bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fecal microbiota transplantation combined with ruxolitinib as a salvage treatment for intestinal steroid-refractory acute GVHD
Authors: Liu Yin , Zhao Ye , Qi Jiaqian , Ma Xiao , Qi Xiaofei , Wu Depei , Xu Yang 
Published: November 2022

AbstractAcute graft-versus-host disease (aGVHD), especially intestinal aGVHD, is one of the most severe complications after allogeneic hematopoietic stem cell transplantation (HSCT). Fecal microbiota transplantation (FMT) has been applied to the treatment of intestinal steroid-refractory aGVHD (SR-aGVHD). Ruxolitinib is the first drug recommended for SR-aGVHD. Here, we reported the outcome data from 21 patients who had received the combined treatment of FMT with ruxolitinib as a salvage treatment in intestinal SR-aGVHD after HSCT. The overall response rate on day 28 was 71.4% (95% CI 50.4–92.5%), including 10 patients with complete responses. The durable overall response at day 56 in responders was 80%. GVHD relapse rate was 33.3% in responders. The levels of inflammatory cytokines as well as T cells and NK cells activation declined. The diversity of the intestinal microbiota was improved in responders. Viral reactivations and severe cytopenia were the major adverse events (61.9% and 81% respectively). The estimated 6-month overall survival was 57.1% (95% CI: 35.9–78.3%), while event-free survival was 52.4% (95% CI: 21.7%-64.1%). Collectively, FMT with ruxolitinib could be an effective treatment for intestinal SR-aGVHD after HSCT.Trial registration: ClinicalTrials.gov identifier: NCT03148743.
Tobacco smoking associates with NF1 mutations exacerbating survival outcomes in gliomas
Authors: Li Xi , Yan Han , Wu Jun , Zhang Longbo 
Published: November 2022

AbstractTobacco smoking is associated with increased risks of nearly 20 types of cancer. Although the association between smoking and gliomas, the most prevalent type of adult brain tumor, is still unconclusive, here, we found that the frequency of NF1 mutations was significantly increased in the glioma patients with smoking history compared to non-smoking patients (24% vs. 10%, P = 0.021). NF1 acts as a tumor suppressor gene is highly mutated in gliomas. The TCGA data analysis indicated that glioma patients carrying NF1 somatic mutations have worse overall survival (median survival time: smoking 19.9 months vs. non-smoking 36.8 month; P = 0.0018). In addition, we revealed that the NF1 and IDH1 mutations were mutually exclusive suggesting NF1 mutation has independent molecular mechanism involved in glioma biology.
Green tea catechin-grafted silk fibroin hydrogels with reactive oxygen species scavenging activity for wound healing applications
Authors: Lee Gyeongwoo , Ko Young-Gwang , Bae Ki Hyun , Kurisawa Motoichi , Kwon Oh Kyoung , Kwon Oh Hyeong 
Published: November 2022

Abstract

Background

Overproduction of reactive oxygen species (ROS) is known to delay wound healing by causing oxidative tissue damage and inflammation. The green tea catechin, (–)-Epigallocatechin-3-
O
-gallate (EGCG), has drawn a great deal of interest due to its strong ROS scavenging and anti-inflammatory activities. In this study, we developed EGCG-grafted silk fibroin hydrogels as a potential wound dressing material.

Methods

The introduction of EGCG to water-soluble silk fibroin (SF-WS) was accomplished by the nucleophilic addition reaction between lysine residues in silk proteins and EGCG quinone at mild basic pH. The resulting SF-EGCG conjugate was co-crosslinked with tyramine-substituted SF (SF-T)
via
horseradish peroxidase (HRP)/H
2
O
2
mediated enzymatic reaction to form SF-T/SF-EGCG hydrogels with series of composition ratios.

Results
Interestingly, SF-T70/SF-EGCG30 hydrogels exhibited rapid in situ gelation (< 30 s), similar storage modulus to human skin (≈ 1000 Pa) and superior wound healing performance over SF-T hydrogels and a commercial DuoDERM® gel dressings in a rat model of full thickness skin defect.

Conclusion
This study will provide useful insights into a rational design of ROS scavenging biomaterials for wound healing applications.
Codesigning simulations and analyzing the process to ascertain principles of authentic and meaningful research engagement in childhood disability research
Authors: Micsinszki Samantha K. , Tanel Nadia L. , Kowal Julia , King Gillian , Menna-Dack Dolly , Chu Angel , Phoenix Michelle 
Published: November 2022

Abstract
Background
Including youth with disabilities and their families as partners in childhood disability research is imperative but can be challenging to do in an authentic and meaningful way. Simulation allows individuals to learn in a controlled environment and provides an opportunity to try new approaches. The objectives of the research study were to (1) codesign a suite of simulations and facilitation resources and understand how stakeholders engaged in the codesign process; and (2) describe the principles of authentic and meaningful research engagement as identified by stakeholders.

Methods
Interdisciplinary stakeholder groups, including youth with disabilities, parents, researchers, and trainees, codesigned simulation training videos by developing shared storylines about challenges with research engagement that were then performed and digitally recorded with standardized patient actors. Two forms of data were collected: (1) observations via field notes and video recordings were analyzed to understand the codesign process; and (2) interviews were analyzed to understand principles of authentic and meaningful engagement.

Results
Four simulation training videos were developed, and topics included: (1) forming a project team; (2) identifying project objectives and priorities; (3) reviewing results; and (4) navigating concerns about knowledge translation. Thirteen participants participated in the simulation codesign; nine of whom consented to be observed in the codesign process and seven who completed follow up interviews. We identified two themes about authentic and meaningful engagement in research: (1) whether the invitation to engage on a project was authentic and meaningful or was extended to ‘tick a box’; and (2) whether there were authentic and meaningful opportunities to contribute (e.g., valued contributions aligned with people’s lived experience, skills, and interests) or if they only served as a ‘rubber stamp’. Communication and expectations tied the ‘tick box’ and ‘rubber stamp’ themes together and underlie whether engagement was authentic and meaningful.

Conclusions
For research engagement to be authentic and meaningful, researchers and families need to set clear expectations, build rapport, have tangible supports, use clear communication, and build time and space to work together. Future work will explore the utility of the simulations and whether they improve knowledge and attitudes about authentic and meaningful engagement in research.
Ultrasound with shear wave elastography in diagnosis and follow-up of common extensor tendinopathy in cases with lateral epicondylitis: a cross-sectional analytic study
Authors: Elsayed Mennatallah , Hafez Moustafa Raafat Mahmoud , Ibrahim Mahmoud Ahmed Hany 
Published: November 2022

Abstract
Background
Lateral epicondylitis (LE) is a common non-traumatic condition. The diagnosis of LE is typically made clinically. Some lateral epicondylitis patients can profit from supplementary imaging for a precise differential diagnosis. Recently, shear wave elastography has been increasingly attracting public attention in evaluation of tendon pathology and tissue elasticity quantitatively. The purpose of our study was to prove that shear wave elastography can be utilized in the diagnosis and follow-up of lateral epicondylitis.

Results
This cross-sectional analytic study involved 42 patients with unilateral lateral epicondylitis (30 males, 12 females with age range: 30–50 years, mean age: 39.9 ± 6 SD). The patients were reviewed by two radiologists with experience of more than 10 years, blinded to each other's results. Lateral epicnodylitis was diagnosed based on clinical criteria. The thickness of common extensor tendon and shear wave speed (SWS) were acquired in elbows bilaterally, along with values of the involved elbows in pre- and post-treatment phases. The comparison between examined groups, inter-rater and intra-rater concordance, and the diagnostic performance have been investigated with paired t-test, an intraclass correlation coefficients (ICCs), and a receiver operator characteristic curve, respectively. The patients with lateral epicondylitis showed a significantly decreased value of shear wave speed on affected side in comparison to the healthy side (P value: 0.000). The shear wave speed of diseased elbows has increased significantly following non-operative management than before therapy. The inter-rater and intra-rater concordance showed both excellent values (ICCs ranged from 0.939 to 1.000) for shear wave speed measurements. Furthermore, a 10.72 m/s cutoff limit of mean SWS (shear wave speed) for differentiating lateral epicondylitis elbows from healthy elbows showed a sensitivity and specificity of 90.5% for both.

Conclusions
Shear wave elastography can be of value as a technique with proper reproducibility and proper diagnostic performance for evaluation and monitoring the therapeutic effect in patients with lateral epicondylitis.
Real-World Evidence of Health Outcomes Related to Lung Stereotactic Body Radiation Therapy in Brazil
Authors: Faroni Lilian , Ferreira Carlos Gil , Moraes Fabio , Baldotto Clarissa , Zukin Mauro , Aran Veronica , Araujo Luiz Henrique 
Published: November 2022

PURPOSE Stereotactic body radiation therapy (SBRT) is an effective option for patients with both early-stage and oligometastatic non–small-cell lung cancer (NSCLC). However, data from Latin America are limited. Therefore, the aim of this study was to investigate the real-world outcomes of applying SBRT for lung lesions in a Brazilian institution. METHODS This study investigated a consecutive cohort of patients treated with SBRT for lung lesions (primary and metastasis). The study primary outcome was local control rates per lesion. Secondary outcomes included progression-free survival (PFS), overall survival (OS), and toxicity. RESULTS Between 2015 and 2019, a total of 216 patients received SBRT and were included in the study. The median follow-up was 24.5 months (5-70), primary NSCLC corresponded to 70% (n = 151) and nonprimary lung lesions to 30% (n = 65), respectively. Stage I NSCLC represented 56% (85 of 151) of the NSCLC cohort. The average number of fractions and total dose prescribed was 5 (3-10)/59 Gy (50-62 Gy). For stage I NSCLC (all lesions treated with a biologically effective dose [10] > 100 Gy), 2-year local control, OS, and PFS were 93.4%, 81.6%, and 80.7%, respectively. For stage IV lesions, if biologically effective dose (10) > 100 Gy or < 100 Gy, 2-year local control was 95.8/86.4% ( P = .03), 2-year-OS was 81.6/60.5% ( P = .006), and 2-year PFS was 38.9/17.9% ( P = .10). Late toxicity was observed in 16.2% (n = 35) of the total cases. CONCLUSION Our results indicate that SBRT is effective (high local control and acceptable toxicity) for treating malignant lung lesions in a real-world scenario in Latin America.
The extracellular matrix controls stem cell specification and crypt morphology in the developing and adult mouse gut
Authors: Ramadan Rana , Wouters Valérie M. , van Neerven Sanne M. , de Groot Nina E. , Garcia Tania Martins , Muncan Vanessa , Franklin Olivia D. , Battle Michelle , Carlson Karen Sue , Leach Joshua , Sansom Owen J. , Boulard Olivier , Chamaillard Mathias , Vermeulen Louis , Medema Jan Paul , Huels David J. 
Published: November 2022

ABSTRACT
The rapid renewal of the epithelial gut lining is fuelled by stem cells that reside at the base of intestinal crypts. The signal transduction pathways and morphogens that regulate intestinal stem cell self-renewal and differentiation have been extensively characterised. In contrast, although extracellular matrix (ECM) components form an integral part of the intestinal stem cell niche, their direct influence on the cellular composition is less well understood. We set out to systematically compare the effect of two ECM classes, the interstitial matrix and the basement membrane, on the intestinal epithelium. We found that both collagen I and laminin-containing cultures allow growth of small intestinal epithelial cells with all cell types present in both cultures, albeit at different ratios. The collagen cultures contained a subset of cells enriched in fetal-like markers. In contrast, laminin increased Lgr5+ stem cells and Paneth cells, and induced crypt-like morphology changes. The transition from a collagen culture to a laminin culture resembled gut development in vivo. The dramatic ECM remodelling was accompanied by a local expression of the laminin receptor ITGA6 in the crypt-forming epithelium. Importantly, deletion of laminin in the adult mouse resulted in a marked reduction of adult intestinal stem cells. Overall, our data support the hypothesis that the formation of intestinal crypts is induced by an increased laminin concentration in the ECM.
Transgenic force sensors and software to measure force transmission across the mammalian nuclear envelope in vivo
Authors: Fenelon Kelli D. , Thomas Evan , Samani Mohammad , Zhu Min , Tao Hirotaka , Sun Yu , McNeill Helen , Hopyan Sevan 
Published: November 2022

ABSTRACT
Nuclear mechanotransduction is a growing field with exciting implications for the regulation of gene expression and cellular function. Mechanical signals may be transduced to the nuclear interior biochemically or physically through connections between the cell surface and chromatin. To define mechanical stresses upon the nucleus in physiological settings, we generated transgenic mouse strains that harbour FRET-based tension sensors or control constructs in the outer and inner aspects of the nuclear envelope. We knocked-in a published esprin-2G sensor to measure tensions across the LINC complex and generated a new sensor that links the inner nuclear membrane to chromatin. To mitigate challenges inherent to fluorescence lifetime analysis in vivo, we developed software (FLIMvivo) that markedly improves the fitting of fluorescence decay curves. In the mouse embryo, the sensors responded to cytoskeletal relaxation and stretch applied by micro-aspiration. They reported organ-specific differences and a spatiotemporal tension gradient along the proximodistal axis of the limb bud, raising the possibility that mechanical mechanisms coregulate pattern formation. These mouse strains and software are potentially valuable tools for testing and refining mechanotransduction hypotheses in vivo.
Erythrocyte sedimentation rate in COVID-19 patients treated at the CLAS health center, Nuevo Lurín, 2021
Authors: Esteves Pairazaman Ambrocio Teodoro , Parren̈o Tipian Juan Manuel , Arbieto Mamani Oscar , Leȯn Mejia Enrique , Esteves Cardenas Veronica Liset , Ñañez del Pino Daniel , Ramos Jaco Antonio Guillermo , Ibarra Fretell Walter Gregorio , CRUZ MAMANI MARIBEL , ZAMBRANA ALFONZO JACQUELINE TERESA 
Published: November 2022

Background: The erythrocyte sedimentation rate (ESR) is used for diagnosis in inflammatory processes, including during the coronavirus disease 2019 (COVID-19) pandemic affecting our population. To determine the values of ESR in the evolution of patients with COVID-19 undergoing treatment at the CLAS Health Center in Nuevo Lurín. Methods: Our research applied a quantitative-observational, descriptive, and longitudinal approach. The sample consisted of 26 male and 19 female patients treated at the study site between February and June 2021, none of whom were in critical condition. To collect all the necessary information, the Capillary or Wintrobe method was used to determine the values of ESR in the evolution of the COVID-19 patients, obtaining different values for the 45 patients seen at the study site. The normal ESR value was 0 - 15mm/h for males and 0 - 20mm/h for females. Results: The initial ESR test in patients positive for COVID-19 showed that only two cases (0.04%) had slightly elevated ESR ranges, while in the rest of the values (95.56%) remained within the normal range. Conclusions: From the results of the capillary velocity of sedimentation method, it can be concluded that patients with COVID-19 in Peru showed no signs of inflammation within their bodies due to the virus.
PD-L1 expression as predictor of immunotherapy eligibility in penile squamous cell carcinoma patients
Authors: Haritsyah Warli Muhammad , Prapiska Fauriski Febrian , Siregar Ginanda Putra , Sihombing Bungaran , Warli Syah Mirsya 
Published: November 2022

Background: Penile cancer is a rare malignancy and potentially lethal disease with an incidence of 0,6-2,1 per 100.000. Squamous cell carcinoma (SqCC) is the most commonly found penile malignancy. PD-L1 is a tumor marker that co-stimulates the receptor PD-1 to suppress T-cell-mediated antitumor immunity. Methods: This study is a retrospective cohort study with a total sampling method. The slides taken from the biopsies of seventy-six male patients from Haji Adam Malik Hospital diagnosed with penile squamous cell carcinoma who have already undergone penile biopsy were re-examined for this study, and PD-L1 levels were measured accordingly. Statistical methods were used to assess the association between PD-L1 levels and with SqCC stage. Results: A total of 76 male patients are the subjects of this study. PD-L1 positivity is identified in 25 patients with +1 intensity in 10 patients (13,2%), +2 in 7 patients (9,2) and +3 intensity in 8 patients (10,5%). There are 36 patients (47,4%) diagnosed with stage T3 SqCC, 35 patients (46,1%) with stage N2 SqCC, and 10 patients (13,2%) with stage M1 SqCC. There is significant correlation between PD-L1 expression and metastasis (p=0,022). However, there is no significant correlation between PD-L1 expression and stage N tumor (p=0,167). Conclusions: PD-L1 highly expressed in advanced stage penile SqCC (32.9%), which is associated with the high-risk clinicopathologic features and poor clinical outcomes. These findings showed a potential usage of immunotherapy in advanced penile SqCC treatment.
Diagnosis of malignant bone tumors in children
Authors: Małek Anna , Wysokińska Olga , Iwaniszyn-Zapołoch Klara , Zadrożna Karolina , Sidorczuk Piotr , Fabiś Mateusz , Wójcik Bartłomiej , Żyga Justyna , Klajda Katarzyna 
Published: November 2022

Bone cancers occur mainly in adolescents and young adults, and benign lesions are more frequent than malignant ones. The most common malignant tumors include osteosarcoma, Ewing's sarcoma and chondrosarcoma. The course of the disease may not be obvious and symptoms may be ambiguous, so it is important to catch them early and take appropriate action. Laboratory tests such as CRP, ESR, LDH, ALP may prove useful in the diagnosis of bone neoplasms in children. All children with suspected malignant bone cancer should undergo an x-ray examination. MRI, CT scans are performed to expand the diagnosis and determine the extent of the disease. The basis of diagnosis is histopathological examination. The result of this study is very important in the selection of therapeutic procedures, it influences decisions regarding the scope and degree of radicality of the operation. It is crucial that the entire diagnostic process is carried out in highly specialized centers that have extensive experience in the diagnosis and treatment of malignant bone tumors in children. It is most beneficial to conduct diagnosis and treatment at the same center, and preferably by the same team of specialists. This team should include doctors from orthopedics, radiology, oncology, radiation therapy and pathomorphology. Conducting diagnosis and treatment in highly specialized centers has a positive effect on the prognosis and quality of life of patients.
The purpose of this article is to highlight the early and uncharacteristic symptoms of bone cancer in children and to present the complexity of the diagnostic process, as well as the importance of carrying it out in highly specialized centers with extensive experience in treating pediatric bone malignancies.
La relation thérapeutique comme chemin de reconnaissance : une lecture éthico-médicale du Parcours de la reconnaissance de Paul Ricoeur
Authors: Tambou Kamgue Ericbert 
Published: November 2022

L’intérêt de Ricoeur pour les questions de bioéthique n’est plus à démontrer. L’objectif de cet article est de procéder, à partir des intuitions ricœuriennes sur la reconnaissance, à une herméneutique de la relation de soin en montrant que celle-ci peut être comprise également comme un parcours de la reconnaissance que l’on se situe du côté soignant ou de celui du patient. La reconnaissance déchaine ce mouvement dans lequel soignant et patient assument le défi du vivre ensemble dans le respect de l’autonomie de l’autre.
Hybrid time–frequency domain dual-probe coherent anti-Stokes Raman scattering for simultaneous temperature and pressure measurements in compressible flows via spectral fitting
Authors: Retter Jonathan E. , Koll Matthew , Dedic Chloe E. , Danehy Paul M. , Richardson Daniel R. , Kearney Sean P. 
Published: November 2022

We demonstrate a hybrid time–frequency spectroscopic method for simultaneous temperature/pressure measurements in nonreacting compressible flows with known gas composition. Hybrid femtosecond–picosecond, pure-rotational coherent anti-Stokes Raman scattering (CARS), with two independent, time-delayed probe pulses, is deployed for single-laser-shot measurements of temperature and pressure profiles along an

∼

5

-mm

line. The theory of dual-probe CARS is presented, along with a discussion of the iterative fitting of experimental spectra. Temperature is obtained from spectra acquired with an early, near-collision-free probe time delay (

τ
1

=

0

p
s

) and pressure from spectra obtained at probe delays of

τ
2

=

150

−

1000

p
s

, where collisions significantly impact the spectral profile. Unique solutions for temperature and pressure are obtained by iteratively fitting the two spectra to account for small collisional effects observed for the near zero probe delay spectrum. A dual-probe pure-rotational CARS system, in a 1D line-imaging configuration, is developed to demonstrate effectively the simultaneous temperature and pressure profiles recorded along the axial centerline of a highly underexpanded jet. The underexpanded air jet permits evaluation of this hybrid time–frequency domain approach for temperature and pressure measurements across a wide range of low-temperature–low-pressure conditions of interest in supersonic ground-test facilities. Single-laser-shot measurement precisions in both quantities and pressure measurement accuracy are systematically evaluated in the quiet zone upstream of the Mach disk. Precise thermometry approaching 1%−2% is observed in regions of high CARS signal-to-noise ratios. Pressure measurements are optimized at probe time delays where the ratio of the late probe delay to the Raman lifetime exceeds four (

τ
2

/

τ
R

>

4

). The impact of low-temperature Raman linewidths on CARS pressure measurements is evaluated, and comparisons of CARS pressures obtained with our recent low-temperature pure-rotational Raman linewidth data and extrapolated high-temperature

Q

-branch linewidths are presented. Considering all measurements with

τ
2

/

τ
R

≥

4.0

, measured pressures were on average 7.9% of the computed isentropic values with average shot-to-shot deviations representing a combination of instrument noise and fluid fluctuations of 5.0%.