bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation of rare Genera of actinomycetes — antibiotic producers from soils using Aloe Arborescens juice
Authors: Sineva O. N. 
Published: January 2022

The search for new antibiotics is an urgent problem due to the spread of resistance to existing antibacterial drugs in pathogenic microorganisms. Actinomycetes are producers of a large number of antibiotics used in medicine. Most antibiotics are isolated from actinomycetes of the Streptomyces genus, while rare genera of actinomycetes can be the producers of new antibiotics.The aim of the study is to investigate the effect of the biological substances complex present in aloe juice on the growth stimulation of rare genera of actinomycetes.Material and methods. Objects: samples of sod-podzolic soil and chernozem. The standard method of sowing soil suspensions on oat agar and Gause medium No. 2 was used to isolate actinomycetes. Chemotaxonomic properties were determined using the methods of ascending thin-layer chromatography on a cellulose layer. The generic identity of cultures was determined using Bergey’s manual and materials comparing the composition of cell walls of actinobacteria. DNA PCR with standard 27f and 1492r primers, as well as Sanger sequencing, were performed to study genosystematic features. Antibiotic activity was determined against the test microorganisms: Staphylococcus aureus ИНА 00985 (FDA 209P), Staphylococcus aureus ИНА 00761 (MRSA), Staphylococcus aureus ИНА 00762 (УФ- 2), Micrococcus luteus ATCC 9341, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Saccharomyces cerevisiae ИНА 01042.Results. A total of 527 actinomycete cultures were isolated from samples of sod-podzolic soil and chernozem with the addition of aloe juice; their phylogenetic position was determined. The dominant actinomycetes in the studied soil samples are the representatives of the genus Streptomyces. Bacteria of the genus Micromonospora take the second place by the number of isolated cultures. Rare genera of actinomycetes have also been identified: Nonomuraea, Streptosporangium, Nocardia, Actinomadura, Actinocorallia, Pseudonocardia, Amycolatopsis, Saccharomonospora, Saccharopolyspora, Promicromonospora, Kribbella. It was determined that the isolated cultures possess antibiotic activity against test microorganisms.Conclusion. It is advisable to use aloe juice after subjecting the leaves to biostimulation to isolate actinomycetes from the soil and identify their biodiversity.
PERAMALAN KUNJUNGAN WISATAWAN MANCANEGARA DI SUMATERA BARAT PADA SAAT PANDEMI COVID 19
Authors: Yulius Henny 
Published: January 2022

Pada sektor pariwisata jumlah kunjungan wisatawan mancanegara memegang peranan penting terhadap pertumbuhan ekonomi. Untuk meningkatkan kunjungan wisatawan mancanegara perlu dilakukan pembangunan yang berkelanjutan yang sejalan dengan tren pertumbuhan kunjungan wisatawan mancanegara agar pembangunan tepat sasaran, efektif dan efisien. Penelitian ini bertujuan untuk meramalkan kunjungan wisatawan mancanegara ke Sumatera Barat menggunakan metode peramalan regresi linear. Data historis yang digunakan adalah data kunjungan wisatawan mancanegara ke Sumatera Barat pada Tahun 2018 sampai tahun 2020 dari Badan Pusat Statistik (BPS). Hasil dari penelitian adalah peramalan jumlah kunjungan wisatawan asing di Sumatera Barat berdasarkan data historis 6 tahun terakhir menunjukkan bahwa wisatawan asing yang akan datang berkunjung pada tahun 2021 di Sumatera Barat adalah 37.858,47 jiwa.
PERBANDINGAN PEMAKAIAN ROCKER ARM KONVENSIONAL DENGAN ROCKER ARM ROLLER BEARING PADA SEPEDA MOTOR
Authors: Jusnita  
Published: January 2022

Roller Rocker Arm is important for transmitting signals and determining work efficiency which is the result of technological developments from ordinary rocker arms. The purpose of this study was to compare the use of conventional rocker arm with rocker arm roller on power, torque, exhaust emissions, compression on a motorcycle engine. The research method used is changing the fuel system, ignition system, changing the valve mechanism. The maximum torque on the conventional rocker arm occurs at 2995 rpm with a value of 10.92 Nm. Maximum torque decreases with increasing engine speed. The decrease in power at high speed occurs due to the influence of the volume of the fuel and air mixture which tends to decrease. The highest fuel consumption occurs at 7000 rpm engine speed in a conventional rocker arm of 0.124 kg/kWh, so the rocker arm roller is more efficient than the conventional rocker arm. Fuel consumption rocker arm roller rotation 7000 rpm 0.028 kg/kWh. While the conventional rocker arm fuel consumption at 7000 rpm 0.124 kg/kWh. Exhaust emissions, the rocker arm roller is environmentally friendly compared to the conventional rocker arm CO2 rocker arm roller only produces 5.2%, while the conventional rocker arm test results after the average CO2 value reaches 5.3%. The results of testing the two rocker arms on compression are the same at 90 Psi and 6.2kg/ from the standard size of 10-11kg/
Pengukuran Beban Kerja Mental Job Driver Dan Swamper Team Fuel Menggunakan NASA-TLX
Authors: Qurthuby Muhammad 
Published: January 2022

Giving excessive workloads causes work stress both physically and psychologically and emotional reactions. The excess workload experienced by drivers and swampers at PT XYZ results in reduced attention at work, decreased work motivation, and decreased skill levels, thus affecting drivers and swampers productivity and the chance of a work accident is very high. This study aims to measure the Mental Workload of Job Driver and Swampers Fuel Tank Using the NASA-TLX Method. The NASA-TLX score obtained 93.8, driver 2 get 83.7, driver 3 91.3, swamper 1 91, swamper 2 89.5, and swamper 3 94.7. Elements of mental workload that are very influential are Mental Demand with a percentage of 22%, Effort 20%, Physical Demand 18%, Own Performance 15%, Frustation Level 15% and Temporal Demand 12%.
Karakteristik Semen Segar dan Motilitas Spermatozoa Ayam Hutan Merah (Gallus Gallus) dalam Pengencer yang Mengandung Air Tebu
Authors: Arsyad  , Muhammad Erik Kurniawan  , Khaeruddin  , Bahri Syamsuryadi  
Published: January 2022

Penelitian ini bertujuan untuk mengetahui level air tebu terbaik untuk ditambahkan dalam pengencer semen ayam hutan merah berbasis ringer laktat kuning telur. Semen dikoleksi menggunakan metode pemijatan dan semen segar dievaluasi secara makroskopis dan mikroskopis. Semen dibagi ke dalam lima tabung yang masing-masing berisi pengencer ringer laktat kuning telur , ringer laktat kuning telur air tebu 10% , ringer laktat kuning telur air tebu 20% , ringer laktat kuning telur air tebu 30% , ringer laktat kuning telur air tebu 40% . Semen disimpan dalam refrigerator selama 24 jam kemudian dilakukan evaluasi motilitas spermatozoa (progresif, reverse, vibrator, total). Hasil penelitian menunjukkan bahwa perlakuan level air tebu dalam pengencer berpengaruh sangat nyata (P<0.01) terhadap motilitas progresif, motilitas reverse dan motilitas total. Penambahan level air tebu 10% dan 20% menghasilkan motilitas total paling tinggi yaitu 86.24-88.79%. Namun, motilitas progresif tertinggi didapatkan pada penggunaan level air tebu 20% yaitu 77.67%. Kesimpulan penelitian ini adalah air tebu 20% dapat ditambahkan dalam pengencer ringer laktat kuning telur untuk mempertahankan motilitas total dan motilitas progresif spermatozoa ayam hutan merah selama penyimpanan 24 jam.
LA INTEGRACIÓN DE DATAMART CON DATAWAREHOUSE
Authors: Avila Cruz Carlos Ariel , Chiquito Muñiz Jair Josue 
Published: January 2022

Este trabajo de investigación se basó sobre la integración de datamart con datawarehouse como su posible implementación ya que en nuestra documentación se encontró evidencia que la integración del base de datos en este caso las datamart deben ser realizadas de una forma correcta y aquí así se tiene una mayor integración de datos para los usuarios que la utilice y se ha difícil la pérdida información ya que un datawarehouse es un repositorios de información, el actualidad existen muchos programas que nos permite realizar la sincronización información de un datamart con el datawarehouse de una formas rápida y sencilla. Aunque no todas la base datos nos brindar seguridad al momento de realizar la integración de datos por este motivo hay que poner en cuenta varios factores Para realizar este artículo científico se realizó algunos métodos metodológicos como son los siguientes: Histórico-lógico, Análisis-Síntesis entre otros ya ayudaron mucho en la búsqueda de información y la vez tener un mejor conocimiento del tema cómo es la integración de datamart con datawarehouse
LAS REDES WAN Y SU IMPORTANCIA PARA LOS ORDENADORES
Authors: Rodriguez Toala Bryan Antonio , Pincay Segovia Eddy José , Maldonado Zúñiga Kirenia 
Published: January 2022

El presente trabajo de investigación dedicado a la Las redes WAN y su importancia para los ORDENADORES debería ser hoy en día una inquietud importante para cualquier empresa organización donde se beneficia en el ámbito tecnológico y laboral Las redes WAN se utilizan principalmente para enviar información a grandes distancias y mantener interconectadas ciudades, países y continentes, permitiendo un flujo de información de alta velocidad.La generalidad de la computadora personal y de la red hace ha dado lugar a posibilidades para poder acceder a la información de actual, podemos cargar aplicaciones desde cualquier punto, compartir y recibir mensajes de cualquier parte del mundo, todo tan solo desde un equipo y en segundos. Los equipos modernos en la actualidad gracias a su complejidad nos permiten las redes y es tan eficaz debido a la diversidad de sus componentes. A nivel mundial se ha implantado una red de computadoras que se considera como uno de los más grandes avances de la tecnología en las últimas décadas. Su importancia más relevante, es que mantienen la información en constante movimiento y al alcance de todos, ya que sin estas redes, la información estaría ubicada solamente en las redes locales y no pudieran ser accedidas por usuarios que se encuentren a kilómetros de distancia. Sin las redes WAN, no existiría Internet ni acceso público a la información, es por esta razón, que es muy importante la presencia de redes WAN en el mundo. Para cuando se allá identificado el tipo de conexión que tendrá
Preparation and Thickness Optimization of Graphenic-Based Carbon Material as a Microwave Absorber
Authors: Kurniawan Affandi Faisal , Anwar Mohammad Syaiful , Nadiyyah Khoirotun , Taryana Yana , Ramli Muhammad Mahyiddin , Mashuri Mashuri , Triwikantoro Triwikantoro , Darminto Darminto 
Published: January 2022

The purpose of this study is to optimize the thickness of a layered graphenic-based carbon compound, which is a non-magnetic material derived from biomass (old coconut shell). After the sample was exfoliated using HCl solution, the morphological structure showed that the material used in this study is a reduced graphene oxide (rGO), similar to carbon but with a thickness of less than 10 nm and lateral size in submicron (100 nm). The sample with a 2 mm thickness was then characterized using a vector network analyzer (VNA) to measure its reflection loss (RL). The measurement result is evaluated by converting the S-parameter values (S11 and S21) from the VNA using the Nicolsson Ross Weir (NRW) method to obtain input variables such as relative complex permeability and relative complex permittivity. Following this, the single-layer thickness of the sample was optimized using a genetic algorithm (GA), which can predict the appropriate thickness so that the optimum RL can be obtained. The optimum thickness of the sample was found to be 3.48 mm, which resulted in a much higher RL. The RL was re-measured for verification using a sample with the corresponding optimized thickness, revealing that this optimization is feasibly operational for a radar absorbing material (RAM) design.
HIGHLIGHTS

Carbon compounds containing graphenic phase derived from coconut shell are functional materials having various unique properties such as superior electrical conductivity, large surface area, and excellent structural flexibility, and microwave absorbtion
The single-layer microwave absorber employing carbon compounds has been prepared
The layer thickness optimized using a genetic algorithm (GA) can estimate the appropriate design with the maximum reflection loss (RL)
The Role of Ferroptosis-Related Gene in the Immune Activity and Prognosis of Sepsis
Authors: Dai Wei , Tian Yu , Luo Deqiang , Xie Qian , Liu Fen , Shao Qiang , Zhao Nin , Zheng Ping , Qian Kejian 
Published: January 2022

IntroductionSepsis is a leading cause of mortality in intensive care units worldwide. Ferroptosis, a form of regulated cell-death–related iron, has been proven to be altered during sepsis, including increased iron transport and uptake into cells and decreased iron export. A better understanding of the role of ferroptosis in sepsis should expedite the identification of biomarkers for prognostic evaluation and therapeutic interventions.Material and methodsWe used the mRNA expression profiles of sepsis patients from Gene Expression Omnibus (GEO) to analyze the expression level of ferroptosis-related genes and construct molecular subtypes.ResultsTwo distinct ferroptosis patterns were determined, and the overall survival of the two clusters was highly significantly different. Gene comparison analysis was performed on these two groups, and there were a total of 106 differentially expressed genes(DEGs). Pathway enrichment analysis of these genes showed that ferroptosis and immune-related pathways were enriched, suggesting that immune pathways might be critically involved in sepsis. To systematically predict the prognosis of sepsis, we constructed a nomogram model, the calibration plot nomogram showed excellent concordance for the 7-, 14-, and 28-days predicted and actual overall survival probabilities. Finally, the results of bioinformatics analysis were validated in animal and cell modelsConclusionsIn this study, we construct a ferroptosis-related nomogram that can be used for prognostic prediction in sepsis. In addition, we revealed the ferroptosis played a non-negligible role in immune cell infiltration and guiding more effective immunotherapy strategies.
Masquelet technique in management of infective non-union of long bones- A prospective study
Authors: Loganathan Senthil , Murali Sradha , Thiyagarajan U. , Gokulraj D. 
Published: January 2022

Introduction and Aim: Masquelet’s technique is a 2-staged procedure, for treatment of infected segmental bone defect. 1st stage involves radical debridement with antibiotic-induced cement spacer. During second stage, the spacer is removed and the autologous bone graft is applied into the biomembrane formed. In this study, we evaluate the Masquelet’s technique for the management of infective non-union of long bones.

Materials and Methods: 15 patients with infective non-union of long bones- tibia, femur and a case of congenital pseudoarthrosis of tibia, were treated with Masquelet’s technique. They underwent 2 stages of procedures 6-8 weeks apart and was followed up for about 9 months and radiological and clinical outcomes were assessed.

Results: Out of 15 patients with infective non-union, 8 patients attained union. Out of the 7 patients with failure of the technique, higher failure rates were attributed to Pseudomonas infection.

Conclusion: Masquelet’s technique is a cost-effective method for treating infective segmental non-unions, not requiring special training or sophisticated instruments. This method shows good results with Gram positive infections. Although, the outcome with Pseudomonas aeruginosa infection, have not shown satisfactory results.
Alterations of liver function tests in diabetic obese and nonobese individuals
Authors: S. Avinash , Silambanan Santhi 
Published: January 2022

Introduction and Aim: India has emerged as the diabetes capital in Southeast Asia having around 74 million with diabetes, with a prevalence of 9.8% in 18–99 years of age. In type 2 diabetes mellitus, triglycerides get deposited in liver thus altering its structure and function, which is the feature of nonalcoholic fatty liver disease. This study was undertaken to study the alterations in liver function tests in obese and nonobese diabetic individuals.

Materials and Methods: The Department of Biochemistry at Sri Ramachandra Institute of Higher Education and Research was chosen to conduct the retrospective study on 200 diabetic individuals from September 2019 to February 2020. The data obtained were serum liver function tests, HbA1c, plasma glucose and lipid profile. Before the study ethics approval was obtained from the Institutional Ethics Committee for studies involving human participants. The obtained data were subjected to statistical analysis using SPSS version 16 and a P value less than 0.05 was considered statistically significant.

Results: Transaminases and ALP were significantly altered in obese diabetics; were positively correlated with bilirubin. TGL was negatively correlated with AST/ALT ratio.

Conclusion: Liver enzymes and bilirubin were altered in obese diabetics. Measurement of liver function biomarkers are cost effective diagnostic markers of non-alcoholic fatty liver disease.
Thyroid autoimmunity among type 2 diabetes mellitus: assessing anti-thyroid peroxidase (anti-TPO) antibodies
Authors: Sah Narendra Kumar , Deo Saurabh Kumar , Walia Harpreet Kaur , Singh Alka , Prasad Suvarna , Kaur Kiranjeet 
Published: January 2022

Introduction and Aim: Thyroid disorder, the most abundant endocrine disorder in the globe ranks second after diabetes mellitus. Because of immune resistance to thyroid gland, it leads to the development of hyperthyroidism and hypothyroidism. Autoantibodies against thyroid peroxidase (TPO) - a critical enzyme in the thyroid hormone biogenesis is responsible for thyroid autoimmunity and is increasingly found among type 2 diabetes mellitus (T2DM). So, the present study was designed to assess the serum anti-TPO antibodies among type 2 diabetes mellitus in hospital setting.

Materials and Methods: 100 clinically diagnosed type 2 diabetes mellitus was included with 50 age and sex matched apparently healthy controls in the age range 35-70 years. Mean age (in Years) of male and female T2DM cases had 57.40±5.93 and 55.96±7.39 respectively. Also, the male and female controls had mean age as 56.24±8.74 and 54.20±8.90 respectively.

Results: The mean glycated hemoglobin (HbA1c) value among T2DM male and female cases was found to be 8.53±2.02% and 8.66±1.90% respectively. The mean value of serum TSH was found to be highly significant statistically among T2DM as compared to healthy controls, whereas mean value of serum anti-TPO antibodies was significant statistically among T2DM as compared to healthy controls. The occurrence of anti-TPO antibodies among T2DM was found to be 13 out of 100 (13%) in the present study.

Conclusion: The association between serum TSH and anti-TPO antibodies among T2DM patients was found to be highly significant statistically. Thus, the existence of higher anti-TPO antibodies among T2DM points out the future development of functional thyroid problem in such patients.
Cytokine profile as biomarker of COVID-19 and its correlation with liver function enzymes and other markers of inflammation
Authors: Nepal Gita , D. S. Maitreyee , Aroor Akshatha Rao , A. R. Shivashankara 
Published: January 2022

Introduction and Aim: Various hematological and biochemical parameters are assessed as biomarkers of COVID-19, caused by the SARS-CoV-2. In this study, efforts were made to identify the correlation of cytokines (IL-6, TNF-?, IL-1?, and IL-1?) with the liver function enzyme markers, LDH, CRP, Ferritin, and D-dimer. We also assessed the correlation of cytokines with severity of COVID-19.

Materials and Methods: We analyzed 53 serum samples of RT-PCR-positive patients admitted to the hospital. Cytokines (IL-6, TNF-?, IL-1?, and IL-1?) were analyzed with sandwich ELISA. The levels of cytokines were compared between mild, moderate, and severe cases of COVID-19, and the correlation among cytokines, liver function marker enzymes, LDH, CRP, D-dimer, and ferritin were analyzed.

Results: Along with CRP, LDH and ferritin, IL-6 showed significant difference between mild, moderate, and severe COVID groups. significant correlation between IL-6 & LDH (p -.002), IL-6 & D-Dimer (p- .010), IL-6 & IL-1? (p- .027), IL-1? & D-Dimer (p- .010), IL-1? & LDH (p-.027), and IL-1? & TNF-? & (p-.000).

Conclusion: Cytokines especially IL-6 correlated with disease severity. Assaying the profile of cytokines could be of immense value in diagnosis, prognosis, and management of COVID-19.
HIV Myelopathy- A cross-sectional study of constellation of bone marrow findings in HIV/AIDS
Authors: Kuppan Hemalatha , Ashok Vanishri , Hanaganahalli Basavaiah Sridevi , Niranjan Khadilkar Urmila , Murali Nirupama 
Published: January 2022

Introduction and Aim: Haematological manifestations in HIV disease is common and can happen at any phase during the disease course. Anemia and thrombocytopenia are the most frequent hematologic abnormalities and are associated with high morbidity and mortality. The objective of current study was to observe and analyse various spectrum of bone marrow changes and haematological abnormalities in HIV/AIDS and to correlate findings with CD4 count.

Material and Methods: A total of 44 patients over a period of 5 years were included. Clinical findings, hematological profile, bone-marrow aspirate, biopsy findings and CD4 count of these patients were documented. The association between absolute lymphocyte count (ALC) and CD4 count were further established.

Results: The most common clinical indication for bone-marrow aspiration and trephine biopsy was pancytopenia (47.3%), pyrexia of unknown origin (15.1%), and unresolving hepatosplenomegaly (13.6%). Anemia (72.7%) was commonest haematological abnormality. Bone marrow aspirate was normocellular in majority of patients. Marrow findings were correlated with CD4 count and were found to be statistically significant. Tri-lineage dysplasia was observed in 9.1% of patients, and megakaryocytic dysplasia being the commonest(61.4%). Histiocytic aggregates (27.3%) were noted among which 6.8% showed acid fast bacilli in Ziehl-Neelsen stain. Fungal stains revealed histoplasmosis in 4.5% patients.

Conclusion: There was a strong negative association between presence of anemia and dysplasia and CD4 count. When CD4 was <200/µL and ALC<1000/mm3, presence of anemia and dysplasia affecting various cell lines were commonly observed; therefore, can be used as indicators to assess the severity of the disease.