bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design and development of an affordable and washable multilayered mask with a recycled hydrophobic layer for preventive health care
Authors: Sekhar Sugali Chandra , Pabba Manideep , Tallam Aarti , Fatima Saeed , Butti Sai Kishore , Vani Bukke , Sahu Nivedita , Sundergopal Sridhar 
Published: November 2022

Abstract
The sudden onset of Covid-19 pandemic has led to the implementation of various health-care measures, among which the facemask has become the primary line of defense. Several masks available in the market are single-use that would choke the environment through plastic pollution, or too expensive for the commoner to afford. As the pandemic affected population of all races, religions and communities across the globe, there was a need to design a low-cost mask that could be washed and reused multiple times without loss in barrier properties. The present work relates to the design of a facemask that incorporates a waste derived warp knit polyethylene terephthalate (PET) permeate spacer extricated from used reverse osmosis (RO) and other membrane modules. A non-woven polypropylene (PP) layer was incorporated immediately after the PET layer to provide high hydrophobicity and increase the threshold pressure for the respiratory droplets to penetrate. These two active layers were sandwiched between two tightly woven cotton layers as the first and fourth layers. The cotton fabric of the developed mask provides comfort, good appearance, biodegradable nature and tightly woven that reject larger particulate matter including dust. The unique PET layer has high mechanical strength and 3D shape holding capability that extended from the nose bridge to the chin, leaving an air gap between the mouth and the inner layer of the facemask, enabling smooth facial movement, hindrance-free speaking and prevents spectacle fogging. The PP layer is also known to exhibit electrostatic repulsion towards pathogens present in the air and prevents the chances of infections. Compared to commercial N95 mask, the developed mask exhibits high breathability, reusable up to 30 washes and is produced at an affordable cost of Rs.17 (0.22 USD) including labor charges and sold at 100% profit margin @ Rs.35 (0.45 USD) per unit. The developed mask has high bacterial filtration efficiency and prevents respiratory droplets containing infection causing organisms, dust allergies, and those arising from environmental pollution. Moreover, the mask was certified by neutral testing agencies and sold to a population of more than 6 lakhs thus playing a major role in mitigation of Covid-19.
The green synthesis of environmentally friendly magnetic silver complex stabilized on MnFe2O4@sodium alginate nanoparticles (MCF@S-ALG/Ag) and evaluation of their antibacterial activity
Authors: Mohammadizadeh Zahra Najahi , Ahadifar Mitra , Mobinikhaledi Mahta , Ahadi Najmieh 
Published: November 2022

Abstract
Magnetic nanoparticles with green cover sodium alginate and Ag, MnCoFe2O4@Sodium alginate/Ag (MCF@S-ALG/Ag) MNPs, were prepared by a simple and clean method from Sargassum Vulgare brown algae. The structure of these nanoparticles was characterized by the Fourier transform infrared (FT-IR), X-ray powder diffraction (XRD), field emission-scanning electron microscope (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Furthermore, the antibacterial activity of MCF@S-ALG/Ag MNPs was tested for two bacterial of gram-negative (Escherichia coli (E. coli)) bacteria and gram-positive (Staphylococcus aureus (S. aureus)) bacteria. The MCF@S-ALG/Ag MNPs showed the inhibition zone 16.32 mm for S. aureus and 12.84 mm for E. coli bacteria. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of MCF@S-ALG/Ag MNPs for S. aureus bacteria were found 20 µg/mL and 40µg/mL, respectively.
Can outward foreign direct investment improve China's green economic efficiency?
Authors: Wang Chong , Wang Lei 
Published: November 2022

Abstract
Will OFDI help promote the green transition of China's economy? Our study adopts the inter-provincial panel data of China from 2011 to 2019 to explore the influence of OFDI on green economic efficiency (GEE). We find that OFDI significantly improve China's GEE, robustness tests still support this conclusion. We also find that the reverse technology spillover obtained from developed countries through OFDI promotes the growth of GEE. Heterogeneity analysis shows that, OFDI significantly promotes GEE in eastern China, however, this promotion effect is not significant in midwestern China. Besides, the promoting effect of OFDI on GEE has been further improved after 2016. This paper further finds that there exists a threshold effect of OFDI on GEE, when the financial development and human capital exceeds 2.0954 and 0.0290, respectively, the promoting effects of OFDI on GEE are greatly enhanced.
Two gates mediate NMDA receptor activity and are under subunit-specific regulation
Authors: Wollmuth Lonnie P , Amin Johansen , He Miaomiao , Prasad Ramesh , Leng Xialing , Zhou Huan-Xiang 
Published: November 2022

Abstract
The opening and closing kinetics of NMDA receptors (NMDARs) contribute to their unique role in synaptic computation. NMDARs are heterotetramers composed of the obligate GluN1 and typically some combination of GluN2 subunits. The initial step in ion channel opening is agonist binding, which generates free energy that propagates to the ion channel to open a canonical gate at the M3 helix bundle crossing. A conserved glycine in the outer most transmembrane helices, the M4 helix, regulates NMDAR function. Here we find that the GluN1 glycine mainly regulates single channel events within a cluster or bursts of activity, whereas the GluN2A glycine mainly regulates entry and exit from clusters. Molecular dynamic simulations suggest that, whereas the GluN2 M4 (along with GluN2 pre-M1) regulates the gate at the M3 helix bundle crossing, the GluN1 glycine regulates a ‘gate’ at the M2 loop. Subsequent functional experiments support this interpretation. Thus, the distinct kinetics of NMDARs are mediated by two gates that are under subunit-specific regulation.
Novel surface nanoengineering technology for the removal of sulfur compounds associated with negative attributes in wines
Authors: Mierczynska-Vasilev Agnieszka , Kulcsar Allie , Dabare Panthihage , Vasilev Krasimir , Bekker Marlize 
Published: November 2022

Abstract
Volatile sulfur compounds (VSCs), such as hydrogen sulfide, methanethiol, and ethanethiol, are associated with ‘reductive’ aromas in wine and contribute to approximately 30% of all wine faults. These compounds can have a significant impact on wine aroma and perceived quality, and subsequently, consumer preference. In this communication, we report a novel method for the generation of smart surfaces that incorporate immobilized gold nanoparticles to remove VSCs compounds.
Novel insights into the diagnostic and prognostic function of copeptin in cardio-metabolic disorders
Authors: Wu Penglong , Wang Lilan , Cheng Ye , Wang Bin , Su Xin 
Published: November 2022

Abstract
As is shown in previous reports, arginine vasopressin, as one of the most important hormones within circulation in human beings, is of great clinically significance given that it could maintain the body fluid balance and vascular tone. However, the laboratory measurements arginine of vasopressin in daily clinical practice are shown to be difficult and with low accuracy. Concerning on this notion, it is unpractical to use the serum levels of arginine vasopressin in diagnosing multiple diseases. On the other hand, another key serum biomarker, copeptin, is confirmed as the C-terminal of the arginine vasopressin precursor which could be released in equal amounts with arginine vasopressin, resultantly making it as a sensitive marker of arginine vasopressin release. Notably, emerging recent evidence has demonstrated the critical function of copeptin as a clinical indicator, especially in the diagnosis and prognosis of several diseases in diverse organs, such as cardiovascular disease, kidney disease, and pulmonary disease. In addition, copeptin was recently verified to play an important role in diagnosing multiple acute diseases when combined it with other gold standard serum biomarkers, indicating that copeptin could be recognized as a vital disease marker. Herein, in the current review, the functions of copeptin as a new predictive diagnostic and prognostic biomarker of various diseases, according to the most recent studies, are well summarized. Furthermore, the importance of using copeptin as a serum bio-marker in diverse medical departments and the impact of this on improving healthcare service is also summarized in the current review.
Limiting factors for charge generation in low-offset fullerene-based organic solar cells
Authors: Jungbluth Anna , Cho Eunkyung , Privitera Alberto , Kaienburg Pascal , Lauritzen Andreas , Derrien Thomas , Kesava Sameer , Habib Irfan , Coropceanu Veaceslav , Bredas Jean-Luc , Riede Moritz 
Published: November 2022

Abstract

Free charge generation in organic solar cells generally proceeds via (1) the formation of charge transfer (CT) excitons after photoexcitation of donor or acceptor molecules, and (2) CT dissociation into the charge separated (CS) state. While the efficiency of CT formation depends on the energetic difference between local excitation (LE) singlet and CT states, the CT dissociation efficiency is determined by the energetic barrier between the CT and CS states. For a long time, research either studied the combined effect of CT formation and dissociation on photocurrents without distinguishing the individual processes, or primarily focused on understanding and improving CT formation to increase photocurrents, neglecting the efficiency of CT dissociation all-together. In this work, we provide evidence that CT dissociation rather than CT formation presents a major bottleneck for free charge generation in fullerene-based blends with low energetic offsets between LE and CT states. We fabricate devices based on dilute donor content blends of ZnPc or its fluorinated derivatives and C60. Fluorination of ZnPc simultaneously shifts the molecular orbitals away from the vacuum level, increasing the CT state energy at the donor-acceptor interface with C60. Through experimental device characterization, density functional theory (DFT) calculations, and time-resolved electron paramagnetic resonance (trEPR) measurements, we draw a comprehensive picture of how LE, CT, and CS state energies and the transitions among states change upon fluorination of ZnPc. We find that, upon fluorination of ZnPc, primarily CT dissociation decreases, resulting in a significant decrease in the photocurrents of the blends.
Ovine PAPPA2 gene coding variants are linked to decreased fecal egg shedding in native Turkish sheep naturally infected with gastrointestinal nematodes.
Authors: YAMAN Yalçın , Bay Veysel , SEVİM Semih , AYMAZ Ramazan , KELEŞ Murat , Önaldı Abdullah Taner , ÖZÜİÇLİ Mehmet , ŞENLİK Bayram , KONCAGÜL Seyrani , YILMAZ Onur , ÜN Cemal 
Published: November 2022

Abstract
In this study, the association between PAPPA2 coding variants and gastrointestinal (GI) nematode fecal egg count (FEC) score in adult Turkish sheep was investigated. For this purpose, the FEC score was determined in adult sheep from six breeds. Sheep were classified as shedders or non-shedders within breeds and flocks. The first group was the fecal egg shedders (> 50 per gram of feces), and the second group was the no fecal egg shedders (≤ 50 per gram of feces). The exon 1, exon 2, exon 5, exon 7, and a part of 5’UTR of the ovine PAPPA2 gene were genotyped by Sanger sequencing of these two groups). Fourteen synonymous and three non-synonymous single nucleotide polymorphisms (SNPs) were found. The non-synonymous SNPs; D109N, D391H, and L409R variants, are reported for the first time. Two haplotype blocks were constructed on exon 2 and exon 7. The specific haplotype, C391G424G449T473C515A542 on the exon 2 that carries the 391H variant, was tested against four other common haplotypes. Our results indicate that C391G424G449T473C515A542 haplotype was significantly associated with fecal egg shedding status in adult Turkish sheep (p-value, 0.044).
Resolving Atomic-Scale Structure and Chemical Coordination in High Entropy Alloy Electrocatalysts for Structure-Function Relationship Elucidation
Authors: Subhash Bijil , Unocic Raymond , Gallington Leighanne , Wright Joshua , Cheong Soshan , Tilley Richard , Bedford Nicholas 
Published: November 2022

Abstract
The wide range of potential surface configurations in high entropy alloy nanocatalysts (HEAs) offer substantial degrees of freedom to tune their catalytic properties. This complexity in possible atomic arrangements, however, presents a significant challenge in resolving active structural motifs, preventing the establishment of structure-function relationships for rational catalyst optimization. Herein, we present a methodology for creating sub 5 nm HEAs using an aqueous-based peptide-directed route. Using a combination of synchrotron scattering and spectroscopy techniques, HEA structure models are constructed from stochastic modelling of experimental datasets and showcase a clear peptide-induced influence on atomic-structure and chemical miscibility. Analysis of our models allow for the construction of structure-function correlations applied to electrochemical methanol oxidation reactions, revealing the complex interactions between multiple metals that lead to improved catalytic properties. Our results showcase a viable strategy for elucidating structure-function relationship in HEAs, prospectively providing a pathway for future materials design.
TIMELESS promotes the proliferation and migration of lung adenocarcinoma cells by activating EGFR through AMPK and SPHK1 regulation
Authors: Yin Houqing , Wang Zequn , Wang Dan , Nuer Muhadaisi , Han Mengyuan , Ren Peng , Ma Shanwu , Lin Chutong , Chen Jingjing , Xian Haocheng , Ai Dongmei , Li Xuejun , Ma Shaohua , Lin Zhiqiang , Pan Yan 
Published: November 2022

Abstract
Background: Lung adenocarcinoma (LUAD) has high morbidity and is prone to recurrence. TIMELESS (TIM), which regulates circadian rhythms in Drosophila, is highly expressed in various
tumors.
Methods： We used tumor samples from patients with lung carcinoma and LUAD patient data from public databases to confirm the relationship of TIM expression with lung cancer. We used NSCLC cell lines and siRNA to knock down TIM expression, and further analyzed cell proliferation, migration and colony formation. By using western blot and qPCR, we detected the influence of TIM on EGFR, Sphk1 and AMPK. With proteomics analysis, we comprehensively inspected the different changed proteins influenced by TIM and did global bioinformatic analysis.
Results: In this study, we found that TIM expression was elevated in LUAD and that this high expression was positively correlated with more advanced tumor pathological stages and shorter overall and disease-free survival. Moreover, gefitinib efficacy in patients with LUAD could be influenced by TIM expression, and the antitumor effect of gefitinib was significantly improved with TIM knockdown. TIM knockdown inhibited epidermal growth factor receptor (EGFR) activation and phosphorylation of its downstream AKT/mTOR and ERK1/2 pathways. We also clarified that TIM regulated the activation of sphingosine kinase 1 (SPHK1) in LUAD cells, while SPHK1 knockdown inhibited EGFR activation. Quantitative proteomics techniques combined with bioinformatics analysis were adopted to clarify the global molecular mechanisms regulated by TIM in LUAD. The results of proteomics suggested that mitochondrial translation elongation and termination were altered, which were closely related to the process of mitochondrial oxidative phosphorylation. Knockdown of TIM reduced the ATP content and promoted AMP-activated protein kinase (AMPK) activation.
Conclusions: Our study revealed that TIM could regulate EGFR activation through AMPK and SPHK1, as well as influence mitochondrial function and alter the ATP level; thus, TIM is a key factor in LUAD.
Identification of founder and novel mutations that cause congenital insensitivity to pain (CIP) in Palestinian patients
Authors: Khaled Boushra , Alzahayqa Mahmoud , Jaffal Ahmad , Sallam Husam , Thawabta Rua’a , Mansour Mamoun , Salah Zaidoun 
Published: November 2022

Abstract
Congenital insensitivity to pain (CIP) is a rare autosomal recessive disorder, which is characterized primarily by an inability to perceive physical pain from birth, resulting in the accumulation of bruising, inflammation and fractures that affect patient's life expectancy. In Palestine, because of high rate of consanguinity, this rare disease seems to have higher frequency than in other communities. However, there were no systematic studies to address the genetic factors that cause CIP in the Palestinian community. In this study, we genotyped members of five Palestinian CIP-affected families using Sanger and Whole exome sequencing approaches. Our results confirmed the presence of the founder mutation c.1931-ins- T in the NTRK1 gene of Palestinian Bedouin CIPA patients. This mutation was found in three out of the five participating families. In addition, in one CIPA family, we found the missense mutation (c.2170 G > A (G724 S) in exon 16 of NTRK1 gene. Finally, a novel nonsense mutation (c.901A > T, K301*) was detected in exon 7 of the SCN9A gene in CIP without anhidrosis family. In conclusion our study revealed three mutations that caused CIP, and CIPA in Palestinian community which would help in improving the diagnostic and genetic counseling process. And help in building a diagnostic and follow up protocol for the affected individuals, since early diagnosis and medical care interference could prevent a lot of unpleasant complication of CIP, and CIPA patients.
Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA
Authors: Malnar Mirjana , Čerček Urša , Yin Xiaoke , Ho Manh Tin , Lampret Barbka Repič , Neuman Manuela , Hermann Andreas , Rouleau Guy , Suter Beat , Mayr Manuel , Rogelj Boris 
Published: November 2022

Abstract
The expanded hexanucleotide GGGGCC repeat mutation in the C9orf72 gene is the main genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. Under one disease mechanism, sense and antisense transcripts of the repeat are predicted to bind various RNA-binding proteins, compromise their function and cause cytotoxicity. Focusing on cytoplasmic interaction of the CCCCGG antisense repeat RNA this study identifies phenylalanine-tRNA synthetase (FARS) subunit alpha (FARSA) as the main interactor. The aminoacylation of tRNAPhe by FARS is inhibited by antisense RNA, leading to decreased levels of charged tRNAPhe. Remarkably, this is associated with global reduction of phenylalanine incorporation in the proteome and significant decrease in expression of phenylalanine-rich proteins in cellular models and C9orf72 patient-derived cells. In conclusion, this research reveals functional inhibition of FARSA in the presence of antisense RNA repeats. Compromised aminoacylation of tRNA could lead to impairments in protein synthesis and further contribute to C9orf72 mutation-associated pathology.
Effectiveness of Intra-coronary Injection of Sodium Nitroprusside to The Treatment of Coronary No-Reflow Through Punctured Coronary Balloon
Authors: Lai Changchun , Wang Yunxiang 
Published: November 2022

Abstract
Background
Rapid restoration of TIMI 3 on case of no-reflow during PCI is imperative. Application of punctured coronary balloon intracoronary injection of sodium nitroprusside is a prompt, safe and effective method.
Objective
To investigate the effectiveness of intracoronary injection of Sodium Nitroprusside via punctured coronary balloon in the treatment of no-reflow in coronary artery.Methods: We retrospectively analyzed 76 patients treated with intracoronary injection of Sodium Nitroprusside via punctured coronary balloon for coronary no-reflow.
Results
All 76 patients successfully completed intracoronary injection of Sodium Nitroprusside, with the improvement of no-reflow in coronary artery to TIMI grade III in 74 patients. There were two complications occurred in the early application of this technique: one was a punctured coronary balloon fracture left in the distal coronary artery, and the other was a difficult pullbacking of the punctured coronary balloon catheter. After improving the application method, no more complication case occurred in 67 cases afterward, and no complications such as coronary guide wire pulled out of the coronary artery, coronary artery dissection, coronary artery perforation, and coronary artery air embolism occurred.
Conclusion
Coronary no-reflow treatment with intracoronary injection of Sodium Nitroprusside via punctured coronary balloon is safe and effective when properly applied.
Responses of biomass accumulation and nutrient utilization along a phosphorus supply gradient in Leymus chinensis
Authors: Li Huijun , Liu Gongshe , Sheng Jiandong , Zhang Wentai , Zhao Hongmei , Hu Yutong , Kang Hongliang , Zhou Xiaoguo 
Published: November 2022

Abstract
Phosphorus (P) deficiencies are widespread in calcareous soils. The poor availability of nitrogen (N) and P in soils often restricts crop growth. However, the effects of P addition on plant growth and plant nutrient transport changes during the establishment of Leymus chinensis fields in Xinjiang are not clear. We investigated the responses of Leymus chinensis biomass and nutrient absorption and utilization to changes in soil N and P by adding P (0, 15.3, 30.6, and 45.9 kg ha-1 year-1) with basally applied N fertilizer (150 kg ha-1 year-1). The results showed that (a) Principal component analysis (PCA) of biomass, nutrient accumulation, soil available P, and soil available N during the different periods of Leymus chinensis growth showed that their cumulative contributions during the jointing and harvest periods reached 95.4% and 88%, respectively. (b) Phosphorus use efficiency (PUE) increased with the increase of P fertilizer gradient and then decreased and the maximum PUE was 13.14% under P2 treatment. The accumulation of biomass and nutrients in Leymus chinensis can be effectively improved by the addition of P fertilizer at P2. Different P additions either moderately promoted or excessively inhibited Leymus chinensis growth and nutrient utilization.