bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular mechanisms leading to ceftolozane/tazobactam resistance in clinical isolates of Pseudomonas aeruginosa from five Latin American countries
Authors: Mojica María F. , De La Cadena Elsa , Ríos Rafael , García-Betancur Juan Carlos , Díaz Lorena , Reyes Jinnethe , Hernández-Gómez Cristhian , Radice Marcela , Gales Ana C. , Castañeda Méndez Paulo , Munita José M. , Pallares Christian José , Martínez José R. W. , Villegas María Virginia 
Published: October 2022

ObjectivesIdentify molecular mechanisms responsible for the in vitro non-susceptibility to ceftolozane/tazobactam (TOL) in a group of 158 clinical isolates of Pseudomonas aeruginosa from five Latin American countries collected before the introduction of TOL into the clinical practice.MethodsClinical isolates of P. aeruginosa (n = 504) were collected between January 2016 and October 2017 from 20 hospitals located in Argentina, Brazil, Chile, Colombia, and Mexico. Minimum inhibitory concentrations (MICs) to TOL were determined by standard broth microdilution and interpreted according to CLSI breakpoints. Initially, production of carbapenemases in TOL non-susceptible isolates was assessed by Rapidec® followed by qPCR to detect blaKPC, blaNDM-1, blaVIM, and blaIMP. Illumina® WGS was performed for isolates in which non-susceptibility to TOL was not mediated by carbapenemases.ResultsA total of 158 (31.3%) isolates were non-susceptible to TOL. In 74 (46.8%) of these isolates, non-susceptibility to TOL was explained by the production of at least one carbapenemase. WGS revealed that some isolates carried ESBLs, mutated blaPDC and ampD, associated with decreased susceptibility to TOL.ConclusionSubstitutions found in PDC and carbapenemase production were the most common presumed mechanisms of resistance to TOL detected in this study. This study shows that epidemiological surveillance is warranted to monitor the emergence of novel mechanisms of resistance to TOL that might compromise its clinical utility.
Microbiota independent effects of oligosaccharides on Caco-2 cells -A semi-targeted metabolomics approach using DI-FT-ICR-MS coupled with pathway enrichment analysis
Authors: Jensen-Kroll Julia , Demetrowitsch Tobias , Clawin-Rädecker Ingrid , Klempt Martin , Waschina Silvio , Schwarz Karin 
Published: October 2022

Milk oligosaccharides (MOS) and galactooligosaccharides (GOS) are associated with many benefits, including anti-microbial effects and immune-modulating properties. However, the cellular mechanisms of these are largely unknown. In this study, the effects of enriched GOS and MOS mixtures from caprine and bovine milk consisting mainly 6'-galactosyllactose, 3'-sialyllactose, and 6'-sialyllactose on Caco-2 cells were investigated, and the treatment-specific metabolomes were described. In the control, the cells were treated with a sugar mix consisting of one-third each of glucose, galactose and lactose.A local metabolomics workflow with pathway enrichment was established, which specifically addresses DI-FT-ICR-MS analyses and includes adaptations in terms of measurement technology and sample matrices. By including quality parameters, especially the isotope pattern, we increased the precision of annotation. The independence from online tools, the fast adaptability to changes in databases, and the specific adjustment to the measurement technology and biomaterial used, proved to be a great advantage.For the first time it was possible to find 71 active pathways in a Caco-2 cell experiment. These pathways were assigned to 12 main categories, with amino acid metabolism and carbohydrate metabolism being the most dominant categories in terms of the number of metabolites and metabolic pathways. Treatment of Caco-2 cells with high GOS and glucose contents resulted in significant effects on several metabolic pathways, whereas the MOS containing treatments resulted only for individual metabolites in significant changes. An effect based on bovine or caprine origin alone could not be observed. Thus, it was shown that MOS and GOS containing treatments can exert microbiome-independent effects on the metabolome of Caco-2 cells.
Elevated serum nuclear factor erythroid 2-related factor 2 levels contribute to a poor prognosis after acute supratentorial intracerebral hemorrhage: A prospective cohort study
Authors: Wang Chuan-Liu , Yan Xin-Jiang , Zhang Cheng-Liang , Xu Yan-Wen 
Published: October 2022

ObjectiveNuclear factor erythroid 2-related factor 2 (Nrf2) is a key transcriptional factor for antioxidant response element-regulated genes. The purpose of this study was to assess the prognostic role of serum Nrf2 in intracerebral hemorrhage (ICH).Materials and methodsIn this prospective observational study, serum Nrf2 levels of 115 acute supratentorial ICH patients and 115 controls were gaged. Early neurologic deterioration (END) was defined as an increase of four or greater points in National Institutes of Health Stroke Scale (NIHSS) score or death at post-stroke 24 h. A poor outcome was referred to as the post-stroke 90-day modified Rankin scale (mRS) score of 3–6. END and a poor outcome were considered as the two prognostic parameters.ResultsAs compared to controls, serum Nrf2 levels of patients were substantially elevated (P &lt; 0.001), with its levels increasing during the 6-h period immediately, peaking in 12–18 h, plateauing at 18–24 h, and decreasing gradually thereafter (P &lt; 0.05). Serum Nrf2 levels of patients were independently correlated with NIHSS score (t = 3.033; P = 0.003) and hematoma volume (t = 3.210; P = 0.002), independently predicted END (odds ratio 1.125; 95% confidence interval 1.027–1.232; P = 0.011) and poor outcome (odds ratio 1.217; 95% confidence interval 1.067–1.387; P = 0.013), as well as efficiently distinguished END (area under curve 0.771; 95% confidence interval 0.666–0.877; P &lt; 0.001) and poor outcome (area under curve 0.803; 95% confidence interval 0.725–0.882; P &lt; 0.001). Its predictive ability was equivalent to those of NIHSS score and hematoma volume (both P &gt; 0.05), and it also significantly improved their predictive abilities under receiver operating characteristic (ROC) curve (all P &lt; 0.05).ConclusionElevated serum Nrf2 levels are closely correlated with severity, END, and 90-day poor outcome following ICH. Hence, Nrf2 may play an important role in acute brain injury after ICH, and serum Nrf2 may have the potential to serve as a prognostic biomarker of ICH.
Adjunctive accelerated repetitive transcranial magnetic stimulation for older patients with depression: A systematic review
Authors: Zheng Wei , Zhang Xin-Yang , Xu Rui , Huang Xiong , Zheng Ying-Jun , Huang Xing-Bing , Li Ze-Zhi , Chen Huo-Di 
Published: October 2022

ObjectiveWe performed this systemic review to investigate the therapeutic potential and safety of adjunctive accelerated repetitive transcranial magnetic stimulation (aTMS) for older patients with depression.MethodsWe included published randomized clinical trials (RCTs) and observational studies targeting adjunctive aTMS for older patients with depression.ResultsTwo open-label self-controlled studies (n = 29) fulfilled the criteria for inclusion. The included studies reported significant improvements in depressive symptoms from baseline to post-aTMS (all Ps &lt; 0.05). One study reported a dropout rate of 10.5% (2/19). Mild headache was the most common adverse reaction.ConclusionThe currently available evidence from two open-label self-controlled studies indicates that adjunctive aTMS is a safe and effective therapy for older patients with depression.
Potential value of cerebrospinal fluid α-synuclein in the identification of postoperative delirium undergoing knee/hip arthroplasty: The perioperative neurocognitive disorder and biomarker lifestyle study
Authors: Lin Xu , Guo Yuwei , Dong Rui , Wang Bin , Bi Yanlin 
Published: October 2022

ObjectivePostoperative delirium (POD) is a common postoperative complication, which may be associated with α-synuclein (α-syn). The purpose of this study was to explore the association between the expression level of α-syn in cerebrospinal fluid (CSF) and POD.MethodsWe conducted a prospective observational cohort study, which involved in 740 participants (mean age of 61.86 years, range 40–90 years; 40% female) from the Perioperative Neurocognitive Disorder And Biomarker Lifestyle (PNDABLE) study in the final analysis. POD was diagnosed using the Confusion Assessment Scale (CAM), and its severity was measured using the Memorial Delirium Assessment Scale (MDAS). Enzyme-linked immune-sorbent assay (ELISA) was used to detect the concentrations of α-syn, Aβ40, Aβ42, T-tau, and P-tau in CSF.ResultsThe incidence of POD was 11.22% (83/740). The logistic regression analysis showed that the increased concentrations of CSF α-syn (OR = 1.005, 95%CI 1.004–1.006, P &lt; 0.001), P-tau (OR = 1.093, 95%CI 1.071–1.115, P &lt; 0.001), and T-tau (OR = 1.008, 95%CI 1.006–1.009, P &lt; 0.001) were risk factors of POD. Linear regression showed that CSF α-syn had positive correlations with P-tau (β = 0.480, P &lt; 0.001), T-tau (β = 0.334, P &lt; 0.001), while negative correlations with Aβ40 (β = –0.378 P &lt; 0.001), Aβ42 (β = -0.800, P = 0.001) in POD patients. Mediation analyses showed the association between α-syn and POD was partially mediated by tau pathologies (proportion: 16–17%).ConclusionCSF α-syn is one of the preoperative risk factors for POD, which may be mediated through tau pathologies.Clinical trial registration[www.ClinicalTrials.gov], identifier [ChiCTR20 00033439].
Preoperative computed tomography-determined sarcopenia is a reliable prognostic factor in patients with gastric cancer after radical gastrectomy: A sex-specific analysis
Authors: Liu Ting , Yi Xiaoping , Ge Jie , Zhang Jinwei , Tan Fengbo , Song Kun , Liu Heli , Tang Mimi 
Published: October 2022

BackgroundThe predictive role of sarcopenia in cancer prognosis is an area of increasing concern. However, the influence of sex difference on the predictive role of sarcopenia in cancer prognosis has not been clearly defined. This retrospective cohort study investigated the effect of preoperative sarcopenia on the long-term outcomes of patients with gastric cancer (GC) based on sexual dimorphism.MethodsPreoperative abdominal computed tomography (CT) scans from 379 GC patients who underwent radical gastrectomy were carefully analyzed. The patients were categorized into sarcopenia and non-sarcopenia groups according to the L3 skeletal muscle index (L3 SMI) measured on CT scans. Moreover, other indexes which can be used to evaluate the muscle area or the muscle quality, including skeletal muscle area (SMA), visceral fat area (VFA), subcutaneous fat area (SFA), skeletal muscle radiation attenuation (SM-RA), visceral fat index (VFI), subcutaneous fat index (SFI), and subcutaneous and visceral ratio (SV), were obtained from CT scans.ResultsThere were 254 men and 125 women included in our study. After calculation, we defined sex-specific SMI-related mortality cutoff as 39.73 and 32.97 cm2/m2 for men and women. Univariable analysis showed that pathological tumor-node-metastasis (pTNM), depth of invasion, lymph node metastasis, differentiation degree, preoperative sarcopenia (for men), SMA (for men), L3 SMI, SFA (for women), SFI (for women), SV (for women), and SM-RA (especially for men) were significant independent predictors of overall survival (OS). Multivariable analysis showed that pTNM, depth of invasion, poor differentiation, and SM-RA were significantly associated with 5-year OS in GC patients. However, CT-determined sarcopenia was associated with significantly worse OS only in men, and SFA was significantly associated with 5-year OS only in women.ConclusionSM-RA is a reliable prognostic factor in patients with GC after radical gastrectomy. The impact of indexes mentioned above on survival outcomes is dependent on sex. CT-determined preoperative sarcopenia, a muscle-related indicator, was associated with outcomes in men. Adipose-related indicator (SFA), instead, was associated with outcomes in women.
Endometrial clear cell carcinoma: A population-based study
Authors: Cui Pengfei , Cong Xiaofeng , Zhang Youhao , Zhang Huimin , Liu Ziling 
Published: October 2022

BackgroundA systematic analysis of prognostic factors concerning endometrial clear cell carcinoma (ECCC) is lacking. The current study aimed to construct nomograms predicting the overall survival (OS) of ECCC patients.MethodsWe performed a retrospective study, and predicted nomograms for 3-, 5-, and 10-year OS were established. The nomograms were verified with the consistency index (C-index), calibration curve, and decision curve analysis (DCA).ResultsA total of 1778 ECCC patients, 991 from FIGO stage I/II and 787 from FIGO stage III/IV, were included in this study. The age at diagnosis, marital status, T stage, tumor size, and surgery-independent prognostic factors in FIGO stage I/II, and the age at diagnosis, T stage, lymph node involvement, distant metastasis, tumor size, surgery, radiotherapy, and chemotherapy in FIGO stage III/IV were independent prognostic factors. The C-indexes of the training and validation group were 0.766 and 0.697 for FIGO stage I/II and 0.721 and 0.708 for FIGO stage III/IV, respectively. The calibration curve revealed good agreement between nomogram-predicted and actual observation values. The DCA established that nomograms had better clinical benefits than the traditional FIGO stage.ConclusionsThe predicted nomograms showed good accuracy, excellent discrimination ability, and clinical benefits, depicting their usage in clinical practice.
Case Report: Recurrent pericardial tamponade in a child with COVID-19
Authors: Győri Ádám , Decsi Tamás , Stankovics József , Nyul Zoltán , Környei Mária , Masszi György , Leibinger Evelin , Mosdósi Bernadett 
Published: October 2022

BackgroundPericarditis is rare in Coronavirus disease 2019 (Covid-19) infection and only a few cases were reported in children.Case presentationWe present the case of a 15-year-old boy with symptoms of high fever and worsening chest pain during COVID-19 infection. Chest computer tomography (CT) and echocardiography confirmed pericardial tamponade requiring urgent drainage. Despite antiviral drug treatment, after 18 days severe attack developed requiring repeated pericardiocentesis. High dose ibuprofen, colchicin and the interleukin-1 antagonist, anakinra were given. Clinical symptoms and laboratory parameters improved after seven days of treatment. Autoinflammatory diseases were also suspected in the background the severe pericarditis, but genetic analysis ruled out any mutations.ConclusionPericarditis associated with COVID-19 infection may present in the acute phase or later as MIS-C. Though pericardial tamponade related to ongoing Covid-19 infection is rare in children, even biological treatment with interleukin-1 antagonist may be needed to control the inflammation.
Knowledge and practices related to antibiotic use among women in Malang, Indonesia
Authors: Yunita Sendi Lia , Yang Hui-Wen , Chen Yi-Chun , Kao Li-Ting , Lu Yi-Zi , Wen Yuan-Liang , To Sheng-Yin , Huang Ya-Li 
Published: October 2022

Background:Antimicrobial resistance is a public health problem that threatens the efficacy of antibiotics. Incorrect knowledge of antibiotics may lead to their inappropriate use, hinder their effectiveness, and cause antibiotic resistance. Population-based educational campaigns have been found to have either mixed or no effect on improving knowledge and appropriate antibiotic practices, suggesting the need for more targeted approaches in tailoring education for specific subpopulations. Women are the primary caregivers of their families and are more willing to contact healthcare providers. They had greater knowledge of antibiotics and better adherence to the completion of the antibiotic regimen. Therefore, they are suitable for prioritization in a campaign program.Objective:This study examined the knowledge and practices of female visitors to health centers in Malang, Indonesia with respect to antibiotic use.Methods:This cross-sectional study was conducted in Malang, Indonesia, in July and August 2018. Data were collected from 677 women. Multivariate logistic regression was performed to identify the potential factors associated with antibiotic knowledge, self-medication, and completion of antibiotic regimens.Results:Overall, 82.7% of respondents were aware that antibiotics are used against bacteria, while 38.4% reported self-medication with antibiotics and 51.7% reported completing antibiotic regimens. Women with higher education, previous antibiotic use experience, and very easy accessibility to primary doctors were more likely to have high antibiotic knowledge than those with primary education, no antibiotic use in the previous year, and easy/other level of accessibility to primary doctors. Subjects residing in urban areas and with less accessibility to primary doctors were more likely to self-medicate with antibiotics. Additionally, the completion of antibiotic regimens was positively associated with access to a primary care doctor and high antibiotic knowledge.Conclusion:IF Policymakers tend to reduce inappropriate antibiotic use among women. Priority advocates are recommended for urban residents who have experiences of antibiotic use in the previous year. It is therefore important to increase their awareness, particularly regarding diseases against which antibiotics are effective, and activities such as unnecessary use of antibiotics in healthy animals, which may affect their overall effectiveness among humans. More communication channels should be included in the overall scheme to improve the public awareness and accessibility of health professionals.
Effect of sulfasalazine on endothelium-dependent vascular response by the activation of Nrf2 signalling pathway
Authors: Sonmez Muhammed Ikbal , Shahzadi Andleeb , Kose Cagla , Sonmez Haktan , Ozyazgan Sibel , Akkan Ahmet Gokhan 
Published: October 2022

Background: Diabetes mellitus leads to endothelial dysfunction and accumulation of oxygen radicals. Sulfasalazine-induced Nrf2 activation reduces oxidative stress in vessels. Thus, in the present study, we investigated the effects of sulfasalazine on endothelial dysfunction induced by high glucose. We also ascribed the underlying mechanism involved in glucose-induced endothelial dysfunction.Methods: For this experiment we used 80 Wistar Albino rats thoracic aorta to calculate the dose response curve of noradrenaline and acetylcholine. Vessels were incubated in normal and high glucose for 2 h. To investigate glucose and sulfasalazine effects the vessels of the high glucose group were pre-treated with sulfasalazine (300 mM), JNK inhibitor (SP600125), and ERK inhibitor (U0126) for 30 min. The dose response curve was calculated through organ bath. The eNOS, TAS, TOS, and HO-1 levels were estimated by commercially available ELISA kits.Results: In the high glucose group, the Emax for contraction was significantly higher (p &lt; 0.001), and Emax for relaxation was lower than that of control. These functional changes were parallel with the low levels of eNOS (p &lt; 0.05). High glucose vessel treated with sulfasalazine showed low Emax value for contraction (p &lt; 0.001) however, the Emax for relaxation was significantly high (p &lt; 0.001) when compared to high glucose group. In the JNK group, Emax for contraction and relaxation was inhibited (p &lt; 0.001) compared to sulfasalazine treated vessels. HO—1 enzyme levels were significantly low (p &lt; 0.01) with sulfasalazine but higher with ERK inhibitor (p &lt; 0.05).Conclusion: High glucose induced endothelial dysfunction and sulfasalazine reduced damage in high glucose vessels by activating eNOS, antioxidant effect through HO-1 enzymes and particularly inducing Nrf2 via the ERK and JNK pathways.
Laser projector method for measuring postoperative acetabular anteversion after total hip replacement
Authors: Chen Wei-Cheng , Wu Tai-Yin , Chi Kuan-Yu , Weng Pei-Wei , Huang Yu-Min , Lin Yi-Chia , Liaw Chen-Kun 
Published: October 2022

IntroductionFor patients undergoing THR, measuring the postoperative acetabular anteversion precisely plays a pivotal role in the prognosis. However, using elliptical methods mandates computerized equipment that is frequently in shortage in remote areas and developing countries. We invented a laser projector utilizing the ellipse method to measure the acetabular anteversion directly. The aim is to examine the consistency and validity of the laser projector as compared to our original software, Elliversion.Materials and MethodsWe retrospectively collected 50 postoperative pelvis radiographs including acetabulum from our institution. One investigator first measured the anteversion of included radiographs through Elliversion software as the control group. Subsequently, two operators independently used the laser projector for measurements in two separate periods with 1-day intervals as the experimental group. Our analysis was comprised of intra- and inter-observer comparisons and reliability, which investigated both the consistency and validity, by using two-sample student's t-test and intraclass correlation coefficient.ResultsThere was no significant difference in measuring the anteversion through laser projectors between two operators (p = 0.54), with excellent inter-observer reliability (ICC, 0.967). The estimated effect in the anteversion measurement between the Elliversion and laser projector was also comparable, with the ICC level of 0.984, indicating excellent reliability.ConclusionOur study reported the consistency and validity of this laser projector as there is no significant difference between Elliversion and Laser projector, notably with excellent intra- and inter-observer reliability. We look forward to helping elevate clinical acumen when doctors provide care to patients after THR, especially in remote areas.
PdPty/V2O5-TiO2: Highly Active Catalysts with Good Moisture- and Sulfur Dioxide-Resistant Performance in Toluene Oxidation
Authors: Sun Jingjing , Liu Yuxi , Deng Jiguang , Jing Lin , Bao Minming , Sun Qinpei , Li Linlin , Wu Linke , Hao Xiuqing , Dai Hongxing 
Published: October 2022

Catalytic performance and moisture and sulfur dioxide resistance are important for a catalyst used for the oxidation of volatile organic compounds (VOCs). Supported noble metals are active for VOC oxidation, but they are easily deactivated by water and sulfur dioxide. Hence, it is highly desired to develop a catalyst with high performance and good moisture and sulfur dioxide resistance in the oxidation of VOCs. In this work, we first adopted the hydrothermal method to synthesize a V2O5-TiO2 composite support, and then employed the polyvinyl alcohol (PVA)-protecting NaBH4 reduction strategy to fabricate xPdPty/V2O5-TiO2 catalysts (x and y are the PdPty loading (0.41, 0.46, and 0.49 wt%) and Pt/Pd molar ratio (2.10, 0.85, and 0.44), respectively; the corresponding catalysts are denoted as 0.46PdPt2.10/V2O5-TiO2, 0.41PdPt0.85/V2O5-TiO2, and 0.49PdPt0.44/V2O5-TiO2). Among all the samples, 0.46PdPt2.10/V2O5-TiO2 exhibited the best catalytic activity for toluene oxidation (T50% = 220 °C and T90% = 245 °C at a space velocity of 40,000 mL/(g h), apparent activation energy (Ea) = 45 kJ/mol), specific reaction rate at 230 °C = 98.6 μmol/(gPt s), and turnover frequency (TOFNoble metal) at 230 °C = 142.2 × 10−3 s−1. The good catalytic performance of 0.46PdPt2.10/V2O5-TiO2 was associated with its well-dispersed PdPt2.10 nanoparticles, high adsorbed oxygen species concentration, good redox ability, large toluene adsorption capacity, and strong interaction between PdPty and V2O5-TiO2. No significant changes in toluene conversion were detected when 5.0 vol% H2O or 50 ppm SO2 was introduced to the reaction system. According to the characterization results, we can realize that vanadium is the main site for SO2 adsorption while PdO is the secondary site for SO2 adsorption, which protects the active Pt site from being poisoned by SO2, thus making the 0.46PdPt2.10/V2O5TiO2 catalyst show good sulfur dioxide resistance.