bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial transcriptomic analysis of childhood ependymoma implicates unresolved wound healing as a driver of tumor progression
Authors: Fu Rui , Norris Gregory A. , Willard Nicholas , Griesinger Andrea M. , Riemondy Kent A. , Amani Vladimir , Grimaldo Enrique , Harris Faith , Hankinson Todd C. , Mitra Siddhartha , Ritzmann Timothy A. , Grundy Richard R. , Foreman Nicholas K. , Donson Andrew M. 
Published: March 2022

AbstractChildhood ependymoma (EPN) is a brain tumor that has seen limited improvements in outcome over past decades. The underlying cellular components of EPN have recently been revealed by single cell RNA-sequencing (scRNAseq), providing biological insights. Here we use spatial transcriptomics to comprehensively chart gene expression across the cellular landscape of posterior fossa subgroup A (PFA) EPN, the commonest and most deadly EPN variant, providing novel resolution of cellular heterogeneity and cellular interaction. We reveal that PFA are comprised of epithelial and mesenchymal histological zones each containing a diversity of cellular states. These include co-existing and spatially distinct undifferentiated progenitor-like clusters - a quiescent mesenchymal zone population, and a second highly mitotic progenitor population that is restricted to hypercellular epithelial zones. We show that myeloid cell interaction is the leading cause of mesenchymal transition in PFA, occurring in zones spatially distinct from hypoxia-induced mesenchymal transition, and these distinct EMT-initiating processes were replicated in in-vitro models of PFA. Collectively, our transcriptomic and functional analyses mirror the processes of normal wound healing where PFA mesenchymal and epithelial zones interact with immune subpopulations in cycle of persistent tissue damage response and mitogenic re-epithelialization signals. Spatial transcriptomics advances our understanding of PFA biology, implicating a “wound that will not heal” process as a driver of tumor progression, a new concept that could provide novel targets for effective therapeutic intervention.SignificanceSpatial transcriptomic analysis of the ependymoma tumor microenvironment identifies novel cell populations and interactions, implicating unresolved wound healing as a clinically actionable driver of tumor progression in this refractory childhood brain tumor.
Correlative SIP-FISH-Raman-SEM-NanoSIMS links identity, morphology, biochemistry, and physiology of environmental microbes
Authors: Schaible George A. , Kohtz Anthony J. , Cliff John , Hatzenpichler Roland 
Published: March 2022

AbstractMicroscopic and spectroscopic techniques are commonly applied to study microbial cells but are typically used on separate samples, resulting in population-level datasets that are integrated across different cells with little spatial resolution. To address this shortcoming, we developed a workflow that correlates several microscopic and spectroscopic techniques to generate an in-depth analysis of individual cells. By combining stable isotope probing (SIP), fluorescence in situ hybridization (FISH), scanning electron microscopy (SEM), confocal Raman microspectroscopy (Raman), and nano-scale secondary ion mass spectrometry (NanoSIMS), we illustrate how individual cells can be thoroughly interrogated to obtain information about their taxonomic identity, structure, physiology, and metabolic activity. Analysis of an artificial microbial community demonstrated that our correlative approach was able to resolve the activity of single cells using heavy water SIP in conjunction with Raman and/or NanoSIMS and establish their taxonomy and morphology using FISH and SEM. This workflow was then applied to a sample of yet uncultured multicellular magnetotactic bacteria (MMB). In addition to establishing their identity and activity, backscatter electron microscopy (BSE), NanoSIMS, and energy-dispersive X-ray spectroscopy (EDS) were employed to characterize the magnetosomes within the cells. By integrating these techniques, we demonstrate a cohesive approach to thoroughly study environmental microbes on a single cell level.
The Codon Statistics Database: a Database of Codon Usage Bias
Authors: Subramanian Krishnamurthy , Payne Bryan , Feyertag Felix , Alvarez-Ponce David 
Published: March 2022

ABSTRACTMotivationMost amino acids can be encoded by a set of synonymous codons. Often, for any given amino acid, certain codons are significantly more used than others, a phenomenon known as codon usage bias. The genomes of different species differ in the frequencies at which they use each codon (e.g., a codon that is highly used in one species may be lowly used in another species). In addition, within any given genome, genes differ in their degree of codon bias, with highly expressed genes being more likely to use preferred codons. Knowing the codons that are preferred by a certain genome, and the amount of codon bias exhibited by each gene, has multiple applications (e.g., in heterologous expression, gene prediction, or phylogenetic inference).ResultsWe have developed the Codon Statistics Database, an online database that contains codon usage statistics for all the species with reference or representative genomes in RefSeq. The user can search for any species and access two sets of tables. One set lists, for each codon, the frequency, the Relative Synonymous Codon Usage (RSCU), and whether the codon is preferred. Another set of tables lists, for each gene, its GC content, Effective Number of Codons (ENC), Codon Adaptation Index (CAI), and frequency of optimal codons (Fop). Equivalent tables can be accessed for 1) all nuclear genes, 2) nuclear genes encoding ribosomal proteins, 3) mitochondrial genes and 4) chloroplastic genes (if available in the relevant assembly). The user can also search for any taxonomic group (e.g., “primates”) and obtain a table comparing all the species in the group.AvailabilityThe database is free to access without registration at http://codonstatsdb.unr.edu.
Effects of taurine in mice and zebrafish behavioral assays with translational relevance to schizophrenia
Authors: Giongo Franciele Kich , Gallas-Lopes Matheus , Benvenutti Radharani , Sachett Adrieli , Bastos Leonardo Marensi , Rosa Adriane Ribeiro , Herrmann Ana Paula 
Published: March 2022

AbstractBackgroundAltered redox state and developmental abnormalities in glutamatergic and GABAergic transmission during development are linked to the behavioral changes associated with schizophrenia. As an amino acid that exerts antioxidant and inhibitory actions in the brain, taurine is a potential candidate to modulate biological targets relevant to this disorder. Here, we investigated in mice and zebrafish assays whether taurine prevents the behavioral changes induced by acute administration of MK-801 (dizocilpine), a glutamate NMDA receptor antagonist.MethodsC57BL/6 mice were intraperitoneally administered with saline or taurine (50, 100 and 200 mg/kg) followed by MK-801 (0.15 mg/kg). Locomotor activity, social interaction and prepulse inhibition of the acoustic startle reflex were then assessed in different sets of animals. Zebrafish were exposed to tank water or taurine (42, 150 and 400 mg/L) followed by MK-801 (5 μM); social interaction and locomotor activity were evaluated in the same test.ResultsMK-801 induced hyperlocomotion and disrupted sensorimotor gating in mice; in zebrafish, it reduced sociability while increased locomotion. Taurine was mostly devoid of effects and did not counteract NMDA antagonism in mice or zebrafish.DiscussionContradicting previous clinical and preclinical data, taurine did not show antipsychotic-like effects in the present study. However, it still warrants consideration as a preventive intervention in animal models of relevance to the prodromal phase of schizophrenia; further studies are thus necessary to evaluate whether and how taurine might benefit patients.
Consumers' perception of suboptimal food: strategies to reduce food waste
Authors: Varese Erica , Cesarani Maria Chiara , Wojnarowska Magdalena 
Published: March 2022

PurposeThe purpose of this paper is to review, through a systematic literature review, the different perspectives from which imperfect, ugly and suboptimal food products lead to food waste, as well as to ascertain which are the already proposed improvements, and to present further solutions to develop more sustainable food production and consumption.Design/methodology/approachA systematic literature review was conducted using PRISMA methodology and searching for the following keywords: “imperfect fruit”, “imperfect food”, “ugly fruit” and “ugly food”; the review was carried out within three databases (Emerald, Scopus and Science Direct-Elsevier), starting, since the topic is quite recent, from the first published article on this issue. Only peer-reviewed papers were selected.FindingsResults reveal that, among 188 papers, only 49 fit the aim of the literature review, analysing what strategies can be used to ameliorate consumers' perception of suboptimal food in order to fight food waste. Specifically, four different interventions are suggested: on the supply chain, on personal experience and awareness campaigns, on promoting circular economy, and on marketing and advertising campaigns.Originality/valueThis paper fills a gap in literature since, by the authors' knowledge, this is the first literature review which analyses different strategies on food waste reduction linked to imperfect/suboptimal food, suggesting further steps which may be taken.
Determinants of eXtensible business reporting language adoption: an institutional perspective
Authors: Borgi Hela , Tawiah Vincent 
Published: March 2022

Purpose
This paper aims to examine the institutional factors that influence the adoption of eXtensible Business Reporting Language (XBRL) at the country level.

Design/methodology/approach
The authors use a large sample of 175 developed and developing countries over 14 years. Data is obtained from different sources including, World Development Indicators, the Reports on the Observance of Standards and Codes (ROSC) website and the Quality of Government database.

Findings
The results highlight the significance of coercive, mimetic and normative pressures in terms of ROSC reports, the extent of accounting globalisation and education. However, in further analyses, the authors found that coercive pressure is pronounced in developing countries. Nevertheless, mimetic pressure is an important, influential factor for all countries regardless of their status as developed or developing.

Originality/value
This study responds to the lack of research on the country-level factors of countries’ adoption of XBRL. The present study contributes to the literature by providing additional evidence on the country-level factors influencing XBRL adoption. Using the institutional theory, the authors provide a better understanding of the global diffusion of XBRL, which has attracted little attention. The study also complements prior literature on the adoption of international accounting and financial reporting practices.
Factors influencing green purchase behavior among millennials: the moderating role of religious values
Authors: Qureshi Muhammad Asif , Khaskheli Asadullah , Qureshi Jawaid Ahmed , Raza Syed Ali , Khan Komal Akram 
Published: March 2022

PurposeAn individual’s standard of living is profoundly affected by industrialization and technology’s continuous revolution. At present, the environment is uncontrollable and global warming is increasing. Therefore, there is a need to protect the earth immediately as the lives of all creatures are at risk. The purpose of this paper is to determine the factors related to green purchase behavior (GPB) by incorporating religious values (RGV) as a moderator. Moreover, the moderating role of RGV has been incorporated so that the importance of RGV in the life of millennials can be examined.Design/methodology/approachSmart partial least square (PLS) has been used for data analysis, and PLS-structural equation modeling has been used to assess measurement and structural models.FindingsThe findings reveal that environmental concern, environmental knowledge and green perceived value positively and significantly affect attitude and subjective norm (SN). Moreover, attitudes toward the purchase of green products and SNs also show a positive and significant relationship with green purchase intention (GPI). In addition, GPI is also positively and significantly associated with GPB. However, green brand knowledge portrays a positive but insignificant relationship with attitude and SN. Finally, RGV does not strengthen the relationship between intention and behavior.Originality/valueReligion is a strong predictor of individual behavior as people are emotionally connected with Islam’s teachings. Therefore, the study provides a unique contribution by adding RGV as a moderator in the model of TRA. Also, the authors targeted the specific generation, i.e. millennials, so that millennials’ behavior can be identified as it covers Pakistan’s large population. Also, millennials are the people who are more involved in decision-making.
Pricing design and contract selection with customers’ self-control: a strategic analysis
Authors: Yuwei Gao , Chen Yuan , Zhu Yangguang , Du Shaofu 
Published: March 2022

Purpose
The purpose of this paper is to examine how customers’ self-control affects their purchase decisions and to discuss the pricing decisions of the retailer under different forms of contract.

Design/methodology/approach
The authors use the literature on hyperbolic discounting to model customers’ self-control problems. In this framework, the authors examine how the customers’ self-control affects the optimal pricing decision and the selection of the optimal contract form when there is a supplier and a retailer in the supply chain.

Findings
The study’s results show that when wholesale price contract is compared with buyback contract, buyback contract is better when customers’ self-control is weak; when quantity-discount contract is compared with wholesale price contract and buyback contract, although quantity discount can encourage customers to purchase more units of products, but both wholesale price contract and buyback contract can be better than quantity-discount contract in some cases. Additionally, the authors demonstrate that revenue sharing contract can increase the supply chain’s profit. The authors also find that sometimes customers’ preplan will lead to the result that the supplier produces more unhealthy products.

Originality/value
To the best of the authors’ knowledge, this is the first study to analyze the decision-making of the retailer by developing an analytical framework combining customer’s self-control and supply chain contract. These results have important implications for the supplier and the retailer that sell vice goods.
Creating immersive and parasocial live shopping experience for viewers: the role of streamers' interactional communication style
Authors: Liao Junyun , Chen Keyi , Qi Jun , Li Ji , Yu Irina Y. 
Published: March 2022

PurposeLive streaming commerce has grown rapidly in recent years. Although the way streamers communicate with viewers is the key to influencing users' purchase behaviors, little is known about the influence of streamers' communication style on purchase intention. Drawing on parasocial interaction theory and flow theory, this study examines the influence of streamers' interaction orientation on viewers' immersion and parasocial interactions, and its effect on viewers' purchase intentions. The moderating effect of streamers' expertise and attractiveness is also examined.Design/methodology/approachThe authors conducted an empirical study using a structural equation modeling approach based on 407 responses to a questionnaire completed by live streaming audiences.FindingsResults indicate that streamers' interaction orientation has a positive effect on viewers' immersion and parasocial interactions, in turn positively affecting viewers' willingness to purchase. Streamers' expertise positively moderates the effect of their interaction orientation on viewers' immersion and parasocial interactions.Originality/valueThis research is among the first to explore the effect of streamers' communication style and provides a framework for how their communication style affects viewers' purchase intention by enhancing immersion and parasocial interaction in live streaming commerce.
Police encounters with individuals perceived as having intellectual or developmental disabilities: an examination of encounter characteristics from two studies
Authors: Watson Amy , Phan Megan , Compton Michael T. 
Published: March 2022

PurposeVery little is known about the frequency and nature of police contacts with individuals with intellectual or developmental disabilities (I/DDs). The purpose of the study is to examine the characteristics of police contacts with persons with I/DD and how they differ from other behavioral health-related encounters.Design/methodology/approachThe authors draw on data from two large National Institute of Mental Health (NIMH)-funded studies of police response to mental/behavioral health crises and the Crisis Intervention Team (CIT) approach. Both projects used the same incident report form. The authors examine detailed information from 219 calls in which officers perceived that the subject had I/DD, either alone or co-occurring with a mental illness or substance use disorder and compared them with calls in which I/DD was not indicated (n = 1,305).FindingsCalls with subjects with I/DD most frequently occurr ed in homes or on the street and were resolved without formal action. The most frequent subject symptoms/behaviors noted were confusion and anxiety. Some differences from calls with individuals without I/DD were noted in term of presentation and outcomes.Research limitations/implicationsData relied on officer perception of subject having I/DD, which may miss more subtle indicators.Social implicationsThere is a need for specific research attention to police contacts with persons with I/DD that differentiates those contacts from other behavioral health-related encounters.Originality/valueThere is still much the authors do not know about police contacts with persons with I/DD. However, findings provide a preliminary glimpse into the nature of these contacts and suggest they may differ somewhat from other behavioral health related police encounters.
Work-life balance satisfaction in crisis times: from luxury to necessity – The role of organization's responses during COVID-19 lockdown
Authors: Ortiz-Bonnin Silvia , Blahopoulou Joanna , García-Buades M. Esther , Montañez-Juan Maribel 
Published: March 2022

PurposeThis study examines how satisfaction with work-life balance (WLB) in combination with satisfaction with organization's COVID-19 responses (SOCV19R) helps to enhance subjective well-being and performance during the lockdown due to COVID-19.Design/methodology/approachThe data of this time-lagged study were gathered through an online survey with three-waves between March and May 2020 in Spain (N = 167). Hierarchical multiple regression and PROCESS were used to test the hypotheses.FindingsDirect relationships between SOCV19R and subjective well-being and performance were not significant. Instead, SOCV19R increased employees' well-being and performance through a higher satisfaction with WLB (full mediation).Originality/valueThe novelty of this study is the evaluation of SOCV19R as a form of organizational support in times of crisis. This study suggests that a good organizational reaction to face a crisis such as the pandemic, encourages employees' WLB and helps them to boost their well-being and performance. It may be concluded that work-life balance (WLB) in Spain was seen as a luxury in good times and turned out to be a necessity in bad times. The present study recommends practical implications and provides lessons for human resource management for future crises or similar work conditions.
Classifying elders neglect, insult and abuse through financial hardship and physical health
Authors: Bajpai Naval , Kulshreshtha Kushagra , Dubey Prince , Sharma Gunjan 
Published: March 2022

PurposeAging has detrimental effects on elders due to their physical health and financial hardship. Elders face neglect, insult and abuse in society due to causes related to physical health and financial issue from caregivers. This study aims to identify the measures of physical health and financial hardship and classifies elders under neglect, insult and abuse categories.Design/methodology/approachThe propositions of existence and classifying elders under neglect, insult and abuse categories were tested by using discriminant analysis and their profiling was done by perceptual mapping technique.FindingsThe elder neglect category identified as a prominent category due to physical health while elder insult and abuse were caused by physical health and financial hardship both. The present study portrays the multi-dimensional facets related to elders’ ill-treatment. The elder’s ill-treatment categories were profiled to imply the measure of elevating elders’ dignity and care at a personal level and society at large.Originality/valueThis study classifies elders under neglect, insult and abuse categories. This classification may facilitate the medical practitioners, academicians and government and non-government social welfare agencies in understanding elder abuse with new perspectives.
Mushroom‐shaped structures formed in Acinetobacter baumannii biofilms grown in a roller bioreactor are associated with quorum sensing–dependent Csu‐pilus assembly
Authors: Romero Manuel , Mayer Celia , Heeb Stephan , Wattanavaekin Krittanont , Cámara Miguel , Otero Ana , Williams Paul 
Published: March 2022

SummaryThere is currently a need to develop simple biofilm models that facilitate investigation of the architecture/biology of mature bacterial biofilms in a consistent/standardized manner given their environmental and clinical importance and the need for new anti‐biofilm interventions. This study introduces a novel biofilm culture system termed the rolling biofilm bioreactor (RBB). This easily operated system allows adherent microbial cells to be repeatedly exposed to air/solid/liquid interfaces optimizing biofilm growth. The RBB was exploited to investigate biofilm formation in Acinetobacter baumannii. High levels of A. baumannii biofilm biomass reproducibly accumulate in the RBB and, importantly, undergo a maturation step to form large mushroom‐shaped structures that had not been observed in other models. Based on image analysis of biofilm development and genetic manipulation, we show how N‐acylhomoserine lactone‐dependent quorum sensing (QS) impacts on biofilm differentiation, composition and antibiotic tolerance. Our results indicate that extracellular DNA (eDNA) is a key matrix component in mature Acinetobacter biofilms as the mushroom‐like structures consist of dense cellular masses encased in an eDNA mesh. Moreover, this study reveals the contribution of QS to A. baumannii biofilm differentiation through Csu pilus assembly regulation. Understanding the mechanisms of structural development of mature biofilms helps to identify new biofilm eradication and removal strategies.