bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of the Ryukyu Current to Climate Change During 1993–2018: Is There a Robust Trend?
Authors: Liu Zhao‐Jun , Zhu Xiao‐Hua , Nakamura Hirohiko , Wang Min , Nishina Ayako , Qiao Yu‐Xiang , Zhu Ze‐Nan 
Published: December 2022

AbstractLinear trends in the Ryukyu Current, a part of the western boundary current in the western North Pacific flowing on the seaside of the Ryukyu Island chain, were investigated using reanalysis data during 1993–2018. The subsurface Ryukyu Current has weakened along its path during the recent decades. Two determinant factors for the weakened subsurface Ryukyu Current are as follows. First, the first baroclinic topographic Rossby wave propagated signals emanating from the Tokara Strait (TK) and the Kerama Gap (KG) southward along the eastern slope of the Ryukyu Island chain, which depressed the onshore side of the isopycnal. The weakened Kuroshio in the TK and weakened overflow through the KG during this period led to isopycnal shoaling along the onshore side east of the Ryukyu Island chain, slowing down the Ryukyu Current. Second, anticyclonic eddies emanated from the interior region near 30°N and 170°W increased during this period. These anticyclonic eddies, translating southwestward, reached east of the Ryukyu Island chain, and finally deepened the offshore side of the isopycnal depth of the Ryukyu Current. The isopycnal across the Ryukyu Current velocity core became less steep, thereby weakening the subsurface Ryukyu Current. Moreover, a positive trend of sea surface height anomaly southeast of Miyakojima, driven by wind stress changes in high latitudes (near the Kuroshio Extension band), strengthened the northward current in the upper layer southeast of Miyakojima.
Wildfires Temperature Estimation by Complementary Use of Hyperspectral PRISMA and Thermal (ECOSTRESS & L8)
Authors: Amici S. , Spiller D. , Ansalone L. , Miller L. 
Published: December 2022

AbstractThis paper deals with detection and temperature analysis and of wildfires using PRISMA imagery. Precursore IperSpettrale della Missione Applicativa (Hyperspectral Precursor of the Application Mission, PRISMA) is a new hyperspectral mission by ASI (Agenzia Spaziale Italiana, Italian Space Agency) launched in 2019. This mission provides hyperspectral images with a spectral range of 400–2,500 nm and an average spectral resolution less than 12 nm and a spatial resolution of 30 m/pixel. This study focuses on the wildfire temperature estimation over the Bootleg Fire, US 2021. The analysis starts by considering the Hyperspectral Fire Detection Index (HFDI) which is used to analyze the informative content of the images, along with the analysis of some specific visible, near‐infrared and shortwave‐infrared bands. This first analysis is used as input to perform a temperature estimation of the areas with active wildfire. Surface temperature is retrieved using PRISMA radiance and a linear mixing model based on two background components (vegetation and burn scar) and two active fire components. PRISMA temperatures are compared with LST (Land Surface Temperature) products from NASA's ECOSTRESS and Landsat 8 which imaged the Bootleg Fire before and after PRISMA. A critical discussion of the results obtained with PRISMA is presented, followed by the advantages and limitation of the proposed approach.
Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing
Authors: Oh Jin-Mi , An Minae , Son Dae-Soon , Choi Jinhyuk , Cho Yong Beom , Yoo Chang Eun , Park Woong-Yang 
Published: December 2022

AbstractSingle-cell ribonucleic acid (RNA) sequencing (scRNA-seq) is an effective technique for estimating the cellular composition and transcriptional profiles of individual cells from fresh tissue. Single-nucleus RNA sequencing (snRNA-seq) is necessary to perform this type of analysis in frozen or difficult-to-dissociate tissues, which cannot be subjected to scRNA-seq. This difference in the state of tissues leads to variation in cell-type distributions among each platform. To identify the characteristics of these methods and their differences, scRNA-seq and snRNA-seq were performed in parallel for colon and liver tissues. The two platforms revealed similar diversity but different proportions of cell types in matched tissues. The proportions of epithelial cells in the colon and hepatocytes in the liver were relatively high in snRNA-seq and that of immune cells was relatively high in scRNA-seq. This difference could be explained by variations in the expression scores of adhesion genes due to the disruption of the cytoplasmic contents during scRNA-seq. The enrichment of epithelial cells in the colon resulted in a discrepancy in the differentiation of epithelial cells. This enrichment was also well matched with the images of hematoxylin and eosin staining and the estimated distribution of cell types in bulk RNA sequencing. These results showed that snRNA-seq could be used to analyze tissues that cannot be subjected to scRNA-seq and provides more information in specific cell type analysis.
Diagnostic accuracy of OCTA and OCT for myopic choroidal neovascularisation: a systematic review and meta-analysis
Authors: Ho Sharon , Ly Angelica , Ohno-Matsui Kyoko , Kalloniatis Michael , Doig Gordon S. 
Published: December 2022

Abstract
Background/Objectives
The purpose of this project was to systematically review and meta-analyse studies assessing the diagnostic accuracy of optical coherence tomography angiography (OCTA) and optical coherence tomography (OCT) for myopic choroidal neovascularisation (mCNV). Fluorescein angiography (FA) was accepted as the reference standard.

Methods
PUBMED and EMBASE were searched from inception to March 2021 for studies evaluating the test accuracy of OCTA and/or OCT for diagnosing mCNV. The Preferred Reporting Items for Systematic Reviews and Meta-analyses of Diagnostic Test Accuracy Studies guideline was followed, and the Grading of Recommendations, Assessment, Development and Evaluation approach was used to frame clinical recommendations. Pooled estimates of test accuracy were obtained using a bivariate model.

Results
Of 410 studies assessed for eligibility, 3 studies were identified that compared OCTA to FA and 3 studies were identified that compared spectral domain (SD) OCT to FA. All studies had at least one major methodological flaw leading to an overall high risk of bias. On meta-analysis, the pooled sensitivity of OCTA was 0.89 (95% CI 0.78–0.94) and pooled specificity was 0.93 (95% CI 0.79–0.98). The pooled sensitivity of SD-OCT was 0.99 (95% CI 0.91–1.00). Due to uncertainty in individual studies, the pooled specificity of SD-OCT could not be estimated.

Conclusions
OCTA can reliably diagnose mCNV in clinically suspected patients, however, SD-OCT may not reliably establish a positive diagnosis of mCNV. Future large, prospective studies with improvements in conduct and reporting are needed to strengthen these clinical recommendations.
BRCA2 associates with MCM10 to suppress PRIMPOL-mediated repriming and single-stranded gap formation after DNA damage
Authors: Kang Zhihua , Fu Pan , Alcivar Allen L. , Fu Haiqing , Redon Christophe , Foo Tzeh Keong , Zuo Yamei , Ye Caiyong , Baxley Ryan , Madireddy Advaitha , Buisson Remi , Bielinsky Anja-Katrin , Zou Lee , Shen Zhiyuan , Aladjem Mirit I. , Xia Bing 
Published: December 2022

AbstractThe BRCA2 tumor suppressor protects genome integrity by promoting homologous recombination-based repair of DNA breaks, stability of stalled DNA replication forks and DNA damage-induced cell cycle checkpoints. BRCA2 deficient cells display the radio-resistant DNA synthesis (RDS) phenotype, however the mechanism has remained elusive. Here we show that cells without BRCA2 are unable to sufficiently restrain DNA replication fork progression after DNA damage, and the underrestrained fork progression is due primarily to Primase-Polymerase (PRIMPOL)-mediated repriming of DNA synthesis downstream of lesions, leaving behind single-stranded DNA gaps. Moreover, we find that BRCA2 associates with the essential DNA replication factor MCM10 and this association suppresses PRIMPOL-mediated repriming and ssDNA gap formation, while having no impact on the stability of stalled replication forks. Our findings establish an important function for BRCA2, provide insights into replication fork control during the DNA damage response, and may have implications in tumor suppression and therapy response.
Manipulation on active electronic states of metastable phase β-NiMoO4 for large current density hydrogen evolution
Authors: Wang Zengyao , Chen Jiyi , Song Erhong , Wang Ning , Dong Juncai , Zhang Xiang , Ajayan Pulickel M. , Yao Wei , Wang Chenfeng , Liu Jianjun , Shen Jianfeng , Ye Mingxin 
Published: December 2022

AbstractNon-noble transition metal oxides are abundant in nature. However, they are widely regarded as catalytically inert for hydrogen evolution reaction (HER) due to their scarce active electronic states near the Fermi-level. How to largely improve the HER activity of these kinds of materials remains a great challenge. Herein, as a proof-of-concept, we design a non-solvent strategy to achieve phosphate substitution and the subsequent crystal phase stabilization of metastable β-NiMoO4. Phosphate substitution is proved to be imperative for the stabilization and activation of β-NiMoO4, which can efficiently generate the active electronic states and promote the intrinsic HER activity. As a result, phosphate substituted β-NiMoO4 exhibits the optimal hydrogen adsorption free energy (−0.046 eV) and ultralow overpotential of −23 mV at 10 mA cm−2 in 1 M KOH for HER. Especially, it maintains long-term stability for 200 h at the large current density of 1000 mA cm−2 with an overpotential of only −210 mV. This work provides a route for activating transition metal oxides for HER by stabilizing the metastable phase with abundant active electronic states.
Dynamic spatiotemporal determinants modulate GPCR:G protein coupling selectivity and promiscuity
Authors: Sandhu Manbir , Cho Aaron , Ma Ning , Mukhaleva Elizaveta , Namkung Yoon , Lee Sangbae , Ghosh Soumadwip , Lee John H. , Gloriam David E. , Laporte Stéphane A. , Babu M. Madan , Vaidehi Nagarajan 
Published: December 2022

AbstractRecent studies have shown that G protein coupled receptors (GPCRs) show selective and promiscuous coupling to different Gα protein subfamilies and yet the mechanisms of the range of coupling preferences remain unclear. Here, we use Molecular Dynamics (MD) simulations on ten GPCR:G protein complexes and show that the location (spatial) and duration (temporal) of intermolecular contacts at the GPCR:Gα protein interface play a critical role in how GPCRs selectively interact with G proteins. We identify that some GPCR:G protein interface contacts are common across Gα subfamilies and others specific to Gα subfamilies. Using large scale data analysis techniques on the MD simulation snapshots we derive aspatio-temporal codefor contacts that confer G protein selective coupling and validated these contacts using G protein activation BRET assays. Our results demonstrate that promiscuous GPCRs show persistent sampling of the common contacts more than G protein specific contacts. These findings suggest that GPCRs maintain contact with G proteins through a common central interface, while the selectivity comes from G protein specific contacts at the periphery of the interface.
Chemoenzymatic synthesis of sulfur-linked sugar polymers as heparanase inhibitors
Authors: He Peng , Zhang Xing , Xia Ke , Green Dixy E. , Baytas Sultan , Xu Yongmei , Pham Truong , Liu Jian , Zhang Fuming , Almond Andrew , Linhardt Robert J. , DeAngelis Paul L. 
Published: December 2022

AbstractComplex carbohydrates (glycans) are major players in all organisms due to their structural, energy, and communication roles. This last essential role involves interacting and/or signaling through a plethora of glycan-binding proteins. The design and synthesis of glycans as potential drug candidates that selectively alter or perturb metabolic processes is challenging. Here we describe the first reported sulfur-linked polysaccharides with potentially altered conformational state(s) that are recalcitrant to digestion by heparanase, an enzyme important in human health and disease. An artificial sugar donor with a sulfhydryl functionality is synthesized and enzymatically incorporated into polysaccharide chains utilizing heparosan synthase. Used alone, this donor adds a single thio-sugar onto the termini of nascent chains. Surprisingly, in chain co-polymerization reactions with a second donor, this thiol-terminated heparosan also serves as an acceptor to form an unnatural thio-glycosidic bond (‘S-link’) between sugar residues in place of a natural ‘O-linked’ bond. S-linked heparan sulfate analogs are not cleaved by human heparanase. Furthermore, the analogs act as competitive inhibitors with > ~200-fold higher potency than expected; as a rationale, molecular dynamic simulations suggest that the S-link polymer conformations mimic aspects of the transition state. Our analogs form the basis for future cancer therapeutics and modulators of protein/sugar interactions.
A bioinspired flexible neuromuscular system based thermal-annealing-free perovskite with passivation
Authors: Liu Jiaqi , Gong Jiangdong , Wei Huanhuan , Li Yameng , Wu Haixia , Jiang Chengpeng , Li Yuelong , Xu Wentao 
Published: December 2022

AbstractBrain-inspired electronics require artificial synapses that have ultra-low energy consumption, high operating speed, and stable flexibility. Here, we demonstrate a flexible artificial synapse that uses a rapidly crystallized perovskite layer at room temperature. The device achieves a series of synaptic functions, including logical operations, temporal and spatial rules, and associative learning. Passivation using phenethyl-ammonium iodide eliminated defects and charge traps to reduce the energy consumption to 13.5 aJ per synaptic event, which is the world record for two-terminal artificial synapses. At this ultralow energy consumption, the device achieves ultrafast response frequency of up to 4.17 MHz; which is orders of magnitude magnitudes higher than previous perovskite artificial synapses. A multi-stimulus accumulative artificial neuromuscular system was then fabricated using the perovskite synapse as a key processing unit to control electrochemical artificial muscles, and realized muscular-fatigue warning. This artificial synapse will have applications in future bio-inspired electronics and neurorobots.
Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation
Authors: Li Wei , Long Qi , Wu Hao , Zhou Yanshuang , Duan Lifan , Yuan Hao , Ding Yingzhe , Huang Yile , Wu Yi , Huang Jinyu , Liu Delong , Chen Baodan , Zhang Jian , Qi Juntao , Du Shiwei , Li Linpeng , Liu Yang , Ruan Zifeng , Liu Zihuang , Liu Zichao , Zhao Yifan , Lu Jianghuan , Wang Junwei , Chan Wai-Yee , Liu Xingguo 
Published: December 2022

AbstractPluripotent stem cells hold great promise in regenerative medicine and developmental biology studies. Mitochondrial metabolites, including tricarboxylic acid (TCA) cycle intermediates, have been reported to play critical roles in pluripotency. Here we show that TCA cycle enzymes including Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha and Mdh2 are translocated to the nucleus during somatic cell reprogramming, primed-to-naive transition and totipotency acquisition. The nuclear-localized TCA cycle enzymes Pdha1, Pcb, Aco2, Cs, Idh3a promote somatic cell reprogramming and primed-to-naive transition. In addition, nuclear-localized TCA cycle enzymes, particularly nuclear-targeted Pdha1, facilitate the 2-cell program in pluripotent stem cells. Mechanistically, nuclear Pdha1 increases the acetyl-CoA and metabolite pool in the nucleus, leading to chromatin remodeling at pluripotency genes by enhancing histone H3 acetylation. Our results reveal an important role of mitochondrial TCA cycle enzymes in the epigenetic regulation of pluripotency that constitutes a mitochondria-to-nucleus retrograde signaling mode in different states of pluripotent acquisition.
Definition of the estrogen negative feedback pathway controlling the GnRH pulse generator in female mice
Authors: McQuillan H. James , Clarkson Jenny , Kauff Alexia , Han Su Young , Yip Siew Hoong , Cheong Isaiah , Porteous Robert , Heather Alison K. , Herbison Allan E. 
Published: December 2022

AbstractThe mechanisms underlying the homeostatic estrogen negative feedback pathway central to mammalian fertility have remained unresolved. Direct measurement of gonadotropin-releasing hormone (GnRH) pulse generator activity in freely behaving mice with GCaMP photometry demonstrated striking estradiol-dependent plasticity in the frequency, duration, amplitude, and profile of pulse generator synchronization events. Mice with Cre-dependent deletion of ESR1 from all kisspeptin neurons exhibited pulse generator activity identical to that of ovariectomized wild-type mice. An in vivo CRISPR-Cas9 approach was used to knockdown ESR1 expression selectively in arcuate nucleus (ARN) kisspeptin neurons. Mice with >80% deletion of ESR1 in ARN kisspeptin neurons exhibited the ovariectomized pattern of GnRH pulse generator activity and high frequency LH pulses but with very low amplitude due to reduced responsiveness of the pituitary. Together, these studies demonstrate that estrogen utilizes ESR1 in ARN kisspeptin neurons to achieve estrogen negative feedback of the GnRH pulse generator in mice.
Photoinduced β-fragmentation of aliphatic alcohol derivatives for forging C–C bonds
Authors: Gao Yiman , Liu Jie , Wei Cong , Li Yan , Zhang Kui , Song Liangliang , Cai Lingchao 
Published: December 2022

AbstractAlcohols are ubiquitous in chemistry and are native functionalities in many natural products and bioactive molecules. As such, a strategy that utilizes hydroxy-containing compounds to develop bond disconnection and bond formation process would achieve molecular diversity. Herein we utilize bench-stable N-alkoxyphthalimides prepared from alcohols to couple with glycine derivatives via radical process under visible light irradiation, providing a variety of unnatural amino acid (UAA) and peptide derivatives. The approach allows to rapidly deconstruct molecular complexity via β-fragmentation such as saclareolide, β-pinene and camphor and provides products with unique scaffolds, which show inhibition toward the pathogenic fungi growth.
Overview of lunar dust toxicity risk
Authors: Pohlen Michael , Carroll Danielle , Prisk G. Kim , Sawyer Aenor J. 
Published: December 2022

AbstractLunar dust (LD), the component of lunar regolith with particle sizes less than 20 μm, covers the surface of the Moon. Due to its fineness, jagged edges, and electrostatic charge, LD adheres to and coats almost any surface it contacts. As a result, LD poses known risks to the proper functioning of electronic and mechanical equipment on the lunar surface. However, its mechanical irritancy and chemical reactivity may also pose serious health risks to humans by a number of mechanisms. While Apollo astronauts reported mild short-lived respiratory symptoms, the spectrum of health effects associated with high-dose acute exposure or chronic low-dose exposure are not yet well-understood. This paper explores known and potential human risks of exposure to LD which are thought to be important in planning upcoming lunar missions and planetary surface work.
Diagnostic and prognostic value of blood inflammation and biochemical indicators for intrahepatic cholestasis of pregnancy in Chinese pregnant women
Authors: Luo Mengjun , Wang Li , Yao Haibo , Wen Yizhou , Cao Dengcheng , Shen Wei , Liu Chenggui 
Published: December 2022

AbstractIntrahepatic cholestasis of pregnancy (ICP) is a common liver disease during pregnancy, that has serious complications. This study aimed to compare the blood inflammation and biochemical markers of pregnant women with ICP in Southwest China and analyse their diagnostic value for ICP. A controlled cross-sectional study was conducted, and routine blood and biochemical indicators of 304 diagnosed ICP patients and 363 healthy pregnant women undergoing routine prenatal examination were assessed. The blood inflammatory indicators and biochemical indicators were compared between the ICP groups and normal groups. In this study, the levels of the ALT, AST, GGT, TBIL and DBIL biochemical indicators and the levels of WBC, neutrophils, NLR and PLR inflammatory indicators in the ICP group were significantly higher than those in healthy pregnant women (p < 0.001). The PA and lymphocytes of the ICP group were significantly lower than those of the normal group (p < 0.001). ROC curves showed that ALT and the NLR had higher predictive value for ICP. The GGT, TBA and NLR of pregnant women with ICP in the preterm group were significantly higher than those in the term group, and the combined NLR and TBA had a certain predictive value for preterm birth.
Genetic epidemiology of inherited retinal diseases in a large patient cohort followed at a single center in Italy
Authors: Karali Marianthi , Testa Francesco , Di Iorio Valentina , Torella Annalaura , Zeuli Roberta , Scarpato Margherita , Romano Francesca , Onore Maria Elena , Pizzo Mariateresa , Melillo Paolo , Brunetti-Pierri Raffaella , Passerini Ilaria , Pelo Elisabetta , Cremers Frans P. M. , Esposito Gabriella , Nigro Vincenzo , Simonelli Francesca , Banfi Sandro 
Published: December 2022

AbstractInherited retinal diseases (IRDs) are the leading cause of vision loss in the working-age population. We performed a retrospective epidemiological study to determine the genetic basis of IRDs in a large Italian cohort (n = 2790) followed at a single referral center. We provided, mainly by next generation sequencing, potentially conclusive molecular diagnosis for 2036 patients (from 1683 unrelated families). We identified a total of 1319 causative sequence variations in 132 genes, including 353 novel variants, and 866 possibly actionable genotypes for therapeutic approaches. ABCA4 was the most frequently mutated gene (n = 535; 26.3% of solved cases), followed by USH2A (n = 228; 11.2%) and RPGR (n = 102; 5.01%). The other 129 genes had a lower contribution to IRD pathogenesis (e.g. CHM 3.5%, RHO 3.5%; MYO7A 3.4%; CRB1 2.7%; RPE65 2%, RP1 1.8%; GUCY2D 1.7%). Seventy-eight genes were mutated in five patients or less. Mitochondrial DNA variants were responsible for 2.1% of cases. Our analysis confirms the complex genetic etiology of IRDs and reveals the high prevalence of ABCA4 and USH2A mutations. This study also uncovers genetic associations with a spectrum of clinical subgroups and highlights a valuable number of cases potentially eligible for clinical trials and, ultimately, for molecular therapies.