bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unconfined compressive strength and pore structure evolution law of structural clay after disturbance
Authors: Tang Bin , Xie Liang , Wang Wei , Zhou Biaohe , Gong Yanxia 
Published: August 2022

The clay in the Zhanjiang Formation has thixotropic properties, which has greatly influenced the foundation engineering in the Zhanjiang area. The evolution law of macroscopic strength and clay microstructure during thixotropy can be used to explain the practical engineering problems caused by thixotropy. For undisturbed and reconstituted soil curing for a different period, unconfined compressive strength test, scanning electron microscopy, and mercury injection porosimetry test were carried out to obtain the unconfined compressive strength and pore structure evolution law in the thixotropic process. The results indicate that the Zhanjiang Formation structural clay is very sensitive to disturbance and its unconfined compressive strength decreases from 180.29 to 11.73 kPa after the natural structure is completely destructed. After 300 d of curing, the unconfined compressive strength of clay increased from 11.73 to 53.43 kPa because of thixotropy, which increased by 3.55 times. The stacking flaky flocculation structure of the undisturbed soil is destructed by reconstituting, turning to flaky flocculation structure, and the large pores are homogenized, the small pores develop into medium pores, and there is a decrease in soil strength. In the process of thixotropy, the soil particles gradually coagulate and form an aggregates flocculation structure, and the strength of clay increases with the increase in the degree of cementation. Based on the results, the thixotropic pattern of clay was established and its thixotropic mechanism was explained.
Fault-bounded models of oil–Gas and gas–Hydrate accumulation in the Chaoshan Depression, the South China Sea
Authors: Guangjian Zhong , Changmao Feng , Yanlin Wang , Shenghong Chen , Ming Sun , Hai Yi , Junhui Yu , Jing Zhao , Zhongquan Zhao 
Published: August 2022

The Dongsha Basin is a large Mesozoic basin extended from the northern South China Sea (SCS) to onshore South China. Though long-term uplift and denudation occurred since the end of the Mesozoic, still thick Mesozoic strata (up to 5,000 m) relict over the Dongsha waters where lies the largest depression, Chaoshan Depression, covering an area of 3.7 × 104 km2. It was confirmed by a drilling hole, the well LF35-1-1, that high organic carbon-bearing marine Jurassic layers are present in the depression. However, due to the complexity of the superposed Mesozoic and Cenozoic tectonism and poor imaging quality in previous surveys, the petroleum geology remains poorly understood in view of the deep basin structure, the Mesozoic hydrocarbon migration conditions, and the oil–gas accumulation mechanism. In recent surveys, the seismic imaging quality has been significantly improved by employing long and quasi-3D seismic streamer techniques. Correlating with the regional geology onshore the South China, drilling data of the Well LF35-1-1, and well-tying seismic profiles, it is found that two sets of source rocks are developed in the semi-closed gulf during the Upper Triassic-Lower Jurassic and the Upper Jurassic. Their effective thicknesses are estimated as 495 m and 600 m, respectively, being hopeful with high hydrocarbon generation potentials. During the Dongsha Movement that occurred in the late Cenozoic, deep faults have been extensively activated to disturb the overlying sequences, even in some places breaking through the seafloor. A potential trap structure, DS-A, is found in an intra-sag bulge which is bounded by antithetic and synthetic faults. The oil and gas generated in the neighboring sags can migrate along the faults into reservoir layers at higher levels. The antithetic faults also play the role of seal for oil and gas from the hanging wall. Apparent flat bright spots appearing within the DS-A trap indicate likely entrapment of layered petroleum. The synthetic faults on the opposite side of the DS-A structure, although fails to seal oil–gas reservoirs, provide plumbing channels for oil and gas to leak to the shallow layers above which a few pockmarks and mud volcanoes are visible. As the water depth of the continental slope there ranges from 300 m to 2000 m, it is likely for the leaked gas to form natural gas hydrates. A close cogenetic interrelation exists between the natural gas hydrates at the seafloor and oil–gas reservoirs in the deep.
Youth’s perception toward ecotourism as a possible model for sustainable use of local tourism resources
Authors: Stanciu Mirela , Popescu Agatha , Sava Camelia , Moise George , Nistoreanu Bogdan Gabriel , Rodzik Jarosław , Bratu Iulian Alexandru 
Published: August 2022

The knowledge, perception, and behavior of young people toward ecotourism are important for the sustainable development of this sector. The purpose of this research is to evaluate and analyze the knowledge of young Romanian students about ecotourism and the main ecotourism destinations in Romania, their behavior in relation to the practice of this form of sustainable tourism at the local level in the last 2 years (2020 and 2021), and people’s use of the Internet for planning holidays and digital applications while traveling in nature. A quantitative research was conducted based on a self-administered questionnaire, structured in 13 sections on ecotourism, to which were added the sociodemographic data of the respondents. The conclusions of the research are that young people know well the main ecotourism destinations in Romania; they choose them according to the beauty of the landscape, multitude of outdoor activities, and the hospitality of the hosts. In the last 2 years of the pandemic, young people have visited several times the main existing ecotourism destinations locally, with an average vacation duration of 1–3 days. The Internet remains the main source of information, and in the field, they use mobile applications that allow the download of digital maps and recognize different plant species. By integrating various activities in nature, in close connection with the local culture, ecotourism can contribute to the sustainable use of local tourism resources. This study provides a detailed and realistic picture of the knowledge and the type of ecotourism experience that young people want. The implications are theoretical and managerial, highlighting the importance of assessing the preferences of young ecotourists, as a basis for developing ecotourism strategies, sustainable use of local tourism resources, and management of protected areas.
Urea hydrolysis in different farmland soils as affected by long-term biochar application
Authors: Zhao Rui , Liu Juying , Xu Na , He Tianyi , Meng Jun , Liu Zunqi 
Published: August 2022

Urea is a commonly used nitrogen (N) fertilizer that contributes to world food production, and there have been increasing concerns about relatively low urea-N use efficiency. Biochar has shown the potential to mitigate N loss, but how biochar influences urea hydrolysis and the underlying mechanisms are still unclear. In this study, long-term biochar-amended upland, paddy and greenhouse soils were sampled at depths of 0–20 and 20–40 cm in Haicheng City, Northeast China. Soil N contents, urea hydrolysis rates (UHRs), and total, intracellular and extracellular urease activities were determined, as well as the total bacterial and ureolytic microbial gene abundance were quantified. The results showed that biochar increased total urease activity by 32.64–66.39% in upland soil and by 2.90–2.13-fold in paddy soil. Both intracellular and extracellular ureases contributed to the increase in total urease activity. However, in greenhouse soil, extracellular (+35.07–74.22%) and intracellular (−40.14–77.68%) urease activities responded inconsistently to biochar incorporation. Increases in ureC gene copy numbers (2.15- to 4.47-fold) in upland and greenhouse (20.93%) soil implied that biochar stimulated microorganisms capable of producing urease, and the biochar liming effect increased the soil pH (0.11–0.60 units), which optimized the ureolytic reaction, together explained the increases in urease activity. We found that the decreased soil N content was accompanied by a higher UHR in upland and greenhouse soils, suggesting that the accelerated UHR exerted a negative effect on the soil N content, possibly caused by excessive NH3 volatilization. In paddy soil, where the UHR was not increased, biochar was an effective amendment for simultaneously improving soil urease activity and N content.
Genetic Predisposition to Severe COVID-19 Might Increase the Risk of Stroke: A Two-Sample Mendelian Randomization Study
Authors: Zhang Jinji , Wu Fayong , Chen Shenge , Zhu Ying , Luo Xian , Qiu Xiaolin 
Published: August 2022

Aims: The causal relationship between COVID-19 infection and stroke has not yet been fully established. This study aimed to explore this causality using two-sample Mendelian randomization (MR).Materials and Methods: Genetic variants associated with COVID-19 infection and stroke were both obtained from genome-wide association study (GWAS) summary data. The single nucleotide polymorphisms (SNPs) were selected as instrumental variables. The standard inverse variance weighted (IVW) was primarily used to assess this causality. Finally, sensitivity analysis was performed to evaluate the reliability and stability.Results: The results showed that being hospitalized due to COVID-19 had a positive effect on stroke [OR = 1.05; 95% CI= (1.01, 1.10); p = 2.34 × 10−5] and ischemic stroke [OR = 1.06; 95% CI= (1.02, 1.11); p = 2.28 × 10−6] analyzed by inverse variance weighted. Moreover, the results revealed that severe respiratory symptoms due to COVID-19 had a positive effect on stroke [OR = 1.04; 95% CI= (1.00, 1.06); p = 0.04] and that the causal effect of severe respiratory symptoms due to COVID-19 on ischemic stroke estimated by IVW suggested a positive effect [OR = 1.06; 95% CI= (1.02, 1.09); p = 0.0068], too.Conclusion: In summary, this study showed that severe COVID-19 might increase the risk of stroke, thus much more attention should be paid to patients with severe COVID-19.
APAview: A web-based platform for alternative polyadenylation analyses in hematological cancers
Authors: Hu Xi , Song Jialin , Chyr Jacqueline , Wan Jinping , Wang Xiaoyan , Du Jianqiang , Duan Junbo , Zhang Huqin , Zhou Xiaobo , Wu Xiaoming 
Published: August 2022

Background: Hematologic malignancies, such as acute promyelocytic leukemia (APL) and acute myeloid leukemia (AML), are cancers that start in blood-forming tissues and can affect the blood, bone marrow, and lymph nodes. They are often caused by genetic and molecular alterations such as mutations and gene expression changes. Alternative polyadenylation (APA) is a post-transcriptional process that regulates gene expression, and dysregulation of APA contributes to hematological malignancies. RNA-sequencing-based bioinformatic methods can identify APA sites and quantify APA usages as molecular indexes to study APA roles in disease development, diagnosis, and treatment. Unfortunately, APA data pre-processing, analysis, and visualization are time-consuming, inconsistent, and laborious. A comprehensive, user-friendly tool will greatly simplify processes for APA feature screening and mining.Results: Here, we present APAview, a web-based platform to explore APA features in hematological cancers and perform APA statistical analysis. APAview server runs on Python3 with a Flask framework and a Jinja2 templating engine. For visualization, APAview client is built on Bootstrap and Plotly. Multimodal data, such as APA quantified by QAPA/DaPars, gene expression data, and clinical information, can be uploaded to APAview and analyzed interactively. Correlation, survival, and differential analyses among user-defined groups can be performed via the web interface. Using APAview, we explored APA features in two hematological cancers, APL and AML. APAview can also be applied to other diseases by uploading different experimental data.
Community-led research for reproductive justice: Exploring the SisterLove Georgia Medication Abortion project
Authors: Mosley Elizabeth A. , Ayala Sequoia , Jah Zainab , Hailstorks Tiffany , Dixon Diallo Dázon , Hernandez Natalie , Jackson Kwajelyn , Hairston Indya , Hall Kelli S. 
Published: August 2022

IntroductionWhile reproductive injustice indicators are improving globally, they are worsening in the United States particularly for Black and other marginalized communities. Eugenics and obstetric violence against low-income and communities of color create well-founded distrust of sexual and reproductive health (SRH). Transformational, reparative ways of conducting SRH research are needed.Proposed principles of community-led research for reproductive justiceDrawing on our collective experience as reproductive justice leaders, SRH researchers, and clinicians, we propose the following principles of community-led research for reproductive justice: 1) Center the marginalized community members most affected by SRH inequities as leaders of research; 2) Facilitate equitable, collaborative partnership through all phases of SRH research; 3) Honor multiple ways of knowing (experiential, cultural, empirical) for knowledge justice and cross-directional learning across the team; 4) Build on strengths (not deficits) within the community; 5) Implement the tenets of reproductive justice including structural-level analysis and the human rights framework; 6) Prioritize disseminating useful findings to community members first then to other audiences; 7) Take action to address social and reproductive injustices.SisterLove's community-led georgia medication abortion projectWe offer the community-led Georgia Medication Abortion (GAMA) Project by reproductive justice organization SisterLove from 2018–2022 as a case study to demonstrate these principles along with the strengths and challenges of reproductive justice research.DiscussionCommunity-led reproductive justice research offers innovative and transformational methods for truly advancing SRH in an era of increasing policy restrictions and decreasing access to care. Yet existing funding, research administrative, and publishing systems will require structural change.
Ginsenoside Rg5 allosterically interacts with P2RY12 and ameliorates deep venous thrombosis by counteracting neutrophil NETosis and inflammatory response
Authors: Chen Ziyu , Wang Gaorui , Xie Xueqing , Liu Heng , Liao Jun , Shi Hailian , Chen Min , Lai Shusheng , Wang Zhengtao , Wu Xiaojun 
Published: August 2022

BackgroundDeep venous thrombosis (DVT) highly occurs in patients with severe COVID-19 and probably accounted for their high mortality. DVT formation is a time-dependent inflammatory process in which NETosis plays an important role. However, whether ginsenoside Rg5 from species of Panax genus could alleviate DVT and its underlying mechanism has not been elucidated.MethodsThe interaction between Rg5 and P2RY12 was studied by molecular docking, molecular dynamics, surface plasmon resonance (SPR), and molecular biology assays. The preventive effect of Rg5 on DVT was evaluated in inferior vena cava stasis–induced mice, and immunocytochemistry, Western blot, and calcium flux assay were performed in neutrophils from bone marrow to explore the mechanism of Rg5 in NETosis via P2RY12.ResultsRg5 allosterically interacted with P2RY12, formed stable complex, and antagonized its activity via residue E188 and R265. Rg5 ameliorated the formation of thrombus in DVT mice; accompanied by decreased release of Interleukin (IL)-6, IL-1β, and tumor necrosis factor-α in plasma; and suppressed neutrophil infiltration and neutrophil extracellular trap (NET) release. In lipopolysaccharide- and platelet-activating factor–induced neutrophils, Rg5 reduced inflammatory responses via inhibiting the activation of ERK/NF-κB signaling pathway while decreasing cellular Ca2+ concentration, thus reducing the activity and expression of peptidyl arginine deiminase 4 to prevent NETosis. The inhibitory effect on neutrophil activity was dependent on P2RY12.ConclusionsRg5 could attenuate experimental DVT by counteracting NETosis and inflammatory response in neutrophils via P2RY12, which may pave the road for its clinical application in the prevention of DVT-related disorders.
Immunogenicity and immune-persistence of the CoronaVac or Covilo inactivated COVID-19 Vaccine: a 6-month population-based cohort study
Authors: Hua Qianhui , Zhang Hangjie , Yao Pingping , Xu Nani , Sun Yisheng , Lu Hangjing , Xu Fang , Liao Yuting , Yang Juan , Mao Haiyan , Zhang Yanjun , Zhu Hanping , Hu Xiaowei , Lv Huakun , Jiang Jianmin 
Published: August 2022

BackgroundOwing to the coronavirus disease 2019 (COVID-19) pandemic and the emergency use of different types of COVID-19 vaccines, there is an urgent need to consider the effectiveness and persistence of different COVID-19 vaccines.MethodsWe investigated the immunogenicity of CoronaVac and Covilo, two inactivated vaccines against COVID-19 that each contain inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The levels of neutralizing antibodies to live SARS-CoV-2 and the inhibition rates of neutralizing antibodies to pseudovirus, as well as the immunoglobulin (Ig)G and IgM responses towards the spike (S) and nucleocapsid (N) protein of SARS-CoV-2 at 180 days after two-dose vaccination were detected.ResultsThe CoronaVac and Covilo vaccines induced similar antibody responses. Regarding neutralizing antibodies to live SARS-CoV-2, 77.9% of the CoronaVac vaccine recipients and 78.3% of the Covilo vaccine recipients (aged 18–59 years) seroconverted by 28 days after the second vaccine dose. Regarding SARS-CoV-2-specific antibodies, 97.1% of the CoronaVac vaccine recipients and 95.7% of the Covilo vaccine recipients seroconverted by 28 days after the second vaccine dose. The inhibition rates of neutralizing antibody against a pseudovirus of the SARS-CoV-2 Delta variant were significantly lower compared with those against a pseudovirus of wildtype SARS-CoV-2. Associated with participant characteristics and antibody levels, persons in the older age group and with basic disease, especially a chronic respiratory disease, tended to have lower anti-SARS-CoV-2 antibody seroconversion rates.ConclusionAntibodies that were elicited by these two inactivated COVID-19 vaccines appeared to wane following their peak after the second vaccine dose, but they persisted at detectable levels through 6 months after the second vaccine dose, and the effectiveness of these antibodies against the Delta variant of SARS-CoV-2 was lower than their effectiveness against wildtype SARS-CoV-2, which suggests that attention must be paid to the protective effectiveness, and its persistence, of COVID-19 vaccines on SARS-CoV-2 variants.
Ecological risk assessment of metallic nanoparticles on the marine environments: Species sensitivity distributions analysis
Authors: Feng Sulan , Zhu Lin , Zhao Xinguo , Sui Qi , Sun Xuemei , Chen Bijuan , Qu Keming , Xia Bin 
Published: August 2022

Metallic nanoparticles (NPs) are increasingly being used and inevitably entering the marine environment. Therefore, the adverse effects of NPs on individual marine species have attracted increasing attentions. However, to date, the ecological risks of NPs on the marine ecosystem remain poorly understood. In this study, species sensitivity distributions (SSDs) were constructed for Ag, ZnO, CuO, and TiO2 NPs to understand their ecotoxicity to the marine ecosystem and the contribution of size effect and ion effect to the nanotoxicity. Furthermore, a case study on the assessment of marine ecological risk and marine environment carrying capacity of metallic NPs was performed in Jiaozhou Bay, China. The results showed the toxicity of metallic NPs to marine species following the order of Ag &gt; ZnO &gt; CuO &gt; TiO2. Both size effect and ion effect contributed to the toxicity of NPs. Environmental concentrations of Ag, ZnO, CuO, and TiO2 NPs are much lower than the predicted no-effect concentrations, indicating that these metallic NPs have not yet posed risks to the marine ecosystem based on currently limited measured environmental concentrations of metallic NPs in seawater. And the marine environment carrying capacity of Ag, ZnO, CuO, and TiO2 NPs in Jiaozhou Bay seawater were determined to be 5.03, 8.72, 93.06, and 629.79 t, respectively. The results help us understand the ecological risk of NPs in marine environments and provide a scientific basis for the sustainable development of nanotechnology.
The top 100 most-cited articles on exercise therapy for sarcopenia: A bibliometric analysis
Authors: Guo Zhao-jing , Xie Wen-qing , Cai Zi-jun , Zhang Yue-yao , Ding Yi-lan , Naranmandakh Shinen , Li Yu-sheng , Xiao Wen-feng 
Published: August 2022

ObjectiveVast quantities of literature regarding the applications of exercise therapy for sarcopenia have been published. The main objective of this study is to determine the top 100 most-cited articles and analyze their bibliometric characteristics.DesignThis study reports a bibliometric analysis via a systematic search of the academic literature regarding the applications of exercise therapy for sarcopenia.MethodsAll databases in the Web of Science were searched with the following strategy: term search (TS) = (exercise* OR training OR “physical activit*”) AND TS = (sarcopenia) on 25 February 2022. The results were presented in descending order by their total citations. The list of the top 100 articles was finally determined by negotiation of two independent researchers.ResultsThe top 100 articles were published between 1993 and 2020. More than half of the articles (n = 54) were published during the decade 2006–2015. Total citations of the top 100 articles ranged from 155 to 1,131 with a median of 211.5. The average of annual citations was constantly increasing with year (P &lt; 0.05). The most studied exercise therapy is strength/resistance training, with about 71% articles had discussed about it. The top 100 articles were from 54 different journals, and the Journal of Applied Physiology was the journal that contributed the most articles (n = 8). A total of 75 different first corresponding authors from 15 countries made contributions to the top 100 list. Luc J.C. van Loon from the Maastricht University in the Netherlands published the most articles (n = 5) as the first corresponding author. Most articles (87%) were from North America (58%) and Europe (29%), while the United States as a country contributed over half of the articles (51%).ConclusionOur study determined the top 100 most-cited articles on exercise therapy for sarcopenia and analyzed their bibliometric characteristics, which may provide a recommended list for researchers in this field and pave the way for further research.