bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dose–response screening of industrial hemp to herbicides commonly used in corn and soybean
Authors: Ortmeier-Clarke Haleigh J. , Oliveira Maxwel C. , Arneson Nicholas J. , Conley Shawn P. , Werle Rodrigo 
Published: January 2022

AbstractRecent legalization of industrial hemp in the United States has led to increased interest among stakeholders to produce hemp for grain and fiber. However, owing to the lack of herbicides registered for use in hemp, producers are left with limited weed management strategies. Moreover, much of the agricultural land that could be used to cultivate industrial hemp may be prone to carryover of previously applied residual herbicides or physical drift from herbicides sprayed nearby. Industrial hemp sensitivity to herbicides is not well documented. Dose–response studies were conducted under controlled conditions in Madison, WI, screening two industrial hemp grain cultivars for tolerance to 44 preemergence and postemergence herbicides commonly used in corn and soybean. Treatments consisted of herbicides applied at 0×, 0.125×, 0.25×, 0.50×, 0.75×, 1×, 2×, and 4× the recommended maximum labeled rates based on soil type. Preemergence applications were delivered immediately after planting, whereas postemergence applications took place when hemp plants reached 5 to 10 cm in height. Nontreated plants served as the control and were used to estimate percent biomass reduction; dose–response curves were generated. Biomass reduction was >50% for rates under the suggested label rate for 23 preemergence and 21 postemergence herbicides tested. All herbicides tested resulted in >25% biomass reduction at the 0.125× rate, except for clopyralid applied preemergence and postemergence and saflufenacil applied preemergence. This is concerning, as the label rates are determined for effective weed control and the mitigation of herbicide resistance. Overall, these results indicate that industrial hemp is very sensitive to most herbicides tested. Growers should consider herbicide use history and surrounding crops when determining industrial hemp field selection to prevent significant plant injury due to herbicide carryover and drift. Further research into alternative methods of weed control will be vital to establishing hemp as a dominant crop once again.
Annual bluegrass weevil (Listronotus maculicollis) and paclobutrazol control annual bluegrass (Poa annua) in creeping bentgrass fairways
Authors: Diehl Katherine H. , Elmore Matthew T. , Koppenhöfer Albrecht M. , Murphy James A. , Kostromytska Olga S. 
Published: January 2022

AbstractThe annual bluegrass weevil (ABW) is a pest of fine turfgrass, but recent research has found that withholding insecticides for ABW control can reduce annual bluegrass cover. The objective of this research was to evaluate threshold-based insecticide and paclobutrazol programs for annual bluegrass control. The effect of three insecticide programs (preventive, threshold, and no insecticide) and four rates of paclobutrazol (0, 70, 105, or 210 g ha−1 applied monthly) were evaluated. Replicate experiments were conducted from April to November in both 2018 and 2019 on a mixed creeping bentgrass and annual bluegrass fairway in North Brunswick, NJ. By the conclusion of both experiments, all paclobutrazol programs exhibited reduced annual bluegrass cover compared with the nontreated plots. In threshold and no-insecticide programs, reduction in annual bluegrass cover was enhanced by paclobutrazol applied at 105 g ha−1 in both years, and at 70 g ha−1 in the 2019 experiment. Paclobutrazol at 210 g ha−1 resulted in annual bluegrass cover of <20% regardless of insecticide program. In 2019, threshold-based ABW control without paclobutrazol provided similar annual bluegrass control as monthly applications of paclobutrazol at 70 and 105 g ha−1 with the preventive insecticide program. A reduction in turfgrass quality from threshold-based insecticide programs persisted for a shorter duration than the no-insecticide program, regardless of paclobutrazol treatment. Threshold-based ABW insecticide programs that allow ABW feeding damage to occur can result in reduced annual bluegrass cover. These reductions were further enhanced by paclobutrazol applications. The combination of threshold-level insecticide with moderate rates of paclobutrazol (70 to 105 g ha−1) provided reductions in annual bluegrass cover that were similar to the highest rate of paclobutrazol (210 g ha−1) without ABW damage. Turfgrass managers who integrate the threshold-level insecticide approach and monthly paclobutrazol applications may achieve greater annual bluegrass control than either strategy alone if temporary reductions in turf quality can be tolerated.
A Post‐Phaseout Retrospective Reassessment of the Global Methyl Bromide Budget
Authors: Saltzman E. S. , Nicewonger M. R. , Montzka S. A. , Yvon‐Lewis S. A. 
Published: January 2022

AbstractMethyl bromide is a stratospheric ozone‐depleting substance with both natural and anthropogenic sources. The global budget of methyl bromide has never been fully understood as evidenced by the significant budget gap between the bottom‐up source estimates and calculated atmospheric losses. Atmospheric methyl bromide levels have declined significantly since Phase‐out under the Montreal Protocol began in 1999, and the atmosphere appears to have reached a new steady state during the past five years. Here, we reassess the global methyl bromide budget utilizing the 25‐year record of atmospheric methyl bromide measurements from the National Oceanic and Atmospheric Administration Global Monitoring Laboratory global flask network and a zonal 6‐box coupled global ocean/atmosphere model. Model inversions were used to estimate the total emissions required to account for the observed atmospheric methyl bromide levels. From 1995 to 2019, global land‐based emissions (natural and anthropogenic) declined from about 120 to 85 Gg y−1 and net ocean emissions increased from −5 to +5 Gg y−1. There remains an imbalance between the bottom‐up estimates of terrestrial sources and the inversion result. Based on the timing, magnitude, and spatial distribution of the imbalance we partition it into (a) a persistent or time invariant source located primarily in the tropics, and (b) a smaller time‐varying component that scales with the anthropogenic source during phase‐out. We hypothesize that the persistent source is likely natural and the time variant component is an artifact resulting from a slight underestimation of anthropogenic emissions.
Natural Orthogonal Component Analysis of Daily Magnetic Variations at the Martian Surface: InSight Observations
Authors: Luo H. , Du A. M. , Ge Y. S. , Johnson C. L. , Mittelholz A. , Zhang Y. , Sun S. Q. , Zhao L. , Yu Y. , Tian L. , Li S. Y. , Xu W. Y. 
Published: January 2022

AbstractDistinguishing different sources of magnetic field variations at InSight is important to understand dynamic processes in the Martian ionosphere as well as the coupling between the solar wind and the Martian induced magnetosphere. Recent studies based on magnetic field measurements from InSight have suggested that the daily and seasonal variations in the magnetic field at the Martian surface are at least partially the result of neutral wind‐driven ionospheric dynamo currents and their seasonal variations. However, the sources of the daily variations with different time scales need be further investigated. In this paper, magnetic field variations in a sol as well as during nearly a whole Martian year from InSight observations were decomposed into their natural orthogonal components. We found that the first eigenmode shows the previously identified early to midmorning peak, and varies with season. This corresponds to the solar quiet variations. The second and higher eigenmodes manifest the quasi‐Carrington and sub‐Carrington rotation periodicity represent disturbed components that may be stimulated by variations in the draped interplanetary magnetic field and/or the Martian ionospheric electron density. Different from their counterparts at the Earth, the amplitude of the first eigenmode is comparable with the sum of second to fifth ones, showing that the quiet and disturbed diurnal variations contribute similarly to the total diurnal variation. Decomposition of Martian surface magnetic field variations could provide monitoring of the Martian ionospheric current system as well as the solar wind conditions in the near‐Mars space, which will be greatly enhanced when combined with Zhurong Martian surface field measurements in the future.
Impact of limited residential address on health effect analysis of predicted air pollution in a simulation study
Authors: Jun Yoon-Bae , Song Insang , Kim Ok-Jin , Kim Sun-Young 
Published: January 2022

AbstractBackgroundRecent epidemiological studies of air pollution have adopted spatially-resolved prediction models to estimate air pollution concentrations at people’s homes. However, the benefit of these models was limited in many studies that used existing health data relying on incomplete addresses resulting from confidentiality concerns or lack of interest when designed.ObjectiveThis simulation study aimed to understand the impact of incomplete addresses on health effect estimation based on the association between particulate matter with diameter ≤10 µm (PM10) and low birth weight (LBW).MethodsWe generated true annual average concentrations of PM10at 46,007 mothers’ homes and their LBW status, using the parameters obtained from our data analysis and a previous study in Seoul, Korea. Then, we hypothesized that mothers’ address information is limited to the district and compared the properties of their health effect estimates of PM10with those using complete addresses. We performed this comparison across eight environmental scenarios that represent various spatial distributions of PM10and nine exposure prediction methods that provide different sets of predicted PM10concentrations of mothers.ResultsWe observed increased bias and root mean square error consistently across all environmental scenarios and prediction methods using incomplete addresses compared to complete addresses. However, the bias related to incomplete addresses decreased when we used population-representative exposures averaged to the district from predicted PM10at census tract centroids.SignificanceOur simulation study suggested that individual exposure estimated by prediction approaches and averaged across population-representative points can provide improved accuracy in health effect estimates when complete address data are unavailable.Impact statementOur simulation study focused on a common and practical challenge of limited address information in air pollution epidemiology, and investigated its impact on health effect analysis. Cohort studies of air pollution have developed advanced exposure prediction model to allow the estimation of individual-level long-term air pollution concentrations at people’s addresses. However, it is common that address information of existing health data is available at the coarse spatial scale such as city, district, and zip code area. Our findings can help understand the possible consequences of limited address information and provide practical guidance in achieving the accuracy in health effect analysis.
7T ultra-high-field neuroimaging for mental health: an emerging tool for precision psychiatry?
Authors: Neuner Irene , Veselinović Tanja , Ramkiran Shukti , Rajkumar Ravichandran , Schnellbaecher Gereon Johannes , Shah N. Jon 
Published: January 2022

AbstractGiven the huge symptom diversity and complexity of mental disorders, an individual approach is the most promising avenue for clinical transfer and the establishment of personalized psychiatry. However, due to technical limitations, knowledge about the neurobiological basis of mental illnesses has, to date, mainly been based on findings resulting from evaluations of average data from certain diagnostic groups. We postulate that this could change substantially through the use of the emerging ultra-high-field MRI (UHF-MRI) technology. The main advantages of UHF-MRI include high signal-to-noise ratio, resulting in higher spatial resolution and contrast and enabling individual examinations of single subjects. Thus, we used this technology to assess changes in the properties of resting-state networks over the course of therapy in a naturalistic study of two depressed patients. Significant changes in several network property measures were found in regions corresponding to prior knowledge from group-level studies. Moreover, relevant parameters were already significantly divergent in both patients at baseline. In summary, we demonstrate the feasibility of UHF-MRI for capturing individual neurobiological correlates of mental diseases. These could serve as a tool for therapy monitoring and pave the way for a truly individualized and predictive clinical approach in psychiatric care.
Immune marker levels in severe mental disorders: associations with polygenic risk scores of related mental phenotypes and psoriasis
Authors: Werner Maren Caroline Frogner , Wirgenes Katrine Verena , Shadrin Alexey , Lunding Synve Hoffart , Rødevand Linn , Hjell Gabriela , Ormerod Monica Bettina Elkjær Greenwood , Haram Marit , Agartz Ingrid , Djurovic Srdjan , Melle Ingrid , Aukrust Pål , Ueland Thor , Andreassen Ole Andreas , Steen Nils Eiel 
Published: January 2022

AbstractSeveral lines of evidence implicate immune abnormalities in the pathophysiology of severe mental disorders (SMD) and comorbid mental disorders. Here, we use the data from genome-wide association studies (GWAS) of autoimmune diseases and mental phenotypes associated with SMD to disentangle genetic susceptibilities of immune abnormalities in SMD. We included 1004 patients with SMD and 947 healthy controls (HC) and measured plasma levels of IL-1Ra, sIL-2R, gp130, sTNFR-1, IL-18, APRIL, and ICAM-1. Polygenic risk scores (PRS) of six autoimmune disorders, CRP, and 10 SMD-related mental phenotypes were calculated from GWAS. General linear models were applied to assess the association of PRS with immune marker abnormalities. We found negative associations between PRS of educational attainment and IL-1Ra (P = 0.01) and IL-18 (P = 0.01). There were nominal positive associations between PRS of psoriasis and sgp130 (P = 0.02) and PRS of anxiety and IL-18 (P = 0.03), and nominal negative associations between PRS of anxiety and sIL-2R (P = 0.02) and PRS of educational attainment and sIL-2R (P = 0.03). Associations explained minor amounts of the immune marker plasma-level difference between SMD and HC. Different PRS and immune marker associations in the SMD group compared to HC were shown for PRS of extraversion and IL-1Ra ([interaction effect (IE), P = 0.002), and nominally for PRS of openness and IL-1Ra (IE, P = 0.02) and sTNFR-1 (IE, P = 0.04). Our findings indicate polygenic susceptibilities to immune abnormalities in SMD involving genetic overlap with SMD-related mental phenotypes and psoriasis. Associations might suggest immune genetic factors of SMD subgroups characterized by autoimmune or specific mental features.
JAK2 inhibitor persistence in MPN: uncovering a central role of ERK activation
Authors: Pandey Garima , Kuykendall Andrew T. , Reuther Gary W. 
Published: January 2022

AbstractThe Philadelphia chromosome negative myeloproliferative neoplasms, including polycythemia vera, essential thrombocytosis, and myelofibrosis, are driven by hyper activation of the JAK2 tyrosine kinase, the result of mutations in three MPN driving genes: JAK2, MPL, and CALR. While the anti-inflammatory effects of JAK2 inhibitors can provide improved quality of life for many MPN patients, the upfront and persistent survival of disease-driving cells in MPN patients undergoing JAK2 inhibitor therapy thwarts potential for remission. Early studies indicated JAK2 inhibitor therapy induces heterodimeric complex formation of JAK2 with other JAK family members leading to sustained JAK2-dependent signaling. Recent work has described novel cell intrinsic details as well as cell extrinsic mechanisms that may contribute to why JAK2 inhibition may be ineffective at targeting MPN driving cells. Diverse experimental strategies aimed at uncovering mechanistic details that contribute to JAK2 inhibitor persistence have each highlighted the role of MEK/ERK activation. These approaches include, among others, phosphoproteomic analyses of JAK2 signaling as well as detailed assessment of JAK2 inhibition in mouse models of MPN. In this focused review, we highlight these and other studies that collectively suggest targeting MEK/ERK in combination with JAK2 inhibition has the potential to improve the efficacy of JAK2 inhibitors in MPN patients. As MPN patients patiently wait for improved therapies, such studies should further strengthen optimism that pre-clinical research is continuing to uncover mechanistic insights regarding the ineffectiveness of JAK2 inhibitors, which may lead to development of improved therapeutic strategies.
The EMT-induced lncRNA NR2F1-AS1 positively modulates NR2F1 expression and drives gastric cancer via miR-29a-3p/VAMP7 axis
Authors: Li Dandan , Xu Mengjie , Wang Zidi , Huang Pan , Huang Congcong , Chen Zhen , Tang Gaijuan , Zhu Xingji , Cai Mengyu , Qin Shanshan 
Published: January 2022

AbstractDeregulated lncRNAs play critical roles in tumorigenesis and tumor progression. NR2F1-AS1 is an antisense lncRNA of NR2F1. However, the biological function of NR2F1-AS1 in gastric cancer (GC) remains largely unclear. In this study, we revealed that NR2F1-AS1 and NR2F1 were both positively correlated with the degree of malignancy and predicted poor prognosis in two independent GC cohorts. Besides, NR2F1-AS1 and NR2F1 can respond to Epithelial-to-mesenchymal transition (EMT) signaling in GC, since their expression was increased by TGF-beta treatment and decreased after stable overexpression of OVOL2 in GC cell lines. NR2F1-AS1 and NR2F1 were highly co-expressed in pan-tissues and pan-cancers. Depletion of NR2F1-AS1 compromised the expression level of NR2F1 in GC cells. Furthermore, NR2F1-AS1 knockdown inhibited the proliferation, migration, invasion and G1/S transition of GC cells. More importantly, transcriptome sequencing revealed a novel ceRNA network composed of NR2F1-AS1, miR-29a-3p, and VAMP7 in GC. The overexpression of VAMP7 predicted poor prognosis in GC. Rescue assay confirmed that NR2F1-AS1 promotes GC progression through miR-29a-3p/VAMP7 axis. Our finding highlights that the aberrant expression of NR2F1-AS1 is probably due to the abnormal EMT signaling in GC. LncRNA NR2F1-AS1 plays crucial roles in GC progression by modulating miR-29a-3p/VAMP7 axis, suggesting that NR2F1-AS1 may serve as a potential therapeutic target in GC.
Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1
Authors: Qian Weiyi , Hadi Tarik , Silvestro Michele , Ma Xiao , Rivera Cristobal F. , Bajpai Apratim , Li Rui , Zhang Zijing , Qu Hengdong , Tellaoui Rayan Sleiman , Corsica Annanina , Zias Ariadne L. , Garg Karan , Maldonado Thomas , Ramkhelawon Bhama , Chen Weiqiang 
Published: January 2022

AbstractMechanical overload of the vascular wall is a pathological hallmark of life-threatening abdominal aortic aneurysms (AAA). However, how this mechanical stress resonates at the unicellular level of vascular smooth muscle cells (VSMC) is undefined. Here we show defective mechano-phenotype signatures of VSMC in AAA measured with ultrasound tweezers-based micromechanical system and single-cell RNA sequencing technique. Theoretical modelling predicts that cytoskeleton alterations fuel cell membrane tension of VSMC, thereby modulating their mechanoallostatic responses which are validated by live micromechanical measurements. Mechanistically, VSMC gradually adopt a mechanically solid-like state by upregulating cytoskeleton crosslinker, α-actinin2, in the presence of AAA-promoting signal, Netrin-1, thereby directly powering the activity of mechanosensory ion channel Piezo1. Inhibition of Piezo1 prevents mice from developing AAA by alleviating pathological vascular remodeling. Our findings demonstrate that deviations of mechanosensation behaviors of VSMC is detrimental for AAA and identifies Piezo1 as a novel culprit of mechanically fatigued aorta in AAA.
Engineering the stambomycin modular polyketide synthase yields 37-membered mini-stambomycins
Authors: Su Li , Hôtel Laurence , Paris Cédric , Chepkirui Clara , Brachmann Alexander O. , Piel Jörn , Jacob Christophe , Aigle Bertrand , Weissman Kira J. 
Published: January 2022

AbstractThe modular organization of the type I polyketide synthases (PKSs) would seem propitious for rational engineering of desirable analogous. However, despite decades of efforts, such experiments remain largely inefficient. Here, we combine multiple, state-of-the-art approaches to reprogram the stambomycin PKS by deleting seven internal modules. One system produces the target 37-membered mini-stambomycin metabolites − a reduction in chain length of 14 carbons relative to the 51-membered parental compounds − but also substantial quantities of shunt metabolites. Our data also support an unprecedented off-loading mechanism of such stalled intermediates involving the C-terminal thioesterase domain of the PKS. The mini-stambomycin yields are reduced relative to wild type, likely reflecting the poor tolerance of the modules downstream of the modified interfaces to the non-native substrates. Overall, we identify factors contributing to the productivity of engineered whole assembly lines, but our findings also highlight the need for further research to increase production titers.
On-surface synthesis and characterization of nitrogen-substituted undecacenes
Authors: Eimre Kristjan , Urgel José I. , Hayashi Hironobu , Di Giovannantonio Marco , Ruffieux Pascal , Sato Shizuka , Otomo Satoru , Chan Yee Seng , Aratani Naoki , Passerone Daniele , Gröning Oliver , Yamada Hiroko , Fasel Roman , Pignedoli Carlo A. 
Published: January 2022

AbstractHeteroatom substitution in acenes allows tailoring of their remarkable electronic properties, expected to include spin-polarization and magnetism for larger members of the acene family. Here, we present a strategy for the on-surface synthesis of three undecacene analogs substituted with four nitrogen atoms on an Au(111) substrate, by employing specifically designed diethano-bridged precursors. A similarly designed precursor is used to synthesize the pristine undecacene molecule. By comparing experimental features of scanning probe microscopy with ab initio simulations, we demonstrate that the ground state of the synthesized tetraazaundecacene has considerable open-shell character on Au(111). Additionally, we demonstrate that the electronegative nitrogen atoms induce a considerable shift in energy level alignment compared to the pristine undecacene, and that the introduction of hydro-aza groups causes local anti-aromaticity in the synthesized compounds. Our work provides access to the precise fabrication of nitrogen-substituted acenes and their analogs, potential building-blocks of organic electronics and spintronics, and a rich playground to explore π-electron correlation.