bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Incubation Near an Inverted Pyramidal Structure on Adsorption Properties of an Enzyme
Authors: Ivanov Yuri D. , Tatur Vadim Yu. , Pleshakova Tatyana O. , Shumov Ivan D. , Kozlov Andrey F. , Valueva Anastasia A. , Ivanova Irina A. , Ershova Maria O. , Ivanova Nina D. , Stepanov Igor N. , Lukyanitsa Andrei A. , Konev Vladimir A. , Ziborov Vadim S. 
Published: March 2022

Abstract
The incubation of a solution of horseradish peroxidase (HRP) enzyme near an inverted pyramid (IP) has been found to influence the enzyme's aggregation and adsorption properties. Atomic force microscopy (AFM) has been employed to study the adsorption of HRP onto mica substrates at the single-molecule level, while spectrophotometry has been used as a reference method for the estimation of its enzymatic activity. By AFM, we have revealed an increase in the relative content of HRP oligomers adsorbed onto mica substrates after the incubation of the HRP solutions below the IP's apex in comparison with the control HRP solution. The same situation has been observed after the incubation of the enzyme solution above the center of the IP's base. At that, the enzymatic activity of HRP remained virtually unchanged in the both cases. Since pyramidal structures of positive and inverted orientation are employed in biosensor devices, the results obtained in our study are important to be taken into account in the development of highly sensitive biosensor systems.
Asiaticoside expedites recovery of diabetic ulcers through activation of Wnt1/β-catenin signaling cascade
Authors: Li Jun , Zhang Hao , Tan Qian 
Published: March 2022

Abstract
The prevalence of diabetic ulcers (DU) are on the rise, which are the most common diabetic complications, and characterized as a type of chronic and refractory wound. The specific mechanisms of DU formation remain unclarified, and the therapeutic methods also are unsatisfactory. In this study, we found asiaticoside (AC) facilitated the diabetic rats’ skin wound recovery. Intensive studies demonstrated that AC boosted fibroblast proliferation and migration in the high-glyceic environment. Mechanically, AC up-regulated Wnt1 in protein and immunohistochemistry level, and activate Wnt/β-Catenin signaling cascade, thereby promoting fibroblast functions. Thus, AC boosts skin cell activities relevant to DU may have therapeutic value.
CYBA C242T polymorphism is a risk factor for pre-eclampsia:a case control study
Authors: Zhang Ru , Liu Shuhui , Tan Ping , Hou Huabin , Guo Zihan , Liu Shiguo , Li Hui , Wang Xibing 
Published: March 2022

Abstract
Background: Although the mechanisms responsible for pathogenesis of preeclampsia(PE) have not been entirely clarified, oxidative stress are thought to be its leading cause. As a major component responsible for ROS production during oxidative stresses, p22phox, encoded by CYBA, is one essential subunit of Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. The aim of this study is to investigate whether CYBA expression and its polymorphism were associated with PE. Methods: The expression of CYBA was analyzed in placenta of PE and control group, as well as in the HTR-8 / Svneo cells stimulated with CoCL2 and TNF-α, respectively. Then, the CYBA C242T polymorphism in 1184 patients with PE and 1421 healthy controls was genotyped by TaqMan probe, and the different distributions were confirmed by a case-control association study. Results: The expression of CYBA mRNA and protein in placenta of pregnant women with PE increased significantly. The expression of CYBA mRNA was also increased in HTR-8/Svneo cells, collected after 24 hours separately stimulated by cobalt chloride and TNF-α. In addition, there was no significant difference in the distribution of C242T locus genotype and allele frequency of CYBA between the case group and the control group (P>0.05). Conclusions: CYBA may play a role in the pathogenesis of oxidative stress in PE, in which it may function via cooperating with TNF-a related inflammatory pathway. Although there was no discrepant distribution of CYBA C242T polymorphism in Chinese population, it is necessary to perform the genetic studies on more CYBA SNPs for multiple regions to gain further insights into its pathogenesis.
Development of Molecular Markers and Identification of Germplasm Resources Based on SLAF-Seq Technology in Lilii Bulbus (Bai-He in Chinese)
Authors: Yuan Yu-Han , Peng Jin-Hai , Yang Ao-Ao , Xia Ao-Nan , Wang Yan , Wang Liang , Liu Yun-Guo 
Published: March 2022

Abstract
In order to analyze the genetic evolution of 29 Lilii Bulbus (Baihe), high-density single nucleotide polymorphism (SNPs) marks, and a specific length amplified fragment sequences (SLAFs) were constructed. As a result of sequencing, 76.92 Mb reads were detected. The data of reads for each sample ranged from 1,356,347 to 5,736,545. The average of Q30 and GC content were 93.26% and 36.09%, respectively. In addition, 247,458 high-quality SLAF tags were obtained with the average sequencing depth of 11.07x, among which 135,981 polymorphic SLAF tags and 4,238,446 SNP tags were developed. Phylogenetic tree analysis of different sequences showed that 29 Lilii Bulbus germplasm resources clustered in the same clade. Our sequencing data of SLAF-seq profiling clues to potential genes identification and genetic diversity analysis study. The present useful genetic information of Lilii Bulbus, and the SNP molecular markers will provide a theoretical basis for genetic association studies of Lilii Bulbus germplasm resources.
Are patients on chronic hemodialysis treatment protected against mortality due to COVID-19? A single-center retrospective cohort of patients hospitalized with COVID-19 requiring hemodialysis treatment
Authors: Juha Márk , Kóczy Ágnes , Ledó Nóra , Tislér András , Takács István , Tabák Ádám , Kevei Péter , Pethő Ákos Géza 
Published: March 2022

Abstract
Background: It is widely accepted that SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), the pathogen causing COVID-19 (coronavirus disease 2019), not only affects the respiratory system but can damage other organs, including the kidneys. We aimed to assess the mortality of hospitalized COVID-19 patients who received hemodialysis treatment (patients with previously normal renal function - nCKD, patients with chronic kidney disease previously not requiring hemodialysis - CKDnonHD, and CKD patients on regular hemodialysis - pHD). Material and methods: All COVID-19 patients admitted to our institution (01/OCT/2020-31/MAY/2021) who received hemodialysis treatment were followed up for all-cause mortality in the central database of the National Health Service until 01/DEC/2021. Data on demography, presenting signs and symptoms, and laboratory results at baseline were drawn from hospital charts. All-cause mortality was compared between nCKD, CKDnonHD, and pHD groups in crude and adjusted Cox-proportional hazard models.Results: 83 of 108 (76.9%) were included in the analysis due to missing covariates. Over a median 26 (interquartile range 11-266) days of follow-up 20 of 22 (90.9%) of nCKD, 23 of 24 (95.8%) of CKDnonHD, and 17 of 37 (45.9%) of pHD patients died. All groups were similar in terms of age and sex distribution. In general, nCKD patients had fewer comorbidities (hypertension, diabetes mellitus, ischemic heart disease) but more severe presentations (dyspnea, O2 saturation, C-reactive protein, white blood cell count, and albumin). In contrast, pHD patients had the least severe presentation. In a model adjusted for independent predictors of all-cause mortality (C-reactive protein and serum albumin), CKDnonHD patients had increased (hazard ratio [HR] 1.91, 95% confidence interval [CI] 1.02-3.60), while pHD patients decreased mortality (HR 0.41, 95% CI 0.20-0.81) compared to nCKD patients. After further adjustment for the need for intensive care treatment, the difference in mortality between the nCKD and the pHD groups became non-significant.Conclusion: Patients requiring acute hemodialysis during hospitalization due to COVID-19 had a remarkably high all-cause mortality. While CKD patients who were previously not requiring hemodialysis fared worse than those without known CKD before hospitalization, the better survival of chronic hemodialysis patients suggests the role of unmeasured confounding or selection bias related to the hospitalization of even asymptomatic patients to prevent the spread of disease during transport to treatment.
“To Do, or Not to Do?”: Determinants of Stakeholders’ Acceptance on Dengue Vaccine Using PLS-SEM Analysis between Scientists and Public in Malaysia.
Authors: Arham Ahmad Firdhaus , Amin Latifah , Mustapa Muhammad Adzran Che , Mahadi Zurina , Yaacob Mashitoh , Arham Ahmad Fadhly , Norizan Nor Sabrena 
Published: March 2022

Abstract
Background: Dengue vaccine is the best potential alternative for protecting communities and cities from dengue. Nevertheless, the dengue vaccine’s acceptance must be considered a guide for authorities to continue intensified research and, eventually, execute dengue vaccine injections to improve public health. Therefore, this study aimed to determine the relationship between the predictor factors that influence stakeholders’ attitude and intention towards the dengue vaccine.Methods: Survey data collected from 399 respondents which represent two primary stakeholder groups: scientist (n = 202) and public (n = 197) were analysed by producing a Partial Least Square-Structural Equation Modelling (PLS-SEM) model using Smart Partial Least Square (Smart-PLS) software. Results: The findings indicated a strong positive relationship between attitudes and intentions to support the dengue vaccine, followed by a perceived benefit. The perceived benefit was the most significant predictor for attitude to dengue vaccine, followed by religiosity, attitudes to technology, and trust in key players. The findings provide insights into the multi-dimensional association of the determinants of attitude-intention to support the dengue vaccine. Conclusion: Although the findings showed that stakeholders in Malaysia are optimistic about the dengue vaccine, the effectiveness of this vaccine has not been tested in Malaysia. Hence, the ongoing research must be intensified. Therefore, this study can be used to guide decision-making on the execution of the dengue vaccine not only in Malaysia but also in other countries with a history of serious dengue transmission.
Investigate of Iran Recurrence Earthquakes by the Network
Authors: Rezaei Soghra , Taghizadeh-Farahmand Fataneh 
Published: March 2022

Abstract
Human beings have encountered numerous natural disasters over time, that the earthquake phenomenon is one of the most damaging of all and has a very complex nature. Earthquakes manifest spatio-temporal complex behavior that can be studied using complex networks, which enabled us to recognize the global features of Earthquake. Studies on the earthquakes using the network method are based on how the network is constructed. In this paper, by constructing the network, the spatio-temporal recurrence of earthquakes occurring in the Zagros region, which is recorded at all the broadband and short-period stations of the Iranian Seismological Center (ISC, http://irsc.ut.ac.ir), are subject to statistical review. The results showed that in the Zagros area the spatio recurrence is about 0.020 km, and the time recurrence for all the thresholds of magnitude is similar to power relationship (1⁄Tα) with a α =-0.9 .
POC1A, a Novel Prognostic Biomarker that Correlated With Immunosuppressive Microenvironment in Pan-cancer
Authors: Zhao Qi , Gao Shuping , Chen Xin , Zhu Xiyan 
Published: March 2022

Abstract
The effect of POC1 centriolar protein A (POC1A) in pan-cancer remains uncertain. In this study, POC1A expression in tumor tissues and normal tissues was analyzed using data from the Cancer Genome Atlas (TCGA) database and the Genotype-Tissue Expression (GTEx) project. Prognostic value and clinicopathological features of POC1A were assessed using the TCGA cohort. The correlation between POC1A and immune cell infiltration of TCGA samples download from ImmuCellAI and TIMER2 databases was explored. The association between POC1A and tumor mutation burden (TMB), microsatellite instability (MSI), and immune checkpoints genes was also assessed. In addition, gene set enrichment analysis (GSEA) was used to explore the potential molecular mechanism of POC1A in pan-cancer. We found that POC1A was highly expressed in almost all 33 tumors. High expression of POC1A was significantly associated with poor prognosis in most cases. Further, POC1A expression correlated with tumor immune infiltration and tumor microenvironment. In addition, POC1A expression was associated with immune checkpoint genes and TMB and MSI in pan-cancer. GSEA analysis revealed that POC1A is involved in the cell cycle-related and immune-related pathways.These results may elucidate the crucial roles played by POC1A in pan-cancer and identify POC1A as a potential novel prognosis biomarker and immunotherapy target in pan-cancer.
Physical and chemical characteristics of suspended sediment in plain river network
Authors: Tian Xueqi , Wang Hua , Shen Zilin , Yuan Weihao , Zeng Yichuan , Li Yuanyuan 
Published: March 2022

Abstract
The suspended movement of sediment in the plain river network is a major part of the river's sand transport, and the associated movement of suspended sand particles also influences the associated movement of pollutants in the water column. This paper selects plain river network and lakeside river network as the research area: In this paper, a plain river network and a lakeside river network are selected as the study area: the flow velocity zones are divided; the particle characteristics (geometric mean particle size GD, roundness RO, roughness FD) of the different flow velocity zones are compared; the influence of the flow velocity characteristics of the river network on the physical characteristics and enrichment capacity of suspended sand particles is assessed; the differences in elemental species and contents between different flow velocity waters are compared; the influence of the physical and chemical characteristics on the enrichment capacity of particulate heavy metals Ni is assessed. The results show that (1) The highest grain GD (5.77), roundness (1.32) and roughness (1.080) were found in the strong flow area of the Liangxi River. There was little difference in particle roughness between flow zones. (2) The average percentage content of the characteristic elements in the different flow velocity regions is generally consistent from highest to lowest, with Mg being the characteristic element with the highest average percentage content in each region. (3) The GD, FD, RO of suspended sand particles are independent of TN, TP, V, SSC and are positively correlated with flow velocity, with the particle size larger and more spherical in shape at high flow velocities in the lakeside network. (4) The enrichment factor AE = aF1(GD, RO, FD) was used to better characterize the sorption capacity of suspended sand particles for Ni than the GD factor alone, with the highest Ni sorption by suspended sand particles in the slow-flowing plain river network (EF = 3.65; AE = 4.74).
High Yield Solution Growth of FACsPbBr3 Single Crystals with Low Defect Density for Gamma-ray Spectroscopy
Authors: Huang Jinsong , Zhao Liang , Zhou Ying , Shi Zhifang , Ni Zhenyi , Liu Ye 
Published: March 2022

Abstract
Metal halide perovskites are promising candidates for room temperature gamma-ray spectrum detectors, however, it is challenging to grow high-quality single crystals under operation conditions to make them economically competitive to existing materials. Here, we report the growth of centimeter-size formamidinium-cesium lead bromide (FACsPbBr3) single crystals at high yield from low purity (98%) and thus low-cost precursors using a relatively low-temperature solution method. The introduction of FA into CsPbBr3 reduces defect density in crystals by eliminating the phase transition during the cooling from the growth temperature to room temperature, which significantly improves the yield of crack-free and low trap density crystals. As-grown FACsPbBr3 exhibits a high resistivity of 9.5×109 Ω cm, balanced hole and electron mobility-lifetime products of (2.2–3.2) ×10− 3 cm2 V− 1, a long charge recombination lifetime of 182 ns, and a record low deep trap density of 5.6 × 1010 cm− 3, which eventually results in a high charge collection efficiency of 84% under gamma-ray. The FACsPbBr3 spectrum detectors with an asymmetrical metal electrode configuration have achieved an energy resolution of 2.9% for 662 keV 137Cs γ-rays. The improved spectrum capability allows them to resolve Pb Kα,β X-rays (75–85 keV) for the first time in 137Cs spectrum. FACsPbBr3 single crystals show excellent stability under large biases up to 1000 volts, and no degradation of spectrum performance after 7 months.
What dress code do we teach students and residents?: A survey of patients' and their families' preferences regarding physicians' appearance
Authors: Goto Michiko , Sakamoto Ryota , Wakabayashi Hideki , Takemura Yousuke 
Published: March 2022

Abstract
Background: From the late 1960s, physician's appearance has been actively studied in the West, and until the early twenty-first century, patients tended to prefer “formal dress” attire along with white coats for physicians. However, people’s attitudes today toward dress code has not been clarified while it may have changed.Objective: This study aimed to investigate the acceptability of dress codes from the patient’s perspective to suggest the future of dress codes.Methods: Outpatients and their families in a university hospital and small-to medium-sized hospital were surveyed. We inquired which of the different styles of white coats and different colors of scrubs were most desirable for male and female physicians. We used Scheffet’s pair-comparison method to show the rankings.Results: Patients and their families expected their physicians to wear white coats rather than scrubs. Furthermore, the more traditional and formal dress code was preferred. The least preferred color of scrubs was yellow.Conclusion: The study found that patient’s preference for a traditional, conservative appearance has not changed over time. It did not match today's perspective on infection prevention. Although, both perspectives, patient preferences and infection prevention are important for education and gaining patient trust.
Optimizing Kinetics of Anionic Redox Reaction in Cathode Material by Distinct 4d Transition-metal Substitution
Authors: Dai Kehua , Zhao Beibei , Shao Weiwei , Zhang Wenjuan , mao jing , Wu Jinpeng , Zhuo Zengqing , Zhang Liang , Li Ning , Tong Wei , Liu Gao , Yang Wanli 
Published: March 2022

Abstract
Anionic redox reactions (ARR) offer significant opportunities for developing high-energy-density batteries. However, suffering from the sluggish anionic kinetics, the concept meets serious challenges on practical application. Recently, the interplay between cationic and anionic redox processes on kinetics is revealed and the mixed cationic/anionic redox process shows faster kinetics. In this work, we further find that stronger TM(4d)–O(2p) (e. g. Ru–O) hybridization improves the kinetics of independent ARR. The redox reactions of Na2/3Mg1/3Mn1/2Ru1/6O2 (NMMR) and their evolution of Ru, Mn, and O during charging and discharging is studied in detail. The O and TM redox process in initial cycle are distinguished. Then, the different influence on kinetics in ARR and TM redox stages by Ru substitution is unraveled. The significantly improved Na+ diffusion coefficient in NMMR indicates stronger TM(4d)–O(2p) (Ru–O) hybridization does optimize the kinetics of ARR.
Drug Treatment Courts and Community-Level Crime: A Stacked Event Study Analysis
Authors: Hibbard Patrick 
Published: March 2022

Abstract
Objectives: Explore the extent to which adult drug treatment courts (ADCs) affect community-level crime, demonstrate the application of stacked event study estimation in quasi-experimental criminology research, and introduce a crime data optimization strategy. Methods: Static and dynamic fixed effects estimation were performed on a longitudinal dataset constructed to address potential issues of bias, which included crime data linked to a unique list of ADCs and relevant control variables, resulting in over 52,000 county-year observations between 1990 and 2018. Results: Crime reduction estimates range from 2% property index to a violent index drop of 6%. Counterfactual analysis indicates over 417,000 fewer crimes within this sample between 1995 and 2013 (which accounts for the stack design). Conclusions: Considering their limited footprint, ADCs have an outsized effect on crime alluding to complex interactions between participants and communities. Though further investigation is needed, expanding these programs would likely continue to improve public safety.
Women’s experience and perspectives toward genital cosmetic surgery: A qualitative study
Authors: Safari Kolsoom , Piro Tiran Jamil , Saeed Awaz Aziz , Hassan Warda 
Published: March 2022

Abstract
Background Nowadays female genital cosmetic surgery (FGCS) has become quite popular, which has led to a broad variety of medical and social implications. After these surgeries, most women experienced anxiety, depression, and other psychiatric conditions. The present study was carried out in order to explore women’s perspective and experience toward genital cosmetic surgery based on their context and cultural aspects.Methods This study is a qualitative content analysis and collected data were analyzed through the approach introduced by Graneheim and Lundman (2004) after interview with nine women who experienced genital cosmetic surgeries in the city of Erbil, Kurdistan-Iraq in 2021-2022. ResultsAfter semi-structured interviews which carried out with participants, the main themes, including “husband satisfaction” and “self-confidence” have been extracted. Some relevant subthemes and meaning units like “ugly appearance”, “anxiety related to husband undesirable intercourse”, and “dyspareunia” were retrieved from the participants’ stories, and the related conclusions of each theme were explored.Conclusion Study findings suggest that female genital cosmetic surgery improves the women’s body image and sexual function and the couples’ sexual satisfaction, especially that of husbands. Because of people sociocultural aspects in Kurdistan region, they need to be more aware of female sexual needs and marital relationship. To investigate the issue more, conduction of further studies is recommended.