bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robotic-assisted closed-chest management of a fungal-infected prosthetic aortic graft: a case report
Authors: Giammarino Ashley T , Sarmiento Iam Claire , Scheinerman SJacob , Winalski John , Lazzaro Richard S , Brinster Derek R , Hemli Jonathan M 
Published: May 2022

Abstract
Background
Fungal prosthetic graft infections are associated with high mortality, typically requiring aggressive surgical debridement. We present an alternative, minimally invasive approach to address these challenging clinical cases.

Case presentation
A 76-year-old Caucasian male with prior aortic root and arch replacement presented with localized chest wall tenderness after being hit by a car door. Computed tomography angiogram incidentally identified fluid in the anterior mediastinum, surrounding his ascending aortic graft. Rather than undertaking a high-risk reoperative sternotomy and redo complex aortic reconstruction, we elected to proceed with a robotic-assisted, minimally invasive debridement of the aortic graft, coupled with an omental wrap, entirely within the closed chest. Microbiology was positive for Aspergillus species. The patient made an uncomplicated recovery and was discharged home on antifungal therapy, likely to continue indefinitely.

Conclusions
Infected prosthetic aortic grafts can be successfully managed with debridement and pedicled omental flap coverage via a minimally invasive approach within the closed chest, obviating the morbidity of a complex reoperative open procedure.
Pseudomonas species prevalence, protein analysis, and antibiotic resistance: an evolving public health challenge
Authors: Elbehiry Ayman , Marzouk Eman , Aldubaib Musaad , Moussa Ihab , Abalkhail Adil , Ibrahem Mai , Hamada Mohamed , Sindi Wael , Alzaben Feras , Almuzaini Abdulaziz Mohammad , Algammal Abdelazeem M. , Rawway Mohammed 
Published: May 2022

AbstractPsychrotrophic Pseudomonas is one of the significant microbes that lead to putrefaction in chilled meat. One of the biggest problems in the detection of Pseudomonas is that several species are seemingly identical. Currently, antibiotic resistance is one of the most significant challenges facing the world's health and food security. Therefore, this study was designed to apply an accurate technique for eliminating the identification discrepancy of Pseudomonas species and to study their resistance against various antimicrobials. A total of 320 chicken meat specimens were cultivated, and the isolated bacteria’ were phenotypically recognized. Protein analysis was carried out for cultured isolates via Microflex LT. The resistance of Pseudomonas isolates was recorded through Vitek® 2 AST-GN83 cards. Overall, 69 samples were identified as Pseudomonas spp. and included 18 Pseudomonas lundensis (P. lundensis), 16 Pseudomonas fragi (P. fragi), 13 Pseudomonas oryzihabitans (P. oryzihabitans), 10 Pseudomonas stutzeri (P. stutzeri), 5 Pseudomonas fluorescens (P. fluorescens), 4 Pseudomonas putida (P. putida), and 3 Pseudomonas aeruginosa (P. aeruginosa) isolates. Microflex LT identified all Pseudomonas isolates (100%) correctly with a score value ≥ 2.00. PCA positively discriminated the identified isolates into various groups. The antimicrobial resistance levels against Pseudomonas isolates were 81.16% for nitrofurantoin, 71% for ampicillin and ampicillin/sulbactam, 65.22% for cefuroxime and ceftriaxone, 55% for aztreonam, and 49.28% for ciprofloxacin. The susceptibilities were 100% for cefotaxime, 98.55% for ceftazidime, 94.20% for each piperacillin/tazobactam and cefepime, 91.3% for cefazolin. In conclusion, chicken meat was found to be contaminated with different Pseudomonas spp., with high incidence rates of P. lundensis. Microflex LT is a potent tool for distinguishing Pseudomonads at the species level.
A new paradigm for diagnosis of neurodegenerative diseases: peripheral exosomes of brain origin
Authors: Younas Neelam , Fernandez Flores Leticia Camila , Hopfner Franziska , Höglinger Günter U. , Zerr Inga 
Published: May 2022

AbstractNeurodegenerative diseases are a heterogeneous group of maladies, characterized by progressive loss of neurons. These diseases involve an intricate pattern of cross-talk between different types of cells to maintain specific signaling pathways. A component of such intercellular cross-talk is the exchange of various types of extracellular vesicles (EVs). Exosomes are a subset of EVs, which are increasingly being known for the role they play in the pathogenesis and progression of neurodegenerative diseases, e.g., synucleinopathies and tauopathies. The ability of the central nervous system exosomes to cross the blood–brain barrier into blood has generated enthusiasm in their study as potential biomarkers. However, the lack of standardized, efficient, and ultra-sensitive methods for the isolation and detection of brain-derived exosomes has hampered the development of effective biomarkers. Exosomes mirror heterogeneous biological changes that occur during the progression of these incurable illnesses, potentially offering a more comprehensive outlook of neurodegenerative disease diagnosis, progression and treatment. In this review, we aim to discuss the challenges and opportunities of peripheral biofluid-based brain-exosomes in the diagnosis and biomarker discovery of Alzheimer’s and Parkinson’s diseases. In the later part, we discuss the traditional and emerging methods used for the isolation of exosomes and compare their advantages and disadvantages in clinical settings.
Suitability of Hermetia illucens larvae meal and fat in broiler diets: effects on animal performance, apparent ileal digestibility, gut histology, and microbial metabolites
Authors: Hartinger Kristina , Fröschl Katharina , Ebbing Marco Antonio , Bruschek-Pfleger Barbara , Schedle Karl , Schwarz Christiane , Gierus Martin 
Published: May 2022

Abstract
Background
The possibility of partially replacing soybean meal (SBM) with Hermetia illucens (HI) defatted larvae meal in broiler nutrition has frequently been suggested. For sustainability reasons, however, the larvae fat produced during defatting should also be used and could be particularly beneficial regarding gut health due to its fatty acid composition. To evaluate the suitability of HI larvae as protein and fat source, a 2 × 3 factorial arrangement with two types of protein, i.e. SBM (S) or SBM and 15% of its crude protein replaced by HI larvae meal (L), and three levels of fat sources, namely 0 (0 L), 50% (50 L) or 100% HI larvae fat (100 L) at the expense of soybean oil was applied.

Results
In the starter phase, an interaction showed higher body weight (BW), average daily gain (ADG) and improved feed conversion ratio (FCR) if 50% or 100% HI larvae fat was fed with HI larvae meal. Moreover, BW, ADG and FCR improved when feeding HI larvae meal as protein source. Additionally, we observed an increased average daily feed intake in the grower, finisher, and overall phase in the L groups and an improved FCR in 0 L compared to 50 L groups during the overall period. Regarding apparent ileal digestibility, HI larvae meal feeding increased dry matter, organic matter, and fat digestibility. Feeding HI larvae meal as protein source decreased the concentrations of agmatine, spermidine, spermine and ammonia in the caecal digesta, whereas fat source affected agmatine with higher concentrations in 50 L compared to 0 L in the colonic digesta. In contrast, caecal ethanolamine concentrations increased in HI larvae meal groups compared to SBM. Caecal butyric acid concentrations decreased with HI larvae meal feeding. An interaction was found for the jejunal villus area, being higher in L + 100 L compared to S + 100 L. Furthermore, L groups had greater villus width.

Conclusions
A partial replacement of SBM with HI larvae meal and soybean oil with HI larvae fat in broiler diets without impairing animal performance or gut health seems possible. Feeding HI larvae meal affected broiler performance positively in the starter phase and improved apparent ileal digestibility.
Psychometric properties of the Farsi version of the Eating Pathology Symptoms Inventory (F-EPSI) among Iranian University men and women
Authors: Sahlan Reza N. , Blomquist Kerstin K. , Bodell Lindsay P. 
Published: May 2022

Abstract
Background
Limited research has validated eating pathology assessments in Iranian men and women. The purpose of the current study was to translate and validate a Farsi version of the Eating Pathology Symptoms Inventory (F-EPSI) in Iranian university students.

Methods
Men (n = 279) and women (n = 486) completed questionnaires including the F-EPSI.

Results
Confirmatory factor analysis (CFA) indicated that the F-EPSI had an acceptable fit to the data and supported the eight-factor model. The scale was partially invariant across genders. Men reported higher scores on Excessive Exercise and Muscle Building subscales, and women reported higher scores on Body Dissatisfaction and Restricting subscales. The F-EPSI subscales had good 5- to 6-month test–retest reliability. The F-EPSI demonstrated convergent validity with clinical impairment, eating pathology, and body mass index (BMI). Finally, individuals scoring above the Clinical Impairment Assessment (CIA) cutoffs reported higher scores on the F-EPSI subscales, further supporting convergent validity of the scale.

Conclusion
Findings suggest that the F-EPSI will enable researchers to examine eating pathology symptoms among men and women in Iran.
Horizontal augmentation techniques in the mandible: a systematic review
Authors: Smeets Ralf , Matthies Levi , Windisch Peter , Gosau Martin , Jung Ronald , Brodala Nadine , Stefanini Martina , Kleinheinz Johannes , Payer Michael , Henningsen Anders , Al-Nawas Bilal , Knipfer Christian 
Published: May 2022

AbstractPurposePlacement of dental implants has evolved to be an advantageous treatment option for rehabilitation of the fully or partially edentulous mandible. In case of extensive horizontal bone resorption, the bone volume needs to be augmented prior to or during implant placement in order to obtain dental rehabilitation and maximize implant survival and success.MethodsOur aim was to systematically review the available data on lateral augmentation techniques in the horizontally compromised mandible considering all grafting protocols using xenogeneic, synthetic, or allogeneic material. A computerized and manual literature search was performed for clinical studies (published January 1995 to March 2021).ResultsEight studies ultimately met the inclusion criteria comprising a total of 276 procedures of xenogeneic, allogeneic, or autogenous bone graft applications in horizontal ridge defects. Particulate materials as well as bone blocks were used as grafts with a mean follow-up of 26.0 months across all included studies. Outcome measures, approaches and materials varied from study to study. A gain of horizontal bone width of the mandible with a mean of 4.8 mm was observed in seven of eight studies. All but one study, reported low bone graft failure rates of 4.4% in average.ConclusionsOnly limited data are available on the impact of different horizontal augmentation strategies in the mandible. The results show outcomes for xenogeneic as well as autologous bone materials for horizontal ridge augmentation of the lower jaw. The use of allogeneic bone-block grafts in combination with resorbable barrier membranes must be re-evaluated. Randomized controlled clinical trials are largely missing.
Additive and epistatic effects influence spectral tuning in molluscan retinochrome opsin
Authors: Smedley G. Dalton , McElroy Kyle E. , Feller Kathryn D. , Serb Jeanne M. 
Published: May 2022

ABSTRACT
The relationship between genotype and phenotype is non-trivial because of the often complex molecular pathways that make it difficult to unambiguously relate phenotypes to specific genotypes. Photopigments, comprising an opsin apoprotein bound to a light-absorbing chromophore, present an opportunity to directly relate the amino acid sequence to an absorbance peak phenotype (λmax). We examined this relationship by conducting a series of site-directed mutagenesis experiments of retinochrome, a non-visual opsin, from two closely related species: the common bay scallop, Argopecten irradians, and the king scallop, Pecten maximus. Using protein folding models, we identified three amino acid sites of likely functional importance and expressed mutated retinochrome proteins in vitro. Our results show that the mutation of amino acids lining the opsin binding pocket is responsible for fine spectral tuning, or small changes in the λmax of these light-sensitive proteins. Mutations resulted in a blue or red shift as predicted, but with dissimilar magnitudes. Shifts ranged from a 16 nm blue shift to a 12 nm red shift from the wild-type λmax. These mutations do not show an additive effect, but rather suggest the presence of epistatic interactions. This work highlights the importance of binding pocket shape in the evolution of spectral tuning and builds on our ability to relate genotypic changes to phenotypes in an emerging model for opsin functional analysis.
Ιδεολογήματα και εννοιολογήσεις στην οργάνωση και λειτουργία των Πρότυπων Πειραματικών Σχολείων
Authors: ΚΑΛΕΡΑΝΤΕ ΕΥΑΓΓΕΛΙΑ 
Published: May 2022

ρυθμίσεις(2011/2015) για τα Πρότυπα Πειραματικά Σχολεία αλλά και την πολιτική των προθέσεων, με βάση τις οικονομικές, πολιτικές και κοινωνικές συνθήκες, που ως«αφηγήσεις» ενεργοποιούνται, ως εσωτερικοί αυτοματισμοί και θέτουν ζητήματα για τη λειτουργικότητα των θεσμών, όπως της εκπαίδευσης.Διερευνούμε τη νομοθετική εξέλιξη (2011/2015) ενός θεσμού και ιδιαίτερα των ρυθμίσεων και προϋποθέσεων εισαγωγής των μαθητών στα ιδιαίτερου τύπου σχολεία. Ειδικότερα, με βάση τα κριτήρια επιλογής, εννοιολογούνται θέματα για την αξιολόγηση κάποιου ιδιαίτερου προσόντος ή δεξιότητας. Παράλληλα, συνεξετάζουμε, τις περιόδους που υπήρχε αξιολόγηση για την εισαγωγή των μαθητώνσε αυτόν τον τύπο σχολείων, το περιεχόμενό της και συνακόλουθα το μαθητικό κοινό στο οποίο απευθυνόταν συγκριτικά με το νέο διαμορφούμενο νομοθετικό πλαίσιο της επιλογής με κλήρωση.Ταυτόχρονα, ανιχνεύουμε κοινωνικές νοηματοδοτήσεις και συμβολικές υποστασιοποιήσεις για τις έννοιες, χαρισματικό άτομο, άτομο με υψηλή νοημοσύνη, που διαμορφώνουν ένα πλαίσιο, σημασιακών τάξεων αναφοράς στην αξία της«διαφοράς», για τη δημιουργία ιδιαίτερων σχολείων. Στόχος μας είναι να συσχετίσουμε την εξελικτική λειτουργία των ιδιαίτερων σχολείων για χαρισματικά άτομα με την ταξική κατανομή και τις φιλελεύθερες διευθετήσεις, που ενισχύουν-με την επίκληση του «δικαιώματος στη διαφορά»- περιεχόμενο θέσεων, αξιοποιώντας πολιτικές δομές του έθνους-κράτους αλλά και παγκοσμιοποιημένες πολιτικές αποφάσεις. Τα νέα ιδεολογήματα που προτάσσονται, ως ipso iure, μέσα από τα νέα φετιχ- τον «εκσυγχρονισμό», την «καινοτομία», την «αναδόμηση»- επιβάλλουν και νομιμοποιούν, ως «κατασκευή», την «εκπαιδευτική ορθότητα» διευρυμένου τύπου διαφοροποιήσεων. Ίσως ο τελευταίος νόμος του 2015 αναιρεί τις εκπαιδευτικές δομικές διαφοροποιήσεις, επαναφέροντας το διάλογο στην ανάγκη αναμόρφωσης της δημόσιας παιδείας με διευρυμένες δομές ευκαιρίας.Λέξεις-κλειδιά: πειραματικά-πρότυπα σχολεία, χαρισματικά άτομα, νομοθεσία, διαφορετικότητα, δημόσια παιδεία, δομές ευκαιρίας
ASEAN and the Frankenstein dilemma: Will ASEAN become a monster?
Authors: Syam M Husni , An Aqimuddin Eka 
Published: May 2022

Background: International organizations are pivotal as actors in international relations. Although its establishment represents a state, they have their objectives separate from their creators. The Association of Southeast Asian Nations [ASEAN] was created to handle cooperation and maintain peace and security in Southeast Asia. Although ASEAN was equipped with institutional organs to fulfil its purposes, member states' influence is unavoidable. This situation raises the Frankenstein dilemma in which creators cannot fully control its creature. This research aims to elaborate on ASEAN as a separate institution with independent objectives and how far member states can manage ASEAN as its creation. Methods: This study uses descriptive-analytical legal research as a method to examine ASEAN constitutional instruments and its first legal text to elaborate on its capacity as a legal person and its role in distinguishing interests between member states. The two ASEAN constitutional instruments are the Bangkok Declaration, 1967, and The ASEAN Charter, 2007, while the first legal instrument is the Treaty of Amity and Cooperation. All of the resources were gathered from ASEAN legal instruments database. Results: After carefully examining the documents, since its establishment ASEAN has kept member states at arms-length to realize their interests; the ASEAN position is only to legalize what its member state wants. Even after 40 years, this situation has not changed. While ASEAN has independent objectives and structures, its member states always made institutional designs to control ASEAN's capacity to act in the international forum. In this case, ASEAN is always under the authority of its member states and has never evolved like Frankenstein's creature. Conclusions: ASEAN will never turn into a monster as ASEAN never has sole interests that contradict its members. ASEAN member states always design ASEAN constitutional and legal instruments to restrict its role as an independent legal person in international forums.
Drainage culverts as a measure to avoid mammal roadkills in Costa Rica: the case of Dasyprocta punctata
Authors: Monge-Velázquez Michelle , Sáenz Joel C. 
Published: May 2022

Los pasos de fauna son estructuras que conectan hábitats, poblaciones y reducen la mortalidad de la vida silvestre. En sitios donde no hay mitigación vial, la fauna puede recurrir a estructuras de drenaje subterráneas llamadas alcantarillas, para evitar el cruce directo por la carretera. Para determinar de manera general qué especies de mamíferos se ven beneficiadas por estas estructuras, colocamos cámaras trampa en 6 alcantarillas y comparamos cuantificando las especies de mamíferos que mueren atropelladas mediante recorridos en vehículo a lo largo de 30 km de la carretera Interamericana Norte en Costa Rica. Detectamos 14 especies de mamíferos utilizando las alcantarillas como pasos de fauna, siendo el agutí centroamericano (Dasyprocta punctata) la especie con mayor número de registros. La ausencia de registros de atropello de D. punctata y la alta tasa de uso de alcantarillas sugiere que estas estructuras pueden ser efectivas en la mitigación de atropellamiento para esta especie y son un medio para cruzar de un bloque boscoso a otro.
Abortion policy implementation in Ireland: Lessons from the community model of care
Authors: Mishtal Joanna , Reeves Karli , Chakravarty Dyuti , Grimes Lorraine , Stifani Bianca , Chavkin Wendy , Duffy Deirdre , Favier Mary , Horgan Patricia , Murphy Mark , Lavelanet Antonella F. 
Published: May 2022

Background
In 2018, the right to lawful abortion in the Republic of Ireland significantly expanded, and service provision commenced on 1 January, 2019. Community provision of early medical abortion to 9 weeks plus 6 days gestation delivered by General Practitioners constitutes the backbone of the Irish abortion policy implementation. We conducted a study in 2020–2021 to examine the barriers and facilitators of the Irish abortion policy implementation.

Methods
We collected data using qualitative in-depth interviews (IDIs) which were conducted in-person or remotely. We coded and analysed interview transcripts following the grounded theory approach.

Results
We collected 108 IDIs in Ireland from May 2020 to March 2021. This article draws on 79 IDIs with three participant samples directly relevant to the community model of care: (a) 27 key informants involved in the abortion policy development and implementation representing government healthcare administration, medical professionals, and advocacy organisations, (b) 22 healthcare providers involved in abortion provision in community settings, and (c) 30 service users who sought abortion services in 2020. Facilitators of community-based abortion provision have been: a collaborative approach between the Irish government and the medical community to develop the model of care, and strong support systems for providers. The MyOptions helpline for service users is a successful national referral model. The main barriers to provision are the mandatory 3-day wait, unclear or slow referral pathways from primary to hospital care, barriers for migrants, and a shortage and incomplete geographic distribution of providers, especially in rural areas.

Conclusions
We conclude that access to abortion care in Ireland has been greatly expanded since the policy implementation in 2019. The community delivery of care and the national helpline constitute key features of the Irish abortion policy implementation that could be duplicated in other contexts and countries. Several challenges to full abortion policy implementation remain.
Registration quality and availability of publications for clinical trials in Germany and the influence of structural factors
Authors: Thiele Christian , Hirschfeld Gerrit 
Published: May 2022

Introduction
Analyses of clinical trial registries (CTRs) offer insights into methodological problems of published research studies, e.g., non-publication and outcome-switching. Here, we use CTRs as a tool to evaluate clinical studies conducted in Germany and test how their registration quality is associated with time and structural factors: Coordinating Centers for Clinical Trials (KKS) and Universities of Excellence.

Methods
We searched ClinicalTrials.gov, the DRKS, and the ICTRP for clinical trials recruiting participants in Germany. As a measure for the methodological quality, we assessed the proportion of trials that were pre-registered. In addition, the registration quality and availability of publications relating to the trials were manually assessed for a sample (n = 639). Also, the influence of the structural factors was tested using regression models.

Results
We identified 35,912 trials that were conducted in Germany. 59% of trials were pre-registered. Surprisingly, Universities of Excellence had lower pre-registration rates. The influence of KKS was unclear and also difficult to test. Interventional trials were more likely to be pre-registered. Registration quality improved over time and was higher in interventional trials. As of early 2021, 49% of trials that started until the end of 2015 have published scientific articles. 187 of 502 studies on ClinicalTrials.gov for which we found published articles did not reference any in the registry entry.

Discussion
The structural predictors did not show consistent relationships with the various outcome variables. However, the finding that the study type and time were related to better registration quality suggests that regulatory regimes may have an impact. Limitations of this non-pre-registered study were that no modifications to registry entries were tracked and the coarse measure of KKS involvement.