bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of a versatile aptasensing chip for aflatoxin B1 in photothermal and electrochemical dual modes
Authors: Wang Chengquan , Zhao Xin , Gu Chengdong , Xu Foyan , Zhang Wuhao , Huang Xingyi , Qian Jing 
Published: April 2022

Abstract
The dual-mode sensing strategy not only has the inherent characteristics of each response mode, but also can mutually verify the detection results obtained by different modes, which will effectively improve the accuracy and reliability of detection. The electrochemical-photothermal dual-mode not only has two mode signals, but also has the advantages of fast response, high sensitivity, good selectivity, low cost and simple operation. Efficient integration and accurate detection of pathogenic bacteria to achieve early warning of food safety is one of the most valuable antifouling methods. Here, we report an integrated design strategy to establish a photothermal and electrochemical dual modes for sensitive detection of aflatoxin B1 (AFB1). The aptasensor was fabricated by loading the Au@Fe3O4 onto the indium tin oxides (ITO) conductive glass modified AuNPs nano-layer (ITO/AuNPs) surface through DNA hybridization between aptamer and cDNA. After adding AFB1, aptamer tends to form aptamer-AFB1 complex, resulting in part of Au@Fe3O4 falling off the ITO/AuNPs surface and entering the reaction solution. 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2 system produces color change under the catalysis of Au@Fe3O4 and realizes the conversion of light and heat under the excitation of near-infrared light. Photothermal signal analysis is realized with the help of thermometer. In addition, combined with electrochemical impedance spectroscopy analysis with ITO/AuNPs electrode, a dual channel method is provided for AFB1 detection. Under the optimal conditions, the developed aptasensor realized sensitive and specific detection of AFB1 from 10 pg mL− 1 to 300 ng mL− 1 with low detection limit of 5 pg mL− 1 in photothermal mode. Importantly, this work provided a promising prospect for the application of photothermal effect coupled with electrochemical aptasensor in food safety.
Sleep Duration and Risk of Atherosclerosis --A Mendelian Randomization Study
Authors: Han Xu , Xiaozhuo Xu , Huang Yilin 
Published: April 2022

Abstract
Background:Observational studies have suggested associations between sleep duration and atherosclerosis(AS).However,it’s role as a risk factor or as a subeffect because of atherosclerosis is uncertain.Methods:We performed a literature search of genome-wide studies that evaluate sleep duration.We used a two -sample Mendelian randomization approach to evaluate the causality of sleep duration on atherosclerosis risk,using 2 cohorts:MRC-IEU (n=460,099)and UK Biobank(n=361,194).The two-sample MR study was conducted to estimate causal relationship between sleep duration and AS,with the inverse-variance weighted (IVW)method,Robust adjusted profile score (RAPS),simple-and weighted-median method.Moreover,MR-Egger regression,RadialMR,MR-PRESSO and leave-one-out analysis were performed to evaluate the potential pleiotropy effect.Results:Genome-wide association studies of sleep duration were evaluated.No associations were found in relation to atherosclerosis(odds ratio=0.90[95% CI,0.98-1.00];p-value=1.86×10−1).MR-Egger,MR-PRESSO,and leave-one-out analysis did not indicate horizontal pleiotropy.Conclusion:Our MR study suggested that genetically predicted sleep duration was not causally associated with AS among the European populations.
Robotic Colorectal Surgery - Assessment of the Quality of Patient Information Available on the Internet using WebScraping
Authors: Taha-Mehlitz Stephanie , Ochs Vincent , Taha Anas 
Published: April 2022

Abstract
Background
The primary goal of this study is to assess the current patient information available on the internet concerning robotic colorectal surgery. The study is a review, and acquiring this information is relevant to understanding the subject's current and future scope.
Methods
We acquired data for this study through a web-scraping algorithm. The algorithm used two Python packages: Beautiful Soup and Selenium. The long-chain keywords incorporated into the Google, Bing and Yahoo search engines for this study were "Da Vinci Colon-Rectal Surgery", “Colorectal Robotic Surgery” and “Robotic Bowel Surgery”. After sorting the 207 websites, 5 investigators evaluated the websites according to the Ensuring quality information for patients (EQIP) score.
Results
Of the 207 websites visited, 49 belonged to the subgroup of hospital websites (23.6%), 46 to Medical Centers (22.2%), 45 to Practitioners (21.7%), 42 to Health Care Systems (20,2%), 11 to News Services (5.3%), 7 to Web Portals (3.3%), 5 to the Industry (2.4%), and 2 to Patient Groups (0.9%).
Conclusion
The quality of the information available on the internet concerning robotic colorectal surgery is low since only 82 websites received a high rating. Most of the information on the different websites is inaccurate, consequently medical facilities involved in robotic colorectal surgery, robotic bowel surgery and related should develop credible websites to guide patient decisions.
Early-stage antibody kinetics after the third dose of BNT162b2 mRNA COVID-19 vaccination measured by a point-of-care fingertip whole blood testing
Authors: Hagiya Hideharu , Nakano Yasuhiro , Furukawa Masanori , Sunada Naruhiko , Hasegawa Toru , Sakurada Yasue , Hasegawa Kou , Yamamoto Koichiro , Ogawa Hiroko , Obara Takafumi , Ageta Kouhei , Matsumoto Naomi , Matsuo Rumi , Kadowaki Tomoka , Higashikage Akihito , Hikita Takao , Yorifuji Takashi , Toyooka Shinichi , Maeda Yoshinobu , Yokokura Yoshinori , Otsuka Fumio , Nakayama Masanori 
Published: April 2022

Abstract
Background
Amid the Coronavirus Disease 2019 pandemic, we aimed to demonstrate the accuracy of the fingertip whole blood sampling test (FWT) in measuring the antibody titer and uncovering its dynamics shortly after booster vaccination.
Methods
Mokobio SARS-CoV-2 IgM & IgG Quantum Dot immunoassay (Mokobio Biotechnology R&D Center Inc., MD, USA) was used as a point-of-care FWT in 226 health care workers (HCWs) who had received two doses of the BNT162b2 mRNA vaccine (Pfizer-BioNTech) at least 8 months prior. Each participant tested their antibody titers before and after the third-dose booster up to 14-days.
Results
The effect of the booster was observed as early as the fourth day after vaccination, which exceeded the detection limit (> 30,000 U/mL) by 2.3% on the fifth day, 12.2% on the sixth day, and 22.5% after the seventh day. Significant positive correlations were observed between the pre- and post-vaccination (the seventh and eighth days) antibody titers (correlation coefficient, 0.405; p < 0.001).
Conclusion
FWT is useful for examining antibody titers as a point-of-care test. Rapid response of antibody titer started as early as the fourth day post-vaccination, while the presence of weak responders to mRNA vaccination was indicated.
A survey of retracted articles in the three high-impact journals
Authors: Tang Xiaowei , Zou Kang , Huang Shu , Jiang Jiao , Xu Huan , Pu Xinxin , Lü Yinqin , Peng Yan , Lü Muhan 
Published: April 2022

Abstract
Objectives: The effect of a retracted publication can be significant, especially in high-impact journals. We aimed to identify the characteristics of retracted articles in three high-impact clinical journals, including The Journal of the American Medical Association (JAMA), Lancet, and The New England Journal of Medicine (NEJM).Methods: The Retraction Watch database was searched from inception to March 21, 2021. Data collected for each article included title, article type, reasons for retraction, journal, publisher, publication year, retraction year, first author, country of origin, and times a retracted article was cited. Data analysis was performed by GraphPad Prism version 8.00 (La Jolla, CA, United States), and SPSS Statistics 26.0. Results: A total of 77 articles were retracted, of which 20 were from JAMA, 31 from Lancet, and 26 from NEJM. Over one-half of papers were retracted within the last decade (n=44, 57.1%). Since 2005, the number of retracted articles had increased dramatically. The United States (n=29, 37.7%) was the country with the highest number of retracted publications in the three journals, followed by the United Kingdom (n=9, 11.7%) and China (n=5, 6.5%). The most common reasons for retractions were “Error” (n=20, 26.0%), “Fraud” (n=19, 24.7%) and “Reliability” (n=18, 23.4%) in these three journals. The media number of citations was 79 (range 0-2677). The media time to retraction was 651 (range 2-8474) days. The time was longer when an article was retracted because of fraud than that because of error. Conclusions: By analyzing the features of retracted articles in the three high-impact journals, we could identify the reason for retraction more comprehensively and benefit in improving the quality of biomedical literature.
A Comparison of Spindle Activity Measurements: Automated Spindle Detection Helps Distinguish Changes in Spindle Activity
Authors: Palepu Kalyan , Sadeghi Kolia , Kleinschmidt Dave , Donoghue Jacob , Chapman Seth , Arslan Alex , Westover M. Brandon , Cash Sydney S. , Pathmanathan Jay 
Published: April 2022

Abstract
Study Objectives.
Sleep spindle activity is commonly estimated by measuring sigma power during stage 2 non-rapid eye movement (NREM2) sleep. However, spindles account for little of the total NREM2 interval and therefore sigma power over the entire interval may be misleading. This study compares derived spindle measures from direct automated spindle detection with those from gross power spectral analyses for the purposes of clinical trial design.
Methods.
We estimated spindle activity in a set of 8,440 overnight electroencephalogram (EEG) recordings from 5,793 patients from the Sleep Heart Health Study using both sigma power and direct automated spindle detection. Performance of the two methods was evaluated by ability to distinguish NREM2 from other NREM sleep stages and by determining the sample size required to detect decline in age-related spindle coherence with each method in a simulated clinical trial.
Results.
Mean sigma power in NREM2 was indistinguishable from other NREM states in 25% of recordings, vs only 7% for direct spindle measurement (P < 0.001). These percentages were 34% and 14% respectively for sigma coherence comparisons. In a simulated clinical trial, sigma power required a sample size of 115 to achieve 95% power to identify age-related changes in sigma coherence, while automated spindle detection required a sample size of only 60.
Conclusions.
Measurements of spindle activity utilizing automated spindle detection outperformed conventional sigma power analysis by a wide margin, suggesting that many studies would benefit from incorporation of automated spindle detection. These results further suggest that some previous studies which have failed to detect changes in sigma power or coherence may have failed simply because they were underpowered.
Disruptive lysosomal-metabolic signaling and neurodevelopmental deficits that precede Purkinje cell loss in a mouse model of Niemann-Pick Type-C disease
Authors: Kim Sarah , Ochoa Kathleen , Melli Sierra , Yousufzai Fawad , Zerian Barrera , Williams Aela , McIntyre Gianna , Delgado Esteban , Bolish James , Wilson Vincent , Maloney Kelly , Padron Zachary , Khan Amaal , Blanco Rosa , Soto Ileana 
Published: April 2022

Abstract
Purkinje cell (PC) loss occurs at an early age in patients and animal models of Niemann-Pick Type C (NPC), a lysosomal storage disease caused by mutations in the Npc1 or Npc2 genes. Degeneration of PCs occurs early in NPC, however little is known about how NPC1 deficiency affects the postnatal development of PCs. Using the Npc1nmf164 mouse model, we found that NPC1 deficiency significantly affected the postnatal development of PC dendrites and synapses. The developing dendrites of Npc1nmf164 PCs were significantly deficient in mitochondria and lysosomes. Furthermore, anabolic (mTORC1) and catabolic (TFEB) signaling pathways were not only perturbed but simultaneously activated in NPC1-deficient PCs, suggesting a loss of metabolic balance. We also found that mice with conditional heterozygous deletion of the Phosphatase and Tensin Homolog Deleted on Chromosome 10 gene (Pten-cHet), an inhibitor of mTORC1, showed similar early dendritic alterations in PCs to those found in Npc1-deficient mice. However, in contrast to Npc1nmf164 mice, Pten-cHet mice exhibited the overactivation of the mTORC1 pathway but with a strong inhibition of TFEB signaling, along with no dendritic mitochondrial reductions by the end of their postnatal development. Our data suggest that disruption of the lysosomal-metabolic signaling in PCs causes dendritic and synaptic developmental deficits that precede and promote their early degeneration in NPC.
CEP55 3’-UTR promotes epithelial–mesenchymal transition and enhances tumorigenicity of bladder cancer cells by acting as a ceRNA regulating miR- 497-5p
Authors: Yang Chenglin , Yang Yue , Wang Wei , Zhou Wuer , Zhang Xiaoming , Xiao Yuansong , Zhang Huifen 
Published: April 2022

Abstract
Background: Centrosomal protein 55 (CEP55) is implicated in the tumorigenesis of bladder cancer (BC) but the detailed molecular mechanisms are unknown. We aim to develop a potential competing endogenous RNA (ceRNA) network related with CEP55 in BC.Methods: We first extracted the expression profiles of RNAs from The Cancer Genome Atlas (TCGA) database and used bioinformatic analysis to establish ceRNAs in BC. Real-time quantity PCR (RT-qPCR) and immunohistochemical analysis were performed to measure CEP55 expression in different bladder cell lines and different grades of cancer. Bioinformatics analysis and luciferase assays were conducted to predict potential binding sites among miR-497-5p, CEP55, parathyroid hormone like hormone (PTHLH) and high mobility group A2 (HMGA2). Tumor xenograft model was used to show the effect of CEP55 3’-UTR on cisplatin therapy. Bioinformatics analysis, luciferase assays, and 5' rapid amplification of cDNA ends (5’RACE) were to explore the function of CEP55 3’-untranslated region (3’-UTR) on targeting miR-497-5p. Western blot and immunofluorescence assays were to detect the epithelial–mesenchymal transition (EMT) induction of CEP55 3’-UTR.Results: CEP55 expression as well as the expression levels of the oncogenic proteins PTHLH and HMGA2 were upregulated in BC cells while miR-497-5p was downregulated. Low miR-497-5p expression and high CEP55 and HMGA2 expression levels were associated with more advanced tumor clinical stage and pathological grade. Overexpression of the CEP55 3’-UTR promoted the proliferation, migration, and invasion of the EJ cell line in vitro and accelerated EJ-derived tumor growth in nude mice, while inhibition of the CEP55 3’-UTR suppressed all of these oncogenic processes. In addition, CEP55 3’-UTR upregulation reduced the cisplatin sensitivity of BC cell lines and xenograft tumors. Bioinformatics analysis, luciferase assays, and 5’RACE suggested that the CEP55 3’-UTR functions as a ceRNA targeting miR-497-5p, leading to miR-497-5p downregulation and disinhibition of PTHLH and HMGA2 expression. Further, CEP55 downregulated miR-497-5p transcription by promoting NF-kB signaling. In turn, PTHLH and HMGA2 activated p38MAPK and ERK 1/2 pathways, and induced EMT of BC cells. Conclusions: These results suggest that a ceRNA regulatory network involving CEP55 upregulates PTHLH and HMGA2 expression by suppressing endogenous miR-497-5p in bladder cancer.
Does covering prepared sterile back tables with Innovative Cover better than recommended Two-drape method?
Authors: Zarei Mohammadreza , Babajani-Vafsi Saeed , Kazemi-Galougahi Mohammad Hassan , Bakhshi Ashraf , Ajorpaz Neda Mirbagher , Ghorbani Mahdi 
Published: April 2022

Abstract
Background: Surgical site infection (SSIs) are the most significant postoperative complication. We hypothesized that (1) covering the PSBTs in an operating room (OR) during static and dynamic testing would significantly decrease the bioburden of airborne bacteria-carrying particles (ABCPs) settling on surface of them, (2) using Innovative Cover (IC) would be more effective in decreasing the bioburden of ABCPs than Two-drape method during static and dynamic testing and (3) starting airborne bacterial contamination (ABC) of the uncovered PSBTs during static testing would have longer than the same PSBTs during dynamic testing. Methods: The bioburden of ABCPs of the PSBTs was evaluated by 6 agar settle plates (n= 918 in total) on each PSBT in static and dynamic OR conditions. At each time-point, this set-up was repeated on 2 occasions else during data collection, establishing 81 PSBTs in total. Tested groups included PSBTs covered with Two-drape method, IC, or no cover. Plates were collected after 15, 30, 45, 60, 120, 180, 240 min and 24 h.Results: Covering the PSBT during static and dynamic OR conditions lead to a signiﬁcantly decreased bioburden of ABCPs on the PSBT (P < 0.05). No differences were seen between recommended Two-drape method by Association of Perioperative Registered Nurses (AORN) and IC (P > 0.05).Conclusions: Our results showed a signiﬁcant decrease in bioburden of ABCPs on the PSBTs when the they were covered during static and dynamic OR conditions, indicating the efficiency for covering the PSBTs during periods of nonuse and during active surgery. We found that there is no preference for using IC than Two-drape method.
The molecular prognostic score , a classifier for risk stratification of high-grade serous ovarian cancer
Authors: Sarkar Siddik , Saha Sarbar Ali , Sarkar Poulomi , Banerjee Sarthak , Mitra Pralay 
Published: April 2022

Abstract
Background
Clinopathological parameters such as age, residual tumor, grade and stage are often used to predict the survival of ovarian cancer patients, but still the 5-year survival of high grade serous ovarian cancer remains > 30%. Here we established a molecular gene signature-based scoring system using data of ovarian cancer cohorts that could potentially determine the median overall survival of high-grade serous ovarian cancer patients.
Methods
The data mining and analysis of raw expression data spanning over HGSOC cohorts (n = 4784) deposited in various data repositories were performed. The feature extraction/ selection tool using Cox, LASSO regression was conducted on training data to obtain predicted genes along with the coefficients that contribute to obtaining molecular prognostic score (mPS). The receiver operator characteristics curve were plotted to study prediction efficiency of mPS .
findings:
The 20 gene-based mPS predicted the 5-year overall survival with 70% efficiency both in training (n = 491) and test datasets (n = 491) and also applicable in training OTTA-SPOT HGSOC samples (n = 3762). The mPS has significant impact (HR [95%CI] = 6.1 [3.65–10.3]; p < 0.0001) on prognosis of HGSOC and prediction is more sensitive and specific as compared to clinopathological parameters: FIGO, age, residual disease. It was found that focal-adheson, Wnt, Notch signaling pathways are significantly upregulated whereas antigen processing and presentation are downregulated in high risk HGSOC cohorts.
Interpretation:
The molecular prognostic score derived from 20-gene signature is the prognostic marker or the risk classifier of HGSOC. It could be potentially harnessed in clinical settings to determine the overall survival of ovarian cancer.
Imperatorin inhibits proliferation, migration and inflammation via blocking NF-κB and MAPK pathways in rheumatoid fibroblast-like synoviocytes
Authors: Liu Mei , Lin Wei , Chen Gang , Mao Yuhang , Ma Xuemei , Zhou Junnan , Yu Xiaolu , Wang Chaoliang 
Published: April 2022

Abstract
Background: Rheumatoid arthritis (RA) is a chronic joint inflammatory disease that is associated with the aberrant activation of fibroblast‐like synoviocytes (FLSs). Searching for natural compounds that may suppress the activation of FLSs has become a complementary approach for RA treatment. Here, we investigated the effects and mechanisms of imperatorin (IPT) on proliferation, migration, and inflammation in primary cultured arthritic FLSs.Methods: Cell viability was evaluated by an MTS assay. The effects of IPT on FLS proliferation, apoptosis and migration were determined by the EdU incorporation assay, flow cytometry and wound healing assay, respectively. Inflammation was assessed by detecting the expression of multiple pro-inflammatory cytokines using quantitative real-time PCR. Western blot and immunofluorescent staining were used to analyze the potential underlying molecular mechanisms. Statistical comparisons were performed using one-way ANOVA, followed by Tukey’s post hoc analysis.Results: we found that IPT significantly suppressed TNFα-induced proliferation and migration of arthritic FLSs, but showed little effects on survival and apoptosis. In addition, IPT treatment significantly reduced TNFα-induced transcripts of proinflammatory cytokines (IL-1β, TNFα, IL-6 and IL-8) in arthritic FLSs. Further mechanism studies suggested that IPT inhibited the activations of p38 and ERK. Also, IPT blocked NF-κB activation via suppressing degradation of IκBα, and preventing the translocation of p65.Conclusion: Our results demonstrated that IPT could inhibit the over-activated phenotypes of arthritic FLSs via MAPK (p38 and ERK) and NF-KB pathways. These findings suggested IPT has the potential to be developed as a novel agent for RA treatment.
Xanthoma of Rib: A Case Report and Review of the Literature
Authors: Mottola Emilia , Adotti Flavia , Pernazza Angelina , Rocca Carlo Della , D’Amati Giulia , Nardis Piergiorgio , Vannucci Jacopo , Bassi Massimiliano , Venuta Federico , Anile Marco 
Published: April 2022

Abstract
Background: Xanthomas are well-circumscribed benign proliferative lesions seen mainly in soft tissues. Usually, they are found in hyperlipidemia and hyperlipoproteinemia family. Histologically, are characterized by macrophage-like mononuclear cells, multinucleated giant cells and abundant foam cells. The bone involvement, however, is notoriously rare and rib localization is extremely rare. Case presentation: A 55-year-old man performed a chest X-Ray and a subsequent chest Computed Tomography (CT) scan showing a rib lesion that was surgically removed and a diagnosis of rib xanthoma was made. The patient presented an unknown condition of hyperlipidemia. Conclusion: Rib xanthoma can be discovered accidentally and can be helpful in identifying an unrecognized condition of hyperlipidemia.