bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative study of robotic and laparoscopic radical resection for locally advanced gastric cancer on the dissection of lymph nodes in the upper pancreas and the effect on the pancreas
Authors: xuqi shen , Dazhi Xie , Junhui Jiang , Di Zhang , Haiyan Bao , Hao Yan , Hui An , Hongbin Tian , Wang Wang Zhonghua , Jian Miao , Yongliang Zhao 
Published: June 2022

Abstract
Purpose By comparing the short-term clinical outcome of robotic gastrectomy versus laparoscopic gastrectomy in D2 radical gastrectomy for locally advanced gastric cancer, we explored whether robotic surgery has advantages in suprapancreatic lymph node dissection and examined its effect on the pancreas. Methods We collected the clinical data of 210 consecutive patients with locally advanced gastric cancer who had undergone radical gastrectomy at our center between September 2017 and June 2018. Two groups of patients were analysed: the robotic group (n = 95) and the laparoscopic group (n = 115). Operative outcomes and postoperative complications as well as pathological data were statistically analysed. Results In cases of total radical gastrectomy, the robotic group was associated with lower intraoperative blood loss compared with the laparoscopic group (108.17±92.33 VS 142.00±80.23) mL (P=0.045). Among overweight patients with BMI>25, the robotic group still showed lower intraoperative blood loss in comparison with the laparoscopic group (126.60±80.80 VS 149.11±93.23) mL (P=0.041). In cases of distal subtotal radical gastrectomy, the number of harvested No.9 lymph nodes, No.11P lymph nodes, station 2 lymph nodes and suprapancreatic lymph nodes was higher in the robotic group as compared with the laparoscopic group (p =0.043,0.034, 0.01,0.01). In cases of total radical gastrectomy, the robotic group revealed more harvested NO.8a lymph nodes, station 2 lymph nodes and suprapancreatic lymph nodes than that in the laparoscopic group（P =0.026，0.043,0.03）. Whether or not the patients were overweight, the robotic group was associated with more harvested lymph nodes（P=0.001，0.036）.The robotic group was associated with lower drainage fluid amylase levels on postoperative day 1 and lower blood amylase on postoperative day 2 and day 3 when compared with the laparoscopic group （P=0.043，0.014，0.001）. The robotic group reported 5 cases（5.3%）of biochemical leak compared with 16 cases（13.9%） in the laparoscopic group（P=0.038). Among patients with BMI＜25(kg/m2), the robotic group showed lower levels of blood amylase on postoperative day 3. Among patients with BMI≥25(kg/m2), the robotic group showed lower ascitic fluid amylase level on postoperative day 1, lower blood amylase level on postoperative day 2 and less intraabdominal drainage liquid（P=0.043，0.008，0.043）on postoperative day 1.Conclusion In cases of D2 radical gastrectomy for locally advanced gastric cancer, the robotic group was associated with less blood loss and more harvested perigastric lymph nodes in the suprapancreatic area as well as less pancreatic injury compared with the laparoscopic group. Among overweight patients, the robotic group showed advantages as well.
A natural pyrazolotriazine pseudoiodinine produced by Pseudomonas mosselii 923 inhibits bacterial and fungal rice pathogens
Authors: Chen Gongyou , Yang Ruihuan , Shi Qing , Huang Tingting , Yan Yichao , Li Shengzhang , Fang Yuan , Li Ying , Liu Linlin , Liu Longyu , Wang Xiaozheng , Peng Yongzheng , Zou Lifang , Lin Shuangjun 
Published: June 2022

Abstract
Natural products (NPs) are a consistent source of antimicrobial metabolites and pesticide leads and are largely produced by Pseudomonads-like soil-dwelling microorganisms. Herein we report the isolation of Pseudomonas mosselii strain 923 from rice rhizosphere soils of paddy fields; this strain specifically inhibited the growth of rice bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc) and the fungal pathogen Magnaporthe oryzae. The antimicrobial compound produced by P. mosselii 923 was purified and identified as pseudoiodinine using HRMS, NMR and single-crystal X-ray diffraction. Genome-wide random mutagenesis, transcriptome analysis and biochemical assays were used to define the pseudoiodinine biosynthetic cluster as a seven-gene operon, which was designated psdABCDEFG. Pseudoiodinine biosynthesis is proposed to initiate from GTP, which is converted into 2,5-diamino-6-(5-phospho-d-ribosylamino) pyrimidin-4(3H)-one by PsdD and converted to 5-amino-6-(5-phospho-d-ribitylamino) uracil by PsdG, followed by cyclization with glycine by PsdC/PsdE and an unknown ring contraction and two methylation reactions by PsdA and PsdF. Transposon mutagenesis indicated that pseudoiodinine biosynthesis is mediated by the global regulator GacA. Further regulation is mediated by two noncoding small RNAs, rsmY and rsmZ, that positively regulate pseudoiodinine transcription, and the carbon storage regulators CsrA2 and CsrA3, which negatively regulate expression. A 22.4-fold increase in pseudoiodinine production was achieved by optimizing the media used for fermentation, overexpressing the biosynthetic operon, and removing the CsrA binding sites. Use of P. mosselii strain 923 and purified pseudoiodinine in planta revealed that both of them inhibited X. oryzae without affecting the rice host, suggesting that pseudoiodinine can be used to control plant diseases.
Intimate partner violence linked to gambling: Cohort and period effects on the past experiences of older women
Authors: Hing Nerilee , O'Mullan Catherine , Mainey Lydia , Nuske Elaine , Breen Helen 
Published: June 2022

Abstract
BackgroundAlmost one-half of women aged 45 years or over have been victims of intimate partner violence (IPV) during their lifetime, and this proportion is likely to increase as the population ages. Women impacted by IPV related to gambling face unique challenges, yet there is a paucity of research into older women’s past experiences of gambling-related IPV. This paper explores past experiences of older women who are victim-survivors of male partner violence linked to gambling. Exploring the generational differences (cohort effects) and wider contextual differences (period effects) in which gambling-related IPV occurred offers valuable insights for services who support women impacted by this issue, and into the role of gender inequality and gambling in IPV.MethodsQualitative interviews were undertaken with 72 women as part of a larger study exploring the nature of the relationship between problem gambling and IPV. The current study analysed a subset of 22 interviews with women aged 50 years or over. We analysed the data within a gender-sensitive socio-ecological framework using an adaptive grounded theory approach. Women’s experiences are analysed in terms of cohort and period effects on their past experiences of IPV.ResultsCohort effects on the women’s experiences of IPV included gendered attitudes, traditional views of marriage, silence surrounding IPV, reticence to disclose the abuse, and little understanding of problem gambling. Period effects included a lack of services for IPV and gambling, gendered service responses, and a failure of some service and institutional responses to prioritise the safety of victims. Three main areas of influence on the women’s experiences were apparent - gender inequality, tolerant attitudes towards and limited understanding of IPV, and the role of gambling in IPV. These influences reflect prevailing attitudes, systems and societal norms at the time of their victimisation. ConclusionThe findings point to the critical importance of reducing gender inequality to reduce male partner violence towards women. Women impacted by gambling-related IPV, including the legacy of past abuse, need effective service responses. Service providers must recognise the many forms and patterns of abuse and understand the historical and contextual factors that underlie and exacerbate it.
Fluorescence “Turn-Off” Sensing of Iron (III) Ions Utilizing Pyrazoline Based Sensor: Experimental and Computational Study
Authors: Sharma Promila , Bhogal Shikha , Mohiuddin Irshad , Yusuf Mohamad , Malik Ashok Kumar 
Published: June 2022

Abstract
A simple pyrazoline-based ‘‘turn off’’ fluorescent sensor 5-(4-methoxyphenyl)-3-(5-methylfuran-2-yl)-1-phenyl-4,5-dihydro-1H-pyrazole (PFM) was synthesized and well characterized by different techniques such as FT-IR, 1H-NMR, 13C-NMR, and mass spectrometry. The synthesized sensor PFM was utilized for the detection of Fe3+ ions. Fluorescence emission selectively quenched by Fe3+ ions compared to other metal ions (Mn2+, Al3+, Fe2+, Hg2+, Cu2+, Co2+, Ni2+, Cd2+, Pb2+, and Zn2+ ) via paramagnetic fluorescence quenching and showed good anti-interference ability over the existence of other tested metals. Under optimum conditions, the fluorescence intensity of sensor quenched by Fe3+ in the range of 0 to 3 μM with detection limit of 0.12 μM. Binding of Fe3+ ions to PFM solution was studied by fluorescent titration, revealed formation of 1:1 PFM-Fe metal complex and binding constant of complex was found to be of 1.3 × 105 M−1. Further, the fluorescent sensor has been potentially used for the detection of Fe3+ in environmental samples (river water, tap water, and sewage waste water) with satisfactory recovery values of 99-101%.
Computational Investigation of Isoquinoline Alkaloid from the Stems of Tinospora Cordifolia against Polycystic Ovarian Syndrome
Authors: Moka Murali Krishna , Sumithra M. 
Published: June 2022

Abstract
Polycystic Ovarian Syndrome (PCOS)is a global, substantial, metabolic, and endocrine health concern affecting one in every five women of progenitive aged (reproductive) women. Despite the fact that systematic screening and early diagnosis have improved the conception rate in recent decades, infertility still claims ~4%-20% of reproductive-aged women across the globe. In contemporary drug development, in-silico techniques are predominantly employed to investigate interactions between drugs and receptors, as well as quantum chemical properties. A computational approach was used in the current study to conform the quantum chemical properties, Density-functional theory (DFT) analysis, ADMET drug-likeness of the Isoquinoline, alkaloid [Berberine (BER), Palmatine (PAL), Jatrorrhizine (JAT), &Magnoflorine (MAG)], isolated from Tinospora cordifolia stem, and the four receptors were selected from PDB database.Methods Docking studies were carried out withHuman Androgen Receptor(1E3G),Insulin receptor(3EKK), Estrogen Receptor beta(1U3S),and Human steroidogenic cytochromeP450 17A1(6WR0)by using AutoDockVina with a pliable ligand docking approach. ADMET prediction and toxicological predictions were performed using SwissADME. DFT analysis was used to predict the optimized structures and molecular electrostatic potential surfaces of the isolated compounds, GROMACS for Molecular Dynamic (MD) Simulation Studies.Results and Discussion BER and PALshown better docking scores against the1E3Ghuman androgen receptor.The absorption of molecules through the skin was measured in cm/s by the skin permeability value (Kp). Higher negative values of MAG (-6.44), JAT (-5.9), PAL (-5.79), and BER (-5.78) resulted in lower skin permeation (log Kp, cm/s). CYP17A1 has a remarkable binding affinity for all four Isoquinoline alkaloids [BER (-5.46), JAT (-5.26), MAG (-6.13), and PAL (-6.01). Isoquinoline alkaloids (BER& PAL) have potential roles against various diseases, and in specific PCOS, scientific evaluation has been put forth based on virtual screening.
Tumor-derived Exosomal LncRNA SNHG16 Induces Telocytes to promote metastasis of hepatocellular carcinoma via miR-942-3p/MMP9 axis
Authors: Xu Ying , Luan Guangchao , Li Zhongchao , Liu Ziming 
Published: June 2022

Abstract
Background: Hepatocellular carcinoma(HCC) cells-derived exosomal LncRNA SNHG16(LncSNHG16) is highly expressed and associated with the poor overall survival of patients. Telocytes(TCs), as novel interstitial cells, possess the function to promote HCC metastasis. Therefore, whether a molecular interaction between exosomal LncSNHG16 and TCs will be revealed in our study.Methods: LncSNHG16 expression in HCC tissues and cell lines were detected, and its bioinformatics analysis was screened. Exosomes were isolated and purified from HCC cells with over-expressed/knockdown LncSNHG16 vectors and co-cultured with TCs. Then, the expression of the LncSNHG16/miR-942-3p/MMP9 axis was tested in TCs. Transwell and cell wound healing assays were designed to examine the invasion and migration of HCC cells after co-incubating with TCs. RNA immunoprecipitation(RIP) assay and Dual-Luciferase gene report were utilized to verify the binding effect of LncSNHG16, miR-942-3p, and MMP9 mRNA. In vivo, experimental animal models were established to confirm the effect of exosomal LncSNHG16-induced MMP9 expression in HCC metastasis.Results: Exosomal LncSNHG16 could be phagocytized by TCs and down-regulated miR-942-3p, which induced the targeted MMP9 up-regulation. LncSNHG16 had special binding sites with miR-942-3p in TCs to facilitate the migration of HCC cells in vitro and in vivo. Exosomal LncSNHG16 was finally supported as competing endogenous RNA of the miR-942-3p/MMP9 axis in TCs.Conclusion: Tumor-derived exosomal LncSNHG16 modulates MMP9 by competitively binding to miR-942-3p in TCs, thus promoting the metastasis of HCC.
Surface Morphology and Electrical Conductivity of In2S3 thin films Doped with Copper
Authors: Ebrahiminejad Zh. , Asgary S. , Masoudi S. F. , Ramezani A. H. 
Published: June 2022

Abstract
In the present work, the electrical properties and morphology of In2S3 thin films have been investigated. Also, the results have been carried out for In2S3 thin films doped with Cu. The effect of variation the temperature has been studied on the morphology of In2S3 thin films by using the AFM images. Thin films conductivity has been strongly influenced by Cu doping content, and temperature changing. Also, the electrical properties of these samples have been considered. Also, these properties have been analyzed for In2S3 thin films doped with Cu at the specific temperature. The results show that In2S3 thin films at lowest temperature has the maximum scattering component of transmission probability. Also, effect of different values of Cu () which has been doped in In2S3 thin films have been studied, as it increased, the transmission probability and current density of these thin films have been increased.
Taxonomy, Phylogeny and seasonal variation of endophytic fungi isolated from the traditional medicinal plant Madhuca neriifolia from the Western Ghats of Karnataka, India
Authors: Boruah Mamukan , Mahmood Riaz 
Published: June 2022

Abstract
Traditionally, the plant, Madhuca neriifolia is used for various disease treatments. Very few research reports about this plant from the South West of Western Ghats of Karnataka, India, are available. Further, no research reports about the possible existence of endophytic fungi inhabiting this plant are found in the literature. Compounds from endophytic fungi are crucial elements in curing diseases. Therefore, it is necessary to explore the endophytic fungi that may be inhabiting this plant. Hence, this study is conducted to isolate and identify the possible existence of endophytic fungi in this plant. The test results are found to be positive. Four different endophytic fungi strains with different morphologies are isolated and identified from the leaves and twigs of this medicinal plant. The isolated fungi are identified based on the morphology of the fungal colony, PC R-amplified fungal internal transcribed spacer sequences (ITS) and by the phylogenetic analysis with the MEGA X software. All four different endophytic fungi are identified at the level of their species. They are Alternaria alternata, Trichoderma asperellum, Aspergillus stellatus and Cladosporium cladosporioides.
Residual cancer is a strong predictor of survival in T3 incidental gallbladder cancer
Authors: You Chuan , Xie Mengyi , Ling Meng , Yanga Facai , Qiu Yinghe , Li Jingdong 
Published: June 2022

Abstract
Background and PurposeIndex cholecystectomy is insufficient for curing T3 incidental gallbladder cancer (IGC), and once residual cancer (RC) is found, the prognosis is often poor. The purpose of this study was to investigate the effect of RC on the prognosis and the optimal choice of adjuvant therapy for R0 reresection patients with T3 IGC.MethodsWe retrospectively reviewed data from patients with T3 IGC who underwent radical reresection from January 2013 to December 2018. RC was defined as histologically proven cancer at reresection. Demographics and tumour treatment-related variables were analysed in correlation with RC and survival. Adjuvant (Adj) chemoradiotherapy (CRT) was correlated with overall survival (OS) and disease-free survival (DFS).ResultsOf the 167 patients with IGC who underwent surgery, 102 underwent radical extended resection. Thirty-two (31.4%) RCs were found. Hepatic side tumours (T3h) and both side tumours (T3h+T3p) were associated with the presence of RC. In multivariate analysis, RC and lymph node metastasis were independent prognostic factors for DFS and OS (P <0.05). RC was associated with a significantly shorter median OS (20 vs. 53 months; P<0.01) and DFS (11 vs. 40 months; P<0.001) despite R0 resection. For R0 reresection patients with RC and/or lymph node metastasis, Adj CRT significantly improved OS (P=0.024).ConclusionResidual cancer and lymphatic metastasis are important factors for the poor prognosis of T3 IGC despite R0 resection, and these patients should actively receive adjuvant therapy.
Multiparametric dual-time-point [18F]FDG-PET/MRI for lymph node staging in patients with untreated FIGO I/II cervical carcinoma
Authors: Weissinger Matthias , Kommoss Stefan , Jacoby Johann , Ursprung Stephan , Seith Ferdinand , Hoffmann Sascha , Nikolaou Konstantin , Brucker Sara Yvonne , Fougère Christian la , Dittmann Helmut 
Published: June 2022

Abstract
Purpose:[18F]FDG PET/MRI comes with limited sensitivity for initial detection of lymph node metastases (LNM) in early stage cervical carcinoma. Therefore, the aim of this prospective study was to investigate the additional value of multiparametric dual-time-point PET/MRI and to asses potential influencing factors for LNM detection.Methods: 63 patients underwent whole-body dual-time-point [18F]FDG-PET/MRI ≈60+90min p.i. (Biograph mMR®, Siemens Healthineers). 251 LN were evaluated visually by nuclear medicine and radiology expert, quantified multiparametrically and were correlated with histology. Multiparametric analysis was performed using a logistic regression-based malignancy score (MS).Results:Overall prevalence of LNM was 17.1% and dependent on tumor grade (Gx 1/6, G1:0/19, G2:18/106, G3:24/120). Grading of the primary tumor (G2/G3) had a significant impact on visual detection (sens: 8.3%/31%).Best single parameter for LNM detection was SUVavg, however with a significant loss of discriminatory power in G2 vs. G3 tumors (AUC: 0.673/ 0.901). For multiparametric analysis, the independent predictors “SUVavg, ∆SUVpeak (60 to 90 min p.i.), LN sphericity, ADC and histologic grade” were included in the MS. Application of the MS enhanced AUCs especially in G2 tumors (AUC: G2:0.769; G3:0.877) and improved accuracy for single LNM from 34.5% to 55.5% compared to the best univariate parameter SUVavg.Equated to the expert consensus, use of the malignancy score increased overall sensitivity from 31.0% to 79.3% (Youden optimum) with a moderate decrease in specificity from 98.3% to 75.6%.Conclusion:Low histologic grad (G2 vs. G3) hampers visual detectability of LNM in [18F]FDG-PET/MRI. Multiparametric evaluation of dual-time-point PET/MRI has the potential to improve accuracy compared to visual interpretation and enables sufficient N-staging also in G2 tumors.Trial registration:DRKS00014346. Registered April 4, 2018 - retrospectively registered,https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00014346
Suppression of Interferon Stimulated Genes Enhance Viral Replication of Seasonal Influenza A Virus through the JAK/STAT pathway
Authors: H Alfredo Jr. , Kakee Sosuke , Kageyama Seiji , Tsuneki-Tokunaga Akeno , Perdana Waldy , Akena Yui , Kanai Kyosuke 
Published: June 2022

Abstract
Background
In a previous study, we described the diverse growth capabilities of circulating seasonal influenza A virus (IAV) with low to high viral copies in vitro. In this study, we analyzed the cause of differences in growth capability by evaluating Interferon-β and antiviral interferon-stimulated genes (ISGs) expression.
Methods
A549 cells (3.0x105 cells) were inoculated with circulating seasonal IAV strains for 6 and 24 h. In cells inoculated for 6 h, IAV production was assessed by IAV-RNA copies in culture supernatant and cell pellets for gene expression evaluation. At 24 hours post-infection, cells were collected for IFN-β and ISG-15 protein expression. JAK/STAT pathway inhibitor, pyridone 6 (P6) was used to evaluate the influence of ISGs on the growth capability of seasonal IAV.
Results
A549 cells inoculated with seasonal IAV strains with high growth capability expressed less IFN-β and ISGs, whereas low growth capability strains expressed more IFN-β and ISGs. The use of a P6, a JAK/STAT inhibitor, suppressed the expression of ISGs and enhanced the viral copies of seasonal IAV strains with low growth capability. This result suggests that growth capability is regulated by ISG-mediated IFN-β production.
Conclusion
In this study, we provide a new insight into the mechanism of circulating seasonal IAV replication. Our study showed that the difference in the growth capability of circulating seasonal IAV strains is due to the regulation of antiviral ISGs. Inhibition of the JAK/STAT pathway permits seasonal IAV strains with low growth capability to enhance their viral copies by suppressing antiviral ISGs. Our results may contribute in developing new effective therapeutic strategies and reduce the risk of developing severe influenza disease.
The weight distributions of several classes of few-weight linear codes
Authors: Sun Huan , Yue Qin , Jia Xue 
Published: June 2022

Abstract
In data storage systems, authentication codes, association schemes, and some other fields, linear codes with few weights play an important role.In this paper, we construct four classes of few weights linear codes over $\mathbb F_{q}$ and use Gauss periods in the semi-primitive case to determine their weight distributions.There are some linear codes with two weights whose duals are almost Maximum Distance Separable (AMDS) codes and a class of Maximum Distance Separable (MDS) codes.