bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management of Ascites Following Deceased Donor Liver Transplantation: A Case Series
Authors: Al-Zoubi Mohammad , Alarabiyat Moath , Hann Angus , Mehrzhad Homoyon , Karkhanis Salil , Muiesan Paolo , Abradelo Manuel , Hartog Hermien , Roberts Keith , Mirza Darius F. , Isaac John R. , Dasari Bobby V.M. 
Published: July 2022

Background.
Persistent ascites after orthotropic liver transplantation has numerous causes and can be challenging to manage. This study aimed to determine the outcomes associated with conservative and endovascular intervention of posttransplant ascites after deceased donor liver transplantation.

Methods.
Adult (≥18 y) liver transplant recipients (between 2006 and 2019) who underwent hepatic venous pressure studies to investigate posttransplant ascites were included in this retrospective study. Comparisons were made between those who were managed with conservative therapy versus endovascular intervention and were also based on hepatic venous wedge pressure gradient (normal [≤10 mm Hg] versus elevated [>10 mm Hg]).

Results.
A total of 30 patients underwent hepatic venography to investigate ascites during the study period. The median time from transplant to venography was 70 d. At least 1 endovascular intervention was performed in 18 of 30 patients (62%), and 12 of 30 patients (38%) were managed conservatively. Endovascular interventions included angioplasty (n = 4), hepatic vein stenting (n = 9), or a transjugular intrahepatic portosystemic shunt (n = 7). The mean (range) hepatic venous wedge pressure gradient for the conservative and endovascular intervention groups was 12 mm Hg (3–23) and14 mm Hg (2–35), respectively. At a 6-mo follow-up, ascites resolved in 6 of 12 patients (50%) and 11 of 18 patients (61%) in the medical management and endovascular groups, respectively. The graft survival rates at 6 and 12 mo were (7/12 [58%] versus 17/18 [94%], P = 0.02) and (7/12 [58%] versus 14/18 [78%], P = 0.25), respectively.

Conclusions.
Despite medical or endovascular intervention, resolution of ascites is achieved in <60% of patients with persistent ascites. Biopsy findings and venographic pressure studies should be carefully integrated into the management of posttransplant ascites.
Neural substrates of substance use disorders
Authors: Paulus Martin P. 
Published: July 2022

Purpose of review
Substance use disorders account for a tremendous burden to society, yet despite substantial progress in basic studies, our understanding of the brain-basis of these disorders is still emerging. This review summarizes the recent findings of neuroimaging studies with substance use disorder individuals.

Recent findings
Resting-state functional connectivity studies support for some but not all substances of abuse and disruption in executive control. Structural neuroimaging findings point towards reduced subcortical volumes, which may emerge as an interaction between preexisting factors and recent substance use. Longitudinal studies implicate some of the same core brain structures and their functional role that have also been identified via case–control studies. Finally, meta-analyses support the idea of dysregulation of cortical control over subcortical salience processing.

Summary
Although progress has been made and there is both structural and functional imaging evidence of an imbalance between brain structures involved in executive control and salience processing, there is emerging evidence that brain-behaviour relationships, which are core to discovering the neural processes that lead to and maintain substance use, are small and require larger consortia that prospectively examine individuals with substance use disorder.
Reproducibility and replicability in neuroimaging data analysis
Authors: Adali Tü̈lay , Calhoun Vince D. 
Published: July 2022

Purpose of review
Machine learning solutions are being increasingly used in the analysis of neuroimaging (NI) data, and as a result, there is an increase in the emphasis of the reproducibility and replicability of these data-driven solutions. Although this is a very positive trend, related terminology is often not properly defined, and more importantly, (computational) reproducibility that refers to obtaining consistent results using the same data and the same code is often disregarded.

Recent findings
We review the findings of a recent paper on the topic along with other relevant literature, and present two examples that demonstrate the importance of accounting for reproducibility in widely used software for NI data.

Summary
We note that reproducibility should be a first step in all NI data analyses including those focusing on replicability, and introduce available solutions for assessing reproducibility. We add the cautionary remark that when not taken into account, lack of reproducibility can significantly bias all subsequent analysis stages.
How Americans encounter guns: Mixed methods content analysis of YouTube and internet search data
Authors: Ranney Megan L. , Conrey Frederica R. , Perkinson Leah , Friedhoff Stefanie , Smith Rory , Wardle Claire 
Published: July 2022

AbstractFirearm-related injury and death is a serious public health issue in the U.S. As more Americans consume news and media online, there is growing interest in using these channels to prevent firearm-related harms. Understanding the firearm-related narratives to which consumers are exposed is foundational to this work.This research used the browsing behavior of a representative sample of American adults to identify seven naturally occurring firearm-related content ecosystems on YouTube, and the demographics and internet search patterns of users affiliated with each ecosystem.Over the 9-month study period, 72,205 panelists had 16,803,075 person-video encounters with 7,274,093 videos. Among these, 282,419 videos were related to firearms. Using fast greedy clustering, we partitioned users and channel interactions into seven distinct channel-based content ecosystems that reached more than 1/1000 YouTube users per day. These ecosystems were diverse in reach, users, and content (e.g., guns for self-protection vs. guns for fun).On average, 0.5% of panelists performed a firearm-related internet search on a given day. The vast majority of these searches were related to mass shootings or police-involved shootings (e.g., “active shooter”), and virtually none were about more common firearm harm such as suicide. Searches for firearm safety information were most common among panelists affiliated with the “Hunting & Fishing” and “Guns & Gear” ecosystems, which were watched primarily by older, white men.These findings identify an opportunity for analyzing firearm-related narratives and tailoring firearm safety messaging for users affiliated with specific online content ecosystems.HighlightsWe used a mixed methods network analysis of YouTube users’ viewing patterns and identified seven distinct firearm-related content ecosystems.Ecosystems vary by reach, audience demographics, and audience firearm-related search patterns.The ecosystems contain a wide variety of narratives about firearm use, safety, and potential harms.Understanding the diverse narratives across these ecosystems, their respective audiences and audience search patterns can inform future work to reduce firearm-related harms and increase safety.
Perfusable Apparatus For Thick-tissue Creation And Growth (patch) Of Cardiac Tissue
Authors: Pitaktong Isaree , He Yusheng Jason , Nurminsky Katherine , Dunn Tyler , Mir Amatullah , Koljaka Sarah , Dunne Olivia , Ran Stephanie , Shih Wesley , Wang Anya , Matsushita Hiroshi , Rodgers Daniel , Hibino Narutoshi 
Published: July 2022

AbstractCardiac tissue engineering has been developed as a potential alternative treatment for heart failure. However, current 3D tissues are limited in size and thickness due to the lack of an effective vascularization method. We have developed a novel bioreactor system to create viable vascularized cardiac tissue from multicellular spheroids using a digital light processing (DLP) 3D bioprinting system. Spheroids were created from induced pluripotent stem cells (iPSC) and cardiac fibroblasts (FB) using special dimple plates for mass production. One centimeter cubic tissues were created from spheroids using a DLP 3D printed mold with vascular channels. The tissue was maintained in a perfusion chamber under regulated flow and pressure following differentiation to cardiac tissue and endothelialization. Mass production of large spheroids (35,000 / tissue, diameter of 395.99 um +/- 101.15 um) was achieved from 170 million iPSCs and 50 million FBs for the creation of 1cm3 cardiac tissue in a 3D printed mold with vascular channels. The cardiac tissues (n=5) were perfused for 20 days under stable pressure of 17.5 +/- 3.05 PSI and flow of 5000 uL/min +/- 1116.42 uL/min. On days 10 and 20, Alamar blue assays showed viability for all five tissues (Alamar blue intensity: Day 10 1.57 +/- 0.15. Day 20 2.21 +/- 0.19). Thick and viable cardiac tissues were created and maintained using a 3D printed vascularized mold and perfusion system for maturation and growth in vitro for 30 days. This technology will open new doors for viable in vitro cardiac tissue creation.
The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variation
Authors: Narita Takeo , Higashijima Yoshiki , Kilic Sinan , Maskey Elina , Neumann Katrin , Choudhary Chunaram 
Published: July 2022

AbstractCis-regulatory enhancers are essential for differential expression of developmental and housekeeping genes. However, the specificity of native mammalian enhancers and how it shapes cell-type-specific gene expression landscapes remain largely unknown. We show that endogenous enhancers are broadly compatible with the promoters of developmental and housekeeping genes. Broad enhancer compatibility affords ‘retrofitting’ new regulatory capabilities to housekeeping genes that evolved before the advent of enhancers. This enables cell-type-specific tuning of ubiquitously expressed genes. Segregation between enhancer-dependent and –independent type regulation is blurred. Within the same cell type, a single promoter can be activated by enhancers and non-enhancer promoter-regulatory elements (PREs). It is the tunable and integrated strengths of enhancers and PREs that quantitatively shape gene expression landscapes, within and across cell types. Our findings have broad implications for understanding cell-type-specific quantitative gene expression variation, as well as the emergence and rewiring of gene regulatory networks in disease and organismal evolution.
How to scale up from animal movement decisions to spatio-temporal patterns: an approach via step selection
Authors: Potts Jonathan R. , Börger Luca 
Published: July 2022

AbstractUncovering the mechanisms behind animal space use patterns is of vital importance for predictive ecology, thus conservation and management of ecosystems. Movement is a core driver of those patterns so understanding how movement mechanisms give rise to space use patterns has become an increasingly active area of research.This study focuses on a particular strand of research in this area, based around step selection analysis (SSA). SSA is a popular way of inferring drivers of movement decisions, but, perhaps less well-appreciated, it also parametrises a model of animal movement. Of key interest is that this model can be propogated forwards in time to predict the space use patterns over broader spatial and temporal scales than those that pertain to the proximate movement decisions of animals.Here, we provide a guide for understanding and using the various existing techniques for scaling-up step selection models to predict broad scale space use patterns. We give practical guidance on when to use which technique, as well as specific examples together with code inRandPython.By pulling together various disparate techniques into one place, and providing code and instructions in simple examples, we hope to highlight the importance of these techniques and make them accessible to a wider range of ecologists, ultimately helping expand the usefulness of step selection analysis.
Discovering triple negative breast cancers (TNBC) transcriptomic profiles via massive screening of single cell RNA-sequencing (scRNA-seq) datasets
Authors: Rafsanjani Mohd Rifqi , Onn Muhammad Asyraf , Zakaria Norashikin 
Published: July 2022

ABSTRACTTriple negative breast cancers (TNBC) account for 18% of the breast cancer patients in Malaysia and also account for one of the worst prognoses albeit it is the rare cancer types. TNBC is defined as breast cancers that tested negative on all 3 tests of oestrogen/progesterone (ER/PR) receptor and human epidermal growth receptor 2 (HER2). Understanding the TNBC biology is challenging due to the fact of multiple subtypes and its heterogeneity in patient samples. Here we attempt to examine the transcriptional profiling of TNBC using the single cell RNA-sequencing (scRNA-seq) analysis of different publicly available TNBC datasets. In comparison to bulk-RNA, scRNA-seq allowed the mapping of transcriptional profiling of TNBC at the single cell resolution and able to accurately pinpoint pan-transcriptional markers of TNBC.
A home-cage, video monitoring-based mouse frailty index detects age-associated morbidity in the absence of handler-induced stress
Authors: Ruby J. Graham , Ylagan Paulo , Di Francesco Andrea , Zavala-Solorio José , Keyser Robert , Williams Owen , Spock Sarah , Li Wenzhou , Vongtharangsy Nalien , Chatterjee Sandip , Sloan Cricket A. , Ledogar Charles , Kuiper Veronica , Kite Janessa , Cosino Marcelo , Cha Paulyn , Karlsson Eleanor M. 
Published: July 2022

AbstractFrailty indexes provide quantitative measurements of non-specific health decline and are particularly useful as longitudinal monitors of pre-mortal morbidity in aging studies. For mouse studies, frailty assessments can be taken non-invasively, but they require handling and direct observation that is labor-intensive to the scientist and stress-inducing to the animal. Here, we implement, evaluate, and provide a digital frailty index composed entirely of computational analyses of home-cage video and compare it to manually obtained frailty scores in genetically diverse mice. We show that the frailty scores assigned by our digital index correlate with both manually obtained frailty scores and chronological age. Thus, we provide a tool for frailty assessment that reduces stress to the animal and can be collected consistently, at scale, without substantial labor cost.
Antibiotic induced biofilm formations in Pseudomonas aeruginosa strains KPW.1-S1 and HRW.1-S3 are associated with increased production of eDNA and exoproteins, increased ROS generation, and increased cell surface hydrophobicity
Authors: Kumar Abhinash , Saha Saurav K , Banerjee Paromita , Sengupta Tapas K 
Published: July 2022

AbstractPseudomonas aeruginosa is a medically important bacteria due to its ability to form biofilm and is also an opportunistic pathogen. Pseudomonas aeruginosa has the intrinsic ability to form biofilm as one of the defense mechanisms for their survival. The fact that it can form biofilms on various medical implants makes it more harmful clinically. Although various antibiotics are used to treat Pseudomonas aeruginosa infections, previous studies have shown that sub-MIC levels of antibiotics cause biofilm formation in this type of bacteria. The present study thus deals with the effect of the aminoglycoside antibiotic gentamicin on the biofilm dynamics of two Pseudomonas aeruginosa strains KPW.1-S1 and HRW.1-S3. Biofilm formation was seen to be increasing with increased gentamicin concentrations in growth media. Confocal laser scanning microscopy and scanning electron microscopy accompanied with other biochemical tests deduced that biofilm-forming components like exoproteins, eDNA, and exolipids as exopolymeric substances in Pseudomonas aeruginosa biofilms were increased in the presence of gentamicin. An increase in reactive oxygen species generation along with increased cell surface hydrophobicity was also seen in both strains when treated with gentamicin. The observed increase in the adherence of the cells accompanied by an increase in exopolymeric substances, eDNA, and exolipids may have largely contributed to the increased biofilm production by the Pseudomonas aeruginosa strains KPW.1-S1 and HRW.1-S3 under the stress of the antibiotic treatment.
Multi-omics reveal Salmonella-liberated dietary L-arabinose promotes expansion in superspreaders
Authors: Ruddle Sarah , Massis Liliana M. , Cutter Alyssa C. , Monack Denise M. 
Published: July 2022

SUMMARYOur understanding of specific metabolites that drive host-pathogen interactions in the guts of superspreader hosts is incomplete. In a mouse model of chronic, asymptomatic Salmonella enterica serovar Typhimurium (S. Tm) infection, we performed untargeted metabolomics on the feces of mice and found superspreader hosts possess distinct metabolic signatures compared to non-superspreaders, including differential levels of L-arabinose. RNA-seq on S. Tm in superspreader fecal samples showed increased expression of the L-arabinose catabolism pathway in vivo. By combining bacterial genetics and diet manipulation, we demonstrate that diet-derived L-arabinose provides S. Tm a competitive advantage in the gut. We show that expansion of S. Tm in the gut requires a previously uncharacterized alpha-N-arabinofuranosidase that can liberate L-arabinose from dietary polysaccharides. Ultimately, we demonstrate that pathogen-liberated L-arabinose from the diet provides a competitive advantage to S. Tm in vivo. These findings propose L-arabinose as a critical driver of S. Tm expansion in the guts of superspreader hosts.
Factors impacting acceptance of e-learning in India: learners' perspective
Authors: Duggal Sanya 
Published: July 2022

PurposeThis study aims to identify the most significant factors that influence acceptance of e-learning in India. As e-learning has gained popularity in the wake of the COVID-19 pandemic and continues to be one of the most sustainable methods of education, it is pertinent to examine learners' perception towards its acceptance. There is limited literature available on this subject in India, especially factoring in impact of the pandemic.Design/methodology/approachThis study empirically analyses data of 331 adult e-learners in India, who have enrolled for one of the following e-learning formats: higher education, private coaching, test preparation, re-skilling and online certifications, corporate training and hobby and language-related learning. Their perception is examined on the basis of a model developed using the Unified Theory of Acceptance and Use of Technology model. Data are analysed through structural equation modelling using SPSS and AMOS statistical tools.FindingsThe result of the study shows that Infrastructure Dependability, Effectiveness of Design and Content of Courses and Student's Competency with Computers are the top three factors impacting e-learning acceptance in India.Research limitations/implicationsThis study makes several theoretical contributions. Additionally, research findings and recommendations will facilitate education providers, corporates in the education industry and policymakers to focus on the significant areas for enhancing the acceptance of e-learning.Originality/valueThis study identifies and confirms important factors that influence e-learning acceptance and suggests opportunities for further in-depth research and analysis.
Factors influencing consumer use of a sport-branded app: the technology acceptance model integrating app quality and perceived enjoyment
Authors: Won Doyeon , Chiu Weisheng , Byun Hyun 
Published: July 2022

PurposeThe current study extends the technology acceptance model (TAM) and information system success model (ISSM) to the context of branded sport applications. Specifically, the study examined the influences of app system success dimensions and TAM determinants on branded sport app usage intention. Moreover, the current study examined the gender differences regarding the relative importance of the drivers and predictors of usage intention.Design/methodology/approachData collection (n = 256) was conducted using convenience sampling in South Korea. The data were primarily analyzed via partial least squares structural equation modeling (PLS-SEM), multi-group analysis and importance–performance map analysis (IPMA) using SmartPLS 3.0.FindingsApp users who viewed branded sport apps as having a higher level of system and information quality were likely to have stronger perceptions of enjoyment, usefulness, and ease of use. Among the TAM determinants, perceived enjoyment most significantly influenced their usage intention, followed by perceived usefulness and perceived ease of use. Multigroup analysis revealed that some relationships between app system success dimensions and TAM determinants were significantly different by gender. In addition, IPMA indicated that perceived enjoyment and system quality of branded sport apps were relatively more important than the other predictors.Originality/valueThe current study contributes to the literature by incorporating both TAM and ISSM and extending the TAM with the perceived enjoyment construct to examine the key determinants of usage intention in the context of branded sport apps.
Participative climate, team job crafting and leaders' job crafting: a moderated mediation model of team performance
Authors: Khan Sadeeqa Riaz , Qammar Ahmad , Shafique Imran 
Published: July 2022

PurposeDespite increasing interest in team performance, particularly in team-based work environment, the literature is underdeveloped in explaining the mechanisms that lead to team performance. The purpose of this paper, a team-level research, is to advance the understanding of one of the mechanisms that explains how participative climate relates to team performance by studying the roles of team job crafting and leader job crafting in the process.Design/methodology/approachA moderated mediation model of team performance is tested through causal mediation analysis of the R mediation package using multi-sourced data collected from 250 team members working in 58 teams in the software development industry (about participative climate and team job crafting) and from their respective 58 team leaders (about leader job crafting and team performance) through online survey questionnaires.FindingsThe results indicate that teams with the perception of a higher level of participative climate are more likely to engage in team job crafting, which, in turn, leads to team performance. However, the data do not support the hypothesized moderating effect of the leader's job crafting.Practical implicationsThe findings offer implications for practitioners in designing policies that improve team job performance by better understanding one of the mechanisms through which team performance is improved. The study would potentially guide practitioners on the importance of developing a participating climate in job crafting and team performance.Originality/valueThis study not only contributes to the job crafting literature by introducing participative climate as one of the important antecedents to team job crafting, but also enriches the literature on team effectiveness by explaining and empirically demonstrating the role of team job crafting in the link between participative climate and team performance. To the best of our knowledge, the current study is the first one to conceptualize the role of leaders' job crafting in participative climate, team job crafting, and team performance relationships.