bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient Satisfaction, Perception-Expectation Gap, and Costumer Satisfaction Index in Annual Survey 2021 at Dr. Soetomo General Academic Hospital
Authors: Prakoeswa Cita Rosita Sigit , Hidayah Nur , Dewi Arlina , Purnamasari Indah , Adriansyah Agus Aan , Yaqub Amak M. 
Published: June 2022

Highlights:

Customers expectations at Dr. Soetomo General Academic Hospital has a higher quality of service.
The handling of complaints has a low performance on the quality of services at Dr. Dr. Soetomo General Academic Hospital.
Improving service quality requires improvements in efficient complaint handling.

Abstract:
Service quality is essential in health institutions that can affect patient satisfaction and loyalty. The growth in the number of patients at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, triggered the diversification of services that require periodic quality control. This study aimed to investigate the performance of 31 departments/units in the hospital, the level of customer satisfaction with services provided, and factors that could influence satisfaction. A survey about service performance, perception, and expectation of services was carried out in 31 departments/units using questionnaires from August to October 2021. Convenient respondents consisted of 2121 patients and their families aged >15. Questionnaire items assessed performance, perceptions, and expectations of health services using the SERVQUAL method. Statistical Package for the Social Sciences (SPSS) software was used to perform an analysis of the results of the measurement and the difference in responses between demographic groups of respondents (p<0.05 was significant). The mean performance score was 92.86, and the gap between their perception and expectation averaged -0.23. Customers' expectations of the provider's competence were met, and its performance was perceived to be the best. Meanwhile, handling complaints was perceived as having the lowest performance, while customers’ satisfaction over it had not been met. The results showed that the hospital could still not fully meet some of the customer expectations, with immediate improvements needed in handling complaints.
A First Step to Novel Approach for Treating Alkali Injury of the Cornea: Effect of Platelet Rich Fibrin Lysates on Cultured Rabbit (Oryctolagus cuniculus) Limbal Stem Cell Proliferation Exposed by Sodium Hydroxide
Authors: Prabawati Wahyu Endah , Suhendro Gatut , Retnowati Endang 
Published: June 2022

Highlights:

Sodium hydroxide causing platelet rich fibrin stimulates limbal stem cell proliferation in chemical trauma.
Limbal stem cell niche and influences limbal stemness was repaired by Platelet Rich Fibrin.

Abstract:
Chemical injuries of the eye produce extensive damage to the ocular surface and limbal stem cells, resulting in permanent unilateral or bilateral visual impairment. Alkali injuries occur more frequently than acid injuries. Platelets are a rich source of potential wound healing, promoting polypeptide growth factors. This study aimed to investigate the effect of platelet-rich fibrin (PRF) lysates on limbal stem cell proliferation, which was exposed to sodium hydroxide that resembled limbal stem cell deficiency due to chemical trauma. Confluent rabbit (Oryctolagus cuniculus) limbal stem cells wounded using 20µL of 0.00625 M sodium hydroxide (pH 13) were treated with platelet-rich fibrin lysates (PRF) (0, 5, and 10%). PRF lysates were prepared from peripheral rabbit blood according to Choukroun's method without using anticoagulant and foreign factors for platelet activation. The proliferation of limbal stem cells was measured by a 3-(4,5-dimethylthiazol-2-yl)-2.50 diphenyl tetrazolium bromide (MTT) colorimetric assay at 24, 48, and 72 hours after exposure to sodium hydroxide. Proliferation significantly increased limbal stem cells with PRF lysates 5% (p<0.01) and 10% (p<0.01) group compared with the control (PRF 0%). There was no significant difference between PRF lysates of 5% and 10% (p>0.01). The highest proliferation of limbal stem cells was found in the PRF lysates 5% group after 48 hours (100.24%). PRF stimulated limbal stem cell proliferation in chemical trauma caused by the sodium hydroxide model. PRF repaired the limbal stem cell niche and influenced the limbal stemness. The present findings warrant further research on PRF as a novel alternative treatment for limbal stem cell deficiency.
Research Advance on Prediction and Optimization for Fracture Propagation in Stimulated Unconventional Reservoirs
Authors: Huang Luoyi , Lu Mingjing , Sheng Guanglong , Gong Jie , Ruan Jiayu 
Published: June 2022

Abstract
Multistage stimulation horizontal wells are prerequisite technologies for efficient development of unconventional reservoir. However, the induced fracture network morphology from hydraulic fracturing is very complex and affected by many factors, such as the in situ stress, rock mechanical properties, and natural fracture distribution. The large numbers of natural fractures and strong reservoir heterogeneity in unconventional reservoirs result in enhanced complexity of induced fractures from hydraulic fracturing. Accurate description of fracture network morphology and the flow capacity in different fractures form an important basis for production forecasting, evaluation (or optimization) of stimulation design, and development plan optimization. This paper focuses on hydraulic fracturing in unconventional reservoirs and discusses the current research advances from four aspects: (1) the prediction of induced fracture propagation, (2) the simulation of fluid flow in complex fracture networks, (3) the inversion of fracture parameter (fracture porosity, fracture permeability, etc.), and (4) the optimization of hydraulic fracturing in unconventional reservoirs. In addition, this paper provides comparative analysis of the characteristics and shortcomings of the current research by outlining the key technical problems in the study of flow characterization, parameter inversion, and optimization methods for stimulation in unconventional reservoirs. This work can provide a certain guiding role for further research.
Barley root exudates collection and primary metabolite profiling
Authors: Escudero-Martinez Carmen , Foito Alexandre , Kapadia Rumana , Aprile Alessio , Bulgarelli Davide 
Published: June 2022

Abstract
Plant exudates are one of the main drivers for the proliferation of the rhizosphere microbiota. We developed a protocol to quantify primary metabolites in exudates of the global crop barley. We used 2- and 3-week-old barley plants grown in sterilised sand and their root exudates were collected for 6 hours in ddH2O water to avoid degradation/transformation of the compounds. These samples were used to determine total carbon and total nitrogen by Dumas. Finally, we profiled primary metabolites in collected exudates by gas chromatography/mass spectrometry (GC-MS).
A systematic review on the global burden of human monkeypox: epidemiology and implications for outbreaks
Authors: Bhattacharjee Priyadarshini , Sinha Ankit , Prosad Ipshita 
Published: June 2022

Abstract
Monkeypox is a viral zoonosis having symptomatology that is likened to those observed in smallpox patients. More than 120 confirmed or suspected cases of monkeypox have been reported in at least 11 non-African countries in the past week. The emergence of the virus in separate populations across the world where it doesn’t usually appear has alarmed scientists and sent them racing for answers.The disease is clinically lesser in severity than smallpox, but still has a mortality rate of about 10%. Since the eradication of smallpox and cessation of smallpox vaccination, monkeypox is currently the most relevant orthopoxvirus in humans, especially in 2022. Monkeypox occurs mostly in Central and West Africa, but small outbreaks have also been observed in e.g. the US in 2003. A new third-generation vaccine has been approved to prevent this disease.
Using MetaboAnalyst 5.0 Part I: Optimizing parameters for LC-HRMS spectra processing
Authors: Pang Zhiqiang , Zhou Guangyan , Ewald Jessica , Chang Le , Hacariz Orcun , Basu Niladri , Xia Jianguo 
Published: June 2022

Abstract
Liquid-chromatography coupled with high-resolution mass spectrometry (LC-HRMS) has become a workhorse in global metabolomics studies with growing applications across biomedical and environmental sciences. However, outstanding bioinformatics challenges in terms of data processing, statistical analysis and functional interpretation remain critical barriers to the wider adoption of this technology. To help the user community overcome these barriers, we have made major updates to the well-established MetaboAnalyst platform (www.metaboanalyst.ca). This protocol is part one of four and provides stepwise instructions on how to use MetaboAnalyst to optimize parameters for LC-HRMS spectra processing. It may take ~2 hours to complete depending on the server load.
Using MetaboAnalyst 5.0 Part III: Multi-omics integration
Authors: Pang Zhiqiang , Zhou Guangyan , Ewald Jessica , Chang Le , Hacariz Orcun , Basu Niladri , Xia Jianguo 
Published: June 2022

Abstract
Liquid-chromatography coupled with high-resolution mass spectrometry (LC-HRMS) has become a workhorse in global metabolomics studies with growing applications across biomedical and environmental sciences. However, outstanding bioinformatics challenges in terms of data processing, statistical analysis and functional interpretation remain critical barriers to the wider adoption of this technology. To help the user community overcome these barriers, we have made major updates to the well-established MetaboAnalyst platform (www.metaboanalyst.ca). This protocol is part three of four and provides stepwise instructions on how to use MetaboAnalyst to integrate metabolomics data with transcriptomics data or to combine multiple metabolomics datasets.
The global publication trend and research development of nerve block: A visualisation analysis between 1997 and 2021
Authors: Dong Xiang , Zhao Pengcheng , Zhao Hui , Liu Jinxing , Chen Jie 
Published: June 2022

Abstract
Background: Nerve block is a clinical technique for relieving pain during surgery as well as for postoperative analgesia and chronic pain. Here, we analysed the trends in nerve block research and compared the contributions from different countries, institutions, journals, and authors. Methods: We extracted all studies concerning nerve block published between 1997 and 2021 from the Web of Science database. Microsoft Excel and VOSviewer were used to collect publication data, analyse publication trends, and visualize relevant results. Results: A total of 6,437 publications with 111,612 citations were identified between 1997 and 2021. The United States contributed the greatest number of publications (2010), whereas China ranked second (518). Regional Anesthesia & Pain Medicine had published the most papers concerning nerve block. Harvard University and Mariano ER were the most productive institution and author, respectively, in the field. Keywords were categorized into five clusters: the association between nerve block and anaesthesia or analgesia, local anaesthetic drugs for nerve block, nerve block techniques, nerve block for postoperative analgesia, and nerve block for oral local anaesthesia. Management-related research was a heavily researched topic in the field. Conclusions: We concluded that the United States contributed the most studies, and developing countries, led by China, are also encouraging study in this area. The exploration of new drugs in the fields of nerve block, multi-modal analgesia after surgery, treatment of chronic pain, analgesia for thoracic and abdominal surgeries, and analgesic interventions in oral therapy are a potential focus for future research.
Construction of a risk prediction model as an Independent Disease-Free Prognostic Indicator in Seminoma by Bioinformatic Analysis
Authors: Yu DI , Chunfa CHEN , Zhifeng YI 
Published: June 2022

Abstract
N6-Methyladenosine (m6A) are significant in directing different genes’ expression and their phenotype in different types of cancers. On the other hand, the function of m6A in seminomatous germ cell tumors (SGCT) is still unexplored. Our research attempts to demonstrate the disease-free prognosis value of m6A RNA methylation regulators in SGCT. The gene expression and accompanying clinical data of 65 SGCT tissues and 85 NSGCT tissues retrieved from the TCGA database were analyzed. The landscape of m6A RNA methylation regulators in SGCT was displayed. All SGCT patients were grouped into two clusters by consensus clustering of m6A RNA methylation regulators, and the clusters were connected to the disease-free prognosis and clinicopathological characteristics of SGCT(p = 0.015). Furthermore, the risk score(1.52YTHDC1 + 2.22HNRNPC-2.735WTAP) model was obtained and constructed by the univariate Cox regression analysis, and the least absolute shrinkage and selection operator (LASSO) Cox regression. The risk score was interrelated with stage status and laterality status of SGCT. Under the same conditions, the right side may have a higher risk score than the left one. More significantly, the risk score can serve as an self-supporting disease-free prognostic marker for SGCT patients. Therefore, m6A RNA methylation regulators also played an important role of SGCT in its disease-free survival prognosis.
Safety and efficacy in pediatric secondary intraocular lens implantation, in-the-bag versus sulcus fixation: a multicenter, single-blinded randomized controlled trial
Authors: Zou Yingshi , Jin Ling , Qu Bo , Chen Hui , Zeng Mingbing , Li Xia , Liu Xinhua , Luo LIxia , Liu Zhenzhen , Liu Yizhi 
Published: June 2022

Abstract
Background: Treatment of pediatric cataract remains challenging because of extremely high incidence of postoperative adverse events (AEs), especially, the AEs related to the locations of secondary implanted intraocular lens (IOL). There are two common locations for secondary IOL implantation in pediatric aphakic eyes: ciliary sulcus or in-the-bag implantation. However, there are currently no large, prospective, studies comparing complication rates and visual prognosis of in-the-bag versus ciliary-sulcus secondary IOL implantation in pediatric patients. Whether or how much secondary in-the-bag IOL implantation benefits the pediatric patients more than sulcus implantation and deserves to be performed routinely by surgeons remain to be elucidated. Here we describe the protocol of a randomized controlled trial (RCT) designed to evaluate the safety and efficacy of two approaches of IOL implantation in pediatric aphakia. Methods: The study is a multicenter, single-blinded RCT. Overall, a minimum of 284 eyes (approximately 228 participants assuming 75% have two study eyes) will be recruited. This study will be carried out in four eye clinics across China. Consecutive eligible patients are randomized to undergo either: secondary in-the-bag IOL fixation or secondary sulcus IOL fixation. Participants with two eyes eligible will receive the same treatment. The primary outcome is the incidence of glaucoma-related AEs by 1-year postoperatively. The secondary outcomes include incidence of other AEs, IOL decentration and tilt, visual acuity and ocular refractive power. Analysis of the primary and secondary outcomes is to be based on the intention­to­treat principle. Statistical analyses will includeχ 2 test or Fisher’s exact test for the primary outcome; Mixed model and GEE (Generalized estimated equation) model for the secondary outcome; Kaplan-Meier survival curves for the cumulative probability of glaucoma-related AEs over time in each group. Discussion: To the best of our knowledge, this study is the first RCT to evaluate the safety and efficacy of secondary IOL implantation in pediatric aphakia. The results will provide high-quality evidence for the clinical guidelines of the treatment of pediatric aphakia. Trial registration: ClinicalTrials.gov, NCT05136950. Registered on 1 November 2021
Effects of an early multidisciplinary intervention on sickness absence in patients with persistent low back pain - a randomized controlled trial
Authors: Fisker Annette , Langberg Henning , Petersen Tom , Mortensen Ole Steen 
Published: June 2022

Abstract
BackgroundMultidisciplinary rehabilitation is recommended to reduce sickness absence and disability in patients with subacute or chronic LBP. This RCT aimed to investigate whether a 12-week coordinated work oriented multidisciplinary rehabilitation intervention was effective on return to work and number of days off work during one-year follow-up when compared to usual care. MethodsThis study is a randomized controlled trial comparing a 12-week multidisciplinary vocational rehabilitation program with usual treatment. 770 patients with LBP, who were sick-listed, or at risk of being sick-listed were included in the study. The primary outcome was number of days off work due to LBP. The secondary outcomes were disability, health-related quality of life, pain, psychological distress and fear avoidance behavior.Data were collected at baseline, at the end of treatment, and at 6- and 12-months follow-up. Analyses were carried out according to the “intention-to-treat” principles.Results A significant decrease in the number of days off work was found in both groups at the end of treatment and at 6- and 12-months follow-up. Additionally, disability, pain, health related quality of life, psychological distress, and fear avoidance beliefs improved in both groups. No statistically significant differences were found between the groups on any of the outcomes.Conclusions The coordinated multidisciplinary intervention had no additional effect on sickness absence, disability, pain, or health related quality of life as compared with that of usual care. Trial registrationThis study was registered in ClinicalTrials.gov (registration ID: NCT01690234). The study was approved by The Danish Regional Ethics Committee (file no: H-C-2008-112) as well as registered at and approved by the Danish Data Protection Agency.
Antimicrobial Activities of Secondary Metabolites from Airborne Cladosporium Species Isolated in Cairo, Egypt
Authors: Abdulrahman Mohammed S. , Mansy Moselhy S. , Mekawey Amal A. I. , hefny Hamido M. , Amin Basma H. 
Published: June 2022

Abstract
Background
The antibiotic resistance of bacterial pathogens has become a serious health concern and encourages the search for novel and efficient antimicrobial metabolites. There has been a tremendous increase in interest in screening plants and fungi as a source for compounds with antimicrobial activities.
Objectives
This current study aimed to determine the possible antimicrobial activity of secondary metabolites of the Cladosporium species against some bacteria (Gram positive and Gram negative) and fungi.
Method
in the current work, Cladosporium were isolated from air in Cairo by using settle plate method. The samples were purified and identified by using macroscopical and microscopical methods, with the aid of biochemical reactions. Antimicrobial activity was performed by using agar well diffusion method. Those with antimicrobial activity subjected to submerged fermentation and metabolites were extracted using ethyl acetate. Extracts are separated by Chromatographic methods, nature of active metabolites was identified by High Performance Liquid Chromatography-Diaiod Array Aided Detector (HPLC-DAD) analysis. The mechanism of antimicrobial activity then investigated by Scan Electron Microscopy (SEM) and Transmission Electron (TEM) Microscopy.
Results
the current investigation revealed that 5.6% of Cladosporium extracts showed marked antimicrobial activity specially that from C. cladosporioides, C. herbarum and C. cladosporioides species, in addition, by using HPLC-DAD technique revealed the presence of compounds which may be responsible for the antimicrobial activities they elicit, including acropyrone, nigricinol, cladosporin, coumarin, citreoisocoumarin and questinol as major components may be responsible for antimicrobial activity. Scan electron microscopy (SEM) and transmission electron microscopy (TEM) revealed the effect of these compounds on outer surface of tested microorganism.
Conclusion
Cladosporium species could be a promising source of antimicrobial compounds with pharmaceutical and industrial importance.
Teaching Behaviors in Dynamic Geometry Environment: An Investigation of Nine Chinese Mathematics Lessons in The Prospective of Instrumental Orchestration
Authors: ZHU Fangchun , Binyan Xu 
Published: June 2022

Abstract
This paper focuses on how Chinese mathematics teachers integrate dynamic geometry software in lessons. Instrumental Orchestration (IO) lens and its categorization are used to analyze 9 Chinese mathematics lessons. As a result, it shows that most orchestration types can be identified in this study with some different characteristics observed. And a more classic way is identified for Chinese teachers to use DGS which indicates DGS is assimilated into the current teaching process. Teachers more use dragging mode to show the initial and final state of the figures. We also re-organize IO types and divided explain-the-screen into T-explain-the-screen and S-explain-the-screen based on who can be the explainer. This indicates that these teachers try to change their teaching process from lecturing to facilitating student’s learning.
The Effect of Gross Domestic Product, Urbanization, Trade Openness, Financial Development and Renewable energy on CO2 Emission
Authors: Suhrab Muhammad , Soomro Jahangeer Ahmed , Ullah Saif , Chavara Javeed 
Published: June 2022

Abstract
Pakistan is among those countries where the standards for environmentally friendly energy sources haven't complied as they should be. This investigation aims that CO2 emission is a dilemma for the modern world. It harms the climate and other aspects like the ozone layer, so we've incorporated certain variables to figure a way to reduce CO2 emissions. Data collection: The analysis was performed on E-views, and the World Bank's website has been used for data extraction. The data incorporated was from the year 1985 till 2018. Different statistical tools were applied in this research to overcome biases in data results further all such methods were applied which have common reliability on their results, the stationary test applied to check the level of stationary and results are satisfied on leg II of the stationary test, another most common used method regression applied and results of the regression are also satisfied with the hypothesis of the study, cointegration results found long term relation among variables and granger causality applied for cause and effects measurement. Findings: the results of the study revealed that Renewable Energy has a significant and negative effect on Carbon dioxide emissions. At the same time, Gross Domestic Product (growth) builds an insignificant and negative relationship with Carbon dioxide emissions. Meanwhile, the Financial Development (private sector), Trade Openness, and Urban Population have a significant and positive relation with Carbon Dioxide. Originality/value: This research contains an insight for the government officials that reveals what the CO2 emission is derived from and how it could be reduced. The recommendations have been provided for consideration as well.