bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Novel and Known LDLR Variants Triggering Severe
Familial Hypercholesterolemia in Saudi Families
Authors: Alnouri Fahad , Al-Allaf Faisal A. , Athar Mohammad , Al-Rasadi Khalid , Alammari Dalal , Alanazi Menwar , Abduljaleel Zainularifeen , Awan Zuhier , Bouazzaoui Abdellatif , Dairi Ghida , Elbjeirami Wafa M , Karra Hussam , Kinsara Abdulhalim J , Taher Mohiuddin M. 
Published: March 2022

Background:
Familial hypercholesterolemia (FH) is a common illness mainly caused by
variants occurring in the low-density lipoprotein receptor (LDLR) gene. FH is a leading cause of coronary
artery disease.

Objective:
This study aims to determine genetic defect(s) in homozygous and heterozygous FH index
patients and their first-degree blood relatives and understand the genotype-phenotype correlation.

Methods:
This study employed the genetic screening of FH-related genes by next-generation sequencing
and cascade screening by capillary sequencing.

Results:
We identified the presence of a novel frameshift variant [c.335_336insCGAG,
p.(F114Rfs*17)] and three known missense variants [c.622G>A, p.(E208K)], [c.1474G>A, p.(D492N)],
[c.1429G>A, p.(D477N)] in the LDLR gene of four unrelated Saudi families with FH. In proband 1, a
nonsense variant c.1421C>G, p.(S474*) was also detected at exon 9 of the lipoprotein lipase gene. The
segregation arrangement of the identified variants corresponded with the clinical characteristics. In this
study, all the detected variants were confined in the ligand-binding domain and epidermal growth factor
(EGF)-precursor homology domain of the LDLR protein, which portrayed severe clinical phenotypes of
FH. Moreover, these LDLR variants were in a highly conserved residue of the proteins.

Conclusion:
In addition to the finding of the novel variant in the LDLR gene that extends the spectrum
of variants causing FH, the results of this study also support the need for diagnostic screening and cascade
genetic testing of this high-risk condition and to understand the genotype-phenotype correlation,
which could lead to better prevention of coronary artery disease.
A Motif in Metallopeptidase Inhibitor Decreases Effectively the Activity of
Macrophage Metalloproteinases
Authors: Najafi Mohammad , Esfandiari Golnaz , Ghasempour Ghasem , Kakavandi Naser , Soleimani Ali Akbar , Abkenar Borhan Rahimi , Bahraini Elham , Khosravi Mohsen 
Published: March 2022

Objective:
The tissue remodeling process and cellular migration relate to the activities of
matrix metalloproteinases (MMPs). The aim of this study was to investigate the effects of a predicted
motif from TIMPs on the MMP-2 and MMP-9 activities secreted from the differentiated macrophages.

Methods and Materials:
The monocytes were isolated from the healthy individuals by RosetteSep
kit and were differentiated into macrophages using M-CSF. A 4-amino acid motif (TCAP) was predicted
using bioinformatics tools. Zymography technique was applied for the measurement of MMP
activities. The docking studies were also investigated between MMPs, tetrapeptide, and Batimastat.

Results:
The TCAP inhibited significantly the differentiated macrophage MMP-2 and MMP-9 activities
(p=0.0001and p=0.01, respectively). The docking results suggested that some MMP amino acids
are involved with both tetrapeptide (TCAP) and Batimastat,

Conclution:
The data showed that the small motif (TCAP) of TIMPs inhibits effectively the MMP-
2 activity.
Exploration of Women’s Experiences of Sexual Function after Female
Genital Cosmetic Surgery: A Phenomenological Descriptive Study
Authors: Nezhad Fatemeh Touhidi , Jalali Rostam , Karimi Fozieh , Menati Lida 
Published: March 2022

Background:
Female genital cosmetic surgery (FGCS) consists of a set of design approaches
to recover or strengthen the vulva. These cosmetic procedures are conducted to relieve
functionality problems such as vaginal looseness and sexual dissatisfaction. The external genital
tract is shaped cosmetically. Its results could be better judged through understanding the direct experience
of women who underwent surgery. Therefore, this study aimed to explain the experiences of
women regarding FGCS through phenomenological methods.

Methods:
In a phenomenological study with purposive sampling, semistructured in-depth interviews
were conducted on women who experienced FGCS. Then, the interviews were entered into the
Word software and were analyzed by the Colaizzi 7-step method. To ensure the correctness of the
data, the Guba’s criteria were used. Data were analyzed using MAXQDA software version 10.

Results:
The results showed that 15 women aged between 23-49 years old and with a mean age of 34 years old, mostly married and housewives as well as with diploma educational level participated in the study, three themes and eight subthemes were excluded from the analysis of participants’ interview. Participants presented their experiences on their sexual function after FGCS as sexual satisfaction, high-quality life, and willingness for surgery.

Conclusion:
The studies showed those women’s experiences with FGCS were positive, resulting in
sexual satisfaction and higher quality of life. Therefore, the assessment of women to perform cosmetic
surgery should be prioritized, and the women should be supported to make a decision regarding
such surgery.
Pioglitazone, Bladder Cancer, and the Presumption of Innocence
Authors: Papaetis Georgios S. 
Published: March 2022

Background:
Thiazolidinediones are potent exogenous agonists of PPAR-γ that augment
the effects of insulin to its cellular targets, mainly at the level of adipose tissue. Pioglitazone,
the main thiazolidinedione in clinical practice, has shown cardiovascular and renal benefits in patients
with type 2 diabetes, durable reduction of glycated hemoglobulin levels, important improvements
of several components of the metabolic syndrome, and beneficial effects of non-alcoholic
fatty liver disease.

Objective:
Despite all of its established advantages, the controversy for an increased risk of developing
bladder cancer, combined with the advent of newer drug classes that achieved major cardiorenal
effects, have significantly limited its use spreading a persistent shadow of doubt for its future
role.

Methods:
Pubmed, Google, and Scope databases have been thoroughly searched, and relevant
studies were selected.

Results:
This paper thoroughly explores both in vitro and in vivo (animal models and humans)
studies that investigated the possible association of pioglitazone with bladder cancer.

Conclusion:
Currently, the association of pioglitazone with bladder cancer cannot be based on solid
evidence. This evidence cannot justify its low clinical administration, especially in the present
era of individualised treatment strategies. Definite clarification of this issue is imperative and urgently
anticipated from future high quality and rigorous pharmacoepidemiologic research, keeping
in mind its unique mechanism of action and its significant pleiotropic effects.
NetDDS: A Real-time Interactive Platform Based on the Publish-subscribe
Mechanism
Authors: Zheng Pengyi , Rao Yuan 
Published: March 2022

Background:
With the continuous development of big data, artificial intelligence,
the Internet of things, and blockchain technology, distributed interactive systems
have gradually become the mainstream of computing technologies and applications with
the help of real-time interaction platforms. The real-time interaction platforms provide a
data communication bridge for distributed applications, not only meeting the timeliness
and correctness of data interaction but also providing a unified interface for application
development. It realizes the plug and play of different functional modules in the distributed
system and improves development efficiency for system developers.

Objective:
According to the characteristics of the large scale, wide range, and complex
running environment of distributed interaction systems, this paper analyzes the function
and performance requirements of the distributed interactive platform and designs and implements
a real-time interactive platform named NetDDS based on a publish-subscribe
mechanism.

Methods:
Concerning the idea of a layered design, the real-time interactive platform is
constructed from three aspects: distribution service layer, QoS guarantee layer, and data
transmission layer. The platform provides a data synchronization function based on publish/
subscribe mechanism, a configurable QoS guarantee mechanism, and a multi-protocol
data transmission mechanism based on Ethernet, ensuring the real-time correctness and
reliability of data transmission.

Results:
Experimental results show that the interactive platform exhibits less delay in message
update.

Conclusion:
This paper proposes a hierarchical architecture, designs and implements a
real-time interaction platform based on publish/subscribe mechanism, and provides a topic-
based publish/subscribe model to the application layer. The publisher and subscriber
directly publish and subscribe with data as the center to meet the real-time requirements of
distributed real-time applications.
Predicting Long-term Survival After Allogeneic Hematopoietic Cell Transplantation in Patients With Hematologic Malignancies: Machine Learning–Based Model Development and Validation
Authors: Choi Eun-Ji , Jun Tae Joon , Park Han-Seung , Lee Jung-Hee , Lee Kyoo-Hyung , Kim Young-Hak , Lee Young-Shin , Kang Young-Ah , Jeon Mijin , Kang Hyeran , Woo Jimin , Lee Je-Hwan 
Published: March 2022

Background
Scoring systems developed for predicting survival after allogeneic hematopoietic cell transplantation (HCT) show suboptimal prediction power, and various factors affect posttransplantation outcomes.

Objective
A prediction model using a machine learning–based algorithm can be an alternative for concurrently applying multiple variables and can reduce potential biases. In this regard, the aim of this study is to establish and validate a machine learning–based predictive model for survival after allogeneic HCT in patients with hematologic malignancies.

Methods
Data from 1470 patients with hematologic malignancies who underwent allogeneic HCT between December 1993 and June 2020 at Asan Medical Center, Seoul, South Korea, were retrospectively analyzed. Using the gradient boosting machine algorithm, we evaluated a model predicting the 5-year posttransplantation survival through 10-fold cross-validation.

Results
The prediction model showed good performance with a mean area under the receiver operating characteristic curve of 0.788 (SD 0.03). Furthermore, we developed a risk score predicting probabilities of posttransplantation survival in 294 randomly selected patients, and an agreement between the estimated predicted and observed risks of overall death, nonrelapse mortality, and relapse incidence was observed according to the risk score. Additionally, the calculated score demonstrated the possibility of predicting survival according to the different transplantation-related factors, with the visualization of the importance of each variable.

Conclusions
We developed a machine learning–based model for predicting long-term survival after allogeneic HCT in patients with hematologic malignancies. Our model provides a method for making decisions regarding patient and donor candidates or selecting transplantation-related resources, such as conditioning regimens.
Nurses’ Perspectives on an Electronic Medication Administration Record in Home Health Care: Qualitative Interview Study
Authors: Karnehed Sara , Erlandsson Lena-Karin , Norell Pejner Margaretha 
Published: March 2022

Background
eHealth is considered by policy makers as a prerequisite for meeting the demands of health care from the growing proportion of older people worldwide. The expectation about what the efficiency of eHealth can bring is particularly high in the municipal home health care sector, which is facing pressure regarding resources because of, for example, earlier discharges from hospitals and a growing number of patients receiving medications and treatments at home. Common eHealth services in home health care are electronic medication administration records (eMARs) that aim to communicate delegated tasks between professionals. However, there is an extensive gap in the research on how technology affects and is experienced by home health care professionals.

Objective
The objective of this paper is to shed light on how home care nurses experience eMARs in a Swedish municipality.

Methods
This qualitative interview study was conducted among home health care nurses using eMARs to facilitate communication and signing of delegated nursing tasks. The analysis of the interviews was performed using constructivist grounded theory, according to Charmaz.

Results
Of the 19 day-employed nurses in the municipality where an eMAR was used, 16 (84%) nurses participated in the study. The following two categories were identified from the focus group interviews: nurses become monitors and slip away from the point of care. The nurses experienced that they became monitors of health care through the increased transparency provided by the eMAR and the measurands they also applied, focusing on the quantitative aspects of the delegated nursing tasks rather than the qualitative aspects. The nurses experienced that their monitoring changed the power relations between the professions, reinforcing the nurses’ superior position. The experience of the eMAR was regarded as transitioning the nurses’ professional role—away from the point of care and toward more administration—and further strengthened the way of managing work through delegation to health care assistants.

Conclusions
Previous analyses of eHealth services in health care showed that implementation is a complex process that changes health care organizations and the work of health care professionals in both intended and unintended ways. This study adds to the literature by examining how users of a specific eHealth service experience its impacts on their daily work. The results indicate that the inscribed functions in an eHealth service may affect the values and priorities where the service is in use. This presents an opportunity for future research and for health care organizations to assess the impacts of specific eHealth services on health care professionals’ work and to further examine the effects of inscribed functions in relation to how they may affect actions and priorities at individual and organizational levels.
The Effect of Fear of Infection and Sufficient Vaccine Reservation Information on Rapid COVID-19 Vaccination in Japan: Evidence From a Retrospective Twitter Analysis (Preprint)
Authors: Niu Qian , Liu Junyu , Kato Masaya , Nagai-Tanima Momoko , Aoyama Tomoki 
Published: March 2022

BACKGROUND

The global public health and socioeconomic impacts of the COVID-19 pandemic have been substantial, rendering herd immunity by COVID-19 vaccination an important factor for protecting people and retrieving the economy. Among all the countries, Japan became one of the countries with the highest COVID-19 vaccination rates in several months, although vaccine confidence in Japan is the lowest worldwide.

OBJECTIVE

We attempted to find the reasons for rapid COVID-19 vaccination in Japan given its lowest vaccine confidence levels worldwide, through Twitter analysis.

METHODS

We downloaded COVID-19–related Japanese tweets from a large-scale public COVID-19 Twitter chatter data set within the timeline of February 1 and September 30, 2021. The daily number of vaccination cases was collected from the official website of the Prime Minister’s Office of Japan. After preprocessing, we applied unigram and bigram token analysis and then calculated the cross-correlation and Pearson correlation coefficient (<i>r</i>) between the term frequency and daily vaccination cases. We then identified vaccine sentiments and emotions of tweets and used the topic modeling to look deeper into the dominant emotions.

RESULTS

We selected 190,697 vaccine-related tweets after filtering. Through n-gram token analysis, we discovered the top unigrams and bigrams over the whole period. In all the combinations of the top 6 unigrams, tweets with both keywords “reserve” and “venue” showed the largest correlation with daily vaccination cases (<i>r</i>=0.912; <i>P</i>&lt;.001). On sentiment analysis, negative sentiment overwhelmed positive sentiment, and fear was the dominant emotion across the period. For the latent Dirichlet allocation model on tweets with fear emotion, the two topics were identified as “infect” and “vaccine confidence.” The expectation of the number of tweets generated from topic “infect” was larger than that generated from topic “vaccine confidence.”

CONCLUSIONS

Our work indicates that awareness of the danger of COVID-19 might increase the willingness to get vaccinated. With a sufficient vaccine supply, effective delivery of vaccine reservation information may be an important factor for people to get vaccinated. We did not find evidence for increased vaccine confidence in Japan during the period of our study. We recommend policy makers to share accurate and prompt information about the infectious diseases and vaccination and to make efforts on smoother delivery of vaccine reservation information.
ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI KEPUTUSAN PEMBELIAN ONLINE MAHASISWA DI KOTA MEDAN PADA APLIKASI SHOPEE
Authors: Adhitya Wisnu Rayhan 
Published: March 2022

<p><em>Permasalahan dalam penelitian ini adalah perubahan paradigma para Mahasiswa di Kota Medan dalam sikap berbelanja yang duulunya terbiasa secara konvensional atau tradisional sekarang sudah cenderung mengarah modern dengan menggunakan teknologi yang berkembang pesat saat ini. Perubahan paradigma belanja ini biasa disebut dengan online shopping yaitu melakukan kegiatan jual beli dengan menggunakan infrastruktur hardware, software dan jaringan internet dalam prosesnya. </em><em>Tujuan penelitian ini </em><em>adalah untuk mengetahui secara simultan dan parsial variabel kualitas produk, harga produk, kemudahan berbelanja dan ulasan produk terhadap </em><em>keputusan pembelian online Mahasiswa di Kota Medan. Metode analisis data yang digunakan adalah deskriptif kuantitatif dengan menggunakan analisis regresi linier berganda. Variabel kualitas produk (X1) secara parsial berpengaruh positif dan signifikan terhadap keputusan pembelian (Y), harga produk (X2) secara parsial berpengaruh positif dan signifikan terhadap keputusan pembelian (Y), kemudahan berbelanja (X3) secara parsial berpengaruh positif dan signifikan terhadap keputusan pembelian (Y), ulasan produk (X4) secara parsial berpengaruh positif dan signifikan terhadap keputusan pembelian (Y), kualitas produk (X1), harga produk (X2), kemudahan berbelanja (X3), dan ulasan produk (X4) secara simultan berpengaruh positif dan signifikan terhadap keputusan pembelian (Y) online mahasiswa di kota Medan pada aplikasi Shopee.</em></p>
Analisis Perhitungan Tax Shield Approach Dalam Mendeterminasikan Arus Kas Operasional
Authors: Lubis Nurul Izzah , Mawarni Ade 
Published: March 2022

<em>Tax Shield Approach merupakan salah satu pendekatan untuk menghitung dan menganalisis Arus Kas Operasional suatu perusahaan. Penelitian ini bertujuan untuk menghitung dan menganalisis Tax Shield Approach dalam mendeterminasikan Arus Kas Operasional pada 9 perusahaan sub sector Konstruksi dan Bangunan periode 2017-2019 dengan jenis penelitian deskriptif sebagai analisis keberlanjutan. Tax Shield approach menggunakan indikator EBIT, Tarif Pajak, dan Depresiasi sebagai penyatu rumus. Adapun pendekatan ini dapat digunakan untuk dua manfaat yaitu manajemen perusahaan dan manajemen pajak. Kesimpulan yang didapatkan dari penelitian ini adalah pada tahun 2018, EBIT memiliki pertumbuhan sebesar -13% dari tahun 2017 dan menurun secara signifikan menjadi -73% di tahun 2019, pertumbuhan depresiasi di tahun 2018 sebesar 29% dari tahun sebelumnya dan menjadi 21% di tahun 2019, AKO/OCF memiliki pertumbuhan sebesar -7% di tahun 2018 dan menurun menjadi -55% di tahun 2019. Dari segi manajemen perusahaan, AKO/OCF yang menurun menjadi masalah bagi nilai perusahaan dan harus ditingkatkan untuk menjaga kepercayaan public maupun investor/kreditur. Dari segi manajemen perpajakan, AKO/OCF yang menurun menandakan Tax Shield atau perencanaan pajak yang baik karena dapat menghindari pajak untuk mengecilkan pengeluaran beban pajak penghasilan tanpa melanggar UU perpajakan.</em>
SEJARAH PERANG AFGHANISTAN DARI DULU HINGGA KINI
Authors: Azria Kaiva , Ramayani Erma 
Published: March 2022

<p>Perang merupakan cara yang <em>hard power</em> dalam ilmu hubungan internasional, dimana pada tahun 2001 saat terjadi serangan terhadap negara <em>super power </em>Amerika Serikat yang menyerang <em>World Trade Center</em> (WTC) bulan September dianggap merupakan tindakan terorisme, hingga pernyataan perang Amerika Serikat terhadap Afghanistan yang dianggap melindungi Al-Qaeda yaitu kelompok terorisme. Taliban yaitu sebuah kelompok atas kepolitikan sekaligus agama yang kontradiktif memiliki pengaruh dalam memberikan kebijakan kepada Afghanistan dan menggunakan kekuasaannya dalam melindungi Al-Qaeda. Konflik di kawasan Timur Tengah ini terus berlangsung hingga dengan genjatan senjata hingga dua darsawarsa, konflik ini telah melibatkan aksi militer Amerika Serikat tadinya ingin menarik pasukan militer, namun Taliban kembali memberontak ingin bergerak menguasai Afghanistan hingga pemberontakan terjadi lagi sampai saat ini. </p>
Seismic Characterization of Internal Salt Cycles: A Case Study in the Santos Basin, Brazil
Authors: Pontes Rodrigo Leandro Bastos , Maul Alexandre Rodrigo , Silva Cleverson Guizan 
Published: March 2022

ABSTRACT. The exploration of pre-salt reservoirs in the Santos Basin, Brazilian Offshore, requires innovative approaches to improve the quality of reservoir seismic images below large salt bodies, helping to enhance the depth predictions and to improve security for drilling operations. Salt is a general term for evaporites that may present an enormous complexity. Outcrop and experimental data show that evaporites precipitate in a controlled and defined order, respecting increasing brine salinity. When analyzing the Ariri Formation (salt section) in the Santos Basin we recognize several evaporation salt cycles, indicating climatic and tectonic controls. In this work, we identify the salt cycles in wells and integrate those with the seismic data as low-frequency events to create detailed velocity models. Our results confirm that the incorporation of the salt cycles reduces the average mismatch of depth positioning, consequently, providing reliable volumetric estimations.Keywords: evaporitic section, salt cycles, seismic imaging, seismic inversion, velocity model. Caracterização Sísmica de Ciclos Evaporíticos: Estudo de Caso na Bacia de Santos, BrasilRESUMO. A exploração de reservatórios do pré-sal na Bacia de Santos, Offshore Brasileiro, requer abordagens inovadoras para melhorar a qualidade das imagens sísmicas dos reservatórios abaixo de grandes corpos de sal, ajudando a tornar mais as previsões de profundidade mais precisas e a aumentar a segurança das operações de perfuração. Sal é um termo geral para evaporitos que podem apresentar uma enorme complexidade. Dados experimentais e de afloramentos mostram que os evaporitos precipitam em ordem bem controlada e definida, respeitando o aumento da salinidade da salmoura. Ao analisar a Formação Ariri (seção de sal) na Bacia de Santos reconhecemos vários ciclos de evaporação do sal, indicando controles climáticos e tectônicos. Neste trabalho, identificamos os ciclos de sal em poços e os integramos aos dados sísmicos como eventos de baixa frequência para criar modelos de velocidade detalhados. Nossos resultados confirmam que a incorporação dos ciclos de sal reduz o erro médio do posicionamento em profundidade e, consequentemente, fornecendo estimativas volumétricas mais confiáveis.Palavras-chave: seção evaporítica, ciclos salinos, imageamento sísmico, inversão sísmica, modelo de velocidade.