bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sokak Sütlerinde Gizli Tehlike: Antibiyotik Kalıntısı
Authors: Mortaş Hande , Navruz Varlı Semra , Bilici Saniye 
Published: May 2022

Amaç: Süt hayvanlarının sağlığını korumak amacıyla kullanılan antibiyotiklerin sütlerde bıraktığı antibiyotik kalıntıları insan sağlığını önemli derecede tehdit etmektedir. Ayrıca sütteki antibiyotik kalıntılarının ısıl işlemlerle nötralize edilemediği de bildirilmektedir. Bu nedenlerle denetimsiz satılan çiğ sütlerin antibiyotik kalıntısı açısından değerlendirilmesi son derece önemlidir. Bu çalışmada, Ankara ilinde sokakta satılan çiğ sütlerin içerdiği antibiyotik kalıntılarının araştırılması amaçlanmıştır.
Gereç ve Yöntem: Bu çalışmanın örneklemini oluşturan sokak sütlerinin Ankara ilini temsil etmesi için Ankara’nın en yoğun nüfusa sahip 9 ilçesinden nüfus yoğunluklarına göre belirlenen sayılarda toplam 60 adet sokak sütü numunesi araştırmacılar tarafından toplanmıştır. Alınan sokak sütleri Idexx Snap ST Plus DUO kullanılarak antibiyotik kalıntıları açısından araştırılmıştır.
Bulgular: Çalışmada alınan sokak sütü numunelerinin toplam %30’unda antibiyotik kalıntı miktarlarının yönetmelikte belirlenen limitleri aştığı tespit edilmiştir. Bunlardan %28.3’ünün (n=17) beta-laktam kalıntı içeriğinin, %1.7’sinin (n=1) ise tetrasiklin kalıntı içeriğinin mevzuatta belirlenen limitlerin üzerinde olduğu saptanmıştır.
Sonuç: Ankara’da satışa sunulan sokak sütlerinde tespit edilen antibiyotik kalıntılarının mevzuata uygun olmaması, antibiyotik kalıntı maruziyetinin neden olabileceği sağlık risklerinin yanı sıra antibiyotik direnci gelişimine de katkı sağlayabileceği için endişe vericidir. Son yıllarda doğal olduğu düşüncesi ile artan çiğ süt tüketim eğilimi de göz önünde bulundurulduğunda denetimsiz olarak satılan sokak sütlerinin satışı ile ilgili yaptırım ve denetimlerin arttırılması ve bu konuda tüketicilerin bilinçlendirilmesi önemlidir.
Vision of Mountain Warfare Development in the Slovenian Armed Forces
Authors: Polanec Bernard 
Published: May 2022

Abstract
In the article, we will first introduce mountain warfare and explain the individual concepts related to mountain warfare. Further on, we will focus on contemporary warfare, including mountain warfare, and the importance of mountain warfare in the future. We will conclude the first part of the article by presenting the current situation regarding mountain units in the Slovenian Armed Forces.
In the second part, we will present the challenges faced by the Slovenian Armed Forces in the field of mountain warfare, and, finally, present our vision for the development of the Slovenian Armed Forces and mountain warfare, including some solutions in response to the challenges presented in the article.
Improving the Stability of a Hemipelvic Prosthesis Based on Bone Mineral Density Screw Channel and Prosthesis Optimization Design
Authors: Zhou Rongqi , Xue Haowen , Wang Jincheng , Wang Xiaonan , Wang Yanbing , Zhang Aobo , Zhang Jiaxin , Han Qing , Zhao Xin 
Published: May 2022

In pelvic reconstruction surgery, the hemipelvic prosthesis can cause significant changes in stress distribution due to its high stiffness, and its solid structure is not suitable for osseointegration. The purpose of this study was to identify a novel bone mineral density screw channel and design the structure of the prosthesis so as to improve the distribution of stress, promote bone growth, and enhance the biomechanical properties of the prosthesis. The mechanical characteristics of bone mineral density screw and traditional screw were compared by finite element analysis method, and redesigned by topology optimization. The direction of the newly proposed screw channel was the posterolateral entrance of the auricular surface, ending at the contralateral sacral cape. Compared to the original group, the maximum stress of the optimized prosthesis was decreased by 24.39%, the maximum stress of the sacrum in the optimized group was decreased by 27.23%, and the average strain energy density of the sacrum in the optimized group was increased by 8.43%. On the surface of screw and connecting plate, the area with micromotion more than 28 μm is reduced by 12.17%. On the screw surface, the area with micromotion more than 28 μm is reduced by 22.9%. The newly determined screw channel and optimized prosthesis design can effectively improve the biomechanical properties of a prosthesis and the microenvironment of osseointegration. This method can provide a reference for the fixation of prostheses in clinical pelvic reconstruction.
Dynamic Epicardial Contribution to Cardiac Interstitial c-Kit and Sca1 Cellular Fractions
Authors: Pogontke C. , Guadix J. A. , Sánchez-Tévar A. M. , Muñoz-Chápuli R. , Ruiz-Villalba A. , Pérez-Pomares J. M. 
Published: May 2022

Background: The cardiac interstitial cellular fraction is composed of multiple cell types. Some of these cells are known to express some well-known stem cell markers such as c-Kit and Sca1, but they are no longer accepted to be true cardiac stem cells. Although their existence in the cardiac interstitium has not been disputed, their dynamic throughout development, specific embryonic origin, and potential heterogeneity remain unknown. In this study, we hypothesized that both c-KitPOS and Sca1POS cardiac interstitial cell (CIC) subpopulations are related to the Wilms’ tumor 1 (Wt1) epicardial lineage.Methods: In this study, we have used genetic cell lineage tracing methods, immunohistochemistry, and FACS techniques to characterize cardiac c-KitPOS and Sca1POS cells.Results: Our data show that approximately 50% of cardiac c-KitPOS cells are derived from the Wt1-lineage at E15.5. This subpopulation decreased along with embryonic development, disappearing from P7 onwards. We found that a large proportion of cardiac c-KitPOS cells express specific markers strongly suggesting they are blood-borne cells. On the contrary, the percentage of Sca1POS cells within the Wt1-lineage increases postnatally. In accordance with these findings, 90% of adult epicardial-derived endothelial cells and 60% of mEFSK4POS cardiac fibroblasts expressed Sca1.Conclusion: Our study revealed a minor contribution of the Wt1-epicardial lineage to c-KitPOS CIC from embryonic stages to adulthood. Remarkably, a major part of the adult epicardial-derived cell fraction is enriched in Sca1, suggesting that this subpopulation of CICs is heterogeneous from their embryonic origin. The study of this heterogeneity can be instrumental to the development of diagnostic and prognostic tests for the evaluation of cardiac homeostasis and cardiac interstitium response to pathologic stimuli.
Effect of Sacubitril-Valsartan on Restoration and Maintenance of Sinus Rhythm in Patients With Persistent Atrial Fibrillation
Authors: Chen Qingsong , Chen Yunlin , Qin Fang , Du Huaan , Gan Chunxia , Zhou Bei , Wang Na , Xiao Mingyang , Ou Zhenhong , Zhao Wei , Cui Ben , Liu Zengzhang , Yin Yuehui 
Published: May 2022

BackgroundExisting studies have shown that sacubitril-valsartan ameliorated atrial remodeling in atrial fibrillation (AF) and favored maintenance of sinus rhythm in patients with AF and heart failure. However, the effect of sacubitril-valsartan in patients with persistent AF is yet unknown. We aimed to evaluate the effect of sacubitril-valsartan on restoration and maintenance of sinus rhythm in patients with persistent AF who underwent electrical cardioversion (ECV).MethodConsecutive patients with persistent AF who underwent ECV between 1 January 2016 and 30 September 2020 were investigated in this retrospective cohort study. All eligible patients were categorized into sacubitril-valsartan users and sacubitril-valsartan non-users based on whether they received treatment with sacubitril-valsartan or not. The endpoint was ineffictive ECV, defined as the composite of failure to terminate AF or any recurrence of AF during 30 days follow-up.ResultsA total of 76 patients were enrolled in this study, including 28 sacubitril-valsartan users and 48 non-users. Within a follow-up of 30 days after ECV, the endpoint had occurred in 7 (25%) of 28 sacubitril-valsartan users and 25 (52%) of 48 non-users. Significantly lower rate of ineffictive ECV in sacubitril-valsartan users compared with non-users was shown in Kaplan-Meier survival curves (P = 0.02; Log-rank test). Multivariate Cox regression analysis indicated that sacubitril-valsartan use (hazard ratio [HR], 0.35; 95% confidence interval [CI], 0.14–0.91), amiodarone use (HR, 0.32; 95% CI, 0.13–0.78), left atrial diameter ≤ 39 mm (HR, 0.21; 95% CI, 0.06–0.71) were independently associated with a decreased rate of ineffective electrical cardioversion.ConclusionUse of sacubitril-valsartan is associated with a significantly decreased risk of ineffective ECV compared with non-users in patients with persistent AF.
Comparison of Underground Coal Mining Methods Based on Life Cycle Assessment
Authors: Wu Haoyuan , Yin Zhiqiang , Zhang Yuchen , Qi Chao , Liu Xian , Wang Jianen 
Published: May 2022

Coal is China’s main source of energy, and its production causes a certain degree of air, water, and land resource pollution. Therefore, it is necessary to compare environmental pollution caused by different mining methods. When mining underground coal resources, different mining methods have different levels of environmental impacts. To identify the mining method with the least environmental impact, different mining methods were evaluated by means of a life cycle assessment using Simapro 9.0.0 software. The Ecoinvent v3 database was used to provide background data, and the results were calculated using the Eco-indicator99 method. The findings show that for a 20 m coal column, respiratory inorganics were identified as the dominant impact category based on normalized results, followed by fossil fuels, carcinogens, and climate change. The contribution analysis indicated that electricity and coal mining are fundamental in contributing to these significant impact categories and should be of particular concern. According to the sensitivity analysis, reducing mining activities and increasing extraction efficiency and use are the primary responses to addressing environmental pollution, followed by reducing environmental pollution caused by electricity and steel production. In addition, a summary comparison of the single scores of different mining methods suggests that the environmental burden of pillarless mining is the lowest, and as the width of the coal pillar gradually increases, its single score shows a trend of increasing and then decreasing. Therefore, the single score of non-pillar mining is the lowest compared to that of other mining methods and can be the optimal mining method. This study can provide a scientific basis for the selection of green mining and underground coal resource mining methods.
Geospatial Environmental Influence on Forest Carbon Sequestration Potential of Tropical Forest Growth in Hainan Island, China
Authors: Lin Meizhi , Song Yanni , Lu Di , Qiu Zixuan 
Published: May 2022

Tropical forests, although covering only 7% of the world’s land area, have great forest carbon sequestration capacity, accounting for 20% of the world’s forest carbon sink. However, the growth dynamics and forest carbon sink potential of tropical forests remain unclear. Hainan Island is going to be China’s forest carbon trading center. Therefore, accurately assessing the future forest carbon sink potential of Hainan Island’s tropical forest is crucial. In this study, 393 forest permanent sample plots in Hainan Island in 2003, 2008, 2013, and 2018 were selected as the research objects. The dynamic model of tropical forest growth with the geospatial environmental indicators was established based on the measured and most accurate annual diameter at breast height (DBH) growth factors. The DBH growth prediction’s bias ranged from 0.46 to 0.07 cm, RMSE ranged from 1.50 to 5.29 cm, bias% ranged from -2.96 to 0.55%, and RRMSE ranged from 12.18 to 34.30%. In addition, the geospatial environmental indicators of forest growth provide scientific guidance for future ecological protection and land evolution of Hainan Island. Based on DBH–tree height–volume, volume–biomass, and biomass–forest carbon storage relationships, forest carbon sequestration potential could be accurately evaluated by DBH growth. The results show that within the next 30 years, the forest carbon sequestration in Hainan Island will account for 1.8% of the total forest carbon sequestration in China, while the forest area will only account for 0.88% of the total forest area in China. It is roughly estimated that in the next 30 years, the total carbon sink of the tropical forest in Hainan Island will be 83.59 TgC. This study further proves that the annual increase in DBH can accurately assess the forest carbon sink potential of the forest. The forest carbon sink prediction based on the annual increase in DBH can provide data support and theoretical basis for forest carbon sink trading between forest farms and enterprises.
Predicting the Settlement of Mine Waste Dump Using Multi-Source Remote Sensing and a Secondary Consolidation Model
Authors: Tabish Reza , Yang Zefa , Wu Lixin , Xu Zhihua , Cao Zhanpeng , Zheng Kaihui , Zhang Yanqing 
Published: May 2022

The settlement (or subsidence) of mine waste dump is likely to cause landslides, thereby imposing threats on the safety of human beings and other local properties. Hence, it is essential to accurately predict the settlement for the early-warning of settlement-induced geohazards. Traditional mechanical methods require in situ mechanical parameters and the geological structure of mine waste dump, narrowing their practical applications. In this paper, we proposed a remote sensing-based method for predicting the settlement of mine waste dump without the requirement for field observations. Firstly, the historical settlement and thickness of mine waste dump were measured by space-borne interferometric synthetic aperture radar (InSAR) and photogrammetry techniques, respectively. Secondly, the temporal evolution of the settlement of mine waste dump was described by a secondary consolidation model. Thirdly, the parameters of the secondary consolidation model were inverted based on the InSAR-measured historical settlements and photogrammetry-estimated thickness. Finally, the evolution trend of the settlement of the mine waste dump was predicted with the secondary consolidation model and its inverted parameters. The proposed method was tested over a mine waste dump in Weijiamao open-pit mine, China. The result shows that the root mean square error of the predicted time-series settlement is about 0.8 cm. The presented method will be beneficial to the assessment and early-warning of the settlement-related geohazards of mine waste dumps over a large area.
The Inflamm-Aging Model Identifies Key Risk Factors in Atherosclerosis
Authors: He Yudan , Chen Yao , Yao Lilin , Wang Junyi , Sha Xianzheng , Wang Yin 
Published: May 2022

Background: Atherosclerosis, one of the main threats to human life and health, is driven by abnormal inflammation (i.e., chronic inflammation or oxidative stress) during accelerated aging. Many studies have shown that inflamm-aging exerts a significant impact on the occurrence of atherosclerosis, particularly by inducing an immune homeostasis imbalance. However, the potential mechanism by which inflamm-aging induces atherosclerosis needs to be studied more thoroughly, and there is currently a lack of powerful prediction models.Methods: First, an improved inflamm-aging prediction model was constructed by integrating aging, inflammation, and disease markers with the help of machine learning methods; then, inflamm-aging scores were calculated. In addition, the causal relationship between aging and disease was identified using Mendelian randomization. A series of risk factors were also identified by causal analysis, sensitivity analysis, and network analysis.Results: Our results revealed an accelerated inflamm-aging pattern in atherosclerosis and suggested a causal relationship between inflamm-aging and atherosclerosis. Mechanisms involving inflammation, nutritional balance, vascular homeostasis, and oxidative stress were found to be driving factors of atherosclerosis in the context of inflamm-aging.Conclusion: In summary, we developed a model integrating crucial risk factors in inflamm-aging and atherosclerosis. Our computation pipeline could be used to explore potential mechanisms of related diseases.
Electrochemical Studies of LbL Films With Dawson Type Heteropolyanion Glassy Carbon Electrode Sensor Modified for Methyl Parathion Detection
Authors: Ditta Nafeesa Allah , Yaqub Mustansara , Nadeem Sohail , Jamil Sundas , Hassan Sadaf Ul , Iqbal Shahid , Javed Mohsin , Elkaeed Eslam B. , Alshammari Fwzah H. , Alwadai Norah , Alzhrani Rami M. , Awwad Nasser S. , Ibrahium Hala A. 
Published: May 2022

Rapid methyl parathion detection was measured using a fabricated glassy carbon electrode (GCE) sensor designed using the layer-by-layer (LBL) method. Multilayer assemblies were developed on the glassy carbon electrode by alternating depositions of anions and cations in which a Dawson Type Polyoxometalate β-K6[(P2W18O62·H2O)]·14H2O (∼P2W18 POM) and polyethyleneimine (PEI) stabilized silver nanoparticles (∼PEI-AgNPs) acted as anions and cations, respectively. The redox behavior of P2W18 POM within LBL assembly was carried out via cyclic voltammetry. This LBL assembly was thoroughly characterized by UV-Visible, FT-IR, XRD, AFM, and SEM techniques. The fabricate GCE sensor was investigated for the electrocatalytic activity to detect methyl parathion. The results clearly showed that the fabricated GCE sensor was successfully synthesized. More interestingly, the current response for detecting methyl parathion was found to be less than 1 ppm, proving that this fabricated GCE sensor may exhibit potential applications in the detection of targeted pesticide.
Efficacy of the Integrative Acupuncture and Moxibustion Treatment in Patients With Major Depressive Disorder: The Study Protocol for a Multicenter, Single-Blinded, Randomized Trial in China
Authors: Zhang Yuan , Liu Yamin , Ning Baile , Yan Luda , Wu Lihua , Zhang Delong , Li Changhong , Ouyang Wenwei , Su Shengyong , Jiang Shuo , Zhang Guangcai , Xu Junfeng , Wang Zhen , Zheng Zhong , Zheng Dong , Chen Shan , Sun Lu , Fu Wenbin 
Published: May 2022

IntroductionAntidepressants are the front-line treatments for major depressive disorder (MDD), but remain unsatisfactory in outcome. An increasing number of patients are interested in acupuncture and moxibustion treatment as complementary therapies. This study aims to evaluate the efficacy and safety of integrative acupuncture and moxibustion (iAM) treatment in patients with MDD.Methods and AnalysisThis multicenter, single-blind, 2 × 2 factorial randomized trial will enroll 592 patients with MDD of moderate severity from nine hospitals. All patients will be randomized, in a ratio of 2:2:2:1, through a computerized central randomization system, into four groups (the combined, iAM-only, sertraline-only, and placebo groups). Participants will undergo a 12-week intervention with either 50 mg of sertraline or a placebo once a day and active/sham iAM treatment three times per week. The primary outcome is depression severity, assessed using the Hamilton Depression Scale-17. The secondary outcomes include self-rated depression severity, anxiety, and sleep quality. The primary and secondary outcomes will be measured at weeks 0, 4, 8, 12, and the 8th week posttreatment. Safety will be evaluated through liver and kidney function tests conducted before and after treatment and through monitoring of daily adverse events. An intent-to-treat principle will be followed for the outcome analyses.ConclusionThis trial will provide sufficient evidence to ascertain whether iAM is effective and safe for treating MDD and provides a suitable combination strategy for treating MDD.Clinical Trial Registration[www.chictr.org.cn], identifier [ChiCTR2100042841].
Neurotrophic Keratopathy in Systemic Diseases: A Case Series on Patients Treated With rh-NGF
Authors: Meduri Alessandro , Oliverio Giovanni William , Valastro Antonio , Azzaro Claudia , Camellin Umberto , Franchina Francesco , Inferrera Leandro , Roszkowska Anna , Aragona Pasquale 
Published: May 2022

PurposeTo evaluate the prevalence, clinical ocular presentation and corneal healing in moderate and severe neurotrophic keratopathy (NK) caused by systemic diseases and treated with rh-NGF.SettingDepartment of Biomedical and Dental Sciences and Morphofunctional Imaging, Ophthalmology Clinic, University of Messina, Italy.DesignRetrospective observational study of case series.Materials and MethodsIn this retrospective observational study 11 patients (five female and six males) aged from 24 to 88 years (55.4 ± 21.3 years) with moderate and severe NK caused by systemic diseases were enrolled. The VAS questionnaire was dispensed. The ocular examination comprised slit lamp evaluation, ocular surface assessment with Keratograph 5M (Oculus, Germany), corneal sensitivity with Cochet-Bonnet esthesiometer (Lunneaux, France) and corneal thickness measurement with AC-OCT (DRI, Triton, Topcon, Japan). The underlying systemic causes of NK were determined.ResultsThe main cause of NK was post-neuroma surgery (36%), followed by diabetes (18%). The remaining causes were rheumatoid arthritis (9%), post-traumatic (9%), post-surgery (9%), atopia (9%), Graves' disease (9%). Seven eyes presented severe grade of NK with corneal ulcer and in four a moderate grade was registered. The rh-NGF (Cenegermin) was administered with a standard protocol one drop six times daily for 8 weeks. The complete healing of all corneal defects was registered at the end of the treatment.ConclusionsThe post-neuroma surgery was the most common cause of NK and severe grade was clinically more represented. The rh-NGF proved effective to promote corneal recovery with all defects healed after the treatment.
Salubrinal Protects Against Cisplatin-Induced Cochlear Hair Cell Endoplasmic Reticulum Stress by Regulating Eukaryotic Translation Initiation Factor 2α Signalling
Authors: Lu Wen , Ni Kun , Li Zhuangzhuang , Xiao Lili , Li Yini , Jiang Yumeng , Zhang Jincheng , Shi Haibo 
Published: May 2022

ObjectiveCisplatin is a broad-spectrum anti-tumour drug commonly used in clinical practice. However, its ototoxicity greatly limits its clinical application, and no effective method is available to prevent this effect. Endoplasmic reticulum stress (ERS) is reportedly involved in cisplatin ototoxicity, but the exact mechanism remains unclear. Therefore, this study aimed to investigate the role of eukaryotic translation initiation factor 2α (eIF2α) signalling and its dephosphorylation inhibitor salubrinal in cisplatin ototoxicity.MethodsWe evaluated whether salubrinal could protect against cisplatin-induced damage in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and mouse cochlear explants. By knocking down eIF2α, we elucidated the vital role of eIF2α in cisplatin-induced damage in HEI-OC1 cells. Whole-mount immunofluorescent staining and confocal microscopy of mouse cochlear explants and HEI-OC1 cells were performed to analyse cisplatin-induced damage in cochlear hair cells and the auditory cell line.ResultsData suggested salubrinal attenuated cisplatin-induced hair cell injury by inhibiting apoptosis. In addition, salubrinal significantly reduced ERS levels in hair cells via eIF2α signalling, while eIF2α knockdown inhibited the protective effect of salubrinal.SignificanceSalubrinal and eIF2α signalling play a role in protecting against cisplatin-induced ototoxicity, and pharmacological inhibition of eIF2α-mediated ERS is a potential treatment for cisplatin-induced damage in the cochlea and HEI-OC1 cells.