bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired detection of omicron by SARS-CoV-2 rapid antigen tests
Authors: Osterman Andreas , Badell Irina , Basara Elif , Stern Marcel , Kriesel Fabian , Eletreby Marwa , Öztan Gamze Naz , Huber Melanie , Autenrieth Hanna , Knabe Ricarda , Späth Patricia M. , Muenchhoff Maximilian , Graf Alexander , Krebs Stefan , Blum Helmut , Durner Jürgen , Czibere Ludwig , Dächert Christopher , Kaderali Lars , Baldauf Hanna-Mari , Keppler Oliver T. 
Published: February 2022

AbstractSince autumn 2020, rapid antigen tests (RATs) have been implemented in several countries as an important pillar of the national testing strategy to rapidly screen for infections on site during the SARS-CoV-2 pandemic. The current surge in infection rates around the globe is driven by the variant of concern (VoC) omicron (B.1.1.529). Here, we evaluated the performance of nine SARS-CoV-2 RATs in a single-centre laboratory study. We examined a total of 115 SARS-CoV-2 PCR-negative and 166 SARS-CoV-2 PCR-positive respiratory swab samples (101 omicron, 65 delta (B.1.617.2)) collected from October 2021 until January 2022 as well as cell culture-expanded clinical isolates of both VoCs. In an assessment of the analytical sensitivity in clinical specimen, the 50% limit of detection (LoD50) ranged from 1.77 × 106 to 7.03 × 107 RNA copies subjected to the RAT for omicron compared to 1.32 × 105 to 2.05 × 106 for delta. To score positive in these point-of-care tests, up to 10-fold (LoD50) or 101-fold (LoD95) higher virus loads were required for omicron- compared to delta-containing samples. The rates of true positive test results for omicron samples in the highest virus load category (Ct values < 25) ranged between 31.4 and 77.8%, while they dropped to 0–8.3% for samples with intermediate Ct values (25–30). Of note, testing of expanded virus stocks suggested a comparable RAT sensitivity of both VoCs, questioning the predictive value of this type of in vitro-studies for clinical performance. Given their importance for national test strategies in the current omicron wave, awareness must be increased for the reduced detection rate of omicron infections by RATs and a short list of suitable RATs that fulfill the minimal requirements of performance should be rapidly disclosed.
Auriculotemporal Frey syndrome not associated with surgery or diabetes: systematic review
Authors: Betti Céline , Milani Gregorio P. , Lava Sebastiano A. G. , Bianchetti Mario G. , Bronz Gabriel , Ramelli Gian P. , Goeggel Simonetti Barbara , Bergmann Marcel M. 
Published: February 2022

AbstractPatients who undergo salivary gland, neck, or facelift surgery or suffer from diabetes mellitus often develop Frey syndrome (also known as auriculotemporal syndrome or gustatory sweating). Frey syndrome has been occasionally reported to occur in subjects without history of surgery or diabetes but this variant of Frey syndrome has not been systematically investigated. We searched for original articles of Frey syndrome unrelated to surgery or diabetes without date and language restriction. Article selection and data extraction were performed in duplicate. Our systematic review included 76 reports describing 121 individual cases (67 males and 54 females) of Frey syndrome not associated with surgery or diabetes. The age at onset of symptoms was ≤ 18 years in 113 (93%) cases. The time to diagnosis was 12 months or more in 55 (45%) cases. On the other hand, an allergy evaluation was performed in half of the cases. A possible cause for Frey syndrome was detected in 85 (70%) cases, most frequently history of forceps birth (N = 63; 52%). The majority of the remaining 22 cases occurred after a blunt face trauma, following an auriculotemporal nerve neuritis or in association with a neurocutaneous syndrome. The cause underlying Frey syndrome was unknown in 36 cases. Conclusion: Frey syndrome not associated with surgery or diabetes almost exclusively affects subjects in pediatric age and is uncommon and underrecognized. Most cases occur after forceps birth. There is a need to expand awareness of this pseudo-allergic reaction among pediatricians and allergists.What is Known:• Pre-auricular reddening, sweating, and warmth in response to mastication or a salivary stimulus characterize Frey syndrome.• It usually occurs after salivary gland surgery and in diabetes.What is New:• In children, Frey syndrome is rare, and most cases occur after a forceps-assisted birth.• In childhood, this condition is often erroneously attributed to food allergy.
Anti-CD20–atezolizumab–polatuzumab vedotin in relapsed/refractory follicular and diffuse large B-cell lymphoma
Authors: Topp Max S. , Eradat Herbert , Florschütz Axel , Hochhaus Andreas , Wrobel Tomasz , Walewski Jan , Knopinska-Posluszny Wanda , Kanate Abraham S. , Lech-Maranda Ewa , Brunnberg Uta , Chitra Surya , Nielsen Tina G. , Sellam Gila , Shivhare Mahesh , Lossos Izidore S. 
Published: February 2022

Abstract
Purpose
New therapies are needed for relapsed/refractory (R/R) B-cell non-Hodgkin lymphoma. This phase 1b, open-label trial evaluated two anti-CD20-based triplet combinations.

Methods
Patients with R/R follicular lymphoma (FL; n = 13) were treated with obinutuzumab, atezolizumab, and polatuzumab vedotin (G-atezo-pola; 1.4 mg/kg/1.8 mg/kg) and patients with R/R diffuse large B-cell lymphoma (DLBCL; n = 23) received rituximab (R)-atezo-pola. The primary efficacy endpoint was complete response (CR) at end of induction (EOI) by PET-CT (investigator assessed; modified Lugano 2014 criteria). Safety endpoints were also assessed.

Results
13 FL patients were treated and evaluable for safety; 2/23 DLBCL patients did not receive treatment and were not included in the safety population. Median observation time was 23.3 and 5.7 months in the FL and DLBCL cohorts, respectively. At EOI, CR rates in FL patients treated with G-atezo-pola at pola doses of 1.4 mg/kg (N = 3) and 1.8 mg/kg (N = 7) were 33% and 14%, respectively. In DLBCL patients receiving R-atezo-pola, the CR rate at EOI was 13%. In the FL cohort, 62% of patients experienced a grade 3–5 adverse event (AE; including two deaths) and 31% developed a serious AE (SAE). In DLBCL patients, R-atezo-pola was associated with a lower incidence of grade 3–5 AEs (24%; one death) and SAEs (10%). In both cohorts, the most common grade 3–5 AEs were hematologic toxicities.

Conclusion
Based on these safety issues, considered as related specifically to G-atezo-pola, and limited efficacy, no further development of either combination is planned.

Trial registration
NCT02729896; Date of registration: April 6, 2016.
D,L-Methadone enhances the cytotoxic activity of standard chemotherapeutic agents on pediatric rhabdomyosarcoma
Authors: Urla Cristian , Corteletti Irene , Raible Ann-Sophie , Handgretinger Rupert , Fuchs Jörg , Warmann Steven W. , Schmid Evi 
Published: February 2022

Abstract
Purpose
In advanced tumor stages, pediatric rhabdomyosarcoma (RMS) shows an intrinsic resistance to standard chemotherapy, which is associated with a dismal prognosis. Alternative therapeutic approaches and optimization of already existent treatment protocols are urgently needed in these conditions. The µ-opioid receptor (OPRM1) agonist, D,L-methadone is frequently used for analgesia in oncological patients. Recent evidence has shown that D,L-methadone in combination with chemotherapeutic agents may enhance their cytotoxic effect against cancer cells. There are no related data in pediatric rhabdomyosarcoma (RMS).

Methods
Antitumor effects of combined D,L-methadone and doxorubicin, carboplatin, and vincristine on RMS cell lines RD and RH30 were analyzed using following outcome data: expression of the OPRM1 receptor (Western blot), cell growth inhibition (MTT assay), cell migration (wound-healing assay), apoptosis induction (caspase-3/7 assay), and reactive oxygen species (ROS) production (flow cytometry).

Results
In both cell lines, OPRM1 expression was significantly increased after combined treatment of D,L-methadone with all three cytotoxic drugs tested, which resulted in suppression of tumor cell growth and increase of apoptosis rates. These effects were mediated by increased ROS production and up-regulation of caspase-3/7 activity. Doxorubicin combined with D,L-methadone significantly reduced cell migration in both cell lines. Carboplatin or vincristine in combination with D,L-methadone had only an impact on cell migration in RH30 cells.

Conclusions
This new therapeutic approach in RMS provides strong antitumor effects in vitro. The combination of standard chemotherapy and D,L-methadone requires further investigation. Especially advanced tumors with a limited effectiveness of conventional treatment regimens seem a potential target of this approach.
Integrative analysis of the pharmaceutical active ingredient and transcriptome of the aerial parts of Glycyrrhiza uralensis under salt stress reveals liquiritin accumulation via ABA-mediated signaling
Authors: Bi Quan , Yao Hua , Wang Fei , He Dajun , Xu Wenbin , Xie Shuangquan , Chen Xifeng , Li Yuxia , Liu Hailiang , Shen Haitao , Li Hongbin 
Published: February 2022

AbstractThe aerial parts of Glycyrrhiza uralensis supply substantial raw material for the extraction of active pharmaceutical ingredients comprehensively utilized in many industries. Our previous study indicated that salt stress increased the content of active ingredients. However, the regulatory mechanism remains unclear. In this study, RNA-sequencing (RNA-seq) of the aerial parts of G. uralensis treated with 150 mM NaCl for 0, 2, 6, and 12 h was performed to identify the key genes and metabolic pathways regulating pharmacological active component accumulation. The main active component detection showed that liquiritin was the major ingredient and exhibited more than a ten-fold significant increase in the 6 h NaCl treatment. Temporal expression analysis of the obtained 4245 differentially expressed genes (DEGs) obtained by RNA-seq revealed two screened profiles that included the significant up-regulated DEGs (UDEGs) at different treatment points. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of these UDEGs identified phenylpropanoid metabolism and flavonoid biosynthesis as the most significantly enriched pathways in 2 h treated materials. Interestingly, the carotenoid biosynthesis pathway that is related to ABA synthesis was also discovered, and the ABA content was significantly promoted after 6 h NaCl treatment. Following ABA stimulation, the content of liquiritin demonstrated a significant and immediate increase after 2 h treatment, with the corresponding consistent expression of genes involved in the pathways of ABA signal transduction and flavonoid biosynthesis, but not in the pathway of glycyrrhizic acid biosynthesis. Our study concludes that salt stress might promote liquiritin accumulation through the ABA-mediated signaling pathway, and provides effective reference for genetic improvement and comprehensive utilization of G. uralensis.
Deep treatment-adaptive network for causal inference
Authors: Li Qian , Wang Zhichao , Liu Shaowu , Li Gang , Xu Guandong 
Published: February 2022

AbstractCausal inference is capable of estimating the treatment effect (i.e., the causal effect oftreatmenton theoutcome) to benefit the decision making in various domains. One fundamental challenge in this research is that the treatment assignment bias in observational data. To increase the validity of observational studies on causal inference, representation-based methods as the state-of-the-art have demonstrated the superior performance of treatment effect estimation. Most representation-based methods assume all observed covariates are pre-treatment (i.e., not affected by the treatment) and learn a balanced representation from these observed covariates for estimating treatment effect. Unfortunately, this assumption is often too strict a requirement in practice, as some covariates are changed by doing an intervention on treatment (i.e., post-treatment). By contrast, the balanced representation learned from unchanged covariates thus biases the treatment effect estimation. In light of this, we propose a deep treatment-adaptive architecture (DTANet) that can address the post-treatment covariates and provide a unbiased treatment effect estimation. Generally speaking, the contributions of this work are threefold. First, our theoretical results guarantee DTANet can identify treatment effect from observations. Second, we introduce a novel regularization of orthogonality projection to ensure that the learned confounding representation is invariant and not being contaminated by the treatment, meanwhile mediate variable representation is informative and discriminative for predicting the outcome. Finally, we build on the optimal transport and learn a treatment-invariant representation for the unobserved confounders to alleviate the confounding bias.
Making graphs compact by lossless contraction
Authors: Fan Wenfei , Li Yuanhao , Liu Muyang , Lu Can 
Published: February 2022

AbstractThis paper proposes a scheme to reduce big graphs to small graphs. It contracts obsolete parts and regular structures into supernodes. The supernodes carry a synopsis $$S_\mathcal {Q}$$

S
Q

for each query class $$\mathcal {Q}$$
Q
in use, to abstract key features of the contracted parts for answering queries of $$\mathcal {Q}$$
Q
. Moreover, for various types of graphs, we identify regular structures to contract. The contraction scheme provides a compact graph representation and prioritizes up-to-date data. Better still, it is generic and lossless. We show that the same contracted graph is able to support multiple query classes at the same time, no matter whether their queries are label based or not, local or non-local. Moreover, existing algorithms for these queries can be readily adapted to compute exact answers by using the synopses when possible and decontracting the supernodes only when necessary. As a proof of concept, we show how to adapt existing algorithms for subgraph isomorphism, triangle counting, shortest distance, connected component and clique decision to contracted graphs. We also provide a bounded incremental contraction algorithm in response to updates, such that its cost is determined by the size of areas affected by the updates alone, not by the entire graphs. We experimentally verify that on average, the contraction scheme reduces graphs by 71.9% and improves the evaluation of these queries by 1.69, 1.44, 1.47, 2.24 and 1.37 times, respectively.
Diagnostic delay in axial spondyloarthritis: a systematic review
Authors: Hay Charles A. , Packham Jon , Ryan Sarah , Mallen Christian D. , Chatzixenitidis Alexandros , Prior James A. 
Published: February 2022

AbstractIdentification of axial spondyloarthritis (axSpA) remains challenging, frequently resulting in a diagnostic delay for patients. Current benchmarks of delay are usually reported as mean data, which are typically skewed and therefore may be overestimating delay. Our aim was to determine the extent of median delay patients’ experience in receiving a diagnosis of axSpA and examine whether specific factors are associated with the presence of such delay. We conducted a systematic review across five literature databases (from inception to November 2021), with studies reporting the average time period of diagnostic delay in patients with axSpA being included. Any additional information examining associations between specific factors and delay were also extracted. A narrative synthesis was used to report the median range of diagnostic delay experienced by patients with axSpA and summarise which factors have a role in the delay. From an initial 11,995 articles, 69 reported an average time period of diagnostic delay, with 25 of these providing a median delay from symptom onset to diagnosis. Across these studies, delay ranged from 0.67 to 8 years, with over three-quarters reporting a median of between 2 years and 6 years. A third of all studies reported median delay data ranging from just 2 to 2.3 years. Of seven variables reported with sufficient frequency to evaluate, only ‘gender’ and ‘family history of axSpA’ had sufficient concordant data to draw any conclusion on their role, neither influenced the extent of the delay. Despite improvements in recent decades, patients with axSpA frequently experience years of diagnostic delay and this remains an extensive worldwide problem. This is further compounded by a mixed picture of the disease, patient and healthcare-related factors influencing delay.Key points• Despite improvements in recent decades, patients with axSpA frequently experience years of diagnostic delay.• Median diagnostic delay typically ranges from 2 to 6 years globally.• Neither ‘gender’ nor ‘family history of axSpA’ influenced the extent of diagnostic delay experienced.• Diagnostic delay based on mean, rather than median, data influences the interpretation of the delay time period and consistently reports a longer delay period.
Neuregelung der Hinzuverdienstmöglichkeiten für Transferbeziehende: keine leichte Aufgabe
Authors: Bruckmeier Kerstin , Wiemers Jürgen 
Published: February 2022

AbstractIn its coalition agreement, the German government proposes a reorganisation of the rules of earned income to be considered as a means test of basic income. In doing so, it takes up a frequently voiced criticism of the design of the basic income support for job-seekers under Book II of the Social Code. In recent years, researchers have called for a strengthening of (monetary) incentives to work, especially to take up employment with higher weekly working hours. In this paper, we summarise potential effects of such a reform on labour supply, the income distribution and the government budget in the context of a complex welfare system characterised by a high level of benefit non-take-up.
Bürgergeld und die Zukunft des Sozialstaats
Authors: Opielka Michael , Strengmann-Kuhn Wolfgang 
Published: February 2022

AbstractThe authors discuss the reform of basic security in the direction of a “citizen’s income” in the coalition agreement of the new “traffic light coalition” at the federal level against the background of fundamental considerations on the future of the welfare state, including those from the “Schleswig-Holstein Future Laboratory”. They see the reform of the citizen’s income as an incrementalist step toward a basic income and discuss their thesis on four topics: a) the question of the level of the basic income, b) the problem of counting other incomes, c) the relationship to the basic pension and citizen’s insurance, and d) the basic income for children.
Anhaltende Produktionslücken durch Vorleistungsengpässe
Authors: Bardt Hubertus , Grömling Michael 
Published: February 2022

ZusammenfassungDie anhaltenden Probleme bei der Beschaffung von Vorleistungen belasten die deutsche Wirtschaft. Drei Viertel der Unternehmen berichten derzeit von Produktionsausfällen von im Durchschnitt 7%. Erst ab dem zweiten Halbjahr 2022 wird sich die Situation deutlich verbessern. Aber auch im Jahr 2023 rechnet noch knapp die Hälfte der privatwirtschaftlichen Unternehmen mit Ausfällen. Neben den fehlenden Vorleistungen ist vor allem der Fachkräftemangel eine permanente Produktionsbremse.
3G am Arbeitsplatz: zur Situation in niedersächsischen Unternehmen Ende 2021
Authors: Thomsen Stephan L. , Trunzer Johannes 
Published: February 2022

ZusammenfassungDurch die Änderung des Infektionsschutzgesetzes Ende November 2021 wurde die Abbildung der 3G- und 2G-Quoten auf betrieblicher Ebene knapp ein Jahr nach Beginn der Impfungen möglich. Mithilfe einer Online-Blitzumfrage der Unternehmerverbände Niedersachsen, IHK Hannover, creditreform und dem Institut für Wirtschaftspolitik der Leibniz Universität Hannover zum Impf- und Genesenenstatus (2G), zur Zahl von Antigen-Schnelltests sowie dem Beschäftigtenanteil im Homeoffice können nun wichtige Hinweise auf die allgemeine Gefährdungslage im wirtschaftlichen und beruflichen Alltag sowie auf den damit verbundenen organisatorischen Aufwand in den Betrieben gegeben werden.
Migration patterns of Swedish Greylag geese Anser anser—implications for flyway management in a changing world
Authors: Månsson Johan , Liljebäck Niklas , Nilsson Lovisa , Olsson Camilla , Kruckenberg Helmut , Elmberg Johan 
Published: February 2022

AbstractSignificant population growth of some European goose populations has led to initiatives to implement management at the flyway level. Understanding migration routes and spatiotemporal distribution is crucial for the successful and coordinated management of migratory species such as geese. In this study, we describe movements across the entire annual cycle in 76 Greylag geese (Anser anser) fitted with GPS tracking devices at five catch sites in Sweden. We show that Greylag geese breeding in Sweden still use a NE-SW migration path. However, the wintering range has undergone a northward shift during the last decades. Compared to previous studies, our data suggest a continued reduction in migration distance, being most pronounced in birds in southernmost Sweden. Greylag geese tagged in southernmost Sweden spent almost the entire annual cycle in Sweden and Denmark (97 and 100% of all GPS locations). In contrast, the flyway of Greylag geese from the northern catch sites still covers countries from Sweden to Spain, but presently, only a small fraction of the population migrates to Spain. Instead, most of the annual cycle is spent in Sweden, Denmark, the Netherlands, or Germany. The contrasting spatiotemporal distribution in geese of different geographical origin indicates that management initiatives for the NW/SW European Greylag Goose population need to consider that different migration strategies occur within previously defined management units. As a consequence, coordination of management actions (e.g. monitoring, harvest quotas, reserves) may need to consider different spatial scales, i.e. from the regional to the international scale depending on the origin of the Greylag geese.
PrEP Use, Sexual Behaviour, and PrEP Adherence Among Men who have Sex with Men Living in Wales Prior to and During the COVID-19 Pandemic
Authors: Gillespie D. , Couzens Z. , de Bruin M. , Hughes D. A. , Jones A. , Ma R. , Williams A. , Wood F. , Blee K. , Bradshaw H. S. , Drayton R. , Knapper C. , Hood K. 
Published: February 2022

AbstractWe examined PrEP use, condomless anal sex (CAS), and PrEP adherence among men who have sex with men (MSM) attending sexual health clinics in Wales, UK. In addition, we explored the association between the introduction of measures to control transmission of SARS-CoV-2 on these outcomes. We conducted an ecological momentary assessment study of individuals in receipt of PrEP in Wales. Participants used an electronic medication cap to record PrEP use and completed weekly sexual behaviour surveys. We defined adherence to daily PrEP as the percentage of CAS episodes covered by daily PrEP (preceded by ≥ 3 days of PrEP and followed by ≥ 2 days). Sixty participants were recruited between September 2019 and January 2020. PrEP use data prior to the introduction of control measures were available over 5785 person-days (88%) and following their introduction 7537 person-days (80%). Data on CAS episodes were available for 5559 (85%) and 7354 (78%) person-days prior to and following control measures respectively. Prior to the introduction of control measures, PrEP was taken on 3791/5785 (66%) days, there were CAS episodes on 506/5559 (9%) days, and 207/406 (51%) of CAS episodes were covered by an adequate amount of daily PrEP. The introduction of pandemic-related control measures was associated with a reduction in PrEP use (OR 0.44, 95%CI 0.20–0.95), CAS (OR 0.35, 95%CI 0.17–0.69), and PrEP adherence (RR = 0.55, 95%CI 0.34–0.89) and this may have implications for the health and wellbeing of PrEP users and, in addition to disruption across sexual health services, may contribute to wider threats across the HIV prevention cascade.