bluuebunny/crossref_metadata_2025_split huggingface dataset:
Route of self-amplifying mRNA vaccination modulates the establishment of pulmonary resident memory CD8 and CD4 T cells
Authors: Künzli Marco , O’Flanagan Stephen D. , LaRue Madeleine , Talukder Poulami , Dileepan Thamotharampillai , Soerens Andrew G. , Quarnstrom Clare F. , Wijeyesinghe Sathi , Ye Yanqi , McPartlan Justine , Mitchell Jason S. , Mandl Christian W. , Vile Richard , Jenkins Marc K. , Ahmed Rafi , Vezys Vaiva , Chahal Jasdave , Masopust David 
Published: June 2022

AbstractRespiratory tract resident memory T cells (Trm), typically generated by local vaccination or infection, can accelerate control of pulmonary infections that evade neutralizing antibody. It is unknown whether mRNA vaccination establishes respiratory Trm. We generated a self-amplifying mRNA vaccine encoding the influenza A virus nucleoprotein that is encapsulated in modified dendron-based nanoparticles. Here we report how routes of immunization in mice, including contralateral versus ipsilateral intramuscular boosts, or intravenous and intranasal routes, influence influenza-specific cell-mediated and humoral immunity. Parabiotic surgeries revealed that intramuscular immunization was sufficient to establish CD8 Trm in lung and draining lymph node. Contralateral, compared to ipsilateral, intramuscular boosting broadened the distribution of LN Trm and T follicular helper cells, but slightly diminished resulting levels of serum antibody. Intranasal mRNA delivery established modest circulating CD8 and CD4 T cell memory, but augmented distribution to the respiratory mucosa. Of note, combining intramuscular immunizations with an intranasal mRNA boost achieved high levels of both circulating T cell memory and lung Trm. Thus, routes of mRNA vaccination influence humoral and cell-mediated immunity, and intramuscular prime-boosting establishes lung Trm that can be further enhanced by an additional intranasal immunization.
Niclosamide loaded Eudragit EPO nanoparticles show enhanced Candida biofilm penetration, trigger biofilm detachment and protect from mucosal candidiasis
Authors: Sutar Yogesh , Nabeela Sunna , Singh Shakti , Alqarihi Abdullah , Solis Norma , Ghebremariam Teklegiorgis , Filler Scott , Ibrahim Ashraf S. , Date Abhijit , Uppuluri Priya 
Published: June 2022

AbstractCandida albicans biofilms are a complex multilayer community of cells that are resistant to almost all classes of antifungal drugs. The bottommost layers of biofilms experience nutrient limitation where C. albicans cells are required to respire. We previously reported that a protein Ndu1 is essential for Candida mitochondrial respiration; loss of NDU1 causes inability of C. albicans to grow on alternative carbon sources and triggers early biofilm detachment. Here, we screened a repurposed library of FDA approved small molecule inhibitors, to identify those that prevent NDU1-associated functions. We identified an anti-helminthic drug, Niclosamide (NCL), which not only prevented growth on acetate, C. albicans hyphenation and early biofilm growth, but also completely disengaged fully grown biofilms of drug resistant C. albicans and C. auris from their growth surface. To overcome the sub-optimal solubility and permeability of NCL that is well-known to affect its in vivo efficacy, we developed NCL encapsulated Eudragit EPO (an FDA-approved polymer) nanoparticles (NCL-EPO-NPs) with high niclosamide loading, that also provided long-term stability. The developed NCL-EPO-NPs completely penetrated mature biofilms and attained anti-biofilm activity at low microgram concentrations. NCL-EPO-NPs induced ROS activity in C. albicans, and drastically reduced oxygen consumption rate in the fungus, similar to that seen in an NDU1 mutant. NCL-EPO-NPs also significantly abrogated mucocutaneous candidiasis by fluconazole resistant strains of C. albicans, in mice models of oropharyngeal and vulvovaginal candidiasis. To our knowledge, this is the first study that targets biofilm detachment as a target to get rid of drug-resistant Candida biofilms, and uses nanoparticles of an FDA approved non-toxic drug to improve biofilm penetrability and microbial killing.
The sulfur cycle connects microbiomes and biogeochemistry in deep-sea hydrothermal plumes
Authors: Zhou Zhichao , Tran Patricia Q. , Adams Alyssa M. , Kieft Kristopher , Breier John A. , Sinha Rupesh K. , Krishnan Kottekkatu P. , Kurian P. John , Fortunato Caroline S. , Sheik Cody S. , Huber Julie A. , Li Meng , Dick Gregory J. , Anantharaman Karthik 
Published: June 2022

AbstractIn globally distributed deep-sea hydrothermal vent plumes, microbiomes are shaped by the redox energy landscapes created by reduced hydrothermal vent fluids mixing with oxidized seawater. Plumes can disperse over thousands of kilometers and are complex. Their characteristics are determined by geochemical sources from hydrothermal vents, e.g., hydrothermal inputs, nutrients, and trace metals. However, the impacts of plume biogeochemistry on the oceans are poorly constrained due to a lack of integrated understanding of microbiomes, population genetics, and geochemistry. Here, we use microbial genomes to understand links between biogeography, evolution, and metabolic connectivity, and elucidate their impacts on biogeochemical cycling in the deep sea. Using data from 37 diverse plumes from 8 ocean basins, we show that sulfur metabolism defines the core microbiome of plumes and drives metabolic connectivity. Amongst all microbial metabolisms, sulfur transformations had the highest MW-score, a measure of metabolic connectivity in microbial communities. Our findings provide the ecological and evolutionary basis of change in sulfur-driven microbial communities and their population genetics in adaptation to changing geochemical gradients in the oceans.
TTBK2 controls cilium stability through actin and the centrosomal compartment
Authors: Nguyen Abraham , Goetz Sarah C. 
Published: June 2022

AbstractThe serine-threonine kinase Tau Tubulin Kinase 2 (TTBK2) is a key regulator of the assembly of primary cilia, which are vital signaling organelles. TTBK2 is also implicated in the stability of the assembled cilium, through mechanisms that remain to be defined. Here, we use mouse embryonic fibroblasts (MEFs) derived from Ttbk2fl/fl; UBC-CreERT+ embryos (hereafter Ttbk2cmut) to dissect the role of TTBK2 in cilium stability. This system depletes TTBK2 levels after cilia formation, allowing us to assess the molecular changes to the assembled cilium over time. As a consequence of Ttbk2 deletion, the ciliary axoneme is destabilized and primary cilia are lost within 48-72 hours following recombination. Axoneme destabilization involves an increased frequency of cilia breaks and is partially driven by altered actin dynamics and a reduction in axonemal microtubule modifications. At the same time, we find that TTBK2 is required to regulate the composition of the centriolar satellites and to maintain the basal body pools of intraflagellar transport (IFT) proteins. Altogether, our results reveal parallel pathways by which TTBK2 maintains cilium stability.
Combined small molecule treatment accelerates timing of maturation in human pluripotent stem cell-derived neurons
Authors: Hergenreder Emiliano , Zorina Yana , Zhao Zeping , Munguba Hermany , Calder Elizabeth L. , Baggiolini Arianna , Minotti Andrew P. , Walsh Ryan M. , Liston Conor , Levitz Joshua , Garippa Ralph , Chen Shuibing , Ciceri Gabriele , Studer Lorenz 
Published: June 2022

AbstractThe maturation of human pluripotent stem cell (hPSC)-derived neurons mimics the protracted timing of human brain development, extending over months and years to reach adult-like function. Prolonged in vitro maturation presents a major challenge to stem cell-based applications in modeling and treating neurological disease. We designed a high-content imaging assay based on morphological and functional readouts in hPSC-derived cortical neurons to reveal underlying pathways and to identify chemicals capable of accelerating neuronal maturation. Probing a library of 2688 bioactive drugs, we identified multiple compounds that drive neuronal maturation including inhibitors of LSD1 and DOT1L and activators of calcium-dependent transcription. A cocktail of 4 factors GSK-2879552, EPZ-5676, NMDA and Bay K 8644, which we collectively termed GENtoniK, triggered maturation across all assays tested including measures of synaptic density, electrophysiology and transcriptomics. Remarkably, GENtoniK was similarly effective in enhancing neuronal maturation in 3D cortical organoids and in spinal motoneurons, and improved aspects of cell maturation in non-neural lineages such as melanocytes and pancreatic beta cells. These results demonstrate that the maturation of multiple hPSC-derived cell types can be enhanced by simple pharmacological intervention and suggests that some of the mechanisms controlling the timing of human maturation are shared across lineages.
Exploring how entrepreneurial orientation improve firm resilience in digital era: findings from sequential mediation and FsQCA
Authors: Xia Qinghua , Xie Yi , Hu Shuchuan , Song Jianmin 
Published: June 2022

PurposeUnder extensive pressure from normal market competition, frequent technological change and extreme exogenous shock, firms are facing severe challenge nowadays. How to withstand discontinuous crises and respond to normal risks through improving resilience (RE) is an important question worth researching. Thus, drawing on the strategic entrepreneurship theory, the purpose of this study is exploring the relationship between entrepreneurial orientation (EO) and RE, and combining digitization to discuss the role of digital business capability (DBC), digital business model innovation (DBMI) and environmental hostility (EH).Design/methodology/approachBased on survey data from 203 Chinese firms, using the methods of linear regression and bootstrap to test our hypothesis. Furthermore, fuzzy-set qualitative comparative analysis (FsQCA) is used to identify previously unknown combinations which lead to strong/weak RE in digital context.FindingsFirst, EO positively influenced DBC and RE. Second, DBMI promoted RE, DBC and DBMI served as sequential mediators that linked EO and RE. Third, EH positively moderated the effects of EO on RE. Further the study revealed that different configuration of DBMI and dimensions of EO and DBC can explain RE.Originality/valueThe study explains mechanism of RE from perspective of digitization. The conclusion is good for further consolidating strategic entrepreneurship theory, and providing a new frame for firms to build the ability of antifragile.
An intelligent optimization technique for performance improvement in radial distribution network
Authors: Vempalle Rafi , Pradyumna Kumar Dhal 
Published: June 2022

PurposeThe demand for electricity supply increases day by day due to the rapid growth in the number of industries and consumer devices. The electric power supply needs to be improved by properly arranging distributed generators (DGs). The purpose of this paper is to develop a methodology for optimum placement of DGs using novel algorithms that leads to loss minimization.Design/methodology/approachIn this paper, a novel hybrid optimization is proposed to minimize the losses and improve the voltage profile. The hybridization of the optimization is done through the crow search (CS) algorithm and the black widow (BW) algorithm. The CS algorithm is used for finding some tie-line systems, DG locations, and the BW algorithm is used for finding the rest of the tie-line switches, DG sizes, unlike in usual hybrid optimization techniques.FindingsThe proposed technique is tested on two large-scale radial distribution networks (RDNs), like the 119-bus radial distribution system (RDS) and the 135 RDS, and compared with normal hybrid algorithms.Originality/valueThe main novelty of this hybridization is that it shares the parameters of the objective function. The losses of the RDN can be minimized by reconfiguration and incorporating compensating devices like DGs.
Fanning the flames: understanding viral content after brand transgressions
Authors: Legocki Kimberly V. , Walker Kristen L. , Eilert Meike 
Published: June 2022

Purpose
This paper aims to contribute to the emerging body of research on firestorms, specifically on the inflammatory user-generated content (UGC) created in response to brand transgressions. By analyzing and segmenting UGC created and shared in the wake of three different events, the authors identify which type of inflammatory message is most likely to be widely shared; thus, contributing to a possible online firestorm.

Design/methodology/approach
Tweets were collected involving brand transgressions in the retail, fast food and technology space from varying timeframe and diverse media coverage. Then, the tweets were coded for message intention and analyzed with linguistics software to determine the message characteristics and framing. A two-step cluster analysis identified three types of UGC.

Findings
The authors found that message dimensions and the framing of tweets in the context of brand transgressions differed in characteristics, sentiment, call to action and the extent to which the messages were shared. The findings contradict traditional negative word-of-mouth studies involving idiosyncratic service and product failure. During online brand firestorms, rational activism messages with a call to action, generated in response to a firm’s transgression or “sparks,” have a higher likelihood of being shared (virality).

Originality/value
This research provides novel insights into UGC created after brand transgressions. Different types of messages created after these events vary in the extent that they “fan the flames” of the transgression. A message typology and flowchart are provided to assist managers in identifying and responding to three message types: ash, sparks and embers.
Does size matter? Is there an optimal size for tenant–owner associations?
Authors: Karpestam Peter , Palm Peter 
Published: June 2022

PurposeThe authors investigate how prices of condominiums are affected by the size of the tenant-owner associations that they belong to.Design/methodology/approachThe authors use data of sold apartments in the Swedish municipality Malmö 2013–2018 and estimate hedonic price regressions. The authors also perform semi-structured interviews with three senior professionals in real estate companies.FindingsThe authors find significantly negative relationships between the prices of condominiums and the size of tenant-owner associations. Also, regression results indicate that associations should be no smaller than 6–10 apartments. The interviews support that associations should not be too small or too big. The lower and upper limit was suggested by the respondents to 40–50 and 80–150 apartments, respectively. In these ranges, economies of scale can be achieved, and residents will not lose the sense of community and responsibility.Research limitations/implicationsThe authors do not prove causality. Smaller associations may have relatively exclusive common amenities, about which we lack data. The same relationships may not exist in different market conditions.Originality/valueThe authors are not aware of previous studies with the same research question. The size of tenant-owner associations may affect the price through different channels. First, several of the banks in Sweden do not always grant mortgages for condominiums that belong to small associations. Second, larger associations may have better economies of scale and more efficient property management. Third, homeowners may prefer smaller tenant-owned associations, because they may feel less anonymous and provide more influence on common amenities.
A systematic review on forensic accounting and its contribution towards fraud detection and prevention
Authors: Kaur Baljinder , Sood Kiran , Grima Simon 
Published: June 2022

Purpose
This paper aims to determine how forensic accounting contributes to fraud detection and prevention and answer the following research questions: What are the standard techniques for fraud detection and prevention; and What are the significant challenges that hinder the application of forensic accounting in fraud prevention and detection?

Design/methodology/approach
The authors use the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method to carry out a systematic literature review (SLR) to identify and assess the existing literature on forensic accounting.

Findings
There exists a positive correlation between forensic accounting and fraud detection and prevention. Moreover, in both the empirical and non-empirical findings, the authors note that fraud is complex, and in carrying out fraud investigations, one must be aware of its complexity.

Practical implications
Although drug counterfeiting is a sector where forensic accountants have paid less attention, it is a rapidly expanding fraud area. This paper finds that to detect fraud at an early stage, one must increase consumer understanding of basic forensic accounting techniques by implementing accurate supply chain monitoring systems and inventory management controls and conducting adequate and effective regulatory, honest and legitimate customs inspections.

Social implications
The major factors that restrict forensic accounting are a lack of awareness and education. Hence, it is essential to incorporate forensic accounting in undergraduate and post-graduate courses.

Originality/value
From the existing literature, it has been observed that very few studies have been conducted in this field using the PRISMA and SLR techniques. Also, the authors carried out a holistic study that focuses on three different areas – fraud detection, fraud prevention and the challenges in forensic accounting.
A scale for measuring healthcare service quality incorporating patient-centred care and using a psychometric analytics framework
Authors: Rave Jorge Iván Pérez , Sánchez Figueroa Génesis Angélica , González Echavarría Favián 
Published: June 2022

PurposeRecent developments in healthcare contexts increasingly emphasize patient-centred approaches to service quality measures; however, few studies consider this dimension explicitly. The present study develops and psychometrically validates a scale of healthcare service quality explicitly incorporating a patient-centred care dimension from a communicational perspective. The paper also enriches the traditional content of service quality by including equity items and presents the underlying structure of service quality in an emerging country.Design/methodology/approachThe final sample consisted of 869 healthcare users (complete cases in the service quality items derived from 917 surveys received) from Colombia. The authors used a psychometric analytics framework comprising seven processes incorporating exploratory factor analysis, structural equation modelling, and machine learning methods to examine construct plausibility, reliability, construct validity, equity, and criterion/predictive validity (e.g. explaining/predicting subjective well-being and behavioural intentions).FindingsThe final scale consists of 17 items and satisfies all psychometric properties. Its validation allows for the discovery and psychometrical confirmation of two essential dimensions: patient-centred communication (eight items) and process quality (nine items).Practical implicationsThe authors illustrate three practical uses of the scale: the possibility for diagnoses; hypothesis contrast based on confidence intervals; and estimation of the capacity of the service to satisfy specifications.Originality/valueBoth dimensions reveal users' relevant needs and complement previous studies that have focused on process aspects of healthcare service quality.
Design and development of digital information product and service using colour QR codes for smartphone user's of academic libraries
Authors: Kharat Santosh Abaji , Nagarkar Shubhada , Panage Bhausaheb Murlidhar 
Published: June 2022

PurposeThe objectives of the study include the following: (1) to design and develop digital information products by using Quick Response (QR) codes (2) to make an awareness of QR codes in library users; (3) to study user preferences for black and green QR codes in the library services of management institutes and (4) to raise awareness of the product layer among the library profession.Design/methodology/approachThis study uses a participatory action research approach. The researcher implemented QR codes in the library, and participants scanned QR codes through their smartphones. All data analyses were performed using IBM SPSS and Microsoft Excel 2016.FindingsThe researcher found that both QR codes will be popular in the future; however, users will prefer to scan the green QR code multiple times due to the code's increased benefits and advantages (which have been discussed in this report).Research limitations/implicationsThis study implemented QR codes in MBA libraries affiliated with Savitribai Phule Pune University in the Pune and Pimpri Chinchwad areas.Originality/valueThe current study implemented a QR code in the library service with green and black colours. This study examined which QR code will be popular in the future owing to QR code's advanced features and specifications. This, in turn, will assist the library in designing such a collection and developing more QR codes that will be recommended to users. This will assist in the creation of good digital information products for smartphone users.
Women on boards, firms’ competitive advantage and its effect on ESG disclosure in Malaysia
Authors: Wan Mohammad Wan Masliza , Zaini Rapiah , Md Kassim Aza Azlina 
Published: June 2022

Purpose
The purpose of this paper is to investigate the effects of women on board moderated by firms’ competitive advantage on firms’ environmental, social and governance (ESG) disclosures.

Design/methodology/approach
The sample consists of 332 firm-year observations from the year 2012 to 2017 of 65 firms listed in Bursa Malaysia. To improve the robustness of this analysis, the authors adopt clustering techniques in the regression analysis. Sensitivity analysis is also conducted using two-stage least square regression and robust standard errors for panel regression with a cross-sectional dependence approach.

Findings
The findings of this research indicate that women on board encourage ESG and environmental disclosures. Nonetheless, in competitively advantaged firms, the authors find that the interaction between WOMENPER and COMADVANTAGE is negatively influencing ESG scores. However, no evidence is found to indicate that women on board in a competitively advantaged firm have an effect on the environmental scores of a firm.

Research limitations/implications
The findings urge regulators to ensure the appointment of qualified and competent women on board, particularly in competitively advantage firms.

Practical implications
Though firms with more women on board are associated with better ESG disclosures and environmental disclosures, the author’s additional analysis found that this is less pronounced in competitively advantage firms. Since a number of the competitive firms are owned by family firms as well as government-linked firms, the appointment of women should not be based on directors’ affiliation, network and family relationships.

Originality/value
To the best of authors’ knowledge, this is one of the few studies which seek to investigate women’s appointment in competitive advantage firms.