bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of Brazilian grape juice metabolomic profile changes caused by methyl jasmonate pre‐harvest treatment
Authors: Moro Laís , da Mota Renata Vieira , Purgatto Eduardo , Mattivi Fulvio , Arapitsas Panagiotis 
Published: June 2022

SummaryGrape juice is a major source of potential health‐promoting bioactive polyphenols, especially for children and those who do not consume wine. Since the subtropical climate may negatively affect the concentrations of grape polyphenols, especially anthocyanins, elicitors such as methyl jasmonate (MeJa) could be used to promote polyphenol biosynthesis. This work aimed at investigating the impact of MeJa treatment on grape juice produced via a traditional low‐cost process from two Vitis labrusca cultivars and in two Brazilian regions. The untargeted LC–MS analytical protocol demonstrated that Isabel Precoce juices strongly benefited from MeJa treatment, especially regarding their anthocyanic profile, regardless of the cultivation region. Known MeJa markers in wine and V. vinifera grapes (flavanols, flavonols and stilbenes) in this experiment had mixed behaviours depending on the region/variety/cultivation. Moreover, it was found that all the detected hydroxycinnamates were influenced by the treatment, especially the concentration of their glucosides, which was increased. Glutathione, 2‐S‐glutathionyl caftaric acid and indole lactic acid glucoside were identified for the first time as MeJa treatment biomarkers in grape products, indicating a possible positive effect on juice antioxidant properties.
Comparative assessment of bone collagen recovered from different livestock and poultry species: microstructure, physicochemical characteristics and functional properties
Authors: Liu Hong , Guo Yujie , Xu Xiong , Liu Jiqian , Zhang Hongru , Qi Liwei , Zhang Chunhui , Gao Hongwei 
Published: June 2022

SummaryThis study aimed to recover and characterise livestock and poultry bone collagens (LBCs) from yak (YBC), bovine (BBC), porcine (PBC) and chicken (CBC) sources and perform comparative assessment on their microstructure, physicochemical and functional properties. Amino acid compositions, SDS–PAGE and spectroscopic analysis confirmed that LBCs were intact triple‐helical collagen I of [α1(I)]2α2(I) with some structural differences. Scanning electron microscope (SEM) results showed that LBCs displayed a loose and porous fibrous meshwork. The Td values of SCI, YBC, BBC, PBC and CBC were 42.6, 42.3, 44.6, 41.9 and 42.9 °C, and 5.81, 7.15, 6.28, 6.79 and 5.33 of pI values, respectively. Structural differences also endowed LBCs with dissimilar functional properties, such as water‐holding capacity, foaming and emulsifying. The structure–function correlations analysis revealed the structure–function correlations of LBCs. This study offered a new prospect that the sustainable livestock and poultry bones (LBs) might be valorised as an alternative collagen source and provided useful information for the industrial production and fostered further rational valorisation of LBCs.
Effects of Agaricus bisporus on gel properties of chicken myofibrillar protein
Authors: Nan Haijuan , Stepanova Tetiana M. , Kondratiuk Natalia V. , Nie Yuanyang , Li Bo 
Published: June 2022

SummaryThe gel‐forming ability of myofibrillar protein (MP) is highly correlated with the characteristics of emulsified meat products. Incorporation of Agaricus bisporus (Ab) powder into MP gels may enhance its gel properties to facilitate the development of a novel and safe meat product. Therefore, this study investigated the effects of Ab powder on gel strength, water holding capacity (WHC), texture, rheological behaviour, LF‐NMR spin–spin relaxation (T2), microstructure and protein secondary structure of the MP gel system. The results indicated that the gel strength, WHC, G' value and G" value were significantly improved when the addition of Ab powder increased from 0% to 6% (P < 0.05). Meanwhile, the T2 relaxation time was shortened, and free water was transformed into immobilised water. The texture of the gel was improved when 1%–4% Ab powder was added compared to the control. Furthermore, Ab filled in the gel network and promoted the unfolding of MP α‐helix and the formation of MP β‐sheet during the thermal denaturation of MP, leading to a dense aggregated network structure. The study suggested that Ab could be a promising ingredient in improving chicken MP's gel properties and developing fat‐reduced meat products.
Effect of combined treatment including Litsea cubeba essential oil and wood smoke on Aspergillus niger development and consumer acceptability of dried catfish (Ariidae)
Authors: Sinthupachee Apiram , Koomhin Phanit , Matan Nirundorn , Matan Narumol 
Published: June 2022

SummaryDried fish exports generate massive revenue. These exports are in highest demand in the world market. However, dried fish can quickly become spoiled by mould. This creates foul odours that may affect consumer acceptance. Dried fish requires natural antimicrobial agents to improve shelf life. This study aimed to control mould growth and improve the organoleptic properties of dried fish using Litsea cubeba oil vapour in combination with wood smoke. In addition, to understand consumer acceptability, the treated dried fish was used to test consumer brain responses using electroencephalography (EEG). The participants who were able to eat dried fish preferred smoked food and had no history of allergies to dried fish, and essential oils were selected for this test. The combined treatment of the L. cubeba oil vapour at ≥60 μL and smoke for 30 s completely controlled the growth of A. niger at least 6 Log10 CFU g−1 and extended the shelf life of dried fish to 7 days at 30 ± 2 °C compared with the control (3 days). The panellists preferred the dried fish treated with the combined treatment more than the untreated fish (P < 0.05). The consumption (smelling, eating and swallowing) of the treated fish by the participants increased the alpha wave activity in their brains to increase the activation of the neural circuits for attention, working memory, active stage and sensory‐motor integration. These findings can be helpful for smoke‐dried fish product development and dried fish preservation.
Acrylamide: impact of precursors concentration, origin, post‐harvesting process and roasting level in high‐quality arabica and Robusta coffee
Authors: Vezzulli Fosca , Triachini Sara , Mulazzi Annalisa , Lambri Milena , Bertuzzi Terenzio 
Published: June 2022

SummaryOrigin of coffee, precursors concentration, post‐harvesting processes and commercial roasting degrees were evaluated for their impact on acrylamide content in roasted coffee. Forty Seven Specialty Arabica and 7 high‐quality Robusta green coffee samples were analysed to determine sugars, asparagine and Aw. Acrylamide was quantified on light, medium and dark roasted samples. In green coffee, glucose and fructose content resulted lower after wet and honey processes, no remarkable differences were found for sucrose and asparagine. In all samples, the content of asparagine was generally lower than what provided in previous works. Acrylamide concentration never exceeded the limit of 400 μg kg−1 in Arabica samples and it does once in Robusta, it peaked between light and medium roasting, and it was higher in Robusta. Moreover, it was lower in honey coffee than in others. Acrylamide correlated with asparagine in Robusta, while with monosaccharides and Aw in dry and honey Arabica. Coffee Origin impacted on precursors and acrylamide.
Comparison of perioperative outcomes among patients with exophytic, mesophytic, and endophytic renal tumors undergoing robot‐assisted partial nephrectomy
Authors: Motoyama Daisuke , Ito Toshiki , Sugiyama Takayuki , Otsuka Atsushi , Miyake Hideaki 
Published: June 2022

ObjectivesIt has been well documented that partial nephrectomy for completely endophytic renal tumors is a highly challenging procedure accompanied by several technical difficulties even with the assistance of a robotic surgical system. This study aimed to compare perioperative variables among patients with exophytic, mesophytic, and endophytic renal tumors undergoing robot‐assisted partial nephrectomy.MethodsThis study retrospectively included 265 consecutive patients with localized small renal masses undergoing robot‐assisted partial nephrectomy at our institution. In this study, completely endophytic tumor was defined as the mass totally covered by renal healthy parenchyma, and according to the points for the ‘E' domain of RENAL nephrometry score based on preoperative computed tomography, subjects were classified into three groups as follows: exophytic, mesophytic, and endophytic tumor groups, and perioperative outcomes among these groups were compared.ResultsOf 265 patients, 127, 112, and 26 were classified into the exophytic, mesophytic, and endophytic tumor groups, respectively. A significantly smaller tumor diameter was observed in the endophytic group than in the other groups (P < 0.001), whereas the RENAL nephrometry score was significantly higher (P < 0.001). In addition, the warm ischemia time in the endophytic tumor group was significantly longer than that in other groups (P = 0.009); however, no significant difference in the trifecta achievement was noted among the three groups.ConclusionsThis study suggests that robot‐assisted partial nephrectomy for patients with completely endophytic tumors can be regarded as a feasible approach without marked impairment of perioperative outcomes; however, further investigation of the long‐term functional and oncological outcomes in these patients is required.
Corporate social responsibility and corporate tax avoidance: The channel effect of consumer awareness
Authors: Benlemlih Mohammed , Jaballah Jamil , Schochet Sholom , Peillex Jonathan 
Published: June 2022

AbstractThis paper investigates the relationship between corporate social responsibility (CSR) and corporate tax avoidance and the impact of consumer awareness on the constructs. We show that consumer awareness has the effect of decreasing the positive relation between CSR ratings and tax avoidance levels. Our findings are robust to various measures of our constructs. Collectively, the findings of this study lend credence to the risk‐management view of CSR and demonstrate that consumer awareness acts as a behavioral intervention and governance mechanism to help mitigate corporate hypocrisy.
LGR5 regulates osteogenic differentiation of human thoracic ligamentum flavum cells by Wnt signalling pathway
Authors: Yang Xiaoxi , Sun Chuiguo , Meng Xiangyu , Chen Guanghui , Fan Tianqi , Zhang Chi , Chen Zhongqiang 
Published: June 2022

AbstractThoracic ossification of the ligamentum flavum (TOLF) is ectopic ossification of the spinal ligaments. Histologically, the development of TOLF can be described as the process of endochondral ossification. However, the underlying aetiology has not been completely clarified. In this investigation, the gene expression profile associated with leucine‐rich repeat‐containing G‐protein‐coupled receptors (LGR) and Wnt signalling pathway in the thoracic ligamentum flavum cells (TLFCs) of different ossification stages was analysed via RNA sequencing. We further confirmed the significant differences in the related gene expression profile by Gene Ontology (GO) enrichment analysis. LGR5 was first identified in primary human TLFCs during osteogenic differentiation. To evaluate the effect of LGR5 on osteogenic differentiation, LGR5 has been knocked down and overexpressed in human TLFCs. We observed that the knockdown of LGR5 inhibited the activity of Wnt signalling and attenuated the potential osteogenic differentiation of TLFCs, while overexpression of LGR5 activated the Wnt signalling pathway and increased osteogenic differentiation. Our results provide important evidence for the potent positive mediatory effects of LGR5 on osteogenesis by enhancing the Wnt signalling pathway in TOLF.
A national study of disenrollment from hospice among people with dementia
Authors: Hunt Lauren J. , Gan Siqi , Boscardin W. John , Yaffe Kristine , Ritchie Christine S. , Aldridge Melissa D. , Smith Alexander K. 
Published: June 2022

AbstractBackgroundPeople with dementia (PWD) are at high risk for hospice disenrollment, yet little is known about patterns of disenrollment among the growing number of hospice enrollees with dementia.DesignRetrospective, observational cohort study of 100% Medicare beneficiaries with dementia aged 65 and older enrolled in the Medicare Hospice Benefit between July 2012 and December 2017. Outcome measures included hospice‐initiated disenrollment for patients whose rate of decline ceased to meet the Medicare hospice eligibility guideline of “expected death within 6 months” (extended prognosis) and patient‐initiated disenrollment (revocation). Hospice, regional, and patient risk factors and variation were assessed with multilevel mixed‐effects logistic regression models.ResultsAmong 867,695 hospice enrollees with dementia, 70,945 (8.2%) were disenrolled due to extended prognosis and 43,133 (5.0%) revoked within 1‐year of their index admission. There was substantial variation in hospice provider disenrollment due to extended prognosis (10th–90th percentile 4.5%–14.6%, adjusted median odds ratio (MOR) 1.86, 95% confidence interval (CI) 1.82, 1.91) and revocation (10th–90th percentile 2.5%–10.1%, MOR 2.09, 95% CI 2.03, 2.14). Among hospital referral regions (HRR), there was more variation in revocation (10th–90th percentile 3.5%–7.6%, MOR 1.4, 95% CI 1.34, 1.47) than extended prognosis (10th–90th percentile 7.0%–9.5%, MOR 1.23, 95% CI 1.18, 1.27), with much higher revocation rates noted in HRRs located in the Southeast and Southern California. A number of patient and hospice characteristics were associated with higher odds of both types of disenrollment (younger age, female sex, minoritized race and ethnicity, Medicaid dual eligibility, Medicare Part C enrollment), while some were associated with revocation only (more comorbidities, newer, smaller, and for‐profit hospices).ConclusionsIn this nationally representative study of hospice enrollees with dementia, hospice disenrollment varied by type of hospice, geographic region, and patient characteristics including age, sex, race, and ethnicity. These findings raise important questions about whether and how the Medicare Hospice Benefit could be adapted to reduce disparities and better support PWD.
Geriatric surgical service interventions in older emergency general surgery patients: Preliminary results
Authors: Hu Frances Y. , O'Mara Lynne , Tulebaev Samir , Orkaby Ariela R. , Cooper Zara , Bernacki Rachelle E. 
Published: June 2022

AbstractBackgroundOlder adults comprise an increasing proportion of emergency general surgery (EGS) admissions and face high morbidity and mortality. We created a geriatric surgical service with geriatric and palliative expertise to mitigate risks of hospitalization most hazardous to older patients. We sought to identify geriatric surgical service interventions most relevant to EGS patients.MethodsWe prospectively identified patients ≥75 years admitted to the EGS service at an urban tertiary care hospital from January 2020–March 2021 who screened positive for frailty (FRAIL score ≥3 [scale 0–5, higher being worse]) or with cognitive impairment. A pilot geriatric surgical service, led by a dually‐board certified geriatric and palliative care specialist, conducted a comprehensive geriatric assessment and modified Rockwood Frailty Index calculation for each eligible patient. Patient, hospital admission, and geriatric consultation characteristics were collected via chart review.ResultsFifty consecutive patients (median age 82 years [IQR 78–90], 56% female) received geriatric consultation (median time 3 days [IQR 1–6] from admission). The most common admission diagnosis was bowel obstruction (32%). Sixty‐four percent of patients underwent ≥1 surgical procedure. Using the Frailty Index, 64% were moderately or severely frail. Interventions most frequently performed by the geriatric team included delirium prevention and management (66%), consideration of swallowing function (52%), individualized pain management (50%), and facilitation of serious illness conversations (58%).ConclusionsGeriatric service involvement addresses a high burden of both geriatric and palliative care needs in older EGS patients. Geriatric recommendations may direct interventions for surgical education in fundamental geriatric and palliative care knowledge to maximize geriatric resources for the most high‐risk patients.
Associations of plasma betaine, plasma choline, choline intake, and MTHFR polymorphism (rs1801133) with anthropometric parameters of healthy adults are sex‐dependent
Authors: Mlodzik‐Czyzewska Monika A. , Malinowska Anna M. , Szwengiel Artur , Chmurzynska Agata 
Published: June 2022

AbstractBackgroundCholine and its metabolites apppear to have relationships with body mass index (BMI), body fat, and body weight, but the research results have proved inconsistent. We thus investigated the associations of plasma levels of trimethylamine N‐oxide (TMAO), choline, and betaine with anthropometric measurements, including modulatory effects of genetics and diet.MethodsThe study was performed on a group of 421 adults, aged 20–40 years, who had been recruited in Poland. Plasma concentrations of choline, betaine, and TMAO were determined using reverse‐phase ultra‐high‐performance liquid chromatography electrospray ionisation mass spectrometry. The following polymorphisms were genotyped using TaqMan probes: rs180113 (MTHFR), rs70991108 (DHFR), rs2236225 (MTHFD1), and rs7946 and rs12325817 (PEMT). We employed multivariate linear regression to examine the associations between anthropometric measurements, one‐carbon metabolism metabolites, and genotypes.ResultsHigher plasma choline was associated with higher BMI (β = 0.17; p < 0.01), body weight (β = 0.11; p < 0.05), body fat mass (FM) (β = 0.10; p < 0.05), and waist circumference (WC) (β = 0.14; p < 0.01), whereas higher choline intake was associated with lower body FM (β = −0.14; p < 0.01) and lower WC (β = −0.12; p < 0.01). After stratification by sex, plasma betaine was found to be associated with lower BMI (β = −0.20; p < 0.05) and body weight (β = −0.16; p < 0.05) in men only, whereas choline intake was associated with lower body FM (β = −0.19; p < 0.05) and waist‐to‐hip ratio (WHR) (β = −0.19; p < 0.05) and MTHFR CC genotype was associated with WHR (β = 0.15; p < 0.05) in women only.ConclusionsHigher plasma betaine and higher dietary choline are associated with lower FM and body weight, whereas higher plasma choline is positively associated with body weight status and adiposity. Moreover, these associations appear to be sex‐specific.
The effectiveness of music interventions on stress response in intensive care patients: A systematic review and meta‐analysis
Authors: Erbay Dalli Öznur , Bozkurt Canan , Yildirim Yasemin 
Published: June 2022

AbstractAim and objectiveTo investigate the effectiveness of music interventions on physiological and psychological stress response in intensive care unit (ICU) patients.BackgroundICU patients are exposed to several physiological and psychological stressors during their treatments. Music interventions can be implemented to control these negative effects.DesignSystematic review and meta‐analysis conducted according to PRISMA Guidelines.MethodSeven electronic literature databases, reference lists of similar reviews, grey literature and the Clinical Trials Registry were searched for potential studies up to 30 January 2022. Studies were expected to meet PICOS inclusion criteria. Two reviewers independently assessed the risk of bias of the included studies by using the Cochrane Collaboration tool. Overall, meta‐analysis and subgroup analyses (comparator group and music session frequency) were performed using RevMan 5.4. Meta‐analysis was conducted when data were available; otherwise, a narrative description was provided.ResultsTwenty‐five articles were included in this meta‐analysis. Across all studies, regardless of the comparator, music was found to significantly reduce anxiety scores with an SMD of 1.81 ([95% CI: −3.09,‐0.53]; p = .006). Music was found to significantly reduce anxiety scores with an SMD of 1.97 (95% CI: −3.66, −0.28) compared to standard care (p = .02), but there was no significant change in anxiety scores in comparison with the noise cancellation group (p > 0.05). It was found that multiple music sessions reduced the anxiety level better than a single music session. Music had no effect the systolic/diastolic blood pressure, respiratory or heart rate level in both subgroup and general analyzes concerning the physiological stress (p > 0.05).ConclusionsMusic interventions involving multiple sessions can be used as a nursing intervention to control the anxiety levels of ICU patients.Relevance to the clinical practiceUsing music to reduce anxiety and stress levels may reduce the pharmacological need (for sedative or antipsychotic medications) and the risk of associated side effects in ICU patients.
Lipolysis defect in people with obesity who undergo metabolic surgery
Authors: Rydén Mikael , Andersson Daniel P. , Kotopouli Maria I. , Stenberg Erik , Näslund Erik , Thorell Anders , Sørensen Thorkild I. A. , Arner Peter 
Published: June 2022

AbstractObjectiveCross‐sectional studies demonstrate that catecholamine stimulation of fat cell lipolysis is blunted in obesity. We investigated whether this defect persists after substantial weight loss has been induced by metabolic surgery, and whether it is related to the outcome.Design/MethodsPatients with obesity not able to successfully reduce body weight by conventional means (n = 126) were investigated before and 5 years after Roux‐en‐Y gastric bypass surgery (RYGB). They were compared with propensity‐score matched subjects selected from a control group (n = 1017), and with the entire group after adjustment for age, sex, body mass index (BMI), fat cell volume and other clinical parameters. Catecholamine‐stimulated lipolysis (glycerol release) was investigated in isolated fat cells using noradrenaline (natural hormone) or isoprenaline (synthetic beta‐adrenoceptor agonist).ResultsFollowing RYGB, BMI was reduced from 39.9 (37.5–43.5) (median and interquartile range) to 29.5 (26.7–31.9) kg/m2 (p < 0.0001). The post‐RYGB patients had about 50% lower lipolysis rates compared with the matched and total series of controls (p < 0.0005). Nordrenaline activation of lipolysis at baseline was associated with the RYGB effect; those with high lipolysis activation (upper tertile) lost 30%–45% more in body weight, BMI or fat mass than those with low (bottom tertile) initial lipolysis activation (p < 0.0007).ConclusionPatients with obesity requiring metabolic surgery have impaired ability of catecholamines to stimulate lipolysis, which remains despite long‐term normalization of body weight by RYGB. Furthermore, preoperative variations in the ability of catecholamines to activate lipolysis may predict the long‐term reduction in body weight and fat mass.