bluuebunny/crossref_metadata_2025_split huggingface dataset:
Herbarium phylogenomics: Resolving the generic status of the enigmatic Pseudobartsia (Orobanchaceae)
Authors: Jiang Nan , Dong Li‐Na , Yang Jun‐Bo , Tan Yun‐Hong , Wang Hong , Randle Christopher P. , Li De‐Zhu , Yu Wen‐Bin 
Published: March 2022

AbstractThe millions of herbarium specimens in collections around the world provide historical resources for phylogenomics and evolutionary studies. Many rare and endangered species exist only as historical specimens. Here, we report a case study of the monotypic Pseudobartsia yunnanensis D. Y. Hong (=Pseudobartsia glandulosa[Bentham] W. B. Yu & D. Z. Li: Orobanchaceae) known from a single Chinese collection taken in 1940. We obtained genomic data of Pseudobartsia glandulosa using high‐throughput short‐read sequencing, and then assembled a complete chloroplast genome and nuclear ribosome DNA region in this study. We found that the newly assembled three plastid DNA regions (atpB‐rbcL, rpl16, and trnS‐G) and nuclear ribosomal internal transcribed spacer (nrITS) of Pseudobartsia glandulosa were more than 99.98% similar to published sequences obtained by target sequencing. Phylogenies of Orobanchaceae using 30 plastomes (including 10 new plastomes), using both supermatrix and multispecies coalescent approaches following a novel plastid phylogenomic workflow, recovered seven recognized tribes and two unranked groups, both of which were proposed as new tribes, that is, Brandisieae and Pterygielleae. Within Pterygielleae, all analyses strongly supported Xizangia D. Y. Hong as the first diverging genus, with Pseudobartsia D. Y. Hong as sister to Pterygiella Oliver + Phtheirospermum Bunge (excluding Phtheirospermum japonicum [Thunberg] Kanitz); this supports reinstatement of Pseudobartsia and Xizangia. Although elements of Buchnereae‐Cymbarieae‐Orobancheae and Brandisieae‐Pterygielleae‐Rhinantheae showed incongruence among gene trees, the topology of the supermatrix tree was congruent with the majority of gene trees and functional‐group trees. Therefore, most plastid genes are evolving as a linkage group, allowing the supermatrix tree approach to yield internally consistent phylogenies for Orobanchaceae.
Perceived risk of COVID‐19 exposure and poor COVID‐19 prognosis impair sleep: The mediating and moderating roles of COVID‐19‐related anxiety and knowledge
Authors: Zerbini Giulia , Taflinger Shannon , Reicherts Philipp , Kunz Miriam , Sattler Sebastian 
Published: March 2022

AbstractThe ongoing COVID‐19 pandemic has been linked to increased levels of stress, depression, and anxiety in many people around the world. Therefore, identifying individuals at risk of psychosocial burden during this unprecedented crisis is essential for developing prevention measures and treatment options for mental health issues. To this aim, we investigated two risk groups: individuals at higher risk of exposure to the virus and individuals at higher risk of poor prognosis if they contract the virus. We conducted a survey (N = 4167) with a representative sample of the German population and assessed perceived risk of COVID‐19 exposure and poor prognosis if infected, COVID‐19‐related anxiety, problems with sleep and daytime functioning, as well as self‐reported knowledge about the disease. Results showed that perceived risk group membership was linked to increased problems with sleep and daytime functioning via elevated levels of COVID‐19‐related anxiety. This mediated effect was further moderated by self‐reported COVID‐19 knowledge, but only for individuals who rated themselves at higher risk of COVID‐19 exposure. Thus, knowledge buffered the negative effect of exposure risk on anxiety and ultimately on sleep in this risk group. Reaching individuals at increased risk of exposure with clear information about the disease, how to prevent infection, and treatment options could be an effective strategy to contain anxiety levels and promote good sleep, which is important for general well‐being.
L2 Classroom Input and L2 Positionally Sensitive Grammars: The Role of Information‐Seeking Question Sequences
Authors: HALL JOAN KELLY 
Published: March 2022

AbstractEvidence from usage‐based studies of second language (L2) acquisition reveals that a main source of L2 learners’ developing grammars is the L2 input to which learners are regularly exposed. What learners develop from their extended engagement in the sequences of actions comprising the input is not an acontextual system of grammatical units but rather thoroughly social, positionally sensitive grammars linked to the linguistic designs of the sequences. A growing body of research drawing on the theoretical framework and analytic methods of conversation analysis (CA) has identified the recurring interactional activities of L2 classrooms, which, for most adult L2 learners, are a major source of L2 input. Less examined are the linguistic designs of the interactional activities. This is the focus of the study reported here. Drawing on the shared theoretical and methodological framework of CA and interactional linguistics (IL), and building on previous work, the study examines the linguistic designs of information‐seeking sequences by which whole‐group instruction is accomplished. The focus is on teacher questions seeking factual information, the type of ‘knownness’ embodied in the questions, and both the actional types and linguistic designs of the student responses they engender. Findings show that while a great deal of opportunities for participation are made available to L2 learners in the information‐seeking sequences, the linguistic quality of the sequences is fairly limited in that the questions are designed primarily to engender one word or multiword phrases. These findings suggest that the possible pathways that learners’ developing L2 positionally sensitive grammars can take from their extended engagement in these sequences are also limited. For L2 teacher education, studies such as this one can enhance teachers’ understanding of the links between classroom input and learners’ developing L2 grammars, and the key role the teachers themselves play in designing the linguistic input of their L2 classroom contexts.
Unearthing the plant–microbe quid pro quo in root associations with beneficial fungi
Authors: Almario Juliana , Fabiańska Izabela , Saridis Georgios , Bucher Marcel 
Published: March 2022

SummaryMutualistic symbiotic associations between multicellular eukaryotes and their microbiota are driven by the exchange of nutrients in a quid pro quo manner. In the widespread arbuscular mycorrhizal (AM) symbiosis involving plant roots and Glomeromycotina fungi, the mycobiont is supplied with carbon through photosynthesis, which in return supplies the host plant with essential minerals such as phosphorus (P). Most terrestrial plants are largely dependent on AM fungi for nutrients, which raises the question of how plants that are unable to form a functional AM sustain their P nutrition. AM nonhost plants can form alternative, evolutionarily younger, mycorrhizal associations such as the ectomycorrhiza, ericoid and orchid mycorrhiza. However, it is unclear how plants such as the Brassicaceae species Arabidopsis thaliana, which do not form known mycorrhizal symbioses, have adapted to the loss of these essential mycorrhizal traits. Isotope tracing experiments with root‐colonizing fungi have revealed the existence of new ‘mycorrhizal‐like’ fungi capable of transferring nutrients such as nitrogen (N) and P to plants, including Brassicaceae. Here, we provide an overview of the biology of trophic relationships between roots and fungi and how these associations might support plant adaptation to climate change.
Sensitivity Analysis of Excess Mortality due to the COVID‐19 Pandemic
Authors: Nepomuceno Marília R. , Klimkin Ilya , Jdanov Dmitri A. , Alustiza‐Galarza Ainhoa , Shkolnikov Vladimir M. 
Published: March 2022

AbstractEstimating excess mortality is challenging. The metric depends on the expected mortality level, which can differ based on given choices, such as the method and the time series length used to estimate the baseline. However, these choices are often arbitrary, and are not subject to any sensitivity analysis. We bring to light the importance of carefully choosing the inputs and methods used to estimate excess mortality. Drawing on data from 26 countries, we investigate how sensitive excess mortality is to the choice of the mortality index, the number of years included in the reference period, the method, and the time unit of the death series. We employ two mortality indices, three reference periods, two data time units, and four methods for estimating the baseline. We show that excess mortality estimates can vary substantially when these factors are changed, and that the largest variations stem from the choice of the mortality index and the method. We also find that the magnitude of the variation in excess mortality is country‐specific, resulting in cross‐country rankings changes. Finally, based on our findings, we provide guidelines for estimating excess mortality.
Knowledge of Oneself and of Others: Aquinas, Wittgenstein and Rembrandt☆
Authors: Haldane John 
Published: March 2022

AbstractCurrent discussions of self‐knowledge focus on awareness of mental states, but a more ancient concern is with knowing one’s nature and with living well in light of that. Pursuit of this Socratic imperative has been associated with a tradition of self‐identification contributed to by Plato, Plotinus, Augustine and Descartes. Their ideas are distinguished and discussed, but another perspective is developed bringing together ideas from Aquinas and Wittgenstein: linking of the former’s understanding of propria and the latter’s account of criteria. The nation of natural signs is adverted to in relation to Reid suggesting a third component to this perspective.
Racial residential segregation, neighborhood disorder, and racial discrimination among black pregnant women
Authors: Dove‐Medows Emily , Misra Dawn P. , McCracken Lindsey , Giurgescu Carmen 
Published: March 2022

AbstractObjectiveTo compare and contrast pregnant, Black women's voices with quantitative measures of racial residential segregation, neighborhood disorder, and racial discrimination.Design and sampleUsing a convergent design for the parent study, surveys and qualitative interviews were completed by Black pregnant women (n = 27).MeasuresContent analysis was conducted and data were analyzed to assess for congruency or divergence for each concept related to structural racism (racial residential segregation, neighborhood disorder, and discrimination).ResultsNo single concept had 100% agreement across qualitative and quantitative approaches. Participants disclosed experiences during some interviews that were not captured by the surveys. The qualitative interviews offered a more detailed description of the concepts which along with the quantitative measures, provided insights about how participants perceived these mechanisms.DiscussionWhile important relationships about the mechanisms of structural racism and preterm birth can be examined using a single approach, using mixed methods can offer more insights about how those most impacted by preterm birth relate to these mechanisms. Future work will best add to the understanding of structural racism and preterm birth when study measures accurately reflect the experiences of the people who experience racism.
Interannual adjustments in stomatal and leaf morphological traits of European beech (Fagus sylvaticaL.) demonstrate its climate change acclimation potential
Authors: Petrik P. , Petek‐Petrik A. , Kurjak D. , Mukarram M. , Klein T. , Gömöry D. , Střelcová K. , Frýdl J. , Konôpková A. 
Published: March 2022

ABSTRACTThe current projections of climate change might exceed the ability of European forest trees to adapt to upcoming environmental conditions. However, stomatal and leaf morphological traits could greatly influence the acclimation potential of forest tree species subjected to global warming, including the single most important forestry species in Europe, European beech.We analysed stomatal (guard cell length, stomatal density and potential conductance index) and leaf (leaf area, leaf dry weight and leaf mass per area) morphological traits of ten provenances from two provenance trials with contrasting climates between 2016 and 2020. The impact of meteorological conditions of the current and preceding year on stomatal and leaf traits was tested by linear and quadratic regressions. Ecodistance was used to capture the impact of adaptation after the transfer of provenances to new environments.Interactions of trial–provenance and trial–year factors were significant for all measured traits. Guard cell length was lowest and stomatal density was highest across beech provenances in the driest year, 2018. Adaptation was also reflected in a significant relationship between aridity ecodistance and measured traits. Moreover, the meteorological conditions of the preceding year affected the interannual variability of stomatal and leaf traits more than the meteorological conditions of the spring of the current year, suggesting the existence of plant stress memory.High intraspecific variability of stomatal and leaf traits controlled by the interaction of adaptation, acclimation and plant memory suggests a high acclimation potential of European beech provenances under future conditions of global climate change.
An abrupt increase in foliage ABA levels on incipient leaf death occurs across vascular plants
Authors: McAdam S. A. M. , Kane C. N. , Mercado Reyes J. A. , Cardoso A. A. , Brodribb T. J. 
Published: March 2022

ABSTRACT

Forest mortality during drought has been attributed to hydraulic failure, which can be challenging to measure. A limited number of alternative proxies for incipient leaf death exist. Here we investigate whether a terminal increase in abscisic acid (ABA) levels in leaves occurs across vascular land plants and is an indicator of imminent leaf death.

For different species across vascular plants, we monitored ABA levels during lethal drought as well as leaf embolism resistance, across the canopy as leaves die following senescence, or when leaves are exposed to a heavy, lethal frost late in the growing season.

We observed a considerable increase in foliage ABA levels once leaves showed signs of incipient leaf death. This increase in ABA levels upon incipient leaf death, could be induced by embolism during drought, by freezing or as leaves age naturally, and was observed in species spanning the phylogeny of vascular land plants as well as in an ABA biosynthetic mutant plant.

A considerable increase in foliage ABA levels may act as an indicator of impending leaf death.
Seed biologists beware: Estimates of initial viability based on ungerminated seeds at the end of an experiment may be error‐prone
Authors: Lamont B. B. 
Published: March 2022

AbstractSeed viability is routinely measured on seeds that fail to germinate at the end of an experiment. Together with the number of germinants, this is used to estimate viability of the seeds at start of the experiment (i.e., initial viability) and provides the comparative basis on which germination success is determined. The literature and recent data on the germination requirements of Leucadendron species were examined to determine if there was any evidence for a treatment effect on viability of ungerminated seeds at the end of the experiment. The survey showed that sometimes (perhaps often, as the problem has yet to be recognized or reported) prolonged duration in the treatment, especially the control where little germination occurs, lead to loss of viability during the experiment. This resulted in underestimation of initial viability if that treatment was used. I caution against the routine use of end‐of‐trial germination and viability of ungerminated seeds as an estimate of initial viability in determining germination success of various treatments. I explore ways to deal with the problem but the preference is for estimates of initial viability to be undertaken on a separate sample of seeds concurrently with the experiment as this avoids the risk of seed death during the trial.
Quantifying habitat provisioning at macroalgal cultivation sites
Authors: Corrigan Sophie , Brown Andrew Ross , Ashton Ian G. C. , Smale Dan A. , Tyler Charles R. 
Published: March 2022

AbstractMacroalgal cultivation is expanding rapidly, and promises to contribute significantly towards future food and energy security, sustainable livelihoods, ecosystem services and habitat provisioning for a range of associated organisms globally. Habitat provisioning underpins biodiversity and ecosystem structure and functioning, supports many ecosystem services and has possible benefits to other marine industries, including enhancement of commercial fish stocks. In macroalgal cultivation, however, only recently has habitat provisioning started to be assessed at a local scale (within a farm's footprint) and with a range of different approaches. This review evaluates techniques used to quantify habitat provisioning in and around macroalgal cultivation sites, for species ranging from microorganisms to megafauna, and outlines recommendations to enable a more comprehensive ecological valuation of macroalgal cultivation in the future. The majority of information on biodiversity associated with macroalgal cultivation is associated with quantifying biofouling or pest organisms, rather than the contribution of colonising species to healthy ecosystem functioning. We suggest how better monitoring of macroalgal cultivation could enable an ecosystem approach to aquaculture (EAA) in the future. To achieve this, we highlight the need for standardised and robust methods for quantifying habitat provisioning that will enable assessment and monitoring of macroalgal cultivation sites of varying scales and within different regions and environmental settings. Increased evidence for the potential habitat value of macroalgal cultivation sites will help inform and shape marine legislation, licencing and certification for macroalgal farmers and potentially reduce marine user conflicts, helping the industry to continue to grow sustainably using EAA.
Exact uniformly most powerful postselection confidence distributions
Authors: Garcia‐Angulo Andrea C. , Claeskens Gerda 
Published: March 2022

AbstractA conditioning on the event of having selected one model from a set of possibly misspecified normal linear regression models leads to the construction of uniformly optimal conditional confidence distributions. They can be used for valid postselection inference. The constructed conditional confidence distributions are finite sample exact and encompass all information regarding the focus parameter in the selected model. This includes the construction of optimal postselection confidence intervals at all significance levels and uniformly most powerful hypothesis tests.
Focusing on different informant characteristics by situation: The dimensions of benevolence and competence in children's trust judgment
Authors: Miyoshi Mio , Sanefuji Wakako 
Published: March 2022

AbstractChildren use informant characteristics, such as competence or benevolence, in trust judgment. Previous studies have examined the characteristics that children prioritize in trust judgment, but findings have been conflicting. Since people possess and tap into each characteristic in their daily lives as the situation demands, children may adjust the characteristics that they choose to rely on depending on the specific situation rather than always prioritizing either of the characteristics. Thus, the present study investigated children's trust judgment in different situations requiring either competence or benevolence. Additionally, children's preference for competence or benevolence was examined to explore its effect on trust judgment. Results showed that children were more likely to select a competent rather than a benevolent person in situations requiring competence and vice versa in situations requiring benevolence. Children preferred the benevolent person rather than the competent person; such preference did not appear to affect their selection of informants across various situations. In summary, children might focus on the different characteristics of the informant in accordance with the situation. Their preference for benevolence might relate to a preference for prosociality. This, in turn, could relate to enhanced sensitivity to benevolence in early childhood.
Functional leaf anatomy of the invasive weed Solanum rostratum Dunal
Authors: Abu‐Nassar Jackline , Gal Shira , Shtein Ilana , Distelfeld Assaf , Matzrafi Maor 
Published: March 2022

AbstractSolanum rostratum Dunal is an aggressive invasive weed invading many countries worldwide. Our previous study indicated differences in herbicide response between young and mature plants. In this study, we explore the functional leaf surface anatomy of S. rostratum. Leaf area surface characterisation was performed by counting stomata, stellate and glandular trichomes on upper and lower leaf sides on scanning electron microscopy (SEM) micrographs. Total cuticular waxes were extracted and weighed in young and mature leaves. Young and mature leaves were tested for water permeability of the epidermal surfaces using the apoplastic tracer Calcofluor. S. rostratum plants were sprayed with luminous trace colour solution with and without 1% surfactant, droplet number and area were calculated. In young S. rostratum leaves, the leaf surface was densely covered with stellate trichomes on both leaf sides; however, in the mature leaf, stellate trichomes density became low, and prickle rigid increased in mature leaves. Wax amount was significantly higher in mature leaves, while number of stomata was higher in young leaves. Young leaves showed high leaf permeability, with the fluorescent dye permeating the leaf staining the vascular bundles. In young and mature leaves treated with a spraying solution with the addition of surfactant, low droplet number alongside higher coverage area was recorded. Our data highlight the notable differences in leaf epidermal structure of young versus mature plants. We conclude that differences in herbicide permeability, water loss and herbivory in young versus mature leaves of S. rostratum are governed by variation in leaf functional anatomy.
An Arbitrary Lagrangian–Eulerian Formulation of Two-Dimensional Viscoelastic Beams Based on the Consistent Corotational Method
Authors: Deng Lanfeng , Zhang Yahui , Chen Li-Qun 
Published: March 2022

Abstract
In this paper, an arbitrary Lagrangian–Eulerian (ALE) formulation based on the consistent corotational method is presented for the geometric nonlinear dynamic analysis of two-dimensional (2D) viscoelastic beams. In the ALE description, mesh nodes can be moved in some arbitrarily specified way, which is convenient for investigating problems with moving boundaries and loads. By introducing a corotational frame, the rigid-body motion of an element can be removed. Then, the pure deformation and the deformation rate of the element can be measured in the local frame. This method can avoid rigid-body motion damping. In addition, the elastic force vector, the inertia force vector, and the internal damping force vector are derived with the same shape functions to ensure the consistency and independence of the element. Therefore, different assumptions can be made to describe the local deformation of the element. In this paper, the interdependent interpolation element (IIE) and the Kelvin–Voigt model are introduced in the local frame to consider the shear deformation, rotary inertia, and viscoelasticity. Moreover, the presented method is capable of considering the arbitrary curved initial geometry of a beam. Numerical examples show that internal damping dampens only the pure elastic deformation of the beam but does not dampen the rigid-body motion. Three dynamic problems of a beam with a moving boundary or subjected to a moving load are investigated numerically by the presented formulation and the commercial software ansys to verify the validity, versatility, and computational efficiency of the presented formulation.