bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using DNA metabarcoding as a novel approach for analysis of platypus diet
Authors: Hawke Tahneal , Bino Gilad , Shackleton Michael E. , Ross Alexandra K. , Kingsford Richard T. 
Published: February 2022

AbstractPlatypuses (Ornithorhynchus anatinus) forage for macroinvertebrate prey exclusively in freshwater habitats. Because food material in their faeces is well digested and mostly unidentifiable, previous dietary studies have relied on cheek pouch assessments and stable isotope analysis. Given DNA metabarcoding can identify species composition from only fragments of genetic material, we investigated its effectiveness in analysing the diet of platypuses, and to assess variation across seasons and sexes. Of the 18 orders and 60 families identified, Ephemeroptera and Diptera were the most prevalent orders, detected in 100% of samples, followed by Trichoptera, Pulmonata, and Odonata (86.21% of samples). Caenidae and Chironomidae were the most common families. Diptera had a high average DNA read, suggesting it is an important dietary component that may have been underestimated in previous studies. We found no variation in diet between sexes and only minimal changes between seasons. DNA metabarcoding proved to be a highly useful tool for assessing platypus diet, improving prey identification compared to cheek pouch analysis, which can underestimate soft-bodied organisms, and stable isotope analysis which cannot distinguish all taxa isotopically. This will be a useful tool for investigating how platypus prey diversity is impacted by habitat degradation as a result of anthropogenic stressors.
Beyond Dunbar circles: a continuous description of social relationships and resource allocation
Authors: Tamarit Ignacio , Sánchez Angel , Cuesta José A. 
Published: February 2022

AbstractWe discuss the structure of human relationship patterns in terms of a new formalism that allows to study resource allocation problems where the cost of the resource may take continuous values. This is in contrast with the main focus of previous studies where relationships were classified in a few, discrete layers (known as Dunbar’s circles) with the cost being the same within each layer. We show that with our continuum approach we can identify a parameter $$\eta $$
η
that is the equivalent of the ratio of relationships between adjacent circles in the discrete case, with a value $$\eta \sim 6$$

η
∼
6

. We confirm this prediction using three different datasets coming from phone records, face-to-face contacts, and interactions in Facebook. As the sample size increases, the distributions of estimated parameters smooth around the predicted value of $$\eta $$
η
. The existence of a characteristic value of the parameter at the population level indicates that the model is capturing a seemingly universal feature on how humans manage relationships. Our analyses also confirm earlier results showing the existence of social signatures arising from having to allocate finite resources into different relationships, and that the structure of online personal networks mirrors those in the off-line world.
Diffuse optical reconstructions of functional near infrared spectroscopy data using maximum entropy on the mean
Authors: Cai Zhengchen , Machado Alexis , Chowdhury Rasheda Arman , Spilkin Amanda , Vincent Thomas , Aydin Ümit , Pellegrino Giovanni , Lina Jean-Marc , Grova Christophe 
Published: February 2022

AbstractFunctional near-infrared spectroscopy (fNIRS) measures the hemoglobin concentration changes associated with neuronal activity. Diffuse optical tomography (DOT) consists of reconstructing the optical density changes measured from scalp channels to the oxy-/deoxy-hemoglobin concentration changes within the cortical regions. In the present study, we adapted a nonlinear source localization method developed and validated in the context of Electro- and Magneto-Encephalography (EEG/MEG): the Maximum Entropy on the Mean (MEM), to solve the inverse problem of DOT reconstruction. We first introduced depth weighting strategy within the MEM framework for DOT reconstruction to avoid biasing the reconstruction results of DOT towards superficial regions. We also proposed a new initialization of the MEM model improving the temporal accuracy of the original MEM framework. To evaluate MEM performance and compare with widely used depth weighted Minimum Norm Estimate (MNE) inverse solution, we applied a realistic simulation scheme which contained 4000 simulations generated by 250 different seeds at different locations and 4 spatial extents ranging from 3 to 40$$\text {cm}^2$$

cm
2

along the cortical surface. Our results showed that overall MEM provided more accurate DOT reconstructions than MNE. Moreover, we found that MEM was remained particularly robust in low signal-to-noise ratio (SNR) conditions. The proposed method was further illustrated by comparing to functional Magnetic Resonance Imaging (fMRI) activation maps, on real data involving finger tapping tasks with two different montages. The results showed that MEM provided more accurate HbO and HbR reconstructions in spatial agreement with the main fMRI cluster, when compared to MNE.
Occurrence of plasmid-mediated quinolone resistance genes in Pseudomonas aeruginosa strains isolated from clinical specimens in southwest Iran: a multicentral study
Authors: Saki Morteza , Farajzadeh Sheikh Ahmad , Seyed-Mohammadi Sakineh , Asareh Zadegan Dezfuli Aram , Shahin Mojtaba , Tabasi Maryam , Veisi Hojat , Keshavarzi Raziyeh , Khani Parisa 
Published: February 2022

AbstractThis study aimed to assess the presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA, and aac(6′)-Ib-cr determinants as well as quinolone resistance pattern of clinical isolates of P. aeruginosa in Ahvaz, southwest Iran. A total of 185 clinical isolates of P. aeruginosa were collected from 5 university-affiliated hospitals in Ahvaz, southwest Iran. The disk diffusion method was applied to assess the quinolone resistance pattern. The presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA, and aac(6′)-Ib-cr genes was investigated by the polymerase chain reaction (PCR) method. Overall, 120 (64.9%) isolates were non-susceptible to quinolones. The most and the less quinolone resistance rates were observed against ciprofloxacin (59.4%) and ofloxacin (45.9%), respectively. The prevalence rates of qnr genes were as follows: qnrA (25.8%), qnrB (29.2%), and qnrS (20.8%). The qnrB gene was the most common type of qnr genes. The qnr genes were occurred in 37.5% (n = 45/120) of quinolne-resistant isolates, simultaneously. The qnrC, qnrD, qepA, and aac(6′)-Ib-cr genes were not recognized in any isolates. In conclusion, the ofloxacin was the most effective quinolone. This study was the first to shed light on the prevalence of PMQR genes among P. aeruginosa isolates in southwest Iran.
Network control by a constrained external agent as a continuous optimization problem
Authors: Nys Jannes , van den Heuvel Milan , Schoors Koen , Merlevede Bruno 
Published: February 2022

AbstractSocial science studies dealing with control in networks typically resort to heuristics or solely describing the control distribution. Optimal policies, however, require interventions that optimize control over a socioeconomic network subject to real-world constraints. We integrate optimisation tools from deep-learning with network science into a framework that is able to optimize such interventions in real-world networks. We demonstrate the framework in the context of corporate control, where it allows to characterize the vulnerability of strategically important corporate networks to sensitive takeovers, an important contemporaneous policy challenge. The framework produces insights that are relevant for governing real-world socioeconomic networks, and opens up new research avenues for improving our understanding and control of such complex systems.
Comparison of mental health outcomes in seropositive and seronegative adolescents during the COVID19 pandemic
Authors: Blankenburg Judith , Wekenborg Magdalena K. , Reichert Jörg , Kirsten Carolin , Kahre Elisabeth , Haag Luise , Schumm Leonie , Czyborra Paula , Berner Reinhard , Armann Jakob P. 
Published: February 2022

AbstractPost-COVID19 complications such as pediatric inflammatory multisystem syndrome (PIMS) and Long-COVID19 move increasingly into focus, potentially causing more harm in young adolescents than the acute infection. To better understand the symptoms of long-term mental health outcomes in adolescents and distinguish infection-associated symptoms from pandemic-associated symptoms, we conducted a 12 question Long-COVID19 survey. Using this survey, we compared the responses on neurocognitive, general pain and mood symptoms from seropositive and seronegative adolescents in a cross-sectional study design. Since May 2020, students grade 8–12 in fourteen secondary schools in Eastern Saxony were enrolled in the SchoolCovid19 study. Serostatus was assessed regularly in all participants. In March/April 2021, 1560 students with a median age of 15 years participated at the regular study visit after re-opening of the schools in mid-March and responded to our Long-COVID19 survey as part of this visit. 1365 (88%) students were seronegative, 188 (12%) were seropositive. Each symptom asked in the Long-COVID19 survey was present in at least 35% of the students within the last seven days before the survey. With the exception of seropositive students being less sad, there was no significant difference comparing the reported symptoms between seropositive students and seronegative students. The lack of differences comparing the reported symptoms between seropositive and seronegative students suggests that Long-COVID19 might be less common than previously thought and emphasizes on the impact of pandemic-associated symptoms regarding the well-being and mental health of young adolescents.Clinical Trial Registration: SchoolCoviDD19: Prospektive Erfassung der SARS-CoV-2 Seropositivität bei Schulkindern nach Ende der unterrichtsfreien Zeit aufgrund der Corona-Schutz-Verordnung (COVID-19), DRKS00022455, https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00022455
Derivation of healthy hepatocyte-like cells from a female patient with ornithine transcarbamylase deficiency through X-inactivation selection
Authors: Santamaria Ramon , Ballester Maria , Garcia-Llorens Guillem , Martinez Francisco , Blazquez Marina , Ribes-Koninckx Carmen , Castell Jose V. , Wuestefeld Torsten , Bort Roque 
Published: February 2022

AbstractAutologous cell replacement therapy for inherited metabolic disorders requires the correction of the underlying genetic mutation in patient’s cells. An unexplored alternative for females affected from X-linked diseases is the clonal selection of cells randomly silencing the X-chromosome containing the mutant allele, without in vivo or ex vivo genome editing. In this report, we have isolated dermal fibroblasts from a female patient affected of ornithine transcarbamylase deficiency and obtained clones based on inactivation status of either maternally or paternally inherited X chromosome, followed by differentiation to hepatocytes. Hepatocyte-like cells derived from these clones display indistinct features characteristic of hepatocytes, but express either the mutant or wild type OTC allele depending on X-inactivation pattern. When clonally derived hepatocyte-like cells were transplanted into FRG® KO mice, they were able to colonize the liver and recapitulate OTC-dependent phenotype conditioned by X-chromosome inactivation pattern. This approach opens new strategies for cell therapy of X-linked metabolic diseases and experimental in vitro models for drug development for such diseases.
ILF2 enhances the DNA cytosine deaminase activity of tumor mutator APOBEC3B in multiple myeloma cells
Authors: Kazuma Yasuhiro , Shirakawa Kotaro , Tashiro Yusuke , Yamazaki Hiroyuki , Nomura Ryosuke , Horisawa Yoshihito , Takeuchi Suguru , Stanford Emani , Konishi Yoshinobu , Matsui Hiroyuki , Matsumoto Tadahiko , Tanabe Fumiko , Morishita Ryo , Ito Shinji , Takaori-Kondo Akifumi 
Published: February 2022

AbstractDNA cytosine deaminase APOBEC3B (A3B) is an endogenous source of mutations in many human cancers, including multiple myeloma. A3B proteins form catalytically inactive high molecular mass (HMM) complexes in nuclei, however, the regulatory mechanisms of A3B deaminase activity in HMM complexes are still unclear. Here, we performed mass spectrometry analysis of A3B-interacting proteins from nuclear extracts of myeloma cell lines and identified 30 putative interacting proteins. These proteins are involved in RNA metabolism, including RNA binding, mRNA splicing, translation, and regulation of gene expression. Except for SAFB, these proteins interact with A3B in an RNA-dependent manner. Most of these interacting proteins are detected in A3B HMM complexes by density gradient sedimentation assays. We focused on two interacting proteins, ILF2 and SAFB. We found that overexpressed ILF2 enhanced the deaminase activity of A3B by 30%, while SAFB did not. Additionally, siRNA-mediated knockdown of ILF2 suppressed A3B deaminase activity by 30% in HEK293T cell lysates. Based on these findings, we conclude that ILF2 can interact with A3B and enhance its deaminase activity in HMM complexes.
Negative impact of the hypopnea index or duration increase after a non-frame work surgery in patients with very severe obstructive sleep apnea
Authors: Huang Ethan I. , Huang Shu-Yi , Lin Yu-Ching , Lin Chieh-Mo , Lin Chin-Kuo , Huang Ying-Chih , Hsu Chia-Yu , Su Jian-An 
Published: February 2022

AbstractA non-framework surgery could change the postoperative components of breathing disturbances and increase the frequency or duration of hypopnea in patients with very severe obstructive sleep apnea (OSA). Either an increase of hypopnea index, which increases apnea–hypopnea index (AHI), or an increase of its duration raises the concern of worsening the oxygen desaturation and so morbidity and mortality associated with OSA. It is unclear how the oxygen saturation would change in those having increased frequency or duration of hypopneas after the surgery. Here in 17 patients with AHI ≥ 60 events/h, having increased frequency or duration of hypopneas after the non-framework surgery, the results show that the surgery improved oxygen saturation by reducing obstructive-apnea index (36.1 events/h) and duration (8.6 s/event), despite it increased hypopnea index (16.8 events/h) and duration (9.8 s/event). The surgery improved the average of the mean oxyhemoglobin saturation of pulse oximetry (SpO2) by 2.8% (toward a ceiling mean of 94.3%), mean minimal SpO2 by 7.5%, and mean desaturation by 5%. The results suggest sufficient apnea reduction and shift from apnea to hypopnea may mask the negative impact of the increase of hypopnea index or duration and improve postoperative mean SpO2, minimal SpO2, and mean desaturation.
Interaction with the future self in virtual reality reduces self-defeating behavior in a sample of convicted offenders
Authors: van Gelder Jean-Louis , Cornet Liza J. M. , Zwalua Natascha P. , Mertens Esther C. A. , van der Schalk Job 
Published: February 2022

AbstractIn this study, we test an intervention in which convicted offenders interacted with an age-progressed avatar representing their future selves in virtual reality. During the interaction, they reflected on their current lifestyle, alternating between the perspective of their present self and that of their future self. We hypothesized that this embodied experience would increase their ability to imagine themselves in the future and reduce their engagement in self-defeating behavior, as measured with a self-report survey. In line with expectations, results indicated that the interaction increased vividness of the future self compared to baseline and reduced self-defeating behavior, including alcohol use and overspending, one week later. In addition, increases in vividness were associated with a reduction in self-defeating behavior over and above other concepts relating to the future self, including connectedness, similarity, and valence. The results are based on a small sample and should therefore be considered as indicative of the possibilities of our virtual reality paradigm as an intervention tool to reduce self-defeating behavior.
Size and albedo of the largest detected Oort-cloud object: Comet C/2014 UN271 (Bernardinelli-Bernstein)
Authors: Lellouch E. , Moreno R. , Bockelée-Morvan D. , Biver N. , Santos-Sanz P. 
Published: February 2022

Context. The recently announced Oort-cloud comet C/2014 UN271 (Bernardinelli-Bernstein) is remarkable in at least three respects: (i) it was discovered inbound as far as ∼29 au from the Sun (with prediscovery images up to ∼34 au); (ii) it already showed cometary activity at almost 24 au; and (iii) its nuclear magnitude (Hr ∼ 8.0) indicates an exceptionally large object. Detection of gases is expected in the upcoming years as the comet heads toward a perihelion of ∼11 au in 2031.
Aims. The goal is to determine the object’s diameter and albedo from thermal measurements.
Methods. We used ALMA in extended configuration (resolution ∼0.064″) to measure the 1287 μm (233 GHz) continuum flux of the comet. Observations were performed on August 8, 2021, at a 20.0 au distance from the Sun. The high spatial resolution was chosen in order to filter out any dust contribution. We also used a recently published Afρ value to estimate the dust production rate and the expected dust thermal signal for various assumptions on particle size distribution.
Results. We detected the thermal emission of the object at ∼10σ, with a flux of 0.128 ± 0.012 mJy. Based on observational constraints and our theoretical estimates of the dust contribution, the entirety of the measured flux can be attributed to the nucleus. From NEATM modeling combined with the Hr magnitude, we determine a surface-equivalent diameter of 137 ± 17 km and a red geometric albedo of 5.3 ± 1.2%. This confirms that C/2014 UN271 is by far the largest Oort-cloud object ever found (almost twice as large as comet C/1995 O1 Hale-Bopp) and, except for the Centaur 95P/Chiron, which shows outburst-like activity, the largest known comet in the Solar System. On the other hand, the C/2014 UN271 albedo is typical of comets, adding credence to a “universal” comet nucleus albedo.
Conclusions. With its distant perihelion and uniquely large size, C/2014 UN271 (Bernardinelli-Bernstein) is the prominent archetype of distant comets whose activity is driven by hypervolatiles. Monitoring of dust and gas emission as the comet approaches and passes perihelion will permit its activity time pattern to be studied and compared to the distant (outbound) activity of Hale-Bopp. Post-perihelion thermal measurements will permit the study of possible albedo changes, such as a surface brightening compared to pre-perihelion, as was observed for Hale-Bopp.
A novel highly dispersed tetra-metal nano heterogeneous ozone catalyst derived from microbial adsorption and in situ pyrolysis
Authors: Xie Jianping , Fan Wei , Cui Hao , Feng Ningning , Wang Guozhen , Wang Hui , Liu Xinxing , Qiu Guanzhou , Wu Haiyan 
Published: February 2022

Abstract
In recent years, the pyrolysis of microbial biomasses that adsorb various metal ions has enabled the preparation of carbon-based polymetallic nanomaterials with excellent electrocatalytic and electrical energy storage properties. However, the preparation of ozone catalysts by this technique and the corresponding catalytic oxidation mechanism are still unclear. In this study, an Escherichia coli strain (BL21) was used for tetra-metal (Cu, Fe, Mn and Al) absorption and the obtained microbial biomass was pyrolyzed under the protection of a nitrogen flow at 700 °C and activated at 900 °C to prepare a microbial-char-based tetra-metal ozone catalyst (MCOC). This was used to degrade phenol and coking wastewater and exhibited a strong catalytic capability for coking wastewater, whose chemical oxygen demand removal efficiency of 70.86% is 16.7% higher than that of pure ozone and 14.67%, 7.21% and 3.58% higher than that of three commercial catalysts, respectively. It also improved the efficiency of ozonation for phenol by 33%. The MCOC was characterized by x-ray diffraction, x-ray photoelectron spectroscopy, scanning electron microscopy–energy-dispersive spectroscopy, transmission electron microscopy and other methods. The results demonstrated that the spherical metal nanoparticles had sizes ranging from 3 nm to 7 nm and that crystals of Fe2O3 and Fe3P were observed. The study showed that the MCOC promoted the production of more hydroxyl radicals and superoxides from ozone, which attack organics. The oxygen vacancies of the catalyst were also investigated. It was proved that the Lewis acid sites on the surface of metal oxides are the active centers of ozone decomposition. Therefore, this work provides a new method for the synthesis of multi-metal nanocomposites and expands the application of biosynthetic nanomaterials.
Electronic structure and optical properties of non-metallic modified graphene: a first-principles study
Authors: Huang Jing-tao , Liu Yong , Lai Zhong-hong , Hu Jin , Zhou Fei , Zhu Jing-chuan 
Published: February 2022

Abstract
In this paper, the electronic structure and stability of the intrinsic, B-, N-, Si-, S-doped graphene are studied based on first-principles calculations of density functional theory. Firstly, the intrinsic, B-, N-, Si-, S-doped graphene structures are optimized, and then the forming energy, band structure, density of states, differential charge density are analyzed and calculated. The results show that B- and Si-doped systems are p-type doping, while N is n-type doping. By comparing the forming energy, it is found that N atoms are more easily doped in graphene. In addition, for B-, N-, Si-doped systems, it is found that the doping atoms will open the band gap, leading to a great change in the band structure of the doping system. Finally, we systematically study the optical properties of the different configurations. By comparison, it is found that the order of light sensitivity in the visible region is as follows: S-doped> Si-doped> pure > B-doped > N-doped. Our results will provide theoretical guidance for the stability and electronic structure of non-metallic doped graphene.
Dynamics of mixed lump-soliton for an extended (2+1)-dimensional asymmetric Nizhnik–Novikov–Veselov equation
Authors: Shi Kai-Zhong , Shen Shou-Feng , Ren Bo , Wang Wan-Li 
Published: February 2022

Abstract
A new (2+1)-dimensional higher-order extended asymmetric Nizhnik–Novikov–Veselov (eANNV) equation is proposed by introducing the additional bilinear terms to the usual ANNV equation. Based on the independent transformation, the bilinear form of the eANNV equation is constructed. The lump wave is guaranteed by introducing a positive constant term in the quadratic function. Meanwhile, different class solutions of the eANNV equation are obtained by mixing the quadratic function with the exponential functions. For the interaction between the lump wave and one-soliton, the energy of the lump wave and one-soliton can transfer to each other at different times. The interaction between a lump and two-soliton can be obtained only by eliminating the sixth-order bilinear term. The dynamics of these solutions are illustrated by selecting the specific parameters in three-dimensional, contour and density plots.