bluuebunny/crossref_metadata_2025_split huggingface dataset:
Přehledová studie výzkumů předškolního vzdělávání v České republice v letech 2011–2020
Authors: Syslová Zora , Najvarová Veronika 
Published: January 2022

Studie přináší přehled témat a empirických poznatků z výzkumů provedených v oblasti předškolního vzdělávání v České republice v letech 2011–2020. Navazuje na přehledovou studii výzkumů předškolního vzdělávání v letech 2000–2010. Studie vychází z analýzy výzkumných statí, které byly ve sledovaném období publikovány v odborných časopisech a relevantních sbornících. Předkládaná studie odpovídá na výzkumné otázky: (1) Jaká témata byla ve výzkumech řešena a jaké cíle byly sledovány? (2) Jaké metody byly využívány a na jakých souborech byly studie realizovány? (3) Jaká podstatná zjištění a závěry z těchto studií vyplynuly? Na základě analýzy těchto studií dospěly autorky ke zjištěním, že během sledovaného období byl přesunut publikační důraz do odborných časopisů, nicméně nedošlo k výrazné změně v oblasti zpracovávaných témat. V oblasti metodologie provedených výzkumů je možné sledovat posun ke kvalitativní až smíšené metodologii.
Network pharmacology study of Citrus reticulata and Pinellia ternata in the treatment of non-small cell lung cancer
Authors: Li Changyang , Lu Hongxiu , Jiang Xianghu , Guo Xuefeng , Zhong Hua , Li Huijie 
Published: January 2022

It has been recognized that Citrus reticulata and Pinellia ternata have a good therapeutic effect on NSCLC. However, the potential mechanism of C. reticulata and P. ternata in the treatment of NSCLC based on network pharmacology analysis is not clear. The “Drug-Component-Target-Disease” network was constructed by Cytoscape, and the protein interaction (PPI) network was constructed by STRING. Our study indicated that 18 active ingredients of C. reticulata and P. Ternata were screened from the TCMSP database, and 56 target genes of C. reticulata and P. Ternata for the treatment of NSCLC were identified, and we constructed the “Drug-Component-Target-Disease” network. In this study, we screened 56 PPI core genes to establish a PPI network. We concluded that the network pharmacology mechanism of the effect of C. reticulata and P. Ternata on NSCLC may be closely related to the protein expressed by TP53, ESR1, FOS, NCOA3 and MAPK8, and these may play the therapeutic roles by regulating the IL-17 signaling pathway, antigen processing and presentation, microRNAs in cancer and endocrine resistance.
Predictive factors of extracorporeal shock wave lithotripsy failure in the treatment of patients with ureterolithiasis
Authors: Vozianov O. S. , Vozianov S. O. 
Published: January 2022

Efficacy of different approaches to the treatment in patients with ureteral calculi and predictive factors for the success are the objects of contemporary studies. However, only few on them studied the duration factor of typical ureterolithiasis symptoms and changes of the ureter wall as predictive factors of extracorporeal shock wave lithotripsy (ESWL) failure in patients with ureteral stones.
The aim of the study was to determine the peculiarities of histological changes in the ureter wall as a result of calculus presence and to evaluate the predictive factors of ESWL failure in patients with ureterolithiasis.
Materials and methods. An analysis of the treatment by ESWL in 662 patients with ureterolithiasis was performed. The patients were divided into two groups: Group 1, n = 629 (95.0 %) individuals with effective ESWL and Group 2, n = 33 (5.0 %) patients with ESWL failure when calculi were not eliminated. Stones were found in 378 (57.0 %) patients in the upper third of the ureter, in 50 (7.6 %) – in the middle and in 234 (35.4 %) – in the lower third. For determining the predictors of ESWL failure, the Spearman’s Rank Correlation Coefficient was used.
Results. The overall ESWL efficacy in our study was 95.0 %. The strong inverse correlation was found between the ESWL success and duration of symptoms (r = -0.92) and stone size (r = -0.68). Stone localization in the ureter did not influence the ESWL outcomes (r = 0.27).
Conclusions. Increasing of the period from the manifestation of primary clinical symptoms to the implementation of ESWL reduces the treatment efficacy. Patients with ureteral stones more than 15 mm have lower stone-free rates after ESWL.
Gender peculiarities of clinical and instrumental indexes in patients with acute Q-wave myocardial infarction after primary coronary intervention
Authors: Savchenko Yu. V. , Kyselov S. M. 
Published: January 2022

The aim: to determine gender differences in the course of acute Q-wave myocardial infarction in patients after primary coronary intervention.
Materials and methods. Results of clinical and instrumental examination of 58 patients with acute Q-wave myocardial infarction were analyzed. Patients were divided into 2 groups according to gender: group 1 (n = 33) – men, mean age 61.0 (55.0; 69.0) years, group 2 (n = 25) – women, mean age 69.0 (63.0; 78.0) years. All the patients underwent coronary angiography followed by angioplasty and stenting of the infarct-dependent coronary artery.
Results. In men, the number of leukocytes was significantly higher (by 24.7 %, P = 0.02) and the plasma level of low-density lipoproteins was significantly lower (by 30.9 %, P = 0.007), there was a tendency towards a higher concentration of creatine kinase-MB (by 41.0 %, P = 0.11) at the time of hospital admission as compared to women. Women showed significantly increased thickness of the posterior wall of the left ventricle (LV) (by 7.31 %, P = 0.02) and a tendency towards the prevalence of the interventricular septum wall thickness (by 7.46 %, P = 0.1). The LV end-diastolic and end-systolic dimensions tended to prevail in men (by 2.41 %, P = 0.47 and by 7.81 %, P = 0.19, respectively). Men were found to have a lower peak A rate (by 23.37 %, P = 0.007), a higher E/A ratio (by 52.27 %, P = 0.009), LV diastolic dysfunction (DD) occurred less frequently (χ2 = 2.89, P = 0.08), and there was a tendency to develop eccentric LV hypertrophy (χ2 = 2.39, P = 0.12) and type 2 LV DD (χ2 = 3.82, P = 0.07). Type 1 DD was more common in women (χ2 = 5.71, P = 0.02). In men, there was a tendency to the formation of zones of LV akinesia (χ2 = 2.5, P = 0.09) and decreased LV ejection fraction (χ2 = 1.57, P = 0.31).
Conclusions. In the acute period of Q-wave myocardial infarction after revascularization, in men, unlike in women, systolic dysfunction is developed more often. It is accompanied by acute left ventricular failure, eccentric left ventricular hypertrophy and type 2 diastolic dysfunction formation as well as signs of systemic inflammation. In women, in the acute period of Q-wave myocardial infarction after revascularization, concentric left ventricular remodeling occurs more frequently and is accompanied by type 1 left ventricular diastolic dysfunction.
The role of the macrophage polarization type in the pathogenesis of endometrioid disease
Authors: Orlova Y. А. , Gromova A. М. , Akimov O. Ye. , Ketova O. М. 
Published: January 2022

Endometriosis today occupies one of the leading places in the structure of general gynecological pathology. Theories of the onset and progression of this disease are controversial. One of the most widespread theories is the assumption that endometriosis is a disease of macrophages. The question of which the macrophage phenotype, M1 or M2, is the leading one, however, remains controversial.
The aim. To determine the type of macrophage polarization (M1/M2) and the quantitative activity of their marker enzymes (iNos/Arg1) in the endometrium and peritoneal fluid in endometrioid disease.
Materials and methods. The total number of reproductive age (30.95±6.49) women enrolled in the study was 80. The main group consisted of 50 women with endometrioid disease. The control group included 30 women without signs of endometrioid disease. Women from the main group (n=24) and the control group (n=27) underwent endometrial sampling using an intrauterine Pipelle catheter in the first phase of the menstrual cycle before a surgery. During laparoscopic or laparotomy approaches, peritoneal fluid was taken (in the main group n=24, in the control group n=28). The type of macrophage polarization (M1 or M2) was determined based on the ratio of marker enzymes (Arg1, iNos) activity in each patient using a spectrophotometric method in the endometrium and peritoneal fluid. The polarization to the M1 phenotype was determined at iNos>Arg1, and at Arg1>iNos– the polarization to the M2 phenotype.
Results. As a result of the study, it was revealed that in women with endometrioid disease, pelvic adhesions were much more common, 84.0% versus 46.7% in women without it, and especially 3 and 4 degree of severity (P<0.05). When assessing the type of macrophages in the peritoneal fluid, a significantly greater number of the main group women had the M2 phenotype of macrophage polarization compared to the control group (58.3% versus 28.6%, P=0.03). It was the macrophage polarization to the M2 phenotype that influenced the severity of endometrioid disease, especially the 4 degree of severity. The mean values of the iNOS activity in the main group women, both in the peritoneal fluid and in the endometrium, significantly differed from those in the control group patients (by 1.73 and 1.77 times, respectively).
Conclusions. Thus, we can conclude that endometriosis is a disease, the development and progression of which is induced by the M2 phenotype of macrophages. Considering the increase in the mean levels of iNOS activity both in the peritoneal fluid and in the endometrium, it can be concluded that iNOS influences the pathogenesis of endometrioid disease.
Biliary reflux after laparoscopic modified antireflux monoanastomotic gastric bypass surgery
Authors: Nikolaiev M. V. 
Published: January 2022

Aim. Determination of biliary reflux in patients with morbid obesity after a single-anastomotic gastric bypass operation performed in the clinic's modification and according to the classical Roux-en-Y gastric bypass technique. Development of diagnostic criteria for biliary reflux
Materials and methods. The results of treatment of 36 patients with morbid obesity who underwent bypass surgery were studied. The patients are divided into two groups. Clinically modified laparoscopic monoanastomotic gastric bypass surgery was performed in 25 patients (main group). The control group included 11 patients after the standard technique of Roux-en-Y laparoscopic gastric bypass. Inclusion criteria were: persons of both sexes aged 18-60 years with morbid obesity and a body mass index of 40 or more, as well as 35 or more in the presence of comorbid diseases (type 2 diabetes mellitus, arterial hypertension, dyslipidemia, sleep apnea syndrome). In both groups, impedance pH measurements were performed in the postoperative period. Statistical processing was performed using the Statistica 13.0 software package using parametric and nonparametric statistical methods.
Results: Clinical manifestations of the enterogastric biliary reflex were found in both groups; in addition, clinical manifestations of the corresponding symptoms were noted by patients of both groups. The analysis of daily pH-metry in the studied groups showed that in the esophagus the time with pH <4 in the main group 2.83 (1.55; 3.95)% 3.00 (2.30; 3.50)% in the control group, time with pH 4-6.9 (physiological for the esophagus) 92.40 (90.65; 94.20)% and 94.10 (89.80; 95.50)%, respectively, time with pH> 7 (weak alkaline) was 4.80 (3.45; 5.85)% and in the control group 2.90 (1.20; 7.20)%. There was no statistically significant difference in these indicators (p> 0.05). Conclusions. Monoanastomotic gastric bypass surgery in the modification of the clinic has the same positive properties as the Rouen-Wye technique, allowing you to avoid the risks associated with possible pathological reflux of bile into the esophagus. Patients who have undergone mini-gastric bypass surgery require a thorough examination with fibrogastroscopy in combination with pH impedance measurement at least 1 time per year in order to determine biliary reflux and morphological changes both in the lumen of the esophagus and in the stomach stump. The level of quality of life of patients after surgery in the control group and the main group does not significantly differ, as evidenced by the results of the questionnaire survey using the GERG Q questionnaire, which indicates the effectiveness of the methodology of laparoscopic monoanastomotic gastric bypass surgery modified in the clinic. Key words: morbid obesity, gastric bypass surgery, surgical treatment, mini-gastric bypass, biliary reflux.
Study of the efficacy of antimicrobial preservatives in justifying the composition of a dermatological gel with a phytocomplex
Authors: Myrhorod V. S. , Bashura O. H. , Strilets O. P. , Bobro S. H. , Strelnykov L. S. 
Published: January 2022

The microbiological stability of medicinal products always requires close attention during the pharmaceutical development phase, as microbial contamination can pose a threat to both the health of the patient and the stability of the medicinal product. The level of microbial contamination can be controlled by monitoring the quality of raw materials, compliance with appropriate sanitation of production facilities and equipment, the use of scientifically justified preservatives in the drug.
The aim of the work is to substantiate the use of a preservative and its concentration in the composition of the developed gel with phytocomplex.
Materials and methods. The objects of the study were gel samples with the addition of a preservative: Euxyl 9010K (90 % phenoxyethanol, 10 % ethylhexylglycerol), methyl parahydroxybenzoate (E218), sorbic acid, potassium sorbate, benzoic acid. Concentrations of antimicrobial substances used corresponded to their average value from the range of used concentrations. The research has used the method of evaluating the effectiveness of antimicrobial preservatives, given in SPU 2.0.
Results. Experimental studies using preservatives Euxyl 9010K 0.60 %, methyl parahydroxybenzoate 0.25 %, sorbic acid 0.10 %, potassium sorbate 0.25 %, benzoic acid 0.15 % in the samples of the developed gel with phytocomplex had shown that the obtained results for all samples fully meet the requirements of SPU in terms of “antimicrobial efficacy of preservatives” for topical drugs. According to the results of the first stage of research, it had been found that the greatest antimicrobial efficacy was shown by a sample with the preservative Euxyl 9010K. The subject of the second stage of research was the substantiation of the concentration of Euxyl 9010K (0.45 %, 0.60 %, and 0.75 %) based on the results of which it had been established that the gel samples with concentrations of Euxyl 9010K 0.60 % and 0.75 % met the requirements of SPU on the indicator of “antimicrobial efficacy of preservatives” for topical medicinal products. The sample with a concentration of Euxyl 9010K 0.45 % also met these requirements, but the logarithm of the reduction in the number of viable cells of Pseudomonas aeruginosa bacteria after 2 days of storage is 2.00, which was the limit value according to the requirements of SPU.
Conclusions. The expediency of using Euxyl 9010K (90 % phenoxyethanol, 10 % ethylhexylglycerol) at a concentration of 0.60 % as a preservative had been experimentally substantiated.
Apoplastic histochemical features of plant root walls that may facilitate ion uptake and retention
Authors: Wu Di , Li Linbao , Li Chengdao , Dun Bicheng , Zhang Jun , Li Ten , Zhou Cunyu , Tan Debao , Yang Chaodong , Huang Guiyun , Zhang Xia 
Published: January 2022

Abstract
We used brightfield and epifluorescence microscopy, as well as permeability tests, to investigate the apoplastic histochemical features of plant roots associated with ion hyperaccumulation, invasion, and tolerance of oligotrophic conditions. In hyperaccumulator species with a hypodermis (exodermis absent), ions penetrated the root apex, including the root cap. By contrast, in non-hyperaccumulator species possessing an exodermis, ions did not penetrate the root cap. In vivo, the lignified hypodermis blocked the entry of ions into the cortex, while root exodermis absorbed ions and restricted them to the cortex. The roots of the hyperaccumulators Pteris vittata and Cardamine hupingshanensis, as well as the aquatic invasives Alternanthera philoxeroides, Eichhornia crassipes, and Pistia stratiotes, contained lignin and pectins. These compounds may trap and store ions before hypodermis maturation, facilitating ion hyperaccumulation and retention in the apoplastic spaces of the roots. These apoplastic histochemical features were consistent with certain species-specific characters, including ion hyperaccumulation, invasive behaviors in aquatic environments, or tolerance of oligotrophic conditions. We suggest that apoplastic histochemical features of the root may act as invasion mechanisms, allowing these invasive aquatic plants to outcompete indigenous plants for ions.
Model-based on-board post-injection control development for marine diesel engine
Authors: Storm Xiaoguo , Nguyen Hoang Khac , Modabberian Amin , Zenger Kai , Hyvönen Jari 
Published: January 2022

Abstract
The increasing demands for reducing fuel consumption and emissions in contemporary technology solutions lead to the use of more sensors, actuators, and control applications. With this increasing engine complexity, the feedback design is complex due to the coupling between inputs and combustion parameters. To be able to design the controller systematically, model predictive control (MPC) comes to the scope because of its advantages in the design of multi-input multi-output (MIMO) systems, especially with its constraints handling ability and performance in simultaneously optimizing the engine fuel efficiency and emission reduction. Multi-injection is one of the promising techniques for achieving better engine performance. In this work, post-injection control is implemented utilizing MPC MIMO strategy with the target of exploring the possibility of reducing emissions and improving engine efficiency by controlling post-injection duration and injection timing. The workflow of the MPC controller design from control-oriented model (COM) establishing to MPC problem formation and solution methodology is discussed in this work. Moreover, one contribution from this work is the different implementation angle when compared with the state-of-the-art approaches, where the MPC controller is implemented purely by Matlab Simulink to enable the rapid control prototyping design. The simulation result demonstrated the ability of the controller’s tracking performance and showed a preliminary step towards the nonlinear combustion model-based multi-injection MPC design. The systematic model-based controller framework developed in this work can be applied to other control applications and enables a fast path from design to test.
On exact solutions of fractional differential-difference equations with Ψ-Riemann–Liouville derivative
Authors: Ramaswamy Rajagopalan , Latif Mohamed S. Abdel , Elsonbaty Amr , Kader Abas H. Abdel 
Published: January 2022

Abstract
The aim of this work is to modify the invariant subspace method (ISM) in order to obtain closed form solutions of fractional differential-difference equations with Ψ-Riemann–Liouville (Ψ-RL) fractional derivative for first time. We have investigated the cases of two-dimensional and the three-dimensional invariant subspaces (ISs) in the suggested scheme. Using the modified ISM, new exact generalized solutions for the general fractional mKdV Lattice equation and the fractional Volterra lattice system are obtained. Compared with similar solution techniques in literature, the presented solution scheme is highly efficient and is capable to find new general exact solutions which cannot be attained by other methods.
Solutions and stability for p-Laplacian differential problems with mixed type fractional derivatives
Authors: Zhang Lingling , Zhang Nan , Zhou Bibo 
Published: January 2022

Abstract
In this note, the main emphasis is to study two kinds of high-order fractional p-Laplacian differential equations with mixed derivatives, which include Caputo type and Riemann–Liouville type fractional derivative. Based on fixed point theorems on the cone, the existence-uniqueness of positive solutions for equations and two iterative schemes to uniformly approximate the unique solutions are discussed theoretically. What’s more, the sufficient conditions for stability of the equations are given. Some exact examples are further provided to verify the analytical results at the end of the article.
Effects of miRNA-199a-5p on cell proliferation and apoptosis of uterine leiomyoma by targeting MED12
Authors: Zhao Wei , Zhao Yingyan , Chen Ling , Sun Yan , Fan Sumei 
Published: January 2022

Abstract

Background/aims
Uterine leiomyoma (ULM) is a kind of gene-involved benign tumor, which is located in the front of female reproductive tract. It is one of the most common reproductive tract tumors in women, which leads to abnormal menstruation, repeated pregnancy loss, and other serious gynecological diseases. Recently, microRNAs (miRNAs) have attracted much more attention in the process of exploring the molecular mechanisms of tumorigenesis. Furthermore, the deregulated miRNAs had been reported to play important roles in ULM pathology.

Methods
In this study, we assessed the expression level of microRNA-199a-5p (miR-199a-5p) in human ULM by quantitative polymerase chain reaction. After that cell counting kit 8, colony formation, 5-ethynyl-20-deoxyuridine, flow cytometry, and Western blot analyses were performed to investigate the effects of miR-199a-5p on ULM cell proliferation and apoptosis.

Results
We confirmed that miR-199a-5p was significantly downregulated in human ULM. The results of function analyses showed that miR-199a-5p inhibited cell proliferation and induced cell apoptosis in vitro. Bioinformatics tool showed oncogene MED12 was one of the target genes of miR-199a-5p, which mediated the effect of miR-199a-5p on the ULM.

Conclusion
Our results showed that miR-199a-5p functioned as an antitumor factor in human ULM cells. These findings broaden the current findings on the function of miR-199a-5p into the ULM pathogenesis, and miR-199a-5p may serve as a prognosis and therapeutic target for the ULM and its related diseases.
A novel function of CREG in metabolic disorders
Authors: Li Yang , Tian Xiaoxiang , Zhang Quanyu , Yan Chenghui , Han Yaling 
Published: January 2022

Abstract
Metabolic disorders are public health problems that require prevention and new efficient drugs for treatment. Cellular repressor of E1A-stimulated genes (CREG) is ubiquitously expressed in mature tissues and cells in mammals and plays a critical role in keeping cells or tissues in a mature, homeostatic state. Recently, CREG turns to be an important mediator in the development of metabolic disorders. Here in this review, we briefly discuss the structure and molecular regulation of CREG along with the therapeutic strategy to combat the metabolic disorders.