bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between muscle dysmorphia psychopathology and binge eating in a large at-risk cohort of men and women
Authors: Halioua Robin , Wyssen Andrea , Iff Samuel , Karrer Yannis , Seifritz Erich , Quednow Boris B. , Claussen Malte Christian 
Published: July 2022

Abstract
Background
Current research on muscle dysmorphia (MD) has focused on restrained eating behaviors and has adopted a primarily male perspective. Despite initial evidence, the role of possible binge eating associated with MD has only been scarcely investigated. To extend the transdiagnostic and cross-gender approaches and address the dearth in research related to MD, this study investigated the association between MD psychopathology and binge eating in men and women.

Methods
This study investigated the association between MD psychopathology and binge eating in both men and women. Participants were a sample of 5905 men (n = 422) and women (n = 5483) social media users aged 18–72 years. They completed an online survey that included self-report measures assessing demographics, binge eating, MD psychopathology, and drive for thinness and leanness. Binge eating was assessed using the diagnostic questions of the validated German version of the Eating Disorder Examination-Questionnaire. The Muscle Dysmorphic Disorder Inventory (MDDI) was used to assess MD psychopathology. A total score of > 39 was set as a cutoff to define an “MD at-risk” state for both men and women. Hierarchical logistic regression analysis was used to analyze the association between MD psychopathology and binge eating.

Results
MD psychopathology was significantly positively associated with binge eating in both men and women. Among the three MDDI subscales, only appearance intolerance was significantly associated with MD, and drive for size and functional impairment were not associated. MD at-risk status yielded a predicted probability of binge eating of 25% for men and 66.9% for women. The increased probability of binge eating associated with MD at-risk status was mainly accounted for by appearance intolerance in men and drive for thinness in women.

Conclusion
MD psychopathology is positively associated with binge eating in both men and women. Binge eating episodes should therefore form part of the clinical assessment of MD.
Dynamic change of soluble interleukin-2 receptor distinguished molecular heterogeneity and microenvironment alterations in diffuse large B-cell lymphoma
Authors: Huo Yu-Jia , Xu Peng-Peng , Wang Li , Zhong Hui-Juan , Fu Di , Shi Qing , Cheng Shu , Wang Shuo , Zhang Mu-Chen , Zhao Wei-Li 
Published: July 2022

AbstractDiffuse large B-cell lymphoma (DLBCL) is an aggressive lymphoma with variable clinical outcomes and prediction of prognosis remains important for long-term remission. We performed serial serum soluble interleukin-2 receptor (sIL-2R) measurement pretreatment and before each cycle of the treatment in 599 patients with de novo DLBCL. Genomic and transcriptomic features were analyzed by 223 DNA- and 227 RNA-sequencing, respectively. Applying the cut-off value to sIL-2R pretreatment and cycle 2 (C2) level, patients were classified into FINE subtype (pretreatment low level) with good prognosis, RES subtype (pretreatment high level and C2 low level) with intermediate prognosis, and RET subtype (pretreatment high level and C2 high level) with poor prognosis, independent of International Prognostic Index. In “others” genetic subtype, dynamic change of sIL-2R showed prognostic significance and genetic features. Compared with FINE subtype, RES subtype had increased ARID1A and MYD88 mutations, and RET subtype had increased KMT2D, LYN and SOCS1 mutations. RES and RET subtypes showed significant enrichment in oncogenic pathways, such as ERK, NF-κB, JAK-STAT, and immune-associated pathways. As for tumor microenvironment, RES subtype exhibited increased recruiting activity of CD8 + T, T helper 1, and natural killer cells, and RET subtype with increased recruiting activity of CD4 + T and regulatory T cells in silico. There was a positive correlation between transcripts of IL-2R and immune checkpoint expressions including PD-1 and CTLA-4. Our findings identified that dynamic change of sIL-2R, with this simple and easy detection method in peripheral blood, had long-term prognostic effect and specific relation to microenvironment alterations in DLBCL.
Identification and a culture method for a Helicotylenchus microlobus from tomato in China
Authors: Xia Yan-Hui , Li Jin , Xu Fei-Fei , Lei Bin , Li Hong-Lian , Wang Ke , Li Yu 
Published: July 2022

Abstract
Background
The nematodes of the genus Helicotylenchus are root parasites of a wide variety of plants, and certain species can cause serious damage to their hosts. During a survey of the plant-parasitic nematode associated with tomato, a population of Helicotylenchus was collected from tomato roots and soil samples. Thus, one of the objectives of the study was to confirm the specie of Helicotylenchus obtained from the tomato samples based on morphological and molecular characteristics. In addition, a mass pure culture of plant-parasitic nematodes is key to pathogenicity studies and many other biological studies. However, a successful mass rearing method for Helicotylenchus has not been reported. Thus, the other objective of the study was to establish a method of culturing Helicotylenchus.

Results
Based on both the morphological characteristics and molecular analysis of the internal transcribed spacer (ITS) and D2-D3 expansion region of 28S ribosomal RNA (rRNA) sequences the specimens were identified as Helicotylenchus microlobus. Phylogenetic analysis with the rRNA sequences of the ITS and 28S D2-D3 regions was consistent with molecular identification, suggesting this population formed a highly supported clade with other H. microlobus populations. Additionally, a method for culture of H. microlobus on carrot disks was established, and the effect of temperature on the reproduction rate (Rr) of H. microlobus was investigated. The optimum temperature for culturing H. microlobus on carrot disks was 27.5 °C and, after inoculation with 30 females of H. microlobus at 27.5 °C for 90 days, Rr reached 406.

Conclusions
To our knowledge, this is the first detailed description of H. microlobus from tomato in China. This study also demonstrated that the carrot disk method is suitable for the culture of H. microlobus. This study lays a foundation for other related research on H. microlobus, and has significance for the study of Helicotylenchus.
Impact of hemodialysis solutions containing different levels of molecular hydrogen (H2) on the patient-reported outcome of fatigue
Authors: Uemura Susumu , Kegasa Yoshitaka , Tada Keigo , Tsukahara Taichi , Kabayama Shigeru , Yamamoto Tae , Miyazaki Mariko , Takada Joji , Nakayama Masaaki 
Published: July 2022

Abstract
Background
Reportedly, dialysis solutions containing molecular hydrogen (H2) might ameliorate patient-reported fatigue in hemodialysis (HD) patients. However, it is unknown whether its impact might differ with different H2 levels.

Method
This single-arm, prospective observational study examined 105 patients on chronic HD (62 males; mean age, 66 years; mean HD duration, 117 months). All patients were originally treated with an HD solution with 47 ppb (mean) H2 for more than 12 months, followed by an HD solution with 154 ppb (mean) H2 for 8 weeks. Baseline and changes in subjective fatigue status rated on a numerical rating scale (NRS) were assessed before the start of the study (baseline) and 8th week of the study.

Results
Patients were classified into three groups according to the presence of subjective fatigue at baseline: Group A (15.2%), presence of fatigue on both HD and HD-free days; Group B (28.6%), fatigue only on HD days; and Group C (56.2%), freedom from fatigue. In Group A, NRS scores during the 8-week period were significantly decreased as compared with 0 week, at the 4th and 8th week on HD days, and at the 8th week on HD-free day, respectively. While no consistent changes were found in other groups. At the 8th week, 64 patients (61%) presented absence of or decrease in the NRS score of fatigue, while the rest of patients did not present the decrease in NRS (the non-improved: 39%). Regarding the factors related to the non-improved, prescription of antihypertensive agents was a significant independent risk factor by multivariate analysis, indicating the possible involvement of excess fall in blood pressure (BP) in those patients.

Conclusion
Amelioration of the patient-reported outcome of fatigue might be influenced by H2 levels in the HD solution, and the optimal H2 level in the dialysate needs to be elucidated in consideration of clinical type of fatigue and BP control status.
Diffusion-weighted imaging (DWI) and apparent diffusion coefficient (ADC) values of optic nerve head in Papilledema
Authors: Maaly Mohamed Abdel-Aziz , Hafez Kamal Y. M. K. , Habib Rehab Mohammed 
Published: July 2022

Abstract
Background
Papilledema is optic disc pathology characterized by swelling of the optic disc occurring secondary to elevated intracranial pressure (ICP), and it was proposed that elevated ICP is transferred along the subarachnoid space to the optic nerve (ON) leading to metabolic process interruptions and MRI changes within ON head. This study aimed to rule out if DWI and ADC map can be used as a diagnostic tool for detection of papilledema and if ADC values can be helpful in papilledema grading.

Result
ADC value was significantly lower in papilledema cases group (A) in comparison with control group (B).

Conclusion
Diffusion-weighted imaging (DWI) and apparent diffusion coefficient (ADC) map can be utilized as a diagnostic tool for diagnosis of papilledema, and ADC values can be helpful in diagnosis of papilledema grade.
Biatrial and interatrial septal calcification in the setting of rheumatic heart disease and mitral and tricuspid valves replacement
Authors: Dadkhah Adeleh , Borjian Mohammad Amin , Akbarzadeh Pasha Saeed 
Published: July 2022

Abstract
Background
Although cardiac calcifications are described in the literature, calcification of atria is less frequently reported. There have been few case studies about atrial wall calcification in the literature, most of which were in middle-age females and were attributable to chronic heart diseases including rheumatic heart disease and valve replacement. In majority of the reported cases, interatrial septum has been spared. Only one case of bilateral atrial wall calcification has been reported prior to the current report, which has been in a patient with renal failure, calciphylaxis and long-term haemodialysis and calcium supplement intake.

Case presentation
The patient was a 57-year-old female with history of rheumatic valvular heart disease and two prior valve replacement surgeries. She didn’t have any history of renal function impairment and haemodialysis or calcium supplements intake. Her laboratory data showed anaemia, increased cardiac biomarkers and active urinalysis. Performed echocardiography showed moderate right ventricular dilation with relatively poor contraction, normal left ventricular size and contraction, dilated right and left atria and good prosthetic valve function. On computed tomography (CT) scan, calcification of left atrial posterior and free walls, interatrial septum and right atrial free wall were noted. Evidence of hepatic congestion and cirrhosis was seen in abdominal CT scan and ultrasonography.

Conclusion
Atrial wall calcification is a rare finding but is important to report since it can complicate cardiac surgeries. It's also probable that this kind of dystrophic calcification could not be detected during routine echocardiography and CT scan should be performed in suspected cases.
Practitioners’ experience of the working alliance in a blended cognitive–behavioural therapy intervention for depression: qualitative study of barriers and facilitators
Authors: Doukani Asmae , Free Caroline , Araya Ricardo , Michelson Daniel , Cerga-Pashoja Arlinda , Kakuma Ritsuko 
Published: July 2022

Background
Digital technologies have been widely acknowledged as a potentially useful resource for increasing mental healthcare access. The working alliance is a key influence on outcomes in conventional psychotherapy, but little is known about therapists’ experiences of forming an effective working alliance in blended interventions that involve in-person psychotherapy and a digital programme.

Aims
To investigate psychological well-being practitioners’ (PWPs’) experiences of the working alliance in a trial of blended cognitive–behavioural therapy (b-CBT) for depression. Trial registration ISRCTN12388725.

Method
Semi-structured qualitative interviews were conducted with 13 PWPs who delivered b-CBT in a two-arm, non-inferiority randomised controlled trial investigating the effectiveness of b-CBT compared with face-to-face CBT. Thematic analysis was used to analyse the data.

Results
Participants reported four facilitating factors when building and maintaining a working alliance in b-CBT: having more time to deliver treatment, access to a wider toolkit, capacity to tailor components of b-CBT and receiving appropriate training and support. Participants also identified four barriers to building and maintaining a working alliance: time and resource constraints, usability challenges, limited flexibility to tailor the digital programme to patients’ needs and lack of confidence in delivering b-CBT.

Conclusions
Our study is the first specifically to investigate practitioners’ perceived facilitators and barriers to forming a working alliance in b-CBT for depression. Findings suggest that PWPs’ experiences of the working alliance can be improved by: accounting for the time required to deliver b-CBT in service workflows to reduce time pressures; increasing opportunities to tailor the digital programme through offering transdiagnostic tools and adaptable features; and providing appropriate b-CBT training and technical support.
The Bayh-Dole Act at 40: Accomplishments, Challenges, and Possible Reforms
Authors: Sarpatwari Ameet , Kesselheim Aaron S. , Cook-Deegan Robert 
Published: July 2022

AbstractMore than 40 years have passed since the enactment of the Patent and Trademark Amendment (Bayh-Dole) Act, which authorized institutions to patent inventions arising from federally funded research. Although some experts have heralded the Bayh-Dole Act as ushering in a new era of technological advances, others have been less sanguine about its impact. In recent years, the high price of prescription drugs and the patenting of COVID-19 therapeutics and vaccines developed with substantial federal government support have rekindled the debate over whether companies should receive more restricted rights to products originating with government funding. This article traces the history leading to the enactment of the Bayh-Dole Act and critically assesses its strengths and weaknesses as well as unresolved questions concerning its scope. Based on this analysis, the authors propose reforms to better align the Bayh-Dole Act with public values and health outcomes, including clarifying government-use rights, making it easier to invoke march-in rights for failure to meet health and safety needs, increasing transparency in how patents are licensed, and testing different approaches to foster the development and application of inventions.
A hypomorphic mutation in Pold1 disrupts the coordination of embryo size expansion and morphogenesis during gastrulation
Authors: Chen Tingxu , Alcorn Heather , Devbhandari Sujan , Remus Dirk , Lacy Elizabeth , Huangfu Danwei , Anderson Kathryn V. 
Published: July 2022

ABSTRACT
Formation of a properly sized and patterned embryo during gastrulation requires a well-coordinated interplay between cell proliferation, lineage specification and tissue morphogenesis. Following transient physical or pharmacological manipulations of embryo size, pre-gastrulation mouse embryos show remarkable plasticity to recover and resume normal development. However, it remains unclear how mechanisms driving lineage specification and morphogenesis respond to defects in cell proliferation during and after gastrulation. Null mutations in DNA replication or cell-cycle-related genes frequently lead to cell-cycle arrest and reduced cell proliferation, resulting in developmental arrest before the onset of gastrulation; such early lethality precludes studies aiming to determine the impact of cell proliferation on lineage specification and morphogenesis during gastrulation. From an unbiased ENU mutagenesis screen, we discovered a mouse mutant, tiny siren (tyrn), that carries a hypomorphic mutation producing an aspartate to tyrosine (D939Y) substitution in Pold1, the catalytic subunit of DNA polymerase δ. Impaired cell proliferation in the tyrn mutant leaves anterior–posterior patterning unperturbed during gastrulation but results in reduced embryo size and severe morphogenetic defects. Our analyses show that the successful execution of morphogenetic events during gastrulation requires that lineage specification and the ordered production of differentiated cell types occur in concordance with embryonic growth.
A Year at the Forefront of Engineering Photosynthesis
Authors: Johnson Sophie L. 
Published: July 2022

ABSTRACT
Multiple proof-of-principle experiments and successful field trials have demonstrated that engineering photosynthesis is a viable strategy for improving crop yields. Advances to engineering technologies have accelerated efforts to improve photosynthesis, generating a large volume of published literature: this Review therefore aims to highlight the most promising results from the period February 2021 to January 2022. Recent research has demonstrated the importance of understanding the impact of changing climates on photosynthesis to ensure that proposed engineering strategies are resilient to climate change. Encouragingly, there have been several reports of strategies that have benefits at temperatures higher than current ambient conditions. There has also been success in engineering synthetic bypass pathways, providing support for the feasibility of a synthetic biology approach. Continued developments in all areas of engineering photosynthesis will be necessary for sustainably securing sufficient crop yields for the future.
This article has an associated First Person interview with the first author of the paper.
Chromatin dynamics through mouse preimplantation development revealed by single molecule localisation microscopy
Authors: Portela Marta , Jimenez-Carretero Daniel , Labrador Veronica , Andreu Maria Jose , Arza Elvira , Caiolfa Valeria R. , Manzanares Miguel 
Published: July 2022

ABSTRACT
Most studies addressing chromatin behaviour during preimplantation development are based on biochemical assays that lack spatial and cell-specific information, crucial during early development. Here, we describe the changes in chromatin taking place at the transition from totipotency to lineage specification, by using direct stochastical optical reconstruction microscopy (dSTORM) in whole-mount embryos during the first stages of mouse development. Through the study of two post-translational modifications of Histone 3 related to active and repressed chromatin, H3K4me3 and H3K9me3 respectively, we obtained a time-course of chromatin states, showing spatial differences between cell types, related to their differentiation state. This analysis adds a new layer of information to previous biochemical studies and provides novel insight to current models of chromatin organisation during the first stages of development.
Multifunctional role of GPCR signaling in epithelial tube formation
Authors: Vishwakarma Vishakha , Le Thao Phuong , Chung SeYeon 
Published: July 2022

ABSTRACT
Epithelial tube formation requires Rho1-dependent actomyosin contractility to generate the cellular forces that drive cell shape changes and rearrangement. Rho1 signaling is activated by G-protein-coupled receptor (GPCR) signaling at the cell surface. During Drosophila embryonic salivary gland (SG) invagination, the GPCR ligand Folded gastrulation (Fog) activates Rho1 signaling to drive apical constriction. The SG receptor that transduces the Fog signal into Rho1-dependent myosin activation has not been identified. Here, we reveal that the Smog GPCR transduces Fog signal to regulate Rho kinase accumulation and myosin activation in the medioapical region of cells to control apical constriction during SG invagination. We also report on unexpected Fog-independent roles for Smog in maintaining epithelial integrity and organizing cortical actin. Our data support a model wherein Smog regulates distinct myosin pools and actin cytoskeleton in a ligand-dependent manner during epithelial tube formation.
Development of Precisely Controlled Structures Containing Main Group Elements for Preparing Superatoms
Authors: Kambe Tetsuya , Yamamoto Kimihisa 
Published: July 2022

Abstract
This review summarizes our recent studies of main group elements of boron, aluminum, gallium and bismuth units with dendrimer templates. Stepwise assembly of bismuth salts realized tunable photoluminescent materials according to the introduced amounts. Solid-state emission and switching of optical properties were demonstrated by using a shell-effect of the dendrimer. BH3 species placed in the dendrimer acted as a reductant in the nanosized space to form size-controlled metal particles. Atomicity control for AlCl3 or GaCl3 enabled a solution-phase synthesis of superatoms, which have been expected to be new building blocks for materials.
Optimization Helps Scheduling Nursing Staff at the Long-Term Care Homes of the City of Toronto
Authors: Anderson Manion , Bodur Merve , Rathwell Scott , Sarhangian Vahid 
Published: July 2022

The City of Toronto Long-Term Care Homes and Services (LTCH&S) division is one of the largest providers of long-term care in the Canadian province of Ontario, providing care to 2,640 residents at 10 homes across Toronto. Our collaboration with LTCH&S was initiated to facilitate the increasingly challenging task of scheduling nursing staff and reduce the high absenteeism rate observed among the part-time nurses. We developed a spreadsheet-based scheduling tool to automate the generation of schedules and incorporate nurses’ preferences for different shifts into the schedules. At the core of the scheduling tool is a hierarchical optimization model that generates a feasible schedule with the highest total preference score while satisfying the maximum possible demand. Feasible schedules had to abide by a set of complex seniority requirements, which prioritized more-senior nurses when allocating the available shifts. Our scheduling tool was implemented in a 391-bed home in Toronto. The tool allowed nursing managers to generate feasible schedules within a fraction of an hour, in contrast to the status quo manual approach, which could take up to tens of hours. In addition, the schedules successfully accounted for preferences with, on average, above 94% of the allocated shifts ranked as most preferred. History: This paper was refereed.