bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prognostic impact of lymph node dissection for clinically node-negative upper urinary tract urothelial carcinoma in patients who are treated with radical nephroureterectomy
Authors: Hsieh Hsiang-Chen , Wang Chun-Li , Chen Chuan-Shu , Yang Cheng-Kuang , Li Jian-Ri , Wang Shian-Shiang , Cheng Chen-Li , Lin Chia-Yen , Chiu Kun-Yuan 
Published: December 2022

Background
To evaluate the prognostic impact of lymph node dissection (LND) in patients who underwent radical nephroureterectomy (RNU) with bladder cuff excision (BCE) for clinically node-negative (cN0) upper urinary tract urothelial carcinoma (UTUC).

Methods
We retrospectively enrolled 520 patients with cN0 UTUC in a single tertiary referral center from 2000 to 2015. The patients were divided into three groups: patients with and without pathologically proved lymph node metastasis (pN1–3 and pN0, respectively) and patients without LND (pNx). We analyzed associations between overall survival (OS)/ disease-free survival (DFS)/ cancer-specific survival (CSS) and clinical characteristics.

Results
The patients were divided into three groups (pN1–3, pN0 and pNx with 20, 303, and 197 patients, respectively). OS/DFS/CSS in the pN1–3 group were significantly worse (all p<0.001) compared with the pN0 group. However, there were no significant differences between the pNx and pN0 groups. In the multivariate analyses, CSS was only affected by age [(hazard ratio (HR) = 1.03, p = 0.008]), positive surgical margin (HR = 3.38, p<0.001) and pathological T3–4 stages (HR = 4.07, p<0.001). In the subgroup analyses for patients with LND, locally advanced disease (pT3 and pT4) had significantly more metastases [T3–4: 13.91% (16/115) vs. T0–2: 1.92% (4/208), p<0.001].

Conclusions
In the pN0 group, LND for cN0 UTUC did not show therapeutic benefits in terms of DFS, CSS, and OS. However, LND with RNU allowed optimal tumor staging, through patients still had a poor prognosis. Clinically occult LN metastases were found in 6.2% of our patients.
Identification and validation of DNA methylation markers to predict axillary lymph node metastasis of breast cancer
Authors: Luo Jianguo , Chen Shaojun , Chen Jingsen , Zhou Yige , He Fei , Wang Enli 
Published: December 2022

Background
Axillary lymph node metastasis (ALNM) is one of the most important prognostic factors for breast cancer patients, and DNA methylation is involved in ALNM of breast cancer. However, the methylation profile of breast cancer ALNM remains unknown.

Methods
Breast cancer tissues were collected from patients with and without ALNM. We investigated the genome-wide DNA methylation profile in breast cancer with and without ALNM using reduced representation bisulfite sequencing (RRBS). Then, differentially methylated regions (DMRs) were verified by targeted bisulfite sequencing.

Results
A total of 21491 DMRs were identified between the lymph node positive group and negative group. Compared to the LN-negative breast cancer, LN-positive breast cancer had 10,920 hypermethylated DMRs and 10,571 hypomethylated DMRs. Then, 10 DMRs in the gene promoter region were detected by targeted bisulfite sequencing, these gene included HOXA5, PTOV1-AS1, RHOF, PAX6, GSTP1, RASGRF2, AKR1B1, BNIP3, CRMP1, ING5. Compared with negative lymph node, the promoter methylation levels of RASGRF2, AKR1B1 and CRMP1 increased in positive lymph node, while the promoter methylation level of RHOF decreased in positive lymph node. In addition, Cancer Genome Atlas (TCGA) data showed that RASGRF2, AKR1B1 and CRMP1 were low expressed in breast Cancer tissues, while RHOF was high expressed in breast Cancer tissues. Furthermore, in addition to highly methylated AKR1B1, RASGRF2 and CRMP1 gene promoters, BNIP3, GSTP1, HOXA5 and PAX6 gene promoters were also methylated in ER-positive and HER2-negative breast cancer with ALNM.

Conclusions
When compared to negative lymph node breast cancer, the positive lymph node breast cancer has a differential methylation status. Promoter methylation of RASGRF2, AKR1B1, CRMP1 and RHOF in lymph node positive breast cancer tissues was significantly different from that in lymph node negative breast cancer tissues. AKR1B1, RASGRF2, CRMP1, BNIP3, GSTP1, HOXA5 and PAX6 genes were methylated in ER-positive and HER2-negative breast cancer with ALNM. The study provides an important biological base for understanding breast cancer with ALNM and developing therapeutic targets for breast cancer with ALNM.
Validity of muscular fitness self-assessments in the ecofit smartphone application: A correlation study
Authors: Jansson Anna K. , Lubans David R. , Duncan Mitch J. , Smith Jordan J. , Bauman Adrian , Attia John , Plotnikoff Ronald C. 
Published: December 2022

Background
Mobile app-based interventions have the potential for wide-reach and therefore may be a useful tool in up-scaling physical activity interventions. In larger-scale interventions, face-to-face assessments are less cost-effective, and researchers often rely on surveys or activity trackers to assess outcomes. However, there is limited evidence of valid muscular fitness assessments that can be self-administered within mHealth interventions. As such, this study will evaluate the concurrent validity of upper and lower body muscular fitness that have been independently assessed by participants via the ecofit app, and face-to-face assessments conducted by a trained researcher.

Methods
This study compared baseline data from two muscular fitness tests from the ecofit two-armed randomised controlled trial and self-assessed data collected via the ecofit smartphone app (i.e., validated 90-degree push-up and 60-second sit-to-stand test). To assess the concurrent validity, the self-assessed push-up and sit-to-stand tests (i.e., collected via the ecofit app) were correlated using Spearman’s correlation coefficient against the researcher-assessed results (i.e., objective results collected during baseline assessment for the ecofit trial). Bland-Altman plots were also used to allow visualisation of the differences between the self- and research-assessed tests.

Results
Participants (N = 54) completed the push-up (24.1%) and sit-to-stand (100%) tests within 14-days of receiving the app. The results revealed a strong significant correlation for the push-up test (0.83, p<0.001) and a moderate significant correlation for the sit-to-stand test (0.63, p<0.001).

Conclusion
This study provides support for the concurrent validity of self-reported upper and lower body muscular fitness assessments (i.e., the push-up and sit-to-stand tests) in mHealth. While these tests may be a feasible option for large scale physical activity interventions, more research is needed to determine the generalisability of these results.
Multi-trajectory analysis of changes in physical activity and body mass index in relation to retirement: Finnish Retirement and Aging study
Authors: Lintuaho Roosa , Saltychev Mikhail , Pentti Jaana , Vahtera Jussi , Stenholm Sari 
Published: December 2022

Background
Physical activity and body mass index (BMI) have been reported to change around retirement. The objective was to examine the concurrent changes in physical activity and BMI around retirement, which have not been studied before. In addition, the associations of different demographic characteristics with these changes were examined.

Methods
The prospective cohort study consisted of 3,351 participants in the ongoing Finnish Retirement and Ageing Study (FIREA). Repeated postal survey, including questions on physical activity and body weight and height, was conducted once a year up to five times before and after the retirement transition, the mean follow-up time being 3.6 years (SD 0.7). Group-based multi-trajectory modeling was used to identify several clusters with dissimilar concurrent changes in physical activity and BMI within the studied cohort.

Results
Of the participants, 83% were women. The mean age at the last wave before retirement was 63.3 (SD 1.4) years. Four clusters with different trajectories of physical activity and BMI were identified. BMI remained stable around retirement transition in all four clusters, varying from normal weight to class II obesity. The association of BMI trajectories with physical activity levels were inverse, however, each activity trajectory showed a temporary increase during the retirement transition.

Conclusions
Retirement seems to have more effect on physical activity than BMI, showing a temporary increase in physical activity at the time of retirement.
The predictive value of abnormal P-wave axis for the detection of incident atrial fibrillation: A systematic review with meta-analysis
Authors: Chattopadhyay Rahul Kumar , Chousou Panagiota Anna , Mukherjee Trisha , Pugh Peter John , Vassiliou Vassilios S. 
Published: December 2022

IntroductionThere is growing interest in the prediction of incident atrial fibrillation (AF). The 12-lead electrocardiogram (ECG) has been a particularly rich target for possible prediction strategies.PurposeThe P-wave axis is an ECG parameter that reflects the dominant vector of atrial depolarisation and is usually 0° -75°. There is a large body of literature suggesting that AF reflects structural and conduction abnormalities of the atria, and thus the P-wave axis may represent a sensitive parameter to detect such changes.MethodsA systematic review and meta-analysis of published literature associating abnormal P-wave axis and the development of incident AF was performed. Electronic databases were systematically searched from inception to October 2021. A random-effects model with generic inverse variance weights was utilised to pool the most adjusted effect measure from each paper. A funnel plot was used to assess publication bias.ResultsAfter excluding duplicate studies, 568 studies were screened. A total of eleven studies were identified that associated an abnormal P-wave axis with the subsequent detection of AF. The eight studies that considered abnormal P-wave axis as being <0° or >75° were pooled for meta-analysis. In the pooled studies a total of 78,222 patients were included with 5656 cases of incident atrial fibrillation identified. The meta-analysis of the studies suggested that an abnormal P-wave axis was associated with a pooled risk ratio of 2.12 (95% CI 1.49 to 3.01) for the detection of incident atrial fibrillation.ConclusionThis comprehensive systematic review and meta-analysis, indicates the positive association of abnormal P wave axis and future detection of AF. Utilisation of abnormal P-wave axis, alongside other parameters, may allow clinicians to better risk-stratify individuals at increased risk of AF, and thus identify those who may benefit most from prolonged cardiac monitoring or targeted anticoagulation.
Clinical characteristics and risk factors for visual prognosis according to the types of infectious endophthalmitis
Authors: Lee Jae Jung , Jo Yeon Ji , Lee Jong Soo 
Published: December 2022

Background
Endophthalmitis is a fatal ophthalmological emergency that needs prompt diagnosis and treatment. This study aimed to evaluate the clinical characteristics and investigate risk factors for the visual prognosis of the different types of endophthalmitis.

Methods
This retrospective study included 239 eyes diagnosed with endophthalmitis at the Pusan National University Hospital between January 2006 and December 2020. All patients were classified into six groups based on the etiology of endophthalmitis: post-cataract surgery, post-vitrectomy, post-glaucoma surgery, post-intravitreal injection, endogenous, and post-trauma. Demographics and clinical characteristics such as age, sex, laterality, initial symptoms, the interval between the primary causable event and diagnosis of endophthalmitis, initial and final visual acuity, management, and culture results were reviewed and statistically analyzed. Risk factors for poor visual prognosis were also analyzed according to the type of endophthalmitis.

Results
Of the 239 cases of endophthalmitis, the most common cause was post-cataract surgery, that occurs within two weeks post-surgery. Gram-positive Staphylococcus was cultured most frequently. Fusarium was characteristically cultured from delayed post-cataract surgery endophthalmitis (14 days–6 weeks post-surgery). Post-vitrectomy endophthalmitis occurred within 3.3 days post-surgery, but post-glaucoma surgery endophthalmitis developed a long period after surgery, averaging 2,742 days. Post-intravitreal injection endophthalmitis occurred most frequently following bevacizumab injection, and Staphylococcus was most commonly isolated. For endogenous endophthalmitis, the pyogenic liver abscess was the most common underlying disease, and Klebsiella was isolated most frequently. Post-traumatic endophthalmitis mostly occurred in young men. Advanced age and poor initial visual acuity were risk factors for poor visual prognosis (P = 0.041, odds ratio = 1.024 and P < 0.001, odds ratio = 3.904, respectively, using logistic regression analysis).

Conclusion
Advanced age and initial visual acuity were risk factors for poor visual prognosis in cases of endophthalmitis caused by various etiologies. Early diagnosis and treatment of endophthalmitis are required, especially in older patients.
Perceptual visual dependence for spatial orientation in patients with schizophrenia
Authors: Razzak Rima Abdul , Jahrami Haitham , Husni Mariwan , Ali Maryam Ebrahim , Bagust Jeff 
Published: December 2022

Background
Patients with schizophrenia are reported to have vestibular dysfunction and to weigh vestibular input to a lesser extent compared to healthy controls. Such deficits may increase visual dependence (VD) for spatial orientation at a perceptual level in these patients. The aim of this study is to compare VD levels between healthy control and patients with schizophrenia and to explore associations between VD and clinical measures in these patients. Relation of VD to antipsychotic drug treatment is also discussed.

Method
18 patients with schizophrenia and 19 healthy controls participated in this study. The Rod and Disc Test (RDT) was used to create an optokinetic surround around a centrally located rod. Participants aligned the rod to their subjective visual vertical (SVV) in both static and dynamic disc conditions. VD was calculated as the difference in SVV between these two conditions.

Results
There was no group difference or gender difference in static or dynamic SVV as well as VD. There was no correlation between VD and any of the Positive and Negative Syndrome Scale (PANSS) scores, however VD was significantly correlated to illness duration in the patient group.

Conclusions
Schizophrenia is not associated with greater VD levels at a perceptual level, compared to controls, indicating adequate visuo-vestibular integration for judging line verticality in these patients. Patients with greater chronicity of the disease are more visually dependent than those less chronically ill, consistent with previous reports of possible vestibular dysfunction in patients with schizophrenia. This may affect their daily functioning in dynamic visual environments.
On the development of control solutions for local energy communities
Authors: Benigni A. , Xhonneux A. , Carta D. , Pesch T. , Muller D. 
Published: December 2022

Abstract
Local Energy Communities (LEC) have been recognized by the European Union (EU) as a key component of the energy transition. The Clean Energy Package of the European Commission has defined a legislative framework for the operation of LEC that aim at participation of LEC in energy markets. The digitalization of LECs is a critical step both for the realization of those communities as well as for their interface to system operator, service providers and markets.
In this paper, we present an incremental prototyping approach and the related infrastructure to develop control and information and communications technology (ICT) solutions for local energy communities.
The crystal structure of oxalic acid – 2-ethoxybenzamide (2/1), C20H24N2O8
Authors: Xia Ying-Fan , Lin Shu-Ting , Zhang Jia-Rong , Jiang Cheng-Jun 
Published: December 2022

Abstract
C20H24N2O8, monoclinic, P21/n (no. 14), a = 3.8981(3) Å, b = 16.0675(11) Å, c = 16.2908(10) Å, β = 94.537(3)°, V = 1017.14(12) Å3, Z = 2, R

gt
(F) = 0.0634, wR

ref
(F
2) = 0.1746, T = 170 K.
Kinderbonuskonsum
Authors: Bachmann Rüdiger , Bayer Christian , Kornejew Martin 
Published: December 2022

Zusammenfassung
Im Rahmen des deutschen Konjunkturpakets 2020 wurde eine temporäre Erhöhung des Kindergeldes, ein Kinderbonus, von 300 Euro pro kindergeldberechtigtem Kind beschlossen. Dieser Bonus wurde in zwei Raten ausgezahlt, eine im September (200 Euro) und eine im Oktober 2020 (100 Euro). Wir verwenden eine repräsentative Umfrage zum monatlichen Konsumverhalten deutscher Haushalte, um die intertemporale Konsumwirkung dieses Transfers zu schätzen. Gemäß diesen Schätzungen verausgaben die Kindergeldempfänger im Durchschnitt 28 bis 37 Prozent des Transfers als zusätzlichen Konsum bereits zwischen September und November 2020. Allerdings konzentriert sich die Ausgabensteigerung signifikant – wie auch theoretisch zu erwarten – auf Kindergeldempfänger mit niedrigem liquiden Vermögen, die erwarten, dass sie den Kinderbonus nicht vollständig über die Einkommensteuer zurückzahlen müssen. Diese Gruppe verausgabt den zusätzlichen Transfer fast vollständig.
Cluster of cases due to Shigella flexneri producing CTX-M-15 in Spain
Authors: Kocsis Erika , Díaz de Tuesta José Luis , Sánchez Juan , Santamaría Rosaura , Moragas Manuel , Herrera-León Silvia , Cisterna Ramón 
Published: December 2022

AbstractThe aim of our study was to delineate an outbreak of gastroenteritis caused by Shigella flexneri and affecting sixteen persons between May and June 2014 in Bilbao, Spain. All patients exhibited symptoms after consuming kebab in the same kebab shop.The outbreak is described through the clinical cases, the microbiological and molecular genetic diagnosis, and the epidemiologic investigation. Minimum inhibitory concentrations for ampicillin, amoxicillin plus clavulanic acid, third and fourth generation cephalosporins, carbapenems, monobactams, aminoglycosides, fluoroquinolones, co-trimoxazole, colistin and tigecycline were measured. The S. flexneri strains were screened by PCR for TEM, SHV, CTX-M beta-lactamases and plasmidic AmpCs and aac(6′)-Ib gene. Serotyping, pulsed field gel-electrophoresis, conjugation assay, plasmid sizing by S1 enzyme digestion and Southern blot hybridization were accomplished.All the S. flexneri isolates proved to be serotype 2 and produced extended-spectrum beta-lactamase (ESBL). Carbapenems, fluoroquinolones, tigecycline, colistin, and co-trimoxazole remained active antibiotics. All the strains harboured blaCTX-M-15 and blaOXA-1 genes. The strains hosted two high-molecular weight plasmids of 100 and 230 kb, respectively. According to the hybridization assay blaCTX-M-15 was located on the plasmid of 230 kb. The identical pulsotype verified the presence of outbreak.Remarkable, that one of the food handlers has travelled recently to Pakistan, where ESBL-producing Shigella strains had been reported previously. To the best of our knowledge, this is the first outbreak caused by CTX-M-15-expressing S. flexneri in Spain and as well as in Europe.
Isolation and Identification of Bacterial Endophytes from Grain Amaranth (Amaranthus caudatus) for Plant Growth Promotion
Authors: Bhagyashree K.B. , Shivaprakash M.K. , Reddy M. Raveendra 
Published: December 2022

Background: Bacterial endophytes lives in internal tissues of root, stem and leaves of grain amaranth plants. Isolation and screening of efficient bacterial endophytes for plant growth promotion, biocontrol activities and drought tolerance can develop as suitable bioinoculants for alleviate drought and stress tolerance and plant growth promotion. Methods: Bacterial endophytes were isolated from grain amaranth (Amaranthus spp) and all isolates were screened for nitrogen fixation, phosphorous solubilisation, growth hormone production and 1-aminocyclopropane-1-carboxylate (ACC) deaminase production. Molecular identification of bacterial isolates was done by 16S rRNA sequencing to identify the organisms. Result: Seventeen isolates showed high growth in N-free media and nine isolates could efficiently solubilize phosphates. Two isolates significantly produced higher concentrations of gibberellins, maximum number of bacterial isolates produced Indole-3-acetic acid (IAA) and eight isolates were able to produce cytokinins. Fifteen isolates have shown positive for ACC activity [1-Aminocyclopropane-1-carboxylate (ACC) deaminase production] in grain amaranth and five bacterial isolates (RAR-5, RAS-3, RAL-7, WAR-1 and WAS-11) have significant plant growth promoting activities compared to others. Further, significant bacterial isolates were identified as Alcaligenes faecalis subsp. phenolicus, Pseudomonas moorei, Bacillus siamensis, Bacillus cereus and Acromobacter deleyi by 16S rRNA gene sequencing.
Metabolic Profiling of Plant Growth Promoting Microorganisms (PGPMs) from Chickpea Rhizosphere and Their Antagonistic Activity against Dry Root Rot Pathogen Rhizoctonia bataticola
Authors: Patil Meghana S. , Sunkad Gururaj , Yenjeerappa S.T. , Kulkarni Sunil A. , Rao Satyanarayana , Naik Nagaraj M. 
Published: December 2022

Background: Dry root rot (DRR) caused by Rhizoctonia bataticola is the major threat to chickpea production globally. Recently, upsurge in disease incidence is observed due to unexpected increase in temperature and soil moisture stress resultant of changing climatic scenarios. To combat this necrotrophic phytopathogenic fungus, the present study was conducted with the aim of managing the disease by using potential rhizospheric PGPMs. Methods: Twenty-six isolates of soil-borne fungal PGPMs were isolated from rhizosphere of chickpea in North Eastern Karnataka zone and screened for bio-active compounds production against Rhizoctonia bataticola by inverted plate technique. Using ITS genes BLAST analysis, the putative rhizospheric fungal PGPM was identified at the molecular level. Finally, chemical characterization of the bio-active compounds and metabolic profiling was carried out using Gas Chromatography Mass Spectrometry (GC-MS) technique. Result: All the isolates showed significant differences in radial growth of mycelium at five days after incubation. SFPGPM-13 isolate produced maximum concentration of bio-active compounds with per cent mycelial inhibition of 62.83 (52.43). Molecular identification and BLAST analysis showed the isolate SFPGPM-13 was identified as Trichoderma harzianum. GC-MS analysis resulted that 62 compounds were present in the extract of SFPGPM-13 at different retention time and mass to charge (m/z) ratio.