bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility and clinical applicability of genomic profiling based on cervical smear samples in patients with endometrial cancer
Authors: Kim Namsoo , Kim Yoo-Na , Lee Kyunglim , Park Eunhyang , Lee Yong Jae , Hwang So Yoon , Park Jihyang , Choi Zisun , Kim Sang Wun , Kim Sunghoon , Choi Jong Rak , Lee Seung-Tae , Lee Jung-Yun 
Published: August 2022

PurposeCervical smear samples are easily obtainable and may effectively reflect the tumor microenvironment in gynecological cancers. Therefore, we investigated the feasibility of genomic profiling based on tumor DNA analysis from cervical smear samples from endometrial cancer patients.Materials and methodsPreoperative cervical smear samples were obtained via vaginal sampling in 50 patients, including 39 with endometrial cancer and 11 with benign uterine disease. Matched blood samples were obtained simultaneously. Genomic DNA (gDNA) from cervical smear and/or cell-free DNA from whole blood were extracted and sequenced using the Pan100 panel covering 100 endometrial cancer-related genes.ResultsCervical swab-based gDNA analysis detected cancer with 67% sensitivity and 100% specificity, showing a superior performance compared to that of the matched blood or Pap smear tests. Cervical swab-based gDNA effectively identified patients with loss of MSH2 or MSH6 and aberrant p53 expression based on immunohistochemistry. Genomic landscape analysis of cervical swab-based gDNA identified PTEN, PIK3CA, TP53, and ARID1A as the most frequently altered genes. Furthermore, 26 endometrial cancer patients could be classified according to the Proactive Molecular Risk Classifier for Endometrial Cancer.ConclusionCervical swab-based gDNA test showed an improved detection potential and allowed the classification of patients, which has both predictive and prognostic implications.
Estradiol mediates the interaction of LINC01541 and miR-429 to promote angiogenesis of G1/G2 endometrioid adenocarcinoma in-vitro: A pilot study
Authors: Qiao Dan , Qin Xiaoduo , Yang Haiyan , Liu Xuantong , Liu Libing , Liu Sufen , Jia Zhongzhi 
Published: August 2022

BackgroundEndometrioid adenocarcinoma (EAC) is the most common subtype of endometrial cancer (EC) and is an estrogen-related cancer. In this study, we sought to investigate the expressions and mechanism of action of 17β-estradiol (E2) and long noncoding RNA (lncRNA) LINC01541 in G1/G2 EAC samples.MethodsThe expressions of estrogen receptor β (ESR2), LINC01541, miR-200s, and VEGFA were evaluated using real-time PCR in human EAC tissues (n = 8) and adjacent normal tissues (n = 8). Two EC cell lines (Ishikawa and RL95-2) were selected for validation in vitro. Bioinformatics analyses and luciferase reporter analyses were performed to verify potential binding sites. qRT-PCR, Western blot, and CCK-8 were used to identify the regulatory mechanisms of related genes in cell biological behavior.ResultsCompared with adjacent normal tissues, LINC01541 and miR-200s family (except miR-200c) were highly expressed in EAC tissues (n=8), while ESR2 and VEGFA were lowly expressed in EAC tissues (* P &lt; 0.05; ** P &lt; 0.01). In vitro: E2 inhibited the expression of LINC01541 and miR-429 in both cell lines, and estrogen antagonist (PHTPP) could reverse this effect, in addition, PHTPP could promote the proliferation of these two cancer cells, cell transfection LINC01541 also had this effect after overexpression of plasmid and miR-429 mimic. E2 promotes the expression of VEGFA in both cell lines, and PHTPP can also reverse this effect. LINC01541 interacts with miR-429 to promote the expression of each other, and both inhibit the synthesis of VEGFA in EAC cells after overexpression. Through the double validation of bioinformatics analysis and dual fluorescein reporter gene, it was confirmed that miR-429 targets the regulation of VEGFA expression (* P &lt; 0.05; ** P &lt; 0.01).ConclusionE2 promotes the synthesis of VEGFA by altering the expression levels of LINC01541 and miR-429 in EAC, thereby affecting the angiogenesis process of EAC. Also, E2-mediated LINC01541/miR-429 expression may affect cell migration in EAC. In addition, we identified a reciprocal promotion between LINC01541 and miR-429.
Role of intraoperative patients positioning in endoscopic full-thickness resection of large gastric tumors under general anesthesia
Authors: Zhou Li-Jun , Xing Fei , Chen Dan , Li Yan-Na , Rafiq Shoaib Mohammad 
Published: August 2022

Full thickness endoscopic resection of large submucosal gastric tumors (&gt;3 cm) is a big challenge for endoscopists. Issues include how to efficiently resect the lesion, obtain homeostasis, and suture the defect. There are no guidelines regarding the importance of patient position on the success of endoscopic resections in anesthetized patients. Typically, the patient is placed in left lateral position for the endoscopic therapy and during the procedure patient’s position is changed to maintain the tumor above the gastric fluids to prevent gastric juices and tumor or tumor fragments from falling into the peritoneal cavity in the event of perforation. This study emphasized the importance of planning the procedure to ensure that the patient’s position and anesthetist’s concerns are met and allow optimal access to the lesion for endoscopic resection. Prior to sedation the patient should be positioned so that the tumor is in the up position which also prevents blood obscuring the operative field, helps detect bleeding points for immediately hemostasis. In addition, due to gravitational effect, the resected tumor will fall into the gastric cavity exposing the root of the tumor making resection easier and reduce procedure time. Preplanning avoids unnecessary readjustment of positioning and improves the ease and safety of the procedure.
Paclitaxel-incorporated nanoparticles improve functional recovery after spinal cord injury
Authors: Zhang Xinzhu , Xiong Wu , Kong Guang , Zhen Yushan , Zeng Qiang , Wang Siming , Chen Sheng , Gu Jun , Li Cong , Guo Kaijin 
Published: August 2022

As a worldwide medical problem, spinal cord injury has no clear and effective treatment to improve its prognosis. Hence, new treatment strategies for spinal cord injury with good therapeutic efficacy have been actively pursued. As a new drug loading system, acetal dextran nanoparticles (SAD) have good biocompatibility and biodegradability. Therefore, we designed spermine-functionalized acetal-dextran (SAD) nanoparticles and encapsulated paclitaxel (PCL) into them. This design can ensure the sustained release of paclitaxel in the injured area for 4 days and promote the extension of nerve processes in vitro. In our experiment, we found that paclitaxel-loaded SAD nanoparticles (PCL@SAD) decreased the level of chondroitin sulfate proteoglycan in the rat spinal cord injury model, which reduced the scar repair of the injured site and changed the inhibitory environment after spinal cord injury. This reveals that PCL@SAD can effectively protect the injured spinal cord and ultimately improve the functional recovery of the injured spinal cord. One single injection of PCL@SAD shows better therapeutic effect than that of PCL. This study opens an exciting perspective toward the application of neuroprotective PCL@SAD for the treatment of severe neurological diseases.
“I'm in pain and I want help”: An online survey investigating the experiences of tic-related pain and use of pain management techniques in people with tics and tic disorders
Authors: Taylor Evangeline , Anderson Seonaid , Davies E. Bethan 
Published: August 2022

ObjectivesTic disorders (TDs) are complex neurological conditions characterized by involuntary, persistent vocalizations and motor movements called tics. Tics involve brief muscle movements and can impair many aspects of daily functioning and quality of life in patients – and their physical nature can cause pain. Understanding individuals' experiences of tic-related pain and pain management could help explore this under-researched area and identify additional support needs for this population. The aim of this study was to investigate experiences of pain and use of pain management techniques in people with tic disorders.MethodsAn online survey consisting of multiple choice and open-ended questions exploring experiences of tic-related pain, help-seeking behavior for tic-related pain, and use of pain relief techniques for tic-related pain, was circulated onlineviainternational Tourette syndrome patient associations, and one online support group for Tourette syndrome. The online survey was open to adults (≥16 years) with self-reported tics. Open-ended questions were analyzed using thematic analysis.ResultsOne hundred eighty-one participants (16–71 years; 58.0% female) from 18 countries completed the online survey. Several aspects of tics were associated with pain, including the physical effort of motor tics (n= 177, 97.8%), repetitive tics (n= 141, 77.9%) and the consequences of tics (n= 131, 72.4%). Nearly two-thirds (n= 118, 64.6%) had sought professional help for tic-related pain. Distraction techniques (n= 126, 69.6%), taking pain relief medication (n= 125, 69.1%) and altering tics (n= 111, 61.3%) were the most commonly-reported methods used to relieve and cope with tic-related pain. Thematic analysis found an interrelated complex relationship between participants' tics, pain, and pain management techniques, reflected in four themes: the “tic-pain” cycle, the impact of pain, the importance of support, and the perceived successfulness of pain management techniques.ConclusionsTic-related pain was reported to have a significant physical and psychological impact which impacted aspects of daily living in people with tic disorders. The findings add to limited research suggesting tic-related pain is a dominant issue for individuals with tic disorders, potentially impacting upon their quality of life. Increased understanding of tic-related pain and its influence may be helpful in the long-term management of tic disorders, both in terms of clinical management and patients' self-management.
Effects of coronavirus disease 2019 lockdown on metabolic syndrome and its components among Chinese employees: A retrospective cohort study
Authors: Xu Weiwei , Li Yujuan , Yan Yixin , Zhang Liyun , Zhang Junhui , Yang Chao 
Published: August 2022

ObjectiveCoronavirus disease 2019 (COVID-19) and the accompanying isolation have changed resident life rhythms and behaviors. This study investigated the effects of the COVID-19 pandemic on metabolic syndrome (MetS) and its components in employees in southwestern China.MethodsThis retrospective cohort study included 3,777 employees of five institutions who underwent physical examinations at the Affiliated Hospital of Southwest Medical University for three consecutive years from 2018 to 2020. We collected data on participant age and sex and measured the component indices of metabolic syndrome, including waist circumference, blood pressure (systolic and diastolic), fasting blood glucose level, and blood lipid (triglyceride and high-density lipoprotein cholesterol) level. We applied t-, chi-square, Mann–Whitney U, and Friedman's M tests to compare metabolic variables at different times.ResultsThe incidence of MetS in 2020 was 18.6%, significantly higher than the prevalence of 15.7% before the epidemic. The number of abnormal MetS components following the COVID-19 lockdown was much greater than those in 2018 (P &lt; 0.001) and 2019 (P &lt; 0.001), with no significant variations between the two years (P = 0.142). All metabolic parameters, except for fasting blood glucose, were significantly worse than those pre-lockdown. The increase in the prevalence of MetS and all its abnormal components except for fasting glucose from 2019 to 2020 was significantly higher than that from 2018 to 2019. The change values between 2019–2020 and 2018–2019 for all indices except for diastolic blood pressure did not differ significantly between men and women. For all component indicators except for waist circumference, we observed no significant age differences in the growth differentials between the two periods (2019–2020 and 2018–2019).ConclusionsCOVD-19 lockdown have increased metabolic health risks among Chinese adults. Targeted measures, such as health education, are urgently needed to address poor metabolic health caused by the COVID-19 pandemic.
Investigating the relationship between cancer and orofacial clefts using GWAS significant loci for cancers: A case-control and case-triad study
Authors: Fashina Azeez , Busch Tamara , Young Mary , Adamson Olawale , Awotoye Waheed , Alade Azeez , Adeleke Chinyere , Hassan Mohaned , Oladayo Abimbola M. , Gowans Lord J. J. , Eshete Mekonen , Naicker Thirona , Olotu Joy , Adeyemo Wasiu L. , Butali Azeez 
Published: August 2022

BackgroundSeveral population-based case-control studies have reported concurrent presentation of cancer and congenital malformations. Many associations have been made between oral clefting and cancers, though some of these results are conflicting. Some studies have reported an increased risk of cancer among 1st-degree relatives of cleft cases and vice versa, and also an excess risk of cancers of the breast, lung, and brain among those with oral clefts. This study aimed to determine if the genetic polymorphisms found in some cancers are also associated with orofacial cleft in an African cohort.MethodsThe study was a case-control and case-triad study in which cases were 400 individuals clinically diagnosed with non-syndromic cleft lip and/or palate (CL/P), while controls were 450 individuals without CL/P. Samples were obtained from three African countries while DNA extraction, PCR, and genotyping were carried out at the University of Iowa, US. Eleven SNPs in genes coding for SWI/SNF subunits and 13 GWAS significant SNPs for cancers associated with orofacial cleft were selected. Case-control analysis, transmission disequilibrium test (TDT), and DFAM to combine the parent-offspring trio data and unrelated case/control data in a single analysis were carried out using PLINK.ResultsFor the case-control analyses that included all the clefts and for the CLP subtype, none of the SNPs were statistically significant. Statistically increased risk for the following SNPs rs34775372 (p = 0.02; OR = 1.54, CI:1.07–2.22), rs55658222 (p = 0.009; OR = 2.64, CI:1.28–5.45) and rs72728755 (p = 0.02; OR=2.27, CI:1.17–4.45) was observed with the CL only sub-group. None of these were significant after Bonferoni correction. In the TDT analyses, a significantly reduced risk with rs10941679 (p = 0.003; OR = 0.43, CI:0.24–0.75) was observed and this was significant after Bonferroni correction. The rs10941679 was also significant (p = 0.003) in the DFAM analyses as well even after Bonferroni correction.ConclusionThe results from this study represent an important starting point for understanding the concurrent presentation of some cancers in orofacial clefts, and cancer risks in cleft patients. The associations observed warrant further investigation in a larger cohort and will set the stage for a more mechanistic approach toward understanding the risk for cancers in families with clefts.
Efficacy and satisfaction of preoperative telemedicine evaluation of pediatric urologic patients
Authors: Canon Stephen , Whitt Jedidiah , Spray Beverly , Patel Ashay , Zamilpa Ismael 
Published: August 2022

IntroductionMinimal data exist on the accuracy of preoperative assessment with telemedicine compared to in-person preoperative evaluation. The study compared the efficacy of telemedicine in a clinic or home setting with an in-person approach for pediatric patients with urologic surgical problems.MethodsPreoperative pediatric urologic telemedicine patients between 5 days and 7 years evaluated at our institution were reviewed between January 2011 and June 2020. Both traditional clinic telemedicine patients (TM) and home telemedicine patients (TMH) were included and compared with an in-person (IP) control cohort. Caregivers were invited to complete a National Research Council (NRC) satisfaction phone survey. Data on demographics, preoperative diagnosis/surgical plan, diagnosis at surgery, and surgical procedure were collected.ResultsNinety-six patients with completed surveys were included in the study (34 TM, 24 TMH, and 38 IP). The median age was 10 months TM, 4 months TMH, and 6 months IP. Ethnicity was predominantly non-Hispanic white: 82.4% TM, 91.7% TMH, and 89.5% IP (p = 0.413). Preoperative assessment accuracy was equivalent in the three groups, with 29/34 (85.3%) TM patients, 22/24 (91.7%) TMH patients, and 35/38 (92.1%) IP patients having an accurate surgical plan (p = 0.69). Overall caregiver satisfaction was equivalent (p = 0.231).ConclusionUse of telemedicine for pediatric urological surgical planning may be as effective in accuracy and caregiver satisfaction as in-person evaluation.
Comparative efficacy of radiofrequency denervation in chronic low back pain: A systematic review and network meta-analysis
Authors: Li Han , An Junyan , Zhang Jun , Kong Weijian , Yun Zhihe , Yu Tong , Nie Xinyu , Liu Qinyi 
Published: August 2022

BackgroundFacet joint pain is a common cause of chronic low back pain (CLBP). Radiofrequency (RF) denervation is an effective treatment option.PurposeA systematic review and network meta-analysis (NMA) was performed to evaluate and compare the efficacy and effectiveness of different RF denervation treatments in managing facet joint-derived CLBP.MethodsThe Cochrane Library, Embase, PubMed, and China Biology Medicine were searched to identify eligible randomized controlled trials (RCTs) from January 1966 through December 2021. Interventions included conventional radiofrequency denervation (CRF), pulsed radiofrequency denervation (PRF), pulsed radiofrequency treatment of the dorsal root ganglia (PRF-DRG), radiofrequency facet capsule denervation (RF-FC), and radiofrequency ablation under endoscopic guidance (ERFA). The outcome was the mean change in visual analog scale (VAS) score from baseline. A random-effects NMA was used to compare the pain relief effects of the interventions over the short term (≤6 months) and long term (12 months). The rank of effect estimation for each intervention was computed using the surface under the cumulative ranking curve.ResultsA total of 10 RCTs with 715 patients met the inclusion criteria. Moderate evidence indicated that CRF denervation had a greater effect on pain relief than sham control in the short term (standardized mean difference (SMD) −1.58, 95% confidence intervals (CI) −2.98 to −0.18) and the long term (SMD −4.90, 95% CI, −5.86 to −3.94). Fair evidence indicated that PRF denervation was more effective than sham control for pain over the long term (SMD −1.30, 95% CI, −2.17 to −0.43). Fair evidence showed that ERFA denervation was more effective for pain relief than sham control in the short term (SMD −3.07, 95% CI, −5.81 to −0.32) and the long term (SMD −4.00, 95% CI, −4.95 to −3.05). Fair evidence showed that RF-FC denervation was more effective for pain relief than sham control in the long term (SMD −1.11, 95% CI, −2.07 to −0.15). A fair level of evidence indicated that PRF-DRG denervation was more effective for pain relief than sham control in the short term (SMD −5.34, 95% CI, −8.30 to −2.39).ConclusionRF is an effective option for patients diagnosed with facet joint-derived CLBP.Systematic Review Registration: Identifier: CRD42022298238.
Health Risk Behaviours by Immigrants’ Duration of Residence: A Systematic Review and Meta-Analysis
Authors: Juárez Sol P. , Honkaniemi Helena , Gustafsson Nina-Katri , Rostila Mikael , Berg Lisa 
Published: August 2022

Objectives: The aim was to systematically review and synthesise international evidence on changes in health risk behaviours by immigrants’ duration of residence.Methods: We searched literature databases for peer-reviewed quantitative studies published from 2000 to 2019, examining alcohol, drug and tobacco use; physical inactivity; and dietary habits by duration of residence.Results: Narrative synthesis indicated that immigrants tend to adopt health risk behaviours with longer residence in North America, with larger variation in effect sizes and directionality in other contexts. Random-effects meta-analyses examining the pooled effect across all receiving countries and immigrant groups showed lower odds of smoking (OR 0.54, 0.46–0.63, I2 = 68.7%) and alcohol use (OR 0.61, 0.47–0.75, I2 = 93.5%) and higher odds of physical inactivity (OR 1.71, 1.40–2.02, I2 = 99.1%) among immigrants than natives, but did not provide support for a universal trend by duration of residence.Conclusion: Findings suggest that duration of residence could serve as an effective instrument to monitor immigrants’ health changes. However, differences in receiving country contexts and immigrant populations’ composition seem to be important to predict the level and direction of behavioural change.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/, PROSPERO CRD42018108881.
The Effect of Educational Dietary Approaches to Stop Hypertension on Blood Pressure Changes in the Elderly in the Work Area of the Kutalimbaru Health Center Deli Serdang Regency in 2022
Authors: Simorangkir Lindawati , Ginting Agustaria , Veira Uli 
Published: August 2022

Abstract
Hypertension is a non-communicable disease if not controlled can lead to complications of stroke and even death. The purpose of this study was to determine the effect of dietary approaches to stop hypertension on changes in blood pressure in the elderly in the work area of ​​the Kutalimbaru Health Center, Deli Serdang Regency in 2022. The research method used correlation with a one group pretest - posttest design. of 31 respondents, with purposive sampling technique. The data was taken directly using the observation sheet. The results of the study found that the mean pretest was 155.15 mm Hg where the standard deviation was 9.616, while the posttest mean was 147.74 mm Hg with a standard deviation of 8.835. The results of the research before and after the DASH intervention was given the Wilcoxon rank test statistical test results obtained a p value of <0.05 where the effect of DASH education on changes in blood pressure in the elderly in the working area of ​​the Kutalimbaru Public Health Center, Deli Serdang Regency in 2022. It is hoped that the results of this study can used as material when conducting education about the DASH diet for people with hypertension when conducting research, especially the elderly at the Kutalimbaru Public Health Center, Deli Serdang Regency because many respondents did not know about the DASH diet pattern
URGENSI PENDIDIKAN KEAGAMAAN DAN PEMBINAAN KESEHATAN MENTAL ANAK
Authors: Rahim Ratna , Hasnahwati Hasnahwati 
Published: August 2022

Peneliti menggunakan desain penelitian deskriptif kualitatif yang menjelaskn secara eksploratif, bertujuan menguraikan sifat dan karakteristik suatu fenomena yang ada. Selanjutnya peneliti ini menganalisis data yang diperoleh dengan teknik deskriptif, interpretatif, dan teknik komperatif. Hasil penelitian ini menunjukkan bahwa: 1. Hubungan pembinaan kesehatan mental dan pendidikan keagamaan dapat terjalin dengan baik karena hubungan sesama guru terjalin dengan kerjasama dalam pengembangan mental siswa dan untuk peningkatan prestasi belajar siswa. 2. Langkah-langkah orang tua dan guru terhadap kesehatan mental anak dalam peningktan prestasi belajar siswa dengan mengoptimalkan bentuk pembinaan kesehatan mental seperti menjalin komuniksasi, mempererat kasih sayang, mananamkan tingkah laku dan teladan kepada anak.