bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drill Bit Stick-Slip Vibration Experiment Research and Analysis of Influencing Factors
Authors: Ba Dejun , Chen Guangwu , Li Peng , An Kexin , Liu Xiaobao 
Published: January 2022

Abstract
Stick-slip vibration of the bit in ultra-deep wells will seriously affect drilling potency, increase extra costs, and even cause drilling accidents. The study of the inner mechanism and external influencing factors of stick-slip vibration is of nice significance to the analysis on the suppression of stick-slip vibration. During this paper, Firstly, the interaction between the bit and rock stratum is analyzed through theoretical analysis. Based on the principle of J. Boussinesq elastic theory solution and Coulomb-Navier criterion, the damage process of the rock and the critical conditions for the incidence of stick-slip vibration of the bit were analyzed. Next, an analysis of the axial force variation of the drill column was carried out based on the establishment of an equivalent axial force variation model of the drill column torque transmission system, and the placement most strongly affected by the stick-slip vibration was located. Then, from the perspective of energy transformation of stick-slip vibration, it is analyzed that the undamped state of the bit at the instant of rock breaking is an important factor leading to the incidence of stick-slip vibration. Finally, it is verified through semi-physical experiments that adding damping to the bit at the instant of rock breaking can directly and effectively suppress the incidence of stick-slip vibration.
Four and a half LIM domain protein 1 as a Novel Prognostic Biomarker and Correlation with Immune Infiltration Levels in Lung Adenocarcinoma
Authors: Zhang Jingtao , Guo Minghao , Zhang Jing , Zhang Guangming , Sun Ning , Cui Wenqiang , Xu Fei 
Published: January 2022

Abstract
Background: We aimed to investigate the prognostic value of Four and a half LIM domain protein 1 (FHL1) and its correlation with FHL1 and tumor-infiltration immune cells (TIICs) in lung adenocarcinoma (LUAD). Methods: FHL1 expression status and its influence on clinical characteristics in LUAD and Lung squamous cell carcinoma (LUSC) were collected based on GEPIA, TCGA, GEO, CPTAC, the HPA database, and the GTEx Portal. The ROC curve and Kaplan-Meier plots were used to assess the value of FHL1 expression levels in the diagnosis and prognosis of LUAD and LUSC. The interaction network revealed the related genes and proteins of FHL1 by GeneMANIA and STRING. The functional enrichment analysis based on FHL1 and FHL1-related differentially expression genes (DEGs) was conducted by the “clusterProfile” package and Metascape, respectively. The correlation analysis between FHL1 expression and tumor immunity was performed using TISIH, TIMER, and TISIDB. cBioPortal was used to investigate the mutation status between FHL1 and representative immune checkpoints. Results: The results showed that FHL1 expression was significantly lower in tumors relative to adjacent standard samples, and downregulated FHL1 predicted a worse prognosis for LUAD than that for LUSC. Additionally, FHL1 participated in the interleukin 15 mediated signaling pathway, response to interleukin-9, and neutrophil-mediated cytotoxicity. It was also positively correlated with TIICs (B cells, CD8+T, CD4+T cells, macrophages, neutrophils, and DC), immune checkpoints (CD80, CD48, VTCN, and PVR), and chemokines (CCL5, CCL17, CCL20 and CXCL8). Conclusion: FHL1 is a powerful prognostic biomarker of immune infiltration.
A novel theoretical approach for characterizing pore size distribution of wood cell wall using DSC technique
Authors: ZHONG XIANG , Ma Erni 
Published: January 2022

Abstract
The pore size distribution of wood cell wall is crucial for wood modification and drying in practice. This study proposed a novel approach to characterize continuous cell wall pore size distribution by solving the differential equation of the water amount melting inside wood obtained from the differential scanning calorimetry (DSC) technique. Compared with the method using the isothermal process, this approach gained a more accurate pore size distribution, and overcome the disadvantages of long-time measurement. Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) was investigated with this novel method to analyze the cell wall pore size distribution at the fiber saturation point (FSP). The results showed that most pores of the cell wall were below 10 nm. With the increase of pore size, the pore volume decreases sharply, and the cell wall pores above 20 nm hardly existed. The relation between cell wall pore size and volume had the similar trend between the discontinuous and continuous methods. However, there was a quantity gap in pore volume of 9.4-52.4 times between the two methods.
Ab Initio Determination on Diffusion Coefficient and Viscosity of FeNi Fluid Under Earth’s Core Condition
Authors: Li Wei-Jie , Li Zi , Zhang Ping , Lu Yong , Wang Cong , Ma Zhe , Jia Qian , Cheng Xue-Bin , Hu Han-Dong 
Published: January 2022

Abstract
The Earth’s outer core is mainly composed of Fe and Ni. The compositional stratification and geodynamo of the Earth’s core are closely correlated with the transport properties of the fluid in the Earth core. We selected the typical FeNi fluid, and systemically calculated its diffusion coefficient and viscosity under Earth’s core condition by quantum molecular dynamics simulation. The diffusion coefficients and viscosity are almost constant along the core adiabatic curve. The self-diffusion coefficients of Ni along the core adiabatic range from 8.37\(\times\)10−9 m2s−1 to 8.87\(\times\)10−9 m2s−1. The diffusion coefficient increases with temperature increase, while viscosity decrease with temperature increase. The calculations on the transport properties suggest that the Ni impurities have a negligible effect on the diffusion coefficient and viscosity of Earth’s core. Combining the self-diffusion coefficient of Ni and the diffusion equation, the thickness of compositional stratification at the top of the core is 138.5 km.
Neuromodulatory Role of L-arginine; Nitric Oxide Precursor Against Thioacetamide Induced-Hepatic Encephalopathy in Rats via Downregulation of Nrf-2/NF- κB -mediated Apoptosis
Authors: Sedik Ahmed , Hassan Azza , Saleh Dalia 
Published: January 2022

Abstract
The goal of the current study was to investigate the impact of arginine (ARG); a NO precursor, on thioacetamide (TAA)-induced HE. To achieve this goal, rats received TAA (100 mg/kg, i.p) thrice weekly for six consecutive weeks to induce HE. TAA-injected rats were administered ARG (100 mg/kg/day; p.o.) concurrently with TAA for six consecutive weeks. Blood was then withdrawn and rats were sacrificed, liver and brain tissues were isolated. Results of the present study demonstrated that ARG administration to TAA-induced HE in rats revealed a restoration in the serum and brain ammonia levels as well as serum AST, ALT, ALP and T. bilirubin levels as well as behavioral alterations evidenced by restoration in locomotor activity, motor skill performance, and memory impairment. ARG showed also attenuation in hepatic and brain biochemical biomarkers, pro-inflammatory cytokines and oxidative stress biomarkers. All these results were confirmed by histopathological evaluation as well as ultra-structural image of the cerebellum using a transmission electron microscope. Furthermore, treatment with ARG exhibited ameliorated immunological reactivity of Nrf2 and cleaved caspase-3 proteins in cerebellum and hepatic tissue. From all the previous findings, it can be concluded that ARG showed a beneficial role in modulating the adverse complications associated with TAA-induced hepatic encephalopathy in rats via reducing hyperammonemia and downregulating ROS and Nrf-2/NF- κB -mediated apoptosis.
Risk factors and prognostic nomogram for patients with second primary cancers after lung cancer using classical statistics and machine learning
Authors: luo lianxiang , Lin Haowen , Lin Baixin , Huang Fangfang , Luo Hui 
Published: January 2022

Abstract
Background: Previous studies have revealed an increased risk of secondary primary cancers (SPC) after lung cancer. The prognostic prediction models for SPC patients after lung cancer are particularly needed to guide screening. Therefore, we study retrospectively analyzed the Surveillance, Epidemiology, and End Results (SEER) database using classical statistics and machine learning to explore the risk factors and construct a novel OS prediction nomogram for patients with SPC after lung cancer.Methods: Data of patients with SPC after lung cancer, covering 2000 to 2016, were gathered from the Surveillance, Epidemiology, and End Results (SEER) database. The incidence of SPC after lung cancer was calculated by Standardized incidence ratios (SIRs). Cox proportional hazards regression, Kaplan-Meier methods and log-rank tests were conducted to identify the independent prognostic factors for predicting OS. These significant prognostic factors were used for the development of an OS prediction nomogram. Results: Totally, 10487 SPC samples gathered from the SEER database were divided into training cohort and validation cohort (score construction and internal validation) at random. Multivariate Cox regression analysis showed that age, sex, race, grade, marital status, CT (chemotherapy), TNM stage, radiation, lung cancer surgery and SPC surgery were independent risk factors for OS in SPC patients. The prognostic nomogram we constructed was also for OS. The concordance index (C-index) in the training cohort and validation cohort were 0.715(95%CI:0.710-0.718) and 0.716(95%CI:0.712-0.720). Additionally, calibration curves and decision curve analysis (DCA) curves revealed that the nomogram has excellent clinical utility. In the random forest (RF) model, SPC site and T stage were the two most important variables, which was consistent with the nomogram. And, the random forest (RF) model had slightly better prediction performance than the Cox risk regression model (AUC: 0.748 vs. AUC: 0.719).Conclusions: The prognosis characteristics of SPC following the lung cancer was systematically reviewed. The nomogram we constructed can provide survival predictions to assist clinicians in making individualized decisions for appropriate treatment.
E3 Ubiquitin Ligase TRIM9 Ubiquitin Modified-Beclin1 Regulates Autophagy to Relieve Myocardial Ischemia Reperfusion Injury
Authors: Wang Limeiting , Wang Nan , Rao Peng , Li Changyan , Liu Xiang , Song Wenting , Sun Lin , Lu Di 
Published: January 2022

Abstract
TRI partite motif (TRIM) family proteins are RING finger domain-containing proteins, which are related with the maintenance of cellular functions. TRIM9, a pivotal E3 ubiquitin ligase, has been implicated in the pathogenesis of many diseases. However, the role of TRIM9 in myocardial ischemia-reperfusion injury (MIRI) remains unknown. In this study, we found that TRIM9 expression is up-regulated in MIRI in vivo and in vitro model. The inhibition of TRIM9 expression further increased the protein levels of LC3II and p62, while they were elevated in the process of MIRI, suggesting the disruption of autophagic flow. Mechanistically, TRIM9 down-regulated the expression of Beclin1, a crucial protein in autophagy, and promoted the ubiquitination of Beclin1, followed by the decreased expression of LC3II and p62. Collectively, our data revealed that TRIM9 can protect against MIRI and might be a novel and potential therapeutic target for cardiovascular disease.
A strategy to achieve the inherently flame-retardant PA56 by copolymerization with DDP
Authors: Yang Tingting , Gao Yuanbo , Liu Xiucai , Wang Xueli , Yu Jianyong , Huang Shuohan , Ma Bomou , He Yong 
Published: January 2022

Abstract
As a bio-based polyamide, PA56 is restricted in many fileds due to its flammability. In this research, flame-retardant PA56 copolymers (FRPA56s) were designed and successfully synthesized with the introduction of 9,10-dihydro-10-[2,3-di(hydroxy carbonyl)propyl]-10-phosphaphenanthrene-10-oxide (DDP) unit. The thermal properties, thermal degradation and flame behavior of the resultant copolymer were investigated by DSC, TGA, LOI, UL-94 and cone calorimeter tests, respectively. Compared to PA56, the peak value of heat release rate and total heat release of FRPA56 containing 1.2 mol% DDP decrease by 35 % and 19 %, respectively, indicating the high flame-retardant efficiency of DDP. It is also found that FRPA56 with only 1.0 mol% DDP passes V-0 grade in the UL-94 test while its thermal performance is almost the same as homopolymer PA56. The pyrolysis products in both the condensed and gas phases were further systematically analyzed by SEM, Raman, X-ray photoelectron spectroscopy and TGA-GC/MS. The flame-retardant mechanism was also proposed for FRPA56s.
Integrated Transcriptome Analysis for the Hepatic and Jejunal Mucosa Tissues of Broiler Chickens Raised Under Heat Stress Conditions
Authors: Kim Deok Yun , Lim Byeonghwi , Kim Jun Mo , Kil Dong Yong 
Published: January 2022

Abstract
Background

Heat stress (HS) is one of the most important threats for the current poultry industry. Therefore, many efforts have been made to ameliorate the adverse effect of HS on poultry production; however, physiological and molecular mechanisms pertaining to HS are still limited in poultry. Therefore, the objective of the current study was to investigate functional alterations based on individual and integrated transcriptomes in the liver and jejunal mucosa tissues of broiler chickens exposed to HS conditions.
Results

Broiler chickens exposed to HS showed decreased growth performance and increased corticosterone concentrations in the feather. In the transcriptome analysis, the number of differentially expressed genes (DEGs) were identified in the liver and jejunal mucosa by HS conditions. In the liver, genes related to amino acid oxidation, tryptophan metabolism, lipid metabolism, oxidative phosphorylation, and immune responses were altered by HS, which support the reason why heat-stressed poultry had decreased productive performance. In the jejunal mucosa, genes related to defense systems, glutathione metabolism, detoxification of xenobiotics, and immune responses were differently expressed by HS conditions. The integrated transcriptome analysis with DEGs found in the liver and jejunal mucosa showed a considerable connectivity between the core nodes in the constructed networks, which includes glutathione metabolism, xenobiotic metabolism, carbon metabolism, and several amino acid metabolisms.
Conclusions

The core network analysis may indicate that increased requirement of energy and amino acids in the jejunal mucosa of broiler chickens exposed to HS conditions is likely compromised by increased oxidation and synthesis of amino acids in the liver. Therefore, our results may provide comprehensive insights for molecular and metabolic alterations of broiler chickens raised under HS conditions, which can aid in the development of the novel strategies to ameliorate the negative effect of HS on poultry productivity and health.
Transmission of Human Papillomavirus Type 124 from Horses to Humans Working on a Stud Farm
Authors: Tong Panpan , Palidan Nuerlan , Song Xiaozhen , Jia Chenyang , Deng Haifeng , Duan Ruli , Tian Shuyao , Yang Enhui , Pan Juanjuan , kuang Ling , Jia Erken , Xie Jinxin 
Published: January 2022

Abstract
Human papillomavirus type 124 (HPV124) is a betapapillomavirus that is associated with viral warts, actinic keratosis, and squamous cell carcinoma incidence. No reports of HPV124 infection in mainland China have been reported to date. In a survey of Chinese horses, we detected HPV124 via next-generation sequencing (NGS) in nasal swabs from 4% (2/56) of thoroughbred horses, and 7% (2/30) of the Akhal-Teke horses, suggesting that horses can serve as a reservoir species for this virus. In addition, HPV124 was detected in 17/50 lung tissue samples from aborted Yili horse fetuses that were initially found to be negative for equid herpesviruses and equine arteritis virus, suggesting that this pathogen may potentially contribute to abortion incidence among horses. Further investigation showed that all workers involved in Yili mare production of farm B were positive for HPV124, while all other assessed individuals in farm B or without any history of contact with horses were negative for this virus. indicating that this HPV124 strain may be transmissible from horses to human workers at an HPV124-positive farm. Sequence analyses of E6 gene sequences from 18 Chinese HPV124 samples were performed, revealing that the 7 of 18 HPV124 strain identified among horses in the present study shared 100% sequence identity with previously published HPV124 isolate. Phylogenetic analyses based on E6 gene sequences in GenBank further revealed that the HPV124 isolate identified herein was closely related to the NJ3900 HPV124 reference strain, clustering together with the established Beta 1 HPV lineage. These results provide new evidence that HPV124 can infect Chinese horses, may function as a causative agent for abortions in Yili horses, and may also infect humans laboring in Yili mare stables.
What is the Link Between Emotional Intelligence and Emotion Regulation? Behavioural and Resting-State Functional Connectivity Evidences
Authors: Zanella Federico , Monachesi Bianca , Grecucci Alessandro 
Published: January 2022

Abstract
A converging body of behavioural findings supports the hypothesis that the dispositional use of Emotion Regulation (ER) strategies depends on trait Emotional Intelligence (trait EI) levels. Unfortunately, neuroscientific investigations of such relationship are missing. To fill this gap, we analysed behavioural and resting state data from 79 healthy participants to corroborate whether the same neural circuit predicting trait EI, also predicts specific ER strategies. An unsupervised machine learning approach (Independent Component Analysis) was used to decompose resting-sate functional networks and to assess whether they predict trait EI and specific ER strategies. Behavioural results showed that total trait EI index significantly predicts and negatively correlates with the frequency of use of typical dysfunctional ER strategies (suppression and self-blame). Crucially, we observed that an increased BOLD temporal variability within sensorimotor and language networks predicts both high trait EI and the frequency of use of cognitive reappraisal. By contrast, a decreased variability in language network predicts the use of suppression. These findings support the tight connection between high trait EI and individual tendency to use functional ER strategies, and provide the first evidence that modulations of BOLD temporal variability in specific brain networks may be pivotal in explaining this relationship.
Oxidative Stress Induced Endothelial Cells-Derived Exosomes Accelerate Skin Flap Survival Through Lnc NEAT1-Mediated Promotion of Endothelial Progenitor Cell Function
Authors: Guo Linlin , Chen Yuxuan , Feng Xiaoling , Sun Di , Sun Jiaming , Mou Shan , Zhao Kangcheng , An Ran 
Published: January 2022

Abstract
Background: Flap transplantation iscommonly used in reconstructive surgery. A prerequisite for skin flap survival is sufficient blood supply.However, such approaches remain unclear. This study aimed to explore theunderlying mechanisms of exosomes derived from human umbilical vascular endothelial cells (HUVECs) exposed to oxidative stress on endothelial progenitor cells (EPCs) and their subsequent influence on the survival of skin flaps.Methods: HUVECs were treated with various concentrations of H2O2 to establish an oxidative stress model. To investigate the effects of H2O2-HUVEC-Exos and HUVEC-Exos, Cell Counting Kit-8 (CCK8), tube formation, invasion assays, and Quantitative real-time polymerase chain reaction (qRT-PCR) were performed in EPCs. Arat skin flap model was used to determine the role of the exosomes in skin flap survival in vivo. Microarray analysis was used to reveal the differentially expressed long non-coding RNAs (lncRNAs)inthe H2O2-HUVEC-Exos and HUVEC-Exos. Furthermore, gene silencing and western blotting were employedto determine the mechanism behindlncRNAnuclear enrichment enriched transcript 1 (LncNEAT1)in EPCs.Results: HUVECs were stimulated with 100 μmol/L H2O2 for 12 h to establish an oxidative stress model. H2O2-HUVEC-Exos promoted the proliferation, tube formation, and invasion of EPCsand remarkably increased skin flap survival compared to the HUVEC-Exosand control groups. Sequencing of exosome RNAs revealed that the LncNEAT1 level was dramatically increased in the H2O2-HUVEC-Exos, leading to activation of the Wnt/β-catenin signaling pathway. Comparatively,knockdown of Lnc NEAT1 in HUVEC-Exos suppressed the biological behavior of the EPCs and inhibitedWnt/β-catenin signalingpathway, which could be partially reversed with use of Wnt agonist.Conclusions: Exosomes derived from HUVECs stimulated by oxidative stress significantly promoted the pro-angiogenic ability of EPCs through the Wnt/β-cateninsignaling pathway mediated by Lnc NEAT1 and hence enhanced random flap survivalin vivo. Therefore, the application of H2O2-HUVEC-Exosmay serve as an alternative therapy for improving random skin flap survival.
Pain pupillary index to prognosticate outcome in comatose cardiac arrest patients
Authors: Macchini Elisabetta , Bertelli Alessandra , Bogossian Elisa Gouvea , Annoni Filippo , Minini Andrea , Cornejo Armin Quispe , Creteur Jacques , Taccone Fabio Silvio , Peluso Lorenzo 
Published: January 2022

Abstract
Background: The Neurologic Pupil Index (NPi), derived from automated pupillometry, can accurately predict poor neurological outcome in post-anoxic brain injury. The prognostic role of another index, called Pupillary Pain Index (PPI), remains unknown in this setting. Methods: Monocentric study in adult comatose cardiac arrest (CA) patients. Quantitative PPI and NPi were concomitantly recorded on day 1 and day 2 after CA. The primary study outcome was to assess the prognostic value of PPI to predict 3-month unfavourable outcome (UO, defined as Cerebral Performance Category of 3–5). Secondary outcome was the agreement between PPI and NPi to predict unfavourable outcome. Results: A total of 102 patients were included; 69 patients (68%) patients had UO. Patients with UO showed a lower NPi (4.2 [3.5-4.5] vs. 4.6 [4.3-4.7]; p<0.01 on day 1 - 4.3 [3.8-4.7] vs 4.6 [4.3-4.8] on day 2), and PPI (3 [1-6] vs. 6 [3-7]; p<0.01 on day 1 - 3 [1-6] vs 6 [4-8]; p<0.01 on day 2) than others. PPI and NPi had similar predictive accuracy on day 1 and day 2 for UO. A PPI=1 on day 2 showed a sensitivity of 26 [95% CI 16-38]%, a specificity of 100 [95% CI 89-100]%, a NPV of 39 [95% CI 36-43]% and a PPV of 100% [95% CI 100-100]% to predict UO. On day 2, a total of 6 patients had concomitant PPI=1 and NPi ≤2, while 12 showed NPi>2 and PPI=1; the coefficient of agreement was 0.42. Moreover, NPi and PPI values showed a moderate correlation both on day 1 and day 2. Conclusions: In this study, PPI=1 on day 2 could accurately predict UO in comatose CA patients, with 100% specificity and a higher sensitivity than NPi. The agreement between PPI and NPi values was moderate.