bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRX-527 induced differentiation of HSCs protecting the intestinal epithelium from radiation damage.
Authors: Liu Dongshu , Dong Suhe , Liu Cong , Du Jicong , Wang Sinian , Yu Huijie , Li Wei , Chen Zhongmin , Peng Renjun , Jiang Qisheng , Zou Mengying , Li Fengsheng , Zhang Rong 
Published: May 2022

Abstract
Background: Recently, Toll-like receptors (TLRs) have been extensively studied in radiation damage. However, the inherent defects of high toxicity and low efficacy of most TLR ligands limit their further clinical transformation. CRX-527, as a TLR4 ligand, has rarely been reported to protect against radiation. Methods: In vivo, C57BL/6 mice and TLR4 knockout mice were used to observe the radiation protection of CRX-527 on the hematopoietic system and intestinal system of mice. The proportion and number changes of LSK, MPP, MDSC and macrophages were detected by flow cytometry. The changes of intestinal protein expression were observed by immunohistochemistry, immunofluorescence and Western blot. In vitro, RAW264.7-MODE-K and THP-1-HIEC co-culture systems were performed to observe the proliferation of cells under different irradiation doses in clone formation experiments, as well as the expression of proteins in the co-culture system under the same irradiation doses. Results: CRX-527 improved the survival rate of total body irradiation (TBI) to 100% in wild-type mice but not in TLR4-/- mice. After TBI, hematopoietic system damage was significantly alleviated, and the recovery period was accelerated in CRX-527-treated mice. Moreover, the stimulation of CRX-527 significantly increased the proportion and number of LSK cells and promoted their differentiation into macrophages, activating immune defense. Furthermore, we proposed an immune defense role for hematopoietic differentiation in the protection against intestinal radiation damage, and confirmed that macrophages invaded the intestines through peripheral blood to protect them from radiation damage. Meanwhile, CRX-527 maintained intestinal function and homeostasis, promoted the regeneration of intestinal stem cells, and protected intestinal injury from lethal dose irradiation. Furthermore, CRX-527 protects the hematopoietic and intestinal systems from irradiation through the activation of TLR4-related pathways.Conclusion: CRX-527 induced differentiation of HSCs to maintain the haematopoiesis. The activated macrophage protected the intestinal epithelium from radiation damage through TLR4-related pathways. We demonstrated that CRX-527 was safe and effective in protecting radiation damage in vivo and in vitro.
Genetic polymorphism in BIN1 rather than APOE is associated with poor recognition memory among men without dementia
Authors: Mehta Kanika , Mohebbi Mohammadreza , Pasco Julie , Williams Lana , Walder Ken , Ng Boon , Gupta Veer 
Published: May 2022

Abstract
Although several genetic polymorphisms have been linked with the risk of Alzheimer’s disease, less is known about their impact on cognitive performance among cognitively healthy individuals. Our aim was to investigate the association of the genetic variant, rs744373 in the bridging integrator-1 gene (BIN1), the strongest genetic risk factor for Alzheimer’s disease after the APOE ε4 allele, with different cognitive domains among non-demented older men. Cognitive function was measured using the CogState Brief Battery (CBB), which assessed cognitive performance across four domains: psychomotor function, visual attention, recognition memory/learning and working memory. Linear regression analysis revealed that individuals with the BIN1 risk allele performed poorly on the recognition memory task as compared to those without the risk allele. However, this was in contrast with the individuals who harboured the APOE ε4 risk allele as they displayed better performance on the recognition task in comparison to those without the ε4 risk allele. To the best of our knowledge, this is the first study that demonstrates genetic variation in BIN1 to be a better predictor of recognition memory than APOE, which remains the biggest genetic risk factor for Alzheimer’s disease.
Real-time Motion Management for Robotic Treatment Couch in Proton Therapy System
Authors: Wei Jianghua , Song Yuntao , Ding Kaizhong , Chen Yonghua , Guo Zhoushun , Yuan Hui 
Published: May 2022

Abstract
Background: In proton therapy, robotic couch was used for patient positioning to achieve high precision in cancer treatment. However, no studies have focused on the real-time motion management system (MMS) for the robotic couch. This study was to develop the MMS and verify its feasibility. Methods: A real-time MMS was set up with a robotic couch, an NDI Vega optical tracking system, a Special Measurement Tool (SMT) and the developed software. A Leica laser tracker was adopted to verify the accuracy of the tests in this study. The stability and accuracy of the SMT in NDI Vega system were verified to guarantee the reliability of the results. The coordinate transformation was used to unify the measurement. 83 points inside the treatment volume were applied to verify the accuracy of the MMS, with the comparison of results measured by the laser tracker. The synchronism of the SMT in the MMS and Patient Positioning System (PPS) based on the reciprocating motion along axis z was tested to obtain the maximum time delay. Trajectory tracking was performed with different payloads on the couch top. Results: The Means and Standard Deviation (SD) of the stability of the SMT in NDI tracking system on the axis x, y, z, and Rx, Ry, Rz were 0.005 ± 0.004 mm, 0.013 ± 0.005 mm, 0.014 ± 0.005 mm, 0.010 ± 0.003º, 0.013 ± 0.005º, 0.008 ± 0.007º, respectively. For the MMS, the means ± SD along axis x, y, z were 0.24 ± 0.09 mm (0.12 ± 0.03 mm With Laser Tracker (WLT)), 0.31 ± 0.12 mm (0.10 ± 0.03 mm WLT), 0.18 ± 0.06 mm (0.14 ± 0.05 mm WLT), respectively. The max time delay was about 110 ms, with a 60 s of motion tracking. Trajectories tracking showed the real-time graphical traces of the SMT in NDI Vega and Room Coordinate system, and the combined curves by using the coordinate transformation. Conclusions: A real-time MMS was developed and verified with the accuracy of submillimeter in this study. The results demonstrated the feasibility of potential applications based on real-time motion capturing and monitoring.
The Short versus Long antibiotic course for pleural Infection Management (SLIM) randomised controlled open label trial
Authors: Hassan Maged , Gad-Allah Mohamed , El-Shaarawy Basma , El-Shazly Asmaa M , Daneshvar Cyrus , Sadaka Ahmed 
Published: May 2022

Abstract
Background and objective: The optimal length of antibiotic treatment for pleural infection in adults is not known but a minimum of four weeks is typically recommended. This clinical trial aimed to assess whether shorter antibiotic courses lead to more treatment failures than standard longer courses. Methods: In an open-label randomised controlled trial adult patients with pleural infection who were medically treated and stabilised within 14 days of admission were randomised to either a short antibiotic course (total course 14-21 days) or a long antibiotic course (total course 28-42 days). Patients were excluded if their baseline RAPID score was >4 (high-risk category). The primary outcome was the incidence of treatment failure by 6 weeks post admission. Secondary outcomes were total length of antibiotic treatment, proportion of patients who resumed normal activity levels within 6 weeks post admission, time from discharge to resuming normal activity levels and the incidence of antibiotic-related adverse reactions.Results: Between November 2020 and October 2021 50 patients (35 (70%) males, mean age 46+13.7 years), were recruited to the trial and randomly assigned to the short course group (n=25) or the long course group (n=25) with outcome data available for 24 patients in each study group. Treatment failure occurred in 4 (16.7%) patients in the short course group and 3 (12.5%) patients in the long course group. In the intention-to-treat analysis the odds ratio for treatment failure in the long course group was 0.714 (95% CI 0.142-3.600, p=0.683). The median duration of antibiotic treatment in the short course group was 20.5 [18-22.5] days compared to 34.5 [32-38] days in the long course group (p <0.001). There were no statistically significant differences in the other outcomes.Conclusion: In medically treated adult patients with pleural infection who are stabilised within 14 days of admission and who are not at high risk of mortality a long course of antimicrobial therapy is potentially not superior to a shorter course in terms of risk of treatment failure. These findings need confirmation in a larger multi-centre trial. Trial protocol registration: NCT04615286 (Clinicaltrials.gov), registration date 4th November 2020.
Evolutionary insights on the potential oncogenic impact of long non-coding RNA NNT-AS1 in different types of carcinomas; integrating pathway(s) and clinical outcomes association
Authors: El-Sheikh Nada , Abulsoud Ahmed , Wasfey Eman F. , Hamdy Nadia M. 
Published: May 2022

Abstract
Background Long non-coding RNAs (lncRNAs) are becoming more prevalent in the cancer field arena, with functional roles in both oncogenic and oncosuppressive pathways. Despite their widespread aberrant expression in a range of human malignancies, the biological activities of the ncRNAs majority are unknown. All showed the involvement of the lncRNA nicotinamide nucleotide transhydrogenase antisense RNA 1 (NNT-AS1). Since NNT-AS1 influences cellular proliferation, invasion, migration, apoptosis, and metastasis, this lncRNA appears to be linked to deregulating the normal cellular processes driving malignancy. This was observed in many cancer types such as breast cancer (BC), gastric cancer (GC), colorectal cancer (CRC), epithelial ovarian cancer (EOC), and hepatocellular carcinoma (HCC). Conclusions The current narrative non-systematic review will discuss “the significance of lncRNAs in cancer”, as well as “lncRNAs future potential application(s) as diagnostic or predictive biomarkers”, therefore, comprising an opportunity as treatment target(s). The review will have a special emphasis on lncRNA NNT-AS1.
Comparison of dynamic elasticity between two types of new material plastic table tennis ball: taking DHS D40+ and Nittaku 40+ as an Example
Authors: Wang Zerong , Tian Jiajie , Zeng Yuxuan , Xiao Yi 
Published: May 2022

Abstract
Background: Nittaku 40+ and DHS D40+ are two different brands of new plastic balls, both of which are designated as official match balls and widely used in international events. Due to the change of material and diameter from traditional celluloid table tennis ball to new plastic ball, the hardness and elasticity of the new plastic ball also change accordingly. The purpose of this study is to analyze the difference of the dynamic elasticity between the two brands of new plastic ball, taking Nittaku 40+ ball and DHS D40+ ball as an example.Method: Self-made experiment was designed to generate different levels of initial speed of table tennis ball to test the dynamic elasticity characteristics of DHS D40+ and Nittaku 40+. A high-speed camera was used to collect the trajectory of ball falling and rebound after hitting the table during the experiment, and the Kinovea software was used to process the falling and rebound trajectory and calculate the average speed of falling and rebound process, then accordingly calculate the rebound speed decrement rate. Results: Results showed that the rebound speed and decrement rate of DHS D40+ and Nittaku 40+ both increased with the increase of falling speed, respectively. When falling at slow and medium speeds, there was no significant difference of dynamic elasticity between DHS D40+ and Nittaku 40+. When falling at high speed, there was a significant difference of dynamic elasticity between DHS D40+ and Nittaku 40+.Conclusion: Compared with the DHS D40+, the Nittaku 40+ has bigger hardness and brittleness, and the sphere structure is more uniform, featuring better dynamic elasticity stability and rebound height.
Functional outcomes differ between direct lateral and posterior surgical approaches in total hip arthroplasty during higher demanding activities of daily living
Authors: Tadross Daniel TSF , Chapman Graham J , Redmond Anthony C , Macdonald David , Stone Martin , Lunn David E 
Published: May 2022

Abstract
Background: This study investigated the effect of posterior and direct lateral (modified Hardinge) approach total hip arthroplasty on post-operative hip function compared to healthy, control participants. This study aimed to use a combination of motion analysis, strength measurements and imaging to investigate the effect of surgical approach on post-operative hip function in posterior approach and direct lateral approach cases.Methods: Twenty-eight total hip arthroplasty patients (15 direct lateral approach; 13 posterior approach) and 11 control participants underwent motion capture analysis at two walking speeds (normal and fast walking) and whilst standing from a seated position; hip joint strength and patient reported outcomes were also obtained. Muscle cross-sectional area was measured in a sub-set (N=5) using a novel magnetic resonance imaging sequence incorporating encoding for metal artefact correction.Results: The posterior approach group tended not to achieve full hip extension post-surgically, whereas the direct lateral approach patients tended to extend the hip during the fast walk, similar to control participants. The posterior approach group were weaker in all muscle groups excluding hip abductors, compared to the direct lateral approach patients and control group. In both surgical groups the muscle cross sectional area was reduced in all muscles disrupted during surgery, compared to the contralateral limb. Both surgical groups’ perceived hip function and physical health to be superior compared to the control group.Conclusions: Our findings suggest during sit to stand and fast walking the posterior approach group were functionally poorer and demonstrated weaker hip strength compared to the direct lateral approach group. Furthermore, surgical muscle disruption in our subset of patients is still apparent up to five years post-surgery, as reflected by reduced muscle cross sectional area.
Dissociable Cellular and Genetic Mechanisms of Cortical Thinning at Different Life Stages
Authors: Modabbernia Amirhossein , Vidal-Pineiro Didac , Agartz Ingrid , Andreassen Ole , Ayesa-Arriola Rosa , Bertolino Alessandro , Boomsma D , Bourque Josiane , Breier Alan , Brodaty Henry , Brouwer Rachel , Buitelaar Jan , Canales-Rodriguez Erick , Caseras Xavier , Conrod Patricia , Crespo-Facorro Benedicto , Crivello Fabrice , Crone Eveline , De Zubicaray Greig , Dickie Erin , Dima Danai , Espeseth Thomas , Frenzel Stefan , Fisher Simon , Franke Barbara , Glahn David , Grabe Hans , Grotegerd Dominik , Gruber Oliver , Guerrero-Pedraza Amalia , Gur Raquel , Gur Ruben , Hartman Catharina , Hoekstra Pieter , Pol Hilleke Hulshoff , Jahanshad Neda , Jernigan Terry , Jiang Jiyang , Kalnin Andrew , Kochan Nicole , Mazoyer Bernard , McDonald Brenna , McMahon Katie , Nyberg Lars , Oosterlaan Jaap , Pomarol-Clotet E , Radua Joaquim , Roffman Joshua , Sachdev Perminder , Satterthwaite Theodore , Salvador Raymond , Sarró Salvador , Saykin Andrew , Schumann Gunter , Smoller Jordan , Sommer Iris , Tamnes Christian , Thomopoulos Sophia , Trollor Julian , Ent Dennis van 't , Voineskos Aristotle , Wang Yang , Weber Bernd , Wen Wei , Westlye Lars T. , Whalley Heather , wierenga Lara , Williams Steven , Wittfeld Katharina , Wright Margaret , Thompson Paul , Paus Tomas , Frangou Sophia 
Published: May 2022

Abstract
Mechanisms underpinning neurotypical age-related variations in cortical thickness in the human brain remain insufficiently specified. Here we used cell-specific marker genes, followed by gene ontology and enrichment analyses, to quantify the association between gene-expression levels and inter-regional age-related variations in neurotypical cortical thinning using multicohort neuroimaging data from 14,248 individuals ages 4-89 years. We found that early-life (<20 years), mid-life (20-60 years), and late-life (>60 years) were associated with distinct patterns of association between inter-regional profiles of cortical thickness and expression profiles of markers genes for CA1 and S1 pyramidal cells, astrocytes, and microglia. Gene ontology and enrichment analyses indicated each of the three life-stages was associated with different biological processes and cellular components; these related to synaptic modeling in early life, neurotransmission in mid-life, and neurodegeneration in late-life. These findings provide mechanistic insights on age-related cortical thinning during typical development and ageing.
Genetic structure and north-south decrease of genetic diversity in the Patagonian maqui berry (Aristotelia chilensis [Molina] Stuntz) determined by SSR marker analysis
Authors: Cona Matías , Chávez Alexandra , León-Lobos Pedro , Marín Juan Carlos , Hinrichsen Patricio 
Published: May 2022

Abstract
Maqui (Aristotelia chilensis [Molina] Stuntz) is a small endemic Patagonian tree that is currently being domesticated actively because of its edible berries, which have extremely high polyphenol content and anti-oxidant capability. Maqui can be easily propagated and is an efficient colonizer of deforested habitats to the point of often becoming an invasive species. However, little is known about its population structure and evolutionary history, limiting the design of conservation and domestication strategies. Genetic diversity of 14 populations (183 samples) from throughout the geographic distribution of the species in Chile was assessed with 13 polymorphic microsatellite markers. Samples were assigned to clusters based on Bayesian genetic and spatial structure analyses and the results were used to reconstruct patterns of phylogeographic and demographic history. We found that maqui populations are well-structured, with a dramatic reduction of genetic diversity from north to south, most notably in areas that were covered by ice during the quaternary glaciation. Three main genetic groups were revealed by Structure analysis. In conclusion, a clear pattern of genetic diversity reduction from its northern limit in central Chile to the Patagonian region was revealed, suggesting that an active recolonization process took place during the last few millennia following the last glacial period. These results will help to define the identification of accessions from different regions and contribute to support conservation and domestication initiatives.
Sarcopenia does not affect treatment outcomes in patients with rectal cancer undergoing laparoscopic surgery
Authors: Song Ji Hyeong , Oh Rak Kyun , Lee Jeong Eun , Lee Kyung Ha , Kim Ji Yeon , Kim Jin Soo 
Published: May 2022

Abstract
Purpose: Previous studies have reported that sarcopenia negatively impacts rectal cancer treatment. However, most published studies have analyzed only patients undergoing open surgery, and the association between sarcopenia and clinical outcomes in patients with rectal cancer undergoing laparoscopic surgery remains unclear. This study aimed to evaluate the effect of sarcopenia on the clinical and oncological outcomes of laparoscopic rectal cancer surgery.Methods: Three hundred and one patients undergoing laparoscopic rectal cancer surgery were enrolled between December 2009 and May 2016. Body composition was assessed using computed tomography by measuring the muscle and fat areas at the third lumbar (L3) vertebra. The L3 skeletal muscle area was used to calculate the skeletal muscle index and evaluate sarcopenia.Results: Sarcopenia was identified in 72/301 (23.9%) patients. Patients with sarcopenia had decreased skeletal muscle area (P < 0.001). However, there were no significant differences in visceral fat, subcutaneous fat, and body mass index. The time to tolerable soft diet and length of hospital stay were shorter in sarcopenia patients. The time to first flatus, postoperative complications, and overall and disease-free survival rates were not significantly different between those with and without sarcopenia. High American Society of Anesthesiologist classification (≥3; P = 0.014) and abdominoperineal resection (P = 0.003) were identified as risk factors for postoperative complications.Conclusion: Sarcopenia does not negatively affect the functional recovery, postoperative complications, or survival after laparoscopic rectal cancer surgery. Further studies are necessary to validate that laparoscopic surgery could eliminate the negative impact of sarcopenia in patients with rectal cancer.
ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cells
Authors: Suzuki Soichiro , Ando Fumiaki , Kitagawa Sae , Hara Yu , Fujiki Tamami , Mandai Shintaro , Susa Koichiro , Mori Takayasu , Sohara Eisei , Rai Tatemitsu , Uchida Shinichi 
Published: May 2022

Abstract
Signaling through cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) promotes endothelial barrier function to prevent plasma leakage induced by inflammatory mediators. The discovery of novel PKA substrates in endothelial cells has increased our understanding of the molecular mechanisms involved in vessel maturation. In this study, we evaluated a cAMP inducer, forskolin, and a phospho-PKA substrate antibody to identify ZNF185 as a PKA substrate. ZNF185 interacted with PKA and a deletion mutant of ZNF185, which lacks the PKA-interacting domain, was not phosphorylated by PKA. In addition to PKA, ZNF185 colocalized with F-actin in endothelial cells. Both phospho-ZNF185 and F-actin accumulated in the plasma membrane region in response to forskolin to stabilize the cortical actin structure. By contrast, ZNF185 knockdown disrupted actin filaments and promoted stress fiber formation without inflammatory mediators. Constitutive activation of RhoA was induced by ZNF185 knockdown, which resulted in forskolin-resistant endothelial barrier dysfunction. ZNF185 was essential for cAMP/PKA/RhoA signaling for the suppression of endothelial hyperpermeability. Thrombin protease was used as a positive control to assemble stress fibers via RhoA activation. Unexpectedly, ZNF185 was cleaved by thrombin, resulting in an N-terminal actin-targeting domain and a C-terminal PKA-interacting domain. Irreversible dysfunction of ZNF185 protein potentially causes RhoA-dependent stress fiber formation by thrombin.
Learner Performance-based Behavior Optimization Algorithm: A Functional Case Study
Authors: Shahad  , Rashid Tarik A. 
Published: May 2022

Abstract
A novel algorithm called learner performance-based behavior algorithm(LPB) was proposed for single and multi-objective by Chnoor M. Rahmanand Tarik A. Rashid in 2021. LPB proved its ability to deal with complex optimizationproblems compared to the dragonfly algorithm (DA), genetic algorithm(GA), and particle swarm optimization (PSO). This paper presents and explainsthe implementation of the LPB algorithm, and it applies it as a model in a casestudy to maximize a fitness function. As a result, the LPB algorithm is successfullyimproved the initial population and achieved the optimal solution.