bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transforming Communication in Health Care via Information Technology: Implementation of a free telephone directory app
Authors: Hania A , Barkat R , Nolan R , Buggy E , Eochagain A Ni 
Published: April 2022

Abstract
Background:Timely communication is key to patient safety and management in health care. Communication failure and delays in the transfer of information between healthcare workers can result in avoidable errors. We measured the effect of the implementation of a free telephone directory app for hospital staff to connect efficiently within the hospital.Methods:We implemented a free telephone directory app for smartphones which is available to all hospital staff that provides immediate access to all the internal phone lines and bleeps without calling switch. An audit of telephone calls made to switch before and after the implementation of the app was then performed.Results:Number of total calls to switch board in a 24hour period reduced by 10.9% (2,248 to 2,002 calls) following implementation of the app. The proportion of calls to switch which were from an internal line with staff requesting the number of an internal line or bleep was also reduced from 29.9% (n=672) to 24.6% (n=492), p-value <0.001.Conclusion:The use of a hospital phone directory app with mobile technology is a cost-effective measure that can benefit the hospital by improving efficiency in hospital switchboards, enhance communication and productivity for healthcare workers which can help improve patient care.
Immune characteristics and genetic markers of esophageal cancer by single-cell analysis: Implications for immunotherapy
Authors: Xu Bing , Chen Hui , Tseng I Hsuan , Ai Dashan , Fang Xumeng , Shen Jingyi , Liu Qi , Chen Yun , Sun Xinchen , Ge Xiaolin , Zhu Hongcheng , Zhao Kuaile 
Published: April 2022

Abstract
Background: Esophageal cancer (EC) is one of the most common cancers worldwide. The prognosis for patients with the same stage of EC can vary substantially. Progress of single-cell analysis technology has furthered the understanding of tumor heterogeneity. This paper aimed to apply single-cell analysis to explore the characteristics of the tumor environment of EC and provide inspiration for personalized treatment.Methods: The latest gene expression data and clinical follow-up information of single-cell sequencing results of EC samples were downloaded using from The Cancer Genome Atlas (TCGA) Genomic Data Commons (GDC) Application Programming Interface (API). A differential gene function analysis of the immune infiltration signature agents in the tumor microenvironment was undertaken using bioinformatics analytic methods, and potential molecular targets were sought.Results: We identified specific cell subsets in the EC and paracancerous samples, including panel cells, natural killer (NK) cells, exhausted CD8+ T cells, CD8+ memory T (Tcm) cells, and effector memory T (Tem) cells, including B cell enrichment in the cancer samples. Differences were detected between B cells and monocytes in Stage II and Stage III tumors, and these differences may have related to RNA transcription and degradation. The CXCL8 protein was identified as a valid potential prognostic marker.Conclusions: Cell groups with homogenous cell surface markers exhibit intercellular variations that exert a considerable effect on cell function. Our study will contribute to the understanding of the tumor immune microenvironment (TME) and cellular heterogeneity in EC patients and serve as a valuable resource for in-depth exploration of the pathogenesis of EC and the identification of potential therapeutic targets in the future.
Analysis of the circumferential bulging capacity and uniformity under different TEC layout and the roll structure in the ETCR
Authors: Xu Zhiqiang , hai yang , Yang Tingsong , Zhou Haonan , Du Fengshan 
Published: April 2022

Abstract
Electronic temperature control roll (ETCR) technology is a new roll profile control technology based on the principle of the semiconductor cooling and heating effect. The thermoelectric cooler (TEC) is the core component for controlling roll profiles in this technology. The TEC layout and the roll structure can affect the bulging ability of the roll, the bulging uniformity and the uniformity of the roll temperature field. To research the influence of the TEC layout and the roll structure on these factors, a circumferential finite element model of ETCR is established by the finite element software MSC.MARC, and is verified on the experimental platform of ETCR. After analysis, it was found that increasing the roll diameter or decreasing the diameter of the roll inner hole can increase the bulging ability of the roll, improve the bulging uniformity and weaken the temperature ratchet. Increasing the temperature of TEC or decreasing the single piece influence angle can increase the bulging ability of the roll and improve the temperature ratchet, while the effect of changing the single piece influence angle on the bulging uniformity is larger than that of the temperature of TEC. In view of the difficulty of circumferential uniform bulging when the single piece influence angle is large, a scheme of adding a metal layer of high thermal conductivity material in the roll inner hole is proposed. The results showed that the copper layer has little influence on the bulging ability of the roll and can reduce the circumferential bulging difference.
The Significance of Chaos/Complexity Theory in Maltese as a Second Language Acquisition
Authors: Żammit Jacqueline 
Published: April 2022

Abstract
Despite extensive research in second language acquisition (SLA), we are still a long way from understanding what exactly happens in the adult’s mind while learning a second language (L2). This study explores whether a learning pattern could be established over time in 35 adults learning Maltese as a second language (ML2), especially with respect to Maltese verbs. This research is driven by chaos/complexity theory. It focuses on the non-linear learning curve, the origins of the butterfly effect and fractal patterns of learning. It describes how learning is unpredictable, chaotic, dynamic and complex. A longitudinal research system and a mixed-method approach focussed on methodological triangulation were used in this research. Structured Timed Grammaticality Judgment Tests, verb conjugation tasks, reflected journals and interviews were used to investigate the learning curve over a period of 15 months. According to the results, all participants indicated a non-linear learning pattern and confirm the characteristics of Chaos/Complexity Theory.
Comparison of various neonatal transport scoring methods in predicting mortality risk for full-term out-born infants
Authors: Qu Wenwen , Shen Yanhua , Qi Yujie , Jiang Min , Zheng Xu , Zhang Jinjing , Wu Dan , He Wenwen , Geng Wenjing , Hei Mingyan 
Published: April 2022

Abstract
The transport of critically ill newborns makes it possible for many high-risk neonates to survive. Relatively little is known about death factors and the risk assessment of full-term newborns. We compared the effectiveness of neonatal transport scoring methods to predict the mortality risk of full-term out-born infants within the first week after transportation. A retrospective cohort study was conducted in the Neonatal Intensive Care Unit (NICU) of Beijing Children’s Hospital from January 2019 to December 2020. All patients were full-term out-born neonates admitted by our transport team. Each patient was assessed by the following scores: the Transport Risk Index of Physiologic Stability (TRIPS) score; the Mortality Index for Neonatal Transportation (MINT) score; the Transport Related Mortality Score (TREMS); and the Neonatal Critical Illness Score (NCIS). Receiver operating characteristic (ROC) and decision curve analysis (DCA) in the R environment were used to test and compare the effectiveness and clinical utility of these scores in predicting the mortality risk of full-term infants within the 1st week after admission. In total, 368 full-term infants were included (368/770, 47.8% of all transported infants). Within the 1st week after admission, five infants (1.36%, 5/368) died under advanced life support and full treatment, while 24 infants (6.52%, 24/368) died soon after they were discharged against medical advice. The AUCs of the MINT, TRIPS, TREMS and NCIS in the prediction of mortality were 0.822, 0.827, 0.643 and 0.731, respectively (all p<0.05). However, the clinical net benefit was far superior for the MINT and TRIPS scores than for the NCIS and TREMS scores.Conclusions: TRIPS and MINT scores might be more suitable for predicting mortality in full-term out-born neonates in the NICU within the 1st week after transportation.
Immunolocalization of zinc transporters and metallothioneins reveals links to microvascular morphology and functions
Authors: Tran Hai Bac , Jakobczak Rachel , Abdo Adrian , Asare Patrick , Reynolds Paul , Beltrame John , Hodge Sandra , Zalewski Peter 
Published: April 2022

Abstract
Zinc homeostasis is vital to immune and other organ system functions, yet over a quarter of the world’s population are zinc deficient. Abnormal zinc transport or storage protein expression has been linked to diseases, such as cancer and chronic obstructive pulmonary disorder. Although recent studies indicate a role for zinc regulation in vascular functions and diseases, detailed knowledge of the mechanisms involved remain unknown. This study aimed to assess protein expression and localization of zinc transporters of the SLC39A/ZIP family (ZIPs) and metallothioneins (MTs) in human subcutaneous microvessels, and to relate them to morphologic features and expression of function-related molecules in the microvasculature. Microvessels in paraffin biopsies of subcutaneous adipose tissues from 14 patients undergoing hernia reconstruction surgery were analysed for 9 ZIPs and 3 MT proteins by MQCM (multifluorescence quantitative confocal microscopy). Zinc regulation proteins detected in human microvasculature included ZIP1, ZIP2, ZIP8, ZIP10, ZIP12, ZIP14, and MT1-3, which showed differential localization among endothelial and smooth muscle cells. ZIP1, ZIP2, ZIP12 and MT3 showed significantly (p < 0.05) increased immunoreactivities, in association with increased microvascular muscularization, and upregulated ET-1, α-SMA and the active form of p38 MAPK (Thr180/Tyr182 phosphorylated, p38 MAPK-P). These findings support roles of the zinc regulation system in microvascular physiology and diseases.
Interrelation of T-cell Cytokines and Autoantibodies in Lupus Nephritis: A Cross-sectional Study
Authors: Alduraibi Fatima , Sullivan Kathryn , Chatham W.Winn , Hsu Hui-Chen , Mountz John D. 
Published: April 2022

Abstract
Objective: To determine if circulating autoantibodies (autoAbs) and T-helper cell cytokines correlate with a different class of lupus nephritis (LN), including class III/IV, compared to class V and other manifestations of systemic lupus erythematosus (SLE). Methods: All patients (N=62) met the SLE classification criteria of the American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) or Systemic Lupus International Collaborating Clinics (SLICC) classification criteria. Demographic, clinical data, and serologic manifestations included anti-DNA, anti-Smith (Anti-Sm), C3, and C4 were included. Plasma levels of interferon gamma (IFNɣ), interleukin 17 (IL-17), interleukin 10 (IL-10) isotype-specific (IgG) anti-DNA, anti-Ro/SSA (SSA), and anti-Sm were measured using enzyme-linked immunosorbent assay (ELISA).Results: The most common manifestations by history were arthritis in 61% (N=38), photosensitivity rash in 53% (N=33), LN in 44% (N=27), and oral/nasal ulcer in 31% (N=19) SLE patients. AutoAbs (anti-DNA, anti-SSA, and anti-Sm), IFNɣ, and IL-17, but not IL-10, were significantly elevated in SLE patients compared to healthy controls. There were elevated plasma levels of anti-DNA (p = 0.0101) and anti-Sm (p = 0.0499) in patients with a history of LN compared to patients without LN. In contrast, plasma levels of anti-Sm were decreased in patients who had a history of acute mucocutaneous manifestations, including photosensitivity rash and/or malar rash (p = 0.0152). Among the three cytokines that were analyzed, IL-10 was significantly elevated in patients with a history of LN compared to patients without LN (p = 0.0216). IL-17 was positively correlated with anti-SSA (p = 0.0130) and was significantly higher in patients with discoid rash (p = 0.0238) and history of class V LN (p = 0.0055). IFNɣ was positively correlated with anti-DNA (p = 0.0355) and anti-SSA (p = 0.0402). Conclusion: This cross-sectional study supports the role of different T-helper cell cytokines that may be associated with the development of different autoAbs in influencing the diversity of SLE clinical manifestations. The results suggests that elevated IFNɣ and IL-17 are more generalized features in SLE patients. In contrast, higher levels of IL-10 were observed in patients with a history of LN. This provide insights into the pathogenic mechanisms of LN that can help guide future diagnosis and therapies.
REIIBP promotes TLR7-BTK-IL6 pro-inflammatory response through histone H3 lysine methylation in t(4;14) myelomagenesis
Authors: Chong Phyllis S.Y. , Chooi Jing Yuan , Lim Sze Lynn Julia , Chung Tae-Hoon , Toh Sabrina Hui Min , Balan Kalpnaa , Azaman Muhamad Irfan Bin , Wu Zhengwei , Wee-Joo Chng 
Published: April 2022

Abstract
Background
Recurrent chromosomal translocations are central to the pathogenesis of Multiple Myeloma (MM), and the t(4;14) translocation is an adverse prognostic factor found in approximately 15% of MM patients. REIIBP is a t(4;14)-deregulated histone methyltransferase arising from alternative promoter usage within the MMSET locus. The role of this short isoform in t(4;14) myelomagenesis is poorly understood.
Methods
RPMI8266 was transfected with REIIBP vector to create stable isogenic cells using puromycin selection, and the oncogenic and histone methyltransferase activities were evaluated by RT-qPCR, immunoblotting, viability and colony-forming assays. Microarray and ChIP-sequencing were used to identify REIIBP targets, followed by genetic and biochemical inhibition to determine the underlying mechanisms in vitro and in vivo. Clinical relevance was validated with CoMMpass dataset.
Results
Despite sharing identical sequence with the C-terminus of MMSET, we found that REIIBP displayed distinct expression pattern with other MMSET breakpoint variants and gene products of the t(4;14) locus. Additionally, the expression of REIIBP can be regulated through microRNAs by another histone methyltransferase, EZH2 in a Dicer-dependent manner. Stable overexpression of REIIBP in the t(4;14)-negative RPMI8226 promoted oncogenic phenotypes and predominantly catalyzes the repressive H3K27me3 and active H3K4me3 histone modifications. Identification of differentially-regulated genes through microarray and correlation with H3K4me3/H3K27me3 ChIP-sequencing revealed that Toll-like receptor 7 (TLR7) and Bruton’s tyrosine kinase (BTK) are targets of REIIBP. Importantly, this led to a B-cell receptor (BCR)-independent activation of BTK and NF-ĸB effector signaling in t(4;14) myeloma cells. As myeloma cells do not express BCR, the re-activation of BTK signaling by TLR7 was functionally validated, conferring bortezomib resistance through the dysregulation of pro-inflammatory cytokine expression such as IL-6 and IL-27. Furthermore, comparison of BTK expression and dependency scores across lineages revealed that BTK is an important target in multiple myeloma, and correlated with REIIBP expression in the CoMMpass dataset. t(4;14)-translocated cells were amenable to BTK targeting using Ibrutinib, improving bortezomib response in myeloma cells and mice engrafted with RPMI8226-REIIBP tumors.
Conclusion
Altogether, our results provided a rationale for the blockade of REIIBP in t(4;14) cells through combining proteasome and BTK inhibitors as a therapeutic strategy for further clinical evaluation.
Resection of scar tissue in rats with spinal cord injury can promote the expression of βⅢ-tubulin in the injured area
Authors: Liu Baoguo , Liu Sumei , Sun Dejun 
Published: April 2022

Abstract
Background The scar tissue formed in the injured area after spinal cord injury is one of the reasons that hinder nerve regeneration and tissue repair. In previous studies, the researchers tried to prevent the formation of scar tissue to promote nerve regeneration and tissue repair, but it ran counter to the their desire, which also reflected the favorable side of scar tissue in the formation process. Further studies confirmed that scar formation of tissue is a dynamic process of hindering and promoting, nerve regeneration and tissue repair. By grasping the dual action of glial scar, understanding the underlying mechanism of its diversity is the key to break through the regeneration of nerve function after spinal cord injury.Results Based on the rat models of the transection injury of the T8-T9 spinal cord, we selected several different key time points to resect the scar tissue formed after spinal cord injury and we found that the resection of scar tissue promoted the recovery of motor function and the expression of βⅢ-tubulin in the injured area, especially significant after scar excision in the second week after spinal cord injury. Further studies have shown that the mechanism of recovery might be a combination of promoting nerve axons regeneration, promoting nerve signal transmission, forming functional blood vessels, protecting neurons survival, and reducing scarring, etc. Meanwhile, we found that Tubb3 and Tubb6 genes were significantly different in the tissue before and after early scar resection.Conclusions The motor function of the rats with spinal cord injury after scar resection was improved, and it could be seen that the expression of βⅢ-tubulin increased in the re-formed scars. The Tubb3, Tubb6 genes expression and other neural regeneration pathways were significantly different in the tissue before and after early resection. It was proved that excision of scar tissue promoted nerve regeneration.
Analysis of clinical features and prognosis of HPV-negative endocervical adenocarcinoma
Authors: Ju Shuang , Zhang Junhua , Liu Xiaoli , Liu Lu , Sun Wenxiong , Mu Wendi , Guo Lingyu , Sun Yu , Han Sai , Zhang Youzhong 
Published: April 2022

Abstract
Objectives: To analyze the differences in clinical features and prognosis of HPV-negative and HPV-positive endocervical adenocarcinoma (ECA).Methods: The database of Qilu Hospital was searched for women with HPV testing who underwent primary surgery for ECA between January 2014 and December 2020. The clinical features of the two groups were compared, and the prognosis was evaluated by Kaplan–Meier survival curves. Moreover, immunohistochemical characteristics were reviewed in combination with literatures.Results: Among the 198 enrolled cases of ECA, 62 cases (31.31%) were HPV-negative ECA. Compared with HPV-positive ECA, HPV-negative ECA was more significantly associated with age, clinical symptoms, cervical cytology, CA125, FIGO stage, histologic subtypes, tumor diameter (<3 cm), deep stromal invasion (DSI), lymphovascular space invasion (LVSI), ovarian metastasis and intermediate-risk factors (all p<0.05). However, no significant differences were observed in the pregnancy and parturition times, CA19-9 level, CEA level or lymph node metastasis between the two groups (all p≥05). The OS of patients with HPV-negative ECA was poorer than that of those with HPV-positive ECA (p=0.00). Multivariable analysis showed that HPV-negative status, lymph node metastasis and DSI were significantly associated with the OS of ECA.Conclusions: HPV-negative ECA is characterized by older age, larger tumor, higher stage, susceptibility to LVSI, insensitivity to radiotherapy and chemotherapy and poor prognosis. Furthermore, lymph node metastasis and DSI can be used as predictors of prognosis in ECA. The diagnosis of HPV-negative ECA requires consideration of cervical cytology, colposcopy screening and clinical symptoms. And, biopsy plus immunohistochemistry can improve accuracy of diagnosis.
Cytotoxicity and anti-biofilm activities of biogenic cadmium nanoparticles and cadmium nitrate: a preliminary study
Authors: Adeli-Sardou Mahboubeh , Shakibaie Mojtaba , Forootanfar Hamid , Jabari-Morouei Fereshteh , Riahi-Madvar Soudabe , Ghafari-Shahrbabaki Sima-Sadat , Mehrabani Mitra 
Published: April 2022

Abstract
Cadmium nanoparticles (Cd NPs) were prepared using Artemisia persica extract and microwave irradiation in this research. Cd NPs and Cd (NO3)2 were evaluated for their cytotoxicity, antibacterial, anti-biofilm, and biocompatibility. It was found that Cd NPs (11.2–18.6 nm) had a lower cytotoxic effect than Cd (NO3)2 against both HepG2 and HUVEC cells. The antibacterial activity of Cd NPs against clinically isolated Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa was above 2560 μg mL-1. The Cd NPs (640 μg mL-1) decreased the biofilm formation by S. aureus, P. mirabilis, and P. aeruginosa to 24.6%, 31.6%, and 26.4%, respectively. Adding Cd NPs (100 μg/disc) to commercial antibiotic discs increased the antibacterial activity of vancomycin, gentamicin, tetracycline, streptomycin, meropenem, and kanamycin against Methicillin-resistant S. aureus, significantly. The Cd NPs (640 μg mL-1) exhibited low hemolysis activity. More research should be conducted to understand the related cytotoxicity and antibacterial mechanisms of Cd NPs.