bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Lead on Enzyme Activity in the Liver of Tilapia (Oreochromis niloticus)
Authors: Karimov Erkin Bakhtiyerovich , Halley Grayson , Li Fen , Jiazhang Chen 
Published: June 2022

Abstract
Worldwide heavy metals belong to one of the most important groups of pollutants. Most of them including cadmium, copper, lead, and zinc are considered extremely dangerous in the eco-toxicological aspect. Recently, the use of biological markers or biomarkers measured at the molecular or cellular level has been proposed as a sensitive tool to evaluate oxidative stress in fish. The study aimed to investigate the potential of lead (Pb) in water to affect growth performance, weight gain, feed utilization, and its potential to induce oxidative stress and enzyme activity disruptions. Five treatments were designed including a control and Pb contaminated water at 0.01, 0.05, 0.25 and 1.25 mg/L concentrations. Results from the present study showed that fish exposed to Pb contaminated water at the highest tested concentration resulted in significantly high (P < 0.05) lipid peroxidation levels in tilapia when compared to all other groups and the control. Furthermore, a contaminant in water resulted in a significant increase of glutathione (GSH) activity in the liver of fish compared to control. However, no significant changes in catalase (CAT), glutathione-S-transferase (GST), and superoxide dismutase (SOD) activities were observed when compared among groups and with the control.
Dynamically Stable and Amplified Circularly Polarized Excimer Emission (CPEE) of Achiral Pyrene-Based Dye Regulated by Solvation of Chiral Co-assembly Process
Authors: Zhang Yuxia , Li Hang , Geng Zhongxing , Zheng Wenhua , Quan Yiwu , Cheng Yixiang 
Published: June 2022

Abstract
Chiral supramolecular assembly has been assigned to be one of the most favorable strategies for the development of excellent circularly polarized luminescent (CPL)-active materials. Herein, we report our study of an achiral boron-containing pyrene (Py)-based chromophore (PyBO) as a circularly polarized excimer emission (CPEE) dye induced by chiral co-assemblies containing chiral binaphthyl-based enantiomers (R/S-M). Chiral co-assembly R/S-M-(PyBO)4 fresh film spin-coated from toluene solution can exhibit orderly nanofibers and strong green CPEE (λem = 512 nm, gem = ± 0.45, ΦFL = 51.2 %) resulting from an achiral PyBO excimer. In contrast, only a very weak blue CPL was observed (λem = 461 nm, gem = ± 0.0125, ΦFL = 19.0 %) after 187 h due to PyBO monomer emission as spherulite growth. Interestingly, this kind of chiral co-assembly R-M-(PyBO)4-T film from tetrahydrofuran (THF) solution retains uniform morphology and affords the most stable and strongest CPEE performance (λem = 512 nm, gem = + 0.62, ΦFL = 53.3 %) after 10 days.
High and low value care recommended and undertaken prior to knee or hip arthroplasty: A survey study
Authors: Mills Kathryn , Brewster Anne-Marie , Hackett Danella , Cheung Chris , Solomon Michael , Naylor Justine 
Published: June 2022

Abstract
Introduction: Interventions for osteoarthritis (OA) vary widely in value. Currently, appointment attendance, audits and practitioner surveys are used to determine which interventions are recommended prior to arthroplasty. The patient perspective is missing from this evidence base. Objective: To describe the prevalence of high and low value care being recommended and undertaken by individuals awaiting OA-related lower limb arthroplasty. To examine sociodemographic or disease-related variables associated with being recommended different levels of care. Methods: A cross-sectional survey of 339 individuals was conducted in metropolitan and regional hospitals and surgeon consultation rooms. Along with demographic and disease-related questions, respondents were asked what intervention they were recommended by healthcare practitioners, or other sources of information, and what they had undertaken within two years prior to hip or knee arthroplasty. Results: Simple analgesics were most frequently recommended (68% [62.9 to 73.1). 24.8% [20.2 to 29.7] of respondents were recommended high value care only. 75.2% [70.2 to 79.7] of respondents were recommended at least one low value intervention. Respondents awaiting hip arthroplasty, living regionally and without private health insurance had greater odds of recommended rather than core interventions being advised. Conclusion: The prevalence of recommendations and uptake of high value care appears to be increasing. In most cases they are combined with recommendations for low value care. From a patient’s perspective, disease-related and sociodemographic variables influence the level of care recommended.
Sea Surface Temperature Predictability at the interface between oceanographic modelling and machine learning
Authors: Boschetti Fabio , Feng Ming , Hartog Jason R. , Hobday Alistair J. , Zhang Xuebin 
Published: June 2022

Abstract
Ensemble models, statistical analysis and machine learning (ML) can be used to predict novel conditions in a rapidly changing ocean. Traditionally, ML has been understood as a purely data-driven approach. Recently, success has been reported in training ML on both observational and model data to forecast Sea Surface Temperature (SST) anomalies. Here we use ML trained only on climate model simulations to predict regional SST variations, thereby suggesting a novel role for ML as an ensemble model interpolator. We propose a measure of the predictability provided by different ML implementations as well as by standard time series analysis methods. Weighting each forecast by this predictability measure computed on model data only, provides a significant improvement in forecast skill. We demonstrate the performance of this approach for regions around Australia, the Nino3.4 region (central-eastern equatorial Pacific) and in the eastern equatorial Pacific and discuss the implications for SST predictability as a function of geographical location, area size, seasonality, proximity to the coast and model data quality.
Association between oral health behavior and multiple chronic diseases among middle-aged and older adults in Beijing, China
Authors: Guo Dan , Shi Zhenyu , Luo Yanan , Ding Ruoxi , He Ping 
Published: June 2022

Abstract
Objectives
To evaluate the association between oral health behavior and multiple chronic diseases among middle-aged and older adults.
Methods
Data from the Beijing Health Service Survey were used to estimate the association between oral hygiene behavior and risk of multiple chronic diseases using multivariate logistic models(N = 18,158).
Results
The risk of overall chronic diseases (OR = 1.27, 95% CI:1.18 ~ 1.37), cardiovascular diseases (CVD, OR = 1.30, 95% CI:1.21 ~ 1.39) and endocrine or nutritional metabolic disorders (OR = 1.11, 95% CI:1.01 ~ 1.22) was higher in those who with poor oral health behavior. There was no significant correlation between oral health behavior and the risk of diseases of the musculoskeletal, respiratory, digestive, and genitourine systems.
Conclusions
Poor oral hygiene practices were associated with a higher risk of overall chronic diseases, CVD and diabetes mellitus (DM) in middle-aged and older people. These findings motivate further studies to evaluate whether improved oral health behavior may prevent the incidence of chronic diseases.
Noname manuscript No. (will be inserted by the editor)
Nonlinear Normal Modes based Study of Synchronization in Delay Coupled Limit Cycle Oscillators.
Authors: Pandey Manoj , M Govind 
Published: June 2022

Abstract
Nonlinear normal modes (NNMs), a powerful analytical tool for studying nonlinear systems is used here to study synchronization dynamics in delay coupled oscillators, which exhibit limit cycle oscillations (LCO) and hysteresis when uncoupled. A method described by Shaw and Pierre is used to arrive at the NNMs which are found to be two dimensional invariant manifolds containing closed curves corresponding to either the in-phase or out-of-phase synchronized oscillations for both delayed and non-delayed, reactively or dissipatively coupled systems. Motion initiated on these NNMs, is found to evolve strictly on them, settling onto a stable orbit if existent or on to a quasi-periodic orbit lying between the two manifolds in the absence of one. The NNMs are used to decouple the governing equations of the system, giving the in-phase and out-of-phase synchronization frequencies directly. Parametric study in terms of strength of coupling, frequency detuning and delay time is performed using the averaged equations of the system, aided by continuation software AUTO, which also reveals the bifurcations in the system. These are verified using NNM, direct numerical integration and shooting method based Floquent analysis of the original system and are found to match well. The results obtained here agree with prior work in literature, at the same time extending them to hitherto unexplored parameter range and provide a new perspective on the same from the point of view of NNMs. Hence this study demonstrates the utility of NNMs for the study of synchronization dynamics in both delayed and non delay mutually coupled LCOs and can be useful for studying larger arrays of the same.Mathematics Subject Classification (2020) 70K75 · 34D06 · 34K24
Ghrelin regulates crosstalk between apoptosis, necroptosis and autophagy programmed cell death pathways in the hippocampal neurons of amyloid-β 1–42-induced rat model of Alzheimer’s disease
Authors: Naseri Faezeh , Sirati-Sabet Majid , Sarlaki Fatemeh , Keimasi Mohammad , Mokarram Poneh , Siri Morvarid , Ghasemi Rasoul , Shahsavari Zahra , Goshadrou Fatemeh 
Published: June 2022

Abstract
Alzheimer's disease (AD) is a common progressive and irreversible neurodegenerative disorder. Neuronal loss in the brain is one of the important characteristic features of AD, which is along with memory and cognitive dysfunction. Activation of programmed cell deaths, especially apoptosis and necroptosis, and autophagy failure plays a critical role in the pathogenesis of AD. Ghrelin, an appetite-related hormone, exerts neuroprotective effects via the stimulation of growth hormone secretagogue receptor type 1a in the central nervous system. In this study, rats' cognitive impairment was induced by intra-hippocampal Cornu Ammonis 3 administration of amyloid-beta 1–42 (Aβ 1–42). Then animals were treated with ghrelin 80 µg/kg intraperitoneally for 10 consecutive days. The Morris water maze and passive avoidance learning test were used to evaluate of effect of ghrelin on learning and memory performance. After that, histopathological study on rat hippocampus was done, and the expression of pro-apoptotic protein Bax, anti-apoptotic protein Bcl-2, necroptotic proteins RIP1K and RIP3K and autophagic marker Beclin-1 were assessed using western blotting method. Our findings showed that ghrelin improves memory impairment in Aβ 1–42-induced rats and reduces Bax, RIP1K, and RIP3K expressions and also Bax/Bcl-2 ratio, as well as Beclin-1 expressions. In conclusion, our study suggests that ghrelin can provide neuroprotection via apoptosis and necroptosis inhibition and autophagy promotion and also crosstalk regulation between three cell death pathways in Aβ 1–42-induced model of Alzheimer's disease.
Non-destructive assessment of equivalent umami concentrations in salmon using hyperspectral imaging technology combined with multivariate algorithms
Authors: Sun Zongbao , Pan Haodong , Zuo Min , Li Junkui , Liang Liming , Ho Chi-Tang , Zou Xiaobo 
Published: June 2022

Abstract

This study utilized equivalent umami concentrations (EUC) to characterize umami intensity in salmon with different freeze-thaw times. A rapid and non-destructive method was established to determine EUC values in salmon which is based on hyperspectral imaging (HSI) system combined with multiple characteristic variable screening methods. The established CARS-PLS model showed greater advantages in correlating the reference values of spectral data with EUC in salmon with Rc of 0.9012, Rp of 0.9009, RMSECV of 0.82, and RMSEP of 0.88. The model was employed pixel-wise to visualize the distribution of EUC with different freeze-thaw times, which demonstrated the reduction of EUC value with the increasing of freeze-thaw times. Therefore, this reseearch showed hyperspectral imaging (HSI) system combined with chemometrics possesses a substantial capability to predict and visualize the EUC of salmon, which would provide an intuitive understanding of salmon quality prediction and detection.
The matrilineal ancestry of Nepali populations
Authors: Basnet Rajdip , Rai Niraj , Tamang Rakesh , Awasthi Nagendra , Pradhan Isha , Parajuli Pawan , Kashyap Deepak , Reddy Alla Govardhan , Chaubey Gyaneshwer , Manandhar Krishna Das , Shrestha Tilak Ram , Thangaraj Kumarasamy 
Published: June 2022

Abstract
The Tibetan plateau and high mountain ranges of Nepal are one of the challenging geographical regions inhabited by modern humans. While much of the ethnographic and population-based genetic studies were carried out to investigate the Tibetan and Sherpa highlanders, little is known about the demographic processes that enabled the colonization of the hilly areas of Nepal. Thus, the present study aimed to investigate the past demographic events that shaped the extant Nepalese genetic diversity using mitochondrial DNA (mtDNA) variations from ethnic Nepalese groups. We have analyzed mtDNA sequences from 999 Nepalese samples and compared the data with 38,622 published mtDNA sequences from the rest of the world. Our analysis revealed that the genomic landscapes of prehistoric Himalayan settlers of Nepal were similar to the low altitude extant Nepalese (LAN), especially to that of Newar and Magar population group; but differ from contemporary high-altitude Sherpas. LAN might have derived their East Eurasian ancestry mainly from low altitude Tibeto-Burmans, who might have migrated from East Asia and assimilated across the Eastern Himalayas extended from Eastern Nepal to North-East of India, Bhutan, the Tibet and Northern Myanmar. We also identified a clear genetic sub-structure across different ethnic groups of Nepal based on mtDNA haplogroups and ectodysplasin-A receptor (EDAR) gene. Our comprehensive high-resolution mtDNA-based genetic study of Tibeto-Burman communities reconstructs the maternal origins of prehistoric Himalayan populations and sheds light on migration events that have brought most of the East Eurasian ancestry to the present-day Nepalese population.
Aging reprogramming liver endothelial zonation triggers steatohepatitis by inactivating pericentral endothelium-derived C-kit
Authors: Duan Juanli , Liu Jingjing , Ruan Bai , Ding Jian , Fang Zhiqiang , Xu Hao , Song Ping , Xu Chen , Li Zhiwen , Du Wei , Wang Lin 
Published: June 2022

Abstract
Aging leads to systemic metabolic disorders including nonalcoholic steatohepatitis (NASH). Here, we showed that aging-induced liver sinusoidal endothelial cell (LSEC) senescence accelerated liver sinusoid capillarization and promoted steatohepatitis by reprogramming liver endothelial zonation and inactivating pericentral endothelium-derived C-kit. Abrogation of endothelial C-kit triggered cellular senescence which disturbed LSEC homeostasis. During diet-induced NASH development, C-kit deletion aggravated hepatic steatosis and exacerbated NASH-associated fibrosis and inflammation. Mechanistically, CXCR4/SDF-1 signaling was inhibited by C-kit. Blocking CXCR4/SDF-1 signaling by AMD3100 abolished LSEC-macrophage crosstalk and recovered the aggravated NASH in C-kit deficient mice. For therapeutic purpose, C-kit+ LSECs were implanted into NASH or aged mice, which counteracted LSEC senescence and improved diet or aging-induced NASH by restoring the homeostasis of pericentral liver endothelium.
Comparison of Oxford Nanopore Technologies and Illumina MiSeq sequencing with mock communities and agricultural soil
Authors: Stevens Bo , Creed Timothy , Reardon Catherine , Manter Daniel 
Published: June 2022

Abstract
Illumina MiSeq is the current standard for characterizing microbial communities in soil. The Oxford Nanopore Technologies MinION sequencer is quickly gaining popularity because of the low initial cost and longer sequence reads. However, the accuracy of MinION, per base, is much lower than MiSeq (95% versus 99.9%). The effects of this difference in base-calling accuracy on taxonomic and diversity estimates remains unclear. We compared the output of short MiSeq to short and full-length MinION 16S rRNA amplicons from a mock community and agricultural soil samples. For all three methods, we found that taxonomic assignments of the mock community at both the genus and species level matched expectations with minimal deviation (genus: 80.9–90.5%; species: 70.9–85.2% Bray-Curtis similarity); however, the short MiSeq with error correction (DADA2) resulted in the correct estimate of species richness and much lower alpha diversity for soils. Several filtering strategies were tested to improve these estimates with varying results. The sequencing platform also had a significant influence on the relative abundances of taxa with MiSeq resulting in significantly higher abundances Actinobacteria, Chloroflexi, and Gemmatimonadetes and lower abundances of Acidobacteria, Bacteroides, Firmicutes, Proteobacteria, and Verrucomicrobia compared to the MinION platform. When comparing agricultural soils from two different sites (Fort Collins, CO and Pendleton, OR) methods varied in the taxa identified as significantly different between sites. At all taxonomic levels, the full-length MinION method had the highest similarity to the short MiSeq method with DADA2 correction with 73.2%, 69.3%, 74.1%, 79.3%, 79.4%, and 82.28% of the taxa at the phyla, class, order, family, genus, and species levels, respectively, showing similar patterns in differences between the sites. In summary, although both platforms appear suitable for 16S rRNA microbial community composition, biases for different taxa may make the comparison between studies problematic; and even with a single study (i.e., comparing sites or treatments), the sequencing platform can influence the differentially abundant taxa identified.
Coupled Discrete Phase Model and Eulerian Wall Film Model for Numerical Simulation of Respiratory Droplet Generation during Coughing
Authors: ANZAI Hitomi , SHINDO Yugo , KOHATA Yutaro , HASEGAWA Masahiro , TAKANA Hidemasa , MATSUNAGA Tetsuro , AKAIKE Takaaki , OHTA Makoto 
Published: June 2022

Abstract
Computational fluid dynamics is widely used to simulate droplet-spreading behavior due to respiratory events. However, droplet generation inside the body, such as the number, mass, and particle size distribution, has not been quantitatively analyzed. The aim of this study was to identify quantitative characteristics of droplet generation during coughing. Airflow simulations were performed by coupling the discrete phase model and Eulerian wall film model to reproduce shear-induced stripping of airway mucosa. An ideal airway model with symmetric bifurcations was constructed, and the wall domain was covered by a mucous liquid film. The results of the transient airflow simulation indicated that the droplets had a wide particle size distribution of 0.1–400 µm, and smaller droplets were generated in larger numbers. In addition, the total mass and number of droplets generated increased with an increasing airflow. The total mass of the droplets also increased with an increasing mucous viscosity, and the largest number and size of droplets were obtained at a viscosity of 8 mPa·s. The simulation methods used in this study can be used to quantify the particle size distribution and maximum particle diameter under various conditions.
ExAdEx: A simple method to maintain human adipose tissue in long-term cultures reveals differential expansion of adipose progenitor cell subpopulations in fibrotic and pro-inflammatory microenvironments.
Authors: DANI Vincent , BRUNI-FAVIER Solène , CHIGNON-SICARD Bérengère , LOUBAT Agnès , DOGLIO Alain , DANI Christian 
Published: June 2022

Abstract

A relevant model to explore how to promote the expansion of adipose progenitor cells (APCs) in lean and obese contexts is still lacking, as current adipocyte models have serious limitations. In this work, we describe a novel adipose tissue model, named ExAdEx, that can be obtained from cosmetic surgery wastes. ExAdEx products are adipose tissue units maintaining the characteristics and organization of adipose tissue as it presents in vivo. The model was viable and metabolically active for up to two months and could adopt a pathological-like phenotype. The results revealed that inflammatory and fibrotic microenvironments differentially regulated the expansion of the CD26 APC subpopulation and its CD54 and CD142 APC progenies. The approach used significantly improves the method of generating adipose tissue models, and ExAdEx constitutes a relevant model of human adipose tissue that could be used to identify pathways promoting expansion of APCs in physiological and pathological contexts.
Comprehensive Analysis of FRAS1/FREM family as Potential Biomarkers and Therapeutic Targets in Renal Clear Cell Carcinoma
Authors: Wang Ganggang , Wang Zheng , Lu Haiquan , Guo Liqiang , Kong Feng , Wang Aizhen , Zhao Shengtian 
Published: June 2022

Abstract
Background
FRAS1 (Fraser syndrome protein 1), together with FREM1 (the Fras1-related extracellular matrix proteins 1) and FREM2, belonging to the FRAS1/FREM extracellular matrix protein family, are considered to play essential roles in renal organogenesis and cancer progression. However, their roles in kidney renal clear cell carcinoma (KIRC) remain to be elucidated.
Methods
FRAS1/FREM RNA expression analysis was performed using TCGA/GTEx databases, and valided using GEO databases. Protein expression was peformed using CPTAC databases. Herein, we employed an array of bioinformatics methods and online databases to explore the potential oncogenic roles of FRAS1/FREM in KIRC.
Results
We found that FRAS1, FREM1 and FREM2 genes and proteins expression were significantly decreased in KIRC tissues than in normal tissues. Decreased FRAS1/FREM expression were significantly associated with advanced clinicopathological parameters (pathological stage, grade and tumor metastasis status). Notably, the patients with decreased FRAS1/FREM2 expression showed a high propensity for metastasis and poor prognosis. FRAS1/FREM were correlated with various immune infiltrating cells, especially CD4 + T cells and its corresponding subsets (Th1, Th2, Tfh and Tregs). FRAS1 and FREM2 had association with DNA methylation and their single CpG methylation levels were associated with prognosis. Moreover, FRAS1/FREM might exert antitumor effects by functioning in key oncogenic signalling pathways and metabolic pathways.
Conclusions
Together, our results indicated that FRAS1/FREM family members could be potential therapeutic targets and valuable prognostic biomarkers of KIRC.