bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanical loading of cranial joints minimizes the craniofacial phenotype in Crouzon syndrome
Authors: Moazen Mehran , Hejazi Mahbubeh , Savery Dawn , Jones Dominic , Marghoub Arsalan , Alazmani Ali , Pauws Erwin 
Published: June 2022

AbstractChildren with syndromic forms of craniosynostosis undergo a plethora of surgical interventions to resolve the clinical features caused by the premature fusion of cranial sutures. While surgical correction is reliable, the need for repeated rounds of invasive treatment puts a heavy burden on the child and their family. This study explores a non-surgical alternative using mechanical loading of the cranial joints to prevent or delay craniofacial phenotypes associated with Crouzon syndrome. We treated Crouzon syndrome mice before the onset of craniosynostosis by cyclical mechanical loading of cranial joints using a custom designed set-up. Cranial loading applied to the frontal bone partially restores normal skull morphology, significantly reducing the typical brachycephalic appearance. This is underpinned by the delayed closure of the coronal suture and of the intersphenoidal synchondrosis. This study provides a novel treatment alternative for syndromic craniosynostosis which has the potential to be an important step towards replacing, reducing or refining the surgical treatment of all craniosynostosis patients.
Controls on coastal flooding in the southern Baltic Sea revealed from the late Holocene sedimentary records
Authors: Leszczyńska Karolina , Stattegger Karl , Moskalewicz Damian , Jagodziński Robert , Kokociński Mikołaj , Niedzielski Przemysław , Szczuciński Witold 
Published: June 2022

AbstractClimate change and related sea-level rise pose significant threats to lowland coasts. However, the role of key controlling factors responsible for the frequency and landward extent of extreme storm surges is not yet fully understood. Here, we present a high-resolution sedimentary record of extreme storm surge flooding from the non-tidal southern Baltic Sea, spanning two periods: 3.6–2.9 ka BP and 0.7 ka BP until present. Sediments from coastal wetland, including sandy event layers, were analyzed by sedimentological (grain size, loss-on-ignition, micromorphology), geochronological (14C), geochemical (XRF), mineralogical (heavy minerals) and micropaleontological (diatoms) methods. The results show that both periods were characterized by high-frequency of storm surge flooding, in order of 1.3–4.2 events per century. These periods correlate with phases of enhanced storminess in northwest Europe and took place during both rising and fluctuating sea levels. The study shows that the frequency and landward extent of coastal inundation, largely depended on the development of natural barriers (e.g. beach ridges and aeolian foredunes). Thus, in the context of the future coastal storm-surge hazard, the protection of existing coastal barriers and their morphology is essential.
Rapid and sensitive magnetic field sensor based on photonic crystal fiber with magnetic fluid infiltrated nanoholes
Authors: Azad Saeed , Mishra Satyendra Kumar , Rezaei Ghasem , Izquierdo Ricardo , Ung Bora 
Published: June 2022

AbstractA fast response time (0.1 s) magnetic field sensor has been demonstrated utilizing a photonic crystal fiber with nano-size air holes infiltrated with polyethylene glycol based magnetic fluid. The effect of magnetic nanoparticles concentration in the fluid on the magneto-optical sensor performance and its dependence under varying magnetic-field loads was investigated in detail. In particular, the sensor response was analytically modelled with a Langevin function with a good fit (R$$\ge $$
≥
0.996). A threshold sensing point as low as 20 gauss was recorded and a detection range of 0–350 gauss was demonstrated by means of optical transmission measurements. The experimental results were validated by theory using a waveguide light transmission model fed by finite-element method simulations of the principal guided modes in the infiltrated fiber sensor. The simple interrogation scheme, high sensitivity and quick response time makes the proposed hybrid fiber-optic magneto-fluidic probe a promising platform for novel biochemical sensing applications.
Temperature models of development for Necrodes littoralis L. (Coleoptera: Silphidae), a carrion beetle of forensic importance in the Palearctic region
Authors: Gruszka Joanna , Matuszewski Szymon 
Published: June 2022

AbstractAnalysis of insects can provide evidence in death cases, for example, by answering the question about the time of death. Apart from flies, beetles are the second most useful insect group in forensic entomology. To elucidate the time of death based on insect evidence, developmental models of a given species are necessary. In this study, we developed such models for Necrodes littoralis, a necrophagous beetle, which is common in the Palearctic region and has great potential in forensic entomology. We monitored the development at 10 constant temperatures (14–30 °C). Larvae were reared in aggregations. Thermal summation models, isomorphen and isomegalen diagrams and growth curves were derived using the data. Depending on the temperature, development lasted between about 23 and 89 days. Mortality was high at the extremes of the temperature range. The thermal summation constant for the total development was 434.7 ± 28.86 accumulated degree-days above a developmental threshold of 9.04 ± 0.55 °C. This is the first comprehensive dataset on the development of N. littoralis. Implications for its use in forensic casework are discussed.
Rings of light caused by gravitational waves
Authors: Batic Davide , Faraji Joud Mojahed , Nowakowski Marek , Baracaldo Nicolas Maldonaldo 
Published: June 2022

AbstractWe reconsider the case of the geodesic motion of a massive and massless beam of test particles in a gravitational wave. In particular, we use a direct Lagrangian approach which simplifies the calculation. Our findings differ partly from previously performed calculations The final result can be interpreted as rings of light seen by the observer. We give a new interpretation to this picture and show that over large distances the effect, albeit rare, could in principle be observable.
ITRAQ-based quantitative proteomic analysis reveals that VPS35 promotes the expression of MCM2-7 genes in HeLa cells
Authors: Hong Xian , Wang Tao , Du Juan , Hong Yu , Yang Cai-Ping , Xiao Wei , Li Yang , Wang Ming , Sun He , Deng Zhi-Hui 
Published: June 2022

AbstractVacuolar protein sorting 35 (VPS35) is a major component of the retromer complex that regulates endosomal trafficking in eukaryotic cells. Recent studies have shown that VPS35 promotes tumor cell proliferation and affects the nuclear accumulation of its interacting partner. In this study, isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry were used to measure the changes in nuclear protein abundance in VPS35-depleted HeLa cells. A total of 47 differentially expressed proteins were identified, including 27 downregulated and 20 upregulated proteins. Gene ontology (GO) analysis showed that the downregulated proteins included several minichromosome maintenance (MCM) proteins described as cell proliferation markers, and these proteins were present in the MCM2-7 complex, which is essential for DNA replication. Moreover, we validated that loss of VPS35 reduced the mRNA and protein expression of MCM2-7 genes. Notably, re-expression of VPS35 in VPS35 knockout HeLa cells rescued the expression of these genes. Functionally, we showed that VPS35 contributes to cell proliferation and maintenance of genomic stability of HeLa cells. Therefore, these findings reveal that VPS35 is involved in the regulation of MCM2-7 gene expression and establish a link between VPS35 and cell proliferation.
Leveraging electronic medical record functionality to capture adenoma detection rate
Authors: Jones Blake , Scott Frank I. , Espinoza Jeannine , Laborde Sydney , Chambers Micah , Wani Sachin , Edmundowicz Steven , Austin Gregory , Pell Jonathan , Patel Swati G. 
Published: June 2022

AbstractMeasuring the adenoma detection rate (ADR) is critical to providing quality care, however it is also challenging. We aimed to develop a tool using pre-existing electronic health record (EHR) functions to accurately and easily measure total ADR and to provide real-time feedback for endoscopists. We utilized the Epic EHR. With the help of an Epic analyst, using existing tools, we developed a method by which endoscopy staff could mark whether an adenoma was detected for a given colonoscopy. Using these responses and all colonoscopies performed by the endoscopist recorded in the EHR, ADR was calculated in a report and displayed to endoscopists within the EHR. One endoscopist piloted the tool, and results of the tool were validated against a manual chart review. Over the pilot period the endoscopist performed 145 colonoscopies, of which 78 had adenomas. The tool correctly identified 76/78 colonoscopies with an adenoma and 67/67 of colonoscopies with no adenomas (97.4% sensitivity, 100% specificity, 98% accuracy). There was no difference in ADR as determined by the tool compared to manual review (53.1% vs. 53.8%, p = 0.912). We successfully developed and pilot tested a tool to measure ADR using existing EHR functionality.
Repeatable detection of Ag+ ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with microfluidic heating system
Authors: Yoshida Koki , Hayashi Tomoki , Takinoue Masahiro , Onoe Hiroaki 
Published: June 2022

AbstractThis paper describes repeatable detection of Ag+ ions using a DNA aptamer-linked hydrogel biochemical sensor integrated with a microfluidic heating system. Biochemical sensors that respond to chemical compounds and produce detectable signals have a critical role in many aspects of modern society. In particular, the repeatable measurement of environmental information such as toxic substances including Ag+ ions could be expected to improve the environment. The DNA aptamer is an attractive candidate because of the stability and the selectivity of binding to chemicals. However, previous DNA aptamer biochemical sensors could not measure repeatedly because those sensors did not have initializing functions. To overcome this challenge, we proposed a DNA aptamer-linked hydrogel biochemical sensor integrated with the microfluidic heating system enabling repeatable detection of Ag+ ions. The binding Ag+ ions are dissociated by heating and flushing through the integrated microfluidic heating device. The DNA aptamer-linked hydrogel had the capability to detect a wide range of Ag+ ion concentrations (10−5–10 mM) including a toxic range for various aquatic organisms. Finally, we demonstrated the repeatable detection of the Ag+ ions. These results indicated that our proposed biochemical sensor is expected to use for long-term monitoring with high stability in ambient temperature and low power consumption.
Constitutive androstane receptor and pregnane X receptor genotype influence efavirenz plasma concentration and CYP2B6 enzyme activity
Authors: Petros Zelalem , Habtewold Abiy , Makonnen Eyasu , Aklillu Eleni 
Published: June 2022

AbstractEfavirenz is metabolized by CYP2B6, an inducible enzyme whose expression is regulated by the constitutive androstane receptor and pregnane X receptor nuclear receptors. CAR and PXR are encoded by genetically polymorphic NR1I2 and NR1I3, respectively. We examined the impact of NR1I2 and NR1I3 genotype on plasma EFV concentration and CYP2B6 enzyme activity among TB-HIV co-infected patients in Ethiopia. Treatment-naïve HIV patients with TB co-infection (n = 80) were enrolled and received first-line EFV-based antiretroviral and rifampicin-based anti-TB therapy. Plasma EFV and 8-hydroxy-EFV concentrations at the 4th and 16th week of EFV treatment were determined using LC/MS/MS. EFV/8-hydroxy-EFVmetabolic ratio was used as CYP2B6 metabolic activity index. In multivariate regression analysis, NR1I3 rs3003596C or NR1I2 rs2472677T variant allele carriers had significantly lower plasma EFV concentrations than non-carriers. Patients with NR1I2 rs3814057C/C genotype or NR1I3 rs3003596C allele carriers had significantly lower mean log EFV MR. Among CYP2B6*6 allele carriers, patients with NR1I3 rs2502815T/T or NR1I2 rs3814057C/C genotype had significantly lower mean log EFV MR. In conclusion, genetic variants in NR1I2 and NR1I3 genes influence plasma EFV exposure and CYP2B6 enzyme activity in TB-HIV co-infected patients on drug treatment.
Antibody–nanobody combination increases their neutralizing activity against SARS-CoV-2 and nanobody H11-H4 is effective against Alpha, Kappa and Delta variants
Authors: Nguyen Hung , Li Mai Suan 
Published: June 2022

AbstractThe global spread of COVID-19 is devastating health systems and economies worldwide. While the use of vaccines has yielded encouraging results, the emergence of new variants of SARS-CoV-2 shows that combating COVID-19 remains a big challenge. One of the most promising treatments is the use of not only antibodies, but also nanobodies. Recent experimental studies revealed that the combination of antibody and nanobody can significantly improve their neutralizing ability through binding to the SARS-CoV-2 spike protein, but the molecular mechanisms underlying this observation remain largely unknown. In this work, we investigated the binding affinity of the CR3022 antibody and H11-H4 nanobody to the SARS-CoV-2 receptor binding domain (RBD) using molecular modeling. Both all-atom steered molecular dynamics simulations and coarse-grained umbrella sampling showed that, consistent with the experiment, CR3022 associates with RBD more strongly than H11-H4. We predict that the combination of CR3022 and H11-H4 considerably increases their binding affinity to the spike protein. The electrostatic interaction was found to control the association strength of CR3022, but the van der Waals interaction dominates in the case of H11-H4. However, our study for a larger set of nanobodies and antibodies showed that the relative role of these interactions depends on the specific complex. Importantly, we showed Beta, Gamma, Lambda, and Mu variants reduce the H11-H4 activity while Alpha, Kappa and Delta variants increase its neutralizing ability, which is in line with experiment reporting that the nanobody elicited from the llama is very promising for fighting against the Delta variant.
Multiplexed spatially-focused localization of light in adipose biological tissues
Authors: Bykov Alexander , Tuchin Valery , Meglinski Igor 
Published: June 2022

AbstractLast decades the effects of localization and focusing of light in turbid randomly inhomogeneous tissue-like scattering medium have been attracting a particular attention. Weak localization of light in disordered and weakly ordered biological tissue, polarization memory effect, correlations in transmission matrices, focusing light by wavefronts shaping have been widely exploited. Here, we represent an experimentally observed and theoretically confirmed new type of spatial localization of light within biological tissues. General description of the observed phenomenon based on Monte Carlo ray tracing model is provided. We find that innate body arrangements of individual adipocytes can act as a cascade of quasi-ordered microscale lenses confining propagation of light within adipose tissues similar to lens lightguides. The observed spatially-resolved longitudinal multi-focusing of light within disordered adipose biological tissues can naturally lead greater spatial control and enhance light-tissue interactions.
Sparse regularization method combining SVA for feature enhancement of SAR images
Authors: Xu Zhongqiu , Zhou Guoru , Zhang Bingchen , Zhang Zhe , Wu Yirong 
Published: June 2022

AbstractSparse signal processing has been widely used in synthetic aperture radar imaging and feature enhancement of images in the recent decade. Sparse regularization ℓ1 can reduce the imaging noise level and suppress sidelobes. However, the suppression of sidelobes by sparse regularization often pays the price of losing information of weak targets. Therefore, the sparse regularization method combining spatially variant apodization is proposed in this paper, which can suppress noise, sidelobes and retain detail information. The performance of the proposed method is verified using simulated and real data.
Real‐time traffic cone detection for autonomous driving based on YOLOv4
Authors: Su Qinghua , Wang Haodong , Xie Min , Song Yue , Ma Shaobo , Li Boxiong , Yang Ying , Wang Liyong 
Published: June 2022

AbstractA temporary road composed of traffic cones is an indispensable practical scene for the realization of automatic driving technology. However, the detection of traffic cones is a challenging issue because of their small volume and unfixed position. This work proposes a novel method that fuses colour and depth image information for traffic cone detection. Traffic cones are captured by a special machine vision system consisting of two monochrome cameras for cone distance perception and two colour cameras for cone colour acquisition. Via the YOLOv4 algorithm based on the Darknet platform and a detection result matching algorithm, the position of the traffic cone can be obtained and path planning can be performed. The results of experiments show that the proposed method can recognize red, blue, and yellow traffic cones in colour images with an average detection time of 35.46 ms and respective accuracies of 97.51%, 98.63%, and 97.29%. Compared with the previous traffic cone detection research, the proposed algorithm was found to exhibit advantages in small target sensitivity and overall detection accuracy in both static and dynamic experiments.
A multi‐antenna super‐resolution passive Wi‐Fi radar algorithm: Combined model order selection and parameter estimation
Authors: Yildirim Hasan Can , Storrer Laurent , De Doncker Philippe , Louveaux Jerôme , Horlin François 
Published: June 2022

AbstractIn recent years, Wi‐Fi has become the main gateway that connects users to the Internet. Considering the availability of Wi‐Fi signals, and their suitability for channel estimation, IEEE established the Wi‐Fi Sensing (WS) Task Group whose purpose is to study the feasibility of Wi‐Fi‐based environment sensing. However, Wi‐Fi signals are transmitted over limited bandwidths with a relatively small number of antennas in bursts, fundamentally limiting the range, Angle‐of‐Arrival and speed resolutions. This paper presents a super‐resolution algorithm to perform the parameter estimation in a quasi‐monostatic WS scenario. The proposed algorithm, RIVES, estimates the range, Angle‐of‐Arrival and speed parameters with Vandermonde decomposition of Hankel matrices. To estimate the size of the signal subspace, RIVES uses a novel Model Order Selection method which eliminates spurious noise targets based on their distance to the noise and signal subspaces. Various scenarios with multiple targets are simulated to show the robustness of RIVES. In order to prove its accuracy, real‐life indoor experiments are conducted with human targets by using Software Defined Radios.
Correlation of Ultrasound-Based Hydration Assessment Measures with CVP and Clinical Hydration Status among Children Admitted to the PICU: A Prospective Observational Study
Authors: Tripathi Sandeep , Osman Tara , Hafzalah Mina , Lee Kejin , Whalen Drew A. 
Published: June 2022

Abstract
Purpose This article assesses the correlation of respiratory variation in inferior vena cava (IVC) with central venous pressure (CVP) in children. Secondary objective was to evaluate IVC variability with clinical hydration status.
Methods IVC variability was assessed at the subcostal (SC) and right lateral (RL) region, and collapsibility index (CI) (spontaneously breathing) and distensibility index (DI) (positive pressure) and IVC/aortic ratio were calculated. Partial correlations were calculated between CI/DI with CVP adjusting for body mass index and age. Sensitivity of CI and DI to predict clinical dehydration was calculated using receiver operating characteristic curves.
Results A total of 145 ultrasounds were performed on 72 patients (41% positive pressure). Only RL CI in spontaneously breathing patients strongly correlated with CVP (r = –0.65, p < 0.001). A moderate correlation was observed between CI and DI from SC and RL regions (r's = 0.38 and 0.47). Among spontaneously breathing patients, a significant difference was observed in the SC CI based on hydration status. For patients on positive pressure, IVC/aortic ratio had a significant difference. SC CI had the highest area under the curve (0.82) to detect dehydration with 80% sensitivity/87% specificity for a cutoff of 40%.
Conclusion SC CI is the most reliable measure to assess the hydration status of spontaneously breathing children, while the IVC/aortic ratio performs well for patients under positive pressure. RL CI has strong negative correlation with CVP in spontaneously breathing patients.
Adaptation of a Public–Private Partnership Model for the Implementation of Minipool Nucleic Acid Testing for Screening Routine Blood Donations and Assay Evaluation
Authors: Mahapatra Smita , Parida Pankaj , Prasad Chitta Ranjan , Palai Sabita , Mishra Debashis , Behera Susmita , Mishra Rabindra Kumar , Prusty Binayak Prasad , Sarkar Kalyan 
Published: June 2022

Abstract
Background Nucleic acid amplification testing (NAT) for the screening of blood donations is known to improve blood safety. The decision to initiate NAT requires careful deliberation of infrastructure, skilled manpower, and financial resources. This report outlines the initiative of the Government of Odisha to implement NAT screening in government blood banks in the state of Odisha, India, through public–private partnership (PPP) and evaluates the incremental yield of minipool NAT screening over serology testing of blood donations.
Methods Blood donations collected between June 2016 and September 2018 were initially screened for HBV (HBsAg), HCV (anti-HCV), and HIV (anti-HIV-1 and HIV-2) by ELISA, and syphilis and malaria. Sero-nonreactive donations were further screened in pools of six by Roche cobas TaqScreen MPX test version 2.0 (MPX2) NAT.
Results On screening 3,39,472 blood donations, 1.34% seroreactive donations were detected. In all, 847 NAT-reactive donations (0.26%): 693 HBV, 58 HCV, and 96 HIV were detected. The NAT yields were 1:386 overall, 1:472 for HBV, 1:5642 for HCV, and 1:3409 for HIV.
Conclusion NAT testing using the highly sensitive MPX2 assay leads to incremental detection of TTIs over serology. Implementation of NAT along with serological testing in blood centers all over India will be an important step towards providing safe blood. Our study not only highlights the benefits of minipool NAT testing but also presents a scalable PPP model that can serve as a template for application across other states.
Immunity in Stroke: The Next Frontier
Authors: Li Ting , Liesz Arthur 
Published: June 2022

AbstractTranslational stroke research has long been focusing on neuroprotective strategies to prevent secondary tissue injury and promote recovery after acute ischemic brain injury. The inflammatory response to stroke has more recently emerged as a key pathophysiological pathway contributing to stroke outcome. It is now accepted that the inflammatory response is functionally involved in all phases of the ischemic stroke pathophysiology. The immune response is therefore considered a breakthrough target for ischemic stroke treatment. On one side, stroke induces a local neuroinflammatory response, in which the inflammatory activation of glial, endothelial and brain-invading cells contributes to lesion progression after stroke. On the other side, ischemic brain injury perturbs systemic immune homeostasis and results in long-lasting changes of systemic immunity. Here, we briefly summarize current concepts in local neuroinflammation and the systemic immune responses after stroke, and highlight two promising therapeutic strategies for poststroke inflammation.