bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two-dimensional covalent organic framework films prepared on various substrates through vapor induced conversion
Authors: Liu Minghui , Liu Youxing , Dong Jichen , Bai Yichao , Gao Wenqiang , Shang Shengcong , Wang Xinyu , Kuang Junhua , Du Changsheng , Zou Ye , Chen Jianyi , Liu Yunqi 
Published: March 2022

AbstractCovalent organic frameworks (COFs) can exhibit high specific surface area and catalytic activity, but traditional solution-based synthesis methods often lead to insoluble and infusible powders or fragile films on solution surface. Herein we report large-area –C=N– linked two-dimensional (2D) COF films with controllable thicknesses via vapor induced conversion in a chemical vapor deposition (CVD) system. The assembly process is achieved by reversible Schiff base polycondensation between PyTTA film and TPA vapor, which results in a uniform organic framework film directly on growth substrate, and is driven by π‐π stacking interactions with the aid of water and acetic acid. Wafer-scale 2D COF films with different structures have been successfully synthesized by adjusting their building blocks, suggesting its generic applicability. The carrier mobility of PyTTA-TPA COF films can reach 1.89 × 10−3 cm2 V−1 s−1. When employed as catalysts in hydrogen evolution reaction (HER), they show high electrocatalytic activity compared with metal-free COFs or even some metallic catalysts. Our results represent a versatile route for the direct construction of large-area uniform 2D COF films on substrates towards multi-functional applications of 2D π‐conjugated systems.
Large evanescently-induced Brillouin scattering at the surrounding of a nanofibre
Authors: Yang Fan , Gyger Flavien , Godet Adrien , Chrétien Jacques , Zhang Li , Pang Meng , Beugnot Jean-Charles , Thévenaz Luc 
Published: March 2022

AbstractBrillouin scattering has been widely exploited for advanced photonics functionalities such as microwave photonics, signal processing, sensing, lasing, and more recently in micro- and nano-photonic waveguides. Most of the works have focused on the opto-acoustic interaction driven from the core region of micro- and nano-waveguides. Here we observe, for the first time, an efficient Brillouin scattering generated by an evanescent field nearby a single-pass sub-wavelength waveguide embedded in a pressurised gas cell, with a maximum gain coefficient of 18.90 ± 0.17 m−1W−1. This gain is 11 times larger than the highest Brillouin gain obtained in a hollow-core fibre and 79 times larger than in a standard single-mode fibre. The realisation of strong free-space Brillouin scattering from a waveguide benefits from the flexibility of confined light while providing a direct access to the opto-acoustic interaction, as required in free-space optoacoustics such as Brillouin spectroscopy and microscopy. Therefore, our work creates an important bridge between Brillouin scattering in waveguides, Brillouin spectroscopy and microscopy, and opens new avenues in light-sound interactions, optomechanics, sensing, lasing and imaging.
Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments
Authors: Kreutzberger Mark A. B. , Sobe Richard C. , Sauder Amber B. , Chatterjee Sharanya , Peña Alejandro , Wang Fengbin , Giron Jorge A. , Kiessling Volker , Costa Tiago R. D. , Conticello Vincent P. , Frankel Gad , Kendall Melissa M. , Scharf Birgit E. , Egelman Edward H. 
Published: March 2022

AbstractFlagellar filaments function as the propellers of the bacterial flagellum and their supercoiling is key to motility. The outer domains on the surface of the filament are non-critical for motility in many bacteria and their structures and functions are not conserved. Here, we show the atomic cryo-electron microscopy structures for flagellar filaments from enterohemorrhagic Escherichia coli O157:H7, enteropathogenic E. coli O127:H6, Achromobacter, and Sinorhizobium meliloti, where the outer domains dimerize or tetramerize to form either a sheath or a screw-like surface. These dimers are formed by 180° rotations of half of the outer domains. The outer domain sheath (ODS) plays a role in bacterial motility by stabilizing an intermediate waveform and prolonging the tumbling of E. coli cells. Bacteria with these ODS and screw-like flagellar filaments are commonly found in soil and human intestinal environments of relatively high viscosity suggesting a role for the dimerization in these environments.
FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity
Authors: Cui Zifeng , Tian Rui , Huang Zhaoyue , Jin Zhuang , Li Lifang , Liu Jiashuo , Huang Zheying , Xie Hongxian , Liu Dan , Mo Haiyan , Zhou Rong , Lang Bin , Meng Bo , Weng Haiyan , Hu Zheng 
Published: March 2022

AbstractGenome editing technologies hold tremendous potential in biomedical research and drug development. Therefore, it is imperative to discover gene editing tools with superior cutting efficiency, good fidelity, and fewer genomic restrictions. Here, we report a CRISPR/Cas9 from Faecalibaculum rodentium, which is characterized by a simple PAM (5′-NNTA-3′) and a guide RNA length of 21–22 bp. We find that FrCas9 could achieve comparable efficiency and specificity to SpCas9. Interestingly, the PAM of FrCas9 presents a palindromic sequence, which greatly expands its targeting scope. Due to the PAM sequence, FrCas9 possesses double editing-windows for base editor and could directly target the TATA-box in eukaryotic promoters for TATA-box related diseases. Together, our results broaden the understanding of CRISPR/Cas-mediated genome engineering and establish FrCas9 as a safe and efficient platform for wide applications in research, biotechnology and therapeutics.
In situ infrared nanospectroscopy of the local processes at the Li/polymer electrolyte interface
Authors: He Xin , Larson Jonathan M. , Bechtel Hans A. , Kostecki Robert 
Published: March 2022

AbstractSolid-state batteries possess the potential to significantly impact energy storage industries by enabling diverse benefits, such as increased safety and energy density. However, challenges persist with physicochemical properties and processes at electrode/electrolyte interfaces. Thus, there is great need to characterize such interfaces in situ, and unveil scientific understanding that catalyzes engineering solutions. To address this, we conduct multiscale in situ microscopies (optical, atomic force, and infrared near-field) and Fourier transform infrared spectroscopies (near-field nanospectroscopy and attenuated total reflection) of intact and electrochemically operational graphene/solid polymer electrolyte interfaces. We find nanoscale structural and chemical heterogeneities intrinsic to the solid polymer electrolyte initiate a cascade of additional interfacial nanoscale heterogeneities during Li plating and stripping; including Li-ion conductivity, electrolyte decomposition, and interphase formation. Moreover, our methodology to nondestructively characterize buried interfaces and interphases in their native environment with nanoscale resolution is readily adaptable to a number of other electrochemical systems and battery chemistries.
De novo emergence of a remdesivir resistance mutation during treatment of persistent SARS-CoV-2 infection in an immunocompromised patient: a case report
Authors: Gandhi Shiv , Klein Jonathan , Robertson Alexander J. , Peña-Hernández Mario A. , Lin Michelle J. , Roychoudhury Pavitra , Lu Peiwen , Fournier John , Ferguson David , Mohamed Bakhash Shah A. K. , Catherine Muenker M. , Srivathsan Ariktha , Wunder Elsio A. , Kerantzas Nicholas , Wang Wenshuai , Lindenbach Brett , Pyle Anna , Wilen Craig B. , Ogbuagu Onyema , Greninger Alexander L. , Iwasaki Akiko , Schulz Wade L. , Ko Albert I. 
Published: March 2022

AbstractSARS-CoV-2 remdesivir resistance mutations have been generated in vitro but have not been reported in patients receiving treatment with the antiviral agent. We present a case of an immunocompromised patient with acquired B-cell deficiency who developed an indolent, protracted course of SARS-CoV-2 infection. Remdesivir therapy alleviated symptoms and produced a transient virologic response, but her course was complicated by recrudescence of high-grade viral shedding. Whole genome sequencing identified a mutation, E802D, in the nsp12 RNA-dependent RNA polymerase, which was not present in pre-treatment specimens. In vitro experiments demonstrated that the mutation conferred a ~6-fold increase in remdesivir IC50 but resulted in a fitness cost in the absence of remdesivir. Sustained clinical and virologic response was achieved after treatment with casirivimab-imdevimab. Although the fitness cost observed in vitro may limit the risk posed by E802D, this case illustrates the importance of monitoring for remdesivir resistance and the potential benefit of combinatorial therapies in immunocompromised patients with SARS-CoV-2 infection.
Control of zeolite framework flexibility for ultra-selective carbon dioxide separation
Authors: Du Peng , Zhang Yuting , Wang Xuerui , Canossa Stefano , Hong Zhou , Nénert Gwilherm , Jin Wanqin , Gu Xuehong 
Published: March 2022

AbstractMolecular sieving membranes with uniform pore size are highly desired for carbon dioxide separation. All-silica zeolite membranes feature well-defined micropores, but the size-exclusion effect is significantly compromised by the non-selective macro-pores generated during detemplation. Here we propose a template modulated crystal transition (TMCT) approach to tune the flexibility of Decadodecasil 3 R (DD3R) zeolite to prepare ultra-selective membranes for CO2/CH4 separation. An instantaneous overheating is applied to synchronize the template decomposition with the structure relaxation. The organic template molecules are transitionally converted to tight carbon species by the one-minute overheating at 700 °C, which are facilely burnt out by a following moderate thermal treatment. The resulting membranes exhibit CO2/CH4 selectivity of 157~1,172 and CO2 permeance of (890~1,540) × 10−10 mol m−2 s−1 Pa−1. The CO2 flux and CO2/CH4 mixture selectivity reach 3.6 Nm3 m−2 h−1 and 43 even at feed pressure up to 31 bar. Such strategy could pave the way of all-silica zeolite membranes to practical applications.
Structure and function of the soil microbiome underlying N2O emissions from global wetlands
Authors: Bahram Mohammad , Espenberg Mikk , Pärn Jaan , Lehtovirta-Morley Laura , Anslan Sten , Kasak Kuno , Kõljalg Urmas , Liira Jaan , Maddison Martin , Moora Mari , Niinemets Ülo , Öpik Maarja , Pärtel Meelis , Soosaar Kaido , Zobel Martin , Hildebrand Falk , Tedersoo Leho , Mander Ülo 
Published: March 2022

AbstractWetland soils are the greatest source of nitrous oxide (N2O), a critical greenhouse gas and ozone depleter released by microbes. Yet, microbial players and processes underlying the N2O emissions from wetland soils are poorly understood. Using in situ N2O measurements and by determining the structure and potential functional of microbial communities in 645 wetland soil samples globally, we examined the potential role of archaea, bacteria, and fungi in nitrogen (N) cycling and N2O emissions. We show that N2O emissions are higher in drained and warm wetland soils, and are correlated with functional diversity of microbes. We further provide evidence that despite their much lower abundance compared to bacteria, nitrifying archaeal abundance is a key factor explaining N2O emissions from wetland soils globally. Our data suggest that ongoing global warming and intensifying environmental change may boost archaeal nitrifiers, collectively transforming wetland soils to a greater source of N2O.
Binary-state scanning probe microscopy for parallel imaging
Authors: Kim Gwangmook , Kim Eoh Jin , Do Hyung Wan , Cho Min-Kyun , Kim Sungsoon , Kang Shinill , Kim Dohun , Cheon Jinwoo , Shim Wooyoung 
Published: March 2022

AbstractScanning probe microscopy techniques, such as atomic force microscopy and scanning tunnelling microscopy, are harnessed to image nanoscale structures with an exquisite resolution, which has been of significant value in a variety of areas of nanotechnology. These scanning probe techniques, however, are not generally suitable for high-throughput imaging, which has, from the outset, been a primary challenge. Traditional approaches to increasing the scalability have involved developing multiple probes for imaging, but complex probe design and electronics are required to carry out the detection method. Here, we report a probe-based imaging method that utilizes scalable cantilever-free elastomeric probe design and hierarchical measurement architecture, which readily reconstructs high-resolution and high-throughput topography images. In a single scan, we demonstrate imaging with a 100-tip array to obtain 100 images over a 1-mm2 area with 106 pixels in less than 10 min. The potential for large-scale tip integration and the advantage of a simple probe array suggest substantial promise for our approach to high-throughput imaging far beyond what is currently possible.
Vital signs in pediatric oncology patients assessed by continuous recording with a wearable device, NCT04134429
Authors: Haemmerli Marion , Ammann Roland A. , Roessler Jochen , Koenig Christa , Brack Eva 
Published: March 2022

AbstractPediatric patients with cancer are at high risk for severe infections. Changes in vital signs, triggered by infections, may be detected earlier by continuous recording with a wearable device than with discrete measurements. This prospective, observational single-center feasibility study consecutively recruited pediatric patients undergoing chemotherapy for cancer. The WD Everion® was used for 14 days in each of the 20 patients on study to continuously record vital signs. Nine different vital signs and health indicators derived from them, plus six quality scores. This resulted in 274 study days (6576 hours) with 85’854 measuring points, which are a total of 772’686 measurements of vital signs and health indicators, plus 515’124 quality scores. Additionally, non-WD data like side effects, acceptability of the WD and effort for investigators were collected. In this manuscript, we present the methods of acquisition and explanations to the complete data set, which have been made publically available on open access and which can be used to study feasibility of continuous multi-parameter recording of vital signs by a WD.
Trans-polar drift-pathways of riverine European microplastic
Authors: Huserbråten Mats B. O. , Hattermann Tore , Broms Cecilie , Albretsen Jon 
Published: March 2022

AbstractHigh concentrations of microplastic particles are reported across the Arctic Ocean–yet no meaningful point sources, suspension timelines, or accumulation areas have been identified. Here we use Lagrangian particle advection simulations to model the transport of buoyant microplastic from northern European rivers to the high Arctic, and compare model results to the flux of sampled synthetic particles across the main entrance to the Arctic Ocean. We report widespread dispersal along the Eurasian continental shelf, across the North Pole, and back into the Nordic Seas; with accumulation zones over the Nansen basin, the Laptev Sea, and the ocean gyres of the Nordic Seas. The equal distribution of sampled synthetic particles across water masses covering a wide time frame of anthropogenic influence suggests a system in full saturation rather than pronounced injection from European sources, through a complex circulation scheme connecting the entire Arctic Mediterranean. This circulation of microplastic through Arctic ecosystems may have large consequences to natural ecosystem health, highlighting an ever-increasing need for better waste management.
Filtered-orthogonal wavelet division multiplexing (F-OWDM) technique for 5G and beyond communication systems
Authors: Almutairi Ali. F. , Krishna A. 
Published: March 2022

AbstractFiltered-orthogonal frequency division multiplexing (F-OFDM) is one of the most protruding multicarrier modulation (MCM) techniques for fifth-generation and beyond wireless communication. However, it possesses a high peak-to-average power ratio (PAPR), which results in its poor performance. Thus, a novel wavelet based MCM technique, namely filtered orthogonal wavelet division multiplexing (F-OWDM), is proposed as an efficient alternative to conventional F-OFDM (C-F-OFDM) to reduce the PAPR. In this model, the traditional Fourier transforms (FT) as used in C-F-OFDM was replaced with Wavelet Transforms. The proposed F-OWDM system does not require a cyclic prefix because of the overlapping sub-carriers in the time and frequency domains. Thus, the F-OWDM system exhibits higher bandwidth efficiency. In this paper, the performance of the F-OWDM system with various wavelets from different wavelet families under an additive white Gaussian noise and flat fading channel is investigated. The PAPR and bit error rate (BER) of the F-OWDM system using Haar, discrete Meyer, bi-orthogonal, symlet, and Daubechies wavelets are analyzed. A comparative study between F-OWDM and C-F-OFDM is also presented. From the results, it is able to prove that F-OWDM has the advantage of lower PAPR and lower bit error rate than the C-F-OFDM system.
COVID-19 mortality in Italy varies by patient age, sex and pandemic wave
Authors: Minnai Francesca , De Bellis Gianluca , Dragani Tommaso A. , Colombo Francesca 
Published: March 2022

AbstractSARS-CoV-2 has caused a worldwide epidemic of enormous proportions, which resulted in different mortality rates in different countries for unknown reasons. We analyzed factors associated with mortality using data from the Italian national database of more than 4 million SARS-CoV-2-positive cases diagnosed between January 2020 and July 2021, including > 415 thousand hospitalized for coronavirus disease-19 (COVID-19) and > 127 thousand deceased. For patients for whom age, sex and date of infection detection were available, we determined the impact of these variables on mortality 30 days after the date of diagnosis or hospitalization. Multivariable weighted Cox analysis showed that each of the analyzed variables independently affected COVID-19 mortality. Specifically, in the overall series, age was the main risk factor for mortality, with HR > 100 in the age groups older than 65 years compared with a reference group of 15–44 years. Male sex presented a two-fold higher risk of death than female sex. Patients infected after the first pandemic wave (i.e. after 30 June 2020) had an approximately threefold lower risk of death than those infected during the first wave. Thus, in a series of all confirmed SARS-CoV-2-infected cases in an entire European nation, elderly age was by far the most significant risk factor for COVID-19 mortality, confirming that protecting the elderly should be a priority in pandemic management. Male sex and being infected during the first wave were additional risk factors associated with COVID-19 mortality.
Deep learning-based methods for natural hazard named entity recognition
Authors: Sun Junlin , Liu Yanrong , Cui Jing , He Handong 
Published: March 2022

AbstractNatural hazard named entity recognition is a technique used to recognize natural hazard entities from a large number of texts. The method of natural hazard named entity recognition can facilitate acquisition of natural hazards information and provide reference for natural hazard mitigation. The method of named entity recognition has many challenges, such as fast change, multiple types and various forms of named entities. This can introduce difficulties in research of natural hazard named entity recognition. To address the above problem, this paper constructed a natural disaster annotated corpus for training and evaluation model, and selected and compared several deep learning methods based on word vector features. A deep learning method for natural hazard named entity recognition can automatically mine text features and reduce the dependence on manual rules. This paper compares and analyzes the deep learning models from three aspects: pretraining, feature extraction and decoding. A natural hazard named entity recognition method based on deep learning is proposed, namely XLNet-BiLSTM-CRF model. Finally, the research hotspots of natural hazards papers in the past 10 years were obtained through this model. After training, the precision of the XLNet-BilSTM-CRF model is 92.80%, the recall rate is 91.74%, and the F1-score is 92.27%. The results show that this method, which is superior to other methods, can effectively recognize natural hazard named entities.
Irregular rupture propagation and geometric fault complexities during the 2010 Mw 7.2 El Mayor-Cucapah earthquake
Authors: Yamashita Shinji , Yagi Yuji , Okuwaki Ryo 
Published: March 2022

AbstractThe 2010 MW 7.2 El Mayor-Cucapah, Mexico, earthquake ruptured multiple faults with different faulting mechanisms. Resolving the earthquake rupture process and its relation to the geometric fault complexities is critical to our understanding of the earthquake source physics, but doing so by conventional finite-fault inversion is challenging because modelling errors due to inappropriate assumptions about the fault geometry distort the solution and make robust interpretation difficult. Here, using a potency density tensor approach to finite-fault inversion, we inverted the observed teleseismic P waveforms of the 2010 El Mayor-Cucapah earthquake to simultaneously estimate the rupture process and the fault geometry. We found that the earthquake consisted of an initial normal faulting rupture, which was followed by a strike-slip bilateral rupture towards the southeast and northwest that originated on the northwest side of the epicentre. The southeastern rupture propagated back through the initial rupture area, but with strike-slip faulting. Although the northwestern rupture propagated across the left step in the Puerta fault-accommodation zone, the rupture was temporarily stalled by the associated change of the fault geometry. These results highlight the irregular rupture process, which involved a back-propagating rupture and fluctuating rupture propagation controlled the complexity of the fault system.
Penetration ability and microhardness of infiltrant resin and two pit and fissure sealants in primary teeth with early enamel lesions
Authors: Memarpour Mahtab , Abedinzade Arefe , Rafiee Azade , Hashemian Atieh 
Published: March 2022

AbstractTo determine the penetration depth and enamel microhardness (EMH) of an infiltrant resin and two fissure sealants in primary teeth with early enamel lesions. We randomly divided 174 sound teeth into six groups (n = 29): (1) phosphoric acid (PA) + Clinpro, (2) PA + Aegis, (3) Icon, (4) hydrochloric acid (HCl) + Clinpro, (5) HCl + Aegis, and (6) control. Percentage penetration (%PP) was analyzed by confocal laser scanning microscopy (n = 15). EMH was measured (n = 12), and the percentage of EMH recovery (%REMH) was calculated. Twelve samples were examined under a scanning electron microscope (SEM). All data were analyzed with the Kruskal–Wallis and one-way ANOVA tests (p < 0.05). Groups 3 and 4 showed the highest %PP (all, p < 0.05). Icon application led to significantly higher %REMH compared to the others (p < 0.05). Groups 2 and 5 showed the lowest reduction in %REMH after pH-cycling. Application of Icon and Clinpro with HCl pretreatment showed the greatest %PP. pH-cycling led to a decrease in %REMH for all of the materials, although this effect was lower in teeth treated with Aegis.