bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous Determination of Adulterants in Dietary Food Supplements Using Multivariate Data Analysis after Preconcentration with Novel Nanosorbents and Chromatographic Measurement
Authors: Hosseini Ensie , Ghasemi Jahan B , Shekarchi Maryam 
Published: May 2022

Abstract

Background
The increasing popularity of dietary supplements and, consequently, related adulteration emphasizes the rising need to examine the association of food supplements with fraud. Intentional or unintentional fraud in food supplements by hazardous chemicals compounds is a problem that many countries are struggling with. Much effort have been made to effectively and reliably control the quality of food supplements.

Objective
Due to the importance of the subject, an analytical method for the simultaneous and reliable detection and quantitative determination of three key adulterants in dietary food supplements was developed. The proposed method benefits from analytical methods and multivariate calibration methods to progress the determination of adulterants in a complex matrix.

Methods
HPLC assisted by multivariate curve resolution-alternating least square (MCR-ALS) analysis was used to detect adulterants in real samples after separation and preconcentration using novel mesoporous carbon nanoparticles. Solid-phase extraction (SPE) optimization was accomplished by central composite design (CCD). In order to obtain the best results, the MCR-ALS model was compared with the parallel factor analysis 2 (PARAFAC2) model and validated by estimation of linearity, detection limits, and recovery.

Results
The detection limits and linear dynamics were calculated as 1.5, 4.27, and 4.77 µg/mL, and 1–50, 5–20, and 5–20 µg/mL for caffeine, ephedrine, and fluoxetine, respectively. Mean recovery for determination of caffeine, ephedrine, and fluoxetine using the developed method was reported as 101.75, 91.7, and 92.36, respectively.

Conclusion
The results showed that to avoid negative health outcomes associated with the excessive consumption of adulterated food supplements releasing such products should be carefully regulated. The developed method was validated using statistical factors and showed acceptable and reliable results.

Highlights
(1) The application of MCR-ALS coupled with HPLC-Diode-Array Detection data sets allowed the simultaneous identification and quantification of three key adulterants (caffeine, ephedrine, and fluoxetine) in dietary food supplements. (2) A small amount of the novel adsorbent was successfully used to preconcentrate the trace amounts of adulterants in samples. (3) This method benefits from the chemometrics tools and experimental design to significantly reduce the use of toxic solvents and complicated instruments to propose a less time-consuming method for quantification of multicomponents in the presence of uncalibrated interferents.
Speaker Responsibility for Synthetic Speech Derived from Neural Activity
Authors: Rainey Stephen 
Published: May 2022

Abstract
This article provides analysis of the mechanisms and outputs involved in language-use mediated by a neuroprosthetic device. It is motivated by the thought that users of speech neuroprostheses require sufficient control over what their devices externalize as synthetic speech if they are to be thought of as responsible for it, but that the nature of this control, and so the status of their responsibility, is not clear.
SN 2020acat: an energetic fast rising Type IIb supernova
Authors: Medler K , Mazzali P A , Teffs J , Ashall C , Anderson J P , Arcavi I , Benetti S , Bostroem K A , Burke J , Cai Y-Z , Charalampopoulos P , Elias-Rosa N , Ergon M , Galbany L , Gromadzki M , Hiramatsu D , Howell D A , Inserra C , Lundqvist P , McCully C , Müller-Bravo T , Newsome M , Nicholl M , Padilla Gonzalez E , Paraskeva E , Pastorello A , Pellegrino C , Pessi P J , Reguitti A , Reynolds T M , Roy R , Terreran G , Tomasella L , Young D R 
Published: May 2022

ABSTRACT
The ultraviolet (UV) and near-infrared (NIR) photometric and optical spectroscopic observations of SN 2020acat covering ∼250 d after explosion are presented here. Using the fast rising photometric observations, spanning from the UV to NIR wavelengths, a pseudo-bolometric light curve was constructed and compared to several other well-observed Type IIb supernovae (SNe IIb). SN 2020acat displayed a very short rise time reaching a peak luminosity of $\mathrm{{\rm Log}_{10}}(L) = 42.49 \pm 0.17 \, \mathrm{erg \, s^{-1}}$ in only ∼14.6 ± 0.3 d. From modelling of the pseudo-bolometric light curve, we estimated a total mass of 56Ni synthesized by SN 2020acat of MNi = 0.13 ± 0.03 M⊙, with an ejecta mass of Mej = 2.3 ± 0.4 M⊙ and a kinetic energy of Ek = 1.2 ± 0.3 × 1051 erg. The optical spectra of SN 2020acat display hydrogen signatures well into the transitional period (≳ 100 d), between the photospheric and the nebular phases. The spectra also display a strong feature around 4900  Å that cannot be solely accounted for by the presence of the Fe ii 5018 line. We suggest that the Fe ii feature was augmented by He i 5016 and possibly by the presence of N ii 5005. From both photometric and spectroscopic analysis, we inferred that the progenitor of SN 2020acat was an intermediate-mass compact star with an MZAMS of 15–20 M⊙.
Characterizing locus specific chromatin structure and dynamics with correlative conventional and super-resolution imaging in living cells
Authors: Mehra Dushyant , Adhikari Santosh , Banerjee Chiranjib , Puchner Elias M 
Published: May 2022

AbstractThe dynamic rearrangement of chromatin is critical for gene regulation, but mapping both the spatial organization of chromatin and its dynamics remains a challenge. Many structural conformations are too small to be resolved via conventional fluorescence microscopy and the long acquisition time of super-resolution photoactivated localization microscopy (PALM) precludes the structural characterization of chromatin below the optical diffraction limit in living cells due to chromatin motion. Here we develop a correlative conventional fluorescence and PALM imaging approach to quantitatively map time-averaged chromatin structure and dynamics below the optical diffraction limit in living cells. By assigning localizations to a locus as it moves, we reliably discriminate between bound and unbound dCas9 molecules, whose mobilities overlap. Our approach accounts for changes in DNA mobility and relates local chromatin motion to larger scale domain movement. In our experimental system, we show that compacted telomeres move faster and have a higher density of bound dCas9 molecules, but the relative motion of those molecules is more restricted than in less compacted telomeres. Correlative conventional and PALM imaging therefore improves the ability to analyze the mobility and time-averaged nanoscopic structural features of locus specific chromatin with single molecule sensitivity and yields unprecedented insights across length and time scales.
PiER: web-based facilities tailored for genetic target prioritisation harnessing human disease genetics, functional genomics and protein interactions
Authors: Fang Hai 
Published: May 2022

Abstract
Integrative prioritisation promotes translational use of disease genetic findings in target discovery. I report ‘PiER’ (http://www.genetictargets.com/PiER), web-based facilities that support ab initio and real-time genetic target prioritisation through integrative use of human disease genetics, functional genomics and protein interactions. By design, the PiER features two facilities: elementary and combinatory. The elementary facility is designed to perform specific tasks, including three online tools: eV2CG, utilising functional genomics to link disease-associated variants (particularly located at the non-coding genome) to core genes likely responsible for genetic associations in disease; eCG2PG, using knowledge of protein interactions to ‘network’ core genes and additional peripheral genes, producing a ranked list of core and peripheral genes; and eCrosstalk, exploiting the information of pathway-derived interactions to identify highly-ranked genes mediating crosstalk between molecular pathways. Each of elementary tasks giving results is sequentially piped to the next one. By chaining together elementary tasks, the combinatory facility automates genetics-led and network-based integrative prioritisation for genetic targets at the gene level (cTGene) and at the crosstalk level (cTCrosstalk). Together with a tutorial-like booklet describing instructions on how to use, the PiER facilities meet multi-tasking needs to accelerate computational translational medicine that leverages human disease genetics and genomics for early-stage target discovery and drug repurposing.
BioExcel Building Blocks Workflows (BioBB-Wfs), an integrated web-based platform for biomolecular simulations
Authors: Bayarri Genís , Andrio Pau , Hospital Adam , Orozco Modesto , Gelpí Josep Lluís 
Published: May 2022

Abstract
We present BioExcel Building Blocks Workflows, a web-based graphical user interface (GUI) offering access to a collection of transversal pre-configured biomolecular simulation workflows assembled with the BioExcel Building Blocks library. Available workflows include Molecular Dynamics setup, protein-ligand docking, trajectory analyses and small molecule parameterization. Workflows can be launched in the platform or downloaded to be run in the users’ own premises. Remote launching of long executions to user's available High-Performance computers is possible, only requiring configuration of the appropriate access credentials. The web-based graphical user interface offers a high level of interactivity, with integration with the NGL viewer to visualize and check 3D structures, MDsrv to visualize trajectories, and Plotly to explore 2D plots. The server requires no login but is recommended to store the users’ projects and manage sensitive information such as remote credentials. Private projects can be made public and shared with colleagues with a simple URL. The tool will help biomolecular simulation users with the most common and repetitive processes by means of a very intuitive and interactive graphical user interface. The server is accessible at https://mmb.irbbarcelona.org/biobb-wfs.
Sieving carbons promise practical anodes with extensible low-potential plateaus for sodium batteries
Authors: Li Qi , Liu Xiangsi , Tao Ying , Huang Jianxing , Zhang Jun , Yang Chunpeng , Zhang Yibo , Zhang Siwei , Jia Yiran , Lin Qiaowei , Xiang Yuxuan , Cheng Jun , Lv Wei , Kang Feiyu , Yang Yong , Yang Quan-Hong 
Published: May 2022

Abstract
Non-graphitic carbons are promising anode candidates for sodium-ion batteries, while their variable and complicated microstructure severely limits the rational design of high-energy carbon anodes that could accelerate the commercialization of sodium-ion batteries, as is the case for graphite in lithium-ion batteries. Here, we propose sieving carbons, featuring highly tunable nanopores with tightened pore entrances, as high-energy anodes with extensible and reversible low-potential plateaus (&lt;0.1 V). It is shown that the tightened pore entrance blocks the formation of the solid electrolyte interphase inside the nanopores and enables sodium clustering to produce the plateau. Theoretical and spectroscopic studies also show that creating a larger area of sodiophilic pore surface leads to an almost linearly increased number of sodium clusters, and controlling the pore body diameter guarantees the reversibility of sodium cluster formation, producing a sieving carbon anode with a record-high plateau capacity of 400 mAh g–1. More excitingly, this approach to preparing sieving carbons has the potential to be scalable for modifying different commercial porous carbons.
Disciplining interdisciplinarity: Infrastructure, identity, and interdisciplinary practice in nanoELSI research
Authors: Ku Sharon Tsai-hsuan , Zehr Stephen 
Published: May 2022

AbstractLarge-scale interdisciplinary collaboration between natural and social sciences has been advocated by funding agencies for enhancing communication between science and society. However, the actual infrastructure design and operation remain challenging, particularly for social-science-led interdisciplinary centers, which normally do not have core scientific facilities or centralized laboratories to coordinate collaborations among disciplines. Drawing upon ethnographic and interview data, this paper examines how the notion of interdisciplinarity was practiced in two federally-funded Centers for Nanotechnology in Society. We show how federal policies, university cultures, and local organizational structures significantly impacted forms of interdisciplinary practice and identity. In addition, we show that individuals’ interdisciplinary rhetoric, epistemic claims, and daily operation of interdisciplinarity require strong infrastructural support in terms of spatial and human resource arrangements to nurture cross-disciplinary coordination and trust as well as softening collaborative tensions while developing complementary projects.
Krüppel-like Factor 4 Supports the Expansion of Leukemia Stem Cells in MLL-AF9-driven Acute Myeloid Leukemia
Authors: Lewis Andrew Henry , Bridges Cory Seth , Moorshead David Neal , Chen Taylor J , Du Wa , Zorman Barry , Sumazin Pavel , Puppi Monica , Lacorazza H Daniel 
Published: May 2022

Abstract
Acute myeloid leukemia (AML) is an aggressive malignancy of the bone marrow with 5-year overall survival of less than 10% in patients over the age of 65. Limited progress has been made in the patient outcome because of the inability to selectively eradicate the leukemic stem cells (LSC) driving the refractory and relapsed disease. Herein, we investigated the role of the reprogramming factor KLF4 in AML because of its critical role in the self-renewal and stemness of embryonic and cancer stem cells. Using a conditional Cre-lox Klf4 deletion system and the MLL-AF9 retroviral mouse model, we demonstrated that loss-of-KLF4 does not significantly affect the induction of leukemia but markedly decreased the frequency of LSCs evaluated in limiting-dose transplantation studies. Loss of KLF4 in leukemic granulocyte-macrophage progenitors (L-GMP), a population enriched for AML LSCs, showed lessened clonogenicity and percentage in the G2/M phase of the cell cycle. RNAseq analysis of purified L-GMPs revealed decreased expression of stemness genes and MLL-target genes and upregulation of the RNA sensing helicase DDX58. However, silencing of DDX58 in KLF4 knockout leukemia indicated that DDX58 is not mediating this phenotype. CRISPR/Cas9 deletion of KLF4 in MOLM13 cell line and AML patient-derived xenograft cells showed impaired expansion in vitro and in vivo associated with a defective G2/M checkpoint. Collectively, our data suggest a mechanism in which KLF4 promotes leukemia progression by establishing a gene expression profile in AML LSCs supporting cell division and stemness.
Change of generations in the Austrian private foundation
Authors: Müller Katharina , Melzer Martin 
Published: May 2022

Abstract
In the Austrian private foundation, the status of founder cannot be acquired after the establishment of the foundation. The founder's genuine rights, such as the right to amend the foundation deed and the right to revoke the private foundation, can neither be transferred inter vivos nor upon death. Therefore, founders must ensure during their lifetime that the foundation deed is also tailored to the needs of the subsequent generations.
Aesthetic Limitations in Direct-to-Implant Prepectoral Breast Reconstruction
Authors: Safran Tyler , Al-Badarin Faris , Al-Halabi Becher , Viezel-Mathieu Alex , Dionisopoulos Tassos 
Published: May 2022

Background:
Prepectoral direct-to-implant reconstruction has become an alternative to staged subpectoral expander-based reconstruction. Although the surgical safety of this technique has been shown, aesthetic limitations have not been well-described. This article reports aesthetic limitations and elucidates risk factors that may predispose patients toward developing unfavorable outcomes following direct-to-implant prepectoral breast reconstruction.

Methods:
A retrospective chart review was performed, identifying patients who underwent prepectoral, direct-to-implant breast reconstruction from June of 2016 to June of 2019. Aesthetic limitations assessed included capsular contracture, rippling, implant malposition, and implant flipping.

Results:
Two hundred twenty-four consecutive women representing 334 breasts underwent immediate reconstruction performed by a single plastic surgeon. A midlateral incision was used in 185 breasts (55.4 percent) and the Wise pattern in 95 breasts (28.8 percent). The mean follow-up time was 30.5 months (45.3 to 18.3 months). Significant capsular contracture (grade 3 to 4) was noted in 27 breasts (8.1 percent), implant flipping in four breasts (1.2 percent), implant displacement in five breasts (1.5 percent), major rippling in nine breasts (2.7 percent), and minor rippling in 17 breasts (5.1 percent). The use of acellular dermal matrix had no significant effect on the aesthetic outcomes. In comparing breasts with postmastectomy radiation, there was a significant difference in the presence of minor rippling and capsular contracture (p < 0.05).

Conclusions:
This cohort represents the largest, single-surgeon, direct-to-implant prepectoral database in the literature. This report shows that aesthetic limitations were comparable to those seen with other forms of reconstruction. Complications did not differ in terms of acellular dermal matrix use. Certain factors can predispose patients to developing unfavorable aesthetic outcomes.

CLINICAL QUESTION/LEVEL OF EVIDENCE:
Therapeutic, IV.
Sexually Transmitted Infections Among Men and Transgender Women Using HIV Pre-exposure Prophylaxis in a Large Integrated Health System—A Cohort Study
Authors: Hechter Rulin C. , Bruxvoort Katia J. , Zhou Hui , Towner William J. , Contreras Richard , Schumacher Christina M. , Grant Deborah L. , Jones Joyce 
Published: May 2022

Background:
Sexually transmitted infections (STIs) are common in people using pre-exposure prophylaxis (PrEP). We examined risk and factors associated with STIs in a cohort of PrEP users in an integrated health system in the United States.

Setting:
The Kaiser Permanente Southern California is a large integrated health system that provides comprehensive medical services to approximately 4.7 million demographically diverse members.

Methods:
We identified men and transgender women initiating PrEP between January 1, 2014, and June 1, 2018, and followed through December 31, 2018. Demographic and clinical factors potentially associated with the risk of bacterial STIs during PrEP use were evaluated using Poisson regression models.

Results:
Among 5042 individuals tested for STIs with 7198 person‐years of follow-up, 1709 (33.9%) had at least one new STI. The estimated incidence of STIs was 48.3 per 100 person-years, and the most common STI was rectal chlamydia. Most repeat STIs (61.4%) occurred <180 days apart. In a multivariable analysis, an history of STIs in the prior 6 months through 7 days after the PrEP initiation was the most prominent risk factor of STIs during PrEP use (adjusted risk ratio: 1.78, 95% confidence intervals: 1.65 to 1.93). Other risk factors included younger age (<35 years), being Hispanic, and having a history of alcohol use disorder or drug use disorder.

Conclusions:
Quarterly STI testing and targeted intervention to mitigate STI risk are warranted for young and racial minority PrEP users, particularly for those with prior history of STIs and substance use disorders.
Prospects for probiotics in social bees
Authors: Motta Erick V. S. , Powell J. Elijah , Leonard Sean P. , Moran Nancy A. 
Published: May 2022

Social corbiculate bees are major pollinators. They have characteristic bacterial microbiomes associated with their hives and their guts. In honeybees and bumblebees, worker guts contain a microbiome composed of distinctive bacterial taxa shown to benefit hosts. These benefits include stimulating immune and metabolic pathways, digesting or detoxifying food, and defending against pathogens and parasites. Stressors including toxins and poor nutrition disrupt the microbiome and increase susceptibility to opportunistic pathogens. Administering probiotic bacterial strains may improve the health of individual bees and of hives, and several commercial probiotics are available for bees. However, evidence for probiotic benefits is lacking or mixed. Most bacterial species used in commercial probiotics are not native to bee guts. We present new experimental results showing that cultured strains of native bee gut bacteria colonize robustly while bacteria in a commercial probiotic do not establish in bee guts. A defined community of native bee gut bacteria resembles unperturbed native gut communities in its activation of genes for immunity and metabolism in worker bees. Although many questions remain unanswered, the development of natural probiotics for honeybees, or for commercially managed bumblebees, is a promising direction for protecting the health of managed bee colonies.
This article is part of the theme issue ‘Natural processes influencing pollinator health: from chemistry to landscapes’.
Pollinator nutrition and its role in merging the dual objectives of pollinator health and optimal crop production
Authors: Jones Jeremy , Rader Romina 
Published: May 2022

Bee and non-bee insect pollinators play an integral role in the quantity and quality of production for many food crops, yet there is growing evidence that nutritional challenges to pollinators in agricultural landscapes are an important factor in the reduction of pollinator populations worldwide. Schemes to enhance crop pollinator health have historically focused on floral resource plantings aimed at increasing pollinator abundance and diversity by providing more foraging opportunities for bees. These efforts have demonstrated that improvements in bee diversity and abundance are achievable; however, goals of increasing crop pollination outcomes via these interventions are not consistently met. To support pollinator health and crop pollination outcomes in tandem, habitat enhancements must be tailored to meet the life-history needs of specific crop pollinators, including non-bees. This will require greater understanding of the nutritional demands of these taxa together with the supply of floral and non-floral food resources and how these interact in cropping environments. Understanding the mechanisms underlying crop pollination and pollinator health in unison across a range of taxa is clearly a win–win for industry and conservation, yet achievement of these goals will require new knowledge and novel, targeted methods.
This article is part of the theme issue ‘Natural processes influencing pollinator health: from chemistry to landscapes’.