bluuebunny/crossref_metadata_2025_split huggingface dataset:
The accuracy of nucleic acid amplification tests (NAATs) in detecting human intestinal nematode infections: A protocol for a systematic review and meta-analysis
Authors: Jayakody Nalini Kaushalya , Kumbukgahadeniya Poornima Lakshini , Silva Anjana , Wickramasinghe Nuwan Darshana , Wickramasinghe Susiji , McManus Donald Peter , Weerakoon Kosala Gayan 
Published: December 2022

Human intestinal nematode infections are a global public health issue as they can result in considerable morbidity in infected individuals, mainly in developing countries. These infections continue to go undiagnosed, as they tend to be mainly endemic in resource-poor communities where there is a shortage of experienced laboratory staff and relevant diagnostic technologies. This is further exacerbated by the nature of intermittent shedding of eggs and larvae by these parasites. Diagnostic methods range from simple morphological identification to more specialised high-throughput sequencing technologies. Microscopy-based methods, although simple, are labour-intensive and considerably less sensitive than molecular methods which are rapid and have high levels of accuracy. Molecular methods use nucleic acid amplification (NAA) to amplify the deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) fragments of the parasite to detect and determine its presence using different technologies (NAAT). They have increased the sensitivity of detection and quantitation of intestinal nematode infections, especially in low infection intensity settings. The absence of a gold standard test limits current diagnosis and, in turn, restricts intervention measures and effective control efforts. The objective of this review is to determine the accuracy of NAATs in detecting human intestinal nematode infections using Kato-Katz as the reference test for the most common soil-transmitted helminth (STH) infections and the scotch tape test for enterobiasis and Baermann method for strongyloidiasis. Relevant studies will be identified by searches in electronic databases. Two reviewers will independently screen the literature against eligibility criteria. The methodological quality of studies will then be appraised by two reviewers using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. Discrepancies will be addressed by a third reviewer. The true positives, false positives, true negatives and false negatives of all the studies will be extracted into contingency tables. In paired forest plots, study-specific sensitivity and specificity with a 95 per cent confidence interval will be displayed. The systematic review of this protocol will report the diagnostic accuracy of currently available NAATs for the detection of human intestinal nematode infections. This will help healthcare providers and administrators determine the diagnostic method to be used in different clinical and preventive settings.
Trial registration: PROSPERO registration number for this protocol is CRD42022315730.
GIS, GPS AND REMOTE SENSING APPLICATION TO INVESTIGATE AGRICULTURAL POTENTIAL IN CHOLISTAN
Authors: Ahmad Farooq 
Published: December 2022

Agriculture plays the most important part in the economy of our country from macro perspective,
while an equally important part in our social setup from a micro perspective. The ecological
system of Cholistan desert is highly delicate and has gone under tremendous changes brought
upon by physical and mainly by human interventions. The rapid development and integration
of spatial technologies such as Geographic Information System, Global Positioning System,
and Remote Sensing, have created many new tools for professionals, but have also widened
the "digital divide", leaving many with little understanding of the technology and potential
applications. Remote sensing can provide valuable, timely and even predicted information
about environment as an important basis for sustainable development. Geographic Information
System can provide effective tools for decision makers. The uses of GIS, GPS, and RS technologies,
either individually or in combination, span a broad range of applications and degrees
of complexity. It facilitates cooperative approaches and with the integration of GIS, there will
be a whole new framework for organizations and society to work together and make decisions.
However, an integrated GIS based database management system handles this highly significant
task to make our country more prosper among Nations.
Introducing Construction Semantics (CxS): a frame-semantic extension of Construction Grammar and constructicography
Authors: Willich Alexander 
Published: December 2022

Abstract
Construction Semantics (CxS) uses FrameNet frames to capture the semantic properties of grammatical constructions. It closes a gap between Frame Semantics and usage-based Construction Grammar as well as providing a uniform format to implement frames into constructicographic work. This article provides an overview of three core features of CxS. Starting point is (i) the modeling of semantic properties associated with constructions in terms of three types of frames. This makes possible (ii) the analysis of structural parallels between constructions and frames, in particular the semantic motivation of elements of a construct by frame elements. Lastly, (iii) an inventory of semantic parameters of constructions serves as a toolkit for generalizing over semantic properties of single constructs. A driving force behind CxS is the concept of frame proximity: a network of frames which allows one to distinguish lexically evoked frames that are related or unrelated to a frame associated with a construction.
Thermal transport with nanoparticles of fractional Oldroyd-B fluid under the effects of magnetic field, radiations, and viscous dissipation: Entropy generation; via finite difference method
Authors: Asjad Muhammad Imran , Usman Muhammad , Kaleem Muhammad Madssar , Baleanu Dumitru , Muhammad Taseer 
Published: December 2022

Abstract
It is a well-known fact that functional effects like relaxation and retardation of materials, and heat transfer phenomena occur in a wide range of industrial and engineering problems. In this context, a mathematical model is developed in the view of Caputo fractional derivative for Oldroyd-B nano-fluid. Nano-sized particles of copper (Cu) are used to prepare nano-fluid taking water as the base fluid. The coupled non-linear governing equations of the problem are transformed into dimensionless form. Finite difference scheme is developed and applied successfully to get the numerical solutions of deliberated problem. Influence of different physical parameters on fluid velocity profile and temperature profile are analyzed briefly. It is observed that for increasing values of fractional parameter (α), fluid velocity increased, but opposite behavior was noticed for temperature profile. Nusselt number (Nu) decayed for advancement in values of heat source/sink parameter (Q
0), radiation parameter (Nr), volume fraction parameter of nano-fluid (ϕ), and viscous dissipation parameter (Ec). Skin friction (C
f) boosts for the increase in the values of magnetic field parameter (Ha). It can also be noticed that the extended finite difference scheme is an efficient tool and gives the accurate results of discussed problem. It can be extended for more numerous type heat transfer problems arising in physical nature with complex geometry.
The treatment lottery of chronic back pain? A case series at a multidisciplinary pain centre
Authors: Mattsson Anna , Ghafouri Nazdar , Bäckryd Emmanuel 
Published: December 2022

Abstract

Objectives
Despite the number of people affected by chronic back pain, and the many available treatment options, even the best modalities provide limited pain reduction on a group level, often without simultaneous improvements in functioning or health-related quality of life. The objective was to provide an overview of the treatment of chronic back pain in clinical practice at a multidisciplinary pain centre, and to study patient and pain characteristics in different treatment groups.

Methods
104 chronic back pain patients (primary ICD-10-SE-diagnosis M53.0–M54.9 excluding M54.1 and M54.3), referred to the Pain and Rehabilitation Centre, University Hospital, Linköping in 2015, were studied using data from the Swedish Quality Registry for Pain Rehabilitation, self-reported medication data, and a retrospective medical record review.

Results
The following treatment groups were identified: rehabilitation (n=21), analgesics (n=33), invasive intervention (n=14), and no treatment (n=35). Significant differences between groups were found with regards to age, sick leave, education level, persisting pain duration, punishing responses by significant other, previous invasive intervention, receiving sub-clinic, physician speciality and referring care level.

Conclusions
Overall, patient demographics were associated with treatment strategy to a higher degree than patient-reported outcome measures. Moreover, physician speciality and organisational factors seemed to play a role in treatment choice.
Emotional Memory Processing during REM Sleep with Implications for Post-Traumatic Stress Disorder
Authors: Rho Young-Ah , Sherfey Jason , Vijayan Sujith 
Published: December 2022

REM sleep is important for the processing of emotional memories, including fear memories. Rhythmic interactions, especially in the theta band, between the medial prefrontal cortex (mPFC) and limbic structures are thought to play an important role, but the ways in which memory processing occurs at a mechanistic and circuits level are largely unknown. To investigate how rhythmic interactions lead to fear extinction during REM sleep, we used a biophysically based model that included the infralimbic cortex (IL), a part of the mPFC with a critical role in suppressing fear memories. Theta frequency (4–12 Hz) inputs to a given cell assembly in IL, representing an emotional memory, resulted in the strengthening of connections from the IL to the amygdala and the weakening of connections from the amygdala to the IL, resulting in the suppression of the activity of fear expression cells for the associated memory. Lower frequency (4 Hz) theta inputs effected these changes over a wider range of input strengths. In contrast, inputs at other frequencies were ineffective at causing these synaptic changes and did not suppress fear memories. Under post-traumatic stress disorder (PTSD) REM sleep conditions, rhythmic activity dissipated, and 4 Hz theta inputs to IL were ineffective, but higher-frequency (10 Hz) theta inputs to IL induced changes similar to those seen with 4 Hz inputs under normal REM sleep conditions, resulting in the suppression of fear expression cells. These results suggest why PTSD patients may repeatedly experience the same emotionally charged dreams and suggest potential neuromodulatory therapies for the amelioration of PTSD symptoms.SIGNIFICANCE STATEMENTRhythmic interactions in the theta band between the mPFC and limbic structures are thought to play an important role in processing emotional memories, including fear memories, during REM sleep. The infralimbic cortex (IL) in the mPFC is thought to play a critical role in suppressing fear memories. We show that theta inputs to the IL, unlike other frequency inputs, are effective in producing synaptic changes that suppress the activity of fear expression cells associated with a given memory. Under PTSD REM sleep conditions, lower-frequency (4 Hz) theta inputs to the IL do not suppress the activity of fear expression cells associated with the given memory but, surprisingly, 10 Hz inputs do. These results suggest potential neuromodulatory therapies for PTSD.
Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis
Authors: Islam Md Saiful , Hardwick Steven W , Quell Laura , Durica‐Mitic Svetlana , Chirgadze Dimitri Y , Görke Boris , Luisi Ben F 
Published: December 2022

AbstractBiogenesis of the essential precursor of the bacterial cell envelope, glucosamine‐6‐phosphate (GlcN6P), is controlled by intricate post‐transcriptional networks mediated by GlmZ, a small regulatory RNA (sRNA). GlmZ stimulates translation of the mRNA encoding GlcN6P synthtase in Escherichia coli, but when bound by RapZ protein, the sRNA becomes inactivated through cleavage by the endoribonuclease RNase E. Here, we report the cryoEM structure of the RapZ:GlmZ complex, revealing a complementary match of the RapZ tetrameric quaternary structure to structural repeats in the sRNA. The nucleic acid is contacted by RapZ mostly through a highly conserved domain that shares an evolutionary relationship with phosphofructokinase and suggests links between metabolism and riboregulation. We also present the structure of a precleavage intermediate formed between the binary RapZ:GlmZ complex and RNase E that reveals how GlmZ is presented and recognised by the enzyme. The structures provide a framework for understanding how other encounter complexes might guide recognition and action of endoribonucleases on target transcripts, and how structured substrates in polycistronic precursors may be recognised for processing by RNase E.
Immersion between Semiotic Theories
Authors: Melnikova Irina 
Published: December 2022

The paper focuses on the book of poetry immersion (2017) by Lithuanian poet Gytis Norvilas, maps the strategies of its reading within the framework of the semiotic-pragmatic approach, and considers the theoretical issues of the mapping. Despite the book cannot be unambiguously defined as an example of patterned poetry, it is configured in a specific way which exposes the functionality of the structural visual layer in written / printed poetry. This feature of the text in question inspires to revise the interpretation of the issue of iconicity and the configuration of the model of reading literature, proposed by the semiotic-pragmatic approach. The semiotic-pragmatic modelling combines Peircean and Jakobsonian ideas with minor insertions of Lotmanian thought (i.e. Johansen 2002), thus it links the ideas from the conceptual systems, based on different epistemological grounds (Peircean vs Saussurean notion of sign). It creates theoretical tensions that force to raise the question of how the transference of notions from one system to another changes their meaning, and where one could find the basis for overcoming or bypassing the controversies. The first part of the paper explores the theoretical tensions between Peircean, Jakobsonian and Lotmanian approaches to the issues of iconicity and attempts at revealing the fields of theoretical reasoning that help to define the ground of contradictions and see the way of their bypassing. Besides, it relates the discussion of iconicity with a discussion of the semiotic-pragmatic model of reading as iconization, based on the Jakobsonian idea of intersemiotic translation, and revises the model concerning the printed poetic text. The second part of the paper exemplifies the theoretical issues in the analysis of the book of poetry immersion. It examines the process of diagrammatization – the reading strategy, which makes the syntax of the text and that of the book the meaning-making devices, shows how diagrammatization semantizes the structure of the book, transforms the process of imaginization, and helps to identify the role of the book’s dialogue with hypotext to which both intertextual references and the structure of the text refer.
Upper tract urothelial carcinoma: can the tumor be removed en bloc?
Authors: Martov A. G. , Ergakov D. V. , Andronov A. S. 
Published: December 2022

Diagnosis and treatment of upper tract urothelial carcinoma (UTUC) remain important problems of clinical urology. UTUC en bloc i.e. in one-piece removal during organ-sparing surgery is still hardly possible. The most typical endourological operations are either electrosurgery, holmium or thulium YAG laser tumor vaporization.We present a clinical case of ureteroscopic en bloc UTUC removal using a new super pulse thulium fiber laser (SPTFL, FiberLase U3). Recent innovations and trends in this field are also discussed. The technical steps of en bloc ureteroscopic tumor removal allowing for diagnosis verification, identification of pT stage and surgical margin are described. The further innovations in laser UTUC removal include modulation of laser pulse length and development of the correlation between preoperative tumor characteristics and possibility of en bloc removal.
Cytotoxic and antitumor properties of methionine γ-lyase conjugate in combination with S-alk(en)yl–L-cysteine sulfoxides
Authors: Abo Qoura L. , Morozova E. A. , Koval V. S. , Kulikova V. V. , Spirina T. S. , Demidova E. A. , Demidkina T. V. , Pokrovsky V. S. 
Published: December 2022

Background. Enzyme prodrug therapy is a promising strategy to treat solid malignancies. The utilization of two-component systems, including an enzyme and a non-toxic prodrug, to generate cytotoxic compounds directly at the surface of the tumor cell can be successful strategy in reducing the overall toxic load on the body.Aim. To determine antitumor activity of the pharmacological pair C115H methionine γ-lyase (C115H MGL) conjugated with daidzein (C115H MGL-Dz) and of S-alk(en)yl-L-cysteine sulfoxides against various types of solid tumors in vitro and in vivo.Materials. MTT-test was used to determine the cytotoxicity of C115H MGL-Dz in the presence of S-alk(en)yl-L-cysteine sulfoxides in vitro against Sw620 (colon cancer), Panc1 (pancreatic cancer), and 22Rv1 (prostate cancer). Apopto- sis induction and cell cycle alteration in 22Rv1, Sw620, and SKBR3 cell lines were studied using the Muse® Caspase-3/7 and Muse® Cell Cycle Assay kits. In vivo anticancer activity was studied on Sw620, Panc1, and 22Rv1 subcutaneous xenografts in Balb/c nude mice.Results. The C115H MGL-Dz had the maximum cytotoxic activity in the presence of S-propyl-L-cysteine sulfoxide (propiin) with IC50 values: 3.88 and 5.4 for Panc1 and 22Rv1, respectively. Dipropyl thiosulfinate formed by the β-eli-mination of propiin catalyzed by C115H MGL-Dz, induces apoptosis through both the activation of caspases and alternative pathways, and also it inhibits cell division, contributing to a decrease in the concentration of cells in the G2/M phase. The anticancer efficacy of the pharmacological pair C115H-Dz/propiin in vivo indicated a significant decrease in Panc1 tumor volume (tumor growth inhibition (TGI) 67.5 %, p = 0.004), Sw620 (TGI 22.07 %, p = 0.12) and 22Rv1 (TGI 70 %, p = 0.043).Conclusion. Pharmacological pair C115H MGL-Dz/propiin was capable of suppressing tumor development in malignant solid tumors and might be considered as a potential anticancer approach in cancer prodrug therapy.
Effect of LHS-1269 on morphology and angiogenesis of Lewis lung carcinoma
Authors: Merkulova I. B. , Golubeva I. S. , Khochenkov D. А. , Abramova T. V. , Ermakova N. P. , Ektova L. V. 
Published: December 2022

Background. The new anticancer chemical compound LHS-1269 from the class of indolocarbazoles is undergoing preclinical studies at the N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia. LHS-1269 has a high antitumor activity on transplanted tumors of mice, showed high cytotoxic activity on many tumor cells lines in vitro, and has shown an inhibitory effect on vasculogenic mimicry in tumors in vitro. LHS-1269 does not affect the catalytic activity of human topoisomerases I and IIα. An antiangiogenic mechanism of the drug’s antitumor action is suggested.Aim. To evaluate the effect of LHS-1269 on the morphological features and angiogenesis of transplanted Lewis lung carcinoma (LLC) in BDF1 mice.Materials and methods. In BDF1 mice (n = 20) with transplanted LLC tumor on days 1 and 3 after 5-fold intraperitoneal administration of LHS-1269 in a single dose of 60 mg (total dose – 300 mg/kg), mice (n = 20) on days 5 and 8 after a single intravenous injection of LHS-1269 at a dose of 100 mg/kg, mice (n = 20) with transplanted LLC tumor without administration of LHS-1269 (control). The assessment of the antitumor effect was carried out according to the criterion of inhibition of tumor growth (%) and the study of the morphological features of the tumors. To assess the effect of LHS-1269 on tumor angiogenesis in LLC tumor sections, a visual calculation of the average number (density) of blood vessels and immunohistochemical detection of expression of the CD31+ endothelial marker were performed.Results. The LHS-1269 compound in animal groups with 5-fold and 1-fold use caused tumor growth inhibition – 59–70 and 67–79 %, with pronounced morphological changes and tumor cell death. There is an uneven distribution of blood vessels in tumors and surrounding tissues in all groups. LHS-1269 caused a statistically significant decrease in the average number of blood vessels in the tumor both after 5-fold and after 1-fold administration. The statistically significant decrease in the average number of blood vessels in the surrounding connective tissue tumor was observed only after 5-fold administration of the drug. The decrease in the average number of CD31+ endothelial cells after five times intraperitoneal administration was statistically insignificant compared to control (83.1 ± 8.7 and 59.6 ± 18.9, respectively). The increase in this indicator after a single intravenous injection of LHS-1269 was statistically significant.Conclusion. LHS-1269, when administered five times and once in mice, caused pronounced morphological changes in the form of damage and death of LLC tumor cells. The results of a study of angiogenesis in a tumor do not allow an unambiguous conclusion about the inhibitory effect of LHS-1269 on angiogenesis in LLC tumors in mice.
Sarcoidosis: federal clinical guidelines for diagnosis and treatment
Authors: Chuchalin A. G. , Avdeev S. N. , Aisanov Z. R. , Baranova O. P. , Borisov S. E. , Geppe N. A. , Vizel’ A. A. , Vizel’ I. Yu. , Zaicev A. A. , Kravchenko N. Y. , Ilkovich M. M. , Lovacheva O. V. , Malakhov A. B. , Malyavin A. G. , Petrov D. V. , Romanov V. V. , Sivokozov I. V. , Samsonova M. V. , Solovieva I. P. , Stepanyan I. E. , Terpigorev S. A. , Tyurin I. E. , Frantsuzevich L. Ya. , Chernyaev A. L. , Shmelev E. I. , Shmeleva N. M. 
Published: December 2022

Sarcoidosis is a multi-organ granulomatosis of unknown origin. Modern diagnostic methods allow detecting this disease at an early stage. The absence of specific markers requires a comprehensive approach to diagnosis based on comparison of radiation, clinical, morphological and functional data. The course of sarcoidosis without damage to the respiratory system presents significant difficulties. It is extremely important to understand the time and means of starting Sarcoidosis’s treatment to avoid the early initiation of hormones and cytostatics and, on the other hand, to timely respond to progression and threatening conditions. Methods. Clinical recommendations are based on the analysis of Russian and English publications of the latest sarcoidosis research. The target audience of these clinical guidelines are therapists, general practitioners, pulmonologists, TB doctors, rheumatologists, dermatologists, radiation diagnosticians, immunologists, and clinical pharmacologists. Each thesis-recommendation for diagnosis and treatment is evaluated on an 1 to 5 scale of levels of evidence and an A, B, C scale of the grades of recommendations. The clinical guidelines also contain comments and explanations for the theses-recommendations, diagnostic algorithms, treatment strategies, reference materials on the use of recommended drugs. Conclusion. Current information on epidemiology, clinical manifestations, diagnosis and management strategies for patients with sarcoidosis are covered in the presented clinical guidelines. Approved by the decision of the Scientific and Practical Council of the Ministry of Health of the Russian Federation (2022).