bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA sequencing analyses of gene expression by CRISPR/Cas9 knockout of CLL-1 gene in acute myeloid leukemia cells
Authors: Wang Yanyu , Wu Songfang , Yang Jing , Wang ShiLiang , Xiong Hong , Li Shui-Jun 
Published: November 2022

AbstractCLL-1 has been revealed its potential role in acute myeloid leukemia (AML), however, the underlying mechanisms remain unclear. CRISPR/Cas9 strategy was employed to knock out CLL-1 gene in U937 cells and western-blot was used to validate the success of knock out. CCK8 and Transwell assays were used to detect cells viability and migration, respectively. RNA-sequencing was performed to profile mRNA expression in CLL-1 gene knock-out and wide type U937 cells. A cutoff of 1.5-fold change and false discovery rate (FDR) <0.05 was used to screen differentially expressed genes (DEGs), which were presented by volcano plots and hierarchical cluster heatmap. Protein-protein interaction (PPI) network was constructed by String database and Cytoscape software. Furthermore, hub genes were mined by CytoNCA and MCODE, which were subjected to functional enrichment using R package. Finally, the findings were validated using qRT-PCR and western-blot. The protein level of CLL-1 was significantly lowered, and cell viability and migration were suppressed in knock-out cells compared to wide type. Using RNA-sequencing and bioinformatics analysis, 452 DEGs (179 up-regulated and 273 down-regulated) were obtained, and several important hub genes (such as CCR2, FBXO21, UBB and UBE2C) were filtered out, which were enriched in 132 GO terms and 36 KEGG pathways such as chemokine signaling pathway and ubiquitin mediated proteolysis. A total of 8 representative genes mRNA expressions were validated by qRT-PCR, and the protein levels of 6 genes were confirmed by western-blot. CLL-1 gene might exert its role in AML through modulating genes enriched in multiple functions such as chemokine signaling and ubiquitination. Our results may give us new knowledge of CLL-1 in AML and provide a basis for mining novel targets.
Profiling of chimeric RNAs in human retinal development with retinal organoids
Authors: Wang Wen , Zhang Xiao , Zhao Ning , Xu Ze-Hua , Jin Kangxin , Jin Zi-Bing 
Published: November 2022

AbstractChimeric RNAs have been found in both cancer and healthy human cells. They have regulatory effects on human stem/progenitor cell differentiation, stemness maintenance and central nervous system (CNS) development. However, their physiological functions in the retinal development remain unknown. Based on the human embryonic stem cells (hESC)-derived retinal organoids (ROs) spanning from day 0 to day 120, we present the expression atlas of chimeric RNAs throughout the developing ROs. We confirmed the existence of some common chimeric RNAs and also discovered many novel chimeric RNAs during retinal development. We focused on CTNNBIP1-CLSTN1 (CTCL) whose downregulation causes precocious neuronal differentiation and a marked reduction of neural progenitors in human cerebral organoids. Our study found that CTCL also plays a key role in human retinogenesis, CTCL loss-of-function obstructed RO differentiation but prompted the retinal pigment epithelial (RPE) differentiation. Together, this work provides a landscape of chimeric RNAs and reveals evidence for their crucial roles in human retina development.
Cotranslational formation of disulfides guides folding of the SARS COV-2 receptor binding domain
Authors: Bitran Amir , Park Kibum , Serebryany Eugene , Shakhnovich Eugene I. 
Published: November 2022

AbstractMany secreted proteins contain multiple disulfide bonds. How disulfide formation is coupled to protein folding in the cell remains poorly understood at the molecular level. Here, we combine experiment and simulation to address this question as it pertains to the SARS-CoV-2 receptor binding domain (RBD). We show that, whereas RBD can refold reversibly when its disulfides are intact, their disruption causes misfolding into a nonnative molten-globule state that is highly prone to aggregation and disulfide scrambling. Thus, non-equilibrium mechanisms are needed to ensure disulfides form prior to folding in vivo. Our simulations suggest that co-translational folding may accomplish this, as native disulfide pairs are predicted to form with high probability at intermediate lengths, ultimately committing the RBD to its metastable native state and circumventing nonnative intermediates. This detailed molecular picture of the RBD folding landscape may shed light on SARS-CoV-2 pathology and molecular constraints governing SARS-CoV-2 evolution.
A metabolome-wide Mendelian randomization study prioritizes causal circulating metabolites for multiple sclerosis
Authors: Ge Angela , Sun Yitang , Kiker Thaddaeus , Zhou Yanjiao , Ye Kaixiong 
Published: November 2022

AbstractObjectiveTo prioritize circulating metabolites that likely play causal roles in the development of multiple sclerosis (MS).MethodsTwo-sample Mendelian randomization analysis was performed to estimate the causal effects of 571 circulating metabolites on the risk of MS. Genetic instruments for circulating metabolites were obtained from three previous genome-wide association studies (GWAS) of the blood metabolome, while genetic associations with MS were from a large GWAS by the International Multiple Sclerosis Genetics Consortium. The primary analysis was performed with the multiplicative random-effect inverse variance-weighted method, while multiple sensitivity analyses were conducted with the weighted median, weighted mode, MR-Egger, and MR-PRESSO.ResultsA total of 29 metabolites had suggestive evidence of causal associations with MS. Genetically instrumented levels of serine (OR = 1.56, 95% CI = 1.25 – 1.95), lysine (OR = 1.18, 95% CI = 1.01 – 1.38), acetone (OR = 2.45, 95% CI = 1.02 – 5.90), and acetoacetate (OR = 2.47, 95% CI = 1.14 – 5.34) were associated with a higher MS risk. Total cholesterol and phospholipids in large very-low-density lipoprotein were associated with a lower MS risk (OR = 0.83, 95% CI = 0.69 – 1.00; OR = 0.80, 95% CI = 0.68 – 0.95), but risk-increasing associations (OR = 1.20, 95% CI = 1.04 – 1.40; OR = 1.13, 95% CI = 1.00 – 1.28) were observed for the same two lipids in very large high-density lipoprotein.ConclusionsOur metabolome-wide Mendelian randomization study prioritized a short list of circulating metabolites, such as serine, lysine, acetone, acetoacetate, and lipids, that likely have causal associations with MS.
Differences between robot servers and human servers in brand modernity, brand love and behavioral intentions in the restaurant industry
Authors: Kim Heather Markham , Abbas Jawad , Haq Muhammad Zia Ul , Lee JungHoon (Jay) , Hwang Jinsoo 
Published: November 2022

PurposeThis study examined the effect of brand modernity on brand love. In addition, this study investigated how brand love affects behavioral intentions including intentions to use, word-of-mouth and willingness to pay more. Lastly, this study explored the differences between robot servers and human servers in the five concepts mentioned above.Design/methodology/approachData were collected from 300 people who experienced robot servers and from 318 people who experienced human servers.FindingsThe results of data analysis revealed that brand modernity has a positive influence on brand love, which in turn positively affects intentions to use, word-of-mouth and willingness to pay more. Additionally, it was found that there were statistical differences with the mean value of the five concepts based on the type of employee, such as robot servers and human servers.Originality/valueUnlike prior research, the difference between robot servers and human servers was investigated by focusing more on the brand in this study, and such findings can be considered as important theoretical implications of this study.
Trade barrier decrease and environmental pollution improvement: new evidence from China’s firm-level pollution data
Authors: Chen Dengke 
Published: November 2022

PurposeTrade and environment are essential issues closely related to the development of the national economy and the improvement of people’s livelihood in the new era. The Report to the 19th National Congress of the Communist Party of China (CPC) listed the construction of a strong trading power as an important part of building a modern economic system and pollution prevention and treatment as one of the three key battles to win the decisive victory of building a moderately prosperous society in all respects. However, the relationship between trade and environmental pollution is still very controversial in the existing literature, and there is a paucity of literature on the relationship between trade and environmental pollution based on micro data.Design/methodology/approachThis paper merged China’s Firm-Level Pollution Database with China’s Industrial Enterprise Database and China’s industry tariff rates. Additionally, by virtue of the quasi-natural experiment of China’s accession to the World Trade Organization (WTO), a difference in difference (DID) model was constructed to alleviate the endogeneity issue.FindingsAccording to the results, the trade barrier decrease (trade liberalization) significantly reduces the intensity of SO2 emissions, a major pollutant of enterprises, as the intensity of SO2 emissions decreased 2.16% for each unit decrease of the trade barrier. The analysis of the mechanisms shows that the SO2 emission intensity of enterprises is mainly due to the decrease of enterprises’ pollution emission rather than the decrease of output, and the decrease of enterprises’ pollution emission is mainly caused by the enterprises’ cleaner production process rather than the end treatment of pollution emission. The decrease of coal use intensity is an important mechanism of the decrease of SO2 emission intensity caused by the decrease of trade barriers. Among the technical effects of the change of the trade barrier affecting enterprises’ pollution emission, biased technical change rather than neutral technical change dominates.Originality/valueThe findings of this paper imply that expanding openness can enhance China’s social welfare not only through the economic growth mechanisms identified in the classical literature, but also through environmental improvements. This provides useful policy insights for promoting the construction of a strong trading power and winning the battle against pollution in the new era.
On the theoretical source of the reform of socialist open economy with Chinese characteristics
Authors: Lu Jiang , Zhang Chen 
Published: November 2022

PurposeSome economic theories have influenced the reform of the socialist open economy with Chinese characteristics. As a new practice of socialism, an open economy is not only driven by China’s productivity level and people’s living standards but also regulated by the law of commodity production and value.Design/methodology/approachIt was popular to participate in economic globalization for most countries in the second half of the 20th century, but not all of them could benefit from it.FindingsThe key to the success of the open-economy reform with Chinese characteristics lies in learning from and innovating the comparative advantage theory, thus forming an organic whole of the open economy, including the core of correctly handling the relationship between the government and the market, the method of gradual reform, the breakthrough point of transforming the mode of economic development, and serving people all the time.Originality/valueAchieving internal and external coordination through the combination of opening-up and independence is a critical principle of China’s economic opening-up, which not only effectively safeguards national interests but also actively promotes the construction of a new global order.
Ownership, trickle-down effect and shared development: a political economy analysis
Authors: Gai Kaicheng , Zhou Yongsheng 
Published: November 2022

PurposeAs an essential part of mainstream Western development economics, the trickle-down theory originates from the behavioral choices and iterations of thought on conflicts of interest in the evolution of remuneration structure in Western countries. The fundamental flaw of the logic of this theory is that it conceals the inherent implication of social systems and the essential characteristics of social structures.Design/methodology/approachThis paper examines the relationships among economic growth, income distribution and poverty from the perspective of social relations of production – the nature of production relations determines the nature of distribution relations and further determines the essence of trickle-down development, and ownership is the core mechanism for realizing the trickle-down effect.FindingsThe stagnation or smoothness of the trickle-down effect in different economies is essentially subject to the logic of “development for whom”, which is determined by ownership relationship.Originality/valueTo be more specific, “development for capitalists” and “development for the people” indicate two distinctly different economic growth paths. The former starts with private ownership and follows a bottom-up negative trickle-down path that inevitably leads to polarization, while the latter starts with public ownership and follows a top-down positive trickle-down path that will lead to common prosperity in the end.
UPMRC: rewriting the success story of Lucknow Metro during Covid-19 crisis
Authors: Saxena Anupam , Tripathi Shalini Nath , Singh Swadesh Kumar 
Published: November 2022

Learning outcomes
After working through the case and assignment questions, students will be able to understand the following aspects:▪ how good strategic planning can convert a crisis into an opportunity;▪ importance of service excellence and customer satisfaction through customer delight and customer feedback; and▪ utilisation of resources and excellent time management strategies.

Case overview/synopsis
This case discusses how vital teamwork and motivated leadership can convert a crisis such as Covid-19 into an opportunity. This case study talks about Uttar Pradesh Metro Rail Corporation (UPMRC), a metro rail corporation working to develop metro trains in the Indian state of Uttar Pradesh. The case discusses how challenging it was for the metro rail corporation to transform its processes in a short period and deal with the crisis on major fronts such as facilities maintenance, human resource management, ensuring safety and security of its staff and riders, motivation of staff, service quality and maintaining all operational aspects. The case discusses how UPMRC is a leader on all fronts and has excelled in its operational work. It talks about what challenges the lockdown and unlocking phase posed in front of the leadership and how teamwork, dedication to exemplary service quality and customer satisfaction gave the team the strength to make changes that improved their processes and helped them overcome the crisis.The case starts with a discussion of metro rail inception and incorporation of UPMRC and then how this newly formed metro has to face the challenges of pre-lockdown period where the team worked very hard for sanitisation and safety. The lockdown created a completely different set of challenges related to the facilities and the entire metro train systems, which was a difficult situation to deal because of restrictions and other challenges. However, the team dealt with situations with strength and strategic planning, leading to better managed processes and staff. The unlocking phase also gave many challenges that the team handled with a lot of care and efficiency.

Complexity academic level
This case is suitable for post-graduate-level courses on services marketing, service operations management, general management, crisis management and strategic management. Participants can use the case to develop an understanding of strategic planning and management.This case can also be used in the executive education program for managers to encourage them to think through challenges faced by metro rail corporations.

Supplementary materials
Teaching notes are available for educators only.

Subject Code
CSS 10: Public Sector Management.
A new digital literacy framework to mitigate misinformation in social media infodemic
Authors: Anthonysamy Lilian , Sivakumar Pravina 
Published: November 2022

Purpose
This study aims to examine how digital literacy competency can mitigate misinformation in social media among young adults. In recent years, concerns over misinformation have triggered a renewed interest in the aspect of digital literacy. Many young adults in Malaysia are not able to differentiate between real news and fake news. Although there are plenty of studies examining fake news, studies examining the mitigation of misinformation through the lens of digital literacy are still rudimentary.

Design/methodology/approach
This research adopted a quantitative approach by conducting a cross-sectional survey among university students in Malaysia to examine how their digital literacy competency influences misinformation. The sample size was estimated GPower software. A total of 134 respondents between the age of 19 and 25 were sampled because young adults in this age group tend to show little difference in their digital literacy level. Structural equation modelling (SEM) was used to examine the proposed model.

Findings
The study results reveal that two of the three domains of digital literacy competence, technical literacy and cognitive literacy, have a positive association in reducing misinformation among university students; however, socio-emotional literacy has the opposite effect. Additionally, the survey also explicates that hedonic motivation helps in misinformation mitigation, whereas habit does not.

Originality/value
Theoretically, this study contributes to the literature by revealing how digital literacy can help in identifying misinformation masquerading as valid information through proper verification and analysis, especially in the digital age where everyone is susceptible to misinformation. The results of the study also contribute to the development of a new digital literacy framework that can cultivate a digitally literate generation who can navigate the informational landscape smartly and therefore distinguish between facts and fake news.
The quality of life in an urban innovation ecosystem: analyzing talents’ perception in Southern Brazil
Authors: Foguesatto Cristian Rogério , Volkmer Martins Bibiana , Tavares da Silveira Fabiane Aparecida , Faccin Kadígia , Balestrin Alsones 
Published: November 2022

Purpose
Talented people with interpersonal skills and competencies are pivotal for creating knowledge, innovation and organizational effectiveness, contributing to local development. In this regard, the quality of life is a critical factor in attracting and retaining talented people in any region. This study aims to analyze talents’ perception of the quality of life in an urban innovation ecosystem. This study considers talents to be the students from Science, Technology, Engineering and Mathematics programs.

Design/methodology/approach
This study analyzes 263 students from three of the country’s most important universities located in the city of Porto Alegre in southern Brazil. This study examines the data using principal component analysis and cluster techniques.

Findings
The results indicate five clusters. The “Love for the city” and the “Mixed” ones portray high levels of a sense of belonging to the city, but differ, for example, in their perception on city infrastructure. Conversely, both the “Worried about education” and the “Worried about commercial services” ones express low levels of a sense of belonging in the city. The “Security” cluster portrays the highest level on security issues in the city. The cluster analyses provide detailed information on the factors valued by talents in urban innovation ecosystems.

Originality/value
To date, to the best of the authors’ knowledge, this study is the first that uses cluster techniques to measure talents’ perception of the quality of life in an urban innovation ecosystem. The findings contribute to mapping talents’ perception and building profiles which may support the development of policies and programs to attract and retain qualified people in innovation ecosystems.
Risk-managed time-series momentum: an emerging economy experience
Authors: Singh Simarjeet , Walia Nidhi , Bekiros Stelios , Gupta Arushi , Kumar Jigyasu , Mishra Amar Kumar 
Published: November 2022

PurposeThis research study aims to design a novel risk-managed time-series momentum approach. The present study also examines the time-series momentum effect in the Indian equity market. Apart from this, the study also proposes a novel risk-managed time-series momentum approach.Design/methodology/approachThe study considers the adjusted monthly closing prices of the stocks listed on the Bombay Stock Exchange from January 1996 to December 2020 to formulate long-short portfolios. Newey–West t statistics were used to test the significance of momentum returns. The present research has considered standard risk factors, i.e. market, size and value, to evaluate the risk-adjusted performance of time-series momentum portfolios.FindingsThe present research reports a substantial absolute momentum effect in the Indian equity market. However, absolute momentum strategies are exposed to occasional severe losses. The proposed time-series momentum approach not only yields 2.5 times higher return than the standard time-series momentum approach but also causes substantial enhancement in downside risks and higher-order moments.Practical implicationsThe study's outcomes offer valuable insights for professional investors, capital market regulators and asset management companies.Originality/valueThis study is one of the pioneers attempting to test the time-series momentum effect in emerging economies. Besides, current research contributes to the escalating literature on risk-managed momentum by suggesting a novel revised time-series momentum approach.
Modeling factors affecting the interests of the container terminal using fuzzy cognitive map and fuzzy DEMATEL
Authors: Khajeh Fatemeh , Shahbandarzadeh Hamid 
Published: November 2022

Purpose
Because container terminals (CTs) are broker organizations, their interest has complex, various internal and external factors, the purpose of this study is to scrutiny and structure the factors affecting the interests of the CT.

Design/methodology/approach
In terms of purpose, this study is a developmental study that, are identified the factors related to the interests of the CT through studying the library and interviewing experts and then the degree of influence of each factor on each other by using the interview with experts of CT in Bushehr province are determined. Then, the fuzzy DEMATEL method is used to calculate the feedback loop of the indicators, and then the matrix obtained Fcmapper software and the fuzzy cognitive map (FCM) is drawn.

Findings
According to the FCM analysis, three important factors are centrality identified in terms of attracting capital, quality of service and efficiency. In addition, there are three factors, attracting capital, operator performance and law have high outdegree.

Originality/value
This research has identified 34 effective indicators of CT interests and evaluated their relationship with FCM. In the available researches, all these indicators measurement has not been evaluated together. Furthermore, fuzzy DEMATEL has been used to evaluate self-loops. Another contribution of this research is the introduction of a strategic route as a roadmap for CT managers in Bushehr province to decide on the interests of a CT.