bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnitude and determinants of intimate partner violence against women in East Africa: multilevel analysis of recent demographic and health survey
Authors: Kebede Sewnet Adem , Weldesenbet Adisu Birhanu , Tusa Biruk Shalmeno 
Published: March 2022

Abstract
Background
Violence against women is a significant public health problem, and human rights abuse, and is associated with multiple adverse physical, mental, sexual, and reproductive health effects. The current study aimed to determine the magnitude of intimate partner violence (IPV) and its determinant factors in East African countries.

Methods
We utilized the most recent demographic and health survey data from 11 East African countries, which was comprised of a weighted sample of 55,501 ever-married women. A multilevel multivariable logistic regression analysis was applied. We used an adjusted odds ratio with a 95% CI and a p value ≤ 0.05 in the multilevel logistic model to declare significant factors associated with IPV.

Results
The overall prevalence of all forms of IPV in East African countries was 32.66% [95% CI 32.27, 33.05], with the highest IPV occurring in Uganda (14.93%) and the lowest IPV recorded in Comoros (0.87%). In the multivariable multilevel logistic regression model, women’s education, residence, sex of household head, current pregnancy, husband drinking alcohol, attitude towards wife-beating husband controlling behavior, and women’s decision-making autonomy were significantly associated with IPV.

Conclusion
The risk factors noted above increase the likelihood of a woman experiencing IPV. Therefore, we recommend establishing effective health and legal response services for IPV, raising awareness of the existing legislation service and improving its application, strengthening legislations on purchasing and selling of alcohol, strengthening joint (both husband and wife) decision-making power by empowering women, improving the educational level of women, and establishing measures to break the culture of societal tolerance towards IPV.
Yoga as an adjunct activity for medical students learning anatomy
Authors: Lee Eugene C. , Adams William , Sandoval-Skeet Noemy , Hoyt Amy , Lee Kit 
Published: March 2022

Abstract
Background
Medical students experience high levels of stress during training due to demanding course loads which often leaves less time for self-care. This study combines the self-care technique of yoga with learning anatomical locations, innervations, actions, and functions of the muscles and organs to determine if anatomy tests scores are improved and whether students’ stress levels attenuate from participating in yoga.

Methods
In this randomized controlled study, 64 student volunteers were randomized into either a yoga intervention group or wait list control group throughout the M1 anatomy course. The yoga group (n = 32) participated in 8 yoga sessions synced with the anatomy topics they were learning in lecture. The wait list group (n = 32) went through their normal anatomy curriculum but had an option to participate in the same yoga sessions after the anatomy course. The primary research purpose was to determine whether yoga improved anatomy exam performance by comparing four anatomy exam scores between the two groups. The secondary research purposes included the following: to determine whether yoga classes including anatomy teaching still conferred acute and long-lasting stress relief by, respectively, comparing a students’ own pre- and post-yoga stress level and self-perceived stress levels between the two groups; and to determine if a student’s confidence in anatomy was improved after a yoga session.

Results
There was no significant difference in anatomy exam performance between students who received yoga and those on the waitlist (all p > 0.05). For students who received yoga, their average self-reported stress levels decreased after each yoga session, their average DASS (Depression, Anxiety and Stress Scale) score decreased after a yoga session, but they were not significantly less stressed than their waitlist peers prior to an exam, and their self-reported confidence in anatomy material related to the back, upper extremity, head and neck, and abdomen/pelvis increased.

Conclusion
With this sample, there was no evidence that yoga sessions paired with anatomy lecture material improved overall anatomy exam performance, as opposed to only the musculoskeletal portion which other studies have looked at. However, yoga acutely reduced stress levels, and subjective feelings of knowledge improvement were noted by participants. Both of these can provide benefits to medical students.
Identification and characterisation of spontaneous mutations causing deafness from a targeted knockout programme
Authors: Lewis Morag A. , Ingham Neil J. , Chen Jing , Pearson Selina , Di Domenico Francesca , Rekhi Sohinder , Allen Rochelle , Drake Matthew , Willaert Annelore , Rook Victoria , Pass Johanna , Keane Thomas , Adams David J. , Tucker Abigail S. , White Jacqueline K. , Steel Karen P. 
Published: March 2022

Abstract
Background
Mice carrying targeted mutations are important for investigating gene function and the role of genes in disease, but off-target mutagenic effects associated with the processes of generating targeted alleles, for instance using Crispr, and culturing embryonic stem cells, offer opportunities for spontaneous mutations to arise. Identifying spontaneous mutations relies on the detection of phenotypes segregating independently of targeted alleles, and having a broad estimate of the level of mutations generated by intensive breeding programmes is difficult given that many phenotypes are easy to miss if not specifically looked for. Here we present data from a large, targeted knockout programme in which mice were analysed through a phenotyping pipeline. Such spontaneous mutations segregating within mutant lines may confound phenotypic analyses, highlighting the importance of record-keeping and maintaining correct pedigrees.

Results
Twenty-five lines out of 1311 displayed different deafness phenotypes that did not segregate with the targeted allele. We observed a variety of phenotypes by Auditory Brainstem Response (ABR) and behavioural assessment and isolated eight lines showing early-onset severe progressive hearing loss, later-onset progressive hearing loss, low frequency hearing loss, or complete deafness, with vestibular dysfunction. The causative mutations identified include deletions, insertions, and point mutations, some of which involve new genes not previously associated with deafness while others are new alleles of genes known to underlie hearing loss. Two of the latter show a phenotype much reduced in severity compared to other mutant alleles of the same gene. We investigated the ES cells from which these lines were derived and determined that only one of the 8 mutations could have arisen in the ES cell, and in that case, only after targeting. Instead, most of the non-segregating mutations appear to have occurred during breeding of mutant mice. In one case, the mutation arose within the wildtype colony used for expanding mutant lines.

Conclusions
Our data show that spontaneous mutations with observable effects on phenotype are a common side effect of intensive breeding programmes, including those underlying targeted mutation programmes. Such spontaneous mutations segregating within mutant lines may confound phenotypic analyses, highlighting the importance of record-keeping and maintaining correct pedigrees.
Acceptance of oclacitinib maleate (Apoquel®) chewable tablets in client-owned dogs with allergic and atopic dermatitis
Authors: Visser Marike , Walsh Kelly , King Vickie , Sture Gordon , Caneva Laura 
Published: March 2022

Abstract
Background
The oral acceptance of oclacitinib maleate (Apoquel®) chewable tablets administered twice daily for 7 days at the labeled dose range of 0.4–0.6 mg/kg was evaluated in 121 dogs treated at ten general practice veterinary clinics in the United States.

Results
Dogs that were enrolled in the study were client-owned, ranged from 1 to 14 years of age, weighed 3.7 to 60.7 kg, and required twice daily treatment with Apoquel for allergic or atopic dermatitis for 7 days. One hundred and twenty-one (121) dogs with 1673 total dose administrations successfully completed the study and were included in the data summary. Out of a total number of 1673 administrations, 1533 (91.6%) were accepted voluntarily within 5 min, 134 (8%) were consumed with assistance (with food treats or by pilling) outside of the 5 min offering time and 6 (0.4%) doses were not consumed. The per dose percent acceptance rate for the 14 offered doses showed minimal variation ranging from 89.9 to 93.3%.

Conclusions
Client-owned dogs from the general veterinary patient population that required treatment with oclacitinib found the chewable tablets to be very palatable and no aversion occurred with repeated dosing. Oclacitinib chewable tablets were well tolerated and are a palatable alternative to the film-coated tablet.
Molecular phylogeny of Anopheles nivipes based on mtDNA-COII and mosquito diversity in Cambodia-Laos border
Authors: Zhang Yilong , Zhang Canglin , Yang Rui , Luo Chunhai , Deng Yan , Liu Yan , Zhang Yilong , Zhou Hongning , Zhang Dongmei 
Published: March 2022

Abstract
Background
Few studies have been conducted to investigate the distribution of mosquito vectors and the population structure of secondary vectors in the border region of Cambodia-Laos. The aim of this work was to study the mosquito diversity and molecular phylogeny of secondary vectors, i.e., Anopheles nivipes in this area.

Methods
1440 adult mosquitoes were trapped in the Cambodia-Laos border. mtDNA-COII were amplified and sequenced from 53 An. nivipes DNA samples. Together with COII sequences deposited in GenBank, a total of 86 COII sequences were used for examining population variations, genetic differentiation, spatial population structure, population expansion, and gene flow patterns.

Results
The adult mosquitoes were classified into 5 genera and 27 species in this border region. The predominant genera were Culex (60.07%, 865/1440) and Anopheles (31.25%, 450/1440), and the major Anopheles species were An. nivipes (73.56%, 331/450) and Anopheles maculatus (14.22%, 64/450). Based on sequences analysis of COII, a high level of genetic differentiation was reported in two Northwest India (Cheema and Bathinda, Punjab) and Cambodia-Laos (Siem Pang, Stung treng) populations (FST = 0.97824, 0.97343, P < 0.05), as well as lower gene flow (Nm = 0.01112, 0.01365) in the An. nivipes populations. Phylogenetic analysis and SAMOVA revealed a gene barrier restricting gene flow among three An. nivipes populations. Mantel test suggested a significant correlation between geography and gene distance in all An. nivipes populations (Z = 44,983.1865, r = 0.5575, P = 0.0070). Neutrality test and Mismatch distribution revealed a recent population expansion of An. nivipes in the Cambodia-Laos population.

Conclusions
Anopheles nivipes was one of the major Anopheles species in the Cambodia-Laos border. Based on sequences analysis of COII, a genetic barrier between Cambodia-Laos and two Indian populations was found, and a recent population expanding or selecting of An. nivipes occurred in the Cambodia-Laos population, suggesting that COII might be an effective marker for describing the molecular phylogeny of An. nivipes. Further investigation and continuous surveillance of An. nivipes are warranted in this region.
Cleavage-stage human embryo arrest, is it embryo genetic composition or others?
Authors: Orvieto Raoul , Jonish-Grossman Anat , Maydan Sharon Avhar , Noach-Hirsh Meirav , Dratviman-Storobinsky Olga , Aizer Adva 
Published: March 2022

SummaryEmbryo transfer is a crucial step in IVF cycle, with increasing trend during the last decade of transferring a single embryo, preferably at the blastocyst stage. Despite increasing evidence supporting Day 5 blastocyst-stage transfer, the optimal day of embryo transfer remains controversial. The crucial questions are therefore, whether the mechanisms responsible to embryos arrest are embryo aneuploidy or others, and whether those embryos arrested in-vitro between the cleavage to the blastocyst stage would survive in-vivo if transferred on the cleavage-stage. We therefore aim to explore whether aneuploidy can directly contribute to embryo development to the blastocyst stage. Thirty Day-5 embryos, that their Day-3 blastomere biopsy revealed a single-gene defect, were donated by 10 couples undergoing preimplantation genetic testing treatment at our center. Affected high quality Day-3 embryos were cultured to Day-5, and were classified to those that developed to the blastocyst-stage and those that were arrested. Each embryo underwent whole genome amplification. Eighteen (60%) embryos were arrested, did not develop to the blastocyst stage and 12 (40%) have developed to the blastocyst stage. Nineteen embryos (63.3%) were found to be euploid. Of them, 12 (66.6%) were arrested embryos and 7 (58.3%) were those that developed to the blastocyst-stage. These figures were not statistically different (p = 0.644). Our observation demonstrated that the mechanism responsible to embryos arrest in vitro is not embryo aneuploidy, but rather other, such as culture conditions. If further studies will confirm that Day-5 blastocyst transfer might cause losses of embryos that would have been survived in vivo, cleavage-stage embryo transfer would be the preferred timing. This might reduce the cycle cancellations due to failure of embryo to develop to the blastocyst stage and will provide the best cumulative live birth-rate per started cycle.
Effect of combined wet alkaline mechanical pretreatment on enzymatic hydrolysis of corn stover and its mechanism
Authors: Yang Jie , Gao Chongfeng , Yang Xueqi , Su Yanfu , Shi Suan , Han Lujia 
Published: March 2022

Abstract
Background
To further optimize the mechanochemical pretreatment process, a combined wet alkaline mechanical pretreatment of corn stover was proposed with a short time and less chemical consumption at room temperature.

Results
The combined alkaline mechanical pretreatment significantly enhanced enzymatic hydrolysis resulting a highest glucose yield (YG) of 91.9% with 3% NaOH and ball milling (BM) for 10 min. At this optimal condition, 44.4% lignin was removed and major portion of cellulose was retained (86.6%). The prehydrolysate contained by-products such as monosaccharides, oligosaccharides, acetic acid, and lignin but no furfural and 5-HMF. The alkaline concentration showed a significant impact on glucose yield, while the BM time was less important. Quantitative correlation analysis showed that YG (%) = 0.68 × BM time (min) + 19.27 × NaOH concentration (%) + 13.71 (R2 = 0.85), YG = 6.35 × glucan content − 231.84 (R2 = 0.84), and YG =  − 14.22 × lignin content + 282.70 (R2 = 0.87).

Conclusion
The combined wet alkaline mechanical pretreatment at room temperature had a boosting effect on the yield of enzymatic hydrolysis with short treatment time and less chemical consumption. The impact of the physical and chemical properties of corn stover pretreated with different BM times and/or different NaOH concentrations on the subsequent enzymatic hydrolysis was investigated, which would be beneficial to illustrate the effective mechanism of the mechanochemical pretreatment method.
Multi-omic alterations of the SWI/SNF complex define a clinical subgroup in lung adenocarcinoma
Authors: Peinado Paola , Andrades Alvaro , Cuadros Marta , Rodriguez Maria Isabel , Coira Isabel F. , Garcia Daniel J. , Benitez-Cantos Maria S. , Cano Carlos , Zarzuela Eduardo , Muñoz Javier , Loidi Claudia , Saiz Monica , Medina Pedro P. 
Published: March 2022

AbstractSWI/SNF complexes are major targets of mutations in cancer. Here, we combined multiple “-omics” methods to assess SWI/SNF composition and aberrations in LUAD. Mutations in lung SWI/SNF subunits were highly recurrent in our LUAD cohort (41.4%), and over 70% of the mutations were predicted to have functional impact. Furthermore, SWI/SNF expression in LUAD suffered an overall repression that could not be explained exclusively by genetic alterations. Finally, SWI/SNF mutations were associated with poorer overall survival in TCGA-LUAD. We propose SWI/SNF-mutant LUAD as a separate clinical subgroup with practical implications.
Racism in Australia: a protocol for a systematic review and meta-analysis
Authors: Ben Jehonathan , Elias Amanuel , Issaka Ayuba , Truong Mandy , Dunn Kevin , Sharples Rachel , McGarty Craig , Walton Jessica , Mansouri Fethi , Denson Nida , Paradies Yin 
Published: March 2022

AbstractBackgroundRacism has been identified as a major source of injustice and a health burden in Australia and across the world. Despite the surge in Australian quantitative research on the topic, and the increasing recognition of the prevalence and impact of racism in Australian society, the collective evidence base has yet to be comprehensively reviewed or meta-analysed. This protocol describes the first systematic review and meta-analysis of racism in Australia at the national level, focussing on quantitative studies. The current study will considerably improve our understanding of racism, including its manifestations and fluctuation over time, variation across settings and between groups, and associations with health and socio-economic outcomes.MethodsThe research will consist of a systematic literature review and meta-analysis. Searches for relevant studies will focus on the social and health science databases CINAHL, PsycINFO, PubMed and Scopus. Two reviewers will independently screen eligible papers for inclusion and extract data from included studies. Studies will be included in the review and meta-analysis where they meet the following criteria: (1) report quantitative empirical research on self-reported racism in Australia, (2) report data on the prevalence of racism, or its association with health (e.g. mental health, physical health, health behaviours) or socio-economic outcomes (e.g. education, employment, income), and (3) report Australian data. Measures of racism will focus on study participants’ self-reports, with a separate analysis dedicated to researcher-reported measures, such as segregation and differential outcomes across racial/ethnic groups. Measures of health and socio-economic outcomes will include both self-reports and researcher-reported measures, such as physiological measurements. Existing reviews will be manually searched for additional studies. Study characteristics will be summarised, and a meta-analysis of the prevalence of racism and its associations will be conducted using random effects models and mean weighted effect sizes. Moderation and subgroup analyses will be conducted as well. All analyses will use the software CMA 3.0.DiscussionThis study will provide a novel and comprehensive synthesis of the quantitative evidence base on racism in Australia. It will answer questions about the fluctuation of racism over time, its variation across settings and groups, and its relationship with health and socio-economic outcomes. Findings will be discussed in relation to broader debates in this growing field of research and will be widely disseminated to inform anti-racism research, action and policy nationally.Systematic review registrationPROSPEROCRD42021265115.
Truths of the Riverscape: Moving beyond command-and-control to geomorphologically informed nature-based river management
Authors: Brierley Gary , Fryirs Kirstie 
Published: March 2022

AbstractTruths of the Riverscape refer to the use of geomorphological principles to inform sustainable approaches to nature-based river management. Across much of the world a command-and-control philosophy continues to assert human authority over rivers. Tasked to treat rivers as stable and predictable entities, engineers have ‘fixed rivers in place’ and ‘locked them in time’. Unsustainable outcomes ensue. Legacy effects and path dependencies of silenced and strangled (zombified) rivers are difficult and increasingly expensive to address. Nature fights back, and eventually it wins, with disastrous consequences for the environment, society, culture and the economy. The failure to meet the transformative potential of nature-based applications is expressed here as a disregard for ‘Truths of the Riverscape’. The first truth emphasises the imperative torespect diversity, protecting and/or enhancing the distinctive values and attributes of each and every river. A cross-scalar (nested hierarchical) lens underpins practices that ‘know your catchment’. The second truth envisages management practices thatwork with processes, interpreting the behaviour of each river. This recognises that erosion and deposition are intrinsic functions of a healthy living river—in appropriate places, at appropriate rates. This premise underpins the third truth,assess river condition, highlighting the importance of what to measure and what to measure against in approaches that address the causes rather than the symptoms of unexpected river adjustment. The fourth truthinterprets evolutionary trajectory to determine what is realistically achievablein the management of a given river system. Analysis of whether the river sits on a degradation or recovery pathway (i.e., condition is deteriorating or improving), alongside assessment of catchment-specific recovery potential, is used to foresight river futures. Viewed collectively, Truths of the Riverscape provide a coherent platform to develop and apply proactive and precautionary catchment management plans that address concerns for biodiversity loss and climate change adaptation.
STEM courses are harder: evaluating inter-course grading disparities with a calibrated GPA model
Authors: Tomkin Jonathan H. , West Matthew 
Published: March 2022

AbstractBackgroundGrades in college and university STEM courses are an important determinant of student persistence in STEM fields. Recent studies have used the grade offset/grade penalty method to explore why students have lower grades in STEM courses than their GPAs would predict. The results of these studies are in doubt; however, as they use GPA as a reliable measure of academic performance, which is a disputed assumption. Using a predictive model of student performance, it is possible to produce a more accurate measure of academic performance than the observed GPA and discover if STEM courses are graded more stringently, and under which circumstances.ResultsA weighted logistic model of GPA better predicts academic performance than the observed GPA. Using this calibrated GPA it is found that the grade offset method predicts that STEM courses, departments, and programs grade significantly more stringently than non-STEM courses. The average grade difference between STEM and non-STEM course grades and GPAs is around four tenths of a grade point. An exception is general education courses offered by STEM departments, which are graded with the same leniency as non-STEM courses. Grade offset calculations that use the observed GPA systematically underestimate the negative offset in STEM grading relative to calculations that use the calibrated GPA. The calibrated GPA is much more highly correlated with standardized tests such as the ACT (r = 0.49) than the observed GPA is (r = 0.25).ConclusionObserved GPA is a systematically biased measure of academic performance, and should not be used as a basis for determining the presence of grading inequity. Logistic models of GPA provide a more reliable measure of academic performance. When comparing otherwise academically similar students, we find that STEM students have substantially lower grades and GPAs, and that this is the consequence of harder (more stringent) grading in STEM courses.