bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using a Community of Transformation to create a sustained change toward increased active learning in the undergraduate classroom
Authors: Prunuske Amy J. , Evans-Anderson Heather J. , Furniss Katherine L. , Goller Carlos C. , Mirowsky Jaime E. , Moore Michael E. , Raut Samiksha A. , Swamy Uma , Wick Sue , Wolyniak Michael J. 
Published: September 2022

Abstract
Background: Many individuals and groups have responded to the call to action to reform undergraduate biology teaching through increased utilization of evidence-based teaching strategies. One promising practice for creating sustained change is the use of a Community of Transformation or a deliberate network designed to reinforce active learning through mentorship. The Promoting Active Learning and Mentoring (PALM) Network, which encourage its members to reflect deeply on their teaching and to make changes based on evidence-based practices, supports the implementation of active learning through a network of practitioners from across multiple professional societies and disciplines. Members of the Network interviewed seven previous PALM Fellows, one to four years after completing their fellowship, to better understand the users of the network and why they engaged with the network.
Results: Key themes emerged from interviews about how engagement and experiences of each of the interviewees aligned with the ADKAR model of change management: Awareness, Desire, Knowledge, Ability, and Reinforcement. The interviews supported the creation of three personas: Bob the Sponge, Lupe the Lone Wolf, and Malik the Master. The interviews also highlighted examples of how the Fellows continue to utilize evidence-based teaching practices and how they successfully implemented adaptation to online course interactions during the COVID-19 pandemic.
Conclusions, summary, and implications: Understanding how the personas intersect with the ADKAR model has led to a better understanding of how the PALM network functions as a community of transformation to facilitate transformative change toward active learning. This approach can serve as a model for others who wish to enable similar transformative change in various Science, Technology, Engineering, and Math (STEM) disciplines.
Socio-economic factors associated with substance use across different waves of the COVID-19 pandemic: an intersectional analysis of a repeated cross-sectional survey
Authors: Somé Nibene H. , Imtiaz Sameer , Wells Samantha , de Oliveira Claire , Hamilton Hayley A. , Ali Shehzad , Elton-Marshall Tara , Rehm Jürgen , Shield Kevin D. 
Published: September 2022

Abstract

Background
This study examined trends in cannabis and alcohol use among Canadian adults and across socio-economic subgroups over four waves of the COVID-19 pandemic from 2020 to 2022. Interactions between socio-economic status (SES) and gender, ethnicity/race, and age as they are associated with alcohol and cannabis use were examined.
Methods
Data were obtained from nine successive web-based cross-sectional surveys of adults living in Canada (8,943 participants) performed from May 2020 to January 2022. Substance use measurements included self-reported changes in alcohol and cannabis use compared to before the pandemic, heavy episodic drinking (HED) (i.e., consumption of 4 or 5 standard drinks on one drinking occasion in the past 7 days for men and women respectively), and cannabis use in the past 7 days. The Wilcoxon rank-sum test was used to test for equality of the prevalence of substance use. Logistic regression models were used to assess the associations of SES and its interactions with gender, ethnicity/race, and age with alcohol and cannabis use.
Results
The prevalence of increased alcohol and cannabis use differed through the pandemic waves depending on SES. The prevalence of HED and increased cannabis use were similar across SES groups. Having a moderate or high household income, and being unemployed was associated with HED and increased alcohol use. Women with a high household income ($120,000+) and a high household income and aged 40–59 years were associated with increased alcohol use compared to men. Non-White individuals who reported other impacts of COVID-19 on their work had lower odds of reporting increased alcohol use. Protective factors associated with cannabis use included having a university degree, being a senior with a moderate/high household income, being aged 40–59 years with a university degree, being a senior with at least a post-secondary level education, and being a highly educated non-White individual.
Conclusions
Associations between SES and substance use differ by gender, race and age. To reduce health disparities, public health interventions should account for these interactions.
Fibrinogen promotes gallbladder cancer cell metastasis and extravasation by inducing ICAM1 expression
Authors: Jiang Chengkai , Li Yang , Li Yongsheng , Liu Liguo , Wang Xu-an , Wu Wenguang , Bao Runfa , Weng Hao , Li Maolan , Shu Yijun , Liu Yingbin 
Published: September 2022

Abstract
Fibrinogen (FIB) plays an important role in tumor progression. Here, we explored role of fibrinogen in gallbladder cancer (GBC) metastasis. The plasma fibrinogen level in M1 GBC patients was higher than in M0 GBC patients, indicating that fibrinogen may participate in GBC metastasis. Treatment of GBC cell lines with fibrinogen promoted metastasis and induced the expression of intercellular adhesion molecule 1 (ICAM1). ICAM1 overexpression promoted metastasis and knockdown inhibited it. The cell adhesion and transendothelial migration of GBC cells were enhanced by fibrinogen treatment and ICAM1 overexpression. In addition, the medium of fibrinogen-treated and overexpression-ICAM1 NOZ cells exhibited enhanced macrophages recruitment. This may work in concert to promote angiogenesis. Immunohistochemistry results on clinical specimens showed that higher fibrinogen levels, higher ICAM1 expression, higher blood vessel density, and higher macrophage levels were present simultaneously. Collectively, this study indicates fibrinogen promotes metastasis and extravasation by inducing ICAM1 expression to enhance tumor cell migration, cell adhesion, transendothelial migration and promote angiogenesis and increase vascular endothelial permeability.
Comparison of the macular choroidal and microvascular network changes in thyroid eye patients with different activity scores and the effect of serum antibodies
Authors: Dogan Mehmet Erkan , İlhan Deniz , ayaz yusuf 
Published: September 2022

Abstract
Objective
To investigate the choroidal thickness and the microvascular network changes around the macula in thyroid eye disease (TED) patients at different stages and the relationship of those changes with risk factors, serum antibodies and the severity of TED
Material and Methods
A total of 85 participants were enrolled. All participants underwent ophthalmology and endocrinology examinations. Central choroidal thickness (CCT), superficial (s) and deep (d) foveal avascular zone (FAZ) area, mean (m) and central (c) macular vascular density (SVD, DVD) measurements of the enrolled cases were performed with Topcon Swept Source OCT / OCT-Angiography DRI OCT Triton (Topcon Co. Japan). Multiple linear regression analysis was used to explore the associations between CCT, FAZ area, SVD, DVD and the relevant factors of TED.
Results
It was found that those with active TED patients had higher c-DVD and m-DVD levels (p < 0.05), however there is no statistically significant difference in CCT between active and stable TED patients. Among the serum antibodies, it was observed that the CCT thickened only in patients with high thyroid stimulating hormone-receptor autoantibodies (TRAB) and human thyroglobulin (HTG) level also s-FAZ and d-FAZ increased, c-SVD and m-SVD decreased in patients with high TRAB level. There was no significant difference in CCT, FAZ, SVD and DVD measurement at gender, between hyperthyroid and euthyroid patients and among those with or without thyroid papillary ca.
Conclusions
The results showed that both disease activation and serum antibodies differentially affect both superficial and deep retinal vascular density. It has also been shown that high serum antibody levels affect choroidal thickness independent of clinical activity.
Efficacy of Lycium Barbarum Polysaccharide on Cytokine Response in Youths with Subthreshold Depression
Authors: Li Xiaoyue , Liu Tao , Mo Xuan , Wang Runhua , Kong Xueyan , Shao Robin , McIntyre Roger S. , So Kwok-Fai , Lin Kangguang 
Published: September 2022

Abstract
Background: Elevated levels of inflammatory cytokines such as Interleukin (IL)-17A have been repeatedly linked with major depression in both animals and humans. Our recent double-blinded Randomized Placebo-controlled Trial (RCT) discovered significant efficacy of a traditional Chinese medicine substance, namely the Lycium barbarum polysaccharide (LBP), on reducing depressive symptoms among adolescents with subthreshold depression. Here, we further investigated whether the antidepressant property of LBP was associated with its anti-inflammatory effect on adolescents with subthreshold depression.
Methods: In this 6-week RCT, we measured common cytokine levels from participants’ peripheral blood samples, both at baseline and at the end of the 6-week intervention with either LBP (intervention group, N=14) or placebo (control group, N=10). Independent t-tests were used to compare the change of cytokine levels between groups.Network-based analysis was applied to evaluate the systemic immune responses to the interventions.
Results: The intervention and control groups were matched on demographic and clinical characteristics. As expected, the LBP group exhibited a greater reduction in IL-17A compared to the placebo group (t = -2.25, P = 0.04) after the 6-week interventions. Moreover, network-level analysis showed that LBP reduced the correlations and connectivity among cytokines (Z = 3.34, P < 0.01), which was in turn associated with improvement of depressive symptoms.
Conclusions: Our findings demonstrated that 6-week repeated LBP administrations downregulated immune responses in adolescents with StD, which could be a key mechanistic pathway underpinning the antidepressant effect of LBP.
Application of modified peck formula in surface settlement of hejiapo tunnel
Authors: Yan Sheng-Hui , Wen Chang-Ping 
Published: September 2022

Abstract
In order to accurately predict the surface settlement caused by the excavation of shallow buried tunnels in mountain areas, timely control the future settlement of the surface of the non excavated section, and ensure the absolute safety in the construction process, this paper uses peck formula to predict the surface settlement of hejiapo tunnel of Guiyang Jinsha expressway. Because there is a large error between the predicted data of Peck and the measured data, this paper intends to use the linear regression fitting method in quantitative statistics to fit and correct the measured surface settlement data, and introduce the maximum settlement correction coefficient and the settlement tank width correction coefficient. Through the analysis of a large number of cross-sectional measured data, the empirical range of the correction coefficient of the local peck formula is obtained, and the peck formula is corrected. The results show that the modified peck formula curve is highly linearly related to the actual surface settlement curve, and the accuracy of the modified peck formula is the highest when the correction coefficient α∈ (0.6, 1.2) and β∈ (0.5, 0.8). A section is randomly selected for verification. The results show that the predicted value after section excavation is highly linearly related to the measured value. From the research results, it can be seen that peck formula can predict the surface settlement caused by the construction of shallow buried tunnel by the underground excavation method, but there is a large error with the actual settlement curve. It is necessary to refer to the measured data to fit and correct peck formula. The modified peck formula plays a practical guiding role in the subsequent construction of Hejia broken tunnel.
Molluscs organisms diversity and temperature fluctuation of Cap Estérias rocky foreshore (Gabon)
Authors: Zongo Sylvie Brizard , Ngohang Franck Estimé , Mabert Brice Didier Celce Koumba , Moundounga Nick Roland 
Published: September 2022

Abstract
This study has made it possible to link the configuration and distribution of molluscs in the Cape Estérias mediolittoral rocky foreshore area, from January 2021 to January 2022, during a one-year cycle. In fact, the intertidal area being a fragile and above all unstable, it necessitates regular and frequent monitoring. The malacofauna were then taken every two weeks during spring tides. And the temperature was measured twice a day in situ during the two tides. The Cap Estérias rocky foreshore malacofauna reveals 36 molluscs belonging to 3 families (29 gastropods, 6 bivalves and 1 cephalopod). As for the water temperature, it shows large fluctuations with average amplitude of 0.75 °C, probably due to tidal dynamics and the Mondah fresh water in flows. These temperatures vary from 32.2 to 23.5 °C, for an average of around 28.5 ± 1.5 °C year - round. To end, the highest water temperatures are recorded during the rainy seasons, while the lowest are recorded during the dry seasons; this is in accordance with the equatorial climate of the studied area.
The evaluation of both the expression and serum protein levels of Caspase-8 and Mitogen-activated protein kinases one genes in patients with different degrees of SARS-CoV-2 infection
Authors: acat murat , gülhan pınar yıldız , Eroz Recep , Özkan Ayşegül Ertınmaz , Koca Oğuzhan , Çınar Caner 
Published: September 2022

Abstract
Aim:We performed the current study to evaluate the effects of caspase-8 (CASP8), Mitogen-activated protein kinase 1 (MAPK1) gene expression levels, and their products on preventing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.
Methods:40 individuals with SARS-CoV-2 infection were included in the current study. The patients were divided into four main groups mild (n=7), moderate (n=10), severe(n=14), and critical(n=9) levels of the disease. Individuals under the age of 18 and pregnant women were excluded. Patients were classified according to the WHO classification system (WHO/2019-nCoV/clinical/2021.1).
Results: Totally 40 individuals (male: 15 (37.5%); female: 25 (62.5%) with SARS-CoV2 infection were included in the current study. When all groups were taken into consideration, statistically significant differences were detected among all groups for both CASP82-ΔΔCt(p=0.006) and MAPK1 2-ΔΔCtvalues (p=0.015). Also, statistically significant differences were detected between mild and moderate (p=0.013), moderate and critical (p=0.018), and severe and critical (p=0.023) groups for lymphocytes.
Conclusions:The CASP8/MAPK1 expression levels and/or its products are essential in preventing injury caused by SARS-CoV-2 infection. They play crucial roles in maintaining cellular homeostasis and viability. Perhaps CASP8/MAPK1 levels can provide information about the severity of the disease.
Effect of Tenofovir on gut microbiota and inflammatory factors in HBV-infected individuals
Authors: Jianfei Long , Pan Zhang , Yu Zhang , Ling Li , Shuangmei Tong , Jingru Gong , Han Zhu , Xiaolin Liu , Hongyan Ren , Chao Liu , Jiming Zhang , Bin Wang 
Published: September 2022

Abstract
Background
Studies have found dysbiosis of the gut microbiota in hepatitis B virus (HBV)-infected individuals. Tenofovir dipivoxil (TDF) is one of the preferred oral antiviral drugs for the treatment of chronic hepatitis B (CHB), but the effect of TDF on gut microbiota and inflammatory factors remains unexplored.
Methods
In this study, we prospectively collected stool samples from unmedicated HBV patients and CHB patients treated with TDF. Gut microbiota and inflammatory factors were studied in 42 healthy subjects (HC group), 109 HBV-infected individuals, including 48 CHB patients who did not take nucleoside analogue drugs (No-NAs group) and 61 CHB patients who took TDF (TDF group).
Results
16S rRNA sequencing revealed that TDF treatment caused significant alterations in intestinal microbiota in HBV-infected individuals, however, HBV-infected individuals could not fully recover from intestinal microbiota dysbiosis. The relative abundance of Bacteroidota decreased gradually from HC group to No-NAs and TDF groups. Firmicutes and Actinobacteria were more abundant in No-NAs and TDF groups than in the HC group. The relative abundance of Fusobacteriota was significantly higher in the No-NAs group than those in HC group. At the genus level, Dialister, Eubacterium_hallii_group, Halomonas, Collinsella, Sphingomonas, Xanthomonadaceae_unclassified, and Rhizobiaceae_unclassified were found to be overrepresented, while the abundances of Bacteroides and Fusobacterium were significantly decreased in No-NAs and TDF groups.
Conclusions
This study showed that TDF treatment significantly improved gut microbiota dysregulation. In addition, we did not observe a significant improvement in serum inflammatory factor levels, which may be related to the relatively short duration of TDF administration in this study.
Factors affecting insulation failure in reusable surgical devices
Authors: Homma Takahiro 
Published: September 2022

Abstract
Background and Aims: The purpose of this study was to investigate the associated factors of IF in reusable endoscopic instruments.
Methods: The insulation coating of reusable endoscopic instruments underwent routine visual checks, hand washing to remove visible stains, and mechanized sterilization. We recorded the cleaning number and usage period of all instruments. The instruments were tested for IF using a detector.
Results: IF was found in eight of 69 devices (11.6%). Examining by clinical specialty, we found IF in 4 of 28 gastrointestinal (14.3%), 3 of 20 gynecological (15.0%), 1 of 12 urological (8.3%), and none of the nine thoracic devices. The median distance from the tip to the damaged part was 5 cm (3–5 cm). In the IF and the intact groups, the period of use (7 years [6–8] versus 7 years [4–8], P = 0.90) and the number of cleanings (281 [261–323] versus 261 [179–320], P = 0.27) were not significantly different. The IF group included products of three different companies; however, six of the eight (75.0%) were from the same company.
Conclusions: Cleaning methods and usage period might have a smaller effect on IF than the instrument insulation and usage methods.
Robust Calibration Estimation of Population Mean in Stratified Sampling in the Presence of Outlier
Authors: Babatunde Oluwagbenga T. , Oladugba Abimibola V. 
Published: September 2022

Abstract
A new improved calibration estimator for the population mean in a stratified sampling in the presence of an outlier in the auxiliary variable was proposed in this paper. The median of the auxiliary variable was used in defining the calibration constraints. Simulation study as well as empirical study were performed to establish the performance of the proposed estimator over some existing estimators. The results of both simulation and empirical studies show that the proposed calibration estimator performed better and more efficient to all the existing calibration estimators in this work.
Photobiomodulation upregulates neuroligin-3 and improves the synapses and cognitive function and ameliorates epileptic seizure
Authors: Hong Namgue , Kim Hee Jung , Kang Keunsoo , Kim Hyung-Gun , Kang Bong Hui , Chung Phil-Sang , Lee Min Young , Ahn Jin-Chul 
Published: September 2022

Abstract
Background
Temporal lobe epilepsy (TLE) remains one of the most drug-resistant focal epilepsies. Glutamate excitotoxicity and neuroinflammation which leads to loss of synaptic proteins and neuronal death appear to represent a pathogen that characterizes the neurobiology of TLE. Photobiomodulation (PBM) is a rapidly growing therapy for the attenuation of neuronal degeneration harboring non-invasiveness benefits. However, the detailed effects of PBM on excitotoxicity or neuroinflammation remain unclear. We investigated whether tPBM exerts neuroprotective effects on hippocampal neurons in epilepsy mouse model by regulating synapse and synapse-related genes.
Methods
In an in vitro study, we performed imaging analysis and western blot in primary hippocampal neurons from embryonic (E17) rat pups. In an in vivo study, histological stain and immunohistochemistry analyses were used to assess neuronal survival, synaptic connections and neuroinflammation. Behavioral tests were used to evaluate the effects of PBM on cognitive functions. RNA sequencing was performed to identify the gene regulatory by PBM.
Results
PBM was upregulated synaptic connections in an in vitro. In addition, it was confirmed that transcranial PBM reduced neuronal apoptosis, synaptic degeneration, and neuroinflammation in an in vivo. These effects of PBM were supported by RNA sequencing results showing the relation of PBM with gene regulatory networks of neuronal functions. Specifically, Nlgn3 showed robust increase after PBM and silencing the Nlgn3 reversed the positive effect of PBM in in vitro. Lastly, behavioral alterations including hypoactivity, anxiety and impaired memory were recovered along with the reduction of seizure score in PBM-treated mice.
Conclusions
Our findings demonstrate that PBM attenuates epileptic excitotoxicity, neurodegeneration and cognitive decline induced by TLE through gene regulation of the neuronal developments including Nlgn3.
Expression of the angiogenesis marker endocan in Cushing's syndrome due to cortisol producing adenoma
Authors: Can Mustafa , Kocabaş Muhammet , Karaköse Melia , Esen Hacı Hasan , Kulaksızoğlu Mustafa , Karakurt Feridun 
Published: September 2022

Abstract
Purpose: In this study, we aimed to investigate the endocan expression in tissue samples of patients diagnosed with Cushing's syndrome (CS) due to Cortisol-Producing-Adenoma (CPA) and compare it with normal adrenal cortex tissue, and also to evaluate the correlations of endocan expression with clinical and histopathological features.
Methods: 11 patients who were operated for CS due to CPA between 2009-2021 and 14 control subjects with normal adrenal cortex were included in the study. Demographic, laboratory and clinicopathological data of the patients were recorded. Sections of 4-5 µm thickness were taken from paraffin blocks of patients diagnosed with CS due to CPA and control subjects with normal adrenal cortex tissue. The sections were then stained in a closed system automatic immunohistochemical staining device to perform immunohistochemical analysis. The endocan positive grade were determined based on the proportion of stained cells on a scale of negative to strong.
Results: The number of subjects with positive endocan expression and the mean endocan expression level in the CS group were significantly higher than the control group (p=0.005, p<0.001, respectively). No correlation was found between endocan expression and clinical and histopathological features
Conclusion: According to the results of our study, endocan overexpression in CPA tissues may be related to the hormonal functionality of CPA.