bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in alcohol use during hepatitis C treatment in persons who inject drugs
Authors: Martin Madhuri , Roth Prerana J. , Niu Jiajing , Pericot‐Valverde Irene , Heo Moonseong , Padi Akhila , Norton Brianna L. , Akiyama Matthew J. , Litwin Alain H. 
Published: August 2022

AbstractPeople who inject drugs (PWID) are a vulnerable population at high risk for acquiring hepatitis C virus (HCV) and frequently suffer from comorbid alcohol use. This study examines the characteristics and correlates of alcohol use among study participants, the association between alcohol consumption and sustained virologic response (SVR) in patients receiving HCV treatment, changes in drinking behaviours during HCV treatment and associations of drinking over time with specific models of HCV treatment. Participants were 150 PWID with HCV who were receiving opioid agonist therapy (OAT) and enrolled in a randomized clinical trial exploring the effectiveness of three models of care for HCV treatment. The addiction severity index was the primary measure of alcohol consumption. Days of alcohol intake were evaluated longitudinally and across three treatment groups. At baseline, 31% (47/150) reported having at least one drink in the last 30 days including 24% (36/150) who reported drinking to intoxication in the last 30 days. There was no difference in SVR rates between groups. There was a significant decrease in overall days of drinking from baseline (7.78 ± 7.86) to follow‐up at Week 24 (5.78 ± 8.83) (p = 0.041), but there were no significant changes among those who drank to intoxication; modified directly observed therapy (mDOT) was the only group with a significant decline in days of alcohol consumption (p = 0.041). In this cohort of PWID on OAT, baseline alcohol consumption did not affect SVR rates. HCV treatment was overall associated with decreased alcohol consumption. In particular, mDOT was associated with decreased alcohol consumption. Given the additive effect of alcohol and HCV on the development of cirrhosis, studies should be done to investigate the complimentary effects of the mDOT model of care on alcohol cessation.
Genome‐wide DNA methylation patterns harbour signatures of hatchling sex and past incubation temperature in a species with environmental sex determination
Authors: Bock Samantha L. , Smaga Christopher R. , McCoy Jessica A. , Parrott Benjamin B. 
Published: August 2022

AbstractConservation of thermally sensitive species depends on monitoring organismal and population‐level responses to environmental change in real time. Epigenetic processes are increasingly recognized as key integrators of environmental conditions into developmentally plastic responses, and attendant epigenomic data sets hold potential for revealing cryptic phenotypes relevant to conservation efforts. Here, we demonstrate the utility of genome‐wide DNA methylation (DNAm) patterns in the face of climate change for a group of especially vulnerable species, those with temperature‐dependent sex determination (TSD). Due to their reliance on thermal cues during development to determine sexual fate, contemporary shifts in temperature are predicted to skew offspring sex ratios and ultimately destabilize sensitive populations. Using reduced‐representation bisulphite sequencing, we profiled the DNA methylome in blood cells of hatchling American alligators (Alligator mississippiensis), a TSD species lacking reliable markers of sexual dimorphism in early life stages. We identified 120 sex‐associated differentially methylated cytosines (DMCs; FDR < 0.1) in hatchlings incubated under a range of temperatures, as well as 707 unique temperature‐associated DMCs. We further developed DNAm‐based models capable of predicting hatchling sex with 100% accuracy (in 20 training samples and four test samples) and past incubation temperature with a mean absolute error of 1.2°C (in four test samples) based on the methylation status of 20 and 24 loci, respectively. Though largely independent of epigenomic patterning occurring in the embryonic gonad during TSD, DNAm patterns in blood cells may serve as nonlethal markers of hatchling sex and past incubation conditions in conservation applications. These findings also raise intriguing questions regarding tissue‐specific epigenomic patterning in the context of developmental plasticity.
Burnout, wellbeing and how they relate: A qualitative study in general practice trainees
Authors: Prentice Shaun , Elliott Taryn , Dorstyn Diana , Benson Jill 
Published: August 2022

AbstractPurposeAlthough research has explored burnout risk factors among medical trainees, there has been little exploration of the personal experiences and perceptions of this phenomenon. Similarly, there has been little theoretical consideration of trainee wellbeing and how this relates to burnout. Our study aimed to conceptualise both constructs.MethodWe situated this study within a post‐positivist epistemology using grounded theory to guide the research process. Participants were recruited from one Australian General Practice training organisation. Fourteen trainees completed interviews, while a further five focus groups explored the views of 33 supervisors, educators and training coordinators. Data collection and analysis occurred concurrently, drawing upon constant comparison and triangulation. Template analysis, using an iterative process of coding, was employed to generate conceptual models of the phenomena of interest.ResultsParticipants described burnout as an insidious syndrome lying on a spectrum, with descriptions coalescing under seven themes: altered emotion, compromised performance, disengagement, dissatisfaction, exhaustion, overexertion and feeling overwhelmed. Wellbeing was perceived to comprise personal and professional domains that interacted and were fuelled by an underlying ‘reservoir’. Both constructs were linked by the degree of a trainee's value fulfilment, with burnout occurring when a trainee's wellbeing reservoir was depleted.ConclusionsParticipants in this study characterised burnout and wellbeing as multifaceted, connected constructs. Given the complexity of these constructs, preventive interventions should target both person and workplace‐focused factors, with value fulfilment proposed as the basic change mechanism. A novel model that synthesises and advances previous research is offered based on these findings.
Unrecognized role of claudin‐10b in basolateral membrane infoldings of the thick ascending limb
Authors: Quintanova Catarina , Himmerkus Nina , Svendsen Samuel L. , von Schwerdtner Otto , Merkel Cosima , Pinckert Lennart , Mutig Kerim , Breiderhoff Tilman , Müller Dominik , Günzel Dorothee , Bleich Markus 
Published: August 2022

AbstractClaudin‐10b is an important component of the tight junction in the thick ascending limb (TAL) of Henle's loop and allows paracellular sodium transport. In immunofluorescence stainings, claudin‐10b–positive cells exhibited extensive extra staining of basolateral, column‐like structures. The precise localization and function have so far remained elusive. In isolated cortical TAL segments from C57BL/6J mice, kidney‐specific claudin‐10 knockout mice (cKO), and respective litter mates (WT), we investigated the localization and protein expression and function by fluorescence microscopy and electrophysiological measurements. Ultrastructural analysis of TAL in kidney sections was performed by electron microscopy. Claudin‐10b colocalized with the basolateral Na+‐K+ ATPase and the Cl– channel subunit barttin, but the lack of claudin‐10b did not influence the localization or abundance of these proteins. However, the accessibility of the basolateral infolded extracellular space to ouabain or fluorescein was increased by basolateral Ca2+ removal and in the absence of claudin‐10b. Ultrastructural analysis by electron microscopy revealed a widening of basolateral membrane infoldings in cKO in comparison to WT. We hypothesize that claudin‐10b shapes neighboring membrane invaginations by trans interaction to stabilize and facilitate high‐flux salt transport in a water‐tight epithelium.
Exploration of incompatible crosses in plants for novel and useful variations
Authors: Kurella B.R.S. Visarada , Jacob Jinu , Meena Kanti , Cheruku Deepika , Mulpuri Sujatha 
Published: August 2022

AbstractCrop improvement through pre‐breeding utilizes elite lines, germplasm and species of compatible gene pools, but crossing and characterizing the derivatives between incompatible species, especially distant genera or species, is realized to introduce de novo variations that are heritable, stable and especially economically contributing. This is a novel approach to introduce large‐scale de novo variations in crops that can be applied to widening of the genetic base. It helps to introduce heritable and agronomical variations that can be evolved in a short time for use in trait specific breeding programmes. In crosses between incompatible species many times F1 are delusive appearing like maternal parent. But large variations in F2 are observed due to genetic changes that include partial or complete elimination of the genome from the paternal parent, reshuffling of the maternal genome leading to several changes that are translated to phenotype. Genome, transcriptome and epigenetic reprogramming is responsible for the above genetic changes. Crossing two distant species can be used as an additional tool in plant breeding.
Evaluation of Detector Position and Light Fluence Distribution Using an Infrared Navigation System during Pleural Photodynamic Therapy†
Authors: Sun Hongjing , Kim Michele M. , Ong Yi Hong , Dimoft Andreea , Singhal Sunil , Busch Theresa M. , Cengel Keith A. , Zhu Timothy C. 
Published: August 2022

AbstractPhotodynamic therapy (PDT) has been used to treat malignant pleural mesothelioma. Current practice involves delivering light to a prescribed light fluence with a point source, monitored by eight isotropic detectors inside the pleural cavity. An infrared (IR) navigation system was used to track the location of the point source throughout the treatment. The recorded data were used to reconstruct the pleural cavity and calculate the light fluence to the whole cavity. An automatic algorithm was developed recently to calculate the detector positions based on recorded data within an hour. This algorithm was applied to patient case studies and the calculated results were compared to the measured positions, with an average difference of 2.5 cm. Calculated light fluence at calculated positions were compared to measured values. The differences between the calculated and measured light fluence were within 14% for all cases, with a fixed scattering constant and a dual correction method. Fluence‐surface histogram (FSH) was calculated for photofrin‐mediated PDT to be able to cover 80% of pleural surface area to 50 J cm−2(83.3% of 60 J cm−2). The study demonstrates that it will be possible to eliminate the manual measurement of the detector positions, reducing the patient's time under anesthesia.
Care coordination for healthcare referrals under a shared‐savings program
Authors: Bravo Fernanda , Levi Retsef , Perakis Georgia , Romero Gonzalo 
Published: August 2022

Accountable care organizations (ACOs) are responsible for the quality and cost of care of specified patient populations, including the cost of referrals. Motivated by this environment, we study care coordination for healthcare referrals. We consider an ACO that refers an uncertain number of patients from its attributed population to a preferred external provider for specialized health services. ACOs are typically paid under the Medicare Shared Savings Program (MSSP). Under the MSSP, the payer sets a spending benchmark for the beneficiary population during a fixed time period and shares any gains (losses) relative to it with the ACO. During the billing period, all services delivered to the attributed population by the ACO and external providers continue to be reimbursed under fee‐for‐service. Gains (losses) are determined at the end of the period by comparing the actual spending, which includes all care expenses (regular visits, referrals, and failed treatments) incurred by the payer in the period to the predefined benchmark. In this environment, the ACO and external providers—the latter not compensated under the MSSP—lack incentives to invest enough in care coordination initiatives. We study financial incentive mechanisms between the ACO and its preferred external provider to achieve integrated care coordination in referral markets under the MSSP. We show that traditional fee‐for‐service and capitation agreements do not provide sufficient incentives for care coordination in referral markets. However, a risk‐ and cost‐sharing mechanism can induce integrated care coordination efforts while satisfying the ACO and provider's participation constraints. We characterize a family of such mechanisms and numerically study the variability of the ACO and the external provider's profit. We demonstrate that this type of agreement can be used not only to induce integrated care coordination but can also result in a Pareto improvement in profit variability. We also illustrate the impact of the different MSSP risk tracks parameters on the performance of this care coordination mechanism, including their effect on the quality of care and the payer's mean spending.
Mitigating the financial risk behind emission cap compliance: A case in maritime transportation
Authors: Sun Qinghe , Chen Li , Chou Mabel C. , Meng Qiang 
Published: August 2022

The enforcement of ever‐stringent regulatory requirements capping emission limits is challenging the traditional operations of various transportation sectors. In maritime transportation, the recent regulation tightening fuel sulfur limits to 0.50%, known as the “IMO 2020,” has been enforced. There is a flurry of activities by ocean carriers to equip their vessels to comply with this regulation. Although the technical conditions are clear, investment decisions are hard to make due to inevitable uncertainties in the current transition period, especially on the impact of fuel prices in the long run. In this study, we consider an ocean carrier's technology investment decisions. Each compliance solution is subject to uncertain operating costs with a partially characterized probability distribution that may deviate from current expected norms. The carrier chooses a portfolio of compliance solutions for its entire fleet that would best adhere to two decision criteria characterized by a net present value (NPV) target in investment and a capacity utilization rate target in fleet deployment. To find optimal decisions that will perform well in the uncertain transition period, we introduce a tractable mathematical model, termed the ambiguous robustness optimization model, to minimize the financial riskiness index associated with the risk of expected NPV not meeting a specified target. We further propose a solution scheme through mixed‐integer second‐order cone programming approximation that can be efficiently solved by off‐the‐shelf solvers. We show that this decision support system performs well in numerical experiments constructed using real data on the Asia‐North America West Coast shipping network.
Liberalization for services foreign direct investment and product mix adjustment: Evidence from Chinese exporting firms
Authors: Bai Zhuoran , Meng Shuang , Miao Zhuang , Zhang Yan 
Published: August 2022

AbstractThis article is the first to explore how manufacturing firms adjust their export product mix in response to services liberalization. We focus on the liberalization of services foreign direct investment (FDI) in destination (importing) countries. Our analysis of detailed customs transaction data from China, finds a promotion effect of services FDI liberalization on export diversification of manufactured goods, which is mainly revealed by an increase in export value, variation in the number of product varieties, the dispersion ratio across exported products, and a decrease in the export skewness ratio. However, this diversification‐promotion effect is only found in developing countries, but not present in developed countries. Our study suggests that services liberalization incurred in importing countries play an important role in promoting export scale and diversification.
The OsWRKY63–OsWRKY76–OsDREB1B module regulates chilling tolerance in rice
Authors: Zhang Mingxing , Zhao Ranran , Huang Kai , Huang Shuangzhan , Wang Haitao , Wei Zhiqi , Li Zhao , Bian Mingdi , Jiang Wenzhu , Wu Tao , Du Xinglin 
Published: August 2022

SUMMARYRice (Oryza sativa) is sensitive to low temperatures, which affects the yield and quality of rice. Therefore, uncovering the molecular mechanisms behind chilling tolerance is a critical task for improving cold tolerance in rice cultivars. Here, we report that OsWRKY63, a WRKY transcription factor with an unknown function, negatively regulates chilling tolerance in rice. OsWRKY63‐overexpressing rice lines are more sensitive to cold stress. Conversely, OsWRKY63‐knockout mutants generated using a CRISPR/Cas9 genome editing approach exhibited increased chilling tolerance. OsWRKY63 was expressed in all rice tissues, and OsWRKY63 expression was induced under cold stress, dehydration stress, high salinity stress, and ABA treatment. OsWRKY63 localized in the nucleus plays a role as a transcription repressor and downregulates many cold stress‐related genes and reactive oxygen species scavenging‐related genes. Molecular, biochemical, and genetic assays showed that OsWRKY76 is a direct target gene of OsWRKY63 and that its expression is suppressed by OsWRKY63. OsWRKY76‐knockout lines had dramatically decreased cold tolerance, and the cold‐induced expression of five OsDREB1 genes was repressed. OsWRKY76 interacted with OsbHLH148, transactivating the expression of OsDREB1B to enhance chilling tolerance in rice. Thus, our study suggests that OsWRKY63 negatively regulates chilling tolerance through the OsWRKY63–OsWRKY76–OsDREB1B transcriptional regulatory cascade in rice.
Health care providers’ experiences caring for women living with obesity during pregnancy, birth, and postpartum: a qualitative systematic review protocol
Authors: Smith Helen , Macdonald Danielle , Ross-White Amanda , Grant Shannan , Snelgrove-Clarke Erna 
Published: August 2022

ABSTRACT

Objective:
This review will examine qualitative evidence about the experiences of health care providers who provide care to women living with obesity during the perinatal period to support evidence-informed approaches to care.

Introduction:
As the number of women living with obesity increases, health care providers are interacting more with this population during pregnancy, birth, and postpartum. Qualitative studies about this topic show that health care providers often face challenges that prevent the provision of quality care. A qualitative systematic review of the experiences of health care providers caring for women living with obesity throughout the perinatal period is important to identify and address current gaps in the delivery of perinatal health care services.

Inclusion criteria:
All studies with settings where licensed health care providers care for women living with obesity during pregnancy, birth, and postpartum will be considered. Studies published in English from 1995 onward will be included. The review will consider both mixed methods and qualitative studies such as phenomenology, grounded theory, and participatory action research.

Methods:
The following databases will be searched: CINAHL, Embase, PsycINFO, MEDLINE, and Sociological Abstracts (ProQuest). The ProQuest Digital Dissertations database will be searched for unpublished studies. Two independent reviewers will assess each study. Conflicts will be resolved through discussion. Data will be extracted by 2 trained independent reviewers. JBI resources for meta-aggregation will be used and data will be categorized and synthesized accordingly.
A Comprehensive 4E Study on the Performance of Bifacial Solar Module Installed on Different Ground Surface Colors: An Experimental Study on a Specific Site
Authors: Muthu Vimala , Ramadas Geetha 
Published: August 2022

Abstract
Solar energy will be the most sought-after source for generating electricity shortly because of its availability in abundance and pollution-free nature. Bifacial photovoltaic (PV) technology increases the power output through the albedo effect. However, the major drawback of PV-based power is that the efficiency is very low at less than 25%. The study focuses on the impact of surface color to explore the possibilities of enhancing the efficiency of solar modules considering the different terrace surfaces available in the residential region. The proposed work is one such attempt where the study is mainly focused on the impact of the surface properties on the extraction of electricity from the solar module without adopting the active techniques. A detailed study on different colors like black, green, and white is carried out. The study observed that the white surface improves the albedo effect toward the rear surface of the module, thereby improving the energy production factor (EPF) and higher life cycle conversion efficiency (LCE). It is observed that there is a 4.8% increase in the average efficiency when using white as ground cover as compared to normal reference ground. The comparative study is also carried out for various lifetime periods (T) like 10, 15, and 20 years. Calculated the exergetic cost by considering operating periods like 15, 20, 25, and 30 years with 2%, 5%, and 10% interest rates, and it is observed that after 30 years of operation at a 2% interest rate, energetic cost reached its highest value.
Physical Education Teachers’ Experiences of Nurturing a Community of Practice Online
Authors: Ferreira Heidi J. , Gonçalves Luiza , Parker Melissa 
Published: August 2022

Purpose: Drawing on the concept of community of practice, the purpose of this study was to explore Brazilian physical education teachers’ experiences of nurturing a community of practice online during the COVID-19 pandemic. Method: This was an action research project, and the participants were 13 high school physical education teachers (one of them acted as teacher-facilitator), one critical friend, and one meta-critical friend. Data sources included teachers’ online meetings, critical friend conversations, interviews, and the facilitator’s reflexive journal. Results: Inductive analysis resulted in four themes: finding emotional and pedagogical support, building a teachers-only space, creating an online group dynamic, and negotiating to research their own practice. The findings are discussed in relation to the characteristics of community of practices and online continuing professional development. Conclusion: Key aspects for future online collaborative continuing professional development are highlighted: (a) teachers-only; (b) by teachers, for teachers; and (c) combination of tools.