bluuebunny/crossref_metadata_2025_split huggingface dataset:
Considering gene therapy to protect from X‐linked deafness DFNX2 and associated neurodevelopmental disorders
Authors: Defourny Jean 
Published: September 2022

AbstractMutations and deletions in the gene or upstream of the gene encoding the POU3F4 transcription factor cause X‐linked progressive deafness DFNX2 and additional neurodevelopmental disorders in humans. Hearing loss can be purely sensorineural or mixed, that is, with both conductive and sensorineural components. Affected males show anatomical abnormalities of the inner ear, which are jointly defined as incomplete partition type III. Current approaches to improve hearing and speech skills of DFNX2 patients do not seem to be fully effective. Owing to inner ear malformations, cochlear implantation is surgically difficult and may predispose towards severe complications. Even in cases where implantation is safely performed, hearing and speech outcomes remain highly variable among patients. Mouse models for DFNX2 deafness revealed that sensorineural loss could arise from a dysfunction of spiral ligament fibrocytes in the lateral wall of the cochlea, which leads to reduced endocochlear potential. Highly positive endocochlear potential is critical for sensory hair cell mechanotransduction and hearing. In this context, here, we propose to develop a therapeutic approach in male Pou3f4−/y mice based on an adeno‐associated viral (AAV) vector‐mediated gene transfer in cochlear spiral ligament fibrocytes. Among a broad range of AAV vectors, AAV7 was found to show a strong tropism for the spiral ligament. Thus, we suggest that an AAV7‐mediated delivery of Pou3f4 complementary DNA in the spiral ligament of Pou3f4−/y mice could represent an attractive strategy to prevent fibrocyte degeneration and to restore normal cochlear functions and properties, including a positive endocochlear potential, before hearing loss progresses to profound deafness.
Sinomenine pretreatment alleviates hepatic ischemia/reperfusion injury through activating Nrf‐2/HO‐1 pathway
Authors: Hui Bo , Shu Yantao , Yang Dandan , Wang Zhidong , Zhang Li , Lei Nina , Yang Zhengan 
Published: September 2022

AbstractIntroductionIschemia–reperfusion (IR) injury is induced by an interrupted blood flow and succeeding blood restoration, which is common in the operation of liver transplantation. Serious IR injury is a major reason leading to transplant failure. Hepatic IR is featured by excessive inflammatory response, oxidative stress, and apoptosis. Sinomenine (SIN) is derived from the herb Sinomeniumacutum and shows properties of anti‐inflammation and antiapoptosis in multiple IR‐induced organ injuries. However, the effect of SIN in hepatic IR has not been investigated.MethodsThis study aims to investigate impacts of SIN on hepatic IR and the involved signaling pathway. An in vivo rat model of syngeneic orthotopic liver transplantation was constructed to induce the hepatic IR injury.ResultsResults showed that SIN pretreatment provided a significant prevention against IR‐induced hepatic injury as manifested by the downregulated activities of serum alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase, the alleviatedoxidative stress as shown by increased activities of serum superoxide dismutase and glutathione peroxidase, and decreased serum level of malondialdehyde, the suppressed inflammatory responses as shown by downregulated serum tumor necrosis factor‐α, interleukin (IL)‐6, IL‐8 levels, and upregulated IL‐10 level, as well as attenuated apoptosis as shown by decreased protein expression of cleaved caspase‐3 and −9. In line with these results, SIN pretreatment also alleviatedthe hepatic histopathological changes in IR rats and induced Nrf‐2/HO‐1 activation. The use of brusatol, a selective inhibitor for Nrf‐2, effectively reversed SIN‐induced above effects.ConclusionsAltogether, our results demonstrate that SIN might be a useful therapeutic drug for preventing hepatic IR‐induced injury during clinical liver transplantation.
Bedtime procrastination mediates the relation between anxiety and sleep problems
Authors: Campbell Rebecca L. , Bridges Ana J. 
Published: September 2022

AbstractThe prevalence of sleep problems is high in primary care patients and in anxious individuals. This study assessed whether total sleep time and bedtime procrastination mediated the association between anxiety and sleep problems. We predicted higher anxiety would be negatively associated with total sleep time and positively associated with bedtime procrastination and sleep problems, and these variables would statistically mediate the association between anxiety and sleep problems. Participants were 308 adult primary care patients, predominantly female (non‐Hispanic White = 158, Latinx = 111, mean age = 33.30), who initiated behavioral health services at an integrated primary care clinic. Patients completed a questionnaire regarding psychological health and sleep behaviors. Using structural equation modeling, we found higher anxiety related to higher sleep problems, partially mediated by bedtime procrastination but not total sleep time. This study highlights related factors like anxiety and prebedtime behaviors that may be effective treatment targets for sleep challenges.
Examining heterogeneity in mentoring: Associations between mentoring discussion topics and youth outcomes
Authors: Alwani Noor A. , Lyons Michael D. , Edwards Kelly D. 
Published: September 2022

AbstractThe current study aims to apply a staged approach to document heterogeneity in discussions in mentoring relationships, chiefly, discussion topics from weekly mentoring sessions with undergraduate women mentors (n = 40), then link each of the eight topics (relationships with friends, family, teachers, and romantic relationships, as well as goals, academic skills, academic problems, and hopes for the future) to developmental outcomes for middle school girls (n = 41) who participated in a school‐based mentoring program. In doing so, the authors hope to better understand the mechanisms that influence variability in mentoring treatment effects. Mentoring dyads engaged in unstructured one‐on‐one sessions and structured group meetings across the 2018−2019 academic year. The primary predictors for this study are weekly mentor‐reported discussion topics and activities addressed during unstructured one‐on‐one mentoring sessions, with 11 social‐emotional, academic, and behavioral outcomes measured via pre‐ and postsurveys administered by research assistants to mentees during the fall and spring. A series of 11 path analyses indicate small to moderate associations, both beneficial and negative, between key discussion topics, such as hopes for the future, family relationships, and goals, and several mentee‐reported outcomes of interest at the end of the intervention, including extrinsic motivation, life satisfaction, and self‐esteem. Study findings provide information about heterogeneity in mentoring practices to inform how various mechanisms of mentoring (e.g., discussions focused on relationships, goals and skills, and strengths) influence developmentally‐relevant effects for youth.
Gross, ultrastructural, and histological characterizations of pecten oculi of the glossy ibis (Plegadis falcinellus): New insights into its scanning electron microscope–energy dispersive X‐ray analysis
Authors: Kandyle Ramadan , El Basyouny Hebat Allah , Morsy Kareem , Abourashed Nagah M. , Madkour Naglaa , Abumandour Mohamed M. A. 
Published: September 2022

AbstractThe current study shows the first attempts to clarify the gross, ultrastructure, and histological properties of the pecten oculi of the diurnal, visually active glossy ibis, as well as scanning electron microscope–energy dispersive X‐ray (SEM–EDX) image analysis (Plegadis falcinellus). The pecten oculi was found on the eyeball's posteroinferior wall, adjacent to the optic nerve in both the craniocaudal and posterio‐anterior directions. The plicated quadrilateral black pigmented pecten oculi consisted of a base, 10–12 pleats, and an apex. The base was joined by an elevated ridge and derived from the non‐vascular retina, while the apex was not a fused part and was found neighboring the gelatinous vitreous body. The limited interpleat spaces were somewhat wider at the base of the pecten oculi. The outer surface of each highly vascular pecten pleat revealed a tortious coiled formation due to the existence of a considerable number of capillary vessels. The outer pleat surfaces had a vitreopecteneal limiting membrane that segregated the pecten pleats from the vitreous body. The high SEM magnification revealed that there are considerable plentiful hyalocyte cells on the outer surface of the pleats. The SEM–EDX analysis of the elementary formatting of the pecten oculi (at apex, middle, and base) clarified that the carbon represents the highest and a half percent. Furthermore, oxygen represents one‐third of all elemental composition in the three regions, while the lowest percentage is calcium. Finally, the pecten oculi characterizations of this migratory bird on the Northern Egyptian shore were associated with their adaptive dietary strategies.
Ernest Dichter's fur coat models: Fashioning a therapeutic culture
Authors: Rudeen Christopher M. 
Published: September 2022

AbstractDiscussions of the rise of therapeutic culture have tended toward the abstract, in part due to a focus on theory. This article looks at the case of Ernest Dichter's motivational research, particularly a study conducted on fur coats in the late 1950s, to locate this broader cultural shift more materially. Motivational research was a broad project of study that aimed to uncover unconscious consumer desires using the tools of psychology and psychoanalysis. This project materialized culture first through the pen‐and‐paper projective test created for the study, which sorted styles of fur into different classifications of womanhood, and second through the fur coats themselves, which were granted by Dichter a psychological agency of their own in their relationship with middle‐class women. Through this study, Dichter observed a shift in Americans' understanding of the self, a movement away from meeting physiological needs to addressing their inner lives; changing economic conditions had granted more income and free time with which to look inward, and Americans wanted consumer goods to aid in such self‐discovery. Dichter suggested that the fur industry capitalize on this change by emphasizing the versatility of fur and the role of objects more generally in fostering creative self‐expression. The advertising office was where theory was put into practice. In that way, it is a uniquely generative though often overlooked space from which to look into the rise of the therapeutic culture.
Fabrication and characterization of gelatin–EGCG–pectin ternary complex: formation mechanism, emulsion stability, and structure
Authors: Huang Xiang , Tu Rui , Song Hongbo , Dong Kai , Geng Fang , Chen Lei , Huang Qun , Wu Yingmei 
Published: September 2022

AbstractBACKGROUNDProtein–polyphenol–polysaccharide ternary complex particles have better emulsion interfacial stability compared to protein–polysaccharide binary complexes. However, knowledge is scarce when it comes to the fabrication of protein–polyphenol–polysaccharide ternary complexes as interfacial stabilizers and the interactions between the three substances. In the present work, ternary complexes were prepared using gelatin, high methoxyl pectin, and epigallocatechin gallate (EGCG) as raw materials. The effect of different influencing factors on the formation process of ternary complexes was investigated by varying different parameters. physicochemical stability, emulsifying properties, and structural characteristics were analyzed.RESULTSThe ternary complex had a smaller particle size (275 nm) and polydispersity index (0.112) when the mass concentration ratio of gelatin to high methoxyl pectin was 9:1, addition of EGCG was 0.05%, pH value was 3.0, and ionic strength was 10 mmol L−1. Meanwhile, the complex had the highest emulsifying stability index (691.75 min) and emulsifying activity index (22.96 m2 g−1). Scanning electron microscopical observation demonstrated that the addition of EGCG promoted the dispersion of ternary complex more uniformly, and effectively reduced the agglomeration phenomenon. The discrepancy in fluorescence intensity suggested that interactions between EGCG and gelatin occurred, which altered the protein spatial conformation of gelatin. Fourier transform infrared spectroscopic analysis elucidated that hydrogen bond interaction was the primary non‐covalent interaction between EGCG and gelatin–high methoxyl pectin binary complex.CONCLUSIONThe aforementioned results purposed to provide some theoretical reference and basis for the rational design of stable protein–polyphenol–polysaccharide ternary complexes. © 2022 Society of Chemical Industry.
Rapeseed peptide inhibits HepG2 cell proliferation by regulating the mitochondrial and P53 signaling pathways
Authors: Ma Keer , Wang Zhigao , Ju Xingrong , Huang Jiankang , He Rong 
Published: September 2022

AbstractBackgroundRapeseed peptide, extracted from rapeseed protein, is known to have a variety of biological activities. In this study, the anti‐proliferation effect and molecular mechanism of rapeseed peptide on HepG2 cells were investigated.ResultsIn vitro anticancer experiments showed that the rapeseed peptide NDGNQPL could inhibit HepG2 cell proliferation in a concentration‐dependent manner [half maximal inhibitory concentration (IC50), 1.56 mmol L−1). HepG2 cells were induced by NDGNQPL at a 0.5 mmol L−1 concentration and exhibited a 28.39 ± 0.80% apoptosis rate and a cell cycle arrest in the G0/G1 phase. Meanwhile, rapeseed peptide induced a decrease in mitochondrial membrane potential, an increase in reactive oxygen species (ROS) release, and changes in the nuclear morphology of HepG2 cells, indicating that rapeseed peptide could induce cell apoptosis through the mitochondrial pathway. In addition, rapeseed peptide activated the proliferation‐related P53 signaling pathway, in which the expression levels of P53, P21, and cleaved‐caspase3 were up‐regulated, while the expression levels of murine double minute 2 (MDM2) were down‐regulated. In molecular docking simulations, NDGNQPL exhibited a good affinity for the MDM2 molecule, which supported the notion that the rapeseed peptide is able to inhibit MDM2, a negative regulator of P53.ConclusionThe current results indicate that the rapeseed‐derived NDGNQPL peptide has the potential to inhibit the proliferation of HepG2 cells and promote human health. © 2022 Society of Chemical Industry.
Standard versus longer interval of radical resection after neoadjuvant chemoradiotherapy in locally advanced rectal cancer: A 20‐year single‐center experience & propensity‐score matching
Authors: Khamzina Saule , Lee Jongoh , Ryoo Seung‐Bum , Kim Min Jung , Park Ji Won , Kang Hyun‐Cheol , Chie Eui Kyu , Lee Dae‐Won , Han Sae‐Won , Kim Tae‐You , Jeong Seung‐Yong , Park Kyu Joo 
Published: September 2022

AbstractBackground and ObjectivesDespite the standard interval of 6−8 weeks between neoadjuvant chemoradiotherapy (nCRT) and surgery, it is debated whether an interval of >8 weeks increases the pathologic complete response (pCR) rate. We investigated the interval between nCRT and surgery, and its impact on oncological outcomes and postoperative complications in patients with locally advanced rectal cancer.MethodsWe retrospectively reviewed patients with rectal cancer who underwent total mesorectal excision after long‐course nCRT between 2000 and 2020. They were divided into two groups—those who underwent surgery at 6−8 and >8 weeks after nCRT. Surgical outcomes (stoma rate and postoperative complications), pCR, tumor regression grade (TRG), recurrence‐free survival (RFS), and overall survival (OS) were compared.ResultsWe selected 770/1153 patients with rectal cancer, including 502 and 268 patients surgically treated at 6−8 and >8 weeks after nCRT, respectively. The pCR rates were similar between the two groups (14.7% vs. 15.3%, p = 0.836), while the TRG was significantly better in the >8 weeks group (p = 0.267). Additionally, the postoperative complications, recurrence, 5‐year RFS, and OS rates were not significantly different between the two groups.ConclusionsAlthough tumor regression increased in the >8 weeks group, the oncological benefits of surgery >8 weeks after nCRT remain uncertain.
Curative‐intent treatment of pulmonary metastases from colorectal cancer: A comparison between imaging‐guided thermal ablation and surgery
Authors: Karam Elias , Tabutin Mayeul , Mastier Charles , Crignis Lucas De , Peyrat Patrice , Martin Valentine , Badon Floriane , Muller Xavier , Meeus Pierre , Rivoire Michel , Dupré Aurélien 
Published: September 2022

AbstractBackgroundPulmonary metastases (PM) are the most frequent extra‐abdominal metastases from colorectal cancer. Lung resection and imaging‐guided thermal ablation (IGTA) are used as curative‐intent treatment. We compared the outcomes of patients with PM, treated with resection or ablation.MethodsWe retrospectively analyzed data from patients who underwent surgery or IGTA for colorectal PM between April 2011 and November 2020. Surgery was performed for peripheral PM and IGTA for deep‐located PM not in contact with major vessels. Patients who had both procedures were excluded. Patients were compared using propensity score matching (PSM) analysis, stratified according to number, size, and unilaterality of PM.ResultsOne hundred and fourty‐six patients were included, 65 (44.5%) underwent surgery and 81 (55.5%) underwent IGTA. After PSM analysis, each group contained 46 patients. IGTA patients had a lower morbidity rate (13.1% vs. 15.2%, p = 0.028) and a shorter length of stay (5.13 vs. 2.63 days, p < 0.001). Oncological outcomes were similar in both groups with 5‐year OS of 80% and 5‐year progression‐free survival (PFS) of 30% (p = 0.657 and p = 0.504, respectively) with similar recurrence patterns.ConclusionLung resection and IGTA seem to have similar oncologic outcomes for both OS and PFS. IGTA could be an alternative effective treatment for small PM, whenever technically feasible.
Socio‐cultural impacts of peer‐to‐peer accommodation on host communities
Authors: Petruzzi Marina A. , Marques Catarina , Campos Ana Cláudia 
Published: September 2022

AbstractPeer‐to‐peer accommodation has recently emerged as a central focus of research in tourism and hospitality. However, research on socio‐cultural impacts of peer‐to‐peer accommodation is fragmented. This study reviews the relevant literature on the socio‐cultural impacts of peer‐to‐peer accommodation on host communities and proposes an integrative framework and a working definition for goal socio‐cultural impacts of peer‐to‐peer accommodation. This literature review contributes to knowledge of how this business paradigm affects and frames host communities and integrates the factors that should be explored by researchers, policymakers, and organizations to manage the impacts of peer‐to‐peer accommodation on host communities.
Experimental Preparation and Manipulation of Squeezed Cat States via an All‐Optical In‐Line Squeezer
Authors: Wang Meihong , Zhang Miao , Qin Zhongzhong , Zhang Qiang , Zeng Li , Su Xiaolong , Xie Changde , Peng Kunchi 
Published: September 2022

AbstractThe squeezed cat state, an essential quantum resource, can be used for quantum error correction and slowing decoherence of the optical cat state. However, preparing a squeezed cat state with high generation rate, and effectively manipulating it, remain challenging. In this work, a high‐performance all‐optical in‐line squeezer is developed to prepare a squeezed cat state and manipulate the phase of the quadrature squeezing. This scheme has the advantages that the phase of the quadrature squeezing of the squeezed cat state can be manipulated by changing the working condition of the squeezer, and that a higher generation rate can be achieved via the deterministic squeezing operation of the in‐line squeezer. The generation rate of squeezed cat states reaches 2 kHz, the same as that of the initial cat state. The all‐optical in‐line squeezer proposed here removes the requirements of electro‐optic and opto‐electric conversions necessary for an off‐line squeezer, thus enabling high‐bandwidth squeezing operations on non‐Gaussian states. These results provide an efficient method to prepare and manipulate optical squeezed cat states, which makes a step closer to their applications in all‐optical quantum information processing.
Nonlinear Chiroptical Holography with Pancharatnam–Berry Phase Controlled Plasmonic Metasurface
Authors: Wang Mingjie , Li Yang , Tang Yutao , Chen Jiafei , Rong Rong , Li Guixin , Cao Tun , Chen Shumei 
Published: September 2022

AbstractMetasurface optical holography represents an important technique for developing ultrathin diffractive optical elements. Among various designs, the metasurface devices with strong chiroptical responses offer a new degree of freedom to manipulate light fields. However, in a linear optical regime, it is usually complicated to realize strong chiroptical properties with metasurfaces. Here, nonlinear chiroptical holography using the Pancharatnam–Berry phase controlled achiral metasurface is reported. The metasurface consists of mirror symmetry breaking plasmonic meta‐atoms. Under the pumping of a fundamental wave, the achiral meta‐atoms radiate second harmonic waves with strong circular dichroism and spin dependent Pancharatnam–Berry phase. Both the amplitude and phase of the second harmonic waves can be independently and simultaneously controlled at the subwavelength scale. As a proof of concept, the spin selective nonlinear chiroptical holography is experimentally demonstrated through the second harmonic process on gold plasmonic metasurfaces. The proposed nonlinear metasurface with Pancharatnam–Berry phase and chiroptical properties open new avenues for multifunctional wavefront engineering and optical information processing.
Enriching captivity conditions with natural elements does not prevent the loss of wild‐like gut microbiota but shapes its compositional variation in two small mammals
Authors: Koziol Adam , Odriozola Iñaki , Nyholm Lasse , Leonard Aoife , San José Carlos , Pauperio Joana , Ferreira Clara , Hansen Anders J. , Aizpurua Ostaizka , Gilbert M. Thomas P. , Alberdi Antton 
Published: September 2022

AbstractAs continued growth in gut microbiota studies in captive and model animals elucidates the importance of their role in host biology, further pursuit of how to retain a wild‐like microbial community is becoming increasingly important to obtain representative results from captive animals. In this study, we assessed how the gut microbiota of two wild‐caught small mammals, namely Crocidura russula (Eulipotyphla, insectivore) and Apodemus sylvaticus (Rodentia, omnivore), changed when bringing them into captivity. We analyzed fecal samples of 15 A. sylvaticus and 21 C. russula, immediately after bringing them into captivity and 5 weeks later, spread over two housing treatments: a “natural” setup enriched with elements freshly collected from nature and a “laboratory” setup with sterile artificial elements. Through sequencing of the V3–V4 region of the 16S recombinant RNA gene, we found that the initial microbial diversity dropped during captivity in both species, regardless of treatment. Community composition underwent a change of similar magnitude in both species and under both treatments. However, we did observe that the temporal development of the gut microbiome took different trajectories (i.e., changed in different directions) under different treatments, particularly in C. russula, suggesting that C. russula may be more susceptible to environmental change. The results of this experiment do not support the use of microbially enriched environments to retain wild‐like microbial diversities and compositions, yet show that specific housing conditions can significantly affect the drift of microbial communities under captivity.