bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does surgical timing affect the rapidity of recovery in deep pain‐entire non‐ambulatory dogs with thoracolumbar intervertebral disk extrusion?
Authors: Vicens Zanoguera L. , Pauciulo C. , Corlazzoli D. , Cauduro A. , Motta L. 
Published: October 2022

ObjectiveTo investigate whether the delay between onset of neurological signs and spinal cord decompression affects the time to recovery in non‐ambulatory paraparetic/paraplegic dogs with deep pain perception affected by thoracolumbar intervertebral disc extrusion.Materials and MethodsData on non‐ambulatory dogs with preserved deep pain perception in both hindlimbs and surgically treated for thoracolumbar intervertebral disc extrusion were prospectively collected from three referral hospitals. Cox proportional hazards regression was used to explore the relationship of time until restoration of independent ambulation with age, weight, preoperative use of anti‐inflammatory drugs, delay between onset of inability to walk and arrival at the clinic, time between presentation and spinal surgery and surgery time.ResultsOne hundred and fifty‐one dogs were included. Median time from admission to surgery, including imaging, was 180 (interquartile range, 65.4 to 240) minutes. All dogs were operated within 72 hours. Follow‐up was available for all 151 dogs and ranged from 1 to 21 months. All but three dogs, which were all grade IV at presentation, recovered the ability to ambulate. In both univariable and multivariable models, only duration of surgery and neurological (Frankel) grade at presentation were significantly associated with the rapidity of recovery of ambulation.Clinical SignificanceDelay between onset of clinical signs and presentation and time between presentation and spinal surgery was not associated with the rapidity of recovery of ambulation in dogs surgically treated for thoracolumbar intervertebral disc extrusion. These results should be evaluated in the context of a limited range of delay since only 14.5% dogs had a delay ≥6.5 hours. Duration of surgery and neurological grade at presentation were significantly associated with rapidity of recovery of ambulation.
An integrative taxonomic study of Santolina (Asteraceae) from southern France and north‐eastern Spain reveals new endemic taxa
Authors: Giacò Antonio , Varaldo Lucia , Casazza Gabriele , De Luca Daniele , Caputo Paolo , Sarigu Marco , Bacchetta Gianluigi , Sáez Llorenç , Peruzzi Lorenzo 
Published: October 2022

AbstractSantolina is a clear example of a genus lying in an alpha‐taxonomic status, with species accepted only based on qualitative morphological descriptions. In particular, taxonomic issues still need to be resolved for Santolina populations from southern France and north‐eastern Spain, and so, we carried out an integrative taxonomic study involving morphometrics, cypsela morphometrics, niche overlap, and phylogenetic analysis based on six plastid markers (trnH‐psbA, trnL‐trnF, trnQ‐rps16, rps15‐ycf1, psbM‐trnD, and trnS‐trnG). Our results revealed that the current taxonomic circumscription is not adequate. In particular, the Santolina populations at the foothills of eastern Pyrenees, previously included in the variability of Santolina benthamiana, have to be considered as a distinct species, namely, Santolina intricata. In addition, despite their high phylogenetic relatedness, S. benthamiana s.str. and Santolina ericoides can still be considered as distinct species due to clear morphological and ecological differentiation. Finally, we demonstrated that three different subspecies can be recognized in Santolina decumbens, a species endemic to Provence. For one of these subspecies, due to its extremely restricted distribution range, conservation issues are pointed out.
Liver comparative transcriptome analysis reveals the mechanism of the Hainan medaka, Oryzias curvinotus, to adapt to salinity
Authors: Dong Zhongdian , Chen Yuebi , Li Huichen , Huan Zhang , Li Xueyou , Zhang Ning , Guo Yusong , Shao Changwei , Wang Zhongduo 
Published: October 2022

AbstractTo explore the adaptation mechanism of Hainan medaka, Oryzias curvinotus, to salinity, this study performed a histological and RNA‐Seq comparative analysis of female medaka liver with different salinities (0, 15 and 30 practical salinity unit [psu]). In the 30 psu group, the volume of hepatocytes was increased and cytoplasmic fat accumulated. A total of 83,758 unigenes were assembled by RNA‐Seq, of which 31,147 were annotated. In total, 922 differentially expressed genes (DEGs) were obtained, and the detection results of 24 DEGs by quantitative polymerase chain reaction (qPCR) were consistent with the RNA‐Seq data. Gene ontology (GO) enrichment showed that DEGs were mainly involved in biological processes, such as substance synthesis and transport, energy metabolism, signal transduction, and had catalytic activity and binding functions. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that DEGs were significantly enriched in steroid biosynthesis, glycerophospholipid metabolism, peroxisome proliferator‐activated receptor signaling pathway, and insulin signaling pathway that regulate lipid metabolism. The results of this study highlight the response of the liver transcriptome to long‐term environmental salinity changes and provide a basis for the application of the medaka genetic resources.
Population dynamics of recovering apex predators: Golden eagles in a Mediterranean landscape
Authors: Fernández‐Gil A. , Lamas J. A. , Ansola L. M. , Román J. , de Gabriel Hernando M. , Revilla E. 
Published: October 2022

AbstractApex predators play a critical role in shaping the biological and functional diversity of ecosystems. Like in many other living groups, population dynamics of apex predators exhibit auto‐regulation traits, including density‐dependent processes, which can be important for limiting population numbers. However, the study of these processes is challenging due to their slow life history traits, especially when their populations are depressed. Our main objective is to describe mechanisms driving population dynamics in apex predators by documenting the relationship between population density and demographic parameters at population level and analyzing the influence of population density and other environmental factors on the reproductive parameters at territory level. We used as biological model a recovering population of golden eagles Aquila chrysaetos in a Mediterranean landscape (North Spain). We monitored yearly all known eagle pairs within the study area for 28 years, implying 1539 reproductive events in a total of 84 territories. The average density was 3.04 pairs/1000 km2, and the reproductive success, productivity, and flight rates averaged 0.45, 0.54, and 1.20, respectively. The population increased during the study period (from 37 to 78 pairs), although we did not find any effect of density on the reproductive parameters at population level. At territory level, we found that size of territory, proportion of open habitat, and spring precipitation increased reproductive performance, while older territories performed worse than new ones. Our findings suggest that population dynamics in recovering apex‐predators are driven by a complex combination of compensatory density‐dependent processes, mainly operating at territorial level, and by environmental factors mainly related with resource availability and human pressure. For species with slow life history traits, population recovery seems to be facilitated by re‐colonization from refuge areas, wilder but less productive, to areas with higher resource availability, once they became safer after reduction of human pressures.
Epigenetic‐associated phenotypic plasticity of the ocean acidification‐acclimated edible oyster in the mariculture environment
Authors: Dang Xin , Lim Yong‐Kian , Li Yang , Roberts Steven B. , Li Li , Thiyagarajan Vengatesen 
Published: October 2022

AbstractFor marine invertebrates with a pelagic–benthic life cycle, larval exposure to ocean acidification (OA) can affect adult performance in response to another environmental stressor. This carry‐over effect has the potential to alter phenotypic traits. However, the molecular mechanisms that mediate “OA”‐triggered carry‐over effects have not been explored despite such information being key to improving species fitness and management strategies for aquafarming. This study integrated the genome‐wide DNA methylome and transcriptome to examine epigenetic modification‐mediated carry‐over OA impacts on phenotypic traits of the ecologically and commercially important oyster species Crassostrea hongkongensis under field conditions. Larvae of C. hongkongensis were exposed to control pH 8.0 and low pH 7.4 conditions, mimicking near future OA scenario in their habitat, before being outplanted as post‐metamorphic juveniles at two mariculture field sites with contrasting environmental stressors for 9 months. The larval carry‐over OA effect was found to have persistent impacts on the growth and survival trade‐off traits on the outplanted juveniles, although the beneficial or adverse effect depended on the environmental conditions at the outplanted sites. Site‐specific plasticity was demonstrated with a diverse DNA methylation‐associated gene expression profile, with signal transduction and the endocrine system being the most common and highly enriched functions. Highly methylated exons prevailed in the key genes related to general metabolic and endocytic responses and these genes are evolutionarily conserved in various marine invertebrates in response to OA. These results suggest that oysters with prior larval exposure history to OA had the ability to trigger rapid local adaptive responses via epigenetic modification to cope with multiple stressors in the field.
Influence of blood meal source on the biological parameters ofTriatoma pallidipennis(Heteroptera: Reduviidae) from Mexico
Authors: Martínez‐Ibarra José Alejandro , Nogueda‐Torres Benjamín , Montañez‐Valdez Oziel Dante , Michel‐Parra J. Guadalupe 
Published: October 2022

AbstractChagas disease is one of the most important vector‐borne diseases in Latin America, including Mexico.Triatoma pallidipennis(Stål) (Hemiptera: Reduviidae) is a Mexican triatomine vector commonly associated with different hosts. The influence of six blood meals (rabbits, rats, mice, dogs, cats and chickens) on six biological parameters of the biology ofT. pallidipenniswas evaluated. A significant difference was found in the period of egg‐to‐adult development between the five mammalian feeds (mean 195 days) and the chicken feed (221 days). The probability of survival was significantly lower in the chicken cohort (0.285). The total number of blood meals to moult from the first instar to the adult stage was the highest in the chicken cohort (10–15). This cohort had the significantly highest rate of females at the end cycle. The mean number of eggs laid per female and the egg eclosion rate were similar among the six food sources. Most results seemed to be influenced by the higher nutritional quality of the mammalian blood compared to the bird's blood and the increased energy expenditure required for the digestion of bird blood. These results clearly show thatT. pallidipennis, unlike other triatomine species, has a high reproductive capacity when feeding on different hosts.
A Lock‐and‐Kill Anticancer Photoactivated Chemotherapy Agent†
Authors: van Geest Erik Pieter , Götzfried Sina Katharina , Klein David M. , Salitra Nadiya , Popal Sorraya , Husiev Yurii , Van der Griend Corjan J. , Zhou Xuequan , Siegler Maxime A. , Schneider Gregory F. , Bonnet Sylvestre 
Published: October 2022

AbstractPhotosubstitutionally active ruthenium complexes show high potential as prodrugs for the photoactivated chemotherapy (PACT) treatment of tumors. One of the problems in PACT is that the localization of the ruthenium compound is hard to trace. Here, a ruthenium PACT prodrug, [Ru(3)(biq)(STF‐31)](PF6)2 (where 3 = 3‐(([2,2′:6′,2″‐ter‐ pyridin]‐4′‐yloxy)propyl‐4‐(pyren‐1‐yl)butanoate) and biq = 2,2′‐biquinoline), has been prepared, in which a pyrene tracker is attached via an ester bond. The proximity between the fluorophore and the ruthenium center leads to fluorescence quenching. Upon intracellular hydrolysis of the ester linkage, however, the fluorescence of the pyrene moiety is recovered, thus demonstrating prodrug cellular uptake. Further light irradiation of this molecule liberates by photosubstitution STF‐31, a known cytotoxic nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, as well as singlet oxygen via excitation of the free pyrene chromophore. The dark and light cytotoxicity of the prodrug, embedded in liposomes, as well as the appearance of blue emission upon uptake, were evaluated in A375 human skin melanoma cells. The cytotoxicity of the liposome‐embedded prodrug was indeed increased by light irradiation. This work realizes an in vitro proof‐of‐concept of the lock‐and‐kill principle, which may ultimately be used to design strategies aimed at knowing where and when light irradiation should be realized in vivo.
Investigating the defoliating‐like (DL) VCG2A pathotype of Verticillium dahliae through identification and prediction of secreted proteins from genomes of Australian isolates
Authors: Webster John , Dadd‐Daigle Pearl , Chapman Toni A. , Kirkby Karen 
Published: October 2022

AbstractIn Australia, recent investigations have reported the presence of a number of Verticillium dahliae pathotypes (VCG1A, VCG2A and VCG6) that cause disease in cotton and agricultural weeds. During these investigations, it was observed that a VCG2A V. dahliae displayed greater virulence than previously reported in local and international isolates. Genome sequencing of seven V. dahliae isolates was performed using a mixture of short and long reads sequencing technologies. Of these seven isolates, four were identified as D VCG1A, one ND VCG2A, a recently identified Australian VCG6 as well as a virulent VCG2A, classified as “defoliating‐like”. The secreted protein repertoire from the genomes of these Australian V. dahliae isolates was predicted using four separate signal prediction methods. The consensus secreted protein set of the isolates revealed the presence of 20 proteins that were present in all virulent isolates, including the virulent DL VCG2A, and absent in the nonvirulent VCG2A isolates. Ten of these proteins had a functional annotation and were identified as ligninase H8, lipolytic enzyme, laccase, amine oxidase, spherulin‐1A, dipeptidyl‐peptidase, monooxygenase, dienelactone hydrolase, carboxypeptidase and fibronectin by BLASTP.
A caregiver's perspective on clinically relevant symptoms in behavioural variant frontotemporal dementia: tools for disease management and trial design
Authors: Fieldhouse Jay L.P. , van Dijk Gaby , Gillissen Freek , van Engelen Marie‐Paule E. , de Boer Sterre C.M. , Dols Annemiek , van der Waal Hendrik‐Jan , Regeer Barbara J. , Vijverberg Everard G.B. , Pijnenburg Yolande A.L. 
Published: October 2022

ABSTRACTBackgroundAdequate detection of symptoms and disease progression in behavioural variant frontotemporal dementia (bvFTD) is complex. Dementia cohorts usually utilize cognitive and functional measures, which fail to detect dominant behavioural and social cognitive deficits in bvFTD. Moreover, since patients typically have a loss of insight, caregivers are important informants. This is the first qualitative study to investigate caregiver relevant symptoms during the disease course of bvFTD, aiming to improve tools for diagnosis, progression, and future clinical trials.MethodsInformal caregivers of patients in different disease stages of bvFTD (N = 20) were recruited from the neurology outpatient clinic of the Amsterdam UMC and a patient organization for peer support in the Netherlands. Their perspectives on clinical relevance were thoroughly explored during individual semi‐structured interviews. Inductive content analysis with open coding was performed by two researchers independently to establish overarching themes and patterns.ResultsCaregivers reported a variety of symptoms, in which (i) loss of emotional connection, (ii) preoccupation and restlessness, and (iii) apathy and dependency compose major themes of relevance for diagnosis and treatment. Within heterogeneous disease trajectories, symptom presence differed between stages and among individuals, which is relevant in the context of progression and outcome measures. Significant socio‐emotional changes dominated in early stages, while severe cognitive, behavioural, and physical deterioration shifted focus from predominant personality change to quality of life in later stages.ConclusionsCaregiver perspectives on target symptoms in bvFTD differ according to clinical stage and patient‐caregiver characteristics, with significant socio‐emotional changes characterizing early stages. These findings call for more appropriate tools and symptomatic treatments, as well as a personalized approach in treatment of bvFTD and a focus on early stage interventions in clinical trial design.
Validation of new, gender‐neutral questions on recent sexual behaviors among plasma donors and men who have sex with men
Authors: Lewin Antoine , Renaud Christian , Germain Marc , Boivin Amélie , Thibeault Catherine , Caruso Jessica , Otis Joanne 
Published: October 2022

AbstractBackground and ObjectivesSeveral blood services might eventually interview donors with gender‐neutral questions on sexual behaviors to improve the inclusivity of blood donation. We tested two ways (i.e., “scenarios”) of asking donors about their recent sexual behaviors.Materials and MethodsThe study comprised 126 regular source plasma donors and 102 gay, bisexual, and other men who have sex with men (gbMSM), including 73 cis‐gbMSM (i.e., the “cis‐gbMSM subgroup,” which excluded nonbinary, genderqueer, and trans individuals). In Scenario 1, participants were asked if, in the last 3 months, they “have […] had a new sexual partner or more than one sexual partner.” In Scenario 2, they were asked “Have you had a new sexual partner?” and “have you had more than one sexual partner?”. Validation questions included more specific questions on the type of partners and sexual activity.ResultsAmong plasma donors, sensitivity was 100.0% for both scenarios; specificity was 100.0% and 99.1% for Scenarios 1 and 2, respectively. Among gbMSM, sensitivity was 74.5% and 82.9% for Scenarios 1 and 2, respectively; specificity was 100.0% for both scenarios. Among cis‐gbMSM, sensitivity was 88.6% and 100.0% for Scenarios 1 and 2, respectively; specificity was 100.0% for both scenarios. The area under the receiver operating characteristic curve of Scenario 2 was significantly higher than that of Scenario 1 among gbMSM and in the cis‐gbMSM subgroup (all p < .05).ConclusionScenario 2 questions performed well among plasma donors and cis‐gbMSM, but less so in the broader gbMSM population.
A multimodal approach to English for academic purposes in contexts of diversity
Authors: Archer Arlene 
Published: October 2022

AbstractThis article focuses on the affordances of multimodal pedagogies in teaching English for academic purposes (EAP) in diverse and multilingual contexts. It draws on South Africa as an example of a multilingual, culturally diverse site in a recently decolonized country. Here theorizing and pedagogical practices around EAP often have a social justice agenda in order to redress the inequalities of the past. The importance of recognizing the multilingual and multimodal resources of students is highlighted in the teaching of academic writing. To this end, the article examines talk as an important mode in improving writing, drawing on multilingualism as a resource, working on screen versus page, the affordances of mind‐mapping and visualizing academic argument. The contention is that multimodal pedagogies can acknowledge students as agentive and creative meaning‐makers. This is particularly relevant in a context in which autonomous and decontextualized models of student support persist and students continue to be constructed as ‘lacking’ in resources.
Antegrade left ureterolithotripsy in a patient with previous psoas-hitch ureteral reimplantation
Authors: Colalillo Gaia , Annino Filippo , Khorrami Saba , Asimakopoulos Anastasios D 
Published: October 2022

A standard surgical treatment of distal ureteric defects is represented by the ureteroneocystostomy-ureteric reimplantation. However, the procedure involves an anatomical alteration of the ureterovesical (neo)junction that often hinders the retrograde catheterisation of the reimplanted ureter.We describe a case of antegrade ureterolithotripsy (AULT) in a psoas-hitch reimplanted ureter. A woman with severe left hydronephrosis supported by a subcentimetric proximal ureteral stone in a psoas-hitch reimplanted ureter was referred to our unit. Retrograde ureteroscopy was unsuccessful due to impossibility in incannulating the ureteral neo-orifice. Following the placement of a percutaneous nephrostomy, percutaneous AULT through ureteral sheath was successfully performed with complete treatment of the stone.AULT may represent a viable alternative in the management of ureteral stones when the upper urinary tract is not amenable to retrograde ureteroscopy. In experienced hands, the procedure is straightforward and may avoid the adoption of transabdominal approaches.
Adolescent and young adult allergic asthma treatment challenges
Authors: Vukičević Lazarević Vesna , Marković Ivan , Šola Ana Marija 
Published: October 2022

The transition from paediatric care to adult care is often difficult, especially in children with chronic diseases like asthma. A significant number of children reach remission throughout puberty; consequently, they are not tracked down for subsequent follow-ups and are not included in transition programmes to adult care. This case report focuses on a young adult with asthma that began in childhood and went into remission during adolescence, only to experience a recurrence when the patient was a young adult. Due to failing to complete the transition process into adult care services, she had poor adherence to therapy and asthma control.Adherence and asthma control significantly improved after a multidisciplinary approach in an adult care setting. In conclusion, appropriate transition and a multidisciplinary approach are critical for the effective management of asthma in young adults.