bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro Effect of 810 nm and 940 nm Diode Laser Irradiation on Proliferation of Human Gingival Fibroblasts and Expression of Procollagen Gene
Authors: Pourshahidi Sara , Ebrahimi Hooman , Bahrami Naghmeh , Abbasi Javan Zahra , Ghoreyshi Younes , Chiniforush Nasim , Kharazifard Mohammad Javad 
Published: April 2022

ABSTRACTFactors promoting fibroblast proliferation and collagen synthesis can subsequently enhance wound healing. This study aimed to assess the effect of 810 and 940 nm diode laser on fibroblast proliferation and procollagen gene expression. In this study, human gingival fibroblasts were cultured in Dulbecco’s modified Eagle’s medium and underwent 810 and 940 nm diode laser irradiation once, twice, thrice and four times at 1, 3, 5 and 7 days after culture. The methyl thiazolyl tetrazolium assay was performed to assess the proliferation while the real‐time polymerase chain reaction was performed to assess the expression of procollagen gene at the mRNA level. We applied two‐way ANOVA and Tukey’s test for analysis. Wavelength had no significant effect on the proliferation of gingival fibroblasts, but increasing the number of irradiation sessions of both wavelengths increased the proliferation of human gingival fibroblasts. Significant differences were noted in the number of human gingival fibroblasts between groups irradiated 1 and 4 and also 2 and 4 times. Procollagen gene was well expressed in all groups but its expression was significantly higher in 940 nm laser group after four irradiation cycles. Four times radiation of 940 nm laser seems to be more effective than all others.
Ectopic expression of BrIQD35 promotes drought stress tolerance in Nicotiana benthamiana
Authors: Yuan J. , Yu Z. , Li Y. , Shah S. H. A. , Xiao D. , Hou X. , Li Y. 
Published: April 2022

ABSTRACT

The plant IQD gene family is responsive to a variety of stresses. In this study, we studied the structural features and functions of the gene BrIQD35 in Chinese cabbage, a member of the IQD gene family.

BrIQD35 was cloned and shown to contain an IQ motif. Transient expression of BrIQD35 indicated that it was localized on the plasma membrane and was significantly upregulated under drought and salt stress in Chinese cabbage. To further identify the function of BrIQD35, it was heterologously overexpressed in Nicotiana benthamiana. Although there was no significant difference between BrIQD35‐overexpressed and wild‐type (WT) plants under salt stress, WT N. benthamiana showed more wilting than the BrIQD35‐overexpressed plants under drought stress.

Since the IQ motif has been annotated as a CaM binding site, yeast two‐hybrid assays were used to explore the interaction between BrIQD35 and CaM. The results indicated that BrIQD35 interacts weakly with CaMb, but not with CaMa, suggesting that BrIQD35 may function through the Ca2+‐CaMb pathway.

The findings reveal a novel gene involved in drought tolerance, which is important for plant breeding and quality improvement for Chinese cabbage.
Peroxisome proliferator‐activated receptor expression in the canine endometrium with cystic endometrial hyperplasia–pyometra complex
Authors: Socha Barbara M. , Socha Piotr , Szóstek‐Mioduchowska Anna Z. , Nowak Tomasz , Skarżyński Dariusz J. 
Published: April 2022

AbstractThe most common uterine diseases in bitches occurring during diestrus are cystic endometrial hyperplasia (CEH) and pyometra. These diseases can coexist as CEH–pyometra complex (CEH‐P). Their pathogenesis has not been fully explained. Peroxisome proliferator‐activated receptors (PPARs) are important factors regulating mammalian reproductive function and inflammatory processes. Although there is a lack of data concerning the expression of PPARs in the canine endometrium during CEH and CEH‐P, we hypothesized that they might be involved in the development of pathological disorders of the canine endometrium. Therefore, the current study was conducted to evaluate and compare PPARα, PPARδ and PPARγ mRNA expression using quantitative real‐time PCR (qPCR) and their immunolocalization using immunohistochemistry (IHC) staining in the endometrium of clinically healthy bitches (control group; n = 8) and those with CEH (n = 8) or CEH‐P (n = 8). For quantification, the arithmetic means of all intensities of immunostaining from the cells were measured with the optical density. PPARα, PPARδ and PPARγ were detected in the luminal epithelium, glandular epithelium and stromal cells. The mRNA transcription of PPARα was higher in the CEH group than in the control group (p < .05). Additionally, the mRNA expression and immunostaining intensities of PPARδ and PPARγ in the endometrium in the CEH‐P group were downregulated relative to those in the control group (p < .05). Moreover, the serum progesterone concentration measured by direct radioimmunoassay was decreased in the CEH‐P group compared to the control group (p < .001) and CEH group (p < .05). The obtained results indicate that PPARs are present in the canine endometrium and that their mRNA profile and intensity levels change under pathological conditions such as CEH and CEH‐P. This finding may suggest a correlation between changes in the PPAR expression profile and hormonal disturbances, as well as the potential involvement of PPARs in signal transduction during inflammatory processes occurring in the endometrium during CEH‐P. These results pave the way to further research into the role of PPARs in the pathogenesis of CEH and CEH‐P in female dogs.
Financial inclusion and education: An empirical study of financial inclusion in the face of the pandemic emergency due to Covid‐19 in Latin America and the Caribbean
Authors: Kazemikhasragh Amirreza , Buoni Pineda Marianna Vanessa 
Published: April 2022

AbstractFinancial inclusion and education contribute to a country's development and economic growth. However, despite the significant efforts being made to increase access to financial products for women, a high percentage still do not have access to and effective use of formal financial services in the countries of Latin America and the Caribbean. This study analyzes financial inclusion (based on gender equality) in the countries studied using a pooled‐panel ordinary least squares econometric technique. Furthermore, the impact of interactions between the level of study, use of technology, academic degree during the Covid‐19 restrictions, number of credit borrowers, and number of borrowers with the interaction of the restrictions during the health emergency was evaluated employing the Gini coefficient and human development index (HDI). This study confirms that Latin America and the Caribbean countries can increase financial inclusion by changing their social aspects based on gender equality to ease using technology and access to credit. The results of this study are helpful for policy‐makers in formulating and implementing policies that lead to action plans that reverse an exclusionary financial system, promote financial education, and empower women.
Special report from the field: The women of the Islamic State in Iraq
Authors: Schulman Susan 
Published: April 2022

AbstractThere are a great many unaddressed grievances that persist in Iraq to this day – grievances whose roots reach back to the overthrow of Saddam Hussein in 2003 and which ultimately led to the creation of the Islamic State in Iraq and Syria (ISIS) in 2014. Even though the Islamic State was ousted in 2017, these grievances have not gone away. In this special report from the field, American photo‐journalist Susan Schulman presents the distressing testimony and images of the families that once supported the Islamic State in Iraq and who are now shunned by Iraqi society as a result.
Multigene phylogeny of blister beetles (Coleoptera, Meloidae) reveals extensive polyphyly of the tribe Lyttini and allows redefining its boundaries
Authors: Riccieri Alessandra , Mancini Emiliano , Pitzalis Monica , Salvi Daniele , Bologna Marco A. 
Published: April 2022

AbstractMeloidae is a beetle family with about 3000 species divided into three subfamilies and 16 tribes. Included is the tribe Lyttini containing 33 genera and about 400 species. However, the boundaries of Lyttini have proved hard to define since the systematics of this tribe have repeatedly changed. In this study, we investigated the phylogeny of Lyttini and tested for its monophyly based on a comprehensive molecular dataset of mitochondrial (COI and 16S) and nuclear (CAD and 28S) genes including 57 genera and 385 species representing most of the tribes of Meloidae. Further phylogenetic analyses were performed on a reduced taxon set (51 genera and 135 species covering 60% of the generic diversity of Lyttini) in order to maximize the number of loci and minimize missing data. Phylogenetic results strongly supported the monophyly of most of the tribes of the subfamily Meloinae, apart from Lyttini, which is split into 11 phylogenetically distinct lineages scattered throughout the tree. Based on these results, we restrict the tribe Lyttini to the genera Alosimus Mulsant, Lydus Dejan, Lytta Fabricius, Muzimes Mulsant & Rey and Oenas Latreille, all exclusive to the Holarctic region. The relationships among the remaining genera previously included in Lyttini and the other 10 lineages representing ‘non‐Lyttini’ tribes remain to be clarified. This study also provides a number of nomenclatural acts [including Pseudolytta n. stat.; Lytta (Taurilytta) n. subgen.; Lytta (Teratolytta) n.stat.; Mimovesperus resurrected; Denierella = Epicauta n.syn.; Psalydolytta = Epicauta n. syn.] and working hypotheses for future investigations on the systematics of Meloidae.
A New Method for Quantifying Abdominal Aortic Wall Shear Stress Using Phase Contrast Magnetic Resonance Imaging and the Womersley Solution
Authors: Iffrig Elizabeth , Timmins Lucas H. , El Sayed Retta , Taylor W. Robert , Oshinski John N. 
Published: April 2022

Abstract
Wall shear stress (WSS) is an important mediator of cardiovascular pathologies and there is a need for its reliable evaluation as a potential prognostic indicator. The purpose of this work was to develop a method that quantifies WSS from two-dimensional (2D) phase contrast magnetic resonance (PCMR) imaging derived flow waveforms, apply this method to PCMR data acquired in the abdominal aorta of healthy volunteers, and to compare PCMR-derived WSS values to values predicted from a computational fluid dynamics (CFD) simulation. The method uses PCMR-derived flow versus time waveforms constrained by the Womersley solution for pulsatile flow in a cylindrical tube. The method was evaluated for sensitivity to input parameters, intrastudy repeatability and was compared with results from a patient-specific CFD simulation. 2D-PCMR data were acquired in the aortas of healthy men (n = 12) and women (n = 15) and time-averaged WSS (TAWSS) was compared. Agreement was observed when comparing TAWSS between CFD and the PCMR flow-based method with a correlation coefficient of 0.88 (CFD: 15.0 ± 1.9 versus MRI: 13.5 ± 2.4 dyn/cm2) though comparison of WSS values between the PCMR-based method and CFD predictions indicate that the PCMR method underestimated instantaneous WSS by 3.7 ± 7.6 dyn/cm2. We found no significant difference in TAWSS magnitude between the sexes; 8.19 ± 2.25 versus 8.07 ± 1.71 dyn/cm2, p = 0.16 for men and women, respectively.
Effects of exercise on fitness in adults with intellectual disability: a protocol of an overview of systematic reviews
Authors: Simón-Siles Sandra , Font-Farré Manel , Guerra-Balic Myriam , Nishishinya-Aquino Maria Betina , Oviedo Guillermo R 
Published: April 2022

IntroductionAdults with intellectual disability (ID) have lower physical fitness levels than their peers without disabilities, representing a risk to their health since physical activity and cardiorespiratory fitness are directly related to better health and quality of life. Therefore, it is essential to determine the effects that exercise can have on them, as adults with ID present high comorbidities and lower life expectancy, altogether with lower rates of physical activity. The current overview of systematic reviews aims to provide an outline of the exercise benefits in health-related and skill-related fitness in adults with ID.Methods and analysisResearch will be conducted in PubMed, CENTRAL, EMBASE, PEDro, SPORTDiscus and CINAHL. The search terms will be categorised through population (eg, adult, ID); intervention (eg, exercise, physical activity) and outcomes (eg, cardiorespiratory fitness, body composition, muscular strength, muscular endurance, flexibility, balance, power, speed, agility, coordination, mobility and reaction time). Each database will be searched from their earliest available record up to 30 September 2021.Inclusion criteria will be: systematic reviews including at least one RCT that compare exercise interventions with a control group or another type of intervention; measure of fitness using objectives methods; inclusion of adults with ID (≥18 years old), and published in any language, with at least their abstract in English, Spanish, French and/or Portuguese.Ethics and disseminationTo our knowledge, our overview will be the first of its kind to address the topic in people with ID. The results could be used to determine which fitness components can be improved by exercise and to provide a valuable tool to develop comprehensive exercise programmes specific to people with ID. Ethical approval is not required. The knowledge generated will be disseminated electronically and in print and presented at conferences.PROSPERO registration numberCRD42021237580.
Diagnostic and prognostic factors in patients with prostate cancer: a systematic review
Authors: Beyer Katharina , Moris Lisa , Lardas Michael , Haire Anna , Barletta Francesco , Scuderi Simone , Molnar Megan , Herrera Ronald , Rauf Abdul , Campi Riccardo , Greco Isabella , Shiranov Kirill , Dabestani Saeed , van den Broeck Thomas , Arun Sujenthiran , Gacci Mauro , Gandaglia Giorgio , Omar Muhammad Imran , MacLennan Steven , Roobol Monique J , Farahmand Bahman , Vradi Eleni , Devecseri Zsuzsanna , Asiimwe Alex , Zong Jihong , Maclennan Sara J , Collette Laurence , NDow James , Briganti Alberto , Bjartell Anders , Van Hemelrijck Mieke 
Published: April 2022

ObjectivesAs part of the PIONEER Consortium objectives, we have explored which diagnostic and prognostic factors (DPFs) are available in relation to our previously defined clinician and patient-reported outcomes for prostate cancer (PCa).DesignWe performed a systematic review to identify validated and non-validated studies.Data sourcesMEDLINE, Embase and the Cochrane Library were searched on 21 January 2020.Eligibility criteriaOnly quantitative studies were included. Single studies with fewer than 50 participants, published before 2014 and looking at outcomes which are not prioritised in the PIONEER core outcome set were excluded.Data extraction and synthesisAfter initial screening, we extracted data following the Checklist for Critical Appraisal and Data Extraction for Systematic Reviews of prognostic factor studies (CHARMS-PF) criteria and discussed the identified factors with a multidisciplinary expert group. The quality of the included papers was scored for applicability and risk of bias using validated tools such as PROBAST, Quality in Prognostic Studies and Quality Assessment of Diagnostic Accuracy Studies 2.ResultsThe search identified 6604 studies, from which 489 DPFs were included. Sixty-four of those were internally or externally validated. However, only three studies on diagnostic and seven studies on prognostic factors had a low risk of bias and a low risk concerning applicability.ConclusionMost of the DPFs identified require additional evaluation and validation in properly designed studies before they can be recommended for use in clinical practice. The PIONEER online search tool for DPFs for PCa will enable researchers to understand the quality of the current research and help them design future studies.Ethics and disseminationThere are no ethical implications.
Assessment of level of care recommendations and nursing acuity scores following an appropriateness of care intervention
Authors: Ruiz Colon Gabriela D , Sullivan K Michaela , Albaniel Moses , Britt Patricia , Shieh Lisa 
Published: April 2022

BackgroundAs part of a multiprong intervention to eliminate waste in cost of hospital accommodations, the InterQual Level of Care (LOC) criteria was deployed by our institution to assign patients to one of three LOCs: acute care, intermediate intensive care unit (IICU) or intensive care unit (ICU). In that intervention, which sought to decrease the number of patients in a higher LOC than what was clinically necessary, patient safety balancing metrics were stable. However, nursing workload, a key balancing metric, has yet to be examined. In this study, we examine nursing workload before and after the intervention using a proprietary nursing acuity score.MethodsA retrospective study was conducted analysing admissions at the study institution. Patient’s LOC recommendation (as determined by InterQual), assigned (actual) LOC and nursing acuity scores were collected and analysed. Average nursing acuity scores were compared across patients whose InterQual recommendation aligned with actual LOC (‘Acute Match’ or ‘IICU Match’) versus patients who were recommended to be in acute care but were receiving IICU care (‘Mismatch’).ResultsFollowing the intervention, the per cent of patients in the Mismatch cohort decreased from 13% to 7%. Prior to the intervention, average nursing acuity score for the Mismatch cohort was less than the IICU Match cohort and greater than Acute Match cohort in all departments analysed. After the intervention period, average acuity score in the Mismatch cohort exceeded that of the Acute Match cohort in all eight departments, but the Mismatch cohort’s scores differed from the IICU Match cohort in only one department.ConclusionCollectively, this study demonstrates that our intervention successfully decreased inappropriate use of the IICU LOC, and that the residual Mismatch cohort is a distinct entity, with nursing needs that exceed that of the Acute Match cohort. Thus, a higher LOC can be justified. This demonstrates that a nursing workload metric such as the nursing acuity score can be a valuable complement to clinical criteria such as the InterQual LOC criteria to objectively determine patient’s true, necessary LOC and ensure that nursing staff feels adequately staffed to care for patients.
Development of a sensitive trial-ready poly(GP) CSF biomarker assay forC9orf72-associated frontotemporal dementia and amyotrophic lateral sclerosis
Authors: Wilson Katherine M , Katona Eszter , Glaria Idoia , Carcolé Mireia , Swift Imogen J , Sogorb-Esteve Aitana , Heller Carolin , Bouzigues Arabella , Heslegrave Amanda J , Keshavan Ashvini , Knowles Kathryn , Patil Saurabh , Mohapatra Susovan , Liu Yuanjing , Goyal Jaya , Sanchez-Valle Raquel , Laforce Robert Jr , Synofzik Matthis , Rowe James B , Finger Elizabeth , Vandenberghe Rik , Butler Christopher R , Gerhard Alexander , Van Swieten John C , Seelaar Harro , Borroni Barbara , Galimberti Daniela , de Mendonça Alexandre , Masellis Mario , Tartaglia M Carmela , Otto Markus , Graff Caroline , Ducharme Simon , Schott Jonathan M , Malaspina Andrea , Zetterberg Henrik , Boyanapalli Ramakrishna , Rohrer Jonathan D , Isaacs Adrian M 
Published: April 2022

ObjectiveA GGGGCC repeat expansion in theC9orf72gene is the most common cause of genetic frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). As potential therapies targeting the repeat expansion are now entering clinical trials, sensitive biomarker assays of target engagement are urgently required. Our objective was to develop such an assay.MethodsWe used the single molecule array (Simoa) platform to develop an immunoassay for measuring poly(GP) dipeptide repeat proteins (DPRs) generated by theC9orf72repeat expansion in cerebrospinal fluid (CSF) of people withC9orf72-associated FTD/ALS.Results and conclusionsWe show the assay to be highly sensitive and robust, passing extensive qualification criteria including low intraplate and interplate variability, a high precision and accuracy in measuring both calibrators and samples, dilutional parallelism, tolerance to sample and standard freeze–thaw and no haemoglobin interference. We used this assay to measure poly(GP) in CSF samples collected through the Genetic FTD Initiative (N=40C9orf72and 15 controls). We found it had 100% specificity and 100% sensitivity and a large window for detecting target engagement, as theC9orf72CSF sample with the lowest poly(GP) signal had eightfold higher signal than controls and on average values fromC9orf72samples were 38-fold higher than controls, which all fell below the lower limit of quantification of the assay. These data indicate that a Simoa-based poly(GP) DPR assay is suitable for use in clinical trials to determine target engagement of therapeutics aimed at reducingC9orf72repeat-containing transcripts.
Probing the interactions between pour point depressants (PPDs), viscosity index improvers (VIIs), and wax in octane using fluorescently labeled PPDs
Authors: Gholami Kiarash , Frasca Franklin , Duhamel Jean 
Published: April 2022

A poly(octadecyl methacrylate) sample fluorescently labeled with 6.7 mol% of pyrene (Py(6.7)-PC18MA) was used as a mimic of a pour point depressant (PPD) to investigate how Py(6.7)-PC18MA interacts with wax found in engine oils and ethylene-propylene (EP) copolymers used as mimics of viscosity index improvers (VIIs). The fluorescence spectra of Py(6.7)-PC18MA solutions in octane were acquired in octane at low and high concentrations of Py(6.7)-PC18MA and analysed to obtain the molar fraction ( finter) of pyrene labels, which formed excimer intermolecularly, a measure of the level of intermolecular interactions between Py(6.7)-PC18MA molecules in the solution. The finter-versus- T profile obtained for Py(6.7)-PC18MA alone in octane confirmed that Py(6.7)-PC18MA formed microcrystals at solution temperatures below 0 °C. The effect induced by the addition of wax and an amorphous (EP(AM)) and semicrystalline (EP(SM)) EP copolymer on the interactions experienced by Py(6.7)-PC18MA were characterized by monitoring finter as a function of temperature and comparing the different finter-versus- T plots obtained after the addition of the different components with the finter-versus- T plot obtained for Py(6.7)-PC18MA alone. These studies demonstrated that wax and EP(AM) increased the level of intermolecular interactions between the Py(6.7)-PC18MA molecules at all temperatures in octane. EP(SM) increased the interactions between Py(6.7)-PC18MA molecules at high temperature, where it was soluble in octane, but finter reverted to its value in the absence of EP(SM) at low temperatures, where EP(SM) had crystallized. These experiments illustrate how pyrene excimer fluorescence can be applied to probe the complex interactions taking place between the different components found in engine oils.