bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying barriers and facilitators to psychosocial care for people living with HIV in Ireland: a mixed methods study
Authors: Burke Aoife , Davoren Martin P. , Arensman Ella , Harrington Janas 
Published: February 2025

Abstract

Background
The effectiveness of antiretroviral therapy means that human immunodeficiency virus (HIV) can now be defined as a manageable chronic illness. It is the facilitation of psychosocial care that has increasingly become a priority, as people living with HIV (PLWH) are disproportionately impacted by psychosocial stressors compared to the general population. The aim of this study was to identify barriers and facilitators to psychosocial care for PLWH in Ireland.

Methods
A mixed methods study design was used, employing a national survey of PLWH (n = 54) via Qualtrics and semi-structured interviews with healthcare professionals that provide clinical support to PLWH across Ireland (n = 11). Content analysis was used to analyse the interviews.

Results
More than half (59.3%) of survey respondents agreed that living with HIV currently impacts their mental health, with nearly two thirds (64.8%) agreeing that they have experienced stigma as a result of living with HIV. Less than half (40.7%) were comfortable disclosing their status to family, and less than a third (27.8%) to friends. Stigma was identified by healthcare professionals as a barrier to psychosocial care, along with a number of system-level barriers, provider-level or practical barriers, and individual barriers. The value of multidisciplinary care teams and reliance on community support was emphasised, and potential for further integration of support services was highlighted.

Conclusion
Community-based organisations contribute significantly to the facilitation of psychosocial support for PLWH in Ireland, and greater integration of community services could promote a more holistic, patient-centred approach to meeting the diverse needs of this growing cohort. PLWH benefit from multidisciplinary care teams, and the facilitation of safe and effective peer support should be encouraged to afford opportunities to disclose and receive social support. HIV-related stigma remains a barrier to psychosocial care, highlighting the need for stigma reduction interventions.
Development of a predictive nomogram based on preoperative inflammation-nutrition-related markers for prognosis in locally advanced lip squamous cell carcinoma after surgical treatment
Authors: Cheng Hao , Xu Xue-Lian , Zhang Zheng , Xu Jin-Hong , Li Zhuo-Ran , Wang Ya-Nan , Zhang Bo-Dong , Chen Ke , Wang Shou-Yu 
Published: February 2025

Abstract

Background
The prognostic role of preoperative inflammation-nutrition-related markers in locally advanced lip squamous cell carcinoma (LSCC) remains underexplored. This study aimed to assess the impact of various preoperative inflammation-nutrition-related markers on the prognosis of patients with locally advanced LSCC undergoing surgical treatment and to establish a corresponding predictive model.

Methods
A retrospective analysis was performed on the clinical data of 169 patients with locally advanced LSCC who underwent surgical treatment. A total of 27 clinicopathological variables, including inflammation-nutrition-related markers, were collected. Univariate and multivariate Cox regression analyses were used to identify independent prognostic factors for disease-free survival (DFS) and overall survival (OS). The nomogram models were validated using receiver operating characteristic (ROC) curve analysis, calibration plots, and decision curve analysis (DCA). Risk stratification was performed based on the nomogram scores, and differences between risk subgroups were explored.

Results
The extranodal extension (ENE), surgical safety margin, Glasgow prognostic score (GPS), Geriatric Nutritional Risk Index (GNRI), Controlling Nutrition score (CONUT), American Joint Committee on Cancer (AJCC) stage, and adjuvant radiotherapy were independent prognostic factors for DFS. In contrast, ENE, surgical safety margin, GNRI, CONUT, AJCC stage, and adjuvant radiotherapy were also independent prognostic factors for OS. The nomograms demonstrated better predictive performance than the AJCC staging system. Based on the nomogram model, patients were stratified into low-, medium-, and high-risk subgroups, which exhibited significant differences in survival outcomes.

Conclusion
GPS, GNRI, and CONUT are independent factors affecting the prognosis of patients with locally advanced LSCC undergoing radical surgery. By combining GPS, GNRI, and COUNT with other independent clinicopathological prognostic factors, a reliable nomogram model can be established to accurately predict patients' DFS and OS. This provides a powerful tool for individualized prognostic assessment, optimized risk stratification, and treatment decision-making.
Work restrictions among healthcare providers in a northern Italian public academic hospital: an observational study
Authors: Pazzaglia Silvia , Bruno Martina , Gambazza Simone , Binda Filippo , Bisesti Alberto , Calegari Jessica Graziella , Laquintana Dario 
Published: February 2025

Abstract

Background
Reduced work capacity in public hospitals has organizational repercussions, given the aging population, the shortage of healthcare workers, and the greater demand for healthcare services. In this study, we analysed the characteristics of staff assessed as "fit with restrictions" at a public academic hospital in northern Italy. We also aimed to identify individual and work-related variables that may be associated with the probability and timing of being “fit with restrictions”.

Methods
In this single-center observational study, sociodemographic data from staff employed in the Department of Healthcare Professions at our institution were analyzed using logistic regression to assess any associations between staff characteristics and the probability of being "fit with restrictions". Additionally, a multivariable Cox proportional hazard model was fitted to investigate the potential association between staff characteristics and the timing of their first assessment as "fit with restrictions".

Results
The study population was 2251 employees of which 18.4% (415/2251) were "fit with restrictions"; 56.1% (233/415) of nursing staff had at least one restriction, whereas 72.3% (300/415) of staff with restrictions had a permanent restriction. Sex was not associated with the probability of being "fit with restrictions" (odds ratio [OR] 0.75, 95%CI: 0.55 to 1.03). However, the probability was 22.9% lower (95%CI: 14.1% to 31.8%) for rehabilitation and technical healthcare staff compared to that of nurses and midwives. The Cox model showed an increase in the hazards of being "fit with restrictions" by a factor of 1.30 (95%CI: 1.02–1.68) for females.

Conclusions
A significant proportion of nursing staff face mobility and posture restrictions, with older hires and greater seniority associated with higher probabilities of restrictions. These findings underscore the importance of addressing aging and workplace conditions in the public healthcare sector, particularly considering differences across job profiles and sex.
Proceedings from the CIHLMU International Health Symposium 2023: “One Health Approach to Neglected Tropical Diseases”
Authors: Hayibor Kenneth Mawuta , Pamba Doreen Ibrahim , Banze Denise Floripes Tinga , Mfinanga Alfred Arnold , Hanago Getu Ataro , Singh Ankita , Froeschl Guenter , Hoffaeller Lisa , Scholze Sarah 
Published: February 2025

Abstract
Neglected tropical diseases (NTDs) are a group of 20 conditions that affect impoverished communities and disproportionately impact women and children in tropical areas. The symposium aimed to raise awareness of NTDs and explore the One Health approach as well as actions needed to successfully combat NTDs. It featured four presentations and two panel discussions. The presentations covered topics such as the “Burden of NTDs in Low-and middle-income countries”, “Challenges in the prevention and control of NTDs: Schistosomiasis”, “One health action needed to address NTDs: the case of Neurocysticercosis”, and “The success of one health intervention in the fight against Trachoma in Kenya”. All presenters emphasized the crucial role of the One Health integrated approach in effectively and sustainably preventing NTDs.
Injectable platelet-mimicking silk protein-peptide conjugate microspheres for hemostasis modulation and targeted treatment of internal bleeding
Authors: Shuai Yajun , Qian Yu , Zheng Meidan , Yan Chi , Wang Jue , Wang Peng , Wang Jie , Mao Chuanbin , Yang Mingying 
Published: February 2025

Abstract
Uncontrolled deep bleeding, commonly encountered in surgical procedures, combat injuries, and trauma, poses a significant threat to patient survival and recovery. The development of effective hemostatic agents capable of precisely targeting trauma sites in deep tissues and rapidly halt bleeding remains a considerable challenge. Drawing inspiration from the natural hemostatic cascade, we present platelet-like microspheres composed of silk fibroin (SF) and thrombus-targeting peptides, engineered to mimic natural platelets for rapid hemostasis in vivo. These peptide/SF hemostatic microspheres, formulated using a freezing self-assembly technology, closely resemble natural platelets in terms of size, shape, and zeta potential. Moreover, they exhibit favorable cytocompatibility, hemocompatibility, and anti-cell adhesion. Assessment of fibrin polymerization revealed that these hemostatic microspheres possessed enzymatic physiological functions, similar to activated platelets, facilitating platelet adhesion, fibrin binding, and wound-triggered hemostasis. Notably, these hemostatic microspheres rapidly target the bleeding site in vivo within 5 min, with minimal dispersion elsewhere, persisting after blood clot formation. Furthermore, these microspheres exhibit favorable metabolic kinetics, with 71% degradation occurring within one-day post-subcutaneous injection. Histological assessment revealed well-preserved organ structures and minimal inflammatory responses at 14 d post-injection, supporting their long-term biocompatibility. Importantly, they can be injected and targeted into damaged blood vessels, selectively binding to fibrin and forming blood clots within 2 min, resulting in a 74% reduction in bleeding volume compared to SF microspheres alone. Therefore, these injectable SF-based hemostatic microspheres emerge as promising candidates for future rapid hemostasis in tissue injuries.

Graphical Abstract
Proficiency in motor imagery is linked to the lateralization of focused ERD patterns and beta PDC
Authors: Angulo-Sherman Irma Nayeli , León-Domínguez Umberto , Martinez-Torteya Antonio , Fragoso-González Gilberto Andrés , Martínez-Pérez Mayté Verónica 
Published: February 2025

Abstract

Background
Motor imagery based brain-computer interfaces (MI-BCIs) are systems that detect the mental rehearsal of movement from brain activity signals (EEG) for controlling devices that can potentiate motor neurorehabilitation. Considering the problem that MI proficiency requires training and it is not always achieved, EEG desirable features should be investigated to propose indicators of successful MI training.

Methods
Nine healthy right-handed subjects trained with a MI-BCI for four sessions. In each session, EEG was recorded for 30 trials that consisted of a rest and a dominant-hand MI sequence, which were used for calibrating the system. Then, the subject participated in 160 trials in which a cursor was displaced on a screen by performing MI or relaxing to hit a target. The session’s accuracy was calculated. For each trial from the calibration phase of the first session, the power spectral density (PSD) and the partial directed coherence (PDC) of the rest and MI EEG segments were obtained to estimate the event-related synchronization changes (ERS) and the connectivity patterns of the

$$\theta$$

θ

,

$$\alpha$$

α

,

$$\beta$$

β

and

$$\gamma$$

γ

bands that are associated with high BCI control (accuracy above 70% in at least one session). Finally, t-tests and rank-sum tests (

$$p<0.05$$

p
<
0.05

, with Benjamini-Hochberg correction) were used to compare the ERS/ERD and PDC values of subjects with high and low accuracy, respectively.

Results
Proficient users showed greater

$$\alpha$$

α

ERD on the right-hand motor cortex (left hemisphere). Furthermore, the

$$\beta$$

β

PDC related to the ipsilateral motor cortex is commonly weakened during motor imagery, while the contralateral motor cortex

$$\gamma$$

γ

PDC is enhanced.

Conclusions
Motor imagery proficiency is related to the focused and lateralized event-related

$$\alpha$$

α

desynchronization patterns and the lateralization of

$$\beta$$

β

and

$$\gamma$$

γ

PDC. Future analysis of these features could allow complimenting the information for assessment of subject-specific BCI control and the prediction of the effectiveness of motor-imagery training.
Two-fold red excess (TREx): a simple and novel digital color index that enables non-invasive real-time monitoring of green-leaved as well as anthocyanin-rich crops
Authors: Agarwal Avinash , Colwell Filipe de Jesus , Galvis Viviana Andrea Correa , Hill Tom R. , Boonham Neil , Prashar Ankush 
Published: February 2025

Abstract

Background
Digital color indices provide a reliable means for assessing plant status by enabling real-time estimation of chlorophyll (Chl) content, and are thus adopted widely for crop monitoring. However, as all prevalent leaf color indices used for this purpose have been developed using green-leaved plants, they do not perform reliably for anthocyanin (Anth)-rich red-leaved varieties. Hence, the present study investigates digital color indices for six types of leafy vegetables with different levels of Anth to identify congruent trends that could be implemented universally for non-invasive crop monitoring irrespective of species and leaf Anth content. For this, datasets from three digital color spaces, viz., RGB (Red, Green, Blue), HSV (Hue, Saturation, Value), and L*a*b* (Lightness, Redness-greenness, Yellowness-blueness), as well as various derived plant color indices were compared with Anth/Chl ratio and SPAD Chl meter readings of n = 320 leaf samples.

Results
Logarithmic decline of G/R, G-minus-R, and Augmented Green-Red Index (AGRI) with increasing Anth/Chl ratio (R

2
> 0.8) revealed that relative Anth content affected digital color profile markedly by shifting the greenness-redness balance until the Anth/Chl ratio reached a certain threshold. Further, while most digital color features and indices presented abrupt shifts between Anth-rich and green-leaved samples, the proposed color index Two-fold Red Excess (TREx) did not exhibit any deviation due to leaf Anth content and showed better correlation with SPAD readings (R

2
= 0.855) than all other color features and vegetation indices.

Conclusion
The present study provides the first in-depth assessment of variations in RGB-based digital color indices due to high leaf Anth contents, and uses the data for Anth-rich as well as green-leaved crops belonging to different species to formulate a universal digital color index TREx that can be used as a reliable alternative to handheld Chl meters for rapid high-throughput monitoring of green-leaved as well as red-leaved crops.
Production of HSVd- and PPV-free apricot cultivars by in vitro thermotherapy followed by meristem culture
Authors: Pérez-Caselles C. , Burgos L. , Yelo E. , Faize L. , Alburquerque N. 
Published: February 2025

Abstract

Background
The production of virus-free apricots (Prunus armeniaca L.) is essential for controlling viral diseases, exchanging breeding materials without the risk of spreading new diseases, and preserving plant germplasm. Plum pox virus (PPV) is the most devastating disease of the Prunus genus and Hop stunt viroid (HSVd) is prevalent in most apricot-growing regions. It was evaluated whether thermotherapy, etiolation, or a combination of both followed by meristem culture could effectively eliminate PPV and HSVd from ‘Canino’ and ‘Mirlo Rojo’ apricot cultivars in vitro.

Results
In the thermotherapy treatments, shoots were exposed to 38ºC and 32ºC, alternating every four hours, for 30, 35, 40, and 45 days. Before this, shoots were acclimated to heat for one day at 28ºC and two days at 30ºC. Etiolation experiments consisted of eight weeks of culture in dark conditions. A combination of 45 days of thermotherapy, as described previously, and etiolation was also performed. At the end of each treatment, 1.5 mm meristems were cultured, and developed as potential independent pathogen-free lines. The presence or absence of pathogens was analysed by RT-PCR. The 45 days of thermotherapy and the combined thermotherapy and etiolation treatments resulted in the highest percentages of PPV-free plants (66.7 and 75.0%, respectively). At least 40 days of thermotherapy were required to obtain HSVd-free plants, although the best efficiency was achieved at 45 days (22.7%).

Conclusions
In this study, we have developed an effective in vitro thermotherapy protocol that eliminates PPV and HSVd from apricot cultivars. This is the first report where a thermotherapy protocol eliminates HSVd in Prunus species.
Impact of enzyme replacement therapy and migalastat on disease progression in females with fabry disease
Authors: Lenders Malte , Nowak Albina , Cybulla Markus , Kaufeld Jessica , Köhn Anja Friederike , Muschol Nicole Maria , Kurschat Christine , Brand Eva 
Published: February 2025

Abstract

Aim
The aim of our multicenter study was to investigate the safety and efficacy of enzyme replacement therapy (ERT) and chaperone therapy on the disease progression in female Fabry disease (FD) patients and to compare the individual treatment regimens.

Methods
Data from 3 consecutive visits of 102 female FD patients from 6 Fabry centers were retrospectively analyzed. According to their FD-specific treatment, patients were separated in 5 groups: Newly agalsidase-beta- [n = 18], agalsidase-alfa- [n = 29] and migalastat-[n = 14] treated patients, and long-term agalsidase-beta- [n = 7] and agalsidase-alfa-[n = 34] treated patients. Clinical presentation and laboratory data, including plasma lyso-Gb3 levels were assessed.

Results
Treatment with agalsidase-beta, agalsidase-alfa, and migalastat was safe and severe adverse events were rare. Newly and long-term-treated patients presented a stable disease course over time. None of the patients required hospitalization due to cardiac events. Overall septum thickness remained stable in all groups (p > 0.05). Estimated glomerular filtration rate (eGFR) only slightly decreased in patients treated with agalsidase-alfa [newly- and long-term-treated: -1.5 ± 3.2 and − 1.3 ± 3.9 ml/min/1.73 m²/year; p = 0.0056 and p = 0.0187, respectively] but the decrease was in the range of natural eGFR decline. eGFRs in agalsidase-beta and migalastat-treated patients were stable. No clinically relevant differences concerning treatment efficacy between agalasidase-beta, agalsidase-alfa, and migalastat were detected.

Conclusion
We conclude that treatment of females with agalsidase-beta, agalsidase-alfa, and migalastat was safe. Independent of the chosen treatment regimen, nearly all patients presented with a stable disease course over time. In our cohort, a comparison of therapy efficacies showed no relevant clinical differences between the groups.
CDCA3-MYC positive feedback loop promotes bladder cancer progression via ENO1-mediated glycolysis
Authors: Shen Dexin , Yu Xiang , Fan Xuefeng , Liang Yu , Lu Dongmei , Ke Zongpan , Wang Lei , Xiang Ping , Xiao Jun 
Published: February 2025

Abstract

Background
Bladder cancer (BLCA) ranks among the most prevalent malignancies of the urinary system, with its clinical diagnosis predominantly reliant on invasive procedures. Traditional chemotherapy regimens exhibit significant limitations, underscoring the urgency of identifying novel diagnostic biomarkers and strategies to enhance chemotherapy efficacy. CDCA3 has been recognized as a facilitator of BLCA progression, activated by MYBL2. However, its precise regulatory mechanisms in BLCA pathogenesis remain incompletely elucidated.

Methods
To investigate the functional role of CDCA3 in BLCA, MTT and colony formation assays were employed to assess cellular proliferation, while flow cytometry was utilized to evaluate apoptosis and intracellular ROS levels. The expression of CDCA3, ENO1, TRIM28, and MYC was analyzed through WB and qRT-PCR, and Co-IP assays were conducted to delineate interactions among CDCA3, TRIM28, and MYC.

Results
CDCA3, a key regulator of the cell cycle, facilitates BLCA glycolysis by modulating the transcriptional expression of α-Enolase (ENO1), thereby enhancing BLCA progression. Mechanistically, CDCA3 recruits TRIM28, which stabilizes MYC, while MYC transcriptionally upregulates CDCA3, establishing a self-reinforcing CDCA3-MYC feedback loop. A risk prediction model incorporating the expression profiles of CDCA3 and ENO1 was developed to evaluate the overall survival of patients with BLCA. This model provides a prognostic tool to predict survival outcomes in patients with BLCA based on CDCA3 and ENO1 expression levels.

Conclusions
This study delineates a novel role for CDCA3 in the regulation of BLCA glycolysis and identifies its interaction with MYC as a critical positive feedback mechanism, providing fresh insights into the molecular mechanisms underlying BLCA progression.
Critical interventions, diagnosis, and mortality in children treated by a physician-manned mobile emergency care unit
Authors: McKenzie Alexandra Claire , Risom Mads Belger , Møller Jens-Jakob Kjer , Mikkelsen Johan , Skole-Sørensen Sarah Friis , Nielsen Vibe Maria Laden , Clausen Nicola Groes , Mikkelsen Søren 
Published: February 2025

Abstract

Background
The purpose of this study was to clarify the potentially life-saving critical interventions performed on children below the age of seven by the physician-manned mobile emergency care unit (MECU) in Odense, Denmark. We investigated critical interventions in relation to morbidity and mortality.

Methods
A retrospective cohort study of all MECU missions involving children below the age of seven. The study period was from October 1 2007 to December 31 2020. Data sources were the MECU Odense database, the Danish National Patient Registry, and the Danish Civil Registration System. Variables were critical interventions, the severity of injury/illness, MECU on-scene time, in-hospital diagnosis and 7-day, 30-day, and 90-day mortality.

Results
The MECU carried out 4,032 missions to children below 7 years. 88 patients (2.2%) received at least one critical prehospital intervention. Upper airway suction was performed in 39 cases (1.0%), endotracheal intubation (all causes) in 36 cases (0.9%), and intraosseous access in 21 cases (0.5%). General anaesthesia was induced in 29 cases (0.7%). Seventeen patients (0.4%) received cardiopulmonary resuscitation and two patients received manual defibrillation (< 0.1%). 3,278 patients were admitted to the hospital and assigned a diagnosis when discharged. The most common diagnoses were assigned within the International Statistical Classification of Diseases and Related Health Problems 10th Revision Chapter XVIII (Symptoms, signs and abnormal clinical and laboratory findings, not elsewhere classified), which includes febrile convulsions. 1,437 patients (43.8%) were assigned diagnoses within this diagnosis group.
The overall 7-day mortality in the cohort was 0.74%, 30-day mortality was 0.82%, and 90-day mortality was 1.02%.

Conclusion
Prehospital critical interventions are rarely performed in children under the age of 7 years. The low frequency of these interventions may have implications for maintaining the clinical routine of the prehospital care providers.