bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fibromyalgia patients with high levels of anti-satellite glia cell IgG antibodies present with more severe symptoms
Authors: Krock Emerson , Morado-Urbina Carlos E. , Menezes Joana , Hunt Matthew A. , Sandström Angelica , Kadetoff Diana , Tour Jeanette , Verma Vivek , Kultima Kim , Haglund Lisbet , Meloto Carolina B. , Diatchenko Luda , Kosek Eva , Svensson Camilla I. 
Published: July 2022

ObjectiveTransferring fibromyalgia patient IgG to mice induces pain-like behaviour and fibromyalgia IgG binds mouse and human satellite glia cells (SGCs). These findings suggest that autoantibodies could be part of fibromyalgia pathology. However, it is unknown how frequently fibromyalgia patients have anti-SGC antibodies and how anti-SGC antibodies associate with disease severity.MethodsWe quantified serum or plasma anti-SGC IgG levels in two fibromyalgia cohorts from Sweden and Canada using an indirect immunofluorescence murine cell culture assay. Fibromyalgia serum IgG binding to human SGCs in human dorsal root ganglia tissue sections was assessed by immunofluorescence (n=14/group).ResultsIn the cell culture assay anti-SGC IgG levels were increased in both fibromyalgia cohorts compared to controls. Elevated anti-SGC IgG was associated with higher levels of self-reported pain in both cohorts, and higher fibromyalgia impact questionnaire scores and increased pressure sensitivity in the Swedish cohort. Anti-SGC IgG levels were not associated with fibromyalgia duration. Swedish FM patients were clustered into FM-severe and FM-mild groups and the FM-severe group had elevated anti-SGC IgG compared to the FM-mild and controls. Anti-SGC IgG levels detected in culture were positively correlated with increased binding to human SGCs. Moreover, the FM-severe group had elevated IgG binding to human SGCs compared to the FM-mild and control groups.ConclusionsA subset of fibromyalgia patients have elevated levels of anti-SGC antibodies, and the antibodies are associated with more severe fibromyalgia severity. Screening fibromyalgia patients for anti-SGC antibodies could provide a path to personalized treatment options that target autoantibodies and autoantibody production.
An Early Miocene skeleton ofBrachydiceratheriumLavocat, 1951 (Mammalia, Perissodactyla) from the Baikal area, Russia, and a revised phylogeny of Eurasian teleoceratines
Authors: Sizov Alexander , Klementiev Alexey , Antoine Pierre-Olivier 
Published: July 2022

AbstractHippo-like rhinocerotids, or teleoceratines, were a conspicuous component of Holarctic Miocene mammalian faunas, but their phylogenetic relationships remain poorly known. Excavations in lower Miocene deposits of the Olkhon Island (Tagay locality, Eastern Siberia; 16–18 Ma) have opened a unique window on the poorly-known early history of the Lake Baikal ecosystems, notably by unearthing a skeleton of the teleoceratineBrachydiceratherium shanwangense(Wang, 1965). The remains provide new insights into the skull and postcranial morphology of this elusive species. The new material is compared with other Eurasian teleoceratines and the relationships within Teleoceratina are investigated through a phylogenetic analysis.DiaceratheriumDietrich, 1931 (earliest Miocene, Western Europe) is found to be monotypic and is retrieved as the earliest teleoceratine offshoot. Other genera have more than one species and are also found to be monophyletic, withProsantorhinusHeissig, 1974 (early Miocene, Eurasia) +TeleocerasHatcher, 1894 (Miocene, North America) forming the sister clade ofBrachypotheriumRoger, 1904 (Miocene, Old World) +BrachydiceratheriumLavocat, 1951.Brachydiceratheriumincludes eight species spanning the late Oligocene to Late Miocene in Europe and Asia. All teleoceratine genera exceptDiaceratheriumspan considerable geographical and stratigraphical ranges, likely related to their ultra-generalist ecological preferences.
Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction
Authors: Yeh Yu-Te , Sona Chandan , Yan Xin , Pathak Adrija , McDermott Mark I. , Xie Zhigang , Liu Liangwen , Arunagiri Anoop , Wang Yuting , Cazenave-Gassiot Amaury , Ghosh Adhideb , von Meyenn Ferdinand , Kumarasamy Sivarajan , Najjar Sonia M. , Jia Shiqi , Wenk Markus R. , Traynor-Kaplan Alexis , Arvan Peter , Barg Sebastian , Bankaitis Vytas A. , Poy Matthew N. 
Published: July 2022

SUMMARYDefects in insulin processing and granule maturation are linked to pancreatic beta-cell failure during type 2 diabetes (T2D). Phosphatidylinositol transfer protein alpha (PITPNA) stimulates activity of phosphatidylinositol (PtdIns) 4-OH kinase to produce sufficient PtdIns-4-phosphate (PtdIns-4-P) in the trans-Golgi network to promote insulin granule maturation. PITPNA in beta-cells of T2D human subjects is markedly reduced suggesting its depletion accompanies beta-cell dysfunction. Conditional deletion of Pitpna in the beta-cells of Ins-Cre;Pitpnaflox/flox mice leads to hyperglycemia resulting from decreased glucose-stimulated insulin secretion (GSIS) and reduced pancreatic beta-cell mass. Furthermore, PITPNA silencing in human islets confirmed its role in PtdIns-4-P synthesis and led to impaired insulin granule maturation and docking, GSIS, and proinsulin processing with evidence of ER stress. Restoration of PITPNA in islets of T2D human subjects reversed these beta-cell defects and identify PITPNA as a critical target linked to beta-cell failure in T2D.
NAD+repletion with niacin counteracts cancer cachexia
Authors: Beltrà Marc , Pöllänen Noora , Fornelli Claudia , Tonttila Kialiina , Hsu Myriam Y. , Zampieri Sandra , Moletta Lucia , Porporato Paolo E. , Kivelä Riikka , Sandri Marco , Hulmi Juha J. , Sartori Roberta , Pirinen Eija , Penna Fabio 
Published: July 2022

SUMMARYCachexia is a debilitating wasting syndrome and highly prevalent comorbidity in cancer patients. It manifests especially with energy and mitochondrial metabolism aberrations that promote tissue wasting. We recently identified nicotinamide adenine dinucleotide (NAD+) loss to associate with muscle mitochondrial dysfunction in cancer hosts. In this study we confirmed that depletion of NAD+and downregulation ofNrk2, an NAD+biosynthetic enzyme, are common features of different mouse models and cachectic cancer patients. Testing NAD+repletion therapy in cachectic mice revealed that NAD+precursor, vitamin B3 niacin, efficiently corrected tissue NAD+levels, improved mitochondrial metabolism and ameliorated cancer- and chemotherapy-induced cachexia. To examine NAD+metabolism in a clinical setting, we showed that the low expression ofNRK2in cancer patients correlated with metabolic abnormalities underscoring the significance of NAD+in the pathophysiology of human cancer cachexia. Overall, our results propose a novel therapy target, NAD+metabolism, for cachectic cancer patients.
A Dimer between Monomers and Hexamers – Oligomeric Variations in Glucosamine 6-Phosphate Deaminase Family
Authors: Srinivasachari Sathya , Tiwari Vikas R. , Kharbanda Tripti R. , Sowdamini Ramanathan , Subramanian Ramaswamy 
Published: July 2022

ABSTRACTIn bacteria that live in hosts whose terminal sugar is a sialic acid, Glucosamine 6-phosphate deaminase (NagB) catalyzes the last step in the conversion of sialic acid into Fructose-6-phosphate, which enters the glycolytic pathway. The enzyme exists as a hexamer in Gram-negative bacteria and is shown to be allosterically regulated. In Gram-positive bacteria, it exists as a monomer and lacks allosteric regulation. Our identification of a dimeric Gram-negative bacterial NagB motivated us to characterize the structural basis of the various oligomeric forms. We characterized the crystal structures of NagB from two Gram-negative pathogens, Haemophilus influenzae (Hi) and Pasturella multocida (Pm). The Hi-NagB is active as a hexamer, while Pm-NagB is active as a dimer. We confirm that this is not a crystallographic artifact by cryo-electron microscopy. Both Hi-NagB and Pm-NagB contain the C-terminal helix, and the residues in the interface involved in oligomerization are conserved. The hexamer is described as a dimer of trimers. In the Pm-NagB dimer, the dimeric interface is conserved. This would suggest that the three possible oligomeric forms of NagB are a monomer, a dimer, and a trimer of dimers. Computational modeling and MD simulations indicate that the residues at the trimeric interface have less stabilizing energy of oligomer formation than those in the dimer interface. We propose that Pm-NagB is the evolutionary link between the monomer and the hexamer forms.
An interpretable machine learning algorithm to predict disordered protein phase separation based on biophysical interactions
Authors: Cai Hao , Vernon Robert M. , Forman-Kay Julie D. 
Published: July 2022

AbstractProtein phase separation is increasingly understood to be an important mechanism of biological organization and biomaterial formation. Intrinsically disordered protein regions (IDRs) are often significant drivers of protein phase separation. A number of protein phase separation prediction algorithms are available, with many specific for particular classes of proteins and others providing results that are not amenable to interpretation of contributing biophysical interactions. Here we describe LLPhyScore, a new predictor of IDR-driven phase separation, based on a broad set of physical interactions or features. LLPhyScore uses sequence-based statistics from the RCSB PDB database of folded structures for these interactions, and is trained on a manually curated set of phase separation driver proteins with different negative training sets including the PDB and human proteome. Competitive training for a variety of physical chemical interactions shows the greatest importance of solvent contacts, disorder, hydrogen bonds, pi-pi contacts, and kinked-beta structure, with electrostatics, cation-pi, and absence of helical secondary structure also contributing. LLPhyScore has strong phase separation prediction recall statistics and enables a quantitative breakdown of the contribution from each physical feature to a sequence’s phase separation propensity. The tool should be a valuable resource for guiding experiment and providing hypotheses for protein function in normal and pathological states, as well as for understanding how specificity emerges in defining individual biomolecular condensates.Graphical Abstract
Improving Bacterial Genome Assembly Using a Test of Strand Orientation
Authors: Greenberg Grant , Shomorony Ilan 
Published: July 2022

AbstractThe complexity of genome assembly is due in large part to the presence of repeats. In particular, large reverse-complemented repeats can lead to incorrect inversions of large segments of the genome. To detect and correct such inversions in finished bacterial genomes, we propose a statistical test based on tetranucleotide frequency (TNF), which determines whether two segments from the same genome are of the same or opposite orientation. In most cases, the test neatly partitions the genome into two segments of roughly equal length with seemingly opposite orientations. This corresponds to the segments between the DNA replication origin and terminus, which were previously known to have distinct nucleotide compositions. We show that, in several cases where this balanced partition is not observed, the test identifies a potential inverted misassembly, which is validated by the presence of a reverse-complemented repeat at the boundaries of the inversion. After inverting the sequence between the repeat, the balance of the misassembled genome is restored. Our method identifies 31 potential misassemblies in the NCBI database, several of which are further supported by a reassembly of the read data.
The Gut Resistome during Hematopoietic Stem Cell Transplantation in Children
Authors: Heston Sarah M. , Young Rebecca R. , Jenkins Kirsten , Martin Paul L. , Stokhuyzen Andre , Ward Doyle V. , Bhattarai Shakti K. , Bucci Vanni , Arshad Mehreen , Chao Nelson J. , Seed Patrick C. , Kelly Matthew S. 
Published: July 2022

AbstractBackgroundChildren undergoing hematopoietic stem cell transplantation (HCT) are at high risk of acquiring antibiotic-resistant bacteria. Few prior studies examined antibiotic resistance genes (ARGs) within the gut metagenomes of children undergoing HCT.MethodsWe conducted a longitudinal study of children (age <18 years) undergoing HCT at a single institution. We performed shotgun metagenomic sequencing of fecal samples collected between days -30 and +100 relative to HCT. We evaluated the effects of aerobic (cefepime, vancomycin, fluoroquinolones, aminoglycosides, macrolides, and trimethoprim-sulfamethoxazole) and anaerobic (piperacillin-tazobactam, carbapenems, metronidazole, and clindamycin) antibiotic exposures on the diversity and composition of the gut microbiome and resistome.FindingsUsing metagenomic data from 693 fecal samples collected from 80 children, we identified 350 unique ARGs. The most frequent ARGs identified encode resistance to tetracycline (n=91), beta-lactams (n=80), and fluoroquinolones (n=76). Both aerobic and anaerobic antibiotic exposures were associated with a decrease in the number of bacterial species (aerobic, β=0.72, 95% CI: 0.66, 0.79; anaerobic, β=0.68, 95% CI: 0.61, 0.76) and the number of unique ARGs (aerobic, β=0.83, 95% CI: 0.76, 0.91; anaerobic, β=0.84, 95% CI: 0.76, 0.93) within the gut metagenome. However, only anaerobic antibiotics were associated with an increase in the number of newly acquired ARGs (29%, 95% CI: 10%, 52%) and the abundance of ARGs (95%, 95% CI: 59%, 138%) in the gut resistome. Specific antibiotic exposures were associated with distinct changes in the number and abundance of resistance genes for individual antibiotic classes.InterpretationThe gut metagenome and resistome of children are highly dynamic throughout HCT, driven largely by antibiotic exposures. Compared to antibiotics without anaerobic activity, anaerobic antibiotics were associated with increased microbiome instability and expansion of the gut resistome.FundingAntibacterial Resistance Leadership Group, National Institutes of Health, Duke Children’s Health & Discovery Initiative, Children’s Miracle Network Hospitals
Consumers' cultural identity under glocalization: Vietnamese consumers' global and national identities and their cross-cultural consumption
Authors: Choo Ho Jung , Lee Ha Kyung , Xie Jiali 
Published: July 2022

PurposeThis study aims to investigate the influences of two facets of Vietnamese consumers' cultural identities (i.e. global and national) on their intent to consume Korean lifestyle products and services via attitudes toward Korea. The difference between generations (Generation Z vs. X) is examined.Design/methodology/approachData are collected through an online survey firm. The participants are Vietnamese consumers residing in Vietnam, varying in age from teens to those in their 50s (n = 500). The collected data are analyzed by SPSS 21.0 for the descriptive statistics, frequency analysis, and reliability analysis. AMOS 21.0 is employed for confirmatory factor analysis and structural equation modeling (SEM) analysis.FindingsThis study reveals that global identity affects Vietnamese consumers' attitudes toward Korea and their intent to consume Korean lifestyle products and services. Results show that only global identity affects attitudes and behavioral intention toward Korea among generation Xers, while national identity has no effect. For Generation Z (Gen Z), both global and national identities have a positive effect on attitudes toward Korea, which also increases the intent to consume Korean lifestyle products and services.Practical implicationsMeasuring individuals' global and national identities will allow brands and retailers to better understand international consumers of various generations and develop global marketing strategies.Originality/valueThis study bridges gaps in the literature on globalized consumption in a non-Western context by identifying how consumers in emerging markets become involved in cross-cultural consumption.
Board Characteristics and performance of listed firms in Ghana
Authors: Andoh Jennifer A.N. , Abugri Benjamin A. , Anarfo Ebenezer B. 
Published: July 2022

PurposeThis study aims to compare the impact of board characteristics on the performance of listed non-financial firms to the impact of board characteristics on the performance of listed financial firms (commercial banks) in Ghana.Design/methodology/approachThe fixed and random effects models with generalized least square specifications are used in estimating regressions to correct for heteroscedasticity and serial correlation. Additionally, this study uses lagged models of the board variables to address the possibility of the presence of endogeneity and to generate robust estimates.FindingsThe empirical results show some similarities and differences on the impact of board characteristics on the performance of listed non-financial firms and banks. On similarities, for both non-financial firms and banks, board size is seen to have a significant non-linear impact on Tobin’sq. Also, the proportion of foreign board members shows a positively significant relationship with firm performance for both listed non-financial firms and banks. The effect of the proportion of board members with higher educational qualifications on firm performance appears to be negative and statistically significant for both sample of firms. On the other hand, the impact of board composition and board gender diversity on firm performance differs from listed banks and non-financial firms.Research limitations/implicationsThe panel regressions for the listed banks were run on 63 observations because of the small sample size for the listed banks. Though enough for estimation purposes, inferences from results should be made with caution.Originality/valueThis paper, unlike most corporate governance – firm performance studies, focuses not only on listed non-financial firms but also on listed banks. From a multi-theoretical perspective, this paper provides a comparative analysis on the impact of board characteristics on financial performance of listed non-financial firms and banks.
Organizational learning as the best business practice for adaptation in times of great changes: a viewpoint
Authors: Csath Magdolna 
Published: July 2022

Purpose
This article summarizes survey findings from a recent study that examined how Hungarian companies of different sizes from different business fields responded to the unexpected, disruptive changes caused by the pandemic, and whether they used organizational learning to design a more adaptive and resilience enhancing business model.

Methodology
The survey was conducted via questionnaires and sent out to a selected group of business leaders of larger enterprises in receipt of state financial support. The survey focused on finding out whether using the fund was accompanied by internal learning. Interviews were also held with small businesses from a randomly selected sample to evaluate the difference in business behavior in companies of different sizes.

Findings
The first observation was that it is the value system, personal goals and level of future orientation of the top leaders, rather than the size or field of activity of the business, that determines whether organizational learning is a general practice. Second, smaller businesses may be unable to define “organization learning,” but they are more agile and forward looking. Third, in the sample of larger businesses it was more typical to find top-down decision-making and less interest in creating a learning culture.

Originality
This survey covered different sized businesses, using both questionnaire and interview-based information gathering, about the impacts of the pandemic on organizational learning practices. It offers original observations that may be interesting for researchers of the field, and also for practitioners in any country.
A facilities management approach to rationalising occupants’ energy behaviours
Authors: Weerasinghe Achini Shanika , Rasheed Eziaku Onyeizu , Rotimi James Olabode Bamidele 
Published: July 2022

Purpose
This paper aims to investigate building managers’ approach towards occupant energy behaviours and rationalises organisational energy culture concerning their strategy to address occupants’ preferences in New Zealand tertiary office buildings.

Design/methodology/approach
The research used grounded theory analysis by interviewing a purposive sample of 25 participants from a university. Also, semi-structured interviews were conducted with facilities managers, sustainability managers and building occupants.

Findings
The study results revealed that building managers oversimplify the multi-domain discomfort, energy impacts from occupant behaviours and the influence of social-psychology aspects on occupants’ actions. The organisational energy culture can be further improved by increasing occupants’ knowledge and awareness of energy, sharing energy feedback with occupants to make energy conscious occupants and giving them responsibilities to achieve the organisation’s energy targets.

Originality/value
This study enables opportunities to promote collaboration between building managers and occupants by comparing perspectives on occupant energy impacts.
How gender moderates the mediating mechanism across social experience, self-referent beliefs and social entrepreneurship intentions
Authors: Ko Eun-Jeong , Kang Jiyun 
Published: July 2022

PurposeThis study aims to understand how social experience influences social entrepreneurial (SE) intentions through different types of self-referent beliefs and how gender affects this mechanism.Design/methodology/approachTo test this study’s conceptual model, the authors conducted an online survey and recruited respondents via Amazon’s Mechanical Turk. This study’s analysis is based on 743 responses. This study used structural equation modeling to test the main hypotheses, conducted decomposition tests using the bootstrapping method to test mediation effects via self-referent beliefs and executed multi-group analyses to examine gender-moderated mediation effects.FindingsThe results confirm that social experience significantly influences all three types of self-referent beliefs (entrepreneurial self-efficacy, SE self-efficacy and self-esteem). Furthermore, the mediating relationship across social experience, self-efficacies, and SE intentions is moderated by gender, as the relationships between social experience and self-efficacies are stronger for women than for men.Originality/valueA clear gender gap exists in the way how social experience affects perceptual variables (self-referent beliefs), providing a practical suggestion to reduce the perceptual gender gap in social entrepreneurial contexts. This study also reveals the mediating mechanism across social experience, self-efficacies and SE intentions, also highlighting the importance of domain specific self-efficacies. This study’s findings support and extend Milliken’s (1987) framing of three distinct types of uncertainty to explain how individuals form SE intentions.