bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative proteomic analysis to understand the role of Arabidopsis thaliana LEAFY COTYLEDON 2 promoting lipid accumulation in Chlorella sorokiniana by upregulating photosynthetic proteins and G3PDH
Authors: Liu Changbin , Liu Yilin , Yu Shanshan , Dong Wenhao , Xue Yongchang 
Published: April 2022

Abstract
Microalgae attracted significant attention towards biofuel production and transcription factors that can effectively regulate lipid accumulation in higher plants. In this study, we expressed the transcription factor LEAFY COTYLEDON 2 from Arabidopsis thaliana on Chlorella sorokiniana. The electron transport rate (ETR) of C. sorokiniana-AtLEC2 improved significantly under high-intensity light. As a result, the total lipid content and main fatty acid composition (C16, C18) increased by more than one fold. Differentially expressed proteins were analyzed through a label-free strategy to elucidate the regulation mechanism of AtLEC2. The results showed upregulation of TAG biosynthesis (G3PDH) and photosynthesis-related proteins (e.g., ATP synthase F0, Photosystem II, Photosystem I P700, Cytochrome b6, Cytochrome b6-f, Chlorophyll a-b binding protein, Photosystem I assembly protein). Furthermore, AtLEC2 expression in C. sorokiniana led to fixed carbon flow in photosynthesis to TAG through G3PDH upregulation. The current study will provide a new direction towards cultivating high-yield oil algae and reveal the regulation mechanism of AtLEC2 on TAG accumulation in C. sorokiniana.
Intraocular Lens Placement in the Absence of Capsular Support: Visual Outcomes and Complications in a Philippine Tertiary Hospital
Authors: Torrefranca Aramis B. , Cariño Noel S. 
Published: April 2022

Abstract
Purpose:Surgical correction of aphakia without capsular support continues to be a challenge. Rapid rise in technology has provided advancements in techniques in surgical management of aphakia. Locally, we have limited data on the outcomes of the different techniques. This study aimed to determine visual outcomes and complications associated with different techniques of intraocular lens implantation in the absence of capsular support. Setting: The study was conducted at the Philippine General Hospital in Manila, Philippines.Design: This was a retrospective chart review study in a single center.Methods:We reviewed the medical charts of 207 patients who underwent intraocular lens implantation without capsular support. Excluded were patients with incomplete follow up, pediatric patients, and lost records. Best corrected visual acuity at day 1, 1st month, 3rd month and 6th month postoperatively, and the complications were noted. Results:Mean age was 60 with 51% (n=105) were females. Loss of capsular support was most frequently caused by intraoperative complication (n=146, 70%) and trauma. Retropupillary fixation iris claw intraocular lens was frequently used (n=93, 44.9%). Across all patients, visual acuities showed excellent outcomes with 20/50 or better. The presence of dense cataracts (p=0.0088) was associated with postoperative complications. Across IOL types, the most frequent postoperative complication was increase in IOP. Statistically significant results were set at P <0.05.Conclusion:Intraocular lenses used in aphakia showed good postoperative visual acuities. Complications include elevated intraocular pressures, corneal edema, and pigment dispersion. Knowledge on the outcomes maximizes the chances of optimal surgical and visual outcomes.
Outcomes of Sliding Hip Screw Fixation in Stable versus Unstable Intertrochanteric Hip Fractures
Authors: Assad Waleed , Younmis Manaf , Abuodeh Yousef , Shiraz Salman , Ahmed Ghalib 
Published: April 2022

Abstract
Background: Unstable intertrochanteric (IT) fractures are common orthopedic injuries in elderly with high morbidity. The treatment of unstable IT fractures with Sliding Hip Screw (SHS) is still controversial. The purpose of our study is to assess the outcomes of conventional SHS in unstable IT fractures in comparison to stable ones.Methods: Thirty-five stable and 23 unstable IT fractures retrospective cases treated with SHS between January 2017 and December 2018. Patients were evaluated for infection, revision rate, screw cut-out, the development of post-traumatic arthritis or heterotopic ossification and mortality. Results: Mean age of the patients at the time of surgery was 69.8 and 73.9 years for stable and unstable IT fractures, retrospectively, with 38 males and 20 females. The mean follow-up of 45.9 weeks. We found significant association between occurrence of SHS screw cutout and unstable fractures compared to stable ones (0% versus 17.4%, respectively, p = 0.01). There was an obvious trend for non-union and higher revision rate in unstable IT fractures (17.4% vs. 2.9%, p = 0.056). Logistic regression analysis didn’t reveal any statistically significant confounder that might affect the rates of fracture union or revision rates. Post-traumatic arthritis and heterotopic ossification occurred in one case of unstable IT fracture. There were no reported cases of infection.Conclusion: There is high risk of screw cutout, nonunion and revision using the SHS in unstable intertrochanteric fractures (AO type A2.2 and A2.3) compared to stable ones (AO type A2.1). Therefore, other modalities are preferred for the fixation of unstable IT fractures. Level of Evidence: III
Calculation of the Passive Earth Pressure of the Finite Soil behind Retaining Walls Based on Simplified Layering
Authors: Wang Weiwei , Liu Xinxi , Li Bin , Hu Weidong , Luo Hua 
Published: April 2022

Abstract
To investigate the passive earth pressure of finite soil behind retaining walls, this study considered the principal stress deflection caused by the arching effect of soils. To this end, the shape of the principal stress trajectory was simplified to a straight line, and the finite soil behind retaining walls was divided into several thin-layer elements along the simplified straight line. Subsequently, the calculation formulas for the passive earth pressure distribution, resultant force, and height of its action point were obtained based on the limit equilibrium condition of the thin-layer elements. In addition, the correctness and rationality of the proposed method were verified by comparing its values to those of previously reported experiments and theories. The parameter analysis revealed that the resultant force of passive earth pressure decreased significantly at the initial stage with an increase in the aspect ratio of the finite soil and then remained constant, whereas the height of the action point of resultant force increased gradually and then remains unchanged.
Combined effect of microbially-derived caecal SCFA and host genetics on feed efficiency in broiler chickens
Authors: He Zhengxiao , Liu Ranran , Wang Qiao , Zheng Jumei , Ding Jiqiang , Wen Jie , Fahey Alan G , Zhao Guiping 
Published: April 2022

Abstract
Background
Improving feed efficiency is the most important goal for modern animal production. The regulatory mechanisms of controlling feed efficiency traits are extremely complex and include the functions related to host genetics and gut microbiota. Short-chain fatty acids (SCFAs), as significant metabolites of microbiota, could be used to refine the combined effect of host genetics and gut microbiota. However, the association of SCFAs with the gut microbiota and host genetics for regulating feed efficiency is far from understood.
Results
In this study, we examined host genome sequence, microbial data and SCFA concentration of caecal chyme in 300 broilers. The SNP-based heritability analysis found that the SCFA concentrations had moderate to high heritability (h2 = 0.183 ~ 0.401). Genome-wide association studies (GWAS) showed that four out of seven SCFAs had significant associations with genome variants. One locus (gga4: 29414391–29417189) was significantly associated with propionate, locating near or inside the genes MAML3, SETD7 and MGST2, and had a modest effect on feed efficiency traits and the microbiota. The genetic effect of the top SNP explained 8.43% phenotype of propionate. Individuals with genotype AA had significantly different propionate concentrations (0.074 vs. 0.131 ug/mg), feed efficiency (FCR: 1.658 vs. 1.685), and relative abundance of 14 taxa compared to those with the GG genotype. Christensenellaceae and Christensenellaceae_R-7_group were identified being associated with feed efficiency, propionate concentration and top SNP genotypes, and they were found to be lipid metabolism-related. Individuals with a higher caecal abundance of these taxa showed better feed efficiency and lower concentrations of caecal SCFAs.
Conclusion
Our study concluded that host genetic variation could affect the regulation of caecal microbially-derived SCFAs, which plays a role in host feed efficiency traits. The caecal taxa Christensenellaceae and Christensenellaceae_R-7_group were identified as representative taxa contributing to the combined effect of host genetics and SCFAs on chicken feed efficiency. These findings proved strong evidence of the combined effect of host genetics and gut microbial SCFAs in regulating feed efficiency traits.
CHRDL2 promotes cell proliferation by activating the YAP/TAZ signaling pathway in gastric cancer
Authors: Wang Lingquan , Xu Wei , Mei Yu , Wang Xufeng , Liu Wentao , Yan Chao , Li Chen , Zhu Zhenggang , Ni Zhentian 
Published: April 2022

Abstract
The encoding product of Chordin-like 2 (CHRDL2) is a member of the chordin family of proteins, which has been shown to be aberrantly expressed in several types of solid tumors. The regulatory underlying mechanisms of CHRDL2, however, remain poorly understood in gastric cancer (GC). In the present study, we determined that CHRDL2 was abnormally upregulated in human gastric cancer tissues compared with adjacent normal tissues. We also showed that CHRDL2 was positively associated with T stage, the pathological stage, distant metastasis, and poor patient prognosis. Moreover, we verified that overexpressing CHRDL2 promoted the proliferation and cell cycle transition of GC cells both in vitro and in vivo, whereas the opposite results were observed in CHRDL2-depleted cells. In addition, the expression levels of Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) were increased, and the levels of MST2 were decreased in CHRDL2 overexpressing cells. Consistent with previous findings, we observed the converse results in CHRDL2-silenced GC cells. Additionally, knockdown of YAP and overexpression of STK3 (MST2) could reverse the effects of CHRDL2 overexpression-induced proliferation of GC cells in vitro. Taken together, CHRDL2 plays a key role by activating the Hippo/YAP pathway in gastric cancer. Therefore, CHRDL2 could serve as a potential therapeutic tool for the treatment of gastric cancer.
An effect of digital rehabilitation on patients with total hip arthroplasty during the first-month hospital stay in China
Authors: Zhang Chi , Zhang Xin , Zhang Zhean , Ferland Amanda , Li Hua , Yang Yu , Ding Lu 
Published: April 2022

Abstract
Background and Objectives: The video-based physical therapy has shown the promising enhancements in rehabilitation from total hip arthroplasty in the past decade. However, the critical question was whether patients 50 years old and above in China could follow the instructions from mobile application well? This study attempted to answer this question. Methods: One hundred twenty patients with total hip arthroplasty participated in this study. They are randomly assigned into two groups: digital physical therapy (DPT) and conventional physical therapy (CPT) group. These two groups received similar exercise/balance interventions based on but not limited to the suggestions from the American Academy of Orthopedic Surgery in the first-month hospital stay. Five measures were used as follows: Lower Extremities Functional Score, Harris Hip Score, Ten-meter walking test, Timed-Up & Go, and Berg Balance Scale. Results: Compared to CPT group, DPT group demonstrated similar or even better performance in the abovementioned measure, specifically in Berg Balance Scale (p = 0.005). Conclusions: The results clearly showed that DPT was a feasible rehabilitation for future home-based rehabilitation for patients with total hip arthroplasty. Furthermore, our results also demonstrated that the DPT might be more effective than CPT in China due to the unlimited training opportunities after THA. In addition, it would be worthwhile to investigate the cost-effectiveness of this DPT.
Website Reorganization using Physics based Algorithms
Authors: Kumar Santosh , Goyal Aman Kumar , Sharma Arjun , Verma Arpit 
Published: April 2022

Abstract
This is the era of the internet and more than 50 percent of the population is dependent on it. Internet is collection of web pages which can be transported across it. Its size is very huge and increasing exponentially day by day. Now, around 30 percent of the overall content is duplicate. Finding relevant and required information from such a huge source of information is a very challenging task and finding top ranked results is a NP-hard problem. Majorly, the overall content can be categorized in three parts: content data, usage data and structure data. Web mining techniques and metaheuristic algorithms have been successfully applied in the literature to extract the useful information. This paper aims to provide ranking to the web links which could be utilized for restructuring of websites for business intelligence. Different features like keywords frequency, user’s navigation behaviour like unique visitors, duration stayed, access frequency, hub, and authority information has been utilized for prioritizing top-T web links. These results may be useful in different applications that include web personalization, website reorganization, recommendation systems, search engine optimization, etc. Other than this, it will also help in improving the user’s experience on websites. Based on different types of data, algorithms WLRGA, WLRBBBC, and WLRGbSA have been proposed for finding top-T web links and experimentally it has been seen that WLRGbSA is able to select top quality web links useful specially for website reorganization.
Donor hematopoietic stem cell/lymphocyte as maintenance treatment after CAR T-cell therapy in B-cell acute lymphoblastic leukemia relapsed after stem cell transplant
Authors: LI Qing , Cuicui LYU , DENG Haobin , LIU Meijing , Jia WANG , Jijun YUAN , Haibo ZHU , JIANG Yanyu , Erlie JIANG , ZHANG Rongli , DENG Qi 
Published: April 2022

Abstract
Background: Maintaining the efficacy of anti-CD19 chimeric antigen receptor modified (CAR) T-cell therapy in patients with B-cell acute lymphoblastic leukemia (B-ALL) relapsed after transplantation is an urgent problem.Methods: Overall 22 B-ALL patients who relapsed after allogenic hematopoietic stem cell transplantation (allo-HSCT) received anti-CD19-CAR T-cell therapy. Patients who responded to CAR-T cell therapy received donor hematopoietic stem cell infusion (DSI) or donor lymphocyte infusion (DLI) as maintenance therapy. We compared the clinical responses, acute graft versus host disease (aGVHD), expansion of CAR-T cells, and adverse events between two groups. Results: CR or CR with incomplete count recovery was achieved in 19 patients (86.4%). After DSI/DLI therapy, grade I-II of aGVHD was observed in 4 patients (36.4%) in DSI group. Only one patient developed grade II aGVHD in DLI group. The peaks of CAR T-cells in DSI group were higher than in DLI group. IL-6 and TNF-α levels increased again in 9 of 11 patients after DSI but not in DLI group. Progression-free survival and overall survival were higher in DSI group than in DLI group at 365 days. Conclusions: For B-ALL patients who relapsed after allo-HSCT, DSI is a feasible maintenance therapy if CR is obtained with CAR-T cell therapy.Trial registration: ChiCTR-ONN-16009862 Registered14November2016,https://www.chictr.org.cn/showproj.aspx?proj=16756ChiCTR1800019622 Registered24November2018,https://www.chictr.org.cn/showproj.aspx?proj=33185
Involvement of miR-31, miR-148a, &amp; miR -221 as three risky microRNAs in the invasion and angiogenesis of glioblastoma cells via myelinated nerve fiber of white matter tracts path
Authors: Shahkileh Seyedeh Sahar Valinejad , Ghaderian Sayyed Mohammad Hossein , Sheikhha Mohammad Hasan , Mohammadpour Hadiseh , Rakhshan Azadeh , Bastami Milad , Vojdani Samaneh 
Published: April 2022

Abstract
Glioblastoma is one of the most frequently occurring and malignant brain tumors. Due to the importance of microRNAs and HIF1α gene that is involved in the angiogenesis and growth of tumor cells, this study aimed to evaluate the correlation of the expression of HIF1α gene with miR-148a, miR-31, and miR-221, which were reported by in silico analysis as risky microRNAs in GBM. The findings obtained from Real-Time PCR using TaqMan Assay indicated the significant difference in the expression of HIF1α gene and microRNAs between the patient and healthy groups and the expression changes were obtained point to point in the patients; so they can be considered as important biomarkers in glioblastoma. The obtained AUC from the ROC analysis indicated that the analysis of the expression of HIF1α and miR-148a, miR-31 & miR-221 genes can be used to distinguish healthy and patient groups nevertheless, the correlation of HIF1α gene expression with the microRNAs was reported low according to Pearson’s correlation coefficient. Thus the significant increase in HIF1α gene expression is probably controlled by other molecules. By using bioinformatics analysis; Gene Ontology biological process; MSigDB Hallmark and KEGG pathway enrichment analysis of PPI network was carried out with Enrichr web-based application. The axon guidance pathway, proteoglycan in cancer and some of intracellular signaling pathways identified as the most important signaling pathways. The results of MSigDB_Hallmark pathway enrichment analysis introduced hypoxia signaling pathway with less involvement from these micoRNAs. Therefore these microRNAs can be considered in Glioblastoma cell infiltration through the myelinated nerve fibers of white matter tracts.
A Retrospective study on constrictive pericarditis and suggestions on diagnostic criteria
Authors: Li Junfang , Li Rong , Cheng Guangting , Lu Changhong , Liu Weigang , Sun Dongmei , Li Xue , Wang Zhibin 
Published: April 2022

Abstract
Background: The diagnosis of constrictive pericarditis (CP) is a challenge. There is no standard echocardiographic diagnosis criteria of CP yet. This study analyzed and summarized the features of 25 patients with CP, and then proposed suggestions of diagnostic criteria. It is hoped that the suggestions may help professionals make decisions in their daily practice, and patients get timely diagnosis and effective treatment.Methods: Twenty-five patients with CP were selected for the retrospective study. The clinical and echocardiographic imaging data were analyzed and summarized. At end of the article, suggestions on diagnostic criteria of CP were put forward.Results: The main clinical manifestations were fatigue, breathlessness and exertional dyspnea (88%) and signs of edema of lower limbs (84%), hepatomegaly and jugular vein filling (84%). Echocardigraphic features included pericardial thickening (88%) and calcification (60%), pulmonary hypertension (52%), IVC dilation (80%) , left and/or right atrial enlargment (100%), diastolic flattening of the LV posterior wall (72%), septal shudder and bounce (64%), restrictive LV and RV diastolic filling pattern (100%), early filling changes of mitral and tricuspid flow (80% and 60% respectively), mitral annulus reversus (73%).Conclusions: Echocardiography is a simple and valuable examination for CP. The echocardiographic diagnostic criteria are valid and might be further promoted.
Impact of the Covid-19 pandemic on emergency department visits and hospital admissions in a tertiary hospital in Barcelona, Spain.
Authors: Aldecoa Victoria , Madrid Paula , Cahis Nuria , Ranera Adrian , Moya Sandra , Baena Irene , Corripio Raquel 
Published: April 2022

Abstract
BACKGROUND: describe the changes observed in admissions and visits to the pediatric emergency department (ED) due to the lockdown during the COVID-19 pandemic. METHODS: cross-sectional observational study on pediatric ED and hospital admissions comparing two periods (March 9th-May 9th, 2019 and March 9th-May 9th, 2020). RESULTS: 6568 ED visits (53.9% male; mean age 6.5±5.1) were recorded in 2019 and 1133 (55.2% male; mean age 7.3±5.6) in 2020, showing a decrease of 82.7% (81.8 – 83.7%). Severity of patients, procedures and length of stay in ED were significantly higher during the COVID-19 period. Although hospital admissions decreased during the lockdown (-55.1%, p<0.001); 7.9% of ED patients were admitted to the hospital over the study period in 2020 vs 3.0 % in 2019; OR=2.61 (2.03 – 3.35), p<0.001. Hospital admissions related to infectious diseases decreased; OR=0.57 (0.33 – 0.95) and trauma and surgery increased; OR=1.99 (1.16 – 3.41), length of stay remained stable. CONCLUSION: the effects of lockdown during the COVID19 pandemic were translated into a significant decrease of ED visits (83%) and hospital admissions (56%), although hospital admission rate increased (OR=2.61). Hygienic measures, managing symptoms at home and fear of coming to the hospital could explain these changes. Telemedicine and public health education interventions are mandatory in times of lockdown, as well as coordination between primary health care centers and hospitals.
Improving Hadoop Performance through scheduling and prefetching
Authors: EDDOUJAJI MOHAMED , SAMADI HASSAN , BOUHORMA MOHAMMED 
Published: April 2022

Abstract
In this paper, we continue investigating how to improve Hadoop performance , we will investigate further strategies, tackling the data locality with respect to data access patterns, cluster memory and effective scheduling. In a Hadoop cluster, users are generally accessing data according to their business needs, which make some data more accessed than others, reason why we consider data patterns as a crucial element in our approach. In fact, the performance can be affected by the latency of data access, which is best when blocks are already in memory while requested for processing, knowing that caching too much can lead to memory overhead or even severe delays when wrong data are always taking memory space. Actually, clusters are nowadays offering more capabilities in term of nodes memory, which is considered in most cases as highly underutilized according to several studies. In our approach we considered the cluster memory to improve efficiency by using the underutilized memory space in a way to offer better data locality for future tasks, through a new prefetching/scheduling algorithm. The idea behind our approach is to differentiate blocks by popularity based on the previous access patterns, then proceed by memory allocation or memory eviction in optimal and efficient way across all the cluster.
Design and Fabrication of Sierpinski Square Fractal Microstrip Patch Antenna with Parasitic Elements for Wideband Applications
Authors: Kattimani Bharamappa , Patil Rajendra R 
Published: April 2022

Abstract

The narrow bandwidth of a microstrip antenna is one of its key drawbacks. This work shows how a dual-layer stacked and parasitic arrangement, together with the effect of fractal designs, can provide wide bandwidth and high gain qualities in a microstrip patch antenna. For wideband applications, the proposed antenna design in stacked construction has a total bandwidth of 750MHz for S11 -10dB. In order to reduce the antenna size, the Sierpinski square carpet fractal is used. This antenna exhibits a lower resonance frequency due to the space-filling property of fractal geometry. The resonance frequency of this patch antenna drops as iteration and iteration factors rise, according to experimental results. Wideband functioning is achieved with a size decrease of 70.1%. The proposed antenna design exhibits a 6.98dB gain. These qualities of wideband and higher gain make the proposed antenna an excellent one for wideband applications.