bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myeloid CCR2 Promotes Atherosclerosis after AKI
Authors: Hüsing Anne M. , Wulfmeyer Vera C. , Gaedcke Svenja , Fleig Susanne V. , Rong Song , DeLuca David , Haller Hermann , Schmitt Roland , von Vietinghoff Sibylle 
Published: May 2022

Significance Statement
AKI impairs excretory function, but also leaves a damaged kidney. We demonstrate that a single episode of unilateral ischemia-reperfusion injury significantly promotes atherosclerotic plaque formation in mice. Renal inflammation preceded expression of myeloid and T cell genes in the atherosclerotic aorta. The chemokine receptor CCR2 was instrumental in inflammatory monocyte recruitment to the kidney, renal and aortic inflammatory macrophage marker CD11c expression, and enhanced aortic plaque formation after AKI. Delineating underlying mechanisms in atherosclerosis support the concept of a “toxic kidney” that promotes remote inflammation after ischemic reperfusion injury.

Background
The risk of cardiovascular events rises after AKI. Leukocytes promote atherosclerotic plaque growth and instability. We established a model of enhanced remote atherosclerosis after renal ischemia-reperfusion (IR) injury and investigated the underlying inflammatory mechanisms.

Methods
Atherosclerotic lesions and inflammation were investigated in native and bone marrow–transplanted LDL receptor–deficient (LDLr−/−
) mice after unilateral renal IR injury using histology, flow cytometry, and gene expression analysis.

Results
Aortic root atherosclerotic lesions were significantly larger after renal IR injury than in controls. A gene expression screen revealed enrichment for chemokines and their cognate receptors in aortas of IR-injured mice in early atherosclerosis, and of T cell–associated genes in advanced disease. Confocal microscopy revealed increased aortic macrophage proximity to T cells. Differential aortic inflammatory gene regulation in IR-injured mice largely paralleled the pattern in the injured kidney. Single-cell analysis identified renal cell types that produced soluble mediators upregulated in the atherosclerotic aorta. The analysis revealed a marked early increase in Ccl2, which CCR2+ myeloid cells mainly expressed. CCR2 mediated myeloid cell homing to the post-ischemic kidney in a cell-individual manner. Reconstitution with Ccr2−/−
bone marrow dampened renal post-ischemic inflammation, reduced aortic Ccl2 and inflammatory macrophage marker CD11c, and abrogated excess aortic atherosclerotic plaque formation after renal IR.

Conclusions
Our data introduce an experimental model of remote proatherogenic effects of renal IR and delineate myeloid CCR2 signaling as a mechanistic requirement. Monocytes should be considered as mobile mediators when addressing systemic vascular sequelae of kidney injury.
El papel de la competencia pragmática en la enseñanza del idioma inglés
Authors: Pirogova Nadezhda 
Published: May 2022

El presente artículo analiza el papel de la pragmática en el ELT (English Language Teaching) en el estudio de cómo los estudiantes entienden y usan el lenguaje en contextos específicos. Para convertirse en usuarios de idiomas eficientes y competentes, los alumnos tienen que estudiar más que formas y significados lingüísticos. La exposición al lenguaje es insuficiente para la adquisición de habilidades pragmáticas, por lo que las observaciones de los estudiantes de idiomas muestran que existe una gran necesidad de enseñar pragmática. La investigación se centra en el desarrollo de competencias pragmáticas que incorporan conocimientos lingüísticos y culturales, y se presentan diferentes razones para ayudar a los alumnos a mejorar su competencia pragmática. Este estudio tiene un diseño experimental y su objetivo principal es mejorar la competencia pragmática de los estudiantes en inglés. Se llevó a cabo en un grupo mixto de veinte estudiantes que estudian inglés en una universidad. Los resultados de la investigación indican que el enfoque explícito en la pragmática en el aula conduce a mejoras en el desarrollo pragmático.
Un tipo de publicación científica poco explorada por investigadores peruanos
Authors: Fernández-Bedoya Víctor Hugo , Suyo Vega Josefina Amanda , Meneses la Riva Monica Elisa , Grijalva Salazar Rosario Violeta 
Published: May 2022

El perfil del docente universitario es caracterizado por ser una persona académica, quien generalmente combina actividades ligadas a la investigación, a la enseñanza y a veces realiza tareas profesionales en la universidad como gestión o trabajo clínico (van Dijk et al., 2020). Dentro de las actividades ligadas a la investigación, la producción de publicaciones científicas a través de revistas indexadas en bases de datos de calidad es un indicador mediante el cual se mide la gestión de la investigación universitaria. Gracias a ello, es conocido que las universidades peruanas han logrado triplicar su producción de publicaciones científicas según el Ranking Scimago Iberoamericano 2021, con relación al del 2015.
Uno de los tipos de publicación científica menos explorados en Perú son las cartas al editor (en adelante CE). Estas son consideradas manuscritos cortos, utilizados para la presentación de comentarios relevantes en una temática, o para realizar un comentario o un análisis crítico de un artículo previamente publicado en una revista, inclusive pueden ser utilizados para comunicar hallazgos pequeños que debido a sus características no calzan en otros formatos de publicación en una revista (Pascual-Huerta, 2017).
Mechanical Properties of Multi-walled Beryllium-Oxide Nanotubes: A molecular Dynamics Simulation Study
Authors: Rostamiyan Yaser , Shahab Navid , Spitas Christos , Mashhadzadeh Amin Hamed 
Published: May 2022

Abstract
Molecular Dynamic (MD) simulation was employed to take the molecular fingerprint of mechanical properties of Beryllium-Oxide nanotubes (BeONTs). In this regard, the effect of the radius, the number of walls (single-, double- and triple-walled), and interlayer distance, as well as temperature on the Young’s modulus, failure stress and failure strain are visualized and discussed. It was unveiled that larger single-walled BeONTs have lower Young’s modulus in zigzag and armchair direction, and the highest Young’s modulus were obtained for the (8,0) zigzag and (4,4) armchair SWBeONTs as of 645.71GPa and 624.81GPa, respectively. Unlike Young’s modulus, however, the failure properties of the armchair structures were higher than those of zigzag ones. Furthermore, similar to SWBEONTs, an increase in the interlayer distance of double-walled BeONTs (DWBeONTs) led to a slight reduction in Young’s modulus value, while no meaningful trend was found among failure behavior. For double-walled BeONTs (TWBeONTs), the elastic modulus was obviously higher in both armchair and zigzag directions compared to DWBeONTs.
PV-DC Double Controller Scheme
Authors: Uzorka Afam 
Published: May 2022

Abstract
Dynamic model simulation and implementation of a photovoltaic solar powered Permanent magnet DC motor (PMDC) controlled by dual chopper action are studied. The simulation results of the PMDC motor and resistive load excursion are discussed. In order to maximize the power output from the solar array and to make the energy system fully stable under varying photovoltaic condition and load excursions two different controllers developed by Dr. A.M. Sharaf are validated. The basic description of the photovoltaic system and dual controllers are given. The dynamic validation results with and without the controllers are given. The validation results shows an increase in the photovoltaic output power.
Ligand GA: a genetic algorithm for automated protein inhibitor design
Authors: Chalmers Gordon 
Published: May 2022

Abstract
Ligand GA is introduced in this work and approaches the problem of finding small molecules inhibiting protein functions by using the protein site to find close to optimal or optimal small molecule binders. Genetic algorithms (GA) are an effective means for approximating or solving computationally hard mathematics problems with large search spaces such as this one. The algorithm is designed to include constraints on the generated molecules from ADME restriction, localization in a binding site, specified hydrogen bond requirements, toxicity prevention from multiple proteins, sub-structure restrictions, and database inclusion. This algorithm and work is in the context of computational modeling, ligand design and docking to protein sites.
The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic.
Authors: Carvajal-Oliveros Angel , Uriostegui-Arcos Maritere , Zurita Mario , Melchy-Perez Erika I. , Narváez-Padilla Verónica , Reynaud Enrique 
Published: May 2022

Abstract
SH-SY5Y is a cell line derived from human neuroblastoma. It is one of the most widely used in vitro models to study Parkinson’s disease. Surprisingly, it has been found that it does not develop a dopaminergic phenotype after differentiation, questioning its usefulness as a Parkinson’s model. There are other in vitro models with better dopaminergic characteristics. BE (2)-M17 is a human neuroblastoma cell line that differentiates when treated with retinoic acid. We compared the dopaminergic and serotonergic properties of both cell lines. BE (2)-M17 has higher basal levels of dopaminergic markers and acquires a serotonergic phenotype during differentiation while maintaining the dopaminergic phenotype. SH-SY5Y has higher basal levels of serotonergic markers but does not acquire a dopaminergic phenotype upon differentiation.
Paenibacillus arenilitoris sp. nov., isolated from seashore sand and genome mining revealed the biosynthesis potential as antibiotic producer
Authors: Deng Na , Huang Huiqin , Hu Yonghua , Wang Xu , mo kunlian 
Published: May 2022

Abstract
Strain IB182493T, a marine, aerobic, Gram-stain-negative and motile bacterium, was isolated from seashore sand of South China Sea. Cells grew optimally at 25–30°C, pH 7.0–8.0 and in 2–4 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Paenibacillus, and was most closely related to Paenibacillus harenae DSM 16969T (similarity 96.6%) and Paenibacillus alkaliterrae DSM 17040T (similarity 96.1%). The chemotaxonomic characteristics of strain IB182493T included MK-7 as the predominant isoprenoid quinone, anteiso-C15:0 and iso-C16:0 as the major cellular fatty acids and meso-diaminopimelic acid as the diagnostic diaminoacid in cell wall peptidoglycan. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. The DNA G+C content of strain IB182493T was 56.2 mol%. The values of whole genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between the isolate and the closely related type strains were less than 84.7 % and 23.6 %, respectively. On the basis of phenotypic and chemotaxonomic properties, phylogenetic distinctiveness and genomic data, we named strain IB182493T as Paenibacillus arenilitoris sp. nov. and proposed that strain IB182493T (=MCCC 1K04626T = JCM 34215T) in the genus Paenibacillus represents a novel species.
Cholinergic signals promote esophageal squamous cell carcinoma metastasis by activating EGFR/AKT signaling pathway
Authors: Wang Tao , Ge Bei-Bei , Liu Chang , Xu Li-Juan , Wei Bin , Gao Yong 
Published: May 2022

Abstract
Purpose Increasing evidence has shown that the nervous system plays a vital role in the regulation of tumorigenesis. However, the regulatory mechanism of the parasympathetic nervous system in the progression of esophageal squamous cell carcinoma (ESCC) has not been elucidated. Methods Vesicular acetylcholine transporter (VAChT) protein expression was detected by immunohistochemistry (IHC) and parasympathetic nerve density was analyzed in human ESCC tissues. The co-culture model of ESCC cells and dorsal root ganglia (DRG) was established in vitro. The effects of cholinergic stimulation on cell growth, migration and invasion were investigated through CCK-8, Transwell and wound healing assays using acetylcholine (ACh). PCR and western blotting were utilized respectively for the purpose of detecting Epithelial-to-mesenchymal transition (EMT) related transcripts proteins. M3 muscarinic receptor (CHRM3) was knocked down using small interfering RNA (siRNA). RNA sequencing explored the possible altered gene transcriptome and signal pathways, which were further verified by Western blotting and CO-IP analysis. Results The intensity of parasympathetic nerve in ESCC was significantly higher than that in adjacent tissues, and was positively correlated with higher clinical staging and poorer prognosis. Co-incubation with DRG enhanced the migration and invasion ability of ESCC cells in vitro. Moreover, exogenous choline stimulation enhanced the migration, invasion ability together with EMT of ESCC cells, while CHRM3 knockdown were opposite. Mechanistically, cholinergic activation enhanced the phosphorylation levels of EGFR and AKT through CHRM3, which bound directly to EGFR.Conclusion We revealed a critical role of cholinergic signals ACh/CHRM3 in mediating the effects of parasympathetic nerve activity on ESCC through the EGFR/AKT signaling pathway, which might provide a novel therapeutic target for ESCC.
Quality of Family Planning Among Married Women at Ngo Supported and Non-supported Districts in Bati Woreda Oromia Special Zone Amhara Region, Ethiopia, 2021
Authors: Abebaw Nigusie , Girma Natnael , Berhanu Wosensew , Tefera Zenebe 
Published: May 2022

Abstract
Background: Good quality of family planning service helps individuals and couples meet their reproductive health needs safely and effectively. This study may provide important information to family planning providers, policy makers, and program managers to improve quality of family planning service.Objective: To assess quality of family planning among married women at NGO supported and non-supported in Bati Woreda Oromia special zone Amhara region, Ethiopia, 2021.Method: Institutional based comparative cross-sectional study was conducted during study period. Data was collected from March 1-30/2021 by using 344 sample size . Data were entered to Epi Data 4.1 and exported to SPSS version 20.0 for analysis. Adjusted odds ratio with 95%Confidence interval was used to measure association variable with p-value <0.05 Result: 344 participants were enrolled to this study making the response rate of 100 %.This finding showed that , 63.3% were supported and 58.1% non-supported by NGO. The mean age was 36 SD + 6.17 years old with range between 23-49 years. Contraceptive users who are supported by NGO [95% CI, 2.17(1.19-6.13)],Short waiting time [95% CI, 1.276(0.54-2.99)], Availability of different choice, [95% CI, 0.3(0.12-0.71)], Good counseling, [95% CI, 0.04(0.0-0.0043)] and Keeping privacy for contraceptive users[95% CI,0.21(0.08-0.48)] were significantly associated with the dependent variable. Conclusion: Supported status, waiting time availability of method, provider good service and Clear and satisfactory counseling were the most determinant factor for the dependent variable. Therefore, all health care provider gives clear and precise about contraceptive method.
Influence of microalgal diets in the mass production and nutrient enrichment of Apocyclops royi (Cyclopoida, Copepoda) - A potential live food for marine fish larvae
Authors: Vijayaraj Radha , Jayaprakshvel Mani , Altaff Kareem 
Published: May 2022

Abstract
This study investigated the effects of three microalgal diets (Chloroidium saccharophilum, Chlorella vulgaris and Nannochloropsis oculata) on the population growth, nutritional composition of Apocyclops royi. The microalgal strains were taxonomically identified using 18S rRNA Technique. This copepod is mass cultured with C. saccharophilum diet is identified as potential live prey for marine finfish larval rearing. Under optimal water quality parameters and microalgal diet of C. saccharophilum, C. vulgaris and N. oculata at the concentration of 30,000 cells/mL, the A. royi showed high survival and ovisac production with the C. saccharophilum feeding regime. Mass culture of this copepod reached a density of (12700 ± 454 ind./L). on 21st day of culture period. The fatty acid profile showed enhanced content of saturated and PUFAs in A. royi (p < .05) with C. saccharophilum diet. The contents of EPA, DHA and ARA in A. royi are high with C. saccharophilum diet compared to other microalgal diets. Amino acid profile of A. royi fed C. saccharophilum showed significantly high content of Glycine, Alanine, Aspartic acid, Glutamic acid, Threonine and Histidine. The total essential amino acid content is significantly higher than the animals fed on C. vulgaris and N. oculata (p < .05). Our findings illustrate that the C. saccharophilum is the most suitable microalgal diet for mass culture of A. royi because it increases productivity and enhances the nutritional value of the copepods for use as fish larvae prey.
Design and Construction of Heterojunction-type TiO2-CdS Photocatalyst on Bacterial Cellulose with Enhanced Photocatalytic Activity
Authors: Qian Xin , Xu Yongjian , Xu Yang 
Published: May 2022

Abstract
Photocatalytic degradation, a green and promising technology, has been extensively explored to reduce environmental pollutions. Herein, a Z-scheme TiO2-CdS photocatalyst with binary hierarchical structural was designed and constructed on bacterial cellulose (BC) via microwave-assisted solvothermal in-situ synthesis.The as-prepared BC@TiO2-CdS nanocomposite gel possesses enhanced photocatalytic activity with 94.47% removal of methylene blue (MB) after 180 min visible light irradiation. The enhancement of photocatalytic activity is attributed to the hierarchical structure of the TiO2-CdS nanospheres on BC, which was characterized and confirmed by SEM, EDS, XRD, XPS and Raman spectral. Meanwhile, the photoluminescence (PL) analysis reveals that the BC@TiO2-CdS nanocomposite gel follows the direct redox Z-scheme photocatalytic reaction system, which is advantageous for photogenerated charge separation and migration from TiO2 conduction band (CB) to CdS valence band (VB). Therefore, this work provides a facile route to design and construct a direct Z-scheme system for photocatalytic degradation.
Performance and increase fatigue life for Ti workpiece produced by EDM process
Authors: Liu Zhi-wei , Hsu Chung-Wei , Wu Shang-Hua , Zhou Jia-Sheng , Kao Jin-Yih , Hsu Chun-Yao 
Published: May 2022

Abstract
Effect of discharge current (range from 5 to 13 A) in electrical discharge machining (EDM) on the machining characteristics of pure Ti is investigated. The electrode wear rates (EWR), material removal rates (MRR), surface roughness (SR), micro-hardness, fatigue life and recast layer are measured. Increasing discharge current, the EWR, MRR, SR and the recast layer thickness also increases. Fatigue life is positively correlated with the micro-hardness of EDMed workpiece. Control charts are used to ensure the EDM process is well regulated and stable. EDMed surface is modified by plasma etching, the surface defects are gradually removed. EDMed surface coated with a TiAlN film has better surface quality and a significantly greater fatigue life.
Stakeholder’s perspective on the slow progress towards elimination of trachoma as a public health problem, and suggested recommendations for future intervention improvements: A pilot case study in Bugna district, 2020, Northeast Ethiopia
Authors: Kassaw Mesfin Wudu , Tegegne Kindie Mekuria , Ahmed Mohammed , Tegegne Kirubel Dagnaw 
Published: May 2022

Abstract

Background: Blinding Trachoma is considered a neglected tropical disease and earmarked for global elimination as a public health problem by 2020 using the advocated World Health Organization's (WHO) recommendation, SAFE (surgery, antibiotics, facial cleanness, and environmental change) strategy. In Sub-Saharan Africa, Trachoma is a leading cause of preventable blindness, and Ethiopia is the most affected and bears the highest burden. In many rural parts of Ethiopia, Trachoma continues to be rampant. In the North and South Wollo zones of the Amhara region, the overall prevalence of active Trachoma among children aged 1–9 was 21.6%. In the Gazegibela district of Wagehemra zone, Amhara region, among children aged 1–9 years, 52.4% were positive for active Trachoma. This study aimed to explore the determinates of SAFE strategy failure when WHO and MoH implement a SAFE strategy to control Trachoma from 2006 until 2020 and reconsider the recommendation of stakeholders in Bugna district, Ethiopia. Methods: The design used in this study is an exploratory case study to highlight the determinants of SAFE strategy failure. The data were collected in Bugna district, Northeast Ethiopia. The study data were collected from Health Extension Workers (HEWs) and kebele leaders. We employed an in-depth interview and Focus Group Discussion (FGD) to gain access to the participants' experiences and conducted an inductive qualitative content analysis. The study population includes 28 health extension workers, 16 kebele leaders, 2 district health office leaders, 2 district trachoma officers, and 5 district health officers. Both the interview and FGD sessions were audio-taped, and an interview lasted one to two hours, but the FGD lasted two to three hours. Result: The study subjects live in 16 kebeles (sub-districts) of the Bugna district. The age of health extension workers ranged from 23 to 35 years old, with a mean age of 30.57 years (SD±2.68). Participants' opinions were categorized into two major categories; determinants for failing in controlling Trachoma in the Bugna district and forwarded recommendations to reduce the prevalence of trachomatous inflammation-follicular(TF) below 5%, and the prevalence of trachomatous trichiasis (TT) below 0.2%. In these two major categories, four themes were identified as determinants for failing to control Trachoma below 5% in the Bugna district in 2020, while six themes were deduced as recommendations to reduce the prevalence of TF below 5% and TT below 0.2% in Bugna district post-2020.Conclusion: Many organizations like The Carter center played a great deal and still investing their time, money, and knowledge to control TF below 5% and TT below 0.2% as per WHO recommendation through global elimination of Trachoma by 2020 (GET2020).