bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated admission blood glucose is an independent risk factor for 30-Day major adverse cardiovascular events in STEMI patients, but not in NSTEMI patients
Authors: Li Yanan , Wang Qinshi , Pei Junyu , Wang Xiaopu , Zheng Keyang , Zhang Ping , Yan Jiafu 
Published: May 2022

Abstract
BackgroundElevated admission blood glucose was associated with adverse outcomes in patients with acute myocardial infarction (AMI). However, no further studies investigated the association between admission blood glucose and 30-day major adverse cardiovascular outcomes (MACEs) in ST-segment elevation myocardial infarction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) patients, respectively.ObjectivesThe purpose of this study was to evaluate the impact of admission blood glucose on the risk of 30-day major adverse cardiovascular events in AMI patients, and to assess the difference in outcome between STEMI and NSTEM patients.Methods and ResultsThis study was a post hoc analysis of the Acute Coronary Syndrome Quality Improvement in Kerala (ACS-QUIK) Study, and 13,398 participants were included in the final analysis. Participants were divided according to the admission blood glucose quintiles. Compared with participants with admission blood glucose between 5.4 and 6.3 mmol/L, participants with the highest quintile of admission blood glucose (≥ 10.7 mmol/L) were associated with increased risk of 30-day MACEs in the fully adjusted logistic regression model [adjusted OR (95%CI): 1.86 (1.36, 2.56), P<0.001]. There was a positive linear association between admission blood glucose and the risk of 30-day MACEs in AMI patients [adjusted OR (95%CI): 1.05 (1.03, 1.07), P<0.001]. This trend was more significant in patients with STEMI (P for interaction = 0.016) or patients below 65 years old (P for interaction = 0.025).ConclusionIn patients with AMI, elevated admission blood glucose was associated with the increased risk of 30-day MACEs, and this relationship was found only in STEMI patients, not in NSTEMI patients.
Analysis of Phylogeny of Agrocybe Genus Based on Nucleotide Database in NCBI
Authors: Zhou Ping Qing , Li Li Ling , Shi Hua Guo , WANG Long , Li Yang 
Published: May 2022

Abstract
The study of the phylogeny of species is a basic discipline for the effective protection, development, and utilization of their germplasm resources. Since this century, the fungi of the Agrocybe genus have become the most successful class of medium and high-grade edible and medicinal fungi that have been artificially domesticated and grown on a large scale, and have high commercial value. However, there are few reports on its germplasm resources. To clarify the genetic and developmental relationship at the genus level of the Agrocybe in the world. In this paper, Based on the NCBI Nucleotide database(National Center for Biotechnology Information), taking the single gene ITS sequence as the research object, the T92 (Tamura 3-parameters) parameter model was used to calculate the genetic distance, and the Neighbor-Joining Method was used to construct the genus-level phylogenetic tree of the Agrocybe. The results showed that the phylogenetic tree constructed by 192 ITS single gene sequences of 43 species of the Agrocybe (including 31 known species and 12 undetermined species) in the NCBI Nucleotide database was roughly divided into 6 branches and belonged to different groups, and 17 undetermined species of the Agrocybe sp. was preliminarily identified. This study has a certain reference value for the genetic relationship among different species of Agrocybe all over the world, as well as the selection of the parent bank and the protection of germplasm resources of each species of the genus.
circ_0052184 promote colorectal cancer progression via targeing miR-604/HOXA9 axis
Authors: Huang Yandong , Bai Qinyang , Wang Zhanlong , Yu Hongbo , Li Yanru , Lu Hao , Kang Huimin , Shi Xuewei , Feng Kai 
Published: May 2022

Abstract
Background:Mortality rate colorectal cancer (CRC) ranks second. In a variety of diseases CircRNAs are abnormal expression, and their dysregulation is associated with cancer progression. Recent studies have shown that the malignant progression of colorectal cancer cannot be separated from the abnormal expression of circRNAsMethods:First, the circ_0052184 expression in clinical tissue and cell samples was analyzed by database analysis and qRT-PCR. qRT-PCR also assessed Construction of circ_0052184-silenced colorectal cancer cells. Furthermore, the proliferation ability of cells was detected by MTT method. Cell migration ability was tested by wound healing assay. Cell migration and invasion ability were detected by transwell method.Results:In cell lines and tissues of colorectal cancer circ_0052184 is significantly overexpressed. Proliferation, migration and invasion of colorectal cancer cell are inhibited by circ_0052184 silencing. The existence of the circ_0052184/miR-604/HOXA9 regulatory network was verified. Moreover, the existence of this regulatory network has an impact on the occurrence and development of colorectal cancer.Conclusions:Our study demonstrated the molecular mechanism by which circ_0052184 regulates the miR-604/HOXA9 axis for the first time. This mechanism promotes the malignant progression of colorectal cancer cells.
Evidence of aerosol transmission of African swine fever virus in piggeries under field conditions: a case report
Authors: Li Xiaowen , Hu Zhiqiang , Fan Mingyu , Wu Weisheng , Gao Wenchao , Bian Lujie , Liu Wenhao , Tian Xiaogang , Jiang Xiaoxue , YAN Zhichun Jason 
Published: May 2022

Abstract
BackgroundAfrican swine fever (ASF) is a devastating and economically significant infectious disease and has caused huge losses in the commercial pig sector in China since 2018. The major transmission route of African swine fever virus (ASFV), the causative agent of ASF, is direct pig-to-pig contact or indirect contact with virus-contaminated objects. Although aerosol transmission route of ASFV has been reported under experimental conditions previously, whereas no reports described under field conditions.Case presentationIn the three ASFV-positive piggeries, environmental samples, including floors, aerosols, dusts and air outlets, tested positive in the monitoring period. Aerosol and floor samples always tested positive firstly at the same time. ASFV-positive aerosols were associated with live ASFV-positive pigs in piggeries. Dust and air outlet samples remained positive for several days after all positive pigs were eliminated, and the times of positive and persistent positive of dust and air outlet samples were always close.ConclusionsThis is the first report to provide some evidence of airborne transmission route of ASFV in field piggeries, and more research is needed to study aerosol-spread laws of ASFV under field conditions, assess the risk of ASFV aerosol transmission, and develop effective strategies for air disinfection to create low-risk fresh air for pig herds.
Single-cell transcriptomic analysis deciphers key transitional signatures associated with oncogenic evolution in human intramucosal esophageal squamous cell carcinoma
Authors: Liu Xin-Yang , Liu Yan-Bo , Xu Jia-Cheng , Zhang Yi-Fei , Ruan Yuan-Yuan , Zhao Yi , Wu Lin-Feng , Hu Jian-Wei , Zhang Zhen , He Meng-Jiang , Chen Tian-Yin , Xu Xiao-Yue , Zhang Jing-Wei , Zhang Yi-Qun , Zhou Ping-Hong 
Published: May 2022

Abstract
Background: The early diagnosis and intervention of esophageal squamous cell carcinoma (ESCC) is particularly important due to poor prognosis and the lack of effective therapies. This study aims to systematically describe the cellular atlas of human intramucosal ESCC.Methods: Five paired samples of intramucosal ESCC, para-ESCC esophageal tissue from endoscopically resected specimen, and peripheral blood mononuclear cells were adopted for scRNA-seq analysis. A computational pipeline scMetabolism was applied to quantify the metabolic diversity of single cells.Results: A total of 164,715 cells were profiled. The abundance of CD8+ TEXs , Tregs and PD1+CD4+T cells suggested an exhausted and suppressive immune microenvironment. Several genes in immune cells such as CXCL13 and CXCR5 in PD1+CD4+ T cells and PADI4 in Mono2 cells were identified as new biomarkers for poor prognosis. Epithelial cells exhibited high intra-tumor heterogeneity and two evolutional trajectories during ESCC tumorigenesis initiated from proliferative cells, and then through an intermediate state, to two different terminal states of normal differentiated epithelial cells or malignant cells, respectively. Intercellular interaction analysis based on ligand-receptor pairs revealed malignant cells interacting with CAFs via the MDK-NCL pathway, which is an important hallmark in the change of tumor microenvironment, and serves as a sign of CAF activation to stimulate downstream pathways for facilitating tumor invasion, and therefore suggesting a potential early biomarker of ESCC progression.Conclusions: This study demonstrates the changes of cell subsets and transcriptional levels in human intramucosal ESCC, providing unique insights into the development of novel biomarkers and potential intervention strategies.
CYP4F2 1347C&gt;T and GGCX 12970C&gt;G Polymorphisms as Determinants of Stable Warfarin Dose in Sudanese Patients of Heart Valve Replacement
Authors: Elsadig Ahmed Elhadi , Elamin Elwaleed Mohamed , Muddathir Abdel Rahim Mahmoud , Ali May Mohammed , Sherif NassrEldin M. A. 
Published: May 2022

Abstract
Purpose
This study intended to explore the contribution of CYP4F2 1347C > T and GGCX 12970C > G polymorphisms on warfarin dose requirements among Sudanese subjects.
Methods
A total of 136 Sudanese patients of heart valve replacement receiving stable warfarin dose were recruited for this study. Blood samples were collected; DNA was extracted using phenol chloroform method. Genotyping for CYP4F2 1347C > T and GGCX 12970C > G polymorphisms was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. The association of genotype with warfarin dose requirement was measured by Kruskal Wallis and Mann Whitney U tests. Genotype, age, gender, comorbidity and concurrent medication were tested as predictors of stable warfarin dose using univariate regression analysis. For all tests, p. values < 0.05 were considered statistically significant.
Results
Frequencies of CYP4F2 1347C > T genotypes were 22.8% CC, 61% CT and 16.2% TT. The frequencies of C, T alleles were 0.533, 0.466 respectively which are deviated from Hardy Weinberg equilibrium (p. value = 0.008). Regarding GGCX 12970C > G genotyping, 96.9% were CC and 3% were CG. The frequencies of C, G alleles were 0.984 and 0.015 respectively which are in accordance with Hardy Weinberg equilibrium (p. value = 0.859). Insignificant differences in the mean daily warfarin dose between different genotype groups were observed (all p. values > 0.05). None of the studied variables was significant predictor of stable warfarin dose in this study population (p. values > 0.05).
Conclusion
No significant contribution of CYP4F2 1347C > T and GGCX 12970C > G polymorphisms on warfarin dose requirements was observed in this study population.
Effects of shape factors on biconvex compacts tensile strength measurement and their impact thereof on prediction of dilution potential from polynomial regression models of tensile strength-compaction force data
Authors: Ilyasu Salim , Olowosulu Adeniji Kehinde , Abdulsamad Abdulrahman 
Published: May 2022

Abstract
Purpose
To compare the effects of shape factors on biconvex compacts tensile strength measurement and their impact thereof on prediction of dilution potential from polynomial regression models of tensile strength-compaction force data of coprocessed diluents with similar deformation physics.
Methods
Compact of ibuprofen-diluent blends were produced between 4.98 to 29.89 kN in eccentric hydraulic press. Given the biconvex round compact dimensions (t = axial thickness, w = central cylinder thickness, and d = diameter), tensile strength was computed as \({\sigma }_{biconvex}=\mathcal{ }\mathcal{Q}\frac{F}{\pi {d}^{2}}\) where F, is the breaking force. The shape factors (\(\mathcal{Q}\)) were defined as \({\left[\frac{t}{2d}\right]}^{-1}\), \({\left[\frac{0.14t}{d}+\frac{0.36w}{d}\right]}^{-1}\), \(\left[10/\left[\left(\frac{2.84t}{d}\right) - \left(\frac{0.126t}{w}\right)+\left(\frac{3.15w}{d}\right)+0.01\right]\right]\) for Podczeck, Shang, and Pitt based models, respectively. Area under the curve (AUC) was obtained by second order polynomial fitting of the tensile strength-compaction force data followed by integration of the quadratic regression equation between the limits 4.98 to 29.89 kN. The AUC of each powder system was normalised with the AUC of ibuprofen-free powder to obtain the area ratio. The area ratio was plotted against mass fraction of ibuprofen, and the curvilinear data was fitted by third degree polynomial fitting, whose regression equation was back extrapolated to zero area ratio to yield dilution potential.
Results
The polynomial regression model was of the form: \(Area ratio={B}_{3}{x}^{3}+{B}_{2}{x}^{2}+{B}_{1}x\)+c, where x is ibuprofen mass fraction, and all the x coefficients and c are regression constants. The average predicted ibuprofen mass fractions (of the three independent tensile strength models) at zero area ratio were \(\approx 110.93\pm 1.02\) and \(\approx 116.02\pm 0.62\) for Tablettose100®-Starch-SSG composites and StarLac®, respectively.
Conclusion
From simplistic view point, dilution potential can reliability be computed from the simple Podzeck model relative to comparatively complex Shang and Pitt models.
Factors associated with polypharmacy and inappropriate medication use among older people: a longitudinal study of the Urban Health Centres Europe (UHCE)
Authors: Ye Lizhen , Yang-Huang Junwen , Franse Carmen Betsy , Rukavina Tomislav , Vasiljev Vanja , Mattace-Raso Francesco , Verma Arpana , Borrás Tamara Alhambra , Rentoumis Tasos , Raat Hein 
Published: May 2022

Abstract
Background: Polypharmacy and inappropriate medication use among older people is associated with adverse health consequences due to drug-drug interactions, drug-disease interactions, and adverse drug effects. The aim of this study is to assess the factors that are associated with polypharmacy and inappropriate medication use among community-dwelling older people in Europe.Method: This longitudinal study used baseline and follow-up data from 1791 participants of the Urban Health Center European project in five European countries. We studied factors in the domains (a) demographic characteristics, (b) lifestyle and nutrition, and (c) health and health care use, Hierarchical logistic regression models were used to examine the factors associated with polypharmacy and inappropriate medication.Results: Mean age was 79.6 years (SD±5.6 years); 60.8% were women; 45.2% had polypharmacy, and 41.8% had a high risk of inappropriate medication use. Participants who were women had higher odds of both having polypharmacy (OR=0.55;95%CI:0.42-0.72) and inappropriate medication use (OR=0.50;95%CI:0.39-0.65). Participants with a migration background (OR=1.67;95%CI:1.08-2.59), overweight (OR=1.37; 95%CI:1.04-1.79) and obesity (OR=1.78;95%CI:1.26-2.51) compared to ‘normal weight’, with lower physical HRQoL (OR=0.96, 95%CI:0.95-0.98), multi-morbidity (OR=3.73, 95%CI:2.18-6.37), frailty (OR=1.69, 95%CI:1.24-2.30), visited outpatient services (OR=1.77, 95%CI: 1.09-2.88) had higher odds of having polypharmacy. The associations with inappropriate medication use were similar.Conclusions: Multiple factors in the domains of demography, lifestyle, nutrition, and health care use are associated with polypharmacy and inappropriate medication use. These provide starting points to improve interventions to reduce polypharmacy and inappropriate medication use. In the meantime, health professionals can apply these insights to identify subgroups of patients are at a high risk of polypharmacy and inappropriate medication use. Trial registration: The intervention of the UHCE project was registered in the ISRCTN registry as ISRCTN52788952. The date of registration is 13/03/2017.
Quantitative imaging of magnetic nanoparticles in an unshielded environment using a large AC susceptibility array
Authors: Soares Guilherme , Pinto Leonardo , Liebl Maik , Biasotti Gabriel , Prospero Andre , Stoppa Erick , Bakuzis Andris , Baffa Oswaldo , Wiekhorst Frank , Miranda José Ricardo Arruda 
Published: May 2022

Abstract
Backgorund: Non-invasive magnetic imaging techniques are necessary to assist assist magnetic nanoparticles biomedical applications, mainly detecting their distribution inside the body. In Alternating Current Biosusceptometry (ACB), the magnetic nanoparticles magnetization response under an oscillating magnetic field, which is applied through an excitation coil, is detected with a balanced detection coil system. Results: We built a Multi-Channel ACB system (MC-ACB) containing nineteen pick-up coils and obtained 2D quantitative images of magnetic nanoparticles distributions by solving an inverse problem. We reconstructed the magnetic nanoparticles spatial distributions in a field of view of 14×14 cm² with a spatial resolution of 2.0 cm and sensitivity in the milligram scale. A correlation coefficient between quantitative reconstructed and nominal magnetic nanoparticles distributions above 0.6 was found for all measurements. Conclusion: Besides other interesting features such as sufficient large field of view dimension for mice and rat studies, portability, and the ability to assess the quantitative magnetic nanoparticles distributions in real-time, the MC-ACB system is a promising tool for quantitative imaging of magnetic nanoparticles distributions in real-time, offering an affordable setup for easy access in clinical or laboratory environments.
Conversion of a monospecific plantation into a mixed plantation: combining the light-demanding Araucaria angustifolia with the shade-tolerant Cabralea canjerana
Authors: Olguin Flavia Yesica , Moretti Ana Paula , Pinazo Martín Alcides , Goya Juan Francisco , Gortari Fermín , Bahima José Vera , Graciano Corina 
Published: May 2022

Abstract
The management of plantations of the critically endangered species Araucaria angustifolia (Bertol.) Kuntze can be an opportunity to mitigate the loss of local biodiversity and to contribute to wood production and Atlantic Forest conservation. Timber species that require canopy protection can be interplanted to convert monospecific stands in uneven-aged mixed plantations. We evaluated the establishment of Cabralea canjerana (Vell.) Mart under the canopy of A. angustifolia and initial competition in an uneven-aged mixed stand. In a 14-year-old stand we planted C. canjerana seedlings in three plots and other three were maintained as pure A. angustifolia plantation. We measured growth and competition in both species using competition indices and C. canjerana environmental and physiological traits to evaluate its acclimation during establishment. Five years after planting, seedling establishment was successful: plants reached a 9-meter height and 75% survived. A. angustifolia increment in diameter at breast height (DBH) was higher in mixed plots than in pure plots. The highest C. canjerana growth was observed with up to two A. angustifolia neighbours within a 5m radius. In other three A. angustifolia stands of different ages, C. canjerana establishment was successful. According to this evidence, it is possible to convert A. angustifolia even-aged stands to uneven-aged mixed stands with beneficial results for both species. In this way, a valuable native timber species that requires canopy protection during the first years can be planted outside the rainforest. This is the first report of a mixed plantation of two native Atlantic Forest timber species.
IL-1β/IL-1R1 signaling in the GIT regulates alpha-synuclein pathology and its propagation to the brain.
Authors: Haichen Niu , Muhammad Bilal , Li Haiying , Xue Senlin , Gao Ya , Xu Zhou , Fang Xiaoli , Ding Haohan , Gu Yunlu , Wu Fang , Geng Deqin 
Published: May 2022

Abstract
Accumulating evidence suggests that inflammatory bowel disease and misfolded alpha-synuclein (αsyn) of the gut are often found in Parkinson's disease (PD). However, the relationship between gastrointestinal inflammation with α-syn pathology and PD development is unknown. It is hypothesized that the α-syn pathology induced by gastrointestinal inflammation can begin in the enteric neural cells (ENS) of the gastrointestinal tract (GIT) and then propagate to the brain. To test this hypothesis, oral administration of rotenone (ROT) was used to induce GIT inflammation. PD-like responses were studied by histochemical and behavioral examinations in mice. Results show that the ROT treatments rendered macrophage activation, inflammatory mediator expression, and α-syn pathology in the GIT six weeks after the treatment. Pathological α-syn, namely pS129- α-syn, was found to be localized with IL-1R1 positive neural cells, which were transferred to the dorsal motor vagus (DMV) region via the vagus nerve. In addition, microglial activation and α-syn pathology (pS129- α-syn) in the ENS, DMV, and SN regions were observed in an interleukin-1β (IL-1β)/IL-1 receptor type I (IL-1R1) dependent. These results, therefore, reveal that inflammation of GIT can induce αsyn pathology and that IL-1β/IL-1R1-dependent propagation of α-syn via vagus nerve could lead to PD development.
Associations between serum vitamin D levels and visceral adipose tissue among adolescents: a cross-sectional observational study in NHANES 2011-2015
Authors: Li Yan-feng , Zheng Xiao , Gao Wen-lan , Tao Feng , Chen Yi 
Published: May 2022

Abstract
Backgrounds:
The incidence of obesity and vitamin D deficiency increases among adolescents. Improving our knowledge of the link between vitamin D and visceral adipose tissue (VAT) is essential for the health of adolescents. This study aimed to examine the connection between blood vitamin D levels and VAT mass among adolescents participating in the United States.
Methods
This is a cross-sectional study that used data from the 2011 to 2015 National Health and Nutrition Examination Survey (NHANES). The connection between blood vitamin D levels and VAT was investigated using weighted multiple linear regression models. Potential nonlinear relationships were explored using smooth curve fitting.
Results
An overall number of 3171 adolescents between the ages of 12 and 19 were recruited to take part in this research. Vitamin D levels were shown to be inversely linked with VAT in the full-adjusted model (β= -0.38, 95% CI: -0.53 to -0.23). When subgroup analysis was stratified by gender, this negative connection remainder in the girls' group (β= -0.44, 95% CI: -0.65 to -0.22), but not in the boys' group (β= -0.07, 95% CI: -0.26 to 0.12). When subgroup analysis was stratified by race, this negative connection remained in the Mexican American group (β= -0.63, 95% CI: -1.04 to -0.21), and the non-Hispanic White group (β= -0.35, 95% CI: -0.62 to -0.08), but not in the other groups.
Conclusions
In this investigation, it was shown that serum vitamin D levels negatively correlated with VAT in girls, the Mexican American and non-Hispanic White in adolescents.