bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sequencing and Structural Characteristic Analysis of Mitochondrial Genome in Zhijin White Goose(Anser cygnoides)
Authors: Guo Zhi-Li , Zhang Yong , Yang Hong , Wang Tian-Song , Wang Wei-Wei , Zeng Shan-Shan , Guo Yong , Ye Li , Du Ao , Wang Zhi-Wei , Zeng Su-Mei , Yuan Jian , Wang Lei 
Published: April 2022

Abstract
Zhijin White Goose has the characteristics of fast growth, good fattening performance, fresh and high-quality meat, rough feeding resistance, and strong disease resistance. It is a precious white goose resource unique to Guizhou Province, China. However, the number of Zhijin White Geese is decreasing endangered state. The purpose of this study to establish a breed conservation standard of Zhijin White Goose by using mitochondrial genome(mtGenome), and preliminarily analyze the mtGenome. In this study, the mtGenome sequence (GenBank MZ427898) of Zhijin White Goose was obtained by the high-throughput sequencing method. The mtGenome differences between the Zhijin White Goose and other different geese breeds were analyzed, and determining the phylogenetic position of the Zhijin White Goose in Anseriformes. The structural characteristics of some sequences and the secondary structural characteristics of RNA were analyzed, which in order to provide some theoretical reference for the follow-up functional research. The results demonstrated that the length was 16 739 bp, and the base composition was A (30.22%), G (15.08%), C (32.23%), and T (22.47%). It contained 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, and 1 D-loop region. The AT content of the mtGenome and PCGs in Zhijin White Goose was 51.58% and 52.69%, respectively, which showed AT preference. The 22 tRNAs, except tRNA-Ser (AGY), form a typical clover structure. Phylogenetic tree analysis based on the mtGenome revealed that the Zhijin White Goose is closely related to the Swan Goose and Chinese Goose and is distantly related to breeds outside China. In conclusion, the mtGenome can be used as a standard to distinguish Zhijin White Goose from other goose breeds, and it can be used as a breeds, candidate identification criterion for Zhijin White Goose.
Optimization of Mechanical Design Bladeless Wind Turbine for Electricity Fulfilment in Nusa Tenggara Timur, Indonesia
Authors: Ramadhany Muhammad Farhan , Kusuma Theo Aden , Chelsie Yessika Natalia , Adi Gandha Satria 
Published: April 2022

Abstract
The current government's goal is the realization of an even distribution of the electrification ratio in Indonesia. However, in 2018 the electrification ratio in Nusa Tenggara Timur (NTT) only reached 62.07%. One of the solutions offered is the implementation of a Bladeless Wind Turbine (BWT). BWT is a type of wind power plant that can work optimally in areas with low wind speeds, 3 to 8 m/s, which is still above the NTT average wind speed, 2.3 m/s. This study aims to optimize the mechanical design of BWT with shape and size parameters based on the manipulation of the coefficient of friction through computational fluid dynamic simulations that can work optimally according to wind speed in NTT. In this study, 3 design variations were used, namely Initial Design, Modified Design 1, and Modified Design 2. Based on the research that has been done, MD2 has better results than MD1 and ID, and it can be said that MD2 can work optimally in wind speed areas. low. So MD2 is a mechanical design model that is very suitable to be applied in NTT and is expected to be a recommendation in making masts for BWT-based wind power plants in Indonesia.
Implication of Inflammation on Coxsackie-Adenovirus Receptor Expression on Cardiomyocytes and the Role of Platelets in Patients with Dilated Cardiomyopathy
Authors: Rimskaya Elena M. , Buryachkovskaya Liudmila I. , Chumachenko Petr V. , Narusov Oleg Yu. , Mironova Natalia A. , Tereschenko Sergei N. , Golitsyn Sergei P. , Othman Maha 
Published: April 2022

Abstract
BackgroundCoxsackie-adenovirus receptor (CAR) is involved in the pathogenesis of inflammatory dilated cardiomyopathy (DCM). We aimed to examine the relationship of CAR expression on platelets and cardiomyocytes with virus persistence, local and systemic inflammation and platelet activity in patients with DCM. MethodsEndomyocardial biopsy (EMB) samples of 38 patients (mean age 39.5±11.3 years, 20 male) with DCM were analyzed for CAR expression, local inflammation grade by immunohistochemistry and virus persistence by real-time PCR. Platelet morphology was analyzed in all patients and 30 healthy subjects (HS) using scanning electron microscopy, platelet activity by light transmission aggregation, and CAR persistence by immunofluorescence. Platelets of 20 patients were analyzed for cytomegalovirus and herpes simplex virus 1-2 by immunofluorescence. Serum levels of tumor necrosis factor alpha (TNF α) and Interleukin-6 were assessed using ELISA in all studied subjects.Results:CAR expression in EMB samples was related to the heart failure functional class and the level of IL-6. Platelets from DCM patients showed enhanced spontaneous and ADP induced aggregation. Platelets’ CAR expression was >4 fold higher in DCM than HS and was observed predominantly at sites of intercellular communications in microaggregates and leukocyte-platelet aggregates. CAR-positive patients showed significantly higher TNF-α and IL-6 serum levels in CAR-negative patients. Platelets of 6 (30%) DCM patients revealed the mature cytomegalovirus and herpes simplex viruses particles. ConclusionTight junction protein CAR may serve as a docking pin creating a new type of contact structure that could be responsible for signaling between neighboring cells in pathological conditions.
The effect of bacteria inoculation on the lignocellulose degradation and the microbial properties during cow dung composting
Authors: Zhou Liuyan , Yang Xinping , Xie Yuqing , Wang Xiaowu , Dai Jinping , Wang Zhifang , Zhang Huitao , Feng Lei 
Published: April 2022

Abstract
The goal of this work was to explore the potential effects of bacteria inoculation on the lignocellulose degradation and the underlying microbial mechanism during cow dung composting. The results suggested that bacteria inoculation better-accelerated temperature rose and increased thermophilic phase. Compared to the control (without inoculation, CK), the cellulose, hemicellulose, and lignin degradation rates of treated group (EG) increased from 53.3% to 70.0%, 50.2% to 61.3% and 46.4% to 60.0%, respectively. The microbial community structure and diversity were obviously changed by inoculation. Moreover, inoculation contributed to modulate the key compost microbial functional populations linking to the degradation of lignocelluloses by stamp analysis. Correlation matrix analysis indicated that the expression of bacterial lingo-cellulolytic enzymes were closely related to the key microbial functional populations. Overall, the results confirmed the importance of bacteria inoculation and have important implication for promoting the efficiency and quality of cow dung compost.
Reversal of fetal heart block in antibody-positive mother after hydroxychloroquine and dexamethasone
Authors: Hoda Mehar , Scott William , Sharma Kavita , Duryea Elaine , Ikemba Catherine 
Published: April 2022

Abstract
Maternal autoantibody related complete heart block in the fetus is considered irreversible. During prenatal care for a 25 year old nulliparous Hispanic woman with newly diagnosed nephrotic range proteinuria and positive anti-nuclear antigen antibody, complete fetal heart block with a ventricular rate of 60 beats per minute was detected on a fetal echocardiogram at 28 weeks gestation. A small pericardial effusion and ascites were noted consistent with fetal hydrops. Dexamethasone and hydroxychloroquine were initiated. Fetal rhythm improved to Mobitz type 1 second-degree heart block, with a ventricular rate of 91 beats per minute. The fetus was born prematurely at 34 weeks gestation with second-degree heart block which improved to first-degree heart block prior to hospital discharge. First-degree heart block persisted at 2 years of age with a P-R interval of 185 milliseconds. Transplacental treatment with dexamethasone and hydroxychloroquine were associated with sustained reversal of complete heart block to sinus rhythm.
The effect of “Fishery-PV Integration” on Penaeus monodon culture and Micro-ecological environment
Authors: Sun Chengbo , Liao Minze , Long Xinxin , He Zihao , Zhao Jichen , Chen Xieyan , Zhu Dongwenjun 
Published: April 2022

Abstract
Background: “Fishery-photovoltaic (PV) Integration” is a novel aquaculture model which aims to combine green energy with modern fisheries to improve socioeconomic benefits while ensuring aquaculture production and electricity consumption. To determine the practicability of the “Fishery-PV Integration” model, an experiment was conducted with Penaeus monodon as a model, in which the growth rates of shrimp, water quality conditions, and the micro-ecological environment were studied. Results: After 105 days of rearing, P. monodon (initial weight of 0.006 ± 0.001 g) reached an average body weight of 11.69 ± 0.92 g. The water quality was stable (C(NH4+) ≤ 1.57 mg/L), and the shrimps were healthy. It was determined via metagenomics and 16S rRNA gene sequencing that the dominant phyla present in shrimp intestines, water, and effluent were Proteobacteria, Actinobacteriota, Bacteroidota, and Verrucomicrobiota. In addition, a large number of nitrogen-fixing bacteria in the “Fishery-PV Integration” pond, which were helpful to maintain the stability of the water quality. The relative abundance of Vibrio as a common opportunistic pathogen for shrimp was relatively low, which is beneficial to promote the healthy growth of shrimp. In addition to maintaining water quality stability, microorganisms played an essential role in regulating intestinal amino acid metabolism, carbohydrate metabolism, and energy metabolism. In the water and effluent, a high proportion of ABC transporters and quorum sensing play an important role in mitigating environmental toxins and regulating the physiological and metabolic activities of microbial communities. Notably, only 123 antibiotic resistance genes (ARGs) were identified within the “Fishery-PV Integration” pond. This result is significantly lower than the numbers found in other studies, indicating that shrimp cultured in the “Fishery-PV Integration” pond had a higher level of food safety. Conclusions: This paper was the first reported the “Fishery-PV Integration” culture mode and preliminarily reveal the characteristics of the microflora and ARGs distribution in a “Fishery-PV Integration” pond. The results show that “Fishery-PV Integration” farming is a sustainable, green, and safe culture method.
Quantitative assessment of eye movements using a binocular paradigm: comparison among amblyopic, cured amblyopic and normal children
Authors: Zhou Yulian , Bian Hewei , Yu Xiaobin , Wen Wen , Zhao Chen 
Published: April 2022

Abstract
Background: To investigate the fixation and saccade functions in children with amblyopia and cured amblyopia by a binocular eye-tracking paradigm.Methods: Eye movements of 135 pediatric subjects, including 45 amblyopic children, 45 cured amblyopic children and 45 age-similar normal controls, were recorded under binocular viewing. The deviation (degree) between fixation positions and the visual targets along horizontal or vertical axis was recorded as the mean from both eyes. Deviations were compared across three groups and between each two groups.Results: Main outcome measures included deviations along horizontal and vertical axes in the circular fixation test (Fix-X, Fix-Y) and horizontal saccade test (Sac-X, Sac-Y). All four deviations were significantly larger in the amblyopia group compared to the other two groups, indicating fixation and saccade deficits. However, there was no significant difference in deviations between cured amblyopic children and normal controls. In the amblyopia group, four deviations were similar across subgroups of varying amblyopia severity. Larger deviations were related to younger age and poor visual acuity overall.Conclusion: Amblyopic children have poor fixation and saccade functions, while cured amblyopic children seem to restore the functions. This paradigm provides a promising technique to evaluate and follow up the functional recovery.
Evaluation of Choroidal thickness in patients with unilateral ambylopia using spectral domain optical coherence tomography (SD-OCT) with quadrant wise analysis
Authors: Goyal Ashima , Arora Priyanka , Mahapatra Madhushmita , verma Ritesh 
Published: April 2022

Abstract
Aims and objectives- To determine the choroidal thickness (mainly subfoveal) in the eyes of subjects with anisometropic amblyopia and strabismic amblyopia and to compare the choroidal thickness with that of fellow eyes (control group).Material and Methods- In this prospective study, 140 eyes of 70 patients were included (age 5-40 years). Each patient underwent a detailed ocular examination which included visual acuity, intra ocular pressure, keratometry, refraction, axial length and choroidal thickness measurement. The choroidal thickness (CT) was measured using enhanced depth imaging (EDI) mode in spectral domain optical coherence tomography (SD-OCT) at subfoveal, o.5 mm, 1mm, 1.5mm in both nasal and temporal quadrants.Results- The mean sub-foveal CT was 341.73±60.39 µm in amblyopic eyes and 314.77±48.12 µm in control eyes. The CT was significantly thicker in anisometropic amblyopic eyes than fellow eyes (p=0.00), whereas in strabismic amblyopic eyes it was not significantly thicker (p=0.064). Conclusion- Significant choroidal thickening was observed in subjects with amblyopia, which may contribute to the amblyopia pathogenesis. These changes were more pronounced in patients with anisometropic amblyopia than strabismic amblyopia.
MAC mediates mammary duct epithelial cell injury in Plasma cell mastitis and Granulomatous mastitis
Authors: Li：master Xiaoqiang , Zhang Haojie , Ding Peng-peng , Zhang Xiang-sheng , Wang Xi-chao , Sun Di-wen , Bu Qing-ao 
Published: April 2022

Abstract
Plasma cell mastitis (PCM) and granulomatous mastitis (GM) are common inflammatory nonbacterial mastitis (NBM). However, the pathogenesis of NBM is still unclear.Methods. In this study, we statistically analyzed the pathological features of PCM and GM using pathological HE staining and tissue transmission electron microscopy. The levels of MAC (C5b-9n), P-selectin, E-selectin, and ICAM-1 were detected through IHC, WB, ELISA, and qPCR. The expression level and location of MAC were observed by tissue immunological electron microscopy. In addition, exosomes were isolated from tissues, identified using transmission electron microscopy, and the densities were detected by Nano-FCM. Finally, the expression intensity of MAC in exosomes was detected by flow cytometry and immunoelectron microscopy.Results. The damage and apoptosis of mammary duct epithelial cells are the common pathological features of PCM and GM. MAC is primarily located in the cell membrane of mammary ductal epithelial cells and is significantly expressed in PCM and GM. The density of exosomes in PCM and GM tissues was elevated, and MAC was highly expressed in exosomes. In addition, the expression of P-selectin, E-selectin, and ICAM-1 in PCM and GM was significantly higher than in the normal group.Conclusion. We found severe damage of the mammary duct epithelial cells in PCM and GM tissues, which was verified by relevant pathological methods. Earlier studies demonstrated that MAC is highly expressed in PCM and GM tissues and exosomes seem to play a very important role in the understanding of MAC. Furthermore, MAC is involved in inflammatory infiltration and lesion of mammary duct epithelial cells upregulated by P-selectin, E-selectin, and ICAM-1. These findings provide new insights into PCM and GM molecular mechanisms with novel therapeutic advances.
Metagenomic insight into shifts of methane-cycling communities following pasture conversion in the Amazon basin
Authors: Alvarez Dasiel Obregon , de Souza Leandro Fonseca , Mendes Lucas William , de Moraes Moacir Tuzzin , Tosi Micaela , Venturini Andressa Monteiro , Meyer Kyle M. , de Camargo Plínio Barbosa , Bohannan Brendan J.M. , Rodrigues Jorge L. Mazza , Dunfield Kari E. , Tsai Siu Mui 
Published: April 2022

Abstract
Background: Deforestation threatens the integrity of the Amazon biome and the ecosystem services it provides. Most of this deforested land is converted to pastures for cattle raising. Early studies revealed that forest-to-pasture conversion alters the flux of methane gas (CH4) in Amazonian soils, driving a switch from acting as a sink to a source of atmospheric CH4. We sought to better understand this phenomenon by investigating the soil microbial metagenomes, focusing on the taxonomic and functional structure of methane-cycling communities.
Results: Metagenomic data were combined with in situ measurements of CH4 fluxes, and measurements of soil edaphic factors. We found a significantly higher abundance of methanogens and reduced methanotrophs in pasture soils. As inferred by co-occurrence networks, these microorganisms seem to be less interconnected within the soil microbiota in pasture soils. Metabolic traits were also different between land uses, with increased hydrogenotrophic and methylotrophic pathways of methanogenesis in pasture soils. Similarly, methanotrophs harboring the soluble form of methane monooxygenase enzyme (sMMO) were depleted in pasture soils. Redundancy analysis and multimodel inference revealed that the shift in methane-cycling communities in pasture soils was associated with higher soil compaction, pH, organic matter, and micronutrients.
Conclusions: These results provide new knowledge about the effect of forest-to-pasture conversion on the community traits of methane-cycling microorganisms in the Amazon region, resulting in increased abundance and functional potential of methanogens in pastures, and thus increasing the emission of CH4 in deforested soils.
Keywords: land-use change, methanogens, methanotrophs, soil properties, multimodel inference
Treated domestic effluents for non-potable reuse: microbial risk assessment and economic viability
Authors: Santos Luciene da Silva , Souza Hugo Henrique de Simone , Amoah Isaac Dennis , Magri Maria Elisa , Ide Carlos Nobuyoshi , Paulo Paula Loureiro 
Published: April 2022

Abstract
Although water quality can be ensured for various pollutant removal processes, concerns regarding microbial pathogens can set very high-quality standards for treated wastewater rendering the practice financially unfeasible. In this study, we applied quantitative microbial risk assessment and net present value methodologies to evaluate the economic viability and safety of wastewater treatment to produce water for reuse. Under the evaluated conditions, even though applying tertiary treatments (such as chlorination, ozonation, or UV radiation) for non-potable reuse purposes presents higher investment costs, it could offer a faster return on investment as a result of the greater income derived from the sale of the treated wastewater, and consequently, reduce the risk of exposure to pathogens. An economic viability analysis revealed payback periods of three to over 15 years for investment in the universalisation of sanitation services in the selected municipalities, wherein the most feasible scenarios were those in which water reuse was carried out at its maximum potential. We evaluated the reuse potential of treated wastewater in terms of reduced concentrations of Escherichia coli and observed that effluents treated by six wastewater treatment plant configurations (involving secondary treatment processes) could be used for restricted crop irrigation, with disability-adjusted life years (DALYs) of 9.20E03 ± 4.59E05 related to the risk of infection to the farmers. The application of additional disinfection procedures indicated that treated wastewater could be reused for unrestricted and restricted urban and agricultural uses, with corresponding DALYs of 1.35E05 ± 6.73E08 for the same group.
The robust-reliable decision for selecting benchmark automotive platforms based on the combination of humane judgment simulation and KDD techniques
Authors: Saghari Asad , Hosseinimehr Masoud , Rahmani Shima , Budinská Ivana 
Published: April 2022

Abstract
In this study, we address robust-reliable decision-making approach to select benchmark platforms to develop the automotive family. Activities studied included selecting the appropriate decision-making method, simulating possible scenarios to determine the relative importance of attributes by experts and stakeholders, determining the decision space and valuing attributes based on databases and Knowledge discovery in databases (KDD) techniques, statistical analysis and sensitivity assessment. The robust-reliable decision is made using the Simple Additive Weighting (SAW) method for 6223 unique cases of expert judgments and stakeholder expectations. The database used to determine decision space and attributes values included 546 automobiles designed in 11 different segments based on 34 platforms. This has led to the reliable selection of five benchmark platforms with the highest level of desirability in terms of defined attributes and the greatest robustness to uncertainties in the expert judgments and the level of expectations of stakeholders.
High-throughput sequencing analysis of differentially expressed microRNAs associated with SREBP1 in differentiated thyroid carcinoma
Authors: Zhou Jiating , Liu Jianqiu , Zhang Ke , Lv Jing , Peng Xiaowei , Liu Jie , Yin Jiye , Li Zhi 
Published: April 2022

Abstract
MicroRNAs (miRNAs) interact with genes posttranscriptionally and play important roles in progression and prognosis in multiple cancers. Sterol regulatory element-binding protein 1 (SREBP1) is an important lipid metabolism regulatory gene. The aim of the present study was to analyze the profiles of miRNAs that associated with SREBP1 expression in differentiated thyroid carcinoma (DTC). In the present study, a high-throughput small RNA sequencing (miRNA-Seq) method was used to investigate differences in miRNA profiling with versus without interference in the expression of SREBP1 via small interfering RNA. Real-time qPCR (qRT-PCR) was performed to confirm the results. A total of 1393 conserved and 84 novel miRNAs were successfully discovered. Among them, 27 (11 upregulated and 16 downregulated) differentially expressed miRNAs were significantly altered in BCPAP cells following SREBF1 interference of two fragments compared with the control in two batch. Hsa-miR-941, hsa-miR-27a-5p, hsa-miR-29a-3p, hsa-miR-100-5p, and hsa-miR-21-3p were selected for validation by qRT-PCR. The qRT-PCR results were consistent with the sequencing data. GO enrichment showed that the predicted targets of these miRNAs were mainly involved in the regulation of system development, metabolism and protein binding cellular processes, and metabolic processes. KEGG pathways analysis showed that predicted target genes were involved in several signaling pathways, including the Ras, MAPK, insulin, thyroid hormone and metabolic pathway signaling pathways. Our findings suggest that differentially expressed miRNAs and their target genes may play an important role in the progression and prognosis of DTC that related with SREBP1 expression.