bluuebunny/crossref_metadata_2025_split huggingface dataset:
C1QTNF6 is a Prognostic Biomarker and Related to Immune Infiltration and Drug Sensitivity: A Pan-Cancer Analysis
Authors: Liu Wei , Zhang Jian , Xie Tao , Huang Xiaoting , Wang Baiyao , Tian Yunhong , Yuan Yawei 
Published: March 2022

Background: The discovery of reliable cancer biomarkers could tune a diagnosis and improve the way patients are treated. However, many cancers lack robust biomarkers. C1QTNF6 has been preliminarily elucidated for its role in some tumors. However, no pan-cancer analysis has been performed to comprehensively explore the value of C1QTNF6.Methods: Data from the TCGA database, GTEx database stored in the USUC Xena were used for analyzing the profiles of C1QTNF6 expression in normal and tumor tissues in pan-cancer. Subsequently, the gene alteration rates of C1QTNF6 were acquired on the online web cBioportal. With the aid of the TCGA data, the association between C1QTNF6 mRNA expression and copy number alterations (CNA) and methylation was determined. Survival analyses of C1QTNF6 were carried out. Moreover, the tumor biological and immunological characteristics of C1QTNF6 were clarified in the forms of the correlation between C1QTNF6 expression and hallmark Pathway scores in MsigDB database, immune cell infiltration, immune-related genes. We conducted a GSEA of C1QTNF6 to illustrate its potential biological functions. In addition, GDSC2 data with 198 drugs were adopted to explore drug sensitivity with the change of C1QTNF6 expression.Result:C1QTNF6 was overexpressed in many types of cancer, Survival analysis showed that C1QTNF6 independently served as a prognostic indicator for poor survival in many tumors. Besides, we also identified a positive correlation between C1QTNF6 and cancer hallmark pathway score, tumor microenvironment related pathways score (TMEp score), and immune characteristic. In terms of drug sensitivity analysis, we found higher expression level of C1QTNF6 predicts a high IC50 value for most of 198 drugs which predicts drug resistance.Conclusions: Our study provides a new biological marker for pan-cancer, which is beneficial to the diagnosis and treatment of cancer, which bring a new therapeutic target for tumors.
Investigating the Implications of CFTR Exon Skipping Using a Cftr Exon 9 Deleted Mouse Model
Authors: Martinovich Kelly M. , Kicic Anthony , Stick Stephen M. , Johnsen Russell D. , Fletcher Sue , Wilton Steve D. 
Published: March 2022

Introduction: Severity and disease progression in people with Cystic Fibrosis (CF) is typically dependent on their genotype. One potential therapeutic strategy for people with specific mutations is exon skipping with antisense oligonucleotides (AO). CFTR exon 9 is an in-frame exon and hence the exclusion of this exon would excise only 31 amino acids but not alter the reading frame of the remaining mRNA. Splice mutations 1209 + 1 G &gt; C and 1209 + 2 T &gt; G were documented to cause CFTR exon 9 skipping and these variants were reported to manifest as a milder CF disease, therefore exon 9 skipping could be beneficial for people with class I mutations that affect exon 9 such as p.Trp401X. While the impact of exon 9 skipping on gene expression and cellular pathways can be studied in cells in vitro, trace amount of full-length normal or mutated material could confound the evaluation. To overcome this limitation, the impact of CFTR exon 9 skipping on disease phenotype and severity is more effectively evaluated in a small animal model. It was hypothesised that antisense oligonucleotide-mediated skipping this particular exon could result in a “mild mouse CF phenotype”.Methods:Cftr exon 9 deleted mice were generated using homologous recombination. Survival of homozygous (CftrΔ9/Δ9) and heterozygous (CftrΔ9/+) mice was compared to that of other CF mouse models, and lung and intestinal organ histology examined for any pathologies. Primary airway epithelial cells (pAECs) were harvested from CftrΔ9/Δ9 mice and cultured at the Air Liquid Interface for CFTR functional assessment using Ussing Chamber analysis.Results: A CftrΔ9/Δ9 mouse model presented with intestinal obstructions, and at time of weaning (21 days). CftrΔ9/Δ9 mice had a survival rate of 83% that dropped to 38% by day 50. Histological sections of the small intestine from CftrΔ9/Δ9 mice showed more goblet cells and mucus accumulation than samples from the CftrΔ9/+ littermates. Airway epithelial cell cultures established from CftrΔ9/Δ9 mice were not responsive to forskolin stimulation.Summary: The effect of Cftr exon 9 deletion on Cftr function was assessed and it was determined that the encoded Cftr isoform did not result in a milder “mouse CF disease phenotype,” suggesting that Cftr exon 9 is not dispensable, although further investigation in human CF pAECs would be required to confirm this observation.
Therapeutic Targeting of Endosome and Mitochondrial Reactive Oxygen Species Protects Mice From Influenza Virus Morbidity
Authors: To Eunice E. , Erlich Jonathan R. , Liong Felicia , Liong Stella , Luong Raymond , Oseghale Osezua , Miles Mark A. , Papagianis Paris C. , Quinn Kylie M. , Bozinovski Steven , Vlahos Ross , Brooks Robert D. , O’Leary John J. , Brooks Doug A. , Selemidis Stavros 
Published: March 2022

There is an urgent need to develop effective therapeutic strategies including immunomodulators to combat influenza A virus (IAV) infection. Influenza A viruses increase ROS production, which suppress anti-viral responses and contribute to pathological inflammation and morbidity. Two major cellular sites of ROS production are endosomes via the NOX2-oxidase enzyme and the electron transport chain in mitochondria. Here we examined the effect of administration of Cgp91ds-TAT, an endosome-targeted NOX2 oxidase inhibitor, in combination with mitoTEMPO, a mitochondrial ROS scavenger and compared it to monotherapy treatment during an established IAV infection. Mice were infected with IAV (Hkx31 strain; 104PFU/mouse) and 24 h post infection were treated with Cgp91ds-TAT (0.2 mg/kg), mitoTEMPO (100 μg) or with a combination of these inhibitors [Cgp91ds-TAT (0.2 mg/kg)/mitoTEMPO (100 μg)] intranasally every day for up to 2 days post infection (pi). Mice were euthanized on Days 3 or 6 post infection for analyses of disease severity. A combination of Cgp91ds-TAT and mitoTEMPO treatment was more effective than the ROS inhibitors alone at reducing airway and neutrophilic inflammation, bodyweight loss, lung oedema and improved the lung pathology with a reduction in alveolitis following IAV infection. Dual ROS inhibition also caused a significant elevation in Type I IFN expression at the early phase of infection (day 3 pi), however, this response was suppressed at the later phase of infection (day 6 pi). Furthermore, combined treatment with Cgp91ds-TAT and mitoTEMPO resulted in an increase in IAV-specific CD8+ T cells in the lungs. In conclusion, this study demonstrates that the reduction of ROS production in two major subcellular sites, i.e. endosomes and mitochondria, by intranasal delivery of a combination of Cgp91ds-TAT and mitoTEMPO, suppresses the severity of influenza infection and highlights a novel immunomodulatory approach for IAV disease management.
Long-Term Posttraumatic Growth in Victims of Terrorism in Spain
Authors: Fausor Rocío , Sanz Jesús , Navarro-McCarthy Ashley , Gesteira Clara , Morán Noelia , Cobos-Redondo Beatriz , Altungy Pedro , Marqueses José M. S. , Sanz-García Ana , García-Vera María P. 
Published: March 2022

BackgroundScientific literature on posttraumatic growth (PTG) after terrorist attacks has primarily focused on persons who had not been directly exposed to terrorist attacks or persons who had been directly exposed to them, but who were assessed few months or years after the attacks.MethodsWe examined long-term PTG in 210 adults directly exposed to terrorist attacks in Spain a mean of 29.6 years after the attacks (range: 2–47 years). The participants had been injured by a terrorist attack (38.6%) or were first-degree relatives of people who had been killed or injured by a terrorist attack (41.4% and 20%, respectively). They completed diagnostic measures of emotional disorders and measures of PTSD and depression symptomatology, optimism, and PTG.ResultsMultiple regression analyses revealed gender differences (women reported higher levels of PTG than did men) and a positive linear relationship between PTG and cumulative trauma after the terrorist attack. Some PTG dimensions were significantly associated with PTSD symptomatology, these associations being linear, not curvilinear. However, PTG was not associated with depression symptomatology, diagnosis of emotional disorders, age, elapsed time since the attack, or optimism. In comparison with survivors assessed 18 years after the 1995 Oklahoma City bombing, Spanish victims of terrorism showed higher levels of appreciation of life, but lower levels of relating to others and spiritual change.ConclusionThe findings underscore the influence of gender on PTG and provide support to the hypothesis that some emotional distress may be a necessary condition of PTG. Future studies on PTG after terrorist attacks should take into consideration the characteristics of the terrorist attack itself and the contexts of violence and threat in which it occurred. The political, social, and cultural characteristics of the community affected by it and the profile and characteristics of other traumatic events suffered after the attack should also be taken into account in further research.
Immune-Related lncRNAs Pairs to Construct a Novel Signature for Predicting Prognosis in Gastric Cancer
Authors: Bao Tianshang , Wang Zeyu , Xu Jia 
Published: March 2022

BackgroundImmune-related long non-coding RNAs (irlncRNAs) appear valuable in predicting prognosis in patients with cancer. In this study, we used a fresh modeling algorithm to construct irlncRNAs signature and then assessed its predictive value for prognosis, tumor immune infiltration, and chemotherapy efficacy in gastric cancer (GC) patients.Materials and MethodsThe raw transcriptome data were extracted from the Cancer Genome Atlas (TCGA). Patients were randomly divided into the training and testing cohort. irlncRNAs were identified through co-expression analysis, after which differentially expressed irlncRNA (DEirlncRNA) pairs were identified. Next, we developed a model to distinguish between high- or low-risk groups in GC patients through univariate and LASSO regression analyses. A ROC curve was used to verify this model. After subgrouping patients according to the median risk score, we investigated the connection between the risk score of GC and clinicopathological characteristics. Functional enrichment analysis was also performed.ResultsWe find that the results indicate that immune-related lncRNA signaling has essential value in predicting prognosis, and it may be potential to measure the Efficacy for immunotherapy. This feature may be a guide to the selection of GC immunotherapy.ConclusionOur data revealed that immune-related lncRNA signaling had essential value in predicting prognosis, and it may be potentially used to measure the efficacy for immunotherapy. This feature may also be used to guide the selection of GC immunotherapy.
Paracetamol (Acetaminophen) and its Effect on the Developing Mouse Brain
Authors: Philippot Gaëtan , Hosseini Kimia , Yakub Armine , Mhajar Yasser , Hamid Mariam , Buratovic Sonja , Fredriksson Robert 
Published: March 2022

Paracetamol, or acetaminophen (AAP), is the most commonly used analgesic during pregnancy and early life. While therapeutic doses of AAP are considered harmless during these periods, recent findings in both humans and rodents suggest a link between developmental exposure to AAP and behavioral consequences later in life. The aim of this study is to evaluate the impact of neonatal exposure to clinically relevant doses of AAP on adult spontaneous behavior, habituation, memory, learning, and cognitive flexibility later in life using a mouse model. Markers of oxidative stress, axon outgrowth, and glutamatergic transmission were also investigated in the hippocampus during the first 24 h after exposure. In addition, potential long-term effects on synaptic density in the hippocampus have been investigated. In a home cage setting, mice neonatally exposed to AAP (30 + 30 mg/kg, 4 h apart) on postnatal day 10 displayed altered spontaneous behavior and changed habituation patterns later in life compared to controls. These mice also displayed reduced memory, learning and cognitive flexibility compared to control animals in the Morris water maze. An increase of markers for oxidative stress was observed in the hippocampus 6 h after AAP exposure. As AAP is the first choice treatment for pain and/or fever during pregnancy and early life, these results may be of great importance for risk assessment. Here we show that AAP can have persistent negative effects on brain development and suggest that AAP, despite the relatively low doses, is capable to induce acute oxidative stress in the hippocampus.
Morphological and Molecular Description of Sarcocystis myodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania
Authors: Rudaitytė-Lukošienė Eglė , Jasiulionis Marius , Balčiauskas Linas , Prakas Petras , Stirkė Vitalijus , Butkauskas Dalius 
Published: March 2022

Numerous rodent species have been broadly examined for Sarcocystis parasites. Nevertheless, recent investigations on Sarcocystis spp. in voles are lacking. As many as 45 bank voles (Clethrionomys glareolus) captured in several locations in Lithuania were examined in the present study. Based on morphological, genetic, and phylogenetic results, sarcocysts detected in one bank vole were described as Sarcocystis myodes n. sp. Using light microscopy analysis, the observed sarcocysts were ribbon-shaped, 6000–3000 × 70–220 µm in size. Sarcocysts were characterized by a relatively thin (about 1 μm) and apparently smooth cyst wall. The lancet-shaped bradyzoites were 9.6–12.0 × 3.1–4.6 μm in size. By transmission electron microscopy, the sarcocyst wall was up to 1 μm thick, parasitophorous vacuolar membrane had small knob-like blebs. Based on 18S rDNA, 28S rDNA, cox1, rpoB, and ITS1 loci, S. myodes showed highest similarity with S. ratti from the black rat (Rattus rattus). According to phylogenetic placement, S. myodes was most closely related to Sarcocystis spp. that employ predatory mammals as their definitive hosts. Morphologically, sarcocysts of S. myodes have similar features to those of S. cernae, S. dirumpens, and S. montanaensis described in voles, however, they use birds of prey or snakes as their definitive hosts.
Apoptotic And Antiangiogenic Effects of Noscapine In Human Lung Cancer (A549) Cells
Authors: İSLAM İzzet , KARAGÖZ Alper , AYTUĞ Hande , BULDUK İbrahim , KARABAĞ ÇOBAN Doç. Dr. Funda 
Published: March 2022

Objective: Lung cancer, one of the most important health problems today, is the most common type of cancer that causes death in both women and men.
Although A549 lung cells originating from human alveolar carcinoma cells match the type II alveolar cell phenotype, it also has many characteristics that human primary alveolar epithelial cells have.
Noscapine consists of the components in the poppy somniferum (opium). It was first isolated from Papaver somniferum (opium) in 1817. It consists of the most abundant opioids found in the opium plant (up to 10% of the total composition) after morphine. It is also known as Narcotine, Nectodon, Nospen, Anarcotine and (archaic) Opiane, and S is an (-) isomer with R stereochemistry (S stereochemistry in phthalid-carbon and R in isoquinoline-carbon). Noscapine is structurally and chemically different from other opium alkaloids such as morphine, codeine, thebaine, papaverine and narcein.The aim of the study the ınvestıgatıon of the apoptotıc and antıangıogenıc effects of noscapine ın human lung cancer (A549) cells.
Materials and Methods: In the study, concentrations of 10 ppm, 15 ppm, 20 ppm, 25 ppm, 40 ppm, 50 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 90 ppm and 100 ppm were used in the proliferation experiment proportion of vitality values. It was observed that it decreased and the LD50 value was determined as 40 ppm. Statistical analysis used: Statistical analysis of the analyzes was performed using SPSS Statistics 20,0 program.
Results: VEGF values decreased at 20 ppm, 40 ppm and 80 ppm noscapine concentrations compared to the control group. There was a significant decrease in PARP values at 80 ppm.
Conclusions: As a result of the findings; It is thought that concentrations of noscapine 10 ppm, 20 ppm and 40 ppm can reduce angiogenesis and prevent metastasis by lowering VEGF levels. PARP levels decreased at all noscapine concentrations but the most significant difference was seen at 80 ppm noscapine concentration.
Modern Representation of Periventricular Leukomalacia Genesis in Premature Newborns
Authors: Жевнеронок И.В. , Шалькевич Л.В. , Лунь А.В. 
Published: March 2022

В статье представлены данные о механизмах возникновения, факторах риска, частоте встречаемости перивентрикулярной лейкомаляции (ПВЛ) у недоношенных новорожденных. Приведены классификационные признаки различных типов ПВЛ, указываются диагностические возможности нейросонографии и магнитно-резонансной томографии в ее определении. Описаны клинические проявления и исходы данной патологии.
The article has data on the mechanisms of occurrence, risk factors, the frequency of occurrence of periventricular leukomalacia (PVL) in premature infants. The classification features of various types of PVL are given, the diagnostic capabilities of neurosonography and magnetic resonance imaging in its definition are indicated. There is a description of the clinical manifestations and outcomes of this pathology.
Pulpitis of Primary Teeth. Therapy
Authors: Рубникович С.П. , Андреева В.А. , Бинцаровская Г.В. , Валеева З.Р. , Кузьменко Е.В. 
Published: March 2022

Сохранение всех временных зубов до прорезывания постоянных зубов является одной из важнейших задач детской стоматологии. Тактика и выбор метода лечения пульпитов у детей зависит от ряда факторов: возраста и общего состояния ребенка, контактности его и родителей, кариесрезистентности, степени активности течения кариозного процесса, формы пульпита, стадии формирования корней.Сравнительный анализ результатов витальной пульпотомии и девитальной ампутации выявил значительную эффективность витальной пульпотерапии – 79,8–84,2% по сравнению с девитальной – 38,8–45,3%.В данном сообщении представлены современные витальные методы лечения пульпитов в зависимости от клиники, возраста ребенка и степени формирования корней, а также используемых материалов.
Saving of all primary teeth before eruption of permanent teeth is one of the most important tasks in pediatric dentistry. Treatment tactics and choice of pulp therapy method in children depend on several factors: age and general health of the child, patient’s cooperation, caries resistance, level of caries activity, form of pulpitis, stage of root formation.A comparative analysis of the results of vital pulpotomy and devital amputation revealed a significant effectiveness of vital pulp therapy – 79.8–84.2% compared to devital – 38.8–45.3%.This report introduces contemporary vital methods of pulp treatment depending on the clinic picture, child’s age, and root formation stage, along with the materials used.
Synthesis of SrSiO3:Tb3+, Eu3+, Li+ red phosphors for WLED and study of their luminescence properties
Authors: Wang Lei , Jilili Subiyinuer , Tuerxun Ayinuremu , Sidike Aierken , Wang Qingling 
Published: March 2022

Abstract
A series of Tb3+, Eu3+, Li+ co-doped SrSiO3 phosphors were prepared using the high-temperature solid-phase method. The X-ray diffraction results showed that Tb3+ and Eu3+ successfully entered into the SrSiO3 matrix lattice. Strong energy transfer from Tb3+ to Eu3+ is exhibited with a calculated energy transfer efficiency of 94.7%. Doping of the charge compensator Li+ increased the luminescence intensity of the SrSiO3:6%Tb3+, 6%Eu3+, 3%Li+ phosphor at a wavelength of 614 nm by 4.2 times and significantly improved the color purity of this phosphor (C
p = 85.9%). Based on color coordinate analysis, the phosphor color coordinates are located near (0.615, 0.365), which belongs to the red region. The phosphor was integrated into a white LED. The resultant package exhibited a color temperature of 6479 K and a color rendering index of 93.8. Its luminous performance was also very stable under different drive currents.
PENERAPAN METODE BASED TRACKING SEBAGAI MEDIA INFORMASI PADA MUSEUM SENI RUPA DAN KERAMIK JAKARTA
Authors: Nasir Abdul , Budiyanto Utomo 
Published: March 2022

Saat ini banyak sekali tempat hiburan yang menerapkan berbagai teknologi untuk menarik minat
masyarakat. Sehingga membuat tempat museum kurang diminati oleh masyarakat, karena kurang menariknya penyajian dari museum tersebut dalam mengenalkan benda sejarah yang ada didalamnya. Sehingga membuat masyarakat enggan mengunjunginya dikarenakan terlalu membosankan bagi mereka. Teknologi saat ini diharapkan mampu membuat proses pembelajaran mengenal benda museum dengan lebih canggih dan menarik. maka dari itu pihak Museum Seni Rupa dan Keramik sangat mendukung aplikasi augmented Reality (AR). Tujuan penelitian ini untuk menggunakan aplikasi augmented reality (AR) diharapkan pengelola museum Seni Rupa dan Keramik dapat menarik minat masyarakat dalam mengunjungi museum untuk mengenal benda sejarah dengan cara yang berbeda. Aplikasi ini diharapkan dapat membantu pengelola dalam proses pengenalan benda seni dan sejarah, sehingga mengunjungi museum lagi tidak menjadi hal yang membosankan lagi dan sehingga membuat masyarakat lebih mengenal karya seni dan benda sejarah yang ada. Dalam penelitian ini menggunakan metode Marker Based Tracking dengan bantuan aplikasi Unity 3D dan Vuforia untuk membuat aplikasi augmented reality (AR). Hasil Use Acceptance Test dari penelitian ini mendapatkan hasil yang baik dari masyarakat pengunjung museum dengan skor 393 dari 500
Schwarzschild and Ledoux are Equivalent on Evolutionary Timescales
Authors: Anders Evan H. , Jermyn Adam S. , Lecoanet Daniel , Fraser Adrian E. , Cresswell Imogen G. , Joyce Meridith , Fuentes J. R. 
Published: March 2022

Abstract
Stellar evolution models calculate convective boundaries using either the Schwarzschild or Ledoux criterion, but confusion remains regarding which criterion to use. Here we present a 3D hydrodynamical simulation of a convection zone and adjacent radiative zone, including both thermal and compositional buoyancy forces. As expected, regions that are unstable according to the Ledoux criterion are convective. Initially, the radiative zone adjacent to the convection zone is Schwarzschild unstable but Ledoux stable due to a composition gradient. Over many convective overturn timescales, the convection zone grows via entrainment. The convection zone saturates at the size originally predicted by the Schwarzschild criterion, although in this final state the Schwarzschild and Ledoux criteria agree. Therefore, the Schwarzschild criterion should be used to determine the size of stellar convection zones, except possibly during short-lived evolutionary stages in which entrainment persists.