bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single Cell Analysis Identifies Distinct Profiles in Pediatric Acute Respiratory Distress Syndrome
Authors: Flerlage Tim , Crawford Jeremy , Allen Emma , Severns Danielle , Tan Shaoyuan , Surman Sherri , Ridout Granger , Novak Tanya , Randolph Adrienne , West Alina , Thomas Paul 
Published: September 2022

Abstract
Acute respiratory distress syndrome (ARDS), termed pediatric ARDS (pARDS) in children, is a severe form of acute respiratory failure (ARF) that is associated with significant morbidity and mortality. Pathologic immune responses have been implicated in the development of pARDS. Here, we present a unique description of microbial sequencing and single cell gene expression in tracheal aspirates (TAs) obtained longitudinally from infants admitted to the pediatric intensive care unit (PICU) with ARF. Important alterations in TA epithelial cell, mononuclear phagocyte (MNP), and neutrophil transcription were associated with subjects’ illness severity, etiology (of ARF), and sampling time point. Specifically, in patients with moderate to severe pARDS compared to those with no to mild pARDS we identified reduced interferon stimulated gene (ISG) and cytokine expression in epithelial cells, reduced development over time of a regulatory IL-10 expressing macrophage population, and progressive airway neutrophilia associated with a unique transcriptional profile in aged neutrophils. In addition, we incorporated viral capture sequencing (ViroCap) with single cell transcriptomic analysis to explore interactions between respiratory syncytial virus (RSV) and host cells in infected and bystander epithelia. Our findings indicate that pARDS is defined by distinct inflammatory cell profiles that are etiology- and severity-dependent and implicate inadequate induction of ISGs, cytokines/chemokines, and repair-associated macrophage transcriptional programs in the pathogenesis of moderate to severe pARDS.
Effects of attentional focus instructions and autonomy support on learning a shooting task
Authors: Sadowski Jerzy , Chaliburda Agata , Wołosz Paweł , Mastalerz Andrzej 
Published: September 2022

Abstract
Research related to motor skill learning has provided an important insight into which instructional methods are effective. Recent research has shown that the combination of autonomy and external focus of attention may benefit learning outcomes; however, there is not enough strong evidence in this respect. The study sought to examine effects of attentional focus instructions and combined effects of autonomy (choice) and external focus of attention on the learning of a standing korfball shot. One hundred and seven postgraduate students of physical education (40 females and 67 males, age = 22.57) took part in the study. They were randomly assigned to one of four practice groups: external focus (E), internal focus (I), self-regulated (S) and self-regulated external (SE). During the acquisition phase, all participants completed a total of 150 trials, with 50 trials (5 sets x 10 repetitions) completed during each session. The results of the between-group comparison for shooting accuracy demonstrated no significant differences. The present findings suggest that external focus of attention benefits motor learning and performance. We did not reveal additive advantages for motor learning when comparing combined effects of external focus and autonomy support with each of these conditions administered separately.
Radiomics Based on Lumbar Spine CT Imaging to Detect Osteoporosis
Authors: yang qichao , Yang Hui , Shi Qiang , Pian Ying , Li Shanshan , Song Mingxin , Qin Jian 
Published: September 2022

Abstract
Purpose:This study aims to explore a radiomics model based on lumbar spine CT imaging for detecting osteoporosis.
Method:A retrospective analysis of 170 patients who underwent both CT and dual x-ray absorptiometry (DXA) of the lumbar spine in our hospital.According to the DXA results of these patients, 50 patients were normal, 50 patients had osteopenia, and 70 patients had osteoporosis.A total of 851 radiomic features were extracted from the images of the segmentation images in the lumbar CT imaging and the most significant radiomic features were selected to establish fourclassification models: normal vs osteoporosis , normal vs osteopenia, osteopenia vs osteoporosis，normal vs abnormal patients.The estimated area under the receiver operating characteristic curve（ROC）was used to assess the effectiveness of the classification models.
Results :The area under the curve (AUC) was 0.942 (95% CI, 0.919-0.964) and 0.933 (95% CI, 0.896-0.971) for the model of normal vs.osteoporosis in the training and validation cohorts, respectively; 0.738 (95% CI, 0. 683-0.792) and 0.749 (95% CI, 0.663- 0.835) for the model of normal vs. Osteopenia ;0.822 (95% CI, 0.778-0.865) and 0.777 (95% CI, 0.700-0.853) for the model of osteopenia vs. Osteoporosis；0.867(95% CI, 0.833-0.900) and 0.825 (95% CI, 0.768-0.882) for the model of normal vs. abnormal.
Conclusion: Radiomics based on lumbar CT imaging has great application value in diagnosing abnormal bone mineral density（BMD）.
A hydroxypropiophenone based fluorescent probe for selective determination of Al3+ ions in aqueous ethanol
Authors: Singh Divya Pratap , Singh Chandni , Singh Sunil Kumar , Dwivedi Romi , Singh Ashish Kumar , Singh Vinod Prasad 
Published: September 2022

Abstract

In this work, we had synthesized a novel dihydrazone-based fluorescent probe N'1,N'2-bis{1-(2-hydroxyphenyl)propylidene}oxalohydrazide for Al3+ by simple condensation reaction. The prepared organic probe has been characterized by different physicochemical and spectroscopic techniques. The single-crystal structure of the receptor has also been reported. Aqueous ethanolic solution of probe shows an enhanced fluorescence in the presence of Al3+ ions whereas no appreciable change in spectral pattern is observed in presence of other cations viz. Na+, K+, Ca2+, Ba2+, Mn2+, Fe3+, Co2+, Ni2+, Cu2+, Zn2+, Cr3+, Cd2+ and Hg2+. The binding mode of the receptor with Al3+ was studied by various spectral titration techniques such as UV-visible, fluorescence and 1H NMR. The receptor acts as a dibasic hexadentate ligand and interacts with two Al3+ ions with a high binding constant KB = 8.99 × 1010 M− 1. The lowest detection limit for the Al3+ complex of H2hpoh was determined to be 7.8 × 10− 5 M. With the help of DFT calculation, the mechanism of fluorescence enhancement has been explained.
Effect of hemicellulose extraction on the solution properties of cellulose carbamate in NaOH/ZnO aqueous system
Authors: Shang Jiaoping , Wang Junmei , Li Yibao , Peng Yun , Li Xingxing , Zhou Jinping , Xu Dingfeng 
Published: September 2022

Abstract
Hemicellulose removal from bleached bamboo pulp is the key to produce of qualified dissolving pulps. In this work, the alkali/urea aqueous solution was applied to improve the hemicellulose removal efficiency of bleached bamboo pulp. The removal rate of hemicellulose was affected by urea usage, time and temperature. The removal rate of hemicellulose achieved 70.14% in 6 wt% NaOH/1 wt% urea at 40°C for 30 min. After esterification by urea method of the pulp, the dissolution behavior of cellulose carbamate (CC) pulp with different DP, hemicellulose and N contents were studied, and the highest solubility was up to 97.7%. The effect of hemicellulose content on the stability and solubility of CC solution was subsequently analyzed by polarizing microscope and rheology. With a decrease of hemicellulose from 15.9, 8.60 to 5.70%, the gel temperature increased from 59.0, 69.0 to 73.4°C. The apparent gelation time increases from 5640 to 12120 s with the hemicellulose decreased from 15.9 to 8.60%, and HC-5.70 always keeps a liquid-state (G'' > G') until the test time reach 17000 s. The results showed that the removal hemicellulose, reduce DP and increasing esterification endow CC solution with higher solubility and stability.
An Evaluation of Vascular Density Changes by Optical Coherence Tomography Angiography in Patients with Ocular Hypertension
Authors: Çomçalı Sebile , Yılmaz Pınar Topçu , Köz Özlem Gürbüz , Coşkun Çiğdem , Çavdarlı Cemal , Yarangümeli Alper , Özdemir Emine Yıldız 
Published: September 2022

Abstract
Purpose
To evaluate Optical Coherence Tomography Angiography (OCT-A) findings in patients with Ocular Hypertension (OHT) and compare them with healthy individuals.
Material and Methods
Thirty-four patients with ocular hypertension (OHT) and 22 healthy individuals were included in the study. Foveal thickness, retinal vascular density in superficial and deep capillary plexus and choriocapillaris, foveal avascular zone (FAZ), acircularity index (AI), foveal vessel density (FD), non-flow area, capillary and all vessel densities in the peripapillary area and the disc were automatically measured using the Angiovue software of OCT-A and compared between groups.
Results
The comparison of the macular OCT-A findings did not reveal a significant difference between the two groups in terms of central macular thickness, superficial and deep capillary plexus vessel density (p > 0.05). The foveal avascular zone width was significantly higher in OHT patients compared to the control group (0.30 ± 0.08µ and 0.25 ± 0.11 µ, respectively; p = 0.04). The comparison of optic nerve OCT-A findings revealed that the whole-field vessel density (wVD) (p = 0.007), peripapillary vessel density (pVD) (p = 0.001), inferior, superior and temporal radial peripapillary capillary plexus vessel density (p = 0.006, p = 0.008, p = 0.02) and the mean retinal nerve fiber layer thickness (p = 0.02) were significantly lower in the OHT group.
Conclusion
Our findings suggest that the decrement in the optic disc vascular density and foveal avascular zone width was significantly higher in OHT patients. The possible effect or role of these microvascular changes in terms of glaucoma development should be examined through further studies.
Effects of Jacalin- a galactose binding lectin on MDA-MB-468, a triple-negative breast cancer cell line, and its combinatorial effect with taxol
Authors: Kumar Bommanaboina Anil , Waseem Mohammad , Jamal Shazia , Ahmed Neesar 
Published: September 2022

Abstract
Plant lectins have been well established to show the affect on the proliferation in different human cancer cells. The anti-proliferative potential of lectins have been ubiquitously investigated from diverse sources and, the basic mechanism has been explored in an elaborated index. In our study, we observed anti-proliferative efficacy of Jacalin (a purified lectin from jackfruit (Artocarpus integrifolia) seeds in MDA-MB-468, a triple-negative breast cancer cell line. This effect was explored in a dose dependent manner. The cells were treated with Jacalin at different time points (6hr and 12hr) and thereafter the recovery in cell propagation was also observed after which the jacalin media was aspirated. Thus, Jacalin was considered as to possess reversible effects on MDA-MB-468 cells. On the other side, Jacalin did not pose the effect in proliferation cascade in PBMCs, taken as primary cell line control. Galactose is well recognized ligands of Jacalin and its pre-incubation could neutralize the effect. Furthermore, MDA-MB-468 cells were also treated with Jacalin in combination with increasing concentration of taxol, keeping jacalin concentration constant (40µg/ml). Taxol at the concentration of 930 nM in combination with jacalin achieved the similar kind of anti-proliferative effect that was observed with 30 µM of taxol. Thus the taxol concentration could be reduced if given in combination of jacalin, preventing the side effect of taxol due to higher dose.
Whole-Brain Structure-Function Coupling Abnormalities in Mild Cognitive Impairment: A Study Combining ALFF and VBM
Authors: Zhao Rong , Wang Pan , Zhang Fanyu , Hu Peng , Wen Jiaping , Li Hongyi , Biswal Bharat B. 
Published: September 2022

Abstract
Background
Alzheimer's disease, one of the most leading nervous system diseases, is accompanied by symptoms including loss of memory, thinking, and language ability. Both mild cognitive impairment (MCI) and very MCI (VMCI) are the transitional pathological stage between normal ageing and AD. While the changes to whole-brain structural and functional information have been extensively investigated in AD, the impaired structure-function coupling within whole brain remains unknown.
Methods
Current study employed the OASIS-3 dataset including 53 MCI, 90 VMCI and 100 age-, gender- and education-matched normal controls (NC). Several structural and functional parameters including amplitude of low frequency fluctuations (ALFF), voxel-based morphometry and ALFF/VBM ratio analysis were used to estimate the whole-brain abnormalities among MCI, VMCI and NC.
Results
As the disease symptoms became more severe, these regions distributing in the cerebellum and putamen within gray matter exhibited progressively increasing ALFF (ALFFNC < ALFFVMCI < ALFFMCI). Similar results were also found in the frontal-inf-orb, putamen, and paracentral-lobule within white matter. More importantly, as the symptoms of disease got worse, parahippocampal gyrus and hippocampus within gray matter showed progressively decreasing structure-function coupling, and was also applicable to the cuneus and frontal lobe within WM. In addition, the structure-function coupling values in the parahippocampal gyrus and hippocampus were positive relationship with severity of cognitive impairment, suggesting the important applications of the structure-function coupling index in brain disorders.
Conclusion
Our findings provided a novel information for discovering the pathophysiological mechanisms and indicated that WM lesions were also an important cause of cognitive decline in AD.
Geochemical precursory characteristics of soil gas related to the 2019 Xiahe Ms5.7 earthquake across the northern margin of West Qinling fault zone,Central China
Authors: Zhou Huiling , Li Zongxing , Su Hejun , Li Chenhua , Wan Yue 
Published: September 2022

Abstract
The Xiahe Ms5.7 earthquake occurred on October 28, 2019, and was located within the national earthquake priority hazard zone designated as of 2019. We use the mobile observation data of soil gases Rn, Hg, H2, and CO2 from 2016 to 2019 of 11 profiles in the northern margin of the West Qinling fault zone, combining the sturdy body seismogenic model and numerical simulation results to investigate the spatial and temporal evolution characteristics of the cross-fault soil gas before the Xiahe Ms5.7 earthquake. This research showed that the seismic activity of the northern margin of the West Qinling fault zone varies significantly among the secondary fault zones, and the overall state is that the middle east section’s activity is weakened toward the west section, which has good coupling with the spatial distribution characteristics of the fault soil gas in this fault zone. The soil gas concentration intensity across the northern margin of the West Qinling fault zone showed a clear decreasing trend from HT1 to HT4 in the western section, which is the closest segment to the Xiahe Ms5.7 earthquake fault, and a turning up after a continuous decrease in Rn in HT1 and Hg and CO2 in HT2, and Rn, CO2, and Hg in HT4. The other measurement lines in the middle east section did not show similar precursor features. The decline characteristic of fault gas tectonic geochemistry is a good indicator of the interlocked section of fracture tectonic activity. The sturdy body seismogenic model can explain the relationship between the tectonic geochemical characteristics and the rupture locking, and it has theoretical support for determining the time, space, and intensity of seismogenesis. Strengthening the research on seismic models, obtaining a physical model that is “close to reality,” and generating a comprehensive and unified interpretation of the precursor field under the guidance of certain models is a practical approach for earthquake prediction and forecasting in the future. It is easy to make mistakes in terms of the location if we do not identify the mechanism. Thus, it is crucial to strengthen the flow monitoring and seismogenic model research of the deep fluids in the fracture zone.
Wildfire exerts a long-term impact on soil multifunctionality by influencing soil environments and vegetation regeneration in a Chinese boreal forest
Authors: Kong Jianjian , Yu Yue , Xiang Xingjia , Yang Jian 
Published: September 2022

Abstract
Background and aims
Wildfires can trigger significant changes in soil functioning in boreal forests, and fire-caused changes in soil environments and vegetation community can further affect soil functioning. However, the response of multiple simultaneous soil functions (i.e. multifunctionality) to wildfire and postfire changes in soil environments and regeneration vegetation remains unknown though soil multifunctionality (SMF) is crucial to the biogeochemical cycling and plant growth.
Methods
We sampled 60 soils at unburned control, 1-year-postfire, and 11-year-postfire sites in a Chinese boreal larch forest to explore the relationships between SMF and fire severity, bacterial diversity, soil environments and regenerated vegetation.
Results
The SMF was lower at the two burned sites relative to the control, decreasing with increased fire severity but increasing with soil bacterial diversity. Structural equation modeling analysis confirmed that fire severity was the most important predictor of SMF at the 1-year-postfire site, while SMF at the 11-year-postfire site was mainly mediated by soil moisture besides fire severity, regenerated tree density and grass cover.
Conclusions
Wildfire exerts a long-term negative effect on SMF and this effect is closely related to fire severity and bacterial diversity in the Great Xing’an Mountains. The effects of wildfire on SMF might be directly mediated by fire severity in the short term but indirectly by altering soil environments and vegetation regeneration in the long term. We conclude that increasing fire severity of these forests can both directly decrease SMF and trigger the degradation of their soil-plant ecosystems, which would further imperil the maintenance of SMF.
E2F1 activation by oncogenic FLT3 internal tandem duplication in regulating purine metabolism in acute myeloid leukaemia
Authors: Chen Feihu , Ou Zi-yao , Wang Ke , Shen Wenwen , Deng Ge , Xu Yayun , Wang Longfei , Zai Zhuoyan , Ling Yian , Zhang Tao , Peng Xiaoqing 
Published: September 2022

Abstract
Oncogene FLT3 internal tandem duplication (FLT3-ITD) mutation account for up to 30% of acute myeloid leukaemia (AML) cases and induce transformation.Previously, we found that E2F transcription factor 1 (E2F1) is involved in autophagy in the AML cell line MOLM-13, which harboring the FLT3-ITD. Here, we reported that E2F1 expression was gradually decreased during normal haematopoiesis but was aberrantly upregulated in AML patients carrying FLT3-ITD. E2F1 knockdown in cultured FLT3-ITD-positive AML cells inhibited cell proliferation and increased their cellular sensitivity to all-trans retinoic acid (ATRA) and its derivative 4-amino-2-trifluoromethyl-phenyl resinate (ATPR). Moreover, E2F1-depleted FLT3-ITD+ AML cells lost their malignancy as shown by the reduced leukaemia burden and prolonged survival in leukemic NOD-PrkdcscidIl2rgem1/Smoc mice. Additionally, FLT3-ITD-driven transformation of human CD34+ haematopoietic stem and progenitor cells (HSPCs) was counteracted by E2F1 knockdown. Mechanically, the expression and nuclear accumulation of E2F1 were highly dependent on FLT3-ITD activity. Further study using chromatin immunoprecipitation-sequencing and metabolomics analyses revealed that ectopic FLT3-ITD promoted the recruitment of E2F1 on genes encoding key enzymatic regulators of purine metabolism and thus supported AML cell proliferation. Together, this study demonstrates the dependence of FLT3-ITD+ AML cells on E2F1-regulated purine metabolism, and provides a promising therapeutic strategy for AML patients.
Authoritarian Leadership and Nurse Presenteeism: The Role of Workload and Leader Identification
Authors: Shan Geyan , Wang Wei , Wang Shengnan , Zhang Yongjun , Guo Shujie , Li Yongxin 
Published: September 2022

AbstractBackground:Nurses’ health in the workplace is crucial for ensuring the quality of healthcare. However, presenteeism, the behavior of working in a state of ill health, is widespread in the nursing industry. Considering that the origin of authoritarian leadership and the prevalence of presenteeism are inseparable from Chinese workplace culture, this study aimed to explore the impact and mechanism of authoritarian leadership on presenteeism.Methods:A total of 528 nurses were recruited from four 3A-grade hospitals in the present survey, which was distributed across 98 nursing teams. Participants were required to complete self-report measures on authoritarian leadership, presenteeism, workload, and leader identification. Description, correlation, and multilevel linear regressions were applied for data analysis.Results:The present study found that presenteeism was significantly related to participants’ demographic characteristics, such as marital status, educational level, technological title, and general health. There was a positive relationship between authoritarian leadership and presenteeism, and workload acted as a mediator in authoritarian leadership and presenteeism. Furthermore, leader identification moderated the relationship between authoritarian leadership and workload. When nurses were under high leader identification, the positive impact of authoritarian leadership on workload was reinforced.Conclusions:This study revealed the potential antecedents and mechanisms of nurse presenteeism from the perspective of workplace culture. Results indicated that the excessive authoritarianism of leaders and the heavy workload faced by nurses may be the significant triggers for nurses' presenteeism. The role of leader identification is not always protective, which may heighten the relationship between dark leadership and its outcomes. These observations contribute to enriching research on presenteeism and authoritarian leadership, and provide valuable insights for cultivating healthy working behaviors.