bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence factors, clinical manifestations and prognosis of left atrial thrombosis in atrial fibrillation
Authors: Yang Qian , Liu Shuang , Wang Jiang , Guo Yulong 
Published: August 2022

Abstract
Background: We did this study to find out the influence factors, clinical manifestation and prognosis of left atrial thrombosis and its relationship with systemic thromboembolism, hoping to find a better therapy strategy. Methods: A single-centre retrospective study enrolled patients with definite diagnosis of atrial fibrillation complicated with left atrial thrombosis. Data of general clinical information, anticoagulation medications, thromboembolism events and thrombosis prognosis were recorded and analyzed. Results: 103 patients were enrolled. Compared with non-valvular atrial fibrillation (NVAF), thrombosis outside the left atrial appendage (LAA) was much more common in valvular atrial fibrillation (VAF) with p=0.003. Total prevalence of systemic thromboembolism was 33.0%. Thrombi disappeared in 78 cases (75.7%) under anticoagulation treatment within two years. There was no significant difference between warfarin, dabigatran and rivaroxaban on thromboembolism events and thrombosis prognosis in NVAF, with p=0.740 and 0.493, respectively.Conclusions: Atrial fibrillation patients with severe LA remodeling have really high risks of LA thrombosis and systemic thromboembolism. Compared with NVAF, Patients with VAF are more likely to develop the whole LA dysfunction rather than the only LAA dysfunction, manifested as more thrombosis outside the LAA were found. Standard stroke preventing dose of anticoagulants couldn’t deplete all LA thrombi.
Cancer/testis antigen CAGE mediates Osimertinib Resistance in Non-small cell lung cancer cells and predicts poor Prognosis in Patients with Pulmonary adenocarcinoma
Authors: Yeon Minjeong , Lee Hankyu , Yeo Jeongseon , Jeong Myeong Seon , Jung Hyun Suk , Lee Hyerim , Shim Kyeonghee , Jo Hyein , Jeon Doyong , Koh Jaemoon , Jeoung Dooil 
Published: August 2022

Abstract
Purpose
CAGE, a cancer/testis antigen, was originally isolated from the sera of patients with gastric cancers. We have shown the role of CAGE in resistance to chemotherapy and target therapy. In this study, we wanted to investigate the possible role of CAGE in osimertinib, an inhibitor of EGFR tyrosine kinase.
Methods
The clinicopathological correlation with CAGE and autophagy flux in patients was examined using immunohistochemistry and in situ hybridization. The evaluation of autophagy in osimertinib resistance was analyzed using immune-blot, Immuno-cell chemistry and immuno-histochemistry in vitro and in vivo.
Results
Here, we found that IHC showed the expression of CAGE in more than 50% of patients with pulmonary adenocarcinomas (pADCs). The expression of CAGE was increased in pADCs after the acquisition of EGFR-TKIs resistance. High expression of CAGE was correlated with shorter overall survival (OS) and progression free survival (PFS) in patients with pADCs. Thus, CAGE mediates osimertinib resistance and predicts poor prognosis in patients with pADCs. Osimertinib-resistant non-small cell lung cancer cells (PC-9/OSI) were established. Mechanistic studies of CAGE-mediated osimertinib resistance were performed. PC-9/OSI cells showed increased autophagic flux and CAGE expression compared with parental sensitive PC-9 cells. PC-9/OSI cells showed higher tumorigenic, metastatic, and angiogenic potential compared with parental PC-9 cells. CAGE CRISPR-Cas9 cell lines showed decreased autophagic flux, invasion, migration potential, and tumorigenic potential compared with PC-9/OSI cells in vitro and in vivo.
Conclusion
Collectively, our data suggest that CAGE plays a crucial role in the progression of tumorigenesis and metastasis by modulating autophagy. Furthermore, our findings propose the inhibition of CAGE as a potential therapeutic strategy for osimertinib resistance.
ABAQUS and ANSYS implementations of peridynamics-based finite element method (PeriFEM) for brittle fracture
Authors: Han Fei , Li Zhibin , Zhang Jianyu , Liu Zhiying , Yao Chen , Han Wenping 
Published: August 2022

Abstract
PeriFEM is a reformulation of traditional FEM for solving peridynamic equations numerically. It takes the nonlocal feature of peridynamics into account, and thus possesses the same computational framework as traditional FEM. Such advantages enable the convenient integration of peridynamics and commercial FEM software. Therefore, in the current work, we address the basics of PeriFEM within the framework of traditional FEM first, based on which, we propose a unified implementation technique for peridynamics in the commercial FEM software. And we implement the peridynamic simulation in ANSYS and ABAQUS, respectively. The implicit algorithm is used in both software, but different convergence criteria are adopted due to their own features. In ANSYS, APDL allows users to get the broken-bond information from UPFs conveniently, thus whether there are new broken bonds is adopted as the convergence criterion. In ABAQUS, it is not convenient for users to obtain the broken-bond information, thus the default convergence criterion in ABAQUS is used. The code that is integrated into ANSYS and ABAQUS are both verified through benchmark examples. The computational convergence and computational cost are compared. The results show that ABAQUS is more efficient, whereas the convergence criterion adopted in ANSYS is more robust. Finally, 3D examples are carried out to prove the ability of the proposed approach in dealing with complex engineering problems.
N6-methyladenosine-modified VGLL1 promotes ovarian cancer metastasis through HMGA1/Wnt/β-catenin signaling
Authors: Xia Jian-Chuan , Li Han , Cai Liming , Pan Qiuzhong , Jiang Xingyu , Zhao Jingjing , Xiang Tong , Tang Yan , Wang Qijing , He Jia , Weng Desheng , Zhang Yanna , Liu Zhongqiu 
Published: August 2022

Abstract
The main causes of death in ovarian cancer (OC) patients are invasive lesion and the spread of metastasis. Determining the biology of cancer metastasis is important to develop novel targeted therapy. The present study aimed to explore the mechanisms that might promote OC metastasis. Here, we identified that VGLL1 expression was remarkably increased in metastatic OC samples. The role of VGLL1 in OC metastasis and tumor growth were examined by cell function assays and mouse models. Mechanistically level, METTL3-mediated N6-methyladenosine (m6A) modification contributed to VGLL1 upregulation in an IGF2BP2 recognition-dependent manner. Furthermore, VGLL1 directly interacts with TEAD4 and co-transcriptionally activates HMGA1. HMGA1 further activates Wnt/β-catenin signaling to enhance OC metastasis by promoting the epithelial-mesenchyme transition traits. Rescue assays indicated that upregulation of HMGA1 was essential for VGLL1-induced metastasis. Additionally, conditional silencing of VGLL1 using a doxycycline-induced knockdown system showed therapeutic potential against metastasis in OC. Importantly, the clinical relevance of IGF2BP2, VGLL1, HMGA1, and β-catenin was conformed in OC tissues. Collectively, these findings showed that m6A-induced VGLL1/ HMGA1/β-catenin axis might play a vital role in OC metastasis and tumor growth. VGLL1 might serve as a prognostic marker and therapeutic target against metastasis of OC.
The state of nursing and midwifery leadership, governance structures, and instruments in Africa
Authors: Nawagi Faith , Kneafsey Rosie , Modber Mohammed , Mukeshimana Madeline , Ndungu Cecilia , Bayliss-Pratt Lisa 
Published: August 2022

Abstract
Background Investment in nursing and midwifery leadership and governance are key suggested approaches by the World Health Organization (WHO) Africa Regional Office to address the shortages of the nursing health workforce. However, there are few if any studies that have investigated the existence and operationalization of the nursing leadership and governance structures in Africa. This paper fills this gap by, providing an overview of nursing and midwifery leadership, governance structures, and instruments in Africa.MethodsWe conducted a descriptive cross-sectional study of the nursing and midwifery leadership ,structures and instruments in 16 African countries using quantitative methodology. Data was analyzed using SPSS IBM 21 statistical software. Data was summarized in frequencies and percentages and presented as tables and charts. ResultsOnly(9,56.25%) of the 16 countries included had retrievable evidence of all expected governance structures while (7, 43.75%) lacked one or more of the structures. A quarter (4, 25%) of the countries did not have a department of nursing and midwifery or chief nursing and midwifery officer at their Ministry of Health (MOH). The dominant gender representation across all the governance structures was female. Only Lesotho (1, 6.25%) had all expected nursing and midwifery governance instruments while the remaining (15, 93.75%) had either one or four of these instruments missing.ConclusionsThe lack of complete nursing and midwifery governance structures and instruments in various African countries is a matter of concern. Without these structures and instruments, the strategic direction and input of the nursing and midwifery profession cannot be maximized for the public good in relation to health outcomes. Addressing the existing gaps requires a multipronged approach with the need to strengthen regional collaboration, advocacy, creating awareness, and advancing nursing and midwifery leadership training to enable nursing and midwifery governance capacity development in Africa.
Dielectric strength of nanofluid-impregnated transformer solid insulation
Authors: Pérez-Rosa Daniel , Montero Andrés , García Belén , Burgos Juan Carlos 
Published: August 2022

Abstract
The interest in developing new fluids that can be used as dielectric liquids for transformers has driven the research on dielectric nanofluids in the last years. A number of authors have reported promising results on the electrical and thermal properties of dielectric nanofluids. Less attention has been paid to the interaction of these fluids with the cellulose materials that constitute the solid insulation of the transformers. In the present study, the dielectric strength of cellulose insulation is investigated, comparing its behaviour when it is impregnated with transformer mineral oil and when it is impregnated with a dielectric nanofluid. The study includes the analysis of the AC breakdown voltage and the impulse breakdown voltage of the samples. Large improvements were observed on the AC breakdown voltages of the specimens impregnated with nanofluids while the enhancements were lower in the case of the impulse tests. The reasons for the increase in AC breakdown voltage were investigated considering the dielectric properties of the nanofluids used to impregnate the samples of cellulose. The analysis was completed with a finite element study that revealed the effect of the nanoparticles on the electric field distribution within the test cell, and its role in the observed enhancement.
Transcriptomic study of the cell death-related pathways of cervical cancer HeLa cells in response to Clinacanthus nutans treatment
Authors: Adum Kristine Sandra Pey , Haron Nor Hasyimah binti , Toha Zaleha binti , Arsad Hasni bin 
Published: August 2022

Abstract

Background
Recent years have witnessed major development of novel therapeutic agents like chemotherapy, targeted therapy and immune checkpoint inhibitors for cervical cancer. However, cervical cancer remains prevalent, leading to a large number of deaths worldwide. A better understanding of the cervical cancer biology and signaling pathways might lead to the development of targeted therapies in reducing the incidence and mortality rate.
Methods
In this study, the RNA-Seq reads of HeLa cells treated with C. nutans were compared to the untreated sample. The reads of these two sample groups were firstly aligned to the human reference genome. The results in BAM files format that were generated were then sorted before being assembled. The output of assembly which was in coverage table form was ready for downstream statistical analyses for differential expression. Differentially expressed genes were obtained and the cell-death related pathway were identified by canonical pathway, QIAGEN Ingenuity Pathway Analysis (IPA). The verification of significant genes was carried out using qRT-PCR by including GAPDH as a housekeeping gene
Results
With this, we identified a total of 668 upregulated and 479 downregulated analysis-ready genes across observations upon cut-off setting log2FoldChange at 0.5 and P-value 0.05. A total of 28 cell-death related canonical pathways and 4 activation of cell-death related functions were identified. Upon analyses, we identified four significant genes (Casp9, KAI1, REL and FOXO4) that hold important role in promoting cell death. These findings were also verified against the quantification using qRT-PCR by including GAPDH as a housekeeping gene.
Conclusions
This study provides an insight on the potential role of DCM fraction of C. nutans in activating Casp9, KAI1, REL and FOXO4 genes in mediating apoptosis in cervical cancer cells.
Current Status and System Construction of Used- Furniture Recycling in China
Authors: Xiong xianqing , Yue Xinyi , Dong Weihang , Xu Zhenzhong 
Published: August 2022

Abstract
In order to promote the development of the green and low-carbon wood industry and explore the current status and trend of China’s used-furniture recycling, this paper summarizes the research status of used-furniture recycling and interprets governmental strategies, such as the development of used-furniture recycling system, the way of handling used-furniture by manufacturers, and the attitudes of customers towards used-furniture, through extensive literature reviews and market researches. From such three respects as government, furniture manufacturers, and consumers, the problems of national used-furniture recycling are analyzed, for example, imperfect system, high cost and weak awareness. Therefore, to solve the above problems, it is necessary to construct national used-furniture recycling system and methods, including, for government, the construction of recycling network, the supervise and support of entrepreneurial responsibilities, and the formulation of recycling standards; for furniture manufacturers, the improvement of the durability and recyclability of furniture, the development of new models and new technologies for furniture recycling and transportation; and for consumers, the improvement of their concepts of consuming and the environmental protection. In sum, it aims to provide both suggestions for the amelioration of the measures and methods, and support for the understanding of the change, of used-furniture recycling in China.
Association between Metabolite Cotinine and Volatile Organic Compounds (VOCs) Serum Levels in Adults Living with HIV, HBV, or HCV: NHANES 2005–2018
Authors: Yang Jie , Zhang Hao , Lin Jin-Long , Liu Jing , Jiang Xiao-Wen , Peng Lei 
Published: August 2022

Abstract
Objectives: To identify the unilateral effect of VOCs on cotinine metabolism. Methods: Measurements of 13,652 participants included sociodemographic characteristics and serological indicators (VOCs and cotinine). Correlation between VOCs and cotinine and the effect of VOCs on cotinine metabolism were analyzed by Spearman analysis and multivariable logistic regression analysis. Results: Among smokers infected with HIV | HBV | HCV with the largest exposure dose to tobacco, the intensity of the association between VOCs and cotinine was the strongest. High concentrations of 1,2-Dichlorobenzene (OR: 1.101, CI: 1.069–1.134), Benzene (OR: 1.354, CI: 1.123–1.632), 2,5-Dimethylfuran (OR: 1.054, CI: 1.027–1.082) and Nitrobenzene (OR: 1.347, CI: 1.039–1.746) and low concentration of Carbon Tetrachloride (OR: 0.760, CI: 0.661–0.873) in blood might be independent risk factors leading to the increase of serum metabolite cotinine. Conclusions: The influence intensity of four VOCs (including 1,2-dichlorobenzene, benzene, carbon tetrachloride, 2,5-dimethylfuran and nitrobenzene) on serum cotinine metabolism has a dose-response relationship, that is, the higher the tobacco exposure, the stronger the influence intensity. In addition, this study used molecular epidemiology to indirectly provide new ideas and views on the harm of smoking for smokers.
Development and validation of a Fast Spine Protocol for Use in Paediatric Patients
Authors: Wu Winnie (Shu Yu) , Miller Elka , Hurteau-Miller Julie , Thipse Madhura , Kapoor Cassandra , McAuley David , Tu Albert , Webster Richard 
Published: August 2022

Abstract
ObjectiveConventional pediatric spine MRI protocols have multiple sequences resulting in long acquisition times. Sedation is consequently required. This study evaluates the diagnostic capability of a limited MRI spine protocol for selected common pediatric indications. MethodsAfter REB approval, records of pediatric patients under 4 years of age who underwent a spine MRI at CHEO between 2017 and 2020 were reviewed. Two neuroradiologists blindly and retrospectively reviewed the T2 sagittal sequences from the craniocervical junction to sacrum and T1 axial sequence of the lumbar spine, to answer specific questions regarding cerebellar ectopia, syrinx, level of conus, filum <2mm, fatty filum, and spinal dysraphism. The results were independently compared to previously reported findings from the complete imaging series. Results105 studies were evaluated in 54 male and 51 female patients (mean age of 19.2 months). The average combined scan time of the limited sequences was 15 minutes compared to 35 minutes for conventional protocols (delta = 20 minutes). The average percent agreement between full and limited sequences was >95% in all but identifying a filum <2mm, where the percent agreement was 87%. Using limited MR sequences had high sensitivity (>0.91) and specificity (>0.99) for the detection of cerebellar ectopia, syrinx, fatty filum, and spinal dysraphism. ConclusionThis study demonstrates that selected spinal imaging sequences allows for consistent and accurate diagnosis of specific clinical conditions. A limited spine protocol reduces acquisition time, potentially avoiding sedation. Further work is needed to determine the utility of selected imaging for other clinical indications.
Seroprevalence of Mycobacterium avium subsp. paratuberculosis (MAP) and associated risk factors in Northern Ireland dairy cattle herds
Authors: Guta Sintayehu , Allen Adrian , Graham Jordan. , Byrne Andrew W. , Milne Georgina , Angela Lahuerta-Marin , Drummond Lindsey , McInerney Barry , Campbell Emma , Conrad Watson , Strain Sam , Skuce Robin 
Published: August 2022

Abstract
BackgroundJohne’s disease is a chronic granulomatous enteritis of cattle and other ruminants of economic, animal and public health significance, caused by Mycobacterium avium subsp. paratuberculosis. It is endemic in UK, but there is currently limited information in Northern Ireland. To address this gap, for the first time surveillance data were used to estimate seroprevalence and explore risk factors for a cohort of herds that participate in a voluntary scheme. MethodsA Bayesian approach, Gibbs sampling was used to estimate animal-level true prevalence. The effects of test sensitivity, specificity, and true prevalence and number of animals tested and critical number of positive reactors were considered for herd test sensitivity, specificity and true prevalence estimation. Logistic regression was used to develop a model associating herd status with putative risk factors.Results46,715 cattle in 431 dairy herds were tested by ELISA between 2010 and 2019. 2138 (4.6%) animals were positive. At least one ELISA-positive case was detected in 239 herds (55.4%, 95%CI: 50.7 - 60.1) whereas, 152 herds recorded ≥2 ELISA-positives (35.2 %, 95% CI: 30.7 - 39.8). The mean true herd prevalence was estimated to be 34.7% and 43.9 % for k ≥1 and k ≥2 critical number of reactor herds respectively. The study estimated 9.5 % (95%PPI: 0.5 - 21.4), 32.8 % (95 % PPI: 17.1 - 50.8) and 98.3 % (95 % PPI: 95.6 - 99.9), median values for animal-level true prevalence, test sensitivity and specificity respectively.Herds sized ≥ 100 were between 1.8 and 5.5(95%CI: of the OR) times more likely to test MAP ELISA-positive than herds with < 100 cattle. Herds with mean animal age of ≥5 years were between 1.5 and 3.6 (95% CI: of the OR) times more likely to have MAP ELISA-positive results than herds with a mean animal age of < 5 years. ConclusionResults of this study estimated disease prevalence and identified some associated risk factors which improves understanding of the local epidemiology, and indicate the need for establishing a sustainable control programme. Further investigation is required to better understand local epidemiology and associated production and economic losses.
Eco-friendly synthesis of ZnO-nanoparticles using Phoenix dactylifera L. polyphenols: physicochemical, microstructural and functional assessment
Authors: ABDULLAH JOHAR AMIN AHMED , Jiménez-Rosado Mercedes , Guerrero Antonio , Romero Alberto 
Published: August 2022

Abstract
In the last few decades, nanoparticles (NPs) synthesis has evolved into a green nanotechnology field, requiring more eco-synthesis of nanoparticles due to the high costs of other chemical-physical methods. Among the most commonly used nanomaterials, ZnO-NPs are highly valuable due to their specific, thermal, optical, and electronic features. Thus, the main objective of this work was to investigate the green synthesis of ZnO-NPs employing Phoenix dactylifera L. extract, which is rich in polyphenols, as a reducing agent. In this way, the effect of the concentration of both the precursors and the reducing agent was evaluated. The NPs were compared through X-ray diffraction (XDR), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and Fourier infrared transformation spectroscopy (FTIR). Additionally, we evaluated the antioxidant properties (TAC and DPPH) and antibacterial activity of these nanoparticles against Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) pathogenic strains. The results show that it is possible to obtain ZnO-NPs using a green reducing agent (polyphenol extract), presenting a particle size between 18.1 and 61.6 nm. In addition, this synthesis highlighted the antioxidant and antibacterial activities of these nanoparticles. In conclusion, this method could be a suitable substitute for typical toxic methods for the synthesis of metallic nanoparticles.