bluuebunny/crossref_metadata_2025_split huggingface dataset:
Negotiating the language of gender and sexuality
Authors: Rowlett Benedict J. L. , Chhim Putsalun 
Published: October 2022

Abstract
This case study focuses on the educational materials created by an NGO drop-in centre for the queer/questioning community in a Cambodian city. These materials consist of bilingual posters (English and Khmer) on display at the centre which provide explanations to those who make use of this space about diverse gender/sexual identities (LGBTQ+), as well as online resources featured on the NGO’s website/social media that raise awareness of these issues at both local and global levels. The study seeks to gain critical insight into the use of certain linguistic resources for sexuality education at this site of instruction. To do so, we present a multimodal discourse analysis of a sample of the materials, together with an analysis of metapragmatic reflections drawn from interviews conducted with the centre’s director. We therefore attend to how multilingual linguistic resources, and other semiotic forms, are being used to foster and shape knowledges about gender and sexualities at this site of community engagement, and how a metapragmatic negotiation of these knowledges in the interview reveals identity work that impacts these linguistic choices and their potential effects.
An Application of Benchmarking Tool for Measuring the Performance of Urban Park
Authors: Mohd Hashim Nor Hanisah , Mohd Nasir Nurul Akmaniza , Isri Rabiatun Adawiyah 
Published: October 2022

Abstract
This study is conducted to evaluate the level of performance of recreational activities in selected urban parks. The objective of the study is to measure the comparative standard and performance of selected urban parks and the measure user’s perceptions and expectations of the quality of the products and services received by using Visitor Services Quality gap (VSQ gap) and Spider Web analysis. The case studies include two different urban parks in the vicinity of Klang Valley area, which are KLCC Park in Kuala Lumpur and Setia Alam Park in Setia Alam. Several criteria of standard, performance, the quality of the products and services that could contribute to the better performance of urban park have been identified.
Recycling cement bypass dust and granulated blast furnace slag into hydraulic road binder
Authors: Stevulova N , Junak J , Strigac J , Junakova N 
Published: October 2022

Abstract
This paper is aimed at the possibility of recycling the cement bypass dust (BPD) and ground granulated blast furnace slag (GGBS) into a normal hardening hydraulic road binder in accordance with standard EN 13282-2. 10 wt.% addition of BPD as potentially hazardous waste formed during the clinker production by burning alternative fuels was used into cement mixtures. Binder mixtures’ composition was designed according to the recommended sheet of Slovak commercial product (DoroCem). The main constituents of the experimental hydraulic road binder mixture were Portland cement clinker, ground limestone and GGBS. Effect of additional component of BPD on behaviour of fresh mixtures and properties of mortar specimens after 56 days of hardening was studied. The results prove that only one of the tested mixtures (BM2) with the lowest SO3 content and clinker but with the highest amounts of GGBS, ground limestone and alkalis (Na2O + K2O) met the standard requirement for initial setting time for tested fresh mixtures. The higher values of compressive strength found for all hardened specimens are most likely related to the formation of the hydrated and geopolymer products in the binder system. Based on these conclusions, BM2 could be used as hydraulic road binder.
Effects of Hydro Power Plant Releases on Navigation Conditions in the Lower Lock Approach
Authors: Fošumpaur P 
Published: October 2022

Abstract
Waterways are usually designed as multipurpose structures and perform a number of functions in addition to transport. A very common use is the generation of electricity in hydropower plants, water withdrawal for users and flood protection. On canalized rivers, increased attention must be paid to the design of the layout of the individual structures within the barrage. This paper deals with the optimization of barrage layout with respect to the interaction of navigation and hydropower use of the waterway. The navigation conditions below a power plant can be significantly affected by peak operation or by sudden load rejection. These phenomena can generate a negative surge wave with very adverse effects on navigation. In addition, special attention should be paid to the current conditions in the lower lock approach area and the guard wall that separates the lock approach from the weir and the power plant should be optimised. Appropriate design of operational and structural measures can significantly increase the traffic capacity of a waterway. The research was conducted as part of project no. DG18P02OVV004 within a support programme for applied research and experimental development of national and cultural identity, funded by the Ministry of Culture of the Czech Republic.
The possibilities of using digital twin technology in the life cycle of buildings
Authors: Struková Z , Kozlovská M , Tažiková A , Čabala J 
Published: October 2022

Abstract
The article deals with Digital twin (DT) technology, as one of the latest technologies, which, unlike construction, is already widely used in other industries. The theoretical knowledge basis of this technology in construction industry lies in understanding the characteristics of the digital twin and in using knowledge about it from other areas of application. Based on acquired theoretical knowledge and an example from construction practice, the aim of the article is to demonstrate the development process of a digital twin and to indicate the benefits of using Asset Information Model (AIM) as an essential source of information for development of DT during the phases of the life cycle of buildings. The case study analyses the process of development of a digital twin of the heating system in family house, from the initial design of a heating project, through use of 3D scanning to create a virtual model and the integration of various tools of Internet of Things technology to the creation of its virtual model. Reflexion on the benefits of AIM in various phases of the life cycle of buildings, induced from the study of theoretical knowledge and based on the case study, is an essential part of the article.
ELF Density: Extending English as a Lingua Franca Research to Monological ELF Texts and Speeches
Authors: Albl-Mikasa Michaela , Gieshoff Anne Catherine 
Published: October 2022

Abstract
The focus to date on interactive encounters has resulted in there being little research into monological texts and speeches produced in English as a lingua franca (ELF) contexts. They are, however, the very substance of what interpreters and translators increasingly deal with today. The quality of these language professionals’ performance depends to a large extent on the input they receive. From a translation and interpreting studies (TIS) perspective, understanding what makes an ELF text or speech special and different seems paramount. In this paper, we present an innovative approach to exploring what is in an ELF text. We introduce the concepts of ‘ELF density’ and ‘ELF dense spots’ to capture and visualize types and tokens of ELF features as well as clusters they form at a local and more global level across stretches of text or speech and discuss a number of methodological challenges in determining ELF density. Based on a preliminary retrospective verbal protocol and interview analysis following 26 professional interpreters’ renditions, we aim to demonstrate the concepts’ added value for TIS, but also for non-TIS related applied linguistic tasks geared towards ELF.
GAE-LGA: integration of multi-omics data with graph autoencoders to identify lncRNA–PCG associations
Authors: Gao Meihong , Liu Shuhui , Qi Yang , Guo Xinpeng , Shang Xuequn 
Published: October 2022

Abstract
Long non-coding RNAs (lncRNAs) can disrupt the biological functions of protein-coding genes (PCGs) to cause cancer. However, the relationship between lncRNAs and PCGs remains unclear and difficult to predict. Machine learning has achieved a satisfactory performance in association prediction, but to our knowledge, it is currently less used in lncRNA–PCG association prediction. Therefore, we introduce GAE-LGA, a powerful deep learning model with graph autoencoders as components, to recognize potential lncRNA–PCG associations. GAE-LGA jointly explored lncRNA–PCG learning and cross-omics correlation learning for effective lncRNA–PCG association identification. The functional similarity and multi-omics similarity of lncRNAs and PCGs were accumulated and encoded by graph autoencoders to extract feature representations of lncRNAs and PCGs, which were subsequently used for decoding to obtain candidate lncRNA–PCG pairs. Comprehensive evaluation demonstrated that GAE-LGA can successfully capture lncRNA–PCG associations with strong robustness and outperformed other machine learning-based identification methods. Furthermore, multi-omics features were shown to improve the performance of lncRNA–PCG association identification. In conclusion, GAE-LGA can act as an efficient application for lncRNA–PCG association prediction with the following advantages: It fuses multi-omics information into the similarity network, making the feature representation more accurate; it can predict lncRNA–PCG associations for new lncRNAs and identify potential lncRNA–PCG associations with high accuracy.
CysModDB: a comprehensive platform with the integration of manually curated resources and analysis tools for cysteine posttranslational modifications
Authors: Meng Yanzheng , Zhang Lin , Zhang Laizhi , Wang Ziyu , Wang Xuanwen , Li Chan , Chen Yu , Shang Shipeng , Li Lei 
Published: October 2022

Abstract
The unique chemical reactivity of cysteine residues results in various posttranslational modifications (PTMs), which are implicated in regulating a range of fundamental biological processes. With the advent of chemical proteomics technology, thousands of cysteine PTM (CysPTM) sites have been identified from multiple species. A few CysPTM-based databases have been developed, but they mainly focus on data collection rather than various annotations and analytical integration. Here, we present a platform-dubbed CysModDB, integrated with the comprehensive CysPTM resources and analysis tools. CysModDB contains five parts: (1) 70 536 experimentally verified CysPTM sites with annotations of sample origin and enrichment techniques, (2) 21 654 modified proteins annotated with functional regions and structure information, (3) cross-references to external databases such as the protein–protein interactions database, (4) online computational tools for predicting CysPTM sites and (5) integrated analysis tools such as gene enrichment and investigation of sequence features. These parts are integrated using a customized graphic browser and a Basket. The browser uses graphs to represent the distribution of modified sites with different CysPTM types on protein sequences and mapping these sites to the protein structures and functional regions, which assists in exploring cross-talks between the modified sites and their potential effect on protein functions. The Basket connects proteins and CysPTM sites to the analysis tools. In summary, CysModDB is an integrated platform to facilitate the CysPTM research, freely accessible via https://cysmoddb.bioinfogo.org/.
RAS oncogene signal strength regulates matrisomal gene expression and tumorigenicity of mouse keratinocytes
Authors: Cataisson Christophe , Lee Alex J , Zhang Ashley M , Mizes Alicia , Korkmaz Serena , Carofino Brandi L , Meyer Thomas J , Michalowski Aleksandra M , Li Luowei , Yuspa Stuart H 
Published: October 2022

AbstractEnvironmental and molecular carcinogenesis are linked by the discovery that chemical carcinogen induced-mutations in the Hras or Kras genes drives tumor development in mouse skin. Importantly, enhanced expression or allele amplification of the mutant Ras gene contributes to selection of initiated cells, tumor persistence, and progression. To explore the consequences of Ras oncogene signal strength, primary keratinocytes were isolated and cultured from the LSL-HrasG12D and LSL-KrasG12D C57BL/6J mouse models and the mutant allele was activated by adeno-Cre recombinase. Keratinocytes expressing one (H) or two (HH) mutant alleles of HrasG12D, one KrasG12D allele (K), or one of each (HK) were studied. All combinations of activated Ras alleles stimulated proliferation and drove transformation marker expression, but only HH and HK formed tumors. HH, HK, and K sustained long-term keratinocyte growth in vitro, while H and WT could not. RNA-Seq yielded two distinct gene expression profiles; HH, HK, and K formed one cluster while H clustered with WT. Weak MAPK activation was seen in H keratinocytes but treatment with a BRAF inhibitor enhanced MAPK signaling and facilitated tumor formation. K keratinocytes became tumorigenic when they were isolated from mice where the LSL-KrasG12D allele was backcrossed from the C57BL/6 onto the FVB/N background. All tumorigenic keratinocytes but not the non-tumorigenic precursors shared a common remodeling of matrisomal gene expression that is associated with tumor formation. Thus, RAS oncogene signal strength determines cell-autonomous changes in initiated cells that are critical for their tumor-forming potential.
Analytical and Clinical Validation of Cell-Free Circulating Tumor DNA Assay for the Estimation of Tumor Mutational Burden
Authors: Lee Kwang Seob , Seo Jieun , Lee Choong-Kun , Shin Saeam , Choi Zisun , Min Seungki , Yang Jun Hyuek , Kwon Woo Sun , Yun Woobin , Park Mi Ri , Choi Jong Rak , Chung Hyun Cheol , Lee Seung-Tae , Rha Sun Young 
Published: October 2022

Abstract

Background
Ultra-deep sequencing to detect low-frequency mutations in circulating tumor-derived DNA (ctDNA) increases the diagnostic value of liquid biopsy. The demand for large ctDNA panels for comprehensive genomic profiling and tumor mutational burden (TMB) estimation is increasing; however, few ctDNA panels for TMB have been validated. Here, we designed a ctDNA panel with 531 genes, named TMB500, along with a technical and clinical validation.

Methods
Synthetic reference cell-free DNA materials with predefined allele frequencies were sequenced in a total of 92 tests in 6 batches to evaluate the precision, linearity, and limit of detection of the assay. We used clinical samples from 50 patients with various cancers, 11 healthy individuals, and paired tissue samples. Molecular barcoding and data analysis were performed using customized pipelines.

Results
The assay showed high precision and linearity (coefficient of determination, r2 =0.87) for all single nucleotide variants, with a limit of detection of 0.24%. In clinical samples, the TMB500 ctDNA assay detected most variants present and absent in tissues, showing that ctDNA could assess tumor heterogeneity in different tissues and metastasis sites. The estimated TMBs correlated well between tissue and blood, except in 4 cases with extreme heterogeneity that showed very high blood TMBs compared to tissue TMBs. A pilot evaluation showed that the TMB500 assay could be used for disease monitoring.

Conclusions
The TMB500 assay is an accurate and reliable ctDNA assay for many clinical purposes. It may be useful for guiding the treatment of cancers with diverse genomic profiles, estimating TMB in immune therapy, and disease monitoring.
Higher power short duration vs. lower power longer duration posterior wall ablation for atrial fibrillation and oesophageal injury outcomes: a prospective multi-centre randomized controlled study (Hi-Lo HEAT trial)
Authors: Chieng David , Segan Louise , Sugumar Hariharan , Al-Kaisey Ahmed , Hawson Joshua , Moore Benjamin M , Nam Michael C Y , Voskoboinik Aleksandr , Prabhu Sandeep , Ling Liang-Han , Ng Jer Fuu , Brown Gregor , Lee Geoffrey , Morton Joseph , Debinski Henry , Kalman Jonathan M , Kistler Peter M 
Published: October 2022

AbstractAimsRadiofrequency (RF) ablation for pulmonary vein isolation (PVI) in atrial fibrillation (AF) is associated with the risk of oesophageal thermal injury (ETI). Higher power short duration (HPSD) ablation results in preferential local resistive heating over distal conductive heating. Although HPSD has become increasingly common, no randomized study has compared ETI risk with conventional lower power longer duration (LPLD) ablation. This study aims to compare HPSD vs. LPLD ablation on ETI risk.Methods and resultsEighty-eight patients were randomized 1:1 to HPSD or LPLD posterior wall (PW) ablation. Posterior wall ablation was 40 W (HPSD group) or 25 W (LPLD group), with target AI (ablation index) 400/LSI (lesion size index) 4. Anterior wall ablation was 40–50 W, with a target AI 500–550/LSI 5–5.5. Endoscopy was performed on Day 1. The primary endpoint was ETI incidence. The mean age was 61 ± 9 years (31% females). The incidence of ETI (superficial ulcers n = 4) was 4.5%, with equal occurrence in HPSD and LPLD (P = 1.0). There was no difference in the median value of maximal oesophageal temperature (HPSD 38.6°C vs. LPLD 38.7°C, P = 0.43), or the median number of lesions per patient with temperature rise above 39°C (HPSD 1.5 vs. LPLD 2, P = 0.93). Radiofrequency ablation time (23.8 vs. 29.7 min, P &lt; 0.01), PVI duration (46.5 vs. 59 min, P = 0.01), and procedure duration (133 vs. 150 min, P = 0.05) were reduced in HPSD. After a median follow-up of 12 months, AF recurrence was lower in HPSD (15.9% vs. LPLD 34.1%; hazard ratio 0.42, log-rank P = 0.04).ConclusionHigher power short duration ablation was associated with similarly low rates of ETI and shorter total/PVI RF ablation times when compared with LPLD ablation. Higher power short duration ablation is a safe and efficacious approach to PVI.
Multiple solutions at the genomic level in response to selective breeding for high locomotor activity
Authors: Hillis David A , Garland Theodore 
Published: October 2022

Abstract
Replicate lines under uniform selection often evolve in different ways. Previously, analyses using whole-genome sequence data for individual mice (Mus musculus) from 4 replicate High Runner lines and 4 nonselected control lines demonstrated genomic regions that have responded consistently to selection for voluntary wheel-running behavior. Here, we ask whether the High Runner lines have evolved differently from each other, even though they reached selection limits at similar levels. We focus on 1 High Runner line (HR3) that became fixed for a mutation at a gene of major effect (Myh4Minimsc) that, in the homozygous condition, causes a 50% reduction in hindlimb muscle mass and many pleiotropic effects. We excluded HR3 from SNP analyses and identified 19 regions not consistently identified in analyses with all 4 lines. Repeating analyses while dropping each of the other High Runner lines identified 12, 8, and 6 such regions. (Of these 45 regions, 37 were unique.) These results suggest that each High Runner line indeed responded to selection somewhat uniquely, but also that HR3 is the most distinct. We then applied 2 additional analytical approaches when dropping HR3 only (based on haplotypes and nonstatistical tests involving fixation patterns). All 3 approaches identified 7 new regions (as compared with analyses using all 4 High Runner lines) that include genes associated with activity levels, dopamine signaling, hippocampus morphology, heart size, and body size, all of which differ between High Runner and control lines. Our results illustrate how multiple solutions and “private” alleles can obscure general signatures of selection involving “public” alleles.
Children of the Gulag, Long Road to Justice: The Challenges and Limitations of Reparations in Russia
Authors: Durdiyeva Selbi 
Published: October 2022

ABSTRACT∞
The article examines the 2019 judgment of the Constitutional Court of Russia concerning the right to compensation of housing and right to return of the children of the victims deported by the Soviet state to the gulag or exile. Despite the favourable judgment of the Constitutional Court, the right to remedy for the victims was still unattainable. The article examines why this was the case by drawing on the absence of internal and external coherence of reparations programmes, the approach to reparations throughout history in the Soviet Union and Russia, the Law on Rehabilitation and other factors that rendered the reparations unobtainable. The article argues that in the absence of holistic transitional justice measures and accountability for crimes, separate laws and court judgments have been unable to deliver justice, resulting in a long, cumbersome and often humiliating and fruitless process for victims attempting to claim their rights.
Ana posteriorierror estimator for isogeometric analysis on trimmed geometries
Authors: Buffa Annalisa , Chanon Ondine , Vázquez Rafael 
Published: October 2022

AbstractTrimming consists of cutting away parts of a geometric domain, without reconstructing a global parametrization (meshing). It is a widely used operation in computer-aided design, which generates meshes that are unfitted with the described physical object. This paper develops an adaptive mesh refinement strategy on trimmed geometries in the context of hierarchical B-spline-based isogeometric analysis. A residual a posteriori estimator of the energy norm of the numerical approximation error is derived, in the context of the Poisson equation. The estimator is proven to be reliable, independently of the number of hierarchical levels and of the way the trimmed boundaries cut the underlying mesh. Numerical experiments are performed to validate the presented theory, and to show that the estimator’s effectivity index is independent of the size of the active part of the trimmed mesh elements.
Backstage Mockery: Impoliteness and Asymmetry on the World Stage
Authors: Van Rythoven Eric 
Published: October 2022

AbstractIn recent years, episodes of diplomatic “impoliteness” have attracted growing attention among international relations (IR) scholars. Whether in the form of sharp-edged humor, insults, or other face-undermining behavior, scholars are increasingly examining episodes where the expected civility of diplomacy breaks down and impoliteness becomes weaponized as a tool of statecraft. While sympathetic to these approaches, I argue that the deployment of impoliteness cannot be understood in isolation from the powerful asymmetries that shape global politics. To show why, I advance a theory of mockery in international politics with an emphasis of a specific kind: backstage mockery. Building on the work of Goffman, I conceptualize mocking displays as acts of ridicule that undermine an actor's positive public image or “face” based on two contextual factors: (1) perceptions of transgression and (2) relative status. In hierarchical settings, lower-status members are often unable to openly criticize the transgressions of their social superiors because of the threat of retaliation. Covert or “backstage” mockery offers an outlet for weaker members to express their concerns and build solidarity with other members, while at the same time indirectly signaling those with higher status about problems. To illustrate the significance of backstage mockery, I discuss Canada–US relations and the 2019 viral video of NATO leaders appearing to privately mock US President Donald Trump.