bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of lncRNA-miRNA-mRNA regulatory network and correlation between prognostic biomarker PDGFRB and immune infiltrates in stomach adenocarcinoma
Authors: Yang Guang , Wu Ying , Zheng yi Ru , Jin shun Zai , Yan Bin 
Published: February 2022

Abstract
Purpose: Gastric cancer is a highly malignant and heterogeneous tumour with a poor prognosis and a high recurrence rate. Overexpressed PDGFRB has a correlation with poor survival outcomes in several malignancies. The objective of our study was to evaluate the prognostic value of PDGFRB and identify a lncRNA-miRNA-mRNA ceRNA network involved in stomach adenocarcinoma.Methods: RNA-sequencing data, miRNA-sequencing data and corresponding patient clinical information were collected from the TCGA database. All statistical analysis was performed through the edgeR package. ENCORI database was applied to screen out miRNAs and lncRNAs interacting with the target gene. A lncRNA-miRNA-mRNA ceRNA network was constructed through Cytoscape. For DEmiRNAs, DElncRNAs and mRNA，the differences between the overall survival of high-expression and low-expression group were evaluated by R language. TIMER 2.0 and GEPIA database were used to evaluate the value of PDGFRB in immune cell infiltration and immunotherapy. Results: Compared with normal tissue samples, PDGFRB was significantly upregulated in stomach adenocarcinoma. The upregulated expression of PDGFRB was significantly related to histologic grade, topography and clinical stage. LncRNA CASC15 and has-miR-30e-5p were identified to have correlations with PDGFRB. Patients with an elevated PDGFRB, lncRNA CASC15 and a decreased has-miR-30e-5p had a poor prognosis, in which elevated PDGFRB was an independent poor prognostic factor. In addition, the positive associations among PDGFRB, tumor infiltrating immune cells and immunotherapy were also observed. Conclusions: An increased PDGFRB may serve as a promising and potential independent prognostic biomarker of a poor survival and therapeutic targets in stomach adenocarcinoma.
Effect of Residual Alkali Level in Softwood Kraft Cooking
Authors: Brännvall Elisabet , Norberg Lars , Karlström Katarina 
Published: February 2022

Abstract
In washing of softwood pulp, pH is reduced to such low levels that re-precipitation of dissolved lignin may result. It was hypothesized that the lower the residual alkali after cooking, the higher the risk of lignin re-precipitation, which in turn would affect the subsequent oxygen delignification stage negatively. To test the hypothesis, kraft cooks were performed to different residual alkali levels, ranging from 5 to 15 g/l and the delignified chips were subjected to different washing strategies and then oxygen delignified. However, the results show that even at low residual alkali, lignin stays in solution and only minor amounts of lignin re-precipitate during washing. The amount was irrespective of the residual alkali level in the cook and amounted to 1-2 kappa number units. No effect of residual alkali level was observed on the performance of the oxygen delignification stage. The residual alkali level affected the lignin concentration in the lumen after cooking. At the low residual alkali level, the lignin concentration in the lumen liquor was equal to the free liquor and as the residual alkali level increased, the lignin concentration in the lumen increased, while the lignin concentration in the free black liquor remained on the same level at all residual alkali levels.
Improving hypertension awareness and management in Vietnam through a community-based model
Authors: McGuire Helen , Van Truong Bui , Tu Hien Le Thi , Thanh Huyen Nguyen , Murray Marge , Shellaby Jason , Aerts Ann , George Roshini , Hodges Mary 
Published: February 2022

Abstract
Hypertension prevalence in Vietnam is high, but few people are aware of their disease status. Detection, diagnosis, and treatment are limited, so new approaches are needed to improve awareness and manage the condition, especially at the primary health care level. The Communities for Healthy Hearts programme operated in four districts of Ho Chi Minh City, with the aim of increasing awareness and ensuring linkage to care. Interventions focused on extending services beyond health facilities to convenient community locations; training cadres of community volunteers to screen, refer, and follow up with clients; training health workers in facilities to diagnose, educate, and manage patients referred from the community; and supporting the process with digital case tracking. Community-based blood pressure screenings took place in client homes, commune health stations, and non-traditional sites such as coffee shops, dentists’ offices, marketplaces, neighbourhood watch/security posts, and tailor shops. In total, 121,273 adults aged 40 and older were screened. Of these, 25.1% had elevated blood pressure or a previous hypertension diagnosis and were referred to health facilities. Of those referred, 56.2% were confirmed to have hypertension, and of those confirmed, 75.2% were treated. Of those treated, 51.0% achieved blood pressure control, a favourable outcome over past programmes.
The role of routine laboratory tests after unilateral total knee arthroplasty
Authors: Li An an , Zhang Yu , zhang Hao , xiao Shi-ning , long xin-hua , Wang Shi-jiang , zhou yang 
Published: February 2022

Abstract
BackgroundRecent studies suggest that routine laboratory tests are not required within 1 day after partial knee arthroplasty. This study aimed to evaluate the utility of routine postoperative laboratory tests after initial unilateral total knee arthroplasty(TKA) in an Asian population and to identify risk factors associated with abnormal test results.MethodsThe clinical data of patients who underwent original unilateral TKA from 2015 to 2020 were retrospectively analyzed. Patient characteristics and laboratory test values were recorded. Multivariate binary logistic regression analysis was also performed to identify risk factors associated with 3 abnormal laboratory values.ResultsA total of 713 patients were included in this study and underwent relevant laboratory tests within 3 days of TKA surgery. However, only a small proportion of patients with anemia (8.1 %), hypoalbuminemia (9.9 %), and abnormal serum potassium levels (3.4 %) required clinical intervention after surgery. Binary logistic regression analysis revealed that independent risk factors for postoperative blood transfusion in TKA patients included preoperative hemoglobin level, estimated blood loss, and age. Related risk factors for postoperative albumin supplementation were preoperative albumin level, intraoperative blood loss and operation time. In addition, lower body mass index (BMI) and preoperative hypokalemia were found to be potential risk factors for postoperative potassium supplementation.ConclusionSince more than 90% of abnormal postoperative laboratory tests do not require clinical intervention, the authors believe that it is of little significance for patients with primary unilateral TKA to receive routine laboratory tests after surgery. However, in patients with established risk factors, postoperative laboratory testing is still necessary.
A study of libration points and analysis of their stability behavior in-ring body problem under albedo effect
Authors: Kumar Manoj , Kushvah Badam Singh 
Published: February 2022

Abstract
Our intention in this article is to analyze the fundamental analysis of the zones and their existing libration points under the solar radiation pressure and albedo effects. We can feel about albedos at any Nature’s property wherever the Sun rays exist. As we know that important phase of the dynamical system of any celestial body is its stability and the factors which change its behavior of motion. This research article presents an explicit analysis of the albedo effect for ring body problems for a specific value of v = 6, which explains the behavior of existing zone’s stability, shifting of their libration points with monotonicity and the characteristic roots of δ confirms the behavior of stability condition for existing zones as well as their existing libration points in a synodic frame of references. We observed that in this problem from an experimental point of view, a little change in v, changes the complete behavior of the problem and leaves a nice platform of the proof. In this problem, β plays a significant role, which affects firstly the whole dynamics of peripheral primaries, the mass parameters β = 0 and β ≠ 0 generally confirm that this synodic system wears, an absence of a center of mass and the presence of the center of mass, respectively. We have observed in this article is the libration point L0, which exists in the center and has peripheral primary P0, remains unchanged throughout applying the albedo effect. The stability analysis confirms the location of the libration points, whether it is stable, unstable, etc., but here we have seen the more interesting things i.e., most of the locations of the libration points except exceptionals are found unstable. This article also shows the momentum of the position of all the libration points with their existing zone as well as peripheral primaries, by the effect of the radiation pressure and albedo. It is also analyzed here that this complete problem works in a certain short and very sensitive range set (−1.75, 1.75) because this problem is filled with trigonometric periodic functions. In this problem, we have considered only the real roots of the axis x, y, whereas imaginary roots are not considered. For stability analysis, we have taken both real and complex roots of δ. Results of this article for v = 6 may help us to analyze an imaginary behavior of our solar system’s planets in a blank paper, provided by certain rules and regulations by equations of motion because real behavior may be far away from us. By the present study, it is found that the whole behavior of the system for various v whenever change, which can be extended later in another way.
Cancer immunohistogram prediction of the efficacy of immune checkpoint inhibitors in urological cancer patients: A preliminary study
Authors: Kijima Toshiki , Kubo Terufumi , Nishihara Daisaku , Nukui Akinori , Hirohashi Yoshihiko , Torigoe Toshihiko , Kamai Takao 
Published: February 2022

Abstract
We developed an immunohistogram representing an individual cancer-immunity cycle based on immunohistochemical analyses. We evaluated its predictive ability for immune checkpoint inhibitors (ICI) efficacy in 11 patients with urothelial carcinoma and 7 patients with renal cell carcinoma who underwent surgery and received ICIs for disease recurrence. Immunohistochemical analyses for CD8, TIA-1, HLA class I, HLA-DR, and PD-L1 were performed and scored 0–3. T-cell infiltration pattern was classified into desert, excluded, partially inflamed, and inflamed. Tumors with an inflamed or partially inflamed pattern and positive scores (score ≥ 1) for all five immune markers were classified as “immune-hot” and others were classified as “immune-cold”. Association between the immunohistogram and ICI treatment efficacy was evaluated with objective response rates, disease control rate (DCR), progression-free survival (PFS), and cancer-specific survival (CSS). Eight (44%) and 10 (56%) of patients had immune-hot and immune-cold tumors, respectively. Immune-hot tumors showed higher DCR (100% vs. 40%, p < 0.01), longer PFS (median unreached for hot, 1.3 months for cold, p < 0.01), and longer CSS (median unreached for hot, 3.3 months for cold, p = 0.04) than immune-cold tumors. The immunohistogram could be clinically useful as an accessible biomarker for precision cancer immunotherapy in urological cancer.
Expanding the Scope of Emergency IPV Sheltering: Moral Entrepreneurs and the Co-Sheltering of Companion Animals
Authors: Lindsay Sarah May 
Published: February 2022

Abstract
Purpose: Companion animals are rarely co-sheltered in intimate partner violence (IPV) shelters in Canada, despite their common consideration as family, and an awareness of the “the Link” between human and nonhuman abuse. This article reports on the few IPV facilities in Ontario who do house their clients’ companion animals and seeks to identify how co-sheltering is emerging as part of the evolution of IPV sheltering. Methods: This study is part of a larger research project, which utilizes semi-structured interviews with Ontario IPV shelter workers and volunteers to assess how companion animals fit in these facilities. Guided by the principles of action research, these conversations were textually analyzed for themes and sentiments regarding co-sheltering. This paper reports on co-shelter facilities and practices present in the larger sample. Results:We find that co-sheltering is a grassroots action undertaken by moral entrepreneurs who engage in covert and overt actions to expand the circle of care in these spaces. These actions are also found to be in response to the ongoing social problem of gendered violence; these moral entrepreneurs follow in the footsteps of those who began to organize in the 1970s from a feminist standpoint, addressing a gap in shelter and safety for women affected by this social problem. Conclusions: We find that the provision of co-sheltering in IPV facilities appears to depend solely on the action of shelter workers “on-the-ground”, who identify an urgent need to expand the scope of care in their shelter to include clients’ companion animals. More research is needed to learn about IPV co-shelters elsewhere in the country, and to study the impact of this inclusive housing option on the lives of clients, workers, and companion animals.
Potential Risks of Agricultural Intensification in Tropical Catchments: the Case of Lake Naivasha
Authors: Onyango Joel , Kitaka Nzula , Bruggen J. J.A. Van , Irvine Kenneth , Simaika John 
Published: February 2022

Abstract
The use of both fertilizers and pesticides in the Lake Naivasha Catchment, is associated with agricultural intensification and has resulted in enrichment of aquatic ecosystems with nutrients, coupled with exposure of aquatic biota to pesticide residues affecting aquatic ecosystem structure and function. This study explored the changes in land use and related it with the concentrations of nutrients and selected pesticides, to show the potential of combined (nutrients and pesticides) risks associated with agricultural intensification in Lake Naivasha catchment, a tropical catchment in Kenya. The results indicate that between 1989 and 2019 there was an increase of cropland by 623 km2, a reduction of forest cover by 200 km2, increase of grasslands by 534 km2, a reduction in bare soils by 100 km2, and an increase of 540 km2 of built area. The land cover changes were correlated with increased concentrations of nutrients and pesticides in surface waters across sampling sites monitored in 2015. The results further indicated that while the concentrations of nitrogen and phosphorus indicated a potential stoichiometric nitrogen limitation, increase in forest cover resulted in decreased nutrient concentrations in the river water. Concentrations of DDT and technical HCH active ingredients indicated significant relationships between land use changes and intensification. Areas with extensive farming systems were associated with higher concentrations of TP and ∑DDT compared with areas of high intensification, which may reflect high connectivity between intensive agricultural systems and river ecosystems. When combined nutrients and pesticides were considered, most of the lower reaches of the rivers draining to L. Naivasha were of poor water quality status as defined by Kenyan water quality regulations. The changes in land cover and agricultural intensification in the Naivasha catchment needs management and policy frameworks that manage the combined contamination from nutrients and pesticide residues. Enhanced investment in institutional and policy reforms, and an integrated review of water quality standards and monitoring indicators that combine biological, physical, and chemical monitoring, is necessary for future management of the catchment.
The association of growth differentiation factor-15 levels and osteoporosis in patients with thalassemia
Authors: Teawtrakul Nattiya , Chansai Siriyakorn , Yamsri Supawadee , Chansung Kanchana , wanitpongpun Chinadol , Lanamtieng Theerin , Phiphitaporn Pisa , Fucharoen Supan , Pongchaiyakul Chatlert 
Published: February 2022

Abstract
Introduction: Ineffective erythropoiesis is a significant risk factor for osteoporosis in individuals with thalassemia. Growth differentiation factor-15 (GDF15), a biomarker of ineffective erythropoiesis, has previously been found to be elevated in thalassemia patients. The purpose of this study was to examine the association between GDF15 levels and osteoporosis in patients with thalassemia. Methods: A cross-sectional study was conducted in 130 adult patients with thalassemia at Srinagarind Hospital, Thailand. Bone mineral density (BMD) at the lumbar spine was evaluated by dual-energy X-ray absorptiometry (DXA), and with a Z-score of less than -2.0 SD was defined as osteoporosis. GDF-15 was measured using the enzyme-linked immunosorbent assay (ELISA). Logistic regression analysis was used to examine the associated factors with the development of osteoporosis. Receiver operator characteristic (ROC) curve analysis was used to estimate the threshold of GDF15 in predicting osteoporosis.Results: Osteoporosis was detected in 55.4% (72/130) of the patients. Advanced age and high GDF15 levels were positively associated with osteoporosis, while an increased hemoglobin level was negatively associated with osteoporosis in patients with thalassemia. In this study, the GDF15 level’s ROC demonstrated a good performance in predicting osteoporosis (AUC=0.77). Using a cut-off point of GDF15 ≥ 20,000 pg/mL, the sensitivity and specificity were 90.2% and 60.3%.Conclusions: The prevalence of osteoporosis is high among adult thalassemia patients. Age and high GDF15 levels were significantly associated with osteoporosis in this study. A higher hemoglobin level is associated with a lower risk of osteoporosis. The findings of this study suggest that GDF15 could be used as a predictive biomarker for osteoporosis in patients with thalassemia. Adequate red blood cell transfusions and suppression of GDF15 function may be beneficial in preventing or delaying osteoporosis progression.
Altered Temporal-Parietal Morphological Similarity Networks in Non-Small Cell Lung Cancer Patients Following Chemotherapy: An MRI Preliminary Study
Authors: Chen Gong , Wu Chuan , Liu Yuan , Fang Zengyi , Luo Liping , Lai Xin , Wang Weidong , Dong Li 
Published: February 2022

Abstract
Non-small cell lung cancer (NSCLC) accounts for more than 85% of all lung cancer cases, and chemotherapy-related brain changes (known as “chemobrain”) in NSCLC patients were found in previous studies. However, the effects of platinum-based chemotherapy on the brain structural networks are still unclear. Structural magnetic resonance imaging (sMRI) data were collected from 32 NSCLC patients following platinum-based chemotherapy, 36 NSCLC patients without chemotherapy, and 39 healthy controls. Clinical physiological indicators of NSCLC patients were collected. Then, morphological similarity networks were constructed using individual MRI data, and topological properties were calculated using the graph theory method. Differences between the three groups were investigated using one-way ANOVA and two-sample t-test, and relations between the topological properties and clinical physiological indicators were calculated. We found that degree (F(2,104) = 18.367, p < 0.001) and nodal efficiency (F(2,104) = 12.546, p < 0.001) in temporal-parietal networks were significantly reduced in NSCLC patients following platinum-based chemotherapy compared to healthy controls/patients without chemotherapy. These changes (p < 0.05) were positively correlated with clinical measures, including thrombocytes, granulocytes and hemoglobin, and were negatively correlated with measures of triglycerides and cholesterol levels. Network properties including clustering coefficient (F(2,104) = 41.435, p < 0.001), number of K-edges (F(2,104) = 40.304, p < 0.001), density of K-edges (F(2,104) = 40.304, p < 0.001), global efficiency (F(2,104) = 42.585, p < 0.001) and small-world (F(2,104) = 37.132, p < 0.001) were also significantly reduced. These results indicate that platinum-based chemotherapy might cause cerebrovascular damage and clinical indicators’ changes, which then cause the properties of morphological similarity networks’ changes in the temporal and parietal lobes. This study may help us better understand the “chemobrain” in NSCLC patients.
Long Noncoding RNA PVT1 Predicts Poor Prognosis and Promotes the Progression of Colorectal Cancer Through the MiR-24-3p/NRP1 Axis in Zebrafish Xenografts
Authors: Yin Hailin , Gu Shanye , Li Guiqin , Yu Hanxu , Zhang Xishan , Zuo Yangsong 
Published: February 2022

Abstract
BackgroundLong noncoding RNAs (lncRNAs) play important roles in the progression of human cancer. It is reported lncRNA plasmacytoma variant translocation 1 (PVT1) is involved in colorectal cancer (CRC), however, the underlying mechanism remains to be explored deeply, especially by in vivo models.MethodsThe expression level of PVT1 in CRC tissues was analyzed in TCGA and GEO database. The prognosis of PVT1 in CRC was assessed by analyzing the correlation between the expression level of PVT1 and overall survival in CRC patients by Kaplan-Meier survival analysis. The CCK-8 and colony formation assays were performed to study the proliferation of CRC cells, and transwell assays was used to assess the migration of CRC cells in vitro. The targeting relationships among PVT1, miR-24-3p and Neuropilin 1 (NRP1) was predicted by StarBase, and examined by dual-luciferase reporter assays. The expression levels of PVT1, miR-24-3p and NRP1 were quantified by quantitative real time PCR and/or western blotting. The zebrafish CRC xenograft models were used to study the function of PVT1, miR-24-3p and NRP1 in vivo.ResultsBioinformatics analysis showed that PVT1 was upregulated in CRC tissues and highly associated with poor prognosis. Knockdown of PVT1 inhibited cell proliferation and migration of CRC cells, while overexpression of PVT1 promoted the progression of CRC cells in vitro. In zebrafish xenografts, silencing of PVT1 also suppressed the growth and metastasis of CRC cells. miR-24-3p, binding with PVT1, negatively regulated the progression of CRC cells. Inhibition of miR-24-3p counteracted the effects of PVT1 knockdown. In addition, miR-24-3p could regulate CRC progression by targeting NRP1 directly. Moreover, the expression of NRP1 was regulated by PVT1, and NRP1 overexpression could partially rescue the inhibition effects of PVT1 knockdown on CRC progression in vitro and in vivo.ConclusionsOur study reveals that PVT1 promoted the proliferation and metastasis of CRC via regulating miR-24-3p/NRP1 axis, and it could be a prognosis biomarker of CRC. This study provides a potential therapeutic target for CRC patients.