bluuebunny/crossref_metadata_2025_split huggingface dataset:
TLC599 in patients with osteoarthritis of the knee: a phase IIa, randomized, placebo-controlled, dose-finding study
Authors: Hunter David J. , Chang Chi-Ching , Wei James Cheng-Chung , Lin Hsiao-Yi , Brown Carl , Tai Tien-Tzu , Wu Chih-Feng , Chuang Wing Chia-Ming , Shih Sheue-Fang 
Published: February 2022

Abstract
Background
Corticosteroid injection for knee osteoarthritis is limited by its modest duration of treatment effect. The liposome formulation of dexamethasone sodium phosphate (TLC599) was developed for the sustained relief of osteoarthritis pain. This clinical study was conducted to evaluate the efficacy and safety of TLC599 at two dose levels in patients with knee osteoarthritis.

Methods
A randomized, double-blinded, placebo-controlled study was conducted in 75 patients with osteoarthritis of the knee from 13 study centers. Patients were randomized and administered a single intra-articular injection of TLC599 or placebo and assessed for efficacy and safety for 24 weeks. Patient-reported outcomes included the Western Ontario and McMaster Universities Arthritis (WOMAC) Index for pain and function and visual analog scale for pain.

Results
TLC599 at 12 mg demonstrated significantly greater reduction in WOMAC pain through 12 weeks (least squares (LS) mean difference = − 0.37, p = 0.0027) and through 24 weeks (LS mean difference = − 0.35, p = 0.0037) when compared to placebo. TLC599 12 mg also exhibited significantly greater improvement in function when compared to placebo at 24 weeks (LS mean difference = − 0.26, p = 0.0457). TLC599 18 mg did not significantly improve pain or function in comparison with placebo. The use of acetaminophen during the study was less in both TLC599 groups in comparison with placebo. No major or unexpected safety issues were reported.

Conclusions
In participants with symptomatic knee osteoarthritis, TLC599 is a well-tolerated treatment that reduces pain and improves function for up to 24 weeks, a longer duration than that reported for existing IA treatments.

Trial registration
ClinicalTrials.gov, NCT03005873. Registered on 29 December 2016
Assessment of men involvement in family planning services use and associated factors in rural Ghana
Authors: Kwawukume Senanu Abigail Kpekpo , Laar Alexander Suuk , Abdulai Tanko 
Published: February 2022

Abstract
Background
In low-and-middle income countries (LMICs) less attention is paid to men’s involvement in Family Planning (FP) programs where public health officials have advocated the involvement of men as a strategy for addressing the dismal performance of FP programs. The study assessed the involvement of men in FP use and the factors which promote or hinder the uptake of FP services among partners in a rural setting of northern Ghana.

Methods
A cross-sectional descriptive study was used to collect data from 200 respondents. Study respondents were selected through random cluster sampling.

Results
The findings showed that male partners’ knowledge (95.5%) and approval (72.8%) of FP services were high. About 48% of men were involved in FP service utilization. Having living children (aOR; 1.71(1.27, 2.15)) and being knowledgeable (aOR; 6.14(1.38, 10.90)) about FP were positively associated men’s involvement in FP service utilization. The findings also indicated that men had a higher propensity (X2 = 4.5534, p = 0.033) of supporting a FP method use. Women who reported that their spouse supported FP method use were more likely to use a contraceptive method (X2 = 9.5223, P = 0.002) if their spouse supported FP method use (X2 = 9.5223, P = 0.002) and if their partners had some education (X2 = 14.1133, P = 0.000). Reasons for low contraceptive use were health risks, side effects, and socio-cultural norms.

Conclusion
Family planning programs need to include men at all levels of health promotion and education of FP programs to help reduce misconceptions about contraceptive methods to increase acceptance and use among partners in rural settings of Ghana.
The role of micro, small and medium enterprises (MSMEs) to the sustainable development of sub-Saharan Africa and its challenges: a systematic review of evidence from Ethiopia
Authors: Endris Ebrahim , Kassegn Andualem 
Published: February 2022

AbstractMicro, small and medium-sized enterprises (MSMEs) have a potential impact on achieving many of the sustainable development goals much greater than their size. This review aimed to investigate existing literature on the contribution of MSMEs to the sustainable development of Ethiopia and its challenges. The review provides a comprehensive and systematic summary of evidence and provides future research directions. A systematic review methodology was adopted through reviewing the available literature comprehensively including research articles, policy documents, and reports over the period 2011–2021 from ScienceDirect, Google Scholar, ECONBIZ, IJSTOR, EBSCO, Web of Science, and Scopus databases. A search on these databases and grey literature returned 1270 articles; 87 papers were included in this review following screening of aticles using pre-determined criteria. The paper found that MSMEs significantly contributed to the sustainable development goals of Ethiopia through creating employment, alleviating poverty, and improving their living standards. However, the review has identified access to finance, access to electricity, and trade regulation are the major constraints for the development of the sector. The review outlines key policy implications to develop a comprehensive policy that alleviates the existing challenges of the sector and calls for further MSMEs impact evaluation research.
Development of a rational framework for the therapeutic efficacy of fecal microbiota transplantation for calf diarrhea treatment
Authors: Islam Jahidul , Tanimizu Masae , Shimizu Yu , Goto Yoshiaki , Ohtani Natsuki , Sugiyama Kentaro , Tatezaki Eriko , Sato Masumi , Makino Eiji , Shimada Toru , Ueda Chise , Matsuo Ayumi , Suyama Yoshihisa , Sakai Yoshifumi , Furukawa Mutsumi , Usami Katsuki , Yoneyama Hiroshi , Aso Hisashi , Tanaka Hidekazu , Nochi Tomonori 
Published: February 2022

AbstractBackgroundEstablishing fecal microbiota transplantation (FMT) to prevent multifactorial diarrhea in calves is challenging because of the differences in farm management practices, the lack of optimal donors, and recipient selection. In this study, the underlying factors of successful and unsuccessful FMT treatment cases are elucidated, and the potential markers for predicting successful FMT are identified using fecal metagenomics via 16S rRNA gene sequencing, fecal metabolomics via capillary electrophoresis time-of-flight mass spectrometry, and machine learning approaches.ResultsSpecifically, 20 FMT treatment cases, in which feces from healthy donors were intrarectally transferred into recipient diarrheal calves, were conducted with a success rate of 70%.Selenomonaswas identified as a microorganism genus that showed significant donor–recipient compatibility in successful FMT treatments. A strong positive correlation between the microbiome and metabolome data, which is a prerequisite factor for FMT success, was confirmed by Procrustes analysis in successful FMT (r= 0.7439,P= 0.0001). Additionally, weighted gene correlation network analysis confirmed the positively or negatively correlated pairs of bacterial taxa (familyVeillonellaceae) and metabolomic features (i.e., amino acids and short-chain fatty acids) responsible for FMT success. Further analysis aimed at establishing criteria for donor selection identified the genusSporobacteras a potential biomarker in successful donor selection. Low levels of metabolites, such as glycerol 3-phosphate, dihydroxyacetone phosphate, and isoamylamine, in the donor or recipients prior to FMT, are predicted to facilitate FMT.ConclusionsOverall, we provide the first substantial evidence of the factors related to FMT success or failure; these findings could improve the design of future microbial therapeutics for treating diarrhea in calves.
Effects of hyperinsulinemia on pancreatic cancer development and the immune microenvironment revealed through single-cell transcriptomics
Authors: Zhang Anni M. Y. , Chu Ken H. , Daly Brian F. , Ruiter Titine , Dou Yan , Yang Jenny C. C. , de Winter Twan J. J. , Chhuor Justin , Wang Su , Flibotte Stephane , Zhao Yiwei Bernie , Hu Xiaoke , Li Hong , Rideout Elizabeth J. , Schaeffer David F. , Johnson James D. , Kopp Janel L. 
Published: February 2022

Abstract
Background
Hyperinsulinemia is independently associated with increased risk and mortality of pancreatic cancer. We recently reported that genetically reduced insulin production resulted in ~ 50% suppression of pancreatic intraepithelial neoplasia (PanIN) precancerous lesions in mice. However, only female mice remained normoglycemic, and only the gene dosage of the rodent-specific Ins1 alleles was tested in our previous model. Moreover, we did not delve into the molecular and cellular mechanisms associated with modulating hyperinsulinemia.

Methods
We studied how reduced Ins2 gene dosage affects PanIN lesion development in both male and female Ptf1aCreER;KrasLSL-G12D mice lacking the rodent-specific Ins1 gene (Ins1-/-). We generated control mice having two alleles of the wild-type Ins2 gene (Ptf1aCreER;KrasLSL-G12D;Ins1-/-;Ins2+/+) and experimental mice having one allele of Ins2 gene (Ptf1aCreER;KrasLSL-G12D;Ins1-/-;Ins2+/-). We then performed thorough histopathological analyses and single-cell transcriptomics for both genotypes and sexes.

Results
High-fat diet–induced hyperinsulinemia was transiently or modestly reduced in female and male mice, respectively, with only one allele of Ins2. This occurred without dramatically affecting glucose tolerance. Genetic reduction of insulin production resulted in mice with a tendency for less PanIN and acinar-to-ductal metaplasia (ADM) lesions. Using single-cell transcriptomics, we found hyperinsulinemia affected multiple cell types in the pancreas, with the most statistically significant effects on local immune cell types that were highly represented in our sampled cell population. Specifically, hyperinsulinemia modulated pathways associated with protein translation, MAPK-ERK signaling, and PI3K-AKT signaling, which were changed in epithelial cells and subsets of immune cells.

Conclusions
These data suggest a potential role for the immune microenvironment in hyperinsulinemia-driven PanIN development. Together with our previous work, we propose that mild suppression of insulin levels may be useful in preventing pancreatic cancer by acting on multiple cell types.
Testing the psychometric properties of a Chinese version of Dyadic Relationship Scale for families of people with hypertension in China
Authors: Zeng Dejian , Yang Chen , Chien Wai Tong 
Published: February 2022

Abstract
Background
Interventions for people with chronic illness have increasingly got involvement and partnership with family members in China and worldwide. The patient-family interactions in chronic illness care can greatly influence not only family dyadic relationship or collaboration in caregiving but also both patient’s and caregiver’s health and well-being. To date, very few instruments have been developed to measure the family dyadic relationship; and none has been found in Chinese language. This study aimed to translate the original English Dyadic Relationship Scales (DRS), including DRS-patient and DRS-caregiver, into simplified Chinese language (DRS-C) and examine their psychometric properties in Chinese people with hypertension in a rural community.

Methods
The Brislin’s model of translation was adopted for translation. Face and content validity and semantic equivalence of the translated Chinese version of the two DRS-patient and DRS-caregiver scales were examined. A sample of 132 adults with hypertension and their family caregivers were recruited to test the psychometric properties of the DRS-C scales.

Results
The DRS-C scales indicated very satisfactory face validity with 10 family dyads in hypertension care, content validity rated by five experts (Item CVI = 0.8–1.0; Scale CVI = 0.98) and semantic equivalence rated by 22 panel members (i.e., all items were rated as 3 = relevant or 4 = very relevant by > 18 members). A well-fitting model of DRS-C-patient was identified with χ2/df = 1.47, p = 0.04, RMSEA = 0.06, GFI = 0.941, CFI = 0.961, TLI = 0.947, and SRMR = 0.019. A well-fitting model of DRS-C-caregiver was identified with χ2/df = 1.340, p = 0.092, RMSEA = 0.039, GFI = 0.940, CFI = 0.975, TLI = 0.965, and SRMR = 0.014. The Chinese DRS-patient and DRS-caregiver had satisfactory internal consistency with Cronbach’s α coefficients of 0.82 and 0.83, respectively, and test–retest reliabilities with intra-correlation coefficients of 0.97 and 0.96, respectively. The convergent validities of the Chinese versions of the DRS-patient and DRS-caregiver were very satisfactory with the self-efficacy subscale of Hypertension Self-Care Profile, and Zarit Burden Interview, respectively (Pearson’s r = − 0.70 and 0.79; both p < 0.001). Significant differences on mean scores of the Chinese versions of the DRS-patient (t = − 8.10, p < 0.001) and the DRS-caregiver (t = − 9.15, p < 0.001), between the groups of adults with hypertension and normal blood pressure counterparts.

Conclusion
Both Chinese versions of the DRS-patient and DRS-caregiver have sound psychometric properties and similar factor structure to the original English version. The Chinese versions can be valid measures of family dyadic relationship among Chinese adults with hypertension.
New aesthetic in-house 3D-printed brackets: proof of concept and fundamental mechanical properties
Authors: Papageorgiou Spyridon N. , Polychronis Georgios , Panayi Nearchos , Zinelis Spiros , Eliades Theodore 
Published: February 2022

Abstract
Objectives
Three-dimensional (3D) printing technology is an emerging manufacturing process for many orthodontic appliances, and the aim of this study was to evaluate the mechanical properties of resin-based materials as alternatives for the in-house preparation of orthodontic brackets.

Material and Methods
Two types of 3D printed resins used for temporary (T) and permanent (P) crown fabrication were included in this study. Ten blocks from each resin were manufactured by a 3D printer and, after embedding them in acrylic resin, the samples were subjected to metallographic grinding and polishing, followed by instrumented indentation testing (IIT). Martens hardness (HM), indentation modulus (EIT), and elastic index (ηIT) were determined with a Vickers indenter recording force-indentation depth curves from each specimen. After calculating descriptive statistics, differences between material types were investigated with Wilcoxon rank sum test accounting for clustering of measurements within specimens at alpha = 5%.

Results
No statistically significant differences in the mechanical properties of the two tested materials were seen: HM: median 279 N/mm2 (interquartile range [IQR] 275–287 N/mm2) for T and median 279 N/mm2 (IQR 270–285 N/mm2) for P (P value = 0.63); EIT: median 5548 MPa (IQR 5425–5834 MPa) for T and median 5644 (IQR 5420–5850 MPa) for P (P value = 0.84); ηIT: median 47.1% (46.0–47.7%) for T and median 46.0% (IQR 45.4–47.8%) for P (P value = 0.24).

Conclusions
Under the limitations of this study, it may be concluded that the mechanical properties of the two 3D printed resins tested are equal, and thus, no differences in their clinical performance are expected.
Boosting capacitive performance of manganese oxide nanorods by decorating with three-dimensional crushed graphene
Authors: Reaz Akter Hossain , Saha Shimul , Roy Chanchal Kumar , Wahab Md Abdul , Will Geoffrey , Amin Mohammed A. , Yamauchi Yusuke , Liu Shude , Kaneti Yusuf Valentino , Hossain Md. Shahriar , Firoz Shakhawat H. 
Published: February 2022

AbstractThis work reports the rational design of MnOx nanorods on 3D crushed reduced graphene oxide (MnOx/C-rGO) by chemical reduction of Ni-incorporated graphene oxide (GO) followed by chemical etching to remove Ni. The resulting MnOx/C-rGO composite synergistically integrates the electronic properties and geometry structure of MnOx and 3D C-rGO. As a result, MnOx/C-rGO shows a significantly higher specific capacitance (Csp) of 863 F g−1 than MnOx/2D graphene sheets (MnOx/S-rGO) (373 F g−1) and MnOx (200 F g−1) at a current density of 0.2 A g−1. Furthermore, when assembled into symmetric supercapacitors, the MnOx/C-rGO-based device delivers a higher Csp (288 F g−1) than MnOx/S-rGO-based device (75 F g−1) at a current density of 0.3 A g−1. The superior capacitive performance of the MnOx/C-rGO-based symmetric device is attributed to the enlarged accessible surface, reduced lamellar stacking of graphene, and improved ionic transport provided by the 3D architecture of MnOx/C-rGO. In addition, the MnOx/C-rGO-based device exhibits an energy density of 23 Wh kg−1 at a power density of 113 Wkg−1, and long-term cycling stability, demonstrating its promising potential for practical application.
Graphical Abstract
Sepsis with liver dysfunction and coagulopathy predicts an inflammatory pattern of macrophage activation
Authors: Anderko Renee R. , Gómez Hernando , Canna Scott W. , Shakoory Bita , Angus Derek C. , Yealy Donald M. , Huang David T. , Kellum John A. , Carcillo Joseph A. ,   For the ProCESS Investigators, Angus Derek C. , Barnato Amber E. , Eaton Tammy L. , Gimbel Elizabeth , Huang David T. , Keener Christopher , Kellum John A. , Landis Kyle , Pike Francis , Stapleton Diana K. , Weissfeld Lisa A. , Willochell Michael , Wofford Kourtney A. , Yealy Donald M. , Kulstad Erik , Watts Hannah , Venkat Arvind , Hou Peter C. , Massaro Anthony , Parmar Siddharth , Limkakeng Alexander T. , Brewer Kori , Delbridge Theodore R. , Mainhart Allison , Chawla Lakhmir S. , Miner James R. , Allen Todd L. , Grissom Colin K. , Swadron Stuart , Conrad Steven A. , Carlson Richard , LoVecchio Frank , Bajwa Ednan K. , Filbin Michael R. , Parry Blair A. , Ellender Timothy J. , Sama Andrew E. , Fine Jonathan , Nafeei Soheil , Terndrup Thomas , Wojnar Margaret , Pearl Ronald G. , Wilber Scott T. , Sinert Richard , Orban David J. , Wilson Jason W. , Ufberg Jacob W. , Albertson Timothy , Panacek Edward A. , Parekh Sohan , Gunn Scott R. , Rittenberger Jon S. , Wadas Richard J. , yEdwards Andrew R. , Kelly Matthew , Wang Henry E. , Holmes Talmage M. , McCurdy Michael T. , Weinert Craig , Harris Estelle S. , Self Wesley H. , Phillips Carolyn A. , Migues Ronald M. 
Published: February 2022

AbstractBackgroundInterleukin-1 receptor antagonists can reduce mortality in septic shock patients with hepatobiliary dysfunction and disseminated intravascular coagulation (HBD + DIC), an organ failure pattern with inflammatory features consistent with macrophage activation. Identification of clinical phenotypes in sepsis may allow for improved care. We aim to describe the occurrence of HBD + DIC in a contemporary cohort of patients with sepsis and determine the association of this phenotype with known macrophage activation syndrome (MAS) biomarkers and mortality. We performed a retrospective nested case–control study in adult septic shock patients with concurrent HBD + DIC and an equal number of age-matched controls, with comparative analyses of all-cause mortality and circulating biomarkers between the groups. Multiple logistic regression explored the effect of HBD + DIC on mortality and the discriminatory power of the measured biomarkers for HBD + DIC and mortality.ResultsSix percent of septic shock patients (n = 82/1341) had HBD + DIC, which was an independent risk factor for 90-day mortality (OR = 3.1, 95% CI 1.4–7.5,p = 0.008). Relative to sepsis controls, the HBD + DIC cohort had increased levels of 21 of the 26 biomarkers related to macrophage activation (p < 0.05). This panel was predictive of both HBD + DIC (sensitivity = 82%, specificity = 84%) and mortality (sensitivity = 92%, specificity = 90%).ConclusionThe HBD + DIC phenotype identified patients with high mortality and a molecular signature resembling that of MAS. These observations suggest trials of MAS-directed therapies are warranted.
Spontaneous rupture of hemorrhagic hepatic cyst: two case reports
Authors: Chogahara Ichiya , Oshita Akihiko , Nakahara Hideki , Itamoto Toshiyuki 
Published: February 2022

Abstract
Background
Spontaneous rupture of a hemorrhagic hepatic cyst is extremely rare. There is no standard treatment recommended for this condition. We report two cases of hemorrhagic hepatic cysts that spontaneously ruptured and were successfully treated with laparoscopic deroofing. We review the literature and discuss the characteristic features of spontaneous rupture of hemorrhagic hepatic cysts and their treatment.

Case presentation
The first patient was an 85-year-old man admitted for sudden-onset right hypochondralgia and fever. Computed tomography revealed a 13-cm hepatic cyst occupying the right lobe of the liver and spontaneous rupture of the cyst. Laparoscopic deroofing was performed and continuous oozing from the cystic wall was found. Histological examination revealed a simple hepatic cyst. The patient was discharged on postoperative day 6. In the second case, a 77-year-old woman who had been followed up for a simple hepatic cyst (13 cm) was admitted for sudden onset of right hypochondralgia. Computed tomography demonstrated a 9.9-cm hepatic cyst occupying segment 4 of the liver. Laparoscopic deroofing was performed and continuous oozing from the cystic wall was observed. Histological examination revealed a simple hepatic cyst. The patient was discharged on postoperative day 6.

Conclusion
Laparoscopic deroofing was performed in patients with spontaneous rupture of hemorrhagic nonparasitic hepatic cysts.
Massive hemoptysis managed by prolonged anticoagulation-free veno-venous extracorporeal membrane oxygenation with which thoracic surgeries were accompanied: a case report
Authors: Kimura Tomonori , Kobayashi Tomoe , Kobori Fumimasa , Goto Maria , Takemitsu Mikako , Tanaka Atsuko , Sato Jiro , Isomine Shinji 
Published: February 2022

Abstract
Background
Massive hemoptysis causing inadequate ventilation results in life-threatening consequences. We present a patient who developed respiratory insufficiency produced by bronchiectatic massive hemoptysis and underwent prolonged anticoagulation-free veno-venous extracorporeal membrane oxygenation (VV-ECMO) during which thoracic surgeries were performed.

Case presentation
A 79-year-old woman suffered massive hemoptysis resulting in respiratory failure during fiberoptic bronchoscopy. Bronchial intubation followed by one lung ventilation failed to ensure adequate oxygenation. Anticoagulation-free VV-ECMO, therefore, was installed immediately. Since conservative hemostatic measures including bronchial arterial embolization were not effective, resection of the culprit lung was performed while on VV-ECMO. Next day an exploratory thoracotomy and intercostal artery embolization were needed for recurrent bleeding. The VV-ECMO was withdrawn after five days of operation.

Conclusions
Massive hemoptysis can be fatal and needs instantaneous and intensive treatments. In our case, long-term anticoagulation-free VV-ECMO during which thoracic surgeries and endovascular interventions were performed provided a favorable outcome.