bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of combination therapy with Shenfu Injection in critically ill patients with septic shock receiving mechanical ventilation: A multicentric, real-world study
Authors: Li Xiaoqian , Huang Fan , Zhu Lixia , Luo Tianyi , Zhang Yuzhuo , Gu Huiwen , Guo Liheng , Mao Shuai 
Published: November 2022

Background:Septic shock has increasingly become a cause of death threatening human survival. Shenfu Injection (SFI), a patented Chinese medicine, has been widely used in the treatment of patients with sepsis and cardiovascular diseases domestically. We sought to examine whether combination therapy with SFI can improve clinical outcomes in critically ill patients undergoing mechanical ventilation (MV).Methods:This real-world, multicenter retrospective trial enrolled consecutive adult patients with sepsis requiring MV from four medical/surgical intensive care units (ICUs) in China between August 2016 and September 2021. Patients were identified from the medical information department database of each center and assigned to either of two groups (SFI or control) on the basis of the initial treatment received. The primary outcome was 28-day all-cause mortality, and the durations of vasopressor therapy and MV, the ICU length of stay, and costs were assessed as secondary outcomes. Subsequently, we performed a meta-analysis of randomized controlled trials (RCTs) on SFI published before July 2021 to verify our conclusions.Results:2311 mechanically ventilated patients with septic shock (1128 patients in the SFI group and 1183 in the control group) were analyzed. The survival probability during the first 28 days after admission in the SFI group was greater than that in the control group [p&lt; 0.01 by log-rank test; hazard ratio (HR), 0.56; 95% confidence interval (CI), 0.39–0.72]. Patients in the SFI group also experienced a significantly reduced duration of vasopressor therapy [7.28 (95% CI, 6.14–8.42)vs.12.06 (95% CI, 10.71–13.41) days,p&lt; 0.001], more ventilator-free days [6.49 (95% CI, 5.42–7.55)vs.10.84 (95% CI, 9.59–12.09) days,p&lt; 0.001], a shorter ICU length of stay [18.48 (95% CI, 17.59–19.38)vs.23.77 (95% CI, 22.47–25.07) days,p&lt; 0.001], and more time free from organ failure [14.23 (95% CI, 12.94–15.52)vs.19.07 (95% CI, 16.09–22.05) days,p&lt; 0.001]. No major adverse effects were reported in either group.Conclusion:Among critically ill patients requiring MV, combination therapy with SFI can improve the survival probability without any obvious adverse reactions.
SLC7A8 coding for LAT2 is associated with early disease progression in osteosarcoma and transports doxorubicin
Authors: Hurkmans Evelien G. E. , Koenderink Jan B. , van den Heuvel Jeroen J. M. W. , Versleijen-Jonkers Yvonne M. H. , Hillebrandt-Roeffen Melissa H. S. , Groothuismink Johanne M. , Vos Hanneke I. , van der Graaf Winette T. A. , Flucke Uta , Muradjan Grigor , Schreuder Hendrik W. B. , Hagleitner Melanie M. , Brunner Han G. , Gelderblom Hans , Cleton-Jansen Anne-Marie , Guchelaar Henk-Jan , de Bont Eveline S. J. M. , Touw Daan J. , Nijhoff G. Jan , Kremer Leontien C. M. , Caron Huib , Windsor Rachael , Patiño-García Ana , González-Neira Anna , Saletta Federica , McCowage Geoff , Nagabushan Sumanth , Catchpoole Daniel , te Loo D. Maroeska W. M. , Coenen Marieke J. H. 
Published: November 2022

Background: Despite (neo) adjuvant chemotherapy with cisplatin, doxorubicin and methotrexate, some patients with primary osteosarcoma progress during first-line systemic treatment and have a poor prognosis. In this study, we investigated whether patients with early disease progression (EDP), are characterized by a distinctive pharmacogenetic profile.Methods and Findings: Germline DNA from 287 Dutch high-grade osteosarcoma patients was genotyped using the DMET Plus array (containing 1,936 genetic markers in 231 drug metabolism and transporter genes). Associations between genetic variants and EDP were assessed using logistic regression models and associated variants (p &lt;0.05) were validated in independent cohorts of 146 (Spain and United Kingdom) and 28 patients (Australia). In the association analyses, EDP was significantly associated with an SLC7A8 locus and was independently validated (meta-analysis validation cohorts: OR 0.19 [0.06–0.55], p = 0.002). The functional relevance of the top hits was explored by immunohistochemistry staining and an in vitro transport models. SLC7A8 encodes for the L-type amino acid transporter 2 (LAT2). Transport assays in HEK293 cells overexpressing LAT2 showed that doxorubicin, but not cisplatin and methotrexate, is a substrate for LAT2 (p &lt; 0.0001). Finally, SLC7A8 mRNA expression analysis and LAT2 immunohistochemistry of osteosarcoma tissue showed that the lack of LAT2 expression is a prognostic factor of poor prognosis and reduced overall survival in patients without metastases (p = 0.0099 and p = 0.14, resp.).Conclusion: This study identified a novel locus in SLC7A8 to be associated with EDP in osteosarcoma. Functional studies indicate LAT2-mediates uptake of doxorubicin, which could give new opportunities to personalize treatment of osteosarcoma patients.
Effects of Sophora flavescens aiton and the absorbed bioactive metabolite matrine individually and in combination with 5-fluorouracil on proliferation and apoptosis of gastric cancer cells in nude mice
Authors: Hu Huan-Fu , Wang Zheng , Tang Wen-Li , Fu Xue-Ming , Kong Xiang-Jun , Qiu Ying-Kun , Xi Sheng-Yan 
Published: November 2022

Background:Sophora flavescens aiton (SFA) and its main bioactive metabolite matrine are widely used in traditional Chinese medicine (TCM) preparations and have achieved good curative effects for the treatment of various tumors. However, the mechanisms underlying SFA and matrine individually and in combination with chemotherapeutic drugs for treatment of gastric cancer (GC) remain unclear.Aim of the study: To elucidate the mechanisms underlying the ability of SFA and matrine individually and in combination with chemotherapeutic drugs to inhibit proliferation and promote apoptosis of human GC cells.Materials and methods: Forty-eight nude mice were randomly divided into six groups that were treated with normal saline (model group), 5-fluorouracil (5-FU), SFA decoction (SFAD), matrine, SFAD+5-FU, or matrine+5-FU. A subcutaneous heterotopic tumor model was established in nude mice by implantation of human GC BGC-823 cells. All mice were treated for 28 days. Bioactive metabolites in SFA were determined by HPLC-MS/MS. The tumor volume, tumor weight, and tumor inhibition rate of mice were documented. Histopathology and ultramicroscopic pathology of tumor tissues were observed. The tumor cell cycle and apoptosis in vivo were detected. Serum levels of PCNA, BAX, Bcl-2, Caspase-9, Caspase-3 and cleaved Caspase-3 were measured. Protein levels of MS4A10, MS4A8, MS4A7, PCNA, BAX, Bcl-2, Caspase-3, and cleaved Caspase-3 were measured in tumor tissues.Results: Both SFAD and matrine inhibited the growth of transplanted GC cells, which was more effective when combined with 5-FU. The tumor inhibition rates of the 5-FU, SFAD, matrine, SFAD+5-FU, and matrine+5-FU groups were 53.85%, 33.96%, 30.44%, 59.74%, and 56.55%, respectively. The body weight of tumor-bearing nude mice was greater in the SFAD group than the normal saline and matrine groups. SFAD+5-FU and matrine+5-FU blocked BGC-823 cells in the G0-G1/S transition, promoted apoptosis, and significantly decreased the content of serum apoptosis-inhibitory proteins (PCNA and Bcl-2) as well as protein expression of MS4A8, MS4A10, Bcl-2, and PCNA in tumor tissues, while increasing serum levels of pro-apoptotic proteins (Caspase-9, Caspase-3 and cleaved-Caspase-3) and protein expression of BAX and cleaved-Caspase-3 in tumor tissues.Conclusion: SFAD and matrine both individually and in combination with 5-FU ameliorated malignancy of transplanted tumors by reducing proliferation and promoting apoptosis of BGC-823 cells. These findings confirm the anti-tumor synergistic effect of TCM and chemotherapeutic drugs.
State-to-state dynamics of the C+(2P) + SH(X2Π) → H(2S) + CS+(X2Σ+) reaction using a time-dependent wave packet and quasi-classical trajectory methods
Authors: Liu Dong , Zhao Juan , Wang Wei , Song Yuzhi , Meng Qingtian , Zhang Lulu 
Published: November 2022

The time-dependent quantum wave packet (TDWP) and quasi-classical trajectory (QCT) are the basic research methods of reaction dynamics. Utilizing these two methods, the total reaction probability (J = 0), integral cross section (ICS), and rate parameter for the C+(2P) + SH(X2Π)(v = 0, 1, 2, 3) → H(2S) + CS+(X2Σ+) reactions are calculated on an accurate potential energy surface [Zhang et al. Phys. Chem. Chem. Phys. 2022, 24, 1007]. The results of QCT are slightly different from those of the TDWP in value, but the trend is consistent. They are also weakly dependent on the initial vibrational excitation of SH. The state-to-state reaction probability and ICS at fixed collision energies (0.1, 0.3, 0.5, and 0.7 eV) are first calculated using QCT methods. It is hoped that our work can attract experimentalists to study the dynamics of this interesting but rarely discussed system.
Real-time 3D echocardiographic transilluminated imaging combined with artificially intelligent left atrial appendage measurement for atrial fibrillation interventional procedures
Authors: Sun Aijiao , Ren Sihua , Xiao Yangjie , Chen Yixin , Wang Nan , Li Chendi , Tan Xueying , Pan Yilong , Sun Feifei , Ren Weidong 
Published: November 2022

Aims: This study investigated the feasibility and accuracy of real-time three-dimensional (3D) echocardiographic transilluminated imaging (TrueVue Glass) in left atrial appendage (LAA) anatomical morphology and artificial intelligence (AI)-assisted 3D automated LAA measurement (3D Auto LAA) software in the preoperative evaluation of LAA occlusion (LAAO) in patients with atrial fibrillation (AF).Method and results: Thirty-seven patients with AF were selected. Two-dimensional (2D) and real-time 3D transesophageal echocardiography (RT3D-TEE) were performed preoperatively, using conventional 3D, the new 3D TrueVue Glass mode, and cardiac computed tomography angiography (CCTA) to assess and type the morphology of LAA. Physiological parameters were measured using traditional 2D and 3D manual (3D Manual LAA), 3D Auto LAA, and CCTA. TrueVue Glass for LAA outer contour display was compared with CCTA. Comparisons were based on correlation and consistency in measuring the maximum diameter (LZ max), minimum diameter (LZ min), area (LZ area), and circumference (LZ cir) of LAA landing zone (LZ). Times and variabilities were compared. The concordance rate for external shape of LAA was 97.14% between TrueVue Glass and CCTA. 3D Auto LAA and 3D Manual LAA have a stronger correlation and higher consistency in all parameters. 3D Auto LAA showed higher intra- and interobserver reproducibility and allowed quicker analysis (p &lt; 0.05). LAAO was performed in 35 patients (94.59%), and none of which had serious adverse events.Conclusion: TrueVue Glass is the first non-invasive and radiation-free visualization of the overall external contour of LAA and its adjacent structures. 3D Auto LAA simplifies the measurement, making the preoperative assessment more efficient and convenient while ensuring the accuracy and reproducibility. A combination of the two is feasible for accurate and rapid assessment of LAA anatomy and physiology in AF patients and has practical application in LAAO.
Field application of silicon alleviates drought stress and improves water use efficiency in wheat
Authors: Johnson Scott N. , Chen Zhong-Hua , Rowe Rhiannon C. , Tissue David T. 
Published: November 2022

Detrimental impacts of drought on crop yield have tripled in the last 50 years with climate models predicting that the frequency of such droughts will intensify in the future. Silicon (Si) accumulation, especially in Poaceae crops such as wheat (Triticum aestivum L.), may alleviate the adverse impacts of drought. We have very limited information, however, about whether Si supplementation could alleviate the impacts of drought under field conditions and no studies have specifically manipulated rainfall. Using field–based rain exclusion shelters, we determined whether Si supplementation (equivalent to 39, 78 and 117 kg ha-1) affected T. aestivum growth, elemental chemistry [Si, carbon (C) and nitrogen (N)], physiology (rates of photosynthesis, transpiration, stomatal conductance, and water use efficiency) and yield (grain production) under ambient and drought (50% of ambient) rainfall scenarios. Averaged across Si treatments, drought reduced shoot mass by 21% and grain production by 18%. Si supplementation increased shoot mass by up to 43% and 73% in ambient and drought water treatments, respectively, and restored grain production in droughted plants to levels comparable with plants supplied with ambient rainfall. Si supplementation increased leaf-level water use efficiency by 32–74%, depending on Si supplementation rates. Water supply and Si supplementation did not alter concentrations of C and N, but Si supplementation increased shoot C content by 39% and 83% under ambient and drought conditions, respectively. This equates to an increase from 6.4 to 8.9 tonnes C ha-1 and from 4.03 to 7.35 tonnes C ha-1 under ambient and drought conditions, respectively. We conclude that Si supplementation ameliorated the negative impacts of drought on T. aestivum growth and grain yield, potentially through its beneficial impacts on water use efficiency. Moreover, the beneficial impacts of Si on plant growth and C storage may render Si supplementation a useful tool for both drought mitigation and C sequestration.
We can’t afford the candy! The influence of parenting by lying about money on mobile phone addiction and the roles of anxiety and socioeconomic status among Chinese adolescents
Authors: Wei Hua , Lu Lijun , Liu Meiting 
Published: November 2022

BackgroundExtant research has indicated that parenting practice, such as harsh parenting, rejection, and neglect increases the risk of mobile phone addiction. However, no research to date has examined the association between parenting by lying about money and adolescent mobile phone addiction.ObjectiveThe current study used a survey to test whether parenting by lying about money may be associated with adolescent mobile phone addiction. The mediation of anxiety and moderation of socioeconomic status were also examined.Materials and methodsWe recruited 971 adolescents from five secondary schools in a city in central China. Of the participants, 448 (46.14%) were boys and 523 (53.86%) were girls (Mage = 13.63, SDage = 1.01).ResultsThe results of mediation analysis indicated that parenting by lying about money positively predicted mobile phone addiction (B = 0.144, p &lt; 0.01); parenting by lying about money positively predicted anxiety (B = 0.126, p &lt; 0.01) and mobile phone addiction (B = 0.107, p &lt; 0.01). Anxiety positively predicted mobile phone addiction (B = 0.293, p &lt; 0.01). Moreover, the bias-corrected bootstrapping mediation test indicated that the process by which parenting by lying about money predicted mobile phone addiction through anxiety was significant (indirect effect = 0.037, SE = 0.011, 95% CI = [0.017, 0.059]).ConclusionThe current study suggests that parenting by lying about money may lead to mobile phone addiction through the mediation of anxiety. However, the effect was stronger for adolescents with higher level of socioeconomic status than their counterparts.
Association between screen time and hyperactive behaviors in children under 3 years in China
Authors: Wu Jian-Bo , Yin Xiao-Na , Qiu Shuang-Yan , Wen Guo-Ming , Yang Wei-Kang , Zhang Jing-Yu , Zhao Ya-Fen , Wang Xin , Hong Xiao-Bing , Lu DaLi , Jing Jin 
Published: November 2022

BackgroundScreen time during early life has increased dramatically among Chinese children. Excessive screen time has raised growing concerns about the neuropsychological development of children. The effects of screen exposure on early life and the boundary between screen time and hyperactive behaviors are well worth investigating. We examined associations between screen time and hyperactive behaviors in children under the age of 3 years using data from the Longhua Children Cohort Study (LCCS).MethodsA cross-sectional study was conducted among 42,841 3-year-old children from Longhua District, Shenzhen. Information on socio-demographic characteristics, children’s annual screen time since birth, and hyperactive behaviors (measured by the Conners Parental Symptom Questionnaire) was collected through self-administered structured questionnaires completed by the primary caregiver. A series of logistic regression models assessed the association between screen time and hyperactive behaviors.ResultsThe average daily screen time of children under the age of 3 years was 55.83 ± 58.54 min, and screen time increased with age. Binomial logistic regression analysis found that the earlier the screen exposure, the greater the risk of hyperactive behaviors. Using binary logistic regression model, after controlling for confounding factors, the study found that more screen time was more associated with hyperactive behaviors. For children aged 0–3 years with daily screen time exceeding 90, 120, 150, and 180 min, the risk values for hyperactive behaviors were 1.98 [95% confidence interval (CI): 1.05, 3.78), 2.71 (95%CI:1.38, 5.30), 3.17 (95% CI: 1.50, 6.65), and 4.62 (95% CI: 2.45, 8.71)], respectively.ConclusionEarly screen exposure may be associated with hyperactive behaviors in children under the age of 3 years. More than 90 min of screen time per day in children under 3 years was associated with hyperactive behaviors. The findings support the importance of screen time interventions for children under 3 years.
On the impacts of the COVID-19 pandemic on mortality: Lost years or lost days?
Authors: Rousson Valentin , Locatelli Isabella 
Published: November 2022

ObjectiveTo quantify the (direct and indirect) impacts of the COVID-19 pandemic on mortality for actual populations of persons living in 12 European countries in 2020.MethodBased on demographic and mortality data, as well as remaining life expectancies found in the Human Mortality Database, we calculated a “population life loss” in 2020 for men and women living in Belgium, Croatia, Denmark, Finland, Hungary, Lithuania, Luxembourg, Norway, Portugal, Spain, Sweden, and Switzerland. This quantity was obtained by dividing the total number of years lost in 2020 (estimated from all-cause mortality data and attributed directly or indirectly to COVID-19) by the size of the population.ResultsA significant population life loss was found in 8 countries in 2020, with men losing an average of 8.7, 5.0, 4.4, 4.0, 3.7, 3.4, 3.1, and 2.7 days in Lithuania, Spain, Belgium, Hungary, Croatia, Portugal, Switzerland, and Sweden, respectively. For women, this loss was 5.5, 4.3, 3.7, 3.7, 3.1, 2.4, 1.6, and 1.4 days, respectively. No significant losses were found in Finland, Luxembourg, Denmark and Norway. Life loss was highly dependent on age, reaching 40 days at the age of 90 in some countries, while only a few significant losses occurred under the age of 60. Even in countries with a significant population life loss in 2020, it was on average about 30 times lower than in 1918, at the time of the Spanish flu.ConclusionsOur results based on the concept of population life loss were consistent with those based on the classical concept of life expectancy, confirming the significant impact of COVID-19 on mortality in 8 European countries in 2020. However, while life expectancy losses were typically counted in months or years, population life losses could be counted in days, a potentially useful piece of information from a public health perspective.
The grit crust: A poly-extremotolerant microbial community from the Atacama Desert as a model for astrobiology
Authors: Jung Patrick , Lehnert Lukas W. , Bendix Jörg , Lentendu Guillaume , Grube Martin , Alfaro Fernando D. , Rio Camilo del , Gutiérrez Alvarado José Luis , van den Brink Liesbeth , Lakatos Michael 
Published: November 2022

The grit crust is a recently discovered, novel type of biocrust made of prokaryotic cyanobacteria, eukaryotic green algae, fungi, lichens and other microbes that grow around and within granitoid stone pebbles of about 6 mm diameter in the Coastal Range of the Atacama Desert, Chile. The microbial community is very well adapted towards the extreme conditions of the Atacama Desert, such as the highest irradiation of the planet, strong temperature amplitudes and steep wet-dry cycles. It also has several other striking features making this biocrust unique compared to biocrusts known from other arid biomes on Earth. It has already been shown that the grit crust mediates various bio-weathering activities in its natural habitat. These activities prime soil for higher organisms in a way that can be envisioned as a proxy for general processes shaping even extra-terrestrial landscapes. This mini-review highlights the potential of the grit crust as a model for astrobiology in terms of extra-terrestrial microbial colonization and biotechnological applications that support human colonization of planets.
One hundred most cited articles related to Endoscopic retrograde cholangiopancreatography: A bibliometric analysis
Authors: Xu Xuan , Guan Lulu , Wu Yao , Ke Huajing , Zhao Yuanbin , Liu Pi 
Published: November 2022

BackgroundEndoscopic retrograde cholangiopancreatography (ERCP) has developed over the past few decades into a reliable technology for diagnostic and therapeutic purposes. Through a bibliometric analysis, this research attempted to evaluate the characteristics of the top 100 articles on ERCP that had the most citations.MethodsWe extracted pertinent publications from the Web of Science Core Collection (WoSCC) on July 9, 2022. The top 100 ERCP articles with the most citations were identified and analyzed. The following data were extracted: publication year, country/region, organization, total citation times, annual citation times, research type and research field, etc. To implement the network’s visual analysis, a bibliographic coupling network based on keywords was built using the VOSviewer 1.6.17 program.ResultsThe journal with the most publications were GASTROINTESTINAL ENDOSCOPY, with 45 articles. Most of the top 100 articles came from the United States (n = 47) and Italy (n = 14). Indiana University and the University of Amsterdam were among the most important institutions in ERCP research. ML Freeman of the University of Minnesota contributed the highest number (n = 9) and the most highly cited paper. The age of the paper and article type is closely related to citation frequency. Of the 100 most-cited articles, clinical application in the field of ERCP has focused on three aspects: diagnosis, treatment, and complications. Clinical use of ERCP has shifted from diagnosis to treatment. Post-ERCP pancreatitis is the focus of attention, and the clinical application of technically complex therapeutic ERCP is the future development trend.ConclusionThis study lists the most influential articles in ERCP by exposing the current state of the field, and showing the evolution of research trends to provide perspective for the future development of ERCP.