bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early Change in Area Strain Detected by 3D Speckle Tracking Is Associated With Subsequent Cardiotoxicity in Patients Treated With Low Doses of Anthracyclines
Authors: Piveta Rafael B. , Rodrigues Ana Clara T. , Vieira Marcelo L. C. , Fischer Cláudio H. , Afonso Tania R. , Daminello Edgar , Cruz Felipe M. , Galvão Tatiana F. G. , Filho Edgar B. L. , Katz Marcelo , Morhy Samira S. 
Published: March 2022

ObjectiveTo evaluate the prognostic impact of the parameters of myocardial deformation using three-dimensional speckle tracking echocardiography (3DSTE) in patients with breast cancer who underwent chemotherapy with low doses of anthracyclines.BackgroundChemotherapy-related cardiotoxicity has an important prognostic impact on cancer survivors. Three-dimensional STE has revealed more consistent data than two-dimensional techniques and may represent a more accurate tool in the evaluation of myocardial function in patients who underwent chemotherapy.MethodsWe evaluated patients with breast cancer who were treated with anthracyclines (associated or not with trastuzumab) in five stages: baseline, after cumulative doses of 120 and 240 mg/m2 of doxorubicin, and then, after 6 months and at least 1 year after anthracyclines. Ultrasensitive troponin I (US-TnI) and a standard echocardiography study were performed at each stage. We analyzed left ventricular ejection fraction (LVEF) by Simpson's method, two-dimensional speckle tracking (2DSTE) with longitudinal and radial strain values, and 3DSTE with longitudinal, radial, and circumferential strain as well as twist, torsion, rotation, and three-dimensional global area strain (3DGAS). Cardiotoxicity was defined as a decrease in LVEF by more than 10 percentage points to a value lower than 53%.ResultsWe evaluated 51 female patients who were aged 50.6 ± 11 years. After the cumulative dose of 240 mg/m2 of doxorubicin, US-TnI was increased (&gt;34 pg/ml) in 21 patients (45%, p &gt; 0.001), LVEF remained unchanged (p = 0.178), while 2DSTE longitudinal strain was decreased (from −17.8% to −17.1%, p &lt; 0.001) and 3DSTE detected changes in longitudinal, radial, circumferential, and area strain. After a lower cumulative dose of doxorubicin (120 mg/m2), 3DGAS (p &lt; 0.001) was the only parameter that was changed. In the follow-up, 7 (13%) patients presented a decrease in LVEF. Three-dimensional GAS early changed to abnormal values was the only variable associated with a subsequent decrease in LVEF (definitive cardiotoxicity).ConclusionIn patients with breast cancer, 3DSTE detected early changes in area strain after very low doses of doxorubicin. The 3DGAS early changed to abnormal values was associated with a subsequent decrease in LVEF, representing a promising technique to predict chemotherapy-induced cardiomyopathy.
Energy Drinks: Effects on Blood Pressure and Heart Rate in Children and Teenagers. A Randomized Trial
Authors: Oberhoffer Felix Sebastian , Li Pengzhu , Jakob André , Dalla-Pozza Robert , Haas Nikolaus Alexander , Mandilaras Guido 
Published: March 2022

BackgroundThe consumption of Energy Drinks (ED) is very popular among children and teenagers. While potential cardiovascular side effects of these beverages are suggested, the acute impact of ED consumption on the pediatric cardiovascular system has not been systematically examined yet. The aim of this study was to investigate the acute effects of ED consumption on blood pressure and heart rate in healthy children and teenagers.MethodsThis study was a randomized, single-blind, placebo-controlled, crossover clinical trial. On two consecutive days, the study participants were asked to consume a weight-adjusted amount of an ED (3 mg caffeine per kg of body weight) or a placebo containing a similar amount of sugar but without conventional ED ingredients. Systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate were measured at the following time points: baseline as well as 30, 60, 120 and 240 min after beverage consumption.ResultsIn total, 27 healthy children and adolescents (mean age 14.53 ± 2.40 years, 14 male) were included in the present study. Compared to placebo intake, mean SBP was demonstrated to be up to 5.23 mmHg (p &lt; 0.0001) and mean DBP up to 3.29 mmHg (p &lt; 0.001) increased after ED consumption. Prevalence of elevated blood pressure, stage 1 and stage 2 hypertension was higher after ED consumption. Heart rate tended to be lower after ED consumption.ConclusionsThe acute ED consumption is associated with a significantly increased SBP and DBP in healthy children and teenagers. Minors, particularly those with pre-existing health conditions, should be discouraged from drinking EDs.Clinical Trial Registrationhttps://www.drks.de/drks_web/, identifier: DRKS00027580.
The Dynamic of PRAMEY Isoforms in Testis and Epididymis Suggests Their Involvement in Spermatozoa Maturation
Authors: Kern Chandlar H. , Feitosa Weber B. , Liu Wan-Sheng 
Published: March 2022

The preferentially expressed antigen in melanoma, Y-linked (PRAMEY) is a cancer/testis antigen expressed predominantly in bovine spermatogenic cells, playing an important role in germ cell formation. To better understand PRAMEY’s function during spermatogenesis, we studied the dynamics of PRAMEY isoforms by Western blotting (WB) with PRAMEY-specific antibodies. The PRAMEY protein was assessed in the bovine testicular and epididymal spermatozoa, fluid and tissues, and as well as in ejaculated semen. The protein was further examined, at a subcellular level in sperm head and tail, as well as in the subcellular components, including the cytosol, nucleus, membrane, and mitochondria. RNA expression of PRAMEY was also evaluated in testis and epididymal tissues. Our WB results confirmed the previously reported four isoforms of PRAMEY (58, 30, 26, and 13 kDa) in the bovine testis and spermatozoa. We found that testicular spermatozoa expressed the 58 and 30 kDa isoforms. As spermatozoa migrated to the epididymis, they expressed two additional isoforms, 26 and 13 kDa. Similarly, the 58 and 30 kDa isoforms were detected only in the testis fluid, while all four isoforms were detected in fluid from the cauda epididymis. Tissue evaluation indicated a significantly higher expression of the 58 and 13 kDa isoforms in the cauda tissue when compared to both the testis and caput tissue (p &lt; 0.05). These results indicated that testis samples (spermatozoa, fluid, and tissue) expressed predominantly the 58 and 30 kDa PRAMEY isoforms, suggesting their involvement in spermatogenesis. In contrast, the 26 kDa isoform was specific to epididymal sperm and the 13 kDa isoform was marked in samples derived from the cauda epididymis, suggesting their involvement in sperm maturation. Results from the sperm head and tail experiments indicated that the 13 kDa isoform increased 4-fold in sperm tails from caput to cauda, suggesting this isoform may have a significant role in tail function. Additionally, the 13 kDa isoform increased significantly (p &lt; 0.05) in the cytosol during epididymal passage and tended to increase in other subcellular components. The expression of PRAMEY in the sperm subcellular components during epididymal maturation suggests the involvement of PRAMEY, especially the 13 kDa isoform, in sperm motility.
PSRR: A Web Server for Predicting the Regulation of miRNAs Expression by Small Molecules
Authors: Yu Fanrong , Li Bihui , Sun Jianfeng , Qi Jing , De Wilde Rudy Leon , Torres-de la Roche Luz Angela , Li Cheng , Ahmad Sajjad , Shi Wenjie , Li Xiqing , Chen Zihao 
Published: March 2022

Background: MicroRNAs (miRNAs) play key roles in a variety of pathological processes by interacting with their specific target mRNAs for translation repression and may function as oncogenes (oncomiRs) or tumor suppressors (TSmiRs). Therefore, a web server that could predict the regulation relations between miRNAs and small molecules is expected to achieve implications for identifying potential therapeutic targets for anti-tumor drug development.Methods: Upon obtaining positive/known small molecule-miRNA regulation pairs from SM2miR, we generated a multitude of high-quality negative/unknown pairs by leveraging similarities between the small molecule structures. Using the pool of the positive and negative pairs, we created the Dataset1 and Dataset2 datasets specific to up-regulation and down-regulation pairs, respectively. Manifold machine learning algorithms were then employed to construct models of predicting up-regulation and down-regulation pairs on the training portion of pairs in Dataset1 and Dataset2, respectively. Prediction abilities of the resulting models were further examined by discovering potential small molecules to regulate oncogenic miRNAs identified from miRNA sequencing data of endometrial carcinoma samples.Results: The random forest algorithm outperformed four machine-learning algorithms by achieving the highest AUC values of 0.911 for the up-regulation model and 0.896 for the down-regulation model on the testing datasets. Moreover, the down-regulation and up-regulation models yielded the accuracy values of 0.91 and 0.90 on independent validation pairs, respectively. In a case study, our model showed highly-reliable results by confirming all top 10 predicted regulation pairs as experimentally validated pairs. Finally, our predicted binding affinities of oncogenic miRNAs and small molecules bore a close resemblance to the lowest binding energy profiles using molecular docking. Predictions of the final model are freely accessible through the PSRR web server at https://rnadrug.shinyapps.io/PSRR/.Conclusion: Our study provides a novel web server that could effectively predict the regulation of miRNAs expression by small molecules.
Occupational Stress and the Quality of Life of Nurses in Infectious Disease Departments in China: The Mediating Role of Psychological Resilience
Authors: Yan Jiaran , Wu Chao , Du Yanling , He Shizhe , Shang Lei , Lang Hongjuan 
Published: March 2022

AimWe aim to explore the impact of occupational stress on the quality of life of nurses in infectious disease departments and to explore the mediating role of psychological resilience on this impact.BackgroundSudden public health events and the prevalence of infectious diseases give nurses in infectious disease departments a heavy task load and high occupational stress, which can affect their quality of life, and which is closely related to the quality of clinical care they provide. There are few existing studies on occupational stress, psychological resilience, and the quality of life of nurses in infectious disease departments.MethodsWe collected data from infectious-disease-specialized hospitals or infectious disease departments of general hospitals in China. In total 1,536 nurses completed questionnaires: the Effort-Reward Imbalance Questionnaire, the Connor-Davidson Resilience Scale, and the World Health Organization Quality of Life Brief Scale. We use a structural equation model to test the mediating role of the psychological resilience in the relationship between occupational stress and quality of life.ResultsAmong 1,536 participants, 88.2% experienced an effort-reward imbalance. The average scores for psychological resilience and quality of life were 56.06 (SD = 14.19) and 51.80 (SD = 8.23), respectively. Our results show that occupational stress is negatively correlated with psychological resilience (r = −0.28, p &lt; 0.01) and quality of life (r = −0.44, p &lt; 0.01). In addition, we find that psychological resilience is positively correlated with quality of life (r = 0.55, p &lt; 0.01) and that the indirect effect of occupational stress on quality of life through psychological resilience is significant (β = −0.036, 95% CI: 0.027 to 0.426), indicating at least a partial mediating role of psychological resilience.ConclusionA high proportion of nurses in infectious disease departments felt that their jobs’ effort-reward imbalance was high. These nurses’ scores for psychological resilience were in the middle level among Chinese people generally, but their quality of life was lower than the Chinese norm. We conclude that occupational stress has an important impact on their quality of life, and psychological resilience plays a partial mediating role on this impact.Implications for Nursing ManagementHospital managers can benefit from paying attention to the occupational stress of nurses and helping to improve the quality of life of nurses by alleviating this occupational stress and improving psychological resilience.
Individual Preventive Behaviors of COVID-19 and Associated Psychological Factors Among Chinese Older Adults: A Cross-Sectional Online Survey
Authors: Duan Yanping , Hu Chun , Lin Zhihua , Liang Wei , Shang Borui , Baker Julien Steven , He Jiali , Wang Yanping 
Published: March 2022

PurposeOlder adults aged 60 years and above are classified as being of high-risk for infection during the COVID-19 pandemic. This study aimed to investigate the associations of psychological factors (motivational factors: risk perception, health knowledge, attitude, subjective norm, motivational self-efficacy, and intention; volitional factors: volitional self-efficacy, planning, and action control) of preventive behaviors with three preventive behaviors (hand washing, facemask wearing, and social distancing) among Chinese older adults during the COVID-19 pandemic.MethodsA cross-sectional questionnaire survey was administered via SOJUMP, a widely used online survey platform in China. A total of 928 older adults (mean = 67.24 years, age range: 60–90 years, SD = 6.43, 55.9% females) were recruited using a snowball sampling approach from Hubei Province (n = 667) and outside Hubei Province (n = 261) in China during May 18, 2020 to June 7, 2020. Multiple hierarchical regressions were conducted with four models to examine the association between demographic, past behavior, psychological factors and each preventive behavior.Principal FindingsAll three preventive behaviors in older adults increased dramatically during the pandemic of COVID-19. Gender, living status, educational level, past behavior, health knowledge, intention and planning significantly predicted hand washing behavior, R2 = 0.395, F(10, 927) = 54.372, p &lt; 0.001. Gender, education level, important others (e.g., family members or friends) infection, past behavior, health knowledge, planning and action control significantly predicted mask wearing behavior, R2 = 0.202, F(10, 927) = 23.197, p &lt; 0.001. Living place, past behavior and health knowledge significantly predicted social distancing behavior, R2 = 0.204, F(9, 927) = 26.201, p &lt; 0.001.Major ConclusionsPast behavior and health knowledge predicted all three preventive behaviors. Planning was an important psychological factor for both hand washing and mask wearing behaviors. All those critical demographic and psychological factors are critical for future interventions to facilitate older adults to comply with three preventive behaviors in daily life and to stay healthy during the COVID-19 pandemic.
Upper Elk Point subgroup paleogeography and evaporite distribution with implications for evaporite dissolution, karstification, and carbonate diagenesis in northeastern Alberta
Authors: Hauck Tyler E. , Grobe Matthias 
Published: March 2022

Abstract
Lithostratigraphic correlation and mapping of formations and units within the Upper Elk Point subgroup provide updated information on their extent and distribution within the province of Alberta. Together with detailed bed-scale evaporite mapping of three evaporite minerals — halite, anhydrite, and gypsum — within evaporitic successions for net-thickness maps, these data allow new representations of the paleogeography of these units across the province. Paleogeographic maps of the Keg River, Prairie Evaporite and Muskeg formations reveal new details on the location of the La Crete sub-basin in northern Alberta, and the distribution and nature of Keg River Formation buildups and the overlying evaporite strata within this depositional realm. Net-evaporite mapping gives a robust picture of the distribution of Upper Elk Point subgroup evaporites, and allows for a detailed characterization of heterogeneities, halite dissolution, and sulphate karstification. Mapping of gypsum reveals that rehydration of anhydrite to gypsum (gypsification) through meteoric inflow is most pronounced within the La Crete sub-basin in northeastern Alberta, particularly where thick anhydrite deposits are associated with interbuildup basinal areas east of the Prairie Evaporite halite dissolution scarp. This association provides an explanation for the location of where active gypsification, dissolution and associated karstification is occurring and where it can be expected to occur. The process of gypsification, and ultimately sulphate dissolution, is requisite for the formation of porous dedolomite zones within the carbonates of the Prairie Evaporite Formation. Dedolomitized beds are recognized as aquifer units that are known to have contributed to Devonian-sourced, high-salinity water inflows to mine pits in the mineable oil sands area. Evidence is provided for a top-down advancement of halite and sulphate dissolution in all evaporites in northeastern Alberta. Circular, chain-like karst lakes are likely surficial expressions of the meteoric conduits for top-down karstification of sulphates east of the Prairie Evaporite halite dissolution scarp, similar to that observed in the well-documented sulphate karst district of Wood Buffalo National Park.
Kontribusi Virtual Community Indo Game Evolved Sebagai Wadah Komunikasi Kelompok Game Ark Survival Evolved Dengan Mengandalkan Media Sosial
Authors: Hubert Mansa , Irwansyah Irwansyah 
Published: March 2022

Manusia merupakan makhluk sosial yang membutuhkan interaksi dengan sesama masyarakat agar membentuk satu kesatuan, dengan kata lain terbukti bahwa seluruh khalayak mempunyai naluri untuk berkelompok atau bermasyarakat. Perkumpulan sejumlah individu atau komunitas dapat terwujud atas dasar persamaan ambisi dan komunikasi antar anggota. Pada masa ini telah terbukti bahwa era digital berkembang sangat pesat, salah satu bukti nyata yaitu tingkat perkumpulan secara maya melambung tinggi. Internet (Interconnected Network) dan media sosial berperan cukup penting untuk wadah berkomunikasi antarindividu, kedua hal tersebut merupakan salah satu kegunaan utama internet yaitu kesenangan atau penghibur. Permainan online merupakan salah satu kesenangan yang diberikan oleh internet, melalui permainan online siapapun mampu berinteraksi pada dunia maya. Beserta ini terjadi peningkatan terhadap pemain berasosiasi pada komunitas game. Proses Interaksi sosial terjadi melalui komunikasi antar anggota, spesifikasi karakter yang dimainkan serta dunia yang khalayak ciptakan. Penelitian ini memiliki tujuan untuk memahami kontribusi virtual community INDO GAME EVOLVED selaku. wadah komunikasi kelompok permainan online ARK SURVIVAL EVOLVED dengan menggunakan Whatsapp. Penelitian ini dilakukan dengan menggunakan pendekatan kualitatif, dengan metode pengumpulan data melalui systematica literature review dan dengan melakukan wawancara dengan informan diantaranya adalah Admin Server INDO ARK EVOLVED dan salah satu anggota kelompok virtual INDO ARK EVOLVED. Hasil dari penelitian ini menunjukan bahwa, kontribusi komunitas virtual sebagai wadah komunikasi para anggota komunitas INDO ARK EVOLVED dengan mengandalkan Whatsapp sangat efektif dalam penyampaian informasi. Pesan yang terkonstuksi diterima jelas oleh para anggota mulai dari informasi seputar game hingga ajang promosi jualan barang virtual. Kontribusi INDO ARK EVOLVED ditandai baik dengan bertahan dan berkembangnya komunitas hingga saat ini.
Kata Kunci: komunitas virtual; game online mobile; ark survival evolved; media sosial
Genetic diversity and phylogenetic analyses of Vitex agnus castus L. populations using ISSR-PCR and chloroplast DNA trnL intron and trnL-F sequences
Authors: Sevindik Emre 
Published: March 2022

In this study, genetic diversity and phylogenetic analysis of some Vitex agnus-castus L. populations were conducted based on ISSR-PCR and chloroplast DNA trnL intron, and trnL-F sequence analyses. Vitex agnus-castus populations were detected in Aydın province, Turkey. Fresh leaf samples from the populations were collected and brought to the laboratory for genomic DNA isolation. 15 ISSR primers were used to determine the genetic diversity ofe Vitex agnus-castus populations. A total of 138 bands were obtained in ISSR analysis, 85 of which were polymorphic and 53 were monomorphic. Polymorphism rate was determined as 61.59 %. trnC, trnD, trnE, and trnF primers were used for PCR amplification of the chloroplast trnL intron and trnL-F region. A total of 138 bands were obtained by ISSR analysis. For trnL intron analyses, nucleotide lengths of 13 populations were between 508 and 516. The average nucleotide composition consisted of 38.5 % T, 18.3 % C, 27.5 % A and 15.7 % G. In trnL-F assays, the nucleotide lengths of the 13 populations ranged from 330 to 353. The average nucleotide composition consisted of 29.4 % T, 18.1 % C, 32.9 % A and 19.6 % G. The results of the phylogenetic trees constructed using some trnL intron and trnL-F sequences of Vitex doniana, Vitex trifolia, Vitex triflora, Vitex turczaninowii, Vitex queenslandica, Vitex axillariflora, Vitex rotundifolia and Vitex negundo species obtained from NCBI were compared. As a result of the study, polymorphisms were obtained at a rate of 61.59% from the ISSR analysis. In addition, the phylogenetic relationship between chloroplast trnL intron and trnL-F sequences of Vitex agnus-castus populations along with the other species was revealed.
BENTUK PENYAJIAN MUSIK TARI NGAGAH IMO PADA MASYARAKAT KERINCI PROVINSI JAMBI DALAM WADAH ESTETIKA
Authors: Sepdwiko Deria 
Published: March 2022

<p><em>Penelitian ini membahas tentang bentuk penyajian tari ngagah imo pada masyarakat Kerinci Provinsi Jambi dalam Wadah Estetika. Terciptanya tari ngagah imo berangkat dari ritual yang dimiliki oleh masyarakat pendukungnya yaitu ritual pengobatan. Ritual tari ngagah imo berfungsi sebagai pengobatan suatu penyakit yang berhubungan dengan makhluk gaib. Metode yang dipergunakan dalam penelitian ini adalah metode kualitatif (deskriptif analisis), yaitu, ucapan, tulisan dan prilaku yang dapat diamati dari orang atau subjek itu sendiri yang dideskripsikan dan dianalisis dengan cara menyaksikan secara langsung pertunjukan tari ngagah imo, maupun melalui observasi, wawancara, dan dokumentasi yang berkaitan dengan penelitian. Ritual tari ngagah imo dipercayai oleh masyarakat Kerinci Provinsi Jambi sebagai sarana pengobatan yang berhubungan dengan makhluk gaib. Namun seiring dengan pergeseran waktu ritual ini dijadikan sebagai ide dalam menciptakan tari ngagah imo yang hanya berfungsi sebagai hiburan. Kehadiran tari ngagah imo ini mendapat tempat dalam kehidupan masyarakatnya, hal ini terlihat bahwa tari ngagah imo ditampilkan dalam berbagai acara-acara yang terdapat di Sulak deras Kabupaten Kerinci, seperti acara-acara yang diadakan oleh pemerintah Kabupaten Kerinci, sekolah-sekolah yang terdapat di Kerinci, event-event, maupun acara lainnya. Fokus penelitian yang dibahas dalam penelitian ini yaitu melihat estetika yang dihadirkan dalam tari ngagah imo secara keseluruhan dalam segi pertunjukan. Karena tarian ini berangkat dari ritual pengobatan yang bersifat sakral di lahirkan dalam bentuk seni tari. Kemajuan dan perkembangan zaman membuat masyarakat Kerinci Provinsi Jambi sangat memperhatikan perkembangan tari ngagah imo kedepannya agar tidak akan mengalami kepunahan.</em><em></em></p>