bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluconazole in Hypercalciuric Patients with Increased 1,25(OH)2D Levels: The Prospective, Randomized, Placebo-Controlled, Double Blind FLUCOLITH Trial.
Authors: Bertholet-Thomas Aurélia , Portefaix Aurélie , Flammier Sacha , Dhelens Carole , Subtil Fabien , Dubourg Laurence , Laudy Valérie , Bouar Myrtille Le , Boussaha Inesse , Ndiaye Mariétou , Molin Arnaud , Lemoine Sandrine , Bacchetta Justine 
Published: January 2022

Abstract
BackgroundHypercalciuria is one of the most frequent metabolic disorders associated with nephrolithiasis and/or nephrocalcinosis possibly leading to chronic kidney disease (CKD) and bone complications in adults. Orphan diseases with different underlying primary pathophysiology share inappropriately increased 1,25(OH)2D levels and hypercalciuria, e.g., hypersensitivity to vitamin D and renal phosphate wasting. Their management is challenging, typically based on hyperhydration and dietary advice. The antifungal azoles are known to inhibit the 1α-hydroxylase and therefore decrease 1,25(OH)2D levels; they are commonly used, with well described pharmacokinetic and tolerability data. Fluconazole has been successfully reported to reduce calciuria in patients with CYP24A1 or SLC34A3 mutations, with no safety warnings. Thus, based on these case reports, we hypothesize that fluconazole is effective to decrease and normalize calciuria in patients with hypercalciuria and increased 1,25(OH)2D levels.MethodsThe FLUCOLITH trial is a prospective, interventional, randomized in parallel groups (1:1), placebo-controlled, double blind trial. A total of 60 patients (10-60years) with nephrolithiasis and/or nephrocalcinosis history, hypercalciuria (> 0.1 mmol/kg/d), increased 1,25(OH)2D levels (> 150 pmol/L) and 25-OH-D levels >20 nmol/L, will be included. Inclusions will be performed only from mid-September to the beginning of February to avoid bias due to sunlight-induced vitamin D synthesis. The primary endpoint will be the proportion of patients with normalization of 24-hour calciuria between baseline and 16 weeks, or with a relative decrease of at least 30% of 24-hour calciuria in patients who still display at W16 a 24-hour hypercalciuria. DiscussionThe current challenge is to propose an efficient treatment to patients with hypercalciuria and increased 1,25(OH)2D levels in order to prevent later complications and notably CKD that can ultimately lead to end-stage renal disease. Based on improvement of knowledge in phosphate/calcium metabolism, pathophysiology and genetics, the “off-label” use of fluconazole was recently reported to be useful in hypercalciuric patients with increased 1,25(OH)2D levels. Thus, the FLUCOLITH study is a unique opportunity to develop a new indication of a well-known and not expensive drug in orphan renal diseases, the ultimate objective being the secondary prevention of CKD worsening in these patients. Trial RegistrationClinicalTrial.gov, ID: identifier: NCT04495608. Registered on July 23rd, 2020
Nitrophenyl-group-containing Heterocycles. Part I. Synthesis, Characterization, Anticancer Activity and Antioxidant Properties of Some New 5,6,7,8-tetrahydro-isoquinolines Bearing 3(4)-nitrophenyl Group
Authors: Sayed Eman M. , Hassanien Reda , Farhan Nasser , Aly Hanan F. , Mahmoud Khaled , Bakhite Etify A. 
Published: January 2022

Abstract
Regioselective cyclocondensation of 2,4-diacetyl-5-hydroxy-5-methyl-3-(3-nitrophenyl/4-nitrophenyl)cyclohexanones 1a,b with cyanothioacetamide afforded the corresponiing 7-acetyl-4-cyano-1,6-dimethyl-6-hydroxy-8-(3-nitrophenyl/4-nitrophenyl)-5,6,7,8-tetrahydro-soquinoline-3(2H)-thiones 2a,b in 93-96% yield. Reaction of compounds 2a,b with some ethyl iodide, 2-chloroacetamide (4a) or its N-aryl derivatives 4b-e by refluxing in ethanol, in the presence of slightly excess molar amounts of sodium acetate trihydrate, for one hour gave 3-ethylthio-5,6,7,8-tetrahydro-isoquinoline 3 and (5,6,7,8-tetrahydroisoquinolin-3-ylthio)acetamides 5a-i, respectively. On heating compounds 5b-d,f,g in ethanol containing a catalytic amount of sodium carbonate, they converted into their isomeric 1-amino- 6,7,8,9-tetrahydrothieno[2,3-c]isoquinoline-2-carboxamides 6b-d,f,g. Structural formulae of all synthesized compounds were characterized on the basis of their elemental analyses and spectroscopic data. Also, the biological evaluation of the synthesized isoquinolines as anticancer and antioxidant agents have been carried out and the obtained results are reported herein.
When Shape Matters: Using a Simple Mathematical Model to Estimate Critical Area Sizes in Conservation
Authors: de Assis Raul Abreu , Malavazi Mazilio Coronel , Pazim Rubens , Cannale Gustavo , Cecconello Moiseis , Fonseca Odair José Teixeira da 
Published: January 2022

Abstract
In the analysis of anthropogenic impact on the environment arises the question of whether the shapes of preserved habitat fragments play an important role in the conservation of wild species. In this work we use a very simple mathematical model based on a reaction-diffusion equation to analyze the effects of geometric shape and area on the permanence of populations in habitat fragments. Our results indicate that a dimensionless quantity calculated from a combination of biological variables is the main component that determines if the species survives in the preserved fragment and whether its geometric shape is important. We provide a methodology to calculate critical area sizes for which population size is most affected by fragment shape. The calculation is based on four quantitative variables: maximum per capita reproduction rate, per capita mortality rate outside the fragment, carrying capacity in the conserved environment and mobility in the disturbed environment. The methodology is illustrated by a preliminary study, in which the model is used to estimate threshold area sizes for habitat fragments for the threatened species Sapajus xanthosternos .
Description of laminar-turbulent transition of an airfoil boundary layer measured by differential image thermography using directed percolation theory
Authors: Wester Tom , Peinke Joachim , Gülker Gerd 
Published: January 2022

Abstract
Transition from laminar to turbulent flow is still a challenging problem. Recent studies indicate a good agreement when describing this phase transition with the directed percolation theory. This study presents a new experimental approach by means of differential image thermography (DIT) enabling to investigate this transition on the suction side of a heated airfoil. The results extend the applicability of the directed percolation theory to describe the transition on curves surfaces.

The experimental effort allows for the first time an agreement between all three universal exponents of the (1+1)D directed percolation for such airfoil application. Furthermore, this study proves that the theory holds for a wide range of flows, as shown by the various conditions tested. Such a large parameter space was not covered in any examination so far. The findings underline the significance of percolation models in fluid mechanics and show that this theory can be used as a high precision tool for the problem of transition to turbulence.
Achieving Proportional Representation In A Reproductive Health Survey Through Social Media: Process And Recommendations
Authors: McCarthy Ona L , Palmer Melissa J , Gubijev Anasztazia , Wellings Kaye , Mann Sue , Leon Lydia , Callaghan Faye , Patterson Sophie , French Rebecca S 
Published: January 2022

Abstract
Background: The narrative surrounding women’s reproductive health has shifted from a medical model to an emphasis on reproductive well-being over different life-stages. We developed and piloted a tracker survey for monitoring women’s reproductive health and well-being in England, recruiting respondents online. This paper reports on the success of the online recruitment strategies in achieving a sample proportionally representative of the England general population.Methods: Recruitment was through Facebook and Instagram advertisements and dissemination through Twitter and a blog. At the end week one, the sample was reviewed and compared to the 2011 Census England population. From week two, recruitment targeted under-represented groups. Key data were compared with prevalence estimates from the Third National Survey of Sexual Attitudes and Lifestyles (Natsal-3).Results: Between 1 July-17 August 2021, 13,962 people initiated the online survey, with 11,578 completing it. Numbers were low initially, but peaked at 1700 survey initiations per day after increasing the daily advertisement budget on day seven. At the end of week one, minority ethnic groups and people without a degree or equivalent were under-represented. From week two, we altered the advertisement settings to show to people whose profile indicated they were a ‘high school leaver’ had ‘up to some high school’, worked in industries that do not typically require a degree or lived in local authorities with a high proportion of ethnic minority residents. This had a modest effect, with the final sample short of proportional representation in terms of ethnicity and education but close in terms of region and age. Compared to Natsal-3, we found consistency in the proportion of respondents reporting an abortion and a live birth in the last year, however, the proportion of our sample reporting ever having experienced infertility was significantly higher than in Natsal-3, as was the proportion of ‘planned’ pregnancies in the last year.Conclusions: It is possible to recruit large numbers of respondents online, relatively quickly, to complete a reproductive health survey. This will be valuable to track reproductive health and well-being at a national level over time. More work is needed to understand reasons for non-response among under-represented groups.
Prevalence and Associated Factors of Chronic Kidney Disease Among Diabetic and Hypertensive Patients at Ambo Town Public Hospitals of West Shewa Zone, Oromia Region, Ethiopia
Authors: Hailu Haimanot Ewnetu , Dinku Belachew , Wondimu Jimmawork , Girma Bilisuma 
Published: January 2022

Abstract
Background: Chronic kidney disease is a global public health important disease that is associated with life threatening outcomes including renal failure and premature mortality unless diagnosed and treated promptly. Diabetes Mellitus and hypertension are the two major causes of chronic kidney disease worldwide. This study is aimed to determine prevalence and associated factors of chronic kidney disease among diabetes mellitus and hypertensive patients at Ambo town public hospitals, EthiopiaMethods: A cross-sectional study was conducted at Ambo University referral and general hospitals on 308 study participants. The participants were interviewed using interviewer administered questionnaire when they come for follow up at a chronic illness follow-up clinic. The patient charts were reviewed to retrieve information regarding medications, blood pressure, serum Creatinine and glucose level. A chronic kidney disease epidemiology collaboration equation was used to estimate Glomerular filtration rate from serum Creatinine. Data were analyzed SPSS version 23 for statistical analysis. Binary logistic regression analysis was used to identify factors associated with chronic kidney disease. Variables with a p-value below 0.2 at bivariable analysis were entered into the multivariable logistic regression model. Multivariable logistic regression analysis was used to examine the association between dependent and independent variable and p-value less than 0.05 was used to declare statistical significance.Results: A total of 308 diabetes mellitus and hypertensive patients were included in the study from Ambo town public hospitals. Of which 156 (50.6%) participants were female with mean (± SD) age of 47.15 ± 12.06 years. The prevalence of chronic kidney disease (stage 3–5) was 20.5% with (95% CI: 16%-25%). Long duration of hypertension (AOR=4.89, 95% CI=1.93-12.40), elevated systolic blood pressure (>140mmHG) (AOR=3.20, 95% CI=1.36-7.51), family history (AOR=3.36, 95% CI=1.56-7.24) and age greater than 55 years (AOR=2.17, 95% CI=1.09-4.31) were predictors of chronic kidney disease.Conclusion: The prevalence of chronic kidney disease was high. Older age, elevated SBP, long duration of hypertension and family history of kidney disease were independent predictors of chronic kidney disease. A preventive plan is mandatory to reduce the disease and complications in the community.
Climate Change and Child Health – Importance of Pediatricians for Parents
Authors: Lagally Lena , Schorlemmer Julia , Edlinger Maximilian , Schoierer Julia , Bose-O’Reilly Stephan 
Published: January 2022

Abstract
Background: Children are among the most vulnerable to suffer from health consequences due to climate change. Parents as caregivers play an important role in protecting them adequately. Pediatricians are regularly seen as highly-trusted health professionals, but it remains unclear whether they are an information source for parents regarding climate change and health. According to the Health Action Process Approach, parents’ risk perception is an important pre-intender for intention building. Methods: A sample of parents (N = 243) living in Germany completed an online, cross-sectional survey distributed in summer 2020. Regarding climate change and health, we asked about information sources, relevance estimation, and risk perception of parents. Multiple linear regression with general and individual risk perception as outcomes were used to analyze the effect of knowledge and personal relevance. Results: Parents seek information about climate change and health primarily via internet and social media. Pediatricians are rarely considered as suitable information source. Parents see the highest risk for their child through increasing air pollutants and stronger UV-radiation. Relevance (β = .52, t = 5.79, p < .001) and knowledge (β = .02, t = .36, p = .72) explain 18.2% of general risk perception (F(5, 208) = 9.25, p < .001, ΔR2 = .13). The effect is lower (13%) for individual risk perception (F(5, 189) = 5.67, p < .001, ΔR2 = .07). Conclusions: Pediatricians can play a valuable role in informing parents about climate change and health; nevertheless, they are not yet seen as suitable information sources by parents. Results demonstrate that knowledge about climate change and health is not sufficient to increase risk perception of parents, but strengthening risk perception is possible through rising relevance estimation. When informing parents about the expected health impact of climate change, this should be considered.
Association Among Spinnbarkeit, Electrical Conductivity, and Crystallization of Cervical Mucus and Pregnancy Rate in Egyptian Baladi Cows
Authors: Mohamed Mahmoud Yassin , El-Hafeez Ahmed Mohamed Abd , Ibrahim Mohamed El-said , Ramadan Mohamed Hussein , Amin Amin Mohamed , Helal Ahmed 
Published: January 2022

Abstract
Alteration of the biophysical and biochemical characteristics of cervical mucus (CM) due to differences in steroid hormones through the estrus cycle leads to different pregnancy rates. This investigation aims to study the possible relationship between CM properties with biochemical profiles, macro-mineral levels, and steroid hormones concentrations, and their effects on pregnancy rates of Egyptian cows. Fourteen Baladi cows were used and synchronized. The model log-likelihood ratio was highly significant (P=0.0009), and reported that the spinnbarkeit (SPK), electrical conductivity (EC), and crystallization (CRS) had significant effect on high pregnancy rate. The 3rd level of SPK (>13.5 cm) and EC (>15 mS/cm) was the highest significant (P=0.0016 & 0.0517, respectively) and a clear positive of estimate marginal effect (20.2543 & 10.6192, respectively) attitude towards the pregnancy rate. However, in case of the CRS, the significant effect was in the first two levels (P=0.0321 & 0.0425, respectively) with a high pregnancy rate, reverse the last 2 levels. Total protein, cholesterol, glucose, potassium, chloride levels, and estradiol concentrations were observed higher with increasing levels of SPK and EC and appearance of typical fern patterns (first two levels of CRS), in contrast to sodium, and progesterone concentrations that decreased with elevating levels of SPK and EC and appearance of atypical fern patterns (last two levels of CRS). There was a close correlation between CM properties and steroid hormones (P4 & E2). So, alterations in CM properties, especially SPK, EC, and CRS, can be utilized to foresee estrus time and, as a result, insemination time.
Clinical impact of C-reactive protein to albumin ratio of the 7th postoperative day on prognosis after laparoscopic colorectal cancer surgery
Authors: Kataoka Masahiro , Gomi Kuniyuki , Ichioka Ken , Iguchi Takuya , Shirota Tomoki , Makino Arano , Shimada Ko , Maruyama Kiyotomi , Mihara Motohiro , Kajikawa Shoji 
Published: January 2022

Abstract
Background/Aim:
C-reactive protein to albumin ratio (CAR) has been utilized as a prognostic factor in various carcinomas. We investigated the relationship between preoperative, first postoperative day (POD1), and seventh postoperative day (POD7) CARs and the prognosis of patients with colorectal cancer (CRC).
Patients and Methods:
320 patients with CRC who underwent laparoscopic radical resection between May 2011 and December 2016 were enrolled. Patients were selected into two groups, high CAR and low CAR, based on preoperative, POD1, and POD7 CARs. The relapse-free survival (RFS) and overall survival (OS) were compared between groups using propensity score matching.
Results
The high CAR group had a significantly worse RFS and OS (n=72/group, RFS: p<0.001; OS: p=0.002) at POD7 than those in the low CAR group. However, in preoperative and POD1 analysis, no differences were observed.
Conclusion
In patients with colorectal cancer, CAR of POD7 was a significant prognostic factor.
Clinical profile of patients with Seronegative celiac disease
Authors: Rahmanipour Elham , attarian fahimeh , Ghorbani Mohammad , Shahbazkhani Bijan , Ghavami Vahid , Ganji Azita 
Published: January 2022

Abstract
Background
Celiac disease (1) mostly diagnosed base on positive serology and duodenal mucosal atrophy, but some patients have negative serology and their diagnosis have some limitation, it delay in diagnosis likely accompanied a poor prognosis and high risk of developing complications of CD. The aim of this study was determent clinical profile of patients with Seronegative CD (SNCD).
Methods
in this retrospective study, 1115+8 patients, that evaluated for CD with mucosal atrophy included between 2010 to2020. All patients with IgA deficiency other IgG based serology for diagnosis of celiac was done and if these antibodies were negative consider as possible SNCD. If they had positive DQ2-DQ8, and clinical symptoms or had positive challenge test after12 months of GFD were considered as SNCD.
Results
of total 1115 patients 27 (2.4%) had seronegative mucosal atrophy of duodenum and diagnosed as a SNCD (96.2% marsh3), the mean age and BMI in SNCD patients were significantly higher than other CD patients (p<0.05).
Conclusion
The prevalence of SNCD was 2.4% that likely related to over weighting, so clinicians should be considered high possible of seronegative CD in patients with over weighting and mucosal atrophy of duodenum.
Genotype calling and haplotype inference from low coverage sequence data in heterozygous plant genome using HetMap
Authors: Gong Hao , Han Bin 
Published: January 2022

Abstract
Many software packages and pipelines had been developed to handle the sequence data of the model species. However, Genotyping from complex heterozygous plant genome needs further improvement on the previous methods. Here we present a new pipeline available at https://github.com/Ncgrhg/HetMapv1) for variant calling and missing genotype imputation from low coverage sequence data for heterozygous plant genomes. To check the performance of the HetMap on the real sequence data, HetMap was applied to both the F1 hybrid rice population which consists of 1495 samples and wild rice population with 446 samples. Four high coverage sequence hybrid rice accessions and two high coverage sequence wild rice accessions, which were also included in low coverage sequence data, are used to validate the genotype inference accuracy. The validation results showed that HetMap archived significant improvement in heterozygous genotype inference accuracy (13.65% for hybrid rice, 26.05% for wild rice) and total accuracy compared with other similar software packages. The application of the new genotype with the genome wide association study also showed improvement of association power in two wild rice phenotypes. It could archive high genotype inference accuracy with low sequence coverage with a small population size with both the natural population and constructed recombination population. HetMap provided a powerful tool for the heterozygous plant genome sequence data analysis, which may help the discover of new phenotype regions for the plant species with complex heterozygous genome.
Soil Nutrient Balance and Stock on Smallholder Farms at Agew Mariam Watershed in Northern Ethiopia
Authors: Esubalew Tilahun , Amare Tadele , Molla Eyayu 
Published: January 2022

Abstract
IntroductionSoil nutrient balance is used to evaluate the state of soil fertility, rate of nutrient depletion, sustainability of land productivity, the environmental wellbeing of an area, and to take appropriate management decisions. This study was conducted to quantify soil nutrient balance and stocks on smallholder farms at the Agew Mariam watershed in northern Ethiopia in the 2020/21 main season.MethodsInflows and outflows of nitrogen (N), phosphorus (P), and potassium (K) into, and out of barley, tef, and wheat farms were determined through, field measurement, laboratory analysis, and interviews. The balance quantification was done by subtracting nutrient outputs from the inputs. ResultsThe N partial balance of barley, tef and wheat was -66, -9.8, and -50.7 kg ha-1 yr-1 respectively. The P balance was also -5.9, 0.9, and -2.6 kg ha-1 yr-1 for barley, tef, and wheat respectively. Whereas, K balance was -12.3, -3.2, and -5.4 kg ha-1 yr-1 from barley, tef, and wheat respectively. The balance results revealed that N, P, and K had negative values except for P in tef. Grain yield and crop residue removal were the major paths of nutrient loss. The stock of N was 1295, 1510, and 1240 from barley, tef, and wheat kg ha-1respectively. While, the P stock was 63, 18.7, and 27.5, kg ha-1 from barley, tef, and wheat farms respectively. Similarly, K stock was 1092.7, 1059.4, and 1090.6 kg ha-1 from barley, tef, and wheat cropping systems respectively. Conclusions﻿Reversing the imbalance between inflows and outflows via adding organic and inorganic fertilizers is critically essential for the study area.