bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSXV-7 Nutrient Digestibility Evaluation in Finisher Pigs from Diverse Feed Efficiency Groups
Authors: Beens Laura , Mejicanos Gustavo A , Rajendiran Ethendhar , Yang Chengbo , Nyachoti Martin , Lei Huaigang , Knol Egbert , Bergsma Rob , Rodas Argenis 
Published: September 2022

Abstract
Feed efficiency (FE) is a critical trait in swine production to improve the overall production efficiency and profitability and is an important goal for pig breeding companies. FE is a complex trait involving utilization and storage of dietary nutrients in different organs, and more especially in skeletal muscles. However, whether nutrient digestibility in the pig is altered by genetic selection for FE is less clear. Thus, this study investigated the variation in apparent total tract nutrient digestibility (ATTD) in finisher boars selected for high (HFE) or low (LFE) FE within a Large White dam and sire line. A total of 130 boars were selected at 23 wk of age based on their genomic-enabled breeding value for FE and fed ad libitum a corn-soybean meal-based diet using feeding stations that recorded individual feed intake and body weight. Fecal and diet samples were analyzed for dry matter, neutral detergent fiber, acid detergent fiber, crude protein (CP), energy, ash, phosphorus, and calcium to determine ATTD using acid-insoluble ash as an indigestible marker. There was no difference in ATTD values between genetic lines (P &gt; 0.10). However, HFE pigs had greater (P = 0.03 ATTD of CP compared with LFE pigs. Based on the estimated protein deposition and the average feed intake one week before sampling, calculated dietary digestible lysine content diet met the requirements for the average pig. However, HFE pigs had a reduced feed intake, implying that they might have experienced a shortage of lysine in the diet. It is unclear whether the improved ATTD of CP was due to a lysine-shortage or differences in genetic merit for FE.
PSX-B-13 Effect of Nutragen Supplementation on Incidence of Bovine Respiratory Disease, Weight Gain and Culling Rate in Feedlot Receiving Cattle
Authors: Sueldo Gustavo , Humphrey Brooke , Bressan Elbio , Buisker Timothy 
Published: September 2022

Abstract
Bovine Respiratory Disease (BRD) is a major health issue in feedlot cattle negatively impacting growth performance and profitability. NutraGen (NG, Phibro Animal Health) is an immunomodulatory feed additive with demonstrated benefits in supporting the immune system in cattle during periods of stress. The aim of this study was to determine the effect of feeding NG on incidence of BRD during the receiving phase and the carryover performance during the full days on feed in a mixed population of feedlot cattle. Over 4 weeks, 5,125 head of mixed breeds and sex cattle from 4 regions in Argentina were weighed at arrival and assigned to treatment, CON (no NG, n=2,391) or NG (9 g/100 kg BW, n=2,734). Cattle were fed the treatments during the 30-d pre-conditioning phase only, and health and performance measurements recorded. Multivariant linear regression analysis was used to estimate the main effect of treatment. Cattle within treatment were further stratified by travel distance. After controlling for breed, sex, arrival body weight (BW), and pen size, NG cattle were 2.3% less likely to contract BRD (P&lt; 0.01), 2.1% less likely to be discarded (P&lt; 0.01), and gained 7.3 kg more BW than CON. NG had a greater impact on cattle that traveled longer distances. NG fed cattle traveling 630-880 km to the feedlot were 7.2% less likely to contract BRD (P&lt; 0.01), 7.5% less likely to be discarded (P&lt; 0.01) and gained 8.5 kg more BW compared with CON. NG fed cattle traveling over 880 km (n=1219) were 8.8% less likely to contract BRD (P=0.02), 8.7% less likely to be discarded (P&lt; 0.01) and gained 17.5 kg more BW (P&lt; 0.01) than CON cattle. These data support the use of NG supplementation during the pre-conditioning feedlot period to improve health and performance, especially for cattle transported over long distances.
PSXI-20 Evaluation of the Effects of Hempseed Cake on Immune Parameters in Response to an Endotoxin Challenge in Finishing Steers
Authors: Winders Thomas , Neville Bryan W , Swanson Kendall C 
Published: September 2022

Abstract
Hempseed cake, a byproduct of industrial hemp and subsequent hempseed oil extraction processes, contains cannabinoids and fatty acids that could be potentially therapeutic for livestock, but little is known about the extent to which hempseed cake can influence immune response variables of cattle. An endotoxin challenge experiment using crossbred steers (n = 5; initial BW = 542 kg, SD = 40 kg) was conducted to evaluate the effects of hempseed cake treatment (HEMP) and dried corn distillers grains plus solubles treatment (DDGS; each included at 20% of diet DM in respective treatments) in comparison with a dry-rolled corn-based negative control treatment (CON) on plasma glucose, non-esterified fatty acids (NEFA), urea nitrogen (PUN), rectal temperature, twelve cytokine (IFNγ, IL-1α, IL-6, IL-8, IL-10, IL-36RA, IP10, MCP-1, MIP-1α, MIP-1β, TNFα, and VEGF-A), and amino acid concentrations at five timepoints after administration of endotoxin (lipopolysaccharide; LPS). Steers were administered LPS at 0.25 μg/kg BW, and blood was collected via jugular venipuncture before and 1, 2, 4 and 6-hours post-bolus injection. Data were analyzed as repeated measures using the MIXED procedure of SAS, and pre-bolus values was used as a covariate. A treatment by hour interaction (P = 0.04) was observed for PUN, and pre-bolus PUN was greatest (P &lt; 0.01) in steers fed the HEMP. Plasma IL-1α, IL-36RA, and TNF-α were least (P ≤ 0.02), while IL-10 and MIP-1α tended (P ≤ 0.10) to be less in steers fed the HEMP diet. Plasma isoleucine, leucine, tryptophan, and tyrosine concentrations were greatest (P ≤ 0.04) in steers fed the DDGS diet, plasma aspartic acid and glycine concentrations were greatest (P ≤ 0.02) for steers fed the DDGS and CON diets. Overall, these data suggest that hempseed cake has potential to reduce inflammation by influencing cytokine production, but more research is needed to further understand animal growth performance and health implications.
PSI-9 Disrupting Cxcl12-Cxcr4 Chemokine Axis at the Fetal-Maternal Interface During Implantation Results in Altered Expression of Angiogenic and Insulin-Like Growth Factors in Ovine Placenta Midgestation
Authors: Wasson Megan E , Mason Kaitlyn M , Ashley Ryan L 
Published: September 2022

Abstract
Placental dysfunction is a fundamental cause of intrauterine growth restriction, and fetal morbidity and mortality in livestock. Though causes of placental dysfunction are not well characterized, origin of disease transpires during placental development (placentation). The C-X-C motif chemokine ligand 12 (CXCL12) working through its receptor CXCR4 is implicated in placentation but precise roles of this axis are unclear. Using an in vivo sheep model, we demonstrated that suppressing CXCL12-CXCR4 signaling at the fetal-maternal interface reduces placental vascularization. We hypothesized these negative impacts early in gestation would result in compromised placental development and vascularization at midgestation. To test, onday 12 post-breeding, osmotic pumps were surgically installed in 22 ewes and delivered either CXCR4 inhibitor (AMD3100) at 1.5X dose, (n=8) or 3X dose (n=8) or saline (n=6) into the uterine lumen ipsilateral to the corpus luteum for 14 days. On day 90 placental tissues were collected and maternal and fetal placenta components separated and analyzed. The objectives were to determine if suppressing CXCL12-CXCR4 actions at the fetal-maternal interface during implantation results in reduced placental and vascular development at midgestation. Gene expression for angiogenic factors and members of the insulin-like growth factor family were analyzed using Real-time qPCR. Of the targets tested, mRNA expression was significantly altered by AMD3100 treatment for the vascular endothelial growth factor receptors (FLT-1 and KDR) primarily in maternal placenta. Expression of insulin-like growth factors 1 and 2, IGF-binding protein 3, and placental lactogen were significantly altered in fetal placenta with AMD3100 treatment compared to control. Our preliminary data underscore the importance of CXCL12-CXCR4 signaling during placentation and provide strong evidence that altering CXCL12-mediated signaling during early placentation induces enduring placental effects manifesting later in gestation. A greater understanding of CXCL12/CXCR4 functions may reveal methods to improve reproductive success and fetal health.
PSIII-13 Performance and Resiliency of Lactating Does and Their Kids in Woodlands with Supplemental Feedstuffs or Grazing
Authors: Shrestha Bhuwan , Karki Uma , Chaudhary Santoshi , Tiwari Anand , Karki Lila B 
Published: September 2022

Abstract
Previous woodland-grazing studies reported poor performance of young, growing goats and recommended for supplemental feed. However, not much is known about strategies to supplement lactating animals stocked in woodlands. The objective of this study was to evaluate the performance and resiliency against gastrointestinal (GI) parasites of lactating does and their kids stocked in woodlands with supplemental grazing or feedstuffs. The study was conducted in six woodland plots and adjacent silvopasture plots were used for supplemental grazing. Seventeen lactating Kiko does with their kids (33) were divided into two groups. Each group was assigned to separate sets of woodland plots and rotated among those plots throughout the study period. One group (Group 1) was allowed to supplemental grazing in adjacent silvopasture plots for 3-4 hours daily and another group (Group 2) was supplemented with ad libitum hay and corn (0.5% of metabolic weight). Animal performance data (live weight, FAMACHA score, and body condition score (BCS)) were collected at the beginning after a 5-day-adjustment period, fortnightly during the study, and at the end. Vegetation samples from woodland and silvopasture plots were collected and analyzed for biomass and quality (CP, ADF, NDF, TDN). Fecal samples were collected and analyzed for GI parasites from animals having FAMACHA score 3 and higher. Animal performance and forage quality data were analyzed using GLM procedure, forage biomass data using Mixed model, and fecal egg-count data using the non-parametric (NPAR1WAY) procedure in SAS 9.4. Silvopasture produced greater biomass (60%) with higher quality (p&lt; 0.0001) vs. woodland. Group-1 does showed better FAMACHA score and less prevalence of GI-parasite infestation vs. Group-2 does (p&lt; 0.05). Group-1 kids had lower coccidia count vs. Group-2 kids (p&lt; 0.05). In conclusion, supplemental grazing was better for resiliency against GI parasites in lactating does and young kids than supplemental feeding.
PSIV-A-10 Effects of Canola Meal Inclusion in Gestation and Lactation Diets with or without Probiotic on sow Performance, Milk Composition and Piglet Performance
Authors: Zhang Xiaoxiao , Holanda Debora Muratori , Nyachoti Martin , Lee Jinyoung 
Published: September 2022

Abstract
The experiment was conducted to determine the effects of high dietary canola meal (CM) inclusion in gestation and lactation diets with or without probiotic supplementation on sow and litter performance and milk composition. Seventy-five sows were randomly allotted 1 of 3 diets to give 25 replicates per treatment. Diets consisted of a corn and soybean meal (SBM) control (CTRL) or the CTRL diet with SBM replaced by 300g/kg CM and fed with (CM+) or without (CM-) probiotic ActisafÒ Sc 47 supplementation. Sows BW and backfat thickness were determined on d 80 and 111 of gestation, d 1 post-farrowing, and at weaning on d 21. Milk samples were collected on d 1 and d 21 post-farrowing to determine milk composition. Piglets were weighed on d 0 and at weaning. Data were analyzed using the PROC MIXED procedure of SAS 9.4 for a randomized complete block design. Dietary treatment had no effect on sow performance, milk crude protein and lactose oligosaccharide composition, piglet ADG and weaning weight, and piglet survivability (P &gt; 0.10). Sows fed CM- diet tended to fewer (P = 0.07) weaned piglets compared to sows fed CTRL diet, but no difference was found between sows fed the CM+ and CTRL diets (P &gt; 0.10). At weaning, milk fat content for sows fed with CM+ diet was higher (P &lt; 0.05) and tended to be higher (P = 0.09) compared with those fed the CTRL and the CM- diet, respectively. In conclusion, including 300g/kg CM in gestation and lactation sow diets supported similar sow and piglet performance as those the control diet, without affecting piglet survivability at weaning. Also, probiotic supplementation in CM diet increased sow milk fat content at weaning.
PSV-A-12 The Influence of Calcium Dose and Olive Meal on in-Vitro Rumen Fermentation Characteristics
Authors: Loh Huey Yi , Tangredi Briana V , Thorndyke Meghan P , de Bisneto Octavio Almeida Guimaraes , Osborne Devin , Zuchegno Sophie M , Zuvich Miranda L , Athens Courtney M , Engle Jerica A , Gifford Ryan J , Engle Terry E 
Published: September 2022

Abstract
Rumen fluid from three beef steers (480 ± 10 kg), fitted with rumen canulae, were used to investigate the impact of Ca dose and olive meal on in vitro rumen fermentation characteristics. Steers were fed a high concentrate finishing diet for 21d, and rumen fluid was collected from each steer 2h post-feeding. A 2 x 4 factorial arrangement of treatments was used for this experiment. Factors included: 1) 0 or 5% olive meal and 2) Ca dose: 0, 0.02, 0.04, and 0.08% Ca from CaCl2. A McDougall’s buffer-rumen fluid mixture (1:1; 30 mL) was added to conical tubes containing 0.5g of the ground basal diet and incubated at 39°C for 0, 4, 8, and 12h (5 replicates per treatment per time point). After incubation, supernatant was removed for VFA analysis and the remaining digesta was dried to determine DM disappearance (DMD). At 4 and 8h post incubation digestion tubes containing 0.04% Ca had greater (P &lt; 0.001) DMD when compared to all other Ca doses. At 12h post incubation, DMD was greater (P &lt; 0.001) in digestion tubes containing 0.02% and 0.08% Ca compared to all other Ca doses. At 8h post incubation, molar proportions of acetic acid were greater (P &lt; 0.03) in digestion tubes containing olive meal compared to no olive meal and were greater (P &lt; 0.001) in digestion tubes containing 0.08% Ca compared to all other Ca doses. At 12h post incubation, isobutyric acid (P &lt; 0.01) and butyric acid (P &lt; 0.02) were greater in digestion tubes containing 0.02% and 0.04% Ca compared to all other Ca doses. Butyric acid was lesser (P &lt; 0.02) with olive meal inclusion at 12h. Total VFA concentrations were similar across treatments. These data suggest that Ca and olive meal can impact in vitro fermentation characteristics.
PSV-A-10 Single-Cell Atlas of Bovine Skeletal Muscle Identifies Mechanisms Regulating Intramuscular Adipogenesis and Fibrogenesis
Authors: Wang Leshan , Li Chaoyang , Liu Qianglin , Li Yuxia , Gao Peidong , Zhang Xujia , Welborn Matthew , Fu Xing 
Published: September 2022

Abstract
Human and mouse studies have shown that fibro/adipogenic progenitors (FAPs) are a major source of intramuscular fat (IMF) and extracellular matrix (ECM) proteins. IMF and ECM proteins directly influence the palatability of beef, suggesting an essential role of FAPs in beef quality determination, which is still largely unexplored. We performed single-cell RNAseq (scRNAseq) using cells isolated from full blood Wagyu and Brahman cattle and Wagyu/Brahman cross cattle, which identified 21 cell clusters representing FAPs, several endothelial cell types, vascular smooths muscle cells, satellite cells, muscle fibers, and multiple immune cell types. More abundant FAPs were identified in the muscle of Brahman cattle, while a larger number of endothelial cells were identified in the muscle of Wagyu cattle. Further analysis of FAPs identified multiple FAP subpopulations with distinct gene expression profiles and anatomic locations. GSEA analysis revealed adipogenic and fibrogenic FAP subpopulations. A comparison of FAP subpopulations among different breeds showed higher complement system activity in the adipogenic FAP subpopulation of Wagyu cattle. Forced activation of the complement system in FAPs enhanced their adipogenic efficiency in vitro. In addition, cell-cell communication analysis identified active interactions between FAPs and other cell types through direct contact and secreted factors, many of which may affect FAP activities. In conclusion, our study revealed the single-cell atlas of bovine skeletal muscle and identified mechanisms regulating bovine intramuscular adipogenesis and fibrogenesis.
PSV-A-11 The Effect of Days on Feed for Feedyard Performance, Health, Carcass, and Organ Characteristics of Angus X Holstein Heifers
Authors: Husz Taylor C , Word Alyssa B , Karr Kendall J , Holland Ben P , Lawrence Ty E , Walter Lee-anne J , Hutcheson John P 
Published: September 2022

Abstract
The objective of this study was to evaluate the effect of days on feed (DOF) on performance, health, carcass, and organ characteristics of composite Angus x Holstein heifers (n = 3,676). At time of arrival (d 0), heifers from a single source were placed into large (~300 animal) pens at two commercial feedlot locations in the Texas Panhandle. At administration of final implant (Revalor® 200; d 240), heifers, within lot, were randomly assigned to one of four smaller pens, creating a block (location A block n = 6, location B block n = 7). Pens within blocks were randomly assigned to treatment (323, 344, 365, or 386 DOF). Data were analyzed using DOF as the fixed effect; block was nested within location as a random effect. Linear and quadratic effects were also tested; pen was experimental unit (n = 52). Heifer DMI did not differ across DOF (P = 0.72). Heifer ADG decreased linearly (1.52, 1.37, 1.31, and 1.27 kg/d; P ≤ 0.01). Heifer HCW (372, 387, 401, and 414 kg), longissimus dorsi muscle area (88.63, 89.55, 90.80, and 91.95 cm2), marbling score (535, 553, 563, and 594) and percentage of Prime QG (6.6, 11.2, 12.8, and 19.0 %) linearly increased (P ≤ 0.01) as DOF increased. Frequency of liver scores (edible, minor, or major abscess) did not change over DOF (P ≥ 0.82). Frequency of ideal heart score linearly decreased with DOF (53.0, 44.3, 25.7, and 15.0 %; P ≤ 0.01). Frequency of YG 3 did not change (P = 0.68), however YG 4 linearly increased with DOF (6.2, 12.0, 14.0, and 22.5 %; DOF P ≤ 0.01). Data collected in this trial indicate that DOF led to increased HCW, LM area, marbling, YG, and QG with minimal changes to DMI or liver scores for Angus x Holstein heifers.
PSV-A-6 Poor Maternal Nutrition During Gestation Decreases Shear Force and Alters Gene Expression Related to Fibrogenesis
Authors: Kawaida Mia Y , Alambarrio Daniela A , Gonzalez John M , Tillquist Nicole M , Reiter Amanda S , Smith Brandon I , Zinn Steven A , Govoni Kristen E , Reed Sarah A 
Published: September 2022

Abstract
To test the hypothesis that poor maternal nutrition during gestation would alter offspring muscle shear force and adipogenesis, fibrogenesis, and myogenesis mRNA expression, Dorset ewes (n=46) pregnant with twins were fed 100%, 60% or 140% of NRC requirements from d30±0.02 of gestation until parturition. Male offspring from CON (n=12), OVER (n=13), and RES (n=21) ewes were euthanized at d284±1.82 and longissimus muscle (LM) was harvested for shear force and gene expression. Vacuum-sealed LM steaks were aged for 10 d postmortem (4℃) and tenderness was measured using Warner-Bratzler shear force (WBSF) methodology. RNA was isolated from the LM to quantify mRNA expression of genes related to adipogenesis (fatty acid binding protein 4, CCAAT/enhancer-binding protein α, and peroxisome proliferator-activated receptor γ), fibrogenesis [collagen A1A (COLA1A), COL3A1, cystain-c, fibronectin (FN1), and lysyl oxidase (LOX)], and myogenesis [bone-morphogenic protein (BMP)-1, myostatin, myogenin, and myogenic differentiation 1). The WBSF decreased by 21% and 18% in OVER relative to CON (P=0.01) and RES (P=0.03), respectively, but CON and RES were not different (P=0.60). Expression of BMP1 was 1.5- and 1.8-fold greater in RES than OVER and CON, respectively (P≤0.03). Expression of FN1 was 1.6-fold greater in RES than OVER (P=0.02). Expression of COL1A1 was 2.2-fold greater in RES than CON (P=0.05) and was 1.6-fold greater in RES than OVER (P=0.07). Expression of LOX was decreased 37% in OVER compared with CON (P=0.04) and decreased 21% compared with RES (P=0.08). An effect of diet was not observed for other genes (P≥0.18). In summary, RES offspring had greater expression of genes related to fibrogenesis, but no observed difference in tenderness relative to CON. Over-feeding during gestation increased meat tenderness, which may be due to reduced collagen cross-linking by LOX.
PSV-A-13 Using Canine Olfaction to Detect Bovine Respiratory Disease: An Analysis of two Dogs’ Performance
Authors: Juge Aiden E , Hall Nathaniel , Richeson John T , Daigle Courtney L 
Published: September 2022

Abstract
Bovine respiratory disease (BRD) detection is a major challenge in feedlot cattle management. In a pilot study, two dogs were trained using sets of three samples to identify nasal swabs from cattle that developed signs of BRD after feedlot arrival. Cattle arrived at the feedlot in four lots and included a mixture of bulls (n=306) and steers (n=89). In the final stage of training, Dog A accuracy was 0.82 (95% CI: 0.79-0.85) and Dog B accuracy was 0.63 (95% CI: 0.60-0.67). A Kruskal-Wallis test identified differences in accuracy across lots (P &lt; 0.001). Differences between pairs were identified by Wilcoxon tests. Accuracy for Lot 2 (0.53, 95% CI: 0.46-0.59) was lower than all other lots. The greatest accuracy, 0.96 (95% CI: 0.92-1.00), was for sets where the positive sample came from a different lot than both negative samples, and differed from accuracy for all lots except Lot 4. A Wilcoxon test detected no differences (p = 0.76) in accuracy between sets that consisted of all bulls (0.73, 95% CI: 0.69-0.76) and sets that were a mixture of bulls and steers (0.72, 95% CI: 0.69-0.75). After training, in a double-blind detection test, unfamiliar samples (n=123) were presented to the dogs as 41 sets. Each set was evaluated twice by each dog. Accuracy for Dog A was 0.39 (95% CI: 0.28-0.50), no better than chance; however, Dog B’s accuracy was 0.45 (95% CI: 0.34-0.56), better than the 0.33 expected by chance. In the detection test, neither sample lot (p = 0.33) nor sex (p = 0.11) impacted performance for either dog. Dogs’ consensus accuracy was 0.54 (95% CI: 0.38-0.69). Overall, dogs showed some ability to discriminate between BRD-affected and healthy cattle using nasal swabs, however multiple compounding factors may have influenced canines’ decision making in this trial.