bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-composting of agricultural waste from spring onions, chicken manure, and biowaste
Authors: Oviedo-Ocaña Ricardo , Hernández Angélica , Dominguez Isabel , Soto-Paz Jonathan 
Published: March 2022

Abstract
Composting is an adequate method for treating and valorizing agricultural waste such as those from Spring onions (SO) cultivation and chicken breeding (chicken manure -CM). However, the low content of Total Organic Carbon in the waste from SO and the high concentration of total nitrogen in CM are limitations for the composting process. This research studied the co-composting of SO and CM in a paramo ecosystem, together with locally available co-substrates such as biowaste (Bw) and woodchips (Wc), focusing on the effect of co-composting in process development and end-product quality. A pilot-scale experiment was carried out using three treatments in triplicated composting piles: i) Treatment A: 43%CM+41%Bw+16%Wc (dry weight); ii) Treatment B: 52%CM+ 32%SO+16%Wc (dry weight), and iii) Treatment C: 70%SO + 30%Wc (dry weight). Treatments A and B reached thermophilic temperatures after two days of the process start and remained at that level for 17 days. However, treatment B reached environmental temperature during curing in a shorter time (43 days) than treatment A (53 days). Treatment C did not achieve thermophilic temperatures. Tests carried out at the end of the process showed end-product stability and non-phytotoxic characteristics (germination indexes 80%). From the perspective of agricultural use, products from the three treatments had limitations due to deficiencies in essential nutrients like phosphorus. Still, they had potential as a soil amendment for restoration processes. The fertility index of the products showed that treatments A and B presented values of 4.3 (over 5.0) while treatment C obtained a value of 2.5.
Cell Type Specific Cannabinoid CB1 Receptor Distribution Across the Human and Non-Human Primate Cortex
Authors: Chou Shinnyi , Ranganath Teijis , Fish Kenneth , Lewis David , Sweet Robert A 
Published: March 2022

Abstract
Alterations in cannabinoid CB1 receptor (CB1R) are implicated in various psychiatric disorders. CB1R participates in both depolarization induced suppression of inhibition (DSI) and depolarization induced suppression of excitation (DSE), suggesting its involvement in regulating excitatory and inhibitory (E/I) balance. Prior studies examining neuronal cell type specific CB1R distribution have been conducted near exclusively within rodents. Identification of these distribution patterns within the human and non-human primate cortex is essential to increase our insight into its function. Using co-labeling immunohistochemistry and fluorescent microscopy, we examined CB1R protein levels within excitatory and inhibitory boutons of human and non-human primate prefrontal cortex and auditory cortices, regions involved in the behavioral effects of exogenous cannabinoid exposures. We found that CB1R was present in both bouton populations within all brain regions examined in both species. Significantly higher CB1R levels were found within inhibitory than within excitatory boutons across all regions in both species, although the cell type by brain region interactions differed between the two species. Our results support the importance of conducting more in-depth CB1R examinations to understand how cell type and brain region dependent differences contribute to regional E/I balance regulation, and how aberrations in CB1R distribution may contribute to pathology.
Effect of pulse current on droplet transfer behavior and weld formation of 304 stainless steel in local dry underwater MIG welding
Authors: Liao Haipeng , Zhang Wenxu , Li Xuyan , Pei Kai , Lin Sanbao , Tian Jiyu , Wang Zhenmin 
Published: March 2022

Abstract
In this study, 304 stainless steel which widely used in the industrial field due to its excellent thermostability, corrosion resistance and welding performance, was welded using local dry underwater pulse metal inert-gas (LDU-PMIG) welding technology. The effect of different pulse peak currents on droplet transfer behavior and weld formation was studied. The results show that short circuit transfer, globular transfer, and projected transfer could be observed in the LDU-PMIG process. With the peak current of 240A, the droplet transfer mode was short circuit transfer and globular transfer, the weld formation was irregular with large particle spatters around the weld. With the peak current increased to 260A, the droplet transfer mode changed into the globular transfer, and the weld formation quality was improved. With the peak current of 280A, the droplet transfer mode was projected transfer, the weld formation was satisfied with few spatter, and the coefficient of weld formation was the largest, projected transfer was the most stable mode. With the increase of the peak current from 300A to 320A, the droplet transfer mode did not change, but the instability of the welding arc resulted in poor weld formation with serious spatters and undercuts.
Children and Neonates Anesthesia in Magnetic Resonance Environment in Italy. An active Call Survey
Authors: Sbaraglia Fabio , Spinazzola Giorgia , Adduci Alessia , De Riso Mariella , Festa Rossano , Garra Rossella , Tosi Federica , Rossi Marco 
Published: March 2022

Abstract
Background Pediatric anesthesia care in the Magnetic Resonance Imaging is a challenge for clinicians. The recent debate about the role of anesthetic agent on neural development, encouraged an evaluation of their actual activity in this environment. In this active call survey, the authors sought to delineate the Italian situation regarding national centers, staff involved, monitoring and tools available.Methods A complete sample of all national centers performing almost a pediatric discharge in the 2014 was obtained from Health Ministery registers. All Institution were contacted for a prospective phone investigation and a three section survey was fill out with the Physician in charge. A descriptive and exploratory analyses about the organization setting of the Centers (procedures, performers, monitoring and tools) were performed. Results Among 876 Institution screened, only 106 (37%) met minimal criteria for inclusion. Children are managed by anesthesiologists in the 95% of cases, while neonates in the 54%. A dedicated nurse is present in 74% of centers. While a pulse oximetry is present in 100% of centers, the rate of prevalence of other monitoring is lower (EKG94%; EtCO283%; Blood Pressure65%). A specific MRI-compatible ventilator is available in the 95% of Centers, but many tools are not equally homogenously distributed: Halogenated Vaporizers (82%), Scavenger System (72%), Suction devices (95%), non-magnetic infusion pumps (38%). Conclusions Our results could be a starting point to evaluate the further evolution of approach to children and neonates in magnetic resonance setting. Staff involved is generally qualified, but monitoring availability is unsatisfactory in many Centers.
β-Elemene Enhance the Cisplatin Sensitivity of Oral Squamous Cell Carcinoma via Inhibiting JAK2/STAT3 Pathway in Vitro and in Vivo
Authors: Wang Haiye , Ma Yingyi 
Published: March 2022

Abstract
Objective To investigate the effect of β-Elemene (β-Ele) on the cisplatin sensitivity of OSCC cells and its mechanism in vitro and in vivo. Methods The human OSCC cell lines Tca-8113 and the cisplatin-resistant cell line Tca-8113-CDDP were cultured with β-Ele or/and cisplatin. MTT assay was used to detect the cisplatin or β-Ele cytotoxicity and the cell viability. The cell cycles and cell apoptosis were detected by the flow cytometry. The expression of caspase-3, Bax, Bcl-2, STAT3, p-STAT3, JAK2, p-JAK2 were detected by western blot. The xenograft tumor model of OSCC was established in nude mice and treated with cisplatin and/or β-Ele. The volume and weight of the transplanted tumor was measured, and the expression of p-JAK2 and p-STAT3 in the xenograft tumor tissues were determined by immunohistochemistry and the cell apoptosis were detected by TUNEL assays.Results The combination of β-Ele and cisplatin significantly suppressed the cell proliferation, induced cell cycle arrest, promoted the apoptosis of Tca-8113-CDDP cells, and suppressed the activation of JAK2/STAT3 signaling pathway.The rescue experiments suggested that β-Ele enhanced cisplatin sensitivity via down-regulating JAK2/STAT3 signaling pathway. In vivo, β-Ele and cisplatin synergistically suppressed the tumor growth and induced apoptosis, and down-regulated the expression of p-JAK2 and p-STAT3. Conclusions β-Ele inhibits the cell viability and enhances the cisplatin sensitivity of OSCC by blocking the activation of JAK/STAT3 signaling pathway in vitro and in vivo, and the combination of β-Ele and cisplatin maybe a novel treatment for OSCC.
Automated harvesting by a dual-arm fruit harvesting robot
Authors: Yoshida Takeshi , Onishi Yuki , Kawahara Takuya , Fukao Takanori 
Published: March 2022

Abstract
In this study, we propose a method to automate fruit harvesting with a fruit harvesting robot equipped with robotic arms. Given the future growth of the world population, food shortages are expected to accelerate. Since much of Japan’s agriculture is dependent on imports, it is expected to be greatly affected by this upcoming food shortage. In recent years, the number of agricultural workers in Japan has been decreasing and the population is aging. As a result, there is a need to automate and reduce labor in agricultural work using agricultural machinery. In particular, fruit cultivation requires a lot of manual labor due to the variety of orchard conditions and tree shapes, causing mechanization and automation to lag behind. In this study, a dual-armed fruit harvesting robot was designed and fabricated to reach most of the fruits on joint V-shaped trellis that was cultivated and adjusted for the robot. For the fruit harvesting robot to harvest the fruit, it uses sensors and computer vision to detect and estimate the position of the fruit, and then inserts end-effectors into the lower part of the fruit. During this process, there is a possibility of collision within the robot itself or with other fruits depending on the position of the fruit to be harvested. In this study, inverse kinematics and a fast path planning method using random sampling is used to harvest fruits with robot arms. This method makes it possible to control the robot arms without interfering with the fruit or the other robot arm by considering them as obstacles. Through experiments, this study showed that these methods can be used to detect pears and apples outdoors and automatically harvest them using the robot arms.
Prevalence and Determinants of Antimicrobials Resistance of Pathogens Among Cancer Patients in Intensive Care Units
Authors: Mohamed Nancy , Ghazal Abeer , Ahmed Asmaa Abdel Hameed , Zaki Adel 
Published: March 2022

Abstract
Background: Infections caused by multidrug-resistant organisms (MDRO) are a global increasing threat among critically ill patients, especially those with underlying malignancy. We aimed to assess the prevalence, susceptibility patterns of MDRO among cancer patients in intensive care units (ICU) and their predictors.Methods: Over 4 years, we retrospectively reviewed medical records of 497 malignancy patients in ICU of a tertiary hospital in Egypt. Data for various factors like demographic characters, comorbidities, causative pathogen, and antimicrobial resistance (AMR) were collected and analyzed using univariate analysis. Logistic multivariate regression analysis was used to develop a clinical prediction model to estimate the probability of developing MDRO among this population. Results: A total of 748 isolates were obtained from 1249 collected clinical specimens. Gram-negative bacterial pathogens detected were 459 (61.4%) of all isolates, while 75 (10%) only were Gram-positive and 214 (28.6%) were fungal pathogens. The most frequent isolate was Klebsiella pneumoniae (n= 183), of which 107 were carbapenem resistant (CR) and 62 were extended spectrum beta lactamase (ESBL) strains. This was followed by Escherichia coli (n= 136), of which 17 were CR and 100 were ESBL strains while 3 were quinolone resistant. Furthermore, Acinetobacter baumannii (n= 67), of which 63 were CR. Overall susceptibility of gram-negative pathogens to antibiotics was recorded highest among colistin (97.3%). The prevalence of methicillin- resistant Staphylococcus aureus (MRSA) and Enterococcus species among Gram-Positive bacteria were 54.6% and 33.3% respectively, with no resistance reported to vancomycin, teicoplanin or linezolid. Among MDRO acquisition predictors were neutropenia, recent antibiotics use and receiving chemotherapy. Neutropenia had the highest odds ratio (O.R: 2.3, C.I: 1.28- 4.09) followed by recent antibiotics use (O.R: 1.8, C.I: 1.22- 2.59).Conclusion: Gram-Negative Bacilli were the most frequent reported MDRO, with resistance to higher generation cephalosporins and even carbapenems, limiting antibiotic treatment options to older class of antibiotics as colistin with potential side effects as nephrotoxicity. Estimating AMR probability using the prediction model of risk factors as neutropenia and previous antibiotics use, maybe functional in rapid identification of higher risk patients.
Delayed diagnosis of thoracic esophageal rupture due to blunt abdominal trauma without chest trauma: A case report
Authors: Han Jung Wook , Kim Jae Jun 
Published: March 2022

Abstract
Background
Thoracic esophageal rupture due to blunt trauma is very rare. Moreover, there have been no reports of thoracic esophageal rupture due to blunt abdominal trauma without chest trauma.
Case presentation:
We describe a rare case of esophageal rupture due to blunt abdominal trauma in a young female patient. Operation was delayed due to a misdiagnosis of chylothorax, and esophageal repair was performed six days after trauma. Postoperative esophageal leak developed and was treated with esophageal stent. She was discharged two months after surgery without sequelae.
Conclusions
It is important to consider esophageal rupture as a differential diagnosis even in patients with only abdominal trauma, when in doubt.
Follicular Fluid Steroid Levels and Mitochondrial Function from Exosomes Predict Embryonic Development
Authors: Yu Li , Liu Miao , Xu Shiji , Wang Zhenxin , Liu Te , Zhou Jiaye , Zhang Doudou , Dong Xi , Pan Baishen , Wang Beili , Liu Suying , Guo Wei 
Published: March 2022

Abstract
PurposeHuman follicular fluid (FF) is a complex biological fluid that contributes to the microenvironment of oocyte development. The aim of this study was to evaluate the role of steroid levels and mitochonrial function in embryo development during in vitro fertilization cycles.MethodsThis was a cohort study of 138 women receiving IVF/ICSI, including 136 FF samples from 109 infertile women. FF steroid levels were tested by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoassays. The mRNA expression levels of mitochondrial electron transport chain (ETC) complex genes from FF exosomes were detected by qPCR.ResultsAnalysis of these individual FF concentrations revealed that LH and FSH concentrations were higher in follicles in which the oocyte developed into a top quality (TQ) blastocyst (LH: 9.44±2.32mIU/ml, FSH: 9.32±1.01mIU/ml) than those in which there was a failure of fertilization (LH: 5.30±0.84mIU/ml, FSH: 6.91±0.62mIU/ml). In contrast, follicular cortisone concentrations were the lower for oocytes that resulted in a TQ blastocyst (12.20±0.82mIU/ml). The receiver operating characteristic analysis showed that FF LH and FSH levels predicted TQ blastocyst with excellent AUC value of 0.711 and 0.747. Mitochondrial ETC complex I and III mRNA levels were increased in the FF exosomes of TQ blastocyst. Correlation analysis showed that mRNA levels of ETC complex I was positively correlated with LH and FSH levels in FF.ConclusionThe levels of FF steroids from single follicle can predetermine subsequent embryo development to some extent. Furthermore, impaired exosome mitochondrial dysfunction is a potiential event that causes hormone change in embryo development.
Examination of The Relationship Between Tendencies of Narcissism and Eating Disorders in Elite Athletes
Authors: Korkmaz Nimet Haşıl , Ozturk Inci Ece , Mutlu Selen Ugur , Akbas Gamze , Hasturk Meltem 
Published: March 2022

Abstract
Purpose The increase of eating disorders in the athlete population is remarkable. Disorders concerning eating impulse, bodyweight obsession, negative thoughts about body shape, and accompanying affective disorders are psychological disorders in which eating behavior is seriously affected. A narcissistic personality disorder is defined as a continuous pattern that includes superiority complex, inability to empathize, and the need to be approved. Some narcissistic characteristics such as a desire for unlimited power, success, beauty and to be approved may be related to the eating habits of athletes. Therefore, the aim of this study was to examine the relationship between the tendency of narcissism and eating disorders in elite athletes.Methods Participants of the study were 223 elite athletes (99 men, 124 women) from different sports branches aged between 18-36 years old. The Narcissistic Personality Inventory-16 and the Eating Attitude Test were used as data collection tools. Spearman Correlation Analysis and Mann Whitney U Test were used in the analysis of the data. Results A very weak positive relationship was found between eating disorder scores and narcissism scores (p<.05). A very weak positive correlation was found between the exhibitionism sub-dimension and eating disorders tendency (p<.05). Conclusions There is a very weak positive relationship between narcissism tendency and eating disorder tendency variables and also between exhibitionism and eating disorders tendency. Therefore, athletes should be informed about nutrition for their performance by following interdisciplinary approaches.
Modified Conditioning Regimen MCE in Upfront HSCT Provides a Substantial Survival Benefit in High-risk DLBCL
Authors: Ma Jiexian , Sun Shunrong , Hu Yingwei , Wu Min , Shen Lin , Fulati Wulipan , Huang Zilan , Qian Wensi , Chen Pingping , Chen Mingyue , Xie Yanhui 
Published: March 2022

Abstract
Background: High-dose chemotherapy followed by autologous hematopoietic stem cell transplantation (ASCT) remains controversial as a front-line therapy for high-risk diffuse large B cell lymphoma (DLBCL). Moreover, whether modifying the conditioning regimen with anthracyclines for DLBCL will improve the effect and achieve a deeper response in upfront HSCT remains unexplored. Methods: In the present study, we retrospectively compared the outcomes of 156 high-risk DLBCL patients treated with non-HSCT, upfront HSCT or salvage HSCT with a conditioning regimen consisting of mitoxantrone, etoposide, and cyclophosphamide (MCE). Result: We found that an MCE conditioning regimen achieved a complete hematopoietic engraftment and well-tolerated, the incidence of grade 1–2 cardiac toxicity was 3.1%. Transplant-related mortality did not occur. The overall survival (OS) and progression-free survival (PFS) of the upfront group (117.8 and 92.9 months) were substantially longer than that of the non-HSCT group (78.1 and 48.9 months) (P = 0.001 vs P = 0.009), also much longer than that of salvage HSCT group (58.3 m and 21.5 m) (P = 0.00 vs P = 0.00). The multivariate analysis that AnnArbor Stage was related to PFS and OS in upfront HSCT with the MCE regimen. Conclusions: The upfront HSCT with MCE regimen was well-tolerated and exhibited intriguing data in front line therapy to improve the high-risk DLBCL prognosis. However, whether high-risk DLBCL should receive consolidation HSCT with an MCE regimen after an initial CR requires a prospective, large-scale, long-term clinical trials to validate.Retrospectively registered: This study was approved by the Ethical Committee of Huadong Hospital with the Ethical number of 2021K126.
Transfection of Heat Shock Protein 70kDa (HSP70)
Authors: Gestin Maxime , Falato Luca , Ciccarelli Michela , Cerrato Carmine Pasquale , Andréasson Claes , Langel Ülo 
Published: March 2022

Abstract
Heat shock protein 70kDa (HSP70) is a major protein family in the cell protections against stress-induced denaturation and aggregation and in the folding of nascent proteins. It is a highly conserved protein that can be found in most organisms and is strongly connected to several intracellular pathways such as protein folding and refolding, protein degradation and regulation, and protection against intense stress. Cellular delivery of HSP70 would be of high impact for clarification of its role in these cellular processes.PepFect14 is a cell-penetrating peptide known to be able to mediate the transfection of various oligonucleotides to multiple cell lines with a higher efficacy than most commercially available transfection agents and without inducing significant toxic effects.In this study we demonstrated that PepFect14 was able to form a complex with HSP70 and to deliver it inside cells in the same fashion with oligonucleotide delivery. The delivered HSP70 showed an effect in the cell regulation indicating that the protein was biologically available in the cytoplasm and the interactions with PepFect14 did not impeach its active sites once the plasma barrier crossed.This study reports the first successful delivery of HSP70 to our knowledge and the first protein transfection mediated by PepFect14. It opens new fields of research for both PepFect14 as a delivery agent and HSP70 as a therapeutic agent; with potential in peptide aggregation caused diseases such as Parkinson’s and Alzheimer’s diseases.
Functional Responses of Dermal Fibroblasts to Low Nutrition and Pro-Inflammatory Stimuli Mimicking a Wound Environment in Vitro
Authors: Zavadakova Anna , Vistejnova Lucie , Tonarova Pavla 
Published: March 2022

Abstract
Dermal fibroblasts (DF) constitute one of key cells involved in wound healing. However, the functions they perform in wound conditions remain poorly understood. This study involved exposing DF to low nutrition and to low nutrition + LPS for 5 days as conditions representing the wound. Although DF exhibited increasing metabolic activity in time under all conditions including control, the proliferation did not change in both low nutrition and low nutrition + LPS. Only the low nutrition + LPS was found to potentiate the migration and pro-inflammatory phenotype (IL6 release) of DF. The potential of DF to contract collagen hydrogel declined only under the low nutrition as a consequence of low cell number. The expression of α-SMA was reduced under the both conditions independently of the cell number. The remodeling capability of DF was affected under the both conditions as documented by the enhanced MMP2 activity. Finally, the production of collagen type I was not affected by either condition. The study shows that low nutrition as the single factor is able to delay the healing process. Moreover, the addition of the mild pro-inflammatory stimulus represented by LPS may amplify the cell response in case of decreased α-SMA expression or excite DF to produce IL6 impairing the healing process.