bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural tissue engineering: From bioactive scaffolds and in situ monitoring to regeneration
Authors: Gong Bowen , Zhang Xindan , Zahrani Ahmed Al , Gao Wenwen , Ma Guolin , Zhang Liqun , Xue Jiajia 
Published: April 2022

AbstractPeripheral nerve injury is a large‐scale problem that annually affects more than several millions of people all over the world. It remains a great challenge to effectively repair nerve defects. Tissue engineered nerve guidance conduits (NGCs) provide a promising platform for peripheral nerve repair through the integration of bioactive scaffolds, biological effectors, and cellular components. Herein, we firstly describe the pathogenesis of peripheral nerve injuries at different orders of severity to clarify their microenvironments and discuss the clinical treatment methods and challenges. Then, we discuss the recent progress on the design and construction of NGCs in combination with biological effectors and cellular components for nerve repair. Afterward, we give perspectives on imaging the nerve and/or the conduit to allow for the in situ monitoring of the nerve regeneration process. We also cover the applications of different postoperative intervention treatments, such as electric field, magnetic field, light, and ultrasound, to the well‐designed conduit and/or the nerve for improving the repair efficacy. Finally, we explore the prospects of multifunctional platforms to promote the repair of peripheral nerve injury.
Interfacial wettability and mass transfer characterizations for gas–liquid–solid triple‐phase catalysis
Authors: Shi Run , Shang Lu , Zhou Chao , Zhao Yunxuan , Zhang Tierui 
Published: April 2022

AbstractHeterogeneous catalysis is inseparable from interfacial mass transfer and chemical reaction processes determined by the structure and microenvironment. Different from high‐temperature thermochemical processes, photo‐ and electrocatalysis operated at mild conditions often involve both gas and liquid phases, making it important but challenging to characterize the reaction process typically occurring at the gas–liquid–solid interface. Herein, we review the scope, feasibility, and limitation of ten types of currently available technologies used to characterize interfacial wettability and mass transfer properties of various triple‐phase catalytic reactions. The review summarizes techniques from macroscopic contact angle measurement to microscopic environment electron microscopy for investigating the wettability‐controlled structure of triple‐phase interfaces. Experimental and computational methods in revealing the interfacial mass transfer process have also been systematically discussed, followed by a perspective on the opportunities and challenges of advanced characterization methods to help understand the fundamental reaction mechanism of triple‐phase catalysis.
Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics
Authors: Yang Jing , Huang Lin , Qian Kun 
Published: April 2022

AbstractIn vitro diagnostics (IVD) has played an indispensable role in healthcare system by providing necessary information to indicate disease condition and guide therapeutic decision. Metabolic analysis can be the primary choice to facilitate the IVD since it characterizes the downstream metabolites and offers real‐time feedback of the human body. Nanomaterials with well‐designed composition and nanostructure have been developed for the construction of high‐performance detection platforms toward metabolic analysis. Herein, we summarize the recent progress of nanomaterials‐assisted metabolic analysis and the related applications in IVD. We first introduce the important role that nanomaterials play in metabolic analysis when coupled with different detection platforms, including electrochemical sensors, optical spectrometry, and mass spectrometry. We further highlight the nanomaterials‐assisted metabolic analysis toward IVD applications, from the perspectives of both the targeted biomarker quantitation and untargeted fingerprint extraction. This review provides fundamental insights into the function of nanomaterials in metabolic analysis, thus facilitating the design of next‐generation diagnostic devices in clinical practice.
Antimicrobial activity and safety features assessment of Weissella spp. from environmental sources
Authors: Fhoula Imene , Boumaiza Mohamed , Tayh Ghassan , Rehaiem Amel , Klibi Naouel , Ouzari Imene‐Hadda 
Published: April 2022

AbstractWeissella strains have been reported to be useful in biotechnological and probiotic determinations, and some of them are considered opportunistic pathogens. Given the widespread interest about antimicrobial susceptibilities, transmission of resistances, and virulence factors, there is little research available on such topics for Weissella. The aim of this study was to assess the safety aspects and antimicrobial potential of 54 Weissella spp. strains from different environmental sources. Antibiotic susceptibility, hemolytic activity, horizontal transfer, and antibacterial activity were studied, as well as the detection of biogenic amine BA production on decarboxylase medium and PCR was performed. All the strains were nonhemolytic and sensitive to chloramphenicol and ampicillin. Several strains were classified as resistant to fusidic acid, and very low resistance rates were detected to ciprofloxacin, tetracycline, streptomycin, lincomycin, erythromycin, and rifampicin, although all strains had intrinsic resistance to vancomycin, nalidixic acid, kanamycin, and teicoplanin. Two BA‐producing strains (W. halotolerans FAS30 and FAS29) exhibited tyrosine decarboxylase activity, and just one W. confusa FS077 produced both tyramine and histamine, and their genetic determinants were identified. Ornithine decarboxylase/odc gene was found in 16 of the Weissella strains, although 3 of them synthesize putrescine. Interestingly, eight strains with good properties displayed antibacterial activity. Conjugation frequencies of erythromycin from Bacillus to Weissella spp. varied in the average of 3 × 10−9 transconjugants/recipient. However, no tetracycline‐resistant transconjugant was obtained with Enterococcus faecalis JH2‐2 as recipient. The obtained results support the safe status of Weissella strains, derived from environmental sources, when used as probiotics in animal feed.
Testosterone and cortisol responses to ß‐hydroxy ß‐methylbutryate consumption and exercise: A meta‐analysis
Authors: Zhao Li , Mohammad Mohamad 
Published: April 2022

AbstractBackgroundβ‐hydroxy β‐methylbutryate (HMB) is a metabolite of leucine amino acid and it has several ergogenic benefits. Previous studies also showed that it may affect beneficially the testosterone and cortisol concentration in athletes. Due to the contradiction results between studies, we aimed to conduct this meta‐analysis to assess the HMB supplementation effect on testosterone and cortisol in trained athletes.MethodsScopus, Medline, and Google scholar were systematically searched up to August 2021. The Cochrane Collaboration tool for evaluating the risk of bias was applied for assessing the studies' quality. Random‐effects model, weighted mean difference (WMD), and 95% confidence interval (CI) were used for estimating the overall effect. Between‐study heterogeneity was evaluated applying the chi‐squared and I2 statistic.ResultsSeven articles were included in the meta‐analysis. Although the meta‐analysis generally showed that HMB consumption did not have any effect on the cortisol and testosterone concentration (p > .05), subgroup analysis based on the exercise type showed a significant decrease in the cortisol concentration in resistance training exercises (WMD = −3.30; 95% CI: −5.50, −1.10; p = .003) and a significant increase in the testosterone concentration in aerobic and anaerobic combined sports (WMD = 1.56; 95% CI: 0.07, 3.05; p = .040).ConclusionThe results indicate that HMB supplementation in athletes can reduce the concentration of cortisol in resistance exercises and increase the concentration of testosterone in aerobic and anaerobic combined exercises. Nevertheless, more studies are required to confirm these results.
Petrogenesis and tectonic implications of Early Cretaceous granite porphyry in the Taipingtun area, central Great Xing'an Range, NE China
Authors: Tai Baoqiang , Mi Wentian , Wang Genhou , Kong Xu , Li Yingjie , Cheng Jianbo 
Published: April 2022

New LA‐ICP‐MS zircon U–Pb geochronology, whole‐rock geochemistry and Hf isotopic data have been studied on the Taipingtun granite porphyry to determine its petrogenesis and constrain the tectonic setting of the central Great Xing'an Range, northeastern China, during the Early Cretaceous. The results of zircon U–Pb dating suggest that the Taipingtun granite porphyry was emplaced during the Early Cretaceous (122–123 Ma). Geochemically, the granite porphyries have high contents of SiO2 (75.76–78.66 wt%) and (K2O + Na2O) (7.14–8.29 wt%), and low Al2O3 (11.09–12.81 wt%) and CaO (0.15–0.29 wt%), indicating peraluminous and high‐K calc‐alkaline features. Additionally, these rocks are characterized by strong Th, U, K, and Rb enrichment; Ba, Sr, P and Ti depletion; high Ga/Al ratios (10,000*Ga/Al = 2.60–4.92) and negative Eu anomalies (δEu = 0.02–0.13). According to the zircon saturation thermometer results, zircon saturation temperatures range from 756 to 959°C with an average of 823°C, displaying high magmatic temperature for the parental magma. Based on these geochemical characteristics, it can be reasonably inferred that the initial composition of the Taipingtun granite porphyry melts exhibited an A‐type magmatic affinity. Furthermore, zircon grains within the Taipingtun granite porphyry have εHf(t) values of 7.12 to 10.99 and TDM2 model ages of 476–726 Ma. The Ba/La (average 3.89), Nd/Th (average 2.25), Zr/Hf (average 22.55), Ti/Zr (average 2.96) and Rb/Sr (average 4.21) values display the characteristics of crust‐derived magmas. Moreover, these rocks have obviously negative Eu anomalies (average 0.13) and relatively high Y (average 37.83 ppm) and Yb (average 4.15 ppm) contents, suggesting the magmas are generated in a low‐pressure environment. Thus, we consider the primary magmas of the Taipingtun granite porphyry may have been predominantly derived from the partial melting of juvenile crustal rocks at low pressure. Combined with the regional geological data, we suggest that the Taipingtun granite porphyry was formed in an extensional tectonic environment, and possibly related to the subducted Palaeo‐Pacific Ocean Plate in the Early Cretaceous.
Infant brain imaging using magnetoencephalography: Challenges, solutions, and best practices
Authors: Clarke Maggie D. , Bosseler Alexis N. , Mizrahi Julia C. , Peterson Erica R. , Larson Eric , Meltzoff Andrew N. , Kuhl Patricia K. , Taulu Samu 
Published: April 2022

AbstractThe excellent temporal resolution and advanced spatial resolution of magnetoencephalography (MEG) makes it an excellent tool to study the neural dynamics underlying cognitive processes in the developing brain. Nonetheless, a number of challenges exist when using MEG to image infant populations. There is a persistent belief that collecting MEG data with infants presents a number of limitations and challenges that are difficult to overcome. Due to this notion, many researchers either avoid conducting infant MEG research or believe that, in order to collect high‐quality data, they must impose limiting restrictions on the infant or the experimental paradigm. In this article, we discuss the various challenges unique to imaging awake infants and young children with MEG, and share general best‐practice guidelines and recommendations for data collection, acquisition, preprocessing, and analysis. The current article is focused on methodology that allows investigators to test the sensory, perceptual, and cognitive capacities of awake and moving infants. We believe that such methodology opens the pathway for using MEG to provide mechanistic explanations for the complex behavior observed in awake, sentient, and dynamically interacting infants, thus addressing core topics in developmental cognitive neuroscience.
Associations of white matter hyperintensities with networks of gray matter blood flow and volume in midlife adults: A coronary artery risk development in young adults magnetic resonance imaging substudy
Authors: Kim William S. H. , Luciw Nicholas J. , Atwi Sarah , Shirzadi Zahra , Dolui Sudipto , Detre John A. , Nasrallah Ilya M. , Swardfager Walter , Bryan Robert Nick , Launer Lenore J. , MacIntosh Bradley J. 
Published: April 2022

AbstractWhite matter hyperintensities (WMHs) are emblematic of cerebral small vessel disease, yet effects on the brain have not been well characterized at midlife. Here, we investigated whether WMH volume is associated with brain network alterations in midlife adults. Two hundred and fifty‐four participants from the Coronary Artery Risk Development in Young Adults study were selected and stratified by WMH burden into Lo‐WMH (mean age = 50 ± 3.5 years) and Hi‐WMH (mean age = 51 ± 3.7 years) groups of equal size. We constructed group‐level covariance networks based on cerebral blood flow (CBF) and gray matter volume (GMV) maps across 74 gray matter regions. Through consensus clustering, we found that both CBF and GMV covariance networks partitioned into modules that were largely consistent between groups. Next, CBF and GMV covariance network topologies were compared between Lo‐ and Hi‐WMH groups at global (clustering coefficient, characteristic path length, global efficiency) and regional (degree, betweenness centrality, local efficiency) levels. At the global level, there were no between‐group differences in either CBF or GMV covariance networks. In contrast, we found between‐group differences in the regional degree, betweenness centrality, and local efficiency of several brain regions in both CBF and GMV covariance networks. Overall, CBF and GMV covariance analyses provide evidence that WMH‐related network alterations are present at midlife.
Identification of occult metastatic disease via lymphoscintigraphy‐guided neck dissection in N0 oral squamous cell carcinoma
Authors: Earnest‐Noble Lisa B. , Lipsky Rachel S. , Kuhel William I. , Kutler David I. 
Published: April 2022

AbstractBackgroundThe purpose of this study is to assess the efficacy of lymphoscintigraphy‐guided neck dissection (LSG‐ND) in the treatment of N0 oral squamous cell carcinoma.MethodsA retrospective cohort study of patients with N0 oral squamous cell carcinoma (SCC) who had either LSG‐ND or neck dissection (ND) at our institution between 2008 and 2020.ResultsEighty‐seven patients met criteria with N0 oral squamous cell carcinoma with no previous treatment or neck surgery (27 LSG‐ND, 60 ND). Sentinel lymph nodes were identified on the contralateral side in 14.8% of patients with unilateral tumors in the LSG‐ND group, with 22.2% of cases with unexpected lymphatic drainage outside ipsilateral levels I‐III. No recurrences to date have occurred in the LSG‐ND cohort, while 13.3% of patients with ND had regional recurrence (p = 0.04).ConclusionsLSG‐ND provides a greater ability to identify occult metastatic disease with a significant reduction in regional recurrence in N0 oral SCC.
Tripartite motif‐containing protein 31 confers protection against nonalcoholic steatohepatitis by deactivating mitogen‐activated protein kinase kinase kinase 7
Authors: Xu Min‐Xuan , Tan Jun , Ge Chen‐Xu , Dong Wei , Zhang Li‐Ting , Zhu Lian‐Cai , Zhao Jun‐Jie , Wang Long‐Yan , Liu Jin , Wei Hao , Sun Yan , Dai Xian‐Ling , Kuang Qin , Li Yan‐Liang , Li Han , Liu Jun‐Yan , Zou Lei , Liang Ran‐Ran , Zhang Chu‐Feng , Xu Juan , Wang Bo‐Chu 
Published: April 2022

Background & Aims:
As a global health threat, NASH has been confirmed to be a chronic progressive liver disease that is strongly associated with obesity. However, no approved drugs or efficient therapeutic strategies are valid, mainly because its complicated pathological processes is underestimated.

Approach & Results:
We identified the RING‐type E3 ubiquitin transferase‐tripartite motif‐containing protein 31 (TRIM31), a member of the E3 ubiquitin ligases family, as an efficient endogenous inhibitor of transforming growth factor‐beta‐activated kinase 1 (mitogen‐activated protein kinase kinase kinase 7; MAP3K7), and we further confirmed that TRIM31 is an MAP3K7‐interacting protein and promotes MAP3K7 degradation by enhancing ubiquitination of K48 linkage in hepatocytes. Hepatocyte‐specific Trim31 deletion blocks hepatic metabolism homeostasis, concomitant with glucose metabolic syndrome, lipid accumulation, up‐regulated inflammation, and dramatically facilitates NASH progression. Inversely, transgenic overexpression, lentivirus, or adeno‐associated virus‐mediated Trim31 gene therapy restrain NASH in three dietary mice models. Mechanistically, in response to metabolic insults, TRIM31 interacts with MAP3K7 and conjugates K48‐linked ubiquitination chains to promote MAP3K7 degradation, thus blocking MAP3K7 abundance and its downstream signaling cascade activation in hepatocytes.

Conclusions:
TRIM31 may serve as a promising therapeutic target for NASH treatment and associated metabolic disorders.
Distinct responsiveness to rifaximin in patients with hepatic encephalopathy depends on functional gut microbial species
Authors: Yukawa‐Muto Yoshimi , Kamiya Tomonori , Fujii Hideki , Mori Hiroshi , Toyoda Atsushi , Sato Ikuya , Konishi Yusuke , Hirayama Akiyoshi , Hara Eiji , Fukuda Shinji , Kawada Norifumi , Ohtani Naoko 
Published: April 2022

Abstract
Hepatic encephalopathy (HE) is the neuropsychiatric complication of liver cirrhosis (LC). The influence of gut microbiota on HE pathogenesis has been suggested but not precisely elucidated. Here, we investigate how the gut microbial profile changed in patients with HE to clarify the functional gut microbial species associated with HE. We focused on their responses to rifaximin (RFX), a nonabsorbable antibiotic used in HE therapy. Feces samples were collected from patients with decompensated LC (all HE), patients with compensated LC, and healthy controls, and fecal gut microbial profiles were compared using 16S ribosomal RNA gene amplicon and metagenomic sequencing. The linear discriminant analysis effect size was used to identify specific species. Urease‐positive Streptococcus salivarius, which can produce ammonia, was identified as the most significantly abundant gut microbiota in the HE group, and its ability to elevate the levels of blood ammonia as well as brain glutamine was experimentally verified in mice. Urease‐negative Ruminococcus gnavus was also identified as a significantly abundant species in patients with RFX‐nonresponsive HE after RFX administration. Interestingly, R. gnavus enhanced urease activity of recombinant urease itself, implying that R. gnavus could amplify ammonia production of surrounding urease‐positive microbiota. Furthermore, the sensitivity of S. salivarius and R. gnavus to RFX depended on conjugated secondary bile acid levels, suggesting a therapeutic potential of the combined use of secondary bile acid levels with RFX for enhancing the efficacy of RFX. This study identified specific gut bacterial species abundant in patients with HE and verified their functions linked to HE pathophysiology. Targeting these bacteria could be a potentially effective strategy to treat HE.
Disentangling prescribing behaviour of Cypriot physicians, within a complex framework of interacting
Authors: Theodorou Mamas , Kontemeniotis Antonis , Kantaris Marios , Farmakas Antonis 
Published: April 2022

AbstractThe purpose of the study is to investigate how physicians' prescribing behaviour in Cyprus adopts to the fragmented healthcare system and to the inadequacies of pharmaceutical market in times of economic crisis. A survey was carried out in using a postal questionnaire administered to a stratified sample of 320 physicians. The questionnaire used was the same with the one used in 2007 survey carried out in Greece and Cyprus, along with complementary questions for prescribing within economic crisis. The comparative analysis and assessment of the findings from the two surveys revealed that the current system and the inadequacies of pharmaceutical market in Cyprus expose physicians to a contrasting environment of public and private sector in terms of incentives, governance principles, financing and market structure. In contrast to public sector prescribers who have behaved in accordance with the governance principles, there is a strong motivation for private sector physicians to favour new branded products, and generally rejecting any ideas that could limit their clinical autonomy. Economic crisis seems to be unilaterally influential, as public sector physicians became more cost conscious while private sector prescribing is still resisting due to strong financial incentives.
Long‐term incidence in hepatocellular carcinoma and intrahepatic bile duct cancer in Denmark, Finland, Norway and Sweden, role of Thorotrast?
Authors: Hemminki Kari , Tichanek Filip , Försti Asta , Hemminki Otto , Liska Vaclav , Hemminki Akseli 
Published: April 2022

AbstractWe analyzed long‐term incidence trends in liver cancer (including hepatocellular carcinoma and intrahepatic cholangiocarcinoma) with an aim to interpret the changes in terms of known risk factors and hypothesize that historical exposure to Thorotrast, a radiographic contrast medium emitting alpha particles, has changed population rates. The NORDCAN database was used to collect cancer registry data from Denmark (DK), Finland (FI), Norway (NO) and Sweden (SE), which we used from 1953 (DK, FI and NO) and 1960 (SE) through 2019. Thorotrast, which caused a 100‐fold risk of liver cancer was used in DK and SE, and probably also in FI between 1930 and 1950, but not in NO. The incidence trend for liver cancer showed a broad maximum at around 1980, most prominent and statistically significant in SE and DK men and women, and in all countries, a steadily increasing trend towards the end of follow‐up. Incidence for NO was lower than for the other countries and the rates showed no peaking at around 1980. Birth cohort analysis identified a transient risk which could be dated to a period between 1930 and 1950 in countries other than NO. Considering a lag time between Thorotrast use and liver cancer appearance, the large incidence peak around 1980 in DK and DE was probably contributed by Thorotrast but considering the ecological nature of the findings, the association should be considered cautiously as hypothesis generating. The late increase in liver cancer risk is most likely lifestyle related and largely preventable.