bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soybean is a Common Nectar Source for Honey Bees (Hymenoptera: Apidae) in a Midwestern Agricultural Landscape
Authors: Lin Chia-Hua , Suresh Sreelakshmi , Matcham Emma , Monagan Paityn , Curtis Hailey , Richardson Rodney T , Johnson Reed M 
Published: September 2022

Abstract
Large-scale soybean [Glycine max (L.) Merr.] cultivation has substantially transformed the Midwestern landscape in recent decades. Floral nectar produced by immense fields of soybeans has the potential to influence foraging ecology and resource accumulation of honey bee (Apis mellifera L.) colonies. In this study, we combined microscopic and molecular pollen analysis of honey samples with waggle dance inference of spatial foraging patterns to demonstrate that honey bees routinely forage on soybeans in Ohio. In analyzing honey samples from across the state, we found ubiquitous presence of soybean pollen in honey collected from agricultural lands during soybean bloom. The abundance of soybean pollen in honey increased with the amount of soybean fields surrounding the apiaries. Honey bee waggle dances recorded during soybean bloom revealed that honey bees preferred soybean fields for foraging over other habitat types. With these results, future research efforts aimed at enhancing mutual interactions between soybeans and honey bees may represent an unexplored pathway for increasing soybean production while supporting honey bees and other pollinators in the surrounding landscape.
Corn Stunt Pathosystem and Its Leafhopper Vector in Brazil
Authors: Pozebon Henrique , Stürmer Glauber Renato , Arnemann Jonas André 
Published: September 2022

AbstractDirect and indirect injury caused by Dalbulus maidis (Hemiptera: Cicadellidae) in corn is an ever-increasing concern in Brazil and other corn-producing countries of the Americas. This highly efficient vector transmits corn stunting pathogens and is of economic concern in the Neotropics, including temperate regions where epidemic outbreaks are now common. Despite the progress made so far, Brazilian corn growers continue to struggle with this pest and its associated pathosystem. In this review, we gathered relevant and updated information on the bioecology, population dynamics, and damaging potential of D. maidis. Our goal was to better understand its intimate association and complex interactions with the host crop and transmitted pathogens. Based on available scientific literature, we identified factors which explain the recent increase in D. maidis occurrence in South America, including the cultivation of corn during multiple growing seasons, overlapping of susceptible crops, and widespread use of genetically modified hybrids. The reasons for the overall inefficiency of current suppression strategies aimed at this pest are also summarized. Finally, a management program for D. maidis and corn stunt disease is proposed, combining strategies such as eradicating volunteer corn, reducing the planting period, using tolerant hybrids, and applying chemical and/or fungal insecticides. Prospects regarding the pest’s status are also outlined. Overall, the information presented here will serve as a decision-making guide within Brazilian and South American corn production systems, as well as paving the way for devising novel strategies aimed at suppressing D. maidis populations and limiting the spread of corn stunt disease.
Distribution and Ecology of Phlebotomine Sand Flies (Diptera: Psychodidae) in Endemic and Nonendemic Area of Leishmaniasis in Northern Morocco
Authors: Zarrouk Asmae , Boussaa Samia , Belqat Boutaïna 
Published: September 2022

Abstract
Northern Morocco is endemic for cutaneous and visceral leishmaniasis. Our entomological investigations aim to evaluate the risk of Leishmania transmission by determining the species composition, the density, and seasonal fluctuation of sand fly populations in endemic and nonendemic areas of leishmaniasis in Tetouan province (North-Western Morocco). Using Sticky-paper traps, 8,370 specimens were collected between May and November 2015 in two localities: peri-urban area of Tetouan city, where leishmaniasis is endemic and that of the Oued Laou village where no cases of leishmaniasis have been recorded. Six sand fly species were identified. The genus Phebotomus was represented by five species: Phlebotomus ariasi, Phlebotomus. longicuspis, Phlebotomus perniciosus, Phlebotomus kazeruni, and Phlebotomus sergenti, while the genus Sergentomyia was represented by only one species Sergentomyia minuta. Phlebotomus perniciosus was dominant in the nonendemic area (47%) while Phlebotomus sergenti was dominant in the endemic area (51%). The spatio-temporal distribution of sand fly populations is discussed according to biotic and abiotic variables. Seasonal fluctuation in sand fly density showed a bimodal pattern for the subgenus Larroussius and a unimodal pattern for the subgenus Paraphlebotomus in Tetouan city. But, in Oued Laou village, a unimodal density distribution for species of the Larroussius subgenus and a bimodal seasonal distribution for species of the subgenus Paraphlebotomus were identified. We affirm the coexistence, in the study area, of vectors of both cutaneous and visceral leishmaniasis, namely P. ariasi, P. longicuspis and P. perniciosus vectors of Leishmania infantum and P. sergenti vector of L. tropica. However, the geographic distribution, the specific abundance, and the activity reveal significant differences between endemic and nonendemic areas in the region.
Phoenixin 14 ameloriates pancreatic injury in streptozotocin-induced diabetic rats by alleviating oxidative burden
Authors: Ozdemir-Kumral Zarife Nigâr , Sen Eminenur , Yapici Hasan Basri , Atakul Nurullah , Domruk Omer Faruk , Aldag Yusra , Sen Leyla Semiha , Kanpalta Mustafaoğlu Fatma , Yuksel Meral , Akakin Dilek , Erzik Can , Haklar Goncagul , imeryuz Neşe 
Published: September 2022

Abstract
Phoenixin-14 (PNX) is a neuropeptide that has been shown to prevent oxidative damage and stimulates insulin secretion. We investigated the effects of PNX on pancreatic injury induced by streptozotocin (STZ), and nicotinamide (NAD). Male Sprague-Dawley rats, in control (C) and diabetic (STZ) groups, were treated with either saline, or PNX (0.45 nmol/kg, or 45 nmol/kg) daily for 3 days 1 week after STZ injection. Fasting blood glucose (FBG) and gastric emptying rate (GER) were measured. Tissue and blood samples were collected. PNX treatments prevented pancreatic damage and β cell loss. Increased luminol and lucigenin levels in the pancreas, ileum and liver tissues of STZ groups were alleviated by PNX treatment in pancreatic and ileal tissues. PNX0.45 decreased FBG without any change in insulin blood level and pancreatic mRNA. GER increased in all diabetic rats while PNX0.45 delayed GER only in the C group. PNX diminishes pancreatic damage and lowers FBG by reducing oxidative load.
High quality mapping of chromatin at or near the nuclear lamina from small numbers of cells reveals cell cycle and developmental changes of chromatin at the nuclear periphery
Authors: Tran Joseph R , Zheng Xiaobin , Adam Stephen A , Goldman Robert D , Zheng Yixian 
Published: September 2022

AbstractThe chromatin associated with the nuclear lamina (NL) is referred to as lamina-associated domains (LADs). Here, we present an adaptation of the tyramide-signal amplification sequencing (TSA-seq) protocol, which we call chromatin pull down-based TSA-seq (cTSA-seq), that can be used to map chromatin regions at or near the NL from as little as 50 000 cells. The cTSA-seq mapped regions are composed of previously defined LADs and smaller chromatin regions that fall within the Hi-C defined B-compartment containing nuclear peripheral heterochromatin. We used cTSA-seq to map chromatin at or near the assembling NL in cultured cells progressing through early G1. cTSA-seq revealed that the distal ends of chromosomes are near or at the reassembling NL during early G1, a feature similar to those found in senescent cells. We expand the use of cTSA-seq to the mapping of chromatin at or near the NL from fixed-frozen mouse cerebellar tissue sections. This mapping reveals a general conservation of NL-associated chromatin and identifies global and local changes during cerebellar development. The cTSA-seq method reported here is useful for analyzing chromatin at or near the NL from small numbers of cells derived from both in vitro and in vivo sources.
RiboTaxa: combined approaches for rRNA genes taxonomic resolution down to the species level from metagenomics data revealing novelties
Authors: Chakoory Oshma , Comtet-Marre Sophie , Peyret Pierre 
Published: September 2022

Abstract
Metagenomic classifiers are widely used for the taxonomic profiling of metagenomics data and estimation of taxa relative abundance. Small subunit rRNA genes are a gold standard for phylogenetic resolution of microbiota, although the power of this marker comes down to its use as full-length. We aimed at identifying the tools that can efficiently lead to taxonomic resolution down to the species level. To reach this goal, we benchmarked the performance and accuracy of rRNA-specialized versus general-purpose read mappers, reference-targeted assemblers and taxonomic classifiers. We then compiled the best tools (BBTools, FastQC, SortMeRNA, MetaRib, EMIRGE, VSEARCH, BBMap and QIIME 2’s Sklearn classifier) to build a pipeline called RiboTaxa. Using metagenomics datasets, RiboTaxa gave the best results compared to other tools (i.e. Kraken2, Centrifuge, METAXA2, phyloFlash, SPINGO, BLCA, MEGAN) with precise taxonomic identification and relative abundance description without false positive detection (F-measure of 100% and 83.7% at genus level and species level, respectively). Using real datasets from various environments (i.e. ocean, soil, human gut) and from different approaches (e.g. metagenomics and gene capture by hybridization), RiboTaxa revealed microbial novelties not discerned by current bioinformatics analysis opening new biological perspectives in human and environmental health.
Association between the fatty liver index and chronic kidney disease: the population-based KORA study
Authors: Cai Xinting , Thorand Barbara , Hohenester Simon , Koenig Wolfgang , Rathmann Wolfgang , Peters Annette , Nano Jana 
Published: September 2022

ABSTRACT

Background
We aimed to evaluate the relationship of fatty liver, estimated by the fatty liver index (FLI), with kidney function and chronic kidney disease (CKD) in a German cohort study, given the lack of prospective evidence in Europeans.

Methods
We included 2920 participants (51.6% women, mean age 56.1 years) from the KORA study, of which 1991 were followed up for an average of 6.5 years (± 0.3). Kidney function was assessed using the glomerular filtration rate estimated by creatinine (eGFR-Cr) or cystatin C (eGFR-cC). We used multiple logistic or linear regressions to evaluate the associations between the FLI, kidney function and CKD (eGFR &lt; 60 ml/min/1.73 m2) and mediation analysis to explore the mediation effects of metabolic factors.

Results
The prevalence of FLI ≥60 and CKD was 40.4% and 5.6% at baseline, respectively, and 182 participants developed CKD during the follow-up. Cross-sectionally, FLI was significantly inversely associated with eGFR-cC {β = −1.14 [95% confidence interval (CI) −1.81 to −0.47]} and prevalent CKD based on eGFR-cC [OR 1.28 (95% CI 1.01–1.61)], but not with other markers. After adjusting for lifestyle factors, we found a positive association between FLI and incident CKD defined by eGFR-cC or/eGFR-Cr, which was attenuated after controlling for metabolic risk factors. Mediation analysis showed that the association was completely mediated by inflammation, diabetes and hypertension jointly.

Conclusion
The positive association between FLI and CKD incidence was fully mediated by the joint effect of metabolic risk factors. Future longitudinal studies need to explore the chronological interplay between fatty liver, cardiometabolic risk factors and kidney function with repeated measurements.
The type-B response regulators ARR10, ARR12, and ARR18 specify the central cell in Arabidopsis
Authors: Zhu Mingsong , Tao Liang , Zhang Jinghua , Liu Ruini , Tian Hongai , Hu Chong , Zhu Yafen , Li Meizhen , Wei Zhuoyun , Yi Jing , Li Jia , Gou Xiaoping 
Published: September 2022

Abstract
In Arabidopsis thaliana, the female gametophyte consists of two synergid cells, an egg cell, a diploid central cell, and three antipodal cells. CYTOKININ-INDEPENDENT 1 (CKI1), a histidine kinase constitutively activating the cytokinin signaling pathway, specifies the central cell and restricts the egg cell. However, the mechanism regulating CKI1-dependent central cell specification is largely unknown. Here, we showed that the type-B ARABIDOPSIS RESPONSE REGULATORS10, 12, and 18 (ARR10/12/18) localize at the chalazal pole of the female gametophyte. Phenotypic analysis showed that the arr10 12 18 triple mutant is female sterile. We examined the expression patterns of embryo sac marker genes and found that the embryo sac of arr10 12 18 plants had lost central cell identity, a phenotype similar to that of the Arabidopsis cki1 mutant. Genetic analyses demonstrated that ARR10/12/18, CKI1, and ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN2, 3, and 5 (AHP2/3/5) function in a common pathway to regulate female gametophyte development. In addition, constitutively activated ARR10/12/18 in the cki1 embryo sac partially restored the fertility of cki1. Results of transcriptomic analysis supported the conclusion that ARR10/12/18 and CKI1 function together to regulate the identity of the central cell. Our results demonstrated that ARR10/12/18 function downstream of CKI1–AHP2/3/5 as core factors to determine cell fate of the female gametophyte.
Frequent occurrence of mutations in nsp3 and nsp4 of SARS-CoV-2, presumably caused by the inhaled asthma drug ciclesonide
Authors: Doi Akihiro , Tomita Yuriko , Okura Hiyori , Matsuyama Shutoku 
Published: September 2022

Abstract
Mutations in nonstructural protein 3 (nsp3) and nsp4 of SARS-CoV-2, presumably induced by the asthma drug ciclesonide (which also has anti-SARS-CoV-2 activity), were counted 5,851 cases in the GISAID EpiCoV genome database. Sporadic occurrence of mutants not linked to each other in the phylogenetic tree were identified at least 88 times; of which, 58 had one or more descendants in the same branch. Five of these had spread to more than 100 cases, and one had expanded to 4,748 cases, suggesting the mutations are frequent, selected in individual patients, and transmitted to form clusters of cases. Clinical trials of ciclesonide as a treatment for COVID-19 are the presumed cause of the frequent occurrence of mutations between 2020 June and 2021 November. In addition, because ciclesonide is a common treatment for asthma, it can drive mutations in asthmatics suffering from COVID-19. Ciclesonide-resistant mutations, which have unpredictable effects in humans, are likely to continue to emerge because SARS-CoV-2 remains prevalent globally.
Regenerative cerium oxide nanozymes alleviate oxidative stress for efficient dry eye disease treatment
Authors: Zou Haoyu , Wang Haiting , Xu Baoqi , Liang Lin , Shen Liangliang , Lin Quankui 
Published: September 2022

Abstract
Dry eye disease (DED) is the most common eye disease in ophthalmic consultation except for refractive errors. Therefore, an exploration of valid and alternative therapeutic interventions is essential to feed the urgent medical need. It has been demonstrated that oxidative stress causes multiple adverse effects in the pathogenesis of DED, thence alleviating oxidative stress is an effective therapeutic strategy for the DED treatment. Herein, we developed a cerium oxide nanozyme combined with branched poly(ethylene imine)-graft-poly(ethylene glycol) (bPEI-g-PEG). Owing to its stable hydrophilic chains on the surface reducing the cytotoxicity and loads of amines groups that be combined with cerium ions through coordination bonds, the modified nanozymes (referred to as CNP@bPEI-g-PEG) are water soluble and highly biocompatible. Meanwhile, due to its excellent antioxidant activity, CNP@bPEI-g-PEG nanozymes can mimic the activity of superoxide dismutase and catalase to scavenge intracellular reactive oxygen species (ROS). Experimental studies firmly demonstrated that the modified nanozymes were auto-regenerative and more active in scavenging excessive ROS and alleviating oxidative stress by cerium-element valence state recycling, recovering the morphology of corneal, conjunctival epithelium and the number of goblet cells. The advanced combination may offer a superior therapeutic strategy to deal with oxidative stress for effective treatment of DED.
Geostatistical modelling of the distribution, risk and burden of podoconiosis in Kenya
Authors: Deribe Kebede , Sultani Hadley Matendechero , Okoyo Collins , Omondi Wyckliff P , Ngere Isaac , Newport Melanie J , Cano Jorge 
Published: September 2022

AbstractBackgroundUnderstanding and accurately predicting the environmental limits, population at risk and burden of podoconiosis are critical for delivering targeted and equitable prevention and treatment services, planning control and elimination programs and implementing tailored case finding and surveillance activities.MethodsThis is secondary analysis of a nationwide podoconiosis mapping survey in Kenya. We combined national representative prevalence survey data of podoconiosis with climate and environmental data, overlayed with population figures in a geostatistical modelling framework, to predict the environmental suitability, population living in at-risk areas and number of cases of podoconiosis in Kenya.ResultsIn 2020, the number of people living with podoconiosis in Kenya was estimated to be 9344 (95% uncertainty interval 4222 to 17 962). The distribution of podoconiosis varies by geography and three regions (Eastern, Nyanza and Western) represent &gt;90% of the absolute number of cases. High environmental suitability for podoconiosis was predicted in four regions of Kenya (Coastal, Eastern, Nyanza and Western). In total, 2.2 million people live in at-risk areas and 4.2% of the total landmass of Kenya is environmentally predisposed for podoconiosis.ConclusionsThe burden of podoconiosis is relatively low in Kenya and is mostly restricted to certain small geographical areas. Our results will help guide targeted prevention and treatment approaches through local planning, spatial targeting and tailored surveillance activities.
Melioidosis
Authors: Borton Dorothy 
Published: September 2022

Abstract:
Recent cases suggest that melioidosis, an infection caused by Burkholderia pseudomallei, is an emerging infectious disease. Nurses have a key role in the care of patients with melioidosis. This article provides an overview of the epidemiology, clinical presentation, diagnosis, treatment, and prevention of melioidosis, and discusses unusual, non-travel-related cases of melioidosis.
Implementation of Telemedicine Delivery of Medications for Opioid Use Disorder in Pennsylvania Treatment Programs During COVID-19
Authors: Poulsen Melissa N. , Santoro William , Scotti Roseanne , Henderson Catherine , Ruddy Meaghan , Colistra Angela 
Published: September 2022

Introduction
Temporary policy changes during the coronavirus disease 2019 pandemic facilitated rapid expansion of medication for opioid use disorder via telemedicine (tele-MOUD). Evidence for tele-MOUD best practices and its impact on treatment engagement and retention remains limited. This quality improvement initiative compared tele-MOUD implementation among Pennsylvania medication for opioid use disorder (MOUD) programs, evaluated sociodemographic characteristics of patients using tele-MOUD, and described trends in tele-MOUD use and patient engagement and retention.

Methods
Five health systems with MOUD programs completed questionnaires regarding their tele-MOUD models and provided aggregated sociodemographic data for MOUD patients with in-person and telemedicine visits in 2020. Three programs provided aggregated monthly appointment data (scheduled, completed, no-show, tele-MOUD visits) over the period in which tele-MOUD scaled up.

Results
Differences in tele-MOUD protocols related to provision of tele-MOUD inductions, patient eligibility for tele-MOUD, and operationalization of remote drug testing. Across programs, 88% of prescribers conducted tele-MOUD appointments, and 50% of patients used tele-MOUD in 2020. We observed sociodemographic differences, with a greater proportion of female, White, and non-Hispanic patients using tele-MOUD. Across programs with appointment data, overall patient enrollment increased, and new patient enrollment remained relatively constant. Engagement trends suggested a temporary decline in no-show appointments that aligned with the escalation of tele-MOUD in one program.

Conclusions
Tele-MOUD protocol differences indicate a need for research to inform evidence-based guidance. Findings suggest that patients largely remained engaged and retained in MOUD as tele-MOUD was implemented but reveal inequities in tele-MOUD use, highlighting the need for efforts to overcome technology access barriers and avoid exacerbating disparities in MOUD access.
Risk Stratification Index 3.0, a Broad Set of Models for Predicting Adverse Events during and after Hospital Admission
Authors: Greenwald Scott , Chamoun George F. , Chamoun Nassib G. , Clain David , Hong Zhenyu , Jordan Richard , Manberg Paul J. , Maheshwari Kamal , Sessler Daniel I. 
Published: September 2022

Background
Risk stratification helps guide appropriate clinical care. Our goal was to develop and validate a broad suite of predictive tools based on International Classification of Diseases, Tenth Revision, diagnostic and procedural codes for predicting adverse events and care utilization outcomes for hospitalized patients.

Methods
Endpoints included unplanned hospital admissions, discharge status, excess length of stay, in-hospital and 90-day mortality, acute kidney injury, sepsis, pneumonia, respiratory failure, and a composite of major cardiac complications. Patient demographic and coding history in the year before admission provided features used to predict utilization and adverse events through 90 days after admission. Models were trained and refined on 2017 to 2018 Medicare admissions data using an 80 to 20 learn to test split sample. Models were then prospectively tested on 2019 out-of-sample Medicare admissions. Predictions based on logistic regression were compared with those from five commonly used machine learning methods using a limited dataset.

Results
The 2017 to 2018 development set included 9,085,968 patients who had 18,899,224 inpatient admissions, and there were 5,336,265 patients who had 9,205,835 inpatient admissions in the 2019 validation dataset. Model performance on the validation set had an average area under the curve of 0.76 (range, 0.70 to 0.82). Model calibration was strong with an average R
2 for the 99% of patients at lowest risk of 1.00. Excess length of stay had a root-mean-square error of 0.19 and R
2 of 0.99. The mean sensitivity for the highest 5% risk population was 19.2% (range, 11.6 to 30.1); for positive predictive value, it was 37.2% (14.6 to 87.7); and for lift (enrichment ratio), it was 3.8 (2.3 to 6.1). Predictive accuracies from regression and machine learning techniques were generally similar.

Conclusions
Predictive analytical modeling based on administrative claims history can provide individualized risk profiles at hospital admission that may help guide patient management. Similar results from six different modeling approaches suggest that we have identified both the value and ceiling for predictive information derived from medical claims history.

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