bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simple, sensitive, and cost-effective detection of wAlbB Wolbachia in Aedes mosquitoes, using loop mediated isothermal amplification combined with the electrochemical biosensing method
Authors: Thayanukul Parinda , Lertanantawong Benchaporn , Sirawaraporn Worachart , Charasmongkolcharoen Surat , Chaibun Thanyarat , Jittungdee Rattanalak , Kittayapong Pattamaporn 
Published: May 2022

Background
Wolbachia is an endosymbiont bacterium generally found in about 40% of insects, including mosquitoes, but it is absent in Aedes aegypti which is an important vector of several arboviral diseases. The evidence that Wolbachia trans-infected Ae. aegypti mosquitoes lost their vectorial competence and became less capable of transmitting arboviruses to human hosts highlights the potential of using Wolbachia-based approaches for prevention and control of arboviral diseases. Recently, release of Wolbachia trans-infected Ae. aegypti has been deployed widely in many countries for the control of mosquito-borne viral diseases. Field surveillance and monitoring of Wolbachia presence in released mosquitoes is important for the success of these control programs. So far, a number of studies have reported the development of loop mediated isothermal amplification (LAMP) assays to detect Wolbachia in mosquitoes, but the methods still have some specificity and cost issues.

Methodology/Principal findings
We describe here the development of a LAMP assay combined with the DNA strand displacement-based electrochemical sensor (BIOSENSOR) method to detect wAlbB Wolbachia in trans-infected Ae. aegypti. Our developed LAMP primers used a low-cost dye detecting system and 4 oligo nucleotide primers which can reduce the cost of analysis while the specificity is comparable to the previous methods. The detection capacity of our LAMP technique was 1.4 nM and the detection limit reduced to 2.2 fM when combined with the BIOSENSOR. Our study demonstrates that a BIOSENSOR can also be applied as a stand-alone method for detecting Wolbachia; and it showed high sensitivity when used with the crude DNA extracts of macerated mosquito samples without DNA purification.

Conclusions/Significance
Our results suggest that both LAMP and BIOSENSOR, either used in combination or stand-alone, are robust and sensitive. The methods have good potential for routine detection of Wolbachia in mosquitoes during field surveillance and monitoring of Wolbachia-based release programs, especially in countries with limited resources.
ANALISIS ANTREAN BUS NONPATAS JALUR TIMUR TERMINAL TIRTONADI KOTA SURAKARTA MENGGUNAKAN METODE BAYESIAN
Authors: Faizah Rizka Nur , Sugito Sugito , Sudarno Sudarno 
Published: May 2022

The queuing system relates to customers and service facilities. Queuing theory designs service facilities to address service requests. Queues occur if the service capacity is not sufficient to provide services to many customers. The queuing phenomenon occurs on non-patas buses on the eastern route of Tirtonadi Terminal, Surakarta with Surabaya, Karanganyar, Wonogiri, Purwodadi and Pedesaan buses. The Bayesian method combines information from current research and previous studies with similar cases, and produces a posterior distribution to form a queuing system model and measure of service system performance. The bus queuing system model for Surabaya, Karanganyar, Wonogiri and Purwodadi has a Gamma-distributed arrival and service pattern. Pedesaan buses has an arrival pattern with a Gamma distribution and a service pattern with an Inverse Gamma distribution. Each line has 1 bus line as a service system, FIFO queue discipline, the number of customer capacity and call sources is not limited. The Surabaya buses has the highest probability of 93.49% that the line is idle and the Pedesaan buses has the highest probability that the line will be busy serving at 89.50%. The queuing system are considered good because the five lines of service facilities are able to meet customer needs. Keywords: Tirtonadi Terminal, Bayesian, Posterior Distribution, Queue Models, System Performance Measures
METODE BAYESIAN PADA SISTEM ANTREAN PELAYANAN MENGGUNAKAN GUI R (Studi Kasus: Antrean Pelayanan di Kantor Dinas Kependudukan dan Pencatatan Sipil Kota Semarang)
Authors: Mufidah Atikah , Sugito Sugito , Maruddani Di Asih I 
Published: May 2022

The increase population of Semarang City has given many kinds of problem from births, deaths, marriages and other important events. The change of population identity data causes the number of visitors to the Semarang City Dispendukcapil to increase so that the service system becomes busy. The study aims to determine whether the service system in the Dispendukcapil is good or not. This can be known by determining the distribution of arrival patterns and service patterns to obtain a queuing system model and system performance measures. In this study, the distribution of arrival patterns and service patterns is determined by finding the posterior distribution using the Bayesian method. The Bayesian method was chosen because it is able to combine the distribution of the sample in the current study with previous information for the same case. Posterior distribution can be obtained if it has elements, namely prior distribution and likelihood function. The distribution of arrival patterns and service patterns obtained from prior information, follows the Discrete Uniform and Log-Normal distribution. Based on the calculation and analysis of the posterior distribution, the service system model of the Dispendukcapil Semarang City is obtained, namely for the Customer Service counter, and for the legalization counter and the population document service counter with a good service system.Keywords:Population, Dispendukcapil Semarang City, queue, Bayesian, prior distribution, posterior distribution, queuing system model, Beta, Gamma, Inverse Gamma.
Lump solutions to a generalized nonlinear PDE with four fourth-order terms
Authors: Chen Qingxian , Ma Wen-Xiu , Huang Yehui 
Published: May 2022

Abstract
A combined fourth-order (2 + 1)-dimensional nonlinear partial differential equation which contains four fourth-order nonlinear terms and all second-order linear terms is formulated. This equation covers three generalized KP, Hirota–Satsuma–Ito, and Calogero–Bogoyavlenskii–Schiff equations as examples, which have physical applications in the study of various nonlinear phenomena in nature. In terms of some settings of the coefficients, a class of lump solutions is constructed by the Hirota bilinear method and the solutions are calculated through the symbolic computation system of Maple. Meanwhile, the relation between the coefficients and the solution is explored. Two special lump solutions are generated by taking proper values for the involved coefficients and parameters, and their dynamic behaviors are studied, as illustrative examples. The primary advantage of the Hirota bilinear method is to transform a nonlinear equation into a bilinear one so that the targeted equation can be easily studied.
(Mal-)practices of Auctioneering on the English Property Market During the 19th Century
Authors: Fasold Stephan 
Published: May 2022

Abstract
In the 19th century, auctions became a widespread form of transaction for real estate in England. Contemporaries viewed auctioning as an effective method for the transaction of land, in terms of price determination and transparency. Contrary to these theoretical assumptions, the article shows that in practice it was not economic efficiency but group interests that played a decisive role in the establishment of the auction mechanism. The article provides an analysis of the formal and informal rules of the auction trade for real estate and argues that 19th century real estate auctions did not always lead to optimal results in terms of valuation and allocation because power structures played a greater role in them than auction theory assumes.
Glücksspiel oder ökonomisches Werturteil mit Folgen?
Authors: Schroeter Felix 
Published: May 2022

Abstract
Despite his prominence in the socio-political debate on the German housing market before the First World War, Rudolph Eberstadt’s (1856-1922) concept of speculation is largely ignored today by German scholars of economics and economic history. Accordingly, speculative operations on markets are seen rather to determine economic values than to merely assess them. Through the channel of the prevailing institutions of credit and property, they determine future values as well as the modalities of how the real economy will produce their corresponding equivalents. Eberstadt’s conceptual works on the general notion of speculation rely on his institutional-economic investigation of the housing market in metropolitan areas. He conceives loan-financed speculative deals as a legally backed instrument of redistribution, which serves the private sector to realize capital gains from mere value assessments. His approach critically highlights the normative power of financial liabilities from a politicoeconomic perspective. The severe criticism that this approach evoked on the part of market-liberal economists may explain why academics largely ignore Eberstadt’s insights today.
Value of soluble fms-like tyrosine kinase-1/placental growth factor test in third trimester of pregnancy for predicting preeclampsia in asymptomatic women
Authors: Hanson Ele , Rull Kristiina , Ratnik Kaspar , Vaas Pille , Teesalu Pille , Laan Maris 
Published: May 2022

Abstract

Objectives
To estimate the value of screening maternal serum soluble fms-like tyrosine kinase/placental growth factor (sFlt-1/PlGF) ratio in asymptomatic women during 3rd trimester to predict preeclampsia (PE) development.

Methods
The investigated group comprised of 178 pregnant women. During this gestation, 24 cases had developed PE and 12 isolated gestational hypertension (GH); whereas 142 remained normotensive. Blood samples were collected between 180 and 259 gestational days (g.d.) when the participants were asymptomatic. Serums were analyzed using the BRAHMS sFlt-1 Kryptor/BRAHMS PlGF plus Kryptor PE ratio test (Thermo Fisher Scientific, Henningdorf, Germany). High-risk pregnancies for the PE development were defined as sFlt-1/PlGF>38.

Results
The detection rate (DR) for manifestation of PE≤30 days after sampling was 83.3% and overall DR during pregnancy 58.3%. Ten of 15 women having false positive prediction of PE suffered from GH, preterm birth and/or delivery of a small-for-gestational-age-newborn. False positive rate was significantly higher at 239–253 g.d. compared to sampling at 210–224 g.d. and 225–238 g.d. (21.9% vs. 7.8% and 5.3%; p < 0.05).

Conclusions
The sFlt-1/PlGF test during 180–259 g.d. detected approximately half of subsequent PE cases. An optimal time to use the test for screening purposes was estimated 225–238 g.d. (DR 66.7%). False positive test results were more common to cases with other adverse pregnancy outcomes and samples drawn at higher gestational age.
Photonic (computational) memories: tunable nanophotonics for data storage and computing
Authors: Lian Chuanyu , Vagionas Christos , Alexoudi Theonitsa , Pleros Nikos , Youngblood Nathan , Ríos Carlos 
Published: May 2022

Abstract
The exponential growth of information stored in data centers and computational power required for various data-intensive applications, such as deep learning and AI, call for new strategies to improve or move beyond the traditional von Neumann architecture. Recent achievements in information storage and computation in the optical domain, enabling energy-efficient, fast, and high-bandwidth data processing, show great potential for photonics to overcome the von Neumann bottleneck and reduce the energy wasted to Joule heating. Optically readable memories are fundamental in this process, and while light-based storage has traditionally (and commercially) employed free-space optics, recent developments in photonic integrated circuits (PICs) and optical nano-materials have opened the doors to new opportunities on-chip. Photonic memories have yet to rival their electronic digital counterparts in storage density; however, their inherent analog nature and ultrahigh bandwidth make them ideal for unconventional computing strategies. Here, we review emerging nanophotonic devices that possess memory capabilities by elaborating on their tunable mechanisms and evaluating them in terms of scalability and device performance. Moreover, we discuss the progress on large-scale architectures for photonic memory arrays and optical computing primarily based on memory performance.
ERGONOMICS KNOWLEDGE AMONG ENGINEERING STUDENTS IN PULAU PINANG MALAYSIA
Authors: Jaafar R. , Akmal A. , Libasin Z. 
Published: May 2022

Ergonomics knowledge helps in its right application and contributes significantly to the general well-being and safety of students. Ergonomics in Malaysia is a relatively new concept and yet to be considered an essential component of most organizations. The purpose of this study is to examine the engineering students in Universiti Teknologi MARA Cawangan Pulau Pinang (UiTMCPP), Malaysia on their ergonomics knowledge level. Questionnaires were distributed to 246 engineering students of mechanical, civil, chemical, and electrical in UiTMCPP and the responses were analyzed using SPSS version 15. The result of their ergonomics knowledge found that the average mean score and standard deviation were 2.74 and 1.21 respectively. It shows that their ergonomics knowledge level was moderate. The average mean score of the steps taken by the university management is 4.03 which is a high level. The university management and respective school must give regular ergonomics education and training for the students as well as university staff to increase the ergonomics knowledge to a better level.
Investigation of Optimum EDM Die Sinking Parameters in Micro Dimple Machining
Authors: Jie Ying Lee , Abdul Manaf Ahmad Rosli 
Published: May 2022

Micro dimple is a standard surface structure that is commonly used in micro-moulds, micro/nano-fluid devices, micromechanical systems, and bionic functional surfaces. These microstructure surfaces can be used for optical communication systems, micro sensors, flat panel display, light emitting diode applications and others. Many researchers have performed investigations on the parameters that help in manufacturing the micro dimple. However, there is still limited research on the certain parameters that will affect the micro dimple using EDM die sinking machine. EDM die sinking process involved a tool and workpiece material which immersed in a dielectric fluid medium and use the electrical spark discharges from electric pulse generation to remove the workpiece material. The actual performance of the surface integrity and form accuracy in micro dimple by EDM die sinking machine remains unclear. Therefore, this research attempts to investigate the effect of the dimple measurement such as diameter, depth and surface roughness (Ra) to the parameters which is surface roughness (Rz), sparking gap and type of electrode. By utilising the parameters, the lab experiment was conducted, and their surface integrity, effects in micro dimple behaviour were investigated. It was discovered that the higher surface roughness (Rz), the higher current produced thus erodes the material and caused the measurement of the dimple (diameter, depth and surface roughness (Ra)) differed from the expected size. Also the findings that the surface integrity was ununiformed due to the tool worn out occurred during the electric spark process causing high material rate removal. Other than that, the material electrode which has the high thermal conductivity and melting point resulted the best form accuracy of micro dimple.
Factors influencing the duration of hospitalization of patients with schizophrenia
Authors: Velelekou Anna , Papathanasiou Ioanna V. , Alikari Victoria , Papagiannis Dimitrios , Tsaras Konstantinos , Fradelos Evangelos C 
Published: May 2022

Introduction. Severe mental disorders represent an important and large proportion of healthcare resource utilization and are associated with increased hospitalization rates and costs. Given the high percentage of total hospital days and costs associated with caring for people with psychotic disorders, a better understanding of the factors that determine the duration of hospitalization of patients with such disorders is imperative.
Purpose. The aim of the present study was to investigate socio-demographic, clinical and psychosocial characteristics of patients with schizophrenia, admitted to a Greek public psychiatric hospital, and the relationship between these characteristics and duration of hospitalization among these patients.
Methods. This is a cross-sectional study. The study sample consisted of 103 patients diagnosed with schizophrenia (F-20). The collection of data was performed by using the NEO-Five Factor Inventory, the Connor-Davidson Resilience Scale (CD-RISC25), Multidimensional Scale of Perceived Social Support, (MSPSS), Positive and Negative Syndrome Scale (PANSS), Global Assessment of Functioning scale (GAF) for assessing key personality factors, psychological resilience, social support, symptom severity and the severity of the disease, respectively. Descriptive analyses and inferential statistic methods were applied. Correlation between socio-demographic, psychosocial or clinical characteristics with the length of stay were explored. In addition, linear regression analysis was performed in order to examine predicting factors for the duration of hospitalization. All statistical analysis was performed using SPSS v.25.
Results. The mean age of the sample was 43.9 (SD = ±11.4) years, 67% of patients were men, and the mean length of hospitalization was 40.7 days. Factors found to be significantly associated with length of stay in the overall sample include the previous admissions (p=0.010), the type of admission (compulsory or voluntary) (p=0.017), bed rest (p=0.043) and duration of bed rest (p=0.002), and the existence of social support networks especially from friends (p=0.018).
Conclusions. Our findings indicate that basic psychosocial and clinical factors were associated with the duration of hospital stay. Duration of hospitalization results from a complex interface between characteristics and activities of the health system, patient, and clinician’s influence on discharge timing, which requires additional study. Our findings further warrant the need for policymakers to consider socio-demographic status, psychosocial and clinical factors when allocating resources to hospitals caring for patients with severe mental disorders.
Pharmacokinetics and bioavailability of curdione in mice by UPLC-MS/MS
Authors: Liang Qishun , Chen Tianyu , Luo Lvqi , Ma Yizhe , Wen Congcong , Huang Xueli 
Published: May 2022

AbstractA UPLC-MS/MS method was developed to determinate curdione in the mouse blood, and the pharmacokinetics of curdione in mice after intravenous (5 mg kg−1) and oral (20 mg kg−1) administration were studied. The HSS T3 column was used for separation, and column temperature was set at 40 °C. Multiple reaction monitoring (MRM) mode were used for determination of curdione. Blood samples were taken from the caudal vein of Institute of Cancer Research (ICR) mice after administration of curdione. It showed a good linear relationship in the range of 1–500 ng mL−1 (r > 0.998); the intra-day precision was <13%, the inter-day precision was <15%, and the accuracy was 90%–105%, the recovery was >77%, and the matrix effect was 97%–107%. The half-life was relatively short, and the bioavailability was 6.5%. The developed method was suitable for the pharmacokinetics of curdione in mice.
Nanoscale intergrowths in the bastnäsite–synchysite series record transition toward thermodynamic equilibrium
Authors: Ciobanu C. L. , Cook N. J. , Slattery A. D. , Ehrig K. , Liu W. Y. 
Published: May 2022

AbstractThe accelerated pace of transition to green energy technologies has led to increased demand for rare-earth elements (REEs). Fluorocarbonates from the bastnäsite [REE(CO3)F]—synchysite [CaREE(CO3)2F] group are abundant in nature and the dominant REE minerals in the >10 billion ton Olympic Dam Cu–U–Au–Ag deposit, South Australia. Intergrowths of bastnäsite and synchysite slabs at the nanometer to micron scales are the rule rather than the exception and account for structural and compositional changes between the two endmembers. This article reviews REE-fluorocarbonate mineralogy at Olympic Dam with emphasis on the significance of intergrowths among bastnäsite and synchysite for achievement of thermodynamic equilibrium. Imaging by high-angle annular dark-field scanning transmission electron microscopy (HAADF STEM) allows visualization of atomic-scale periodicity and structural modularity in REE fluorocarbonates and, in turn, determination of domain compositions, which are otherwise below the spatial resolution of microbeam methods. Despite highly irregular, such domains featuring atomic-scale intergrowths can approach the compositions of the next intermediate member phase within each host, implying formation in a system close to thermodynamic equilibrium.
Graphic Abstract
Peculiarities of electrophoretic deposition and morphology of deposited films in non-aqueous suspensions of Al2O3–Al nanopowder
Authors: Kalinina Elena G. , Rusakova Darya S. , Pikalova Elena Yu. 
Published: May 2022

The paper presents the results of a comprehensive study of the electrokinetic properties of non-aqueous suspensions of the Al2O3–Al nanopowder obtained by the method of electric explosion of wires (EEW) with 0.3 wt.% of metallic aluminum in its composition. The dependence of zeta potential on the concentration of the Al2O3–Al suspension is revealed. The nature of long-term changes in zeta potential and pH in suspensions is established. Appearance of bubbles in the deposited coatings due to the interaction of metallic aluminum particles with the liquid suspension medium during electrochemical reactions on the electrodes is defined as the main feature of the electrophoretic deposition (EPD) process in the Al2O3–Al suspensions. The influence of the suspension preparation method on the deposited coatings’ morphology is demonstrated.
Pengembangan Permainan Modifikasi Aktivitas Lari Jarak Pendek dalam Pembelajaran Atletik di SMK Negeri 1 Batu
Authors: Aslam Farid Soni , Heynoek Febrita Paulina , Fitriady Gema 
Published: May 2022

Abstract: The purpose of this development research is to develop a game modification for short distance running activities in athletic learning which is packaged in the form of a video. The method used in this research is a procedural model that refers to the research and development development model of Borg and Gall. The data analysis technique used is descriptive with a percentage. The validation results from the experts, namely: 80 percent athletic learning, 80 percent game modification, and 92.85 percent learning media can be used without improvement. From small group trials of students with a total of 85.2 percent and, as well as large group trials of students totaling 88.51 percent, it can be used without any improvement.
Abstrak: Tujuan dari penelitian pengembangan ini adalah untuk mengembangkan modifikasi permainan aktivitas lari jarak pendek dalam pembelajaran atletik yang dikemas dalam bentuk video. Metode yang digunakan dalam penelitian ini adalah model prosedural mengacu kepada model pengembangan research and development Borg and Gall. Teknik menganalisis data yang digunakan yaitu deskriptif persentase. Hasil validasi dari para ahli, yaitu: pembelajaran atletik 80 persen, modifikasi permainan 80 persen, dan media pembelajaran 92,85 persen dapat digunakan tanpa perbaikan. Dari uji coba kelompok kecil peserta didik dengan jumlah 85,2 persen dan, serta uji kelompok peserta didik (besar) berjumlah 88,51 persen dapat digunakan tanpa adanya perbaikan.