bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reveal the biomarkers and immune infiltration of dermatomyositis and explore associated diseases as well as drug molecules by bioinformatics analysis
Authors: Zhou Haonan , Peng Jie , Zong Zhen , Wu Rui , Li Hui 
Published: May 2022

Abstract
Background: Dermatomyositis (DM) is a rare autoimmune disease, which occurs with poorly understood pathogenesis. Unclear pathogenesis and similar symptoms with other inflammatory myopathies make its diagnosis hard. The study was designed to provide more details about the pathogenesis of DM, trying to identify potential biomarkers, biomarkers` associated diseases and drugs for DM`s diagnosis and treatment.Method: GSE142807, GSE1551, GSE5370, GSE39454, GSE48280 and GSE46239 datasets were selected in our study from Gene Expression Omnibus (GEO). GEO2R was used to identify differentially expressed genes (DEGs) between DM samples and controls. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene set enrichment analysis (GSEA) were conducted to identify enrichment pathways. We used Cytoscape to build up the protein-protein interaction (PPI) network. Weighted gene co-expression network analysis (WGCNA) was used to verify the preceding analysis. CIBERSORT tool was applied to analyze the immune cell infiltration patterns of DM. The receiver operating characteristic (ROC) analysis was applied to explore the crucial genes` clinical significance for DM`s diagnosis. DisGeNET database was used to identify associated diseases and Drug Signatures database (DSigDB) was used to identify associated molecule drugs.Results: DEG analysis identified 31 upregulated genes. KEGG analysis indicated that the DEGs were enriched in pathways associated close with viruses. Eight hub genes were obtained through PPI network. STAT1, IFI44L and DDX58 were identified as crucial from eight hub genes after verification of WGCNA. And we found a significantly differential infiltration of regulatory cells and macrophages in DM samples. ROC analysis demonstrated that the 3 genes had excellent clinical values and their combination model had an AUC = 1, potential to be biomarkers for DM`s diagnosis. According to the gene-disease and gene-drugs analysis, Influenza and several cancers including liver carcinoma associated close to the 3 crucial genes and several drugs like prenylamine HL60 UP and suloctidil HL60 UP were identified.Conclusions: The study identified three potential biomarkers (STAT1, IFI44L and DDX58) and revealed the immune infiltration of DM and explored out several diseases including cancer and potential drugs for DM, which might guide future diagnosis and treatment for DM.
Light interaction with natural photonic crystal: from band gap structure to Anderson localization
Authors: Patiño Claudia Patricia Barrera , Mattos Vicente Silva , D’Almeida Camila de Paula , de Oliveira Éverton Lucas , de Oliveira Lucas Orlandi , Neto Jarbas Caiado de Castro , Bagnato Vanderlei Salvador 
Published: May 2022

Abstract
In nature, we can find spectacular optical phenomena exhibited by some material when interacting with light. Complex nanostructures that have evolved to perform specific functions, such as camouflage and preservation of such materials. These structures are so sophisticated that we are, until the present time, looking for the fundamentals of those unique and interesting natural phenomena exhibited by them. Therefore, to understand all the effects related to those structures, it is necessary to combine knowledge from different scientific areas. In this report, we study the structural color observed on the wing samples of Quesada gigas, Greta oto, and Morpho cypris; three native species from South America. The optical effect observed on their wings is due to the particular photonic structure arrays present on their composition. Our interest is mainly to understand how the structural disorder influences the light interaction and consequences. For this purpose, we performed interferometric measurements, used spatiotemporal measurement that can indicate localization of light, and computational methods based on Maxwell-Bloch equations, transfer matrix and Kronig-Penney model. Here we present the results of our measurements and calculations. These results come up with promissory evidence that it is possible to measure the peculiar response of the photonic systems when the light interacts with the presence of disorder introduced in the structure on samples of natural origin. Therefore, in this work we suggest one way to detect Anderson localization in natural photonic structures. Those results allowed us to establish a correlation for similar behavior observed in electronic systems by Anderson and apply it in photonic crystals known as the light semiconductors.
Characterizing alcohol-related disordered eating behaviors in adults with binge eating
Authors: Wilkinson Megan L. , Boyajian Laura E. , Juarascio Adrienne S. 
Published: May 2022

Abstract
Purpose - Alcohol-related disordered eating behaviors (ADEBs; i.e., engagement in dietary restriction or excessive exercise before or after drinking alcohol to avoid weight gain) are associated with negative psychosocial and medical consequences. Previous research has primarily studied ADEBs among community samples. Individuals with clinically-significant binge eating may also engage in ADEBs given high rates of alcohol use and inappropriate weight-control behaviors. The current study aimed to characterize the prevalence and psychological correlates (i.e., weight and shape concerns, alcohol consumption, binge eating frequency) among individuals with clinically-significant binge eating. Methods – Participants were 166 treatment-seeking individuals who engaged in once weekly binge eating over the past three months. Participants completed a clinical interview to assess eating disorder symptoms and self-report measures of alcohol consumption patterns and ADEB engagement. Results - Over one-fourth of participants endorsed at least one ADEBs in the past three months. Participants who endorsed ADEBs reported greater alcohol consumption than participants who drank alcohol but did not endorse ADEBs, after controlling for eating disorder diagnosis. Greater frequency of ADEBs was related to higher weight and shape concerns among individuals who endorsed ADEBs in the past three months. Presence of ADEBs and ADEBs frequency were not related to binge eating frequency. Conclusion - Results suggest that clinicians treating individuals with binge eating who drink alcohol should screen for ADEBs and assess how ADEBs may contribute to an individual’s eating pathology. Further research is necessary to explain how ADEBs may maintain binge eating or affect treatment outcomes. Level of Evidence: Level V, descriptive studies
TERRA G-quadruplex stabilization as a new therapeutic strategy for multiple myeloma
Authors: Scionti Francesca , Juli Giada , Rocca Roberta , Caracciolo Daniele , Riillo Caterina , Polerà Nicoletta , Grillone Katia , Altomare Emanuela , Caparello Basilio , Arbitrio Mariamena , Alcaro Stefano , Tagliaferri Pierosandro , Tassone Pierfrancesco , Martino Maria Teresa Di 
Published: May 2022

Abstract
Background
Multiple Myeloma (MM) is a hematologic malignancy characterized by high genomic instability and telomere dysfunction is an important cause of acquired genomic alterations. Telomeric repeat-containing RNAs (TERRAs) transcripts are long non-coding RNAs (lncRNAs) involved in telomere stability through the interaction with shelterin complex. Dysregulation of TERRAs has been reported across several cancer types. We recently identified a small molecule, hit 17, which stabilizes the secondary structure of TERRA. In this study, we investigated in vitro and in vivo anti-MM activities of hit 17.
Methods
Anti-proliferative activity of hit 17 was evaluated in different MM cell lines by cell proliferation assay and apoptotic process was analyzed by flow cytometry. Gene and protein expression were detected by qRT-PCR and western blot, respectively. Microarray analysis was used to analyze transcriptome profile. The effect of hit 17 on telomeric structure was evaluated by chromatin immunoprecipitation (ChIP). Further evaluation in vivo was proceeded upon NCI-H929 and AMO-1 xenograft models.
Results
TERRA G4 stabilization induced in vitro dissociation of telomeric repeat-binding factor (TRF2) from telomeres leading to the activation of ATM-dependent DNA damage response, cell cycle arrest, proliferation block, and apoptotic death in MM cell lines. In addition, up-regulation of TERRA transcription was observed upon DNA damage and TRF2 loss. Transcriptome analysis followed by GSEA confirmed the involvement of the above-mentioned processes and other pathways such as E2F, MYC, oxidative phosphorylation, and DNA repair genes as early events following hit 17-induced TERRA stabilization. Moreover, hit 17 exerted anti-tumor activity against MM xenograft models.
Conclusion
Our findings provide evidence that targeting TERRA by hit 17 could represent a promising strategy for a novel therapeutic approach to MM.
Perceived Influence of Wearable Fitness Trackers on Eating Disorder Symptoms in a Clinical Transdiagnostic Binge Eating and Restrictive Eating Sample
Authors: Wons Olivia , Lampe Elizabeth , Patarinski Anna Gabrielle , Schaumberg Katherine , Butryn Meghan , Juarascio Adrienne S 
Published: May 2022

Abstract
Wearable fitness trackers are an increasingly popular tool for measuring physical activity (PA) due their accuracy and momentary data collection abilities. Despite the benefits of using wearable fitness trackers, there is limited research in the eating disorder (ED) field using wearable fitness trackers to measure PA in the context of EDs. Wearable fitness trackers are often underused in ED research because there is limited known about whether wearable fitness trackers negatively or positively impact PA engagement and ED symptoms in individuals with EDs. The current study aimed to assess the perceived impact wearable fitness trackers have on PA engagement and ED symptoms over a 12-week CBT treatment for 30 individuals with EDs that presented to treatment engaging or not engaging in maladaptive exercise. Participants in the maladaptive exercise group (n = 17) and non-maladaptive exercise group (n = 13) wore a fitness tracker for 12 weeks and completed questionnaires assessing participants’ perceptions of the fitness trackers’ influence on ED symptoms and PA engagement throughout treatment. Results demonstrated a small percentage of individuals perceived the fitness tracker influenced ED behaviors or PA engagement, and there were mixed results on whether participants positively or negatively perceived the fitness tracker influenced them to engage in ED behaviors or PA engagement. Although preliminary, these results demonstrate the need to continue using objective measurements of PA via wearable fitness trackers to further our understanding of the positive and negative effects of fitness trackers on clinical ED samples.
Maternal Term Pruritus and Long-Term Neuropsychiatric Morbidity in the Offspring
Authors: Levanony Omer , Sheiner Eyal , Kluwgant Dvora , Pariente Gali 
Published: May 2022

Abstract
Purpose
Pruritus during pregnancy is associated with adverse maternal, pregnancy, and neonatal outcomes. We opted to assess the association between term pruritus and long-term neuropsychiatric morbidity in the offspring.
Method
In a population-based retrospective cohort study, the incidence of long-term neuropsychiatric morbidity was compared between offspring born to women with or without pruritus at term. Neuropsychiatric morbidity was assessed up to the age of 18 years according to ICD-9 codes associated with hospitalization of the offspring. A Kaplan-Meier survival curve was used to compare cumulative neuropsychiatric morbidity incidence and Cox proportional hazards models were used to control for confounders. The study included 226,918 deliveries of which 600 (0.26%) were in women with term pruritus.
Results
Offspring born to women with pruritus exhibited a higher rate of long-term neuropsychiatric morbidity, specifically developmental and neurodegenerative disorders Kaplan-Meier survival curve demonstrated a significantly higher cumulative incidence of long-term neuropsychiatric morbidity in offspring of women with pruritus. Using several Cox proportional hazards models, being born to a woman with pruritus was independently associated with an increased risk of long-term neuropsychiatric morbidity.
Conclusion
Maternal term pruritus was found to be independently associated with long-term neuropsychiatric morbidity in the offspring.
The triglyceride glucose index: a novel and effective surrogate marker to identify alanine aminotransferase levels
Authors: Hua Yu , Yu Haiyue , Chen Shuang , Zhang Xueyao , Zhang Xingang , Sun Yingxian 
Published: May 2022

Abstract
Introducton: The triglyceride glucose index (TyG) has been proposed as a marker of insulin resistance or type 2 diabetes mellitus (T2DM). Risk of serum alanine aminotransferase (ALT) levels is increased in T2DM patients. We aimed to evaluate the association between TyG index and elevated ALT. Methods: A multicenter, cross-sectional study was conducted in China from Northeast China Rural Cardiovascular Health Study (NCRCHS), and 11,573 adults with complete data were included.Results: TyG index was positively associated with the prevalence of elevated ALT. Frequency of elevated ALT increased from the lowest to the top quartile of TyG in both sexes (p for trend <0.001). Compared with the participants in the lowest quartile of TyG, the adjusted odds ratio and 95% CIs for elevated ALT were 1.71 (1.32-2.21) and 2.46 (1.90-3.19) for those in the third and the fourth quartile of TyG (p<0.001). Compared with the first quartile of TyG, participants in the top quartile of TyG had more than 2 times risk for elevated ALT (2.38-times for men and 2.22-times for women, respectively, p<0.001). According to the ROC analysis, the optimal cut-off point of TyG for elevated ALT was 8.69 and 8.96 for men and women, respectively. Conclusions: TyG index is effective to identify individuals at risk for elevated ALT. TyG thresholds of 8.69 for men and 8.96 for women was highly sensitive for detecting elevated ALT subjects. Findings from this study underscore that TyG index may be suitable as a surrogate marker for abnormal liver enzymes in Chinese adults.
Influence of the Pilates method and anti-cellulite massage for maintaining healthy body composition parameters
Authors: DZIMBOVA TATYANA , MARINSKA RALITSA 
Published: May 2022

Abstract
The aging process leads to many changes in body composition, often without changes in body weight and body mass index (BMI). In general, with age, the percentage of body fat increases and lean body mass and bone mineral density decrease. The aim of the study was to compare basic anthropometric indicators such as weight, body fat percentage, muscle mass, skin folds and circumferences in the two groups. Two groups of subjects participated in the study. The first group of women (age 29.7 ± 6.7 years, height 165.7 ± 5.5 cm, weight 55.7 ± 2.4 kg) performed twice a week Pilates training, and the second group (age 40.3 ± 4.1 years, height 166.1 ± 4.6 cm, weight 59.1 ± 4.3 kg) are physically active women engaged in a variety of physical exercises, but conduct once a week anti-cellulite massage. Research shows that physical activity leads to benefits in terms of weight and body composition. By combining the method of body composition determination and somatotyping, the characteristic features of the body of the person could be measured and give guidelines for correction in physical activity and massage procedures. In addition, women over the age of 40 can keep their body fat percentage within normal limits by combining physical activity with anti-cellulite massage. The increase in muscle mass can continue at the age of 40 with regular exercise. Last but not least, the role of nutrition should be noted. It is extremely important for a healthy weight, but in the studied groups it is necessary to adjust the amounts of carbohydrates and proteins. Increasing the amount of carbohydrates in the form of whole grains, fruits and vegetables would contribute to increased intake of vitamins, minerals and fiber, which will have a good effect on the general condition of individuals. Reducing the amount of protein will not interfere with the normal synthesis of muscle proteins, but will support the liver, kidneys and protect the body from calcium excretion. This study provokes many more questions about the effect of both methods on weight and body composition. If it is possible to conduct planned research (cancellation of the emergency epidemic situation), it would be good to make measurements over time, which will allow to compare the effect of Pilates and anti-cellulite massage.
Differential expression of aquaporin genes and the influence of environmental hypertonicity on their expression in juveniles of air-breathing stinging catfish (Heteropneustes fossilis)
Authors: Chutia Priyambada , Saha Nirmalendu , Das Manas , Goswami Lalit Mohan 
Published: May 2022

Abstract
Aquaporins (AQPs) are a superfamily of transmembrane channel proteins that are responsible for the transport of water and some other molecules to and from the cell, mainly for osmoregulation under anisotonicity. We investigated here the expression patterns of different AQP isoforms and also during exposure to hypertonicity (300 mOsmol/L) for 48 h in juvenile stages of air-breathing stinging catfish (Heteropneustes fossilis). A total of 8 mRNA transcripts for different isoforms of AQPs and their translated proteins could be detected in the anterior and posterior regions of S1, S2, and S3 stages of juveniles of stinging catfish at variable levels. In general, more expression of mRNAs for different aqp genes was seen in the S2 and S3 juveniles than in the S1 juvenile. Most interestingly, exposure to hypertonicity of S2 juvenile for a period of 48 h led to increased expression of most of the aqp genes both at transcriptional and translational levels, except for aqp3 in the anterior and posterior regions and aqp1 in the anterior region, showing maximum expression at later stages of hypertonic exposure. Thus, it is evident that AQPs play crucial roles in maintaining the water and ionic balances under anisotonic conditions even at the early stages of development of stinging catfish as a biochemical adaptational strategy to survive, grow and reproduce in anisotonic environment.
Finite element analysis of changes in tensile strain and deformation by airbag impact in eyes of various axial lengths
Authors: Kobayashi Ayaka , Izaki Ryosuke , Fujita Hideaki , Harada Kazunori , Ozaki Hiroaki , Kadonosono Kazuaki , Uchio Eiichi 
Published: May 2022

Abstract
Purpose: Airbags have substantially reduced mortality and morbidity, while ocular injuries caused by airbags have been reported. We applied a three-dimensional finite element analysis (FEA) model we have established for evaluation of the deformation of an intact eyeball of various axial lengths induced by an airbag impact at various impact velocities.Methods: A model human eye we have created was used in simulations with an FEA program, PAM-GENERISTM (Nihon ESI, Tokyo, Japan). The airbag was set to impact eyes with various axial lengths of 21.85 mm (hyperopia), 23.85 mm (emmetropia) and 25.85 mm (myopia), at initial velocities of 30, 40, 50 and 60 m/s. Changes in the shape of the eye and the strain induced were calculated. Deformation of the eye in a cross-sectional view was displayed sequentially in slow motion.Results: We found that considerable damage, such as corneal or scleral lacerations, was observed especially at higher impact velocities, such as 50 or 60 m/s, in eyes with any axial length. Deformation was most evident in the anterior segment. The decrease rate of axial length was greatest in the hyperopic eye, followed by the myopic eye, and the emmetropic eye.Conclusions: It was shown that hyperopic eyes are most susceptible to deformation by an airbag impact in this simulation. The considerable deformation by an airbag impact on the eye during a traffic accident shown in this study might indicate the necessity of ocular protection to avoid permanent eye damage.
NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway
Authors: Dong Hui , Wan Hanxing , Gao Nannan , Lu Wei , Lu Cheng , Chen Jun , Wang Yimin 
Published: May 2022

Abstract
Plasma membrane Na+/Ca2+ exchanger 1 (NCX1) is a bidirectional ion transporter to operate in Ca2+ entry and exit modes, and TRPC1 is Ca2+-permeable membrane channels. Both play critical roles in maintaining cytosolic free Ca2+ ([Ca2+]cyt) homeostasis in mammalian cells. Although either TRPC1 channels or Ca2+ entry mode of NCX1 is implicated in several human tumorigenesis, it has not been explored for a coordination of NCX1 and TRPC1 to involve in the pathogenesis of H. pylori-associated gastric cancer (GC). The protein expression of NCX1 was significantly enhanced in human GC specimens, which correlated with tumor progression and poor survival in GC patients. TRPC1 and NCX1 proteins were parallelly enhanced，co-localized and bound in human GC cells. By a functional coupling, TRPC1 drives NCX1 to its Ca2+ entry mode to raise [Ca2+]cyt in GC cells. Moreover, CaCl2, H. pylori and their virulence factors all enhanced expressions and activities of NCX1 and TRPC1, and evoked aberrant Ca2+ entry to promote proliferation, migration, and invasion of GC cells through AKT/b-catenin pathway. Tumor growth and metastasis also depended on the enhanced expression of NCX1 in subcutaneously xenografted GC model of nude mice. Overall, our findings indicate that TRPC1/NCX1 coupling may promote H. pylori-associated GC through the Ca2+/AKT/b-catenin pathway. Since Ca2+ exit mode and Ca2+ entry mode of NCX1 play different roles under mostly physiological and pathological conditions respectively, we propose that targeting TRPC1/NCX1 coupling could be a novel strategy for selectively blocking Ca2+ entry mode to potentially treat digestive cancer.
Intracranial, intratumoral implantation of drug-releasing microdevices in patients with high grade gliomas is feasible, safe, and may predict tumor response to systemic chemotherapy
Authors: Peruzzi  , Dominas Chrstine , Fell Geoffrey , Blitz Sarah , Bernstock  , Dawood Hassan , Triggs NP Daniel , Ahn Sebastian , Bhagavatula Sharath , Tatarova Zuzana , Pannell Michael , Truman Kyla , Ball Anna , Chiocca  , Ligon Keith , Wen Patrick , Jonas Oliver 
Published: May 2022

Abstract
The lack of reliable predictive biomarkers to guide effective therapy is a major obstacle for the advancement of therapy for high grade gliomas (HGG), and particularly glioblastoma (GBM), one of the few cancers whose prognosis has not improved over the past several decades. With this pilot clinical trial we provide first in human evidence that drug-releasing intratumoral microdevices (IMD) can be safely and effectively used to obtain patient-specific, high throughput molecular and histopathological data to inform selection of drugs based on their observed antitumor effect in situ. The use of IMD is seamlessly integrated in standard surgical practice during tumor resection. None of the six enrolled patients experienced adverse events related to the IMD, and the retrieved tissue was usable for downstream analysis for 11 out of 12 retrieved specimens. Molecular analysis of the specimens provided, for the first time in humans, preliminary evidence of the robustness of the readout, with strong correlation between IMD analysis and clinic-radiological responses to temozolomide. From an investigational aspect, the amount of information obtained with IMD allows unprecedented characterization of tissue effects of any drugs of interest, within the physiological context of the intact tumor.