bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overview and clinical significance of multiple mutations in individual genes in hepatocellular carcinoma
Authors: Imamura Taisuke , Okamura Yukiyasu , Ohshima Keiichi , Uesaka Katsuhiko , Sugiura Teiichi , Ito Takaaki , Yamamoto Yusuke , Ashida Ryo , Ohgi Katsuhisa , Otsuka Shimpei , Ohnami Sumiko , Nagashima Takeshi , Hatakeyama Keiichi , Sugino Takashi , Urakami Kenichi , Akiyama Yasuto , Yamaguchi Ken 
Published: March 2022

Abstract
Multiple mutation (MM) within a single gene has recently been reported as a mechanism involved in carcinogenesis. The present study investigated the clinical significance of MMs in hepatocellular carcinoma (HCC). Two hundred twenty-three surgically resected HCCs were subjected to gene expression profiling and whole-exome sequencing. MMs in individual genes were detected in 178 samples (MM tumors: 79.8%). The remaining samples all carried a single mutation (SM tumors: 20.2%). MMs showed marked differences in their different mutational patterns, with a higher functional impact in comparison to SMs. Recurrence-free survival in the MM group was significantly worse in comparison to the SM group (P = 0.012). A Cox proportional hazard analysis revealed that MM tumor was an independent predictor for worse a prognosis (hazard ratio, 1.72; 95% confidence interval, 1.01–3.17; P = 0.045). MMs were frequently observed across in various genes, especially MUC16 (15% of samples had at least one mutation in the gene) and CTNNB1 (14%). Although the MUC16 mRNA expression of MUC16 wild-type and MUC16 SM tumors did not differ to a statistically significant extent, the expression in MUC16 MM tumors was significantly enhanced in comparison to MUC16 SM tumors (P < 0.001). In MUC16, MMs were associated with viral hepatitis, higher tumor marker levels and vascular invasion. The MUC16 MMs group showed significantly worse recurrence-free survival in comparison to the MUC16 SM group (P = 0.022), while no significant difference was observed between the MUC16 SM group and the MUC16 wild-type group (P = 0.324). In conclusion, MM was a relatively common event that may occur selectively in specific oncogenes and is involved in aggressive malignant behavior.
Sleep Duration and Risk of High Blood Pressure in Thai Adolescents: The Thai National Health Examination Survey V, 2014 (NHES-V)
Authors: Pirojsakul Kwanchai , Aekplakorn Wichai , Siwarom Sirinapa , Paksi Witchuri , Kessomboon Pattapong , Neelapaichit Nareemarn , Chariyalertsak Suwat , Assanangkornchai Savitree , Taneepanichskul Surasak 
Published: March 2022

Abstract
Background: Sleep duration has been proposed to be associated with high blood pressure. However, nationwide studies regarding the association between sleep duration and high blood pressure in adolescents are limited. This study aimed to explore the national prevalence of high blood pressure among Thai adolescents and to determine whether sleep duration is associated with high blood pressure.Methods: Data of adolescents aged 10-19 years from the Thai National Health and Examination Survey V were included. Demographic data including age, gender, height, weight, waist circumference, blood pressure, fasting blood chemistries and data on the sleep duration were collected. High blood pressure was defined based on the cut-off values from the guidelines by the American Academy of Pediatrics. Risk factors for high blood pressure were analyzed using multivariate logistic regression.Results: A total of 3539 (1803 female) adolescents were included. The prevalence of high blood pressure was 9.4% (95%CI 8.5, 10.4%). The high blood pressure group had higher BMI z-score, triglyceride, LDL-C and a lower HDL-C than those in the normotensive group. In multivariate analysis, BMI z-score was associated with high blood pressure. Interestingly, after adjusting for BMI z-score and lipid profile, an average sleep duration below 6 hours/day was associated with a 2-fold higher risk of high blood pressure compared to that of the group with an average sleep duration of more than 8 hours/day.Conclusions: High blood pressure risk was increased in participants who had an average sleep duration below 6 hours/day. This study highlights the need for promoting an adequate sleep duration in Thai adolescents to alleviate the risk of high blood pressure.
Health systems’ preparedness to provide post-abortion care: Assessment of Health Facilities in Burkina Faso, Kenya and Nigeria
Authors: Juma Kenneth , Ouedraogo Ramatou , Adjei Joshua-Amo , Sie Ali , Ouattara Mamadou , Emma-Echiegu Nkechi , Eton Joseph , Mutua Michael , Bangha Martin 
Published: March 2022

Abstract
Background: In many parts of sub-Saharan Africa (SSA), access to abortion is legally restricted, which partly contributes to high incidence of unsafe abortion. This may result in unsafe abortion-related complications that demand long hospital stays, treatment and attendance by skilled health providers. There is however, limited knowledge on the capacity of public health facilities to deliver post-abortion care (PAC), the spread of PAC services in these settings. We describe and discuss the preparedness and capacity of public health facilities to deliver complete and quality PAC services in Burkina Faso, Kenya and Nigeria. Methods: A cross-sectional survey of primary, secondary and tertiary-level public health facilities was conducted between November 2018 and February 2019 in the three countries. Data on signal functions (including information on essential equipment and supplies, staffing and training among others) for measuring the ability of health facilities to provide post-abortion services were collected and analyzed. Results: Across the three countries, fewer primary health facilities (ranging from 6.3%–12.1% in Kenya and Burkina Faso) had the capacity to deliver on all components of basic PAC services. Approximately one-third (26%–43%) of referral facilities across Burkina Faso, Kenya and Nigeria could provide comprehensive PAC services. Lack of trained staff, absence of necessary equipment and lack of PAC commodities and supplies were a main reason for inability to deliver specific PAC services (such as surgical procedures for abortion complications, blood transfusion and post-PAC contraceptive counselling). Further, the lack of capacity to refer acute PAC cases to higher-level facilities was identified as a key weakness in provision of post-abortion care services. Conclusions: Our findings reveal considerable gaps and weaknesses in the delivery of basic and comprehensive PAC within the three countries, linked to both the legal and policy contexts for abortion as well as broad health system challenges in the countries. There is a need for increased investments by governments to strengthen the capacity of primary, secondary and tertiary public health facilities to deliver quality PAC services, in order to increase access to PAC and avert preventable maternal mortalities.
Effect of standardized education and supervision on pregnancy outcomes of women with gestational diabetes mellitus
Authors: zhou yanna , Gan guiping 
Published: March 2022

Abstract
Background: To evaluate the effect of knowledge and practice education on reducing adverse pregnancy outcomes in women with gestational diabetes mellitus (GDM). Methods: Prospective case-control studies were conducted, women with GDM who had randomly assigned into control group (168 cases) and intervention group (182 cases). In the control group, routine prenatal examination education was conducted, while in the intervention group, in addition to routine prenatal education, professional used video, WeChat public platform to regularly push knowledge and behavior education to women with GDM and supervise the implementation of actions. The pregnancy outcomes of the two groups were compared.Results: ①fasting blood glucose ,postprandial blood glucose in two hours, glycosylated hemoglobin of the intervention group was significantly lower than that of the control group (P < 0.05); ② in the intervention group ,the TPOAb positive rate was lower and FT4 was higher than that in the control group (P > 0.05); the vaginal pH value was significantly higher than that of the control group, TSH, the weight gain during pregnancy ,the incidences of preeclampsia, polyhydramnios, preterm premature rupture of membranes, premature rupture of membranes, cesarean section, puerperal infection, amniotic fluid pollution, chorioamnionitis, macrosomia, large for gestational age infants, neonatal care, neonatal hypoglycemia, neonatal pneumonia, the detection rates of BV, Candida, mycoplasma, Streptococcus agalactiae, cleanliness III / IV, and the positive rates of hydrogen peroxide, sialidosidase, and leukocyte esterase were significantly lower than those in the control group (P < 0.05)；The rates of Trichomonas, Neisseria gonorrhoeae, Chlamydia, the incidences of oligohydramnios, FGR, placenta previa, placental abruption, fetal distress in utero, postpartum hemorrhage, preterm labor, neonatal Apgar score less than 7 in one minute, neonatal defect, neonatal death, small for gestational age, hyperbilirubinemia were lower than those in the control group (P > 0.05). Conclusion: women with GDM supervise their self-management during pregnancy, which can effectively control the blood glucose level, maintain the vaginal microecological balance, and reduce the occurrence of adverse pregnancy outcomes.
Evaluation of dissolved oxygen sinks in water-stirring setups used in reaeration experiments
Authors: Ferreira M. de S. , da Luz M. S. , Silveira A. , Pinheiro H. D. , Gonçalves J. C. de S. I. 
Published: March 2022

AbstractDissolved oxygen (DO) is an essential parameter of water quality, and the surface reaeration process (K2) is the main source of DO in aquatic ecosystems. On a laboratory scale, reaeration measurements are performed using water-stirring setups (WSSs), but possible physical DO sinks are not considered in the WSSs. This paper aimed to assess the presence of physical DO sinks in WSSs. The experiments were carried out in four WSSs: circular hydraulic channel (CHC); straight hydraulic channel (SHC); turbine-stirred tank (TST); and oscillating grid tank (OGT). The physical DO sink was estimated by inserting a first-order term (K3, day−1) in the classic equation of DO balance. All WSSs presented physical DO sinks during the reaeration experiments: SHC (mean K3 = 4.58 day−1) &gt; TST (mean K3 = 1.30 day−1) &gt; CHC (mean K3 = 1.12 day−1) &gt; OGT (mean K3 = 0.13 day−1). Physical DO sinks were originated from low-pressure zones located in WSSs, which was confirmed by the negative correlation (r = −0.78) between K3 and the static pressure in the suction pipeline of the SHC. This study presented additional information about identifying, and quantifying, physical DO sinks in WSSs, which are essential to produce a correct estimation of the K2.
Antidiabetic and Renoprotective Effects of Ethyl Acetate Fraction of Mimosa
pudica Linn. Leaves Extract in Streptozotocin-Induced Diabetic Mice
Authors: Lan Pham Thi , Trang Tran Thi Huyen , Thom Vu Thi , Minh Phan Hong , Vui Dao Thi , Hang Pham Thi Nguyet , Tung Bui Thanh 
Published: March 2022

Background:
Traditional medicine has used Mimosa pudica Linn. for diabetes treatment.
This study evaluates the antidiabetic and renoprotective effects of the ethyl acetate fraction of M.
pudica leaves on streptozotocin-induced diabetes in mice.

Methods:
The cold maceration method was used to extract M. pudica leaves with 80% ethanol at
room temperature. Ethyl acetate (EtOAc) fraction was obtained from the M. pudica leaves extract
by successively partitioning with different solvents. Mice were induced diabetes type 2 by streptozotocin
(STZ) at a low dose and treated with EtOAc fraction of M. pudica leaves at 50 mg/kg and
100 mg/kg b.w for 60 days. After 24 hours of the final dose of therapy, the mice were sacrificed to
extract blood and kidney tissues for biochemical and histopathological analysis.

Results:
The EtOAc fraction of M. pudica leaves showed strong activity in improving glucose
concentration in the oral glucose tolerance test. Our results showed that EtOAc fraction significantly
decreased levels of glucose, total cholesterol (TC), low-density lipoprotein (LDL), and triglyceride
(TG) and increased the level of high-density lipoprotein (HDL), and protected kidneys against
damage in mice. EtOAc fraction also increased the levels of antioxidant enzymes, including catalase
(CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), and decreased malondialdehyde
(MDA) formation and pro-inflammatory cytokines (IL-1β and TNF-α) in kidney tissues.
Moreover, the renoprotective effect was also observed in the histopathological analysis.

Conclusion:
Our findings support the fact that the EtOAc fraction of M. pudica leaves has potent
anti-diabetic nephropathy activity by decreasing pro-inflammatory cytokines and improving antioxidant
levels.
Developing National Identity Scale: As Indonesian Case
Authors: Baydhowi . , Purwono Urip , Siswadi Ahmad Gimmy Prathama , Ali Moondore Madalina 
Published: March 2022

Background:
National identity is an important concept to be developed, so it is necessary to have a measurement instrument that have evidence of validity and reliability. Thus, the accuracy of the information obtained through measurement of this instrument will be guaranteed.

Objective:
This article aims to examine the psychometric property of the national identity scale to fulfill the standardization of accurate measurement.

Methods:
In this study, three analytical approaches were used, those are content validity involving eight subject matter experts (SME), confirmatory factor analysis (CFA) and reliability analysis. For the reliability and CFA approach, 300 students were used as samples who were taken through convenience sampling technique.

Results:
The results of the analysis show that out of the 20 items compiled in the content validity analysis, one item is inadequate because it has a below standard score on Aiken's coefficient of validity. This one item then revised again to be included in the tryout and data collection for the next stage of analysis. The results of the CFA analysis show that the model and data are fit and there are two inadequate items, while the contribution of the three factors on the national identity scale is significant. In the third analysis, it was found that an adequate coefficient reliability had an effect on the low Standard Error of Measurement (SEM).

Conclusion:
Based on the results of the analysis, it can be concluded that the Indonesian national identity scale has fulfilled good psychometric properties. It can be proven from evidence validity base on content, confirmatory factor analysis (CFA) and reliability analysis.
APPLICATION OF THE PEER TEACHING METHOD TO INCREASE LEARNING OUTCOMES OF BASIC ELECTRICITY AND ELECTRONICS
Authors: Astuti Widi 
Published: March 2022

This research is aimed to describe the application of the Peer Teaching Method to improve the learning outcomes of Basic Electrical and Electronics in class X AV-B SMKN 1 Saptosari, Gunungkidul. This research is a classroom action research that begins with a pretest to determine the basic score after learning is carried out before using the Peer Teaching method. This research consists of two cycles. The research was conducted at SMKN 1 Saptosari, the research subjects were 34 students of class X AV-B Audio Video. Methods of data collection using observation sheets, performance sheets / short description questions, and student response questionnaire sheets off the Peer Teaching method. Data analysis used descriptive qualitative analysis technique. The results showed that the Peer Teaching method could improve student learning outcomes. Improved results can be seen from the increase in the achievement of student learning completeness in each cycle. The achievement of student learning completeness in the pre-cycle is 41.17% (15 students complete). After being subjected to action in the first cycle with the peer tutor method, the student's mastery achievement increased to 85.29% or 29 students had met the KKM. Student learning activities also increase, because active students can easily ask questions in detail with their tutor friends. Especially when the teacher gives questions to work on, students scramble to be able to do it in front of the class. Furthermore, in the second cycle, the achievement of student learning completeness reached 91.18% or increased to 31 students who had reached the KKM. Three students who have not KKM are almost close to the value of 7.5 (KKM) with the lowest score of 7.00. Keywords: peer teaching method, learning outcomes, basic electricity and electronics
PENERAPAN COOPERATIVE LEARNING DALAM PENGAJARAN SPEAKING PADA MATERI ASKING FOR AND GIVING OPINION
Authors: Sulistyorini Nur Endah 
Published: March 2022

AbstrakBelajar bahasa Inggris sebagai bahasa asing merupakan proses pembelajaran jangka panjang. Proses tersebut meliputi menginternalisasikan budaya pada proses pengajaran. Dengan kata lain, belajar bahasa membutuhkan waktu dan pembiasaan yang berkesinanbungan. Dalam proses tersebut motivasi belajar siswa dalam pembelajaran bahasa memiliki hubungan yang erat dengan kemampuan mereka tentang bahasa target. Mengajarkan bahasa yaitu menciptakan suasana berkomunikasi yang senantiasa merangsang keingintahuan mereka tentang bahasa target.Dalam proses belajar mengajar bahasa Inggris, guru menghadapi beberapa kondisi dimana peserta didik memiliki karakteristik dan minat masing-masing. Mereka cenderung memiliki tingkat motivasi belajar yang berbeda dalam proses pembelajaran bahasa Inggris. Kondisi ini terkait dengan latar belakang mereka dimana hal tersebut mempengaruhi motivasi belajar bahasa Inggris. Dengan kondisi tersebut, perlu adanya kerja sama antar siswa untuk saling menguatkan dan memotivasi dalam proses pembelajaran bahasa. Guru harus mencoba berbagai model pembelajaran yang paling cocok untuk memecahkan masalah di kelasnya sehingga proses belajar mengajar berjalan sesuai harapan.Tulisan ini membahas tentang penerapan model pembelajaran Cooperative Learning, bagaimana langkah-langkah pembelajarannya dan apakah model pembelajaran tersebut dapat menciptakan lingkungan belajar yang efektif, aktif dan menyenangkan.
Suitability of the Unified Theory of Acceptance and Use of Technology 2 Model for Predicting mHealth Acceptance Using Diabetes as an Example: Qualitative Methods Triangulation Study
Authors: Schretzlmaier Patrik , Hecker Achim , Ammenwerth Elske 
Published: March 2022

BackgroundIn recent years, the use of mobile health (mHealth) apps to manage chronic diseases has increased significantly. Although mHealth apps have many benefits, their acceptance is still low in certain areas and groups. Most mHealth acceptance studies are based on technology acceptance models. In particular, the Unified Theory of Acceptance and Use of Technology 2 (UTAUT2) model was developed to predict technology acceptance in a consumer context. However, to date, only a few studies have used the UTAUT2 model to predict mHealth acceptance and confirm its suitability for the health sector. Thus, it is unclear whether the UTAUT2 model is suitable for predicting mHealth acceptance and whether essential variables for a health-related context are missing.ObjectiveThis study aims to validate the suitability of UTAUT2 for predicting mHealth acceptance.MethodsIn this study, diabetes was used as an example as mHealth apps are a significant element of diabetes self-management. In addition, diabetes is one of the most common chronic diseases affecting young and older people worldwide. An explorative literature review and guided interviews with 11 mHealth or technology acceptance experts and 8 mHealth users in Austria and Germany were triangulated to identify all relevant constructs for predicting mHealth acceptance. The interview participants were recruited by purposive sampling until theoretical saturation was reached. Data were analyzed using structured content analysis based on inductive and deductive approaches.ResultsThis study was able to confirm the relevance of all exogenous UTAUT2 constructs. However, it revealed two additional constructs that may also need to be considered to better predict mHealth acceptance: trust and perceived disease threat.ConclusionsThis study showed that the UTAUT2 model is suitable for predicting mHealth acceptance. However, the model should be extended to include 2 additional constructs for use in the mHealth context.
Effect of Temperature and Drying Time on Angiostensin Converting Enzyme (ACE) Inhibitor and Antioxidant Activity of Soybean (Glycine max L.)- Jackbean (Canavalia ensiformis L.) Mix Grains Tempeh Flour
Authors: Puspa Diah Ayu Puspa , Ningrum Andriati , Anggrahini Sri 
Published: March 2022

The increase of hypertension sufferers encourage the development of functional foods. The presence of tempeh mixed grains of soybean and jackbean contains bioactive peptides as antihypertensive and antioxidant agents encourage the development of functional food such as tempeh flour of mixed grains. The research was aimed to investigate the effect of temperature and drying time on functional properties, asam amino acid profile, and isoflavone compounds. The different drying temperatures (50oC and 70oC) and drying time (6; 8; and 10 h) were applied on soybean and jackbean tempeh (1:1) w/w) for making the mixed grains tempeh flour. The Angiotensin Converting Enzyme (ACE) inhibitor activity, antioxidant activity, and total phenolic, amino acids profile, and isoflavone content of the flour were analyzed. The result showed that the best tempeh flour of mixed grains was obtained by drying at 70°C for 8 h with ACE inhibitor activity of 86.04%, antioxidant activity of 55.34% and total phenolic of 3.98 mg/g DB. This flour contained amino acids related to ACE-Inhibitor peptide precursors (glutamic acid and leucine), antioxidant peptide precursors (phenylalanine and histidine) as well as isoflavone compounds (daidzein and genistein). This finding reveals that drying temperature and time affected the functional properties, amino acids profile and isoflavone compounds.
Changes of Physicochemical and Microbiological Properties of Blended Sweet and Sour Pomegranate Juices During Refrigerated Storage
Authors: Boussaa Faten , Zaouay Faten , Mars Messaoud 
Published: March 2022

Physicochemical and microbiological properties changes of new beverages combining sour (‘Garoussi 2’, ‘Mezzi 1’, ‘Mezzi 2’) and sweet (‘Gabsi’, ‘Tounsi’) pomegranate juices with different proportions (10% and 20% of sour juices) were studied over a 14 days refrigerated storage. The sour juices have proved to be very rich in total soluble solids, total anthocyanin and total phenolic contents. However, these juices are not appreciated by panellists because of high acidity content. The juice blends were characterized by an attractive red colour, acceptable acidity, higher total soluble solids content, higher total phenol and anthocyanin contents compared to sweet juices. The juice blending was found to be very effective in reducing the browning and improving the appearance of beverages during storage. High microbial loads of juice samples stored up to 14 days indicate their poor microbiological quality. The beverage based on Gabsi and Garoussi 2 (10 % and 20 % proportions) presented better nutritional and microbiological stability during storage leading to their high consumer acceptance. It was concluded that conservation of juice samples should not exceed 7 days in order to have healthy value-added beverages.