bluuebunny/crossref_metadata_2025_split huggingface dataset:
Удосконалення організаційно-управлінського забезпечення оздоровчо-рекреаційної рухової активності осіб зрілого віку
Authors: Акімова Марія , Андрєєва Олена , Мічуда Юрій 
Published: July 2022

Анотація. Протягом останніх років авторитетні міжнародні організації звертають увагу урядів держав на необхідність створення сприятливих умов для заохочення громадян до регулярної рухової активності. Мета. Обґрунтувати та розробити модель удосконалення організаційно-управлінських умов розвитку оздоровчо-рекреаційної рухової активності на основі використання засобів оздоровчих видів гімнастики для підвищення рівня залученості осіб зрілого віку до рухової активності, покращення їхнього фізичного стану. Методи. Аналіз фахової науково-методичної літератури, документальних матеріалів, метод порівняння та зіставлення, логіко-теоретичний, структурно-функціональний, системний аналіз, соціологічні методи дослідження, педагогічне спостереження, моделювання, методи математичної статистики. Результати. Вивчаючи чинники, що лімітують ефективність організаційно-управлінського забезпечення розвитку оздоровчо-рекреаційної рухової активності з використанням засобів оздоровчих видів гімнастики в Україні, встановлено, що найбільше обмежує розвиток низький рівень взаємодії організацій, що надають послуги з оздоровчих видів гімнастики, неналежний рівень впровадження наукових досягнень, технологій у практику оздоровчої діяльності та недостатня матеріальна база. Результатом досліджень стала розроблена модель удосконалення організаційно-управлінських умов розвитку оздоровчо-рекреаційної рухової активності з використанням засобів оздоровчих видів гімнастики в Україні, для чого з’ясовано необхідність удосконалення організаційно-управлінських умов розвитку; сформульовано мету та головні завдання моделі; прогнозовано очікувані результати її реалізації. Модель включає три етапи: обґрунтування необхідності удосконалення організаційно-управлінських умов, діагностика організаційно-управлінського забезпечення, прийняття управлінських рішень. Реалізація моделі передбачає розробку програми, яка включає певні цілі та завдання. Спроектованими цілями були: «Розробка довгострокової програми зміцнення здоров’я населення»; «Завоювання ринку»; «Забезпечення організаційно-управлінських умов розвитку оздоровчо-рекреаційної рухової активності з використанням оздоровчих видів гімнастики». Для реалізації окреслених цілей та завдань сформовано ієрархічну структуру основних робіт програми: «Організація програми»; «Впровадження програми»; «Контроль виконання програми».
Ключові слова: оздоровчо-рекреаційна рухова активність, модель, особи зрілого віку, оздоровчі види гімнастики.
Формування мотивації до занять з фізичного виховання студентів закладів вищої освіти засобами гімнастики
Authors: Колеснік Ірина , Терещенко Іван , Левчук Тамара 
Published: July 2022

Анотація. Як зазначають науковці, фізичне виховання, будучи складовою частиною системи виховання майбутніх фахівців, відіграє важливу роль у збереженні та зміцненні здоров’я студентів, підвищенні фізичної працездатності, збільшенні тривалості життя, формуванні професійно важливих якостей особистості. У статті розкрито проблему сучасної системи фізичного виховання студентів закладів вищої освіти. Недостатня дослідженність особливостей формування мотивації у юнаків і дівчат, що навчаються у навчальному закладі, і значущість цього питання для ефективної диференційованої організації фізичного виховання студентів доводить актуальність цього питання. Мета. Вивчення мотивів та інтересів студентів до занять з фізичного виховання, зокрема гімнастикою, під час навчання у закладі вищої освіти. Методи. Теоретичний аналіз літературних джерел, анкетування, методи математичної статистики. Результати. Серед молоді недостатньо сформована мотивація до занять фізичними вправами, тому постійно ведеться активний пошук нових форм і методів підвищення якості фізичного виховання майбутніх студентів закладів вищої освіти. На сьогодні проблема мотивації досить актуальна і висвітлена у цілому ряді робіт. У системі фізичного виховання гімнастика займає особливе місце, адже вона є одним із основних засобів для зміцнення здоров’я, фізичного розвитку, загартування організму, формування життєво необхідних рухових умінь та навичок, одним з універсальних засобів фізичного виховання. Це система специфічних вправ і методичних прийомів, які використовують з метою фізичного виховання, освіти, спортивної підготовки, оздоровлення, відновлення, лікування тощо. Вона сприяє розвитку та удосконаленню таких фізичних якостей, як швидкість, витривалість, гнучкість, спритність, сила, координаційні здібності.
Ключові слова: інтерес, пріоритети, гімнастика, засоби, мотиви.
A Turn-On Fluorescent Chemosensor for Cyanide Ion Detection in Real Water Samples
Authors: Shi Qing , Wu Shou-Ting , Shen Lingyi , Zhou Tao , Xu Hong , Wang Zhi-Yong , Yang Xian-Jiong , Huang Ya-Li , Zhang Qi-Long 
Published: July 2022

We have designed and synthesized a novel simple colorimetric fluorescent probe with aggregation-induced emission (AIE) properties. Probe 5-(4-(diphenylamine)phenyl) thiophen-2-formaldehyde W exhibited a turn-on fluorescent response to cyanide ion (CN−), which induces distinct visual color changes. Probe W exhibited a highly selective and sensitive ratiometric fluorescence response for the detection of CN− over a wide pH range (4–11) and in the presence of common interferents. The linear detection of CN− over the concentration range of 4.00–38.00 µM (R2 = 0.9916, RSD = 0.02) was monitored by UV-Vis absorption spectrometry (UV-Vis) with the limit of detection determined to be 0.48 µM. The linear detection of CN− over the concentration range of 8.00–38.00 µM was examined by fluorescence spectrophotometry (R2 = 0.99086, RSD = 0.031), and the detection limit was found to be 68.00 nM. The sensing mechanisms were confirmed by 1H NMR spectroscopic titrations, X-ray crystallographic analysis, and HRMS. Importantly, probe W was found to show rapid response, high selectivity, and sensitivity for cyanide anions in real water samples, over the range of 100.17∼100.86% in artificial lake water and 100.54∼101.64% in running water by UV-Vis absorption spectrometry, and over the range of 99.42∼100.71% in artificial lake water and 100.59∼101.17% in running water by fluorescence spectrophotometry. Importantly, this work provides a simple and effective approach which uses an economically cheap and uncomplicated synthetic route for the selective, sensitive, and quantitative detection of CN− ions in systems relevant to the environment and health.
Screening of the Key Genes and Signalling Pathways for Diabetic Nephropathy Using Bioinformatics Analysis
Authors: Li Zukai , Feng Junxia , Zhong Jinting , Lu Meizhi , Gao Xuejuan , Zhang Yunfang 
Published: July 2022

BackgroundThis study aimed to identify biological markers for diabetic nephropathy (DN) and explore their underlying mechanisms.MethodsFour datasets, GSE30528, GSE47183, GSE104948, and GSE96804, were downloaded from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified using the “limma” package, and the “RobustRankAggreg” package was used to screen the overlapping DEGs. The hub genes were identified using cytoHubba of Cytoscape. Logistic regression analysis was used to further analyse the hub genes, followed by receiver operating characteristic (ROC) curve analysis to predict the diagnostic effectiveness of the hub genes. Correlation analysis and enrichment analysis of the hub genes were performed to identify the potential functions of the hub genes involved in DN.ResultsIn total, 55 DEGs, including 38 upregulated and 17 downregulated genes, were identified from the three datasets. Four hub genes (FN1, CD44, C1QB, and C1QA) were screened out by the “UpSetR” package, and FN1 was identified as a key gene for DN by logistic regression analysis. Correlation analysis and enrichment analysis showed that FN1 was positively correlated with four genes (COL6A3, COL1A2, THBS2, and CD44) and with the development of DN through the extracellular matrix (ECM)–receptor interaction pathway.ConclusionsWe identified four candidate genes: FN1, C1QA, C1QB, and CD44. On further investigating the biological functions of FN1, we showed that FN1 was positively correlated with THBS2, COL1A2, COL6A3, and CD44 and involved in the development of DN through the ECM–receptor interaction pathway. THBS2, COL1A2, COL6A3, and CD44 may be novel biomarkers and target therapeutic candidates for DN.
Subclinical Thyrotoxicosis and Cardiovascular Risk: Assessment of Circulating Endothelial Progenitor Cells, Proangiogenic Cells, and Endothelial Function
Authors: Phowira Jason , Bakhashab Sherin , Doddaballapur Anuradha , Weaver Jolanta U. 
Published: July 2022

BackgroundSubclinical thyrotoxicosis (SCT) is defined by low or undetectable thyroid-stimulating hormones and normal thyroid hormones. The treatment of SCT is uncertain despite being associated with increased cardiovascular risk (CVR) and mortality. Circulating endothelial progenitor cells (cEPCs) and circulating angiogenic cells (CACs) have been found to be reduced in conditions with CVR. We aimed to evaluate whether endothelial function and cEPC and CAC counts were reduced in SCT and to study the in vitro effect of triiodothyronine (T3) on proangiogenic cell (PAC) function from young healthy controls.MethodscEPCs (quantified by flow cytometry, 20 SCT/20 controls), CACs following in vitro cultures (15 SCT/14 controls), paracrine function of CACs, endothelial function by flow-mediated dilation (FMD, 9 SCT/9 controls), and the effect of T3 on apoptosis and endothelial nitric oxide synthase (eNOS) expression in PACs were studied.Resultsp &lt; 0.001, CD133+/VEGFR-2+ 0.4 (0.0–0.7) vs. 0.6 (0.0–4.6), p = 0.009, CD34+/VEGFR-2+ 0.3 (0.0–1.0) vs. 0.7 (0.1–4.9), p = 0.002; while CAC count was similar. SCT predicted a lower cEPC count after adjustment for conventional CVR factors. FMD was lower in SCT subjects versus controls (% mean ± SD, 2.7 ± 2.3 vs. 6.1 ± 2.3, p = 0.005). In vitro studies showed T3 increased early apoptosis and reduced eNOS expression in PACs.ConclusionsIn conclusion, SCT is associated with reduced cEPC count and FMD, confirming increased CVR in SCT. Future outcome trials are required to examine if treatment of this subclinical hyperactive state improves cardiovascular outcome.Clinical Trial Registrationhttp://www.controlled-trials.com/isrctn/, identifier ISRCTN70334066.
Impact of a water-sediment regulation scheme on nutrient variations at the Lijin station of the Yellow River
Authors: Li Chang , Wang Zhili , Liu Huaixiang , Zuo Liqin , Lu Yan , Wu Pan , Lu Yongjun 
Published: July 2022

The water-sediment regulation scheme (WSRS) imposed on dams throughout the Yellow River not only alleviates siltation in the downstream section but also alters the nutrient characteristics, which indirectly affects the enrichment of nutrients in the estuary. Nevertheless, the long-term changes in the nutrient contents and their causes in the lower Yellow River (LYR) remain unclear, and the nutrients characteristics during the years with and without WSRS have yet to be compared. Therefore, the purpose of this study was to explore the variations in the nutrient contents and limitations at the Lijin station on the LYR over the past decade, especially during the annual WSRS period, and to compare the water quality characteristics at Lijin between the years with and without WSRS. The results reveal that WSRS significantly changed the seasonal nutrient concentrations (nitrogen, phosphorus and silicon) at the Lijin station. The fluxes of these nutrients during WSRS (excluding 2016 and 2017) accounted for 11.64–40.63% of the total annual fluxes. The N concentration in the LYR was higher than that in some global rivers, while the concentrations of dissolved inorganic phosphorus (DIP) and dissolved silica (DSi) were lower than the average levels in other rivers. In addition, higher values of dissolved inorganic nitrogen (DIN), DSi and the Redfield ratio indicated that the growth of phytoplankton at the Lijin station was strongly restricted by P. However, during the 2 years without WSRS (2016 and 2017), the proportions of the nutrient fluxes in June were less than 66% of those in the WSRS period in other years. Additionally, there was a potential Si limitation in June in these 2 years. Furthermore, due to the occurrence of floods upstream of the Yellow River and the low-level operation of the Xiaolangdi Reservoir, the fluxes of nutrients during WSRS in 2018 were approximately 0.90–4.20 times those during the same period in 2009–2015 and 6.30–35.76 times those in June 2016 and June 2017. This study shows that WSRS effectively changes the nutrient balance in the LYR and provides a reference for the multi-objective collaborative optimization of WSRS to improve siltation and control flood in the LYR.
Comprehensive Analysis Identifies and Validates the Tumor Microenvironment Subtypes to Predict Anti-Tumor Therapy Efficacy in Hepatocellular Carcinoma
Authors: Zhang Haohan , Yao Yi , Wu Jie , Zhou Jin , Zhao Chen , He Junju , Xu Bin 
Published: July 2022

ObjectiveThe objective of this study was to explore and verify the subtypes in hepatocellular carcinoma based on the immune (lymphocyte and myeloid cells), stem, and stromal cells in the tumor microenvironment and analyze the biological characteristics and potential relevance of each cluster.MethodsWe used the xCell algorithm to calculate cell scores and got subtypes by k-means clustering. In the external validation sets, we verified the conclusion stability by a neural network model. Simultaneously, we speculated the inner connection between clusters by pseudotime trajectory analysis and confirmed it by pathway enrichment, TMB, CNV, etc., analysis.ResultAccording to the results of the consensus cluster, we chose k = 4 as the optimal value and got four different subtypes (C1, C2, C3, and C4) with different biological characteristics based on infiltrating levels of 48 cells in TME. In univariable Cox regression, the hazard ratio (HR) value of C3 versus C1 was 2.881 (95% CI: 1.572–5.279); in multivariable Cox regression, we corrected the age and TNM stage, and the HR value of C3 versus C1 was 2.510 (95% CI: 1.339–4.706). C1 and C2 belonged to the immune-active type, C3 and C4 related to the immune-insensitive type and the potential conversion relationships between clusters. We established a neural network model, and the area under the curves of the neural network model was 0.949 in the testing cohort; the same survival results were also observed in the external validation set. We compared the differences in cell infiltration, immune function, pathway enrichment, TMB, and CNV of four clusters and speculated that C1 and C2 were more likely to benefit from immunotherapy and C3 may benefit from FGF inhibitors.DiscussionOur analysis provides a new approach for the identification of four tumor microenvironment clusters in patients with liver cancer and identifies the biological differences and predicts the immunotherapy efficacy between the four subtypes.
Characterization of novel small non-coding RNAs and their modifications in bladder cancer using an updated small RNA-seq workflow
Authors: Su Zhangli , Monshaugen Ida , Klungland Arne , Ougland Rune , Dutta Anindya 
Published: July 2022

Background: Bladder cancer (BLCA) is one of the most common cancer types worldwide. The disease is responsible for about 200,000 deaths annually, thus improved diagnostics and therapy is needed. A large body of evidence reveal that small RNAs of less than 40 nucleotides may act as tumor suppressors, oncogenes, and disease biomarkers, with a major focus on microRNAs. However, the role of other families of small RNAs is not yet deciphered. Recent results suggest that small RNAs and their modification status, play a role in BLCA development and are promising biomarkers due to their high abundance in the exomes and body fluids (including urine). Moreover, free modified nucleosides have been detected at elevated levels from the urine of BLCA patients. A genome-wide view of small RNAs, and their modifications, will help pinpoint the molecules that could be used as biomarker or has important biology in BLCA development.Methods: BLCA tumor tissue specimens were obtained from 12 patients undergoing transurethral resection of non-muscle invasive papillary urothelial carcinomas. Genome-wide profiling of small RNAs less than 40 bases long was performed by a modified protocol with TGIRT (thermostable group II reverse transcriptase) to identify novel small RNAs and their modification status.Results: Comprehensive analysis identified not only microRNAs. Intriguingly, 57 ± 15% (mean ± S.D.) of sequencing reads mapped to non-microRNA-small RNAs including tRNA-derived fragments (tRFs), ribosomal RNA-derived fragments (rRFs) and YRNA-derived fragments (YRFs). Misincorporation (mismatch) sites identified potential base modification positions on the small RNAs, especially on tRFs, corresponding to m1A (N1-methyladenosine), m1G (N1-methylguanosine) and m22G (N2, N2-dimethylguanosine). We also detected mismatch sites on rRFs corresponding to known modifications on 28 and 18S rRNA.Conclusion: We found abundant non-microRNA-small RNAs in BLCA tumor samples. Small RNAs, especially tRFs and rRFs, contain modifications that can be captured as mismatch by TGIRT sequencing. Both the modifications and the non-microRNA-small RNAs should be explored as a biomarker for BLCA detection or follow-up.
An innovative therapeutic educational program to support older drivers with cognitive disorders: Description of a randomized controlled trial study protocol
Authors: Delphin-Combe Floriane , Coste Marie-Hélène , Bachelet Romain , Llorens Mélissa , Gentil Claire , Giroux Marion , Paire-Ficout Laurence , Ranchet Maud , Krolak-Salmon Pierre 
Published: July 2022

Older drivers face the prospect of having to adjust their driving habits because of health problems, which can include neurocognitive disorders. Self-awareness of driving difficulties and the interaction between individual with neurocognitive disorders and natural caregiver seem to be important levers for the implementation of adaptation strategies and for the subsequent voluntary cessation of driving when the cognitive disorders become too severe. This study aims to evaluate an educational program for patient/natural caregiver dyads who wish to implement self-regulation strategies in driving activity, and to improve self-awareness of driving ability. The ACCOMPAGNE program is based on seven group workshops, which target the dyad. The workshops deal with the impact of cognitive, sensory and iatrogenic disorders on driving. They tackle questions about responsibility, and about autonomy and social life. They also provide alternative solutions aimed at maintaining outward-looking activities even if driving is reduced or stopped. A randomized controlled trial is planned to evaluate the effectiveness of the program 2 months and 6 months after inclusion, and to compare this to the effectiveness of conventional approaches. The main outcome of this trial (i.e., the implementation of self-regulated driving strategies), will be measured based on scores on the “Current Self-Regulatory Practices” subscale of the Driver Perception and Practices Questionnaire. The Driving Habits Questionnaire will be used to measure secondary outcomes (indicators of driving changes; indicators of changes in mood, quality of life and caregiver burden; and self-awareness of driving abilities). Indicators will be collected for both patients and natural caregivers. This cognitive, social and psychological program should allow older individuals with cognitive disorders to drive more safely, and help to maintain the quality of life and mood of both patient and natural caregiver despite driving limitations. The patient's care path would be optimized, as he/she would become an actor in the process of giving up driving, which will, most certainly, be needed at some point in the progress of neurocognitive disorders. This process ranges from becoming aware of driving difficulties, to implementing self-regulation strategies, through to complete cessation of driving when necessary.Clinical trial registration numberNCT04493957.
Citicoline Treatment in Acute Ischemic Stroke: A Randomized, Single-Blind TMS Study
Authors: Premi Enrico , Cantoni Valentina , Benussi Alberto , Gilberti Nicola , Vergani Veronica , Delrio Ilenia , Gamba Massimo , Spezi Raffaella , Costa Angelo , Padovani Alessandro , Borroni Barbara , Magoni Mauro 
Published: July 2022

BackgroundRecent research on animal models of ischemic stroke supports the idea that pharmacological treatment potentially enhancing intrinsic brain plasticity could modulate acute brain damage, with improved functional recovery. One of these new drugs is citicoline, which could provide neurovascular protection and repair effects.ObjectivesThe objective of this randomized, single-blind experimental study was to evaluate whether the treatment with Rischiaril® Forte was able to restore intracortical excitability measures, evaluated through transcranial magnetic stimulation (TMS) protocols, in patients with acute ischemic stroke.MethodsPatients with acute ischemic stroke were recruited and assigned to an eight-week therapy of standard treatment (control group - CG) or CDP-choline (Rischiaril® Forte, containing 1,000 mg of citicoline sodium salt) added to conventional treatment (treatment group - TG). Each subject underwent a clinical evaluation and neurophysiological assessment using TMS, pretretament and posttreatment.ResultsA total of thirty participants (mean [SD] age, 68.1 [9.6] years; 11 women [37%]) completed the study. We did not observe significant changes in clinical scores after CDP-choline treatment (all p &gt; 0.05), but we observed a significant improvement in short-interval intracortical inhibition (SAI) (p = 0.003) in the TG group compared to the CG group.ConclusionsThe eight-week treatment with citicoline after acute ischemic stroke may restore intracortical excitability measures, which partially depends on cholinergic transmission. This study extends current knowledge of the application of citicoline in acute ischemic stroke.