bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does Partner Matter in Sexual Activity?
Authors: Dubinskaya A , Jackson F , Shoureshi P , Anger J , Scott V , Eilber K 
Published: July 2022

ABSTRACT

Introduction
A woman's sexual activity is a challenging topic to discuss for both provider and patient. Common questions about sexual activity imply that a woman is having partnered sexual activity with vaginal penetration. Discussion about masturbation or solo sexual experiences tend to be even more uncomfortable and considered a societal taboo. Nevertheless, solo sexual activity is an important part of physical and emotional health.

Objective
The aim of this pilot study is to examine the differences in sexual health, pelvic floor health and overall wellness among women with predominantly partnered sexual activity (PSA) compared to solo sexual activity (SSA).

Methods
This is a prospective pilot study of sexually active women with PSA and SSA age 18 to 99 years who presented for consultation to our Female Pelvic Medicine and Reconstructive Surgery clinic with a pelvic floor complain between May 2021 and August 2021. Participants were given validated questionnaires for 1) sexual health: Female Sexual Function Index (FSFI) and The Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire International Urogynecological Association (IUGA) Revised (PISQ-IR); 2) pelvic floor function: The Pelvic Floor Distress Inventory Questionnaire (PFDI), Genitourinary Pain Index (GUPI), Interstitial cystitis/Painful bladder syndrome (IC/PBS), and 3) overall health: (Self-reported quality of life (SF-12), Patient Health Questionnaire (PHQ-9)). Physical exam and interview on sexual practices were performed. Demographics and health information were recorded. All data were analyzed with descriptive statistics, comparisons were made using Fisher Exact Test and Wilcoxson Rank Sum Tests.

Results
Among 23 women recruited in the pilot study, 13 had PSA and ten women had SSA. Among PSA, six women were also masturbating. Those who were sexually active with a partner were younger (p=0.021), and less likely to have neurologic disorders (p=0.022). No significant differences in demographics, comorbidities, hormonal therapies, pelvic floor disorders, or overall well-being were found between two groups. Women involved in PSA had significantly higher total FSFI score (p=0.035), sexual satisfaction (p=0.012). Interestingly, women with PSA had higher scores on ICSI (p=0.046) and less likely used a vibrator or sex toy ever in their lives (p=0.02).

Conclusions
No significant differences in pelvic health and overall wellness were noted between women with PSA and SSA. Satisfaction from sexual activity is higher among younger women involved in partnered sex, in spite of no significant differences present in desire and orgasm domains. Sexual activity is more than just vaginal penetrative intercourse thus solo sexual practices should be assessed by medical providers especially in older women who might not have available partners.

Disclosure
No
Desire for Solitary Sexual Activity Predicts Discordance Between How Young Women Self-identify and Their Reported Attractions
Authors: Clephane K , Lorenz T 
Published: July 2022

ABSTRACT

Introduction
Introduction: While most clinicians know it is important to tailor sexual healthcare for their sexual minority patients, it can be complicated to assess who identifies with a sexual minority label, as engaging in same-sex behavior or having same-sex attractions does not always lead to an identity as a sexual minority1. Prior work suggests that people who have same-sex attractions or behaviors but identify as heterosexual have different healthcare needs than people who identify as sexual minorities2. Given this, and recent data showing that 1 in 6 young Americans identify with non-heterosexual orientation labels3, it is important to understand what drives discrepancies between young people's attractions and sexual identity label.

Objective
Objective: To determine characteristics that predict discordance between young women's sexual orientation label and reported sexual attractions.

Methods
Methods: 440 young, Midwestern women (age M = 20.6, SD = 1.53) completed a survey battery that assessed their sexual orientation – using both orientation labels and numeric attraction scales – as well as their sexual function (Female Sexual Function Index), sexual desire (Sexual Desire Inventory), sexual sensation seeking (Sexual Sensation Seeking Scale), sexual excitation and inhibition (Sexual Excitation and Inhibition Inventory), and their mental health (Patient Health Questionnaire-9 and Generalized Anxiety Disorder-7). The numeric attraction scale scored participant's attractions to men, women, and non-binary people from 0 – 100. To assess concordance, participant's chosen sexual orientation labels were compared to their numeric attraction scores.

Results
Results: Desire for solitary sexual experiences increased the likelihood of participants indicating identity labels that did not match their reported patterns of attraction (effect size = - .044, z = -2.69, p = .007); these results remained significant when controlling the influence of other sexuality measures (sexual function, sexual sensation seeking, sexual excitation and inhibition). Further, neither depression scores nor anxiety scores were related to the likelihood of identity/attraction concordance.

Conclusions
Conclusions: Desire for solitary sex seems to uniquely predict dissonance between one's stated sexual orientation label and experiencing sexual attraction patterns that may be better encapsulated by another label. A sizeable portion of our sample were in relationships with men, which may be a more salient consideration than attraction patterns when choosing an orientation label. If replicated, these findings suggest that assessing sexual desire patterns may help identify patients whose identity/attraction discordance lead to unique sexual health needs.

Disclosure
Any of the authors act as a consultant, employee or shareholder of an industry for: Good Clean Love
NCX1 coupled with TRPC1 to promote gastric cancer via Ca2+/AKT/β-catenin pathway
Authors: Wan Hanxing , Gao Nannan , Lu Wei , Lu Cheng , Chen Jun , Wang Yimin , Dong Hui 
Published: July 2022

AbstractPlasma membrane Na+/Ca2+ exchanger 1 (NCX1) is a bidirectional ion transporter to operate in Ca2+ entry or exit modes, and TRPC1 is Ca2+-permeable channel. Both NCX1 and TRPC1 play critical roles in maintaining cytosolic free Ca2+ ([Ca2+]cyt) homeostasis in mammalian cells. Although either TRPC1 channel or Ca2+ entry mode of NCX1 is implicated in some tumorigenesis, it has not been explored if a coordination of NCX1 and TRPC1 involves in the pathogenesis of H. pylori-associated human gastric cancer (GC). Here we found the protein expression of NCX1 was significantly enhanced in human GC specimens, which correlated with tumor progression and poor survival in GC patients. TRPC1 and NCX1 were parallelly enhanced, co-localized and bound in human GC cells. By a functional coupling, TRPC1 drives NCX1 to the Ca2+ entry mode, raising [Ca2+]cyt in GC cells. Moreover, CaCl2, H. pylori and their virulence factors all enhanced expressions and activities of NCX1 and TRPC1, and evoked aberrant Ca2+ entry to promote proliferation, migration, and invasion of GC cells through AKT/β-catenin pathway. Tumor growth and metastasis also depended on the enhanced expression of NCX1 in subcutaneously xenografted GC mouse model. Overall, our findings indicate that TRPC1/NCX1 coupling may promote H. pylori-associated GC through the Ca2+/AKT/β-catenin pathway. Since the Ca2+ exit mode and the Ca2+ entry mode of NCX1 play different roles under mostly physiological and pathological conditions respectively, targeting TRPC1/NCX1 coupling could be a novel strategy for selectively blocking Ca2+ entry mode to potentially treat digestive cancer with less side effect.
Perioperative Adriamycin plus ifosfamide vs. gemcitabine plus docetaxel for high-risk soft tissue sarcomas: randomised, phase II/III study JCOG1306
Authors: Tanaka Kazuhiro , Machida Ryunosuke , Kawai Akira , Nakayama Robert , Tsukushi Satoshi , Asanuma Kunihiro , Matsumoto Yoshihiro , Hiraga Hiroaki , Hiraoka Koji , Watanuki Munenori , Yonemoto Tsukasa , Abe Satoshi , Katagiri Hirohisa , Nishida Yoshihiro , Nagano Akihito , Suehara Yoshiyuki , Kawashima Hiroyuki , Kawano Masanori , Morii Takeshi , Hatano Hiroshi , Toguchida Junya , Okuma Tomotake , Takeyama Masanobu , Takenaka Satoshi , Akisue Toshihiro , Furuta Taisuke , Emori Makoto , Hiruma Toru , Outani Hidetatsu , Yamamoto Tetsuji , Kataoka Tomoko , Fukuda Haruhiko , Ozaki Toshifumi , Iwamoto Yukihide 
Published: July 2022

Abstract
Background
This randomised phase II/III trial aimed to determine whether perioperative chemotherapy with gemcitabine plus docetaxel (GD) is non-inferior to the standard Adriamycin plus ifosfamide (AI) in terms of overall survival (OS) in patients with soft tissue sarcoma (STS).

Methods
Patients with localised high-risk STS in the extremities or trunk were randomised to receive AI or GD. The treatments were repeated for three preoperative and two postoperative courses. The primary endpoint was OS.

Results
Among 143 enrolled patients who received AI (70 patients) compared to GD (73 patients), the estimated 3-year OS was 91.4% for AI and 79.2% for GD (hazard ratio 2.55, 95% confidence interval: 0.80–8.14, P = 0.78), exceeding the prespecified non-inferiority margin in the second interim analysis. The estimated 3-year progression-free survival was 79.1% for AI and 59.1% for GD. The most common Grade 3–4 adverse events in the preoperative period were neutropenia (88.4%), anaemia (49.3%), and febrile neutropenia (36.2%) for AI and neutropenia (79.5%) and febrile neutropenia (17.8%) for GD.

Conclusions
Although GD had relatively mild toxicity, the regimen—as administered in this study—should not be considered a standard treatment of perioperative chemotherapy for high-risk STS in the extremities and trunk.

Clinical trial registration
jRCTs031180003.
Single-cell RNA-seq of primary bone marrow neutrophils from female and male adult mice
Authors: Kim Minhoo , Lu Ryan J. , Benayoun Bérénice A. 
Published: July 2022

AbstractWidespread sex-dimorphism is observed in the mammalian immune system. Consistently, studies have reported sex differences in the transcriptome of immune cells at the bulk level, including neutrophils. Neutrophils are the most abundant cell type in human blood, and they are key components of the innate immune system as they form a first line of defense against pathogens. Neutrophils are produced in the bone marrow, and differentiation and maturation produce distinct neutrophil subpopulations. Thus, single-cell resolution studies are crucial to decipher the biological significance of neutrophil heterogeneity. However, since neutrophils are very RNA-poor, single-cell profiling of these cells has been technically challenging. Here, we generated a single-cell RNA-seq dataset of primary neutrophils from adult female and male mouse bone marrow. After stringent quality control, we found that previously characterized neutrophil subpopulations can be detected in both sexes. Additionally, we confirmed that canonical sex-linked markers are differentially expressed between female and male cells across neutrophil subpopulations. This dataset provides a groundwork for comparative studies on the lifelong transcriptional sexual dimorphism of neutrophils.
Synthesis and physicochemical characterization of bovine lactoferrin supersaturated complex with iron (III) ions
Authors: Pryshchepa Oleksandra , Rafińska Katarzyna , Gołębiowski Adrian , Sugajski Mateusz , Sagandykova Gulyaim , Madajski Piotr , Buszewski Bogusław , Pomastowski Paweł 
Published: July 2022

AbstractThe aim of the study was to investigate the process of Fe3+ binding to bLTF. Moreover, the physicochemical characterization of the respective supersaturated complex was studied. The knowledge should be important for the description of processes that may take place in dairy products fortified with iron. Additionally, the synthesized complex can be utilized as a dietary supplement for the treatment of iron deficiency anemia (IDA). Finally, it was shown that formation of supersaturated iron-protein structures which include LTF often accompanies development of neurodegenerative diseases such as Alzheimer or Parkinson. Thus, the study can reveal some aspects of its pathogenesis process. The methodology of the investigation comprised the utilization of batch sorption study and applying Freundlich and Langmuir models. The complex also was characterized by numerous techniques: spectrometric (ICP-MS), spectroscopic (UV–Vis, ATR-FTIR), electron microscopy (TEM–EDX), SDS-PAGE. Based on obtained results the potential mechanisms of iron interaction with protein were described. Moreover, the molecular docking was applied to visualize possible metal binding sites. The respective complex contains ≈ 33.0 mg/g of iron which is nearly 50 Fe3+ per one protein molecule. The cytotoxicity of the obtained complex was evaluated by MTT reduction and LDH release assays on Caco-2 and nL929 cell lines.
Psilocybin reduces low frequency oscillatory power and neuronal phase-locking in the anterior cingulate cortex of awake rodents
Authors: Golden Caroline T. , Chadderton Paul 
Published: July 2022

AbstractPsilocybin is a hallucinogenic compound that is showing promise in the ability to treat neurological conditions such as depression and post-traumatic stress disorder. There have been several investigations into the neural correlates of psilocybin administration using non-invasive methods, however, there has yet to be an invasive study of the mechanism of action in awake rodents. Using multi-unit extracellular recordings, we recorded local field potential and spiking activity from populations of neurons in the anterior cingulate cortex of awake mice during the administration of psilocybin (2 mg/kg). The power of low frequency bands in the local field potential was found to significantly decrease in response to psilocybin administration, whilst gamma band activity trended towards an increase. The population firing rate was found to increase overall, with just under half of individual neurons showing a significant increase. Psilocybin significantly decreased the level of phase modulation of cells with each neural frequency band except high-gamma oscillations, consistent with a desynchronization of cortical populations. Furthermore, bursting behavior was altered in a subset of cells, with both positive and negative changes in the rate of bursting. Neurons that increased their burst firing following psilocybin administration were highly likely to transition from a phase-modulated to a phase unmodulated state. Taken together, psilocybin reduces low frequency oscillatory power, increases overall firing rates and desynchronizes local neural activity. These findings are consistent with dissolution of the default mode network under psilocybin, and may be indicative of disruption of top-down processing in the acute psychedelic state.
Invasive brown treesnakes (Boiga irregularis) move short distances and have small activity areas in a high prey environment
Authors: Boback Scott M. , Nafus Melia G. , Yackel Adams Amy A. , Reed Robert N. 
Published: July 2022

AbstractAnimal movements reflect temporal and spatial availability of resources as well as when, where, and how individuals access such resources. To test these relationships for a predatory reptile, we quantified the effects of prey abundance on the spatial ecology of invasive brown treesnakes (Boiga irregularis) on Guam. Five months after toxicant-mediated suppression of a brown treesnake population, we simultaneously used visual encounter surveys to generate relative rodent abundance and radiotelemetry of snakes to document movements of surviving snakes. After snake suppression, encounter rates for small mammals increased 22-fold and brown treesnakes had smaller mean daily movement distances (24 ± 13 m/day, $$\overline{x }$$

x
¯

± SD) and activity areas (5.47 ± 5 ha) than all previous observations. Additionally, snakes frequenting forest edges, where our small mammal encounters were the highest, had smaller mean daily movement distances and three-dimensional activity volumes compared to those within the forest interior. Collectively, these results suggest that reduced movements by snakes were in part a response to increased prey availability. The impact of prey availability on snake movement may be a management consideration when attempting to control cryptic invasive species using tools that rely on movement of the target species to be effective.
Optical characterization of deuterated silicon-rich nitride waveguides
Authors: Chia Xavier X. , Chen George F. R. , Cao Yanmei , Xing Peng , Gao Hongwei , Ng Doris K. T. , Tan Dawn T. H. 
Published: July 2022

AbstractChemical vapor deposition-based growth techniques allow flexible design of complementary metal-oxide semiconductor (CMOS) compatible materials. Here, we report the deuterated silicon-rich nitride films grown using plasma-enhanced chemical vapor deposition. The linear and nonlinear properties of the films are characterized, and we experimentally confirm that the silicon-rich nitride films grown with SiD4 eliminates Si–H and N–H related absorption. The performance of identical waveguides for films grown with SiH4 and SiD4 are compared demonstrating a 2 dB/cm improvement in line with that observed in literature. Waveguides fabricated on the SRN:D film are further shown to possess a nonlinear parameter of 95 W−1 m−1, with the film exhibiting a linear and nonlinear refractive index of 2.46 and 9.8 $$\times$$
×
10–18 m2W−1 respectively.
Harnessing the ubiquitin code to respond to environmental cues
Authors: Orosa-Puente Beatriz , Spoel Steven H. 
Published: July 2022

Abstract
Ubiquitination is an essential post-translational signal that allows cells to adapt and respond to environmental stimuli. Substrate modifications range from a single ubiquitin molecule to complex polyubiquitin chains, where diverse chain topologies constitute a code that is utilized to modify the functions of proteins in numerous cellular signalling pathways. Diverse ubiquitin chain topologies are generated by linking the C-terminus of ubiquitin to one of seven lysine residues or the N-terminal methionine 1 residue of the preceding ubiquitin. Cooperative action between a large array of E2 conjugating and E3 ligase enzymes supports the formation of not only homotypic ubiquitin chains but also heterotypic mixed or branched chains. This complex array of chain topologies is recognized by proteins containing linkage-specific ubiquitin-binding domains and regulates numerous cellular pathways. Although many functions of the ubiquitin code in plants remain unknown, recent work suggests that specific chain topologies are associated with particular molecular processes. Deciphering the ubiquitin code and how plants utilize it to cope with the changing environment is essential to understand the regulatory mechanisms that underpin myriad stress responses and establishment of environmental tolerance.
Neural networks and PCA coefficients to identify and correct aberrations in adaptive optics
Authors: Terreri A. , Pedichini F. , Del Moro D. , Li Causi G. , Stangalini M. , Mattioli M. , Piazzesi R. 
Published: July 2022

Context. Static and quasi-static aberrations represent a great limit for high-contrast imaging in large telescopes. Among them the most important ones are all the aberrations not corrected by the adaptive optics (AO) system, which are called non-common path aberrations (NCPA). Several techniques have been proposed to mitigate it. The typical approach is to set an offset on the AO system with exactly the opposite sign of the NCPA in order to correct for the aberrations introduced by all the optical components downstream the wave-front sensor (WFS) up to the science camera. An estimate of the NCPA can be obtained with a trial-and-error approach or by more sophisticated techniques of focal-plane wave-front sensing.
Aims. In all cases, a fast procedure is desirable to limit the telescope downtime and to repeat, if needed, the correction procedure to cope with the temporal variation of the NCPA. Very recently, new approaches based on neural networks (NNs) have also been proposed as an alternative.
Methods. In this work, through simulated images, we test the application of a supervised NN for the mitigation of NCPAs in high-contrast imaging at visible wavelengths and, in particular, we investigate the possibility of applying this method to fast imagers such as SHARK-VIS, the forthcoming visible-band high-contrast imager for the Large Binocular Telescope (LBT).
Results. Preliminary results show a measurement accuracy of the NCPA of 2 nm root mean square (RMS) for each sensed Zernike mode in turbulence-free conditions, and 5 nm RMS per mode when the residual turbulence has a wave-front error (WFE) of approximately 42.5 nm RMS, a typical value during LBT AO system calibration. This measurement is sufficient to guarantee that, after correction, NCPA residuals in the system are negligible compared to the typical WFE > 100 nm RMS of the best AO systems at large telescopes.
Conclusions. Our simulations show this method is robust even in the presence of turbulence-induced aberrations that are not labelled in the training phase of the NN. The method could thus be used in a real-world setting by offloading a corrective static offset to the AO system of a telescope to mitigate the NCPA.