bluuebunny/crossref_metadata_2025_split huggingface dataset:
The circulating miRNA-34a, miRNA -143 and miRNA -212 are promising biomarkers in ovarian cancer
Authors: Kheirandish Shahnaz , Zarezade Zeynab , Yavari Mahdie , Sheikhha Mohammad Hasan , Kalantar Seyyed Mahdi , Sahami-Fard Mohammad Hossein , Hasani Shahrzad Sheikh , Ghasemi Nasrin , Jahantigh Hamid Reza 
Published: March 2022

Abstract
Objective
Ovarian cancer is one of the most common gynaecological malignancies among females worldwide. Early diagnostic of ovarian cancer is challenging, and miRNAs could serve as a potential biomarker for early detection of ovarian cancer regarding easy to detect. The present study investigated the expression profile of miRNA-34a, miRNA-143, miRNA-212, Sox4, BCL-2, and E2f5 in the serum of participants to find new biomarkers for early diagnosis of ovarian cancer.
Materials and Methods
Five milliliters of whole blood were collected from each patient (n = 30) and control (n = 30), and total RNA was extracted using Ambion™ TRIzol™ Reagent. Real-time PCR methods evaluated the expression of targeted miRNAs and their targeted genes.
Result
The Disease status of all ovarian cancer patients were classified as FIGO stage III (21) and (IV 9) according to imaging studies and surgical pathological findings. Our results showed that expression levels of miRNA-34a (p < 0.0001), miRNA-143 (p = 0.028), and miRNA-212 (p < 0.0001) were significantly decreased in patients with ovarian cancer compared to controls and expression levels of Sox4(p < 0.0001), BCL-2(p < 0.0001) and E2f5(p < 0.0001) were significantly increased in patients with ovarian cancer compared to controls. In addition, Receiver Operating Characteristic (ROC) curve revealed that the expression profile of miRNA-34a (AUC = 0.82; P < 0.0001), miRNA-143 (AUC = 0.66; P = 0.026) and miRNA-212 (AUC = 0.78; P = 0.0001) could be used as potential biomarker for discriminating patients with ovarian cancer.
Conclusion
In conclusion, our data showed that the expression profile of miRNA-34a, miRNA-143, and miRNA-212 could act as a potential biomarker for diagnosing ovarian cancer patients.
Improved Antibacterial Activity by Incorporation of Silver Sulfadiazine on Nanoporous Cu-BTC Metal-Organic-Framework
Authors: Soltani Sajjad , Akhbari Kamran , Phuruangrat Anukorn 
Published: March 2022

Abstract
Silver sulfadiazine (SSD) as an antibacterial agent is widely used in the topical treatment of wound infections. Metal-organic frameworks (MOFs) have also been considered in the field of drug delivery in recent years due to their attractive properties. In this work, in order to increase the antibacterial efficiency, the Cu-BTC MOF was used as a platform for the incorporation of silver sulfadiazine. Physical characterization of compounds was performed using FT-IR, PXRD, SEM, TEM, EDX-mapping, N2 adsorption-desorption and TGA techniques. The performance of the compounds against Staphylococcus aureus and Escherichia coli bacterium was assessed by agar well diffusion and plate colony count bioassays. SSD loading on the Cu-BTC MOF resulted in a synergistic effect in antibacterial activity.
Expression analysis of oncogene transcript in human Aberrant Crypt foci, in comparison to the normal colonic mucosa and colorectal carcinoma from formalin-fixed paraffin-embedded tissue samples: A Pilot Study
Authors: Sulaiman Mohamed , Sarangi Jayati , Ahmed Aijaz , Ghosh Shouriyo , Mehra Lailta , Gupta Brijnandan , Upadhyay Ashish Datt , Pal Sujoy , Dash Nihar R , Ahuja Vineet , Yadav Rajni , Sharma Atul , Deo SVS , Gupta Siddhartha Datta , DAS PRASENJIT 
Published: March 2022

Abstract
Purpose: Aberrant Crypt Foci (ACF) are microscopic preneoplastic lesions in human colon detectable by magnified chromoendoscopy. Our aim was to analyze the expression of a chosen gene transcript in harvested colonic ACF, corresponding colorectal carcinomas (CRC) and normal mucosa. Methods: A total of 35 cases having ACF >4 in number/ 4 mm2 colonic mucosa were selected from 302 fresh colectomy samples screened along with the corresponding CRC (35) and normal colonic mucosal shavings (20). Gene expression analysis was performed by reverse transcriptase-polymerase chain reaction in these 3-groups. The gene expression data were correlated with histological and topographical ACF types, lymph node metastasis, site, size, and stage of tumors. Results: The KRAS, CDKN1A, CDKN2A, MLH1, VEGFA, and CCL5 gene expressions were significantly altered in ACF compared to controls (p <0.01), while the genes CDNK2A, PTEN (p 0.01), and SMAD4 (p 0.05) were significantly altered in CRC than in controls. The gene expression profile of the mucosal ACF and corresponding CRC foci were similar. No definitive correlation was found between topographic and histological types of ACF and gene expressions. Up-regulation of IGF1 and EGFR genes in ACF were associated with higher lymph node metastases and larger tumor sizes respectively, while down-regulation of RB1 and Bcl2 genes were associated with smaller tumor size. Conclusions The molecular pathogenesis of ACF is as complex as that like advanced CRC foci. Our observation is fascinating as it brings forth the complex pathogenesis of these early mucosa lesions and defies sequential molecular accumulation hypothesis.
The Wellbeing In Place Perceptions Scale: Structure, Validity, Reliability and Correlates during COVID times.
Authors: Corcoran Rhiannon , Gomez Katalin Ujhelyi , Simpson Grace , Goodall Mark , Bennett Kate M , Gabbay Mark , Wilson Tim , Obe Dorcas Akeju , Pennington Andy , Bagnall Anne-Marie , South Jane 
Published: March 2022

Abstract

The influence of place-based factors on the physical and mental health of residents is well established and acknowledged within the population health approach to addressing health and wellbeing inequalities. The COVID-19 pandemic brought to the fore the issues that global communities face. The current UK policy context of ‘levelling up’ represents these concerns and the need to address them. This research examines perceptions of community wellbeing and its determinants as collected within a city region of the North West Coast of England during COVID restrictions between June and August 2020. The paper aims to establish the structure, construct validity and reliability of a new measure of community wellbeing - the Wellbeing in Place Perceptions Scale. Further, it aims to examine how this measure of community wellbeing correlated with symptoms of common mental health as reported by residents of this relatively disadvantaged city region during this unprecedented time. Results indicate that the WIPPS has a reliable and valid structure, correlating significantly with another widely used measure of sense of community and with the Index of Multiple Deprivation. Its relationship to self-reported common mental distress is also clear. Though in need of replication and longitudinal testing, the findings reported here on this new measure remind us that individual and place-based factors combine to influence wellbeing and that community needs to have an increasingly influential role to sustainably prevent future mental health challenges.
Identification of biological processes and signaling pathways in bone tissues from mice with diabetes
Authors: Wang Mengyao , Wang Mengshan , Wang Xiu-ying 
Published: March 2022

Abstract
Diabetic patients have a higher fracture risk than expected by their bone mineral density (BMD), but the mechanism is still unknown. Here, our objective is to identify the functional molecules and signaling pathways by analyzing the RNA-seq data. The GSE189112 was created by the BGISEQ-500 (Mus musculus). The KEGG and GO analyses showed the generation of precursor metabolites and energy and energy derivation by oxidation of organic compounds are the major biological processes in the monocytes of diabetes mice. Moreover, we also identified the ten interacting molecules including RPS27A, RPS3, UBC, GNB2L1, RPS18, RPL23A, RPL5, RPLP0, RPS4X, RPL13. Therefore, this study may help understand diabetes-induced bone dysfunction.
A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection
Authors: Liu Yuan , Li Taotao , Yang Gaojian , Deng Yan , Mou Xianbo , He Nongyue 
Published: March 2022

Abstract
In this work, a simple gold nanoparticles (AuNPs) based colorimetric biosensor was developed for chlorpyrifos (Chl) detection using an aptamer as the capture probe. The Chl-aptamer with low dissociation constant (Kd) of 58.59 ± 6.08 (nmol/L) was selected by ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for AuNPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately, thus a self-aggregation of AuNPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the LOD as low as 14.46 nmol/L. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, the excellent recoveries with acceptable RSD values below 5% demonstrated that the method can be considered as a promising tool for simple, rapid Chl detection.
A new approach in qualitative zoning of groundwater to assessment suitable irrigation water using fuzzy logic spatial modeling via GIS
Authors: Tafreshi Amin Mohebbi , Tafreshi Ghazaleh Mohebbi 
Published: March 2022

Abstract
Water quality must be tested before it can be used for agricultural purposes since difficulties might arise in terms of increased soil salinity, decreased soil permeability, and decreased water absorption by plant roots; leading to reduced agricultural productivity. The aim of this study was to assessment suitable irrigation water based on a new approach in Asalem region, Iran. For reduce the uncertainty, fuzzy logic spatial modeling via GIS was applied. To receive these aims, four stages were performed. In stage 1, we calculate the values of nine conventional and effective parameters used for agricultural water quality classification upon analyzing the statistical quality data regarding various ions existing in 15 sample wells dug in 2015. In stage 2, interpolate these parameters by kriging method via ArcGIS software. In stage 3, parameter standardization with fuzzy membership function were done. And in stage 4, for Aggregation parameters, several fuzzy overlay operations were used. Finally, to identify the best operation, the correlations between the fuzzy membership and operation maps were used. Results showed that the "GAMMA 0.9" with six high correlation above 80%, is the best overly operation in our study area. According to the best operation map, only west and northwest parts of the study area have "good" to "excellent" groundwater quality for irrigation water and in other parts, the quality is within "poor" to "fair".
A comparative study of percutaneous endoscopic interlaminar discectomy and transforaminal discectomy for L5-S1 calcified lumbar disc herniation
Authors: Cheng Yuanpei , Cheng Xiaokang , Wu Han 
Published: March 2022

Abstract
Background: Percutaneous endoscopic lumbar discectomy (PELD) is a relatively safe and effective minimally invasive surgery in the treatment of calcified lumbar disc herniation (CLDH). However, studies on percutaneous endoscopic interlaminar discectomy (PEID) and percutaneous endoscopic transforaminal discectomy (PETD) for CLDH have rarely been reported. This research aimed to compare the clinical efficacy of PEID and PETD for L5-S1 CLDH.Methods: We retrospectively analyzed 54 consecutive patients with L5-S1 CLDH treated with PELD at our institution from August 2016 to August 2020. Patients were divided into PEID group (n = 28) and PETD (n = 26) group according to the surgical methods. The demographic characteristics and surgical results of the two groups were compared. Clinical outcomes were estimated by the visual analog scale (VAS) for leg pain, Oswestry disability index (ODI) and modified MacNab criteria.Results: All patients were successfully operated on by PEID or PETD. No significant differences in the demographic characteristics, intraoperative blood loss, postoperative hospital stay and complication rate were noted between the PEID and PETD groups. The excellent and good rates in the PEID group were similar to those in the PETD group (89.29% vs 88.46%, P = 1.000), whereas the PEID group exhibited superior results for operative time (min) (64.61 ± 5.60 vs 85.58 ± 8.52, P < 0.001) and fluoroscopy times (n) (2.93 ± 0.90 vs 13.35 ± 2.30, P < 0.001) compared with the PETD group.Conclusions: PEID has achieved good clinical efficacy as PETD for L5-S1 CLDH. Compared with PETD, PEID has the advantages of shorter operative time and a reduced number of fluoroscopy times in the treatment of CLDH.
Current tobacco use and susceptibility to using tobacco among non-users of tobacco: a cross-sectional study among school-going adolescents in Sierra Leone.
Authors: James Peter Bai , Kabba John Alimamy , Bah Abdulai Jawo , Idriss Ayesha , Kitchen Chenai , Conteh Eugene B , Lahai Michael , Dalinjong Philip Ayizem 
Published: March 2022

Abstract
IntroductionTobacco use is a global health threat associated with a high disease burden and deaths. Studies concerning current tobacco use and susceptibility to using tobacco products among adolescents who are potential adult tobacco users in Sierra Leone have not been explored. We aimed to estimate the prevalence and correlates of current tobacco use and tobacco non-users susceptibility to using tobacco amongst Sierra Leonean high-school students.MethodWe used data obtained from the 2017 Sierra Leone Global Youth Tobacco Survey (GYTS), which presented information collected from 6680 students aged 11-17years nationwide. Gender-based correlates of current use and susceptibility to using tobacco among non-tobacco users were determined by complex sample logistic regression analyses. Adjusted odds ratios (AOR) and respective 95% confidence intervals (CIs) were reported. p-values less than 0.005 were considered significant.ResultsThe prevalence of current tobacco use among high school adolescents in Sierra Leone was 24.6% and was higher in males (27.9%) than in females (18.6%). Male [AOR=1.50 (95%CI: 1.18-1.91)], parental smoking [AOR=1.73(95%CI:1.32-2.26)] exposure to household secondhand smoke [AOR=1.82(95%CI:1.27-2.62)], having peers who smoke [AOR=2.24(1.51-3.31)] were more likely to be currently using tobacco. On the other hand, adolescents exposed to antismoking media messages were less likely to be current tobacco users [AOR=0.67(95%CI: 0.52-0.85)]. The overall tobacco non-users susceptibility to using tobacco among adolescents in Sierra Leone was 18.2% [male (18.0%), female (18.5%)]. Exposure to tobacco promotion [AOR =1.50(95%CI:1.06-2.10)] and non-exposure to antismoking education [AOR =1.39(95%CI:1.05-1.86)] were significantly associated with tobacco non-users’ susceptibility to using tobacco.ConclusionOur study suggests that one-in-four school-going adolescents currently use tobacco, with nearly one in five non-users susceptible to using tobacco. Given the high prevalence of tobacco product use among Sierra Leonean adults, our findings highlight the need for policies and interventions to prevent tobacco use behaviour among adolescents aimed at averting tobacco use in adulthood.
Risk management during the Covid-19 crisis: business responses in the Polish water utilities
Authors: Chudziński Paweł , Cyfert Szymon , Dyduch Wojciech , Zastempowski Maciej 
Published: March 2022

Abstract
Water utilities are an essential service that helps protect public health during crises. The Covid-19 pandemic revealed that crisis preparedness is a crucial capability that water utilities must possess. The purpose of this paper is to identify managerial actions and responses that were undertaken by water utility managers in order to reduce the risk related to the first economic lockdown caused by the unexpected Covid-19 crisis. As water utilities should learn from Covid-19 so as to strengthen their future risk preparedness, the paper offers some theoretical underpinnings on risk management. As a result of literature analysis, we focus on the risk management framework that distinguishes five types of risk. The survey was carried out among 116 waterworks in Poland in April 2020. The results indicate the importance of minimising liquidity risk and supply chain risk, which is relevant to the adopted theoretical framework. The findings also highlight the importance of a category that was not originally included in the research model – that is human resource risk, an area that requires managerial attention in the water utility sector. The results could also provide useful pointers for other water utilities, especially those operating in the same or similar legislative regime.
Discussion on the Structural Modification and Anti-tumor Activity of
Flavonoids
Authors: Zhou Zhu-Gui , Li Dong-Dong , Chen Ying , Chen Xi , Man Ruo-Jun 
Published: March 2022

Abstract:
Flavonoids are secondary metabolites of plants. In general，most flavonoids are combined with glucosides and have extremely complex molecular structures. In the nature，these flavonoids have a variety of biological activities，such as anti-oxidation，anti-virus，anti-tumor，scavenging free radicals and so on，however，due to poor solubility and stability of flavonoids，their bioavailability is limited. The method of drug design is used to modify the structure of flavonoids to give them special functions. At present, flavonoids have broad application prospects in the treatment of tumors. It has inhibited the proliferation, migration, invasion, angiogenesis and multi-drug resistance of tumors and has become a research hotspot.
Application of Herbal Plants in Organic Poultry Nutrition and Production
Authors: Sun Wenli , Shahrajabian Mohamad Hesam , Cheng Qi 
Published: March 2022

Background:
Medicinal plants and natural feed additives are the most important
alternatives in animal production, especially in broiler production.

Objective:
In this mini-review article, some important medicinal herbs and plants with positive
effects on organic broiler production have been highlighted.

Methods:
A literature search was conducted using Science Direct, Google, Google Scholar,
Springer, Medline, and PubMed.

Results:
Medicinal plants such as ginger, ginkgo, thyme, ponderosa pine, soybean, forsythia,
peppermint, Chinese star anise, astragalus, mistletoe, schisandra, cumin, capsicum, garlic, hooker
chives, artichoke, Borreria latifolia, zataria, pomegranate, turmeric, lingzhi, Lippia javanica,
neem, oriental chaff flower, mulberry leaf, goji berry, Aloe vera, pumpkin, grape, common
nettle, marigold, coriandrum, Citrus sinensis, Alisma canaliculatum, Persian hogweed,
Eucommiaulmoides, bamboo leaf extract, rosemary, Morina citrifolia, chestnut, green tea, wild
mint, clove, sumac, satureja, ashwagandha, Lonicera japonica, Acacia, liquorice, Artemisia
annua, milk thistle, cinnamon, black cumin, etc., have positive effects on organic broiler
production.

Conclusion:
Herbal medicines lead to increased body weight due to a higher feed intake and a
higher feed conversion ratio. They improve the antioxidant activity of broiler chickens based on
their phenolic compound contents. The combination of medicinal herbal additives also has a
positive impact on broiler production. Reducing hormones, growth promoters, and antibiotics, as
well as addressing appropriate organic feed management and higher production, should be
organized in organic broiler production with the use of herbal plants.
Knowledge and Awareness of School Teachers Regarding Emergency Management of Tooth Avulsion in the Kingdom of Saudi Arabia: A Cross-Sectional Study
Authors: Almulhim Basim 
Published: March 2022

Aim:This study aimed to assess the general knowledge and awareness of school teachers regarding the first aid emergency dental management of tooth avulsion in the kingdom of Saudi Arabia.Materials and Methods:A self-administered, anonymous, close-ended structured questionnaire was given to school teachers in Saudi Arabia. It included information regarding gender, knowledge, and awareness [three parts: basic demographic characteristics focusing on the gender (part one), questions regarding knowledge of school teachers toward emergency management of tooth avulsion (part two), and questions regarding awareness of school teachers toward emergency management of tooth avulsion (part three)].Results:A total of 615 school teachers (58% males and 42% females) completed the questionnaire. Approximately 54.3% of the school teachers preferred to immediately refer the child to the dentist if trauma affects permanent teeth (p < 0.05), and only 2.1% opined regarding reposition of the tooth in a normal position. Another 22.9% responded that they would scrub the tooth to clean the dirt and rinse with water, while 19.7% reported that they would keep the tooth in a wet handkerchief until they reach the dentist.Conclusion:The knowledge and awareness of schoolteachers regarding the management of avulsed teeth are insufficient; thus, education programs on traumatic dental injuries are essential.
A Reusable Multiplayer Game for Promoting Active School Transport: Development Study
Authors: Laine Teemu H , Duong Nhi , Lindvall Helena , Oyelere Solomon Sunday , Rutberg Stina , Lindqvist Anna-Karin 
Published: March 2022

Background
Most children and adolescents in Sweden do not meet the recommended daily physical activity levels of the World Health Organization. Active school transport (AST) and gamification are potential methods for increasing children’s daily physical activity. We previously developed a game named Tic-Tac-Training for promoting active transport at workplaces; however, the game has not been applied to AST.

Objective
The objectives of this study are to investigate how Tic-Tac-Training functions to promote AST among schoolchildren in northern Sweden, improve the game to be more suitable for schoolchildren, and construct a road map for future development based on children’s ideas.

Methods
First, we developed Tic-Tac-Training using the Scrum agile software development method. Second, we conducted a questionnaire-based formative evaluation of the game with schoolchildren (n=16; 9/16, 56% male; 6/16, 38% female; and 1/16, 6% other aged 11-12 years) in Luleå, Sweden. Third, we conducted focus group interviews with 33 children (13/33, 39% male and 20/33, 61% female aged 12-13 years) to gather ideas for gamifying AST. We mapped the interview results to the Octalysis gamification framework and established a road map for future development.

Results
The formative evaluation revealed several issues, including a lack of interesting game features, lack of support for continuous engagement, disliked competitive features, and lack of incentives for discourse and participation. New features such as rewards, collectibles, and levels were implemented based on the results. The focus group interviews revealed additional ideas for gamifying AST, such as using avatars, in-game currency and trading, and context-sensitive tasks.

Conclusions
The results have several potential impacts on how reusable, gamified AST interventions can be developed and what kind of gamification elements schoolchildren in northern Sweden wish to see. These results can interest game researchers and teachers who wish to apply gamification in school contexts. Finally, we aim to continue developing the game based on the road map.