bluuebunny/crossref_metadata_2025_split huggingface dataset:
KANK4 Promotes Arteriogenesis by Potentiating VEGFR2 Signaling in a TALIN-1–Dependent Manner
Authors: Zhang Chonghe , He Hao , Dai Jianing , Li Yunxia , He Jing , Yang Wu , Dai Jialin , Han Feng , Kong Wenyan , Wang Xiaohong , Zheng Xiangjian , Zhou Jing , Pan Weijun , Chen Zhongwen , Singhal Mahak , Zhang Yaoyang , Guo Feng , Hu Junhao 
Published: April 2022

Background:
Arteriogenesis plays a critical role in maintaining adequate tissue blood supply and is related to a favorable prognosis in arterial occlusive diseases. Strategies aimed at promoting arteriogenesis have thus far not been successful because the factors involved in arteriogenesis remain incompletely understood. Previous studies suggest that evolutionarily conserved KANK4 (KN motif and ankyrin repeat domain-containing proteins 4) might involve in vertebrate vessel development. However, how the KANK4 regulates vessel function remains unknown. We aim to determine the role of endothelial cell-specifically expressed KANK4 in arteriogenesis.

Methods:

The role of KANK4 in regulating arteriogenesis was evaluated using

Kank4
−/−

and

KANK4
iECOE

mice. Molecular mechanisms underlying KANK4-potentiated arteriogenesis were investigated by employing RNA transcriptomic profiling and mass spectrometry analysis.

Results:

By analyzing
Kank4-EGFP
reporter mice, we showed that KANK4 was specifically expressed in endothelial cells. In particular, KANK4 displayed a dynamic expression pattern from being ubiquitously expressed in all endothelial cells of the developing vasculature to being explicitly expressed in the endothelial cells of arterioles and arteries in matured vessels. In vitro microfluidic chip-based vascular morphology analysis and in vivo hindlimb ischemia assays using

Kank4
−/−

and

KANK4
iECOE

mice demonstrated that deletion of KANK4 impaired collateral artery growth and the recovery of blood perfusion, whereas KANK4 overexpression leads to increased vessel caliber and blood perfusion. Bulk RNA sequencing and Co-immunoprecipitation/mass spectrometry (Co-IP/MS) analysis identified that KANK4 promoted EC proliferation and collateral artery remodeling through coupling VEGFR2 (vascular endothelial growth factor receptor 2) to TALIN-1, which augmented the activation of the VEGFR2 signaling cascade.

Conclusions:
This study reveals a novel role for KANK4 in arteriogenesis in response to ischemia. KANK4 links VEGFR2 to TALIN-1, resulting in enhanced VEGFR2 activation and increased EC proliferation, highlighting that KANK4 is a potential therapeutic target for promoting arteriogenesis for arterial occlusive diseases.
Cardiovascular Risk Assessment Using Artificial Intelligence-Enabled Event Adjudication and Hematologic Predictors
Authors: Truslow James G. , Goto Shinichi , Homilius Max , Mow Christopher , Higgins John M. , MacRae Calum A. , Deo Rahul C. 
Published: April 2022

Background:
Researchers routinely evaluate novel biomarkers for incorporation into clinical risk models, weighing tradeoffs between cost, availability, and ease of deployment. For risk assessment in population health initiatives, ideal inputs would be those already available for most patients. We hypothesized that common hematologic markers (eg, hematocrit), available in an outpatient complete blood count without differential, would be useful to develop risk models for cardiovascular events.

Methods:
We developed Cox proportional hazards models for predicting heart attack, ischemic stroke, heart failure hospitalization, revascularization, and all-cause mortality. For predictors, we used 10 hematologic indices (eg, hematocrit) from routine laboratory measurements, collected March 2016 to May 2017 along with demographic data and diagnostic codes. As outcomes, we used neural network-based automated event adjudication of 1 028 294 discharge summaries. We trained models on 23 238 patients from one hospital in Boston and evaluated them on 29 671 patients from a second one. We assessed calibration using Brier score and discrimination using Harrell’s concordance index. In addition, to determine the utility of high-dimensional interactions, we compared our proportional hazards models to random survival forest models.

Results:
Event rates in our cohort ranged from 0.0067 to 0.075 per person-year. Models using only hematology indices had concordance index ranging from 0.60 to 0.80 on an external validation set and showed the best discrimination when predicting heart failure (0.80 [95% CI, 0.79–0.82]) and all-cause mortality (0.78 [0.77–0.80]). Compared with models trained only on demographic data and diagnostic codes, models that also used hematology indices had better discrimination and calibration. The concordance index of the resulting models ranged from 0.75 to 0.85 and the improvement in concordance index ranged up to 0.072. Random survival forests had minimal improvement over proportional hazards models.

Conclusions:
We conclude that low-cost, ubiquitous inputs, if biologically informative, can provide population-level readouts of risk.
Strengthening Amplitude and Impact of the Pacific Meridional Mode on ENSO in the Warming Climate Depicted by CMIP6 Models
Authors: Fan Hanjie , Yang Song , Wang Chunzai , Wu Yuting , Zhang Guangli 
Published: April 2022

Abstract
The Pacific meridional mode (PMM) has been suggested to play an important role in modulating the development of El Niño–Southern Oscillation (ENSO). In this study, we examine the projected changes in the PMM and its impact on ENSO under greenhouse gas forcing using the models of phase 6 of the Coupled Model Intercomparison Project. These models can properly reproduce the characteristics of PMM patterns but reveal discrepant PMM–ENSO relationships owing to different wind–evaporation–sea surface temperature (SST) (WES) feedback efficiency and different magnitude of atmospheric convection response to SST anomalies. We select the models that show good performance in simulating the PMM and its impact on ENSO for investigation of future projections. Results show potential increases in both PMM amplitude and its impact on ENSO under the shared socioeconomic pathway (SSP) 585 (SSP585) warming scenario with great intermodel consensus. Diagnosis of the WES feedback indicates increasing sensitivity of latent heat flux to zonal wind speed in a warming climate, which seems to be the main reason for the projected strengthening PMM and its impact on ENSO. In addition, a slightly intensified response of atmospheric convection to SST anomalies in the subtropical Pacific may partially contribute to a stronger PMM–ENSO relationship. The results from this study highlight the increasing importance of the PMM for ENSO development, which calls for more attention to be paid to the PMM for ENSO prediction.

Significance Statement
Variability of the sea surface temperature in the equatorial Pacific related to El Niño–Southern Oscillation (ENSO) can exert a great impact on global climate. The development of ENSO is partially modulated by the dominant mode of ocean–atmospheric variation in the subtropical North Pacific, namely, the Pacific meridional mode (PMM). This study is aimed to understand the change in the amplitude of the PMM and its impact on ENSO due to climate change. Multimodel projections suggest that the PMM will likely become stronger and exert a greater impact on ENSO since the future warmer climate is favorable for the growth of the PMM. These results call for more attention to be paid to the PMM for ENSO prediction.
Second Baroclinic Mode Rossby Waves in the South Indian Ocean
Authors: Nagura Motoki , Osafune Satoshi 
Published: April 2022

Abstract
Many previous studies of midlatitude Rossby waves have examined satellite altimetry data, which reflect variability near the surface above the pycnocline. Argo float observations provide hydrographic data in the upper 2000 m, which likely monitor subsurface variability below the pycnocline. This study examines the variability in meridional velocity at midlatitudes and investigates Rossby waves in the southern Indian Ocean using an ocean reanalysis generated by a 4DVAR method. The results show two modes of variability. One is trapped near the surface and propagates to the west at a phase speed close to that of first baroclinic mode Rossby waves. This mode is representative of variability detected by satellite altimetry. The other mode has a local peak in amplitude at ∼600-m depth and propagates to the west at a phase speed 3 times slower than the first baroclinic mode. Such slowly propagating signals are observed globally, but they are largest in amplitude in the southern Indian Ocean and consistent in phase speed with the second baroclinic mode. Results from numerical experiments using an OGCM show that zonal winds in the tropical Pacific Ocean related to ENSO are the primary driver of slowly propagating signals in the southern Indian Ocean. Wind forcing in the tropical Pacific Ocean drives a surface trapped jet that propagates via the Indonesian Archipelago and excites subsurface variability in meridional velocity in the southern Indian Ocean. In addition, surface heat flux and meridional winds near the west coast of Australia can drive subsurface variability.

Significance Statement
Many previous studies of midlatitude Rossby waves have used satellite altimetry measurements, which reflect variability in the upper few hundred meters of the ocean. Argo float observations have provided in situ hydrographic observations in the upper 2000 m, and these enable us to examine subsurface variability with high reliability. In this study, we used output from an ocean reanalysis, which assimilates in situ observations, and found that the meridional velocity below the surface (∼600-m depth) of the southern Indian Ocean propagates at a phase speed 3 times slower than that of surface variability. These slowly propagating signals can be of climatic importance because of their possible impact on meridional heat transport. We also discuss the driving force of these slowly propagating signals.
Laparoscopic-Assisted Transversus Abdominis Plane Block Is Superior to Port Site Infiltration in Reducing Post-Operative Opioid Use in Laparoscopic Surgery
Authors: Seiler Joclyn , Chong Alexander CM. , Chen Sugong 
Published: April 2022

BackgroundThe ultrasound-guided transversus abdominis plane (TAP) block can be time-consuming, costly, and technically challenging in the bariatric patient population. Laparoscopic-assisted TAP (L-TAP) block was developed and has been shown to be non-inferior to ultrasound-guided blocks. Postoperative pain can be significant, and pain control in the morbidly obese patients can be challenging. This study's aim was to compare L-TAP block to traditional port site infiltration in terms of postoperative opioid requirement for morbidly obese patients after laparoscopic Roux-en-Y gastric bypass (RYGB) surgery.MethodsA retrospective chart review was performed from February 2019 through February 2020. Two study groups: L-TAP block and port site infiltration. Outcomes examined the amount of opioid used at different time segments relative to the operation. All intravenous (IV) and oral opioids used were converted into IV morphine milligram equivalents (MME) for standardization.Results150 patients were included. The patient characteristics were not statistically significant between the two groups. Post-operative opioid use trended lower in the L-TAP block group in all time segments. A significant difference was detected in IV opioid use during post-operative day 0 with the mean MME for the L-TAP block group being 1.1±3.8 and port site infiltration group being 2.8±4.5 (P = .02).ConclusionsThe L-TAP block more effectively reduces postoperative opioid use in comparison to port site infiltration in laparoscopic Roux-en-Y gastric bypass (RYGB) surgery. Based on these findings, as well as the efficiency and cost-effectiveness of L-TAP blocks, its routine use in laparoscopy should be considered.
Upstream Approaches to Addressing Prescription Stimulant Misuse and Diversion: Exploring Perspectives of College Students Who Misuse and Divert
Authors: Roldan Kate Diaz , Garcia Jacquelyn , Valencia Brian , Ayala Jeovanna Amador , Garcia Candelaria , McMullen Jaimie , Bavarian Niloofar 
Published: April 2022

Background We interviewed college students who engage in prescription stimulant misuse and diversion to better understand perspectives on prevention and intervention strategies. Methods Trained student researchers at one southern California university completed 32 face-to-face interviews with students who had a history of misuse and/or diversion. Participants provided insights on programs, policies, and practices that have the potential to deter behavior. Data were analyzed inductively via thematic analysis. Results Students were often misinformed or unaware of existing programs, policies and practices. Additionally, some students felt their behaviors would not be detected, whereas other students felt the fear of being detected would lead them to change their behaviors. Harm reduction and treatment-based approaches to address misuse were also recommended. Conclusions Our findings emphasize the importance of better educating students about, and enforcing, existing policies. In addition, the need to better inform students of existing campus programs is warranted.
A novel anti-lung cancer agent inhibits proliferation and epithelial–mesenchymal transition
Authors: Zhao Wen , Xu Ye , Guo Qingkui , Qian Wenliang , Zhu Chen , Zheng Min 
Published: April 2022

Objective To synthesize a novel chalcone-1,3,4-thiadiazole hybrid and investigate its anticancer effects against NCI-H460 cells. Methods ( E)-3-(4-bromophenyl)-1-(4-hydroxyphenyl)prop-2-en-1-one, 1,3-dibromopropane and 1,3,4-thiadiazole-2-thiol were used as chemical materials to synthesize compound ZW97. The NCI-H460 lung cancer cell line was selected to explore the antitumor effects of compound ZW97 in vitro and in vivo. Results Compound ZW97 selectively inhibited cell proliferation against lung cancer cell lines NCI-H460, HCC-44 and NCI-H3122 with IC50 values of 0.15 μM, 2.06 μM and 1.17 μM, respectively. ZW97 suppressed migration and the epithelial–mesenchymal transition process in NCI-H460 cells in a concentration-dependent manner. Based on the kinase activity results and docking analysis, compound ZW97 is a novel tyrosine-protein kinase Met (c-Met kinase) inhibitor. It also inhibited NCI-H460 cell growth in xenograft models without obvious toxicity to normal tissues. Conclusions Compound ZW97 is a potential c-Met inhibitor that might be a promising agent to treat lung cancer by inhibiting the epithelial–mesenchymal transition process.
Semitendinosus vs Gracilis Grafts With 1- vs 2-Tunnel Techniques for Coracoclavicular Ligament Reconstruction: A Biomechanical Study
Authors: Otto Alexander , Friedman Jamie L. , Szolomayer Lauren K. , Baldino Joshua B. , Obopilwe Elifho , Cote Mark P. , Mazzocca Augustus D. , Arciero Robert A. 
Published: April 2022

Background: Despite the evolution of acromioclavicular joint surgery to a more anatomic coracoclavicular (CC) ligament reconstruction, no definitive guidance regarding the number and position of bone tunnels in the clavicle, as well as the ideal graft choice, is established. Purpose/Hypothesis: The purpose of this study was to biomechanically compare the reconstruction of the CC ligament complex between gracilis- and semitendinosus-tendon grafts in 1- and 2-tunnel techniques. It was hypothesized that the gracilis tendon graft will provide comparable primary stability in both tunnel techniques while utilizing a smaller tunnel diameter. Study Design: Controlled laboratory study. Methods: A total of 24 cadaveric shoulders (13 men, 11 women; 66 ± 7.5 years) were randomly allocated to 4 repair groups: gracilis with 1 tunnel (GT-1), gracilis with 2 tunnels (GT-2), semitendinosus with 1 tunnel (ST-1), and semitendinosus with 2 tunnels (ST-2). First, specimens were tested for native anterior, posterior, and superior translations. Then, specimens were randomly assigned to 1 of the 4 CC reconstruction groups before undergoing the same testing, followed by cyclic loading and load to failure (LTF). Results: The GT-2 reconstruction demonstrated significantly less translation when compared with ST-2 in anterior ( P = .024) and posterior ( P = .048) directions. GT-1 and ST-2 both showed significantly less translation than ST-1 in anterior and superior directions ( P < .001). All reconstructions demonstrated less superior translation compared with native testing, with GT-1 and ST-2 significantly less than ST-1 ( P < .001). There were no significant differences for peak displacement and LTF between groups. Conclusion: Gracilis tendon grafts using a 1- or 2-tunnel technique for CC ligament reconstruction provided comparable translation, displacement, and LTF as corresponding semitendinosus grafts. Therefore, the gracilis tendon should be considered as a biomechanical equivalent graft choice for the reconstruction of the CC ligament complex. Clinical Relevance: In a cadaveric model, the gracilis tendon demonstrated adequate fixation with minimal translation in CC ligament reconstruction while utilizing smaller diameter bone tunnels, which may help minimize the risk of complications such as loss of reduction and fracture.
Racial and Ethnic Differences in Hearing Aid Use Among Medicare Beneficiaries
Authors: Yi Julie S. , Garcia Morales Emmanuel E. , Reed Nicholas S. , Willink Amber , Nieman Carrie L. 
Published: April 2022

Objectives We examined individual-level factors associated with hearing aid use by race and ethnicity in a nationally representative sample of Medicare beneficiaries. Methods We used the Medicare Current Beneficiary Survey (cycles 2016–2018) for 10,301 older adults with hearing loss and hearing aid use as the primary outcome. Covariates included education, income, urban residence, chronic conditions, functional limitations, and Medicaid eligibility. Multivariable logistic regression stratified by race and ethnicity was used to identify factors associated with hearing aid use. Results Factors associated with hearing aid use included higher education among White (OR = 1.35, 95%CI:1.16, 1.58), Black (OR = 1.76, 95%CI:1.02, 3.05), and Hispanic (OR = 1.77, 95%CI:1.17, 2.68) beneficiaries. Urban residence was associated with hearing aid use for Black participants (OR = 3.06, 95%CI:1.17, 8.03) and Medicaid eligibility for Hispanic participants (OR = 1.58, 95%CI:0.97, 2.59), although the confidence interval included the null hypothesis. Discussion ndividual-level factors associated with hearing aid use differed by race and ethnicity among Medicare beneficiaries.
Racial Disparities in the Timing of Alveolar Bone Grafting
Authors: Patmon Darin , Carlson Anna , Girotto John 
Published: April 2022

Standard bone grafting between ages 6 and 12 has become the preferred treatment of choice for alveolar clefts. Given the importance of surgical timing in complete cleft palate repairs, it is important to identify any populations at-risk for delayed alveolar bone grafting. The purpose of this study is to identify whether a racial disparity is present nationally in the timing of alveolar bone grafting. Retrospective analysis Setting: Hospitals participating in the American College of Surgeons National Surgical Quality Improvement Program (ACS-NSQIP). Inclusion criteria involved patients who underwent alveolar bone grafting identified by current procedure terminology (CPT) code 42210 between years 2012 and 2019. Patients were stratified by age at time of operation based on the following parameters: early bone grafting (before 6 years of age), standard bone grafting (between 6 and 12 years of age), and late bone grafting (after 12 years of age). Racial and ethnic differences in the age of patients at the time of alveolar bone grafting. Overall, 20.28% of the cohort received alveolar bone graft after 12 years of age. African American (29.33%) and Hispanic (24.42%) patients received late alveolar bone grafting more frequently than other racial and ethnic groups ( P < .001). Racial and ethnic disparities are present in the frequency at which patients receive late alveolar bone grafting for complete cleft palates. Given the suboptimal surgical results of late compared to standard alveolar bone grafting it is important to further investigate the driving factors of these disparities.
Gram-negative bacteria isolates and their antibiotic-resistance patterns among pediatrics patients in Ethiopia: A systematic review
Authors: Kebede Bekalu , Yihunie Wubetu , Abebe Dehnnet , Addis Tegegne Bantayehu , Belayneh Anteneh 
Published: April 2022

Objective: Antimicrobial resistance is one of the serious threats in the world, including Ethiopia. Even though several studies were conducted to estimate common bacteria and their antibiotic-resistance profile in Ethiopia, it is difficult to estimate the overall resistant patterns due to the lack of a nationwide study. This systematic review aimed to determine the prevalence of gram-negative bacteria isolates and their antibiotic-resistance profile among pediatrics patients in Ethiopia. Methods: A web-based search using PubMed, EMBASE, Science Direct, the Cochrane Database for Systematic Reviews, Scopus, Hinari, Sci-Hub, African Journals Online Library, and free-text web searches using Google Scholar was conducted from August to September 16, 2021. Each of the original articles was searched by Boolean search technique using various keywords and was assessed using the Joanna Briggs Institute Critical Appraisal Checklist. The data were extracted using Microsoft Excel format and exported to STATA 14.0 for statistical analyses. Results: The database search delivered a total of 2,684 studies. After articles were removed by duplications, title, reading the abstract, and assessed for eligibility criteria, 19 articles were included in the systematic review. Of a total of 1372 (16.77%) culture-positive samples, 735 (53.57%) were gram-negative. Escherichia coli was the most frequently isolated bacteria followed by Klebsiella species, 139/1372 (10.13%), and 125/1372(9.11%), respectively. More than 66.67% of isolates were resistant to ampicillin except for Neisseria meningitidis which was 32.35% (11/34). Pseudomonas aeruginosa, Klebsiela species, and Citrobacter species were 100% resistance for cefepime. Haemophilus influenzae was 100% resistant to meropenem. Salmonella species were 93.30%, 78.26%, and 63.64% resistant to tetracycline, chloramphenicol, and cotrimoxazole, respectively. Conclusion: Gram-negative bacteria were identified as the common pathogen causing infection in pediatrics and the level of resistance to commonly prescribed antibiotics was significantly higher in Ethiopia. Culture and susceptibility tests and well-designed infection control programs are important measures.
It’s is Time to Make More Goals So I Can Keep Pushing: Hope, Growth, and Well-Being Among Young Black Women
Authors: Booker Jordan A. , Brakke Karen , Pierre Natasha 
Published: April 2022

Well-being is important for everyone. However, some of our assumptions about well-being and personality have not been well-tested among historically excluded groups. Using a mixed-methods design, we considered young Black women’s hope, growth, and well-being. We hypothesized that endorsements of hope and expressions narrated of growth would be positively associated with well-being. We also tested for the presence of lay themes about well-being in life stories. Fifty-nine Black women from a southeastern US, Historically Black college ( M age = 19.25 years, SD = 1.27) reported on dispositional hope, provided three narratives about life challenges, and completed questionnaires regarding psychological well-being. Consistent with expectations, hope and growth were positively associated with multiple domains of well-being. Further, a content analysis supported existing lay themes of well-being among stories with more growth. Women who expressed growth also incorporated themes of emotional intimacy, competence, moral worth, and regard for their physical and mental health.