bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two different methods of bronchial dissection and coverage in robotic bilobectomy for advanced lung cancer
Authors: Kawaguchi Koji , Ito Atsushi , Kaneda Shinji , Kawaguchi Teruhisa , Shimamoto Akira , Takao Motoshi 
Published: July 2022

AbstractIntroductionDue to its many technical advantages, the scope of robot‐assisted thoracic surgery (RATS) is expanding to include extended pulmonary resection. Among such procedures, right bilobectomy is one with a high risk of inducing development of a bronchial stump fistula.Materials and Surgical TechniqueThe pericardial fat pad case involved a 71‐year‐old man with a 31‐mm adenocarcinoma in the right lung that had progressed to the intermediate bronchus. During lower bilobectomy, to confirm the tumor margin, an L‐shaped stapler was used with stapling only at the oral side, and the bronchus was cut using a scalpel blade grasped with robot forceps. After confirming a negative stump, the pericardial fat was collected at the pedicle and sewn onto the stump. The intercostal muscle (ICM) flap case involved a 61‐year‐old man with a 16‐mm nodule shadow in the lower lobe of his lung and swollen #11i and 7 lymph nodes. Intraoperatively, the #7 lymph node was diagnosed as non‐small‐cell lung cancer by frozen sections, and lower bilobectomy was performed. The bronchus was divided using a stapler with a green cartridge, and the ICM flap was harvested by changing the direction of the camera to a look‐up view and positioning the camera at the 5th intercostal site. His numeric rating score (NRS) at 30 and 90 days post‐surgery was 2 and 0, respectively.DiscussionOur RATS technique was useful for harvesting the ICM flap. More cases should be accumulated to extend the surgical indication for RATS.
Single‐incision laparoscopic partial cecectomy for appendiceal mucocele in a patient with porphyria photosensitivity
Authors: Takami Takuya , Itatani Yoshiro , Shibuya Rintaro , Kiyasu Yoshiyuki , Kasahara Keiko , Nishizaki Daisuke , Okamura Ryosuke , Okada Tomoaki , Nishigori Tatsuto , Hoshino Nobuaki , Hisamori Shigeo , Tsunoda Shigeru , Hida Koya , Kawada Kenji , Obama Kazutaka 
Published: July 2022

AbstractErythropoietic protoporphyria (EPP) is a rare hereditary subtype of cutaneous porphyria characterized by photosensitivity. Increased exposure to light irradiation may precipitate acute liver failure, and surgical light‐induced intestinal burns and perforations are known to occur. We report a case of EPP in a patient who underwent laparoscopic partial cecectomy for appendiceal mucocele. A 55‐year‐old man with EPP was presented for treatment of appendiceal mucocele. A light test using two types of laparoscopes (Companies O and S) was performed preoperatively. Light from the laparoscope manufactured by Company O caused photosensitivity; this effect was not observed with light from the laparoscope manufactured by Company S. Therefore, we performed laparoscopic partial cecectomy through a single umbilical incision using the laparoscope from Company S. Except for the incision site, the patient's skin was completely covered using surgical drapes. No intra‐ or postoperative complications were observed. Histopathological examination of the resected specimen revealed a low‐grade appendiceal mucinous neoplasm.
Laparoscopic resection for recurrent gastrointestinal stromal tumors and paraganglioma in a patient with Carney–Stratakis syndrome: A case report
Authors: Fukada Akio , Takahashi Tsuyoshi , Kurokawa Yukinori , Asaoka Tadafumi , Teranishi Ryugo , Saito Takuro , Yamamoto Kazuyoshi , Yamashita Kotaro , Tanaka Koji , Makino Tomoki , Nakajima Kiyokazu , Umeda Daisuke , Morii Eiichi , Hirota Seiichi , Eguchi Hidetoshi , Doki Yuichiro 
Published: July 2022

AbstractCarney–Stratakis syndrome (CSS) is a familial syndrome characterized by gastrointestinal stromal tumors (GISTs) and paragangliomas, often at multiple sites. A 34‐year‐old woman who had undergone resection of gastric GISTs, liver metastases, and a retroperitoneal paraganglioma in her previous hospital was referred to our hospital due to recurrence after 5 years. She presented with two gastric GISTs, a liver tumor, and a peritoneal tumor. As molecular‐targeted agents are reported to be ineffective against CSS‐related GISTs, we selected surgical resection for the recurrence. We performed laparoscopic local gastrectomy, liver S7 subsegmentectomy, and peritoneal tumor resection. Pathological findings revealed multiple gastric GISTs with liver metastasis and a paraganglioma. The laparoscopic approach could be performed safely, less invasively, and it could be more effective in such cases. This is the first case report of laparoscopic resection for recurrent CSS‐related GISTs and paragangliomas.
Expression and differential posttranscriptional regulation of the elongation factor 1 alpha 1 gene in endometrial caruncle and intercaruncle of Japanese Black cattle at early and mid‐gestation stages
Authors: Komatsu Masanori , Mannen Hideyuki , Taniguchi Masaaki , Oshima Kazunaga 
Published: July 2022

AbstractThe elongation factor 1 alpha 1 (EEF1A1), an isoform of EEF1A, is one of the most abundant cytoplasmic proteins and an important component of the translational machinery. We investigated the relative expression, alternative polyadenylation (APA), and changes in poly(A) tail length of EEF1A1 mRNA in the endometrial caruncle (CAR) and intercaruncle (ICAR) at early and mid‐gestation in Japanese Black cattle. The relative EEF1A1 mRNA expression levels in the CAR were the highest on Gestation day 20 and were significantly decreased at mid‐gestation. The expression levels in the ICAR were significantly higher than those in the CAR, and the gestation stage had no significant impact. Four different EEF1A1 transcripts with distinct 3′ untranslated regions (UTRs) (proximal and distal types) and poly(A) tails (medium and short types) of different lengths were identified. The EEF1A1 mRNAs with distal 3′ UTR and medium‐length poly(A) tails were specific from the CAR of uterus horn at early gestation. RNA‐sequencing data analyses revealed that the HSF1, MZF1, E47, SRF, GATA2, GATA3, GATA6, HNF‐3 beta (FOXA2), CPSF1, and Ataxin‐2 genes might affect the EEF1A1 gene expression or poly(A) length.
Chemical synthesis, biological activities, and molecular simulations of novel sulfonylurea compounds bearing ortho‐alkoxy substitutions
Authors: Shang Ming‐Hao , Zhang Kai , Zhang Jia‐Shuang , Niu Cong‐Wei , Li Yong‐Hong , Song Fu‐Hang , Wang Jian‐Guo 
Published: July 2022

AbstractA series of 51 novel sulfonylurea compounds with ortho‐alkoxy substituent at phenyl ring were chemically synthesized and spectroscopically characterized. The biological activities of the target compounds were evaluated using the enzyme inhibition against acetohydroxyacid synthase (AHAS; EC 2.2.1.6) from fungal or plant source, as well as cell‐based antifungal assay and greenhouse pot herbicidal assay. Among the target compounds, 6e showed desirable antifungal activity against Candida albicans standard isolate sc5314 with minimum inhibition concentration (MIC) of 0.39 mg/L (0.98 μM) after 24 h, and 6a demonstrated promising pre‐emergence herbicidal activity against Echinochloacrus‐galli at 30 g/ha dosage. Representative compounds 6a, 6e, and 6i showed no cell cytotoxicity even at 40 mg/L concentration. Theoretical DFT calculations indicated HOMO maps should be considered to understand the structure–activity relationships. The present study has hence provided useful information for further discovery of novel antifungal agents or selective herbicides.
Synthesis and properties of poly(styrene‐co‐maleic acid) macromolecular reactive dyes
Authors: Wang Fuchun , Ma Wei , Tang Bingtao , Zhang Shufen 
Published: July 2022

AbstractTo obtain a new type of macromolecular reactive dye with high fixation and good light fastness, poly(styrene‐co‐maleic acid) was synthesised by co‐polymerisation of styrene and maleic anhydride, then, through nitration, reduction, diazotisation and coupling reaction, novel macromolecular reactive dyes were prepared with a solubility greater than 60 g/L. The dyes were used to dye cotton fabrics; the results showed their fixation was more than 92%, and both the rub and wash fastness of the dyes were good. The light fastness of the red and blue dyes with the ethoxy group on melamine was grade 4, and for the yellow dye reached grade 5. The poly(styrene‐co‐maleic acid) macromolecular reactive dyes exhibit very good application prospects in practice.
Predictors of time in target glucose range in real‐world users of the MiniMed 780G system
Authors: Castañeda Javier , Mathieu Chantal , Aanstoot Henk‐Jan , Arrieta Arcelia , Da Silva Julien , Shin John , Cohen Ohad 
Published: July 2022

AbstractAimAutomated insulin delivery systems have improved glycaemic control in people with type 1 diabetes mellitus. The analysis investigated predictors of improved sensor glucose time‐in‐range (TIR; 70‐180 mg/dl) based on real‐world use of the MiniMed 780G advanced hybrid closed‐loop (AHCL) system.MethodsData uploaded by MiniMed 780G system users from August 2020‐July 2021 were analysed using univariate and multivariable models to identify baseline, demographic and system use characteristics associated with TIR after AHCL initiation (post‐AHCL). System settings associated with improved TIR post‐AHCL were identified and their impact on time below range (TBR, <70 mg/dl) post‐AHCL was explored.ResultsIn total, 12 870 users were included, of which 2977 had baseline sensor glucose data. Baseline TIR and time in AHCL (defined as the percentage of time the system was in Auto‐mode) were positively associated with TIR post‐AHCL with larger values predicting greater mean TIR post‐AHCL. Characteristics inversely associated with TIR post‐AHCL included the percentage of daily basal insulin dose, daily autocorrection dose, number of daily AHCL exits triggered by the system and number of daily alarms, wherein larger values of these characteristics predicted lower mean TIR post‐AHCL. System settings that predicted the largest mean TIR post‐AHCL were active insulin time of 2 h and glucose target of 100 mg/dl. Active insulin time was not associated with TBR post‐AHCL.ConclusionModifiable factors, including optimized pump settings, can allow users to achieve glycaemic targets with >80% TIR. The findings from this analysis will potentially guide the optimal use of the MiniMed 780G system and facilitate meaningful improvements in safe glycaemic control.
A modelling approach to evaluate land management options for recently burnt catchments
Authors: Basso Marta , Serpa Dalila , Rocha João , Martins Martinho A. S. , Keizer Jacob , Vieira Diana C. S. 
Published: July 2022

AbstractScars left by wildfires are easily visible in the Mediterranean landscape and, like any wound, even those left by fires must be treated to regenerate properly. If not managed, spontaneous vegetation regeneration will eventually cover the affected area, restoring the hydrological cycle. The reforestation of burned stands, involving ploughing the land and building terraces on steep slopes, together with fertilisation of the area, is also common. The Soil and Water Assessment Tool (SWAT) was used to simulate three post‐fire land management options for a small catchment in central Portugal. The choice of this basin relates to the implementation of terraces to create a eucalyptus forest production area 7 months after a fire completely burned the catchment. Two alternative post‐fire scenarios were designed, one simulating the spontaneous recovery of the ecosystem and the second reproducing a catchment‐scale implementation of a highly effective erosion mitigation measure (mulching). Then, the mulch scenario and the actual eucalyptus cycle were compared with the spontaneous recovery scenario to appraise the best management choice. The post‐fire and post‐terracing periods showed a satisfactory agreement between simulated and observed daily parameters. Concerning the management alternatives evaluated, mulching and terracing reduced soil loss and sediment transported in streams compared to spontaneous recovery. However, higher flow volumes and phosphorus exports were predicted under terraced conditions, likely due to increased infiltration capacity and accelerated leaching processes, raising concerns about stream water quality. These findings can be applied to other Mediterranean catchments affected by wildfires and in which post‐fire management actions are planned, making stakeholders aware of the impacts and uncertainties of post‐fire scenarios, thus improving the management of burnt areas.Highlights
Calibration of SWAT model for three different post‐fire management practices.
Satisfactory model performance in simulating water quantity and quality for both periods.
Spontaneous recovery evidenced high soil losses during all the simulated period.
The implementation of terraces and the application of mulch reduced sediment transport.
The gut microbiome and melanoma: A review
Authors: Makaranka Stanislau , Scutt Freya , Frixou Mikaela , Wensley Katherine E. , Sharma Ravi , Greenhowe Jennifer 
Published: July 2022

AbstractDisturbances in the microbial ecosystem have been implemented in chronic inflammation, immune evasion and carcinogenesis, with certain microbes associated with the development of specific cancers. In recent times, the gut microbiome has been recognised as a potential novel player in the pathogenesis and treatment of malignant melanoma. It has been shown that the composition of gut microbiota in early‐stage melanoma changes from in situ to invasive and then to metastatic disease. The gut bacterial and fungal profile has also been found to be significantly different in melanoma patients compared to controls. Multiple studies of immune checkpoint inhibitor (ICI) therapies have shown that the commensal microbiota may have an impact on anti‐tumor immunity and therefore ICI response in cancer patients. When it comes to chemotherapy and radiotherapy treatments, studies demonstrate that gut microbiota are invaluable in the repair of radiation and chemotherapy‐induced damage and therapeutic manipulation of gut microbiota can be an effective strategy to deal with side effects. Studies demonstrate the oncogenic and tumor‐suppressive properties of the gut microbiome, which may play a role in the pathogenesis of melanoma. Despite this, investigations into specific interactions are still in its infancy, but starting to gain momentum as more significant and clinically relevant effects are emerging.
Study on spatio‐temporal variation mechanism of phytic acid contents of wheat grains
Authors: Sun Tianjia , Zhang Yingquan , Wang Shuang , Guo Boli , Yang Qingli , Zhao Haiyan 
Published: July 2022

SummaryThis study aimed to understand the spatio‐temporal variation mechanism of phytic acid in wheat. Firstly, phytic acid contents of wheat from the main producing areas in China were investigated. Then in combination with statistical methods, the effects of the geographical origin, variety, harvest year, their interactions as well as different irrigation frequency on phytic acid contents of wheat were comprehensively analysed based on field experiments. Furthermore, the structure of phytic acid in wheat was analysed by Fourier transform infrared spectroscopy. The phytic acid contents of wheat in China ranged from 6.262 to 9.872 g kg−1. All the factors had significant effects on phytic acid contents of wheat. The characteristic spectra related to phytic acid in wheat included 3348, 1156, 1081, 1019, 927 and 861 cm−1. The variety had the greatest impact on phytic acid contents of wheat. This study could provide reference for breeding wheat with low phytic acid contents.
A superior approach for production of protein isolates from de‐oiled soy meal and its comparison with conventional method
Authors: Yadav Deep Narayan , Tushir Surya , Sethi Swati , Mir Nisar A. , Wadhwa Ritika , Bansal Sangita 
Published: July 2022

SummaryProtein isolates were prepared from de‐oiled soy meal by a superior approach, i.e. biological precipitation using two lactic acid bacterial strains and a conventional acid precipitation method. The isolates were characterised for physico‐chemical, functional, anti‐nutritional and structural properties and compared with isolates prepared using the conventional method. The purity, yield, lightness, whiteness index and degree of hydrolysis were higher (P ≤ 0.05) for biologically precipitated soy protein isolates (BPSPIs). The functional properties of BPSPI samples were also superior. BPSPIs showed 1.85 mg g−1 and 1.44 TIU mL−1, whereas acid‐precipitated soy protein isolates (APSPIs) showed 3.65 mg g−1 and 3.16 TIU mL−1 of phytic acid and trypsin inhibition activity respectively. A sharp bend in the wavelength ranges from 3700 to 3900 cm−1 was observed in BPSPI samples, which depicts structural changes owing to fermentation by lactic acid bacteria. Two characteristic peaks at 2Ɵ = 20° and 2Ɵ = 15° were observed in both the samples; however, intensity and crystallinity were higher in BPSPIs. Hexagonal‐shaped particles of different sizes were displayed by field emission scanning electron microscopy images. The results thus confirm that the soy protein samples prepared through the biological precipitation process had better properties over conventionally prepared soy protein samples, which can be further used as novel ingredients in protein‐based functional products.
Gold nanoparticle‐carbon nanotube nanohybrids with peroxidase‐like activity for the highly‐sensitive immunoassay of kanamycin in milk
Authors: Zeng Kun , Chen Bin , Li Yuxin , Meng Hui , Wu Qinyan , Yang Jian , Liang Hongfang 
Published: July 2022

SummaryOwing to the increasing incidences of antibiotic residues being found in foods of animal‐origin, rapid and reliable screening methods for detecting these residues are urgently required. This study aimed to develop a robust and ultrasensitive colorimetric immunoassay based on nanohybrids for the rapid screening of kanamycin (KAN) residues in milk. The HAuCl4 solution was directly reduced on the multiwalled carbon nanotubes (MWCNTs) using a one‐step synthesis method to obtain the Au/MWCNTs nanohybrids. The Au/MWCNTs exhibited mimetic peroxidase activity and higher affinities for the TMB and H2O2 compared with horseradish peroxidase and other nanohybrids. A direct competitive enzyme‐linked immunosorbent assay (dcELISA) based on Au/MWCNTs was developed and employed for KAN detection in milk samples under optimised conditions. The dcELISA showed high sensitivity toward KAN residues, with a detection limit of 11.2 pg/mL. Moreover, this novel immunoassay displayed high precision and accuracy in milk samples, with a recovery rate ranging from 94.3 to 124.5% and a coefficient variation from 7.8 to 11.3%. Eight real milk samples were found to contain a KAN concentration in the range of ND–0.26 ng/mL. Therefore, this dcELISA based on the Au/MWCNTs nanohybrid has promising potential for applications in food monitoring and quality control.
Characterization of an experimental model to determine streptococcal M protein–induced autoimmune cardiac and neurobehavioral abnormalities
Authors: Rafeek Rukshan AM , Hamlin Adam S , Andronicos Nicholas M , Lawlor Craig S , McMillan David J , Sriprakash Kadaba S , Ketheesan Natkunam 
Published: July 2022

AbstractGroup A streptococcal (GAS) infection is associated with a spectrum of autoimmune diseases including acute rheumatic fever/rheumatic heart disease (ARF/RHD) and neurobehavioral abnormalities. Antibodies against GAS M proteins cross‐react with host tissue proteins in the heart and brain leading to the symptomatology observed in ARF/RHD. As throat carriage of Streptococcus dysgalactiae subspecies equisimilis (SDSE) has been reported to be relatively high in some ARF/RHD endemic regions compared with GAS, and both SDSE and GAS express coiled‐coil surface protein called M protein, we hypothesized that streptococci other than GAS can also associated with ARF/RHD and neurobehavioral abnormalities. Neurobehavioral assessments and electrocardiography were performed on Lewis rats before and after exposure to recombinant GAS and SDSE M proteins. Histological assessments were performed to confirm inflammatory changes in cardiac and neuronal tissues. ELISA and Western blot analysis were performed to determine the cross‐reactivity of antibodies with host connective, cardiac and neuronal tissue proteins. Lewis rats injected with M proteins either from GAS or SDSE developed significant cardiac functional and neurobehavioral abnormalities in comparison to control rats injected with phosphate‐buffered saline. Antibodies against GAS and SDSE M proteins cross‐reacted with cardiac, connective and neuronal proteins. Serum from rats injected with streptococcal antigens showed higher immunoglobulin G binding to the striatum and cortex of the brain. Cardiac and neurobehavioral abnormalities observed in our experimental model were comparable to the cardinal symptoms observed in patients with ARF/RHD. Here for the first time, we demonstrate in an experimental model that M proteins from different streptococcal species could initiate and drive the autoimmune‐mediated cardiac tissue damage and neurobehavioral abnormalities.
Melded ceramic membranes: A novel fabrication method for ultrathin alumina membranes of high performance
Authors: Kirk Chin Ho , Gu Qilin , Ng Tze Chiang Albert , Tan Swee Ching , Ng How Yong , Wang John 
Published: July 2022

AbstractConventional ceramic membranes are usually fabricated on porous ceramic substrates, using a repetitive coating‐sintering process to form a multilayered structure. This results in lengthy fabrication periods and high fabrication costs. In this work, we developed a novel fabrication process, melding, by combining vacuum infiltration and pore tuning techniques to directly construct the ceramic membrane layer within the porous ceramic substrate. By careful selection of powder sizes, the detrimental penetration effect can be modified into a benefit, forming an ultrathin membrane layer with thickness less than 1.45 µm after sintering at 1200°C. Compared favorably with dip‐coated ceramic membranes, the melded ceramic membrane had much smaller nominal pore sizes (0.19 vs 0.31 µm), but much higher water permeance (>60%). The melded ceramic membrane also had the highest rejection (98.5%) toward 125 nm polystyrene latex suspensions (2 ppm), highest sustained oil removal efficiency (>90%) under oil‐in‐water emulsion (200 ppm) filtration, and the least irreversible fouling (<8.6%). The melding process also significantly reduced the raw powders required, leading to an 86% reduction in material costs, as compared to the multilayered ceramic membranes.