bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eco-Physiology of the Rhododedron anthopogon D. Don a dwarf aromatic shrub of the Indian Himalaya
Authors: Trivedi Vijay Laxmi , Maurya Shiva Ji , Sati Pallavi , Jamloki Abhishek , Chandra Sudeep , Nautiyal Mohan Chandra 
Published: December 2022

AbstractRhododendrons are the Himalayan ecological, cultural, aesthetic, and economic entity; some of its varieties are edible and medicinal.Rhododendron anthopogonD. Don is an alpineRhododendronthat grows above tree lines in the Himalaya. This is a dwarfRhododendronwith fragrant leaves that are employed in numerous traditional Himalayan medical systems. This plant's essential oil is pale and contains antibacterial, antioxidant, and insecticidal effects. This manuscript comprises an eco-physiological investigation ofR. anthopogonon an altitudinal and seasonal basis in the Tungnath Himalaya of Uttarakhand, India. We observed that the altitudinal gradient shapes the phyto-sociology ofR. anthopogon, which is one of the dominant species in its habitat. Leaf morphology and phytochemicals (pigments, total soluble sugar, starch and protein, total phenolic content, Malondialdehyde content, Superoxide dismutase and Peroxidase enzyme activities) of theR. anthopogonleaves showed altitudinal and seasonal variations, indicating their importance in thriving in the harsh alpine conditions. The seed germination experiment inR. anthopogonwas also carried and observed that the synergistic effect of cold stratification and GA3reduced the mean germination time while increasing the final germination percentage.
MEMS-based Electronic Textiles Fabricated with Multi-robot Collaboration
Authors: Wei Danming , Challa Sushmita , Islam Mohammad S. , Beharic Jasmin , Harnett Cindy K. , Popa Dan O. 
Published: December 2022

Abstract
This paper presents a methodology to fabricate wafer-scale electronic textiles that is a combination of MEMS-based microclamps and fabric textiles. We describe the capability of deterministic aligning and fastening textile layers with MEMS clamps to create electronic textiles using multi-robot collaboration in the NeXus, a custom additive manufacturing robotic system. To fabricate MEMS clamps, photolithography masks were designed based on the specific locations of intersections in the fabric textiles. The intersection identification was realized via a high-resolution image from the microscope based on a custom image processing algorithm in MATLAB®. To complete the deterministic alignment process of the fabric and clamps, two industrial robotic arms and one custom positioner were employed within our NeXus custom manufacturing system. Also, different robotic process tools were used during the aligning and fastening process of these substrates. Overall, a semi-automated interface was programmed and integrated with the robotic tool change process, visual servoing, target detection, and UV curable adhesive printing functions within the NeXus. The yield of alignment results of the MEMS clamps was assessed, and the completed corresponding fabric intersections were more than 95%.
Factors that Influence the Adoption of Rehabilitation Technologies: A Multi-Disciplinary Qualitative Exploration
Authors: Mitchell Jessie , Shirota Camila , Clanchy Kelly 
Published: December 2022

Abstract
Background
Technological innovation is recognised as having the potential to enhance rehabilitation for people with disability. Yet, resistance to, and abandonment of, rehabilitation technology is prevalent and the successful translation of technology into rehabilitation settings remains limited. Therefore, the aim of this work was to develop an in-depth, multi-stakeholder perspective on what influences the adoption of rehabilitation technologies.
Methods
Semi-structured focus groups were conducted as part of a larger research project aiming to facilitate the co-design of a novel neurorestorative technology. Focus group data were analysed using a five-phase inductive approach to qualitative data analysis.
Results
Focus groups were attended by 43 stakeholders with expertise in one or more of the following fields: people with disability, allied health, human movement science, computer science, design, engineering, ethics, funding, marketing, business, product development, and research development. Six main themes influencing the adoption of technology in rehabilitation were identified: cost beyond the purchase price, benefits to all stakeholders, trust to be earned in technology, ease of technology operation, ability to access technology, and the ‘co’ in co-design. All six themes were found to be interrelated; in particular, the importance of direct stakeholder engagement in the development of rehabilitation technologies (the ‘co’ in co-design) was prevalent in all themes.
Conclusions
A range of complex and interrelated factors influence the adoption of rehabilitation technologies. Importantly, many of the issues that have the potential to negatively impact rehabilitation technology adoption may be addressed during development by utilising the experience and expertise of stakeholders who influence its supply and demand. Our findings state that a wider cohort of stakeholders needs to be actively engaged in the development of rehabilitation technologies to better address the factors that contribute to technology underutilisation and abandonment and facilitate better outcomes for people with disability.
Effect of Benzimidazole Compound on Endoplasmic Reticulum Stress and Unfolded Protein Response Signaling Pathways in Hydrogen Peroxide-Induced BEAS-2B Cells
Authors: Sakar Aysel Eraslan , Kucuk Meral Urhan , Ersan Ronak Haj , Algul Oztekin 
Published: December 2022

Abstract
The main aim of the study was to reveal the effects of endoplasmic reticulum (ER) stress on human bronchial epithelial cells BEAS-2B at gene and protein levels under oxidative stress conditions. The second aim of the study was to investigate whether the benzimidazole compound RHE-231 has a protective effect on ER stress and unfolded protein response (UPR) signaling pathways. To determine subtoxic doses of H2O2 and RHE-231 on cell viability were examined using MTT assay. To determine cellular lipid oxidation of H2O2 was examined using MDA assay. PERK, ATF6, IRE1α, GRP78 mRNA levels and ATF4 and IRE1α protein levels were investigated by quantitative real-time PCR and western blotting, respectively. MDA levels were significantly higher at concentrations of 10 and 20 µM for 24 h compared to the control group. A significant increase in IRE1α and PERK gene expression levels were found at groups of 20 µM H2O2 + 5 µM RHE-231 and 20 µM H2O2 + 10 µM RHE-231 compared to the control group. There were no significant difference observed in ATF6 and GRP78 gene expression levels in any group compared to the control group. According to the results of the western blot analysis, a significant increase were observed at groups of 20 µM H2O2, 20 µM H2O2 + 5 µM RHE-231, and 20 µM H2O2 + 10 µM RHE-231 in the expression of IRE1α and ATF4 compared to the control group. H2O2 mediated oxidative stress in BEAS-2B cells, stimulated ER stress, and activated the UPR pathway. Protein expression was also decreased in some samples where the benzimidazole compound was tested, but this was not in a concentration-dependent manner.
Identification of the Presence of a Novel Variant of CC2D1A Linked to Autosomal Recessive Intellectual Disability 3 in an Iranian Family and Investigating the Structure and Pleiotropic Effects of this Gene
Authors: Rashvand Zahra , Najmabadi Hossein , Kahrizi Kimia , Mozhdehipanah Hossein , Moradi Mohammad , Estaki Zohreh , Taherkhani Khadijeh , Nikzat Nooshin , Najafipour Reza , Omrani Mir Davood 
Published: December 2022

Abstract

Background: Intellectual disability (ID) is a major health problem in our society and it is highly heterogeneous and complex. Numerous genes are involved in the development and normal function of the brain and defects in any of them can result in intellectual disability. Considering that many of these genes have not yet been identified, and those that have been identified have only been found in a small number of patients, therefore, no complete description of the phenotype created by these genes is available. CC2D1A is one of the genes whose loss-of-function mutation leads to a rare form of non-syndromic intellectual disability-3(OMIM*610055), and four pathogenic variants have been reported in this gene so far.
Methods: In the current study, two affected females were included with an initial diagnosis of ID who were from an Iranian family with consanguineous marriage. Whole-exome sequencing was used to identify the probable genetic defects. The Genotypic and phenotypic characteristics of the patients were compared with a mutation in the CC2D1A gene, then the structure of the gene and its reported variants were investigated.
Results: The patients carried a novel homozygous splicing variant (NM_017721, c.1641+1G>A) in exon 14, which is pathogenic according to the ACMG guideline. Loss-of-function mutations in CC2D1A have severe phenotypic consequences such as ID, autism spectrum disorder (ASD), and seizures. However, missense mutations lead to ASD with or without ID, and in some patients, they cause ciliopathy.
Conclusion: This study reports the fifth novel, probably pathogenic variant in the CC2D1A gene. Comparing the clinical and molecular genetic features of the patients with loss-of-function mutation helped to describe the phenotype caused by this gene more precisely. Studying the mutations and structure of the CC2D1A gene revealed that this gene has pleiotropic functions, and the DM14 domain is more critical in causing more severe clinical symptoms such as ID than the C2 domain.
Notes from the field: Descriptive Exploration of childhood tuberculosis frequency and risk factors of zoonotic bovine tuberculosis in Moroto district, Uganda
Authors: Nakayiza Suzan , Walekhwa Abel Wilson , Conlan Andrew , Musewa Angella , Bazeyo William , Kabasa John David 
Published: December 2022

Abstract

Background: Tuberculosis (TB) in children below 15 years is a public health problem in Uganda. Pastoral communities in Moroto district have close association with cattle and have a high burden of TB. This study assessed the frequency of TB among children below 15 years and investigated their anti-TB drug completion rate. In addition, we assessed the knowledge level of community members in pastoral communities on bovine tuberculosis and its risk factors in Moroto district.
Methods: We reviewed registers of TB from five health care centers in Moroto district between September 2020 to May 2021 and data on childhood TB cases was extracted. Community members from pastoral villages were interviewed with a structured questionnaire to assess their knowledge levels on bovine tuberculosis and its risk factors.
Results: A total of 746 tuberculosis cases were recorded in the five TB treatment centers of which, 352 (47 %) cases occurred in children below 15 years. The anti-TB drug completion rate was 222 (63%). Majority of the respondents, 86 % had good knowledge on bovine TB. The common risk factors for bovine TB were sharing water sources with livestock 12 (48 %) and drinking raw milk 21 (84 %).
Conclusion: Childhood TB frequency of 47 % in Moroto district was higher than the national average of 13 %. The Ministry of Health can focus TB health education to childcare takers to reduce transmission and improve drug completion rate. The TB intervention needs to be expanded to develop acceptable practical solutions to overcome the identified risk factors for bovine TB.
David Frank Adjaye Design Thinking
Authors: Putra Hadi Jaya , Sutanty Prisca Bicawasti Budi , Nadia Sarah 
Published: December 2022

Title: David Frank Adjaye’s Design Thinking: The Unfold Cinematically Approach

Design thinking is fundamental to every designer because it becomes a strategy that creatively solves design problems. Adjaye, an architect with various degrees and awards, explicitly stated that the approach used was "The Unfold Cinematically". The excellence of this approach deserves to be expressed to provide insight and inspiration, that designing is not by generalizing the form of design, because every place has its uniqueness. The method used is descriptive qualitative. Data was collected from literature studies, books, articles, and official websites that refer to Adjaye and his works. The results of this study show that this approach reflects his life journey from moving countries so that the character to respect differences in cultural values is formed. "The Unfold Cinematically" then developed into a link between the process consisting of "The Inside" and "The Outside". The conclusion is that the formation of design thinking is a process of a long life journey. Sensitivity to various conditions determines the formation of an architect’s design thinking. The approach of "The Unfold Cinematically" summarizes Adjaye’s focus on the livelihood of social activities and physical forms that are formed from the environment of values held by the community.
Coupled coordination of water resources–economy–ecosystem complex in the Henan section of the Yellow River basin
Authors: Wang Shunsheng , Yang Jinyue , Wang Aili , Yan Yongfang , Liu Tengfei 
Published: December 2022

Abstract
This study investigates the coordination of factors in the three coupled systems of water resources, economy and ecosystem. The study data were economic, social, environmental and water resource indicators of the Henan section of the Yellow River basin from 2000 to 2019. Data were analyzed using a model of coupling coordination, grey relational analysis and a combined sparrow search algorithm–back propagation (SSA-BP) prediction model. Evaluation, analysis and prediction of the water resources–economy–ecosystem complex were undertaken. The results show that: (1) the degree of coupling coordination of the water resources–economy–ecosystem complex in the Henan section of the Yellow River basin showed an increasing trend that reached 0.8105 in 2019, which indicates good coordination; (2) the water resources subsystem had the greatest influence on the overall degree of coupling coordination, followed by the economic and natural environment subsystems; and (3) the SSA-BP model predicted that the degree of coupling coordination would remain good for the next six years and reach 0.8333 in 2025. To ensure the sustainability of expected rapid economic development, Henan Province must increase the utilization efficiency of water resources, strengthen environmental protection, and coordinate development in the region.
A global gridded municipal water withdrawal estimation method using aggregated data and artificial neural network
Authors: Yan Jiabao , Jia Shaofeng 
Published: December 2022

Abstract
Municipal water withdrawal (MWW) information is of great significance for water supply planning, including water supply pipeline networks planning, optimization and management. Currently most MWW data are reported as spatially aggregated over large-area survey regions or even lack of data, which is unable to meet the growing demand for spatially detailed data in many applications. In this paper, six different models are constructed and evaluated in estimating global MWW using aggregated MWW data and gridded raster covariates. Among the models, the artificial neural network-based indirect model (NNM) shows the best accuracy with higher R2 and lower NMAE and NRMSE in different spatial scales. The estimates achieved from the NNM model are consistent with census and survey data, and outperforms the existing global gridded MWW dataset. At last, the NNM model is applied to mapping global gridded MWW for the year 2015 at 0.1 × 0.1° resolution. The proposed method can be applied to a wider aggregated output learning problem and the high-resolution global gridded MWW data can be used in hydrological models and water resources management.
Exploring the potential mechanism of Yixiang capsule immune-enhancing function based on Network Pharmacology, Molecular docking, and Animal function experiment
Authors: Wang Jing , Bai Weirong , Ding Yan , Deng Chenchen , Wang Zhenzhong , Xiao Wei 
Published: December 2022

Background:
The Yixiang capsule with traditional Chinese herbs Cistanches Herba, Hedysarum multijugum Maxim,Fructus Ligustri Lucidi,and Fructus lycii are the main raw materials in a modern herbal medicine preparation containing corresponding bioactive ingredients after extraction and processing. This study aimed to investigate the immune-enhancing function of the Yixiang capsule and explore its potential mechanism.

Methods:
After oral administration of the Yixiang capsule to mice for 30 days, the spleen lymphocyte transformation activity, NK cell activity, DTH, macrophage phagocytosis, HC50 value, amounts of antibody-producing cells, carbon particle clearance rate, spleen, and thymus index were determined to evaluate the regulation effect of the Yixiang capsule on immune function. The potential mechanism underlying the immune-enhancing function of the Yixiang capsule was studied based on network pharmacology and molecular docking.

Results:
The results showed that the Yixiang capsules can obviously increase the HC50 values in all groups, significantly enhance the DTH reaction of mice, the carbon clearance function in medium and high dose groups,and the phagocytic function of macrophages in high dose groups, but had no significant effect on NK cell activity, T lymphocyte proliferation, body weight and immune organ weight of mice. A total of 78 compounds of the Yixiang capsule may affect 123 metabolic pathways and 1022 GO terms by regulating 232 targets, such as the cancer signaling pathway and the signal pathways of response to lipopolysaccharide, and the key targets predicted could stably bind to the core active components of the Yixiang capsule.

Conclusion:
These results suggested that the Yixiang capsule could enhance the immune function of mice and have the characteristics of a multi-component and multi-target synergistic effect on the enhanced immunity function, which provides the basis for the follow-up study.
Preparation of Zinc Oxide Nanoparticles Assisted by Okra Mucilage and
Evaluation of its Biological Activities
Authors: Marzban Abdolrazagh , Shakib Pegah , Mirzaei Seyedeh Zahra , Lashgarian Hamed Esmaeil , Saki Reza , Goudarzi Gholamreza , Alsallameh Sarah , Cheraghipour Kourosh 
Published: December 2022

Background:
In this study, zinc oxide nanoparticles (ZnO-NPs) were biologically synthesized from Abelmoschus esculentus L. (Okra) mucilage fraction (OM).

Methods:
Analytical techniques were employed to study the formation and properties of OM-ZnO NPs, including their morphology, shape, size distribution, and surface charges. Additionally, OM-ZnO NPs were assessed for their antimicrobial, antioxidant, and cytotoxic properties.

Results:
UV-visible spectroscopy confirmed the formation of OM-ZnO NPs, evident by the appearance of an SPR peak at 368.8 nm. The FTIR spectroscopy demonstrated that OM functional groups contribute to the formation and stability of the NPs. Micrographs from TEM and SEM showed that OM-ZnO NPs ranged from 15-40 nm in diameter, whereas hydrodynamic diameter and surface charge values obtained from Zeta and DLS were 72.8 nm and 14.6 mv, respectively. XRD analysis indicated the OM-ZnO NPs were crystalline with a wurtzite structure and a crystallite size of 27.3 nm, while EDX revealed a zinc: oxygen ratio of 67.5:34. Further, the OM-ZnO NPs demonstrated significant antimicrobial activity in response to different types of bacteria. In the antioxidant assay, the OM-ZnO NPs scavenged DPPH with 68.6 % of the efficiency of ascorbic acid (100 %).

Conclusion:
The present study demonstrated the cytotoxic efficacy of MO-ZnO NPs against MCF7 cells with an IC50 of 43.99 µg/ml. Overall, the green synthesis of ZnO NPs by OM was successful for many biological applications, such as antimicrobial, antioxidant, and anticancer. Moreover, OM-ZnO NPs can be applied as a biologically-derived nanotherapeutic agent.
Mass Spectrometry-based Alkaloid Profiling of Cyclea peltata Roots and
Evaluation of In vitro Cytotoxic Activity
Authors: Nekrakaleya Bhagya , Ramaiah Chandrashekar Konambi 
Published: December 2022

Background:
Cyclea peltata (Lam.) Hook. f. and Thoms., an ethnomedicinal herb with
antitoxin and anti-herpes activities, is used to cure smallpox, wounds, diabetic disorders of the
skin, and its use as a cooling agent. In the present study, an attempt has been made to screen different
alkaloids present in Cyclea peltata roots using LC-ESI-MS/MS and to evaluate the in vitro
cytotoxic activity of alkaloids.

Methods:
The detection of alkaloids in C. peltata root extract was carried out by performing
MRM-based assay using liquid chromatography-mass spectrometry. The cytotoxic effect of the
acetone crystalized fraction was performed against human pancreatic cancer using MIA PaCa-2
cells.

Results:
The study revealed the presence of tetrandrine and fangchinoline as the major BBI alkaloids
in the roots of this herb, along with cycleacurine, isochondrodendrine, cycleahomine and
tetrandrine mono-N-2’-oxide. Furthermore, the present study also detected two precursors of BBI
alkaloids such as d-Coclaurine, (-)-N-methylcoclaurine. The study also showed a significant
(p<0.05) cytotoxic effect of the acetone crystalized fraction with an IC50 value of 59.85 μg/ml.

Conclusion:
C. peltata root is the major source of tetrandrine and fangchinoline with potent cytotoxic
effect.
Study of Photo-transformation of Ag Nanoparticles under Green LEDs
Light Source and their Changes Induced by Z-scan Technique
Authors: Ortiz-Jimenez O. , Trejo-Durán M. , Alvarado-Méndez E. , Vázquez A. , Castellanos-Águila J.E. 
Published: December 2022

Background:
The study of metallic nanoparticles is important since they present nonlinear optical properties crucial for modern photonic science and technology. Moreover, their mechanical, chemical, and optical properties are different from those presented with respect to volumetric material. Said properties can be adjusted by controlling the size and shape of the studied nanoparticles, and various methodologies have been developed to obtain nanoparticles by chemical and physical means.

Methods:
Spherical nanoparticles were synthesized by chemically reducing silver nitrate, sodium borohydride, and sodium citrate precursors. Different amounts of silver nitrate were added to the original spherical nanoparticles and then exposed to a green LED light source to convert the spherical nanoparticles to triangular prisms. The changes in the samples were monitored using absorption spectra obtained with a UV-Vis spectrophotometer. The nonlinear refractive index was determined with Z-scan measurements, and a scanning electron microscope was used to observe the silver nanoparticles before and after laser irradiation.

Results:
The absorption spectra show a band of around 418 nm for the original spherical nanoparticles,
which shifted to blue after the irradiation with green LED light. Furthermore, a new
band was obtained, centered around 565 nm, which indicates the presence of triangular prisms.
From SEM images, it was confirmed that the spherical nanoparticles were transformed into triangular
nanoprisms. The non-linear (negative) refractive index depends on the shape and number
of nanoparticles; however, using the Z-scan technique caused photo-melting and photofragmentation
of the triangular prisms, which was corroborated by SEM images.

Conclusion:
These results suggest that the shape and amount of AgNPs can be controlled with excess silver ions and irradiation time. In addition, the Z-scan technique causes photo-melting and photo-fragmentation of AgNPs, and their nonlinear refraction index is negative due to thermal origin.