bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wolbachia infection in field-collected Aedes aegypti in Yunnan Province, southwestern China
Authors: Zhang HengDuan , Gao Jian , Ma Zu , Liu Yuan , Wang Ge , Liu Qing , Du YuTong , Xing Dan , Li ChunXiao , Zhao Teng , Jiang YuTing , Dong YanDe , Guo XiaoXia , Zhao TongYan 
Published: November 2022

BackgroundWolbachia is gram-negative and common intracellular bacteria, which is maternally inherited endosymbionts and could expand their propagation in host populations by means of various manipulations. Recent reports reveal the natural infection of Wolbachia in Aedes Aegypti in Malaysia, India, Philippines, Thailand and the United States. At present, none of Wolbachia natural infection in Ae. aegypti has been reported in China.MethodsA total of 480 Ae. aegypti adult mosquitoes were collected from October and November 2018 based on the results of previous investigations and the distribution of Ae. aegypti in Yunnan. Each individual sample was processed and screened for the presence of Wolbachia by PCR with wsp primers. Phylogenetic trees for the wsp gene was constructed using the neighbour-joining method with 1,000 bootstrap replicates, and the p-distance distribution model of molecular evolution was applied.Results24 individual adult mosquito samples and 10 sample sites were positive for Wolbachia infection. The Wolbachia infection rate (IR) of each population ranged from 0 - 41.7%. The infection rate of group A alone was 0%-10%, the infection rate of group B alone was 0%-7.7%, and the infection rate of co-infection with A and B was 0-33.3%.ConclusionsWolbachia infection in wild Ae. aegypti in China is the first report based on PCR amplification of the Wolbachia wsp gene. The Wolbachia infection is 5%, and the wAlbA and wAlbB strains were found to be prevalent in the natural population of Ae. aegypti in Yunnan Province.
Coagulation- and fibrinolysis-related genes for predicting survival and immunotherapy efficacy in colorectal cancer
Authors: Ma Yanling , Wang Bofang , He Puyi , Qi Wenbo , Xiang Ling , Maswikiti Ewetse Paul , Chen Hao 
Published: November 2022

BackgroundColorectal cancer (CRC) is a common cancer and has a poor prognosis. The coagulation system and fibrinolysis system are closely related to the progression of malignant tumors and is also related to the immunotherapy of malignant tumors. Herein, we tried to predict survival and the immunotherapy effect for patients with CRC using a novel potential prognostic model.MethodsThrough online data of TCGA and GEO, we screened significantly differentially expressed genes (DEGs) to construct a prognostic model, followed by obtaining immune-related genes (IRGs) from the ImmPort database and coagulation- and fibrinolysis-related genes (CFRGs) from the GeneCards database. The predictive power of the model is assessed by Kaplan–Meier survival curves as well as the time-dependent ROC curve. Moreover, univariate and multivariate analyses were conducted for OS using Cox regression models, and the nomogram prognostic model was built. In the end, we further studied the possibility that CXCL8 was associated with immunocyte infiltration or immunotherapy effect and identified it by immunohistochemistry and Western blot.ResultsFive DEGs (CXCL8, MMP12, GDF15, SPP1, and NR3C2) were identified as being prognostic for CRC and were selected to establish the prognostic model. Expression of these genes was confirmed in CRC samples using RT-qPCR. Notably, those selected genes, both CFRGs and IRGs, can accurately predict the OS of CRC patients. Furthermore, CXCL8 is highly correlated with the tumor microenvironment and immunotherapy response in CRC.ConclusionOverall, our established IRGPI can very accurately predict the OS of CRC patients. CXCL8 reflects the immune microenvironment and reveals the correlation with immune checkpoints among CRC patients.
Cortisol awake response imbalance as an indicator of acute central serous chorioretinopathy: Relationship with choriocapillaris and choroidal features
Authors: Scarinci Fabio , Patacchioli Francesca Romana , Costanzo Eliana , Parravano Mariacristina 
Published: November 2022

PurposeThe purpose of the present study was to measure in central serous chorioretinopathy (CSC) the salivary cortisol awake response (CAR) delta percentage (Δ%) variation, a distinct and robust indicator of cortisol rhythm during wakefulness, commonly proposed as a marker of hypothalamic-pituitary adrenal (HPA) axis activity, whose alteration is frequently associated with several adverse health outcomes.MethodsIn the present cross-sectional observational study, salivary CAR Δ% variation was assessed in 17 adult male subjects affected by acute naïve CSC and compared to 17 matched healthy controls. Choroid vasculature metrics were assessed in the study population by measuring the subfoveal choroidal thickness (FCT) and the choroidal vascularity index (CVI) by the imaging technique of enhanced-depth imaging spectral-domain optical coherence tomography (EDI-SD-OCT). Furthermore, flow signal void area features of the choriocapillaris were evaluated in the study population using OCT angiography (OCTA).ResultsBoth the control and CSC groups showed a physiological cortisol increase that occurred during the first 30 min after awaking. However, CSC adult male patients showed remarkably blunted CAR Δ% variation in comparison with controls, which might reflect a CSC-related imbalance of HPA axis activity. Statistically significant correlations were shown by Pearson’s correlation test between salivary CAR Δ% and the selected choroidal and choriocapillaris imaging biomarkers (FCT, CVI, and flow signal void area) in the study population.ConclusionIn conclusion, alterations of the CAR Δ% increase, associated with choroidal-retinal metrics, might provide a window into the physiopathology of acute CSC, suggesting a possible common factor to explain the association between stress and CSC.
Outcomes for critical illness in children with cancer: Analysis of risk factors for adverse outcome and resource utilization from a specialized center in Mexico
Authors: Cardenas-Aguirre Adolfo , Hernandez-Garcia Montserrat , Lira-De-Leon Berenice , Munoz-Brugal Yulissa L. , Wang Huiqi , Villanueva-Diaz Ivonne , Ruiz-Perez Eduardo , Mijares-Tobias Jose M. , Giles-Gonzalez Alex O. , McArthur Jennifer , Escamilla-Aisan Gabriela , Arias Anita , Devidas Meenakshi , Agulnik Asya 
Published: November 2022

IntroductionChildren with cancer have a higher risk of adverse outcomes during critical illness than general pediatric populations. In Low- and middle-income countries, lack of resources can further negatively impact outcomes in critically ill children with cancer.MethodsIn this study, we describe the outcomes of a large cohort of children with cancer including mortality and resource utilization. We performed a retrospective review of all patients admitted to our PICU between December 12th, 2013 and December 31st, 2019. Outcomes were defined as recovery or death and resource utilization was described via use of critical care interventions, Length of stay as well as PICU- and Mechanical Ventilation- free days.ResultsOverall mortality was 6.9% while mortality in the unplanned admissions was 9.1%. This remained lower than expected mortality based on PIM2 scoring. Type of PICU admission, Neurological Deterioration as a cause of PICU admission, and PIM2 were significant as risk factors in univariate analysis, but only PIM2 remained significant in the multivariate analysis.DiscussionOur Study shows that high survival rates are achievable for children with cancer with critical illness in resource-limited settings with provision of high-quality critical care. Organizational and clinical practice facilitating quality improvement and early identification and management of critical illness may attenuate the impact of known risk factors for mortality in this population.
Statistical methods for measuring trends in colorectal cancer incidence in registries: A systematic review
Authors: Alsadhan Norah , Almaiman Alaa , Pujades-Rodriguez Mar , Brennan Cathy , Shuweihdi Farag , Alhurishi Sultana A. , West Robert M. 
Published: November 2022

BackgroundMonitoring cancer trends in a population is essential for tracking the disease’s burden, allocating resources, and informing public health policies. This review describes variations in commonly employed methods to estimate colorectal cancer (CRC) incidence trends.MethodsWe performed a systematic literature search in four databases to identify population-based studies reporting CRC incidence trends, published between January 2010 and May 2020. We extracted and described data on methods to estimate trends and assess model validity, and the software used.ResultsThis review included 145 articles based on studies conducted in five continents. The majority (93%) presented visual summaries of trends combined with absolute, relative, or annual change estimates. Fourteen (10%) articles exclusively calculated the relative change in incidence over a given time interval, presented as the percentage of change in rates. Joinpoint regression analysis was the most commonly used method for assessing incidence trends (n= 65, 45%), providing estimates of the annual percentage change (APC) in rates. Nineteen (13%) studies performed Poisson regression and 18 (12%) linear regression analysis. Age-period-cohort modeling- a type of generalized linear models- was conducted in 18 (12%) studies. Thirty-nine (37%) of the studies modeling incidence trends (n=104, 72%) indicated the method used to evaluate model fitness. The joinpoint program (52%) was the statistical software most commonly used.ConclusionThis review identified variation in the calculation of CRC incidence trends and inadequate reporting of model fit statistics. Our findings highlight the need for increasing clarity and transparency in reporting methods to facilitate interpretation, reproduction, and comparison with findings from previous studies.
Assessing the sustainability capacity of evidence-based programs in community and health settings
Authors: Bacon Caren , Malone Sara , Prewitt Kim , Hackett Rachel , Hastings Molly , Dexter Sarah , Luke Douglas A. 
Published: November 2022

BackgroundWithin many public health settings, there remain large challenges to sustaining evidence-based practices. The Program Sustainability Assessment Tool has been developed and validated to measure sustainability capacity of public health, social service, and educational programs. This paper describes how this tool was utilized between January 2014 and January 2019. We describe characteristics of programs that are associated with increased capacity for sustainability and ultimately describe the utility of the PSAT in sustainability research and practice.MethodsThe PSAT is comprised of 8 subscales, measuring sustainability capacity in eight distinct conceptual domains. Each subscale is made up of five items, all assessed on a 7-point Likert scale. Data were obtained from persons who used the PSAT on the online website (https://sustaintool.org/), from 2014 to 2019. In addition to the PSAT scale, participants were asked about four program-level characteristics. The resulting dataset includes 5,706 individual assessments reporting on 2,892 programs.ResultsThe mean overall PSAT score was 4.73, with the lowest and highest scoring subscales being funding stability and program adaptation, respectively. Internal consistency for each subscale was excellent (average Cronbach's alpha = 0.90, ranging from 0.85 to 0.94). Confirmatory factor analysis highlighted good to excellent fit of the PSAT measurement model (eight distinct conceptual domains) to the observed data, with a comparative fit index of 0.902, root mean square error of approximation equal to 0.054, and standardized root mean square residual of 0.054. Overall sustainability capacity was significantly related to program size (F = 25.6; p &lt; 0.001). Specifically, smaller programs (with staff sizes of ten or below) consistently reported lower program sustainability capacity. Capacity was not associated with program age and did not vary significantly by program level.DiscussionThe PSAT maintained its excellent reliability when tested with a large and diverse sample over time. Initial criterion validity was explored through the assessment of program characteristics, including program type and program size. The data collected reinforces the ability of the PSAT to assess sustainability capacity for a wide variety of public health and social programs.
Antibiotic use, knowledge, and practices of milk vendors in India's informal dairy value chain
Authors: Sharma Garima , Leahy Eithne , Deka Ram Pratim , Shome Bibek R. , Bandyopadhyay Samiran , Dey Tushar K. , Goyal Naresh Kumar , Lundkvist Åke , Grace Delia , Lindahl Johanna F. 
Published: November 2022

BackgroundMilk vendors play an important role in India's dairy value chain; however, their food safety practices are poorly understood. From a milk safety perspective, vendor behavior is significant because it has the potential to affect both consumer and producer behavior. This study describes the types of milk vendors in two Indian states, in an attempt to investigate vendors' hygienic knowledge and practices toward safety and antimicrobial resistance (AMR).MethodsA cross-sectional study was conducted in the states of Assam and Haryana, India. In selected villages, all the milk vendors identified at the time of visit were interviewed. A questionnaire was used to assess the knowledge and practices on antibiotics, milk safety and hygiene. The milk samples were tested for presence of antibiotic resistant bacteria using antibiotic susceptibility testing.ResultsIn total, 244 milk vendors were interviewed during the survey. Out of these, 146 (59.8%) of the vendors traded raw milk, while 40.2% traded pasteurized milk. Vendors were categorized depending on whom they supplied milk to. Five categories were identified: (a) those who sold at grocery shops; (b) those who sold on roadside (roadside vendors); (c) those who sold from door to door; (d) those who sold to sweet makers/tea stalls, and (e) those who sold from own home/other entity. The level of training among vendors on milk hygiene was non-existent and the knowledge related to antibiotics was low. Most of them [210/244 (86.07%)] agreed that boiled milk is always safer than raw milk but almost half [119 (48.77%)] of them admitted that sometimes they drink milk without boiling it. Most vendors believed that they could identify whether milk is safe or not for consumption just by its appearance and smell. Out of 124 milk samples collected from surveyed milk vendors and tested for the presence of antibiotic-resistant bacteria, 80 (64.52%) were tested positive.ConclusionThis study highlights the low levels of knowledge regarding food safety among milk vendors. It shows the predominance of informal milk vendors in the surveyed states and prevalence of AMR bacteria in milk traded by them. Training may be a beneficial strategy for addressing the issue.
Socioeconomic Status, the Countries’ Socioeconomic Development and Mental Health: Observational Evidence for Persons with Spinal Cord Injury from 22 Countries
Authors: Fekete Christine , Tough Hannah , Leiulfsrud Annelie Schedin , Postma Karin , Bökel Andrea , Tederko Piotr , Reinhardt Jan D. 
Published: November 2022

Objectives: Evidence on social inequalities in mental health of persons with physical impairments is limited. We therefore investigate associations of individual-level socioeconomic status (SES) and the country-level socioeconomic development (SED) with mental health in persons with spinal cord injury (SCI).Methods: We analyzed data from 12,588 participants of the International SCI Community Survey from 22 countries. To investigate individual-level inequalities, SES indicators (education, income, financial hardship, subjective status) were regressed on the SF-36 mental health index (MHI-5), stratified by countries. Country-level inequalities were analyzed with empirical Bayes estimates of random intercepts derived from linear mixed-models adjusting for individual-level SES.Results: Financial hardship and subjective status consistently predicted individual-level mental health inequalities. Country-level SED was inconsistently related to mental health when adjusting for individual-level SES. It however appeared that higher SED was associated with better mental health within higher-resourced countries.Conclusion: Reducing impoverishment and marginalization may present valuable strategies to reduce mental health inequalities in SCI populations. Investigations of country-level determinants of mental health in persons with SCI should consider influences beyond country-level SED, such as cultural factors.
Symbiotic System Establishment between Piriformospora indica and Glycine max and Its Effects on the Antioxidant Activity and Ion-Transporter-Related Gene Expression in Soybean under Salt Stress
Authors: Zhang Depeng , Wang Xinsheng , Zhang Zhenyue , Li Chunxin , Xing Yimei , Luo Yaqin , Li Donghuan , Ma Zhiyun , Cai Hua 
Published: November 2022

The utilization of symbiosis with beneficial microorganisms has considerable potential for increasing growth and resistance under abiotic stress. The endophytic root fungus Piriformospora indica has been shown to improve plant growth under salt and drought stress in diverse plant species, while there have been few reports of the interaction of P. indica with soybean under salt stress. In this study, the symbiotic system of P. indica and soybean (Glycine max L.) was established, and the effect of P. indica on soybean growth and salt tolerance was investigated. The colonized and non-colonized soybeans were subjected to salt stress (200 mmol/L NaCl), and the impairments in chlorophyll and increasing relative conductivity that can be caused by salt stress were alleviated in the P. indica-colonized plants. The accumulation of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion (O2−) were lower than that in non-colonized plants under salt treatment, whereas the activities of antioxidant enzymes were significantly increased by P. indica colonization, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione reductase (GR). Importantly, without salt treatment, the Na+ concentration was lower, and the K+ concentration was higher in the roots compared with non-colonized plants. Differential expressions of ion transporter genes were found in soybean roots after P. indica colonization. The P. indica colonization positively regulated the transcription level of PM H+-ATPase, SOS1, and SOS2. The study shows that P. indica enhances the growth and salt tolerance of soybean, providing a strategy for the agricultural production of soybean plants in saline-alkali soils.
Rickettsial Agents Associated with Ectoparasites in Attica, Greece
Authors: Liodaki Maria , Angelakis Emmanouil , Spanakos Gregory , Papadogiannaki Ioanna , Samarkos Michael , Daikos George L. , Christopoulou Barbara , Piperaki Evangelia-Theophano 
Published: November 2022

The bacteria of the families Rickettsiaceae and Anaplasmataceae, harbored by arthropod vectors, may cause disease in animals and humans. The aim of this study was to screen ectoparasites collected from cats and dogs in Attica, Greece for the bacteria of the Rickettsiales group, by molecular methods. The ectoparasites examined were Ctenocephalides felis fleas and Rhipicephalus sanguineus s.l., Rhipicephalus sp., and Ixodes sp. ticks. Rickettsia felis was detected in 4.8% of C. felis fleas, and Rickettsia conorii was detected in 7.3% of R. sanguineus s.l. ticks. Ehrlichia canis was found in one R. sanguineus s.l. tick, and Wolbachia pipientis was detected in the majority of fleas. Another endosymbiont, Cancidatus Midichloria mitochondrii (Cancidatus Midichloriaceae), was detected in one Ixodes sp.. This is the first report of R. conorii and E. canis in R. sanguineus s.l. ticks in this study area. Given the fact that Greece is considered endemic for spotted fever group rickettsioses, further investigation of these rickettsial pathogens’ distribution in their vectors and hosts could enhance our knowledge of their epidemiology, in order to assess their potential implications for public health in this metropolitan area.
Strategi Dinas Ketenagakerjaan dalam Meningkatkan Kesempatan Kerja bagi Penyandang Disabilitas di Kabupaten Sidoarjo
Authors: Hariyanti Kiki , Sukmana Hendra 
Published: November 2022

This study aims to determine the Strategy of the Manpower Office in Increasing Employment Opportunities for Persons with Disabilities. The sampling technique in this study is snowball sampling technique. The approach in this study uses a qualitative approach with a descriptive method. Important insights were obtained through interviews and observations related to the strategy of the Sidoarjo Regency Manpower Office in developing the employment potential of persons with disabilities. Meanwhile, optional information is collected through reports such as Sidoarjo Disnaker Records, Related Domain Records, Principles and Regulations, Widely Communicated Data, Other Information and Data on the employment potential of persons with disabilities. The primary data was tried through the Triangulation Technique. The Triangulation technique used in this examination is to compare observational information and interview information and compare with the content of related documents. From the results of this study, it is shown by the results of interviews from various parties, that the provisions of the Sidoarjo Regency regulations on Persons with Disabilities are still not optimally determined. There are contributing factors that cause the employment opportunities of people with disabilities to be narrow, one of which is the low level of education.
Notas sobre a possibilidade de reflexos agostinianos na conceituação de teofania em João Escoto Erígena
Authors: Pignatari Roberto Carlos 
Published: November 2022

Embora explicitada em vários momentos ao longo da obra de Erígena, a presença do pensamento agostiniano em suas especulações centrais nem sempre é manifesta e segura. A partir da ontologia relacional trabalhada por Agostinho no De Trinitate IX-XII, com base em sua redefinição da categoria aristotélica de relação no livro V desta obra, pretendemos apontar para seus possíveis reflexos na herança patrística operada por Erígena no Periphyseon I, iniciando pela polaridade central ser – não-ser, onde toma corpo a noção de ser como expressão perceptível e dizível, e na qual os polos são ditos e inteligidos unicamente de modo relacional recíproco; caminhando pela conceituação de natureza como expressão das coisas que são e, portanto, existem e são cognoscíveis (ao contrário das essências, incognoscíveis); e culminando na cristalização do conceito epicentral de seu pensamento: teofania, pelo qual se vislumbra a realidade natural como expressão divina, em que o Criador se expressa criando (e se manifestando, portanto dando-se a ser conhecido na) a natureza.
Palavras-chave: Ser, Expressão, Relação, Teofania, João Escoto Erígena.