bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing Thailand’s 1-3-7 surveillance strategy in accelerating malaria elimination
Authors: Sudathip Prayuth , Naowarat Sathapana , Kitchakarn Suravadee , Gopinath Deyer , Bisanzio Donal , Pinyajeerapat Niparueradee , Sintasath David , Shah Jui A. 
Published: February 2022

Abstract
Background: Thailand’s strong malaria elimination program relies on effective implementation of its 1-3-7 surveillance strategy, which was endorsed and implemented nationwide in 2016. For each confirmed malaria patient, the Ministry of Public Health’s Division of Vector Borne Diseases (DVBD) ensures completion of case notification within 1 day, case investigation within 3 days, and foci investigation within 7 days. To date, there has not been a comprehensive assessment of the performance and achievements of the 1-3-7 surveillance strategy although such results could help Thailand’s future malaria elimination strategic planning. Methods: This study examined adherence to the 1-3-7 protocols, tracked progress against set targets, and examined geographic variations in implementation of the 1-3-7 strategy in the program’s initial five years. An auto-regressive integrated moving average (ARIMA) time series analysis with seasonal decomposition assessed the plausible implementation effect of the 1-3-7 strategy on malaria incidence in the program’s initial five years. The quantitative analysis included all confirmed malaria cases from public health and non-governmental community facilities from October 2014 to September 2021 (fiscal year [FY] 2015 to FY 2021) (n = 77,405). The spatial analysis included active foci with known geocoordinates that reported more than five cases from FY 2018 to FY 2021.Results: From FY 2017 to FY 2021, on-time case notification improved from 24.4% to 89.3%, case investigations from 58.0% to 96.5%, and foci investigations from 37.9% to 87.2%. Adherence to timeliness protocols did not show statistically significant variation by area risk classification. However, adherence to 1-3-7 protocols showed a marked spatial heterogeneity among active foci, and the ARIMA model showed a statistically significant acceleration in the reduction of malaria incidence. The 1-3-7 strategy national indicators and targets in Thailand have shown progressive success, and most targets were achieved for FY 2021. Conclusion: The results of Thailand’s 1-3-7 surveillance strategy are associated with a decreased incidence in the period following the adoption of the strategy although there is notable geographic variation. The DVBD will continue to implement and adapt the 1-3-7 strategy to accelerate progress toward malaria elimination. This assessment may be useful for domestic strategic planning and to other countries considering more intensive case and foci investigation and response strategies.
Evaluating Croton macrostachyus: honey, nectar, and pollen antimicrobial activities against Escherichia coli, Shigella boydii, Staphylococcus aureus, and Bacillus subtilis
Authors: Roba Kasim , Bedewi Zufan 
Published: February 2022

Abstract
Background: Ethiopia is one of the plant species-rich countries in the world and the center of origin of many medicinal plants. Studying antimicrobial activities is vital to investigate plants resources for medicinal values and the study was conducted to identify and evaluate Croton macrostachyus honey’s antimicrobial sources from its nectar and pollen against mentioned bacteria. Methods: Completely Randomised Design was used for the laboratory. After adjusting turbidity, consistent growth of bacterial culture was made using sterilized cotton. The extract of C. macrostachyus’ pollen 3.6 gm. was added to 12 ml of distilled water to prepare stock solutions as 3.6:12 = 0.3 x B = 3 x a ppm stock solution and antimicrobial activities of pollen, nectar, and honey were tested against mentioned bacteria above. Data were inserted into Microsoft excel 2010 and imported to R software version 3.44. Multilevel analysis was used to see the interaction between bacteria species and each concentration of honey, nectar, and pollen of Croton and Anova was used to see the significance of these concentrations on bacteria species. A p-value of <0.05 was considered statistically significant.Results: Results indicated that bacteria were more inhibited at 72 hours than 48 and 24 hours and the result identified honey’s antimicrobial source was due to pollen composition found in honey that only pollen had antimicrobial activities while nectar had no antimicrobial activities against both Gram-negative and Gram-positive bacteria mentioned above. Time has significant effects on tested bacteria (p = 0.000) and treatments have significant effects on tested organisms (p = 0.000). Honey inhibited the growth of more Gram-negative bacteria: Escherichia coli and Shigella boydii. Bacillus was mostly inhibited by crotons’ pollen than others.Conclusions: Water extract of Crotons ‘pollen had antibacterial activities against all tested bacterial strains, but inhibited more Gram-positive bacteria; while Crotons’honey inhibited more Gram-negative bacteria than Gram-positive bacteria. Negative controls (sterilized water) and nectar did not show an inhibitory effect on tested bacteria, while positive control (Chloramphenicol) had antimicrobial activities. Further isolation and characterization of bioactive compounds from Croton macrostachyus pollen are useful to develop a novel botanical formulation for further applications.
Aptamer-Functionalized Quercetin Thermosensitive Liposomes for Targeting Drug Delivery and Antitumor Therapy
Authors: Li Jian , Gao Yanting , Liu Shihe , Cai Jiahui , Zhang Qing , Li Kun , Liu Zhiwei , Shi Ming , Wang Jidong , Cui Hongxia 
Published: February 2022

Abstract
Chemo-thermotherapy, as a promising cancer combination therapy strategy, has attracted widespread attention. In this study, a novel aptamer functionalized thermosensitive liposomes encapsulating hydrophobic drug quercetin were fabricated as an efficient drug delivery system. This aptamer-functionalized quercetin thermosensitive liposomes (AQTSL) combined the merits of high-loading yield, sustained drug release, long-term circulation in the body of PEGylated liposomes, passive targeting of 100-200 nm nanoparticles, active targeting and improved internalization effects offered by AS1411 aptamer, and quercetin release controlled by temperature-response and thermotherapy. In addition, AQTSL tail vein injection combined with 42℃ administration inhibited the tumor growth significantly compared with the saline administration (p<0.01), and the inhibition rate reached 75%. Furthermore, AQTSL combined with 42℃ administration also slowed down the tumor growth significantly compared with QTSL combined with 42℃ administration (p<0.05), which further confirmed that AS1411 decoration increased the active targeting and internalization effects of the drug delivery system, and AS1411 aptamer itself might also contribute to the tumor inhibition process. These data indicated that AQTSL is a potential carrier candidate for different hydrophobic drug tumor targeting delivery and this kind of targeted drug delivery system combined with temperature response drug release mode is expected to achieve an ideal tumor therapy effect.
Transcriptomic and Functional Approaches Unveil the Role of tmRNA in Zinc Acetate Mediated Levofloxacin Sensitivity in Helicobacter Pylori
Authors: Tao Hongjin , Meng Fansen , Zhou Yu , Fan Jiao , Liu Jing , Han Yingjie , Sun Benjamin B. , Wang Gangshi 
Published: February 2022

Abstract
Background: Rapid increase in resistance of Helicobacter pylori (H. pylori) has hindered antibiotics-based eradication efforts worldwide and raises the need for additional approaches. Here, we investigate the role of zinc-based compounds in inhibiting H. pylori growth and modulating antibiotic sensitivities, interrogate their downstream transcriptomic changes and highlight the potential mechanism driving the observed effects.Results: We showed that zinc acetate inhibited H. pylori growth and increased H. pylori sensitivity to levofloxacin. Transcriptomic profiling showed distinct gene expression patterns between zinc acetate treated groups vs controls. In particular, we independently replicated the association between zinc acetate treatment and increased ssrA expression. Knockdown of ssrA restored levofloxacin resistance to levels of the control group.Conclusion: In this study, we first demonstrated the role of zinc acetate in H. pylori growth and antibiotic sensitivities. Additionally, we explored the transcriptomic perturbations of zinc acetate followed by functional knockdown follow-up of differentially expressed ssrA, highlighting the role of tmRNA and trans-translation in H. pylori levofloxacin resistance. Our results provide alternative and complementary strategies for H. pylori treatment and shed light on the underlying mechanisms driving these effects.
Comparative Proteomic Analysis of Taenia solium Cysticercus and Adult Stages
Authors: Li Lizhu , Zhou Biying , Hi Wei , Fan Xianmin , Liu Meichen , Luo Bo , Yang Fengjiao , Jiang Nan , Wang Lingjun 
Published: February 2022

Abstract
B ack ground : Cysticercosis is one of 20 tropical diseases classified as neglected by the World Health Organization (WHO). As the pathogenesis of cysticercosis is not yet clear, there is no vaccine that can completely cure the disease. This study attempts to identify the proteins of adult and larvae of Taenia solium by mass spectrometry, predict the function of differential proteins so as to further study the Pathogenic mechanism of larvae to host. This provides clues for the cysticercosis prevention and treatment.
Methods: In our study, we separated proteins of adult and larvae of Taenia solium by High Performance Liquid Chromatography(HPLC) fractionation, and protein samples were also digested in liquid and identified by liquid chromatography tandem mass spectrometry(LC-MS/MS); the differentially expressed proteins were then processed by a bioinformatics and parallel reaction monitoring (PRM) analysis .
Results: Our results showed that the 3658 proteins identified, 2481 contained quantitative information, according to the relative quantitative analysis of the protein expression level by label-free quantification, differentially expressed proteins between the two treatments were identified applying a P value < 0.05 and a twofold change threshold, of which 293 proteins were up-regulated whereas 265 proteins were down-regulated. Through the bioinformatics analysis, we found some proteins that were associated with the immune effector process in addition to diagnostic antigens and therapeutic targets.
Conclusion: We conclude that the results facilitate a better understanding of the complexity of the cestode life cycle, mechanism to counter the host immune system, and host–parasite interactions in both developmental stages. Evading the host immune response may be key for the establishment of latent and persistent infections by cysticercus. Therefore, understanding the mechanisms through which the parasites manipulate the host immune response for their survival is important to our understanding of the intricate parasite-host interaction. Further studies are required to identify what function those proteins are, it may contribute to the development of novel strategies and vaccines against cysticercosis.
FACTORS THAT INFLUENCE ACCOUNTING STUDENT'S INTEREST IN A CAREER AS PUBLIC ACCOUNTANT
Authors: SEKARINI GALUH DWI , Khoiriawati Novi 
Published: February 2022

Abstrak: Penelitian ini dilatar belakangi oleh sedikitnya tenaga kerja akuntan profesional di Indonesia sehingga mengakibatkan persaingan yang ketat dengan negara lain. Dan kurangnya minat sarjana akuntansi mengambil profesi sebagai akuntan menjadi salah satu penyebabnya. Maka dari itu, dengan diketahuinya pilihan karir yang diminati mahasiswa, maka dapat diketahui mengapa seseorang memilih karir tersebut. Minat dan rencana karir yang jelas akan sangat berguna dalam menentukan karir dimasa mendatang.
Rumusan masalah dalam penelitian ini meliputi : 1) Apakah penghargaan finansial berpengaruh terhadap minat mahasiswa akuntansi dalam berkarir sebagai akuntan publik? 2) Apakah pengakuan profesional berpengaruh terhadap minat mahasiswa akuntansi dalam berkarir sebagai akuntan publik? 3) Apakah pertimbangan pasar kerja berpengaruh terhadap minat mahasiswa akuntansi dalam berkarir sebagai akuntan publik? 4) Apakah risiko profesi akuntan berpengaruh terhadap minat mahasiswa akuntansi dalam berkarir sebagai akuntan publik? 5) Apakah penghargaan finansial, pengakuan profesional, pertimbangan pasar kerja, dan risiko profesi akuntan berpengaruh terhadap minat mahasiswa akuntansi dalam berkarir sebagai akuntan publik?
Dalam penelitian ini pendekatan yang digunakan adalalah pendekatan kuantitatif, jenis penelitian deskriptif. Sumber data yang digunakan adalah data primer dengan jumlah sampel sebanyak 165 responden. Teknik pengumpulan data yang digunakan adalah kuesioner. Pengolahan data menggunakan SPSS 26 untuk uji validitas, reliabilitas, uji normalitas, uji multikolinieritas, uji heteroskedastisitas, uji autokorelasi, serta uji regresi linier berganda (uji t dan uji F).
Hasil penelitian ini menyimpulkan bahwa secara parsial variabel penghargaan finansial, pengakuan profesional, pertimbangan pasar kerja, dan risiko profesi akuntan berpengaruh positif dan signifikan terhadap minat mahasiswa akuntansi dalam berkarir sebagai akuntan publik pada mahasiswa akuntansi syariah IAIN Tulungagung.
THE EFFECT OF TAX RATE, TAX AWARENESS AND PENALITIES ON TAX PAYER COMPLIANCE AT SMEs IN REJOTANGAN TULUNGAGUNG
Authors: Rahayu Novita Eksanti 
Published: February 2022

Abstract The purpose of this research is to examine the effect of tax rates on taxpayer compliance of SMEs in Rejotangan Tulungagung district. To test the effect of sensitization on taxpayer compliance with SMEs in Rejotangani Tulungagung District, to examine the effect of tax penalties on SMEs taxpayer compliance in Rejotangani Tulungagung District. To examine the effect of tax rates, tax awareness and penalties on taxpayer compliance by SMEs in Rejotangan Tulungagung District. The research approach used is a quantitative approach, of the associative research type. The population used in this study consisted of all SMEs in Rejotangan district, with a total of 85 SMEs selected using the random sampling technique. Data analysis used the multiple linear regression method. The results of this study, namely: (1) There is a significant effect of tax rates on taxpayer compliance of SMEs actors in Rejotangan Tulungagung district. The amount of the tax rate which is the stipulation also makes the reason why the taxpayer pays the tax. (2) There is a significant effect of sensitization on tax compliance of SMEs in Rejotangan Tulungagung district. Taxpayers make tax payments due to self-awareness or encouragement from other parties. (3) Tax penalties have a significant effect on taxpayer compliance of SMEs actors in Rejotangan Tulungagung district. The application of tax penalties that create a deterrent effect, so that subsequent payments are better directed. (4) There is a significant effect of tax rates, awareness and tax penalties on taxpayer compliance of SMEs actors in Rejotangan Tulungagung district. Therefore, it is important for the parties involved to consider various factors that influence taxpayer compliance.
Keywords: SMEs, tax rate, tax awareness, penalties for taxpayer compliance
Spatiotemporal variations in the elasticity of runoff to climate change and catchment characteristics with multi-timescales across the contiguous United States
Authors: Gong Xinglong , Xu Aiqi , Du Shuping , Zhou Yuehan 
Published: February 2022

Abstract
There is a lack of understanding of the impact of timescales on runoff elasticity. In this study, gauged river flow and meteorological data time-series from 1916 to 2015 and 1941 to 2015 across the contiguous United States (CONUS) for 188 catchments were collected. We studied spatiotemporal variations of climate and catchment elasticity at multi-timescales (11, 20, 40, and 60 a) across the CONUS, and the relationships between the elasticity of runoff and climatic factors were also analyzed. Median descriptive statistics and Budyko-coupled climate elasticity methods were used to calculate runoff elasticity in each timescale. The original Mann–Kendall trend test was used to test the temporal trend significance in four timescales, respectively. The results show that (1) the trend and values of runoff elasticity may not be affected by timescales. (2) Catchment characteristics in some western regions may be the second-most sensitive factor for runoff following the maximum temperature. The roles of potential evaporation, maximum temperature, and catchment characteristics on runoff gradually become important from east to west. (3) Runoff elasticity is independent of precipitation, and wet regions tend to have lower elasticity of runoff. There are strong relationships between elasticity and most factors, except for precipitation, which can illustrate well its spatial pattern.
Asistan hekimlerde korona virüs salgını nedeniyle yaşanılan zorluklar, kaygı düzeyi ve tükenmişlik sendromunun incelenmesi
Authors: TÜRKİLİ Seda , ASLAN Eda , TOT Şenel , ERDOĞAN Semra , GÜLERYÜZ Gamze , ERDOĞAN AKTÜRK Beyza , ÖZASLAN Emre 
Published: February 2022

Amaç: Yeni tip koronavirüs (COVID-19) pandemisi dünya çapında sağlık çalışanlarını gerek fiziksel gerekse ruhsal açıdan etkileyecek birçok zorluğu beraberinde getirmiştir. Bu çalışmada amaç, COVID-19 pandemisi sürecinde görev alan asistan hekimlerin karşılaştıkları zorlukların, kaygı yaratan durumların belirlenmesi ve tükenmişlik sendromu ve ilişkili faktörlerin değerlendirilmesi ve risk faktörleri için bir çerçeve sunulmasıdır.Yöntemler: Çalışmamız internet üzerinden uygulanan bir anket çalışması olup, hastanemizin asistan hekimlerine sosyal platformlar üzerinden uygulanmıştır. 82 kadın, 73 erkek toplam 155 katılımcı anketi tamamlamıştır. Ankette sosyodemografik veriler ve COVID-19 pandemisi deneyimleri soru formu, Hasta Sağlık Anketi-4 ve Maslach Tükenmişlik Ölçeği kullanılmıştır.Bulgular: Çalışmamızda katılımcıların % 61,3’ünün (n=95) anksiyete, % 58,1’inin (n=90) depresyon yaşadığı saptanmıştır. Tükenmişlik alt ölçeklerinde ortalama puanlar “duygusal tükenme” için 21,4 (±8,4), “duyarsızlaşma” için 8,65 (±5,10) ve “kişisel başarı” için 12,6 (±5,8) olarak bulunmuştur. COVID-19 hastaları ile artmış temas sıklığı, algılanan bulaş riski, idareciler tarafından algılanan destek eksikliği, sosyal destek eksikliği, damgalanma ve aşırı sosyal medya kullanımı ile depresyon artışı, anksiyete ve tükenmişlik seviyeleri arasında ilişki olduğu saptanmıştır.Sonuç: Pandemi sürecinde sağlık hizmetlerinin etkin ve kaliteli bir şekilde sürdürülmesi, bu süreçte kilit rol oynayan asistan hekimlerin gerek fiziksel gerekse ruhsal açıdan iyi oluş hali ile doğrudan ilişkilidir. Bu süreçte görev alan sağlık çalışanlarının pandemi hakkında doğru bilgiye zamanında erişimlerinin sağlanması, kişisel koruyucu ekipmanlara yeterli erişim, çalışılan kurum idarecilerinin yanı sıra aile ve sosyal çevrenin desteği ile dışlanma damgalanma deneyimlerinin en aza indirgenmesi ve sosyal medya, TV gibi mecralar kanalıyla yoğun bir biçimde pandemi haberlerine ve yanlış bilgilere maruz kalmanın engellenmesi ruhsal açıdan koruyucu olacaktır. Yine mesai düzenlemelerinin enfekte hastalar ile temas sıklığını en alt düzeyde tutacak şekilde planlanması algılanan tehdit ve buna bağlı ruhsal etkilenmeleri azaltmada etkili olacaktır.
Aplicación de herramientas digitales en la enseñanza-aprendizaje de lenguas-culturas
Authors: Jiménez Llamas Emma 
Published: February 2022

En este artículo expondremos cómo hemos implementado tres herramientas tecnológicas en un proyecto de enseñanza-aprendizaje del Español, LE: la revista electrónica Flores de Nieve, su blog y una wiki. Este proyecto, que se inició en 1998 en el campus de la Universidad Nacional Autónoma de México en Canadá, ha permitido que estudiantes y profesores de muy diversos países interactúen en español en una modalidad mixta. El proyecto consistió en una primera etapa en elaborar una revista y publicarla en línea como medio para aprender la lengua-cultura meta.
Expondremos a continuación los objetivos que han guiado a estudiantes y profesores en el proyecto, y cómo incorporamos otro género digital, un blog, para dar seguimiento al diálogo entre autores y lectores.
Describiremos el procedimiento que lleva de las tareas pedagógicas de expresión escrita en clase a la revisión colaborativa de los textos en una wiki, de dónde seleccionan los estudiantes lo que desean publicar en la revista, y la manera en que se establece la interacción en el blog con sus lectores.
Concluiremos con una reflexión sobre lo que ha aportado la aplicación de estas tres herramientas a la enseñanza-aprendizaje de la lengua-cultura meta y su proyección en el futuro.
Dale valor a tu español
Authors: Alegría Fernández María Sara , Lugo Martínez Ileana Betzabeth 
Published: February 2022

SIELE es el Servicio Internacional de Evaluación de la Lengua Española que certifica el grado de dominio del español a través de medios electrónicos dirigido a estudiantes y profesionales de los cinco continentes.
La Universidad Nacional Autónoma de México, el Instituto Cervantes, la Universidad de Salamanca y la Universidad de Buenos Aires, son las instituciones titulares del SIELE, lo que garantiza estándares de calidad y buenas prácticas en la creación de las pruebas.
SIELE certifica el grado de competencia en la lengua española a través de cuatro pruebas: Comprensión de lectura, Comprensión auditiva, Expresión e interacción escritas y Expresión e interacción orales; y toma como referencia los niveles del Marco común europeo de referencia para las lenguas (MCER). Las dos primeras pruebas se califican automáticamente por el sistema informático, mientas que las expresiones son corregidas por calificadores autorizados, aplicando estrictos criterios de evaluación.
Se trata de un único examen multinivel que permite obtener un certificado SIELE, documento que incluye una puntuación de 0 a 1000, resultado de la suma de la puntuación de cada una de las cuatro pruebas. El empleo de la Tecnologías de la Información y la Comunicación (TIC) permite disponer de un examen ágil y flexible.
Uso de plantas para fins cosméticos por mulheres: uma alternativa sustentável
Authors: Costa Ana Paula , Jesus Fernanda , Marisco Marisco 
Published: February 2022

O objetivo deste estudo foi identificar, por um levantamento etnobotânico, o comportamento de compra e frequência de uso de cosméticos, bem como as plantas utilizadas para esses fins. Essa pesquisa foi realizada por meio de questionários on-line destinado exclusivamente às mulheres. O questionário foi respondido por 59 mulheres com idade entre 18 e 30 anos. A maioria (77,2%) afirma que a internet, amigos e familiares são as principais influências para a escolha dos produtos que utilizam, sendo os produtos para o cabelo, higiene corporal e íntima os mais usados. As plantas acerola, uva, abacaxi, morango e caju foram as frutas reconhecidas com ação anti-idade contribuindo com a pele. A partir dessa pesquisa, foi possível observar que as mulheres carregam conhecimento cultural sobre plantas, entretanto muitos desses conhecimentos são a partir de redes sociais. Nessa perspectiva, esse saber não garante eficiência, tornando necessário o desenvolvimento de estudos que confirmem essa percepção popular. Infere-se a necessidade de estudos a fim de proporcionar segurança na sua utilização tanto para os consumidores como para o meio ambiente, seja na sua forma in natura ou em associação com cosméticos, associado com a preservação da biodiversidade de forma sustentável pode contribuir nas relações de saúde única.