bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of pollution risks posed by trace elements in the riverbed sediment of the Ganges River in Eastern Uttar Pradesh, India
Authors: Aggarwal Mohit , Anbukumar Srinivasan , Vijaya Kumar Thangamani 
Published: February 2025

ABSTRACT

This research investigates trace elements in newly deposited riverbed sediment along a 225-km section of the Ganges River in eastern Uttar Pradesh, India. Samples were collected from 10 locations between Kanpur and Prayagraj, and were analyzed using atomic absorption spectrometry (AAS). The study focused on eight heavy metals: iron (Fe), manganese (Mn), cadmium (Cd), copper (Cu), chromium (Cr), nickel (Ni), lead (Pb), and zinc (Zn). The pollution risk was assessed using four indicators: enrichment factor (EF), Geo-Accumulation Index (GAI), contamination factor (CF), and Pollution Load Index (PLI). Fe (24,288 ppm) had the highest average concentration, followed by Mn (488.9 ppm), Cr (52.36 ppm), Zn (29.75 ppm), Ni (29.71 ppm), Pb (23.36 ppm), Cu (22.95 ppm), and Cd (1.10 ppm). Average values of CF indicated moderate contamination by Pb (1.06) and Cd (1.89). The average EF values for Cd at 3.15 showed moderate enrichment (EF between 2 and 5). GAI values indicated “uncontaminated to moderate” contamination (GAI between 0 and 1) by Cd (0.30), posing potential ecological risks. PLI values suggested baseline sediment contamination (PLI &lt; 1) across all sites. The study highlights that Cd and Pb levels are concerning and may worsen with increased human activity in the river's catchment area.
Evaluation of Antiseptic Drug Candidate Pyridosept in Preclinical in Vitro Studies
Authors: Kazakova Renata , Iksanova Alfiya , Agafonova Mariya , Lubina Anna , Bulatova Elena , Nikitina Elena , Lisovskaya Svetlana , Kayumov Airat , Bondar Oxana , Malanyeva Albina , Vasileva Olga , Zeldi Marina , Gatina Alina , Koshkin Sergei , Komcharova Natalia , Konyakhina Yulia , Titova Kseniia , Sergeev Aleksandr , Sapozhnikov Sergey , Shtyrlin Nikita , Shtyrlin Yurii 
Published: February 2025

Introduction:
This work provides preclinical in vitro studies of an innovative
antimicrobial agent named pyridosept, belonging to the quaternary bis-ammonium salts
and synthesized on the base of pyridoxine. Since the wide spread of pathogens with tolerance
to both antibiotics and antiseptics challenges the development of new antimicrobials
providing high efficiency against fungi and microorganisms with multiple resistance.

Method:
This work presents in vitro studies of pyridosept, such as antibacterial and antifungal
activities (determination of minimal inhibitory concentration and quantitative suspension
test method), as well as antibacterial activity of metal surface test, sporicidal
and spermicidal activities, formation of resistance (to bacteria and fungi), Skin-PAMPA
(study of membrane transdermal permeability), mutagenicity assays (in Ames test and
SOS-chromotest), cytotoxicity (MTT-assay) and comet assay in human skin fibroblasts.

Results:
Pyridosept demonstrates advantageous antibacterial and antifungal activity in
both suspension and metal surface tests; it has anti-SARS-CoV-2, spermicidal and sporicidal
activities (comparable to chlorhexidine digluconate). On the other hand, it demonstrates
a low frequency of bacterial resistance development (no more than 2-4-fold increase
of the MIC in 30 passages, with MIC values fluctuating within 1-8 μg/ml for
Gram-positive bacteria and 4-32 μg/ml for Gram-negative bacteria) and membrane transdermal
permeability (Skin-PAMPA, 500-fold less compared to progesterone). While cytotoxicity
(in MTT- and comet assay) of pyridosept is comparable with those for bezalkonium
chloride and chlorhexidine, our results suggest that pyridosept represents an advantageous
structure for the design of new antiseptics.

Conclusion:
The information obtained during the studies demonstrated that pyridosept
has a good efficacy and safety profile, which allows us to consider it as a potential antiseptic
and disinfectant, for which it is necessary to conduct additional in vivo studies.
DDX59-AS1: A Novel Prognostic Biomarker and Immunotherapy Predictor in Lung Adenocarcinoma
Authors: Wang Yanli , Li Wei , Wei Su , Zhang Lixi , Li Dongbing , Qi Xu 
Published: February 2025

Background:
The precise function of DDX59 Antisense RNA 1 (DDX59-
AS1) in lung adenocarcinoma (LUAD) has yet to be fully elucidated.

Objective:
This study uses bioinformatics analysis and experimental validation to investigate
the association between DDX59-AS1 and LUAD.

Methods:
This study uses statistical analysis and database interrogation to investigate
the potential association between DDX59-AS1 expression and various clinical characteristics,
prognostic factors, regulatory networks, and immune infiltration in LUAD. The
quantification of DDX59-AS1 expression in LUAD cell lines is conducted through the
use of quantitative real-time polymerase chain reaction (qRT-PCR).

Results:
DDX59-AS1 showed significantly elevated levels of expression in patients
with LUAD. High levels of DDX59-AS1 expression were found to be significantly associated
with poorer overall survival (OS) in patients with LUAD (p = 0.024). Furthermore,
an independent correlation was observed between high DDX59-AS1 expression
(p = 0.037) and OS in LUAD patients. DDX59-AS1 was found to be involved in various
pathways, including glutathione metabolism, proteasome function, and the cytosolic
DNA sensing pathway, among others. A significant correlation was observed between
the expression levels of DDX59-AS1 and immune cell infiltration in the context of LUAD.
Notably, elevated expression of DDX59-AS1 was observed in LUAD cell lines
compared to the non-cancerous Beas-2B cell line.

Conclusion:
A significant correlation was observed between elevated DDX59-AS1 expression
in patients with LUAD and adverse prognosis, alongside increased immune infiltration.
These results indicate that DDX59-AS1 may function as a prognostic marker
for LUAD and a potential predictor of immunotherapy response.
Chloride Intracellular Channel 1 Enhances Glioblastoma Cell Migration and Epithelial-Mesenchymal Transition by Activating the ERK1/2 Signaling Pathway
Authors: Zhang Kai , Wu Yue , Han Lin , Miao Xingyu 
Published: February 2025

Background:
Glioblastoma is a common primary malignant intracranial tumor in
adults associated with high disability and mortality. Despite the use of traditional surgical methods,
postoperative radiotherapy, and targeted therapies, the median survival for glioma patients remains
disappointingly brief. As a result, there is an urgent need to explore new targets and develop
novel targeted drugs to potentially improve patient survival. Notably, CLIC1 expression is upregulated
in tumors and correlated to tumor aggressiveness, metastasis, and poor prognosis.
Nonetheless, its potential role in gliomas remains largely unclear.

Objective:
This study aimed to investigate the bioinformatics characteristics and clinicopathological
features of CLIC1, including WHO classification and OS.

Methods:
Immunohistochemistry and western blot analysis were carried out to detect the expression
of CLIC1 in glioma tissues. Moreover, CCK8, plate clone formation assay, and EdU proliferation
assay were carried out for cell proliferation ability. Transwell and scratch assay were performed
for cell invasion and migration. Western blotting was also conducted to verify the relationship
between CLIC1 and EMT and ERK1/2 signaling pathway. The effect of the knockdown of
CLIC1 on tumor growth capacity was assessed in an intracranial xenograft model.

Results:
CLIC1 was found to be associated with poor prognosis in glioma patients, and in vivo experiments
demonstrated that CLIC1 promoted GBM cell proliferation, invasion, and migration. In
addition, CLIC1 positively regulated ERK1/2 signaling to promote the EMT process in GBM
cells. In vitro experiments showed that CLIC1 could affect intracranial tumor progression in mice.

Conclusion:
In summary, these findings expand our knowledge of CLIC1, confirming its oncogenic
role and laying the groundwork for future development of pharmacological agents targeting
this gene.
Microglial Modulation in Alzheimer’s Disease: Central Players in Neuroinflammation and Pathogenesis
Authors: Hussain Md Sadique , Khan Yumna , Fatima Rabab , Maqbool Mudasir , Ramalingam Prasanna Srinivasan , Khan Mohammad Gayoor , Bisht Ajay Singh 
Published: February 2025

Abstract:
Alzheimer’s disease (AD) is an age-related, progressive neurodegenerative disorder of
cognition with clinical features and anatomical hallmarks of amyloid-β plaques and/or neurofibrillary
tangles. New studies revealed that microglia, the native immune cells in the brain, are crucial
in the development of AD. The present review aims at outlining various roles of microglia in AD
especially targeting their role in neuroinflammation. These indicate that microglial dysfunction
contributes to AD pathology by affecting both amyloid-β phagocytosis and tau hyperphosphorylation.
Other investigative molecular perpetrators, including TREM2, also influence the microglial
relevance to amyloid and tau, as well as the overall disease phase. The functional microglia can
protect neurons, while the dysfunctional one has the capability of derailing neuronal potentials
and aggravating neurodegeneration. We have also discussed therapeutic strategies that start with
targeting microglia to reduce neuroinflammation and reinstate balance. However, certain
problems, including the side effects of microglial modulation, cost constraint, and accessibility,
are areas of concern. In this review, the author presents the current state of knowledge on the potential
of microglia-targeted treatments, their risks, and benefits. Thus, this article emphasizes the
importance of the expansion of research to decipher the exact manipulation of microglia in AD
with the goal of applying these findings given therapeutic approaches.
Evaluation of Cardiovascular and Hepatic Changes in Myocardial Infarction Patients Post-Covid-19 Vaccination
Authors: Mehanna Mohamed Gamil , Eid Thamir Mahmoud , Maarof Badr Abdullah , Baig Mirza Rafi , Naqvi Salma , Alabassi Fahad A. , El gayar Ahmed El Sayed , Omar Abdelmaaboud M.M. , Al-Bar Omar A. , Gazi Shaikh , Kumar Vikas , Anwar Firoz 
Published: February 2025

Introduction:
The global COVID-19 vaccination campaign has significantly reduced severe
illness and mortality; however, emerging evidence raises concerns regarding its potential cardiovascular
effects, particularly myocardial infarction (MI).

Method:
This study investigates the relationship between COVID-19 vaccination and MI incidence
among first-time MI patients in Saudi Arabia. Post-COVID-19 vaccination within six months postvaccination
accounted for potential confounding factors, such as pre-existing health conditions, age,
and lifestyle. A total of 102 MI patients, with a male predominance of 60.8% and a significant correlation
with middle age, were analysed. A+ blood group patients were the most prevalent (33.3%),
followed by B+ (29.4%), while Rh-negative patients constituted only 7.8%. Elevated mean BNP
(761.98 pg/ml), pulse rate (87.72 bpm), and systolic blood pressure (139.98 mmHg) indicated heightened
cardiac stress (p < 0.01).

Results:
Significant elevations in AST (121.65 U/L) and ALT (133.63 U/L) levels suggested liver
stress post-Covid-19 vaccination (p < 0.01). Males had higher AST, ALT, and bilirubin levels than
females, with p-values of 0.02, 0.01, and 0.04, respectively, indicating hepatic differences. Elevated
biomarkers like CK-MB (58.05 IU/L) and CPK (313.86 mcg/L) further affirmed significant myocardial
damage post-vaccination (p < 0.05).

Conclusion:
These findings suggest a link between vaccination and cardiovascular events and highlight
the importance of considering individual health profiles in evaluating vaccine safety, cardiovascular
health, and hepatic implications.
Optimization of Formulation and Physicochemical, Sensory and Nutritional Evaluation of Shelf-stable Dehydrated Ready-To-Eat Salads
Authors: Koli Rachana , Prasuna R. Gyana 
Published: February 2025

Background:
In the fast-paced world of today, "time" is a valuable and limited resource
that can make a difference in our lives. Furthermore, since more people are employed,
there is a greater need for ready-to-eat foods. Salad is an essential component of the daily diet.

Objectives:
The main objective of this study was to develop a nutrient-dense, dehydrated readyto-
eat salad with dehydrated fruits (pineapple), vegetables (beetroot, carrot, and pumpkin seed),
and salad dressings.

Methods:
In this work, development of four salad dressings with different flavours and aromas
has been carried out, namely honey mustard dressing (A), tahini dressing (B), Caesar dressing
(C), and balsamic vinaigrette dressing (D) to improve the palatability of salad. All 16 variations
were subjected to a sensory examination utilizing a nine-point hedonic scale. A
physicochemical investigation was additionally carried out on the variants that received good
acceptance ratings. Total Plate Count Test was used to determine the product's shelf life.

Results:
The study's findings indicate that the Variation 4C variant's sensory qualities were
well-received, receiving an overall consumer acceptability rating of 8.83. The ratios of carrot,
beetroot, pumpkin seed, pineapple, and Caesar dressing were 25%:15%:15%:15%:30%,
respectively. The dehydrated RTE salad (Variation 4C) variation has 20.98% moisture, 15.27 g
protein, 10.10 g fat, 4.84 g crude fiber, 6.20mg of minerals, and 3.3mg of vitamin A/100g.
Microbiologically, V4C variation was acceptable for up to 60 days when refrigerated at 8°C.

Conclusion:
Overall research findings indicate that consumers accept salad in dehydrated
ready-to-eat salad form, offered in different combinations, while retaining its nutritional value.
In Silico Identification of Emblica officinalis Compounds Inhibiting Thermolabile Hemolysin from Vibrio alginolyticus in Shrimp
Authors: Bari Sayed Mashequl , Marma Meamaching , Reza Nafees Bin , Ahmed Sk. Faisal , Shoriful Islam Md. , Rayhan Nafis , Hossian Md. Alomgir , Matiur Rahman Md. , Saiful Alam Md. 
Published: February 2025

Background:
Thermolabile hemolysin (TLH) is a key virulent protein of Vibrio alginolyticus,
known for its hemolytic and phospholipase activities, leading to shrimp vibriosis disease.
It has been suggested as a potential therapeutic candidate for vibriosis therapy.

Methods:
Computational studies, including molecular docking, toxicity analysis, and molecular
dynamics (MD) simulations, were conducted to investigate the inhibition of the phospholipase
activity of TLH by phytochemicals from Emblica officinalis.

Results:
Out of the twenty-nine compounds, the top three, including Ellagic acid (CID
5281855), Quercetin (CID 5280343), and Kaempferol (CID 5280863), were sorted based on
their highest molecular docking scores of -9.2, -8.9, and -8.8, respectively. Subsequently, molecular
dynamics (MD) simulations of these selected leads were performed to observe the structural
stability of these compounds in the binding sites of TLH protein. The MD simulation outcomes
indicated that all three compounds demonstrated superior stability throughout 100 nanoseconds
compared to the control compound Resveratrol. The molecular simulation results suggest
stable interactions, with average root-mean-square deviation (RMSD) and root-meansquare
fluctuation (RMSF) values of 1-2 Å and 0-3 Å. Pharmacokinetic and toxicity analyses
were conducted to evaluate the suitability and toxicity of these selected compounds. All top
three compounds passed the Lipinski rule, and toxicity criteria.

Conclusion:
Therefore, these compounds have the potential to serve as effective therapeutics
for controlling Vibrio alginolyticus infection in shrimp.
Validity and Reliability of the Tunisian Arabic Functioning Assessment Short Test (FAST) and Functional Outcome Factors in a Sample of Patients with Bipolar Disorder
Authors: Jemli Hend , Ouali Uta , Maatouk Ons , Hajri Ahlem , Ahmed Azza Ben Cheikh , Aissa Amina , Jomli Rabaa 
Published: February 2025

Background
Bipolar disorders negatively impact functional outcomes and, consequently, prognosis. The Functioning Assessment Short Test is a reliable tool to evaluate functional outcomes in people with bipolar disorders.

Aim
The aim of the study was to conduct a cross-cultural validation of the Functioning Assessment Short Test (FAST) and to explore correlations between functional impairment and sociodemographic and clinical variables.

Methods
A cross-sectional study was carried out in a population of 60 bipolar patients and 60 healthy controls. The scales administered were the Global Functioning Assessment (GAF), the World Health Organization Quality Of Life-Bref (WHOQOL-BREF), the FAST, and a questionnaire containing sociodemographic and clinical variables. The validation study was based on face and content validity, reliability, and construct validity.

Results
The face and content validity were satisfactory. The internal consistency obtained was high, with a Cronbach’s alpha of 0.785. All six FAST domains had significant correlations with each other and with the total score. The FAST assessment at baseline and week 2 were highly correlated (p>0.05), and the intraclass correlation coefficient was 0.998, indicating high test-retest reliability. The FAST total score was negatively and significantly associated with GAF (rho=-0.788, p<0.001) and WHOQOL-BREF scores, suggesting good concurrent validity. The total FAST scores were significantly lower in controls as compared with bipolar patients (p<0.001), with a cut-off at 26. Functional impairment was significantly associated with the following variables: low educational level, living alone, early age at onset, number of depressive episodes, and treatment associations (mood stabilizers and antipsychotics).

Conclusion
The Tunisian Arabic version of the FAST demonstrated satisfactory psychometric properties and could be used to assess specific domains of functional impairment in people living with bipolar disorders and may be instrumental in implementing psychosocial and rehabilitation interventions.
Effect of Levothyroxine Therapy on Gestational Hypertension and Pre-Eclampsia in Pregnant Women with Subclinical Hypothyroidism, Hypothyroidism, and Thyroid Autoimmunity: A Systematic Review and Meta-analysis
Authors: Goodarzi-Khoigani Masoomeh , Aminorroaya Ashraf , Mohammadi Raziyeh 
Published: February 2025

Background:
Gestational hypertension (GH) and preeclampsia (PE) are two
important complications of pregnancy. Considering the U-shaped association between thyroidstimulating
hormone (TSH) and hypertensive disorders of pregnancy in some reports, we decided
to investigate the effect of levothyroxine treatment on GH and PE in pregnant women
with subclinical hypothyroidism (SGH), overt hypothyroidism (OH), and autoimmune thyroid
diseases.

Methods:
Google Scholar and databases, such as ProQuest, Medline, Cochrane Library, ScienceDirect,
and Scopus were searched electronically for clinical trials and observational studies
using the following search terms: (("levothyroxine" OR "LT4" OR "thyroxine supplementation")
AND ("subclinical hypothyroidism" OR "SCH" OR "thyroid peroxidase antibodies" OR "autoimmune
thyroid disease") AND ("pregnancy outcomes" OR "preeclampsia" OR "gestational hypertension"
OR "PIH")). Further, we investigated the impact of levothyroxine on the incidence
of GH and/or PE compared with control or placebo groups from April 4 to November 1, 2022.

Results:
After treatment with levothyroxine, the odd ratios (ORs) of GH and PE in subclinical
[OR = 1.03, 95% CI: (0.85, 1.25), I2 = 35.25%, P =0.78, OR = 1.02, 95% CI: (0.66,1.58), I2 =
46.86%, P =0.94, respectively] and overt hypothyroidism [OR=1.10, 95% CI: (0.70,1.71),
I2=38.44%, P =0.69, OR=1.32, 95% CI: (0.83, 2.09), I2=0.00%, P =0.24, respectively] were not
different from controls. Furthermore, this result was observed in studies that recruited women
with SCH and OH [OR=1.12, 95% CI: (0.58, 2.14), I2=92.74%, P =0.74, OR=0.51, 95% CI:
(0.15, 1.72), I2=97.30%, P =0.28, respectively]. Also, these studies compared thyroperoxidase
antibodies between TPOAb-positive participants with TPOAb-negative controls (OR=1.01, 95%
CI: (0.80, 1.28), I2=0.00%, P =0.90). However, LT4 reduced the risk of GH in TPOAb+ women
compared with untreated TPOAb+ (OR=0.43, 95% CI: (0.30, 0.62), I2=0.00%, P =0.00).

Conclusion:
After LT4 therapy, the incidence rates of GH and PE in any form of hypothyroidism
were not significantly different from controls. However, the reduction of GH in women with
TPOAb+ who used levothyroxine needs further consideration.
A Comparative Analysis of COVID-19-Associated and Non-COVID-19-Associated Mucormycosis
Authors: Kömür Süheyla , Candevira Aslıhan , İnala Ayşe Seza , Kuşcua Ferit , Kurtarana Behice , Memişoğlub Funda , Karaoğlanc İlkay , Taşovaa Yeşim 
Published: February 2025

Background:
COVID-19-associated Mucormycosis (CAM) has emerged as a significant
complication during the COVID-19 pandemic. However, there is a lack of comprehensive
comparative studies with non-COVID-associated mucormycosis (NCM).

Objective:
This study aims to compare the clinical characteristics, risk factors, and outcomes of
CAM and NCM to enhance the understanding and management of these infections, particularly
during the COVID-19 pandemic.

Method:
A retrospective multicenter study was conducted at Cukurova University, Malatya İnönü
University, and Gaziantep University. We analyzed and compared cases of CAM and NCM diagnosed
between January 2018 and February 2022. Data were collected from the infectious diseases
and clinical microbiology departments, including demographic details, underlying conditions,
treatment regimens, and outcomes.

Results:
A total of 38 cases were analyzed, with 21 cases of COVID-19-associated mucormycosis
(CAM) and 17 cases of non-COVID-19-associated mucormycosis (NCM). The key findings of the
study were as follows: CAM was strongly associated with corticosteroid use (p<0.001) and diabetes
(p=0.001), while NCM cases were more frequently linked to malignancy and neutropenia
(p<0.05). Clinically, CAM cases had a higher incidence of cavernous sinus involvement and bone
destruction (p=0.003) compared to NCM cases. However, there was no significant difference in
overall survival between the CAM and NCM groups (p=0.201).

Conclusion:
The study highlights the critical role of corticosteroid use and diabetes as prominent
risk factors for CAM. Timely diagnosis and intervention are essential to prevent severe complications,
such as cavernous sinus involvement and bone destruction. These findings emphasize the
need for tailored management strategies for CAM in the context of COVID-19, with particular attention
to these risk factors.