bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathological characteristics and outcomes of malignant adenomyoepithelioma of the breast: a single institution’s experience
Authors: Alqudaihi Heba Mohammad Abdulla , Lee Sae Byul , Son Byung Ho , Ahn Sei-Hyun , Lee Jong Won , Ko Beom Seok , Kim Hee Jeong , Chung Il Yong , Kim Jisun , Gong Gyungyub 
Published: April 2022

Abstract
Background
Malignant adenomyoepithelioma of the breast is a rare tumor and most of relevant literature consists of individual case reports. This study objective was designed to evaluate clinicopathological features and treatment outcomes of 15 cases of malignant adenomyoepithelioma at a single institute.

Methods
A retrospective medical record review was performed for 15 subjects confirmed with malignant adenomyoepithelioma upon postoperative pathological diagnosis at the Asan Medical Center from January 2008 to June 2018. Data regarding age at diagnosis, preoperative biopsy results, operation methods, the status of hormone receptors and HER2, and clinical outcomes were collected.

Results
All cases were female patients diagnosed at median age of 50 years. Preoperative core needle biopsy results showed that 40% of the cases (6 out of 15) were benign which was in discordance with the final malignant pathology report. Thirteen cases underwent wide excision with or without sentinel lymph node biopsy (SLNB) and 2 cases had total mastectomy with SLNB. Five of 11 cases (45.5%) were triple negative. Ten of 15 cases underwent postoperative radiation therapy, 3 cases underwent chemotherapy, and 5 cases underwent endocrine therapy. During median follow-up of 55 months, the 5-year overall survival rate was 87.5% and the 5-year disease free survival rate was 91.7%. Two lung metastases developed. One case showed local recurrence 3 years after surgery and radiotherapy and subsequently developed lung metastasis 1 year late. Another case developed lung metastasis one and a half years after surgery in combination with endocrine therapy and neoadjuvant chemotherapy.

Conclusion
Preoperative core needle biopsy showed inaccurate results for diagnosing malignant adenomyoepithelioma. Malignant adenomyoepithelioma has a high rate of triple negative subtype but has a relatively good prognosis although there is a risk of local and systemic recurrence.
Assessing differences in contraceptive provision through telemedicine among reproductive health providers during the COVID-19 pandemic in the United States
Authors: Comfort Alison B. , Rao Lavanya , Goodman Suzan , Raine-Bennett Tina , Barney Angela , Mengesha Biftu , Harper Cynthia C. 
Published: April 2022

AbstractBackgroundProviders faced challenges in maintaining patient access to contraceptive services and public health safety during the COVID-19 pandemic. Due to increased barriers to care, providers increasingly used telemedicine for contraceptive care, curbside services, mail-order pharmacies, and on-line or home delivery of contraceptive methods, including self-administration of subcutaneous depo medroxyprogesterone acetate (DMPA-SQ). To better understand how reproductive health providers adapted service provision during the pandemic, this study assessed clinical practice changes and strategies providers adopted throughout the United States to maintain contraceptive care, particularly when clinics closed on-site, and the challenges that remained in offering contraceptive services, especially to marginalized patient populations.MethodsWe surveyed U.S. providers and clinic staff (n = 907) in April 2020–January 2021, collecting data on contraceptive service delivery challenges and adaptations, including telemedicine. We assessed clinical practice changes with multivariate regression analyses using generalized linear models with a Poisson distribution and cluster robust standard errors, adjusting for clinic patient volume, practice setting, region, Title X funding, and time of survey.ResultsWhile 80% of providers reported their clinic remained open, 20% were closed on-site. Providers said the pandemic made it more difficult to offer the full range of contraceptive methods (65%), contraceptive counseling (61%) or to meet the needs of patients in marginalized communities (50%). While only 11% of providers offered telemedicine pre-pandemic, most offered telemedicine visits (79%) during the pandemic. Some used mail-order pharmacies (35%), curbside contraceptive services (22%), and DMPA-SQ for self-administration (10%). Clinics that closed on-site were more likely to use mail-order pharmacies (aRR 1.83, 95% CI [1.37–2.44]) and prescribe self-administered DMPA-SQ (aRR 3.85, 95% CI [2.40–6.18]). Clinics closed on-site were just as likely to use telemedicine as those that remained open. Among clinics using telemedicine, those closed on-site continued facing challenges in contraceptive service provision.ConclusionsClinics closing on-site were just as likely to offer telemedicine, but faced greater challenges in offering contraceptive counseling and the full range of contraceptive methods, and meeting the needs of marginalized communities. Maintaining in-person care for contraceptive services, in spite of staffing shortages and financial difficulties, is an important objective during and beyond the pandemic.
Altered gene expression of VEGF, IGFs and H19 lncRNA and epigenetic profile of H19-DMR region in endometrial tissues of women with endometriosis
Authors: Kamrani Sedigheh , Amirchaghmaghi Elham , Ghaffari Firouzeh , Shahhoseini Maryam , Ghaedi Kamran 
Published: April 2022

Abstract
Background
Endometriosis, as chronic estrogen-dependent disease, is defined by the presence of endometrial-like tissue outside the uterus. Proliferation of endometrial tissue and neoangiogenesis are critical factors in development of endometriosis. Hence, vascular endothelial growth factor (VEGF) as well as insulin‐like growth factor 1 and 2 (IGF1, 2) may be involved as inducers of cellular proliferation or neoangiogenesis. Imprinted long noncoding RNA H19 (lncRNA H19) has been suggested to be involved in pathogenesis of endometriosis via regulation of cellular proliferation and differentiation. Epigenetic aberrations appear to play an important role in its pathogenesis. The present study was designed to elucidate VEGF, IGF1, IGF2 and H19 lncRNA genes expression and epigenetic alterations of differentially methylated region (DMR) of H19 (H19-DMR) regulatory region in endometrial tissues of patients with endometriosis, in comparison with control women.

Methods
In this case–control study, 24 women with and without endometriosis were studied for the relative expression of VEGF, IGF1, IGF2 and H19 lncRNA genes using real-time polymerase chain reaction (PCR) technique. Occupancy of the MeCP2 on DMR region of H19 gene was assessed using chromatin immunoprecipitation (ChIP), followed by real-time PCR.

Results
Genes expression profile of H19, IGF1 and IGF2 was decreased in eutopic and ectopic endometrial tissues of endometriosis group, compared to the control tissues. Decreased expression of H19 in ectopic samples was significant in comparison with the controls (P < 0.05). Gene expression of VEGF was increased in eutopic tissues of endometriosis group, compared to control group. Whereas its expression level was lower in ectopic lesions versus eutopic and control endometrial samples. ChIP analysis revealed significant and nearly significant hypomethylation of H19-DMR region II in eutopic and ectopic samples, compared to the control group respectively. This epigenetic change was aligned with expression of IGF2. While methylation of H19-DMR region I was not significantly different between the eutopic, ectopic and control endometrial samples.

Conclusion
These data showed that VEGF, IGF1, IGF2 and H19 lncRNA genes expression and epigenetic alterations of H19 lncRNA have dynamic role in the pathogenesis of endometriosis, specifically in the way that hypomethylation of H19-DMR region II can be involved in IGF2 dysregulation in endometriosis.
Settings matter: a scoping review on parameters in robot-assisted gait therapy identifies the importance of reporting standards
Authors: van Dellen Florian , Labruyère Rob 
Published: April 2022

Abstract
Background
Lokomat therapy for gait rehabilitation has become increasingly popular. Most evidence suggests that Lokomat therapy is equally effective as but not superior to standard therapy approaches. One reason might be that the Lokomat parameters to personalize therapy, such as gait speed, body weight support and Guidance Force, are not optimally used. However, there is little evidence available about the influence of Lokomat parameters on the effectiveness of the therapy. Nevertheless, an appropriate reporting of the applied therapy parameters is key to the successful clinical transfer of study results. The aim of this scoping review was therefore to evaluate how the currently available clinical studies report Lokomat parameter settings and map the current literature on Lokomat therapy parameters.

Methods and results
A systematic literature search was performed in three databases: Pubmed, Scopus and Embase. All primary research articles performing therapy with the Lokomat in neurologic populations in English or German were included. The quality of reporting of all clinical studies was assessed with a framework developed for this particular purpose. We identified 208 studies investigating Lokomat therapy in patients with neurologic diseases. The reporting quality was generally poor. Less than a third of the studies indicate which parameter settings have been applied. The usability of the reporting for a clinical transfer of promising results is therefore limited.

Conclusion
Although the currently available evidence on Lokomat parameters suggests that therapy parameters might have an influence on the effectiveness, there is currently not enough evidence available to provide detailed recommendations. Nevertheless, clinicians should pay close attention to the reported therapy parameters when translating research findings to their own clinical practice. To this end, we propose that the quality of reporting should be improved and we provide a reporting framework for authors as a quality control before submitting a Lokomat-related article.
Assessment of the toxicity and carcinogenicity of double-walled carbon nanotubes in the rat lung after intratracheal instillation: a two-year study
Authors: Saleh Dina Mourad , Luo Shengyong , Ahmed Omnia Hosny Mohamed , Alexander David B. , Alexander William T. , Gunasekaran Sivagami , El-Gazzar Ahmed M. , Abdelgied Mohamed , Numano Takamasa , Takase Hiroshi , Ohnishi Makoto , Tomono Susumu , Hady Randa Hussein Abd el , Fukamachi Katsumi , Kanno Jun , Hirose Akihiko , Xu Jiegou , Suzuki Shugo , Naiki-Ito Aya , Takahashi Satoru , Tsuda Hiroyuki 
Published: April 2022

Abstract
Background
Considering the expanding industrial applications of carbon nanotubes (CNTs), safety assessment of these materials is far less than needed. Very few long-term in vivo studies have been carried out. This is the first 2-year in vivo study to assess the effects of double walled carbon nanotubes (DWCNTs) in the lung and pleura of rats after pulmonary exposure.

Methods
Rats were divided into six groups: untreated, Vehicle, 3 DWCNT groups (0.12 mg/rat, 0.25 mg/rat and 0.5 mg/rat), and MWCNT-7 (0.5 mg/rat). The test materials were administrated by intratracheal-intrapulmonary spraying (TIPS) every other day for 15 days. Rats were observed without further treatment until sacrifice.

Results
DWCNT were biopersistent in the rat lung and induced marked pulmonary inflammation with a significant increase in macrophage count and levels of the chemotactic cytokines CCL2 and CCL3. In addition, the 0.5 mg DWCNT treated rats had significantly higher pulmonary collagen deposition compared to the vehicle controls. The development of carcinomas in the lungs of rats treated with 0.5 mg DWCNT (4/24) was not quite statistically higher (p = 0.0502) than the vehicle control group (0/25), however, the overall incidence of lung tumor development, bronchiolo-alveolar adenoma and bronchiolo-alveolar carcinoma combined, in the lungs of rats treated with 0.5 mg DWCNT (7/24) was statistically higher (p < 0.05) than the vehicle control group (1/25). Notably, two of the rats treated with DWCNT, one in the 0.25 mg group and one in the 0.5 mg group, developed pleural mesotheliomas. However, both of these lesions developed in the visceral pleura, and unlike the rats administered MWCNT-7, rats administered DWCNT did not have elevated levels of HMGB1 in their pleural lavage fluids. This indicates that the mechanism by which the mesotheliomas that developed in the DWCNT treated rats is not relevant to humans.

Conclusions
Our results demonstrate that the DWCNT fibers we tested are biopersistent in the rat lung and induce chronic inflammation. Rats treated with 0.5 mg DWCNT developed pleural fibrosis and lung tumors. These findings demonstrate that the possibility that at least some types of DWCNTs are fibrogenic and tumorigenic cannot be ignored.
RETRACTED ARTICLE: microRNA-15b-5p encapsulated by M2 macrophage-derived extracellular vesicles promotes gastric cancer metastasis by targeting BRMS1 and suppressing DAPK1 transcription
Authors: Cao Yi , Tu Yi , Xiong Jianbo , Tan Shengxing , Luo Lianghua , Wu Ahao , Shu Xufeng , Jie Zhigang , Li Zhengrong 
Published: April 2022

Abstract
Background
Extracellular vesicles (EVs) derived from tumor-associated macrophages are implicated in the progression and metastasis of gastric cancer (GC) via the transfer of molecular cargo RNAs. We aimed to decipher the impact of microRNA (miR)-15b-5p transferred by M2 macrophage-derived EVs in the metastasis of GC.

Methods
Expression of miR-15b-5p was assessed and the downstream genes of miR-15b-5p were analyzed. GC cells were subjected to gain- and loss-of function experiments for miR-15b-5p, BRMS1, and DAPK1. M2 macrophage-derived EVs were extracted, identified, and subjected to co-culture with GC cells and their biological behaviors were analyzed. A lung metastasis model in nude mice was established to determine the effects of miR-15b-5p on tumor metastasis in vivo.

Results
miR-15b-5p was upregulated in GC tissues and cells as well as in M2 macrophage-derived EVs. miR-15b-5p promoted the proliferative and invasive potentials, and epithelial-mesenchymal transition (EMT) of GC cells. M2 macrophage-derived EVs could transfer miR-15b-5p into GC cells where it targeted BRMS1 by binding to its 3’UTR. BRMS1 was enriched in the DAPK1 promoter region and promoted its transcription, thereby arresting the proliferative and invasive potentials, and EMT of GC cells. In vivo experiments demonstrated that orthotopic implantation of miR-15b-5p overexpressing GC cells in nude mice displayed led to enhanced tumor metastasis by inhibiting the BRMS1/DAPK1 axis.

Conclusions
Overall, miR-15b-5p delivered by M2 macrophage-derived EVs constitutes a molecular mechanism implicated in the metastasis of GC, and may thus be considered as a novel therapeutic target for its treatment.
The Notch signaling network in muscle stem cells during development, homeostasis, and disease
Authors: Gioftsidi Stamatia , Relaix Frederic , Mourikis Philippos 
Published: April 2022

AbstractSkeletal muscle stem cells have a central role in muscle growth and regeneration. They reside as quiescent cells in resting muscle and in response to damage they transiently amplify and fuse to produce new myofibers or self-renew to replenish the stem cell pool. A signaling pathway that is critical in the regulation of all these processes is Notch. Despite the major differences in the anatomical and cellular niches between the embryonic myotome, the adult sarcolemma/basement-membrane interphase, and the regenerating muscle, Notch signaling has evolved to support the context-specific requirements of the muscle cells. In this review, we discuss the diverse ways by which Notch signaling factors and other modifying partners are operating during the lifetime of muscle stem cells to establish an adaptive dynamic network.
Dynamical analysis of a stochastic delayed SIR epidemic model with vertical transmission and vaccination
Authors: Zhang Xiaolei , Liu Maoxing 
Published: April 2022

AbstractIn order to describe the dynamic process of epidemic transmission with vertical transmission and vaccination in more detail and to better track the factors that lead to the occurrence of epidemics, we construct a stochastic delayed model with a specific functional response to describe its epidemic dynamics. We first prove the existence and uniqueness of the positive solution of the model. Moreover, we analyze the sufficient conditions for the extinction and persistence of the model. Finally, numerical simulations are presented to illustrate our mathematical findings.
A comprehensive review on oleaginous bacteria: an alternative source for biodiesel production
Authors: Koreti Deepali , Kosre Anjali , Jadhav Shailesh Kumar , Chandrawanshi Nagendra Kumar 
Published: April 2022

AbstractDue to continuously increasing population, industrialization, and environmental pollution, lead to generating high energy demand which suitable for our environment. Biodiesel is an alternative renewable fuel source. According to the feedstock of production, biodiesel has been categorized into four generations. The main disadvantage of the first and second generation is the raw material processing cost that the challenge for its industrial-level production. Oleaginous bacteria that contain more than 20% lipid of their cellular biomass can be a good alternative and sustainable feedstock. Oleaginous bacteria used as feedstock have numerous advantages, such as their high growth rate, being easy to cultivate, utilizing various substrates for growth, genetic or metabolic modifications possible. In addition, some species of bacteria are capable of carbon dioxide sequestration. Therefore, oleaginous bacteria can be a significant resource for the upcoming generation’s biodiesel production. This review discusses the biochemistry of lipid accumulation, screening techniques, and lipid accumulation factors of oleaginous bacteria, in addition to the overall general biodiesel production process. This review also highlights the biotechnological approach for oleaginous bacteria strain improvement that can be future used for biodiesel production and the advantages of using general biodiesel in place of conventional fuel, along with the discussion about global policies and the prospect that promotes biodiesel production from oleaginous bacteria.
Graphical Abstract
A new analytical approach for identifying market contagion
Authors: Lee Hee Soo , Kim Tae Yoon 
Published: April 2022

AbstractThis study proposed a new analytical approach to identify the excessive comovement of two markets as contagion. This goal is achieved by linking latent-factor and single-equation error correction models and evaluating the breaks in the short- and long-term relationships and correlatedness in the linked model. The results demonstrated that a short-term relationship representing the market speed ratio between two markets plays a key role in contagion dynamics. When a long-term relationship or correlatedness is broken (comovement change) due to a break in the short-term relationship (market speed ratio), contagion is highly likely and should be formally declared. Bayesian posterior probabilities were calculated to determine the cause. Furthermore, this study applied this analytical Bayesian approach to empirically test the contagion effects of the U.S. stock market during the global financial crisis between 2007 and 2009 using 22 developed equity markets.
Waiting reasons affecting the handling process at liquid bulk terminals
Authors: Salihoglu Eren , Bal Beşikçi Elif 
Published: April 2022

AbstractSince liquid bulk cargoes are the most frequently handled cargo types in Turkish ports in 2019 and the latter in 2020, the loading and unloading times of liquid bulk cargoes are important in terms of handling efficiency. While a higher amount of cargo handled per unit time increases the profitability for terminal operations, the short handling time of tankers at the terminal provides an advantage for the next voyage. In this study, the process in the terminals in Turkey where 19.05% of the total liquid bulk cargo handled was reviewed as well as the reasons for the waits in this process. Thus, it was aimed to find subjects that need to be focused on to reduce the waiting times. An expert team was asked to indicate the reasons for the waits using the fishbone method. In addition, a questionnaire was applied to a sample group of 134 people who were the stakeholders of the handling process. It was found out that the reasons for the waits obtained through the questionnaire matched those determined by the fishbone method. To reach a solution, prioritization was provided by scoring the reasons that lead to waits.
Entomopathogenic nematodes as potential and effective biocontrol agents against cutworms, Agrotis spp.: present and future scenario
Authors: Kumar Deepak , Kumari Pritam , Kamboj Rubal , Kumar Anil , Banakar Prakash , Kumar Vinod 
Published: April 2022

Abstract
Background
Cutworms (Agrotis spp.) are cosmopolitan and polyphagous pests distributed throughout world, which belong to the family Noctuidae, and about 26 species are found associated with agriculturally important crops in India and some other countries of world. The most prominent species belonging to genera Agrotis are Agrotis ipsilon, A. flammatra, A. plecta, A. spinifera and A. segetum. Cutworms cause substantial damage to many agricultural and horticultural crops particularly, at the seedling stage. This pest produces vitiating symptoms with a young stage (larvae) by feeding on the epidermis of leaves and eating away parts of the stem, tubers, etc.

Results
This review article is mainly focused on management of cutworm, which is very challenging due to larval hiding behaviour during the day time and feed actively at night. Efficient chemical control of cutworm may be obtained by adequately applying chemicals when young caterpillars are still on the leaves and therefore vulnerable. As per biology of cutworms, these pests remain hidden in cracks and crevices during most of life cycle so chemical control is often ineffective and economic. Sometimes, inadequate application of these chemicals is resulted into the development of resistance in these pests. Moreover, the adverse effects of the chemicals have led researchers to search for new control strategies. Recently, biological control has become a practical option for eco-friendly management of numerous insect pests. Among biological control, entomopathogenic nematodes (EPNs) have broad potential to kill the cutworms in soil itself.

Conclusions
Various species of EPNs like Steinernema spp. and Heterorhabditis spp. are found a quite effective and hold considerable potential to manage cutworms. So, the use of EPNs for the management of cutworms is a good alternate to chemical method.
Dynamical Casimir effects with atoms: From the emission of photon pairs to geometric phases
Authors: Impens François , de Melo e Souza Reinaldo , Matos Guilherme C. , Maia Neto Paulo A. 
Published: April 2022

Abstract
The coupling between a moving ground-state atom and the quantum electromagnetic field is at the origin of several intriguing phenomena ranging from the dynamical Casimir emission of photons to Sagnac-like geometric phase shifts in atom interferometers. Recent progress in this emerging field reveals unprecedented connections between non-trivial aspects of modern physics such as electrodynamic retardation, non-unitary evolution in open quantum systems, geometric phases, non-locality and inertia.