bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-125 family improves the radiosensitivity of head and neck squamous cell carcinoma
Authors: Wang Qi-Wei , Liu Jiang-Tao , Tan Li-Jun , Zhao Jian-Nan , Zhou Xiao-Jie , Yu Tian-Jiao , Sun Ya-Nan 
Published: June 2022

Abstract
Background
MiRNAs can affect the radiosensitization of head and neck squamous cell carcinoma (HNSCC). We aimed to analyze the function of miR-125 family members in HNSCC using The Cancer Genome Atlas (TCGA) and determine their effect on radiation in laryngeal squamous cell cancer (LSCC).
Methods
First, we performed survival analysis for patients with laryngeal cancer, conducted pathway enrichment analysis, and predicted target genes. Then, we performed transfection, cell proliferation assays, reverse transcription polymerase chain reaction, apoptosis assays, micronucleus tests, and western blotting on hep-2 cells selected with puromycin.
Results
MiR-125 family members exhibited significantly different expression in HNSCC. MiR-125b-1-3p, miR-125b-2-5p, and miR-125b-5p expression was significantly associated with tumor–node–metastasis staging, clinical cancer stages, and early histological grades in HNSCC. Radiation therapy had a statistically significant effect on the expression of all miR-125 family members, except miR-125a-3p. Moreover, miR-125a-5p was related to overall survival in LSCC. Thus, we predicted 110 target genes and seven hub genes of miR-125a-5p. MiR-125a-5p expression was significantly improved after transfection, and the proliferation rate of cells transfected with lentivirus vector expressing miR-125a-5p was significantly reduced compared to that of parental and negative control group cells. The radiation effect was also enhanced in cells transfected with miR-125a-5p. The ratio of apoptotic cells transfected and exposed to X-rays (10 Gy) was distinctly higher than that of the Ad-control group. Micronucleus assay results in the Ad-miR-125a-5p group were significantly different from those in the parental and Ad-control groups. Western blotting analysis revealed that miR-125a-5p upregulated the apoptotic regulators P53 and rH2AX. Thus, miR-125a-5p may increase radiosensitivity in LSCC via upregulation of pro-apoptotic genes.
Conclusions
MiR-125 family members could be prognostic biomarkers of HNSCC, and miR-125a-5p may substantially improve HNSCC sensitivity to radiotherapy by activating P53. Upregulating miR-125a-5p via lentivirus vectors may be a novel strategy to strengthen the effect of radiotherapy on laryngeal cancer.
The Prognostic Role of Right Ventricular Dysfunction in Patients with Hypertrophic Cardiomyopathy
Authors: Mushtaq Saima , Monti Lorenzo , Rossi Alexia , Pontone Gianluca , Conte Edoardo , Nicoli Flavia , Odoardo Luca Di , Guglielmo Marco , Indolfi Eleonora , Bombace Sara , Baggiano Andrea , Gripari Paola , Pepi Mauro , Bartorelli Antonio , Oliveira Margarida , Santos Ana , Francone Marco , Andreini Daniele 
Published: June 2022

Abstract
Background. Hypertrophic cardiomyopathy (HCM) primarily affects the left ventricle (LV) sparing the right ventricle (RV) in vast majority of cases. However, several studies employing CMR have revealed that myocardial hypertrophy may also involve the RV.Aim of the study. To assess RV size and function in a large prospectively cohort of HCM patients and to evaluate whether these parameters in association with other MR findings can predict cardiac eventsMethods. Two participating centers prospectively included patients with known or suspected HCM between 2011 and 2017. CMR studies were performed with three different scanners. Outcome measures were a composite of ventricular arrhythmias, hospitalization for HF and cardiac death.Results. Of 607 consecutive patients with known or suspected HCM, 315 had complete follow-up information (mean 65 ± 20 months). Among them, 115 patients developed major cardiac events (MACE) during follow-up. At CMR evaluation, patients with events had higher left atrium (LA) diameter (41.5 ± 8 mm vs. 37.17 ± 7.6 mm, p < 0.0001), LV mass (156.7 vs. 144 g, p = 0.005) and myocardial LGE (4.3% vs. 1.9%, p = 0.001). Similarly, patients with events had lower RV stroke volume index (42.7 vs. 47.0, p = 0.0003) and higher prevalence of both RV hypertrophy (16.4% vs. 4.7%, p = 0.0005) and reduced RV ejection fraction (12.2% vs. 4.4%, p = 0.006). In the multivariate analysis, LA diameter and RV stroke volume index were the strongest predictors of events (p < 0.001 and p = 0.0006, respectively)Conclusions Anatomic and functional RV anomalies detected and characterized with CMR may have may have a major role in predicting the prognosis of HCM patients.
Taiyuanostachya: An Abominable Angiosperm from the Early Permian of China
Authors: Wang Xin , Fu Qiang 
Published: June 2022

Abstract
Although angiosperms are can be clearly and strictly defined by their enclosed ovules, how old angiosperms are remains elusive. To solve this problem, the only reliable way is digging fossils. The currently widely accepted age for angiosperms is the Early Cretaceous, although this conclusion is facing increasing challenges pre-Cretaceous fossil evidence of angiosperms as well as molecular clocks. Here we report the outcome of our investigation on a Palaeozoic fossil plant, Taiyuanostachya gen. nov. Unlike previously thought, the fossil plant has both enclosed ovules and enclosed seeds. Since enclosed seeds are a feature characteristic of angiosperms, and enclosed ovules are idiosyncratic of angiosperms, the occurrence of both these features in Taiyuanostachya indicates that angiosperms, the single most diversified plant group on the Earth, may well have occurred in the Palaeozoic, and the origination of angiosperm appears to be much more complicated than assumed previously. Such a conclusion is astonishing for many but favors some molecular dating done decades ago.
High fat diet reveals sex-specific fecal and liver metabolic alterations in C57BL/6J obese mice
Authors: Wang Bo , Jadhav Vidya , Odelade Anuoluwapo , Chang Evelyn , Chang Alex , Harrison Scott H. , Maldonado-Devincci Antoinette , Graves Joseph , Han Jian 
Published: June 2022

Abstract
Obesity is a major health concern that poses significant risks for many other diseases, including diabetes, cardiovascular disease, and cancer. Prevalence of these diseases varies by biological sex. This study utilizes a mouse (C57BL/6J) model of obesity to analyze liver and fecal metabolic profiles at various time points of dietary exposure: 5, 9, and 12 months in control or high fat diet (HFD)-exposed mice. Our study discovered that the female HFD group has a more discernable perturbation and set of significant changes in metabolic profiles than the male HFD group. In the female mice, HFD fecal metabolites including pyruvate, aspartate, and glutamate were lower than control diet-exposed mice after both 9th and 12th month exposure time points, while lactate and alanine were significantly downregulated only at the 12th month. Perturbations of liver metabolic profiles were observed in both male and female HFD groups, compared to controls at the 12th month. Overall, the female HFD group showed higher lactate and glutathione levels compared to controls, while the male HFD group showed higher levels of glutamine and taurine compared to controls. These metabolite-based findings in both fecal and liver samples for a diet-induced effect of obesity may help guide future pioneering discoveries relating to the analysis and prevention of obesity in people, especially for females.
Hsa_circ_0000670 regulates cell growth, angiogenesis and glutamine metabolism of gastric cancer cells through miR-141-3p/ALKBH1 axis
Authors: Lu Jiajun , Zhou Yuan , Chen Zhiheng , Jiang Honggang , Li Jin , Dou Guangjian 
Published: June 2022

Abstract
Background: Gastric cancer (GC) is one of the four most deadly tumors in the world, and its occurrence is influenced by genetic and environmental factors. CircRNA-miRNA-mRNA is reported to be associated with variety cancer, including GC. Methods: The expression of hsa_circ_0000670, miR-141-3p and AlkB homolog 1 (ALKBH1) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation and apoptosis were detected by 5-Ethynyl-2’-deoxyuridine (EdU) assay and flow cytometry. The protein expression levels were measured by western blot and immunohistochemistry (IHC). Tube forming experiment was performed to assess angiogenesis rate. The levels of glutamine and α-ketoglutaric acid (α-KG) were examined using commercial kits. The target interaction between miR-141-3p and circ_0000670 or ALKBH1 was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. The effect of circ_0000670 on tumor in vivo was analyzed in mice.Results: Circ_0000670 had a stable ring structure and was accelerated in GC tissues and cells. The proliferation of GC was hampered by circ_0000670 knockdown in vivo and in vitro, and the apoptosis rate was increased by circ_0000670 knockdown. Circ_0000670 down-regulation suppressed angiogenesis, the level of glutamine and α-KG in GC cells. The impacts of circ_0000670 knockdown on GC were relieved by miR-141-3p inhibition. The results indicated that circ_0000670 silencing could induce cell proliferation decline in GC, which was ameliorated by miR-141-3p knockdown. The functions of miR-141-3p mimic on cell proliferation, apoptosis, angiogenesis, glutamine and α-KG were reversed by ALKBH1 up-regulation.Conclusion: Our findings revealed that circ_0000670 promoted GC progression though miR-141-3p and ALKBH1, suggesting that circ_0000670 might be a target for GC clinical treatment.
Correlation between Eye Movements and Asthenopia: A Prospective Observational Study
Authors: Liu Ziyuan , Zhang Kaiyun , Gao Shuang , Yang Jiarui , Qiu Weiqiang 
Published: June 2022

Abstract
Purpose: To analyze the correlation between eye movements and asthenopia so as to explore the possibility of using eye-tracking techniques for objective assessment of asthenopia.Methods: This prospective observational study used the computer visual syndrome questionnaire to assess the severity of asthenopia in 93 enrolled college students who complained about asthenopia. Binocular accommodation and eye movements during the reading task were also examined. The correlations between the questionnaire score and accommodation examination results and eye movements parameters were analyzed. Differences in eye movement parameters between the first and last reading paragraphs were compared. The trends in eye movement changes over time were observed. Results: About 81.7% subjects suffered from computer visual syndrome.The computer visual syndrome questionnaire total score was positively correlated with positive relative accommodation (P<.05). In the first reading paragraph, a double vision was positively correlated with the unknown saccades (all P<.05). Difficulty focusing at close range was positively correlated with total fixation duration, total visit duration, and reading speed (all P<.05). Feeling that sight was worsening was positively correlated with the regressive saccades (P<.05). However, visual impairment symptoms were not significantly correlated with any accommodative function. In a total of 20-minute reading, significantly reduced eye movement parameters were: total fixation duration, fixation count, total visit duration, visit count, fixation duration mean, and reading speed (all P<.01). The eye movement parameters that were significantly increased were: visit duration mean and unknown saccades (all P<.001).Conclusion: Eye tracking could be used as an effective assessment for asthenopia.
Erythritol alters gene transcriptome signatures, cell growth, and biofilm formation in Staphylococcus pseudintermedius
Authors: Fujii Tadashi , Tochio Takumi , Nishifuji Koji 
Published: June 2022

Abstract
· Background: Erythritol has recently been shown to have an inhibitory effect on the growth of Staphylococcus pseudintermedius isolated from dogs with superficial pyoderma. The present study aimed to define the changes in gene transcription signatures induced by erythritol in S. pseudintermedius. Changes in the gene transcription profiles of S. pseudintermedius by the presence of erythritol were analyzed by RNA sequencing and quantitative PCR. Gene ontology analysis was performed to assign functional descriptions to the genes.· Results: We revealed that erythritol induced up-regulation of three genes (ptsG, ppdK, and ppdkR) that are related to the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). Glucose supplementation restored the up-regulation of the PTS-related genes in response to erythritol. In addition, erythritol down-regulated eleven genes that are located in a single pur-operon and inhibited biofilm formation.· Conclusions: These findings indicated that erythritol antagonistically inhibits PTS-mediated glucose uptake, thereby exerting a growth inhibitory effect on S. pseudintermedius. Moreover, erythritol inhibits the 'de novo' IMP biosynthetic pathway that may contribute to biofilm synthesis in S. pseudintermedius.
Effects and optimal dose of exercise on endothelial function in patients with heart failure: a systematic review and meta-analysis
Authors: Fuertes-Kenneally Laura , Manresa-Rocamora Agustín , Blasco-Peris Carles , Ribeiro Fernando , Sempere-Ruiz Noemí , Sarabia José Manuel , Climent-Paya Vicente 
Published: June 2022

Abstract
Background
Exercise-based cardiac rehabilitation (CR) is considered an effective treatment for enhancing endothelial function in patients with heart failure (HF). Nonetheless, recent studies have been published and the optimal “dose” of exercise required to increase the benefits of exercise-based CR programmes on endothelial function is still not well-known.
Objectives
(a) To estimate the effect of exercise-based CR on endothelial function, assessed by flow-mediated dilation (FMD); (b) to determine whether high-intensity interval training (HIIT) is better than moderate intensity training (MIT) for improving FMD; and (c) to investigate whether there are differences between resistance exercise (alone or combined with aerobic exercise) and aerobic exercise for enhancing endothelial function.
Methods
Electronic searchers were carried out in PubMed, Embase, and Scopus up to February 2022. Random-effects models of between-group mean differences were estimated. Heterogeneity analyses were performed by means of the chi-square test and I2 index. Subgroup analyses and meta-regressions were used to test the influence of potential moderator variables on the effect of exercise.
Results
Most of the included studies performed an aerobic-based CR programme in patients with HF and reduced ejection fraction (HFrEF). We found a FMD increase of 2.74% (95% confidence interval [CI] = 1.69, 3.79%) in favour of CR programmes compared with control groups. Nonetheless, the results of included studies were inconsistent (p < .001; I2 = 94.8%). Higher FMD improvement was found in studies performed with patients with HFrEF compared to patients with HF and preserved ejection fraction, reported radial FMD, or performed higher training frequency. Moreover, HIIT enhanced FMD to a greater extent than MIT (2.65% [95% CI = 0.98, 4.33%]), while no differences were found based on the exercise modality (1.97% [95% CI = − 0.64, 4.58%]).
Conclusion
Aerobic-based CR is a non-pharmacological treatment for enhancing endothelial function in patients with HFrEF. Nonetheless, training variables such as the aerobic training method (i.e., HIIT and MIT) and training frequency should be considered to properly design exercise-based CR programmes.
Registration:
The protocol was prospectively registered on the PROSPERO database (CRD42022304687).
Utilization of family planning services in Africa's most populous nation, are we at a tipping point
Authors: Orjingene Obinna , Teryila Ogojah 
Published: June 2022

Abstract
Background
Nigeria is the most populous country in Africa, with a population growth rate of 2.53 percent, one of the highest contributors to the global maternal and newborn deaths burden. Family planning service utilization has remained low despite efforts by development partners and the government. Hence, the need to examine the utilization of family planning services in order to understand regional preferences, trends, patterns, and utilization associated factors such as accessibility and availability of the services to the populace.
Methodology
Critical analysis regarding the utilization of family planning in Nigeria was used with source data from NDHS 2003, 2008, 2013, and 2018 and DHIS2. The analysis focused on the utilization of family planning services and most utilized family planning options.
Finding
Contraceptive use among women of reproductive age is generally higher in the northern region than in the southern region. A five-year trend in the utilization of family planning services by women of reproductive age is showed a slightly declining trend in the utilization of family planning services by women of reproductive age in the country. Injectables and implants were married women's most utilized family planning options. As reported by health facilities, utilization of family planning services in 2021 showed that condoms and implants were the most utilized family planning options.
Conclusion
The findings urge the need to intensify sensitization among the rural populace on the benefits of family planning, improve female literacy and empowerment, and make easily accessible family planning services and commodities.
Patient-Reported Outcomes in RA patients treated with tofacitinib or bDMARDs in real-life conditions in two Latin American countries
Authors: Manuel Reyes Juan , Gutierrez Magda , Madariaga Hugo , Escalante Willian Otero , Guzman Renato , Izquierdo Jorge , Abello Mauricio , Sanchez Patricia Velez , de Leon Dario Ponce , Lukic Tatjana , Amador Luisa 
Published: June 2022

Abstract

Objective
To describe the efficacy, safety and patient-reported outcomes (PROs) in patients with rheumatoid arthritis (RA) with an inadequate response to conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) treated with tofacitinib or biological DMARDs (bDMARDs) in real-life conditions.
Methods
A noninterventional study was performed between March 2017 and September 2019 at 13 sites in Colombia and Peru. Outcomes measured at baseline and at the 6-month follow-up were disease activity (RAPID3 [Routine Assessment of Patients Index Data] score), functional status (HAQ-DI [Health Assessment Questionnaire] score), and quality of life (EQ-5D-3L [EuroQol Questionnaire]). The Disease Activity Score-28 (DAS28-ESR) and frequency of adverse events (AEs) were also reported. Unadjusted and adjusted differences from baseline were estimated and expressed as the least squares mean difference (LMDs).
Results
Data from 100 patients treated with tofacitinib and 70 patients with bDMARDs were collected. At baseline, the patients’ mean age was 53.53 years (SD 13.77), the mean disease duration was 6.31 years (SD 7.01). The change from baseline at month 6 was not statistically significant different in the adjusted LMD [SE] for tofacitinib vs. bDMARDs for RAPID3 score (-2.55[0.30] vs. -2.52[0.26]), HAQ-DI score (-0.56[0.07] vs. -0.50[0.08]), EQ-5D-3L score (0.39[0.04] vs. 0.37[0.04]) and DAS28-ESR (-2.37[0.22] vs. -2.77[0.20]). Patients from both groups presented similar proportions of nonserious and serious AEs. No deaths were reported.
Conclusion
Changes from baseline were not statistically significant different between tofacitinib and bDMARDs in terms of RAPID3 scores and the secondary outcomes. Patients from both groups presented similar proportions of nonserious and serious AEs.
Clinical trial number:
NCT03073109
Multi Driven EDT Clustering for Wireless Chemical Sensor Network
Authors: John Abin , R Asaletha 
Published: June 2022

Abstract
WCSN is one of the most important research areas in the terrestrial networking field due to its wide range of applications. One of the most difficult challenges is expanding the overall running time without attaching any new batteries or hardware. Using a novel method called EDT (Energy, Distance, Time) driven strategy, this paper proposes a clustering algorithm to solve the problems of the hotspot as well as reduce battery energy loss. The CH rotation method was then described in detail. In this paper, will introduce a new function called SCH (Substitute cluster head), which has replaced CH. The main objectives of this research are to improve energy reliability and network lifetime. Finally, the presented EDT approach can be comparable to current algorithms, where LEACH's network lifetime is 15.4 %, DECSA's is 23.2 %, and NEAP's is 11.4 %, but our proposed EDT methodology extends network lifetime by 40% as well as decreases energy usage by 7% as compared to LEACH when determining the SCH.
A Hybrid Intrusion Detection System against Botnet Attack in IoT using Light Weight Signature and Ensemble Learning Technique
Authors: Babu Erukala Suresh , Srinivasa Mekala , Pemula Rambabu , Nayak Soumya Ranjan , Shankar Achyut 
Published: June 2022

Abstract
Internet of Things (IoT) plays a substantial role in the digital era of the information and intelligent Age. The use of interactive internet apps has opened up opportunities for increased threats to cyber security. Recently, botnets threats in IoT had become the most common cyber security threats. These threats provide malicious services and carry out phishing links on the internet. Consequently, an efficient intrusion detection system (IDS) is needed to detect these botnet attacks and unknown attacks with a low false-positive rate. Existing IDS methods detect these new attacks but require a high-precision detector. Most of the existing IDS uses either single machine learning or multiple classifiers that fail to detect unknown attacks and produce a high false-positive rate. This paper proposes a hybrid-based IDS that solves and detects unknown and novel attacks with low false-positive rates, better accuracy levels, and detection rates. This proposed work is deployed using two IDS methods in a two-staged manner. First, we modelled a Signature-Based Detector against DDOS attack for providing a better detection rate, early detection of known and low false-positive rates. Next, we modelled an Anomaly-Based Detector against DDOS attacks to achieve low false alarm rates, improved accuracy levels, and detected botnet and unknown attacks using the Machine Learning-based ensemble technique. Finally, we evaluated the performance using the confusion matrix on the classified data. We assessed the classifier performance based on detection rate, precision, accuracy, AUC score, and false-positive rates. The proposed hybrid technique provides a lower false-positive rate and better detection rate than the proposed model's classification technique.