bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bovine campylobacteriosis in bulls: insights in the conventional and molecular diagnosis
Authors: García Juan A. , Farace Pablo , Gioffré Andrea K. , Morsella Claudia , Méndez María A. , Acuña Joaquín , Aller Juan F. , Signorini Marcelo , Paolicchi Fernando A. 
Published: May 2022

Abstract
Campylobacter fetus is a gram-negative motile bacterium, with two subspecies relevant for cattle health: C. fetus subsp. venerealis (Cfv) and C. fetus subsp. fetus (Cff). Both subspecies are associated with reproductive losses in cattle. Herein, the C. fetus infection of three Angus bulls naturally challenged with Cfv, Cfv biovar intermedius (Cfvi) or Cff were assessed through conventional and molecular diagnosis of preputial smegma (PS) samples collected with different devices. The Cfv- and Cfvi-bulls were positive until at least 9 months. Although Cff is not considered a venereal strain, this study demonstrated its transmission to bull from heifers experimentally infected, with evidence of colonization and persistence in the preputial cavity for 5 to 6 months. This finding suggests a potential risk of dissemination of the bacteria within herds. The results obtained by bacteriological culture or direct immunofluorescence (DIF) showed no significant differences, regardless the sampling device used (aspiration with Cassou pipette, metal and plastic scraper). On the other hand, the qPCR for C. fetus detection yielded better results with an in-house DNA extraction method than with a commercial kit (75% vs 66.6%). Furthermore, the molecular diagnosis by qPCR was more efficient than culture (66.6%) or DIF (56%). Bacteremia in whole blood samples was negative by qPCR and bacteriological culture in all samples. PCR-based methods are promising for the diagnosis of Bovine Genital Campylobacteriosis from clinical samples of PS. This study demonstrated the transmission of Cff from heifers to bull.
Microbial Quality and Antimicrobial Residue of Local and Industrial Processed Fruit Juice Sold in Tamale, Ghana
Authors: Jimma Fidaus Iddrisu , Mohammed Amina , Adzaworlu Emmanuel Gameli , Nzeh Joseph , Quansah Lydia , Dufailu Osman Adamu 
Published: May 2022

Abstract
Fruits are essential part of human nutrition that provides numerous health benefits. When processed into juice, they are either packaged and stored or consumed immediately. There are reports on contraction of foodborne illness due to consumption of these natural beverages possibly from contamination by pathogenic microbes. Also, concerns of antimicrobial resistance due to antimicrobial residues in fruit juices has been raised. Thus, this study assessed the microbial quality of fruit juice by determining the microbial load of E. coli and Salmonella spp., and the presence of antimicrobial residues in locally processed fruit juices (n= 25) and industrially (n= 3) processed fruit juices sold in Tamale, Ghana. Spread plate technique was adopted for isolation and enumeration of bacteria. Whereas the presence of antimicrobial residue was determined using the Premi® test kit. Neither E. coli nor Salmonella spp. is recorded in all three industrially processed juice samples. However, the locally processed juice samples recorded 88 % E. coli and 40 % Salmonella spp. with microbial load above the acceptable limits. Antimicrobial residues were absent in all 28 samples analyzed. The incidence of high microbial load found in the locally processed fruit juice is of concern to avert possible foodborne illness linked with the consumption of fruit juices within Tamale.
Structural stigma and its impact on healthcare for borderline personality disorder: a scoping review
Authors: Klein Pauline , Fairweather A. Kate , Lawn Sharon 
Published: May 2022

Abstract
Background
People with Borderline Personality Disorder (BPD) and their carers/families continue to experience structural stigma when accessing health services. Structural stigma involves societal-level conditions, cultural norms, and organizational policies that inhibit the opportunities, resources, and wellbeing of people living with attributes that are the object of stigma. BPD is a serious mental illness characterized by pervasive psychosocial dysfunction including, problems regulating emotions and suicidality. This scoping review aimed to identify, map, and explore the international literature on structural stigma associated with BPD and its impact on healthcare for consumers with BPD, their carers/families, and health practitioners.
Methods
A comprehensive search of the literature encompassed MEDLINE, CINAHL, PsycINFO, Scopus, Cochrane Library, and JBI Evidence-Based databases (from inception to February 28th 2022). The search strategy also included grey literature searches and handsearching the references of included studies. Eligibility criteria included citations relevant to structural stigma associated with BPD and health and crisis care services. Quality appraisal of included citations were completed using the Mixed Methods Appraisal Tool 2018 version (MMAT v.18), the Joanna Briggs Institute (JBI) Checklist for Systematic Reviews and Research Syntheses Tool, or the AGREE II: advancing guideline development, reporting, and evaluation in health care tool. Thematic Analysis was used to inform data extraction, analysis, interpretation, and synthesis of the data.
Results
A total of fifty-seven citations were included in the review comprising empirical peer-reviewed articles (n = 55), and reports (n = 2). Studies included quantitative, qualitative, mixed methods, and systematic review designs. Review findings identified several extant macro- and micro-level structural mechanisms, challenges, and barriers contributing to BPD-related stigma in healthcare systems. These structural factors have a substantial impact on health service access and care for BPD. Key themes that emerged from the data comprised: structural stigma and the BPD diagnosis; structural stigma surrounding health and crisis care services; and practitioner-patient interactions.
Conclusion
Narrative synthesis of the findings provide evidence about the impact of structural stigma on healthcare for BPD. Results of this review are anticipated to inform future research, policy, and practice in addressing BPD-related stigma in healthcare systems, as well as approaches for improving responsive care delivery for consumers with BPD and their carers/families.
Capturing Critical Data Elements in Juvenile Idiopathic Arthritis: Variations between In-person and Virtual Visits and Initiatives to Improve Data Capture
Authors: Ryan Meghan E. , Warmin Andrew , Binstadt Bryce A. , Correll Colleen K. , Hause Emily , Hobday Patricia , Lerman Alison , Mahmud Shawn A. , Riskalla Mona M. , Shaheen Zachary , Vehe Richard K. , Bullock Danielle R. 
Published: May 2022

Abstract
Background: Documentation of critical data elements is a focus of the Pediatric Rheumatology Care and Outcomes Improvement Network to aid in clinical care and research for patients with juvenile idiopathic arthritis. We aimed to increase data capture for arthritis pain score, patient/parent global assessment of wellbeing, provider global assessment, and active joint count. We hypothesized that data capture for all critical data elements would be lower for virtual visits compared to in-person visits. Methods: All visits for patients with JIA between 9/14/2020 and 12/31/2021 at the University of Minnesota were included. Sixteen interventions with providers were conducted, including email reminders, individual discussions, group meetings, and feedback reports. We used statistical process control charts to evaluate change over time.Results: Baseline included 355 patient-visits: 221 (62%) in-person and 134 (38%) virtual with critical data elements entry ranging between 50-60%. Post-intervention included 1,596 patient-visits: 1,350 (85%) in-person and 246 (15%) virtual with critical data elements entry of 91%. In-person visits had significantly higher data capture rates than virtual visits for all 4 critical data elements.Conclusion: We achieved our aim to increase critical data element documentation and found that collection of critical data elements occurred significantly less often with virtual visits than with in-person visits. We inferred that critical data element capture for virtual visits was lower for multiple reasons, including the lack of intake forms and provider uncertainty about scoring certain elements. Now that we improved capture of critical data elements, we can shift the focus to efforts aimed at improving outcomes for patients with juvenile arthritis.
Associations of left renal vein entrapment with IgA nephropathy and glomerular galactose-deficient-IgA1 deposition
Authors: Wang Fengmei , Zhu Huizi , Bao Shougang , Qi Hengtao , Xu Liang , Liu Xiang , Zhai Chunjuan , Wang Rong , Yang Xiaowei 
Published: May 2022

Abstract
Objectives: To identify whether left renal vein (LRV) entrapment was more prevalent in IgA nephropathy (IgAN) and Henoch-Schönlein purpura (HSPN) compared with other types of renal diseases, and the association of LRV entrapment with glomerular incidental IgA and galactose-deficient-IgA1 (Gd-IgA1) deposition.Methods: 797 patients with biopsy-proven kidney diseases have been screened for LRV entrapment by color Doppler ultrasound, and the prevalence of LRV entrapment in different types of renal diseases were then analyzed. Propensity score matching analysis was used to adjust for age, gender and body mass index. Immunostaining of Gd-IgA1 with KM55 was performed in paraffin-embedded sections of renal biopsy specimens.Results: LRV entrapment was diagnosed in 47 patients (6%) with several kinds of renal diseases in our cohort. 32 (68%) LRV entrapments were combined with expanded IgAN (idiopathic IgAN and HSPN). The prevalence of LRV entrapment in expanded IgAN was significantly higher than that in non-expanded IgAN (17% vs. 2%, P<0.001), even after adjustment for age, gender and body mass index by propensity score matching analysis (13% vs. 2%, P<0.001). Removing expanded IgAN and LN, glomerular incidental IgA deposition was observed to be significantly more common in patients with LRV entrapment compared with patients without it (43% vs. 9%, P<0.001). Furthermore, in glomerular diseases with incidental IgA deposits, significantly much larger proportion of patients with LRV entrapment were positive for glomerular Gd-IgA1 in contrast to patients without LRV entrapment (5/5 vs. 5/17, P=0.01).Conclusion: LRV entrapment might be associated with glomerular Gd-IgA1 deposition and the development of IgAN.
Effective Arterial Elastance as The Best Predictor of Mean Arterial Pressure in Patients with Sepsis or Septic Shock Receiving Fluid Expansion: A Validation Study by Regression Analysis
Authors: Theerawit Pongdhep , Tengsirikomol Tanawat , Sutherasan Yuda 
Published: May 2022

Abstract
Backgroundwe conducted this study to compare four arterial load parameters and determine which arterial load parameters directly impacted arterial pressure regarding pressure, flow, and arterial system relationship.MethodsWe conducted a cross-sectional study in patients with sepsis who underwent volume expansion (VE). Hemodynamic parameters were recorded before and after VE. The relationship between the change of mean arterial pressure (%MAP) and that of the dynamic arterial elastance (EaDyn), effective arterial elastance (Eaeff), net arterial elastance (EaNet), and net arterial resistance (RaNet) was analyzed.ResultsSixty-two patients were included. The DEaDyn(%) was not correlated with DMAP(%) (r=0.048, P=0.826). Meanwhile, DEaeff(%), DEaNet(%), and DRaNet(%) were correlated with DMAP(%) (r=0.495, P<0.001; r=0.453, P<0.001; and r=0.485, P<0.001, respectively). A multiple linear regression model was analyzed for identifying predictors of DMAP(%) by including DCO(%) and each %change of arterial parameters. The best-fit model was found by including DCO(%) and DEaeff(%) in the regression equation (R2=0.823, adjusted R2=0.817). The model was adjusted by age, sex, Acute Physiology and Chronic Health Evaluation II (APACHE II) score, Sequential Organ Failure Assessment (SOFA) score, arterial lactate level, norepinephrine dosage, ventilator setting, method of VE, and fluid responsiveness and found that DCO(%) and DEaeff(%) remained statistically significant predictors of DMAP(%) (P<0.001 and P<0.001, respectively).ConclusionThe Eaeff was the best AL parameter that correlated with the changes in MAP. Furthermore, the model that included the ∆Eaeff(%) provided the best predictive performance for ∆MAP(%) induced by VE, independent of the fluid responsiveness and norepinephrine dosage.
Delay in Diagnosis of Common Cancers among Women – A Scoping review
Authors: R Maanasa , S Jayashree , Srijaya Parameswari , PR Karthikeyan , Govindarajulu Srinivas 
Published: May 2022

Abstract
Introduction
As majority of these cancers are only diagnosed at advanced stages, it has become the reason for increased mortality rates and poor prognosis affecting the quality of life of patients. The delay in cancer diagnosis especially among women may be attributed to various factors both from patients and the healthcare sector. This study thus aims to conduct a comprehensive review of the literature on the factors behind the delay in case detection, that are common and specific for both the cancers, the reasons for the delay and the average time duration and reasons for the delay.
Materials and methods
A systematic search was conducted over several databases to identify relevant peer-reviewed studies. From eligible studies, data were extracted to summarize, collate, appraise the quality and make a narrative account of the findings.
Results
Of 581 studies, 29 were reviewed which revealed the commonest factors for delay were patient (presentation delay) followed by the health provider and healthcare sector, and the average time duration of delay was 6 months. Reasons for delay in the presentation were misconception about the disease, perceived stigma and fear of cancer. Poor knowledge of general practitioners, misclassification of disease severity, and long waiting queues in public health facilities were the commonest reasons for healthcare sector delay.
Conclusion:
Disease awareness among healthy women followed by widespread screening and appropriate interpretation of early clinical presentation by healthcare providers can be improved to reduce delay in cancer diagnosis and ensure better prognosis and quality of life of patients.
Research on the driving effect of Eco-economic Zones on urban coordinated development and its effect mechanism
Authors: Liu Jiaqi , Gong Bibang , Hu Zongyi , Li Jianjun 
Published: May 2022

Abstract
The core goal of Eco-economic Zone planning is to enhance the coordinated development of cities in the basin in terms of main function realization and livelihood development and achieve coordinated and high-quality development in the basin. Using a double-difference approach, this paper uses panel data from 2004 to 2019 for 49 prefecture-level cities in Jiangxi, Hunan, Hubei, and Jiangsu provinces to test the proposed theoretical hypotheses and empirically examine the driving effects and mechanisms of the three Eco-economic Zones on the coordinated development of cities along the river basin. The study finds that the three zones significantly enhance the coordinated development of cities along the zones. The dimension of livelihood development is more significant in strengthening cities' coordinated development along the three zones. At the same time, there is substantial regional individual heterogeneity and temporal dynamic heterogeneity in the driving effect of the plan. The Eco-economic Zone Plan is designed to enhance the coordinated development of cities along the Great Lakes Basin through green economic development and ecological environment improvement. The above findings help explore new experiences in the protection, management, and development of the Great Lakes Basin. They have important policy implications for cities in the Eco-economic Zone to develop a “new path” of collaborative ecological and economic development.
Development and validation of two redox-related genes associated with prognosis and immune microenvironment in endometrial carcinoma
Authors: He Yan , Cao Nannan , Tian Yanan , Wang Xuelin , Xiao Qiaohong , Tang Xiaojuan , Huang Jiaolong , Zhu Tingting , Hu Chunhui , Zhang Ying , Deng Jie , Yu Han , Duan Peng 
Published: May 2022

Abstract
Background: In recent studies, tumourigenesis and development of endometrial carcinoma (EC) have been correlated significantly with redox. We aimed to develop and validate a redox-related prognostic model of patients with EC to predict the prognosis and the efficacy of immunotherapy.Methods: We downloaded gene expression profiles and clinical information of patients with EC from the Cancer Genome Atlas (TCGA) and the Gene Ontology (GO) dataset. We identified two key differentially expressed redox genes (CYBA and SMPD3) by univariate Cox regression and utilised them to calculate the risk score of all samples. Based on median of risk scores, we composed low-and high-risk groups and performed correlation analysis with immune cell infiltration and immune checkpoints. Finally, we constructed a nomogram of the prognostic model based on clinical factors and the risk score. We verified the predictive performance by using receiver operating characteristic (ROC) and calibration curves.Results: CYBA and SMPD3 were significantly related to prognosis of patients with EC and used to construct a risk model. There were significant differences in survival, immune cell infiltration and immune checkpoints between the low-and high-risk groups. The nomogram developed with clinical indicators and the risk scores was effective in predicting the prognosis of patients with EC.Conclusion: Our novel prognostic model based on CYBA and SMPD3 accurately predicted the prognosis and immunotherapy outcomes of patients with EC. Our results provide a new direction to study tumourgenesis and development, as well as guidance for clinical diagnosis and therapy of EC.
Clinical teaching practices in maternal mental health nursing. An integrated review
Authors: Hussein Chrissie , Baluwa Masumbuko , Bvumbwe Thokozani 
Published: May 2022

Abstract
Background
Clinical teaching of nursing and midwifery is critical for acquiring necessary competencies and developing confidence in students to make them ready for practice. Such competencies are especially important in midwifery practice in Malawi as there is a significant increase in maternal mental health problems in the country. Most cases are reported in the primary healthcare level maternity units where qualified nurse-midwives practise. However, reports show that maternal mental health has not been given adequate consideration and there is a huge service gap among nursing and midwifery personnel globally and in Malawi. This integrative review examined the literature on clinical teaching practices in maternal mental health to inform nursing and midwifery educators on the competencies taught in nursing colleges to improve the quality, quantity, and relevance of nursing and midwifery education
Method
PubMed, MEDLINE, Research4life, HINARI, Google scholar, and EBSCOhost were searched using clinical teaching, clinical practices, perinatal mental health, maternal mental health, clinical skills, and clinical education as keywords. Published works from 2014 to 2022 were reviewed.
Results
Six themes emerged; core curriculum, regulation of professional standards, clinical teaching strategies, a collaboration between educators and staff, capacity building, and availability of resources.
Conclusion
Nursing and midwifery training institutions should review their curriculum to maximize student learning opportunities in maternal mental health practice by creating more clinical-based learning opportunities that meet the students’ needs and those of the public.
Skin aseptic abscess secondary to dermatomyositis for 8 years follow-up: A case report and literature review
Authors: Ma Ke , Liu Zhihe , Shao Shihong , Wen Dawei , Zhao Lei , Liu Jingxuan , Zhu Fangyi , Liang Hongda 
Published: May 2022

Abstract
Background Dermatomyositis occurs with aseptic purulent changes in the skin is relatively rare. T he purpose of this report is to explore the clinical characteristics, etiology, diagnosis a nd treatment of dermatomyositis combined with skin aseptic abscess.Case presentation We report a case of dermatomyositis and skin aseptic abscess. After combined treatm ent with hormones, cyclosporine and cyclophosphamide, the skin aseptic abscess was effectively controlled.Conclusion Dermatomyositis and skin aseptic abscess are rare, and active treatment of the primar y disease is an alternative treatment strategy.
The relationship of GDF-15 with renal damage and dyslipidemia in non-albuminuric and albuminuric Type-2 Diabetes Mellitus
Authors: Yücel Hasan Esat , İlanbey Bilal 
Published: May 2022

Abstract
Aim: To investigate the correlation of GDF-15 with renal damage and dyslipidemia in Type-2 Diabetes Mellitus.Material and Method: The study was conducted prospectively with patients diagnosed with Type-2 Diabetes Mellitus. Two groups were formed as non-albuminuric (n:47) and albuminuric (n:24). Age, gender, comorbidities, GDF-15, glycemic index, lipid panel, GFR, urine albumin/creatin (ACR) and urine protein/creatin (PCR) ratios of the groups were compared and their correlations were examined.Results: GDF-15, age and hemoglobin A1c were found to be higher in the albuminuric group, and hemoglobin and hematocrit levels were found to be lower. A positive correlation of GDF-15 with urine ACR/PCR was observed in the albuminuric group. In the non-albuminuric group, was observed positive correlation with Triglyceride and negative correlation with HDL. Negative correlation of GDF-15 and GFR was detected in all participants.Conclusions: In type-2 DM, GDF-15 is positively correlated albuminuria and TRG elevation and negatively correlated with GFR and HDL
Establishment of a lipid metabolism disorder model in ApoEb mutant zebrafish
Authors: Hu Yang-Xi , You Hong-Min , Zhu Rong-Fang , Liang Yu-Lai , Li Fang-Fang , Qin Yong-Wen , Zhao Xian-Xian , Liang Chun , Jing Qing 
Published: May 2022

Abstract
Background and aims: ApoEb
is a zebrafish homologous to mammalian ApoE, whose deficiency would lead to lipid metabolism disorders (LMDs) like atherosclerosis. We attempted to knock out the zebrafish ApoEb, then establish a zebrafish model with LMD.
Methods
ApoEb was knocked out using CRISPR/Cas9 system, and the accumulation of lipids were confirmed by Oil Red O staining, confocal imaging, and lipid measurements. The lipid-lowering effects of simvastatin (SIM), ezetimibe (EZE) and Xuezhikang (XZK), an extract derived from red yeast rice, were evaluated through in vivo imaging in zebrafish larvae.
Results
In ApoEb mutant, significant vascular lipid deposition occurred, and lipid measurement performed in whole-body homogenate of larvae and adult plasma showed significantly increased lipid levels. SIM, EZE and XZK apparently relieved the hyperlipidemia in ApoEb mutants, and XZK had a significant inhibitory effect on the recruitment of neutrophils and macrophages.
Conclusions
In this study, a LMD model has been established in ApoEb mutant zebrafish. We suggest that this versatile model could be applied in studying hypercholesterolemia and related vascular pathology in the context of early atherosclerosis as well as the physiological function of ApoE.