bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drill-and-Prechop Technique: Modification of the Drill-and-Crack Technique for Mature Cataracts
Authors: Xu Mao , Qi Yongjun , Weng Yongde , Yang Yang , Deng Jianhua , Liu Wanjun , Mo Ting , Ye Xiangxiang 
Published: July 2022

Abstract
Background:There are some techniques for disassembly of hard nuclear.It is challenging in hard cataract surgery through microincision.The classic chop or prechop techniques often do not succeed,resulting in incomplete nuclear segmentation.The authors describe a new chop technique for removing hard nucleus cataracts in coaxial microincisional cataract surgery.Methods:We have termed this new technique“drill and prechop”.We create a deep hole (drill) in the central nucleus with the phaco tip and divide the nucleus (prechop) with the Nagahara chopper and the modified capsulorhexis forceps inside the hole.The chopper and the modified capsulorhexis forceps are spread apart laterally after they approach at the center of the nucleus, to create a complete fracture across the entire nucleus.Since January 2022, we have completed 27 eyes of 25 patients with hard nucleus cataract using this technique. Results:Complete segmentation of the hard nuclear into two hemispheres was implemented with this drill and prechop technique in all cases.The phaco time and energy decreased significantly.No intraoperative complication such as iris injury, anterior capsule tears, zonulysis, or posterior capsule rupture with vitreous loss occurred during surgery.Conclusions:This technique simplifies the previous prechop techniques especially for hard nucleus in microincisional cataract surgery. The technique is efficient, safe and simple.Even inexperienced surgeons are able to quickly master this technique.
Chemical composition and bioactivity of oilseed cake extracts obtained by subcritical and modified subcritical water
Authors: Švarc-Gajic Jaroslava , Rodrigues Francisca , Moreira Manuela M. , Spigno Giorgia , Delerue-Matos Cristina , Morais Simone , Doresh Olena , Silva Ana Margarida , Bassani Andrea , Dzedik Valentin 
Published: July 2022

Abstract
Recovery of bioactive compounds from biowaste is gaining more and more interest in circular economy models. The oilseed cakes are usually insufficiently exploited by most technologies since they represent valuable matrices abundant in proteins, minerals, and phytochemicals, but their use is mostly limited to feed ingredients, fertilizers or biofuel production. This study was thus focused on the exploration of new valorization pathways of oilseed cakes by subcritical water, representing a safe and economic alternative in the creation of value chains. Pumpkin, hemp, and flax seed cakes were treated with subcritical water in nitrogen and carbon-dioxide atmospheres, as well as in nitrogen atmosphere with the addition of acid catalyst. The degradation of carbohydrate fraction was studied by quantifying sugars and sugar degradation products in the obtained extracts. The extracts obtained under different conditions were further compared chemically with respect to total phenols and flavonoids, as well as to the content of individual phenolic compounds. Furthermore, the effects of subcritical water treatment conditions on antioxidant, antiradical and cytotoxic properties of thus obtained extracts were defined and discussed.
Comparative Metabolite Profiling of Four Polyphenol Rich Morus Leaves extracts in Relation to their Antibiofilm Activity against Enterococcus faecalis
Authors: Salem Mohamed A. , Salama Maha M , Ezzat Shahira M. , Hashem Yomna A. 
Published: July 2022

Abstract
Enterococci are a common cause of urinary tract infections. The severity of enterococcal infections is associated with their ability to form biofilm. Morus leaves are known as a natural antibacterial, however, their antibiofilm activity against enterococcus remains unveiled. This study aimed to evaluate the ability of four polyphenol-rich Morus leaves extracts (Morus nigra, M. rubra, M. macroura, and M. alba) to inhibit biofilm formed by enterococcal clinical isolates in relation to their metabolic profiling. Results revealed that 48% of the isolates formed strong biofilm, 28% formed moderate biofilm, 20% formed weak biofilm and only 4% did not form a biofilm, the strong biofilm-forming isolates were E. faecalis and hence were chosen for this study. The antibiofilm activity of the four polyphenol-rich Morus leaves extracts revealed that the M. nigra extract exhibited the highest percentage of biofilm inhibition followed by M. rubra then M. macroura and the least inhibition was detected in M. alba, results are in accordance with the phenolic and flavonoid content of each extract. UPLC-ESI-MS/MS identified 61 polyphenolic compounds in the four extracts. Further, multivariate analysis confirmed clear segregation of M. nigra from the other species suggesting disparity in its metabolome, with accumulation of flavonoids, anthocyanidins, phenolic acids and coumarin derivatives. Quercetin and kaempferol glycosides were found to be positively and significantly correlated. In conclusion, M. nigra ethanolic extracts showed the highest phenolic content and antibiofilm activity and they could be developed as a complementary treatment for the development of antimicrobial agents.
Analysis and comparison of tear protein profiles in dogs using different tear collection methods
Authors: Ritchoo Sudpatchara , Havanapan Phattara-orn , Phungthanom Nuanwan , Rucksaken Rucksak , Muikaew Rattana , Sussadee Metita 
Published: July 2022

Abstract
Background: Tear proteomic analysis has become an important tool in medical and veterinary research. The tear collection method could influence the tear protein profile. This study aims to evaluate the protein profiles of dog tears collected using microcapillary tubes (MT), Schirmer tear strips (ST), and ophthalmic sponges (OS).Methods: The tear samples were collected using MT, ST, and OS. Tear protein profiles were analyzed using two-dimensional electrophoresis (2-DE) and the different protein spots' expression was compared. Fourteen protein spots were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS).Results: Tear protein concentrations ranged from 2.80 to 4.03 μg/μL, with no statistically significant differences among collection methods. Protein expression in each collection method differed in terms of both the number and intensity of the spots. There were 249, 327, and 330 protein spots found from tears collected with MT, ST, and OS, respectively. The proteins albumin, haptoglobin, and lactoferrin identified from OS were found to have higher spot intensities than other methods of collection. The use of MT demonstrated the downregulation of nine proteins.Conclusions: The recent study supported that tear protein analysis is affected by different tear collection methods. Although ST is commonly used for tear collection, it provides insufficient information to study particular tear proteins.
Analysis of Efficiency and Linearity of Cascode Class-E Power Amplifier with Reconfigurable Filter Network for wirelesses Application
Authors: seema seema , Kumar Suresh 
Published: July 2022

Abstract

High gain and high-efficiency Cascode Class-E power amplifier (PA) with Reconfigurable Filter Network for wireless application system using 0.25µm GaN technology is presented in the paper. Cascode Class-E PA is combined with a transmission line and Micro strip inter digital capacitor (MICAP) to illustrate a harmonic balance matching technique. The PA is composed of two stages and adopts a current source inter digital microwave capacitor (CSTIMC) and Cascode Class-E. This technique also enhances the resonant circuits band properties. Micro strip inter digital capacitor (MICAP) helps to enhance an internal matching design. For maximum output power, drain efficiency, and PAE the fundamental and second harmonic impedances are to be accurately improved for the particular operating frequency. The results represent maximum output power of 43.1dBm, drain efficiency of 74.5 % and power added efficiency (PAE) of 67.8 %. The fundamental and second harmonic balance levels are adjusted at -21dBc to -16dBc. The simulated and the measured results of the proposed power amplifier show the right consistency.
Association of plant-based diet and estimated glomerular filtration rate in Iranian Kurdish population: The RaNCD Cohort Study
Authors: Moloudpour Behrooz , Jam Samira Arbabi , Darbandi Mitra , Janati Ali , Gholizadeh Masoumeh , Najafi Farid , Pasdar Yahya 
Published: July 2022

Abstract
Background: Plant-based diet has antioxidant and anti-inflammatory properties. Therefore, this hypothesis is raised that following a plant-based diet may improve the functioning of the kidneys. The aim of study was to determine the association between plant-based diet index (PDI) and chronic kidney disease (CKD).Methods: This cross-sectional study was conducted using data from Ravansar non-communicable diseases (RaNCD) cohort study on 9,746 participants aged 35-65 years, in western Iran. CKD was assessed by glomerular filtration rate (eGFR) using the Modification of Diet in Renal Disease (MDRD) equation. The PDI was calculated based on food intake using a food frequency questionnaire (FFQ), and The food items were distributed into 18 food groups. We used multivariable logistic regression models to estimate odds ratio (ORs).Results: 1,058 (10.86%) of participants had CKD (eGFR<60 ml/min/1.73 m2), and the mean of PDI was 54.22± 6.68. The mean of eGFR in the group with high PDI score was significantly higher than the group with low PDI score (forth quartile: 79.20± 0.36 vs. first quartile: 72.95± 0.31, P<0.001). Adherence to a plant-based diet was more prevalent in the higher socioeconomic status (P<0.001). After adjusting for potential confounders, the odds of CKD in the third and fourth quartile of PDI was 25% (OR: 0.75; 95% CI: 0.62- 0.91) and 39% (OR: 0.61; 95% CI: 0.48- 0.78, P trend<0.001) lower than the first quartile, respectively.Conclusions: The findings of this study suggest that having a plant-based diet may be preventing the development of CDK. However, further studies are recommended.
The effect of crocin on phenotype switching of murine macrophages depends on their polarization state at the time of exposure to crocin
Authors: Abdi Hakimeh , Roshanravan Marjan , Hosseinzadeh Hossein , mosaffa fatemeh 
Published: July 2022

Abstract
Background
Macrophages have plastic phenotypes. M1 macrophages release inflammatory cytokines and have microbicidal activities. M2 cells release anti-inflammatory cytokines and repair tissue damages. The imbalance between M1 and M2 responses has an important effect on many pathological events. Crocin is an antioxidant and can eliminate free radicals, the properties involved in its anti-inflammatory capacities. We investigated the effects of crocin on macrophages phenotype switching in view of their primary activation states.
Methods
The effect of crocin different concentrations on macrophage viability was evaluated by MTT. TNF-α and IL-6 secretion as well as iNOS/Arg-I ratio were measured in cells treated with crocin and or LPS + INF-γ (M1 inducers). The cytokine secretion levels and the ratio were also determined in cells concurrently treated with crocin and LPS + INF-γ or cells pretreated with crocin before M1 induction.
Results
Crocin did not show any toxicity at a concentration of 500 µM and lower. When M0 uncommitted macrophages were exposed to crocin (25–100 µM), it raised some M1 activity indicators including iNOS/Arg-I ratio and TNF-α secretion, but not IL-6. Crocin in concurrent treatment with LPS + INF-γ could halt raising in M1 indicators, iNOS/Arg-I ratio and TNF-α secretion. Pretreatment of cells by crocin before the addition of LPS + INF-γ, could not converse the M1 induction in macrophages and caused a further increase in iNOS/Arg-I ratio and TNF-α secretion.
Conclusion
It seems that crocin modulatory effects on macrophage M1/M2 phenotype switching at least partly depends on the presence or absence of inflammatory mediators and accordingly the preliminary state of macrophage commitment.
Tumor Doubling Time as Preoperative Predictor of Malignancy and Recurrence in Newly Diagnosed Meningioma
Authors: Miwa Yusuke , Sugawara Takashi , Kobayashi Daisuke , Maehara Taketoshi 
Published: July 2022

Abstract
Objective
Most meningiomas are benign and the indications for surgery are determined by size and symptoms, but some are malignant and have a high recurrence rate. Currently, no preoperative prognostic factors have been established. The purpose of this study was to investigate whether Tumor doubling time (Td) is useful in predicting tumor prognosis.
Methods
Patients who underwent surgery for newly diagnosed meningioma at our hospital between 2007 and 2021 with preoperative magnetic resonance (MR) imaging evaluation over a period of six months were included in this study. We calculated the Td from the preoperative MR images and examined the correlation between Td and WHO grade, MIB-1 SI and other conditions.
Results
A total of 269 newly diagnosed meningiomas were operated in the period, of which 62 met inclusion criteria. The median Td was 1082 days (54-8579 days) and MIB-1 SI was 2.45% (0.7–14.6%). Td and MIB-1 SI had a negative correlation (r=-0.319, p = 0.0122). MIB-1 SI was higher in patients with Td < 3 years than in patients with Td > = 3 (p = 0.005), and the incidence of high WHO grade (grade2) was higher in patients with Td < 1 year than in patients with Td > = 1 (p = 0.014).
Conclusions
Meningiomas with Td of less than 3 years had significantly higher MIB-1 SI, and tumors with Td of less than 1 year had higher likelihood of malignancy. Therefore, early treatment should be considered for the patients with short Td meningioma even if asymptomatic and further consideration could be given to radical resection at the time of surgery.
SARS-CoV-2-related intestinal infection: virological and histological evaluation of intestinal samples
Authors: Cuicchi Dajana , Gabrielli Liliana , Tardio Maria Lucia , Rossini Giada , D’Errico Antonietta , Viale PierLuigi , Lazzarotto Tiziana , Poggioli Gilberto 
Published: July 2022

Abstract
Purpose: Several observations have collectively suggested that enteric infections can occur in COVID-19 patients. However, the detection of viral RNA in gastrointestinal tissue samples has not been adequately investigated. Methods: Intestinal mucosal samples obtained by rectal biopsy in COVID-19 patients with mild to moderate disease and from surgical specimens in COVID-19 patients who underwent intestinal resection were analysed with the primary objective of evaluating both SARS-CoV-2 RNA using real-time RT-PCR and its histological features. Results: Viral RNA was not detectable in any of the rectal biopsies performed (0/53). Histological examination showed no enterocyte damage, but slight edema of the lamina propria with mild inflammatory lymphoplasmacytic infiltration. Viral RNA was detected in 2 surgical specimens of the 6 examined, both of which were from patients with active neoplastic disease. Histological examination also pointed out abundant macrophages, granulocytes and plasmacells infiltranting the muscular layer and adipose tissue, and focal vasculitis. Conclusion: A weakened immune system, induced by neoplastic disease, could increase the risk of intestinal infection of SARS-CoV-2; mild-moderate COVID-19 may not be associated with rectal infection by the virus. More comprehensive autopsies or surgical specimens are needed to provide histological evidence of intestinal infection.
Source-specific risks apportionment and critical sources identification of potentially toxic elements in arable soils combining integrated positive matrix factorization model with multivariate analysis and machine learning classifiers: a case study of the Pannonia Basin
Authors: Škrbić Biljana D. , Buljovčić Maja , Antić Igor , Vágvölgyi Csaba 
Published: July 2022

Abstract
Understanding the source-specific ecological and human health risks and critical sources of arable soils by potential toxic elements (PTEs) is significant for urban contamination control and management. Comprehensive characterization of the arable soils used for organic and conventional agricultural management practice has been done for the first time in the Pannonian Basin, relating the levels of PTEs: As, Cd, Co, Cr, Cu, Ni, Zn, Pb and physicochemical characteristics. Pollution indices such as Igeo values for conventional agricultural management practice suggested that the investigated soil samples can be classified as ”unpolluted” or “moderate”, while for organic agricultural management practice samples were classified as “uncontaminated” to “heavy contaminated”. Integrated approaches applying Spearman correlation, positive matrix factorization, and multivariate analysis (PCA) were used to investigate relationships among PTEs and physicochemical properties of soils. PCA revealed that the identified PTEs’ pattern specified their distribution between soil samples intended for organic and conventional agricultural management practice while five different machine learning classifiers: Support Vector Machine, Bayesian Network, Multilayer Perceptron, Random Forest, and J48 which were used to develop test models intended to determine the type of soil agricultural practice have justified their high reliability to distinguish two types of management practice. Three main sources of PTEs were identified: natural, antropogenic, and agricultural sources, which accounted for 63.70%, 19.10%, and 17.20% of the total PTEs content, respectively. According to the occupational health risk assessed for the first time in studied agricultural region no potential non-carcinogenic risk is evidenced, while cancerogenic one was 8.41E-04 indicating potential risk.
TGF-β signaling pathway-based model to predict prognosis and immunity in osteosarcoma patients
Authors: Wu Zhouwei , Han Tao , Shen Zhihao , Xuan Jiangwei , Wang Xinwei 
Published: July 2022

Abstract
Background
The prognosis of patients with metastatic osteosarcoma is very poor, with long-term survival in < 20%. Transforming growth factor-β (TGF-β) playing an important role in the metastasis and prognosis of osteosarcoma patients. The aim of our study was to develop a TGF-β signaling pathway-based model to predict prognosis and immune status in osteosarcoma patients.
Methods
TGF-β related genes were comprehensively collected from several databases. The training cohort was downloaded from the TARGET database, while the validation cohort was extracted from GEO database. We identified differentially expressed TGF-β related genes between osteosarcoma patients with or without metastatic. We conducted cox regression to identify prognostic genes and developed the TGF-β based risk score. Then, we constructed a nomogram based on TGF-β based risk score, and conducted enrichment analysis to evaluate related genes and immune microenvironment. We used western blotting to detect the expression of genes. Statistical analysis was conducted by using R software and GraphPad Prism.
Results
We found four TGF-β related genes (MAPK1, MYC, PML and SLC2A8) which were associated with prognosis. Then we built a TGF-β based risk score for prognosis prediction and validated the good performance of this risk score system. We also developed a nomogram based on this risk score. In addition, we found cancer immunity was quilt different in low- and high-risk groups.
Conclusion
A novel risk score based on TGF-β was developed and can be used to predict prognosis time for osteosarcoma patients. Our research may also open an avenue for future studies of the relationships between TGF-β related genes and immunity.
Future tree survival in European natural forests depends on understorey diversity
Authors: Billing Maik , Thonicke Kirsten , Sakschewski Boris , von Bloh Werner , Walz Ariane 
Published: July 2022

Abstract
Climate change heavily threatens forest ecosystems worldwide and there is urgent need to understand what controls tree survival and forests stability. There is evidence that biodiversity can enhance ecosystem stability1,2, however it remains largely unclear whether this also holds for climate change and what aspects of biodiversity might be most important.Here we apply machine learning to outputs of a flexible-trait Dynamic Global Vegetation Model to unravel the effects of enhanced functional tree trait diversity and its sub-components on climate-change resistance of temperate forests (video abstract).We find that functional tree trait diversity enhances forest resistance. We explain this with 1. stronger complementarity effects (~25% importance) especially improving the survival of smaller trees up to +16.8% (±1.6%) and 2. environmental and competitive filtering of trees better adapted to future climate (40%-87% importance).We conclude that functionally diverse forests better resist and adapt to future conditions. In this context, we especially highlight the role of functionally diverse understoreys as they provide the fundament for better survival of young trees and filtering of resistant trees in the future.
A proteomic screen of Ty1 integrase partners identifies the protein kinase CK2 as a regulator of Ty1 retrotransposition
Authors: Barkova Anastasia , Adhya Indranil , Conesa Christine , Asif-Laidin Amna , Bonnet Amandine , Rabut Elise , Chagneau Carine , Lesage Pascale , Acker Joël 
Published: July 2022

Abstract
Background. Transposable elements are ubiquitous and play a fundamental role in shaping genomes during evolution. Since excessive transposition can be mutagenic, mechanisms exist in the cells to keep these mobile elements under control. Although many cellular factors regulating the mobility of the retrovirus-like transposon Ty1 in Saccharomyces cerevisiae have been identified in genetic screens, only very few of them interact physically with Ty1 integrase (IN).Results. Here, we perform a proteomic screen to establish Ty1 IN interactome. Among the 265 potential interacting partners, we focus our study on the conserved CK2 kinase. We confirm the interaction between IN and CK2, demonstrate that IN is a substrate of CK2 in vitro and identify the modified residues. We find that Ty1 IN is phosphorylated in vivo and that these modifications are dependent in part on CK2. No significant change in Ty1 retromobility could be observed when we introduce phospho-ablative mutations that prevent IN phosphorylation by CK2 in vitro. However, the absence of CK2 holoenzyme results in a strong stimulation of Ty1 retrotransposition, characterized by an increase in Ty1 mRNA and protein levels and a high accumulation of cDNA. ConclusionOur study highlights an important role of CK2 in the regulation of Ty1 retrotransposition. We provide the first evidence that Ty1 IN is post-translationally modified in vivo, as observed for retroviral INs, and demonstrate that CK2 strongly represses Ty1 mobility by inhibiting Ty1 transcription. The proteomic approach enabled the identification of many new Ty1 IN interacting partners, whose potential role in the control of Ty1 mobility will be interesting to study.