bluuebunny/crossref_metadata_2025_split huggingface dataset:
Description of Individualized Delirium Intervention in Intensive Care Unit (IDI-ICU) for critically ill patients delivered by mobile health care system
Authors: Zhang Shan , Ji Meihua , Cui Wei , Wu Ying 
Published: May 2022

Abstract

Background
Delirium is a preventable and reversible complication for intensive care unit patients, which can be linked to short-term and long-term negative outcomes. Early intervention to cope with risk factors of delirium are necessary. Yet no specific description of IDI-ICU following the Template for Intervention Description and Replication (TIDieR) were reported. This study aimed to describe individualized delirium intervention in intensive care unit (IDI-ICU) for critically ill patients.
Methods
This descriptive research describes the evidence-based ICU delirium interventions for improving cognitive load and adherence of nurses and reducing ICU delirium incidence. Therefore, both nurses and researchers would benefit from the replication of the interventions for clinical approach or experimental research.
Results
The TIDieR checklist improved the description of ICU delirium interventions, including several key features (duration, dose or intensity, essential processes, and tailoring) for improved implementation of the intervention. The IDI-ICU library includes delirium screening, risk factor assessment, ABCDEF bundle intervention. We (1) standardized the flow chart of ICU delirium assessment tools; (2) formed an evaluation sheet of ICU delirium risk factors; and (3) translated the evidence-based ABCDEF bundle intervention into practice.
Conclusions
The TIDieR checklist provided a systematic approach for reporting the complex ICU delirium interventions delivered in a clinical interventional trial. Future well-designed RCTs are needed to examine the effectiveness of the IDI-ICU on improving cognitive load and adherence of nurses and reducing ICU delirium incidence and duration.
Non-centrosymmetric features in nanostructured MoS 2 and WS 2 exfoliated in liquid phase
Authors: Cardenas-Chirivi Gabriel , Navarrete Cristina , Lozano Diana , Herreño Cesar , Hernandez Yenny 
Published: May 2022

Abstract
Exfoliation in liquid phase of Transition Metal Dichalcogenides promotes the industrialization of low dimensional structures in photonic and material science applications, due to high nonlinear optical and piezoelectric responses on monolayers and few odd layers of these materials. Here, we report piezoelectricity and prospects of non-linear optics through Switching Spectroscopy Piezoresponse Force Microscopy (SS-PFM) and Fluorescence spectroscopy on MoS 2-and WS 2-flakes exfoliated in liquid phase. These features are intrinsically related to non-centrosymmetric structures and depend on the number of layers. SS-PFM signal in a small region reveals that the strength of AFM tip deflection was considerable just for an odd number of layers and negligible for even layers.Thin films were evaluated with ellipsometric measurements by constructing models mixing mono and few layers, resulting in a detailed description of the optical and surface properties of the nanostructures studied.
The amount of circulating DNA and the presence of TP53 mutations in plasma predict clinical outcome of renal cancer patients treated with immunotherapy and VEGFR-TKIs
Authors: Re Marzia Del , Crucitta Stefania , Paolieri Federico , Cucchiara Federico , Verzoni Elena , Bloise Francesco , Ciampi Raffaele , Mercinelli Chiara , Capuano Annalisa , Martinetti Antonia , Procopio Giuseppe , Galli Luca , Porta Camillo , Bracarda Sergio , Danesi Romano 
Published: May 2022

Abstract
Background Despite the increasing treatments options, reliable biomarkers of response are still missing for patients affected by metastatic renal cell carcinoma (mRCC).Methods Patients affected by mRCC undergoing standard first line treatment were enrolled. Twelve ml of blood were drawn at treatment baseline and circulating free DNA (cfDNA) was extracted from plasma. Next-generation sequencing (NGS) analyses were performed on cfDNA using the Pan-Cancer Cell-Free Assay and clinical outcomes were correlated with liquid biopsy.Results A total of 48 patients were enrolled, 12 received immunotherapy and 36 received a vascular endothelial growth factor receptor (VEGFR) tyrosine kinase inhibitor (TKI). A cfDNA cut-off of 0.883 ng/ml stratified patients based on progression-free survival (PFS) and overall survival (OS) (p=0.001 and p=0.008, respectively). cfDNA amount was also correlated with best response (p=0.006). A further cfDNA cut-point divided patients into short, intermediate and long responders, with a PFS of 4.87 vs 9.13 vs 23.1 months, respectively (p<0.001). PFS resulted to be shorter in carriers of mutant TP53 compared to not carriers with a p=0.04. Patients with high cfDNA levels and TP53 mutant have the worst PFS, and patients with low cfDNA and no mutations in TP53 have the longer PFS (p=0.004).Conclusions The present study demonstrates that cfDNA and TP53 may be used as predictive biomarkers of response in mRCC.
Network Analysis Reveals Tumor Suppressor lncRNA GAS5 Acts as a Double-edged Sword in Gastric Cancer
Authors: Gupta Shantanu , Panda Pritam , Hashimoto Ronaldo , Ahuja Rajeev 
Published: May 2022

Abstract
The lncRNA GAS5 acts as a tumor suppressor and is downregulated in gastric cancer (GC). In contrast, E2F1, an important transcription factor, and tumor promoter, directly inhibits miR-34c expression in GC cell lines. Furthermore, in the corresponding GC cell lines, lncRNA GAS5 directly targets E2F1. However, lncRNA GAS5 and miR-34c remain to be studied in conjunction with GC. Here, we present a dynamic Boolean network to classify gene regulation between these two non-coding RNAs in GC. This is the first study to show that lncRNA GAS5 can positively regulate miR-34c in GC through a previously unknown molecular pathway coupling lncRNA/miRNA. We compared our network to several in-vivo/in-vitro experiments and obtained an excellent agreement. We revealed that lncRNA GAS5 regulates miR-34c by targeting E2F1. Additionally, we found that lncRNA GAS5, independently of p53, inhibits GC proliferation through the ATM/p38 MAPK signaling pathway. These findings open a new avenue for GC treatment.
Intraoperative factors influencing transcranial motor-evoked potential measurements using higher frequency multi-train stimulation in adolescent scoliosis surgery: A prospective observational study
Authors: Toki Takayuki , Fujita Noriaki , Ichikawa Tomohiro , Ochi Noriki , Yokota Isao , Sudo Hideki , Morimoto Yuji 
Published: May 2022

Abstract
Measurement of transcranial motor-evoked potentials (TcMEPs) in scoliosis surgery has been reported to be a sensitive and specific method for detecting postoperative new neurological defects. However, the influence of intraoperative factors makes the interpretation of TcMEPs difficult. This study aimed to investigate the influence of intraoperative factors on TcMEP amplitude using higher frequency multi-train stimulation (hfMTS) in adolescent scoliosis surgery, and the complications of hfMTS. We included 50 patients and 706 TcMEP measurements in the analysis. The median number of train stimuli was 13 [IQR: 11, 14], and the median TcMEP amplitude was 3.25 [IQR: 2.50, 4.18] mV. Based on a mixed-effect model with random intercept for TcMEP amplitude, we estimated the mean difference and its 95% confidence interval and the predicted mean difference and its confidence interval by the interquartile range of each factor. The predicted mean differences in TcMEP amplitude were clinically limited compared to the actual TcMEP amplitude, suggesting that each factor had a limited effect on TcMEP amplitude. No intraoperative bite injuries or seizures were observed. The use of hfMTS in adolescent scoliosis surgery may provide sufficient TcMEP amplitude without the influence of intraoperative factors or complications.
Control of blood loss during giant upper extremity tumor resection by interventional methods
Authors: Huo Fei , Liang Hansheng , Feng Yi 
Published: May 2022

Abstract
Background:The purposes of this retrospective study were to determine the efficacy of interventional methods in control of intraoperative blood losses and investigate the perioperative complications.Methods:The cases of 44 patients in whom a giant upper extremity tumor had been operated between 2008 and 2022 were analyzed retrospectively. Twenty-nine patients were treated with interventional methods(Group A)compared with fifteen patients treated without the interventional methods(Group B).According to the different intervention methods, group A was further broken down into Group C(the combination of the balloon occlusion and transarterial embolization(TAE),n=11) and Group D(the TAE group,n=18).According to the days of TAE prior to the surgery, Goup D was broken into Group E(the same day of TAE prior surgery) and Group F(more days of TAE prior to surgery).The demographic features ,the blood loss and ICU(intensive care unit) admission rate and the application of vasopressors during the surgery were kept and compared.Results: Clinical records of 44 cases who were diagnosed with giant upper extremity tumor and underwent surgery were collected. The number of groups A,B,C,D,E and F were 29, 15, 11,18,7,11.The maximum diameter of the tumor(cm) in Group B(without interventional group) was significant larger than that of o the Group A(with interventional group)(12.61±4.47 vs 9.78±3.71,P=0.031<0.05).The transfusion of blood plasma(ml) of Group B is also higher than that of Group A(425.33±476.20 vs 425.33±476.20,P=0.021<0.05).Although the blood loss(ml) didn’t differ significantly between Group A and Group B(467.93±302.08 vs 1150±1424.15,p=0.087>0.05 ).But when we defined massive bleeding as the blood loss over 1000 mls,we found that the massive bleeding rate in Group A is lower than that in Group B(6.9% vs 46.47%,p=0.004<0.05).The rate of minors(patient’s age less than 18) in Group C was also significantly higher than that in Group D(27.7% vs 0.00%,p=0.045<0.05).The amount of transfusion of RBC(ml) in Group C was also significantly higher than that of Group D(458.18±292.22 vs 164.44±224.03,p=0.021<0.05). We didn’t find obvious perioperative complications in our patients.Conclusions: Interventional methods decrease the size of the tumor, and decrease the blood loss and the amount of blood transfusion in patients with giant upper extremity tumor. For senile patients with primary vessel problems, the placement of endovascular balloon should be more cautious.There exits no obvious complications perioperatively.
Comparative genomic analysis of the genus Marinomonas and taxonomic study of Marinomonas algarum sp. nov., isolated from red algae Gelidium amansii
Authors: Xue Jian-Heng , Zhang Bei-Ning , Zhang Feng , Liu Ying-Ying , Wu Wen-Jie , Wu Zhao-Ming , Si Yue , Yang Peng-Xi , Xing Xiang , Zhao Li-Hua 
Published: May 2022

Abstract
Members of the genus Marinomonas are known for their environmental adaptation and metabolically versatility, with abundant proteins associated with antifreeze, osmotic pressure resistance, carbohydrase and multiple secondary metabolites. Comparative genomic analysis focusing on secondary metabolites and orthologue proteins were conducted with 30 reference genome sequences in genus Marinomonas. In this study, a Gram-stain-negative, rod-shaped, non-flagellated and strictly aerobic bacterium, designated as strain E8T, was isolated from the red algae (Gelidium amansii) in the coastal of Weihai, China. Optimal growth of the strain E8T was observed at temperatures 25–30 ℃, pH 6.5-8.0 and 1–3% (w/v) NaCl. The DNA G + C content was 42.8 mol%. The predominant quinone was Q-8 and the major fatty acids were C16:0, summed feature 3 and summed feature 8. The main polar lipids were phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Based on data obtained from this polyphasic taxonomic study, strain E8T belongs to a novel species of the genus Marinomonas, for which the name Marinomonas algarum is proposed. The type strain is E8T (= KCTC 92201T = MCCC 1K07070T).
Time Series Observations and Recent Anomalies of the Sea Surface Temperatures in the Cilician Basin
Authors: Deliceirmak Cagri , Salihoglu Ilkay 
Published: May 2022

Abstract
Sea Surface Temperature (SST) is one of the fundamental physical oceanographic parameters to understand, determine and predict ocean-atmosphere interactions. SST defines and influences local and global climate regimes, oceanic and atmospheric circulation, weather patterns, and regional phenomena. Almost ninety per cent of the additional heat energy caused by greenhouse gasses is absorbed by the ocean. Compared to the pre-industrial era, the mean annual SST of the Mediterranean Sea is increased by about 1.0 ℃, especially with a higher rate in the last decades. Since the 1990s, a gradually increasing trend of SST was observed in the Mediterranean Sea, while the highest SST warming was recorded in the Levantine Basin of the Eastern Mediterranean Sea. The fifth-generation atmospheric reanalysis (ERA5) data sets of the European Centre of the Medium-Range Weather Forecast (ECMWF) and the time-series of observational meteorological data sets of the Kyrenia Meteorological Station are used. This study aimed to compare and validate the reanalysis and observational data sets and analyse SST trends in the southern Cilician Basin between 1995 and 2020. Our results revealed about 1.1 ℃ of SST warming in the region during the last 25 years. The annual mean SST was calculated as ~ 22.20 ℃. Continuous positive SST anomalies, recorded since 2010, are another significant outcome of the study, which shall be crucial for monitoring and predicting future climatic events.
Microwave treatment prevents cytomegalovirus transmission to preterm infants through human milk
Authors: Mizuno Katsumi , Kohda Chikara , Den Hiroki , Mizuno Noriko , Hijikata Minako , Yamamoto Makoto 
Published: May 2022

Abstract
Background: Mother’s own milk (MOM) is the best nutrition for preterm infants; however, milk from cytomegalovirus (CMV)-seropositive mothers may cause the transmission of the virus to the infants. As extremely preterm infants are at a risk of developing serious conditions due to CMV infection, even MOM is sometimes pasteurized. Previously, we demonstrated that microwave heating of 50 mL formula milk containing CMV at 500 W for 40s prevented CMV infection in living cells. However, we do not know if this is the case with reactivated CMV in human milk (HM). Therefore, we investigated if microwave heating of HM would be effective in preventing CMV infection in living cells.Methods: Twenty-three CMV-IgG-positive and four CMV-IgG-negative mothers who donated HM to the human milk bank during 6 to 16 weeks postpartum were enrolled. The HM was treated with microwave at 500 W. HFL-III cells were seeded in culture dishes. Aliquots of HM samples subjected to microwave was inoculated onto susceptible cell monolayers. Infectious vial titer was measured by the median tissue culture infectious Dose (TCID50) assay. Results: The TCID50 assay was decreased in five out of eight 100-mL samples after microwave at 200W for 60 s. The CMV plaque assay was negative in three, decreased in eight, and no change in six 100-mL samples, and negative in four and decreased in eight 50-mL samples.Conclusions: Microwave heating of HM at low settings could decrease CMV transmission through HM, however, to obtain entire prevention is difficult when loss of bioactive properties is considered.
Developing feedback literacy in physiotherapy students: a pilot study
Authors: Chong Doris Yin Kei , Chow Sanya Helen , Tsang William Wai Nam 
Published: May 2022

Abstract
Background: The ability to meaningfully use feedback is an important life skill especially in the healthcare industry. Traditionally, feedback has been viewed as rather one-way information. A new feedback literacy paradigm stresses the importance of student agency and reconceptualising feedback as a sense-making process. However, research on how to improve feedback literacy is still in the development stage.Methods: This mixed method pilot study explored the effectiveness of a novel feedback literacy workshop for physiotherapy students. Participants’ level of feedback literacy was measured before and after the workshop using a customised questionnaire. Participants also shared in a focus group their perceptions and experiences of feedback before the workshop, as well as anticipated changes after the workshop. Results: Thematic analyses from the focus group illustrated mixed reactions to the workshop. While there were mismatches of expectations about the workshop, participants anticipated positive change resulting from it. They also demonstrated feedback seeking behaviour after the workshop, a feature that the new paradigm aims to promote. However, owing to the small and possibly biased sample, no statistically significant difference was found between the pre-test and post-test feedback literacy score in the Wilcoxon signed rank test (P>0.05).Conclusions: There is potential for a curriculum-embedded and structured feedback literacy training to improve feedback literacy in physiotherapy students. The pilot results provide insights on workshop designs and implementation strategies for a future larger scale study.
Hyperglycemia Aggravates Blood–Brain Barrier Disruption Following Diffuse Axonal Injury by Increasing the Levels of Inflammatory Mediators through the PPARγ/Caveolin-1/TLR4 Pathway
Authors: Wei Xing , Zhou Yaqing , Song Jinning , Zhao Junjie , Huang Tingqin , Zhang Ming , Zhao Yonglin 
Published: May 2022

Abstract
Hyperglycemia aggravates brain damage after diffuse axonal injury (DAI), but the underlying mechanisms are not fully defined. In this study, we aimed to investigate a possible role for hyperglycemia in the disruption of blood–brain barrier (BBB) integrity in a rat model of DAI and the underlying mechanisms. Accordingly, 50% glucose was intraperitoneally injected after DAI to establish the hyperglycemia model. Hyperglycemia treatment aggravated neurological impairment and axonal injury, increased cell apoptosis and glial activation, and promoted the release of inflammatory factors, including TNF-α, IL-1β, and IL-6. It also exacerbated BBB disruption and decreased the expression of tight junction-associated proteins, including ZO-1, claudin-5, and occludin-1, whereas the PPARγ agonist rosiglitazone (RSG) had the opposite effects. An in vitro BBB model was established by a monolayer of human microvascular endothelial cells (HBMECs). Hyperglycemia induction worsened the loss of BBB integrity induced by oxygen and glucose deprivation (OGD) by increasing the release of inflammatory factors and decreasing the expression of tight junction-associated proteins. Hyperglycemia further reduced the expression of PPARγ and caveolin-1, which significantly decreased after DAI and OGD. Hyperglycemia also further increased the expression of toll-like receptor 4 (TLR4), which significantly increased after OGD. Subsequently, the PPARγ agonist RSG increased caveolin-1 expression and decreased TLR4 expression and inflammatory factor levels. In contrast, caveolin-1 siRNA abrogated the protective effects of RSG in the in vitro BBB model of hyperglycemia by increasing TLR4 and Myd88 expression and the levels of inflammatory factors, including TNF-α, IL-1β, and IL-6. Collectively, we demonstrated that hyperglycemia was involved in mediating secondary injury after DAI by disrupting BBB integrity by inducing inflammation through the PPARγ/caveolin-1/TLR4 pathway.
Correlation analysis of UGT1A1 gene and neonatal hyperbilirubinemia based on MLPA-NGS technique
Authors: Yin Di , Yang Yongchen , Zhang Huating , Wang Xuefang , Guan Kunyu , Zhang Hong , Gong Xiaohui , Wu Shengnan , Hu Yong 
Published: May 2022

Abstract
Objective: This study aims to explore whether there was a correlation between unexplained severe neonatal hyperbilirubinemia and the following six gene pathogenic variants: c.211G>A of UGT1A1 gene, c.388A>G, c.597C>T, c.521T>C of SLCO1B1 gene, c.175T>C, and c.7G>A of BLVRA gene.Methods: This was a case-control study. The hyperbilirubinemia group, including 65 neonates with unexplained severe hyperbilirubinemia (serum total bilirubin level ≥342 μmol·L-1), was hospitalized in the neonatal intensive care unit of Shanghai Children's Hospital from January 2019 to September 2020. The control group included 52 neonates with birth defects and serum total bilirubin level £221 μmol·L-1. The six gene pathogenic variants mentioned above were investigated using MLPA-NGS technique. Pathogenic variants and allele frequencies were compared between the two groups.Results: Compared with previous sequencing results, the concordance rate of c.211G>A was 98.3% (115/117), while all other pathogenic variants had a concordance rate of 100%. The pathogenic variants frequencies were as follows: c.388A>G of SLCO1B1 gene (93.84%)>c.7G>A of BLVRA gene (89.23%)> c.597C>T of SLCO1B1 gene (81.54%)> c.211G>A of UGT1A1 gene (69.23%)> c.521T>C of SLCO1B1 gene (26.15%)> c.175T>C of BLVRA gene (18.46%). A allele frequency of c.211G>A of hyperbilirubinemia group was 45.38%, while A allele frequency of control group was 21.15%. The differences were statistically significant, with c2=14.988, P<0.001. No significant difference was observed in allele frequency of other gene pathogenic variants between the two groups, with P>0.05. Binary logistic regression results revealed that c.211G>A of UGT1A1 gene was a high-risk factor for unexplained severe neonatal hyperbilirubinemia, with OR=4.233, 95% CI: 1.896-9.451. No significant difference was observed in serum total bilirubin levels between mutation types.Conclusions: c.211G>A of UGT1A1 gene was significantly correlated with unexplained severe neonatal hyperbilirubinemia and was a high-risk factor for the disease. MLPA-NGS technique was a highly accurate, rapid, and cost-effective method in identifying common and known gene loci of severe neonatal hyperbilirubinemia. It can be employed as a point mutation screening approach for detecting hyperbilirubinemia-related pathogenic variants.
Crystal chemistry and origin of epidote-(Sr) in alkaline rocks of the teschenite association (Silesian Unit, Outer Western Carpathians, Czech Republic)
Authors: Kropáč Kamil , Dolníček Zdeněk , Uher Pavel , Buriánek David , Urubek Tomáš 
Published: May 2022

Abstract
A new occurrence of epidote-(Sr) [CaSrAl2Fe3+(Si2O7)(SiO4)(OH)], Sr-REE-rich epidote and Sr-rich allanite-(Ce) is located in Lower Cretaceous, Sr-rich hydrothermally altered leucocratic dykes penetrating alkaline igneous rocks (teschenites) near the Nový Jičín town (the Silesian Unit, Outer Western Carpathians). The dykes contain phenocrysts of pyroxene, amphibole, biotite, fluorapatite and dominant felsic groundmass consisting mostly of alkali feldspars and zeolites (analcime, natrolite and thomsonite-Ca). Accessory minerals include Ti-rich magnetite, prehnite, chamosite, pyrite, calcite, (OH, F)-rich grossular, epidote-group minerals, HFSE-, REE-rich minerals, Sr-rich baryte and slawsonite. The Sr-rich epidote forms columnar crystals or irregular aggregates which are mostly spatially related to chamosite pseudomorphs; it contains 0.15–0.81 apfu Sr, <0.53 apfu REE; Fe3+/(Fe3+ + Al) = 0.16–0.48. The Sr2+ substitutes Ca2+ in the A2 site by a coupled substitution involving other A2 (REE3+, Y3+, Th4+) or M (Al3+, Fe3+, Fe2+) cations. The Sr-rich epidote crystallized from hydrothermal solutions, probably at temperatures between ~250–300 °C, during cooling of the host rock. The dykes show higher Sr contents (5680–7830 ppm) and 87Sr/86Sri(120 Ma) ratios (~0.7046–0.7047) compared to host mesocratic teschenites (1310–1470 ppm Sr and ~0.7038–0.7045, respectively). The Sr isotopes indicate origin of most Sr from primary magmatic plagioclase in parent teschenite. Nevertheless, there participated fluids of external origin, derived from the Lower Cretaceous seawater or diagenetic waters related to associated siliciclastic sediments with 87Sr/86Sri(120 Ma) = ~0.7073–0.7083. These more radiogenic sources contributed at least 6–21 % of the bulk Sr budget of the studied Sr-rich epidote-bearing leucocratic dykes.