bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effective Ligand Design: Zinc Complexes with Guanidine Hydroquinoline Ligands for Fast Lactide Polymerization and Chemical Recycling
Authors: Hermann Alina , Becker Tabea , Schäfer Martin A. , Hoffmann Alexander , Herres‐Pawlis Sonja 
Published: July 2022

AbstractIn this study, the synthesis of two new guanidine hydroquinoline ligands served as basis for six new zinc guanidine complexes. Two of these complexes showed very high activity in the lactide polymerization under industrial conditions. The lactide polymerization was demonstrated in solution and melt conditions observing high activity and molar masses up to 90 000 g mol−1. Density functional theory studies elucidated the high activity of the complexes associated with the influence of the ligand backbone and the use of triflate counterions. On the way towards a circular economy, polymerization and depolymerization go hand in hand. So far, guanidine complexes have only shown their good activity in the ring opening polymerization of esters, and guanidine complexes with pure N donors have not been tested in recycling processes. Herein, the excellent ability of zinc guanidine complexes to catalyze both polymerization and depolymerization was demonstrated. The two most promising zinc complexes efficiently mediated the methanolysis of polylactide into methyl lactate under mild reaction conditions.
Promoting Reversible Dissolution/Deposition of MnO2 for High‐Energy‐Density Zinc Batteries via Enhancing Cut‐Off Voltage
Authors: Ruan Pengchao , Xu Xilian , Zheng Dong , Chen Xianhong , Yin Xinyu , Liang Shuquan , Wu Xianwen , Shi Wenhui , Cao Xiehong , Zhou Jiang 
Published: July 2022

AbstractZn//MnO2 batteries based on the MnO2/Mn2+ conversion reaction mechanism featuring high energy density, safety, and affordable cost are promising in large‐scale energy storage application. Nonetheless, the continuous H+ intercalation at low potential reduces the average output voltage and the energy efficiency, impeding the development of the high‐performance zinc battery. In this work, a strategy was proposed of enhancing the cut‐off voltage from the perspective of electrochemical parameters, toward high energy efficiency and stable output voltage of the Zn//MnO2 battery. This strategy was beneficial to promoting MnO2 dissolution/deposition through the increase of acidity caused by the constant accumulation of MnO2 and inhibiting H+ (de)intercalation during cycling process, thereby improving the energy efficiency (83.5 %) along with the stable average output voltage (1.88 V) under the cut‐off voltage of 1.8 V. This work provides a new pathway to promote aqueous zinc batteries with high energy density and stable output voltage.
Single‐cell RNA sequencing reveals localized tumour ablation and intratumoural immunostimulant delivery potentiate T cell mediated tumour killing
Authors: Hoover Ashley R. , Liu Kaili , DeVette Christa I. , Krawic Jason R. , Medcalf Alexandra D. , West Connor L. , Hode Tomas , Lam Samuel S.K. , Welm Alana L. , Sun Xiao‐Hong , Hildebrand William H. , Chen Wei R. 
Published: July 2022

AbstractBackgroundMetastatic breast cancer poses great challenge in cancer treatment. N‐dihydrogalactochitosan (GC) is a novel immunoadjuvant that stimulates systemic immune responses when administered intratumourally following local tumour ablation. A combination of photothermal therapy (PTT) and GC, referred to as localized ablative immunotherapy (LAIT), extended animal survival and generates an activated B cell phenotype in MMTV‐PyMT mouse mammary tumour microenvironment (TME). However, how T cell populations respond to LAIT remains to be elucidated.MethodsUsing depletion antibodies, we studied the contributions of CD8+ and CD4+ T cells to the therapeutic effect of LAIT. Using single‐cell RNA‐sequencing (scRNAseq), we analysed tumour‐infiltrating T cell heterogeneity and dissected their transcriptomes upon treatments of PTT, GC, and LAIT (PTT+GC).ResultsLoss of CD8+ T cells after LAIT abrogated the therapeutic benefits of LAIT. Ten days after treatment, proportions of CD8+ and CD4+ T cells in untreated TME were 19.2% and 23.0%, respectively. Upon LAIT, both proportions were increased to 25.5% and 36.2%, respectively. In particular, LAIT increased the proportions of naïve and memory cells from a resting state to an activated state. LAIT consistently induced the expression of co‐stimulatory molecules, type I IFN responsive genes, and a series of antitumor cytokines, Ifng, Tnf, Il1, and Il17 in CD8+ and CD4+ T cells. LAIT also induced immune checkpoints Pdcd1, Ctla4, and Lag3 expression, consistent with T cell activation. Relevant to clinical translation, LAIT also upregulated genes in CD8+ and CD4+ T cells that positively correlated with extended survival of breast cancer patients.ConclusionsOverall, our results reveal that LAIT prompts immunological remodelling of T cells by inducing broad proinflammatory responses and inhibiting suppressive signalling to drive antitumour immunity.
WDR62 variants contribute to congenital heart disease by inhibiting cardiomyocyte proliferation
Authors: Hao Lili , Ma Jing , Wu Feizhen , Ma Xiaojing , Qian Maoxiang , Sheng Wei , Yan Tizhen , Tang Ning , Jiang Xin , Zhang Bowen , Xiao Deyong , Qian Yanyan , Zhang Jin , Jiang Nan , Zhou Wenhao , Chen Weicheng , Ma Duan , Huang Guoying 
Published: July 2022

AbstractBackgroundCongenital heart disease (CHD) is the most common birth defect and has high heritability. Although some susceptibility genes have been identified, the genetic basis underlying the majority of CHD cases is still undefined.MethodsA total of 1320 unrelated CHD patients were enrolled in our study. Exome‐wide association analysis between 37 tetralogy of Fallot (TOF) patients and 208 Han Chinese controls from the 1000 Genomes Project was performed to identify the novel candidate gene WD repeat‐containing protein 62 (WDR62). WDR62 variants were searched in another expanded set of 200 TOF patients by Sanger sequencing. Rescue experiments in zebrafish were conducted to observe the effects of WDR62 variants. The roles of WDR62 in heart development were examined in mouse models with Wdr62 deficiency. WDR62 variants were investigated in an additional 1083 CHD patients with similar heart phenotypes to knockout mice by multiplex PCR‐targeting sequencing. The cellular phenotypes of WDR62 deficiency and variants were tested in cardiomyocytes, and the molecular mechanisms were preliminarily explored by RNA‐seq and co‐immunoprecipitation.ResultsSeven WDR62 coding variants were identified in the 237 TOF patients and all were indicated to be loss of function variants. A total of 25 coding and 22 non‐coding WDR62 variants were identified in 80 (6%) of the 1320 CHD cases sequenced, with a higher proportion of WDR62 variation (8%) found in the ventricular septal defect (VSD) cohort. WDR62 deficiency resulted in a series of heart defects affecting the outflow tract and right ventricle in mouse models, including VSD as the major abnormality. Cell cycle arrest and an increased number of cells with multipolar spindles that inhibited proliferation were observed in cardiomyocytes with variants or knockdown of WDR62. WDR62 deficiency weakened the association between WDR62 and the cell cycle‐regulated kinase AURKA on spindle poles, reduced the phosphorylation of AURKA, and decreased expression of target genes related to cell cycle and spindle assembly shared by WDR62 and AURKA.ConclusionsWDR62 was identified as a novel susceptibility gene for CHD with high variant frequency. WDR62 was shown to participate in the cardiac development by affecting spindle assembly and cell cycle pathway in cardiomyocytes.
Examining older adults with neuroatypical conditions for MCI/dementia: Barriers and recommendations of the Neuroatypical Conditions Expert Consultative Panel
Authors: Janicki Matthew P. , Hendrix James A. , McCallion Philip ,   and the Neuroatypical Conditions Expert Consultative Panel
Published: July 2022

AbstractThe Neuroatypical Conditions Expert Consultative Panel composed of numerous clinical and academic experts was convened to examine barriers to the examination of cognitive impairment in adults with a variety of neuroatypical conditions. Neuroatypical conditions affect normative intellectual development and function (such as intellectual disability and intellectual disability with conjoint psychiatric conditions), thought, moods, and cognition (such as severe mental illness), communication functions (such as the autism spectrum and hearing/vision impairments), and brain and motor function (such as cerebral palsy and acquired or traumatic brain injury). The panel concluded that current federal guidance for the assessment of cognitive impairment for mild cognitive impairment (MCI) or dementia does not sufficiently include information as to how to assess such adults. In addition, it concluded that adults with these conditions (1) challenge clinicians when attempting to discern current behavior and function from that which was pre‐existing; (2) often have inherent comprehension and oral communication difficulties, motor task performance impediments, and difficulty with visuals; and (3) pose difficulties when assessed with standardized dementia measures and can benefit from the use of specialized instruments. The panel recommended that federal guidance be broadened to include adaptations of assessment practices to accommodate neuroatypical conditions; that educational packs be developed for clinicians about such conditions and on detecting and diagnosing MCI or dementia; and that research be expanded to produce more evidence‐based information on both assessing adults with neuroatypical conditions for later‐life adult cognitive diseases/disorders and planning post‐diagnostic care.
Icariside II suppresses the tumorigenesis and development of ovarian cancer by regulating miR‐144‐3p/IGF2R axis
Authors: Yuan Donglan , Guo Ting , Qian Hua , Ge Hongshan , Zhao Yinling , Huang Aihua , Wang Xiaosu , Cao Xiuhong , Zhu DanDan , He CuiQin , Yu Hong 
Published: July 2022

AbstractOvarian cancer is one of the three major gynecological malignancies. It has been reported that Icariside II was able to block the occurrence and development of ovarian cancer. However, the detailed mechanism by which Icariside II regulates the development of ovarian cancer is widely unknown. EdU staining and transwell assays were applied to detect the proliferation, migration, and invasion of ovarian cancer cells. Next, the relationship between miR‐144‐3p and IGF2R was verified by the dual‐luciferase reporter assay. Moreover, in vivo animal model was constructed to verify the effect of Icariside II on the development of ovarian cancer. Icariside II notably inhibited the proliferation, migration, and invasion and induced the apoptosis of ovarian cancer cells. Additionally, Icariside II markedly increased the level of miR‐144‐3p in ovarian cancer cells. Moreover, IGF2R was targeted by miR‐144‐3p directly. Icariside II significantly decreased the expression of IGF2R and the phosphorylation level of AKT and mTOR in ovarian cancer cells, which were partially reversed by miR‐144‐3p inhibitor. Meanwhile, Icariside II remarkably promoted the autophagy of ovarian cancer cells, as confirmed by the increased expression of Beclin‐1 and ATG‐5 and decreased expression of p62; however, co‐treatment with miR‐144‐3p inhibitor notably decreased autophagy. Furthermore, the result of animal study suggested Icariside II notably inhibited ovarian tumor growth as well. Collectively, Icariside II could suppress the tumorigenesis and development of ovarian cancer by promoting autophagy via miR‐144‐3p/IGF2R axis. These results may be beneficial for future studies on the use of Icariside II to treat ovarian cancer.
Effect of tramadol on apoptosis and synaptogenesis in hippocampal neurons: The possible role of µ‐opioid receptor
Authors: Hosseindoost Saereh , Akbarabadi Ardeshir , Sadat‐Shirazi Mitra‐Sadat , Mousavi Seyed Mojtaba , Khalifeh Solmaz , Mokri Azarakhsh , Hadjighassem Mahmoudreza , Zarrindast Mohammad‐Reza 
Published: July 2022

AbstractTramadol is a synthetic opioid with centrally acting analgesic activity that alleviates moderate to severe pain and treats withdrawal symptoms of the other opioids. Like other opioid drugs, tramadol abuse has adverse effects on central nervous system components. Chronic administration of tramadol induces maladaptive plasticity in brain structures responsible for cognitive function, such as the hippocampus. However, the mechanisms by which tramadol induces these alternations are not entirely understood. Here, we examine the effect of tramadol on apoptosis and synaptogenesis of hippocampal neuronal in vitro. First, the primary culture of hippocampal neurons from neonatal rats was established, and the purity of the neuronal cells was verified by immunofluorescent staining. To evaluate the effect of tramadol on neuronal cell viability MTT assay was carried out. The western blot analysis technique was performed for the assessment of apoptosis and synaptogenesis markers. Results show that chronic exposure to tramadol reduces cell viability of neuronal cells and naloxone reverses this effect. Also, the level of caspase‐3 significantly increased in tramadol‐exposed hippocampal neurons. Moreover, tramadol downregulates protein levels of synaptophysin and stathmin as synaptogenesis markers. Interestingly, the effects of tramadol were abrogated by naloxone treatment. These findings suggest that tramadol can induce neurotoxicity in hippocampal neuronal cells, and this effect was partly mediated through opioid receptors.
Colonic gallstone ileus treated by a transanal ileus tube followed by spontaneous gallstone dislodgement: A case report
Authors: Takagi Tadataka , Kinoshita Shoichi , Kawaguchi Chihiro , Kojima Kuniyuki , Ueno Hirotsugu , Nishimura Naoki , Shimozato Naotaka , Shirai Yasuyo , Noguchi Ryuichi , Ohyama Takao 
Published: July 2022

AbstractA 71‐year‐old obese woman was referred to our hospital with lower left abdominal pain. Computed tomography showed a 46 mm elliptic calcification lodged in the sigmoid‐descending colon junction (SDJ), which had been detected 5 years prior but was not within the gall bladder at presentation. Therefore, we diagnosed colonic gallstone ileus with obstructive colitis caused by a gallstone. Colonoscopy revealed a smooth gallstone impacted at the sigmoid‐descending colon junction, which was not fixed and could be pushed proximally with the endoscope. Dislodgement of the stone was unsuccessful with both a large polypectomy snare and a retrieval basket. Considering the high risk of surgery, we chose a non‐surgical treatment strategy for obstructive colitis. Accordingly, a transanal ileus tube was placed to drain the proximal portion of the gallstone. The drainage of the colon by the ileus tube was satisfactory; the proximal colon was decompressed, ameliorating the obstructive colitis. Five days after tube placement, a colonoscopy revealed spontaneous passage of the gallstone into the rectum where it was finally removed. Cholecystocolonic fistula formation was confirmed by magnetic resonance imaging. We decided to surgically close the cholecystocolonic fistula to prevent future retrograde biliary infections. The surgery used a surgical stapler and was successful, with an uneventful postoperative course. Since radical surgical treatment of colonic gallstones and cholecystoenteric fistulas has a risk of postoperative morbidity and mortality, this case illustrates the importance of thoroughly considering nonsurgical interventions and surgeries for the safe treatment of colonic gallstone ileus.
Gene expression analysis of the Xenopus laevis early limb bud proximodistal axis
Authors: Hudson Daniel T. , Bromell Jessica S. , Day Robert C. , McInnes Tyler , Ward Joanna M. , Beck Caroline W. 
Published: July 2022

AbstractBackgroundLimb buds develop as bilateral outgrowths of the lateral plate mesoderm and are patterned along three axes. Current models of proximal to distal patterning of early amniote limb buds suggest that two signals, a distal organizing signal from the apical epithelial ridge (AER, Fgfs) and an opposing proximal (retinoic acid [RA]) act early on pattern this axis.ResultsTranscriptional analysis of stage 51 Xenopus laevis hindlimb buds sectioned along the proximal‐distal axis showed that the distal region is distinct from the rest of the limb. Expression of capn8.3, a novel calpain, was located in cells immediately flanking the AER. The Wnt antagonist Dkk1 was AER‐specific in Xenopus limbs. Two transcription factors, sall1 and zic5, were expressed in distal mesenchyme. Zic5 has no described association with limb development. We also describe expression of two proximal genes, gata5 and tnn, not previously associated with limb development. Differentially expressed genes were associated with Fgf, Wnt, and RA signaling as well as differential cell adhesion and proliferation.ConclusionsWe identify new candidate genes for early proximodistal limb patterning. Our analysis of RA‐regulated genes supports a role for transient RA gradients in early limb bud in proximal‐to‐distal patterning in this anamniote model organism.
Tipping the balance: The role of seed density, abiotic filters, and priority effects in seed‐based wetland restoration
Authors: Tarsa Emily E. , Holdaway Bailey M. , Kettenring Karin M. 
Published: July 2022

AbstractSowing native seeds is a common approach to reintroduce native plants to degraded systems. However, this method is often overlooked in wetland restoration despite the immense global loss of diverse native wetland vegetation. Developing guiding principles for seed‐based wetland restoration is critical to maximize native plant recovery, particularly in previously invaded wetlands. Doing so requires a comprehensive understanding of how restoration manipulations, and their interactions, influence wetland plant community assembly. With a focus on the invader Phragmites australis, we established a series of mesocosm experiments to assess how native sowing density, invader propagule pressure, abiotic filters (water and nutrients), and native sowing timing (i.e., priority effects) interact to influence plant community cover and biomass in wetland habitats. Increasing the density of native seeds yielded higher native cover and biomass, but P. australis suppression with increasing sowing densities was minimal. Rather, community outcomes were largely driven by invader propagule pressure: P. australis densities of ≤500 seeds/m2 maintained high native cover and biomass. Low‐water conditions increased the susceptibility of P. australis to dominance by native competitors. Early sowing of native seeds showed a large and significant benefit to native cover and biomass, regardless of native sowing density, suggesting that priority effects can be an effective restoration manipulation to enhance native plant establishment. Given the urgent wetland restoration need combined with the limited studies on seed‐based wetland restoration, these findings provide guidance on restoration manipulations that are grounded in ecological theory to improve seed‐based wetland restoration outcomes.
Comparative assessment of satellite‐ and drone‐based vegetation indices to predict arthropod biomass in shrub‐steppes
Authors: Traba J. , Gómez‐Catasús J. , Barrero A. , Bustillo‐de la Rosa D. , Zurdo J. , Hervás I. , Pérez‐Granados C. , García de la Morena E. L. , Santamaría A. , Reverter M. 
Published: July 2022

AbstractArthropod biomass is a key element in ecosystem functionality and a basic food item for many species. It must be estimated through traditional costly field sampling, normally at just a few sampling points. Arthropod biomass and plant productivity should be narrowly related because a large majority of arthropods are herbivorous, and others depend on these. Quantifying plant productivity with satellite or aerial vehicle imagery is an easy and fast procedure already tested and implemented in agriculture and field ecology. However, the capability of satellite or aerial vehicle imagery for quantifying arthropod biomass and its relationship with plant productivity has been scarcely addressed. Here, we used unmanned aerial vehicle (UAV) and satellite Sentinel‐2 (S2) imagery to establish a relationship between plant productivity and arthropod biomass estimated through ground‐truth field sampling in shrub steppes. We UAV‐sampled seven plots of 47.6–72.3 ha at a 4‐cm pixel resolution, subsequently downscaling spatial resolution to 50 cm resolution. In parallel, we used S2 imagery from the same and other dates and locations at 10‐m spatial resolution. We related several vegetation indices (VIs) with arthropod biomass (epigeous, coprophagous, and four functional consumer groups: predatory, detritivore, phytophagous, and diverse) estimated at 41–48 sampling stations for UAV flying plots and in 67–79 sampling stations for S2. VIs derived from UAV were consistently and positively related to all arthropod biomass groups. Three out of seven and six out of seven S2‐derived VIs were positively related to epigeous and coprophagous arthropod biomass, respectively. The blue normalized difference VI (BNDVI) and enhanced normalized difference VI (ENDVI) showed consistent and positive relationships with arthropod biomass, regardless of the arthropod group or spatial resolution. Our results showed that UAV and S2‐VI imagery data may be viable and cost‐efficient alternatives for quantifying arthropod biomass at large scales in shrub steppes. The relationship between VI and arthropod biomass is probably habitat‐dependent, so future research should address this relationship and include several habitats to validate VIs as proxies of arthropod biomass.
Identifying eating disorders in adolescents and adults with overweight or obesity: A systematic review of screening questionnaires
Authors: House Eve T. , Lister Natalie B. , Seidler Anna L. , Li Haozhen , Ong Wee Yee , McMaster Caitlin M. , Paxton Susan J. , Jebeile Hiba 
Published: July 2022

AbstractObjectiveThis review aimed to examine the validity of self‐report screening questionnaires for identifying eating disorder (ED) risk in adults and adolescents with overweight/obesity.MethodFive databases were searched from inception to September 2020 for studies assessing validation of self‐report ED screening questionnaires against diagnostic interviews in adolescents and adults with overweight/obesity. The review was registered with PROSPERO (https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=220013).ResultsTwenty‐seven papers examining 15 questionnaires were included. Most studies validated questionnaires for adults (22 of 27 studies), and most questionnaires (12 of 15) screened for binge eating or binge‐eating disorder (BED). The Eating Disorder Examination Questionnaire (sensitivity = .16–.88, specificity = .62–1.0) and Questionnaire on Eating and Weight Patterns (sensitivity = .07–1.0, specificity = .0–1.0) were most frequently validated (six studies each). Five studies of three questionnaires were in adolescents, with the Adolescent Binge‐Eating Disorder Questionnaire having highest sensitivity (1.0) but lower specificity (.27). Questionnaires designed to screen for BED generally had higher diagnostic accuracy than those screening for EDs in general.DiscussionQuestionnaires have been well validated to identify BED in adults with overweight/obesity. Validated screening tools to identify other EDs in adults and any ED in adolescents with overweight/obesity are lacking. Thus, clinical assessment should inform the identification of patients with co‐morbid EDs and overweight/obesity.Public SignificanceIndividuals with overweight/obesity are at increased risk of EDs. This review highlights literature gaps regarding screening for ED risk in this vulnerable group. This work presents possibilities for improving care of individuals with overweight/obesity by reinventing ED screening tools to be better suited to diverse populations.