bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonstructural protein 1 (nsp1) widespread RNA decay phenotype varies among Coronaviruses
Authors: Bermudez Yahaira , Miles Jacob , Muller Mandy 
Published: April 2022

AbstractExtensive remodeling of the host gene expression environment by coronaviruses nsp1 proteins is a well-documented and conserved piece of the coronavirus-host takeover battle. However, whether and how the underlying mechanism of regulation or the transcriptional target landscape differ amongst coronaviruses remains mostly uncharacterized. In this study we use comparative transcriptomics to investigate the diversity of transcriptional targets between four different coronavirus nsp1 proteins (from MERS, SARS1, SARS2 and 229E). In parallel, we performed Affinity Purification followed by Mass-Spectrometry to identify common and divergent interactors between these different nsp1. For all four nsp1 tested, we detected widespread RNA destabilization, confirming that both α- and β-Coronavirus nsp1 broadly affect the host transcriptome. Surprisingly, we observed that even closely related nsp1 showed little similarities in the clustering of genes targeted. Additionally, we show that the RNA targeted by nsp1 from the α-CoV 229E partially overlapped with MERS nsp1 targets. Given MERS nsp1 preferential targeting of nuclear transcripts, these results may indicate that these nsp1 proteins share a similar targeting mechanism. Finally, we show that the interactome of these nsp1 proteins differ widely. Intriguingly, our data indicate that the 229E nsp1, which is the smallest of the nsp1 proteins tested here, interacts with the most host proteins, while MERS nsp1 only engaged with a few host proteins. Collectively, our work highlights that while nsp1 is a rather well-conserved protein with conserved functions across different coronaviruses, its precise effects on the host cell is virus specific.SignificanceCoronaviruses extensively co-opt their host gene expression machinery in order to quicky benefit from the host resources. The viral protein nsp1 plays a major role in this takeover as nsp1 is known to induce a widespread shutdown of the host gene expression, both at the RNA and the translational level. Previous work characterized the molecular basis for nsp1-mediated host shutdown. However, this was mostly conducted in the context of β-coronaviruses and in particular SARS-CoV1, CoV2 and MERS due to the important public health burden that these viruses represent. Here instead, we explored the impact of nsp1 on the host using a comparative approach, defining the influence of 4 nsp1 protein from α- and β-coronaviruses. We delineated the impact of these 4 nsp1 on the host transcriptome and mapped their interactome. We revealed that host target range and interactomes vary widely among different nsp1, suggesting a viral-specific targeting. Understanding how these differences shape infection will be important to better inform antiviral drug development.
Monoclonal antibody 7H2.2 binds the C-terminus of the cancer-oocyte antigen SAS1B through the hydrophilic face of a conserved amphipathic helix corresponding to one of only two regions predicted to be ordered
Authors: Legg Max S. G. , Gagnon Susannah M. L. , Powell Cameron J. , Boulanger Martin J. , Li Andra J. J. , Evans Stephen V. 
Published: April 2022

The structure of the antigen-binding fragment (Fab) of mouse monoclonal antibody 7H2.2 in complex with a 15-residue fragment from the metalloproteinase sperm acrosomal SLLP1 binding protein (SAS1B), which is a molecular and cellular candidate for both cancer therapy and female contraception, has been determined at 2.75 Å resolution by single-crystal X-ray diffraction. Although the crystallization conditions contained the final 148 C-terminal residues of SAS1B, the Fab was observed to crystallize in complex with a 15-residue fragment corresponding to one of only two elements of secondary structure that are predicted to be ordered within the C-terminal region of SAS1B. The antigen forms an amphipathic α-helix that binds the 7H2.2 combining site via hydrophilic residues in an epitope that spans the length of the antigen α-helix, with only two CH–π interactions observed along the edge of the interface between the antibody and antigen. Interestingly, the paratope contains two residues mutated away from the germline (YL32F and YH58R), as well as a ProH96-ThrH97-AspH98-AspH99 insertion within heavy chain CDR3. The intact 7H2.2 antibody exhibits high affinity for the SAS1B antigen, with 1:1 binding and nanomolar affinity for both the SAS1B C-terminal construct used for crystallization (3.38 ± 0.59 nM) and a 15-amino-acid synthetic peptide construct corresponding to the helical antigen observed within the crystal structure (1.60 ± 0.31 nM). The SAS1B–antibody structure provides the first structural insight into any portion of the subdomain architecture of the C-terminal region of the novel cancer-oocyte tumor surface neoantigen SAS1B and provides a basis for the targeted use of SAS1B.
The influence of price and availability on university millennials’ organic food product purchase intention
Authors: Carrión Bósquez Nelson Geovany , Arias-Bolzmann Leopoldo Gabriel , Martínez Quiroz Ana Katherine 
Published: April 2022

PurposeThis study aims to provide one of the first research works that, using the constructs of the theory of planned behaviour (TPB), analysed the levels of purchase intention of organic products.Design/methodology/approachThe study was quantitative with a correlational scope and a cross-sectional design. The study population consisted of 566 university millennials from Ecuador. The results were processed through the exploratory factor analysis (EFA), the confirmatory factor analysis (CFA) and the structural equation modelling (SEM).FindingsThe subjective attitudes and norms influence intentions to buy organic products. However, price mediated by perceived behavioural control reduces purchase intentions, while product availability does not.Originality/valueThis study is one of the first research works, based on the constructs of the TPB which was carried out to know if the attitudes and subjective norms of Ecuadorian university millennials, are related to their purchase intentions for organic products.
Blockchain technology in construction organizations: risk assessment using trapezoidal fuzzy ordinal priority approach
Authors: Sadeghi Mahsa , Mahmoudi Amin , Deng Xiaopeng 
Published: April 2022

PurposeIn the digital transformation era, the construction industry is not immune to unintended consequences and disruptions of distributed ledger technologies like blockchain. At the micro-level, construction organizations need an in-depth understanding of blockchain risks to take proactive strategies for being on the safe side. This study seeks to answer “What are the risks associated with blockchain technology from the firm-level perspective? And how can this disruptive technology overshadow the business objectives and impact organizational criteria?”Design/methodology/approachThe current research proposes a novel model for risk assessment based on the trapezoidal fuzzy ordinal priority approach (OPA-F) in the multi-criteria decision-making (MCDM) context. The proposed model handles uncertainties of experts' judgment around three primary parameters: the importance of organizational criteria, the impact of blockchain risks on criteria and the probability of risk occurrence.FindingsThe case study shows that organizational “communication and information” is exposed to the most blockchain risk. On the contrary, blockchain has less to do with an organization's “corporate social responsibility.” Furthermore, effective blockchain risk management can bring about cost efficiency, quality and improved customer experience for this case study. In the end, the authors develop a conceptual blockchain risk management framework based on findings.Research limitations/implicationsThis study will broaden researchers' horizons regarding “blockchain in construction context” and “blockchain risk management.”Practical implicationsFurthermore, executives looking for blockchain-based solutions can benefit from research findings and lessons learned from this case study before decision-making. Lastly, the risk assessment model based on trapezoidal OPA-F can be used both for research purposes and industrial decision problems.Originality/valueTo the best of the authors’ knowledge, it is for the first time that the OPA-F is employed in a risk assessment model. Also, the original OPA-F is extended to trapezoidal OPA-F using trapezoidal fuzzy numbers, and it is the first attempt to evaluate blockchain risks facing construction organizations and develop a blockchain risk management framework accordingly.
An analysis of the linkages among fiscal vulnerability, financial stress and macroeconomic policies: an econometric study
Authors: Chandia Khurram Ejaz , Iqbal Muhammad Badar , Bahadur Waseem 
Published: April 2022

PurposeThis study aims to analyze the imbalances in the public finance structure of Pakistan’s economy and highlight the need for comprehensive reforms. Specifically, it aims to contribute to the empirical literature by analyzing the relationship between fiscal vulnerability, financial stress and macroeconomic policies in Pakistan’s economy between 1971 and 2020.Design/methodology/approachThe study develops an index of fiscal vulnerability, an index of financial stress and an index of macroeconomic policies. The fiscal vulnerability index is based on the patterns of fiscal indicators resulting from past trends of the selected variables in Pakistan’s economy. The financial stress in Pakistan is caused from the financial disorders that are acknowledged in the composite index, which is based on variables with the potential to indicate periods of stress stemming from the foreign exchange market, the securities market and the monetary policy components. The macroeconomic policies index is developed to analyze the mechanism through which fiscal vulnerability and financial stress have influenced macroeconomic policies in Pakistan. The causal association between fiscal vulnerability, financial stress and macroeconomic policies is analyzed using the auto-regressive distributive lags approach.FindingsThere exists a long-run relationship between the three indices, and a bi-directional causality between fiscal vulnerability and macroeconomic policies.Originality/valueThis study contributes to the development of a fiscal monitoring mechanism, which has the basic purpose of analyzing the refinancing risk of public liabilities. Moreover, it focuses on fiscal vulnerability from a macroeconomic perspective. The study tries to develop a framework to assess fiscal vulnerability in light of “The Risk Octagon” theory, which focuses on three risk components: fiscal variables, macroeconomic-disruption-associated shocks and non-fiscal country-specific variables. The initial contribution of this work to the literature is to develop a framework (a fiscal vulnerability index, financial stress index and macroeconomic policies index) for effective and result-oriented macro-fiscal surveillance. Moreover, empirical literature emphasized and advised developing countries to develop their own capacity mechanisms to assess their fiscal vulnerability in light of the IMF guidelines regarding vulnerability assessments. This study thus attempts to fulfill the said gap identified in literature.
Development a novel hydro-vibration technology for the formation of hats from fabrics
Authors: Kushevskiy Nikolay , Merezhko Nina , Osyka Viktor , Pavlenko Volodimir , Didyk Alla , Dombrovska Olena , Kalinsky Eugen , Kryvoruchko Myroslav , Chepok Roman , Shkvorets Olena 
Published: April 2022

PurposeThe purpose of the work is development a novel of hydro-vibration technology for the formation of hats from fabrics, which will expand the functionalities of application of various fabrics.Design/methodology/approachThe work is based on a novel technology of forming hats from different fabrics with the use of liquid-active working environment (LAWE). This hydro-vibration technology is characterized by high efficiency, productivity, manufacturability and potential opportunities when compared to existing technologies. This allows expanding its range of applications and increase functionality.FindingsIn the article, hydro-vibration technology is developed for the formation of hats from fabrics. As a result of the experiment, regression dependences of the shape stability coefficient on the formation factors having a close correlation were obtained. The performed optimization allowed determining the optimal values of technological parameters of the vibroforming process from fabrics: LAWE pressure 0.26 MPa, vibration frequency LAWE 4.3 Hz, forming time 74 s.Practical implicationsThe use of developed hydro-vibration technology has great practical significance in the textile industry. This technology increases labor productivity and reduces the cost of production of hats due to its high efficiency. Increased efficiency is provided by the use of special equipment, methods and optimal parameters of the hats formation. With sufficient refinement, the developed technology can be applied to other technological processes.Originality/valueOriginality of the work is using liquid-actin working environment at vibroforming of heads of headdresses from fabrics. It is determined that the use of LAWE is effective in the formation of hats. To ensure maximum plasticization of textile fibers in the fabric of the part and increase the force field, it is developed a novel hydro-vibration technology of forming the heads of hats from fabrics.
Innovative design and delivery of the business capstone course
Authors: Fleming Robert S. , Kowalsky Michelle 
Published: April 2022

PurposeThis article discusses the experiences of a university's business school in enhancing the preparation of undergraduates through the innovative design and delivery of Business Policy, the capstone course taken by all business students.Design/methodology/approachThe case discusses the proactive approaches taken to explicitly align efforts between thinking and doing, for both faculty course designers and student participants alike.FindingsTen strategic areas of innovation and improvement are identified and discussed as areas for individual course alignment as well as the impetus for college and university business program development and delivery.Practical implicationsThese innovations can be replicated by other institutions to enhance graduates' career preparation, pursue greater consistency with their organization's mission, develop external stakeholder engagement, and enhance internal collaboration with colleagues within the business school and across the university.Originality/valueThe unique value of this innovative and multifaceted approach was recognized by AACSB International, the elite accrediting agency for business schools, in several articles and at a conference on undergraduate program innovation.
How to successfully compete in modern export fruit markets? Lessons from a leading export producer in Argentina
Authors: Stubrin Lilia Inés , Marin Anabel , Yeyati Preiss Lara , Palacín Roitbarg Rocío 
Published: April 2022

PurposeThe purpose of this paper is to expand the understanding of the type of strategies that can be successful for firms located in the South to get integrated and compete in modern export fruit markets.Design/methodology/approachTo achieve the research purpose of the paper the authors carry out an in-depth case study. They analyze the export strategy of Patagonian Fruits Trade, an Argentinean leading exporter of apple, pear and kiwi.FindingsResults revealed that Patagonian Fruits Trade developed a strategy focused on supplying decommoditization to compete in modern fruit export markets. A key aspect of the firms' business model relies on its capability to meet the demand of high-income markets by providing conventional, organic and biodynamic club varieties. However, the sustainability of the strategy heavily relies on the firm's capability to fund club varieties' licenses and on the firm's ability to negotiate with clients and suppliers.Research limitations/implicationsAdopting a case study method limits the generalization of results. However, it provides new insights into the type of export strategies that can be successful in modern fruit markets as well as its main limitations.Originality/valueResults of the study, based on original empirical evidence, shed light on key factors for the integration of Southern fruit producers into modern fruit markets.
Work–life balance and performance relations during COVID 19 outbreak: a case study among university academic staff
Authors: Demir Ahmet , Budur Taylan 
Published: April 2022

PurposeThe aim of the current study was to evaluate the effects of work–life balance (WLB) on the employee motivation (EM), job satisfaction (JS) and emloyee performance (EP) of academic staff at universities.Design/methodology/approachIn this regard, the authors collected 490 valid data from academic staff of major public and private universities in Kurdistan Region of Iraq. WLB was evaluated under three dimensions as work interference with personal life (WIPL), personal life interference with work (PLIW) and work personal life enhancement (WPLE).FindingsThe results of the analyses showed that WIPL and PLIW negatively and significantly affected EM but did not have any significant impact on JS. WPLE affected both JS and EM significantly and positively. Lastly, JS did not have significant impact on EP while EM did.Research limitations/implicationsAs the research was conducted in Kurdistan Region of Iraq, it cannot be generalized to other countries and cultures.Practical implicationsBesides, the study suggests theoretical and practical implication as it was a special study proposed during COVID 19 lock downs.Originality/valueThere are very limited or no research works which attempt to study the pros and cons of WLB during such a catastrophic time, the study is useful for the university administrators and researchers in this field.
Employee influencer management: evidence from state-owned enterprises in Indonesia
Authors: Arief N. Nurlaela , Gregory Anne , Pangestu Aria Bayu , Ramdlany Dany Muhammad Athory , Sanjaya I Made Ariya 
Published: April 2022

PurposeThe purpose of this study is to explore how Indonesian state-owned enterprises (SOEs) select and “manage” employee influencers in order to engage more effectively with younger generations.Design/methodology/approachThe study applies a mixed-methods approach. Quantitative data were obtained by analyzing Instagram content with samples for analysis being taken from company-related posts from June 2018 to June 2020. Qualitative data collection was by two focus group discussions (FGD). The FGDs comprised in total, 22 employee influencers representing the 11 sectors of SOEs in Indonesia.FindingsThe article examines how employee influencers engage with others; how they are chosen by their organizations; how they are managed and the support they need from their employers. It was discovered that a careful triangulation is required between employees as influencers, their followers and SOE communication hubs. A key factor is maintaining the authentic relationship between employee influencers and their followers. A conceptual model of employee influencer management for Indonesia is proposed.Research limitations/implicationsThe research provides useful insights for communication management, marketing, and human resources in developing and supporting the role of employee influencers.Practical implicationsThe research provides useful insights for communication management, marketing and human resources in developing and supporting the role of employee influencers. The suggested model is of practical utility for SOEs for managing employee influencers in Indonesia and provides valuable indicators for other countries.Originality/valueThe study of SOEs’ employee influencers has not been explored previously in the literature. This, combined with the Indonesian perspective, brings new insights to the field. Social media use is especially high in Indonesia, so it acts as a good exemplar for the field. It also builds on the growing literature about the importance of employees as influencer, especially in the social media space. The model also make a theoretical contribution.
Prototyping an IoT-based system for monitoring building indoor environment
Authors: Zhou Na , Chang-Richards Alice , Wang Kevin I-Kai , Dirks Kim Natasha 
Published: April 2022

Purpose
This study aims to develop an architectural prototype of a Cyber-Physical System (CPS), as well as lay a technological foundation for future smart housing with improved health and well-being outcomes for its occupants.

Design/methodology/approach
This study deploys smart sensors to monitor the key environmental parameters of a house. Using Internet of Things technology, a prototype of a CPS has been developed for capturing the environmental conditions over time. A case study involving a property in New Zealand was undertaken to validate the prototype.

Findings
The study proposes a monitoring platform, enabled by the CPS and smart sensing devices, that collects, shares, stores, analyses and visualises indoor environment data. The reliability and accuracy of the monitoring system were enhanced by comparing the activity of house occupants with sensor data.

Research limitations/implications
Due to limited time, the prototype was tested in one house for a period of one month. Air quality was not considered in this study. However, the work suggests that such an approach provides an effective solution for government organisations and housing agencies to collect information for the purpose of assessing building thermal performance.

Originality/value
This research proposes a new lens consisting of a home environment monitoring application with health and well-being implications. It could also be used to inform the future design of healthy homes and buildings, both in New Zealand and internationally.