bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biliary Tract Exploration After Laparoscopic Left-sided Hepatectomy: A Comparative Study of Left Hepatic Duct Orifice Versus Common Bile Duct Approach
Authors: Tu Shuju , Sun Gen , Fang Kang , Xin Wanpeng , Zha Ming , Wan Renhua , Li Yong , Xiao Weidong 
Published: August 2022

Background:
Laparoscopic left-sided hepatectomy (LLH) and additional biliary tract exploration are effective methods to treat left-sided hepatolithiasis (LSH) combined with extrahepatic bile duct stones. Although biliary tract exploration through common bile duct (CBD) incision has been widely accepted, the safety and effectiveness of the left hepatic duct (LHD) orifice approach after LLH is still in debate.

Methods:
One hundred and forty-four patients with LSH who underwent LLH and biliary tract exploration in our institution from April 2014 to September 2021 were enrolled in the retrospectively study. They were divided into 3 groups: LHD group (n=67), CBD/T-tube group (n=58), and CBD/PC group (n=19). Patients’ demographic characteristics, intraoperative, and postoperative outcomes were retrospectively analyzed.

Results:
LHD group exhibited a shorter operative time (202.8±42.2 vs. 232.7±47.5 min, P=0.000), time to first bowel movement (2.3±0.5 vs. 2.9±0.7 d, P=0.000) and postoperative hospital stay (7.5±2.1 vs. 9.8±5.2 d, P=0.001) compared with the CBD/T-tube group. The lithotomy time in the LHD group was significantly longer than that in the CBD/T-tube group (33.6±7.3 vs. 29.0±6.3 min, P=0.000) and CBD/PC group (33.6±7.3 vs. 28.7±3.7, P=0.006). Intraoperative blood loss, blood transfusion rate, initial stone clearance rate, and stone recurrence rate all had no significant differences between the 3 groups (all P>0.05). LHD group showed less rate of electrolyte imbalance than that of the CBD/T-tube group (3.0% vs. 19.0%, P=0.004) but it was equivalent to the CBD/PC group (P>0.05). The type of biliary tract exploration (odds ratio: 5.43, 95% confidence interval: 0.04-0.95, P=0.032) as independent predictors of electrolyte imbalance. No reoperation and mortality occurred in the 3 groups. The conversion rate was comparable among 3 groups (1.5% vs. 1.7% vs. 0, all P>0.05). No significant difference in stone recurrence rate was seen (1.5% vs. 3.4% vs. 0, all P>0.05).

Conclusion:
Biliary tract exploration through LHD orifice after LLH is a safe and effective treatment for selected patients with LSH, with an advantage over the T-tube drainage in the field of operative time, the incidence of electrolyte imbalance, recovery of gastrointestinal function, and postoperative hospital stay.
A Practically Modified Approach with Complete Posterior Mobilization for Three-port Sleeve Gastrectomy: Single-center Experience
Authors: Pan Hsin-Mei , Liao Guo-Shiou , Lin Chien-Hua , Feng Ting-Yuan , Hsu Fang-Chin , Chang Po-Chih , Hsu Kuo-Feng 
Published: August 2022

Background:
Although the procedure of laparoscopic sleeve gastrectomy (LSG) has been standardized either in conventional lateral to medial or medial to lateral approach, surgeons occasionally face the challenge of poor visualization of the His angle and difficulty in complete posterior mobilization in limited surgical field. This study aimed to introduce our novel details of modified approach to address these issues.

Methods:
One hundred patients with obesity underwent modified approach- three-port laparoscopic sleeve gastrectomy. Herein, we demonstrated our method to ease the procedure of gastric fundus mobilization with extensive posterior mobilization (shown in video, Supplemental Digital Content 1, http://links.lww.com/SLE/A336). The demographic characteristics and perioperative data were reviewed.

Results:
There was no case of conversion to open surgery. The mean operative time, volume of blood loss, and hospital stay were 72.5±22.7 minutes, 11.6±10.5 mL, and 4.3±2.1 days, respectively. One postoperative leakage was observed and it was successfully treated with metallic covered stent. The percentage of total weight loss at 6 months and 1 year were 20.3±8.4 and 29.8±9.2, respectively.

Conclusions:
Our experience showed that the modified technique is feasible and may help surgeons to accomplish a complete posterior mobilization, and better address the poor visualization of the the His angle-site owing to the interposition of floating omentum or bulging part of the stomach.
Registry of the International Society of Uterus Transplantation: First Report
Authors: Brännström Mats , Tullius Stefan G. , Brucker Sara , Dahm-Kähler Pernilla , Flyckt Rebecca , Kisu Iori , Andraus Wellington , Wei Li , Carmona Francisco , Ayoubi Jean-Marc , Scollo Paolo , Weyers Steven , Fronek Jiri 
Published: August 2022

Background.
Uterus transplantation (UTx) is a novel type of transplantation to treat infertility in women with an absent or nonfunctioning uterus. The International Society of Uterus Transplantation (ISUTx) has developed a registry to monitor worldwide UTx activities while serving as a repository for specific research questions.

Methods.
The web-based registry has separate data fields for donor, recipient, surgeries, immunosuppression, rejections, pregnancies with live birth(s), and transplant hysterectomies. Data are prospectively registered.

Results.
A total of 45 UTx procedures have been registered; the majority (78%) of those procedures were live donor (LD) transplants. Median age of the LDs, deceased donors, and recipients were 50 y (range 32–62), 38.5 y (19–57), and 29 y (22–38), respectively. The duration of LD surgery was approximately twice as long as the recipient surgery. Postoperative complications of any Clavien-Dindo grade were registered in 20% of LDs and 24% of recipients. Rejection episodes were more frequent (33%) early after transplantation (months 1–5) compared with later time points (months 6–10; 21%). Healthy neonates were delivered by 16 recipients, with 3 women giving birth twice. The total live birth rate per embryo transfer was 35.8%. Median length of pregnancy was 35 gestational weeks. Twelve uteri were removed without childbirth, with 9 transplant hysterectomies occurring during the initial 7 mo post-UTx.

Conclusions.
A mandatory registry is critical to determine quality and process improvement for any novel transplantation. This registry provides a detailed analysis of 45 UTx procedures performed worldwide with a thorough analysis of outcomes and complications.
Nasogastric tube care and flushing in a Portuguese general medicine ward: a best practice implementation project
Authors: Fernandes António M. , Henriques Pedro , Lourenço Ana R. , Mateus Fernando , Santos Joana , Marques Daniela , Apóstolo João L.A. , Cardoso Daniela F.B. , Klugar Miloslav , Klugarová Jitka 
Published: August 2022

Abstract

Objectives:
To implement evidence-based practice related to care and flushing of nasogastric tubes inserted for feeding, in a general medicine ward.

Introduction:
Nasogastric tube feeding is a widely used nutritional support form. Although performed by qualified professionals, it is not a harm-free intervention. Progression to the trachea during initial insertion, aspiration of gastric contents, improper position for patient feeding, and/or a blocked tube are examples of problems that can occur.

Methods:
The project used JBI's methodological approach of the Clinical Evidence System and Getting Research into Practice audit and feedback tool. A baseline audit focused on nursing procedures related to the maintenance of nasogastric tube feeding, based on 14 criteria informed by the JBI evidence summaries, was performed for a month. Education sessions and other engagement strategies and resources were used to increase nasogastric tube maintenance. This best-practice implementation was conducted in Portugal, between January 2020 and April 2021, in a 33-bed general medicine ward with a staff of 30 registered nurses.

Results:
Different samples, for each audited procedure, were obtained from a universe of 25 nurses and 14 episodes of patients with nasogastric tube in baseline and 10 in follow-up audit. At baseline, compliance with the criteria ranged from 0 to 88%. Ten of the 14 criteria were below 50%. The follow-up audit showed significant improvement in all compliance criteria (ranging from 44.4 to 100%). Criteria 1 and 2, which were aimed at preventing adverse consequences related to wrong placement or displacement of the nasogastric tube, showed a high postimplementation compliance level (89.3 and 90%).

Conclusion:
With an integrated plan incorporating interventions, strategies, and resources, tailored for this context, we improved nurses’ knowledge of nasogastric tube maintenance and achieved significantly increased compliance with nasogastric tube care best-practice. We suggest maintaining current strategies, and reinforcing clinical supervision and regular assessment.
Optimization of Klebsiella pneumoniae Biofilm Preservation for Scanning Electron Microscopy Reveals Impact of Capsule on Biofilm Formation.
Authors: Fleeman Renee M , Mikesh Michelle , Davies Bryan W 
Published: August 2022

Klebsiella pneumoniae biofilm formation is associated with chronic and relapsing infections. Scanning electron microscopy (SEM) is a powerful tool for characterizing biofilm structure and studying their formation. Reliable visualization of biofilm structure requires careful sample preservation, otherwise there may be loss of non-covalent interactions that are susceptible to damage during the dehydration and washing preparation steps. However, no standard procedure has been adopted in literature to fix K. pneumoniae biofilm for scanning electron microscopy studies. This lack of standardization makes it challenging to compare results between studies and determine if optimal fixation methods have been achieved to preserve native structures. To advance this critical area of study, we describe a scanning electron microscopy fixation method optimized for K. pneumoniae biofilm preservation. Our study reveals the importance of optimizing the biofilm preparation steps before scanning electron microscopy to observe differences in biofilm architecture between strains. Using our optimized fixation, we show that although species that overproduce capsular extracellular polysaccharides produced more robust biofilms, K. pneumoniae can form a developed biofilm in the absence of capsular polysaccharides.
Does phasic dopamine release cause policy updates?
Authors: Carter Francis , Cossette Marie-Pierre , Trujillo-Pisanty Ivan , Pallikaras Vasilios , Breton Yannick-André , Conover Kent , Caplan Jill , Solis Pavel , Voisard Jacques , Yaksich Alexandra , Shizgal Peter 
Published: August 2022

AbstractPhasic dopamine activity is believed to both encode reward-prediction errors (RPEs) and to cause the adaptations that these errors engender. If so, a rat working for optogenetic stimulation of dopamine neurons will repeatedly update its policy and/or action values, thus iteratively increasing its work rate. Here, we challenge this view by demonstrating stable, non-maximal work rates in the face of repeated optogenetic stimulation of midbrain dopamine neurons. Furthermore, we show that rats learn to discriminate between world states distinguished only by their history of dopamine activation. Comparison of these results to reinforcement learning simulations suggests that the induced dopamine transients acted more as rewards than RPEs. However, pursuit of dopaminergic stimulation drifted upwards over a time scale of days and weeks, despite its stability within trials. To reconcile the results with prior findings, we consider multiple roles for dopamine signaling.
Early activation of cellular stress and death pathways caused by cytoplasmic TDP-43 in the rNLS8 mouse model of ALS/FTD
Authors: Luan Wei , Wright Amanda L. , Brown-Wright Heledd , Le Sheng , Gil Rebecca San , Martin Lidia Madrid San , Ling Karen , Jafar-Nejad Paymaan , Rigo Frank , Walker Adam K. 
Published: August 2022

AbstractTAR DNA binding protein 43 (TDP-43) pathology is a key feature of over 95% of amyotrophic lateral sclerosis (ALS) and nearly half of frontotemporal dementia (FTD) cases. The pathogenic mechanisms of TDP-43 dysfunction are poorly understood, however activation of cell stress pathways may contribute to pathogenesis. We therefore sought to identify which cell stress components are critical for driving disease onset and neurodegeneration in ALS/FTD. We studied the rNLS8 transgenic mouse model, which expresses human TDP-43 with a genetically-ablated nuclear localisation sequence within neurons of the brain and spinal cord resulting in cytoplasmic TDP-43 pathology and progressive motor dysfunction. Amongst numerous cell stress-related biological pathways profiled using qPCR arrays, several critical ISR effectors, including CCAAT/enhancer-binding homologous protein (Chop/Ddit3) and activating transcription factor 4 (Atf4), were upregulated in the cortex of rNLS8 mice prior to disease onset. This was accompanied by early up-regulation of anti-apoptotic gene Bcl2 and diverse pro-apoptotic genes including BH3-interacting domain death agonist (Bid). However, pro-apoptotic signalling predominated after onset of motor phenotypes. Notably, pro-apoptotic caspase-3 protein was elevated in the cortex of rNLS8 mice at later disease stages, suggesting that downstream activation of apoptosis drives neurodegeneration following failure of early protective responses. Unexpectedly, suppression of Chop in the brain and spinal cord using antisense oligonucleotide-mediated silencing had no effect on overall TDP-43 pathology or disease phenotypes in rNLS8 mice. Cytoplasmic TDP-43 accumulation therefore causes very early activation of ISR and both anti-and pro-apoptotic signalling that switches to predominant pro-apoptotic activation later in disease. These findings suggest that precise temporal modulation of cell stress and death pathways may be beneficial to protect against neurodegeneration in ALS and FTD.Key pointsISR genes Atf4 and Chop, anti-apoptotic Bcl2 and pro-apoptotic gene Bid, Bim, Noxa were upregulated in the cortex of rNLS8 mice prior to disease onsetKnockdown of Chop had limited effects on TDP-43 pathology and did not alter motor deficits in rNLS8 miceBoth anti-and pro-apoptotic genes are upregulated prior to disease onset, and switches to activation of pro-apoptotic signalling at later disease stagesCaspase-3 activation likely drives neurodegeneration in the cortex of rNLS8 mice
Right anterior insula effective connectivity impairs intrinsic BOLD fluctuations in dorsal attention network in adolescents and young adults with borderline personality symptoms
Authors: Hall Nathan T. , Hallquist Michael N. 
Published: August 2022

AbstractBackgroundBorderline Personality Disorder (BPD) symptoms often emerge in adolescence. However, little is known about the functional organization of intrinsic brain networks in young people with BPD symptoms.MethodsIn this study we collected resting-state fMRI data in a sample of adolescents and young adults with (nBPD = 40) and without BPD (nHC= 42) symptoms. Using a detailed cortico-limbic parcellation coupled with graph theoretical analyses, we tested for group and age-related differences in regional functional and effective connectivity (FC, EC) and amplitude of low frequency fluctuations (ALFF). We conducted a series of analyses that progressed from global network properties to focal tests of EC amongst nodes in Salience (SN) and Dorsal Attention Networks (DAN).ResultsAt the regional level, regularized regression analyses revealed a broad pattern of hyper-connectivity and heightened ALFF in R dorsal anterior insula (daIns), in addition to hypoconnectivity in R temporal-parietal junction (TPJ) and decreased ALFF in multiple DAN regions. Furthermore, analyses of EC amongst daIns, TPJ, and DAN revealed that in BPD participants daIns exerts a heightened influence on TPJ and DAN regions. Finally, multivariate mediation models indicated that lower DANALFF was differentially predicted by EC from TPJ and daIns.ConclusionsOur findings provide converging evidence that heightened EC from daIns impairs network-wide ALFF in DAN both directly and indirectly via impaired TPJ functioning. We interpret this pattern of findings in line with an “attentional hijacking” account of borderline personality.
Dynamics of Pulsatile Activities of Arcuate Kisspeptin Neurons in Aging Female Mice
Authors: Goto Teppei , Hagihara Mitsue , Miyamichi Kazunari 
Published: August 2022

AbstractReproductive senescence is broadly observed across mammalian females, including humans, eventually leading to a loss of fertility. The pulsatile secretion of gonadotropin-releasing hormone (GnRH), which is essential for gonad function, is primarily controlled by kisspeptin neurons in the hypothalamic arcuate nucleus (ARCkiss), the pulse generator of GnRH. The pulsatility of GnRH release, as assessed by the amount of circulating gonadotropin, is markedly reduced in aged animals, suggesting that the malfunctions of ARCkissmay be responsible for reproductive aging and menopause-related disorders. However, the activity dynamics of ARCkissduring the natural transition to reproductive senescence remain unclear. Herein, we introduce chronicin vivoCa2+imaging of ARCkissin female mice by fiber photometry to monitor the synchronous episodes of ARCkiss(SEskiss), a known hallmark of GnRH pulse generator activity, from the fully reproductive to acyclic phase over 1 year. During the reproductive phase, we find that not only the frequency, but also the intensities and waveforms of individual SEskiss, vary depending on the stage of the estrus cycle. During the transition to reproductive senescence, the integrity of SEskisspatterns, including the frequency and waveforms, remains mostly unchanged, whereas the intensities tend to decline. These data illuminate the temporal dynamics of ARCkissactivities in aging female mice. More generally, our findings demonstrate the utility of fiber-photometry-based chronic imaging of neuroendocrine regulators in the brain to characterize aging-associated malfunction.Significance StatementThe transition to reproductive senescence significantly impacts the quality of life of women, but the underlying neural mechanisms remain poorly characterized. Goto and Miyamichi establish long-term (from the reproductive to acyclic phase) chronic imaging of the central pacemaker activities of reproductive functions by fiber photometry in female mice. In particular, they focus on kisspeptin neurons in the arcuate nucleus of the hypothalamus. Their data reveal that during the transition to reproductive senescence, the pulsatile activities of kisspeptin neurons show unexpected robustness in terms of frequency, but a tendency for the intensity to decline. Their findings exhibit the power of direct chronic visualization of hormonal regulators in the brain, which is generally applicable to facilitate studies of aging-associated malfunctions in neuroendocrine systems.
Ori-Finder 2022: A Comprehensive Web Server for Prediction and Analysis of Bacterial Replication Origins
Authors: Dong Meijing , Luo Hao , Gao Feng 
Published: August 2022

AbstractThe replication of DNA is a complex biological process that is essential for life. Bacterial DNA replication is initiated at genomic loci referred to as replication origins (oriCs). Integrating the Z-curve method, DnaA box distribution, and comparative genomic analysis, we developed a web server to predict bacterial oriCs in 2008 called Ori-Finder, which contributes to clarify the characteristics of bacterial oriCs. The oriCs of hundreds of sequenced bacterial genomes have been annotated in their genome reports using Ori-Finder and the predicted results have been deposited in DoriC, a manually curated database of oriCs. This has facilitated large-scale data mining of functional elements in oriCs and strand-biased analysis. Here, we describe Ori-Finder 2022 with updated prediction framework, interactive visualization module, new analysis module, and user-friendly interface. More species-specific indicator genes and functional elements of oriCs are integrated into the updated framework, which has also been redesigned to predict oriCs in draft genomes. The interactive visualization module displays more genomic information related to oriCs and their functional elements. The analysis module includes regulatory protein annotation, repeat sequence discovery, homologous oriC search, and strand-biased analyses. The redesigned interface provides additional customization options for oriC prediction. Ori-Finder 2022 is freely available at http://tubic.tju.edu.cn/Ori-Finder2022 and https://tubic.org/Ori-Finder2022.
Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction
Authors: Spens Amy E. , Sutliff Nicholas A. , Bennett Sean R. , Campbell Amy E. , Tapscott Stephen J. 
Published: August 2022

ABSTRACTDUX4 activates the first wave of zygotic gene expression in the early embryo. Mis-expression of DUX4 in skeletal muscle causes facioscapulohumeral dystrophy (FSHD), whereas expression in cancers suppresses IFNγ-induction of MHC Class I and contributes to immune evasion. We show that the DUX4 protein interacts with STAT1 and broadly suppresses expression of IFNγ stimulated genes by decreasing bound STAT1 and Pol-II recruitment. Transcriptional suppression of ISGs requires conserved (L)LxxL(L) motifs in the carboxyterminal region of DUX4 and phosphorylation of STAT1 Y701 enhances interaction with DUX4. Consistent with these findings, expression of endogenous DUX4 in FSHD muscle cells and the CIC-DUX4 fusion containing the DUX4 CTD in a sarcoma cell line inhibit IFNγ-induction of ISGs. Mouse Dux similarly interacted with STAT1 and suppressed IFNγ induction of ISGs. These findings identify an evolved role of the DUXC family in modulating immune signaling pathways with implications for development, cancers, and FSHD.
From proteins to nanoparticles: domain-agnostic predictions of nanoscale interactions
Authors: Saldinger Jacob , Raymond Matt , Elvati Paolo , Violi Angela 
Published: August 2022

AbstractThe accurate and rapid prediction of generic nanoscale interactions is a challenging problem with broad applications. Much of biology functions at the nanoscale, and our ability to manipulate materials and engage biological machinery in a purposeful manner requires knowledge of nano-bio interfaces. While several protein-protein interaction models are available, they leverage protein-specific information, limiting their abstraction to other structures. Here, we present NeCLAS, a general, and rapid machine learning pipeline that predicts the location of nanoscale interactions, providing human-intelligible predictions. Two key aspects distinguish NeCLAS: coarsegrained representations, and the use of environmental features to encode the chemical neighborhood. We showcase NeCLAS with challenges for protein-protein, protein-nanoparticle and nanoparticle-nanoparticle systems, demonstrating that NeCLAS replicates computationally- and experimentally-observed interactions. NeCLAS outperforms current nanoscale prediction models and it shows cross-domain validity. We anticipate that our framework will contribute to both basic research and rapid prototyping and design of diverse nanostructures in nanobiotechnology.