bluuebunny/crossref_metadata_2025_split huggingface dataset:
Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms
Authors: Van Ngoc Huynh , Quyen Than Linh , Vinayaka Aaydha Chidambara , Bang Dang Duong , Wolff Anders 
Published: August 2022

The COVID-19 pandemic emphasized the importance of rapid, portable, and on-site testing technologies necessary for resource-limited settings for effective testing and screening to reduce spreading of the infection. Realizing this, we developed a fluorescence-based point-of-care (fPOC) detection system with real-time reverse transcriptase loop-mediated isothermal amplification for rapid and quantitative detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. The system is built based on the Arduino platform compatible with commercially available open-source hardware–software and off-the-shelf electronic components. The fPOC system comprises of three main components: 1) an instrument with integrated heaters, 2) optical detection components, and 3) an injection-molded polymeric cartridge. The system was tested and experimentally proved to be able to use for fast detection of the SARS-CoV-2 virus in real-time in less than 30 min. Preliminary results of testing the performance of the fPOC revealed that the fPOC could detect the SARS-CoV-2 virus at a limit of detection (LOD50%) at two to three copies/microliter (15.36 copies/reaction), which was comparable to reactions run on a standard commercial thermocycler. The performance of the fPOC was evaluated with 12 SARS-CoV-2 clinical throat swab samples that included seven positive and five negative samples, as confirmed by reverse transcription–polymerase chain reaction. The fPOC showed 100% agreement with the commercial thermocycler. This simple design of the fPOC system demonstrates the potential to greatly enhance the practical applicability to develop a totally integrated point-of-care system for rapid on-site screening of the SARS-CoV-2 virus in the management of the pandemic.
Selection of Staphylococcus aureus in a murine nasopharyngeal colonization model
Authors: Salgado Breno A. B. , Waters Elaine M. , Moran Josephine C. , Kadioglu Aras , Horsburgh Malcolm J. 
Published: August 2022

Staphylococcus aureus nasal colonization is a risk factor for infection. A large proportion of the population are identified as potential S. aureus carriers yet we only partially understand the repertoire of genetic factors that promote long-term nasal colonization. Here we present a murine model of nasopharyngeal colonization that requires a low S. aureus inoculum and is amenable to experimental evolution approaches. We used this model to experimentally evolve S. aureus using successive passages in the nasopharynx to identify those genetic loci under selection. After 3 cycles of colonization, mutations were identified in mannitol, sorbitol, arginine, nitrite and lactate metabolism genes promoting key pathways in nasal colonization. Stress responses were identified as being under selective pressure, with mutations in DNA repair genes including dnaJ and recF and key stress response genes clpL, rpoB and ahpF. Peptidoglycan synthesis pathway genes also revealed mutations indicating potential selection for alteration of the cell surface. The murine model used here is versatile to question colonization, persistence and evolution studies.We studied the human pathogen Staphylococcus aureus in our search to determine factors that contribute to its ability to live in the human nose and throat. The anterior nares and nasopharynx are considered primary habitats but we do not understand how the pathogen adapts as it moves from one person to the next. We first determined sustained survival of the pathogen over multiple days in the nasopharynx that might act as a good model for human persistence due to the low numbers of bacteria needed for it to establish. By using successive rounds of colonization of the nasopharynx across different mice we revealed that multiple genetic changes in the S. aureus occurred. These changes were found in genes associated with the cell surface and metabolism and might indicate adaptation to the niche. One gene showed an accumulation of multiple mutations supporting a key contribution in adaptation but the role of the protein it encodes is not yet known. The contribution of these genes and genetic changes are unclear but indicate an area for future research to better understand how this common human pathogen is so successful at human colonization and survival.
The association of long-term blood pressure variability with hemodialysis access thrombosis
Authors: Hsieh Mu-Yang , Cheng Chi-Hung , Chen Chiu-Hui , Liao Min-Tsun , Lin Chih-Ching , Yang Ten-Fang , Chuang Shao-Yuan , Wu Chih-Cheng 
Published: August 2022

BackgroundBlood pressure variability (BPV) is an important risk factor for cardiovascular events in hemodialysis patients. We sought to determine the impact of BPV on hemodialysis access thrombosis.MethodsWe enrolled 1,011 prevalent hemodialysis patients from 12 hemodialysis centers since January 2018 and followed them until December 2020. Predialysis blood pressure (BP) was assessed at 12-week intervals. The coefficient of variation derived from 36 consecutive BP measurements was used as the metric for variability. The primary outcome was incident hemodialysis access thrombosis. Linear regression models were used to assess factors associated with BPV at baseline. Kaplan-Meier curves of the time until vascular access events were drawn and log-rank tests were calculated. Cox proportional hazards models were performed to assess the association of BPV with incident vascular access events.ResultsThe average coefficient of variance for systolic BPV was 10.9%. BPV was associated with age, body mass index, mean BP, diabetes, coronary and peripheral artery disease, history of access dysfunction, graft access, intradialytic hypotension, and use of antihypertensive medications. There were 194 access thrombosis events and 451 access stenosis events during a median follow-up period of 30 months. After adjustment of potential confounding factors, BPV was associated with increased risk of access thrombosis [hazard ratio = 1.27, 95% confidence interval (CI), 1.18–1.44, per 1 standard deviation increase in BPV]. The patients in the highest BPV quartile had 2.45 times the risk of thrombosis (CI, 1.62–3.70). The association was independent of average BP, intradialytic hypotension, and comorbidities. Similar trends of association were found in the subgroups analyzed. Comparative analysis using a time-varying variable model and different metrics of BPV showed consistent results.ConclusionOur findings underscored the impact of BP fluctuation on vascular access thrombosis.
Ideal cardiovascular health status and risk of cardiovascular disease and all-cause mortality: over a decade of follow-up in the Tehran lipid and glucose study
Authors: Hadaegh Farzad , Hosseinpour-Niazi Somayeh , Deravi Niloofar , Hasheminia Mitra , Moslehi Nazanin , Toreyhi Hossein , Azizi Fereidoun 
Published: August 2022

ObjectiveTo quantify the association between ideal cardiovascular health (CVH) metrics and incident cardiovascular disease (CVD) including different subtypes [coronary heart disease (CHD), stroke, and sudden death], and all-cause mortality in an Iranian population.MethodsThe study population included 6,388 participants (2,726 men) aged 48.0 ± 12.4 years free of CVD at baseline. We utilized the American Heart Association’s 2020 impact target criteria of ideal, intermediate, and poor CVH. The multivariate Cox proportional Hazard model, adjusted for age, sex, educational level, marital status, and family history of CVD, was applied to estimate the hazard ratio (HR) of outcomes per one additional metric of ideal CVH metrics. Furthermore, the risk was also calculated for ideal and intermediate categories considering poor category as a reference.ResultsDuring the median follow-up of 11.26 years, 692 CVD, 589 CHD, 130 stroke, 111 sudden death, and 519 all-cause mortality events were reported. All of the individual ideal CVH metrics were independent predictors except intermediate physical activity level for CVD, BMI &lt; 25 kg/m2, and intermediate physical activity for all-cause mortality. Each additional metrics of ideal CVH decreased the risk by 31 (0.69, 0.65–0.73) for CVD, 32 (0.68, 0.64–0.73) for CHD, 31 (0.69, 0.60–0.80) for stroke, 25 (0.75, 0.64–0.88) for sudden death, and 13% (0.87, 0.81–0.93) for all-cause mortality events. Moreover, intermediate and ideal categories of CVH metrics were associated with lower risk for different CVD outcomes, i.e., 44 (0.56, 0.48–0.65) and 76% (0.24, 0.17–0.35) for CVD; 43 (0.57, 0.47–0.67) and 75% (0.25, 0.16–0.37) for CHD, 58 (0.42, 0.29–0.61) and 86% (0.14, 0.04–0.44) for stroke; 56 (0.44, 0.29–0.66) and 55% (0.45, 0.21–0.99) for sudden death; and 25 (0.75, 0.62–0.90) and 46% (0.54, 0.37–0.80) for all-cause mortality events, respectively. We also assessed the impact of changes in ideal CVH status from phase III to phase IV (2008–2011) on CVD events among 5,666 participants. Accordingly, compared to those remaining in the poor category, all of the changes in ideal CVH categories showed a lower risk for CVD events.ConclusionAmong the Iranian population, meeting higher ideal CVH metrics is associated with a lower risk of different CVD events and mortality outcomes.
Relationship between serum growth differentiation factor 15, fibroblast growth factor-23 and risk of atrial fibrillation: A systematic review and meta-analysis
Authors: Tan Ziqi , Song Tiangang , Huang Shanshan , Liu Menglu , Ma Jianyong , Zhang Jing , Yu Peng , Liu Xiao 
Published: August 2022

Background and objectiveGrowth differentiation factor-15 (GDF-15) and fibroblast growth factor-23 (FGF-23) are considered predictors of the incidence of cardiovascular diseases. The present meta-analysis aimed to elucidate the associations between GDF-15 and FGF-23 in the risk of atrial fibrillation (AF).MethodsAn electronic search was conducted in the Cochrane Library, PubMed, and Embase databases from inception until February 27, 2021. The study protocol was registered in the PROSPERO database (CRD42020182226).ResultsIn total, 15 studies that enrolled 36,017 participants were included. Both serum FGF-23 and GDF-15 were elevated in patients with AF. Analysis of categorical variables showed higher serum FGF-23 levels were associated with an increased risk of AF [relative risk (RR) = 1.28, 95% confidence interval (CI): 1.05–1.56]. In contrast, this association was not found with GDF-15 (RR = 0.91, 95% CI: 0.20–4.04). In dose-response analysis, a linear positive association was noted between serum FGF-23 levels and the risk of AF (P nonlinear = 0.9507), with a RR elevation of 7% for every 20 pg/ml increase in the serum FGF-23 levels (95% CI: 1.02–1.13). No remarkable relationship was found between serum GDF-15 levels and the risk of AF, and the overall RR for the association between a 100 ng/L increment in GDF-15 levels and AF was 1.01 (95% CI: 0.998–1.02).ConclusionOur study showed a positive linear correlation between serum FGF-23 levels and the risk of AF. However, no significant association was found between GDF-15 and the risk of AF. Further studies are warranted to clarify whether serum FGF-23 levels may be considered in predicting the risk of AF.Systematic Review Registration:http:www.york.ac.uk/inst/crd, identifier CRD42020182226.
An improved seven-level switched-capacitor-based neutral-point clamped inverter
Authors: Kakar Saifullah , Arif M. Saad Bin , Ayob Shahrin Md. , Nordin Norjulia B. M. , Mekhilef Saad , Seyedmahmoudian Mehdi , Stojcevski Alex 
Published: August 2022

Multilevel inverters (MLIs) have seen significant growth in a variety of medium- to high-power industrial applications and have become a mature and viable solution for renewable energy systems over the past decade. The major concerns of MLIs are the high component count and the buck-type feature of the ac output voltage when used for grid-connected renewable-energy-based sources such as solar photovoltaic (PV) panels. As another option, a switched-capacitor (SC)-based inverter possessing the feature of boost ability is a prime solution to meet the requirements of ac power applications. Integrating an SC network with a neutral-point clamped (NPC) structure makes leakage current alleviation viable for transformerless PV grid-connected systems. In this article, an improved SCMLI based on the NPC structure is proposed to reduce the number of active switches and the number of switches in the charging loop, significantly reducing the power loss. It can generate seven voltage levels with voltage boosting and self-balancing of capacitors within a single-stage power conversion. The working principle of the proposed topology, circuit description, and control technique are presented. Further, the proposed inverter was compared with other recent SC topologies to show its superiority. In the end, a laboratory prototype was developed and tested for the seven-level module under steady-state and dynamic conditions to validate the practical viability of the proposed configuration. The proposed inverter’s maximum efficiency was 97.5% at 1.2 kW rated power.
Remote bednet use monitoring to describe patterns of use and exposure to female Anopheles mosquitoes in an Ugandan cohort
Authors: Krezanoski Paul J. , Rek John , Musiime Alex , Otto Geoffrey , Kyagamba Patrick , Rwatooro Jackson Asiimwe , Walters Kelly , Romanel Alina , Arinaitwe Emmanuel , Nankabirwa Joaniter I. , Drakeley Chris J. , Kamya Moses , Dorsey Grant 
Published: August 2022

BackgroundLong lasting insecticide-treated bednets (LLINs) are the most widely used tool for preventing malaria. There has been a plateau in progress in the highest burden African countries since 2015, leading to questions about the effectiveness of LLINs. In this study, remote LLIN use monitors were deployed in a cohort in Eastern Uganda to explore how LLIN use interacts with mosquito exposure.MethodsThe SmartNet study included 20 households from May to October 2019. SmartNet devices recorded, every 15 min, whether an LLIN was unfurled or folded up. Unannounced visits were used to assess SmartNet accuracy. Risk factors associated with poor LLIN use were assessed using generalized linear equations. Female Anopheles exposure was estimated by combining hourly probabilities of exposure from human landing catches and measures of density from biweekly CDC light traps in participants rooms. Mosquito exposure averted by LLINs was quantified using SmartNet measurements and age-related differences were estimated using generalized linear equations, adjusting for relevant covariates and household clustering.Results96 individuals contributed 5,640 SmartNet observation nights. In 126 unannounced visits, SmartNet had an area under the curve of 0.869 in classifying whether the LLIN was up or down. The rate of non-use was 13.5% of nights (95% CI: 12.6–14.3%). Compared to children under 5, non-use was 1.8 times higher (95% CI: 1.6–2.1; p &lt; 0.001) in children 5–15 years and 2.6 times higher (95% CI: 2.2–3.1; p &lt; 0.001) in participants aged 15– &lt;30years. There was no difference between children under 5 years and adults &gt; 30 years. LLIN use averted 50.3% of female Anopheles mosquito exposure (95% CI: 40.0–60.0%), with decreasing point estimates of efficacy across age groups: from 61.7% (95% CI: 42.6–80.7%) in children under 5 years to 48.0% (95% CI: 29.1–66.8%) in adults over 30.ConclusionsObjective monitors are accurate and can feasibly be deployed to obtain data about LLIN use. LLINs provided protection from only 50% of female Anopheles mosquito exposure in this cohort and protection was dependent upon age. In assessing the role of LLINs in malaria prevention it is crucial to consider the dynamics between mosquito exposure and LLIN use behaviors.
Effect of laser scanning speed on microstructure and mechanical properties of SLM porous Ti-5Al-5V-5Mo-3Cr-1Fe alloy
Authors: Yuan Wei , Zhao Xiaoli , Li Shujun , Zhu Yue 
Published: August 2022

In this study, porous Ti-55531(Fe) was fabricated by selective laser melting (SLM) with different laser scanning speeds. The microstructures, surface morphology, inner defects, porosity, microhardness, and compressive behaviors were studied. The variation of lattice constant and hardness were analyzed. The results show that all the specimens have a density of ∼1 g/cm3, and a Vicker’s hardness with a range of 280–320 Hv0.1. The porosity of the SLM-produced materials is greater than the designed value (77%) and increases from 77.33% to 82.33% with the increase of laser scanning speed from 500 mm/s to 1,500 mm/s. Continuous irregular columnar dendrites, a large number of gas-induced defects with small size between 20 and 60 μm and a deep molten pool form in the specimens fabricated with a laser scanning speed less than 1,000 mm/s. Some defects, elongated voids and interrupted columnar dendrites are identified in the specimens fabricated with the laser scanning speed more than 1,000 mm/s caused by the insufficient input energy. All specimens with different laser scanning speeds show the single ß phase patterns. The compressive strength of the specimens with the laser scanning speed of 500 mm/s is maintained at 32 MPa and the compressive strength decreases with the increase of laser scanning speed. The specimens with a scanning speed of 500 mm/s present the best mechanical properties and surface quality.
Examining distractor qualities of pediatrics subject tests from a national assessment
Authors: Pan Qianqian , Jiang Zhehan 
Published: August 2022

BackgroundAnalyzing distractor qualities of a pediatrics subject test in a national-level examination is vital in developing high-quality items for the discipline. Yet traditional approaches focus on key answers only and therefore are less informative. The number of distractors can also be parsimonized to improve the item development.Materials and methodsFrom a pediatrics subject test at the national level, raw responses of 44,332 examines to nineteen multiple-choice questions were analyzed, such that the distractor qualities were evaluated via traditional and advanced methods such as canonical correlation index. Additionally, a simulation study was conducted to investigate the impact of eliminating distractor numbers on reliability.ResultsThe traditional item analysis showed that most items had acceptable psychometric properties, and two items were flagged for low item difficulty and discrimination. Distractor analysis showed that about one-third of items had poorly functioning distractors based on relatively a low choice frequency (&lt;5%) and a small effect size of distractor discrimination. The simulation study also confirmed that shrinking distractor numbers to 4 was viable.ConclusionsIntegrating multiple methods, especially the advanced ones, provides comprehensive evaluations of the item quality. Simulations can help re-consider the decision to set distractor numbers for cost-effectiveness. These proposed methods can improve further development of the pediatrics subject test.
Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats
Authors: Nascimento Mariana Moura , Bernardo Desiree Rita Denelle , de Bragança Ana Carolina , Massola Shimizu Maria Heloisa , Seguro Antonio Carlos , Volpini Rildo Aparecido , Canale Daniele 
Published: August 2022

BackgroundTenofovir disoproxil fumarate (TDF), a widely prescribed component in antiretroviral regimens, has been associated with nephrotoxicity. Nebivolol is a third generation selective β-1 adrenergic receptor blocker and may protect renal structure and function through the suppression of oxidative stress and enhancement of nitric oxide (NO) synthesis. We aimed to investigate whether nebivolol could be an effective therapeutic strategy to mitigate tenofovir-induced nephrotoxicity.MethodsWe allocated Wistar rats to four groups: control (C), received a standard diet for 30 days; NBV, received a standard diet for 30 days added with nebivolol (100 mg/kg food) in the last 15 days; TDF, received a standard diet added with tenofovir (300 mg/kg food) for 30 days; and TDF+NBV, received a standard diet added with tenofovir for 30 days and nebivolol in the last 15 days.ResultsLong-term exposure to tenofovir led to impaired renal function, induced hypertension, endothelial dysfunction and oxidative stress. Nebivolol treatment partially recovered glomerular filtration rate, improved renal injury, normalized blood pressure and attenuated renal vasoconstriction. Administration of nebivolol contributed to reductions in asymmetric dimethylarginine (ADMA) levels as well as increases in endothelial nitric oxide sintase (eNOS) accompanied by renin-angiotensin-aldosterone system downregulation and decreases in macrophage and T-cells infiltrate. Furthermore, nebivolol was responsible for the maintenance of the adequate balance of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) levels and it was associated with reductions in NADPH oxidase (NOX) subunits.ConclusionNebivolol holds multifaceted actions that promote an advantageous option to slow the progression of kidney injury in tenofovir-induced nephrotoxicity.
Volatile organic compound profiling as a potential biomarker in irritable bowel syndrome: A feasibility study
Authors: Van Malderen Kathleen , Hanning Nikita , Lambrechts Helen , Haverhals Tine , Van Marcke Silke , Ceuleers Hannah , De Man Joris G. , De Winter Benedicte Y. , Lamote Kevin , De Schepper Heiko U. 
Published: August 2022

BackgroundIrritable bowel syndrome (IBS) is a chronic gastrointestinal disorder for which no diagnostic tools are currently available. Patients are diagnosed using the Rome IV criteria and subtyped into a diarrhea, constipation, or mixed phenotype based on their dominant stool pattern. A recent development in the biomarker area is the analysis of volatile organic compounds (VOCs). The aim of this study was to evaluate the potential of VOCs as diagnostic and phenotypic biomarkers for IBS in breath and fecal samples.Materials and methodsBreath and fecal samples from IBS patients and healthy asymptomatic controls (HC) were analyzed with multicapillary column/ion mobility spectrometry (MCC/IMS) and classification models were created based upon VOCs and clinical characteristics.DiscussionIrritable bowel syndrome patients were differentiated from HC by means of volatile profiling in both breath and fecal samples with area under the curve (AUCs) of respectively 0.62 and 0.80. Patient subtypes could also be differentiated from each other with AUCs ranging between 0.65 and 0.78. Furthermore, VOC models could differentiate IBS patients based on clinical characteristics like psychological comorbidities and microbiota-influencing therapies.ConclusionThis study is the first to demonstrate the use of VOC profiling with the help of MCC/IMS to differentiate IBS patients. Furthermore, the importance of clinical characteristics beside the dominant stool pattern in the differentiation of IBS patients was emphasized.