bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA CRLM1 inhibits apoptosis and promotes metastasis through transcriptional regulation cooperated with hnRNPK in colorectal cancer
Authors: Wang Zhe , Chen Jianfang , Sun Fengjun , Zhao Xiang , Dong Yan , Yu Songtao , Li Jianjun , Liang Houjie 
Published: July 2022

Abstract
Background
Colorectal liver metastases (CRLM) continue to have a low survival rate. The number of CRLM regulators and clinical indicators remains limited. Long non-coding RNAs (lncRNAs) are a new master regulator of cell invasion and metastasis. However, the function and regulation mechanism of lncRNAs in colorectal cancer (CRC) metastasis are yet unknown.

Methods
To screen and identify CRLM-related lncRNAs, public transcriptome data were used. Gain and loss of function experiments were carried out to investigate the biological activities of lncRNA CRLM1 in vitro and in vivo. RNA sequencing (RNA-seq), chromatin isolation by RNA purification (ChIRP), immunofluorescence (IF), quantitative real-time PCR (qRT-PCR), western blotting, and rescue experiments were performed to explore the molecular mechanism of CRLM1. Moreover, identified the proteins, DNAs, and RNAs that interact with CRLM1.

Results
The investigation of lncRNA expression dynamics in CRLM, primary CRC, and normal tissues in this work resulted in identifying a series of lncRNAs associated with metastasis, including CRLM1. CRLM1 inhibited apoptosis of CRC cells and promoted liver metastasis in Balb/C nude mice. CRLM1 was weakly associated with the chromatin regions of genes involved in cell adhesion and DNA damage, and this association was bidirectionally correlated with CRLM1-regulated pro-metastatic gene expression. CRLM1 physically interacts with the hnRNPK protein and promotes its nuclear localization. CRLM1 effectively enhances hnRNPK promoter occupancy and co-regulates the expression of a panel of metastatic genes.

Conclusions
The finding of the clinically significant lncRNA CRLM1 in promoting metastasis and regulating gene expression suggests a potential biomarker and target for CRLM therapy.
Paper 4: a review of reporting and disseminating approaches for rapid reviews in health policy and systems research
Authors: Kelly Shannon E. , McGowan Jessie , Barnhardt Kim , Straus Sharon E. 
Published: July 2022

Abstract
Background
Transparent reporting of rapid reviews enables appropriate use of research findings and dissemination strategies can strengthen uptake and impact for the targeted knowledge users, including policy-makers and health system managers. The aim of this literature review was to understand reporting and dissemination approaches for rapid reviews and provide an overview in the context of health policy and systems research.

Methods
A literature review and descriptive summary of the reporting and disseminating approaches for rapid reviews was conducted, focusing on available guidance and methods, considerations for engagement with knowledge users, and optimizing dissemination. MEDLINE, PubMed, Google scholar, as well as relevant websites and reference lists were searched from January 2017 to March 2021 to identify the relevant literature with no language restrictions. Content was abstracted and charted.

Results
The literature review found limited guidance specific to rapid reviews. Building on the barriers and facilitators to systematic review use, we provide practical recommendations on different approaches and methods for reporting and disseminating expedited knowledge synthesis considering the needs of health policy and systems knowledge users. Reporting should balance comprehensive accounting of the research process and findings with what is “good enough” or sufficient to meet the requirements of the knowledge users, while considering the time and resources available to conduct a review. Typical approaches may be used when planning the dissemination of rapid review findings; such as peer-reviewed publications or symposia and clear and ongoing engagement with knowledge users in crafting the messages is essential so they are appropriately tailored to the target audience. Consideration should be given to providing different products for different audiences. Dissemination measures and bibliometrics are also useful to gauge impact and reach.

Conclusions
Limited guidance specific to the reporting and dissemination of rapid reviews is available. Although approaches to expedited synthesis for health policy and systems research vary, considerations for the reporting and dissemination of findings are pertinent to all.
General decay for weak viscoelastic equation of Kirchhoff type containing Balakrishnan–Taylor damping with nonlinear delay and acoustic boundary conditions
Authors: Yoon Min , Lee Mi Jin , Kang Jum-Ran 
Published: July 2022

AbstractIn this paper, we consider the general decay of solutions for the weak viscoelastic equation of Kirchhoff type containing Balakrishnan–Taylor damping with nonlinear delay and acoustic boundary conditions. By using suitable energy and Lyapunov functionals, we prove the general decay for the energy, which depends on the behavior of bothσandk.
Current insight into the regulation of PD-L1 in cancer
Authors: Liu Zhuandi , Yu Xibao , Xu Ling , Li Yangqiu , Zeng Chengwu 
Published: July 2022

AbstractThe molecular mechanisms underlying cancer immune escape are a core topic in cancer immunology research. Cancer cells can escape T cell-mediated cellular cytotoxicity by exploiting the inhibitory programmed cell-death protein 1 (PD-1)/programmed cell death ligand 1 (PD-L1, CD274) immune checkpoint. Studying the PD-L1 regulatory pattern of tumor cells will help elucidate the molecular mechanisms of tumor immune evasion and improve cancer treatment. Recent studies have found that tumor cells regulate PD-L1 at the transcriptional, post-transcriptional, and post-translational levels and influence the anti-tumor immune response by regulating PD-L1. In this review, we focus on the regulation of PD-L1 in cancer cells and summarize the underlying mechanisms.
Noninvasive fetal genotyping of single nucleotide variants and linkage analysis for prenatal diagnosis of monogenic disorders
Authors: Wu Wenman , Zhou Xuanyou , Jiang Zhengwen , Zhang Dazhi , Yu Feng , Zhang Lanlan , Wang Xuefeng , Chen Songchang , Xu Chenming 
Published: July 2022

Abstract
Background
High-cost, time-consuming and complex processes of several current approaches limit the use of noninvasive prenatal diagnosis (NIPD) for monogenic disorders in clinical application. Thus, a more cost-effective and easily implementable approach is required.

Methods
We established a low-cost and convenient test to noninvasively deduce fetal genotypes of the mutation and single nucleotide polymorphisms (SNPs) loci by means of targeted amplification combined with deep sequencing of maternal genomic and plasma DNA. The sequential probability ratio test was performed to detect the allelic imbalance in maternal plasma. This method can be employed to directly examine familial pathogenic mutations in the fetal genome, as well as infer the inheritance of parental haplotypes through a group of selected SNPs linked to the pathogenic mutation.

Results
The fetal mutations in 17 families with different types of monogenic disorders including hemophilia A, von Willebrand disease type 3, Duchenne muscular dystrophy, hyper-IgM type 1, glutaric acidemia type I, Nagashima-type palmoplantar keratosis, and familial exudative vitreoretinopathy were identified in the study. The mutations included various forms: point mutations, gene inversion, deletions/insertions and duplication. The results of 12 families were verified by sequencing of amniotic fluid samples, the accuracy of the approach in fetal genotyping at the mutation and SNPs loci was 98.85% (172/174 loci), and the no-call rate was 28.98% (71/245 loci). The overall accuracy was 12/12 (100%). Moreover, the approach was successfully applied in plasma samples with a fetal fraction as low as 2.3%.

Conclusions
We have shown in this study that the approach is a cost-effective, less time consuming and accurate method for NIPD of monogenic disorders.
Impact of Ageing on Female Metabolic Flexibility: A Cross-Sectional Pilot Study in over-60 Active Women
Authors: Monferrer-Marín Jordi , Roldán Ainoa , Monteagudo Pablo , Chulvi-Medrano Iván , Blasco-Lafarga Cristina 
Published: July 2022

Abstract
Background
Ageing affects metabolic flexibility, although physical status could influence this relationship. This cross-sectional study aims to describe and analyse the metabolic flexibility/inflexibility in a group of active older women, together with the impact of ageing and physical status on their oxidation rates and maximal fat oxidation (MFO).

Methods
Fifteen volunteers (69.00 ± 6.97 years)—from 24 women—completed an incremental cycling test until the second ventilatory threshold. Intensity increased 10 W each 3 min 15 s, starting at 30 W. Gas exchange, heart rate, rate of perceived effort, pain scale and muscle power were registered, together with lactate. VO2 and VCO2 were considered for fat and carbohydrate oxidation (FATox and CHOox; Frayn’s equation) at intensities 60%, 80% and 100% from the peak power in the test (P100). Psychophysiological parameters were compared at MFO/FATmax and P100, together with the energy expenditure calculations around MFO (included FAT and CHO contributions), and the main correlation analyses, with and without P100 and VO2 as covariates.

Results
FATox was low at MFO (0.13; 95% CI [0.09–0.17] mg/min/kgFFM; 3.50; 95% CI [2.49–4.50] mg/min/kgFFM), with short oxidation-rate curves shifting down and leftward. CHOox and FATox were both low for reduced power with age (77.14 ± 18.58 W and 39.29 ± 9.17 W at P100 and MFO, respectively), all accompanied by a fall in energy expenditure (5.44 ± 2.58 kcal/min and 3.32 ± 1.55 kcal/min at P100 and MFO, respectively). Power appears as a determinant factor, given its strong and negative significant association with age (r = − 0.85, p < 0.005; R2 = 0.72) and moderate with MFO (r = − 0.54, p = 0.04; R2 = 0.29). In turn, energy expenditure shows a positive and moderate association with muscle power (r = 52, p = 0.04).

Conclusions
Despite the drop in substrates oxidation with age, physical status (i.e. larger muscular power and energy expenditure) suggests a key role in the preservation of metabolic health with ageing in active women.
A comprehensive in silico exploration of the impacts of missense variants on two different conformations of human pirin protein
Authors: Khan Auroni Semonti , Parvez Nahid , Ahsan Tamim , Shoily Sabrina Samad , Sajib Abu Ashfaqur 
Published: July 2022

Abstract
Background
Pirin, a member of the cupin superfamily, is an iron-binding non-heme protein. It acts as a coregulator of several transcription factors, especially the members of NFκB transcription factor family. Based on the redox state of its iron cofactor, it can assume two different conformations and thereby act as a redox sensor inside the nucleus. Previous studies suggested that pirin may be associated with cancer, inflammatory diseases as well as COVID-19 severities. Hence, it is important to explore the pathogenicity of its missense variants. In this study, we used a number of in silico tools to investigate the effects of missense variants of pirin on its structure, stability, metal cofactor binding affinity and interactions with partner proteins. In addition, we used protein dynamics simulation to elucidate the effects of selected variants on its dynamics. Furthermore, we calculated the frequencies of haplotypes containing pirin missense variants across five major super-populations (African, Admixed American, East Asian, European and South Asian).

Results
Among a total of 153 missense variants of pirin, 45 were uniformly predicted to be pathogenic. Of these, seven variants can be considered for further experimental studies. Variants R59P and L116P were predicted to significantly destabilize and damage pirin structure, substantially reduce its affinity to its binding partners and alter pirin residue fluctuation profile via changing the flexibility of several key residues. Additionally, variants R59Q, F78V, G98D, V151D and L220P were found to impact pirin structure and function in multiple ways. As no haplotype was identified to be harboring more than one missense variant, further interrogation of the individual effects of these seven missense variants is highly recommended.

Conclusions
Pirin is involved in the transcriptional regulation of several genes and can play an important role in inflammatory responses. The variants predicted to be pathogenic in this study may thus contribute to a better understanding of the underlying molecular mechanisms of various inflammatory diseases. Future studies should be focused on clarifying if any of these variants can be used as disease biomarkers.
Effects of repeated fire on Florida oak-saw palmetto scrub
Authors: Schmalzer Paul A. , Foster Tammy E. 
Published: July 2022

Abstract
Background
The dominant species of Florida oak-saw palmetto scrub sprout after burning from belowground rhizomes or fire-resistant aboveground buds with rapid reestablishment of cover. Responses to single fires are well documented; however, responses to repeated fires may differ. Fire return intervals, differences among sites, and species may influence responses. We used transect data from four sites on Kennedy Space Center/Merritt Island National Wildlife Refuge to test whether growth differed through repeated fires. Two sites burned five times in 36 years, one site burned five times in 25 years, and one burned four times in 18 years. We used linear mixed models that account for repeated measures to determine if the number of fires affected height, total cover ≥ 0.5 m and < 0.5 m, bare ground, and cover of the dominant oak (Quercus) ≥ 0.5 m and of saw palmetto (Serenoa repens) ≥ 0.5 m. We compared community composition through repeated fires using nonmetric multidimensional scaling ordination.

Results
Height, total cover ≥ 0.5 m, and cover of the dominant oak ≥ 0.5 m and of saw palmetto ≥ 0.5 m increased with time since burn; total cover < 0.5 m and bare ground decreased. A quadratic term in the growth model was significant except for total cover < 0.5 m. There were site differences for all variables except bare ground. The number of fires decreased height, total cover ≥ 0.5 m, and cover of the dominant oak ≥ 0.5 and increased total cover < 0.5 m and bare ground but had no effect on cover of Serenoa repens ≥ 0.5 m. Community changes after repeated fires were similar in nonmetric multidimensional ordinations with time since burn correlated to the first or second axis.

Conclusions
Scrub recovered from repeated fires at a range of intervals and seasons, but short return intervals reduced growth with responses differing among species.
Egyptian vowel second formant in isolation and in transition following high-frequency fricatives by NLFC hearing aid in children: how does the pattern compare to typically hearing peers?
Authors: Saleh Marwa Mahmoud , Mohammed Hassnaa Othman , El Danasoury Iman Sadek , Hassan Dalia Mohamed , Sarwat Sally A. , Elrefaie Dina A. 
Published: July 2022

AbstractBackgroundHigh-frequency consonants form an area of difficulty among children with hearing impairment. Many technological setups have emerged in order to improve their audibility, among them nonlinear frequency compression (NLFC). Therefore, the following study was carried out in order to evaluate change in production of high-frequency fricatives by hearing-impaired children utilizing NLFC hearing aids. This was through spectrographic analysis of F2 onset of transition elicited by fricatives in a prevocalic context, and results were compared to typically developing (TD) children. A change in mid-frequency sounds due to high-frequency compression was also tested through production of sustained vowels in isolation.ResultsA preliminary prospective case-control study involved 9 patients with NLFC hearing aids, evaluated at 1- and at 6-month post-fitting, without receiving speech therapy. Spectrographic analysis of F2 formant onset in [a] and [i] vowels when combined with [s] and [f] fricatives in a CV (consonant-vowel) pattern, presented auditorily and audio-visually, was observed and analyzed. One patient was excluded due to noncompliance in attendance. In the 8 patients who completed the study, very high-frequency level of F2 onset in CV was notable in second versus first evaluation in NLFC group, to the extent of surpassing the typical value in TD control group with [i] vowel. Contrastingly, F2 of sustained vowels in isolation took a trend of a lower value after 6 months of NLFC hearing aid use, as compared to the first evaluation. Consequently, the gap between sustained vowel F2 level in TD and NLFC groups widened.ConclusionMean high-frequency range of F2 onset of transition in CV patterns of voiceless fricatives [s] and [f] with [i] and [a] vowels 6 months after NLFC fitting reflects an enhancement in the frequency range production of these sounds. A shift toward a lower F2 frequency range in sustained mid-frequency vowels, on the other hand, remains to be carefully investigated as a sequel of nonlinear frequency compression in the Egyptian hearing-impaired children.
Quality of life outcomes in acoustic neuroma: systematic review (2000–2021)
Authors: Barker-Collo Suzanne , Miles Anna , Garrett Joanne 
Published: July 2022

Abstract
Background
The majority of acoustic neuroma (AN) outcome studies examine medical outcomes. An alternative is to examine how AN, its treatment, and complications impact patients’ quality of life (QOL). A systematic review for AN was conducted using MEDLINE, PUBMED, and EMBASE.
Manuscripts were limited to human participants, written in English, and published from 2000 to 2021. Of 426 unique citations, only 48 examined QOL outcomes. Risk of bias was assessed using Downs and Black’s Quality Assessment Index.

Results
Sixteen studies (33.3%) compared AN patients to normative/control data, 15 (31.25%) compared QOL outcomes between treatment groups, 8 (16.7%) examined changes in QOL over time within a treatment modality, 7 (14.6%) examined QOL in relation to a specific sample characteristic, and 2 (4.2%) used non-standard methods to describe patients experiences. QOL was worse post-surgery and/or radiotherapy compared with healthy controls and active surveillance and tied to symptoms experienced. Study quality was reasonable despite expectable limitations due to the nature of the population.

Conclusions
AN treatments, particularly surgical, may result in pain and nerve damage. Whilst not common, these can significantly impact QOL domains, including social and emotional wellbeing and social participation. Surgical teams should prepare patients for this possibility and ensure adequate community follow-up with specialist physical therapies, audiology, ophthalmology, and psychology to mitigate these effects should they occur.
Preservation techniques and their effect on nutritional values and microbial population of brewer’s spent grain: a review
Authors: Terefe Geberemariyam 
Published: July 2022

AbstractThe most prevalent by-product produced by the brewery factory is brewer's spent grain (BSG). A total of 70%, 10%, and 20% of the BSG produced are used for animal feed, biogas production, and landfills, respectively. Feeding wet brewery spent grain can avoid the cost of drying. Wet brewery spent grain is used as a replacement for forage in the diets of animals. The high moisture content and ease of deterioration of wet brewery leftover grain as a fresh feed are drawbacks (3–5 days). BSG is provided as a low-cost feed despite its greater perishability and microbiological instability. There are two significant challenges brought on by the BSG's higher moisture content (80%). First, transportation is expensive. Second, the abundance of proteins and polysaccharides in BSG promotes microbial development and deterioration. Therefore, these problems can be solved by utilizing various preservation methods, including drying (solar, freeze, and oven drying), freezing, ensiling (both alone and in combination with other animal feeds), and additives (Silo-King GPX preservatives, xylanase, carbohydrase (econase) and protease (alcalase), urea and lime, sodium formate, calcium propionate, formic and propionic acids, acetic acid, NaCl, NaOH, HCl, and H2SO4).
Empiric treatment of patients with sepsis and septic shock and place in therapy of cefiderocol: a systematic review and expert opinion statement
Authors: Cortegiani Andrea , Ingoglia Giulia , Ippolito Mariachiara , Girardis Massimo , Falcone Marco , Pea Federico , Pugliese Francesco , Stefani Stefania , Viale Pierluigi , Giarratano Antonino 
Published: July 2022

AbstractCarbapenem-resistant Gram-negative bacteria are frequent causes of sepsis and septic shock in intensive care unit (ICU) and thus considered a public health threat. Until now, the best available therapies consist of combinations of preexisting or new antibiotics with β-lactamase inhibitors (either new or preexisting). Several mechanisms of resistance, especially those mediated by metallo-β-lactamases (MBL), are responsible for the inefficacy of these treatments, leaving an unmet medical need. Intravenous cefiderocol has been recently approved by the American Food and Drug Administration (FDA) and European Medicines Agency (EMA) for the treatment of complicated urinary tract infections and nosocomial pneumonia due to Gram-negative, when limited therapeutical options are available. In addition, its ability to hijack bacterial iron uptake mechanisms makes cefiderocol stable against the whole Ambler β-lactamase inhibitors and increases the in vitro efficacy against Gram-negative pathogens (e.g., Enterobacterales spp., Pseudomonas aeruginosa, and Acinetobacter baumannii). Trials have already demonstrated their non-inferiority to comparators. In 2021, ESCMID guidelines released a conditional recommendation supporting the use of cefiderocol against metallo-β-lactamase-producing Enterobacterales and against Acinetobacter baumannii. This review provides the opinion of experts about the general management of empiric treatment of patients with sepsis and septic shock in the intensive care unit and detects the proper place in therapy of cefiderocol considering recent evidence sought through a systematic search.