bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC00240 knockdown inhibits nasopharyngeal carcinoma progress by targeting miR‐26a‐5p
Authors: Chen Xing , Wu Guixiang , Qing Jing , Li Chunlin , Chen Xudong , Shen Jian 
Published: May 2022

AbstractObjectiveThis study intended to explore the regulatory functions of LINC00240 on nasopharyngeal carcinoma (NPC).MethodsMiR‐26a‐5p inhibitor, mimic, and siLINC00240 were transfected into NPC cells. QRT‐PCR was employed to assess miR‐26a‐5p and LINC00240 expressions. The targeting relationship of LINC00240 and miR‐26a‐5p was analyzed through dual luciferase reporter and RNA immunoprecipitation assay. Cell counting kit‐8 assay, colony formation assay, flow cytometry assay, wound healing assay, Transwell assay and in vitro angiogenesis assay were adopted for the evaluation of the effects of LINC00240 or miR‐26a‐5p and LINC00240 on NPC cells regarding cell proliferation, apoptosis and cycle, migration, invasion, and angiogenesis. EZH2, cell cycle, and epithelial‐mesenchymal transition (EMT)‐related protein expression was tested through Western blot.ResultsLINC00240 had a high expression in NPC tissues and cell lines. Silenced LINC00240 significantly suppressed the 5‐8F and HK1 cell proliferation, invasion, migration, and angiogenesis, but raised cell apoptosis, and cells were blocked in G0/G1 phase. MiR‐26a‐5p was a target of LINC00240. MiR‐26a‐5p upregulation suppressed the NPC cell proliferation, migration, invasion, angiogenesis, N‐cadherin and EZH2 expression, while it elevated apoptosis and p21, p27 and E‐cadherin expressions, whereas miR‐26a‐5p downregulation performed conversely. LINC00240 knockdown partially offset the effects of miR‐26a‐5p downregulation on cell proliferation, migration, invasion, angiogenesis, apoptosis, and EZH2.ConclusionLINC00240 knockdown restrained cell proliferation, invasion, migration, and angiogenesis, while it advanced apoptosis via miR‐26a‐5p in NPC by EZH2 inhibition.
The role of ERNDIM diagnostic proficiency schemes in improving the quality of diagnostic testing for inherited metabolic diseases
Authors: Mathis Déborah , Croft Joanne , Chrastina Petr , Fowler Brian , Vianey‐Saban Christine , Ruijter George J. G. 
Published: May 2022

AbstractExternal quality assurance (EQA) is crucial to monitor and improve the quality of biochemical genetic testing. ERNDIM (www.erndim.org), established in 1994, aims at reliable and standardized procedures for diagnosis, treatment and monitoring of inherited metabolic disease (IMD) by providing EQA schemes and educational activities. Currently, ERNDIM provides 16 different EQA schemes including quantitative schemes for various metabolite groups, and interpretive schemes such as diagnostic proficiency testing (DPT). DPT schemes focus on the ability of laboratories to correctly identify and interpret abnormalities in authentic urine samples across a wide range of IMDs. In the DPT schemes, six samples each year are distributed together with clinical information. Laboratories choose and perform the tests needed to reach a diagnosis. Data were collected on 345 samples, distributed to up to 105 laboratories worldwide. Diagnostic proficiency (the % of total points possible for all participating laboratories within a scheme for analysis and interpretation) ranged widely: amino acid disorders (n = 20), range 33%–100%, mean 84%; organic acid disorders (n = 35), range 14%–100%, mean 84%; lysosomal storage disorders (n = 13), range 20%–97%, mean 73%; purine/pyrimidine disorders (n = 9), range 37%–100%, mean 70%; miscellaneous disorders (n = 8), range 17%–100%, mean 65%; no IMD, range 65%–95%, mean 85%. When a sample with the same disorder was distributed in a subsequent survey, performance improved in 75 cases with no improvement seen in 32, suggesting overall improvement of performance. ERNDIM diagnostic proficiency testing is a valuable activity which can help to assess laboratory performance, identify methodological/technical challenges, be informative during quality audits and contribute to a better clinical appreciation of diagnostic uncertainty.
Design of imprinting matrix for dual template sensing via electropolymerized polythiophene films
Authors: Singh Ritu , Singh Meenakshi 
Published: May 2022

AbstractThis work presents the design of 3‐thiophene acetic acid (3‐TAA) polymer matrix based molecularly imprinted polymer (MIP)/reduced graphene oxide (RGO) composite for sensitive and selective detection of antipyrine (AnP) and ethionamide (ETH) simultaneously. Dual drug embedded molecularly imprinted polymer (MIP) based electrochemical sensor was developed via electropolymerization of 3‐TAA. AnP and ETH were embedded inside a polymer matrix based on their 3‐D orientation and interaction(s) with functional monomer(s). Their extraction from polymeric matrix generates cavities complimentary to shape and size of AnP and ETH. The extraction of templates was confirmed by differential pulse voltammetry (DPV) as well as high‐performance liquid chromatography (HPLC). The designed sensor selectively captures and produces the electrochemical signal for imprinted drugs. The electrochemical behaviour of AnP and ETH was investigated by DPV technique. The sensitivity for both drug molecules was commendable on a single polymeric composite with RGO on GC electrode (LOD of 0.117 μM for AnP and 0.15 μM for ETH). Also, the sensor exhibited excellent selectivity towards AnP and ETH in the presence of other analogous interferent molecules. Thus, the designed sensor showed high sensitivity as well as high selectivity for imprinted dual drug molecules on a single platform.
Impact of Sustained Synovitis on Knee Joint Structural Degeneration: 4‐Year MRI Data from the Osteoarthritis Initiative
Authors: Ramezanpour Sara , Kanthawang Thanat , Lynch John , McCulloch Charles E. , Nevitt Michael C. , Link Thomas M. , Joseph Gabby B. 
Published: May 2022

BackgroundSynovial inflammation is a risk factor for osteoarthritis (OA). But to date, there is limited information on how inflammation impacts progression of knee OA.PurposeTo investigate how sustained synovitis, assessed with semi‐quantitative magnetic resonance imaging (MRI) scores, impacts progression of knee degenerative changes over 4 years.Study TypeRetrospective cohort study.SubjectsIn 249 participants (N = 132 women [53%]), from the Osteoarthritis Initiative (OAI) two definitions for synovitis were used resulting in two groups of participants with sustained synovitis at baseline, 2‐year, and 4‐year follow‐up (N = 80 and N = 132), and two groups without synovitis at all three time points (N = 81 and N = 47).Field Strength/Sequence3 T intermediate‐weighted (IW) turbo spin‐echo (TSE) sequence and three‐dimensional (3D) dual‐echo steady‐state (DESS) sequence.AssessmentSynovitis was scored semi‐quantitatively using the Anterior Cruciate Ligament Osteoarthritis Score (ACLOAS), MRI Osteoarthritis Knee Score (MOAKS), and synovial proliferation score (SPS). Two MRI‐based definitions of synovitis were used: (i) score ≥2 based on cumulative score of MOAKS and ACLOAS, and (ii) score ≥3 based on the cumulative score of ACLOAS, MOAKS, and SPS. Changes in structural abnormalities from baseline to year 4 measured using the whole‐organ MRI score (WORMS) were defined as outcomes.Statistical TestsLinear regression models were used to compare the differences in longitudinal changes in WORMS scores between participants with and without sustained synovitis for each definition of sustained synovitis. A P‐value of <0.05 was considered statistically significant.ResultsSignificantly higher rates of progression were found in participants with synovitis for patellar (Beta coeff. = 0.29) and medial tibial cartilage abnormalities (Beta coeff. = 0.29) for definition (i). For definition (ii), patellar (Beta coeff. = 0.36) and medial femoral cartilage (Beta coeff. = 0.30) abnormalities demonstrated significant differences.Data ConclusionGreater progression of structural degenerative disease was observed in individuals with sustained synovitis compared to those without sustained synovitis, suggesting that sustained synovitis is associated with progressive OA.Level of Evidence3Technical EfficacyStage 2
Directionally Sensitive Fractal Radiomics Compatible With Irregularly Shaped Magnetic Resonance Tumor Regions of Interest: Association With Osteosarcoma Chemoresistance
Authors: Djuričić Goran J. , Ahammer Helmut , Rajković Stanislav , Kovač Jelena Djokić , Milošević Zorica , Sopta Jelena P. , Radulovic Marko 
Published: May 2022

BackgroundComputational analysis of routinely acquired MRI has potential to improve the tumor chemoresistance prediction and to provide decision support in precision medicine, which may extend patient survival. Most radiomic analytical methods are compatible only with rectangular regions of interest (ROIs) and irregular tumor shape is therefore an important limitation. Furthermore, the currently used analytical methods are not directionally sensitive.PurposeTo implement a tumor analysis that is directionally sensitive and compatible with irregularly shaped ROIs.Study TypeRetrospective.SubjectsA total of 54 patients with histopathologic diagnosis of primary osteosarcoma on tubular long bones and with prechemotherapy MRI.Field Strength/SequenceA 1.5 T, T2‐weighted‐short‐tau‐inversion‐recovery‐fast‐spin‐echo.AssessmentA model to explore associations with osteosarcoma chemo‐responsiveness included MRI data obtained before OsteoSa MAP neoadjuvant cytotoxic chemotherapy. Osteosarcoma morphology was analyzed in the MRI data by calculation of the nondirectional two‐dimensional (2D) and directional and nondirectional one‐dimensional (1D) Higuchi dimensions (Dh). MAP chemotherapy response was assessed by histopathological necrosis.Statistical TestsThe area under the receiver operating characteristic (ROC) curve (AUC) evaluated the association of the calculated features with the actual chemoresponsiveness, using tumor histopathological necrosis (95%) as the endpoint. Least absolute shrinkage and selection operator (LASSO) machine learning and multivariable regression were used for feature selection. Significance was set at <0.05.ResultsThe nondirectional 1D Dh reached an AUC of 0.88 in association with the 95% tumor necrosis, while the directional 1D analysis along 180 radial lines significantly improved this association according to the Hanley/McNeil test, reaching an AUC of 0.95. The model defined by variable selection using LASSO reached an AUC of 0.98. The directional analysis showed an optimal predictive range between 90° and 97° and revealed structural osteosarcoma anisotropy manifested by its directionally dependent textural properties.Data ConclusionDirectionally sensitive radiomics had superior predictive performance in comparison to the standard nondirectional image analysis algorithms with AUCs reaching 0.95 and full compatibility with irregularly shaped ROIs.Evidence Level3Technical EfficacyStage 1
Discovery and pharmacodynamic evaluation of the novel butene lactone derivativeM355against influenza A virus in vitro and in vivo
Authors: Geng Jingwei , Hu Xiaoning , Zhang Zhongmou , Gu Zichen , Li Yuanyuan , Mou Xiaodong , Mao Lu , Ge Yongzhuang , Yang Xinyu , Song Yihui , Liu Hongmin , Wang Linqing , Wei Zhanyong , Wang Zhenya , Xu Haiwei 
Published: May 2022

AbstractA new series of butene lactone derivatives were designed according to an influenza neuraminidase target and their antiviral activities against H1N1 infection of Madin–Darby canine kidney cells were evaluated. Among them, a compound that was given the nameM355was identified as the most potent against H1N1 (EC50 = 14.7 μM) with low toxicity (CC50 = 538.13 μM). It also visibly reduced the virus‐induced cytopathic effect. Time‐of‐addition analysis indicated that H1N1 was mostly suppressed byM355at the late stage of its infectious cycle.M355inhibited neuraminidase in a dose‐dependent fashion to a similar extent as oseltamivir, which was also indicated by a computer modeling experiment. In a mouse model, lung lesions and virus load were reduced and the expression of nucleoprotein was moderated byM355. The enzyme‐linked immunosorbent assay and quantitative real‐time polymerase chain reaction analyses revealed that the levels of interferon‐γ, interferon regulatory factor‐3, Toll‐like receptor‐3, tumor necrosis factor‐α, interleukin (IL)‐1β, IL‐6, and IL‐8 were downregulated in theM355‐treated groups, whereas the levels of IL‐10 and IL‐13 were upregulated. Similarly, IgG was found to be increased in infected mice plasma. These results demonstrate thatM355inhibit the expression of H1N1 in both cellular and animal models. Thus,M355has the potential to be effective in the treatment of influenza A virus infection.
Relationship between flexibility and interfacial functional properties of soy protein isolate: succinylation modification
Authors: Lian Ziteng , Yang Sai , Dai Shicheng , Tong Xiaohong , Liao Peilong , Cheng Lin , Qi Weijie , Wang Yijun , Wang Huan , Jiang Lianzhou 
Published: May 2022

AbstractBACKGROUNDIn this paper, the effects of different succinic anhydride (SA) additions on the flexibility of soy protein isolate (SPI) were investigated, and changes in protein conformation and interfacial functional properties were measured. The structure–effect relationship between conformation, flexibility, and interfacial functional properties was established.RESULTSSPI was bound to SA through disulfide bonds, and the zeta potential was reduced. The β‐sheet content decreased, the disordered structure increased, and there were changes in tertiary structure and microstructure. The surface hydrophobicity, disulfide bond content, and solution turbidity were reduced to 5063, 1.0967 μmol g−1, and 0.0036 μmol g−1 respectively. The best flexibility of SPI (0.3977) and interfacial functional properties were obtained when the mass ratio of SA/SPI was 15%. Correlation analysis showed a highly significant positive correlation (P < 0.01) between flexibility and emulsification and foaming properties, with correlation coefficients of 0.960 and 0.942 for flexibility with emulsifying activity and emulsion stability respectively, and 0.972 and 0.929 for flexibility with foaming capacity and foaming stability respectively.CONCLUSIONThe results suggest that succinylation‐induced conformational changes of SPI improved its interfacial functional properties by changing its flexibility. These results provide theoretical guidelines for the development and application of highly emulsifiable and stable soy protein products utilizing succinylation. © 2022 Society of Chemical Industry.
Application of high‐pressure assisted thermal processing (PATP) at pilot scale for replacing conventional maturation and thermal cooking in whiteleg shrimp (Litopenaeus vannamei)
Authors: Puértolas Eduardo , Álvarez‐Sabatel Saioa , Montes Paula 
Published: May 2022

AbstractBACKGROUNDThe aim of this work was to examine for the first time the use of high‐pressure assisted thermal processing (PATP) (100, 350, 600 MPa; 100 °C; 3 min) at pilot scale for replacing shrimp (Litopenaeus vannamei) maturation and cooking, analyzing its impact on peeling yield, color, texture and sensory properties. Shrimps subjected to conventional maturation (ice; 2 days) and thermal cooking (100 °C; boiling water, 3 min) were used as controls.RESULTSPATP treatments at 100–350 MPa improved manual peelability over the control (P ≤ 0.05), maintaining similar peeling yield, color (L*, a*, b*), texture (shear force, shear work) and sensory properties (appearance before and after peeling, flavor, firmness; P < 0.05). However, increasing pressure to 600 MPa clearly overprocessed the samples, making it impossible to remove all the meat from the shell and resulting in a softer texture, 4.1% lower peeling yield and worse sensory quality (P ≤ 0.05).CONCLUSIONPATP (< 350 MPa; 100 °C) could be an alternative to replace conventional maturation and thermal cooking in the production of cooked shrimps, reducing processing time from days to minutes by performing both processes in a single step. © 2022 Society of Chemical Industry.
Percutaneous lumbar annular puncture: A rat model to study intervertebral disc degeneration and pain‐related behavior
Authors: Wawrose Richard A. , Couch Brandon K. , Dombrowski Malcom , Chen Stephen R. , Oyekan Anthony , Dong Qing , Wang Dong , Zhou Chaoming , Chen Joseph , Modali Karthik , Johnson Marit , Sedor‐Schiffhauer Zachary , Hitchens T. Kevin , Jin Tao , Bell Kevin M. , Lee Joon Y. , Sowa Gwendolyn A. , Vo Nam V. 
Published: May 2022

AbstractBackgroundPrevious animal models of intervertebral disc degeneration (IDD) rely on open surgical approaches, which confound the degenerative response and pain behaviors due to injury to surrounding tissues during the surgical approach. To overcome these challenges, we developed a minimally invasive percutaneous puncture procedure to induce IDD in a rat model.MethodsTen Fischer 344 male rats underwent percutaneous annular puncture of lumbar intervertebral discs (IVDs) at L2‐3, L3‐4, and L4‐5. Ten unpunctured rats were used as controls. Magnetic resonance imagings (MRIs), serum biomarkers, and behavioral tests were performed at baseline and 6, 12, and 18 weeks post puncture. Rats were sacrificed at 18 weeks and disc histology, immunohistochemistry, and glycosaminoglycan (GAG) assays were performed.ResultsPunctured IVDs exhibited significant reductions in MRI signal intensity and disc volume. Disc histology, immunohistochemistry, and GAG assay results were consistent with features of IDD. IVD‐punctured rats demonstrated significant changes in pain‐related behaviors, including total distance moved, twitching frequency, and rearing duration.ConclusionsThis is the first reported study of the successful establishment of a reproducible rodent model of a percutaneous lumbar annular puncture resulting in discogenic pain. This model will be useful to test therapeutics and elucidate the basic mechanisms of IDD and discogenic pain.
A novel framework for evaluating the effect of vegetation restoration via grazing exclusion by fencing: A case‐study from the Qinghai–Tibet Plateau
Authors: Yang Wanjing , Zhang Daojun , Luo Gang 
Published: May 2022

AbstractGrazing exclusion by fencing (GEF) has been implemented to prevent the deterioration of grassland ecosystems in China since the beginning of this century; meanwhile, the effects have attracted widespread attention from the academic community. However, due to the simultaneity of different factors, it is difficult to separate the effect of different policies from natural resource endowments and identify the independent role of each policy. In this study, a novel framework was established based on spatiotemporal statistics. First, an indicator‐denoted vegetation restoration potential achievement degree (VRPAD) was introduced to lessen the impact of resource endowment conditions. Second, a double‐difference model of both space and time was developed to reflect the net improvement of VRPAD brought by GEF. The case study showed that 15 out of the 17 fenced‐off enclosures achieved positive VRPAD growth since the GEF implementation, while only 58.8% showed a much faster improvement or a slower degeneration than their adjacent areas. It was also found GEF effect presented apparent administrative differences in terms of spatial distribution. The number of effective fences accounted for 75% in Mami Town, but it was 0 in Chabu Town. It is concluded that the role of GEF may be overestimated, multi‐pronged approaches are more conducive to vegetation restoration, and strengthening grassroots management and self‐policing of fences is important to improve GEF efficiency. This research provides a novel framework for distinguishing policy effect and is expected to inspire new ideas and methodological support for policy formulation and evaluation.
Assessing soil system changes under climate‐smart agriculture via farmers' observations and conventional soil testing
Authors: Eze Samuel , Dougill Andrew J. , Banwart Steven A. , Sallu Susannah M. , Mgohele Rashid N. , Senkoro Catherine J. 
Published: May 2022

AbstractSoil degradation remains a challenge in African highlands, where land management lacks a strong context‐specific evidence base. We investigated the impacts of recently implemented soil and water conservation (SWC) practices—farmyard manure addition, incorporation of crop residues in soil andfanya juuterracing under an agroforestry system on soil health indicators in the East Usambara Mountains of Tanzania. Farmers' observations of soil changes were combined with conventional soil testing to assess the initial impacts of SWC practices relative to conventional non‐SWC practice. Majority of farmers (66%–83%) reported that combiningfanya juuterracing with organic amendments led to soil colour change from red to black and an increase in crop yield. Despite the observed darkening of the soil, there was no significant increase in soil organic carbon stock and the contents of N, P, K. There were important changes in soil physical properties, including greater aggregate stability (mean weight diameter of 1.51–1.71 mm) in the SWC plots, a greater volume of transmission pores (>60 μm) and coarse storage pores (10–60 μm) in the surface soil layer (0–15 cm), and greater volume of fine storage pores (0.2–10 μm) and residual pores (0.2 μm) in the sub‐surface layer (15–30 cm) of the SWC plots compared with the conventional plots. These changes indicate that SWC rapidly enhances infiltration and retention of water within the root zone, which are important for increasing crop yields and improving the resilience of the agro‐ecosystem to environmental stress. Combining SWC with effective soil fertility management is needed for sustainable highland agriculture.
Stakeholder engagement infrastructure to support multicenter research networks: Advances from the clinical research networks participating in PCORnet
Authors: Cope Elizabeth L. , McTigue Kathleen M. , Forrest Christopher B. , Carton Thomas W. , Fair Alecia M. , Goytia Crispin , Harrington Joseph M. , Lowe Susan , Merritt J. Greg , Shenkman Elizabeth A. , Stephens William J. , Templeton Anna , Williams Neely A. , Zemon Nadine , Millender Sarah , Angove Rebekah S. M. 
Published: May 2022

AbstractBackgroundThe evidence based on the inclusion of patients and other stakeholders as partners in the clinical research process has grown substantially. However, little has been reported on how stakeholders are engaged in the governance of large‐scale clinical research networks and the infrastructure used by research networks to support engagement in network‐affiliated activities.ObjectivesThe objective was to document engagement activities and practices emerging from Clinical Research Networks (CRNs) participating in PCORnet, the National Patient‐Centered Clinical Research Network, specifically regarding governance and engagement infrastructure.MethodsWe conducted an environmental scan of PCORnet CRN engagement structures, assets, and services, focusing on network oversight structures for policy development and strategic decision‐making. The scan included assets and services for supporting patient/stakeholder engagement. Data were collected by searching web‐based literature and tool repositories, review of CRN Engagement Plans, analysis of previously collected key informant interviews, and CRN‐based iterative review of structured worksheets.ResultsWe identified 87 discrete engagement structures, assets, and services across nine CRNs. All CRNs engage patients/stakeholders in their governance, maintain workgroups and/or staff dedicated to overseeing engagement strategies, and offer one or more services to non‐CRN researchers to enhance conducting engaged clinical research.ConclusionsThis work provides an important resource for the research community to explore engagement across peers, reflect on progress, consider opportunities to leverage existing infrastructure, and identify new collaborators. It also serves to highlight PCORnet as a resource for non‐CRN researchers seeking to efficiently conduct engaged clinical research and a venue for advancing the science of engagement.