bluuebunny/crossref_metadata_2025_split huggingface dataset:
EFFECT OF STIMULATING ORGANISM WITH BACTERIAL LIPOPOLYSACCHARIDE ON DEVELOPMENT OF OXIDATIVE STRESS IN BICEPS FEMORIS OF RAT UNDER MODELLED METABOLIC SYNDROME
Authors: Akimov O.Y. , Mykytenko A.O. , Mischenko A.V. , Kostenko V.O. 
Published: November 2022

Metabolic syndrome is one of the most widespread non-infectious pathologies in the world. Metabolic syndrome is quite often accompanied by an increase in body weight and can lead to the development of type 2 diabetes. Between 1990 and 2015, global mortality associated with high body mass index increased by 28.3%.
The purpose of this work is to determine the activity of antioxidant enzymes, the production of superoxide anion radical, the content of oxidatively modified proteins and the concentration of malondialdehyde in the biceps femoris of rats under experimental metabolic syndrome and stimulation of the organism with bacterial lipopolysaccharide.
The study was conducted on 24 sexually mature male Wistar rats weighing 200-260 g. The animals were divided into 4 groups of 6 animals each. The first group was the control group. The second group was the test metabolic syndrome group. Metabolic syndrome was modelled by using a 20% fructose solution as the only source of water for 60 days. The third group received S. typhi bacterial lipopolysaccharide in the first week in a dose of 0.4 μg/kg intraperitonealy three times a week, then once a week throughout the experiment. The fourth group underwent the combined exposure to lipopolysaccharide and metabolic syndrome. The development of oxidative stress was observed in the three experimental groups. During modelling of the metabolic syndrome, oxidative stress was characterized by an increase in the production of the superoxide anion radical whilst the activity of the studied antioxidant enzymes decreased. During bacterial lipopolysaccharide stimulation, oxidative stress was characterized by an increase in the production of superoxide anion radical under an increase in superoxide dismutase activity and a decrease in catalase activity. Combined stimulation of the organism by bacterial lipopolysaccharide and modelled metabolic syndrome on the oxidative stress was characterized by an increase in the production of the superoxide anion radical under an increase in the activity of the studied antioxidant enzymes.
The combined stimulation by bacterial lipopolysaccharide and modelled metabolic syndrome leads to the development of oxidative damage to the lipid and protein components of biceps femoris that is a consequence of an increase in the production of reactive oxygen species under a compensatory increase in the activity of antioxidant enzymes.
CHANGES IN ADHESIVE PROPERTIES OF CLINICAL ISOLATES OF STREPTOCOCCUS MITIS AFTER EXPOSURE TO ANTISEPTICS
Authors: Chumak Yu.V. , Loban G.A. , Ananieva M.M. , Faustova M.O. , Havryliev V.M. 
Published: November 2022

The resident oral microbiota plays an important role as a factor in its colonization resistance that ensures human oral health. However, at the same time, oral microorganisms can act as causative agents of infectious diseases. Scientists emphasize that in the situation when bacteria demonstrated increased resistance to antibiotics, quorum sensing is a particularly attractive target, since this mechanism controls several factors of bacterial virulence that affect adhesion, invasion and colonization of microorganisms during the development of the pathological process. Quorum sensing inhibitors can be enzymes and various chemicals that able to affect quorum sensing. Streptococcus mitis is a representative of the resident microbiota of the human oropharynx. But despite its commensalism, Streptococcus mitis in association with other microorganisms takes part in many pathological processes. Objective of this study is to study the effect of Dekasan and Iodoform antiseptics on the adhesive properties of Streptococus mitis clinical isolates taken from the oral cavity of patients with infectious and inflammatory post-extraction complications.
Materials and methods: examined clinical isolates of Streptococus mitis, which were isolated from 8 patients undergoing treatment for infectious-inflammatory post-extraction complications in the medical and surgical department of Poltava Regional Centre of Stomatology - Dental Clinical Polyclinic. Identification of selected cultures was carried out using an automatic bacteriological analyzer Vitec-2 compact bioMarieux (France). We used the average values of subbacteriostatic concentrations of the active ingredients of antiseptics. Adhesive properties of clinical isolates under the influence of Dekasan and Iodoform were determined according to the method of V.I. Brylis.
Conclusions: antiseptics have different effects on the adhesive properties of clinical isolates of Streptococus mitis.
MODERN METHODS OF ROSACEA TREATMENT
Authors: Bezeha O.V. , Yemchenko Y.O. , Vasylyeva K.V. , Oliinichenko Ya.O. , Kravchenko V.H. 
Published: November 2022

Rosacea is a chronic inflammatory dermatosis that mainly affects the cheeks, nose, chin and forehead. Rosacea usually begins between the ages of 30 and 50, but can occur at any age. Population prevalence rates range from < 1 to 22%, but these percentages are likely influenced by differences in study design, methodology, population, geographic location, and cultural and social differences. Rosacea is characterized by recurrent episodes of flushing or transient erythema, persistent erythema, phymatous changes, papules, pustules, and telangiectasia, and the eyes may be involved. Due to the fact that rosacea affects the face, patients with this pathology often have low self-esteem and are prone to anxiety and depression, because this disease has a cosmetic defect. In addition to general skin care, there are several approved treatment options available to address these issues, both topical and systemic. For some functions, intense pulsed light, laser, and surgery are valuable. Recent advances in basic science have highlighted the role of the innate and adaptive immune system, as well as neurovascular dysregulation, underlying the spectrum of clinical manifestations of rosacea. Endogenous and exogenous stimuli can initiate and exacerbate multiple pathways in patients with rosacea.
With the updated diagnosis and classification of rosacea, treatment options for patients with rosacea have attracted the attention of dermatologists. Here, we summarize the latest advances in the treatment of rosacea, including skin care and cosmetic procedures, topical therapy, oral therapy, laser and light therapy, injection therapy, and combination therapy.
FEATURES OF PATHOGENESIS, COURSE AND THERAPY OF PNEUMONIA UNDER COVID-19 (Literature Review)
Authors: Zhdan V.M. , Babanina M.Y. , Boriak Kh.R. , Kiriyan E.A. , Tkachenko M.V. 
Published: November 2022

Having analyzed the latest recommendations of the World Health Organization, the new data from the scientific literature, and current treatment guidelines, the latest epidemiological data of identified different cases of this infection according to epidemiological and clinical criteria were presented. The main pathogenetic signs, risk factors, including inter-individual differences, which can be represented by the host's genetic profile, are highlighted. The main clinical signs and their distribution, classification according to the severity of the disease, features of the course and treatment of pneumonia in human coronavirus infections are shown, including indications for antibacterial therapy and the main criteria of oxygen support.
The basic WHO laboratory recommendations for patients with acute respiratory infection who have been in contact with patients having a confirmed or probable case are defined. The main hardware methods of diagnosis, modern approaches in the therapy in the absence of specific antiviral agents are presented. The lack of definitive specific treatment for this disease and the future development of such a pandemic remains unpredictable that indicates the need for strict adherence to classic public health recommendations, the importance of vaccination. The outbreak of COVID-19 in this century once again had emphasized the constant threat of infectious diseases spread by pathogenic viruses among humanity and this requires effective global cooperation and a high level of preparedness.
IMPACT OF SLEEP QUALITY ON QUALITY OF LIFE IN PATIENTS WITH ANXIETY DISORDERS
Authors: Zhyvotovska A. I. 
Published: November 2022

Modern scientific sources point out a close connection between depression, anxiety, psychological distress and low quality of life. The purpose of this study is to assess the impact of sleep quality on the quality of life in patients with anxiety disorders of neurotic origin. Examination of 81 patients with anxiety disorders of neurotic origin and dyssomnic disorders was carried out using clinical-psychopathological, psychodiagnostic and statistical research methods. The psychodiagnostic method included determining the level of stress load according to the PSM-25 questionnaire, the level of anxiety and depression by the HADS scale, the severity of the mental and somatic component of anxiety according to the HAM-A scale, assessing the quality of life according to the "SF-36" scale, studying sleep quality disorders according to the PSQI questionnaire.
Depending on the level of psychological stress based on the PSM-25 scale, 3 groups were formed: group 1 included 25 (30.7%) people with a low level of stress, group 2 included 29 (35.8%) individuals with an average stress level, and group 3 included 27 people (37.4%) with a high stress level. Analysis according to the HADS scale has shown that anxiety symptoms prevail over depressive symptoms in all examined subjects. According to the HAM-A scale, the average score of the somatic and mental components in the group 1 is significantly lower compared to the group 2 (p<0.001) and the group 3 (p<0.001).
This study has demonstrated that as the level of stress increases, there is a tendency for quality of life indicators to decrease in all spheres of functioning. Significantly worse sleep quality scores were found in patients with anxiety disorder and high mental stress level compared to those with moderate or low levels. There is a strong negative correlation between indicators by the "vital activity" scale and such components of sleep quality as "daytime dysfunction", "sleep latency" and the total score of sleep quality, between indicators by the scales "role functioning due to physical condition" and "daytime dysfunction". It has been found out that with increasing pain intensity, all indicators of sleep quality decline. The data obtained should be taken into account in developing patient-centred treatment plan for this group of patients.
ROLE OF HOMOCYSTEINE IN DEVELOPMENT OF ABDOMINAL PAIN IN PATIENTS WITH IRRITABLE BOWEL SYNDROME AND CONSTIPATION
Authors: Kryvoruchko I.G. , Skrypnyk І.М. , Maslova G.S. , Lymanets T.V. , Кudria I.P. 
Published: November 2022

Introduction. Irritable bowel syndrome with constipation poses a serious medical and social problem. Studying the pathogenetic mechanisms of irritable bowel syndrome with constipation may improve therapeutic approaches and patient’s quality of life.
The aim of this study is to investigate the homocysteine role in the development of abdominal pain syndrome in patients with irritable bowel syndrome and constipation, taking into account body mass index.
Subjects and methods. The study involved 55 patients with functional bowel disorders, who were divided into 2 groups: group I (n=27) included patients with functional constipation; II group (n=28) involved patients with irritable bowel syndrome and constipation. The average age of patients in group I was 35.7±8.63 years, in group II – 34.50±9.06 years, the ratio of men and women was 7(25.9%) / 20(74.1%) and 10 (35.7%) / 18(64.28%), respectively. A modified faces pain scale was used to assess pain intensity. Height, weight, and waist circumference were measured, body mass index was calculated according to formula: body mass index = weight (kg)/height (m2). The homocysteine level in the blood serum was measured.
Results. Abdominal obesity with the waist circumference greater than 88 cm for women and greater than 102 cm for men was recorded in 6 (21.42%) patients with irritable bowel syndrome and constipation and in 4 (14.81%) patients with functional constipation. The severity of pain and meteorism according to the modified faces scale in patients with irritable bowel syndrome with constipation were respectively 2.4 and 1.7-fold (р˂0.0001) higher than the respective parameters in the patients with functional constipation.
The content of homocysteine in the blood serum in the patients with irritable bowel syndrome and constipation was 1.9-fold (р˂0.0001) higher than in the patients with functional constipation. Increased homocysteine level led to the increase in the risk of developing irritable bowel syndrome and constipation (RR=3.61; 95%CI 1.37–9.52; p<0.01). It is important to stress that the serum homocysteine level in the patients with irritable bowel syndrome with constipation was directly correlated with the pain severity assessed by the modified faces pain scale (r=+0.9; p=0.0001).
Conclusion. The severity of abdominal pain and meteorism in patients with irritable bowel syndrome and constipation is associated with the homocysteine level in the blood serum.
CONCOMITANT SOMATIC DISEASES IN CHILDREN WITH AUTISTIC SPECTRUM DISORDERS: CHALLENGING ISSUES OF MEDICAL OBSERVATION BY PEDIATRICIANS (LITERATURE REVIEW)
Authors: Melashchenko O.I. , Kaliuzhka O.O. , Fastovets M.M. , Ziuzina L.S. , Kozakiewich V.K. 
Published: November 2022

An analysis of modern medical literature carried out to study the main concomitant pathology in children with autism spectrum disorders gas revealed what concomitant diseases are more common in children with autism than in the general population. The structure of concomitant somatic pathology of children with autism includes disturbances from the gastrointestinal tract, sleep disturbances, the risk of overweight and obesity that affects the quality of life and complicates the manifestations of the main disease. The review of the current scientific literature has provided preliminary evidence that targeting the gut ecosystem has the potential to influence both autism spectrum disorders symptoms and gastrointestinal symptoms. The use of prebiotics and probiotics to correct behaviour and treat gastrointestinal diseases in children with autism has had limited effectiveness. The effectiveness and safety of using a gluten-free and casein-free diet in autism spectrum disorder is remaining controversial: a number of studies have reported no significant changes in autism symptoms, and the risk of developing nutrient deficiencies limits its clinical use. A combination of behavioural interventions and the use of the paediatric slow-release melatonin molecule are effective in the treatment of sleep disorders in children with autism.
Conclusion. The high prevalence of concomitant somatic pathology in children with autism affects the course of the main disease, requiring a comprehensive examination of these children for its timely diagnosis and treatment. Taking into account the polyetiological nature of the etiopathogenetic mechanism of the development of autism spectrum disorder, the treatment of children with autism by a paediatrician should be carried out using evidence-based methods, with multidisciplinary support by specialists in the profile of existing concomitant disorders.
Secure Image Steganography by Utilizing DNA Properties
Authors:   Department of Computer sciences College of Computer Sciences and Math University of Mosul, Ismael Yaseen 
Published: November 2022

In the last period, Steganography is commonly used as an alternative to encryption to achieve secret communication between parties. Many methods have emerged to achieve steganography, including the use of spatial domain, spread spectrum, transform domain, and etc.	On the other hand, the methods of attackers have also developed in revealing hidden data and trying to retrieve it. To increase the security of the hiding process, some researchers have found hybrid methods that combine encryption and steganography processes. The research aims to present a new method in steganography by taking advantage of the properties of DNA, which includes the random sequence of nitrogenous bases (A, C, G, T), the process of hybridization, which occurs between two single strands of DNA to form a double strand of DNA so that the bases in the first strand are complementary to the nitrogenous bases in the second strand. The research includes the following steps: First, the secret image to be hidden is encrypted by encoding it into a series of nitrogenous bases, and then the XOR process is performed with a nitrogenous bases sequence for a DNA tape agreed upon between the sender and recipient, the hybridization process applied before and after the XOR process. The results show that encrypted image is much different from the original image and thus they added another level of security to the hidden image. Secondly, the encrypted image resulting from the first step is hidden in the cover image and using a new method based on the use of the agreed-upon DNA tape as a key.
Decontamination of Two Umbilical Cord Blood Grafts Prior to Autologous Administration
Authors: Stancioiu Felician , Bogdan Raluca , Bogdan Bulumac , Ivanescu Bogdan , Dumitrescu Radu 
Published: November 2022

Umbilical cord blood (UCB) is proving to be a valuable resource of stem cells and currently is being used for a variety of oncological and hematological pathologies and metabolic disorders; in addition, new clinical trials are showing promising results in certain neurological, autoimmune and developmental disorders. More recently the Food and Drug Administration – FDA - has granted approval for the clinical use of cellular therapies with UCB-based products and new therapeutic utilizations are being studied for regenerative medicine; all these developments will increase the utilization of “off-the-shelf” UCB units.

As a drawback, contamination of UCB grafts is a significant occurrence (upwards of 5% in most analyses), and even though it consists mainly of non-pathogenic bacteria it can raise serious questions regarding intravenous UCB administration, especially in patients who are not receiving coincidental antibiotic coverage.

Here we report the successful decontamination of two UCB grafts prior to administration without compromising the viability of the stem cells administered, and propose to apply the same principle and procedure to any contaminated graft.
Selection of an engineering institution: Students’ perceptions of choice characteristics and suitability under the COVID-19 pandemic
Authors: Mahajan Prashant , Patil Vaishali 
Published: November 2022

BACKGROUND

COVID-19 has impacted Indian engineering institutions (EIs) enormously. It has tightened its knot around EIs that forced their previous half-shut shades completely down to prevent the risk of spreading COVID-19. In such a situation, fetching new enrollments on EI campuses is a difficult and challenging task, as students’ behavior and family preferences have changed drastically due to mental stress and emotions attached to them. Historically, during crisis situations, institutions have struggled to return to the normal track. Consequently, it becomes a prerequisite to examining the choice characteristics influencing the selection of EI during the COVID-19 pandemic to make it ‘normal’ for ‘new’ enrollments.

The purpose of this study is to critically examine choice characteristics that affect students’ choice for EI and consequently to explore relationships between institutions’ characteristics and the suitability of EI during the COVID-19 pandemic across students’ characteristics. Quantitative research, conducted through a self-reported survey composed of a closed-ended structured questionnaire, was purposefully incorporated into the students who recently were enrolled in EIs (batch years 2020-2021) belonging to the North Maharashtra region of India.

RESULTS

The findings of this study revealed dissimilarities across students’ characteristics regarding the suitability of EIs under pandemic conditions. Regression analysis revealed that EI characteristics such as proximity, image and reputation, quality education and curriculum delivery have significantly contributed to suitability under COVID-19. At the micro level, multiple relationships were noted between EI characteristics and the suitability of EI under the pandemic across students’ characteristics.

CONCLUSION

Bringing ‘normality’ to ‘new’ enrollments totally depends on EI’s resilience in meeting the needs of diversity in the COVID-19 pandemic situation, which repositions themselves to govern student-centric strategies instituted for the overall suitability of EI under pandemic conditions. The study has successfully demonstrated how choice characteristics can be executed to regulate the ‘suitability’ of EI under the COVID-19 pandemic for the inclusion of diversity. It is useful for policymakers and academicians to reposition EIs that fetch diversity during the pandemic. This study is the first to provide insights into the performance of choice characteristics and their relationship with the suitability of EIs under a pandemic and can be a yardstick in administering new enrollments.
Perancangan Ilustrasi Sampul Buku Kompilasi Folktale Lintas Budaya “Rove the Lore: The Endless Journey to Unveil the Myths”
Authors: Sutanto Shienny Megawati , Suhartono Natasya Priscilla 
Published: November 2022

Abstrak Folktale adalah kejadian yang diceritakan secara turun temurun antar generasi atau karya-karya tradisional, yang dikenal, dan dimiliki setiap orang atau masyarakat. Folktale dipenuhi dengan kisah kearifan lokal, wisdom, cinta kasih, atau sebuah mimpi dari masyarakat dan komunitas, yang kadang menjadi pegangan hidup dari masyarakat setempat. Melihat situasi saat ini tidak dapat dipungkiri bahwa cerita-cerita tersebut mulai ditinggalkan oleh masyarakat. Oleh karena itu, penelitian ini bertujuan merancang buku yang mengangkat keragaman budaya folktale melalui projek lintas negara antara mahasiswa desain komunikasi visual dari Universitas Ciputra dengan Universitas Coventry. Metode yang digunakan untuk perancangan melalui dua tahap yaitu Tahap Persiapan dan Tahap Eksekusi Desain. Fokus perancangan pada ilustrasi sampul buku Rove the Lore dimana isi buku merupakan hasil visualisasi folktale yang dibuat oleh mahasiswa peserta. Hasilnya adalah ilustrasi sampul berupa siluet manusia yang melambangkan pembaca sedang menjelajahi hutan penuh dengan folktale yang hampir “punah”. Hasil akhir buku yang dibuat adalah buku berjenis coffee table book berukuran 21 x 27.5 cm, tebal buku 53 halaman dan dijilid dengan teknik soft cover. Kata kunci: folktale, ilustrasi, lintas budaya, perancangan, sampul buku AbstrakFolktales are events that are told from generation to generation or traditional works, which are known and owned by everyone or society. Folktale is filled with stories of local wisdom, wisdom, love, or a dream from the people and the community, which sometimes become the way of life of the local community. At this time, it is undeniable that these stories are starting to be abandoned by the community. Therefore, this study aims to design a book that elevates the diversity of folktale culture through a cross-country project between visual communication design students from Ciputra University and Coventry University. The design method used is the Preparation Stage and the Design Execution Stage. The design focus is on the cover illustration for the book Rove the Lore where the contents of the book are the result of a folktale visualization created by the participating students. The result is a cover illustration of a human silhouette that symbolizes the reader exploring a forest full of endangered folktales. The final result of the book is a coffee table book with a size of 21 x 27.5 cm, a book thickness of 53 pages and is bound using the soft cover technique. Keywords: book cover, cross culture, design, folktale, illustration