bluuebunny/crossref_metadata_2025_split huggingface dataset:
Culture-dependent evaluation of the respiratory microbiome in children with cystic fibrosis
Authors: Ishchenko Oksana , Koshova Iryna , Krushinska Tetiana , Kolesnikova Iryna , Stepanskyi Dmytro 
Published: November 2022

The study aimed to assess the regional peculiarities of the respiratory profile of children with cystic fibrosis (CF) in the Dnipro region (Ukraine).
Methods. Children living in the Dnipro region and aged younger than 18 years old with molecular-genetic confirmation of CF were enrolled in the study. Lung colonization was evaluated using a culture-dependent method. Sputum, mucus from the posterior pharyngeal wall and bronchoalveolar lavage fluid (BALF) were utilized.
Results. The Firmicutes phylum was the most common and occupied 54.00 % of the general proportion. On the other hand, the Proteobacteria phylum demonstrated overexpression in CF airways and kept the second rank with 28.87 %.
Sorensen's species similarity coefficient showed an allied affinity between the microbial burden of oropharyngeal samples with nasopharyngeal and sputum, QS = 0.61 and 0.91, respectively. However, the species composition within the nasal cavity was distinct from sputum and BALF (QS=0.47).
The primary pathogens in childhood were S. aureus, H. influenza, P. aeruginosa and A. fumigatus. In contrast to gram-negative non-fermenters (GNNF), the prevalence of S. aureus isolates by age had a non-linear character. The commensal microbiota changed negatively with age. Among children under 12 years, the Streptococcus genus was identified in 23.08 % of the samples, but among the age category older than 15 – only in 9.22 %.
11.06 % of S. aureus had small colony variants (SCVs) morphotypes. Isolates of P. aeruginosa with the properties of SCVs were also found in children who underwent prolonged antimicrobial treatment. However, the most prominent was the mucoid phenotype – 34.31 % of isolates.
Conclusions. Along with conventional microbiological properties, obligate pathobionts in children with CF exhibited changes, resulting in difficulties in identification. These included auxotrophic modification into SCVs and mucoid transformation.
The culture-dependent technique gives crucial data about the profile of pathogens usually associated with CF, although it is sufficiently limited
Aspects of sexual life that are associated with the occurrence of depressive disorders and autonomic dysfunction among medical students of National Pirogov Memorial Medical University, Vinnytsya
Authors: Kucherenko Oksana , Chaika Hryhorii 
Published: November 2022

Depressive disorders are widespread nowadays. First of all, this was affected by social isolation related to covid-19, and starting from 2022 – the war in our country. Depression and sexual life are connected and important, because a person with a depressive disorder cannot fully live a social and sexual life. As a result, the birth rate in the country and the population in general decreases. Therefore, the issue of studying the occurrence of depressive disorders in young sexually active and inactive people is extremely relevant.
The aim. The main objective of this work was to explore the different areas of sexual life of residents and its association with mental health. Additionally, differences in sexual life and mental health (depression) based on gender were also analyzed.
Materials and methods. An anonymous survey of 506 medical students of NPMMU, V has been conducted using a questionnaire that included 25 questions on various aspects of the sexual life of the respondents. The analysis of the obtained results of the study was carried out on the basis of the computer programme “Statistica 6.1” using nonparametric methods to evaluate obtained results.
The results. We found a statistically significant association between sexual activity and condition of mental health. Results regarding young adults, presented lower levels of sexual satisfaction and greater depression, whereas young women also exhibited higher levels of depression.
Conclusions. Sexual life has a positive effect on the mental and psychological health of respondents. This information should be conveyed to young people.
Eksplorasi Makna Kematian dan Kehidupan Melalui Tafsir Naratif Kisah Lazarus
Authors: Margaret Carmia 
Published: November 2022

AbstractWorks of narrative analysis on the Lazarus’s story in the Gospel of John tend to see the progressions of its plots and characters as flat. This paper proposes an improvisation in the narrative approach of Lazarus’s story, which traces plots and characters within the thematic framework of “death” and “life.” Each character in the story experiences its own “death,” namely physical death; death by disbelief in Jesus; or deadly deep sorrow; but then Jesus gave way to “life,” through the promise of the bodily and eternal resurrection, the promise of salvation by faith in His name, and the promise of consolation in deep sorrow.
AbstrakPenelitian-penelitian naratif yang dilakukan terhadap kisah Lazarus dalam Injil Yohanes cenderung memandang datar progresi plot dan tokoh di dalamnya. Makalah ini mengusulkan sebuah improvisasi dalam kritik naratif kisah Lazarus, yaitu menelusuri plot dan tokoh dalam bingkai kerangka tema “kematian” dan “kehidupan.” Setiap tokoh dalam cerita mengalami “kematian” masing-masing, yaitu kematian ragawi; kematian karena ketidak-percayaan kepada Yesus; atau kematian karena dukacita mendalam; tetapi kemudian Yesus memberikan jalan menuju “kehidupan,” melalui janji kebangkitan tubuh dan hidup yang kekal, janji keselamatan oleh iman dalam nama-Nya, dan janji belarasa dalam dukacita.
Arketipe Kepribadian Naomi: Suatu Kajian Psikoanalitikal Carl Gustav Jung
Authors: Kasemetan Feliana Eliza , Ranimpi Yulius Yusak , Rungkat Merry Kristina 
Published: November 2022

AbstractNaomi is one of the characters in the book of Ruth who experiences many challenges. The death of her husband and two sons left Naomi as a widow. Naomi has to survive with the status as a widow who haslimitations in social circles. This paper aims to analyze and describe Naomi’s personality archetypes using Jung’s psychoanalytic studies. The method used is descriptive analytic which is traced through historical traces, characterizations and conversations in the story. The results of the study prove that Naomi is able to survive and has the support of the archetypes contained within her. The archetypes are: anima-animus, shadow, persona, self, and the great mother. With the support of archetypes, Naomi is able to survive and show her bestqualities.
AbstrakNaomi adalah salah satu tokoh dalam kitab Rut yang mengalami banyak tantangan. Kematian suami dan kedua putra membuat Naomi hidup menjanda. Naomi harus bertahan hidup dengan status janda yang memiliki keterbatasan dalam lingkungan sosial. Tulisan ini bertujuan untuk menganalisis dan mendeskripsikan arketipe kepribadian Naomi menggunakan kajian psikoanalitikal Jung. Metode yang dipakai adalah deskriptif analitik yang ditelusuri melalui jejak historis, penokohan dan percakapan dalam cerita. Hasil penelitian membuktikan bahwa Naomi mampu bertahan hidup dan mendapat dukungan dari arketipe yang terdapat dalam dirinya. Arketipe tersebut adalah: animus (pikiran), shadow (bayangan), persona (topeng), self (diri), dan the great mother (ibu agung). Adanya dukungan dari arketipe membuat Naomi mampu bertahan hidup dan menunjukkan kualitas terbaik dirinya.
Teo Ekologi Kontekstual dalam Titik Temu antara Kejadian 1:26-31 dengan Konsep Sangkan Paraning Dumadi dalam Budaya Jawa
Authors: Panjaitan Firman 
Published: November 2022

AbstractThis article supports the criticism of anthropocentrism as a factor in the environmental crisis. Using the cross-textual reading method, especially looking for similarities between the two texts in a balanced comparison, this study demonstrates the integration of humans and nonhuman creatures in the community of God. References for this thought are found in Genesis 1:26-31 and the Javanese philosophy of Sangkan Paraning Dumadi, a concept about cosmic consciousness asserting that God, human being and nature are one entity. This study attempts to build a contextual theo-ecology by examining the two texts equally. This resulted in a theological awareness that degrades anthropocentrism and replaces it with cosmocentrism emphasizing the unity of humans and non-humans as mutually sustaining God’s creatures.
AbstrakArtikel ini mendukung kritik terhadap antroposentrisme sebagai faktor penyebab krisis lingkungan. Menggunakan metode pembacaan lintas tekstual, khususnya mencari kesamaan kedua teks dalam perbandingan yang seimbang, artikel ini memperlihatkan bahwa manusia dan alam tergabung dalam komunitas Allah. Acuan dari pemikiran ini terdapat dalam Kejadian 1:26-31 dan filosofi Sangkan Paraning Dumadi, sebuah konsep tentang kesadaran kosmik yang menegaskan bahwa Allah, manusia dan alam adalah satu kesatuan. Melalui penelitian dua teks secara berimbang, artikel ini berupaya untuk membangun sebuah teoekologi kontekstual. Hasilnya adalah kesadaran teologis yang mendegradasi antroposentrisme dan menggantikannya dengan kosmosentrisme, yang menekankan kesatuan manusia dan alam sebagai ciptaan Allah yang saling melestarikan.
Photocatalysis coupled with adsorption of AC@Ni0.5Cu0.5Fe2O4 in peroxydisulfate assisted system efficiently enhance ciprofloxacin removal
Authors: Wang Bo , Cao Xuan , Li Shifeng , Yao Shuhua , Wang Haibo 
Published: November 2022

Abstract
Nickle-copper ferrite (Ni0.5Cu0.5Fe2O4) supported on activated carbon (AC) (AC@Ni0.5Cu0.5Fe2O4) was synthesized and used as adsorbent, photocatalyst, and activator of peroxydisulfate (PDS) to realize the removal of ciprofloxacin (CIP). AC@Ni0.5Cu0.5Fe2O4 properties were characterized by scanning electron microscope equipped with energy-dispersive X-ray (SEM-EDX), X-ray diffraction (XRD), N2 adsorption-desorption isotherm plot of Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH), vibrating sample magnetometer (VSM). A rapid removal rate (94.30%) of CIP was achieved on AC@Ni0.5Cu0.5Fe2O4/PDS/UV system with the condition of catalyst dosage 0.30 g/L, initial pH 7.3, PDS addition 0.20 mM, CIP concentration 10 mg/L (200 mL), UV 28 W, in 30 min. Free radical quenching experiments indicate that reactive species of superoxide (·O2−), holes (h+), sulfate radicals (SO4−·) and hydroxyl radicals (·OH) were produced and all worked. The reusability test demonstrated that AC@Ni0.5Cu0.5Fe2O4 could be recycled five times with minimal performance reduction for the removal of CIP. The XRD and SEM of the after used AC@Ni0.5Cu0.5Fe2O4 did not change significantly, which further showed its stability and recyclability. This work might provide new insight into the application of AC@Ni0.5Cu0.5Fe2O4 in photocatalysis coupled with adsorption in peroxydisulfate assisted system and has high potential in CIP removal.
Bacteriophages Concept and Applications: A Review on Phage Therapy
Authors: Singh Kuldeep , Kumar Pradeep , Sahu Rasti , Singh Ankit Kumar , Kumar Adarsh 
Published: November 2022

Abstract:
The nature of phages was a matter of dispute, which was resolved in 1940, and it was
continued to develop their activity and application in the Soviet Union and Eastern Europe. Bacteriophages
were first employed in 1919 to treat bacterial illnesses caused by Citrobacter, Enterobacter,
and Pseudomonas.

:
Bacteriophages range in complexity from simple spherical viruses with genome sizes of less than 5 kbp to
complicated viruses with genome sizes surpassing 280 kbp. They have two significant parts, head and tail,
and are made up of numerous copies of more than 40 distinct proteins. Bacteriophages have been demonstrated
to bind with receptors in the walls of both gram-positive and gram-negative bacteria, ranging from
peptide sequences to polysaccharide moieties. Depending on the type of phage and the physiological state
of the bacterium, the life cycle may diverge into the lytic cycle or lysogenic cycle. Lytic-lysogenic switch
depends on a variety of inducing factors.

:
Bacteriophage therapy can be administered via several routes, but parenteral routes are the most effective.
Auto-dosing, single-dose potential, lack of cross-resistance with antibiotics, etc., are several advantages
of phage therapy over antibiotic treatment. Bacteriophages are attracting much attention because
of their potential advantages and wide applications as antibacterial agents, diagnostic technologies,
phage-based products, and biocontrol agents. They also have several applications in the food
industry, agriculture/crop, farm animal and bee protection, environmental, and biosensor development.
Recent Developments and Challenges in the Application of Fungal Laccase
for the Biodegradation of Textile Dye Pollutants
Authors: Magalhães Flávia F. , Pereira Ana F. , Cristóvão Raquel O. , Barros Rita A. M. , Faria Joaquim L. , Silva Cláudia G. , Freire Mara G. , Tavares Ana P. M. 
Published: November 2022

Abstract:
According to the European Environment Agency, the textile industry is responsible for 20%
of global water pollution due to dyeing and finishing products, thus facing severe environmental challenges.
It is essential to design more biocompatible and sustainable treatment processes capable of
removing dyes from industrial wastewater to fight this environmental hazard. Chemical industries must
change traditional chemical-based concepts to more environmentally friendly and greener processes to
remove pollutants, including dyes. Enzymatic bioremediation is a smart tool and a promising alternative
for environmental pollutant degradation. The use of enzymes in dye decolourization makes the
process a green and clean alternative to conventional chemical treatments. Moreover, enzymemediated
biocatalysis decreases the formation of toxic by-products compared to chemical reactions.
The most used enzyme for the decolourization of dyes is laccase. Laccase is a multicopper oxidase
found in diverse organisms such as fungi. It promotes the oxidation of phenolic compounds and has a
wide range of substrate specificity, making it a promising enzyme for removing different dyes used by
the textile industry, including recalcitrant aromatic dyes. The present article gives a comprehensive
revision of textile dye decolourization, its types, recent developments in laccase-mediated dye bioremediation
technologies, the mechanism of biocatalysis, and their limitations and challenges. Emphasis
on the chemical pathways of laccase reaction mechanisms for dye bioremediation processes is also
provided. In addition, a brief overview of textile industries and the respective traditional treatment processes
for textile wastewater is presented.
Eco-friendly Synthesis of 2-Amino-4H-Chromene Catalysed by HRSPLAE
and Anti-cancer Activity Studies
Authors: Tonape Vasant T. , Kamath Aravind D. , Kamanna Kantharaju 
Published: November 2022

Background:
Several types of catalysts have been cited in the literature. However, the current work showed that a multi-component reaction involving aldehydes, malononitrile, and resorcinol or α/β-naphthol could produce 2-amino-4H-chromene in a more environmentally friendly manner. The reaction is optimized by both stirring and microwave methods, but the reaction carried out under microwave irradiation is found to be faster with easy separation of the product with high yield and purity. The catalyst is analyzed for the presence of elemental composition using Flame Photometry (FP) and SEM-EDX. The synthesis of 2-amino-4H-chromenes is catalyzed by the new, green catalyst HRSPLAE (Water Extract of Hibiscus Rosa Sinensis plant dry leaves ash) within 3-5 min. The final product is analyzed by FT-IR, 1H-, 13C-NMR, and mass spectrometry techniques and the product obtained is free from the use of chromatographic separation with isolation and yield of 80–95%. Selected 2-amino-4H-chromene derivatives (4b and 4c) were screened for their anti-cancer and antimicrobial activity in vitro.

Methods:
The agro-waste sourced from Hibiscus rosa-sinensis plant dry leaves ash is utilized for the preparation of HRSPLAE catalyst, which is employed for the synthesis of 2-amino-4H-chromene derivatives under microwave irradiation.

Results:
2-Amino-4H-chromene derivatives were obtained from aromatic aldehyde, malononitrile, and resorcinol or α/β naphthol catalyzed by HRSPLAE. They were comprehensively evaluated using flame emission spectrometry, SEM, and EDX.

Conclusion:
HRSPLAE outperforms expensive catalysts. An efficient simpler workup without column chromatography for increased yield through a new unique green method for the synthesis of 2-amino-4H-chromene derivatives has been developed.
Personalized Medicine: Advanced Treatment Strategies to Revolutionize
Healthcare
Authors: Malviya Rishabha , Singh Arun Kumar , Verma Swati 
Published: November 2022

Abstract:
Personalized medicine (PM) is about developing an individualized approach to each
patient's illness. Understanding how a patient's genomic portfolio renders them prone to various
diseases may be enhanced by discovering genetic, epigenetic, and medical evidence. Medical
therapy that is safe and effective for specific individuals may be predicted using the PM approach,
which is a complete expansion of an older methodology (One-Size-Fits-All). Patient’s well-being
and longevity may improve and costs are reduced if PM is used. Using existing biomarkers and
early genome and epigenomic processes to better understand PM may lead to earlier diagnosis of
the disease, including carcinogenesis. A key focus of the PM technique is preventative medicine,
which emphasizes proactive actions rather than depending only on reactive ones. More intrusive
procedures may be avoided or postponed using this technique, resulting in a higher quality of life
and lower financial burdens for patients. End-of-life care costs are putting a strain on governmentfunded healthcare systems across the globe, notably in the United States. When used in
conjunction with present treatments, PM may help them work better and lessen the disadvantages
of just non-PM methods. Using genetic profiling, doctors may choose a drug based on a
participant's genetic profile that minimizes unwanted side effects and ensures a better result while
also being less costly than a 'trial-and-error' approach to sickness treatment. The less effective nonPM ('trial-and-error') strategy leads to drug toxicity, severe adverse effects, reactive treatment, and
misdiagnosis. PM and proactive therapeutic regimens should be used more often to save costs and
enhance overall well-being.
Examining Women’s Roles in the Publication of Medical Texts During The Sixteenth and Seventeenth Centuries
Authors: Johnston Katrina 
Published: November 2022

Many obstacles prevented women from fully participating in medical professions throughout Early Modern England. Women could not learn about medicine at formal institutes, including Oxford and Cambridge, since contemporary scholars believed that women were incapable of the abstract thinking necessary to practice the science. Throughout the sixteenth and seventeenth centuries, the Royal College of Physicians prosecuted female medical practitioners for what they deemed to be unsanctioned activity in the medical field. Writing and publishing medical texts was also a difficult profession for women to pursue. Although women’s ability to produce documents of this nature improved for a time as a consequence of the decrease in print censorship following the English Civil War (1642-1651), male-authored books published on the subject continued to question their knowledge publicly.
Despite these numerous obstacles, females did participate in medical publications. Women evaded the Royal College of Physicians’ sanctions and participated in the world of medical publications through disclosing their treatments to male-physician authors, publishing almanacs, and using metaphors to conceal the medical advice in their texts. In three sections, this article highlights various ways women were involved in the publication of medical texts. The first component will examine the gender dynamics of medical publishing, focusing on how male authors utilized women’s knowledge to help sell their own texts. The second two sections examine how women were involved in medical literature in their own right and the strategies they employed to participate in medicine while simultaneously avoiding public scrutiny.
Identification of viral RNA sequences in vampire bats (Desmodus rotundus) from central Mexico
Authors: Espinoza-Gómez Aldo , Moreno-Santillán Diana D. , Juárez-Maldonado Rafael , Gutiérrez Edgar G. , Salazar Ma. Isabel , Alonso-Palomares Luis A. , López-Vidal Juan Carlos , Elizalde-Arellano Cinthia , Ortega Jorge 
Published: November 2022

Bats are important reservoirs of viral entities that cause diverse economical and health problems in several sectors. The purpose of this study was to determine the prevalence of the rabies virus in vampire bats. We also tested for 3 other zoonotic viruses in the collected tissue. We captured 45 vampire bats (Desmodus rotundus), which were measured and sexed under standard parameters. We followed an RT-PCR reaction to amplify the viral products of four virus types: chikungunya (CHIKV), dengue (DENV), rabies (RABV) and Zika virus (ZIKV) from cerebral tissue. We obtained an amplicon of 100 bp for ZIKV in 2 samples, representing a prevalence of 4.4%. An amplicon of 581 bp for RABV was observed in 9 individuals, which is equivalent to 20% of our sample. We obtained 4 different haplotypes for RABV and a single haplotype for ZIKV. We did not record the presence of DENV and CHIKV. We corroborated the presence of the family Rhabdoviridae in the vampire bat and recorded the presence of ZIKV for the first time in this bat species.
Developing ESP based-digital learning materials support students’ needs at Indonesian vocational schools: Perceived quality
Authors: Fadlia Fadlia , Asra Surya , Zulida Evi , Santosa Made Hery 
Published: November 2022

The purpose of incorporating digital learning materials into vocational school English instruction is to assist students in acquiring specific competencies. Additionally, prepared digital learning materials are intended to assist teachers in developing instructional materials that follow the principles of English for Specific Purposes (ESP) in vocational high schools (SMK). The research used the qualitative research and development (R&D) method. The stages of developing this teaching material model are adapted from Thiagarajan, Semmel, and Semmel's 4Ds model (1974), which consists of four stages: define, design, develop, and disseminate. As data sources, the participants were two teachers from two different vocational schools with a combined enrolment of 115 students. This research's results are a model for developing digital-based teaching materials (prepared digital learning materials). Furthermore, it was discovered that using the prepared digital learning materials greatly aided teachers in teaching English to achieve specific competencies. From the students' perspective, they admitted that it was also exciting because they perceived learning as distinct from traditional learning materials due to digital materials usage.