bluuebunny/crossref_metadata_2025_split huggingface dataset:
IMPLEMENTASI KEBIJAKAN PROGRAM SI TETEH DALAM PELAYANAN PERIZINAN PADA DINAS PENANAMAN MODAL DAN PELAYANAN TERPADU SATU PINTU KABUPATEN KARAWANG
Authors: Sopiyah Siti , Argenti Gili , Nababan Rudyk 
Published: January 2022

Penelitian ini dilatarbelakangi karena masih adanya percetakan fisik dokumen yang dilakukan di Kantor Dinas Penanaman Modal dan Pelayanan Terpadu Satu Pintu (DPMPTSP) Kabupaten Karawang. DPMPTSP berkomitmen untuk memberikan pelayanan terbaik kepada masyarakat, seperti upaya penyingkatan waktu, prosedur, dan biaya pemberian perizinan dan nonperizinan, juga didukung Pelayanan Secara Elektronik (PSE). Hal tersebut diatur dalam Peraturan Bupati Karawang Nomor 40 Tahun 2017 tentang Pelayanan Perizinan Secara Elektronik dan Peraturan Bupati Karawang Nomor 50 Tahun 2018 tentang Perubahan Atas Peraturan Bupati Karawang Nomor 40 Tahun 2017 tentang Pelayanan Perizinan Secara Elektronik. Tujuan dari penelitian ini untuk mendeskripsikan bagaimana Implementasi Kebijakan Program Si Teteh Dalam Pelayanan Perizinan Pada Dinas Penanaman Modal dan Pelayanan Terpadu Satu Pintu Kabupaten Karawang. Penelitian ini menggunakan metode deskriptif dengan pendekatan kualitatif. Lalu teknik pengumpulan data menggunakan beberapa cara yaitu studi pustaka, observasi, wawancara, dan dokumentasi. Adapun teknik analisis data dilakukan melalui reduksi data, penyajian data dan juga penarikan kesimpulan dan verifikasi. Hasil penelitian ini menjelaskan bahwa implementasi kebijakan program Si Teteh dalam pelayanan perizinan ini belum efektif, karena berdasarkan teori implementasi kebijakan Charles O. Jones, (1994:166) yang terdiri dari tiga dimensi belum terpenuhi dengan baik, antara lain: 1) Organisasi, Sumber Daya Manusia terdiri dari ASN dan Non-ASN, fasilitasnya seperti komputer, printer dan jaringan internet; 2) Interpretasi, sosialisasi melalui media sosial, baliho, brosur, undangan perwakilan dari pemegang investasi, diundang media massa dan media online juga; dan 3) Penerapan, rapat evaluasi dilaksanakan setiap terdapat masalah atau peraturan baru dalam aplikasi Si Teteh.
EVALUASI ON GOING DANA ALOKASI KHUSUS FISIK BIDANG PENDIDIKAN DALAM MASA PANDEMI CORONA VIRUS DISEASE-19 BERBASIS INTELLIGENCE MEDIA MANAGEMENT (IMM)
Authors: Pambudi Andi Setyo 
Published: January 2022

Alokasi DAK bidang pendidikan selalu tersedia setiap tahun dengan jumlah anggaran yang besar dibandingkan bidang yang lain, bahkan di masa pandemi COVID-19. Pada level pemerintah daerah, bidang pendidikan adalah prioritas. Kebutuhan pendanaan pemerintah daerah melalui DAK Fisik diharapkan selaras dengan keinginan pemerintah pusat. Pelaksanaan DAK Fisik Bidang Pendidikan sebagai instrumen pendanaan ke daerah pada tahun 2020 menghadapi tantangan mewabahnya COVID-19 pada bulan Maret yang mempengaruhi optimalisasi proses pembangunan. Pemerintah mengambil opsi kebijakan relaksasi untuk mempermudah implementasi DAK di lapangan. Hal ini memerlukan evaluasi on-going dengan metode sesuai keadaan saat itu sebagai masukan untuk perencanaan tahun berikutnya. Implementasi DAK Fisik dan segala menu-menunya mendorong pentingnya informasi capaian maupun kendala lapangan tetap aktual serta dapat dipertanggungjawabkan secara ilmiah. Penelitian bertujuan menyajikan kendala, informasi dan hal-hal terkait pelaksanaan DAK Fisik Bidang Pendidikan tahun 2020 saat masa pandemi COVID-19 sebagai respons kondisi terkini tanpa harus melakukan kunjungan lapangan. Metode yang digunakan adalah studi literatur dan analisis statistik berbasis big data sebagai opsi yang relevan dengan situasi saat ini dalam rangka landasan memberikan rekomendasi perencanaan pembangunan. Analisis big data memanfaatkan tools Intelligence Media Management (IMM) untuk melihat persepsi publik terkait pelaksanaan DAK Fisik Bidang Pendidikan saat pandemi COVID-19. Hasil penelitian menunjukkan bahwa sebanyak 40 persen pemberitaan terkait DAK Bidang Pendidikan selama Pandemi COVID-19 berupa sentimen positif, 17 persen negatif dan 43 persen sentimen netral. Dalam sentimen negatif, pemberitaan yang disorot adalah tuntutan agar pengelola DAK dapat segera menyelesaikan pembangunan sesuai petunjuk teknis di lapangan. Top issue pemberitaan DAK Pendidikan sebagai masukan perbaikan pembangunan adalah tentang temuan-temuan ketidaksesuaian data pokok pendidikan (DAPODIK) dengan fakta di lapangan yang pada akhirnya berdampak pada kurang tepatnya sasaran penerima DAK.

Kata Kunci: Big Data, DAK, Evaluasi, Pendidikan
PERAN APARATUR DALAM MENGATASI PENULARAN PANDEMI COVID-19 DI KABUPATEN BANDUNG BARAT PROVINSI JAWA BARAT
Authors: Mulianingsih Sunasih 
Published: January 2022

Abstrak
Maksud dan tujuan dari penelitian ini adalah untuk mendiskripsikan dan mendapatkan data, sejauhmana peran aparatur Kabupaten Bandung Barat dalam menangani pendemi covid 19, Kabupaten Bandung Barat saat ini termasuk wilayah yang terdampak penyebaran dan penularan covid 19, dimana sebahagian Masyarakat dan jajaran aparatur terkonfimasi positif terkena covid 19. Sehingga peneliti ingin mengetahui sejauhmana peran aparatur Kabupaten Bandung Barat dalam mengatasi penularan pandemi covid 19. Penelitian ini menggunakan metode kualitatif diskriptif dengan pendekatan induktif, dan dapat diperoleh melalui data sekunder yang bersumber dari media cetak media elektronik dan dokumentasi-dokumentasi terkait dengan covid 19. Simpulan bahwa dalam implementasinya peran aparatur sangat besar dalam menangani msibah kesehatan ini, tim gugus tugas yang dibentuk oleh Bupati, mengawasi jalannya operasional sehari-hari. Hasil nya Kabupaten Bandung Barat dapat mengendalikan angka penularan dan yang sembuh semakin meningkat. Walaupun saat ini covid 19 belum melandai, tetapi khususnya di Kabupaten Bandung Barat dapat dikendalikan.

Kata Kunci : Peran, Aparatur, Penularan dan Covid 19.

Abstract
The purpose and objective of this study is to describe and obtain data, to what extent is the role of the West Bandung Regency apparatus in dealing with the COVID-19 pandemic, currently West Bandung Regency is an area affected by the spread and transmission of COVID-19, where some of the community and apparatus have been confirmed positive for COVID-19. 19. So that researchers want to know the extent of the role of the West Bandung Regency apparatus in overcoming the transmission of the covid 19 pandemic. This study uses descriptive qualitative methods with an inductive approach, and can be obtained through secondary data sourced from print media, electronic media and documentation related to covid 19. The conclusion is that in its implementation the role of the apparatus is very large in handling this health problem, the task force team formed by the Regent oversees the day-to-day operations. As a result, West Bandung Regency was able to control the number of infections and those who recovered were increasing. Even though at this time Covid 19 has not been sloping, but especially in West Bandung Regency, it can be controlled.

Keywords: Role, Apparatur, Transmission and Covid 19
ANALISIS JABATAN DALAM PENEMPATAN PEGAWAI DI BIRO PEMERINTAHAN DAN OTONOMI DAERAH SEKRETARIAT PROVINSI SULAWESI UTARA
Authors: Tamara Claudio V.D. , Johanes Ayu Widowati , Marlina Lina , Hendra Khansa Aulia 
Published: January 2022

Abstrak

Dalam penyelenggaraan manajemen sumber daya pegawai sektor publik, terdapat bahasan yang menarik untuk dikaji yaitu tentang penempatan pegawai berdasarkan kompetensinya sehingga dapat melakukan pekerjaaannya dengan efektif. Penelitian berfokus untuk untuk mengkaji dan menjelaskan analisis jabatan dalam menempatkan pegawai di Biro Pemerintahan Dan Otonomi Daerah, Sekretariat Provinsi Sulut. Penelitian ini menggunakan metode deskritif kualitatif. Data diperoleh dari objek penelitian yang terdiri dari para pejabat struktural, fungsional dan staf biro pemerintahan dan otonomi daerah pada pemerintah Provinsi Sulut. Analisis jabatan pada Biro Pemerintahan dan Otonomi Daerah di Pemerintah Provinsi Sulut telah dilaksanakan namun belum maksimal hal ini dapat dilihat masih banyak posisi jabatan yang kosong (belum terisi) dan ada beberapa ketidaksesuaian antara pendidikan pemangku jabatan dan klasifikasi jabatan.

Kata Kunci: Analisis Jabatan, Penempatan Pegawai, Pengembangan Kompetensi
Upaya Badan Kepegawaian Daerah Mengimplementasikan Kebijakan Disiplin ASN dalam Mengelola Keuangan Daerah di Kabupaten Agam
Authors: Dal Zaini Juniansyah 
Published: January 2022

Penelitian ini dilatarbelakangi oleh rendahnya tingkat disiplin Pegawai Negeri Sipil (PNS). Penelitian ini dengan tujuan guna meningkatkan pemahaman betapa berartinya ketertiban untuk Aparatur Sipil Negara selaku penyelenggaraan pemerintahan dalam rangka mewujudkan pemerintahan yang baik sebab tegak ataupun runtuhnya negara bergantung dari sumber daya manusianya ialah Aparatur Negara. Guna riset ini diharapkan bisa membagikan kontribusi dalam pengembangan pemberdayaan disiplin ASN selaku aparatur pemerintah, abdi negara serta abdi masyarakat jua bisa membagikan kontribusi pemikiran serta wacana dalam penyelenggaraan pemerintahan yang bersih serta berwibawa khususnya dalam perihal penerapan peraturan kepegawaian untuk ASN. Teknik pengumpulan data dilakukan melalui metode studi pustaka dan studi dokumen serta terjun langsung kelapangan dimana yang diteliti berupa aturan-aturan yang tertulis dalam undang-undang yang berlaku, dengan pengamatan secara langsung melalui wawancara terhadap responden. Hasil penelitian ini terbukti kalau masih adanya pelanggaran disiplin yang dilakukan oleh ASN yang mempengaruhi citra reformasi birokrasi dinegara Indonesia. Upaya untuk meningkatkan disiplin pegawai akan terus dilakukan sehingga ASN bisa lebih baik kedepannya.
The GDF11 Promotes Nerve Regeneration After Sciatic Nerve Injury in Adult Rats by Promoting Axon Growth and Inhibiting Neuronal Apoptosis
Authors: Lin Junhao , Shi Jie , Min Xiang , Chen Si , Zhao Yunpeng , Zhang Yuanqiang , Cheng Lei 
Published: January 2022

Introduction: Sciatic nerve injury is a common injury of the nervous system. Stem cell-based therapies, drug-based therapies and rehabilitation physiotherapy therapies are currently available, but their limited therapeutic efficacy limits their use. Here, we aimed to explore a novel lentiviral-based gene therapeutic strategy and to elaborate its mechanism.Materials and Methods: Recombinant GDF11 protein was used for the in vitro treatment of dorsal root ganglion (DRG) cells. Lentivirus was used to construct a vector system for the in vivo expression of GDF11. The nerve conduction function was detected using action-evoked potentials at different time periods, and the regulatory effect of nerves on target organs was detected by weighing the gastrocnemius muscle. Immunofluorescence of NF200 and S100 was used to show the regeneration of the sciatic nerve, and myelin and Nissl staining were performed to observe the pathological features of the tissue. Western was used to validate signaling pathways. The expression of related genes was observed by qPCR and Western blotting, and cell apoptosis was detected by flow cytometry.Result: GDF11 promotes the axonal growth of DRG cells and inhibits DGR cell apoptosis in vitro. GDF11 acts by activating the Smad pathway. GDF11 promotes the recovery of damaged sciatic nerve function in rats, the regeneration of damaged sciatic nerves in rats, and myelin regeneration of damaged sciatic nerves in rats. GDF11 also exerts a protective effect on neuronal cells in rats.Conclusion: Based on the present study, we conclude that GDF11 promotes axonal growth and inhibits DRG cell apoptosis in vitro through the Smad pathway, and lentivirus-mediated GDF11 overexpression in vivo can promote the recovery of sciatic nerves after transection by promoting axonal growth and inhibiting neuronal apoptosis in the spinal cord.
Isothiocyanate From Moringa oleifera Seeds Inhibits the Growth and Migration of Renal Cancer Cells by Regulating the PTP1B-dependent Src/Ras/Raf/ERK Signaling Pathway
Authors: Xie Jing , Qian Ying-Yan , Yang Yang , Peng Lin-Jie , Mao Jia-Ying , Yang Ming-Rong , Tian Yang , Sheng Jun 
Published: January 2022

Moringa oleifera Lam. is a tropical and subtropical plant that has been used for centuries as both food and traditional medicine. 4-[(α-L-Rhamnosyloxy) benzyl] isothiocyanate (MIC-1) is an active substance in M. oleifera, with anti-cancer activity. However, whether MIC-1 exerts anti-renal cancer effects is unknown. Therefore, the aim of the present study was to evaluate the effects of MIC-1 on the growth and migration of renal cell carcinoma (RCC) cells and to identify the putative underlying mechanism. We found that, among 30 types of cancer cells, MIC-1 exerted the strongest growth inhibitory effects against 786-O RCC cells. In addition, MIC-1 (10 μM) significantly inhibited the growth of five RCC cell lines, including 786-O, OSRC-2, 769-P, SK-NEP-1, and ACHN cells, but was not toxic to normal renal (HK2) cells. Also, MIC-1 suppressed 786-O and 769-P cell migration and invasion abilities, and reduced the expression of matrix metalloproteinase (MMP)-2 and MMP-9. Furthermore, MIC-1 induced apoptosis and cell cycle arrest, increased Bax/Bcl-2 ratio, and decreased cell cycle-related protein expression in 786-O cells and 769-P cells. Molecular docking and small-molecule interaction analyses with PTP1B both showed that MIC-1 inhibited PTP1B activity by binding to its active site through hydrogen bonding and hydrophobic interactions. Additionally, MIC-1 could suppress the growth and migration of 786-O cells by inhibiting PTP1B-mediated activation of the Src/Ras/Raf/ERK signaling pathway. In vivo experiments further showed that MIC-1 markedly inhibited the growth of xenograft tumors in mice, and greatly increased Bax/Bcl-2 ratio in tumor tissues. In addition, MIC-1 had no effect on the PTP1B-dependent Src/Ras/Raf/ERK signaling pathway in HCT-116 cells, Hep-G2 cells, and A431 cells. Overall, our data showed that MIC-1 could be a promising, non-toxic, natural dietary supplement for the prevention and treatment of renal cancer.
The Diagnostic Accuracy of One-Step Nucleic Acid Amplification for Lymph Node Metastases of Papillary Thyroid Carcinoma： A Systematic Review and Meta-Analysis
Authors: Wang Xiaofei , Zheng Xun , Zhu Jingqiang , Li Zhihui , Wei Tao 
Published: January 2022

BackgroundOne-step nucleic acid amplification (OSNA) analysis is a molecular diagnostic technique for lymph node metastases (LNMs) by quantifying cytokeratin 19(CK 19) mRNA. We aim to evaluate the intraoperative diagnostic accuracy of OSNA assay for LNMs of papillary thyroid carcinoma (PTC).MethodsPubMed, Embase, Cochrane Library, and Web of Science databases were searched to retrieve related literature. A meta-analysis was performed using STATA11.0, Meta-Disc 1.4 and RevMan 5.3.ResultsThis meta-analysis included six studies involving 987 lymph nodes from 194 patients. The pooled sensitivity, specificity, and area under the summary receiver-operating characteristic curve (AUC) of OSNA for detecting LNM were 0.88, 0.90, and 0.95, respectively.ConclusionOSNA assay is an accurate molecular diagnosis for intraoperative detection of lymph node metastasis in PTC.
Metabolic Syndrome and Its Components Are Associated With Altered Amino Acid Profile in Chinese Han Population
Authors: Sun Shuiya , He Dongjuan , Luo Cheng , Lin Xihua , Wu Jiahua , Yin Xueyao , Jia Chengfang , Pan Qianqian , Dong Xuehong , Zheng Fenping , Li Hong , Zhou Jiaqiang 
Published: January 2022

ObjectiveRecent studies have found that the levels of plasma amino acids, such as branched-chain amino acids and aromatic amino acids, were associated with visceral obesity, insulin resistance, future development of diabetes and cardiovascular diseases. However, few studies have involved a Chinese Han population. This study aimed to examine the association between amino acid profile and metabolic syndrome (MetS) and its components in the Chinese Han population.MethodsThis is a cross-sectional study, which enrolled a cohort of 473 participants from a community. We employed the isotope internal standard method to determine the plasma concentrations of 28 amino acids using high-performance liquid chromatography-tandem mass spectrometry (LC/MS). Participants were divided into MetS (n = 72) and non-MetS groups (n = 401) to analyze the association between amino acids and MetS and its components.ResultsThe prevalence of MetS was 15.2% according to the criteria. Plasma concentrations of isoleucine (Ile), leucine (Leu), valine (Val), tyrosine (Tyr), tryptophan (Trp), phenylalanine (Phe), glutamic acid (Glu), aspartic acid (Asp), alanine (Ala), histidine (His), methionine (Met), asparagine (Asn), and proline (Pro) were significantly higher in the MetS group than those in the non-MetS group (P &lt; 0.05), but taurine (Tau) was significantly lower (P &lt; 0.05). When MetS components were increased, the concentrations of these 13 amino acids significantly increased (P &lt; 0.05), but Tau concentration was significantly decreased (P &lt; 0.05). We extracted the amino acid profile by principal component analysis (PCA), PC1 and PC2, which extracted from the 14 amino acids, were significantly associated with MetS (odds ratio, 95% confidence interval: 1.723, 1.325–2.085 and 1.325, 1.043–1.684, respectively). A total of 260 non-MetS participants were followed up effectively, and 42 participants developed new-onset MetS within 5 years. We found that the amino acid profile of PC1 was linked to the occurrence of future MetS. Decreased Tau was correlated with the future development of MetS.ConclusionParticipants with MetS exhibit an abnormal amino acid profile, and its components gradually increase when these amino acids are altered. Amino acid PCA profile can be employed for assessing and monitoring MetS risk. Finally, decreased Tau may be linked to the future development of MetS.
Bioinformatic Analysis Combined With Experimental Validation Reveals Novel Hub Genes and Pathways Associated With Focal Segmental Glomerulosclerosis
Authors: Hou Yan-Pei , Diao Tian-Tian , Xu Zhi-Hui , Mao Xin-Yue , Wang Chang , Li Bing 
Published: January 2022

Background: Focal segmental glomerulosclerosis (FSGS) is a type of nephrotic syndrome leading to end-stage renal disease, and this study aimed to explore the hub genes and pathways associated with FSGS to identify potential diagnostic and therapeutic targets.Methods: We downloaded the microarray datasets GSE121233 and GSE129973 from the Gene Expression Omnibus (GEO) database. The datasets comprise 25 FSGS samples and 25 normal samples. The differential expression genes (DEGs) were identified using the R package “limma”. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using the database for Annotation, Visualization and Integrated Discovery (DAVID) to identify the pathways and functional annotation of the DEGs. The protein–protein interaction (PPI) was constructed based on the Search Tool for the Retrieval of Interacting Genes (STRING) database and visualized using Cytoscape software. The hub genes of the DEGs were then evaluated using the cytoHubba plugin of Cytoscape. The expression of the hub genes was validated by quantitative real-time polymerase chain reaction (qRT-PCR) using the FSGS rat model, and receiver operating characteristic (ROC) curve analysis was performed to validate the accuracy of these hub genes.Results: A total of 45 DEGs including 18 upregulated and 27 downregulated DEGs, were identified in the two GSE datasets (GSE121233 and GSE129973). Among them, five hub genes with a high degree of connectivity were selected. From the PPI network, of the top five hub genes, FN1 was upregulated, while ALB, EGF, TTR, and KNG1 were downregulated. The qRT-PCR analysis of FSGS rats confirmed that the expression of FN1 was upregulated and that of EGF and TTR was downregulated. The ROC analysis indicated that FN1, EGF, and TTR showed considerable diagnostic efficiency for FSGS.Conclusion: Three novel FSGS-specific genes were identified through bioinformatic analysis combined with experimental validation, which may promote our understanding of the molecular underpinning of FSGS and provide potential therapeutic targets for the clinical management.
Development of Inclusion Complexes With Relative Humidity Responsive Capacity as Novel Antifungal Agents for Active Food Packaging
Authors: Muñoz-Shugulí Cristina , Rodríguez-Mercado Francisco , Mascayano Carolina , Herrera Andrea , Bruna Julio E. , Guarda Abel , Galotto María J. 
Published: January 2022

Background:Allyl isothiocyanate is an excellent antimicrobial compound that has been applied in the development of active food packaging materials in the last years. However, the high volatility of this compound could prevent a lasting effect over time. In order to avoid this problem, cyclodextrin inclusion complexes have been proposed as an alternative, being beta-cyclodextrin (β-CD) as the main candidate. In addition, β-CD could act as a relative humidity-responsive nanoparticle. In this regard, the aim of this study was to develop inclusion complexes based on β-CD and AITC as relative humidity-responsive agents, which can be used in the design of active food packaging materials.Methods:Two different β-CD:AITC inclusion complexes (2:1 and 1:1 molar ratios) were obtained by the co-precipitation method. Entrapment efficiency was determined by gas chromatography, while inclusion complexes were characterized through thermal, structural, and physicochemical techniques. Antifungal capacity of inclusion complexes was determined in a headspace system. Furthermore, the AITC release from inclusion complexes to headspace at different percentages of relative humidity was evaluated by gas chromatography, and this behavior was related with molecular dynamic studies.Key Findings and Conclusions:The entrapment efficiency of inclusion complexes was over to 60%. Two coexisting structures were proposed for inclusion complexes through spectroscopic analyses and molecular dynamic simulation. The water sorption capacity of inclusion complexes depended on relative humidity, and they exhibited a strong fungicide activity againstBotrytis cinerea. Furthermore, the AITC release to headspace occurred in three stages, which were related with changes in β-CD conformational structure by water sorption and the presence of the different coexisting structures. In addition, a strong influence of relative humidity on AITC release was evidenced. These findings demonstrate that β-CD:AITC inclusion complexes could be used as potential antifungal agents for the design of food packaging materials, whose activity would be able to respond to relative humidity changes.