bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging
Authors: Xu Yingna , Xu Xing , Zhang Yong , Qian Jin 
Published: October 2022

Abstract
Circular RNAs (circRNAs) are important non-coding RNAs (ncRNAs) involved in the development of multiple human diseases, especially cancers. circRNA_0084043 is significantly involved in the progression of melanoma. However, whether circRNA_0084043 is associated with glioma remains unknown. In this study, the upregulation of circRNA_0084043 in glioma and the association between circRNA_0084043 and glioma grade were identified. Our results showed that circRNA_0084043 is significantly involved in the proliferative, migratory, and invasive capacities of glioma cells. The results obtained from starBase, luciferase reporter assays, RNA immunoprecipitation assays, and RNA pull-down assays demonstrated that circRNA_0084043 acts as a direct sponge for miR-577. To identify the potential target of miR-577, analysis was conducted using the TargetScan algorithm, and results showed that SNX5 is a candidate miRNA for miR-577 binding. Finally, glioma cell growth, migration, and invasion were significantly enhanced by circRNA_0084043 by regulating the expression of miR-577-mediated SNX5 expression via miR-577 sponging. Taken together, we concluded that circRNA_0084043 in the miR-577/SNX5 axis can be used as a candidate target for glioma therapy.
Induction of apoptosis via inactivating PI3K/AKT pathway in colorectal cancer cells with the aged Hakka stir-fried green tea
Authors: Zhang Xin-yue , Huang Hai-ying , Sun Shi-li , Li Dong-li , Sun Ling-li , Li Qiu-hua , Chen Ruo-hong , Lai Xing-fei , Zhang Zhen-biao , Zheng Xi , Wong Wing-Leung , Wen Shuai 
Published: October 2022

Abstract
Human colorectal cancer is notorious for its high morbidity and mortality; however, the lack of effective and low-toxic drugs has currently been a bottleneck for the treatment of the disease. The present study reported a new functional food of Hakka stir-fried green tea (HSGT) aged with different years, including those stored starting from 2003 (03Y), 2007 (07Y), 2011 (11Y), 2015 (15Y) and 2019 (19Y) respectively, for their effective therapeutic activity against colorectal cancer. The major active ingredients including polyphenols, catechins, amino acids, catechins, gallic acid and caffeine found in the lyophilized powder of these aged HSGT were analyzed with high performance liquid chromatography. Our results showed that, at the cellular level, all these aged HSGT inhibited significantly the proliferation of colon cancer cells (HT-29) in a concentration-dependent manner. In particular, the batch of 15Y and 19Y exhibited the highest inhibition rate for 48 hours treatment. Further, all these aged HSGT examined were able to enhance the apoptosis of HT-29 cells and block the transition of G1/S phase population to G2/M phase. Western blotting results also showed that the aged HSGT inhibited CDK2, CDK4 and CylinB1 protein expression, as well as increased PRAP expression and Bax/Bcl2 ratio in HT-29 cells. In addition, an upstream signal, PI3K/AKT signaling, was found involving in this regulation, as evidenced by the inhibition of phosphorylated PI3K and AKT by the aged HSGT. Therefore, our study reveals that the aged HSGT may inhibit colon cancer cell proliferation, cell cycle progression and promoted apoptosis of colon cancer cells by inactivating PI3K/AKT signalling. The therapeutic effects of the HSGT aged with different years were also investigated.
Characterization of rumen microbiome and metabolome from oro-esophageal tubing and rumen cannula in Holstein dairy cows
Authors: Cunha Lais L. , Monteiro Hugo F. , Figueiredo Caio C. , Canisso Igor F. , Bicalho Rodrigo C. , Cardoso Felipe C. , Weimer Bart C. , Lima Fabio S. 
Published: October 2022

Abstract
Less invasive rumen sampling methods such as oro-esophageal tubing became widely popular to explore the rumen microbiome and metabolome. However, it remains unclear if such methods represent well the rumen contents from rumen cannula technique. Herein, we characterized the microbiome and metabolome in the rumen content collected by an oro-esophageal tube and by rumen cannula in ten multiparous lactating Holstein cows. The 16SrRNA gene was amplified and sequenced using the IlluminaMiSeq platform. Untargeted metabolome was characterized using gas chromatography of a time-of-flight mass spectrometer. Bacteroidetes, Firmicutes, and Proteobacteriawere the top three most abundant phyla representing ~90% of all samples. Although the pH of oro-esophageal samples was greater than rumen cannula, we found no difference in alpha and beta-diversity among their microbiomes. The overall metabolome of oro-esophageal samples was slightly different from rumen cannula samples yet more closely related to the rumen cannula content as a whole, including its fluid and particulate fractions. Enrichment pathway analysis revealed a few differences between sampling methods, such as when evaluating unsaturated fatty acid pathways in the rumen. The results of the current study suggest that oro-esophageal sampling can be a proxy to screen the 16S rRNA rumen microbiome compared to the rumen cannula technique. The variation introduced by the 16S rRNA methodology may be mitigated by oro-esophageal sampling and the possibility of increasing experimental units for a more consistent representation of the overall microbial population. Studies should consider an under or over-representation of metabolites and specific metabolic pathways depending on the sampling method.
Assessment of Chemotherapy Response With Simple Biochemistry on Lymphoma Patients
Authors: Erdat Efe Cem 
Published: October 2022

Abstract
Background
Response to treatment is evaluated with radiologic and/or nuclear medicine studies in addition to a complete physical examination. Biochemical investigations have been used for many years in risk and prognostic stratification of lymphoma, but there is no clinically useful method to assess response to treatment in lymphoma. In our study, we investigated the suitability of routine biochemical assays for assessing response to treatment in lymphoma.
Patients and Methods:
We retrospectively collected 108 patients’ routine biochemical examinations and examined the changes in biochemical parameters in terms of good or poor response. Logistic regression modelling was done on biochemical parameters and the results were used for development an ordinal scoring system.
Results
Most of the changes in biochemical parameters before and after treatment were significant (p < 0.05). The levels of total protein, beta-2-microglobulin, lactate dehydrogenase and C-reactive protein and erythrocyte sedimentation rate had decreased in the patients with good response to treatment, while no change was observed in the patients with poor response to treatment. Cross-interaction between pre- and post-treatment beta-2-microglobulin, lactate dehydrogenase and total protein levels was significant (p = 0.02–0.03). We developed a 4-point ordinal scoring system for lymphoma response evaluation using biochemical parameters. Logistic regression showed significance (p < 0.01) and effect size was close to high effect (Cramer’s V test 0.46)
Conclusion
A 4-point ordinal scoring system for lymphoma response evaluation is proposed.
Efficacy of potassium-competitive acid blockers vs. proton pump inhibitors on gastric ulcers: Bayesian and frequentist network meta-analysis with cross-inference through a quality management system
Authors: Oh Hojin , Kim Kwang-Hyun , Lee Kwan-Haeng , Yoo Duk-Woo , Yoon In Mo 
Published: October 2022

Abstract
Proton pump inhibitors (PPIs) have been the mainstay of treatment for gastric ulcer (GU) for over 30 years. However, since the discovery of a new class of acid suppressants, potassium-competitive acid blockers (P-CABs), the desire for a therapeutic agent has continued and the clinical trials on P-CABs have been conducted. In our study, a systematic review and network meta-analysis (NMA) were performed based on randomized controlled trials (RCTs) conducted since the development of P-CABs. In addition, the new methodology of the inference concept was applied to confirm the results. Our quality management system was also integrated throughout the research to guarantee the accuracy of the data. Initially, we screened 431 studies and extracted 10 homogeneous GU RCTs with 6315 participants. The odds ratios (ORs) for the 4-week cure rate in Bayesian + frequentist NMA, tegoprazan 100 mg (OR = 4.14, 95% credible interval [CI] 0.56–26.3) and pantoprazole 40 mg (OR = 4.12, 95% confidence interval [CI] 1.90–8.88) were the largest, respectively. The ORs for the 8-week cure rate in Bayesian + frequentist NMA, lansoprazole 30 mg (OR = 8.77, 95% [CI] 0.95–78.9) and lansoprazole 30 mg (OR = 7.91, 95% [CI] 2.60–24.03) was the largest, respectively. As the inference by grouping PPIs and P-CABs, the results show similar trends in terms of effectiveness between two therapeutic classes. In conclusion, our study shows that the cure rates of P-CABs in cases of GU are not inferior to those of PPIs.
Exosomal miR-224-3p promotes lymphangiogenesis and lymph node metastasis by targeting GSK3B in gastric cancer
Authors: Ba Yi , He Yi , Zhou Zhengyang , Qiao Lei , Duan Jingjing , Zhang Haiyang 
Published: October 2022

Abstract
Patients with gastric cancer (GC) are prone to lymph node metastasis (LNM), which is an important factor for recurrence and poor prognosis of GC. Nowadays, more and more studies have confirmed that exosomes can participate in tumor lymphangiogenesis. In-depth exploration of the pathological mechanism in the process of LNM in GC may provide effective targets and improve the diagnosis and treatment effect. We used sequencing analysis of collected serum to screen out exo-miRNA related to LNM in GC. In this study, we screened out miR-224-3p related to GC progression and LNM in a vascular endothelial growth factor C (VEGFC)-independent manner. We found that exo-miR-224-3p derived from GC cells could enter human lymphatic endothelial cells (HLECs) and promote the tube formation and migration of HLECs. In addition, it was revealed that miR-224-3p could bind to the 3'UTR region of GSK3B mRNA. Then, we proved that inhibiting the expression of GSK3B could suppress the phosphorylation of β-catenin and promote the transcription of PROX1, thus leading to tumor lymphangiogenesis. Furthermore, it was also found that hnRNPA1 mediated the sorting of miR-224-3p into exosomes, and the high expression of PKM2 promoted the secretion of exo-miR-224-3p. Our discovery of the exo-miR-224-3p/GSK3B/β-catenin/PROX1 axis may provide a new direction for the clinical treatment of GC.
Nanoparticles Based Antimicrobial Susceptibility and Molecular Characterization of Extended Spectrum Beta-lactamase (ESBL) Producing Pathogens
Authors: Aftab Hamna 
Published: October 2022

Abstract
Antibiotic resistance is a global challenge that’s correlated with the increasing illness and mortality rates, so advanced antibacterial agents should be produced. Most of the urinary tract infections are due to the antibiotic resistance. Microbes develop defense because of misuse & excessive use of drugs. Those microbes produce extended spectrum beta lactamases (ESBL), plasmid encoded hydrolytic enzymes. They confer resistance to β antibiotics.Nanotechnology brought huge advancement for the UTIs treatment based upon the usage of metal nanoparticles such as silver NPs. Nanoparticles are the nano-scaled materials(under 100nm). Nanoparticles consists of wide range of antibacterial characteristics against the negative & positive bacteria, and could resolve the issue of microbial drug resistance. NPs can be used as an alternative to antibiotics to treat UTI. Herein, we utilized Moringa oleifera leaves extract to synthesise the silver nanoparticles (Ag-NPs) biogenically. The characterization of Ag-NPs was executed by UV-spectroscopy, scanning electron microscopy (SEM), FTIR, and X-ray diffraction XRD.The antibacterial activity studied against the bacterial isolates & UTI strains. We used the well diffusion method to evaluate the antibacterial activity Ag-NPs, we utilized 1ml of DMSO in which 40ml of Ag-NPs were added Serial dilutions were 1000µl, 500µl, 250µl and 125µl. The bacteria that were most responsive to it were gram positive Staphylococcus aeruginosa and to some extent gram negative Escherichia coli bacteria. The zones of inhibition were measured by employing the common ruler.
Heterogeneous Graph-Neural-Network with TimeSequence Information Integration
Authors: Goyal North , Choi Sagar , Rey Justin , Hill Joseph 
Published: October 2022

Abstract
Recommendation systems, as a kind of information filtering system, can understand users' interests based on their personal data or historical behavior records, and are widely used in Web applications such as e-commerce, search, and streaming websites. Based on the fact that users with similar preferences may be interested in similar items, most existing recommendation methods mine this synergistic information through user-item interactions, called collaborative filtering. Recent interaction information can better reflect users' dynamic interests over time. Another group of work is called temporal-based recommendation, which takes into account temporal information to model the user's dynamic interests. A temporal recommendation system is based on the user's temporal interaction data as a context to predict which item the user is most likely to interact with next time. Markov chain-based recommendation models are a typical example of temporal sequential recommendation, which assume that the next interaction is only related to the previous one. Recently, much work has used recurrent neural networks to model previous behaviors and use hidden states to predict the next behavior. However, almost all temporal recommendation methods model user embeddings based only on their own temporal interaction history, ignoring the heterogeneous information widely available in recommender systems, such as product attributes, and encounter cold-start problems when data are sparse and users have fewer interactions. In this paper we proposed a model, the heterogeneous dynamic graph neural network. This network uses a static graph encoder to process the node representation of each heterogeneous graph at different time steps, which includes the node attribute information and edge information, to capture the changing node information in all time steps of the dynamic graph and obtain a valid node representation at each time step. The long and short-term memory model is then used to aggregate the temporal information between different time steps and to mine the connections and interdependencies of different types of nodes between different time steps. The goal of the heterogeneous dynamic graph neural network is to capture the changing node representations of the same nodes as well as different nodes at different time steps in the graph network, expecting to better perform the task of node classification in dynamic graphs. In this paper, we validate the model of heterogeneous dynamic graph neural networks from data, experimenting with node classification tasks on real graph structured data including social networks, e-commerce networks and business review networks, and show that heterogeneous dynamic graph neural networks outperform the latest representation learning methods for static and dynamic graphs.
Draft genomes of three closely related low light-adapted Prochlorococcus
Authors: Berta-Thompson Jessie W , Thomas Elaina , Cubillos-Ruiz Andrés , Hackl Thomas , Becker Jamie W. , Coe Allison , Biller Steven J. , Berube Paul M. , Chisholm Sallie W. 
Published: October 2022

Abstract
Objectives:
The marine cyanobacterium Prochlorococcus is a critical part of warm ocean ecosystems and a model for studying microbial evolution and ecology. To expand the representation of this organism’s vast wild diversity in sequence collections, we performed a set of isolation efforts targeting low light-adapted Prochlorococcus. Three genomes resulting from this larger body of work are described here.
Data description:
We present draft-quality Prochlorococcusgenomes from enrichment cultures P1344, P1361, and P1363, sampled in the North Pacific. The genomes were built from Illumina paired reads assembled de novo. Supporting datasets of raw reads, assessments, and sequences from co-enriched heterotrophic marine bacteria are also provided. These three genomes represent members of the low light-adapted LLIV Prochlorococcusclade that are closely related, with 99.9% average nucleotide identity between pairs, yet vary in gene content. Expanding the powerful toolkit of Prochlorococcus genomes, these sequences provide an opportunity to study fine-scale variation and microevolutionary processes.
Pig adipose tissue of two different breeds and locations: morphology and Raman studies
Authors: Pchelkina Viktoriya , Chernukha Irina , Nikitina Marina , Ilin Nikolai 
Published: October 2022

According to the recent data, there are 4–5-local pig breeds left in Russia by now. Livni is among them. This breed is characterized by high fat content. Back fat has been analyzed earlier. We aimed to assess fat morphometrics from other localizations in pigs.
Sacral, axillary, and perirenal fat samples from 6-month-old Duroc and Livni pig breeds were analyzed using morphological and Raman-based techniques.
Livni adipocytes were characterized by dense packing with a polyhedron-like structure. In Duroc fat, they were more rounded (spherical). A “two-phase” cell disperse was identified in all samples. Fat cells in Livni pigs were bigger than those in the Duroc breed: 70–102, 15–18, and 26% for sacral, axillary, and perirenal locations. Differences in the intensity of the Raman signal between the samples were found: in the samples of subcutaneous adipose tissue, more intense peaks were observed, which are responsible for unsaturation; the samples of Livni axillary fat were characterized by greater unsaturation than sacral fat.
Livni and Duroc adipocytes differ from each other in form and size and the difference depends on location. Pork fat from local breeds is expected to have potentially more health protecting (for animals) and health promoting (for consumers) properties.
PLA2R Staining is Useful for the Diagnosis and Treatment of Membranous Nephropathy in Pediatric Patients
Authors: Tamura Hiroshi , Furuie Keishiro , Kuraoka Shohei , Kawano Tomoyasu , Nakazato Hitoshi 
Published: October 2022

Background:
Idiopathic membranous nephropathy (IMN) is a rare disease in children. The incidence is 1.5% in children with nephrotic syndrome. A few studies have also investigated the M-type phospholipase A2 receptor’s (PLA2R) potential role in pediatric IMN, reporting a low positive rate in pediatric kidneys.

Objective:
Therefore, we conducted immunofluorescence staining using an anti-PLA2R antibody in the renal biopsy specimens of eight pediatric patients with IMN.

Methods:
We studied the glomerular expression of PLA2R using tissues from children with IMN, and searched for papers on PLA2R staining in pediatric IMN on PubMed.

Results and Discussion:
Clinical characteristics of patients diagnosed with IMN in this study and the other three studies: A total of 20 pediatric (aged 2–12 years; mean age 7.4 ± 2.8 years) patients and 25 adolescent (aged 13–19 years; mean age 15.9 ± 2.0 years) patients, comprising 25 male (55.6%) and 20 female (44.4%) patients, with 23 (51.1%) patients with IMN being PLA2R-positive, were found to be eligible for this study. Furthermore, we found three papers through our online search.

Conclusion:
PLA2R expression can be approximately half positive in children with IMN, and it is useful to investigate the causative antigen of PLA2R in children.
The intensity of anti-PLA2R antibody expression reflected the disease activity (urinary protein level) of the patients in this study.
It is possible to adjust the drug dose in immunosuppressive therapy with reference to the expression intensity of PLA2R.
Pathogenetic Role of Disorders of Insulin Signaling Pathways and Diabetes-Associated Hyperglycemia in the Mechanisms of Diabetic Encephalopathy Formation and Hypersensitivity of the Brain to Ischemia-Reperfusion
Authors: Tkachuk Svitlana , Tkachuk Oleksiy , Nika Olha , Hodovanets Yuliya 
Published: October 2022

The aim of the study was to analyze the literature data regarding the pathogenetic role of insulin signaling disorders and diabetes-associated hyperglycemia in the mechanisms of diabetic encephalopathy and hypersensitivity of the brain to ischemia-reperfusion.
Conclusion. Analysis of the literature data shows a number of common links between the pathogenesis of diabetic encephalopathy and ischemic-reperfusion brain injury, which may, to some extent, explain the predisposition of diabetics to acute cerebral circulatory disorders and their adverse course. However, it is clear that the severity of such comorbid pathology cannot be explained only by the additive effect of individual links in the pathogenesis, which indicates the necessity for further in-depth study of its molecular-genetic aspects.
Integrating Human-Centered Design Methods Into a Health Promotion Project: Supplemental Nutrition Assistance Program Education Case Study for Intervention Design
Authors: Chen Elizabeth , Bishop Jared , Guge Cozon Lindsay , Hernandez Eduardo , Sadeghzadeh Claire , Bradley Megan , Dearth-Wesley Tracy , De Marco Molly 
Published: October 2022

Background
Human-centered design, or design thinking, offers an extensive toolkit of methods and strategies for user-centered engagement that lends itself well to intervention development and implementation. These methods can be applied to the fields of public health and medicine to design interventions that may be more feasible and viable in real-world contexts than those developed with different methods.

Objective
The design team aimed to develop approaches to building food skills among caregivers of children aged 0-5 years who are eligible for a federal food assistance program while they were in the grocery store.

Methods
They applied 3 specific human-centered design methods—Extremes and Mainstreams, Journey Mapping, and Co-Creation Sessions—to collaboratively develop intervention approaches to enhance Supplemental Nutrition Assistance Program Education (SNAP-Ed) reach and impact across food retail settings. Extremes and Mainstreams is a specific kind of purposive sampling that selects individuals based on characteristics beyond demographics. Journey Mapping is a visual tool that asks individuals to identify key moments and decision points during an experience. Co-Creation Sessions are choreographed opportunities for individuals to explicitly contribute to the design of a solution alongside research or design team members.

Results
Ten caregivers with diverse lived experiences were selected to participate in remote design thinking workshops and create individual journey maps to depict their grocery store experiences. Common happy points and pain points were identified. Nine stakeholders, including caregivers, SNAP-Ed staff, and grocery store dieticians, cocreated 2 potential intervention approaches informed by caregivers’ experiences and needs: a rewards program and a meal box option.

Conclusions
These 3 human-centered design methods led to a meaningful co-design process where proposed interventions aligned with caregivers’ wants and needs. This case study provides other public health practitioners with specific examples of how to use these methods in program development and stakeholder engagement as well as lessons learned when adapting these methods to remote settings.