bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation and Limitations of Different Approaches Among COVID-19 Fatal Cases Using Whole-exome Sequencing Data
Authors: Forgacova Natalia , Holesova Zuzana , Hekel Rastislav , Sedlackova Tatiana , Pos Zuzana , Krivosikova Lucia , Janega Pavol , Kuracinova Kristina Mikus , Babal Pavel , Radvak Peter , Radvanszky Jan , Gazdarica Juraj , Budis Jaroslav , Szemes Tomas 
Published: March 2022

Abstract
Background: COVID-19 caused by SARS-CoV-2 infection may result in various disease symptoms and severity, ranging from asymptomatic, through mild, up to very severe and fatal cases. Although environmental, clinical, and social factors play important roles in both susceptibility to SARS-CoV-2 infection and COVID-19 disease progress, it is becoming evident that both pathogen and host genetic factors are important too. Here we report whole-exome sequencing (WES) findings of 27 individuals who died as a result of COVID-19 infection, especially focusing on frequencies of DNA variants in genes previously associated with SARS-CoV-2 infection and COVID-19 severity. Results: We selected risk DNA variants/alleles or target genes using four different approaches: 1) aggregated GWAS results from the GWAS Catalog; 2) selected publications from PubMed; 3) the aggregated results of the Host Genetics Initiative database; and 4) a commercial DNA variant annotation/interpretation tool providing its own knowledgebase. We divided these variants/genes into those reported to influence the susceptibility to SARS-CoV-2 infection and those influencing COVID-19 severity. Based on these, we compared frequencies of alleles among the fatal COVID-19 cases to frequencies identified in two population control datasets (non-Finnish European population from the gnomAD database and genomic frequencies specific for the Slovak population from our own database). Our comparisons delineated a trend of higher frequencies of severe COVID-19 associated risk alleles among fatal COVID-19 cases, when compared to both control population datasets. This trend reached statistical significance specifically when using the HGI derived variant list. We also analyzed other approaches to WES data evaluation, where we showed their usage as well as limitations. Conclusions: Although our results proved the likely involvement of host genetic factors pinned out by previous studies for COVID-19 disease severity, careful considerations about the molecular-testing strategies and the evaluated genomic positions may have a strong impact on the utility of genomic testing.
TMBIM1 promotes EMT by stimulating autophagic degradation of E-cadherin via AMPK/mTOR/ULK1 axis in human gliomas
Authors: Gao Lun , Liu Junhui , Zhang Shenqi , Li Yong , Tan Yinqiu , Yang Ji’an , Cai Jiayang , Wang Long , Yuan Fan’en , Ye Zhang , Tong Shi’ao , Liu Baohui , Tian Daofeng , Deng Gang , Chen Qianxue 
Published: March 2022

Abstract
Background Gliomas are the most common malignant tumors of the central nervous system in adults, glioblastoma is notorious for its highly metastatic and recurrent, accounting for approximately 50% of all gliomas. Exploring its molecular mechanism is urgently needed for the treatment and prognosis evaluation of gliomas. Transmembrane BAX inhibitor motif-containing 1 (TMBIM1) has been reported to be associated with non-alcoholic steatohepatitis, tumor and other diseases. However, the role of TMBIM1 in GBM and the underlying mechanisms remains unclear.MethodsThe expression level and prognostic value of TMBIM1 in gliomas were investigated by public datasets, and further confirmed by western blot and immunohistochemistry (IHC) in our tissues. Intracranial xenograft model, IHC and western blot were used to evaluate the functional role of TMBIM1.ResultsTMBIM1 was over-expressed in GBM, and its high expression reduced the survival time of glioma patients. TMBIM1 induced EMT and autophagy, and inhibition of autophagy reverses TMBIM1-regulated EMT in vitro and in vivo. Intracranial xenograft model showed the survival time of mice in TMBIM1 knockdown group treated with chloroquine (CQ)was significantly prolonged. The loss of E-cadherin expression is considered the foundation of EMT, and we subsequently demonstrated that TMBIM1 stimulating autophagic degradation of E-cadherin via AMPK/mTOR/ULK1 axis. ConclusionOur study provides a novel mechanism for the regulation of EMT in the process of gliomas metastasis, indicating that inhibition of TMBIM1 activity to attenuate autophagy may be a potential strategy for the treatment of gliomas.
Length doesn´t play a role – Sexual Activity in Men After Short Stem Total Hip Arthroplasty
Authors: Navas Luis , Hauschild Matthias , Miehlke Wolfgang , Schmidt Sebastian , Streit Marcus , Kinkel Stefan , Zimmerer Alexander 
Published: March 2022

Abstract
BackgroundChronic hip pain due to osteoarthritis or hip dysplasia has been shown to negatively affect many daily life aspects. One aspect, however, which persists underestimated is sexual health. The number of total hip arthroplasties (THA) are increasing, especially in young patients who have high functional expectations, not only to pain relief, but to an increase in hip mobility and quality of life as well as sexual activity.Aim(1) to report the demographic factors, (2) the sexual activity before and after THA, as well as the concerns related to sexual activity after THA and (3) the patient-reported outcome measurements (PROMs) in sexually active male patients.MethodsWe evaluated the results of patients between 18 and 65 years of age following primary cementless short femoral stem THA using a direct anterior approach (DAA) at a midterm follow-up of 4 years. A web-based questionnaire (via SurveyMonkeyâ) was chosen to assess frequency, positions, complaints, fears, dealing with the questions and PROMs. Our patients sexual activity was via The Sexual Health Inventory for Men (SHIM) validated.ResultsPatients resumed their sexual activities after 6 weeks. The two main causes of difficulty in sexual activity before surgery were pain and limitation of the range of motion of the hip joint. Patients experienced less pain and an improvement in hip range of motion after THA. 89% of patients expressed a desire for more detailed and specific information on the subject. After 4 years follow-up our patients presented a significant improvement of the modified Harris Hip Score (mHHS) from 34.1 preoperative to 92.6 after THA.Strengths and LimitationsThe use of a web-based questionnaire showed a response quote of 78.3%, which is one of the highest (30 – 80%) in relation to its peers. First the option of closed answers is a limitation because patients may interpret the questions differently. Second, we included only patients who underwent uncemented short femoral stem THA using a DAA. ConclusionTHA improves the quality in sexual life, in relation to less pain and improvement in the range of motion, but not in the frequency of sexual activity.
Openvar: Functional Annotation Of Variants In Non-Canonical Open Reading Frames
Authors: Brunet Marie A , Leblanc Sébastien , Roucou Xavier 
Published: March 2022

Abstract
Background: Recent technological advances have revealed thousands of functional open reading frames (ORF) that have eluded reference genome annotations. These overlooked ORFs are found throughout the genome, in any reading frame of transcripts, mature or non-coding, and can overlap annotated ORFs in a different reading frame. The exploration of these novel ORFs in genomic datasets and of their role in genetic traits is hindered by a lack of software. Results: Here, we present OpenVar, a genomic variant annotator that mends that gap and fosters meaningful discoveries. To illustrate the potential of OpenVar, we analysed all variants within SynMicDB, a database of cancer-associated synonymous mutations. By including non-canonical ORFs in the analysis, OpenVar yields a 33.6-fold, 13.8-fold and 8.3-fold increase in high impact variants over Annovar, SnpEff and VEP respectively. We highlighted an overlapping non-canonical ORF in the HEY2 gene where variants significantly clustered.Conclusions: OpenVar integrates non-canonical ORFs in the analysis of genomic variants, unveiling new research avenues to better understand the genotype-phenotype relationships.
Research on the mechanism of emotional design in Chinese cultural and creative products
Authors: Feng Xin , Yu Lei , Wang Jingya , Kong Weixin , Chen Weiqing 
Published: March 2022

Abstract
The empowerment of design for cultural and creative products in the digital media era is further amplified by social media platforms,its emotional and subconscious impact on users is important and profound. In addition, the integration of cultural creativity into emotional design will become an important way to enhance regional and national cultural confidence. It is easy to see that product design with unique emotional design features will become an important driver of market development, but the research on the mechanism of the role of emotional design in cultural and creative products is still not clear and explicit.the paper compares four winning entries in the Cultural and Creative Product Design Competition and four commercial products in online stores through 176 valid questionnaires. The paper uses structural equations to analyze whether these criteria are accepted by consumers and the market, and whether there is a perception gap between the two .
Bronchial Thermoplasty Attenuates Cough Reflex Sensitivity in Severe Asthma: A Single-Center Retrospective Study with 2-year Follow-up
Authors: Hara Johsuke , Yamamura Kenta , Sakai Tamami , Kobayashi Takafumi , Ohkura Noriyuki , Watanabe Satoshi , Tambo Yuichi , Sone Takashi , Kimura Hideharu , Abo Miki , Kasahara Kazuo , Yano Seiji 
Published: March 2022

Abstract
Despite the relatively short follow-up period in our previous study, we had reported that increased cough reflex sensitivity (CRS) may predict the efficacy of bronchial thermoplasty (BT) for treating asthma. Herein, we examined whether CRS predicts the efficacy of BT 2 years after the final BT treatment. We also investigated the influence of BT on CRS. We reviewed 12 patients 2 years after their final BT treatment. CRS, asthma-related symptoms, asthma exacerbations, and cough-related quality of life were assessed at baseline and 2 years after BT. Six patients responded positively to BT (BT responders) and their asthma control improved. No significant difference in CRS at baseline was detected between the BT responders and nonresponders. In contrast, BT responders exhibited significant improvements in CRS 2 years after BT. CRS at baseline could not predict the BT efficacy after 2 years. This is the first report demonstrating BT desensitized CRS in consecutive case series.
Non-Surgical Management of the Gingival Smile with Botulinum Toxin A. A Systematic Review and Meta-Analysis.
Authors: Rojo-Sanchis Carolina , Montiel-Company José María , Tarazona-Álvarez Beatriz , Peiró-Guijarro María Aurora , Haas-Junior Orion Luiz , Guijarro-Martínez Raquel 
Published: March 2022

Abstract
Due to its aesthetic implications, gummy smile is a common reason for consultation. When it is caused by a vertical excess of the maxilla, the ideal solution is orthognathic surgery. However, in borderline cases and when the cause is hyperactivity of the upper lip levator muscle complex, alternative conservative solutions can be considered, such as the application of botulinum toxin A (BTX-A). The main objective of this systematic review and meta-analysis is to analyze the effectiveness of BTX-A in the treatment of gummy smile in short and medium term, to study its stability and to evaluate potential complications. A thorough search of the PubMed, Scopus, Embase, Web of Science and Cochrane databases and a grey literature search were conducted. The inclusion criteria were: studies with a sample size greater than or equal to 10 patients with gingival exposure greater than 2 millimeters in smile, treated with BTX-A infiltration. The benefit of BTX-A in terms of improvement of gummy smile is demonstrated, a statistically significant reduction in gummy smile is estimated by BTX-A therapy two weeks after its application. Its results gradually decrease over time, however they remain satisfactorily without returning to their initial values ​​after 12 weeks.
Person Identification by Evaluating Gait using 2D LiDAR and Deep Neural Network
Authors: Hasan Mahmudul , Hanawa Junichi , Goto Riku , Fukuda Hisato , Kuno Yoshinori , Kobayashi Yoshinori 
Published: March 2022

Abstract
Video-based recognition techniques are solemnly effective, and it comes to a new era of research nowadays. Yet again, it suffers some bottlenecks indeed. Situations, surroundings, and momentums may be disgraceful with all new inventions. So, to solve the drawbacks of technology is to imply a new technology on it. Biometric features are very authentic and high valued measures for human identifications. Most of the techniques are dependent on close contact with the subject. A gait is a pattern that performs by walking from the individual. Almost all studies of gait-based person identifications are performed by RGB or RGB-D cameras. Very few studies were done by using LiDAR data. Applying 2D LiDAR images for individual tracking and identification is superb when video surveillances fail to perform accurately due to environmental and imposed difficulties (i.e., disaster, rain, fog, smoke, snow, occlusion, cost, etc.). This research performed a comprehensive exhibition of 2D LiDAR data with a rigorous self-made dataset and customized residual neural network. We considered different experimental setups and found exciting precisions there. Our system is appropriate for recognizing a person based on his ankle level 2D LiDAR data.
Ataluren Prevented Bone Loss Induced by Ovariectomy and Aging in Mice Through the BMP-SMAD Signaling Pathway
Authors: Zeng Lijun , Gu Ranli , Shao Yuzi , Zhu Yuan , Xie Zhengwei , Liu Hao , Zhou Yongsheng 
Published: March 2022

Abstract
BackgroundBoth estrogen deficiency and aging may lead to osteoporosis. Developing novel drugs for treating osteoporosis is a popular research direction. Ataluren (ATA) is a nonaminoglycoside nonsense mutation inhibitor currently used clinically to treat muscular atrophy and Duchenne muscular dystrophy. However, little attention has been given to the role of Ataluren in osteogenesis, and it is unclear whether Ataluren can improve osteogenic differentiation of human- derived bone marrow mesenchymal stem cells (hBMMSCs) and treat osteoporosis.MethodsIn this study, we screened several potential therapeutic agents through a new deep learning-based efficacy prediction system (DLEPS) using transcriptional profiles for osteoporosis. We used ovariectomized (OVX) and aged female C57BL6 mice as bone loss models to demonstrate the role of preventing bone loss for Ataluren. The osteogenic differentiation of hBMMSCs was verified via qRT–PCR, staining and quantification of alkaline phosphatase and alizarin red S, and western blotting (WB), with and without an inhibitor of the BMP-SMAD signaling pathway and siBMP2. ResultsDLEPS screening led to a potential novel drug examinee, ataluren, for treating osteoporosis. Ataluren significantly reversed bone loss in ovariectomized mice. Next, ataluren significantly increased human bone marrow-derived mesenchymal stem cell (hBMMSC) osteogenic differentiation without cytotoxicity, indicated by the high expression index of osteogenic differentiation genes (OCN, BGLAP, ALP, COL1A, BMP2, RUNX2). Mechanistically, ataluren exerted its function through the BMP–SMAD pathway. Furthermore, it activated SMAD phosphorylation but osteogenic differentiation was attenuated by BMP2–SMAD inhibitors or small interfering RNA of BMP2. Finally, ataluren significantly reversed bone loss in aged mice. ConclusionsIn summary, our findings suggest that the DLEPS-screened ataluren may be a therapeutic agent against osteoporosis by aiding hBMMSC osteogenic differentiation.
Assessing The Low-Carbon City Pilot Policy On Carbon Emission From Consumption And Production In China: How Underlying Mechanism And Spatial Spillover Effect?
Authors: Ren Huimin , Gu Guofeng , Zhou Honghao 
Published: March 2022

Abstract
With the acceleration of industrialization and urbanization, China’s greenhouse gas emissions have gradually become one of the major constraints to its sustainable development. As a comprehensive environmental regulation policy, Low-carbon city pilot (LCCP) policy is widely used to mitigate carbon dioxide emissions and guide low-carbon development in China. Based on the panel data of 285 cities from 2003 to 2019 in China, this study mainly applied the staggered DID method to examine the effects of LCCP policy on carbon intensity (CI) and carbon emission per capita (CP) from production and consumption perspectives. The textual quantification and spatial DID method were also employed to explore the mechanism, heterogeneity and spatial spillover effect in an integrated manner. Our results show that: (1) The LCCP policy effectively reduced CI and CP, but these effects did not appear until the third year of implementation. The above conclusions passed a series of robustness and endogeneity tests. (2) Reducing industrial emission, improving technological innovation and optimizing the efficiency of energy usage were three important mechanisms to reduce CI and CP, validating the effectiveness of the LCCP policy. (3) Command-mandatory and voluntary LCCP policy tools achieved better results and the LCCP policy exerted a significant emission reduction effect on second-tier pilot cities as compared to others. (4) This abatement effect of LCCP policy had also demonstrated a spatial spillover impact on neighboring cities. Findings from this study provide empirical evidence for the promotion of low-carbon city pilots and offer a replicable and effective path to implement environmental regulation.
Clinical Analysis of Bronchoscope Diagnosis and Treatment for Airway Foreign Body Removal in Pediatric Patients
Authors: Han Lin-lin , Zhang Zhong-xiao , Tang Xiao-di , Ma Jing , Meng Chen , Li Chang-xiao 
Published: March 2022

Abstract
Background: With the wide application and technical development of flexible and rigid bronchoscopy, the method of removing airway foreign body has developed from the single use of foreign body forceps to different techniques for different foreign bodies.Methods:Selected 633 children who were diagnosed as airway foreign bodies by the Department of Respiratory Intervention, Children's Hospital affiliated to Shandong University from January 1, 2018 to December 31, 2021, and the airway foreign bodies were diagnosedvia bronchoscopy. After comprehensive evaluation about the nature of the foreign body in the airway, removed the foreign body with the foreign body forceps, freezing, laser, electrocoagulation, balloon and other techniques, observed the success rate of the foreign body removed from the airway, the percentage of the foreign body removed using different techniques, the operation time, and the incidence of post-adverse reactions during operation.Results:The success rate of using flexible bronchoscope alone to remove foreign bodies in the airway was 99.2%. After flexible bronchoscopy, 19 cases of foreign bodies were removed by vacuum suction alone, 513 cases were removed by foreign body forceps alone, 62 cases were combined with cryotherapy, 2 cases were electrocoagulation, 6 cases weremesh baskets, 3 cases were balloons, 5 cases were laser, and various 18 cases of foreign bodies were taken out by technical combination. 5 cases of flexible bronchoscope combined with rigid bronchoscope combined to remove foreign bodies. The operating time ranges from 5min-1h, with an average of 20minutes.There were 17 cases of hypoxemia (2.7%) during the operation, 36 cases (5.7%) of bleeding caused by airway mucosa injury after the treatment, and 70 cases (11.2%) of laryngeal edema. The total incidence of adverse reactions was 19.6%, there were no deaths due to foreign bodies and treatment.Conclusions According to the different properties of airway foreign bodies, it is safe and effective to select appropriate techniques to extract foreign bodies through the flexible bronchoscope, which can increase the removal rate of airway foreign bodies and reduce the occurrence of serious complications.
Cervical Bleeding with Cervical Stabilization During IUD Placement: Allis Clamp Versus Single-Tooth Tenaculum
Authors: Andrews Brooke , Quick Kristen , MacLeod Erin , Edwards Kayce , Rone Bryan 
Published: March 2022

Abstract
OBJECTIVETo evaluate whether the use of an atraumatic Allis clamp will result in less bleeding than the standard single-tooth tenaculum for cervical stabilization during intrauterine device (IUD) placement.METHODSA single blinded randomized controlled trial was conducted during insertions of IUDs between March 2017 and March 2018. University of Kentucky Institutional Review Board (IRB 16-1110-P3K) approval was obtained. Physicians were randomized to use either an Allis clamp or a single-tooth tenaculum for cervical stabilization. A post-procedure questionnaire was used to collect outcome measures as well as demographic and obstetric related factors.RESULTSOf the ninety-five participants, there was no difference in age, self-identified race/ethnicity, or the evaluated obstetric factors between the groups. Bleeding was present after clamp removal in 3 (6.3%) insertions using an Allis clamp and 26 (55.3%) insertions using a single-tooth tenaculum (RR = 0.113, CI=[0.037, 0.3481], p < 0.0001). There was no difference in IUD insertion success rates between the two clamps. There was no difference in the number of interventions required for hemostasis when bleeding occurred after clamp removal. Pain scores did not differ based on clamp type or age of patient, but were significantly different based on parity (p < 0.001) and IUD type (p < 0.003).CONCLUSIONThe Allis clamp should be used instead of a single-tooth tenaculum for cervical stabilization because of decreased incidence of clamp site bleeding with unchanged insertion success.The trial was retrospectively registered on 1/11/22 (trial registration number NCT05187078).
Intelligent Security Gate
Authors: Elwan Mohammed , Salem Amira , Fawky Hossam , Mahmoud Ahmed 
Published: March 2022

Abstract
Modern organizations have to use digital technology and digital transformation is the way to achieve this. Track employee attendance digitally and do not rely on outdated bookkeeping methods to improve the time spent during working hours, calculate annual leave for employees, and efficiently process payroll. In this research paper, a gate has been proposed through which employees' departure and attendance are calculated. And prevent any unwanted person from entering and at the same time, a unit can be added through which individuals are sterilized to the Prevention of the coronavirus. The proposed system showed high efficiency in recording the attendance and the departure of employees, as well as preventing any unwanted person from entering. The raspberry pi 4 model/ 8GB is used and the practical tests were conducted and applied to the system of employees of the Egyptian Academy of Engineering and Advanced Technology