bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid visual detection of Streptococcus suis and Actinobacillus pleuropneumoniae using duplex recombinase polymerase amplification combined with lateral flow dipsticks
Authors: Zhang Shanshan , Xie Hongbing , Liu Mingcheng , Zheng Airong , Yan Haixing , Duan Mingyuan , Wei Xiaobing , Hu Jianhe , Zhang Huihui , Xia Xiaojing 
Published: March 2022

Abstract
We established a rapid detection method based on recombinant enzyme polymerase amplification (RPA) for the simultaneous detection and identification of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) and Streptococcus suis (S. suis). A pair of primers and corresponding probes were designed for the glutamate dehydrogenase (gdh) gene of S. suis and the ApxIV gene of A. pleuropneumoniae to establish a dual RPA-LFD detection method for the rapid identification of S. suis and A. pleuropneumoniae. The specificity test showed that the amplification results for Pasteurella, E. coli, Salmonella, S. aureus, H. parasuis and A. hydrophila were all negative, indicating the good specificity of the method. The sensitivity test showed that the lowest nucleic acid concentration that was detectable with this method was 10− 5 ng/µL, which was significantly higher than that of PCR and RPA-Basic. S. suis and A. pleuropneumoniae reference strains, clinical isolates and 45 clinical lung tissue samples were detected with this method. The results showed that this method detected all reference strains and clinical isolates, which was consistent with the PCR detection results. Among the 45 clinical samples, 19 cases of S. suis and 1 case of A. pleuropneumoniae were detected, and there were no mixed infections. The detection rate was higher than that of bacterial isolation and the conventional PCR method, indicating that this method is very practical and suitable for the rapid clinical detection of S. suis and A. pleuropneumoniae. Compared with the traditional method, the dual RPA-LFD method has several advantages including a high specificity, high sensitivity, fast speed (detection time of 20 min) and minimal requirement for instruments and equipment; in addition, the method can realize the synchronous and rapid detection of S. suis and A. pleuropneumoniae and is helpful for the preliminary screening of clinical diseases.
Benefits of Global Earth Observation Missions for Exposure Estimation and Earthquake Loss Modelling – Evidence from Santiago de Chile, Chile
Authors: Geiß Christian , Priesmeier Peter , Pelizari Patrick Aravena , Calderon Angélica Rocio Soto , Schoepfer Elisabeth , Riedlinger Torsten , Vega Mabé Villar , María Hernán Santa , Zapata Juan Camilo Gómez , Pittore Massimiliano , So Emily , Fekete Alexander , Taubenböck Hannes 
Published: March 2022

Abstract
Exposure is an essential component of risk models and describes elements that are endangered by a hazard and susceptible to damage. The associated vulnerability characterizes the likelihood of experiencing damage (which can translate into losses) at a certain level of hazard intensity. Frequently, the compilation of exposure information is the costliest component (in terms of time and labor) of risk assessment procedures. Existing models often describe exposure in an aggregated manner, e.g., by relying on statistical/census data for given administrative entities. Nowadays, earth observation techniques allow the collection of spatially continuous information for large geographic areas while enabling a high geometric and temporal resolution. Consequently, we exploit measurements from the earth observation missions TanDEM-X and Sentinel-2, which collect data on a global scale, to characterize the built environment in terms of constituting morphologic properties, namely built-up density and height. Subsequently, we use this information to constrain existing exposure data in a spatial disaggregation approach. Thereby, we establish dasymetric methods for disaggregation. Results are presented for the city of Santiago de Chile, which is prone to natural hazards such as earthquakes. We present earthquake loss estimations and corresponding sensitivity concerning the resolution properties of the exposure data used in the model. Experimental results show how loss estimations vary substantially from a spatial perspective. As such, this study underlines the benefits of deploying modern earth observation technologies for exposure mapping and related earthquake loss estimation with enhanced accuracy properties.
A case of pulmonary artery intimal sarcoma diagnosed early using surgical biopsy
Authors: Fukuda Shota , Nishino Masaya , Sato Katsuaki , Tomizawa Kenji 
Published: March 2022

Abstract
Background: Pulmonary artery intimal sarcoma (PAIS) is extremely rare and is very difficult to differentiate from pulmonary embolism (PE). Herein, we describe a case in which PAIS was strongly suspected based on clinical findings, and early definitive diagnosis was obtained.Case presentation: A 70-year-old man was referred to our hospital for the investigation of multiple round shadows in the lung fields and an enlarged right pulmonary hilar vasculature on chest radiography. Chest enhanced-computed tomography showed multiple pulmonary nodules, and bilateral non-contiguous shadows in the main pulmonary arteries, extending from the main pulmonary arteries to the periphery on the right side. Pulmonary embolism was clinically excluded because there were no subjective symptoms and the D-dimer level was normal. Positron emission tomography showed abnormal accumulation in the intrapulmonary arterial shadows, pulmonary nodules, and left iliac bone. The patient was clinically diagnosed with a suspected PAIS with multiple pulmonary and bone metastases within 3 days of presentation at our hospital. We performed a thoracoscopic partial resection of the right upper lobe and obtained a histopathological definitive diagnosis of PAIS. As the positive result for MDM2, we attempted to participate in the investigator-initiated clinical trial of anti-MDM2 antibody (DS-3032b) in patients with MDM2 amplified intimal sarcoma (JMA-IIA00402). However, he was not able to participate in the clinical trial due to the lack of MDM2 gene amplification by fluorescence in situ hybridization. He received drug therapy with cytotoxic anticancer agents, a multitargeted tyrosine kinase inhibitor, or an immune checkpoint inhibitor but it was ineffective, and he died nine months after definitive diagnosis.Conclusions: In actual clinical practice, it is important to make early clinical and histological diagnosis, always keeping in mind the possibility of rare lung malignancies.
Identification of Patient Support Groups in Malaysia: An Exploratory Study
Authors: Saw Le Er , Ho Jacqueline , Tan May Loong 
Published: March 2022

Abstract
BackgroundPatient support groups (PSGs) are generally defined as a group of people with common experiences and concerns, and they provide emotional and moral support for one another. Despite their widely reported benefits, there are no studies conducted on the regulation of PSGs in Malaysia and their quality of support. We conducted this exploratory research to identify, map and describe PSGs in Malaysia aimed at identifying PSGs that might be interested in receiving training in understanding and using high-level research evidence such as Cochrane evidence. The ultimate goal is to empower patients to make evidence-based healthcare decisions.MethodsWe performed an online search and contacted practising physicians from various specialties for suggestions of known PSGs. We examined the websites of all identified PSGs and extracted data describing their organisation, purpose and function. Results A total of 44 PSGs meeting our criteria were included. Most of the PSGs identified catered to disease groups, mainly NGOs and informal Facebook groups. Most of them provided support to the patient and their family or caretakers in very similar ways, with some minor variations. About one-third had a stated mission and vision or objectives. None of the PSGs provided a description of their funding policy or provided any statement of awareness of conflict of interest (COI). Where information on funding sources was included it was not clear whether there could be a COI in it. Although the websites did not include advertising, one included a logo of a major pharma company. Only 4 included in any website scientific references to support the information included in the websiteConclusionsPSGs operating in Malaysia have a clear mission statement, vision and objectives on their website describing their intention and role in supporting patients in a holistic manner. They practise a partnership model with their users. Aspects that could be addressed include PSGs’ stance on COI and transparency in sources of funding. There may be a need to engage with PSGs to help them seek feedback from users and to help them understand and use reliable research in their education of their target patient groups.
1H, 13C, and 15N resonance assignments of human glutathione peroxidase 4
Authors: Furuita Kyoko , Inomata Kouki , Sugiki Toshihiko , Kobayashi Naohiro , Fujiwara Toshimich , Kojima Chojiro 
Published: March 2022

Abstract
Glutathione peroxidase 4 (GPx4) behaves as an antioxidant enzyme capable of directly reducing peroxidized phospholipids within cell membranes. Recently, GPx4 has attracted attention as a target molecule for cancer therapy because it induces the immortalization of cancer cells suppressing ferroptosis. In this study, to analyze the function and structure of GPx4 by solution NMR, we performed resonance assignments of GPx4 and assigned almost all backbone 1H, 13C, and 15N resonances and most of the side chain 1H and 13C resonances. Using these assignments, the secondary structure of GPx4 was analyzed by the TALOS + program. GPx4 has six helices and seven strands. Then, the backbone dynamics were examined by the {1H}-15N heteronuclear NOE experiment. GPx4 was found to be rigid except for a short loop region. These results will provide bases for functional analysis and the first solution structure determination of GPx4.
Multi-criteria group decision-making with cloud model and TOPSIS for alternative selection under uncertainty
Authors: Zhang Limao , Chen Weiyi 
Published: March 2022

Abstract
A novel hybrid soft computing approach that integrates the cloud model and the Technique of Order Preference Similarity to the Ideal Solution (TOPSIS) is developed to deal with multi-criteria decision making (MCDM) problems under uncertainty. Cloud models are used to derive linguistic concepts in numerical measures with the consideration of uncertainty. TOPSIS is used to determine the ranking of various alternatives with the evaluation matrices processed by the cloud model. The Monte Carlos simulation is implemented to model the uncertainty underlying the characterization of input factors and obtain their global sensitivities. An evaluation system with 16 criteria is formulated to discover the optimal alternative for tunnel excavation. A realistic tunnel section case in China is selected to demonstrate the adaptability and significance of the proposed approach. Results imply that (1) Among the 5 candidate tunneling methods, the shield tunnel boring machine (TBM) is determined as the optimal method to excavate the tunnel section in the case; (2) The inconsistency in results occur during the simulation in the presence of uncertainty, where there is 13% probability that soft-rock TBM will rank first in the case study; (3) With the increase of Entropy in cloud models, the degree of inconsistency will grow significantly. The developed approach can be used as a decision tool to provide insights into a better understanding of multi-source information fusion for alternative selection uncertainty with variations in the simulation and sensitivity analysis.
Travel related histoplasmosis – a diagnostic challenge in a patient with tumor necrosis factor alpha (TNF-α) inhibitor therapy
Authors: Hofmann Eveline , Mühlethaler Konrad , Pollak Matthias , Ott Daniel , Bienz Nora , Zimmerli Stefan , Hirzel Cédric 
Published: March 2022

Abstract
Introduction: In a non-endemic setting, disseminated histoplasmosis is a rare travel-related health problem of immunosuppressed returnees from endemic regions.Methods: We describe the case of a 68-year-old man with rheumatoid arthritis and tumor necrosis factor alpha (TNF-α) inhibitor treatment-related immunodeficiency, who suffered from disseminated histoplasmosis after traveling to Brazil. Based on this case, we discuss challenges and pitfalls associated with the diagnosis of disseminated histoplasmosis in a non-endemic setting.Results: The disease mimicked a hemophagocytic lymphohistiocytosis (HLH) like syndrome. Histoplasma capsulatum was microscopically detected in bronchoalveolar fluid and bone marrow aspirate smears, but was initially misclassified as Leishmania spp., another class of pathogens, which may cause HLH like syndromes in immunocompromised individuals. Discussion: Since the clinical symptoms of histoplasmosis are nonspecific and physicians in non-endemic regions might not be familiar with this disease pattern, there is a risk of delayed diagnosis of travel related cases. Taking a thorough travel history is key in unclear cases of illness in immunocompromised patients.
hsa-miR-181-5p inhibits human immunodeficiency virus type 1 replication by downregulating DDX3X expression
Authors: han dating , lu xiangyun , wu nanping 
Published: March 2022

Abstract
The adverse effects of antiviral therapy have prompted research into novel treatment strategies. miR-181 has been shown to be negatively correlated with the HIV viral load. We predicted that miR-181 could target DDX3X, a host factor involved in HIV nuclear export leading to the inhibiting of HIV replication. To verify our hypothesis, an miR-181 mimic was transfected into Jurkat cells infected with pNL4-3 wild strain and HIV replication-competent H9-IIIB cells. Besides the reporter gene plasmid containing the DDX3x mRNA sequence was transfected into 293T cells. We found that miR-181 significantly reduced HIV viral protein Gag(p24) Tat and Rev and downregulated the expression of mRNAs and protein targeting DDX3X. Our results confirmed that miR-181 was indeed involved in regulating the level of HIV viral replication in lymphocytes by downregulating the level of DDX3X expression. Our research results provide a research basis for the future development of new antiviral drugs for HIV.
Identification of RP11-770J1.4-CTXN1 as an immunomodulatory axis in Low-Grade Glioma
Authors: Zhuang Qiyuan , Liu Chaxian , Hu Yihan , Liu Ying , Lyu Yingying , Liao Yuhen , Chen Liang , Yang Hui , Mao Ying 
Published: March 2022

Abstract
Human gliomas are lethal brain cancers whose progression is complexly regulated. Emerging evidence reveals that long non-coding RNAs (lncRNAs), functioning as competing endogenous RNAs, participate in regulatory networks in tumors. Here, we comprehensively integrated and analyzed expression profiles for RNAs in low-grade glioma (LGG). Functional annotation analysis suggested that immune-related signature is involved in progression of glioma. Importantly, survival analysis showed that features in networks exhibited close relations to the prognosis. Further experiments showed that RP11-770J1.4 regulated the expression of CTXN1 by sponging hsa-miR-124-3p. Correlation analysis and GSEA enrichment revealed that RP11-770J1.4 is an immune-related lncRNA. Single cell RNA sequencing (scRNA-seq) combined with glioma stem cells (GSCs) model and patient samples revealed CTXN1 distributed mainly in tumor core, rather than periphery. Utilizing immunohistochemistry staining in low-grade glioma cases, CTXN1 negatively correlated to CD8+ T cells infiltration. Knocking down Ctxn1 in GL261 cell decreased tumor weight and prolong survival time in vivo, while increased tumor CD8+ T cells infiltration. These findings revealed RP11-770J1.4-CTXN1 as novel immune regulator axis in glioma and provided potential therapeutic targets for LGG treatment.
Vitamin D status in Psoriasis: Impact and Clinical Correlations
Authors: Bhat Ghulam Hassan , Guldin Sadaf , Khan Mosin Saleem , Yasir Mir , Prasad Ganesh 
Published: March 2022

Abstract
Psoriasis is a continuing, periodic, immune‑mediated, fiery skin disease branded by hyper proliferation of epidermal keratinocytes and accompanying with inflammatory cellular infiltrate in both dermis and epidermis. Immunomodulation could be an important effect of vitamin D in Psoriasis. This case-control study was designed to measure serum 25-hydroxy vitamin D levels in patients with psoriasis and healthy controls and to find out clinical correlation, if any. Two hundred forty (n=240) subjects (120 cases and 120 controls) were taken for the study. Cases and controls were frequency matched with respect to age, gender and smoking status. Various demographic and clinical details were taken using a questionnaire. Chemiluminescence Micro Particle Immunoassay was used to estimate serum 25-hydroxy vitamin D levels. The vitamin D deficiency in psoriasis patients was 60.0% vs. 17.5% in controls (P<0.001) with mean vitamin D levels of 28.3 ± 13.9 ng/ml in psoriasis patient’s vs 37.9 ± 9.7 ng/ml in controls. Vitamin D deficiency was found to be associated with psoriasis independently of gender, age, smoking status, family history, hypertension, chronic medication, nail changes, duration of symptoms and severity of disease. Vitamin D levels were seven times lower in patients with Psoriasis as compared to controls. Reduced vitamin D levels are related to duration and clinical severity of the disease. Early detection of vitamin D deficiency and timely intervention could lead to better clinical outcome and improved quality of life in psoriasis patients.
Tissue Characteristics of Residual Lesion in Patients With Acute Coronary Syndrome Caused by Plaque Rupture Versus Plaque Erosion: A Single-center, Retrospective, Observational Study
Authors: Ando Hirohiko , Suzuki Wataru , Ohashi Hirofumi , Nakano Yusuke , Takashima Hiroaki , Fujimoto Masanobu , Sawada Hiroaki , Goto Reiji , Suzuki Akihiro , Sakurai Shinichiro , Amano Tetsuya 
Published: March 2022

Abstract
PurposePatients with acute coronary syndrome (ACS), frequently caused by plaque rupture (PR), often have vulnerable plaques in residual and culprit lesions. However, whether this occurs in patients with plaque erosion (PE) as well is unknown. Therefore, we compared the tissue characteristics of residual lesions in patients with PE and those with PR.MethodsThe data of 88 patients with ACS who underwent both optimal coherence tomography (OCT) and intravascular ultrasound (IVUS) were retrospectively analyzed. Based on plaque morphology of the culprit lesions identified using OCT, patients were classified into PE and PR groups. The tissue characteristics of residual lesions evaluated using integrated backscatter IVUS were compared between the two groups after percutaneous coronary intervention. ResultsPatients were classified into the PE group (n=23) and PR group (n=35). The PE group had a significantly lower percent lipid volume and a higher percent fibrous volume than those of the PR group (35.0±17.8% vs. 49.2±13.4%, p<0.001; 63.2±17.1% vs. 50.3±13.1%, p=0.002, respectively). Receiver operating characteristic curve analysis revealed that the percent lipid volume in the residual lesions was a significant discriminant factor in estimating the plaque morphology of the culprit lesion (optimal cut-off value, <43.5%; sensitivity and specificity values were 73.9% and 68.6%, respectively).ConclusionsPatients with PE had a significantly lower percent lipid volume and a significantly higher percent fibrous volume in the residual lesions than in those with PR, suggesting that the nature of coronary plaques in patients with PE is different from that in patients with PR.
Individual experience influences reconstruction of division of labour under colony disturbance in a queenless ant species (Diacamma cf. indicum)
Authors: Tanaka Yasunari , Hojo Masaru K. , Shimoji Hiroyuki 
Published: March 2022

Abstract
BackgroundDivision of labour (DOL) is ubiquitous across biological hierarchies. In eusocial insects, DOL is often characterized by age-related task allocation, but workers can flexibly change their tasks, allowing for DOL reconstruction in fluctuating environments. Behavioural change driven by individual experience is regarded as a key to understanding this task flexibility. However, experimental evidence for the influence of individual experience is remains sparse. Here we tested the effect of individual experience on task choice in the queenless ponerine ant (Diacamma cf. indicum) from Japan.ResultsWe confirmed that both nurses and foragers shifted to vacant tasks when the colony composition was biased to one or the other. We also found that task-shifted foragers continue nursing for a long time in disturbed colonies, whereas task-shifted nurses persist in their original task, even if they recently foraged. ConclusionsTaken together, our results suggest that individual experience decreases the response threshold of original foragers, as they continue to be specialist nurses in a disturbed colony. However, original nurses do not appear strongly affected by having forager experience and revert to being nurses. Therefore, while individual experience does have an effect, other factors, such as reproductive ability, are clearly required to understand DOL maintenance in fluctuating environments.
Treatment of osteoporotic vertebral compression fracture by precise injection and staged perfusion of bone cement kyphoplasty: a comparison study
Authors: Huang Jian , Huang Jun , Zhou Zongbo , Lu Zhifu , Fu Chuangong 
Published: March 2022

Abstract
Study design: Retrospective cohort study.Objective: To explore the clinical effect of precise injection and staged perfusion of bone cement kyphoplasty in the treatment of osteoporotic vertebral compression fracture.Methods: 110 patients treated with kyphoplasty from January 2020 to June 2021 were selected and divided into experimental group (n = 55) and control group (n = 55) according to different surgical methods. The experimental group was treated with precise injection and staged perfusion of bone cement kyphoplasty, while the control group was treated with traditional kyphoplasty. The operation time,intraoperative blood loss and amount of bone cement injection were recorded. The pain improvement was evaluated by VAS score. The operation effect were evaluated by anterior height of injured vertebral body, middle height of injured vertebral body, wedge angle of injured vertebral body and distribution grade of bone cement. The incidence of surgical complications was evaluated by the number of bone cement leakage.Results: There were no significant difference in the operation time, intraoperative blood loss and the amount of bone cement injection in two groups. There were no significant difference in VAS scores at 2h, 4h and 48h after operation between the two groups (P > 0.05); There were no significant difference in the ratio of anterior height of injured vertebral body between the two groups on the third day after operation and the last follow-up (P > 0.05); There were no significant difference in the ratio of middle height of injured vertebral body between the two groups on the third day after operation and the last follow-up (P > 0.05); There were no significant difference in wedge angle of injured vertebral body between the two groups at the third day after operation and the last follow-up (P > 0.05). There was significant difference in the distribution grade of bone cement between the two groups (P < 0.01); There was significant difference in the number of bone cement leakage between the two groups (P < 0.01), In cases of bone cement leakage, there were 1 case of type C, 1 case of type S in the experimental group, 7 cases of type C and 2 cases of type S in the control group.Conclusion: Precise injection and staged perfusion of bone cement kyphoplasty in the treatment of osteoporotic vertebral compression fracture can effectively reduce surgical complications and improve surgical efficacy.