bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bile Acid Conjugation on Solid Nanoparticles Enhances ASBT‐Mediated Endocytosis and Chylomicron Pathway but Weakens the Transcytosis by Inducing Transport Flow in a Cellular Negative Feedback Loop
Authors: Deng Feiyang , Kim Kyoung Sub , Moon Jiyoung , Bae You Han 
Published: June 2022

AbstractBile acid‐modified nanoparticles provide a convenient strategy to improve oral bioavailability of poorly permeable drugs by exploiting specific interactions with bile acid transporters. However, the underlying mechanisms are unknown, especially considering the different absorption sites of free bile acids (ileum) and digested fat molecules from bile acid‐emulsified fat droplets (duodenum). Here, glycocholic acid (GCA)‐conjugated polystyrene nanoparticles (GCPNs) are synthesized and their transport in Caco‐2 cell models is studied. GCA conjugation enhances the uptake by interactions with apical sodium‐dependent bile acid transporter (ASBT). A new pathway correlated with both ASBT and chylomicron pathways is identified. Meanwhile, the higher uptake of GCPNs does not lead to higher transcytosis to the same degree compared with unmodified nanoparticles (CPNs). The pharmacological and genomics study confirm that GCA conjugation changes the endocytosis mechanisms and downregulates the cellular response to the transport at gene levels, which works as a negative feedback loop and explains the higher cellular retention of GCPNs. These findings offer a solid foundation in the bile acid‐based nanomedicine design, with utilizing advantages of the ASBT‐mediated uptake, as well as inspiration to take comprehensive consideration of the cellular response with more developed technologies.
Unified Picture of (Non)Hysteretic Oxygen Capacity in O3‐Type Sodium 3d Layered Oxides
Authors: Lee Jaewoon , Park Sangeon , Choi Gwanghyeon , Kwon Dohyeong , Kim Jongbeom , Kim Hyungjun , Cho Maenghyo , Kim Duho 
Published: June 2022

AbstractA unified picture of the generalized mechanism that elucidates the (non)hysteretic oxygen capacities of O3‐type Na1−x[Li2/6Mn3/6M1/6]O2 (M = Mn4+, Ni4+, and Ti4+) layered oxides is suggested to provide a critical factor in inducing ideal reversibility for an oxygen redox (OR) reaction using the new concept, the “potential‐pillar” effect. Considering that there is no formation of interlayer oxygen–oxygen (OO) dimers at x = 1.0 in Na1−x[Li2/6Mn3/6Ti1/6]O2, the phase stabilities reveal that the biphasic reaction occurs in Na1−x[Li2/6Mn3/6Ni1/6]O2 (0.5 ≤ x ≤ 1.0), and the monophasic reaction takes place in Na1−x[Li2/6Mn3/6Ti1/6]O2 during desodiation. The electronic structures of cations and anions unambiguously show OR reactions over the full vacancy range, and the oxygen ions comprising TiO6 are relatively deactivated compared with those of NiO6 upon electrochemical OR reaction. This is deemed as an intriguing “potential‐pillar” effect, in which the chemically stiff Ti4+(3d)O(2p) bond locally retains a strong electrostatic repulsion between the mixed layers. This unified concept based on an in‐depth understanding of the three cathode models not only accounts for the origin of the (non)hysteretic oxygen capacities, but also provides an exciting local structure viewpoint for harnessing the full potential of OR reactions for high energy‐density sodium‐ion batteries.
Synthesis of self‐healing soybean oil‐based waterborne polyurethane based on Diels–Alder reaction
Authors: Chen Liang , Dai Zhuding , Lou Wenxue , Jiang Pingping , Zhang Pingbo , Bao Yanmin , Gao Xuewen , Xia Jialiang 
Published: June 2022

AbstractIn order to endow water‐based polyurethane with special functionality, vegetable oil‐based waterborne polyurethane with self‐healing properties is synthesized. Firstly, soybean oil‐based emulsifier (GA‐ESO) was prepared by epoxy soybean oil and glutaric acid without solvent and catalyst. Then, two Diels−Alder (DA) diols were prepared by the DA reaction between N,N′‐(4,4′‐methyldiphenyl) bismaleimide, 4‐maleimide phenol and furfuryl alcohol, respectively. Finally, GA‐ESO and DA diols used as the raw material was reacted with isophorone diisocyanate to synthesize a series of soybean oil‐based waterborne polyurethane with self‐healing behavior. The effects of DA diol on the properties of polyurethane films and the self‐healing process and mechanism of polyurethane membrane were investigated. The results showed that after the addition of DA diol, the particle size of polyurethane emulsion increased from 28.8 to 85.3 nm, the tensile strength was improved from 0.3 to 12.6 MPa, and the water contact angle was enhanced from 68.5° to 84.2°. In addition, it can be observed that with the addition of DAdiol, the polyurethane film had self‐healing behavior at high temperature, and the highest self‐healing efficiency can reach 100%.
QM/MM Calculations Suggest Concerted O−O Bond Cleavage and Substrate Oxidation by Nonheme Diiron Toluene/o‐Xylene Monooxygenase
Authors: Zhou Tai‐Ping , Deng Wen‐Hao , Wu Yuzhou , Liao Rong‐Zhen 
Published: June 2022

AbstractThe nonheme diiron toluene/o‐xylene monooxygenase (ToMO) is the most studied toluene monooxygenase that mediates an aromatic hydroxylation reaction. In this work, QM/MM calculations were performed to understand the reaction mechanism. It is revealed that the μ‐η2:η2 peroxodiferric species is the reactive intermediate after the binding of the O2 molecule to the reduced diferrous center. Subsequently, both a stepwise and a concerted mechanism involving the critical O−O bond cleavage and C−O bond formation were considered. The latter was calculated to be more favorable, suggesting that the formation of a high‐valent diferryl Q intermediate is not needed. The isomeric formation of the phenol product was found very facile. The first step was calculated to be rate‐limiting, with a barrier of 17.6 kcal/mol for the ortho‐hydroxylation.
Compartmentalized microfluidic device for in vitro co‐culture of retinal cells
Authors: Jahagirdar Devashree , Yadav Shital , Gore Manish , Korpale Vikram , Mathpati Channamallikarjuna S. , Chidambaram Subbulakshmi , Majumder Abhijit , Jain Ratnesh , Dandekar Prajakta 
Published: June 2022

AbstractThe investigation is focused on the development of a compartmentalized microfluidic device for coculturing the cells of crucial retinal cellular layers and assessing cell‐to‐cell interactions. A perfusion‐based microfluidic co‐culture device was employed and computationally validated for determining the pressure drop and fluid flow rate within the device microchannels. Fabrication was performed using PDMS polymer and coating of fibronectin and collagen facilitated adherence of the cells over the glass surface. Microfluidic device successfully supported cell proliferation, under continuous perfusion of 1 μl min‐1 flow rate. The barrier integrity of this coculture was confirmed by evaluating the permeability of fluorescently labeled molecules. The coculture expressed characteristic phenotypic protein markers like recoverin, PAX6, for retinal precursor cells, and RPE65 for retinal epithelial cells. The coculture also exhibited basal expression of TNF‐α under normal conditions. Differentiated photoreceptor cells positively expressed rhod inherently possess sensitivity toward violet/blue light, which was validated in R28 cells by exposure to light having a wavelength of 405 nm, which significantly decreased cell viability via increased TNF‐α production and reduced rhodopsin expression. This proof‐of‐concept investigation proved the functionality of the retinal coculture, which may be used as an appropriate perfusion‐based, preclinical tool for the evaluation of novel retinal drugs and delivery systems.
Enantioselective halogenation via asymmetric phase‐transfer catalysis
Authors: Lee Sunggi , Chung Won‐jin 
Published: June 2022

AbstractThe asymmetric phase‐transfer catalysis (PTC) is a flourishing field of contemporary synthetic organic chemistry, and this prominent methodology has been tremendously successful in enantioselective halogenations. Both electrophilic and nucleophilic reaction manifolds were enabled through the exploitation of highly ordered ion pairing and/or hydrogen‐bonding interactions around a carefully designed chiral phase‐transfer catalyst with an insoluble halogenating reagent as well as a suitable substrate. Fluorination has been the most fruitful, and encouraging results have also been documented with heavier halogens. This review surveys examples of various enantioselective halogenations via the asymmetric PTC from its beginning to prosperity over the past decade.
Modeling Parkinson's disease‐related symptoms in alpha‐synuclein overexpressing mice
Authors: Aniszewska Agata , Bergström Joakim , Ingelsson Martin , Ekmark‐Lewén Sara 
Published: June 2022

AbstractBackgroundIntracellular deposition of alpha‐synuclein (α‐syn) as Lewy bodies and Lewy neurites is a central event in the pathogenesis of Parkinson's disease (PD) and other α‐synucleinopathies. Transgenic mouse models overexpressing human α‐syn, are useful research tools in preclinical studies of pathogenetic mechanisms. Such mice develop α‐syn inclusions as well as neurodegeneration with a topographical distribution that varies depending on the choice of promoter and which form of α‐syn that is overexpressed. Moreover, they display motor symptoms and cognitive disturbances that to some extent resemble the human conditions.PurposeOne of the main motives for assessing behavior in these mouse models is to evaluate the potential of new treatment strategies, including their impact on motor and cognitive symptoms. However, due to a high within‐group variability with respect to such features, the behavioral studies need to be applied with caution. In this review, we discuss how to make appropriate choices in the experimental design and which tests that are most suitable for the evaluation of PD‐related symptoms in such studies.MethodsWe have evaluated published results on two selected transgenic mouse models overexpressing wild type (L61) and mutated (A30P) α‐syn in the context of their validity and utility for different types of behavioral studies.ConclusionsBy applying appropriate behavioral tests, α‐syn transgenic mouse models provide an appropriate experimental platform for studies of symptoms related to PD and other α‐synucleinopathies.
A novel pyroptosis risk model composed of NLRP6 effectively predicts the prognosis of hepatocellular carcinoma patients
Authors: Gao Xin , Wang Wen‐Xin , Zhang Xiao‐Lan 
Published: June 2022

AbstractBackgroundPyroptosis is a unique inflammatory‐related cell death process, and inflammation is considered to be a key factor in hepatocellular carcinoma (HCC). However, the pyroptosis landscape in HCC has not been thoroughly studied.MethodsClinical data and RNA sequencing data of HCC patients were collected from The Cancer Genome Atlas database, and differentially expressed genes (DEGs) associated with pyroptosis were discovered. The relationship between DEGs and prognosis was studied. Using The Cancer Genome Atlas cohort, a least absolute shrinkage and selection operator regression model was built on the basis of pyroptosis‐related DEGs, which was then verified by the Gene Expression Omnibus (GEO) cohort. Functional enrichment analysis and immunological states were also studied between distinct risk subgroups. Finally, the potential tumor suppressive function of NLRP6 in HCC was analyzed.ResultsIn total, 26 pyroptosis‐related DEGs were identified. Consensus clustering results showed patients with high levels of pyroptosis were associated with higher tumor stage, grade, and poor prognosis. The least absolute shrinkage and selection operator risk model was built using six genes linked with prognosis (GSDMC, GSDME, NOD2, NLRP6, CASP8, and SCAF11). Risk score was an independent risk factor that suggested shortened overall survival in both the training cohort (HR: 3.52, 95% CI: 1.351–9.193) and validation cohort (HR: 3.31, 95% CI: 1.435–7.617). Meanwhile, the low‐risk population had higher immunological activity. We also found a novel potential tumor suppressor gene NLRP6, which may negatively regulate the E2F and MYC pathways and be associated with higher immune cell infiltration levels, which lead to better prognosis.ConclusionsThis study revealed the pyroptosis landscape of HCC and provided a promising clinical prognosis evaluation model. Additionally, some new targets related to prognosis such as NLRP6 are proposed for further study.
Polymorphisms in lncRNA CCAT1 on the susceptibility of lung cancer in a Chinese northeast population: A case–control study
Authors: Ji Yangtao , Yang Yue , Yin Zhihua 
Published: June 2022

AbstractObjectTo explore the association of rs1948915, rs7013433 in long noncoding RNA (lncRNA) CCAT1 and rs6983267 in MYC enhancer region with the risk of lung cancer in a Chinese northeast population, a case–control study was conducted.MethodsThe hospital‐based case–control study contained 669 lung cancer patients and 697 healthy controls. Taqman® Probe allele resolution was used for genotyping. The differences between the case–control groups were analyzed using Student t‐test and chi‐square test. Logistic regression analysis was used to assess the relationship between the genotypes and the risk of lung cancer. Cross‐generation analysis was used to explore the relationship between gene–environment interaction and lung cancer.ResultsThere was no association between the three selected single‐nucleotide polymorphisms (SNPs) and the susceptibility of lung cancer. Rs1948915 CT was correlated with lung adenocarcinoma. In female stratification, rs1948915 CT/CC was associated with a decreased susceptibility of lung cancer significantly. Additionally, the additive and multiplicative interaction models showed that there was no interaction between the three selected SNPs and smoking status in lung cancer.ConclusionsThere may be an association between lung adenocarcinoma and rs1948915 polymorphism in the Chinese northeast population, while rs7013433 and rs6983267 might have no association. There was no interaction between the three selected SNPs and smoking status.
Toward a unified theory of technology continuance: Effects of conscious versus unconscious factors
Authors: Nguyen Dung Minh , Chiu Yen‐Ting Helena , Vu Huy Quang , Nguyen Trieu 
Published: June 2022

AbstractThe containment measures for the COVID‐19 pandemic, including social distancing, lockdown or quarantining, have led to a paradigm shift in people's life. In the wake of the pandemic, many people have begun adopting technology as a way to maintain their daily activities and limit face‐to‐face human interactions. While the pandemic has facilitated the widespread use of technology, little attention has been paid to how consumers' intention to continue using technology is affected by their rational evaluation and habitual response, simultaneously. To address this knowledge gap, this study employs the expectation‐confirmation model as a theoretical lens to propose the research model and explain the antecedents of technology continuance intention from both perspectives of conscious versus unconscious responses. The conscious response is reflected in perceived value and satisfaction, whereas the unconscious response is characterized as habit. The proposed research model and hypotheses are supported by the data collected from 308 online food delivery service users. Our results show that habit is driven by perceived value and satisfaction, and then mediates the effects of them on continuance intention. Moreover, habit exerts the strongest effect on continuance intention and weakens the importance of perceived value as well as satisfaction to continuance intention. This study is expected to set the theoretical foundation for more future research that focus on the exploration of the conscious versus unconscious nature of consumers' technology continuance. The study concludes with practical implications and suggestions for catering businesses and relevant stakeholders.
Procedural results and in‐hospital outcomes of percutaneous coronary intervention for chronic total occlusion in patients with reduced left ventricular ejection fraction: Sub‐analysis of the Japanese CTO‐PCI Expert Registry
Authors: Kobayashi Norihiro , Ito Yoshiaki , Kishi Koichi , Muramatsu Toshiya , Okada Hisayuki , Oikawa Yuji , Kawasaki Tomohiro , Yoshikawa Ryohei , Tanaka Hiroyuki , Katoh Osamu 
Published: June 2022

AbstractObjectivesTo evaluate the procedural results and in‐hospital outcomes of percutaneous coronary intervention (PCI) for chronic total occlusion (CTO) in patients with reduced left ventricular ejection fraction (LVEF).BackgroundWhile the technical success of general CTO‐PCI has improved, CTO‐PCI patients with reduced LVEF remain at high‐risk for adverse events.MethodsThe data of 820 patients with LVEF ≤ 35% (Group 1), 1816 patients with LVEF = 35%–50% (Group 2), and 5503 patients with LVEF ≥ 50% (Group 3), registered in the Japanese CTO‐PCI Expert Registry from January 2014 to December 2019, were retrospectively analyzed. The primary endpoint was in‐hospital major adverse cardiac or cerebrovascular events (MACCEs), including death, myocardial infarction, stent thrombosis, stroke, and emergent revascularization. Secondary endpoints included procedural details, guidewire success, and technical success.ResultsThere were no differences in guidewire and technical success rates between the groups. In‐hospital MACCEs was significantly higher in Group 1 (Group 1 vs. Group 2 vs. Group 3: 3.4% vs. 1.7% vs. 1.5%, p = 0.001) and was especially driven by death (1.3% vs. 0.3% vs. 0.1%, p < 0.001) and stroke (0.7% vs. 0.2% vs. 0.2%, p = 0.007). Multivariate analysis showed that LVEF ≤ 35% (odds ratio [OR]; 1.58, 95% confidence interval [CI]; 1.04–2.41, p = 0.03) and New York Heart Association (NYHA) class ≥ 3 (OR; 2.01, 95% CI; 1.03−3.93, p = 0.04) were predictors of in‐hospital MACCEs.ConclusionsIn‐hospital MACCEs were significantly higher in patients with LVEF ≤ 35%. LVEF ≤;35% and NYHA class ≥ 3 were predictors of in‐hospital MACCEs after CTO‐PCI.
Optimal electric load forecasting for systems by an adaptive Crow Search Algorithm: A case study
Authors: Li Bo , Sun Hongbin , Teimourian Milad 
Published: June 2022

SummaryIn this paper, a new technique is suggested for best electric load forecasting of South Korea. The proposed study is based on an improved metaheuristic methodology. This paper proposes a new improved version of Support Vector Machine for Regression (SVR) base on the proposed algorithm. The algorithm is based on a modified version of Crow Search Algorithm (ACSA), where, by combining by the SVR, generates an efficient classifier. Although, different drawbacks of using original CSA are stated, the proposed ACSA method provides a proper modification for resolving of these shortcomings. The forecasting model has been then performed to a historical data from South Korea to indicate the algorithm efficiency and the results are compared with some other methods, including SVR‐CGA, SVR‐CAS, ANN, and simple Regression to show the suggested method performance. Final results show that MAPD, MSE, and RMSE values of the test data with lower values for South, North, Center, and East, provided better results based on the proposed SVR‐ACSA based on different values of center and kernel of the SVR. Experimental results on the case study shows that the minimum MAPD has been added which is happened in Northern regional is 1.1062, 1.1336, 1.1065, 1.02, 2.23, 1.96, and 1.49 for SVR‐ACSA, SVR‐CGA, SVR‐CAS, ANN, Regression, LSTM‐RNN, and ELM which indicates the method's higher efficiency.
Cognitive emotion regulation strategies in relation to treatment outcome in a clinical sample of adolescents with personality disorders
Authors: Boon Albert Eduard , Hauber Kirsten 
Published: June 2022

AbstractThe aim of this study was to examine cognitive emotion regulation strategies (CERS) of help‐seeking adolescents diagnosed with personality disorders. At pre‐treatment, patients (N = 116) were found to use some maladaptive but also some adaptive CERS more often than adolescents from the general population. Less than 4% of these pre‐treatment CERS predicted treatment outcome. In patients whose treatment outcome according to the Symptom Checklist‐90 (SCL‐90) showed significant improvement (N = 75), a reduction of maladaptive CERS and an increase of adaptive CERS occurred. Patients that were unchanged or deteriorated (N = 41) showed no significant changes in CERS. In conclusion, pre‐treatment CERS are not predictive for treatment outcome in this sample of adolescents diagnosed with personality disorders. Even though patients who use more adaptive and less maladaptive CERS have fewer symptoms, the relationship between these CERS and symptoms in this group of severe patients remains unclear.
Combined Conventional Synthetic Disease Modifying Therapy vs. Infliximab for Rheumatoid Arthritis: Emulating a Randomized Trial in Observational Data
Authors: Barbulescu Andrei , Askling Johan , Saevarsdottir Saedis , Kim Seoyoung C. , Frisell Thomas 
Published: June 2022

Observational studies are often considered unreliable for evaluating relative treatment effectiveness, but it has been suggested that following target trial protocols could reduce bias. Using observational data from patients with rheumatoid arthritis (RA) in the Swedish Rheumatology Quality Register (SRQ), between 2006 and 2020, we emulated the protocol of the Swedish Farmacotherapy trial (SWEFOT) and compared the results. SWEFOT was a pragmatic trial nested in SRQ, between 2002 and 2005, where methotrexate (MTX) insufficient responders were randomized to receive additional infliximab or sulfasalazine (SSZ) + hydroxychloroquine (HCQ). Patients with RA initiating infliximab (N = 313) or SSZ + HCQ (N = 196) after MTX were identified in SRQ and the Prescribed Drugs Register, mimicking the SWEFOT eligibility criteria. The primary outcome was the proportion of European Alliance of Associations for Rheumatology (EULAR) good responders at 9 months, classifying patients who discontinued treatment as “nonresponders.” Through sensitivity analyses, we assessed the impact of relaxing eligibility criteria. The observed proportions reaching EULAR good response were close to those reported in SWEFOT: 39% (vs. 39% in SWEFOT) for infliximab and 28% (vs. 25%) for SSZ + HCQ. The crude observed response ratio was 1.39 (95% confidence interval (CI) 1.04–1.86), increasing to 1.48 (95% CI 0.98–2.24) after confounding adjustment, compared to 1.59 (95% CI 1.10–2.30) in SWEFOT. Results remained close to SWEFOT when relaxing eligibility criteria until allowing prior disease‐modifying anti‐rheumatic drug (DMARD) use which reduced the observed difference between treatments. By applying a prespecified trial emulation protocol to observational clinical registry data, we could replicate the results of SWEFOT, favoring infliximab over SSZ + HCQ combination therapy at 9 months.
2D Covalent Organic Frameworks Toward Efficient Photocatalytic Hydrogen Evolution
Authors: Li Yang , Song Xiaoyu , Zhang Guang , Wang Lei , Liu Yi , Chen Weihua , Chen Long 
Published: June 2022

AbstractEfficiently producing clean energy is of great importance for sustainable development of the environment. Solar‐driven water splitting for H2 evolution has an important role among the renewable energy technologies. Developing high‐performance and cost‐effective photocatalysts is still a critical task before practical application. 2D Covalent organic frameworks (COFs) as photocatalysts have recently attracted widespread interest thanks to their tunable optical bandgaps, tailor‐made functionality, excellent crystallinity, high specific surface area, and good photo‐ and chemical stability. This Review focuses on the representative progress and remaining challenges in 2D COF‐based photocatalysts for hydrogen evolution.