bluuebunny/crossref_metadata_2025_split huggingface dataset:
BIOTRANSFORMATION OF WASTEWATER PRODUCTION OF BAKERY YEAST WITH BIOGAS GENERATION
Authors: Bublienko Natalia , Zakharova Ruslana , Stetsenko Natalia 
Published: July 2022

Baking yeast enterprises are a source of environmental pollution by concentrated wastewater. They are usually diluted with water and discharged into sewerage. It is rational to use methane fermentation, which will ensure the removal of pollutants and make the process cost-effective through the use of biogas and digestion. Therefore, it is relevant to study the question of the influence of fermentation parameters on the cleaning efficiency.
The aim of the work is to study the methane fermentation of yeast production effluents in a continuous mode. Objectives: analysis of solving the problem of wastewater treatment of yeast plants, study of the influence of process parameters (dilution rate, addition of cobalt salts) on the efficiency of treatment; gas generation; vitamin production.
Initial COD of effluents 4500 mg O2/dm3, pH 6. Cultivation regime – continuous, dilution rate 4,1·10–3, 6,2·10–3, 8,2·10–3, 12,4·10–3 hours–1. Cleaning efficiency 78,9 %. High values ​​of dilution rate cause overload of activated sludge, which leads to reduced cleaning efficiency. The presence of cobalt also has a depressant effect. A significant amount of biogas (up to 5,2 dm3/dm3) is produced with a high content of methane (up to 85 %), which is an alternative fuel. As the dilution rate increases, the biogas and methane content decrease. With increasing dilution rate from 4,1·10–3 to 12,4·10–3 hours–1, biogas decreased from 1,11 to 0,94 dm3/g CODloading, and from 1,43 to 1,39 dm3/g CODfermentation. Similarly to the effect on the depth of purification, the inhibitory effect of cobalt on methanogeneration is observed. Digestion is a valuable fertilizer with a significant content of cobalamin vitamins (up to 95 μсg/g). The addition of cobalt salts stimulates the synthesis of vitamins, providing an increase of 26,7 to 51,6 %, improving the ratio between active and inactive forms.
EXPERIMENTAL SCALING POTENTIAL OF HEATED WATER
Authors: Kontsevoi Serhii , Kontsevoi Andrii , Khokhotva Oleksandr 
Published: July 2022

The previously proposed mechanism of bicarbonate ions decomposition by the H-mechanism (with the formation of H+ and CO32-) or OH-mechanism (with the formation of OH- and CO2) is confirmed experimentally without and with heating water up to 190oC. The change decomposition mechanism depends on hardness, alkalinity and pH. The H-mechanism is observed at higher pH values (pH decreases) and the OH-mechanism is observed at lower values (pH increases) in particular solutions.
The developed technique is based on the measurement of changing the pH (ΔрНt) of the solution (at a fixed temperature of 15 to 25oC) after heating it to a given temperature (ranging from 40 to 190oC) and maintaining this temperature for at least 30 minutes. A decrease in the pH of the water after heating (ΔрНt>0) indicates the formation of carbonate ions and the need for additional water treatment to reduce the hardness, alkalinity or pH. The greater the ΔрНt, the greater the concentration of carbonate ions formed in water. If ΔрНt is zero then this temperature can be used as a water quality index. This temperature is 86.4oC for tap water in Kyiv (pH 7.4). Measurement of ΔрН in water meeting standards for water in German heating supply systems (pH 9.06, Hardness 45 μmol/dm3 - 5 times less than in Ukraine) shows that such water is not scale-safe (ΔрН150=0.15).
The proposed technique will be able to replace the Langelier index (up to 90oC) and the carbonate index (up to 190oC) under the condition of systematic processing of experimental data obtained for the water of different compositions on its basis. This method can be used in addition to the expensive experimental method of dynamic tube blocking.
Application of screening techniques for early diagnostics of bipolar afapplication of screening techniques for early diagnosis of a risk of bipolar disorder in adolescents
Authors: Osipova N. N. , Bardenshteyn L. M. , Lyamets L. L. , Dmitrieva E. V. 
Published: July 2022

Aim. To assess the possibility of combined application of screening methods for early detection of risks of bipolar disorder in adolescents.Materials and methods. The study included 139 adolescents aged 13–16 years. A clinical psychopathology assessment as well as screening methods were used. The screening methods included the Bipolar Spectrum Diagnostic Scale ((BSDS), R. Pies, 2005) and the Mood Disorder Questionnaire ((MDQ), R.M. Hirschfeld, 2000).Results. The clinical psychopathology assessment was performed in accordance with criteria of ICD-10, Class V. No mental and behavioral disorders (F00-F99), including affective pathology, were identified. Following the MDQ screening, the risk of bipolar disorder was revealed in 63 individuals (45.3%; 95 % confidence interval (CI): (36.8–53.9). When the BSDS method was used, the risk of bipolar disorder was revealed in 16.2% of cases (CI: (11.9–28.3)). The combined use of the screening scales (MDQ and BSDS) confirmed their consistency in detecting values both not exceeding (48.7% of the cases) and exceeding the threshold rates (17.1% of the cases).Conclusion. Early diagnosis of a risk of bipolar disorder in adolescents, along with a clinical psychopathology assessment, may include application of screening scales. Сombined use of several screening methods is justified by polymorphism of initial hypomanic and depressive states, as well as by difficulties in subjective assessment of symptoms of bipolar disorder in adolescents.
Insulin-like growth factors and their transporter proteins in the liver of rats with experimental diabetes, adenocarcinoma of the uterine corpus, and their combination
Authors: Frantsiyants E. M. , Bandovkina V. A. , Kaplieva I. V. , Sheiko E. A. , Surikova E. I. , Neskubina I. V. , Trepitaki I. K. , Pogorelova Yu. A. , Cheryarina N. D. , Nemashkalova L. A. , Kotieva I. M. , Shaposhnikov A. V. , Morozova M. I. 
Published: July 2022

Aim. To investigate the content of insulin-like growth factor (IGF)-1, IGF-2, and their transporter proteins IGFBP-1 and IGFBP in the liver of rats with experimental diabetes, Guerin’s carcinoma, and their combination.Materials and methods. The experiment was carried out on 64 white outbred rats of both sexes, which were divided into 4 groups of 8 animals each: group 1 – intact animals, group 2 – animals with experimental diabetes, group 3 – animals with subcutaneously inoculated Guerin’s carcinoma, group 4 – animals with experimental diabetes and subcutaneously inoculated Guerin’s carcinoma. In the study, biochemical and statistical analyses and enzyme immunoassays were performed.Results. In the liver of the outbred rats, sex specificity in the content of insulin-like growth factors and IGFBP-1 was established: the levels of IGF-1, IGF-2, and IGFBP-1 in males were lower than in females. It was shown that the development of diabetes mellitus and the growth of Guerin’s carcinoma led to changes in the sex-specific components in the rat liver.Conclusion. The growth of Guerin’s carcinoma and the progression of diabetes mellitus cause multidirectional changes in IGF and IGFBP levels in the liver of females and unidirectional changes in the liver of males. Following the growth of Guerin’s carcinoma against the background of diabetes mellitus, sex-specific differences in the content of the studied parameters were minimized. It was shown that diabetes mellitus changed the metabolic profile of the liver in the animals of both sexes.
Post-COVID syndrome is associated with increased extracellular purine bases and neutrophil extracellular traps in the blood plasma
Authors: Kazimirskii A. N. , Salmasi J. M. , Poryadin G. V. , Panina M. I. , Larina V. N. , Ryzhikh A. A. 
Published: July 2022

Post-COVID syndrome is characterized by fatigue, reduced exercise tolerance, muscle and joint pain, and psychoemotional disorders. In the development of a generalized body response in a viral infection, abnormal defense responses are of great importance. We studied neutrophils, neutrophil extracellular traps (NETs), DNA degradation products (purine nitrogenous bases, PNBs), and traditional biochemical parameters.Aim. To determine biochemical parameters and the number of NETs and PNBs in the peripheral blood of patients with post-COVID syndrome.Materials and methods. The study included outpatients (n = 21) aged 18–59 years (36 [27 ÷ 50]). The control group consisted of 20 individuals aged 18–59 years (38.5 [29 ÷ 51.5]) without a past medical history of the coronavirus infection. All patients underwent a physical examination, their medical history was assessed, and the level of NETs and PNBs in the venous blood was determined.Results. 11 patients had a mild form of the disease in their past medical history, 7 – moderate, and 3 – severe. The most common symptoms in the patients were fatigue, headache, epigastric pain, dizziness, and joint pain. Hair loss and dyspnea were less common. The concentration of NETs and PNBs was higher in the patients with post-COVID syndrome than in the control group (p < 0.05). We detected NETs in the patients with post-COVID syndrome only in the form of filamentous structures. The concentration of extracellular purine bases in the blood of the patients with post-COVID syndrome was the highest in patients with moderate and severe acute periods. In patients with a mild acute period, the concentration of PNBs was 7.38 [0.0 ÷ 60.7] mg / ml, and in patients with moderate and severe acute periods – 19.15 [0.0 ÷ 33.5] and 34.19 [3.35 ÷ 70.0] mg / ml, respectively.Conclusion. Extracellular purine bases in concentrations capable of causing secondary alteration of cells are found in the peripheral blood of patients with post-COVID syndrome. Post-COVID syndrome is accompanied by the formation of filamentous NETs in the blood of patients.
Interferons alpha and gamma, pidotimod, and tilorone in the treatment of acute respiratory infections in patients with allergic rhinitis: a prospective, cohort clinical and immunological study
Authors: Kalyuzhin O. V. , Ponezheva L. O. , Turapova A. N. , Nurtazina A. Yu. , Bykov A. S. , Karaulov A. V. 
Published: July 2022

Aim. To compare the clinical efficacy and influence on interferon (IFN) production / sensing of drugs with immunemediated antiviral effects, which potentiate type 1 (T1) immune responses, in the treatment of acute respiratory infections (ARI) in patients with allergic rhinitis.Materials and methods.146 ARI patients with remission of seasonal allergic rhinitis were divided into 4 cohorts. In addition to symptomatic therapy, patients received either 2,000 IU of IFNγ in each nasal passage 5 times a day; or rectal suppositories containing 106 IU of IFN-α2b and antioxidants (AO) twice a day, and a gel with IFN-α2b and AO intranasally 3 times a day; or 400 mg of pidotimod per os twice a day; or 125 mg of tilorone per os on days 1, 2, 4, and 6. The severity of ARI was determined daily as the sum of 10-point scores for 15 symptoms. Serum concentrations of IFNα and IFNγ and the ability of blood cells to produce these cytokines ex vivo spontaneously and upon stimulation with Newcastle disease virus or phytohemagglutinin were studied using enzyme-linked immunosorbent assay (ELISA). The proportions of circulating lymphocytes expressing type I IFN receptor subunit 2 (CD118) or IFNγ receptor α-chain (CD119) were determined by flow cytometry.Results. ARI symptoms in all cohorts generally regressed in a similar way. However, from day 5 of the treatment, pidotimod relieved symptoms more effectively than other drugs. In patients treated with tilorone, the regression of ARI manifestations was delayed in the first two to three days, followed by rapid symptom reduction. An initial decrease in the induced production of IFNγ was found in patients treated with pidotimod, and a tendency to a decrease in this parameter was noted in other cohorts. The induced production of IFNγ after the treatment in all groups did not differ from that in healthy donors. No significant changes and differences in the proportions of CD118+ and CD119+ lymphocytes were found between the cohorts, except for a decrease in the number of CD118+ cells after the treatment with tilorone. In patients treated with IFN-α2b + AO, the proportions of CD119+ and CD118+ lymphocytes tended to increase slightly.Conclusion. Drugs that promote the development of T1 over T2 immune responses are a useful option for treating ARI in patients with allergic rhinitis.
Land use change in Baghdad City and assessment of the Jadriyah and Umm Al- Khanazeer Island Important Bird Area (IBA) from 1984 to 2020
Authors: Fazaa Nadheer A. , Ali Abdulrahman B. , AL-Jabinawi Ahmad JM , Francksen Richard , Whittingham Mark J. 
Published: July 2022

Land use change, particularly the expansion of urban areas and associated human activities at the expense of natural and semi-natural areas, is a major ecological issue in urban areas around the world. Climate change being a very strong additional driver for changing the temperature and habitat in the cities. This also applies to Baghdad, Iraq, where urbanisation and climate change exerts a major pressure on the natural habitats of the city, and thus may affect the ability of city planners to adapt to future climate change scenarios. Here we present evidence of substantial growth in urban areas, increases in temperature, and degradation of natural vegetation within Baghdad city by using Remote Sensing techniques and an assessment for the Jadriyah and Umm Al-Khanazeer site (JUKI). These changes were associated with loss of bird species richness within the area, which was previously the only Important Bird Area (IBA) within the city. A standardised scoring system (following Birdlife International global framework) was used to assess Pressure-State-Response: JUKI site scored 3-5 for pressure (Medium), two for the state (Moderate), and two for the response (Low). Despite the degradation highlighted in Baghdad city, the JUKI site still has 88% intact habitat to support bird trigger species. We conclude that the site urgently needs a detailed management plan to ensure the protection of its habitats and avian fauna, and that the area should be declared as a protected area according to the “IUCN Category IV: Habitat/Species Management Area; to provide a means by which the urban residents may obtain regular contact with nature”, and re-designated JUKI as an IBA site. The study also identifies the most affected areas in the city of Baghdad, which should take the priority of the afforestation efforts and any future restoration campaigns.
Cutaneous small vessel vasculitis associated to palpable purpura after Covid 19 Vaccination: a case report and a literature review
Authors: tirotta daniela , Girelli Francesco , tassinari maurizio , Mazzeo Vincenzo , Muratori Paolo 
Published: July 2022

Abstract
Covid-19 vaccination is mostly safe and effective: adverse events to vaccinations are usually mild and well tolerated. These adverse reactions may include cutaneous reactions and, rarely, cutaneous small vessel vasculitis associated to palpable purpura.We present a case of a small-vessel vasculitis after covid-19 vaccination, that manifested without evidence organ involvement and systemic symptoms, associated to self-resolution, and a review of the cases described in literatureWe have collected the epidemiological and clinical features of these cases.
Effects of cranberry powder on the diversity of microbial communities and quality characteristics of fermented sausage
Authors: Yang Le , Yan Xinlei , Liu Ting , Kang Letian , Gao Xingyu , Sun Yufei , Duan Yan 
Published: July 2022

Abstract
Fermented sausage is popular with many consumers because of its distinctive flavor, but the safety of it has attracted widespread attention. At present, nitrite is widely used in fermented meat products because of its ideal color and bacteriostatic effect, but nitrite can be transformed into nitrosamines, which cause strong carcinogenic effects. Therefore, it is urgent to actively explore safe and efficient nitrite substitutes. In this study, cranberry powder was selected as a natural substitute for nitrite during the production of fermented sausage due to its unique antioxidant and bacteriostatic properties. The results showed that adding an appropriate amount of cranberry powder (5 g/kg) promoted a better color of the fermented sausage and promoted the accumulation of aromatic compounds. Furthermore, Pediococcus and Staphylococcus became the dominant species, accounting for more than 90% in all samples. According to the Pearson correlation analysis, Staphylococcus and Pediococcus had positive effects on the quality characteristics of fermented sausage products. This study provided the latest information on the application of cranberry powder as a natural substitute for nitrite in the process of manufacturing fermented sausage, and it also introduced an advanced solution to improve the quality characteristics and safety of fermented sausage products during processing.
Quantum-Classical Computational Molecular Design of Deuterated High-Efficiency OLED Emitters
Authors: Jones Gavin , Gao Qi , Kobayashi Takao , Sugawara Michihiko , Yamashita Hiroki , Kawaguchi Hideaki , Tanaka Shu , Yamamoto Naoki 
Published: July 2022

Abstract
This study describes a hybrid quantum-classical computational approach for designing synthesizable deuterated Alq3 emitters possessing desirable emission quantum efficiencies (QEs). This design process has been performed on the tris(8-hydroxyquinolinato) ligands typically bound to aluminum in Alq3 and involves optimization of the hydrogen/deuterium (H/D) ratios in these molecules for the discovery of optimally deuterated high-efficiency OLED emitters. It involves a multi-pronged approach which first utilizes classical quantum chemistry to predict the emission QEs of the $Alq_3$ ligands. These initial results were then used as a machine learning dataset for a factorization-machine-based model which was applied to construct an Ising Hamiltonian to predict emission quantum efficiencies on a classical computer. We show that such a factorization machine-based approach can yield accurate property predictions for all 64 deuterated $Alq_3$ emitters with 13 training values. Moreover, another Ising Hamiltonian could be constructed by including synthetic constraints which could be used to perform optimizations on a quantum simulator and device using the variational quantum eigensolver (VQE) and quantum approximate optimization algorithm (QAOA) to discover a molecule possessing the optimal QE and synthetic cost. We observe that both VQE and QAOA can correctly predict the optimal molecule with greater than 0.95 probability on quantum simulators. These probabilities decrease to 0.83 and 0.075 for simulations with VQE and QAOA, respectively, on a quantum device, while the application of the readout error mitigation technique only improves probabilities to 0.90 and 0.084. We have developed the Recursive Probabilistic Variable Elimination (RPVE) method for simulations on noisy devices that recursively eliminates qubits depending on the probability that each qubit possesses a binary value and have demonstrated that RPVE improves the accuracies of VQE and QAOA for predictions of optimal solutions with probabilities of 0.97 on a quantum device.
Research progress on pharmacological actions and molecular mechanisms of Anemoside B4
Authors: Lu Tianming , Wang Qixin , Li Haiyan , Zhao Minghong , Guo Qiuyan , Wang Jigang 
Published: July 2022

Abstract
Anemoside B4 (AB4), as an important ingredient of Chinese herb Pulsatilla Chinensis, has been used in Chinese ancient medicine for thousands of years with exact therapeutic effects of anti-inflammation and anti-tumor. Due to its high potency and low toxicity, AB4 has caused more and more attention of researchers. In this paper, we will first review the clinical usage and fundamental research of AB4 against damaging inflammation of liver, lung, kidney, intestine and joints. Then, we will overview its application and molecular mechanisms in a range of cancers, such as liver cancer, lung cancer, colorectal cancer and cervical cancer. In addition, we pay attention to its pharmacological actions and molecular mechanisms against other diseases, for example, retinal injury, allergic conjunctivitis, pain, vulvovaginal inflammatory disease, acute infectious disease and oral ulcer. Finally, we will discuss the future perspectives of AB4, so as to improve its wide application in clinic.
Can R&amp;D input reduce carbon emissions intensity? Evidence from China
Authors: Zhao Yan , Sun Hui , Xia Xuechao , Ma Dianyuan 
Published: July 2022

Abstract
Climate change caused by greenhouse gas emissions has received widespread attention in recent years. In this paper, panel data of 30 Chinese provinces from 2007 to 2019 are used as research samples to explore research and development input (RDI) on carbon emission intensity (CEI). They are using spatial measurement method, interaction effect model, and mediation effect model, this paper studies whether RDI reduces China's CEI. The empirical results illustrate a positive spatial autocorrelation between RDI and CEI. The spatial agglomeration characteristics of CEI have apparent regional differences. RDI can reduce not only the local CEI, but also the CEI of surrounding areas, and the results are robust. The market and government play an important role in RDI that affects CEI, but there are regional differences. At the same time, RDI can indirectly reduce CEI by promoting industrial upgrading, improving technological innovation, and increasing human capital.
Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1
Authors: Li Yihong , Cai Ting , Liu Huina , Lei Yun , Zhang Shun , Sun Fuyu , Liu Jie , Chen Shao-yu , Fan Huadong 
Published: July 2022

Abstract
During embryonic development, two populations of multipotent stem cells, cranial neural crest cells (NCCs) and epibranchial placode cells (PCs), are anatomically adjacent to each other. The coordinated migration of NCCs and PCs plays a major role in the morphogenesis of craniofacial skeletons and cranial nerves. It’s known that ethanol-induced dysfunction of NCCs and PCs is a key contributor to the defects of craniofacial skeletons and cranial nerves implicated in Fetal Alcohol Spectrum Disorder (FASD). However, how ethanol disrupts the coordinated interaction between NCCs and PCs was not elucidated. To fill in this gap, we established a well-designed cell co-culture system to investigate the reciprocal interaction between human NCCs (hNCCs) and human PCs (hPCs), and also monitored the migration behavior of NCCs and PCs in zebrafish embryos. We found that ethanol exposure resulted in a disruption of coordinated hNCCs-hPCs interaction, as well as in zebrafish embryos. Treating hNCCs-hPCs with exosomes derived from ethanol-exposed hNCCs (Exo EtOH ) mimicked ethanol-induced impairment of hNCCs-hPCs interaction. We also observed that a chemoattractant, SDF1, was downregulated in ethanol-treated hPCs and zebrafish embryos. Meanwhile, miR-126 level in Exo EtOH was significantly higher than that in control exosomes (Exo Con ). We further validated that Exo EtOH -encapsulated miR-126 from hNCCs can be transferred to hPCs to suppress SDF1 expression in hPCs. Knockdown of SDF1 replicated ethanol-induced abnormalities either in vitro or in zebrafish embryos. On the contrary, overexpression of SDF1 or inhibiting miR-126 strongly rescued ethanol-induced impairment of hNCCs-hPCs interaction and developmental defects.