bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research methodology for industry‐academic collaboration – a case study
Authors: Ali Haytham B. , Langen Tommy , Falk Kristin 
Published: September 2022

AbstractWhen industry and academia collaborate within an applied sociotechnical research project, it generates a complex context due to different means, goals, languages, and emergent practices. The industry is interested in developing projects, maximizing profit, and satisfying its clients and customers. Academia is oriented towards performing research to understand, explore, optimize, and verify meaningful statements. Different goals, timelines, ways of working, languages, and resources create too many emergent practices and friction in the research execution. In this paper, we perform a retrospective analysis on a research project conducted over a year that focused on the current and future state in nine companies and evaluated its use of research methodologies. A finding is the need to have well‐defined industry cases, and we suggest criteria for achieving this. Additionally, we see emergent practices and dynamic environments influencing the research process in industry and academic research context.
A Conceptual Model‐Based Systems Engineering Method for Creating Secure Cyber‐Physical Systems
Authors: Larsen Martin H. , Muller Gerrit , Kokkula Satyanarayana 
Published: September 2022

AbstractThe Air Traffic Control industry is being increasingly exposed to rising levels of risk, as criminals and cyber‐attackers look to exploit system vulnerabilities. Air Navigation Service Providers become more demanding regarding cybersecurity concerns in the products they acquire. Consequently, systems engineers need to consider cyber security concerns early in their system's development life cycle. Model‐Based Systems Engineering methodologies are widely used to manage complex engineering projects in terms of system requirements, design, analysis, verification, and validation activities, leaving cyber security aspects aside. This paper presents a conceptual solution of a model‐based security method that aims to enable systems engineers to perform threat modeling analysis of cyber‐physical systems early and incorporate mitigation strategies into the system design, thereby reducing the cyber‐physical system's overall security‐related risks. Based on a real‐life case study the method will be validated later during execution period from Jan. – May 2022.
Maternal age and body mass index as risk factors for rectovaginal colonization with group B streptococci
Authors: Khalil Mohammed R. , Hartvigsen Camilla M. , Thorsen Poul B. , Møller Jens K. , Uldbjerg Niels 
Published: September 2022

AbstractObjectiveTo examine the effect of including maternal age and body mass index (BMI; calculated as weight in kilograms divided by the square of height in meters) as additional risk factors in the traditional risk‐based strategy at term pregnancies consisting of previous early‐onset group B streptococcus (GBS) disease, GBS bacteriuria during pregnancy, maternal temperature of 38.0°C or more intrapartum, and rupture of membranes of 18 h or longer.MethodsA secondary analysis of a Danish cohort including 902 pregnant women. Exposures were maternal age and pre‐pregnancy BMI. Outcome was rectovaginal GBS colonization at the time of labor. The logistic regression analysis adjusted for parity, gestational age, vaginal delivery, and smoking.ResultsThe GBS prevalence was 17% in the entire population, 35% among participants older than 40 years, and 23% among those with a BMI of 25 or greater. Including maternal “age > 40” as an additional risk factor increased the sensitivity of the risk‐based strategy from 21% to 26% and decreased the specificity from 90% to 87%. Inclusion of “BMI ≥ 25” increased the sensitivity from 21% to 57% and decreased the specificity from 90% to 59%.ConclusionsMaternal age and BMI might be included as additional risk factors in risk‐based programs for identification of GBS‐positive laboring women to receive intrapartum antibiotics prophylaxis.
Determinants of menstrual cup use among undergraduate medical students: A cross‐sectional study
Authors: Czekalski Lobascz Brenda , Reis Maria Beatriz de França , Mendes Gabriella de Perez Monteiro e Tiburcio , Cruz Danillo Christian de Oliveira , Damaceno Maria Jose Caetano Ferreira , Sartorao Filho Carlos Izaias 
Published: September 2022

AbstractObjectiveTo identify the determinants of menstrual cup use among medical students in Brazil.MethodsA cross‐sectional study with an online survey regularly applied to matriculate women from Medical School in January 2021.ResultsOf 277 women, 164 participated. The mean age was 22.26 ± 3.21 years. In all 136 women preferred external pads, 60 internal pads, 28 menstrual cups, and 11 did not use. Using a 1–10 scale, price, efficacy, sustainability, practice, vaginal health status, hygiene, and importance of internal genital integrity were investigated. Thirty‐seven (22.56%) women related the use of menstrual cups in the last year. Using any method to reduce menstruation had an adjusted odds ratio (aOR) of 0.310 (95% confidence interval [CI] 0.122–0.787). Concern about the environmental biodegradation had an aOR of 6.369 (95% CI 1.372–29.562); concern about intimal health had an aOR 1.996 (95% CI 1.183–3.368); and concern about internal genital integrity had an aOR of 0.824 (95% CI 0.682–0.995) for menstrual cup use.ConclusionThe significant independent factors for the use or adherence to menstrual cups were: women using a method to reduce their menstrual flow; concerning biodegradation; concerning vaginal health; with no concerns about manipulating their genitalia. The menstrual cup was used by 22.56% of a medical school in Brazil among undergraduate women.
Surgical complications and their impact for gynecological surgeons
Authors: Chauvet Pauline , Figuier Claire , Lambert Céline , Bourdel Nicolas , Canis Michel 
Published: September 2022

AbstractObjectivesMajor clinical incidents can impact the healthcare professionals involved. This is of particular relevance in surgery, with the operating room being a high‐risk zone for complications; however, there is few available data on how surgeons may be affected. The current study examined the impact of surgical complications on surgeon traumatic stress levels, emotional state, job performance, and coping strategies.MethodsA questionnaire to evaluate the impact of surgical complications on surgeon traumatic stress levels, emotional state, and job performance was developed by our team and communicated via an online link to gynecological surgeons.ResultsA total of 72 gynecologic surgeons completed the questionnaire. Five percent had a Peritraumatic Distress Inventory (PDI) score of ≥15, revealing a high stress level, and 12% had an Impact of Event Scale–Revised (IES‐R) score of ≥36, indicating acute traumatic stress. Our results show that following surgical incidents, surgeons receive support primarily from another surgical team member and that surgical practice may be impacted, leading in some cases to detrimental effects on patient care, notably reduced radicality in some surgical procedures.ConclusionSurgeons may experience acute traumatic stress after serious surgical complications. Increased awareness of the negative consequences on surgeon emotional well‐being is required, as well as improved access to support mechanisms.The study was approved by local ethics committee (IRB00013412, “CHU de Clermont Ferrand IRB #1,” institutional review board number 2022‐CF004).
Single‐shot optical projection tomography for high‐speed volumetric imaging of dynamic biological samples
Authors: Darling Connor , Davis Samuel P. X. , Kumar Sunil , French Paul M. W. , McGinty James 
Published: September 2022

AbstractA single‐shot adaptation of Optical Projection Tomography (OPT) for high‐speed volumetric snapshot imaging of dynamic mesoscopic biological samples is presented. Conventional OPT has been applied to in vivo imaging of animal models such as D. rerio, but the sequential acquisition of projection images typically requires samples to be immobilized during the acquisition. A proof‐of‐principle system capable of single‐shot tomography of a ~1 mm3 volume is presented, demonstrating camera‐limited rates of up to 62.5 volumes/s, which has been applied to 3D imaging of a freely swimming zebrafish embryo. This is achieved by recording eight projection views simultaneously on four low‐cost CMOS cameras. With no stage required to rotate the sample, this single‐shot OPT system can be implemented with a component cost of under £5000. The system design can be adapted to different sized fields of view and may be applied to a broad range of dynamic samples, including high throughput flow cytometry applied to model organisms and fluid dynamics studies.
miR‐208 inhibits myocardial tissues apoptosis in mice with acute myocardial infarction by targeting inhibition of PDCD4
Authors: Wang Qiang , Zhou Huxiang , Zhu Xiaobo , Jiang Feng , Yu Qiuhua , Zhang Junjie , Ji Yaxiang 
Published: September 2022

AbstractThis article aimed to investigate the role of miR‐208 in the apoptosis of myocardial tissues in acute myocardial infarction (AMI) mice. The AMI mouse model was constructed. Then, miR‐208 expression in AMI mice was regulated by transfection. The mouse myocardial tissues were subject to hematoxylin–eosin (HE) staining, TUNEL assay, and immunofluorescence analysis. H9c2 cell transfection and hypoxia induction were then completed, and cell apoptosis and cytokine levels were tested. Additionally, RNA pull‐down and dual luciferase reporter gene assays were conducted for exploring the relation of miR‐208 with programmed cell death 4 (PDCD4). Additionally, fluorescence in situ hybridization (FISH) was conducted for investigating miR‐208 and PDCD4 colocalization within H9c2 cells. AMI mice had severe damage, apoptosis, decreased miR‐208 expression, increased IL‐1β, IL‐6, IL‐8 levels, whereas reduced IL‐10 level within myocardial tissues. H9c2 cells under hypoxia induction exhibited decreased miR‐208 expression, promoted apoptosis, increased protein expression of Bax and cleaved‐caspase‐3, decreased protein expression of Bcl‐2 and caspase‐3, elevated IL‐1β, IL‐6, IL‐8 levels and decreased IL‐10 level. miR‐208 upregulation alleviated the damage and apoptosis of myocardial tissues in AMI mice. AMI mice with miR‐208 upregulation showed decreased expression of Bax and cleaved‐caspase‐3, increased expression of Bcl‐2 and caspase‐3, reduced levels of IL‐1β, IL‐6, IL‐8, whereas an increased level of IL‐10. miR‐208 showed direct inhibition of PDCD4. PDCD4 and miR‐208 were mainly co‐expressed in the cytoplasm. The upregulated PDCD4 expression abolished miR‐208's suppression of H9c2 cell apoptosis induced by hypoxia. Besides this, miR‐208 inhibited myocardial tissue apoptosis in AMI mice by inhibiting PDCD4 expression.
Antiosteoporosis and bone protective effect of nimbolide in steroid‐induced osteoporosis rats
Authors: Huo Jiang , Ding Yu , Wei Xinyuan , Chen Qi , Zhao Bin 
Published: September 2022

AbstractBackgroundOsteoporosis is a metabolic, hereditary, progressive disease characterized by unusual bone production across the skeleton and a loss the bone tissue microstructure and mass. In this experimental study, we scrutinized the antiosteoporosis effect of nimbolide against glucocorticoid (GCs) induced osteoporosis in rats.MethodsSwiss albino female rats were employed for the current experiment study and the rats were divided into different groups. Dexamethasone (0.1 mg/kg/day) was used for induction the osteoporosis and the rats were received the different doses of nimbolide (2.5, 5, and 7.5 mg/kg) for the estimation of bone protective effects. The body weight was estimated (initially and finally). Hormones, bone metabolic markers, bone turnover markers, bone structure, biomechanical, histomorphometric dynamic, biochemical markers, and histomorphometric static parameters were analyzed.ResultsThe body weight of GCs group rats considerably suppressed and nimbolide treatment remarkably improved the body weight. Nimbolide treated group exhibited the enhancement of bone metabolic, bone structure markers, and histomophometric dynamic markers, which was suppressed during the GCs‐induced osteoporosis. GCs‐induced osteoporosis rats exhibited the enhancement of procollagen type 1 C‐terminal propeptide (P1CP), carboxy‐terminal crosslinked telopeptide of type 1 collagen (CTX‐1), Dickkopf‐1 (DKK1), tartrate‐resistant acid phosphatase 5b (TRACP 5b), and suppressed the level of bone alkaline phosphatase (BAP), which was reversed by the nimbolide treatment. Nimbolide treatment remarkably improved the level of estradiol and suppressed the level of parathyroid hormone (PTH), which was altered during the osteoporosis. Nimbolide treatment significantly (p < 0.001) improved the level of calcium, magnesium, and phosphorus in the serum and bone tissue. Nimbolide treatment also altered the level of bone metabolic markers and suppressed the level of inflammatory cytokines.ConclusionBased on the findings, we may conclude that nimbolide has antiosteoporosis properties via balancing the bone mass and improving vitamin and hormone levels.
Bone marrow free immune checkpoints as a potential biomarker for differential diagnosis of acquired bone marrow failures
Authors: Zang Mengtong , Li Nianbin , Chen Qiulin , Ran NingYuan , Fu Rong , Shao Zonghong , Wang Ting 
Published: September 2022

AbstractObjectiveClinically, to make a definite diagnosis of aplastic anemia (AA), idiopathic cytopenia of undetermined significance (ICUS) or myelodysplastic syndrome (MDS), they should be distinguished from each other. AA and ICUS have some incidence to transform into MDS. Immunosuppressive therapy (IST) is effective in AA and partial ICUS patients, while other ICUSs are more likely to progress to MDS without response to IST. To date, we neither found a technical method that could easily identify AA from hypoproliferative MDS, nor a simple parameter that could indicate ICUS with a response to IST. Here, we detected the concentration of free immune checkpoints in bone marrow supernatant of AA, ICUS, and MDS patients, analyzed the differences of immune status among these three diseases, to try to find a way to predict the response to IST in ICUSs.MethodsSeventy‐four novel patients were enrolled with newly diagnosed acquired bone marrow failure (including 29 AA patients, 11 ICUS patients, and 34 MDS patients), bone marrow supernatants were collected. Luminex liquid suspension array technology was used to measure the concentrations of 17 immune checkpoints to analyze the differences of immune status among these three diseases.ResultsThe levels of 17 free immune checkpoints were elevated in MDS and showed a strong correlation with each other, followed by ICUS, and with the weakest in AA. By drawing the ROC curve, we found eight immune checkpoints, including sCD40, sCD86/B7‐2, sCTLA‐4, sGITR, sHVEM, sPD‐1, sTIM‐3, and sTLR‐2, could easily distinguish AA from hypoproliferative MDS. ICUSs with lower concentrations of these eight free immune checkpoints predicted a better IST response.ConclusionIn conclusion, we found that there were notable differences in the immune status of AA, ICUS, and MDS. The concentrations of sCD40, sCD86/B7‐2, sCTLA‐4, sGITR, sHVEM, sPD‐1, sTIM‐3, and sTLR‐2 could be used to identify AA and hypoproliferative MDS patients, as well as to distinguish ICUS patients who could benefit from IST.
The DNAm levels of CREB5 (cg11301281) were associated with clopidogrel resistance
Authors: Li Jiyi , Yang Jin , Yu Qinglin , Chen Lian , Shi Xiliang , Su Jia , Zhu Keqi 
Published: September 2022

AbstractPurposeClopidogrel resistance (CR) is mostly caused by interindividual variability of the platelet inhibition of clopidogrel, which may induce cardiovascular events. The aim of this research was to evaluate whether DNAm levels of CREB5 (cg01534253) are involved in CR among acute coronary syndrome (ACS) patients treated with clopidogrel.Methods72 patients(36 CR and 36 non‐CR) who underwent ACS were included in this study. The VerifyNow P2Y12 assay was selected to evaluate residual platelet reactivity, and bisulfite pyrosequencing methods was used to examine DNA methylation levels on cg01534253. Secondly, CREB5 mRNA expression was analyzed via quantitative real‐time PCR. Last, we employed logistic regression to test the interaction between genetic factors of CREB5 methylation and multiple clinical variables in CR patients.ResultsSubunit analysis indicated that for patients whose HbA1c levels were ≥6.5% or whose GLU levels were ≥7 mmol/L, lower methylation of cg01534253 indicated a poorer clopidogrel response. In addition, CREB5 mRNA expression was increased in CR patients with GLU levels ≥7 mmol/L. Moreover, regression analysis indicated that the values of albumin and uric acid were correlated with the incidence of CR.ConclusionsOur findings were likely to provide fresh understanding for the new mechanism of platelet inhibition failure and promote individualized antiplatelet therapy.
Sclerostin aggravates insulin signaling in skeletal muscle and hepatic steatosis via upregulation of ER stress by mTOR‐mediated inhibition of autophagy under hyperlipidemic conditions
Authors: Oh Heeseung , Park Seung Yeon , Cho Wonjun , Abd El‐Aty A. M. , Hacimuftuoglu Ahmet , Kwon Chang Hyuk , Jeong Ji Hoon , Jung Tae Woo 
Published: September 2022

AbstractRecently, sclerostin (SCL), a circulating glycoprotein, was proposed to be a novel myokine involved in developing metabolic disorders. The association between SCL levels and insulin resistance in skeletal muscle, liver, and adipose tissue was studied in individuals with aggravated glucose tolerance. Thus, we hypothesized that elevated circulating SCL might affect skeletal muscle insulin signaling and hepatic lipid metabolism, and aimed to investigate the effects of SCL on skeletal muscle insulin resistance and hepatic steatosis in obesity using in vitro and in vivo experimental models under hyperlipidemic conditions. In the current study, we found elevated SCL messenger RNA expression levels in myocytes in obese patients. In addition to a higher blood level, SCL was expressed at an elevated level in the skeletal muscle of mice fed a high‐fat diet (HFD). Higher SCL release levels and expression were also noticed in palmitate‐treated C2C12 myocytes. SCL suppression by in vivo transfection improves skeletal muscle insulin resistance and hepatic steatosis in HFD‐fed mice. The treatment of C2C12 myocytes with recombinant SCL aggravated insulin signaling. Furthermore, treatment with SCL augmented lipogenic lipid deposition in human primary hepatocytes. Treatment with SCL upregulated mammalian target of rapamycin (mTOR) phosphorylation and suppressed autophagy markers, thereby causing endoplasmic reticulum (ER) stress. 4‐Phenylbutyric acid, a pharmacological ER stress inhibitor, abolished the effects of SCL on insulin signaling in C2C12 myocytes and lipid accumulation in primary hepatocytes. In conclusion, SCL promotes skeletal muscle insulin resistance and hepatic steatosis by upregulating ER stress via the mTOR/autophagy‐mediated pathway. The present study suggests that antagonizing SCL might be a novel therapeutic strategy for simultaneously managing insulin resistance and hepatic steatosis in obesity.
P‐Glycoprotein and Organic Anion Transporter Polypeptide 1B/Breast Cancer Resistance Protein Drug Transporter Activity in Pregnant Women Living With HIV
Authors: Moreira Fernanda de Lima , Melli Patrícia Pereira dos Santos , Marques Maria Paula , Rocha Adriana , Nardotto Glauco Henrique Balthazar , Duarte Geraldo , Lanchote Vera Lucia 
Published: September 2022

AbstractThis study evaluates the influence of pregnancy and HIV infection in conjunction with the use of raltegravir, lamivudine, and tenofovir disoproxil fumarate (combined antiretroviral therapy [cART]) on intestinal P‐glycoprotein (P‐gp) and hepatic organic anion transporter polypeptide (OATP) 1B1/1B3 and/or breast cancer resistance protein (BCRP) drug transporter activity using rosuvastatin (OATP1B/BCRP) and fexofenadine (P‐gp) probes. Single oral doses of 5‐mg rosuvastatin and 60‐mg fexofenadine were administered to women living with HIV under cART in the third trimester of gestation (n = 15) and postpartum period (n = 10). A control group of 12 healthy nonpregnant women also was investigated. Pharmacokinetic parameters were estimated by using a noncompartmental method and evaluated by t test (P < .05). The rosuvastatin area under the plasma concentration–time curve from time 0 to the last quantifiable concentration (AUC0‐last) value was higher in the third trimester of pregnancy (19.5 [95%CI, 16.8–22.3] ng • h/mL] when compared to postpartum (13.3 [95%CI, 9.3–17.5] ng • h/mL), while the fexofenadine AUC0‐last values did not differ between the third trimester of pregnancy (738.0 [95%CI, 611.4–864.6] ng • h/mL) and postpartum period (874.9 [95%CI, 408.2–1342.0] ng• h/mL). The rosuvastatin AUC0‐last values did not differ between healthy nonpregnant women (13.8 [95%CI, 10.0–17.6] ng • h/mL) and women living with HIV in the postpartum period (13.3 [95%CI, 9.3–17.5] ng • h/mL), and the fexofenadine AUC0‐last values did not differ between the 2 investigated groups (603.6 [95%CI, 467.5–739.7] ng • h/mL vs 874.9 [95%CI, 408.2–1342.0] ng • h/mL). It is suggested that gestation inhibits the hepatic OATP1B1/1B3 and/or BCRP activity but does not alter intestinal P‐gp activity. The influence of HIV infection in conjunction with use of cART on OATP1B/BCRP and intestinal P‐gp activity was not observed.
Evaluation of the Pharmacokinetic Drug Interaction of Capmatinib With Itraconazole and Rifampicin and Potential Impact on Renal Transporters in Healthy Subjects
Authors: Cui Xiaoming , Chen Xinhui , Pognan Nathalie , Sengupta Tirtha , Rahmanzadeh Gholamreza , Kornberger Ruediger , Giovannini Monica 
Published: September 2022

AbstractCapmatinib is a highly specific, potent, and selective mesenchymal–epithelial transition factor inhibitor predominantly eliminated by cytochrome P450 (CYP) 3A4 and aldehyde oxidase. Here, we investigated the effects of a strong CYP3A inhibitor (itraconazole) and a strong CYP3A inducer (rifampicin) on single‐dose pharmacokinetics of capmatinib. In addition, serum creatinine and cystatin C were monitored to assess the potential inhibition of renal transporters by capmatinib. This was an open‐label, 2‐cohort (inhibition and induction), 2‐period (capmatinib alone and inhibition/induction periods) study in healthy subjects. In the inhibition cohort, capmatinib (400 mg/day) was given alone, then with itraconazole (200 mg/day for 10 days, 5‐day lead‐in before coadministration). In the induction cohort, capmatinib (400 mg/day) was given alone, then with rifampicin (600 mg/day for 9 days, 5‐day lead‐in before coadministration). Fifty‐three subjects (inhibition cohort, n = 27; induction cohort, n = 26) were enrolled. Coadministration of itraconazole resulted in an increase of capmatinib area under the plasma concentration–time curve from time 0 to infinity by 42% (geometric mean ratio [GMR], 1.42; 90%CI, 1.33–1.52) with no change in maximum plasma concentration (GMR, 1.03; 90%CI, 0.866–1.22). Coadministration of rifampicin resulted in a reduction of capmatinib area under the plasma concentration–time curve from time 0 to infinity by 66.5% (GMR, 0.335; 90%CI, 0.300–0.374) and a decrease in maximum plasma concentration by 55.9% (GMR, 0.441; 90%CI, 0.387–0.502). After a single dose of capmatinib, a transient increase in serum creatinine was observed with no change in serum cystatin C concentration during the 3‐day monitoring period. In conclusion, coadministration of itraconazole or rifampicin resulted in clinically relevant changes in systemic exposure to capmatinib. The transient increase in serum creatinine without any increase in cystatin C suggests inhibition of renal transport by capmatinib.