bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute effects of high intensity training on cardiac function - A pilot study comparing subjects with type 2 diabetes to healthy controls.
Authors: Ness Henning O , Ljones Kristine , Gjelsvik Randi H , Tjønna Arnt Erik , Malmo Vegard , Nilsen Hans Olav , Hollekim-Strand Siri Marte , Dalen Håvard , Høydal Morten A 
Published: February 2022

Abstract
Purpose. The purpose of this study was to evaluate the acute stress on the heart following one session of high intensity interval training in patients with type 2 diabetes (T2D) versus healthy controls. Methods. High intensity aerobic exercise was performed by 4x4 minutes intervals (90-95% of maximal heart rate), followed by a ramp protocol to peak oxygen uptake (VO2peak). Echocardiography was performed before, and 30 minutes after exercise. Heart rhythm was monitored 24h pre-exercise, during and 24h post exercise by Holter electrocardiogram. Results. After exercise we report a reduction in in both groups in the early diastolic peak mitral annular velocity, the ratio of mitral peak early to late diastolic inflow. Further, left atrial end-systolic volume was reduced after exercise. The LV end-diastolic wall thickness increased in CG. T2D had significant more supraventricular extrasystoles per hour before and after exercise. Conclusion. One single session of exhaustive exercise induced acute cardiac alterations in both left- and right-sided cardiac chambers with reduction in volumes and indices of systolic and diastolic dysfunction in both T2D patients and controls. There was no indication of different stress response in T2D compared to controls. ClinicalTrials.gov Identifier: NCT02998008
Electronic transfusion consent and blood delivering pattern improve the management of blood bank in China
Authors: Jiang Luxi , Zhang Guobing , Hao Ke , Xiang Weiling , Zhang Qin , Xie Yiwei , Du Yaoqiang , Wang Zhen , Chen Bingyu 
Published: February 2022

Abstract
Background

The aim of this study was to improve the accuracy of blood transfusion treatment consent, simplify the verification process, prolong the temperature control time before blood transfusion, and save the labor cost of blood transportation.
Methods

We designed the electronic signing process of blood transfusion consent, which can generate personalized text content and automatically check the accuracy of filling. The signal can be transmitted to the blood transfusion management system (TMS) to relieve the blood distribution. For blood delivering, we established the blood transport center, recruited full-time nurses and used temperature-controlled blood transfer boxes to deliver blood in batches on a regular basis.
Results

Quarterly analysis of blood transfusion quality data showed that the accuracy of blood transfusion consent was 100% after electronic consent process implemented. The average savings of confirmation time between electronic content and papery content was 26min for Department of Emergency (Estimated Difference (95% CI) = 26 (20 to 36), p< 0.05). The pattern of blood delivering reduced the time for each unit of leaving temperature control by 7.24min on average (Estimated Difference (95% CI) = 7.24 (6.92 to 7.56), p< 0.05), and saved $3.67 of labor cost for each unit as well.
Conclusion

Electronic transfusion consent process not only ensures the accuracy, but also saves the verification time. Moreover, the blood delivering pattern prolongs the temperature control time of blood, and saves labor costs. These two improvements could enhance transfusion management.
Alleviation of the adverse effects of NaCl stress on tomato seedlings (Solanum lycopersicum L.) by Trichoderma viride through the antioxidative defense system
Authors: Metwally Rabab A , Soliman Shereen A 
Published: February 2022

Abstract
Background: Species of Trichoderma are widely recognized for their biocontrol abilities, but little information regarding their mechanisms in promoting plant growth and enhancing its tolerance to salt stress are available. Salt stress is one of the main abiotic stresses restricting crop growth and productivity. Hence, this study aimed to investigate the NaCl effects on Trichoderma viride growth as well as on the seedlings morphological, physiological, and biochemical parameters of tomato (Solanum lycopersicum L.). Besides, the role of T. viride in promoting tomato seedling stress tolerance was scrutinized. Results: Results showed that 100 mM NaCl decreased the colony diameter of T. viride by 13.4 % compared to the control. Exposure of tomato seedlings to salt stress resulted in an overall decrease in growth, relative water content (RWC), and protein contents. At the same time, increases were found in proline, H2O2 content, malondialdehyde (MDA), as well as the activities of peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO), and ascorbate peroxidase (APX). Even though, with T. viride application, the salt negative effects were mitigated to a greater extent. Moreover, T. viride increased proline and total antioxidant capacity (TAC) in tomato seedlings at 100 mM NaCl by an average of 20.66 and 43.82 % compared to their comparable control. T. viride increased the activities of CAT, PPO, and APX enzymes by 74.6, 58.48, and 61.61% at 50 mM NaCl compared to non-saline control seedlings. As well, T. viride decreased MDA and H2O2 contents by an average of 14 and 24.8 % in tomato seedlings at 50 mM NaCl compared to their comparable control. Conclusion: Hence, our study provides new insight into the mechanisms of T. viride that can activate both enzymatic and non-enzymatic antioxidant defense systems and enhance tomato seedling tolerance to salt stress at morphological and physiological levels.
Bacterial and Archaeal Communities Within the Alkaline Na2CO3-type Langaco Lake in the Qinghai-Tibet Plateau
Authors: Wang Mingxiang , Zhang Xin , Shu Zhiwan , Wang Zhibo , Tao Yujie , Lv Chuanbo , Zhu Derui , Shen Guoping 
Published: February 2022

Abstract
Langaco Lake (LGL) is a strong Na2CO3-type lake that sits at an altitude of 4,548 m in the Qinghai-Tibet Plateau of China. LGL exhibits unique hydrochemical characteristics among Na2CO3-type lakes, but little is known about the microbial diversity of LGL and their interactions with environmental factors. Here, high-throughput sequencing of 16S rRNA genes revealed that LGL bacterial diversity comprised 327 genera in 24 phyla (4,871 operational taxonomic units [OTUs]; Shannon index values of 5.20–6.07) that represented significantly higher diversity than that of Archaea (eight phyla and 29 genera comprising 1,008 OTUs; Shannon index values of 2.98–3.30). The bacterial communities were dominated by Proteobacteria (42.79–53.70% relative abundances), followed by Bacteroidetes (11.13–15.18%), Planctomycetes (4.20–12.82%), Acidobacteria (5.91–9.50%), Actinobacteria (2.60–5.80%), and Verrucomicrobia (2.11–4.08%). Further, archaeal communities were dominated by Crenarchaeota (35.97–58.29%), Euryarchaeota (33.02–39.89%), and Woesearchaeota (6.50–21.57%). The dominant bacterial genus was Thiobacillus (8.92–16.78%), whose abundances were most correlated with total phosphorus (TP), pH, CO32− concentrations, and temperature. The most abundant archaeal genus was Methanoregula (21.40–28.29%), whose abundances were most highly correlated with TOC and TS in addition to the concentrations of K+, and Na+. Taken together, these results provide valuable insights towards a more comprehensive understanding of microbial diversity in these unique carbonate alkaline environments, in addition to a better understanding of microbial resources in the Qinghai-Tibet Plateau.
Design and Research of Digital Twin Machine Tool Simulation and Monitoring System
Authors: Guo Mingyi , Fang Xifeng , Hu Zhongtai , Li Qun 
Published: February 2022

Abstract
With the application of digital manufacturing technology and intelligent manufacturing technology in manufacturing industry, the existing simulation and monitoring methods of machine tools can not meet the requirements of enterprises, and gradually tend to use digital twin, OPC and other technologies to remote simulation and monitoring of machine tools. In order to prevent the accidental collision of machine tools, this paper builds the virtual model of machine tools based on the digital twin (DT) technology, detects the tool and machine tool collision model based on the improved GJK collision detection algorithm, and automatically controls the machine tool to stop when the tool and machine tool are about to collide. In order to improve the level of machine tool intelligent management, potential information extraction machine run time, this paper proposes a fast simulation method, machine tool CNC code used to detect the code is correct, and in the process of running through a visual component implementation monitoring the running state of the machine tool manufacturing process by monitoring data visualization and testing of large data, extracting the potential information, Realize the online prediction function of tool wear. Taking a turning process of Marine diesel engine cylinder liner as an example, the experimental test results show that the system can quickly judge the correctness of CNC code, effectively monitor the data of machine tool, predict tool wear degree and reduce the risk of tool collision.
Interactions of Environmental Pollutant Aromatic Amines with photoexcited states of Thiophene Substituted 1,3,4-Oxadiazole Derivative: Fluorescence quenching studies
Authors: Malimath G.H , M.S Thippeswamya , Naik Lohit , Maridevarmath C.V 
Published: February 2022

Abstract
In the present work, the ଂuorescence quenching of novel thiophene substituted 1,3,4-oxadiazole derivative 2-(4-(4-vinylphenyl) phenyl)-5-(5-(4-vinylphenyl)thiophen-2-yl)-1,3,4-oxadiazole (TSO) by five different environmental pollutant aromatic amine derivatives like 2,4-dimethylaniline, 3-chloroaniline, 4-chloroaniline, o-anisidine, and m-toluidine has been studied at room temperature through steady-state, and time-resolved methods. The fluorescence intensity of fluorophore TSO decreased as the concentration of aromatic amines increased. The fluorescence quenching mechanism between TSO and aromatic amines is analysed through Stern-Volmer plots. The steady state Stern-Volmer plots were found to be nonlinear with a positive deviation, whereas time resolved Stern-Volmer plots show linearity with an increase in quencher concentration. Further, the Stern-Volmer constants (k sv ), quenching rate parameters (k q ), static quenching constant (V) and radius of the sphere of action (r) are calculated. The magnitude of these parameters suggests that the sphere of action static quenching model is responsible for the overall quenching mechanism. Further, with the use of ଁnite sink approximation model to check these bimolecular reactions as diffusion-limited and to estimate independently S-V constant, the distance parameter (R'), mutual diffusion coefficient (D), and diffusion controlled parameter (k d ) were calculated. These values indicate that the bimolecular quenching reactions are due to the collective effect of dynamic and static quenching mechanisms and these results are confirmed by the time-resolved measurements. The higher values of Gibb’s free energy determined from the Rehm-Weller relation through cyclic voltammetry studies suggests that, the ଂuorescence quenching is due to electron transfer and the same is confirmed by the DFT theoretical studies. Further, the binding equilibria analysis confirm, 1:1 stoichiometry ratio between fluorophore and quencher and also strong binding between the fluorophore and aromatic amines.
Comparisons of Statistical Downscaling Methods for Air Temperature Over the Qilian Mountains
Authors: Jin Haodong , Li Xiaodong , Frauenfeld Oliver W. , Zhao Yaohua , Chen Cong , Du Ran , Du Jun , Peng Xiaoqing 
Published: February 2022

Abstract
Air temperature is an important indicator of climate change, as well as for understanding changes in hydrology, ecology, and other natural systems. However, meteorological stations that provide reliable temperature observations are usually sparse in areas of complex terrain, thus limiting our ability to quantify high spatial resolution temperature variability in these regions. Here, we apply three statistical downscaling methods to daily air temperature output from the sixth Coupled Model Intercomparison Project (CMIP6), validated with 22 meteorological stations over the Qilian Mountains. Based on different downscaling methods, we find RMSE and MAE are reduced as much as 59–66%, with the ratio of RMSE and MAE to the annual average temperature of stations decreasing from 147.9% to 61.0% and 143.3% to 64.7%, respectively, depending on the method. Compared to the original data, annual temperature based on the best downscaling methods differed by −2.85±3.61°C during the historical 1850–2014 period and, for the 2015–2100 projections, by 2.13±3.30°C for SSP1-2.6, −2.13±3.29°C for SSP2-4.5, −2.11±3.24°C for SSP3-7.0, and −2.12±3.23°C for SSP5-8.5. The downscaled annual air temperatures show a warming trend ranging 0.15­0.22°C/10yr for the historical experiment, 0.08°C­0.14°C/10yr for SSP1-2.6, 0.24­0.35°C/10yr for SSP2-4.5, 0.43­0.63°C/10yr for SSP3-7.0, and 0.52­0.76°C/10yr for SSP5-8.5 in the Qilian Mountain. These results indicate that the accuracy of the downscaled temperatures is improved compared to the original data. However, we also find that, compared with the downscaled data, the original projections have been overestimated.
Physiological and Transcriptome Analyses Reveal the Response of Ammopiptanthus Mongolicus to Extreme Seasonal Temperature in Plateau Cold Desert Ecosystem
Authors: Yang Zimeng , Liu Yiying , Han Hang , Zhao Xinyu , Chen Siyu , Li Guofang , Shi Sha , Feng Jinchao 
Published: February 2022

Abstract
Ammopiptanthus mongolicus is an evergreen broad-leaved shrub living in plateau cold desert ecosystem, which with remarkable tolerance to diverse abiotic stresses. We measured the photosynthetic physiological indexes of A. mongolicus. under extreme seasonal climate in the field, and analyzed the transcriptome of its leaves. We found that the photosynthetic physiological indexes and gene expression at transcriptome level have significant characteristics when temperature changes. Under the low temperature condition, A. mongolicus. consumes excess light energy by increasing PSII non-regulatory energy dissipation Y (NO) at the physiological level. At the molecular level, it resists low temperature stress by changing photosynthesis, circadian rhythm and synthesis of unsaturated fatty acids, but its photosynthetic machinery is still damaged and photosynthesis is blocked. At the physiological level, A. mongolicus. regulates its own water content and improves the regulation of the PSII regulatory energy dissipation Y(NPQ) to consumes excess light energy and to adapt to high temperature stress. At the molecular level, A. mongolicus. protects itself from high temperature and strong light by using the LHCII of PSII to consume excess energy, continuously assembling and maintaining PSII, and changing the production of antioxidants and so on.
Identiﬁcation of Pyroptosis-related Gene Prognostic Signature in Patients with Hepatocellular Carcinoma
Authors: Chen Ruipeng , Li Pansong , Song Mengmeng , Ji Liyan , Sun Yi , Gao Xuan , Xia Xuefeng 
Published: February 2022

Abstract
Background: Pyroptosis has been recently identified as a hallmark of cancer biology; however, the potential of pyroptosis-related genes (PRGs) as prognostic markers has not been fully elucidated in hepatocellular carcinoma (HCC). Methods:35 PRGs were obtained from the published literature, and pyroptosis subtypes were identified by bioinformatics methods. The risk score model was established applying the LASSO Cox regression method in the TCGA cohort and validated in the ICGC datasets. Additionally, immune infiltration, enriched pathways and genomic alterations were analyzed between high and low risk-score subgroups. Finally, a nomogram containing the pyroptosis risk score and other prognostic related clinical factors was created for predicting the overall survival of HCC.Results:Based on the expression profile of PRGs, we determined two pyroptosis-related subtypes (cluster A and cluster B) of HCC with different immune characteristics and significantly different prognoses. The risk score model showed that up-regulation of GPX4, CASP8, NOD2, and GSDME was associated with poor prognosis, while NLRP6 was the opposite. Compared with patients with lower risk scores, the high-risk score group had a worse prognosis (P <0.0001) and a higher level of immune cell infiltration. Functional analysis suggested that the highly expressed genes in the high-risk group were mainly concentrated in various signaling pathways, while the low-expressed genes in the high-risk group were mainly enriched in different biochemical metabolic translations. Genomic alterations in high-risk and low-risk populations implied that mutations in the TP53 gene are highly associated with pyroptosis in HCC patients. A nomogram including risk score and TNM stage for 1-year, 3-year, and 5-year overall survival indicated a good prognostic prediction ability.Conclusions:We developed and verified a prognostic risk model based on PRGs for HCC sufferers, which may provide a robust tool for forecasting prognosis in patients with HCC.
Efficient nitrogen and phosphorus removal from wastewater by screening and constructing a novel Chlorella sp.-Bacillus taeanensis SD148BN12 consortium
Authors: Liu Liping , Zhang Mengyu , Li yun , Pan Luqing , Dou Le , Su Zhaopeng 
Published: February 2022

Abstract
The discharge of wastewater rich in nitrogen and phosphorus were posing a serious threat to ecological balance and human health. In the study, Bacillus taeanensis SD148BN12 with biosafety and capability of aerobic nitrogen removal by assimilation was selected to construct the Chlorella sp.-Bacillus taeanensis SD148BN12 consortium (M-B). Compared with single axenic Chlorella sp. and single Bacillus taeanensis SD148BN12, M-B improved the removal efficiencies of total nitrogen (91.89%) and total phosphorus (94.58%), the biomass yield (0.52 g/L), as well as the contents of carbohydrate and lipid after cultivation in synthetic wastewater. M-B had the high and stable nitrogen removal efficiency at temperature 20-30 ℃, salinity 20-40‰, light intensity 50-200 μmol photons m-2 s-1, pH 6-9, C/N ratio 1-10, N/P ratio 4-20, N concentration 1-50 mg/L. The conspicuous removal efficiency of nitrogen and phosphorus from actual marine wastewater could be realized by M-B. Meanwhile, the synergistic interaction between Chlorella sp. and strain SD148BN12 was demonstrated by the characteristics of cell growth, photosynthetic activity and material exchange. The study provided an economical and environmentally friendly strategy for efficient removal of nitrogen and phosphorus from wastewater (including the neglected aquaculture wastewater) by establishing a hopeful microalgae-bacteria consortium.
Elucidation of Melanin Pigment Production From A Promising Locally Isolated Halotolerant Black Yeast Hortaea Werneckii AS1
Authors: Elsayis Asmaa , Hassan Sahar W. M. , Ghanem Khaled M. , Khairy Heba 
Published: February 2022

Abstract
Background: Melanins are one of the magnificent natural pigments synthesized by a wide range of microorganisms including different species of fungi and bacteria. Marine black yeasts appear to be potential prospects for the synthesis of natural melanin pigment production. As a result, the goal of this research was to isolate a promising marine black yeast melanin-producing strain and improve the culturing conditions in order to maximize the yield of such a valuable pigment.Results: Among six locally isolated black yeast strains, the only one that demonstrated a potent remarkable melanin pigment production was Identified using 18s rRNA as Hortaea werneckii AS1. The extracted pigment’s physiochemical characterization and analytical investigation with Ultraviolet-Visible (UV) spectrophotometry, Fourier Transform-Infrared spectroscopy (FTIR), and Scanning Electron Microscope (SEM) confirmed its nature as a melanin pigment. The data obtained from the polynomial model’s maximum point suggested that CaCl2, 1.125 (g/L); trace element, 0.25 (ml/L); and a culture volume 225 (ml/500ml) at their optimal values were the critical three elements impacting melanin production. In comparison with the baseline settings, the response surface methodology (RSM) optimization approach resulted in a 2.0 - fold improvement in melanin output. Conclusions: A Maximum melanin yield of 0.938 g/L proved the halotolerant H. werneckii AS1 potentiality as a source for natural melanin pigment synthesis ‘when compared to some relevant black yeast strains’ and hence, facilitating its incorporation in a variety of pharmaceutical and environmental applications.
Application of Nanomagnetic NH 2 .MIL-101(Fe)/ED as a Retrievable and Efficient Catalyst for the Synthesis of 2,6-Diamino-4-Aryl-4H-Thiopyran- 3,5- Dicarbonitrile Derivatives
Authors: Moghaddam Firouz Matloubi , Aghamiri Bagher , Motlagh Aida Yazdani , Jarahiyan Atefeh 
Published: February 2022

Abstract
In this publication, we reported an efficient one-pot, four-component procedure for the synthesis of 2,6-diamino-4-Aryl-4H-Thiopyran-3,5-dicarbonitrile derivatives by a reaction between primary amines, carbon disulfide, malononitrile, and benzylidenemalononitrile in the presence of magnetic NH2.MIL-101(Fe)/ED as a basic metal organic framework catalyst. Magnetic NH2.MIL-101(Fe)/ED was synthesized through anchoring FeCl3 on CoFe2O4 magnetic nanoparticles surface and then 2-aminoterphthalic acid was used to form MOF structure. At the final step, metal centers were modified with ethylenediamine (ED). The different techniques such as Fourier transmission infrared spectroscopy, X-ray diffraction, Field emission scanning electron microscopy, Transmission electron microscopy, Brunauer-Emmett-Teller analysis, and Thermogravimetric analysis were used to characterize the structure of the catalyst. Using X-ray crystallography, the structure of the final product was also determined.
Genome Wide Association Mapping of Yield And Various Desirable Agronomic Traits In Rice
Authors: Ashfaq Muhammad , Rasheed Abdul , Sajjad Muhammad , Ali Muhammad , Rasool Bilal , Javed Muhammad Arshad , Shaheen Shabnum , Anwar Alia , Allah Sami Ul , Ahmad Muhammad Shafiq , Mubashar Urooj 
Published: February 2022

Abstract
Background: Rice (Oryza sativa L.) is one of the staple foods worldwide. To feed the growing population, improvement of rice cultivar is important. To make the improvement in rice breeding program, it is imperative to understand the similarity of the existing rice accessions. Previous studies demonstrated the existence of abundant elite genes in rice landraces. A genome-wide association study (GWAS) was performed for yield and yield related traits to find the elite genes. Design: Experimental studyMethods and results: A total of 204 SSRs markers were used among 17 SSRs were found to be located on each chromosome in the rice genome. The diversity was analyzed using different genetic characters i.e., the total number of alleles (TNA), polymorphic information content (PIC) and gene diversity by Pwer markers and the values for each genetic character per marker ranged from 2 to 9, 0.332 to 0.887 and 0.423 to 0.900 respectively across the whole genome. The results of population structure identified four main groups. MTA identified several markers associated with many agronomically important traits. These results will be very useful for the selection of potential parents, recombinants and MTAs that governing the improvements and developments of new high yielding rice varieties. Conclusion: Analysis of diversity in germplasm is important for improvement of cultivars in breeding program. In the present study, the diversity was analyzed with different methods and found that enormous diversity was present in studied rice germplasm. The structure analysis found the presence of 4 genetic groups in the existing germplasm. A total of 129 marker-trait associations (MTAs) have been found in this study.