bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED ARTICLE: Strontium doped bioglass
incorporated hydrogel-based scaffold for amplified bone tissue regeneration
Authors: Manoochehri Hamed , Ghorbani Masoud , Moosazadeh Moghaddam Mehrdad , Nourani Mohammad Reza , Makvandi Pooyan , Sharifi Esmaeel 
Published: June 2022

AbstractRepairing of large bone injuries is an important problem in bone
regeneration field. Thus, developing new therapeutic approaches such as tissue
engineering using 3D scaffolds is necessary. Incorporation of some bioactive materials
and trace elements can improve scaffold properties. We made
chitosan/alginate/strontium-doped bioglass composite scaffolds with optimized properties
for bone tissue engineering. Bioglass (BG) and Sr-doped bioglasses (Sr-BG) were
synthesized using Sol–Gel method. Alginate-Chitosan (Alg/Cs) scaffold and scaffolds
containing different ratio (10%, 20% and 30%) of BG (Alg/Cs/BG10, 20, 30) or Sr-BG
(Alg/Cs/Sr-BG10, 20, 30) were fabricated using freeze drying method. Characterization of
bioglasses/scaffolds was done using zeta sizer, FTIR, XRD, (FE) SEM and EDS. Also,
mechanical strength, antibacterial effect degradation and swelling profile of scaffolds
were evaluated. Bone differentiation efficiency and viability of MSCs on scaffolds were
determined by Alizarin Red, ALP and MTT methods. Cell toxicity and antibacterial effect
of bioglasses were determined using MTT, MIC and MBC methods. Incorporation of BG into
Alg/Cs scaffolds amplified biomineralization and mechanical properties along with
improved swelling ratio, degradation profile and cell differentiation. Mechanical
strength and cell differentiation efficiency of Alg/Cs/BG20 scaffold was considerably
higher than scaffolds with lower or higher BG concentrations. Alg/Cs/Sr-BG scaffolds had
higher mechanical stability and more differentiation efficiency in comparison with
Alg/Cs and Alg/Cs/BG scaffolds. Also, Mechanical strength and cell differentiation
efficiency of Alg/Cs/Sr-BG20 scaffold was considerably higher than scaffolds with
various Sr-BG concentrations. Biomineralization of Alg/Cs/BG scaffolds slightly was
higher than Alg/Cs/Sr-BG scaffolds. Overall, we concluded that Alg/Cs/Sr-BG20 scaffolds
are more suitable for repairing bone major injuries.
Association of weekend catch-up sleep ratio and subjective sleep quality with depressive symptoms and suicidal ideation among Korean adolescents
Authors: Lee Hyunseo , Kim Yeon-Jeong , Jeon Yong-Hyun , Kim Seung Hoon , Park Eun-Cheol 
Published: June 2022

AbstractCircadian misalignment caused by differences in sleep duration between weekends and weekdays may be associated with adolescent mental health and sleep quality may be able to compensate for this problem. This study aimed to investigate the association between weekend catch-up sleep (CUS) ratio and sleep quality with depressive symptoms and suicidal ideation among South Korean adolescents. We used data from the Korea Youth Risk Behavior Web-based Survey 2015–2019 involving 270,619 adolescents. The weekend CUS ratio was calculated by dividing the average weekend sleep duration by the average weekday sleep duration (< 1.00, 1.00 ≤ CUS < 1.50, or ≥ 1.50). Subjective sleep quality was categorized as poor, moderate, or good. Multiple logistic regression analyses were performed. A weekend CUS ratio of < 1.00 and poor sleep quality was significantly associated with mental health. Absolutely short sleep duration (CUS < 1.00 and weekday sleep duration < 5 h) was most associated with depressive symptoms and suicidal ideation. Furthermore, adolescents with a CUS ratio of ≥ 1.50 showed increased odds of depressive symptoms despite having good sleep quality. Appropriate weekend CUS may benefit adolescents’ mental health. When investigating the relationship between adolescents’ sleep and mental health, a weekend CUS ratio should be considered in addition to sleep quality and duration.
Shared and distinct voxel-based lesion-symptom mappings for spasticity and impaired movement in the hemiparetic upper limb
Authors: Frenkel-Toledo Silvi , Levin Mindy F. , Berman Sigal , Liebermann Dario G. , Baniña Melanie C. , Solomon John M. , Ofir-Geva Shay , Soroker Nachum 
Published: June 2022

AbstractHemiparesis and spasticity are common co-occurring manifestations of hemispheric stroke. The relationship between impaired precision and force in voluntary movement (hemiparesis) and the increment in muscle tone that stems from dysregulated activity of the stretch reflex (spasticity) is far from clear. Here we aimed to elucidate whether variation in lesion topography affects hemiparesis and spasticity in a similar or dis-similar manner. Voxel-based lesion-symptom mapping (VLSM) was used to assess the impact of lesion topography on (a) upper limb paresis, as reflected by theFugl-Meyer Assessmentscale for the upper limb and (b) elbow flexor spasticity, as reflected by theTonic Stretch Reflex Threshold, in 41 patients with first-ever stroke. Hemiparesis and spasticity were affected by damage to peri-Sylvian cortical and subcortical regions and the putamen. Hemiparesis (but not spasticity) was affected by damage to the corticospinal tract at corona-radiata and capsular levels, and by damage to white-matter association tracts and additional regions in the temporal cortex and pallidum. VLSM conjunction analysis showed only a minor overlap of brain voxels where the existence of damage affected both hemiparesis and spasticity, suggesting that control of voluntary movement and regulation of muscle tone at rest involve largely separate parts of the motor network.
Universal association between depressive symptoms and social-network structures in the workplace
Authors: Lee Jong-Hyeok , Sato Nobuo , Yano Kazuo , Miyake Yoshihiro 
Published: June 2022

AbstractAn unhealthy communication structure at a workplace can adversely affect the mental health of employees. However, little is known about the relationship between communication structures in the workplace and the mental health of employees. Here, we evaluated the face-to-face interaction network among employees (N = 449) in a variety of real-world working environments by using wearable devices and investigated the relationship between social network characteristics and depressive symptoms. We found that the cohesive interaction structure surrounding each individual was negatively correlated with depressive symptoms: a universal relationship regardless of occupation type. This correlation was evident at the group scale and was strongly related to active interactions with abundant body movement. Our findings provide a quantitative and collective perspective on taking a systematic approach to workplace depression, and they suggest that the mental health of employees needs to be addressed systematically, not only individually.
Real-world treatment intensities and pathways of macular edema following retinal vein occlusion in Korea from Common Data Model in ophthalmology
Authors: Mun Yongseok , Park ChulHyoung , Lee Da Yun , Kim Tong Min , Jin Ki Won , Kim Seok , Chung Yoo-Ri , Lee Kihwang , Song Ji Hun , Roh Young-Jung , Jee Donghyun , Kwon Jin-Woo , Woo Se Joon , Park Kyu Hyung , Park Rae Woong , Yoo Sooyoung , Chang Dong-Jin , Park Sang Jun 
Published: June 2022

AbstractDespite many studies, optimal treatment sequences or intervals are still questionable in retinal vein occlusion (RVO) macular edema. The aim of this study was to examine the real-world treatment patterns of RVO macular edema. A retrospective analysis of the Observational Medical Outcomes Partnership Common Data Model, a distributed research network, of four large tertiary referral centers (n = 9,202,032) identified 3286 eligible. We visualized treatment pathways (prescription volume and treatment sequence) with sunburst and Sankey diagrams. We calculated the average number of intravitreal injections per patient in the first and second years to evaluate the treatment intensities. Bevacizumab was the most popular first-line drug (80.9%), followed by triamcinolone (15.1%) and dexamethasone (2.28%). Triamcinolone was the most popular drug (8.88%), followed by dexamethasone (6.08%) in patients who began treatment with anti-vascular endothelial growth factor (VEGF) agents. The average number of all intravitreal injections per person decreased in the second year compared with the first year. The average number of injections per person in the first year increased throughout the study. Bevacizumab was the most popular first-line drug and steroids were considered the most common as second-line drugs in patients first treated with anti-VEGF agents. Intensive treatment patterns may cause an increase in intravitreal injections.
In vitro and in vivo antimalarial activity and chemical profiling of sugarcane leaves
Authors: Okokon Jude E. , Mobley Rebecca , Edem Utibe A. , Bassey Augustine I. , Fadayomi Idowu , Drijfhout Falko , Horrocks Paul , Li Wen-Wu 
Published: June 2022

AbstractSaccharum officinarumLinn. (sugarcane, Family-Poaceae) is employed in Ibibio traditional medicine for the treatment of various infections and diseases such as malaria. We This study aims to assess the antiplasmodial effect of the leaf extract and fractions on human malaria parasite (Plasmodium falciparum) in vitro, and rodent malaria parasite (P. berghei) in vivo, and analyse the bioactive components of the active fraction(s). The leaf extract and fractions ofS. officinarumwere prepared and their growth inhibitory effects tested against the chloroquine resistantP. falciparumstrain (Dd2) andP. bergheiinfection in mice. An acute toxicity of the extract was determined. A combination of gas chromatography and liquid chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy was applied for metabolites profiling of crude extract and active fractions. The leaf extract and fractions demonstrated moderate activity againstP. falciparumwith the dichloromethane fraction producing the most potent activity (EC50 = 15.4 µg/mL). The leaf extract (170–510 mg/kg, p.o., LD50 = 1732 mg/kg) and fractions demonstrated significant (p < 0.05–0.001) effect onP. bergheiinfection in prophylactic tests as well as in established infection withn-butanol fractions producing the highest effect. An unusual sulphur-containing compound, dilaurylthiodipropionate, fatty acids, phenolic acids, flavonoid and flavonoid glycoside were identified in the active fractions. These results give credence to the use of sugarcane leaves as malarial remedy locally by confirming the in vitro and in vivo antiplasmodial potential of leaf extract/fractions ofS. officinarum.
Epidemiological characterization of SARS-CoV-2 variants in children over the four COVID-19 waves and correlation with clinical presentation
Authors: Alteri Claudia , Scutari Rossana , Costabile Valentino , Colagrossi Luna , Yu La Rosa Katia , Agolini Emanuele , Lanari Valentina , Chiurchiù Sara , Romani Lorenza , Markowich Anna Hermine , Bernaschi Paola , Russo Cristina , Novelli Antonio , Bernardi Stefania , Campana Andrea , Villani Alberto , Perno Carlo Federico 
Published: June 2022

AbstractSince the start of SARS-CoV-2 pandemic, children aged ≤ 12 years have always been defined as underrepresented in terms of SARS-CoV-2 infections’ frequency and severity. By correlating SARS-CoV-2 transmission dynamics with clinical and virological features in 612 SARS-CoV-2 positive patients aged ≤ 12 years, we demonstrated a sizeable circulation of different SARS-CoV-2 lineages over the four pandemic waves in paediatric population, sustained by local transmission chains. Age < 5 years, highest viral load, gamma and delta clades positively influence this local transmission. No correlations between COVID-19 manifestations and lineages or transmission chains are seen, except for a negative correlation between B.1.1.7 and hospitalization.
Clinical characteristics of external bacterial ocular and periocular infections and their antimicrobial treatment patterns among a Ghanaian ophthalmic population
Authors: Osei Duah Junior Isaiah , Tchiakpe Michel Pascal , Borquaye Lawrence Sheringham , Amoah Kwadwo , Amankwah Francis Kwaku Dzideh , Kumah David Ben , Ofori Linda Aurelia , Danso-Appiah Anthony , Prempeh Bright Owusu , Gbedema Stephen Yao , Munyaneza Justin , Danquah Cynthia Amaning , Akuffo Kwadwo Owusu 
Published: June 2022

AbstractEmpirical antimicrobial therapy is linked to a surge in antimicrobial resistant infections. However, an insight on the bacteria etiology of ocular infections is essential in the appropriation of choice of antimicrobial among clinicians, yet there remains a dearth of data from Ghana. We investigated the bacteria etiology of external ocular and periocular infections and antimicrobial treatment patterns among a Ghanaian ophthalmic population. A multicenter study design with purposive sampling approach was employed. Patients demographics and clinical data were collated using a pretested structure questionnaire. Cornea specimens and conjunctival swabs were obtained for bacterial isolation following standard protocols. About 95% (98/103) of ocular samples were positive for bacteria culture. The proportion of Gram-negative bacteria was 58.2%, and the predominant bacteria species were Pseudomonas aeruginosa 38.8% and Staphylococcus aureus 27.6%. Conjunctivitis 40.0% and keratitis 75.0% were mostly caused by Pseudomonas aeruginosa. The routinely administered antimicrobial therapy were polymyxin B 41.2%, neomycin 35.1% and ciprofloxacin 31.6%. Participants demographic and clinical characteristics were unrelated with positive bacteria culture (p > 0.05). Our results showed a markedly high burden of ocular bacterial infections and variations in etiology. Bacterial infection-control and antimicrobial agent management programs should be urgently institutionalized to prevent the emergence of resistant infections.
Streamlined and quantitative detection of chimerism using digital PCR
Authors: Suchy Fabian P. , Nishimura Toshiya , Seki Shinsuke , Wilkinson Adam C. , Higuchi Maimi , Hsu Ian , Zhang Jinyu , Bhadury Joydeep , Nakauchi Hiromitsu 
Published: June 2022

AbstractAnimal chimeras are widely used for biomedical discoveries, from developmental biology to cancer research. However, the accurate quantitation of mixed cell types in chimeric and mosaic tissues is complicated by sample preparation bias, transgenic silencing, phenotypic similarity, and low-throughput analytical pipelines. Here, we have developed and characterized a droplet digital PCR single-nucleotide discrimination assay to detect chimerism among common albino and non-albino mouse strains. In addition, we validated that this assay is compatible with crude lysate from all solid organs, drastically streamlining sample preparation. This chimerism detection assay has many additional advantages over existing methods including its robust nature, minimal technical bias, and ability to report the total number of cells in a prepared sample. Moreover, the concepts discussed here are readily adapted to other genomic loci to accurately measure mixed cell populations in any tissue.
Mmp13 deletion in mesenchymal cells increases bone mass and may attenuate the cortical bone loss caused by estrogen deficiency
Authors: Ponte Filipa , Kim Ha-Neui , Warren Aaron , Iyer Srividhya , Han Li , Mannen Erin , Gomez-Acevedo Horacio , Nookaew Intawat , Almeida Maria , Manolagas Stavros C. 
Published: June 2022

AbstractThe protective effect of estrogens against cortical bone loss is mediated via direct actions on mesenchymal cells, but functional evidence for the mediators of these effects has only recently begun to emerge. We report that the matrix metalloproteinase 13 (MMP13) is the highest up-regulated gene in mesenchymal cells from mice lacking the estrogen receptor alpha (ERα). In sham-operated female mice with conditional Mmp13 deletion in Prrx1 expressing cells (Mmp13ΔPrrx1), the femur and tibia length was lower as compared to control littermates (Mmp13f./f). Additionally, in the sham-operated female Mmp13ΔPrrx1 mice cortical thickness and trabecular bone volume in the femur and tibia were higher and osteoclast number at the endocortical surfaces was lower, whereas bone formation rate was unaffected. Notably, the decrease of cortical thickness caused by ovariectomy (OVX) in the femur and tibia of Mmp13f./f mice was attenuated in the Mmp13ΔPrrx1 mice; but the decrease of trabecular bone caused by OVX was not affected. These results reveal that mesenchymal cell–derived MMP13 may regulate osteoclast number and/or activity, bone resorption, and bone mass. And increased production of mesenchymal cell-derived factors may be important mediators of the adverse effect of estrogen deficiency on cortical, but not trabecular, bone.
Death anxiety as mediator of relationship between renunciation of desire and mental health as predicted by Nonself Theory
Authors: Kuo Yi-Fen , Chang Yun-Ming , Lin Mei-Fang , Wu Ming-Lung , Shiah Yung-Jong 
Published: June 2022

AbstractIn the present paper, we report two studies examining the relationships among renunciation of desires, death anxiety, and mental health. In the first study, we constructed the Desire Questionnaire (DQ), which measures success in renouncing certain desire. The DQ has satisfactory psychometric properties. In the second study, 501 adults from a Chinese society ranging in age from 17 to 84 years (M = 35.58, SD = 14.76) completed the DQ, the Death Anxiety Scale (DAS), and the Chinese Health Questionnaire (CHQ), which measures mental health and the presence of psychiatric symptoms. As predicted, DQ had significant negative correlations with both DAS (p < 0.05) and CHQ (p < 0.01). DAS had a significant positive correlation with CHQ (p < 0.01). In the linear mediation model, DAS was found to partially mediate the relationship between DQ and CHQ (β = − 0.18, p < 0.001). These results suggest that the negative effect of poor control of desires on mental health can be partially accounted for by death anxiety. These findings imply that training in eliminating desires can be a successful strategy to improve mental health. These results also support the Nonself Theory as a theory of death anxiety and show its relevance to the relationship between nonself and mental health.
Irreversibility analysis for flow of nanofluids with aggregation in converging and diverging channel
Authors: Qadeer Muhammad , Khan Umar , Ahmad Sarfraz , Ullah Basharat , Mousa Mohamed , Khan Ilyas 
Published: June 2022

AbstractIn the current research article, the two-dimensional, incompressible, steady fluid flow is considered. The heat transfer rate of water-based aggregated fluid between converging/diverging channels of shrinking/stretching walls due to the effects of thermal radiation has been examined. The strong static magnetic field is applied perpendicular to the radial direction. The modeled governing equations are transformed into non-linear dimensionless ordinary differential equations by considering appropriate similarity transformations. Since the obtained ODEs are strongly non-linear and the exact solution of these equations is not possible, thus we applied the numerical method RK4 combined with the shooting technique to handle the equations. The impacts of several influential parameters on velocity, temperature, and entropy generation profiles are examined graphically.
Horticulture therapy affected the mental status, sleep quality, and salivary markers of mucosal immunity in an elderly population
Authors: Shen Jui-Ling , Hung Bao-Lien , Fang Shih-Hua 
Published: June 2022

AbstractHorticultural therapy (HT) has been reported to be beneficial to mental and physical health. This study investigated the effects of HT on the psychological status and mucosal immunity of elderly individuals. Twenty-four participants aged 70–93 were recruited from residential facilities and adult day-care services. Six different HT activities were designed and guided by licensed instructors who performed saliva collection and helped the participants complete the questionnaires before and after each activity. The sleep quality scores were collected during the 6 weeks of HT activities. Saliva was collected and analyzed to determine the concentrations of immunoglobulin A (IgA), lactoferrin, chromogranin A (CgA), α-amylase (AA) and total protein (TP). Comparisons of the questionnaire scores between preactivity and postactivity showed that feelings of satisfaction and happiness were significantly enhanced after each activity. In addition, sleep quality was significantly improved after the 6-week course of HT activities. Regarding mucosal immunity, the preactivity IgA and IgA/TP were significantly increased at week 3 and week 6; in addition, the ratio of lactoferrin/TP was significantly decreased at week 6 compared to week 1. The postactivity AA and CgA levels were significantly enhanced at weeks 2, 3 and 5 compared to the corresponding preactivity levels. In conclusions, HT activities significantly improved the happiness, satisfaction, well-being and sleep quality of the elderly. Moreover, mucosal immunity proteins, including IgA, lactoferrin, CgA and AA, were significantly increased.
Three-dimensional in situ imaging of single-grain growth in polycrystalline In2O3:Zr films
Authors: Dzhigaev Dmitry , Smirnov Yury , Repecaud Pierre-Alexis , Marçal Lucas Atila Bernardes , Fevola Giovanni , Sheyfer Dina , Jeangros Quentin , Cha Wonsuk , Harder Ross , Mikkelsen Anders , Wallentin Jesper , Morales-Masis Monica , Stuckelberger Michael Elias 
Published: June 2022

AbstractStrain and interactions at grain boundaries during solid-phase crystallization are known to play a significant role in the functional properties of polycrystalline materials. However, elucidating three-dimensional nanoscale grain morphology, kinetics, and strain under realistic conditions is challenging. Here, we image a single-grain growth during the amorphous-to-polycrystalline transition in technologically relevant transparent conductive oxide film of In2O3:Zr with in situ Bragg coherent X-ray diffraction imaging and transmission electron microscopy. We find that the Johnson-Mehl-Avrami-Kolmogorov theory, which describes the average kinetics of polycrystalline films growth, can be applied to the single grains as well. The quantitative analysis stems directly from imaging results. We elucidate the interface-controlled nature of the single-grain growth in thin films and reveal the surface strains which may be a driving force for anisotropic crystallization rates. Our results bring in situ imaging with coherent X-rays towards understanding and controlling the crystallization processes of transparent conductive oxides and other polycrystalline materials at the nanoscale.
Power imbalance‐based droop control for vehicle to grid in primary frequency regulation
Authors: Suh Jaewan , Song Sungyoon , Jang Gilsoo 
Published: June 2022

AbstractAs demand for electric vehicles (EV) grows, interest in vehicle‐to‐grid (V2G) is increasing. Although V2G provides additional flexibility for power systems, it can be an inconvenience for the EV owner and shorten the cycle life of EV batteries. In this paper, we propose a power‐imbalance‐based droop control (PIDC) for V2G, which uses estimated active power imbalance between supply and demand, without any communications or dispatch signal from the automatic generation control (AGC). Unlike the existing frequency droop control, the proposed method controls V2G based on the estimated imbalance. The Imbalance between electricity supply and demand was estimated using the swing equation, frequency deviation, and rate of change of frequency (RoCoF). Conventional frequency droop control has a slow response speed because it only considers the frequency deviation. Using the PIDC, the critical frequency drop was discerned faster and the V2G could react more quickly in critical frequency drop situations. Additionally, battery cycle life was preserved in ordinary frequency fluctuations where V2G is unnecessary. This study used MATLAB R2020a to show the effectiveness and performance of PIDC. The case study results showed that PIDC has a faster response speed and preserves EV battery cycle life compared to frequency droop control.