bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of Citalopram, Swimming, and Treadmill Exercises on Learning and Spatial Memory of Male Wistar Rats under Stress
Authors: Molamohammadi Akram , Shasaltaneh Marzieh Dehghan , Naghdi Nasser , Hemayattalab Rasool , Zandi Hassan Gharayagh 
Published: December 2022

Abstract
Purpose: Several factors such as stress disturb learning and memory, and interfere with normal human life. The present study aimed to compare the effects of citalopram, aerobic exercise with treadmill, and swimming on learning and memory among stressed rats.
Methods: The animals were exposed to different interventions such as immobility stress, running with gentle intensity, exercise in water, taking citalopram at a dose of 10 mg/kg body weight for four weeks, and a combination of these interventions with stress or without intervention.
Results: The results showed that stress affects learning and memory based on the distance traveled to the platform. The exercises counteracted stress-induced functional impairment, and swimming had a positive effect on learning and memory in stressed rats. However, contrary to expectations, taking citalopram did not prevent stress-induced dysfunction. In stress-free conditions, the interactive effects of treadmill and swimming training between sports groups (swimming and treadmill), control group, and stress groups were not significant. None of these interventions affected the recall. However, they were effective only in the acquisition phase.
Conclusions: Exercising especially swimming, could be a substitute medication intervention since the effect of exercise with treadmill and that of water significantly differed by using citalopram while placing in stressful situations.
Biodiversity and molecular characterization of Klebsiella pneumoniae isolated from some soil invertebrates at high altitudes and the potential activities of Rhazya stricta extracts.
Authors: Hassan Mohamed M. , Albogami Bander , Mwabvu Tarombera , Awad Mohamed F. , Kadi Roqayah H. , Mohamed Alaa , Hassan Montaser 
Published: December 2022

Abstract
Background
Klebsiella is a common pathogen that is dangerous to humans and other animals, including invertebrates, and is widely present in the digestive system. It also appears that the genus Klebsiella is ubiquitous, as it is endemic to surface water, soil, and sewage.
Methods
In this study, 70 samples were obtained from soil-dwelling invertebrates from September 2021 to March 2022 from Taif and Shafa in different altitudinal regions. Fifteen of these samples contained Klebsiella spp. The Klebsiella isolates were genetically identified as Klebsiella pneumoniae using rDNA sequencing. The antimicrobial susceptibility of the Klebsiella isolates was determined. Amplification of virulence genes was performed using PCR.
Results
In this study, 16S rDNA sequencing showed similarity from 98–100% with related K. pneumonia from the NCBI database, and the sequences were deposited in NCBI GenBank under accession numbers ON077036 to ON077050. We then screened the growth inhibition properties of ethanolic and methanolic extracts of the medicinal plant Rhazya stricta leaves against some K. pneumoniae strains using minimum inhibitory concentration (MIC) methods and disc diffusion. In addition, the biofilm inhibitory potential of these extracts was investigated using crystal violet. HPLC analysis identified 19 components divided into six flavonoids, 11 phenolic acids, stilbene (resveratrol), and quinol, and revealed variations in the number of components and their quantities between extracts. Both extracts demonstrated interesting antibacterial properties against the K. pneumoniae isolates. The two extracts also showed strong biofilm inhibitory activities, with percentages of inhibition extending from 81.5–98.7% and from 35.1–85.8% for the ethanolic and methanolic extracts, respectively.
Conclusion
Rhazya stricta leaves extract revealed powerful antibacterial and antibiofilm activities against K. pneumoniae isolates and could be good candidates for the treatment or prevention of K. pneumonia-related infections.
Telomere dynamics in healthy expert meditators: a case control study
Authors: Dasanayaka Nirodhi Namika , Sirisena Nirmala Dushyanthi , Samaranayake Nilakshi 
Published: December 2022

Abstract
Practice of meditation has been suggested to improve telomere regulation. This case-control study compared the leucocyte telomere length (TL) and expression of hTERT and hTR genes, between thirty long-term, skilled meditators and thirty matched non-meditators. 63.34% of the participants were males and the average age of the meditators was 43.83±9.92 years. Meditators had practiced meditation over 6.8 years (±3.27) with 5.82 hours (±3.45) of average daily meditation. TL was found to decrease significantly with increasing age (r=-0.675, p<0.001) but was not associated with other biological variables. The long-term meditators had a significantly longer relative TL compared to the control group (p=0.020). Expression of hTERT (FC=1.3, p=0.020) and hTR (FC=1.4, p=0.029) genes showed significant up-regulation in long-term meditators compared to controls. Further, telomere length (r=0.838, p<0.001) and expression of hTR (r=0.378, p=0.039) and hTERT (r=0.622, p<0.001) genes showed a significant increase with the increasing duration of meditation practice. The regression analysis linking previous data from the same cohort indicated that the expression of hTERT gene significantly predicted the plasma telomerase level (p=0.034). The findings suggest that meditation as a lifestyle practice has multi-level beneficial effects on telomere dynamics with potential to promote healthy aging.
The Antibacterial property of ZnO Nanopowder with different concentration of Sodium Hydroxide prepared by hydrothermal method
Authors: Taha Ahmed B. , Murad Hind I. , Mohammed Mohammed A. , Jamal Raied K. 
Published: December 2022

Abstract
Zinc oxide Nanopowder was synthesized through a hydrothermal method in this study. The effect of three different NaOH concentrations on the properties of ZnO Nanopowder was studied. The hexagonal wurtzite structure of all ZnO Nanopowder is confirmed by X-ray diffraction analysis, with average crystallite sizes in the range of (33.5–30.4) nm. It can be observed that the crystallite size become smaller with increasing NaOH concentration. Scanning electron microscopy analysis indicates that ZnO has hexagonal shape uniform particle size distribution and their morphology was grain-like. Atomic force microscope shows that the ZnO Nanopowder average surface roughness decreased from (9.33–5.06) nm due to increasing NaOH concentration. The Fourier transform infrared spectroscopy peaks indicate successful preparation of ZnO Nanopowder. From the optical absorption spectra the band gap energy has been calculated. The NaOH concentrations have been found very effective on the energy gap value. So, its increased from (3.27 to 3.36) eV when the NaOH concentration is increased. ZnO Nanopowder shows high antibacterial activity against P.aeuruginosa and S. aureus bacteria. The ZnO Nanopowder shows has very high antibacterial activity on gram-positive (S. aureus) compared with antibacterial activity on the gram-negative (P.aeuruginosa) bacteria.
The prognostic significance of Lung Immune Prognostic Index (LIPI) and Pan-immune-inflammation value (PIV) in patients with large cell neuroendocrine carcinoma
Authors: Menekse Serkan , Kut Engin 
Published: December 2022

AbstractLarge cell neuroendocrine carcinomas (LCNEC) are rare tumors of the lung. Due to their rarity, there is no standard follow-up and treatment. Inflammation and immune systems play an important role in the pathogenesis, development, and progression of lung and other cancers. Lung Immune Prognostic Index (LIPI) and Pan-immune-inflammation value (PIV) are markers of inflammation and immune marker, and there is no study with LCNEC. Therefore, in our study to evaluate the relationship between these markers and LCNEC prognosis; We retrospectively analyzed 109 patients diagnosed with metastatic LCNEC in our center between 2009 and 2022 by calculating the LIPI and PIV values at the time of diagnosis. Median OS was 7.8 (95% Confidence interval (CI), 6.20-9.39) months. Respectively median OS times were 9.6 (95% CI, 7.28-11.91) months versus 4.5 (95% CI, 2.87-6.13) months in low and high LIPI scores. Median OS times were 10.25 months (95 CI, 9.12-11.32) compared to 3.95 months (95 CI, 2.5-6.36) in low and high PIV, respectively. In multivariate analysis ECOG performance score ( p=0.005), metastasis time (de novo vs recurrent) (p=0.008), platelet (p=0.04), albumin (p=0.026), lymphocyte count (p =0.037), LIPI (p =0.002) score and PIV (p =0.001) were related with survival time. In conclusion LIPI, PIV, ECOG performance score, de novo metastases, albumin levels, lymphocyte and platelet counts are associated with prognosis. These factors can be used in patient monitoring and treatment as simple and inexpensive biomarkers.
Complexation of metal ions by propyl gallate and procyanidins: A spectroscopic and quantum chemical calculation method
Authors: Zhang Liangliang , Guan Qinhao , Zhang He 
Published: December 2022

Abstract
The deprotonation mechanism for the phenolic hydroxyl and the complexing of metal ions with a commonly used food additive, propyl gallate (PG) were studied theoretically and experimentally. The interaction of procyanidins [PC, epicatechin16 (4→8) catechin], and its basic monomeric unit catechin (CA) with metal ions was studied by the fluorescence quenching spectra. The results showed that the 9-OH quinoid PG was formed at higher pH (10.9) by the oxidization of phenolic hydroxyl. The binding affinities (Ka) and stoichiometry of these metal ions with PG were determined. The Al3+ in PG-Al complex [Al(PG)(H2O)2Cl2]− was coordinated at the 8,9-OH doubly deprotonated catechol site with double chloride ions (Cl−) and double water molecules (H2O). The fluorescence quenching titration with Sn2+, Zn2+, Cu2+, Al3+ and Fe3+ revealed that the stoichiometries of metal-bound PC were 1:1, 2:3, 2:3, 2:3 and 1:1, respectively. The presence of bovine serum albumin (BSA) could enhance the complexing strength of PC with metal ions.
Network pharmacological analysis of corosolic acid inhibits hepatocellular carcinoma progression through P4HA2
Authors: Tang Feifeng , Liu Long , Tian Xiaoting , Li Ning , Peng Yingxiu , Qian Chunmei , Jia Tingting , Liu Jingjin , Gao Wenhui , Xu Yanfeng 
Published: December 2022

Abstract
Hepatocellular carcinoma (HCC) is a common cancer with very limited therapeutic options. Our previous study revealed that corosolic acid inhibited HCC proliferation and enhanced chemotherapy sensitivity. This study set out to identify the differentially expressed proteins of corosolic acid in the treatment of liver cancer cells, providing molecular targets for targeted therapy of liver cancer in the future. First, data on potential therapeutic targets regulated by corosolic acid were collected using proteomics. The enrichment analysis of GO and KEGG was used to identify the differentially expressed proteins. Differentially expressed genes (DEGs) from The Cancer Genome Atlas (TCGA) liver cancer dataset were analyzed by using the DESeq2 R package. Then,databases such as GEPIA2, Human Protein Atlas, and UALCAN were used to validate the differential expression of DEGs and the prognostic relevance to patients. Finally, experiments were carried out to verify the effect of corosolic acid on hepatocellular carcinoma cell phenotype and the modulation of the screened target proteins. This study will help to understand the molecular changes of HCC after corosolic acid treatment, which will help to find new targets and design effective chemotherapy regimens for future HCC treatment.
Genomics of breast cancer brain metastases: a meta-analysis and therapeutic implications
Authors: Nguyen Thuy Thi , Hamdan Diaddin , Angeli Eurydice , Le Quang Van , Pamoukdjian Frédéric , Bousquet Guilhem 
Published: December 2022

Abstract
Background
Breast cancer brain metastases are challenging daily pratice, and the biological link between gene mutations and metastatic spread to the brain remains to be determined. Here, we performed a meta-analysis on genomic data obtained from primary tumors, extracerebral metastases and brain metastases, to identify gene alterations associated with metastatic processes in the brain.
Methods
Articles with relevant findings were selected using Medline via PubMed, from January 1999 up to February 2022, and the algorithms were the following: ("Breast Neoplasms"[Mesh] AND "metast*" AND ("Genomics"[Mesh] OR "mutation*")), and "Breast" AND "brain" AND "metast*" AND ("Genom*" OR "mutation*" OR "sequenc*"). A critical review was conducted according to the Preferred Reporting Items for Systematic Review and Meta-analysis statement (PRISMA).
Results
Fifty-seven publications were selected for this meta-analysis, including 37,218 patients in all, 11,906 primary tumor samples, 5,541 extracerebral metastasis samples, and 1,485 brain metastasis samples. We report overall and sub-group prevalence of gene mutations, including comparisons between primary tumors, extracerebral metastases and brain metastases. In particular, we identified 6 genes with a higher mutation prevalence in brain metastases than in extracerebral metastases, with a potential role in metastatic processes in the brain: ESR1, ERBB2, EGFR, PTEN, BRCA2 and NOTCH1. We discuss here the therapeutic implications.
Conclusion
Our results underline the added value of obtaining biopsies from brain metastases to fully explore their biology, to develop personalized treatments.
Design of rice with low cadmium accumulation in grain based on single segment substitution line
Authors: Yuan Xue , Liang Ruiqing , Zhou Wenqi , Wang Gan , Liu Na , Gong Yongfu , Ma Shuaipeng , McCouch Susan R. , Chen Leyi , Huang Lilong , Liang Xiaoya , Ba Yuanyuan , Lu Qimiao , Lei Jingtong , Xiao Mengnan , Liang Jianchi , Zhang Guiquan , Wang Shaokui 
Published: December 2022

Abstract
Rice (Oryza sativa L.) is a major source of dietary cadmium (Cd). Developing rice varieties with lower levels of Cd in the grain offers an economical and practical way to improve food safety, especially in areas with high Cd contamination. However, the genetic mechanism of Cd accumulation in rice grain is not fully understood. Here, we identified eight QTLs for Cd accumulation in rice grain based on substitution mapping using single segment substitution lines (SSSLs). The QTLs were located on 7 chromosomes, and named qCd-2-1, qCd-3-1, qCd-3-2, qCd-5-1, qCd-6-1, qCd-7-1, qCd-8-1 and qCd-11-1 accordingly. Two of them, qCd-5-1 and qCd-6-1 are reported here for the first time. The haplotypes of candidate genes associated with heavy metal metabolism, especially Cd accumulation, were analyzed in detail. All SSSLs harbouring alleles from donor parents significantly decreased Cd accumulation with additive effects ranging from − 0.061 to -0.105. Six pyramided lines were developed based on crossing and maker-assisted selection, and these six pyramided lines showed significantly decreased Cd content in grain compared with that of the elite, indica recurrent parent, HJX74, while most of agronomic characteristics of the pyramided lines were similar to HJX74. Taken together, this study demonstrates that identifying and pyramiding QTLs for lower Cd accumulation is an effective strategy for developing rice varieties with lower grain Cd accumulation.
AIBP promotes cell proliferation and migration through MAPK/ERK1/2 signaling pathway in hepatocellular carcinoma
Authors: Huang Tianxin , Ge Sijia , Huang Wei , Ma Tao , Sheng Yu , Chen Jing , Wu Shuzhen , Liu Zhaoxiu , Lu Cuihua 
Published: December 2022

Abstract
Background: Apolipoprotein A-I binding protein (AIBP) is the major apolipoprotein of high-density lipoproteins (HDLs), which plays an important role in cholesterol metabolism and angiogenesis, as well as a variety of inflammation-related diseases, including cancer. However, the roles of AIBP in hepatocellular carcinoma (HCC) remains unclear.
Methods: The expression of AIBP and its relationship with clinical prognosis were analyzed based on The Cancer Genome Atlas (TCGA) database. Western blotting (Wb) and immunohistochemistry (IHC) were used to analyze the expression of AIBP in human HCC tissues. CCK-8 and Colony-formation assays were used to evaluate the abilities of cell proliferation in vitro. Transwell and wound-healing assays were used to assess cell migration and invasion rate. The xenograft tumor model was used to explore the proliferation ability of HCC cells in nude mice.
Results: The expression levels of AIBP were significantly higher in HCC tissues than that in adjacent normal tissues. Patients with high AIBP expression showed poor prognosis. Overexpression of AIBP in SMMC-7721 cells could promote cell proliferation, migration and invasion. Conversely, knockdown of AIBP in HCC-LM3 cells significantly reduced cell proliferation, migration and invasion in vitro. In addition, overexpression of AIBP could promote the proliferation ability of HCC cell in vivo. Finally, we found that AIBP could regulate the expression of MAPK signaling pathway related gene, such as ERK1/2, P-ERK1/2, MEK, P-MEK and c-Myc, and GDC-0994, a specific inhibitor of ERK1/2, could attenuated cell proliferation and migration abilities induced by overexpression of ABIP.
Conclusions: These results suggested that high expression of AIBP in HCC tissues may promote cell proliferation, migration and invasion through MAPK/ERK signaling pathway. AIBP was expected to be a potential marker for early diagnosis and prognosis of HCC.
Longitudinal Assessment of Left Ventricular Function in Patients with Myopericarditis after mRNA COVID-19 Vaccination
Authors: Barresi Nicholas V. , McCollum Sarah , Faherty Erin , Steele Jeremy M. , Karnik Ruchika 
Published: December 2022

Abstract
Background
Multiple reports have described myopericarditis following mRNA COVID-19 vaccination. However, data on the persistence of subclinical myocardial injury assessed by left ventricular (LV) longitudinal strain (LVLS) is limited.
Objectives
Our aim was to assess LV function longitudinally in our cohort of COVID-19 vaccine-related myopericarditis using ejection fraction (EF), fractional shortening (FS), LVLS, and diastolic parameters.
Methods
Retrospective, single-center review of demographic, laboratory, and management data was performed on 20 patients meeting diagnostic criteria for myopericarditis after mRNA COVID-19 vaccination. Echocardiographic images were obtained on initial presentation (time 0), at a median of 12 days (7.5, 18.5; time 1), and at a median of 44 days (29.5, 83.5; time 2). FS was calculated by M-mode, EF by 5/6 area-length methods, LVLS by utilization of TOMTEC software, and diastolic function by tissue Doppler. All parameters were compared across pairs of these time points using Wilcoxon signed-rank test.
Results
Our cohort consisted predominantly of adolescent males (85%) with mild presentation of myopericarditis. The median EF was 61.6% (54.6, 68.0), 63.8% (60.7, 68.3), 61.4% (60.1, 64.6) at times 0, 1, and 2, respectively. Upon initial presentation, 47% of our cohort had LVLS < -18%. The median LVLS was − 18.6% (-16.9, -21.0) at time 0, -21.2% at time 1 (-19.4, -23.5) (p = 0.004) and − 20.8% (-18.7, -21.7) at time 2 (p = 0.004, as compared to time 0).
Conclusions
Though many of our patients had abnormal strain during acute illness, LVLS improved longitudinally, indicating myocardial recovery. LVLS can be used as marker of subclinical myocardial injury and risk stratification in this population.
Antifungal activity of Co(II) and Cu(II) complexes containing 1,3-bis(benzotriazol-1-yl)-propan-2-ol on the growth, virulence traits of fluconazole-resistant Candida: Synthesis, DFT calculations and biological activity
Authors: Murcia-Galán Ricardo A. , Durán Sandra M. , Leal Sandra M. , Roa Martha V. , Vargas Jose D. , Herrera Laura V. , Muñoz-Castro Alvaro , MacLeod-Carey Desmond , Naranjo Tonny W. , Hurtado John J. 
Published: December 2022

Abstract
Relevant virulence traits in Candida are associated with the dimorphic change and biofilm formation, which became an important target to reduce the antifungal resistance. In this work, Co(II) complexes containing a benzotriazole derivative ligand showed a promising capacity of reduce these virulence traits. These complexes exhibited higher antifungal activities than the free ligands against all the Candida albicans and non-albicans strains tested, where compounds 2 and 4 showed minimum inhibitory concentration values between 15.62 and 125 µg mL− 1. Moreover, four complexes (2–5) of Co(II) and Cu(II) with benzotriazole ligand were synthesized. These compounds were obtained as air-stable solids and characterized by melting point, thermogravimetric analysis, infrared, Raman and ultraviolet/visible spectroscopy. The analysis of the characterization data allowed to identify that all the complexes had 1:1 (M:L) stoichiometries. Additionally, Density Functional Theory calculations were carried out for 2 and 3 to propose a probable geometry of both compounds. The conformer Da of 2 was the most stable conformer according to the Energy Decomposition Analysis; while the conformers of 3 have a fluxional behavior in this analysis that didn’t allow to recognize the most probable conformer. These results provide an important platform for the design of new compounds with antifungal activities and capacity of attack other target of relevance to reduce the antimicrobial resistance.
NiTe Magnetic Semiconductor Nanorods for Optical Limiting and Hydrogen peroxide sensor
Authors: Manikandan M. , Manikandan E. , Alshgari Razan A. , Karami Abdulnasse Mahmoud , Ahmad Awais 
Published: December 2022

Abstract
The hydrothermal technique was used to make nickel telluride nanorods (NiTe NRs) utilising ascorbic acid and cetrimonium bromide (CTAB) as reducing agents. Temperature dependent magnetic study for NiTe NRs shows a ferromagnetism behavior. Under 532 nm laser excitation, the obtained materials had a better optical limiting property, with a two photon absorption coefficient of 6.6 ⋅ 10− 10 m/W and an optical limiting of 2.44 J/cm2 at 200 µJ. NiTe NRs modified electrode shows a excellent hydrogen peroxide electrocatalytic activity with reproducibility, repeatability and durability. It displays an outstanding sensitivity of 6.35 µAµM− 1 cm− 2 and a detection limit of 6 nM. In the presence of interfering species such as dopamine, uric acid, ascorbic acid, glucose, and folic acid, the electrode has a high level of selectivity. A real sample analysis for NiTe NRs sensor has been established in human serum and rat brain serum showed good recoveries.
A new composite technique to obtain non-traveling wave solutions of the (2+1)-dimensional extended variable coefficients Bogoyavlenskii-Kadomtsev-Petviashvili equation
Authors: Zheng Xiaoxiao , Zhao Lingling , Xu Yuanqing 
Published: December 2022

Abstract
In this article, we investigate non-traveling wave solutions for the (2+1)-dimensional extended variable coefficients Bogoyavlenskii-Kadomtsev-Petviashvili equation with time-dependent coefficients (VC-BKP). Inspired by Prof. Shang, we apply the extended three-wave method and the generalized variable separation method to the investigated problem for the first time in this article. The technique is effective, easily applicable, and reliable in solving non-traveling wave solutions. We successfully obtain forty-four exact non-traveling solutions, including double periodic solutions, kinky breather wave solution, periodic cross-kink solution and some new exact non-traveling solutions obtained firstly in this paper. These results all have a tail which gives a prediction of physical phenomenon. Moreover, we discuss the arbitrary coefficients of solutions in the real, purely imaginary and complex domains, which greatly enriches the forms of solutions. The dynamic phenomena of four types of exact solutions are demonstrated by contour, 2D and 3D graphics, which help to show their physical interpretation.
2010 Mathematics Subject Classification 35C99, 35G20, 37K10, 68W30