bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGGUNAAN ALAT-ALAT LABORATORIUM UNTUK MENINGKATKAN PEMAHAMAN KONSEP DALAM PEMBELAJARAN IPA DI SMP NEGERI 14 KOYA KOSO JAYAPURA
Authors: Sutarman Tatang , Warpur Maklon 
Published: September 2022

The aims of this service were: (1) To improve the understanding of science’s concept for teachers and pupil; (2) To improve the awereness and interest of students to study science; (3) To improve the soft skill of science teacher to create the simple and interesting learning program. The methods used this time was using a tools gathered around the school. Some equipments from laboratory were also used to support the activity. We can conclude that our activity were success in pulling out the awareness of students to learn sciences. We can measures it from their enthusiasm of the pupils when doing the exercise using gathered tolls and laboratory equipment. Keywords: science, study, equipments, laboratory, Jayapura
Peran Pendidikan IPS Dalam Pembentukan Perilaku Sosial Dan Tanggung Jawab Sosial Era Abad 21
Authors: Muslim Muslim 
Published: September 2022

Tujuan dari kajian ini adalah untuk mengetahui bagaimana peran pendidikan IPS dalam pembentukan perilaku sosial dan tanggung jawab sosial di era abad 21. Metode penelitian kualitatif dengan pendekatan deskriptif dan studi kepustakaan. Hasil kajian ini menunjukkan bahwa: 1) Dalam membentuk perilaku sosial dan tanggung jawab sosial pada diri siswa pada mata pelajaran IPS dapat dikembangkan melalui kompetensi personal, kompetensi sosial dan kompetensi intelektual. 2) Kompetensi sosial yang dikembangkan ialah kesadaran dirinya sebagai anggota masyarakat sehingga perlu saling menghormati dan menghargai; toleransi, pemahaman dan kesadaran atas kesantunan hidup bermasyarakat dan berbangsa; kemampuan berkomunikasi dan kerjasama antara sesama; sikap prososial; kemampuan dan kepedulian sosial pada lingkungan; memperkokoh semangat kebangsaan, pemahaman tentang perbedaan dan kesederajatan. Kajian ini pada akhirnya setelah keterampilan sosial dan kompetensi sosial dikembangkan pada mata pelajaran IPS maka perilaku sosial dan tanggung jawab sosial pada diri siswa dapat terbentuk dengan baik.
KOMPARASI USAHA PEMBESARAN IKAN NILA DI KOLAM TANAH DAN KERAMBA JARING APUNG PADA KOLONG
Authors: Pepayocha Emia , Kurniawan Ardiansyah , Bidayani Endang 
Published: September 2022

Communities on the island of Bangka use soil ponds and floating net cages on ex tin mines lake for tilapia cultivation. Currently, the comparison of the productivity of the two cultivation system for Tilapia cultivation is not known. This study analyzed the business and productivity of tilapia rearing under floating net cages and soil ponds in Air Mesu Village, Pangkalan Baru District, Central Regency. The method used in this study is a survey method with case study techniques with data collection through interviews, questionnaires, and observations. Overall, the business of rearing Tilapia with the KJA system and soil ponds in Air Mesu Village is feasible to develop because the R/C ratio is >1. The use of the KJA system has an R/C ratio and greater productivity than the ground pool. Yield productivity of 25.12 kg/m2 in the KJA system and 19.52/m2 in the soil pond system.
LONG AND WEIGHT RELATIONSHIP TO FECUNDITY OF CEMPEDIK FISH (Osteochilus spilurus) IN CATCHING SEASON IN THE LENGGANG RIVER, EAST BELITUNG
Authors: Kurniawan Ardiansyah , Rizkika Neri , Fakhrurrozi Yulian 
Published: September 2022

The one of Belitung Island’s native fish which has important economic value is Cempedik fish (Osteochilus spilurus). Its consumption, which still relies on natural catchments, requires a study of its biology and ecology. One of the studies that have never been studied in the maturity of this species is the relationship of fecundity with fish weight - length and gonad weight. This study used samples of Cempedik fish from the Lenggang River, East Belitung which were obtained randomly from the catches of fishermen. Sampling adjusts the fishing season that occurs in the rainy season. The preparation was carried out on whole fish using 4% formalin. Calculation of fecundity gravimetrically and compared with fish lengths and gonad weights in the graph. Fecundity in 2015 was 222 – 10,947 eggs and in 2016 there were 335 – 2,060 eggs. Fish body length and weight affected the fecundity of the 2015 fish sample but had no effect on the 2016 sample. This is predicted to occur due to differences in sampling time to position and rainfall in the rainy season, availability and suitability of natural feed, and changes in habitat quality. Gonadal weight correlated with fecundity, where the higher the gonadal weight, the higher the fecundity.
Keywords: Cempedik, rainy season, maturity, fecundity.
Contract Theory, Title Transfer, and Libertarianism
Authors: Dominiak Łukasz , Fegley Tate 
Published: September 2022

In the present paper we argue that the theory of contracts embraced by many libertarian scholars and relied upon by them in sundry important debates (e.g. over morality of the fractional reserve banking or loan maturity mismatching etc.), that is, the title transfer theory of contracts (TTT) should be rejected as not being able to account for the binding force of future-oriented contracts, including contracts deemed enforceable by those scholars themselves. The TTT claims that the only contracts that should be legally binding are these where the debtor’s failure to abide by them constitutes a violation of the creditor’s private property rights. However, as we argue, no default of the debtor in a future-oriented contract can in itself amount to such a violation.
Education level may modify the association between cardiac index and cognitive function among elders with normal ejection function
Authors: Cheng Hao-Min , Chuang Shao-Yuan , Ko Yu-Ting , Liao Chao-Feng , Pan Wen-Harn , Liu Wen-Ling , Hung Chen-Ying , Chen Chen-Huan 
Published: September 2022

BackgroundLower cardiac index (CI) in elders has been associated with incident dementia, and higher CI has protectively effect with brain aging. In the present study, we investigated the modulating effects of education level and arterial stiffness on the association between CI and cognitive function among older adults.MethodsA total of 723 elders (≥60 years, 50.1% women) with normal left ventricular ejection fraction (≥50%) were identified from the Cardiovascular Diseases Risk Factor Two-Township Study. CI was calculated from the Doppler-derived stroke volume. We evaluated arterial stiffness by measuring carotid-femoral pulse wave velocity (CFPWV) and global cognitive function by using the Mini-Mental Short Examination (MMSE). Education level was determined by years of formal education.ResultsIn linear regression analysis adjusting for age, sex, formal years of education, and CFPWV, CI was significantly positively associated with MMSE (BETA=0.344±0.130, P = 0.0082). In logistic regression analysis adjusting for age, sex, formal years of education, and CFPWV, subjects with a CI≥75 percentile had a significantly lower risk of low MMSE (&lt;26) (OR = 0.495, 95% CI = 0.274–0.896, P = 0.02). In subgroup analysis, higher CI was significantly associated with higher MMSE and lower risk of low MMSE only in elders with ≤ 9 years of formal education. Causal mediation analysis suggests that higher CI maintains higher MMSE in elders with lower education levels whereas higher CFPWV causes lower MMSE in all the elders.ConclusionIn elders with normal ejection fraction, a higher CI was associated with a lower risk of cognitive function impairment, independent of arterial stiffness, mainly in subjects with a lower education level and possibly a smaller cognitive reserve.
Association of admission hyperglycemia and all-cause mortality in acute myocardial infarction with percutaneous coronary intervention: A dose–response meta-analysis
Authors: Cheng Shao-Yong , Wang Hao , Lin Shi-Hua , Wen Jin-Hui , Ma Ling-Ling , Dai Xiao-Ce 
Published: September 2022

ObjectiveThe aim of this study is to evaluate the associations between admission hyperglycemia and the risk of all-cause mortality in patients with acute myocardial infarction (AMI) with or without diabetes, to find optimal admission glucose intervention cut-offs, and to clarify the shape of the dose–response relations.MethodsMedline/PubMed and EMBASE were searched from inception to 1 April 2022. Cohort studies reporting estimates of all-cause mortality risk in patients with admission hyperglycemia with AMI were included. The outcomes of interest include mortality and major adverse cardiac events (MACEs). A random effect dose–response meta-analysis was conducted to access linear trend estimations. A one-stage linear mixed effect meta-analysis was used for estimating dose–response curves. Relative risks and 95% confidence intervals were pooled using a random-effects model.ResultsOf 1,222 studies screened, 47 full texts were fully reviewed for eligibility. The final analyses consisted of 23 cohort studies with 47,177 participants. In short-term follow-up, admission hyperglycemia was associated with an increased risk of all-cause mortality (relative risk: 3.12, 95% confidence interval 2.42–4.02) and MACEs (2.34, 1.77–3.09). In long-term follow-up, admission hyperglycemia was associated with an increased risk of all-cause mortality (1.97, 1.61–2.41) and MACEs (1.95, 1.21–3.14). A linear dose–response association was found between admission hyperglycemia and the risk of all-cause mortality in patients with or without diabetes.ConclusionAdmission hyperglycemia was significantly associated with higher all-cause mortality risk and rates of MACEs. However, the association between admission hyperglycemia and long-term mortality risk needs to be determined with caution. Compared with current guidelines recommendations, a lower intervention cut-off and more stringent targets for admission hyperglycemia may be appropriate.Systematic review registration[https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022317280], identifier [CRD42022317280].
Long-term remission of type 2 diabetes after very-low-calorie restriction and related predictors
Authors: Wei Jie , Chen Jie , Wei Xiao , Xiang Xiaoduo , Cheng Qing , Xu Jiechao , Xu Shuhang , Chen Guofang , Liu Chao 
Published: September 2022

PurposeVery low-calorie restriction (VLCR) can induce remission of type 2 diabetes mellitus (T2DM), but its long-term remission and related predictors have not been clarified. The aim of present study is to investigate the effect of VLCR in inducing long-term T2DM remission, and the underlying predictors.MethodsA total of 61 participants with T2DM who received 9 days of VLCR from Dec 2012 to Oct 2020 were followed up in Nov 2021, and divided into responders and non-responders groups. Responders were defined as HbA1c &lt; 6.5% over at least 3 months in the absence of pharmacotherapy. Clinical characteristics were compared between responders and non-responders. Potential predictors were examined by logistic regression analyses based on clinical data before and after VLCR.ResultsForty-four participants were successfully followed up, including 19 males and 25 females. Long-term remission was observed in 17 participants (38.64%) after VLCR, with a median 7.83 years. Compared with non-responders, responders had a shorter disease duration, a lower fasting blood glucose (FBG) level, a higher fasting insulin level, and better HOMA-β after VLCR. Besides, acute insulin response (AIR), insulin area under curve in intravenous and oral glucose tolerance test (IVGTT-IAUC and OGTT-IAUC) in responders were higher than those in non-responders after VLCR. Multivariable logistic analysis showed that higher post-VLCR IVGTT-IAUC predicted a longer T2DM remission.ConclusionsAfter VLCR, more than one third of the participants presented remission over up to 8 years. The improvement of β-cell function, especially the restoration of first-phase insulin-secreting capacity, could prolongate the remission.
Identification and molecular analysis of 11 cases of the PTS gene variants associated with tetrahydrobiopterin deficiency
Authors: Li Lulu , Yang Haihe , Zhao Jinqi , Yang Nan , Gong Lifei , Tang Yue , Kong Yuanyuan 
Published: September 2022

Background: Tetrahydrobiopterin deficiency (BH4D) is a rare autosomal recessive amino acid metabolic disease that belongs to a kind of hyperphenylalaninemia (HPA), and 6-pyruvyltetrahydrotrexate synthase (PTPS) deficiency is the most common type of BH4D. This study investigates the clinical and genetic characteristics of 11 PTPS deficiency cases in the Beijing area, identifies the genetic pathogenic factors, and evaluates the value of high-throughput sequencing in the precise diagnosis of PTPS deficiency.Methods: The Beijing Neonatal Disease Screening Center diagnosed patients with HPA. The study used phenylalanine (Phe) in blood, the ratio of Phe to Thr, urotrexate spectrum analysis, erythrocyte dihydrotrexate reductase (DHPR) activity determination, and high-throughput sequencing as methods. Bioinformatics software analyzed the variants’ pathogenicity and used RT-PCR to identify deep intron variants’ pathogenicity.Result: Among 635 cases with HPA, 38 cases were diagnosed with BH4D, of which the incidence in HPA was 5.98%. Nine kinds of PTS gene variants were detected, including seven missense variants, one splicing variant, and one deletion variant. The splicing variant c.84–291A&gt;G had three splicing results in vivo: normal length, 79bp pseudoexon insertion, and exon 3 skipping. Bioinformatics and Sanger sequencing were performed to verify the identified variants.Conclusion: High-throughput sequencing is a helpful tool for clinical diagnosis and differential diagnosis of BH4D. This study confirms that c.84–291A&gt;G is the hot spot variant of PTPS deficiency, and it is the first reported variant with a new splicing pattern in vivo. A novel deletion variant c.84_163del (p.Lys29Cysfs∗9) was found to enrich the genetic variant spectrum of the disease.
Comprehensive analysis of the expression of N6-methyladenosine RNA methylation regulators in pulmonary artery hypertension
Authors: Zheng Hao , Hua Jing , Li Hongpeng , He Wenjuan , Chen Xiangyu , Ji Yingqun , Li Qiang 
Published: September 2022

Background: Pulmonary arterial hypertension (PAH) is a progressive disease characterized by pulmonary vascular remodeling. The development of PAH involves N6-methyladenosine (m6A) modification. However, the functional role of m6A regulators in PAH and the underlying regulatory mechanisms remain unknown so far.Methods: Microarray data (GSE149713) for monocrotaline induced PAH (MCT-PAH) rat models were downloaded and screened for differentially expressed genes (DEGs) and m6A regulators. Next, we screened for differentially expressed m6A regulators in endothelial cells (ECs), smooth muscle cells (SMCs), fibroblasts, interstitial macrophages, NK cells, B cells, T cells, regulatory T cells (Tregs) using scRNA sequencing data. The target DEGs of m6A regulators in ECs, SMCs, fibroblasts, and Tregs were functionally annotated using the Gene Ontology (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In addition, the cellular interaction analysis was performed to reveal the receptor—ligand pairs regulated by m6A regulators. Pseudo-time trajectory analyses were performed and a ceRNA network of lncRNAs-miRNAs-mRNAs was constructed in SMCs. Furthermore, the RNA transcriptome sequencing data for the SMCs isolated from idiopathic PAH (IPAH) patients (GSE144274) were validated for differentially expressed m6A regulators. Moreover, the HNRNPA2B1 levels in the lung samples from PAH patients and MCT-PAH were determined using immunohistochemistry.Results: The m6A regulators were observed to be dysregulated in PAH. HNRNPA2B1expression level was increased in the PASMCs of scRNAs and IPAH patients. The target DEGs of HNRNPA2B1 were enriched in the regulation of muscle cell differentiation and vasculature development in PASMCs. The HNRNPA2B1 expression levels determined were consistent with the proliferation-related and collagen synthesis-related gene COL4A1. Moreover, the predicted transcription factors (TFs) foxd2/3 and NFκB could be involved in the regulation of HNRNPA2B1. HNRNPA2B1 might be regulating SMCs proliferation and phenotypic transition via rno-miR-330–3p/TGFβR3 and rno-miR-125a-3p/slc39a1. In addition, HNRNPA2B1 was observed to be highly expressed in the lung samples from MCT-PAH rat models and patients with PAH.Conclusion: In summary, the present study identified certain key functional m6A regulators that are involved in pulmonary vascular remodeling. The investigation of m6A patterns might be promising and provide biomarkers for diagnosis and treatment of PAH in the future.
HDL metabolism and functions impacting on cell cholesterol homeostasis are specifically altered in patients with abdominal aortic aneurysm
Authors: Adorni Maria Pia , Palumbo Marcella , Marchi Cinzia , Zimetti Francesca , Ossoli Alice , Turri Marta , Bernini Franco , Hollan Ivana , Moláček Jiří , Treska Vladislav , Ronda Nicoletta 
Published: September 2022

BackgroundThe etiopathogenesis of abdominal aortic aneurysm (AAA) is still unclarified, but vascular inflammation and matrix metalloproteases activation have a recognized role in AAA development and progression. Circulating lipoproteins are involved in tissue inflammation and repair, particularly through the regulation of intracellular cholesterol, whose excess is associated to cell damage and proinflammatory activation. We analyzed lipoprotein metabolism and function in AAA and in control vasculopathic patients, to highlight possible non-atherosclerosis-related, specific abnormalities.MethodsWe measured fluorometrically serum esterified/total cholesterol ratio, as an index of lecithin-cholesterol acyltransferase (LCAT) activity, and cholesteryl ester transfer protein (CETP) activity in patients referred to vascular surgery either for AAA (n=30) or stenotic aortic/peripheral atherosclerosis (n=21) having similar burden of cardiovascular risk factors and disease. We measured high-density lipoprotein (HDL)-cholesterol efflux capacity (CEC), through the ATP-binding cassette G1 (ABCG1) and A1 (ABCA1) pathways and serum cell cholesterol loading capacity (CLC), by radioisotopic and fluorimetric methods, respectively.ResultsWe found higher LCAT (+23%; p &lt; 0.0001) and CETP (+49%; p &lt; 0.0001) activity in AAA sera. HDL ABCG1-CEC was lower (−16%; p &lt; 0.001) and ABCA1-CEC was higher (+31.7%; p &lt; 0.0001) in AAA. Stratification suggests that smoking may partly contribute to these modifications. CEC and CETP activity correlated with CLC only in AAA.ConclusionsWe demonstrated that compared to patients with stenotic atherosclerosis, patients with AAA had altered HDL metabolism and functions involved in their anti-inflammatory and tissue repair activity, particularly through the ABCG1-related intracellular signaling. Clarifying the relevance of this mechanism for AAA evolution might help in developing new diagnostic parameters and therapeutic targets for the early management of this condition.
IL36G is associated with cutaneous antiviral competence in psoriasis
Authors: Lu You-Wang , Chen Yong-Jun , Shi Nian , Yang Lu-Hui , Wang Hong-Mei , Dong Rong-Jing , Kuang Yi-Qun , Li Yu-Ye 
Published: September 2022

BackgroundPsoriasis is a common inflammatory skin disease that has a great impact on patients’ physical and mental health. However, the causes and underlying molecular mechanisms of psoriasis are still largely unknown.MethodsThe expression profiles of genes from psoriatic lesion samples and skin samples from healthy controls were integrated via the sva software package, and differentially expressed genes (DEGs) between psoriasis and healthy skin were screened by the limma package. Furthermore, GO and KEGG pathway enrichments for the DEGs were performed using the Clusterprofiler package. Protein–protein interaction (PPI) networks for the DEGs were then constructed to identify hub genes. scGESA analysis was performed on a single-cell RNA sequencing dataset via irGSEA. In order to find the cytokines correlated with the hub genes expression, single cell weighted gene co-expression network analyses (scWGCNA) were utilized to build a gene co-expression network. Furthermore, the featured genes of psoriasis found in suprabasal keratinocytes were intersected with hub genes. We then analyzed the expression of the intersection genes and cytokines in the integrated dataset. After that, we used other datasets to reveal the changes in the intersection genes’ expression levels during biological therapy. The relationship between intersection genes and PASI scores was also explored.ResultsWe identified 148 DEGs between psoriatic and healthy samples. GO and KEGG pathway enrichment analysis suggested that DEGs are mainly involved in the defense response to other organisms. The PPI network showed that 11 antiviral proteins (AVPs) were hub genes. scGSEA analysis in the single-cell transcriptome dataset showed that those hub genes are highly expressed in keratinocytes, especially in suprabasal keratinocytes. ISG15, MX1, IFI44L, and IFI27 were the characteristic genes of psoriasis in suprabasal keratinocytes. scWGCNA showed that three cytokines—IL36G, MIF, and IL17RA—were co-expressed in the turquoise module. Only interleukin-36 gamma (IL36G) was positively correlated with AVPs in the integrated dataset. IL36G and AVPs were found co-expressed in a substantial number of suprabasal keratinocytes. Furthermore, we found that the expression levels of IL36G and the 4 AVPs showed positive correlation with PASI score in patients with psoriasis, and that these levels decreased significantly during treatment with biological therapies, but not with methotrexate.ConclusionIL36G and antiviral proteins may be closely related with the pathogenesis of psoriasis, and they may represent new candidate molecular markers for the occurrence and severity of psoriasis.