bluuebunny/crossref_metadata_2025_split huggingface dataset:
Color stability and translucency of ceramic laminate veneer restoration for diastema closure: Effect of resin cement containing different photoinitiators
Authors: Adel Eman , Al-Zordk Walid , Hasouna Mahy , Elsherbini Ahmed , Sakrana Amal Abdelsamad 
Published: May 2022

Abstract
Objectives: To investigate the color stability and translucency of different ceramic laminate veneers for diastema closure bonded with resin cement using different photoinitiating systems based on benzoyl germanium compounds and iodonium salts.Materials and Method: Sixty abutments were milled from a polymethyl methacrylate disc to duplicate a maxillary left central incisor tooth model that had been prepared for laminate veneer restoration. The abutments were devided into six groups (n=10) according to the ceramic type (lithium disilicate, zirconia reinforced lithium silicate, and translucent zirconia) and cement type (Variolink Esthetic LC and RelyX Veneer). Each laminate veneer was bonded to its respective abutment and stored in water for 24 hours. The color coordinates and translucency were analyzed with a reflectance spectrophotometer after cementation and after immersion in coffee solution. The translucency parameters and contrast ratio were measured. The color and the translucency differences were calculated and the data were analyzed (α=.05). The differences in color and translucency were estimated, and the results were statistically assessed (=.05). Results: Ceramic materials showed a significant impact (P ˂.001) on the color changes after coffee immersion within the Variolink Esthetic LC groups. Ttranslucent zirconia showed the highest color change, followed by zirconia reinforced lithium silicate and lithium disilicate. Among the RelyX Veneer groups, the ceramic materials showed a significant impact (P ˂.001). Translucent zirconia showed the highest color change, followed by zirconia reinforced lithium silicate and lithium disilicate. The two-way ANOVA test showed a significant interaction in color changes between ceramic type and cement type after cementation (P=.040), and after coffee immersion (P=.001). All groups showed a clinically acceptable difference in translucency parameters after coffee immersion. The two-way ANOVA test found a non-significant effect (P=.151) on translucency changes.Conclusions: Cement selection is important for the color stability and translucency of ceramic laminate veneers after coffee staining. Lithium disilicate veneer is more resistant to coffee staining than zirconia-reinforced lithium silicate and translucent zirconia veneers. Ceramic laminate veneers bonded with resin cement contain benzoyl germanium compounds (Variolink Esthetic LC) are more translucency. However, ceramic laminate veneers bonded using resin cement contain iodonium salts (RelyX Veneer) are more resistant to coffee staining. Clinical relevance: The dentists have to face challenges of predicting the final color of various ceramic laminate veneer restorations used for diastema closure and estimating its color stability after final cementation.
Correlation between estimated glucose disposal rate and in-stent restenosis following percutaneous coronary intervention in individuals with non-ST-segment elevation acute coronary syndrome
Authors: Liu Chi , Zhao Qi , Ma Xiaoteng , Cheng Yujing , Sun Yan , Zhang Dai , Liu Xiaoli , Zhou Yujie 
Published: May 2022

Abstract
Background: Insulin resistance (IR) is closely associated with in-stent restenosis (ISR) following percutaneous coronary intervention (PCI). Nevertheless, the predictive power of the newly developed simple assessment of IR, estimated glucose disposal rate (eGDR), for ISR after PCI in individuals with non-ST-segment elevation acute coronary syndrome (NSTE-ACS) remains unclear.Methods: Based on eligibility criteria, 1218 NSTE-ACS cases administered PCI in Beijing Anzhen Hospital Between January and December 2015 were included. The included individuals were submitted to at least one coronary angiography within 48 months after discharge. Patients were assigned to 2 groups according to ISR occurrence or absence. eGDR was derived as 21.16 - (0.09 * waist circumference [cm]) - (3.41 * hypertension) - (0.55 * glycated hemoglobin [%]). Multivariate logistic regression analysis model and receiver operating characteristic (ROC) curve analysis was established for evaluating eGDR’s association with ISR. Results: In multivariate logistic analysis, the odds ratios (ORs) of eGDR as a nominal variate and a continuous variate were 3.393 (confidence interval [CI] 2.099-5.488, P<0.001) and 1.210 (CI 1.063 - 1.378, P=0.004), respectively. The incremental effect of eGDR on ISR prediction based on traditional cardiovascular risk factors was reflected by ROC curve analysis (AUC: baseline model + eGDR 0.644 vs. baseline model 0.609, P for comparison=0.013), continuous net reclassification improvement (continuous-NRI) of -0.264 (p<0.001) and integrated discrimination improvement (IDI) of 0.071 (p=0.065).Conclusion: In NSTE-ACS cases administered PCI, eGDR levels are independently negatively associated with increased ISR risk.
One-Stage Transanal Endorectal Pull-through for Hirschsprung Disease: experience with 229 neonates
Authors: Zhang Yanan , Liu Zhaozhou , Li Shuangshuang , Yang Shen , Zhao Jiawei , Yang Ting , Li Siqi , Chen Yongwei , Guo Weihong , Hou Dawei , Li Yingzi , Huang Jinshi 
Published: May 2022

Abstract
Objective: To evaluate the safety and efficacy of the transanal endorectal pull-through (TEPT) and the long-term outcomes in newborns with HD.Methods: A total of 229 newborns with HD underwent one-stage TEPT between 2007 and 2020, and the diagnoses were confirmed by rectal biopsy. The perioperative clinical course for all patients was reviewed, and the postoperative short- and long-term outcomes were assessed.Results: A total of 229 neonates (187 male and 42 female) had a median age at TEPT of 17 days (range, 6-28 days). Sixty-eight patients (29.7%) underwent TEPT combined with an abdominal approach or laparoscopy. Early postoperative complications were documented in 26 patients (11.4%). The follow-up period in the remaining 165 children ranged from 1.2 to 14.0 years (median, 5.0 years), and late postoperative complications were noted in 6 patients (3.6%). A total of 106 of the patients older than four years old took part in an interview about bowel function, and 85 patients (80.2%) had a bowel function scores (BFS) ≥ 18.Conclusion: TEPT is effective and safe in the treatment of neonatal HD and presents with a low rate of complications and an acceptable outcome.
Increased genital mucosal cytokines in Canadian women associates with increased antigen presenting cells, inflammatory metabolites, epithelial barrier disruption and the depletion of L. crispatus
Authors: Zuend Christina Farr , Lamont Alana , Noel-Romas Laura , Knodel Samantha , Birse Kenzie , Kratzer Kateryna , McQueen Peter , Perner Michelle , Ayele Hossaena , Mutch Sarah , Berard Alicia R. , Schellenberg John J. , Senturk Faruk , McCorrister Stuart , Westmacott Garrett , Mulhall Fran , Sandberg Bonnie , Yu Adelicia , Burnett Margaret , Poliquin Vanessa , Burgener Adam D. 
Published: May 2022

Abstract
Background: Cervicovaginal inflammation has been linked to negative reproductive health outcomes including acquisition of HIV, other sexually transmitted infections, and cervical carcinogenesis. While changes to the vaginal microbiome have been linked to genital inflammation, the molecular relationships between the functional components of the microbiome with cervical immunology in the reproductive tract are understudied, limiting our understanding of mucosal biology that may be important for reproductive health.Results: In this study we used a multi’-omics approach to profile cervicovaginal samples collected from 43 Canadian women to characterize host, immune, functional microbiome and metabolome features of cervicovaginal inflammation. We demonstrate that inflammation is associated with lower amounts of L. crispatus and increased levels of cervical antigen presenting cells (APCs). Proteomic analysis showed an upregulation of pathways related to neutrophil degranulation, complement, and leukocyte migration, with decreased levels of cornified envelope and cell-cell adherens junctions. Functional microbiome analysis showed reductions to carbohydrate metabolism and lactic acid, with increases of xanthine and other metabolites. Bayesian network analysis linked L. crispatus with glycolytic and nucleotide metabolism, succinate and xanthine, and epithelial proteins SCEL and IVL as major molecular features associated with pro-inflammatory cytokines and increased APCs.Conclusions: This study identified key molecular and immunological relationships with cervicovaginal inflammation, including increased APCs, bacterial metabolism, and proteome alterations that underlie inflammation. As APCs are involved in HIV transmission, parturition, and cervical cancer progression, further studies are needed to explore the interactions between these cells, bacterial metabolism, mucosal immunity, and their relationship to reproductive health.
m6A RNA Methylation Regulators de fine tumor microenvironment and predict prognosis in hepatocellular carcinoma
Authors: Gu Xiangqian , Yuan Yin , Gu Chengyu , Wu Mingyu , Cai Bin 
Published: May 2022

Abstract
N6-methyladenosine (m6A) modification is considered to be the most significant and abundant mRNA modification and constitutes a critical mechanism of epigenetic regulation in various tumors. However, the potential influences of these m6A regulators on the tumor immune microenvironment (TIME) and immunotherapy in hepatocellular carcinoma (HCC) remain unknown. The present research aims to explore the roles of 38 m6A regulators in HCC. We investigated the different expression patterns of the m6A regulators between normal and HCC samples. Through consensus clustering of m6A regulators, three subgroups associated with patient survival and the infiltration of immune cells were recognized. Additionally, we constructed an m6A score model based on the m6A phenotype-related differentially expressed genes (DEGs) to quantify the m6A-related subtypes of individual tumors. Patients with a low m6Ascore had a better survival rate than those with a high m6Ascore, and patients with a high TMB and high m6Ascore had a worse survival rate. Moreover, patients in the high m6Ascore group had higher PD-L1 expression than the low m6Ascore group. The low m6Ascore group showed higher immune checkpoint inhibitor responses, including anti-CTLA-4 therapy, anti-PD-1, or a combination of both, than the high m6Ascore group. In conclusion, our study revealed the potential function of these mRNA modifications in TIME and identified their immunotherapeutic value.
Forward masking in a bottlenose dolphin Tursiops truncatus: Dependence on azimuthal positions of the masker and test sources
Authors: Popov Vladimir V. , Nechaev Dmitry I. , Supin Alexander Ya. , Sysueva Evgeniya V. 
Published: May 2022

Abstract
Forward masking was investigated by the auditory evoked potentials (AEP) method in a bottlenose dolphin Tursiops truncatus using stimulation by two successive acoustic pulses (the masker and test) projected from spatially separated sources. The positions of the two sound sources either coincided with or were symmetrical relative to the head axis at azimuths from 0 to ±90°. AEPs were recorded either from the vertex or from the lateral head surface next to the auditory meatus. In the last case, the test source was ipsilateral to the recording side, whereas the masker source was either ipsi- or contralateral. For lateral recording, AEP release from masking (recovery) was slower for the ipsi- than for the contralateral masker source position. For vertex recording, AEP recovery was equal both for the coinciding positions of the masker and test sources and for their symmetrical positions relative to the head axis. The data indicate that at higher levels of the auditory system of the dolphin, binaural convergence makes the forward masking nearly equal for ipsi- and contralateral positions of the masker and test.
Nerolidol induced apoptosis in human laryngeal cancer cells (Hep 2) through activation of ROS and mitochondrial-mediated pathway: An in vitro and in silico study
Authors: Balakrishnan Vaitheeswari , Ganapathy Sindhu , Veerasamy Vinothkumar , Duraisamy Ramachandhiran , Jawaharlal Saranya , Lakshmanan Vennila 
Published: May 2022

Abstract
The aim of the present study was to examine Nerolidol (NER) and cisplatin (CIS) performed against human laryngeal carcinoma (Hep 2) cells. The MTT assay was used to assess the cytotoxicity of NER and CIS on Hep 2 cells in terms of morphological alterations. We evaluated the effect of NER, CIS, and NER + CIS on cell migration, oxidative stress (ROS), mitochondrial membrane depolarization (MMP), nuclear condensation, induction of apoptosis and DNA damage in Hep 2 cells. In addition, the molecular docking study also evaluated the structural binding interaction of the NER with the P13K/Akt and PCNA protein. The results demonstrate that IC50 values of NER and CIS and combination NER + CIS have a potential cytotoxicity against Hep 2 cells. Moreover, effectively inhibit cell viability and increased ROS generation due to induction of apoptosis in Hep 2 cells. Furthermore, the NER interaction with the PI3K/Akt and PCNA protein has a higher binding score and a high binding affinity. As a result of these findings, NER may be a promising anticancer drug in oral squamous cell carcinomas (OSCCs) by inducing apoptosis in Hep 2cells.
Chromosome-scale assembly of the lablab genome - A model for inclusive orphan crop genomics
Authors: Njaci Isaac , Waweru Bernice , Kamal Nadia , Muktar Meki , Fisher David , Gundlach Heidrun , Muli Collins , Muthui Lucy , Maranga Mary , Kaimenyi Davies , Maass Brigitte , Emmrich Peter , Entfellner Jean-Baka Domelevo , Spannagl Manuel , Chapman Mark , Shorinola Oluwaseyi , Jones Chris 
Published: May 2022

Abstract
Orphan crops (also described as underutilised and neglected crops) hold the key to diversified and climate-resilient food systems. After decades of neglect, the genome sequencing of orphan crops is gathering pace, providing the foundations for their accelerated domestication and improvement. Recent attention has however turned to the gross under-representation of researchers in Africa in the genome sequencing efforts of their indigenous orphan crops. Here we report a radically inclusive approach to orphan crop genomics using the case of Lablab purpureus (L.) Sweet (syn. Dolichos lablab, or hyacinth bean) – a legume native to Africa and cultivated throughout the tropics for food and forage. Our Africa-led South-North plant genome collaboration produced a high-quality chromosome-scale assembly of the lablab genome – the first chromosome-scale plant genome assembly locally produced in Africa. We also re-sequenced cultivated and wild accessions of lablab from Africa confirming two domestication events and examined the genetic diversity in lablab germplasm conserved in Africa. Our approach provides a valuable resource for lablab improvement and also presents a model that could be explored by other researchers sequencing indigenous crops particularly from Low and middle income countries (LMIC).
Expression of striated muscle Z-band alternatively spliced PDZ-motif protein LIM domain-binding 3 in the nervous system
Authors: Blech-Hermoni Yotam , Subedi Kalpana , Silver Maya , Jensen Leah , Coscia Stephen , Kates Malcolm M. , Zhao Yongmei , Raley Castle , Edwards Nancy , Tran Bao , Ray-Chaudhary Abhik , Pathak Pankaj , Mankodi Ami 
Published: May 2022

Abstract
LIM domain-binding 3 (LDB3) is a member of the Enigma family of PDZ-LIM proteins. LDB3 has been reported as a striated muscle-specific Z-band alternatively spliced protein that plays an important role in mechanosensory actin cytoskeleton remodeling. This study shows that LDB3 is broadly expressed in the central and peripheral nervous system of human and mouse. LDB3 is predominantly expressed in the adult stages compared to early development and at a significantly higher level in the spinal cord than in the brain. As in skeletal muscle and heart, LDB3 is extensively alternatively spliced in the neurons. Three novel splice isoforms were identified suggesting splicing-dependent regulation of LDB3 expression in the nervous system. Expression of LDB3 in the motor cortex, cerebellum, spinal motor neuron, peripheral nerve, and neuromuscular junction in addition to skeletal muscle indicates important roles for this PDZ-LIM family protein in motor planning and execution. Moreover, expression in the hippocampal neurons suggests roles for LDB3 in learning and memory. LDB3 interactors filamin C and myotilin are also expressed in the spinal motor neuron, nerve, and neuromuscular junction, thereby providing the basis for neurogenic manifestations in myopathies associated with mutations in these so-called muscle proteins.
Genetic control of rhizosphere microbiome of cotton plant under field conditions
Authors: Wei Feng , Feng Zili , Yang Chuanzhen , Zhao Lihong , Zhang Yalin , Feng Hongjie , Zhu Heqin , Xu Xiangming 
Published: May 2022

Abstract

Background
Understanding the extent of heritability of plant-associated microbiome (phytobiome) is critical for exploitation of phytobiome in agriculture. Two crosses were made between pairs of cotton cultivars with differential resistance to the Verticillium wilt. F2 plants were grown together with the four parents to study the heritability of cotton rhizosphere microbiome. Amplicon sequencing was used to profile bacterial and fungal communities.
Results
F2 hybrid offspring of both crosses had higher alpha diversity indices as well as greater variability than the two parents. Two types of data were used generated to study the heritability of rhizosphere microbiome: principal components (PCs) and individual top microbial OTUs. For the L1×Z49 cross, the variance among the 100 progeny genotypes (VT) was significantly greater than the random variability (VE) for 12 and 34 out of top 100 fungal and bacterial PCs, respectively. For the Z2×J11 cross, the corresponding values were 10 and 20 PCs. Out of the top 100 OTUs, 29 fungal OTUs and 10 bacterial OTUs had VT that was significantly greater than VE for the L1×Z49 cross; the corresponding value for the Z2×J11 cross were 24 and one. The estimated heritability was mostly in the range of 40–60%.
Conclusions
These results suggested the existence of genetic control in terms of rhizosphere microbiome in cotton.
Predictors of the Acceptance of an Electronic Coach Targeting Self-management of Patients With Type 2 Diabetes: Web-Based Survey
Authors: Harakeh Zeena , Van Keulen Hilde , Hogenelst Koen , Otten Wilma , De Hoogh Iris M , Van Empelen Pepijn 
Published: May 2022

Background
Type 2 diabetes (T2D) is a lifestyle-related disease whose prevalence increases with age. Diabetes self-management through mobile health (mHealth) apps enables patients with T2D to improve their health. According to the Technology Acceptance Model (TAM), technology acceptance (ie, intended use) is necessary to ensure mHealth can be implemented successfully. Therefore, the specific acceptance requirements of patients with T2D should be considered.

Objective
This cross-sectional study aims to examine the extent to which different TAM predictors are associated with the acceptance of a diabetes app including an electronic coach (eCoach; Iris app) among patients with T2D.

Methods
Using a web-based survey, data on 92 patients with T2D (mean age 62.76 years, SD 8.29 years) were collected. Acceptance of the Iris app with the TAM predictors (ie, perceived usefulness, perceived ease of use, social influence, perceived self-efficacy, perceived security, prior usage experience, perceived health, and propensity of data/information sharing) was assessed. Further, control variables (ie, gender, age, education, ethnicity, household, BMI, amount of years with diabetes, diabetes-related complaints, and medication use) were assessed.

Results
Multiple linear regression analyses showed that acceptance of the Iris app was positively associated with perceived usefulness (β=.57, P<.001), social influence (subjective norm; β=.20, P=.004), and willingness to share data (β=.25, P<.001). In addition, acceptance regarding the Iris app was higher among patients with T2D with overweight (β=.23, P=.01) or obese BMI (β=.21, P=.01). The model explained 75.8% of the variance in the acceptance of the Iris app by patients with T2D. In addition, perceived usefulness of the Iris app was positively related to perceived ease of use (β=.32, P<.001), subjective norm (β=.26, P=.004), perceived control (β=.19, P=.03), willingness to share data (β=.20, P=.01) regarding the Iris app, and perceived security regarding general use of apps/smartphone/internet (β=.15, P=.04). The model explained 58.2% of the variance in patients’ perceived usefulness about the Iris app.

Conclusions
Among patients with T2D, the belief that the use of the Iris app is helpful/beneficial, the willingness to share their Iris app data, and others’ approval of using this app can stimulate the acceptance of this app. In addition, the belief that the use of (health) apps is reliable and secure, the belief that the use of the Iris app is easy to use, a higher perceived capability and personal control with using this app, the willingness to share their Iris app data, and others’ approval of using this app can stimulate the perceived usefulness of such an app. These TAM predictors explained a high variance in acceptance and perceived usefulness of the Iris app. Implications for practice are addressed.
An economic and health outcome evaluation of telehealth in rural sepsis care: a comparative effectiveness study
Authors: Mohr Nicholas M , Schuette Allison R , Ullrich Fred , Mack Luke J , DeJong Katie , Camargo Jr Carlos A , Zachrison Kori S , Boggs Krislyn M , Skibbe Adam , Bell Amanda , Pals Mark , Shane Dan M , Carter Knute D , Merchant Kimberly AS , Ward Marcia M 
Published: May 2022

Aim:
Sepsis is a top contributor to in-hospital mortality and, healthcare expenditures and telehealth have been shown to improve short-term sepsis care in rural hospitals. This study will evaluate the effect of provider-to-provider video telehealth in rural emergency departments (EDs) on healthcare costs and long-term outcomes for sepsis patients.
Materials & methods:
We will use Medicare administrative claims to compare total healthcare expenditures, mortality, length-of-stay, readmissions, and category-specific costs between telehealth-subscribing and control hospitals.
Results:
The results of this work will demonstrate the extent to which telehealth use is associated with total healthcare expenditures for sepsis care.
Conclusion:
These findings will be important to inform future policy initiatives to improve sepsis care in rural EDs.

Clinical Trial Registration:
NCT05072145
(
ClinicalTrials.gov
)