bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unmet clinical needs and burden of disease in hidradenitis suppurativa: real‐world experience from EU5 and US
Authors: Ingram J.R. , Bettoli V. , Espy J.I. , Kokolakis G. , Martorell A. , Villani A.P. , Wallinger H. , Coak E. , Kasparek T. , Muscianisi E. , Richardson C. , Kimball A.B. 
Published: April 2022

AbstractBackgroundHidradenitis suppurativa (HS) is a chronic, inflammatory, debilitating skin disease characterized by painful deep lesions and associated with substantial disease burden.ObjectivesThe objective of this study was to describe physician‐ and patient‐reported clinical unmet needs from a real‐world perspective.MethodsThis study used data from the Adelphi HS Disease Specific Programme, a point‐in‐time survey of dermatologists and their patients with HS in Europe and the United States. Dermatologists completed patient record forms (PRFs) for 5–7 consecutively consulting patients with HS; patients or carers of patients also optionally completed a patient/carer self‐completion questionnaire (PSC/CSC). Data collection included demographics, symptomatology and impact on quality of life (QoL).ResultsDermatologists (N = 312) completed PRFs for 1787 patients with HS; patient‐ and carer‐reported questionnaires (PSC/CSC) were completed for 33.1% (591/1787) of patients. The mean age was 34.4 ± 12.2 years and 57.6% of patients were female (1029/1787). Physician‐judged disease severity at sampling was categorized as mild in 66.0% (1179/1787), moderate in 29.3% (523/1787) and severe in 4.7% (85/1787) of patients. Deterioration or unstable condition over the previous 12 months was described by 17.1% [235/1372] and 12.6% [41/325] of physician‐ and patient/carer‐reported cases, respectively. Despite receiving treatment, high proportions of patients still experienced symptoms at sampling (general pain/discomfort [49.5%, 885/1787]; inflammation/redness of lesions/abscesses [46.1%, 823/1787] and itching [29.9%, 535/1787]); these symptoms were more frequent in patients with moderate or severe disease. Patients reported a mean Dermatology Life Quality Index score of 5.9 ± 5.4 (555/591; mild, 4.1 ± 4.3; moderate, 9.4 ± 5.4; severe, 13.3 ± 5.5) and a mean Hidradenitis Suppurativa Quality of Life score of 11.0 ± 10.6 (518/591; mild, 7.6 ± 8.3; moderate, 17.7 ± 10.0; severe, 31.0 ± 15.4) indicating a substantial impact on QoL.ConclusionsPatients with HS experienced a high disease burden despite being actively treated by a dermatologist. This study demonstrates that the burden of HS disease is generally poorly managed with a considerable impact observed on patients' QoL.
Quinoline, with the active site of 8‐hydroxyl, efficiently inhibits Micropterus salmoides rhabdovirus (MSRV) infection in vitro and in vivo
Authors: Li Bo‐Yang , Yang Fei , Zhang Zhong‐Yu , Shen Yu‐Feng , Wang Tao , Zhao Liang , Qin Jia‐Cheng , Ling Fei , Wang Gao‐Xue 
Published: April 2022

AbstractMicropterus salmoides rhabdovirus (MSRV) is an significant pathogen that causes high mortality and related economic losses in bass aquaculture. There is no effective or approved therapy to date. In this study, we evaluated the anti‐MSRV effects of 22 quinoline derivatives in grass carp ovary (GCO) cells. Among these compounds, 8‐hydroxyquinoline exhibited valid inhibition in decreasing MSRV nucleoprotein gene expression levels of 99.3% with a half‐maximal inhibitory concentrations (IC50) value of 4.66 μM at 48 h. Moreover, 8‐hydroxyquinoline significantly enhanced a protective effect in GCO cells by reducing the cytopathic effect (CPE). By comparing the anti‐MSRV activity of 22 quinoline derivatives, we found that 8‐hydroxyquinoline possessed the efficient active site of 8‐hydroxyl and inhibited MSRV infection in vitro. For in vivo studies, 8‐hydroxyquinoline via intraperitoneal injection exhibited an antiviral effect in MSRV‐infected largemouth bass by substantially enhancing the survival rate by 15.0%. Importantly, the viral loads in the infected largemouth bass notably reduced in the spleen on the third days post‐infection. Overall, 8‐hydroxyquinoline was considered to be an efficient agent against MSRV in aquaculture.
EXPLICIT‐Kinase: A gene expression predictor for dissecting the functions of the Arabidopsis kinome
Authors: Peng Yuming , Zuo Wanzhu , Zhou Hui , Miao Fenfen , Zhang Yu , Qin Yue , Liu Yi , Long Yu , Ma Shisong 
Published: April 2022

ABSTRACTProtein kinases regulate virtually all cellular processes, but it remains challenging to determine the functions of all protein kinases, collectively called the “kinome”, in any species. We developed a computational approach called EXPLICIT‐Kinase to predict the functions of the Arabidopsis kinome. Because the activities of many kinases can be regulated transcriptionally, their gene expression patterns provide clues to their functions. A universal gene expression predictor for Arabidopsis was constructed to predict the expression of 30,172 non‐kinase genes based on the expression of 994 kinases. The model reconstituted highly accurate transcriptomes for diverse Arabidopsis samples. It identified the significant kinases as predictor kinases for predicting the expression of Arabidopsis genes and pathways. Strikingly, these predictor kinases were often regulators of related pathways, as exemplified by those involved in cytokinesis, tissue development, and stress responses. Comparative analyses revealed that portions of these predictor kinases are shared and conserved between Arabidopsis and maize. As an example, we identified a conserved predictor kinase, RAF6, from a stomatal movement module. We verified that RAF6 regulates stomatal closure. It can directly interact with SLAC1, a key anion channel for stomatal closure, and modulate its channel activity. Our approach enables a systematic dissection of the functions of the Arabidopsis kinome.
The function of nuclear hormone receptor 4A signaling in the human reproductive system: A review
Authors: Wang Boya , He Yingming , Zhang Pin , Huang Yue , Xiang Huifen 
Published: April 2022

AbstractAimThis review aims to summarize the research focused upon the functions of nuclear hormone receptor 4A (NR4A) in the human reproductive system. The research questions addressed are to decipher what role the NR4A subfamily plays in the regulation of the human reproductive system and effects upon fertility issues through regulation of the expression of the NR4A subfamily.MethodsThe electronic database PubMed was searched for studies published before November 2021. Keywords included “NR4A,” “trophoblast,” “decidualization,” “folliculogenesis,” “estrogen,” “pregnancy,” “Leydig cells,” “fertility,” and “reproductive.” Relevant references from retrieved manuscripts and review articles were also searched manually.ResultsNR4A subfamily are involved in trophoblast differentiation, endometrial decidualization, embryo adhesion, secretion of related hormones, and regulation of spontaneous term labor. Besides, many studies have provided strong evidence that they play critical roles in spermatogenesis. Furthermore, Multiple mechanisms can affect the expression of NR4As. Broadly, NR4A family receptors affect the human reproductive system in multiple ways.ConclusionsFurther research is needed to specifically dissect the functions and regulatory mechanisms of these receptors and their pharmaceutical antagonists and agonists. The connection between the NR4A subfamily and a variety of reproductive disorders needs to be proven experimentally such that further examination of human tissue is required to assess the role of these receptors in human reproductive diseases.
Beyond the (family) binary: Family environment heterogeneity among transgender adults
Authors: Allen Samuel H. , Fish Jessica N. , McGuire Jenifer K. , Leslie Leigh A. 
Published: April 2022

AbstractObjectiveTo identify latent profiles of family environments among transgender adults and to examine the relationships between these profiles and mental and physical health.BackgroundHow a family‐of‐origin responds to a transgender family member has significant implications for that individual's mental and physical health. However, the extant research on the health of transgender people has largely relied on a binary conceptualization of family environments that has been critiqued as simplistic, reductionistic, and misrepresentative. To date, no known quantitative studies have assessed transgender family environments beyond this ostensibly antiquated conceptualization.MethodData were collected in 2018 from transgender adults via Amazon's MTurk (N = 873). Participants completed questionnaires about their family environment, experiences of minority stress, mental and physical health, and quality of life.ResultsLatent profile analysis yielded five profiles of transgender family environments: disengaged; accepting and affirming; repudiating; moderate family ambiguity; and high family ambiguity. Over half of respondents were assigned membership to one of the two ambiguity profiles (56.4%). Distal regression models demonstrated that, relative to embracing and affirming, membership in each of the other four profiles was negatively associated with both mental and physical health. Participants assigned to the ambiguity profiles did not statistically differ on health outcomes when compared to those in the repudiating profile.ConclusionAmbiguity is prevalent in transgender people's families‐of‐origin. It also appears equally detrimental as family rejection is to the mental and physical health of transgender adults.
Parents taking action adapted to parents of Black autistic children: Pilot results
Authors: Dababnah Sarah , Kim Irang , Magaña Sandy , Zhu Yan 
Published: April 2022

AbstractThere is a dearth of culturally relevant, family‐centered programs for parents raising Black autistic children, despite significant racial disparities in autism diagnoses and service access. Using a mixed‐methods convergent study design, we report qualitative and quantitative findings from a pilot trial of a peer‐to‐peer program, Parents Taking Action (PTA), adapted to primary family caregivers of Black autistic children with or at increased likelihood for autism. We aimed to understand the feasibility of delivering PTA with fidelity to the manual; participants' acceptability of program strategies; and pre–post changes in child, parent, and family outcomes. Three peer mentors (“Parent Leaders”) delivered the 14‐session intervention to seven participants. All Parent Leaders and participants were mothers of Black autistic children. We interviewed participants and Parent Leaders regarding program content and outcomes. We also collected fidelity checklists and pre–post, parent‐report measures on autism knowledge, family outcomes (e.g., advocacy), stress, depression, and child behavior. Parents reported on program acceptability at post‐intervention only. We found the Parent Leaders delivered the program with overall high levels of fidelity, based on our observations and Parent Leaders' self‐reported checklists. Participants reported high acceptability of the intervention strategies and provided feedback on methods to improve application of the content. We found statistically significant pre–post quantitative improvements in parent‐reported knowledge of their rights and child advocacy, autism knowledge, and child social competence. Participants and Parent Leaders expanded on these changes in interviews, and identified improvements in autism knowledge, parent stress and depression, family outcomes, and child behavior related to program participation. We conclude PTA is a promising, culturally and contextually relevant intervention for parents of Black autistic children. A randomized controlled trial is needed to rigorously evaluate changes in child, parent, and family outcomes.
Formation of fused aggregates under long‐term microgravity conditions aboard the ISS with implications for early solar system particle aggregation
Authors: Koch Tamara E. , Spahr Dominik , Tkalcec Beverley J. , Christ Oliver , Genzel Philomena‐Theresa , Lindner Miles , Merges David , Wilde Fabian , Winkler Björn , Brenker Frank E. 
Published: April 2022

AbstractIn order to gain further insights into early solar system aggregation processes, we carried out an experiment on board the International Space Station, which allowed us to study the behavior of dust particles exposed to electric arc discharges under long‐term microgravity. The experiment led to the formation of robust, elongated, fluffy aggregates, which were studied by scanning electron microscopy, electron backscatter diffraction, and synchrotron micro‐computed tomography. The morphologies of these aggregates strongly resemble the typical shapes of fractal fluffy‐type calcium‐aluminum‐rich inclusions (CAIs). We conclude that a small amount of melting could have supplied the required stability for such fractal structures to have survived transportation and aggregation to and compaction within planetesimals. Other aggregates produced in our experiment have a massy morphology and contain relict grains, likely resulting from the collision of grains with different degrees of melting, also observed in some natural CAIs. Some particles are surrounded by igneous rims, which remind in thickness and crystal orientation of Wark–Lovering rims; another aggregate shows similarities to disk‐shaped CAIs. These results imply that a (flash‐)heating event with subsequent aggregation could have been involved in the formation of different morphological CAI characteristics.
Functional differentiation and complementation of alkaline phosphatases and choreography of DOP scavenging in a marine diatom
Authors: Zhang Kaidian , Li Jiashun , Wang Jierui , Lin Xin , Li Ling , You Yanchun , Wu Xiaomei , Zhou Zhi , Lin Senjie 
Published: April 2022

AbstractFacing phosphate deficiency, phytoplankton use alkaline phosphatase (AP) to scavenge dissolved organophosphate (DOP). AP is a multitype (e.g., PhoA, PhoD) family of hydrolases and is known as a promiscuous enzyme with broad DOP substrate compatibility. Yet, whether the multiple types differentiate on substrates and collaborate to provide physiological flexibility remain elusive. Here we identify PhoA and PhoDs and document the functional differentiation between PhoA and a PhoD (PhoD_45757) in Phaeodactylum tricornutum. CRISPR/Cas9‐based mutations and physiological analyses reveal that (1) PhoA is a secreted enzyme and contributes the majority of total AP activity whereas PhoD_45757 is intracellular and contributes a minor fraction of the total AP activity, (2) AP gene expression compensates for each other after one is disrupted, (3) the DOP→PhoA→phosphate_uptake and the DOP_uptake→PhoD→phosphate pathways function interchangeably for some DOP substrates. These findings shed light on the underpinning of AP’s multiformity and have important implications in phytoplankton phosphorus‐nutrient niche differentiation, physiological plasticity, and competitive strategy.
A Comparison Between Retaining and Resecting the Posterior Longitudinal Ligament in Percutaneous Endoscopic Transforaminal Discectomy for Disc Herniation: A Retrospective Cohort Study
Authors: Hu Wenhao , Hu Fanqi , Liu Chao , Liu Weibo , Jiang Yi , Li Jing , Wang Yan , Li Teng , Li Li , Zhang Xuesong 
Published: April 2022

ObjectiveTo compare the efficacy and safety of retaining the posterior longitudinal ligament (PLL) with resecting the PLL in inside‐out percutaneous endoscopic transforaminal discectomy (PETD) surgery for lumbar disc herniation (LDH).MethodA total of 135 patients with symptomatic LDH who were treated by inside‐out PETD surgery from January 2015 to January 2017were included in this retrospective analysis. There were 38 males and 30 females in the PLL resection group (mean age = 52.40 ± 8.73 years) and 35 males and 32 females in the PLL retention group (mean age = 53.50 ± 9.24 years). The visual analogue scale (VAS) score, Oswestry disability index (ODI), and modified MacNab criteria were used to evaluate clinical outcomes. Operation time, blood loss, recurrence of LDH, and complications were recorded. Three months after surgery, magnetic resonance imaging was performed to confirm that nerve root compression was relieved.ResultsThe VAS and ODI scores improved significantly immediately after surgery, at 1 month after surgery, at 3 months after surgery, and at last follow‐up compared with those before surgery (P < 0.01). The scores also improved significantly between immediately after surgery and 1 month after surgery in the two groups (P < 0.001). Multivariate analysis indicated that age ≥ 50 years (odds ratio (OR) = 6.33, 95% confidence interval (CI): 1.64–21.98, P = 0.014), pain duration ≥6 months (OR = 4.68, 95% CI: 1.29–6.51, P = 0.025), pre‐ODI score ≥ 40% (OR = 5.97, 95% CI: 2.41–14.86, P = 0.003) were all associated with poor functional outcomes. There was no significant difference in the excellent/good ratio between the two groups and the mean operation time of the retention group was 71.5% of that of the resection group (82.7 ± 18.5 min vs 115.6 ± 24.6 min, P < 0.01). In the patients, no serious complications, such as dural tear, wound infection, or persistent nerve root injury, were observed during the follow‐up period. There was no significant difference in the complication rate between the two groups (6/68 vs 6/67, P = 0.979). Although the recurrence rate was higher in retention group, there was no significant difference between the two groups (1/68 vs 2/67, P = 0.551).ConclusionThe PLL is recommended to be retained for inside‐out PETD surgery.
Full‐Endoscopic Anterior Odontoid Screw Fixation: A Novel Surgical Technique
Authors: Kotheeranurak Vit , Pholprajug Phattareeya , Jitpakdee Khanathip , Pruttikul Pritsanai , Chitragran Roongrath , Singhatanadgige Weerasak , Limthongkul Worawat , Yingsakmongkol Wicharn , Kim Jin‐Sung 
Published: April 2022

ObjectiveFirst, to propose a novel minimally invasive technique of full‐endoscopic anterior odontoid fixation (FEAOF) that aims to reduce the risk of retropharyngeal approach (both open and percutaneous techniques) to anterior odontoid screw fixation. Second, to describe steps of the procedure and, lastly, to report the initial outcomes in patients treated with this novel technique.MethodsFour non‐consecutive patients who were diagnosed with a displaced odontoid fracture (Anderson‐D'Alonzo classification type II and Grauer subclassification type A or B) from 2019 to 2020 underwent surgical fixation by our novel technique for anterior odontoid screw fixation. A detailed technical approach of FEAOF for the surgical treatment of type II odontoid fractures was described, and the patients' outcomes based on postoperative radiographic results including computed tomography (CT), clinical outcome parameters including visual analogue scale (VAS) for neck pain both preoperatively and at postoperative follow‐up, and range of neck motion at the final follow‐up were reported.ResultsThe mean age was 33.5 years (24–41), three patients were male. The mean operative time was 93.75 min, and the mean blood loss was 7.5 ml. An immediate post‐operative thin‐sliced CT showed that all patients achieved satisfactory reduction and proper screw position. No screw malposition or penetration was found. At a 6‐month follow‐up, a thin‐sliced CT demonstrated solid bony union in every case. The mean VAS for neck pain was reduced from 6.5 to 0.6 at the 6‐months follow‐up. At the final follow‐up, all patients showed improvement in ranges of motion without any complications; however, one patient was lost to follow‐up.ConclusionsFEAOF is a feasible and effective option for treating type II odontoid fractures. The procedure is less invasive than other techniques and provides clear direct visualization of the involved structures.
Change of Pelvic Sagittal Tilt after Total Hip Arthroplasty in Patients with Bilateral Crowe Type IV Developmental Dysplasia of the Hip
Authors: Zhang Bohan , Du Yinqiao , Sun Jingyang , Shen Junmin , Li Tiejian , Zhou Yonggang 
Published: April 2022

ObjectiveTo explore and analyze the change of pelvic sagittal tilt (PST) after total hip arthroplasty (THA) in patients with bilateral Crowe type IV developmental dysplasia of the hip (DDH).MethodsThe study retrospectively evaluated 43 patients with bilateral Crowe type IV DDH undergoing THA from January 2008 to June 2019 who were followed up for 12 months postoperatively. Four parameters, including the ratio between the height and width of the obturator foramina(H/W ratio), the vertical distance between the upper edge of the symphysis and the middle of the sacrococcygeal joint (SSc distance), the vertical distance between the upper edge of the symphysis and the line connecting bilateral hip centers (SC distance) and the vertical distance between the upper edge of the symphysis and the line connecting the bilateral lower ends of the sacroiliac joints (SSi distance), which could indirectly reflect the change of PST, were observed and measured by radiographs. The change of each parameter before operation, immediately after operation, and in 3, 6 and 12 months postoperatively was compared and analyzed.ResultsCompared with the value before operation, the H/W ratio immediately after operation and in 3, 6 and 12 months postoperatively were 0.61 ± 0.12 (t = 0.893, P = 0.377), 0.61 ± 0.11 (t = 1.622, P = 0.112), 0.67 ± 0.10 (t = 5.995, P < 0.001) and 0.76 ± 0.12 (t = −9.313, P < 0.001), respectively, and the SSc, SC and SSi distance in 6 months postoperatively were 30.12 ± 7.06 mm (t = 3.506, P = 0.002), 42.8 ± 7.7 mm (t = 5.843, P < 0.001), 129.3 ± 12.6 mm (t = 5.888, P < 0.001), respectively, and in 12 months postoperatively were 27.24 ± 7.68 mm (t = 6.510, P < 0.001), 36.1 ± 9.1 mm (t = 9.230, P < 0.001), 118.9 ± 14.9 mm (t = 8.940, P < 0.001), respectively. The radiographs obtained in 6 and 12 months postoperatively demonstrated a significantly increased H/W ratio and decreased SSc, SC and SSi distance. At the last follow‐up, the clinical evaluations significantly improved in all patients and there were no revisions.ConclusionThe significant change of pelvic sagittal posterior tilt in patients with bilateral Crowe type IV DDH might be a significant phenomenon after THA, which could occur in 6 months postoperatively.