bluuebunny/crossref_metadata_2025_split huggingface dataset:
First assessment of Polycyclic Aromatic Hydrocarbons contamination and associated human health risk in Mullet (Liza aurata) from Tunisia: case of Bizerte and Ghar El Melh Lagoons
Authors: Ameur Walid Ben , Annabi Ali , Mhadhbi Takoua , Hassine Sihem Ben , Safouen Ghanmi , Megdiche Yassine El , Khadija Maaned , Ennaceur Soukaina , Trabelsi Souad , Hammami Bechir , Touil Soufiane , Driss Mohamed Ridha 
Published: May 2022

Abstract
Data about the presence of polycyclic aromatic hydrocarbons in biota from Tunisian coastal ecosystems is scarce. To our knowledge, this is the first work to report these chemicals in the biota of Ghar El Melh Lagoon. In Liza aurata muscle from Bizerte and Ghar El Melh Lagoons (Tunisia), levels of 15 priority polycyclic aromatic hydrocarbons were measured.Total PAH levels in mullet from Bizerte and Ghar El Melh Lagoons were comparable to or lower than those found in other fish specimens from around the globe. The nature of the detected chemicals reveals petrogenic and pyrolytic anthropogenic contamination in both studied lagoons.Threat to human health caused by mullet consumption was assessed and it was observed that PAH intakes don’t threat to local population's health.
Osteopontin promotes cell pelletizing and angiogenesis in triple negative breast cancer by activating the PI3k/Akt/mTOR pathway to regulate GPX4-mediated anti-lipid peroxidation levels
Authors: Guo Man , Li Weihan , Liu Mengyue , Wang Cao , Zhang Lu , Zhang Hao 
Published: May 2022

Abstract
Background
Triple negative breast cancer (TNBC) features high aggressiveness, metastasis rate, drug resistance as well as poor prognosis. Osteopontin (OPN) is a key protein in the process of osteogenesis and has been found to be a new tumor marker in recent years. This study will discuss the regulatory role that OPN plays in TNBC as well as its unconcealed mechanism.
Methods
The cell viability was tested utilizing CCK-8 while Transwell as well as wound healing was adopted to test cell invasive and migrative capabilities. The pellet size of each group was detected by tumor-sphere formation assay. HUVEC tube formation assay was used to measure the ability of tumor cells to form blood vessels. Western blot was applied for the estimation of globule-forming-, angiogenesis-, PI3k/Akt/mTOR signaling pathway-related proteins as well as OPN. The levels of oxidative stress-related markers were assessed with the help of ELISA. Through transfection, OPN in MD-MB-436 cells was overexpressed and PI3k/Akt/mTOR pathway inhibitor LY294002 was employed for the administration of cells. After that, a series of functional experiments were implemented to investigate the mechanism of tumor cells. Finally, in vitro experiment of tumor-bearing mice were performed to verify.
Results
The proliferative, invasive and migrative capabilities as well as angiogenesis of TNBC cells were conspicuously ascended in contrast with non-TNBC cell lines. OPN expression in TNBC cells was dramatically enhanced. OPN upregulation rapidly elevated cell proliferative, invasive and migrative capabilities as well as angiogenesis, which may be achieved by activating PI3k/Akt/mTOR to regulate GPXX4-mediated anti-lipid peroxidation.
Conclusion
OPN can promote cell pelletizing and angiogenesis in TNBC by activating the PI3k/Akt/mTOR pathway to regulate GPX4-mediated anti-lipid peroxidation levels.
Myocyte culture with decellularized skeletal muscle sheet with observable interaction with extracellular matrix
Authors: Nakada Satoshi , Yamashita Yuri , Akiba Seiya , Shima Takeru , Arikawa-Hirasawa Eri 
Published: May 2022

Abstract
Background: In skeletal muscle, muscle fibers are highly organized and bundled within the basement membrane. Several microfabricated substrate models have failed to mimic the macrostructure of native muscle, including various extracellular matrix (ECM) proteins. Even in substrates coated with proteins consisting of skeletal muscle-specific ECM compositions extracted from skeletal muscle, the function of each ECM protein remains, but the structural features of the basement membrane are lost. Therefore, in this study, we used decellularized muscle tissue and mouse myoblasts C2C12. We developed and evaluated a system to analyze the interaction between native ECM and myocytes. Methods: Chicken skeletal muscle was sliced into sheets and decellularized with 1% SDS solution, 0.1N NaOH solution, and 50% ethanol to prepare decellularized skeletal muscle sheets (DSMS). C2C12 was then seeded and differentiated on DSMS. Immunostaining for ECM molecules was performed to examine the relationship between myoblast adhesion status, myotube orientation, and collagen IV orientation. Survival of myotubes in long-term culture was also confirmed by calcein staining. Results: Decellularization of sliced skeletal muscle created a flexible skeletal muscle ECM-derived culture substrate. C2C12 myoblasts adhered to scaffolds in the DSMS and developed adhesion plaques and filopodia. In addition, C2C12 myotubes showed orientation along with the ECM orientation within the DSMS. Furthermore, RT-PCR results showed that C2C12 myogenic differentiation was promoted on the DSMS since the expression levels of myoD, etc., were increased early. On the plastic dish, detachment of cells occurred from day 6 of differentiation, and few cells survived to day 12, but on DSMS, no detachment occurred even on day 12, allowing long-term culture.Conclusions: This study reports a novel myocyte culture method using sheet-like ECM structures obtained by decellularization. Myoblasts adhered to the sheet according to the orientation of the ECM, and the direction of myotube formation could be controlled. This culture technique reproduces a cell culture environment that reflects the properties of living skeletal muscle and allows studies on the interaction between the ECM and myocytes.
Utilization and compliance with iron supplementation and predictors among pregnant women in Southeast Ethiopia
Authors: Beressa Girma , Lencha Bikila , Bosha Tafese , Egata Gudina 
Published: May 2022

Abstract
Globally, anemia affects 1.62 billion people (25%) of which 56 million are pregnant women. However, evidence for utilization and compliance with iron supplementation and predictors during pregnancy, in low-income countries, including Ethiopia, was sparse and inconclusive. Therefore, this study aimed to assess utilization and compliance with iron supplementation and predictors among pregnant women in Robe Town, Southeast Ethiopia. A community-based cross-sectional study was employed among randomly selected 445 pregnant women attending antenatal care at health facilities from May to July 2015. Systematic random sampling was used to select respondents. Data were collected using a pre-tested interviewer-administered, structured questionnaire. Multivariable logistic regression analyses were used to identify predictors of compliance with iron supplementation. Odds ratio, along with a 95% confidence interval (CI) was used to estimate the strength of the association. In this study, 54%; 95% CI: (49.4, 58.4%), 45.2%; 95% CI: (40.9, 49.4%), 4.3%; 95% CI: (2.5, 6.3%), and 2.2%; 95% CI: (1.1, 3.6%) of women received iron supplements during their first, second, third, and fourth antenatal care visits, respectively. The level of compliance with iron supplementation was 92.4%; 95% CI: (89.9, 94.6%). Having a formal education (AOR=4.45, 95% CI: 1.41, 13.99), high wealth quintile (AOR = 0.18, 95% CI: 0.05, 0.68), medium wealth quintile [(AOR=0.33, 95% CI: (0.11, 0.98)], iron supplements for free (AOR=3.77, 95% CI: 1.33, 10.69), not experiencing discomfort related to iron supplements intake (AOR= 2.94, 95% CI: 1.17, 7.39), having comprehensive knowledge about anemia (AOR=2.62, 95% CI: 1.02, 6.70), being knowledgeable about iron supplements (AOR=3.30, 95% CI: 1.12, 9.76), having information about importance of iron supplementation during pregnancy (AOR=2.86; 95% CI: 1.04, 7.87), and ever being visited by urban health extension workers (AOR=0.31; 95% CI: 0.12, 0.83) was significantly associated with compliance with iron supplementation during pregnancy. The utilization of iron supplementation during pregnancy was low, with relatively high compliance with the supplements. Thus, comprehensive nutrition education and free provision of iron supplementation are crucial tools to increase utilization and compliance with iron supplementation during pregnancy. Further research with a strong study design using golden standard methods is warranted.
Synthesis and microwave absorption properties of Ni0.5Zn0.5Fe2O4/CI composite coated with polyaniline within paraffin wax matrix
Authors: houbi anas , Aldashevich Zharmenov , Atassi Yomen , Telmanovna Zhenisgul , Saule MIRZALIEVA 
Published: May 2022

Abstract
In this paper, Ternary composites of polyaniline/NiZn ferrite/carbonyl iron (PANI/F/CI) are prepared via two stages: Firstly, Ni0.5Zn0.5Fe2O4 is prepared using a sol-gel method while carbonyl iron is commercially purchased. After that, PANI/F/CI composites are prepared using an in-situ polymerization technique of PANI in the existence of the Ni0.5Zn0.5Fe2O4 and CI. X-ray diffractometry, FTIR spectroscopy, UV-vis spectroscopy, TGA analysis, and SEM analysis are utilized in order to characterize samples. The morphology of the powders is investigated by SEM. The electromagnetic interference shielding and microwave absorption properties are measured in the frequency band of 8.8–12 GHz to investigate the microwave characterization. The results refer those microwave absorption properties are related to the absorber thickness and the loading ratio of the absorber within a paraffin matrix. Minimal reflection loss of -30.8 dB at the matching frequency of 10.3 GHz and the absorption bandwidth under − 10 dB of 2.8 GHz for 3.4 mm thickness with a surface density of 3.38 kg/m2 are noticed for the PANI/F/CI composite sample. The maximum shielding efficiency of 30.12 dB at 11.0 GHz for 3.2 mm thickness is observed for the PANI/F/CI composite sample.
Research on MEEMD Decomposition-Prediction-Reconstruction Prediction Model of Summer Precipitation Sequence in Wujiang River Basin
Authors: Wang Yongtao , Liu Jian , Li Rong , Suo Xinyu , Lu EnHui 
Published: May 2022

Abstract
Aiming at the low prediction accuracy of precipitation time series data, proposes an Modified Ensemble Empirical Mode Decomposition-Prediction-Reconstruction Model（MDPRM）. First,The non-stationary precipitation time series is decomposed into multiple decomposition terms by MEEMD. Then, according to the characteristics of different decomposition items,the particle swarm optimization support vector machine (PSO-SVM), convolutional neural network (CNN) and recurrent neural network (RNN) models are used for prediction respectively. Finally, the prediction results of each decomposition item are superimposed and reconstructed to form the final prediction results. The summer precipitation in the Wujiang River Basin in Guizhou Province from 1961 to 2018 was used as an example to verify the results. The results show that:compared with BP, SVM, RNN and other prediction methods, the MDPRM proposed in this paper has higher prediction accuracy, and can effectively guide the regional water resources allocation and scheduling, drought relief and disaster reduction.
Designing legislative responses to restrict children’s exposure to unhealthy food and non-alcoholic beverage marketing: a case study analysis of Chile, Canada and the United Kingdom
Authors: Sing Fiona , Reeve Belinda , Backholer Kathryn , Mackay Sally , Swinburn Boyd 
Published: May 2022

Abstract
Introduction:
Introducing legislation that restricts companies from exposing children to marketing of unhealthy food and beverage products is both politically and technically difficult. To advance the literature on the technical design of food marketing legislation, and to support governments around the world with legislative development, we aimed to describe the legislative approach from three governments.
Methods
A multiple case study methodology was adopted to describe how three governments approached designing comprehensive food marketing legislation (Chile, Canada and the United Kingdom). A theoretical framework outlining best practice design principles guided our methodological approach to examine how each country designed the technical aspects of their regulatory response, including the regulatory form adopted, the substantive content of the laws, and the implementation and governance mechanisms used. Data from documentary evidence and 15 semi-structured key informant interviews were collected and synthesised using a directed content analysis.
Results
All three countries varied in their legislative design and were therefore considered of variable strength regarding the legislative elements used to protect children from unhealthy food marketing. While some elements of best practice design were present, particularly relating to the governance of legislative design and implementation, the scope of each law (or proposed laws) had limitations. These included: the exclusion of brand marketing; not protecting children up to age 18; focusing solely on child-directed marketing instead of all marketing that children are exposed to; and not allocating sufficient resources to effectively monitor and enforce the laws. The United Kingdom’s approach to legislation is the most comprehensive and more likely to meet its regulatory objectives.
Conclusions
Our synthesis and analysis of the technical elements of food marketing laws can support governments around the world as they develop their own food marketing restrictions. An analysis of the three approaches illustrates an evolution in the design of food marketing laws over time, as well as the design strengths offered by a legislative approach. Opportunities remain for strengthening legislative responses to protect children from unhealthy food marketing practices.
A Retrospective Single-center Analysis of Prenatal Diagnosis and Follow-up of 626 Chinese Patients with Positive Non-invasive Prenatal Testing Results
Authors: Bu Xiufen , Zhou Shihao , Li Xu , Li Shihong , Li Hongyu , Ding Siyi , He Jun , Linpeng Siyuan 
Published: May 2022

Abstract
Background: This study explores the diagnostic efficiency of different prenatal diagnostic approaches for women with positive non-invasive prenatal testing (NIPT) results by analyzing their clinical information and pregnancy outcome. Methods: We collected data on 626 NIPT-positive pregnant women from January 2017 to June 2021 and arranged subsequent prenatal diagnostic operations for them after genetic counseling, along with long-term intensive follow-up. A total of 567 women accepted invasive prenatal diagnosis (IPD) (90.57%), and 262 cases were confirmed as true positives of NIPT. Results: The positive predictive value for trisomy 21 (T21), trisomy 18 (T18), trisomy 13 (T13), sex chromosome aneuploidies (SCAs), rare autosomal trisomies (RATs), and microdeletion and microduplication syndromes (MMS) were 81.13%, 37.93%, 18.42%, 48.83%, 18.37%, and 41.67%, respectively. Discordant results between NIPT and IPD were seen in 53 cases, with the discordance rate being 9.35%. Additionally, there were 43 cases with discordant results between karyotyping and chromosomal microarray analysis (CMA)/copy number variation sequencing. Conclusions: Additional reporting of RATs and MMS with routine NIPT that only detects T21/T18/T13 and SCAs can yield diagnosis more accurately. However, NIPT cannot be used as a substitute for IPD owing to its high false positive rate and discordances with other diagnostic methods. We recommend CMA combined with karyotyping as the preferred method for accurate diagnosis of NIPT-positive women.
Rational solutions with zero background and algebraic solitons of three derivative nonlinear Schrödinger equations
Authors: Wang Jing , Wu Hua 
Published: May 2022

Abstract
In this paper we provide a systematical bilinear approach to derive rational solutions (in terms of envelope | q |) living on a zero background and decaying algebraically for three derivative nonlinear Schrödinger equations, namely, the Kaup-Newell equation, the Chen-Lee-Liu equation and the Gerdjikov-Ivanov equation. We present a simpler unified bilinear form for these three equations. Their rational solutions with zero background are obtained in terms of double Wronskian via bilinear equations. Algebraic solitons resulting from rational solutions are presented. Asymptotic dynamics are analyzed and illustrated. Scattering of high order rational solutions are featured as waves with slowly varying amplitudes. Scattering of algebraic solitons behaves like usual solitons but asymptotically with zero phase shift.PACS numbers: 02.30.Ik, 02.30.Ks, 05.45.Yv
Factors inﬂuencing ellipsoid zone tear in pathologic myopia choroidal neovascularization
Authors: Jing Ruixia , Chen Defang , Li Xue , Wang Zhen 
Published: May 2022

Abstract
This study explored factors influencing ellipsoid zone (EZ) tear in pathologic myopia choroidal neovascularization (PM-CNV) and its relationship with visual acuity mainly using optical coherence tomography (OCT). We divided 21 (22 eyes) PM-CNV patients, who were divided into non-tear (n=10) and tear (n=12) groups by whether the EZ was torn or not. Measured central macular thickness (CMT), PM-CNV lesion hyperreflectivity (HF) height and area, and myoid ellipsoid zone (MEZ)) thickness on OCT. Analyzing relationships between these factors and EZ tearing. We found EZ tear group had thicker CMT (P = 0.006), higher HF height (P = 0.001), larger HF area (P = 0.001), bigger PM-CNV area (P = 0.028), greater HF/CNV area ratio (P = 0.038), thinner MEZ (P = 0.000), worse best corrected visual acuity (BCVA) (P = 0.000). After treatment with conbercept only 4/12 eyes showed recovery of EZ integrity in tear group, with even worse BCVA. OCT provides a new bioindicator as monitoring risk factor of EZ tear for PM-CNV. PM-CNV with higher and larger HF, bigger HF area relative to the PM-CNV area have greater risk for EZ tear and poorer visual acuity. Better visual acuity will be achieved when PM-CNV was treated before EZ tear.
Chronic pain, mental health and functional impairment in adult refugees from Syria resettled in Norway: a cross-sectional study
Authors: Nissen Alexander , Hynek Kamila Angelika , Scales David , Hilden Per Kristian , Straiton Melanie 
Published: May 2022

Abstract
Background: Limited research exists on pain and especially the co-occurrence of pain and mental ill health in general refugee populations. The present study aimed to assess the prevalence of chronic pain (CP) among adult refugees from Syria resettled in Norway; investigate the association between CP and mental ill health; and explore how CP and mental ill health associate with both perceived general health and functional impairment. Gender as potential effect modifier in these associations was also examined.Methods: Cross-sectional, postal survey questionnaire. Inclusion criteria: ≥18 years old; refugee from Syria; and arrived in Norway between 2015 and 2017. Study sample was randomly drawn from full population registries, and n=902 participated (participation rate ≈10%). CP measured with 10 items on pain lasting for ≥3 consecutive months last year. Symptoms of anxiety, depression and PTSD were measured with the HSCL and HTQ scales, respectively. Ordered and binomial logistic regressions were used in analyses. Gender was tested as effect modifier with Wald test for interaction.Results: The overall prevalence of severe CP was 43.1%. There was strong evidence that anxiety, depression and PTSD were associated with higher levels of CP. In fully adjusted regression models, including both CP and mental health variables, CP was strongly associated with poor perceived general health whereas mental health showed much weaker associations. The association between mental health (anxiety and depression) and functional impairment was highly gender specific, with strong associations in men but not in women. CP was strongly associated with functional impairment with no difference across gender. Conclusion: The study shows a high burden of CP in a general population of adult refugees from Syria with likely substantial adverse consequences for daily functioning. The strong association between CP and mental ill health suggests personnel working with refugees’ health should be attuned to their co-occurrence as both problems may need to be addressed for either to be effectively mitigated. A clear mismatch exists between the burden on health caused by pain in general refugee populations and the amount of available evidence to guide mitigating strategies. Trial registration: NCT03742128
Methane hydrate nuclei in dynamic network of hydrogen bonds of supersaturated solution outside the region of hydrate stability: molecular dynamics study
Authors: Belosludov Vladimir R. , Gets Kirill V. , Zhdanov Ravil K. , Bozhko Yulia Yu. , Belosludov Rodion V. , Chen Li-Jen , Kawazoe Yoshiyuki 
Published: May 2022

Abstract
Using molecular dynamics the evolution of metastable solution "methane + water" was studied at various methane concentrations at T = 270 K and P = 1 bar. We have shown that the process of hydrate formation is a collective process of the H-bond network reorganization in solution at the initial stage (0–1 ns) until the first fluctuating cavities appear. This is associated with preceding increase of tetrahedrality of each water molecule local order. The molecules with the high degree of tetrahedrality are distributed in volume uniformly. The formation of small and large fluctuation of sI cavities is shown before the steady hydrate growth begins. Later, the constantly presented hydrate cavities become the basis for few independent hydrate nucleation centers.