bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiology of Extrapulmonary Tuberculosis in Brunei Darussalam: A retrospective cohort study
Authors: Chaw Liling , Salleh Lena Mat , Hamid Rafizah Abdul , Thu Kyaw 
Published: November 2022

AbstractObjectivesWe reported the incidence and associated factors of extrapulmonary tuberculosis (EPTB).DesignA retrospective cohort study.SettingBrunei Darussalam, an intermediate tuberculosis (TB)-burden country with stagnating annual TB rates.ParticipantsAll active TB cases identified in the country between January 2001 and December 2018 (18 years)Primary and secondary outcome measuresAnnual proportions of EPTB (overall and specific) were calculated. Multiple logistic regression was done to investigate factors associated with developing EPTB, when compared to pulmonary TB (PTB). Chi-square trend test was used to determine any trends during the 18-year study period.ResultsWe identified 3,916 TB cases, among which 743 (19.0%) were EPTB cases. Lymphatic (44.8%) and pleural (19.4%) EPTB were most common. The main modes of diagnosis were tissue biopsy (73.6%) and radiologic assessment (18.3%). Treatment success and mortality rate were 79.7% and 7.0%, respectively. Associations with specific EPTB types varies with age-group and gender. Younger age-group (adjusted Odds Ratio [aOR] ≥1.94) and females (aOR: 2.45 [95%CI: 1.94,3.11]) had higher adjusted odds of developing lymphatic EPTB, but had lower adjusted odds of developing pleural EPTB [younger age-group (aOR ≤0.54) and female (aOR: 0.41 [95%CI: 0.17,0.90])]. When compared to foreign residents, locals had higher adjusted odds of skeletal (aOR: 4.44 [95%CI: 2.04,11.69]), gastrointestinal (aOR: 3.91 [95%CI: 1.84,9.66]) and other types of EPTB (aOR: 3.42 [95%CI: 1.53,9.14]). No significant trend differences were observed for overall and specific EPTB types.ConclusionDespite being generally non-infectious and less recognised than PTB, understanding EPTB epidemiology is important as it also contributes to the overall TB burden in a country. Examining EPTB cases by their specific anatomical site would provide more information on risk factors. Raising public awareness on the EPTB symptoms and that TB affects lungs and other parts of the body could promote early health seeking behaviour and early EPTB diagnosis.Strengths and limitations of this study- We analysed TB case notification data, retrospectively collected as part of the national TB surveillance programme.- The main study strength is the length of the study period (18 years) and that all notified TB cases in the country were captured in this dataset.- Study limitations include the inability to distinguish between PTB cases with only lung involvement and those with miliary TB and/or concurrent PTB and EPTB, and also the inability to include data on co-morbidities.
Massively parallel single-cell sequencing of genetic loci in diverse microbial populations
Authors: Lan Freeman , Saba Jason , Ross Tyler D , Zhou Zhichao , Krauska Katie , Anantharaman Karthik , Landick Robert , Venturelli Ophelia 
Published: November 2022

ABSTRACTSingle cell genetic heterogeneity is ubiquitous in microbial populations and an important aspect of microbial biology. However, we lack a broadly applicable and accessible method to study this heterogeneity at the single cell level. Here, we introduce a simple, robust, and generalizable platform for quantitative and massively parallel single cell sequencing of target genetic loci in microbes using ultrahigh-throughput droplet microfluidics (Droplet Targeted Amplicon Sequencing or DoTA-seq). Using DoTA-seq, we elucidate the highly diverse single cell ON/OFF states of the phase-variable capsule synthesis operons in the prevalent human gut speciesBacteroides fragilis. In addition, we quantify the shifts in antibiotic resistance gene abundances in different species in a 25 member human gut microbial community in response to antibiotics. By sequencing tens of thousands of single-cells derived from a human fecal sample, we identify links between plasmid replicons and the taxonomic lineages of their associated hosts. In sum, DoTA-seq is an accessible and broadly applicable tool for profiling single-cell genetic variation in microbiomes.
Precision super-resolution cryo-correlative light and electron microscopy for rapidin situstructural analyses of optogenetically-positioned organelles
Authors: Redpath G.M.I. , Rae J. , Yao Y. , Ruan J. , Cagigas M.L. , Whan R. , Hardeman E.C. , Gunning P.W. , Ananthanarayanan V. , Parton R.G. , Ariotti N.A. 
Published: November 2022

AbstractUnambiguous targeting of cellular structures forin situcryo-electron microscopy in the heterogeneous, dense, and compacted environment of the cytoplasm remains challenging. Here we have developed a novel cryogenic correlative light and electron microscopy (cryo-CLEM) workflow which combines thin cells grown on a mechanically defined substratum to rapidly analyse organelles and macromolecular complexes in the cell by cryo-electron tomography (cryo-ET). We coupled these advancements with optogenetics to redistribute perinuclear-localised organelles to the cell periphery for cryo-ET. This reliable and robust workflow allows for fastin situanalyses without the requirement for cryo-focused ion beam milling. We have developed a protocol where cells can be frozen, imaged by cryo-fluorescence microscopy and ready for batch cryo-ET within a day.
Increased Pyramidal and VIP Neuronal Excitability in Primary Auditory Cortex Directly Correlates with Tinnitus Behavior
Authors: Ghimire Madan , Cai Rui , Ling Lynne , Brownell Kevin A. , Hackett Troy A. , Llano Daniel A. , Caspary Donald M. 
Published: November 2022

AbstractTinnitus affects roughly 15-20% of the population while severely impacting 10% of those afflicted. Tinnitus pathology is multifactorial, generally initiated by damage to the auditory periphery, resulting in a cascade of maladaptive plastic changes at multiple levels of the central auditory neuraxis as well as limbic and non-auditory cortical centers. Using a well-established condition-suppression model of tinnitus, we measured tinnitus-related changes in the microcircuits of excitatory/inhibitory neurons onto layer 5 pyramidal neurons (PNs), as well as changes in the excitability of vasoactive intestinal peptide (VIP) neurons in primary auditory cortex (A1). Patch-clamp recordings from PNs in A1 slices showed tinnitus-related increases in spontaneous excitatory postsynaptic currents (sEPSCs) and decreases in spontaneous inhibitory postsynaptic currents (sIPSCs). Both measures were directly correlated to the rat’s behavioral evidence of tinnitus. Tinnitus-related changes in PN excitability were independent of changes in A1 excitatory or inhibitory cell numbers. VIP neurons, part of an A1 local circuit that can disinhibit layer 5 PNs, showed significant tinnitus-related increases in excitability that directly correlated with the rat’s behavioral tinnitus score. That PN and VIP changes directly correlated to tinnitus behavior, suggests an essential role in A1 tinnitus pathology. Tinnitus-related A1 changes were similar to findings in studies of neuropathic pain in somatosensory cortex suggesting a common pathology of these troublesome perceptual impairments. Improved understanding between excitatory, inhibitory and disinhibitory sensory cortical circuits can serve as a model for testing therapeutic approaches to the treatment of tinnitus and chronic pain.Key pointsIdentify tinnitus-related changes in synaptic function of specific neuronal subtypes in a reliable animal model of tinnitus.Finding show direct and indirect tinnitus-related losses of normal inhibitory function at A1 layer 5 pyramidal cells, and increased VIP excitability.Findings are similar to what has been shown for neuropathic pain suggesting that restoring normal inhibitory function at synaptic inputs onto A1 pyramidal neurons could conceptually reduce tinnitus discomfort.
A new means of energy supply driven by terahertz photons recovers related neural activity
Authors: Tan Xiaoxuan 
Published: November 2022

AbstractContinuous and efficient energy capture represents a long-sought dream of mankind. The brain is a major energy consuming organ, an adult brain accounts for about 2% of the body weight but consumes about 20% of the body’s energy. However, it is still unclear how the brain achieve efficient use of energy. Here, using nerve cells as test subjects, we found that THz photons with a specific frequency can effectively restore the reduced frequency of action potentials caused by inadequate adenosine 5’-triphosphate (ATP) supply, which has been demonstrated as a novel mode of energy supply, present photons emission at a particular frequency from the breaking of the ATP phosphate bond. This energy supply mechanism may play a key biophysical basis for explaining how the body efficiently obtain energy, because the quantized chemical reactions could have a high energy efficiency and ultrahigh selectivity compared with the traditional thermochemistry and photochemistry.
Notch engagement by Jag1 nanoscale clusters indicates a force-independent mode of activation
Authors: Smyrlaki Ioanna , Fördös Ferenc , Lago Iris Rocamonde , Wang Yang , Lentini Antonio , Luca Vincent C. , Reinius Björn , Teixeira Ana I. , Högberg Björn 
Published: November 2022

ABSTRACTThe Notch signaling pathway is a cell-cell communication system with fundamental roles in embryonic development and the nervous system. The model of Notch receptor activation that is currently most accepted, involves a force-induced conformation change at the negative regulatory region of the receptor, the subsequent recruitment of ADAM metalloproteases and a cleavage cascade that releases the Notch intracellular domain. Here, we define conditions that enable force-independent Notch activation through the formation of soluble, long-lived, multivalent ligand-receptor complexes. To investigate how ligand valency affects activation of Notch receptors, we treated iPSc-derived neuroepithelial stem-like (lt-NES) cells with different spatially defined, molecularly precise ligand nanopatterns on DNA origami nanostructures. Our data indicate that Notch signaling is activated via stimulation with multivalent clusters of the ligand Jag1, and even multivalent chimeric structures where some Jag1 proteins are replaced by other binders that do not target Notch. The findings are corroborated by systematic elimination, through experimental control, of several confounding factors that potentially could generate forces, including electrostatic interactions, endocytosis and non-specific binding. Taken together, our data suggest a model where Jag1 ligands are able to activate Notch receptors upon prolonged binding, which subsequently triggers downstream signaling in a force independent manner. These findings reveal a distinct mode of activation of Notch and could lay the foundation for the development of soluble Notch agonists that currently remain elusive.
Toward acceptance of future foods: the role of trust and perception in consumption intentions of plant-based meat alternatives
Authors: Begho Toritseju , Odeniyi Kehinde , Fadare Olusegun 
Published: November 2022

PurposeFuture foods such as plant-based meat alternatives (PBMA) are a means to achieving a more sustainable food system. However, there is a gap in what is known about PBMA from the consumer side, considering it is a relatively new food. Therefore, the paper aims to examine whether trust and perception could explain the intention to consume PBMA among Chinese adults.Design/methodology/approachThe paper elicited the level of trust and perceptions related to PBMA. Then logistic regressions and mediation analysis were estimated to determine the associations between consumption intentions towards PBMA and a range of trust and perception variables.FindingsThe results indicate that most respondents trust food safety regulators and the labelling and composition standards. A comparison of the perception of meat and PBMA revealed that the majority of respondents perceive meat as tastier than PBMA while PBMA as being better for the environment. Regarding the effect of trust and perception on consumption intention, respondents that perceive PBMA as being better for the environment and having lower food safety risks are more likely to eat PBMA. Also, consumption intentions for PBMA are higher among respondents who trust safety regulators and independent promoters.Practical implicationsThe finding on both the intention to try and the potential for sustained consumption is a prerequisite to predicting future demand. These findings are also crucial to guiding market orientation.Originality/valueThis paper focuses on drivers/barriers of consumer consumption intention – a shift from studies which examine product attributes and sensory or marketing determinants of consumption decisions.
The co-movement effect of managers' psychological factors on the BIM adoption decision in SMEs: a study based on fsQCA
Authors: Li Rui , Niu Zhanwen , Liu Chaochao , Wu Bei 
Published: November 2022

PurposeGiven the complexity of building information modeling (BIM) adoption decisions in small- and medium-sized enterprises (SMEs) in the Architecture, Engineering and Construction (AEC) industry, understanding BIM adoption decision-making through the net effect of a single factor on BIM adoption decisions alone is limited. Therefore, this paper analyzed the co-movement effect of managers' psychological factors on the BIM adoption decisions from the perspective of managers' perceptions. The purpose is to let managers have a deep understanding of their BIM adoption decisions, and put forward targeted suggestions for the AEC industry to promote the adoption of BIM by SMEs.Design/methodology/approachData from 192 managers in SMEs collected by the questionnaire were used in a fuzzy set qualitative comparative analysis (fsQCA). Due to the limitations of fsQCA in making the best use of the data used, as a complement to fsQCA, necessary conditions analysis (NCA) was used to analyze the extent to which necessary conditions influenced the outcome.Findings(1) NCA analysis shows that high perceived resource availability (PRA) and high performance expectancy (PE) are necessary conditions for high BIM adoption intention (AI). (2) fsQCA analysis shows that high PE is the single core condition for high AI. fsQCA analysis identifies three configurations of managers' psychological factors, reflecting three types of managers' decision preferences, namely benefit preference, loss aversion and risk avoidance, respectively. Different decision preferences may lead to different BIM adoption strategies, such as full in-house use, partial in-house/outsourcing and full outsourcing of BIM processes. (3) High perceived risk (PR) and low perceived business value of BIM (PBV) are the core conditions for low AI.Originality/valueThis paper expands on the application of fsQCA to context of BIM adoption decisions. Based on the results of fsQCA analysis, this paper also establishes the relationship between managers' decision-making psychology and BIM adoption strategy choice and analyzes the impact of different decision biases on BIM adoption strategy choice. It concludes with suggestions for encouraging managers to adopt BIM and for avoiding decision-making bias.
Toward an integrated model for the antecedents and consequences of AIS usage at the organizational level
Authors: Al-Okaily Manaf 
Published: November 2022

PurposeThe purpose of this study is to test the antecedent factors that directly influence accounting information systems (AIS) usage, which, in turn, affects net benefits of AIS usage, drawing upon the technology acceptance model (TAM) in the context of Jordan.Design/methodology/approachTo test the suggested research model, an online questionnaire was employed to collect data from 213 owners in Jordanian small and medium-sized enterprises (SMEs). The data were analyzed utilizing bootstrapped procedure by the partial least Squares-structural equation modeling (PLS-SEM).FindingsOut of ten postulated hypotheses, eight were accepted. Mainly, the empirical outcomes confirm the suggested hypotheses that the perceived usefulness (PUS) of AIS is positively and significantly impacted by perceived convenience (PCN) and perceived ease of use (PEU). Besides, the outcomes confirm that AIS usage is significantly influenced by PUS, PEU and perceived compatibility (PCM). Finally, the net benefits of AIS are positively influenced by AIS usage and information technology (IT) knowledge, whereby it was revealed that IT knowledge has a direct and indirect effect.Originality/valueThis study addresses a vital research gap in the literature by suggesting a comprehensive research model that can help garner enhanced usage of an AIS to obtain a better achievement among Jordanian SMEs performance.
Towards achieving SDGs through integrated reporting in Malaysian public universities
Authors: Mohammed Nor Farizal , Mahmud Radziah , Islam Md. Shafiqul , Mohamed Norhayati 
Published: November 2022

Purpose
The recent development in integrated reporting (<IR>) demonstrated a potential government tool for decision-making in allocating resources and developing sustainable policies for higher education institutions. This paper aims to examine the extent of the disclosure level of <IR> content elements in the annual reports of Malaysian Public Universities (MPUs). Additionally, the relationship between the disclosure level of <IR> content elements and the specific characteristics of MPUs is investigated.

Design/methodology/approach
The study performed descriptive statistics analysis for each component of <IR> content elements. This idea is achieved by examining the annual reports of MPUs between 2016 and 2018. The relationship was also investigated using ordinary least squares, fixed effect and lagged models.

Findings
The findings showed an increasing trend in the disclosure level of <IR> content elements in MPUs’ annual reports, supported by institutional theory. Furthermore, RUs exhibited a significant positive relationship with the disclosure level of <IR> content elements, whereas university size and report conciseness are insignificant variables.

Originality/value
The study adds to the body of knowledge in public sector accounting and has significant implications in the industry. This implication is specific to achieving sustainable development goals within the context of a developing country, paving avenues for further MPU reporting studies.
Making better IS security investment decisions: discovering the cost of data breach announcements during the COVID-19 pandemic
Authors: Dong Tianxi , Zhu Suning , Oliveira Mauro , Luo Xin (Robert) 
Published: November 2022

PurposeStock price reactions have often been used to evaluate the cost of data breaches in the current information systems (IS) security literature. To further this line of research, this study examines the impact of data breaches on stock returns, information asymmetry and unsystematic firm risk in the context of COVID-19.Design/methodology/approachThis paper employs an event study methodology and examines data breach events released in public databases, spanning pre- and post-COVID settings. This study investigated 283 data breaches of the US publicly traded firms, and the economic cost was measured by cumulative abnormal returns (CARs), trading volume, bid-ask spread and unsystematic risk.FindingsThe authors observe that data breaches during the COVID pandemic make investors react more negatively to data breach announcements, as reflected in the significantly negative difference in CARs between breached firms before COVID and those after COVID. The findings also indicate that, after the disclosure of data breach incidents, information asymmetry is reduced to a lesser extent compared with that in the pre-COVID setting. The authors also find that data breach events lead to an increase in the unsystematic risk of breached companies in the pre-COVID era but no change in the post-COVID era.Originality/valueThis study is the first effort to examine the economic consequences of data breaches by investigating the effects in the form of trading activities and risk measurement in the COVID setting.
Post-disaster tourism development and scenario planning for the quality of life of residents
Authors: Zhang Jun , Cheng Li 
Published: November 2022

Purpose
This study aims to explore the influence of postdisaster tourism development on the objective quality of life (QoL) of residents in Wenchuan County, simulate the long-run trend of postdisaster tourism development and QoL based on three proposed policy scenarios and formulate some practical suggestions to promote sustainable tourism development.

Design/methodology/approach
This study uses the system dynamic approach to develop a system dynamics (SD) model called “tourism–economy–environment–living” (TEEL) by using four subsystems: “the economic subsystem,” “the tourism subsystem,” “the environmental subsystem” and “the living subsystem.”

Findings
The results show that the influence of postdisaster tourism development on QoL is complex. Based on Butler’s destination life cycle theory, the influence of postdisaster tourism on objective QoL exhibits a nonlinear change closely tied to the development stages of tourist destinations. It showed that the QoL index increased after an initial decrease in the early stage (2009–2013) and then decreased in the later stage (2013–2019). Simulations of TEEL based on three different scenarios show that the current development path of tourism development is not ideal. The synergy scenario, highlighting the importance of the harmonious development of the TEEL, is the optimal scenario.

Originality/value
This study fills the gap in the literature on the influence of postdisaster tourism development on objective QoL from the perspective of SD. Modeling tourism development and objective QoL will contribute to a comprehensive understanding of whether and how tourism development can enhance residents’ QoL in disaster-affected areas.
Stable landings mask irreversible community reorganizations in an overexploited Mediterranean ecosystem
Authors: Sguotti Camilla , Bischoff Aurelia , Conversi Alessandra , Mazzoldi Carlotta , Möllmann Christian , Barausse Alberto 
Published: November 2022

Abstract

Cumulative human pressures and climate change can induce nonlinear discontinuous dynamics in ecosystems, known as regime shifts. Regime shifts typically imply hysteresis, a lacking or delayed system response when pressures are reverted, which can frustrate restoration efforts.

Here, we investigate whether the northern Adriatic Sea fish and macroinvertebrate community, as depicted by commercial fishery landings, has undergone regime shifts over the last 40 years, and the reversibility of such changes.

We use a stochastic cusp model to show that, under the interactive effect of fishing pressure and water warming, the community reorganized through discontinuous changes.

We found that part of the community has now reached a new stable state, implying that a recovery towards previous baselines might be impossible. Interestingly, total landings remained constant across decades, masking the low resilience of the community.

Our study reveals the importance of carefully assessing regime shifts and resilience in marine ecosystems under cumulative pressures and advocates for their inclusion into management.