bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Dopamine Assisted Synthesis of MoO3/Carbon Electrodes With Enhanced Capacitance in Aqueous Electrolyte
Authors: Norouzi Nazgol , Omo-Lamai Darrell , Alimohammadi Farbod , Averianov Timofey , Kuang Jason , Yan Shan , Wang Lei , Stavitski Eli , Leshchev Denis , Takeuchi Kenneth J. , Takeuchi Esther S. , Marschilok Amy C. , Bock David C. , Pomerantseva Ekaterina 
Published: April 2022

A capacitance increase phenomenon is observed for MoO3electrodes synthesizedviaa sol-gel process in the presence of dopamine hydrochloride (Dopa HCl) as compared to α-MoO3electrodes in 5M ZnCl2aqueous electrolyte. The synthesis approach is based on a hydrogen peroxide-initiated sol-gel reaction to which the Dopa HCl is added. The powder precursor (Dopa)xMoOy, is isolated from the metastable gel using freeze-drying. Hydrothermal treatment (HT) of the precursor results in the formation of MoO3accompanied by carbonization of the organic molecules; designated as HT-MoO3/C. HT of the precipitate formed in the absence of dopamine in the reaction produced α-MoO3, which was used as a reference material in this study (α-MoO3-ref). Scanning electron microscopy (SEM) images show a nanobelt morphology for both HT-MoO3/C and α-MoO3-ref powders, but with distinct differences in the shape of the nanobelts. The presence of carbonaceous content in the structure of HT-MoO3/C is confirmed by FTIR and Raman spectroscopy measurements. X-ray diffraction (XRD) and Rietveld refinement analysis demonstrate the presence of α-MoO3and h-MoO3phases in the structure of HT-MoO3/C. The increased specific capacitance delivered by the HT-MoO3/C electrode as compared to the α-MoO3-ref electrode in 5M ZnCl2electrolyte in a −0.25–0.70 V vs. Ag/AgCl potential window triggered a more detailed study in an expanded potential window. In the 5M ZnCl2electrolyte at a scan rate of 2 mV s−1, the HT-MoO3/C electrode shows a second cycle capacitance of 347.6 F g−1. The higher electrochemical performance of the HT-MoO3/C electrode can be attributed to the presence of carbon in its structure, which can facilitate electron transport. Our study provides a new route for further development of metal oxides for energy storage applications.
Effect on Soil Properties and Crop Yields to Long-Term Application of Superabsorbent Polymer and Manure
Authors: Yang Yonghui , Wu Jicheng , Du Yan-Lei , Gao Cuimin , Tang Darrell W. S. , van der Ploeg Martine 
Published: April 2022

Intensive farming leads to soil degradation, and poses a serious threat to agricultural yield sustainability and soil ecology. Superabsorbent polymer (SAP) and organic manure (OM) are amendments that can remediate the soil, and improve soil structure and soil pore characteristics, thereby enhancing soil quality and promoting crop growth. These benefits may be enhanced through long-term continuous application of the amendments. X-ray computed tomography (CT) was used to determine the number, connectivity, and locations of pores (160–1,000 μm, &gt;1,000 µm) in the soil at depths of 0–100 cm. Soil organic carbon content, proportion of water-stable macroaggregates (&gt;0.25 mm) (WSM), soil bulk density, field water capacity, available water content, wilting point content, and saturated hydraulic conductivity were also determined. Compared to the control, long-term SAP and OM treatments significantly increased the number of 160–1,000 µm and &gt;1,000 µm soil pores (5–20 cm depths), &gt;1,000 µm and &gt;160 µm soil porosity (at 0–20 cm depths), total organic carbon (10–20 cm and 40–50 cm), field water capacity (0–20 cm), available water content (0–20 cm), wilting point (10–30 cm), saturated water content (10–70 cm), saturated hydraulic conductivity (0–90 cm), and WSM (0–30 cm and 40–50 cm) while decreasing the soil bulk density (0–70 cm). Compared to OM, SAP had greater beneficial effects on the total organic carbon, soil field water capacity, available water content, saturated water content, saturated hydraulic conductivity, and WSM in soil at 0–20 cm depth. The improvements to soil quality resulted in an increase in the yields of wheat and maize. Therefore, although SAP and OM were both effective at improving soil properties and increasing the economic yield of wheat and maize, the effectiveness of SAP was greater than that of OM.
Prediction of the Central Indian Ocean Mode in S2S Models
Authors: Qin Jianhuang , Zhou Lei , Li Baosheng , Meng Ze 
Published: April 2022

Prediction of precipitation during the Indian summer monsoon (ISM) is a persistent scientific challenge. The central Indian Ocean (CIO) mode was proposed as a subseasonal climate mode over the tropical Indian Ocean, and it has a close relation with monsoon intraseasonal oscillations (MISO) during the ISM both in observations and simulations. In this study, the prediction skill of the CIO mode in the subseasonal-to-seasonal (S2S) air–sea coupled models is examined. The ECMWF and UKMO models display significantly higher skills for up to about 2 and 3 weeks, respectively, which are longer than other S2S models. The decline of the CIO mode prediction skill is due to the reduced signal of subseasonal zonal winds at 850 hPa over the tropical central Indian Ocean (especially along the equator; 5°S–5°N, 70°E–85°E). Therefore, a better simulation of tropical subseasonal zonal winds is required to improve the CIO mode prediction in models, and the improvement will benefit a better MISO simulation and a higher prediction skill during the ISM.
Kelp (Saccharina latissima) Mitigates Coastal Ocean Acidification and Increases the Growth of North Atlantic Bivalves in Lab Experiments and on an Oyster Farm
Authors: Young Craig S. , Sylvers Laine H. , Tomasetti Stephen J. , Lundstrom Andrew , Schenone Craig , Doall Michael H. , Gobler Christopher J. 
Published: April 2022

Coastal zones can be focal points of acidification where the influx of atmospheric CO2 can be compounded by additional sources of acidity that may collectively impair calcifying organisms. While the photosynthetic action of macrophytes may buffer against coastal ocean acidification, such activity has not been well-studied, particularly among aquacultured seaweeds. Here, we report on field and laboratory experiments performed with North Atlantic populations of juvenile hard clams (Mercenaria mercenaria), eastern oysters (Crassostrea virginica), and blue mussels (Mytilus edulis) grown with and without increased CO2 and with and without North Atlantic kelp (Saccharina latissima) over a range of aquaculture densities (0.3 – 2 g L-1). In all laboratory experiments, exposure to elevated pCO2 (&gt;1,800 µatm) resulted in significantly reduced shell- and/or tissue-based growth rates of bivalves relative to control conditions. This impairment was fully mitigated when bivalves were exposed to the same acidification source but also co-cultured with kelp. Saturation states of aragonite were transformed from undersaturated to saturated in the acidification treatments with kelp present, while the acidification treatments remained undersaturated. In a field experiment, oysters grown near aquacultured kelp were exposed to higher pH waters and experienced significantly faster shell and tissue based growth rates compared to individuals grown at sites away from kelp. Collectively, these results suggest that photosynthesis by S. latissima grown at densities associated with aquaculture increased pH and decreased pCO2, fostering a carbonate chemistry regime that maximized the growth of juvenile bivalves. As S. latissima has been shown to benefit from increased CO2, growing bivalves and kelp together under current or future acidification scenarios may be a synergistically beneficial integrated, multi-trophic aquaculture approach.
SPP1 and CXCL9 Promote Non-alcoholic Steatohepatitis Progression Based on Bioinformatics Analysis and Experimental Studies
Authors: Wang Wen , Liu Xiaojing , Wei Peiyao , Ye Feng , Chen Yunru , Shi Lei , Zhang Xi , Li Jianzhou , Lin Shumei , Yang Xueliang 
Published: April 2022

Background and AimsNon-alcoholic fatty liver disease (NAFLD) is a major chronic liver disease worldwide, and non-alcoholic steatohepatitis (NASH) is one of its pathological subtypes. The pathogenesis of NASH has not yet been fully elucidated. The purpose of this study was to identify the hub genes and pathways involved in NASH using bioinformatics methods. The hub genes were confirmed in human and animal models.Materials and MethodsThree Gene Expression Omnibus (GEO) datasets (GSE48452, GSE58979, and GSE151158) of NASH patients and healthy controls were included in the study. We used GEO2R to identify differentially expressed genes (DEGs) between NASH patients and healthy controls. Functional enrichment analyses were then performed to explore the potential functions and pathways of the DEGs. In all DEGs, only two genes were highly expressed in NASH patients throughout the three datasets; these two genes, SPP1 and CXCL9, were further studied. Serum and liver tissues from NASH patients and healthy controls were collected. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured in NASH patients and healthy controls. Liver tissues were stained with hematoxylin and eosin. Immunohistochemical staining was used to evaluate the expression levels of the two genes in liver tissues. Male C57BL/6J mice were fed a methionine choline-deficient (MCD) diet for 8 weeks, after which serum ALT and AST levels were measured and liver tissues were stained.ResultsSPP1 and CXCL9 were the hub genes detected in the three datasets. “Lipid metabolism,” “inflammatory response,” and “lymphocyte activation” were the most significant biological functions in GSE48452, GSE58979, and GSE151158, respectively. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the toll-like receptor signaling pathway was significantly enriched in NASH patients. Serum ALT and AST levels were significantly increased in NASH patients compared to healthy controls. Liver tissues had more serious steatosis, hepatocyte ballooning degeneration, and lobular inflammatory infiltration, and the expression of SPP1 and CXCL9 in liver cells was significantly upregulated in NASH patients compared to healthy controls. MCD diet mice were consistent with NASH patients.ConclusionSPP1 and CXCL9 may play important roles in NASH pathogenesis and could be potential therapeutic targets and biomarkers of NASH in the future. Further experimental studies are needed to confirm our results.
Identification of Tumor Microenvironment and DNA Methylation-Related Prognostic Signature for Predicting Clinical Outcomes and Therapeutic Responses in Cervical Cancer
Authors: Liu Bangquan , Zhai Jiabao , Wang Wanyu , Liu Tianyu , Liu Chang , Zhu Xiaojie , Wang Qi , Tian Wenjing , Zhang Fubin 
Published: April 2022

Background: Tumor microenvironment (TME) has been reported to have a strong association with tumor progression and therapeutic outcome, and epigenetic modifications such as DNA methylation can affect TMB and play an indispensable role in tumorigenesis. However, the potential mechanisms of TME and DNA methylation remain unclear in cervical cancer (CC).Methods: The immune and stromal scores of TME were generated by the ESTIMATE algorithm for CC patients in The Cancer Genome Atlas (TCGA) database. The TME and DNA methylation-related genes were identified by the integrative analysis of DNA promoter methylation and gene expression. The least absolute shrinkage and selection operator (LASSO) Cox regression was performed 1,000 times to further identify a nine-gene TME and DNA methylation-related prognostic signature. The signature was further validated in Gene Expression Omnibus (GEO) dataset. Then, the identified signature was integrated with the Federation International of Gynecology and Obstetrics (FIGO) stage to establish a composite prognostic nomogram.Results: CC patients with high immunity levels have better survival than those with low immunity levels. Both in the training and validation datasets, the risk score of the signature was an independent prognosis factor. The composite nomogram showed higher accuracy of prognosis and greater net benefits than the FIGO stage and the signature. The high-risk group had a significantly higher fraction of genome altered than the low-risk group. Eleven genes were significantly different in mutation frequencies between the high- and low-risk groups. Interestingly, patients with mutant TTN had better overall survival (OS) than those with wild type. Patients in the low-risk group had significantly higher tumor mutational burden (TMB) than those in the high-risk group. Taken together, the results of TMB, immunophenoscore (IPS), and tumor immune dysfunction and exclusion (TIDE) score suggested that patients in the low-risk group may have greater immunotherapy benefits. Finally, four drugs (panobinostat, lenvatinib, everolimus, and temsirolimus) were found to have potential therapeutic implications for patients with a high-risk score.Conclusions: Our findings highlight that the TME and DNA methylation-related prognostic signature can accurately predict the prognosis of CC and may be important for stratified management of patients and precision targeted therapy.
Brain Gray Matter Alterations in Hepatic Encephalopathy: A Voxel-Based Meta-Analysis of Whole-Brain Studies
Authors: Zhu Licheng , Zhang Weihua , Chen Lei , Ren Yanqiao , Cao Yanyan , Sun Tao , Sun Bo , Liu Jia , Wang Jing , Zheng Chuansheng 
Published: April 2022

BackgroundPrevious studies on voxel-based morphometry (VBM) have found that there were gray matter alterations in patients with hepatic encephalopathy (HE). However, the reported results were inconsistent and lack a quantitative review. Therefore, this study aims for a quantitative meta-analysis of VBM analysis on patients with HE.MethodsThe studies in our meta-analysis were collected from Pubmed, Web of Science, and Embase, which were published from January 1947 to October 2021. The seed-based d mapping (SDM) method was applied to quantitatively estimate the regional gray matter abnormalities in patients with HE. A meta-regression analysis was applied to evaluate the relationship between plasma ammonia and gray matter alteration.ResultsThere were nine studies, with sixteen datasets consisting of 333 participants with HE and 429 healthy controls. The pooled and subgroup meta-analyses showed an increase in gray matter volume (GMV) in the bilateral thalamus and the calcarine fissure but a decrease in the GMV in the bilateral insula, the basal ganglia, the anterior cingulate gyrus, and the cerebellum. The meta-regression showed that plasma ammonia was positively associated with the GMV in the left thalamus but was negatively associated with the GMV in the cerebellum and the bilateral striatum.ConclusionGray matter volume in patients with HE largely varied and could be affected by plasma ammonia. The findings of this study could help us to better understand the pathophysiology of cognitive dysfunction in patients with HE.
Sleep During Oncological Treatment – A Systematic Review and Meta-Analysis of Associations With Treatment Response, Time to Progression and Survival
Authors: Strøm Louise , Danielsen Josefine T. , Amidi Ali , Cardenas Egusquiza Ana Lucia , Wu Lisa Maria , Zachariae Robert 
Published: April 2022

IntroductionDisrupted sleep and sleep-wake activity are frequently observed in cancer patients undergoing oncological treatment. These disruptions are often associated with aggravated symptom burden and diminished health-related quality of life that in turn may compromise treatment adherence and, thus, effectiveness. In addition, disrupted sleep has been linked to carcinogenic processes, which ultimately could result in worse prognostic outcomes.AimsOur aim was to systematically review and conduct a meta-analysis of studies examining the associations between sleep and sleep-wake activity and prognostic outcomes in cancer patients undergoing oncological treatment.MethodsA comprehensive systematic search of English language papers was undertaken in June 2020 using PubMed, The Cochrane Library, and CINAHL. Two reviewers independently screened 4,879 abstracts. A total of 26 papers were included in the narrative review. Thirteen papers reporting hazard ratios reflecting associations between a dichotomized predictor variable (sleep) and prognostic outcomes were subjected to meta-analysis.ResultsNineteen of the 26 eligible studies on a total of 7,092 cancer patients reported associations between poorer sleep and poorer response to treatment, shorter time to progression, and/or reduced overall survival, but were highly heterogeneous with respect to the sleep and outcome parameters investigated. Meta-analysis revealed statistically significant associations between poor self-reported sleep and reduced overall survival (HR = 1.33 [95% CI 1.09–1.62], k = 11), and shorter time to progression (HR = 1.40 [95% CI 1.23–1.59], k = 3) and between poor objectively assessed sleep and reduced overall survival (HR = 1.74 [95% CI 1.05–2.88], k = 4).ConclusionThe current findings indicate that disturbed sleep during treatment may be a relevant behavioral marker of poor cancer prognosis. The limited number of studies, the common use of single item sleep measures, and potential publication bias highlight the need for further high quality and longitudinal studies.
Knowledge, Attitudes, and Practices of NICU Doctors and Nurses Toward Prevention and Control of Nosocomial Infection With Multidrug Resistant Organism
Authors: Zhou Jinyan , Chen Shuohui 
Published: April 2022

BackgroundNosocomial infection with multidrug resistant organisms (MDRO) can directly influence the curative effect and the prognosis of neonates, bringing great difficulties to clinical treatment. As direct contacts of neonates, the knowledge, attitudes, and practices (KAP) of doctors and nurses are critical for the prevention and control of MDRO infection in neonatal intensive care units (NICUs).PurposeThis study describes the KAP of doctors and nurses in NICUs toward the prevention and control of nosocomial infection with MDRO and analyzes its influencing factors.MethodsThis cross-sectional study used convenience sampling to survey 397 doctors and nurses from the NICU of 28 hospitals in 11 cities in Zhejiang Province, China. A univariate analysis and a multiple linear regression were used to analyze the data.ResultsThe median scoring rate (interquartile range) of the knowledge, attitude, and practice questionnaire was 0.86 (0.82–0.91), 0.98 (0.91–1.00), and 0.995 (0.97–1.00), respectively. However, the median scoring rate regarding “basic knowledge of MDRO” and “special prevention and control measures” was 0.50 in knowledge. The multiple linear regression showed that the influencing factors of KAP were hospital grade, professional title, gender, regular supervision, and training.ConclusionsThere was still room for improvement in the KAP of NICU doctors and nurses, especially regarding the knowledge. Men, doctors and nurses in Grade II hospitals, and doctors and nurses with primary professional titles had worse KAP. Training and supervision helped improve the KAP.Relevance to Clinical PracticeTo improve the KAP of doctors and nurses to enhance the prevention and control effect for the MDRO infection in NICUs, hospitals and departments should carry out targeted training and strengthen supervision, while Grade II hospitals, men, and doctors and nurses with primary professional titles need more attention.
Indoor Pool Game and Substance Abuse as Trajectories to Students’ Academic Procrastination: The Mediation Role of Self-Regulation
Authors: Gita Dinaol Urgessa , Koya Amanuel Tadesse , Worku Berhanu Nigussie 
Published: April 2022

BackgroundOver the last decade, indoor pool games (IPGs) and substance abuse (SA) became a remarkable emerging addictive behavior among adolescent university students. With the failure of educational quality and retention of learners, boomerangs around the university local environment in line with the students’ learning culture were not considered in many countries including Ethiopia. Thus, this study aimed to examine the trajectory and contribution of an IPG and SA to students’ academic procrastination (AP) as determinants of quality education.MethodsA sequential explanatory mixed-methods design was employed. Self-reporting questionnaires, interview guides, and an observation checklist were used to collect data. All self-reporting items were adapted from previous scales. By using simple random sampling techniques, 237 undergraduate university students were selected for obtaining the quantitative data, and using purposive sampling, 12 interviewees were selected to collect the qualitative data. The SPSS AMOS version 25 was used to compute the multiple mediation path analysis. The Hayes PROCESS macro model was used. Furthermore, the thematic content analysis method was employed for the qualitative data.ResultsA direct path analysis was established between IPG, SA, and AP. The path analysis model indicated that IPG did not significantly predict AP. Moreover, SA significantly predicted AP. In addition, SR had a partial mediating effect on the relationship between IPG, SA, and AP.ConclusionThe study concluded that IPG and substances available around the university local environment found trajectories to students’ AP, which in turn affects the quality of education.
Discovery of New Secondary Metabolites from Marine Bacteria Hahella Based on an Omics Strategy
Authors: He Shufen , Li Peishan , Wang Jingxuan , Zhang Yanzhu , Lu Hongmei , Shi Liufei , Huang Tao , Zhang Weiyan , Ding Lijian , He Shan , Liu Liwei 
Published: April 2022

Hahella is one characteristic genus under the Hahellaceae family and shows a good potential for synthesizing new natural products. In this study, we examined the distribution of the secondary metabolite biosynthetic gene cluster (SMBGC) under Hahella with anti-SMASH. The results derived from five genomes released 70 SMBGCs. On average, each strain contains 12 gene clusters, and the most abundant ones (45.7%) are from the family of non-ribosomal peptide synthetase (NRPS) and non-ribosomal peptide synthetase hybrid with polyketide synthase (NRPS/PKS), indicating a great potential to find bioactive compounds. The comparison of SMBGC between H. chejuensis and other species showed that H. chejuensis contained two times more gene clusters than H. ganghwensis. One strain, designed as NBU794, was isolated from the mangrove soil of Dongzhai Port in Haikou (China) by iChip. The 16S rRNA gene of NBU794 exhibited 99% identity to H. chejuensis KCTC 2396 and clustered with the H. chejuensis clade on the phylogenetic trees. Genome mining on strain NBU794 released 17 SMBGCs and two groups of bioactive compounds, which are chejuenolide A-C and nine prodiginines derivatives. The prodiginines derivatives include the well-known lead compound prodigiosin and two new compounds, 2-methyl-3-pentyl-4-O-methyl-prodiginine and 2-methyl-3-octyl-prodiginine, which were identified through fragmentation analysis based on LC-MS/MS. The anti-microbial activity assay showed prodigiosin and 2-methyl-3-heptyl-prodiginine exhibited the best performance in inhibiting Escherichia coli, Salmonella paratyphi B, MASA Staphylococcus aureus, Bacillus subtilis, and Candida albicans. Moreover, the yield of prodigiosin in H. chejuensis NBU794 was also evaluated, which could reach 1.40 g/L under the non-optimized condition and increase to 5.83 g/L in the modified ISP4 medium with macroporous adsorption beads added, indicating that NBU794 is a promising source of prodigiosin.