bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased expression of angiopoietin‐like protein 4 regulates matrix metalloproteinase‐13 expression in Porphyromonas gingivalis lipopolysaccharides‐stimulated gingival fibroblasts and ligature‐induced experimental periodontitis
Authors: Kondo Shun , Kojima Kento , Nakamura Nobuhisa , Miyabe Megumi , Kikuchi Takeshi , Ohno Tasuku , Sawada Noritaka , Minato Tomomi , Saiki Tomokazu , Ito Mizuho , Sasajima Sachiko , Matsubara Tatsuaki , Mitani Akio , Naruse Keiko 
Published: November 2022

AbstractBackgroundAngiopoietin‐like protein 4 (ANGPTL4) is produced in chronic or acute inflammation. Although ANGPTL4 increases in the periodontal ligament fibroblasts during hypoxia, the involvement and role of ANGPTL4 in periodontitis have not been elucidated.ObjectiveIn this study, we investigated whether ligature‐induced experimental periodontitis and/or Porphyromonas gingivalis lipopolysaccharides (Pg‐LPS) would upregulate ANGPTL4 expression and whether ANGPTL4 would somehow involve in the expression of matrix metalloproteinases (MMPs) which are key molecules in the process of periodontal tissue destruction.MethodsExperimental periodontitis was induced in 6‐week‐old male Sprague–Dawley rats by placing a nylon suture around the neck of the maxillary second molar. Two weeks after the induction of periodontitis, the periodontal tissue was excised and analyzed by histological/immunohistochemical staining and gene expression analyses. Human gingival fibroblasts (hGFs) were stimulated with Pg‐LPS. The gene expression of ANGPTLs and receptors involved in ANGPTL4 recognition were observed. We also confirmed the changes in gene expression of MMPs upon stimulation with human ANGPTL4. Furthermore, we downregulated ANGPTL4 expression by short interfering RNA in hGFs and investigated the effect of Pg‐LPS on MMP production.ResultsInduction of periodontitis significantly increased the expression of ANGPTL4 in the gingiva. Pg‐LPS significantly increased the gene and protein expression of ANGPTL4 in hGFs but not the gene expression of other ANGPTLs or ANGPTL receptors. Recombinant human ANGPTL4 significantly increased MMP13 gene expression in hGFs. We also confirmed that MMP13 expression was increased in the gingiva during experimental periodontitis. Pg‐LPS induced MMP13 gene expression in hGFs. These results suggest the pivotal role of ANGPTL4 in periodontitis.ConclusionPeriodontitis increases ANGPTL4 expression in the gingiva, further suggesting that increased ANGPTL4 may be a factor involved in enhancing MMP13 expression.
Thermochemical evolution of the acapulcoite–lodranite parent body: Evidence for fragmentation‐disrupted partial differentiation
Authors: Lucas Michael P. , Dygert Nicholas , Ren Jialong , Hesse Marc A. , Miller Nathaniel R. , McSween Harry Y. 
Published: November 2022

AbstractPrimitive achondrites of the acapulcoite–lodranite clan (ALC) are residues of partial melting that displays a continuum of thermal metamorphism and partial melting most likely set by burial depth within an internally heated, primordial acapulcoite–lodranite parent body (ALPB). New major and trace element data from eight ALC meteorites and the application of several thermometric methods suggest that the ALPB was affected by partial differentiation disrupted by rapid cooling from peak, magmatic temperatures. Application of rare earth element‐in‐two‐pyroxene thermometry recovers temperatures of 1125–1250 °C for lodranites, while two‐pyroxene solvus and Ca‐in‐olivine thermometry recover lower temperatures for ALC meteorites (941–1114 °C and 686–850 °C, respectively). Major and trace element disequilibrium in acapulcoite and transitional groups provides evidence for cryptic melt infiltration and melt rock reaction within these layers of the ALPB. From lodranites, we determined rapid cooling rates of ~1 to ~26 °C yr−1from peak temperatures, consistent with collisional fragmentation of the parent body during differentiation. After this initial period of rapid cooling, cooling rates decreased by two to four orders of magnitude through Ca‐in‐olivine closure temperatures (~750 °C). We hypothesize that the primordial ALPB possessed an onion shell‐type layered structure that was disrupted by collisional breakup during partial differentiation. Thermal modeling suggests that ALC samples originate from ~300 m to ~10 km radius collisional fragments that cooled rapidly over time scales of several to ~20,000 yr, then reaccreted to form a slower cooling, second‐generation rubble‐pile asteroid. The source of ALC meteorites is a second‐generation (or later) rubble‐pile body of S‐type spectral class located near the Jupiter 3:1 mean motion resonance in the Main Belt of asteroids.
A hierarchical framework for improving ride comfort of autonomous vehicles via deep reinforcement learning with external knowledge
Authors: Du Yuchuan , Chen Jing , Zhao Cong , Liao Feixiong , Zhu Meixin 
Published: November 2022

AbstractRide comfort plays an important role in determining the public acceptance of autonomous vehicles (AVs). Many factors, such as road profile, driving speed, and suspension system, influence the ride comfort of AVs. This study proposes a hierarchical framework for improving ride comfort by integrating speed planning and suspension control in a vehicle‐to‐everything environment. Based on safe, comfortable, and efficient speed planning via dynamic programming, a deep reinforcement learning‐based suspension control is proposed to adapt to the changing pavement conditions. Specifically, a deep deterministic policy gradient with external knowledge (EK‐DDPG) algorithm is designed for the efficient self‐adaptation of suspension control strategies. The external knowledge of action selection and value estimation from other AVs are combined into the loss functions of the DDPG algorithm. In numerical experiments, real‐world pavements detected in 11 districts of Shanghai, China, are applied to verify the proposed method. Experimental results demonstrate that the EK‐DDPG‐based suspension control improves ride comfort on untrained rough pavements by 27.95% and 3.32%, compared to a model predictive control (MPC) baseline and a DDPG baseline, respectively. Meanwhile, the EK‐DDPG‐based suspension control improves computational efficiency by 22.97%, compared to the MPC baseline, and performs at the same level as the DDPD baseline. This study provides a generalized and computationally efficient approach for improving the ride comfort of AVs.
Phytochrome B regulates reactive oxygen signaling during abiotic and biotic stress in plants
Authors: Fichman Yosef , Xiong Haiyan , Sengupta Soham , Morrow Johanna , Loog Hailey , Azad Rajeev K. , Hibberd Julian M. , Liscum Emmanuel , Mittler Ron 
Published: November 2022

Summary
Reactive oxygen species (ROS) and the photoreceptor protein phytochrome B (phyB) play a key role in plant acclimation to stress. However, how phyB that primarily functions in the nuclei impacts ROS signaling mediated by respiratory burst oxidase homolog (RBOH) proteins that reside on the plasma membrane, during stress, is unknown.
Arabidopsis thaliana and Oryza sativa mutants, RNA‐Seq, bioinformatics, biochemistry, molecular biology, and whole‐plant ROS imaging were used to address this question.
Here, we reveal that phyB and RBOHs function as part of a key regulatory module that controls apoplastic ROS production, stress‐response transcript expression, and plant acclimation in response to excess light stress. We further show that phyB can regulate ROS production during stress even if it is restricted to the cytosol and that phyB, respiratory burst oxidase protein D (RBOHD), and respiratory burst oxidase protein F (RBOHF) coregulate thousands of transcripts in response to light stress. Surprisingly, we found that phyB is also required for ROS accumulation in response to heat, wounding, cold, and bacterial infection.
Our findings reveal that phyB plays a canonical role in plant responses to biotic and abiotic stresses, regulating apoplastic ROS production, possibly while at the cytosol, and that phyB and RBOHD/RBOHF function in the same regulatory pathway.
Whose techno‐utopia? Power, privilege, and the religio‐secular frameworks of the contemporary biohacking movement
Authors: Rifai Emma 
Published: November 2022

AbstractCommitted to providing a form of this‐worldly salvation to its largely white, male, and wealthy practitioners, biohacking is a popular “citizen science” movement that applies a life‐hacking mentality to the intimate spaces of the body by embracing DIY biology. Exploring the contours of its power and privilege in the United States today, this essay works to historicize the contemporary biohacking movement by attending to discourses produced by some of its key personalities, its depictions in popular media, and to the growing body of academic scholarship that seeks to theorize biohacking's logics and rhetorics. After first constructing a basic genealogy and typology of the movement, this essay then considers the religio‐secular frameworks that shape biohacking's unique form of techno‐utopia. In particular, it examines what kind of future individuals are being promised in a world where they are invited to become superhuman, if they have the time and resources to pursue immortality.
Flux Weakening Operation Based Design of an Integrated Electrohydraulic Axial Piston Unit
Authors: Sarode Shanmukh , Shang Lizhi , Vacca Andrea , Sudhoff Scott D. 
Published: November 2022

Abstract
With the recent electrification trends affecting mobile hydraulics, there is a rising demand for the development of energy-efficient and compact hydraulic supply units driven by electric machines. Such units capable of multi-quadrant operation are commonly known as electrohydraulic units (EHUs). Owing to inherent differences in the power densities of the two machines, efforts are required to make more compact electric machines in order to reduce the overall size of the resulting EHU. This paper discusses the optimal design of such an integrated EHU with a radial flux permanent magnet synchronous machine with flux weakening operation for a swashplate type axial piston machine. A flux weakening mode current control strategy extends the operating speeds of the electric machine to its maximum power by injecting a negative d-axis current.
Such a flux weakening mode of operation can allow optimal sizing of the EHUs if the peak flow and pressure demands do not coincide. Based on a given work cycle and a reference hydraulic unit, a multi-objective genetic algorithm based design optimization is used to optimize the electric machine of the integrated EHU for the best efficiency and compactness. The EM design with flux weakening mode of operation are compared to the ones with max torque per amp mode of operation in terms of mass, torque density, and efficiency. Flux weakening based electric machine design allows sizing for maximum achievable power and helps not only to downsize the electric machine by 30% but also to save on the cost of the power electronics required.
National levels, changes and correlates of ideal cardiovascular health among Beninese adults: evidence from the 2008 to 2015 STEPS surveys
Authors: Kaboré Michael , Hien Yéri Esther , Fassinou Lucresse Corine , Cissé Kadari , Ngwasiri Calypse , Coppieters Yves , Kirakoya-Samadoulougou Fati 
Published: November 2022

IntroductionA higher number of ideal cardiovascular health (CVH) metrics is associated with a lower risk of cardiovascular-related and all-cause mortality. However, the change in CVH metrics has rarely been studied in sub-Saharan Africa. We investigated the level and changes of CVH metrics and their correlates among Beninese adults between 2008 and 2015.MethodsSecondary analysis was performed on data obtained from Benin’s 2008 and 2015 WHO Stepwise surveys (STEPS). In total, 3617 and 3768 participants aged 25–64 years were included from both surveys, respectively. CVH metrics were assessed using the American Heart Association definition, which categorised smoking, fruit and vegetable consumption, physical activity, body mass index (BMI), blood pressure (BP), total cholesterol (TC) and glycaemia into ‘ideal’, ‘intermediate’ and ‘poor’ CVH. The prevalence of ideal CVH metrics was standardised using the age and sex structure of the 2013 population census.ResultsFew participants met all seven ideal CVH metrics, and ideal CVH significantly declined between 2008 and 2015 (7.1% (95% CI 6.1% to 8.1%) and 1.2% (95% CI 0.8% to 1.5%), respectively). The level of poor smoking (8.0% (95% CI 7.1% to 8.9%) and 5.6% (95% CI 4.8% to 6.3%)) had decreased, whereas that of poor BP (25.9% (95% CI 24.5% to 27.4%) and 32.0% (95% CI 30.0% to 33.5%)), poor total cholesterol (1.5% (95% CI 1.0% to 1.9%) and 5.5% (95% CI 4.8% to 6.2%)) and poor fruit and vegetable consumption (34.2% (95% CI 32.4% to 35.9%) and 51.4% (95% CI 49.8% to 53.0%)) significantly increased. Rural residents and young adults (25–34 years) had better CVH metrics.ConclusionThe proportion of adults with ideal CVH status was low and declined significantly between 2008 and 2015 in Benin, emphasising the need for primordial prevention targeting urban areas and older people to reduce the burden of cardiovascular disease risk factors.
ImmunoPET: IMaging of cancer imMUNOtherapy targets with positron Emission Tomography: a phase 0/1 study characterising PD-L1 with89Zr-durvalumab (MEDI4736) PET/CT in stage III NSCLC patients receiving chemoradiation study protocol
Authors: Hegi-Johnson Fiona , Rudd Stacey E , Wichmann Christian , Akhurst Tim , Roselt Peter , Trinh Jenny , John Thomas , Devereux Lisa , Donnelly Paul S , Hicks Rod , Scott Andrew M , Steinfort Daniel , Fox Stephen , Blyth Benjamin , Parakh Sagun , Hanna Gerard G , Callahan Jason , Burbury Kate , MacManus Michael 
Published: November 2022

BackgroundImmunoPET is a multicentre, single arm, phase 0–1 study that aims to establish if89Zr-durvalumab PET/CT can be used to interrogate the expression of PD-L1 in larger, multicentre clinical trials.MethodsThe phase 0 study recruited 5 PD-L1+ patients with metastatic non-small cell lung cancer (NSCLC). Patients received 60MBq/70 kg89Zr-durva up to a maximum of 74 MBq, with scan acquisition at days 0, 1, 3 or 5±1 day. Data on (1) Percentage of injected89Zr-durva dose found in organs of interest (2) Absorbed organ doses (µSv/MBq of administered89Zr-durva) and (3) whole-body dose expressed as mSv/100MBq of administered dose was collected to characterise biodistribution.The phase 1 study will recruit 20 patients undergoing concurrent chemoradiotherapy for stage III NSCLC. Patients will have89Zr-durva and FDG-PET/CT before, during and after chemoradiation. In order to establish the feasibility of89Zr-durva PET/CT for larger multicentre trials, we will collect both imaging and toxicity data. Feasibility will be deemed to have been met if more than 80% of patients are able complete all trial requirements with no significant toxicity.Ethics and disseminationThis phase 0 study has ethics approval (HREC/65450/PMCC 20/100) and is registered on the Australian Clinical Trials Network (ACTRN12621000171819). The protocol, technical and clinical data will be disseminated by conference presentations and publications. Any modifications to the protocol will be formally documented by administrative letters and must be submitted to the approving HREC for review and approval.Trial registration numberAustralian Clinical Trials Network ACTRN12621000171819.
Friendship during patients’ stable and unstable phases of incurable cancer: a qualitative interview study
Authors: van Eijk Michelle , de Vries Daniel H , Sonke Gabe S , Buiting Hilde M 
Published: November 2022

ObjectivesLittle is known about the added value of friendship during the care of intensive cancer disease trajectories. Friends, however, can play an important (caring-)role to increase their friends’ (mental) well-being. We explored the experiences and desires of friends while their ill friends were—most of the time—in a stable phase of incurable cancer.DesignQualitative study in the Netherlands based on 14 in-depth interviews with friends of patients living with incurable cancer. Interviews were performed at the home setting or the friend’s office. Data gathering was inspired by grounded theory and analysed with a thematic analysis.SettingThe home setting/friend’s office.ResultsFriends reported to experience difficulties in how and how often they wanted to approach their ill friends. They emphasised the ever-present knowledge of cancer inside their friends’ body as a ‘time bomb’. They seemed to balance between the wish to take care for their ill friend, having a good time and not knowing what their ill friend desired at specific times. Some friends felt burdened with or forced to provide more care than they could, although they acknowledged that this relationship provided space to reflect about their own life.ConclusionsFriends are constantly negotiating and renegotiating their relationship depending on the severity of the disease, transparency of patients about their illness, their previous experiences and personal circumstances in life. Although a decrease in friendship may impact a patient’s quality of life, friends also need to be protected against providing more care than they are willing or able to give. Healthcare professionals, being aware of this phenomenon, can assist in this.
Point-of-care biomarkers for prediction of kidney function trajectory among sugarcane cutters: a comparative test accuracy study
Authors: Hansson Erik , Wesseling Catharina , Wegman David , Ekström Ulf , Chavarria Denis , Glaser Jason , Jakobsson Kristina 
Published: November 2022

ObjectivesHeat-stressed Mesoamerican workers, such as sugarcane cutters, suffer from high rates of chronic kidney disease of non-traditional origin (CKDnt). We aimed to identify easily available early markers of rapid kidney function decline in a population at high risk of CKDnt.DesignThe accuracy of different biomarkers measured during harvest for prediction of cross-harvest kidney function decline were assessed in an exploratory study group, and the performance of the most promising biomarker was then assessed in an independent confirmation group.SettingMale sugarcane cutters in El Salvador and Nicaragua.Participants39 male Salvadoran sugarcane cutters sampled fortnightly at ≤9 occasions before and after work shift during harvest. 371 male Nicaraguan sugarcane cutters were sampled as part of routine monitoring during two harvests. Cutters worked at high physical intensity at wet-bulb globe temperatures mostly above 29°C for 6–8 hours per day 6 days a week during the 5–6 months harvest season.Primary outcomesChange in estimated glomerular filtration rate (CKD Epidemiology Collaboration) across the harvest season (ΔeGFRcross-harvest).ResultsDipstick leukocyturia after work shift in the El Salvadoran group was the most promising marker, explaining >25% of ΔeGFRcross-harvestvariance at 8/9 occasions during harvest. Leukocyturia was associated with experiencing fever, little or dark urine, cramps, headache, dizziness and abdominal pain in the preceding 2-week period. Decreasing blood haemoglobin (Hb) and eGFR during harvest were also predictive of ΔeGFRcross-harvest. In the Nicaraguan confirmation dataset, those having ≥++ leukocyturia at any sampling during harvest had a 13 mL/min/1.73 m2(95% CI 10 to 16 mL/min/1.73 m2) worse ΔeGFRcross-harvestthan those without recorded leukocyturia.ConclusionLeukocyturia and Hb, both measurable with point-of-care methods, may be early indicators for kidney injury and risk for eGFR decline among heat-stressed male workers, thereby facilitating individual-level prevention and research aiming to understand the causes of CKDnt.
Predicting mortality and morbidity in emergency general surgery patients in a Jordanian Tertiary Medical Center: a retrospective validation study of the Emergency Surgery Score (ESS)
Authors: Raffee Liqaa , Almasarweh Sami A , Mazahreh Tagleb S , Alawneh Khaled , Alabdallah Nadeem Bilal , AL Hamoud Mohammad A , Aburayya Hamza A , Ayoub Fadi S , Issa Fadi , Ciottone Greg 
Published: November 2022

ObjectiveThe Emergency Surgery Score (ESS) is a predictive tool used to assess morbidity and mortality rates in patients undergoing emergent surgery. This study explores the ESS’s predictive ability and reliability in the Jordanian surgical population.DesignA retrospective validation study.SettingA tertiary hospital in Jordan.ParticipantsA database was created including patients who underwent emergent surgery in King Abdullah University Hospital from January 2017 to June 2021.Primary and secondary outcome measuresRelevant preoperative, intraoperative and postoperative variables were retrospectively and systematically gathered, and the ESS was calculated for each patient accordingly. In addition, a multivariable logistic regression analysis was performed to assess the correlations between the ESS and postoperative mortality and morbidity along with intensive care unit (ICU) admissions.ResultsOut of total of 1452 patients evaluated, 1322 patients were enrolled based on inclusion and exclusion criteria. The mean age of the population was 47.9 years old. 91.9% of the patients were admitted to the surgical ward through the emergency department, while the rest were referred from inpatient and outpatient facilities. The mortality and postoperative complication rates were 3.9% and 13.5%, respectively. Mortality rates increased as the ESS score gradually increased, and the ESS was evaluated as a strong predictor with a c-statistic value of 0.842 (95% CI 0.743 to 0.896). The postoperative complication and ICU admission rate also increased with reciprocal rises in the ESS. They were also evaluated as accurate predictors with a c-statistic value of 0.724 (95% CI 0.682 to 0.765) and a c-statistic value of 0.825 (95% CI 0.784 to 0.866), respectively.ConclusionThe ESS is a robust, accurate predictor of postoperative mortality and morbidity of emergency general surgery patients. Furthermore, it is an all-important tool to enhance emergency general surgery practices, in terms of mitigating risk, quality of care measures and patient counselling.