bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain
Authors: Rojas-Pérez Lilia C. , Narváez-Rincón Paulo C. , Rocha M. Angélica M. , Coelho Elisabete , Coimbra Manuel A. 
Published: October 2022

AbstractXylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis is an important step in a biorefinery context. This research evaluated the synergism among four classes of hydrolytic purified enzymes—endo-1,4-β-xylanase, α-l-arabinofuranosidase, β-xylosidase, and α-d-glucuronidase—over hydrolysis of glucuronoarabinoxylan (GAX) obtained from brewers’ spent grain (BSG) after alkaline extraction and ethanol precipitation. First, monosaccharides, uronic acids and glycosidic-linkages of alkaline extracted GAX fraction from BSG were characterized, after that different strategies based on the addition of one or two families of enzymes—endo-1,4-β-xylanase (GH10 and GH11) and α-l-arabinofuranosidase (GH43 and GH51)—cooperating with one β-xylosidase (GH43) and one α-d-glucuronidase (GH67) into enzymatic hydrolysis were assessed to obtain the best yield of xylose. The xylose release was monitored over time in the first 90 min and after a prolonged reaction up to 48 h of reaction. The highest yield of xylose was 63.6% (48 h, 40 ℃, pH 5.5), using a mixture of all enzymes devoid of α-l-arabinofuranosidase (GH43) family. These results highlight the importance of GH51 arabinofuranosidase debranching enzyme to allow a higher cleavage of the xylan backbone of GAX from BSG and their synergy with 2 endo-1,4-β-xylanase (GH10 and GH11), one β-xylosidase (GH43) and the inclusion of one α-d-glucuronidase (GH67) in the reaction system. Therefore, this study provides an environmentally friendly process to produce xylose from BSG through utilization of enzymes as catalysts.
Graphical Abstract
Shared decision-making and planning end-of-life care for patients with end-stage kidney disease: a protocol for developing and testing a complex intervention
Authors: Buur Louise Engelbrecht , Finderup Jeanette , Søndergaard Henning , Kannegaard Michell , Madsen Jens Kristian , Bekker Hilary Louise 
Published: October 2022

Abstract
Background
Internationally, it has been stressed that advance care planning integrated within kidney services can lead to more patients being involved in decisions for end-of-life care. In Denmark, there is no systematic approach to advance care planning and end-of-life care interventions within kidney services. A shared decision-making intervention for planning end-of-life care may support more effective treatment management between patients with end-stage kidney disease, their relatives and the health professionals. The purpose of this research is to find evidence to design a shared decision-making intervention and test its acceptability to patients with end-stage kidney disease, their relatives, and health professionals in Danish kidney services.

Methods
This research project will be conducted from November 2020 to November 2023 and is structured according to the UK Medical Research Council framework for complex intervention design and evaluation research. The development phase research includes mixed method surveys. First, a systematic literature review synthesising primary empirical evidence of patient-involvement interventions for patients with end-stage kidney disease making end-of-life care decisions will be conducted. Second, interview methods will be carried out with patients with end-stage kidney disease, relatives, and health professionals to identify experiences of involvement in decision-making and decisional needs when planning end-of-life care. Findings will inform the co-design of the shared decision-making intervention using an iterative process with our multiple-stakeholder steering committee. A pilot test across five kidney units assessing if the shared decision-making intervention is acceptable and feasible to patients, relatives, and health professionals providing services to support delivery of care in kidney services.

Discussion
This research will provide evidence informing the content and design of a shared decision-making intervention supporting patient-professional planning of end-of-life care for patients with end-stage kidney disease, and assessing its acceptability and feasibility when integrated within Danish kidney units. This research is the first step to innovating the involvement of patients in end-of-life care planning with kidney professionals.
Disarrangement and reorganization of the hippocampal functional connectivity during the spatial path adjustment of pigeons
Authors: Li Mengmeng , Cheng Shuguan , Fan Jiantao , Shang Zhigang , Wan Hong , Yang Lifang , Yang Long 
Published: October 2022

Abstract
Background
The hippocampus plays an important role to support path planning and adjustment in goal-directed spatial navigation. While we still only have limited knowledge about how do the hippocampal neural activities, especially the functional connectivity patterns, change during the spatial path adjustment. In this study, we measured the behavioural indicators and local field potentials of the pigeon (Columba livia, male and female) during a goal-directed navigational task with the detour paradigm, exploring the changing patterns of the hippocampal functional network connectivity of the bird during the spatial path learning and adjustment.

Results
Our study demonstrates that the pigeons progressively learned to solve the path adjustment task after the preferred path is blocked suddenly. Behavioural results show that both the total duration and the path lengths pigeons completed the task during the phase of adjustment are significantly longer than those during the acquisition and recovery phases. Furthermore, neural results show that hippocampal functional connectivity selectively changed during path adjustment. Specifically, we identified depressed connectivity in lower bands (delta and theta) and elevated connectivity in higher bands (slow-gamma and fast-gamma).

Conclusions
These results feature both the behavioural response and neural representation of the avian spatial cognitive learning process, suggesting that the functional disarrangement and reorganization of the connectivity in the avian hippocampus during different phases may contribute to our further understanding of the potential mechanism of path learning and adjustment.
Detecting a familiar person behind the surgical mask: recognition without identification among masked versus sunglasses-covered faces
Authors: Carlaw Brooke N. , Huebert Andrew M. , McNeely-White Katherine L. , Rhodes Matthew G. , Cleary Anne M. 
Published: October 2022

AbstractPrevious research has shown that even when famous people’s identities cannot be discerned from faces that have been filtered with monochromatic noise, these unidentifiable famous faces still tend to receive higher familiarity ratings than similarly filtered non-famous faces. Experiment 1 investigated whether a similar face recognition without identification effect would occur among faces whose identification was hindered through the wearing of a surgical mask. Among a mixture of famous and non-famous faces wearing surgical masks and hoods, participants rated how familiar each person seemed then attempted to identify the person. Though surgical masks significantly impaired identification of the famous faces, unidentified masked famous faces received higher familiarity ratings on average than the non-famous masked faces, suggesting that a sense of familiarity could still occur even when identification was impaired by the mask. Experiment 2 compared faces covered by surgical masks with faces covered by sunglasses. Though sunglasses impaired face identification more than surgical masks, the magnitude of the face recognition without identification effect was the same in both cases. This pattern suggests that holistic face processing is not a requirement for the sense of familiarity with a face, and that different facial feature types can contribute.
Comparison of pre-extubation propofol and midazolam to prevent emergence agitation in children—a prospective randomized study
Authors: Holyachi Renuka , Pratibha S. D. , Kagalkar Nirmala Devi , Karigar Shivanand L. , Gowni Geetha , Patil Vidya 
Published: October 2022

Abstract
Background
Emergence delirium is one of the most common and troublesome complications seen after inhalational anesthesia, with an estimated 20–80% incidence rate. It can lead to an increase in mortality, morbidity, and hospital stay, which indirectly reflects a major economic burden. The aim of our study is to compare the effect of propofol and two different doses of midazolam to prevent emergence agitation in children given 5 min before extubation. Seventy-five ASA (American Society of Anesthesiologists) Physical Status I–II patients aged 2–14 years who were scheduled for elective surgeries under general anesthesia were included in the study. Patients in the study groups were randomized into three groups 25 in each group; group P (propofol of 0.5 mg/kg iv), group ML (midazolam low dose of 0.03 mg/kg iv), and group M (midazolam of 0.05 mg/kg iv) given 5 min before extubation. The primary objective was to study the effect of propofol and two different doses of midazolam for the prevention of emergence agitation in children. The secondary objective was to observe the complications like bronchospasm, laryngospasm, persistent cough, and desaturation in pediatric patients.

Results
The time taken for emergence from anesthesia after surgery in the propofol group was 5.11 ± 1.223 min and in the group midazolam low dose and midazolam time taken was 8.53 ± 2.326 min and 12.45 ± 2.145 min, respectively, and was found to be statistically significant. The incidence of delirium observed with a Cole score of >3 was seen in 14 (56%) patients in group P, 7 (28%), and 6 (24%) patients of groups ML and M, respectively.

Conclusions
Midazolam in low doses given before extubation is effective in preventing the emergence delirium during the postoperative period in pediatric patients without delay in recovery during general anesthesia.
Does an Intradermal Vaccination for Monkeypox Make Sense?
Authors: Mrsny Randall J. 
Published: October 2022

AbstractMankind has recently had to deal a series of virus-mediated pandemics, resulting in extensive morbidity and mortality rates that have severely strained healthcare systems. While dealing with viral infections as a healthcare concern is not new, our exceptionally mobile society has added to the critical challenge of limiting pathogen spread of a highly transmissible virus prior to the generation, testing, and distribution of safe and effective vaccines. The tremendous global effort put forth to address the recent pandemic induced by SARS-CoV-2 infection has highlighted many of the strengths and weaknesses of how vaccines are identified, tested, and used to provide protection. These uncertainties are exacerbated by the lack of clear and consistent messaging that can occur when the processes of research, development, and clinical testing that normally requires years of study and consideration are compressed into a few months. In this commentary, I will provide some background on the intramuscular (IM), subcutaneous (SC), and intradermal (ID) administration routes used for injectable vaccines and some information on potential immunological outcomes. With this background, I will address the recent FDA decision to allow an approved vaccine against monkeypox virus to be administered by ID, as well as its initial approval route via SC, injection as a dose-sparing strategy to maximize immunization numbers using current stockpiles.
In Vitro Permeation Studies on Carvedilol Containing Dissolving Microarray Patches Quantified Using a Rapid and Simple HPLC-UV Analytical Method
Authors: Anjani Qonita Kurnia , Sabri Akmal Hidayat Bin , McGuckin Mary B. , Li Huanhuan , Hamid Khuriah Abdul , Donnelly Ryan F. 
Published: October 2022

AbstractAnalytical method validation is a vital element of drug formulation and delivery studies. Here, high-performance liquid chromatography in conjunction with UV detection (HPLC-UV) has been used to produce a straightforward, quick, yet sensitive analytical approach to quantify carvedilol (CAR). A C18 column was used to isolate the analyte from the mixture by isocratic elution with a mobile phase comprising a mixture of 0.1% v/v trifluoroacetic acid in water and acetonitrile in a ratio of 65:35 v/v at a flow rate of 0.6 mL min−1. Linearity was observed for CAR concentrations within the range of 1.5–50 μg mL−1 (R2 = 0.999) in phosphate buffer saline and within the range of 0.2–6.2 μg mL−1 (R2 = 0.9999) in methanol. The International Council on Harmonization (ICH) requirements were followed throughout the validation of the isocratic approach, rendering it specific, accurate, and precise. Moreover, robustness tests indicated that the method remained selective and specific despite small deliberate changes to environmental and operational factors. An efficient extraction procedure was also developed to extract and quantify CAR from excised neonatal porcine skin, resulting in recovery rates ranging from 95 to 97%. The methods reported here have been successfully utilised to evaluate CAR permeation, both transdermally and intradermally following application of a dissolving microarray patch (MAP) to excised neonatal porcine skin.
Graphical Abstract
Formal Methylene Insertion into the C–H Bond of α-Carbonyl Aldonitrones with Dimethylsulfoxonium Methylide
Authors: Sakurai Takanobu , Yano Tohru , Suga Takuya , Soeta Takahiro , Ukaji Yutaka 
Published: October 2022

Abstract
A methylene group was introduced into the C–H bond of α-carbonyl aldonitrones by reaction with dimethylsulfoxonium methylide, producing one-carbon homologated C-methyl ketonitrones. This formal methylene insertion was applied to one-pot synthesis of quaternary C3-methyl isoxazolidines via successive 1,3-dipolar cycloaddition with alkenes bearing an electron withdrawing group.
Treating Waste with Waste: Facile Preparation of Elastic Thermoplastic Polyurethane Monolith for Efficient Oil/Water Separation
Authors: Wang Qidong , Yu Fangjun , Zhu Jiayi , Li Nan , Zhang Ye , Peng Xiangwen , Shang Jizhen , Li Shuai , Wang Hua , Uyama Hiroshi 
Published: October 2022

Abstract
An auspicious thermoplastic polyurethane (TPU) monolith has been prepared by a facile and cost-effective method of thermally induced phase separation (TIPS). With superb porous structure, excellent hydrophobicity/oleophilicity, and superior elasticity, the obtained TPU monolith exhibits an outstanding adsorption capacity, high oil/water separation efficiency, and good recycling performance, achieving the goal of treating waste with waste.
The Arizona Sexual Experiences Scale-the Thai Translation (ASEX-Thai): Reliability and validity in Thai patients with mental disorders
Authors: Charoenmakpol Natthaphon , Chiddaycha Mayteewat , Wainipitapong Sorawit 
Published: October 2022

Background: Sexual dysfunction is common among patients with mental disorders but receives less clinical attention, especially in Thailand and other Asian countries. The Arizona Sexual Experiences Scale-the Thai Translation (ASEX-Thai), a self-rated, brief, questionnaire is a potential tool for screening for sexual dysfunction in this population. Our study aimed to assess the reliability and validity of ASEX-Thai in Thai patients with mental disorders. Methods: We enrolled 202 patients from an outpatient psychiatric department at a tertiary hospital in Bangkok, Thailand. Demographic data, clinical data, and diagnosis of sexual dysfunction were assessed. ASEX-Thai was done, and we analyzed the test’s psychometric properties. Results: Most participants were diagnosed with major depressive disorder (48%). There was a positive correlation between the ASEX-Thai and sexual dysfunction diagnosis (r = 0.402, p < 0.001). The KMO coefficient was 0.77 and Barlett’s sphericity test was significant (χ 2 = 409.76, p<0.001). A score of ≥ 17 points of the ASEX-Thai was the most suitable for sexual dysfunction screening (sensitivity 77.23 %, and specificity 58.42 %). For reliability, the Cronbach’s alpha coefficient (0.831) showed good internal consistency. Conclusions: The ASEX-Thai is a valid and reliable self-rated questionnaire for screening for sexual dysfunction among Thai patients with mental disorders. The test could help clinicians to evaluate this undetected condition and deliver proper interventions.
Assessing the impacts of conservation volunteering on participant wellbeing: a systematic review protocol
Authors: Nuuttila Hanna 
Published: October 2022

Background: Volunteers and citizen scientists have become an essential element of most nature conservation and restoration activities due to lack of resources but also due to the wish to engage and interact with local communities where conservation activities take place. Environmental or conservation volunteering is also considered to be a key resource in achieving much needed, ambitious nature restoration programs. Practical conservation work and various forms of environmental enhancement along with recreational and therapeutic use of natural or green and blue spaces have been studied for some time. The value of volunteers and the work is widely acknowledged but few studies have been carried out on the impacts of participating on the volunteers themselves. Using this protocol, a study will be undertaken to assess how impacts of participation have been assessed and reported in the literature; what these reported impacts are; how these are related to reported barriers and motivations for volunteering and whether they are affected by the region or country of study. Methods: This paper will identify studies that have described and assessed impacts of conservation and restoration volunteering on participants at an individual level, with a specific focus on physical, mental or societal wellbeing of individuals. Representative studies were sought from major search engines and relevant stakeholder publications, including both peer-reviewed and ‘grey literature’ in predominantly English language publications, published between 2000 and 2020. A priori inclusion criteria consisted of those publications and reports on studies with volunteer and community participants and which described impacts of, motivations for and barriers to participation. After a critical appraisal, a total of 105 articles were selected for further analysis to provide a narrative and mixed methods synthesis of the evidence base.
Sustainability of the regional financial system: a case study of the Northwestern Federal District
Authors: Evgenievich Barykin Sergey , Aleksandrovich Mikheev Alexey , Grigorievna Kiseleva Elena , Evgenievich Putikhin Yuriy , De La Poza Plaza Elena , Sergeevna Alekseeva Natalia 
Published: October 2022

Background: This article provides an assessment of the sustainability of Russian regions’ financial systems. The study is based on the methods of generalization and synthesis, correlation-regression analysis, and multivariate classification. Since the structure of the regional financial system is complex, several works are devoted to studying its sustainability issues. The relevance of the study topic is confirmed by the lack of a systematic approach to assessing the integral index of sustainability and the possibility of using various tools in determining the complex indicator. Methods: This methodology with application of mathematical statistics methods makes it possible to assess the financial system sustainability in four sectors, to include the leading indicators in the assessment, and to identify regions with extreme values of debt burden indicators. The method was tested for the regions of the Northwestern Federal District (NWFD) for the period 2010 - 2019 to classify the regions according to three levels of debt sustainability. Data collection from the 1st January to 30th April 2022 included statistical data from government open internet sources, sectors studied relate to government, and municipal budgets in the NWFD. Authors analyzed regional debt sustainability indicators and identified themes in the field of sustainability studies for the NWFD. Results: An increased level of financial system sustainability was observed among the NWFD regions in the corporative and personal finance sectors, indicating a significant contribution of businesses and households to maintaining the balance and sustainability of the financial system in Russia as a whole. The results of the study also identified that the NWFD regions belong to three clusters: cluster 1 - high debt sustainability; cluster 2 - medium debt sustainability; and cluster 3 - low debt sustainability. Conclusions: The study results allowed the identification of regions with a constantly high level of debt, financial, and corporative sustainability.
The needle percentage in the leafy shoot biomass of Scots pine in climate gradients of Eurasia
Authors: Usoltsev Vladimir , Zukow Walery , Tsepordey Ivan 
Published: October 2022

The efficiency of using photosynthetically active radiation by trees largely depends on the architecture of the assimilation apparatus in their crown. It is known that an increase in the level of atmospheric pollution is accompanied, on the one hand, by an increase in the foliage density on the leafy shoot length, and on the other hand, by an increase in the transparency of the crown due to the expansion of the leafless inner part of the crown. On this basis, we assume that the foliage percentage of leafy shoots (PL) is a sensitive indicator of changes in growing conditions not only due to environmental pollution, but also in broader ecological aspects, in particular, in the geographical or climatic gradients of Eurasia. For this purpose, a database of empirical data has been formed in the amount of 558 model trees of Scots pine of natural and artificial origin from 6 regions of Northern Eurasia with a measured percentage of needle in the leafy shoot biomass. A regression model has been designed, in which the contributions of mensuration variables (tree age and stem diameter), climatic variables (temperature and precipitation) and the origin of stands (natural and artificial) to explain the variability of the desired PL indicator are 15, 53 and 30 %, respectively. It is concluded that with a decrease in annual precipitation from 600 to 300 mm, there is an increase in PL by 0.2..0.3% for every 10 mm of annual precipitation, both in natural stands and in plantations. When the January temperature decreases from 0°C to -20 °C, PL increases by 0.8...1.0% for each 1°C. Thus, the foliage percentage of leafy shoots of Scots pine is an indicator of changes in growing conditions in the climate gradients of Eurasia.
A RECEPÇÃO DE E.T.A. HOFFMANN NA FRANÇA
Authors: Batalha Maria Cristina 
Published: October 2022

A descoberta de E.T.A. Hoffmann provocou uma fermentação entre os franceses nos anos de 1828 até mais ou menos 1840. A partir das traduções francesas de contos do autor alemão um debate se desencadeia através da imprensa, despertando interesse pelo novo modelo literário, vulgarizando o termo "fantástico" e servindo de emulação para diversos autores. Seus contos trazem um fermento novo para o romantismo francês. A difusão das obras traduzidas de Hoffmann ultrapassa os limites de um acontecimento literário para esboçar a trajetória de um mito construído por seus divulgadores e pelos artistas que nele vão buscar sua inspiração.