bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGARUH MODEL PEMBELAJARAN MINDMAPPING TERHADAP HASIL BELAJAR BIOLOGI SISWA DI KELAS VIII SMPN 8 SUNGAI PENUH
Authors: Adelia Ismi , Tiara Tiara , Husmarlina Husmarlina 
Published: July 2022

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh model pembelajaran Mind mapping terhadap hasil belajar siswa. Penelitian ini merupakan penelitian eksperimen tipe Pre-Experimental Design dengan desain One-Group Pretest-Posttest Design. Populasi penelitian ini adalah seluruh siswa kelas VIII SMP Negeri 8 Sungai Penuh yang terdiri dari 7 kelas. Sampel penelitian ditentukan secara acak, yaitu siswa kelas VIII A yang terdiri atas 35 siswa. Data dianalisis dengan menggunaan uji Wilcoxon. Hasil penelitian menunjukkan bahwa terdapat perbedaan rata-rata hasil belajar siswa sebelum dan sesudah menggunakan model pembelajaran Mindmapping dengan taraf selisih rata-rata pretest dan posttest adalah 14,00. Berdasarkan Hasil uji wilcoxon diketahui bahwa nilai signifikasi 0,00 < 0,05 yang mengindikasikan model pembelajaran Mind Mapping berpengaruh terhadap hasil belajar siswa. Kata kunci : Model Pembelajaran, Mind Mapping, Hasil Belajar ABSTRACT The study was conducted to determine the effect of the Mind Mapping learning model on the learning outcomes of eighth-grade students. This study was an experiment by Pre Experimental Design with One-Group Pretest-Posttest Design. The population was all grade eight students of SMPN 8 Sungai Penuh. The sample was determined using the randomized sample technique, which samples were class VIII-A. Data were analyzed using the Wilcoxon test. The results showed that there was a difference in the average student learning outcomes before and after using the mind mapping learning model with the average difference between pretest and posttest was 14.00. Based on the Wilcoxon test results, it is known that the significance value is 0.00 < 0.05, which indicates that the Mind Mapping learning model affects student learning outcomes. Keywords : Learning Model, Mind Mapping, Learning outcomes
PENERAPAN MODEL ADDIE DALAM PENGEMBANGAN MULTIMEDIA PEMBELAJARAN BERBASIS LITERASI SAINS
Authors: Latip Abdul 
Published: July 2022

ABSTRAK Pada era perkembangan teknologi dan ilmu pengetahuan, multimedia memegang peran dalam mengakomodasi pembelajaran IPA yang lebih bermakna melalui visualisasi konsep yang abstrrak dan kontektualisasi konsep dalam kehidupan sehari-hari. Penelitian ini bertujuan menganalisis dan menggambarkan tahapan pengembangan multimedia pembelajaran berbasis literasi sains dengan model ADDIE. Metode penelitian yang digunakan dalam penelitian ini adalah deskriptif kualitatif yang menjabarkan penerapan model ADDIE dalam pengembangan multimedia pembelajaran berbasis literasi sains. Hasil penelitian yang dilakukan diperoleh gambaran sebagai berikut: 1) Analysis terdiri dari analisis permasalahan, konten, kurikulum dan objek multimedia. 2) Design terdiri dari proses analisis wacana, pembuatan flowchart dan story board, 3) Develpoment merupakan tahap pembuatan multimedia dan validasi multimedia oleh tim ahli serta tim guru, 4) Implementation merupakan tahap uji coba terbatas dalam pembelajaran IPA di Kelas, dan 5) Evaluation merupakan tahap pemberian umpan balik terhadap multimedia pembelajaran berbasis literasi sains yang sudah dikembangkan oleh alhli, guru dan siswa. Berdasarkan penerapan tahapan model ADDIE yang dilakukan diperoleh gambaran bahwa model tersebut dapat digunakan untuk pengembangan multimedia pembelajaran IPA dengan basis pengembangan berupa literasi sains. Kata Kunci: Literasi Sains, Model ADDIE, Multimedia ABSTRACT In the era of technological and scientific development, multimedia plays a role in accommodating more meaningful science learning through visualization of abstract concepts and contextualization of concepts in everyday life. This study aims to analyze and describe the stages of developing scientific literacy-based learning multimedia using the ADDIE model. The research method used in this research is descriptive qualitative which describes the application of the ADDIE model in the development of scientific literacy-based learning multimedia. The results of the research carried out are described as follows: 1) The analysis consists of analysis of problems, content, curriculum and multimedia objects. 2) Design consists of a discourse analysis process, making flowcharts and story boards, 3) Development is the stage of making multimedia and multimedia validation by a team of experts and a team of teachers, 4) Implementation is a limited trial stage in learning science in class, and 5) Evaluation is the stage of providing feedback to multimedia learning based on scientific literacy that has been developed by experts, teachers and students. Based on the implementation of the stages of the ADDIE model carried out, it is obtained an illustration that the model can be used for the development of multimedia learning in science with the basis of development in the form of scientific literacy. Keyword: Scientific Literacy, ADDIE model, Multimedia
PENGEMBANGAN E-MODUL BERORIENTASI MODEL CURIOUS NOTE PROGRAM UNTUK MELATIHKAN KETERAMPILAN BERPIKIR KRITIS SISWA SMA PADA MATERI GELOMBANG
Authors: Kartika Anggun Citra , Sutarno Sutarno , Purwanto Andik 
Published: July 2022

ABSTRAK Penelitian ini merupakan penelitian dan pengembangan (R&D) yang bertujuan untuk menghasilkan produk e-modul berorientasi Model Curious Note Program untuk melatihkan keterampilan berpikir kritis siswa pada materi gelombang. Model pengembangan yang digunakan pada penelitian ini adalah model pengembangan 4D yang dilakukan terbatas pada tahap 3D, yaitu define, design, dan development. Tahap define terdiri atas kegiatan merancang instrumen untuk tahap define, analisis RPP, review dokumen modul yang digunakan di sekolah dan analisis kebutuhan pengembangan. Tahap design terdiri atas rancangan e-modul dan rancangan instrumen untuk mengevaluasi e-modul. Tahap development terdiri atas validasi ahli dan uji keterbacaan. Berdasarkan hasil validasi ahli, diperoleh persentase skor keseluruhan sebesar 93,48% dengan kategori sangat layak. Berdasarkan hasil uji keterbacaan produk, diperoleh persentase skor keseluruhan sebesar 92% dengan kategori sangat layak. Berdasarkan hasil validasi dan uji keterbacaan yang telah dilakukan oleh ahli dapat disimpulkan bahwa e-modul yang dikembangkan “sangat layak” untuk dilanjutkan pada uji coba lapangan. Kata kunci: Penelitian dan Pengembangan, E-modul, Curious Note Program, Keterampilan Berpikir Kritis, Flip PDF ProfesionalABSTRACTresearch is a research and development (R&D) that aims to produce an e-module product oriented to the Curious Note Program model to train students' critical thinking skills on wave material. The development model used in this study is a 4D development model which is limited to the 3D stage, namely define, design, and development. The define stage consists of designing instruments for the define stage, analyzing lesson plans, reviewing module documents used in schools and analyzing development needs. The design stage consists of designing e-modules and designing instruments to evaluate e-modules. The development stage consists of expert validation and readability testing. Based on the results of expert validation, the percentage of an overall average score of 93,48% with a very feasible category. Based on the results of the product readability test, it was found that the average score percentage the percentage of an overall average score of 92% with a very feasible category Based on the results of the validation and legibility tests that have been carried out by the experts, it can be concluded that the e-modules that has been developed is "very feasible" to be used. Keywords: Research and Development, E-modules, Curious Note Program, Critical Thinking Skills, Flip PDF Profesional
Analisis Morfometrik-Biofisika antara Sapi Karapan, Sapi Sonok dan Sapi Peternak Madura (Bos primiginius) di Kabupaten Sumenep
Authors: Miftahussurur Mohammad , Zayadi Hasan , Tito Sama' Iradat 
Published: July 2022

The purpose of this study was to determine the morphometric and biophysical differences between three types of Madura Cattle, namely Karapan Cattle, Sonok Cattle and Breeder Cattle. The method used is purposive sampling by looking for certain material criteria. The materials used were 7 Karapan Cows, 7 Sonok Cows and 7 Breeder Cows. There are 20 variable measurements in morphometric data collection and obtained 10 variables that have significant differences. This difference is caused by the herbal medicine and the treatment of each cow. In the biophysics of the Karapan Cow, which is to compare the speed and acceleration of the seven Karapan Cows. The results of biophysics on Karapan Cattle show that it has an average speed of 5 m/s. While the superior Karapan Cow was occupied by the 4th Karapan Cow with an acceleration of 1.18 m/s². In biophysics, the Sonok Cow itself has a speed below 0 m/s. Therefore the resulting acceleration is only recorded in seconds. This of course is returned to the function of the Sonok Cow which is only judged on the tame and beauty of its body. Keywords: Morphometric, Biophysical, Karapan Cow, Sonok Cow, Madura Cattle BreedersABSTRAKTujuan penelitian ini adalah untuk mengetahui perbedaan morfometrik dan biofisika antara tiga jenis Sapi Madura yaitu Sapi Karapan, Sapi Sonok dan Sapi Peternak. Metode yang digunakan adalah purposive sampling dengan mencari kriteria-kriteria materi tertentu. Materi yang digunakan adalah 7 ekor Sapi Karapan, 7 ekor Sapi Sonok dan 7 ekor Sapi Peternak. Terdapat 20 pengukuran variabel dalam pengambilan data morfometrik dan didapatkan 10 variabel yang memiliki perbedaan secara nyata. Perbedaan ini disebabkan oleh faktor jamu dan perlakuan terhadap masing-masing Sapi. Pada biofisika Sapi Karapan yaitu membandingkan kecepatan dan percepatan dari ketujuh Sapi Karapan ini. Hasil biofiska pada Sapi Karapan menunjukkan memiliki rata-rata kecepatan 5 m/s. Sedangkan Sapi Karapan terunggul diduduki oleh Sapi Karapan ke-4 dengan percepatan 1,18 m/s². Pada biofisika Sapi Sonok sendiri memiliki kecepatan dibawah 0 m/s. Oleh karena itu percepatan yang dihasilkan hanya tercatat dalam hitungan detik. Hal ini tentunya dikembalikan lagi kepada fungsi Sapi Sonok yang hanya dinilai pada kejinakan dan kecantikan tubuhnya.Kata kunci : Morfometrik, Biofisika, Sapi Karapan, Sapi Sonok, Sapi Peternak
Clinical relevance of molecular testing methods in the diagnosis and guidance of therapy in patients with staphylococcal empyema: a systematic review and meta-analysis
Authors: Ojha Suvash Chandra , Chen Ke , Yuan Yue , Ahmed Sarfraz , Malik Aijaz Ahmad , Nisha Mehru , Sheng Yun-Jian , Sun Changfeng , Wu Gang , Deng Cun-Liang 
Published: July 2022

BackgroundEfficient detection tools for determining staphylococcal pleural infection are critical for its eradication. The objective of this meta-analysis was to assess the diagnostic utility of nucleic acid amplification tests (NAAT) in suspected empyema cases to identify staphylococcal strains and avoid unnecessary empiric methicillin-resistant Staphylococcus aureus (MRSA) therapy.MethodsFrom inception to July 24, 2021, relevant records were retrieved from PubMed, Embase, Scopus, Web of Science, and the Cochrane Library. The quality of studies was determined using the QUADAS-2 tool. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and hierarchical summary receiver operating characteristic (HSROC) curve for NAAT’s diagnostic performance were evaluated using an HSROC model.ResultsEight studies comprising 424 samples evaluated NAAT accuracy for Staphylococcus aureus (SA) identification, while four studies comprising 317 samples evaluated methicillin-resistant Staphylococcus aureus (MRSA) identification. The pooled NAAT summary estimates for detection of both SA (sensitivity: 0.35 (95% CI 0.19–0.55), specificity: 0.95 (95% CI 0.92–0.97), PLR: 7.92 (95% CI 4.98–12.59), NLR: 0.44 (95% CI 0.14–1.46), and DOR: 24.0 (95% CI 6.59–87.61) ) and MRSA (sensitivity: 0.45 (95% CI 0.15–0.78), specificity: 0.93 (95% CI 0.89–0.95), PLR: 10.06 (95% CI 1.49–67.69), NLR: 0.69 (95% CI 0.41–1.15), and DOR: 27.18 (95% CI 2.97–248.6) ) were comparable. The I2 statistical scores for MRSA and SA identification sensitivity were 13.7% and 74.9%, respectively, indicating mild to substantial heterogeneity. PCR was frequently used among NAA tests, and its diagnostic accuracy coincided well with the overall summary estimates. A meta-regression and subgroup analysis of country, setting, study design, patient selection, and sample condition could not explain the heterogeneity (meta-regression P = 0.66, P = 0.46, P = 0.98, P = 0.68, and P = 0.79, respectively) in diagnostic effectiveness.ConclusionsOur study suggested that the diagnostic accuracy of NAA tests is currently inadequate to substitute culture as a principal screening test. NAAT could be used in conjunction with microbiological culture due to the advantage of faster results and in situations where culture tests are not doable.
Recombinant polymerase amplification combined with lateral flow strips for the detection of deep-seated Candida krusei infections
Authors: Zhao Mengdi , Wang Xizhen , Wang Kun , Li Yuanyuan , Wang Yan , Zhou Ping , Wang Lei , Zhu Wenjun 
Published: July 2022

The incidence of Candida infections in intensive care units (ICU) has significantly increased in recent years, and these infections have become one of the most serious complications threatening the lives of ICU patients. The proportion of non-Candida albicans infections, such as Candida krusei and Candida glabrata infections, which are resistant to fluconazole, is increasing each year. Early identification of the strains causing Candida infections is important for the timely implementation of targeted treatments to save patients’ lives. However, the current methods of direct microscopy, culture, and histopathology, as well as other diagnostic methods, have many shortcomings, such as their low sensitivity and long assay times; therefore, they cannot meet the needs for early clinical diagnosis. Recombinant polymerase amplification (RPA) is a promising isothermal amplification technique that can be performed without sophisticated instruments and equipment, and is suitable for use in resource-poor areas. RPA combined with lateral flow strips (LFS) can be used to rapidly amplify and visualize target genes within 20 min. In this study, RPA-LFS was used to amplify the internal transcribed spacer 2 (ITS2) region of C. krusei. The primer-probe design was optimized by introduction of base mismatches (probe modification of five bases) to obtain a specific and sensitive primer-probe combination for the detection of clinical specimens. Thirty-five common clinical pathogens were tested with RPA-LFS to determine the specificity of the detection system. The RPA-LFS system specifically detected C. krusei without cross-reaction with other fungi or bacteria. A gradient dilution of the template was tested to explore the lower limit of detection and sensitivity of the assay. The sensitivity was 10 CFU/50 µL per reaction, without interference from genomic DNA of other species. The RPA-LFS and qPCR assays were performed on 189 clinical specimens to evaluate the detection performance of the RPA-LFS system. Seventy-six specimens were identified as C. krusei, indicating a detection rate of 40.2%. The results were consistent with those of qPCR and conventional culture methods. The RPA-LFS system established in our study provides a reliable molecular diagnostic method for the detection of C. krusei, thus meeting the urgent need for rapid, specific, sensitive, and portable clinical field testing.
Reproductive Outcomes of In Vitro Fertilization and Fresh Embryo Transfer in Infertile Women With Adenomyosis: A Retrospective Cohort Study
Authors: Liang Tingting , Zhang Wen , Pan Ningning , Han Bing , Li Rong , Ma Caihong 
Published: July 2022

BackgroundAdenomyosis is commonly encountered in infertile women; however, it is still unclear whether adenomyosis has a detrimental effect on in vitro fertilization and embryo transfer (IVF-ET) outcomes.MethodWe enrolled 1146 patients with adenomyosis and 1146 frequency-matched control women in a 1:1 ratio based on age, BMI, and basal follicle-stimulating hormone (FSH) level. After controlling for other factors, the rates of clinical pregnancy, miscarriage, live birth, and obstetric complications were compared between two groups.ResultsThere was no significant difference in clinical pregnancy rate between the two groups (38.1% vs. 41.6%; P=0.088). The implantation rate (25.6% versus 28.6%, P=0.027) and live birth rate (26% versus 31.5%, P=0.004) were significantly lower in the women with adenomyosis than in the controls. The miscarriage rate in the adenomyosis group was higher than that in the control group (29.1% versus 17.2%, P=0.001). After adjusting for confounding factors, multivariate analysis showed the clinical pregnancy rate was not statistically different between the two groups (OR: 0.852, P=0.070). In the adenomyosis group, the rate of miscarriage(OR: 1.877, P=0.000), placenta previa (OR: 2.996, P=0.042)and preeclampsia (OR: 2.287, P=0.042)were increased significantly, while live birth rate (OR: 0.541, P=0.000) was reduced significantly than control group.ConclusionAdenomyosis has negative effect on IVF-ET outcomes in which miscarriage risk increased, live birth rate reduced and obstetric complications increased.
5-Hydroxymethylcytosine profiles in plasma cell-free DNA reflect molecular characteristics of diabetic kidney disease
Authors: Chu Jin-Lin , Bi Shu-Hong , He Yao , Ma Rui-Yao , Wan Xing-Yu , Wang Zi-Hao , Zhang Lei , Zheng Meng-Zhu , Yang Zhan-Qun , Du Ling-Wei , Maimaiti Yiminiguli , Biekedawulaiti Gulinazi , Duolikun Maimaitiyasen , Chen Hang-Yu , Chen Long , Li Lin-Lin , Tie Lu , Lin Jian 
Published: July 2022

BackgroundDiabetic kidney disease (DKD), one of the main complications of diabetes mellitus (DM), has become a frequent cause of end-stage renal disease. A clinically convenient, non-invasive approach for monitoring the development of DKD would benefit the overall life quality of patients with DM and contribute to lower medical burdens through promoting preventive interventions.MethodsWe utilized 5hmC-Seal to profile genome-wide 5-hydroxymethylcytosines in plasma cell-free DNA (cfDNA). Candidate genes were identified by intersecting the differentially hydroxymethylated genes and differentially expressed genes from the GSE30528 and GSE30529. Then, a protein interaction network was constructed for the candidate genes, and the hub genes were identified by the MCODE and cytoHubba algorithm. The correlation analysis between the hydroxymethylation level of the hub genes and estimated glomerular filtration rate (eGFR) was carried out. Finally, we demonstrated differences in expression levels of the protein was verified by constructing a mouse model of DKD. In addition, we constructed a network of interactions between drugs and hub genes using the Comparative Toxicogenomics Database.ResultsThis study found that there were significant differences in the overall distribution of 5hmC in plasma of patients with DKD, and an alteration of hydroxymethylation levels in genomic regions involved in inflammatory pathways which participate in the immune response. The final 5 hub genes, including (CTNNB1, MYD88, CD28, VCAM1, CD44) were confirmed. Further analysis indicated that this 5-gene signature showed a good capacity to distinguish between DKD and DM, and was found that protein levels were increased in renal tissue of DKD mice. Correlation analysis indicated that the hydroxymethylation level of 5 hub genes were nagatively correlated with eGFR. Toxicogenomics analysis showed that a variety of drugs for the treatment of DKD can reduce the expression levels of 4 hub genes (CD44, MYD88, VCAM1, CTNNB1).ConclusionsThe 5hmC-Seal assay was successfully applied to the plasma cfDNA samples from a cohort of DM patients with or without DKD. Altered 5hmC signatures indicate that 5hmC-Seal has the potential to be a non-invasive epigenetic tool for monitoring the development of DKD and it provides new insight for the future molecularly targeted anti-inflammation therapeutic strategies of DKD.
Liver fibrosis progression in a cohort of young HIV and HIV/ HBV co-infected patients: A longitudinal study using non-invasive APRI and Fib-4 scores
Authors: Iacob Diana Gabriela , Luminos Monica , Benea Otilia Elisabeta , Tudor Ana-Maria , Olariu Cristina Mihaela , Iacob Simona Alexandra , Ruta Simona 
Published: July 2022

BackgroundThe risk of liver fibrosis increases over time in HIV and HIV-HBV individuals even under antiretroviral treatment (ART), warranting a rigorous and periodic monitorization. Given the lower availability of transient elastography, we aimed to assess the longitudinal variation of two non-invasive liver fibrosis scores, APRI and Fib-4, in cases with HIV monoinfection, HIV-HBV co-infection and individuals with HBsAg-seroclearance.MethodsWe performed an observational retrospective study between 2013 and 2019 on 212 HIV patients including 111 individuals with HIV mono-infection, 62 individuals with HIV-HBV co-infection and positive HBsAg and 39 cases with HIV-HBV infection and HBsAg-loss. The groups were followed at 36, 48, and 60 months. Liver fibrosis was indicated by an APRI &gt;0.5 or Fib-4≥1.45 score and advanced fibrosis by an APRI score &gt;1.5 or Fib-4 &gt;3.25. Logistic regression with generalized estimating equations (GEE) was used to assess the predictors for the presence of liver fibrosis over time.ResultsDuring a median follow-up of 58.5 months the prevalence of liver fibrosis in all patients increased with 0.5% reaching 11.3% using an APRI score and with 0.9% reaching 10.8% using the Fib-4 score. At the visit corresponding to 60 months the prevalence of liver fibrosis was higher in all HIV-HBV patients compared with individuals with HIV mono-infection, namely: 16.1% on APRI and 12.9% on the Fib-4 score in HIV-HBV/HBsAg-positive individuals, 12.8% on both APRI and Fib-4 scores in HIV-HBV/HBsAg-negative individuals vs. 8.1 and 9%, respectively in HIV mono-infection. The presence of liver fibrosis over the study period was independently associated with plasma HIV RNA, CD4+T cell counts, HIV-HBV co-infection (for APRI &gt;0.5) and ART non-adherence (for Fib-4 &gt;1.45). At the final visit, non-adherence to ART and CD4+T cell counts remained associated with liver fibrosis.ConclusionsThe study found a slow progression of APRI and Fib-4 scores over time in young PLWH with extensive ART. Liver fibrosis scores continued to increase in patients with HIV mono-infection yet remained lower than in HIV-HBV patients irrespective on the presence of HBsAg. The periodic follow-up using non-invasive scores on the long-term could help improve the surveillance in low-income settings and high scores should be followed by additional diagnostic methods.