bluuebunny/crossref_metadata_2025_split huggingface dataset:
Jammed Micro‐Flake Hydrogel for Four‐Dimensional Living Cell Bioprinting
Authors: Ding Aixiang , Jeon Oju , Cleveland David , Gasvoda Kaelyn L. , Wells Derrick , Lee Sang Jin , Alsberg Eben 
Published: January 2022

Abstract4D bioprinting is promising to build cell‐laden constructs (bioconstructs) with complex geometries and functions for tissue/organ regeneration applications. The development of hydrogel‐based 4D bioinks, especially those allowing living cell printing, with easy preparation, defined composition, and controlled physical properties is critically important for 4D bioprinting. Here, a single‐component jammed micro‐flake hydrogel (MFH) system with heterogeneous size distribution, which differs from the conventional granular microgel, has been developed as a new cell‐laden bioink for 4D bioprinting. This jammed cytocompatible MFH features scalable production and straightforward composition with shear‐thinning, shear‐yielding, and rapid self‐healing properties. As such, it can be smoothly printed into stable 3D bioconstructs, which can be further cross‐linked to form a gradient in cross‐linking density when a photoinitiator and a UV absorber are incorporated. After being subject to shape morphing, a variety of complex bioconstructs with well‐defined configurations and high cell viability are obtained. Based on this system, 4D cartilage‐like tissue formation is demonstrated as a proof‐of‐concept. The establishment of this versatile new 4D bioink system may open up a number of applications in tissue engineering.
Modeling Human Thyroid Development by Fetal Tissue‐Derived Organoid Culture
Authors: Liang Jianqing , Qian Jun , Yang Li , Chen Xiaojun , Wang Xiaoning , Lin Xinhua , Wang Xiaoyue , Zhao Bing 
Published: January 2022

AbstractEuthyroidism is of profound importance for lifetime health. However, the early diagnosis or therapeutics of thyroid developmental defects has not been established, mainly due to limited understanding of human thyroid development and a lack of recapitulating research model. Herein, the authors elaborate the cell atlas and potential regulatory signaling of the evolution of heterogeneous thyrocyte population from 12 to 16 gestational weeks. Moreover, they establish a long‐term culture of human fetal thyroid organoids (hFTOs) system, which retains the fetal thyroid lineages and molecular signatures, as well as the ability to generate functional human thyroid follicles post mice renal transplantation. Notably, cAMP signaling activation in hFTOs by forskolin boosts the maturation of follicle and thus thyroid hormone T4 secretion, which recapitulates the key developmental events of fetal thyroid. Employing this ex vivo system, it is found that enhanced chromatin accessibility at thyroid maturation genes (such as TPO and TG) loci permits the transcription for hormone production. This study provides the cell atlas of and an organoid model for human thyroid development, which will facilitate thyroid research and prospective medicine.
Next‐Generation Cobalt‐Free Cathodes – A Prospective Solution to the Battery Industry's Cobalt Problem
Authors: Muralidharan Nitin , Self Ethan C. , Dixit Marm , Du Zhijia , Essehli Rachid , Amin Ruhul , Nanda Jagjit , Belharouak Ilias 
Published: January 2022

AbstractLithium‐ion batteries are overreliant on cobalt containing cathodes. Current projections estimate that hundreds of millions of electric vehicles (EVs) will be on the road by 2050, and this ever‐growing demand threatens to deplete global cobalt reserves at an alarming rate. Moreover, cobalt supply chain issues have significantly increased cobalt prices throughout the last decade. As such, energy storage research and development need to reduce the reliance on cobalt to meet ever‐growing demand for lithium‐ion batteries. The present review summarizes the science and technology gaps and potential of numerous cobalt‐free Li‐ion cathodes including layered, spinel, olivine, and disordered rock‐salt systems. Despite the promising performance of these Co‐free cathodes, scale‐up and manufacturing bottlenecks associated with these materials must also be addressed to enable widespread adoption in commercial batteries. Overall, this review broadly highlights the enormous promise of “zero‐cobalt” Li‐ion batteries to enable sustainable production of EVs in the coming decades.
Muscle Transcriptomics Shows Overexpression of Cadherin 1 in Inclusion Body Myositis
Authors: Ikenaga Chiseko , Date Hidetoshi , Kanagawa Motoi , Mitsui Jun , Ishiura Hiroyuki , Yoshimura Jun , Pinal‐Fernandez Iago , Mammen Andrew L. , Lloyd Thomas E. , Tsuji Shoji , Shimizu Jun , Toda Tatsushi , Goto Jun 
Published: January 2022

ObjectiveThis study aimed to elucidate the molecular features of inclusion body myositis (IBM).MethodsWe performed RNA sequencing analysis of muscle biopsy samples from 67 participants, consisting of 58 myositis patients with the pathological finding of CD8‐positive T cells invading non‐necrotic muscle fibers expressing major histocompatibility complex class I (43 IBM, 6 polymyositis, and 9 unclassifiable myositis), and 9 controls.ResultsCluster analysis, principal component analysis, and pathway analysis showed that differentially expressed genes and pathways identified in IBM and polymyositis were mostly comparable. However, pathways related to cell adhesion molecules were upregulated in IBM as compared with polymyositis and controls (p < 0.01). Notably, CDH1, which encodes the epidermal cell junction protein cadherin 1, was overexpressed in the muscles of IBM, which was validated by another RNA sequencing dataset from previous publications. Western blotting confirmed the presence of mature cadherin 1 protein in the muscles of IBM. Immunohistochemical staining confirmed the positivity for anti‐cadherin 1 antibody in the muscles of IBM, whereas there was no muscle fiber positive for anti‐cadherin 1 antibody in immune‐mediated necrotizing myopathy, antisynthetase syndrome, and controls. The fibers stained with anti‐cadherin 1 antibody did not have rimmed vacuoles or abnormal protein accumulation. Experimental skeletal muscle regeneration and differentiation systems showed that CDH1 is expressed during skeletal muscle regeneration and differentiation.InterpretationCDH1 was detected as a differentially expressed gene, and immunohistochemistry showed that cadherin 1 exists in the muscles of IBM, whereas it was rarely seen in those of other idiopathic inflammatory myopathies. Cadherin 1 upregulation in muscle could provide a valuable clue to the pathological mechanisms of IBM. ANN NEUROL 2022;91:317–328
Ferrocene‐Containing Nucleic Acid‐Based Energy‐Storage Nanoagent for Continuously Photo‐Induced Oxidative Stress Amplification
Authors: Ji Cailing , Li Hao , Zhang Lei , Wang Ping , Lv Yawei , Sun Zhijun , Tan Jie , Yuan Quan , Tan Weihong 
Published: January 2022

AbstractRegulation of cellular oxidative stress plays a critical role in revealing the molecular mechanisms of cellular activities and thus is a potential strategy for tumor treatment. Optical methods have been employed for intelligent regulation of oxidative stress in tumor regions. However, long‐time continuous irradiation inevitably causes damage to normal tissues. Herein, a ferrocene‐containing nucleic acid‐based energy‐storage nanoagent was designed to achieve the continuous photo‐regulation of cellular oxidative stress in the dark. Specifically, the photoenergy stored in the agent could convert effectively and accelerate Fenton‐like reaction continuously, augmenting cellular oxidative stress. This nanoagent could also silence oxidative damage repair genes to further amplify oxidative stress. This strategy not only provides oxidative stress regulation for studying the molecular mechanisms of biological activities, but also offers a promising step toward tumor microenvironment modulation.
The tension between ethics and rigor when using Amazon MTurk for eating disorder research: Response to commentaries on Burnette et al. (2021)
Authors: Burnette C. Blair , Luzier Jessica , Bennett Brooke L. , Weisenmuller Chantel , Kerr Patrick , Keener Jillian 
Published: January 2022

AbstractWe respond to commentaries on our 2021 paper “Concerns and recommendations for using Amazon MTurk for eating disorder research.” The commentators raised many thoughtful and nuanced points regarding data validity and ethical means of online data collection. We echo concerns about the ethics of recruiting via platforms such as MTurk, and highlight tensions between recommendations for ethical data collection and ensuring data integrity. Especially, we highlight the consistent finding that MTurk workers display elevated (often remarkably so) rates of psychopathology, and argue such findings merit further scrutiny to ensure both data are valid and workers not exploited.
Cardiac autonomic neuropathy and risk of incident heart failure among adults with type 2 diabetes
Authors: Kaze Arnaud D. , Yuyun Matthew F. , Erqou Sebhat , Fonarow Gregg C. , Echouffo‐Tcheugui Justin B. 
Published: January 2022

AimsCommunity‐based data on the association between cardiac autonomic neuropathy (CAN) and incident heart failure (HF) in type 2 diabetes are limited. We evaluated the association of CAN with incident HF in adults with type 2 diabetes.Methods and resultsThis analysis included participants from the Action to Control Cardiovascular Risk in Diabetes (ACCORD) study without HF at baseline. CAN was assessed by electrocardiogram‐based measures of heart rate variability (HRV) and QT interval index (QTI). HRV was measured using standard deviation of all normal‐to‐normal intervals (SDNN) and root mean square of successive differences between normal‐to‐normal intervals (rMSSD). CAN was defined using composite measures of the lowest quartile of SDNN and highest quartiles of QTI and heart rate. Multivariable Cox regression models were used to generate adjusted hazard ratios (aHR) for HF in relation to various CAN measures. A total of 7160 participants (mean age 62.3 [standard deviation 6.4] years, 40.8% women, 61.9% white) were included. Over a median follow‐up of 4.9 years (interquartile range 4.0–5.7), 222 participants developed incident HF. After multivariable adjustment for relevant confounders, lower HRV as assessed by SDNN was associated with a higher risk of HF (aHR for the lowest vs highest quartile of SDNN: 1.70, 95% confidence interval [CI] 1.14–2.54). Participants with CAN (defined as lowest quartile of SDNN and highest quartiles of QTI and heart rate) had a 2.7‐fold greater risk of HF (aHR 2.65, 95% CI 1.57–4.48).ConclusionsIn a large cohort of adults with type 2 diabetes, CAN was independently associated with higher risk of incident HF.
The impact of preformed donor‐specific antibodies in living donor liver transplantation according to graft volume
Authors: Goto Ryoichi , Ito Makoto , Kawamura Norio , Watanabe Masaaki , Ganchiku Yoshikazu , Kamiyama Toshiya , Shimamura Tsuyoshi , Taketomi Akinobu 
Published: January 2022

AbstractIntroductionThe roles of preformed anti‐HLA donor‐specific antibodies (DSAs) in liver transplantation remain controversial. We evaluated the impact of preformed DSAs in living donor liver transplantation.MethodsAdults who underwent living donor liver transplantation (n = 175) in our institute were included in this study. Lymphocyte cytotoxicity test (LCT), flow cytometric crossmatch (FCXM), and single‐antigen bead assays were performed.ResultsAmong adult living donor liver transplantation recipients, 27 (16.5%) and 14 (8.5%) had pretransplant FCXM‐positive findings and LCT‐positive findings, respectively. FCXM‐positive patients displayed a significantly worse 5‐year graft survival rate (77.3%; vs. DSA‐negative, 91.6%). Six of 14 LCT‐positive patients exhibited graft loss shortly after transplantation (5‐year survival rate: 57.1%). All LCT‐positive patients with graft loss underwent left lobe living donor liver transplantation. Significantly lower ratio of graft volume relative to standard liver volume (32.9 ± 5.7%) and smaller graft size (365.3 ± 57.9 g) were observed in patients with graft loss (p < .03, vs. surviving grafts). Significantly higher DSA‐mean fluorescence intensity (MFI) values were present in patients with graft loss (p = .0012, vs. surviving grafts).ConclusionsPatients with preformed DSAs exhibited worse graft outcomes in living donor liver transplantation. Higher DSA‐MFI values and smaller graft size were associated with worse outcomes in LCT‐positive patients. High‐risk patients with preformed DSAs should be considered for appropriate graft selection and application of a desensitization protocol.
Development of a plan complexity mitigation algorithm based on gamma passing rate predictions for volumetric‐modulated arc therapy
Authors: Ono Tomohiro , Nakamura Mitsuhiro , Ono Yuka , Nakamura Kiyonao , Mizowaki Takashi 
Published: January 2022

AbstractBackground and purposeVolumetric‐modulated arc therapy (VMAT) is a complex rotational therapy technique in which highly conformal dose distribution can be realized by varying the speed of gantry rotation, multileaf collimator (MLC) shape, and dose rate. However, the complexity of the technique creates a discrepancy between the calculated and measured doses. Thus, to mitigate the plan complexity in VMAT, this study aimed to develop an algorithm and evaluate its usefulness by conducting a feasibility study.Materials and methodsA total of 50 patients who underwent VMAT between September 2015 and December 2020 were arbitrarily selected for this study. Specifically, patients with less than 85% gamma passing rate (GPR) at 5%/1 mm or 3%/2 mm criterion were selected randomly. Using the GPR prediction model, problematic MLC positions that contribute to a decrease in GPR were identified. Those problematic MLC positions were optimized using a limited nonlinear algorithm under mechanical limitations. Additionally, the dose prescription for the target was re‐normalized. The VMAT modulated complexity score (MCSv), averaged aperture area (AA), and monitor unit per gray (MU/Gy) were evaluated as plan complexity parameters. Calculated doses in patient geometry were evaluated for the target and its surrounding region. In addition, an ArcCHECK cylindrical diode array was used to measure the dose, and GPRs at 5%/1 mm and 3%/2 mm criteria were evaluated to analyze the difference between the mitigated and original plans. The difference was calculated using the mean ± standard deviation.ResultsThe differences between the MCSv, AA, and MU/cGy values for the mitigated and original plans were 0.8 ± 1.7 (×10–2), 42.7 ± 57.9, and ‐5.6 ± 8.5, respectively. Regarding the calculated dose, the dose volume parameters were consistent within 1% for the target and the surrounding region. The differences between the mitigated and original plans were 1.8 ± 2.9% and 1.3 ± 1.8% for GPRs at 5%/1 mm and 3%/2 mm, respectively.ConclusionsThis feasibility study resulted in the development of an algorithm with the potential to mitigate plan complexity and improve the GPR for VMAT under minor leaf position modifications.
Divergence‐free finite elements for the numerical solution of a hydroelastic vibration problem
Authors: Alonso‐Rodríguez Ana , Camaño Jessika , De Los Santos Eduardo , Rodríguez Rodolfo 
Published: January 2022

AbstractIn this paper, we analyze a divergence‐free finite element method to solve a fluid–structure interaction spectral problem in the three‐dimensional case. The unknowns of the resulting formulation are the fluid and solid displacements and the fluid pressure on the interface separating both media. The resulting mixed eigenvalue problem is approximated by using appropriate basis of the divergence‐free lowest order Raviart–Thomas elements for the fluid, piecewise linear elements for the solid and piecewise constant elements for the interface pressure. It is proved that eigenvalues and eigenfunctions are correctly approximated and some numerical results are reported in order to assess the performance of the method.
Bifunctional free radical photoinitiator based on syringaldehyde
Authors: Li Qianmin , Li Huimin , Wang Zhen , Liu Jiuhong , Chen Xuquan , Liu Dayong , Song Liang , Han Yuxi , Wang Zhongwei , Yu Qing 
Published: January 2022

AbstractSyringaldehyde, a natural compound, was chosen to design a novel acylphosphine oxide (S1). Maximum absorption at 337 nm with very high molar extinction coefficient (above 13,000 M−1 cm−1) was detected for S1. As a type I photoinitiator, S1 could initiate the photopolymerization of TMPTA and ca. 50% of the double bond conversion (DC) could be obtained upon irradiation of LED@385 nm or LED@420 nm for 300 s. As coinitiator in type II system, S1 could cooperate with DETX and exhibit higher performance (ca. 60% of DC) than typical pair of DETX and EDAB. Density functional theory (DFT) is used to study the characteristics of excited state and transition manners of S1 after light absorption. Orbital distributions of singlet and triplet electron clouds, transition energy levels, and oscillator strength are also predicted. Results suggest that after intersystem crossing (ISC) and internal conversion (IC), free radicals formed through α‐cleavage (Norrish Type I reaction) or breakage of O‐H bond in S1. All results indicate that S1 could act as a bifunctional photoinitiator. Based on the above results, the roles of S1 during photoinitiation are also proposed.
Detailed investigation of the composition and transformations of phenolic compounds in fresh and fermented Vaccinium floribundum berry extracts by high‐resolution mass spectrometry and bioinformatics
Authors: Cerrato Andrea , Piovesana Susy , Aita Sara Elsa , Cavaliere Chiara , Felletti Simona , Laganà Aldo , Montone Carmela Maria , Vargas‐de‐la‐Cruz Celia , Capriotti Anna Laura 
Published: January 2022

AbstractIntroductionBlueberries are known for their very high content of biologically active phenolic compounds; nonetheless, differently from the North American and European species of blueberries, Neotropical blueberries have not been extensively studied yet.ObjectivesIn the present paper, the phenolic composition of Vaccinium floribundum Kunth, which is endemic to the Andean regions and grows 1,600 to 4,500 meters above sea level, was investigated by ultra‐high‐performance liquid chromatography coupled to high‐resolution mass spectrometry (UHPLC‐HRMS). Native and fermented berries were compared in terms of phenolic composition as well as antioxidant activity, total phenolic content, and total anthocyanin content.Materials and MethodsV. floribundum native and fermented berries were extracted and analyzed by UHPLC‐HRMS. The acquired datasets were processed by Compound Discoverer 3.1 using a dedicated data analysis workflow that was specifically set up for phenolic compound identification.ResultsIn total, 309 compounds were tentatively identified, including anthocyanins, flavonoids, phenolic acids, and proanthocyanidins. The molecular transformations of phenolic compounds during fermentation were comprehensively investigated for the first time, and by a customized data processing workflow, 13 quinones and quinone methides were tentatively identified in the fermented samples. Compared to other species of the genus Vaccinium, a peculiar phenolic profile is observed, with low abundance of highly methylated compounds.ConclusionAndean berries are a rich source of a wide variety of phenolic compounds. Untargeted MS analyses coupled to a dedicated data processing workflow allowed expanding the current knowledge on these berries, improving our understanding of the fate of phenolic compounds after fermentation.
Procalcitonin is associated with postnatal respiratory condition severity in preterm neonate
Authors: Iwai Masanori , Yoshimatsu Hidetaka , Naramura Tetsuo , Imamura Hiroko , Nakamura Tomomi , Sakamoto Rieko , Inoue Takeshi , Tanaka Kenichi , Matsumoto Shirou , Nakamura Kimitoshi , Mitsubuchi Hiroshi 
Published: January 2022

AbstractIntroductionPostnatal respiratory failure is common in preterm neonates and is difficult to distinguish from early‐onset neonatal bacterial infection by clinical symptoms. Similar to C‐reactive protein (CRP), procalcitonin (PCT) is used as a marker of bacterial infection. Recently, it has been reported that the serum PCT levels increase because of respiratory failure immediately after birth. However, there is insufficient information concerning the relationship between biological inflammation markers, such as PCT and CRP, and postnatal respiratory condition severity.MethodsPreterm neonates were classified according to the received respiratory management as follows: nonrespiratory support (NRS), respiratory support (RS), surfactant administration therapy (STA), and STA with nitric oxide inhalation therapy (NO). The median serum PCT and CRP levels at 12–36 h postnatally were compared among the four groups. Additionally, the median serum PCT and CRP levels in the STA group were compared by STA timing and STA number.ResultsThe PCT levels for the NRS, RS, STA, and NO groups were 1.04, 6.46, 12.93, and 86.79 μg/L, respectively; the CRP levels were 0.40, 0.80, 1.10, and 16.40 mg/L, respectively. The PCT levels were significantly lower among neonates receiving STA in the birth subgroup (4.82 μg/L) than among those receiving STA in the admission subgroup (14.71 μg/L). The PCT levels were significantly higher among the STA multiple‐dose subgroup (24.87 μg/L) than among the STA single‐dose subgroup (12.47 μg/L). No significant differences in the CRP levels were observed.ConclusionThe serum PCT levels in preterm neonates were associated with postnatal respiratory condition severity.
Oxygen Vacancy and Core–Shell Heterojunction Engineering of Anemone‐Like CoP@CoOOH Bifunctional Electrocatalyst for Efficient Overall Water Splitting
Authors: Zhang Bing , Shan Jiongwei , Wang Weilong , Tsiakaras Panagiotis , Li Yunyong 
Published: January 2022

AbstractConstructing cost‐efficient and robust bifunctional electrocatalysts for both neutral and alkaline water splitting is highly desired, but still affords a great challenge, due to sluggish hydrogen/oxygen evolution reaction (HER/OER) kinetics. Herein, an in situ integration engineering strategy of oxygen‐vacancy and core–shell heterojunction to fabricate an anemone‐like CoP@CoOOH core–shell heterojunction with rich oxygen‐vacancies supported on carbon paper (CoP@CoOOH/CP), is described. Benefiting from the synergy of CoP core and oxygen‐vacancy‐rich CoOOH shell, the as‐obtained CoP@CoOOH/CP catalyst displays low overpotentials at 10 mA cm‐2 for HER (89.6 mV/81.7 mV) and OER (318 mV/200 mV) in neutral and alkaline media, respectively. Notably, a two‐electrode electrolyzer, using CoP@CoOOH/CP as bifunctional catalyst to achieve 10 mA cm‐2, only needs low‐cell voltages in neutral (1.65 V) and alkaline (1.52 V) electrolyte. Besides, systematically experimental and theoretical results reveal that the core–shell heterojunction efficiently accelerates the catalytic kinetics and strengthens the structural stability, while rich oxygen‐vacancies efficiently decrease the kinetic barrier and activation energy, and reduce the energy barrier of the rate‐determining‐step for OER intermediates, thus intrinsically boosting OER performance. This work clearly demonstrates that oxygen‐vacancy and core–shell heterojunction engineering provide an effective strategy to design highly‐efficient non‐precious, bi‐functional electrocatalysts for pH‐universal water splitting.