bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dry biomass of Aspergillus niger in commercial diets for juveniles of Nile tilapia (Oreochromis niloticus) in the intermediate growth phase (15 to 70g)
Authors: Picoli Fernanda , Michielin Thayse , Sgnaulin Tayna , Costa Ana Paula de Oliveira , Nora Luisa , Marcelino Alexandre Henrique , Zampar Aline , Lopes Diogo Luiz de Alcantara , Emerenciano Mauricio Gustavo Coelho 
Published: February 2022

The objective of the present study was to evaluate different inclusion levels of drymass of the yeast Aspergillus niger (0; 0.125; 0.250; 0.500; 1.00; 2.00; 4.00 and 8.00%) in commercial diets for juveniles of Nile tilapia (16.11 ± 1.74g) in a water recirculation system. The productive performance, organosomatic indexes and carcass composition of these animals were evaluated after 58 days of experiment. A total of 192 juveniles were distributed among 32 tanks made up of eight treatments with four repetitions. A significant difference was observed for the hepatosomatic index at 2.00% of inclusion, which was similar to the other treatments except for the 0.125% inclusion level. The inclusion of A. niger had no negative impacts on performance when compared with the control diet. Since it is a by-product, the use of A. niger can be an alternative in the formulation of diets for tilapia in the initial stages (15-70g).
Adversidades identificadas pelos enfermeiros no esgotamento de acessos para terapias dialíticas
Authors: Matias Daniela de Oliveira , Lima Rafael Abrantes de , Mendes Patrícia Aparecida Tavares , Laubert Bianca Cristina Marques Gindre , Guimarães Tamires Zêba , Borges Letícia Lima , Aguiar Beatriz Gerbassi Costa 
Published: February 2022

Objetivo que norteou a pesquisa foi conhecer as dificuldades identificadas pelos enfermeiros no esgotamento de acessos para terapias dialíticas. Método: trata-se de um estudo descritivo, com abordagem qualitativa, do qual participaram 12 enfermeiros assistenciais sob escala de plantões diurno, noturno e diaristas e aprovado na Plataforma Brasil sob o nº de parecer 4.406.281. A coleta de dados foi orientada pela técnica da entrevista semiestruturada. A análise dos dados foi realizada a partir de categoria emergente proveniente do conteúdo revelado, utilizando a técnica da análise de conteúdo de Bardin. Resultados: dados da pesquisa revelaram que os enfermeiros nefrologistas identificam as dificuldades no esgotamento de acessos para terapias dialíticas bem como estresses aos pacientes renais crônicos. Conclusão: considerando a realização do estudo focalizando os enfermeiros que trabalham no setor de nefrologia julga-se importante o desenvolvimento de novos estudos para a divulgação de conhecimentos para as boas práticas do cuidado aos acessos para terapias renais substitutivas.
Applications of Mixed Reality Technology in Orthopedics Surgery: A Pilot Study
Authors: Lu Lin , Wang Honglin , Liu Pengran , Liu Rong , Zhang Jiayao , Xie Yi , Liu Songxiang , Huo Tongtong , Xie Mao , Wu Xinghuo , Ye Zhewei 
Published: February 2022

Objective: The aim of this study is to explore the potential of mixed reality (MR) technology in the visualization of orthopedic surgery.Methods: The visualization system with MR technology is widely used in orthopedic surgery. The system is composed of a 3D imaging workstation, a cloud platform, and an MR space station. An intelligent segmentation algorithm is adopted on the 3D imaging workstation to create a 3D anatomical model with zooming and rotation effects. This model is then exploited for efficient 3D reconstruction of data for computerized tomography (CT) and magnetic resonance imaging (MRI). Additionally, the model can be uploaded to the cloud platform for physical parameter tuning, model positioning, rendering and high-dimensional display. Using Microsoft’s HoloLens glasses in combination with the MR system, we project and view 3D holograms in real time under different clinical scenarios. After each procedure, nine surgeons completed a Likert-scale questionnaire on communication and understanding, spatial awareness and effectiveness of MR technology use. In addition to that, the National Aeronautics and Space Administration Task Load Index (NASA-TLX) is also used to evaluate the workload of MR hologram support.Results: 1) MR holograms can clearly show the 3D structures of bone fractures, which improves the understanding of different fracture types and the design of treatment plans; 2) Holograms with three-dimensional lifelike dynamic features provide an intuitive communication tool among doctors and also between doctors and patients; 3) During surgeries, a full lesion hologram can be obtained and blended in real time with a patient’s virtual 3D digital model in order to give surgeons superior visual guidance through novel high-dimensional “perspectives” of the surgical area; 4) Hologram-based magnetic navigation improves the accuracy and safety of the screw placement in orthopaedics surgeries; 5) The combination of mixed reality cloud platform and telemedicine system based on 5G provides a new technology platform for telesurgery collaboration. Results of qualitative study encourage the usage of MR technology for orthopaedics surgery. Analysis of the Likert-scale questionnaire shows that MR adds significant value to understanding and communication, spatial awareness, learning and effectiveness. Based on the NASA TLX-scale questionnaire results, mixed reality scored significantly lower under the “mental,” “temporal,” “performance,” and “frustration” categories compared to usual 2D.Conclusion: The integration of MR technology in orthopaedic surgery reduces the dependence on surgeons’ experience and provides personalized 3D visualization models for accurate diagnosis and treatment of orthopaedic abnormalities. This integration is clearly one of the prominent future development directions in medical surgery.
1,3,4-Oxadiazole Contained Sesquiterpene Derivatives: Synthesis and Microbiocidal Activity for Plant Disease
Authors: Dai Ali , Zheng Zhiguo , Yu Lijiao , Huang Yuanqin , Wu Jian 
Published: February 2022

A series of 1,3,4-oxadiazole contained sesquiterpene derivatives were synthesized, and the activity of the target compounds against Xanthomonas oryzae pv. oryzae (Xoo), Xanthomonas axonopodis pv. citri (Xac), and tobacco mosaic virus (TMV) were evaluated. The biological activity results showed that the EC50 values of compounds H4, H8, H11, H12, H14, H16, and H19 for Xac inhibitory activity were 33.3, 42.7, 56.1, 74.5, 37.8, 43.8, and 38.4 μg/ml, respectively. Compounds H4, H8, H15, H19, H22, and H23 had inhibitory effects on Xoo, with EC50 values of 51.0, 43.3, 43.4, 50.5, 74.6, and 51.4 μg/ml, respectively. In particular, the curative and protective activities of compound H8 against Xoo in vivo were 51.9 and 49.3%, respectively. In addition, the EC50 values of the inactivation activity of compounds H4, H5, H9, H10, and H16 against TMV were 69.6, 58.9, 69.4, 43.9, and 60.5 μg/ml, respectively. The results of molecular docking indicated that compound H10 exhibited a strong affinity for TMV-coat protein, with a binding energy of −8.88 kcal/mol. It may inhibit the self-assembly and replication of TMV particles and have an anti-TMV effect, which supports its potential usefulness as an antiviral agent.
High Albumin Level Is Associated With Regression of Glucose Metabolism Disorders Upon Resolution of Acute Liver Inflammation in Hepatitis B-Related Cirrhosis
Authors: Tian Caiyun , Zhu Yanping , Liu Yujuan , Hu Han , Cheng Qijiao , Yang Fangwan , Pei Lingqi , Zhou Yihong , Li Ying , Lin Shide 
Published: February 2022

Background and AimTo investigate the short-term dynamic changes and the factors associated with regression of glucose metabolism disorders in patients with hepatitis flare of chronic hepatitis B virus (HBV) infection.MethodsIn this study, 118 patients with severe hepatitis flare of chronic HBV infection were prospectively studied. Oral glucose tolerance test was performed on admission and during follow-up to evaluate dynamic changes in glucose metabolism disorders. The factors associated with regression of glucose metabolism disorders were identified using univariate and multivariate logistic regression analyses.ResultsThe prevalence of diabetes was significantly higher in 70 (47.1%) patients with liver cirrhosis than that in 48 (16.8%) patients without liver cirrhosis. The prevalence of impaired glucose tolerance in patients with liver cirrhosis (35.7%) was significantly lower than that in patients without liver cirrhosis (47.8%). After a follow-up of 20.0 ± 18.7 days, 28 of 31 (90.3%) patients without liver cirrhosis experienced regression of glucose metabolism disorders. Additionally, 30 (54.5%) patients with liver cirrhosis experienced regression of glucose metabolism disorders after 42.0 ± 36.2 days. In patients with liver cirrhosis, those with regression of glucose metabolism disorders had significantly higher levels of homeostasis model assessment-β-cell function, albumin (ALB), and a significantly lower level of fibrosis-4 score. ALB was identified as an independent factor associated with the regression of glucose metabolism disorders in patients with liver cirrhosis.ConclusionSevere acute liver inflammation aggravates glucose metabolism disorders in patients with hepatitis B-related liver cirrhosis and high ALB level is associated with regression of glucose metabolism disorders upon resolution of acute liver inflammation.
Simultaneous Quantitative Susceptibility Mapping of Articular Cartilage and Cortical Bone of Human Knee Joint Using Ultrashort Echo Time Sequences
Authors: Zhang Ming , Li Zhihui , Wang Hanqi , Chen Tongtong , Lu Yong , Yan Fuhua , Zhang Yuyao , Wei Hongjiang 
Published: February 2022

BackgroundIt is of great clinical importance to assess the microstructure of the articular cartilage and cortical bone of the human knee joint. While quantitative susceptibility mapping (QSM) is a promising tool for investigating the knee joint, however, previous QSM studies using conventional gradient recalled echo sequences or ultrashort echo time (UTE) sequences only focused on mapping the magnetic susceptibility of the articular cartilage or cortical bone, respectively. Simultaneously mapping the underlying susceptibilities of the articular cartilage and cortical bone of human in vivo has not been explored and reported.MethodThree-dimensional multi-echo radial UTE sequences with the shortest TE of 0.07 msec and computed tomography (CT) were performed on the bilateral knee joints of five healthy volunteers for this prospective study. UTE-QSM was reconstructed from the local field map after water-fat separation and background field removal. Spearman’s correlation analysis was used to explore the relationship between the magnetic susceptibility and CT values in 158 representative regions of interest of cortical bone.ResultThe susceptibility properties of the articular cartilage and cortical bone were successfully quantified by UTE-QSM. The laminar structure of articular cartilage was characterized by the difference of susceptibility value in each layer. Susceptibility was mostly diamagnetic in cortical bone. A significant negative correlation (r=−0.43, p&lt;0.001) between the susceptibility value and CT value in cortical bone was observed.ConclusionUTE-QSM enables simultaneous susceptibility mapping of the articular cartilage and cortical bone of human in vivo. Good association between susceptibility and CT values in cortical bone suggests the potential of UTE-QSM for bone mapping for further clinical application.
Tetrabutylammonium (TBA)-Doped Methylammonium Lead Iodide: High Quality and Stable Perovskite Thin Films
Authors: Bouich Amal , Marí-Guaita Júlia , Sahraoui Bouchta , Palacios Pablo , Marí Bernabé 
Published: February 2022

This work reported the successive incorporation of tetrabutylammonium (TBA) into Methylammonium lead Iodide (MAPbI3) perovskite. The thin films were characterized by X-Ray diffraction (XRD), Scanning electron microscopy (SEM), Transmittance electron microscopy (TEM), Atomic force microscopy (AFM), and UV-Visible spectroscopy. It was shown that introducing TBA increases the crystallinity, grain size, surface morphology without pin-hole, and roughness of the MAPbI3 thin films. Moreover, the MA(1-X)TBAX PbI3 thin film shows better stability in a relative humidity of ∼60% after 15 days than the pure MAPbI3 thin film. The obtained results are hoped to be helpful for stability and improvement of the performance of the MAPbI3 thin films by doping TBA cations under ambient conditions.
Electrospun Ternary Composite Metal Oxide Fibers as an Anode for Lithium-Ion Batteries
Authors: Ling JinKiong , Karuppiah Chelladurai , Das Santanu , Misnon Izan Izwan , Ab. Rahim Mohd Hasbi , Yang Chun-Chen , Jose Rajan 
Published: February 2022

Nickel–cobalt–manganese oxides (NCMs) are widely investigated as cathode materials for lithium-ion batteries (LIBs) given their beneficial synergistic effects of high storability, electrical conductivity, and stability. However, their use as an anode for LIBs has not been adequately addressed. NCM nanofibers prepared using the multi-needle electrospinning technique are examined as the anode in LIBs. The NCM nanofibers demonstrated an initial discharge capacity of ∼1,075 mAh g−1 with an initial capacity loss of ∼42%. Through controlling the conductive additive content, the initial discharge capacity can be further improved to ∼1810 mAh g−1, mostly attributing to the improved interfiber connectivity supported by the significant lowering of impedance when the amount of conductive additive is increased. This study also reveals that the conventional ratio of 80:10:10 wt% (active materials:additives:binder) is not optimal for all samples, especially for the high active surface area electrospun nanofibers.
The Correlated Risk Factors for Severe Liver Damage Among HIV-Positive Inpatients With Abnormal Liver Tests
Authors: Liu Sheng , Zhou Ying , Wang Yu , Li Cheng Bo , Wang Wen , Lu Xu , Liu Pei , Hu Qing Hai , Wen Ying 
Published: February 2022

Background:This study investigated the factors correlated with severe liver damage among HIV-infected inpatients.Methods:We retrospectively collected the first hospitalized HIV-infected patients in the Department of Infectious Disease of the First Affiliated Hospital of China Medical University from January 1, 2010, to December 31, 2019. We used multivariate logistic regression to identify the factors associated with severe liver damage.Results:A total of 493 patients with abnormal liver tests were recruited. Among 63 cases (12.8%) with severe liver injury, drug-induced liver injury (DILI) identified by the updated Roussel Uclaf Causality Assessment Method (RUCAM) score as the direct cause was found in 43 cases. Anti-tuberculosis drug (ATD) exposure [adjusted odds ratio (aOR) = 1.835, 95% confidence interval (CI): 1.031–3.268], cotrimoxazole exposure (aOR = 2.775, 95% CI: 1.511–5.096), comorbidity of viral hepatitis (aOR = 2.340, 95% CI: 1.161–4.716), alcohol consumption history (aOR = 2.392, 95% CI: 1.199–4.769), and thrombocytopenia (aOR = 2.583, 95% CI:1.127–5.917) were associated with severe liver injury (all P &lt; 0.05).Conclusions:DILI was the predominant cause of severe liver damage, followed by hepatitis virus co-infection. For patients with alcohol consumption and thrombocytopenia, frequent monitoring of liver function tests should be considered.
Arterial Hypotension Following Norepinephrine Decrease in Septic Shock Patients Is Not Related to Preload Dependence: A Prospective, Observational Cohort Study
Authors: Andrei Stefan , Nguyen Maxime , Abou-Arab Osama , Bouhemad Belaid , Guinot Pierre-Grégoire 
Published: February 2022

BackgroundThe optimal management of hypotensive patients during norepinephrine weaning is unclear. The primary study aim was to assess the ability of preload dependence to predict hypotension following norepinephrine weaning. The secondary aims were to describe the effect of norepinephrine weaning on preload dependence, and the cardiovascular effects of fluid expansion in hypotensive patients following norepinephrine weaning.Materials and MethodsThis was a prospective observational monocentric study. We included PiCCO®-monitored patients with norepinephrine-treated septic shock, for whom the physician decided to decrease the norepinephrine dosage during the de-escalation phase. Three consecutive steps were evaluated with hemodynamic measurements: baseline, after norepinephrine decrease, and after 500 mL fluid expansion.ResultsForty-five patients were included. Preload dependence assessed by stroke volume changes following passive leg raising was not predictive of pressure response to norepinephrine weaning [AUC of 0.42 (95%CI: 0.25–0.59, p = 0.395)]. After fluid expansion, there was no difference in the prior preload dependence between pressure-responders and non-pressure-responders (14 vs. 13%, p = 1). The pressure response to norepinephrine decrease was not associated with pressure response after fluid expansion (40 vs. 23%, p = 0.211).ConclusionHypotension following norepinephrine decrease was not predicted by preload dependence, and there was no association between arterial hypotension after norepinephrine decrease and fluid response.
Polymicrobial Anaerobic Meningitis Detected by Next-Generation Sequencing: Case Report and Review of the Literature
Authors: Li Xiaoqiang , Du Hui , Song Zhibin , Wang Hui , Long Xiong 
Published: February 2022

BackgroundAnaerobic meningitis is a severe central nervous system infection associated with significant neurological sequelae and high mortality. However, the precise detection of causative pathogen(s) remains difficult because anaerobic bacteria are difficult to culture. Next-generation sequencing is a technology that was developed recently and has been applied in many fields. To the best of our knowledge, the use of next-generation sequencing for cerebrospinal fluid analysis in the diagnosis of anaerobic meningitis has been rarely reported.Case presentationHere, we report a case of polymicrobial anaerobic meningitis diagnosed using next-generation sequencing of cerebrospinal fluid in a 16-year-old girl. Five species of anaerobic bacteria (Porphyromonas gingivalis, Prevotella enoeca, Campylobacter rectus, Fusobacterium uncleatum, and Actinomyces israelii) were detected by next-generation sequencing and treated with antibacterial agents (ceftriaxone, vancomycin, and metronidazole). The patient responded well to antibacterial treatment. Further inspection revealed bone destruction at the base of the skull, which further confirmed that these bacteria had originated from the oral cavity. One month later, the patient's condition improved significantly. At the same time, we performed a literature review on anaerobic meningitis using studies published in the last 20 years.ConclusionsThis case emphasizes the importance of applying metagenomic next-generation sequencing to clinch the clinical diagnosis for patients with central nervous system infection. Metagenomic next-generation sequencing has been reported to be an important diagnostic modality for identifying uncommon pathogens.
Perspectives on Bulk-Tissue RNA Sequencing and Single-Cell RNA Sequencing for Cardiac Transcriptomics
Authors: Hegenbarth Jana-Charlotte , Lezzoche Giuliana , De Windt Leon J. , Stoll Monika 
Published: February 2022

The heart has been the center of numerous transcriptomic studies in the past decade. Even though our knowledge of the key organ in our cardiovascular system has significantly increased over the last years, it is still not fully understood yet. In recent years, extensive efforts were made to understand the genetic and transcriptomic contribution to cardiac function and failure in more detail. The advent of Next Generation Sequencing (NGS) technologies has brought many discoveries but it is unable to comprehend the finely orchestrated interactions between and within the various cell types of the heart. With the emergence of single-cell sequencing more than 10 years ago, researchers gained a valuable new tool to enable the exploration of new subpopulations of cells, cell-cell interactions, and integration of multi-omic approaches at a single-cell resolution. Despite this innovation, it is essential to make an informed choice regarding the appropriate technique for transcriptomic studies, especially when working with myocardial tissue. Here, we provide a primer for researchers interested in transcriptomics using NGS technologies.
Exosomal miR-29b of Gut Origin in Patients With Ulcerative Colitis Suppresses Heart Brain-Derived Neurotrophic Factor
Authors: Lian Haifeng , Zhong Xiaoying S. , Xiao Ying , Sun Zhe , Shen Yuanyuan , Zhao Kaile , Ma Xingbin , Li Yanmin , Niu Qiong , Liu Max , Powell Don W. , Liu Chengxia , Li Qingjie 
Published: February 2022

Background and Aims: While the interplay between heart and gut in inflammatory bowel disease (IBD) has previously been noted, how the inflamed gut impairs heart function remain elusive. We hypothesized that exosomal miRNAs of gut origin induce cardiac remodeling in IBD. Our aim was to identify plasma exosomal miRNAs that not only are of diagnostic value but also contribute to cardiac remodeling in patients with ulcerative colitis (UC).Methods: Plasma exosomes were isolated from UC patients and healthy control subjects and exosomal miRNAs were profiled by next-generation sequencing. Exosomal miR-29b levels in CCD841 CoN colon epithelial cells were detected by RT-qPCR. Exosomes packaged with miR-29b were incubated with H9c2 cells or administered to live mice.Results: The plasma exosomal miRNA profiles of the UC patients were significantly different from that of the controls and 20 miRNAs including miR-29b were differentially expressed. In CCD841 CoN cells, TNFα, IL-1β, and H2O2 significantly elevated miR-29b in both the cells and their secreted exosomes (p &lt; 0.01), suggesting that intestinal epithelium secrets exosomes rich in miR-29b in IBD. In H9c2 myoblast cells, miR-29b modulated multiple genes including brain-derived neurotrophic factor (BDNF). Epithelial cell-derived exosomes packaged with miR-29b also attenuated BDNF and increased cleaved caspase 3, suggestive of apoptosis. Furthermore, tail vein injection of engineered exosomes with high levels of miR-29b suppressed BDNF and augmented cleaved caspase 3 in the heart of adult mouse (p &lt; 0.01).Conclusion: Plasma exosomal miRNA profile could be a novel diagnostic approach for IBD. Excessive plasma exosomal miR-29b suppresses critical proteins like BDNF in IBD, leading to cardiac impairment.