bluuebunny/crossref_metadata_2025_split huggingface dataset:
From car use reduction to ride‐sharing: The relevance of moral and environmental identity
Authors: Culiberg Barbara , Cho Hichang , Kos Koklic Mateja , Zabkar Vesna 
Published: June 2022

AbstractCar use contributes significantly to environmental pollution, leading people to rethink their transport habits. Previous studies on car use reduction relied on established theories related to values and self‐regulation to examine this decision. In this paper, we offer an alternative approach and examine the intention to reduce car use through the prism of identity theory, and propose environmental and moral identities, together with environmental concern, as the main antecedents to the importance of car use reduction and intention to reduce car use. Further, we consider ride‐sharing intention as an outcome of the decision to reduce car use. The proposed conceptual model is tested on a sample of consumers from the United Kingdom using an online consumer panel. The results offer partial support for the influence of moral identity, environmental identity, and environmental concern on the importance to reduce car use and intention. The links among the importance of and intention to reduce car use, along with ride‐sharing intentions, are supported. Finally, managerial implications are discussed, and future research directions are suggested.
Nickel‐Catalysed Cross‐Electrophile Coupling of Benzyl Bromides and Sulfonium Salts towards the Synthesis of Dihydrostilbenes
Authors: del Río‐Rodríguez Roberto , Blanco Laura , Collado Alba , Fernández‐Salas Jose A. , Alemán José 
Published: June 2022

AbstractA nickel‐catalysed reductive cross‐coupling reaction between benzyl sulfonium salts and benzyl bromides is reported. Simple, stable and readily available sulfonium salts have shown their ability as leaving groups in cross‐electrophile coupling, allowing the formation of challenging sp3–sp3 carbon‐carbon bonds, towards the synthesis of interesting dihydrostilbene derivatives. In addition, benzyl tosyl derivatives have been demonstrated to be suitable substrates for reductive cross‐coupling by in‐situ formation of the corresponding sulfonium salt.
A novel quality‐related process monitoring method for multi‐unit industrial processes under incomplete data conditions
Authors: Zhang Chuanfang , Dong Jie , Peng Kaixiang , Zhang Xueyi 
Published: June 2022

AbstractModern industrial processes usually consist of multiple local units. With the wide application of distributed control systems, a large amount of industrial data is collected. During data acquisition and transmission, outliers and missing data commonly exist in different local units. Under such incomplete data conditions, the detection performance of traditional quality‐related process monitoring methods may be seriously degraded. This study develops a novel quality‐related process monitoring method for multi‐unit industrial processes. Considering the existence of outliers and missing data, incomplete data preprocessing is first performed on raw process data and quality data. Then, process variables are divided into several blocks according to the process knowledge. Afterwards, the quality‐related features extracted by variational information bottleneck (VIB) are used to train deep support vector data description (DSVDD). Finally, a quality‐related process monitoring strategy is designed at both block and global levels. Experimental results on the revised Tennessee Eastman process demonstrate that the proposed method has better monitoring performance under incomplete data conditions than other state‐of‐the‐art methods.
Extremum‐seeking control for unknown static maps with mixed actuator nonlinearity
Authors: Guay Martin 
Published: June 2022

AbstractThis study proposes an extremum‐seeking controller design for unknown static maps subject to a combination of saturated and discrete actuators with unknown characteristics but with measurable final control elements. The proposed design techniques incorporate a filter for each uncertain actuator that enables the design of a constrained extremum‐seeking control that operates at a slow time‐scale. The stability analysis demonstrates that the closed‐loop system has a practically asymptotically stable equilibrium at the unknown constrained optimum of the optimization problem. A simulation is presented that demonstrates the effectiveness of the proposed technique.
Stage‐based approach to predict left ventricular reverse remodeling after mitral repair
Authors: Hibino Makoto , Dhingra Nitish K. , Chan Vincent , Mazer C. David , Teoh Hwee , Quan Adrian , Verma Raj , Leong‐Poi Howard , Bisleri Gianluigi , Connelly Kim A. , Verma Subodh 
Published: June 2022

AbstractBackgroundAlthough predictors of reverse left ventricular (LV) remodeling postmitral valve repair are critical for guiding perioperative decision‐making, there remains a paucity of randomized, prospective data to support the criteria that potential predictor variables must meet.Methods and ResultsThe CAMRA CardioLink‐2 randomized trial allocated 104 patients to either leaflet resection or preservation strategies for mitral repair. The correlation of indexed left ventricular end‐systolic volume (LVESVI), indexed left ventricular end‐diastolic volume (LVEDVI), and left ventricular ejection fraction (LVEF) were tested with univariate analysis and subsequently with multivariate analysis to determine independent predictors of reverse remodeling at discharge and at 12 months postoperatively. At discharge, both LVESVI and LVEDVI were independently associated with their preoperative values (p < .001 for both) and LVEF by preoperative LVESVI (p < .001). Mitral ring size was favorably associated with the change in LVESVI (p < .05) and LVEF (p < .01) from predischarge to 12 months, while the mean mitral valve gradient after repair was adversely associated with the change in LVESVI (p < .05) and LVEDVI (p < .05). No significant associations were found between reverse remodeling and coaptation height nor mitral repair technique.ConclusionsBeyond confirming the lack of impact of mitral repair technique on reverse remodeling, this investigation suggests that recommending surgery before significant LV dilatation or dysfunction, as well as higher postoperative mitral valve hemodynamic performance, may enhance remodeling capacity following mitral repair.
Lipid metabolism–related lncRNA SLC25A21‐AS1 promotes the progression of oesophageal squamous cell carcinoma by regulating the NPM1/c‐Myc axis and SLC25A21 expression
Authors: Liu Yu , Li Chunxiang , Fang Lingling , Wang Liyu , Liu Hengchang , Tian He , zheng Yujia , Fan Tao , He Jie 
Published: June 2022

AbstractBackgroundObesity alters metabolic microenvironment and is thus associated with several tumours. The aim of the present study was to investigate the role, molecular mechanism of action, and potential clinical value of lipid metabolism–related long non‐coding RNA (lncRNA) SLC25A21‐AS1 in oesophageal squamous cell carcinoma (ESCC).MethodsA high‐fat diets (HFDs)‐induced obesity nude mouse model was established, and targeted metabolomics analysis was used to identify critical medium‐long chain fatty acids influencing the growth of ESCC cells. Transcriptomic analysis of public dataset GSE53625 confirmed that lncRNA SLC25A21‐AS1 was a lipid metabolism–related lncRNA. The biological function of lncRNA SLC25A21‐AS1 in ESCC was investigated both in vivo and in vitro. Chromatin immunoprecipitation(ChIP)assay, RNA‐pull down, mass spectrometry, co‐IP, and RNA IP(RIP) were performed to explore the molecular mechanism. Finally, an ESCC cDNA microarray was used to determine the clinical prognostic value of SLC25A21‐AS1 by RT‐qPCR.ResultsPalmitic acid (PA) is an important fatty acid component of HFD and had an inhibitory effect on ESCC cell lines. LncRNA SLC25A21‐AS1 expression was downregulated by PA and associated with the proliferation and migration of ESCC cells in vitro and in vivo. Mechanistically, SLC25A21‐AS1 interacted with nucleophosmin‐1 (NPM1) protein to promote the downstream gene transcription of the c‐Myc in the nucleus. In the cytoplasm, SLC25A21‐AS1 maintained the stability of SLC25A21 mRNA and reduced the intracellular NAD+/NADH ratio by influencing tryptophan catabolism. Finally, we demonstrated that high expression of SLC25A21‐AS1 promoted resistance to cisplatin‐induced apoptosis and was correlated with poor tumour grade and overall survival.ConclusionsHFD/PA has an inhibitory effect on ESCC cells and SLC25A21‐AS1 expression. SLC25A21‐AS1 promotes the proliferation and migration of ESCC cells by regulating the NPM1/c‐Myc axis and SLC25A21 expression. In addition, lncRNA SLC25A21‐AS1 may serve as a favourable prognostic biomarker and a potential therapeutic target for ESCC.
Integrated microbiome, metabolome, and proteome analysis identifies a novel interplay among commensal bacteria, metabolites and candidate targets in non‐small cell lung cancer
Authors: Qian Xiang , Zhang Hong‐Yan , Li Qing‐Lin , Ma Guan‐Jun , Chen Zhuo , Ji Xu‐Ming , Li Chang‐Yu , Zhang Ai‐qin 
Published: June 2022

AbstractBackgroundAccumulation of evidence suggests that the gut microbiome, its specific metabolites, and differentially expressed proteins (DEPs) are related to non‐small cell lung cancer (NSCLC) pathogenesis. We now report the influences of the gut microbiota, metabolites, and DEPs on the mediation of NSCLC's chronic inflammation and immune dysregulation.MethodsWe conducted 16S ribosomal RNA sequencing for the gut microbiome in healthy volunteers and NSCLC patients. Liquid chromatography–mass spectrometry (LC–MS) analysis was employed to explore differences between metabolites and DEPs in serum samples. Additionally, LC–MS‐based metabolomic analysis was conducted in 40 NSCLC tissues and 40 adjacent tissues. The omics data were separately analysed and integrated by using Spearman's correlation coefficient. Then, faecal microbiota transplantation (FMT) assay was used to assess the effects of the gut microbiome and specific metabolites in mice.ResultsFaecal microbiome analysis revealed gut microflora dysbiosis in NSCLC patients with Prevotella, Gemmiger, and Roseburia significantly upregulated at the genus level. Then, we identified that nervonic acid/all‐trans‐retinoic acid level was negatively related to Prevotella. Additionally, a total of core 8 DEPs were selected in the proteome analysis, which mainly participated in the production of IL‐8 and NF‐κB pathways. CRP, LBP, and CD14 were identified as potential biomarkers for NSCLC. Transplantation of faecal microbiota from patients with NSCLC or Prevotella copri‐colonized recipient in mice resulted in inflammation and immune dysregulation. In turn, nervonic acid/all‐trans‐retinoic acid treatment improved the phenotype of C57BL/6 mice bearing P. copri‐treated Lewis lung cancer (LLC).ConclusionsOverall, these results pointed out that P. copri‐nervonic acid/all‐trans‐retinoic acid axis may contribute to the pathogenesis of NSCLC.
Attachment insecurity predicts worse outcome in patients with eating disorders treated with enhanced cognitive behavior therapy: A one‐year follow‐up study
Authors: Rossi Eleonora , Cassioli Emanuele , Martelli Michela , Melani Giulia , Hazzard Vivienne M. , Crosby Ross D. , Wonderlich Stephen A. , Ricca Valdo , Castellini Giovanni 
Published: June 2022

AbstractObjectiveThis study aimed to explore the role of attachment insecurity in predicting a worse longitudinal trend of eating disorder (ED) psychopathology and body uneasiness in patients with Anorexia Nervosa (AN) or Bulimia Nervosa (BN) treated with Enhanced Cognitive Behavior Therapy, considering the longitudinal interplay between these dimensions.MethodIn total, 185 patients with AN or BN performed the baseline assessment, and 123 were re‐evaluated after 1 year of treatment. Participants completed questionnaires evaluating ED psychopathology (Eating Disorders Examination Questionnaire) and body uneasiness (body uneasiness test). For the assessment of adult attachment, the Experiences in Close Relationships‐Revised was administered at baseline. Bivariate latent change score analysis within the structural equation modeling framework was performed to investigate the evolution of ED psychopathology and body uneasiness, their longitudinal interplay, and the role of attachment style as an outcome predictor.ResultsAfter treatment, all psychopathological features showed an overall improvement. Higher baseline levels of body uneasiness predicted a worse course of ED psychopathology. The change in body uneasiness over time depended on changes over time in ED psychopathology, but not vice versa. Insecure attachment predicted a worse longitudinal trend of ED psychopathology, and, through this impairment, it indirectly maintained higher levels of body uneasiness, as confirmed by mediation analyses.DiscussionThe role of attachment insecurity as a predictor of treatment outcome suggests the need for an integration of the cognitive‐behavioral conceptualization of EDs with a developmental perspective that considers attachment‐related issues.Public significance statementConsidering the burden of EDs in terms of public health and the unsatisfactory response to standard treatments, the identification of outcome predictors is of considerable clinical interest. This study demonstrated that attachment insecurity was associated with worse longitudinal trends of ED psychopathology and body uneasiness in patients with AN and BN treated with CBT‐E, highlighting the importance of personalizing treatment programs taking into account a developmental perspective on these disorders.
Characterization of microRNA and gene expression in the cochlea of an echolocating bat (Rhinolophus affinis)
Authors: Li Qianqian , Chen Wenli , Mao Xiuguang 
Published: June 2022

AbstractMicroRNAs (miRNAs) are important post‐transcriptional regulators of gene expression and play key roles in many biological processes, such as development and response to multiple stresses. However, little is known about their roles in generating novel phenotypes and phenotypic variation during the course of animal evolution. Here, we, for the first time, characterized the miRNAs of the cochlea in an echolocating bat (Rhinolophus affinis). We sampled eight individuals from two R. affinis subspecies with significant echolocation call frequency differences. We identified 365 miRNAs and 121 of them were novel. By searching sequences of these miRNAs precursors in multiple high‐quality mammal genomes, we found one specific miRNA shared by all echolocating bats but not present in all other nonecholocating mammals. The targeted genes of this miRNA included several known hearing genes (e.g., KCNQ4 and GJB6). Together with the matched mRNA‐seq data, we identified 1766 differentially expressed genes (DEGs) between the two subspecies and 555 of them were negatively regulated by differentially expressed miRNAs (DEMs). We found that almost half of known hearing genes in the list of all DEGs were regulated negatively by DEMs, suggesting an important role of miRNAs in call frequency variation of the two subspecies. These targeted DEGs included several important hearing genes (e.g., Piezo1, Piezo2, and CDH23) that have been shown to be important in ultrasonic hearing of echolocating mammals.
Retrogressive thaw slumps in the Alaskan Low Arctic may influence tundra shrub growth more strongly than climate
Authors: Huebner Diane C. , Buchwal Agata , Bret‐Harte M. Syndonia 
Published: June 2022

AbstractThermokarst disturbance in permafrost landscapes is likely to increase across the tundra biome with climate warming, resulting in changes to topography, vegetation, and biogeochemical cycling. Tundra shrubs grow on permafrost, but shrub–thermokarst relationships are rarely studied in detail. Since the 1980s, Alaska's North Slope has experienced increased thermokarst activity, including retrogressive thaw slumps (RTSs) on hillslopes. Within decades, RTSs near Toolik Lake, Alaska, were colonized by tall (≥0.5 m) deciduous shrubs. We used dendrochronology methods on 66 shrubs (182 stem cross sections) representing dominant deciduous species: willows (Salix pulchra and S. glauca) and dwarf birch (Betula nana) at two RTS chronosequences on Alaska's North Slope comprising seven sites, to quantify thermokarst and climate effects (25 years of temperature and precipitation records) on shrub secondary growth (i.e., annual rings) in RTS‐disturbed and undisturbed moist acidic tussock (MAT) tundra. Across species, average growth ring widths were two times wider for shrubs in RTSs than in MAT, and ring widths decreased with RTS age. A 1°C June temperature increase was associated with 2% wider rings across species and sites, but shrubs showed marginal growth in warmer summers, supporting tundra‐wide shrub climate sensitivity studies. A 4.5% average ring width increase per 1 mm of previous year's September precipitation was seen in shrubs in mid‐successional RTSs, suggesting protective effects of early snowfall in RTSs versus open tundra. Retrogressive thaw slump age category explained 47% and 30% of average ring width variance of willows and dwarf birch, respectively, in linear mixed‐effects models. Climate variables explained 2% average ring width variance across species. Our results suggest that RTS exerts strong successional effects on tundra shrub growth. Climate effects appear to show weaker synoptic patterns across the study area. Retrogressive thaw slumps will likely contribute to tundra greening where RTS activity is increasing.
Graphene‐based Disposable Electrochemical Sensor for Chlorhexidine Determination
Authors: Magdy Nancy , Sobaih Ahmed E. , Hussein Lobna A. , Mahmoud Amr M. 
Published: June 2022

AbstractIn this work, simple, reliable, eco‐friendly, and quantitative electrochemical sensors were developed to detect chlorhexidine Digluconate (CHX) in a variety of dosage forms, including mouthwashes and intimate douches, as well as chlorhexidine in spiked human saliva. Without any sample pre‐treatment or extraction processes, CHX was measured in colored aqueous formulations. Based on carbon screen‐printed electrodes, two potentiometric sensors (sensors I and II), utilizing graphene nanocomposites (Gr‐NC), were designed (SPEs). An ionophore, 2‐hydroxypropyl‐β‐cyclodextrin, was doped into the Poly Vinyl Chloride (PVC) polymeric membrane to improve sensor selectivity.
An efficient ancestry informative SNPs panel for further discriminating East Asian populations
Authors: Cao Yueyan , Zhu Qiang , Huang Yuguo , Li Xi , Wei Yifan , Wang Haoyu , Zhang Ji 
Published: June 2022

AbstractIn forensic genetics, the use of ancestry informative single‐nucleotide polymorphisms (AISNPs) panels can narrow the direction of the investigation by estimating an individual's biogeographic ancestry. However, distinguishing subgroups within continental regions requires more specific panels. In this study, we screened 19 AISNPs from the 1000 Genomes Project (1KG) based on their FST values to distinguish target populations in East Asia and obtained genotypes through SNaPshot. The 19 AISNPs could divide the global population of the 1KG into five clusters and could further divide the East Asian population into four clusters: Japanese, Han Chinese, Dai Chinese, and Kinh in Ho Chi Minh City of Vietnam. In summary, the 19‐AISNP panel may serve as a useful and cost‐effective tool for forensic ancestry inference in East Asian populations at a finer scale.
Advanced tools for guiding data‐led research processes of Upper‐Atmospheric phenomena
Authors: Tanaka Yoshimasa , Umemura Norio , Abe Shuji , Shinbori Atsuki , UeNo Satoru 
Published: June 2022

AbstractThis paper presents tools that help researchers implement the processes of data‐led studies of upper‐atmospheric phenomena. These tools were developed as a part of the activities of the Inter‐university Upper atmosphere Global Observation NETwork (IUGONET) of Japan, which is a project to develop infrastructure for upper‐atmospheric research data. This paper focuses on the data service named IUGONET Type‐A, which was launched in October 2016 and has since evolved. In addition to being a conventional metadata catalogue, it has many other useful functions: an easy cross‐searching system, a quick‐look data‐plotting procedure, an interactive data visualization system named UDAS web, and strong linkage with analysis software. Users can pick up relevant data from a huge number of data sets using either lists categorized by instruments/projects, observed regions and special campaigns or a world map of observatories. Users can quickly find the time, location and nature of phenomena that occurred by comparing the quick‐look plots of various data displayed by the browser. UDAS web allows researchers to interactively create stacked plots of various data types that can facilitate the understanding of the relationships among phenomena observed in different regions. Furthermore, it presents a command list for software dedicated to data analysis that can smoothly lead users to perform detailed analyses. IUGONET Type‐A provides a one‐stop data service that can assist users in searching, examining and comprehending data for advanced analysis. It is also capable of handling old data, including analogue data and written paper documents. Thus, it will provide useful support for innovative interdisciplinary scientific research on solar–terrestrial phenomena.
Apolipoprotein F is reduced in humans with steatosis and controls plasma triglyceride‐rich lipoprotein metabolism
Authors: Deprince Audrey , Hennuyer Nathalie , Kooijman Sander , Pronk Amanda C. M. , Baugé Eric , Lienard Viktor , Verrijken An , Dirinck Eveline , Vonghia Luisa , Woitrain Eloïse , Kloosterhuis Niels J. , Marez Eléonore , Jacquemain Pauline , Wolters Justina C. , Lalloyer Fanny , Eberlé Delphine , Quemener Sandrine , Vallez Emmanuelle , Tailleux Anne , Kouach Mostafa , Goossens Jean‐Francois , Raverdy Violeta , Derudas Bruno , Kuivenhoven Jan Albert , Croyal Mikaël , van de Sluis Bart , Francque Sven , Pattou François , Rensen Patrick C. N. , Staels Bart , Haas Joel T. 
Published: June 2022

Background:
NAFLD affects nearly 25% of the global population. Cardiovascular disease (CVD) is the most common cause of death among patients with NAFLD, in line with highly prevalent dyslipidemia in this population. Increased plasma triglyceride (TG)‐rich lipoprotein (TRL) concentrations, an important risk factor for CVD, are closely linked with hepatic TG content. Therefore, it is of great interest to identify regulatory mechanisms of hepatic TRL production and remnant uptake in the setting of hepatic steatosis.

Approach and Results:
To identify liver‐regulated pathways linking intrahepatic and plasma TG metabolism, we performed transcriptomic analysis of liver biopsies from two independent cohorts of obese patients. Hepatic encoding apolipoprotein F (APOF) expression showed the fourth‐strongest negatively correlation with hepatic steatosis and the strongest negative correlation with plasma TG levels. The effects of adenoviral‐mediated human ApoF (hApoF) overexpression on plasma and hepatic TG were assessed in C57BL6/J mice. Surprisingly, hApoF overexpression increased both hepatic very low density lipoprotein (VLDL)‐TG secretion and hepatic lipoprotein remnant clearance, associated a ~25% reduction in plasma TG levels. Conversely, reducing endogenous ApoF expression reduced VLDL secretion in vivo, and reduced hepatocyte VLDL uptake by ~15% in vitro. Transcriptomic analysis of APOF‐overexpressing mouse livers revealed a gene signature related to enhanced ApoB‐lipoprotein clearance, including increased expression of Ldlr and Lrp1, among others.

Conclusion:
These data reveal a previously undescribed role for ApoF in the control of plasma and hepatic lipoprotein metabolism by favoring VLDL‐TG secretion and hepatic lipoprotein remnant particle clearance.