bluuebunny/crossref_metadata_2025_split huggingface dataset:
CTBP1 ve NF2 Gen Ekspresyonlarının Prostat Kanser Hücrelerinde KDM6A/B Aracılı Epigenetik Regülasyonunun İncelenmesi
Authors: YILDIRIM BUHARALIOĞLU Gökçe 
Published: March 2022

Amaç: Çalışmanın amacı, epigenetik regülatörlerden lizin demetilaz 6A (KDM6A) ve/veya lizin demetilaz 6B’nin (KDM6B) prostat kanseri oluşumu ve progresyonunda rol oynayan C Terminal bağlayıcı protein1 (CTBP1) ve Nörofibromatozis Tip2 (NF2) ekspresyonlarının regülasyonu üzerindeki modüle edici rolünün aydınlatılmasıdır.
Yöntemler: Prostat kanseri metastatik hücreleri LNCaP’lere KDM6 ailesi selektif inhibitörü GSK-J4 uygulanıp, total RNA izolasyonu ve cDNA sentezi yapılarak; CTBP1 ve NF2 mRNA düzeylerindeki değişiklikler reverse transcription-quantitative real time PCR (RT-qPCR) ile gösterilmiştir. Saptanan değişikliklerin transkripsiyonel inhibisyondan kaynaklanıp kaynaklanmadığını araştırmak üzere CTBP1 ve NF2 pre-spliced mRNA düzeylerindeki değişiklikler RT-qPCR ile ölçülmüştür. KDM6A ve/veya KDM6B’nin (KDM6A/B) small interfering RNA (siRNA) aracılı susturulduğu hücrelerde CTBP1 ve NF2 mRNA düzeylerindeki değişiklikler RT-qPCR ile gösterilerek bu genlerin KDM6 enzimlerinin hangisi tarafından regüle edildiği belirlenmiştir.
Bulgular: CTBP1 ve NF2 rölatif mRNA düzeyleri GSK-J4 ile %45 ve %49 azalmıştır. Saptanan azalmaların CTBP1 ve NF2 mRNA ekspresyonlarının GSK-J4 tarafından transkripsiyonel baskılanmasıyla ilişkili olup olmadığını belirlemek üzere yapılan RT-qPCR deneylerinde CTBP1 ve NF2 pre-spliced mRNA düzeyleri de %43, %29 azalmıştır. GSK-J4’ün KDM6 ailesine selektif bir inhibitör olması nedeniyle CTBP1 ve NF2 mRNA ekspresyonlarının hangi KDM6 enzimi tarafından kontrol edildiğini belirlemek amacıyla KDM6A ve/veya KDM6B susturulduğunda; KDM6A ve KDM6B dual inhibisyonunda CTBP1 ve NF2 mRNA düzeyleri %56, %39 azalmıştır.
Sonuç: Özetle; GSK-J4 uygulanmasıyla CTBP1 ve NF2 mRNA düzeylerinde saptadığımız azalmaların transkripsiyondaki değişiklikten kaynaklandığı pre-spliced mRNA verilerimizle güçlü bir şekilde desteklenmiştir. KDM6A ve KDM6B’nin her ikisinin de CTBP1 ve NF2 ekspresyonlarının prostat kanserindeki regülasyonunda kontrol edici rollerinin olduğunun gösterilmesi; terapötik olarak hedeflenebilecek, KDM6A ve KDM6B aracılığıyla CTBP1 ve NF2 ekspresyonlarını modüle eden yeni bir mekanizmanın aydınlatılmasına katkı sağlayabilir.
hCG Günü Serum Estradiol ve Progesteron Değerleri, Antagonist Protokollü IVF-ICSI Sikluslarında Tedavi Başarısını Etkiler mi?
Authors: DEĞER Uğur , ÇAVUŞ Yunus , OKUTUCU Gülcan , PEKER Nurullah 
Published: March 2022

Amaç: GnRH antagonist protokollü IVF-ICSI sikluslarında hCG günü çalışılan serum estradiol ve progesteron değerlerinin tedavi sürecini, implantasyon ve klinik gebelik oranlarını nasıl etkilediğini araştırmak amaçlandı.
Yöntemler: İkinci basamak bir merkezde, 2019-2020 yılları arasında kontrollü ovaryan stimülasyon (KOS) sonrası ICSI tedavisi uygulanan IVF-embriyo transferi (ET) siklusu yapılan hastalar retrospektif olarak tarandı. Hastalar, hCG uygulandığı güne ait serum östradiol (E2) seviyesine göre üç gruba ayrıldı: Grup A (4000 pg/ml). Ayrıca, hCG uygulanan güne ait serum P4 seviyesine göre de
Taxonomic review of Kokeshia Miyamoto, 1960 from China, with description of ten new species (Hemiptera: Heteroptera: Schizopteridae)
Authors: Luo Jiu-Yang , Xie Qiang 
Published: March 2022

The species of Kokeshia Miyamoto, 1960 from China are reviewed, K. hsiaoi Ren & Zheng, 1992 is redescribed, and ten new species from China are described. Among these new species, K. baii sp. nov., was collected from Hong Kong, and the other nine, K. acutiformis sp. nov., K. bui sp. nov., K. caii sp. nov., K. drepanoides sp. nov., K. hilli sp. nov., K. pengae sp. nov., K. redeii sp. nov., K. renae sp. nov. and K. weirauchae sp. nov., from Yunnan. Thus, the number of known species of this genus is increased from eight to eighteen. Photographs of the habitus, forewing, abdomen and genitalic structures; drawings and scanning electron micrographs of male abdomen and genitalic structures are provided. A key to all the species currently recognized in the genus Kokeshia is presented, a distribution map and checklist for all known species is provided.
Risk Factors for Undernutrition among Children in South Central Somalia
Authors: Zikusooka Monica , Tawfik Hebatullah , Robare Joseph 
Published: March 2022

Objectives: Undernutrition is a global public health challenge, especially in countries that experience extreme climate conditions and armed conflict. In Somalia, undernutrition is chronic, often graded for emergency response. The purpose of this study was to provide evidence on immediate, proximate, and distal risk factors for undernutrition in the most affected region of Somalia.
Setting: Data for the study was from cross-sectional nutritional surveys implemented by the Somalia Food Security and Nutrition Analysis Unit. Sampling for the surveys followed a multistage cluster sampling methodology where in the first stage, 30 clusters were randomly assigned to villages, and then 30 households were randomly selected from each cluster. Generalized Estimation Equations were used to determine risk factors for undernutrition. Data analysis followed survey analysis procedures.
Participants: 60,856 children aged 6-59 months from cross-sectional nutritional surveys implemented in South-Central Somalia from 2007 to 2012.
Results: When factors at the individual, household, and society level were considered simultaneously, diarrhea diseases and geographical region were the main risk factors for underweight, child gender, meal frequency, and livelihood zone were risk factors for stunting, while diarrhea and livelihood zone were the risk factors for wasting. Geographical region and livelihood system were significant factors for undernutrition.
Conclusions: Interventions to address undernutrition in Somalia should be tailored to the region and livelihood zone while prioritizing innovative climate-smart food production and addressing childhood illnesses. The study findings provide evidence to inform nutrition policy and programs that could eliminate nutrition disparities and the burden of childhood undernutrition in Somalia and other countries with similar contexts.
Magnitudes of Immunization Dropout Rate and Predictors for 12-23 Months Aged Children in Abobo District Southwest Ethiopia
Authors: Kassaw Ayalew , Mariam Abebe Gebere , Kebede Alemi , Kebede Fassikaw 
Published: March 2022

Vaccination is the epicenter of preventive care for good children's health outcomes in each nation. Nevertheless, a number of factors have been hindering the attainment of targets from providing complete vaccination in different nations. This study aims to assess predictors of immunizations in 12-23 months aged children in Abobo District, Gambela regions southwest Ethiopia.
Method: A community-based cross-sectional study was employed in 436 pairs of mothers to children aged 12–23 months from 12 marches---27 April 2019. The study participant was recruited by multistage-sampling were used for each kebele. Data were entered into Epi-Data version 3.1 after cleaning and coded, exported to STATA/SE-14/R logistic regression analysis. Variables with P-value <0.25 in bivariate logistic regression were transported into multivariable logistic regression. A variable with 95%CI in AOR was used as claim predictors for the dropout rate.
Results: The overall dropout rate of immunization from completion was found 25.8% (95%CI: 21.5--30.2). Factors like mothers did not attend ANC (AOR= 4.59, 95% CI: 2.58, 7.84), being home delivery (AOR=6.46, 95% CI: (3.5--- 11.4), postponed last immunization scheduled (AOR=3.44, 95% CI: 1.98---5.97), children ill during measles vaccine (AOR=1.83, 95% CI: (1.02---3.28), Mothers refused ≥30 minutes for vaccine service waiting (AOR=3.58, 95% CI: (1.99, 6.44) were significantly associated with immunization dropout out.
Conclusion: The immunization dropout rate was unacceptable and higher compared to WHO reference (<10%). Home delivery postponed measles vaccine, child illness, ANC status Service refusal ≥30 minutes waiting for the vaccine were independently associated with dropout.
The Community-Based Child Health Integrated Program in Iran: A Mixed-Methods Process Evaluation
Authors: Barzegari Maliheh , Fathollahi Pourya , Afsharpour Fatemeh , Foroumandi Elaheh 
Published: March 2022

Objectives: The community-based Child Health Integrated Program (CHIP) was established to improve children’s health status in Iran. The current study was performed to understand how this program was implemented and experienced by the care providers and target group.
Methods: A total number of 249 mothers who had children under 5 years (clients) and 70 caregivers (providers) were selected from 42 health care centers of Tabriz city to participate in the study. Quantitative and qualitative data were collected using two separated semi-structured interviews as well as self-developed questionnaires. The mixed-method process evaluation study was examined and reported the exposure and satisfaction status of the clients, and reach, delivery, fidelity rates, and contextual factors toward the CHIP.
Results: Overall, low reach (11.2%), moderate-exposure (62.6%), and high satisfaction (80.1%) rates of the clients were reported to the program. The fidelity rate of the program tools was 42.9%, considered as an inadequate rate. Anthropometric measurement and vaccination of the children, as well as face-to-face training sessions for the mothers, were well delivered. However, some parts of the program including follow-up and group training sessions were delivered poorly.
Conclusions: This process evaluation study demonstrated that the CHIP is a promising intervention for improving children's health care. However, the barriers identified in this study warrant consideration in subsequent health care needs among children. Further research is required to identify ways to improve the implementation and delivery of this intervention.
Practice Implications: There is a dire need to enter some audit and feedback strategies in the form of monthly tracking of process indicators to extent of implementation of intervention components.
«Sabiats que us diray en riman». Prime considerazioni sul rapporto tra forma, comunicazione e pubblico nella Lògica del Gatzell di Ramon Llull
Authors: Staccioli Letizia 
Published: March 2022

La Lògica del Gatzell, primo esempio della produzione in volgare di Ramon Llull, è un compendio in versi basato sull’opera di al-Ghazali Maqasid al-falasifah e sul Tractatus di Pietro Ispano, cui il Beato aggiunse alcuni elementi del suo personale sistema di pensiero. Insieme alla versione latina, il Compendium logicae Algazelis, evidenzia l’intenzione di Llull di promuovere lo studio dei principi fondamentali della filosofia proponendo due diversi sussidi didattici, ciascuno pensato per un determinato genere di pubblico. Il compendio rimato era indirizzato a coloro che non conoscevano il latino e per questo l’autore ricorse a una forma letteraria e a una strategia comunicativa tipiche della tradizione romanza. Dall’analisi formale e retorica dell’opera, anche attraverso il confronto con la fonte araba e con il Compendium latino, emergono dettagli utili a individuare alcune delle caratteristiche dei possibili destinatari.
Innate lymphoid cells and COVID-19 severity in SARS-CoV-2 infection
Authors: Silverstein Noah J , Wang Yetao , Manickas-Hill Zachary , Carbone Claudia , Dauphin Ann , Boribong Brittany P , Loiselle Maggie , Davis Jameson , Leonard Maureen M , Kuri-Cervantes Leticia ,   MGH COVID-19 Collection & Processing Team, Meyer Nuala J , Betts Michael R , Li Jonathan Z , Walker Bruce D , Yu Xu G , Yonker Lael M , Luban Jeremy 
Published: March 2022

Background:Risk of severe COVID-19 increases with age, is greater in males, and is associated with lymphopenia, but not with higher burden of SARS-CoV-2. It is unknown whether effects of age and sex on abundance of specific lymphoid subsets explain these correlations.Methods:Multiple regression was used to determine the relationship between abundance of specific blood lymphoid cell types, age, sex, requirement for hospitalization, duration of hospitalization, and elevation of blood markers of systemic inflammation, in adults hospitalized for severe COVID-19 (n = 40), treated for COVID-19 as outpatients (n = 51), and in uninfected controls (n = 86), as well as in children with COVID-19 (n = 19), recovering from COVID-19 (n = 14), MIS-C (n = 11), recovering from MIS-C (n = 7), and pediatric controls (n = 17).Results:This observational study found that the abundance of innate lymphoid cells (ILCs) decreases more than 7-fold over the human lifespan – T cell subsets decrease less than 2-fold – and is lower in males than in females. After accounting for effects of age and sex, ILCs, but not T cells, were lower in adults hospitalized with COVID-19, independent of lymphopenia. Among SARS-CoV-2-infected adults, the abundance of ILCs, but not of T cells, correlated inversely with odds and duration of hospitalization, and with severity of inflammation. ILCs were also uniquely decreased in pediatric COVID-19 and the numbers of these cells did not recover during follow-up. In contrast, children with MIS-C had depletion of both ILCs and T cells, and both cell types increased during follow-up. In both pediatric COVID-19 and MIS-C, ILC abundance correlated inversely with inflammation. Blood ILC mRNA and phenotype tracked closely with ILCs from lung. Importantly, blood ILCs produced amphiregulin, a protein implicated in disease tolerance and tissue homeostasis. Among controls, the percentage of ILCs that produced amphiregulin was higher in females than in males, and people hospitalized with COVID-19 had a lower percentage of ILCs that produced amphiregulin than did controls.Conclusions:These results suggest that, by promoting disease tolerance, homeostatic ILCs decrease morbidity and mortality associated with SARS-CoV-2 infection, and that lower ILC abundance contributes to increased COVID-19 severity with age and in males.Funding:This work was supported in part by the Massachusetts Consortium for Pathogen Readiness and NIH grants R37AI147868, R01AI148784, F30HD100110, 5K08HL143183.
The Significance of the preoperative lactate dehydrogenase/albumin Ratio in the Prognosis of Colon Cancer: a retrospective study
Authors: Xie Zhihui , Zhou Hui , Wang Lei , Wu Yibo 
Published: March 2022

Background
We explored the relationship between the platelet-lymphocyte ratio (PLR), the prognostic nutritional index (PNI), the lactate dehydrogenase-albumin ratio (LDH/albumin ratio; LAR), the controlling nutritional status (CONUT) score, and the long-term survival of colon cancer patients.

Methods
We conducted a retrospective analysis of the clinical data and follow-up materials of 126 patients with colon cancer who underwent surgical treatment in the Department of Gastrointestinal Surgery of Jiangnan University Affiliated Hospital from June 2012 to December 2015. The receiver operating characteristic curve (ROC) was used to distinguish the high ratio group from the low ratio group. The Kaplan Meier method was used to draw the survival curve in our survival analysis. The log rank test was used for the univariate analysis and the Cox multivariate regression analysis was used to analyze the correlation between preoperative PLR, PNI, LAR, conut scores, and overall survival (OS) and progression free survival (PFS) of patients with colon cancer.

Results
The median follow-up time was 72 months. The OS rates at 3 and 5 years were 83.3% and 78.5%, respectively. The PFS rates at 3 and 5 years were 79.3% and 77.6%, respectively. The 3-year and 5-year OS rates in the low LAR group (≤4.91) were 90.9% and 87.1%, respectively, and were 56.0% and 44.0% in the high LAR group (>4.91) respectively. Univariate and multivariate analyses showed that the LAR value was correlated with OS and PFS (P < 0.05).

Conclusion
A high preoperative LAR is an independent predictor of the prognosis of colon cancer patients.
Comparison and correlation of cervical proprioception and muscle endurance in general joint hypermobility participants with and without non-specific neck pain—a cross-sectional study
Authors: Reddy Ravi Shankar , Tedla Jaya Shanker , Alshahrani Mastour Saeed , Asiri Faisal , Kakaraparthi Venkata Nagaraj 
Published: March 2022

Background
Cervical proprioception and muscle endurance are essential for maintaining cervical functional joint stability. Proprioception and muscle endurance may be impaired in those with general joint hypermobility (GJH). Examining these aspects is crucial. This study’s aims are to (1) compare the cervical joint position error (JPE) and muscle endurance holding capacities in GJH individuals with and without non-specific neck pain (NSNP) (2) to assess the relationship between hypermobility Beighton scores, cervical JPE’s, and muscle endurance in GJH individuals with and without NSNP.

Methods
In this cross-sectional comparative study, 33 GJH participants with NSNP (mean age 21.7 ± 1.8 years) and 35 asymptomatic participants GJH (mean age 22.42 ± 1.7 years) participated. Beighton’s score of ≥4 of 9 tests was used as criteria to diagnose GJH. Cervical JPEs were estimated in degrees using a cervical range of motion device, and muscle endurance (flexor and extensor) were estimated in seconds using a stopwatch.

Results
GJH participants with NSNP showed significantly larger cervical JPEs (p < 0.001) and decreased muscle endurance holding times (p < 0.001) compared to asymptomatic participants. Beighton hypermobility scores showed a significant moderate positive correlation with cervical JPEs (flexion: r = 0.43, p = 0.013), left rotation: r = 0.47, p = 0.005, right rotation: r = 0.57, p = 0.001) in NSNP individuals. Also, Beighton hypermobility scores showed a moderate negative correlation with muscle endurance in NSNP (flexor muscles: r = −0.40, p = 0.020, extensor muscles: r = −0.41, p = 0.020, and asymptomatic individuals (flexor muscles: −0.34, p = 0.045, extensor muscles: r = −0.45, p = 0.007).

Conclusion
GJH individuals with NSNP showed increased cervical JPEs and reduced muscle endurance compared to asymptomatic. Individuals with GJH with higher Beighton scores demonstrated increased cervical JPEs and reduced neck muscle endurance holding ability. In clinical practice, therapists should be aware of these findings, incorporate proprioceptive and muscle endurance assessments, and formulate rehabilitation strategies for NSNP individuals with GJM.
A Comparison of the Chemical Composition of Cooked Maize and Beans Left-over-water: Potentials as Food Drinks and Fertilizers
Authors: Jean Christophe Fopoussi Tuebue 
Published: March 2022

The paper highlighted the chemical characteristics of broth from cooked maize and compared them with that from cooked beans. Left-over-water from boiled maize were produced by cooking 2kg of a variety of Zea mays L. with 8kg of water. After this stage, samples of those solutions and samples of the water used for the cooking process were collected for laboratory analysis. The IonPac CS12A and IonPac AS12A analytical columns were respectively used for the separation of ammonium (NH4+), calcium (Ca2+), magnesium (Mg2+), sodium (Na+), and potassium (K+) as far as cations were concerned, and chloride (Cl) and sulfate (SO42-) in the case of anions. The measurements of pH and electric conductivity of fluids were respectively done with a pH-meter on the one hand and a conductivimetre on the other hand. Solutions from cooked maize are rich in mineral salts, particularly major macro elements (N and K) and minor macro elements (Ca, S, Mg). Concerning the third major macro element, notably the phosphorous, it is present in low amounts. The advantage of this fluid consists in its low electric conductivity. This fluid has a pH of 6.15. It is made of about 92% of water. Left-over-water from cooked maize and from cooked beans have very close chemical characteristics. In fact, these two fluids are rich in nitrogen and potassium, and mainly made of water. But, in detail, some particularities are present. The end-of cooking solutions of maize have higher amounts of sodium and chlorides, this joined with a higher electric conductivity. Concerning solutions from cooked beans, they have higher amounts of the different macro elements, and a lower electric conductivity. The recycling of end-of cooking solutions of maize must gainfully become for the nutritionists a favorable target for the future, and this due to the numerous nutrients contained. According however to the results obtained in the case of the present studies, the left-over-water from cooked maize can be gainfully use as fertilizers. Using it as manure requires an earthing-up directly after its application in other to avoid the loss of sulfur and nitrogen through gas emanation. Consuming solutions form cooked maize as herbal tea could be an excellent way to recycle the nutrients that have diffused from the seeds during the cooking process.
Studies on Correlation and Path Coefficient for Direct Selection between Pair of Traits Using Green Fruit Yield as Dependent Characters in Okra
Authors: Pundir Shivaji , Singh Manoj Kumar , Kumar Mukesh , Lodhi S. K. , Singh Atar , Alam Khursheed 
Published: March 2022

The study was carried out at the Horticulture Research Centre, Sardar Vallabhbhai Patel University of Agriculture & Technology, Modipuram, Meerut (U.P.). The fourteen quantitative traits, thirty genotypes of okra were tested in a Randomized Block Design (RBD) with three replications during the summer season of 2021. Fruit yield per plant showed highly significant and positive correlation with plant height, fruit diameter, number of branches per plant, fruit length, number of flowers per plant, test weight and number of fruits per plant at both genotypic and phenotypic level, indicating mutual association of these characters. Path coefficient analysis exhibits positive direct effect was observed towards fruit yield per plant for number of branches per plant followed by number of fruits per plant, days to first fruit harvesting, days to first flower initiation, fruit diameter, plant height, days to first fruit set and duration of crop at genotypic level. At the phenotypic level also the estimates of direct and indirect were generally less than genotypic level. The magnitudes of residual effects were found to be small at both the phenotypic and genotypic levels. These traits could be utilized in a selection procedure for okra crop enhancement in the future.
Management of Long Bone Fractures Using Diverse Fracture Fixation Techniques in Small Animals
Authors: Sharma Rohit Kumar , Mishra Saloni , Sharma Manish , Kumar Bishnoi Anil 
Published: March 2022

Total 11-cases of different breeds of dogs and one case of Persian cat having long bone fractures were successfully managed using totally intravenous anaesthesia (TIVA). For TIVA, combination of xylazine and ketamine were used for induction whereas sole ketamine was used for maintenance and atropine sulphate was used as preanaesthetic and anticholinergic agent. TIVA found suitable for orthopaedic surgeries in this study. Intramedullary pinning, bone-plating (Dynamic compression plating and Reconstruction plating), external skeletal fixation system (using jess clamps and epoxy) or combination of two different fracture fixation techniques were performed for surgical management of presented fracture cases. For combination of two orthopaedic techniques, either bone plating with supplementary IM-pinning or ESF with IM-pinning were used in this study. All cases were evaluated by radiographs as well as clinical performance of operated limbs. Radiographs were taken at regular interval for assessment of radiographic bone healing and in those cases where radiographs were not possible owner contacted telephonically.