bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gender-Difference Association Between Blood Pressure And Osteoporosis: Results From Henan Rural Cohort Study
Authors: Yi Xianhong , Tian Huiling , Chen Xinwei , Kang Ning , Liu Xiaotian , Wang Chongjian , Pan Jun 
Published: March 2022

Abstract
Purpose: Accumulating researches have observed the potential association between osteoporosis and hypertension, however, the evidence regarding gender-difference association between divergent proxies of blood pressure and osteoporosis was limited in rural areas.Methods: A total of 7689 participants enrolled from Henan Rural Cohort study were included in this research. Four proxies (systolic blood pressure (SBP); diastolic blood pressure (DBP); mean arterial pressure (MAP); pulse pressure (PP)) of blood pressure were measured. The association between blood pressure and osteoporosis was assessed by binary logistic regression with adjustment of potential influencing factors. All analyses were stratified by gender. To test the robust of results, similar analyses were also conducted in non-hypertension participants and postmenopausal women. Results: The significantly positive association between hypertension and osteoporosis was observed among women but not in men. Increasing blood pressure corresponded with prevalent osteoporosis among women, in which each 10 mmHg increase in SBP, MAP, and PP corresponded with 1.07-fold (1.03, 1.12), 1.14-fold (1.07, 1.21), and 1.08-fold (1.02, 1.15) prevalence of osteoporosis, respectively. This positive association was still significant among non-hypertension women and postmenopausal women, but not in pre-menopausal women.Conclusion: In rural areas, there was a positive association between blood pressure and prevalent osteoporosis in women, but not in men. Menopausal status may be responsible for this gender-difference.
Establishment and Characterization of An HER2-enriched Canine Mammary Cancerous Myoepithelial Cell Line with Stem Cell Properties
Authors: Chen Aolei , Ye Shaotang , Zheng Jiahui , Li Jichao , Chen Zejia , Zhang Yashan , Li Shoujun 
Published: March 2022

Abstract
Background
Canine mammary tumors (CMTs) have a poor prognosis, along with tumor recurrence and metastasis. Cell lines and organoids are vital in vitro models for CMT research. Although anti-HER2 therapy improves the patients’ outcome, anti-HER2 drug resistance is still a big challenge and yet no canine HER2-enriched cell line has been established. This study aimed at establishing such a novel canine mammary cell line. CMT-1 cell line was obtained from clinical tumor CMT-1 and characterized molecularly through qPCR, western blotting, immunochemistry, immunofluorescence. Its doubling time, migration rate and stemness were evaluated using growth study, wound healing assay and 3D cultures respectively. To determine its tumorigenesis, xenograft transplantation was performed.
Results
CMT-1 is a mixed canine mammary carcinoma that stains negative for estrogen receptors (ER) and progesterone receptors (PR), but positive for human epidermal growth receptor-2 (HER2), defined as HER2-enriched subtype. In this study, a CMT-1 cell line obtained from CMT-1 tumor was confirmed as myoepithelial cells and was successfully cultured for more than 50 passages showing the same immunoreactivity to ER, PR, and HER2. The doubling time of CMT-1 cell line was 26.67 h. A spontaneous epithelial to mesenchymal transition (EMT) was noticed during cell cultures. EMT-induced CMT-1 cells showed a faster migration rate compared to the original CMT-1 cells, but no significance was detected. CMT-1 cells grew on a 3D culture and formed grape-like, solid, and cystic mammosphere at different stages, indicating CMT-1 cell line has stem cell property. Inoculation of CMT-1 cells induced a mixed HER2-enriched mammary tumor with metastasis in mice.
Conclusions
A canine cancerous HER2-enriched myoepithelial cell line was successfully established and the first canine mammosphere was documented in this study. We are expecting this novel cell line and mammosphere could be used as a model for CMT tumorigenesis and new anti-HER2 drug screening in the future.
Thyroid Hormone Receptor β1 Suppresses Atypical Meningioma Proliferation in Vitro
Authors: zhang shen , Zhou Feihui , Pei Jingchun , Zhang Xinkui , Liu Chang , Li Zhigao , Mu Linjie , Wang Congqian , Liu Yue , Tang Zhiwei 
Published: March 2022

Abstract
Objective
To study the role of TRβ1 in atypical meningioma.
Methods
Forty-four meningioma specimens were collected, including 38 cases of WHO grade I and 6 cases of WHO grade II. The Ki67 index and TRβ1 mRNA level were detected in 44 cases. TRβ1 protein expression was evaluated in the meningiomas by IHC and Western blot analysis. Primary meningioma cells were cultured and identified with EMA and vimentin antibodies. Cell proliferation was evaluated using MTT, EdU and SRB assays. siRNA was used to silence TRβ1 expression. Western blotting assays were employed to detect p-ERK expression.
Results
There was a negative correlation between TRβ1 mRNA and the Ki67 index in meningioma tissues. The expression of TRβ1 protein in atypical meningiomas was lower than that in benign meningiomas (p < 0.05). T3 suppressed meningioma cell proliferation and cell survival in a dose-dependent manner. Moreover, we found that T3 significantly inhibited p-ERK protein expression (p < 0.05). Furthermore, TRβ1 knockdown almost completely rescued the T3-induced suppression of meningioma cell proliferation and partially rescued the T3-induced ERK activation.
Conclusion
Our study is the first to report that TRβ1 suppresses atypical meningioma cell proliferation by attenuating ERK phosphorylation.
Smooth muscle cell Piezo1 modulates small bowel contractility
Authors: Bautista Geoanna , Du Yingjie , Martin Nicolle , Matthews Michael , Nguyen Nam Phuong , Tokhtaeva Elmira , Solorzano-Vargas R. , Lewis Michael , Stelzner Matthias , He Ximin , Dunn James , Martin Martin 
Published: March 2022

Abstract
Piezo1 is a mechanosensitive cation channel expressed in the intestine's smooth muscle cells (SMCs), where its role has not been investigated. We used diverse methods to determine whether Piezo1 in murine SMCs is required to mediate myogenic contractility at rest and following stretch. Using an inducible system to deplete Piezo1 from SMCs led to impaired growth and delayed motility. The Piezo1 deficient SMCs had decreased contractile strength that was asynchronous, dysrhythmic, and less frequent compared to control samples at baseline and following stretch. Moreover, ablation of SMC Piezo1 led to thinning of the muscularis, changes in the cellular composition of the epithelial layer, and a decline in pacemaker cells within the muscularis, among other changes. Our data suggest that Piezo1 in the SMCs of the small bowel is required for modulating myogenic contractile strength and rhythmicity and maintaining cell types needed to promote motility.
Surface roughness prediction and optimization in the REMF process using an integrated DBN-GA approach
Authors: Lee Junghee - , Seo Yun-Su - , Kwak Jae-Seob 
Published: March 2022

Abstract
Surface roughness is a crucial factor affecting the surface quality of workpieces in the manufacturing industries. Thus, it is important to provide an accurate performance of surface roughness prediction and optimal parameters to reduce the burden of time and costs during the process. In this study, two predict models, namely multiple linear regression and deep belief network(DBN) models, were performed to accurately predict change in surface roughness in the rotational-electro magnetic finishing(REMF). Compared to the statistical-based model, the data-driven model based on the DBN architecture was a significantly considerable effect on surface roughness prediction in the REMF process. Among the considered DBN models, DBN5 architecture as [7, 14, 14, 1] showed effective features of the non-linear relationship between process parameters and response with the highest determination coefficient(R2) of 0.9340 and the lowest mean squared error(MSE) of 1.3037\(\times\)10-3 in the testing datasets. In addition, a genetic algorithm(GA) as a heuristic optimization technique was adopted to optimize the input parameters of the best derived DBN model. As a result, it proved that the DBN model integrated GA was able to be adopted for the accurate prediction of surface roughness and process optimization.
Decoding Motor Imagery of three-dimensional Random Movements Using Electrocorticographic Signals in Acute Training Setting
Authors: Chung Chun Kee , Yang Yu Jin , Ryun Seokyun , Kim June Sic 
Published: March 2022

Abstract
Background: Decoding movement-related signals from widespread brain areas may be advantageous given that diverse brain areas are activated for complex movements and imagined movements. Electrocorticography (ECoG) is a beneficial option in that it covers wide brain areas with a high signal-to-noise ratio. Here, we used ECoG to decode human reach-and-grasp movements and imagined movements. Method: Twenty-two epileptic patients with intracranial electrodes were asked to execute and imagine reach-and-grasp movements toward random targets with training periods of 8.2± 3.4 days. A multiple linear regression algorithm was used to estimate the offline prediction of the movement and imagined movement trajectories.Result: The three-dimensional reaching trajectories were decoded in motor execution (ME) and motor imagery (MI) tasks. The mean correlation coefficients for the x-axis, y-axis, and z-axis were correspondingly 0.5, 0.53, and 0.51 for the ME and 0.37, 0.19, and 0.28 for the MI. Conclusion: MI could be decoded in subjects who were acutely trained with acceptable performance when given a random target presentation task. ECoG brain-machine interface (BMI) given its wide coverage may be practical for decoding movements with multiple degrees of freedom in humans.
A Novel Potentially Toxic Cyanobacterial Species From the Genus Desmonostoc, Desmonostoc Alborizicum sp. nov., Iisolated From a Water Supply System of IranA
Authors: Nowruzi Bahareh , Becerra-Absalón Itzel 
Published: March 2022

Abstract
A cyanobacterial mat colonizing the wall of a qanat was found to be responsible for damages affecting the passage of water through the water supply system in Golestan province, Gorgan city, Iran. A qanat or kariz is a slightly sloping underground aqueduct used to transport water from water wells or aquifers to the surface for irrigation and drinking supply. A cyanobacterial strain was isolated from the mat and grown in BG11 liquid medium. Fragments of 16S rRNA, mcyG and mcyD genes were amplified and sequenced, as well as the 16S-23S internal transcribed spacer (ITS), from which secondary structures were analyzed. The obtained molecular data, together with morphological and physiological observations, were used to describe the new organism. The isolate was related to a morphotype of Nostoc sensu lato group, with similar characteristics of Desmonostoc, after microscopic inspections. The 16S rRNA phylogenetic analysis placed the isolate into the typical cluster of the recently proposed genus Desmonostoc. Morphological analysis revealed distinctive characteristic and secondary structures of 16S–23S rRNA (D1-D1´ and Box-B regions) derived from comparative analysis, which did not match known species of Desmonostoc. These results lead us to propose a novel Desmonostoc species, Desmonostoc alborizicum, which was described and compared with similar taxa. Furthermore, for the first time a potential toxic species of Desmonostoc was isolated from water supply, since the mcyD and mcyG genes of microcystin synthetase (mcy) cluster was successfully sequenced. The presence of the microcystin was confirmed by chemical analyses that also lead to the discovery of numerous other cyanobacterial secondary metabolites. We recognized substantial amounts of the potent microcystin variant microcystin-LR present in cell extracts of the Desmonostoc strain. Our findings contribute to a deeper understanding of diversity, systematics, and occurrence of Desmonostoc genus.
An Antioxidative Sophora Exosomes Encapsulated Hydrogel Promotes Spinal Cord Repair by Regulating Oxidative Stress Microenvironment
Authors: Chen Jiachen , Wu Jiahe , Mu Jiafu , Li Liming , Hu Jingyi , Cao Jian , Gao Jianqing , Lin Hangjuan 
Published: March 2022

Abstract

Background: Spinal cord injury (SCI) is recognized as a severe traumatic disease because of its complications and multiorgan dysfunction. After the injury, microenvironment stability disruption in the lesion will rapidly demolish the surrounding healthy tissues and cells via various pathways. Regulating the microenvironment is an effective strategy to mitigate the rigorous condition, which is beneficial for subsequent neural restoration and functional recovery. Nevertheless, sustained release, cellular uptake, and long-term retention in the impaired site of therapeutic molecules are insufficient.Results: Herein, a local-implantation system is constructed for SCI treatment by encapsulating exosomes derived from Flos Sophorae Immaturus (so-exos) in a polydopamine (pDA) modified hydrogel (pDA-Gel). So-exos are applied as nano-scale natural vehicles of rutin, a flavonoid phytochemical that has been confirmed effective in microenvironment mitigation and nerve regeneration. pDA-Gel not only enables the long-term retention and sustainable release of so-exos in the lesion but also offers a nutritional and suitable microenvironment for nerve regeneration at the same time.Conclusion: The pDA-Gel encapsulating so-exos exhibits rapid restoration of the nervous system and preservation of downstream urinary system by modulating the inflammatory and oxidative condition. The oxidative sophora exosome encapsulated hydrogel exhibits a potential SCI treatment via plant exosomes combinational delivery.
Crater wear prediction in turning Ti6Al4V considering cutting temperature effect
Authors: Zhuang Kejia , Li Ming , Lin Fengtian , Hu Cheng , Weng Jian , Gao Zhongmei , Wu Chaoqun 
Published: March 2022

Abstract
Tool wear has great impacts on tool life and cutting parameter selection in the machining process. As ceramic inserts are widely employed in efficient machining, it is of great significance to predict accurately the wear of ceramic tools. Crater wear is one of the most significant tool wear modes when turning difficult-to-cut materials at high speeds. The purpose of this research is to demonstrate the forming and influence mechanisms of crater wear in Ti6Al4V turning with ceramic inserts while considering cutting temperature at the tool-chip interface. An effort is made to describe quantitatively the appearance of crater wear from the perspective of temperature distribution based on crater wear experiments under various cutting conditions. In order to obtain the cutting temperature distribution efficiently, an analytical temperature prediction model is used in this paper. Then a prediction model for crater wear depth and width considering the influence of the maximum temperature is developed. The maximum crater wear depth and width models are validated by a series of cutting experiments, and the outcomes prove that the proposed models are practical. Additionally, the influence of various cutting parameters on crater wear is discussed. The cutting speed has the greatest impact on crater wear, followed by the feed rate. Furthermore, this research can be used to improve cutting parameters for controlling crater wear.
Pediatric Critical Illness in Wisconsin during the Epoch of COVID-19 in 2020
Authors: Godsey Claire , Gabor Rachel , Oelstrom Matthew J. , Hagen Scott , Peterson Jennifer , Taylor John , Mikhailov Theresa 
Published: March 2022

Abstract
Background
We perceived profound changes in the frequency of and reasons for admissions to pediatric intensive care units (PICUs) during the advent of the COVID-19 pandemic in Wisconsin, and we hypothesized that the rates of total, scheduled, and respiratory viral admissions were lower during the first year of the pandemic than would have been predicted by historical admission data. We also hypothesized that the rates of other categories of PICU admissions were different than would have been predicted by historical data, reflecting broad changes in PICU utilization paradigms during the pandemic.
Methods
Using mean weekly admission rates with standard deviations and rate ratios with 95% confidence intervals, we compared all Wisconsin PICU admissions during the pandemic epoch in 2020 (COVID-2020) to admissions in seasonally-matched, growth-adjusted “no-COVID” forecasts generated by time series modeling from all Wisconsin PICU admissions in the previous 5 years.
Results
We studied 27,425 admissions with 294,577 associated diagnoses including 104 admissions associated with a COVID-19 diagnosis (4.3% of admissions in COVID-2020). Total admissions were 60 ± 9 week− 1 in COVID-2020 vs. 103 ± 4 forecasted, RR 0.63 (95% CI: 0.59–0.68), p < 0.001. Scheduled admissions were 17 ± 6 week− 1 in COVID-2020 vs. 28 ± 3 forecasted, RR 0.61 (95% CI: 0.55–0.67), p < 0.001. And respiratory viral admissions were 8 ± 5 week− 1 in COVID-2020 vs. 19 ± 9 forecasted, RR 0.40 (95% CI: 0.33–0.48), p < 0.001. Some admission categories experienced dramatic declines (e.g., respiratory/ENT) while others experienced less decline (e.g., injury/poisoning/adverse effects) or no significant change (e.g., diabetic ketoacidosis). While admission categories demonstrated variability in rate decline during COVID-2020, no category had significantly increased weekly admissions.
Conclusions
We quantify and describe the extent of PICU admission decline in the first year of COVID in Wisconsin, so informing health care staffing and service planning, decisions regarding pandemic mitigation, and other strategies to combat the evolving pandemic. Total, scheduled, and respiratory viral admissions declined by 37, 39, and 60%, respectively. About half the reduction in total admissions was from easily-predictable categories such as scheduled and respiratory viral admissions.
The psychological and subjective experience of catatonia
Authors: Zingela Zukiswa 
Published: March 2022

Abstract
Background
Catatonia is a severe psychomotor disorder that presents as abnormality of movement which may also be excessive or severely slowed. It often inhibits communication when protracted or severe. In this study we investigated the emotive and cognitive experience of patients with catatonia during a prevalence study in an acute mental health unit from August 2020 to September 2021.
Methods
Ethical approval was received from the Nelson Mandela University Human Research Committee and convenience sampling was undertaken to recruit participants, four to eight weeks after discharge, following admission for a catatonic episode. Participants were requested to describe their thoughts, feelings and behaviours during the catatonic episode, in their own words.
Results
Forty-four (18.3%) of 241 participants had catatonia. Thirty (68.2%) of the 44 participants with catatonia provided data on their experience during the catatonic episode. The dominant themes of thoughts, feelings, and behaviors centered around yearning for or missing loved ones, heightened fear, intense anxiety, negative affect, aggression, obedience and withdrawal.
Conclusions
These findings indicate that catatonia mirrors the fight, flight, freeze and fawn reaction induced by extreme fear, anxiety or distress. Suggested psychological interventions include supportive, behavioural and cognitive behavioural strategies.
Trial registration:
Not applicable
Genome-wide analysis of Milk vetch dwarf virus and related alphasatellites from garlic and aphid isolates from Shandong Province, China
Authors: Zhu Xiaoping , Sun Xiaohui , Xiao Li , Cheng Chao , Shi Zhaopeng , Sun Zuowen , Ma Yongli 
Published: March 2022

Abstract
In 2019 and 2020, symptoms of dwarfing, yellowing, and reddening were observed in garlic in the open fields of Shandong Province, China. Milk vetch dwarf virus (MDV) was detected from aphids and symptomatic garlic plants using polymerase chain reaction analysis. Furthermore, it was demonstrated that garlic is the natural host of MDV using the aphid transmission test. Rolling circle amplification was combined with specific primers to amplify the complete genomes of MDV and its related alphasatellites. In this study, the complete genome sequences of MDV and related alphasatellites were amplified from the garlic and aphid samples for the first time.
Effect of BNT162b2 booster dose on anti-SARS-CoV-2 spike trimeric IgG antibodies in seronegative individuals
Authors: Salvagno Gian Luca , Henry Brandon M. , Pighi Laura , De Nitto Simone , Gianfilippi Gianluca , Lippi Giuseppe 
Published: March 2022

Abstract
Background: We provide here an updated analysis of an ongoing serosurveillance study, presenting data on the effect of a third dose of Pfizer/BioNTech BNT162b2 vaccine on serum anti-SARS-CoV-2 IgG antibodies.Methods: We tested baseline SARS-CoV-2 seronegative healthcare workers undergoing primary vaccination with the mRNA-based COVID-19 Comirnaty vaccine, followed by administration of homologous vaccine booster (third dose). Venous blood was collected before either dose of primary vaccination, at 1, 3 and 6 months afterwards, as well as before and 1 month after receiving the booster. The serum concentration of anti-SARS-CoV-2 spike trimeric IgG was assayed with DiaSorin Trimeric spike IgG immunoassay.Results: The final study population included 53 SARS-CoV-2 seronegative healthcare workers (median age 46 years; 60% females). A first peak of anti-SARS-CoV-2 spike trimeric IgG values was reached 1 month after completing primary vaccination, after which the levels gradually declined until before receiving the vaccine booster. A second peak of anti-SARS-CoV-2 spike trimeric IgG concentration was observed 1 month after receiving the vaccine booster dose (8700 kBAU/L), which was 39-fold higher than before receiving the vaccine booster (221 kBAU/L; p<0.001), but was also nearly 3-fold higher compared to values seen at the first peak (2990 kBAU/L; p<0.001). The rate of subjects with protective anti-SARS-CoV-2 spike trimeric IgG values (i.e., >264 kBUA/L) increased from 47.2% to 100% after 1 month from vaccine booster.Conclusion: These results support current policies fostering COVID-19 vaccine boosters to reinforce humoral immunity against SARS-CoV-2.