bluuebunny/crossref_metadata_2025_split huggingface dataset:
The regulatory effect of miR-181c-5p on the differentiation function of bone marrow mesenchymal stem cells in postmenopausal osteoporotic mice
Authors: Yang Chi , Shi Kai , Wang Jie , Yan Lianqi 
Published: April 2022

Abstract
Background
Osteoporosis (OP) is a metabolic bone disease syndrome for which there is no good treatment. In this study, we investigated the expression changes of miR-181c-5p in osteoporosis-derived BMMSCs, and the role and molecular mechanism in the osteogenic-lipogenic differentiation of BMMSCs.
Methods
In this study, an OP mouse model was successfully established using the ovariectomy method, and osteoporotic-derived BMMSCs (O-BMMSCs) and sham-operated-derived BMMSCs (S-BMMSCs) were isolated and cultured using the whole bone marrow method. A genetic screen revealed that miR-181c-5p was differentially expressed in O-BMMSCs and S-BMMSCs. The expression levels of miR-181c-5p in BMMSCs were overexpressed or inhibited by cell transfection, and the regulatory effects of miR-181c-5p on the proliferation and osteogenic-adipogenic differentiation of BMMSCs were examined using MTT, multi-directional differentiation induction, alizarin red staining, oil red O staining, qRT-PCR and Western blot. Candidate target genes for miR-181c-5p were screened by target gene prediction software and bioinformatics websites, and target gene validation was performed.
Results
The study found that overexpression of miR-181c-5p or inhibition of miR-181c-5p had no significant effect on the proliferation ability of BMMSCs. Upregulation of miR-181c-5p could reduce the osteogenic ability and enhance the adipogenic ability of BMMSCs, while downregulation of miR-181c-5p could increase the osteogenic ability and inhibit the adipogenic ability of BMMSCs. Besides, Foxo1 was confirmed as a direct target gene of miR-181c-5p, and miR-181c-5p negatively regulated Foxo1 expression. Downregulation of miR-181c-5p in O-BMMSCs promoted Foxo1 expression, improved the osteogenic differentiation of O-BMMSCs, and reduced abnormal lipogenic differentiation of O-BMMSCs and eventually partially restored the normal differentiation ability of O-BMMSCs.
Conclusion
miR-181c-5p regulated the osteogenic and adipogenic differentiation of BMMSCs by negatively regulating the expression of target gene Foxo1. The overexpression of miR-181c-5p in the process of osteoporosis leads to the disruption of the balance of osteogenic and adipogenic differentiation of BMMSCs, and reduces the bone formation ability of stem cells.
Estimation of plant density and plant height of winter wheat breeding material based on UAV digital images
Authors: zang hecang , Yanjing WANG , Xiuzhong YANG , Jia HE , Qing ZHAO , meng zhou , guoqing zheng , guoqiang li 
Published: April 2022

Abstract
【Aims】 In order to obtain the plant density and plant height information of winter wheat breeding material quickly and accurately, it was of great practical significance for the growth monitoring and yield prediction of winter wheat breeding material. 【Method】 In this study, a drone equipped with a digital camera was used to obtain the drone digital orthophoto (DOM) and digital surface model (DSM) of winter wheat breeding material at the seedling stage, jointing stage, booting stage, flowering stage and grain filling stage. The coverage of winter wheat was extracted from drone images, and the relationship between coverage and plant density was established; the plant height estimation models of winter wheat breeding material were established respectively at jointing, booting, flowering and grain filling stages. Based on the ground measurement of winter wheat breeding material height (H), the accuracy of plant height of winter wheat breeding material extracted by DSM was verified.【Result】 The results showed that the winter wheat coverage extracted based on the UAV images at the seedling stage had a high correlation with the measured plant density, and the R2 was 0.8205. The new winter wheat cultivar H extracted by DSM was significantly correlated with the measured H, and the fitted R2 and RMSE of the predicted plant height and the measured value were 0.9554 and 6.3233 cm, respectively. 【Conclusion】 The results indicated that the use of UAV aerial imagery to predict the plant density and plant height of winter wheat breeding material has good applicability, and can provide technical reference for future crop phenotype information monitoring.
The Anti-carbohydrate antibody repertoire in type 1 diabetes
Authors: Tran Paul , Dong Fran , Kin Eileen , Richardson Katherine , Tran Lynn , Waugh Kathleen , Hopkins Diane , Cummings Richard , Wang Peng George , Rewers Marian , She Jin-Xiong , Purohit Sharad 
Published: April 2022

Abstract
Type 1 diabetes (T1D) is an autoimmune disease, characterized by the presence of auto-antibodies to several protein and non-protein antigens. We report circulating levels of antibodies towards naturally occurring glycans using our customized glycan array and show that anti-carbohydrate antibodies (ACA) are associated with pathogenesis and progression to T1D. We compared circulatory levels of ACAs against 202 glycans in a cross-sectional cohort of T1D patients (n=278) and healthy controls (n=298), as well as in a longitudinal cohort (n=112). We identified 11 clusters of ACAs associated with glycan function class. Clusters enriched for aminoglycosides, blood group A and B antigens, glycolipids, ganglio-series, and O-linked glycans were associated with progression to T1D. ACAs against the aminoglycoside commonly used for neonatal sepsis, gentamicin, and its related structures, G418 and sisomicin, were associated with islet autoimmunity. ACAs improved discrimination of T1D status of individuals over a model with only clinical variables. ACAs are potential biomarkers for T1D.
Using Inga punctata in forest restoration influences the accumulation of tree soil carbon and nitrogen, and the nitrogen-fixer and lignin degrader bacterial community compositions, complexity and stability.
Authors: Eaton William , Hamilton Debra A. 
Published: April 2022

Abstract
AimsThis study was designed to determine if planting the leguminous tree Inga punctata in cleared former tropical premontane wet forest lands changed the Nitrogen (N)-fixer and Lignin Degrader bacterial community compositions, complexity and stability, to enhance the soil carbon (C) and nitrogen (N) components.MethodsSoils were collected from old growth, 4, 8 and 11-year-old I. punctata trees, and assessed for differences in various soil C and N metrics, and the mean of percent sequences (MPS, as “relative abundance” via Next Generation eDNA Sequencing), composition, stability and complexity of the community of N-fixer and Lignin Degrader genera.ResultsThe MPS N-fixer genera decreased and that of the Lignin Degraders increased over time, as did the composition and stability of both groups. There were negative correlations between the MPS levels of the N-fixer and Lignin Degrader genera, positive correlations between the MPS levels of Lignin Degraders, TN, NO3−, TOC, and Biomass C, negative correlations between the MPS levels of Lignin Degraders and NH4+, and opposite correlations between these C and N metrics and MPS levels of N-fixers.ConclusionsThis is the first evidence that I. punctata facilitates changes over time in the amount and composition of tree soil N-fixer and Lignin Degrader genera, enhances the stability and complexity of both groups, increases the accumulation of tree soil C and N, thus, increasing the potential for these soils to serve as C and N sinks, and supporting the future use of this reforestation management strategy.
Impact of COVID-19 Pandemic on Mental Health of Children: A Longitudinal Survey in the ABCD Study Cohort
Authors: Hamatani Sayo , Hiraoka Daiki , Makita Kai , Tomoda Akemi , Mizuno Yoshifumi 
Published: April 2022

Abstract
A large longitudinal study on the impact of the COVID-19 pandemic on mental health in children is limited. This large-scale longitudinal observational study examines the pandemic’s effects on children’s mental health while considering the effects of parental care styles. The Adolescent Brain Cognitive Development study is a large-scale, longitudinal multicenter study in the United States. Of the 11,875 children aged 9–12 years in its database, 4,885 subjects were selected for this study. The child behavior checklist and parental monitoring questionnaire (PMQ) were used to assess children’s mental health and parental support styles, respectively. Data collected before and during the pandemic were compared. Withdrawn/depressed and attention problems significantly worsened during compared to before the COVID-19 pandemic (p < 0.001, Withdrawn/depressed; 53.4 ± 5.7 to 53.7 ± 5.9, attention problems; 53.4 ± 5.4 to 53.6 ± 5.6). Simple slope analysis found withdrawn/depressed problems worsened when the PMQ was 1 SD below the mean, and rule-breaking behavior was improved when the PMQ was 1 SD above the mean. While the COVID-19 pandemic exacerbated children’s depressive symptoms and attention issues, the effects may be minor. Additionally, parental involvement behaviors serve as a protective factor for the child’s mental health even during the pandemic.
Life-course Inequalities in Intrinsic Capacity among Chinese Older Adults
Authors: Si Yafei , Hanewald Katja , Chen Shu , Li Bingqin , Bateman Hazel , Beard John 
Published: April 2022

Abstract
Background
Maintaining and optimising intrinsic capacity (IC) across a person’s life course is a core component of the World Health Organization’s model of healthy ageing. However, the contribution of cumulative health inequalities over time to subtle changes in IC in late life is not well understood.
Methods
We included 21,783 participants aged 45 + from the China Health and Retirement Longitudinal Study and calculated a validated prognostic value of IC. We included eleven early-life factors to investigate their direct influence on IC over thirty years later and cumulative influence through four current socioeconomic factors. We used multivariable linear regression and concentration index decomposition to investigate the contributions of each determinant to IC inequalities. Mediation analysis identified the direct and cumulative contribution of early-life factors.
Results
Participants with an advantaged environment in childhood and a higher current socioeconomic position had a significantly higher IC score. This inequality was greatest for cognitive capacity and sensory capacity. Overall, early-life factors directly explained 13.92% (95% CI: 12.07–15.77%) of IC inequalities, while 28.57% (95% CI: 28.19–28.95%) of IC inequalities were explained through the cumulative effects of socioeconomic inequalities over a person’s life course.
Conclusion
In China, unfavourable early-life factors appear to directly decrease late-life health status, particularly cognitive and sensory capacities rather than locomotor functioning, psychological capacity or homeostasis, and these effects are exacerbated by the cumulative socioeconomic inequalities over a person’s life course. Interventions in early life and subsequently across the life course may be effective in reducing these disparities.
Developmental disruption and restoration of brain synaptome architecture in the Pax6 neurodevelopmental disease model
Authors: Tomas-Roca Laura , Qiu Zhen , Fransén Erik , Gokhale Ragini , Bulovaite Edita , Price David , Komiyama Noboru , Grant Seth 
Published: April 2022

Abstract
Neurodevelopmental disorders of genetic origin delay the acquisition of normal abilities and cause disabling phenotypes. However, spontaneous attenuation and even complete amelioration of symptoms in early childhood and adolescence occur in many disorders, suggesting that brain circuits possess an intrinsic capacity to overcome some germline mutations. We examined the molecular composition of almost a trillion excitatory synapses on a brain-wide scale between birth and adulthood in mice carrying a mutation in the homeobox transcription factor Pax6, a neurodevelopmental disorder model. Pax6 haploinsufficiency had no impact on total synapse number at any age. By contrast, the molecular composition of excitatory synapses, the postnatal expansion of synapse diversity and the acquisition of normal synaptome architecture were delayed in all brain regions, interfering with network and cognitive functions. Specific excitatory synapse types and subtypes were affected in two key developmental age-windows. These phenotypes were reversed within 2-3 weeks of onset, restoring synapse diversity and synaptome architecture to the normal developmental trajectory. Synapse subtypes with rapid protein turnover mediated the synaptome remodeling. This brain-wide capacity for remodeling of synapse molecular composition to recover and maintain the developmental trajectory of synaptome architecture may help confer resilience to neurodevelopmental genetic disorders.
Study on the Equation Between Liver MRI T2* Values and Liver Iron Concentration in Thalassemia Patients
Authors: Xu Fengming , Yi Jixing , Liang Bumin , Luo Chaotian , Peng Fei , Peng Yuzhao , Peng Peng 
Published: April 2022

Abstract
Background: The liver iron concentration (LICF) provided by FerriScan is already certified. But there are some restrictive factors. Therefore, we explored the relationship curves of LICF and MRI liver T2 *, and constructed the equations for both. Methods: Liver MRI T2* values of 273 thalassemia patients were measured by CMRtools/Thalassemia Tools (CMRtools) and divided into test and verification groups. The T2* values of the test group and LICF were used to build the equation, through which the T2* values of the validation group were converted to the liver iron concentration（LICe）. The relationship between LICe and LICF was explored. According to the clinical liver iron concentration grading, LICF and LICe were grouped to explore the relationship between the them in the validation group. Results: The equation built by the test group was LICF=37.393T2*^(-1.22)（R2=0.971，P＜0.05）. There was no statistical difference between LICe and LICF in the validation group（P＞0.05）; There was significant consistency（W=0.991, P＜0.05）and significant correlation（rs=0.983，P＜0.05） between them .There was no statistical difference in the clinical grading between LICe and LICF in the validation group （P＞0.05）. There was significant consistency between the clinical grading results（K=0.943，P＜0.05. Conclusion: Through the equation LICF=37.393T2*^(-1.22), after measuring the liver T2* value, the liver iron concentration (LIC) equivalent to LICF can be accurately calculated.
TRPV3-ANO1 interaction positively regulates wound healing in keratinocytes
Authors: Yamanoi Yu , Takayama Yasunori , Hosogi Shigekuni , Marunaka Yoshinori , Tominaga Makoto 
Published: April 2022

Abstract
Transient receptor potential vanilloid 3 (TRPV3) belongs to the TRP ion channel super family and functions as a nonselective cation channel that is highly permeable to calcium. This channel is strongly expressed in skin keratinocytes and is involved in warmth sensation, itch, wound healing and secretion of several cytokines. Previous studies showed that anoctamin1 (ANO1), a calcium-activated chloride channel, was activated by calcium influx through TRPV1, TRPV4 or TRPA1 and that these channel interactions were important for TRP channel-mediated physiological functions. We found that ANO1 was expressed by normal human epidermal keratinocytes (NHEKs). We observed that ANO1 mediated currents upon TRPV3 activation of NHEKs. Using an in vitro wound-healing assay, we observed that either an ANO1 blocker or low chloride medium inhibited cell migration and proliferation through p38 phosphorylation, leading to cell cycle arrest. These results indicated that chloride influx through ANO1 activity enhanced wound healing by keratinocytes.
Gene Ontology informed neural network model for microbe functionality discovery in human diseases
Authors: Liu Yunjie , Zhang Yaozhong , Imoto Seiya 
Published: April 2022

Abstract
Background: The human microbiome plays a crucial role in human health and is associated with a number of human diseases. Determining microbiome functional roles in human diseases remains a biological challenge due to the high dimensionality of metagenome gene features. However, existing models were limited in providing biological interpretability, where the functional role of microbes in human diseases is unexplored. Here we propose to utilize a neural network-based model incorporating Gene Ontology (GO) relationship network to discover the microbe functionality in human diseases. Results: We prepare two benchmark datasets including diabetes and liver cirrhosis to explore the microbe functionality in human diseases. Our model discovered and visualized the novel candidates important microbiome genes and their functions by calculating the important score of each gene and GO term in the network in both datasets. Furthermore, we demonstrate that our model achieves a competitive performance in predicting the disease by comparison with other non-Gene Ontology informed models to ensure the importance of candidates genes and their functions. Conclusions: The discovered candidates important microbiome genes and their functions provide novel insights into microbe contribution in functional aspects.
Safety, Efficacy and Prognosis of Anticoagulant Therapy for Portal Vein Thrombosis in Cirrhosis: A Retrospective Cohort Study
Authors: Zhang Zhiqi , Zhao Ying , Li Dandan , Guo Mingxing , Li Hongyu , Liu Ranjia , Cui Xiangli 
Published: April 2022

Abstract
Background: The role of anticoagulants in the treatment of cirrhotic PVT remains controversial. This study aimed to analyze the safety and efficacy of anticoagulant therapy in patients with cirrhotic portal vein thrombosis (PVT) and its impact on prognosis.Methods: A retrospective cohort study was conducted for PVT patients with liver cirrhosis in our hospital. The primary outcome of the study was the PVT recanalization rate. Other outcomes included bleeding rate, liver function, and mortality. Cox and Logistic regression were used to explore the risk factors of outcomes. Results: This study included 77 patients that 27 patients in the anticoagulant group and 50 in the non-anticoagulant group. Anticoagulant therapy was associated with higher rate of PVT recanalization (44.4% vs 20.0%, log-rank P = 0.016) and lower rate of PVT progression (7.4% vs 30.0%, log-rank P = 0.026), and without increasing the rate of total bleeding (14.8% vs 24%, P = 0.343), major bleeding (3.7% vs 6%, P = 0.665) and variceal bleeding (3.7% vs 16%, P = 0.109). The safety and efficacy of different anticoagulants were similar. The Child-Pugh grade of the anticoagulant therapy group was better than that of the non-anticoagulant therapy group (P = 0.030). There was no significant difference in the 2-year survival rate of the two groups. Conclusion: Anticoagulants could increase the PVT recanalization rate and reduce the PVT progression rate without increasing the rate of bleeding. Anticoagulants may be beneficial to improving the liver function of patients with cirrhotic PVT. There was no significant difference in the safety and efficacy of different anticoagulants in the treatment of cirrhotic PVT
Melatonin ameliorates ovarian hyperstimulation syndrome (OHSS) through SESN2 regulated antiapoptosis
Authors: Zheng Min , Liu Mei , Zhang Cong 
Published: April 2022

Abstract
Research question: Ovarian hyperstimulation syndrome (OHSS) is one of the most severe complications after ovarian stimulation during assisted reproductive technology (ART). However, its pathogenesis still remains unclear. Melatonin is an important antioxidant factor in female reproduction and Sestrin-2 (SESN2) is reported to be involved in cellular response to different stress conditions. Whether or not melation and SESN2 are involved in OHSS is still a question to us clinicians. Design: We collected the granulosa cells of OHSS patients and focused on the role of SESN2 in OHSS. We also studied the role and mechanism of melatonin plays in OHSS patients. Results: We found that the expression of SESN2 was increased in the granulosa cells of OHSS patients (n=24) than those in controls (n=15). Incubation with angiotensin II (1μM, 2μM) in HUVECs and H2O2 (0.1mM, 0.2mM) in KGNs increased the generation of ROS concurrent with the increased expression of SESN2, while melatonin treatment partly restored SESN2 levels. Mechanism study demonstrated that SESN2 was deeply involved in the regulation of AMPK and mTOR, whearas melatonin partially restored angiotensin II or H2O2 induced the activation of AMPK phosphorylation and the inhibition of mTOR, 4EBP1 and S6K1 phosphorylation, all of which could trigger cell apoptosis. Additionally, we found that SESN2 knockdown and treatment with rapamycin (mTOR inhibitor) in HUVECs partly abolished the antiapoptosis effects of melatonin. Conclusions: These findings indicated that melatonin attenuated ROS-induced apoptosis through SESN2-AMPK-mTOR in OHSS. Thus, melatonin is likely to be a potential and important therapeutic agent for treating and preventing OHSS.
Survival analysis using the Covid-Death Mean-Imputation (CoDMI) algorithm: a first clinical application in radiation oncology
Authors: De Felice Francesca , Mazzoni Luca , Musio Daniela , Tombolini Vincenzo , Moriconi Franco 
Published: April 2022

Abstract
Purpose. We illustrate a clinical application of Covid-Death Mean-Imputation (CoDMI) algorithm in survival analysis.Material and methods. We analyzed 94 patients treated for primary locally advanced rectal cancer (LARC). Overall survival was calculated in months from diagnosis to first event (last follow-up/death). Because Covid-19 death events potentially bias survival estimation, to eliminate skewed data due to Covid-19 death events the observed lifetime of Covid-19 cases was replaced by its corresponding expected lifetime in absence of the Covid-19 event using CoDMI algorithm. In a traditional Kaplan-Meier approach, patient died of Covid-19 (DoC) can be: i) excluded to the cohort, or ii) counted as censored (Cen), or iii) considered as died of disease (DoD). CoDMI algorithm offers an additional, more satisfactory option: iv) DoC events are mean-imputed by the Kaplan-Meier estimator. With this approach, observed lifetime of each DoC patient is considered as an “incomplete data” and is extended by an additional expected lifetime computed using the classical Kaplan-Meier model. Results. 16 patients were DoD, 1 patient was DoC and 77 cases were Cen. The DoC patient died of Covid-19 52 months after diagnosis. CoDMI algorithm computed the expected future lifetime provided by the Kaplan-Meier estimator applied to the no-DoC observations as well as to the DoC data itself. Given the DoC event at 52 months, CoDMI algorithm estimated that this patient would be died after 79.5 months of follow-up.Conclusion. CoDMI algorithm leads to “unbiased” probability of overall survival in LARC patients with Covid-19 infection, compared with that provided by a naïve application of Kaplan-Meier approach. This allows for a proper interpretation/use of Covid-19 events in survival analysis. A user-friendly version of CoDMI is freely available at https://github.com/alef-innovation/codmi.