bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of bednets with dual insecticide-treated netting (Interceptor® G2) on side and roof panels against Anopheles arabiensis in north-eastern Tanzania
Authors: Mbewe Njelembo J. , Rowland Mark W. , Snetselaar Janneke , Azizi Salum , Small Graham , Nimmo Derric D. , Mosha Franklin W. 
Published: September 2022

Abstract
Background
Optimising insecticide use and managing insecticide resistance are important to sustain gains against malaria using long-lasting insecticidal nets (LLINs). Restricting insecticides to where mosquitoes are most likely to make multiple contacts could reduce the quantity of insecticide needed to treat the nets. Previous studies have shown that nets partially treated with a pyrethroid insecticide had equivalent mortality compared to a fully treated net. This study compared the efficacy of: (i) whole Interceptor® G2 nets (IG2; a dual-active LLIN containing alpha-cypermethrin and chlorfenapyr), (ii) nets with roof panels made of IG2 netting, (iii) nets with side panels made of IG2 netting and (iv) whole untreated nets as test nets.

Methods
The study was conducted in cow-baited experimental huts, Moshi Tanzania, using a four-arm Latin square design. Test nets had 30 holes cut in panels to simulate a typical net after 2–3 year use. The trial data were analysed using generalized linear models with mortality, blood-feeding, exophily and deterrence against wild free-flying Anopheles arabiensis as outcomes and test nets as predictors.

Results
Mortality was significantly higher in the nets with roof IG2 [27%, P = 0.001, odds ratio (OR) = 51.0, 95% CI = 4.8–546.2), side IG2 (44%, P < 0.001, OR = 137.6, 95% CI = 12.2–1553.2] and whole IG2 (53%, P < 0.001, OR = 223.0, 95% CI = 19.07–2606.0) nettings than the untreated (1%) nets. Mortality was also significantly higher in the whole IG2 net compared to the net with roof IG2 netting (P = 0.009, OR = 4.4, 95% CI = 1.4–13.3). Blood feeding was 22% in untreated, 10% in roof IG2, 14% in side IG2 and 19% in whole IG2 nets. Exiting was 92% in untreated, 89% in roof IG2, 97% in side IG2 and 94% whole IG2 nets.

Conclusion
The results show that although the roof-treated IG2 net induced greater mortality compared to untreated nets, its efficacy was reduced compared to whole IG2 nets. Therefore, there was no benefit to be gained from restricting dual-active ingredient IG2 netting to the roof of nets.

Graphical Abstract
A longitudinal study on quality of life along the spectrum of Alzheimer’s disease
Authors: Mank Arenda , Rijnhart Judith J. M. , van Maurik Ingrid S. , Jönsson Linus , Handels Ron , Bakker Els D. , Teunissen Charlotte E. , van Berckel Bart N. M. , van Harten Argonde C. , Berkhof Johannes , van der Flier Wiesje M. 
Published: September 2022

AbstractBackgroundQuality of life (QoL) is an important outcome from the perspective of patients and their caregivers, in both dementia and pre-dementia stages. Yet, little is known about the long-term changes in QoL over time. We aimed to compare the trajectories of QoL between amyloid-positive and amyloid-negative SCD or MCI patients and to evaluate QoL trajectories along the Alzheimer’s disease (AD) continuum of cognitively normal to dementia.MethodsWe included longitudinal data of 447 subjective cognitive decline (SCD), 276 mild cognitive impairment (MCI), and 417 AD dementia patients from the Amsterdam Dementia Cohort. We compared QoL trajectories (EQ-5D and visual analog scale (VAS)) between (1) amyloid-positive and amyloid-negative SCD or MCI patients and (2) amyloid-positive SCD, MCI, and dementia patients with linear mixed-effect models. The models were adjusted for age, sex, Charlson Comorbidity Index (CCI), education, and EQ-5D scale (3 or 5 level).ResultsIn SCD, amyloid-positive participants had a higher VAS at baseline but showed a steeper decline over time in EQ-5D and VAS than amyloid-negative participants. Also, in MCI, amyloid-positive patients had higher QoL at baseline but subsequently showed a steeper decline in QoL over time compared to amyloid-negative patients. When we compared amyloid-positive patients along the Alzheimer continuum, we found no difference between SCD, MCI, or dementia in baseline QoL, but QoL decreased at a faster rate in the dementia stage compared with the of SCD and MCI stages.ConclusionsQoL decreased at a faster rate over time in amyloid-positive SCD or MCI patients than amyloid-negative patients. QoL decreases over time along the entire AD continuum of SCD, MCI and dementia, with the strongest decrease in dementia patients. Knowledge of QoL trajectories is essential for the future evaluation of treatments in AD.
Patterns and drivers of tree carbon stocks in Kashmir Himalayan forests: implications for climate change mitigation
Authors: Dar Ashaq Ahmad , Parthasarathy Narayanaswamy 
Published: September 2022

AbstractBackgroundTemperate forests are major carbon sinks because of their high storage potential and low decomposition processes. We quantified tree carbon (TC) storage from 143 plots distributed across three major forest types of Kashmir Himalaya, relative to differences in ecological factors. Combined regression and Random Forest (RF) analysis were used to examine the distribution of TC stock along ecological gradients and recognize the role of driving factors on TC stocks.ResultsAmong the three forest types, sub-alpine (SA) forest was the primary TC sink, accounting for 228.73 t ha−1 of carbon, followed by mixed conifer (MC; 181.29 t C ha−1) and blue pine (BP; 133.04 t C ha−1) forests. The distribution of TC stocks among the three forest types differed significantly (χ2 = 18.87;P = 0.000). Relative carbon stock analysis demonstrated thatAbies pindrowandPinus wallichianaaccounted 91% of TC stocks across the landscape. Basal area, mean diameter at breast height (DBH), elevation, disturbance and precipitation had significant effects on TC stocks in bivariate regression models. The RF model explained 86% of the variation; basal area interpreted 30.15%, followed by mean DBH (17.96%), disturbance complex (10.64%), precipitation (8.00%) and elevation (7.34%).ConclusionsKashmir Himalayan forests are significant carbon sinks as they store a substantial quantum of carbon in trees. Forest carbon, an essential climatic indicator, is determined by a complex interaction of other ecological variables, particularly stand structural features. The study provides insights into the role of these natural forests in climate change mitigation and in REDD+/national commitments to offset the carbon.
Can information and communication technology and institutional quality help mitigate climate change in E7 economies? An environmental Kuznets curve extension
Authors: Gyamfi Bright Akwasi , Ampomah Asiedu B. , Bekun Festus V. , Asongu Simplice A. 
Published: September 2022

AbstractUnderstanding the role of information communication and technology (ICT) in environmental issues stemming from extensive energy consumption and carbon dioxide emission in the process of economic development is worthwhile both from policy and scholarly fronts. Motivated on this premise, the study contributes to the rising studies associated with the roles of economic growth, institutional quality and information and communication technology (ICT) have on CO2 emission in the framework of the 21st Conference of the Parties (COP21) on climate convention in Paris. Obtaining data from the emerging industrialized seven (E7) economies (China, India, Indonesia, Russia, Mexico, Brazil and Turkey) covering annual frequency from 1995 to 2016 for our analysis achieved significant outcome. From the empirical analysis, economic globalization and renewable energy consumption both reduce CO2 emissions while ICT, institutional quality and fossil fuel contribute to the degradation of the environment. This study affirms the presence of an environmental Kuznets curve (EKC) phenomenon which shows an invented U-shaped curve within the E7 economies. On the causality front, both income and its square have a feedback causal relationship with carbon emissions while economic globalization, institutional quality, ICT and clean energy all have a one-way directional causal relationship with CO2 emissions. Conclusively, the need to reduce environmental degradation activities should be pursued by the blocs such as tree planting activities to mitigate the effect of deforestation. Furthermore, the bloc should shift from the use of fossil-fuel and leverage on ICT to enhance the use of clean energy which is environmentally friendly.
CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer’s disease
Authors: Park Hanseul , Shin Jaein , Kim Yunkyung , Saito Takashi , Saido Takaomi C. , Kim Jongpil 
Published: September 2022

Abstract
Background
Aberrant DNA methylation patterns have been observed in neurodegenerative diseases, including Alzheimer's disease (AD), and dynamic changes in DNA methylation are closely associated with the onset and progression of these diseases. Particularly, hypomethylation of the amyloid precursor protein gene (APP) has been reported in patients with AD.

Methods
In this study, we used catalytically inactivated Cas9 (dCas9) fused with Dnmt3a for targeted DNA methylation of APP, and showed that the CRISPR/dCas9-Dnmt3a-mediated DNA methylation system could efficiently induce targeted DNA methylation of APP both in vivo and in vitro.

Results
We hypothesized that the targeted methylation of the APP promoter might rescue AD-related neuronal cell death by reducing APP mRNA expression. The cultured APP-KI mouse primary neurons exhibited an altered DNA-methylation pattern on the APP promoter after dCas9-Dnmt3a treatment. Likewise, the APP mRNA level was significantly reduced in the dCas9-Dnmt3a-treated wild-type and APP-KI mouse primary neurons. We also observed decreased amyloid-beta (Aβ) peptide level and Aβ42/40 ratio in the dCas9-Dnmt3a-treated APP-KI mouse neurons compared to the control APP-KI mouse neurons. In addition, neuronal cell death was significantly decreased in the dCas9-Dnmt3a-treated APP-KI mouse neurons. Furthermore, the in vivo methylation of APP in the brain via dCas9-Dnmt3a treatment altered Aβ plaques and attenuated cognitive and behavioral impairments in the APP-KI mouse model.

Conclusions
These results suggest that the targeted methylation of APP via dCas9-Dnmt3a treatment can be a potential therapeutic strategy for AD.
Multi-omic profiling of the leukemic microenvironment shows bone marrow interstitial fluid is distinct from peripheral blood plasma
Authors: Nierves Lorenz , Guo Jian , Chen Siyuan , Tsui Janice , Uzozie Anuli C. , Bush Jonathan W. , Huan Tao , Lange Philipp F. 
Published: September 2022

Abstract
Background
The bone marrow is the place of hematopoiesis with a microenvironment that supports lifelong maintenance of stem cells and high proliferation. It is not surprising that this environment is also favourable for malignant cells emerging in the bone marrow or metastasizing to it. While the cellular composition of the bone marrow microenvironment has been extensively studied, the extracellular matrix and interstitial fluid components have received little attention. Since the sinusoids connect the bone marrow interstitial fluid to the circulation, it is often considered to have the same composition as peripheral blood plasma. Stark differences in the cellular composition of the bone marrow and peripheral blood with different secretory capacities would however suggest profound differences.

Methods
In this study we set out to better define if and how the bone marrow interstitial fluid (BMIF) compares to the peripheral blood plasma (PBP) and how both are remodeled during chemotherapy. We applied a multi-omic strategy to quantify the metabolite, lipid and protein components as well as the proteolytic modification of proteins to gain a comprehensive understanding of the two compartments.

Results
We found that the bone marrow interstitial fluid is clearly distinct from peripheral blood plasma, both during active pediatric acute lymphoblastic leukemia and following induction chemotherapy. Either compartment was shaped differently by active leukemia, with the bone marrow interstitial fluid being rich in extracellular vesicle components and showing protease dysregulation while the peripheral blood plasma showed elevation of immune regulatory proteins. Following chemotherapy, the BMIF showed signs of cellular remodeling and impaired innate immune activation while the peripheral blood plasma was characterized by restored lipid homeostasis.

Conclusion
This study provides a comprehensive examination of the fluid portion of the acute lymphoblastic leukemia microenvironment and finds the contribution of either microenvironment to tumourigenesis.
Effect of an online resourcefulness training in improving psychological well-being of front-line medical staff: a quasi-experimental study
Authors: Zhang Dandan , Jia Yitong , Chen Yongjun , Meng Ge , Zhuang Xinqi , Chen Li , Wang Dongmei , Zhang Yin-Ping 
Published: September 2022

Abstract
Background
The global COVID-19 pandemic is still not under effective control, and strong workplace supports with comprehensive mental health interventions are urgently needed to help medical staff effectively respond to the pandemic. This study aimed to verify the effect of an online resourcefulness training program on the resourcefulness, and psychological variables of front-line medical staff working in the COVID-19 isolation ward.

Design
A pre-test and post-test quasi-experimental design with control group was employed.

Participants
A total of 60 participants working in two isolation wards were recruited via convenience sampling. The two isolation wards were randomly assigned to the control group (isolation ward 1, n = 30) and the intervention group (isolation ward 2, n = 30).

Intervention
The participants were trained online by video conferences and WeChat. The control group received conventional training (e.g., psychological training, psychological counseling), while the intervention group received a 4-h online resourcefulness training. Both groups learned updated guidelines of COVID-19 simultaneously via video conference. The primary outcomes (resourcefulness, anxiety, depression and coping styles) and the secondary outcome (psychological resilience) were measured before intervention and three time points after intervention.

Results
After the intervention and one week after the intervention, the resourcefulness, resilience, and positive response scores of the participants in the intervention group were significantly higher than those of the control group. The anxiety and negative response scores in the intervention group were significantly lower than those of the control group (all p < 0.05).
One month after the intervention, the scores of resourcefulness, tenacity, and positive response of the intervention group were higher than those of the control group (all p < 0.05). Repeated measures analysis of variance showed that the two groups of participants had statistically significant changes in the time-based effect and group-based effect in resourcefulness, resilience, anxiety scores and coping styles (p < 0.01).

Conclusion
The results showed that our online resourcefulness training can significantly improve the resourcefulness, resilience, and positive response scores and effectively reduce anxiety and depression scores of front-line medical staff. This demonstrates that online resourcefulness training would be an effective tool for the psychological adjustment of front-line medical staff in fighting against COVID-19.
Validity of score interpretations on an online English placement writing test
Authors: Choi Yun Deok 
Published: September 2022

AbstractA much-debated question in the L2 assessment field is if computer familiarity should be considered a potential source of construct-irrelevant variance in computer-based writing (CBW) tests. This study aims to make a partial validity argument for an online source-based writing test (OSWT) designed for English placement testing (EPT), focusing on the explanation inference. Score interpretations on the OWST are proposed and supporting evidence is sought in terms of test-takers’ self-confidence in and preferences for CBW tests (two interrelated aspects of computer familiarity) and L2 writing ability. Ninety-seven ESL students demonstrating two different levels (higher and lower levels) of L2 writing ability studying at a US university completed the OSWT and an online questionnaire asking about their attitudes towards CBW tests. A series of statistical and thematic analyses revealed that most of the test-takers held self-confidence in and preferences for CBW tests for reasons related to previous CBW experience (e.g., familiarity with CBW, useful tools/functions available on computers) regardless of L2 writing ability. The higher-level test-takers obtained significantly higher scores on the OSWT than their lower-level counterparts. Test-takers’ preferences were a significant predictor of the OSWT scores only in the higher-level group. The findings largely support the validity of proposed score interpretations on the OSWT. Implications are discussed in terms of test fairness and the construct of CBW tests.
Identifying moderating factors during the preschool period in the development of borderline personality disorder: a prospective longitudinal analysis
Authors: Boone Kiran , Vogel Alecia C. , Tillman Rebecca , Wright Amanda J. , Barch Deanna M. , Luby Joan L. , Whalen Diana J. 
Published: September 2022

Abstract
Background
Despite a growing literature detailing early childhood risk factors for borderline personality disorder (BPD), few studies have examined moderating factors that might mitigate or exacerbate the effects of those risk factors. The current study examined whether three preschool-age characteristics—impulsivity, emotional lability, and initiative-taking—moderated the relationship between known preschool-age risk factors and adolescent BPD symptoms.

Methods
We performed multilevel modeling analyses in a sample (n = 151) from the Preschool Depression Study, a prospective longitudinal study with assessments from preschool through adolescence. Preschool risk factors included adverse childhood experiences, internalizing symptoms, and externalizing symptoms measured with parent clinical interviews. Preschool moderating factors were assessed via parent report and observational coding of temperament and behavior. The Borderline Personality Features Scale for Children measured BPD symptoms in adolescence.

Results
We found that observed initiative-taking moderated the relationship between preschool internalizing symptoms and adolescent BPD symptoms (b = 0.57, p = .011) and moderated the relationship between preschool externalizing symptoms and adolescent BPD symptoms (b = 1.42, p = .013). Greater initiative-taking was associated with lower BPD risk for children with high internalizing or externalizing symptoms. Conversely, for children with low internalizing or externalizing symptoms, greater initiative-taking was associated with increased BPD risk.

Conclusions
We identify a potential moderating factor in BPD development, offer novel targets for screening and intervention, and provide a framework for using early childhood observational assessments in BPD research. Our findings suggest the need for future research on early moderating factors in BPD development, which could inform early childhood interventions targeting those factors to mitigate the effects of potentially less malleable risk factors.
Iron-gall inks: a review of their degradation mechanisms and conservation treatments
Authors: Melo Maria João , Otero Vanessa , Nabais Paula , Teixeira Natércia , Pina Fernando , Casanova Conceição , Fragoso Sara , Sequeira Sílvia O. 
Published: September 2022

Abstract
Iron-gall inks are an essential element of our written cultural heritage that is at risk of a total loss due to degradation. This degradation leads to the loss of the support, particularly the cellulose-based support. Intending to stabilize it, we have come a long way from the nineteenth-century cellulose nitrate laminations to the relatively recent phytate treatments; nevertheless, less invasive treatments are needed. To pave the way for developing safer and more sustainable treatments, tailored as much as possible to the object, this paper reviews the conservation treatments and the advances that have taken place over the last decade in our understanding of the degradation mechanisms of iron-gall inks, based on a careful selection of references to support a concise microreview. This discussion is based on the currently accepted models based on the Fe3+-gallate and the identification of degradation products for iron-gall inks observed in heritage objects, including manuscripts dating from the fourteenth to seventeenth centuries and drawings from the fifteenth to nineteenth centuries. The degradation promoted by iron-gall inks induces scission of cellulose through acid catalysis and/or redox reactions. The causes of these acid-base and redox reactions are also assessed. Finally, we detail the state-of-the-art conservation treatments used to mitigate iron gall ink deterioration, covering treatments from the late nineteenth century to the beginning of the twentieth century, followed by the presentation of current phytate treatments and new postphytate treatments.
Historical overview and current practice of peritoneal dialysis in Japan
Authors: Kawanishi Hideki 
Published: September 2022

AbstractIn the early days of peritoneal dialysis (PD) therapy, its limited duration and peritoneal deterioration were argued to be its disadvantages. Biocompatible solutions and hybrid therapy have been used in Japan to overcome these issues, which resulted in a decrease in encapsulating peritoneal sclerosis (EPS) incidence and an extension of PD continuation; these results have been disseminated worldwide. Peritoneal dialysis outcomes and practice patterns study (PDOPPS), a prospective observational study, has begun to confirm the outcomes of PD therapy, and sufficient evidence has been published, which has influenced the preparation of PD guidelines. Current thinking about PD emphasizes the need to maintain quality of life and life goals as care goals for patients and to provide high-quality care. However, we must conduct basic research on the prevention of peritoneal deterioration.