bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calibration Techniques Encompassing Survey Sampling, Missing Data Analysis and Causal Inference
Authors: Zhang Shixiao , Han Peisong , Wu Changbao 
Published: August 2022

SummaryWe provide a critical review on calibration methods developed in three different areas: survey sampling, missing data analysis and causal inference. We highlight the connections and variations of calibration techniques used in missing data analysis and causal inference to conventional calibration weighting and estimation in survey sampling and provide a common framework through model‐calibration and empirical likelihood to unify different calibration methods proposed in recent literature. The goal is to demonstrate the success and effectiveness of calibration methods in achieving some highly desired properties for missing data analysis and causal inference.
The burden of costs on health services by patients with neuro‐ischaemic ulcers in Singapore
Authors: Nazeha Nuraini , Meng Lingyan , Ho Pei , Ng Yi Zhen , Graves Nicholas 
Published: August 2022

AbstractThe economic burden of neuro‐ischaemic ulcers (NIU) is expected to increase because of rising prevalence of comorbidities in an aging population. We aim to estimate healthcare resources consumed by NIU patients, and to quantify the extent to which factors explain variation in cost‐related outcomes. We analysed retrospective patient‐level cohort data for NIU patients from a tertiary hospital registry in Singapore, from 2013 to 2017, using generalised linear regression models. The outcome variables were the length of stay per admission; inpatient and outpatient bill per admission; and, if they had an Emergency Department visit. Cost outcomes were reported in Singapore dollars (S$). A total of 1682 patients were included, and the mean age was 69.9 years (±13.0). An average patient incurred a length of stay of 38.7 days, 7.9 inpatient dressing sessions, an inpatient bill of S$33 096, 11.3 outpatient dressing sessions, and an outpatient bill of S$8780. Inpatient services per patient cost 73.5% higher than outpatient services. NIU patients with multiple (>3) comorbid conditions, peripheral artery disease, or chronic kidney disease incurred longer hospitalisation and higher inpatient bill. Patients with diabetes mellitus and coronary artery disease had higher odds of incurring an ED visit. Patients with coronary artery disease, hyperlipidaemia, kidney complications, or obesity incurred higher outpatient bills. NIU treatment imposes a significant economic burden, especially with inpatient services.
Virulence and biofilm inhibition of 3-methoxycinnamic acid against Agrobacterium tumefaciens
Authors: Pan Deng , Wang Xing-Yun , Zhou Jin-Wei , Yang Liang , Khan Abbas , Wei Dong-Qing , Li Jun-Jian , Jia Ai-Qun 
Published: August 2022

Abstract

Aims
In the current study the anti-virulence and anti-biofilm activities of the cinnamic acid derivative, 3-methoxycinnamic acid, was investigated against Agrobacterium tumefaciens.

Methods and Results
Based on the disc diffusion test and β-galactosidase activity assay, 3-methoxycinnamic acid was shown to interfere with the quorum sensing (QS) system of A. tumefaciens. Crystal violet staining assay, phenol-sulfuric acid method, Bradford protein assay and confocal laser scanning microscopy (CLSM) revealed that the biofilm formation of A. tumefaciens was inhibited after the treatment of 3-methoxycinnamic acid. Employing high-performance liquid chromatography (HPLC) analysis of culture supernatant revealed that the production of 3-oxo-octanoylhomoserine lactone (3-oxo-C8-HSL) decreased concentration-dependently after treatment with 3-methoxycinnamic acid. Swimming and chemotaxis assays also indicated that 3-methoxycinnamic acid had a good effect on reducing the motility and chemotaxis of A. tumefaciens. In addition, the RT-qPCR, molecular docking and simulations further demonstrated that 3-methoxycinnamic acid could competitively inhibit the binding of 3-oxo-C8-HSL to TraR and down-regulate virulence-related genes.

Conclusions
3-Methoxycinnamic acid is proved to have good anti-virulence and anti-biofilm activities against A. tumefaciens.

Significance and Impact of the Study
This is the first study that investigates the anti-virulence and anti-biofilm activities of 3-methoxycinnamic acid against A. tumefaciens. With its potential QS-related virulence and biofilm inhibitory activities, 3-methoxycinnamic acid is expected to be developed as a potent pesticide or adjuvant for the prevention and treatment of crown gall caused by A. tumefaciens.
Editorial: Environmental conditions for growing healthy children
Authors: Luby Joan L. 
Published: August 2022

The deleterious developmental effects of exposure to early adversity have been well documented in the scientific literature. The finding that poverty in early childhood is among the most robust predictors of a range of poor developmental outcomes has been well known for decades. More recently, evidence that early experiences of poverty and related forms of adversity negatively impact the function and structure of the developing brain have also emerged. Retrospective studies linking poor physical health outcomes to adverse childhood experiences (ACES) highlighted the more global nature of these risk factors to wellbeing and follow‐up prospective studies have since confirmed these findings (Brown et al., 2009). Alternatively, it has become increasingly clear that early experiences of stimulation, nurturance, and caregiver support promote positive development outcomes with emerging evidence for tangible impacts on neurodevelopment in humans (Luby et al., 2021). However, the scientific and public health community has yet to synthesize these related bodies of data and develop a plan of action related to their over‐reaching and global importance to protecting and promoting childhood health and development more generally despite numerous calls to do just that (Farah, 2018; Luby et al., 2020).
Ecology and the evolution of sex chromosomes
Authors: Meisel Richard P. 
Published: August 2022

AbstractSex chromosomes are common features of animal genomes, often carrying a sex determination gene responsible for initiating the development of sexually dimorphic traits. The specific chromosome that serves as the sex chromosome differs across taxa as a result of fusions between sex chromosomes and autosomes, along with sex chromosome turnover—autosomes becoming sex chromosomes and sex chromosomes ‘reverting’ back to autosomes. In addition, the types of genes on sex chromosomes frequently differ from the autosomes, and genes on sex chromosomes often evolve faster than autosomal genes. Sex‐specific selection pressures, such as sexual antagonism and sexual selection, are hypothesized to be responsible for sex chromosome turnovers, the unique gene content of sex chromosomes and the accelerated evolutionary rates of genes on sex chromosomes. Sex‐specific selection has pronounced effects on sex chromosomes because their sex‐biased inheritance can tilt the balance of selection in favour of one sex. Despite the general consensus that sex‐specific selection affects sex chromosome evolution, most population genetic models are agnostic as to the specific sources of these sex‐specific selection pressures, and many of the details about the effects of sex‐specific selection remain unresolved. Here, I review the evidence that ecological factors, including variable selection across heterogeneous environments and conflicts between sexual and natural selection, can be important determinants of sex‐specific selection pressures that shape sex chromosome evolution. I also explain how studying the ecology of sex chromosome evolution can help us understand important and unresolved aspects of both sex chromosome evolution and sex‐specific selection.
A new set of microsatellite markers for Phoxinus lumaireul senso lato, Phoxinus marsilii and Phoxinus krkae for population and molecular taxonomic studies
Authors: Vucić Matej , Jelić Mišel , Klobučar Göran , Jelić Dušan , Gan Han Ming , Austin Chris , Guyonnet Daniel , Giraud Isabelle , Becking Thomas , Grandjean Frederic 
Published: August 2022

AbstractMinnows of the genus Phoxinus are common and an often highly abundant fish species in Palearctic freshwater habitats. Phoxinus species have a complex evolutionary history, phylogenetic relationships are not well understood and there are a number of unresolved taxonomic problems. There are currently 23 different mitochondrial genetic lineages identified in the genus Phoxinus, 13 of which are recognized as valid species. The taxonomic status of these lineages requires resolution, including the degree to which they can interbreed. Suitable nuclear molecular markers for studies of population divergence and interbreeding between morphotypes and mitochondrial lineages are lacking for Phoxinus species. Therefore, the authors developed a set of microsatellite markers using genomic information from Phoxinus lumaireul and tested their suitability for this and two related species, Phoxinus krkae and Phoxinus marsilii. Out of 16 microsatellite candidate loci isolated, 12 were found to be in Hardy–Weinberg equilibrium when tested on two P. lumaireul senso lato populations. Seven loci amplified across the three species, enabling the study of intraspecific genetic diversity and population structure within P. marsilii and P. krkae. The markers were able to clearly resolve differences among the three tested species, including the recently described P. krkae, and are therefore suitable for the detection of introgression and hybridization among populations consisting of mixtures of two or more of P. lumaireul s. l., P. marsilii and P. krkae.
Examining the relationship between perinatal anxiety, COVID‐19 phobia and birth type preferences: A cross‐sectional study
Authors: Akgün Mehtap , Turgut Yasemin , Güdül Öz Hatice , Yangin Hatice , Boz İlkay 
Published: August 2022

AbstractAimTo examine the relationship between the COVID‐19 phobia and perinatal anxiety levels and birth type preferences of pregnant women in the third trimester.DesignThis was designed a cross‐sectional study using the non‐random convenience sampling method following the STROBE checklist.MethodsThe research was conducted with 315 pregnant women from April to May 2021. Data were collected using a personal information form, the COVID‐19 Phobia Scale, and the Perinatal Anxiety Screening Scale (PASS).ResultsWe found a positive and moderate correlation between the total scores for perinatal anxiety and COVID‐19 phobia. During the COVID‐19 pandemic, 4.1% of the participants changed their birth type preferences and this change was statistically significant according to McNemar's test. During the COVID‐19 pandemic, the women changed their birth type preferences in favour of caesarean section. However, there was no statistically significant difference between those who changed their birth type preferences and those who did not in terms of perinatal anxiety or COVID‐19 phobia levels. Women with no access to prenatal follow‐up visits due to the COVID‐19 pandemic had higher mean PASS scores and higher mean scores for the perfectionism, control and trauma subscale. The scores for general worry and specific fears were lower among individuals who had obtained information about birth types in prenatal follow‐up visits. Also, perinatal anxiety and COVID‐19 phobia levels were higher among pregnant women who were worried about giving birth in hospital compared to those who were not worried.ConclusionWe conclude that COVID‐19 phobia has increased women's perinatal anxiety, causing them to change their birth type preferences in favour of caesarean section.Relevance to clinical practiceWe recommended that healthcare professionals take COVID‐19 phobia and perinatal anxiety into account when counselling pregnant women about birth types to improve prenatal care.No patient or public contributionNo patient or public contribution was required to design, to outcome measures or undertake this research. Patients/members of the public contributed only to the data collection. Data were obtained from pregnant women in the third trimester, who came to a regional hospital‐affiliated obstetrics polyclinic for routine prenatal follow‐up visits.
Economics of drug‐coated balloons for arteriovenous fistula stenosis in Japan and Korea based on the IN.PACT AV access trial
Authors: Chun Ho Jong , Cao Khoa N. , Haruguchi Hiroaki , Choi Hyunsook , Yoshikawa Mayuko , Holden Andrew , Pietzsch Jan B. 
Published: August 2022

AbstractAimThe recent IN.PACT AV Access study found drug‐coated balloon therapy to be associated with reduced reinterventions compared to percutaneous transluminal angioplasty using standard balloons in the management of arteriovenous fistula stenosis. The economic implications of drug‐coated balloon use in Asia, including Japan and Korea, remain unknown.MethodsA decision‐analytic model was developed to calculate strategy‐specific costs for Korea and Japan through 5‐year follow‐up. The analysis assumed maintained therapy benefit beyond current trial follow‐up of 1 year in the base case, with several alternative scenarios explored in sensitivity analysis. Costs were derived from claims and reimbursement data, and projections were evaluated at 3 and 5 years post‐index procedure.ResultsModel‐projected access circuit reintervention events for drug‐coated versus standard balloons were 1.70 versus 2.76 (−1.06) and 2.53 versus 4.10 (−1.57) at 3 and 5 years in the base case. Corresponding 3‐ and 5‐year costs were ₩6 211 103 versus ₩7 605 553 (−₩1 394 451) and ₩7 766 051 versus ₩10 124 954 (−₩2 358 904) in Korea, and ¥1 469 824 versus ¥1 504 161 (−¥34 337) and ¥1 956 931 versus ¥2 106 632 (−¥149 701) in Japan. In scenario analyses, drug‐coated balloons remained cost saving at 3‐ and 5‐year follow‐up in Korea, but required up to 5 years to reach cost‐savings in Japan. Drug‐coated balloon use in reinterventions increased projected savings, as did younger treatment age.ConclusionTreatment of arteriovenous fistulas with the IN.PACT AV drug‐coated balloon, based on preliminary data, may lead to meaningful reductions in reintervention costs that would render it cost‐saving at timeframes of around 1 year in Korea and between 3 and 5 years in Japan.
Light, chromatin, action: nuclear events regulating light signaling in Arabidopsis
Authors: Patitaki Eirini , Schivre Geoffrey , Zioutopoulou Anna , Perrella Giorgio , Bourbousse Clara , Barneche Fredy , Kaiserli Eirini 
Published: August 2022

SummaryThe plant nucleus provides a major hub for environmental signal integration at the chromatin level. Multiple light signaling pathways operate and exchange information by regulating a large repertoire of gene targets that shape plant responses to a changing environment. In addition to the established role of transcription factors in triggering photoregulated changes in gene expression, there are eminent reports on the significance of chromatin regulators and nuclear scaffold dynamics in promoting light‐induced plant responses. Here, we report and discuss recent advances in chromatin‐regulatory mechanisms modulating plant architecture and development in response to light, including the molecular and physiological roles of key modifications such as DNA, RNA and histone methylation, and/or acetylation. The significance of the formation of biomolecular condensates of key light signaling components is discussed and potential applications to agricultural practices overviewed.
Single‐cell transcriptomics dissects premalignant progression in proliferative verrucous leukoplakia
Authors: Zhong Liang , Wang Fei , Liu Dan , Kuang Wenjing , Ji Ning , Li Jing , Zeng Xin , Li Taiwen , Dan Hongxia , Chen Qianming 
Published: August 2022

AbstractObjectiveProliferative verrucous leukoplakia (PVL) is characterized by a spectrum of clinicopathological features and a high risk of malignant transformation. In this study, we aimed to delineate the dynamic changes in molecular signature during PVL progression and identify the potential cell subtypes that play a key role in the premalignant evolution of PVL.MethodsWe performed single‐cell RNA sequencing on three biopsy samples from a large PVL lesion. These samples exhibited a histopathological continuum of PVL progression.ResultsBy analyzing the transcriptome profiles of 27,611 cells from these samples, we identified ten major cell lineages and revealed that cellular remodeling occurred during the progression of PVL lesions, including epithelial, stromal, and immune cells. Epithelial cells are shifted to tumorigenic states and secretory patterns at the premalignant stage. Immune cells showed growing immunosuppressive phenotypes during PVL progression. Remarkably, two novel cell subtypes INSR+ endothelial cells and ASPN+ fibroblasts, were discovered and may play vital roles in microenvironment remodeling, such as angiogenesis and stromal fibrosis, which are closely involved in malignant transformation.ConclusionOur work is the first to depict the cellular landscape of PVL and speculate that disease progression may be driven by functional remodeling of multiple cell subtypes.
ERP responses to sexual cues among young women attracted to men
Authors: Huberman Jackie S. , Mangardich Haykaz , Sabbagh Mark A. , Chivers Meredith L. 
Published: August 2022

AbstractPrevious event‐related potential (ERP) studies reported larger N170, P3, and late positive potential (LPP) amplitudes to sexual than nonsexual stimuli. These ERPs may not be specifically sensitive to processing sexual cues, however, because the sexual stimuli included information beyond sexual cues (e.g., faces, bodies, social interaction) to a greater extent than comparison stimuli. We investigated ERPs to stimuli that focused on sexual and nonsexual body regions, in different states of readiness for activity, to elucidate neural responses involved in processing sexual cues. Forty cisgender, primarily white, undergraduate women who were attracted to men (Mage = 18.6, SD = 0.9) viewed images that varied by male body part (penis, arm) and activity state (rest, poised for activity). Participants viewed 40 images per category (flaccid penises, erect penises, outstretched arms, bent arms). Electroencephalography (EEG) was recorded using a 128‐channel net, time‐locked to the onset of each image. Using a whole‐head cluster‐mass approach, we found that the P3 was sensitive to sexual readiness—P3 amplitudes were larger to erect than flaccid penises, but not to bent than outstretched arms. The N170 and LPP components did not show evidence of similarly specific responses to sexual readiness, revealing potential dissociation of different neural processes commonly elicited in response to more complex sexual stimuli. An additional novel finding was that an anterior N270‐400 was sensitive to sexual readiness. Findings clarify the brain's rapid responses to sexual stimuli, setting the stage for future research aimed at better understanding the neurocognitive processes that contribute to the coordination of sexual arousal.
Abortion, mental health and epistemologies of psychological knowledge and ignorance
Authors: Hooberman Laura , Ozoguz Sedef 
Published: August 2022

AbstractThis article traces the origins and production of the Post Abortion Syndrome (PAS), a fallacious bio‐ideological construct which alleges a link between abortion and mental health risks. Through the work of social actors (anti‐abortion activists, policy makers, funders, advocates, and researchers) involved in its construction, this diagnosis was disseminated and diffused into public policy in the US and is used as ‘evidence’ in deterring people from having abortions. This is despite overwhelming evidence that abortion poses no greater mental health risks than carrying an unintended pregnancy to term. Circulation of this fallacious discourse invalidates, distorts, and homogenizes the lived experiences of reproductive people, contributing to epistemologies of ignorance as pertains to women's health and reproductive experiences. This article examines the invention of PAS, exploring its political underpinnings and ongoing effects. We unearth how psychology and medicine have been coopted to function as handmaidens in passing conservative legislation, attending to the process of willful distortion underpinning this history. Uncovering the systemic nature of the production of ignorance around reproductive people's bodies and psychologies is a necessary step in providing liberatory epistemologies for pregnant people and in enabling individuals to make their own meaning of their abortions.
Hypoxia‐targeting therapy for intestinal T‐cell lymphoma in dogs: Preclinical study using 3D in vitro models
Authors: Yamazaki Hiroki , Tanaka Toshiyuki , Nishida Hidetaka , Hatoya Shingo , Akiyoshi Hideo 
Published: August 2022

AbstractThe transcription factor hypoxia‐inducible factor 1α (HIF‐1α) is activated in response to oxygen deficiency, and is expressed in several cancers under intratumoral hypoxic stress that arises during pathogenic processes. Hypoxic stimulation enhanced the growth potential of canine lymphoma cells by activating the HIF‐1α signalling pathway in a previously reported study. The aim of this study was to establish a molecular design strategy for a novel hypoxia‐targeting therapy for intestinal T‐cell lymphoma (ITL) in dogs. We assessed the relationship between immunohistochemistry‐based HIF‐1α expression and clinical information, including signalment, tumour area, clinical signs, systemic diseases, treatment protocol, follow‐up information, chemotherapy response and overall survivals (OS), using 48 tissue samples from dogs with ITL. We investigated the effects of hypoxic stimulation on the biological behaviour of cell lines from three different types of canine ITL. We assessed the effects of evofosfamide (Evo; hypoxia‐activated prodrug) on cell lines cultured under hypoxic conditions. Our data showed that treatment response and overall survival might be significantly decreased in dogs with higher HIF‐1α expression than in those with lower HIF‐1α expression. Hypoxic culture (1% O2, 72 h) enhanced the invasiveness of cell lines and decreased their sensitivity to CCNU, resulting in hypoxia‐dependent aggressive behaviour. Sensitivity to Evo significantly increased in cell lines cultured under hypoxia compared with those cultured under normoxia, which exhibited hypoxia‐dependent apoptosis. Additionally, Evo downregulated HIF‐1α expression in cell lines cultured under hypoxia, suggesting that Evo might inhibit cell growth by inactivating HIF‐1α‐dependent cell signalling. Our results revealed the preclinical antitumor activity of Evo and provide a rationale for treatment strategies for dogs with ITL.
Macroscopic Architecture Design of Lithium Metal Electrodes: Impacts of Millimeter-Size Hollows on Economization, Cyclability, and Utilization
Authors: Li Xiangjie , Zhao Yanfei , Song Yicheng , Zhang Junqian , Lu Bo 
Published: August 2022

Abstract
A macroscopic architecture design of lithium metal electrodes for solving the problem of extremely excessive lithium metal is proposed in this paper. By employing a simple mechanical processing method, macroscopic hollows within lithium foils are introduced, and consequently, the amount of lithium metal has economized significantly. The cyclability of lithium foils with millimeter-size hollows is evaluated jointly via modeling and experiments. The results suggest that the well-designed macroscopic hollow causes controllable sacrifices of battery cycling performances and considerably boosts the utilization of lithium metal. The relationship of economization, cyclability, and utilization of lithium metal is also discussed. The universality of the results is also verified in different battery systems. Meanwhile, the initial hollows are found to heal morphologically after a series of electrochemical cycles, and the existence of lithium metal in the healing product is also confirmed, indicating that hollows provide room for the in-plane lithium dendrite growth. Based on these findings, this work provides a new perspective on the architectural design of lithium metal electrodes.