bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intelligent Lifecycle Management of Landscapes through Building Information Modeling and Mixed Reality
Authors: Zhao Xuefeng , Li Mengxuan , Sun Zhe , Zhao Yue , Li Danyang , Gai Yihao , Wang Jingjing , Huang Chun , Yu Lei , Wang Sicong , Zhang Meng , Huang Lingli 
Published: May 2022

Abstract
Context
Intelligent lifecycle management is essential to ensure the sustainable development of landscapes. Unfortunately, various challenges still exist in achieving model creation, data transmission, project coordination, and plant maintenance.
Objectives
Integrated use of Building Information Modeling (BIM) and Mix Reality (MR) techniques could help establish digital representations of objects with semantic information. Such integration of BIM and MR offers instinctual interactions by blending the physical and digital worlds.
Methods
The authors propose a framework based on the BIM and MR for intelligent management during the planting and maintenance processes of landscapes. The framework includes two parts, 1) to establish a parameterized method for detailed plant model creation based on CAD, Excel, Dynamo, and Revit, and 2) to establish a collaborative management method for plant maintenance through BIM model and MR device.
Results
The intelligent lifecycle management framework proposed in this study can achieve 1) establish detailed models for various plants, 2) capture spatiotemporal changes of plants across four seasons for model updating, 3) allow real-time data transmission for effective project coordination, and 4) remote maintenance of various plants. Furthermore. The authors use the Jinhu Park project in the Xiong'an New District of China as a case study to validate the proposed framework.
Conclusions
Results show that the integrated use of BIM and MR for lifecycle management of landscapes could help to improve the level of collaboration among multiple participants and the quality of plant lifecycle management. The proposed framework could support the sustainable development of landscapes in real practice.
Metabolomics Analysis of the Aqueous Humour Uncovers Potential Metabolite Biomarkers for Early Differential Detection of Macular Edema
Authors: Jiang Dan , Yan Congcong , Ge Lina , Yang Chun , Huang Ying , Chen Chonghua , Chen Wei , Zhou Meng , Lin Bing 
Published: May 2022

Abstract
Background: Macular edema (ME) is a major complication of several retinal diseases, and multiple mechanisms are involved in its development. This study aims to investigate the metabolite profiles of the aqueous humour in patients with ME of different aetiologies and identify potential metabolite biomarkers allowing ME diagnosis in the very early stages of the disease.Results: The metabolic profiles of aqueous humour differed significantly between ME patients and healthy controls, and 105 differentially expressed metabolites were identified. Pathway analysis showed that the differentially expressed metabolites are mainly involved in lipid metabolism and amino acid metabolism. Further comparison analysis also revealed significant differences in the metabolic composition of aqueous humour of ME patients derived from age-related macular degeneration (AMD), diabetic retinopathy (DR) and branch retinal vein occlusion (BRVO). In total, 39 and 79 aetiology-specific altered metabolites were identified for AMD-ME and DME, respectively. Finally, a novel aqueous humour-derived machine learning-based metabolic model was developed, which successfully validated in the test cohort with an area under the receiver operating characteristic curve of 0.79 for AMD-ME, 0.94 for DME and 0.77 for BRVO-ME.Conclusions: Our study illustrates the underlying metabolic basis of aqueous humour for different aetiologies across ME populations and identifies aqueous humour-derived metabolite biomarkers that will improve differential diagnosis and treatment stratification for ME of different aetiologies.
A sanitary safe method for screening mosquito repellents on Anopheles pseudopunctipennis and Aedes aegypti
Authors: Costa Agustín Alvarez , Gonzalez Paula Valeria , Harburguer Laura Vanesa , Masuh Hector Mario 
Published: May 2022

Abstract
One of the main vectors for malaria in Latin-America is Anopheles pseudopunctipennis (Theobald), whereas Aedes aegypti (L.) is the primary vector of dengue, yellow fever, Zika, and chikungunya viruses. The use of repellents is recommended as personal protection method against these vectors (or mosquitoes), however there are very few studies evaluating the effect of repellents on An. pseudopunctipennis. The use of a Petri dish to study repellence has been applied by several authors on flies, cockroaches, kissing bugs and mosquitoes, being a valuable technique for species that are difficult to breed under laboratory conditions, such as An. pseudopunctipennis, which could be infected by parasites. In the present study we evaluated the repellence of the essential oil of E. nitens, its main component (1,8-cineole) and the commercial repellent DEET on An. pseudopunctipennis and Ae. aegypti females using the plaque repellency method coupled to EthoVision XT10.1 video-tracking software. Repellent effect and locomotor activity were studied through a repellence index (RI) together with an axis constructed from the behavioral variables obtained using the tracking software. DEET repellent effect was observed at 0.01mg/mL for Ae. aegypti and 0.01 and 0.1mg/mL for An. pseudopunctipennis. In addition, the essential oil showed significant repellence at 1 and 10 mg/mL for Ae. aegypti, and 1, 5, 10 and 25 mg/mL for An. pseudopunctipennis, no significant response was detected to 1,8-cineole at any concentration for neither of the two species. This is the first study that evaluates these compounds on An. pseudopunctipennis females and quantify their effects on the activity of both species.
A novel domain duplication SlitFAR3 involved in sex pheromone biosynthesis in Spodoptera litura
Authors: Zhang Biyun , Li Fengqi , Qu Cheng , Fu Yuejun , Luo Chen 
Published: May 2022

Abstract
Fatty Acyl reductases (FARs) are key enzymes that participate in sex pheromone biosynthesis by reducing fatty acids to fatty alcohols. Lepidoptera typically harbor numerous FAR gene family members. While the FAR gene of moth is involved in the biosynthesis of sex pheromone, the key FAR gene of Spodoptera litura is still unclear. In this work, we predicted 30 FAR genes from S. litura genome. A special domain duplication was found with gene SlitFAR3, which exhibited high and preferential expression in the sexually mature female pheromone glands, and its expression pattern was rhythmic during the scotophase of sex pheromone production. Functional expression in yeast cells combined with comprehensive gas chromatography (GC) indicates that SlitFAR3 gene was able to catalyze four methyl ester precursors into corresponding fatty alcohol products. The domain duplication FAR genes were further found to exist in 11 species of the other 19 Lepidoptera insect. The phylogenetic tree showed SlitFAR3 was grouped with the other six FAR with domain duplication under the pgFAR subfamily clade, which is important candidate FAR genes participating in sex pheromone biosynthesis, and the other eight FAR domain duplication genes of 7 species were scattered in different clades. Domain duplications may facilitate the evolutionary diversification of protein sequences, which played diverse roles. This study is the first to focus on the special phenomenon of FAR domain duplication, which will advance understanding the biosynthesis-related genes from the perspective of evolutionary biology.
Variation in Phenotypic and Genotypic Characteristics of Yam Zygotic Seeds Raised in Drip System Hydroponics
Authors: OSSAI Chukwunalu Okoile , Shonde Temitope E. , Edemodu Alexandar , Udofia Adetutu F. , Olusola Theresa , Balogun Morufat O. 
Published: May 2022

Abstract
Yam is traditionally propagated using the tuber part (seed yam) as the use of yam seed is constrained by the non-synchronization of flowering and the rare occurrence of zygotic seed production will generate segregation in the offsprings (not true-to-type) theoretically. However, there is dearth of information on the validation of this theory in practical terms. This study, thus evaluates individual zygotic seeds of 2 improved open pollinated improved varieties (TDr 98/00917 and TDr 95/00893 x TDr 95/00903) and one landrace (TDr 3010). Ten zygotic seeds of the three accessions were planted in hydroponics trough containing buffered and moistened cocopeat substrate. The individual plants were phenotyped following standard operating procedures. DNAs were extracted from young fresh leaves at 6 weeks old and were used for Polymerase Chain Reaction (PCR) with 10 codominant SSR markers. The PCR bands were scored based on presence and absence. The phenotypic and genotypic scores were used to generate a dendogram and principal clusters. Result obtained revealed varied clustering arrangements among the individual plants, irrespectively of the accessions in both phenotypic and genotypic dendograms. This reveals the differences that exists between individual plants of same accession.
Transcription factor myc2-like, binding to ABRE element of OsCYP2 promoter, enhance salt tolerance in Oryza sativa
Authors: Liu Hongbo , Cui Peng , Zhang Bingxin , Zhu Jinbo , Liu Cui , Li Qingyang 
Published: May 2022

Abstract
Cyclophilins, a type of peptidyl-prolyl cis-trans isomerase, function as an important molecular chaperone in series of biological processes. However, the expression pattern and signal transduction pathway of cyclophilins is still unclear. Hereby, it was clarified that the promoter of OsCYP2 is a constitutive expression pattern which by GUS staining in transformants with pOsCYP2:GUS genetic transformation. Simultaneously, it is found that the sequence of promoter had not only core elements but also contained inducible elements. Then, the ABA responsive element was used to cDNA library screening, a transcription factor myc2-like was identified by yeast one hybrid and confirmed though electrophoretic mobility shift assay. Furthermore, the relative expression showed that myc2-like was induced by abscisic acid. In addition, myc2-like overexpression could enhance salt tolerance in transformants and partial restore cyp2-RNAi line. Together, we explored a novel transcriptional signal mediated by myc2-like, a potential regulator of salt stress physiological processes in rice.
Impact of increased UV curing time on the curing depth of photosensitive resins for 3D Printing
Authors: Guttridge Callum , Shannon Alice , O’Sullivan Aidan , O’Sullivan Kevin J. , O’Sullivan Leonard W. 
Published: May 2022

Abstract
Background
3D printing (3DP) photosensitive resins are commonly used to produce patient-specific solutions in the fields of medicine and dentistry. These resins are toxic in their liquid state. To ensure that all resin has solidified and parts are safe for use, post-processing must be carried out after printing. Parts are first washed in IPA, allowed to dry, and then post-cured under ultra-violet light (UV), and sometimes heat. As 3DP is commonly utilised for its ability to create custom and rapid solutions, it is expected that a different geometry will be produced almost every time. Currently, post-processing guidance is supplied specific to a material, with the caveat that post-curing times should be extended for larger or complex parts. The aim of this study was to assess the effect of extending post-curing times for photosensitive resin printed parts.
Method
Two commercially available vat-polymerisation 3DP systems were used to print hollow 60mm diameter spheres. Two opaque white, two opaque black, and two translucent amber resins were used. The spheres were filled with liquid resin, then UV post-cured at intervals of 100, 200, 300, 400 and 500% of the recommended guidance. The spheres were sectioned along the centreline and radial measurements taken of the cured depth.
Results
The results showed that both translucent amber materials cured to full depth at the 100% interval, whereas none of the white or black opaque materials cured to full depth, even at 500% of the recommended guidance.
Conclusions
This suggests opacity has a considerable effect on the depth of cure in photosensitive resins, and that the use of opaque resins increases the possibility of uncured resin remaining inside parts.
Disaster preparedness knowledge and its relationship with triage decision-making among hospital and pre-hospital emergency nurses- Ardabil, Iran
Authors: Azizpour Islam , Mehri Saeid , Soola Aghil Habibi 
Published: May 2022

Abstract
Objective: Hospital and pre-hospital emergency nurses are at the forefront of disaster response. This study was conducted to determine the level of "disaster preparedness knowledge" and its relationship with "triage decision-making" among hospital and pre-hospital emergency nurses in Ardabil province.Methods: A descriptive cross-sectional study was performed on 472 hospital and pre-hospital emergency nurses in Ardabil Province in the northwest of Iran, using a convenience sampling method. Data were collected using valid and reliable self-reported questionnaires, including the "Emergency Preparedness Information questionnaire" and "Triage Decision-making inventory". Results: Multiple linear regression analysis showed; triage decision-making, age, city of residence, training in disaster preparedness, Working on duty during a disaster, and training organization variables as predictors of disaster preparedness knowledge in hospital and pre-hospital emergency nurses (p<0.05).Conclusion: Hospital and pre-hospital emergency nurses who have higher disaster preparedness knowledge, have higher triage decision-making skills. It is suggested that the managers of educational and medical centres and professional organizations provide conditions for training and increasing disaster preparedness of Hospital and pre-hospital emergency nurses according to their age and place of residence.
Improving Biogas Production from Animal Manure By Batch Anaerobic Digestion
Authors: Mohammed Muetaz , Belkair Ahmad , Hassan Razena , Jirhiman Abraheem , Ahmeedah Ali 
Published: May 2022

Abstract
This study presents the improved biogas production by determining optimum pH and insulation reactor from cow, sheep, and poultry manure (which are the most available in our location). The experiment was carried out in 2000 mL digester put in bath water at 37 0C. The mixing ratio of animal manure and water used was 1:1 in 12 days of Hydraulic Retention Time (HRT). Produced gas was measured by the volumetric water replacement method. In the first phase, the best pH will be chosen in the experimental test with different pH ranges. Three set-ups were prepared with different pHs (6.5, 7, 7.5). The results showed that the pH had significant effects on biogas yield, where pH 7 had the highest biogas production and pH 6.5 is the lowest. In the second phase, investigated the effect of insulation of reactor on the biogas yield. The biogas was produced from an insulated reactor higher than the photoreactor because of the growth of algae which produces oxygen in the photoreactor. Therefore it is given that the study which gave a maximum yield of biogas from the batch digestion process might be met the future energy demand.
Combining a nanoparticle-mediated radioimmunotherapy with dual blockade of LAG3 and TIGIT improves the treatment efficacy in anti-PD1 resistant lung cancer
Authors: Hu Yun , Paris Sébastien , Bertolet Genevieve , Barsoumian Hampartsoum , He Kewen , Sezen Duygu , Chen Dawei , Wasley Mark , SILVA Jordan DA , Mitchell Joylise A , Voss Tiffany A , Masrorpour Fatemeh , Leyton Claudia Kettlun , Yang Liangpeng , Leuschner Carola , Puebla-Osorio Nahum , Gandhi Saumil , Nguyen Quynh-Nhu , Cortez Maria Angelica , Welsh James W. 
Published: May 2022

Abstract
Background: While improvements in radioimmunotherapy have made significant headway in improving outcomes for cancer patients, this treatment approach has nevertheless proven ineffective at controlling the majority of malignancies. One of the mechanisms of resistance to radioimmunotherapy is that immune cells may be suppressed via the myriad of different immune checkpoint receptors. Therefore, simultaneous blockade of multiple immune checkpoint receptors may enhance the treatment efficacy of radioimmunotherapy.Methods: We combined NBTXR3-enhanced localized radiation with the simultaneous blockade of three different checkpoint receptors: PD1, LAG3, and TIGIT and tested the treatment efficacy in an anti-PD1 resistant lung cancer model in mice. 129Sv/Ev mice were inoculated with fifty thousand αPD1-resistant 344SQR cells in the right leg on day 0 to establish primary tumors and with the same number of cells in the left leg on day 4 to establish the secondary tumors. NBTXR3 was intratumorally injected to the primary tumors on day 7, which were irradiated with 12 Gy on days 8, 9, and 10. Anti-PD1 (200 mg), αLAG3 (200 mg), and αTIGIT (200 mg) were given to mice by intraperitoneal injections on days 5, 8, 11, 14, 21, 28, 35, and 42. Results: This nanoparticle-mediated combination therapy is effective at controlling growth of treated and distant untreated tumors, enhancing animal survival and is the only one that led to complete destruction of both tumors in approximately 30% of treated mice. Corresponding with this improved response is a robust activation of the immune response, as manifested by increased numbers of immune cells along with a transcriptional signature of both innate and adaptive immunity within the tumor. Furthermore, mice treated with this combinatorial therapy display immunological memory response when rechallenged by the same cancer cells, preventing tumor engraftment.Conclusions: Our results strongly attest to the efficacy and validity of combining nanoparticle-enhanced radiotherapy and simultaneous blockade of multiple immune check point receptors and provide pre-clinical rationale for investigation into its translation into human patients.
Association between remnant cholesterol and prediabetes and diabetes : a study based on coronary heart disease angina pectoris population
Authors: Wang Yang , Liu Yijia , Yang Rongrong , Li Zhu , Su Jinyu , Yang Tong , Ma Mei , Pan Guangwei , Wang Xianliang , Li Lin , Yu Chunquan 
Published: May 2022

Abstract
Background
It has been reported that remnant cholesterol (RC) is associated with coronary artery disease (CAD) and diabetes mellitus(DM). However, there is limited evidence on the association between RC and glucose metabolism status in coronary heart disease (CHD) angina pectoris patients.
Methods
The study collected 11557 patients who were diagnosed with CHD angina pectoris in Tianjin, China. Participants were divided into normal glucose regulation (NGR), prediabetes (Pre-DM) and diabetes mellitus (DM) according to glucose metabolism status. RC was calculated as total cholesterol (TC) minus low-density lipoprotein cholesterol (LDL-C) minus low-density lipoprotein cholesterol (HDL-C). Linear regression analysis was used to explore the relationship between glucose metabolism [fasting blood glucose (FBG) and hemoglobin A1c (HbA1c)] and RC. Logistic regression was performed to analyze the relationship between the RC and Pre-DM and DM.
Results
Among all participants, 5883 (50.9%) were in DM status, 4034 (34.9%) wre in Pre-DM status and 1640 (14.2%) were in NGR status. FBG levels and HbA1c levels were positively related with RC in all participants (P < 0.001). NGR was used as a reference, multi-adjusted showed that RC was significantly associated with Pre-DM [Odds ratio (OR): 1.365; 95% confidence interval (CI) 1.290–1.672; P < 0.001] and DM (OR:1.468; 95% CI 1.290–1.672; P < 0.001). When considering RC as categorical variables (tertiles), using T1(< 1.1) as a reference, T3 had the highest relationship between RC and Pre-DM and DM in crude modle and multi-adjusted modle. The association between RC and pre-DM [(OR: 1.442; 95% CI 1.225–1.696) vs. (OR: 1.264; 95% CI 1.052–1.518)] and DM [(OR: 1.460; 95% CI 1.245–1.713) vs. (OR: 1.406; 95% CI 1.181–1.673)] in females was higher than males. The fully adjusted of OR value in the elderly patients was higher than that in the middle-aged patients in the Pre-DM (OR: 1.401 vs. OR: 1.360) and DM (OR: 1.485 vs. OR: 1.406). The relationship between RC and Pre-DM and DM was statistically significant by the different smoking and drinking stratification analyses (all P < 0.05).
Conclusion
The current study demonstrated that there was a significance association between RC and pre-DM and DM among CHD angina pectoris patients, and and the relationship was higher in females and elderly patients.
FGL2-targeted T cells induced tumor-specific brain resident TRM cells preventing glioblastoma recurrence
Authors: Zhao Qingnan , Kong Ling-Yuan , Jiang Shan , Tian Xiangjun , Wang Jing , Hashizume Rintaro , Hu Jiemiao , Jia Zhiliang , Fowlkes Natalie , Yan Jun , Xia Xeuqing , Yi Sofia , Dao Long , Masopust David , Okada Hideho , Heimberger Amy , Li Shulin 
Published: May 2022

Abstract
Tissue-resident memory T cells (TRM) specific to previously encountered pathogens have been characterized, but tumor-specific TRM cells in brain have not been reported in the literature. We discovered that T cells armed with FGL2-blocking single-chain variable fragments (T-αFGL2) are able to induce tumor-specific CD8+TRM cells, which prevent glioblastoma recurrence, a major obstacle in achieving long term survivors. These tumor-specific CD8+TRM displayed a unique highly expanded T cell receptor repertoire distinct from that found in peripheral tissues. Notably, these CD8+TRM cells could be transplanted into the brains of either immunocompetent or T cell deficient naïve mice, transforming them to become immune reactive to tumor cells. The mechanism study found T-αFGL2 therapy boosted CD69+CD8+ memory T cells population in tumor bearing brains, which depend on CXCL9/10-CXCR3 signaling. These findings are the first to show tumor-specific brain resident CD8+TRM generation via adoptive cellular treatment and may have promising implications for cancer immunotherapy.
Comparison of the Effects of the Three Methods of Massage, Selected Yoga Exercises and Massage-Yoga Combination on the Depression in Elderly Women
Authors: Elham Piri , Ghasemi Behnam , Shafizadeh Ali 
Published: May 2022

Abstract
Purpose
The present study aims to compare the effects of massage, selected yoga exercises and massage-yoga combination methods on the depression in depressed elderly women.
Methods
According to the research criteria, 60 available samples were selected and randomly divided into three experimental groups and single control one. The Beck Depression Inventory (BDI) was completed by the examinees before the intervention, two months after its beginning and one month after ending. Each experimental group received one of the massage interventions, selected yoga exercises and massage-yoga combination for eight weeks, three sessions per week and for a period of 40 minutes per session. The data were analyzed using 4 (group) × 3 (measurement) factorial analysis of variance method (ANOVA) and Bonferroni post hoc test was employed to compare the intragroup differences in the measurement steps and intergroup ones. The significance level of alpha was considered to be 0.05.
Results
The results showed that there was a significant difference in the level of depression within the three experimental groups of massage (0.001), yoga (0.00) and combination (0.00). There was also a significant difference between the four groups in the depression variable (0.001).
Conclusion
The results indicated that all three intervention methods of massage, selected yoga exercises and a combination of massage and yoga improve the depression in depressed elderly women. However, there was a significant difference between the three approaches and the massage-yoga combinative exercise program was found to have the greatest effect on improving the depression in depressed elderly women.