bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stem cells reverse the pathophysiology of cerebral malaria induced by Plasmodium berghei ANKA
Authors: Thakur Reva Sharan , Tiwari Mrinalini , Chauhan Rubika , Kalkal Meenu , Chaudhary Amrendra , Chattopadhyay Debprasad , Das Jyoti 
Published: July 2022

Abstract
Cerebral malaria-associated over expression of pro-inflammatory cytokines and chemokines ultimately results in the up-regulation of adhesion molecules in the brain endothelium leading to sequestration of mature parasitized RBCs in the brain. The high-parasitic load subsequently results in increased mortality or development of neurological symptoms within a week of infection. Studies in the human and experimental cerebral malaria have implicated the breakdown of the integrity of blood-brain barrier during the lethal course of infection, cerebral dysfunction, and fatal organ pathologies that result in multi-organ failure.In the present study, using Plasmodium berghei ANKA (PbANKA) as a mouse model and in vitro conditions, we have investigated the effect of MSCs to attenuate Cerebral malaria pathogenesis by diminishing the effect of inflammation altered organ morphology, reduced parasitemia and increased survival of the mice. MSCs are also validated for their role in preventing BBB dysfunction and reducing malarial toxins. It was observed that administration of MSCs significantly reduced parasitemia and increased survival in PbANKA infected mice. It was further demonstrated that MSCs play a significant role in reversing neurological complexities associated with cerebral malaria. Infusion of MSCs in infected mice decreased hemozoin deposition, oedema and heamorrhagic lesions in vascular organs. MSCs administration also preserved the integrity of the blood-brain barrier and reduced neural inflammation. Taken together, our results demonstrate the potential of MSCs as an emerging anti-malarial candidate.
Assessing Spanish Health Literacy and Cervical Cancer Knowledge, Attitudes, and Behaviors in a Student-Run Free Clinic
Authors: Jones Nat C. , Fusani Anna Ulyanenkova , Cox Kaitlyn Maria , Martinez Valeria Pereira , Guerra Lucy , Gonzalez Eduardo 
Published: July 2022

Abstract
Background
While the incidence of cervical cancer continues to decrease, there is a significant discrepancy in incidence rates and screening behaviors among Hispanic and non-Hispanic white patients in the United States. This project examines the relationship between Spanish health literacy and cervical cancer screening knowledge, attitudes, and practices among native Spanish-speaking patients at risk for cervical cancer at the USF BRIDGE Healthcare Clinic, a student-run free clinic in Tampa, Florida.
Methods
Spanish-speaking patients ≥ 21 years (n = 34) participated in a quality improvement project that included an assessment of Spanish health literacy and a written survey on cervical cancer knowledge. Chi-squared tests were performed to assess potential relationships between health literacy and cervical cancer knowledge, attitudes, health behaviors, and demographics.
Results
Seven participants (20.6%) scored between 0–14 on the SAHL-S, indicating inadequate health literacy. A significant difference in cervical cancer health knowledge was found between patients with adequate health literacy compared to patients with inadequate health literacy (p = 0.002).
Conclusions
There is a potential association between low Spanish health literacy and subsequent poorer understanding of cervical cancer in BRIDGE patients. This implies that patients of low health literacy may have poorer comprehension of other aspects of their care beyond cervical cancer screening. Strategies are discussed to improve communication with BRIDGE patients of low Spanish health literacy that may be applicable to other patient populations.
Adherence to Iron and Folic Acid Supplementation and Associated Factors among Mothers receiving Antenatal Care in Lira District, Uganda: A cross-sectional descriptive study
Authors: Ssewankambo Usaama , Nakaziba Rebecca 
Published: July 2022

Abstract
Background
Prenatal Iron and Folic Acid supplementation is an economical way of decreasing iron and folic acid deficiency anaemia amongst expectant mothers in resource limited countries like Uganda. This study was geared toward assessing the levels of compliance to Iron and Folic Acid Supplements as well as the associated factors amongst mothers receiving prenatal services in Lira district, Uganda.
Methods
252 pregnant mothers were enlisted in a cross-sectional study at Lira Regional Referral Hospital at the antenatal clinic. Adherence levels to Iron and Folic Acid Supplements were evaluated using a visual analogue scale and the associated factors were compiled through an interviewer-administered questionnaire. The findings were analyzed using SPSS software and reported in tables.
Results
Only 46% of the mothers attending the antenatal clinic adhered to iron and folic acid supplements over 30-day period of the previous month before the study. Participants who had good knowledge of Iron and Folic Acid Supplements before recruitment (OR 1.49 95% CI 1.12–1.97), using any reminder technique (OR 1.05; 95% CI 1.02–1.09) and being supported by their partner or relatives (OR 1.56, 95% CI 1.07–2.29) were more likely to have good adherence. Forgetfulness and fear of taking too many tablets were the main reasons for missed iron and folic acid supplements.
Conclusions
There was low adherence to iron and folic acid supplements among mothers attending antenatal clinic in Lira district. We recommend further investigations into the hindrances to adherence and provision of comprehensive health education to pregnant mothers.
Predictors of outcome after endovascular treatment for Tandem Occlusions: a single center retrospective analysis
Authors: Enriquez Brian Anthony , Nome Terje , Nome Cecilie G. , Tennøe Bjørn , Lund Christian G. , Beyer Mona K. , Skjelland Mona , Aamodt Anne Hege 
Published: July 2022

Abstract
Background
The endovascular treatment procedure in tandem occlusions (TO) is complex compared to single occlusion (SO) and optimal management remains uncertain. The aim of this study was to identify clinical and procedural factors that may be associated to efficacy and safety in the management of TO and compare functional outcome in TO and SO stroke patients.
Methods
This is a prospective, observational single center study of medium (MeVO) and large vessel occlusion (LVO) of the anterior circulation. Clinical, imaging, and interventional data were analyzed to identify predictive factors for symptomatic intracranial hemorrhage (sICH) and functional outcome after endovascular treatment (EVT). Functional outcome in TO and SO patients was compared.
Results
Of 662 anterior circulation stroke patients with MeVO and LVO stroke, 90 (14%) had TO. Stenting was performed in 73 (81%) of TO patients. Successful reperfusion with modified thrombolysis in cerebral infarction (mTICI) ≥ 2b was achieved in 82 (91%). SICH occurred in seven (8%). The strongest predictors for sICH were diabetes mellitus and number of stent retriever passes. Good functional clinical outcome (mRS ≤ 2) at 90-day follow up was similar in TO and SO patients (58% vs 59% respectively). General anesthesia (GA) was associated with good functional outcome whereas hemorrhage in the infarcted tissue, lower mTICI score and history of smoking were associated with poor outcome.
Conclusions
The risk of sICH was increased in patients with diabetes mellitus and those with extra stent-retriever attempts. Functional clinical outcomes in patients with TO were comparable to patients with SO.
WITHDRAWN: Genome-wide surveying of cucumber (Cucumis sativus L.) lines for idetifying loci of ZYMV resistance
Authors: Gokbulut Tarik , Fidan Hakan , Fırat Ahmet Fikret , Coskun Omer Faruk , Pinar Hasan , Gulsen Osman 
Published: July 2022

Abstract
Control of virus diseases such as zuchini yellow mosaic virus (ZYMV) of cucumber causing major yield and quality losses is impossible by using any chemical pesticide except for using elimination of vectors of viruses and resistant cultivar. The objectives of this study were to investigate structure of genetic resistance to ZYMV of cucumber for marker-assisted selection (MAS). Forty-eigth resistant and 48 susceptible lines were selected from a germplasm of 600 cucumber lines. Bulk DNAs of resistant and susceptible lines were screened to find candidate marker(s) by using ISSR, RAPD and a cleaved amplified polymorphism (CAP) primers, which targeted over 5000 loci of cucumber. Candidate markers later were applied to 96 lines including 48 resistance and susceptible lines to find associating markers. A total of 54 candidate marker loci were produced, which were clustered into two groups based on Dice’, average distance, simple matching and correlation similarity matrices. In the association analyses by using TASSEL software, associating markers were detected. These markers were then subjected to backward regression analysis by SAS program. The results explained %78 of total variation for ZYMV. The remaining 22% of the variation was possibly due to presence of additional gene(s) and environmental effect that caused lower repeatability.
The impact of income gap on the CO2 emission and grey trend prediction
Authors: Cai Kai , Pan Xiaoying , Wu Lifeng 
Published: July 2022

Abstract
The CO2 emissions of urban and rural are different. The research on the life and production of urban and rural residents can help to adjust the carbon emission policy more accurately. The income gap between urban and rural residents is an important embodiment of the differences in the life and production of urban and rural. Taking the income gap between urban and rural residents as an input variable, the non-equidistant grey model with conformable fractional accumulation (CFNGM(1,1)) is used to predict the CO2 emissions of six provinces in China. The CFNGM(1,1) model is a novel grey prediction model, which satisfies that the input sequence is non-equidistant sequence. The predicted results show that there is a large income gap in East China, and the CO2 emissions are increasing. The income gap in Northwest China is relatively smaller, and the CO2 emissions are gradually reduced. It is concluded that there is a positive correlation between the income gap and the CO2 emission. Based on the cases analysis, it is verified that the life and production of urban and rural have different effects on CO2 emission. Finally, based on the research results, the suggestions for sustainable development of environment and economy are put forward.
The health and climate benefits of economic dispatch
in China’s power system
Authors: Luo Qian , Garcia-Menendez Fernando , Yang Haozhe , Deshmukh Ranjit , He Gang , Lin Jiang , Johnson Jeremiah 
Published: July 2022

Abstract
China’s power system is highly regulated and uses an “equal-share" dispatch approach. However, market mechanisms are being introduced to reduce generation costs and improve system reliability. Here, we quantify the climate and human health impacts brought about by this transition, modeling China’s power system operations under economic dispatch. We find that significant reductions in mortality related to air pollution (11%) and CO2 emissions (3%) from the power sector can be attained by economic dispatch, relative to the equal-share approach, through more efficient coal-powered generation. Additional health and climate benefits can be achieved by incorporating emission externalities in electricity generation costs. However, the benefits of the transition to economic dispatch will be unevenly distributed across China and may lead to increased health damages in some regions. Our results show the potential of dispatch decision-making in electricity generation to mitigate negative impacts of power plant emissions with existing facilities in China.
Characterization, Seasonal Variations, Source Apportionment and Health Risk Assessment of Heavy Metals in PM2.5 in the Urban Site of Agra, India
Authors: Sah Dinesh 
Published: July 2022

Abstract
The present study evaluated the concentration, seasonal variations, source identification and health risk through inhalation of heavy metals (V, Cr, Mn, Fe, Ni, Cu, Zn, As, Cd, Si and Pb) in fine particulate matter. Samples of PM2.5 were collected from an urban site in Agra (27°10′N, 78°05′E), India during January 2018 to January 2019. A total of 48 samples of PM2.5 were collected using Fine Particulate Sampler (Envirotech APM 550) set at a flow of 16.6 L min− 1 on 47 mm quartz fiber filters (Pallflex, Tissuquartz) for 24 h. Heavy metals were determined by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) after acid digestion. The average PM2.5 mass concentration was 107 ± 57.85 µg m− 3 which has been compared with NAAQS and WHO limit was found to be higher. Seasonal variation of PM2.5 showed: winter > post-monsoon > summer > monsoon. The average concentration of heavy metals lied within the range of 4.92 to 505.38 ng m− 3. Among heavy metals, the high concentration belonged to Si (505.38 ng m− 3) and Fe (281.23 ng m− 3). The highest metal concentration occurred in the winter season followed by post-monsoon, summer and monsoon seasons. The enrichment factor was used to estimate the main sources of the metals in PM2.5. Two factors were identified using Principal Component Analysis (PCA) at the urban site including resuspended road dust, vehicular activities, solid waste incineration, industrial emission and construction activities. To assess the health risks through inhalation exposure pathway hazard quotient (HQ), hazard index (HI) and carcinogenic risk (CR) were estimated. The carcinogenic risks of Pb (for both children and adults) Cd (for children) were lower than the precautionary criterion (1×10− 6), while Cd for children and As, Cr and Ni for both children and adults were higher than 1×10− 6. The non-carcinogenic risks of As, Cd, Cr, Mn and V for both children and adults were lower than the safe level of 1, whereas Ni for both children and adults were higher than the safe level of 1. The HI values of all heavy metals for both children and adults were higher than the safe level of 1 indicating a non-carcinogenic adverse health effect. The results of the present study impart information for the behaviour and risk mitigation of heavy metals.
Sustainable-use marine protected areas to improve human nutrition
Authors: Viana Daniel , Gill David , Zvoleff Alex , Krueck Nils , Zamborain-Mason Jessica , Free Christopher , Shepon Alon , Mascia Michael , Grieco Dana , Schmidhuber Josef , Golden Christopher 
Published: July 2022

Abstract
Coral reef fisheries are a vital source of nutrients for thousands of nutritionally vulnerable coastal communities around the world. Here, we evaluated the potential effects of expanding sustainable-use marine protected areas (MPAs) to improve the nutrition of coastal communities. Using information from underwater visual surveys from 2,518 sites located in 53 countries, we developed a Bayesian hierarchical model to estimate the average effect of existing sustainable-use MPAs reef fish biomass and explored how that may alter fish catch, and the nutrients supplied to local communities. We then estimated the potential nutritional benefits of expanding sustainable-use MPAs to all non-MPA coral reefs globally. We found that existing sustainable use MPAs have on average 15% more biomass than open access reefs. Translating this into catch, we estimated that expanding sustainable-use MPAs could increase catch potential by 0-20%, which could prevent 0.53-1.95 million cases of inadequate micronutrient intake globally, a fraction of the people who would continue to be sustained by this foundation of coastal food systems. Our study estimates the potential nutritional benefits of expanding sustainable-use MPAs and pinpoints locations with the greatest potential to reduce inadequate micronutrient intake levels, critical knowledge given the strong international movement to cover 30% of our oceans with MPAs by 2030.
Towards Safe Cholinergic Modulators: Investigating the Action of Berberine Nanoparticles
Authors: Leimann Fernanda Vitória , Souza Luma Borges , Oliveira Byanca Pereira Moreira , Rossi Bruna Franzon , Silva Patrícia Sabino , Shiraishi Carlos Seiti Hurtado , Foleis Vanessa Kaplum , Abreu Rui Miguel , Pereira Carla , Barros Lillian , Peron Ana Paula , Ineu Rafael Porto , Oechsler Bruno Francisco , Sayer Claudia , Araújo Pedro Henrique Hermes , Gonçalves Odinei Hess 
Published: July 2022

Abstract
Researchers have concentrated efforts in the search for natural-based reversible inhibitors for cholinesterase enzymes because they may play a key role in the treatment and cure of degenerative diseases like Alzheimer’s Disease. Diverse alkaloids from plants can inhibit the action of acetylcholinesterase enzyme and, among them, berberine is a promising bioactive. Although the mechanism of action of berberine still needs to be fully elucidated, the major concern lies in its poor water solubility and consequent low bioavailability. The solid dispersion technique is a well-known procedure to improve the water affinity of hydrophobic substances, however, the obtention of berberine solid dispersions is yet to be truly investigated. It is also of key importance to elucidate the safety aspects of the berberine-loaded solid dispersions and a range of tests can be applied, such as in vitro cytotoxicity in cell lines and in vivo tests using model organisms. In this work, berberine-loaded solid dispersions were obtained and evaluated as inhibitor of acetylcholinesterase enzyme (AChE). A polypropylene glycol / polyethylene glycol triblock copolymer was used in the solid dispersion formation. In a first step, the influence of berberine on AChE was investigated by docking simulation, showing the active sites in which berberine may anchor to the enzyme. The enzymatic reactions involved in the action of AChE on the acetylcholine were investigated by kinetic modeling. In vitro assays were carried out to confirm the enzymatic kinetics of AChE in the presence of berberine. berberine nanoparticles exerted an improved cytotoxic effect on tumoral cells demonstrating the improvement in water affinity. In vivo assays in Allium cepa were implemented showing that no cytotoxicity or genotoxicity is expected for the berberine solid dispersion. Berberine-loaded solid dispersions may represent a significant step towards safe nanostructures to be used in the treatment of neurodegenerative diseases.
Transcriptome analysis reveals the high temperature-induced damage is a significant factor affecting the osmotic function of gill tissue in cold-water fish
Authors: Yang Shiyong , Li Datian , Feng Langkun , Zhang Chaoyang , Xi Dandan , Liu Hongli , Yan Chaozhan , Xu zihan , zhang Yujie , Li Yunkun , Yan Taiming , He Zhi , Wu Jiayun , Gong Quan , Du Jun , Huang Xiaoli , Du Xiaogang 
Published: July 2022

Abstract
Background
Maintaining osmotic balance plays an important role in the normal operation and survival of cold-water fish. High water temperature has been proven to reduce the activity of NA+-K+-ATPase in the gill. However, the mechanism of megatemperature affect gill osmoregulation has not been fully elucidated.
Results
In this study, A.baerii, as a kind of iconic cold-water fish, was used to analyze histological alterations, plasma ion content, and transcriptional responses of gill of A.baerii, subjected to 20℃, 24℃and 28℃. We found that ROS levels in gill tissue increased and the gill damage deteriorated along with the temperature rising. Moreover, plasma Cl− levels at 28℃ were much lower than those in the other two groups, and there were no significant differences in Na+ and K+ ions among all trials. At the same time, the transcriptome data indicated that there were significant changes in injury-related pathways and genes at 24℃and 28℃. Meanwhile, GO enrichment analysis showed a large number of genes related to ion transport function changed with temperature increasing during cell processes. We discovered that as temperature increased, the expression of genes that regulate ion outflow (OCLN) and active ion absorption (AE2, NHE3, CLC-2) increased, while NKA-a expression decreased. Furthermore, the changes in osmotic regulation genes and the degree of tissue damage at 28℃ were significantly higher than those in the other two groups, indicating that the degree of heat stress-induced injury affects the osmotic regulation function of gill tissue.
Conclusions
this study reveals for the first time that the tissue damage caused by high temperature affects the function and the response mechanism of the osmotic regulation in gill of cold-water fish. It provides reference data for the study on the physiological function of cold-water fish under global warming.
Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule symbiosis establishment
Authors: Liu Zhijian , Kong Xiangying , Long Yanping , Zhang Hong , Jia Jinbu , Qiu Li-juan , Zhai Jixian , Yan Zhe 
Published: July 2022

Abstract
Legumes form symbiosis with rhizobium leading to the development of nitrogen-fixing nodules. By integrating single-nucleus and spatial transcriptomics, we established a cell atlas of soybean nodules and roots. In central infected zone of nodule, we found that uninfected cells specialize into functionally distinct sub-groups during nodule development and revealed a transitional subtype of infected cells with enriched nodulation-related genes. Overall, our results provide a single-cell perspective for understanding rhizobium-legume symbiosis.
Critical care nurses' knowledge and attitudes toward using ventilator waveform monitoring to detect patient-ventilator asynchrony: A cross-sectional online survey
Authors: Abdelgawad Mohamed Ezzelregal , Mohamed Farida Khalil , Ghoneam Mohammed 
Published: July 2022

Abstract
Background
Most mechanically ventilated patients will be exposed to some asynchrony with the ventilator. Inability to detect and manage patient-ventilator asynchrony (PVA) as early as possible may lead to poor outcomes, including, but not limited to, prolonged mechanical ventilation, extended ICU stay, and higher mortality. Critical care nurses as primary health care providers may achieve significant participation in the timely detection of PVA. Waveform monitoring is a non-invasive and reliable method, but it is also regarded as a challenging task even for skilled clinicians. The aim of the study is to assess the knowledge level and attitude of critical care nurses in Egypt regarding the use of ventilator waveform monitoring to detect PVA.
Methods
A cross-sectional online survey was carried out in three intensive care units in three hospitals in Alexandria, Egypt. The questionnaire comprised four parts to evaluate critical care nurses' level of knowledge and attitude regarding ventilator waveform monitoring and assessed their ability to detect PVA.
Results
Most nurses (88.1%) have poor knowledge levels and negative attitudes (93.1%) toward using waveform monitoring to detect PVA. A significant relationship was found between nurses' knowledge of ventilator waveform monitoring and previous training programs on mechanical ventilation (P = 0.031). In addition, a significant relationship between nurses' attitude toward ventilator waveform monitoring and their level of education (P = 0.002), and their attendance in previous courses containing waveform analysis (P = 0.020) was noted. A highly significant relation (P = 0.000) was detected between nurses' level of knowledge and their attitude toward ventilator waveform monitoring.
Conclusions
The majority of the critical care nurses have poor knowledge and negative attitudes regarding using ventilator waveform monitoring to detect PVA. Previous training on mechanical ventilation and attendance of courses on ventilator waveform analysis was found to be significantly correlated with nurses' level of knowledge and attitude regarding ventilator waveform monitoring.