bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selecting logging, and old secondary vegetation conserve biodiversity and composition of bacteria and arbuscular mycorrhizal fungi in a community-based forestry in the Maya Forest
Authors: Puch-Hau Carlos , Polo-Marcial Martín H. , Quintanilla-Mena Mercedes , Cañizares-Martínez Alejandra , Adame-Castro Diana E. , Pérez-Garfias Brisni Z. , Granados-Puerto Silvia G. , González-Godoy Edgar , Márquez-Santos Magdalena , Lara Luis Alberto 
Published: June 2022

Abstract
The impacts of selective logging on arbuscular mycorrhiza and bacteria of tropical forests are little known globally. In this study, we analysed and compared the composition and diversity of bacteria and arbuscular mycorrhizal fungi (AMF) in a forest with sustainable management (stands with 6-years-old after selective logging) compared with a conserved forest and secondary vegetation recovered from agriculture (20-years-old) in Nuevo Becal, Campeche, Mexico. The analyses were based on environmental DNA sequences using Illumina miseq platform and morphological diversity of AMF glomerospores. We found 30 species of virtual AMF taxa (10 potential new virtual taxa) based on DNA sequences, 22 AMF species based on morphology, and 3,963 amplicon sequence variants (ASVs) for bacteria. For AMF, the most abundant order (in the whole study) were the Glomerales (with higher significant abundance in secondary vegetation), followed by Gigasporales and Diversisporales, while for bacterial phylum, Proteobacteria and Actinobacteria were highly represented, followed by Acidobacteria, Planctomycetes, Nitrospirae, Bacteroidetes, and Gemmationonadetes. Actinobacteria showed significantly higher relative abundance in conserved forests, Chloroflexi in managed forests, and Verrucomicrobia in secondary vegetation. Bacterial and glomerospores community composition did not showed significant differences among the three sites analysed. However, AMF VT community composition were significant different between the conserved forest and secondary vegetation. Forest management showed higher bacteria and glomerospores diversity (Shannon index) compared to the conserved forest and secondary vegetation. Our study showed that stands 6 years after selective logging and old secondary vegetation conserved an important bacteria and AMF species compared with conserved forests.
A Real-world Study on a Large Retrospective Cohort of Castleman Disease from a Single Center
Authors: Guo Meiyu , Nong Lin , Wang Mingyue , Zhang Yang , Wang Lihong , Sun Yuhua , Wang Qingyun , Liu Huihui , Ou Jinping , Cen Xinan , Ren Hanyun , Dong Yujun 
Published: June 2022

Abstract
Castleman disease(CD) is a rare lymphoid tissue proliferative disease with an increasing focus on etiology and treatment in recent years. The consensus on CD diagnosis and treatment has been published by several organizations, playing an important role in promoting cooperation on CD managements and investigations among different teams. Based on the last 15-years retrospective real-world data from Peking University First Hospital (PKUFH), we re-classified and re-evaluated the clinical and pathological information of patients with pathologically suspected diagnosis of CD. A total of 203 patients were included in our study, in which the diagnosis of CD was confirmed in 189 cases, including 118 patients with unicentric CD (UCD, n = 118, 62.4%) and 71 patients with multicentric CD (MCD, n = 71, 37.6%). 44.1% (n = 52) of UCDs in our cohort were complicated with Paraneoplastic Pemphigus (PNP). The treatment of UCD is mostly surgical resection, with a 5-year overall survival(OS) 88.1%. Patients with PNP had a poorer prognosis than those without PNP [82.9% (95% CI 123–178) vs 92.8% (95% CI 168–196), log-rank P = 0.041). The rate of concurrent systemic symptoms was 74.6% (n = 53), and renal involvement occurred in 49.3% (n = 35) MCD patients. The MCD treatments were mainly chemotherapy regimens, with a 5-year OS of 77.6% (95% CI, 143–213). In conclusion, UCDs have a better overall prognosis than MCDs. But the prognosis of those complicated with PNP was poor. Differential diagnosis of MCD is difficult. MCD treatment in China is heterogeneous. The inaccessibility of anti-IL-6-targeted drugs in China may contribute to the poor prognosis of MCD.
Evaluation of the physicochemical and antimicrobial properties of the polyvinyl alcohol-mulberry leaf extract blend film
Authors: Chowdhury Sujan , Zaidi Nur Syaliani Binti Mohamed Rafflisman , Mah Shee Keat 
Published: June 2022

Abstract
In this study, mulberry (Morus nigra sp.) leaves extract (MN) was used as a structure modifier and antimicrobial agent in poly(vinyl alcohol) (PVA) blend films. We prepared PVA/MN blend films by solution casting at various concentrations. The SEM micrographs showed that PVA_MN75 has a smooth and continuous cross-sectional morphology with better compatibility. Fourier-transform infrared spectroscopy results suggest that a specific concentration of MN in PVA films facilitates a physical interaction that improves mechanical and thermal properties. The tensile strength of the films increased from 1.886 MPa to 2.907 MPa after adding mulberry leaf extract. Furthermore, the films were assessed for their solubility in water (12.116-15.833 %), absorption (9.800-21.610 %), and moisture content (6.530-14.257), which further demonstrates that blend films exhibit lower water solubility. Moreover, the soil burial biodegradation test indicates that PVA/MN film biodegradability is strongly affected by its MN concentration. Upon soil burial, all films showed an increase in biodegradability of 29% as burial time has prolonged in both soil and compost for thirty seven (37) days. Enhanced antibacterial activity has been observed in the PVA/MN film-forming solution and can be considered a promising substitute for polluting plastic packaging.
Dissections and Elastic Recoil Post Chocolate™ Balloon Catheter Treatment in Femoropopliteal Artery Lesions: Visions from Intravascular Ultrasound and 12- month following-up results
Authors: Zou Yuchi , Cheng Jun , Wang Xuehu , Song Wei , Dai Yuanbin , Zhao Yu 
Published: June 2022

Abstract
Purpose
Situations of vessel wall in IVUS images after Chocolate balloon dilatation remains unknown and 12-month following-up results would be performed.
Materials and Methods
25 patients were enrolled in this observational study. Angiography and intravascular ultrasound were performed before Chocolate balloon inflation and post inflation. Dissections were quantified and classified according to the iDissection classification on intravascular ultrasound (IVUS) and National Heart, Lung, and Blood Institute classification on angiography. The elastic recoil rate, plaque burden and luminal gain were calculated in each patient too. Doppler ultrasound was presented in 30days, 3months, 6months and 12months each patient during follow-up.
Results
The mean lesion length was 168.3 ± 26.6 mm and arc of calcium was 115.1 ± 84.6°. 7 of the 25 patients required stents because of flow-limiting dissections and another 8 patients used stents with residual narrowing > 30%. Post Chocolate balloon angioplasty, the elastic recoil rate was 32.4 ± 13.1% and luminal gain was 6.1 ± 2.8mm2. The plaque burden volume reduction was 7.0 ± 4.6%. Diameters on cine were 5.1 ± 0.7mm and 5.9 ± 0.6mm on IVUS. Total number of dissections were 2.9 times (114/40) on IVUS images than cine film, more non-flow-limiting dissections observed on IVUS compared cine (102/31). 2 patients lost follow-up because of death, primary patency in patients was 52.2% (12/23) and secondary patency was 82.6% (19/23) in 12-month.
Conclusion
Vessel injury from Chocolate balloon angioplasty and diameter in femoropopliteal artery lesions were grossly underestimated on angiography. Severe elastic recoil performed in most of patients according to IVUS images after Chocolate. Further randomized controlled trials are needed to support their use.
Can we predict who will benefit most from biologics in severe asthma? A post-hoc analysis of two phase 3 trials
Authors: Chen Wenjia , Reddel Helen K. , FitzGerald J Mark , Beasley Richard , Janson Christer , Sadatsafavi Mohsen 
Published: June 2022

Abstract
Background: Individualized prediction of treatment response may improve the value proposition of advanced treatment options in severe asthma. This study aimed to investigate the combined capacity of patient characteristics in predicting treatment response to mepolizumab in patients with severe asthma. Methods: Patient-level data were pooled from two multinational phase 3 trials of mepolizumab in severe eosinophilic asthma. We fitted penalized regression models to quantify reductions in the rate of severe exacerbations and the 5-item Asthma Control Questionnaire (ACQ5) score. The capacity of 15 covariates towards predicting treatment response was quantified by the Gini index (measuring disparities in treatment benefit) as well as observed treatment benefit within the quintiles of predicted treatment benefit. Results: There was marked variability in the ability of patient characteristics to predict treatment response; covariates explained greater heterogeneity in predicting treatment response to asthma control than to exacerbation frequency (Gini index 0.35 v. 0.24). Overall, the average reduction in exacerbations was 0.90/year (95%CI, 0.87‒0.92) and average reduction in ACQ5 score was 0.18 (95% CI, 0.02‒0.35). Among the top 20% of patients for predicted treatment benefit, exacerbations were reduced by 2.23/year (95% CI, 2.03‒2.43) and ACQ5 score were reduced by 0.59 (95% CI, 0.19‒0.98). Among the bottom 20% of patients for predicted treatment benefit, exacerbations were reduced by 0.25/year (95% CI, 0.16‒0.34) and ACQ5 by -0.20 (95% CI, -0.51 to 0.11).Conclusion: A precision medicine approach based on multiple patient characteristics can guide biologic therapy in severe asthma, especially in identifying patients who will not benefit as much from therapy. Patient characteristics had a greater capacity to predict treatment response to asthma control than exacerbation.Trial registration: ClinicalTrials.gov number, NCT01691521 (registered September 24, 2012) and NCT01000506 (registered October 23, 2009).
LIX1L promotes EMT and EGFR-TKIs resistance via nucleolin-mediated ribosomal RNA synthesis in NSCLC
Authors: Li Minglei , Zhang Jiaxi , Meng Xue , Liu Bei , Xie Shelly , Liu Fang , Yao Demin , Zhang Jingguo , Shen Haitao , Xing Lingxiao 
Published: June 2022

Abstract
Limb expression 1-like protein (LIX1L) might be an RNA binding protein involved in post-transcriptional regulation. However, little is known regarding the biological function and mechanism of LIX1L in cancer cells. Here we demonstrate a clear correlation between LIX1L expression and EMT markers in 81 NSCLC tissues and TCGA database, suggesting that LIX1L is a mesenchymal marker. Besides, LIX1L expression is obviously elevated in TGFβ1-induced EMT NSCLC cells, and enhances cell migration, invasion, anoikis resistance, EGFR-TKIs resistance and proliferation. Interestingly, the increased LIX1L expression prominently localizes to the nucleoli, where it physically interacts with the key ribosome biogenesis regulator NCL protein, inducing rRNA synthesis in EMT NSCLC cells. NCL knockdown or inhibition of rRNA synthesis reverses the enhanced EMT functions and proliferation ability caused by LIX1L overexpression in NSCLC cells, indicating that NCL expression and rRNA synthesis participates in LIX1L-mediated biological functions during EMT. Collectively, our findings suggest that the LIX1L-NCL-rRNA synthesis axis is a novel EMT-activated mechanism. Targeting the pathway might be a therapeutic option for EMT and EGFR-TKIs resistance in NSCLC.
The Impact of Vitamin D and Cognitive Function on All-Cause Mortality Among Older Chinese Adults: A Population-based Longitudinal Study
Authors: Dai Miao , Song Quhong , Wang Xiang , Lin Taiping , Liang Rui , Jiang Tingting , Shu Xiaoyu , Yue Jirong 
Published: June 2022

Abstract
Background
While both vitamin D deficiency and cognitive impairment have individually been linked to a greater risk of all-cause mortality, the combined effects of these two different conditions have not previously been explored in this context. We aimed to investigate the combined impact of vitamin D levels and cognitive impairment on all-cause mortality in older adults.
Methods
Analyzed data were collected from community-dwelling adults ≥ 65 years of age that were enrolled in the Chinese Longitudinal Healthy Longevity Survey for whom follow-up data were available over a 7-year period (n = 2,154). The Mini-Mental Status Examination (MMSE) was used to detect cognitive impairment, while plasma 25-hydroxyvitamin D (25(OH)D) concentrations were used to measure vitamin D concentrations. The associations between baseline cognitive function and vitamin D were assessed using bivariate logistic regression models, while the relationships between vitamin D levels, cognitive function, and all-cause mortality were assessed with Cox proportional hazards models. Joint effect testing to explore interactions between vitamin D levels and cognitive function.
Results
Of the included study participants, 839 (39.0%) died over the course of follow-up. Individuals in the lowest 25(OH)D quartile exhibited a 1.61-fold higher risk of cognitive impairment as compared to participants within the highest quartile. A negative dose-response relationship was observed between 25(OH)D levels and odds of all-cause mortality. Similarly, cognitive impairment was significantly related to mortality risk (HR 1.75, 95% CI: 1.49 to 2.06). Relative to participants with normal cognition in the highest 25(OH)D quartile, a negative dose-response relationship was observed between the levels of 25(OH)D and the odds of all-cause mortality. Moreover, the interaction between 25(OH)D levels and cognitive function was found to be significant with respect to the risk of mortality (P for interaction < 0.001).
Conclusions
These data suggest that lower plasma 25(OH)D concentrations are related to the development of cognitive impairment, while 25(OH)D and cognitive impairment exhibit an additive combined effect on all-cause mortality among older Chinese adults.
Gender differences in perceptions of the convenience of outdoor activity among elderly people with a physical disability
Authors: Xu Qi , Lin Youyi , He Yiqi , Zhou Xianhong , Liu Jinhai , Shen Dewang , Wu Fan , Lin Xin , Zhang Yun , Li Taibiao , Yan Tiebin 
Published: June 2022

Abstract
Objective: To clarify the relationships among health-related quality of life, social factors, behavioral factors and the self-reported convenience of outdoor activity among older Chinese people with a physical disability. Gender differences were studied.Method: A cross-sectional study of 1216 community-dwelling persons over or equal to 60 with a physical disability from Chinese Xiamen’s Xiang’an District between May and December 2019. The theory of planned behavior was applied to generate gender-specific structural equation models predicting convenience perceptions. The standardized coefficients explained the contribution of the variance.Results: The final structural models demonstrated good fit. Convenience of outdoor activity for both women and men was directly impacted by their physical functioning (βwoman = 0.641, βman = 0.675, pboth ≤ 0.01) and intention of participation in community physical activities (βwoman = 0.134, βman = 0.302, pboth ≤ 0.01) , and indirectly by social and economic factors, pain, and role emotions. Positive mental health was more influential for women, while men were more influenced by social norms in the pathway.Conclusions: Both gender models demonstrated good fit in predicting self-reported convenience of outdoor activity. Interventions targeting health-related quality of life, behavior and social factors could predict perceptions of the convenience of outdoor activities. Gender differences need to be considered.
The relative influence of habitat modification on bird assemblage in the university's domain of Notre Dame du Kasaï in Kananga, the Democratic Republic of the Congo
Authors: KAFUTSHI Robert KISASA , KUMANENGE Julien PUNGA 
Published: June 2022

Abstract
The study was conducted at the University of Notre Dame du Kasaï from 2019 to 2021, combined with observation and photography. The aim was to understand the characteristics of avifauna and the composition and diversity of species in this environment. the results of the study indicate that there are 72 species of birds representing 35 families and 12 orders. The order Passeriformes alone contains 50 or 69.4% of species and 22 or 62.9% of families. The order Accipitriformes has a single-family and four species. The other charges include one to two families with often a single species. Over time, the avian fauna of this university site has undergone many changes in its composition and diversity. The installation of the artificial eucalyptus forest associated with the various consecutive changes in habitats seems to be the main factor that modulates these characteristics. Indeed, most of the reputed forest species were observed from 2019 to 2021, coinciding with this forest's maximum vegetation cover. However, information gathered on the IUCN website reveals that the protection status of all these species comma on the common concern (LC).
Ultrastructural characterization of dark microglia during aging in a mouse model of Alzheimer’s disease pathology and in human post-mortem brain samples
Authors: St-Pierre Marie-Kim , Carrier Micaël , Ibáñez Fernando González , Šimončičová Eva , Wallman Marie-Josée , Vallières Luc , Parent Martin , Tremblay Marie-Eve 
Published: June 2022

Abstract
A diverse heterogeneity of microglial cells was previously described in Alzheimer’s disease (AD) pathology, including dark microglia, a state characterized by ultrastructural markers of cellular stress. To provide novel insights into the roles of dark microglia during aging in the context of AD pathology, we performed a quantitative density and ultrastructural analysis of these cells using high-throughput scanning electron microscopy in the ventral hippocampus CA1 stratum lacunosum-moleculare of 20-month-old APP-PS1 vs C57BL/6J male mice. The density of dark microglia was significantly higher in APP-PS1 vs C57BL/6J mice, with these cells accounting for nearly half of all microglia observed near amyloid-beta (Aß) plaques. This dark microglial state interacted more with dystrophic neurites compared to other APP-PS1 microglia and possessed glycogen granules, associated with a metabolic shift toward glycolysis, which provides the first ultrastructural evidence of their presence in microglia. Dark microglia were further observed in aging human post-mortem brain samples showing similar ultrastructural features as in mouse. Overall, our results provide a quantitative ultrastructural characterization of a microglial state associated with cellular stress (i.e., dark microglia) that is primarily restricted near Aß plaques and dystrophic neurites. The presence of this microglial state in the aging human post-mortem brain is further revealed.
Elemental Variability of PM 2.5 aerosols in Old Jeddah, Saudi Arabia
Authors: Shaltout Abdallah A. , Ali Safaa S. M. , Al-Malawi Dhaif-allah R. , Alzahrani Eman 
Published: June 2022

Abstract
Air particulate matter with a diameter ≤ 2.5 µm (PM2.5) has been assembled from the historical old Jeddah district (downtown) throughout the whole year. Additional PM2.5 aerosols have been collected during the autumn and winter seasons from another newly constructed district inside Jeddah city (Alnaeem). The annual concentrations of the total mass of the PM2.5 aerosols from old Jeddah and Alnaeem sites were found to be 43 ± 6 and 61 ± 14 µg/m3, respectively. These values are greater than the annual mass concentration of the air quality standards of the European Commission (EC, 25 µg/m3) and the world health organization (WHO, 10 µg/m3). The elemental analysis of the collected fine atmospheric aerosols has been achieved by an energy dispersive X-ray fluorescence (EDXRF) with three secondary targets (CaF, Ge, and Mo). Quantitative elemental analyses of twenty-two (22) elements have been achieved starting from the low Z element (Na) up to the high Z element (Pb). Although the old Jeddah site is not well organized, the elemental concentrations and total mass concentrations are lower than other sites. The statistical analyses including enrichment factors, the correlation analysis of Pearson, and the principal component analysis reveal more information about the source identification of the PM2.5 aerosols collected from both locations. It was recognized that the quantified elements Al, Si, K, Ca, Ti, Mn, Fe, Rb, and Sr originate from a natural source. On the other hand, the elements Ta, Br, Pb, Sc, Ni, Cu, Zn, and S originate from anthropogenic source. Finally, the elements Na, Cl, and Br come mainly from the sea spray source.
Performance Evaluation of a Pseudo Two-axis Sun Tracking System
Authors: Adibpour Saeed , Alemrajabi Ali Akbar 
Published: June 2022

Abstract
Tracking the sun is an important method for increasing the electricity generation of photovoltaic panels. Sun tracking systems are designed to track the sun with one axis or two axes, based upon solar azimuth angle and altitude angle. In this research, a pseudo two-axis sun tracker is presented and applied for installing the photovoltaic panel on. In this tracking system, one rotating motor is used instead of two, and more solar radiation is absorbed compared with common one-axis tracking systems. The results show that pseudo two-axis sun tracking system gains 2.82% more radiation than the conventional one-axis sun tracker. Through adjusting the angle two times a year, 4.01% more radiation is gained and adjusting the angle four times a year results in gaining 4.12% more radiation, while using a two-axis sun tracker results in 4.39% more radiation on the panel compared with one-axis sun tracker. The pseudo two-axis sun tracker’s performance with adjusting angle four times a year has little difference with two-axis sun tracker and due to using one motor instead of two, using a pseudo two-axis sun tracker is more economical. The percentage of increased radiation of pseudo two-axis sun tracker compared with fixed panel differs for various cities, which could be as high as 31% for some of major Iranian cities.