bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drp1 activates ROS/HIF-1α/EZH2 and triggers mitochondrial fragmentation to deteriorate hypercalcemia-associated neuronal injury in mouse model of chronic kidney disease
Authors: Sun Hongming , Li Xitong , Chen Xin , Xiong Yingquan , Cao Yaochen , Wang Ziqiang 
Published: September 2022

Abstract
Background
Chronic kidney disease (CKD), characterized as renal dysfunction, is regarded as a major public health problem which carries a high risk of cardiovascular diseases. The purpose of this study is to evaluate the functional significance of Drp1 in hypercalcemia-associated neuronal damage following CKD and the associated mechanism.

Methods
Initially, the CKD mouse models were established. Next, RT-qPCR and Western blot analysis were performed to measure expression of Fis1 and Drp1 in CKD. Chromatin immunoprecipitation (ChIP) assay and dual-luciferase reporter gene assay were utilized to explore the relationship among Drp1, HIF-1α, EZH2, and ROS with primary cortical neurons isolated from neonatal mice. Next, CKD mice were subjected to calcitonin treatment or manipulation with adenovirus expressing sh-Drp1, so as to explore the effects of Drp1 on hypercalcemia-induced neuronal injury in CKD. TUNEL assay and immunofluorescence staining were performed to detect apoptosis and NeuN-positive cells (neurons) in prefrontal cortical tissues of CKD mice.

Results
It was found that hypercalcemia could induce neuronal injury in CKD mice. An increase of Fis1 and Drp1 expression in cerebral cortex of CKD mice correlated with mitochondrial fragmentation. Calcitonin suppressed Drp1/Fis1-mediated mitochondrial fragmentation to attenuate hypercalcemia-induced neuronal injury after CKD. Additionally, Drp1 could increase EZH2 expression through the binding of HIF-1α to EZH2 promoter via elevating ROS generation. Furthermore, Drp1 knockdown inhibited hypercalcemia-induced neuronal injury in CKD while overexpression of EZH2 could reverse this effect in vivo.

Conclusion
Taken together, the key findings of the current study demonstrate the promotive role of Drp1 in mitochondrial fragmentation which contributes to hypercalcemia-induced neuronal injury in CKD.
Flufenamic acid improves survival and neurologic outcome after successful cardiopulmonary resuscitation in mice
Authors: Chen Jiancong , Chang Yuan , Zhu Juan , Peng Yuqin , Li Zheqi , Zhang Kunxue , Zhang Yuzhen , Lin Chuman , Lin Zhenzhou , Pan Suyue , Huang Kaibin 
Published: September 2022

Abstract
Background
Brain injury is the main cause of high mortality and disability after successful cardiopulmonary resuscitation (CPR) from sudden cardiac arrest (CA). The transient receptor potential M4 (TRPM4) channel is a novel target for ameliorating blood–brain barrier (BBB) disruption and neuroinflammation. Herein, we tested whether flufenamic acid (FFA), which is reported to block TRPM4 with high potency, could confer neuroprotection against brain injury secondary to CA/CPR and whether its action was exerted by blocking the TRPM4 channel.

Methods
Wild-type (WT) and Trpm4 knockout (Trpm4−/−) mice subjected to 10-min CA/CPR were randomized to receive FFA or vehicle once daily. Post-CA/CPR brain injuries including neurologic deficits, survival rate, histological damage, edema formation, BBB destabilization and neuroinflammation were assessed.

Results
In WT mice subjected to CA/CPR, FFA was effective in improving survival and neurologic outcome, reducing neuropathological injuries, attenuating brain edema, lessening the leakage of IgG and Evans blue dye, restoring tight junction protein expression and promoting microglia/macrophages from the pro-inflammatory subtype toward the anti-inflammatory subtype. In comparison to WT mice, Trpm4−/− mice exhibited less neurologic deficiency, milder histological impairment, more BBB integrity and more anti-inflammatory microglia/macrophage polarization. As expected, FFA did not provide a benefit of superposition compared with vehicle in the Trpm4−/− mice after CA/CPR.

Conclusions
FFA mitigates BBB breach and modifies the functional status of microglia/macrophages, thereby improving survival and neurologic deficits following CA/CPR. The neuroprotective effects occur at least partially by interfering with the TRPM4 channel in the neurovascular unit. These results indicate the significant clinical potential of FFA to improve the prognosis for CA victims who are successfully resuscitated.
Effect of ischemic compression on myofascial pain syndrome: a systematic review and meta-analysis
Authors: Lu Wei , Li Jiong , Tian Ye , Lu Xingang 
Published: September 2022

Abstract
Background
Myofascial pain syndrome (MPS) is a condition with local and referred pain characterized by trigger points (taut bands within the muscle). Ischemic compression is a noninvasive manual therapy technique that has been employed for the treatment of MPS in past decades. However, little attention has been devoted to this topic.

Objectives
The present review was designed to explore the efficacy of ischemic compression for myofascial pain syndrome by performing a descriptive systematic review and a meta-analysis to estimate the effect of ischemic compression on MPS.

Methods
A systematic review and meta-analysis concerning randomized controlled trials (RCTs) with myofascial pain subjects who received ischemic compression versus placebo, sham, or usual interventions. Five databases (PubMed, The Cochrane Library, Embase, Web of Science, Ovid) were searched from the earliest data available to 2022.1.2. The standardized mean difference (SMD) and the 95% confidence interval (CI) were used for statistics. Version 2 of the Cochrane risk of tool 2 (RoB 2) was used to assess the quality of the included RCTs.

Results
Seventeen studies were included in the systematic review, and 15 studies were included in the meta-analysis. For the pressure pain threshold (PPT) index, 11 studies and 427 subjects demonstrated statistically significant differences compared with the control at posttreatment (SMD = 0.67, 95% CI [0.35, 0.98], P < 0.0001, I2 = 59%). For visual analog scale (VAS) or numeric rating scale (NRS) indices, 7 studies and 251 subjects demonstrated that there was no significant difference between ischemic compression and controls posttreatment (SMD = − 0.22, 95% CI [− 0.53, 0.09], P = 0.16, I2 = 33%).

Conclusion
Ischemic compression, as a conservative and noninvasive therapy, only enhanced tolerance to pain in MPS subjects compared with inactive control. Furthermore, there was no evidence of benefit for self-reported pain. The number of currently included subjects was relatively small, so the conclusion may be changed by future studies. Big scale RCTs with more subjects will be critical in future.
Robotic-assisted pulmonary lobectomy with lung cancer in a patient with situs inversus totalis
Authors: Yang Chen , Jin Wenjian , Fan Xiao , Zheng Liang , Wang Hui , Wang Qianyun 
Published: September 2022

Abstract
Background
Situs inversus totalis (SIT) is a relatively rare congenital abnormality in which the major thoracic and abdominal visceral organs are reversed from their usual positions. In patients with SIT and bronchial carcinoma, surgical difficulty increases sharply. It has been reported that the video-assisted thoracic surgery (VATS) still poses the operator to a challenge situation. The similarity of surgical positions and the flexibility of the mechanical arm in robotic surgery, may be beneficial to SIT patients due to reducing technical difficulties. Here, we present a first case of SIT patient with lung cancer, in which Da Vinci robot-assisted thoracic surgery (RATS) was performed successfully.

Case presentation
A 66-year old patient, previously diagnosed with SIT since childhood, came to our hospital with two pulmonary nodules in his left lung field. The bigger one had increased somewhat for the last 2 years of follow-up. Software Mimics was preoperatively carried out to analyze anatomical variations. RATS was conducted to complete left upper lobectomy and left middle wedge resection. The patient had no intraoperative complications and was discharged day 5 after the operation.

Conclusions
This is the first report of a successful robot-assisted lung cancer resection in a patient with SIT. In such challenging cases as lung cancer and rare anomaly as SIT, RATS is more advantageous and suitable than VATS with the help of software Mimics utilized for 3D reconstruction, which can identify the anatomical abnormalities and facilitate the surgical procedures.
Integrating spaceborne LiDAR and Sentinel-2 images to estimate forest aboveground biomass in Northern China
Authors: Jiang Fugen , Deng Muli , Tang Jie , Fu Liyong , Sun Hua 
Published: September 2022

Abstract
Background
Fast and accurate forest aboveground biomass (AGB) estimation and mapping is the basic work of forest management and ecosystem dynamic investigation, which is of great significance to evaluate forest quality, resource assessment, and carbon cycle and management. The Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2), as one of the latest launched spaceborne light detection and ranging (LiDAR) sensors, can penetrate the forest canopy and has the potential to obtain accurate forest vertical structure parameters on a large scale. However, the along-track segments of canopy height provided by ICESat-2 cannot be used to obtain comprehensive AGB spatial distribution. To make up for the deficiency of spaceborne LiDAR, the Sentinel-2 images provided by google earth engine (GEE) were used as the medium to integrate with ICESat-2 for continuous AGB mapping in our study. Ensemble learning can summarize the advantages of estimation models and achieve better estimation results. A stacking algorithm consisting of four non-parametric base models which are the backpropagation (BP) neural network, k-nearest neighbor (kNN), support vector machine (SVM), and random forest (RF) was proposed for AGB modeling and estimating in Saihanba forest farm, northern China.

Results
The results show that stacking achieved the best AGB estimation accuracy among the models, with an R2 of 0.71 and a root mean square error (RMSE) of 45.67 Mg/ha. The stacking resulted in the lowest estimation error with the decreases of RMSE by 22.6%, 27.7%, 23.4%, and 19.0% compared with those from the BP, kNN, SVM, and RF, respectively.

Conclusion
Compared with using Sentinel-2 alone, the estimation errors of all models have been significantly reduced after adding the LiDAR variables of ICESat-2 in AGB estimation. The research demonstrated that ICESat-2 has the potential to improve the accuracy of AGB estimation and provides a reference for dynamic forest resources management and monitoring.
Prenatal diagnosis of trisomy 8 mosaicism, initially identified by cffDNA screening
Authors: Hu Junjie , Yan Kai , Jin Pengzhen , Yang Yanmei , Sun Yixi , Dong Minyue 
Published: September 2022

Abstract
Background
So called cell-free fetal DNA (cffDNA) in the maternal plasma, which is derived from placenta, is widely used to screen fetal aneuploidies, including trisomy 21, 18, 13 and sex chromosomes. Here we reported a case of trisomy 8 mosaicism (T8M), which was initially identified via cffDNA screening in noninvasive prenatal testing (NIPT).

Methods
A 35-year-old woman received cffDNA screening at 17th week of gestation. Amniocentesis was performed subsequently, and karyotyping, single-nucleotide polymorphism array (SNP-array) and BACs-on-Beads™ (BoBs™) were used to determine fetal chromosome content. Interphase fluorescence in situ hybridization (FISH) was applied to determine the copy number of chromosome 8.

Results
An enhanced risk for fetal trisomy 8 was identified by cffDNA screening in the studied pregnant woman. After amniocentesis trisomy 8 was found in 1 of 73 metaphases. SNP-array on DNA derived from cultured amniocytes and neonatal cord blood cells suggested the presence of T8M. Interphase FISH on native neonatal cord blood cells confirmed T8M with a percentage of 10%. The Bobs™ fluorescence data also suggested that 8q23-8q24 was amplified.

Conclusions
The current study shows that NIPT is suited to provide hints on rare autosomal trisomies, which have to be further validated and confirmed by other approaches.
Functional performance of the upper limb and the most common boxing-related injuries in male boxers: a retrospective, observational, comparative study with non-boxing population
Authors: Giannatos Vasileios , Panagopoulos Andreas , Antzoulas Panagiotis , I. Giakoumakis Savvas , Lakoumentas John , Kouzelis Antonis 
Published: September 2022

AbstractBackgroundTo investigate the functional status and recording the most common injuries of the upper limb in male Greek boxing squad in comparison to the general population.MethodsA retrospective injury surveillance study using an electronic questionnaire was performed in 2021. The questionnaire was sent to male members of the Greek Boxing Federation and consisted of three parts. Demographic data, functional status scales, training conditions, hours of training, the location and description of upper limb injuries and whether the injury occurred during competition or training and also whether it was a new or a recurrent one were gathered. The same questionnaire was sent to non-boxer males (military recruits), but without asking them to report any training parameters. Inclusion criteria were age < 35 years-old for all participants and no involvement in martial arts for the control group. Also, all participants (boxers and non-boxers) completed the Patient Rated Wrist Evaluation (PRWE) scale and the Quick Disabilities of Arm, Shoulder and Hand (quick-DASH) score.ResultsThe final study cohort was consisted of 62 elite or amateur boxers and 75 non-boxer males, less than 35 years old. The quick-DASH score was found to be significantly lower (better) in boxers in comparison to the general population (15.65 ± 10.25 vs. 12.55 ± 8.62;p = 0.020) whereas the PRWE score was similar in both groups (9.25 ± 14.96 vs. 8.61 ± 13.05;p = 0.843). Physical therapy sessions, thumb injuries and boxer’s knuckle were also found to be significantly higher in the boxers group. On the other hand, upper limb surgeries were significantly less in the boxers group. Finally, the size of boxing gloves was associated to the number of finger fractures, thumb injuries and ulnar sided wrist pain in boxers.ConclusionsAlthough a controversial sport, boxing appears to have no long-term consequences to the upper limb function, especially regarding hand performance. The size of gloves during heavy bag training was found to be an aggravating factor for hand injuries.
E-cadherin maintains the undifferentiated state of mouse spermatogonial progenitor cells via β-catenin
Authors: Song Weixiang , Zhang Danchen , Mi Jiaqi , Du Wenfei , Yang Yang , Chen Rong , Tian Cong , Zhao Xiaodong , Zou Kang 
Published: September 2022

AbstractBackgroundCadherins play a pivotal role in facilitating intercellular interactions between spermatogonial progenitor cells (SPCs) and their surrounding microenvironment. Specifically, E-cadherin serves as a cellular marker of SPCs in many species. Depletion ofE-cadherinin mouse SPCs showed no obvious effect on SPCs homing and spermatogenesis.ResultsHere, we investigated the regulatory role of E-cadherin in regulating SPCs fate. Specific deletion ofE-cadherinin germ cells was shown to promote SPCs differentiation, evidencing by reduced PLZF+population and increasedc-Kit+population in mouse testes.E-cadherinloss down-regulated the expression level ofβ-catenin, leading to the reduced β-catenin in nuclear localization for transcriptional activity. Remarkably, increasing expression level of Cadherin-22 (CDH22) appeared specifically afterE-cadherindeletion, indicating CDH22 played a synergistic effect with E-cadherin in SPCs. By searching for the binding partners of β-catenin, Lymphoid enhancer-binding factor 1 (LEF1), T-cell factor (TCF3), histone deacetylase 4 (HDAC4) and signal transducer and activator 3 (STAT3) were identified as suppressors of SPCs differentiation by regulating acetylation of differentiation genes with PLZF.ConclusionsTwo surface markers of SPCs, E-cadherin and Cadherin-22, synergically maintain the undifferentiation of SPCs via the pivotal intermediate molecule β-catenin. LEF1, TCF3, STAT3 and HDAC4 were identified as co-regulatory factors of β-catenin in regulation of SPC fate. These observations revealed a novel regulatory pattern of cadherins on SPCs fate.
What evidence exists on the impact of sustainability initiatives on smallholder engagement in sustainable palm oil practices in Southeast Asia: a systematic map protocol
Authors: Lai Jia Yen , Mardiyaningsih Dyah Ita , Rahmadian Faris , Hamzah Nurfatin 
Published: September 2022

Abstract
Background
Smallholding plantations represent approximately 40% of the total palm oil plantation area globally. For any certifications, standards, and other instruments to achieve more ethical and sustainable palm oil supply chains, it is essential to improve smallholder engagement in the schemes. A large body of research has built up our understanding of the challenges of engaging smallholders in sustainability initiatives in various sites and countries. A broad systematic understanding of how different types of sustainability initiatives can support or restrict smallholders from access to market and different resources and under which economic and social conditions are not yet developed. This systematic map aims to identify, map, and describe the body of evidence that exists on the positive and negative impacts of sustainability initiatives on smallholder engagement in palm oil practices in Southeast Asia. The findings are expected to inform policies and practices on smallholder engagement in sustainable palm oil supply chains and identify evidence gaps where future primary studies and evidence syntheses can contribute.

Methods
We will develop a guiding framework of interventions through other works on supply chain instruments. We will then construct a test library of 39 items through field expert consultations and snowballing using literature search algorithms. The search will cover four publication databases, five bibliographic databases, and 13 topical and organizational websites. We will search for existing evidence syntheses and primary research studies in Southeast Asia countries published between 2008 and 2021. This systematic map will only include English language articles due to our limited capacity. We will screen the search results at the title/abstract and the full-text levels. Numbers of included/excluded items and reasons for exclusion will be noted and visualized via a ROSES flow diagram. We will develop a data extraction form for assessing data useful for reporting current trends of smallholder engagement in sustainable palm oil initiatives. A random sample of 20% of the included articles will be assessed for validity using Joanne Briggs Institute’s critical appraisal checklist. We will then organize and summarize the data according to the defined PICO.
Application of nonparametric item response theory in determining the one-dimensionality and adaptability of TOEFL iBT listening test
Authors: Ghaemi Hamed 
Published: September 2022

AbstractListening comprehension in English, as one of the most fundamental skills, has an essential role in the process of learning English. Non-parametric item Response Theory (NIRT) is a probabilistic-nonparametric approach to item response theory (IRT) which determines the one-dimensionality and adaptability of test. NIRT techniques are a useful tool for researchers who wish to construct one-dimensional tests. The current study utilized the NIRT to examine adaptability of TEOFL iBT listening test, administered on 400 EFL university students in the Iranian context. The results illustrated no main concerns in terms of item adaptability. It was concluded that the ordering of items according to their mean is invariant across examinees. Dimensionality analysis results depicted that the test is one dimensional -confirming evidence of the validity of the test in measuring a single ability dimension. The empirical application depicted a potential and feasible approach, whereby NIRT could be used as a valuable method for exploring the behavior of scaled items in response to varying levels of a latent trait in education research.
Vitality evaluation of historical and cultural districts based on the values dimension: districts in Beijing City, China
Authors: Zhang Yan , Han Yikuan 
Published: September 2022

Abstract
Historical and cultural districts are an important part of the urban heritage environment, reflecting the urban characteristics and carrying the core value of urban development. At present, most of the research on the vitality of historical and cultural districts mainly carries out quantitative evaluation from the two aspects of material space and time dimension, while the analysis on the value level is weakened, and the content of value type evaluation is also ignored. Value is the main factor affecting the vitality of historical and cultural districts, and it is also the core content to keep them alive. Therefore, this study takes nine historical and cultural districts of three types (cultural, commercial and residential) in Beijing, the capital of China, as examples to form a vitality evaluation framework with value as the evaluation goal, and select three indicators: historical value (including historical environment, cultural environment and spatial environment), use value (including communication environment, business environment and residential environment) and sustainable value (including educational environment, attraction and creativity), which correspond to the past, present and future of the heritage respectively. The literature summary method is used to get the evaluation content, the ranking analytic hierarchy process is used to determine the index weight, experts are invited to score, and the influence degree of each factor is comprehensively ranked and analyzed. Finally, it puts forward the evaluation system of historical and cultural districts. The framework can be used to collect expert opinions, conduct quantitative value evaluation, and adjust various indicators in practice before the protection strategy of historical and cultural districts is proposed. The results show that at present, Shichahai, Nanluoguxiang and Fuchengmennei have the highest vitality among the three types. The blocks that mainly embody cultural value have higher vitality than those that mainly embody commercial value and residential value.
A Perfect Storm Averted: Flawed Systems, a Dropped Ball, and Cognitive Biases Delay a Critical Diagnosis
Authors: Roberts Thomas J. , Sellars Maclean C. , Sands Jacob M. , Jacobson Joseph O. 
Published: September 2022

This is the first case of Cancer Morbidity, Mortality, and Improvement Rounds, a series of articles intended to explore the unique safety risks experienced by oncology patients through the lens of quality improvement, systems and human factors engineering, and cognitive psychology. This case highlights how multiple overlapping factors contributed to a delay in diagnosing disseminated tuberculosis in a patient with lung cancer. The discussion focuses on the ways that cognitive biases contributed to the delayed diagnosis in a patient who, with the benefit of hindsight, exhibited several signs and symptoms suggesting tuberculosis. Cancer Morbidity, Mortality, and Improvement Rounds is a series of articles intended to explore the unique safety risks experienced by oncology patients through the lens of quality improvement, systems and human factors engineering, and cognitive psychology. For purposes of clarity, each case focuses on a single theme, although, as is true for all medical incidents, there are almost always multiple, overlapping, contributing factors. The quality improvement paradigm used here, which focuses on root cause analyses and opportunities to improve care delivery systems, was previously outlined in this journal. 1