bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parameters of the Infrastructure of the Residential Area and Their Relationship with Cardiovascular Risk Factors
Authors: Mulerova T. A. , Gaziev T. F. , Bazdyrev E. D. , Indukaeva E. V. , Tsygankova D. P. , Nakhratova O. V. , Agienko A. S. , Artamonova G. V. 
Published: September 2022

Aim. This study determined the level of public satisfaction with neighborhood design features in Kemerovo Oblast and their connection to cardiovascular disease risk factors.Subjects and methods. The study population included 1,598 respondents aged between 35 and 70, with 491 living in rural areas and others living in Kemerovo (1,221 women and 477 men). The assessment of neighborhood environment was done according to residents' subjective opinions about infrastructural features (the Neighborhood Environmental Walkability Scale). Depending on how participants responded to the questionnaire, some of these parameters were identified as adverse.Results. The residents of Kemerovo and rural areas of Kemerovo Oblast identified the following neighborhood design features as adverse: the lack of interesting places in neighborhood environment, the remote location of parks and restaurants, the absence of pavement, busy traffic, and a long distance between home and workplace. In the city, arterial hypertension prevalence was high among men, when grocery, fruit and clothing stores were distant, and among women, when banks, public transport stops were distant and the traffic was heavy. Lipid metabolism disorders were more common among women in urban areas, when there were no interesting places around. Obesity prevalence was high among urban female population, when the following adverse factors were present: the remote location of grocery stores, fruit stores, bank, pharmacy and public transport stops and the absence of pavement. Among rural male population, this risk factor was common when there were no pavements. The highest rate of carbohydrate metabolism disorders was found among women living in villages where the traffic is heavy and public transport stops are far away.Conclusion. The impact of infrastructure on the health status of the living population is a new direction of scientific research. Epidemiological studies in different geographic areas and population groups show significant differences in health status, morbidity and mortality from chronic noncommunicable diseases. To reduce the risks of developing diseases of the cardiovascular system, the formation of a socially comfortable health-saving environment is of great importance.
Therapeutic Options for the Single Pill Combination of Lisinopril, Amlodipine and Rosuvastatin: a Systematic Review
Authors: Gilyarevsky S. R. , Golshmid M. V. , Bendeliani N. G. 
Published: September 2022

Aim. To evaluate the effect of taking a single pill combination of amlodipine, lisinopril and rosuvastatin on blood pressure (BP) and low-density lipoprotein cholesterol (LDL-C) in hypertensive patients with or without severe hypercholesterolemia.Material and methods. Articles published in Russian were selected for analysis. Six articles that met the criteria for inclusion in a systematic review were found by searching the eLibrary database for the keyword "equamer". The results of 5 observational clinical studies were presented in these articles. The effectiveness of the fixed combination of amlodipine, lisinopril and rosuvastatin was assessed mainly by changes in the level of systolic and diastolic blood pressure, the concentration of LDL cholesterol. In addition, the effects of fixed-dose amlodipine, lisinopril, and rosuvastatin on central aortic pressure and its increment index, as well as carotid-femoral pulse wave velocity, were studied in part of the studies. The effect of the fixed combination of amlodipine, lisinopril and rosuvastatin on blood pressure and LDL-C concentration, as well as on these additional indicators, in patients who had a coronavirus infection with severe lung damage was studied in one study.Results. Evidence from a systematic review demonstrates the efficacy of single pill combination amlodipine, lisinopril and rosuvastatin in reducing blood pressure and LDL-C in a wide range of patients with different baseline risk of developing cardiovascular complications and different baseline levels of blood pressure and LDL-C.Conclusion. The data obtained confirm the feasibility of more frequent prescription of the single pill combination of amlodipine, lisinopril and rosuvastatin in clinical practice for the treatment of hypertensive patients with high or moderate risk of developing cardiovascular diseases, including patients with concomitant hypercholesterolemia.
Shoulder Internal Rotation Contracture Formation in Surgically Managed C5, C6 Brachial Plexus Birth Injuries
Authors: Ulmann Eva T.E. , Malessy Martijn J.A. , Nagels Jochem , Pondaag Willem 
Published: September 2022

Background:
A typical feature in infants with severe C5-C6 brachial plexus birth injury (BPBI) requiring nerve repair is the formation of shoulder internal rotation contracture (IRC). The underlying pathophysiological mechanism is unknown, and the sequelae can be difficult to treat. The severity of the IRC differs among children. C5-C6 lesions are heterogeneous at the root level. Our null hypothesis was that the type of root-level lesion (axonotmesis or neurotmesis versus avulsion) was not associated with the extent of IRC formation over time in children with upper-trunk BPBI.

Methods:
We performed a retrospective analysis of all patients with upper-trunk BPBI who underwent primary surgery of the C5 and/or C6 spinal nerves between 1990 and 2020 and had follow-up of at least 2 years. The primary outcome was passive shoulder external rotation (ER) in adduction at 1, 3, 5, 7, and 15 years of age. The secondary outcome was whether additional shoulder surgery was performed. The relationship between the nature of the C5-C6 lesion and IRC formation was analyzed using linear mixed models. The Kaplan-Meier method was used to estimate the cumulative risk of secondary shoulder procedures.

Results:
In total, 322 patients were analyzed; mean follow-up was 7.2 ± 4.6 years. The C5-C6 root lesion type was significantly related to the passive range of ER (overall test in linear mixed model, p = 0.007). Children with avulsion of C5 and C6 (n = 21) had, on average, 18° (95% confidence interval [CI], 6.3° to 30°) less IRC formation than those with neurotmesis of C5 and C6 (n = 175) and 17° (2.9° to 31°) less than those with neurotmesis of C5 and avulsion of C6 (n = 34). IRC formation did not differ between the neurotmesis C5-C6 and neurotmesis C5-avulsion C6 groups. Secondary shoulder procedures were performed in 77 patients (10-year risk, 28% [95%CI, 23% to 34%]).

Conclusions:
Shoulder IRC formation in infants with BPBI with surgically treated C5-C6 lesions occurs to a lesser degree if the C5 root is avulsed than when C5 is neurotmetic. This finding provides insight into the possible causative pathoanatomy and may ultimately lead to strategies to mitigate IRC.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
Long non-coding RNA SREBF2-AS1 promotes cell progression by increasing SREBF2 expression in Hepatocellular carcinoma
Authors: Su Chuan , Qu Lili , Cai Xiaoxiao , Shen Peng , Zhu Yefei 
Published: September 2022

Abstract
Objective: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. Long non-coding RNAs (lncRNAs) are emerging as one of important regulators that may be involved in the progression of cancers in humans.
Methods:Comprehensive analysis of the lncRNA expression profile of HCC was performed by using TCGA and Gene Expression Omnibus (GEO) database to screen the target lncRNA(s). LncRNA of SREBF2-AS1 was selected and its expression level in a cohort of 15 pairs of HCC tissues was verified by quantitative real-time PCR (qRT-PCR). Loss-of-function and gain-of-function assays were carried out to investigate the role of SREBF2-AS1 in HCC progression in vitro. Tumor formation assay was performed to verity the role of SREBF2-AS1 in HCC progression in vivo.
Results: Database analysis showed that the expression of SREBF2-AS1 was upregulated in HCC, which was correlated with neoplasm grade and over survival time. The expression of SREBF2-AS1 was verified in a cohort of 15 pairs of HCC tissues. SREBF2-AS1 knockdown mitigated HCC cell growth and promoted apoptosis in vitro and in vivo. Whereas, SREBF2-AS1 overexpression promoted tumor cell growth. Furthermore, our investigation demonstrated that the oncogenic activity of SREBF2-AS1 is partially attributable to the regulation of sterol regulatory element-binding protein 2 (SREBF2) expression.
Conclusions: Our study highlights the regulatory role of SREBF2-AS1 in promoting HCC progression, suggesting that SREBF2-AS1 might be a potent therapeutic target by regulating the expression of SREBF2 for patients with HCC.
Efficient Load Balancing in Cloud Environment Using Improved Spider Monkey Optimization
Authors: Verma Garima 
Published: September 2022

Abstract
Cloud computing provides a shared resource pool in a distributed environment. Users can access these resources anywhere anytime as per the provider’s policy. Whenever tasks are submitted on the cloud for execution, they needs to be scheduled appropriately in the cloud environment as many Virtual Machines (VM) are available at the backend. The performance of the entire system depends on the scheduling algorithm. A good scheduling algorithm distributes given tasks among all VMs so that the load of each VM is balanced. This problem is called a load balancing problem and comes under the category of NP-Hard problems. This paper uses the Spider Monkey Optimization algorithm for more efficient load balancing. The proposed optimization algorithm aims to enhance the performance by selecting the minimum loaded VM for balancing. Results of simulation show that proposed algorithm performance is improved compared to the existing algorithms with respect to load balancing, response time, make span, and resource utilization.
Assessing the Potential Chronic, Lethal, and Multigenerational Ecotoxicity of Land-Applying Biosolids using Zea mays, Glycine max, Phaseolus vulgaris and Brassica rapa
Authors: Puddephatt Karen Joan , McCarthy Lynda Helen , Serre Bryant Micheal 
Published: September 2022

Abstract
The uncertainty of potential toxicity when land-applying municipal biosolids to agricultural fields needs to be clarified considering the concomitant benefit for nutrient amendment and sustainability of resource recovery. This research is part of a larger program that assessed the toxicity of biosolids to terrestrial and aquatic organisms and this study specifically examined the toxicity of two biosolids when applied to four environmentally-relevant field crops. New bioassays were necessary to test the ecotoxicity of biosolids throughout the entire life cycle of each crop: Zea mays (corn), Glycine max (soybeans), Phaseolus vulgaris (common bean), and Brassica rapa (field mustard). It was hypothesized that biosolids would exhibit impact at both an environmentally-relevant application rate (8 tonnes ha-1) and a worst-case scenario (22 tonnes ha-1). The ecotoxicity of biosolids was tested using chronic, lethal, and multigenerational endpoints (i.e., F1 generation viability). Overall, study findings indicated a positive response to nutrient amendment using biosolids at either application rate. Negative responses to biosolids were seen in early growth stages of some cultivars (Zea mays) but disappeared or became positive as plants matured: these observations would have been made if existing protocols had been followed. Brassica rapa exhibited a negative germination rate when exposed to biosolids; however, further work is necessary to elucidate whether the effect is a result of nutrient additions or physical compaction on the small seed. The complete life-cycle bioassays of crops suggest that plants grown in the biosolids-amended soil were significantly larger and produced more seeds compared to reference assays. These results lend scientific support for their sustainable use in land-application strategies in Canada.
Is area-level socioeconomic deprivation associated with mortality due to circulatory system diseases in Poland?
Authors: Jamiołkowski Jacek , Genowska Agnieszka , Pająk Andrzej 
Published: September 2022

Abstract
Background.
Socioeconomic deprivation (SED) is known to influence cardiovascular health. However, studies analyzing the relationship between deprivation and circulatory system diseases (CSD) in Central and Eastern Europe are limited. This study aimed to assess the relationship between SED and mortality due to CSD at a population level in 66 sub-regions of Poland.
Methods.
The data regarding mortality and SED components were obtained from the Central Statistical Office. An area-based SED index was estimated from the rates of education, structure in employment, salary, unemployment, as well as and poverty. The dynamics of changes in mortality due to CSD was calculated as the number of deaths prevented or postponed (DPP) in terciles of SED index. The associations between the mortality from CSD and SED index were analyzed using multivariate Poisson regression models and generalized estimating equations.
Results.
Among men, the percentage of DPP in 2014 was 13.1% for CSD, 23.4% for ischemic heart disease (IHD), and 21.4% for cerebrovascular diseases (CD). In the case of women, the proportion of DPP was 12.8%, 25.6%, and 21.6%, respectively. More deprived sub-regions experienced a greater decrease in CSD-related mortality compared to the less deprived sub-regions. The disparity in mortality reduction between more deprived and less deprived sub-regions was even more pronounced for women. After adjustment for lifestyle, population density, and changes in mortality over time, the SED index was found to be associated with CSD- and IHD-related mortality for men (β = 1.053, 95% confidence interval (CI) 1.008–1.100 and β = 1.195, 95% CI 1.005–1.419, respectively), and with IHD- and CD-related mortality for women (β = 1.303, 95% CI 1.025–1.657 and β = 1.230, 95% CI 1.025–1.477, respectively).
Conclusions.
A major proportion of the territorial differences in mortality due to CSD in Poland could be attributed to socioeconomic deprivation. To reduce CSD mortality, more comprehensive preventive measures, including approaches addressing the socioeconomic factors, mainly poverty, education and employment, in particular in less urbanized areas are needed.
Molecular characterisation and tracking of the severe acute respiratory syndrome coronavirus 2, Thailand, 2020–2022
Authors: Puenpa Jiratchaya , Rattanakomol Patthaya , Saengdao Nutsada , Chansaenroj Jira , Yorsaeng Ritthideach , Suwannakarn Kamol , Thanasitthichai Somchai , Vongpunsawad Sompong , Poovorawan Yong 
Published: September 2022

Abstract
The global COVID-19 pandemic caused by a novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first detected in China in December 2019. To date, there have been approximately 3.4 million reported cases and over 24,000 deaths in Thailand. This study investigated the molecular characterisation and the evolution of the SARS-CoV-2 identified during 2020–2022 in Thailand. Two hundred and sixty-eight SARS-CoV-2 strains, collected mostly in Bangkok from COVID-19 patients, were characterised by partial genome sequencing. Moreover, 5,627 positive SARS-CoV-2 samples were identified as viral variants [B.1.1.7 (Alpha), B.1.617.2 (Delta), B.1.1.529 (Omicron/BA.1) and B.1.1.529 (Omicron/BA.2)] by multiplex real-time reverse-transcription polymerase chain reaction (RT-PCR) assays. The results revealed that B.1.36.16 caused the predominant outbreak in the second wave (December 2020–January 2021), B.1.1.7 (Alpha) in the third wave (April–June 2021), B.1.617.2 (Delta) in the fourth wave (July–December 2021), and B.1.1.529 (Omicron) in the fifth wave (January–March 2022). The evolutionary rate of the viral genome was 2.60×10− 3 (95% highest posterior density [HPD], 1.72×10− 3 to 3.62×10− 3) nucleotide substitutions per site per year. Continued molecular surveillance of SARS-CoV-2 is crucial for monitoring emerging variants with the potential to cause new COVID-19 outbreaks.
Steering to intercept targets following curving trajectories: Insights into visual guidance from Qualitative Inconsistency Detection
Authors: Opstal A. A. M. (Daphne) Van , Casanova Remy , Zaal Frank T. J. M. , Bootsma Reinoud J. 
Published: September 2022

Abstract
This study explored the informational variables guiding steering behaviour in a locomotor interception task with targets moving along circular trajectories. Using a new method of analysis focussing on the temporal co-evolution of steering behaviour and the potential information sources driving it, we set out to invalidate reliance on plausible informational candidates. Applied to individual trials rather than ensemble averages, this Qualitative Inconsistency Detection (QuID) method revealed that steering behaviour was not compatible with reliance on information grounded in any type of change in the agent-centred target-heading angle. First-order changes in the environment-centred target’s bearing angle could also not adequately account for the variations in behaviour observed under the different experimental conditions. Capturing the observed timing of unfolding steering behaviour ultimately required a combination of (velocity-based) first-order and (acceleration-based) second-order changes in bearing angle. While this result may point to reliance on fractional-order based changes in bearing angle, the overall importance of the present findings resides in the demonstration of the necessity to break away from the existing practice of trying to fit behaviour into a priori postulated functional strategies based on categorical differences between operative heuristic rules or control laws.
Seroprevalence of zoonotic abortive diseases and their associated risk factors in Tunisian sheep
Authors: Guesmi Kaouther , Kalthoum Sana , Mamlouk Aymen , Baccar Mohamed Naceur , Mohamed Bassem Bel Haj , Hajlaoui Haikel , Cherni Jamel , Seghaier Chédia , Messadi Lilia , Toumi Aymen 
Published: September 2022

Abstract
Background Abortion is a serious problem for sheep flocks and it is responsible for considerable economic losses. The epidemiological situation of abortion causing agents in sheep is poorly documented in Tunisia. This study aims to investigate the status of three abortion causing agents (Brucella melitensis, Toxoplasma gondii, and Coxiella burnetii) among organized flocks in Tunisia. ResultsA total of 793 sample sera collected from twenty-six flocks in seven governorates in Tunisia, were tested by indirect enzyme-linked immunosorbent assay (i-ELISA) for antibodies against three abortion causing agents (Brucella melitensis, Toxoplasma gondii, and Coxiella burnetii). Risk factors for individual-level seroprevalence were analyzed using a logistic regression model. Results revealed that 19.7%, 17.2%, and 16.1% of the tested sera were positive for toxoplasmosis, Q fever, and brucellosis, respectively. Mixed infection was found in Logistic regression showed that the management practices (control of new introduction, common grazing and watering point, workers exchange, presence of lambing box on the farm) and the history of infertility and the presence of abortion in neighboring flocks were likely to increase the probability of being infected by the three abortive diseases. ConclusionsEvidence of the positive relationship between seroprevalence of abortion causing agents and several risk factors, suggests further investigations to better understand the etiology of infectious abortions in flocks to develop an applicable preventive and control program.
Probable human origin of the SARS-CoV-2 polybasic furin cleavage site
Authors: Romeu Antonio R. 
Published: September 2022

Abstract
Background: the origin of the current SARS-CoV-2 virus is still elusive. However, the finding that Laos bats host closest known relatives of virus behind Covid that even without the furin site they can infect human cells, it is a giant stride forward to known the progenitor of the virus. The mystery focus on the CGG-CGG code of the SARS-CoV-2 furin site arginine dimer. This genetic footprint has not been observed in natural coronaviruses.
Results: based in a bioinformatic approach I found that the two possible 12-nucleotide fragment (and they reverse complement) properly inserted within the codon S680 in the S gene of the closest relative to the virus, encoding the PRRA furin site motif, 100% match to severe human NCBI RefSeq curated mRNA protein-coding transcripts. The genes to which these transcripts belong are ubiquitously and highly expressed genes, e.g., the alpha subunit of the mitochondrial ATP synthase F1 (ATP5F1) and/or the ubiquitin specific peptidase 21 (USP21); or specific and highly expressed genes in tissues such as small intestine, duodenum, brain, kidney and gonads which are the gateway of the SARS-CoV-2 infection. On the other hand, a significant group of virus specimens have been discovered in which the arginine dimer of the furin site is codon-optimized by the SARS-CoV-2 codon usage bias. Another main finding was to discover other PRRA-like insertions in the S protein from some SARS-CoV-2 specimens.
Conclusions: the first conclusion of this work is that the SARS-CoV-2 is deleting the non-viral origin of the furin site CGG-CGG arginine pair through codon optimization. Based on the results shown here together with the new Laos coronaviruses the main conclusion of this work is that a genetic recombination of unrelated RNA sequences between the a closest relative to SARS-CoV-2 and human transcripts, during an undetected viral circulation in humans, could be the origin of the polybasic furin cleavage site.
Geospatial Analysis of Gully Erosion Causative Factors: A Case Study of Anambra Erosion Prone Site, Southeastern Nigeria
Authors: Emeh Chukwuebuka , Igwe Ogbonnaya , Ugwoke Tochukwu A.S. 
Published: September 2022

Abstract
Gully erosion studies are usually complex and expensive due to the multiple nature of the causative factors, heterogeneity of the underlying geologic materials, and the high volume of point source data required within a given area. For this reason, thorough gully erosion studies are rarely carried out especially in developing countries with little resources allocated to environmental studies. Thus, it becomes difficult in solving problems arising from such geologic hazard in those areas. However, the availability of data emanating from remotely sensed operations can be utilized in solving complex gully erosional problems using modern geospatial analytical tools. Consequently, gully erosion studies within the study area were carried out by integration of geomorphologic and environmental data which were acquired remotely, and geotechnical information derived from field and laboratory investigations of the underlying geologic materials. The integrated geomorphologic, environmental, and geotechnical data was analysed with analytical tools such as ArcGIS, Google Earth, and Microsoft Excel, following the frequency ratio method. Results from the study revealed that slope angle, soil plasticity, angle of internal friction, cohesion, and population density contributed about 20%, 23%, 20%, 18%, and 9%, respectively to soil’s susceptibility to gullying. Slope angle and population density were positively correlated with the frequency of gully erosion, whereas plasticity, cohesion, and angle of internal friction were negatively correlated with frequency of gully erosion. The spatial distribution of the data revealed areas that are susceptible to gullying in their various degrees; thus providing affordable information for proper environmental planning and development.