bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aldehyde oxidases mediate plant toxicant susceptibility and fecundity in the red flour beetle, Tribolium castaneum
Authors: Zhang Yonglei , Zhang Jiahao , Li Dongyu , Sun Haidi , Lu Ruixue , Yin Se , Guo Xinlong , Gao Shanshan 
Published: February 2022

AbstractAldehyde oxidases (AOXs) are a group of metabolic enzymes that play critical roles in the degradation of xenobiotics and chemicals. However, the physiological function of this enzyme in insects remains poorly understood. In this study, three TcAOX genes (TcAOX1, TcAOX2, TcAOX3) were identified and characterized from Tribolium castaneum genome. Spatiotemporal expression profiling showed that TcAOX1 expression was most highly expressed at the early pupal stage and was predominantly expressed in the antennae of adults, indicating that TcAOX1 was involved in the degradation of chemical signals; TcAOX2 expression was most highly expressed at the late pupal stage and was mainly expressed in the fat body, epidermis of larvae and adults, respectively; and TcAOX3 expression was in all stages and was primarily expressed in the head of adults. Moreover, the transcripts of TcAOX2 and TcAOX3 were significantly induced after exposure to plant oil, and RNA interference (RNAi) targeting of each of them enhanced the susceptibility of beetles to this plant toxicant, suggesting that these two genes are associated with plant toxicant detoxification. Intriguingly, knockdown of the TcAOX1 led to reductions in female egg-laying but unchanged the hatchability and the development of genital organs, suggesting that this gene may mediate fecundity by effecting the inactivation of chemical signals in T. castaneum. Overall, these results shed new light on the function of AOX genes in insects, and could facilitate the development of research on pest control management.
A revision of theRostania occultata(Collemataceae) complex in Fennoscandia
Authors: Košuthová Alica , Westberg Martin , Wedin Mats 
Published: February 2022

AbstractTheRostania occultataspecies complex (‘Collema occultatums. lat.’) is revised in Fennoscandia and found to consist of four species, all epiphytes on deciduous trees:Rostania effusaA. Košuth., M. Westb. & Wedin sp. nov.,R. occultata(Bagl.) Otáloraet al.,R. pallidaA. Košuth., M. Westb. & Wedin sp. nov. andR. populina(Th. Fr.) A. Košuth., M. Westb. & Wedin comb. nov.Rostania effusaandR. pallidaare newly described from humid habitats in old-growth boreal coniferous forests, usually with a mixture of deciduous trees, and from similar areas in the subalpine birch-dominated forests of Fennoscandia.Rostania effusais characterized by apothecia with red-brown apothecium discs and an excipulum thallinum with a simple pseudocortex and cubic to oblong, muriform spores.Rostania pallidahas apothecia with whitish to pale yellowish discs and an excipulum thallinum with a distinct cellular pseudocortex, and ellipsoid, muriform mature spores that are often constricted at the centre. A lectotype is designated forCollema quadratumJ. Lahm ex Körb. The new combinationRostania populinais introduced for the species recognized until now as the varietyRostania occultatavar.populina(Th. Fr.) Perlmutter & Rivas Plata. A key to the six species inRostanias. str. is included.
Haemodynamic impact of chest compressions in the delivery room
Authors: Natarajan Girija , Bajaj Monika , Aggarwal Sanjeev 
Published: February 2022

AbstractAmong 65 neonates with encephalopathy undergoing cooling, 30 (46.1%) received chest compressions during delivery room resuscitation. Despite differences in encephalopathy severity, early (<24 hours) biventricular function on echocardiogram (fractional area change, myocardial performance indices, systolic to diastolic duration ratios, tricuspid annular plane systolic excursion) was comparable between groups with and without chest compressions. Epinephrine receipt was associated with abnormal tricuspid annular plane systolic excursion.
The Impact of Trade Barrier Reductions on Global Value Chains for Agricultural Products in China and Countries along the ‘Belt and Road’
Authors: Zhang Zhen , Gao Yue , Wei Taoyuan 
Published: February 2022

AbstractThe Belt and Road Initiative (BRI) may potentially reduce trade barriers between China and countries along the Belt and Road (BR) route, affecting the positions in Global Value Chains (GVCs) of agricultural products of these countries. This study explores the BRI influence on the GVC positions using a global computable general equilibrium (CGE) model and focusing on China and the BR countries. The study finds that the reduction of tariff barriers and non-tariff barriers between countries along ‘the Belt and Road’ results in increased producer prices and volumes of almost all agricultural products exported from China, which improves the position of China in the GVCs of these agricultural products in our best estimate scenario. Countries along the BR route also benefit from the reduction in trade barriers, with improved positions in the GVCs of agricultural products in the best estimate scenario, especially those products that have comparative advantages in GVCs.
Secondary 12‐Day Planetary Wave in the Mesospheric Water Vapor During the 2016/2017 Unusual Canadian Stratospheric Warming
Authors: Qin Yusong , Gu Sheng‐Yang , Dou Xiankang , Teng Chen‐Ke‐Min , Yang Zhenlin 
Published: February 2022

AbstractThe anomalous strengthening of the stratospheric Aleutian High during the early boreal winter of 2016/2017 led to a rare Canadian stratospheric warming (CSW) event, during which the extent of the wind reversal in the Arctic region is the strongest among CSWs since 2001. Unlike sudden stratospheric warmings, the polar vortex during this CSW is merely distorted, not displaced off the pole or split into two pieces, and the polar mean temperature only has a modest increase of a few kelvins. Using the Aura satellite Microwave Limb Sounder measurements, an exceptionally strong 12‐day wave (12DW) with westward zonal wavenumber 1 is captured in the mesospheric water vapor mixing ratio during the recovery phase of this CSW. Further diagnostic analysis suggests that the baroclinic/barotropic instability related to this CSW triggers the 12DW in the Arctic stratosphere, and meridional advection plays a leading role in the modulation of 12DW on mesospheric water vapor.
Bidecadal Temperature Anomalies Over the Tibetan Plateau and Arctic in Response to the 1450s Volcanic Eruptions
Authors: Liu Wei , Shi Feng , Xiao Guoqiao , Xue Huihong , Yin Qiuzhen , Liu Fei , Duan Anmin , Xiao Cunde , Guo Zhengtang 
Published: February 2022

AbstractVolcanic eruptions have been the most dominant natural forcing of climate change over the past millennium, affecting temperature change at multiple timescales. However, how volcanic eruptions affect regional temperature variability on the decadal timescale remains unclear. We analyzed the bidecadal effects of volcanic eruptions in the mid‐fifteenth century on two representative regions (the Tibetan plateau [TP] and the Arctic) in the Northern Hemisphere by combining proxy reconstructions, model ensemble simulations, and model sensitive experiments. The results show that the TP experienced bidecadal cooling during the mid‐fifteenth century as a result of the long‐term heat uptake in the midlatitude ocean that reduced latent heat transfer to the atmosphere. A positive sea ice–albedo feedback led to a bidecadal summer temperature decrease in the Arctic comparable to that on the TP, whereas the Arctic winter experienced stronger bidecadal cooling caused by a reduced ocean–atmosphere energy exchange and atmospheric poleward energy transport. In addition, our results show that it took ∼20 years for the temperature in these two regions to return to the level that existed before the first eruption, and this was linked to the strengthening of the Atlantic Meridional Overturning Circulation and ocean heat transport.
Unraveling the Contribution of Turbulence and Bubbles to Air‐Water Gas Exchange in Running Waters
Authors: Klaus M. , Labasque T. , Botter G. , Durighetto N. , Schelker J. 
Published: February 2022

AbstractQuantifying air‐water gas exchange is critical for estimating greenhouse gas fluxes and metabolism in aquatic ecosystems. In high‐energy streams, the gas exchange rate k is poorly constrained, due to an incomplete understanding of turbulence and bubble contributions to k. We performed a flume experiment with air bubble additions to evaluate the combined effects of turbulence and bubbles on k for helium, argon, xenon, and methane. We created contrasting hydraulic conditions by varying channel slope, bed roughness, water discharge, and bubble flux. We found that k increased from 1–4 to 17–66 m d−1 with increases in turbulence and bubble flux metrics. Mechanistic models that explicitly account for these metrics, as well as gas diffusivity and solubility, agreed well with the data and indicated that bubble‐mediated gas exchange accounted for 64–93% of k. Bubble contributions increased with bubble flux but were independent of gas type, as bubbles did not equilibrate with the water. This was evident through modeled bubble life and equilibration times inferred from bubble size distributions obtained from underwater sound spectra. Sound spectral properties correlated well with turbulence and bubble flux metrics. Our results demonstrate that (a) mechanistic models can be applied to separate free surface‐ and bubble‐mediated gas exchange in running waters, (b) bubble life and equilibration times are critical for accurate scaling of k between different gases, and (c) ambient sound spectra can be used to approximate contributions of turbulence and bubbles.
Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma
Authors: Zhang Panpan , He Qiuping , Wang Yaqin , Zhou Guanqun , Chen Yupei , Tang Linglong , Zhang Yuan , Hong Xiaohong , Mao Yanping , He Qingmei , Yang Xiaojing , Liu Na , Ma Jun 
Published: February 2022

AbstractMetastasis and recurrence account for 95% of deaths from nasopharyngeal carcinoma (NPC). Cancer stem cells (CSCs) are regarded as one of the main reasons for tumor cell resistance to clinical therapy, and cancer metastasis or recurrence, while little is known about CSCs in NPC. The present study uncovers a subpopulation of cells labeled as CD45−EPCAM+PROCR+ in NPC biopsy samples that exhibit stem cell-like characteristics. A relatively low number of these cells initiate xenograft tumors in mice. Functional analysis reveals that protein C receptor (PROCR) not only serves as a stem cell marker in NPC, but also maintains tumor cells’ stemness potential through regulating lipid metabolism and mitochondrial fission. Epistatic studies reveal that cAMP-protein kinase A stimulates Ca2+ release to manipulate lipid metabolism related genes’ expression. Finally, in a cohort of 207 NPC samples, PROCR expression is correlated with tumor metastasis or recurrence, and predicts poor prognosis. These novel findings link PROCR labeled CSCs with lipid metabolism and mitochondrial plasticity, and provides new clinical target against metastatic or recurrent NPC.
Active anaerobic methane oxidation and sulfur disproportionation in the deep terrestrial subsurface
Authors: Bell Emma , Lamminmäki Tiina , Alneberg Johannes , Qian Chen , Xiong Weili , Hettich Robert L , Frutschi Manon , Bernier-Latmani Rizlan 
Published: February 2022

Abstract
Microbial life is widespread in the terrestrial subsurface and present down to several kilometers depth, but the energy sources that fuel metabolism in deep oligotrophic and anoxic environments remain unclear. In the deep crystalline bedrock of the Fennoscandian Shield at Olkiluoto, Finland, opposing gradients of abiotic methane and ancient seawater-derived sulfate create a terrestrial sulfate-methane transition zone (SMTZ). We used chemical and isotopic data coupled to genome-resolved metaproteogenomics to demonstrate active life and, for the first time, provide direct evidence of active anaerobic oxidation of methane (AOM) in a deep terrestrial bedrock. Proteins from Methanoperedens (formerly ANME-2d) are readily identifiable despite the low abundance (≤1%) of this genus and confirm the occurrence of AOM. This finding is supported by 13C-depleted dissolved inorganic carbon. Proteins from Desulfocapsaceae and Desulfurivibrionaceae, in addition to 34S-enriched sulfate, suggest that these organisms use inorganic sulfur compounds as both electron donor and acceptor. Zerovalent sulfur in the groundwater may derive from abiotic rock interactions, or from a non-obligate syntrophy with Methanoperedens, potentially linking methane and sulfur cycles in Olkiluoto groundwater. Finally, putative episymbionts from the candidate phyla radiation (CPR) and DPANN archaea represented a significant diversity in the groundwater (26/84 genomes) with roles in sulfur and carbon cycling. Our results highlight AOM and sulfur disproportionation as active metabolisms and show that methane and sulfur fuel microbial activity in the deep terrestrial subsurface.
Long non-coding ROR promotes the progression of papillary thyroid carcinoma through regulation of the TESC/ALDH1A1/TUBB3/PTEN axis
Authors: Fan Yuxia , Fan Xin , Yan Hao , Liu Zheng , Wang Xiaoming , Yuan Qingling , Xie Jie , Lu Xiubo , Yang Yang 
Published: February 2022

AbstractPapillary thyroidal carcinoma (PTC) is a common endocrine cancer that plagues people across the world. The potential roles of long non-coding RNAs (lncRNAs) in PTC have gained increasing attention. In this study, we aimed to explore whether lncRNA ROR affects the progression of PTC, with the involvement of tescalcin (TESC)/aldehyde dehydrogenase isoform 1A1 (ALDH1A1)/βIII-tubulin (TUBB3)/tensin homolog (PTEN) axis. PTC tumor and adjacent tissues were obtained, followed by measurement of lncRNA ROR and TESC, ALDH1A1, and TUBB3 expression. Interactions among lncRNA ROR, TESC, ALDH1A1, TUBB3, and PTEN were evaluated by ChIP assay, RT-qPCR, or western blot analysis. After ectopic expression and depletion experiments in PTC cells, MTT and colony formation assay, Transwell assay, and flow cytometry were performed to detect cell viability and colony formation, cell migration and invasion, and apoptosis, respectively. In addition, xenograft in nude mice was performed to test the effects of lncRNA ROR and PTEN on tumor growth in PTC in vivo. LncRNA ROR, TESC, ALDH1A1, and TUBB3 were highly expressed in PTC tissues and cells. Overexpression of lncRNA ROR activated TESC by inhibiting the G9a recruitment on the promoter of TESC and histone H3-lysine 9me methylation. Moreover, TESC upregulated ALDH1A1 expression to increase TUBB3 expression, which then reduced PTEN expression. Overexpression of lncRNA ROR, TESC, ALDH1A1 or TUBB3 and silencing of PTEN promoted PTC cell viability, colony formation, migration, and invasion while suppressing apoptosis. Moreover, overexpression of lncRNA ROR increased tumor growth by inhibiting PTEN in vivo. Taken together, the current study demonstrated that lncRNA ROR mediated TESC/ALDH1A1/TUBB3/PTEN axis, thereby facilitating the development of PTC.
CHCHD2 and CHCHD10 regulate mitochondrial dynamics and integrated stress response
Authors: Ruan Yu , Hu Jiaqiao , Che Yaping , Liu Yanyan , Luo Zhenhuan , Cheng Jin , Han Qi , He He , Zhou Qinghua 
Published: February 2022

AbstractMitochondrial dysfunction is becoming one of the main pathology factors involved in the etiology of neurological disorders. Recently, mutations of the coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2) and 10 (CHCHD10) which encode two homologous proteins that belong to the mitochondrial CHCH domain protein family, are linked to Parkinson’s disease and amyotrophic lateral sclerosis (ALS)/frontotemporal dementia (FTD), respectively. However, the physiological and pathological roles of these twin proteins have not been well elaborated. Here, we show that, in physiological conditions, CHCHD2 and CHCHD10 interact with OMA1 and suppress its enzyme activity, which not only restrains the initiation of the mitochondrial integrated response stress (mtISR), but also suppresses the processing of OPA1 for mitochondrial fusion. Further, during mitochondria stress-induced by carbonyl cyanide m-chlorophenylhydrazone (CCCP) treatment, CHCHD2 and CHCHD10 translocate to the cytosol and interacte with eIF2a, which attenuates mtISR overactivation by suppressing eIF2a phosphorylation and its downstream response. As such, knockdown of CHCHD2 and CHCHD10 triggers mitochondrial ISR, and such cellular response is enhanced by CCCP treatment. Therefore, our findings demonstrate the first “mtISR suppressor” localized in mitochondria for regulating stress responses in mammalian cells, which has a profound pathological impact on the CHCH2/CHCH10-linked neurodegenerative disorder.
An efficient urine peptidomics workflow identifies chemically defined dietary gluten peptides from patients with celiac disease
Authors: Palanski Brad A. , Weng Nielson , Zhang Lichao , Hilmer Andrew J. , Fall Lalla A. , Swaminathan Kavya , Jabri Bana , Sousa Carolina , Fernandez-Becker Nielsen Q. , Khosla Chaitan , Elias Joshua E. 
Published: February 2022

AbstractCeliac disease (CeD) is an autoimmune disorder induced by consuming gluten proteins from wheat, barley, and rye. Glutens resist gastrointestinal proteolysis, resulting in peptides that elicit inflammation in patients with CeD. Despite well-established connections between glutens and CeD, chemically defined, bioavailable peptides produced from dietary proteins have never been identified from humans in an unbiased manner. This is largely attributable to technical challenges, impeding our knowledge of potentially diverse peptide species that encounter the immune system. Here, we develop a liquid chromatographic-mass spectrometric workflow for untargeted sequence analysis of the urinary peptidome. We detect over 600 distinct dietary peptides, of which ~35% have a CeD-relevant T cell epitope and ~5% are known to stimulate innate immune responses. Remarkably, gluten peptides from patients with CeD qualitatively and quantitatively differ from controls. Our results provide a new foundation for understanding gluten immunogenicity, improving CeD management, and characterizing the dietary and urinary peptidomes.
Dual clathrin and integrin signaling systems regulate growth factor receptor activation
Authors: Alfonzo-Méndez Marco A. , Sochacki Kem A. , Strub Marie-Paule , Taraska Justin W. 
Published: February 2022

AbstractThe crosstalk between growth factor and adhesion receptors is key for cell growth and migration. In pathological settings, these receptors are drivers of cancer. Yet, how growth and adhesion signals are spatially organized and integrated is poorly understood. Here we use quantitative fluorescence and electron microscopy to reveal a mechanism where flat clathrin lattices partition and activate growth factor signals via a coordinated response that involves crosstalk between epidermal growth factor receptor (EGFR) and the adhesion receptor β5-integrin. We show that ligand-activated EGFR, Grb2, Src, and β5-integrin are captured by clathrin coated-structures at the plasma membrane. Clathrin structures dramatically grow in response to EGF into large flat plaques and provide a signaling platform that link EGFR and β5-integrin through Src-mediated phosphorylation. Disrupting this EGFR/Src/β5-integrin axis prevents both clathrin plaque growth and dampens receptor signaling. Our study reveals a reciprocal regulation between clathrin lattices and two different receptor systems to coordinate and enhance signaling. These findings have broad implications for the regulation of growth factor signaling, adhesion, and endocytosis.
Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription
Authors: Arragain Benoît , Durieux Trouilleton Quentin , Baudin Florence , Provaznik Jan , Azevedo Nayara , Cusack Stephen , Schoehn Guy , Malet Hélène 
Published: February 2022

AbstractSegmented negative-strand RNA bunyaviruses encode a multi-functional polymerase that performs genome replication and transcription. Here, we establish conditions for in vitro activity of La Crosse virus polymerase and visualize its conformational dynamics by cryo-electron microscopy, unveiling the precise molecular mechanics underlying its essential activities. We find that replication initiation is coupled to distal duplex promoter formation, endonuclease movement, prime-and-realign loop extension and closure of the polymerase core that direct the template towards the active site. Transcription initiation depends on C-terminal region closure and endonuclease movements that prompt primer cleavage prior to primer entry in the active site. Product realignment after priming, observed in replication and transcription, is triggered by the prime-and-realign loop. Switch to elongation results in polymerase reorganization and core region opening to facilitate template-product duplex formation in the active site cavity. The uncovered detailed mechanics should be helpful for the future design of antivirals counteracting bunyaviral life threatening pathogens.
Differential V2-directed antibody responses in non-human primates infected with SHIVs or immunized with diverse HIV vaccines
Authors: Weiss Svenja , Itri Vincenza , Pan Ruimin , Jiang Xunqing , Luo Christina C. , Morris Lynn , Malherbe Delphine C. , Barnette Philip , Alexander Jeff , Kong Xiang-Peng , Haigwood Nancy L. , Hessell Ann J. , Duerr Ralf , Zolla-Pazner Susan 
Published: February 2022

AbstractV2p and V2i antibodies (Abs) that are specific for epitopes in the V1V2 region of the HIV gp120 envelope (Env) do not effectively neutralize HIV but mediate Fc-dependent anti-viral activities that have been correlated with protection from, or control of HIV, SIV and SHIV infections. Here, we describe a novel molecular toolbox that allows the discrimination of antigenically and functionally distinct polyclonal V2 Ab responses. We identify different patterns of V2 Ab induction by SHIV infection and three separate vaccine regimens that aid in fine-tuning an optimized immunization protocol for inducing V2p and V2i Abs. We observe no, or weak and sporadic V2p and V2i Abs in non-vaccinated SHIV-infected NHPs, but strong V2p and/or V2i Ab responses after immunization with a V2-targeting vaccine protocol. The V2-focused vaccination is superior to both natural infection and to immunization with whole Env constructs for inducing functional V2p- and V2i-specific responses. Strikingly, levels of V2-directed Abs correlate inversely with Abs specific for peptides of V3 and C5. These data demonstrate that a V1V2-targeting vaccine has advantages over the imprecise targeting of SIV/SHIV infections and of whole Env-based immunization regimens for inducing a more focused functional V2p- and V2i-specific Ab response.