bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene
Authors: Novakova Zora , Milosevic Mirko , Kutil Zsofia , Ondrakova Marketa , Havlinova Barbora , Kasparek Petr , Sandoval-Acuña Cristian , Korandova Zuzana , Truksa Jaroslav , Vrbacky Marek , Rohlena Jakub , Barinka Cyril 
Published: March 2022

Abstract
In humans, disruptions in the heme biosynthetic pathway are associated with various types of porphyrias, including variegate porphyria that results from the decreased activity of protoporphyrinogen oxidase IX (PPO; E.C.1.3.3.4), the enzyme catalyzing the penultimate step of the heme biosynthesis. Here we report the generation and characterization of human cell lines, in which PPO was inactivated using the CRISPR/Cas9 system. The PPO knock-out (PPO-KO) cell lines are viable with the normal proliferation rate and show massive accumulation of protoporphyrinogen IX, the PPO substrate. Observed low heme levels trigger a decrease in the amount of functional heme containing respiratory complexes III and IV and overall reduced oxygen consumption rates. Untargeted proteomics further revealed dysregulation of 22 cellular proteins, including strong upregulation of 5-aminolevulinic acid synthase, the major regulatory protein of the heme biosynthesis, as well as additional ten targets with unknown association to heme metabolism. Importantly, knock-in of PPO into PPO-KO cells rescued their wild-type phenotype, confirming the specificity of our model. Overall, our model system reveals physiological consequences of the PPO ablation at the cellular level and can serve as a tool to study various aspects of dysregulated heme metabolism associated with variegate porphyria.
Ficoll density gradient sedimentation isolation of pelage hair follicle mesenchymal stem cells from adult mouse back skin: A novel methods for hair follicle mesenchymal stem cells isolation.
Authors: Gan Yuyang , Wang Hailin , Du Lijuan , Fan Zhexiang , Sun Pingping , Li Kaitao , Qu Qian , Wang Jin , Chen Ruosi , Hu Zhiqi , Miao Yong 
Published: March 2022

Abstract
Background: Hair follicle mesenchymal stem cells(HF-MSC) has great potential for cell therapy. Traditional method to isolate whisker HF-MSC is time consuming and few in cell numbers. How to quickly and conveniently obtain a large number of HF-MSC for experimental research is a problem worth exploring.Methods: Two steps Ficoll Density Gradient Sedimentation(FDGS) were performed to isolated pelage HF-MSC from adult mice. The characteristic of the isolated cells were identified and compared with whisker HF-MSC by immunofluorescence staining, flow cytometry, three-lineage differentiation and hair follicle reconstruction. Pelage HF-MSC were injected into the dorsal skin of mice to explore its role in promoting hair growth. The cells and exosomes distribution were located by immunofluorescence staining.Results: Isolated pelage HF-MSC expressed similar markers (ALP, Versican, NCAM, Nestin), showed similar growth pattern, possessed similar mesenchymal stem cells function and hair follicle induction ability as whisker HF-MSC. A large number of cells can be obtained with fewer mice compared to tranditional method. Pelage HF-MSC injection can promote hair growth. Pelage HF-MSC were distributed surround the host hair follicle, secreting exosomes that integrated into host hair follicle and located in dermal papillae and hair matrix region. Conclusion: A large number of Pelage HF-MSC can be isolated by FDGS, which can promote hair growth by secreting exosomes which targeted the dermal papilla and hair matrix region of host hair follicle.
Cluster B personality disorders among psychiatric outpatients in Southwest Ethiopia: Institution-based cross-sectional study
Authors: Jemal Muzeyen , Tessema Worknesh , Agenagnew Liyew 
Published: March 2022

Abstract
Background: Diagnosis of co-occurring personality disorders, particularly the most comorbid cluster B personality disorders in psychiatric patients is clinically important because of their association with the duration, recurrence, and outcome of the comorbid disorders. The aim of the study was to assess the prevalence of cluster B personality disorders and associated factors among psychiatric outpatients in Jimma Medical Center.Methods: An institution-based cross-sectional study was conducted among 404 patients with mental illnesses at Jimma Medical Center, psychiatry clinic, from July 15 to September 14, 2021. A systematic random sampling method was used to recruit the participants. Personality disorder questionnaire four (PDQ-4) was used to assess the prevalence of cluster B personality disorders through a face-to-face interview. Data were entered into Epi Data Version 4.6 and exported to SPSS Version 26 for analysis. Logistic regression analysis was done and variables with a p-value less than 0.05 with a 95% confidence interval in the final fitting model were declared as independent predictors of cluster B personality disorders. Result: Amongst 401 respondents with response rate of 99.3%, slightly less than one-fourth (23.19%, N=93) were found to have cluster B personality disorders. Unable to read and write(AOR = 3.28, 95%CI = 1.43 - 7.51), unemployment(AOR = 2.32, 95%CI = 1.19 - 4.49), diagnosis of depressive (AOR = 3.72, 95%CI = 1.52-9.10) and bipolar-I disorders (AOR = 2.94, 95%CI = 1.37 - 6.29), longer duration of illness (AOR = 2.44, 95%CI = 1.33 - 4.47), multiple relapses (AOR = 2.21, 95%CI = 1.18–4.15)), history of family mental illnesses (AOR = 2.05, 95%CI = 1.17 - 3.62), recent cannabis use (AOR = 4.38, 95%CI = 1.61 - 11.95), recent use of alcohol (AOR = 2.86, 95%CI = 1.34 - 6.10), starting to use substance at earlier age (AOR = 4.42, 95%CI = 1.51 -12.96), and suicidal attempt (AOR = 2.24, 95%CI= 1.01 - 4.96) , were the factors significantly associated with cluster B personality disorders.Conclusion: The prevalence of cluster B personality disorders was high among mentally ill outpatients and found to be important for mental health professionals working in the outpatient departments to screen for the cluster B personality disorders as part of their routine activities, particularly those who have mood disorders, longer duration of illness, multiple relapses, history of family mental illnesses, suicidal attempt and are a current user of alcohol and cannabis.
Morphometric Considerations on the Inferior Thyroid Artery With Its Origin in the Subclavicular Artery Determined by Ct Examination
Authors: Bunea Maria Cristina , Rusali Lavinia Maria , Bratu Iulian Cătălin , Tudorache Sorin , Bordei Petru 
Published: March 2022

Abstract
In the specialized literature, there is a low degree of information regarding the morphometric aspects of the inferior thyroid artery. The study was performed on CTAs, and the following was observed: the distance between the origin of the inferior thyroid artery in relation to the origin of the corresponding subclavian artery, as well as the artery diameter comparing right to left, according to gender and in comparison with the diameter of the subclavicular artery or thyrocervical trunk from which it originated.The study was performed on a total of 108 inferior thyroid arteries (64 on the right side and 44 on the left side, 48 in males subjects and 60 arteries in female subjects), analyzed on computed tomography angiography. Of the 108 arteries, the origin of the inferior thyroid directly from the subclavian artery was found in 34 cases, 20 cases being on the right side and 14 cases on the left side. In the other 74 cases, the origin of the inferior thyroid artery was in a thyrocervical trunk, 44 cases being on the right side, and 30 cases on the left side. The distance and mean diameters are variable between genders and the left/right side.The inferior thyroid artery is one of the arteries most susceptible to variations in terms of origin and size. With differences between the two sides (right and left), as well as differences related to gender.
Nitrogen Content and C/N Ratio in Straw are the Key to Affect Biological Nitrogen Fixation in a Paddy Field
Authors: Zhang Yanhui , Hu Tianlong , Wang Hui , Jin Haiyang , Liu Qi , Chen Zhe , Xie Zubin 
Published: March 2022

Abstract
Background and aims
Straw amendment can increase nitrogen fixation in paddy field, however, the efficiency of carbon sources with different biochemical properties to enhance N2 fixation and nitrogen fixation activity is still unclear.
Methods
A 15N2-labelling system was used in the field environment to determine biological nitrogen fixation (BNF) under the addition of three different carbon substrates. The nifH gene (DNA) and nifH RNA gene (cDNA) of soil were amplified by real-time fluorescent quantitative PCR to study the number of nitrogen fixing microorganisms and nifH gene expression. The diversity and community composition of nitrogen fixing microorganisms were studied by high-throughput sequencing of nifH DNA gene. The study is expected to reveal how different carbon sources impact biological nitrogen fixation and its mechanism in the paddy field system.
Results
Results showed that the absolute abundance (nifH DNA gene copy numbers) of heterotrophic diazotrophs in the treatment of mature stage wheat straw (MWS) was 4.88 times as high as that in the CK treatment, but jointing stage wheat straw (JWS) and poplar branch (PB) did not induce any significant changes. Straw amendment had no impact on cyanobacteria abundance. The proportion of N2 fixation increased by MWS was 2.07 times, but diazotrophic nitrogen fixation activity decreased. The enhanced N2 fixation by MWS compared with JWS and PB was due to its higher C/N ratio.
Conclusions
Low nitrogen content and high C/N ratio are vital to increase N2 fixation, but the efficiency of straw amendment on N2 fixation might not be as high as expected.
Uveitis Associated with Juvenile Idiopathic Arthritis: A Continued Cohort Study 40 Years After Uveitis Onset
Authors: Skarin Angelika , Berthold Elisabet , Rauer Ola , Bengtsson-Stigmar Elisabeth 
Published: March 2022

Abstract
Background: A third follow-up study, mean 40.7 years after uveitis onset, of a cohort originally consisting of 55 Swedish patients with uveitis associated with Juvenile Idiopathic Arthritis (JIA).Method: A retrospective study of the patients’ ophthalmic medical records. The results were compared to those of the same cohort previously studied at mean 7.2 and 24.0 years after uveitis onset. In the present follow-up study, 30 of the original 55 patients consented to participate. Of these, 26 had ophthalmic medical records that were reviewed. Results: In the 30 participants, active uveitis was seen in 43.4%, cataracts in 66.6% and glaucoma in 40.0%. When comparing data from previous follow-ups of the same cohort, a total of 61.8% were reported to have had cataracts at any of the three follow-ups, 29.0% had glaucoma or ocular hypertension and 12.7% had severe visual impairment in both eyes. At mean 40.7 years after uveitis onset 20% of patients in the original uveitis cohort were deceased. In 4 of the 11 deceased individuals, rheumatic disease was stated as the main cause of death, and in 3 it was considered a contributory factor in the patients deaths. Conclusions: Uveitis associated with JIA can be active into midlife and possibly longer. Ocular complications and visual loss increased up to 40 years after uveitis diagnosis. The mortality rate of this cohort was higher than that of a corresponding Swedish population. Lifelong ophthalmic check-ups are probably necessary for patients diagnosed with this type of uveitis.
Factors associated with contraceptive failure in Uganda: Analysis of the 2016 Uganda Demographic and Health Survey
Authors: Kisuza Ruth Ketty , Kicaber Saviour , Abila Derrick Bary , Bongomin Felix , Garimoi Christopher Orach 
Published: March 2022

Abstract
BackgroundSustained motivation is essential for effective use of contraceptive methods by women in low- and middle-income countries as many women are likely to abandon use of contraceptives especially when they continually experience episodes of failure. We aimed to determine contraceptive failure rates and associated factors among Ugandan women using data from the 2016 Uganda Demographic Health Survey (UDHS).MethodsWe analyzed data collected by the UDHS conducted in Uganda 2016. All eligible women aged 15 to 49 years at the time of the survey were enrolled. Discontinuation of contraceptive use due to failure within a 5-year period preceding the survey was the dependent variable.ResultsA total of 18,505 women were included in this study, 70.8% (n=5153) lived in rural areas while 56.9% (n=5153) owned a mobile phone. The mean age of the women was 29.6years (SD 7.6). The overall prevalence of contraceptive failure was 5.6%, and was higher (7.8%) among women aged 25-29 years or had completed secondary education (7.1%). The odds of contraceptive failure was 38% lower in women who had an informed choice on contraceptives compared to those who didn’t [Adjusted Odd ratio, 0.62; 95% confidence interval, 0.50 – 0.77; p< 0.001].ConclusionThe burden of contraceptive failure among women of reproductive age in Uganda is substantial and significantly varied by socio-demographic characteristics.
Quantification of cell contractile behavior based on non-destructive macroscopic measurement of tension forces on bioprinted hydrogel
Authors: Pragnere Sarah , Kholti Naima El , Gudimard Leslie , Essayan Lucie , Marquette Christophe , Petiot Emma , Pailler-Mattei Cyril 
Published: March 2022

Abstract
Contraction assay based on surface measurement have been widely used to evaluate cell contractility in 3D models. This method is straightforward and requires no specific equipment, but it does not provide quantitative data about contraction forces generated by cells. We expanded this method with a new biomechanical model, based on the work-energy theorem, to provide non-destructive longitudinal monitoring of contraction forces generated by cells in 3D. We applied this method on hydrogels seeded with either fibroblasts or osteoblasts. Hydrogel mechanical characteristics were modulated to enhance (condition HCAHigh: hydrogel contraction assay high contraction) or limit (condition HCALow: hydrogel contraction assay low contraction) cell contractile behaviors. Macroscopic measures were further correlated with cell contractile behavior and descriptive analysis of their physiology in response to different mechanical environments. Fibroblasts and osteoblasts contracted their matrix up to 47% and 77% respectively. Contraction pressure peaked at day 5 with 1.1 10-14 Pa for fibroblasts and 3.5 10-14 Pa for osteoblasts, which correlated with cell attachment and spreading. Negligible contraction was seen in HCALow. Both fibroblasts and osteoblasts expressed α-SMA contractile fibers in HCAHigh and HCALow. Failure to contract HCALow was attributed to increased cross-linking and resistance to proteolytic degradation of the hydrogel.
The Diversity and Function of The in Situ Fungal Communities in Response to Polycyclic Aromatic Hydrocarbons in The Urban Wetland
Authors: Wu Huanling , Pan Shaobing , Sun Binghua , Li Jinhua 
Published: March 2022

Abstract
PAHs (Polycyclic Aromatic Hydrocarbons) increases the potential harm to ecosystem and human health. The fungi is considered as a powerful choice for degradation of PAHs. The researches on the effect of PAHs on fungal population in sediment/soil mostly stayed in the laboratory simulation that based on extreme pollution. This study investigated the fungal population of the urban wetland by high-throughput sequencing in situ micro-pollution state. Our statistical analysis revealed significant difference in the whole fungal population at the phylum among the three land use types. Among them, Ascomycota was the dominant fungi at the phyla in three land use types. Fungal genus of degrading PAHs were significantly correlated with Dibenz[a, h]anthracene (P = 0.018) in ditch wetland, Total Organic Carbon (P = 0.02) and Fluoranthene (P = 0.04) in riverine wetland, and Electrical Conductivity (P = 0.018) in agricultural land. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) suggested that 20 enzymes were present related to PAHs metabolism in three land use types. Specifically, monoxygenase, dehydrogenase, and laccase were most abundant among inferred enzymes, indicating that the urban wetland had potential for the degradation of PAHs. This study contributed to in-depth understanding of the structure and function of fungal population and provided a theoretical basis for PAHs microbial remediation in the in-situ environment.
Endophytic Fungi Isolation and Identification from Stem of Tinospora Cordifolia
Authors: Dinodia Neelam , Sharma Rajesh K. , Gond Vijay K. , Dinodia Neelam 
Published: March 2022

Abstract
Endophytes refer to the microorganisms (mostly fungi and bacteria) colonising the intercellular and intracellular regions of healthy plant tissues at a particular time, whose presence is unobtrusive and asymptomatic. Fungal endophytes are believed to be treasure of structurally and biologically active compounds. Sometimes, the medicinal properties of plants result from the endophytes present in the host plant and the type of secondary metabolites produced by those endophytes. The present study was conducted with a culture-dependent based approach in order to isolate, identify endophytic fungi from stem of Tinospora cordifolia i.e. common medicinal plant Giloy. In this study a total of nine endophytic fungal isolates were obtained. All isolates were primarily identified by morphological characteristics and then were subjected to single spore isolation process where distinguished culture of every individual colony was prepared and phyto-genetically examined by molecular experiments. The endophytic fungi were velvety reddish white or grey, cottony and woolly grey or pink growth with spores touching the lid converted the agar into red, grey, pink colour. The growth characteristics of endophytic fungal isolates in Potato dextrose broth showed mycelia formation at the surface with colouration of the broth as seen on the agar. Molecular characterization was done by Polymerase chain reaction and gene sequencing of the internal transcribed spacer regions of the rDNA gene which revealed that the endophytic fungi isolates from Tinospora cordifolia stem belonged to Phoma sojicola and Lasiodiploda theobromae. Acknowledgements: We are thankful to the department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Jabalpur, Madhya Pradesh, India 482001 for providing necessary facilities to carry out the research work. ORCHIT: 0000-0001-5517-4161
The changing characteristics of post-COVID-19 syndrome: Cross-sectional findings from 458 consultations using the Stanford Hall remote rehabilitation assessment tool
Authors: O'Sullivan Oliver 
Published: March 2022

Abstract
Background In the UK, there have been multiple waves of coronavirus disease 2019 (COVID-19), with a five-tier alert system created to describe the transmission rate and appropriate restrictions. While acute mortality decreased, there continues to be significant morbidity, with individuals suffering persistent, life-restricting symptoms for months-to-years afterwards. A remote rehabilitation tool was created at the Defence Medical Rehabilitation Centre (DMRC) Stanford Hall to assess post-COVID-19 symptoms and their impact on the UK Military. This study aims to understand changes in post-COVID-19 syndrome between wave one and wave two, identify interactions between alert level and symptoms, and investigate any predictive nature of acute symptoms for post-acute symptomology in a young, physically-active population. Methods Cross-sectional study of 458 consecutive remote rehabilitation assessments performed at DMRC Stanford Hall between 2/4/2020 and 29/7/2021. Consultations were coded, anonymised, and statistical analysis was performed to determine associations between acute and post-acute symptoms, and between symptoms, alert level, and wave. Results 435 assessments were eligible; 174 in wave one and 261 in wave two. Post-COVID-19 syndrome prevalence reduced from 43% to 2% between the waves. Acutely, widespread pain was more prevalent in wave two (P<0.001). Post-acutely, there was increased anxiety (p=0.10) in wave one and increased sleep disturbance (p<0.001), memory/concentration issues (p<0.001), and shortness of breath/cough (p=0.017) in wave two. Increasing alert level was associated with increased post-acute symptom prevalence (p=0.046), with sleep disturbance increasing at higher alert level (p=0.016). Acute symptoms, including fatigue, sleep disturbance and myalgia, were associated with multiple post-acute symptoms. Conclusions This study reports the overall prevalence and symptom burden in the UK Military in the first two waves of COVID-19. By reporting differences in COVID-19 in different waves and alert level, this study highlights the importance of careful assessment and contextual understanding of acute and post-acute illness for individual management plans.
Evidence Based on Global One Health Index: An Assessment Tool for One Health Performance
Authors: Xiao-Xi ZHANG , Jing-Shu LIU , Le-Fei HAN , Shang XIA , Shi-Zhu LI , Y. LI Odel , Kokouvi KASSEGNE , Min LI , Kun YIN , Qin-Qin HU , Le-Shan XIU , Yong-Zhang ZHU , Liang-Yu HUANG , Xiang-Cheng WANG , Yi ZHANG , Han-Qing ZHAO , Jing-Xian YIN , Tian-Ge JIANG , Qin LI , Si-Wei FEI , Si-Yu GU , Fu-Min CHEN , Nan ZHOU , Zi-Le CHENG , Yi XIE , Hui-Min LI , Jin CHEN , Zhao-Yu GUO , Jia-Xin FENG , Lin AI , Jing-Bo XUE , Qian YE , Liz GRANT , Jun-Xia SONG , Geoff SIMM , Jürg UTZINGER , Xiao-Kui GUO , Xiao-Nong ZHOU 
Published: March 2022

Abstract
Background
A One Health approach has been increasingly mainstreamed by the international community, as it provides the holistic thinking in recognizing the close links and inter-dependence of the health of humans, animals and the environment. The dearth of real-world evidence has hampered application of a One Health approach in shaping policies and practice. A consolidated evaluation tool is urgently needed for valid assessment of One Health approaches.
Methods
We develop the Global One Health Index (GoHi) by five steps, including framework formulation, indicator selection, database building, weight determination and GoHi scores calculation. A Cell-like Framework for GoHi is proposed, which comprises an External Driver Index (EDI), an Intrinsic Driver Index (IDI) and a Core Driver Index (CDI). We construct the indicator scheme for GoHi based on this framework after multiple rounds of panel discussions with our Expert Advisory Committee. A Fuzzy Analytical Hierarchy Process (FAHP) is adopted to determine the weights for indicators.
Results
The weighted indicator scheme of GoHi comprises 3 first-level indicators, 13 second-level indicators, and 57 third-level indicators. The results from a pilot analysis are consistent with the results from a literature review, which suggests the feasibility of GoHi as a tool for the assessment of global One Health performance. GoHi is the world’s first evaluation tool that constructs the conceptual framework from a holistic perspective of One Health, and captures the data from more than 200 countries/ territories for empirical analysis. The application of GoHi will promote a common understanding of a strong One Health approach and provide a means for identifying gaps and promoting effective measures to strengthen One Health capacity building.
Conclusions
We propose GoHi as a potential systematic evaluation tool for global One Health approaches. With further adaptations in various scenarios, GoHi along with its technical protocols and databases, will be updated regularly to address current technical limitations, and capture new knowledge. Meanwhile, stronger political endorsement and facilitation are essential for the development of One Health policies, practice and research.