bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gut microbiota-derived ursodeoxycholic acid from neonatal dairy calves improves intestinal homeostasis and colitis to attenuate extended-spectrum β-lactamase-producing enteroaggregative Escherichia coli infection
Authors: He Zhiyuan , Ma Yulin , Yang Sirui , Zhang Shuyuan , Liu Shuai , Xiao Jianxin , Wang Yajing , Wang Wei , Yang Hongjian , Li Shengli , Cao Zhijun 
Published: May 2022

Abstract
Background
Antimicrobials are often used to prevent and treat diarrhea induced by enteroaggregative Escherichia coli (EAEC) in young ruminants. However, drug overuse or misuse accelerates the spread of multidrug-resistant extended-spectrum β-lactamase (ESBL)-producing E. coli. Thus, supplementary foods as alternatives to antibiotics are needed to prevent colibacillus diarrhea in neonatal dairy calves. Ursodeoxycholic acid (UDCA), a therapeutic bile acid, helps alleviate colitis. However, how UDCA helps alleviate ESBL-EAEC-induced clinical symptoms and colitis remains unclear.

Results
We investigated the microbial profiles and metabolites of healthy and diarrheic neonatal calves to determine microbial and metabolite biomarkers in early-life development. Both the gut microbiota communities and their associated metabolites differed between healthy and diarrheic calves. Commensal Butyricicoccus, Faecalibacterium, Ruminococcus, Collinsella, and Coriobacterium were key microbial markers that distinguished healthy and diarrheic gut microbiomes. Random forest machine-learning algorithm and Spearman correlation results indicated that enriched UDCA, short-chain fatty acids (SCFAs), and other prebiotics were strongly positively correlated with these five bacterial genera. We explored the effect of ursodiol on bacterial growth, cell adherence, and lipopolysaccharide-treated Caco-2 cells. Adding ursodiol induced direct antibacterial effects, suppressed proinflammatory effects, and reduced cell integrity damage. Oral ursodiol delivery to neonatal mice exhibited significant antibacterial effects and helped maintain colonic barrier integrity in mouse models of peritonitis sepsis and oral infection. UDCA supplementation attenuated colitis and recovered colonic SCFA production. To validate this, we performed fecal microbiota transplantations to inoculate ESBL-EAEC-infected neonatal mice. Microbiotas from UDCA-treated neonatal mice ameliorated colitis and hindgut commensal bacterial damage compared with that of the microbiotas from the control and placebo mice, as evidenced by colonization of abundant bacteria, including Oscillospiraceae, Ruminococcaceae, Lachnospiraceae, and Clostridia_UCG-014, and upregulated SCFA production.

Conclusions
This study provided the first evidence that UDCA could confer diarrhea resistance in ESBL-EAEC-infected newborn dairy calves. UDCA blocked bacterial growth and invasion both in vitro and in vivo, alleviated commensal bacterial dysbiosis during ESBL-EAEC infection in neonatal mouse models of sepsis and colitis via the TGR5-NF-κB axis, and upregulated SCFA production in the hindgut digesta. Our findings provide insight into the UDCA-mediated remission of ESBL-EAEC infections and the potential role of UDCA as an antibiotic alternative.
How to use biogas?: A systematic review of biogas utilization pathways and business models
Authors: Mertins Anica , Wawer Tim 
Published: May 2022

AbstractThere are many options for the utilization of biogas in different energy sectors (power, heat, mobility). The technical possibilities of using biogas are more diverse than the actual business models applied in the biogas industry. This paper shows the possible utilization pathways of biogas, divided into coupled power and heat generation, direct utilization and upgrading to a gas of a higher value. Subsequently, an overview of the business models discussed is given by a systematic literature review. The latter shows that the investigation of biogas business models is focused mainly on the last decade and has increased slightly over time. The regions of investigation can be found worldwide, with a clear focus on Europe. Direct use is studied mainly in the Asian and African regions. In the European context, a shift from investigating combined heat and power use to upgrading the biogas produced is evident.
Fruit recognition method for a harvesting robot with RGB-D cameras
Authors: Yoshida Takeshi , Kawahara Takuya , Fukao Takanori 
Published: May 2022

AbstractIn this study, we present a recognition method for a fruit-harvesting robot to automate the harvesting of pears and apples on joint V-shaped trellis. It is necessary to recognize the three-dimensional position of the harvesting target for harvesting by the fruit-harvesting robot to insert its end-effector. However, the RGB-D (red, green, blue and depth) camera on the harvesting robot has a problem in that the point cloud obtained in outdoor environments can be inaccurate. Therefore, in this study, we propose an effective method for the harvesting robot to recognize fruits using not only three-dimensional information obtained from the RGB-D camera but also two-dimensional images and information from the camera. Furthermore, we report a method for determining the ripeness of pears using the information on fruit detection. Through experiments, we confirmed that the proposed method satisfies the accuracy required for a harvesting robot to continuously harvest fruits.
ID-Seg: an infant deep learning-based segmentation framework to improve limbic structure estimates
Authors: Wang Yun , Haghpanah Fateme Sadat , Zhang Xuzhe , Santamaria Katie , da Costa Aguiar Alves Gabriela Koch , Bruno Elizabeth , Aw Natalie , Maddocks Alexis , Duarte Cristiane S. , Monk Catherine , Laine Andrew , Posner Jonathan ,   program collaborators for Environmental influences on Child Health Outcomes
Published: May 2022

AbstractInfant brain magnetic resonance imaging (MRI) is a promising approach for studying early neurodevelopment. However, segmenting small regions such as limbic structures is challenging due to their low inter-regional contrast and high curvature. MRI studies of the adult brain have successfully applied deep learning techniques to segment limbic structures, and similar deep learning models are being leveraged for infant studies. However, these deep learning-based infant MRI segmentation models have generally been derived from small datasets, and may suffer from generalization problems. Moreover, the accuracy of segmentations derived from these deep learning models relative to more standard Expectation–Maximization approaches has not been characterized. To address these challenges, we leveraged a large, public infant MRI dataset (n = 473) and the transfer-learning technique to first pre-train a deep convolutional neural network model on two limbic structures: amygdala and hippocampus. Then we used a leave-one-out cross-validation strategy to fine-tune the pre-trained model and evaluated it separately on two independent datasets with manual labels. We term this new approach the Infant Deep learning SEGmentation Framework (ID-Seg). ID-Seg performed well on both datasets with a mean dice similarity score (DSC) of 0.87, a mean intra-class correlation (ICC) of 0.93, and a mean average surface distance (ASD) of 0.31 mm. Compared to the Developmental Human Connectome pipeline (dHCP) pipeline, ID-Seg significantly improved segmentation accuracy. In a third infant MRI dataset (n = 50), we used ID-Seg and dHCP separately to estimate amygdala and hippocampus volumes and shapes. The estimates derived from ID-seg, relative to those from the dHCP, showed stronger associations with behavioral problems assessed in these infants at age 2. In sum, ID-Seg consistently performed well on two different datasets with an 0.87 DSC, however, multi-site testing and extension for brain regions beyond the amygdala and hippocampus are still needed.
Dietary inflammatory potential and the incidence of depression and anxiety: a meta-analysis
Authors: Li Xiaoling , Chen Meicui , Yao Zhicui , Zhang Tianfeng , Li Zengning 
Published: May 2022

AbstractThe potential modifiable role of diet in common psychological disorders, including depression and anxiety, has attracted growing interest. Diet may influence the occurrence of mental disorders through its inflammatory characteristics. The purpose of this meta-analysis was to explore whether dietary inflammatory potential is associated with the risk of depression and anxiety. A systematic literature search was conducted in PubMed, Web of Science, and Embase databases up to February 2021. Articles related to dietary inflammatory potential and risk of depression or anxiety were included. After the elimination of repetitive and irrelevant literature, we conducted quality assessment, publication bias, and sensitivity analysis. In total, 17 studies with a total of 157,409 participants were included in the final analysis. Compared with the lowest inflammatory diet group, the highest group was significantly associated with the incidence of depression and anxiety, with the following pooled odds ratios (ORs) and 95% confidence intervals (95% CIs): 1.45 (1.30 ~ 1.62) for depression and 1.66 (1.41 ~ 1.96) for anxiety. A subgroup analysis by gender showed that this association was more prominent in women. For depression, the increased risk was 49% in women (OR 1.49, 95% CI 1.28 ~ 1.74) and 27% in men (OR 1.27, 95% CI 1.06 ~ 1.52). As for anxiety, the increased risk was 80% in women (OR 1.80, 95% CI 1.30 ~ 2.49) and 47% in men (OR 1.53, 95% CI 0.81 ~ 2.89). As a result, long-term anti-inflammatory eating patterns may prevent depression and anxiety, whereas pro-inflammatory eating patterns may promote these conditions. People should add more fish, fish oil, fresh fruit, walnuts, and brown rice to their diet.
Content validity of a newly developed observer-reported measure for pediatric asthma in children aged 2–5 years
Authors: Zhang Yi , Clegg Jennifer L. , Keith Shannon , McFadden Shehan , Symonds Tara , Kumar Rajesh , Khan Asif H. , Kamat Siddhesh , Chao Jingdong 
Published: May 2022

Abstract
Background
An observer-reported outcome (ObsRO) measure assessing both symptom control and health-related quality of life (HRQoL) in children with asthma younger than 6 years is lacking. The objective of this study was to evaluate the content validity of the Pediatric Asthma Questionnaire (PAQ), a newly developed 6-item ObsRO measure for caregivers of children aged 2–5 years diagnosed with asthma.

Results
In-depth, qualitative interviews were conducted with 15 parents or caregivers. The first part of the interview was an open-ended discussion whereby participants were asked to describe their observations of their child’s asthma symptoms and HRQoL impacts followed by a cognitive debriefing of a draft version of the PAQ. The most frequently reported symptoms were coughing (n = 15, 100%), wheezing (n = 14, 93%), and trouble breathing (n = 10, 67%). Overall, participants found the PAQ easy to complete and relevant to their child’s experience with asthma, with most reporting the instructions, response scales, and recall period for the items to be appropriate. The majority of participants (93%) believed they could accurately report on the items included in the PAQ based on their observations of their child’s asthma symptoms and impacts, or reliably get the information from the child’s teacher, school, or caregiver when their child was not in their presence. One item was modified based on feedback about the phrase “oral steroids” to clarify modes of administration. A few other minor changes were incorporated into the PAQ following suggestions from participants, including replacing the phrase “how often” with “how many days” in one of the items to improve clarity and overall consistency with the response options.

Conclusion
Qualitative data support the content validity of the PAQ as a fit-for-purpose and well-understood 6-item observer-reported outcome measure to evaluate both symptoms and asthma-specific HRQoL impacts experienced by pediatric asthma patients aged 2–5 years for use in clinical and real-world studies.
Development and content validity testing of patient-reported outcome (PRO) items to assess chest congestion associated with the common cold for use in children and adolescents
Authors: Arbuckle Rob , Marshall Chris , Grant Laura , Burrows Kate , Albrecht Helmut H. , Shea Tim 
Published: May 2022

Abstract
Background
This article describes qualitative interviews conducted with children (aged 6–11), adolescents (aged 12–17), and adults with the common cold as well as parents/caregivers of the 6–8-year-old children. The aim was to support the refinement and content validity testing of patient-reported outcome (PRO) items assessing chest congestion that could be used as pediatric clinical trial endpoints. Feasibility and acceptability of administering the PRO items electronically on a hand-held touch-screen device were also evaluated. The sample included children aged 6–8 years (n = 14), 9–11 years (n = 13), adolescents aged 12–17 years (n = 12), and adults (n = 10), all of who had current (n = 38) or recent (n = 11) cold. Both concept elicitation (CE) and cognitive debriefing (CD) interviews were conducted with all of these participants, conducted over in two rounds. Ten parents/caregivers of participants aged 6–8 years were also interviewed (separately from their child) regarding how they thought their children would understand the items. The CE interviews explored the qualitative experience of having chest congestion and related symptoms of the common cold. Following their CE interview, participants completed draft items on an electronic patient-reported outcome (ePRO) device twice daily for 2–5 days prior to their CD interview. During the CD interview participants were asked about relevance, understanding and interpretation of the draft PRO items. Qualitative analysis of the interview data and descriptive analyses of the ePRO data were conducted following both rounds of interviews, with modifications to the items implemented following Round 1 and tested in Round 2.

Results
Eight symptoms were reported by children during concept elicitation. Findings from the child, adolescent, and adult/parent interviews supported revisions to the items and enabled the selection of the best performing items. The results provided evidence that the final items were well understood by participants and relevant to their experiences of chest congestion as part of a common cold. Findings also provide support for using the same items across age groups.

Conclusions
The results of the CE and CD interviews provide evidence supporting the content validity of new PRO items assessing the experience of chest congestion symptoms associated with common cold experienced by children, adolescents, and adults.
The role of angiotensin-converting enzyme 2 (ACE2) genetic variations in COVID-19 infection: a literature review
Authors: Fawzy Manal S. , Ashour Hend , Shafie Aya Allah Ashraf , Dahman Nesrine Ben Hadj , Fares Abdelhamid M. , Antar Sarah , Elnoby Ahmed S. , Fouad Fatma Mohamed 
Published: May 2022

Abstract
Background
The angiotensin-converting enzyme-2 (ACE2) is recognized to be the fundamental receptor of severe acute respiratory syndrome coronavirus-2 (SARS-CoV2), responsible for the worldwide Coronavirus Disease-2019 (COVID-19) epidemic. However, genetic differences between people besides racial considerations and their relation to disease susceptibility are still not fully elucidated.

Main body
To uncover the role of ACE2 in COVID-19 infection, we reviewed the published studies that explore the association of COVID-19 with the functional characteristics of ACE2 and its genetic variations. Notably, emerging studies tried to determine whether the ACE2 variants and/or expression could be associated with SARS-CoV/SARS-CoV2 have conflicting results. Some researchers investigated the potential of “population-specific” ACE2 genetic variations to impact the SARS-CoV2 vulnerability and suggested no ethnicity enrichment for ACE2 polymorphisms that could influence SARS-CoV2 S-protein binding. At the same time, some studies use data mining to predict several ACE2 variants that could enhance or decline susceptibility to SARS-CoV. On the other hand, fewer studies revealed an association of ACE2 expression with COVID-19 outcome reporting higher expression levels of ACE2 in East Asians.

Conclusions
ACE2 gene variants and expression may modify the deleterious consequences of SARS-CoV2 to the host cells. It is worth noting that apart from the differences in gene expression and the genetic variations of ACE2, many other environmental and/or genetic factors could modify the disease outcome, including the genes for the innate and the adaptive immune response.
Recto-vestibular fistula with colonic duplication: report of three cases and review of literature
Authors: Piplani Rajat , Acharya Samir K. , Bagga Deepak 
Published: May 2022

Abstract
Background
Anorectal malformations associated with colonic duplications are a rare condition. Double terminations of tubular colonic duplication in the perineum are even rarer. Colonic duplication may have different presentations according to its location and size and incidence of less than 15% of all gastrointestinal duplications. We hereby report three such cases of recto-vestibular fistula along with underlying tubular colorectal duplication over a period of 10 years (2010–2020) managed successfully by different surgical approaches. These cases also illustrate the diagnostic challenge and variable presentation of colonic duplications in female patients with recto-vestibular fistula.

Case presentation
Case 1 is a patient with flank heteropagus with absent anal opening and recto-vestibular fistula, while cases 2 and 3 presented as suspected H-type recto-vestibular fistula with normal anal opening. All the cases were however managed with a specific surgical approach on case basis. Duplicated colon was excised in case 1, but in case 2 and case 3, duplicated colon was converted into single channel using proximal and distal staplers. Further definitive repair of female recto-vestibular fistula was done.

Conclusions
Colorectal duplication is a rare congenital malformation, and their association with anorectal malformations is even rarer. This association can have varied presentation, and surgical approach should be according to the congenital anatomical variations on case-to-case basis. Colonic duplications should always be ruled out in a female newborn child with history of passage of stools both from the vestibule and anus.
A living tool for the continued exploration of microplastic toxicity
Authors: Thornton Hampton Leah M. , Lowman Heili , Coffin Scott , Darin Emily , De Frond Hannah , Hermabessiere Ludovic , Miller Ezra , de Ruijter Vera N. , Faltynkova Andrea , Kotar Syd , Monclús Laura , Siddiqui Samreen , Völker Johannes , Brander Susanne , Koelmans Albert A. , Rochman Chelsea M. , Wagner Martin , Mehinto Alvine C. 
Published: May 2022

AbstractThroughout the past decade, many studies have reported adverse effects in biota following microplastic exposure. Yet, the field is still emerging as the current understanding of microplastic toxicity is limited. At the same time, recent legislative mandates have required environmental regulators to devise strategies to mitigate microplastic pollution and develop health-based thresholds for the protection of human and ecosystem health. The current publication rate also presents a unique challenge as scientists, environmental managers, and other communities may find it difficult to keep up with microplastic research as it rapidly evolves. At present, there is no tool that compiles and synthesizes the data from these studies to allow for visualization, interpretation, or analysis. Here, we present the Toxicity of Microplastics Explorer (ToMEx), an open access database and open source accompanying R Shiny web application that enables users to upload, search, visualize, and analyze microplastic toxicity data. Though ToMEx was originally created to facilitate the development of health-based thresholds to support California legislations, maintaining the database by the greater scientific community will be invaluable to furthering research and informing policies globally. The database and web applications may be accessed athttps://microplastics.sccwrp.org/.Graphical Abstract
WEB GIS: The Promotion Model of Ngerangan Tourist Village in Klaten Regency through The Sustainable Rural Tourism Context During The Covid-19 Pandemic
Authors: Ristianti Novia Sari , Bashit Nurhadi , Ulfiana Desyta , Martono Kurniawan Teguh 
Published: May 2022

The application of large-scale social restrictions (LSSR) during the Covid 19 pandemic in Indonesia significantly impacted the tourism sector. A Sustainable Rural Tourism model is expected to develop tourist village development during the Covid 19 pandemic through the WEB GIS (Website Geographical Information Systems) approach. It creates smart tourism through tourist information systems integrated with tourist websites. Ngerangan Tourist Village in Klaten Regency is a village where most of the population's economy is dependent on MSME (Micro, Small, and Medium enterprises) and farming activities. The research aims to formulate a tourism promotion model in Ngerangan tourism village through WEB GIS. The method used is quantitative. First, identifying the data needs such as spatial data (tourist attractions, tourist safety routes types, and infrastructure for implementing health protocols) and non-spatial data (information on MSME and virtual tours). Second, designing the tourism promotion system through WEB GIS and third, testing to see the output of the WEB GIS produced. The research output is Web GIS using story maps application contains two spatial information about the Ngerangan tourist village. First, spatial information about tourist attractions includes locations, photos, and descriptions of tourist attractions. Second, spatial information regarding health protocols facilities during the Covid-19 pandemic includes locations, photos, and descriptions of health protocol facilities
ELF- or NES-oriented pedagogy: enhancing learners’ intercultural communicative competence using a dual teaching model
Authors: Taglialatela Antonio 
Published: May 2022

Abstract
This study underpins the integration of an English as a lingua franca (ELF)-oriented approach into traditional English language teaching (ELT) to develop learners’ full intercultural awareness and intercultural communicative competence (ICC). The aim is to inform prospective and senior teachers as well as practitioners about the need for adequate preparation for learners to face any speech situations involving native and non-native English speakers. Using informal classroom observations from different Italian education cycles, this study adopts a multidimensional approach to traditional ELT, combining some main ICC and ELF pedagogy tenets. Our discussion indicates that a dual teaching model, including the native English speaker (NES) model for the structural section of language teaching and learning and the ELF intercultural competent communicator model for the intercultural section, can be attained by shifting conveniently from model to model. Therefore, this study offers a fresh perspective on an extensively addressed topic by clarifying and explicitly combining the underlying connections between the main ICC and ELF tenets. This study intends to encourage teachers and practitioners to change their attitudes, perceptions, and concerns towards integrating ELF-oriented approaches into traditional ELT to tackle today’s intercultural communicative challenges outside the classroom.