bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improvement of thermal plasma sprayed coating adhesion strength by laser microtexturing of aluminum
Authors: Chakraborty Anustup , Chalfant Benjamin , Sions John , Zimmerman Ben , Gupta Mool 
Published: August 2022

Abstract

Surface microtexturing improves coating adhesion strength due to increased surface area and mechanical interlocking. Grit blasting and laser processing are two common methods used for surface microtexturing. Laser microtexturing offers distinct advantages over grit-blasting as it improves interface quality, provides a grit-particle-free surface, optimized processing time, and greater control over the surface roughness. This paper reports a full area method of laser microtexturing of Al 7075 alloy using a nanosecond pulsed laser to generate a large increase in surface area. This method involves a laser-induced thermo-mechanical process where tightly packed pillar-like surface features were formed due to the surface melting and re-solidification of materials combined with some ablation. The morphology of the microtexture was controlled by varying the laser processing parameters. Thereafter, the laser microtextured surface was coated with metallic coatings using thermal spray. Our method is superior to the currently used laser ablation-based microtexturing method. The surface morphology, composition, and adhesion strength results are presented. The tensile adhesive strength of the thermally sprayed metallic CoNiCrAlY coating was measured, and an improvement of over 17% in the coating adhesion strength was observed for a 5 µm deep microtexture compared to that of grit-blasted samples. This is the highest reported adhesion strength for thermally sprayed bond coating.
Identification and validation of SSBP1 as a key regulator involved in the development of multiple myeloma via p38MAPK signaling pathway
Authors: Xiao Pingping , Wang Jizhen , Chen Yan , Yang Apeng , Qiu Dongbiao , Chen Junmin , Zeng Zhiyong 
Published: August 2022

Abstract
Background
Multiple myeloma (MM) remains an incurable hematology malignancy. Identification of meaningful co-expressed gene clusters or representative biomarkers of MM may help to identify new pathological mechanisms and promote the development of new therapies.
Methods
Based on the expression profile and relevant clinical information of GSE6477 from the Gene Expression Omnibus (GEO) database, we performed weighted gene co-expression network analysis (WGCNA) to identify new candidate genes associated with MM development and prognosis. A series of bioinformatics analysis were used to identify the most significant hub candidate gene. In vitro and in vivo experiments further validated the function of hub gene.
Results
We conducted WGCNA with the expression profiles of GSE6477 and identified 7 meaningful co-expression modules. In the turquoise module of interest, through a series of bioinformatics analysis, SSBP1 was identified as a key regulator of MM development and related to poor prognosis. In our cohort, we also verified that MM patients with high SSBP1 expression had shorter overall survival than those with low SSBP1 expression. Furthermore, the disruption of SSBP1 expression by lentivirus mediated CRISPR/cas9 can significantly inhibit the proliferation of MM cells through inducing apoptosis and cell cycle arrest in G0/G1 phase. We also found that SSBP1 disruption significantly increased mitochondrial reactive oxygen species (mtROS) generation and the level of phosphorylated p38MAPK. Both Mito-TEMPO and SB203580 reversed the effects of SSBP1 disruption on MM cell apoptosis, the expressions of the p-p38MAPK and the apoptosis-related proteins. Mito-TEMPO also inhibited the accumulation of mtROS in MM cells caused by SSBP1 disruption, but SB203580 had no effect on mtROS. Furthermore, it was further verified that disruption of SSBP1 expression could inhibit the tumor growth via p38MAPK pathway in a human myeloma xenograft model.
Conclusion
SSBP1 promotes MM development and affects MM prognosis by regulating the p38MAPK pathway. There is evidence that SSBP1 could serve as a targetable oncogene and therapeutic biomarker for MM.
Brainstem somatostatin-expressing cells promote analgesia during negative emotional states
Authors: Winke Nanci , Aby Franck , Jercog Daniel , Girard Delphine , Grivet Zoé , Landry Marc , Castell Laia , Valjent Emmanuel , Valerio Stéphane , Fossat Pascal , Herry Cyril 
Published: August 2022

Abstract
Upon threatening situations, animals exhibit a broad range of behavioral and autonomic responses necessary for survival. Under such conditions, a crucial adaptive response is the inhibition of pain responses that would otherwise interfere with behavioral defensive responses. Whereas the structures and mechanisms involved in fear and pain behavior are well documented, little is known about the precise neuronal mechanisms mediating the emotional regulation of endogenous pain-suppression. Here we used a combination of behavioral, anatomical, optogenetic, and electrophysiological approaches to show that somatostatin-expressing cells in the ventrolateral periaqueductal gray matter (SST+ vlPAG cells) control the analgesia induced by a negative emotional state. Our data indicate that the optogenetic inhibition of SST+ vlPAG cells promotes analgesia. Conversely, the optogenetic activation of long-range SST+ vlPAG cells that project to the RVM abolishes the analgesia mediated by a negative emotional state without impacting fear behavior. Together these results identify a novel brainstem circuit mechanism composed of long-range SST+ vlPAG cells projecting to the RVM that regulate analgesia elicited by negative emotional states.
Relationship between morphometric and mechanical parameters of superficial lumbosacral soft tissue layers in healthy young adults
Authors: Grześkowiak Marcin , Kocur Piotr , Łochyński Dawid 
Published: August 2022

AbstractThe myotonometry is a noninvasive method capable to quantify linear elastic and viscoelastic properties of the myofascial tissue. However, this superficial layer contains different structures that have different distribution and organization of structural components. Myotonometric measurements of dynamic stiffness, logarithmic decrement and creep and ultrasonographic measurements of cutaneous tissue, subcutaneous tissue, thoracolumbar fascia and lumbar multifidus muscle thickness and echogenicity were obtained from 50 healthy individuals in the resting prone position and during contralateral arm lift. The most important findings were that, both in the relaxed and contracted lumbar multifidus state, the dynamic stiffness strongly negatively (r=-0.69; p<0.001 in relaxation, r=-0.83; p<0.001 in contraction) and creep strongly positively (r=0.79; p<0.001 in relaxation, r=0.85; p<0.001 in contraction) correlated with thicknesses of subcutaneous tissue. With lumbar multifidus contraction, the relative increase in dynamic stiffness was negatively correlated with relative decrease in dermis (r=-0.51; p<0.001) and subcutaneous tissue (r=-0.47; p=0.001), as well as positively correlated with relative increase in lumbar multifidus (r=0.36; p=0.010) thickness. Concluding, the amount of dynamic stiffness and creep of superficial soft tissues in the lumbar region predominantly depends on subcutaneous tissue thickness regardless of muscle state. Elasticity is barely affected by soft tissue morphometry. Mechanical parameters do not depend on the echogenicity (density) of soft tissues.
Evaluation of interactions between the hepatitis C virus NS3/4A and sulfonamidobenzamide based molecules using molecular docking, molecular dynamics simulations and binding free energy calculations
Authors: Ren Jinhong , Vaid Tasneem M. , Lee Hyun , Ojeda Isabel , Johnson Michael E. 
Published: August 2022

AbstractThe Hepatitis C Virus (HCV) NS3/4A is an attractive target for the treatment of Hepatitis C infection. Herein, we present an investigation of HCV NS3/4A inhibitors based on a sulfonamidobenzamide scaffold. Inhibitor interactions with HCV NS3/4A were explored by molecular docking, molecular dynamics simulations, and MM/PBSA binding free energy calculations. All of the inhibitors adopt similar molecular docking poses in the catalytic site of the protease that are stabilized by hydrogen bond interactions with G137 and the catalytic S139, which are known to be important for potency and binding stability. The quantitative assessments of binding free energies from MM/PBSA correlate well with the experimental results, with a high coefficient of determination, R2of 0.92. Binding free energy decomposition analyses elucidate the different contributions of Q41, F43, H57, R109, K136, G137, S138, S139, A156, M485, and Q526 in binding different inhibitors. The importance of these sidechain contributions was further confirmed by computational alanine scanning mutagenesis. In addition, the sidechains of K136 and S139 show crucial but distinct contributions to inhibitor binding with HCV NS3/4A. The structural basis of the potency has been elucidated, demonstrating the importance of the R155 sidechain conformation. This extensive exploration of binding energies and interactions between these compounds and HCV NS3/4A at the atomic level should benefit future antiviral drug design.
Nanosilibinin ameliorates anxiety, learning impairment and Wnt-β catenin related genes expression deficits in zebrafish model of Autism Spectrum Disorder
Authors: Karimi Zahra , Zarifkar Asadollah , Dianatpour Mehdi , Mirzaei Esmaeil , Dara Mahintaj , Aligholi Hadi 
Published: August 2022

Abstract
purposeThe present study evaluated the effect of Nanosilibinin (as an inhibitor of Wnt signaling pathway), on neurobehavioral and molecular deficits in Valproic acid zebrafish model of Autism Spectrum Disorder. MethodsZebrafish embryos were exposed to Valproic acid (1µM) and Nanosilibinin (100,200 and 500 µM) for 96 h, then survival rate, inattentive and anxiety behavior and the expression of CHD8, CTNNB, GSK3β, LRP6, TNFα, IL1β and BDNF genes were assessed 7 days post fertilization (dpf).ResultsThe results indicated that except 500 µM, other concentrations of Nanosilibinin didn’t have any adverse effect on survival, hatching and morphological development when were used with Valproic acid at the same time. In addition, 100 and 200 µM of Nanosilibinin could ameliorate the anxiety and learning deficit in zebrafish larvae. Real-time analysis revealed that Nanosilibinin prevented raising the expression of a number of genes associated with autism such as CHD8, CTNNB, GSK3 β, LRP6, TNFα, IL1β and BDNF after exposure to Valproic acid. ConclusionIn conclusion, Nanosilibinin treatment for the first 96 h of life showed therapeutic effect on an ASD-like phenotype by decreasing anxiety and learning deficits and reduction in expression of number ASD related genes.
Magnetic Fe 3 O 4 @polydopamine nanoparticle as an efficient adsorbent for simultaneous removal of Basic blue and Toluidine blue O dyes using derivative spectrophotometric determination and central composite design optimization
Authors: Zolgharnein Javad , Feshki Shahab , Rastgordani Mahdieh 
Published: August 2022

Abstract
Simultaneous determination and removal of Toluidine blue (TO) and basic blue (BB) dyes by dopamine-modified magnetic nanoparticles was considered. Nanomagnetic oxide coated with polydopamine (PDA) acid is introduced as a new and efficient adsorbent to remove cationic dyes in a binary mixture solution. Characterization of the Fe3O4 and Fe3O4@PDA nanoparticles was also supported by FT-IR, XRD, SEM, and VSM analysis. First-order derivative spectrophotometry is employed to simultaneously determine TO and BB in a binary mixture. Linear range for TO and BB in binary mixture obtained by y = 0.00014x + 0.00013 and y = − 0.00136x- 0.00487 least square equations was 2–20 mg L− 1 and limit of detection 0.4 and 0.3 mg L− 1, respectively. The effect of independent factors such as pH, dye’s initial concentration (C0), and adsorbent amount (m) on dye’s adsorption process was investigated. The response surface methodology was employed by running a central composite design (CCD) to obtain optimal values for dye removal percent. It leads to optimum conditions as follows: pH = 10˛ m = 25 (mg), CTO=320 (mg L− 1), CBB=350 (mgL− 1), %RTO=85, %RBB=80. Finally, isotherms, kinetics, and thermodynamics of the removal processes were also evaluated in the binary mixtures.
Infection of SARS-COV2 as a trigger for IgA-associated vasculitis.
Authors: Machura Edyta , Krakowczyk Helena , Bąk-Drabik Katarzyna , Szczepańska Maria 
Published: August 2022

Abstract
Background: IgA-associated vasculitis (IgAV), formerly known as Henoch-Schönlein Purpura-(HSP) disease, is the most common systemic vasculitis of developmental age. Approximately in 50% of the patients with HSP the outbreak of the disease, has been reported to be associated with streptococci, adenowirus, parvovirus, mycoplasma, respiratory syncytial virus (RSV), and influenza infection. Emerging reports in both adults and children has described few cases COVID-19 infection associated with HSP.Case presentation: We present the case of 7-year-old girl, was diagnosed with HSP, fulfilling 3 clinical criteria (palpable purpura and abdominal pain, arthralgia and edema), without renal involvement at which the infection with SARS-COV2 was confirmed by the presence of IgM and IgG antibodies. In the girl, disclosure of the HSP was preceded by a mild infection of the upper respiratory tract, treated symptomatically. During hospitalization we observed high values of inflammation markers, such as leukocytosis, increased neutrophil count and high NLR which are markers associated with IgAV gastrointestinal bleeding, which was also observed in the girl associated with rotavirus diarrheaConclusions: The case presented by us and by other authors indicate a possible role of SARS-CoV-2 in the development of HSP, but this requires further research.
Characterization of the wild plants in Wadi Degla Protectorate, North Eastern Desert, Egypt
Authors: Alhobishi Haifa A. , Slima Dalia F. , Turki Zaki A. , Shaltout Kamal H. 
Published: August 2022

Abstract
Wadi Degla protectorate is located between latitudes 29° 51′ 51″ N to 30° 00′ 34″ N and longitudes 31° 16′ 03″ E to 31° 39′ 11 E and its length reached to 30 km. It passes through the limestone rocks of the Eastern Desert. The present study aimed to evaluate the floristic characteristics of the wild plants in Wadi Degla protectorate. Including taxonomic diversity, life and sex forms, dispersal types, economic potential, threats and national and global floristic distributions. Field visits were conducted to 185 locations in Wadi Degla. From each location, plant and seed specimens were collected. One hundred and sixty one plants belonged to 128 genera and 43 family were recorded. They parts of the wadi (up, mid and down-stream). Therophytes were the most represented life form. Bisexuals were the most represented sex form. Sarcochores were the most represented dispersal type, followed by Desmochores. Small geographic range - national habitat - non abundant plants were the most represented rarity form. For national scales, Mediterranean and Sinai regions were the most rich wild plants. For global scales, Saharo-Sindian and Mediterranean were the most represented elements. Medicinal plants were the most represented good, while solid wastes were the most represented threat.
Response of plant-soil-microbe C–N–P stoichiometry to recovery succession in Sophora moorcroftiana shrubs in the middle reaches of the Yarlung Zangbo River
Authors: Dong Ruizhen , Yang Shihai , Wang Xiaoli , Xie LeLe , Ma Yushou , Wang Yanlong , Zhang Litian , Zhang Min , Qin Jinping 
Published: August 2022

Abstract
Background and aims Ecological stoichiometry in plants and soils plays a crucial role in biogeochemical cycles, energy flow and functioning in ecosystems. However, the stoichiometric responses of plants and soils along successional stages in alpine desert areas remain unclear.Methods We illustrated the C:N:P stoichiometry interactions among plants (green leaves, understorey herbs, and litter), soils, and soil microbes across three successional stages (moving dune, MD; semifixed dune, SFD; and fixed dune, FD) of the Sophora moorcroftiana shrub community. Results Recovery succession increased the C:N, C:P, N:P ratios of soils, but decreased the plants. Importantly, among the stoichiometric ratios of soil resources, the C:N ratio decreased with recovery succession. Leaf C:P and N:P ratios in S. moorcroftiana were higher than those in the understorey herb biomass, as P concentrations gradually decreased in leaves. Leaf N:P ratio increased with succession and varied from 15.83 to 28.26, suggesting that P was the more restricted element for the growth of the S. moorcroftiana population. Furthermore, significant increases in plant and soil C:N and C:P ratios and decreases in soil N:P ratios were observed with shrub age, suggesting that the demand for P became increasingly critical. Conclusion the stoichiometric ratios of plants and soils formed a complex network. Succession can balance plant and soil C:N:P ratios relationship dependent on the stoichiometry ratios of litter and microbial biomass. These findings are helpful for understanding the nutrient stoichiometry patterns and utilization strategies of C, N and P and scrubland restoration and management in alpine valley sand ecosystems.
Molecular identification of vivax malaria relapse patients in Yunnan Province based on the homology analysis of Plasmodium vivax circumsporozoite protein gene
Authors: Xu Yanchun , Dong Ying , Deng Yan , Huang Herong , Chen Mengni , Liu Yan , Wu Jing , Zhang Canglin , Zheng Webi 
Published: August 2022

Abstract
BackgroundMore than 85% of the malaria burden is caused by imported vivax malaria in Yunnan Province and Yunnan is also where the majority of vivax malaria patients are diagnosed across China. Timely removal of the source of Plasmodium vivax and its breeding environment remains the key to eliminating the secondary transmission of imported malaria. To compensate for the uncertainty of epidemiological surveys in tracing vivax malaria recurrence, this study attempted to use molecular markers for identification. Materials and methods To do so, blood samples were collected from cases diagnosed and revalidated as single infections of P. vivax in Yunnan Province from 2013 to 2020. Specifically, samples from suspected relapses with recurrent episodes were subjected to PCR amplification, product sequencing, and analysis of the P. vivax circumsporozoite protein (pvcsp) gene. ResultsSeventy-eight suspected recurrent patients were retrieved from 2484 vivax malaria cases, with a total of 81 recurrent episodes. A total of 159 blood samples from primary infection P. vivax and recurrences were subjected to PCR amplification and sequencing to obtain 156 CDS sequences of pvcsp gene, 121 of which can be matched into the paired sequences of 59 patients. There were 475 polymorphic loci and 84 haplotypes (H01-H84) in the 121 sequences. Also, there were 79 and 5 haplotypes with CRR repeat units (PRM) of VK210 and VK247 structure, respectively. Of the 59 pairs of pvcsp gene sequences, every one of 31 pairs showed only one haplotype and no variant sites, meaning the every paired sequences were completely homologous and the paired P. vivax strains were homologous single clone. Every one of the remaining 28 paired sequences had two haplotypes but no length polymorphism, and except for 2 polymorphic loci (39 and 1027), all single nucleotide polymorphisms were double-equivalent bases differentially transferred between paired sequences, indicating that the paired sequences are "weakly heterologous" with no fragment insertions (or deletions) and only individual site polymorphisms. All 59 vivax malaria recurrences were respectively caused by the activation of P. vivax hypnozoites from the same population as the primary infection. Conclusions The paired analysis of the similarity of Plasmodium high variant genes allowed the identification of recurrent episodes caused by P. vivax homologous hypnozoites, and also demonstrated pvcsp gene as one of the candidate molecular markers. Moreover, the study showed most of the hypnozoites causing vivax malaria recurrence in Yunnan Province belonged to homologous single clone or sibling strains comparison with the original infection strains.
A comparison between centrally and systemically administered erythropoietin on kidney protection in a model of fixed-volume hemorrhagic shock in male rats
Authors: Ranjbaran Mina , Kadkhodaee Mehri , Adelipour Maryam , Hafazeh Leila , Lorian Keivan , Seifi Behjat 
Published: August 2022

Abstract
In this study, a comparison between centrally and systemically administered erythropoietin (EPO) was performed on some kidney indices during hemorrhagic shock (HS) in male rats. Male Wistar rats were allocated into four experimental groups. 1) Sham; a guide cannula was inserted into the left lateral ventricle by stereotaxic surgery and other cannulas were placed into the left femoral artery and vein. 2) HS; stereotaxic surgery was done to insert a cannula in left lateral ventricle and after a 7-day recovery, hemorrhagic shock was induced by withdrawing fifty percent of blood volume and then resuscitation was performed with a mixture of Ringer’s lactate and the shed blood. 3) EPO-systemic; the procedure was the same as HS group except that animals received 300 IU/kg erythropoietin into the femoral vein immediately before resuscitation. 4) EPO-central; animals was treated to erythropoietin (2 IU/rat) into the left lateral ventricle before resuscitation. Arterial oxygen saturation (SaO2) was measured during experiments. Urine was collected for biochemical studies and renal tissue samples were stored for ex-vivo indices assessments. Erythropoietin (systemically/centrally administered) significantly improved SaO2, renal functional and oxidative stress parameters and decreased renal inflammatory (TNF-α and IL-6) mRNA expression compared to the HS group. EPO-treated groups showed a decrease in active form of caspase-3 protein level and an increase in autophagy activity in comparison to the HS group. Considering the fact that the effective dose of systemic EPO (300 IU/kg) was roughly 50 times higher than that of central administration (2 IU/rat), centrally administered EPO was accompanied with more advantageous consequences than systemic way. EPO is likely to act as a neuro-modulator or neuro-mediator in the central protection of organs including the kidneys.
A Rapid Solo Software Development (RSSD) Methodology based on Agile
Authors: Purba Kristo Radion , Ramli Rusyaizila 
Published: August 2022

Abstract
Existing software development methodologies were mostly focused on team-based development. Solo development presents its own challenges due to the lack of collaboration and resources. Existing solo development methodologies lacked efficiency, modularity, and revisitability, which become the values for the proposed RSSD (Rapid Solo Software Development) methodology. RSSD adopts the same main phases from the Agile methodology (meet, plan, design, develop, test, and evaluate), but with different subphases, values, and optimization measures. The efficiency value focuses on streamlining the planning process, including code structure planning and optimization strategies. Modularity focuses on dividing the codes into smaller functions and reducing dependencies. Lastly, revisitability focuses on improving the clarity of the diagrams, documentation, and code structure for easy revisits. The RSSD methodology was tested on 10 total projects, and respondents agreed that it was helpful to streamline the development process, with an average score of 4.19 out of 5.0. This study contributes to making a streamlined yet effective methodology that helps solo software developers to plan a project in a more structured manner.