bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA-Seq reveals molecular strategies in response to chronic hypoxic challenge in the gills of Takifugu rubripes
Authors: Lu Yun , Shang Fengqin , Li Yan , Han Bing , Wei Renjie , Liu Shengmei , Liu Ying , Liu Yang , Wang Xiuli 
Published: November 2022

Abstract
Hypoxia negatively affects fish behavior, growth, physiological metabolism, and immune responses, or even death. Due to the high density of breeding, sudden power outages and hypoxia stresses have become a serious problem for the aquaculture of Takifugu rubripes. As fish gills are the first organ to be affected by hypoxia in the aquatic environment, the ability to sense and respond to hypoxic stress is crucial. At the molecular level, therefore, the regulation of gene expression in the gills is essential for hypoxia tolerance. Using an RNA-Seq approach in the Illumina sequencing platform, we investigated transcriptome modulation in the gills of T. rubripes, in response to chronic hypoxic stress (2.5 ± 0.5 mg/L for 10 days). Transcriptomic analysis showed that 143 differentially expressed genes (DEGs) (|log2foldchange| >=1 & p.adj < 0.05) were detected under chronic hypoxic exposure (84 up-regulated and 59 down-regulated genes). According to GO annotation, KEGG, and GSEA enrichment analysis, we found that chronic hypoxia can cause damage to animal tissues. However, the functional analysis also showed that T. rubripes can improve the ability to resist hypoxic stress by changing the expression of genes related to metabolic processes and O2 extraction. Of particular interest was the activated immune-related pathways, including Cytokine-cytokine receptor interaction and CCR chemokine receptor binding, which play an essential role in the adaptation of T. rubripes to chronic hypoxic stress. Therefore, the regulated genes and pathways identified in this study enrich the molecular regulatory mechanism of T. rubripes in different hypoxic conditions, which is valuable for further studies regarding hypoxia tolerance in T. rubripes and other teleost fish, and pave the way for future research to understand the tissue-specific molecular responses to hypoxic stress.
Spatio-temporal Study of Pollutants in the Rio De Ondas Hydrographic Basin, West of Bahia, Brazil Using Multivariate Analysis
Authors: Rego Enoc Lima , Portela Joelma Ferreira , Ribeiro Camila Lima , Souza João Pedro Rudrigues , Tonhá Myller de Souza , Silva José Domingos Santos , Souza Jurandir Rodrigues 
Published: November 2022

Abstract
Water bodies are containers that receive a large load of pollutants through the release of domestic, industrial, and agricultural effluents. With this focus, this work aimed to conduct a temporal-spatial variability study in the Rio de Ondas Hydrographic Basin through multivariate statistical analysis. For this, seventeen collection sites were established in four stations along the Rio de Ondas and its tributaries between 2017 and 2018. Ionic chromatography with suppressed conductivity was used for ions determination, while ICP-OES determined metals' total concentrations. The land use and occupation assessment between 1985 and 2015 was conducted using images from Landsat 5 and 8 satellites and the descriptive and multivariate analysis of the data using version 10 of the Statistica software. The results showed that, in thirty years, there was a growth of 569% of agricultural activities in the watershed area, with significant suppression of native vegetation, favoring the transport of pollutants to rivers. Ca2+, PO42−, Al, Cu, and Zn concentrations showed a statistically significant difference between the seasons, with higher medians in the rainy season. Rainy season influenced the formation of three groups in the PCA, consisting of electrical conductivity, salinity, TDS, and PO42− (group 1); temperature, Fe, SO42− and Cl− (group 2); and Ca2+, Mg2+, Na+, and HCO3− (group 3). The strong correlation between parameters of each group indicates anthropic influence on the watershed's water quality. However, levels are within the potability standard.
Self-care and Health-related Quality of Life among Heart Failure patients in Jimma Medical Center, Southwest Ethiopia
Authors: Seid Sheka Shemsi , Ibro Shemsedin Amme , Senbiro Ismael Ahmed , Ahmed Abdulwahid Awol 
Published: November 2022

Abstract
Background:Understanding self-care behaviour and its relationship with health-related quality of life (HRQoL) in patients with Heart failure help a healthcare provider to identify patients who still need but lack the self-care skills required to manage their disease condition at home. However, despite its growing importance and obvious relationships with positive health outcomes,the evidence to justify its popularity is not well documented. This study aimed to assess the extent of heart failure self-care and its relationship with the HRQoL among patients with heart failure.
Methods: An institutional-based descriptive correlational study was conducted on 176 heart failure patients at Jimma Medical Canter. The data were analyzed using SPSS version 26. Both descriptive and analytical statistical tests were utilized. A multivariate linear regression analysis was done to determine the relationship between self-care behaviour and HRQOL and different independent variables. Variables with a p-value< 0.05 were considered as significant predictors of the outcome variable.
Results:Of all participants, the majority, 93(52.8%) were males. The average self-care scores for maintenance, management, and confidence were 44.35 ±16.75, 43.81±19.15, and 50.16±24.17, respectively. None of the self-care dimensions reached the self-care adequacy cut point of 70. The majority of the study participants 111(63.07%) had poor quality of life. Significant linear associations were observed between self-care management and overall HRQoL (βs=-1.26, p=.000) and physical dimension (βs=0.881, p=.000). Self-care confidence score was also significantly associated with total HRQoL(βs=-.579, P= 0.029), physical(βs=.507, p=.007) and emotional dimensions score(βs=-.299, p=0.038).
Conclusions: The study participants had inadequate self-care behaviour and poor health-related quality of life. HF self-care was associated with better health-related quality of life. Therefore, the healthcare provider should identify patients with heart failure who lack self-care behaviours required to manage their condition at home and focus should also be given to specific educational interventions that target determinants of self-care behaviour and quality of life.
Proteomic analysis of circulating small extracellular vesicles unique to cervical cancer
Authors: Molika Piyatida , Leetanaporn Kittinun , Rungkamoltip Petchploy , Roytrakul Sittiruk , Hanpresertpong Jitti , Navakanitworakul Raphatphorn 
Published: November 2022

AbstractBackground Small extracellular vesicles (sEVs) are membrane vesicles released by healthy and malignant cells. sEVs are potential biomarkers for cancer diagnosis. Cervical cancer (CC) is the fourth most common cancer in females worldwide. Existing biomarkers, such as squamous cell carcinoma antigens, show low specificity. Hence, a novel biomarker for the diagnosis of CC is required. This study aimed to identify potential candidates in sEVs through proteomic analysis for the diagnosis of CC and to determine the EV protein profile to distinguish between healthy and CC serum samples. Methods The number and size distribution of sEVs in healthy controls (HC) and CC were measured using nanoparticle tracking analysis. Differential ultracentrifugation combined with size-exclusion chromatography was used to isolate and purify sEVs derived from the serum of HC and CC. The isolated sEVs were characterized using western blotting and transmission electron microscopy. Liquid chromatography-tandem mass spectrometry was used to identify and compare the protein profiles between CC and HC. EV proteins were validated using the TCGA database. Results The particle concentration in CC was marginally higher than that in HC. The mode size of the particles in CC was significantly smaller than that in the HC-derived particles. Proteomic and functional protein analyses revealed a difference in the EV protein profiles between HC and CC. We found three and 18 uniquely expressed proteins in HC and CC, respectively. Unique EV proteins in CC are involved in angiogenesis and the Ras, VEGF, and FAS signaling pathways, while EV proteins in HC are involved in cellular homeostasis. EV proteins such as C1QB, MYO3B, and NADSYN1 were significantly upregulated in CC and primary tumor tissues, whereas MAFK, OR13C9, PIK3C2, PLCB4, RAB12, and VIP were downregulated in CC sEVs and primary tumor tissues. Conclusion Our study provides useful insights into the potential of sEVs as noninvasive biomarkers for CC diagnosis. Validation with a well-designed cohort should be performed to assure the clinical diagnostic value of specific protein markers for CC sEVs.
Effects of Distinct Dual-tasks on Gait and the Correlation between Gait speed and Clinical Features in Parkinson’s disease
Authors: Zhang Meimei , Gan Yawen , Wang Xuemei , Wang Zhan , Zhang Yumei 
Published: November 2022

Abstract
Background Gait impairment is a common and disabling motor symptom in Parkinson’s disease (PD), deteriorated gait parameters have showed in both single-task (ST) and dual-task (DT) conditions. The aim of this study was to investigate the effects of different motor-cognitive and motor-motor DTs on gait and the correlation between gait speed and clinical features in PD patients.
Methods Fifty-six individuals with PD completed two motor-cognitive DTs (serial-7 subtractionand digit backward) and one motor-motor DT (button pressing). Spatiotemporal gait parameters were evaluated by wearable sensors. DT effects (DTEs) of gait parameters were calculated. Clinical variables recorded including Hoehn & Yahr (H-Y) staging, Movement Disorder Society Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) part I, II and III, New Freezing of Gait Questionnaire (NFOG-Q), Montreal Cognitive Assessment (MoCA), Hamilton Anxiety Rating Scale (HAMA), Hamilton Depression Rating Scale (HAMD), 39-item Parkinson’s Disease Questionnaire (PDQ-39) and Nonmotor Symptom Scale (NMSS).
Results Gait parameters including gait speed, cadence, stride length, gait cycle duration, double support phase deteriorated under the motor-cognitive DT conditions by Paired-sample t test and Wilcoxon signed-rank test (p＜0.01, p＜0.05). The motor-motor DT had no significant effect on gait performance except for gait speed (p＞0.05). The serial-7 subtraction DT paradigm had similar effect on gait with the digit backward DT. Gait speed was negatively correlated with MDS-UPDRS I, II, HAMA, HAMD, NMSS and PDQ-39 scores in PD patients under both ST and DT conditions (p＜0.01, p＜0.05).
Conclusion Effects of DT conditions on gait deficits were independent of the types of cognitive tasks. Gait speed was influenced by clinical features of PD under both ST and DT conditions. Whatever the types, motor-cognitive DT training should be used to improve gait performance under DT conditions, which is required to provide more therapeutic support of PD patients in the future.
Efficacy of futibatinib, an irreversible fibroblast growth factor receptor inhibitor, in FGFR-altered breast cancer
Authors: Meric-Bernstam Funda , Saridogan Turcin , Akcakanat Argun , Zhao Ming , Evans Kurt , Yuca Erkan , Scott Stephen , Kirby Bryce , Zheng Xiaofeng , Ha Min Jin , Chen Huiqin , Ng Patrick , DiPeri Tiimothy , Mills Gordon , Rodon Jordi , Damodaran Senthil 
Published: November 2022

Abstract

The role of the fibroblast growth factor receptor (FGFR) gene alterations as therapeutic targets in breast cancer have not been well characterized. Futibatinib (TAS-120; Taiho) is a novel pan-FGFR inhibitor. We sought to determine the efficacy of futibatinib in breast cancer models withFGFRalterations. Nine breast cancer patient–derived xenografts (PDXs) with a variety ofFGFR1-4 alterations and expression levels were treated with futibatinib. FGFR gene expression between patient tumors and matching PDXs was significantly correlated. Futibatinib inhibited tumor growth in 3 of 9 PDXs, with tumor stabilization in anFGFR2-amplified model and prolonged regression in an FGFR2 Y375C mutant/amplified model. FGFR2 overexpression and, to a greater extent, FGFR2 Y375C expression in MCF10A cells enhanced cell growth and sensitivity to futibatinib. Per institutional and public databases,FGFR2mutations and amplifications had a population frequency of 1.1–2.6% and 1.5–2.5%, respectively.FGFR2alterations in breast cancer may represent infrequent but highly promising targets for futibatinib.
Sleep duration and consumption of ultra-processed foods among Brazilian adolescents
Authors: Rocha Luana , Inácio Monique , Gratão Lucia , Miquelanti Isabella , Andaki Gustavo , Cunha Cristiane , Oliveira Tatiana , Pessoa Milene , Mendes Larissa 
Published: November 2022

Abstract

Background: Sleep duration influences adolescents eating patterns and quality of life. Thus, the study objective was to verify the association between sleep duration and ultra-processed food (UPF) intake among Brazilian adolescents participating in the Study of Cardiovascular Risks in Adolescents (ERICA).
Methods: Cross-sectional study using ERICA data, conducted between March 2013 and December 2014 with adolescents aged 12 to 17 years from public and private schools. UPF intake was obtained by recording a 24-hour food diary, and sleep duration through a questionnaire, stratified into: recommended sleep duration (from 8 to 10 hours) and inadequate sleep duration (less than 8 hours and more than 10 hours). Linear regression was performed to verify the association between sleep duration and UPF consumption.
Results: 66,791 adolescents were evaluated. Adolescents with inadequate sleep duration showed an increase in UPF consumption.
Conclusion: This result can contribute to the development of multifactorial interventions to improve adolescents’ quality of life, emphasizing the importance of adopting healthy lifestyle habits.
Validity and reliability of the Swedish versions of the HLS-EU-Q16 and HLS-EU-Q6 questionnaires
Authors: Bergman Lina , Nilsson Ulrica , Dahlberg Karuna , Jaensson Maria , Wångdahl Josefin 
Published: November 2022

Abstract
Background: In Europe at least one in every three people has low health literacy. The Health Literacy Survey European Questionnaire (HLS-EU-Q16) has been adapted and translated into Swedish but not psychometrically evaluated. The Swedish HLS-EU-Q6 has not been used in any study nor has it been validated. We aimed to study the psychometric properties of the Swedish versions of the HLS-EU-Q16 and HLS-EU-Q6.
Methods: A psychometric study of Swedish-speaking adults ≥ 18 years (n = 347). Statistical analysis was guided by The COnsensus-based Standards for the selection of health Measurement Instruments and included item distributional statistics, construct and criterion validity, test-retest reliability and internal consistency.
Results: Swedish HLS-EU-Q16: There were no floor effects; a ceiling effect was noted in 28% of respondents. Construct validity showed correlations with education level (rho = 0.135), self-perceived health (rho = 0.167), electronic health literacy (rho = 0.496), age (rho = 0.71), and Swedish HLS-EU-Q6 (rho = 0.840). Test-retest showed Cohen’s κ = 0.822. For internal consistency, Cronbach’s α = 0.89, and the split-half reliability = 0.93. Swedish HLS-EU-Q6: No floor or ceiling effects were noted. Construct validity showed correlations with education level (rho = 0.157), self-perceived health (rho = 0.177), age (rho = 0.400), electronic health literacy (rho = 0.551), and the Swedish HLS-EU-Q16 (rho = 0.840). The agreement between the Swedish HLS-EU-Q16 and Swedish HLS-EU-Q6 was poor, Cohen’s κ = 0.43. Test-retest showed Cohen’s κ = 0.812. For internal consistency, Cronbach’s α = 0.77, and the split-half reliability = 0.80.
Conclusions: The Swedish version of the HLS-EU-Q16 has acceptable psychometric properties, and based on the results we recommend its use to measure health literacy. Our results did not demonstrate the validity of the Swedish version of HLS-EU-Q6 because the agreement with the Swedish HLS-EU-Q16 was poor.
Cryptic colonization and transmission of mcr-1-positive Escherichia coli in mouse intestines
Authors: Shen Yingbo , Lv Ziquan , Chen Kun , Liu Weiwen , Xiang Qiumei , Jiang Junyao , Shao Dongyan , Liu Dejun , Zhang Rong , Wu Congming , Wang Yang , Ke Yuebin , Shen Jianzhong 
Published: November 2022

Abstract
Background Mobile colistin-resistance gene mcr-1 is prevalent among various bacteria, hosts and countries, especially in the guts of humans and animals. However, the biological basis for mcr-1 colonization and transmission in the intestines is largely unknown.
Methods We used mouse models to mimic exposure to mcr-1-positive Escherichia coli (MCRPEC, harboring an IncI2 plasmid positive for mcr-1) in human intestines without and with antibiotic pretreatment, respectively. We used cultivation and qPCR method to determine the presence and quantity of MCRPEC. We also used Fluorescence in situ hybridization (FISH) method to locate mcr-1-positive bacteria in situ and confocal laser scanning microscopy method to examine the interaction between MCRPEC and Caco-2 cell in vitro. Finally, we used mouse model to investigate the transmission of MCRPEC among individuals.
Results We found two approaches of MCRPEC colonization in the mouse intestines after exposure. In mice with intestinal microbiota homeostasis, MCRPEC was transient in the large intestines, while mcr-1 was detected at lower abundances (10-4–10-5) for at least 21 days as relic DNA or mcr-1-positive uncommon bacteria (MCRPUB). In mice with intestinal microbiota dysbiosis, MCRPEC colonized the intestines directly with a high shedding load (~108 CFU/g feces) and high mcr-1 abundance (~10-2). The transmission model confirmed that both MCRPEC and MCRPUB could cryptically transfer among individuals and persist for long periods.
Conclusions These results demonstrate two approaches of MCRPEC colonization and a long persistence of MCRPUB or an antetype in mouse intestines as well as their transmission among mice, which partially explains the widespread prevalence of mcr-1-positive IncI2 plasmid among hosts and its long persistence in the gut even without antibiotic pressure. Contaminated food enables exposure to pathogens that can colonize human intestines; thus, reducing MCRPEC/MCRPUB in livestock and animal-derived food and preventing MCRPEC/MCRPUB transmission in ecosystems under the "One Health" perspective are crucial.
Distribution, local use, and bio-prospecting opportunity of Ocimum americanum L around North Western part of Amhara Region, Ethiopia
Authors: Merawi Edeget , Birhanu Taye 
Published: November 2022

Abstract
Ocimum americanum encompasses various uses. The plant is claimed as a food additive for its aromatic effect on different foods and folk medicine for various complaints. However, the plant genetic resource use category, distribution, and associated community knowledge have not been properly and systematically documented and utilized. Accordingly, this survey was performed to explore the bio-prospecting opportunity of Ocimum americanum, and its associated community knowledge to provide baseline data for bio-digging companies. Purposive and proportional samplings were jointly used to select districts, villages, users, and growers who participated in the study. Dembiya, Gonder Zuria, Takusa, Alefa, Bahir Dar city, and Bahir Dar Zuria districts were selected based on the growth potential and user availability. Data was collected through semi-structured interviews, group discussions, and field observation. The community used Ocimum americanum for various purposes; for ailment treatment (31.67%), food flavor (26.6%), food preservative (7.7%), and to give aroma to different foods (8.33%). 31.67 % of the respondents replied as they are a user group of the plant for its ethno medicinal purpose whereas the remaining 68.34% of them claimed as they are not used for medical purposes. They are used to treat majorly for depression/headaches, and evil spirits. Concoction (21.05 %), cutting and inserting whole plant (15.79%), grinding (15.79%), melting (5.26%), Fumigation (10.53%), and smelling (31.58%) were used as methods of preparation and administration. Medicinal dosage is not quantized and varied up to the users based on the intensity and the severity of the disease. A total of nine typical basil-spiced traditional foods were listed. The highest fidelity level [a particular use category] of basil was food flavor (18.33%) followed by a similar result [18.33%] for Food flavor/preservative. The output of the direct matrix analysis showed Chilli pepper spice was the preferred local food sweetened by basil for its various purposes by the local people followed by hot-spiced pepper. Therefore, the present survey, inferred the community traditionally uses basil for its aromatic, preservative, flavor, and ailment treatment role. Therefore, novel genetic resources should be further researched and promoted for versatile use. It gives a clue for bio-prospecting companies working in the food and pharmacological industries to access the associated local knowledge and practices documented as well as the plant genetic resource to search for new biochemical compounds responsible for medicinal, preservative, and aromatic effects.
The Application of Rapid Response Team in Category 1 Emergency Caesarean Section Teaching for OBGYN Residents in the Delivery Room
Authors: Xu Xia , Lin Ying , Weng Ling , Guo Yanni , Lin Lin , Yan Jianying 
Published: November 2022

Abstract
Background
Category 1 caesarean section (CS) can be a life-saving procedure when there is immediate threat to the life of the woman or fetus. However, category 1 CS is an challenge for OBGYN residents, and it is necessary to establish a effective and straightforward teaching strategy. This study aimed to evaluate the efficiency of rapid response team (RRT) oncategory 1 CS teachingfor OBGYN residents in the delivery room.
Methods
A total of 142 residents who underwent standardized residency training programs in the delivery room were divided into a RRT teaching group and a traditional response (TR) teaching group. In the RRT teaching group, Category 1 emergency CS teaching was started and explored by rapid response team. The training included both theoretical and practical components. After the training, decision-to-delivery interval (DDI) , neonatal Apgar score , operation time and rate of postpartum hemorrhage (PPH) were compared. A questionnaire on the subjective assessment of various aspects of the program was conducted at the end of the training period.
Results
The decision-to-delivery internal (DDI) in minutes in the RRT teaching group (n=72) was significantly shorter than that of the TR teaching group (n=70) (11.83±4.16 vs 13.56±5.47, p = 0.0364). The score of satisfaction from residents in the RRT teaching group was significantly higher than that of the TR group [7 (6, 9) vs 9 (7, 10), p =0.0154 ). Compared wtih the TR teaching group , more residents thought their clinical skills have been improved (94.29% vs 100%, p=0.0396) and willing to recommend their training method to others (91.43% vs 100%, p=0.0399) in the RRT teaching group . However, no significant differences were observed in the incidence of postpartum hemorrhage between the two groups.
Conclusions
RRT teaching is beneficial in the standardized training and teaching of residents in the delivery room. It improves the DDI of category 1 emergency caesarean section and the degree of satisfaction.