bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research trends of mesenchymal stem cells application in orthopedics: A bibliometric analysis of the past 2 decades
Authors: Deng Zhibo , Luo Fenqi , Lin Yuan , Luo Jun , Ke Dianshan , Song Chao , Xu Jie 
Published: September 2022

BackgroundBibliometric analysis and visualization tools were used to determine the development trend of mesenchymal stem cells (MSCs) in orthopedics in the past 20 years, so as to guide researchers to explore new directions and hotspots in the field in the future.MethodsIn the Web of Science Core Collection, all articles about the application of MSCs in orthopedics from 2002 to 2021 were searched. The qualitative and quantitative analysis was performed based on Web of Science and CiteSpace software.ResultsA total of 2,207 articles were retrieved. After excluding non-article articles such as review and letter and non-English language articles, 1,489 articles were finally included. Over the past 2 decades, the number of publications on the application of MSCs in orthopedic diseases increased. Among them, the United States, China, Japan and the United Kingdom have made significant contributions in this field. The most productive institution was Shanghai Jiao Tong University. Journal of Orthopedic Research published the largest number of publications. The journal with the highest citation frequency was Experimental Hematology. The authors with the highest output and the highest citation frequency on average were Rochy S. Tuan and Scott A. Rodeo, respectively. “Mesenchymal stem cell”, “in vitro” and “Differentiation” were the top three keywords that appeared. From the keyword analysis, the current research trend indicates that the primary research hotspots of MSCs in orthopedics are the source of MSCs, in vitro experiments and the differentiation of MSCs into bone and cartilage. The frontiers of this field are the combination of MSCs and platelet-rich plasma (PRP), the treatment of knee diseases such as osteoarthritis, osteogenic differentiation, and the application of biological scaffolds combined with MSCs.ConclusionOver the past 2 decades, the application of MSCs in orthopedic diseases has received increasing attention. Our bibliometric analysis results provide valuable information and research trends for researchers in the field to understand the basic knowledge of the field, identify current research hotspots, potential collaborators, and future research frontiers.
Empyema caused by Eikenella halliae diagnosed by metagenomic next-generation sequencing (mNGS) after pulmonary surgery: A case report
Authors: Huang Jie , Wang Liming , Xu Yumei , Hu Xinhua , Yu Ronghuan , Chen Shi , Wang Baoqing 
Published: September 2022

BackgroundEmpyema is one of the complications of pulmonary surgery for lung cancer, the incidence of which is not very high, but in severe cases, it can even lead to death, and it is always difficult to diagnose the cause by conventional methods.Case presentationIn this study, we report a clinical case of empyema caused by Eikenella halliae after pulmonary surgery in a 55-year-old man. He had a fever, cough, and expectoration for 3 days and was diagnosed with right hydropneumothorax and empyema, pneumonia, postoperative malignant tumor of the right lower lobe (adenocarcinoma), and hypertension. The microbiology laboratory reported Gram-negative bacteria in pleural effusion, which was preliminarily considered as Eikenella based on culture and 16S rRNA sequencing. Furthermore, metagenomic next-generation sequencing (mNGS) of sputum samples was performed two times and reported negative results and the presence of E. halliae, respectively. The pathogen was finally confirmed as E. halliae by whole genome sequencing, suggesting the high-resolution ability of mNGS in the clinical diagnosis of this case.ConclusionTo our knowledge, this is the first case report of E. halliae infection in China, indicating increased pathogenicity of Eikenella sp. in immunocompromised patients, especially after invasive operations. Our findings emphasize that mNGS allows bacterial diagnosis of empyema and can significantly improve the accuracy of the diagnosis.
Gastrointestinal problem among Indian adults: Evidence from longitudinal aging study in India 2017–18
Authors: Dawoodi Sameer , Dawoodi Inshiya , Dixit Priyanka 
Published: September 2022

IntroductionDiseases and illnesses of the gastrointestinal system (GIS) have grown in the last decade due to considerable lifestyle changes. People with gastrointestinal (GI) diseases have a high prevalence of depression, stress, anxiety, and impaired central nervous system functioning. Therefore, this study aims to explore the factors associated with the self-reported gastrointestinal problems among the Indian elderly and to explore the relationship between non-communicable diseases (NCDs), such as hypertension, heart diseases, diabetes, and neurological or psychiatric and gastrointestinal disorder.MethodsThis study uses data from the Longitudinal Aging Study in India (LASI), a population-based national survey, conducted during 2017–2018 with a representative sample of 72,250 individuals. Descriptive statistics were used to provide the frequency distribution of sociodemographic and economic profiles of adults. Bivariate analysis was used to understand the percentage distribution of adults suffering from gastrointestinal problems by their background characteristics. Binary logistic regression was used to determine the factors associated with gastrointestinal problems. In the binary logistic regression analysis, a systematic model building procedure was adopted.ResultsThe overall prevalence of self-reported gastrointestinal problems was 18%, with significant variations among regions, and it substantially increased with the increasing age of men. Hypertension and neurological problems have significant individual effects on gastrointestinal problems. Prevalence was higher in those who suffered from neurological or psychiatric problems (27%) than in those who suffered from hypertension (22%) and heart disease (23%). Adults from the age group 45–54 (1.11, p &lt; 0.01) and 55–64 (1.09, p &lt; 0.01) years were significantly more likely to have gastrointestinal problems compared with the &lt;44 years age group. Former and current smokers and adults with the habits of chewing tobacco were significantly more likely to report gastrointestinal problems than their counterparts. Moreover, the increasing economic status significantly and positively increased the likelihood of having self-reported gastrointestinal problems among adults.ConclusionAging-related gastrointestinal problems are physiological or pathological and more prevalent in the elderly population aged 64 years and above. Hence, policies and interventions have to be made age-specific. Gastrointestinal problems among older adults are acquiring greater importance in clinical practices to plan effective treatment, administration of gastrointestinal drugs, the early screening of gastrointestinal diseases. Given the policy focus through Health and Wellness centers for accessible NCD care, it is important that gastro-intestinal illnesses receive more focus and systemic support.
Assessment of COVID-19 prevention practice and associated factors in Jimma town, Ethiopia: A mixed study
Authors: Bedane Deriba , Abdissa Daba , Leta Bati , Gerema Urge , Lomboro Abraham , Kune Guta , Neme Abiru , Kene Kumsa , Berhanu Nimona , Dubiwak Abebe Dukessa , Tareke Kasahun Girma 
Published: September 2022

BackgroundCOVID-19 has affected the mental and physical wellbeing, social structure, countries' economy as well as individuals and community resilience, trust, and inequalities among societies. However, now almost all of the activities have been returned to the pre-corona era, despite the emergence of new strains and the spread of the disease. Hence, this study was conducted to assess COVID-19 prevention practice and the associated factors.Materials and methodsA community-based cross-sectional study triangulated with the qualitative findings was conducted in Jimma town, Oromia, Ethiopia. A total of 422 sample households were involved in the quantitative study. The quantitative data were collected using a structured questionnaire and 12 key informants were also interviewed for the qualitative part. The quantitative data were processed and entered into the Epi Data version 4.6 (software) and analyzed using SPSS 26.0. Similarly, the qualitative data were analyzed using ATLASti.7.1.04 software package. Descriptive statistics and binary logistics regression (p &lt; 0.25) were conducted to identify the candidate variable for multivariable logistics regression analysis (p &lt; 0.05) and a 95% confidence interval was used to establish the level of significance of the variables with the practice.ResultsInterviews were conducted with a total of 422 participants, yielding a response rate of 100%. Good preventive practices were found to be adopted by 13.3% of the respondents. People aged ≥ 50 years, [AOR = 2.85, 95%, CI = 1.246–0.53] who recovered from COVID-19, [AOR = 2.41, 95%, CI = 1.184–0.92], had chronic diseases [AOR = 3.70, 95%, CI = 1.887–0.25], and living with COVID-19 high risk [AOR = 2.96, 95%, CI = 1.475–0.991 were independently associated with good preventive practices.ConclusionIn this study, it was understood that there were poor COVID-19 preventive practices among the study participants. There was a disparity in adherence to the preventive practices in relation to (i.e., 50 and above years) the experience of contracting COVID-19 and people aged above 65 years old living with the high-risk group. In addition, the community had different misconceptions or risk perceptions related to COVID-19 infection and preventive practices. This highlights the need to design health education programs and implement risk and/or social and behavior change communication interventions to change perceptions or misconceptions of people or community members to bring about the desired behavioral change and prevent the spread of COVID-19.
Diagnostic ability and its influenced factors of ultrasound-guided percutaneous pleural needle biopsy diagnosis for malignant pleural mesothelioma
Authors: Zhang Yuxin , Tang Jiaxin , Zhou Xinghua , Chen Wuxi , Zhang Shiyu , Li Yuqin , Zhou Dazhi , He Liantu , Tang Qing 
Published: September 2022

BackgroundMalignant pleural mesothelioma (MPM) is a highly invasive malignant tumor. Ultrasound guidance has the advantages of real-time, convenience and nonradiative. We sought to identify diagnostic value and its influenced factors of ultrasound-guided percutaneous pleural needle biopsy (US-PPNB) for MPM.MethodsPatients who underwent US-PPNB between March 2014 and March 2020 and were finally diagnosed with MPM were retrospectively analyzed. We retrospectively analyzed the US-PPNBs pathological results of all patients clinically confirmed as MPM, and divided US-PPNBs into correctly and incorrectly diagnosed groups. Patient, thoracic, and biopsy variables that affected diagnostic accuracy were assessed. All variables significant on univariate analyses were subjected to multivariate logistic regression to identify significant predictors of diagnostic accuracy. We derived cutoffs for all significant continuous variables and used the Mantel–Haenszel test to determine whether the diagnostic accuracy of US-PPNB for MPM increased with pleural thickness.ResultsIn total, 49 patients with clinically confirmed MPM underwent US-PPNB; 37 diagnoses were correct and 12 were incorrect (accuracy = 75.5%). The pleura was significantly thicker in the correctly diagnosed group (p &lt; 0.001). The pleural thickness cutoff was 4.15 mm and diagnostic accuracy increased with pleural thickness grade (p for trend &lt;0.05). The diagnostic accuracy was significantly higher when 16-G rather than 18-G biopsy needles were used (p &lt; 0.05). Multivariate logistic regression showed that pleural thickness (odds ratio: 17.2, 95% confidence interval: 2.8–104.1, p = 0.002) and needle size (odds ratio: 6.8, 95% confidence interval: 1.0–44.5, p = 0.044) independently predicted diagnostic accuracy.ConclusionUS-PPNB afforded high MPM diagnostic accuracy, and pleural thickness and needle size significantly impacted accuracy.
A single-center comparison of our initial experiences in treating penile and urethral cancer with video-endoscopic inguinal lymphadenectomy (VEIL) and later experiences in melanoma cases
Authors: Gómez-Ferrer A. , Collado A. , Ramírez M. , Domínguez J. , Casanova J. , Mir C. , Wong A. , Marenco J. L. , Nagore E. , Soriano V. , Rubio-Briones J. 
Published: September 2022

BackgroundVideo-endoscopic inguinal lymphadenectomy (VEIL) is a minimally invasive approach that is increasingly indicated in oncological settings, with mounting evidence for its long-term oncological safety.ObjectivesTo present our single-center experience of treating penile and urethral cancer with VEIL, as well as its more recent application in melanoma patients.MethodsWe prospectively recorded our experiences with VEIL from September 2010 to July 2018, registering the patient primary indication, surgical details, complications, and follow-up.ResultsTwenty-nine patients were operated in one (24) or both (5) groins; 18 had penile cancer, 1 had urethral cancer, and 10 had melanoma. A mean 8.62 ± 4.45 lymph nodes were removed using VEIL and of these, an average of 1.00 ± 2.87 were metastatic; 16 patients developed lymphocele and 10 presented some degree of lymphedema; there were no skin or other major complications. The median follow-up was 19.35 months; there were 3 penile cancer patient recurrences in the VEIL-operated side. None of the melanoma patients presented a lymphatic inguinal recurrence.ConclusionsVEIL is a minimally invasive technique which appears to be oncologically safe showing fewer complications than open surgery. However, complications such as lymphorrhea, lymphocele, or lymphedema were not diminished by using VEIL.
Individualized 3D printed navigation template-assisted atlantoaxial pedicle screws vs. free-hand screws for the treatment of upper cervical fractures
Authors: Niu Guoqi , Cheng Jiawei , Liu Lutan , Li Chao , Zhou Gong , Chen Hui , Liu Tao , Nie Hu , Sun Zheng , Jiang Weili , Zhou Qiankun , Zhao Baoyin , Zhu Jun , Yu Ruochen , Guo Yalong , Yang Yi , Bai Jianzhong 
Published: September 2022

ObjectiveThis study aims to compare the efficacy and safety of freehand atlantoaxial pedicle screws against custom 3D printed navigation template screws in the treatment of upper cervical fractures.MethodsIn our institution from 2010 to 2020, a retrospective cohort analysis of 23 patients with upper cervical fractures was done. These patients were separated into two groups: group A (N = 12), which received customized 3D printed navigation template-assisted screws with virtual reality techniques, and group B (N = 11), which received freehand screws assisted by intraoperative fluoroscopy. Every patient was monitored for more than 1 year. The two groups were contrasted in terms of screw implant accuracy, cervical spine Japanese Orthopaedic Association (JOA) score, American Spinal Injury Association (ASIA) score, visual analogue scale (VAS) score, surgical time, fluoroscopy times, and intraoperative blood loss.ResultsA total of 88 atlantoaxial pedicle screws in all, 46 in group A and 42 in group B, were implanted. In group A, the screw insertion accuracy rate was 95.7%, compared to 80.0% in group B (P &lt; 0.05). When compared to group B, group A had shorter surgery times, less blood loss, fewer fluoroscopies, a higher short-term JOA score, and overt pain reduction (P &lt; 0.05). However, there was no discernible difference between the two groups' VAS scores, long-term JOA scores, or ASIA scores (sensory and motor), at the most recent follow-up.ConclusionIndividualized 3D printed guide leads to significant improvement in the screw safety, efficacy, and accuracy, which may be a promising strategy for the treatment of upper cervical fractures.
Occurrence of Nine Antibiotics in Different Kinds of Sewage Sludge, Soils, Corn and Grapes After Sludge Spreading
Authors: Barreiro Ana , Cela-Dablanca Raquel , Nebot Carolina , Rodríguez-López Lucía , Santás-Miguel Vanesa , Arias-Estévez Manuel , Fernández-Sanjurjo María , Núñez-Delgado Avelino , Álvarez-Rodríguez Esperanza 
Published: September 2022

The huge worldwide use of antibiotics triggers the accumulation of these substances in sludge from wastewater treatment plants (WWTP) and the possible contamination of soils amended with it, as well as of crops growing in these soils. In this work we analyzed the presence of the antibiotics amoxicillin (AMO), cefuroxime (CEF), ciprofloxacin (CIP), clarithromycin (CLA), levofloxacin (LEV), lincomycin (LIN), norfloxacin (NOR), sulfadiazine (SUL), and trimethoprim (TRI), in sludge from different WWTPs in Galicia (NW Spain), as well as in sludge technically treated by waste-managers, in soils where treated sludge was applied, and in crops (corn and vineyard) growing in the amended areas. The antibiotics were quantified by means of high resolution HPLC-mass-chromatography. The results indicate that almost all the sludge samples contained antibiotics, being ciprofloxacin and levofloxacin the most abundant reaching maximum values of 623 and 893 ng/g, respectively. The sludge treatment significantly reduced the number and the concentrations of antibiotics. In 12% of the soil samples where sludge was applied, some antibiotics were detected, but always in small concentrations. Regarding the crops, no antibiotic was detected in the roots, stalk, leaves and grain of corn, neither in grapes sampled in vineyards. It can be concluded that the treatments currently applied in the WWTPs under study are not totally effective in removing antibiotics from the sludge, although the findings of this research suggest that the additional specific treatment of the sludge derived from these WWTPs is effective in reducing the risk of environmental pollution due to a variety of antibiotics, and specifically in the case of soils amended with these organic materials and crops growing on it.
Prevalence of periodontal diseases in patients with different body mass index
Authors: Slazhneva E. S. , Atrushkevitch V. G. , Orekhova L. Yu. , Loboda E. S. 
Published: September 2022

Relevance. The impact of obesity, as a multifactorial and multigenic disorder, on human health is a complicated multidisciplinary and simultaneously relevant problem in modern society. Inflammatory periodontal diseases are among the multiple consequences of obesity, with adverse effects on the quality and duration of life. Aim: To analyze the prevalence of inflammatory periodontal diseases in patients with metabolic syndrome according to the body mass index (BMI).Material and Methods. We analyzed 306 records of patients with chronic inflammatory periodontal diseases. Patients’ weight and height were stated in the questionnaire attached to the dental patient record. The patients formed five groups based on their BMI.Results. We found a high prevalence of periodontitis in groups with increased BMI and the progress of disease severity with the increase in BMI score.Conclusion. The obtained results evidence a high prevalence of chronic generalized periodontitis in overweight and obese patients, which allows us to conclude that overweight and obesity are risk factors for periodontal inflammatory- destructive processes.
The information value of crevicular fluid immunoregulatory mediator quantitative assessment in predicting the nature of the inflammatory periodontal disease course
Authors: Eremin O. V. , Ostrovskaya L. Yu. , Zakharova N. B. , Kathanova L. S. , Kobzeva J. A. , Domenyuk D. A. 
Published: September 2022

Relevance. Clinical and radiological assessment mainly forms the diagnosis of periodontal diseases. The diagnosis now requires speed, sensitivity and specificity since determining the patient's disease stage is fundamental to effective treatment. Crevicular fluid biomarkers can help monitor the current state of the disease, the effectiveness of treatment, and possibly predict the pathological process progression. The combination of various biomarkers will allow maximum objectivity in periodontal tissue condition assessment.Materials and methods. The study examined 72 patients with inflammatory periodontal diseases and 25 periodontally healthy subjects. We performed a conventional clinical-instrumental examination and studied pro- and anti-inflammatory interleukins (IL-1β, IL-6, IL-17, TNF-α, VEGF, IL-8, MCP-1, IL-1RA) in the crevicular fluid. The obtained materials were processed using ROC analysis.Results. Inflammatory periodontal diseases demonstrate an increase in pro-inflammatory cytokines / chemokines (IL-1β, TNF-α, IL-6, IL-17, IL-8, MCP-1) and vascular endothelial growth factor (VEGF) in the crevicular fluid, a decrease in the anti-inflammatory cytokine, IL-1RA. The levels of pro- and anti-inflammatory cytokines, cytokines/ chemokines, VEGF are associated with the periodontal destruction severity caused by inflammation. The accumulation of VEGF, IL-6, and IL-1β in the crevicular fluid predicts the clinical course of gingivitis, VEGF, TNF-α, IL-6, IL-1β – mild and moderate periodontitis.Conclusion. The present study allows us to confirm the diagnostic value of methods for obtaining and quantifying a group of immunoregulatory cytokines in the crevicular fluid as predictors and parameters of the disease progression and the development of osteodestructive changes in the periodontium.
A modern approach to planning and surgical removal of a foreign body from a maxillary sinus: a clinical case
Authors: Lysenko A. V. , Razumova A. Y. , Yaremenko A. I. , Ivanov V. M. , Strelkov S. V. , Grigoriev A. A. 
Published: September 2022

Relevance. If a foreign body is present in a maxillary sinus, it should be surgically removed. Endoscopic and radical surgery are the main methods. Clinician’s subjective feelings determine the surgical access, which can cause complications. Therefore, the search for new methods of planning and visualizing the operation stages remains relevant.Materials and methods. Before the operation, the patient had a cone-beam computed tomography in a marker holder frame. The 3D slicer program allowed the segmentation of the foreign body and surrounding anatomical features. A marker, fixed on the patient's head, allowed transmitting information to the augmented reality glasses during the operation.Results. The surgery was performed under local anesthesia in an outpatient facility. The diameter of the antrotomy hole was 5 mm. No postoperative complications were recorded.Conclusion. The proposed technique provides significant visual control and minimal trauma to the sinus during surgery.