bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of widespread use of EVAR on aneurysm-related mortality in the last decade: A nationwide big data platform-based analysis
Authors: Cho Sungsin , Kim Hyangkyoung , Joh Jin Hyun 
Published: November 2022

Abstract
The introduction of endovascular aneurysm repair (EVAR) for the treatment of abdominal aortic aneurysms (AAA) has led to a dramatic decline in open aneurysm repair (OAR). However, there was no report on the trend of AAA-related mortality after the introduction of EVAR. The aim of this study is to evaluate the trends of annual AAA treatment and AAA-related morality including ruptured AAA (rAAA) and intact AAA (iAAA) last decade. We conducted a serial, cross-sectional study with a time trend of patients who were managed with AAA and underwent aneurysm repair including EVAR and OAR between 2010 and 2020. Data from the Health Insurance Review and Assessment Service (HIRA) were used to evaluate AAA management and repair trends. Data on the annual mortality due to rAAA and iAAA were obtained from Statistics Korea. We evaluated the annual mortality due to AAA and morality rate after the management of rAAA. A linear-by-linear association was performed to determine the changes in the rate of these aneurysm repair techniques. The annual number of patients with iAAA and rAAA increased from 3,686 to 10,630 and 462 to 750 patients, respectively. The number of patients with AAA repair increased from 931 in 2010 to 2,050 in 2020 (OAR; 525 in 2010 to 822 in 2020 and EVAR; 406 in 2010 to 1,228 in 2020). The percentage of EVAR for the treatment of AAA increased from 43.6% in 2010 to 59.9% in 2020. The annual number of AAA-related mortal patients increased from 213 in 2010 to 301 in 2020. However, the annual AAA-related mortality rate decreased from 5.1% in 2010 to 2.6% in 2020. The mortality rate after the management of rAAA did not change during this period (mean mortality rate, 35.7%). During the last decade in Korea, the number of patients with AAA and AAA repair increased. In addition, the AAA-related mortal case increased. But, the AAA-related mortality rate decreased after the increasing trend of EVAR procedure. The nationwide mortality rate after the management of rAAA does not change.
Mir-183 functions as an oncogene via decreasing PTEN in breast cancer cells
Authors: Mohammaddoust Samaneh , Sadeghizadeh Majid 
Published: November 2022

Abstract
Regarding the important role of microRNAs in breast cancer, investigating the molecular mechanisms of miRs and their impacts on breast cancer progression is critical. Thus, the present work aimed to investigate the molecular mechanism of miR-183 in breast cancer. PTEN was validated by dual luciferase assay as a target gene of miR-183. Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured. To determine the impacts of miR-183 on cell viability, the MTT assay was used. Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression. To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. Western blot was utilized to assess the effect of miR-183 on PTEN protein expression. MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. It was revealed that cellular oncogenicity is positively regulated by miR-183 by inhibiting the expression of PTEN. According to the present data, miR-183 may play a vital role in the progression of breast cancer by reducing PTEN expression. It may be also a potential therapeutic target for this disease.
Disseminated nocardiosis attributable to Nocardia farcinica in a context of influenza A virus coinfection and monoclonal gammopathy of undetermined significance: a case report and review of the literature
Authors: Arranz Mario Cárdaba , Rosselló Gabriel Alberto March , Sánchez Gloria Renedo , Peinador Elsa Mucientes , González Francisco Javier Pérez 
Published: November 2022

Abstract
Background
Nocardia species are a rare cause of opportunistic infections that mainly affect immunocompromised patients. Nocardia species have a particular tropism for the brain. We report a fatal case of disseminated nocardiosis associated with influenza A virus coinfection and monoclonal gammopathy of undetermined significance (MGUS) which has never been reported to the best of our knowledge.
Case presentation
A 72-year-old immunosuppressed Caucasian man with influenza A virus infection and a history of MGUS was admitted for probable pneumonia and developed brain space-occupying lesions. Given the timing of disease progression, the response to antibiotics and the brain tropism of the microorganism cultured from an unexpected late-diagnosed primary cutaneous infection, Nocardia farcinica was considered the cause of brain lesions. A delayed diagnosis was given after a circuitous workup. Although combination therapy consisting of trimethoprim/sulfamethoxazole (TMP/SMX), amikacin and amoxicillin-clavulanate resulted in clinical improvement, patient died several weeks after discharge to a long-term care facility (LTCF). Autopsy was not requested.
Conclusions
When immunosuppressed patients develop an obscure infection, nocardiosis must be promptly considered in differential diagnosis. Influenza-associated disseminated nocardiosis impairs survival prognosis. Influenza can pose severe problems for MGUS patients. Effective patient-physician communication, comprehensive history taking, thorough physical examination, the importance of accounting for comorbidities and interprofessional communication and teamwork cannot be overemphasized. Autopsy is an essential tool to improve diagnostic accuracy and would have determined the causes of death.
Negative regulation of CD44st by miR-138-5p affects the invasive ability of breast cancer cells and patient prognosis after breast cancer surgery
Authors: Jian Fang Xin , Bao Peng Xiao , Li Wang Fu , Cui Yan Hai , Hong Hang Guan 
Published: November 2022

Abstract
Objective
To investigate how the negative regulation of CD44st by miR-138-5p affects the invasive ability of breast cancer cell lines and prognosis in postoperative breast cancer patients.
Methods
RT-PCR, qRT-PCR, and western blot assays were used to detect the expression of CD44s, CD44v6, and CD44st at both mRNA and protein levels. The expression of miR-138-5p in breast cancer cell lines was also evaluated. The binding ability of miR-138-5p to CD44st was determined via a dual-luciferase assay. The CD44 protein expression in breast cancer tissues was detected using immunohistochemistry. A Transwell assay was used to detect the invasive ability of tumor cells. The correlation between CD44st and miR-138-5p mRNA expression in breast cancer tissues was evaluated using qRT-PCR, and the relationship between clinicopathological features was statistically analyzed.
Results
CD44s and CD44v6 were highly expressed in MDAMB-231 cell line, while CD44st was highly expressed in MCF-7/Adr and Skbr-3 cells. None of the CD44 isoforms were expressed in MCF-7 cells. The miR-138-5p was highly expressed in MCF-7 cells, but not in MCF-7/Adr, Skbr-3, and MDAMB-231 cells. The dual-luciferase assay suggested that miR-138-5p could bind to wild-type CD44st 3'-UTR, miR-138-5p overexpression significantly inhibited the expression level of CD44 protein in MCF-7/Adr cells, and miR-138-5p + CD44st (3'-UTR)-treated MCF-7/Adr and Skbr-3 cells were significantly less invasive than those in the control group (P < 0.05). RT-PCR results for 80 postoperative breast cancer patients showed that the mRNA expression rate for CD44st was higher in cancer tissues than in paracancerous tissues, and the expression rate of miR-138-5p was higher in paracancerous tissues than in cancerous tissues (P < 0.01). In cancer tissues, CD44st was negatively correlated with miR-138-5p expression, with correlation coefficient r=-0.76 (Pearson’s correlation), coefficient of determination R2 = 0.573, F = 106.89, and P < 0.001. The median overall survival value for patients in the low miR-138-5p expression group was 40.39 months [95% confidence interval (CI): 35.59–45.18 months] and 56.30 months (95% CI: 54.38–58.21 months) for patients in the high-expression group, with a log rank (Mantel-Cox) of 13.120, one degree of freedom, and P < 0.001.
Conclusion
In breast cancer cell lines, miR-138-5p negatively regulated expression of CD44st and affected the invasive ability of tumor cells and patient prognosis after breast cancer surgery.
Comparative Study of Young-old and Old-old People Using Gait Characteristics and Cardiopulmonary Metabolic Energy Consumption
Authors: Kim Yun-Hee , Chung Eunhye , Lee Su-Hyun , Lee Hwang-Jae 
Published: November 2022

Abstract
Background: Walking is an important factor in daily life. Among elderly people, gait function declines with age. In contrast to the many studies revealing gait differences between young adults and elderly people, few studies have divided elderly people into two groups. The purpose of this study was to subdivide an elderly population by age to identify age-related differences in gait characteristics and cardiopulmonary metabolic energy consumption while walking.
Methods: This was a cross-sectional study of 62 elderly participants who were classified into two age groups of 31 participants each as follows: young-old (70.58 ± 2.41 years) and old-old (77.74 ± 2.07 years). A three-dimensional motion capture system (Kestrel Digital RealTime System®; Motion Analysis Corporation, Santa Rosa, CA, USA) and two force plates (TF-4060-B; Tec Gihan, Kyoto, Japan) were used to investigate spatiotemporal parameters, kinematics, and kinetic data of gait. A portable cardiopulmonary metabolic system (K5; Cosmed, Rome, Italy) was used to measure cardiopulmonary energy consumption. Physical functions, activities of daily living, mood state, cognitive function, quality of life, and fall efficacy were evaluated using the Short Physical Performance Battery (SPPB), Four-square Step Test (FSST), Timed Up and Go Test (TUG), 10 Meter Walk Test (10MWT), 6-minute Walk Test (6MWT), Korean Version of the Modified Barthel Index, Geriatric Depression Scale (GDS), Korean Mini-mental State Examination, EuroQol-5 Dimensions (EQ-5D) questionnaire, and the Korean version of the Fall Efficacy Scale.
Results: The old-old group showed significant decreases in SPPB, FSST, TUG, 10MWT, GDS, and EQ-5D scores (p < 0.05). Among the spatiotemporal gait parameters, gait speed, stride length, and step length were significantly lower in the old-old group than in the young-old group (p < 0.05). Hip extension moment at 52% gait cycle (GC), deviation of hip power at 12%–50% GC, knee extension power at 58% GC, and ankle extension angle at 68% GC were significantly reduced in the old-old group compared to in the young-old group (p < 0.05).
Conclusion: This study demonstrates that participants in the old-old group had less functional gaits than those in the young-old group. As the walking pace of old-old people diminishes, the driving strength to move ahead and the pressure on the knee joint also tend to decrease, together with stride length. These differences in gait characteristics according to age among elderly people could improve the understanding of how aging causes variations in gait that increase the risk of falls. Elderly people of different ages may require customized intervention plans, such as gait training methods, to prevent age-related falls.
Effect of Dataset Size and Auxiliary Data in Bayesian Learning of Advanced Manufacturing: A Composite Autoclave Processing Diagnostic Study
Authors: Crawford Bryn , Ramezankhani Milad , Milani Abbas Sadeghzadeh 
Published: November 2022

Abstract
Recent advances in data-driven predictive modelling have enabled the emergence of intelligent manufacturing enterprises. Nonetheless, most of the present frameworks incorporate non-interpretable black-box machine learning models, often requiring large datasets and yet lacking ‘diagnostic’ modelling capabilities. In the context of advanced composites manufacturing, where the presence of numerous decision factors and uncertainties can rapidly yield failures, training cost/data-efficient, transparent and diagnostic-capable predictive models continue to highly valuable to pertinent industries. This can specifically allow decision-makers on the manufacturing floor to identify the causes or state variables of the process that contribute to the product failure (e.g., due to an excessive exotherm or lag temperature during curing), and thereby saving sizable volume of material scraps due to trial and errors. In this work, a Bayesian learning framework with inverse modelling capabilities for an advanced composites autoclave curing process has been developed and assessed for the first time, while assuming different dataset size availabilities. The advantages of using both a naïve Bayesian network and a highly-connected Bayesian belief network (BBN) are compared and discussed. The results revealed that integration of expert knowledge under highly-connected Bayesian models can offer a favorable predictive performance for root cause analyses, along with apparent tractability for in-situ applications, despite the very limited-volume of training data, when accompanied with carefully selected auxiliary data (e.g. via the use of a proxy thermocouple during the processing based on expert domain).
Comparison of clinical and pathological features between early-stage gastric-type and intestinal-type differentiated adenocarcinoma: a retrospective study
Authors: Li Borui , Chen Tingting , Liang Dingbao , Zhang Yin , Ding Xiwei , Lv Ying 
Published: November 2022

Abstract
Background: The clinicopathological features and endoscopic characteristics under magnifying endoscopy with narrow band imaging (ME-NBI)between early-stage gastric-type differentiated adenocarcinoma (GDA) and intestinal-type differentiated adenocarcinoma (IDA) remain controversial.
Methods: Early gastric adenocarcinomas that underwent endoscopic submucosal dissection (ESD) in Nanjing Drum Tower Hospital between August 2017 and August 2021 were included in the present study. GDA cases and IDA cases were selected based on morphology and immunohistochemistry staining of CD10, MUC2, MUC5AC, and MUC6. Clinicopathological data and endoscopic findings in ME-NBI were compared between GDAs and IDAs.
Results: The mucin phenotypes of 657 gastric cancers were gastric (n= 307), intestinal (n=109), mixed (n=181) and unclassified (n=60). No significant difference was observed in terms of gender, age, tumor size, gross type, tumor location, background mucosa, lymphatic invasion, and vascular invasion between patients with GDA and IDA. GDA cases were associated with deeper invasion than IDA cases (p=0.007). In ME-NBI, GDAs were more likely to exhibit an intralobular loop patten, whereas IDAs were more likely to exhibit a fine network pattern. In addition, the proportion of none-curative resection in GDAs was significantly higher than that in IDAs (p=0.007).
Conclusion: The mucin phenotype of differentiated early gastric adenocarcinoma has clinical significance. GDA was associated with less endoscopically resectability than IDA.
Preparation and Properties of Smoke Suppressive Silicone Oil Modified by Dicyandiamide
Authors: Shi Xu , Xu Weibing , Zhou Zhengfa , Ma Haihong , Ren Fengmei 
Published: November 2022

Abstract
In order to improve the smoke suppression of silicone oil, relatively high nitrogen content silicone oil was synthesized by the raction of dicyandiamide (DCD) and epoxy-terminated silicone oil (ETSO) and named DCDSO. The molecular structure of DCDSO was characterized by fourier transform infrared spectrometer (FT-IR) and nuclear magnetic resonance spectrometer (NMR). The flame ignition and cone calorimetry test (CCT) were used to investigate the smoke suppression effect of DCDSO. The results showed that the introduction of DCD significantly improved the smoke suppression performance of DCDSO. When the molar ratio of the epoxy group in ETSO and the primary amino group in DCD was 1:1.3, the flame height and the total smoke production reduced 77% and 53.73%, respectively. Thermogravimetric analysis (TGA), thermogravimetric infrared analysis (TG-IR) and thermal field emission scanning electron microscopy (SEM) were used to explore the mechanism of smoke suppression. The mechanism of smoke suppression is that the introduction of DCD reduces the rearrangement decomposition, promotes the free radical oxidation decomposition and the formation of condensed phase, and thus reduces the generation and release of smoke.
Submarine optical fiber communication provides an unrealized deep-sea observation network
Authors: Ashry Islam , Guo Yujian , Marin Juan , Trichili Abderrahmen , Havlik Michelle , Ng Tien Khee , Duarte Carlos , Ooi Boon S. 
Published: November 2022

Abstract
The ocean is key to human survival, providing natural resources, most of the global oxygen supply, and economic development through mineral, gas, and oil deposits. Although the sea is primarily considered a silent world, it is abundant with the natural sounds of marine life communication and geological processes. Human activities, especially active sonars, shipping traffic, and underwater vehicles, have significantly affected underwater soundscapes and reduced natural sounds. We report on a joint optical fiber-based communication and sensing technology to minimize noise pollution in the ocean while achieving data transmission and ocean environment monitoring and exploration. The proposed multifunctional communication and sensing fiber system enables two-way data transfer while a) monitoring marine life and ship movement near the deployed fiber at the sea bottom and b) sensing temperature. The same fiber is simultaneously used to transfer energy that can be harvested by devices in the internet of underwater things. The proposed approach dramatically reduces the costs and effects of monitoring marine ecosystems while transferring data, monitoring oceans, and charging submerged devices in the internet of underwater things.
ANXA5 promotes glioma progression through the FAk/PI3K/AKT pathway
Authors: Zhang Xinyuan , Zhou Huandi , Han Wei , Zhang Dongdong , Han Xuetao , Hou Liubing , Li Haonan , Sun Guozhu , Xue Xiaoying 
Published: November 2022

Abstract
Objective
Annexin A5 (ANXA5) is a member of the calcium dependent phospholipid binding protein family and participates in the regulation of a variety of physiological and pathological processes. However, whether and how ANXA5 affects the growth and progression of glioma remains unclear.
Methods
We analyzed the expression of ANXA5 in multiple public databases, and verified the expression in 50 glioma clinical samples with Western blotting to study the correlation between ANXA5 and prognosis, survival, and clinical characteristics. In vitro experiments, we up-regulated and down-regulated the expression of ANXA5 in glioma cells, then treated glioma cells with up regulated ANXA5 expression with Y397 FAK specific phosphorylation inhibitor Y15, and verified the effect of ANXA5 on proliferation, migration and invasion of glioma cells through CCK8, clone formation, EDU, mgration and invasion assays, scratching,and Western blotting proved that the expression level of ANXA5 affected the protein level of upstream and downstream factors of FAk/PI3K/AK pathway.
Results
ANXA5 is highly expressed in glioma and is related to clinical features such as grade, age and molecular typing. The higher the expression of ANXA5, the worse the prognosis. The increased expression of ANXA5 can promote the proliferation, migration and invasion of glioma cells, activate FAk/PI3K/AK pathway, and Y15 can reverse the promotion of ANXA5 overexpression; When ANXA5 is knocked down, the result is opposite.
Conclusions
ANXA5 is highly expressed in glioma and is an independent prognostic factor of glioma patients. ANXA5 can promote the proliferation, migration and invasion of glioma cells through FAk/PI3K/AK pathway, and this promotion can be reversed by Y15.
Transjugular intrahepatic collateral-systemic shunt is effective for cavernous transformation of portal vein with variceal bleeding
Authors: Tie Jun , Gou Xiaoyuan , He Chuangye , Li Kai , Yuan Xulong , Jia Wenyuan , Niu Jing , Han Na , Xu Jiao , Zhu Ying , Wang Wenlan 
Published: November 2022

Abstract
Background: The transjugular intrahepatic portal collateral-systemic shunt (transcollateral TIPS) is used to treat portal hypertension-related complications in patients with cavernous transformation of the portal vein (CTPV) and whose main portal vein cannot be recanalized. It is still not clear whether transcollateral TIPS can be as effective as portal vein recanalization–transjugular intrahepatic portosystemic shunt (PVR–TIPS). This study aimed to evaluate the efficacy and safety of transcollateral TIPS in the treatment of refractory variceal bleeding with CTPV.
Methods: Patients with refractory variceal bleeding caused by CTPV were selected from the database of consecutive patients treated with TIPS in Xijing Hospital from January 2015 to May 2021. They were divided into the transcollateral TIPS group and the PVR–TIPS group. The rebleeding rate, overall survival, shunt dysfunction, overt hepatic encephalopathy (OHE) and operation-related complications were analyzed.
Results: A total of 192 patients were enrolled, including 21 patients with transcollateral TIPS and 171 patients with PVR-TIPS. Compared with the patients with PVR-TIPS, the patients with transcollateral TIPS had more noncirrhosis (52.4% vs. 19.9%, p = 0.002), fewer splenectomies (14.3% vs. 40.9%, p = 0.018), and more extensive thrombosis (38.1% vs. 15.2%, p = 0.026). There were no differences in rebleeding, survival, shunt dysfunction, or operation-related complications between the transcollateral TIPS and PVR-TIPS groups. However, OHE was significantly lower in the transcollateral TIPS group (9.5% vs. 35.1%, p = 0.018).
Conclusion: Transcollateral TIPS is an effective treatment for CTPV with refractory variceal bleeding.
The PBL teaching method in Neurology Education in the Traditional Chinese Medicine undergraduate students: An Observational Study
Authors: Kim Yun Jin 
Published: November 2022

Abstract
Background and Objective: To study the effect of the Problem Based Learning (PBL) method in Neurology education for Traditional Chinese Medicine (TCM) undergraduate students.
Methods: In this observational study was conducted 2020/02 and 2020/04 intake the year three TCM undergraduate students of the School of Traditional Chinese Medicine, Xiamen University Malaysia. A total of 86 were enrolled in this study. They were randomly divided into conventional learning groups and PBL groups. Students who missed more than one session of the course and those who did not complete the questionnaires in the evaluation periods were excluded from the study (n=0). An independent sample t-test was used to compare the results between the two groups. A p-value <0.05 was considered significant.
Results: The PBL group was significantly effective for the students’ theoretical and clinical practical examination scores, the satisfaction of teaching level, students’ perspectives, and self-learning skills, as well as significantly higher Dundee Ready Educational Environment Measure (DREEM) scores than students who participated with the conventional group (p<0.05).
Conclusion: The PBL teaching method in Neurology education for TCM undergraduate students can involve an interesting learning method, significantly improve their learning performance, and the ability to analyze the problem-solving skills in the neurology disease and its management knowledge.