bluuebunny/crossref_metadata_2025_split huggingface dataset:
Never in mitosis gene A related kinase-6 deficiency deteriorates diabetic cardiomyopathy via regulating Heat shock protein 72
Authors: Shao Shuangyin , Xiao Lili , Jia Meng , Zhang Chuyang , Zhao Guojun , Yao Rui , Wang Xiaofang , Gao Lu 
Published: July 2022

Abstract
NIMA (never in mitosis, gene A)-related kinase-6 (NEK6) a cell cycle regulatory gene was found to regulate cardiac hypertrophy. However, it’s role in diabetic cardiomyopathy (DCM) have not been fully elucidated. This research was designed to illustrate the effect of NEK6 involved in DCM. Here we used a streptozotocin (STZ) induced mice diabetic cardiomyopathy model and NEK6 knockout mice to explore the role and mechanism of NEK6 on DCM. NEK6 knockout mice and wild type littermates were subjected to STZ injection (50mg/kg/d for five constitute days) to induce DCM model. As a result, 4 months after final STZ injection, DCM mice revealed cardiac hypertrophy, fibrosis and systolic and diastolic dysfunction. NEK6 deficiency caused deteriorated cardiac hypertrophy, fibrosis as well as cardiac dysfunction. Moreover, we observed serve inflammation and oxidative stress in NEK6 deficiency mice heart under DCM pathology. We used an adenovirus to overexpress NEK6 in neonatal rat cardiomyocytes, and found that NEK6 ameliorated high glucose induced inflammation, and oxidative stress. Mechanismly, we found that NEK6 increased phosphorylation of heat shock protein 72 (HSP72) and enhanced the protein level of PGC-1α, NRF2. Co-IP assay experiment confirmed that NEK6 interacted with HSP72. When we silence HSP72 the anti-inflammation and anti-oxidative stress effects of NEK6 were blurred. In summary, NEK6 could protect diabetic induced cardiomyopathy via interacting with HSP72 and promoting the HSP72/PGC-1α/NRF2 signaling.
Design and Modelling of Defective Single and Double Layered Graphene : Nano Resonators
Authors: Makwana Manisha , Patel Ajay M 
Published: July 2022

Abstract
Nano sensors and actuators are frequently made of graphene. Any defect in the graphene's manufacturing has an impact on its sensing performance and on its dynamic behaviour. Using a molecular structure mechanics technique, the influence of pinhole defects and atomic defects on the performance parameters of single layer graphene sheets (SLGS) and double layer graphene sheets (DLGS) with various boundary conditions and lengths is explored. In contrast to the perfect nanostructure of a graphene sheet, defects are described as holes formed by atomic vacancies. As the number of defects increases, the simulation results show that the presence of defects has the greatest impact on the resonance frequency of SLG and DLG. The interlayer separation in the form of Van der Waals interaction is modelled using characteristic spring element. The upper and lower sheets of DLGS are described as elastic beams connected by a spring element. For the purposes of analysis, two distinct types of boundary conditions (BD) are identified: cantilever and bridged. The influence of pinhole defect (PD) and atomic vacancy defect (AVD) on armchair, zigzag, and chiral SLGS and DLGS was investigated in this article using FEM based atomic Molecular Structure. The influence of both types of defects is largest when it is adjacent to the fixed support for all three different types of graphene sheets, i.e., Armchair, Zigzag, and Chiral. The model has been tested against previous research. The focus of this research is to develop a mechanism for determining how defects affect graphene frequency band.
Combined transcriptome and metabolite profiling analyses provide insights into the chronic toxicity of carbaryl and acetamiprid to Apis mellifera larvae
Authors: Gao Jing , Yang Yang , Ma Shilong , Liu Feng , Wang Qiang , Wang Xing , Wu Yanyan , Zhang Li , Liu Yongjun , Diao Qingyun , Dai Pingli 
Published: July 2022

Abstract
Despite many studies have revealed that developing honey bee (Apis mellifera) larvae are posting a high risk on exposure to insecticides, but the toxicology information on bee larvae remain limited. The present study demonstrated the first assessment of the effects of no observed adverse effect concentration (NOAEC) of carbaryl (CR) and acetamiprid (ACE) on transcriptome and metabolome in honeybee larvae rearing in vitro. Chronic exposure to carbaryl caused a transcriptional response to oxidative stress. In addition, a series of metabolic homeostasis disrupted by carbaryl stress, such amino acid metabolism, purine and pyrimidine metabolism and flavone and flavonol biosynthesis. The activities of enzymic biomarkers including GST, P450, CAT, AChE and SOD were not influenced by ACE stress, while the CR exposure slightly decreased the activity of CAT and SOD. Our results clearly show that ACE and CR varied in their influence on modulation of transcriptome and metabolome, potentially associated with their different toxicity against bee larvae.
Radiomics analysis and metastatic patients: can we really continue to sidestep intra-patient tumor heterogeneity ?
Authors: Henry Théophraste , Sun Roger , Lerousseau Marvin , Estienne Théo , Robert Charlotte , Besse Benjamin , Robert Caroline , Paragios Nikos , Deutsch Eric 
Published: July 2022

Abstract
After promising results for localized cancer disease, radiomics analysis has been applied to the metastatic setting, sidestepping the potential intertumoral intrapatient heterogeneity captured by radiomics features. Our aim was to evaluate if radiomics features can capture intertumoral intrapatient heterogeneity, and the impact of tumor subsampling on the computed heterogeneity. To this end, We delineated all visible tumors from single acquisition pre-treatment computed tomography of patients with metastatic lung cancer or melanoma, and extracted tumor radiomics features. All robust radiomics indexes exhibited at least 20% of variation with significant heterogeneity both in heavily and oligo metastasized patients, and also at the organ level. None of the segmentation subsampling strategies were able to recover the true tumoral heterogeneity obtained by exhaustive tumor sampling. Further, we discussed potential limitations of predictive algorithms when this kind of heterogeneity is not taken into account.
Modeling the Relationship between the Land Use/Land Cover Change and Land Surface Temperature in Ibadan, Oyo State, Nigeria
Authors: Olofin Emmanuel Oluwafemi , Adebayo Williams Oluwole , Akintola Olayiwola Akin 
Published: July 2022

Abstract
This study was designed to model the spatio-temporal pattern of Land Use/Land Cover (LU/LC) indices and its relationship with Land Surface Temperature (LST) in Ibadan Metropolis from 1988 to 2018. Four Landsat images of Ibadan metropolis for 1988, 1999, 2013 and 2018 were used in this study. Land surface temperature values of vegetation, open surfaces and built-up Land Use/Land Cover (LU/LC) analysis was carried out using ERDAS IMAGINE 9.2 in order to ascertain the areas covered by each land use type. The results of the study revealed that as the size of Built-up land use type increases and the size of the vegetation land use type decreases, the value of land surface temperature increases in relation to the increase in the size of built-up land use type and the decrease in the size of vegetation land use type from 1988 to 2018 in Ibadan Metropolis. This study can be useful in designing Urban Green Belts (UGB) and parks within the metropolis in order to mitigate problem of urban thermal discomfort in Ibadan, the state capital of Oyo State, Nigeria.
Cross sectional analysis of dietary quality among pregnant women in central South Africa: The NuEMI study
Authors: Berg Violet Louise van den , Jordaan Elizabeth Margaretha , Robb Liska , Ngounda Jennifer , Nel Mariette , Walsh Corinna May 
Published: July 2022

Abstract
Background: During pregnancy, poor dietary quality is linked to maternal complications and poor birth outcomes, but no dietary quality index for pregnancy has been developed using African data. This study aimed to determine the diet quality of South African pregnant women by applying the Diet Quality Index Adapted for Pregnant Women (IQDAG), developed in Brazil, to data collected in the NuEMI (Nutritional status of Expectant Mothers and their newborn Infants) study.Methods: The NuEMI study applied a descriptive study design to collect data from 681 pregnant women visiting an antenatal clinic at a regional hospital in Mangaung, central South Africa. The IQDAG was applied to dietary data collected with a quantitative food frequency questionnaire during structured interviews. Energy and nutrient intakes were quantified with the South African Medical Research Council Food Composition Database. The Women’s Dietary Diversity Score (WDDS) was applied to 24h-recall data obtained during the interviews. Associations of IQDAG scores, nutrient intakes, sociodemographic variables, WDDS and birth anthropometry were explored by contingency tables.Results: Participants had a median age of 31.9 years (IQR: 26.8‒36.7) and a median pregnancy stage of 32.0 weeks (IQR: 26.0‒36.0). The median total dietary quality score (61/100; IQR:53‒68) indicated that most of these women had a suboptimal diet. Only 2.5% had a good dietary quality, reaching at least 80% of the maximum IQDAG score that represents the minimum pregnancy recommendations. The median intakes for none of the nine components of the IQDAQ met the recommendations. Fruit, vegetables, legume, dietary calcium and omega-3 intakes were particularly low, while the intake of ultra-processed foods was above optimal. Most participants consumed only half of the recommended amounts of iron and folate from their diets. IQDAG scores were significantly (p<0.0001) associated with the dietary intakes of most nutrients, even after correction for energy intakes, and with dietary diversity (p<0.0001) and length-for-age z scores at birth (p=0.01).Conclusions: Pregnant women In a South African public health setting had poor to suboptimum dietary quality. Improving the dietary quality of pregnant women in low to middle-income countries would reduce the high financial and human cost of poor birth outcomes and maternal complications. The results also emphasise the importance of prenatal micronutrient supplementation.
A first approach to the estimation of seismic damage in Guatemala City: a seismic exposure model
Authors: Migoya Luis Diego Dávila , Ballarín José Manuel Cabrero , Beltetón Omar Flores 
Published: July 2022

Abstract
This study presents the analysis of the methodology of a seismic exposure model in Guatemala City. In the last years, progress in the investigation of regional seismic hazards has revealed the need to study the vulnerability of the buildings in Central America. In addition, in recent years there have been earthquakes of moderate magnitude in Guatemala causing damage in some areas of the country. An accurate exposure model and updated threat scenarios are essential for a detailed vulnerability study. This exposure study reveals the presence of structural typologies that are very vulnerable to earthquakes: adobe and simple masonry. Similarly, the study reveals the majority presence of reinforced masonry and the growing construction of reinforced concrete buildings. The characteristic of this model is the use of historical photographs of Guatemala City to determine the relationship between constructive typologies. The lack of exposure studies in the country has prevented the creation of policies to reduce vulnerability to a catastrophe like the earthquake in 1976. The analysis and methodology presented in this study is the best instrument for mitigation and prevention of actions to face a seismic catastrophe. Based on the results obtained, some matrices are proposed according to the age and material of the buildings. In this case, the application of the study contributes to defining the different measures for the reduction of seismic risk in Guatemala City.
Bone Loss is Ameliorated by Fecal Microbiota Transplantation through SCFA/GPR41/ IGF1 Pathway in Sickle Cell Disease Mice
Authors: Xiao Liping , Zhou Yanjiao , Bokoliya Suresh , Lin Qingqi , Hurley Marja 
Published: July 2022

Abstract
Bone loss is common in sickle cell disease (SCD), but the molecular mechanisms is unclear. Serum insulin-like growth factor 1 (IGF1) was low in SCD subjects and SCD mice. To determine if decreased IGF1 associated with low bone mass in SCD is due to reduced SCFA production by gut microbiota, we performed reciprocal fecal microbiota transplantation (FMT) between healthy control (Ctrl) and SCD mice. uCT and histomorphometry analysis of femur showed decreased bone volume/total volume (BV/TV), trabecular number (Tb.N), osteoblast surface/bone surface (Ob.S/BS), mineralizing surface/ bone surface (MS/BS), inter-label thickness (Ir.L.Th) in SCD mice were significantly improved after receiving Ctrl feces. Bone formation genes Alp, Col1, Runx2, and Dmp1 from SCD mice were decreased that were rescued after FMT from Ctrl feces. Transplantation of Ctrl feces increased the butyrate, valerate, and propionate levels in cecal content of SCD mice. Decreased G-coupled protein receptors 41 and 43 (GPR41 and GPR43) mRNA in tibia and lower IGF1 in bone and serum of SCD mice were partially restored after FMT from Ctrl feces. These data indicate that the healthy gut microbiota of Ctrl mice is protective for SCD bone loss through regulating IGF1 in return for impaired bacterial metabolites SCFAs.
Navigating Nutrition as a Childhood Cancer Survivor: Understanding Patient and Family Needs for Nutrition Support
Authors: Clarke Emma , Pugh Gemma , Heuvel Eveline van den , Kavanagh Erin , Cheung Pamela , Wood Andrew , Winstanley Mark , Braakhuis Andrea , Lovell Amy 
Published: July 2022

Abstract
Purpose: Nutrition challenges are common during childhood cancer treatment and can persist into survivorship, increasing the risk of non-communicable chronic diseases. Evidence based practice and implementation of nutrition support for childhood cancer survivors (CCS) has been poorly investigated and may influence the future health of survivors. This study aimed to explore the nutrition support needs of CCS and the barriers and facilitators to delivering follow-up services in New Zealand (NZ).Methods: Qualitative thematic analysis of semi-structured interviews were performed at a specialist paediatric oncology centre in NZ with CCS and their family (N = 20 individuals) and health professionals (N = 9). Results: Three key themes emerged from the analysis: 1) the current survivorship care pathway does not provide adequate nutrition support, 2) weight and dietary changes are common challenges, and 3) requirements for nutrition support in survivorship are varied. Common nutrition-related concerns included fussy eating, poor diet quality, difficulties tube weaning, and challenges with weight gain. Participants expressed a desire for education on healthy eating alongside information about cancer-related nutrition issues, such as learned food aversions. A preference for clear referral pathways and multifaceted interventions tailored to individual patient needs were identified by CCS and health professionals. Conclusion: The trifecta of treatment side effects, negative feeding practices, and poor messaging from health professionals creates a challenging environment to optimise nutrition. A stepped care model matching the intervention intensity with the CCS is required. Education for healthcare professionals will improve the delivery of timely nutrition support and monitoring practices.
Analysis of a Closed-Loop Digital Twin Using Discrete Event Simulation
Authors: Eyring Andrew , Hoyt Nathan , Tenny Joe , Domike Reuben , Hovanski Yuri 
Published: July 2022

Abstract
Given recent advancements in technology and recognizing the evolution of smart manufacturing, the implementation of digital twins for factories and processes is becoming more common and more useful. Additionally, expansion in connectivity, growth in data storage, and the implementation of the Industrial Internet of Things (IIoT) allow for greater opportunities not only with digital twins but closed loop analytics. Discrete Event Simulation (DES) has been used to create digital twins and in some instances fitted with live connections to closely monitor factory operations. However, the benefits of a connected digital twin are not easily quantified. Therefore, a test bed demonstration factory was used, which implements smart technologies, to evaluate the effectiveness of a closed-loop digital twin in identifying and reacting to trends in production. This involves a digital twin of a factory process using DES. Although traditional DES is typically modeled using historical data, a DES system was developed which made use of live data with embedded machine learning to improve predictions. This model had live data updated directly to the DES model without user interaction, creating an adaptive and dynamic model. It was found that this DES with machine learning capabilities typically provided more accurate predictions of future performance and unforeseen near future problems when compared to the predictions of a traditional DES using only historic data, resulting in smarter decisions and implementation of more timely solutions.
Application of constrained implants hip arthroplasty in patients with hip neuromuscular disease
Authors: Zhang Yanfang , Liu Zeming , Liu Sikai , Li Mengnan , Liu Bo , Han Yongtai , Wu Tao 
Published: July 2022

Abstract
Background
It is well known that neuromuscular diseases increase the risk of complications after hip replacement. The purpose of this study was to investigate the clinical manifestation and survival rate of total hip arthroplasty (THA) with constrained implants in inpatients with neuromuscular diseases.
Methods
A retrospective study evaluated the results of 17 THA in 17 patients with hip neuromuscular disease from June 2018 to April 2021. The average age at the time of operation was 66.71 years (33–86 years). The average follow-up time was 28.5 months (6–48 months). All surgery were operated through posterolateral approach. These are cementless implants.
Results
The incisions of all cases healed in one stage, without infection, injury of important nerves, blood vessels and other tissues, loosening of joint prosthesis and deep venous thrombosis of lower limbs. One of the patients developed a new cerebral infarction at postoperative, which affected the contralateral limb movement and seriously affect the postoperative efficacy observation. The otherswere followed up for 6 months to 48 months, with an average of (28.63 ± 13.10) months. All patients underwent THA with constrained implants. The hip joint function was evaluated according to Harris Hip score standard, excellent in 2 cases, good in 12 cases and poor in 2 cases.
Conclusion
THA combined with constrained implants has been shown to be effective in reducing the risk of dislocation and enhancing stability for patients with neuromuscular disease.
Modification of immune response by bacteriophage alters methicillin resistant Staphylococcus aureus infection
Authors: Suda Tomoya , Hanawa Tomoko , Tanaka Mayuko , Tanji Yasunori , Miyanaga Kazuhiko , Hasegawa-Ishii Sanae , Shirato Ken , Kizaki Takako , Matsuda Takeaki 
Published: July 2022

Abstract
There is an urgency to develop phage therapies for multi-drug resistant bacterial infections. However, although bacteria can be shown to be susceptible to phage therapy, it is not sufficient in some cases. PhiMR003 is a methicillin-resistant Staphylococcus aureus (MRSA) phage previously isolated from sewage influent and demonstrates a highly lytic activity and a broad host range to MRSA clinical isolates in vitro. To investigate the potential of phiMR003 for the treatment of MRSA infection, the effects of phiMR003 on immune responses in vivo were analyzed using phiMR003 susceptible MRSA strains in a mouse wound infection model. Additionally, we assessed whether phiMR003 affects the immune response to infection caused by a non-susceptible MRSA strain. Interestingly, cases with both susceptible and non-susceptible MRSA strains infected with phiMR003 demonstrated decreased bacterial load, repressed inflammation and acceleration of wound closure. Moreover, the infiltration of inflammatory cells in infected tissue was altered by phiMR003. All these effects required the phage virion suggesting a phage-specific recognition and signaling response. Induced transcripts of proinflammatory cytokines by lipopolysaccharide (LPS) were repressed in mouse peritoneal macrophages. These results show that immune modulation occurring as a response to the phage itself improves the clinical outcomes of phage therapy.
Comparative assessment of some economic factors influencing environmental pollution in Iwo and Ibadan metropolis, southwestern Nigeria
Authors: Ogunbode Timothy O. , Jazat Joseph P. , Akande John A. 
Published: July 2022

Abstract
Environmental quality is a sustainability index and is also influenced by economic realities. In order to understand the practical values of related components, on area basis, a comparative study of economic factors which influence pollution in Iwo and Ibadan metropolis was carried out. The data for the study was generated through the administration of a total 1,400 structured questionnaires in both locations out of which 215 and 658 were retrieved from Iwo and Ibadan metropolis respectively. The survey showed that respondents of male gender, married status, tertiary education, Christianity sect, household size of 2 to 5 were 52%, 67%,83%, 67.1% and 55% respectively for Iwo, while it was 67%, 61%, 86%, 68%, 71.2% and 57% in the same order for Ibadan. Six economic factors were identified namely (1) Income level (2) accommodation and living standard (3) waste/noise management regimen (4) energy utilization (5) orthodox versus green economy status and (6) waste sorting. Kaiser-Meyer-Olkin sampling adequacy and Bartlett’s test of sphericity showed that the data was factorable at p < 0.005. The results of the analyses showed that three different economic variables each gave significant explanations for the pollution in Iwo and Ibadan. The significant variables extracted for Iwo explained 59.3% of the factors which were waste/noise management strategies (22.5%), accommodation and living standard (18.7%) and inclination towards green economy (18.3%). The three factors extracted for Ibadan explained 60.2% effect of all economic variables on pollution. These were accommodation and living standard (24.4%), inclination towards green economy (18.8%) and waste/noise management strategies (17.0%). Though two of the variables namely accommodation and living standard, and inclination towards green economy were common to both study areas, their respective importance and ordering varied. While waste and noise management were most significant in Iwo, the same variable had the least effect in Ibadan. Similarly, while inclination towards green economy was lowest in Iwo, it was the most significant in Ibadan. It is therefore concluded that the three economic factors influencing pollution in Iwo and Ibadan metropolis, though similar cannot be given a generalized weighting. In other words, targeted efforts towards resolving pollution-related matters from economic viewpoint should be specific for each administrative or local government area.