bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic Significance of Early Molecular Response in Patients Diagnosed with Chronic Myeloid Leukemia in Chronic Phase Treated with Nilotinib as a First-Line Therapy
Authors: Shazly Mohammad Abdallah , Azzazi Mohammed Osman , Samra Mohamed , Elsaed Ahmed Youssri 
Published: October 2022

Abstract
Background: Chronic myeloid leukemia (CML) is one of malignant hematologic disorders arises from hematopoietic stem cells. BCR-ABL transcript levels on the international scale at 3 and 6 months are defined as indicators of the early efficacy of first line TKI treatment.
Aim To investigate the impact of early molecular response (EMR; BCR-ABL ≤ 10% on the International scale at 3 or 6 months) on outcome of the newly diagnosed CML in chronic phase treated with Nilotinib.
Patients and Methods: The study was enrolled from 2018 to 2020 at Nasser Institute for Research and Treatment.This is a prospective cohort study done on 94 newly diagnosed CML cases in Chronic Phase.
Results: A statistically significant difference was detected between patients not achieved EMR with peripheral blasts ≥5%, when compared to others achieved EMR with peripheral blasts <5% (P<0.001). 75% of patients not achieved EMR were ≥55 years age at diagnosis; and 90% of patients achieved EMR were <55 years of age at diagnosis with (P<0.001). 25% of cases not achieved EMR were compliant, while other cases achieved EMR were compliant with (P<0.001).Overall survival remained higher in patients who achieved EMR (N=90) compared to patients who did not achieve EMR (N=4) (P=0.0001).
Conclusion: EMR is an important prognostic significance for CML patients received treatment with Nilotinib. Patients who achieved EMR had significantly better outcome. Achieving MR3.0 should be the aim in patents with CML-CP who have a 3-month BCR-ABL ≤ 10% and 6-month BCR-ABL ≤ 10%.
Experimental results of permittivity of lunar simulated materials in the UHF-SHF band for radar explorations
Authors: Kobayashi Makito , Miyamoto Hideaki , Bernadett Pál Diána , Niihara Takafumi , Takemura Tomohiro 
Published: October 2022

Abstract
The mapping of available water-ice is a crucial step in the lunar exploration missions. Ground penetrating radars have the potential to map the subsurface structure and the existence of water-ice in terms of the electromagnetic properties, specifically, the permittivity. Slight differences in permittivity can be significantly important when applied in a dry environment, such as on the Moon and Mars. The capability of detecting a small fraction of putative water ice depends on the permittivity changes in terms of its dependent parameters, such as the frequency, the temperature, the porosity, and the chemical composition. Our work aims at mitigating false detection or overlooking of water-ice by considering their conditions that previous researches did not cover. We measured the permittivity of different lunar regolith relevant samples with a fixed 40 \% porosity in the ultra high frequency - super high frequency band. We used the coaxial probe method to measure anorthosite, basalt, dunite and ilmenite at 20 °C, -20°C and -60°C. We find that, at – 60 °C, the permittivity decreases about 7 – 20 \% compared with the values at 20 °C, and that permittivity changes caused by ilmenite content could lead to false detection of lunar water-ice mostly. Our results also suggest that the water-ice content could be estimated from radar measurements at different temperatures.
Global transcriptome profiling reveals differential regulatory, metabolic and hormonal networks during somatic embryogenesis in Coffea arabica
Authors: Awada Rayan , Lepelley Maud , Breton David , Charpagne Aline , Campa Claudine , Berry Victoria , Georget Frédéric , Breitler Jean-Christophe , Léran Sophie , Djerrab Doâa , Martinez-Seidel Federico , Descombes Patrick , Crouzillat Dominique , Bertrand Benoît , Etienne Hervé 
Published: October 2022

Abstract
Background
Somatic embryogenesis (SE) is one of the most promising processes for large-scale dissemination of elite varieties. However, for many plant species, optimizing SE protocols still relies on a trial and error approach. We report the first global scale transcriptome profiling performed at all developmental stages of SE in coffee to unravel the mechanisms that regulate cell fate and totipotency.
Results
RNA-seq of 48 samples (12 developmental stages x 4 biological replicates) generated 90 million high quality reads per sample, approximately 74% of which were uniquely mapped to the Arabica genome. First, the statistical analysis of transcript data clearly grouped SE developmental stages into seven important phases (Leaf, Dedifferentiation, Primary callus, Embryogenic callus, Embryogenic cell clusters, Redifferentiation and Embryo) enabling the identification of six key developmental phase switches, which are strategic for the overall biological efficiency of embryo regeneration. Differential gene expression and functional analysis showed that genes encoding transcription factors, stress-related genes, metabolism-related genes and hormone signaling-related genes were significantly enriched. Second, the standard environmental drivers used to control SE, i.e. light, growth regulators and cell density, were clearly perceived at the molecular level at different developmental stages. Third, expression profiles of auxin-related genes, transcription factor-related genes and secondary metabolism-related genes were analyzed during SE. Gene co-expression networks were also inferred. Auxin-related genes were upregulated during dedifferentiation and redifferentiation while transcription factor-related genes were switched on from the embryogenic callus and onward. Secondary metabolism-related genes were switched off during dedifferentiation and switched back on at the onset of redifferentiation. Secondary metabolites and endogenous IAA content were tightly linked with their respective gene expression. Lastly, comparing Arabica embryogenic and non-embryogenic cell transcriptomes enabled the identification of biological processes involved in the acquisition of embryogenic capacity.
Conclusions
The present analysis showed that transcript fingerprints are discriminating signatures of cell fate and are under the direct influence of environmental drivers. A total of 23 molecular candidates were successfully identified overall the 12 developmental stages and should now be tested in many plant species to optimize SE protocols in a rational way.
The gut microbiota affects amyloid deposition in Alzheimer’s disease models by suppressing immune-mediated Aβ clearance
Authors: Wasén Caroline , Vincentini Julia , Gauthier Christian , Lopes Juliana , Butovsky Oleg , Cox Laura , Weiner Howard 
Published: October 2022

Abstract

The gut microbiota and innate immune system play critical roles in Alzheimer’s disease (AD). Bacteroides is elevated in AD patients and correlates with cerebrospinal fluid levels of Aβ and tau. We found that increased Aβ plaques in Bacteroides fragilis-treated APP/PS1-21 mice were associated with altered cortical expression Aβ processing genes. B. fragilis suppressed peripheral CD4+ T cell production of GM-CSF and IL-4 and transcriptional changes in microglia related to GM-CSF and IL-4 signaling, phagocytosis, and protein degradation. Furthermore, B. fragilis impaired the microglial uptake of intracranially injected Aβ42, whereas Erysipelotrichaceae strains increased uptake. Depleting murine Bacteroidetes with metronidazole decreased amyloid load in aged 5xFAD mice, increased CD4+ T cell GM-CSF production, and activated microglial pathways related to cytokine signaling, phagocytosis and lysosomal degradation. These data suggest that the gut microbiome may contribute to AD pathogenesis by suppressing peripheral cytokines and microglia phagocytic function, leading to impaired immune-mediated Aβ clearance.
Development and Validation of the Children’s STEM Habits of Mind Questionnaire
Authors: Yang Weipeng , Du Yingqiao , Wu Rongxiu , Xiang Sixuan 
Published: October 2022

Abstract
Implementing STEM (science, technology, engineering, and mathematics) education in early childhood education has received increasing attention in recent years. STEM process skills developed during the early years have lifelong positive effects on young children. In this study, we developed the Children’s STEM Habits of Mind Questionnaire (CSHMQ) and validated it in a sample of 231 Chinese preschoolers (Mage = 4.77 years, SD = 1.19). Items in the questionnaire were generated from literature and selected based on the factor analysis to ensure the scope, content, and construct validity of the scale. Results revealed Science Process Skills, Encoding and Decoding Skills, and Engineering and Numeracy Skills as the three dimensions of the questionnaire with high internal reliability. The total score of the questionnaire and the three sub-factor scores were all positively correlated with children’s age, which demonstrates its preliminary validity. CSHMQ provides a professional tool for early childhood researchers and practitioners to assess children’s STEM process skills, thus providing evidence to plan and adjust STEM pedagogical practices to better promote children’s development.
Influence Mechanism of Gluing Process on the Strength of Clinch-Adhesive Hybrid Joining Process of Steel/Aluminum Plates
Authors: Wang Menghan , Du Menglong , Chen Yifeng , Han Yan , Li Lei 
Published: October 2022

Abstract
To solve the problems of low strength and unsatisfactory connection quality of single joining technology, a method of clinch-adhesive hybrid joining of steel/aluminum sheets with partially glued and inclined wall die was proposed. Al5052 aluminum alloy and Q235 steel were used as research objects, finite element simulation combined with actual experiments was adopted, the effect of the gluing process on the strength of clinch-adhesive hybrid joints on the inclined wall die was explored, and the failure forms and failure mechanisms of the joints in shear and peel experiments were studied. Results showed that the effect of partially glued is better than that of complete gluing. And the partial gluing process is more effective than the complete gluing process. The interlock of partially glued hybrid joints was increased by 144\% compared to fully glued joints, but the neck thickness value was reduced by only 10.6\%. The shear strength and peel strength of partially glued hybrid joints are higher than fully glued hybrid joints and clinch joints. The failure mode of the hybrid joints under shear and peel loads is mixed failure composed of interface failure and upper sheet separation failure.
Impact of taste/smell disturbances on dietary intake and cachexia-related quality of life in patients with advanced cancer
Authors: Otani Hiroyuki , Amano Koji , Morita Tatsuya , Miura Tomofumi , Mori Naoharu , Tatara Ryohei , Kessoku Takaomi , Matsuda Yoshinobu , Tagami Keita , Mori Masanori , Taniyama Tomohiko , Nakajima Nobuhisa , Nakanishi Erika , Kako Jun , Shirado Akemi Naito , Yokomichi Naosuke , Miyashita Mitsunori 
Published: October 2022

Abstract
Purpose
All five senses (i.e., sight, smell, hearing, taste, and touch) are used to enjoy meals; however, impairments in sensory perception, which are common among cancer patients, seriously impact health and cause eating-related distress.
Methods
Using a self-report questionnaire, we surveyed patients with advanced cancer undergoing treatment at 11 palliative care centers. Multivariate analyses were conducted to explore the impact of taste and smell disturbances on dietary intake and cachexia-related quality of life (QOL). Dietary intake was assessed using the ingesta-Verbal/Visual Analog Scale, while taste and smell disturbances were assessed using an 11-point Numeric Rating Scale (NRS). Cachexia-related QOL was assessed using the 12-item Functional Assessment of Anorexia/Cachexia Therapy (FAACT) Anorexia/Cachexia Subscale.
Results
Overall, 378 patients provided consent to participate. After excluding patients with missing data, data were analyzed for 343 patients. Among them, 35.6% (n = 122; 95% CI 0.28–0.38) and 20.9% (n = 72; 95% CI 0.17–0.25) experienced disturbances in taste (NRS ≥ 1) and smell (NRS ≥ 1), respectively. Multivariate analyses revealed that, independent of performance status and cancer cachexia, taste and smell disturbances were significantly associated with worse FAACT Anorexia/Cachexia Subscale scores and dietary intake.
Conclusion
More severe taste and smell disturbances were associated with poorer dietary intake and cachexia-related QOL. Diagnosing and treating such disturbances may improve dietary intake and cachexia-related QOL, regardless of performance status and cachexia. Additional research is required to establish effective treatment strategies for disturbances in taste and smell.
Magnetic Field and Nuclear Spin Influence on the DNA Synthesis Rate
Authors: Stovbun Sergey , Zlenko Dmitry , Bukhvostov Alexander , Vedenkin Alexander , Skoblin Alexey , Kuznetsov Dmitry , Buchachenko Anatoly 
Published: October 2022

Abstract
Chemical reactions can be isotope-sensitive and alter the isotope composition in the products concerning that in the initial compounds. Besides that, the rate and, probably, the pathway of certain reactions can depend on the nuclear spin of the atoms participating in the reaction. The sensitivity to the atoms' spin implies the sensitivity of the "spin-sensitive" reactions to the magnetic field as it is, regardless if this field is an external one or originated from the spin of some atoms. Here we demonstrate the effect of the external magnetic field on the enzymatic DNA synthesis together with the effect of the magnetic magnesium ions. The rate of DNA synthesis monotonously decreased with the external magnetic field intensity in presence of the non-magnetic magnesium ions. On the contrary, in the presence of the spin-bearing ions, the dependence of the rate on the magnetic field intensity was nonmonotonous and possess a distinct minimum at 800-1000 G. We also suggest a scheme explaining the observed effects.
Assessment of Pubertal Growth Spurt in Egyptian Adolescent Girls Using Middle Phalanx of the Middle Finger (MP3) and its Correlation with BoneRadiodensity. (Cross sectional study)
Authors: Alrubaie Hasan Abdullah , Elboghdady Dalia Mohamed , aziz Sahar Taher Abdel , Foda Manal Yehia 
Published: October 2022

Abstract
Objectives: to assess the pubertal growth spurt in Egyptian adolescent girls, using middle phalanx of the middle finger (MP3) skeletal maturity indicator. Additionally, to assess the (MP3) radiodensity and to correlate the bone radiodensity with pubertal stages.
Materials and methods: This study was carried out at Alexandria governorate, Egypt, between the period of 9/2016 till 9/2017. A total sample of 1257 girls were included, with an age range of (8 -14 years). Middle phalanx of the middle finger (MP3) was digitally radiographed for each subject, interpreted and assigned its MP3 stage. Radiodensity was measured for a total sample of 30 radiographs, selected randomly from each pubertal group of MP3 (pre, pubertal, post-pubertal).
Results: The results revealed that there was a statistically significant difference between mean age values at different MP3 growth stages. The mean age value of the peak of pubertal growth spurt represented by MP3 stage G were 11.21 ± 0.85 years old. There was a statistically significant difference between mean bone density values at different pubertal stages (pre-pubertal, pubertal, post-pubertal). Pre-pubertal stage showed the statistically significant lowest mean bone density.
Conclusions: There was a statistically significant difference between mean age values at different MP3 growth stages. The onset and peak of pubertal growth spurt (MP3-FG, MP3-G), in Egyptian girls were found to occur at 10.01 ± 0.91 and 11.21 ± 0.85 years respectively. Accordingly, most of growth modification treatment should be considered according to such age range, which represents the active growth period.
Clinical Relevance:
The (MP3) method is of easy execution and interpretation, and may be performed in any clinical setting with minimal radiation exposure. Therefore, it might be used for planning treatment timing in functional treatments. In addition to close monitoring of the maturity phases by longitudinal recordings.
Development of a Natural Language Processing Pipeline to Identify Histological Subtypes and Site of Cancer from Pathology Reports
Authors: Ng Faith Sze Ee , Low Guat Hwa , Tay See Boon , Tey Han Jieh , Leong Fun Loon , Thng Choon Hua , Tan Iain Bee Huat , Tan Ryan Shea Ying Cong 
Published: October 2022

AbstractPurpose To develop a Natural Language Processing (NLP) pipeline with the ability to determine the histological subtype and site of a patient’s cancer from pathology reports. Methods A Spark NLP-based deep learning model pipeline was developed to perform named entity recognition (NER) and assertion status detection for histological subtypes before extracting key relations of interest to determine the site of a patient’s cancer from pathology reports. We assessed the ability of this NLP pipeline to extract histological subtypes and site of a patient’s cancer against manual curation of pathology reports. Results A total of 1358 reports from 474 patients seen at a single tertiary cancer centre were used in the development and validation of the pipeline. The NLP pipeline achieved a mean accuracy of 99.79% and an F1 score of 84.08% for NER of histological subtypes. The relation extraction (RE) model also achieved an average accuracy of 91.96% and an F1-score of 92.45% for key entity relations relevant to histological subtypes entities. Conclusion We developed an NLP pipeline that can extract the histological subtypes and relate them to the site of a patient’s cancer from free-text pathology reports with high accuracy. This has the potential to be deployed for both research and clinical quality processes.
Supplementation alpha glycerol monolaurate during late gestation and lactation enhanced sow performance, ameliorated milk composition, and improved growth of suckling piglets
Authors: Li Longxian , Wang Huakai , Dong Shuang , Ma Yongxi 
Published: October 2022

Abstract
Background: Physiological changes during lactation cause oxidative stress in sows, reduce immunity, and hamper the growth capacity of piglets. Alpha glycerol monolaurate (α-GML) has potential for improving the health of sows and the growth of suckling piglets.
Methods: Eighty sows were allocated randomly to four groups: basal diet and basal diets supplemented with 500, 1000, or 2000 mg/kg α-GML. The experiment started on d 85 of gestation and lasted until piglets were weaned on d 21 of lactation. The number of live-born piglets was standardized to 12 ± 1 per sow on day of parturition. On d 0 and 21 of lactation, body weight of piglets was measured and milk samples were obtained from sows, and serum samples and feces from piglets were obtained on d 21.
Results: Feed intake, backfat loss, and weaning estrus interval did not differ among the four groups of sows. Maternal α-GML supplementation increased (P < 0.05) the body weight of piglets at weaning. The IgA and IgG levels were greater (P < 0.05) in a quadratic manner in the milk of sows as dietary α-GML increased. Concerning fatty acid profile, C12:0, C15:0, C17:0, C18:2n6c, C18:3n3, C24:0, and C22:6n3 were higher (P < 0.05) in linear and quadratic manners in colostrum of sows-fed α-GML diets compared with the control sows. There was lower (P < 0.05) n-6: n-3 polyunsaturated fatty acid (PUFA) ratio in milk than in the control sows. Maternal α-GML increased the abundance of Firmicutes (P < 0.05) and decreased the abundance of Proteobacteria (P < 0.05) of piglet fecal microbiota.
Conclusions: Dietary supplementation with α-GML improved milk immunoglobulins and fatty acids of sows, thereby improving the health and growth of piglets.
HPLC-DAD Technique for the Quantification of A Recently Approved Anti-diabetic Triple Combination Along with Two Toxic Official Impurities: Toxicity Confirmation Aided by Molecular Docking Application
Authors: Bahgat Eman A. , Hashem Hisham , Saleh Hanaa , Kamel Ebraam B. , Eisaa Maya S. 
Published: October 2022

Abstract
Gliflozins and gliptins are two distinct groups of pharmacological drugs that reduce blood glucose levels in individuals with type II diabetes in various ways that may perform their functions harmoniously. Trijardy® tablet, which contains empagliflozin, linagliptin, and metformin, was recently approved. The scientific database does not yet have a method that is sensitive enough to quantify the aforementioned medications in the presence of metformin official toxic impurities melamine and cyanoguanidine. Molecular docking modeling was utilized in this work to further prove the toxicity of melamine. The five analytes listed before were quantified using RP-HPLC-diode array detector and a Zorbax® C8 column (4.6 × 250 mm, 5 μm) with isocratic mobile phase composed of acetonitrile and 0.05 M potassium dihydrogen phosphate buffer, which had been treated by ο-phosphoric acid to restore a pH of 4.0 (90:10, v/v) at a flow rate of 1.2 mL/min and the eluted peaks were scanned at 250 nm. The utilization of the simplest isocratic elution mode give the current technique a significant time-and cost-saving benefit. The current method can quantify the triple therapy agents in the presence of each other as well as with two official toxic impurities of metformin in one short analytical run.