bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resistance mechanisms and molecular epidemiology of Pseudomonas aeruginosa strains from patients with bronchiectasis
Authors: Cabrera Roberto , Fernández-Barat Laia , Vázquez Nil , Alcaraz-Serrano Victoria , Bueno-Freire Leticia , Amaro Rosanel , López-Aladid Rubén , Oscanoa Patricia , Muñoz Laura , Vila Jordi , Torres Antoni 
Published: March 2022

Abstract

Background
Non-cystic fibrosis bronchiectasis (BE) is a chronic structural lung condition that facilitates chronic colonization by different microorganisms and courses with recurrent respiratory infections and frequent exacerbations. One of the main pathogens involved in BE is Pseudomonas aeruginosa.

Objectives
To determine the molecular mechanisms of resistance and the molecular epidemiology of P. aeruginosa strains isolated from patients with BE.

Methods
A total of 43 strains of P. aeruginosa were isolated from the sputum of BE patients. Susceptibility to the following antimicrobials was analysed: ciprofloxacin, meropenem, imipenem, amikacin, tobramycin, aztreonam, piperacillin/tazobactam, ceftazidime, ceftazidime/avibactam, ceftolozane/tazobactam, cefepime and colistin. The resistance mechanisms present in each strain were assessed by PCR, sequencing and quantitative RT–PCR. Molecular epidemiology was determined by MLST. Phylogenetic analysis was carried out using the eBURST algorithm.

Results
High levels of resistance to ciprofloxacin (44.19%) were found. Mutations in the gyrA, gyrB, parC and parE genes were detected in ciprofloxacin-resistant P. aeruginosa strains. The number of mutated QRDR genes was related to increased MIC. Different β-lactamases were detected: blaOXA50, blaGES-2, blaIMI-2 and blaGIM-1. The aac(3)-Ia, aac(3)-Ic, aac(6″)-Ib and ant(2″)-Ia genes were associated with aminoglycoside-resistant strains. The gene expression analysis showed overproduction of the MexAB-OprM efflux system (46.5%) over the other efflux system. The most frequently detected clones were ST619, ST676, ST532 and ST109.

Conclusions
Resistance to first-line antimicrobials recommended in BE guidelines could threaten the treatment of BE and the eradication of P. aeruginosa, contributing to chronic infection.
Influence of infection with Brachyspira hyodysenteriae on clinical expression, growth performance, and digestibility in growing pigs fed diets varying in type and level of fiber
Authors: Lee Geon Il , Skou Hedemann Mette , Borg Jensen Bent , Bach Knudsen Knud Erik 
Published: March 2022

Abstract
Research on the effects of different fiber types and levels on infection with Brachyspira hyodysenteriae on growth performance and nutrients digestibility in pigs is scarce. The objective of the current study was to investigate the effects of infection with B. hyodysenteriae when feeding diets varying in soluble and insoluble dietary fiber (DF) on the expression of swine dysentery, growth performance, and digestibility of organic matter (OM) nutrients. A total of 96 growing pigs (26.9 ± 2.5 kg) were used for the experiment and divided into six blocks. The growing pigs were fed one of four diets for 12 wk: low fiber (LF), high fiber (HF), high soluble fiber (HS), and high insoluble fiber (HI). After 2 wk, half of the pigs were inoculated with B. hyodysenteriae. Half of the pigs in each group were euthanized at week 6 for the measurement of the apparent digestibility at the ileum, cecum, colon, and total tract. The remaining pigs were maintained to observe and analyze the clinical expression of fecal score and excretion of B. hyodysenteriae, growth performance, and total tract digestibility up to 12 wk. In the current study, the experimental diets did not influence the expression of infection in the pigs. The body weight and average daily gain (ADG) were in line with the results of clinical expression from week 4 to 6. However, the ADG of the infected pigs started to recover from week 6 (P &lt; 0.05) and then recovered from week 8 to 12 (P &lt; 0.05). The infection with B. hyodysenteriae did not impair apparent ileal digestibility (AID; P &gt; 0.05), whereas the apparent digestibility of OM, total non-starch polysaccharide, non-cellulosic polysaccharide, and cellulose in the cecum of the infected pigs was higher than non-infected pigs (P &lt; 0.05). The apparent colonic digestibility of ash and nitrogen was higher in non-infected pigs than in infected pigs (P &lt; 0.05). The pigs fed the LF diet had a higher digestibility in all segments of the intestinal tract, whereas the HS diet had the lowest AID but higher or similar to the LF diet in the cecum, colon, and the total tract (P &lt; 0.05). The pigs fed the HF and HI diets, with a high proportion of insoluble fiber, had a lower digestibility in the hindgut than the other two diets (P &lt; 0.05). In conclusion, the infection with B. hyodysenteriae negatively influenced clinical signs of swine dysentery and growth performance but did not impair AID, and neither soluble nor insoluble DF influenced the expression of the infection.
Comparative Transcriptome Analysis of Superficial and Deep Partial-Thickness Burn Wounds in Yorkshire vs Red Duroc Pigs
Authors: Nguyen Jesse Q , Sanjar Fatemeh , Rajasekhar Karna S L , Fourcaudot Andrea B , Wang Li-Ju , Silliman David T , Lai Zhao , Chen Yidong , Leung Kai P 
Published: March 2022

Abstract
Hypertrophic scars are a common negative outcome of deep partial-thickness (DPT) burn wounds resulting in increased dermal thickness, wound area contracture, and inflammation of the affected area. The red Duroc and Yorkshire porcine breeds are common large animal models for studying dermal wounds due to their structural similarities to human skin; however, the porcine transcriptomic profiles of dermal burn wounds and healing process are not well known. In response, a longitudinal transcriptomic comparative study was conducted comparing red Duroc and Yorkshire superficial and DPT burn wounds to their respective control uninjured tissue. Using next-generation RNA sequencing, total RNAs were isolated from burn wound tissue harvested on 0, 3, 7, 15, 30, and 60 days postburn, and mRNA-seq and gene expression read counts were generated. Significant differentially expressed genes relative to uninjured tissue were defined, and active biological processes were determined using gene set enrichment analyses. Additionally, collagen deposition, α-smooth muscle actin (SMA) protein concentration, epidermal and dermal thickness measurements, and wound area changes in response to burn injury were characterized. Overall, the red Duroc pigs, in response to both burn wound types, elicited a more robust and prolonged inflammatory immune response, fibroblast migration, and proliferation, as well as heightened levels of extracellular matrix modulation relative to respective burn types in the Yorkshire pigs. Collectively, the red Duroc DPT burn wounds produce a greater degree of hypertrophic scar-like response compared with Yorkshire DPT burn wounds. These findings will facilitate future porcine burn studies down-selecting treatment targets and determining the effects of novel therapeutic strategies.
Burn Injuries Associated with At-Home Hair Braiding
Authors: Shakir Afaaf , O’Connor Annemarie , Teele Megan , Gottlieb Lawrence J , Vrouwe Sebastian Q 
Published: March 2022

Abstract
Hair braiding that incorporates synthetic extensions has increased in popularity across all age groups. As part of the styling process, the ends of the braid are commonly dipped in scalding water; an increasing number of patients have presented to our center after containers of recently boiled water are accidentally tipped over and spilled onto patients. A retrospective chart review was performed of all patients who sustained burn injuries related to at-home hair braiding presenting to an ABA-verified burn center between January 1, 2006 and July 31, 2020. A total of 41 patients presented over the study period, and the frequency of this type of burn increased over time, with 54% of injuries occurring in the past 3 years (2018–2020). The mean patient age was 7.5 years, and the majority of patients were under 18 years of age (97%), female (95%), and African-American (98%). Seventy-three percent of injuries occurred in the home and 88% involved another person in the hair braiding process. The mean TBSA burned was 5% (range 1–20%). The most commonly involved areas were the back (54%), thigh/leg (37%), and neck (24%). Ninety percent were partial-thickness injuries only, with 10% having some degree of full-thickness injury. Ninety percent of patients required inpatient admission, and 34% of patients required at least one operative procedure. Hair braiding, with the use of scalding water to seal and set the ends of braids, can lead to burn injuries that require hospitalization and the need for surgical intervention.
Developing an Injury Severity to Yield Loss Relationship for Soybean Gall Midge (Diptera: Cecidomyiidae)
Authors: Helton Mitchell L , Tinsley Nicholas A , McMechan Anthony J , Hodgson Erin W 
Published: March 2022

Abstract
Soybean gall midge, Resseliella maxima Gagné (Diptera: Cecidomyiidae), is a newly identified pest confirmed on soybean, Glycine max (L.) Merr. (Fabales: Fabaceae). To date, soybean gall midge has been found in Nebraska, Iowa, South Dakota, Minnesota, and Missouri, and has caused severe economic loss to commercial fields since 2018. Much is still unknown about this pest, so research efforts have been focused on biology and management. Larvae feed on the inside of the stem just above the soil line and are difficult to access and time-consuming to sample. In order to accelerate nondestructive sampling efforts, we developed an injury rating system to quantify the severity of plant injury from soybean gall midge larvae. Research plots from 2019 and 2020 in Iowa and Nebraska were evaluated for injury throughout the growing season and yield was measured. Our objective was to describe the relationship between injury severity and yield loss caused from soybean gall midge. A nonlinear regression model was developed to validate our injury rating system and to express the relationship between season long injury severity and yield loss. Results from our analysis indicate the injury rating system we developed correlates well with yield loss caused by larvae and may be an important tool for understanding the economic impact of this emergent pest of soybeans.
Influence of Trap Location in the Tree Canopy on Captures of Adventive Trissolcus japonicus (Hymenoptera: Scelionidae)
Authors: Dyer Jared E , Talamas Elijah J , Leskey Tracy C , Bergh J Christopher 
Published: March 2022

Abstract
Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae) is an egg parasitoid of the invasive Asian pest, brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae). Also native to Asia, adventive T. japonicus populations have been detected in North America since 2014, and are currently reported from 15 U.S. states, the District of Columbia, and two Canadian provinces. Yellow sticky cards (YSC) have proven effective for monitoring the presence, seasonal abundance, and distribution of these adventive populations. Our research has utilized YSC deployed in the midcanopy of H. halys host trees, following a study in which all leaves on felled tree of heaven, Ailanthus altissima (Mill.) Swingle, were inspected for H. halys egg masses, yielding eggs parasitized by T. japonicus only from mid- and upper-canopy leaves. However, given that other investigators have captured T. japonicus using YSC deployed in the lower-canopy, and that the effect of YSC placement in trees on T. japonicus captures had not been examined, captures of T. japonicus on YSC in the mid- and lower-canopy of individual A. altissima were compared. Traps were replaced weekly for five weeks and assessed for scelionid species. In 2020 and 2021, T. japonicus represented ≥53% of all Scelionidae captured, and there was not a significant effect of YSC location in the canopy on its captures. Deploying YSC at either canopy height was effective for measuring the relative abundance of T. japonicus, but sampling from the lower canopy substantially improved the efficiency and convenience of T. japonicus surveillance.
Stellar masses of clumps in gas-rich, turbulent disc galaxies
Authors: Ambachew Liyualem , Fisher Deanne B , Glazebrook Karl , Girard Marianne , Obreschkow Danail , Abraham Roberto , Bolatto Alberto , Lenkić Laura , Damjanov Ivana 
Published: March 2022

ABSTRACT
In this paper, we use Hubble Space Telescope/WFC3 observations of six galaxies from the DYnamics of Newly Assembled Massive Object (DYNAMO) survey, combined with stellar population modelling of the SED, to determine the stellar masses of DYNAMO clumps. The DYNAMO sample has been shown to have properties similar to z ≈ 1.5 turbulent, clumpy discs. DYNAMO sample clump masses offer a useful comparison for studies of z &gt; 1 in that the galaxies have the same properties, yet the observational biases are significantly different. Using DYNAMO, we can more easily probe rest-frame near-IR wavelengths and also probe finer spatial scales. We find that the stellar mass of DYNAMO clumps is typically 107−108M⊙. We employ a technique that makes non-parametric corrections in removal of light from nearby clumps, and carries out a locally determined disc subtraction. The process of disc subtraction is the dominant effect, and can alter clump masses at the 0.3 dex level. Using these masses, we investigate the stellar mass function (MF) of clumps in DYNAMO galaxies. DYNAMO stellar MFs follow a declining power law with slope α ≈ −1.4, which is slightly shallower than, but similar to what is observed in z &gt; 1 lensed galaxies. We compare DYNAMO clump masses to results of simulations. The masses and galactocentric position of clumps in DYNAMO galaxies are more similar to long-lived clumps in simulations. Similar to recent DYNAMO results on the stellar population gradients, these results are consistent with simulations that do not employ strong ‘early’ radiative feedback prescriptions.
The study on transmission spectrum and TTV behaviour of the hot Jupiter WASP-12b
Authors: Bai Lu , Gu Shenghong , Wang Xiaobin , Sun Leilei , Kwok Chi-Tai , Hui Ho-Keung 
Published: March 2022

ABSTRACT
By utilizing the 1m and 2.4m telescopes of Yunnan Observatories, we obtained 13 new photometric transit light curves of WASP-12 exoplanetary system from 2009 to 2016. The data were analysed by using the Transit Analysis Package with the aid of Markov chain Monte Carlo technique. Based on the physical parameters from previous work, we have obtained the variation of radius with wavelength and mid-transit times of WASP-12b. Compared with a set of theoretical transmission spectra, its radius variation is almost consistent with a flatten and featureless model along with a slope in the bluer band, except the weak Na signal. The flatten and featureless transmission spectrum implies a possible evidence for the absence of TiO and VO in the upper atmosphere of WASP-12b. The slope in the bluer band may result from the Rayleigh scattering caused by H2/He and there would be hazes or hydride in the upper atmosphere. Combined new mid-transit times with all of the available timing data in literature, we have found that there is a significant change in the planetary orbit, the residuals of the timing prefer the orbital decay model to the apsidal precession model and the planetary interaction-induced TTV model. We updated the decay rate as −37.14 ± 1.31 ms yr−1 and derived a slightly lower modified quality factor $Q^{^{\prime }}_{*}$ for the constant-phase lag model. Furthermore, the current observations cannot rule out the planetary interaction-induced TTV model conclusively.
ATOMS: ALMA Three-millimeter Observations of Massive Star-forming regions – X. Chemical differentiation among the massive cores in G9.62+0.19
Authors: Peng Yaping , Liu Tie , Qin Sheng-Li , Baug Tapas , Liu Hong-Li , Wang Ke , Garay Guido , Zhang Chao , Chen Long-Fei , Lee Chang Won , Juvela Mika , Li Dalei , Tatematsu Ken’ichi , Liu Xun-Chuan , Lee Jeong-Eun , Luo Gan , Dewangan Lokesh , Wu Yue-Fang , Zhang Li , Bronfman Leonardo , Ge Jixing , Tang Mengyao , Zhang Yong , Xu Feng-Wei , Wang Yao , Zhou Bing 
Published: March 2022

ABSTRACTInvestigating the physical and chemical structure of massive star-forming regions is critical for understanding the formation and early evolution of massive stars. We performed a detailed line survey toward six dense cores, named MM1, MM4, MM6, MM7, MM8, and MM11, in the G9.62+0.19 star-forming region resolved in Atacama Large Millimeter/submillimeter Array (ALMA) band 3 observations. Toward these cores, about 172 transitions have been identified and attributed to 16 species, including organic oxygen-, nitrogen-, and sulphur-bearing molecules and their isotopologues. Four dense cores, MM7, MM8, MM4, and MM11, are line-rich sources. Modelling of these spectral lines reveals that the rotational temperature lies in the range 72–115, 100–163, 102–204, and 84–123 K for MM7, MM8, MM4, and MM11, respectively. The molecular column densities are 1.6 × 1015–9.2 × 1017 cm−2 toward the four cores. The cores MM8 and MM4 show a chemical difference between oxygen- and nitrogen-bearing species, i.e. MM4 is rich in oxygen-bearing molecules, while nitrogen-bearing molecules, especially vibrationally excited HC3N lines, are mainly observed in MM8. The distinct initial temperatures at the accretion phase may lead to this N/O differentiation. Through analysing column densities and spatial distributions of O-bearing complex organic molecules (COMs), we found that C2H5OH and CH3OCH3 might have a common precursor, CH3OH. CH3OCHO and CH3OCH3 are likely chemically linked. In addition, the observed variation in HC3N and HC5N emission may indicate their different formation mechanisms in hot and cold regions.
Metallicity estimation of core-collapse Supernova H iiregions in galaxies within 30 Mpc
Authors: Ganss R , Pledger J L , Sansom A E , James P A , Puls J , Habergham-Mawson S M 
Published: March 2022

ABSTRACTThis work presents measurements of the local H ii environment metallicities of core-collapse supernovae (SNe) within a luminosity distance of 30 Mpc. 76 targets were observed at the Isaac Newton Telescope and environment metallicities could be measured for 65 targets using the N2 and O3N2 strong emission line method. The cumulative distribution functions (CDFs) of the environment metallicities of Type Ib and Ic SNe tend to higher metallicity than Type IIP, however Type Ic are also present at lower metallicities whereas Type Ib are not. The Type Ib frequency distribution is narrower (standard deviation ∼0.06 dex) than the Ic and IIP distributions (∼0.15 dex) giving some evidence for a significant fraction of single massive progenitor stars; the low metallicity of Type Ic suggests a significant fraction of compact binary progenitors. However, both the Kolmogorov–Smirnov test and the Anderson–Darling test indicate no statistical significance for a difference in the local metallicities of the three SN types. Monte Carlo simulations reveal a strong sensitivity of these tests to the uncertainties of the derived metallicities. Given the uncertainties of the strong emission methods, the applicability of the tests seems limited. We extended our analysis with the data of the Type Ib/Ic/IIP SN sample from Galbany et al. The CDFs created with their sample confirm our CDFs very well. The statistical tests, combining our sample and the Galbany et al. sample, indicate a significant difference between Type Ib and Type IIP with &lt;5 per cent probability that they are drawn from the same parent population.
Properties of the Be-type stars in 30 Doradus
Authors: Dufton P L , Lennon D J , Villaseñor J I , Howarth I D , Evans C J , de Mink S E , Sana H , Taylor W D 
Published: March 2022

ABSTRACTThe evolutionary status of Be-type stars remains unclear, with both single-star and binary pathways having been proposed. Here, VFTS spectroscopy of 73 Be-type stars, in the spectral-type range, B0–B3, is analysed to estimate projected rotational velocities, radial velocities, and stellar parameters. They are found to be rotating faster than the corresponding VFTS B-type sample but simulations imply that their projected rotational velocities are inconsistent with them all rotating at near critical velocities. The de-convolution of the projected rotational velocities estimates leads to a mean rotational velocity estimate of 320–350 km s−1, approximately 100 km s−1 larger than that for the corresponding B-type sample. There is a dearth of targets with rotational velocities less than 0.4 of the critical velocity, with a broad distribution reaching up to critical rotation. Our best estimate for the mean or median of the rotational velocity is 0.68 of the critical velocity. Rapidly rotating B-type stars are more numerous than their Be-type counterparts, whilst the observed frequency of Be-type stars identified as binary systems is significantly lower than that for normal B-type stars, consistent with their respective radial-velocity dispersions. The semi-amplitudes for the Be-type binaries are also smaller. Similar results are found for a Small Magellanic Cloud Be-type sample centred on NGC 346 with no significant differences being found between the two samples. These results are compared with the predictions of single and binary stellar evolutionary models for Be-type stars. Assuming that a single mechanism dominated the production of classical Be-type stars, our comparison would favour a binary evolutionary history.
The flaring TESS Objects of Interest: flare rates for all two-minute cadence TESS planet candidates
Authors: Howard Ward S 
Published: March 2022

ABSTRACT
Although more than 5000 TESS Objects of Interest have been catalogued, no comprehensive survey of the flare rates of their host stars exists. We perform the first flare survey of all 2250 non-retired TOIs with 2-min cadence light curves to measure or place upper limits on their flare rates. We find 93 candidates orbit flare stars and measure their flare frequency distributions. Across the sample, TOIs of ≤1.5R⊕ orbit flare stars more frequently than do TOIs of 1.5 &lt; R&lt; 2.75R⊕, 2.75 &lt; R&lt; 4R⊕, or R≥ 4R⊕. We sort all TOI host stars by their flare rate/upper limit, stellar mass, and distance to create a flare ranking metric (FRM) to determine suitability for follow-up. The FRM of each TOI is then checked against the expected signal-to-noise of atmospheric features in transmission spectroscopy to locate the most promising targets. We find 1/4 of terrestrial M-dwarf planets amenable to transmission spectroscopy orbit flare stars. However, none of the M-dwarf hosts to terrestrial planets are currently flaring at sufficient levels for &gt;99.9 per cent atmospheric ozone depletion. We give the first upper limits on the flare rate of the host star to TOI 700 d and explore the flare rates incident on young planets such as DS Tuc Ab.
Refractory alopecia universalis associated with dermatomyositis successfully treated with tofacitinib
Authors: Castillo Rochelle , Albayda Jemima 
Published: March 2022

ABSTRACT
Dermatomyositis (DM) and alopecia areata are two diseases characterised by aberrant interferon signalling. While patchy alopecia of the scalp is a known feature of DM, alopecia universalis, which involves hair loss over the entire body, has rarely been reported in conjunction with DM. Herein, we report the case of a 30-year-old female with DM who developed refractory cutaneous disease and alopecia universalis that were successfully treated with tofacitinib. This could suggest that concomitant severe alopecia and refractory cutaneous DM may reflect a strong baseline interferon gene signature that may predict responsiveness to janus kinase inhibitors.