bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSII-9 Preliminary Investigation of Nutrient Challenge During Pregnancy on Wether Lamb Development, Growth, and Performance from Birth to Harvest
Authors: Kuderka Alison , Vautier Allison N , Gallop Sally , Archibeque Shawn L , Cadaret Caitlin N 
Published: October 2022

Abstract
Inadequate nutrition during gestation has been shown to impair growth and metabolism of affected offspring and can predispose them to lifelong inefficiency and poor performance. Development throughout preweaning has been studied and compared to carcass performance; however, the impact of nutrient challenge during gestation on post weaning growth, efficiency, and nutrition utilization is not well known. Thus, our objective was to investigate the impacts of maternal nutrient restriction in late gestation on wether lambs' lifelong performance, from birth through harvest. We hypothesized that lambs from nutrient restricted dams would have reduced growth efficiency, taking longer to reach market weight and yielding altered body and carcass composition. Twenty-two bred yearling ewes were fed a diet meeting 100% of National Research Council (NRC) requirements until the 110th day of gestion (dGA). From 110 dGA until lambing occurred, ewes were either maintained on a diet meeting 100% of NRC requirements (n = 10) or reduced to a diet of 65% of NRC requirements (n = 12). Beginning at birth, body weight and morphometrics were collected weekly for control (CONT; n = 4) and treatment (NR; n = 5) wether lambs. At approximately 71 days of age, wethers were weaned and placed into individual pens to simulate a controlled feedlot setting. Wether lambs were fed a commercial lamb finishing diet ad libitum and orts were collected to evaluate feed efficiency and growth. At 115 days of age, wethers were placed in metabolism crates for 5 days to allow for total collection or urine and feces in order to evaluate nutrient balance. Birthweight, weaning weight, and morphometrics did not differ between NR and CONT lambs from birth until weaning. However, there was a tendency (P &lt; 0.10) for a slower rate of gain from birth until weaning in NR lambs compared to their CONT counterparts. Current research in feedlot performance and digestibility is ongoing; however, thus far there are no differences in feed intake or average daily gain. These data suggest that a moderate nutrient challenge in the last month of gestation is not substantial enough to induce adaptive changes that result in differences in postnatal growth and performance.
PSIII-1 Late-Breaking: Fighting Fetal Morbidity at the Fetal-Maternal Interface One Chemokine Access at a Time
Authors: Mason Kaitlyn M , Ashley Ryan L 
Published: October 2022

Abstract
Improper placentation is the root cause to intrauterine growth restriction (IUGR) and fetal mortality in livestock. This study aims to further characterize the importance of the C-X-C motif chemokine receptor 4 (CXCR4) and its ligand CXCL12 on placentation and fetal growth through potential impacts on the insulin-like growth factors (IGF) and their binding proteins (IGFBP1-3). CXCR4 and CXCL12 are implicated in placentation though the exact roles are unclear. The peptides insulin-like growth factors 1 and 2 have impacts on fetal growth, placental development, and trophoblast differentiation and if the IGFs are down regulated, a corresponding decrease in placenta and fetus size has been observed. The roles of the IGFs and their binding proteins in disease states like IUGR are not completely known but studies have demonstrated them to be contributing factors. Using an in-vivo sheep model we surgically inserted osmotic pumps on day 12 (d12) of gestation ipsilateral to the corpus luteum to deliver treatments into the uterus of bred ewes. Pumps delivered either saline (control) or AMD3100, the CXCR4 inhibitor at a 1x, 1.5x, or 3x dose into the uterine lumen over the course of 14 days. Our objective was to determine if disrupting the CXCL12/CXCR4 axis during implantation alters the expression of IGFs and their binding proteins in the placenta at mid and late gestation. At midgestation (d90) and late gestation (d135) placental and fetal samples were obtained for analysis. Of the targets tested, mRNA expression was significantly decreased by AMD3100 treatment for both IGF-1 (P = 0.05) and IGF-2 (P = 0.02) compared to control primarily in maternal placenta tissues on d135. For fetal tissue samples, d90 mRNA expression for IGF-1 (P = 0.04) IGFBP-2 (P = 0.02) and IGFBP-3 (P = 0.03) increased in the 3x AMD3100 treated group compared to the control while in d135 samples, IGF-1was significantly decreased (P = 0.04) by the 1x treatments compared to control. Our preliminary data show an importance of the CXCL12- CXCR4 signaling on placentation and that impairment of this axis may alter IGFs in multiple tissue types later in gestation. Insights into this chemokine axis will lead to a greater understanding of placental development and its importance to the health of mother and offspring.
Technical note: an optimized method to isolate, purify, and differentiate satellite cells from broiler chicks
Authors: Jung Usuk , Kim Minjeong , Piacquadio Kamille , Shepherd Elizabeth , Voy Brynn H 
Published: October 2022

Abstract
Development and maintenance of healthy muscle fibers rely on the myogenic potential of satellite cells (SC), muscle stem cells that proliferate and differentiate to form myotubes. Satellite cells are indispensable for post-hatch muscle growth as well as muscle repair and regeneration when myofibers are damaged. Pectoralis major of young broiler chicks (5-d olds) is a readily available source of SC, which can be used in vitro to elucidate cellular and molecular mechanisms responsible for muscle growth and regeneration in broilers. Here, we optimized a method for efficient isolation, purification, and differentiation of SC, from young broiler chicks. This procedure includes a simple method that allows SC to be purified from other muscle cell types that can impede the fidelity of follow-on experiments, particularly highly sensitive measures such as RNAseq. The methods for culturing and differentiating SC into multinucleated myotubes were also optimized by testing serum types, concentrations, and the effects of chicken embryo extract. Using the isolation procedure, a highly pure SC population (94.6 ± 2.11% Pax7+) with high viability and yield was obtained, and their capacity to differentiate into myotubes was confirmed. Enrichment for SC and myogenic capacity were maintained through multiple passages and after cryopreservation. Analysis of gene expression over the first 48 h of differentiation confirmed that SC exhibited the expected molecular signature of myogenesis. Taken together, this method simplifies the ability to isolate and maintain a relatively pure population of SC with strong myogenic potential from young broiler chicks, and should support downstream applications for assessing the impact of nutrients, metabolites, and other physiological cues on muscle growth and development in broilers.
Dietary supplementation of botanical blends enhanced performance and disease resistance of weaned pigs experimentally infected with enterotoxigenic Escherichia coli F18
Authors: Wong Braden T , Park Sangwoo , Kovanda Lauren , He Yijie , Kim Kwangwook , Xu Shiyu , Lingga Christopher , Hejna Monika , Wall Emma , Sripathy Ravichandran , Li Xunde , Liu Yanhong 
Published: October 2022

Abstract
Botanicals exhibit promising impacts on intestinal health, immune-regulation, and growth promotion in weaned pigs. However, these benefits may vary depending on major active components in the final feed additive products. Therefore, this study aimed to investigate two types of botanical blends (BB) that were comprised of 0.3% capsicum oleoresin and 12% garlic extracts from different sources on performance, diarrhea, and health of weaned piglets experimentally infected with a pathogenic Escherichia coli F18. Sixty weanling pigs (7.17 ± 0.97 kg body weight (BW)) blocked by weight and gender were assigned to one of five dietary treatments: negative control (NC), positive control (PC), or dietary supplementation with 100 mg/kg of BB1, 50 mg/kg or 100 mg/kg of BB2. This study lasted 28 d with 7 d before and 21 d after the first E. coli inoculation (day 0). All pigs, except negative control, were orally inoculated with 1010 cfu E. coli F18/3-mL dose for 3 consecutive days. Blood samples were collected periodically to analyze systemic immunity. Intestinal tissues and mucosa were collected on days 5 and 21 PI for analyzing histology and gene expression. All data, except for frequency of diarrhea, were analyzed by ANOVA using the PROC MIXED of SAS. The Chi-square test was used for analyzing frequency of diarrhea. Escherichia coli infection reduced (P &lt; 0.05) growth rate and feed intake and increased (P &lt; 0.05) frequency of diarrhea of weaned pigs throughout the experiment. Supplementation of 100 mg/kg BB1 or BB2 alleviated (P &lt; 0.05) frequency of diarrhea of E. coli challenged pigs during the entire experiment. Escherichia coli infection also enhanced (P &lt; 0.05) serum TNF-α and haptoglobin concentrations on day 4 post-inoculation (PI) but reduced (P &lt; 0.05) duodenal villi height and area on day 5 PI, while pigs supplemented with 100 mg/kg BB1 or BB2 had lower (P &lt; 0.05) serum TNF-α than pigs in PC on day 4 PI. Pigs fed with 100 mg/kg BB2 had higher (P &lt; 0.05) jejunal villi height than pigs in PC on day 5 PI. Pigs fed with 100 mg/kg BB2 had reduced (P &lt; 0.05) gene expression of IL1B, PTGS2, and TNFA in ileal mucosa than pigs in PC on day 21 PI. In conclusion, dietary supplementation of botanical blends at 100 mg/kg could enhance disease resistance of weaned pigs infected with E. coli F18 by enhancing intestinal morphology and regulating local and systemic immunity of pigs.
Evaluation of graded levels of corn-fermented protein on stool quality, apparent nutrient digestibility, and palatability in healthy adult cats
Authors: Kilburn-Kappeler Logan R , Lema Almeida Krystina A , Aldrich Charles G 
Published: October 2022

Abstract
Dried distillers’ grains, coproducts from the ethanol industry, may provide sustainable ingredients for pet food. Due to new post-fermentation separation techniques, corn-fermented protein (CFP) is higher in protein and lower in fiber compared with traditional dried distillers’ grains, increasing its appeal for inclusion into pet food. Therefore, the objectives of this study were to determine the effects of increasing levels of CFP on stool quality, apparent total tract digestibility (ATTD), and palatability in adult cats. Four extruded diets were fed to 11 adult cats in an incomplete 4 × 4 replicated Latin square design. The control diet contained 15% soybean meal (0C) and CFP was exchanged for soybean meal at either 5%, 10%, or 15% (5C, 10C, 15C). Cats were fed each dietary treatment for 9-d adaption followed by 5-d total fecal collection. Feces were scored on a 1 to 5 scale, with 1 representing liquid diarrhea and 5 representing hard pellet-like (Carciofi et al., 2008). A fecal score of 3.5 to 4 was considered ideal. Titanium dioxide was added to all diets (0.4%) as a marker to estimate digestibility. Data were analyzed using a mixed model in SAS (version 9.4, SAS Institute, Inc., Cary, NC) with treatment as a fixed effect and cat and period as random effects. Fecal dry matter percent and dry fecal output were greater (P &lt; 0.05) at elevated levels of CFP. Stool scores were maintained (P &gt; 0.05) throughout treatments (average; 4). Dry matter, organic matter, crude protein, and gross energy ATTD decreased when cats were fed 15C. There was no difference in ATTD of fat or total dietary fiber among treatments. For palatability assessment, cats preferred 5C over 0C but had no preference with increased CFP inclusion. These results suggest that CFP is comparable to SBM, but there may be a maximum inclusion level of 10% when fed to cats.
Calmangafodipir for Prevention of Oxaliplatin-Induced Peripheral Neuropathy: Two Placebo-Controlled, Randomized Phase 3 Studies (POLAR-A/POLAR-M)
Authors: Pfeiffer Per , Lustberg Maryam , Näsström Jacques , Carlsson Stefan , Persson Anders , Nagahama Fumiko , Cavaletti Guido , Glimelius Bengt , Muro Kei 
Published: October 2022

Abstract

Background
Calmangafodipir (CaM, PledOx) demonstrated efficacy in preventing patient-reported chemotherapy-induced peripheral neuropathy (CIPN) in a randomized phase 2 study in patients with metastatic colorectal cancer. The Preventive Treatment of OxaLiplatin Induced peripherAl neuRopathy (POLAR) program aimed to assess efficacy and safety of CaM in the prevention of CIPN in patients treated with oxaliplatin in adjuvant (POLAR-A, ClinicalTrials.gov.NCT04034355) or metastatic (POLAR-M, ClinicalTrials.gov.NCT03654729) settings.

Methods
Two randomized, placebo-controlled phase 3 trials investigated patient-reported, moderate-to-severe CIPN 9 months after beginning folinic acid, 5-fluorouracil, and oxaliplatin therapy with or without CaM. In POLAR-A, patients with stage III or high-risk stage II colorectal cancer were randomly assigned 1:1 to receive CaM 5 μmol/kg or placebo. In POLAR-M, patients with metastatic colorectal cancer were randomly assigned 1:1:1 to receive CaM 5 μmol/kg, CaM 2 μmol/kg, or placebo.

Results
POLAR-A (n = 301) and POLAR-M (n = 291) were terminated early following unexpected hypersensitivity reactions in CaM-treated patients. In a combined analysis of month 9 CIPN (primary endpoint) data from both trials (CaM 5 μmol/kg, n = 175; placebo, n = 176), 54.3% of patients in the CaM group had moderate-to-severe CIPN compared with 40.3% in the placebo group. The estimated relative risk for moderate-to-severe CIPN at month 9 was 1.37 (95% confidence interval = 1.01 to 1.86; P = .045). A higher proportion of patients experienced serious hypersensitivity reactions across both trials with CaM treatment (3.6%) than with placebo (0.8%).

Conclusion
The POLAR clinical studies failed to meet their primary endpoint. These results highlight the challenges of targeting oxidative stress for preventing CIPN in both the adjuvant and metastatic settings.
Heat tracer-based sap flow methods for tree transpiration measurements: a mini review and bibliometric analysis
Authors: Wang Jiaming , Turner Neil C , Feng Hao , Dyck Miles , He Hailong 
Published: October 2022

AbstractAccurate measurement of plant transpiration is critical to gaining a better understanding of plant water use and exploration of the influence of plants on regional and even global climate. Heat tracer-based sap flow (HTSF) techniques are currently the dominant method to estimate plant transpiration at the individual plant level. However, the majority of current research focuses on specific applications or the evaluation of the method itself, and there is a lack of an overall analysis of HTSF methods. The objectives of this study were: (i) to briefly review the theories and categories of the various HTSF methods, and (ii) to undertake a bibliometric analysis of the use of HTSF methods in measuring plant transpiration. Each HTSF method is described mathematically and their application and pros and cons are briefly discussed. A bibliometric analysis was conducted using 3964 papers published between 1992 and 2020 archived in the Web of Science core collection. The analysis identified publication trends, the most productive authors, organizations, and countries, as well as the most utilized HTSF method (i.e., thermal dissipation) and journals in which these papers were published. In addition, world distribution maps of the use of HTSF methods and tree species measured were drawn based on 741 selected publications with in situ measurements.
Military Healthcare Ethics: Making It Relevant to the Whole Military Care Team
Authors: Lin Chiu-Yi , Bricknell Martin C M , Brockie Alan F , Kelly Janet Clair 
Published: October 2022

ABSTRACT
This article notes the significant increase in academic papers and policy guidance on the subject of ethical practice in military healthcare over the past two decades. This is usually within the domain of “military medical ethics,” linking medical ethics as applied to the medical profession (doctors) with ethics as applied within the military (primarily from the perspective of officers). This article argues that this, highly elitist, perspective disenfranchises the majority of the military healthcare team who are nurses and allied health professionals and serve across the entire rank spectrum. We suggest that the subject should be reframed under the banner “military healthcare ethics” to include the concepts within military medical ethics but to emphasize the obligations of all military health professionals to comply with legal, regulatory, and ethical guidance for the practice of healthcare in the military environment. We recommend that the subject should be included in the curricula for education and training for all military health professions across their whole career.
Detection rate of fast radio bursts in the Milky Way with BURSTT
Authors: Ling Decmend Fang-Jie , Hashimoto Tetsuya , Yamasaki Shotaro , Goto Tomotsugu , Kim Seong Jin , Ho Simon C-C , Hsiao Tiger Y-Y , Wong Yi Hang Valerie 
Published: October 2022

ABSTRACT
Fast radio bursts (FRBs) are intense bursts of radio emission with durations of milliseconds. Although researchers have found them happening frequently all over the sky, they are still in the dark to understand what causes the phenomena because the existing radio observatories have encountered certain challenges during the discovery of FRB progenitors. The construction of Bustling Universe Radio Survey Telescope in Taiwan (BURSTT) is being proposed to solve these challenges. We simulate mock Galactic FRB-like events by applying a range of spatial distributions, pulse widths, and luminosity functions (LFs). The effect of turbulent interstellar medium (ISM) on the detectability of FRB-like events within the Milky Way plane is considered to estimate the dispersion measure and pulse scattering of mock events. We evaluate the fraction of FRB-like events in the Milky Way that are detectable by BURSTT and compare the result with those by Survey for Transient Astronomical Radio Emission 2 (STARE2) and Galactic Radio Explorer (GReX). We find that BURSTT could increase the detection rate by more than two orders of magnitude compared with STARE2 and GReX, depending on the slope of LF of the events. We also investigate the influence of the specifications of BURSTT on its detection improvement. This leads to the fact that greatly higher sensitivity and improved coverage of the Milky Way plane have significant effects on the detection improvement of BURSTT. We find that the upgraded version of BURSTT, BURSTT-2048 could increase the detection rate of faint Galactic FRB-like events by a factor of 3.
Truncated accretion discs in black hole X-ray binaries: dynamics and variability signatures
Authors: Dihingia Indu K , Vaidya Bhargav , Fendt Christian 
Published: October 2022

ABSTRACT
Variable features in black hole X-ray binaries (BH-XRBs) are observed in different energy ranges and time-scales. The physical origin of different spectral states in BH-XRBs and their relations with the underlying accretion disc are still elusive. To investigate the intermediate state of BH-XRBs during outburst, we simulate a truncated accretion disc around a Kerr black hole using a general relativistic magnetohydrodynamical (GRMHD) framework under axisymmetry with adaptively refined mesh. Additionally, we have also carried out radiative transfer calculations for understanding the implications of disc dynamics on emission. Dynamically, the inner edge of the truncated accretion disc oscillates in a quasi-periodic fashion (QPO). The QPO frequency of oscillations (νQPO, max) increases as the magnetic field strength and magnetic resistivity increase. However, as the truncation radius increases, νQPO, max decreases. In our simulation models, frequency varies between $7\times (10\, {\rm M}_{\odot }/M_{\rm BH})$ Hz $\lesssim \nu _{\rm QPO, max}\lesssim 20 \times (10\, {\rm M}_{\odot }/M_{\rm BH})$ Hz, which is in the range of low-frequency QPOs. We further find evidence of transient shocks in the highly accreting stage during oscillation. Such a transient shock acts as an extended hot post-shock corona around the black hole that has an impact on its radiative properties. The radiative transfer calculations show signatures of these oscillations in the form of modulation in the edge-brightened structure of the accretion disc.
Forming stars in a dual AGN host: molecular and ionized gas in the nearby, luminous infrared merger, Mrk 266
Authors: Beaulieu Damien , Petric Andreea , Robert Carmelle , Alatalo Katherine , Heckman Timothy , Merhi Maya , Rousseau-Nepton Laurie , Rowlands Kate 
Published: October 2022

ABSTRACT
We present star formation rates based on cold and ionized gas measurements of Mrk 266 (NGC 5256), a system composed of two colliding gas-rich galaxies, each hosting an active galactic nucleus. Using 12CO (1-0) observations with the Combined Array for Research in Millimeter-Wave Astronomy (CARMA), we find a total H2 mass in the central region of 1.1 ± 0.3 × 1010 M⊙ which leads to a possible future star formation rate of 25 ± 10 M⊙ yr−1. With the Fourier Transform Spectrograph (SITELLE) on the Canada–France–Hawaii Telescope, we measure an integrated H α luminosity and estimate a present-day star formation rate of 15 ± 2 M⊙ yr−1 in the core of the system (avoiding the two active nuclei). These results confirm that Mrk 266 is an intermediate stage merger with a relatively high recent star formation rate and enough molecular gas to sustain it for a few hundred million years. Inflowing gas associated with the merger may have triggered both the starburst episode and two active galactic nuclei but the two galaxy components differ: the region around the south-western nucleus appears to be more active than the north-east nucleus, which seems relatively quiet. We speculate that this difference may originate in the properties of the interstellar medium in the two systems.
Gravitational scattering of the sterile neutrino halo dark matter
Authors: Chan Man Ho 
Published: October 2022

ABSTRACT
A recent study shows that gravitational scattering of dark matter, in the form of massive objects with mass m ∼ 103–104M⊙, could provide a possible solution to alleviate the small-scale structure problems of cold dark matter. The scattering cross-section is velocity-dependent so that this scenario can explain why self-interaction of dark matter is significant in dwarf galaxies, but not in massive galaxies and galaxy clusters. In this Letter, we show that this kind of dark massive objects could be made of sterile neutrinos with a possible rest mass range mν ∼ 7.6 keV–71 MeV. This mass range generally satisfies most of the current observational constraints. The entire structure of the sterile neutrino haloes can be simply predicted from standard physics.