bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of TDP‐43 Splicing Repression Occurs in Myonuclei of Inclusion Body Myositis Patients
Authors: Ikenaga Chiseko , Wilson Andrew B. , Irwin Katherine E. , Peethambaran Mallika Aswathy , Kilgore Collin , Sinha Irika R. , Michelle Elizabeth H. , Ling Jonathan P. , Wong Philip C. , Lloyd Thomas E. 
Published: January 2025

ObjectiveInclusion body myositis (IBM) is an idiopathic inflammatory myopathy with muscle pathology characterized by endomysial inflammation, rimmed vacuoles, and cytoplasmic mislocalization of transactive response DNA‐binding protein 43 (TDP‐43). We aimed to determine whether loss of TDP‐43 splicing repression led to the production of “cryptic peptides” that could be detected in muscle biopsies as a useful biomarker for IBM.MethodsWe used an antisera against a neoepitope encoded by a TDP‐43‐dependent cryptic exon within hepatoma‐derived growth factor‐like protein 2 (HDGFL2) for immunohistochemical analysis on muscle biopsy samples of 122 patients with IBM, 181 disease controls, and 16 healthy controls without abnormal muscle pathology. In situ hybridization was also utilized to detect the localization of cryptic HDGFL2 transcripts.ResultsWe found cryptic HDGFL2 peptides localized within myonuclei from muscle biopsies in 79 of 122 patients with IBM (65%), and this staining correlated with TDP‐43 depletion. In contrast, cryptic HDGFL2 immunoreactivity was absent in 197 muscle biopsies from a variety of disease controls, except for 2 patients with vacuolar myopathies. Notably, we show that cryptic HDGFL2 transcripts are accompanied by the detection of cryptic HDGFL2 in muscle fibers of IBM without rimmed vacuoles and TDP‐43 aggregates.InterpretationTogether, our findings establish that loss of TDP‐43 splicing repression occurs in myonuclei of IBM skeletal muscle and suggest that detection of cryptic peptides in muscle biopsies may be a useful biomarker. We suggest that a therapeutic strategy designed to restore TDP‐43 function should be considered to attenuate the degeneration of skeletal muscle in this devastating disease. ANN NEUROL 2025
CO2‐Driven Oxygen Vacancy Diffusion and Healing on TiO2(110) at Ambient Pressure
Authors: Kim Young Jae , Choi Hyuk , Kim Daeho , Kim Yongman , Kim Ki‐jeong , Kim Jeongjin , Thornton Geoff , Kim Hyun You , Park Jeong Young 
Published: January 2025

AbstractUnderstanding how TiO2 interacts with CO2 at the molecular level is crucial in the CO2 reduction toward value‐added energy sources. Here, we report in situ observations of the CO2 activation process on the reduced TiO2(110) surface at room temperature using ambient pressure scanning tunneling microscopy. We find that oxygen vacancies (Vo) diffuse dynamically along the bridging oxygen (Obr) rows of the TiO2(110) surface under ambient CO2(g) environments. This physical phenomenon exclusively occurs when the oxygen abstracted upon CO2 dissociation instantly occupies the Vo sites of Obr rows on the TiO2(110), whereas the TiO2(110) surface without the Vo only allows CO2 physisorption on five‐fold‐coordinated Ti4+ sites. Synchrotron‐based ambient pressure X‐ray photoelectron spectroscopy also identifies the changes in surface oxidation states of TiO2(110) by the healing of Vo sites or the CO2 physisorption under ambient CO2(g) conditions. Density functional theory calculations propose a mechanism of the CO2‐driven Vo diffusion and the physisorbed CO2 configurations. Our combined results unravel the critical role of defect sites on TiO2 in determining the elementary step of CO2 activation during chemical reactions.
A Zero‐Gap Electrolyzer Enables Supporting Electrolyte‐Free Seawater Splitting for Energy‐Saving Hydrogen Production
Authors: Ren Yongwen , Fan Faying , Zhang Shu , Liu Zhaohan , Zhang Yaojian , Sun Fu , Li Jiedong , Chen Lin , Wang Zhe , Zhao Jingwen , Qiu Jieshan , Cui Guanglei 
Published: January 2025

AbstractMembrane‐assisted direct seawater splitting (DSS) technologies are actively studied as a promising route to produce green hydrogen (H2), whereas the indispensable use of supporting electrolytes that help to extract water and provide electrochemically‐accelerated reaction media results in a severe energy penalty, consuming up to 12.5 % of energy input when using a typical KOH electrolyte. We bypass this issue by designing a zero‐gap electrolyzer configuration based on the integration of cation exchange membrane and bipolar membrane assemblies, which protects stable DSS operation against the precipitates and corrosion in the absence of additional supporting electrolytes. The heterolytic water dissociation function of the bipolar membrane in situ creates an asymmetric acidic‐alkaline environment, kinetically facilitating H2 and O2 evolution reactions. When working in natural seawater without any chemical inputs, this zero‐gap electrolyzer sustains nearly 100 % Faradaic efficiency toward H2 for 120 h at a current density of 100 mA cm−2. With the high‐integrity merit, our electrolyzer can be facilely scaled up into practical cell stacks with significantly increased active area and promising prospects for volume/space‐sensitive application scenarios. This electrolyzer concept opens an underexplored design space for energy‐saving H2 production from low‐grade saline water sources, being complementary to, and potentially competitive with the pre‐purification Schemes.
Evolution of Morphological, Electrical, Thermal, and Mechanical Properties of Elastomer Nanocomposites With Different Sizes of MWCNTs
Authors: Evgin Tuba , Micusik Matej , Koca Halil Doğacan , Peidayesh Hamed , Turgut Alpaslan , Slouf Miroslav , Chodak Ivan 
Published: January 2025

ABSTRACTEthylene‐propylene‐diene‐monomer (EPDM) nanocomposites with three various types of multiwall carbon nanotubes (MWCNTs) were fabricated to investigate the size effect of MWCNTs on the morphological, electrical, thermal, mechanical, and viscoelastic properties, as well as swelling ratio and crosslinking density. MWCNTs enhance electrical, thermal, mechanical, and viscoelastic properties regardless of MWCNT size. The MWCNT size is vital in determining percolation thresholds and thermal conductivity; however, it considerably influences the electrical conductivity in only the percolation threshold region (2–10 phr). In addition, the diameter of MWCNTs is a more regulating factor for nanocomposites' electrical properties than length. The EPDM nanocomposites, including MWCNTs with a longer length, low diameter, and higher specific surface area (SSA), show a better thermal conductivity enhancement (95%) due to forming more easily thermally conductive networks. The size of MWCNTs is a negligible key factor for mechanical and viscoelastic properties. With increasing MWCNT contents, the swelling ratio decreases, and crosslinking density increases. MWCNTs with lower diameter, length, and higher SSA exhibit less enhancement in all mentioned properties of EPDM‐based nanocomposites due to worse dispersion in the matrix.
Composite nonlinear feedback event‐triggered control of switched system with input saturation
Authors: Wang Juan , Li Xiaohu , Zhu Shuaidong , Zhu Quanmin 
Published: January 2025

AbstractThis paper focuses on the composite nonlinear feedback (CNF) event‐triggered control of saturated switched systems. The proposed CNF event‐triggered control method can not only improve transient performance of systems but also decrease network bandwidth consumption. Here it is worth emphasizing that this event‐triggered mechanism is presented based on the inherent characteristics of CNF control and switched systems. Whether to sample the current state of system is determined by the specialized event‐triggered condition, and under this triggering condition, we obtain the practical stability of saturated switched systems via multiple Lyapunov functions method. Switching signal satisfies dwell time constraint. The proposed method can avoid Zeno behavior, and the specific proof has been provided. In addition, an example of turbofan engine model verifies the feasibility and effectiveness of the given method.
On the Feasibility of Pairing Pyrochlore Iron(III) Hydroxy Fluoride Cathode with Argyrodite Li6PS5Cl Solid‐State Electrolyte for Low‐Cost All‐Solid‐State Batteries
Authors: Sivavec Jaka , Baumgärtner Julian F. , Stoian Dragos C. , Klimpel Matthias , van Beek Wouter , Kovalenko Maksym V. , Kravchyk Kostiantyn V. 
Published: January 2025

AbstractAs demand for low‐cost, high‐energy‐density all‐solid‐state batteries continues to rise, exploring novel cathodes composed of earth‐abundant elements is imperative. Iron hydroxy fluorides with the pyrochlore structure (Pyr‐IHF) emerge as compelling cathode materials due to abundant natural reserves of their constituent elements, high energy density, and rate capability. In this work, we explore the viability of Pyr‐IHF as a cathode material in all‐solid‐state batteries when paired with argyrodite‐type Li6PS5Cl (LPSCl) solid‐state electrolyte. Our findings show that the Pyr‐IHF/LPSCl cathode delivers a high initial charge capacity of 172 mAh g−1 at a 0.1 C rate, with ca. 65 % capacity retention after 50 cycles. Advanced characterization techniques, including focused ion beam‐scanning electron microscopy, scanning electron microscopy coupled with energy dispersive X‐ray spectroscopy, and X‐ray absorption spectroscopy, indicate a pronounced redox reaction between Pyr‐IHF and LPSCl upon cell preparation, resulting in significant capacity contributions from the sulfur redox of LPSCl decomposition products during electrochemical cycling.
Genome‐Scale Community Model‐Guided Development of Bacterial Coculture for Lignocellulose Bioconversion
Authors: Kundu Pritam , Ghosh Amit 
Published: January 2025

ABSTRACTMicrobial communities have shown promising potential in degrading complex biopolymers, producing value‐added products through collaborative metabolic functionality. Hence, developing synthetic microbial consortia has become a predominant technique for various biotechnological applications. However, diverse microbial entities in a consortium can engage in distinct biochemical interactions that pose challenges in developing mutualistic communities. Therefore, a systems‐level understanding of the inter‐microbial metabolic interactions, growth compatibility, and metabolic synergisms is essential for developing effective synthetic consortia. This study demonstrated a genome‐scale community modeling approach to assess the inter‐microbial interaction pattern and screen metabolically compatible bacterial pairs for designing the lignocellulolytic coculture system. Here, we have investigated the pairwise growth and biochemical synergisms among six termite gut bacterial isolates by implementing flux‐based parameters, i.e., pairwise growth support index (PGSI) and metabolic assistance (PMA). Assessment of the PGSI and PMA helps screen nine beneficial bacterial pairs that were validated by designing a coculture experiment with lignocellulosic substrates. For the cocultured bacterial pairs, the experimentally measured enzymatic synergisms (DES) showed good coherence with model‐derived biochemical compatibility (PMA), which explains the fidelity of the in silico predictions. The highest degree of enzymatic synergisms has been observed in C. denverensis P3 and Brevibacterium sp P5 coculture, where the total cellulase activity has been increased by 53%. Hence, the flux‐based assessment of inter‐microbial interactions and metabolic compatibility helps select the best bacterial coculture system with enhanced lignocellulolytic functionality. The flux‐based parameters (PGSI and PMA) in the proposed community modeling strategy will help optimize the composition of microbial consortia for developing synthetic microcosms for bioremediation, bioengineering, and biomedical applications.
Discovery of novel benzosultam CRBN ligands
Authors: Park Hoyeong , Raikar Santosh Shivanand , Kim Yonghyo , Chae Chong Hak , Cho Yong‐Hee , Kim Pilho 
Published: January 2025

AbstractTargeted protein degradation (TPD) is a relatively novel drug discovery strategy that could help break through the limitations of traditional small molecule inhibitors. While TPD mostly utilizes diverse E3 ligases to incorporate the ubiquitin‐proteasome system (UPS), cereblon (CRBN) could be considered one of the most successfully adopted E3 ligases. Thus, expanding the scope of CRBN ligands has received tremendous attention to overcome related issues, such as selectivity and druggability. In this study, design and synthesis of novel benzosultam‐based CRBN ligands have been explored by replacement of lactam in lenalidomide with sultam. The sultam‐based ligands showed CRBN binding affinities 2‐20 times stronger than lenalidomide, presumably from additional hydrogen bonds generated from the extra oxygen atom in the sultam group, as supported by docking studies. This research highlights the potential of novel benzosultam CRBN ligands as a new tool for CRBN‐mediated TPD strategies.
Ultrasound‐guided interstitial photothermal therapy generates improved treatment responses in a 9464D model of neuroblastoma
Authors: Olsson Grace E. , Patil Rohan V. , Chin Samantha J. , Rus Katharine N. , Sweeney Elizabeth E. , Sharma Karun V. , Fernandes Rohan 
Published: January 2025

AbstractWe describe the use of ultrasound image guidance to improve treatment outcomes when administering interstitial photothermal therapy (I‐PTT), an experimental cancer treatment modality. I‐PTT is a promising thermal therapy for tumors using intratumorally injected nanoparticle‐based photothermal agents activated by an interstitially placed laser diffuser. We hypothesized that ultrasound‐based image guidance yields improved tumor treatment outcomes in terms of tumor regression and survival by improving the accuracy of the placement of the laser fiber and nanoparticles within a tumor and facilitating more precise PTT delivery. To test this hypothesis, we assessed the effect of ultrasound‐guided I‐PTT (US I‐PTT) on neuroblastoma, an aggressive solid tumor of childhood, using the 9464D syngeneic model in C57BL/6 mice. US I‐PTT using Prussian blue nanoparticles activated by an interstitial cylindrical laser diffuser generated an equivalent in vivo thermal dose as blinded, non‐image‐guided I‐PTT (B I‐PTT). However, US I‐PTT resulted in significantly higher treatment accuracy compared to B I‐PTT, attributable to the image guidance. Importantly, this improved accuracy translated to improved treatment outcomes wherein mice treated with US I‐PTT exhibited significantly improved tumor regression, tumor‐free survival, and long‐term survival compared to mice treated with B I‐PTT. Further, histological analyses of the tumors post‐PTT confirmed the advantages conferred by US I‐PTT over B I‐PTT for tumor control. These proof‐of‐concept results demonstrate the value of using ultrasound guidance for I‐PTT treatment and the translational implications of this approach to provide a more accurate and effective treatment for neuroblastoma.
Ethical Use of Cadaveric Images in Anatomical Textbooks, Atlases, and Journals: A Consensus Response From Authors and Editors
Authors: Iwanaga Joe , Kim Hee‐Jin , Akita Keiichi , Logan Bari M. , Hutchings Ralph T. , Ottone Nicolás , Nonaka Yoichi , Anand Mahindra , Burns Danny , Singh Vishram , Peris‐Celda Maria , Martinez‐Soriano Francisco , Apaydin Nihal , Hanna Amgad , Yoshioka Nobutaka , Fernandez‐Miranda Juan , Hur Mi‐Sun , Shoja Mohammadali M. , Saremi Farhood , Reina Francisco , Tabira Yoko , Carrera Anna , Spratt Jonathan D. , Ho S. Yen , Mori Shumpei , Komune Noritaka , Watanabe Koichi , Prats‐Galino Alberto , De Andrés Jose , Reina Miguel Angel , Abrahams Peter H. , Anderson Robert H. , Ibaragi Soichiro , Loukas Marios , Tubbs R. Shane 
Published: January 2025

ABSTRACTNowadays, consent to use donor bodies for medical education and research is obtained from the body donors and their families before the donation. Recently, the International Federation of Associations of Anatomists (IFAA) published guidelines that could restrict the appearance of cadaveric images in commercial anatomical resources such as textbooks and other educational products. These guidelines state that the donor must expressly consent to using such images for this purpose. Cadaveric photos and drawings made from dissections of cadavers have been used in anatomy textbooks and atlases for hundreds of years. They are invaluable for anatomy students and clinical/surgical practitioners. The IFAA guidelines should not restrict the use of those older books; to do so would infringe the rights of those seeking knowledge from these resources. As the images in such textbooks and atlases are anonymized and are used for teaching and research, and the donors and their families are informed about this before the donation, we believe no additional consent is needed. It is impossible to separate educational from “commercial” usage entirely in any situation, e.g., publications from publishers and the use of cadavers in medical schools. Therefore, our best efforts to avoid unethical use of cadaveric images by following traditional consent processes are still needed so that more people will reap the benefits from them. As senior textbook/atlas authors/editors from over 10 countries, we believe that using cadaveric images in anatomy textbooks is appropriate, and no additional consent should be necessary. Such usage falls within the good faith of professionals using these invaluable gifts.
Randomized, Placebo‐Controlled Pilot Study of Naproxen During Dental Implant Osseointegration
Authors: Kumchai Hattanas , Taub Daniel I. , Tomlinson Ryan E. 
Published: January 2025

ABSTRACTObjectivesNonsteroidal anti‐inflammatory drugs (NSAIDs) are often prescribed following the placement of dental implants, but the effects of these drugs on the osseointegration process are poorly understood. We designed a randomized, placebo‐controlled pilot study to quantitatively assess the effect of NSAIDs during early implant osseointegration.Materials and MethodsSubjects receiving a maxillary dental implant were randomized to take naproxen or placebo for 7 days after the surgery. Implant osseointegration was quantified using Resonance Frequency Analysis device. Implant‐Stability‐Quotient (ISQ) measurement was performed at the time of surgery and at follow‐up visits 1, 4, and 16 weeks after surgery. Periapical radiographs were taken to measure the marginal bone level. Separately, a questionnaire of NSAIDs usage was provided to subjects presenting with early implant failure.ResultsAfter 4 weeks, ISQ values increased modestly ( + 1%) in subjects receiving naproxen whereas subjects receiving placebo had a much larger increase in ISQ value (+41%). We observed 55% more marginal bone loss at 4 weeks, and 52% at 16 weeks in the naproxen group compared to the placebo group. These results were not found to have statistically significant between groups (p ≥ 0.05). These effect sizes and variance were used to conduct a power analysis to determine the necessary sample size for future studies. Furthermore, our separate questionnaire study revealed that 68% of our patients with early failed dental implants reported a history of NSAIDs usage after the surgery.ConclusionIn conclusion, this pilot study provides effect sizes and sample size estimates for future studies to definitively determine recommendations regarding NSAID usage following dental implant surgery. Nonetheless, our study did not observe any statistically significant differences in ISQ value or marginal bone loss after up to 16 weeks of follow‐up between subjects from naproxen and placebo groups.
QTL mapping of stem rust resistance in a Bill Brown/Gage winter wheat population
Authors: Kumssa Tadele T. , Baenziger P. S. , Rouse M. N. , Hussain Waseem , Belamkar Vikas , Wegulo Stephen N. , Poland Jesse 
Published: January 2025

AbstractThe wheat (Triticum spp.) stem rust pathogen, Puccinia graminis f. sp. tritici Eriks. and E. Henn. (Pgt), has continued to be a devastating biotic stress in wheat production. Over previous decades, scientists have identified several resistance genes effective against Pgt. However, the ever‐evolving Pgt and low availability of durable resistance necessitates continuous identification and wise deployment of resistance genes. To elucidate the identity of our previously reported stem rust resistance in hard red winter wheat cultivar Gage, we used recombinant inbred lines (RILs) developed from the cross of Bill Brown × Gage and evaluated them for 3 years for response to six different stem rust pathogen races individually at the seedling stage in the greenhouse and a mixture of these races in the field. Using molecular markers, we determined the genomic regions that affect stem rust resistance in Gage, which identified two quantitative trait loci (QTLs) at the seedling stage and one major QTL at the adult stage, giving insight into why Gage has superior stem rust resistance. The seedling stem rust resistance was from SrTmp and likely from an Sr7 allele. QTLs conferring adult plant resistance in Gage were mainly from Sr2, but molecular analysis suggested additional minor‐effect QTLs were involved.
Arginine as a Multifunctional Additive for High Performance S‐Cathode
Authors: Ren Lulu , Guo Ying , Ying Chunhua , Tangxin Zhong Justin , Liu Jin , Katie Zhong Wei‐Hong 
Published: January 2025

AbstractAdvancement of sulfur (S) cathode of lithium–sulfur (Li–S) batteries is hindered by issues such as insulating nature of sulfur, sluggish redox kinetics, polysulfide dissolution and shuttling. To address these issues, we initiate a study on applying an important amino acid of protein, arginine (Arg), as a functional additive into S cathode. Based on our simulation study, the positively charged Arg facilitates strong interactions with polysulfides. The experimental results indicate that the interaction enable capability of trapping polysulfides within the S cathode, responsible for reducing shuttle effects. Furthermore, the positively charged Arg also promotes efficient ion diffusion and polysulfides conversion. The new findings include that, with addition of only 1 wt % Arg, the resultant cathode demonstrates effectively enhanced electrolyte wettability, polysulfide adsorption and redox kinetics, leading to enhanced rate performance and long‐term cycling stability. This study highlights the great potential of amino acids being able to act as effective functional bio‐additives in S cathode, paving a new way to high‐performance and sustainable energy storage solutions.
Enhancing Stability and Activity of Fe‐Based Catalysts for Propane Dehydrogenation via Anchoring Isolated Fe‐Cl Sites
Authors: Xue Fan , Wang Jingnan , Li Panpan , Yao Yongbin , Li Junmeng , Lu Zongjing , Yi Ding , Yuan Fangli , Yan Wensheng , Wang Xi 
Published: January 2025

AbstractThe eco‐friendly features and desirable catalytic activities of Fe‐based catalysts make them highly promising for propane dehydrogenation (PDH). However, simultaneously improving their stability and activity remains a challenge. Here, we present a strategy to address these issues synergistically by anchoring single‐atom Fe−Cl sites in Al3+ vacancies of Al2O3. The as‐synthesized Fe−Cl/Al2O3 catalyst exhibited greater charge transfer between Cl and Fe than that between O and Fe in conventionally impregnated single‐atom Fe/Al2O3 catalysts, resulting in higher effective magnetic moments for Fe−Cl/Al2O3 compared to Fe/Al2O3. When tested in PDH, the durability of Fe−Cl/Al2O3 exceptionally lasted for 250 h under continuous regeneration conditions comprising 60 % C3H8 (40 % N2), followed by pure C3H8 at 600 °C while maintaining a high propylene space‐time yield of 1.2 molC3H6 gFe−1 h−1, surpassing the performance of previously developed Fe‐based PDH catalysts. We demonstrate that anchoring Fe−Cl into Al3+ vacancies simultaneously enhances stability and suppresses coke formation, owing to unique atomically dispersed Fe−Cl active structures. Compared with Fe/Al2O3 catalysts, charge transfer between Cl and Fe active centers reduces the activation energy barrier for C−H activation during C3H8 dehydrogenation, thereby improving catalytic activity; this may be related to their spin state as observed in in‐situ X‐ray emission spectroscopy studies during PDH.