bluuebunny/crossref_metadata_2025_split huggingface dataset:
The vectoring competence of the mite Varroa destructor for Deformed wing virus of honey bees is highly dynamic and affects survival of the mite
Authors: Ryabov Eugene V. , Posada-Florez Francisco , Rogers Curtis , Lamas Zachary S. , Evans Jay D. , Chen Yanping , Cook Steven C. 
Published: May 2022

AbstractThe ectoparasitic mite, Varroa destructor and the viruses it vectors, including types A and B of Deformed wing virus (DWV), pose a major threat to honey bees, Apis mellifera. Analysis of 256 mites collected from the same set of colonies on five occasions from May to October 2021 showed that less than a half of them, 39.8% (95% confidence interval (CI): 34.0 - 46.0%), were able to induce an overt-level DWV infection with more than 109 viral genomes per bee in the pupa after 6 days of feeding, with both DWV-A and DWV-B being vectored at similar rates. To investigate the effect of the phoretic stage on the mites’ ability to vector DWV, the mites from two field collection events were divided into two groups, one of which was tested immediately for their infectiveness, and the other was kept with adult worker bees in cages for 12 days prior to testing their infectiveness. We found that while 39.2 % (95% CI: 30.0 – 49.1%) of the immediately tested mites induced overt-level DWV infections, 12-day phoretic passage significantly increased the infectiousness of mites to 89.8% (95% CI: 79.2 – 95.6%). It is likely that Varroa mites that survive brood interruptions in field colonies are increasingly infectious. We found that mite lifespan was significantly affected by the type of DWV it transmitted to pupae. The mites, which induced overt DWV-B but not DWV-A infection had an average lifespan of 15.5 days (95% CI: 11.8 - 19.2 days), which was significantly shorter than those of the mites which induced overt DWV-A but not DWV-B infection, with an average lifespan of 24.3 days (95% CI: 20.2 - 28.5), or the mites which did not induce high levels of DWV-A or DWV-B, with an average survival of 21.2 days (95% CI: 19.0 - 23.5 days). The mites which transmitted high levels of both DWV-A and DWV-B had an intermediate average survival of 20.5 days (95% CI: 15.1 - 25.9 days). The negative impact of DWV-B on mite survival could be a consequence of the ability of DWV-B, but not DWV-A to replicate in Varroa mites.
Spatio-temporal diversity of dietary preferences and stress sensibilities of early and middle Miocene Rhinocerotidae from Eurasia: Impact of climate changes
Authors: Hullot Manon , Merceron Gildas , Antoine Pierre-Olivier 
Published: May 2022

AbstractMajor climatic and ecological changes are documented in terrestrial ecosystems during the Miocene epoch. The Rhinocerotidae are a very interesting clade to investigate the impact of these changes on ecology, as they are abundant and diverse in the fossil record throughout the Miocene. Here, we explored the spatio-temporal evolution of rhinocerotids’ paleoecology during the early and middle Miocene of Europe and Pakistan. We studied the dental texture microwear (proxy for diet) and enamel hypoplasia (stress indicator) of 19 species belonging to four sub-tribes and an unnamed clade of Rhinocerotidae, and coming from nine Eurasian localities ranging from Mammal Neogene zone (MN) 2 to MN7/8. Our results suggest clear differences in the feeding ecology and thus niche partitioning at Kumbi 4 (MN2, Pakistan), Sansan (MN6, France), and Villefranche d’Astarac (MN7/8, France), while overlap of the interpreted diets and subtle variations are discussed for Béon 1 (MN4, France) and Gračanica (MN5/6, Bosnia-Herzegovina). All rhinocerotids studied were interpreted as browsers or mixed-feeders, and none had a grazer nor frugivore diet. The prevalence of hypoplasia was moderate (∼10%) to high (> 20%) at all localities but Kumbi 4 (∼6%), and documented quite well the local conditions. For instance, the high prevalence at the close to Miocene Climatic Optimum locality of Béon 1 (∼26%) has been correlated with periodical droughts, while the moderate ones (∼10%) at Sansan and Devínska Nová Ves Spalte (Slovakia) both dated from the MN6 (i.e., by the middle Miocene Climatic Transition, ca. 13.9 Mya) were linked to the persistence of sub-tropical local conditions. Besides locality, species and tooth locus were also important factors of variation for the prevalence of hypoplasia. The very large hippo-likeBrachypotherium brachypuswas one of the most affected species at all concerned localities (but Sansan), whereas early-diverging elasmotheriines were very little affected, suggesting an influence of phylogeny and/or diet in stress susceptibility.
Subthalamic beta bursts correlate with dopamine-dependent motor symptoms in 106 Parkinson’s patients
Authors: Lofredi Roxanne , Okudzhava Liana , Irmen Friederike , Brücke Christof , Huebl Julius , Krauss Joachim K. , Schneider Gerd-Helge , Faust Katharina , Neumann Wolf-Julian , Kühn Andrea A. 
Published: May 2022

AbstractPathologically increased beta power has been described as a biomarker for Parkinson’s disease (PD) and related to prolonged bursts of subthalamic beta synchronization. Here, we investigate the association between subthalamic beta dynamics and motor impairment in a cohort of 106 Parkinson’s patients in the ON- and OFF-medication state, suing two different methods of beta burst determination. We report a frequency-specific correlation of low beta power and burst duration with motor impairment OFF dopaminergic medication. Furthermore, reduction of power and burst duration correlated significantly with symptom alleviation through dopaminergic medication. Importantly, qualitatively similar results were yielded with two different methods of beta burst definition. Our findings validate the robustness of previous results on pathological changes in subcortical oscillations both in the frequency-as well as in the time-domain in the largest cohort of PD patients to date with important implications for next-generation adaptive deep brain stimulation control algorithms.
A novel mouse model of postpartum depression and the neurobiological effects of fast-acting antidepressant treatments
Authors: García-Baos Alba , Ferreres-Álvarez Irene , Gallego-Landin Inés , Puig-Reyné Xavier , Castro-Zavala Adriana , Valverde Olga , Martín-Sánchez Ana 
Published: May 2022

Abstractpostpartum depression (PPD) is a severe psychiatric disorder that affects up to 15% of mothers and impairs mother-infant bonding with devastating consequences on the child development and the mother health. Several studies indicate a possible dysregulation of glutamatergic and GABAergic signalling in the corticolimbic system, as well as a downregulation of the allopregnanolone levels in serum of PPD patients. Although brexanolone, an allopregnanolone-based treatment, has recently emerged as fundamental PPD treatment, there is scarce evidence on its neurobiological action mechanism. Moreover, ketamine appears to be a promising antidepressant treatment preventing PPD, nevertheless whether it might be a more effective than allopregnanolone for some patients remain unknown. Therefore, the present study is aimed to evaluate the depressive-like phenotype of postpartum females undergoing maternal separation with early weaning (MSEW) protocol, as well as to compare the effectiveness of ketamine and allopregnanolone treatments. MSEW dams show increased despair-like behaviour, anhedonia and disrupted maternal behaviour. Moreover, lower allopregnanolone serum levels, reduction of vesicular transporters for GABA (VGAT) and glutamate (VGLUT1) in the infralimbic cortex, as well as decreased hippocampal cellular proliferation are found in MSEW females. As for the antidepressant treatments, both drugs prevent despair-like behaviour, whereas only ketamine reverts anhedonia present in MSEW females. In addition, both treatments induce pro-neurogenic effects in the dorsal hippocampus but only allopregnanolone increases the VGAT and VGLUT1, without altering the excitatory/inhibitory ratio. Altogether, we propose a new mice model that recapitulates the core symptomatology and alterations in glutamatergic and GABAergic systems shown in PPD patients, which allows us to investigate the therapeutic mechanisms of allopregnanolone and ketamine.
Orai3 and Orai1 are essential for CRAC channel function and metabolic reprogramming in B cells
Authors: Emrich Scott M. , Yoast Ryan E. , Zhang Xuexin , Fike Adam J. , Wang Yin-Hu , Bricker Kristen N. , Tao Anthony , Xin Ping , Walter Vonn , Johnson Martin T. , Pathak Trayambak , Straub Adam C. , Feske Stefan , Rahman Ziaur S.M. , Trebak Mohamed 
Published: May 2022

AbstractThe essential role of store-operated Ca2+ entry (SOCE) through Ca2+ release-activated Ca2+ (CRAC) channels in T cells is well established. In contrast, the contribution of individual Orai isoforms to SOCE and their downstream signaling functions in B cells are poorly understood. Here, we demonstrate changes in expression of Orai isoforms in response to B cell activation. We show that Orai3 and Orai1 are essential components of native CRAC channels in B cells and are critical for primary B cell proliferation and survival. The combined loss of Orai1 and Orai3 strongly impairs SOCE, nuclear factor for activated T cells (NFAT) activation, mitochondrial respiration, glycolysis, and the metabolic reprogramming of B cells in response to antigenic stimulation. Our results clarify the molecular composition and cellular functions of SOCE in B lymphocytes.
Barley powdery mildew effector CSEP0162 targets multivesicular body-associated MON1 important for immunity
Authors: Liao Wenlin , Nielsen Mads E. , Pedersen Carsten , Xie Wenjun , Thordal-Christensen Hans 
Published: May 2022

AbstractEncasements formed around haustoria and biotrophic hyphae as well as hypersensitive reaction (HR) cell death are essential plant immune responses to filamentous pathogens. Here we study a possible reason why these responses are absent in susceptible barley attacked by the powdery mildew fungus. We find that the effector CSEP0162 from this pathogen targets plant MON1, important for fusion of multivesicular bodies to their target membranes. Over-expression of CSEP0162 and silencing of barley MON1 both inhibit encasement formation. We find that the Arabidopsis ecotype No-0 has partial resistance to powdery mildew, and that this is dependent on MON1. Surprisingly, we find the MON1-dependent resistance in No-0 not only include an effective encasement response, but also HR. Similarly, silencing of MON1 in barley also blocked Mla3-mediated HR-based powdery mildew resistance. These data indicate that MON1 is a vital plant immunity component, and we speculate that the barley powdery mildew fungus introduces the effector CSEP0162 to target MON1 and reduce encasement formation and HR.HighlightMON1 is essential for MVB fusion to plasma membrane. We find that MON1 also is important for immunity, and that it is targeted by the barley powdery mildew effector CSEP0162.
A glutamine-based single ɑ-helix scaffold to target globular proteins
Authors: Escobedo A. , Piccirillo J. , Aranda J. , Diercks T. , Topal B. , Biesaga M. , Staby L. , Kragelund B. B. , García J. , Millet O. , Orozco M. , Coles M. , Crehuet R. , Salvatella X. 
Published: May 2022

AbstractThe binding of intrinsically disordered proteins to globular ones often requires the folding of motifs into ɑ-helices. These interactions offer opportunities for therapeutic intervention but their modulation with small molecules is challenging because they bury large surfaces. Linear peptides that display the residues that are key for binding can be targeted to globular proteins when they form stable helices, which in most cases requires their chemical modification. Here we present rules to design peptides that fold into single ɑ-helices by instead concatenating glutamine side chain to main chain hydrogen bonds recently discovered in polyglutamine helices. The resulting peptides are uncharged, contain only natural amino acids, and their sequences can be optimized to interact with specific targets. Our results provide design rules to obtain single ɑ-helices for a wide range of applications in protein engineering and drug design.
Email classification analysis using machine learning techniques
Authors: Iqbal Khalid , Khan Muhammad Shehrayar 
Published: May 2022

PurposeIn this digital era, email is the most pervasive form of communication between people. Many users become a victim of spam emails and their data have been exposed.Design/methodology/approachResearchers contribute to solving this problem by a focus on advanced machine learning algorithms and improved models for detecting spam emails but there is still a gap in features. To achieve good results, features also play an important role. To evaluate the performance of applied classifiers, 10-fold cross-validation is used.FindingsThe results approve that the spam emails are correctly classified with the accuracy of 98.00% for the Support Vector Machine and 98.06% for the Artificial Neural Network as compared to other applied machine learning classifiers.Originality/valueIn this paper, Point-Biserial correlation is applied to each feature concerning the class label of the University of California Irvine (UCI) spambase email dataset to select the best features. Extensive experiments are conducted on selected features by training the different classifiers.
Workplace spirituality and innovative work behavior: the role of employee flourishing and workplace satisfaction
Authors: Hunsaker William D. , Ding Wenjing 
Published: May 2022

PurposeThe purpose of this study is to explore the role of employee flourishing as a mechanism to explain the relationship between workplace spirituality and employees' innovative work behavior (IWB). Furthermore, this study investigates how the relationship between workplace spirituality and innovative behavior is moderated by employees' perceived workplace satisfaction.Design/methodology/approachBased on structural equation modeling and hierarchical regression analysis, we conducted a cross-sectional survey of 236 millennial workers in China's service and manufacturing industries.FindingsThe research findings confirmed that workplace spirituality positively predicted the innovative behavior of employees; furthermore, employee flourishing and workplace satisfaction mediated and moderated the relationship between workplace spirituality and employee innovation, respectively.Practical implicationsThis study's findings suggest that workplace spirituality unlocks employees' innovative behavior through a heightened sense of flourishing and enhanced sense of workplace satisfaction. Organizations are advised to foster a climate conducive of workplace spirituality by developing mutually aligned values. Moreover, organizations are advised to train leaders on workplace spirituality dimensions and foster workplace practices that facilitate self-reflection, job crafting and team building, as a means of broadening employees' emotional states and workplace satisfaction.Originality/valueFew studies have examined the mechanisms that shape employees' innovative behavior through workplace spirituality. This study fills several research gaps by extending the theoretical implications of workplace spirituality and employee flourishing, as demonstrated by the multi-faceted role these variables play in motivating employees' innovative behavior among Chinese millennials. Additionally, this study demonstrates that higher levels of workplace satisfaction contribute to higher levels of innovative behavior.
Bibliometric analysis on wellness tourism – citation and co-citation analysis
Authors: Suban Syed Ahamed 
Published: May 2022

PurposeTourism on wellness is a rapidly expanding segment of the travel industry; nevertheless, it is still in its infancy, and more study research is needed to develop a scientific foundation for health and wellness tourism. The study uses bibliometric indicators like as citations to determine the field structure on wellness tourism from 1998 to 2021, and the VOSviewer software to map the significant trends in wellness tourism (WT) area, to examine the present situation.Design/methodology/approachTo reach this objective, the theme of “wellness tourism” was searched in the “Scopus” database, and bibliometrics data on the publications were obtained. In total, 414 papers were found during the initial search, which was then narrowed according to the criteria. Using this strategy, the author discovered 386 records, and after removing 4 duplicates and 1 irrelevant document, the refining produced 381 related documents. The most-cited papers, significant authors, co-citation of references, sources and authors were all investigated for the publications related to WT.FindingsAccording to this report, research into wellness tourism has increased in recent years. The authors discovered two papers with over 238 “Scopus” citations and a total of 10 studies with 1414 citations. According to Scopus, the document Napieret al.receives 36.5% of citations each year. There were a total of 804 authors who published about WT between 1998 and 2021, with Smith, M, Voigt, c, and Puczkó, L being the most-cited reference authors in the subject. Han h. has the highest index of 56 of all the authors.Research limitations/implicationsThe “Scopus” database was used for bibliometric analysis, although the VOSviewer was used exclusively. This considered as a first study to utilize a bibliometric method to address this research gap, identifying the tools, journals, and, most crucially, conceptual subdomains like spa, yoga, therapy, spirituality and trekking that will be significant in future research.Originality/valueUsing a bibliometric analytic approach, this article looked at papers on wellness tourism published between 1998 and 2021. Thus, its goal is to learn more about wellness tourism and to enlighten wellness tourism scholars on the field's structure.
Website stage model for Brazilian wineries: an analysis of presence in digital and mobile media
Authors: Kieling Ana Paula , Tezza Rafael , Vargas Guilherme Lima 
Published: May 2022

Purpose
This study aims to expand previous work on website stage models by proposing a new model including a dimension encompassing digital integration and the context of mobile, digital marketing and new technologies. Also, this study aims to classify Brazilian wineries using the stage model proposed and verify if the designated stages influence the wineries’ presence in digital and mobile media.

Design/methodology/approach
Observational research and quantitative content analysis were adopted to examine a sample of winery websites (N = 150) located in different states and regions of Brazil and create a new website stage model. After the new model’s development, the authors conducted a statistic analysis by running a one-factor analysis of variance to assess the influence of the company’s stage in the use of digital media and mobile strategies, as well as its impacts on market development.

Findings
The results suggested a quite mature market regarding website stage position, even though there is still room for growth and technological innovation. Also, the research findings show that there is a positive association between the company’s stage in the use of digital and mobile media strategies. In other words, the more advanced the winery stage, the greater its digital media and mobile presence.

Originality/value
While previous studies attempted to identify wineries’ stages through models, this study presents a new approach by updating the past studies’ stages as well as providing and testing a digital media and mobile dimension. This research contributes to consolidating the Brazilian wine industry position regarding its presence in digital and mobile media, bringing reflections to organizations, society and theory development.
Modelling the group dynamics in the wine industry
Authors: Penagos-Londoño Gabriel , Ruiz-Moreno Felipe , Sellers-Rubio Ricardo 
Published: May 2022

Purpose
One of the main difficulties for wine managers is understanding and interpreting how some strategies and company behaviours could affect firms’ performance. This study aims to contribute to improve these concerns by examining the evolution of the competitive wine industry structure over time using the strategic group membership dynamics approach.

Design/methodology/approach
This study empirically analyses a data set spanning the period 2004–2014 to identify the strategic groups in the Spanish wine industry and to model their evolution over time. A time inhomogeneous hidden Markov model (HMM) is used for this purpose.

Findings
Three strategic groups are identified: Young Makers, Quality Lovers and Major Players. Young Makers are small wineries that produce low-quality wines. They are not part of a collective brand – Protected Designation of Origin – and do not invest in marketing campaigns. Quality Lovers produce the highest quality wines but offer a narrow assortment. They invest modestly in advertising, and most of them belong to a Protected Designation of Origin. Major Players produce medium-quality wines, offer a wide assortment and invest heavily in advertising. The groups seem stable over time.

Practical implications
The results show that strategic group analysis can be used to identify and compare patterns of strategic activity within the wine industry, providing a better understanding of the competitive environment.

Originality/value
No previous studies have analysed the competitive structure of the Spanish wine industry. This study delineates the structure of this industry using strategic groups, which is supported by a valid econometric model. Therefore, from a theory base perspective, this study adds new evidence to the stream of research on strategic groups by investigating their evolution over time in the wine industry and the effect of strategic group membership on performance.
Building experience and retention: the influence of principal tenure on teacher retention rates
Authors: Guthery Sarah , Bailes Lauren P. 
Published: May 2022

PurposeHiring teachers is among principals' most critical work but what remains uncertain is the relationship between a principal's tenure in a school and the rate at which they hire teachers who will stay. Teacher retention and principal experience are key predictors of school stability. This study therefore investigates the influence of principal tenure on the retention rates of teachers they hire over time.Design/methodology/approachThe authors followed 11,717 Texas principals from 1999 to 2017, and tracked the teachers they hired in each year of their tenure in a school to see if principals became more effective at hiring teachers who stay over time. The authors use regression with fixed effects and find that the longer a principal stayed in a school, the more effective they were at hiring teachers who stay to both three- and five-year benchmarks.FindingsPrincipals hire significantly more teachers who persist after they have led their first school for five or more years; however, the average principal in Texas leaves a school after four years thus never realizing those gains. The authors' second main finding indicates that principals who enter an unstable school (less than 69% retention in the two years prior to the principal's arrival) and stay at least five consecutive years, can counteract prior instability.Originality/valueThis study provides initial evidence that principals establish a great deal of building-specific situational expertise that is not easily portable or applicable in a subsequent school placement.