bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antiretroviral therapy initiation and retention among clients who received peer-delivered linkage case management and standard linkage services, Eswatini, 2016–2020: retrospective comparative cohort study
Authors: MacKellar Duncan , Hlophe Thabo , Ujamaa Dawud , Pals Sherri , Dlamini Makhosazana , Dube Lenhle , Suraratdecha Chutima , Williams Daniel , Byrd Johnita , Tobias James , Mndzebele Phumzile , Behel Stephanie , Pathmanathan Ishani , Mazibuko Sikhathele , Tilahun Endale , Ryan Caroline 
Published: March 2022

Abstract
Background
Persons living with HIV infection (PLHIV) who are diagnosed in community settings in sub-Saharan Africa are particularly vulnerable to barriers to care that prevent or delay many from obtaining antiretroviral therapy (ART).

Methods
We conducted a retrospective cohort study to assess if a package of peer-delivered linkage case management and treatment navigation services (CommLink) was more effective than peer-delivered counseling, referral, and telephone follow-up (standard linkage services, SLS) in initiating and retaining PLHIV on ART after diagnosis in community settings in Eswatini. HIV-test records of 773 CommLink and 769 SLS clients aged ≥ 15 years diagnosed between March 2016 and March 2018, matched by urban and rural settings of diagnosis, were selected for the study. CommLink counselors recorded resolved and unresolved barriers to care (e.g., perceived wellbeing, fear of partner response, stigmatization) during a median of 52 days (interquartile range: 35—69) of case management.

Results
Twice as many CommLink than SLS clients initiated ART by 90 days of diagnosis overall (88.4% vs. 37.9%, adjusted relative risk (aRR): 2.33, 95% confidence interval (CI): 1.97, 2.77) and during test and treat when all PLHIV were eligible for ART (96.2% vs. 37.1%, aRR: 2.59, 95% CI: 2.20, 3.04). By 18 months of diagnosis, 54% more CommLink than SLS clients were initiated and retained on ART (76.3% vs. 49.5%, aRR: 1.54, 95% CI: 1.33, 1.79). Peer counselors helped resolve 896 (65%) of 1372 identified barriers of CommLink clients. Compared with clients with ≥ 3 unresolved barriers to care, 42% (aRR: 1.42, 95% CI: 1.19, 1.68) more clients with 1–2 unresolved barriers, 44% (aRR: 1.44, 95% CI: 1.25, 1.66) more clients with all barriers resolved, and 54% (aRR: 1.54, 95% CI: 1.30, 1.81) more clients who had no identified barriers were initiated and retained on ART by 18 months of diagnosis.

Conclusions
To improve early ART initiation and retention among PLHIV diagnosed in community settings, HIV prevention programs should consider providing a package of peer-delivered linkage case management and treatment navigation services. Clients with multiple unresolved barriers to care measured as part of that package should be triaged for differentiated linkage and retention services.
Agrobacterium species bacteraemia, Switzerland, 2008 to 2019: a molecular epidemiological study
Authors: Balmer Lisa , Seth-Smith Helena M. B. , Egli Adrian , Casanova Carlo , Kronenberg Andreas , Schrenzel Jacques , Marschall Jonas , Sommerstein Rami 
Published: March 2022

Abstract
Background
Agrobacterium spp. are infrequent agents of bloodstream infections linked to healthcare-associated outbreaks. However, it is unclear if outbreaks also occur across larger geographic areas. Triggered by two local clusters from putative point sources, our aim was to detect potential additional clusters in Switzerland.

Methods
We performed a nationwide descriptive study of cases in Switzerland based on a prospective surveillance system (Swiss Centre for Antibiotic Resistance, anresis.ch), from 2008 to 2019. We identified patients with Agrobacterium spp. isolated from blood cultures and used a survey to collect clinical-epidemiological information and susceptibility testing results. We performed whole genome sequencing (WGS) of available clinical isolates and determined their relatedness by single nucleotide polymorphism (SNP) variant calling analysis.

Results
We identified a total of 36 cases of Agrobacterium spp. from blood samples over 10 years. Beyond previously known local clusters, no new ones were identified. WGS-based typing was performed on 22 available isolates and showed no clonal relationships between newly identified isolates or to those from the known clusters, with all isolates outside these clusters being at least 50 SNPs apart.

Conclusion and relevance
Agrobacterium spp. bacteraemia is infrequently detected and, given that it may be healthcare-associated and stem from a point source, occurrence of multiple episodes should entail an outbreak investigation. With the help of the national antimicrobial resistance surveillance system we identified multiple clinical cases of this rare pathogen but found no evidence by WGS that suggested a nation-wide outbreak.

Graphical abstract
Factors associated with disordered feeding among high school students in Kerman City, Iran
Authors: Dokhani Abolfazl , Dehghan Mahlagha , Rayani Masoud , Maazallahi Mahboobeh , Forouzi Mansooreh Azzizadeh 
Published: March 2022

Abstract
Background
Mental health problems and disordered eating, are more common in adolescents. This study investigated relationship between mental health and disordered eating in high school girls in southeast Iran.

Methods
This cross-sectional descriptive correlational study accomplished in high school girls of Kerman at the southeast of Iran in 2019. Using three parts demographic, Standard General Health Questionnaire (GHQ-28) and Eating Attitudes questionnaire, (Eat-26) with three subscales: eating habits, desire to eat and oral control. We investigated high school girl’s mental health and relationship with disordered eating with cluster sampling method (600 high school girl). Multivariate logistic regression was used to determine the association between significant variables and the risk of General Health (Yes/No) and Eating attitude (Yes/No). Spearman correlation test, Mann–Witheny U test and Kruskal–Wallis test were used, and Significant level was considered at P < 0.05.

Results
A direct and significant relationship between mental health and disordered eating (r = 0/19, P < 0.001). In other words, the higher the mental health disorder score, the higher the disordered eating score, and the higher disordered eating score, the higher mental problems. There was a direct and significant relationship between mental health and all aspects of disordered eating including eating habits (r = 0/12, P < 0.05), desire to eat (r = 0/1, P < 0.05) and oral control (r = 0/14, P < 0.001).

Conclusions
It seems that the disordered eating and mental health have a determinant role in relationship with each other. Therefore, prevention and health promotion programs should be implemented to improve female adolescent mental health and reduce disordered eating.
Aβ oligomers trigger necroptosis-mediated neurodegeneration via microglia activation in Alzheimer’s disease
Authors: Salvadores Natalia , Moreno-Gonzalez Ines , Gamez Nazaret , Quiroz Gabriel , Vegas-Gomez Laura , Escandón Marcela , Jimenez Sebastian , Vitorica Javier , Gutierrez Antonia , Soto Claudio , Court Felipe A. 
Published: March 2022

AbstractAlzheimer’s disease (AD) is a major adult-onset neurodegenerative condition with no available treatment. Compelling reports point amyloid-β (Aβ) as the main etiologic agent that triggers AD. Although there is extensive evidence of detrimental crosstalk between Aβ and microglia that contributes to neuroinflammation in AD, the exact mechanism leading to neuron death remains unknown. Using postmortem human AD brain tissue, we show that Aβ pathology is associated with the necroptosis effector pMLKL. Moreover, we found that the burden of Aβ oligomers (Aβo) correlates with the expression of key markers of necroptosis activation. Additionally, inhibition of necroptosis by pharmacological or genetic means, reduce neurodegeneration and memory impairment triggered by Aβo in mice. Since microglial activation is emerging as a central driver for AD pathogenesis, we then tested the contribution of microglia to the mechanism of Aβo-mediated necroptosis activation in neurons. Using an in vitro model, we show that conditioned medium from Aβo-stimulated microglia elicited necroptosis in neurons through activation of TNF-α signaling, triggering extensive neurodegeneration. Notably, necroptosis inhibition provided significant neuronal protection. Together, these findings suggest that Aβo-mediated microglia stimulation in AD contributes to necroptosis activation in neurons and neurodegeneration. As necroptosis is a druggable degenerative mechanism, our findings might have important therapeutic implications to prevent the progression of AD.
A prospective observational cohort study to identify inflammatory biomarkers for the diagnosis and prognosis of patients with sepsis
Authors: D’Onofrio Valentino , Heylen Dries , Pusparum Murih , Grondman Inge , Vanwalleghem Johan , Meersman Agnes , Cartuyvels Reinoud , Messiaen Peter , Joosten Leo A. B. , Netea Mihai G. , Valkenborg Dirk , Ertaylan Gökhan , Gyssens Inge C. 
Published: March 2022

Abstract
Background
Sepsis is a life-threatening organ dysfunction. A fast diagnosis is crucial for patient management. Proteins that are synthesized during the inflammatory response can be used as biomarkers, helping in a rapid clinical assessment or an early diagnosis of infection. The aim of this study was to identify biomarkers of inflammation for the diagnosis and prognosis of infection in patients with suspected sepsis.

Methods
In total 406 episodes were included in a prospective cohort study. Plasma was collected from all patients with suspected sepsis, for whom blood cultures were drawn, in the emergency department (ED), the department of infectious diseases, or the haemodialysis unit on the first day of a new episode. Samples were analysed using a 92-plex proteomic panel based on a proximity extension assay with oligonucleotide-labelled antibody probe pairs (OLink, Uppsala, Sweden). Supervised and unsupervised differential expression analyses and pathway enrichment analyses were performed to search for inflammatory proteins that were different between patients with viral or bacterial sepsis and between patients with worse or less severe outcome.

Results
Supervised differential expression analysis revealed 21 proteins that were significantly lower in circulation of patients with viral infections compared to patients with bacterial infections. More strongly, higher expression levels were observed for 38 proteins in patients with high SOFA scores (> 4), and for 21 proteins in patients with worse outcome. These proteins are mostly involved in pathways known to be activated early in the inflammatory response. Unsupervised, hierarchical clustering confirmed that inflammatory response was more strongly related to disease severity than to aetiology.

Conclusion
Several differentially expressed inflammatory proteins were identified that could be used as biomarkers for sepsis. These proteins are mostly related to disease severity. Within the setting of an emergency department, they could be used for outcome prediction, patient monitoring, and directing diagnostics.
Trail registration number: clinicaltrial.gov identifier NCT03841162.
Antarctic yeasts: potential use in a biologic treatment of textile azo dyes
Authors: Ruscasso F. , Cavello I. , Curutchet G. , Cavalitto S. 
Published: March 2022

AbstractWe investigated the dye-removal potential of a collection of 61 cold-adapted yeasts from the King George Island, Antarctica, on agar plates supplemented with 100 mg L–1 of several textile dyes; among which isolates 81% decolorized Reactive Black 5 (RB-5), with 56% decolorizing Reactive Orange 16, but only 26% doing so with Reactive Blue 19 and Acid Blue 74. Furthermore, we evaluated the ligninolytic potential using 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic-acid) diammonium salt-, 3,5-dimethoxy-4-hydroxybenzaldehydazine-, or manganese-supplemented plates but detected no activity, possibly due to a dye-removal mechanism involving reductases. The removal kinetics were studied in liquid medium supplemented with 100 mg L–1 of RB-5 in a selection of 9 yeasts. The highest volumetric-removal rates (η) were found for Candida sake 41E (4.14 mg L–1 h–1), Leucosporidium muscorum F20A (3.90 mg L–1 h–1), and Cystofilobasidium infirmominiatum F13E (3.90 mg L–1 h–1). Different UV–Vis spectra were obtained if the dye removal occurred by biodegradation or biosorption/bioaccumulation. L. muscorum F20A was selected to study the dye-removal mechanism of RB-5 and the effect of different chemical and environmental parameters on the process. Optimum dye-removal conditions were obtained with 10 g L–1 of glucose within an initial medium pH range of 5.0 to 6.0. Up to 700 mg L–1 of dye could be removed in 45 h. High-performance liquid chromatography profiles obtained were consistent with a biodegradation of the dye. Phytotoxicity was estimated by calculating the 50%-inhibition concentration (IC50) with Lactuca sativa L. seeds. These findings propose psychrophilic yeasts as a novel environmentally suitable alternative for the treatment of dye-industry wastewaters.
Immobilization of engineered E. coli cells for asymmetric reduction of methyl acetoacetate to methyl-(R)-3-hydroxybutyrate
Authors: Chen Qing-Sheng , Yuan Xin , Peng Fei , Lou Wen-Yong 
Published: March 2022

AbstractThe efficient asymmetric bio-synthesis of chiral β-hydroxy esters is of great importance for industrial production. In this work, a simple and productive engineered E.coli cell-immobilized strategy was applied for the asymmetric reduction of MAA to (R)-HBME with high enantioselectivity. Compared with the corresponding inactivated free cells, the alginate-immobilized cells remained 45% of initial activity at 50 ℃ and 65% after reuse of 10 times. After 60 days of storage at 4 ℃, the immobilized cells maintained more than 80% relative activity. Immobilization contributed significantly to the improvement of thermal stability, pH tolerance, storage stability and operation stability without affecting the yield of product. The immobilized recombinant E. coli cell had absolute enantioselectivity for the asymmetric reduction of MAA to (R)-HBME with e.e. > 99.9%. Therefore, microbial cell immobilization is a perspective approach in asymmetric synthesis of chiral β-hydroxy esters for industrial applications.
Graphical Abstract
Relation between lipid profile, blood pressure and retinopathy in diabetic patients in King Abdulaziz University hospital: a retrospective record review study
Authors: Alattas Khadijah , Alsulami Dania W. , Alem Rahaf H. , Alotaibi Felwa S. , Alghamdi Bayan A. , Baeesa Layan S. 
Published: March 2022

Abstract
Background
Diabetic retinopathy (DR) is a major cause of blindness worldwide, threatening the vision of approximately 10% of patients with diabetes. Many studies have demonstrated that intensive control of the risk factors for DR is essential to reduce the onset and progression of DR. Currently, the relationship between lipid profile and DR is still unclear, especially in Saudi Arabia. We aimed to assess the correlation between both the development and severity of DR with lipid profile and blood pressure among the diabetic patients at the King Abdul-Aziz University hospital in Jeddah, Saudi Arabia.

Methods
This was a retrospective record review study of 298 diabetic patients diagnosed with DR. Retinal findings were correlated to serum lipids levels using univariate, bivariate, and multivariate analysis.

Results
This study included 298 participants with DR. Triglyceride levels, systolic blood pressure, low-density lipoprotein cholesterol levels, and presence of macular edema were significantly associated with DR progression (P = 0.012, P = 0.001, P = 0.002). Other parameters, including total cholesterol, high-density lipoprotein cholesterol, HbA1C, body mass index, age, were not significantly associated with DR.

Conclusion
Elevation in serum triglyceride levels and systolic blood pressure showed a statically significant association with diabetic retinopathy. Controlling these factors may help preventing progression and occurrence of diabetic retinopathy among diabetic patients.
Intraoperative myocardial infarction and refractory cardiogenic shock during major hepatectomy: a case report
Authors: Yagi Yasunori , Mizunoya Kazuyuki , Mori Toshihiro , Saito Hitoshi , Morimoto Yuji 
Published: March 2022

Abstract
Background
Myocardial infarction (MI) complicated by cardiogenic shock during non-cardiac surgery is a rare but fatal complication. The management of intraoperative MI is challenging.

Case presentation
A 77-year-old hypertensive man with good functional capacity was scheduled for hepatectomy. After the start of liver resection, the electrocardiogram monitor showed ST depression, and the patient developed refractory cardiogenic shock. Transesophageal echocardiography revealed severe hypokinesis of the anteroseptal wall. The surgery was suspended, and an intra-aortic balloon pump was placed following immediate abdominal closure. Coronary angiography revealed severe stenosis of the left main coronary trunk, and percutaneous coronary intervention (PCI) was performed. Myocardial wall motion improved, and blood pressure stabilized. Two days after PCI, hepatectomy, which had been suspended, was successfully completed.

Conclusions
Once intraoperative MI has occurred, early diagnosis and multidisciplinary approaches are important to manage the difficult clinical situation.
It’s Not Always SIAD: Immunotherapy-Triggered Endocrinopathies Enter the Field of Cancer-Related Hyponatremia
Authors: Bischoff Jenny , Fries Charlotte , Heer Alexander , Hoffmann Friederike , Meyer Carsten , Landsberg Jennifer , Fenske Wiebke K 
Published: March 2022

Abstract
While the syndrome of inadequate antidiuresis (SIAD) is still the most common cause of hyponatremia in cancer patients, the rise in endocrine immune-related adverse events (irAEs) owing to immune checkpoint inhibitors (ICI) considerably shaped the differential diagnosis of electrolyte disorders in cancer patients. We report here 3 cases of different endocrine irAEs, first manifesting with new-onset hyponatremia under ICI therapy for malignant melanoma: one with primary adrenal insufficiency, one with hypophysitis, and one with autoimmune type 1 diabetes. Early diagnosis of endocrine toxicities can save lives but may be challenging and essentially delayed by subtle or nonspecific clinical presentation and a lack of readily available endocrinological laboratory evaluation in the primary care setting.
This exemplary case series demonstrates the broad spectrum of endocrinopathies that physicians should be aware of under ICI therapy and emphasizes new-onset hyponatremia as a possibly early, simple, and low-cost biomarker of irAEs, which may be considered as a red flag in patients receiving checkpoint blockade. As ICI-induced endocrinopathies are still under-represented in clinical practice guidelines, we here propose an updated algorithm for diagnosis of cancer-related hyponatremia, highlighting the important diagnostic steps to be considered before making the diagnosis of SIAD.
Efficacy of linking Breast Awareness Clinics in Gynecology and Obstetrics departments: A pilot project in Zubaida Bani Wing, Fazle-Omar Hospital, Chenab Nagar, Pakistan
Authors: Tahir Durr-e-Samin , Tauseef Wasima , Madiha Bareah , Urooj Tahira 
Published: March 2022

Objectives: Pakistani female population has the highest incidence of Breast cancer among the women belonging to all other Asian countries. This contributed to 28.7% of all new cases of malignancies in 2020 and is also the cause of uppermost cancer mortality in Pakistani females. The number of deaths can be reduced by promoting breast cancer screening, according to recommended programs for high-risk populations.
Methods: In order to encourage breast self-examination in women, 12 Breast Cancer Awareness Clinics were set up in Gynecology and Obstetrics outdoor, Zubaida Bani Wing, Fazle Omar Hospital, Chenab Nagar, Pakistan, from September 2019 to February 2020. A total of 238 women with a mean of 29.97±8.9 years were examined and trained to perform the breast self-examination.
Results: Around 41% of 222 valid responded women reported that they had knowledge that breast cancer is the major threat to their health in Pakistan. Only 20% were aware of breast cancer screening but poor cognizance about the recommendations for Pakistani women. Knowledge about Breast Self-Examination was recorded in 15%, whereas only 5.4% of all the women included in the present study reported to practice it. Moreover, eighty-six percent of the respondents were reported to be hesitant to visit the male doctor. Three cases of most suspected malignancies were referred to specialist consultants and few cases were endorsed for immediate mammograms.
Conclusion: Setting up breast awareness and screening clinics at gynecology outdoors with trained female assistants can be effective in promoting Breast Self-Examination and elaborating screening programs countywide to obtain long term benefits in a high-risk population of Pakistan.
doi: https://doi.org/10.12669/pjms.38.4.5440
How to cite this:Tahir DS, Tauseef W, Madiha B, Urooj T. Efficacy of linking Breast Awareness Clinics in Gynecology and Obstetrics departments: A pilot project in Zubaida Bani Wing, Fazle-Omar Hospital, Chenab Nagar, Pakistan. Pak J Med Sci. 2022;38(4):---------. doi: https://doi.org/10.12669/pjms.38.4.5440
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Innovations in peer review in scholarly publishing: a meta-summary
Authors: Woods Helen Buckley , Brumberg Johanna , Kaltenbrunner Wolfgang , Pinfield Stephen , Waltman Ludo 
Published: March 2022

Background: There are currently numerous innovations in peer review and quality assurance in scholarly publishing. The Research on Research Institute conducted a programme of co-produced projects investigating these innovations. This literature review was part of one such project ‘Experiments in peer review’ which created an inventory and framework of peer review innovations. The aim of this literature review was to aid the development of the inventory by identifying innovations in peer review reported in the scholarly literature and by providing a summary of the different approaches. Methods: This meta-summary is based on data identified from Web of Science and Scopus limited from 2010 to 2021. A total of 247 papers were screened, with 6 review articles chosen for the focus of the literature review. Items were selected that described approaches to innovating peer review or illustrated examples. Results: The summary of innovations are drawn from 6 review articles. The innovations are divided into three high-level categories: approaches to peer review, reviewer focussed initiatives and technology to support peer review with sub-categories of results presented in tabular form and summarised. A summary of all innovations found is also presented. Conclusions: From a simple synthesis of the review authors’ conclusions, three key messages are presented: observations on current practice; authors’ views on the implications of innovations in peer review; and calls for action in peer review research and practice.