bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alcohol use disorder with comorbid anxiety disorder: a case report and focused literature review
Authors: Mocanu Victor , Wood Evan 
Published: November 2022

Abstract
Background
Alcohol use disorder (AUD) and anxiety disorders (AnxD) are prevalent health concerns in clinical practice which frequently co-occur (AUD-AnxD) and compound one another. Concurrent AUD-AnxD poses a challenge for clinical management as approaches to treatment of one disorder may be ineffective or potentially counterproductive for the other disorder.

Case Presentation
We present the case of a middle-aged man with anxiety disorder, AUD, chronic pain, and gamma-hydroxybutyrate use in context of tapering prescribed benzodiazepines who experienced severe alcohol withdrawal episodes during a complicated course of repeated inpatient withdrawal management. After medical stabilization, the patient found significant improvement in symptoms and no return to alcohol use with a regimen of naltrexone targeting his AUD, gabapentin targeting both his AUD and AnxD, and engagement with integrated psychotherapy, Alcoholics Anonymous, and addictions medicine follow-up.

Conclusion
Proper recognition and interventions for AUD and AnxD, ideally with overlapping efficacy, can benefit individuals with comorbid AUD-AnxD. Gabapentin, tobacco cessation, and integrated psychotherapy have preliminary evidence of synergistic effects in AUD-AnxD. Meta-analysis evidence does not support serotoninergic medications (e.g. selective serotonin reuptake inhibitors) which are commonly prescribed in AnxD and mood disorders as their use has not been associated with improved outcomes for AUD-AnxD. Additionally, several double-blind placebo-controlled randomized trials have suggested that serotonergic medications may worsen alcohol-related outcomes in some individuals with AUD. Areas for future investigation are highlighted.
Low expression of PEBP1P2 promotes metastasis of clear cell renal cell carcinoma by post-transcriptional regulation of PEBP1 and KLF13 mRNA
Authors: Yang Lei , Yin Haoli , Chen Yi , Pan Chun , Hang Hexing , Lu Yanwen , Ma Wenliang , Li Xin , Gan Weidong , Guo Hongqian , Li Dongmei 
Published: November 2022

Abstract
Background
Pseudogenes play an essential role in tumor occurrence and progression. However, the functions and mechanisms of pseudogenes in clear cell renal cell carcinoma (ccRCC) remain largely elusive.

Methods
We quantified PEBP1P2 expression in ccRCC tissues and cells using fluorescence in situ hybridization and real-time PCR. Besides, we evaluated the role of PEBP1P2 in ccRCC using a lung metastasis model and a transwell assay. Finally, we documented the interactions between PEBP1P2, PEBP1, and KLF13 by performing luciferase, RNA immunoprecipitation, RNA pulldown, and targeted RNA demethylation assays.

Results
Low PEBP1P2 expression correlates significantly with advanced stages and poor prognosis in ccRCC patients. Besides, PEBP1P2 overexpression inhibits ccRCC metastasis formation in vivo and in vitro. Interestingly, PEBP1P2 directly interacted with 5-methylcytosine (m5C)-containing PEBP1 mRNA and recruited the YBX1/ELAVL1 complex, stabilizing PEBP1 mRNA. In addition, PEBP1P2 increased KLF13 mRNA levels by acting as a sponge for miR-296, miR-616, and miR-3194.

Conclusions
PEBP1P2 inhibits ccRCC metastasis formation and regulates both PEBP1 and KLF13. Therefore, molecular therapies targeting PEBP1P2 might be an effective treatment strategy against ccRCC and other cancers with low PEBP1P2 levels.
A review on the application of the exposome paradigm to unveil the environmental determinants of age-related diseases
Authors: Ding Enmin , Wang Yu , Liu Juan , Tang Song , Shi Xiaoming 
Published: November 2022

AbstractAge-related diseases account for almost half of all diseases among adults worldwide, and their incidence is substantially affected by the exposome, which is the sum of all exogenous and endogenous environmental exposures and the human body’s response to these exposures throughout the entire lifespan. Herein, we perform a comprehensive review of the epidemiological literature to determine the key elements of the exposome that affect the development of age-related diseases and the roles of aging hallmarks in this process. We find that most exposure assessments in previous aging studies have used a reductionist approach, whereby the effect of only a single environmental factor or a specific class of environmental factors on the development of age-related diseases has been examined. As such, there is a lack of a holistic and unbiased understanding of the effect of multiple environmental factors on the development of age-related diseases. To address this, we propose several research strategies based on an exposomic framework that could advance our understanding—in particular, from a mechanistic perspective—of how environmental factors affect the development of age-related diseases. We discuss the statistical methods and other methods that have been used in exposome-wide association studies, with a particular focus on multiomics technologies. We also address future challenges and opportunities in the realm of multidisciplinary approaches and genome–exposome epidemiology. Furthermore, we provide perspectives on precise public health services for vulnerable populations, public communications, the integration of risk exposure information, and the bench-to-bedside translation of research on age-related diseases.
Preparation of vitamin D3-loaded oil-in-water-in-oil double emulsions using psyllium gum: optimization using response surface methodology
Authors: Didar Zohreh , Hesarinejad Mohammad Ali 
Published: November 2022

AbstractIn this study, the encapsulation of vitamin D3 into a double emulsion with psyllium gum in the aqueous phase and lecithin in the oil phase was optimized and modeled. The optimal values of the three independent variables were generated using a faced-centered central composite design (FCCD). The Z-average (diameter of the emulsion droplets), polydispersity index (PDI), zeta potential, interfacial tension, creaming index, and encapsulation efficiency are among the quality evaluation metrics. According to the findings, the Z-average in the double emulsion was inversely affected by the psyllium gum concentration. The findings indicated that time after production had a significant direct influence on the Z-average. All freshly manufactured formulations may be characterized as good stable emulsions, according to the measurement of double emulsions’ zeta potential after preparation (negative charge lower than − 40.1 mV). During storage, the zeta potential value exhibited an upward trend. The creaming index was influenced significantly by storage time (p < 0.05) and at the end of storage time, the creaming index was 19.2% (in the sample with no gum and containing 0.25% lecithin). Analysis revealed that the interfacial tension was reduced as a result of the inclusion of the psyllium gum. On the other hand, prolonging storage lengthened the interfacial tension's magnitude. According to the findings, gum content and time had a significant impact on the encapsulation efficiency of primary and double emulsions. Finally, the optimal double emulsion preparation parameters based on maximum encapsulation efficiency were 1% psyllium gum, 1.125% lecithin, and a storage time equal to 25 days at 8 °C, with an obtained encapsulation efficiency of the double emulsion of 93.26%.
Derivation of 1.064 μm normal albedos on the C-type asteroid Ryugu from laser pulse intensity measurement of the Hayabusa2 LIDAR
Authors: Yamada Ryuhei , Yamamoto Keiko , Oshigami Shoko , Araki Hiroshi , Senshu Hiroki , Noda Hirotomo , Namiki Noriyuki , Matsumoto Koji , Yoshida Fumi , Abe Shinsuke , Hirata Naru , Sasaki Sho , Mizuno Takahide 
Published: November 2022

AbstractJapanese asteroid explorer Hayabusa2 arrived at C-type asteroid 162,173 Ryugu in June 2018. The laser altimeter (LIDAR) onboard Hayabusa2 measured its own transmitted laser and returned pulse intensities from a Ryugu surface until November 2019. Because the Ryugu surface is extremely rough, topography dominates over the material properties in the conventional derivation of normal albedo. Thus, we developed a method to retrieve the normal albedo from the rough surface of a C-type asteroid at a LIDAR laser wavelength of 1.064 μm. The albedo map covering an equatorial band between – 40° and + 20° in latitude was created with 3˚-by-3˚ resolution using the intensity data obtained before the conjunction of the spacecraft with the Sun. The average of the normal albedo is 0.0405 $$\pm$$
±
0.0027, whereas approximately half of the 3°-by-3° grids are between 0.04 and 0.045. The low and uniform normal albedo feature is common to other remote-sensing observations of Ryugu by visible and near-infrared cameras onboard Hayabusa2.
Graphical Abstract
Global marine gravity anomalies from multi-satellite altimeter data
Authors: Wan Xiaoyun , Hao Ruijie , Jia Yongjun , Wu Xing , Wang Yi , Feng Lei 
Published: November 2022

AbstractIn this study, China’s first altimeter satellite Haiyang-2A (HY-2A) data combined observations from CryoSat-2, SARAL/AltiKa, and Jason-1&2 are used to calculate the global (60°S–60°N) marine deflections of the vertical and gravity anomalies named Global Marine Gravity Anomaly Version 1(GMGA1), with grid resolution of 1′ × 1′. The deflections of the vertical from each satellite observations are first derived from the gradients of the geoid height through the least squares method. The deflections of the vertical are then merged by assigning different weights to each satellite product based on their accuracy. Finally, gravity anomalies are obtained by the remove-restore method. The results reveal that the fused deflections of the vertical have an accuracy of 0.4 arcsec in the north component and 0.8 arcsec in the east component. HY-2A’s contribution to the north component of the integrated deflections of the vertical is second only to Cryosat-2. Jason-1/2 accounts for a large proportion of the integrated east components. Compared to worldwide products such as DTU17, Sandwell & Smith V31.1, as well as values from EGM2008, EIGEN-6C4 and XGM2019e_2159, GMGA1 has an accuracy of around 3.3 mGal. By not using HY-2A data, the precision of GMGA1 is reduced by about 0.1 mGal. To further improve the accuracy, seafloor topography information is used to provide short wavelength gravity anomaly. It is verified in the South China Sea (112°E–119E°, 12°N–20°N) using the Parker formula. By combining shipborne depth generated data and GMGA1 through a filtering technique, a new version of gravity anomaly grid with an accuracy improvement of 0.4 mGal in the South China Sea is obtained.
Graphical Abstract
Effect of noradrenaline on propofol-induced mitochondrial dysfunction in human skeletal muscle cells
Authors: Krajčová Adéla , Skagen Christine , Džupa Valér , Urban Tomáš , Rustan Arild C. , Jiroutková Kateřina , Bakalář Bohumil , Thoresen G. Hege , Duška František 
Published: November 2022

Abstract
Background
Mitochondrial dysfunction is a hallmark of both critical illness and propofol infusion syndrome and its severity seems to be proportional to the doses of noradrenaline, which patients are receiving. We comprehensively studied the effects of noradrenaline on cellular bioenergetics and mitochondrial biology in human skeletal muscle cells with and without propofol-induced mitochondrial dysfunction.

Methods
Human skeletal muscle cells were isolated from vastus lateralis biopsies from patients undergoing elective hip replacement surgery (n = 14) or healthy volunteers (n = 4). After long-term (96 h) exposure to propofol (10 µg/mL), noradrenaline (100 µM), or both, energy metabolism was assessed by extracellular flux analysis and substrate oxidation assays using [14C] palmitic and [14C(U)] lactic acid. Mitochondrial membrane potential, morphology and reactive oxygen species production were analysed by confocal laser scanning microscopy. Mitochondrial mass was assessed both spectrophotometrically and by confocal laser scanning microscopy.

Results
Propofol moderately reduced mitochondrial mass and induced bioenergetic dysfunction, such as a reduction of maximum electron transfer chain capacity, ATP synthesis and profound inhibition of exogenous fatty acid oxidation. Noradrenaline exposure increased mitochondrial network size and turnover in both propofol treated and untreated cells as apparent from increased co-localization with lysosomes. After adjustment to mitochondrial mass, noradrenaline did not affect mitochondrial functional parameters in naïve cells, but it significantly reduced the degree of mitochondrial dysfunction induced by propofol co-exposure. The fatty acid oxidation capacity was restored almost completely by noradrenaline co-exposure, most likely due to restoration of the capacity to transfer long-chain fatty acid to mitochondria. Both propofol and noradrenaline reduced mitochondrial membrane potential and increased reactive oxygen species production, but their effects were not additive.

Conclusions
Noradrenaline prevents rather than aggravates propofol-induced impairment of mitochondrial functions in human skeletal muscle cells. Its effects on bioenergetic dysfunctions of other origins, such as sepsis, remain to be demonstrated.
Co-designing person-centred quality indicator implementation for primary care in Alberta: a consensus study
Authors: Manalili Kimberly , Scott Catherine M. , Hemmelgarn Brenda , O’Beirne Maeve , Bailey Allan L. , Haener Michel K. , Banerjee Cyrene , Peters Sue P. , Chiodo Mirella , Aghajafari Fariba , Santana Maria J. 
Published: November 2022

Abstract
Background
We aimed to contribute to developing practical guidance for implementing person-centred quality indicators (PC-QIs) for primary care in Alberta, Canada. As a first step in this process, we conducted stakeholder-guided prioritization of PC-QIs and implementation strategies. Stakeholder engagement is necessary to ensure PC-QI implementation is adapted to the context and local needs.

Methods
We used an adapted nominal group technique (NGT) consensus process. Panelists were presented with 26 PC-QIs, and implementation strategies. Both PC-QIs and strategies were identified from our extensive previous engagement of patients, caregivers, healthcare providers, and quality improvement leaders. The NGT objectives were to: 1. Prioritize PC-QIs and implementation strategies; and 2. Facilitate the participation of diverse primary care stakeholders in Alberta, including patients, healthcare providers, and quality improvement staff. Panelists participated in three rounds of activities. In the first, panelists individually ranked and commented on the PC-QIs and strategies. The summarized results were discussed in the second-round face-to-face group meeting. For the last round, panelists provided their final individual rankings, informed by the group discussion. Finally, we conducted an evaluation of the consensus process from the panelists’ perspectives.

Results
Eleven primary care providers, patient partners, and quality improvement staff from across Alberta participated. The panelists prioritized the following PC-QIs: ‘Patient and caregiver involvement in decisions about their care and treatment’; ‘Trusting relationship with healthcare provider’; ‘Health information technology to support person-centred care’; ‘Co-designing care in partnership with communities’; and ‘Overall experience’. Implementation strategies prioritized included: ‘Develop partnerships’; ‘Obtain quality improvement resources’; ‘Needs assessment (stakeholders are engaged about their needs/priorities for person-centred measurement)’; ‘Align measurement efforts’; and ‘Engage champions’. Our evaluation suggests that panelists felt that the process was valuable for planning the implementation and obtaining feedback, that their input was valued, and that most would continue to collaborate with other stakeholders to implement the PC-QIs.

Conclusions
Our study demonstrates the value of co-design and participatory approaches for engaging stakeholders in adapting PC-QI implementation for the primary care context in Alberta, Canada. Collaboration with stakeholders can promote buy-in for ongoing engagement and ensure implementation will lead to meaningful improvements that matter to patients and providers.
Acute severe asthma in emergency department: clinical characteristics, risk factors, and predictors for poor outcome
Authors: Mohamed Ashraf Zin E L-Abdeen , Shaaban Lamiaa Hassan , Gad Shereen Frghaly , Azeem Esraa Abdel , Gamal Elddin Waleed 
Published: November 2022

Abstract
Background
Severe asthma exacerbation can be a frightening experience to the patient and physician. Despite continuous efforts to frame management guidelines and advances in treatment, severe exacerbations still occur. In order to prevent and judicious management of asthma exacerbations, we should predict them first. This study aims to evaluate distinct clinical trajectories and management outcome of patients with severe asthma exacerbations and also evaluate predictors for poor outcome.

Methods
Patients suffering from acute asthma exacerbation and presented to emergency room (forty patients) were grouped into 2 groups (groups A and B) according to severity of exacerbation. Assessment included full clinical history, laboratory investigations (including eosinophil cell count and serum IgE level), Beck’s anxiety and depression inventory scales, assessment of asthma medication adherence and control level, and peak expiratory flow measurement (at presentation, 1 and 6 h after).

Results
Fifty-five percent of patients suffered from severe and life-threatening asthma exacerbations, 63.6% of them were females. The most important predictors for severe exacerbations were SO2 < 90% at baseline (OR = 4.56; 95% CI = 3.45–7.56; P < 0.001), PEFR after 1 h (OR= 3.34; 95%CI = 1.90–4.90; P < 0.001), and uncontrolled asthma (OR= 3.33; 95%CI = 2.50–5.05; P < 0.001). Predictors for hospitalization were old age (OR = 1.11; 95%CI = 1.09–2.11; P < 0.001), uncontrolled asthma (OR = 2.34; 95%CI = 2.01–4.40; P < 0.001), PEFR after 1 h (OR= 4.44; 95%CI= 3.24–7.68; P < 0.001), and SO2 <90% at baseline (OR= 5.67; 95%CI= 3.98–8.50; P < 0.001).

Conclusions
Severe asthma exacerbations can be predicted by old age, previous history of mechanical ventilation, obstructive sleep apnea, overuse of SABA, uncontrolled asthma, moderate to severe depression, eosinophilia, SO2 <90%, and low peak expiratory flow rates.
Most Important Things and Associated Factors With Prioritizing Daily Life in Patients With Advanced Lung Cancer
Authors: Kameyama Naofumi , Sato Takashi , Arai Daisuke , Fujisawa Daisuke , Takeuchi Mari , Nakachi Ichiro , Kawada Ichiro , Yasuda Hiroyuki , Ikemura Shinnosuke , Terai Hideki , Nukaga Shigenari , Nakano Yasushi , Hirano Toshiyuki , Minematsu Naoto , Asakura Takanori , Kamatani Takashi , Tanaka Kyuto , Suzuki Shoji , Miyawaki Masayoshi , Naoki Katsuhiko , Fukunaga Koichi , Soejima Kenzo 
Published: November 2022

PURPOSE: Patients' values and priorities in their lives should be appreciated from an early phase of incurable diseases such as advanced cancer. However, studies examining these characteristics have been lacking. This study attempted to determine what patients with advanced lung cancer valued most, once they had been diagnosed, and any associated factors. METHODS: Patients with newly diagnosed advanced lung cancer (N = 248) were enrolled in a questionnaire survey conducted at 16 hospitals in Japan. Their priorities were assessed using a free-text response to the question what is the most important thing to you now? at the time of diagnosis and 3 months after diagnosis. The free-text responses were classified into 10 categories for quantification. The clinical characteristics associated with the category describing daily life were further examined. RESULTS: Free-text comments were obtained from 103 (44.0%) and 66 (42.6%) patients at the time of diagnosis and at 3 months, respectively. The most frequent categories were family (at diagnosis: 50.5%; at 3 months: 50.0%) and daily life (at diagnosis: 33.0%; at 3 months: 36.4%), followed by health (at diagnosis: 32.0%; at 3 months: 27.3%) at both time points. The patients mentioning daily life, the issues related to how to spend daily life, showed significantly higher total scores and functional well-being subscale scores on the Functional Assessment of Cancer Therapy-Lung scale at both time points and lower depression scores at diagnosis and lower anxiety scores at 3 months on the Hospital Anxiety and Depression Scale. CONCLUSION: Family and daily life were highly valued by patients with advanced lung cancer at diagnosis. A better quality of life and better mood were associated with mentioning daily life, which should be taken into account in care planning to maintain patients' involvement in daily life even with incurable diseases.
Incorporating reading strategies for EFL undergraduate learners in Saudi Arabia: A think-aloud study
Authors: Al-Ahdal Arif Ahmed Mohammed Hassan , Alolaywi Yasamiyan 
Published: November 2022

Background: In language learning, reading is a skill that enables interaction with a text in whatever field of knowledge the learner is pursuing. Readers tend to use strategies such as comprehension, interpretation and conception of decoding written language and texts to enhance their reading capacity. This research explores the reading strategies applied by Saudi English as a Foreign Language (EFL) learners and compares the reading abilities of male and female EFL students. Methods: The study interviewed three EFL students about the reading strategies they applied while reading passages and texts. Then an International English Language Testing System (IELTS) reading test was handed over to 26 randomly selected students. Results: The study found that the reading strategies used by the students interviewed involved skimming, scanning, guessing the meaning from context, identifying the mean idea, and summarizing the body of the text in question. Moreover, the study revealed that both male and female students scored low in the IELTS tests that the researchers conducted. The average mean score they reached was 7.15 out of 20. However, even in the low scoring ratio, female students (M=7.69) outperformed male students (M= 6.62) and the difference between them remained significant, P=.000. Conclusions: The study recommends that the language instructors help EFL learners in developing their reading strategies and applying them every time they read any text.