bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological characteristics of patients from fever clinics with COVID-19 epidemic in 2022 in Shanghai, China.
Authors: Zhang Yuanjing , Wang Jianrong , Xie Ying , Cao Xinghao , Huang Huili , Liu Qingyang , Hang Xiaofeng , Wang Junxue 
Published: November 2022

Abstract
The outbreak of COVID-19 in Shanghai, China, in March 2022, was caused by the Omicron variant. The epidemic lasted for more than 3 months, and the cumulative number of infected people reached 626,000. We aimed to investigate the impact of clinical factors on disease outcomes in patients with COVID-19. In this study, we collected fever clinics confirmed Omicron variant infection cases, analyzed their population and laboratory diagnostic characteristics, and provided theoretical support for subsequent epidemic prevention and control. The results of this study show that the COVID-19 vaccine can protect against Omicron variant infection, and more than 50% of infected people have not been vaccinated before. Compared with the epidemic in Wuhan 2 years ago, most of the patients in the hospital in the Shanghai epidemic had underlying diseases (P < 0.05). In the comparison between patients with Omicron variant epidemic in Shanghai and patients with respiratory tract infection, there was no significant difference in N%, L%, monocytes, eosinophils, RBC, HB, PLT, etc. (P > 0.05). People over 60 years old and with underlying diseases were the risk factors for pneumonia [HR: 14.62 (5.49–38.92), P < 0.001; HR = 5.29 (2.58–10.85), P < 0.001, respectively]. But vaccination was a protective factor [HR = 0.24 (0.12–0.49), P < 0.001]. In summary, it was confirmed that the vaccine has an effect on the infection of Omicron variant strains and the protection against pneumonia. The virus damage of Omicron variant strains has a significant downward trend compared with 2 years ago.
Representation of Racial and Ethnic Minoritized Groups in Preventive Intervention Research
Authors: BUCKLEY PAMELA , Murry Velma McBride , Gust Charleen J. , Ladika Amanda , Pampel Fred C. 
Published: November 2022

Abstract
Objective. Because racial and ethnic minoritized groups disproportionately represented essential workers and lacked equitable access to resources that mitigated exposure and mortality risk, the COVID-19 pandemic brought disparities to the forefront of public health, exacerbating existing discrepancies. These inequities highlight a pressing need for the prevention science field to investigate whether interventions promote equitable well-being, which served as the impetus for this study. We examined 885 programs with evaluations published from 2010-2021 and recorded in the Blueprints for Healthy Youth Development registry of preventive interventions.
Methods. Focusing on race and ethnicity but also tracking reporting of gender, location, and economic disadvantage, we conducted a descriptive analysis on the prevalence of culturally tailored interventions (i.e., those developed for specific populations) and reporting of sample characteristics. In addition, inferential analyses examined reporting time trends, as well as the relationship between study quality (i.e., methodological soundness, beneficial effects) and culturally tailored programs, and racial ethnic enrollment.
Results. Most studies were conducted in the U.S. (n=583). Two percent of programs were developed for Black or African American youth and four percent targeted Hispanic or Latino populations. For the 77% of studies that reported race, most enrollees were White (35%) followed by Black or African American (28%), and 31% were collapsed across racial categories or categorized with ethnicity, thus ignoring the intersectionality of race and ethnicity. In the 64% of studies that reported ethnicity, 32% of enrollees identified as Hispanic or Latino. Reporting did not improve over time, and there was no relationship between high quality evaluations and programs developed for racial ethnic minority groups, or samples with high proportions of racial ethnic enrollees.
Conclusions. Research gaps on racial and ethnic groups identified in this study indicate the need for improved representation and clear reporting to reduce disparities and improve the utility of preventive interventions.
Blood Profile of West African Dwarf Growing Bucks Fed Fungal Treated Megathyrsus Maximus and Brachiaria Decumbens Based Diets.
Authors: Idowu Oluwaseun Janet , Chaudhry Abdul Shakoor , Tanimowo Razaq Olaide , Adelusi Olusegun Oludotun , Ojo Victoria Olubunmi Aderemi , Akinyemi Stephen Boluwatife , Agboola Feranmi Oyinlola , Ogunsiji Racheal Tunmise 
Published: November 2022

Abstract
Fungal treated grasses can be used as a supplementary feed for ruminants during dry seasons. A total of 24 West African dwarf (WAD) growing bucks with the average weight of 7 kg were fed with fungal treated either Brachiaria decumbens (TBD) or Megathyrsus maximus var. ntchisii (TMMN) to investigate their effects on the animals’ blood profile. The goats were allocated to six dietary treatments in a completely randomized design. The diets were offered as a total mix ration (100%) with one of the two treated forages at one of three levels (0%, 20%, 30%). Additionally, each diet contained concentrate (50%) and cassava peels (either 50% or 30%, or 20%) to a total of 100%. These diets were fed to the animals for 21 days with 14 days adaptation and the last 7 days for data collection. It appeared that the diets significantly (P < 0.05) influenced some haematological and serum biochemical parameters. The fungal treated BD and MMN at 30% inclusion maintained the animals within the expected ranges of blood profiles, indicating no apparent threat to goat wellbeing. However, animals consuming TBD diet showed better health than those fed TMMN. Therefore, 30% inclusion of TBD can be used as an alternative feedstuff in ruminant diets during the dry season.
Profile of Positive Specific Immunoglobulin E to Aeroallergen among Sudanese Patients at Al-Rayan Laboratory from January 2019 to December 2020
Authors: Mohamedahmed Mohamed Hassan Osman , Salih Omaima Abdelmajeed Mohammed , Mustafa Alamin , Mohammed Hala Hamza Eltayeb , Hassan Malaz Elsadeg , Ahmed Rogaia Hasap Alrasoul 
Published: November 2022

Abstract
Introduction:
Aeroallergens are various airborne substances or inhalants, such as pollen, spores, and other biological or non-biological airborne particles that can cause allergic disorders. Inhalation or cutaneous contact with aeroallergens can trigger a release of proteins in the form of an allergic reaction on the skin and mucous membranes.
Methods: This study was a qualitative retrospective study conducted in the Al-Rayan lab in Khartoum state from January 2019 to December 2020. All records with positive specific IgE to aeroallergens have been collected from Al Rayan Laboratory center. The immunoblotting technique [Euroline food gulf (IgE)] detect the IgE antibody against specific aeroallergens
Result: . Ninety-nine participants were positive for the specific IgE test aeroallergens panel. Most of them were adults, and the mean age of the participants was 21.3 ± 16.3. The most prevalent aeroallergen was Timothy grass and Oak. Timothy grass was positive among (46.5%) of the patients, Cultivated rye, olive tree, and common ragweed were mainly positive in November. The test for Oak allergen came positive in November 2020, March 2020, and October 2019 with the respective percentages (64.3%, 85.7%,46.2%). The olive tree and common ragweed aeroallergen reactions came equally positive in November 2020 the most (57.1%). Moreover, the age of the participants was related to Dog and Horse aeroallergens. In addition to penicillium, children mainly tested positive for Dog, Horse aeroallergens at P values <0.05.
Conclusion:
This study demonstrates that the most common aeroallergens are Timothy grass and Oak, followed by cultivated rye.
VMD and Self-attention Mechanism Based Bi-LSTM Model for Fault Detection of Optical Fiber Composite Submarine Cables
Authors: Lu Jie , Feng Wenjiang , Li Yuan , Zhang Juntao , Zou Yongqi , Li Jingfu 
Published: November 2022

Abstract
As the main electrical equipment of offshore power grids, optical fiber composite submarine cables undertake the task of power transmission and data communication. In order to ensure the proper functioning of the submarine cable, it is necessary to analyze the working state of it and identify and locate the fault event. This paper proposes a fault detection method for submarine cables, that is, the VMD and self-attention based Bi-LSTM model. First, we use the ANSYS software to generate the vibration waveforms of three main fault events of optical fiber composite submarine cables. Then by generating the detection matrix of additive white gaussian noise (AWGN) and the vibration waveforms, it can realize the orientation and detection of fault events in single submarine cable. In addition, the vibration signal can be decomposed into IMF components using variational mode decomposition (VMD) for feature extraction. Moreover, the IMF components are input to the self-attention layer for feature fusion and Bi-LSTM module for further feature extraction. Finally, the result of the fault detection is output through the classification layer. According to the comparative experiment and ablation experiment, the proposed model has proved to outperform the other benchmark models and is robust and stable under the condition of different signal-to-noise ratios (SNRs).
Development and Validation of a Model for early Diagnosing Biliary Atresia.
Authors: Gong Zongrong , Lu Gen , Wan Chaomin 
Published: November 2022

Abstract
Background and aims: Early diagnosis of biliary atresia (BA), particularly distinguishing it from other causes of neonatal cholestasis (NC), is challenging. This study aimed to design and validate a predictive model for BA by using the data available at the initial presentation.
Methods: Infants presenting with NC were retrospectively identified from tertiary referral hospitals and constituted the model design cohort (n=148), and others were enrolled in a prospective observational study and constituted the validation cohort (n=21). Clinical, laboratory, and abdominal ultrasonographic features associated with BA were assessed. A prediction model was developed using logistic regression and decision tree (DT) analyses.
Results: Three predictors, namely, gamma glutamyl transpeptidase (γGT) level, triangular cord sign (TC sign), and gallbladder abnormalities were identified as the factors to diagnose BA in multivariate logistic regression, which was used to develop the DT model. The area under the receiver operating characteristic (ROC) curve (AUC) value for the model was 0.905, which was greater than those for γGT level, TC sign, or gallbladder abnormalities alone in the prediction of BA.
Conclusion: A simple prediction model combining liver function and abdominal ultrasonography findings can provide an accurate and early estimate of the risk of BA in patients with NC.
Comparation of in vitro fibril-forming properties and antioxidant activities of collagen extracted from four by-products (skin, fin, cartilage, notochord) of sturgeon (Acipenser gueldenstaedti)
Authors: Meng Dawei , Wei Qiwei , Takagi Yasuaki , Dai Zhiyuan , Zhang Yan 
Published: November 2022

Abstract
To efficient utilize sturgeon by-products, we clarified and compared the structural and biological properties of collagens extracted from fin, skin, notochord, and cartilage of sturgeon. The structural properties of collagen were analyzed by SDS-PAGE and FTIR. The in vitro biological properties of four sturgeon collagen were evaluated from fibril-forming ability of collagen molecule and the antioxidant activity of low molecular collagen peptides. Collagen fibril-forming ability and morphology were detected by turbidity assay and SEM observation. The antioxidant activities of collagen peptides were determined by Hydroxyl and ABTS radical scavenging assay. SDS-PAGE results showed that the main type of skin and fin collagen was attributed to type I collagen, and that of cartilage and notochord collagen was attributed to type II collagen. FTIR results revealed four collagens maintained intact triple helical structure. Type II collagens could self-assemble into fibrils only at low phosphate ion concentration, whereas type I collagens could self-assemble into fibrils at long range of phosphate ion concentration. The fibril diameter of type I collagen was higher than that of type II collagen. The antioxidant activity of notochord and skin collagen peptides was higher than that of the other two collagen peptides. The results of this study will provide helpful information for the application of sturgeon collagen in the functional food and biomedical material industries. Meanwhile, it will promote the effective use of collagen from different sturgeon by-products.
A novel thrombospondin-1 transcript exhibits distinctive expression and activity in thyroid tumorigenesis
Authors: Baek Seung Joon , Hong Yukyung , Kim Ilju , Moon Hyunjin , Lee Jaehak , Lertpatipanpong Pattawika , Ryu Chang Hwan , Jung Yuh-Seog , Seok Jungirl , Kim Yonghwan , Ryu Junsun 
Published: November 2022

Abstract
Thrombospondin 1 (TSP1) is known for its cell-specific functions in cancer progression, such as proliferation and migration. It contains 22 exons that may potentially produce several different transcripts. Here, we identified TSP1V as a novel TSP1 splicing variant produced by intron retention (IR) in human thyroid cancer cells and tissues. We observed TSP1V functionally inhibited tumorigenesis contrary to TSP1 wild-type (TSP1W), as identified by in vivo and in vitro. These activities of TSP1V caused by inhibiting p-Smad and p-FAK. RT-PCR and minigene experiments revealed that some phytochemicals/non-steroidal anti-inflammatory drugs (NSAIDs) enhanced IR. We further found that RNA binding motif protein 5 (RBM5) suppressed IR, induced by sulindac sulfide treatment. Sulindac sulfide also reduced p-RBM5 in a time dependent manner. On the other hand, trans-chalcone demethylated TSP1V, thereby preventing MeCP2 binding to TSP1V gene. In addition, TSP1V levels were significantly lower in patients with DTC, but not in those with BTN, indicating its potential application as a diagnostic biomarker in tumor progression.
Development of a Low-Cost Insulin Infusion Pump Simulator:A Case Study with a Brazilian Company
Authors: Rocha Felipe dos Santos , Martins Luiz Eduardo Galvão , de Oliveira Tiago , Aredes Sebastião Vagner , Dib Sergio Atala , Casarini Dulce Elena , Colucci Juliana Almada , Gabbay Monica Andrade Lima , Cunha Tatiana Sousa 
Published: November 2022

Abstract
Purpose
The purpose of this paper is to present and illustrate the development of a low-cost insulin infusion pump simulator. Methods: The simulation was written entirely using Java programming language, reproducing the appearance and functions of a low-cost insulin infusion pump. Later, the participants were invited to participate in a remote session and based on the results of a standard usability scale (SUS), it was possible to draw the assessment and check the system’s readiness. Results: By means of a sample size of 34 participants with different levels of knowledge on diabetes and infusion pumps, we found an average SUS score of 67.43, which is not directly linked to previous knowledge of the users. Conclusion: Although the insulin infusion pump is a complex and highly dedicated equipment, and its system may seem unfamiliar at first, the usability score found is satisfactory and with proper training and clinical support it may be improved, enabling the equipment to fulfill its purpose efficiently. Significance: The use of a simulation model during the development of an insulin infusion pump prototype may bring some benefits, paving the way for a well-defined, robust and safe system, improving the delivery of health care technologies and increasing patient safety.
Hysterectomy and risk of epithelial ovarian cancer by histologic type, endometriosis, and menopausal hormone therapy
Authors: Ring Linea Landgrebe , Baandrup Louise , Gottschau Mathilde , Dehlendorff Christian , Mellemkjær Lene , Kjaer Susanne K. 
Published: November 2022

Abstract

Purpose
This nationwide, register-based case-control study investigated the association between hysterectomy and risk of epithelial ovarian cancer according to histology and by history of endometriosis and MHT use.
Methods
From the Danish Cancer Registry, all women registered with epithelial ovarian cancer during 1998‒2016 were identified (n = 6,738). Each case was sex- and age-matched to 15 population controls using risk-set sampling. Information on previous hysterectomy on benign indication and potential confounders was retrieved from nationwide registers. Conditional logistic regression was used to calculate odds ratios (ORs) with 95% confidence intervals (CIs) for the association between hysterectomy and ovarian cancer according to histology, endometriosis, and use of MHT.
Results
Hysterectomy was not associated with risk of epithelial ovarian cancer overall (OR = 0.97; 95% CI 0.88‒1.06) but was associated with reduced risk of clear cell ovarian cancer (OR = 0.47; 95% CI 0.28‒0.78). In stratified analyses, decreased ORs associated with hysterectomy were seen in women with endometriosis (OR = 0.73; 95% CI 0.50‒1.09) and in non-users of MHT (OR = 0.87; 95% CI 0.76‒1.00).
Conclusion
Hysterectomy was not associated with epithelial ovarian cancer overall but with reduced risk of clear cell ovarian cancer. Our findings may suggest a reduced risk of ovarian cancer after hysterectomy in women with endometriosis and in MHT non-users, but the statistical precision of our results did not allow firm conclusions.
KAT8-catalyzed lactylation contributes to eEF1A2-mediated protein synthesis and colorectal carcinogenesis
Authors: Xie Bingteng , Zhang Mengdi , Li Jie , Cui Jianxin , Zhang Pengju , Liu Fangming , Deng Weiwei , Ma Jihong , Li Xinyu , Pan Bingchen Pan , Zhang Baohui , Zhang Hongbing , Luo Aiqin , Xu Yinzhe , Li Mo , Pu Yang 
Published: November 2022

Abstract
As an emerging metabolite-mediated post-translational modification, lysine lactylation (Kla) is discovered on histones that regulates gene transcription from chromatin. However, whether lactylation could occur outside the nucleus across broad proteins and its regulatory enzymes remains unclear. Here, we observe global Kla occurs in both nucleus and cytoplasm in colorectal cancer (CRC), and negatively correlates with its prognosis. Among the lactylated proteins in CRC, we find eEF1A2, a fundamental protein in translation elongation, is hyperlactylated at its lysine 408 (K408). The lactylation of eEF1A2K408 results in increased translation elongation rates and elevated protein synthesis in line with CRC cells proliferation. By screening eEF1A2 interacted proteins, we identify KAT8 as a pan-Kla writer which is responsible for installing Kla on many proteins in diverse biological processes, notably in tumorigenic signaling pathways. Deletion of KAT8 effectively decreases CRC tumor growth especially in high-lactic tumor microenvironment, which associates with reduced KAT8-mediated Kla. Together, our work uncovers that KAT8 catalyzes broad lactylation as a lactyltransferase which could be a potential target for CRC and the KAT8-eEF1A2 Kla axis is a novel mechanism for cancer adaptation to increased translational requirements.
Assessing the changes in protein content during the ensilage of the Indian mackerel (Rastrelliger kanagurta) by biological method
Authors: Mahale Adhira , Khandeparker Rakhee , Gauthankar Mukund , Paingankar Mandar , R.A. Sreepada 
Published: November 2022

Abstract

Fish silage is a liquefied product resulting from tissue hydrolysis and the richness of the silage is characterized by its nutritional profile comprising of proteins, amino acids, and fats. For the production of fish silage of high nutritional value, a precise assessment of the degree of hydrolysis, particularly proteolysis becomes a paramount importance. The goal of this study is to assess the changes in protein content in fish silage prepared from the Indian mackerel, (Rastrelliger kanagutra) waste using 10% (v/w) sugarcane molasses, during 8 days of fermentation (DoF). The resultant ensiled product was evaluated for changes in titratable acidity, nutrients, total protein, total carbohydrate, total lipids content, and amino acid composition. Altogether, 17 types of amino acids were identified. Concentrations of total amino acids (15.627 ± 0.03 mg/g) and free amino acids (5.556 ± 0.08 mg/g) peaked at the end of 6 DoF with the predominance of histidine, arginine, alanine, asparagine, aspartic acid, and glutamic acid. Results of the non-metric multidimensional scaling (nMDS) plots and one-way analysis of similarities (ANOSIM) test indicated significant differences in the composition of total amino acids at different DoF were discernible (nMDS, stress 0.03162, one-way ANOSIM R = 1; p < 0.0001). Similarity percentage (SIMPER) analysis indicated that maximum content of amino acids (TAA and FAA) were released in silage during 4–6 DoF. A significant difference was observed in concentrations of most amino acids except cysteine, serine, and tryptophan (ANOVA, p < 0.05) at 2,4,6 and 8 DoF. Considering the vital role played by amino acids in providing resistance to plant pathogens as well as contributing to animal nutritional supplements, the results of the present study are highly noteworthy.
GPs’ prescription patterns, experience, and attitudes towards medicinal cannabis - a nationwide survey in Denmark
Authors: Rosenbaek Frederik , Riisgaard Helle , Nielsen Jesper Bo , Wehberg Sonja , Waldorff Frans Bach , Pedersen Line Bjørnskov , Søndergaard Jens 
Published: November 2022

Abstract
Background: On 1 January 2018 a four-year test scheme concerning use of medicinal cannabis (MC) was enacted. It has recently been extended for four more years by the Danish Parliament permitting all Danish physicians to prescribe MC to their patients. Previous studies have shown that general practitioners (GPs) have varying prescription experience, little knowledge, and mixed attitudes about MC. However, the present evidence is still limited, and no studies exist about Danish GPs’ prescription experience, knowledge, and attitudes towards MC. Therefore, our aim was to examine Danish GPs’ prescription experience, knowledge, and attitudes towards MC.
Methods: A national online survey-based study addressing Danish GPs was performed from September 2018 to July 2019. We performed separate multivariable logistic regression analyses including GPs’ prescription experience, knowledge, and attitudes towards MC as outcome variables.
Results: A total of 427 (38.5%) of 1108 GPs completed the questionnaire. Of these, 37 (8.7%) had experience in prescribing MC. The majority had little or no knowledge about MC (80.6%) as well as a negative view on prescription of MC (71.4%) to patients. Factors associated with prescribing MC to patients were: Single-handed practices (OR=1.6, 95% CI 1.1;1.8) and perception of having quite some knowledge about MC (OR=4.8, 95% CI 2.2;10.4). Factors associated with having quite some knowledge about MC were: having a positive attitude towards prescribing MC (OR=5.2, 95% CI 1.9;14.0), being male (OR=1.7, 95% CI 1.4;1.8), and being at least 60 years of age (OR=2.8, 95% CI 1.3;6.0). Factors associated with having a positive attitude towards prescribing MC were: having quite some knowledge about MC (OR=5.2, 95% CI 2.2;12.5) and GPs being male (OR=1.7, 95% CI 1.1;1.9).
Conclusion: In this first study on prescription experience, knowledge, and attitudes about MC among Danish GPs, conducted one year after the Danish test scheme was enacted, we find a very low proportion of prescribers, little knowledge, and an overall negative attitude towards MC. Among the prescribing GPs, four in ten have little to no knowledge and a negative attitude towards MC. We stress that prescribing patterns, knowledge, and attitudes may change throughout the remaining time of the test scheme.