bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interfering with plant developmental timing promotes susceptibility to insect vectors of a bacterial parasite
Authors: Huang Weijie , Hogenhout Saskia A. 
Published: March 2022

SummaryMulti-trophic parasites often modulate host behaviors and development to attract other hosts. However, whether these modulations are controlled by specific parasite genes and are adaptive remains debated. Insect-transmitted phytoplasma parasites take control of their plant host by turning them into ‘Zombie plants’, which feature spectacular vegetative organ proliferations and juvenilization. The phytoplasma effector SAP05 induces these symptoms by mediating the degradation of multiple plant SPL and GATA developmental transcription factors via recruiting the ubiquitin receptor RPN10. Here, we investigated if these SAP05-induced modulations impact the leafhopper vectors on which phytoplasmas depend for spread. SAP05 promotes leafhopper reproduction on Arabidopsis thaliana in a RPN10-dependent manner. Moreover, SAP05 homologs that degrade SPLs and both SPLs and GATAs promote leafhopper reproduction, whereas those that degrade only GATAs do not. Leafhoppers also produced more progeny on plants with misregulated MiR156, which suppresses SPL expression. SPLs control several developmental processes. Surprisingly, leaf color and shape changes and increased leaf numbers did not correlate to leafhopper progeny increases, whereas disturbing the plant phase transition processes did. Therefore, only specific phenotypes induced by a single parasite gene extend beyond one host to control another and promote host-host attractions of a multi-trophic parasite.
High fat diet initiates rapid adaptation of the intestine
Authors: Enriquez Jacob R. , McCauley Heather A. , Zhang Kevin X. , Sanchez J. Guillermo , Kalin Gregory T. , Lang Richard A. , Wells James M. 
Published: March 2022

SummaryWhile the systemic impacts of overnutrition are well-known to cause obesity and metabolic syndrome over the course of months, the immediate adaptive response of the intestinal epithelium to dietary changes remains poorly understood. Here we used physiological metrics and single cell analyses to interrogate the adaptive response of intestinal epithelial cells when moved to a high fat diet (HFD). Within 1 day of HFD exposure, mice exhibited altered feeding behavior, an increase in energy expenditure and an increase in intestinal epithelial proliferation. Single cell transcriptional analysis demonstrated several acute cellular changes on day 1, including a cell-stress response in intestinal crypts, de-granularization of Paneth cells, and a shift towards fatty acid cellular metabolism. By 3 days of HFD, there was an emergence of uncommitted progenitors with a transcriptional profile indicative of a shift from secretory populations and towards an absorptive fate. In enterocytes, genes regulating lipid transport and absorption increased over the first 3 days which paralleled a functional increase in lipid absorption in vivo over the course of 7 days on HFD. These findings demonstrate that intestinal epithelial cell populations respond rapidly to changes in diet through initial changes in cellular function followed by a shift in cellular composition.
Structure-function conservation between the methyltransferases SETD3 and SETD6
Authors: Elisha Lee , Abaev-Schneiderman Elina , Cohn Ofir , Shapira Guy , Shomron Noam , Feldman Michal , Levy Dan 
Published: March 2022

AbstractAmong the protein lysine methyltransferases family members, it appears that SETD6 is highly similar and closely related to SETD3. The two methyltransferases show high similarity in their structure, which raised the hypothesis that they share cellular functions. Using a proteomic screen, we identified 52 shared interacting-proteins. Gene Ontology (GO) analysis of the shared proteins revealed significant enrichment of proteins involved in transcription. Our RNA-seq data of SETD6 KO and SETD3 KO HeLa cells identified ∼100 up-regulated and down-regulated shared genes. We have also identified a substantial number of genes that changed dramatically in the double KO cells but did not significantly change in the single KO cells. GO analysis of these genes revealed enrichment of apoptotic genes. Accordingly, we show that the double KO cells displayed high apoptotic levels, suggesting that SETD6 and SETD3 inhibit apoptosis. Collectively, our data strongly suggest a functional link between SETD6 and SETD3 in the regulation of apoptosis.
Effects of customer characteristics and service quality on share of wallet in neighbourhood shops based on an asymmetric approach
Authors: Osakwe Christian Nedu 
Published: March 2022

Purpose
The purpose of this paper is to increase the understanding of customer share of wallet (SoW) based on research in customer characteristics and the retail service quality model. Specifically, this study adopts an asymmetrical modelling approach to provide insights into the necessary and sufficient conditions leading to increased customer SoW in the context of neighbourhood shops in Southern Africa.

Design/methodology/approach
This study uses fuzzy-set qualitative comparative analysis to analyse survey data collected from 523 current customers of the shops.

Findings
This study reveals that large household size and low income are necessary conditions for increased SoW. It also reveals five unique customer profiles, or casual recipes, associated with increased customer SoW. More generally, this study demonstrates that service quality constituents (personal interaction, reliability, policy and physical aspects of the shop) and customer characteristics, namely, relationship duration, household size, gender and income-level act in combination to lead to customer SoW.

Originality/value
This study illustrates how service quality constituents and customer characteristics compete and/or complement each other in relation to increased customer SoW. To the best of the author’s knowledge, this is the first study to provide evidence on the necessary conditions for increased SoW, especially in the neighbourhood shop context of a developing economy. Value-wise, this paper provides a more nuanced perspective to understanding how unique customer profiles are associated with increased SoW.
Impacts of economic and social motivations on makers' exploitation and exploration activities in makerspaces
Authors: Yang Zhi , Lu Hui , Bao Jiaxin 
Published: March 2022

PurposeMakerspaces, which serve as fertile grounds for makers' innovation activities, are rapidly increasing in emerging markets to help unleash a massive wave of bottom-up innovation and encourage broader participation in entrepreneurial activities. Makers' motivations to innovate are key antecedents of their subsequent innovative behavior. The paper aims to investigate the impact of makers' innovation motivations (both economic and social motivations) on their exploration and exploitation activities in makerspaces and the moderating role of the makerspace climate for innovation.Design/methodology/approachA survey was conducted for 139 individual makers from five makerspaces in China to test the research hypotheses.FindingsEconomic motivation positively affected the degree of exploitative innovation and was negatively related to the degree of exploratory innovation. In contrast, social motivation negatively affected the degree of exploitative innovation and was positively related to the degree of exploratory innovation. The makerspace climate for innovation strengthened the relationship between social motivation and exploratory innovation and exacerbated the negative effect of economic motivation on exploration.Practical implicationsThe results offer managers a better understanding of how makers' motivation to participate in makerspaces affects their innovative behavior. Such information can guide makerspaces in designing their incentive policies and recruiting makers in line with their values to amplify makers' creative potential.Originality/valueThe empirical results reveal the impacts of economic and social motivations on makers' exploration and exploitation activities in makerspaces. They thus provide new insights into how different motivations give rise to different innovative behaviors and imply how makers' innovation activities can be managed effectively.
Regulatory reform and the regulatory state in the post-COVID-19 world
Authors: Tomic Slobodan , Heims Eva 
Published: March 2022

PurposeReflecting on recent empirical developments as well as insights from regulatory state theory, the paper considers directions in which the regulatory state could develop in the post-COVID-19 era.Design/methodology/approachThis is a de-contextualised analysis of regulatory developments drawing on the prior regulatory state literature and literature on post-crisis responses. Taking into account recent empirical developments related to the COVID-19 pandemic, the paper sets out, in a comparative context, scenarios for the future development of the regulatory state.FindingsPredicting the direction in which the regulatory state will develop is challenging, particularly at this early stage. Yet, we provide a conceptual framework for thinking about possible futures of the regulatory state and how domestic and international factors might mediate these futures.Originality/valueThe paper provides a structured approach to the analysis of the regulatory state bringing together insights from the literature on the regulatory state, public management reform, and global regulatory shifts.
Generating shared value: intersection between organisations that invest in social innovation
Authors: Gonçalves Malcon Santos , d'Angelo Marcia Juliana , Rocha Raysa Geaquinto 
Published: March 2022

PurposeThis study aims to investigate how organizations that invest in social innovation – such as for-profit and social enterprises – organize and shape the dynamics between social innovation and transformation.Design/methodology/approachThis is a qualitative interpretative study through the theoretical lens of social innovation. The organizational phenomenon studied was the business strategy for sustainable regional development of a Brazilian financial institution. Secondary data were collected from financial institution’s documents. The data analysis followed a categorization technique.FindingsThis research presents five intersectional elements that act as agglutinating factors between the structural elements common to these organizations: the processes (inter-organizational and intra-organizational), the forms of materialization of social innovation (products, experiences and replicable forms) and the impacts (in society and organization).Practical implicationsThe paper contributes to the debate about social innovation as to its definition, showing that it is both a phenomenon and a theory, which is still little explored. In particular, in emerging countries, such as Brazil, which publishes little about social innovation in international literature, going beyond European countries. Second, an empirical deeper understanding of the for-profit “organizations” modus operandi adopts and integrates social innovation in their strategies and operations. Third, given the multidimensional and complex nature of the social systems involved in innovation actions, it discusses a strategy that acts as an agglutinating factor. Finally, with reflections so that managers can discuss and start up a social innovation project.Originality/valueMost research is based on nonempirical evidence. Among the few empirical works, the focus is on the performance of nonprofit organizations in the promotion, development and implementation of projects involving social technology. The literature does not adequately understand how for-profit organizations adopt and integrate social innovation into their strategies and operations. Furthermore, it is observed that research analyzes data from European countries, with few approaches eying countries in development.
Non‐invasive neuromodulation can reduce aggressive behaviors in humans: A critical perspective
Authors: Volpe Giuseppe , Tagliente Serena , Palmisano Annalisa , Grattagliano Ignazio , Rivolta Davide 
Published: March 2022

AbstractContaining aggressive behavior is an ongoing challenge in modern society. Aggressiveness is a multi‐level construct that can be driven by emotions (reactive aggression) or can be “cold‐blooded” and goal‐directed (proactive). Aggressive behavior could arise because of a misjudgment of others' intentions or can follow frontal brain lesions leading to a reduction of impulse control and emotion regulation. In the last few years, interventional and basic research studies adopting Non‐Invasive Brain Stimulation (NIBS) have significantly risen. Those techniques have been used both in healthy people, to better understand the role of certain brain regions in psychological processes, and in aggressive subjects to improve their symptoms. From an overview of the literature, focusing on the paper that uses transcranial direct current stimulation (tDCS) to reduce aggressiveness, it emerges that tDCS can (i) enhance facial emotion expression recognition, (ii) improve impulses control, and (iii) affect approach/withdrawal motivation. The current work shows the strengths and weaknesses of tDCS intervention on aggressive individuals, suggesting that this instrument could be adopted on violent people, and paves the way for intervention in some applied settings such as prison.
Dialysis and extracorporeal therapies for enhanced elimination of toxic ingestions and poisoning
Authors: Yaxley Julian , Scott Tahira 
Published: March 2022

AbstractPoisoning and toxic ingestions cause significant morbidity and mortality worldwide. Extracorporeal therapies such as dialysis, hemoperfusion, and plasma exchange are selectively applied to patients with severe intoxications unresponsive to standard interventions and can be lifesaving. Extracorporeal therapies are a complex but fundamental aspect of the practice of nephrology. Without high‐quality evidence to guide implementation, an understanding of toxicokinetics and the physiochemical principles of the enhanced elimination techniques is especially important. This review provides a comphrensive, user‐friendly outline of the application of extracorporeal therapy in the poisoned patient.
Immunotherapy and Transarterial therapy of HCC: What the interventional radiologist needs to know about the changing landscape of HCC treatment?
Authors: Tibballs Jonathan , Clements Warren 
Published: March 2022

AbstractHepatocellular carcinoma (HCC) is the fourth most common cancer worldwide and its incidence is increasing in Australia. Transarterial therapy, predominantly transarterial chemoembolization (TACE) but increasingly transarterial radioembolization (TARE), plays an important role in patients with intermediate‐stage disease and preserved liver function. However, despite advances in TACE, TARE and adjunctive procedures, overall survival has only modestly increased over the last 20 years. Immunotherapy has emerged as a newer cancer treatment and uses antibodies directed at checkpoint inhibitors to upregulate T‐cell mediated tumour‐specific death. These drugs have been shown to increase survival in patients with HCC and have changed the landscape for advanced disease. Trials are now ongoing combining transarterial therapy and immunotherapy. This manuscript introduces these trials and interventional radiologists should be aware of the changing landscape so that they can partner with immunotherapy and remain relevant in the HCC multidisciplinary group as immunotherapy use increases.
Association between clinical outcomes and local treatment in stage IV non‐small cell lung cancer patients with single extrathoracic metastasis
Authors: Lim Jeong Uk , Kang Hye Seon , Shin Ah Young , Yeo Chang Dong , Park Chan Kwon , Lee Sang Haak , Kim Seung Joon ,   Korean Association for Lung Cancer, Korea Central Cancer Registry
Published: March 2022

AbstractBackgroundLocal treatment (LT) such as radiotherapy and metastasectomy on metastatic sites may improve outcomes in oligometastatic NSCLC patients, but more data are necessary to support LT in oligometastatic diseases. Patients with single extrathoracic metastatic lesion are more likely to benefit from local therapy. In this study, we evaluated the impact of LT in NSCLC patients with a single extrathoracic metastatic lesion.MethodsData were obtained from the Korean Association for Lung Cancer Registry (KALC‐R), a database created using a retrospective sampling survey by the Korean Central Cancer Registry (KCCR) and the Lung Cancer Registration Committee.ResultsA total of 787 NSCLC patients with a single extrathoracic metastatic lesion were evaluated. In the multivariate analysis for OS, age, female sex, poor performance score, squamous histological subtype, LT, and initial treatment modality showed significant associations. Regarding LT, groups that underwent curative LT were significantly associated with better OS compared to groups that did not undergo LT (p = 0.011, HR 0.448, 95% CI: 0.242–0.829). In the multivariate analysis of patients who underwent LT, poor performance score, initial treatment modality, and T stage were independently associated with poor OS. Compared to the T1 stage, T3 stage showed an HR of 2.470 (95% CI: 1.309–4.663; p = 0.005) and T4 stage showed an HR of 2.063 (95% CI: 1.093–3.904; p = 0.026).ConclusionIn NSCLC with a single extrathoracic metastatic lesion, LT, especially for curative purposes, has an independent association with OS. Moreover, among the patients who received LT, factors such as T stage, poor performance score, and initial treatment modality were significantly associated with OS.
Land‐use alters the form of larval density dependence to increase extinction risk in a grassland amphibian
Authors: Kross C. S. , Willson J. D. 
Published: March 2022

AbstractAn understanding of interactions between anthropogenic stressors and intrinsic population drivers is needed to fully understand wildlife population declines. Density dependence is a key aspect of population regulation for many species, especially for species that have high reproductive potential, such as amphibians. However, patterns of density dependence have been characterized for only a few species and little work has evaluated how density‐dependent interactions may be altered by anthropogenic stressors. We combined the results of a mesocosm experiment with demographic population modeling to investigate how the conversion of native prairie to agricultural grasslands dominated by Tall Fescue grass (Lolium arundinacea) affected larval density dependence and adult population size of an imperiled amphibian, Lithobates areolatus (Crawfish Frog). Overall, density dependence was overcompensatory, suggesting that L. areolatus exhibits scramble competition as larvae. Both vegetation treatments had low survival at high densities, but more individuals survived to metamorphosis at moderate densities in Fescue treatments compared to Prairie treatments. We evaluated the implications of our experimental results using a stochastic density‐dependent matrix population model to project long‐term population dynamics. Simulated populations breeding in Fescue‐dominated wetlands had a more variable population size and up to 400% higher probability of quasi‐extinction within 200 years, compared to populations breeding in ponds with prairie vegetation. Without varying density in experimental treatments and using mathematical models to project emergent population dynamics, our mesocosm experiment results would have suggested a slightly positive effect of Fescue grass on amphibian development and survival. Vegetation changes surrounding breeding wetlands might play an important role in the decline of amphibian populations persisting in low‐intensity agricultural areas.
The use of active methodologies for the teaching of human embryology: A systematic review
Authors: Guilherme Guedert Denis , de Lima Paola , Souza e Silva Renata , Cláudia Carneiro Girão Carmona Virgínia 
Published: March 2022

AbstractEmbryology is a basic science, of the medical curriculum and of the health area, it serves as a foundation for the understanding of the phenomena that occur in normal development and its alterations. Over the years, active methodologies have been increasingly used as innovative tools in the academic training of health professionals. In this work we aim, through an integrative review, to search for the active methodologies used in the teaching of embryology. The PICo strategy was used to form the guiding question, the results were presented through the Prisma Flow diagram. The databases consulted were PubMed, Embase, Scopus, Web of Science, Science Direct, Medline, Scielo and LILACS, searching for articles until the year 2021. Our results show that embryology is a science still to be explored in the field of methodologies active, few works were found, most using TBL or digital platforms; however, the authors agree that active methodologies are valuable tools in the teaching of embryology, capable of improving student motivation and engagement.
Robot programming versus block play in early childhood education: Effects on computational thinking, sequencing ability, and self‐regulation
Authors: Yang Weipeng , Ng Davy Tsz Kit , Gao Hongyu 
Published: March 2022

AbstractProgrammable robotics is recently used in early childhood education (ECE) to introduce programming and computational thinking (CT) skills. However, there is a further need for research to contrast the efficacy of children's participation in robot programming and traditionally beneficial ECE activities. The present study thus investigated the effects of a robot programming intervention versus a block play program on kindergarteners' CT, sequencing ability, and self‐regulation. The experiment (robot programming) versus comparison (block play) condition was randomly assigned to four kindergarten classes, which included 101 kindergarteners (M = 64.78 months, SD = 7.64). Statistical analyses revealed that the robot programming group (N = 54) had experienced greater gains over time in sequencing ability relative to those in the block play group (N = 47; F = 5.09, p < 0.05). Children in the robot programming group with lower level of self‐regulation at baseline showed larger improvements in sequencing ability over time relative to the block play group (F = 2.37, p = 0.01). Also, children in the robot programming group with older age showed larger improvements in CT over time relative to the block play group (F = 2.40, p < 0.01). The study demonstrates the positive benefits of robot programming to early childhood development in terms of CT and sequencing ability, compared to a traditional curriculum activity in ECE—block play.
Practitioner notesWhat is already known about this topic

Screen‐free robot programming can enhance preschool children's computational thinking (CT).
Block play can enhance preschool children's mathematics and executive functioning.
Both robot programming and block play are engaging for preschool children.
What this paper adds

An unplugged CT assessment is used to measure and compare the effects of both robot programming and block play interventions among preschool children.
Robot programming outperforms block play in promoting children's school readiness skills such as sequencing ability.
Children with lower self‐regulation skills benefited more from the robot programming intervention.
Implications for practice and/or policy

Robot programming and CT education should be expanded in diverse early childhood settings to boost the positive effects.
Technology‐enhanced curricula should be integrated into early childhood education.
Teachers should receive training on robot programming in addition to more traditional skills such as scaffolding children's block play.