bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimising the implementation of a universal web-based mental health service for Australian secondary schools: A cluster randomised controlled trial
Authors: Subotic-Kerry Mirjana , Mackinnon Andrew , Gallen Dervla , Baker Simon , Parker Belinda Louise , Achilles Melinda Rose , Chakouch Cassandra , Cockayne Nicole , Christensen Helen , O’Dea Bridianne 
Published: February 2025

ABSTRACTBackgroundSecondary schools are increasingly delivering a range of mental health interventions with varied success. This trial examined the effectiveness of two implementation strategies, class time allocation, and financial incentives, on secondary students’ engagement with a universal web-based mental health service, ‘Smooth Sailing’.MethodsA three-arm, cluster-randomised trial was conducted over 12 weeks with Grade 8 and 9 students from 20 schools in two Australian states. The primary outcome was student engagement, measured by the number of modules accessed at 12-weeks post-baseline. Secondary outcomes included uptake, retention, help-seeking intentions for mental health problems, service satisfaction, and barriers to use.ResultsA total of 20 schools consented to take part and 1295 students consented to the study. The mean number of modules accessed by students was higher in the enhanced conditions (class time allocation and financial incentive) but not statistically significant (P>0.140). There were no significant differences in uptake (P=0.554) or retention (P=0.945) between conditions. Help-seeking intentions significantly improved at 6- and 12-weeks in the standard service and class time conditions only. Common barriers to service use among students were forgetfulness and low motivation.ConclusionsNone of the implementation strategies examined by this trial significantly increased student engagement when measured by modules accessed.Trial registrationAustralian New Zealand Clinical Trial Registry (ACTRN12621000225819) and Universal Trial Number (U1111-1265-7440).Trial fundingThe trial operations were funded by the Prevention Hub, Australian Government Department of Health.
Unsupervised Representation Learning of C. elegans Poses and Behavior Sequences From Microscope Video Recordings
Authors: Deserno Maurice , Bozek Katarzyna 
Published: February 2025

ABSTRACTCaenorhabditis elegans (C. elegans) is an important model system for studying molecular mechanisms in disease and aging. The nematode can be imaged in highly parallel phenotypic screens resulting in large volumes of video data of the moving worm. However converting the rich, pixel-encoded phenotypical information into meaningful, quantitative description of behavior is a challenging task. There is a range of methods for quantification of the simple body shape ofC. elegansand the features of its motion. These methods however are often multi-step and fail in the case of highly coiled and self-overlapping worms. Motivated by the recent development of self-supervised deep learning methods in computer vision and natural language processing, we propose an unbiased, label-free approach to quantify worm pose and motion from video data directly. We represent worm posture and behavior as embedding vectors and visualize them in a unified embeddings space. We observe that the vector embeddings capture meaningful features describing worm shape and motion, such as the degree of body bend or the speed of movement. Importantly, using pixel values directly as input, our method captures coiled worm behaviors which are inaccessible to methods based on keypoint tracking or skeletonization. While our work focuses onC. elegans, the ability to quantify behavior directly from video data opens possibilities to study organisms without rigid skeletons whose behavior is difficult to quantify using keypoint-based approaches.
Feed-forward loops by NR5A2 ensure robust gene activation during pre-implantation development
Authors: Kobayashi Wataru , Ruangroengkulrith Siwat , Nur Arslantas Eda , Mohanan Adarsh , Tachibana Kikuë 
Published: February 2025

AbstractThe pioneer transcription factor NR5A2 plays multiple roles in regulating zygotic genome activation and expression of lineage-determining factor during mouse pre-implantation development. However, how NR5A2 differentially regulates transcriptional networks at distinct developmental stages remains unknown. Here, we demonstrate the dynamics of chromatin binding profiles of NR5A2 from the 2-cell to morula stage, which corresponds to the totipotency-to-pluripotency transition. Our NR5A2 CUT&Tag analysis identifies lineage-determining factor KLF and GATA families as co-regulators for NR5A2 in mouse embryos. We find that KLF5 cooperates with NR5A2 to enhance H3K27ac deposition, and NR5A2 predominantly promotes chromatin accessibility. NR5A2 regulatesXistexpression either directly or indirectly through its role in up-regulating GATA factor expression.In vitroassays reveal that NR5A2 co-binds to nucleosomes with KLF5 and GATA6, suggesting that these pioneer transcription factors can simultaneously bind to the chromatin. Our findings highlight the role of NR5A2 in ensuring robust gene activation during pre-implantation development through feed-forward regulatory loops with lineage-determining transcription factors.
Curcumin-silk tyrosine crosslinked hydrogels
Authors: Sundarakrishnan Aswin 
Published: February 2025

AbstractSystemic chemotherapy is still the first-line treatment for cancer, and it’s associated with toxic side effects, chemoresistance and ultimately cancer recurrence. Rapid gelling hydrogels can overcome this limitation by providing localized delivery of anti-cancer agents to solid tumors. Silk hydrogels are extremely biocompatible and suitable for anticancer drug delivery, but faster gelling formulations are needed. In this study, we introduce a rapid gelling hydrogel formulation (< 3 minutes gelling time) due to chemical crosslinking between silk fibroin and curcumin, initiated by the addition of minute quantities of HRP and H2O2. The novel observation in this study is that curcumin, while being a free-radical scavenger, also participates in accelerating silk di-tyrosine crosslinking in the presence of HRP and H2O2. Using UV-Vis, rheology and time-lapse videos, we convincingly show that curcumin accelerates silk di-tyrosine crosslinking reaction in a concentration-dependent manner, and curcumin remains entrapped in the hydrogel post-crosslinking. FTIR results show an increase in secondary beta-sheet structures within hydrogels, with increasing concentrations of curcumin. Furthermore, we show that curcu-min-silk di-tyrosine hydrogels are toxic to U2OS osteosarcoma cells, and most cancer cells are dead within short time scales of 4 hours post-encapsulation.Graphical abstract
Modeling the Competitive Evolution and Large-scale Transmission Dynamics of SARS-CoV-2 and Emerging Viral Pathogens
Authors: Huang Sisi , Lu Xiangyun , Xiao Zhengyun , Zhuang Yiqi , Wei Xin , Fan Zuyan , Huang Lingtong , Zhao Shijie , Lynch Michael , Yao Hangping , Zheng Min , Jiang Chao 
Published: February 2025

AbstractA fundamental question in virology and epidemiology is how viral variants compete and evolve during large-scale transmission and what drives one variant to dominate—key to understanding pathogen emergence and mutation-driven fitness advantages. In this study, we address this by integrating epidemiological, clinical, and experimental data to examine the co-infection and competitive evolution of the earliest SARS-CoV-2 lineages, A and B, two key variants circulating in early 2020. To capture these dynamics, we developed the evolutionary-SIR-Seeding-Spreading (evoSSS) model, a holistic framework that integrates transmission, competition, and spatial bottlenecks. The model simulates viral competition across multiple scales, from intra-host replication to inter-host transmission and from regional outbreaks to global spread. It also predicts risks for other infectious pathogens, such as influenza and monkeypox, and clarifies the competitive interactions between SARS-CoV-2 and influenza viruses. This study provides valuable insights into viral competitive evolution and informs strategies for managing future pandemics.
High-throughput, unbiased single-molecule displacement mapping with deep learning reveals spatiotemporal heterogeneities in intracellular diffusivity
Authors: Xia Jiankai , He Yi , Zhang Zhipeng , Yan Jinhong , Wang Ruirong , Tang Mingxuan , Chen Jingye , Fan Jun , Chen Kun 
Published: February 2025

AbstractSingle-molecule displacement/diffusivity mapping (SMdM) has revolutionized the study of molecular motion in live cells by providing high spatial resolution insights. However, its utility is restricted by measurement biases and low throughput, making it challenging to capture temporal dynamics of intracellular diffusivity. Here we report a high-throughput single-molecule diffusivity microscopy approach (Hi-SMdM) that rapidly and unbiasedly maps nanoscale heterogeneities in molecular motion inside mammalian cells, enabling time-resolved imaging of local diffusivity dynamics. Hi-SMdM employs a self-supervised deep-learning denoising framework with a general noise model to effectively restore the scarce signals of fast-moving single molecules, while eliminating artifact motions without relying on spatial redundancies or temporal correlations. It then provides unbiased estimations of nanoscale diffusion coefficient and improve both throughput and temporal resolution by up to an order of magnitude. We demonstrate the versatility of Hi-SMdM through time-resolved mapping of the spatially heterogeneous diffusivity of free proteins in the cytoplasm. Hi-SMdM also unveils the temporal dynamics of intracellular diffusion under hypotonic conditions in live cells. Additionally, Hi-SMdM tracks the spatiotemporal dynamics of intraorganellar diffusivity during rapid rearrangements of the ER network and functional activities of mitochondria. Overall, Hi-SMdM provides exceptional opportunities for high-throughput and unbiased single-molecule diffusivity mapping with excellent spatiotemporal resolution.
A novel peptidoglycan deacetylase modulates daughter cell separation inE. coli
Authors: Hernandez-Rocamora Victor , Martorana Alessandra M. , Belloso Aitana , Ballesteros Daniel , Zaccaria Marta , Perez Amilcar J. , Iorga Bogdan I. , Abia David , Gray Joe , Breukink Eefjan , Xiao Jie , Pazos Manuel , Polissi Alessandra , Vollmer Waldemar 
Published: February 2025

ABSTRACTPeptidoglycan hydrolases facilitate bacterial cell wall growth by creating space for insertion of new material and allowing physical separation of daughter cells. InEscherichia coli, three peptidoglycan amidases, AmiA, AmiB and AmiC, cleave septal peptidoglycan during cell division. The LytM-domain proteins EnvC and NlpD activate these amidases either from inside the cell or the outer membrane: EnvC binds to the cytoplasmic membrane-anchored divisome components FtsEX, and NlpD and ActS are outer membrane lipoproteins. Here we report the identification of a novel periplasmic deacetylase called SddA that removes acetyl groups from denuded peptidoglycan glycan strands, the products of amidases. SddA is a substrate for the periplasmic protease Prc, suggesting regulation via protein degradation. ThesddAgene is co-expressed with the gene encoding EnvC, linking SddA function to amidase activation. Consistent with this link, the deletion ofsddAalleviates phenotypes associated with lack of amidase activation, while overexpression ofsddAalleviates phenotypes related to a defective Tol-Pal system and causes cell chaining due to reduced septum peptidoglycan cleavage unlessenvCis co-expressed. We present a model according to which SddA modulates the activation of the septum-splitting amidases during cell division.AUTHOR SUMMARYBacteria surround their cell membrane by the essential peptidoglycan (cell wall) layer to prevent bursting open due to their turgor. During cell division, bacteria produce a septum at midcell, which must be cleaved for daughter cells to separate. Here, we report the identification of a new enzyme, SddA, that modifies a particular type of peptidoglycan material, denuded glycan chains released during the splitting of septal peptidoglycan for daughter cell separation, in the Gram-negativeEscherichia coli. We propose a model in which SddA modulates a switch in the septal peptidoglycan splitting, ensuring splitting is activated from the cell membrane in the early stages of cell division and from the outer membrane in the late stages.
CA2/3-dependent stability of frontoparietal mnemonic representations predicts episodic deficits in human amnesia
Authors: Miller Thomas D. , Hickling Alice L. , Wu Yan I. , Zhou Joseph H. , Handel Adam E , Coutinho Ester , Pollak Thomas A. , Zandi Michael S. , Rosenthal Clive R. , Maguire Eleanor A. 
Published: February 2025

AbstractThe hippocampus reconstructs past experiences by integrating sensory, perceptual, and conceptual information across a cortico-hippocampal autobiographical memory network (AMN). Here, in 18 human participants with amnesia, we decoded the effects of bilateral focal hippocampal damage on distinct autobiographical representations using representational dissimilarity matrices (RDMs). Hippocampal pathology resulted in: (1) impaired generalised episodic memory retrieval RDM model fit in the left angular gyrus; and, (2) reduced distinct episodic memory RDM model fit and trial-by-trial voxel-representational stability in the right angular gyrus, right orbitofrontal cortex, and right inferior frontal gyrus. RDM model fits and mnemonic stability were predicted by total CA23 volumes. Trial-by-trial retrieval stability within the right orbitofrontal cortex and right inferior frontal gyrus predicted autobiographical memory performance, providing the first direct neural correlate between hippocampal dysfunction, altered mnemonic representations, and amnesia. The results demonstrate that hippocampal-mediated amnesia results from impaired mnemonic representational distinctiveness and stability within the AMN.
Direct double ionization of the He-like
B3+
ion by a single photon
Authors: Müller A. , Hillenbrand P.-M. , Wang S.-X. , Schippers S. , Bray I. , Kheifets A. S. , Lindroth E. , Reinwardt S. , Martins M. , Seltmann J. , Trinter F. 
Published: February 2025

Within a joint experimental and theoretical research project, double photoionization of the He-like B3+ ion by a single photon was studied in the energy range from approximately 350 to 1160 eV. With the parent-ion beam in the experiment containing both 1s21S ground-state (90.9%) and 1s2sS3 metastable B3+ ions (9.1%), two contributions to the apparent single- and double-ionization cross sections, σ34app and σ35app, respectively, are involved in the measurements. Ratios Y(5+)/Y(4+) of double- over single-ionization yields were experimentally determined. By multiplying the measured ratios by the known cross section σ34app, the apparent cross section σ35app for double ionization was inferred. Using the information thus obtained, the measured yield of B5+ ions produced from hydrogenlike B4+ could also be normalized. The results show good agreement with theoretical benchmark cross sections σ45 for single photoionization of B4+. Convergent close-coupling calculations were performed to determine double-photoionization cross sections σ35 for B3+(1s2) and B3+(1s2s) ions. The result for the ground state agrees very well with theoretical data obtained previously with an intermediate-energy R-matrix approach. Within the systematic uncertainty of the normalized experimental cross sections, calculated and measured data are in agreement with one another.

Published by the American Physical Society
2025
Steering of sub-GeV positrons by ultrathin bent silicon crystal for ultraslow extraction applications
Authors: Garattini M. , Annucci D. , Gianotti P. , Liedl A. , Long E. , Mancini M. , Napolitano T. , Raggi M. , Valente P. 
Published: February 2025

For the first time at the Beam Test Facility (BTF) of the DAΦNE accelerator complex at the Laboratori Nazionali di Frascati of INFN, 450 MeV positrons have been deflected with high efficiency, using the planar channeling process in a bent silicon crystal. The deflection angle obtained is beyond 1 mrad. This interesting result finds several applications for manipulation of this kind of beams, in particular for ultraslow extraction from lepton circular accelerators like DAΦNE. In this work, the experimental apparatus, the measurement procedure, and the experimental results are reported.

Published by the American Physical Society
2025
Photoproduction of the
Σ+
hyperon using linearly polarized photons with CLAS
Authors: Clark L. , McKinnon B. , Ireland D. G. , Glazier D. I. , Livingston K. , Rönchen D. , Acar A. G. , Achenbach P. , Alvarado J. S. , Amaryan M. J. , Armstrong W. R. , Atac H. , Baashen L. , Barion L. , Battaglieri M. , Benkel B. , Benmokhtar F. , Bianconi A. , Biselli A. S. , Booth W. A. , Bossù F. , Brinkmann K.-Th. , Briscoe W. J. , Brooks W. K. , Bueltmann S. , Cao T. , Capobianco R. , Carman D. S. , Celentano A. , Chatagnon P. , Ciullo G. , Cole P. L. , Contalbrigo M. , D'Angelo A. , Dashyan N. , De Vita R. , Defurne M. , Deur A. , Diehl S. , Djalali C. , Dugger M. , Dupre R. , Egiyan H. , El Alaoui A. , El Fassi L. , Eugenio P. , Fegan S. , Ferguson R. F. , Filippi A. , Fogler C. , Gates K. , Gavalian G. , Gilfoyle G. P. , Golubenko A. A. , Gothe R. W. , Guidal M. , Hakobyan H. , Hattawy M. , Hauenstein F. , Hayward T. B. , Heddle D. , Hobart A. , Holtrop M. , Isupov E. L. , Jenkins D. , Jiang H. , Jo H. S. , Keller D. , Khandaker M. , Kim W. , Klein F. J. , Klimenko V. , Kripko A. , Kubarovsky V. , Lanza L. , Lenisa P. , Li X. , MacGregor I. J. D. , Marchand D. , Mascagna V. , Matamoros D. , Migliorati S. , Mokeev V. , Munoz Camacho C. , Nadel-Turonski P. , Neupane K. , Niccolai S. , Niculescu G. , Osipenko M. , Pandey P. , Paolone M. , Pappalardo L. L. , Paremuzyan R. , Pasyuk E. , Paul S. J. , Phelps W. , Pilleux N. , Pokhrel M. , Polcher Rafael S. , Prok Y. , Reed T. , Richards J. , Ripani M. , Ritchie B. G. , Ritman J. , Rosner G. , Salgado C. , Schadmand S. , Schmidt A. , Schumacher R. A. , Sharabian Y. G. , Shirokov E. V. , Shrestha U. , Sokhan D. , Sparveris N. , Spreafico M. , Stepanyan S. , Strakovsky I. I. , Strauch S. , Tan J. A. , Tenorio M. , Trotta N. , Tyson R. , Ungaro M. , Venturelli L. , Voskanyan H. , Voutier E. , Watts D. P. , Wei X. , Williams R. , Xu L. , Zachariou N. , Zhao Z. W. , Zurek M. ,   The CLAS Collaboration
Published: February 2025

Measurements of the polarization observables Σ,P,T,Ox,Oz for the reaction γ⃗p→KS0Σ+ using a linearly polarized photon beam of energy 1.1 to 2.1 GeV are reported.
The measured data provide information on a channel that has not been studied extensively, but is required for a full coupled-channel analysis in the nucleon resonance region.
Observables have been simultaneously extracted using likelihood sampling with a Markov-Chain Monte Carlo process.
Angular distributions in bins of photon energy Eγ are produced for each polarization observable. T,Ox, and Oz are first time measurements of these observables in this reaction. The extraction of Σ extends the energy range beyond a previous measurement. The measurement of P, the recoil polarization, is consistent with previous measurements.
The measured data are shown to be significant enough to affect the estimation of the nucleon resonance parameters when fitted within a coupled-channels model.Published by the American Physical Society2025
Strong interaction induced dimensional crossover in one-dimensional quantum gas
Authors: Zhang Zhongchi , Zhao Zihan , Wang Huaichuan , Deng Ken , Liu Yuqi , Chen Wenlan , Hu Jiazhong 
Published: February 2025

We generated a one-dimensional quantum gas confined in an elongated optical dipole trap instead of 2D optical lattices. The sample, comprising thousands of atoms, spans several hundred micrometers and allows for independent control of temperature and chemical potential using Feshbach resonance. This allows us to directly observe and investigate the spatial distribution and associated excitation of 1D quantum gas without any ensemble averaging. In this system, we observed that the dimension of 1D gas will be popped up into 3D due to strong interaction without changing any trapping confinement. During the dimensional crossover, we found that increasing the scattering length leads to the failure of 1D theories, including 1D mean field, Yang-Yang equation, and 1D hydrodynamics. Specifically, the modified Yang-Yang equation effectively describes this 1D system at temperatures beyond the 1D threshold, but it does not account for the effects of stronger interactions. Meanwhile, we observe two possible quantized plateaus of breathing-mode oscillation frequencies predicted by 1D and 3D hydrodynamics, corresponding to weak and strong interactions, respectively. There is also a universal crossover connecting two different regimes where both hydrodynamics fail.

Published by the American Physical Society
2025
Toward comprehensive understanding of m-wallet adoption: the significance of user perceptions and financial autonomy
Authors: Hidayat-ur-Rehman Imdadullah , Hossain Md. Nahin , Bhuiyan Abul Bashar , Zulkifli Norhayah 
Published: February 2025

Purpose
Recognizing the transformative impact of financial technology (Fintech) and mobile wallets (m-wallets) on modern financial practices, this study aims to explore the complex dynamics of m-wallet adoption in Bangladesh, focusing on user perceptions and financial autonomy. By integrating self-determination theory (SDT) and diffusion of innovation (DOI) theory, the authors propose a model incorporating constructs such as perceived security (PS), perceived trust (PT), compatibility, ease of use (EOU), perceived financial autonomy (PFA), perceived financial competence (PFC), relative advantage (RA) and intention-to-use (IU).

Design/methodology/approach
This study used a survey-based methodology to gather data from m-wallets users in Bangladesh. In this survey, 445 individuals participated; 393 of those were deemed legitimate and were chosen for study. Partial least squares-structural equation modeling was used in the investigation. This method made sure that the relationships between the proposed model’s constructs were thoroughly examined.

Findings
The research findings confirm that PFC significantly impacts the IU m-wallets and PFA. PFA also positively influences the IU. PS impacts the IU and PT, which further affects the IU. EOU influences RA and the IU. The study highlights the importance of PFC, PS, PT and EOU in driving the adoption of m-wallets.

Originality/value
This study integrates SDT and DOI theory to explore m-wallet adoption. It highlights financial competence and autonomy as key factors, offering insights and practical strategies for Fintech stakeholders in emerging markets to enhance adoption through financial literacy, security measures and user-friendly designs.