bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences
Authors: Monsen Robert C , DeLeeuw Lynn W , Dean William L , Gray Robert D , Chakravarthy Srinivas , Hopkins Jesse B , Chaires Jonathan B , Trent John O 
Published: March 2022

Abstract
We report on higher-order G-quadruplex structures adopted by long promoter sequences obtained by an iterative integrated structural biology approach. Our approach uses quantitative biophysical tools (analytical ultracentrifugation, small-angle X-ray scattering, and circular dichroism spectroscopy) combined with modeling and molecular dynamics simulations, to derive self-consistent structural models. The formal resolution of our approach is 18 angstroms, but in some cases structural features of only a few nucleotides can be discerned. We report here five structures of long (34–70 nt) wild-type sequences selected from three cancer-related promoters: c-Myc, c-Kit and k-Ras. Each sequence studied has a unique structure. Three sequences form structures with two contiguous, stacked, G-quadruplex units. One longer sequence from c-Myc forms a structure with three contiguous stacked quadruplexes. A longer c-Kit sequence forms a quadruplex-hairpin structure. Each structure exhibits interfacial regions between stacked quadruplexes or novel loop geometries that are possible druggable targets. We also report methodological advances in our integrated structural biology approach, which now includes quantitative CD for counting stacked G-tetrads, DNaseI cleavage for hairpin detection and SAXS model refinement. Our results suggest that higher-order quadruplex assemblies may be a common feature within the genome, rather than simple single quadruplex structures.
ASTE Band 10 (787–950 GHz) heterodyne receiver: System description, commissioning, and science verification
Authors: Asayama Shin’ichiro , Kamazaki Takeshi , Ishii Shun , Ito Tetsuya , Fujii Yasunori , Iono Daisuke , Gonzalez Alvaro , Inata Motoko , Sakamoto Seiichi 
Published: March 2022

Abstract
We report the system description and results of the commissioning and science verification (CSV) of the Band 10 (787–950 GHz) heterodyne receiver for ASTE (Atacama Submillimeter Telescope Experiment), a 10 m submillimeter telescope at the ALMA (Atacama Large Millimeter/submillimeter Array) site in Chile. The new ASTE Band 10 receiver cartridge was refurbished from a prototype ALMA Band 10 receiver with SIS mixers employing high critical current density junctions. We installed the new receiver on ASTE and carried out its CSV. The best double-sideband (DSB) system noise temperature achieved on ASTE toward the zenith was ∼1500 K (PWV ∼ 0.5  mm). As a part of CSV activities, we performed science verification observations to demonstrate ASTE Band 10 capabilities to the community. Wide-field images were successfully obtained toward the well-studied OMC-1 region with both [C i](3P2–3P1) and 12CO(J = 7–6) lines. In addition, we partially carried out observations toward a super star cluster RCW 38, [C i](3P1–3P0), images of which were already obtained by ASTE. We demonstrated the capability of ASTE Band 10 observations toward bright and extended objects like giant molecular clouds.
Appraising the need for audiological assessment before autism spectrum disorder referral
Authors: Mishaal Ram A , Weikum Whitney M , Brooks Beth , Derry Karen , Lanphear Nancy E 
Published: March 2022

Abstract

Objectives
Mandatory audiological testing before autism spectrum disorder (ASD) assessment is common practice. Hearing impairment (HI) in the general paediatric population is estimated at 3%; however, hearing impairment prevalence among children with ASD is poorly established. Our objective was to determine which children referred for ASD assessment require preliminary audiological assessment.

Methods
Retrospective chart review of children (n=4,173; 0 to 19 years) referred to British Columbia’s Autism Assessment Network (2010 to 2014). We analyzed HI rate, risk factors, and timing of HI diagnosis relative to ASD referral.

Results
ASD was diagnosed in 53.4%. HI rates among ASD referrals was 3.3% and not significantly higher in children with ASD (ASD+; 3.5%) versus No-ASD (3.0%). No significant differences in HI severity or type were found, but more ASD+ females (5.5%) than ASD+ males (3.1%) had HI (P&lt;0.05). Six HI risk factors were significant (problems with intellect, language, vision/eye, ear, genetic abnormalities, and prematurity) and HI was associated with more risk factors (P&lt;0.01). Only 12 children (8.9%) were diagnosed with HI after ASD referral; all males 6 years or younger and only one had no risk factors. ASD+ children with HI were older at ASD referral than No-ASD (P&lt;0.05).

Conclusions
Children with ASD have similar hearing impairment rates to those without ASD. HI may delay referral for ASD assessment. As most children were diagnosed with HI before ASD referral or had at least one risk factor, we suggest that routine testing for HI among ASD referrals should only be required for children with risk factors.
The Uptake and Effectiveness of a Multidisciplinary Online Program for Managing Chronic Pain before and During the COVID-19 Pandemic
Authors: Shiner Christine T , Gardner Tania , Haskelberg Hila , Li Ian , Faux Steven G , Millard Michael , Mahoney Alison E J 
Published: March 2022

Abstract

Objectives
Societal and health system pressures associated with the coronavirus disease 2019 (COVID-19) pandemic exacerbated the burden of chronic pain and limited access to pain management services for many. Online multidisciplinary pain programs offer an effective and scalable treatment option, but have not been evaluated within the context of COVID-19. This study aimed to investigate the uptake and effectiveness of the Reboot Online chronic pain program before and during the first year of the COVID-19 pandemic.

Methods
Retrospective cohort analyses were conducted on routine service users of the Reboot Online program, comparing those who commenced the program during the COVID-19 pandemic (March 2020–March 2021), to those prior to the pandemic (April 2017–March 2020). Outcomes included the number of course registrations; commencements; completion rates; and measures of pain severity, interference, self-efficacy, pain-related disability, and distress.

Results
Data from 2,585 course users were included (n = 1138 pre-COVID-19 and n = 1,447 during-COVID-19). There was a 287% increase in monthly course registrations during COVID-19, relative to previously. Users were younger, and more likely to reside in a metropolitan area during COVID-19, but initial symptom severity was comparable. Course adherence and effectiveness were similar before and during COVID-19, with moderate effect size improvements in clinical outcomes post-treatment (g = 0.23–0.55).

Discussion
Uptake of an online chronic pain management program substantially increased during the COVID-19 pandemic. Program adherence and effectiveness were similar pre- and during-COVID. These findings support the effectiveness and scalability of online chronic pain management programs to meet increasing demand.
TEMPORAL VARIATION IN RADIOCESIUM CONCENTRATIONS IN WATERS OF THE NATSUI AND SAME RIVERS, SOUTH FUKUSHIMA PREFECTURE, JAPAN FROM 2011 TO 2020
Authors: Nagao Seiya , Kanamori Masaki , Shimamura Akie , Morokado Toshiki , Putra D I P , Fujita Tomoki , Tomihara Seiichi , Ochiai Shinya 
Published: March 2022

Abstract
In order to determine the long-term impacts of radiocesium contamination on ecosystems in watersheds and coastal areas following the Fukushima Daiichi Nuclear Power Plant accident in March 2011, it was important to monitor the transport behavior of radiocesium from an early stage. In this study, conducted from July 2011 to October 2020, we carried out field research along the Natsui and Same rivers running through watersheds in the south of Fukushima Prefecture, Japan, in which there had been a relatively low accumulation of radiocesium. We found that under normal flow conditions, the total (dissolved + particulate phase) activity of 137Cs decreased with increasing time following the accident. However, the water samples collected after rain events showed higher activity of up to 895 mBq l−1 with a higher percentage (&gt;92% of total) of the particulate phase. These findings indicate that radiocesium deposited on the ground surface is predominantly transported in the particulate phase from watersheds to rivers via precipitation. The decontamination process, which was performed in the farmland during December 2014 to March 2015 and in forest during February 2013 to April 2014, was small effects of the transport of 137Cs. Under normal flow conditions, total 137Cs activity was largely determined by the suspended solids (SS) concentration and/or 137Cs concentration in the SS.
Longitudinal change in neural response to vocal emotion in adolescence
Authors: Morningstar Michele , Mattson Whitney I , Nelson Eric E 
Published: March 2022

Abstract
Adolescence is associated with maturation of function within neural networks supporting the processing of social information. Previous longitudinal studies have established developmental influences on youth’s neural response to facial displays of emotion. Given the increasing recognition of the importance of non-facial cues to social communication, we build on existing work by examining longitudinal change in neural response to vocal expressions of emotion in 8- to 19-year-old youth. Participants completed a vocal emotion recognition task at two timepoints (1 year apart) while undergoing functional magnetic resonance imaging. The right inferior frontal gyrus, right dorsal striatum and right precentral gyrus showed decreases in activation to emotional voices across timepoints, which may reflect focalization of response in these areas. Activation in the dorsomedial prefrontal cortex was positively associated with age but was stable across timepoints. In addition, the slope of change across visits varied as a function of participants’ age in the right temporo-parietal junction (TPJ): this pattern of activation across timepoints and age may reflect ongoing specialization of function across childhood and adolescence. Decreased activation in the striatum and TPJ across timepoints was associated with better emotion recognition accuracy. Findings suggest that specialization of function in social cognitive networks may support the growth of vocal emotion recognition skills across adolescence.
Making the Railways Pay: The Redevelopment of Euston Station, Labour and Conservative Visions of Public Sector Property Speculation in the 1960s and 1970s
Authors: Harrison Ewan 
Published: March 2022

Abstract
This article examines the redevelopment of Euston Station forecourt as a speculative development of offices designed by the prolific post-war commercial architectural practice R. Seifert & Partners from c.1970 to 1979. The article offers a reading of the development as a piece of state-encouraged, public-sector led property development. It uncovers the encouragement given by Harold Wilson’s Labour government to for-profit property developments on nationalized land and the simultaneous control of private sector office development by the same government. It reveals a consistent labourite vision of public sector property development.
Diversity of schizomids (Arachnida: Schizomida) revealed by new fossil genera and species from mid-Cretaceous Burmese amber with implications for a Gondwanan origin of the Burma Terrane
Authors: De Francesco Magnussen Ilian , Müller Sandro P , Hammel Jörg U , Kotthoff Ulrich , Harms Danilo 
Published: March 2022

Abstract
Short-tailed whipscorpions are a small arachnid order with 379 described species from (sub)tropical regions of the world. The order has a fragmentary fossil record but has left a rich, albeit hitherto undocumented, fossil legacy in mid-Cretaceous amber from Myanmar, also called Burmese amber or Burmite. Here, we review a large series of schizomids from this type of amber and propose four new genera for seven new species. In addition, we include a short revision of all schizomid genera based on 14 morphological characters. Although none of the fossils can be assigned to Recent genera, they resemble the modern fauna in general morphology and highlight relative morphological stasis in this arachnid order. The fossils show a general trend towards eye reduction, but some of the specimens retain corneate eyes. Comparative data suggest affinities of the fossils with the Recent Old World fauna rather than American relatives. Furthermore, the fossils provide evidence for a Gondwanan origin of the Burma Terrane. Overall, the data suggest a high diversity at both species and genus levels for Burmese amber schizomids. We discuss potential reasons for this richness that will probably necessitate the description of additional genera and species in future studies.
Evaluating Nurses’ Knowledge of Pressure Injury Prevention: A Descriptive Study
Authors: Dirgar Ezgi , Tosun Betül , Dokumuş Hakan , Bülbül Tuba 
Published: March 2022

ABSTRACT

OBJECTIVE
To evaluate nurses’ knowledge on pressure injury (PI) prevention.

METHODS
Four hundred six nurses in Turkey participated in this descriptive study between January and February 2020. Participants completed an information form and the Pressure Ulcer Prevention Knowledge Assessment Instrument (PUPKAI). The PUPKAI contains 26 items across six themes: (1) development, (2) classification and observation, (3) risk assessment, (4) nutrition, (5) interventions to reduce the amount of pressure/rupture, and (6) interventions to reduce the duration of pressure/rupture.

RESULTS
The mean age of the nurses was 26.93 ± 6.72 years. The mean total PUPKAI score was 11.80 ± 3.28. Almost all participants (91.6%) had insufficient knowledge on PI prevention. Male nurses scored higher on themes 1 and 2 than female nurses (P = .046, P = .029, respectively). Nurses with a postgraduate degree scored higher in total and on themes 2, 3, 5, and 6 (P = .001, P = .006, P = .011, P = .044, P = .029, respectively), and nurses with more than 10 years of service scored higher in total and on themes 4, 5, and 6 (P = .001, P = .002, P = .001, P = .026, respectively).

CONCLUSIONS
The nurses who participated in this study do not have enough knowledge on PI prevention.
The Three-Dimensional Structure of Porcine Bladder Scaffolds Alters the Biology of Murine Diabetic Wound Healing
Authors: Liu Qinxin , Wu Mengfan , Karvar Mehran , Aoki Shimpo , Endo Yori , Hamaguchi Ryoko , Ma Chenhao , Matar Dany Y. , Orgill Dennis P. , Panayi Adriana C. 
Published: March 2022

ABSTRACT

OBJECTIVE
To determine if the various three-dimensional structures of bioscaffolds affect wound healing by investigating the efficacy of different porcine-derived urinary bladder matrix (UBM) structures in treating murine diabetic wound healing.

METHODS
The authors studied three different UBM structures: particulate (pUBM), one-layer freeze-dried sheet (fdUBM), and three-layer laminated sheet (lmUBM). Scanning electron microscopy images of the structures were used to calculate a wound-exposed surface-area-to-volume ratio. A 1.0 × 1.0-cm full-thickness dorsal wound was excised on 90 db/db mice. Mice were either untreated (blank, n = 15), treated with one UBM structure (pUBM, n = 15; fdUBM, n = 15; lmUBM, n = 15), or treated with a combination of either the one- or three-layer sheet over the particulate matrix (fdUBM + pUBM, n = 15; lmUBM + pUBM, n = 15). The authors obtained macroscopic images of the wounds and harvested tissues for analyses at multiple time points.

RESULTS
The surface area available to interact with the wound was highest in the pUBM group and lowest in the lmUBM group. Greater wound bed thickness was noted in the fdUBM, fdUBM + pUBM, and lmUBM groups compared with the blank group. Cellular proliferation was significantly higher in the fdUBM and fdUBM + pUBM groups than in the blank group. The lmUBM + pUBM group had the highest collagen deposition. The pUBM group induced significantly higher leukocyte infiltration compared with the lmUBM, lmUBM + pUBM, and blank groups. Microvessel density was highest in the fdUBM + pUBM group. Significant differences in the wound closure rate were noted between the blank group and the fdUBM and fdUBM + pUBM groups.

CONCLUSIONS
Assessment of the three UBM bioscaffold structures highlighted differences in the wound-exposed surface area. Variations in wound healing effects, including collagen deposition, cellular proliferation, and angiogenesis, were identified, with combinations of the structures displaying synergistic effects. This study serves as a platform for future scaffold design and offers promising evidence of the benefits of combining various structures of scaffolds.
Quality of Life and Its Influencing Factors in Chinese Patients with Chronic Wounds
Authors: Li Siqing , Xie Minyi , Luo Wenxin , Zhou Qing , Li Caifei , Liu Yuan , Hu Ailing 
Published: March 2022

ABSTRACT

OBJECTIVE
To explore the factors influencing quality of life in patients with chronic wounds.

METHODS
A total of 138 patients with chronic wounds were included and completed the Chinese version of the Cardiff Wound Impact Schedule, the Hospital Anxiety and Depression Scale, and the Medical Coping Modes Questionnaire after researchers assessed their wound severity.

RESULTS
The mean scores of physical symptoms and everyday living, social life, and well-being on the Chinese version of the Cardiff Wound Impact Schedule were 55.65 ± 15.82, 52.69 ± 16.46, and 40.75 ± 13.82, respectively. Multiple regression analysis revealed that depression, coping style, and education level were the primary factors, explaining 51.7% of the variance in physical symptoms and everyday living. Depression and coping style explained 49.6% of the variance in social life. Anxiety and primary disease explained 36.2% of the variance in well-being.

CONCLUSIONS
The factors influencing quality of life for these Chinese patients with chronic wounds included education level, primary disease, anxiety, depression, and coping style. Speaking with patients about living with a chronic wound and their potential for healing, understanding their fears about significant changes to their life, and encouraging them to accept such changes can be important for patients and their recovery.
Dietary acid load and the risk of cancer: a systematic review and dose-response meta-analysis of observational studies
Authors: Bahrami Alireza , Khalesi Saman , Ghafouri-Taleghani Fatemeh , Alibeyk Sepide , Hajigholam- Saryazdi Maryam , Haghighi Shirin , Hejazi Ehsan 
Published: March 2022

Aims
Recent evidence suggests the link between adherence to an acidogenic diet and the risk of some types of cancers, such as colorectal and breast cancers. This systematic review and meta-analysis aimed to clarify the association between dietary acid load and cancer risk.

Data search and synthesis
Online databases (PubMed, Scopus, EMBASE, Scholar Google and ISI web of sciences) were searched between January 1990 and May 2021. The risk ratio (RR) was extracted from eligible studies and random-effects meta-analysis was performed to calculate pooled RR of studies. Nine studies (three cohorts, six case–control) were included. Higher dietary acid load scores [including potential renal acid load (PRAL) and net endogenous acid production (NEAP)] were associated with the increased risk of cancer [RRPRAL, 1.77; 95% confidence interval (CI), 1.27–2.46; n = 8; RRNEAP, 1.58, 95% CI: 1.20–2.09, n = 7). Dose-response analysis suggested that a 20-score increase in dietary PRAL and NEAP was associated with 27 and 8% higher risk of cancer, respectively (RRPRAL, 1.27; 95% CI, 1.02–1.60; nonlinearity P = 0.12; RRNEAP, 1.08; 95% CI, 1.02–1.13, nonlinearity P = 0.06). A significant positive relationship between dietary PRAL and risk of cancer was only observed in the subgroup of women. Associations were significant in both men and women for dietary NEAP. Subgroup analyses based on cancer type were only possible for breast cancer. There was no significant association between dietary acid load (PRAL and NEAP) and breast cancer risk.

Conclusion
Our analysis showed that high adherence to an acidogenic diet is associated with an increased risk of cancer. The protocol for this meta-analysis was registered in PROSPERO registration no. CRD42019146460.
Radiological HEPA Filter 10-year Lifetime Evaluation in Research Facilities
Authors: Barnett J. Matthew , Bliss Mary , Schrank Kobe R. , Edwards Hunter Z. , Brown David M. , McDonald Kent M. , Cooley Scott K. 
Published: March 2022

Abstract
High-efficiency particulate air (HEPA) filters are widely employed by nuclear facilities to remove radiological particulate matter from their effluent exhaust streams. The purpose of this study is to evaluate the relationships between the 10-y HEPA filter lifetime deployment and its other performance indicators. This 10-y-long endeavor to collect and analyze data regarding the service life of HEPA filters at the Pacific Northwest National Laboratory began in 2010. A set of HEPA filters was selected, and the filters have been surveyed and analyzed at least annually to verify compliance with permit conditions. The study suggests the frequency of filter replacement should be based on the actual operational requirements, such as fume hood face velocity and/or efficiency test results, instead of on the prescribed filter “age limit” of 10 y from the date of manufacture (e.g., birth date) when operating under dry conditions. The study has now been completed, and over the past decade, all the HEPA filters have been replaced due to either technical issues as listed in this report or the previously recommended filter “age limit” of 10 y as prescribed by the oversight bodies. Experimentally determined failure rates are also determined from the data set and can be used to estimate the chances of HEPA filters surviving 15, 20, or even 30 y.
Telehealth
Authors: Hills Zoi P. , Cruz Rachel , Neufeld Jonathan , DeVany Mary 
Published: March 2022

ABSTRACT
Modern telehealth has existed since the second half of the 20th century and has recently been gaining wider attention because of the COVID-19 pandemic. This article provides an overview of historical milestones in the development of telehealth, including various ways that individuals and organizations have innovated in the field, and regulatory milestones that have aided the proliferation of telehealth in the United States. The second half of the article provides a broad overview of the pros and cons of telehealth, focusing on both individual- and system-level effects. Given the increasing interest in telehealth—among consumers, healthcare providers, and mainstream technology giants—telehealth is likely to continue to be an important tool for healthcare delivery for the foreseeable future.