bluuebunny/crossref_metadata_2025_split huggingface dataset:
NADP+ supply adjusts the synthesis of photosystem I in Arabidopsis chloroplasts
Authors: Ji Daili , Li Qiuxin , Guo Yinjie , An Wenjing , Manavski Nikolay , Meurer Jörg , Chi Wei 
Published: April 2022

Abstract
In oxygenic photosynthesis, NADP+ acts as the final acceptor of the photosynthetic electron transport chain and receives electrons via the thylakoid membrane complex photosystem I (PSI) to synthesize NAPDH by the enzyme ferredoxin:NADP+ oxidoreductase. The NADP+/NADPH redox couple is essential for cellular metabolism and redox homeostasis. However, how the homeostasis of these two dinucleotides is integrated into chloroplast biogenesis remains largely unknown. Here, we demonstrate the important role of NADP+ supply for the biogenesis of PSI by examining the nad kinase 2 (nadk2) mutant in Arabidopsis (Arabidopsis thaliana), which demonstrates disrupted synthesis of NADP+ from NAD+ in chloroplasts. Although the nadk2 mutant is highly sensitive to light, the reaction center of photosystem II (PSII) is only mildly and likely only secondarily affected compared to the wild-type. Our studies revealed that the primary limitation of photosynthetic electron transport, even at low light intensities, occurs at PSI rather than at PSII in the nadk2 mutant. Remarkably, this primarily impairs the de novo synthesis of the two PSI core subunits PsaA and PsaB, leading to the deficiency of the PSI complex in the nadk2 mutant. This study reveals an unexpected molecular link between NADK activity and mRNA translation of psaA/B in chloroplasts that may mediate a feedback mechanism to adjust de novo biosynthesis of the PSI complex in response to a variable NADPH demand. This adjustment may be important to protect PSI from photoinhibition under conditions that favor acceptor side limitation.
Inhibiting thyroid activation in aged human explants prevents mechanical induced detrimental signalling by mitigating metabolic processes
Authors: Houtman Evelyn , Tuerlings Margo , Suchiman H Eka D , Lakenberg Nico , Cornelis Frederique M F , Mei Hailiang , Broekhuis Demiën , Nelissen Rob G H H , Coutinho de Almeida Rodrigo , Ramos Yolande F M , Lories Rik J , Cruz Luis J , Meulenbelt Ingrid 
Published: April 2022

Abstract

Objectives
To investigate whether the deiodinase inhibitor iopanoic acid (IOP) has chondroprotective properties, a mechanical stress induced model of human aged explants was used to test both repeated dosing and slow release of IOP.

Methods
Human osteochondral explants subjected to injurious mechanical stress (65%MS) were treated with IOP or IOP encapsulated in poly lactic-co-glycolic acid–polyethylene glycol nanoparticles (NP-IOP). Changes to cartilage integrity and signalling were determined by Mankin scoring of histology, sulphated glycosaminoglycan (sGAG) release and expression levels of catabolic, anabolic and hypertrophic markers. Subsequently, on a subgroup of samples, RNA sequencing was performed on 65%MS (n = 14) and 65%MS+IOP (n = 7) treated cartilage to identify IOP’s mode of action.

Results
Damage from injurious mechanical stress was confirmed by increased cartilage surface damage in the Mankin score, increased sGAG release, and consistent upregulation of catabolic markers and downregulation of anabolic markers. IOP and, though less effective, NP-IOP treatment, reduced MMP13 and increased COL2A1 expression. In line with this, IOP and NP-IOP reduced cartilage surface damage induced by 65%MS, while only IOP reduced sGAG release from explants subjected to 65%MS. Lastly, differential expression analysis identified 12 genes in IOP’s mode of action to be mainly involved in reducing metabolic processes (INSIG1, DHCR7, FADS1 and ACAT2) and proliferation and differentiation (CTGF, BMP5 and FOXM1).

Conclusion
Treatment with the deiodinase inhibitor IOP reduced detrimental changes of injurious mechanical stress. In addition, we identified that its mode of action was likely on metabolic processes, cell proliferation and differentiation.
Longitudinal association of infrapatellar fat pad signal intensity alteration with biochemical biomarkers in knee osteoarthritis
Authors: Cen Han , Yan Qingran , Han Weiyu , Meng Tao , Chen Zhongshan , Ruan Guangfeng , Wang Tian , Pan Feng , Chen Di , Kraus Virginia Byers , Hunter David J , Ding Changhai 
Published: April 2022

Abstract

Objective
To explore the longitudinal association of quantitative infrapatellar fat pad (IPFP) signal intensity alteration with OA-related biomarkers.

Methods
Eighteen OA-related biochemical biomarkers of 600 knee OA participants in the Foundation for the National Institutes of Health OA Biomarkers Consortium (FNIH) study were extracted. The quantitative IPFP signal intensity measures were acquired based on magnetic resonance imaging, including mean value [Mean (IPFP)] and standard deviation [sDev (IPFP)] of the whole IPFP signal intensity, median value [Median (H)] and upper quartile value [UQ (H)] of high signal intensity, the ratio of volume of high signal intensity to volume of whole IPFP signal intensity [Percentage (H)] and Clustering factor (H). The linear mixed-effect model was applied to determine the longitudinal associations between IPFP signal intensity alteration and biochemical biomarkers over 2 years.

Results
All IPFP measures except for Clustering factor (H) were positively associated with urine collagenase-cleaved type II collagen neoepitope (uC2C), urine C-terminal cross-linked telopeptide of type II collagen (uCTX-II), urine C-terminal cross-linked telopeptide of type I collagen-α (uCTX-Iα) and urine N-terminal cross-linked telopeptide of type I collagen (uNTX-I). Mean (IPFP), Median (H) and Percentage (H) were positively associated with the nitrated form of an epitope located in the triple helix of type II collagen (Coll2-1 NO2). Mean (IPFP), Median (H) and UQ (H) were positively associated with sCTX-I and uCTX-Iβ. Positive associations between sDev (IPFP), Percentage (H) and serum hyaluronic acid (sHA) were found.

Conclusion
Our results suggest a role of IPFP signal intensity alteration in joint tissue remodelling on a molecular level.
Microglia-independent peripheral neuropathic pain in male and female mice
Authors: Tu YuShan , Muley Milind M. , Beggs Simon , Salter Michael W. 
Published: April 2022

Abstract
The dominant view in the field of pain is that peripheral neuropathic pain is driven by microglia in the somatosensory processing region of the spinal dorsal horn. Here, to the contrary, we discovered a form of neuropathic pain that is independent of microglia. Mice in which the nucleus pulposus (NP) of the intervertebral disc was apposed to the sciatic nerve developed a constellation of neuropathic pain behaviours: hypersensitivity to mechanical, cold, and heat stimuli. However, NP application caused no activation of spinal microglia nor was pain hypersensitivity reversed by microglial inhibition. Rather, NP-induced pain hypersensitivity was dependent on cells within the NP which recruited macrophages to the adjacent nerve. Eliminating macrophages systemically or locally prevented NP-induced pain hypersensitivity. Pain hypersensitivity was also prevented by genetically disrupting the neurotrophin brain-derived neurotrophic factor selectively in macrophages. Moreover, the behavioural phenotypes as well as the molecular mechanisms of NP-induced pain hypersensitivity were not different between males and females. Our findings reveal a previously unappreciated mechanism for by which a discrete peripheral nerve lesion may produce pain hypersensitivity, which may help to explain the limited success of microglial inhibitors on neuropathic pain in human clinical trials.
Cognitive functional therapy compared with core exercise and manual therapy in patients with chronic low back pain: randomised controlled trial
Authors: Castro Julia , Correia Luis , Donato Bruno de Sousa , Arruda Bruno , Agulhari Felipe , Pellegrini Marina J. , Belache Fabiana T.C. , de Souza Cíntia P. , Fernandez Jessica , Nogueira Leandro A.C. , Reis Felipe J.J. , Ferreira Arthur de Sá , Meziat-Filho Ney 
Published: April 2022

Abstract
Cognitive functional therapy (CFT) is a physiotherapy-led intervention that has evolved from an integration of foundational behavioral psychology and neuroscience within the physiotherapist practice directed at the multidimensional nature of chronic low back pain (CLBP). The current evidence about the comparative effectiveness of CFT for CLBP is still scarce. We aimed to investigate whether CFT is more effective than core training exercise and manual therapy (CORE-MT) in pain and disability in patients with CLBP. A total of 148 adults with CLBP were randomly assigned to receive 5 one-hour individualized sessions of either CFT (n = 74) or CORE-MT (n = 74) within a period of 8 weeks. Primary outcomes were pain intensity (numeric pain rating scale, 0-10) and disability (Oswestry Disability Index, 0-100) at 8 weeks. Patients were assessed preintervention, at 8 weeks and 6 and 12 months after the first treatment session. Altogether, 97.3% (n = 72) of patients in each intervention group completed the 8 weeks of the trial. Cognitive functional therapy was more effective than CORE-MT in disability at 8 weeks (MD = −4.75; 95% CI −8.38 to −1.11; P = 0.011, effect size= 0.55) but not in pain intensity (MD = −0.04; 95% CI −0.79 to 0.71; P = 0.916). Treatment with CFT reduced disability, but the difference was not clinically important compared with CORE-MT postintervention (short term) in patients with CLBP. There was no difference in pain intensity between interventions, and the treatment effect was not maintained in the mid-term and long-term follow-ups.
Innovative Assessments for Retention of Sexual Assault Nurse Examiners
Authors: Hite Amy , Overstreet Wendy , Giefer Cheryl , Belcher Greg 
Published: April 2022

ABSTRACT
The Southeast Kansas (SEK) comprehensive rural Sexual Assault Nurse Examiners (SANE) program was developed as part of an effort to increase the availability of SANE services nationwide. The primary goal of the program is to provide education to assist with training and certification of registered nurses and advanced practice registered nurses in SEK to conduct sexual assault forensic examinations and become certified. This will provide better physical and mental health care for survivors and better evidence collection and higher prosecution rates in a rural, medically underserved area. The project established a rural academic–practice–community partnership to provide longitudinal immersive SANE clinical training. The success of the project in SEK will be impacted by the retention of SANEs; therefore, the integration of the Professional Quality of Life (ProQOL) self-assessment was seen as an important component of the grant implementation. ProQOL determines compassion satisfaction, burnout, and secondary traumatic stress. The data collected from SANEs on their self-assessments with the ProQOL lead to early identification of SANEs experiencing negative effects of forensic nursing and the ability to intervene for decreased attrition.
Prescription for Success
Authors: Barbanel-Yuni Julie , Darcy Jennifer 
Published: April 2022

Introduction
It was noted that caregivers of pediatric surgical patients are often not able to speak to the correct disposal of leftover narcotic pain medication. The purpose of this process improvement project was to increase the knowledge and use of activated-carbon-based deactivation kits during the discharge process to increase safe medication disposal.

Methods
A team was formed to increase safe disposal. The team developed a plan for the pharmacy to provide quick facts when dispensing narcotic pain medication, giving instructions to the parents about how to obtain an activated-carbon-based deactivation kit if they had unused narcotic medication. In addition, the dangers of incorrect disposal of narcotics were included. The nurses provided verbal education to the parents on the safe disposal at discharge to home. Furthermore, parents were reminded of the opportunity to utilize an activated-carbon-based deactivation kit provided from the hospital pharmacy to dispose of the narcotics during postsurgical follow-up call.

Results
The hospital pharmacy saw a sixfold increase in the dispensing of activated-carbon-based deactivation kits since the education was initiated. Caregivers noted a 68% increase in reporting of proper disposal of narcotics.

Discussion
Implementation of a standardized educational practice for the families of patients being discharged with narcotic medication resulted in an increased awareness of safe disposal of narcotics and an increase in dispensing of activated-carbon-based deactivation kits. Ultimately, this resulted in the families having a greater confidence in caring for their children at discharge as reported on patient satisfaction surveys.
High Expression of Circular RNA–Mitochondrial tRNA Translation Optimization 1 Assists the Diagnosis of High-Risk Human Papillomavirus Infection in Cervical Cancer
Authors: Cheng Xiyun , Shen Changmei , Liao Zhenrong 
Published: April 2022

Objective
Persistence of high-risk human papillomavirus (HR-HPV) infection is a paramount determinant in cervical cancer (CC) development. Circular RNAs have the potential to be promising biomarkers for various cancers. This study explored circular RNA–mitochondrial tRNA translation optimization 1 (circMTO1) expression in the serum of CC patients and its clinical value in diagnosing CC and predicting HR-HPV infection.

Materials and Methods
In total, 125 CC patients (including 78 cases with HR-HPV) were enrolled, with another 76 healthy people as controls. Serum circMTO1 and miR-199a expressions were detected by reverse transcription–quantitative polymerase chain reaction, and the diagnostic efficacy of circMTO1 for CC and HR-HPV infection was analyzed by the receiver operating characteristic curve. According to the median of serum circMTO1 expression, CC patients were assigned into circMTO1 low/high expression groups to analyze the correlation between circMTO1 and clinical parameters using the Fisher and χ2 tests. Independent association of circMTO1 with HR-HPV infection in CC was evaluated via logistics multivariate regression analysis. Targeted relationship between miR-199a and circMTO1 was predicted by Starbase Web site and validated via dual-luciferase assay, with their correlation further assessed by Pearson analysis.

Results
Serum circMTO1 was increased in CC patients and prominently elevated in HR-HPV–positive CC patients, with a level greater than 1.485 assisting CC diagnosis and a level greater than 2.480 assisting HR-HPV–positive diagnosis. The circMTO1 was interrelated to clinical stage, tumor differentiation, lymph node metastasis, invasion depth, and independently linked with HR-HPV infection in CC. Serum miR-199a was downregulated in HR-HPV–positive CC patients and inversely correlated with circMTO1.

Conclusions
Serum circMTO1 is upregulated in HR-HPV–positive CC patients and has a diagnostic value for HR-HPV infection in CC.
Leisure Time Physical Activity in Children and Young People With Cerebral Palsy: A Population-Based Study
Authors: van der Linden Marietta L. , Wordie Sarah J. , Dufton Bethany K. , Jagadamma Kavi C. , Hunter Cathleen , Mercer Thomas H. , Gaston Mark S. , Robb James E. 
Published: April 2022

Purpose:
To describe leisure time physical activity in children and young people with cerebral palsy and identify barriers and facilitators to participation.

Methods:
Leisure time physical activity participation was derived from a national cerebral palsy register and associated factors were analyzed. Barriers and facilitators to participation were investigated through a survey.

Results:
Leisure time physical activity participation was recorded. Outcomes of participation decreased with increasing Gross Motor Function Classification System level. Leisure time physical activity “not in club” for 11- to 18-year-olds was significantly lower than for those aged 5 to 10 years for Gross Motor Function Classification System level II. The survey supported that disability and disliking help were common barriers and parental encouragement and enjoyment were common facilitators.

Conclusions:
Data from the register and survey provide insight into factors influencing leisure time physical activity participation in young people with cerebral palsy and how to increase.
Prevalence of Gastroesophageal Reflux Disease in Infants With Congenital Muscular Torticollis: A Prospective Cohort Study
Authors: Bess Laura K. , Costa Jessica , Nguygen Anh Thy H. , Amankwah Ernest , Wilsey Michael J. 
Published: April 2022

Purpose:
Clinical experience suggests that gastroesophageal reflux disease (GERD) occurs commonly in infants with congenital muscular torticollis (CMT). However, this is an understudied topic and prospective studies are absent. We determine the prevalence of GERD in infants with CMT, comparing clinical characteristics between CMT infants with and without GERD, and identifying infants with potentially undiagnosed GERD.

Methods:
A prospective cohort study of 155 infants with CMT younger than 12 months with and without GERD was evaluated by pediatric physical therapists.

Results:
GERD prevalence was 30.3%, including 6 (3.9%) infants with undiagnosed GERD. Demographic and clinical characteristics were similar in CMT infants with and without GERD.

Conclusions:
This is the first prospective cohort study determining the prevalence of GERD in infants referred for evaluation of CMT. Further prospective studies are needed to determine whether early intervention and treatment of GERD improves outcomes in infants with CMT (see Supplemental Digital Content 1, available at: http://links.lww.com/PPT/A369).
Study Protocol: Multimodal Longitudinal Assessment of Infant Brain Organization and Recovery in Perinatal Brain Injury
Authors: Saiote Catarina , Sutter Ellen , Xenopoulos-Oddsson Annette , Rao Raghavendra , Georgieff Michael , Rudser Kyle , Peyton Colleen , Dean Douglas , McAdams Ryan M. , Gillick Bernadette 
Published: April 2022

Purpose:
Perinatal brain injury is a primary cause of cerebral palsy, a condition resulting in lifelong motor impairment. Infancy is an important period of motor system development, including development of the corticospinal tract (CST), the primary pathway for cortical movement control. The interaction between perinatal stroke recovery, CST organization, and resultant motor outcome in infants is not well understood.

Methods:
Here, we present a protocol for multimodal longitudinal assessment of brain development and motor function following perinatal brain injury using transcranial magnetic stimulation and magnetic resonance imaging to noninvasively measure CST functional and structural integrity across multiple time points in infants 3 to 24 months of age. We will further assess the association between cortical excitability, integrity, and motor function.

Discussion:
This protocol will identify bioindicators of motor outcome and neuroplasticity and subsequently inform early detection, diagnosis, and intervention strategies for infants with perinatal stroke, brain bleeds, and related diagnoses.
Clinical Usefulness of 18F-FET PET in a Pediatric Patient With Suspected Demyelinating Disease
Authors: Kang Seo Young , Moon Byung Seok , Yoo Min Young , Yoon Hai-Jeon , Kim Bom Sahn 
Published: April 2022

Abstract
An 11-year-old boy who presented with headache and progressive right-sided weakness exhibited cortical swelling in the parafalcine area of both frontoparietal high convexity and splenium portion of corpus callosum on brain MRI. This suggested the possibility of encephalopathy, but required differential diagnosis from brain tumor. 18F-FET (O-(2-[18F]fluoroethyl)-l-tyrosine) PET/CT identified increased uptake along the parafalcine area of the frontoparietal lobes and the splenium portion of the corpus callosum. The relatively low target-to-background ratios were more indicative of inflammatory changes such as demyelinating disease. The patient recovered after empirical steroid and immunoglobulin treatment. Clinically, the patient was diagnosed with acute disseminated encephalomyelitis.
18F-SynVesT-1 PET in Focal Cortical Dysplasia Type II With Thickening Cortex
Authors: Tang Yongxiang , Yu Jie , Zhou Ming , Chen Chen , Hu Shuo 
Published: April 2022

Abstract
A case of 2-year-old girl with intractable seizures underwent 18F-FDG PET and MRI for seizure focus localization. MRI demonstrated cortical thickening and blurring of the gray-white matter interface in the right postcentral gyrus with focal hypometabolism in 18F-FDG PET. The patient subsequently was enrolled in clinical trial of 18F-SynVesT-1 PET study in epilepsy; a more restricted area of reduced 18F-SynVesT-1 uptake was noted in the thickened postcentral gyrus. The surgical limits of resection were defined based on ictal semiology, electroencephalography, and imagings. The patient is seizure-free after epilepsy surgery, with histopathology of focal cortical dysplasia type IIb.
Acute respiratory distress syndrome after SARS-CoV-2 infection on young adult population: international observational federated study based on electronic health records through the 4CE consortium
Authors: Moal Bertrand , Orieux Arthur , Ferté Thomas , Neuraz Antoine , Brat Gabriel A , Avillach Paul , Bonzel Clara-Lea , Cai Tianxi , Cho Kelly , Cossin Sébastien , Griffier Romain , Hanauer David A , Haverkamp Christian , Ho Yuk-Lam , Hong Chuan , Hutch Meghan R , Klann Jeffrey G , Le Trang T , Loh Ne Hooi Will , Luo Yuan , Makoudjou Adeline , Morris Michele , Mowery Danielle L , Olson Karen L , Patel Lav P , Samayamuthu Malarkodi J , Sanz Vidorreta Fernando J , Schriver Emily R , Schubert Petra , Verdy Guillaume , Visweswaran Shyam , Wang Xuan , Weber Griffin M , Xia Zongqi , Yuan William , Zhang Harrison G , Zöller Daniela , Kohane Isaac S ,   The Consortium for Clinical Characterization of COVID-19 by EHR (4CE), Boyer Alexandre , Jouhet Vianney 
Published: April 2022

AbstractPurposeIn young adults (18 to 49 years old), investigation of the acute respiratory distress syndrome (ARDS) after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been limited. We evaluated the risk factors and outcomes of ARDS following infection with SARS-CoV-2 in a young adult population.MethodsA retrospective cohort study was conducted between January 1st, 2020 and February 28th, 2021 using patient-level electronic health records (EHR), across 241 United States hospitals and 43 European hospitals participating in the Consortium for Clinical Characterization of COVID-19 by EHR (4CE). To identify the risk factors associated with ARDS, we compared young patients with and without ARDS through a federated analysis. We further compared the outcomes between young and old patients with ARDS.ResultsAmong the 75,377 hospitalized patients with positive SARS-CoV-2 PCR, 1001 young adults presented with ARDS ( 7.8% of young hospitalized adults). Their mortality rate at 90 days was 16.2% and they presented with a similar complication rate for infection than older adults with ARDS. Peptic ulcer disease, paralysis, obesity, congestive heart failure, valvular disease, diabetes, chronic pulmonary disease and liver disease were associated with a higher risk of ARDS. We described a high prevalence of obesity (53%), hypertension (38%-although not significantly associated with ARDS), and diabetes (32%).ConclusionTrough an innovative method, a large international cohort study of young adults developing ARDS after SARS-CoV-2 infection has been gather. It demonstrated the poor outcomes of this population and associated risk factor.