bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial hTERT rewires glucose metabolism to confer cancer chemoresistance by binding to MT-ND1
Authors: Li Xinzhe , Chang Xing , Hu Weichao , Zhao Yongbing , Xu Zhengguo , Gao Mengyuan , Ouyang Qin , Zhang Shengwei , Tang Qingyun , He Junjian , Wang Liting , Ling Xianlong , Yang Shiming , Hu Changjiang 
Published: March 2022

Abstract
Resistance to chemotherapy represents a bottleneck in clinical cancer treatment and demands urgent solutions. Dysfunction of glucose metabolism including the Warburg effect is an outstanding hallmark of aberrant cancer metabolism which remarkably fosters chemoresistance. Targeting disordered mitochondrial metabolism have paved the road for enhancing chemosensitivity. Human telomerase reverse transcriptase
(hTERT) is tightly associated with cancer initiation and progression. Notably, hTERT can unconventionally translocate from nucleus to mitochondria. However, the influence of mitochondrial hTERT on glucose metabolism and the subsequent contribution to chemoresistance remains largely elusive. Here, we uncovered that mitochondrial hTERT level is negatively with chemotherapy response in patients, and disclosed the undocumented role of mitochondrial hTERT in reprogramming cancer metabolism towards Warburg effect independent of RT activity which authentically promoted chemoresistance. Mitochondrial hTERT boosted Warburg effect and weakened oxidative phosphorylation (OXPHOS) specifically via suppressing Complex I activity.Interactome profiling by mass spectrometry revealed that mitochondrial hTERT physically interacted with Complex I core subunit MT-ND1 (ND1) through its matrix loop region to hinder Complex I functions. A novel TAT-Pep designed on the basis of their interacting region effectively abrogated the hTERT-ND1 interplay, which substantially rejuvenated Complex I activity and rewired cancer metabolism from Warburg effect towards OXPHOS thereby strengthening chemosensitivity in both
resistant cancer lines and patient-derived xenograft (PDX) tumor models. Collectively,our study reveals the interaction between MT-ND1 and hTERT as an essential mechanism that underlies the rewiring of cellular glucose metabolism and chemoresistance. Targeting mitochondrial hTERT-governed adverse metabolic
remodeling sheds light on the avenue towards better chemotherapeutics efficacy.
Effects of plasma-derived exosomes from the normal and thin camels on hepatocellular carcinoma and their differences at transcriptome and proteomics levels
Authors: Yao Hongqiang , Yu Siriguleng , Luo Yuchen , Wang Ming , Wang Xiuying , Bai Zhaoxing , Zhang Yawen , Zhang Guangjie 
Published: March 2022

Abstract
Background
Hepatocellular carcinoma (HCC) is a common malignant primary tumor. Camels have high economic and social values, but their potential medical value has not been studied. This study aimed to investigate the effects of thin and normal camel plasma-derived exosomes on HCC.
Methods
Plasma was obtained from thin and normal camels, and used to isolate exosomes by ultracentrifugation. The exosomes were then characterized by transmission electron microscopy and Nano particle tracking analyzer. In vivo imaging of nude mice and hematoxylin eosin (HE) staining of liver tissues were used to explore the effects of the exosomes on tumor growth. Finally, the differences of the two exosomes were further analyzed using small RNA sequencing and proteomics.
Results
In vivo imaging and HE staining showed that no significant differences were found in fluorescence value and liver tissue morphology between the control mice and the mice treated with the exosomes from thin camels; while the fluorescence value and the live histology changes were alleviated in the mice with the exosomes from normal camels. After sequencing and proteomic analysis, 40 DE-miRNAs (15 down-regulated and 25 up-regulated) and 172 DEPs (77 up-regulated and 95 down-regulated) were identified in the plasma-derived exosomes from normal camels. These identified DE-miRNAs and DEPs were significantly enriched in many signaling pathways.
Conclusions
Normal camel plasma-derived exosomes may inhibit the growth of HCC cells through regulating pathways of Ras, Rap1, PI3K-Akt, MAPK, AMPK, and canonical Wnt signaling pathways.
Thermal Analysis Of Graphene Based Nanofluids For Energy System And Economic Feasibility
Authors: Mohammad Reem Sabah , Aldlemy Mohammed Suleman , Falah Mayadah W. , Homod Raad Z , Yaseen Zaher Mundher 
Published: March 2022

Abstract

Thermal and economic evaluation using covalent functionalized Graphene Nanoplatelets (CF-GNPs) were performed inside a heated-pipe under turbulent conditions. The current nanofluids were produced from CF-GNPs added and distilled water in different mass percentages such as 0.025,0.05,0.075 and 0.1wt.%. The thermal system was under the condition of fully-developed turbulent flow in the range of 6,401 ≤ Re ≤ 11,907 and heated up to 11,205 W/m2. The characterization and morphological properties were done through different examinations and techniques such as zeta potential, nanoparticle sizer, Field Emission Scanning Electron Microscopy (FE-SEM), and Field Emission Transmission Electron Microscope (FE-TEM). Moreover, the economic performance was evaluated to estimate the price-operation of the current thermal system. The heat exchanger size reduced by 16.10%, 21.92%, 25.37% and 29.35% for 0.025wt.%, 0.05wt.%, 0.075wt.% and 0.1wt.%, respectively. Furthermore, the required power for base fluid was 422W then reduced to 354W, 326W, 315W and 298W for 0.025wt.%, 0.05wt.%, 0.075wt.% and 0.1wt.%, respectively.
Aqueous Preparation of Self-Supported Hydroxypropyl Methylcellulose-Polyaniline Conducting Films and the Evaluation of Their Electrical and Mechanical Properties
Authors: Maeda Vinicius Cavalheiro , Correa Cintia Marques , Hamanaka Marcos Henrique Mamoru Otsuka , Hamanaka Viviane Nogueira , Molina Celso , Camilo Fernanda F 
Published: March 2022

Abstract
The purpose of this work was to develop a simple method to produce self-supported and homogeneous films composed of hydroxypropyl methylcellulose (HPMC) and polyaniline (PANI) by the simple mixture of aqueous solutions of both polymers with subsequent drying. The addition of HPMC, a cellulose derivative with an excellent film-forming capacity, was fundamental to overcoming the poor processability of pristine PANI film, which impairs its use in many technological applications. Despite being an insulating polymer, the addition of HPMC did not significantly decrease the conductivity and all hybrid films showed conductivity in the order of 10− 2 − 10− 3 S cm− 1, which is in the range for metals or semiconductors. The typical electroactivity of PANI was also maintained with the addition of HPMC. Also, as a beneficial, adding HPMC the thermal stability and the mechanical properties of the pristine PANI were improved. Cellulose-containing conducting polymers with electroactive activity can be considered as a material of the future, with possible applications in several areas, such as smart wallpapers, e-papers, and sensors.
Design and properties of new dendritic macromolecules substituted by several triarylamines for organic photovoltaic applications based on Carbazole and Thiophene
Authors: Saidi Wafae , AGDA FATIMA , ABRAM TAYEB , Bejjit Lahcen , Bouachrine Mohammed 
Published: March 2022

Abstract
This work proposes a theoretical study of the geometric and optoelectronic properties of some dendritic macromolecules consisting mainly of thiophene and carbazole. First, calculations were performed on a basic molecule called G2TTPG2 3T using the density functional theory (DFT) and varying between 5 different quantum methods to be able to choose the most appropriate which will then be applied to the derivatives of this molecule during the rest of this work, these derivatives were obtained by grafting the following groups: CN, EDOT-S, OH, OHOH, EDOT, F. The objective of this work is to study the effect of this chemical modification, better explore the structure-property relationship and also to search for compounds with the best optoelectronic properties in order to present them for synthesis. The results obtained reveal that the substitution by some groups has reduced the energy of the gap, decreased inter-cyclic distances and increased the value of the open circuit voltage.
Small Bowel Cleanliness in Capsule Endoscopy: A Case-control Study Using Validated Artificial Intelligence Algorithm
Authors: Oh Dong Jun , Hwang Youngbae , Nam Ji Hyung , Lim Yun Jeong 
Published: March 2022

Abstract
Background: Small bowel capsule endoscopy (SBCE) may need to be performed immediately after colonoscopy without additional bowel preparation if active small bowel diseases are suspected. However, it is unclear whether the small bowel cleanliness is adequately maintained in SBCE immediately after colonoscopy. Methods: Cases of SBCE in which polyethylene glycol were used were included retrospectively. We compared the small bowel cleanliness scores using a validated artificial intelligence (AI) algorithm (cut-off score > 3.25 for adequate). Results: Among 85 enrolled cases, 50 cases (58.8%) were the study group. The mean time from last dose of purgative administration to SBCE was 6.86 ± 0.94 hours in study group and 3.00 ± 0.18 hours in control group. Seventy-five cases (88.2%) were adequate small bowel cleanliness, which was not different between the two groups. The mean small bowel cleanliness score for study group was 3.970 ± 0.603, and for control group was 3.937 ± 0.428. In the study group, better colon preparation resulted in higher the small bowel cleanliness score (p = 0.015). Conclusions: Small bowel cleanliness was also adequately maintained in SBCE immediately after colonoscopy. There was no difference between the time and volume of purgative administration and small bowel cleanliness.
Tumor-activated Neutrophils Promote Metastasis in Breast Cancer via the G-CSF-RLN2-MMP-9 Axis
Authors: Sheng Youjing , Peng Weidong , Huang Yan , Cheng Lanqing , Meng Ye , Kwantwi Louis Boafo , Yang Jiezhen , Xu Jiegou , Xiao Han , Kzhyshkowska Julia , Wu Qiang 
Published: March 2022

Abstract
Immune component of tumor microenvironment is essential for the regulation of cancer progression. Tumor mass is frequently infiltrated by neutrophils (tumor-associated neutrophils, TANs) in breast cancer (BC) patients. However, the role of TANs and the mechanisms of their action in BC are still unclear. Using quantitative IHC, ROC and Cox analysis, we demonstrate here that high density of TANs infiltrating tumor parenchyma is predictive for poor prognosis and for the decreased progression-free survival of patients with BC that underwent surgical tumor removal without previous neoadjuvant chemotherapy in all 3 cohorts (training cohort, n = 170; validation cohorts, n = 130, independent cohorts, n = 95). Conditioned medium of human breast cancer cell lines prolonged life span of healthy donor neutrophils ex vivo. Neutrophils, activated by supernatants of breast cancer lines, demonstrated the increased ability to stimulate proliferation, migration and invasive activity of breast cancer cells. Cytokines involved in this process have been identified by antibody array. Relationships between these cytokines and density of TANs were validated by ELISA and IHC in fresh breast cancer surgical samples. Identified by antibody arrays, tumor-derived G-CSF significantly prolonged the lifespan and increased the metastasis-promoting activities of neutrophils via PI3K-AKT and NFκB pathway. Simultaneously, TAN-derived RLN2 promoted the migratory abilities of MCF-7 cells via PI3K-AKT-MMP-9. Analysis of tumor tissues from 20 BC patients and RNA-seq from TCGA BC patients identified a positive correlation of density of TANs with the activation of G-CSF-RLN2-MMP-9 axis. Our data indicate that TANs in human breast cancer have detrimental activity supporting malignant cells invasion and migration.
High-normal alanine aminotransferase as a Predictor of significant liver histological injury in Chronic Hepatitis B Virus Infection with different HBV DNA levels
Authors: shan Ren , Junfeng Lu , Kefei Wang , Lina Ma , Yanhong Zheng , Sujun Zheng , Xinyue Chen 
Published: March 2022

Abstract
Objective
We aimed to assess significant liver histological injury(SLHI) of chronic hepatitis B (CHB) patients with normal ALT in order to select clinical threshold of ALT.
Methods
We retrospectively examined 347 patients who underwent liver biopsy in Beijing You an hospital, Capital Medical University from January 2018 to January 2021. Univariate analysis of the clinical parameters versus inflammation and fibrosis was carried out.
Results
Of these 347 patients met the inclusion criteria, 174(50.1%) patients had SLHI totally.The AUROC of the ALT associated with the diagnosis of SLHI was 0.572 for female and 0.6 for male with a cut-off value of 20U/L and 25U/L, respectively (p = 0.04, p = 0.013).Among HBeAg-positive patients,the proportion of SLHI in the high-normal ALT group (ALT > 20 U/L for female,>25 U/L for male) with low HBV DNA loads(< 7log10 IU/ml)was higher(66.7% vs 33.3%, p < 0.001); also the proportion in the HBeAg-negative with high-normal ALT and HBV DNA ≧ 2000 IU/ml was significantly higher(70% vs 37.7%, p < 0.001).
Conclusion
Clinical threshold of ALT to distinguish SLHI was 25U/L for male and 20U/L for female.The CHB patients with high-normal ALT and certain HBV DNA level ( ≧ 2000 IU/ml for HBeAg-negative and < 7log10 IU/ml for HBeAg-positive) were suggested to perform liver biopsy or noninvasive methods for histopathology assessment, then to be determined for antiviral therapy.
Cortical Correlates of Increased Postural Task Difficulty in Young Adults: A Combined Pupillometry and EEG Study
Authors: Kahya Melike , Liao Ke , Gustafson Kathleen , Akinwuntan Abiodun , Manor Brad , Devos Hannes 
Published: March 2022

Abstract
Objective
Pupillary response reflects mental effort (or cognitive workload) during challenging cognitive and postural control conditions. EEG is more established as a non-invasive measure to assess cortical involvement of postural control. The purpose of this study was to understand the pupillary response under increasing postural task difficulty and its relationship between EEG outcomes in young adults.
Methods
Fifteen adults [age: 25.6 ± 2.9; sex: 10 males] completed multiple trials of standing: eyes-open, eyes-open and performing a dual-task (auditory 2-back), eyes-occluded, and eyes-occluded with a dual task. Participants stood on a force plate and wore an eye tracker and 256-channel EEG cap during the conditions. The power spectrum was analyzed for theta (4–7 Hz), alpha (8–13 Hz), and beta (13–30 Hz) frequency bands in pre-defined fronto-central of electrocortical sources.
Results
Increased postural task difficulty was associated with greater responses for pupillary response (p < 0.0001), fronto-central alpha power (p = 0.001), and theta/beta power ratio (p = 0.01). Greater pupillary response correlated with lower fronto-central EEG alpha power during standing eyes occluded (r=-.58, p = .04) and dual-task eyes occluded (r=-.56, p = .04). Lastly, a greater pupillary response was associated with lower CoP displacement in the anterior-posterior direction during dual-task eyes occluded (r = − .60, p = .04)
Conclusion
Pupillary response and fronto-central EEG outcomes appear to capture the same cortical processes that are increasingly utilized during increased postural task difficulty. In addition, these results validate pupillary response against EEG during increased postural task difficulty. In the future, pupillary response may be a potential tool to understand the underlying neurophysiological mechanisms of postural control in aging and disease populations.
Screening and analyzing of Genes and Signaling Pathways Associated with Size differentiation of Adult Male Prawn Macrobrachium nipponense
Authors: Jiang Gang , Zhu Yongming , Xue Yucai , Lu Ying , Liu Zhiwei , Huang Xuxiong 
Published: March 2022

Abstract
Adult male prawn Macrobrachium nipponense exhibits heterogeneous individual growth (HIG), they could polarize into large size males and small size males which have sizes smaller than females. Therefore, obtaining the large size prawns will increase the profit of farming. To better understand this size differentiation mechanism, we performed transcriptome sequencing from the second pereiopod, hepatopancreas, and muscle of adult male prawn M. nipponense with significant differences in size (large: 1.47±0.12g; small: 0.28±0.04g). We identified 46,557 unigenes with an average length of 1312 bp. A total of 805, 473, and 1037 significantly up-regulated DEGs were identified based on LP versus SP, LM versus SM, and LH versus SH, respectively. These data indicated that hepatopancreas played stronger regulatory roles than other two tissues on size differentiation in male M. nipponense. The KEGG pathway analysis indicated that pathways PI3K-Akt pathway, cAMP pathway, Hippo pathway, and Ras signaling pathway were enriched in all three comparisons, which may be key size regulators in adult male prawn M. nipponense. Furthermore, twenty-eight DEGs involved in these pathways and their expression was verified by quantitative Real-Time PCR (qPCR). These four pathways and twenty-eight predicted potential size-related genes may be strong candidates for size differentiation in adult male M. nipponense. Finding of the study have potential applications for improving the commercial size of cultured male prawn M. nipponense.
Security-enhanced Watermark Embedding and Transmission Based on Synchronized Chaotic Semiconductor Lasers
Authors: Wang Jun , Tang Xi , Wang Fei , Lin Xiao-Dong , Zheng Qiu-Lan , Xia Guang-Qiong , Wu Zheng-Mao , Deng Tao 
Published: March 2022

Abstract
In this paper, we propose and numerically demonstrate a chaotic watermarking encryption and secure transmission scheme based on two synchronized response lasers (RLs) subject to identical injection from a driving laser (DL). In this proposed system, the chaos signals from DL can be optimized after passing through a phase modulator and a dispersion component, then the chaotic signal with large bandwidth and low TDS is obtained. Simultaneously injecting this chaotic signal into two identical RLs, high-quality chaos synchronization between two RLs can be realized. Both synchronized chaotic signals are respectively divided into two parts. One is used to generate shared keys required by the watermarking encryption, and the other is used as chaotic carrier to realize the secure message transmission. After introducing the synchronized optical chaos into the watermarking encryption algorithm, the normalized correlation of the extracted watermark without long-distance transmission is 0.9554 and can still maintain above 0.9 over 200km transmission fiber. The message transmission and watermark extraction have good robustness to the fiber parameter mismatches, and excellent performances can be maintained even within 20% mismatches range. Moreover, the security of the watermarking encryption algorithm and the message transmission can be guaranteed simultaneously.
Glucose transporter 9 regulates hepatic steatosis through modulating intrahepatic uric acid contents in mice
Authors: Zeng Hang , Tang Chenxi , Lin Bingru , Yu Chaohui 
Published: March 2022

Abstract
Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide. The important role of urid acid (UA) in NAFLD has been widely investigated. Our previous studies unveiled the elevation of serum UA levels independently predicts an increased risk of incident NAFLD. However, the role of intrahepatic UA in NALFD has not been investigated yet. Glucose transporter 9 (GLUT9) is a key transporter that mediates the uptake of UA in hepatocytes. Methods: In this study, we first explored the clinical association between GLUT9 polymorphism and NAFLD. Blood samples of 247 male Chinese (127 were NAFLD patients) were collected and tested for the blood UA levels and genotype of the single nucleotide polymorphism (SNP) of GLUT9 (rs1014290). Next, Glut9 hepatic-specific knockout mice (Glut9Hep-ko) were generated to investigate the role of hepatic GLUT9 in NAFLD in male mice. Results: We found that the GA/AA genotypes (rs1014290) were associated with elevated serum UA levels in NAFLD patients. Meanwhile, we found that Glut9Hep-ko mice displayed lower intrahepatic UA levels, down-regulated lipogenesis genes expressions, and attenuated NAFLD symptoms after 12 weeks of high fat diet feeding, compared with Glut9Fl/Fl littermates. However, Glut9Hep-ko mice and wild type littermates showed no significant difference on hepatic fatty acid oxidation or inflammation. Conclusions: our results suggested that GLUT9 polymorphism was significantly associated with NAFLD, and hepatic-specific knockout of Glut9 significantly decreased intrahepatic contents and ameliorated diet-induced NAFLD in mice.
Spin Coated Ag NPs SERS substrate: Role of electromagnetic and chemical enhancement in trace detection of methylene blue and congo red
Authors: Shaikh Imran , Haque Mohammed Abdul , Pathan Habib , Sartale Shrikrishna Dattatraya 
Published: March 2022

Abstract
This work demonstrates a simple low cost and reproducible spin coating method followed by thermal reduction for the uniform growth of silver nanoparticles (AgNPs) as surface enhanced Raman scattering (SERS) substrate. In this work, cationic dye MB and anionic dye CR are selected as two different kinds of SERS probe molecules to investigate the differences between EME and CE mechanism. The results indicate that SERS spectra of MB molecule on AgNPs SERS substrate exhibit the shift in the Raman peak positions and high sensitivity (LOD ~ 10− 10 M) with large GSERS (~ 109) value, which can be attributed to the contribution from both EME and CE mechanism. It is found that Cl− halide ions present in the MB molecule are forming surface complex (AgNPs@Cl−) on the surface of AgNPs SERS substrate which is confirmed by the XPS investigation, which might be responsible for the CE enhancement in MB molecule. Whereas Congo red being anionic dye showed no shift in Raman peak positions and less sensitivity (LOD ~ 10− 8) with GSERS (~ 107) value on AgNPs SERS substrate, which can be attributed to EME mechanism only. The obtained results suggest that Cl− halide ions present in the MB molecule plays important role by giving large SERS enhancement for MB molecule on AgNPs SERS substrate. It is expected that this work will provide insight into role of EME and CE mechanisms for cationic dye MB and anionic dye CR.