bluuebunny/crossref_metadata_2025_split huggingface dataset:
GEQO: The Hub of Organometallic Chemistry in the Spanish Royal Society of Chemistry
Authors: Albéniz Ana C. 
Published: March 2022

AbstractThe Specialized Group of Organometallic Chemistry (Grupo Especializado de Química Organometálica, GEQO) was created within the Spanish Royal Society of Chemistry (RSEQ) in 1981, and organometallic chemistry research has grown immensely in Spain since then. Guest Editor Ana Albéniz, EurJIC’s Editorial Board Chair and president of the GEQO, briefly introduces the Specialized Group of Organometallic Chemistry (Grupo Especializado de Química Organometálica, GEQO) of the Spanish Royal Society of Chemistry (RSEQ) and presents EurJIC’s RSEQ‐GEQO Prizewinners Special Collection.
SnCl2‐Catalyzed Acetalation/Selective Benzoylation Sequence for the Synthesis of Orthogonally Protected Glycosyl Acceptors
Authors: Lv Jian , Liu Chun‐Yang , Guo Yang‐Fan , Feng Guang‐Jing , Dong Hai 
Published: March 2022

AbstractBased on SnCl2‐catalyzed acetalation and selective benzoylation, a one‐pot strategy to efficiently synthesize orthogonally protected glycosyl acceptors with 2‐OH/3‐OH was developed. Consequently, 2‐OBz or 3‐OBz 4,6‐O‐benzylidene galactosides and glucosides were efficiently prepared in moderate to high yields starting from free galactosides and glucosides, and were used as valuable glycosyl acceptors for the synthesis of blood group antigens O and B analogues in this study.
Global intercompany assessment of ICIEF platform comparability for the characterization of therapeutic proteins
Authors: Madren Seth , McElroy Will , Schultz‐Kuszak Kristin , Boumajny Boris , Shu Yao , Sautter Sabine , Zhao Helen C. , Schadock‐Hewitt Abby , Chumsae Chris , Ball Nancy , Zhang Xiaoying , Rish Kimberly , Zhang Shukui , Wurm Christine , Cai Sumin , Bauer Scott P. , Stella Cinzia , Zheng Laura , Roper Brian , Michels David A. , Wu Gang , Kocjan Bostjan , Birk Matej , Erdmann Simon Erik , He Xiaoping , Whittaker Brad , Song Yvonne , Barrett Hannah , Strozyk Kevin , Jing Ye , Huang Long , Mhatre Vishal , McLean Paul , Yu Tiantian , Yang Huijuan , Mattila Minna 
Published: March 2022

AbstractAn international team spanning 19 sites across 18 biopharmaceutical and in vitro diagnostics companies in the United States, Europe, and China, along with one regulatory agency, was formed to compare the precision and robustness of imaged CIEF (ICIEF) for the charge heterogeneity analysis of the National Institute of Standards and Technology (NIST) mAb and a rhPD‐L1‐Fc fusion protein on the iCE3 and the Maurice instruments. This information has been requested to help companies better understand how these instruments compare and how to transition ICIEF methods from iCE3 to the Maurice instrument. The different laboratories performed ICIEF on the NIST mAb and rhPD‐L1‐Fc with both the iCE3 and Maurice using analytical methods specifically developed for each of the molecules. After processing the electropherograms, statistical evaluation of the data was performed to determine consistencies within and between laboratory and outlying information. The apparent isoelectric point (pI) data generated, based on two‐point calibration, for the main isoform of the NIST mAb showed high precision between laboratories, with RSD values of less than 0.3% on both instruments. The SDs for the NIST mAb and the rhPD‐L1‐Fc charged variants percent peak area values for both instruments are less than 1.02% across different laboratories. These results validate the appropriate use of both the iCE3 and Maurice for ICIEF in the biopharmaceutical industry in support of process development and regulatory submissions of biotherapeutic molecules. Further, the data comparability between the iCE3 and Maurice illustrates that the Maurice platform is a next‐generation replacement for the iCE3 that provides comparable data.
Impact of soil‐structure interaction on the effectiveness of Tuned Mass Dampers
Authors: Gorini Davide Noè , Chisari Corrado 
Published: March 2022

AbstractTuned Mass Dampers (TMDs) can represent an attracting solution to mitigate vibrations of a structure under seismic excitation, but their effectiveness can be considerably altered by the dynamic interaction with the foundation soil. The available design criteria for TMDs do not account for these effects and can therefore lead to a non‐optimised structural performance. In this paper an investigation on the dynamic interaction of the TMD with the whole soil‐structure system is presented, with the objective of highlighting the system parameters governing the response and the effectiveness of the device as seismic protection. An interpretative model of the soil‐structure‐TMD system expressed in a rigorous non‐dimensional form is proposed, and an extensive global sensitivity analysis on its performance under harmonic loading is carried out. The identification of the typical performance regions shows that the seismic effectiveness of a TMD is mainly controlled by a limited number of parameters describing the structural behaviour and the soil‐structure interaction, such as the structure‐to‐soil relative stiffness and those governing foundation rocking. The non‐dimensional system parameters leading to either a favourable or detrimental effect on the TMD performance due to soil‐structure interaction are also identified, and two design methodologies proposed in the literature are critically assessed in light of the framework proposed.
Investigation of sintering properties and ceramifying process of amorphous silica/mica‐based ceramifiable polyethylene composites
Authors: Shen Jiapei , Sun Qing , Li Lang , Zhang Jian , Sheng Jiawei 
Published: March 2022

SummaryCeramifiable polyolefin‐based composites are promising materials for fire‐resistant cables. Many studies have been focused on improving the ceramifiable properties by developing various composite matrixes with optimum inorganic fillers. Few studies have been carried out to compare the sintering properties of ceramifying powder (CP) and ceramifiable polymer‐based composites directly. In this work, a low‐temperature sintering CP, formulated with mica, amorphous silica and glass frits, and filled into polyethylene (PE) matrix to prepare a ceramifiable PE composites (CPE). The sintering properties, including the flexural strength, apparent porosity and volume variation, were tested at calcination temperatures from 600 to 900°C. Results showed that the CP had better sintering properties than the CPE at the same calcined temperatures, but the gap between the two continued to decrease as the temperature increased. The negative effect of PE matrix decomposition on the ceramifying process was more pronounced in the low‐temperature ranges (600 and 700°C). As the temperature reached above 700°C, the sintering of the CP was accelerated, and the sintering properties of composite residue increased significantly. The ceramifying process of the CPE was investigated by thermalgravimetric analysis, X‐ray diffraction and scanning electron microscopy. The analyses demonstrated that the induction of the CP improved the thermal stability of the CPE, and played the main role in the structure evolution of the composite residue. During the ceramifying process, the glass frits melted at low temperatures, bridging mica and amorphous silica. As the temperature increased to 800 and 900°C, mica had a eutectic reaction with the glass liquid phase, facilitating the change of a bridging structure to the continuous island structure responsible for the superior mechanical and fire‐resistant properties of the CPE.
Single‐Cell Transcriptome Identifies Drug‐Resistance Signature and Immunosuppressive Microenvironment in Metastatic Small Cell Lung Cancer
Authors: Zhang Jing , Zhang Haiping , Zhang Lele , Li Dianke , Qi Mengfan , Zhang Liping , Yu Huansha , Wang Di , Jiang Gening , Wang Xujun , Zhu Xianmin , Zhang Peng 
Published: March 2022

AbstractSmall cell lung cancer (SCLC) is a deadly neuroendocrine malignancy with high metastasis. However, the heterogeneity of metastatic SCLC at the single‐cell level remains elusive. The single‐cell transcriptome of a total of 24 081 cells in metastatic lymph node samples from seven SCLC patients via endobronchial ultrasound‐guided transbronchial needle aspiration (EBUS‐TBNA) is examined. Genomic alterations are also examined by whole exome sequencing (WES) and the immune infiltration between SCLC and non‐SCLC (NSCLC) is compared using public single‐cell RNA sequencing (scRNA‐seq) data. It is identified that malignant cells in lymph‐node metastatic SCLC have inter‐patient and intra‐tumor heterogeneity characterized by distinct ASCL1 and NEUROD1 expression patterns. High expression of genes such as FZD8 in WNT pathway is associated with drug resistance in malignant cells. Compared to NSCLC, SCLC harbors a unique immunosuppressive tumor microenvironment. Malignant cells exhibit a pattern of attenuated MHC‐I antigen presentation‐related gene expression, which is associated with relatively low proportion of exhausted T cells. Natural killer (NK) cells display impaired antitumor function with high expression of TGFBR2. This work characterizes the inter‐patient and intra‐tumor heterogeneity of metastatic SCLC and uncovers the exhaustion signatures in NK cells, which may pave the way for novel treatments for SCLC including immune checkpoint blockade‐based immunotherapy.
Privacy‐preserving quality control of neuroimaging datasets in federated environments
Authors: Saha Debbrata K. , Calhoun Vince D. , Du Yuhui , Fu Zening , Kwon Soo Min , Sarwate Anand D. , Panta Sandeep R. , Plis Sergey M. 
Published: March 2022

AbstractPrivacy concerns for rare disease data, institutional or IRB policies, access to local computational or storage resources or download capabilities are among the reasons that may preclude analyses that pool data to a single site. A growing number of multisite projects and consortia were formed to function in the federated environment to conduct productive research under constraints of this kind. In this scenario, a quality control tool that visualizes decentralized data in its entirety via global aggregation of local computations is especially important, as it would allow the screening of samples that cannot be jointly evaluated otherwise. To solve this issue, we present two algorithms: decentralized data stochastic neighbor embedding, dSNE, and its differentially private counterpart, DP‐dSNE. We leverage publicly available datasets to simultaneously map data samples located at different sites according to their similarities. Even though the data never leaves the individual sites, dSNE does not provide any formal privacy guarantees. To overcome that, we rely on differential privacy: a formal mathematical guarantee that protects individuals from being identified as contributors to a dataset. We implement DP‐dSNE with AdaCliP, a method recently proposed to add less noise to the gradients per iteration. We introduce metrics for measuring the embedding quality and validate our algorithms on these metrics against their centralized counterpart on two toy datasets. Our validation on six multisite neuroimaging datasets shows promising results for the quality control tasks of visualization and outlier detection, highlighting the potential of our private, decentralized visualization approach.
mRNA and gene editing: Late breaking therapies in liver diseases
Authors: Zabaleta Nerea , Torella Laura , Weber Nicholas D. , Gonzalez‐Aseguinolaza Gloria 
Published: March 2022

Abstract
The efficient delivery of RNA molecules to restore the expression of a missing or inadequately functioning protein in a target cell and the intentional specific modification of the host genome using engineered nucleases represent therapeutic concepts that are revolutionizing modern medicine. The initiation of several clinical trials using these approaches to treat metabolic liver disorders as well as the recently reported remarkable results obtained by patients with transthyretin amyloidosis highlight the advances in this field and show the potential of these therapies to treat these diseases safely and efficaciously. These advances have been possible due, firstly, to significant improvements made in RNA chemistry that increase its stability and prevent activation of the innate immune response and, secondly, to the development of very efficient liver‐targeted RNA delivery systems. In parallel, the breakout of CRISPR/CRISPR‐associated 9–based technology in the gene editing field has marked a turning point in in vivo modification of the cellular genome with therapeutic purposes, which can be based on gene supplementation, correction, or silencing. In the coming years we are likely to witness the therapeutic potential of these two strategies both separately and in combination. In this review we summarize the preclinical data obtained in animal models treated with mRNA as a therapeutic agent and discuss the different gene editing strategies applied to the treatment of liver diseases, highlighting both their therapeutic efficacy as well as safety concerns.
Ten‐eleven translocation‐2 inactivation restrains IL‐10‐producing regulatory B cells to enable antitumor immunity in hepatocellular carcinoma
Authors: Lu Zhou , Liu Ronghua , Wang Yining , Jiao Mengxia , Li Zhongchen , Wang Zhiqiang , Huang Cheng , Shi Guoming , Ke Aiwu , Wang Luman , Fu Ying , Xia Jie , Wen Haoyu , Zhou Jian , Wang Xiaoying , Ye Dan , Fan Jia , Chu Yiwei , Cai Jiabin 
Published: March 2022

Background and Aims:
IL‐10‐producing regulatory B cells (IL‐10+B cells), a dominant regulatory B cell (Breg) subset, foster tumor progression. However, the mechanisms underlying their generation in HCC are poorly understood. Ten‐eleven translocation‐2 (TET2), a predominant epigenetic regulatory enzyme in B cells, regulates gene expression by catalyzing demethylation of 5‐methylcytosine into 5‐hydroxymethyl cytosine (5hmC). In this study, we investigated the role of TET2 in IL‐10+B cell generation in HCC and its prospects for clinical application.

Approach and Results:
TET2 activation in B cells triggered by oxidative stress from the HCC microenvironment promoted IL‐10 expression, whereas adoptive transfer of Tet2‐deficient B cells suppressed HCC progression. The aryl hydrocarbon receptor is required for TET2 to hydroxylate Il10. In addition, high levels of IL‐10, TET2, and 5hmc in B cells indicate poor prognosis in patients with HCC. Moreover, we determined TET2 activity using 5hmc in B cells to evaluate the efficacy of anti‐programmed death 1 (anti‐PD‐1) therapy. Notably, TET2 inhibition in B cells facilitates antitumor immunity to improve anti‐PD‐1 therapy for HCC.

Conclusions:
Our findings propose a TET2‐dependent epigenetic intervention targeting IL‐10+B cell generation during HCC progression and identify the inhibition of TET2 activity as a promising combination therapy with immune checkpoint inhibitors for HCC.
The effect of probiotics on gestational diabetes and its complications in pregnant mother and newborn: A systematic review and meta‐analysis during 2010–2020
Authors: Mahdizade Ari Marzie , Teymouri Samane , Fazlalian Tayebeh , Asadollahi Parisa , Afifirad Roghayeh , Sabaghan Mohammad , Valizadeh Fateme , Ghanavati Roya , Darbandi Atieh 
Published: March 2022

AbstractThis study was aimed to evaluate the effect of probiotics consumption on gestational diabetes (GD) and its complications in pregnant mother and newborn. The study was registered on PROSPERO (CRD42021243409) and all the enrolled articles were collected from four databases (Medline, Scopus, Embase, and Google Scholar) as randomized controlled trials (RCTs) from 2010 to 2020. A total of 4865 study participants from 28 selected studies were included in this review. The present meta‐analysis showed that the consumption of probiotics supplementation has the potential to decrease GD‐predisposing metabolic parameters such as blood glucose level, lipid profile, inflammation, and oxidative markers which may reduce GD occurrence among pregnant women.
Blood long non‐coding RNA intersectin 1–2 is highly expressed and links with increased Th17 cells, inflammation, multiple organ dysfunction, and mortality risk in sepsis patients
Authors: Huang Qinghe , Wang Yibin , He Fuyun 
Published: March 2022

AbstractBackgroundLong non‐coding RNA intersectin 1–2 (lnc‐ITSN1‐2) exacerbates inflammation and promotes T‐helper (Th) cell differentiation, also serves as a biomarker in critical illness diseases. However, its clinical role in sepsis remains obscure. Hence, the study aimed to explore the relationship of lnc‐ITSN1‐2 with Th cells, inflammation, disease severity, multiple organ dysfunction, and mortality risk in sepsis.MethodsPeripheral blood mononuclear cells (PBMC) were isolated from 95 sepsis patients and 50 health controls, followed by lnc‐ITSN1‐2 evaluation using RT‐qPCR. PBMC Th1, Th17 cells and their secreted cytokines in serum were detected by flow cytometry and ELISA, respectively.ResultsLnc‐ITSN1‐2 in sepsis patients was higher than it in health controls (Z = −7.328, p < 0.001). Lnc‐ITSN1‐2 correlated with increased interferon‐gamma (p = 0.009), Th17 cells (p = 0.022), and interleukin‐17A (p = 0.006), but not Th1 cells (p = 0.169) in sepsis patients. Moreover, lnc‐ITSN1‐2 had a positive connection with C‐reactive protein (p = 0.001), acute pathologic and chronic health evaluation (APACHE) II (p = 0.024), and sequential organ failure assessment (SOFA) scores (p = 0.022). Regarding SOFA subscales, lnc‐ITSN1‐2 linked with elevated respiratory system score (p = 0.005), cardiovascular system score (p = 0.007), and renal system score (p = 0.004) but no other subscales. Besides, lnc‐ITSN1‐2 had an increasing trend, but no statistical difference, in septic deaths compared to survivors (Z = −1.852, p = 0.064).ConclusionLnc‐ITSN1‐2 reflects sepsis progression and unfavorable prognosis to some extent, which may serve as a potential biomarker to improve the management of sepsis patients.
ACSL4 contributes to ferroptosis‐mediated rhabdomyolysis in exertional heat stroke
Authors: He Sixiao , Li Ru , Peng Yanmei , Wang Ziqing , Huang Junhao , Meng Hongen , Min Junxia , Wang Fudi , Ma Qiang 
Published: March 2022

AbstractBackgroundRhabdomyolysis (RM) is a common complication of exertional heat stroke (EHS) and constitutes a direct cause of death. However, the mechanism underlying RM following EHS remains unclear.MethodsThe murine EHS model was prepared by our previous protocol. RNA sequencing is applied to identify the pathological pathways that contribute to RM following EHS. Inhibition of the acyl‐CoA synthetase long‐chain family member 4 (ACSL4) was achieved by RNA silencing in vitro prior to ionomycin plus heat stress exposure or pharmacological inhibitors in vivo prior to heat and exertion exposure. The histological changes, the iron accumulation, oxidized phosphatidylethanolamines species, as well as histological evaluation and levels of lipid metabolites in skeletal muscle tissues were measured.ResultsWe demonstrated that ferroptosis contributes to RM development following EHS. Ferroptosis inhibitor ferrostatin‐1 administration once EHS onset significantly ameliorated the survival rate of EHS mice from 35.357% to 52.288% within 24 h after EHS (P = 0.0028 compared with control) and markedly inhibited RM development induced by EHS. By comparing gene expression of between sham heat rest (SHR) (n = 3) and EHS (n = 3) mice in the gastrocnemius (Gas) muscle tissue, we identified that Acsl4 mRNA expression is elevated in Gas muscle tissue of EHS mice (P = 0.0038 compared with SHR), so as to its protein levels (P = 0.0001 compared with SHR). Followed by increase in creatine kinase (CK) and myoglobin (MB) levels, the labile iron accumulation, decrease in glutathione peroxidase 4 (GPX4) expression, and elevation of lipid peroxidation products. From in vivo and in vitro experiments, inhibition of Acsl4 significantly improves muscle cell death caused by EHS, thereby ameliorating RM development, followed by reduction in CK and MB levels by 30–40% (P < 0.0001; n = 8–10) and 40% (P < 0.0001; n = 8–10), restoration of GPX4 expression, and decrease in lipid peroxidation products. Mechanistically, ACSL4‐mediated RM seems to be Yes‐associated protein (YAP) dependent via TEA domain transcription factor1/TEA domain transcription factor4.ConclusionsThese findings demonstrate an important role of ACSL4 in mediating ferroptosis activation in the development of RM following EHS and suggest that targeting ACSL4 may represent a novel therapeutic strategy to limit the skeletal muscle cell death and prevent RM after EHS.
Highly exo selective, photochemically promoted cyclization of iodoallene derivatives
Authors: Jovanovic Milos , Simic Milena , Petkovic Milos , Tasic Gordana , Maslak Veselin , Jovanovic Predrag , Savic Vladimir 
Published: March 2022

AbstractA photochemically promoted intramolecular cyclization of aryl‐, vinyl‐, and alkyliodo allenes has been developed. The optimal conditions employed [Ir(ppy)2(dtbbpy)]PF6 (1 mol%) as catalyst affording products with high exo selectivity in moderate to good yields. Chiral substrates showed diastereoselectivity of up to 95/5 favoring trans product.
Delineating the epilepsy phenotype of NGLY1 deficiency
Authors: Levy Rebecca J. , Frater Christina H. , Gallentine William B. , Phillips Jennifer M. , Ruzhnikov Maura R. 
Published: March 2022

AbstractWe delineated the phenotypic spectrum of epilepsy in individuals with NGLY1 deficiency from an international cohort. We collected detailed clinical and electroencephalographic data from 29 individuals with bi‐allelic (likely) pathogenic variants in NGLY1 as part of an ongoing prospective natural history study. Participants were evaluated in‐person at a single center and/or remotely. Historical medical records were reviewed. Published cases were included for comprehensive phenotyping. Of 29 individuals (mean 11.4 years, range 3–27 years), 17 (58.6%) participants had a history of epilepsy. Seizure onset was in early childhood (mean 43 months, range 2 months to 19 years). The most common seizure types were myoclonic and atonic. Epilepsy course was variable, but 35.2% (6/17) of participants with epilepsy achieved seizure freedom. The most common medications included levetiracetam, valproate, lamotrigine, and clobazam. Electroencephalogram (EEGs) were abnormal in 80% (12/15) of participants with or without epilepsy, although encephalopathy was uncommon. There was a trend in neurodevelopmental outcomes that participants with epilepsy had more developmental delays. In summary, epilepsy is common in NGLY1 deficiency. Over half of the participants had a history of epilepsy and nearly all had EEG abnormalities indicating an increased risk of epilepsy. This work expands the electroclinical phenotype of NGLY1 deficiency and supports a high clinical suspicion for seizures. Some of the more common seizure types (epileptic spasms, myoclonic, and atonic seizures) can be subtle and require counseling to ensure early recognition and treatment to ensure the best possible outcomes. Despite transient liver enzyme abnormalities in this disorder, hepatically metabolized medications were well tolerated.