bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tunable Synthesis of 1,2,3‐Triazoles and Enamines through Deacylative Azide‐Alkene Cycloaddition
Authors: Rengasamy R. , Raj J. Paul , Vijayalakshmi K. , Punitha N. , Kesavan M. , Vajjiravel M. , Elangovan Jebamalai 
Published: March 2022

AbstractA tunable synthesis of 1,4,5‐trisubstituted 1,2,3‐triazoles and enamines through [3+2] cycloaddition of activated olefins and organic azides is reported. Triazoles were achieved from benzylidene diketones and organic azides by copper oxide nanoparticle catalysis while enamines were synthesized from benzylidene malonates and organic azides under solvent‐free and catalyst‐free condition.
Diversity‐Oriented Synthesis of 2‐Iminothiazolidines: Pushing the Boundaries of the Domino Nucleophilic Displacement/Intramolecular anti‐Michael Addition Process
Authors: Ferreira Misael , Munaretto Laiéli S. , Gouvêa Daniela P. , Bortoluzzi Adailton J. , Sá Marcus M. 
Published: March 2022

AbstractMethods for the synthesis of a variety of functionalized 2‐iminothiazolidines and related heterocycles through a base‐mediated domino nucleophilic displacement/intramolecular anti‐Michael addition process involving electron‐deficient allylic bromides and 1,3‐ambident nucleophiles derived from thiourea were developed. These transformations proceed under mild and simple conditions and different functional groups are well tolerated. The scope and limitations of this protocol are dependent on the structure of the allylic bromide and 1,3‐ambident nucleophile involved. The synthetic versatility of 2‐iminothiazolidines was further demonstrated through a series of chemoselective modifications, giving rise to a wide range of thiazolidine frameworks of structural complexity. In particular, the reductive cyclization of 2‐nitrobenzyl‐substituted 2‐iminothiazolidines furnished novel spiroquinolones in good to fair yields. The structural assignment of key products was unequivocally achieved by X‐ray diffraction analysis and two‐dimensional NMR techniques.
Artificial intelligence to evaluate postoperative pain based on facial expression recognition
Authors: Fontaine Denys , Vielzeuf Valentin , Genestier Philippe , Limeux Pascal , Santucci‐Sivilotto Serena , Mory Emmanuel , Darmon Nelly , Lanteri‐Minet Michel , Mokhtar May , Laine Melanie , Vistoli Damien ,   for the DEFI study group
Published: March 2022

AbstractBackgroundPain intensity evaluation by self‐report is difficult and biased in non‐communicating people, which may contribute to inappropriate pain management. The use of artificial intelligence (AI) to evaluate pain intensity based on automated facial expression analysis has not been evaluated in clinical conditions.MethodsWe trained and externally validated a deep‐learning system (ResNet‐18 convolutional neural network) to identify and classify 2810 facial expressions of 1189 patients, captured before and after surgery, according to their self‐reported pain intensity using numeric rating scale (NRS, 0–10). AI performances were evaluated by accuracy (concordance between AI prediction and patient‐reported pain intensity), sensitivity and specificity to diagnose pain ≥4/10 and ≥7/10. We then confronted AI performances with those of 33 nurses to evaluate pain intensity from facial expression in the same situation.ResultsIn the external testing set (120 face images), the deep learning system was able to predict exactly the pain intensity among the 11 possible scores (0–10) in 53% of the cases with a mean error of 2.4 points. Its sensitivities to detect pain ≥4/10 and ≥7/10 were 89.7% and 77.5%, respectively. Nurses estimated the right NRS pain intensity with a mean accuracy of 14.9% and identified pain ≥4/10 and ≥7/10 with sensitivities of 44.9% and 17.0%.ConclusionsSubject to further improvement of AI performances through further training, these results suggest that AI using facial expression analysis could be used to assist physicians to evaluate pain and detect severe pain, especially in people not able to report appropriately their pain by themselves.SignificanceThese original findings represent a major step in the development of a fully automated, rapid, standardized and objective method based on facial expression analysis to measure pain and detect severe pain.
Construction of Cu2O@Cu1.75S Core‐shell Octahedrons for Enhanced NO2Gas Sensing at Low Temperature
Authors: Zhang Xiaohui , Li Ping , Zhang Ziwei , Guo Jinghan , Fang Zhongying , Ligani Fereja Shemsu , Liu Kaifan , Tong Xinjie , Zheng Yue , Li Zongjun , Chen Wei 
Published: March 2022

AbstractIn this work, fabrication of Cu2O@Cu1.75S core‐shell octahedrons was achieved through a facile water bath stirring process. Compared with pure Cu2O, the composite showed enhanced sensing performance for NO2. The response value of Cu2O@Cu1.75S to 5 ppm NO2at 40 °C is seven times that of Cu2O to 5 ppm NO2at 75 °C. Moreover, Cu2O@Cu1.75S has the ability to detect 100 ppb NO2and has good NO2sensing selectivity and long‐term stability. This study shows that Cu2O@Cu1.75S hybrid is a type of outstanding NO2gas sensing material on account of its low working temperature and high sensing sensitivity.
Monitoring of renewable energy systems by IoT‐aided SCADA system
Authors: Qays Md Ohirul , Ahmed Musse M. , Parvez Mahmud M. A. , Abu‐Siada Ahmed , Muyeen S. M. , Hossain Md Liton , Yasmin Farhana , Rahman Md Momtazur 
Published: March 2022

AbstractWith the rapid increase of renewable energy generation worldwide, real‐time information has become essential to manage such assets, especially for systems installed offshore and in remote areas. To date, there is no cost‐effective condition monitoring technique that can assess the state of renewable energy sources in real‐time and provide suitable asset management decisions to optimize the utilization of such valuable assets and avoid any full or partial blackout due to unexpected faults. Based on the Internet of Things scheme, this paper represents a new application for the Supervisory Control and Data Acquisition (SCADA) system to monitor a hybrid system comprising photovoltaic, wind, and battery energy storage systems. Electrical parameters such as voltage, current, and power are monitored in real‐time via the ThingSpeak website. Network operators can control components of the hybrid power system remotely by the proposed SCADA system. The SCADA system is interfaced with the Matlab/Simulink software tool through KEPServerEX client. For cost‐effective design, low‐cost electronic components and Arduino Integrated Development Environment ATMega2560 remote terminal unit are employed to develop a hardware prototype for experimental analysis. Simulation and experimental results attest to the feasibility of the proposed system. Compared with other existing techniques, the developed system features advantages in terms of reliability and cost‐effectiveness.
Identifying FecB genotypes in the muscle from sheep breeds indigenous to Xilingol, and establishment of a TaqMan real‐time PCR technique to distinguish FecB alleles
Authors: Guo Liang , Li Chun‐Dong , Liu Guo‐Qiang , Luo Jian‐Xing , Xu Wei‐Liang , Guo Yuan‐Sheng 
Published: March 2022

AbstractThe muscle from Xilingol indigenous sheep breeds are famous in China, and the FecB genotype in this population remains uncharacterized. In this study, SNPs in the FecB locus were investigated by pyrosequencing, and an optimized PCR‐RFLP technique was generated to identify SNPs. In addition, an efficient technique for high‐throughput identification of SNPs in FecB was optimized using TaqMan real‐time PCR and breed‐conservative primers and SNP‐specific probes. By genotyping the FecB locus in the muscle of Xilingol indigenous sheep breeds using a novel TaqMan real‐time PCR assay, our study has generated the groundwork for the authentication of Xilingol mutton based on the specific gene and the prolificacy‐oriented breeding of Xilingol sheep using marker‐assisted selection strategies in the future.
The implicated clinical factors for outcomes in 304 patients with salivary duct carcinoma: Multi‐institutional retrospective analysis in Japan
Authors: Kusafuka Kimihide , Sato Yoko , Nakatani Eiji , Baba Satoshi , Maeda Matsuyoshi , Yamanegi Koji , Ueda Kaori , Inagaki Hiroshi , Otsuki Yoshiro , Kuroda Naoto , Suzuki Kensuke , Iwai Hiroshi , Imamura Yoshiaki , Itakura Junya , Yamanaka Shoji , Takahashi Hideaki , Ito Ichiro , Akashi Takumi , Daa Tsutomu , Hamada Mei , Yasuda Masanori , Kawata Ryo , Yamamoto Hidetaka , Tachibana Yuri , Fukuoka Junya , Muramatsu Aya , Arai Kazumori , Suzuki Makoto 
Published: March 2022

AbstractBackgroundSalivary duct carcinoma (SDC) is a high‐grade salivary malignancy that frequently occurs as the carcinomatous component of carcinoma ex pleomorphic adenoma. We herein examined the clinical factors affecting outcomes in a large cohort of SDC.MethodsWe selected 304 SDC cases and investigated clinical characteristics and the factors affecting outcomes.ResultsThe median age of the cases examined was 68 years, the most common primary site was the parotid gland (238 cases), and there was a male predominance (M/F = 5:1). Outcomes were significantly worse when the primary tumor site was the minor salivary glands (SG) than when it was the major SG. Outcomes were also significantly worse in pN(+) cases (161 cases) than in pN0 cases, particularly those with a metastatic lymph node number ≥11. The cumulative incidence of relapse and distant metastases was significantly higher in stage IV cases than in stage 0–III cases.ConclusionsThe absolute number of lymph node metastases, higher stages, and the minor SG as the primary tumor site were identified as factors affecting the outcome of SDC.
Outcome of extracorporeal photopheresis in mycosis fungoides patients is not predicted by quotients of systemic immune‐inflammatory biomarkers
Authors: Gambichler Thilo , Späth Jan , Said Shayda , Scheel Christina H. , Susok Laura , Stranzenbach Rene 
Published: March 2022

AbstractBackgroundSystemic immune‐inflammatory biomarkers (SIIBs) have not been studied in mycosis fungoides (MF) patients undergoing extracorporeal photopheresis (ECP).ObjectiveThe objective was to determine whether recently proposed SIIBs are suitable to predict ECP treatment outcome and overall prognosis of patients with MF.MethodsTwenty‐nine MF patients were retrospectively evaluated who had undergone ECP. SIIBs included neutrophil‐to‐lymphocyte ratio, platelet‐to‐lymphocyte ratio, lymphocyte‐to‐monocyte ratio, and pan‐immune‐inflammation value.ResultsLymphocyte count (P = .021), CD4+/CD8+ cells (P = .00006), CD4+/CD56+ NK cells (P = .00008), and LDH levels (P = .0041) significantly declined after 6‐month ECP. We could not detect significant cutoff values for baseline SIIBs capable of predicting advanced disease, overall response to 6‐month ECP, or 5‐year lymphoma‐specific (LS) survival (P > .05). Circulating baseline counts of CD4+/CD7‐ cells (cutoff: ≤ 12.2; P = .010) and CD4+/CD26‐ cells (cutoff: ≤ 19.5; P < .0001) significantly predicted ECP treatment response after 6 months. Moreover, CD4+/CD8+ ratio (cutoff: > 1.34; P = .045) and increased thrombocyte counts (cutoff: >259 000; P = .010) were baseline predictors for 5‐year LS death.ConclusionECP appears to be beneficial in early‐stage CTCL as well. Lower percentages of circulating CD4+/CD7‐ and CD4+/CD26‐ lymphocytes at baseline correlate with response to ECP. In this study, however, baseline SIIBs did not appear to serve as suitable biomarkers for the prediction of treatment outcome and LS survival.
MALT1 in asthma children: A potential biomarker for monitoring exacerbation risk and Th1/Th2 imbalance‐mediated inflammation
Authors: Liu Liying , Gao Yang , Si Yonghua , Liu Baohuan , Liu Xingyue , Li Guanhui , Wang Rong 
Published: March 2022

AbstractBackgroundMucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1) participates in the immune‐related allergic response and inflammation flare, while its clinical role in asthma children is still unknown. Herein, this study aimed to investigate MALT1 expression, and its correlation with exacerbation risk, T helper (Th)1, Th2 cells (and their secreted cytokines), as well as inflammatory cytokines in asthma children.MethodsSixty children with asthma exacerbation and 60 children with remission asthma were enrolled in this study; then their blood MALT1, Th1, Th2 cells, tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), interferon‐gamma (IFN‐γ), and interleukin‐4 (IL‐4) were detected. Besides, blood MALT1 in another 20 health controls was also determined.ResultsMucosa‐associated lymphoid tissue lymphoma translocation protein 1 was highest in children with asthma exacerbation, followed by children with remission asthma, and lowest in health controls (p < 0.001). MALT1 could distinguish children with asthma exacerbation from children with remission asthma (area under the curve (AUC): 0.757, 95% CI: 0.670–0.843). In children with asthma exacerbation, MALT1 was negatively linked with IFN‐γ (p = 0.002) and Th1 cells (p = 0.050), but positively related to Th2 cells (p = 0.027) and exhibited a positive correlation trend (without statistical significance) with IL‐4 (p = 0.066); meanwhile, MALT1 was positively correlated with exacerbation severity (p = 0.010) and TNF‐α (p = 0.003), but not linked with IL‐6 (p = 0.096). In children with remission asthma, MALT1 only was negatively associated with Th1 cells (p = 0.023), but positively linked with TNF‐α (p = 0.023).ConclusionMucosa‐associated lymphoid tissue lymphoma translocation protein 1 serves as a potential biomarker for monitoring exacerbation risk and Th1/Th2 imbalance‐mediated inflammation of asthma children.
K48- and K27-mutant ubiquitin regulates adaptive immune response by affecting cross-presentation in bone marrow precursor cells
Authors: Jia Jun Jun , Liao Xiao Yan , Liang Yi Yun , Chen Rui Ling , Gao Feng Guang 
Published: March 2022

AbstractK48-linked ubiquitination determines antigen degradation and plays vital roles in the process of cross-presentation of bone marrow precursor cell (BMPC)-derived dendritic cells (DCs). Although previous studies revealed that K48 and K27-linked ubiquitination regulates innate immunity, the exact roles of K48 and K27-linked ubiquitination in cross-presentation and BMPC-based adaptive immunity are still uncertain. In this study, we investigated the effects of K48- and K27-mutant ubiquitin (Ub) on BMPC-based adaptive immune response by observing the effects of MG132, Ub deficiency, and K48/K27-mutant Ub on cross-presentation, T cell proliferation, IFN-γ secretion, BMPC-based CTL priming, and thereby the efficiency of cytolytic capacity of BMPC-activate T cells. We demonstrated that MG132, Ub deficiency, and K48-mutant Ub impair cross-presentation, T cell proliferation, IFN-γ secretion, BMPC-based CTL priming, and the cytolytic capacity of BMPC-activated T cells. Moreover, although K27-only Ub decreases cross-presentation, the replenishment of K27-mutant Ub restores Ub deficiency impaireds the abilities of T cell proliferation, IFN-γ secretion, CTL priming, and the cytolytic capacity of BMPC-activated T cells. Thus, these data suggest that K48- and K27-linked ubiquitination contributes to BMPC-mediated adaptive immune response by affecting BMPC cross-presentation and the cytolytic capacity by up-regulating both perforin and granzyme B in BMPC-activated T cells. K48- and K27-mutant Ub might have potential clinical therapeutic function in adaptive immune response-associated diseases.
Robust engraftment of fetal nonhuman primate CD34-positive cells in immune-deficient mice
Authors: Little Christopher J , Haynes William J , Huang Liupei , Daffada Cross M , Wolfe Bryce K , Perrin Elizabeth , Simpson John A , Kropp Schmidt Jenna A , Hinkle Hayly M , Keding Logan T , Behrens Ryan T , Evans David T , Kaufman Dixon B , Thomson James A , Golos Thaddeus G , Brown Matthew E 
Published: March 2022

AbstractNonhuman primates (NHPs) represent one of the most important models for preclinical studies of novel biomedical interventions. In contrast with small animal models, however, widespread utilization of NHPs is restricted by cost, logistics, and availability. Therefore, we sought to develop a translational primatized mouse model, akin to a humanized mouse, to allow for high-throughput in vivo experimentation leveraged to inform large animal immunology-based studies. We found that adult rhesus macaque mobilized blood (AMb) CD34+-enriched hematopoietic stem and progenitor cells (HSPCs) engrafted at low but persistent levels in immune-deficient mice harboring transgenes for human (NHP cross-reactive) GM-CSF and IL3, but did not in mice with wild-type murine cytokines lacking NHP cross-reactivity. To enhance engraftment, fetal liver-derived HSPCs were selected as the infusion product based on an increased CD34hi fraction compared with AMb and bone marrow. Coupled with cotransplantation of rhesus fetal thymic fragments beneath the mouse kidney capsule, fetal liver-derived HSPC infusion in cytokine-transgenic mice yielded robust multilineage lymphohematopoietic engraftment. The emergent immune system recapitulated that of the fetal monkey, with similar relative frequencies of lymphocyte, granulocyte, and monocyte subsets within the thymic, secondary lymphoid, and peripheral compartments. Importantly, while exhibiting a predominantly naïve phenotype, in vitro functional assays demonstrated robust cellular activation in response to nonspecific and allogenic stimuli. This primatized mouse represents a viable and translatable model for the study of hematopoietic stem cell physiology, immune development, and functional immunology in NHPs.Summary Sentence : Engraftment of rhesus macaque hematopoietic tissues in immune-deficient mice yields a robust BLT/NeoThy-type primatized mouse model for studying nonhuman primate hematopoiesis and immune function in vivo.
Effectively counseling the military population: Training needs for counselors
Authors: Arcuri‐Sanders Nicole M. , Forziat‐Pytel Kellie 
Published: March 2022

AbstractCounselors (N = 49) interested in working with the military population described what is needed to feel competent and confident counseling this population in a mixed‐methods research study. Findings suggest counselors do not believe their programs are preparing them to work with this population. Participants indicate strategies which would increase their ability to work with the military. Results provide implications for counseling programs on how they can better prepare students to work effectively with this population and increase the employment rates in serving the military.
Novice school counselors’ role in closing the achievement gap with low‐income adolescents
Authors: West Erin M. , Moate Randall M. , McKinney Robert T. 
Published: March 2022

AbstractWe explored 10 novice school counselors’ experiences working to close the achievement gap with low‐income adolescents through interpretative phenomenological analysis. Results suggest that school counselors experienced their role in closing the achievement gap with low‐income adolescents as one of (a) systemic discouragement, (b) interactions with students’ home environment, and (c) parallel process advocacy. Implications for school counselors, counselor educators, and future research are included.
Cancer Risk Associated with Continuous Positive Airway Pressure: A National Study
Authors: Brauer Philip R. , Bryson Paul C. , Wu Shannon S. , Gau Victoria L. , Lamarre Eric D. , Kominsky Alan H. 
Published: March 2022

ObjectiveIn 2021 the U.S. FDA issued a Class 1 safety recall notice for specific devices due to a risk of carcinogen exposure. The objective of this study was to evaluate reports of cancer linked to CPAP devices to understand implications for the field of sleep medicine.MethodsCases of cancer involving CPAP devices were retrieved from the MAUDE database from 2014 to 2021 and analyzed with descriptive statistics.ResultsA total of 2571 patient injuries were associated with CPAP. Reports of cancer (n = 209; 4.62%) were the second most commonly documented patient problem associated with CPAP, although 1950 (43.13%) patients had a device problem without an associated injury. Of the 209 cancer cases associated with CPAP, 200 (95.7%) of the adverse event reports were received by the FDA in 2021. There were 174 (9.15%) descriptions of the CPAP polyurethane sound abatement foam degrading in association with a cancer diagnosis, but degradation was more commonly not associated with malignancy (n = 1728; 90.85%). Other frequently documented CPAP device problems included broken devices (n = 279; 6.92%), fire (n = 182; 4.51%), and patient–device incompatibility (n = 144; 3.57%).ConclusionMalignancy associated with CPAP devices has been reported; however, future studies are required to establish causation. Given 95.7% of those documented cases were reported in 2021, otolaryngologists should be prepared to discuss the risks of carcinogenesis associated with CPAP. The otolaryngology community should also be aware of the potential bandwagon effect and the implications for CPAP compliance.Level of Evidence4 Laryngoscope, 132:2270–2274, 2022
Distinct stabilization characteristics of organic carbon in coastal salt‐affected soils with different salinity under straw return management
Authors: He Wei , Wang Hui , Ye Wenhua , Tian Yanling , Hu Guoqing , Lou Yanhong , Pan Hong , Yang Quangang , Zhuge Yuping 
Published: March 2022

AbstractStraw returning effectively promotes the soil organic carbon (SOC) pool, but the straw‐induced change in SOC quality (C fraction) remains unclear, particularly in saline soil. Here, we investigated how straw returning and soil salinity (low, medium, and high) affect SOC fractions, including easily oxidized and particulate organic C (EOC and POC) and mineral‐associated organic C (MOC) at aggregate scales, including macroaggregates (MA), microaggregates (MI), and silt + clay particles. The results revealed that SOC fraction contents in the whole soil decreased with increasing soil salinity, and straw returning increased the total organic C (TOC) in both low‐ and medium‐salinity soils. In low‐salinity soil, straw returning induced decreases of 12.1% and 30.6% in MI‐associated EOC and POC, respectively, and promoted significant increases of MI‐associated MOC, indicating that 'hierarchical aggregation' theory (i.e., single soil particles gradually form clusters, MI and MA) dominated during soil aggregate formation. In medium‐salinity soil, straw application increased all SOC fraction contents in MA, and raised the POC/TOC of the whole soil by 56.6%, suggesting that the 'macroaggregate turnover' theory (MI is formed within MA) played a more important role in forming aggregates. Our results demonstrated that low‐ and medium‐salinity soils had different C‐increasing strategies under straw returning: MA contributed more to the increased SOC than MI in low‐salinity soil, whereas the reverse was true in medium‐salinity soil. These findings suggest that higher soil salinity would weaken the C sequestration potential of straw returning, and that targeted measures should be applied to enhance reclamation efficiency.