bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of thrombus histopathology with mechanical thrombectomy: an in vitro study tested by thromboelastography and magnetic resonance imaging
Authors: Liu Xing-Long , Wang Bin , Zu Qing-Quan , Shi Hai-Bin , Liu Sheng 
Published: March 2022

Abstract
Objective: To detect the association between the content of red blood cell (RBC) within thrombi and thromboelastography, magnetic resonance imaging and retrieving strength during thrombectomy in vitro.Methods: Arterial and venous blood samples, considered as RBC-rich groups, were collected from twenty healthy beagle dogs under general anesthesia. RBCs were diminished by gathering the plasma and buffy coat layer after centrifugation. Then samples were grouped as: arterial whole blood group, venous whole blood group, arterial plasma group and venous plasma group. Blood routine test and coagulation test were used to quantify the content of RBC, platelet and fibrin. After tested by thromboelastography, these four groups of blood samples were proceed to clots and compared by magnetic resonance imaging (MRI), HE staining and mechanical thrombectomy in vitro. Results: TEG assay showed MA of whole blood groups with high RBCs was significantly higher than that of plasma groups. MRI demonstrated the plasma clots were much more homogeneous than whole blood samples. T2 value of plasma clots on GRE T2*MR imaging sequence were higher than that of whole blood groups (P<0.001). The whole blood clots showed significant higher tension, but were more fragile compared with that of plasma clots. Moreover, clot tension was found positively correlated with MA.Conclusion: RBC-rich clots showed lower T2 value, higher retrieving strength but more fragile during mechanical thrombectomy in vitro compared with those of plasma clots. Further, the retrieving strength was positively correlated with MA.
Identification of Potentially Invasive Wood Borers From China Using Sentinel Gardens of Stressed American Trees
Authors: Ernstsons A. Simon , Li You , Ji Yingchao , Wang Bo , Ruan Yongying , Hulcr Jiri 
Published: March 2022

Abstract
Thousands of insect species have been introduced to non-native regions around the world; many have harmlessly integrated into the new ecosystems, while some became agents of mortality of the new plant hosts. To prepare for possible new invasions, scientists and agencies need to identify traits that predispose wood boring species to become tree-killers, and focus on species that possess these traits. Here, we report results of field trials in three different sentinel gardens of North American trees in China, seeking to identify wood borers demonstrating aggressive traits while still in their native range. We also added flooding and partial girdling of the experimental trees to detect wood borers attracted to physiologically stressed but living trees. We identified two species of Cerambycidae (Anoplophora chinensis, Chlorophorus signaticollis) and 3 species of Curculionidae-Scolytinae (Cryphalus lipingensis, C. piceus, Hyledius kesiyae) colonizing living, North American oaks and pines. We have also documented additional seven borer species which colonized our trees only after their vitality index had dropped below recovery, a trait associated with secondary, non-damaging borers. Identifying potential pests and documenting their interactions with the host in sentinel gardens supports risk analysis, and enables trading partners to assess the potential impact of pest-host interactions prior to an invasion.
Expression of a copper activated xylanase in yeast: Differential properties of C-tagged or untagged recombinant xylanases
Authors: ELGHARBI Fatma , HLIMA Hajer BEN , HMIDA-SAYARI Aïda 
Published: March 2022

Abstract
Background: Endo-xylanase (EC 3.2.1.8), which hydrolyze the backbone structure of β-1,4-xylans, can be used together with laccase (activated by copper) to hydrolyse lignocellulosic and hemicellulosic materials in bioethanol production. A previous study described the cloning of the xylanase gene from Aspergillus niger US368 and obtaining the recombinant His-tagged protein using E. coli expression system. This recombinant xylanase was activated by copper which is a strong inhibitor for xylanases activities. The activation of the enzyme toward copper is due to the 36 extra amino acids provided by the vector pET28a. However, the expression of this xylanase was relatively low and intracellular that limits its potential industrial application. Hence, we aimed to over-express this interesting enzyme using the Pichia pastoris system for use in biofuel production.Results: A copper activated xylanase produced by E. coli BL21 was expressed in Pichia pastoris using the pGAPZαB expression vector. Two recombinant xylanase forms were obtained (Non-C-terminal tagged-His-tagged r-XAn11 and C-terminal tagged-His-tagged r-XAn11). The findings revealed that the two recombinant xylanases displayed different behaviors toward the copper. In the presence of 3 mM Cu2+, the relative activity of the Non-C-terminal tagged-His-tagged r-XAn11 was enhanced by about 52%. However, the xylanase activity of the C-terminal tagged one was strongly inhibited by copper. The Non-C-terminal tagged recombinant enzyme was more thermostable, in the presence of 3 mM Cu2+, than the C-terminal tagged one with a half-life of 10 min at 60 °C. 3D models of the two recombinant forms were constructed. The results showed that the created copper site in the Non-C terminal tagged protein was loosed in the C terminal tagged protein due to the high fluctuation and probably new interactions among the C and N-terminal amino acids.Conclusion: The present work reports the expression and purification of two recombinant xylanases forms (non C-terminal tagged and C-terminal tagged His-tagged r-XAn11).
Non-contact Intraocular Pressure Measurement over Bandage Contact Lens and the Effect of Pentacam and Corvis ST’s IOP Correction System
Authors: Cai Xueting , Qin Yi , Liu Sixiu , Cheng Zhewei , Lu Fan , Qu Jia , Chen Ding 
Published: March 2022

Abstract
Purpose: To evaluate the influence of intraocular pressure (IOP) measurement while wearing bandage contact lens (BCL) and the effect of Pentacam and Corvis ST’s correction systems. Methods: It was a prospective comparative study. Forty healthy subjects (Forty eyes) were included in this study. Eyes were measured by non-contact tonometer (NCT), Corvis ST and Pentacam before and after wearing BCL. Pentacam’s five correction formulas (Ehlers formula, Shah formula, Dresden formula, Kohlhaas formula, Orssengo/Pye formula) and Corvis ST’s correction formulas (Ehlers formula and biomechanical corrected formula) were used to correct the IOP values before and after BCL wearing. The IOP values were compared and the correction effect of different systems were evaluated.Results: The mean age of the subjects was 24.4±0.60 years. The mean IOP obtained by NCT was 14.8±3.2mmHg before BCL wearing, and was 15.7±3.4mmHg after BCL wearing. The mean IOP was significantly increased after BCL wearing (0.9±2.9mmHg, P=0.05). Four of the five Pentacam’s correction formulas (except Kohlhaas formula) showed no significant difference in the mean corrected IOP values before and after BCL wearing (all P>0.05). The mean IOP obtained by Corvis ST was 13.7±2.8mmHg before BCL wearing, and was 15.0±4.0mmHg after BCL wearing. The mean IOP was significantly increased after BCL wearing (1.3±2.4mmHg, P<0.05). Corvis ST’s correction formula (Ehlers formula other than biomechanical corrected formula) showed no significant difference in the mean corrected IOP values before and after BCL wearing (P>0.05).Conclusion: The IOP measurements over BCL by NCT and Corvis ST was found to be increased. The correction systems of Pentacam (Ehlers formula, Shah formula, Dresden formula, and Orssengo/Pye formula) and Corvis ST (Ehlers formula) are useful in correcting the IOP measuring deviation induced by BCL wearing.
LncRNA HCG11 suppresses the progression of breast cancer through cooperating with miR-330-3p/FOXO1 axis
Authors: Zhu Xiaotao , Wang Fan , Wang Mingzheng , Lv Lin , He Dan , Fan Chen 
Published: March 2022

Abstract
Background: Breast cancer is the most common cancer among women and the second most common cancer among newly diagnosed cancers worldwide. At present, long non-coding RNAs (lncRNAs) are widely reported to be involved in the occurrence and development of multiple cancers. As a newly discovered long non-coding RNA, the function of HLA complex group 11 (HCG11) remain uncertain in breast cancer. This article aims to examine the effect of HCG11 in breast cancer.Methods: RT-PCR was performed to detect the mRNA level of HCG11 in both breast cancer tissues and cell lines. HCG11 overexpression virus and siHCG11 were constructed and transfected to the breast cancer cell line MCF-7. The effect of HCG11 were evaluated by CCK-8 assay, colony formation assay, wound scratch assay, and cell invasion assay. Then we found and determined small RNA that may interact with HCG11 by bioinformatics analysis and luciferase report analysis. Next, we further researched the role of miR-330-3p in HCG11-mediated breast cancer by CCK-8 assay, colony formation assay, wound scratch assay, and cell invasion assay. At last, the above experimental methods and nude mouse experiments were used to confirm the role of HCG11/miR-330-3p/ FOXO1 in breast cancer process.Results: The results showed that the mRNA level of HCG11 was low-expressed in breast cancer tissues and cell lines. HCG11 overexpression inhibited cell proliferation, invasion and migration in MCF-7 cell line. Moreover, HCG11 could target miR-330-3p/FOXO1 and further suppressed the progression of breast cancer. Conclusion: In summary, lncRNA HCG11 suppressed the progression of breast cancer through cooperating with miR-330-3p/FOXO1 axis
ELIMINATOR: Essentiality anaLysIs using MultIsystem Networks And inTeger prOgRamming
Authors: Antoranz Asier , Ortiz Maria , Pey Jon 
Published: March 2022

Abstract
A gene is considered as essential when it is indispensable for cells to grow and replicate under a certain environment. However, gene essentiality is not a structural property but rather a contextual one, which depends on the specific biological conditions affecting the cell. This circumstantial essentiality of genes is what brings the attention of scientist since we can identify genes essential for cancer cells but not essential for healthy cells. This same contextuality makes their identification extremely challenging. Huge experimental efforts such as Project Achilles where the essentiality of thousands of genes is measured together with a plethora of molecular data (transcriptomics, copy number, mutations, etc.) in over one thousand cell lines can shed light on the causality behind the essentiality of a gene in a given environment. Here, we present an in-silico method for the identification of patient-specific essential genes using constraint-based modelling (CBM). Our method expands the ideas behind traditional CBM to accommodate multisystem networks. In essence, it first calculates the minimum number of non-expressed genes required to be active by the cell to sustain life as defined by a set of requirements; and second, it performs an exhaustive in-silico gene knockout to find those that lead to the need of activating additional non-expressed genes. We validated the proposed methodology using a set of 452 cancer cell lines derived from the Cancer Cell Line Encyclopedia where an exhaustive experimental large-scale gene knockout study using CRISPR (Achilles Project) evaluates the impact of each removal. We also show that the integration of different essentiality predictions per gene, what we called Essentiality Congruity Score, (derived from multiple pathways) reduces the number of false positives. Finally, we explored the gene essentiality predictions for a breast cancer patient dataset, and our results showed high concordance with previous publications. These findings suggest that identifying genes whose activity is fundamental to sustain cellular life in a patient-specific manner is feasible using in-silico methods. The patient-level gene essentiality predictions can pave the way for precision medicine by identifying potential drug targets whose deletion can induce death in tumour cells.
Green approach for the regioselective synthesis of β-thiol-1,4-disubstituted-1,2,3-triazoles using Fe3O4@SiO2-PMA-Cu as a novel and efficient magnetically recoverable nanocatalyst
Authors: Eisavi Ronak , Ahmadi Fereshteh 
Published: March 2022

Abstract
The magnetic nanoparticles of Fe3O4 were synthesized through a solid-state reaction of hydrated iron (III) chloride, hydrated iron (II) chloride and NaOH, and then purified by calcination at high temperature. To protect ferrite nanoparticles from oxidation and agglomeration, as well as to manufacture a novel catalytic system of anchored copper on the magnetic substrate, the Fe3O4 was core-shelled by adding tetraethyl orthosilicate. Next, the prepared Fe3O4@SiO2 was supported by phosphomolybdic acid (PMA) as the second layer of nanocomposite at 80 °C after 30 hours. Eventually, the new nanocomosite of Fe3O4@SiO2-PMA-Cu was successfully synthesized by adding copper (II) chloride solution and solid potassium borohydride. The structure of magnetic nanocatalyst was acknowledged through different techniques such as EDS, VSM, XRD, TEM, FT-IR, and FESEM. The synthesis of β-thiolo/benzyl-1,2,3-triazoles from various thiiranes, terminal alkynes and sodium azide was catalyzed by Fe3O4@SiO2-PMA-Cu nanocomposite in aqueous medium. In order to obtain the optimum condition, the effect of reaction time, temperature, catalyst amount and solvent was evaluated. The recycled catalyst was used for several consecutive runs without any loss of activity.
Modified and Alternative Baveno VI Criteria Based on Age for Ruling Out High-risk Varices in Patients With Compensated Cirrhosis
Authors: Zhao Lili , Wang Ting , Guo Chunxia , Zhou Li , Han Ping , Wang Chunyan , Ma Ying , Wang Jing , Gao Min , Li Jia 
Published: March 2022

Abstract
Background: The Baveno VI criteria(B6C) was recommended to screen high-risk varices(HRV) in liver cirrhosis patients so that esophagogastroduodenoscopy(EGD) could be safely avoided. It was perceived as conservative, and Transient elastography is not available in many medical institutions; this restricts the clinical application of B6C. We aimed to optimize the B6C to screen more patients who could safely avoid screening EGD and to explore the parameters to replace liver stiffness(LS).Methods: 1188 patients with compensated cirrhosis were analyzed and divided into the training cohort(TC) and validating cohort(VC) by the split-sample method. Variables were selected to construct the new criteria in the TC and were validated in the VC. Results: Age≥50 years, LS, platelet count(PLT), and spleen area (SA) were independently associated with HRV. The risk of HRV was 2.39 times higher in patients over 50 years, so the alternative B6C(AB6C) and modified B6C(MB6C) criteria were built based on age. MB6C was built by adjusting the cutoff value of LS and PLT (patients aged<50 years, the criteria of PLT>100×109/L and LS<30kPa; aged≥50 years, combination of PLT>125×109/L and LS<20 kPa). The predicting performance HRV showed no statistical difference between PLT, SA and LS. SA was selected to replace the LS and built AB6C(patients aged<50 years, the criteria of PLT>100×109/L and SA<55cm2; aged≥50 years, combination of PLT>125×109/L and SA<44 cm2). Conclusions: The excellent performance of MB6C and AB6C, which stratified by age, were validated for safely ruling out high-risk varices and these could help avoid more EGD procedures than B6C and EB6C.Clinical trial registration number: ChiCTR-DDD-17013845.
Evolutionary Relationships, Phylogeographic Patterns, and a Hypothesis for Air- and Sea-driven Global Spread of Chromoblastomycosis by Etiological Fonsecaea Agents
Authors: Wang huiWei , Hao Juan , Cai Liang-Qi , Chen Min , Fang Wen-Jie , Pan Bo , Zhang Chao , Liu Xiao-Gang , Lei Wen-Zhi , Du Lin , Zhang Lei , Cao Cun-Wei , Xue Xiao-Chun , Yang Ya-li , Chen Xiang-Fang , Zhao Ying , Li Xiao-Jing , Yang Ying , Ling Li-Yan , Jiang Wei-Wei , Pan Weihua , Chen Liu-qing , Liao Wan-Qing 
Published: March 2022

Abstract
Fonsecaea spp. is the most common pathogen underlying chromoblastomycosis. However, many aspects of its evolutionary history are not fully understood, and a timescale for its diversification is lacking . Here, we propose, explain, and validate the evolutionary relationships, transmission routes, and modes of transmission among pathogenic species of the genus Fonsecaea . we investigated the sequences of a total of 510 intra-ribosomal transcribed regions and 485 reported cases of eight strains of the genus Fonsecaea , that have spread epidemically worldwide, persisted, and diversified over 38.27 million years. This period coincides with the diversification of many filamentous fungi, and this evolution explains the diversity and origin of pathogenic species of Fonsecaea spp . , as well as the possible transmission routes. Fonsecaea pedrosoi originated in the Atlantic Ocean near Brazil, F. monophora originated in the Pacific Ocean near southern China, and F. nubica originated in the Indian Ocean near Madagascar. F. pugnacius originated and is endemic in the Atlantic Ocean near Brazil. We proposed the hypothesis that non-pathogenic environmental strains in the environment ( Fonsecaea brasiliensis , F. multimorphosa , F. minima , and F. erecta ) evolved into pathogenic strains ( F. pedrosoi , F. pugnacius , F. nubica , and F. monophora ) and spread globally with global tropical cyclone and ocean currents pathways. F. multimorphosa is potentially infectious to humans. The present study’s results will greatly facilitate the prevention, treatment, and cure of Fonsecaea infections and give insight and reference to researchers in the field of fungal research, especially other pathogenic fungi and environmental fungi.
Characteristics of a Radish Mutant with Longer Siliques
Authors: fu jun , yu xiaomin , li zhengrong , Jin Die , Zhu Jinjin , zhu yuxin , liu mingru , yin yanni , li shisheng 
Published: March 2022

Abstract
The silique is an important organ in plant reproduction and maintains biodiversity among species; however, little is known about the regulation of radish silique development. In this study, we conducted research on the radish long silique (ls) mutant and wild-type (WT) radish and compared their morphological and molecular markers. The results showed that the mutant obtained by ethyl methane sulfonate treatment had the following stable characteristics: the lengths of mutant and WT siliques were 20.50 and 9.10 cm, respectively; and the ovule number per silique of the mutant and WT was 9.5 and 4.5, respectively. Polymerase chain reaction (PCR) analysis revealed abundant polymorphisms between the ls mutant and WT in HZ001, SRC9-022, and OIL2F11 simple-sequence repeat molecular markers, and the expression of LS1 and LS2 (RsNAC66). Arabidopsis thaliana-transformed plants with RsNAC66 overexpression were obtained by the floral dip method. Quantitative PCR showed that LS2 (RsNAC66) was more highly expressed in transformed lines than in WT, and the expression of LS2 (RsNAC66) in the transformed lines was higher in siliques than leaves. Phenotypic analysis revealed abnormal ovule numbers and shoots, and altered plant height in the transformed plant. Phenotypic and gene expression analyses showed that LS2 (RsNAC66) had a positive effect on silique length and the number of seeds per silique. Together, the results showed that the radish ls mutant is specific and stable, and thus constitutes an excellent research resource for improving the seed yield of radishes.
Amine-functionalized magnetic biochars derived from invasive plants Alternanthera philoxeroides for enhanced efficient removal of Cr(VI): performance, kinetics and mechanism studies
Authors: Luo Xin , Du Haiying , Zhang Xiaochao , Yang Yuhang 
Published: March 2022

Abstract
In this study, novel magnetic biochars derived from Alternanthera philoxeroides and modified by different amines (Hexanediamine, Melamine and L-Glutathione) were successfully prepared and employed as an efficient adsorbent for Cr(VI). The maximum adsorption capacity is 80.58 mg/g obtained by Hexanediamine modified biochar (MFBAP), which is nearly twice that of the pristine biochar (42.47 mg/g). BET demonstrated that Hexanediamine and Melamine could enhance the SBET of biochars, while L-Glutathione could reduce its SBET, which SEM images could support this conclusion. Adsorption kinetics and isotherms studies showed that the Cr(VI) adsorption process of MFBAP followed Elovich kinetic model and Langmuir isotherm, which means that it was mainly a chemical adsorption process. The characterization results proved that -NH2 derived from amines play a significant role in removing Cr(VI), which is mainly degraded by complexation reaction, electrostatic interaction and reduction.. In sum, the biochar modified by amines has excellent Cr(VI) adsorption performance, highly enhanced SBET and excellent recyclability, which is a promising candidate for solving the problem of invasive plants and wastewater treatment.
Toward a BT.2020 green emitter through a combined multiple resonance effect and multi-lock strategy
Authors: Liu Junyuan , Zhu Yunhui , Tsuboi Taiju , Deng Chao , Lou Weiwei , Wang Dan , Liu Tiangeng , Zhang Qisheng 
Published: March 2022

Abstract
Color-saturated green-emitting molecules with high Commission Internationale de L'Eclairage (CIE) y values have great potential applications for displays and imaging. In this study, we linked the outer phenyl groups in multiple-resonance (MR)-type blue-emitting B (boron)-N (nitrogen) molecules through bonding and spiro-carbon bridges, which resulted in planar and rigid green emitters with thermally activated delayed fluorescence. The MR effect and multiple interlocking strategy greatly suppressed the high-frequency vibrations in the molecules, which emit green light with a full-width at half-maximum of 14 nm and a CIE y value of 0.77 in cyclohexane. These were the purest green molecules with quantum efficiency and color purity that were comparable to the best quantum dots currently available. Doping these emitters into a traditional green-emitting phosphorescence organic light-emitting diode (OLED) endowed the device with a Broadcast Service Television 2020 color-gamut, 50% improved external quantum efficiency, and an extremely high luminescence of 4.6×105 cd/m2, making it the greenest and brightest OLED ever reported.
Water quality analysis based on phytoplankton and metal indices: A case study in the Sauce Grande River Basin (Argentina)
Authors: Zunino Josefina , Colla Noelia S La , Brendel Andrea S , Alfonso Maria B , Botté Sandra E , Perillo Gerardo M E , Piccolo Maria C 
Published: March 2022

Abstract
This study presents the first integrated water quality assessment of the Sauce Grande River Basin. This South American freshwater environment contains two water bodies that interrupt its course before the river discharges into the ocean: a dam for water supply and a shallow lake. We analyzed the spatial distribution of the phytoplankton community, the physicochemical parameters, and the metal concentrations in the particulate fraction. According to the trophic indices and the phytoplankton abundance, composition and diversity, the water quality showed significant deterioration in the lower basin after the Sauce Grande lake. The conductivity, turbidity and most metal concentrations increased towards the downstream area, even exceeding recommended levels for some metals. This study generates a database for the water quality of the Sauce Grande River Basin and its contribution to the sea. It shows an example of how the water quality varies along a basin that crosses different topographic environments and land covers.