bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple classification for the endovascular recanalization of vertebral artery stump syndrome
Authors: Zhang Wenbin , Li Chao , Shi Mingchao , Zhou Jie , Yue Feixue , Song Kangjia , Wang Shouchun 
Published: August 2022

Abstract
Objective: To share our single-center experience in the treatment of vertebral artery stump syndrome (VASS) and assess the role of the comprehensive classification based on anatomic development, proximal conditions, and distal conditions (PAD).Methods: Data of patients who underwent endovascular thrombectomy (EVT) at Stroke Center of the First Hospital of Jilin University from January 2016 to December 2021 were retrospectively collected. Among patients with acute ischemic stroke in the posterior circulation, those with acute occlusion of the intracranial arteries in the presence of occlusion at the origin of the vertebral artery were selected after confirmation by digital subtraction angiography. Clinical data were summarized and analyzed.Results: We included 13 patients with VASS. The overall surgical recanalization success rate was 76.92%. The successful proximal recanalization rate was 66.67%; the recanalization rates for P1, P2, P3, and P4 types were 100%, 66.7%, 50%, and 66.7%, respectively. The average operation time for A1 and A2 types was 127.5 ± 37.03 (80–180) and 120 min, respectively. The successful distal recanalization rate was 90%—100%, 75%, 100%, and 100% for D1, D2, D3, and D4 types, respectively. Four patients had perioperative complications (incidence rate: 30.77%). Distal embolism occurred in three patients (incidence rate: 23.08%). No dissection or subarachnoid hemorrhage occurred in any patient.Conclusion: EVT is an effective and feasible treatment for VASS, and comprehensive PAD classification can, to a certain extent, help in initially determining the difficulty of surgery and providing guidance for the interventional procedures.
System-level analysis of flux regulation of yeast show that glycolytic flux is controlled by allosteric regulation and enzyme phosphorylation
Authors: Chen Min , Xie Tingting , Li Huan , Zhuang Ying-Ping , Xia Jianye , Nielsen Jens 
Published: August 2022

Abstract
Energy metabolism is central for cellular function and has therefore evolved to be tightly regulated such that energy production can be balanced to energy demand. Energy is being produced in the central carbon metabolism (CCM) and even though there has been extensive studies on how fluxes through the different pathways in this part of metabolism are regulated. There is little understanding of how fluxes are affected by posttranslational modifications and by allosteric regulators. Here we integrated multi-omics data (intracellular metabolome, extracellular metabolome, proteome, phosphoproteome, and fluxome) under 9 different chemostat conditions for building a mathematical model that could map functional regulatory events (FREs) in the Saccharomyces cerevisiae. Using hierarchical analysis combined with the mathematical model, we observed pathway and metabolism-specific flux regulation mechanisms in the CCM. We also found that the glycolytic flux increased with specific growth rate, and this increase was accompanied by a decrease of both metabolites derived FREs and protein phosphorylation level.
Meat Quality, Some Blood Profiles and Cecum Microbial Load in Broiler Fed With Effective Microorganisms, Turmeric (Curcuma longa) and Their Combination as Feed Additives
Authors: Wakjira Chala Kinati , Ameha Negasi , Girma Meseret , Nurfeta Ajebu 
Published: August 2022

Abstract
A study was conducted to evaluate effect of feeding effective microorganisms (EM), turmeric (TP) and their combination (EM-TP) as natural feed additives on meat quality, some blood profiles and cecum bacterial load of broilers. A total of 192 chicks were assigned into one of four treatments control (CTL), 1ml/lit EM, 1% TP, 0.5 ml/lit EM and 0.5% TP (EM-TP) in a completely randomized design. Feed additives had higher (P < 0.05) b*(yellowness) value in breast and thigh meat color for TP and EM-TP than the other treatments. Shear force value is higher (P < 0.05) in CTL than the other in both breast and thigh muscles. The highest (P < 0.05) fat content was for CTL. The moisture, CP and ash of breast and thigh meat were similar (P > 0.05) except crude fat percentage which was higher (P < 0.05) for CTL group. High result (P < 0.05) in alkaline phosphatase (ALP) for EM and EM-TP while Cholesterol content, Low-Density Lipoprotein (LDL), and Triglycerides concentrations were decreased significantly (P < 0.05) by consumption of EM, T, P, and EM-TP than the control group. There were high (P ≤ 0.05) population of total coliform count and E. coli bacteria were counted in CTL than the other treatment group. In conclusion, experimental additives can lower the crude fat in meat composition, blood cholesterol level, blood LDL cholesterol, triglyceride concentration and markedly reduced total coliform count and E. coli bacteria in the intestinal contents of broilers thereby improving gut efficiency and gut health of broiler chicken.
Association between outdoor air pollution and chronic rhinosinusitis patient outcomes
Authors: Peeters S. , Wang C. , Bijnens E.M. , Bullens D.M.A. , Fokkens W.J. , Bachert C. , Hellings P.W. , Nawrot T.S. , Seys S.F. 
Published: August 2022

Abstract
Background: The aetiology of chronic rhinosinusitis (CRS) is multifactorial with a complex interplay between environmental, microbial endogenous and genetic factors. The impact of outdoor air pollution on prevalence or severity of CRS remains largely unknown. Methods: Real-life geolocation data (2017-2018, Belgium) from 278 CRS patients (2576 health records) using the mySinusitisCoach mobile application were analysed to calculate the patients’ individual exposure to outdoor air pollutants (ozone (O3), black carbon (BC), nitrogen dioxide (NO2) and particulate matter with diameter <2.5 μm (PM2.5)) and associate these pollutants with the patients’ sinus related symptoms measured at multiple occasions by visual analogue scale (VAS).Results: The adjusted seasonal model for the spring-summer (n=1000 health entries, N=83 patients) population revealed an increase of 6.07 (p<0.0001) in overall CRS symptom scoring for an interquartile range (IQR) increase in exposure to O3 (26.9 μg/m3). An increase of 1.69 (p=0.05) in total CRS symptom scoring was observed for an IQR increase of PM2.5 (7.1 µg/m3) exposure. Gender-stratified analysis in the spring-summer population showed significant interaction between air pollution and gender with male patients having higher total CRS symptom scores for an IQR increase in exposure to PM2.5 (3.52, p=0.001), and O3 (8.33, p<0.0001), while no significant association with symptom severity was seen in the female patients. In the analysis stratified for comorbid asthma, CRS patients with comorbid asthma had higher total CRS symptoms for an IQR increase in exposure to PM2.5 (2.58, p=0.04) and O3 (7.72, p<0.0001) while the patients without comorbid asthma had no significant symptom increases. Conclusion: Exposure to outdoor air pollution is associated with increased symptom severity in CRS patients. The extent to which CRS patients are sensitive to outdoor air pollution exposure varies per season and depends on their gender and comorbid asthma status. mHealth technology has the potential to reveal novel insights on the patients’ exposome and disease severity in the real-life situation.
68Ga-Pentixafor PET/CT for Subtyping Diagnosis of Primary Aldosteronism: A Prospective, Diagnostic Accuracy Study
Authors: Hu Jinbo , Xu Tingting , Shen Hang , Song Ying , Yang Jun , Zhang Aipin , Ding Haoyuan , Xing Naiguo , Li Zhuoyuan , Qiu Lin , Ma Linqiang , Yang Yi , Feng Zhengping , Cheng Qingfeng , Wang Zhihong , Du Zhipeng , He Wenwen , Sun Yue , Li Qifu , Chen Yue , Yang Shumin 
Published: August 2022

Abstract
Purpose: Adrenal vein sampling (AVS) is the recommended procedure for subtyping primary aldosteronism (PA) as unilateral PA (UPA) or bilateral PA (BPA) which needs adrenalectomy and medication treatment, respectively. However, AVS is invasive and technically difficult. How to subtype PA non-invasively is currently a great challenge. We aimed to prospectively evaluate the accuracy of 68Ga-Pentixafor positron emission tomography/computed tomography (PET/CT) in subtyping PA, using AVS as a reference standard.Methods: Patients diagnosed with PA were recruited to undergo AVS and 68Ga-Pentixafor PET/CT. Maximum standardized uptake value (SUVmax) of each adrenal during the PET/CT was measured to calculate the lateralization index (LI) of SUVmax. Area under the receiver operating characteristic curve (AUC), specificity and sensitivity were used to analyze the accuracy of LI based on SUVmax for subtyping PA.Results: One hundred PA patients completed the study, including 43 UPA and 57 BPA. SUVmax of adrenals at 10min during the PET/CT positively correlated with aldosterone concentration (r=0.27, P=0.0001) as well as aldosterone-cortisol ratio (r=0.38, P<0.0001) in adrenal veins. Using LI based on SUVmax at 10min to identify UPA, the AUC was 0.90 [95% confidence interval (CI), 0.83-0.97]. Cutoff at 1.65 conferred a specificity of 1.00 (95%CI, 0.94-1.00) and sensitivity of 0.77 (95%CI 0.61-0.88). The diagnostic concordance rate of PET/CT and AVS was 90%, compared to 54% between traditional adrenal CT and AVS. An algorithm employing the LI based on SUVmax for subtyping resulted in a 62% reduction in the number of PA patients who needed AVS.Conclusions: 68Ga-Pentixafor PET/CT is a promising subtyping method for PA with high diagnostic accuracy. For a large proportion of PA patients, 68Ga-Pentixafor PET/CT could be used to avoid invasive AVS.Trial registration number：ClinicalTrials.gov: NCT 05131061. Registered 10 November 2021.
Assessment of antibody responses against SARS-CoV-2 in unvaccinated individuals and vaccinees from Omicron-BA.2 infection in Zhaoqing, Guangdong Province, China
Authors: Zhang Huan , Jiang Yushan , Tan Hanqing , Zou Lirong , Zheng Zhonghua , Huang Yushi , Lin Shujian , Su Lebin , Yu Jianxiang , Deng Xiaoling , He Jianfeng , Liu Chang , Shen Chenguang , Li Baisheng 
Published: August 2022

Abstract
Currently, the majority of the global population has been vaccinated with the COVID-19 vaccine, and characterization studies of antibodies in vivo from Omicron breakthrough infection and naive infection populations are urgently needed to provide pivotal clues about accurate diagnosis, treatment, and next-generation vaccine design against SARS-CoV-2 infection. We showed that after infection with Omicron-BA.2, the antibody levels of specific IgM against the Wuhan strain and specific IgG against Omicron were not significantly elevated within 27 days of onset, Interestingly, in this study, the levels of humoral immunity against Omicron specific IgM were significantly increased after breakthrough infection, suggesting that the detection of Omicron specific IgM antibodies can be used as a test criterion of Omicron breakthrough infection. In addition, we observed that serums from unvaccinated individuals and the majority of vaccinated infections possessed only low or no neutralizing activity against Omicron at the onset of Omicron breakthrough infections, and at the later stage of Omicron-BA.2 breakthrough infection, levels of neutralization antibody against the Wuhan and Omicron strains were elevated in infected individuals. The findings of this study provide important clues for the diagnosis of Omicron breakthrough infections, antibody characterization studies, and the design of next-generation vaccines for COVID-19.
A dynamical traffic flow model for a cognitive drivers' sensitivity in Lagrangian scope
Authors: Hossain Md. Anowar , Tanimoto Jun 
Published: August 2022

Abstract
A new microscopic traffic flow model is established based on heterogeneous driver's sensitivity; in this new model, the driver's sensitivity is defined as being dependent on the headway distances to the preceding vehicle, as in the optimal velocity (OV) function. We introduce the formulation of this driver's sensitivity using a different form of the OV function. Using the linear stability condition, we elucidate a neutral stability condition. A series of numerical simulations indicates how the present model describes dynamics that differ from the conventional model, which assumes a constant driver's sensitivity.
Laboratory and preclinical evaluation of a novel wound dressing patch for treatment of wounds in the elderly
Authors: Arjunan Subramanian , Teo Nelson , Claudine Stephanie , Tam Kimberley , Feng Pan , Lim Jane , Biswas Arijit , Bongso Ariff , Fong Chui Yee , Choolani Mahesh 
Published: August 2022

Abstract
Background
Elderly patients develop pressure ulcers that are very difficult to treat and current treatments have several limitations. Native skin stem cells diminish with age and may explain poor skin renewal in the elderly. Hence, an exogenous supply of stem cells to wounds in the elderly may produce a successful therapy. We evaluated the wound healing potential of an aloe vera-polycaprolactone nanocarrier impregnated with human Wharton’s jelly stem cells (hWJSCs + AV/PCL) or its conditioned medium (hWJSC-CM + AV/PCL) on elderly human skin using in vitro wound assays and on excisonal wounds created in an elderly preclinical mouse model.
Methods
hWJSCs and human skin fibroblasts (HSFs) were derived and characterized using our previously published protocols. The influence of hWJSC-CM on elderly human skin fibroblasts (eHSFs) were compared with that of young HSFs (yHSFs) and untreated eHSF controls. The healing of excisonal wounds created in elderly mice over a 21 day period was evaluated using various methods.
Results
Scratch wounds of hWJSC-CM-treated eHSFs completely closed by day 2 compared to untreated eHSF controls. Collagen and elastin levels were significantly increased while senescence-related genes were significantly downregulated in hWJSC-CM-treated eHSFs compared to untreated eHSFs. Angiogenesis assays produced significantly greater tubule numbers and ring formation in the presence of CM from hWJSC-CM-treated eHSFs. Excisional wounds in elderly mice treated with hWJSC + AV/PCL and hWJSC-CM + AV/PCL completely healed by day 21 and wound closure rates were significantly greater compared to controls. Histology of elderly mice wounds treated with hWJSC + AV/PCL and hWJSC-CM + AV/PCL showed changes in skin structure. Epidermal and dermal thickness, CD31 and gene expression levels of ECM, collagen, angiogenesis, scarless, granulation and immune cell chemoattraction were significantly greater in elderly mice treated with hWJSC + AV/PCL and hWJSC-CM + AV/PCL.
Conclusions
The results confirmed that hWJSCs in combination with the stem cell niches in nanoscaffolds and the antimicrobial properties of aloe vera provide an attractive wound dressing patch for treatment of chronic wounds in the elderly.
Complex dielectric behaviours in BiFeO3/Bi2Fe4O9 ceramics
Authors: Orr Gilad , Gorychev Andrey , Ishai Paul Ben 
Published: August 2022

Abstract
The complex dielectric permittivity of a sintered ceramic tablet consisting of 70.5% BiFeO3 and 27.7% Bi2Fe4O9 was analyzed as a function of temperature from − 120°C to 230°C in two separate temperature runs. The results reveal a complicated dielectric response with 2 temperature activated relaxation processes. The first is purely Arrhenius relaxation related to hopping processes between Fe3+ and Fe2+ traps induced by oxygen vacancies at grain boundaries. The second process is more unusual, having a critical temperature point at T0 = 766 K and disappearing during the second temperature run. It can be related to dynamic rearrangements of domain boundaries between different crystallites in the ceramic. The results also reveal a ferroelectric phase transition that decayed with repeated heating cycles of the tablet. The ferroelectric phase transition in pure BiFeO3 is 1098 K, whereas the current results show it at 373 K. The origin of this reduction in the critical temperature of the phase transition is traced to locally induced strains on grain boundaries because of unit cell size mismatch between BiFeO3 and Bi2Fe4O9.
Patterns and Drivers of Internal Migration and Its Association with Access to Reproductive and Maternity Care Among Women in Pakistan: Evidence from a Recent National Survey
Authors: Dadras Omid , Nakayama Takeo 
Published: August 2022

Abstract
Background
During the last decades, migratory behavior has had a key role in population growth and redistribution in Pakistan. Migration has far-reaching socioeconomic implications for individuals and society at large that could influence the health integrity of Pakistani women. This study aimed to describe the migration patterns and drivers as well as its association with adequate access to reproductive and maternal care among married Pakistani women aged 15–49.
Methods
The data from the 2017-18 Pakistan Demographic Health Survey (PDHS) was used to extract the information on the explanatory (sociodemographic and migration backgrounds) and outcome variables (unmet needs for family planning, adequate antenatal care, and delivery at health facilities). Bivariate and multivariate logistic regression analyses were employed to examine the relationship between these explanatory and outcome variables before and after adjustment for sociodemographic inequalities.
Results
In unadjusted models, the odds of having adequate ANC and delivery at health facilities were approximately 2 to 4 times higher in those living (urban non-migrant), moving to (urban to urban, rural to urban), or leaving the urban areas (rural to urban) as compared to rural non-migrants; likewise, the odds of the unmet needs for family planning was about 20–50% lower in the same migration streams compared to rural non-migrant. However, after adjustment for sociodemographic inequalities, most of these associations attenuated and only the association of urban to urban migration with unmet needs for family planning and the association of urban non-migrant with delivery at health facilities remained significant.
Conclusion
Although the findings suggest that Internal migration flows, particularly those to urban areas (urban to urban and rural to urban), could be associated with better access to reproductive and maternity care among married Pakistani women aged 15–49 years; adjustment for sociodemographic inequalities, particularly education and wealth, nullified this association to a great extent. This has important implications for current policies and interventions in Pakistan and calls for policy reform and women’s rights advocacy to enhance the literacy level of young Pakistani girls through well-tailored interventions, maintaining them at school.
uPAR is regulated via miR-561-3p and affects the progression and aggressiveness of CRC cells via the PI3K/AKT signaling pathway
Authors: Yang Xi , Wang Xuelian , Sang Lu 
Published: August 2022

Abstract
Urokinase plasminogen activator (uPA), the uPA receptor (uPAR) and plasminogen activator inhibitor type 1, constitute the plasminogen urokinase activation system that is hypothesized to be associated with the malignant biology of cancer cells. However, the regulation mechanism of uPAR expression in colorectal cancer (CRC) remains unclear. In the present study it was demonstrated that uPAR was upregulated in serum samples from patients with CRC and uPAR expression levels were associated with advanced stages of CRC and distant metastasis in patients. In CRC cells, uPAR was demonstrated to promote cell proliferation, migration and invasion, but inhibited cell apoptosis. Furthermore, the results demonstrated that uPAR expression was negatively regulated via miR-561-3p, via the binding of miR-561-3p to its 3’-untranslated region. Moreover, uPAR reduced the activation of the PI3K/AKT signaling pathway. In conclusion, uPAR was downregulated via miR-561-3p and potentially contributed to tumorigenesis via the PI3K/AKT signaling pathway in CRC.
Electrographic lead I and V5 monitoring could have detected a missed left-side pneumothorax intraoperatively.
Authors: Lee Chihjen , Yumul Roya , Vongchaichinsri Colby , Tsai Kevin , Wang Lena 
Published: August 2022

Abstract
We present an EKG monitoring strategy to detect pneumothorax during high-risk surgery. In the literature, EKG changes and pneumothorax are well described. However, anesthesiologists only monitor lead II on a three-lead EKG system in the operating room. In our case, there was only a subtle change in lead II for a left-sided pneumothorax, which could have been easily missed. On the other hand, there was a marked QRS amplitude reduction and T wave flattening/inversion in lead I and V5. We recommend lead V5 be added to the continuous monitoring and lead I be periodically checked for surgeries known to potentially cause pneumothorax.
Underlying mechanism for “loss of passivation” effect of a high-carbon martensitic stainless steel coating via laser cladding
Authors: Lu Siyuan , Gao Shurui , Liu Hensan , Fang Xufei , Lu Wenjun , Li Shuxin , Chen Yunbo 
Published: August 2022

Abstract
The microstructure and pitting performance of high-carbon martensitic stainless steel (HMSS) samples, which were produced via laser cladding (LC) and hot isostatic pressing (HIP) were comparatively investigated via electrochemical measurements and electron microscopies. Dendritic and network connected M23C6 carbides are the main precipitates in the HMSS coating, while the M23C6 carbides in HMSS bulk are spherical or elongated in shape. Pitting resistance of the HMSS coating is dramatically deteriorated. The Cr-depleted region induced by the Cr-enriched and dendritic M23C6 carbides are considered as underlying factor for the “LOP” effect of the HMSS coating.