bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA Sequencing Analysis of Peripheral Blood Mononuclear Cells Reveals a Novel Pathway Associated with COVID-19 Deterioration
Authors: Ikemachi Mami , Ogura Rina , Miyagawa Kazuya 
Published: July 2022

Abstract
The severity of COVID-19 depends on the host immune cell response. Most patients with COVID-19 had mild disease, but up to 30% of patients developed a severe disease. Risk factors associated with severe diseases such as age, smoking, and comorbidities are known, while molecular signatures that predict the disease progression are still emerging. Here, we investigated gene expression profiles in peripheral blood mononuclear cells (PBMCs) from patients with COVID-19 using RNA-sequencing analysis (RNA-seq) based on the hypothesis that deteriorating patients have a unique molecular signature in PBMCs at an early stage of the disease. Twelve patients were included in RNA-seq (deteriorated vs. nondeteriorated, each n = 6). RNA-seq demonstrated 156 differentially expressed genes, and pathway analysis showed that “Nucleosome assembly” was the most important pathway, with 24 HIST1 cluster genes upregulated in the deteriorated group. In silico prediction of upstream transcription factors exhibited protein inhibitors of activated STAT 4 (PIAS4) as the most significant. TP53, STAT1, and IKBKG associated with COVID-19 pathophysiology are predicted to interact with PIAS4. Our results show that elevated HIST1 cluster gene expression predicts COVID-19 deterioration, which may induce histone-mediated lung injury and coagulopathy. The molecular mechanism could involve PIAS4-mediated transcriptional regulation and inflammatory signal transduction.
LIN28B Promotes Cancer Progression by Regulating the Warburg Effect via PFKP in Epithelial Ovarian Cancer
Authors: Wang Simin , Wu Yong , Guo Xueqi , Guo Qinhao , Chen Siyu , Wu Jiangchun , Ha Xinyu , Wen Hao , Wu Xiaohua 
Published: July 2022

Abstract
Background: Ovarian cancer (OC) is one of the most aggressive and fatal malignancies in women, especially high-grade serous ovarian cancer, which has a mortality rate of 90% and a five-year survival rate of only 31%. LIN28B is an evolutionarily RNA-binding protein that is aberrantly activated in cancer, leading to the dysregulation of posttranscriptional gene expression. However, the possible roles and mechanisms of LIN28B in OC have not been fully explored.Results: Expression of LIN28B was upregulated in ovarian cancer and significantly correlated with poor prognosis. Cox analysis revealed that high expression of LIN28B was an independent predictor of overall survival among late-stage OC patients. Functional experiments demonstrated that LIN28B overexpression promoted ovarian cancer cell proliferation and invasion, while knockout did the opposite. Xenograft tumour model indicated that overexpressing LIN28B promoted tumour growth in mice. Metabolic analysis revealed that LIN28B modulated mitochondrial respiration and glycolytic processes. Mechanistically, LIN28B bound and stabilized PFKP mRNA to facilitate the Warburg effect, rescue functional and metabolic assays further confirmed this phenomenon.Conclusions: Our study reveals a novel pathway independent of the LIN28/let-7 axis by which LIN28B promotes ovarian cancer progression by stabilizing PFKP mRNA to mediate the Warburg effect. LIN28B may be used as a prognostic predictor and a potential therapeutic target in ovarian cancer.
Complementary use of multi-model climate ensemble and Bayesian Model Averaging for projecting river hydrology in the Himalaya
Authors: Ahsan Shafkat , Bhat M. Sultan , Alam Akhtar , Farooq Hakim , Shiekh Hilal A. 
Published: July 2022

Abstract
This study aims to predictthe hydrology of the Jhelum basin under changing climatic scenarios based on Representative Concentration Pathways (RCPs). Six Global Circulation Models were dynamically downscaled for reliable climatic projections over the 21st century (2020–2080). To reduce the uncertainty associated with climate projections, multi-model ensemble estimations were refined using Bayesian Model Averaging (BMA). The assessment reveals that compared tothe baseline (1980–2010) values, the annual mean maximum temperature in the basin will rise by 0.41–2.31°C and 0.63–4.82°C and the mean minimum temperature will increase by 1.39–2.37°C and 2.14–4.34°C under RCP4.5 and RCP8.5respectively. While as, precipitation is expected to decreases by 7.2–4.57% and 4.75–2.4 7% under RCP4.5 and RCP8.5 correspondingly. BMA ensemble projections were used inthe Soil and Water Assessment Tool (SWAT)to simulate the future hydrological scenarios of the drainage basin. With the changing climate, the discharge of rivers in the Jhelum basin is expected to witness reductions by about 23–37% for RCP4.5 and 19–46% for RCP8.5. Moreover, the water yield of the basin may also exhibit decreases of 17–25% for RCP4.5 and 18–42% for RCP8.5. The projected scenarios are likely to cause water stress, affect the availability of water for diverse uses, and trigger transboundary water sharing related conflicts. The impact of climate change on discharge demands early attention for the development of mitigation and adaptive measures at the regional level and beyond.
Comparison between conventional and compressed sensing cine cardiovascular magnetic resonance for left atrial volume and strain assessment
Authors: Chen Yang , Xu Panpan , Liu Wangyan , Zhu Yinsu , Zhou Xiaoyue , Xu Yi , Zhu Xiaomei 
Published: July 2022

Abstract
Purpose: To investigate the feasibility of compressed sensing (CS) cine in quantifying left atrial (LA) volumes and strain assessments compared with conventional segmented cine.Methods: Segmented and CS cine sequences were acquired in 31 patients with LV diastolic dysfunction defined by echocardiography (21 males, age 51±15 years) and healthy volunteers (22 males, age 39±13 years) using an 3T MR scanner. LA volumes and strains were evaluated in both sequences. Results: There was excellent correlation for normalized LA volumes (ICCs ≥ 0.982), good correlation for LA EFs (ICCs ≥ 0.779) and moderate correlation for LA strains (ICCs ≥ 0.535) for both cines. Compared with segmented cine technique, LA passive EF from CS cine was not statistically different (segmented: 26.2 (10.7, 32.5) vs. CS: 24.9 (11.7, 35.4), p = 0.838), but radial and longitudinal strain derived by CS cine technique were markedly underestimated (all p < 0.001). The LA passive EF (EFpassive), passive radial and longitudinal strain values (Ere and Ele) from both cine techniques showed good diagnostic performance without significant differences in discriminating between patients and healthy controls (EFpassive, p=0.794; Ere, p=0.513; Ele, p=0.346).Conclusion: Compared with segmented cine, LA EFs obtained from CS cine were clinically comparable and LA strain parameters were underestimated. However, the performance of two cine methods to discriminate between patients with and without LV diastolic dysfunction is similar.
The right of access to healthcare: An analysis of how legal and institutional frameworks constrain or facilitate access to healthcare for residents in border areas in the East African Community
Authors: Ssengooba Freddie , Babirye Susan , Tuhebwe Doreen , Ssennyonjo Aloysius , Ssendagire Steven , Rutaroh Arthur , Mutesa Leon , Nangami Mabel 
Published: July 2022

Abstract
Background: Despite many countries working hard to attain Universal Health Coverage (UHC) and the Health-related Sustainable Development Goals, access to healthcare services has remained a challenge for communities residing along national borders in the East Africa Community (EAC). Unlike the communities in the interior, those along national borders are more likely to face access barriers and exclusion due to low health investments and inter-state rules for non-citizens. This study explored the legal and institutional frameworks that facilitate or constrain access to healthcare services for communities residing along the national borders in EAC. Methods: This study is part of a broader research implemented in East Africa (2018-2020), employing mixed methods. For this paper, we report data from a literature review, key informant interviews and sub-national dialogues with officials involved in planning and implementing health and migration services in EAC. The documents reviewed included regional and national treaties, conventions, policies and access rules, regulations and guidelines that affect border crossing and access to healthcare services. These were retrieved from official online and physical libraries and archives. Results: Overall, the existing laws, policies and guidelines at all levels do not explicitly deal with cross border healthcare access especially for border residents, but address citizen rights and entitlements including health within national frameworks. There is no clarity on whether these rights can be enjoyed beyond one’s country. The review found examples of regionalization of health infrastructure and strategies to invest in shared specialized services (centers of excellence) although this was not addressing border resident communities. The regional EAC policies entail opportunities for increased collaboration and integration between EAC States. In practice, disease surveillance and epidemic control are the main formal collaborative undertakings among sub-national officials responsible for health and migration services. Tools like health insurance cards, national identification cards and travel documents were found to influence access to health services across the borders in EAC.Conclusion: In the era of UHC, there is need to take advantage of the EAC integration to revise legal and policy frameworks to leverage existing investments and facilitate cross-border access to healthcare services for communities residing along EAC borders.
Periodical propagation of torsion in polymer gels
Authors: Yamada Yuhei , Otsuka Yuji , Mao Zebing , Maeda Shingo 
Published: July 2022

Abstract
Gel actuators have potential in soft robotics. Although there are many gel actuators that realize various motion like contraction, expansion, and bending motions, most of those reported to date require external inputs such as batteries and circuits. Herein we propose a periodical torsional motion hydrogel driven by chemical energy from the Belousov-Zhabotinsky (BZ) reaction. Our BZ gel system realizes autonomous motion without a battery. The elastic moduli of the redox states of the BZ gel are investigated using stress-strain analysis. We design an experimental system with integrating the BZ gel and two PDMS (dimethylpolysiloxane) rotators to evaluate the torsion angles and build a model of the rotary motion. Finally, the experimental pre-twist angle dependence of the rotary motion is compared with the theoretical model. The results should contribute to the development of soft actuators without external components.
Comparison of Different Correction Formulas and Measurement Methods for the Accurate Determination of Intraocular Pressure after SMILE and FS-LASIK Surgery
Authors: Yang Zhiqing , Miao Na , Wang Lixiang , Ma Ke 
Published: July 2022

Abstract
Background To compare the accuracy of non-contact tonometry, Pentacam, and corneal visualization Scheimpflug technology (Corvis ST) for the measurement of intraocular pressure (IOP) after small incision lenticule extraction (SMILE) or femtosecond laser-assisted in situ keratomileusis (FS-LASIK) surgery.Methods A total of 49 patients (98 eyes) undergoing FS-LASIK or SMILE surgery at West China Hospital, Sichuan University from January to March 2021 were enrolled in this prospective, comparative, self-controlled study. IOP values were measured with non-contact tonometer, Pentacam, and Corvis ST before surgery and 1 month after surgery. Pentacam-derived postoperative IOP values were corrected using five correction formulas (Ehlers, Shah, Dresden, Orssengo-Pye, and Kohlhaas), while Corvis ST-derived values were corrected using a single formula. Results In the SMILE group, no significant differences were observed between the IOP values corrected with the Shah formula before and after surgery (t = 0.17, P = 0.869), whereas postoperative IOP values corrected with the other formulas were significantly different from the corresponding preoperative measurements (P < 0.05). In the FS-LASIK group, postoperative IOP values corrected with the Ehlers, Shah, or Corvis ST formulas were significantly different from the corresponding preoperative IOP measurements (P < 0.05), but no significant differences were observed between pre- and postoperative IOP values corrected with the Dresden (t = −0.08, P = 0.941), Orssengo-Pye (t = −0.52, P = 0.604), or Kohlhaas (t = 1.22, P = 0.231) formulas. Conclusions Pentacam’s Shah correction formula seemed to be the most appropriate method for accurately measuring postoperative IOP in patients undergoing SMILE surgery, while the Dresden, Orssengo-Pye, and Kohlhaas correction formulas of Pentacam were identified as the most reliable methods for estimating IOP in patients after FS-LASIK surgery.
Mechanism of residual stress superposition in milling process of thin-walled parts and comprehensive deformation control method
Authors: Shi Zhemin , Li Beizhi , Sun Zhihong 
Published: July 2022

Abstract
The processing deformation of thin-walled parts is difficult to control under the traditional process method because of the small rigidity of the parts and the large amount of removal. Simulation research of various process parameter combinations and engineering test are carried out for the typical thin-walled parts with 2mm wall thickness and diameter-thickness ratio more than 850 to solve the problem of deformation. Based on the umbrella-shaped special tool of YG8 tungsten steel, the influence of process parameters on the important indexes and distribution law of residual stress of thin-walled structure parts were studied. Residual stress superposition mechanism of process parameter combinations of semi-precision milling and fine milling are revealed. A process design method considering the deformation control objective of thin-walled parts is proposed. The method includes the principle of high-speed machining, the design criterion of process allowance considering the location deviation, and the optimization design method of process parameters and their combinations based on the high-speed and allowance allocation criterion, in order to improve residual stress and its distribution, reduce cutting deformation and the long-term stability of the thin-walled structure. The relative cross-section size, thickness, roundness and concentricity of thin-walled parts with conical surface were measured by roundness meter and CMM. Result reveals that the use of high-speed milling and the optimized combination of process parameters can effectively control residual stress and deformation.
Network Intrusion Detection via Tri-Broad Learning System based on Spatial-temporal Granularity
Authors: Li Jieling , Zhang Hao , Liu Zhihuang , Liu Yanhua 
Published: July 2022

Abstract
Network intrusion detection system plays a crucial role in protecting the integrity and availability of sensitive assets. Network traffic data contains a large amount of time, space, and statistical information. Existing research lacks the utilization of spatial-temporal multi-granularity data features and the mutual support among different data features, thus making it difficult to specifically and accurately identify anomalies. Taking into account the distinctions among different granularities, we propose a framework called Tri-Broad Learning System (TBLS), which can learn and integrate the three granular features. In order to accurately explore the spatial-temporal connotation of the traffic information, a feature dataset containing three granularities is constructed according to the characteristics of time, space, and data content. In this way, we use broad learning basic units to extract abstract features of different granularities, and then express these features in different feature spaces to enhance them separately. We use the “He” instead of the original initialization method in BLS to initialize the weights of feature nodes and enhancement nodes to achieve better detection accuracy. We exhibit the merits of our proposed model on the UNSW-NB15, CIC-IDS-2017, and mixed traffic datasets. Experimental JOURNAL OF SUPERCOMPUTING results show that TBLS outperforms the typical BLS in terms of various evaluation metrics and time consumption. Compared with other machine learning methods, TBLS achieves better performance metrics.
3D printed cellular structural composites with continuous fiber reinforcement: A deep study on printing structural parameters and bending performance
Authors: Cui Ziying , Huang Xiayan , Panahi-Sarmad Mahyar , Dong Ke , Xiao Xueliang 
Published: July 2022

Abstract
Herein, an upgraded fused deposition modeling (FDM) in a 3D printer was used to fabricate continuous fiber-reinforced composites (CFRCs) in various shape-filled cellular structures with Kevlar fiber and polylactic acid (PLA) as the reinforcement and matrix. The fruitful influences on the bending performance are printing path, fiber orientation, infill density, cell length, and layer thickness which have been systematically studied. A Finite Element (FE) model was established to compare with the experimental results. A three-point bending test revealed that, as the effective density increased, there was an undeniable rise in strength and stiffness of CFRCs with different infill structures. The structural parameters like cell length are greatly responsible for strengthening bending performance; this effect was evaluated by adjusting the fiber orientations along the stretching direction. The sample of the rhombus-filled cellular CFRCs with a layer thickness of 0.1 mm was found to have the highest bending strength and modulus of 53.61 MPa and 2728.41 MPa, respectively. Since the mechanical properties mostly depend on the core shape, cell length, and layer thickness, plenty of infill structures should be designed by a 3D printer to achieve the required bending strength and flexural modulus — this work is able to be a paradigm guide to reduce the number of printed samples.
Multistep synthesis, reactivity and X-ray structure of the anisole-terminated iron(II) polyhalogenoclathrochelates and their monoribbed-functionalized macrobicyclic derivatives
Authors: Limarev Ilya P. , Zelinskii Genrikh E. , Mosov Danila O. , Vologzhanina Anna V. , Dorovatovskii Pavel V. , Lazarenko Vladimir A. , Lebed Ekaterina G. , Voloshin Yan Z. 
Published: July 2022

Abstract
Multistep synthetic pathway towards a series of the anisoleboron-capped ribbed-functionalized iron(II) cage complexes was developed. Their hexachloroclathrochelate precursor was obtained by the template condensation of three dichloroglyoximate chelating ligand synthons with two molecules of 4-methoxyphenylboronic acid as a Lewis-acidic cross-linking agent on the iron(II) ion as a matrix. It easily underwent a stepwise nucleophilic substitution with S2- and O2-dinucleophilic aliphatic (ethanedithiolate) or aromatic (pyrocatecholate) agents, forming the stable X2 (X = S or O)-six-membered ribbed substituent(s) at a quasiaromatic cage framework. Performing of these reactions under the different reaction conditions (i.e., at various hexachloroclathrochelate-to-nucleophile molar ratios, a wide range of temperatures and a series of the solvents) allowed to control a predominant formation of its mono-, di- or triribbed-substituted macrobicyclic derivatives. Thus obtained iron(II) di- and tetrachloroclathrochelates can undergo their post-synthetic transformations with active nucleophilic agents. The latter complexes underwent a further nucleophilic substitution with the anionic derivative of n-butanthiol, thus giving the hexasulfide macrobicyclic compound with two functionalizing n-alkyl substituents in one of its three chelate a-dioximate fragments and two apical biorelevant anisole substituents. The obtained iron(II) clathrochelates, possessing a low-spin electronic d6 configuration, were characterized using elemental analysis, MALDI-TOF mass spectrometry, UV-vis, 1H and 13C{1H} NMR spectroscopies, and by the single-crystal X-ray diffraction experiments for the hexachloroclathrochelate precursor, its dichlorotetrasulfide macrobicyclic derivative and the monoribbed-functionalized hexasulfide cage complex. In all their molecules, the encapsulated iron(II) ion is situated in the centre of its FeN6-coordination polyhedron, the geometry of which is intermediate between a trigonal prism and a trigonal antiprism with the distortion angles φ from 21.4 to 23.4°. Halogen bonding between the polyhalogenoclathrochelate molecules in their crystals is observed.
Selective Extraction of Gold from Strong Acid E-waste Leachate Through H+-Triggered Cascade Reaction of Thiolated β-Cyclodextrin
Authors: Yang Liming , Fang Difang , Yang Guang , Shao Penghui , Hua Zan , Bi Feihu , Wei Hanchen , Tu Yunyun , Chen Changli , Luo Xubiao 
Published: July 2022

Abstract
Recycling precious metals from the increasing amount of electronic waste (e-waste) is of great significance for the sustainability of strategic metal resources. However, there are rarely reports of technologies that can efficiently and highly selectively recover precious metals from strong acid e-waste leachates. Herein, we demonstrated a simply synthesized thiolated β-cyclodextrin (T-CD) that can recover gold with high selectivity from strong acidic medium. The material has an exceptional adsorption capacity, approaching 1890.98 mg gold/g of adsorbent, and a superior selectivity coefficient of about 5×104 for gold from multi-metal coexistence solutions at pH 2. Sulfhydryl groups, as the adsorption sites, are capable of selectively enriching gold to T-CD surfaces. Simultaneously, the acetal of T-CD can be hydrolyzed into hemiacetal by the initiation of H+ in acidic solution with a more energetically favorable reaction barrier (TS = -3.38 eV) than that in neutral solution (TS = 5.36 eV). Further, the serial transformation process of carbon structure and gold valence state (Au3+ to Au) induced by redox reaction of acetal carbon were confirmed by XPS and DFT. In addition, T-CD shows a remarkable ability to extract gold directly from actual complex waste printed circuit board leachate, which facilitates the recycling of the acidic solution up to five times. The concept of H+-assisted enhancement of adsorbent performance provides a new guidance for the direct recovery of rare and precious metals from strongly acidic actual wastewater.