bluuebunny/crossref_metadata_2025_split huggingface dataset:
PROmotion of COvid-19 VA(X)ccination in the Emergency Department – PROCOVAXED: Study Protocol for a Cluster Randomized Controlled Trial
Authors: Rodriguez Robert , O’Laughlin Kelli , Eucker Stephanie , Chang Anna Marie , Rising Kristin , Nichol Graham , Pauley Alena , Kanzaria Hemal , Gentsch Alexzandra , Li Cindy , Duber Herbie , Butler Jonathan , Eswaran Vidya , Glidden Dave 
Published: March 2022

Abstract
Background
We conducted in-depth interviews to characterize reasons for COVID-19 vaccine hesitancy in emergency department (ED) patients and developed messaging platforms that may address their concerns. In this trial we seek to determine whether provision of these COVID-19 vaccine messaging platforms in EDs will be associated with greater COVID-19 vaccine acceptance and uptake in unvaccinated ED patients.
Methods
This is a cluster-randomized controlled trial (RCT) evaluating our COVID-19 vaccine messaging platforms in seven hospital EDs (mix of academic, community, and safety-net EDs) in four US cities. Within each study site, we randomized 30 one-week periods to the intervention and 30 one-week periods to the control. Adult patients who have not received a COVID-19 vaccine are eligible with these exclusions: 1) major trauma, intoxication, altered mental status, or critical illness; 2) incarceration; 3) psychiatric chief complaint; and 4) suspicion of acute COVID-19 illness. Participants receive an orally administered Intake survey. During intervention weeks participants then receive three COVID-19 vaccine messaging platforms (4-minute video, one-page informational flyer and a brief, scripted face-to-face message delivered by ED physicians and nurses); patients enrolled during non-intervention weeks do not receive these platforms. Approximately an hour after intake surveys, participants receive a Vaccine Acceptance survey during which the primary outcome of acceptance of the COVID-19 vaccine in the ED is ascertained. The other primary outcome of receipt of a COVID-19 vaccine within 32 days is ascertained by electronic health record review and phone follow-up. To determine whether provision of vaccine messaging platforms is associated with a 7% increase in vaccine acceptance and uptake, we will need to enroll 1290 patients.
Discussion
Highlighting the difficulties of trial implementation during the COVID-19 pandemic in acute care settings, our novel trial will lay the groundwork for delivery of public health interventions to vulnerable populations whose only health care access occurs in EDs.
Trial Status:
We began enrollment in December 2021 and expect to continue through 2022.
Conclusions
Toward addressing vaccine hesitancy in vulnerable populations who seek care in EDs, our cluster-RCT will determine whether implementation of vaccine messaging platforms is associated with greater COVID-19 vaccine acceptance and uptake in unvaccinated ED patients.
Does the Rubella Immunoglobulin G affect the severity of COVID- 19? Rubella Immunoglobulin G and COVID- 19
Authors: Sahebi Leyla , Hosseini Mohsen , Abdollahi Alireza , Farrokhzad Nahid , Ghazi Samrand Fattah , Noroozi Farzaneh Samaei , Ghiasvand Fereshteh 
Published: March 2022

Abstract
Objective &Aim: The coronavirus disease, so far (COVID-19) has brought about millions of infections and fatalities throughout the world. Our aim was to determine whether rubella IGG titers are inversely correlated with severity COVID-19 patients.Materials & Methods: This study was conducted among COVID-19 confirmed patients over 18 years of age. The disease severity levels were categorized by WHO interim guidance. The rubella-specific IgG antibody-titer spectrum was measured (within first 48 hours of hospitalization) by enzyme-linked immunosorbent assay (ELISA). Result: in a study of 46 inpatients with varying COVID-19 disease severity (mild, moderate, severe, and critical), we observed a negative correlation between rubella IgG antibody titers and COVID‐19 severity (P-Value=0.03), There was an interaction between COVID-19 vaccination history and rubella IGG on severity COVID-19 (P-Value=0.0015). There was an interaction between age group under 44 years (including national measles- rubella (MR) vaccination in Iran) and rubella IGG titers on severity COVID-19 too (p-value=0.014).Conclusion: In conclusion, MR vaccination seems to have a positive effect in reducing the severity of the disease, emphasizing that, the important and separate effect of the IGG rubella (due to natural or extrinsic immunity) titers is determining.
The Application Of Remodeled Glove Puppetry For Children With Developmental Disabilities: A Randomized Trial
Authors: Lai Chia Ching , Hsieh Hsieh-Chun , Chen Hsin-Yung , Lin Chien-Heng 
Published: March 2022

Abstract
Objective: To study the improvement of hand performance and play behavior in children with developmental disabilities using remodeled glove puppetry. Method: A pretest-posttest design was used in this randomized controlled trial to evaluate the intervention. Sixty-two children with developmental disabilities were randomly divided into experimental and control groups (n=31 each). Both groups underwent puppet play sessions once daily in playrooms. The children in the experimental group underwent rehabilitation by playing with the remodeled glove puppetry for 12 weeks, while the children in the control group played with non-remodeled glove puppetry. The Chinese puppet was remodeled using the Lego EV3® robot. We analyzed the motion kinematics of the hand using Siliconcoach® Pro 7 software and measured the force produced by the Baseline® hydraulic pinch gauge. The revised Knox Preschool Play Scale was used to score the play behavior. Results: The significant differences in posttest outcomes between control and experimental groups were hand kinematics (wrist ROM, F = 6.91, p = 0.011; MP ROM, F = 6.66, p = 0.012; PIP ROM, F = 13.16, p = 0.001) and play scales (space management, F = 8.98, p = 0.004; material management, F = 10.04, p = 0.002; pretense-symbolic, F = 9.39, p = 0.003; participation, F = 25.17, p = 0.000). Significant improvement was observed in most of the kinematics and play scores (p < 0.05) after rehabilitation with remodeled glove puppetry.Conclusion: Further studies using remodeled glove puppetry to improve the range of motion, material management and participation of play quality are useful and needed.
NUMB attenuates post-traumatic osteoarthritis by inhibitingBTRC and inactivating NF-κB pathway
Authors: Lv Zhou , Zhou Fubo , Cheng Xianqiang 
Published: March 2022

Abstract
Background
Post-traumatic osteoarthritis (PTOA) is closely related to the inflammatory response caused by mechanical injury, leading to joint degeneration. Herein, we aim to evaluate the role and the underlying mechanism of NUMB in PTOA progression.
Methods
Anterior cruciate ligament transection (ACLT)-induced rats and LPS-treated chondrocytes were used as in vivo and in vitro models of PTOA, respectively. NUMB overexpression plasmid (pcDNA-NUMB) were administered by intra-articular injection in PTOA model rats, and safranin O-fast green staining, the Osteoarthritis Research Society International (OARSI) Scoring System, and HE staining were used to evaluate the severity of cartilage damage. The secretion of inflammatory cytokines (TNF-α, IL-1β, IL-6) and chondrocyte-specific markers (MMP13, COL2A1) was detected by ELISA. Cell viability and apoptosis were evaluated by MTT assay and TUNEL staining.
Results
The expression of NUMB was lower expressed in ACLT-induced PTOA rats and LPS-treated chondrocytes. NUMB overexpression enhanced cell viability and reduced cell apoptosis, inflammation and cartilage degradation in chondrocytes stimulated by IL-1β. NUMB bound with BTRC to inhibiting p-IκBα expression, resulting in NF-κB pathway inactivation. BTRC overexpression reversed the promoting effect of NUMB overexpression on cell viability and the inhibitory effects of NUMB overexpression on apoptosis, inflammation and cartilage degradation in LPS-induced chondrocytes. Besides, overexpression of NUMB alleviated articular cartilage damage by repression of inflammation and cartilage degradation in ACLT-induced PTOA rats.
Conclusion
Our data indicated that NUMB negatively regulates BTRC regulated PTOA progression by BTRC/NF-κB pathway, which may be a viable therapeutic target in PTOA.
Efficacy and safety of de-escalation of denosumab for giant cell tumor of the bone
Authors: Nakata Eiji , Fujiwara Tomohiro , Kunisada Toshiyuki , Katayama Haruyoshi , Kondo Haruna , Sato Kohei , Kondo Hiroya , Takihira Shota , Ozaki Toshifumi 
Published: March 2022

Abstract
We aimed to investigate the efficacy and safety of denosumab de-escalation for giant cell tumors of the bone (GCTB). We retrospectively reviewed the medical records of nine patients with GCTB that were either unresectable or resectable, but not those of candidates for resection, who received de-escalated denosumab treatment at our institution between 2014 and 2021. The denosumab treatment interval was gradually extended to every 8 weeks, 12 weeks, and 24 weeks. We assessed the radiographic changes and clinical symptoms during the course of standard and de-escalated denosumab therapy. Denosumab interval was de-escalated after a median of 12 months of a standard 4-weekly treatment. Imaging showed that a good therapeutic response obtained with the 4-weekly treatment was sustained until the 8-weekly and 12-weekly treatment. According to the MD Anderson criteria, GCTB treated with de-escalated denosumab therapy resulted in a complete response in one patient and a partial response in eight patients, which were obtained with standard treatment. During the 24-weekly treatment, two patients remained stable, while one patient developed local recurrence. The extraskeletal mass reduced significantly with standard treatment, while tumor reduction was sustained during de-escalated treatment. The clinical symptoms significantly improved with standard treatment and remained improved during de-escalated treatment. In conclusion, de-escalated treatment of denosumab has clinical benefit as a maintenance treatment in patients with unresectable GCTB.
Impact of Nutrition Counselling on Multiple Sclerosis Patients Nutritional Status: Randomized Controlled Clinical Trial
Authors: Afifi Zeinab , Hassan Amr , Abdelrahman Nebal , Sayed Asmaa El , Salem Marwa 
Published: March 2022

Abstract
Background: Faulty dietary habits and overnutrition are prevalent among Egyptian multiple sclerosis (MS) patients who do not receive nutrition care as part of treatment. This study was conducted to explore the impact of nutrition counselling on the nutritional status of MS patients. Methods: A single-blinded, parallel randomized controlled clinical trial (RCT) was conducted at Kasr Alainy MS Unit (KASMU) on 120 Eligible MS patients from September 2019 to February 2020. Patients were randomly allocated to either Nutrition counseling intervention group (IG) or control group (CG). Allocation concealment was done by sequentially numbered opaque sealed envelopes. All patients were assessed initially then followed the KASMU standard management protocol for the study period. Only patients in the IG underwent initial teaching sessions followed by monthly evaluation (nutrition counseling). All patients were assessed at the end of end of 3 month follow up period. Sociodemographic data was gathered through a structured interview. Nutritional status was assessed anthropometrically, by 24-hour recall and by Malnutrition Universal Screening Tool (MUST). The 2 groups were compared initially and at the end of follow-up. Analysis of covariance (ANCOVA) was used to analyze the difference between the two arms in quantitative outcomes. Chi-squared or Fisher's exact tests, and Mann-Whitney U test were used in quantitative outcomes. The alpha value (α) was set at 5%. Both Intention To Treat (INT) and Perprotocol (PP) analyses were performed. Results: At baseline assessment, the prevalence of overweight and obesity was 31.7% and 32.5% respectively, mean body mass index (BMI) was 27.7 ± 5.7 kg/m2. The mean waist circumference was 93.5±11.9 and 99.2±13.1 cm for males and females, respectively. About 27.3% of males and 83.9% of females showed abdominal obesity. After 3 months of counseling, weight, BMI, waist circumference, food consumption and nutrient intake and adequacy significantly improved in the IG (p<0.05).Conclusion: Nutrition counselling significantly improved anthropometric measurements, food consumption, nutrient intake and adequacy. The study was registered on ClinicalTrial.gov and was given a code (NCT04217564).
Pericardial Computed Tomography Imaging Findings In The Setting Of Coccidioidomycosis
Authors: Madani Mohammad H. , Ghasemiesfe Ahmadreza , Abdelhafez Yasser G. , Nardo Lorenzo 
Published: March 2022

Abstract
Background: Pericardial disease can be a manifestation of infection and imaging can have a role in its diagnosis. Coccidiomycosis endemic fungal infection has been more frequently reported over the past few decades. Other than case reports or series, there has been no systemic study evaluating pericardial imaging findings in patients with coccidioidomycosis to the best of our knowledge. The purpose of this study was to evaluate for intrathoracic computed tomographic (CT) imaging abnormalities in patients with coccidioidal infection with specific emphasis on the pericardium.Methods: Retrospective review of radiology reports and clinical chart review was performed to identify patients with coccidiomycosis between January 2000 and September 2021 at our medical center. Diagnosis of infection was confirmed predominately with serology. Patients were excluded if a CT was not performed within 3 months of confirmed diagnosis date and if there was concomitant additional granulomatous or fungal infection. Chest computed tomography was reviewed for pericardial and additional intrathoracic findings.Results: The final retrospective cohort consisted of 37 patients. Imaging findings included lung nodules (N=37/37), consolidation (N=25/37), mediastinal or hilar lymphadenopathy (N=20/37) and pleural effusions (N=13/37). Eleven of 37 patients (30%) had either trace pericardial fluid (N=3/37) or small pericardial effusions (N=8/37). One patient had pericardial enhancement/thickening and history of pericardial tamponade. No other patient had clinically pericarditis or pericardial tamponade. Pericardial calcifications were not seen in any patient. Pericardial effusion was statistically associated with presence of pleural effusion as 9/13 patients with pleural effusion had pericardial effusion versus 2/26 patients without pleural effusion had pericardial effusion (p<0.001). Otherwise patients with and without pericardial imaging findings were similar in terms of demographics, comorbidities and other imaging findings. Conclusion: Although pulmonary parenchymal pathology is a common manifestation of coccidioidal infection, most patients with coccidiomycosis do not have pericardial imaging abnormalities on CT.
On-demand autonomous shape memory polymer
Authors: Xie Tao , Ni Chujun , Chen Di , Deng Shihong , Jin Xiuming , Shen Youqing , Zhao Qian 
Published: March 2022

Abstract
Stimuli-responsive shape-shifting polymers1-3 have shown unique appeals in emerging applications including soft robotics4-7, medical devices8, aerospace structures9, and flexible electronics10. Their externally triggered shape-shifting behavior offers on-demand controllability essential for many device applications. Ironically, accessing external stimulation (e.g. heating or light) under realistic scenarios has become the biggest bottleneck for increasingly demanding applications such as implantable medical devices8. In contrast, autonomous shape-shifting polymers11,12 can operate in a trigger-free manner, but at the cost of losing the ability for active manipulation. Combining the attractive yet conflicting attributes (on-demand and autonomous) in one material is highly desirable. Here, we achieve this seemingly unrealistic goal with a 4D printable shape memory hydrogel that operates via spinodal decomposition, with its shape-shifting dominated by internal mass diffusion instead of thermal conduction for common shape memory polymers8-10,13. This allows the hydrogel to undergo fully autonomous shape transformation, critically with an on-demand programmable onset. The precisely controlled shape-shifting behavior without requiring any external stimulation markedly lowers the barrier for future device implementation.
Comparison of preoxygenation efficiency measured by the oxygen reserve index between high-flow nasal oxygenation and facemask ventilation: A randomized controlled trial
Authors: Park Sujung , Kim So Yeon , Kim Min-Soo , Park Wyun Kon , Byon Hyo-Jin , Kim Hyun Joo 
Published: March 2022

Abstract
BackgroundThe novel high-flow nasal oxygenation is gaining popularity for preoxygenation. The oxygen reserve index (ORI), which is a non-invasive and innovative modality that reflects the arterial oxygen content, are recently introduced in general anesthesia. In our study, we compared the preoxygenation efficiency of high-flow nasal oxygenation and facemask ventilation using the ORI in adult patients undergoing general anesthetic induction for non-emergent surgery.MethodsThis single-center, two-group, randomized controlled trial included 197 patients aged ≥20 years who underwent orotracheal intubation for general anesthesia for elective surgery. The patients were randomly allocated to receive preoxygenation via facemask ventilation or high-flow nasal oxygenation. The ORI was monitored continuously during anesthetic induction period until the endotracheal intubation was completed, and compared between the two groups. The primary outcome was the highest ORI value achieved by oxygenation. The secondary outcome was the time required to reach the highest ORI observed during the induction of anesthesia.ResultsThe ORI increased during preoxygenation in all patients and tended to decrease from the injection of the neuromuscular blockers to the completion of intubation. At 1 minute of preoxygenation, the ORI was significantly higher in the high-flow nasal oxygenation group (0.34 ± 0.33) than in the facemask ventilation group (0.21 ± 0.28; P = 0.003). The highest ORI was not significantly different between the two groups (0.68 ± 0.25 in the high-flow nasal oxygenation group vs. 0.70 ± 0.28 in the facemask ventilation group; P = 0.505). The time required to reach the highest ORI value was not significantly different between the two groups (3.1 ± 2.2 minutes in the high-flow nasal oxygenation group and 3.6 ± 2.2 minutes in the facemask ventilation group; P = 0.113).ConclusionsHigh-flow nasal oxygenation results in an ORI-measured oxygenation state similar to that provided by facemask ventilation during the general anesthesia induction. We conclude that high-flow nasal oxygenation is a feasible and alternative preoxygenation technique for facemask ventilation.Trial RegistrationThis study was registered at clinicaltrials.gov (registration number: NCT04291339, registration date: 02/03/2020)
Are Active Video Games Useful In The Development Of Fundamental Movement Skill Of Non-Typically Developing Children?A Meta-Analysis
Authors: Li Sen , Song Yang , Cai Zhidong , Zhang Qingwen 
Published: March 2022

Abstract
Background
Active video games are increasingly used in sports rehabilitation, especially among children and adolescents. However, due to the variety of video games, the different intervention environments and intervention doses, there are often differences in treatment effects. There may be questions about the actual effectiveness of video games in sports rehabilitation. This study systematically evaluated the effect of active video games on the development of basic motor skills in children and adolescents with atypical development.
Methods
The literature in seven Chinese and English databases was retrieved from randomized controlled trials. The included studies were quantitatively evaluated using the PEDro Scale. Relevant data were then input and analyzed using Review Manager version 5.4.
Results
Seventeen papers were included. In the three subordinate concepts of fundamental movement skill, Active Video Games could significantly improve locomotor skill(standardized mean difference = 0.59, 95% confidence interval 0.38–0.80, P < 0.0001) and stability skill(standardized mean difference = 0.59, 95% confidence interval 0.17–1.00, P = 0.006) in non-typically developing children.But there was no significant difference compared with the control group(standardized mean difference = 0.11, 95% confidence interval − 0.49–0.72, P = 0.72) in object control skill.
Conclusions
The study shows that Active Video Games can improve locomotor skill and stability skill in non-typically developing children, but the effect on object control skill is uncertain, and more high-quality literature needs to be included in the future.
Development of the Genotyping Marker for Reeves’ Turtle (Mauremys Reevesii) Using High-resolution Melting (HRM) Analysis
Authors: Park Chang-Deuk , Kim Keun-Sik , Kim Keun-Yong , Heo Jung Soo , Oh Hong-Shik , Park Seon-Mi , Yoon Ju-Duk 
Published: March 2022

Abstract
The Reeves’ turtle, Mauremys reevesii, resides in freshwater bodies of Korea, China, Japan, and Taiwan, and is designated as an endangered species in the Republic of Korea (ROK). However, international pet trade among East Asian countries has made it difficult to identify the indigenous population of M. reevesii in the ROK. In this study, a genotyping marker targeting the mitochondrial cytochrome b (COB) region that easily distinguishes the Korean population was developed for high-resolution melting (HRM) analysis and applied to 27 blood samples obtained from diverse Korean freshwater bodies and pet trade markets. The HRM analysis produced three distinct HRM curves that were designated as the Korean Type, Chinese Type, and Minor Type, whose results exactly matched those of COB sequencing. Our genotyping method will be useful for the rapid and accurate identification of the native population of M. reevesii in the ROK.
First Report of F1534C kdr Mutation in Deltamethrin Resistant Aedes Albopictus From Northern Part of West Bengal, India
Authors: Modak Manas Pratim , Saha Dhiraj 
Published: March 2022

Abstract
West Bengal is a dengue endemic state of India with several dengue outbreaks in the recent years. Synthetic pyrethroids are the major arsenal used for the control of these vectors. The emergence of pyrethroid resistance in mosquitoes has recently been described from many parts of the world due to kdr (knock down resistance) mutations in the voltage gated sodium channel gene (vgsc). In Aedes albopictus, at least four such mutations had already been found. In this study, wild populations of Ae. albopictus were sampled from different locations of northern part of West Bengal, India. World Health Organization, 2016 recommended adult bioassay was followed throughout the experiment in order to determine susceptibility status. A total of 200 Ae. albopictus specimens including both phenotypically resistant and susceptible individuals were successfully amplified for F1534C kdr genotyping. Among them, 81% were homozygote susceptible,12.5% were heterozygote and 6.5% were homozygote resistant. Presence of F1534C mutation in pyrethroid resistant Ae. albopictus mosquitoes is the first report from India. Moreover, another novel mutation T1520I was also found to be coexisting with F1534C mutation in Ae. albopictus. This study will aid in identifying insecticide resistance mechanism and therefore, will reduce errors in vector control measures.