bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved method for surgical induction of chronic hypertension in mice
Authors: Skaria Tom , Aboouf Mostafa A. , Vogel Johannes 
Published: July 2022

ABSTRACT
Chronic hypertension can be induced in mice by one-kidney one-clip (1K1C) or two-kidney one-clip surgery, transgenic overexpression of angiotensinogen and renin, administration of deoxycorticosterone acetate-salt, supplying Nitro-L-arginine methyl-ester in the drinking water and Angiotensin-II infusion. Although each model has its own pros and cons, selection of a model that mimics human hypertensive disease accurately is essential to ensure rigor and reproducibility in hypertension research. 1K1C mice represent an efficient, budget-friendly, and translationally capable model; however, their use in preclinical research has remained largely hindered due to concerns about potential technical complexity and lack of reported information regarding procedure-related mortality rates. Here, we describe in detail an improved version of the 1K1C surgery in mice that has zero intraoperative mortality and excellent survival rates in a long-term setting and permits the development of stable chronic hypertension and its target organ complications. Key to this outcome is unilateral nephrectomy 1 week after renal artery clipping to decelerate the blood pressure (BP) increase, which allows the organism to adapt better to the BP rise. The technical and animal welfare improvements presented here may promote the acceptance of the 1K1C model.
Bioinspired Total Synthesis and Structure-Activity Relationship Studies on Aplaminal
Authors: Ohyoshi Takayuki , Zhao Yiwen , Akemoto Kei , Ishihara Takuma , Taniguchi Ayaka , Zhang Menghua , Kigoshi Hideo 
Published: July 2022

Abstract
The total synthesis of aplaminal having a unique triazabicyclo[3.2.1]octane skeleton was accomplished using biomimetic oxidative cyclization as a key reaction. This total synthesis enabled us to prepare aplaminal analogs with a variety of substituents at the para-position in the aromatic ring. We found that an electron-donating group at the para-position enhances cytotoxicity and a hydrogen bond donor at the para-position is suitable for cytotoxicity.
Evidence for yeast artificial synthesis in SARS-CoV-2 and SARS-CoV-1 genomic sequences
Authors: Lisewski Andreas Martin 
Published: July 2022

Background: Knowledge about the origin of SARS-CoV-2 is necessary for both a biological and epidemiological understanding of the COVID-19 pandemic. Evidence suggests that a proximal evolutionary ancestor of SARS-CoV-2 belongs to the bat coronavirus family. However, as further evidence for a direct zoonosis remains limited, alternative modes of SARS-CoV-2 biogenesis should also be considered. Results: Here we show that the genomes of SARS-CoV-2 and SARS-CoV-1 significantly diverge from other SARS-like coronaviruses through short chromosomal sequences from the yeast S. cerevisiae at focal positions that are known to be critical for host cell invasion, virus replication, and host immune response. For SARS-CoV-1, we identify two sites: one at the start of the RNA dependent RNA polymerase gene, and the other at the start of the spike protein’s receptor binding domain; for SARS-CoV-2, one at the start of the viral replicase domain, and the other toward the end of the spike gene past its domain junction. At this junction, we detect a highly specific stretch of yeast origin covering the critical furin cleavage site insert PRRA, which has not been seen in other lineage b betacoronaviruses. As yeast is not a natural host for this virus family, we propose an artificial synthesis model for viral constructs in yeast cells based on co-transformation of virus DNA plasmids carrying yeast selectable genetic markers followed by intra-chromosomal homologous recombination through gene conversion. Highly differential yeast sequence patterns congruent with chromosomes harboring specific auxotrophic markers further support yeast artificial synthesis. Conclusions: These results provide evidence that the genomes of SARS-CoV-1 and SARS-CoV-2 contain sequence information that points to their artificial synthesis in genetically modified yeast cells. Our data specifically allow the identification of the yeast S. cerevisiae as a potential recombination donor for the critical furin cleavage site in SARS-CoV-2.
Morphological inflectional rules for Karelian Proper verbs
Authors: Krizhanovskaya Natalia , Novak Irina , Krizhanovsky Andrew , Pellinen Nataliya 
Published: July 2022

A methodology for the development and implementation of inflectional rules for verbs in Karelian Proper is presented. The materials for this study were lemmas and word forms from the Open corpus of Veps and Karelian languages (VepKar) and the electronic version of the Karelian language Dictionary. The system of rules for automatic verb inflection for the Karelian Proper supradialect is of both practical and theoretical scientific interest. The new rules have already enabled entering 141 000 Karelian Proper word forms in the VepKar dictionary. The new program for word form generation has significantly reduced the time for adding the full inflectional paradigm of any Karelian Proper verb to the VepKar dictionary. One only needs to fill in several template parameters instead of 125 word forms.
Kokkuvõte. Natalia Krizhanovskaya, Irina Novak, Andrew Krizhanovsky, Nataliya Pellinen: Morfoloogilised muutereeglid päriskarjala verbide jaoks. Artiklis esitletakse metodoloogiat, mida kasutati muutereeglite väljatöötamisel ja rakendamisel päriskarjala verbide jaoks. Materjali moodustasid vepsa ja karjala keele avatud korpusest (VepKar) ning karjala keele sõnaraamatu elektroonilisest versioonist kogutud lemmad ja sõnavormid. Esmakordselt arendati välja reeglite süsteem päriskarjala verbivormide automaatseks genereerimiseks. See on teaduslikult huvipakkuv nii praktilise kui ka teoreetilise poole pealt. Uued reeglid on juba võimaldanud lisada 141 000 päriskarjala sõnavormi VepKar sõnaraamatusse. Uus sõnavormide genereerimise programm on oluliselt vähendanud aega, mis kulub täieliku muuteparadigma lisamisele mingi päriskarjala verbi juurde Vepkar sõnaraamatus. 125 sõnavormi asemel on selleks üksnes vaja täita mallid mõningate parameetritega.
Articulatory properties of Estonian palatalization by Russian L1 speakers
Authors: Malmi Anton , Lippus Pärtel , Meister Einar 
Published: July 2022

We studied the articulatory and temporal properties of Estonian palatalization by Russian L1 Estonian L2 speakers and compared the results to Estonian native speakers. The tongue movement of 24 Russian L1 and 21 Estonian L1 speakers were recorded with an electromagnetic articulograph. The speakers articulated phonologically contrastive word pairs and i-stemmed nouns where the final consonant was palatalized. Previous research has shown that palatalization is generally realized by raising the tongue dorsum towards the hard palate. Consequently, the tongue becomes more anterior and wider. This rising of the tongue also lengthens the duration of the segments. The results showed that Russian L1 speakers’ tongue dorsum was higher and more anterior when palatalizing consonants and the subsequent vowels. Compared to native speakers, their tongue was retracted and lower. Russian L1 speakers’ duration of palatalized consonants and the vowels that preceded them were significantly shorter than in the control group. They did not use duration to differentiate palatalization in word pairs as native speakers do.
Kokkuvõte. Anton Malmi, Pärtel Lippus, Einar Meister: Vene emakeelega kõnelejate Eesti palatalisatsiooni artikulatoorsed ja temporaalsed tunnused. Artiklis uuritakse, kuidas vene emakeelega kõnelejad hääldavad eesti keele palatalisatsiooni. Varasemad uurimused on näidanud, et konsonanti palataliseeritakse keeleselja tõstmisega kõva suulae poole. Sellega kaasneb ka eespoolsem keele asend ja laiem keele jaotus suulaes ning hääliku pikem kestus. Uurimismaterjal koosnes sõnadest, kus palatalisatsioon eristas tähendust ja i-tüvelistest nimisõnadest, mis lõppesid pika palataliseeritud konsonandiga. Testsõnu sisaldavaid lauseid salvestasime elektromagnetartikulograafiga. Katses osales 24 vene emakeelega ja 21 eesti emakeelega kõnelejat. Tulemused näitasid, et vene emakeelega kõnelejate keel oli palataliseerimisele omaselt kõrgemal ja eespoolsem. Eestlastega võrreldes oli nende keel aga madalamal ja tagapoolsem. Vene emakeelega kõnelejad ei kasutanud palatalisatsioonipaarides kestust eristava tunnusena nagu emakeelsed kõnelejad seda teevad. Nende vokaalid ja konsonandid olid palatalsatsioonipaarides ja i-tüvelistes nimisõnades lühemad kui emakeelsetel kõnelejatel.
In-situ measurements of wall moisture in a historic building in response to the installation of an impermeable floor
Authors: Briggs Kevin , Ball Richard , McCaig Iain 
Published: July 2022

When impermeable ground bearing slabs are installed in old buildings without a damp-proof course, it is a common belief of conservation practitioners that ground moisture will be ‘driven’ up adjacent walls by capillary action. However, there is limited evidence to test this hypothesis.
An experiment was used to determine if the installation of a vapour-proof barrier above a flagstone floor in a historic building would increase moisture content levels in an adjacent stone rubble wall. This was achieved by undertaking measurements of wall, soil and atmospheric moisture content over a three-year period. Measurements taken using timber dowels showed that the moisture content within the wall did not vary in response to wall evaporation rates and did not increase following the installation of a vapour-proof barrier above the floor. This indicates that the moisture levels in the rubble wall were not influenced by changes in the vapour-permeability of the floor.
AUTOMEDICAÇÃO E SEUS RISCOS À SAÚDE DURANTE A PANDEMIA DA COVID-19: REVISÃO INTEGRATIVA
Authors: Alencar Gustavo de Oliveira , Alencar Jean Pierre de Oliveira , Paiva Larissa Varela de , Mori Edna , Mendes Rafael de Carvalho 
Published: July 2022

Automedicação é o ato de ingerir medicamentos, sem qualquer orientação do profissional adequado. Durante a pandemia da COVID-19 ocorreram muitos casos relacionados à hidroxicloroquina, cloroquina, azitromicina, ivermectina, nitazoxanida, além de polivitamínicos contendo zinco, vitamina C e D. O objetivo deste trabalho foi realizar uma revisão sobre a automedicação e os riscos à saúde durante a pandemia da COVID-19. Tratou-se de uma revisão integrativa de caráter qualitativo, pesquisando trabalhos científicos relacionados à automedicação e a COVID. As bases de dados utilizadas foram: LILACS (Literatura Científica e Técnica da América Latina e Caribe), MEDLINE (Medical Literature Analysis and Retrieval System Online) e SciELO (Scientific Electronic Library Online). Os descritores aplicados foram: Automedicação/COVID e Self-medication/COVID. Dos 558 artigos, publicados entre 2019 a 2021, disponíveis na íntegra gratuitamente, no idioma português e inglês, 9 foram selecionados, conforme o protocolo de pesquisa. Os estudos abordaram a prática da automedicação no período da pandemia para medidas preventivas. Dentre as classes farmacêuticas abordadas no estudo foram: antimaláricos, antirretrovirais, antibacterianos, vitaminas, analgésicos e antipiréticos. Campanhas educativas são necessárias, uma vez que o uso inadequado pode gerar resistência bacteriana, intoxicações por superdosagem, efeitos colaterais e até mesmo piora do quadro de alguma doença já existente.
Global Warming and Border Carbon Adjustments
Authors: Hong Sungwan , Sim Seung-Gyu , Obashi Ayako , Tsuruta Yoshitaka 
Published: July 2022

Abstract
This study examines the welfare implications of allowing border carbon adjustments (BCAs) in a globalized economy characterized by international trade and cross-border pollution (CBP). The model predicts that adopting BCAs is a weakly dominant strategy and global welfare is maximized when at least one country adopts BCAs in the presence of CBP, such as global warming. This is because adopting BCAs induces other countries to raise their domestic emission tax rates without concerns such as the excessive shrinkage of domestic production and aggravation of CBP.
Automatic landmark identification for surgical 3d-navigation – A proposed method for marker-free dental surgical navigation systems
Authors: Bischofberger Micha , Böhringer Stephan , Schkommodau Erik 
Published: July 2022

Abstract
This paper proposes a conceptual method to calculate the pose of a stereo-vision camera relative to an artificial mandible without additional markers. The general method for marker-free navigation has four steps: 1) parallel image acquisition by a stereo-vision camera, 2) automatic identification of 2d point pairs (landmark pairs) in a left and a right image, 3) calculation of related 3d points in the joint camera coordinate system and 4) matching of 3d points generated to a preoperative 3d model (i.e., CT data based). To identify and compare landmarks in the acquired stereo images, well-known algorithms for landmark detection, description and matching were compared within the developed approach. Finally, the BRISK algorithm (Leutenegger S, Chli M, Siegwart RY. BRISK: Binary Robust invariant scalable keypoints. Proceedings of the IEEE International Conference on Computer Vision; 2011: 2548–2555) was used. The proposed method was implemented in MATLAB® and validated in vitro with one artificial mandible. The accuracy evaluation of the camera positions calculated resulted in an average deviation error of 1.45 mm ± 0.76 mm to the real camera displacement. This value was calculated using only stereo images with over 100 reconstructed landmark pairs each. This provides the basis for marker-free navigation.
Serum free light chain analysis: persisting limitations with new kids on the block
Authors: Van Hoovels Lieve , Vercammen Martine , Nevejan Louis , Cornette Margot , Briers Pieter-Jan , Deeren Dries , Van Droogenbroeck Jan , Fostier Karel , De Smet Dieter 
Published: July 2022

Abstract

Objectives
Serum free light chain (sFLC) measurements have inherent analytical limitations impacting sFLC clinical interpretation. We evaluated analytical and diagnostic performance of three polyclonal sFLC assays on four analytical platforms.

Methods
sFLC concentration was measured using Diazyme FLC assays (Diazyme) on cobas c501/c503 analyzer (Roche); Freelite assays (The Binding Site) on Optilite analyzer (The Binding Site) and cobas c501 analyzer and Sebia FLC ELISA assays (Sebia) on AP22 ELITE analyzer (DAS). Imprecision, linearity, method comparison vs. Freelite/Optilite, antigen excess detection and reference value verification were assessed. Diagnostic performance was compared on 120 serum samples and on follow-up samples of five patients with κ and λ monoclonal gammopathy.

Results
Method comparison showed excellent correlation with Freelite/Optilite method for all assays. A large proportional negative bias was shown for both Sebia κ and λ ELISA and a significant positive proportional bias for λ in the low (<10 mg/L) Freelite/cobas c501 method. Clinically relevant underestimation of κ sFLC levels due to antigen excess was shown for 7% of each Diazyme/cobas application and for 11 and 32.1% of λ sFLC assay of respectively Diazyme/cobas and Sebia/AP22. sFLC reference values revealed application specific. Cohen’s κ values were (very) good for κ sFLC but only moderate to good for λ sFLC. In 4/10 follow-up patients, significant differences in clinical interpretation between sFLC assays were noticed.

Conclusions
Important analytical limitations remain for all sFLC applications. Differences in reference values and diagnostic performance hamper interchangeability of sFLC assays. Assay specific sFLC decision guidelines are warranted.
A Late Etruscan Burial at Podere Cannicci (Civitella Paganico – Grosseto)
Authors: Sebastiani Alessandro 
Published: July 2022

Abstract
In March 2021, following some agricultural activities on a slight relief in the area of Civitella Paganico, the remains of a second century B.C.E. burial were excavated by the research team of the IMPERO Project (Interconnected Mobility of People and Economies along the River Ombrone). This paper details the results of the rescue excavations and presents the preliminary data on the nearby settlement of Podere Cannicci to contextualize its necropolis. Finally, the paper addresses some initial thoughts on the broader importance of the discovery in terms of settlement networks and spheres of cultural influences in this liminal area.
Influence of heterogeneity on fluid property variations in carbonate reservoirs with multistage hydrocarbon accumulation: A case study of the Khasib formation, Cretaceous, AB oilfield, southern Iraq
Authors: Wang Qiang , Wen Tao , Li Hongxi , Zeng Xinyao , Wang Xingzhi , Xin Jun , Sun Li , Wang Chunpu , Zhou Yuezong 
Published: July 2022

Abstract
The fluid distribution is considerably influenced by the reservoir homogeneity. This study analyzes carbonate reservoir heterogeneity and hydrocarbon accumulation processes in the Khasib reservoirs in AB oilfield in the Middle East to discuss factors controlling the distribution of fluid and the effects of this distribution on the productivity of single wells. The Khasib reservoirs in the AB oilfield show uniform vertical distributions, with a large difference in the longitudinal pore throats, and the upper part of the reservoir is better than the lower one. Because the hydrocarbon accumulation is multiphasic, the heterogeneity of crude oil is based on whether traps are formed in the early or late stage of source rock formation during accumulation. Moreover, the longitudinal physical differences in crude oil properties are mainly attributed to the longitudinal differences in the pore throats. The difference in the pore throat and physical properties of crude oil affords differences in the productivity of horizontal wells in different subzones.
Early origins of respiratory disease
Authors: Greenough Anne , Milner Anthony David 
Published: July 2022

Abstract
Chronic respiratory morbidity is unfortunately common in childhood, particularly in those born very prematurely or with congenital anomalies affecting pulmonary development and those with sickle cell disease. Our research group, therefore, has focused on the early origins of chronic respiratory disease. This has included assessing antenatal diagnostic techniques and potentially therapeutic interventions in infants with congenital diaphragmatic hernia. Undertaking physiological studies, we have increased the understanding of the premature baby’s response to resuscitation and evaluated interventions in the delivery suite. Mechanical ventilation modes have been optimised and randomised controlled trials (RCTs) with short- and long-term outcomes undertaken. Our studies highlighted respiratory syncytial virus lower respiratory tract infections (LRTIs) and other respiratory viral LRTIs had an adverse impact on respiratory outcomes of prematurely born infants, who we demonstrated have a functional and genetic predisposition to respiratory viral LRTIs. We have described the long-term respiratory outcomes for children with sickle cell disease and importantly identified influencing factors. In conclusion, it is essential to undertake long term follow up of infants at high risk of chronic respiratory morbidity if effective preventative strategies are to be developed.
Diagnostic value of long noncoding RNA LINC01060 in gastric cancer
Authors: Huang Junhui , Wu Junrong , Hu Zuojian , Mo Cuiju , Chen Huaping , Lu Liuyi , Chen Mingxing , Huang Xiamei , Qin Xue 
Published: July 2022

Abstract

Objectives
Gastric cancer (GC) is a common gastrointestinal tumor that threatens human health. The sensitivity and specificity of traditional tumor markers do not meet the requirements for detection of GC. Long noncoding RNAs (lncRNAs) are crucial for the development of tumors. Hence, in this study, LINC01060 will be evaluated for its diagnostic value in GC.

Methods
Quantitative real-time PCR (qRT-PCR) was used to determine the relative expression levels of LINC01060 in GC tissues and blood samples. In addition, the relationship between the expression levels of LINC01060 and the clinicopathological features of the patients was analyzed. Receiver operating characteristic (ROC) curves were utilized to assess the diagnostic efficacy of LINC01060, carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) in GC.

Results
The expression of LINC01060 was found to have decreased in GC tissues (p<0.01). Notably, compared with peptic ulcers and healthy controls, the expression levels of LINC01060 in the whole blood of GC patients was found to have decreased. Furthermore, LINC01060 exhibited higher sensitivity and specificity than CEA and CA19-9 in differentiating GC from healthy controls (AUC=0.872, sensitivity=96.0%, specificity=76.7%). With regard to traditional biomarkers, the AUC of CEA and CA19-9 were 0.715 (sensitivity=86.0%, specificity=50.0%) and 0.634 (sensitivity=63.0%, specificity=76.0%), respectively. Remarkably, the other gastrointestinal tumors did not show any statistically significant differences. The presence of LINC01060 was not found to be significantly associated with characteristic clinicopathological factors.

Conclusions
LINC01060 might be useful as a biomarker for diagnosing GC and distinguishing GC patients from healthy controls.
Crystal structure of catena-poly[(μ
2-4,4′-bipyridine-κ2
N:N′)-bis(4-bromobenzoato-κ1
O)zinc(II)], C24H16Br2N2O4Zn
Authors: Yang Xiuyan , Luo Yingchun 
Published: July 2022

Abstract
C24H16Br2N2O4Zn, monoclinic, I2/a (no. 15), a = 7.4191(9) Å, b = 24.458(3) Å, c = 25.810(3) Å, β = 92.426(3)°, V = 4679.2(10) Å3, Z = 8, R

gt
(F) = 0.0514, wR

ref
(F
2) = 0.1710, T = 296(2) K.
On theoretical analysis of nonlinear fractional order partial Benney equations under nonsingular kernel
Authors: Shah Kamal , Seadawy Aly R. , Mahmoud Anhar B. 
Published: July 2022

Abstract
In the present article, the first step is devoted to develop some results about existence and uniqueness of solution to a general problem of fractional order partial differential equations (FPDEs) via classical fixed point theory. In the second step, a novel technique is used to handle the semi-analytical approximate solution for the considered general problem. Then, we extend the said result to fractional order partial Benney equations (FOPBEs) of the second and third order, which are special cases of the general problem we considered. We study the proposed problem under the Caputo-Febrizo fractional derivative (CFFD). With the help of the proposed method, we derive a series type approximate (semi-analytical) solution. Some numerical interpretations and visualizations are also given.