bluuebunny/crossref_metadata_2025_split huggingface dataset:
The future Alert of Inter-arm blood pressure difference Among young healthy population: A Cross-sectional Study
Authors: Essa Rawand , Ahmed Sirwan 
Published: February 2022

Abstract
Background: More than 100 years ago, the difference in blood pressure (BP) between arms was first reported. Recent studies have shown that different blood pressure between the right and left arm leads to cardiovascular events. The aim of the present study was to establish of an inter-arm blood pressure difference (IAD) and the dominant hands effect on the elevation blood pressure among young healthy population.Methods: This was a cross-sectional study using quantitative method for both data collection and data analysis and carried out at the Sulaimanyah governorate Kurdistan Region, Iraq among a young, healthy population. Data were expressed as percentages of individuals with systolic IAD and diastolic IAD and mean (± standard deviation) for the inter-arm difference in systolic blood pressure and diastolic blood pressure (DBP). To evaluate the association between IAD and age, BMI, heart rate, systolic BP, and diastolic BP Pearson’s correlation test was used. The Chi-square test was used to compare the prevalence inter-arm difference category between groups.Results: Three thousand and thirty volunteers participated in our study. The sIABP was equal in 163 of 3030 persons (5.37%), dIABP was equal in 222 out of 3030 persons (7.32%), from a total of 792/3030 persons (26.1 %) sIAD > 10 mmHg, and dIAD > or =10 mmHg was found in 927 out of 3030 persons (33.5%) in the right arm, and 32.4% in the left arm. In 2692 of 3030 volunteers BP, initially recorded in the dominant hand (right arm), showing sIAD> or = 10 mmHg was found in 943 (37.1%) volunteers, and when the first measurement was done in 338 left-handed volunteers it showed sIAD> or = 10 mmHg in 112 of 338 (34.1%), P < 0.001; 95% confidence interval for systolic right hand were (115.73: 116.73), and for systolic left hand 95% confidence interval were (113.17:114.15).Conclusion: significant inter-arm systolic and diastolic BP differences above (10 mm Hg) is common in the young, healthy population. Hand dominance is a significant consideration while measuring blood pressure. It is mandatory to measure blood pressure in both arms in a sitting position with a stable condition.
Heritability and Multipoint Linkage Analysis Suggest the Contribution of Angiopoietin-TEK Pathway to the Variation of Serum Creatinine and Glomerular Filtration Rate
Authors: Zadjali Fahad , Al-Wahaibi Mazin , Hassan Mohammed O. , Bayoumi Riad A. , Zadjali Razan , Albarwani Sulayma , Riyami Nafila B Al , Al-Yahyaee Saeed 
Published: February 2022

Abstract
Background: Serum creatinine and estimated glomerular filtration rate (eGFR) are keys to assess kidney function and suggest a propensity for development of renal failure. Genetic factors are reported in different ethnic groups to play a role in the variability of these phenotypes. In this study, we investigate the heritability and quantitative trait loci (QTL) of serum creatinine and eGFR. Multiple phenotypes were collected from a multi-generation pedigree of 281 subjects form Oman family study. Genotype analysis was used to calculate heritability and linkage was performed using variance components decomposition-based methods implemented in SOLAR. Results: The multivariate-adjusted heritability estimates for serum creatinine and eGFR were 0.70 and 0.63 (p-value 2.5x10-11 and 1.8x10-11), respectively. Genome-wide linkage analysis showed significant loci with LOD score ≥2 at chromosomes 9p21.1, 9p21.3, 15p26.3 and 16.p13.3. Functional annotation identified angiopoietin 1-TEK pathway as a candidate pathway where TEK is associated with chronic kidney disease and expressed in renal glomeruli. Conclusion: This study showed high heritability of serum creatinine and eGFR with significant QTLs in chromosomes 9, 15 and 16. Further research is needed to study the genetic association studies of angiopoietin 1-TEK axis with glomerulopathies like type 2 diabetes and to predict and diagnose progression of renal diseases.
Real-Time Indirect Continuous Arterial Blood Pressure Measurements From ECG And PPG Waveforms Using Deep Learning
Authors: Seo Hyo Chang , Kim Hyun Bin , Nam Gi-Byoung , Cho Min Soo , Joo Segyeong 
Published: February 2022

Abstract
It is a well-known fact that it is important to accurately measure blood pressure. In particular, it is an even more important vital sign for the elderly and patients in hospitals. Non-invasive techniques for blood pressure measurement only afford discrete results. Although invasive measurement techniques overcome this issue and provide continuous results, they involve the risk of bleeding or infection and also cause discomfort to the patient. To address this issue, in this study, we developed a deep learning model that can estimate the arterial blood pressure (ABP) in real time using the waveform signals from electrocardiograms (ECGs) and photoplethysmograms (PPGs), without handcraft setting. Data pertaining to patients with various disorders admitted in the intensive care unit (necg, ppg= 1,126,870) were used. The performance of the model was evaluated (RSBP= 0.96, RMAP=0.92, and RDBP = 0.90) and verified to meet international standards. Even if the actual value changes dramatically, the estimated value shows a graph that follows the trend. Additionally, the ABP of patients with atrial fibrillation could be measured continuously and in real time.
De novo transcriptome assembly of the cotyledon of Camellia oleifera for discovery of genes regulating seed germination
Authors: Long Wei , Yao Xiaohua , Wang Kailiang , Sheng Yu , Lv Leyan 
Published: February 2022

Abstract
Background: Camellia oleifera (C. oleifera) is one of the most important wood oil species in the world. It was propagated by nurse seedling graft in C.oleifera. Since morphology of rootstock has a significant impact on grafting efficiency and seedling quality, it is necessary to have a clear understanding molecular mechanism of morphogenesis for cultivating high quality and controllable rootstocks,. However, the genomic resource for this species is relatively limited, thus hampering our understanding of the molecular mechanisms of seed germination in C.oleifera. Results: In this paper, we measured the gene expression in the C.oleifera cotyledon in different development stages and the global gene expression profiles through transcriptome sequencing. Approximately 45.4 gigabases (GB) paired-end clean reads were obtained and assembled into 113,582 unigenes with an average length of 396 bp. 61.5% (68,217) of the unigenes were annotated in six public protein databases. We identified 11,391 differentially expressed genes (DEGs) between the different stages in the germination process. Enrichment analysis revealed that the DEGs were mainly involved in hormone signal transduction and starch sucrose metabolism pathways. The gravitropism regulator UNE10, the meristem regulator STM, KNAT1, PLT2, and root-specific transcription factor WOX11 has higher expression level in the CAM2 stage (seed soaking), indicating the cotyledon-regulated program upon seed germination has been initiated to establish in early imbibition. Our data showed that multiple hormone-related genes in cotyledons were differentially reprogrammed during C. oleifera seed germination.Conclusion: The cotyledons play key roles, both as the main nutrient provider and as one of the main instructors for seed germination and seedling growth. Together, our study will remarkably enrich the genomic resources of Camellia and contribute to our understanding of the molecular mechanisms of the development in the seed germination and seedling growth of C.oleifera. It is helpful to culture in standardizing and superior quality rootstock for C. oleifera breeding.
A High-quality Genome Assembly and Annotation of Quercus Acutissima Carruth
Authors: Liu Dan , Zhou Chengcheng , Tong Boqiang , Xie Xiaoman , Yang Haiping , Han Biao , Lu Yizeng , Li Wei , Li Wenqing 
Published: February 2022

Abstract
Background: Quercus acutissima is an economic and ecological tree species often used for afforestation of arid and semi-arid lands and is considered an excellent tree species for soil and water conservation. However, the Q. acutissima genome sequencing and assembly have not been reported, which limiting molecular biology studies and breeding progress in Q. acutissima.Results: We generated a 956.9 Mb genome with a contig N50 of 1,203,427 bp and 53,304 annotated genes. Meanwhile, the assembled contigs (1,507) assigned to a total of 12 chromosomes. The BUSCO database evaluation accounted for 98.0% of the genome size and the genome heterozygous rate was 0.25%. Repetitive sequences constituted 57.13% of the assembled genome, the most abundant one was long terminal repeats (7.18% of the genome). Phylogenetic analysis showed that Q. acutissima and Fagus sylvatica were differentiated around 54.2 million years ago (Mya), and Q. sober was differentiated around 4.4 Mya. Conclusion: We sequenced, assembled and annotated the first high-quality genome of Q. acutissima. This work not only promises to accelerate molecular biology studies and breeding progress in Q. acutissima, Q. acutissima is one of the important species in Quercus, but also promote evolutionary studies at the genome level in the genus Quercus due to its importance as one of the important species in Quercus.
Preliminary Exploration Of Theory And Practice Training Of 5G Ultrasonic Remote Consultation In The Grassroot Hospitals
Authors: Wang Ceng , Zheng YI , Xiong Cui , Wang Jing 
Published: February 2022

Abstract
Background: Many people in rural areas have not had an ultrasound examination for a long time due to lack of medical resources. So we go through training doctors in grassroot hospitals to explore the clinical and practical value of remote ultrasound.Methods: The Superior hospital will conduct ultrasonic theory and practice training for the grassroot hospitals. After passing the training, remote ultrasonic diagnosis will be carried out at the grassroot level, then feedback and case data will be collected.Results: Through training, the ultrasonic diagnosis level of the grassroot doctors has been significantly improved. At present, a total of 148 cases of consultation have been completed through remote ultrasound "visual apparatus", mainly abdominal ultrasound, and the common cases are fatty liver, hepatic cyst, gallbladder stone, kidney stone and so on . Conclusion: As a "visual apparatus", remote ultrasound can be routinely carried out in the grassroot hospitals, which can promote mutual benefit between doctors and patients and comprehensively promote the construction of healthy villages.
A Nomogram Model Developed and Validated for The Evaluation of Lymph Node Metastasis in Patients with Rectal Cancer
Authors: Su Yexin , Zhao Hongyue , Zhang Linhan , Jiao Yuying , Xu Peng , Lyu Zhehao , Liu Pengfei , Fu Peng 
Published: February 2022

Abstract
Purpose: The aim of this study was to develop and validate a nomogram model to evaluate lymph node metastasis (LNM) in patients with rectal cancer (RC).Methods: A total of 162 patients with RC between 2019 and 2021 were included in the study. Patients were allocated to a training set and a validation set at a ratio of 7:3. The lymph node (LN) status was evaluated retrospectively from magnetic resonance imaging (MRI) images by two radiologists. Based on 103 radiomic features extracted from T2 weighted images (T2WI), the least absolute shrinkage and selection operator (LASSO) was used to screen and calculate the radiomic feature score (Radscore). The model was constructed using the logistics regression algorithm. The DeLong test and decision curve analysis (DCA) were used to compare the prediction performance and clinical utility of the MRI reported model, the Radscore model, and the complex model constructed by combining the MRI reported and Radscore. The nomogram model was constructed to visualize the prediction results of the best model. Model performance was evaluated in the training and validation groups, and the calibration curve and Hosmer-Lemeshow goodness of fit test were used to evaluate the calibration.Result: This study included 162 patients with RC, including 54 patients with LNM and 108 patients without LNM. All three models constructed by the logistics regression algorithm were good at identifying LNM. The DeLong test and the DCA results showed that the complex model outperformed the MRI-based model and the Radscore model in relation to their predictive performance and clinical utility. The nomogram of the complex model had an area under the curve (AUC) of 0.902 (95% confidence interval (CI): 0.848−0.957) in the training group and an AUC of 0.891 (95% CI: 0.799−0.983) in the validation group. Meanwhile, the calibration curve and the Hosmer-Lemeshow goodness-of-fit test showed good calibration.Conclusion: The nomogram model constructed based on T2WI radiomics and MRI reported had good diagnostic efficacies for LNM in patients with RC, and provided a new auxiliary method for accurate and individualized clinical management.
The Frequency of Os Acromiale in 751 Shoulder MRI of Saudi Population
Authors: Aldebeyan Wassim , mogbil Ismail , AlShayhan Fahad , Alzahim Mohammed , Alshunaifi Abdullah , Alhowaimel Abdulaziz , Al-Nakshabandi Nizar 
Published: February 2022

Abstract

Objectives: Os acromiale (OA) is an uncommon pathology, with a variable prevalence rate among different populations. reporting the frequency of OA utilizing shoulder MRI of patients with shoulder pathology in our institute. Methods: Retrospective chart review to conduct our research. After obtaining our IRB approval we gathered all shoulder and upper arm MRIs from the radiology department and available in PACS. Results: The prevalence of OA was 3.32%, and the mean age of the affected patients was 50.87 years (25–81). In males there were seven OA conditions
Cyprideis Torosa (Ostracoda, Crustacea): Occurrence Patterns, Carapace Type And Its Relationship To Physicochemical Variables In Kocaçay Delta (Turkey)
Authors: Külköylüoğlu Okan , Yavuzatmaca Mehmet , Akay Enis , Dalkıran Nurhayat 
Published: February 2022

Abstract
To understand spatial and temporal continuing occurrence patterns and relationships of Cyprideis torosa to several physicochemical variables, monthly samples from seven stations were collected from Kocaçay Delta (Bursa, Turkey) between 2018 and 2019. Four (C. torosa, Koencypris ornata, Candona lindneri, Candona meerfeldiana) of 14 ostracod taxa are new records for the ostracod fauna of Bursa province where total numbers of recent species increased to 33. Cyprideis torosa was the only dominant species found in almost all samples throughout the sampling. Based on carapace type of the species, three groups can be divided as i) Type-1 (smooth carapaces), ii) Type-2 (noded carapaces), and iii) Type-3 (both carapaces noded on one valve and smooth on the other). Different occurrence patterns of the species with overlapping ecological ranges were observed among the stations. Except for station 1, all the types were encountered from other stations. Beginning from December 2018, nearly all populations had mostly Type-1 individuals until the end of March. During April-May, individuals with Types-2 and 3 appear to increase until the end of October while individuals in Type-2 group were solely found from three stations (2, 3 and 5) in March and May 2018. There was a significant difference in salinity, magnesium, and calcium values among the stations (P < 0.05) but only total nitrogen, temperature and calcium showed a medium correlation to carapace type. In all cases, populations with noded individuals were found in narrower ecological ranges for those variables than other populations with smooth individuals.
Performance, eating behavior and carcass characteristics of feedlot lambs fed diets without forage containing sodium bicarbonate
Authors: VICENTE ANA CAROLINA SILVA , Carlis Matheus Sousa de Paula , Santos Isabela Jorge dos , Silva Adrielly Lais Alves da , Júnior Paulo César Gonzales Dias , de Assis Rhaissa Garcia , Sturion Thamires Ubices , Biava Janaina , Pires Alexandre Vaz , Ferreira Evandro Maia 
Published: February 2022

Abstract
The study was carried out to evaluate the effect of inclusion of sodium bicarbonate (SB) in diets based on ground corn without forage for feedlot lambs. Twenty-eight ram lambs (Dorper x Santa Inês), with initial body weight of 30.2 ± 4.5 kg and 94 ± 8.4 days old were used. They were assigned to a randomized complete block design. Experimental diets were positive control, diet without forage containing soybeans hulls replacing ground flint corn in 400g/kg of dry matter (DM) (CONT); and three diets without forage based on ground flint corn containing 10 (10SB), 20 (20SB) or 30 (30SB) g/kg DM of SB. The inclusion of SB quadratically increased final body weight (P = 0.02), dry matter intake (DMI; P &lt; 0.01), average daily gain (P &lt; 0.01), feed eficiency (P &lt; 0.01), slaughter body weight (P = 0.02), hot and cold carcass weight (P = 0.02), loin muscle area (P = 0.05), leg compacity index (P = 0.05) and the weights of: neck (P = 0.01), shoulder (P = 0.02), rib (P = 0.03) and leg (P = 0.01), with higher values for the 20SB. Feeding SB linearly decreased the area (P &lt; 0.01) and height (P = 0.01) of the papillae. The use of SB improved the performance and increased hot and cold carcass weights, with better results observed in the 20 g/kg level (20SB), showing to be efficient in diets without forage for feedlot lambs.
Bacterial Dynamics During the Degradation of Starch-based Bioplastic and Oxo- degradable Plastic in Compost Soil
Authors: Wicaksono Joshua Abednego , Purwadaria Tresnawati , Yulandi Adi , Tan Watumesa Agustina 
Published: February 2022

Abstract
Background: Plastic waste accumulation is one of the main ecological concerns in the past decades. A new generation of plastics that are easier to degrade in the environment compared to conventional plastics, such as starch-based bioplastic and oxo-degradable plastic, is perceived as a solution to this issue. However, the main degraders of such materials and their fate in the environment are unclear. In this study, we monitored the dynamics of bacterial community in soil upon introduction of commercial starch-based bioplastic and oxo-degradable plastic. The plastics were buried separately in compost soil and incubated for 30, 60, 90, and 120 days. Following incubation, soil pH and temperature as well as the weight of remaining plastics were measured. Bacterial diversity in soil surrounding the plastics was analyzed using Illumina high-throughput sequencing of the V3-V4 region of 16SrDNA. Results: Starch-based bioplastic degraded quicker than oxo-degradable plastic in soil. The bacterial composition in soil fluctuated over time with or without the introduction of either type of plastic. While the major bacterial phyla remained similar for all treatment in this study, different types of plastics led to different soil bacterial community structure. None of these bacteria were abundant continuously, but rather they emerged at specific time points. The introduction of plastics into soil increased not only the population of bacteria with the ability to directly utilize plastic component for their growth, but also the abundance of those that may interact with direct degraders. Interestingly, there were no specific genera that were prevalent throughout the burial period. Conclusions: The degradation of both starch-based bioplastic and oxo-degradable plastic involves a complex set of bacteria that continues to shift over time.
Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement
Authors: Yu Hang , Wei Xiaoxi , Wu Zhina , Jiang Huan , Qi Huichuan , Zhang Yi , Hu Min 
Published: February 2022

Abstract
In orthodontic treatment, alveolar bone remodeling is the basis of tooth movement, therefore, exploring the impact of mechanical force on bone remodeling and related signaling pathways are of great importance. EphrinB2-EphB4 bidirectional signaling plays an important role in communication between osteoblasts and osteoclasts. However, whether ephrinB2-EphB4 signaling is involved in the osteogenic differentiation promoted by tensile force (TF) is unclear. Therefore, in this study, we first explored the effect of TF on osteogenic differentiation in cells, and found that TF significantly increased the expression levels of RUNX2, OPN and EphB4 in MC3T3-E1 cells, but when ephrinB2-EphB4 signaling was blocked by NVP-BHG712, TF-induced promotion of osteogenic differentiation was inhibited. Then we established a rat orthodontic tooth movement (OTM) model, through Micro CT and H&E staining, we found that TF improved alveolar bone formation through ephrinB2-EphB4 signaling.MAPK members are involved in osteogenic differentiation, and we found that TF promoted the expression of p-ERK1/2, p-P38 and p-JNK1/2/3. When EphB4 receptor was blocked, the expression of p-ERK1/2 promoted by TF was decreased, and it was the downstream pathway of ephrinB2-EphB4 signaling that mediates TF-induced promotion of osteogenic differentiation in MC3T3-E1 cells.
COVID-19 Contact Tracing in Belgium: Main Indicators and Performance, January - September 2021.
Authors: Proesmans Kristiaan , Hancart Sharon , Braeye Toon , Klamer Sofieke , Robesyn Emmanuel , Djiena Achille , De Leeuw Frances , Mahieu Romain , Dreuw Alex , Hammami Naima , Wildemeersch Dirk , Cornelissen Laura , Cauteren Dieter Van 
Published: February 2022

Abstract
Background: Contact tracing is one of the main public health tools in the control of coronavirus disease 2019 (COVID-19). A centralized contact tracing system was developed in Belgium in 2020. We aim to evaluate the performance and describe the results, between January 01, 2021, and September 30, 2021. The characteristics of COVID-19 cases and the impact of COVID-19 vaccination on testing and tracing are also described.Methods: We combined laboratory diagnostic test data (molecular and antigen test), vaccination data, and contact tracing data. A descriptive analysis was done to evaluate the performance of contact tracing and describe insights into the epidemiology of COVID-19 by contact tracing. Results: Between January and September 2021, 555.181 COVID-19 cases were reported to the central contact center and 91% were contacted. The average delay between symptom onset and contact tracing initiation was around 5 days, of which 4 days corresponded to pre-testing delay. High-Risk Contacts (HRC) were reported by 49% of the contacted index cases. The mean number of reported HRC was 2.7. In total, 666.869 HRC were reported of which 91% were successfully contacted and 89% of these were tested at least once following the interview. The estimated average secondary attack rate (SAR) among the contacts of the COVID-19 cases who reported at least one contact, was 27% and was significantly higher among household HRC. The proportion of COVID-19 cases who were previously identified as HRC within the central system was 24%.Conclusions:The contact-tracing system contacted more than 90% of the reported COVID-19 cases and their HRC. This proportion remained stable between January 1 2021 and September 30 2021 despite an increase in cases in March-April 2021. We report high SAR, indicating that through contact tracing a large number of infections were prospectively detected. The system can be further improved by (1) reducing the delay between onset of illness and medical consultation (2) having more exhaustive reporting of HRC by the COVID-19 case.