bluuebunny/crossref_metadata_2025_split huggingface dataset:
The combination of fruquintinib, raltitrexed and S-1 as a third-line treatment for metastatic colorectal cancer: A case report
Authors: Wan Yuming , Luo Deyun 
Published: July 2022

Abstract
Effective late-line therapies for metastatic colorectal cancer (mCRC) are urgently needed. This case report presents a patient with mCRC who received third-line treatment with fruquintinib, raltitrexed and S-1. A 54-year-old male presenting with hematochezia was admitted to our hospital in June 2017 and underwent surgery for a tumor between the rectum and sigmoid colon. Postoperative pathology identified adenocarcinoma (wild-type RAS/RAF, no PIK3CA mutation), and the patient was diagnosed with mCRC (pathological stage, pT3pN1apM0). The mFOLFOX6 regimen was administered. The patient was subsequently diagnosed with Hodgkin lymphoma in May 2018 and treated with the ABVD regimen after multidisciplinary discussions. Liver metastases (intestinal-type adenocarcinoma) were detected in November 2018, and second-line therapy with the FOLFIRI regimen was initiated in January 2019. Lung metastases were identified in September 2019, so the patient was treated with the combination of raltitrexed, S-1 and fruquintinib. A partial response was detected in November 2019, and the patient underwent resection of the hepatic lesion on November 5, 2020. CT images in November 2021 revealed stable disease, thus raltitrexed was discontinued and S-1 and fruquintinib was maintained. The patient remains alive at the time of writing. The case was characterized by the simultaneous existence of mCRC and Hodgkin lymphoma, which required management by a multidisciplinary team. Third-line therapy with fruquintinib, raltitrexed and S-1 achieved a partial response that permitted surgical resection as well as a relatively long progression-free survival. This regimen may have potential as a late-line therapy for mCRC.
Identification of a upstream transcription complex regulating miR-9 expression during neurogenesis
Authors: Kuriakose Diji , Iraqi Fuad , Morahan Grant , Xiao Zhicheng 
Published: July 2022

Abstract
MicroRNAs (miRs) are critical for many cellular processes, including neurogenesis. Little is known about the components of upstream regulation of miR. We studied regulation of miR-9, which is highly expressed in the brain. Using Collaborative Cross mice, significant quantitative trait loci (QTL) were mapped to regions containing 131 candidate genes. These were characterized by RNAi interference, qPCR and neuronal differentiation assays to derive a short list of candidates. Three of these encoded proteins (Panx2, Polr1c and Mgea5) were shown by ChIP-seq and Co-IP to associate mutually with each other and bind to the miR-9 locus. We named this DNA-protein interaction complex that acts upstream of miR-9, which serves as the site for regulating miRs' expression, “miRSome”. A 3C/ChIP-loop assay confirmed the chromatin organisation of the miRSome on the miR-9 locus. These results describe the components of upstream regulatory mechanism of miR-9 expression during neurogenesis.
Extracting potential new targets for treatment of Adenoid Cystic Carcinoma using bioinformatic methods
Authors: Pordanjani Tayebeh Forooghi , Dabirmanesh Bahareh , Choopanian Peyman , Mirzaie Mehdi , Mohebbi Saleh , Khajeh Khosro 
Published: July 2022

Abstract
Adenoid cystic carcinoma (ACC) is a slow-growing malignancy that most often occurs in the salivary glands. Although reasonable local control is usually achieved by tumor surgery and subsequent radiation therapy, recurrence at the same or distant site is the cause of treatment failure. Currently, no FDA-approved therapeutic target or diagnostic biomarker has been identified for this cancer. To find the therapeutic and diagnostic targets for ACC, we extracted the gene expression information from two GEO datasets. Different expression genes (DEGs) between ACC and normal samples were extracted and used to explore the biochemical pathways involved in ACC and create a protein-protein interaction (PPI) network.After analyzing the PPI network, 20 hub genes were introduced that have potential as diagnostic and therapeutic target. Among them, PLCG1 and EZH2 were introduced as new biomarkers in ACC that might have a high value in the diagnosis and treatment of ACC. Furthermore, by studying the roles of the hub genes in the enriched biochemical pathways, we found that most likely, IGF-1R/IR and PPARG pathways play a critical role in tumorigenesis and drug resistance in the ACC and have a high potential for selection as a therapeutic target in future studies.
Factors associated with Insomnia among Frontline nurses during COVID-19: A Cross-Sectional Survey
Authors: Nazari Nabi , Sadeghi Masoud , Samusenkov Vadim , Aligholipour Akram 
Published: July 2022

Abstract
Background: Research predominantly suggests that nurses are at high risk of developing psychopathology. The empirical data show that the occurrence rate of problem-related sleep quality among clinical nurses is high. Therefore, this study was conducted to address the lack of information on the relationship between COVID-19 pandemic and Insomnia.Methods: A convenience sample of nurses (n=680) completed an online survey that included the insomnia severity index, the COVID-19-related psychological distress scale, the general health questionnaire, neuroticism, dysfunctional beliefs and attitudes about sleep; and difficulties with emotional difficulties. Results: Results showed that 35.8 % (n=253) of nurses were classified as individuals with moderate to severe clinical Insomnia. The results showed that the psychological distress generated by the COVID-19 predicted the insomnia (β=.47, SE= 0.02, P<.001, t=13.27, 95% CI 0.31-0.46). Also, this association mediated by the psychopathology vulnerabilities, emotion dysregulation, dysfunctional beliefs about sleep, and neuroticism. Moreover, female nurses exhibited higher levels of insomnia (Cohen’s d=.37), dysfunctional beliefs about sleep (Cohen’s d=.21), and neuroticism (Cohen’s d=.29), than males.Conclusion: The findings make a significant contribution to the expanding literature on emotion dysregulation, beliefs, and psychopathology vulnerability on Insomnia. The findings suggest the potential influence of Cognitive behavioural therapy interventions for insomnia (CBT-I) and transdiagnostic integrated therapies that could be incorporated into therapeutic of programs designed to develop as a way of inhibiting or preventing insomnia among clinical nurses.
Antioxidant Properties of Triterpenoids in Fat-Containing Products
Authors: Averyanova Elena , Shkolnikova Marina , Chugunova Olga 
Published: July 2022

Natural antioxidants of plant origin include chemical compounds, extracts, and essential oils isolated from plant raw materials. They are able to inhibit oxidative spoilage in fat-containing foods. The present research featured the antioxidant and in vivo properties of triterpene alcohol botulin, isolated from the bark of Betula pendula Roth., in fat-containing products, e.g., mayonnaise.
Experimental samples of mayonnaise were prepared using the Provencal mayonnaise technology: 6 of 0.2% betulin solution was introduced in vegetable oil during emulsification. The control sample contained no betulin. The experiment relied on standard methods to study the sensory, physicochemical, and microbiological properties of mayonnaise samples. Antioxidant activity was calculated as the inverse of the value of the peroxide number. The antioxidant and hepatoprotective properties underwent hysiological and biochemical in vivo tests on certified white male Wistar rats.
The research resulted in a new formulation of mayonnaise with triterpenoid botulin, which proved to be a good plant antioxidant as it reduced the values of acid and peroxide numbers. In freshly processed samples, the acid number was 0.013 g/dm3 in the experimental sample and 0.033 g/dm3 in the control. After 60 days of storage, it increased by 1.4 and 2.3 times, respectively. The peroxide number was 8–10 times higher in the control sample during the storage period. Betulin inhibited the oxidation of triacylglycerides. The antioxidant activity of the fresh prototype was 5.00 units and 0.50 units after 60 days. In the control, it was 0.50 and 0.06 units, respectively. In the experimental sample, betulin slowed down the growth rate of microorganisms. In the control sample, the yeast content increased 1.6 times, and mold exceeded the permissible value by 20%.
The antioxidant properties of experimental mayonnaise were proven in vivo on a model of acute toxic hepatitis in rats. Based on biochemical studies of animal blood plasma, the use of mayonnaise with betulin improved the antioxidant protection. Betulin had a positive effect on antioxidant enzymes that destroy organic peroxides, e.g., lipid peroxides, which violate the structure of cell membranes.
Bacteriophages of Lactic Acid Bacteria
Authors: Ganina Vera , Mashentseva Natalia , Ionova Inna 
Published: July 2022

Bacteriophages harm food production, disrupt fermenting, spoil dairy products, and cause financial loss. The article describes the biodiversity and properties of bacteriophages capable of lysing lactic acid bacteria used in fermented dairy and meat products.
The research featured bacteriophages obtained from fermented meat and dairy products. The methods included microbiological analyses, sensory evaluation, physico-chemical tests, genetic studies, electron microscopy, optical reassociation, and mathematical data processing.
Violation of the fermentation process always resulted in bacteriophages in the finished products, e.g., fermented dairy products, curd whey, raw smoked and dry-cured sausages, etc. The list of bacteriophages of lactic acid bacteria included 20 bacteriophages of Lactococcus ssp., 11 – of Streptococcus salivarius subsp. thermophilus, and 5 – of Lactobacillus delbrueckii subsp. bulgaricus. The study revealed the diversity of the isolated bacteriophages, their molecular and biological profile, the titer and range of their new hosts, and the differences from standard ones. The bacteriophages proved to be able to infect a wider range of lactic acid bacteria.
The article describes the change in the biodiversity and evolution of bacteriophages depending on their lytic action and virulence. The improved collection of bacteriophages and their detection cultures contribute to an earlier and more effective identification of bacteriophages in fermented products.
Effect of 45 full-scale WWTPs on tropical receiving water bodies in Brazil by partial least squares-discriminant analysis
Authors: Dantas Marina Salim , Magalhães Filho Fernando Jorge Corrêa , Christofaro Cristiano , Oliveira Sílvia Corrêa 
Published: July 2022

Abstract
Treated wastewater may affect water quality and thereby significantly alter physicochemical and biological water quality parameters. The impact of wastewater treatment plants (WWTPs) on receiving water bodies is a multivariate problem. In this study, we investigated the effect of 45 full-scale WWTPs on tropical receiving water bodies in Mato Grosso do Sul State, Brazil. Most of the Pantanal wetland area lies within Mato Grosso do Sul State, thus representing a region of great hydrological relevance. Partial least squares-discriminant analysis (PLS-DA) was employed to discriminate samples collected at four WWTPs monitoring sites: influent, final effluent, upstream, and downstream of the discharges. The model demonstrated excellent accuracy when discriminating the influent from the effluent samples, but poor accuracy when discriminating upstream and downstream samples, indicating the high dilution capacity of the receiving water bodies as a critical factor in the water resources management. The results demonstrate the great potential of the methodology for better water resources management, which can be used in even more complex WWTP databases, allowing the assessment of effluent disposals' impacts in detail. It is recommended to use this methodology in water-limited regions to determine the effect of disposals in areas with different characteristics.
The Protective Effect of Sulforaphane on ER-induced Apoptosis and
Inflammation in Necrotizing Enterocolitis Mice
Authors: Wang Xinhong , Mi Yanhong , Xiong Xiaoyu , Bao Zhongkun 
Published: July 2022

Background:
Necrotizing enterocolitis (NEC) is a neonatal intestinal necrotizing disease caused by various factors in newborns. Sulforaphane (SFN) has a strong anti-inflammatory ability and a certain protective effect on intestinal diseases.

Objective:
NEC is a common developed gastrointestinal exigency in untimely baby. SFN is a naturally originated isothiocyanate that has beneficial effects on the intestinal system.The purpose of this study is to study the protective effect of SFN on endoplasmic reticulum stress (ERS)-related NEC.

Methods:
The newborn mice were randomly divided into control (n=15), NEC (n=20), and NEC+SFN (n=18) groups. Mice in NEC and SFN+NEC groups were injected with 0.1 μl normal saline or 20 mg/kg/d SFN, respectively. After that, the weight and survival in the mice were recorded every day. Then the mice were sacrificed after the 96 h of modeling, ileum tissue and blood samples were collected for qPCR, Western blot, ELISA, HE staining, TUNEL staining, immunohistochemistry assays.

Results:
Results: SFN significantly inhibited the mRNA expression of BIP, CHOP, IL-1β and IL-6, and protein expression of Bax, Caspase-3, Caspase-9 and CHOP, and promoted the expression of Bcl-2 in ER-induced NEC mice intestinal tissues (P<0.01). Meanwhile, SFN could suppress the serum levels of IL-8, IL-10, IL-6, TNF-α, IL-1β, and positive expression of TLR4, and NF-κB (P<0.01), but promote the serum levels of IL-10. HE staining showed that SFN alleviated the NEC intestinal tissue injury, and TUNNEL staining showed that SFN could reduce the rate of NEC apoptotic cells (P<0.01). Moreover, SFN treatment improved the body weight and survival rate in NEC mice.

Conclusion:
SFN could effectively protect ERS-induced inflammation and apoptosis in NEC mice.
MTA1: A Vital Modulator in Prostate Cancer
Authors: Qian Haili , Zhang Yong , Ma Jialu , Li Chunxiao 
Published: July 2022

Abstract:
Prostate cancer (PCa) is the most frequent cancer of the male genitourinary system and the
second most common cancer in men worldwide. PCa has become one of the leading diseases endangering
men's health in Asia in recent years, with a large increase in morbidity and mortality. MTA1
(metastasis-associated antigen-1), a transcriptional coregulator involved in histone deacetylation and
nucleosome remodeling, is a member of the MTA family. MTA1 is involved in cell signaling, chromosomal
remodeling, and transcriptional activities, all of which are important for epithelial cell progression,
invasion, and growth. MTA1 has been demonstrated to play a significant role in the formation,
progression, and metastasis of PCa, and MTA1 expression is specifically linked to PCa bone
metastases. Therefore, MTA1 may be a potential target for PCa prevention and treatment. Here, we
reviewed the structure, function, and expression of MTA1 in PCa as well as drugs that target MTA1 to
highlight a potential new treatment for PCa.
Global Pattern and Trends in Penile Cancer Incidence: Population-Based Study
Authors: Fu Leiwen , Tian Tian , Yao Kai , Chen Xiang-Feng , Luo Ganfeng , Gao Yanxiao , Lin Yi-Fan , Wang Bingyi , Sun Yinghui , Zheng Weiran , Li Peiyang , Zhan Yuewei , Fairley Christopher K , Grulich Andrew , Zou Huachun 
Published: July 2022

Background
Penile cancer is a relatively rare genital malignancy whose incidence and mortality are rising in many countries.

Objective
This study aims to assess the recent incidence and mortality patterns and incidence trends of penile cancer.

Methods
The age-standardized incidence and mortality rates (ASIR and ASMR, respectively) of penile cancer in 2020 were estimated from the Global Cancer Registries (GLOBOCAN) database. Incidence trends of penile cancer from 1973 to 2012 were assessed in 44 populations from 43 countries using the Cancer Incidence in Five Continents plus (CI5plus) and the Nordic Cancer Registries (NORDCAN) databases. Average annual percentage change was calculated to quantify trends in ASIR using joinpoint regression.

Results
Globally, the estimated ASIR and ASMR of penile cancer were 0.80 (per 100,000) and 0.29 (per 100,000) in 2020, equating to 36,068 new cases and 13,211 deaths in 2020, respectively. There was no significant correlation between the ASIR (P=.05) or ASMR (P=.90) and Human Development Index. In addition, 15 countries saw increasing ASIR for penile cancer, 13 of which were from Europe (United Kingdom, Lithuania, Norway, Estonia, Finland, Sweden, Cyprus, Netherlands, Italy, Croatia, Slovakia, Russia, and the Czech), and 2 from Asia (China and Israel).

Conclusions
Although the developing countries still bear the higher incidence and mortality of penile cancer, the incidence is on the rise in most European countries. To mitigate the disease burden resulting from penile cancer, measures to lower the risk for penile cancers, including improving penile hygiene and male human papillomavirus vaccination, may be warranted.
Effects of Novel, Whole-body Movement–Based Interventions on Locomotor Skills in Children With Autism Spectrum Disorder: Randomized Controlled Trial Comparing Face-to-face and Telehealth Modes of Intervention Delivery
Authors: Delepine Emily C , Takillapati Sruthi , Amonkar Nidhi , Su Wan-Chun , Cleffi Corina , Bhat Anjana , Srinivasan Sudha 
Published: July 2022

Background
Autism spectrum disorder (ASD) is a neurodevelopmental disorder limiting a child’s motor performance to a level half their chronological age. Due to COVID-19, there has been growing research on the use of telehealth-based interventions in the care of children with ASD.

Objective
Based on previous research from our lab, this study assessed the effects of 2 novel, whole-body movement interventions (creative movement and general movement) compared to a standard-of-care, seated play intervention using a randomized controlled trial design. Interventions were delivered either face-to-face (F2F) or via telehealth (TH) across all groups.

Methods
In total, 45 children with ASD (aged 5-14 years) participated in a 10-week study. Children were matched on age and level of functioning and then randomly assigned to the Play (creative movement), Move (general movement), or Standard-of-care control (seated play) group (n=15 per group) to receive 8 weeks of intervention F2F or via TH. The interventions were provided twice a week for 8 weeks, with each session lasting for around 1 to 1.5 hours. The locomotor subtest of the Test of Gross Motor Development was administered at pretest and posttest to assess the form and accuracy of locomotor skills (running, galloping, hopping, leaping, horizontal jump, sliding, and skipping).

Results
Improvements were seen in overall standard scores in the Play (pretest: mean 6.53, SE 0.97; posttest: mean 8.60, SE 0.97; P<.05) and Move groups (pretest: mean 6.07, SE 0.96; posttest: mean 8.07, SE 0.92; P<.05) but not in the Create group (pretest: mean 6.13, SE 0.88; posttest: mean 6.80, SE 0.87; P.05). Specifically, 75% of children seen via TH and 57.1% of children seen F2F improved in the Play group, and 89% of children seen via TH and 50% of children seen F2F improved in the Move group.

Conclusions
Our pilot data suggest that TH is a viable option for movement intervention delivery and can be used to promote locomotor skills in children with ASD. Broadly, movement-based interventions must be included in the plan of care for children with ASD, given their significant challenges with movement performance.
Feasibility and Acceptability of Recruitment and Retention in a Remote Trial of Gatekeeper Training for Military Veterans
Authors: Teo Alan Robert , Call Aaron Alexander , Hooker Elizabeth R , Fong Clarissa , Karras Elizabeth , Dobscha Steven K 
Published: July 2022

Background
SAVE (Signs; Ask; Validate; Encourage) is a brief gatekeeper training designed to teach lay individuals how to identify and assist military veterans at risk for suicide. SAVE can be delivered asynchronously using a web-based video format, but no studies of the effectiveness of SAVE exist.

Objective
The aim of this project was to determine the feasibility and acceptability of recruitment and retention in a remote trial of SAVE.

Methods
We conducted a social media campaign using sponsored Facebook posts (ads) to recruit veterans, including those outside the Veterans Affairs network of care, and their loved ones. Participants (N=214) were randomized to SAVE or a sham video training unrelated to suicide prevention and followed for 6 months. We also conducted qualitative interviews with a subgroup (n=15) and used a mixed methods framework to integrate findings.

Results
At baseline, most participants were a family member or friend of a veteran (146/214, 68.2%), and 47.7% (102/214) knew at least one veteran or service member who had died by suicide. Across both study arms, 73.8% (158/214) responded to at least 3 of 6 follow-up surveys and 72.4% (155/214) completed follow-up at 6 months. Themes from interviews indicated the following three barriers to study participation: generic posts, copy (ad text) referring to “research,” and Facebook as a platform. There were 5 facilitators to participation: audience segmentation focused on veterans’ family members and friends, an urgent call to action to help a veteran, prior exposure to suicide, emphasizing the benefits of receiving training, and using a university as the campaign messenger.

Conclusions
A social media campaign was a feasible and acceptable approach to recruiting and retaining participants—especially the loved ones of veterans with prior exposure to suicide—for a fully remote trial of SAVE gatekeeper training. Several campaign strategies may be applied to further promote remote study participation in this population.

Trial Registration
ClinicalTrials.gov NCT04565951; https://clinicaltrials.gov/ct2/show/NCT04565951

Conflicts of Interest
None declared.