bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeted sequencing analysis of Mycoplasma gallisepticum isolates in chicken layer and breeder flocks in Thailand
Authors: Limsatanun Arithat , Pakpinyo Somsak , Limpavithayakul Kriengwich , Prasertsee Teerarat 
Published: June 2022

AbstractMycoplasma gallisepticum(MG) is one of the most economically important pathogens worldwide. MG affects the respiratory system and impairs growth performance in poultry. In developing countries, the most widely used technique to identify MG is the conventional PCR assay. In this study, 24 MG isolates collected from Thailand farms with unvaccinated chickens during 2002–2020 were characterized by gene-targeted sequencing (GTS), followed by phylogenetic analysis using unweighted pair group method with arithmetic mean. These 24 Thai MG isolates differed from vaccine strains, including the F, ts-11 and 6/85 strains. One isolate showed 99.5–100% genetic similarity to the F strain with 4 partial gene analyses. This result may have been due to contamination from vaccinated flocks because the F strain is the most commonly used vaccine strain in Thailand. However, the GTS analysis using the partial MG genes in this study showed that the isolates could be grouped into different patterns based on individual gene sequences. The phylogenetic analysis of partialmgc2,gapA,pvpAandlpgene sequences classified the Thai MG isolates into 7, 11, 7 and 2 groups, respectively. In conclusion, at least 2 partial MG genes, especially partialgapA andmgc2 genes, are needed to differentiate MG isolates.
Remodeling of gene regulatory networks underlying thermogenic stimuli-induced adipose beiging
Authors: Lee Seoyeon , Benvie Abigail M. , Park Hui Gyu , Spektor Roman , Harlan Blaine , Brenna J. Thomas , Berry Daniel C. , Soloway Paul D. 
Published: June 2022

AbstractBeige adipocytes are induced by cold temperatures or β3-adrenergic receptor (Adrb3) agonists. They create heat through glucose and fatty acid (FA) oxidation, conferring metabolic benefits. The distinct and shared mechanisms by which these treatments induce beiging are unknown. Here, we perform single-nucleus assay for transposase-accessible chromatin sequencing (snATAC-seq) on adipose tissue from mice exposed to cold or an Adrb3 agonist to identify cellular and chromatin accessibility dynamics during beiging. Both stimuli induce chromatin remodeling that influence vascularization and inflammation in adipose. Beige adipocytes from cold-exposed mice have increased accessibility at genes regulating glycolytic processes, whereas Adrb3 activation increases cAMP responses. While both thermogenic stimuli increase accessibility at genes regulating thermogenesis, lipogenesis, and beige adipocyte development, the kinetics and magnitudes of the changes are distinct for the stimuli. Accessibility changes at lipogenic genes are linked to functional changes in lipid composition of adipose. Both stimuli tend to decrease the proportion of palmitic acids, a saturated FA in adipose. However, Adrb3 activation increases the proportion of monounsaturated FAs, whereas cold increases the proportion of polyunsaturated FAs. These findings reveal common and distinct mechanisms of cold and Adrb3 induced beige adipocyte biogenesis, and identify unique functional consequences of manipulating these pathways in vivo.
Simulated bifurcation assisted by thermal fluctuation
Authors: Kanao Taro , Goto Hayato 
Published: June 2022

AbstractVarious kinds of Ising machines based on unconventional computing have recently been developed for practically important combinatorial optimization. Among them, the machines implementing a heuristic algorithm called simulated bifurcation have achieved high performance, where Hamiltonian dynamics are simulated by massively parallel processing. To further improve the performance of simulated bifurcation, here we introduce thermal fluctuation to its dynamics relying on the Nosé–Hoover method, which has been used to simulate Hamiltonian dynamics at finite temperatures. We find that a heating process in the Nosé–Hoover method can assist simulated bifurcation to escape from local minima of the Ising problem, and hence lead to improved performance. We thus propose heated simulated bifurcation and demonstrate its performance improvement by numerically solving instances of the Ising problem with up to 2000 spin variables and all-to-all connectivity. Proposed heated simulated bifurcation is expected to be accelerated by parallel processing.
A tale of topoisomerases and the knotty genetic material in the backdrop of Plasmodium biology
Authors: Singh Priyanka , Rani Khushboo , Gotmare Akanksha , Bhattacharyya Sunanda 
Published: June 2022

Abstract
The untangling or overwinding of genetic material is an inevitable part of DNA replication, repair, recombination, and transcription. Topoisomerases belong to a conserved enzyme family that amends DNA topology during various processes of DNA metabolism. To relax the genetic material, topoisomerases transiently break the phosphodiester bond on one or both DNA strands and remain associated with the cleavage site by forming a covalent enzyme–DNA intermediate. This releases torsional stress and allows the broken DNA to be re-ligated by the enzyme. The biological function of topoisomerases ranges from the separation of sister chromatids following DNA replication to the aiding of chromosome condensation and segregation during mitosis. Topoisomerases are also actively involved in meiotic recombination. The unicellular apicomplexan parasite, Plasmodium falciparum, harbors different topoisomerase subtypes, some of which have substantially different sequences and functions from their human counterparts. This review highlights the biological function of each identified Plasmodium topoisomerase along with a comparative analysis of their orthologs in human or other model organisms. There is also a focus on recent advancements towards the development of topoisomerase chemical inhibitors, underscoring the druggability of unique topoisomerase subunits that are absent in humans. Plasmodium harbors three distinct genomes in the nucleus, apicoplast, and mitochondria, respectively, and undergoes non-canonical cell division during the schizont stage of development. This review emphasizes the specific developmental stages of Plasmodium on which future topoisomerase research should focus.
Practical distinguishing attack against the IoT‐friendly block cipher ALLPC
Authors: Cui Ting , Zhang Jiyan , Jin Chenhui , Chen Shiwei , Yang Yang 
Published: June 2022

AbstractThe issue of security and privacy plays an important role in the Internet of Things (IoT) and directly affects its wide applications. In order to meet the security requirements in the IoT environment, a series of lightweight encryption schemes have been proposed. Meanwhile, cryptanalysis against these schemes is critical to the security of IoT. In this paper, a practical distinguishing attack on an IoT‐friendly lightweight cipher ALLPC is presented. Specifically, it is found that there exists a fixed point in the differential of ALLPC's S‐box, then a differential trail with a period of 14‐round has been proposed. By connecting to the periodic differential trail, a differential trail is constructed for the full‐round ALLPC with a probability of 2−24. Applying such property, one can distinguish the full‐round ALLPC block cipher from a random permutation in about 10 min on a laptop.
Optical likelihood calculation for quadrature phase‐shift keying signal based on silicon integrated waveguide
Authors: Aikawa Yohei 
Published: June 2022

AbstractAll‐optical likelihood calculation has the potential to improve the performance of operating speed and power consumption in future communication systems. The author previously proposed a novel scheme of likelihood calculation, which is capable of applying multi‐value modulation. However, these studies have a limitation that requires higher integration into an actual system. In this study, an optical device of likelihood calculation for a 4‐bit quadrature phase‐shift keying (QPSK) modulated signal (i.e. two sequential QPSK symbols) is demonstrated. The integrated device consists of two delayed interferometers with silicon waveguide. The device is designed according to the types of 4‐bit code string as follows (00 00), (00 11), (11 00), and (11 11), and the output light waveform from the device is observed by an oscilloscope for a plurality of 4‐bit QPSK signals. The light intensity obtained from the device accurately corresponds to the Hamming distance between the code string and the input signal. The results indicate that the proposed scheme correctly calculates a likelihood for a 4‐bit QPSK signal at 10 Gbaud.
El peroné flotante: Resultado de una luxación tibioastragalina pura con lesión de la articulación tibioperonea proximal: Reporte de caso
Authors: Díez-Albero Luis , Saura-Sánchez Eladio 
Published: June 2022

Resumen
Antecedentes El peroné flotante tras un traumatismo de alta energía es una entidad muy poco frecuente. Este es el primer reporte de caso asociado a luxación del tendón bicipital.
Objetivo Presentar un caso de peroné flotante tras luxación divergente de tobillo asociado a luxación peronea proximal.
Materiales y métodos Mujer de 17 añ que, tras accidente de tráfico, sufrió caída y presentó dolor y deformidad del tobillo izquierdo, junto con dolor e impotencia funcional de la rodilla ipsilateral. En las radiografías, se apreció una luxación pura divergente del tobillo izquierdo de tipo IV. Se realizó reducción cerrada en urgencias. El estudio se complementó con una resonancia magnética que mostró una rotura completa de la sindesmosis y del ligamento deltoideo, así como una fractura no desplazada de la meseta tibial externa, junto con un desgarro de la cápsula tibioperonea proximal y desprendimiento completo del ligamento lateral externo y del tendón bicipital desde su inserción en la cabeza del peroné.Se realizó reanclaje de la sindesmosis y del ligamento deltoideo, así como del ligamento lateral externo y del tendón bicipital mediante anclajes óseos metálicos y reducción de peroné tanto proximal como distalmente, mediante sistema de sutura tipo botón. Se inmovilizó con férula cruropédica por cuatro semanas.
Resultados La paciente presentó recuperación completa de la fuerza a los cinco meses de seguimiento, con movilidad completa del tobillo y de la rodilla.
Conclusión El peroné flotante es muy poco frecuente; sólo se ha descrito un caso en la literatura. Implica la disrupción de la articulación tibioperonea proximal y distal, lo que puede provocar inestabilidad en la rodilla y en el tobillo.Por tanto, ante un traumatismo de alta energía en el tobillo, es necesaria la exploración minuciosa de la rodilla ipsilateral.
Prostate Artery Embolization in Patients above Eighty Years Old: Clinical Efficacy and Safety
Authors: Saro Hossam , Solyman M. Th. , Zaki Mohammed , Hasan M.A. N. , Thulasidasan N. , Clovis Sharon , Elhage Oussama , Popert Rick , Sabharwal Tarun 
Published: June 2022

Abstract
Objectives Prostate artery embolization (PAE) has been established as an effective treatment option for benign prostate hyperplasia or hematuria of prostatic origin. We aim to confirm the effectiveness and safety of PAE in elderly patients aged ≥ 80 years old.
Materials and Methods Between January 2014 and August 2020, PAE was attempted on 54 elderly patients with lower urinary tract symptoms (LUTS) or prostatic hematuria who were unfit for surgical treatment or opted for PAE. Outcome parameters (International Prostate Symptom Score [IPSS], quality of life [QoL] score, International Index of Erectile Function score (IIEF), maximal urinary flow rate, postvoid residual, and prostate volume) were collected and analyzed at baseline, 6 months, 1, 2, and 3 years.
Results The mean patient age was 85.29 years (range: 80–98). Technical success was achieved in 50 patients (92.6%). Mean IPSS improved from 18 at baseline to 7.7, 8.5, 8.6, and 9.1 at 6 months, 1, 2, and 3 years. Mean QoL improved from 4.9 at baseline to 2.8, 1.7, and 1.5 at 6 months, 1, and 2 years. Mean prostate volume reduced from a baseline of 152.7 to 123.5 mL within 6 months and 120.5 mL after 7 months of PAE. Urinary catheter removal was successful in 13 out of 19 patients with urinary retention. PAE succeeded in stopping bleeding in 16 out of 17 patients with prostate-induced hematuria.
Conclusion PAE is a feasible low-risk treatment for LUTS with or without urinary retention or prostatic hematuria in elderly patients.
Increase of Micro-vessels Beneath the Pleural Surface on CT Predicts Pleural Adhesions
Authors: Kawamura Tomoyuki , Kobayashi Naohiro , Goto Yukinobu , Sato Yukio 
Published: June 2022

Abstract
Background Pleural adhesions are often troublesome in lung surgeries. In some dense pleural adhesions, blood vessels between lung and chest wall (BVLC) are found during surgery. Theoretically, BVLC would increase the amount of blood flow just below the visceral pleura and could allow blood vessels beneath the pleural surface to be clearly visualized on CT. In this study, we investigated whether it was possible to identify the typical CT findings of cases with BVLC.
Methods Medical records and imaging findings of 186 patients who underwent surgery for lung tumors in our institution were retrospectively reviewed.
Results BVLC was found in 56 patients, of whom 44 (79%) had findings on preoperative CT that indicated increase of micro-vessels just below pleura. In the 21 patients with BVLC of ≥1 mm vessel diameter, the same CT findings were recognized in 19 cases (90%).
Conclusion Our results indicate that the CT finding of increased micro-vessels just below pleura has the potential to be used as a novel predictor of pleural adhesion.
Assessing soil quality and their indicators for long-term rice-based cropping systems in hot sub-humid eco-region of India
Authors: Kumar Uttam , Mishra V. N. , Kumar Nirmal , Srivastava L. K. , Tedia K. , Bajpai R. K. , Singh Muneshwar , Mohanty M. 
Published: June 2022

Context Rice-based cropping system is a key for food security in the Indian subcontinent. However, the productivity of such systems has declined continuously due to deterioration in soil quality. The present study was carried out to identify key soil quality indicators and to develop indices of soil quality under long-term rice-based cropping systems in the hot sub-humid eco-region (HSE) of India. Methods Surface soil samples were collected from farmers’ field from two soil orders (Vertisols and Inceptisols) and with four long-term rice-based cropping systems: (1) rice-wheat (RW); (2) rice-chickpea (RC); (3) rice-lathyrus (RL); and (4) rice-fallow (RF). A minimum data set (MDS) of 24 soils attributes were screened to identify soil quality indicators using principal components analysis (PCA), and soil quality index (SQI) was determined. Key results The MDS indicators (in descending order of sensitivity) are field capacity (FC) (35.4%) followed by pH (30.5%) > porosity (13.6%) > potentially mineralisable carbon (PMC) (11.8%) > available boron (AB) (8.7%). Soil moisture content under FC was found as the most sensitive indicator affecting soil quality and rice equivalent yields (REY). The SQI of Vertisols (0.83) was significantly higher (P < 0.05) than Inceptisols (0.73). SQI under RC (0.86) cropping systems was significantly higher than RL (0.81), RW (0.76) and RF (0.78) (P < 0.05). Conclusions The inclusion of legumes (chickpea and lathyrus) into cropping systems sustained better soil quality and productivity. Implications SQI is not only useful for assessing soil health but also provides indicators of best-bet management practices for sustaining intensive cultivation.
Repeated‐sprint training in heat and hypoxia: Acute responses to manipulating exercise‐to‐rest ratio
Authors: Dennis Myles C. , Goods Paul S.R. , Binnie Martyn J. , Girard Olivier , Wallman Karen E. , Dawson Brian , Billaut Francois , Peeling Peter 
Published: June 2022

ABSTRACTThe aim of this study was to investigate acute performance and physiological responses to the manipulation of exercise‐to‐rest ratio (E:R) during repeated‐sprint hypoxic training (RSH) in hot conditions. Twelve male team‐sport players completed two experimental sessions at a simulated altitude of ∼3000 m (FIO2 0.144), air temperature of 40°C and relative humidity of 50%. Exercise involved either 3 × 5 × 10‐s (E:R1:2) or 3 × 10 × 5‐s (E:R1:4) maximal cycling sprints interspersed with active recoveries at 120W (20‐s between sprints, 2.5 and 5‐min between sets for E:R1:2 and E:R1:4 respectively). Sessions were matched for overall sprint and total session duration (47.5‐min). Peak and mean power output, and total work were greater in E:R1:4 than E:R1:2 (p < 0.05). Peak core temperature was significantly higher in E:R1:4 than E:R1:2 (38.44 ± 0.33 vs. 38.20 ± 0.35°C, p = 0.028). Muscle deoxygenation magnitude during sprints was greater in E:R1:2 (28.2 ± 1.6 vs. 22.4 ± 4.6%, p < 0.001), while muscle reoxygenation did not differ between conditions (p > 0.05). These results indicate E:R1:4 increased mechanical power output and core temperature compared to E:R1:2. Both protocols had different effects on measures of muscle oxygenation, with E:R1:2 generating greater muscle oxygen extraction and E:R1:4 producing more muscle oxygenation flux, which are both important signals for peripheral adaptation. We conclude that the E:R manipulation during RSH in the heat might be used to target different physiological and performance outcomes, with these findings forming a strong base for future mechanistic investigation.
Online learning mediated by social teaching platforms: an experience from a flipped undergraduate physics course in renewable energies
Authors: Tuveri Matteo , Zurru Alessia , Fadda Daniela , Saba Michele 
Published: June 2022

Abstract
Active learning strategies and information technology-based pedagogies are perceived as beneficial and their use and development in academic courses is fast increasing. Faculties can use them to design courses that are challenging and fresher, creative, and meaningful for students, to meet students’ needs and to promote the learning of contents in a social environment, fostering cooperation and the exchange of ideas among peers. Among the active learning strategies recently developed, the peer-instruction and the flipped classroom are prominent in this field. It has been shown that these practices help students in their understanding of contents and consequently in reaching better results in final exam scores. The Covid-19 pandemic in 2020 and 2021 has forced classes in many academic institutions to move to remote teaching under emergency conditions and has deprived students of much of their social interactions. We show here an experience on applying online learning mediated by social teaching platforms and flipped classroom concepts to an undergraduate course on renewable energies provided amid the Covid-19 pandemic at the Physics Department of the University of Cagliari (Italy) in 2020. The constraints of forced distance learning have been turned into an opportunity to test an alternate course format in a complete online environment. An analysis on students’ feedback about our learning method has been performed by a post-diagnostic satisfaction questionnaire, indicating an overall positive effects of active learning strategies in the students’ learning experience. The analysis of their achievements during the course provides confirmation for the positive outcome and shows that the flipped classroom architecture is as robust as the traditional methods when facing the constraints of remote learning.
The Feynman paradox and hidden momentum
Authors: Jiménez J L , Campos I , Roa-Neri J A E 
Published: June 2022

Abstract
Though it is generally accepted that electromagnetic radiation fields possess energy and linear and angular momentum, the adscription of these properties to static and quasi-static fields rises some doubts. Some authors consider that in electrostatics the concept of field is superfluous, or that action-at-a-distance and field are equivalent views. However, the interpretation of Poynting’s vector divided by c
2 as a momentum density is consistent and necessary for the conservation laws to hold, even in static conditions. The Feynman paradox exhibits the necessity of assigning linear and angular momentum to static and quasi-static fields, showing in this way that the points of view of action-at-a-distance and field are not equivalent. In the present work we do not intend to present a revision of the literature on this subject, but to show that the concept of hidden momentum is useful to solve the paradox, and we show that it is part of a momentum balance equation derived directly from the macroscopic Maxwell equations. Another consequence of this balance equation is that, in static conditions, the momentum associated to Poynting’s vector equals the hidden momentum and cancels it. Graduate students and researchers interested in conceptual problems of electromagnetism will find a firm foundation of the concept of hidden momentum and an alternative view of the paradox.
Measuring the refractive index of a transparent sphere from the primary and secondary rainbows
Authors: Ma Yao , Lin Hao-Chen , Shi Wen-Jia , Zhang Qin , Qu Guang-Hui , Pang Mi 
Published: June 2022

Abstract
We propose a method to obtain the refractive index of a transparent sphere from the fascinating phenomena of rainbows, which can be used as an interesting subject in the experimental courses of primary optics for undergraduate or high school students. The primary and secondary rainbows are reproduced on white walls and screens in the classroom with the sun and water drops replaced by the LED flashlight and K9 glass (one type of borosilicate glass) sphere respectively. The locations of the primary and secondary rainbows on the screen are measured, with the locations of the screen and sphere changed. By fitting the multiple measurements to the theoretical result using the least square method, the refractive index of the glass sphere, as an unknown parameter in the fitting process, is obtained. For both the primary and secondary rainbows, the locations of the red, yellow and purple bows are measured independently. Repetitive measurements give very stable and accurate output with the standard deviation ratio as low as 10−4 and the relative error all around 0.1% compared to the result obtained from special instruments in the literature. The fascinating phenomena and precise measurements enable this method to fully deepen the students’ understanding of the law of refraction and the application of the least square method.