bluuebunny/crossref_metadata_2025_split huggingface dataset:
Farmers' preferences for sustainable intensification attributes in sorghum-based cropping systems: evidence from Mali
Authors: Badolo Felix , Kotu Bekele Hundie , Oyinbo Oyakhilomen , Sanogo Karamoko , Birhanu Birhanu Zemadim 
Published: November 2022

AbstractSorghum plays a crucial role in the rural economy and nutrition of rural households in Mali. Yet the productivity of this crop is constrained by limited adoption of agricultural intensification technologies, which could be partly because technology development does not properly consider farmers' preferences. This study with smallholder farmers in southern Mali aimed to assess farmers' preferences for different attributes of sorghum technologies through the lens of sustainable intensification. The study used a discrete choice experiment, a method which involves asking individuals to state their preference over hypothetical alternative scenarios, goods or services. We considered six attributes corresponding to different domains of sustainable intensification: grain yield, risk of yield loss, soil fertility, nutrition, labor requirement and fodder yield. We analyzed the data using the mixed logit model, while considering the multinomial logit model as a robustness check. The findings revealed that smallholder farmers are strongly interested in transitioning from their existing sorghum-based cropping systems to those that closely align with these domains of sustainable intensification. However, there were diverse preferences among all the smallholder farmers studied, and between distinct sub-groups of smallholder farmers characterized by their social networks and agroecological zones, which yield relevant policy implications. Overall, these results support the growing research and development prioritization and policy interests toward scaling sustainable intensification among farmers, with a particular focus on human nutrition.
Political parties, issue salience, and the appointment of women cabinet members
Authors: Höhmann Daniel 
Published: November 2022

AbstractDo parties relegate female ministers to portfolios that are politically less important for them? This research note contributes to this debate and examines whether the issue salience of parties for specific policy areas has an effect on the nomination of a female minister. Previous theoretical work assumes that party leaders will be more likely to select men for those portfolios that are highly salient for the party. To test this assumption empirically, the paper analyzes the appointment of women cabinet members in the German states between 2006 and 2021. Notably, the findings contradict the theoretical expectations as well as previous empirical results from a cross-national study: On the German sub-national level the nomination of a female minister is more likely if the respective portfolio is highly salient for the governing party. Parties and their policy-preferences seem to be an important factor in explaining the share of women in sub-national cabinets.
High Spatial Variability of Biomass Burning Emissions Observed at Three Tibetan Plateau Sites
Authors: Zhu Chong‐Shu , Qu Yao , Zhang Zhi‐Sheng , Zhang Ting , Dai Wen‐Ting , Cao Jun‐Ji 
Published: November 2022

AbstractAerosol from biomass burning (BB) can affect regional and global climate by changing radiation balance and cloud properties, especially over the high altitude environ‐vulnerable region. To investigate the contributions of BB on atmospheric particulate matter trans‐the Tibetan Plateau (TP), the total suspended particle samples were collected at three high altitude sites over the northeastern (Qinghai lake, 3,200 m a.s.l.), central (Beiluhe, 4,659 m a.s.l.), and southwestern TP (Ngari, 4,360 m a.s.l.), respectively. Highly spatial variations of BB tracers (including levoglucosan, mannosan, and galactosan) were obtained. Elevated BB tracer concentrations were observed in the northeastern and southwestern sites compared to the central TP site. The heighten concentrations of particulate matter from biomass burning and organic carbon from biomass burning were obtained at Ngari (with 5260.3 and 2257.7 ng m−3) and Qinghai lake (with 1508.8 and 647.6 ng m−3) compared with those at Beiluhe (with 18.8 and 8.1 ng m−3), respectively. The results indicated that the BB contributions to aerosol were significant on the margin of the TP. In addition to the long‐range transport of BB pollutant, the local emissions may be an important contributor in the northeastern and southwestern TP. The present study can improve the understanding of BB characteristics and effects in the TP.
Toward More Actionable Flood‐Risk Information
Authors: Cooper C. M. , Sharma S. , Nicholas R. E. , Keller K. 
Published: November 2022

AbstractThe increasingly urgent need to develop knowledge and practices to manage flood risks drives innovative information design. However, experts often disagree about design practices. As a result, flood‐risk estimates can diverge, leading to different conclusions for decision‐making. Using examples of household‐scale fluvial (riverine) flood‐risk information in the United States, we assess design features and risk communication approaches that may lead to more actionable information for decision‐making. We argue that increased attention to uncertainty characterization and model diagnostics is a critical intermediate step for developing simpler approaches for designing flood‐risk information. Simpler frameworks are desirable because flood risks change over time, and simpler frameworks are less costly to update. Developing frameworks for large spatial domains require collaboration grounded in principles of open science. Finally, systematically evaluating how decision‐makers access and use information can provide new insights to guide risk communication and information design.
The Shortwave Radiative Flux Response to an Injection of Sea Salt Aerosols in the Gulf of Mexico
Authors: Goddard P. B. , Kravitz B. , MacMartin D. G. , Wang H. 
Published: November 2022

AbstractMarine cloud brightening (MCB) has been proposed as a potential means of geoengineering the climate, temporarily providing cooling to offset some of the effects of climate change. Marine sky brightening (MSB), involving the direct scattering of sunlight from sea salt injection into the marine boundary layer, has been proposed as an additional geoengineering method that could work in areas that are not regularly cloudy. Here, we use a regional atmospheric model to simulate MCB and MSB over the Gulf of Mexico and nearby land, a highly populated and economically important region that is not characterized by persistent marine stratocumulus cloud cover. Injection of sea salt in the Aitken mode from a region in the central Gulf of Mexico equivalent to 10.8 Tg yr−1 produces an upwards 8.4 W m−2 radiative flux change across the region at the top of the atmosphere, largely due to cloud property changes. Comparatively, a similar mass injection in the accumulation mode produces a 3.1 W m−2 radiative flux change driven primarily by direct scattering. Injection of even larger particles produces a much smaller radiative flux change. Shortwave flux changes due to clouds are largely driven by an increase in cloud droplet number concentration and an increase in cloud liquid water path (each contributing about 45% to the flux change), with a much lower contribution from cloud fraction changes (10%).
Maternal stress and vulnerability to depression: coping and maternal care strategies and its consequences on adolescent offspring
Authors: Alves Renata L. , Portugal Camila C. , Lopes Igor M. , Oliveira Pedro , Alves Cecília J. , Barbosa Fernando , Summavielle Teresa , Magalhães Ana 
Published: November 2022

AbstractDepressive mothers often find mother-child interaction to be challenging. Maternal stress may further impair mother-child attachment, which may increase the risk of negative developmental consequences. We used rats with different vulnerability to depressive-like behavior (Wistar and Kyoto) to investigate the impact of stress (maternal separation-MS) on maternal behavior and adolescent offspring cognition. MS in Kyoto dams increased pup-contact, resulting in higher oxytocin levels and lower anxiety-like behavior after weaning, while worsening their adolescent offspring cognitive behavior. Whereas MS in Wistar dams elicited higher quality of pup-directed behavior, increasing brain-derived neurotrophic factor (BDNF) in the offspring, which seems to have prevented a negative impact on cognition. Hypothalamic oxytocin seems to affect the salience of the social environment cues (negatively for Kyoto) leading to different coping strategies. Our findings highlight the importance of contextual and individual factors in the understanding of the oxytocin role in modulating maternal behavior and stress regulatory processes.
Effect of human mesenchymal stem cell secretome administration on morphine self-administration and relapse in two animal models of opioid dependence
Authors: Quintanilla María Elena , Quezada Mauricio , Morales Paola , Berríos-Cárcamo Pablo , Santapau Daniela , Ezquer Marcelo , Herrera-Marschitz Mario , Israel Yedy , Ezquer Fernando 
Published: November 2022

AbstractThe present study investigates the possible therapeutic effects of human mesenchymal stem cell-derived secretome on morphine dependence and relapse. This was studied in a new model of chronic voluntary morphine intake in Wistar rats which shows classic signs of morphine intoxication and a severe naloxone-induced withdrawal syndrome. A single intranasal-systemic administration of MSCs secretome fully inhibited (>95%; p < 0.001) voluntary morphine intake and reduced the post-deprivation relapse intake by 50% (p < 0.02). Since several studies suggest a significant genetic contribution to the chronic use of many addictive drugs, the effect of MSCs secretome on morphine self-administration was further studied in rats bred as high alcohol consumers (UChB rats). Sub-chronic intraperitoneal administration of morphine before access to increasing concentrations of morphine solutions and water were available to the animals, led UChB rats to prefer ingesting morphine solutions over water, attaining levels of oral morphine intake in the range of those in the Wistar model. Intranasally administered MSCs secretome to UChB rats dose-dependently inhibited morphine self-administration by 72% (p < 0.001); while a single intranasal dose of MSC-secretome administered during a morphine deprivation period imposed on chronic morphine consumer UChB rats inhibited re-access morphine relapse intake by 80 to 85% (p < 0.0001). Both in the Wistar and the UChB rat models, MSCs-secretome administration reversed the morphine-induced increases in brain oxidative stress and neuroinflammation, considered as key engines perpetuating drug relapse. Overall, present preclinical studies suggest that products secreted by human mesenchymal stem cells may be of value in the treatment of opioid addiction.
Super-resolution microscopy enabled by high-efficiency surface-migration emission depletion
Authors: Pu Rui , Zhan Qiuqiang , Peng Xingyun , Liu Siying , Guo Xin , Liang Liangliang , Qin Xian , Zhao Ziqing Winston , Liu Xiaogang 
Published: November 2022

AbstractNonlinear depletion of fluorescence states by stimulated emission constitutes the basis of stimulated emission depletion (STED) microscopy. Despite significant efforts over the past decade, achieving super-resolution at low saturation intensities by STED remains a major technical challenge. By harnessing the surface quenching effect in NaGdF4:Yb/Tm nanocrystals, we report here high-efficiency emission depletion through surface migration. Using a dual-beam, continuous-wave laser manipulation scheme (975-nm excitation and 730-nm de-excitation), we achieved an emission depletion efficiency of over 95% and a low saturation intensity of 18.3 kW cm−2. Emission depletion by surface migration through gadolinium sublattices enables super-resolution imaging with sub-20 nm lateral resolution. Our approach circumvents the fundamental limitation of high-intensity STED microscopy, providing autofluorescence-free, re-excitation-background-free imaging with a saturation intensity over three orders of magnitude lower than conventional fluorophores. We also demonstrated super-resolution imaging of actin filaments in Hela cells labeled with 8-nm nanoparticles. Combined with the highly photostable lanthanide luminescence, surface-migration emission depletion (SMED) could provide a powerful mechanism for low-power, super-resolution imaging or biological tracking as well as super-resolved optical sensing/writing and lithography.
Retinal chromophore charge delocalization and confinement explain the extreme photophysics of Neorhodopsin
Authors: Palombo Riccardo , Barneschi Leonardo , Pedraza-González Laura , Padula Daniele , Schapiro Igor , Olivucci Massimo 
Published: November 2022

AbstractThe understanding of how the rhodopsin sequence can be modified to exactly modulate the spectroscopic properties of its retinal chromophore, is a prerequisite for the rational design of more effective optogenetic tools. One key problem is that of establishing the rules to be satisfied for achieving highly fluorescent rhodopsins with a near infrared absorption. In the present paper we use multi-configurational quantum chemistry to construct a computer model of a recently discovered natural rhodopsin, Neorhodopsin, displaying exactly such properties. We show that the model, that successfully replicates the relevant experimental observables, unveils a geometrical and electronic structure of the chromophore featuring a highly diffuse charge distribution along its conjugated chain. The same model reveals that a charge confinement process occurring along the chromophore excited state isomerization coordinate, is the primary cause of the observed fluorescence enhancement.
The contribution of common and rare genetic variants to variation in metabolic traits in 288,137 East Asians
Authors: Kim Young Jin , Moon Sanghoon , Hwang Mi Yeong , Han Sohee , Jang Hye-Mi , Kong Jinhwa , Shin Dong Mun , Yoon Kyungheon , Kim Sung Min , Lee Jong-Eun , Mahajan Anubha , Park Hyun-Young , McCarthy Mark I. , Cho Yoon Shin , Kim Bong-Jo 
Published: November 2022

AbstractMetabolic traits are heritable phenotypes widely-used in assessing the risk of various diseases. We conduct a genome-wide association analysis (GWAS) of nine metabolic traits (including glycemic, lipid, liver enzyme levels) in 125,872 Korean subjects genotyped with the Korea Biobank Array. Following meta-analysis with GWAS from Biobank Japan identify 144 novel signals (MAF ≥ 1%), of which 57.0% are replicated in UK Biobank. Additionally, we discover 66 rare (MAF < 1%) variants, 94.4% of them co-incident to common loci, adding to allelic series. Although rare variants have limited contribution to overall trait variance, these lead, in carriers, substantial loss of predictive accuracy from polygenic predictions of disease risk from common variant alone. We capture groups with up to 16-fold variation in type 2 diabetes (T2D) prevalence by integration of genetic risk scores of fasting plasma glucose and T2D and the I349F rare protective variant. This study highlights the need to consider the joint contribution of both common and rare variants on inherited risk of metabolic traits and related diseases.
Atomic-level structural responsiveness to environmental conditions from 3D electron diffraction
Authors: Ling Yang , Sun Tu , Guo Linshuo , Si Xiaomeng , Jiang Yilan , Zhang Qing , Chen Zhaoxi , Terasaki Osamu , Ma Yanhang 
Published: November 2022

AbstractElectron microscopy has been widely used in the structural analysis of proteins, pharmaceutical products, and various functional materials in the past decades. However, one fact is often overlooked that the crystal structure might be sensitive to external environments and response manners, which will bring uncertainty to the structure determination and structure-property correlation. Here, we report the atomic-level ab initio structure determinations of microcrystals by combining 3D electron diffraction (3D ED) and environmental transmission electron microscope (TEM). Environmental conditions, including cryo, heating, gas and liquid, have been successfully achieved using in situ holders to reveal the simuli-responsive structures of crystals. Remarkable structural changes have been directly resolved by 3D ED in one flexible metal-organic framework, MIL-53, owing to the response of framework to pressures, temperatures, guest molecules, etc.
Amplification of integrated microscopic motions of high-density [2]rotaxanes in mechanically interlocked networks
Authors: Yang Xue , Cheng Lin , Zhang Zhaoming , Zhao Jun , Bai Ruixue , Guo Zhewen , Yu Wei , Yan Xuzhou 
Published: November 2022

AbstractIntegrating individual microscopic motion to perform tasks in macroscopic sale is common in living organisms. However, developing artificial materials in which molecular-level motions could be amplified to behave macroscopically is still challenging. Herein, we present a class of mechanically interlocked networks (MINs) carrying densely rotaxanated backbones as a model system to understand macroscopic mechanical properties stemmed from the integration and amplification of intramolecular motion of the embedded [2]rotaxane motifs. On the one hand, the motion of mechanical bonds introduces the original dangling chains into the network, and the synergy of numerous such microscopic motions leads to an expansion of entire network, imparting good stretchability and puncture resistance to the MINs. On the other hand, the dissociation of host−guest recognition and subsequent sliding motion represent a peculiar energy dissipation pathway, whose integration and amplification result in the bulk materials with favorable toughness and damping capacity. Thereinto, we develop a continuous stress-relaxation method to elucidate the microscopic motion of [2]rotaxane units, which contributes to the understanding of the relationship between cumulative microscopic motions and amplified macroscopic mechanical performance.
Defect free strain relaxation of microcrystals on mesoporous patterned silicon
Authors: Heintz Alexandre , Ilahi Bouraoui , Pofelski Alexandre , Botton Gianluigi , Patriarche Gilles , Barzaghi Andrea , Fafard Simon , Arès Richard , Isella Giovanni , Boucherif Abderraouf 
Published: November 2022

AbstractA perfectly compliant substrate would allow the monolithic integration of high-quality semiconductor materials such as Ge and III-V on Silicon (Si) substrate, enabling novel functionalities on the well-established low-cost Si technology platform. Here, we demonstrate a compliant Si substrate allowing defect-free epitaxial growth of lattice mismatched materials. The method is based on the deep patterning of the Si substrate to form micrometer-scale pillars and subsequent electrochemical porosification. The investigation of the epitaxial Ge crystalline quality by X-ray diffraction, transmission electron microscopy and etch-pits counting demonstrates the full elastic relaxation of defect-free microcrystals. The achievement of dislocation free heteroepitaxy relies on the interplay between elastic deformation of the porous micropillars, set under stress by the lattice mismatch between Ge and Si, and on the diffusion of Ge into the mesoporous patterned substrate attenuating the mismatch strain at the Ge/Si interface.
Water-stable porous Al24 Archimedean solids for removal of trace iodine
Authors: Liu Ya-Jie , Sun Yi-Fan , Shen Si-Hao , Wang San-Tai , Liu Zhuang-Hua , Fang Wei-Hui , Wright Dominic S. , Zhang Jian 
Published: November 2022

AbstractIn this paper, we report a unique type of core-shell crystalline material that combines an inorganic zeolitic cage structure with a macrocyclic host arrangement and that can remove trace levels of iodine from water effectively. These unique assemblies are made up of an inorganic Archimedean truncatedhexahedron (tcu) polyhedron in the kernel which possesses six calixarene-like shell cavities. The cages have good adaptability to guests and can be assembled into a series of supramolecular structures in the crystalline state with different lattice pore shapes. Due to the unique core-shell porous structures, the compounds are not only stable in organic solvents but also in water. The characteristics of the cages enable rapid iodine capture from low concentration aqueous I2/KI solutions (down to 4 ppm concentration). We have studied the detailed process and mechanism of iodine capture and aggregation at the molecular level. The facile synthesis, considerable adsorption capacity, recyclability, and β- and γ-radiation resistance of the cages should make these materials suitable for the extraction of iodine from aqueous effluent streams (most obviously, radioactive iodide produced by atomic power generation).
A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages
Authors: Sun Pengkai , Zhang Zhenxing , Wang Bin , Liu Caiyun , Chen Chao , Liu Ping , Li Xinjian 
Published: November 2022

AbstractItaconate is a newly discovered endogenous metabolite promoting an anti-inflammatory program during innate immune response, but the precise mechanisms underlying its effect remains poorly understood owing primarily to the limitations of available itaconate-monitoring techniques. Here, we develop and validate a genetically encoded fluorescent itaconate biosensor, BioITA, for directly monitoring itaconate dynamics in subcellular compartments of living macrophages. Utilizing BioITA, we monitor the itaconate dynamics in response to lipopolysaccharide (LPS) stimulation in the context of modulating itaconate transportation and metabolism. Moreover, we show that STING activation induces itaconate production, and injection of AAVs expressing cytosolic BioITA into mice allows directly reporting elevation of itaconate level in activated macrophages derived from LPS-injected mice. Thus, BioITA enables subcellular resolution imaging of itaconate in living macrophages.
Mitochondrial Fission Process 1 controls inner membrane integrity and protects against heart failure
Authors: Donnarumma Erminia , Kohlhaas Michael , Vimont Elodie , Kornobis Etienne , Chaze Thibault , Gianetto Quentin Giai , Matondo Mariette , Moya-Nilges Maryse , Maack Christoph , Wai Timothy 
Published: November 2022

AbstractMitochondria are paramount to the metabolism and survival of cardiomyocytes. Here we show that Mitochondrial Fission Process 1 (MTFP1) is an inner mitochondrial membrane (IMM) protein that is dispensable for mitochondrial division yet essential for cardiac structure and function. Constitutive knockout of cardiomyocyte MTFP1 in mice resulted in a fatal, adult-onset dilated cardiomyopathy accompanied by extensive mitochondrial and cardiac remodeling during the transition to heart failure. Prior to the onset of disease, knockout cardiac mitochondria displayed specific IMM defects: futile proton leak dependent upon the adenine nucleotide translocase and an increased sensitivity to the opening of the mitochondrial permeability transition pore, with which MTFP1 physically and genetically interacts. Collectively, our data reveal new functions of MTFP1 in the control of bioenergetic efficiency and cell death sensitivity and define its importance in preventing pathogenic cardiac remodeling.