bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular docking of phytosterols in Stenochlaena palustris as anti-breast cancer
Authors: Marisa Dona , Hayatie Lisda , Juliati Siti , Suhartono Eko , Komari Noer 
Published: September 2022

Background: Stenochlaena palustris, also known as kelakai or lemidi, is frequently linked to anti-inflammatory, anti-bacterial, anti-fungal, and antioxidant properties. S. palustris phytosterols are suggested to suppress the progression of breast cancer.
Objective: The objective of this study is to evaluate the potential of phytosterols found in S. palustris to act as estrogen receptor (ER) inhibitors.
Methods: Phytosterols (alpha-tocopherol, beta-sitosterol, campesterol, stigmasterol, fucosterol) were docked to estrogen receptor (PDB ID: 7KBS). Molecular docking parameters included Gibb's free energy and interactions between ligand and protein. ADMET properties were analyzed using pkCSM and SwissADME.
Results: Alpha-tocopherol showed the highest interaction with the estrogen receptor with ΔG value -8. 9254 kcal/mol (the native ligand, raloxifene, had a G value of -12.052 kcal/mol). Leu387 (hydrogen bond); Phe404 (Phi-phi-T shaped), Leu391, Leu346, Trp383, Leu354, Ala350, Leu525, Leu349 (Alkyl) were among the residues by which a-tocopherol interacted with ER. Alpha-tocopherol has no hepatotoxicity and no skin sensitization.
Conclusion: By suppressing ERa, phytosterols from S. palustris may have potential anti-breast cancer activity and may be used to prevent estrogen-dependent human cancers like breast cancer.
PENERAPAN MODEL EXPERIENTIAL LEARNING UNTUK MENINGKATKAN TANGGUNG JAWAB DAN PRESTASI BELAJAR (Studi Pada Mata Pelajaran Bahasa Indonesia Kelas VIII SMP Negeri 5 Lahat)
Authors: Rusmilawati Rusmilawati 
Published: September 2022

This study aims to describe the application of experiential learning models in class VIII of SMP Negeri 5 Lahat. The method used in this study is a combined research method (mixed method research) which is a combination of action research and action research. The research subjects are students of SMP Negeri 5 Lahat in the first semester of 2019/2020 school year. The number of students for the class ptk 32 people, for the experiment 31 people, and for the control class 30 people. Data collection methods in this study use the assessment sheet and test results. Data analysis using score analysis and test tests consisting of different test between cycles difference test of two unrelated samples. The results showed that the application of the experimental learning model could increase responsibility and learning achievement in Indonesian VIII grade subjects at SMP Negeri 5 Lahat.
PENERAPAN METODE PEMBELAJARAN DRILL AND PRACTICE UNTUK MENINGKATKAN KEMAMPUAN SPEAKING DAN VOCABULARY (Studi Pada Mata Pelajaran Bahasa Inggris Kelas XI) SMK Negeri 2 Lahat
Authors: Hastenti Wimbika 
Published: September 2022

The research purpose is to describe the application of Drill and Practice learning methods to improve the speaking and vocabulary skills of students of class XI OTKP SMK Negeri 2 Lahat. The study design was the Classroom Action Research (CAR) model of Kemmis and Taggart, which was followed by a quasi experiment. The research subjects were students of class XI OTKP 1 even semester of the 2019/2020 school year SMK Negeri 2 Lahat. For quasi-experimental samples are class XI OTKP 1 and OTKP 3. Classroom action research totaling 25 students, for the experimental class totaling 25 students and the control class totaling 25 students. Data collection techniques in this research used a research sheet. Data analysis uses mean (percentage) percentages and t-tests. The conclusion of this research indicate that the application of the Drill And Practice method can improve students' speaking and vocabulary abilities.
PENGEMBANGAN SISTEM PENGUJIAN MENGUNAKAN MOODLE UNTUK MENINGKATKAN EFEKTIFITAS DAN EFISIENSI UJIAN HARIAN (Studi pada mata pelajaran Simulasi digital pada Kelas X di SMK Kota Bengkulu)
Authors: Putra Erawan , Alexon Alexon 
Published: September 2022

This study aims to describe,the development of testing system by usig moodleto improve the effeectiveness and efficiency of the daily examinations Digital Simulation sibjects. This research uses Research and Development (R & D) research with Borg & Gall development model in class X TKJ Bengkulu city, SMKN 3 Bengkulu city, SMKN 4 Bengkulu city. This study used steps of prelminary research, initial information collecting, planning, initial product format development, initial trials, product revisions, field trials , product revisions, preliminary form of product, preliminary field testing, main product revisions, field trials, refinement of final products and implementation. The instruments used questionnaires, observation sheets expert judgment questionmaires and test questions the data analysis technique used the percentage and t - test with the SPSS peogram. The results showed that moodle – based tet material development can improve the effectivenees and effeiciency of daily exams.
PENERAPAN MODEL PEMBELAJARAN KOOPERATIF TIPE TALKING STICK UNTUK MENINGKATKAN KETERAMPILAN BERBICARA DAN PRESTASI BELAJAR SISWA
Authors: Ariani Elida , Kurniah Nina 
Published: September 2022

This research is conducted to describe the application of talking stick type of cooperative learning model to increase the speaking skill and the students’ achievement at English Lesson of the tenthgrade at SMA Negeri 1 Kikim Timur. The research methods that used were Classroom Action Research and Quasi Experiment. The subjects of this research were students of the tenth grade SMA Negeri 1 Kikim Timur at the second semester of the academic year 2020/2021,for CAR class was X IPS 1,Experiment class X IPS 3,and Control Class X IPS 2. Each class totaling 27 students. The Research instruments used observation sheet and test. The data analysis used the average score analysis and test consisting of inter-cycle different test and unrelated two sample different test. The result showed that the application of cooperative type of learning model talking stick can increase the speaking skill and the students’ achievement of the tenthgradestudents of English lesson at SMA Negeri 1 Kikim Timur
Hepatic arterial infusion chemotherapy versus transarterial chemoembolization for unresectable hepatocellular carcinoma: A systematic review with meta-analysis
Authors: Si Tengfei , Huang Zhenlin , Khorsandi Shirin Elizabeth , Ma Yun , Heaton Nigel 
Published: September 2022

Background: Interest has revived in the use of hepatic arterial infusion chemotherapy (HAIC) for intermediate-advanced hepatocellular carcinoma (HCC) while transarterial chemoembolization (TACE) has been a longstanding loco-regional therapy.Aim: We conducted a systematic review and meta-analysis of patients with unresectable HCC treated with HAIC or TACE to look for differences in survival, adverse events, mortality and downstaging.Methods: All studies published before 29 July 2022 were identified by searching PubMed, Embase, Web of Science and Cochrane Library databases for patients with unresectable HCC and received HAIC or TACE as initial treatment. Data extracted from studies was statistically analysed using RevMan5.3 software.Results: A total of one randomized controlled trial (RCT) and 7 cohort studies (5 retrospective, 2 prospective) including 1,060 (TACE group: 534, HAIC group: 526) patients were screened. Compared with the TACE group, patients who received HAIC as initial therapy had better overall survival (OS) (HR = 0.53, 95%CI [0.40, 0.69]) and progression-free survival (PFS) (HR = 0.54, 95%CI [0.40, 0.72]). Further subgroup analysis revealed that HAIC showed priority over TACE on prognosis outcome regardless of tumour stage, especially in patients with advanced portal vein tumour thrombus (PVTT). Utilization of port system will not boost the efficacy of HAIC whereas using a replaced-microcatheter for each procedure could better reduce the progressive disease (PD) rate (RR = 0.55, 95%CI [0.40, 0.76]). The pooled RR favoured the HAIC group with regard to partial response (PR) (RR = 2.87, 95%CI [2.18, 3.78]) and this was validated by both GRADE summary and trial sequential analysis. The rate of resection after treatment was higher in the HAIC group (RR = 2.37, 95%CI [1.54, 3.66]), whilst no difference was found with procedure-related mortality (RR = 0.56, 95%CI [0.13, 2.38]) between two groups. Compared with the traditional chemotherapy regimen (fluorouracil/leucovorin/oxaliplatin) FOLFOX-HAIC appears to be better in improving the treatment efficacy.Conclusion: Patients with unresectable HCC could potentially benefit more from HAIC rather than standard TACE treatment. A re-evaluation of HAIC as a treatment option in intermediate and advanced HCC is warranted.
Application of near-infrared fluorescence imaging in lingual lymph node screening and drainage pattern observation for tongue cancer
Authors: Zhu Yaoping , Xiao Tao , He Yijia , Hong Xiaowei , Zhou Ting , Da Mingjie , Ge Sumeng , Xie Diya , Wang Zhiyong 
Published: September 2022

Objective: Lingual lymph node (LLN) metastasis is regarded as an indicator of unfavorable prognosis and a crucial sign of the high degree of primary tumor aggressiveness. However, detecting LLN metastasis is an important but frequently overlooked aspect of diagnosis and surgical treatment planning. The study aims to identify LLNs by intraoperative near-infrared (NIR) fluorescence imaging with indocyanine green absorbed into human serum albumin (ICG: HSA) and describe the presence of lymphatic drainage channels from the floor of the mouth in patients with tongue carcinoma.Materials and Methods: 21 patients diagnosed with cT1-T4 squamous cell carcinoma (SCC) of the tongue margin and scheduled to undergo tumor resection and unilateral neck dissection were enrolled. After exposing the neck, the patients were injected with 0.3 ml of ICG: HSA (500 μM) in three quadrants around the tumor, excluding the mucous membrane of the basal region cavity. Employing a near-infrared fluorescence imaging system, the fluorescence of levels I, II, III, and IV was measured during neck dissection.Results: LLNs were detected in four patients and were identified as metastatic LLNs in all 21 patients. The near-infrared fluorescence imaging system showed the existence of lymphatic drainage channels in the floor of the mouth. In patients receiving peritumoral injection of ICG: HSA, the mean fluorescence intensity (MFI)of metastatic lymph nodes (LNs) (178.4 ± 64.39, mean ± SD) was higher than that in non-metastatic LNs (132.0 ± 76.5, mean ± SD) (p &lt; 0.05).Conclusion: NIR fluorescence imaging with ICG: HSA could be used for intraoperative identification of LLNs and assist in the determination of metastatic lymph nodes for tongue carcinoma patients. Additionally, this finding demonstrates the feasibility of near-infrared fluorescence imaging in defining lymphatic drainage channels in the head and neck.
Improved solid-phase microextraction extraction procedure to detect trace-level epichlorohydrin in municipal water systems by HS-SPME-GC/MS
Authors: Lei Ping , Wang Lu , Yan Yun , Deng Wubin , Gao Jingsi , Zhu Jia , Liang Miaoqing , Wen Jiaheng , Lv Jianfeng , Zhou Jianfeng 
Published: September 2022

Epichlorohydrin (ECH) is toxic to humans via multiple routes and is a potential carcinogen. The accurate measurement of ECH at trace level (&lt;0.1 μg/L) is still an obstacle hindering the monitoring and regulation of municipal water systems. In this study, an improved headspace solid-phase microextraction (HS-SPME) procedure is developed and optimized to extract and enrich ECH with high sensitivity, accuracy, and precision. A total 17.4-time enhancement in extraction efficiency is achieved compared with the default condition. Specifically, the AC/PDMS/DVB fiber offered a 4.4-time enhancement comparing with the PDMS/DVB fiber. The effects of different mineral salts in SPME were studied and it was found that an addition of 3 g Na₂SO₄ in the SPME head achieved an additional 3.3-time increase. The pattern how sodium sulfate enhanced ECH extraction by salting out is discussed. The optimization of extraction conditions (pH = 7, 35°C, and 20 min extraction duration) brought another 1.2 times further. Combined with gas chromatography with mass spectrometry, the optimized method exhibits curve linearity in the range of 0.02–1.00 μg/L with an R2 of 0.998. The limit of detection, precision, and accuracy of the method are 0.006 μg/L, 2.6%–5.3%, and −3.5% to −2.0%, respectively. The recovery of ECH spiking in tap water and surface water was investigated, with recovery rates of 88.0%–116% and 72.5%–108%, respectively. Adhering to the requirements of existing water quality regulations, our method shows a high potential to be applied in drinking water quality monitoring and water treatment process assessment.
Synthesis and characterization of new 1,4-dihydropyran derivatives by novel Ta-MOF nanostructures as reusable nanocatalyst with antimicrobial activity
Authors: Ahmad Irfan , Jasim Saade Abdalkareem , Yasin Ghulam , Al-Qargholi Basim , Hammid Ali Thaeer 
Published: September 2022

Novel Ta- MOF was synthesized under mild conditions by ultrasound irradiations. The sample was characterized by SEM, FTIR, XRD, XPS, TG and BET technique. The final structures showed high physicho-chemical properties including narrow particle size distribution, homogenous morphology, high thermal stability and remarkable surface area. Ta- MOF synthesized in this study was used as a catalyst in the synthesis of 1,4-dihydropyran derivatives. The results proved that it has a high catalyst capability. Its advantages include high recyclability, less reaction time with higher efficiency and synthesis of new1,4-dihydropyran derivatives. In the following, antimicrobial activity including antifungal and antibacterial activity of Ta- MOF nanoparticles based on Minimum Inhibitory Concentration, Minimum Fungicidal Concentration and Minimum Bactericidal Concentration were evaluated. The synthesized Ta- MOF, in addition to high catalytic properties, showed high antimicrobial activity with MIC value between 16 and −256 μg/ml, and can be introduced as an agent against bacteria and fungi.
UV-irradiating synthesis of cyclodextrin–silver nanocluster decorated TiO2 nanoparticles for photocatalytic enhanced anticancer effect on HeLa cancer cells
Authors: Wang Hongying , Xing Ze , Sun Yan , Jing Yingjie , Zhang Jian , Li Xinyao , Zhang Hailiang , Shakoor Adnan , Guo Junsheng 
Published: September 2022

Titanium dioxide (TiO2) has emerged as a viable choice for several biological and environmental applications because of its high efficiency, cheap cost, and high photostability. In pursuit of this purpose, the research of its many forms has been influenced by these unique aspects. The development of novel TiO2-based hybrid materials with enhanced photocatalytically induced anticancer activity has gained tremendous attention. Here, we have developed a novel photocatalytic material (TiO2–Ag NPs@-CD) by decorating ultrasmall silver nanoparticles (Ag NPs) with per-6-thio-β-cyclodextrin (SH-β-CD) on TiO2 NPs. TiO2–Ag NPs@-CD were characterized by employing various characterization techniques and evaluated for their anticancer activity against HeLa cancer cells using an MTT assay. The biocompatibility of the designed nanoparticles was determined on two normal cell lines, namely, 3T3 and human mesenchymal stem cells (hMSCs). The results show that the TiO2–Ag NPs@-CD induced superior cytotoxic effects on HeLa cancer cells at a concentration of 64 μg/ml. Live-dead staining and oxidative stress investigations demonstrated that cell membrane disintegration and ROS-induced oxidative stress generated by TiO2-Ag NPs@-CD inside HeLa cancer cells are the contributing factors to their exceptional anti-cancer performance. Moreover, TiO2-Ag NPs@-CD exhibited good biocompatibility with 3T3 and hMSCs. These results indicated that the combination of all three components—a silver core, SH-β-CD ligands, and TiO2 nanoparticles—produced a synergistic anticancer effect. Hence, the TiO2-Ag NPs@-CD is a promising material that can be employed for different biological applications.
Shifting microbial communities perform anaerobic oxidation of methane and methanogenesis in sediments from the Shenhu area of northern south China sea during long-term incubations
Authors: Kong Yuan , Lei Huaiyan , Cheng Weidong , Wang Bin , Pan Fulong , Huang Fanfan 
Published: September 2022

Microbial communities and their associated metabolic activities, methanogenesis and anaerobic oxidation of methane (AOM), are the key components of carbon biocycles in continental margin sediments. The composition and diversity of microbial communities in a methanic environment have been widely investigated, but identifying direct correlations between microbial communities and their activities remains a challenge. Here, we investigated shifting microbial communities that performed methanogenesis and AOM in long-term incubations (the longest is up to 199 days). AOM, methanogenesis, sulfate reduction and iron reduction occurred during the incubation, and 16S rRNA gene sequencing showed that some bacteria were maintained or even enriched during the incubation compared to the environmental samples. In contrast, archaeal diversity was reduced, and only some uncultured archaea belonging to the phylum Bathyarchaeota were enriched after treatment with a high sulfate concentration (29.38 mM), suggesting that sulfate might promote their enrichment. Well-known anaerobic methanotrophic archaea (ANME) were not detected, and SEEP-SRB1, which is in syntrophy with ANME, decreased to approximately zero after the incubation. The abundance of known methanogens, such as genera Methanococcoides and Methanosarcina, increased slightly in some incubations but was still present at a low relative abundance (&lt;0.15%). Incubation with a lower sulfate concentration (4 mM) and higher iron content resulted in greater δ13CO2 accumulation, indicating that iron may be the additional electron acceptor for AOM. Based on these results, other unknown or unconventional phylotypes or pathways of methanogenesis and AOM may occur during the incubation. Thus, the diversity of methanogens and anaerobic methanotrophs warrants further investigation.
The prevalence of MAFLD and its association with atrial fibrillation in a nationwide health check-up population in China
Authors: Lei Fang , Qin Juan-Juan , Song Xiaohui , Liu Ye-Mao , Chen Ming-Ming , Sun Tao , Huang Xuewei , Deng Ke-Qiong , Zuo Xiuran , Yao Dongai , Xu Li-Juan , Lu Huiming , Wang Gang , Liu Feng , Chen Lidong , Luo Jie , Xia Jiahong , Wang Lin , Yang QiongYu , Zhang Peng , Ji Yan-Xiao , Zhang Xiao-Jing , She Zhi-Gang , Zeng Qiang , Li Hongliang , Cai Jingjing 
Published: September 2022

Background and aimsThe epidemiological characteristics of MAFLD and its relationship with atrial fibrillation (AF) are limited in China. Therefore, we explored the epidemiological characteristics of MAFLD from adults along with the association of MAFLD and 12-ECG diagnosed AF in a nationwide population from health check-up centers.MethodsThis observational study used cross-sectional and longitudinal studies with 2,083,984 subjects from 2009 to 2017. Age-, sex-, and regional-standardized prevalence of MAFLD was estimated. Latent class analysis (LCA) was used to identify subclusters of MAFLD. Multivariable logistic regression and mixed-effects Cox regression models were used to analyze the relationship between MAFLD and AF.ResultsThe prevalence of MAFLD increased from 22.75% to 35.58% during the study period, with higher rates in males and populations with high BMI or resided in northern regions. The MAFLD population was clustered into three classes with different metabolic features by LCA. Notably, a high proportion of MAFLD patients in all clusters had overweight and prediabetes or diabetes. The MAFLD was significantly associated with a higher risk of AF in the cross-sectional study and in the longitudinal study. In addition, the coexistence of prediabetes or diabetes had the largest impact on subsequent AF.ConclusionOur findings suggested a high prevalence of MAFLD and a high prevalence of other metabolic diseases in the MAFLD population, particularly overweight and glucose dysregulation. Moreover, MAFLD was associated with a significantly higher risk for existing and subsequent subclinical AF in the Chinese population.
Pediatric percentiles for transient elastography measurements - effects of age, sex, weight status and pubertal stage
Authors: Brunnert Lina , Puasa Ika Damayanti , Garten Antje , Penke Melanie , Gaul Susanne , Grafe Nico , Karlas Thomas , Kiess Wieland , Flemming Gunter , Vogel Mandy 
Published: September 2022

Background and aimsTransient Elastography is a non-invasive, cost-efficient, non-ionizing, observer-independent and reliable method to detect liver fibrosis using Liver Stiffness Measurement (LSM) and the degree of fat accumulation in the liver using Controlled Attenuation Parameter (CAP). This study aims to derive reference values for both measures from healthy children and adolescents. Further, we aim to assess the potential influence of age, sex, puberty, and BMI-SDS on CAP and LSM.MethodsWithin the LIFE Child study, amongst others, anthropometric data and pubertal status were assessed. Transient Elastography (TE) was performed using the FibroScan® device in a population-based cohort at 982 study visits of 482 healthy children aged between 10 and 18 years. Percentiles for LSM and CAP were estimated, and the effects of age, sex, puberty and weight status were assessed through hierarchical regression models.ResultsThere was a strong age dependency for LSM with higher values for older children, most pronounced in the upper percentiles in boys. Contrarily, CAP was relatively stable across the age span without considerable difference between boys and girls. We found a significant positive correlation between BMI-SDS and both CAP and LSM for BMI-SDS &gt;1.28. For BMI-SDS &lt; 1.28, the association was also positive but reached statistical significance only for CAP. Further, the association between BMI-SDS and CAP was significantly stronger in younger than in older children. There was no association between pubertal status and CAP. For LSM, we found that children with a high BMI-SDS but not children with normal weight had significantly higher LSM values in Tanner stage 4.ConclusionsAge, sex, pubertal status and weight status should be considered when interpreting LSM and CAP in pediatric patients to facilitate and improve early detection of abnormal liver function, which is associated with common pathologies, such as NAFLD.