bluuebunny/crossref_metadata_2025_split huggingface dataset:
Death In Children's Literature Against The Background Of Selected Child And Childhood Discourses
Authors: Kwaśniewska-Paszta Sandra Marta 
Published: March 2022

The author of the article discusses the sensitive topic of the social taboo of death with regard to the process of the child’s education and development, with particular focus on the use of thanatological literature as a factor shaping the child’s personality. The text presents the perception of the death phenomenon by children, and the essence as well as functions of thanatological literature. It also shows the impact of child and childhood discourses on four well-known literary works for children (at the kindergarten and pre-school age), dealing with the discussed topic. The selected research method consists in qualitative analysis of the content. The works have been selected due to their popularity and presence in scientific publications on the topic of death in children’s literature. Analytical categories of the selected literary works include subjective and objective approach to the child and childhood. The first approach is consistent with the perspective of sociology of childhood and the post-colonial theory, as presented in the article, whereas the second approach refers to the mainstream developmental psychology and the colonial theory.
Based on the analysis of content values of the four literary works, it can be concluded that respect for the child’s subjectivity and the value of their voice supports development of the youngest, among others, in the aspect of dealing with the sensitive topic of death.
The phosphoinositide PI(3,5)P2 inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach
Authors: Gradogna Antonella , Pardo José M. , Carpaneto Armando 
Published: March 2022

Abstract
In the present work, we discuss the way in which the parallel application of the patch-clamp technique and the 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) fluorescence detection for recording luminal proton changes allows the functional characterization of nonelectrogenic potassium/proton vacuolar antiporters of the NHX (Na+/H+ exchanger) family. Moreover, we review the functional role of the tonoplast-specific phosphoinositide PI(3,5)P2, able to simultaneously inhibit the activity of NHXs and CLC-a transporters, whose coordinated action can play an important role in the water balance of plant cells.
The radionuclide monitoring system of the Comprehensive Nuclear-Test-Ban Treaty Organisation : from sample to product
Authors: Matthews M. , Schulze J. 
Published: March 2022

Abstract
The radionuclide monitoring network is one of the four technical components of the compliance-verification system associated with the Comprehensive Nuclear-Test-Ban Treaty (CTBT). Concentrations of radionuclides in the air are monitored at 80 stations worldwide from which spectral and counting data, together with meteorological and state-of-health data, are transmitted through a Global Communication Infrastructure to the Provisional Technical Secretariat in Vienna where the data are analyzed and reported as bulletins for States Parties. Support is provided by 16 Radionuclide Laboratories, which conduct sample analyses if necessary.
Radionuclide laboratories supporting the network of radionuclide stations in verification of the Comprehensive Nuclear-Test-Ban Treaty
Authors: Karhu P. , Clawson R. 
Published: March 2022

Abstract
The radionuclide monitoring network enables continuous world-wide observation of airborne radionuclides in the verification regime of the Comprehensive Nuclear Test-Ban-Treaty (CTBT). The radionuclide technology – analysis of aerosol samples and xenon at 80 sites hosting radionuclide monitoring stations – contributes to the International Monitoring System (IMS). Sixteen radionuclide laboratories will support the radionuclide monitoring network. Their main function is additional analysis of aerosol samples. Samples categorised as Level 5 that contain multiple anthropogenic radionuclides, at least one of which is a fission or an activation product, will always be sent out for laboratory analysis. A number of samples will be re-analysed at the radionuclide laboratories irrespective of category. This serves the purpose of quality control for the results produced by the stations.
Collision probability calculation in cluster lattices with square outer boundaries
Authors: Bogado Leite S. Q. 
Published: March 2022

Abstract
The recently developed module PIJM for computing first-flight collision matrices in cluster geometry for the WIMS code, using an improved numerical technique in the in-plane integrations of the Bickley functions which considers each system region individually, has been extended to treat lattice cells with square outer boundaries. Comparisons with the standard square PIJ routine are presented for a test case, showing significant discrepancies among the results.
Regulatory and safety aspects of ageing in nuclear power plants
Authors: Käufer B. 
Published: March 2022

Abstract
The OECD Nuclear Energy Agency (NEA) is a semi-autonomous body within the OECD established in 1958 with the mandate to promote co-operation among the governments of its participating countries in furthering the development of nuclear power as a safe, environmentally acceptable and economic energy source. While all of groups have detailed programmes involving important aspects, this paper will focus specifically on the work of Committee on Nuclear Regulatory Activities (CNRA) and the Committee on the Safety of Nuclear Installations (CSNI).
Current status of countermeasures for ageing of nuclear power plants in Japan
Authors: Koyama M. , Ishikawa M. , Tajima K. 
Published: March 2022

Abstract
This paper summarizes ageing countermeasure program of the nuclear power plants performed by the Japanese Government and industries and related activities, and describes current research program and utilization of the research results for the aged nuclear power plants. Regulatory bodies (NISA of METI: Nuclear and Industrial Safety Agency of the Ministry of Economy, Trade and Industry') reviewed the ageing issues of nuclear power plants to enhance countermeasures for the aged plants. Nuclear Power Plant Life Engineering Center (PLEC) entrusted by NISA is carrying out the task relating to the aged plants.
miR-223-3p alleviates TGF-β-induced epithelial-mesenchymal transition and extracellular matrix deposition by targeting SP3 in endometrial epithelial cells
Authors: Chen Yanling , Sun Dongyan , Shang Di , Jiang Zhihe , Miao Pan , Gao Jian 
Published: March 2022

Abstract
Intrauterine adhesion (IUA) is the clinical manifestation of endometrial fibrosis. The dysregulation of microRNAs (miRNAs) has been confirmed to implicate in a diversity of human diseases, including IUA. Nevertheless, the specific function of miR-223-3p in IUA remains to be clarified. Reverse transcription quantitative polymerase chain reaction analysis displayed the downregulation of miR-223-3p in IUA tissues and endometrial epithelial cells (EECs). Results from wound healing assay, Transwell assay and western blotting showed that TGF-β facilitated the migration and invasion of EECs and induced epithelial-mesenchymal transition (EMT) process as well as extracellular matrix (ECM) deposition. Overexpression of miR-223-3p in EECs was shown to suppress the effects induced by TGF-β. Bioinformatics analysis and luciferase reporter assay revealed the binding relation between miR-223-3p and SP3. SP3 was highly expressed in IUA and its expression was inversely correlated with miR-223-3p expression in IUA tissue samples. Additionally, upregulation of SP3 reversed the influence of miR-223-3p on the phenotypes of EECs. In conclusion, miR-223-3p alleviates TGF-β-induced cell migration, invasion, EMT process and ECM deposition in EECs by targeting SP3.
Vitamin D receptor hypermethylation as a biomarker for pediatric adrenocortical tumors
Authors: Bueno Ana Carolina , Stecchini Mônica F , Marrero-Gutiérrez Junier , More Candy Bellido , Leal Leticia Ferro , Gomes Débora Cristiane , de Lima Neto Daniel Ferreira , Brandalise Silvia Regina , Cardinalli Izilda Aparecida , Yunes José Andres , Junqueira Thais , Scrideli Carlos Alberto , Molina Carlos Augusto Fernandes , Ramalho Fernando Silva , Tucci Silvio , Coeli-Lacchini Fernanda Borchers , Moreira Ayrton Custodio , Ramalho Leandra , Vêncio Ricardo Zorzetto Nicoliello , De Castro Margaret , Antonini Sonir Roberto R 
Published: March 2022

Objective
Pediatric adrenocortical tumors (pACT) display complex genomic backgrounds, lacking robust prognostic markers and targeted therapeutic options. Vitamin D3 receptor (VDR) promoter hypermethylation and underexpression were reported in adrenocortical carcinomas from adult patients. In this study, we aimed to investigate VDR expression levels and methylation status in pACT and their clinical and prognostic significance.

Design
Retrospective cross-sectional study enrolling pediatric patients with ACT from two tertiary referral institutions.

Methods
We evaluated clinicopathological features, VDR mRNA (qPCR) and protein (immunohistochemistry) expression, and VDR-wide methylation of ACT samples from 108 pediatric patients. Fourteen pediatric and 32 fetal and postnatal normal adrenals were used as controls.

Results
Unlike in pre- and post-natal normal adrenals, most pACT lacked nuclear VDR expression and had reduced mRNA levels, especially the carcinomas. Unsupervised analysis of VDR methylation data revealed two groups of pACT with distinct disease features and outcomes. Tumors with high VDR methylation presented lower mRNA levels, and the respective patients presented advanced disease and reduced disease-free and overall survival.

Conclusions
VDR has a role in normal adrenocortical development and homeostasis, which is impaired during tumorigenesis. VDR hypermethylation and underexpression may be both predictive and prognostic biomarkers for pACT.
COVID-19 in Hospitalised Children and Adolescents: Review of the First Pandemic Year at Vilnius University Hospital Santaros Klinikos
Authors: Ivaškevičienė Inga , Donielaitė – Anisė Kamilė , Žilinskaitė Virginija , Vaičiūnienė Daiva , Ivaškevičius Rimvydas 
Published: March 2022

Background. Since the start of the pandemic with SARS-CoV-2 virus, very little data was known about clinical features and outcomes of COVID-19 in children and adolescents not only in Lithuania, but also in other European countries. This study was started in collaboration with 82 participating healthcare institutions across 25 European countries, using a well-established research network—the Paediatric Tuberculosis Network European Trials Group (ptbnet). This multinational, multicentre cohort study was performed during the first wave of the pandemic, between April 1 and April 24, 2020. Each participating country was allowed to continue further research individually encompassing brighter time limits and using the same methodology. We present here data of children hospitalised at Vilnius University Hospital Santaros Klinikos (VUH SK) during the first year of the pandemic.Materials and methods. We included all paediatric patients with PCR confirmed SARS-CoV-2 infection who were hospitalised at VUH SK. The study was performed between March 12, 2020 and March 12, 2021. A standardised data collection spreadsheet was used to record epidemiological, clinical and treatment data.Results. A total of 104 patients were included in the study. The median age of participants was 5 years (IQR 1.0-11.0, range 0-17 years). Males accounted for 50 (48%) of all patients. The average duration of hospitalisation was 3 days. Ten (9.6%) patients had pre-existing medical conditions. Among all hospitalised patients 16 (15%) were asymptomatic, 5 (4.8%) were treated in intensive care unit (ICU). The most common symptoms among COVID-19 patients were pyrexia 71 (68%) followed by upper respiratory tract infection 49 (47%) and gastrointestinal symptoms 33 (32%). Among the entire cohort only 3 (3%) patients required oxygen support, but none of them was started on continuous positive airway pressure (CPAP), mechanical ventilation or extracorporeal membrane oxygenation (ECMO). None of the patients admitted to ICU needed inotropic support. There was no fatal outcome.Conclusions. Our data indicate that COVID-19 may affect children of any age. The COVID-19 disease was usually mild in hospitalized children and adolescents. The most common clinical findings of COVID-19 were pyrexia and symptoms of upper respiratory tract infection. Severe COVID-19 disease cases when oxygen support or treatment in ICU was required were very rare. No patient received antiviral drugs for Covid-19 treatment. There was no fatal outcome due to COVID-19 in our study population.
Some Reflexions on Jan Čep’s Short Stories in Exile
Authors: Bidaud Samuel 
Published: March 2022

This article focuses on the six short stories that the Czech writer Jan Čep wrote during his exile in France, when he fled the new Czechoslovak communist regime after 1948. These texts are as follows: Les Tziganes [The Gypsies], which was written in French and published in the journal Terre humaine in 1952, Tajemství Kláry Bendové [The Secret of Klára Benda], Před zavřenými dveřmi [In Front of the Closed Door], Ostrov Ré [Ré Island], Květnové dni [Days of May], and Tři pocestní [Three Pilgrims]. These short stories are interesting for several reasons. First of all, they present a changing point in Čep’s themes, for they take place during the more recent events – the Second World War and the arrival to power of the communist regime, – and evoke a humanity who has been destroyed. The existential drama of the individual, which had previously been predominant in Čep’s work, is now extended to the entire human community. Fear has separated people from each other, and every genuine personal relation has become impossible. What’s more, language is incapable of bringing together human beings. True communication implies a form of silence, in which God can speak to the heart of man, and where the possibility of communion with the others still exists. At the same time, Čep’s characters have to face this recent history, which obliges them to make a choice, in particular as far as exile is concerned. As a consequence, a new form of anguish appears, and is superimposed on Čep’s original one. It gives rise to a feeling of guilt over those who stayed in Czechoslovakia, especially his mother, but also confronts Čep with the inability to write, since he doesn’t manage to find a publisher in France and can no longer write in Czech. As a “pilgrim on the earth”, he endures his suffering and the suffering of the world united to Christ, in communion with Him. Eventually, these later short stories represent a transition for Čep, and reveal that he accepts, as painful as this decision can be, to abandon fiction and to engage in the genre of essay, above all for Radio Free Europe. He will thus manage to give hope to people who suffer behind the Iron Curtain in Czechoslovakia, while expressing his intimate concerns in a very personal way.
¡El tamaño sí importa! Incidencia del tamaño del cebo en la captura de escarabajos coprófagos en el bosque seco tropical
Authors: Martínez-Hernández Neis José , Rangel-Acosta Jorge Luis , Beltrán-Díaz Hernando Javier , Daza-Guerra Carlos Andrés 
Published: March 2022

Introducción: Tradicionalmente, los estudios de escarabajos coprófagos en los bosques secos tropicales (BST) del Caribe colombiano han aplicado metodologías diseñadas para zonas húmedas y andinas del país, lo cual podría estar incidiendo en el rendimiento y la eficiencia del muestreo. Objetivo: Con el fin de aportar a esta discusión, se analizó cómo la cantidad de cebo y el tiempo de operación de la trampa de caída inciden en la efectividad de captura de escarabajos coprófagos en un fragmento de BST en La Reserva Campesina la Flecha, San Jacinto, Colombia. Métodos: Para la captura de los escarabajos, se utilizó trampas de caída, cebadas con tres cantidades diferentes de atrayente: pequeño (34.6 g), mediano (53.8 g) y grande (114.9 g), las cuales permanecieron activas en campo durante 48 h. Cuatro muestreos fueron realizados entre marzo y septiembre de 2015, abarcando por igual la época seca y de lluvia. Resultados: Se registró un total de 4 563 individuos, agrupados en 10 géneros y 27 especies de escarabajos coprófagos. Los mayores valores de riqueza, abundancia y biomasa se presentaron en el cebo grande, a las 48 h, durante la época de lluvias. Sin embargo, el tiempo de operación de la trampa no tuvo efecto en la estimación de la riqueza, abundancia y biomasa de escarabajos independientemente de los tamaños de cebo. Los tres órdenes de la diversidad (0D, 1D y 2D), presentaron valores similares entre las cantidades de cebo durante la época de lluvia, pero en la época seca, los cebos de mayor tamaño presentaron los valores de diversidad más altos. Por su parte con el cebo de mayor tamaño se capturó significativamente más riqueza, abundancia y biomasa de escarabajos de cuerpo pequeño y grande siendo esta situación más notaria durante la época seca. Conclusiones: Los resultados de este trabajo evidencian que para el estudio de los ensamblajes de escarabajos en el BST la utilización de un cebo de mayor tamaño contribuye a una mejor estimación de riqueza, abundancia, diversidad y biomasa, sobre todo durante la época seca, cuando las condiciones ambientales propician que los cebos pierdan su atractividad con mayor rapidez.
Bioactividad e identificación de los ácidos grasos de la esponja marina Tetilla rodriguesi (Tetractinellida: Tetillidae) en el Caribe colombiano
Authors: Quiroz-Lobo Yeinner , Santafé-Patiño Gilmar , Quirós-Rodríguez Jorge A. 
Published: March 2022

Introducción: Durante millones de años, los organismos marinos han venido desarrollando estrategias para adaptarse a los cambios ambientales y con esto, sintetizando una gran variedad de metabolitos secundarios con actividades biológicas. Objetivo: Evaluar las actividades antimicrobiana y antioxidante e identificar los ácidos grasos del extracto metanólico de la esponja marina Tetilla rodriguesi recolectada en la bahía de Cispatá. Métodos: Los especímenes de T. rodriguesi se sometieron a extracción para obtener el extracto metanólico, del cual se obtuvieron los ácidos grasos que fueron esterificados e identificados haciendo uso de técnicas cromatográficas. Con el extracto metanólico se realizó la actividad antioxidante frente a los radicales ABTS+• y DPPH• y la actividad antimicrobiana por el método de microdilución frente a cepas de referencia de Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis y Candida albicans; además de aislados clínicos de Candida albicans (obtenida en sangre) y Candida krusei (obtenida en catéter). Resultados: Los resultados arrojaron la identificación de 19 ácidos grasos de diferente naturaleza. En el análisis de la actividad antioxidante se pudo encontrar, que la inhibición de los radicales evaluados fue moderada (296.98 ppm para ABTS+• y 3 523.62 ppm para DPPH•). La evaluación de la actividad antimicrobiana mostró, que el extracto metanólico de T. rodriguesi logró reducir en gran medida el crecimiento de todos los microorganismos evaluados. Conclusión: A pesar de que los poríferos tienden a poseer ácidos grasos de gran tamaño, en este trabajó no se encontró la presencia de ácidos grasos de cadena carbonada mayor a 20 miembros. Los resultados de la actividad antioxidante, se asemejan en gran medida al encontrado en otras especies del mismo phylum. En esta investigación, se pudo establecer que el extracto metanólico de T. rodriguesi logró disminuir en gran medida el crecimiento de todas las cepas bacterianas y fúngicas utilizadas.
Immunogenetic profile of MIC (A, B) HLA loci linked to MHC antigenic complex in Russians of the Chelyabinsk Region
Authors: Burmistrova A. L. , Vavilov M. N. , Stashkevich D. S. , Suslova T. A. 
Published: March 2022

The MIC genes are located on chromosome 6 in the class I major histocompatibility complex (MHC) region and encode a membrane-bound stress-inducible protein that acts as a ligand to stimulate the NKG2D activating receptor expressed on the surface of the most natural killer cells (NK). Currently, 7 MIC loci are known, of which only MICA and MICB encode proteins and show a significant allelic polymorphism. The MIC gene polymorphism and their location in the HLA region suggests presence of some ethnic and populational differences for the gene frequencies, linkage disequilibrium of distinct loci, and distribution of HLA-MIC haplotypes, thus making it possible to get information on genetic relationship of human populations. The aim of our study was to assess immunogenetic profile of Russian population in Chelyabinsk Region based on the non-classical HLA loci, i.e., MICA and MICB, in the context of worldwide population data. Methods of the study included immunogenetic typing of 100 donors identifying themselves as Russians, taken from the Registry of Stem Cell Donors at the Chelyabinsk Regional Blood Transfusion Station. The 2 loci (MICA and MICB) were typed at basic resolution, using PCR technique with sequence-specific primers (SSP-PCR). Gene frequencies (GF) were calculated using programs for immunogenetic research (Arlequin 3.5).Among Russian population from Chelyabinsk Region, the following characteristics of the MICA gene distributions were found: MICA *008, *002, *010, *009, frequency of > 7%; average frequencies, for MICA *004, *007, *018, *017; whereas MICA *027, *011, *006, *009:02, *049, *012, *016 was registered at a frequency of < 3.5%. MICB gene profile was as follows: MICB *005:02, *004, *002, *008 at a frequency of > 6%; at a frequency of 4% MICB *003, *005:03; MICB *005:01, * 005:04, * 009N, MICB *013, *014 at a frequency of0.5%. As based on calculated genetic distances (according to Ney) for the MICA locus, the dendrogram and scatter plot were designed by means of multidimensional scaling (MDS) method, presenting location of 30 world populations, including data on Russians in Chelyabinsk Region. The smallest genetic distances between the population of Russians from Chelyabinsk Region and other world populations were found between the population of Slovenia, as well as the USA population of European origin. As based on scatterplot obtained by the MDS approach for MICA gene frequencies, using the data of cluster analysis, we have found that the population of Russians from Chelyabinsk Region belongs to a cluster of typical European populations.The obtained patterns could be used for practical purposes to create a registry of stem cell donors in Russia. In addition, the data may be used as a control group for further research in the area of HLA-disease association, and could be also demanded by the specialists in population ethnogenesis.