bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tropomyosin micelles are the major white components in the boiled soup of shellfish
Authors: Akihiro Takashi , Yasui Ryou , Yasuhira Shinji , Matsumoto Ken-ich , Tanaka Yasuhiro , Matsuo Yasuhiro , Shimazu Hidehisa , Matsuzaki Takashi , Matsumoto Shingo , Ishida Hideki 
Published: January 2022

Abstract
Basket clam soup, a popular Asian dish, is prepared by boiling clams in hot water. The soup is generally cloudy and considered more delicious as cloudiness increases. However, the identity of the whitening ingredients and their relationship with taste remain unclear. In this study, we aimed to identify the components that contribute to the white color of the boiled soup. The white component was precipitated with trichloroacetic acid and reacted positively with ninhydrin, indicating the presence of proteins. The proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and an intense band was observed at 33 kDa. Peptide mass fingerprinting of this band using matrix-assisted laser desorption/ionisation-time-of-flight tandem mass spectrometry revealed the protein to be tropomyosin. Basket clam tropomyosin expressed and purified from Escherichia coli turned the extracted solution white, confirming that tropomyosin contributed to the white color of clam soup.
First Manifestation of AQP4-IgG-Positive Neuromyelitis Optica Spectrum Disorder Associated with the COVID-19 mRNA Vaccine BNT162b2
Authors: Umezawa Shu , Ioka Katsura , Aizawa Satoshi , Tashiro Yuichi , Yoshizawa Kazuo 
Published: January 2022

Abstract
BNT162b2 is one of the effective COVID-19 vaccines. However, some researchers have also reported that the vaccines caused some neurological complications. Here, we present a case of a 52-year-old female who developed aquaporin (AQP) 4-IgG-positive neuromyelitis optica spectrum disorder (NMOSD) fourteen days after the first dose of BNT162b2. She experienced pain of the neck, weakness of the left arm and leg, numbness of the left hand, and impaired temperature sensation of the right leg. MRI showed T2WI hyperintense lesions in the area postrema and cervical spinal cord ranging from C1 to C6 level, and Gd-enhanced lesions from C3 to C5 level; especially left lateral column was predominantly enhanced. Cell-based assays showed anti-AQP4 antibody (AQP4Ab) was positive. We diagnosed AQP4-IgG-positive NMOSD. After high-dose glucocorticoid therapy, she is showing improved symptoms. The present case was characterized by the findings that a Gd-enhanced lesion in the cervical cord localized dominantly at the left lateral column, consistent with the side of the shoulder where the vaccine was injected. Many studies suggested that AQP4-IgG-positive NMOSD development has multistep mechanisms following the blood-brain barrier (BBB) breakdown. We suspected that BNT162b2-associated immune responses lead to BBB disruptions. Through the limitedly damaged BBB, the plasma cells producing AQP4Abs might be recruited to CNS, and AQP4Abs might bind to the cervical cord and the area postrema. A large population-based study revealed that BNT162b2-associated complications were less likely to be observed than COVID-19 infectious symptoms. However, considering the present case, neurologists need to observe the conditions following vaccination.
Determination of Sex Ratio In Bovine Semen Using SYBR Green Real-Time PCR
Authors: Harshini Vemula , Karthickeyan S.M.K. , Kumarasamy K.G P. , Tirumurugaan  , Jeevan C. 
Published: January 2022

Abstract
A SYBR green real-time PCR assay was developed to find out the sex skewness in bovine sex-sorted semen samples. The qPCR assay of PLP and SRY genes revealed the mean values of X- and Y-bearing spermatozoa as 50.24 ± 0.65 and 49.75 ± 0.62 per cent in unsorted, and 91.80 ± 0.79 and 8.20 ± 0.73 per cent in X-enriched semen samples respectively.. The amplification efficiencies of the PLP and SRY primers were 99.25 and 98.03 per cent respectively. The method was validated by a series of repeatability and reproducibility assays which revealed low co-efficients of variations as 2.19 and 3.12 per cent respectively Thus becoming a reliable and inexpensive tool to evaluate the sorted semen on routine basis and validation of other sperm sexing technologies.
SARS-CoV-2 Delta AY.1 variant Cluster in an accommodation facility for COVID-19
Authors: Ohishi Takayuki , Yamagishi Takuya , Kurosu Hitomi , Kato Hideaki , Takayama Yoko , Anan Hideaki , Kunishima Hiroyuki 
Published: January 2022

Abstract
This is a case report on a cluster infection of novel severe acute respiratory syndrome coronavirus 2 delta AY.1 variant at an accommodation facility and the subsequent attempts to isolate individuals who tested positive. The background that facilitated this cluster was investigated, and the conditions in which infection was established, the infection route, and the effectiveness of routine measures were evaluated. Ninety-nine staff members had been working at the accommodation facility at the time of infection, and it was estimated that 10 members were infected with the delta AY.1 variant. Our results suggest that infection of staff from a patient staying overnight should be excluded. The factors contributing to the cluster infection involved short-distance conversations with individuals wearing non-woven three-layer masks moved out of position (non-woven) and gathering together with individuals wearing non-woven masks in hypoventilated conditions. Our findings also indicate that this variant is possibly airborne and can infect individuals in enclosed spaces with poor ventilation, even when either infected or exposed individuals wear non-woven masks. The routine maintenance of systems established for the detection of infections and prompt and appropriate preventive measures following the identification of positive individuals will help prevent further cluster infections.
Uncovering the Potential Mechanism of Xue Fu Zhu Yu Decoction in the Treatment of Intracerebral Hemorrhage
Authors: Xue Dao-jin , Zhen Zheng , Wang Ke-xin , Zhao Jia-lin , Gao Yao , Chen Yu-peng , Shen You-bi , Peng Zi-zhuang , Guan Dao-gang , Huang Tao 
Published: January 2022

Abstract
Background
Chinese herbal medicine (CHM) is characterized by “multi- compounds, multi-targets and multi-pathway”, which has advanced benefits for the preventing and treating complex diseases, but still exists unsolved issues, mainly include unclear material basis and underling mechanism of prescription. Integrated pharmacology is a hot cross research area based on system biology, mathematic and poly-pharmacology. It can systematically and comprehensively investigate the therapeutic reaction of compounds or drugs on pathogenic genes network, and is especially suitable for the study of complex CHM systems. Intracerebral Hemorrhage (ICH) is one of the main causes of death among Chinese residents, which is characterized by high mortality and high disability rate. In recent years, the treatment of ICH by CHM has been deeply researched. Xue Fu Zhu Yu Decoction (XFZYD), one of the commonly used prescriptions in treating ICH at clinic level, has not been clear about its mechanism in treating ICH.
Methods
Here, we established a strategy, which based on compounds-targets, pathogenetic genes, network analysis and node importance calculation. Using this strategy, the core compounds group (CCG) of XFZYD was predicted and validated by in vitro experiments. The molecular mechanism of XFZYD in treating ICH was deduced based on CCG and their targets.
Results
The results show that the CCG with 43 compounds predicted by this model is highly consistent with the corresponding Compound-Target (C-T) network in terms of gene coverage, enriched pathway coverage and accumulated contribution of key nodes at 89.49%, 88.72% and 90.11%, respectively, which confirmed the reliability and accuracy of the effective compound group optimization and mechanism speculation strategy proposed by us.
Conclusions
Our strategy of optimizing the effective compound groups and inferring the mechanism provides a strategic reference for explaining the optimization and inferring the molecular mechanism of prescriptions in treating complex diseases of CHM.
Comparison of Electrophysiological Indices of Children With Attention Deficit Hyperactivity Disorder (ADHD) Comorbid With and Without Reading Disorder (ADHD &amp; RD)
Authors: Chalabianloo Gh.Reza , keshtgar zahra , Noorazar Gh.Reza , Poormohammad Ahmad 
Published: January 2022

Abstract
BackgroundAttention Deficit Hyperactivity Disorder (ADHD) is a neuropsychiatric disorder. Most children and adolescents with ADHD have at least some developmental or mental disorders identified from the early years of elementary school. The most common of these are educational and learning problems in these children, which are probably due to the attention deficits of these children. Therefore, it is expected that the cortical activity pattern of ADHD children is different from ADHD comorbid with learning disabilities, which we have examined in this study.MethodsThis study evaluated the pattern of cortical activity in children 6 to 12 years old with ADHD comorbid with and without the reading disorder (ADHD & RD) using 21-channel electroencephalography. Multivariate analysis of variance with repeated measures in a 2 * 3 * 7 design and T-test was used for statistical analysis.ResultsThe results show that in ADHD children, the activity of different bands increases compared to ADHD comorbid with RD children. In the ADHD group compared to the ADHD comorbid with RD group, the theta/beta ratio in all three regions, especially the anterior region, is higher than the theta/alpha activity in those areas, and this group has significantly higher activity in all three brain regions, especially the anterior region, compared to ADHD comorbid with RD patients.ConclusionsFunctional changes in the left parietal cortex, which is part of the frontoparietal attention network and involved in phonological processing, reading, and calculation, are evident in children with ADHD comorbid with and without the reading disorder (ADHD & RD). However, ADHD without reading disorder shows more activation of the frontoparietal network than ADHD comorbid with reading disorder, and therefore it can be said that ADHD without reading disorder exerts more cognitive control. Therefore, it is likely to be possible to prevent educational problems in these children by using neurofeedback or prescribing drugs that increase the activity of the areas involved in attention.
Development and Optimization of A High-Throughput 3D Rat Purkinje Neuron Culture to Study Paraneoplastic Cerebellar Degeneration
Authors: Uggerud Ida Margrethe , Krakenes Torbjorn , Hirai Hirokazu , Vedeler Christian Alexander , Schubert Manja 
Published: January 2022

Abstract
Improved understanding of the mechanisms involved in neurodegenerative disease has been hampered by the lack of robust cellular models that faithfully replicate in vivo features. Here, we present a refined protocol for generating age-dependent, well-developed and synaptically active rat Purkinje neurons in a 3D cell network culture which are responsive to a disease inducer. Using our model, we found that the application of autoantibody Yo, a paraneoplastic cerebellar degeneration (PCD) inducer, alters the structure of the dendritic arbour of cultured Purkinje neurons. The numbers of dendrites per branch-order, the branch-order in itself and the dendritic length were reduced by anti-Yo, proving a functional role for anti-Yo in the pathogenesis of PCD. Our new ex-vivo model is flexible and can be used to investigate disease mechanisms that disturb Purkinje neuron function and communication in 3D. Since it is possible to use the approach in a multi-well format, this method also has high-throughput screening potential.
Impact of Multi-parametric Analyses on Normal Left Atrial Strain by Cardiovascular Magnetic Resonance Feature Tracking
Authors: Eckstein Jan , Körperich Hermann , Paluszkiewicz Lech , Burchert Wolfgang , Piran Misagh 
Published: January 2022

Abstract
Left-atrial (LA) strain is the result of complex hemodynamics, increasingly assessed by feature-tracking cardiovascular magnetic resonance (CMR). We investigate the value of multi-parametric regression (MPR) analyses and the influence of the heart rate on LA-strain. As LA-strain data remains limited, CMR-quantified sex- and age-dependent normal values were derived. After following a health assessment questionnaire, 183 healthy volunteers (11-70 years, 97 females, median 32.9±28.3 years) were recruited for LA-strain assessment. LA volumetric data, left ventricular strain, transmitral and pulmonary venous blood flow parameters were utilized to create clusters for MPR analyses for all subjects and heart rate-specific subgroups (range: 60–75 beats-per-minute, N=106). In comparison to the total cohort, subgroups showed no gender differences (p>0.05) for LA reservoir, conduit and booster strains (all: 47.3±12.7%; 29.0±15.5%; 17.6±5.4%) and strain rates (all: 2.1±1.0 s−1; -2.9 ± 1.5 s−1; -2.3 ± 1.0 s−1). MPR analyses identified parameter clusters with large effect size (|R²|≥0.26) for reservoir-, conduit- and booster strain and corresponding active and passive cardiac functional parameters. Increased correlations for the subgroup were found. In contrast to previous studies, heart rate selected subgroups showed no gender differences in LA-strain. MPR analyses improve characterization of LA-strain at selected heart rates.
DNA Aptamers Specific for Legionella Pneumophila: Systematic Evolution of Ligands by Exponential Enrichment in Whole Bacterial Cells
Authors: Xiong Lina , Xia Mingchen , Wang Qinglin , Meng Zhen , Zhang Jie , Yu Guohui , Dong Zhangyong , Lu Yongjun , Sun Yunhao 
Published: January 2022

Abstract
Legionella pneumophila is the major causative agent of Legionnaires’ disease and Pontiac fever, which pose major public health problems. Rapid detection of L. pneumophila is important for global control of these diseases. Aptamers, short oligonucleotides that bind to targets with high affinity and specificity, have great potential for use in pathogenic bacterium detection, diagnostics, and therapy. Here, we used a whole-cell SELEX (systematic evolution of ligands by exponential enrichment) method to isolate and characterize single-stranded DNA (ssDNA) aptamers against L. pneumophila. A total of 60 ssDNA sequences were identified after 17 rounds of selection. Other bacterial species (Escherichia coli, Bacillus subtilis, Pseudomonas syringae, Staphylococcus aureus, Legionella quateirensis, and Legionella adelaidensis) were used for counterselection to enhance the specificity of ssDNA aptamers against L. pneumophila. Four ssDNA aptamers showed strong affinity and high selectivity for L. pneumophila, with Kd values in the nanomolar range. Bioinformatic analysis of the most specific aptamers revealed predicted conserved secondary structures that might bind to L. pneumophila cell walls. In addition, the binding of these four fluorescently labeled aptamers to the surface of L. pneumophila was observed directly by fluorescence microscopy. This is the first study to use SELEX to target L. pneumophila whole cells. The aptamers identified in this study could be used in the future to develop medical diagnostic tools and public environmental detection assays for L. pneumophila.
Modeling and Analysis of D–shaped Plasmonic Refractive Index and Temperature Sensor Using Photonic Crystal Fiber
Authors: Tondro Amin Sayyad , sadeghi mojtaba , Kamaly Abbas , Adelpour Zahra , Emamghorashi A. 
Published: January 2022

Abstract
A plasmonic D–shaped photonic crystal fiber (PCF) sensor for refractive index (RI) and temperature is designed and studied in this paper. The RI channel is made up of a silver thin layer deposited on the plane of the D–type structure, and the temperature sensing channel is formed by a silver nanowire and benzene filled in an elliptical hole in the PCF. The designed structure can be used for temperature and RI sensing via coupling between the core–guided modes and the surface plasmon modes around Ag nanowire and Ag thin layer. The coupling characteristic and sensing performance of the proposed dual–functional sensor is studied in the present work. Results show that the maximum sensitivity in terms of RI units (RIU) is 6.9 µm/RIU in the range of 1.33–1.38, and the maximum temperature sensitivity is 3 µm /°C in the range of 27°C–67°C.
Mitophagy Eliminates the Accumulation of SARM1 on the Mitochondria, Alleviating Programmed Axon Death in Acrylamide Neuropathy
Authors: Wang Shuai , Yong Hui , Zhang Cuiqin , Kang Kang , Song Mingxue , Yang Yiyu , Huang Zhengcheng , Wang Shu'e , Ge Haotong , Zhao Xiulan , Song Fuyong 
Published: January 2022

Abstract
Sterile-α and toll/interleukin 1 receptor motif containing protein 1 (SARM1) is the central executioner of programmed axon death (Wallerian degeneration). Although it has been confirmed to have a mitochondrial targeting sequence and can bind to and stabilize PINK1 on mitochondria, the biological significance for mitochondrial localization of SARM1 is still unclear. The relationship between mitochondrial quality control mechanisms and programmed axon death also needs to be clarified. Chronic acrylamide (ACR) intoxication cause typical pathology of axon degeneration involving early axon loss. Here, we demonstrated that the SARM1 dependent Wallerian axon self-destruction pathway was activated following ACR intoxication. Moreover, increased SARM1 was observed on the mitochondria, which interfered with the mitochondrial quality control mechanisms. As a protective response to stress, mitochondrial components enriched in SARM1 were isolated from the mitochondrial network through an increased fission process and were degraded in an autophagy-dependent manner. Importantly, rapamycin (RAPA) administration eliminated mitochondrial accumulated SARM1 and inhibited axon loss. Thus, mitochondrial localization of SARM1 may be complement to the coordinated activity of NMNAT2 and SARM1, and may be part of the self-limiting molecular mechanisms of programmed axon death. In the early latent period, the mitochondrial localization of SARM1 will help it to be isolated by the mitochondrial network and to be degraded through mitophagy to maintain local axon homeostasis. When the mitochondrial quality control mechanisms are broken down, SARM1 will cause irreversible damage for axon death.
SPS-CTDI Dual-evaluation Model-oriented Nursing Undergraduate Gold Course Development and Implementation
Authors: Yan Wang , Nan Wang , Yingting Zhou , Yuanyuan Li 
Published: January 2022

Abstract
Objective: When applying the ”golden lesson” concept to pediatric nursing teaching, under the condition that the two evaluation models support each other, study the application effect of the new teaching concept in the teaching process.Method: Two classes of nursing major of 2018 were used as the golden lesson group and the tradition group. The tradition group used traditional teaching methods, and the golden lesson group used the ”golden class” concept combined with the ”Internet +” platform for teaching. After the course, compare the two groups of students’ problem-solving scores and their theoretical and practical scores.Results: The problem-solving ability of the students in the golden lesson group was better than the tradition group in terms of ”negative problem orientation”, ”avoidance style” and ”impulsive/negligence style” [(8.75±1.32VS12.59±4.7) points, (11.14±2.4) VS13.86±2.36) points, (8.59±2.55VS10.53±2.03)] (P<0.05).Conclusion: This teaching mode conforms to the professional development characteristics of nursing students, helps to improve students’ problem-solving ability and strengthens their thinking, but it also puts forward more requirements on teachers’ teaching and practical experience.
Comparative Analysis of Mesenchymal Stem Cells Cultivated in Serum Free Media
Authors: Lee Joo Youn , Kang Min Hee , Jang Ji Eun , Lee Jeong Eon , Yang Yuyeong , Choi Ji Yong , Kang Hong Seok , Lee Uiil , Choung Ji Woong , Jung Hyeryeon , Yoon Young-Chan , Jung Kyung Hee , Hong Soon–Sun , Yi Eugene C. , Park Sang Gyu 
Published: January 2022

Abstract
Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity.
Development and Validation of a Scale to Measure Religious and Spiritual Support in Sri Lankan Patients with Cancer
Authors: Weeratunga Eranthi , Senadheera Chandanie , Hettiarachchi Manjula , Perera Bilesha 
Published: January 2022

Abstract
Background: Religious and spiritual support (RSS) is considered a vital method of coping among patients with cancer and cancer survivors. Measuring religion and spiritual support received by cancer patients has very little evidence in Sri Lanka compared to the western countries. It would be affected on cancer care management and speedy recovery process of cancer patients; could be benefited to reduce long-term suffering of patients with cancer. This study aimed to develop and validate a tool to measure religion and spiritual support in patients with cancer. Methods: WHO guidelines were incorporated into the cross-cultural adaptation of the newly developed religious and spiritual support scale (RSSS). Internal consistency for the overall RSSS was investigated using Cronbach’s alpha. Intra-class correlation coefficient (ICC) was used to examine the test-retest reliability of the RSSS for the same sample. Validity was checked using convergent and divergent validity. Principal component analysis (PCA) was performed to test factorial/construct validity.Results: The Sinhalese version of RSSS showed a high internal consistency (Cronbach’s alpha-0.883). The scale revealed favourable test-retest reliability (ICC = 0.981). The overall RSSS score correlated negatively and positively with depressive symptoms (r= - 0.338, p<0.05) and quality of life scores; overall (r=0.421, p<0.001), physical (r=0.340, p<0.05), psychological (r=0.279, p=0.08), social (r=0.373, p<0.05) and environmental quality of life (r=0.429, p<0.001) confirming satisfactory divergent and convergent validity of the Sinhalese version of the RSSS. Factor analysis with PCA extracted two factors explaining 74.47% of the variance.Conclusions: The Sinhalese version of RSSS is a reliable and valid scale to assess the religious and spiritual support of patients with cancer in Sri Lanka.