bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the Mechanism of Quercetin for the Treatment of Atrial Fibrillation by GEO Gene Chip Combined with Network Pharmacology and Molecular Docking
Authors: Tan Xin , Xian Wei , Li Xiaorong , Chen Yongfeng , Geng Jiayi , Wang Qiyi , Gao Qin , Tang Bi , Wang Hongju , Kang Pinfang 
Published: January 2022

Abstract
Atrial fibrillation (AF) is a common atrial arrhythmia for which there is no specific therapeutic drug. Quercetin (Que) has been used to treat cardiovascular diseases such as arrhythmias. In this study, we explored the mechanism of action of Que in AF using network pharmacology and molecular docking. The chemical structure of Que was obtained from Pubchem. TCMSP, Swiss Target Prediction, Drugbank, STITCH, Binding DB, Pharmmapper, CTD, GeneCards, DISGENET and TTD were used to obtain drug component targets and AF-related genes, and extract AF and normal tissue by GEO database differentially expressed genes by GEO database. The top targets were IL6, VEGFA, JUN, MMP9 and EGFR, and Que for AF treatment might involve the lipid and atherosclerosis pathway, the role of AGE-RAGE signaling pathway in diabetic complications, MAPK signaling pathway and IL-17 signaling pathway. In addition, molecular docking showed that Que binds strongly to key targets and is differentially expressed in AF. This study systematically elucidated the key targets of Que treatment for AF and the specific mechanisms, providing a new direction for further basic experimental exploration and clinical treatment.
Assessing the growth response of Vitis vinifera L. cv. Xynisteri, Maratheftiko, Shiraz and Sauvignon Blanc to different irrigation regimes.
Authors: Copper Alexander Willem , Koundouras Stefanos , Bastian Susan E. P. , Johnson Trent , Collins Cassandra 
Published: January 2022

Abstract

The world’s changing climate is placing great pressure on the resources for sustainable viticulture. With this, it has become necessary to investigate grape varieties that are well adapted to hot climates. The aims of this study were to (1) assess the response of Xynisteri to different irrigation regimes, and (2) compare the performance of Xynisteri, Maratheftiko, Shiraz and Sauvignon Blanc grown in pots with different irrigation regimes. Trial one was established in a commercial Xynisteri vineyard in Cyprus under three different irrigation regimes - full, 50% and no irrigation in 2019. Trial two compared three irrigation regimes - full, 50% and 25% in a potted trial of Xynisteri and Sauvignon Blanc conducted in Cyprus in 2019. Trial three was a potted trial of Xynisteri, Sauvignon Blanc, Maratheftiko and Shiraz with the same three irrigation regimes conducted in Australia in 2020/21. Vine performance and physiology measurements were taken in both trials. Fruit composition analysis, yield (field trial only), shoot, trunk and root mass measurements were performed at the end of the season. Few differences between measures were found between irrigation regimes in the field trial. Fruit composition analysis revealed fructose to be lowest in the full irrigation group compared to deficit and no irrigation treatments. The potted trial in 2019 demonstrated that for all three irrigation regimes, Xynisteri had higher stem water potential, stomatal conductance and chlorophyll content than Sauvignon Blanc. Xynisteri produced greater end of season root, shoot and leaf mass than Sauvignon Blanc under all irrigation regimes. In 2020/21, Xynisteri had greater end of season root, shoot and leaf mass than Maratheftiko and Sauvignon Blanc with Shiraz the lowest. Few significant differences in stem water potential were observed in the early stages of the trial. However, toward the end of the trial and with reduced irrigation, Xynisteri and Maratheftiko had higher stem water potential than Shiraz and Sauvignon Blanc. Xynisteri had higher stomatal conductance and chlorophyll content than Maratheftiko and both were higher than Sauvignon Blanc and Shiraz. These results indicate that Xynisteri in particular may possess better cultivar specific growth traits than Shiraz and Sauvignon Blanc when grown under the same environmental conditions and in turn may be a more appropriate choice in areas where water is limited.
Wireless Power Transfer System for Mobile Robots via Magnetic Resonant Coupling with Impedance Matching
Authors: HIJIKATA Wataru , Ohori Toshiki , Li Xiang , Nakanishi Hideyuki , Ozawa Shigeki 
Published: January 2022

Abstract
Wireless power transfer via magnetic resonant coupling can be used to supply power to a mobile robot within a few meters of a transmitter coil. However, when the robot moves or its power consumption fluctuates, its input impedance varies and causes power reflection. Therefore, we propose the use of a driver coil on the transmitter side to match the input impedance. The input impedance is matched and power reflection is eliminated by regulating the coupling coefficient between the driver and the transmitter. During experiments, the transmitting efficiency showed good agreement with the calculated value, and the input impedance was matched under varying distances and load resistances. Therefore, the proposed system was demonstrated to solve the power reflection problem in mobile robots.
Melatonin Induces Cell Cycle Arrest, Inhibits Cell Proliferation and Accelerates Apoptosis by Modulation of CDK4 in NSCLC
Authors: Zhou Mengxi , Wang Yueguo , Shen Jilong , Fei Guanghe 
Published: January 2022

Abstract
Purpose
To explore whether melatonin affect the progression of cell cycle and exert anticancer activities via the modulation of CDK4 in NSCLC .
Methods
Cells treated with melatonin were used for assessing the anticancer effect of melatonin. Cells transfected with lentivirus for CDK4 upregulation or downregulation was constructed to evaluate the role of CDK4 in melatonin-induced anticancer effect. The protein and mRNA level of CDK4, PCNA and Bax were detected by western blotting and qRT-PCR. The application of flow cytometry was used for analyzing the distribution of cell cycle and apoptosis. Animal model of subcutaneous tumor was constructed and used for further study in vivo.
Results
We found that melatonin inhibited cell viability, colony formation, downregulated the expression of CDK4 and PCNA while upregulated the level of Bax. Besides, melatonin decreased the phosphorylation of ERK. Importantly, inhibition of ERK activation by PD98059 particapated in melatonin-induced downregulation of CDK4. Furthermore, melatonin led to G1 arrest and cell apoptosis. CDK4 knockdown enhanced melatonin-induced cell cycle arrest while CDK4 overexpression reversed the effect. Additionally, the animal experiment showed that melatonin decreased the level of CDK4 and inhibited tumor growth. However, the anti-tumor effect of melatonin was reversed by CDK4 overexpression.
Conclusion
Taken together, CDK4 involved in anti-cancer activities of melatonin. Melatonin led to G1 arrest, blocked G1-to-S transition, as a result, inhibited cell proliferation and accelerates apoptosis via suppressing CDK4 signaling. Targeting CDK4 inhibition and combining it with melatonin has protential to be a novel strategy for NSCLC.
Point-of-Care Testing in Community Pharmacies: A Mixed-Methods Study on Users and Pharmacists’ Facilitators and Barriers
Authors: Figueira Inês , Teixeira Inês , Rodrigues António Teixeira , Gama Ana , Dias Sónia 
Published: January 2022

Abstract
Background

Point-of-care tests can contribute to earlier diagnosis and treatment of infectious diseases with the potential to prevent chronic stages. As part of the Fast-Track Cities initiative, a pilot was initiated in community pharmacies in Portugal.
Aim

To characterize the individuals using point-of-care screening tests for human immunodeficiency virus, hepatitis C and hepatitis B virus infections in community pharmacies, their behaviours and motivations to perform the tests, as well as understand the facilitators and barriers from the perspectives of pharmacists.
Method

A mixed-methods study was conducted. A survey was applied to test users in pharmacies between May and December 2019, and three focus groups were conducted with pharmacists involved in the initiative. Qualitative data were analysed according to thematic content analysis.
Results

A total of 210 questionnaires were collected. Point-of-care tests users were predominantly male, mean age of 35 years, the majority were foreign-born and had higher education level. Almost half of the users were first time tested and the main reason for screening was unprotected intercourse. Pharmacists identified speed, confidentiality, counselling provided to users, pharmacists’ initial training to perform the tests and trust in the pharmacist as facilitators of these tests. Stigma associated with infections, the procedure, logistical conditions and the referral process were considered as barriers.
Conclusion

Pharmacies are an effective screening site, with particular relevance for individuals who are first tested, heterosexuals and some migrants. Nevertheless, it is necessary to understand and reduce barriers and increase the support of specific groups.
Livelihood Vulnerability of Char Land Communities to Climate Change and Natural Hazards in Bangladesh: An Application of Livelihood Vulnerability Index
Authors: Mamun Abdullah Al , Islam A R M Towfiqul , Alam GM Monirul , Sarker Md. Nazirul Islam , Erdiaw-Kwasie Michael Odei , Bhandari Humnath , Mallick Javed 
Published: January 2022

Abstract
Bangladesh is one of the most vulnerable countries to climate change in the world. In general, Charland (Riverine Island) communities are frequently affected by floods, riverbank erosion, and other climatic hazards, which cause many to lose their sources of livelihoods and properties and making them more vulnerable. Using survey data of 262 rural households, this study investigates the extent of livelihood vulnerability to climate change and natural hazards of the Charland communities by applying the climate change vulnerability index (CVI) (i.e. UN-IPCC vulnerability framework) and the livelihood vulnerability index (LVI) to develop context-specific interventions for building climate and livelihood resilience. The two approaches of vulnerability assessment were modified to incorporate local contexts and indigenous knowledge into 41 sub-components. The result shows that LVI and CVI values are different between Charland communities. The LVI index shows that households in Char Jotindro-Narayan (0.148) are more vulnerable than Char Kulaghat (0.139). The CVI values for Char Jotindro-Narayan (0.633) are slightly lower than for Char Kulaghat (0.639). The major vulnerability factors were identified as the social networks, food self-sufficiency, natural disasters, and climatic variability. The study also indicates that flood, riverbank erosion, unemployment, and access to communication, market, and basic service opportunities are the major biophysical and socioeconomic factors determining livelihood vulnerability. The context-specific sustainable policies and development initiatives are required to improve the adaptive capacity of Charland communities across Bangladesh and thereby building their climate and livelihood resilience.
A novel algorithm to allocate customers and retailers in a closed-loop supply chain under probabilistic demand
Authors: Keramatlou Omid , Javadian Nikbakhsh , Didehkhani Hosein , Amirkhan Mohammad 
Published: January 2022

Abstract
In this paper, a closed-loop supply chain (CLSC) is modeled to obtain the best location of retailers and allocate them to other utilities. The structure of CLSC includes production centers, retailers’ centers, probabilistic customers, collection, and disposal centers. In this research, two strategies are considered to find the best location for retailers by focusing on 1- the type of expected movement 2- expected coverage (distance and time) for minimizing the costs and maximizing the profit by considering the probabilistic customer and uncertainly demand. First of all, the expected distances between customers and retailers are calculated per movement method. These values are compared with the Maximum expected coverage distance of retailers, which is displayed in algorithm 1 heuristically, and the minimum value is picked. Also, to allocate customers to retailers, considering the customer's movement methods and comparing it with Maximum expected coverage time, which is presented in Algorithm 2 heuristically, the minimum value is chosen to this end, a bi-objective nonlinear programming model is proposed. This model concurrently compares Strategies 1 and 2 to select the best competitor. Based on the chosen strategy, the best allocation is determined by employing two heuristic algorithms, and the locations of the best retailers are determined. As the proposed model is NP-hard, a meta-heuristics (non-dominated sorting genetic) algorithm is employed for the solution process. Afterward, the effectiveness of the proposed model is validated and confirmed, and the obtained results are analyzed. For this purpose, a numerical example is given and solved through the optimization software.
Delta infection following vaccination elicits potent neutralizing immunity against the SARS-CoV-2 Omicron
Authors: Ma Mai-Juan , Yao Lin , Gao Hui-Xia , Zhu Ka-Li , Rong Jun , Yang Li , Zhang Zhi , Jiang Ping , Duan Li-Jun , Wang Guo-Lin , Dai Er-Hei 
Published: January 2022

Abstract
Since the initial detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant (B.1.1.529) in November 2021 in South Africa, it has caused a rapid increase in infections globally. The Omicron variant encodes 37 amino acid substitutions in its spike protein, and early reports have provided evidence for extensive immune escape and reduced vaccine effectiveness. We assessed serum neutralizing activity in sera from Delta infection following vaccination of CoronaVac or ZF2001 and Delta infection only against SARS-CoV-2 Wuhan-Hu-1 (WA1), Beta, Delta, and Omicron. We found that sera from Delta infection only could neutralize WA1 and Delta pseudoviruses but nearly completely lost capacity to neutralize Beta and Omicron pseudoviruses. However, Delta infection following vaccination resulted in a significant increase of serum neutralizing activity against WA1, Beta, and Omicron. This study demonstrates that breakthrough infection of Delta in previously vaccinated individuals substantially induced high potency humoral immune response against the Omicron variant and other emerged variants.
PiRNA MW557525 Promotes the Vitality and Pluripotency of Piwil2-iCSCs by Regulating NOP56.
Authors: Jin Liming , Zhang Zhaoxia , Wang Zhang , Tan Xiaojun , Wang Zhaoying , Shen Lianju , Long Chunlan , Wei Guanghui , He Dawei 
Published: January 2022

Abstract
Background: CSCs play an important role in tumor development. Some studies have demonstrated that piRNAs participate in the progression of various cancers. However, the detailed function of piRNAs in CSCs requires further investigation. This study aimed to investigate the significance of some piRNAs in Piwil2-iCSCs. Methods and Results: Differentially expressed piRNAs in Piwil2-iCSCs were screened by high-throughput sequencing. Target genes were predicted by the miRanda algorithm and subjected to GO and KEGG analysis. One of the differential piRNAs, novel piRNA MW557525, was transfected and its target gene NOP56 was silenced in Piwil2-iCSCs, respectively. RT-qPCR, western blot and dual luciferase reporter assay were used to investigate the interaction of piRNA MW557525 and NOP56. We identified the effect of piRNA MW557525 and NOP56 knockdown on cell proliferation, migration, invasion, and apoptosis via CCK-8, transwell assay, and flow cytometry. The expressions of CD24, CD133, KLF4, and SOX2 were detected via WB. The results showed that piRNA MW557525 was negatively correlated with NOP56, and it promoted the proliferation, migration, invasion, and inhibited apoptosis in Piwil2-iCSCs, and it also promoted the expressions of CD24, CD133, KLF4, and SOX2, while NOP56 showed the opposite effect. Conclusions: These findings suggested that novel piRNA MW557525 might be a novel therapeutic target in Piwil2-iCSCs.
Assessing The Performance Characteristics of Handheld Ultrasound In A Rheumatic Heart Disease Screening Program
Authors: Kaltenborn Zachary P. , Zewde Anteneh , Kirsch Jonathan D , Yates Michelle , Tessier Katelyn M. , Nemec Eileen , Johannsen Ronald A 
Published: January 2022

Abstract
Background: Rheumatic heart disease affects 33 million people in low and middle income countries and is the leading cause of cardiovascular death among children and young adults. Penicillin prophylaxis has been shown to improve valvular function among patients with clinically silent or mild disease. Efforts to expand echocardiographic screening are focusing on simplified protocols, non-physician ultrasonographers, and portable ultrasound devices, including handheld ultrasound. Recent advances support the use of single-view screening protocols. With the increasing availability and low cost of handheld devices, prospective studies are needed to evaluate their performance in these settings. Methods: We conducted a cross-sectional pilot study among 19 at-risk school-children participating in a rheumatic heart disease screening program in Ethiopia comparing a handheld ultrasound device (Phillips Lumify) to a fully-equipped portable ultrasound machine (Sonosite M-Turbo). Results: Agreement between devices was similar for expert and non-expert review (84%). However, when reviewed by a non-expert the Lumify identified fewer screen-positive cases (p-value 0.083). We also compared non-expert to expert interpretation by device and found a significant difference in interpretation for the Lumify (p-value 0.025). There was a trend towards shorter jet length by color Doppler in the handheld ultrasound device for both expert and non-expert review. Conclusions: Our study highlights that screening echocardiograms for RHD may yield different results when a handheld ultrasound device is used.
Prevalence and Determinants of Under-Nutrition Among Under-Five Children in Afar Region, Ethiopia: Evidence From 2016 Ethiopian Demographic and Health Survey
Authors: Mengesha Damitie Kebede , Haile Demewez Moges 
Published: January 2022

Abstract
Background: Undernutrition is one of the major public health concerns among infants and young children in the country. Childhood under-nutrition is widespread in low and middle-income countries. This study was initiated to assess the prevalence of under-nutrition and its associated factors among under-five children in Afar Region, Ethiopia. Methods: The data for this study were extracted from the Ethiopian Demographic and Health Survey (EDHS) 2016. The information collected from 937 children born 5 years before the survey was considered in the analysis. A multivariable binary logistic regression analysis was used at a 5% level of significance to determine the individual- and community-level factors associated with childhood malnutrition.Results: The prevalence of stunting, underweight and wasting in Afar region were 41.1%, 36.2% and 17.7%, respectively. About 26.7% of children in Afar region were both stunting and underweight, 12.7% were both underweight and wasting, 7.6% were both stunting and wasting and only 7.0% of children had all the three under-nutrition conditions. Among the factors considered in this study, age of a child in months, anemia level, mothers’ BMI, household wealth index, size of child at birth, number of living children and place of residence were significantly associated with stunting, underweight and wasting in Afar Region. Conclusions: The prevalence of all stunting, underweight and wasting was relatively high. Undernutrition is one of the major public health concerns among children in Afar Region. The effect of these factors should be considered to develop strategies to reduce under-nutrition in the study area. Thus, intervention should be focused on improving the under-nutrition determinants of the children to improve the child's nutritional status and reduce child morbidity and mortality.
A Multivariate Multi-Step LSTM Forecasting Model For Tuberculosis Incidence With Model Explanation In Liaoning Province, China
Authors: Yang Enbin , Zhang Hao , Guo Xinsheng , Zang Zinan , Liu Zhen , Liu Yuanning 
Published: January 2022

Abstract
Background: In addition to COVID-19, tuberculosis (TB) is the respiratory infectious disease with the highest incidence in China. We aim to design a series of forecasting models and find the factors that affect the incidence of TB, thereby improving the accuracy of the incidence prediction. Results: In this paper, we developed a new interpretable prediction system based on the multivariate multi-step Long Short-Term Memory (LSTM) model and SHapley Additive exPlanation (SHAP) method. Moreover, four accuracy measures are introduced into the system: Root Mean Square Error, Mean Absolute Error, Mean Absolute Percentage Error, and symmetric Mean Absolute Percentage Error. Meanwhile, the Autoregressive Integrated Moving Average (ARIMA) model and seasonal ARIMA model are established. The multi-step ARIMA-LSTM model is proposed for the first time to examine the performance of each model in the short, medium, and long term, respectively. Compared with the ARIMA model, each error of the multivariate 2-step LSTM model is reduced by 12.92%, 15.94%, 15.97%, and 14.81% in the short term. The 3-step ARIMA-LSTM model achieved excellent performance, with each error decreased to 15.19%, 33.14%, 36.79%, and 29.76% in the medium and long term. We provide the local and global explanation of the multivariate single-step LSTM model in the field of incidence prediction, pioneering. Conclusions: The multivariate 2-step LSTM model is suitable for short-term forecasts, and the 3-step ARIMA-LSTM model is appropriate for medium and long-term forecasts. In addition, the prediction effect was better than similar TB incidence forecasting models. The SHAP results indicate that the five most crucial features are maximum temperature, average relative humidity, local financial budget, monthly sunshine percentage, and sunshine hours.