bluuebunny/crossref_metadata_2025_split huggingface dataset:
MurF Ligase Inhibitors: An Overview of Antibacterial Activity
Authors: Singh Anjali , Azam Mohammed Afzal 
Published: June 2022

Abstract:
ATP dependent MurC-F ligases are essential for the biosynthesis of peptidoglycan, an essential
bacterial cell wall component that is required for bacterial cell survival. Last, in the series, MurF catalyzes
the ATP-dependent addition of D-Ala-D-Ala dipeptide to UDP-N-acetylmuramyl-tripeptide to form the
UDP-N-acetylmuramy-pentapeptide monomeric precursor of peptidoglycan. Owing to its critical essentiality
in peptidoglycan biosynthesis and absence in eukaryotic counterparts, MurF is considered a promising
target for the design and development of potent antibacterial agents. Several MurF inhibitors have
been designed and evaluated for their MurF inhibitory and antibacterial activity. These include aminoalkylphosphinates,
sulfonamides, diarylquinolones, hydroxylamines, phosphorylated hydroxylamines, thiazolylaminopyrimidines,
2,4,6-trisubstituted 1,3,5-triazines, etc. However, most of the inhibitors developed
till date lack potent antibacterial activity against both Gram-positive and Gram-negative bacteria. In
the present review, an updated status of MurF ligase inhibitors is presented that may provide a useful
source for the design of novel MurF inhibitors with potent and broad-spectrum antibacterial activity.
In vitro and In vivo Evaluation of the Antidiabetic Activity of Solidago virgaurea
Extracts
Authors: Zhu Lin , Zehra Syeda Andleeb , Bhattarai Prapanna , Zhang Jian , Liu Yin , Parveen Zahida , Sajid Muhammad 
Published: June 2022

Background:
Solidago virgaurea (Asteraceae), has been used for more than 700 years for treating cystitis, chronic nephritis, urolithiasis, rheumatism, and inflammatory diseases. However, the antidiabetic activity of Solidago virgaurea has been rarely studied.

Methods:
Three extracts of Solidago virgaurea were prepared and their antidiabetic potentials were evaluated by various cell-free, cell-based, and in vivo studies.

Results:
We found that the Solidago virgaurea contained multiple bioactive phytochemicals based on the GC-MS analysis. The Solidago virgaurea extracts effectively inhibited the functions of the carbohydrate digestive enzyme (α-glucosidase) and protein tyrosine phosphatase 1B (PTP1B), as well as decrease the amount of advanced glycation end products (AGEs). In the L6 myotubes, the Solidago virgaurea methanolic extract remarkably enhanced the glucose uptake via the upregulation of glucose transporter type 4 (GLUT4). The extract also significantly downregulated the expression of PTP1B. In the streptozotocin-nicotinamide induced diabetic mice, the daily intraperitoneal injection of 100 mg/kg Solidago virgaurea methanolic extract for 24 days, substantially lowered the postprandial blood glucose level with no obvious toxicity. The extract’s anti-hyperglycemic effect was comparable to that of the glibenclamide treatment.

Conclusion:
Our findings suggested that the Solidago virgaurea extract might have great potential in the prevention and treatment of diabetes.
The Combination of Chrysin and Cisplatin Induces Apoptosis in HepG2 through
Down-regulation of cFLIP and Activity of Caspase
Authors: Li Xin , Wang Jian-Ning , Luo Yang-Xin , Peng Bao-Ying , Chen Zheng-Hao , Xiong Xi-Kun , Huang Jun-Ming , Chen Mei-Fen , Wang Feng-Yan 
Published: June 2022

Aim:
The study aims to investigate the combined effects of chrysin and cisplatin on hepatoma(HepG2) cell
lines in vivo and in vitro.

Objective:
Studies have suggested that chrysin can enhance the sensitivity of tumor cells to apoptosis. Drug resistance
in tumor cells reduced the effectiveness of chemotherapy drugs such as cisplatin. We investigated whether the combination
of chrysin and cisplatin can induce more apoptosis than chrysin alone and cisplatin alone.

Methods:
HepG2 cells were pretreated with chrysin for 2 h, followed by the addition of cisplatin for another 24 h. The
morphologic changes were observed under inverted microscope and the cell viability was measured using the MTT
test. The protein and cleavage of caspase-3,8,9, PARP, and cFLIP were determined by Western blotting.

Results:
The cell viability of the HepG2 cell can be reduced by the combination of chrysin pretreatment for 2 h and
cisplatin addition for 24 h; Caspase-3,8,9 and PARP were cleaved after 12 h treatment with chrysin and cisplatin; Pancaspase
inhibitor, Z-VAD-fmk, could reverse the apoptosis induced by chrysin and cisplatin in HepG2 cells; cFLIP
was down-regulated by the combination of chrysin and cisplatin, and could be reversed by Z-VAD-fmk; the xenografted
HepG2 cells formed a tumor in one week; At the end of the experiment, there were significant differences in relative
tumor volume (RTV) and relative tumor proliferation rate between the combined group and the control group, the
chrysin group and the cisplatin group; Western blotting showed that the levels of PARP, cFLIP, and caspase-3 proteins
in isolated tumor tissues also decreased under the combined action of chrysin and cisplatin.

Conclusion:
The combination of chrysin and cisplatin induces apoptosis of hepatic tumor in vivo and in vitro. It downregulates
cFLIP and then activates caspase-8, which triggers caspase-mediated apoptosis of HepG2 cell.
Extensive Multifocal Emphysematous Osteomyelitis of Spine: A Rare Case and a Review of Literature
Authors: Angrup Archana , Sharma Bhawna , Lakhanpal Vikas , Goyal Abeer , Shree Ritu , Raj Shashank , Sreenivasan Priya , Biswal Manisha , Ray Pallab 
Published: June 2022

Background:Emphysematous osteomyelitis (EO) is an extremely rare form of osteomyelitis which is complicated mainly by infection with gas-forming organisms. The common causative agents of this disease are mainly members of Enterobacteriaceae family, the most common are Escherichia coli and Klebsiella pneumoniae along with anaerobes. A total of 48 cases of EO have been reported in the literature till now globally and none have documented the isolation of Corynebacterium amycolatum.Case Presentation:We report a rare case of emphysematous osteomyelitis of the spine and pelvis due to Escherichia coli along with the isolation of Corynebacterium amycolatum from the same pus samples on two consecutive occasions in a 50-year-old female with uncontrolled diabetes mellitus, who was successively treated with antibiotics and drainage of pus. We also did a brief review of the literature of all cases reported till now.Conclusion:The role of Corynebacterium amycolatum in the etiology of emphysematous osteomyelitis needs to be evaluated further in future studies as we cannot completely ignore its isolation in two consecutive samples as a mere contaminant.
Knowledge and Perceptions Regarding the use of Antibiotics and Self-diagnosis: A Comparative Study of the Urban and Suburban Communities in Karawang, Indonesia
Authors: Gatera Vesara A. , Supadmi Woro , Prastika Litha M. , Rohaida Deby T. , Barliana Melisa I. , Abdulah Rizky 
Published: June 2022

Background:To avoid antibiotic resistance, acute respiratory infections (ARIs) require the use of appropriate antibiotics. As a result, having the appropriate knowledge and perceptions is critical for achieving successful clinical outcomes.Objective:The purpose of this study was to determine the relationships and differences in knowledge and perceptions about antibiotics and ARI in urban and suburban communities in the Karawang Regency of West Java Province, Indonesia.Methods:We used a validated questionnaire approach to conduct a cross-sectional study in primary health care facilities. We surveyed 440 respondents from urban areas and 464 respondents from suburban areas.Results:Respondents in both urban and suburban areas agreed that self-diagnosis should not be used to prescribe antibiotics. False knowledge and perceptions about antibiotic use were more prevalent in suburban respondents than urban respondents. Additionally, a significant correlation between education levels and knowledge and perception was observed in both areas. Furthermore, significant correlations between age and occupation, as well as knowledge and perceptions, were identified in urban areas.Conclusion:Our study suggests that additional efforts are needed to increase awareness and ensure appropriate antibiotic use in the community.
Biomedical Applications of Agar and its Composites: A Mini-Review
Authors: Surendran Greeshma , Sherje Atul P. 
Published: June 2022

Abstract:
Agar is a flexible biopolymer that forms jelly like consistency when mixed with water. It
is extracted from seaweeds by treatment with alkali and can also be synthesized from fructose-6-
phosphate. Agar and its composites were found to have applications in drug delivery, wound healing,
tumour therapy and hyperthermia treatment. Agar gel formulations have been used for ultrasound
imaging since it has the ability to scatter sound waves. It has been investigated that agar has
the ability to act as a matrix for the modified release of drugs. Agar/collagen composites were found
to be useful in the healing of wounds, burns, and ulcers. Agar also has the ability to act as a matrix
for carbonyl iron, which is found to have good thermal conductivity and became useful for the
treatment of hyperthermia. This review summarizes the properties, sources, biosynthesis, extraction,
and recent biomedical applications of agar and its composites.
The Potential of Microbubbles as a Cancer Eradication Theranostic Agent
Authors: Chaudhary Atul , Setia Aseem , Singh Dilpreet , Bhattacharya Sankha 
Published: June 2022

Abstract:
Microbubbles are a new kind of delivery system that may be used to treat a variety of
illnesses, including cancer. Microbubble is a non-invasive technology that uses microscopic gasfilled
colloidal particle bubbles with a size range of less than 100 micrometres. This unique carrier
has been used in a variety of applications in the last decade, ranging from basic targeting to ultrasound-
mediated drug delivery. The oxygen in the microbubble lasts longer in the water. The drug
release mechanism is highly regulated, since it releases the medication only in the appropriate
areas, increasing the local impact while reducing drug toxicity. This carrier is exceptional in cancer
medication delivery because of its sustained stability, encapsulation efficiency, and drug targeting.
In this paper, we provide a comprehensive analysis of microbubble technology, including its manufacturing
techniques and use in cancer medication delivery.
Optimization of Process for Removal of Fe-Cu from Wastewater
with Biodegradable Adsorbent
Authors: Rane Nitin M. , Shewale Sandeep P. 
Published: June 2022

Background:
Optimization of a process for removing Fe-Cu from wastewater with biodegradable adsorbents.

Objective:
The objective of the study is to remove copper ions from wastewater. The use of low-cost adsorbents was investigated as a replacement to the conventional methods of removing heavy metals from an aqueous solution.

Methods:
Removal of copper (II) from an aqueous solution by the adsorbent made from the jackfruit peels was investigated and analyzed with the help of UV-spectroscopy. The conventional methods of treatment for heavy metal contamination include chemical precipitation, chemical oxidation, ion exchange, reverse osmosis, membrane separation, electrodialysis, and so on. These techniques are very costly, require energy in huge quantities, and generate toxic byproducts. On the other hand, adsorption has been investigated as a cost-effective method for the removal of heavy metals from wastewater. The equilibrium adsorption level was determined as a function of particle size, adsorbent doses, and variable concentration of metal ion solution. Adsorption isotherms of Cu (II) on adsorbents have been decided and correlated with isotherm equations that include Langmuir and Freundlich models.

Results:
From the experiment, the maximum percentage of copper removed is 84.30%, and the maximum adsorption capacity is 11.24 mg/g for particle size of 0.212 mm, 30.98 mg/g for 1 gram of adsorbent dose and 5.23 mg/g for 300 ppm of concentration of metal ion solution.

Conclusion:
The adsorbent made from the jackfruit peels is more effective for removing copper from aqueous solutions emitted from the industrial wastes and can prove to be the best alternative to the conventional method.
OPTIMALISASI KINERJA LEMBAGA AMIL ZAKAT MELALUI STANDAR MUTU ISO 9001
Authors: Majid Nurkholish 
Published: June 2022

Quality management in organizations becomes a necessity to improve performance and quality standards that have been set. Quality standards can be achieved when the organization has obtained ISO 9001 certification. This study aims to examine how the application of ISO 9001 quality standards in zakat and waqf institutions and how organizational performance can be optimized through the study of several journal articles related to ISO 9001 implementation and its role in improving performance in zakat and wakaf institutions which are expected to be amil zakat and wakaf institutions to be more optimal, efficient, quality and standardized. This study uses qualitative research, with primary and secondary data sources that fit this study. The results showed that the implementation of a quality management system in accordance with ISO 9001 standards in zakat institutions was able to produce performance improvements that were measured through the satisfaction of muzakki and all levels of stakeholders as well as financial aspects and sustainable business processes. muzakki is able to encourage improvement in organizational governance that is good corporate governance.Â Manajemen mutu dalam organisasi menjadi kebutuhan guna meningkatkan kinerja dan standar mutu yang telah ditetapkan. Standar mutu dapat dicapai ketika organisasi telah memperoleh sertifikasi ISO 9001. Penelitian ini bertujuan untuk mengkaji bagaimana penerapan standar mutu ISO 9001 dalam lembaga zakat dan wakaf serta bagaimana kinerja organisasi dapat dioptimalkan melalui kajian beberapa artikel jurnal terkait dengan implementasi ISO 9001 dan perannya dalam peningkatan kinerja pada lembaga zakat dan wakaf yang diharapkan lembaga amil zakat dan wakaf menjadi lebih optimal, efisiensi, yang berkualitas dan terstandar. Penelitian ini menggunakan penelitian kualitatif, dengan sumber data primer dan Skunder yang sesuai penelitian ini. Hasil penelitian menunjukkan bahwa penerapan sistem manajemen mutu sesuai dengan standar ISO 9001 dalam lembaga zakat mampu menghasilkan peningkatan kinerja yang diukur melalui kepuasan muzakki dan seluruh jajaran stakeholder serta aspek finansial serta proses bisnis yang berkelanjutan, Lembaga zakat yang memfokuskan pada stakeholder serta peningkatan layanan kepada mustahik dan muzakki mampu mendorong peningkatan tata keola organisasi yang bersifat good corporate governance.
SURVEI TENTANG FAKTOR-FAKTOR PENENTU KEPATUHAN MEMBAYAR ZAKAT DI MASA PANDEMI
Authors: Aligarh Frank 
Published: June 2022

The Covid 19 pandemic has slowed economic growth. The addition of the new poor and the increasing number of unemployed people made the government's burden in providing assistance even heavier. Zakat as an alternative to financial instruments is expected to be an alternative to this problem. However, the potential for zakat must be improved. The main objective of this study is to examine the determinants of zakat paying compliance. This study uses religiosity, trust, income, and gender as predictor variables. This study uses a survey method in data collection. A total of 230 questionnaires were distributed to respondents in Muslim-majority areas (namely Surakarta City), of which 198 were usable. This study uses Structural Equation Modeling to analyze data. The results show that all hypotheses are supported.

Pandemi Covid 19 telah membuat pertumbuhan ekonomi menjadi melambat. Bertambahnya orang miskin baru dan semakin banyaknya pengangguran membuat beban pemerintah dalam memberikan bantuan semakin berat. Zakat sebagai alternatif instrumen keuangan diharapkan menjadi alternatif persoalan tersebut. Walaupun demikian potensi zakat masih harus ditingkatkan. Tujuan utama penelitian ini adalah untuk menguji faktor-faktor penentu kepatuhan membayar zakat. Penelitian ini menggunakan religiusitas, kepercayaan, pendapatan, dan gender sebagai variabel prediktor. Penelitian ini menggunakan metode survei dalam pengumpulan data. Sebanyak 230 kuesioner dibagikan kepada responden di wilayah mayoritas Muslim (yaitu Kota Surakarta), dari jumlah tersebut, 198 kuesioner yang dapat digunakan. Penelitian ini menggunakan Structural Equation Modelling untuk menganalisis data. Hasil menunjukan bahwa seluruh hipotesis terdukung.
Evaluating the Safety and Efficacy of MSCs-derived Exosomes for Treatment of Refractory Perianal Fistula in IBD patients; Clinical Trial Phase I
Authors: Nazari Hojjatollah , Heirani Asieh , Soleimani Masoud , Asbagh Reza Akbari , hadizadeh Alireza , behboudi behnam , Fazeli Mohammad Sadegh , Rahimi Mojgan , Keramati Mohammad Reza , Keshvari Amir , Kazemeini Alireza , Alborzi Foroogh , tafti seyed mohsen ahmadi 
Published: June 2022

Background and objectives:

Exosomes therapy is a promising technique that can decrease the concern
related to the fate of transplanted stem cells to the fistula location.
The unique properties of mesenchymal stem cells (MSCs)-derived exosomes
can improve fistula healing because of their high ability to regulate
and modify immune responses. This project aims to examine the safety and
efficacy of implementing MSCs-exosomes in treating refractory fistulas
in inflammatory bowel disease (IBD) patients.
Methods:
The exosomes were isolated from umbilical cord-derived MSCs and
characterized using flow cytometry, western blotting, and transmittance
electron microscope (TEM). Five patients (three men and two women) with
refractory perianal Crohn’s disease with an average age of 35.4 years
old were included. The patients did not match the exclusion criteria,
and the presence of refractory fistulas was the main inclusion criteria.
These patients have their exosomes injections in the operation room. The
therapy responses of patients were assessed using physical examination,
face-to-face interviews, computerized tomography (CT) scan, and magnetic
resonance imaging (MRI) six months later.
Result:
The Following injection, patients reported a 70% improvement on
average. Two patients claimed complete healing after exosome injections,
while one patient reported no improvement and active discharge from the
fistula site. In addition, five patients reported no adverse effects,
both systemic or on the injection site.
Conclusion: Injection of exosomes extracted from MSCs shows
total safety and a satisfactory therapeutic impact, as shown in this and
other research, and could play a significant role in the treatment of
gastrointestinal fistulas in the future.
Audit Efficiency and Effectiveness Consequences of Accounting System Homogeneity across Audit Clients: A New Form of Knowledge Spillover?
Authors: Murthy Uday S. , Park Jong Chool , Smith Thomas , Whitworth James 
Published: June 2022

ABSTRACT
We examine the effects of a large number of clients in an audit office using the same enterprise-resource planning (ERP) system such as SAP or Oracle resulting in what we term “client accounting system homogeneity” on audit efficiency and effectiveness. Using a unique dataset of ERP system implementations, we find that accounting system homogeneity is positively associated with audit efficiency. Specifically, we find lower (higher) audit fees for clients using an ERP system from a vendor used by a higher (lower) proportion of clients in that office. We further document that accounting system homogeneity is associated with improved audit effectiveness as proxied by two accruals-based measures, incorrect internal control weakness reporting, and restatements. Our findings are reflective of a new form of knowledge spillover from repeated experiences auditing clients using similar accounting systems, resulting in audits that are not only less expensive but also of higher quality.
Data Availability: Contact the authors.
Maternal Dietary Glycemic Index and Glycemic Load in Pregnancy and Offspring Cord Blood DNA Methylation
Authors: Küpers Leanne K. , Fernández-Barrés Sílvia , Mancano Giulia , Johnson Laura , Ott Raffael , Vioque Jesus , Colombo Marco , Landgraf Kathrin , Tobi Elmar W. , Körner Antje , Gaillard Romy , de Vries Jeanne H.M. , Jaddoe Vincent W.V. , Vrijheid Martine , Sharp Gemma C. , Felix Janine F. 
Published: June 2022

OBJECTIVESuboptimal nutrition in pregnancy is associated with worse offspring cardiometabolic health. DNA methylation may be an underlying mechanism. We meta-analyzed epigenome-wide association studies (EWAS) of maternal dietary glycemic index and load with cord blood DNA methylation.RESEARCH DESIGN AND METHODSWe calculated maternal glycemic index and load from food frequency questionnaires and ran EWAS on cord blood DNA methylation in 2,003 mother-offspring pairs from three cohorts. Analyses were additionally stratified by maternal BMI categories. We looked-up the findings in EWAS of maternal glycemic traits and BMI as well as in EWAS of birth weight and child BMI. We examined associations with gene expression in child blood in the online Human Early Life Exposome eQTM catalog and in 223 adipose tissue samples.RESULTSMaternal glycemic index and load were associated with cord blood DNA methylation at 41 cytosine-phosphate-guanine sites (CpGs, P &lt; 1.17 × 10−7), mostly in mothers with overweight/obesity. We did not observe overlap with CpGs associated with maternal glycemic traits, BMI, or child birth weight or BMI. Only DNA methylation at cg24458009 and cg23347399 was associated with expression of PCED1B and PCDHG, respectively, in child blood, and DNA methylation at cg27193519 was associated with expression of TFAP4, ZNF500, PPL, and ANKS3 in child subcutaneous adipose tissue.CONCLUSIONSWe observed multiple associations of maternal glycemic index and load during pregnancy with cord blood DNA methylation, mostly in mothers with overweight/obesity; some of these CpGs were associated with gene expression. Additional studies are required to further explore functionality, uncover causality, and study pathways to offspring health.
Autoantibodies to Perilipin-1 define a subset of acquired generalized lipodystrophy
Authors: Mandel-Brehm Caleigh , Vazquez Sara E. , Liverman Christopher , Cheng Mickie , Quandt Zoe , Kung Andrew F. , Parent Audrey , Miao Brenda , Disse Emmanuel , Cugnet-Anceau Christine , Dalle Stéphane , Orlova Elizaveta , Frolova Elena , Alba Diana , Michels Aaron , Oftedal Bergithe E. , Lionakis Michail S. , Husebye Eystein S. , Agarwal Anil K. , Li Xilong , Zhu Chengsong , Li Quan , Oral Elif , Brown Rebecca , Anderson Mark S. , Garg Abhinmanyu , DeRisi Joseph L. 
Published: June 2022

<p> </p>
<p>Acquired lipodystrophy is often characterized as an idiopathic subtype of lipodystrophy. Despite suspicion of an immune-mediated pathology, biomarkers such as autoantibodies are generally lacking. Here, we used an unbiased proteome-wide screening approach to identify autoantibodies to the adipocyte-specific lipid droplet protein Perilipin-1 (PLIN1) in a murine model of Autoimmune Polyendocrine Syndrome 1 (APS1). We then tested for PLIN1 autoantibodies in human subjects with acquired lipodystrophy with two independent severe breaks in immune tolerance (including APS1) along with controls using a specific Radioligand Binding Assay and indirect immunofluorescence on fat tissue. We identified autoantibodies to PLIN1 in these two cases, including the first reported case of APS1 with acquired lipodystrophy and a second patient who acquired lipodystrophy as an immune-related adverse event following cancer immunotherapy. Lastly, we also found PLIN1 autoantibodies to be specifically enriched in a subset of patients with acquired generalized lipodystrophy (17/46; 37%) particularly those with panniculitis and other features of autoimmunity. These data lend additional support to new literature that suggests that PLIN1 autoantibodies represent a marker of acquired autoimmune lipodystrophies and further link them to a break in immune tolerance.</p>