bluuebunny/crossref_metadata_2025_split huggingface dataset:
The APC/CTAD1-WIDE LEAF 1-NARROW LEAF 1 pathway controls leaf width in rice
Authors: You Jing , Xiao Wenwen , Zhou Yue , Shen Wenqiang , Ye Li , Yu Peng , Yu Guoling , Duan Qiannan , Zhang Xinfang , He Zhifeng , Xiang Yan , Sang Xianchun , Li Yunfeng , Zhao Fangming , Ling Yinghua , He Guanghua , Zhang Ting 
Published: July 2022

Abstract
Leaf morphology is one of the most important features of the ideal plant architecture. However, the genetic and molecular mechanisms controlling this feature in crops remain largely unknown. Here, we characterized the rice (Oryza sativa) wide leaf 1 (wl1) mutant, which has wider leaves than the wild-type due to more vascular bundles and greater distance between small vascular bundles. WL1 encodes a Cys-2/His-2-type zinc finger protein that interacts with Tillering and Dwarf 1 (TAD1), a co-activator of the anaphase-promoting complex/cyclosome (APC/C) (a multi-subunit E3 ligase). The APC/CTAD1 complex degrades WL1 via the ubiquitin-26S proteasome degradation pathway. Loss-of-function of TAD1 resulted in plants with narrow leaves due to reduced vascular bundle numbers and distance between the small vascular bundles. Interestingly, we found that WL1 negatively regulated the expression of a narrow leaf gene, NARROW LEAF 1 (NAL1), by recruiting the co-repressor TOPLESS-RELATED PROTEIN and directly binding to the NAL1 regulatory region to inhibit its expression by reducing the chromatin histone acetylation. Furthermore, biochemical and genetic analyses revealed that TAD1, WL1, and NAL1 operated in a common pathway to control the leaf width. Our study establishes an important framework for understanding the APC/CTAD1–WL1–NAL1 pathway-mediated control of leaf width in rice, and provides insights for improving crop plant architecture.
Asset Specificity of Nonfinancial Firms
Authors: Kermani Amir , Ma Yueran 
Published: July 2022

AbstractWe develop a new data set to study asset specificity among nonfinancial firms. Our data cover the liquidation values of each category of assets on firms’ balance sheets and provides information across major industries. First, we find that nonfinancial firms have high asset specificity. For example, the liquidation value of fixed assets is 35% of the net book value in the average industry. Second, we analyze the determinants of asset specificity and document that assets’ physical attributes (e.g., mobility, durability, and customization) play a crucial role. Third, we investigate several implications. Consistent with theories of investment irreversibility, high asset specificity is associated with less disinvestment and stronger effects of uncertainty on investment activities. We also find that the increasing prevalence of intangible assets has not significantly reduced firms’ liquidation values.
Duration of Exposure to General Endotracheal Anesthesia During Cesarean Deliveries at Term and Perinatal Complications
Authors: Swanson Kate , Liang Luzhou , Grobman William A. , Higgins Nicole , Roy Archana , Son Moeun 
Published: July 2022

(Abstracted from Am J Perinatol 2022;39:232–237)
General endotracheal anesthesia (GETA) is performed in approximately 6% of cesarean deliveries (CDs). It is less frequently performed than neuraxial anesthesia because of the risk of neonatal complications such as the need for intubation or supplemental oxygen and lower 5-minute Apgar scores.
Risk Factors for Developing Concurrent Posttraumatic Stress Injury After Work-Related Musculoskeletal Injury
Authors: Gross Douglas P. , Rachor Geoffrey S. , Krebs Brandon K. , Yamamoto Shelby S. , Dick Bruce D. , Brown Cary A. , Asmundson Gordon J. G. , Straube Sebastian , Els Charl , Jackson Tanya D. , Brémault-Phillips Suzette , Voaklander Don , Stastny Jarett , Berry Theodore 
Published: July 2022

Objective
This study aimed to study risk factors for developing concurrent posttraumatic stress injury (PTSI) among workers experiencing work-related musculoskeletal injury (MSI).

Methods
A case-control study was conducted using workers' compensation data on injured workers undergoing rehabilitation programs for concurrent MSI and PTSI (cases) and MSI only (controls). A variety of measures known at the time of the compensable injury were entered into logistic regression models.

Results
Of the 1948 workers included, 215 had concurrent MSI and PTSI. Concurrent MSI and PTSI were predicted by type of accident (adjusted odds ratio [OR], 25.8), experiencing fracture or dislocation fracture or dislocation (adjusted OR, 3.7), being public safety personnel (adjusted OR, 3.1), and lower level of education (adjusted OR, 1.9).

Conclusions
Experiencing a concurrent PTSI diagnosis with MSI after work-related accident and injury appears related to occupation, type of accident, and educational background.
The Association Between Forceful Hand Exertions and Musculoskeletal Disorders of the Neck and Shoulder
Authors: Zimbalist Alexa , Rempel David , Feng Lydia , Harris-Adamson Carisa 
Published: July 2022

Objective
The aim of this study was to estimate associations between biomechanical exposures and workplace neck and shoulder musculoskeletal disorders (NSMSDs) in a prospective cohort.

Methods
Manufacturing workers (N = 349) were followed for 28 months. Biomechanical exposures were collected on each individual, and physical examinations were performed every 4 months. Prevalent cases at baseline (n = 30) were excluded from the analysis.

Results
There were 18 incident cases of NSMSDs. Greater than 33% time in forceful hand exertion doubled the rate of incident NSMSDs. Greater than 18 degrees of shoulder abduction, during forceful hand exertion, increased the rate by 55%. Confidence intervals were wide.

Conclusions
Effect estimates of the relationship between forceful hand exertions and NSMSDs were consistently elevated whether measured in isolation or while in shoulder abduction. Following a larger cohort with more frequent physical examinations would improve estimates of the model.
Role of Distraction Osteogenesis in the Management of Postankylotic Deformity
Authors: Anehosur Venkatesh , Monis Prashanth L. , Nagaraj Nikhil , Krishnamurthy Keerthana , Shettigar Pavithra , Patil Anand 
Published: July 2022

Introduction:
Distraction osteogenesis (DO) has evolved in maxillofacial surgery and gained popularity due to the limitations of orthognathic surgery in gross asymmetry cases.

Purpose:
The primary aim of the paper was to determine if the use of DO for the management of severe deformities of the mandible secondary to temporomandibular joint ankylosis can achieve optimal results, with or without genioplasty, based on cephalometric norms. The secondary aim of this study was to evaluate the complications associated with DO in this group of patients.

Materials and Methods:
Six patients with postankylotic deformity were included. Both internal and external devices were used for an average mandibular corpus lengthening of 15 mm with adjunctive procedure genioplasty.

Results:
All patients had excellent outcome in terms of profile, functional occlusion, and mouth opening without deviation. Two patients had primary genioplasty showed excellent compliance and 2 as secondary at the time of device removal. Complications noted were, difficulty in vector control during distraction, paresthesia, occlusal discrepancy, scarring, and relapse of 2 to 3 mm. Preorthodontics and postorthodontics were done in all patients to settle the occlusion, which was stable after 2-year follow-up.

Conclusions:
Case series shows such gross deformities pose a greater challenge due to undergrowth, gross midline shift, and discrepancy between soft and hard tissue growth pattern and the desired results can be achieved by precise planning. Genioplasty at the time of device placement has definitive positive effect on patient compliance. Though orthognathic surgery has a definitive role in minor deformity, whereas in gross deformities DO with precise planning using 3-dimensional model and vector controlled technique offers excellent outcome.
Functional integrity of visual coding following advanced photoreceptor degeneration
Authors: Rodgers Jessica , Hughes Steven , Lindner Moritz , Allen Annette E , Ebrahimi Aghileh , Storchi Riccardo , Peirson Stuart N , Lucas Robert J , Hankins Mark W 
Published: July 2022

AbstractPhotoreceptor degeneration sufficient to produce severe visual loss often spares the inner retina. This raises the hope that treatments using optogenetics or electrical stimulation, which generate a replacement light input signal in surviving neurons, may restore vision. The success of these approaches is dependent on the capacity of surviving circuits in the early stages of the visual system to generate and propagate an appropriate visual code in the face of neuroanatomical remodelling. To determine the capacity of surviving circuits in advanced retinal degeneration to present an appropriate visual code, we generated a transgenic mouse expressing the optogenetic actuator ReaChR in ON bipolar cells (second order neurons in the visual projection). After crossing this with the rd1 model of photoreceptor degeneration, we compared ReaChR derived responses with photoreceptor-driven responses in wildtype (WT) mice in retinal ganglion cells and visual thalamus. The ReaChR-driven responses in rd1 animals showed low photosensitivity, but in other respects generated a visual code that was very similar to WT. Furthermore, ReaChR rd1 units in the retina had high response reproducibility and showed sensitivity normalisation to code contrast stably across different background intensities. At the single unit level, ReaChR-derived responses exhibited broadly similar variation in light response polarity, contrast sensitivity and temporal frequency tuning as WT. Units from WT and ReaChR rd1 mice clustered together when subjected to unsupervised community detection based on stimulus-response properties. Our data reveal an impressive ability for surviving circuitry to recreate a rich visual code following advanced retinal degeneration and are promising for regenerative medicine in the central nervous system.
Development of Equine Polyclonal Antibodies as a Broad-Spectrum Therapy Against SARS-CoV-2 Variants
Authors: Liao Shumin , He Yunjiao , Qu Jing , Shi Yue , Liu Yingzi , Zhao Keli , Chen Junhui , Jing Yue , Shen Clifton Kwang-Fu , Ji Chong , Luo Guxun , Zhao Xusheng , Li Shuo , Fan Yunping , Lv Ziquan , Fang Shisong , He Yaqing , Wu Chunli , Zhang Renli , Zou Xuan , Wang Peng , Li Liang 
Published: July 2022

AbstractThe Coronavirus disease 19 (COVID-19) pandemic has accumulated over 550 million confirmed cases and more than 6.34 million deaths worldwide. Although vaccinations has largely protected the population through the last two years, the effect of vaccination has been increasingly challenged by the emerging SARS-CoV-2 variants. Although several therapeutics including both monoclonal antibodies and small molecule drugs have been used clinically, high cost, viral escape mutations, and potential side effects have reduced their efficacy. There is an urgent need to develop a low cost treatment with wide-spectrum effect against the novel variants of SARS-CoV-2.Here we report a product of equine polyclonal antibodies that showed potential broad spectrum neutralization effect against the major variants of SARS-CoV-2. The equine polyclonal antibodies were generated by horse immunization with the receptor binding domain (RBD) of SARS-CoV-2 spike protein and purified from equine serum. A high binding affinity between the generated equine antibodies and the RBD was observed. Although designed against the RBD of the early wild type strain sequenced in 2020, the equine antibodies also showed a highly efficient neutralization capacity against the major variants of SARS-CoV-2, including the recent BA.2 Omicron variant (IC50 =1.867μg/ml) in viral neutralization assay in Vero E6 cells using live virus cultured. The broad-spectrum neutralization capacity of the equine antibodies was further confirmed using pseudovirus neutralization assay covering the major SARS-CoV-2 variants including wild type, alpha, beta, delta, and omicron, showing effective neutralization against all the tested strains.Ex vivoreconstructed human respiratory organoids representing nasal, bronchial, and lung epitheliums were employed to test the treatment efficacy of the equine antibodies. Antibody treatment protected the human nasal, bronchial, and lung epithelial organoids against infection of the novel SARS-CoV-2 variants challenging public health, the Delta and Omicron BA.2 isolates, by reducing >95% of the viral load. The equine antibodies were further tested for potential side effects in a mouse model by inhalation and no significant pathological feature was observed.Equine antibodies, as a mature medical product, have been widely applied in the treatment of infectious diseases for more than a century, which limits the potential side effects and are capable of large scale production at a low cost. A cost-effective, wide-spectrum equine antibody therapy effective against the major SARS-CoV-2 variants can contribute as an affordable therapy to cover a large portion of the world population, and thus potentially reduce the transmission and mutation of SARS-CoV-2.
A direct interaction between CPF and Pol II links RNA 3ʹ-end processing to transcription
Authors: Carminati Manuel , Manav M. Cemre , Bellini Dom , Passmore Lori A 
Published: July 2022

AbstractTranscription termination by RNA Polymerase II (Pol II) is linked to RNA 3ʹ-end processing by the cleavage and polyadenylation factor (CPF). CPF contains endonuclease, poly(A) polymerase and protein phosphatase activities that cleave and polyadenylate the pre-mRNA, and dephosphorylate Pol II to control transcription. Exactly how the RNA 3ʹ-end processing machinery is coupled to transcription remains unclear. Here, we combine in vitro reconstitution, electron cryo-microscopy and X-ray crystallography to show that CPF physically and functionally interacts with Pol II. Surprisingly, CPF-mediated dephosphorylation promotes the formation of a Pol II stalk-to-stalk homodimer. This dimer is compatible with transcription (unlike other Pol II dimers) but not with the binding of transcription elongation factors. We show that the Ref2 subunit of CPF contributes directly to the interaction with Pol II and also regulates the Glc7/PP1 CPF phosphatase. We hypothesize that Pol II dimerization may play a role in gene looping and may provide a mechanistic basis for the allosteric model of transcription termination.
A gene expression control technology for cell-free systems and synthetic cells via targeted gene silencing and transfection
Authors: Sato Wakana , Rasmussen Melanie , Gaut Nathaniel , Devarajan Mahima , Stokes Kaitlin , Deich Christopher , Engelhart Aaron E. , Adamala Katarzyna P. 
Published: July 2022

AbstractCell-free transcription-translation (TXTL) is an in vitro protein expression platform. In synthetic biology, TXTL is utilized for a variety of technologies, such as genetic circuit construction, metabolic pathway optimization, and building prototypes of synthetic cells. For all these purposes, the ability to precisely control gene expression is essential. Various strategies to control gene expression in TXTL have been developed; however, further advancements on gene-specific and straightforward regulation methods are still demanded. Here, we designed a novel method to control gene expression in TXTL, called a “silencing oligo.” The silencing oligo is a short oligonucleotide that binds to the target mRNA. We demonstrated that addition of the silencing oligo inhibits eGFP expression in TXTL in a sequence-dependent manner. We investigated one of the silencing oligo’s inhibitory mechanisms and confirmed that silencing is associated with RNase H activity in bacterial TXTL reactions. We also engineered a transfection system that can be used in synthetic cells. We screened two dozen different commercially available transfection reagents to identify the one that works most robustly in our system. Finally, we combined the silencing oligo with the transfection technology, demonstrating that we can control the gene expression by transfecting silencing oligo-containing liposomes into the synthetic cells.
Global trends in board diversity research: a bibliometric view
Authors: Khatib Saleh F.A. , Abdullah Dewi Fariha , Elamer Ahmed , Yahaya Ibrahim Suleiman , Owusu Andrews 
Published: July 2022

Purpose
This study aims to identify the main research development on board diversity and offers a quantitative synopsis of key themes and contributors, knowledge gaps and provides directions for further work.

Design/methodology/approach
Using a bibliometric analysis, the authors assess the patterns in global board diversity research based on co-occurrences of researchers’ keywords and publication outputs of 991 articles from the Scopus database. Also, the co-citation network analysis was performed to assess the intellectual structure of board diversity research.

Findings
According to the keyword analysis, the authors found that researchers focus on the gender diversity of the boardroom while ignoring the cognitive diversity and other aspects of demographic diversity such as educational, ethnic, age, nationality, experience, background and tenure, pointing to the need for further work to consider other diversity attributes and the interaction between them. Additionally, board diversity research related to (but not limited to) payout policy, cash holding, initial public offerings, small–medium enterprises and financial institutions is limited.

Originality/value
This study provides a comprehensive evaluation of the development of board diversity research (using a large archival database) and identifies the common construct as well as the potential opportunities for future research directions.
Effect of chitosan on cardio-metabolic risk factors induced by high fat diet in rats
Authors: Ugbaja Regina Ngozi , Onunkwor Beno Okechukwu , Ugwor Emmanuel Ifeanyichukwu , Ogungbemi Kunle , Adewole Anuoluwapo , Olowoyeye Michael Ayobami , Arowojolu David , Adeleke Olamide Yewande , Ugbaja Victory Chukwudalu 
Published: July 2022

PurposeThis study aims to scrutinize the efficacy of chitosan (CT) on cardio-lipotoxic responses elicited by a high-fat diet (HF).Design/methodology/approachThirty-six male Wistar rats were distributed across six groups (n= 6): normal diet (ND), HF, ND-5%CT, HF-1%CT, HF-3%CT and HF-5%CT, for seven weeks. Blood and cardiac tissues were processed for biochemical, immunohistochemical and histopathological analyses.FindingsIngestion of HF induced hyperlipidaemia and lipid accumulation, leading to increased body and heart weight by 70.5% (p< 0.0001) and 124% (p= 0.0021), respectively, compared to ND-groups. Cardiac damage markers (creatine kinase, lactate dehydrogenase and malondialdehyde) were higher in the HF-group compared to control rats. Also, atherogenic and coronary risk indices were significantly elevated by 155% (p= 0.0044) and 174% (p= 0.0008), respectively, compared to control rats. Rats fed HF had significantly reduced cardiac antioxidants (superoxide dismutase, catalase and glutathione peroxidase) and elevated expression of NF-κB-p65 and p53 (p< 0.0001) in the cardiac tissues. Histology revealed lipid inclusions in the cardiac tissues of HF-groups. CT (1%–5%) prevented hyperlipidaemia, lipid accumulation, oxidative stress and cardiac damage in HF-fed rats, while greatly improving the histology of the cardiac tissues in HF-fed rats in a dose-dependent manner.Originality/valueTo the best of the authors’ knowledge, this is the first report of the effects of CT against cardio-lipotoxicity elicited by HF diet ingestion. The findings suggest that CT may present a safe therapeutic alternative for managing complications arising from cardio-lipotoxicity.
Size premium or size discount? – A dynamic capital mobility based interpretation
Authors: Song Garrison Hongyu 
Published: July 2022

Purpose
The size effect that there exist return differences between small market-cap firms and large market-cap counterparts in the stock market has become one of the most controversial capital market anomalies. This paper aims to interpret this effect, including both the size premium and the size discount.

Design/methodology/approach
A dynamic capital mobility model (DCMM) is proposed, and the model’s explanatory ability is validated via simulation.

Findings
This study’s simulation results indicate that the observed size effect can be originated from the combination of the pure size effect and the investors’ herding behavior. Although the size premium, that average returns of small firms are higher than those of large firms, is more prevalent in the stock market, this study’s model implies that the size discount is also possible, which is largely an empirical issue. The pure size effect per se cannot reproduce the size premium. Only if the herding effect dominates the pure size effect would there exist the size premium.

Originality/value
Although the literature provides miscellaneous explanations for the size effect, they are still inconclusive. So far there has been no theory to directly investigate the size effect and to explicitly explore the impact of investors’ trading behavior on the size effect. To the best of the author’s knowledge, this paper fills in this gap and proposes a DCMM to interpret the size effect for the first time. In addition, while the literature focuses on the size premium only, this study covers not only the size premium but also the size discount.
Sustainable food cold chain logistics: From microenvironmental monitoring to global impact
Authors: Chen Qian , Qian Jianping , Yang Han , Wu Wenbin 
Published: July 2022

AbstractFood cold chain logistics (FCCL) is a systematic engineering process involving the use of a low‐temperature environment to maintain the quality and safety of perishable food and reduce food loss and waste (FLW). From a mechanism perspective, FCCL must balance resource costs for a required level of food quality and safety with the costs of greenhouse gas (GHG) emissions. In the context of global warming, the sustainability trade‐off between FLW and environmental impact has recently become an important topic in research on efficient, green FCCL. This is mainly reflected in technological innovation, management optimization, and policy responses. With a focus on three levels (micro, meso, macro), this review analyzes current research areas and the gaps and challenges of FCCL in microenvironmental monitoring, life cycle assessment (LCA), and global impact. Future trends pertaining to FCCL in technology, management, and industry and sustainable development are also summarized. Future trends involving sustainable FCCL must be intelligent, systematic, and low carbon. Industry empowerment through next‐generation information technologies (e.g., IoT, AI, big data, blockchain) will promote the multidimensional perception, real‐time information transmission, and sustainable control of microenvironmental monitoring, as well as support LCA management transformation from fragmentation to system integration. From a macro level, due to the serious global loss of perishable food, the FCCL scale demand is growing greatly, causing a huge environmental burden. Global cooperation, low‐carbon consensus, and appropriate policies will become the basis for promoting sustainable FCCL development.
Clinical and histopathological features of onychopapilloma in an Australian setting: A case series of 50 patients
Authors: Yun Jenny S. W. , Howard Anne , Prakash Saurabh , Kern Johannes S. 
Published: July 2022

AbstractOnychopapilloma is an uncommon tumour of the nailbed and the distal nail matrix. To date, only 19 case reports and case series have been reported in the literature. This article includes literature review on all reported cases and provides the first case series of onychopapilloma in an Australian population, evaluating the clinical features and histopathological diagnosis of patients with onychopapilloma in an Australian subspecialty nail clinic.