bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mentee-mentor Satisfaction among Junior Doctors and Senior Medical Staff at Two Australian hospitals: A Longitudinal Study of a Mentoring Program.
Authors: David Michael C. 
Published: March 2022

Abstract
BackgroundMentoring is a unique educational workplace relationship that can support the skill, knowledge, social and emotional needs of both the mentee and mentor. This study aimed to assess the level of participant satisfaction in achieving these aims for a mentoring program implemented at two hospitals in New South Wales, Australia. MethodsUsing a quantitative approach, survey data were collected from 22 junior doctors (the mentees) and 21 senior medical staff (the mentors) at pre-, mid-and post-program. Between group differences were assessed using the Mann-Whitney U test and Fishers’ exact test, while temporal trend was assessed using the Cochran-Armitage trend test. Results Though no temporal trends or mentee-mentor differences reached statistical significance, junior doctors were found to initiate mentee-mentor meetings more frequently, at both mid-program (50% vs 30%) and post-program (50% vs 19). In addition mentees displayed higher levels of program satisfaction when compared to their mentors at mid-program (100% vs 85%) and post-gram (94% vs 75%). ConclusionsMentoring, if implemented correctly and well-timed, undoubtedly has the necessary properties to enhance each participating party’s professional and personal lives, be they doctors or not.
Endogenous oxytocin levels in extracted saliva elevates during breastfeeding correlated with lower postpartum anxiety in primiparous mothers
Authors: Nagahashi-Araki Miyuki , Tasaka Makoto , Takamura Tsunehiko , Eto Hiromi , Sasaki Noriko , Fujita Wakako , Miyazaki Asuka , Morifuji Kanako , Honda Naoko , Miyamura Tunetake , Shota Nishitani 
Published: March 2022

Abstract
Background
Breastfeeding in the early postpartum period is expected to have mental benefits for mothers; however, the underlying psychobiological mechanisms remain unclear. Previously, we hypothesized that the release of oxytocin in response to the suckling stimuli during breastfeeding would mediate a calming effect on primiparous mothers, and we examined salivary oxytocin measurements in primiparous mothers at postpartum day 4 using saliva samples without extraction, which was erroneous. Thus, further confirmation of this hypothesis with a precise methodology was needed.
Methods
We collected saliva samples at three time points to measure oxytocin in 24 primiparous mothers on postpartum day 2 (PD2) and 4 (PD4) across the breastfeeding cycle (before, during, and after breastfeeding). Salivary oxytocin levels using both extracted and unextracted method were measured and compared to show the qualitative differences. State and trait anxiety and clinical demographics were evaluated to determine their association with oxytocin changes.
Results
Breastfeeding elevated salivary oxytocin levels at PD2; however, there was no significant increase at PD4 in the extracted method. We found a weak but significant positive correlation between extracted and unextracted oxytocin levels during breastfeeding; there were no other significant positive correlations. Therefore, we used the extracted measurement index for subsequent analysis. We showed that the greater the increase in oxytocin during breastfeeding, the lower the state anxiety, but not trait anxiety. Mothers who exclusively breastfed at the 1-month follow-up had a slightly higher oxytocin change at PD2 compared to those who did not.
Conclusions
Breastfeeding in early postpartum days could be accompanied by frequent release of oxytocin and lower state anxiety, potentially contributing to exclusive breastfeeding.
Glans diameter and meatus localization are the sole predictors of primary distal hypospadias surgery complications: A multivariate analysis of single surgeon series.
Authors: Akova Fatih , Bilgili Zeynep , Aydın Emrah , Salabas Emre 
Published: March 2022

Abstract
Purpose
Tubularized incised plate urethroplasty (TIPU) surgery is among most successful techniques for distal hypospadias. Our objective was the investigation of complication rates and their predictors.
Methods
Between 2010–2021, 150 patients with distal hypospadias were operated consecutively by a single surgeon using TIPU technique. The primary outcome was the complication rates including fistula, meatal stenosis, glans dehiscence. Secondary outcomes were predictor factors of complications.
Results
Glans diameter average was 13.9 + 0.10 mm and 57.0% of the patients had a glans diameter greater than 14 mm. Single-layer and double-layer urethroplasty were used in 55.3%(n = 83) and 44.7%(n = 67) of patients respectively. Overall complication rate was 23.3%(n = 35) which included fistula (3.3%,n = 5), glans dehiscence (12.7%, n = 19) and meatal stenosis (8.6%,n = 13). Glandular meatus localization (OR 58.8, p = 0.001) and smaller glans diameter (OR 0.39,p = 0.001) were significant predictors in multivariate analysis of overall complications. For fistula complication, only short operation time (OR 0.83, p = 0.03) was found as a significant predictor. Glans width (< 14 mm) was the only significant predictor of both glans dehiscence (OR 3.4,p = 0.03) and stenosis (OR 5.67,p = 0.013) complication.
Conclusion
TIPU technique for distal hypospadias has notable success and acceptable complication rates. Dartos augmented single layer urethral closure seems adequate for complication prevention. Preoperative assessment of glans width, meatus-site is advised to predict complication rates.
Exosomes-Based Humoral Regulation of Iron Metabolism is Required for Host Antibacterial Response
Authors: Kuang Huijuan , Dou Geng , Cheng Linfeng , Wang Xiangdong , Xu Haokun , Liu Xuemei , Ding Feng , Yang Xiaoshan , Liu Siying , Bao Lili , Liu Huan , Li Bei , Jin Yan , Liu Shiyu 
Published: March 2022

Abstract
Immediate iron restriction by the host is a critical process to protect against bacterial infections. Although the cell-dependent iron sequestration mechanism in liver or spleen has been identified, it is still unclear that whether the host launched humoral regulation mechanism to promptly acquire iron that widely distributes throughout body fluids. Here, we showed that after bacterial invasion, host immediately releases nanosized exosomes to capture circulating iron-containing proteins, which is required for prompt systemic iron sequestration and antibacterial defense. Mechanistically, in a sepsis model, we found that Salmonella Typhimurium induces endoplasmic reticulum stress in macrophages and activates inositol requiring enzyme 1α (IRE1α) signaling, triggering lysosomal dysfunction and promoting exosome release. These exosomes bearing transferrin receptors, CD163 and CD91 bind multiple iron-containing proteins, prevent bacteria from iron acquisition, and recycle them to tissue-resident macrophages, ultimately sequestering iron and protecting against infection. Our findings reveal a previously unknown humoral regulation mechanism of iron metabolism during bacterial infection, and suggest the release and circulation of extracellular vesicles could be an important way to promptly regulate systemic ion metabolism.
Coexistence of Systemic Sclerosis and Microscopic Polyangitis Associated With Pulmonary Renal Syndrome: a Case Report and Literature Review
Authors: jiang Yi , Liao Xia , He Yixi , Lei Tianyi , Wang Shunbing , Qing Yufeng 
Published: March 2022

Abstract
Objective: To investigate the clinical characteristics, diagnosis and treatment of coexistence of systemic sclerosis (SSc) and microscopic polyangitis（MPA）associated with pulmonary renal syndrome (PRS).Methods: The clinical data, diagnosis and treatment process of a patient who has SSC combined with MPA and PRS were summarized and analyzed. And the literature was reviewed to explore the correlation of the pathogenesis and clinical experience of SSC complicated with MPA and PRS. Results: The case is a middle-aged male who was diagnosed as SSc due to the hardening of the skin of both hands, Reynolds phenomenon, the anti-scl-70 antibody are positive. The patient suffered from repeated hemoptysis, progressive dyspnea, severe anemia and renal insufficiency, so he was diagnosed as MPA with PRS. After giving glucocorticoid, immunosuppressant and anti-infection treatment, his condition has improved. A total of 7 case reports were retrieved by reviewing the relevant literature.A total of 9 patients were reported. They were first diagnosed as SSc and then MPA with PRS, of which 4 cases improved after treatment and 5 cases died. Among the dead patients, 3 case was treated with D-penicillamine, and the remaining 2 cases were only treated with steroids or hemodialysis. The three have a certain correlation in pathogenesis. Glucocorticoids, immunosuppressants, biological agents, hemodialysis and plasma exchange are the main treatments.
Maternal plasma proteome profiling of biomarkers and pathogenic mechanisms of early-onset and late-onset preeclampsia
Authors: Chen Hao , Aneman Ingrid , Nikolic Valentina , Orlic Natasa Karadzov , Mikovic Zeljko , Stefanovic Milan , Cakic Zoran , Jovanovic Hristina , Town Stephanie , Padula Matthew , McClements Lana 
Published: March 2022

Abstract
BackgroundPreeclampsia is still the leading cause of morbidity and mortality in pregnancy, however there are no current effective therapeutic strategies. This has been impeded by poorly understood pathogeneses of preeclampsia and its multifactorial and heterogeneous nature. Two phenotypes of preeclampsia have been characterised based on the time of diagnosis, early-onset (EOPE, before 34 weeks’ of gestation) and late-onset (LOPE, from 34 weeks’ of gestation). However, the molecular differences between these two phenotypes are not fully elucidated. ObjectivesThis study aimed to facilitate better understanding of the mechanisms of pathogenesis of EOPE and LOPE, and identify specific biomarkers of preeclampsia.MethodsIn this study, we conducted an untargeted proteomic analyses of plasma samples from pregnant women with EOPE (n=17) and LOPE (n=11), and age, BMI-matched normotensive controls (n=18).ResultsIn total, there were 26 and 20 differentially abundant proteins between EOPE or LOPE, and normotensive controls, respectively. Only inter-alpha-trypsin inhibitor heavy chain 3 (ITIH3) was differentially abundant and 11 proteins were commonly shared between EOPE and LOPE. A series of angiogenic proteins, including insulin-like growth factor-binding protein 4 (IGFBP4; EOPE: FDR =0.30 x 10-3 and LOPE: FDR =3.96 x 10-3) and histidine-rich glycoprotein (HRG), were significantly perturbed in both phenotypes (EOPE: FDR=7.8 x 10-3; LOPE: FDR =4.1 x 10-3). ConclusionsOverall, proteins associated with lipid metabolism were the key proteins perturbed in EOPE, however, ECM proteins had a more pronounced role in LOPE. The homeostasis-related pathway including platelet activation, signalling and aggregation were more perturbed in EOPE than LOPE.
The Role of Loyalty and Satisfaction’s Effect on Word-of-Mouth Influence in Indonesian B2B Environment
Authors: Syahputra Syahputra , Fakhri Mahendra , Putri Clarissa Aulia , PRADANA MAHIR 
Published: March 2022

Abstract
Customers are active in the service process, therefore the quality of the services they consume is determined by them. These findings suggest that product expectations and values are used to gauge customer satisfaction. The growth of a diverse range of distinctive apparel, as well as clothing trends as people's lifestyles, demonstrates that this industry is rapidly expanding. The research methods employed in this study were quantitative research methodologies. Partial Least Square TStructural Equation Modeling was used to examine this investigation (PLS-SEM). Convergent validity, discriminant validity, and composite reliability are all tested during the measurement model testing phase. The outcomes of the PLS analysis can be utilized to evaluate the study hypothesis if all indicators in the PLS model meet the standards of convergent validity, discriminant value dirt, and reliability testing. The relationship between variables in research is a means of testing specific theories. The importance of selling the company through word of mouth cannot be overstated. By word of mouth, customer satisfaction with the selling company has a favorable and considerable impact on company sales. A reliability test was performed in this study to determine the measurement instrument's internal consistency. The relationship between variables in research is a means of testing specific theories. The importance of selling the company through word of mouth cannot be overstated. Consumers who are happy with the services they receive are more likely to make repeat purchases, to be loyal to the firm, and, most importantly, to spread the word about the company to their friends and family.
Importance of responsible interpretation of observational studies with an impact on public health policy
Authors: Kanankege Kaushi ST , Traynor Isaac , Perez Andres M 
Published: March 2022

Abstract
Observational studies are often referenced when informing policy decisions in public health. However, there limitations associated with the nature of observational studies may lead to erroneous interpretations and recommendations. A peer-reviewed publication published in 2018 reported that communities living near hog Concentrated Animal Feeding Operations (CAFO) have increased negative health outcomes and mortalities, in the U.S. state of North Carolina (NC). While authors stated that associations does not imply causation, speculative interpretation of their results by media caused detrimental effects on the swine industry. The objective here was to repeat their study using updated data and to evaluate the strength of conclusions and appropriateness of methods used with the ultimate goal of alerting on the impact that study limitations may have when used as evidence for decision-making. The referenced manuscript reported results from an ecological study design that used available data from 2007–2013. They analyzed exposure to CAFOs using a logistic regression model conducted at the individual-level, while presumably correcting for six confounding factors. We used same data sources and analytical methods, with additional years of data (2007–2018). Mortality, hospital admissions, and emergency department visits related eight diseases were analyzed (anemia, kidney disease, infectious diseases, tuberculosis, low birth weight, HIV, and diabetes). The study population and their exposure was defined by categorizing NC zip codes into three by pig density; where, > 1 hogs/km2 (G1), > 232hogs/km2 (G2), and no hogs (Control). Our repeated analysis recognized that the early study approach was inadequate to assess the intended hypothesis and had shortcomings including presence of ecological fallacy, residual confounding, inconsistency of associations, and over estimation of exposure. Diseases that are not causally relatable to hog CAFOs, such as HIV and diabetes, were also prominent in the communities neighboring CAFOs (G1 and G2). These associations, in part, reflects an alternative explanation of systemic health disparities of the communities near hog CAFOs. An ecological study would not confirm or deny exposure over time nor indicate causation. Hence, we emphasize the importance of responsible interpretation of ecological studies that concerns public health, agriculture, and economy, for science to provide appropriate and accurate support to the ultimate objective of targeting policy to enhance the quality of life and health of the communities.
Green synthesis, characterization and bioactivity evaluation of Caesium carbonate nanoparticle using Coleus amboinicus
Authors: Ramya K. , Ramalingam R. Jothi , Nethra V. , Zuha S. Shamsiz , Yuvaraj Dinakarkumar , Al-Lohedan Hamad , Arokiyaraj Selvaraj , Alqahtani H. M. 
Published: March 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Investigation on Microstructural and Mechanical Behaviour of Atmospheric Plasma Sprayed Stellite 6 Alloy Coatings on AISI 304 Stainless Steel
Authors: Sarangi Sangita , Mishra Ajit Kumar , Sahoo Seshadev , Bajpai Subhra 
Published: March 2022

Abstract
Stellite 6 alloy has been extensively used in industrial applications to prevent wear and corrosion because of its high strength, high hardness, and corrosion resistance. The wear and frictional properties of a material depend largely on the normal load and hardness of the mating surfaces. Wear resistance characteristics of the material can be improved substantially by reorienting the microstructure of the grain size of the metal along with imparting a higher hardness on the mating surfaces. In the present study, an effort is made to deposit Stellite 6 powder having a regular spherical shape with diameters ranging between 15 µm to 45 µm on an AISI 304 stainless steel substrate without any intermittent layer using an atmospheric plasma spray deposition process. The effect of coating thickness (74 µm, 128 µm, and 215 µm) on metallurgical, morphological, and mechanical characteristics was investigated. Optical microscopy, Scanning Electron Microscopy (SEM), and EDS were employed to study the Stellite 6 coating morphology, and the structural analysis was used to identify the phase formation by using XRD. From the experimental analysis it is observed that, the Stellite 6 coating is uniform and homogenous in nature. Sample coated with Stellite 6 powder having 128 µm coating thickness is exhibiting the best microstructure and mechanical properties as compared to the other two coated samples.
Transcriptome Profiling of Two Hybrid Rice Provides Insights Into the Genetic Basis of Heterosis.
Authors: Fu Jun , Zhang Yilin , Yan Tianze , Li Yanfeng , Jiang Nan , Zhou Yanbiao , Zhou Qunfeng , Qin Peng , Fu Chenjian , Lin Haiyan , Zhong Jing , Han Xue , Lin Zechuan , Wang Fei , He Hang , Wang Kai , Yang Yuanzhu 
Published: March 2022

Abstract
BackgroundHeterosis is a phenomenon that hybrids show superior performance over their parents. The successful utilization of heterosis has greatly improved rice productivity, but the molecular basis of heterosis remains largely unclear. ResultsHere, the transcriptomes of young panicles and leaves of the two widely grown two-line super hybrid rice varieties (Jing-Liang-You-Hua-Zhan (JLYHZ) and Long-Liang-You-Hua-Zhan (LLYHZ)) and their parents were analyzed by RNA-seq. Transcriptome profiling of hybrid revealed 1,778 ~ 9,404 differentially expressed genes (DEGs) in two tissues, which were identified compared with their parents. GO and KEGG enrichment analysis showed that the pathways significantly enriched in both tissues of two hybrids were related to yield and resistance, like circadian rhythm (GO:0007623), response to water deprivation (GO:0009414), photosynthetic genes (osa00196). Allele-specific expression genes (ASEGs) were also identified in hybrids. These ASEGs were most significantly enriched in ionotropic glutamate receptor signaling pathway, which was hypothesized to be potential amino acid sensors in plants. Moreover, the ASEGs were also differentially expressed between parents. The number of variations in ASEGs is higher than expected, especially for large effect variations. These DEGs and ASEGs are the potential reasons for the formation of heterosis in the two combinations. ConclusionsOur results provide a comprehensive understanding of the heterosis of two-line super hybrid rice and facilitate the exploitation of heterosis in hybrid rice breeding with high yield heterosis.
Effect of Hunteria umbellata methanolic seed extracts on streptozotocin-induced diabetic albino rats
Authors: caleb Nwaogwugwu joel , I Nosiri C. , Aguwamba Chinedu 
Published: March 2022

Abstract
This study investigated the inhibitory effect of alpha-amylase, LD50 and antidiabetic properties of the methanolic seed extract of Hunteria umbellata and its effect on biochemical parameters in streptozotocin-induced diabetic rats. Twelve albino rats were used for the acute toxicity test, while thirty-five were divided randomly into seven groups of five in each group. Group 1served as the normal control, Group 2 served as the diabetic control, Group 3 was treated with glycinorm at 50 mg/kg body weight, Group 4 was treated with extract at 200 mg/kg body weight, Group 5 was treated with 400 mg/kg body weight, Group 6was treated with 600 mg/kg body weight and Group 7 was treated with 800 mg/kg body weight by oral administration. Diabetes was induced in albino rats by intraperitoneal injection of streptozotocin at a single dose of 120 mg/kg body weight into groups 3 to 7 and was fed with methanolic seed extract of Hunteria umbellata for a period of 28 days.. The oral acute (LD50) toxicity study showed that extract did not cause mortality in any experimental animals even at the highest dose of 5000 mg/kg. Body weight and glucose levels were measured on days 0, 7, 14,21 and 28. The animal was sacrificed on day 28. Serum biochemical parameters were analysed. Additionally, renal function tests, including potassium, sodium, and chloride levels and protein and albumin levels, were performed. The study also evaluated hematological parameters in the animals that were fed the methanolic seed extract of Hunteria umbellata. The animals that received different methanolic seed extracts of Hunteria umbellata showed significant (P < 0.05) reductions in blood glucose, serum liver enzymes, renal function biomarkers, packed cell volume and platelet counts and improved body weight. Conclusively,from this study, it has been demonstrated that the methanolic seed extract of Hunteria umbellata may possess weight enhancing, antihyperglycemic, hepatoprotective, improved hematological values, and cell and organ protective activities.
Color Stability of Hybrid Ceramics Exposed to Beverages in Different Combinations
Authors: YERLİYURT Kaan , SARIKAYA Işıl 
Published: March 2022

Abstract
Background: The aim of this study is to evaluate the color stability of hybrid ceramics stored in different combinations of different beverages that would be consumed in a daily routine. Methods: The specimens were prepared with resin nano-ceramic (Lava Ultimate, 3M Espe, USA) and hybrid ceramic (Vita Enamic, Vita Zahnfabrik, Germany). The specimens were aged in a thermocycler machine for 10,000 cycles. They were stored in different combinations of beverages (water, tea, coke, coffee, red wine, pomegranate juice, turnip juice) for 12+12 hours-time. The surface roughness measurements were made with a profilometer. The color measurements of the specimens were measured with the spectrophotometer. The color values (L-a-b) of the specimens, and mean surface roughness values (Ra) were recorded at the end of the 1st (D1), 7th (D7), 14th (D14) and 28th (D28) days.Results: When the solution groups are examined; significantly more color changes were observed in the LG4, EG4, LG6 and EG6 groups than in the other groups (p<0.01). Except for the samples in the LG6 (D28) and EG6 groups (D28); more color changes were observed in the Lava samples than the Enamic samples in all groups and periods. Conclusion: More color changes (except for the samples stored in coffee-wine) were observed in the Lava samples than in the Enamic samples in all groups and periods. The combination of coffee-tea and coffee-wine beverages may have accepted most coloring beverages for hybrid ceramics within the limitation of this study.