bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primeiro relato de produção de colagenase por fungo isolado do solo da Caatinga - Rhizopus microsporus UCP 1296
Authors: Ferreira Isabele Albuquerque Alcoforado , Wanderley Maria Carolina de Albuquerque , Porto Ana Lucia Figueiredo 
Published: September 2022

O presente trabalho teve como objetivo estudar o fungo Rhizopus microsporus UCP 1296 isolado do solo da Caatinga enfatizando o desenvolvimento e aplicação de processos biotecnológicos para produção de uma nova colagenase. Para tanto, parâmetros fermentativos significantes como: temperatura, pH, meio de cultura e tempo de fermentação foram avaliados. Amostras do microrganismo foram mantidas a 4 °C em meio ágar extrato de malte. O micélio foi suspenso em 5 mL de uma solução composta de NaCl e Tween 80. Após a inoculação feita com a suspensão de esporos contendo 150 µL (106 esporos/mL), foi realizada a fermentação submersa em frascos Erlenmeyer contendo 250mL de gelatina. Uma curva de crescimento foi desenvolvida para identificar o melhor tempo de produção da enzima. Os resultados que a produção da colagenase ocorreu com 120 horas de fermentação, obtendo-se 550 U/mL de atividade colagenolítica e biomassa 0,42 g/L na fase estacionária, indicando que a colagenase produzida é um metabólito secundário. Um planejamento fatorial 24 foi realizado para selecionar os melhores parâmetros onde foi avaliada a influência das variáveis: temperatura (ºC), pH, agitação e concentração do substrato (gelatina). Com o resultado do planejamento fatorial obtivemos um aumento da produção enzimática cerca de 32%. Este é o primeiro estudo que descreve condições otimizadas de produção de colagenase por cepas de R. microsporus.
Estratégias de promoção da saúde do adolescente desenvolvidas por enfermeiros
Authors: Lins Igor Evangelista Melo , Maia Nívia Maria e Silva , Pinheiro Maria Sabrina da Silva , Amaral João Pedro Maciel , Sá Isadora Ferreira , Oliveira Ismênia Andrade , Silva Vanusa Maria Gomes Napoleão 
Published: September 2022

Objetivo: identificar e analisar a atuação do enfermeiro nas práticas de promoção da saúde do adolescente a partir da literatura científica. Método: revisão integrativa realizada no período de março a abril de 2021. No portal Scielo Brasil e na base BDENF foi utilizada a equação de busca “saúde do adolescente” AND “promoção da saúde’ AND “enfermagem” e na Lilacs, “saúde do adolescente” AND “enfermagem” AND “adolescência”. Resultados: foram incluídos 10 artigos e da análise destes surgiram duas categorias: Desafios profissionais para o desenvolvimento da promoção da saúde do adolescente no âmbito da atenção primária e Estratégias e práticas promotoras da saúde do adolescente. Observou-se como principais resultados a falta de conhecimento dos enfermeiros sobre as diretrizes nacionais para atenção à saúde do adolescente, a sobrecarga de trabalho nos demais programas de saúde e a falta da busca do adolescente à unidade levando a dificuldade de aproximação do enfermeiro com o adolescente. Considerações Finais: verificou-se que muitos enfermeiros reconhecem a importância de promover ações para o público adolescente de sua área, porém ficou evidente que muitos se sentem despreparados para essa promoção. Espera-se que este estudo colabore para que os profissionais de saúde busquem estratégias efetivas de promoção e prevenção voltada para o público adolescente.
A sensitive and rapid method of lead detection using nanoparticle technology based on monoclonal antibody
Authors: Jia Kunzhi , Lin Ming , Zhao Qiang , Dong Mingke , Ling Sumei , Wang Shihua 
Published: September 2022

Lead (Pb) threatens public health due to its toxicity and nonbiodegradable characteristics. It is of significance to develop a sensitive and rapid method for Pb detection. In this study, monoclonal antibodies against Pb were screened with a high affinity constant (Kaff) of 3.56 × 109 L/mol. Au nanosphere particles (AuNS) and Au nanoflower particles (AuNF) were synthesized with a diameter of 15 nm and 60 nm, respectively. The specific anti-Pb antibodies were then immobilized on AuNS and AuNF for probe development. At last, AuNS- and AuNF-based strips were successfully assembled for comparative study, which were able to effectively detect environmental Pb in 10 min. The limits of detection (LODs) were determined to be 3.91 ng/ml and 0.2 ng/ml, respectively. Thus the developed method provides a feasible solution for sensitive and rapid detection of Pb on site, which is beneficial to food safety and pollution control.
Machine learning assisted reflectance spectral characterisation of coronary thrombi correlates with microvascular injury in patients with ST-segment elevation acute coronary syndrome
Authors: Kotronias Rafail A. , Fielding Kirsty , Greenhalgh Charlotte , Lee Regent , Alkhalil Mohammad , Marin Federico , Emfietzoglou Maria , Banning Adrian P. , Vallance Claire , Channon Keith M. , De Maria Giovanni Luigi 
Published: September 2022

AimsWe set out to further develop reflectance spectroscopy for the characterisation and quantification of coronary thrombi. Additionally, we explore the potential of our approach for use as a risk stratification tool by exploring the relation of reflectance spectra to indices of coronary microvascular injury.Methods and resultsWe performed hyperspectral imaging of coronary thrombi aspirated from 306 patients presenting with ST-segment elevation acute coronary syndrome (STEACS). Spatially resolved reflected light spectra were analysed using unsupervised machine learning approaches. Invasive [index of coronary microvascular resistance (IMR)] and non-invasive [microvascular obstruction (MVO) at cardiac magnetic resonance imaging] indices of coronary microvascular injury were measured in a sub-cohort of 36 patients. The derived spectral signatures of coronary thrombi were correlated with both invasive and non-invasive indices of coronary microvascular injury. Successful machine-learning-based classification of the various thrombus image components, including differentiation between blood and thrombus, was achieved when classifying the pixel spectra into 11 groups. Fitting of the spectra to basis spectra recorded for separated blood components confirmed excellent correlation with visually inspected thrombi. In the 36 patients who underwent successful thrombectomy, spectral signatures were found to correlate well with the index of microcirculatory resistance and microvascular obstruction; R2: 0.80, p &lt; 0.0001, n = 21 and R2: 0.64, p = 0.02, n = 17, respectively.ConclusionMachine learning assisted reflectance spectral analysis can provide a measure of thrombus composition and evaluate coronary microvascular injury in patients with STEACS. Future work will further validate its deployment as a point-of-care diagnostic and risk stratification tool for STEACS care.
A systematic review and meta-analysis of the safety and efficacy of left atrial substrate modification in atrial fibrillation patients with low voltage areas
Authors: Mao Shaobin , Fan Hongxuan , Wang Leigang , Wang Yongle , Wang Xun , Zhao Jianqi , Yu Bing , Zhang Yao , Zhang Wenjing , Liang Bin 
Published: September 2022

BackgroundThe left atrial low-voltage areas (LVAs) are associated with atrial fibrosis; however, it is not clear how the left atrial LVAs affect the recurrence of arrhythmias after catheter ablation, and the efficacy and safety of the left atrial substrate modification based on LVAs as a strategy for catheter ablation of atrial fibrillation (AF) are not evident for AF patients with LVAs.MethodsWe performed a systematic search to compare the arrhythmia recurrence in AF patients with and without LVAs after conventional ablation and arrhythmia recurrence in LVAs patients after conventional ablation with and without substrate modification based on LVAs.ResultA total of 6 studies were included, involving 1,175 patients. The arrhythmia recurrence was higher in LVA patients after conventional ablation (OR: 5.14, 95% CI: [3.11, 8.49]; P &lt; 0.00001). Additional LVAs substrate modification could improve the freedom of arrhythmia in LVAs patients after the first procedure (OR: 0.30, 95% CI: [0.15, 0.62]; P = 0.0009). However, there was no significant difference after multiple procedures (P = 0.19). The procedure time (MD: 26.61, 95% CI [15.79, 37.42]; P &lt; 0.00001) and fluoroscopy time (MD: 6.90, 95% CI [4.34, 9.47]; P &lt; 0.00001) in LVAs patients with additional LVAs substrate modification were significantly increased compared to LVAs patients’ without substrate modification. Nevertheless, there were no higher LVAs substrate modification-related complications (P = 0.93) between LVAs patients with and without additional LVAs substrate modification. In the subgroup analysis, the additional LVAs substrate modification reduced the risk of arrhythmia recurrence in LVAs patients during the follow-up time, which was 12 months (OR: 0.32, 95% CI (0.17, 0.58); P = 0.002), and box isolation (OR: 0.37, 95% CI (0.20, 0.69); P = 0.002) subgroups, but the type of AF, follow up &gt;12 months and homogenization subgroups were not statistically significant. Trial sequential analysis shows conclusive evidence for the LVAs ablation.ConclusionThis study has shown that LVAs could improve the risk of arrhythmia recurrence in AF patients after conventional ablation. And additional LVAs substrate modification after conventional ablation could increase the freedom of arrhythmia recurrence in LVAs patients. Interestingly, the box isolation approach appeared more promising.Systematic review registration[http://www.crd.york.ac.uk/prospero], identifier [CRD42021239277].
Land ecological protection polices improve ecosystem services: A case study of Lishui, China
Authors: Tang Yisheng , Tang Jinghao , Yu Xianghong , Qiu Lefeng , Wang Jingyi , Hou Xianrui , Chen Dongxiang 
Published: September 2022

Ecological priority and green development have become the main theme of the times in China. The policy performance evaluation of ecological protection needs to quantitatively identify the changes of ecosystem quality and services firstly. This paper constructed the analytical framework driven by ecological protection policy of “Goal-oriented—Policy driven—Ecological quality–Service improvement”, and used multi-source data to establish the evaluation approach of “ecosystem quality-ecosystem services-ecosystem services value”. This study took Lishui as a case study to confirm the framework proposed. The results show that the ecosystem quality of Lishui has been steadily improved in recent 10 years. The overall quality of ecosystem services such as vegetation oxygen release, carbon sequestration, pollution removal, cooling service, humidification regulation and water conservation service has been improved by a range of 2%–6%. The value of ecological products has increased from 143.28 billion CNY in 2009 to 150.23 billion CNY in 2019. Lishui has implemented the development concept of “ecological civilization”, and the policies of ecological restoration or land remediation have changed land use and ecosystem quality, which was the main driving force for the improvement of ecological quality and the main promotion of ecological products value. The methods and results can provide insight into the impact of land policies on ecosystem services and decisions that support for further optimizing land ecological protection policies.
Detection of anti-SARS-CoV-2-Spike/RBD antibodies in vaccinated elderly from residential care facilities in Romania, April 2021
Authors: Vremera Teodora , Furtunescu Florentina Ligia , Leustean Mihaela , Rafila Alexandru , David Adina , Radu Iuliana , Cornienco Ana Maria , Gatea Adina , Ilie Ciprian , Iancu Luminita Smaranda , Pistol Adriana 
Published: September 2022

IntroductionSARS-CoV-2 infection rates and related mortality in elderly from residential care facilities are high. The aim of this study was to explore the immune status after COVID-19 vaccination in people 65 years and older.MethodsThe study involved volunteer participants living in residential care facilities. The level of anti-Spike/RBD antibodies was measured at 2–12 weeks after complete vaccination, using chemiluminescent microparticle immunoassay (SARS-CoV-2 IgG II Quant Abbott).ResultsWe have analyzed 635 serum samples collected from volunteers living in 21 Residential Care Facilities. With one exception, in which the vaccination was done with the Moderna vaccine, all volunteers received the Pfizer-Comirnaty vaccine. Individuals enrolled in the study had ages between 65–110 years (median 79 years). Of the people tested, 54.8% reported at least one comorbidity and 59.2% reported having had COVID-19 before vaccination. The presence of anti-S/RBD antibodies at a protective level was detected in 98.7% of those tested (n = 627 persons) with a wide variation of antibody levels, from 7.1 to 5,680 BAU/ml (median 1287 BAU/ml). Antibody levels appeared to be significantly correlated to previous infection (r = 0.302, p = 0.000).ConclusionsThe study revealed the presence of anti-SARS CoV-2 antibodies in a significant percentage of those tested (98.7%). Of these, more than half had high antibody levels. Pre-vaccination COVID-19 was the only factor found to be associated with higher anti-S/RBD levels. The significant response in elderly people, even in those with comorbidities, supports the vaccination measure for this category, irrespective of associated disabilities or previous infection.
Altered local gyrification and functional connectivity in type 2 diabetes mellitus patients with mild cognitive impairment: A pilot cross-sectional small-scale single center study
Authors: Shao Pengfei , Li Xin , Qin Ruomeng , Xu Hengheng , Sheng Xiaoning , Huang Lili , Ma Junyi , Cheng Yue , Chen Haifeng , Zhang Bing , Zhao Hui , Xu Yun 
Published: September 2022

AimsThis research aimed to explore alterations in the local gyrification index (GI) and resting-state functional connectivity (RSFC) in type 2 diabetes mellitus (T2DM) patients with mild cognitive impairment (MCI).MethodsIn this study, 126 T2DM patients with MCI (T2DM-MCI), 154 T2DM patients with normal cognition (T2DM-NC), and 167 healthy controls (HC) were recruited. All subjects underwent a battery of neuropsychological tests. A multimodal approach combining surface-based morphometry (SBM) and seed-based RSFC was used to determine the structural and functional alterations in patients with T2DM-MCI. The relationships among the GI, RSFC, cognitive ability, and clinical variables were characterized.ResultsCompared with the T2DM-NC group and HC group, T2DM-MCI patients showed significantly reduced GI in the bilateral insular cortex. Decreased RSFC was found between the left insula and right precuneus, and the right superior frontal gyrus (SFG). The altered GI was correlated with T2DM duration, global cognition, and episodic memory. The mediation effects of RSFC on the association between GI and cognition were not statistically significant.ConclusionOur results suggest that GI may serve as a novel neuroimaging biomarker to predict T2DM-related MCI and help us to improve the understanding of the neuropathological effects of T2DM-related MCI.
COVID-19 and the risk of CNS demyelinating diseases: A systematic review
Authors: Lotan Itay , Nishiyama Shuhei , Manzano Giovanna S. , Lydston Melissa , Levy Michael 
Published: September 2022

BackgroundViral infections are a proposed possible cause of inflammatory central nervous system (CNS) demyelinating diseases, including multiple sclerosis (MS), neuromyelitis optica spectrum disorder (NMOSD), and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD). During the past 2 years, CNS demyelinating events associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been reported, but causality is unclear.ObjectiveTo investigate the relationship between CNS demyelinating disease development and exacerbation with antecedent and/or concurrent SARS-CoV-2 infection.MethodsA systematic literature review of all publications describing either a new diagnosis or relapse of CNS demyelinating diseases (MS, NMOSD, MOGAD) in association with SARS-CoV-2 infection was performed utilizing PRISMA guidelines. Descriptive statistics were used for data analysis, using a case analysis approach.ResultsSixty-seven articles met the inclusion criteria for the study. Most of the reported cases of NMOSD (n= 13, 72.2% of reported cases) and MOGAD (n= 27, 96.5% of reported cases) were of new disease onset, presenting with typical clinical and radiographic features of these conditions, respectively. In contrast, reported MS cases varied amongst newly diagnosed cases (n= 10, 10.5% of reported cases), relapses (n= 63, 66.4%) and pseudo-relapses (n= 22, 23.2%). The median duration between COVID-19 infection and demyelinating event onset was 11.5 days (range 0–90 days) in NMOSD, 6 days (range−7 to +45 days) in MOGAD, and 13.5 days (range−21 to +180 days) in MS. Most cases received high-dose corticosteroids with a good clinical outcome.ConclusionBased upon available literature, the rate of CNS demyelinating events occurring in the setting of preceding or concurrent SARS-CoV-2 infection is relatively low considering the prevalence of SARS-CoV-2 infection. The clinical outcomes of new onset or relapsing MS, NMOSD, or MOGAD associated with antecedent or concurrent infection were mostly favorable. Larger prospective epidemiological studies are needed to better delineate the impact of COVID-19 on CNS demyelinating diseases.
Intravenous thrombolysis with 0.65 mg/kg r-tPA may be optimal for Chinese mild-to-moderate stroke
Authors: Cui Yu , Yao Zhi-Guo , Chen Hui-Sheng 
Published: September 2022

BackgroundIntravenous recombinant tissue plasminogen activator (r-tPA) with 0.9 mg/kg is the standard treatment for acute ischemic stroke, but it remains unclear whether it is optimal for all patients. We aimed to determine the optimal dose of r-tPA for Chinese stroke based on the data from the INTRECIS study.MethodsFrom the INTRECIS cohort, patients receiving intravenous r-tPA within 4.5 h of onset were included. According to r-tPA dose, patients were assigned into seven groups (from 0.60 to 0.90 mg/kg). The primary outcomes were the proportion of excellent functional outcomes and symptomatic intracranial hemorrhage.ResultsOverall, 2,666 patients were included: 156 in 0.60 mg/kg group, 117 in 0.65 mg/kg group, 127 in 0.70 mg/kg group, 188 in 0.75 mg/kg group, 154 in 0.80 mg/kg group, 359 in 0.85 mg/kg group, and 1,565 in 0.90 mg/kg group. After adjustment for baseline characteristics, only 0.65 mg/kg group had significantly higher proportion of excellent functional outcome than 0.90 mg/kg group (79.5 vs. 71.4%, odds ratio = 1.833, 95% CI = 1.006–3.341, adjusted p = 0.048). The subgroup analysis showed no evidence of differences in the odds of having a primary outcome between the two groups by age, admission NIHSS, onset to thrombolysis time, and TOAST classification. There was no significant difference in symptomatic intracranial hemorrhage between groups.ConclusionOur study presented the first evidence that intravenous thrombolysis with 0.65 mg/kg r-tPA may be optimal for Chinese mild-to-moderate stroke.Registrationhttps://www.clinicaltrials.gov, identifier: NCT 02854592.
Predictors of response to acetylcholinesterase inhibitors in dementia: A systematic review
Authors: Pozzi Federico Emanuele , Conti Elisa , Appollonio Ildebrando , Ferrarese Carlo , Tremolizzo Lucio 
Published: September 2022

BackgroundThe mainstay of therapy for many neurodegenerative dementias still relies on acetylcholinesterase inhibitors (AChEI); however, there is debate on various aspects of such treatment. A huge body of literature exists on possible predictors of response, but a comprehensive review is lacking. Therefore, our aim is to perform a systematic review of the predictors of response to AChEI in neurodegenerative dementias, providing a categorization and interpretation of the results.MethodsWe conducted a systematic review of the literature up to December 31st, 2021, searching five different databases and registers, including studies on rivastigmine, donepezil, and galantamine, with clearly defined criteria for the diagnosis of dementia and the response to AChEI therapy. Records were identified through the string:predict*AND respon*AND (acetylcholinesterase inhibitors OR donepezil OR rivastigmine OR galantamine). The results were presented narratively.ResultsWe identified 1,994 records in five different databases; after exclusion of duplicates, title and abstract screening, and full-text retrieval, 122 studies were finally included.DiscussionThe studies show high heterogeneity in duration, response definition, drug dosage, and diagnostic criteria. Response to AChEI seems associated with correlates of cholinergic deficit (hallucinations, fluctuating cognition, substantia innominate atrophy) and preserved cholinergic neurons (faster alpha on REM sleep EEG, increased anterior frontal and parietal lobe perfusion after donepezil); white matter hyperintensities in the cholinergic pathways have shown inconsistent results. The K-variant of butyrylcholinesterase may correlate with better response in late stages of disease, while the role of polymorphisms in other genes involved in the cholinergic system is controversial. Factors related to drug availability may influence response; in particular, low serum albumin (for donepezil), CYP2D6 variants associated with reduced enzymatic activity and higher drug doses are the most consistent predictors, while AChEI concentration influence on clinical outcomes is debatable. Other predictors of response include faster disease progression, lower serum cholesterol, preserved medial temporal lobes, apathy, absence of concomitant diseases, and absence of antipsychotics. Short-term response may predict subsequent cognitive response, while higher education might correlate with short-term good response (months), and long-term poor response (years). Age, gender, baseline cognitive and functional levels, and APOE relationship with treatment outcome is controversial.
Comprehensive analysis of m6A-modified circRNAs in peritoneal metastasis of high grade serious carcinoma of ovary
Authors: Guo Lin , Xu Nini , Qiu Daner , Yang Xiaozhe , Zhao Shasha , Zhao Hongxi 
Published: September 2022

PurposeHigh-grade serous ovarian cancer (HGSOC) remains the most lethal female cancer due to metastasis. CircRNAs are recently identified to be modified by N6-methyladenosine (m6A) in many cells. However, the significance of m6A-modified circular RNAs (circRNAs) has not been elucidated in HGSOC peritoneal metastasis. Here, we aimed to investigate the participation and potential functions of m6A-modified circRNAs in HGSCO peritoneal metastasis.MethodsCancerous tissues were collected from the in situ and the peritoneal metastasis lesions of HGSCO patients. M6A-tagged circRNAs were identified by m6A-modified RNA immunoprecipitation sequencing (m6A-RIP-seq). Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the potential functions of the m6A-modified circRNAs.ResultsFor the m6A-modified circRNAs, 259 were upregulated and 227 were downregulated in the peritoneal metastasis than in the situ lesions of HGSCO patients. For the m6A peaks, 1541 were upregulated and 1293 were downregulated in the peritoneal metastasis than in the in situ lesions of HGSCO patients. For the differential expressed circRNAs, 1911(19.6%) were upregulated and 2883(29.6%) were downregulated in the peritoneal metastasis than in the in situ lesions of HGSCO patients. The upregulated m6A-modified circRNAs were associated with the HIF-1 signaling. The downregulated m6A-modified circRNAs were associated with the MAPK signaling.ConclusionsThis work firstly identified the transcriptome-wide map of m6A-modified circRNAs in peritoneal metastasis of HGSCO. Our findings provided novel evidences about the participation of m6A-modified circRNAs via HIF-1 and MAPK signaling and a new insight in molecular target of HGSCO peritoneal metastasis.