bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recurrent Respiratory Papillomatosis: Quality of Life Data from an International Patient Registry
Authors: So Raymond J. , McClellan Kim , Best Simon R. 
Published: September 2022

ObjectivesQuality of life studies in recurrent respiratory papillomatosis (RRP) have traditionally relied upon clinician‐designed survey instruments. This study's objective is to report quality of life outcomes from a patient‐designed questionnaire.MethodsPatients who provided health information and completed a quality of life questionnaire were identified from the RRPF‐CoRDS patient registry. Demographic, clinical, and quality of life measures were collected. Means and standard deviations were calculated for continuous data, and frequencies and percentages were calculated for categorical data. Outcomes for patients with juvenile‐onset RRP (JORRP) and adult‐onset RRP (AORRP) were compared using Student's t‐tests for continuous data and χ2 analyses for categorical data.ResultsSeventy‐three patients with RRP were identified (JORRP: 32; AORRP: 41). Common clinical symptoms included raspy voice (78.1%) and dyspnea (61.6%). The majority (97.3%) of patients reported feeling debilitated by their diagnosis, and 94.5% of patients reported avoiding participation in career and/or social activities due to their voice quality. Due to their RRP, 65.7% reported missing at least five work days each month. Social anxiety was reported in 79.5% of patients, though only 28.8% of the cohort reported utilizing mental health services. The median (range) lifetime number of surgeries received was 20 (1 ‐ 300). Most patients (57.5%) reported paying at least 5% of their annual income towards RRP‐related medical care.ConclusionRRP presents high mental and fiscal burden. Our results highlight data from a quality of life questionnaire designed by RRP patients, and may help to elucidate potential disconnects between what clinicians and RRP patients consider most impactful.Level of Evidence4 Laryngoscope, 133:1919–1926, 2023
Recent Advances in 3D Printing of Photocurable Polymers: Types, Mechanism, and Tissue Engineering Application
Authors: Randhawa Aayushi , Dutta Sayan Deb , Ganguly Keya , Patel Dinesh K. , Patil Tejal V. , Lim Ki‐Taek 
Published: September 2022

AbstractThe conversion of liquid resin into solid structures upon exposure to light of a specific wavelength is known as photopolymerization. In recent years, photopolymerization‐based 3D printing has gained enormous attention for constructing complex tissue‐specific constructs. Due to the economic and environmental benefits of the biopolymers employed, photo‐curable 3D printing is considered an alternative method for replacing damaged tissues. However, the lack of suitable bio‐based photopolymers, their characterization, effective crosslinking strategies, and optimal printing conditions are hindering the extensive application of 3D printed materials in the global market. This review highlights the present status of various photopolymers, their synthesis, and their optimization parameters for biomedical applications. Moreover, a glimpse of various photopolymerization techniques currently employed for 3D printing is also discussed. Furthermore, various naturally derived nanomaterials reinforced polymerization and their influence on printability and shape fidelity are also reviewed. Finally, the ultimate use of those photopolymerized hydrogel scaffolds in tissue engineering is also discussed. Taken together, it is believed that photopolymerized 3D printing has a great future, whereas conventional 3D printing requires considerable sophistication, and this review can provide readers with a comprehensive approach to developing light‐mediated 3D printing for tissue‐engineering applications.
Process Dependent Complexity in Multicomponent Gels
Authors: Randle Rebecca I. , Ginesi Rebecca E. , Matsarskaia Olga , Schweins Ralf , Draper Emily R. 
Published: September 2022

AbstractMixing low molecular weight gelators (LMWGs) can be used to combine favorable properties of the individual components within a multifunctional gel. Such multicomponent systems are complex enough in themselves but the method of combining components is not commonly considered something to influence self‐assembly. Herein, two multicomponent systems comprising of a naphthalene‐based dipeptide hydrogelator and one of two modified naphthalene diimides (NDIs), one of which forms gels, and the other does not, are investigated. These systems are probed, examining the structures formed and their gel properties (when preparing a solution from either a mixed powder of both components or by mixing pre‐formed solutions of each component) using rheology, small angle neutron scattering (SANS), and absorbance spectroscopy. It is found that by altering the method of preparation, it is can either induce self‐sorting or co‐assembly within the fibers formed that underpin the gel network.
Systematic metabolic characterization of mental disorders reveals age‐related metabolic disturbances as potential risk factors for depression in older adults
Authors: Liu Yu , Zhao Wanyu , Lu Ying , Zhao Yunli , Zhang Yan , Dai Miao , Hai Shan , Ge Ning , Zhang Shuting , Huang Mingjin , Liu Xiaohui , Li Shuangqing , Yue Jirong , Lei Peng , Dong Biao , Dai Lunzhi , Dong Birong 
Published: September 2022

AbstractMental disorders are associated with dysregulated metabolism, but comprehensive investigations of their metabolic similarities and differences and their clinical relevance are few. Here, based on the plasma metabolome and lipidome of subcohort1, comprising 100 healthy participants, 55 cases with anxiety, 52 persons with depression, and 41 individuals with comorbidity, which are from WCHAT, a perspective cohort study of community‐dwelling older adults aged over 50, multiple metabolites as potential risk factors of mental disorders were identified. Furthermore, participants with mental illnesses were classified into three subtypes (S1, S2, and S3) by unsupervised classification with lipidomic data. Among them, S1 showed higher triacylglycerol and lower sphingomyelin, while S2 displayed opposite features. The metabolic profile of S3 was like that of the normal group. Compared with S3, individuals in S1 and S2 had worse quality of life, and suffered more from sleep and cognitive disorders. Notably, an assessment of 6,467 individuals from the WCHAT showed an age‐related increase in the incidence of depression. Seventeen depression‐related metabolites were significantly correlated with age, which were validated in an independent subcohort2. Collectively, this work highlights the clinical relevance of metabolic perturbation in mental disorders, and age‐related metabolic disturbances may be a bridge‐linking aging and depressive.
Mendelian Randomization Study Using Dopaminergic Neuron‐Specific eQTL Nominates Potential Causal Genes for Parkinson's Disease
Authors: Dang Xinglun , Zhang Zhijun , Luo Xiong‐Jian 
Published: September 2022

AbstractBackgroundLarge‐scale genome‐wide association studies (GWASs) have reported multiple risk variants for Parkinson's disease (PD). However, little is known about how these reported risk variants confer risk of PD.ObjectiveTo nominate genes whose genetically regulated expression in dopaminergic neurons may have a causal role in PD.MethodsWe conducted a two‐sample Mendelian randomization (MR) study by integrating large‐scale genome‐wide associations and expression quantitative trait loci (eQTL) data from dopaminergic neurons.ResultsMR analysis nominated 10 risk genes whose genetically regulated expression in dopaminergic neurons may have a causal role in PD. These MR significant genes include FAM200B, NDUFAF2, NUP42, SH3GL2, STX1B, CCDC189, KAT8, PRSS36, VAMP4, and ZSWIM7.ConclusionsWe report the first MR study of PD by using dopaminergic neuron‐specific eQTL and nominate novel risk genes for PD. Further functional characterization of the nominated risk genes will provide mechanistic insights into PD pathogenesis and potential therapeutic targets. © 2022 International Parkinson and Movement Disorder Society.
Mutual leveraging of proximity effects and click chemistry in chemical biology
Authors: Dong Ru , Yang Xiaoxiao , Wang Binghe , Ji Xingyue 
Published: September 2022

AbstractNature has the remarkable ability to realize reactions under physiological conditions that normally would require high temperature and other forcing conditions. In doing so, often proximity effects such as simultaneous binding of two reactants in the same pocket and/or strategic positioning of catalytic functional groups are used as ways to achieve otherwise kinetically challenging reactions. Though true biomimicry is challenging, there have been many beautiful examples of how to leverage proximity effects in realizing reactions that otherwise would not readily happen under near‐physiological conditions. Along this line, click chemistry is often used to endow proximity effects, and proximity effects are also used to further leverage the facile and bioorthogonal nature of click chemistry. This review brings otherwise seemingly unrelated topics in chemical biology and drug discovery under one unifying theme of mutual leveraging of proximity effects and click chemistry and aims to critically analyze the biomimicry use of such leveraging effects as powerful approaches in chemical biology and drug discovery. We hope that this review demonstrates the power of employing mutual leveraging proximity effects and click chemistry and inspires the development of new strategies that will address unmet needs in chemistry and biology.
Consensus Virtual Screening Protocol Towards the Identification of Small Molecules Interacting with the Colchicine Binding Site of the Tubulin‐microtubule System
Authors: López‐López Edgar , Cerda‐García‐Rojas Carlos M. , Medina‐Franco José L. 
Published: September 2022

AbstractModification of the tubulin‐microtubule (Tub‐Mts) system has generated effective strategies for developing different treatments for cancer. A huge amount of clinical data about inhibitors of the tubulin‐microtubule system have supported and validated the studies on this pharmacological target. However, many tubulin‐microtubule inhibitors have been developed from representative and common scaffolds that cover a small region of the chemical space with limited structural innovation. The main goal of this study is to develop the first consensus virtual screening protocol for natural products (ligand‐ and structure‐based drug design methods) tuned for the identification of new potential inhibitors of the Tub‐Mts system. A combined strategy that involves molecular similarity, molecular docking, pharmacophore modeling, and in silico ADMET prediction has been employed to prioritize the selections of potential inhibitors of the Tub‐Mts system. Five compounds were selected and further studied using molecular dynamics and binding energy predictions to characterize their possible binding mechanisms. Their structures correspond to 5‐[2‐(4‐hydroxy‐3‐methoxyphenyl) ethyl]‐2,3‐dimethoxyphenol (1), 9,10‐dihydro‐3,4‐dimethoxy‐2,7‐phenanthrenediol (2), 2‐(3,4‐dimethoxyphenyl)‐5,7‐dihydroxy‐6‐methoxy‐4H‐1‐benzopyran‐4‐one (3), 13,14‐epoxyparvifoline‐4’,5’,6’‐trimethoxybenzoate (4), and phenylmethyl 6‐hydroxy‐2,3‐dimethoxybenzoate (5). Compounds 1–3 have been associated with literature reports that confirm their activity against several cancer cell lines, thus supporting the utility of this protocol.
IM‐MS in der Bioanalytik: Isomere unterscheiden in der Gasphase
Authors: Geue Niklas , Barran Perdita 
Published: September 2022

AbstractMassenspektrometrie kombiniert mit Ionenmobilitäts‐Massenspektrometrie (IM‐MS) liefert im selben Experiment Daten zur Masse und Struktur von Probenmolekülen. Ein wichtiges Anwendungsfeld ist Identifizierung und Trennung von Isomeren, beispielsweise von Oligosacchariden und Proteinen.
Bioethanol: Zügig gegärt
Authors: Sietz Manfred , Siemens Yvonne 
Published: September 2022

AbstractEin kristalliner Oxidationskatalysator aus Nanogold auf Kieselgel beschleunigt die alkoholische Gärung von Zucker‐ und Dicksaftlösungen. Katalytisch wirkt unter anderen das gärgeschwindigkeitsbestimmende Coenzym Nicotinamidadenindinucleotid.
Recycling: Vom Schlamm zum Granulat
Authors: Moebius Heinzhorst , Schmidt Horst , Hippmann Sebastian 
Published: September 2022

AbstractDer Bauxitrückstand aus der Aluminiumproduktion lässt sich wiederverwenden, statt ihn als Rotschlamm zu deponieren. Wie aus Abfall ein Wertstoff wird.
A transition programme to enhance ICU new graduate nurses' professional identity and intention to remain employed: A pre‐ and postevaluation
Authors: Hu Fen , Ding Xin‐Bo , Zhang Ru‐Han , Li Su‐Yun , Cao Rui , Deng Lan , Li Jin , Fu Xing , Zhu Xiao‐Ping , Chen Jie 
Published: September 2022

AbstractAimsTo investigate the effect of a transition programme including cognitive‐behaviour‐based preceptorship intervention on ICU new graduate nurses' (NGNs)' professional identity and their intention to remain employed.DesignOne‐arm pre‐ and post‐test design followed the TREND Statement.MethodsNGNs' professional identity and intention to remain employed were measured at baseline (T1), postintervention (1 month, T2) and 6‐month follow‐up (T3).ResultsFifty‐three NGNs completed the programme, and their professional identity score significantly increased postintervention [from 111.5 (SD = 16.72) at T1 to 114.2 (SD = 9.12) at T2, p < 0.05], but decreased at T3 [105.8 (SD = 9.44) compared with T1 and T2, p < 0.05]. There was no significant difference in ICU NGNs' intention of remain employed outcomes at three time points (p > 0.05). The transition programme was effective to improve ICU NGNs' professional identity immediately after the intervention, but the increment did not maintain at the 6‐month follow‐up.
A structure‐preserving finite element approximation of surface diffusion for curve networks and surface clusters
Authors: Bao Weizhu , Garcke Harald , Nürnberg Robert , Zhao Quan 
Published: September 2022

AbstractWe consider the evolution of curve networks in two dimensions (2d) and surface clusters in three dimensions (3d). The motion of the interfaces is described by surface diffusion, with boundary conditions at the triple junction points lines, where three interfaces meet, and at the boundary points lines, where an interface meets a fixed planar boundary. We propose a parametric finite element method based on a suitable variational formulation. The constructed method is semi‐implicit and can be shown to satisfy the volume conservation of each enclosed bubble and the unconditional energy‐stability, thus preserving the two fundamental geometric structures of the flow. Besides, the method has very good properties with respect to the distribution of mesh points, thus no mesh smoothing or regularization technique is required. A generalization of the introduced scheme to the case of anisotropic surface energies and non‐neutral external boundaries is also considered. Numerical results are presented for the evolution of two‐dimensional curve networks and three‐dimensional surface clusters in the cases of both isotropic and anisotropic surface energies.
Chromosomal microarray analysis in pregnancy loss: Is it time for a consensus approach?
Authors: Schilit Samantha L. P. , Studwell Courtney , Flatley Pamela , Listewnik Marc , Mertens Lauren , Ligon Azra H. , Mason‐Suares Heather 
Published: September 2022

AbstractObjectiveTo investigate the efficacy and outcomes of chromosomal microarray (CMA) in the cytogenomic evaluation of products of conception (POC).MethodOver a 42‐month period, 323 POC samples were tested by CMA. Results were assessed using variables including phenotype, gestational age, results from orthogonal testing, and follow‐up parental analysis.ResultsCMA identified cytogenetic abnormalities in 47.4% of first trimester losses and 10.9% of second and third trimester losses. Chromosomal microarray results specifically from 5 to 7‐week losses showed similar rates of abnormalities (45.6%) compared to those of all first trimester losses combined. CMA and karyotype results were discordant in 20.0% of cases, most likely due to maternal cell overgrowth in culture. The most prevalent abnormalities identified in all losses were autosomal trisomies, followed by triploidy. In 43/323 cases, the observed abnormality suggested a parental aberration that prompted follow‐up studies; two of these cases indeed identified an inherited aberration.ConclusionOur findings of specific types of genetic abnormalities and the respective frequencies by gestational age closely align with those of published karyotype studies, supporting the use of routine CMA testing for POCs. CMA outperforms karyotype analysis because it does not require viable, sterile cultures free of maternal admixture or admixture due to multiple gestations. Finally, CMA results can play an important role in identifying increased recurrence risks for some couples.
Development of functional anti‐Gn nanobodies specific for SFTSV based on next‐generation sequencing and proteomics
Authors: Wang Binghao , Huang Bilian , Li Xinyu , Guo Yan , Qi Guantong , Ding Yibing , Gao Haidong , Zhang Jingzi , Wu Xilin , Fang Lei 
Published: September 2022

AbstractSevere fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by novel bunyavirus (SFTSV), with a mortality rate of 6.3% ~ 30%. To date, there is no specific treatment for SFTS. Previously, we demonstrated that SFTSV surface glycoprotein (Glycoprotein N, Gn) was a potential target for the development of SFTS vaccine or therapeutic antibodies, and anti‐Gn neutralizing antibodies played a protective role in SFTS infection. Compared with traditional antibodies, nanobodies from camelids have various advantages, including small molecular weight, high affinity, low immunogenicity, convenient production by gene engineering, etc. In this study, we combined next‐generation sequencing (NGS) with proteomics technology based on affinity purification‐mass spectrometry (AP‐MS) and bioinformatics analysis to high‐throughput screen monoclonal anti‐Gn nanobodies from camel immunized with Gn protein. We identified 19 anti‐Gn monoclonal nanobody sequences, of which six sequences were selected for recombinant protein expression and purification. Among these six anti‐Gn nanobodies, nanobody 57,493 was validated to be highly specific for Gn. The innovative high‐throughput technical route developed in this study could also be expanded to the production of nanobodies specific for other viruses like SARS‐CoV‐2.
Group housing enhances mating and increases the sensitization of chemical cues in Bactrocera dorsalis
Authors: Chen Yaoyao , Zhang Yuhua , Yang Liying , Chen Wenlong , Jiang Zhiyan , Xiao Ziwei , Xie Xin , Zhong Guohua , Yi Xin 
Published: September 2022

AbstractBackgroundChanges in population density have profound impacts on mating behaviors in group‐living animals. The plasticity of mating behavior enables insects to respond to social signals and adjust mating frequency in accordance with rival competition and reproductive opportunity.ResultsIn this study, we found that low levels of cis‐vaccenyl acetate (cVA), a Drosophila pheromone, increased mating rates of Bactrocera dorsalis, but high concentrations of cVA inhibited mating, indicating a functional role of cVA in regulating mating behaviors in insect species other than Drosophila. Moreover, we demonstrated that group housing conditions had positive effects for B. dorsalis on their mating rates, responses toward cVA and cVA‐mediated mating behaviors, which are dependent on the activity of c‐AMP reponse element binding protein (CREB) binding protein (CBP).ConclusionsOur data suggest that CBP‐mediated plasticity in mating behavior and chemical recognition enables insects to adapt to different housing conditions and highlight the potential of cVA as an efficient agent in regulating mating behaviors in insect species other than Drosophila. © 2022 Society of Chemical Industry.
The odorant receptor co‐receptor gene contributes to mating and host‐searching behaviors in parasitoid wasps
Authors: Zhang Qichao , Chen Jiani , Wang Ying , Lu Yueqi , Dong Zhi , Shi Wenqi , Pang Lan , Ren Shaopeng , Chen Xuexin , Huang Jianhua 
Published: September 2022

AbstractBackgroundBiological control of pest insects by parasitoid wasps is an effective and environmentally friendly strategy compared with the use of synthetic pesticides. Successful courtship and host‐search behaviors of parasitoid wasps are important for biological control efficiency and are often mediated by chemical odorant cues. The odorant receptor co‐receptor (Orco) gene has an essential role in the perception of odors in insects. However, the function of Orco in the mating and host‐searching behaviors of parasitoid wasps remains underexplored.ResultsWe identified the full‐length Orco genes of four Drosophila parasitoid species in the genus Leptopilina, namely L. heterotoma, L. boulardi, L. syphax and L. drosophilae. Sequence alignment and membrane‐topology analysis showed that Leptopilina Orcos had similar amino acid sequences and topology structures. Phylogenetic analysis revealed that Leptopilina Orcos were highly conserved. Furthermore, the results of quantitative real‐time polymerase chain reactions showed that all four Orco genes had a typical antennae‐biased tissue expression pattern. After knockdown of Orco in these different parasitoid species, we found that Orco‐deficient male parasitoid wasps, but not females, lost their courtship ability. Moreover, Orco‐deficient female parasitoid wasps presented impaired host‐searching performance and decreased oviposition rates.ConclusionOur study demonstrates that Orcos are essential in the mating and host‐searching behaviors of parasitoid wasps. To our knowledge, this is the first time that the functions of Orco genes have been characterized in parasitoid wasps, which broadens our understanding of the chemoreception basis of parasitoid wasps and contributes to developing advanced pest management strategies. © 2022 Society of Chemical Industry.
The Macklin effect in tension pneumomediastinum in a patient with interstitial pneumonia
Authors: Watanabe Hiroko , Ishikawa Hiroaki , Inui Toshihide , Kawashima Kai , Namiki Tomohiro , Sakamoto Tohru 
Published: September 2022

Key messageTension pneumomediastinum is a rare complication of interstitial pneumonia. This case shows computed tomography findings of the Macklin effect, in which air dissection along the bronchovascular interstitium caused by alveolar rupture leads to pneumomediastinum.
Optimisation and validation of a co‐manipulated robot for brachytherapy procedure
Authors: Ben Halima Aziza , Bert Julien , Clément Jean‐François , Visvikis Dimitris 
Published: September 2022

AbstractBackgroundLow‐dose rate brachytherapy is the referent treatment for early‐stage prostate cancer and consists in manually inserting radioactive seeds within the organ to destroy tumorous cells. This treatment is inaccurate leading to side effects. Researchers developed robots to improve this technique. Despite ameliorating accuracy, they cannot be clinically used because of size and acceptability. Therefore, a 6‐DOF parallel and co‐manipulated robot is proposed to meet these requirements.MethodsTo fulfil the application requirements, a compact design was modelled. The robot's optimal dimensions were defined by establishing kinematics and implementing genetic algorithm. The robot's relevance was evaluated by measuring workspace and needle placement errors.ResultsThe robot fits into a cube of 300 × 300 × 300 mm3 and provides a free‐singularity workspace of 55 × 55 × 150 mm3 with a possible end‐effector rotation of 15° and a needle placement error <3 mm.ConclusionThe results are promising and prove that our robot fulfils the application requirements and presents a beneficial alternative to the manual procedure.