bluuebunny/crossref_metadata_2025_split huggingface dataset:
Native T1 and T2 Reference Values for Maltese Healthy Cohort
Authors: Yamagata Kentaro , Yamagata Lara Marie , Abela Mark , Mifsud Claude Portanier , Micallef Lee Ann , Reichmuth Luise , Borg Alexander 
Published: June 2022

Abstract
BackgroundCardiac Magnetic Resonance (CMR) is increasingly being used for diagnosing various cardiac conditions. Parametric mapping enables quantitative myocardial characterization by directly measuring myocardial T1 and T2 values. However, reference values of parametric mapping are not standardized across different vendors and scanners, causing drawbacks for clinical implementation of this technique across different sites. We assessed the reference ranges of native T1 and T2 values in a healthy Maltese cohort to establish a local parametric mapping service. Methods and ResultsHealthy subjects (n=51; mean age 36.0 [range 19-59] years) with normal cardiac function on CMR were recruited. Subjects underwent uniform parametric mapping pulse sequences (MOLLI 5(3)3 for native T1 mapping, and gradient echo single shot FLASH readout for T2 mapping) on a 3T Siemens MAGNETOM Vida scanner. Native T1 and T2 values were measured by placing a region of interest within the interventricular septum at midventricular level. Intra- and inter-observer variability were assessed using Bland-Altman plots. Normal distribution of data was assessed using Kolmogorov-Smirnov test (p-value of 0.773 for native T1, p-value of 0.125 for T2). Mean ±1.96 SD was used as a reference range. Mean native T1 and T2 values were 1200.1 ±30.7 ms and 39.5 ±1.8 ms, respectively. All parameters had excellent day-to-day intra-observer reproducibility and inter-observer reproducibility. ConclusionFor the first time in Malta, we established the native T1 and T2 parametric mapping reference values for healthy Caucasian Maltese individuals. This will assist cardiologists to establish diagnosis, disease progression, and response to treatment of various myocardial diseases locally.
Effects of ACTH Treatment in Infantile Spasm; Insights from Electrocardiography and Echocardiography
Authors: Cevik berna saylan , ünver olcay , Doğancı demet deniz , Sağer Güneş , akalın figen , türkdoğan dilşad 
Published: June 2022

Abstract
Objective
Infantile spasms (ISs) are a group of epileptic disorders, which represents the most frequent type of epilepsy in the first year of life. The most effective treatment for IS is adrenocorticotropic hormone (ACTH). We hypothesized that ACTH treatment might have cardiac adverse effects. This study aimed to evaluate the effects of ACTH treatment on the heart muscle and/or arrhythmia risk in patients with IS.
Methods
Twenty-four patients with IS and 24 healthy children (control group) participated in the study. Electroencephalography (EEG), electrocardiography (ECG), and transthoracic echocardiography were studied during treatment, and evaluations were made at 0-2-4 and 6th months of the treatment.
Results
None of the patients had significant tachycardia or bradycardia when compared with the healthy group, or during treatment. On the other hand, values of the PR, QRS, QT, tp/te, tpte/QT, and tpte/QTc values were significantly increased compared with either pretreatment values or each other, which indicated an increased arrhythmia risk in the patient group. In transthoracic echocardiographic examinations, we observed diminished diastolic dysfunctions, either with pulse wave or TDI Doppler. We found a significant difference between the groups in terms of longitudinal strain parameters.
Conclusion
Although we use low-dose and short-duration ACTH treatment in patients with IS, it may cause subclinical damage to the heart muscle and an increased risk of arrhythmia.
Growth mechanism of nano-plates structured SnS films on different substrates in glancing angle deposition method
Authors: Sazideh Mohammad Reza , Ehsani Mohammad Hosein , Shahidi Mohammad Mahdi , Dizaji H. Rezagholipour 
Published: June 2022

Abstract
In this work, Tin (II) sulfide films have been deposited on glass, Indium Tin Oxide, and Fluorinated Tin Oxide substrates at the deposition angles of 0, 65, and 85 degrees using Physical Vapor Deposition method equipped with Glancing Angle Deposition technique. Based on the results obtained from the X-ray diffraction technique, the crystalline structure of substrates and the angle of depositions along with their effects on the structure of SnS nano-plates have been investigated. Using Raman analysis, the phonons lifetime of the samples was found to change with the type of substrate and the employed deposition angle. Based EDAX analysis, the atomic ratio of Sn to S was observed to change with the change of deposition angle, substrate type and variation the diameter of nano-plates. This phenomenon resulted the formation of the second phase of Sn2S3 which was confirmed by Raman and XRD patterns. The nano-sheets-like growth of all the samples has been confirmed using Felid Emission Scanning Electron Microscopy analysis. For further morphological studies, the Atomic Force Microscopy analysis has been applied, by which the direct relation between the substrate roughness and the final structure of the samples has been observed. The relation between the substrate roughness and the deposition angle in the growth process of SnS nano-sheets has been explained.
Relationship between up-regulation of DTYMK expression and immune infiltration for the prognosis of lung adenocarcinoma
Authors: Zhou Lihua , Zhao Chenqu , Li Jun , Zhang Danni , Lu Biyun , Chen Zongyu , Lian Siyu , Shen Yi , Zhou Leilei , Zhang Xianming 
Published: June 2022

Abstract
BackgroundDeoxythymidylate kinase (DTYMK) is a rate-limiting enzyme in pyrimidine metabolism. Crucially, it is overexpressed in a variety of tumors and is associated with poor prognosis in liver cancer. The role of DTYMK in lung adenocarcinoma remains poorly understood, particularly with respect to immune infiltration. MethodsThe present study aimed to compare the expression of DTYMK between normal tissues, lung adenocarcinoma, and other cancer types using data obtained via The Cancer Genome Atlas, Tumor Immune Estimation Resource 2.0, Gene Expression Database of Normal and Tumor Tissues 2, and Gene Expression Profiling Interactive Analysis (GEPIA). The prognostic value of DTYMK in patients with lung adenocarcinoma was evaluated using GEPIA and Kaplan–Meier plotter. STRING and GeneMANIA were employed to assess protein and gene interaction with DTYMK. R software and TISIDB were used to analyze the correlation between DTYMK expression and immune infiltration. Finally, we validated DTYMK protein expression in lung adenocarcinoma using the UALCAN and HPA databases. ResultsDTYMK expression was significantly increased in various cancer and lung adenocarcinoma tissues (p < 0.05), and was associated with poor prognosis (p < 0.05) in patients with lung adenocarcinoma. In addition, DTYMK was negatively associated with the vast majority of immune cells in lung adenocarcinoma.ConclusionsIncreased expression of DTYMK is associated with poor prognosis in lung adenocarcinoma patients. DTYMK may serve as a potential prognostic biomarker in lung adenocarcinoma. In addition, DTYMK may influence the progression and prognosis of lung adenocarcinoma by influencing the immune microenvironment of tumors.
The “weekday effect” – does it impact esophageal cancer surgery outcomes?
Authors: Jiwnani Sabita , Pramesh CS , Ranganathan Priya 
Published: June 2022

Abstract
Background: Increased 30-day mortality rates have been reported in patients undergoing elective surgery later compared with earlier in the week. However, these reports have been conflicting for esophageal surgery. We conducted a study to assess the differences in outcomes of patients undergoing surgery for esophageal cancer earlier in the week (Tuesday) versus later (Friday). Methods: This retrospective analysis of a prospectively maintained database included patients with esophageal cancer who underwent esophageal resection in a tertiary cancer centre between 1st January 2005 and 31st December 2017. We compared patients operated on Tuesdays versus Fridays. The primary outcome was a composite of major morbidity (defined as Clavien-Dindo grade 3 or more) and/or mortality. Secondary outcomes included duration of post-operative ventilation, and length of ICU and hospital stay. Results: Among 1300 patients included, 733 were operated on Tuesday and 567 on Friday. Patient and surgery characteristics were similar in the two groups. The primary outcome (composite of major morbidity and mortality) was 23.6% in the Tuesday group versus 26.3% in the Friday group. Mortality was similar in the two groups (6.0%). Multivariable logistic regression analysis showed that the day of surgery was not a predictor of major morbidity or mortality.Conclusions: In patients undergoing esophagectomy at tertiary care high volume cancer centre, there was no difference in major morbidity and mortality whether the surgery was performed early in the week(Tuesday) or closer to the weekend(Friday).
Isolation, Screening and Characterisation of Bacillus paramycoides for producing Cellulases, Proteases and Amylases from Fabric Industry
Authors: Sarwan Jyoti , K Jagadeesh Chandra Bose 
Published: June 2022

Abstract
There is availability of numerous of microorganisms with their broad applications in environment and removal of contaminants. World is full of microbes that can produce various enzymes at a time. Bacillus is one of the major species among them. Plant fibres contain complex cellulose and amylopectin and pectin in their structures. For breakdown of these complex molecules like cellulose, amylopectin in simpler molecules like hemicellulose and glucose the enzyme required called cellulases with hydrolytic reactions. Article is based upon isolation, screening, identification and crude enzyme production of isolates from fabric industry.
Patient stories as a knowledge translation strategy for the sustainability of evidence-based interventions (EBIs) in healthcare: A qualitative secondary analysis
Authors: Flynn Rachel , Dobson Lauren , Milne Ella , Campbell Alyson , Mrklas Kelly , Wasylak Tracy , Scott Shannon 
Published: June 2022

Abstract
Background
Patient stories are a knowledge translation (KT) strategy that embody the patient experience. Patient stories are used to inform healthcare policy, delivery, and patient engagement, and have many positive impacts on the uptake of evidence-based interventions (EBIs) in healthcare. However, there is little evidence on the use and influence of patient stories for the long-term sustainability of EBIs. This study aims to understand how patient stories influenced the sustainability of two scaled EBIs in healthcare in the context of Alberta Health Services, Canada.
Methods
We conducted a secondary analysis of 20 qualitative interviews from the realist evaluation: Contextual factors and mechanisms that influence sustainability: A realist evaluation of two scaled, multi-component interventions. We employed a thematic analysis of the interview data.
Results
Patient stories positively influenced the sustainability of two EBIs by: 1) conveying the “why” behind the intervention, 2) motivating and encouraging staff to continue to engage with the intervention long-term, and 3) facilitating the spread and scale of the EBI. Success stories from patients and their families were especially impactful because they reflected positive outcomes of the EBI. Participants emphasized that patient stories should be positive in nature and be continually shared through different modalities (e.g., digital stories) for sustainability efforts.
Conclusions
The idea of patient stories as a KT strategy for sustainability is still nascent and requires more effectiveness and evaluation research. Our findings demonstrate how patient stories can facilitate the sustainability of EBIs in healthcare. In particular, our findings show how sharing stories digitally or through learning collaboratives and online toolkits, facilitates sustainability because digital stories can be saved and continually distributed to a wide audience at any time. Understanding the potential of patient stories as a KT strategy for sustainability will contribute to the long-term success of EBIs, thus improving healthcare quality.
Impact of Simulation Debriefing Structure on Knowledge and Skill Acquisition for Postgraduate Critical Care Nursing Students: three-Phase vs. Multiphase
Authors: Guerrero Jefferson Garcia , Tungpalan-Castro Grace Medalyn , Pingue-Raguini Minerva 
Published: June 2022

Abstract
Background: Simulation is part of the training provided to nurses enrolled in master’s degree for the critical care nursing programs in our institution. Although these are practicing nurses, many still make mistakes when performing nursing procedures related to critical care during simulation sessions, which must be addressed during the debriefing session. The aim of the study is to compare the knowledge and skills acquired by groups of postgraduate critical care nursing students who were exposed to high-fidelity simulation (HFS), by using different structures of debriefingMethods: A quasi-experimental research design was utilized during the post-tests and objective structured clinical examinations (OSCEs). The students were divided into two groups: one was exposed to HFS with a 3-phase debriefing, and the other exposed to HFS with a multiphase debriefing. Both groups involved facilitator-guided and video-assisted debriefings. Results: Overall, the post-test scores (p-value: phase 1 = .001 and phase 2 = .000) and post-OSCE scores (p-value: phase 1 = .002 and phase 2 = .002) support that the group of postgraduate students who underwent HFS with a multiphase debriefing structure gained significantly higher scores compared to the group who underwent HFS with a 3-phase debriefing structure.Conclusion: Debriefing is a critical component to successful simulation. Learning requires assessment that creates constructive criticism based on feedback and reflection. A multiphase debriefing structure, specifically the healthcare simulation after action review (AAR), provides a significant advantage for knowledge and skill acquisition.
Integration of Bulk and Single-Cell RNA-Seq Data to Construct a Prognostic Model of Membrane Tension Related Genes for Colon Cancer
Authors: Li Yong , Li Jiacheng , Fu Yugang , Zhang Kehui , Zhu Yingying , Zhe Shi , Cao Mengxing 
Published: June 2022

Abstract
Background: Plasma membrane provides a highly dynamic barriers for cancer cells to interact with their surrounding microenvironment. Membrane tension, a pivotal physical property of plasma membrane, has attracted more and more attention since it plays a role in the progression of various cancers. However, membrane tension related genes (MTRGs) involved in the regulation of colon cancer are not thoroughly understood. This study aimed to identify a prognostic MTRG signature in colon cancer and explore its implications for the disease.Methods: Bulk and Single-cell RNA-seq data and relevant clinical information were obtained from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database. 44 membrane tension related genes (MTRGs) were obtained through literature. Analysis of differential expressed MTRGs was performed on the RNA-Seq data. By implementing a univariate Cox regression and a LASSO-Cox regression, we developed a prognostic model based on 4 MTRGs. We evaluated the prognostic efficacy of this model using Kaplan–Meier survival curve analysis and receiver operating characteristic (ROC) curve analysis. Moreover, the relationships between this signature and immune cell infiltration, immune status, somatic mutation, pseudo cell differentiation and drug sensitivity were further explored.Results: A 4-MTRG signature was constructed. Risk score derived from the model was further validated as an independent variable for survival prediction. Two risk groups were classified based on the risk score calculated by the 4-MTRG signature. Additionally, we observed a significant difference in immune cells infiltration, such as subsets of CD4 T cells and macrophages, between the high- and low groups. Moreover, in the pseudo-time analysis, TIMP1 was found to express higher as time goes on. Finally, three small molecule drugs, elesclomol, shikonin and bryostatin-1, exhibited binding potential to TIMP-1.Conclusion: The proposed 4-MTRG signature is a promising biomarker to predict clinical outcomes and therapeutic responses in colon cancer patients.
Perception of risk and compliance with COVID-19 safety guidelines among healthcare workers in Ghana: a cross-sectional study
Authors: Kayi Esinam Afi , Ottie-Boakye Doris , Ansa Gloria Akosua , Appiah-Agyekum Nana Nimo 
Published: June 2022

Abstract
Background: Healthcare workers are the ‘frontline’ in the provision of effective and quality healthcare delivery, yet they are beset with several occupational hazards and risks when providing care especially during a global health crisis. With limited health resources availability during patient care delivery coupled with relatively little evidence on compliance to infection prevention practices, this study investigates healthcare workers’ compliance with COVID-19 safety protocols and identifies factors associated with their perceived risk of COVID-19. Methods: This cross-sectional survey utilized non-probability sampling strategies to obtain primary data from both clinical and non-clinical health workers in various health facilities within four regions of Ghana. Structured questionnaires designed using Google forms and paper-based questionnaires were used. Binary logistic regression analysis was performed to identify the factors associated with the perceived risk of COVID-19.Results: A total of 513 questionnaires were obtained at the end of the study. The results showed that healthcare workers are highly compliant with hand hygiene practices and wearing face masks. Results from the regression analysis showed that the number of working years a healthcare worker had spent, category of health professional, type of health facility, region of work, frequency of COVID-19 test, and compliance with hand hygiene practices were strongly associated with healthcare workers’ perception of risk of COVID-19. Conclusion: Findings suggest that both individual and health system factors are significant in increasing the risk of COVID-19 among healthcare workers. Efforts at enforcing strict compliance with infection prevention should be implemented to protect the health workforce and mitigate against the transmission of the coronavirus.
Work Intensity and Workers' Sleep
Authors: Lyons Sai , Strazdins Lyndall , Doan Tinh 
Published: June 2022

Abstract
Experiencing poor sleep quality affects an individual’s health and wellbeing. Sleep quality may be influenced by work conditions, with working under high intensity a possible contributor. Using data from the Household, Income and Labour Dynamics in Australia (HILDA) survey waves 2013 and 2017, this study investigates whether experiencing poor sleep quality is associated with work intensity. This study uses a nationally representative sample of working Australian individuals aged 25–64 from the HILDA survey and finds a strong positive association between work intensity and sleep quality, with an individual’s sleep quality worsening as their work intensity increased. The association between work intensity and sleep quality is even stronger than that between long work hours and sleep quality.
Admission glucose as a prognostic marker for all-cause mortality and cardiovascular disease
Authors: Djupsjö Catarina , Kuhl Jeanette , Andersson Tomas , Lundbäck Magnus , Holzmann Martin J. , Nyström Thomas 
Published: June 2022

Abstract
Background: Diabetes and prediabetes are known risk factors for cardiovascular disease and associated with increased mortality risk. Whether patients with a random elevated blood glucose level but no history of diabetes are at a higher mortality and cardiovascular risk is not entirely known.Methods: A retrospective cohort study where patients (18-80 years) with no history of diabetes between 2006-2016 attending the emergency department (ED) in Sweden were included. Based on the first (index) blood glucose level patients were categorized into four groups: hypoglycemia (<3.9 mmol/l), normal glucose tolerance (NGT) (3.9-7.8 mmol/l), dysglycemia (7.8-11.1 mmol/l), and hyperglycemia (>11.1 mmol/l). Data was collected from four nationwide registers (National Patient Register, National Cause of Death Register, Prescribed Drug Register and Statistics Sweden). Cox regression was used to calculate adjusted hazard ratios (HR) with 95% confidence intervals (CI) for all-cause mortality and cardiovascular outcomes using NGT as reference.Results: 622018 patients were included during a mean follow-up time of 3.9 years. According to the index blood glucose level: 1877 (0.3%) had hypoglycemia, 527459 (85%) had NGT, 78416 (13%) had dysglycemia, and 14176 (2%) patients had hyperglycemia, respectively. During follow-up 45493 (7.3%) deaths occurred. After multiple adjustments, mortality risk was highest in patients with hypoglycemia HR 2.51 (2.19-2.87) followed by patients with hyperglycemia HR 1.87 (1.80-1.94) and dysglycemia HR 1.23 (1.20-1.26). Risk for cardiovascular events: i.e., myocardial infarction, stroke and heart failure, were highest among patients with hyperglycemia HR 2.26 (2.11-2.41), HR 1.59 (1.48-1.70) and HR 1.62 (1.49-1.77), respectively. Conclusion: Patients with disturbed blood glucose level at ED admission have a higher mortality risk than patients with NGT. Patients with hyperglycemia have almost a two folded increased long-term mortality risk and more than a doubled risk for cardiovascular events compared to patients with NGT.
Nephropathic cystinosis in a Chilean cohort: Clinical and genetic characterization
Authors: Krall Paola , Henríquez Jean Antoine Grandy , Bolte Lillian , Salgado Jennie , Cavagnaro Felipe , Gonzalez Claudia , Guerrero Jose Luis 
Published: June 2022

Abstract
Cystinosis is a rare disease caused by CTNS gene defects. The main clinical presentations are nephropathic infantile cystinosis (NIC) and nephropathic juvenile cystinosis (NJC); both develop chronic kidney disease (CKD) and extrarenal complications. Opportune diagnosis and access to therapy are challenging in developing countries. Objectives: To describe the clinical and genetic profile of all cystinosis patients diagnosed in Chile. Methods: We performed a retrospective review of the clinical records of cystinosis patients diagnosed from 1994 to 2022. Age at diagnosis, glomerular filtration rate, metabolic variables, anthropometric values, treatment, genetics analysis and outcomes were recorded. Results: Nine patients (8NIC/1NJC) were diagnosed. Patients with NIC had a median age of 16.5 (IQR 13-23) months at diagnosis, and two patients died during follow-up. Most patients started cysteamine therapy within 5 months after diagnosis and reached CKD stages 3-4 within four years. Laboratory and clinical evaluations improved after CKD management and cysteamine therapy. During the follow-ups, all but one of the NIC patients showed height for age Z-score values between -1.5 and -4.0. Two patients received kidney transplantation, and one of them still remains functional after 15 years. The single NJC was a 21-year-old female patient who received insufficient cysteamine and rapidly reached CKD stage 4. Genetic testing was positive in 7/7 cases, with del57kb being the predominant variant (10/14 alleles). Conclusion: Developing countries still face many challenges in providing adequate healthcare to cystinosis patients. Opportune genetic testing may facilitate early diagnosis, resulting in better prognoses.