bluuebunny/crossref_metadata_2025_split huggingface dataset:
The exploration of miRNAs and mRNAs profiles revealed the molecular mechanisms of cattle-yak male infertility
Authors: Zhao Shaokang , Sun Wenqiang , Chen Shiyi , Li Yuchao , Wang Jie , Lai Songjia , Jia Xianbo 
Published: May 2022

Abstract
BackgroundCattle-yak, the first-generation offspring of cattle and yak, inherited many excellent characteristics from their parents. However, F1 male hybrid infertility restricts the utilization of heterosis greatly. The coding genes can transcript into such mRNAs and further translate into proteins to maintain the activities of organisms. MiRNAs are endogenous small noncoding RNA that silenced their target mRNA expression by high complementary binding sites. In recent years, mRNA and miRNA profiles produced by high-throughput sequencer had interpreted the molecular mechanisms of numerous biological phenomena. However, based on the cattle-yak male sterility, the comprehensive comparison of testicular tissue RNA profiles of cattle, yak and cattle-yak have not been conducted. ResultsTesticular histology indicated that the seminiferous tubules became vacuolated and there are few active germs cells can be seen. With the RNA-seq analysis we finally identified 47 up-regulated miRNAs and 34 down-regulated miRNAs in cattle, 16 up-regulated miRNAs and 21 down-regulated miRNAs in cattle and yaks compared with cattle-yak, respectively. Furthermore, we found that the known miRNA bta-miR-7 was commonly down-regulated in cattle-yak group, revealed the vital role of miR-7 in the regulation of reproductive processes. With the target prediction, the coding genes MYRFL, FANCA, INSL3, USP9X, SHF became the regulated candidates. The network analysis of GO to GO and pathway to pathway annotated such hub terms and genes related to many regulatory functions. We also performed GSE GO and GSE KEGG to display the function of DE gene sets authenticated in our previous study and found that the gene sets most enriched in ATP binding, DNA binding, reproduction process and MAPK signaling pathway, uncovered the relevance between the DE genes and cattle-yak male infertility. ConclusionsWe first obtained the compared miRNAs profiles of testicular tissue samples among cattle, yak and cattle-yak and analyzed the fundamental functions of their target genes. As a complement, GSEA of mRNA profiles identified in our previous study was also be conducted. This study preliminarily revealed some miRNAs related to reproductive processes such as bta-miR-7 and such critical regulatory pathways. Our research is of great significance to explore the molecular mechanism of cattle-yak infertility and look for strategies to resolve this difficult problem.
Tibial nerve dynamics during ankle dorsiflexion: Using ultrasonographic stiffness to measure excursion
Authors: Anegawa Keisuke , Nakamura Misaki , Izumi Meika , Kawanishi Kengo , Tsutsumi Masahiro , Kudo Shintarou 
Published: May 2022

Abstract
Decreased nerve mobility and increased nerve stiffness have been proposed as causes of tarsal tunnel syndrome and diabetic neuropathy. The purpose of this study was to measure the stiffness of the tibial nerve (TN) at the ankle joint at positions of plantarflexion (PF) and dorsiflexion (DF) and the mobility (excursion) of the TN during dorsiflexion using shear-wave elastography ultrasonography. Twenty-one healthy adults participated in constant-velocity movements of the ankle joint from the maximum dorsiflexion angle to -20°, and the TN was imaged using an ultrasound imaging system. The maximum flow velocity value and the TN excursion distance per dorsiflexion were then calculated as indexes of excursion using the application software Flow PIV. The shear-wave velocities of the TN at PF and DF were also measured. We observed a relationship between the TN excursion and stiffness that was stronger at 25% dorsiflexion than at 75% dorsiflexion. In addition, the ultrasonographic shear-wave velocity could predict the excursion of the TN if measured under mild plantar flexion of the ankle joint (i.e., 25% dorsiflexion).
Cystic angiomatosis in child: clinical experience and review of literatures
Authors: Liu Li , Li Wen Chao , Wang Zhen Dong , Chen Hui , Liu Guang , Feng Zhi Chun 
Published: May 2022

Abstract
Background: Cystic angiomatosis is a rare disease characterized by the proliferation of vascular and lymphatic channels lined by a single layer of endothelial cells. Here, we reported a child of cystic angiomatosis with multifocal lesion in bone, who was diagnosed by biopsy surgery and evaluated by whole exome sequencing. Case Description: In this case presentation, we report a case of 11-year-old boy who had the pain in chest. Computed tomography revealed the multiple lytic of bone in ribs, clavicle, vertebra thoracalis, skull, mandibula, shoulder blade and so on. Blood test showed that ALP was 393U/L with the normal of 20–110 U/L and VEGF was 287.26 pg/ml with the normal of 0-142 pg/ml. The patient was performed by the biopsy surgery in ribs and diagnosed of cystic angiomatosis by pathological examination. The whole exome sequencing showed the single nucleotide substitutions in the coding region including BRIP1, CHEK2, GRM4, and MUC16. Besides, the up-regulated genes were involved of CASC15, CENPF, ABCA13, ALK, BLM, FGFR3.Conclusions: In this article, we report a rare case of cystic angiomatosis in a child with multiple lesions of bone and abnormal VEGF and ALP in peripheral blood examination. Then, the whole exome sequencing could be performed in order to explore the potential molecular mechanism in the development of cystic angiomatosis.
Metastatic colon cancer cured by Traditional Chinese Medicine combined with Chemotherapy: A case report
Authors: Deng Chengeng , Tang Mengyuan , Liu Zhaoheng 
Published: May 2022

Abstract
Introduction: Colon cancer is a kind of disease very common worldwide and one of the leading causes of cancer morbidity and mortality. Traditional Chinese medicine is widely used in varieties of Chronic diseases treatment. Colon cancer results in a high rate of morbidity and mortality and easily metastasizes. Patient concerns: We report a 71-year-old male patient with a more than 12-year history of old myocardial infarction (OMI) and a more than a 7-year history of diabetes mellitus type 2. Diagnoses: The patient was diagnosed with colon cancer and was asked to resect part of right-sided colon cancer one year ago, and biopsy specimens from the tumor suggested a moderately poorly differentiated adenocarcinoma. Anastomosis recurrence and pelvic metastasis occurred in this patient was discovered 2 months later. Meanwhile, the liver damage caused by chemotherapy also slowly developed.Interventions: The patient received adjuvant chemotherapy with XELOX (oxaliplatin and Capecitabine) for 8 months after the Laparoscopic radical resection of the right colon cancer. Then, we advised the patient to take Chinese medicine for his condition to remove the hidden danger of metastatic colon cancer in pelvic.Outcomes: The patient was finally cured of metastatic colon cancer after receiving TCM therapy based on long-term chemotherapy. Conclusion: Traditional Chinese medicine can provide an effective way in the process of metastatic colon cancer.
Bargaining with patriarchy through the life course: obstacles faced (and overcome) by women leaders in Kerala’s health sector
Authors: Nambiar Devaki , Benny Gloria , Sankar Hari 
Published: May 2022

Abstract
Background:The COVID-19 pandemic has helped shine the spotlight on the role of women’s leadership in tackling the world’s health and health system challenges. Women are less often than men occupy positions of leadership in the health sector, even as they may constitute a vast majority of the work force. The South Indian state of Kerala is an exception to this trend, a phenomenon that we sought to understand and contextualise. We undertook a study to understand the personal and professional journeys of some women leaders in the Kerala health sector to determine the antecedents of their leadership positions, the challenges came their way of leadership and strategies adopted to overcome these challenges. We also investigated into how these experiences shaped their styles and approaches to leadership. Methods:We employed a qualitative field research methodology like in- depth interviews among women leaders. Sixteen women leaders were identified from public records and through peer nomination and interviewed in their language of preference following written informed consent procedures. Interviews focused on participants’ professional and personal trajectories, work-life balance, style of leadership, challenges, enablers, lessons learned in their path, and their vision for the health system. Transliterated English transcripts were analysed using a thematic analysis approach and Atlas.Ti8 software by three researchers.Results: Our study participants were aged 40 to around 80 years, from 8 out of 14 districts of the state. Women leaders in Kerala’s health sector faced challenges through the life-course: during their early school education, in professional service as well as in their roles as leaders. There were myriad experiences – including gender stereotyping and discrimination at the intersection of gender and other social identities. Women developed manifold ways of overcoming them and evolve unique – and again myriad - leadership styles. Conclusions: Women leaders in Kerala have faced shared challenges through their life-course to climb up the ranks of leadership and have adopted manifold ways of overcoming them with myriad leadership styles. This reflects various patterns of bargaining with patriarchy, which in turn suggests a feminist consciousness on the part of Kerala women leaders as well as the society in which they are seeking to lead.
Effects of environmentally relevant concentrations of sertraline on the antipredator behavior of Scinax fuscovarius tadpoles
Authors: Barsoumian Hagop Armenio , Martins Aline Franco , Costa José Luiz , Schiesari Luis 
Published: May 2022

Abstract
Sertraline is one of the most commonly detected antidepressants in surface waters, raising concerns on potential, non-intended effects on the behavior of non-target organisms. In this study we experimentally evaluate whether sertraline, at environmentally relevant concentrations, influence the behavior of prey in response to their predators. Our model system comprised tadpoles of the treefrog Scinax fuscovarius as consumers, and larvae of the dragonfly Erythrodiplax sp as predators. This prey-predator system was exposed to sertraline concentrations of 0, 0.1 and 1.0 ug/L. We quantified tadpole activity rates, food intake rates, spatial avoidance, and hiding behavior, all of which are known to regulate predation risk. No main effects of sertraline were observed for any response variable; a main effect of predator presence was observed for spatial avoidance only. Most importantly, a significant sertraline-by-predator presence interaction term suggests that activity rates face to predator presence is influenced by sertraline. We in addition quantified response variables related to swimming kinematics, which are also known to regulate predation risk. Tadpoles exposed to sertraline spent less time swimming, swam for a shorter distance, and demanded more stimuli to initiate swimming. Overall, our results suggest that environmental contamination by sertraline may influence larval amphibian behavior in ways that could influence their fitness.
Acquired peripheral nerve injuries associated with severe COVID-19
Authors: Franz Colin K. , Murthy Nikhil K. , Malik George R. , Kwak Jean W. , D’Andrea Dom , Wolfe Alexis R. , Farr Ellen , Stearns Melanie A. , Deshmukh Swati , Tavee Jinny O. , Sun Fang , Swong Kevin N. , Rydberg Leslie , Cotton R. James , Wolfe Lisa F. , Walter James M. , Coleman John M. , Rogers John A. 
Published: May 2022

Abstract
We diagnosed 66 peripheral nerve injuries in 34 patients who survived severe coronavirus disease 2019 (COVID-19). We combine our latest data with published case series re-analyzed here (117 nerve injuries; 58 patients) to provide a comprehensive accounting of lesion sites. The most common are ulnar (25.1%), common fibular (15.8%), sciatic (13.1%), median (9.8%), brachial plexus (8.7%) and radial (8.2%) nerves at sites known to be vulnerable to mechanical loading. Protection of peripheral nerves should be prioritized in the care of COVID-19 patients. To this end, we report proof of concept data of a wearable, wireless pressure sensor to provide real time monitoring in the intensive care unit setting.
An estriol-eluting pessary to treat pelvic organ prolapse
Authors: Long Jingjunjiao , Zidan Ghada , Seyfoddin Ali , Tong Stephen , Brownfoot Fiona C. , Chowdary Prathima 
Published: May 2022

Abstract
Pelvic organ prolapse affects up to 50% of parous women. Commonly used treatment options have unwelcome attributes; pessaries can cause erosion and estrogen creams need to be applied frequently, which is inconvenient and difficult to administer. This study involved the development of an estriol-releasing pessary utilising 3D printing molds. We incorporated varying amounts of estriol (1%, 10% and 15%) into the silicone pessary. We optimised the mechanical aspects of the pessary so it had a similar strength to commercially available pessaries. We investigated estriol release from the pessary over 3 months. We explored possible interactions between the drug and polymers via FTIR. The MED-4870 silicone ring with similar mechanical strength to pessaries currently used to treat pelvic organ prolapse. The medical pessaries present a sustained release in simulated vaginal fluid over 3 months. The pessary with 10% estriol delivered the optimal dose at 0.8mg each week. Mechanical strength of this pessary showed no difference after emersion in simulated vaginal fluid for 3-month, supporting the long-term application. An estriol-loaded pessary was successfully developed to treat pelvic organ prolapse with sustained release of estriol over 3 months. This pessary provides promising potential to treat pelvic organ prolapse and vaginal atrophy.
Association between surgical tracheostomy and chronic tracheal stenosis: a retrospective, single-center study
Authors: Kuwabara Yuki , Yamakawa Kentaro , Okui Seiko , Miyazaki Erica , Uezono Shoichi 
Published: May 2022

Abstract
Background: Tracheal stenosis is a major complication of tracheostomy. Hence, a smaller endotracheal tube (ETT) tends to be selected for patients with a prior tracheostomy history. However, it likely comes from our trial and error, not scientific evidence, because tracheal stenosis after conventional surgical tracheostomy remains relatively uncharacterized. In this study, we retrospectively examined the association between conventional surgical tracheostomy and tracheal shape as assessed by transverse computed tomography (CT).Methods: Patients who underwent surgery for head and neck cancer during the period January 2010 to December 2013, with a temporary tracheostomy closed within 3 months, were included. Exclusion criteria were tracheostoma before surgery, permanent tracheostomy, or insufficient CT follow-up. Transverse CT slices were measured 2 cm above and below the tracheostomy site (0.5 cm/slice for a total of 9 slices). The minimum cross-sectional tracheal area and horizontal and vertical diameters in transverse CT slices were compared before (baseline [BL]) and 6 months (6M) and 12 months (12M) after tracheostomy. Tracheal stenosis was defined as a decrease in the minimum cross-sectional tracheal area compared to BL.Results: Of 112 patients, 77 were included. The minimum tracheal area was significantly decreased at 6M and 12M compared to BL (BL: mean 285 [SD 68] mm2, 6M: 267 [70] mm2, P <0.01 vs. BL, 12M: 269 [68] mm2, P <0.01 vs. BL), and the localization was predominantly at or above the tracheostomy site at 6M and 12M. Tracheal stenosis was identified in 55 patients at 6M and in 49 patients at 12M without any respiratory symptoms. With regard to horizontal and vertical diameter, only horizontal diameter was significantly decreased at 6M and 12M compared to BL (BL: 16.8 [2.4] mm, 6M: 15.4 [2.7] mm, P <0.01 vs. BL, 12M: 15.6 [2.8] mm, P <0.01 vs. BL).Conclusions: Conventional surgical tracheostomy was associated with decreased horizontal diameter of the trachea (triangulation) and which resulted in decreased cross-sectional tracheal area in more than one-half of the patients without any respiratory symptoms. In patients with prior tracheostomy, regardless of the presence or absence of respiratory symptoms, use of a smaller ETT should be considered.
Immune gene expression analysis indicates the potential of a self-amplifying Covid-19 mRNA vaccine
Authors: Ooi Eng Eong , Ong Eugenia , Yee Jia Xin , Ooi Justin , Syenina Ayesa , de Alwis Ruklanthi , Chen Shiwei , Sim Jean , Kalimuddin Shirin , Leong Yan Shan , Chan Yvonne , Sekulovich Rose , Sullivan Brian , Lindert Kelly , Sullivan Sean , Chivukula Pad , Hughes Steve , Low Jenny , Chan Kuan Rong 
Published: May 2022

Abstract
Remarkable potency has been demonstrated for mRNA vaccines in reducing the global burden of the ongoing COVID-19 pandemic. This vaccine platform has been extended to encode an alphavirus replicase that self-amplifies the full length mRNA and SARS-CoV-2 spike (S) transgene (self-amplifying mRNA or sa mRNA). However, early phase clinical trials of sa-mRNA COVID-19 vaccine candidates have questioned the potential of this platform to develop potent vaccines. We examined the immune gene response to a candidate sa-mRNA vaccine against COVID-19, and compared our findings to the host response to other forms of vaccines. In blood samples from healthy volunteers that participated in a phase I/II clinical trial, greater induction of transcripts involved in Toll-like receptor (TLR) signalling, antigen presentation and complement activation at 1 day post-vaccination was associated with higher anti-S antibody titers. Conversely, transcripts involved in T-cell maturation at day 7 post-vaccination informed the magnitude of eventual anti-S T-cell responses. The transcriptomic signature for ARCT-021 vaccination strongly correlated with live viral vector vaccines, adjuvanted vaccines and BNT162b2. Correlation with live attenuated vaccines was weaker. Our findings suggest the potential for sa-mRNA to be further developed to be among the most potent forms of vaccines in our arsenal to prevent infectious diseases.
Workplace violence against nursing interns and patient safety: The multiple mediation effect of professional identity and professional burnout
Authors: Yang Qianqian , Yang Linlin , Yang Chunling , Wu Xia , Chen Yue , Yao Pingping 
Published: May 2022

Abstract
Background: Most nursing interns indicated they had experienced some form of workplace violence on clinical placement, which could be linked to higher adverse events. Prior researches have examined the effects of workplace violence on patient safety, but there have been very few explorations of whether workplace violence affects patient safety through professional identity and burnout among nursing interns. The purpose of this study is to test whether professional identity and professional burnout play mediating roles in the relationship of workplace violence and patient safety among nursing interns. Methods: Our study used a cross-sectional design. The study consisted of 466 nursing interns from three tertiary grade A hospitals in Shandong Province, China. The Chinese version of the Workplace Violence Scale, the Professional Identity Scale, the Chinese version of the Maslach Burnout Inventory-General Survey, the Patient Safety Behavior Scale were used to gather data. Descriptive analysis, t tests, analysis of variance, Pearson correlation, the serial multiple mediation analysis were used to analyze data. Results: Workplace violence, professional identity, professional burnout and patient safety were significantly correlated (p < 0.01). Workplace violence can not only have a direct positive impact on patient safety of the nursing interns (effect=-0.3280;SE=0.1492;95%CI: LL=-0.6212, UL=-0.0347), but also have an indirect impact on the patient safety through three paths: the independent mediating role of professional identity (effect=-0.3070;SE=0.1282;95%CI: LL=-0.6495, UL=-0.1399), the independent mediating role of professional burnout(effect=-0.1151;SE=0.0432;95%CI: LL=-0.2246, UL=-0.0456), and the chain mediating role of professional identity and professional burnout (effect=-0.1432;SE=0.0762;95%CI: LL=-0.3338, UL=-0.0331). Conclusion: Workplace violence first had a negative correlation with professional identity and then increased professional burnout, which was in turn related to reduce nursing interns-reported patient safety.
Inflammation and immune activation are associated with risk of Mycobacterium tuberculosis infection in BCG-vaccinated infants
Authors: Satti Iman , Wittenberg Rachel , Li Shuailin , Harris Stephanie , Tanner Rachel , Cizmeci Deniz , Jacobs Ashley , Williams Nicola , Mulenga Humphrey , Fletcher Helen , Scriba Thomas , Tameris Michele , Hatherill Mark , McShane Helen 
Published: May 2022

Abstract
Tuberculosis vaccine development is hindered by the lack of validated immune correlates of protection. Exploring immune correlates of risk of disease and/or infection in prospective samples can inform this field. We investigated whether previously identified immune correlates of risk of TB disease also associate with increased risk of M.tb infection in BCG-vaccinated South African infants, who became infected with M.tb during 2–3 years of follow-up. M.tb infection was defined by conversion to positive reactivity in the QuantiFERON test. We demonstrate that inflammation and immune activation are associated with risk of M.tb infection. Ag85A-specific IgG was elevated in infants that were subsequently infected with M.tb, and this was coupled with upregulated gene expression of immunoglobulin-associated genes and type-I interferon. Plasma levels of IFN-\(\alpha\)2, TNF-\(\alpha\), CXCL10 (IP-10) and complement C2 were also higher in infants that were subsequently infected with M.tb.