bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of subsequent malignancies after total body irradiation-based allogeneic HSCT in children with ALL – long-term follow-up from the prospective ALL-SCT 2003 trial
Authors: Eichinger Anna , Poetschger Ulrike , Glogova Evgenia , Bader Peter , Basu Oliver , Beier Rita , Burkhardt Birgit , Classen Carl-Friedrich , Claviez Alexander , Corbacioglu Selim , Deubzer Hedwig E. , Greil Johann , Gruhn Bernd , Güngör Tayfun , Kafa Kinan , Kühl Jörn-Sven , Lang Peter , Lange Bjoern Soenke , Meisel Roland , Müller Ingo , Sauer Martin G. , Schlegel Paul-Gerhardt , Schulz Ansgar , Stachel Daniel , Strahm Brigitte , Wawer Angela , Peters Christina , Albert Michael H. 
Published: September 2022

AbstractTotal body irradiation (TBI)-based conditioning is associated with superior leukemia-free survival in children with ALL undergoing HSCT. However, the risk for subsequent malignant neoplasms (SMN) remains a significant concern. We analyzed 705 pediatric patients enrolled in the prospective ALL-SCT-BFM-2003 trial and its subsequent registry. Patients >2 years received conditioning with TBI 12 Gy/etoposide (n = 558) and children ≤2 years of age or with contraindications for TBI received busulfan/cyclophosphamide/etoposide (n = 110). The 5- and 10-year cumulative incidence of SMN was 0.02 ± 0.01 and 0.13 ± 0.03, respectively. In total, 39 SMN (34 solid tumors, 5 MDS/AML) were diagnosed in 33 patients at a median of 5.8 years (1.7–13.4), exclusively in the TBI group. Of 33 affected patients, 21 (64%) are alive at a median follow-up of 5.1 years (0–9.9) after diagnosis of their first SMN. In univariate analysis, neither age at HSCT, donor type, acute GVHD, chronic GVHD, nor CMV constituted a significant risk factor for SMN. The only significant risk factor was TBI versus non-TBI based conditioning. This analysis confirms and quantifies the increased risk of SMN in children with ALL after conditioning with TBI. Future strategies to avoid TBI will need careful tailoring within prospective, controlled studies to prevent unfavorable outcomes.
Polyvinyl chloride degradation by a bacterium isolated from the gut of insect larvae
Authors: Zhang Zhe , Peng Haoran , Yang Dongchen , Zhang Guoqing , Zhang Jinlin , Ju Feng 
Published: September 2022

AbstractEvidence for microbial degradation of polyvinyl chloride (PVC) has previously been reported, but little is known about the degrading strains and enzymes. Here, we isolate a PVC-degrading bacterium from the gut of insect larvae and shed light on the PVC degradation pathway using a multi-omic approach. We show that the larvae of an insect pest, Spodoptera frugiperda, can survive by feeding on PVC film, and this is associated with enrichment of Enterococcus, Klebsiella and other bacteria in the larva’s gut microbiota. A bacterial strain isolated from the larval intestine (Klebsiella sp. EMBL-1) is able to depolymerize and utilize PVC as sole energy source. We use genomic, transcriptomic, proteomic, and metabolomic analyses to identify genes and proteins potentially involved in PVC degradation (e.g., catalase-peroxidase, dehalogenases, enolase, aldehyde dehydrogenase and oxygenase), and propose a PVC biodegradation pathway. Furthermore, enzymatic assays using the purified catalase-peroxidase support a role in PVC depolymerization.
A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging
Authors: Wang Liang , Wu Chunling , Peng Wanling , Zhou Ziliang , Zeng Jianzhi , Li Xuelin , Yang Yini , Yu Shuguang , Zou Ye , Huang Mian , Liu Chang , Chen Yefei , Li Yi , Ti Panpan , Liu Wenfeng , Gao Yufeng , Zheng Wei , Zhong Haining , Gao Shangbang , Lu Zhonghua , Ren Pei-Gen , Ng Ho Leung , He Jie , Chen Shoudeng , Xu Min , Li Yulong , Chu Jun 
Published: September 2022

AbstractcAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current genetically encoded fluorescent cAMP indicators. Here, we report the development of the new circularly permuted GFP (cpGFP)-based cAMP indicator G-Flamp1, which exhibits a large fluorescence increase (a maximum ΔF/F0 of 1100% in HEK293T cells), decent brightness, appropriate affinity (a Kd of 2.17 μM) and fast response kinetics (an association and dissociation half-time of 0.20 and 0.087 s, respectively). Furthermore, the crystal structure of the cAMP-bound G-Flamp1 reveals one linker connecting the cAMP-binding domain to cpGFP adopts a distorted β-strand conformation that may serve as a fluorescence modulation switch. We demonstrate that G-Flamp1 enables sensitive monitoring of endogenous cAMP signals in brain regions that are implicated in learning and motor control in living organisms such as fruit flies and mice.
Localized interlayer excitons in MoSe2–WSe2 heterostructures without a moiré potential
Authors: Mahdikhanysarvejahany Fateme , Shanks Daniel N. , Klein Matthew , Wang Qian , Koehler Michael R. , Mandrus David G. , Taniguchi Takashi , Watanabe Kenji , Monti Oliver L. A. , LeRoy Brian J. , Schaibley John R. 
Published: September 2022

AbstractInterlayer excitons (IXs) in MoSe2–WSe2 heterobilayers have generated interest as highly tunable light emitters in transition metal dichalcogenide (TMD) heterostructures. Previous reports of spectrally narrow (<1 meV) photoluminescence (PL) emission lines at low temperature have been attributed to IXs localized by the moiré potential between the TMD layers. We show that spectrally narrow IX PL lines are present even when the moiré potential is suppressed by inserting a bilayer hexagonal boron nitride (hBN) spacer between the TMD layers. We compare the doping, electric field, magnetic field, and temperature dependence of IXs in a directly contacted MoSe2–WSe2 region to those in a region separated by bilayer hBN. The doping, electric field, and temperature dependence of the narrow IX lines are similar for both regions, but their excitonic g-factors have opposite signs, indicating that the origin of narrow IX PL is not the moiré potential.
An RNA aptamer that shifts the reduction potential of metabolic cofactors
Authors: Samuelian John S. , Gremminger Thomas J. , Song Zhenwei , Poudyal Raghav R. , Li Jun , Zhou Yuanzhe , Staller Seth A. , Carballo Johan A. , Roychowdhury-Saha Manami , Chen Shi-Jie , Burke Donald H. , Heng Xiao , Baum Dana A. 
Published: September 2022

AbstractThe discovery of ribozymes has inspired exploration of RNA’s potential to serve as primordial catalysts in a hypothesized RNA world. Modern oxidoreductase enzymes employ differential binding between reduced and oxidized forms of redox cofactors to alter cofactor reduction potential and enhance the enzyme’s catalytic capabilities. The utility of differential affinity has been underexplored as a chemical strategy for RNA. Here we show an RNA aptamer that preferentially binds oxidized forms of flavin over reduced forms and markedly shifts flavin reduction potential by −40 mV, similar to shifts for oxidoreductases. Nuclear magnetic resonance structural analysis revealed π–π and donor atom–π interactions between the aptamer and flavin that cause unfavorable contacts with the electron-rich reduced form, suggesting a mechanism by which the local environment of the RNA-binding pocket drives the observed shift in cofactor reduction potential. It seems likely that primordial RNAs could have used similar strategies in RNA world metabolisms.
Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer
Authors: Sugimoto Yoichiro , Ratcliffe Peter J. 
Published: September 2022

AbstractHypoxia inducible factor (HIF) and mammalian target of rapamycin (mTOR) pathways orchestrate responses to oxygen and nutrient availability. These pathways are frequently dysregulated in cancer, but their interplay is poorly understood, in part because of difficulties in simultaneous measurement of global and mRNA-specific translation. Here, we describe a workflow for measurement of ribosome load of mRNAs resolved by their transcription start sites (TSSs). Its application to kidney cancer cells reveals extensive translational reprogramming by mTOR, strongly affecting many metabolic enzymes and pathways. By contrast, global effects of HIF on translation are limited, and we do not observe reported translational activation by HIF2A. In contrast, HIF-dependent alterations in TSS usage are associated with robust changes in translational efficiency in a subset of genes. Analyses of the interplay of HIF and mTOR reveal that specific classes of HIF1A and HIF2A transcriptional target gene manifest different sensitivity to mTOR, in a manner that supports combined use of HIF2A and mTOR inhibitors in treatment of kidney cancer.
Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1
Authors: Prattes Michael , Grishkovskaya Irina , Hodirnau Victor-Valentin , Hetzmannseder Christina , Zisser Gertrude , Sailer Carolin , Kargas Vasileios , Loibl Mathias , Gerhalter Magdalena , Kofler Lisa , Warren Alan J. , Stengel Florian , Haselbach David , Bergler Helmut 
Published: September 2022

AbstractThe AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The molecular mechanism of release remained elusive. Here, we report a series of cryo-EM structures that captured the extraction of Rlp24 from pre-60S particles by Saccharomyces cerevisiae Drg1. These structures reveal that Arx1 and the eukaryote-specific rRNA expansion segment ES27 form a joint docking platform that positions Drg1 for efficient extraction of Rlp24 from the pre-ribosome. The tips of the Drg1 N domains thereby guide the Rlp24 C terminus into the central pore of the Drg1 hexamer, enabling extraction by a hand-over-hand translocation mechanism. Our results uncover substrate recognition and processing by Drg1 step by step and provide a comprehensive mechanistic picture of the conserved modus operandi of AAA-ATPases.
Trizma as an eco-friendly efficient inhibitor for the acidic corrosion of steel: experimental and computational studies
Authors: Abd-El-Nabey B. A. , El-Housseiny S. , Abd-El-Fatah M. A. 
Published: September 2022

AbstractThe inhibition characteristics of Trizma for corrosion of steel in 1 M HCl was investigated using the weight loss, potentiodynamic polarization, electrochemical impedance spectroscopy techniques and the surface techniques XRD,SEM and EDX. The potentiodynamic results indicated that Trizma act as a mixed type inhibitor for steel in 1 M HCl giving efficiently 93.7% percent inhibition for 1 × 10–2 mol/L. The electrochemical impedance spectroscopy results showed an increase inRctvalues and decrease in the value ofCdlwith increasing the concentration of Trizma indicating that the presence of Trizma in the solution retards the steel corrosion due to the adsorption of its molecules at the steel/solution interface. The XRD and SEM results indicated that the surface of the steel contains Trizma molecules. The DFT method was investigated to correlate the molecular properties of the studied Trizma with the experimental inhibition efficiency. Langmuir, Flory–Huggins isotherm, and the Kinetic–thermodynamic model were used to fit the corrosion inhibition data of Trizma. The results indicated that the Langmuir isotherm does not fit with the experimental results due mainly to the non-ideal adsorption of its molecules at the steel/solution interface. However, Flory–Huggins isotherms, and the Kinetic–thermodynamic model are applicable and showed that the adsorption process of Trizma on the steel surface is cooperative (Chemical–Physical).
Diffusion of punishment in collective norm violations
Authors: Keshmirian Anita , Hemmatian Babak , Bahrami Bahador , Deroy Ophelia , Cushman Fiery 
Published: September 2022

AbstractPeople assign less punishment to individuals who inflict harm collectively, compared to those who do so alone. We show that this arises from judgments of diminished individual causal responsibility in the collective cases. In Experiment 1, participants (N = 1002) assigned less punishment to individuals involved in collective actions leading to intentional and accidental deaths, but not failed attempts, emphasizing that harmful outcomes, but not malicious intentions, were necessary and sufficient for the diffusion of punishment. Experiments 2.a compared the diffusion of punishment for harmful actions with ‘victimless’ purity violations (e.g., eating a dead human’s flesh as a group; N = 752). In victimless cases, where the question of causal responsibility for harm does not arise, diffusion of collective responsibility was greatly reduced—an outcome replicated in Experiment 2.b (N = 479). Together, the results are consistent with discounting in causal attribution as the underlying mechanism of reduction in proposed punishment for collective harmful actions.
Molecular composition of soil organic matter (SOM) regulate qualities of tobacco leaves
Authors: Zhai Xu , Zhang Long , Wu Ruofan , Wang Mei , Liu Yanxiang , Lian Jiapan , Munir Mehr Ahmed Mujtaba , Chen Dan , Liu Lei , Yang Xiaoe 
Published: September 2022

AbstractSoil organic matter (SOM) is of vital importance to soil health, and also plays a crucial role in the quality of the crops such as tobacco. However, the link between tobacco quality and SOM chemical compositions is still not well understood. To fill the information gap, we analyzed the quality of tobacco leaves and the corresponding SOM molecular compositions by electrospray ionization (ESI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS), that were collected from six different sites in Bijie, Guizhou Province, China. The tobacco quality variedin six sites based on their chemical compositions. SOM compounds had a remarked impact on the quality of tobacco leaves and a distinct difference in SOM composition between low-quality and high-quality tobacco leaves was observed as well. Specifically, 105 common molecular formulas were detected in three SOM compounds of high-quality tobacco, which were more than those in low-quality samples. Although amino sugar, proteins, lipids, tannins, and carbohydrates had a collective influence on the chemical composition of tobacco leaves, the effect contributed by amino sugar and tannins was more prominent. In summary, fully understanding the association between tobacco chemical composition and SOM compounds can provide new insight into the regulation of tobacco quality and the sustainable development of agriculture.
DACBT: deep learning approach for classification of brain tumors using MRI data in IoT healthcare environment
Authors: Haq Amin ul , Li Jian Ping , Khan Shakir , Alshara Mohammed Ali , Alotaibi Reemiah Muneer , Mawuli CobbinahBernard 
Published: September 2022

AbstractThe classification of brain tumors (BT) is significantly essential for the diagnosis of Brian cancer (BC) in IoT-healthcare systems. Artificial intelligence (AI) techniques based on Computer aided diagnostic systems (CADS) are mostly used for the accurate detection of brain cancer. However, due to the inaccuracy of artificial diagnostic systems, medical professionals are not effectively incorporating them into the diagnosis process of Brain Cancer. In this research study, we proposed a robust brain tumor classification method using Deep Learning (DL) techniques to address the lack of accuracy issue in existing artificial diagnosis systems. In the design of the proposed approach, an improved convolution neural network (CNN) is used to classify brain tumors employing brain magnetic resonance (MR) image data. The model classification performance has improved by incorporating data augmentation and transfer learning methods. The results confirmed that the model obtained high accuracy compared to the baseline models. Based on high predictive results we suggest the proposed model for brain cancer diagnosis in IoT-healthcare systems.
Attenuation of allergenicity of roasted cod with Allium spp.: characterization of principal anti-allergenic constituent and action mechanism
Authors: Wang Qi , Liu Xiaobing , Cheng Weiwei , Wang Xiaowen , Chen Feng , Cheng Ka-Wing 
Published: September 2022

Pretreatment with Allium powders could attenuate allergenicity of roasted cod. Among five Allium spp. evaluated, Chinese chive is the most promising, and mangiferin is identified to be one of its major antiallergenic constituents.
Stability of Vestibular Testing in Children With Hearing Loss
Authors: Patterson Jessie N. , Chen Su , Janky Kristen L. 
Published: September 2022

Purpose:
The purpose of this study was to evaluate the stability of rotary chair, video head impulse test (vHIT), and vestibular evoked myogenic potential (VEMP) responses in children with normal hearing (NH) and children with cochlear implants (CIs).

Method:

Retrospective analysis of 66 children (33 males,
M
age
= 11.4 years, range: 3–18 years) seen in a tertiary clinic and/or research laboratory who completed rotary chair, VEMP, and vHIT across two test sessions between 2012 and 2019. The stability of these measures was compared between two groups: children with NH (
n
= 35) and children with CI (
n
= 31). For each outcome, the session difference was calculated by subtracting Session 1 from Session 2.

Results:
For rotary chair (gain and phase) and vHIT (gain), linear mixed-effects models revealed that there were no significant interactions or main effects for group (CI vs. NH), time between session, gender, or age on the session difference, suggesting that the outcomes of these measures are stable across sessions. For cervical and ocular VEMP amplitude, there was a significant interaction between group and time between sessions on the session difference. Specifically, children with NH demonstrated larger amplitudes at Session 2, whereas children with CI demonstrated smaller amplitudes at Session 2. Next, test findings were classified as normal, unilaterally abnormal, or bilaterally abnormal for Sessions 1 and 2. Misclassification was defined as a mismatch of classification between sessions. Rotary chair and vHIT had the fewest misclassifications, whereas cervical VEMPs had the most misclassifications in children with CI and ocular VEMPs had the most misclassifications in children with NH. Misclassifications in children with CI were mostly consistent with progressive vestibular loss, whereas misclassifications in children with NH were mostly consistent with improved vestibular function.

Conclusions:
Stability and misclassification rates varied between tests and groups. Overall, rotary chair and vHIT outcomes were stable in both groups; however, VEMPs differentially changed between groups, improving in children with NH and declining in children with CI. Furthermore, despite relative stability, some children with CI evidenced progressive vestibular loss on all measures suggesting that vestibular testing should be completed serially due to the possibility of progression.
Mit intermittierender pneumatischer Kompression Amputationen abwenden – am Beispiel eines Ulcus hypertonicum (Martorell)
Authors: Kröger Lasse , Dräger Sören , Kahle Birgit 
Published: September 2022

ZusammenfassungIn diesem Fall berichten wir von einer Patientin mit Ulcus cruris hypertonicum Martorell,
bei welcher sich in einem mehrjährigen Zeitraum Ulzera sequenziell an beiden Unterschenkeln
entwickelten. Zwei Jahre vor der Vorstellung in unserer Klinik war das initial betroffene Bein
bereits auf Oberschenkelhöhe amputiert worden. Zum Zeitpunkt der Vorstellung zeigte sich am
verbliebenen Bein eine progrediente schmerzhafte Ulzeration. Unter einer, bei der
Arbeitsdiagnose eines Pyoderma gangraenosums, initial begonnenen immunsuppressiven
Dexamethason-Pulstherapie zeigte sich eine deutliche Verschlechterung des Befundes, sodass die
Diagnose revidiert wurde und eine Therapie mit intermittierender pneumatischer Kompression
begonnen wurde. Hierauf zeigte sich eine langsame Abheilung des Ulkus. Der Fall zeigt den
Stellenwert einer adäquaten Behandlung mittels intermittierender pneumatischer Kompression zur
Vermeidung einer Amputation.
Expeditious Synthesis of Fluorescent Bis(phenylfuryl)benzenes
Authors: Koo Sangho , Zhang Miao , Wang Tingshu , Boo Chanyoung 
Published: September 2022

AbstractVersatile fluorescent polyaromatic compounds of alternating benzene and furan rings, para- and meta-bis(5-phenylfur-3-yl)benzenes, were expeditiously synthesized by double Mn(OAc)3-catalyzed oxidative decarbonylation and Paal–Knorr reactions of β-ketoesters, prepared from the conjugate addition of ethyl acetoacetate to para- and meta-bis(chalcones). Stronger fluorescence intensity was observed for meta-bis(phenylfuryl)benzene with ortho-methoxy substitution, whereas longer fluorescence wavelength was observed for para-bis(phenylfuryl)benzene with para-methoxy substitution.
Breast Cancer Subtypes Classification with Hybrid Machine Learning Model
Authors: Sarkar Suvobrata , Mali Kalyani 
Published: September 2022

Abstract
Background Breast cancer is the most prevailing heterogeneous disease among females characterized with distinct molecular subtypes and varied clinicopathological features. With the emergence of various artificial intelligence techniques especially machine learning, the breast cancer research has attained new heights in cancer detection and prognosis.
Objective Recent development in computer driven diagnostic system has enabled the clinicians to improve the accuracy in detecting various types of breast tumors. Our study is to develop a computer driven diagnostic system which will enable the clinicians to improve the accuracy in detecting various types of breast tumors.
Methods In this article, we proposed a breast cancer classification model based on the hybridization of machine learning approaches for classifying triple-negative breast cancer and non-triple negative breast cancer patients with clinicopathological features collected from multiple tertiary care hospitals/centers.
Results The results of genetic algorithm and support vector machine (GA-SVM) hybrid model was compared with classics feature selection SVM hybrid models like support vector machine-recursive feature elimination (SVM-RFE), LASSO-SVM, Grid-SVM, and linear SVM. The classification results obtained from GA-SVM hybrid model outperformed the other compared models when applied on two distinct hospital-based datasets of patients investigated with breast cancer in North West of African subcontinent. To validate the predictive model accuracy, 10-fold cross-validation method was applied on all models with the same multicentered datasets. The model performance was evaluated with well-known metrics like mean squared error, logarithmic loss, F1-score, area under the ROC curve, and the precision–recall curve.
Conclusion The hybrid machine learning model can be employed for breast cancer subtypes classification that could help the medical practitioners in better treatment planning and disease outcome.