bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative intravenous iron in general surgical patients with iron deficiency anaemia: an observational, retrospective study
Authors: Brown Neil , Yan Ruoling , Henderson Jenniffer , Lowe Genevieve 
Published: May 2022

Abstract
Background: Preoperative iron deficiency anaemia (IDA) is common in the surgical population. Our institution has implemented a pathway for the treatment of IDA using intravenous iron. The impact of this pathway on patient outcomes is assessed in this observational, retrospective cohort study. Methods: Data were collected on patients undergoing elective general or colorectal surgery during 2020 at a tertiary referral centre in the west of Scotland, United Kingdom. Analysis was undertaken to assess the change in haemoglobin level in response to iron administration and any associated impact on blood transfusion requirements, hospital length of stay and readmission rates.Results: A total of 178 patients were included in the analysis, 43% of whom were anaemic preoperatively. Intravenous iron was administered to 26 patients, 92% of whom were iron deficient. An average haemoglobin increment of 22 g/l was observed in patients who received intravenous iron by the time they attended for surgery, and those patients had better recovery of their haemoglobin postoperatively. No statistically significant differences were observed in the rates of postoperative blood transfusion, hospital length of stay or readmission to hospital within 30 days.Conclusions: Although administration of intravenous iron preoperatively was associated with an improvement in haemoglobin levels, it did not appear to be associated with improved hospital outcomes. Further evaluation is required to refine our preoperative anaemia pathway.
Serum vitamin D levels and acute kidney injury: A systemic review and meta-analysis
Authors: Zhang Huanran , Jiang Yan , Shi Nan , Lu Yuan Qiang 
Published: May 2022

Abstract
Background: Numerous researches have evaluated the prevalence and clinical outcome of vitamin D deficiency in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). But the quantitative vitamin D status in acute kidney injury (AKI) patients and its relationship with prognosis remains controversial. We conducted this systemic review and meta-analysis to assess the quantitative difference of vitamin D status, including serum 25(OH) D and 1,25(OH)2D levels，between AKI patients and non-AKI controls, and further explore whether vitamin D status can be clearly correlated with the mortality of AKI. Methods: Major databases, including PubMed, Web of Science and EBSCO, were searched until 1st September 2021. All published observational studies related to vitamin D and AKI According to predefined inclusion and exclusion criteria were extracted. Meta-analyses were performed using Review Manager 5.3.5. Results: Four studies including five cohorts were included with a total of 413 patients. The serum 25(OH)D levels showed no statistically significant difference in AKI patients and non-AKI controls. On the other hand, the serum 1,25(OH)2D levels were significant lower in AKI patients than in non-AKI controls (MD=-17.79, 95% CI =-32.73 to -2.85, P = 0.02). As for the relationship between serum vitamin D status and AKI patients’ mortality, we were unable to give a consistent conclusion based on current limited and conflict study results. Conclusion: Our meta-analysis suggested that serum 1,25(OH)2D levels, rather than 25(OH)D, is significantly lower in AKI patients. The relationship between vitamin D status and clinical outcome of AKI remains controversial based on current evidence. Future comprehensive studies are required to confirm these relations and to elucidate potential mechanisms.
KAT7 promotes radioresistance through upregulating PI3K/AKT signaling in breast cancer
Authors: Ma Ya , Chen Xiaohua , Ding Ting , Zhang Hanqun , Zhang Qiuning , Dai Huanyu , Zhang Haibo , Tang Jianming , Wang Xiaohu 
Published: May 2022

Abstract
Chromatin-modifying enzymes are commonly altered in cancers, but the molecular mechanism by which they regulate cancers remains poorly understood. Herein, we demonstrated that KAT7, a lysine acetyltransferase, was upregulated in breast cancer. KAT7 expression negatively correlated with the survival of breast cancer patients, and KAT7 silencing suppressed breast cancer radioresistance in vitro. Mechanistically, KAT7 activated PIK3CA transcription, leading to enhanced PI3K/AKT signaling and radioresistance. Overexpression of AKT or PIK3CA restored radioresistance suppression induced by KAT7 inhibition. Moreover, overexpression of KAT7, but not KAT7 acetyltransferase activity-deficient mutants promoted AKT phosphorylation, PIK3CA expression, and radioresistance suppression due to KAT7 inhibition. In conclusion, KAT7 has huge prospects for clinical application as a new target for predicting radioresistance in breast cancer patients.
Meta-analysis of clinical trials of transcranial magnetic stimulation for chronic pelvic pain syndrome
Authors: Wang Mengyang , Yang Chunhua , Xia Rui , Xu Zhengxian , Liu Jun , Dai Yalan , Kuang Yuxing , Li Xingjie , Wu Ziyi , Chen Shangjie , Wang Min , Qu Hongdang 
Published: May 2022

Abstract
Background: In recent years, repeated transcranial magnetic stimulation (rTMS) has been used in clinical trials for the treatment of chronic pelvic pain syndrome, but its efficacy needs to be further verified.Objectives/Methods: This meta-analysis aimed to evaluate the efficacy and safety of transcranial magnetic stimulation in the treatment of chronic pelvic pain syndrome. Databases of PubMed, the Cochrane Library, Embase, CNKI, VIP, Wanfang, and SinoMed were searched for all clinical trials published before Nov.08.2021. The effect size was evaluated by using the standardized mean difference (SMD) and a 95% confidence interval (CI). Results: We included 7 studies comprising 99 participants. VAS scale was used as the main index of pain intensity, was observed (SMD=0.19, 95%CI -0.07 to 0.42, p=0.13) has no treatment effect. Reduction in depression ,anxiety and symptoms were not find out, there was a significant effect of quality of life (SF-36, SMD= -8.69, 95%CI -12.04 to -5.35, p＜0.00001). No serious adverse events were reported by the included articles.Conclusion: TMS may improve ADL, and long-term interventions of rTMS have a positive impact on pain and mood in patients with CPPS. However, these findings should be interpreted with caution due to the limitations of the included studies.
Clinical outcomes after single induction dose of etomidate versus ketamine for emergency department sepsis intubation: a randomized controlled trial
Authors: Srivilait Winchana , Bumrungphanithaworn Atidtaya , Daorattanachai Kiattichai , Limjindaporn Chitlada , Amnuaypattanapon Kumpol , Imsuwan Intanon , Diskumpon Nipon , Dasanadeba Ittabud , Siripakarn Yaowapha , Ueamsaranworakul Thosapol , Pornpanit Chatchanan , Pornpachara Vanussarin 
Published: May 2022

Abstract
Background: Patients with sepsis often require emergency intubation. In emergency departments (EDs), rapid-sequence intubation with a single-dose induction agent is standard practice but the best choice of induction agent in sepsis remains controversy. High quality RCTs are needed to determine the optimal induction agents in sepsis. Methods: We conducted a randomized, controlled, single-blind trial in the ED. We included septic patients who were aged at least 18 years and required sedation for emergency intubation. Patients were randomly assigned by a blocked randomization to receive 0.2-0.3 mg/kg of etomidate or 1-2 mg/kg of ketamine for intubation. Outcomes were compared with intention-to-treat analysis. The primary objective was to compare the 28-day survival outcomes between etomidate and ketamine. The secondary objectives outcomes were to compare 24-hour and 7-day survival rates, and adverse events after intubation. Results: 260 septic patients were enrolled; 130 patients/ drug arm whose baseline characteristics were well balance at baseline. In the etomidate group, 105 patients (80.8%) were alive at 28 days, as compared with 95 patients (73.1%) in the ketamine group (risk difference [RD], 7.7%; 95% confidence interval [CI], -2.5% to 17.9%; P=0.092). There was no significant difference in the proportion of patients who survived at 24 hours (91.5% vs. 96.2%; P=0.097) and survived at 7 days (87.7% vs. 87.7%; P=0.574). A significantly higher proportion of the etomidate group needed a vasopressor within 24 hours after intubation: 43.9% vs. 17.7%, RD, 26.2% (95% CI, 15.4% to 36.9%; P<0.001). Conclusions: In patients with sepsis who needed emergency intubation in the ED, there were no differences in early and late survival rates between etomidate and ketamine. However, etomidate associated with higher risks of early vasopressor needed after intubation. Trial registration: The trial protocol was registered in Thai Clinical Trials Registry (identification number; TCTR20210213001). Registered 13 February 2021 – Retrospectively registered, https://www.thaiclinicaltrials.org/export/pdf/TCTR20210213001.
Manganese exposure induces cellular aggregates and the accumulation of β-catenin in skin of zebrafish embryos
Authors: Gomes Geyse , Oliveira José Leonardo , Costa Manoel Luis , Mermelstein Claudia , Feitosa Natália Martins 
Published: May 2022

Abstract
The effects of manganese (Mn) toxicity in different organs and tissues in humans and other vertebrates have been studied since the beginning of the last century but most of its cellular effects remain largely unknown. Here we studied the effects of Mn in zebrafish, at the cellular level, due to the transparent nature of zebrafish larvae that enables a powerful analysis under the light microscope. The collection of our results shows that environmental concentrations of 0.5 mg/L affect swimbladder inflation; at concentration of 50 mg/L and 100 mg/L Mn (i) induces alterations in viability, swim bladder, heart, and size of zebrafish larvae, (ii) induces an increase in melanocyte area and the formation of cellular aggregates in the skin, and (iii) induces an accumulation of β-catenin in mesenchymal cells in the caudal fin of zebrafish larvae. Our data suggest that increased levels of Mn induce cell aggregate formation in the skin and the presence of more melanocytes in the zebrafish caudal fin. Interestingly, the adhesion protein β-catenin was activated in mesenchymal cells near the cell aggregates. These results open important new questions on the role of Mn toxicity on cellular organization and β-catenin responses in fishes.
The relationship between TEAD gene family and prognosis, tumor microenvironment and therapeutic target by pan-cancer analysis
Authors: Bai& Ruimin , Wu& Shaobo , Luo Ruiting , Wang Shengbang , Zhang Xiu , Song Liumei , Du Wenqian , He Ke , Ying Tingyi , Lei Hao , Zheng Yan 
Published: May 2022

Abstract
Background
Transcription Enhanced Association Domain (TEAD) transcription factors family involves in many signal transduction pathways, and is necessary for cell proliferation, apoptosis and other vital activities. TEAD1-4 is abnormally expressed in many tumors, and is related to tumor progression, drug resistance, poor prognosis, etc. In this study, we assessed the effect of the TEAD family on pan-cancer.
Methods
We downloaded transcriptome and clinical data from 33 types of TCGA tumor samples and adjacent tissues. Then, the expression characteristics of TEAD family members TEAD1, TEAD2, TEAD3 and TEAD4 in pan-cancer were analyzed. R packet and Cox regression were used to analyze the clinical correlation. In addition, the transcription level of TEAD and immune subtypes, stem cells, immune tumor microenvironment and drug sensitivity were analyzed by multidimensional correlation.
Results
We found the expression of TEAD1-4 was significantly different in various tumors. The survival time of patients with high and low TEAD1-4 gene expression was significantly different. TEAD1-4 was significantly associated with immune-infiltrating subtypes of tumor microenvironment (TME), and was positively correlated with interstitial cell infiltration and tumor cell dryness. The transcriptional expression level of TEAD1-4 was also related to drug response. TEAD may play a role in the sensitivity or resistance of tumor cells to drug therapy, and may serve as a therapeutic target to overcome drug-induced resistance.
Conclusions
Our systematic analysis reveals TEAD gene expression and its relationship with immune invasion, TME, tumor stem cells, drug sensitivity, and prognosis in cancer patients, and it is necessary to study the role of each TEAD family member in specific cancer type. Although further laboratory validation is needed, our study confirms that TEAD family genes are promising therapeutic targets and potential prognostic indicators in cancers.
Characteristics of leachate from refuse transfer stations in rural China
Authors: Xiang Huiming , Cheng Le , Liu Weiye , Wang Saier , Zhang Yi , Su Lianghu , Tan Chaoqun , Li Yuping 
Published: May 2022

Abstract
The properties of leachate from refuse transfer stations (RTSs) in rural China was indefinite. In this study, a total of 14 leachate samples from RTSs in nine provinces of China were characterized for their pH, electric conductivity, chromaticity, concentration of organic substances, nitrogen distribution, volatile organic compounds (VOCs), organic phosphorous pesticide, and heavy metals. The structural composition of chromophoric dissolved organic matter (CDOM) was also determined. To evaluate the leachate pollution potential in this study, a leachate pollution index (LPI) was derived and used. Chromium (Cr) was the most polluting heavy metal present in rural leachate. Ethanol and ethyl acetate were the most frequently detected VOCs at high concentrations. Three-dimensional fluorescence excitation-emission matrix spectra was used to characterize the CDOM. Three components, tryptophan (C1), tyrosine-like (C2), and humic and fulvic acid-like (C3) substances were identified from all 14 samples. Tryptophan was the major component of CDOM and present in 45.7% of the samples by calculating the fluorescence intensity percentage, on average. Pearson correlations revealed that the fluorescence intensity of C1 and C3 was strongly related to soluble chemical oxygen demand and dissolved oxygen carbon, while C2 had significant positive correlations with ammonia nitrogen and total phosphorus of the solid waste. This study provided detailed data and findings that could serve as a preliminary basis for broadening options for the treatment and management of leachate from rural RTSs in China.
Surgical outcomes of gastrectomy in hemodialysis patients with gastric cancer
Authors: Yoshimoto Toshiaki , Yoshikawa Kozo , Nakao Toshihiro , Tokunaga Takuya , Nishi Masaaki , Takasu Chie , Kashihara Hideya , Wada Yuma , Yamashita Shoko , Shimada Mitsuo 
Published: May 2022

Abstract

Background
Hemodialysis (HD) patients have an increased risk of postoperative complications and poor prognosis after surgery. Likewise, frailty, defined as decreased muscle strength and activity, affects prognosis after cancer surgery. We evaluated frailty as a prognostic factor in HD patients undergoing surgery for gastric cancer.
Methods
This study included eight HD patients who underwent radical surgery for gastric cancer (seven stage I, one stage II) from August 2009 to the present and 355 non-HD patients who underwent surgery for stage I or II gastric cancer from 2009 to 2018.
Results
Five-year overall survival (OS) was significantly lower in the HD group than in the control group (43% vs. 86%, respectively; P = .007). However, there was no significant difference in disease-free survival between groups; none of the HD patients died of gastric cancer. Three of the eight HD patients had frailty; those patients had significantly lower OS than the non-frail patients (0% vs. 67%; P = .045). HD, cancer stage, and modified Glasgow prognostic score were independent predictors of OS; HD correlated with the modified Glasgow prognostic score. No postoperative complications occurred among HD patients. Postoperative hospitalization did not significantly differ between groups. Six HD patients underwent reduction surgery with limited lymph node dissection.
Conclusion
No HD patient died of gastric cancer; however, OS was significantly lower among HD patients than among controls. Frailty was a strong prognostic factor among HD patients. Gastrectomy for HD patients is feasible; reduction surgery may be considered for frail patients.
Whole Genome DNA and RNA Sequencing of Whole Blood Elucidates the Genetic Architecture of Gene Expression Underlying a Wide Range of Diseases
Authors: Liu Chunyu , Joehanes Roby , Ma Jiantao , Wang Yuxuan , Sun Xianbang , Keshawarz Amena , Sooda Meera , Huan Tianxiao , Hwang Shih-Jen , Bui Helena , Tejada Brandon , Munson Peter J. , Cumhur Demirkale , Heard-Costa Nancy L. , Pitsillides Achilleas N , Peloso Gina M. , Feolo Michael , Sharopova Nataliya , Vasan Ramachandran S. , Levy Daniel 
Published: May 2022

Abstract
To create a scientific resource of expression quantitative trail loci (eQTL), we conducted a genome-wide association study (GWAS) using genotypes obtained from whole genome sequencing (WGS) of DNA and gene expression levels from RNA sequencing (RNA-seq) of whole blood in 2622 participants in Framingham Heart Study. We identified 6,778,286 cis-eQTL variant-gene transcript (eGene) pairs at p < 5x10− 8 (2,855,111 unique cis-eQTL variants and 15,982 unique eGenes) and 1,469,754 trans-eQTL variant-eGene pairs at p < 1e-12 (526,056 unique trans-eQTL variants and 7,233 unique eGenes). In addition, 442,379 cis-eQTL variants were associated with expression of 1518 long non-protein coding RNAs (lncRNAs). Gene Ontology (GO) analyses revealed that the top GO terms for cis-eGenes are enriched for immune functions (FDR < 0.05). The cis-eQTL variants are enriched for SNPs reported to be associated with 815 traits in prior GWAS, including cardiovascular disease risk factors. As proof of concept, we used this eQTL resource in conjunction with genetic variants from public GWAS databases in causal inference testing (e.g., COVID-19 severity). After Bonferroni correction, Mendelian randomization analyses identified putative causal associations of 60 eGenes with systolic blood pressure, 13 genes with coronary artery disease, and seven genes with COVID-19 severity. This study created a comprehensive eQTL resource via BioData Catalyst that will be made available to the scientific community. This will advance understanding of the genetic architecture of gene expression underlying a wide range of diseases.
The relationship between high absolute lymphocyte counts and favorable prognosis in eribulin therapy is seen in first-line chemotherapy for metastatic breast cancer: Combined analysis of two phase 2 studies
Authors: Kimura Kosei , Takashima Tsutomu , Oku Hiroyo , Ikari Ayana , Tominaga Tomo , Takai Saki , Sakane Junna , Tanaka Michiaki , Kawajiri Hidemi , Kashiwagi Shinichiro , Tokunaga Shinya , Nishimura Shigehiko , Noda Satoru , Iwamoto Mitsuhiko 
Published: May 2022

Abstract
Purpose: While the absolute lymphocyte count (ALC) and neutrophil-to-lymphocyte ratio (NLR) are associated with prolonged progression-free survival (PFS) and overall survival (OS), the influence of previous chemotherapy on blood cell counts may necessitate an evaluation of baseline ALC and NLR in patients receiving first-line chemotherapy. Methods: Patients with human epidermal growth factor receptor 2 (HER2)-negative metastatic breast cancer (MBC) who received first-line eribulin chemotherapy in two phase 2 trials (BIRICHEN and OMC-BC 03) were retrospectively analyzed. HER2-negative MBC patients who received first-line chemotherapy other than eribulin (treatment of physician’s choice; TPC) at the Osaka Medical and Pharmaceutical University Hospital between March 2013 and March 2017 were also analyzed for comparison. Results: In the eribulin group, the median OS (mOS) was 30.9 and 17.8 months in the high-(H-)ALC (≥1500/μL; n=33) and low-(L-)ALC (<1500/μL; n=26) groups, respectively (hazard ratio [HR], 0.52; 95% confidence interval [CI]: 0.27-1.01), whereas it was 30.9 months and 15.4 months in the L-NLR (<2.5; n=23) and H-NLR (≥2.5; n = 36) groups, respectively (HR, 0.49; 95% CI: 0.25-0.95). In the TPC group, neither ALC nor NLR was associated with OS or PFS extension. After propensity-score matching, the mOS in the eribulin group was 32.0 and 19.6 months, respectively, in the H- and L-ALC groups (HR, 0.43; 95% CI: 0.18-0.99), while OS in the L- and H-NLR groups showed no significant differences in the eribulin group (HR, 0.65; 95% CI: 0.27-1.58). Conclusions: ALC is a prognostic marker for first-line eribulin chemotherapy, but not for other agents.
Lower expression of NINJ1 (Ninjurin 1), a mediator of plasma membrane rupture, is associated with advanced disease and worse prognosis in serous ovarian cancer
Authors: BERKEL CAGLAR , CACAN Ercan 
Published: May 2022

Abstract
Gasdermin proteins (GSDMs) form pores in cell membranes upon various stimuli, leading to the release of certain proinflammatory molecules such as IL-1β and IL-18, and this ultimately results in pyroptotic cell death. NINJ1 has recently been identified as a cell membrane protein responsible for final complete plasma membrane rupture following lytic cell death mechanisms including pyroptosis, causing the release of relatively larger molecules such as HMGB1 and LDH. In this study, we reported higher GSDMD and lower GSDME protein levels in ovarian tumors compared to surrounding non-malignant stroma. We found that NINJ1 expression decreases from early to late stage in serous ovarian cancer, and the percentage of NINJ1 copy number loss events is the highest in ovarian cancer among other cancers. Moreover, we showed that low expression of NINJ1 is associated with shorter overall survival of patients with ovarian cancer. In support of the findings showing that low NINJ1 expression contributes to worse prognosis in this most lethal gynecological malignancy, NINJ1 expression was found to be lower in cisplatin-resistant cells compared to cisplatin-sensitive ovarian cancer cells in vitro. We suggest that the members of gasdermin family might have distinct functions in serous ovarian cancer, and low levels of NINJ1 might contribute, at least in part, to the progression and poorer prognosis of ovarian cancer. A complete picture of how pyroptosis and subsequent plasma membrane rupture are involved in ovarian cancer will be of high importance in order to identify actionable therapeutic vulnerabilities within this newly identified group of proteins.