bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemical Synthesis of Shape‐controlled Cu−Ni Nanocomposite and its Application for Nonenzymatic Glucose Sensing at Nanomolar Level
Authors: Pang Yuanhao , Xiao Zhourui , Deng Yanan , Zhou Xueying , Wang Yu , Yuan Yali , Zhang Yun 
Published: October 2022

AbstractThe electrodeposition method was firstly applied to obtain uniform cube‐shaped copper nanoparticles on conductive glass (ITO), and then a layer of tiny nickel nanoparticles. A bimetallic composite electrode (Cu−Ni/ITO), characterized by TEM, XPS and XRD, was prepared to construct the non‐enzyme electrochemical glucose sensor with high catalytic activity. The catalytic performance of Cu−Ni/ITO had been greatly improved, probably due to the synergistic bimetallic catalysis effect. The electrode had a satisfactory linear response in the range of 2.5×10−7 M to 2.6×10−3 M, with detection limit as low as 67 nM. Besides, Cu−Ni/ITO had good anti‐interference ability and reproducibility, indicating the promising application for glucose detection in practical samples.
An Improved Model for Circular RNA Overexpression: Using the Actin Intron Reveals High Circularization Efficiency
Authors: Li Feiya , Lyu Juanjuan , Yang Yang , Yang Qiwei , Santos Cristian , Yang Burton B. 
Published: October 2022

AbstractTraditionally, the group 1 intron of the T4 td gene is used to generate a foreign circular sequence. However, the T4 system has been shown to be fairly inefficient in expressing circular RNA (circRNA). Here, a new method is developed to express circular sequences with high circularization efficiency to strengthen the confidence for future circRNA functional studies. CircRNA expression plasmids, constructed with different lengths derived from the actin intron (15‐nt, 30‐nt, 60‐nt, 100‐nt, 180‐nt) and T4 intron, are introduced into human and mouse cell lines 293T and B16. Junction detection and sequencing are used to determine successful circularization of introns and their expression efficiencies. An actin intron with a medium length (60‐nt–100‐nt) shows significantly increased efficiency of circularization, whereas intron‐100‐nt shows the best efficiency in most conditions. RNA pull‐down assays are designed to precipitate the splicing factors that are bound to the introns and intron/exon junction. The precipitated proteins are analyzed by mass spectrometry (MS), aiming to identify the possible underlying mechanism behind the high circularization efficiency. This expression system has been validated using different circRNAs, and such method shows potential in contributing to the expanding field of circRNA studies.
Heat and mass transfer of MHD Jeffrey nanofluid flow through a porous media past an inclined plate with chemical reaction, radiation, and Soret effects
Authors: Haritha A. , Vishali B. , Venkata Lakshmi C. 
Published: October 2022

AbstractThis paper investigates the heat and mass transfer of an unsteady, magnetohydrodynamic incompressible water‐based nanofluid (Cu and TiO2) flow over a stretching sheet in a transverse magnetic field with thermal radiation Soret effects in the presence of heat source and chemical reaction. The governing differential equations are transformed into a set of nonlinear ordinary differential equations and solved using a regular perturbation technique with appropriate boundary conditions for various physical parameters. The effects of different physical parameters on the dimensionless velocity, temperature, and concentration profiles are depicted graphically and analyzed in detail. Finally, numerical values of the physical quantities, such as the local skin‐friction coefficient, the Nusselt number, and the Sherwood number, are presented in tabular form. It is concluded that the resultant velocity reduces with increasing Jeffrey parameter and magnetic field parameter. Results describe that the velocity and temperature diminish with enhancing the thermal radiation. Both velocity and concentration are enhanced with increases of the Soret parameter. Also, it is noticed that the solutal boundary layer thickness decreases with an increase in chemical reaction parameters. This is because chemical molecular diffusivity reduces for higher values of chemical reaction parameter. Also, water‐based TiO2 nanofluids possess higher velocity than water‐based Cu nanofluids. Comparisons with previously published work performed and the results are found to be in excellent agreement. This fluid flow model has several industrial applications in the field of chemical, polymer, medical science, and so forth.
Dual solution of heat transfer in hydromagnetic micropolar fluid flow over a stretching sheet in a porous media: A numerical approach
Authors: Hussain Mayzul A. , Ahmed Sahin 
Published: October 2022

AbstractIn this study, the researcher looks at the heat transmission of an incompressible magnetohydrodynamics micropolar fluid across a moving stretched surface in a Darcian permeable medium. The proper boundary conditions are used to facilitate the numerical solution (bvp4c) of the transformed governing equations. Graphical discussions have been made of the influence of the physical parameters on the velocity, angular velocity (microrotation), and temperature, and the distributions are accentuated on the plots via MATLAB. The study is validated by the previous work and it is found appropriate for investigation, where the absolute difference between the previous work and the present investigation by adopting the finite difference scheme is smaller than which implies that the scheme is stable and convergent. The microrotation has a great impact on the micropolar fluid with the influences of buoyancy forces, source, and suction over the stretching surface in a Darcian regime. With a rise in the heat source parameter, both velocity and microrotational profiles lessen, but the opposite is true for temperature. Eringen number () rises with the flow velocity, whereas temperature and microrotational profiles show the reverse relationship. The current study focused on particular applications in non‐Newtonian fluid mechanics, polymer flows in filtration systems and metallurgical procedures that included cooling unbroken strips or filaments via a static fluid.
The Economic Burden of Severe Osteoporotic Fractures in the French Healthcare Database: The FRACTOS Study
Authors: Thomas Thierry , Tubach Florence , Bizouard Geoffray , Crochard Anne , Maurel Frédérique , Perrin Laure , Collin Cédric , Roux Christian , Paccou Julien 
Published: October 2022

ABSTRACT
Osteoporosis carries a high medical, economic, and societal burden principally because of the risk of severe fractures. The objective of this cost-of-illness study was to describe health resource utilization and associated costs in all patients aged ≥50 years hospitalized for a severe osteoporotic fracture over a 6-year period (2009 to 2014) in France. Data were extracted from the French national healthcare database (SNDS) on all health care resource utilization between the index date (date of hospitalization for first fracture during the enrollment period) and study end (December 31, 2016) or until the patient died. Costing was restricted to direct costs and determined from the payer perspective. Variables related to costs were identified through multivariate logistic regression analysis. A total of 356,895 patients were included (median follow-up 39.1 months). In the year after the index fracture, 36,622 patients (10.5%) were rehospitalized for a fracture-related reason. Only 18,474 (5.3%) underwent bone densitometry and 58,220 (16.7%) received a specific treatment. The total annual per capita osteoporosis-related cost in the year after the index severe osteoporotic fracture was €18,040 (from €8598 for multiple ribs to €21,085 for hip fracture) of which €17,905 was incurred by fracture-related costs. The cost incurred by management of osteoporosis was €135. Over years 2 to 5, the mean annual per capita costs of fracture treatment (€806, mostly attributable to the treatment of refractures) continued to dominate those of osteoporosis management (€99). Total annual cost of care was €1260 million (year 2014). Variables associated with higher cost were older age, male sex, site of fracture, a history of prior osteoporotic fracture, and the number of refracture events. The 5-year cost of severe osteoporotic fractures to the French health care system is high and mostly attributable to the treatment of refractures. Improved fracture prevention measures in patients with osteoporosis is crucial to reduce the economic burden of the disease. © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
Muscle mass, muscle strength and mortality in kidney transplant recipients: results of the TransplantLines Biobank and Cohort Study
Authors: van Vliet Iris M.Y. , Post Adrian , Kremer Daan , Boslooper‐Meulenbelt Karin , van der Veen Yvonne , de Jong Margriet F.C. , Pol Robert A. ,   TransplantLines Investigators, Jager‐Wittenaar Harriët , Navis Gerjan J. , Bakker Stephan J.L. 
Published: October 2022

AbstractBackgroundSurvival of kidney transplant recipients (KTR) is low compared with the general population. Low muscle mass and muscle strength may contribute to lower survival, but practical measures of muscle status suitable for routine care have not been evaluated for their association with long‐term survival and their relation with each other in a large cohort of KTR.MethodsData of outpatient KTR ≥ 1 year post‐transplantation, included in the TransplantLines Biobank and Cohort Study (ClinicalTrials.gov Identifier: NCT03272841), were used. Muscle mass was determined as appendicular skeletal muscle mass indexed for height2 (ASMI) through bio‐electrical impedance analysis (BIA), and by 24‐h urinary creatinine excretion rate indexed for height2 (CERI). Muscle strength was determined by hand grip strength indexed for height2 (HGSI). Secondary analyses were performed using parameters not indexed for height2. Cox proportional hazards models were used to investigate the associations between muscle mass and muscle strength and all‐cause mortality, both in univariable and multivariable models with adjustment for potential confounders, including age, sex, body mass index (BMI), estimated glomerular filtration rate (eGFR) and proteinuria.ResultsWe included 741 KTR (62% male, age 55 ± 13 years, BMI 27.3 ± 4.6 kg/m2), of which 62 (8%) died during a median [interquartile range] follow‐up of 3.0 [2.3–5.7] years. Compared with patients who survived, patients who died had similar ASMI (7.0 ± 1.0 vs. 7.0 ± 1.0 kg/m2; P = 0.57), lower CERI (4.2 ± 1.1 vs. 3.5 ± 0.9 mmol/24 h/m2; P < 0.001) and lower HGSI (12.6 ± 3.3 vs. 10.4 ± 2.8 kg/m2; P < 0.001). We observed no association between ASMI and all‐cause mortality (HR 0.93 per SD increase; 95% confidence interval [CI] [0.72, 1.19]; P = 0.54), whereas CERI and HGSI were significantly associated with mortality, independent of potential confounders (HR 0.57 per SD increase; 95% CI [0.44, 0.81]; P = 0.002 and HR 0.47 per SD increase; 95% CI [0.33, 0.68]; P < 0.001, respectively), and associations of CERI and HGSI with mortality remained independent of each other (HR 0.68 per SD increase; 95% CI [0.47, 0.98]; P = 0.04 and HR 0.53 per SD increase; 95% CI [0.36, 0.76]; P = 0.001, respectively). Similar associations were found for unindexed parameters.ConclusionsHigher muscle mass assessed by creatinine excretion rate and higher muscle strength assessed by hand grip strength are complementary in their association with lower risk of all‐cause mortality in KTR. Muscle mass assessed by BIA is not associated with mortality. Routine assessment using both 24‐h urine samples and hand grip strength is recommended, to potentially target interdisciplinary interventions for KTR at risk for poor survival to improve muscle status.
Dynamic contrast‐enhanced MRI and Doppler sonography in patients with squamous cell carcinoma of head and neck treated with induction chemotherapy
Authors: Chawla Sanjeev , Loevner Laurie , Mohan Suyash , Lin Alexander , Sehgal Chandra M. , Poptani Harish 
Published: October 2022

AbstractIn view of the inherent limitations associated with performing dynamic contrast enhanced‐magnetic resonance imaging (DCE‐MRI) in clinical settings, current study was designed to provide a proof of principle that Doppler sonography and DCE‐MRI derived perfusion parameters yield similar hemodynamic information from metastatic lymph nodes in squamous cell carcinomas of head and neck (HNSCCs). Strong positive correlations between volume fraction of plasma space in tissues (Vp) and blood volume (r = 0.72, p = 0.02) and between Vp and %area perfused (r = 0.65, p = 0.04) were observed. Additionally, a moderate positive correlation trending towards significance was obtained between volume transfer constant (Ktrans) and %area perfused (r = 0.49, p = 0.09).
Warmer incubation temperatures and later lay‐orders lead to shorter telomere lengths in wood duck (Aix sponsa) ducklings
Authors: Hope Sydney F. , Angelier Frédéric , Ribout Cécile , Groffen Jordy , Kennamer Robert A. , Hopkins William A. 
Published: October 2022

AbstractThe environment that animals experience during development shapes phenotypic expression. In birds, two important aspects of the early‐developmental environment are lay‐order sequence and incubation. Later‐laid eggs tend to produce weaker offspring, sometimes with compensatory mechanisms to accelerate their growth rate to catch‐up to their siblings. Further, small decreases in incubation temperature slow down embryonic growth rates and lead to wide‐ranging negative effects on many posthatch traits. Recently, telomeres, noncoding DNA sequences at the end of chromosomes, have been recognized as a potential proxy for fitness because longer telomeres are positively related to lifespan and individual quality in many animals, including birds. Although telomeres appear to be mechanistically linked to growth rate, little is known about how incubation temperature and lay‐order may influence telomere length. We incubated wood duck (Aix sponsa) eggs at two ecologically‐relevant temperatures (34.9°C and 36.2°C) and measured telomere length at hatch and 1 week after. We found that ducklings incubated at the lower temperature had longer telomeres than those incubated at the higher temperature both at hatch and 1 week later. Further, we found that later‐laid eggs produced ducklings with shorter telomeres than those laid early in the lay‐sequence, although lay‐order was not related to embryonic developmental rate. This study contributes to our broader understanding of how parental effects can affect telomere length early in life. More work is needed to determine if these effects on telomere length persist until adulthood, and if they are associated with effects on fitness in this precocial species.
Role of low‐intensity pulsed ultrasound in regulating macrophage polarization to accelerate tendon–bone interface repair
Authors: Xu Zihan , Li Shengcan , Wan Liyang , Hu Jianzhong , Lu Hongbin , Zhang Tao 
Published: October 2022

AbstractLow‐intensity pulsed ultrasound (LIPUS) has been proven to accelerate the healing of the tendon–bone interface (TBI), and macrophages are considered to play an important regulatory role. This study was designed to explore the polarization of macrophages during treatment of TBI injury with LIPUS. In a rat model of rotator cuff tear, LIPUS or mock sonication (controls) was administered from 1 week postoperatively. The supraspinatus–supraspinatus tendon‐humerus complexes were harvested for further evaluation at different time points for measures such as new bone formation, TBI maturity, ultimate failure load and stiffness, and types of macrophages. In vitro, bone marrow‐derived macrophages were cultured, and polarization was identified after stimulation with or without LIPUS (the LIPUS or control groups, respectively). Two weeks posttreatment, the LIPUS group showed higher bone volume/total volume ratios and better TBI maturity scores. Six weeks posttreatment, the failure load of the LIPUS group was significantly higher than that of the control group. LIPUS also accelerated initial inflammatory macrophage accumulation and facilitated anti‐inflammatory macrophage polarization (M2) in the late period. In the in vitro macrophage polarization model, the LIPUS group showed a higher proportion of M2 macrophages and mRNA expression of anti‐inflammatory genes than the control group, while there was no significant difference in the proinflammatory macrophages between the two groups. Our observations revealed that macrophage polarization may be a potential mechanism of LIPUS treatment for TBI repair.
Longitudinal neuroplasticity after ankle sprain in mice: A voxel‐based morphometry study on 11.7T MRI
Authors: Xue Xiao'ao , Zhang Yuwen , Tao Weichu , Wei Lei , Li Qianru , Ma Tengjia , Xu Xiaoyun , Wang Yiran , Gu Xicheng , Xu Zhangran , Wang He , Hua Yinghui 
Published: October 2022

AbstractLateral ankle sprains (LAS) might lead to joint sensory deafferentation, which induces maladaptive neuroplasticity, especially the morphological atrophy of the cerebellar vermis. However, longitudinal evidence on the causality of injury and neural differences is still lacking. To this end, this study aimed to determine whether the morphology of the central nervous system would be altered before and after ligament transection in LAS mouse models. A total of 40 C57BL/6 mice were randomly divided among the LAS, Sham and Blank groups. We repeatedly performed the balance beam test and neural voxel‐based morphometry (VBM) measurements using an 11.7 T magnetic resonance imaging before and 2 months after the surgery. The results showed that for balance outcomes, the LAS group had a significantly longer time and more slips of the balance beam tests compared with the Sham and Blank groups at 2 months after surgery, with no significant difference among the three groups before surgery. Regarding the VBM analysis, the LAS group showed significantly lower VBM values in the central lobule III of the cerebellar vermis and medial amygdalar nucleus (MEA) compared with the Sham and Blank groups after surgery, with no significant difference among the three groups before surgery. In conclusion, lateral ligament injuries might lead to morphological atrophy of the cerebellar vermis in animal models, which might pave the way for the pathological process of ankle instability after LAS.
Development and trial of an instrument to evaluate accredited pharmacists' clinical home medicines review reports in Australia
Authors: Patounas Marea , Lau Esther T. L. , Rigby Deborah , Chan Vincent , Nissen Lisa M. 
Published: October 2022

AbstractIn Australia, clinical reports are written by an accredited pharmacist following in‐home patient consultations as part of a home medicines review (HMR). These reports communicate clinical findings and recommendations to the patient's general practitioner to optimise medicines and improve patient health. However, it is unknown if clinical HMR reports adhere to practice guidelines. This study aimed to develop an instrument from Australian practice guidelines, and then test the instrument by evaluating a small sample of clinical HMR reports written by accredited pharmacists. An instrument was developed from a consolidation of HMR practice guidelines and then applied to a small sample of de‐identified clinical HMR reports provided by accredited pharmacists. The instrument developed contained 30 criteria for clinical HMR report writing, and 20 HMR reports were evaluated from 12 accredited pharmacists. Seven of the 30 criteria were met by all clinical HMR reports evaluated (were consumer‐focused, documented a medicines list, medicines strengths, medicines directions, medication‐related problems, and included both evidence‐based and clinical recommendations for optimising medicines management). However, of the 20 HMR reports evaluated only 30% (n = 6) documented the general practitioner's reason for HMR referral, 60% (n = 12) detailed allergies/adverse drug reactions, 50% (n = 10) documented an adherence statement, and 20% (n = 4) documented vaccination status. Clinical HMR reports evaluated in this small study were aligned with practice guidelines for some criteria. Future research is warranted in a larger study to further investigate clinical HMR report writing adherence to practice guidelines in Australia.
Late Pleniglacial – Late Glacial climate oscillations detected in the organic lacustrine succession at the Lipowo site, north‐eastern Poland
Authors: Rychel Joanna , Sokołowski Robert J. , Sieradz Dominika , Hrynowiecka Anna , Mirosław‐Grabowska Joanna , Sienkiewicz Elwira , Niska Monika , Szymanek Marcin , Zbucki Łukasz , Ciołko Urszula , Rogóż‐Matyszczak Anna 
Published: October 2022

AbstractThe profile of the Lipowo palaeolake (NE Poland) preserves a record of environmental and climate changes from the Last Glacial Termination (16.5k cal ka BP), stretching from the Oldest Dryas to the youngest Allerød warm oscillation. In this interdisciplinary study of the lacustrine succession at Lipowo, we used biological and geochemical indicators and multiproxy analyses to reconstruct environmental changes in the lake ecosystem, such as mollusc, zooplankton and vegetation development, trophic state, water temperature and water level. Four cold and four warm climate oscillations of different durations were identified on this basis: the Older Dryas climate deterioration and two short‐term coolings within the Allerød Interstadial, separated by warm periods in the Bølling and Allerød. The covering of the lake series by diamicton deposits from the melting of dead‐ice blocks is a particularly interesting and rare phenomenon for a postglacial area. The hydrological system of the catchment was generally closed, with a periodically opening and existing flow‐through lake. Over the period of the lake's existence, vegetation changed in general from steppe tundra to forest with birch and pine. For most of this time, the palaeolake was mainly oligotrophic, with increased productivity and a higher trophic level only notable during the middle Bølling.
Emerging Concepts in Iodine Transfer Polymerization
Authors: Kumru Baris , Antonietti Markus 
Published: October 2022

AbstractControlled radical polymerization corresponds to variety of synthetic strategies that aim the generation of precise macromolecular architectures. Iodine transfer polymerization (ITP) is one of the oldest methods to conduct controlled radical polymerization; however, it seems to have lost visibility compared to other popular techniques. It relies on utilization of iodine species in reversible deactivation kinetics, and it has some significant advantages compared to other methods. Its simplicity, minimized toxicity, metal‐free nature, no coloring on final product, and ease of purification provide widespread applicability, even in industry. In this perspective, the basics of ITP are re‐introduced, and emerging technologies (heterophase polymerization, photoinitiation, and sustainability) in ITP are discussed.