bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prior Exposure to Azithromycin and Azithromycin Resistance Among Persons Diagnosed With Neisseria gonorrhoeae Infection at a Sexual Health Clinic: 2012–2019
Authors: Rowlinson Emily , Soge Olusegun O , Hughes James P , Berzkalns Anna , Thibault Christina , Kerani Roxanne P , Khosropour Christine M , Manhart Lisa E , Golden Matthew R , Barbee Lindley A 
Published: August 2022

Abstract

Background
There is conflicting evidence on whether prior azithromycin (AZM) exposure is associated with reduced susceptibility to AZM (AZMRS) among persons infected with Neisseria gonorrhoeae (NG).

Methods
The study population included Public Health–Seattle and King County Sexual Health Clinic (SHC) patients with culture-positive NG infection at ≥1 anatomic site whose isolates were tested for AZM susceptibility in 2012–2019. We used multivariate logistic regression to examine the association of time since last AZM prescription from the SHC in ≤12 months with subsequent diagnosis with AZMRS NG (minimum inhibitory concentration [MIC], ≥2.0 µg/mL) and used linear regression to assess the association between the number of AZM prescriptions in ≤12 months and AZM MIC level, controlling for demographic, behavioral, and clinical characteristics.

Results
A total of 2155 unique patients had 2828 incident NG infections, 156 (6%) of which were caused by AZMRS NG. AZMRS NG was strongly associated with receipt of AZM from the SHC in the prior 29 days (adjusted odds ratio, 6.76; 95% confidence interval [CI], 1.76 to 25.90) but not with receipt of AZM in the prior 30–365 days. Log AZM MIC level was not associated with the number of AZM prescriptions within ≤12 months (adjusted correlation, 0.0004; 95% CI, –.04 to .037) but was associated with number of prescriptions within &lt;30 days (adjusted coefficient, 0.56; 95% CI, .13 to .98).

Conclusions
Recent individual-level AZM treatment is associated with subsequent AZMRS gonococcal infections. The long half-life and persistence of subtherapeutic levels of AZM may result in selection of resistant NG strains in persons with recent AZM use.
American Primacy and US–China Relations: The Cold War Analogy Reversed
Authors: Harris Peter , Marinova Iren 
Published: August 2022

Abstract
Does today’s US–China relationship resemble the bygone rivalry between the United States and the Soviet Union? In this article, we suggest that there are some instructive similarities between US–China and US–Soviet relations, but the Cold War analogy works best when the contemporary United States is cast in the role of the historical Soviet Union. Specifically, the United States (1991–present) has in common with the Soviet Union (1945–91) the fact that it occupies a position of near dominance on the Eurasian continent during a prolonged era of relative peace. It was Soviet hegemony in Eastern Europe that gave the bipolar international system of the Cold War era its defining characteristic—that is, a geographic distribution of power assets in Eurasia that the United States and its allies mobilized to resist and overturn. Now, it is US primacy in Eurasia that serves to define the basic contours of the present (if ailing) unipolar international system: a geopolitical configuration that denies any power other than the United States the opportunity to carve out an effective sphere of influence. In short, the legacy forward deployment of US forces shapes the context within which Sino-American relations are unfolding just as the legacy occupation of Eastern Europe by the Soviet Union structured superpower relations during the Cold War.
An Exploratory Study of How Teacher Type and Experience Relate to Perceptions of Adding More Recess during the School Day
Authors: Erwin Heather , Thornton Michelle 
Published: August 2022

Abstract
The primary purpose of this study was to explore elementary faculty perceptions of adding more recess during the school day. Secondarily, authors compared perceptions by teacher type and years of experience. Participants were 25 teachers (representing all grade levels and areas) from one elementary school in the southeastern region of the United States. Participants completed a questionnaire regarding their perceptions toward recess before/after implementing an additional recess period. No significant differences appeared when analyzing data from all participants. However, when examining data by teacher type and experience, a negative statistical significance was found. A school policy of two recess periods per day was perceived less positively by elementary faculty after implementation, and teachers with more experience viewed it more negatively than teachers with less experience. Grade-level teachers were less enthusiastic toward the inclusion of more recess time than administrators, aides, and special area teachers. As school personnel make shifts in school policy related to recess offerings, professional development on the positive outcomes can be geared toward more experienced and grade-level teachers.
Corticosteroid-Sparing Effects of Filgotinib in Moderately to Severely Active Ulcerative Colitis: Data from the Phase 2b/3 SELECTION Study
Authors: Loftus Edward V , Vermeire Séverine , Feagan Brian G , Le Brun Franck-Olivier , Oortwijn Alessandra , Moerch Ulrik , Sandborn William J , Hibi Toshifumi 
Published: August 2022

Abstract

Background and Aims
Corticosteroid-free remission is an important treatment goal for patients with ulcerative colitis [UC]. The corticosteroid-sparing effects of filgotinib, an oral, Janus kinase 1 preferential inhibitor, were assessed in SELECTION, a placebo-controlled, phase 2b/3 trial in moderately to severely active UC.

Methods
These post hoc analyses assessed 1-, 3-, 6-, and 8-month rates of corticosteroid-free clinical remission at Week 58 and change in median daily prednisone-equivalent dose over time. A matching-adjusted indirect comparison [MAIC] of maintenance studies assessed corticosteroid-free remission with filgotinib 200 mg, intravenous vedolizumab, subcutaneous vedolizumab, and oral tofacitinib.

Results
The Maintenance Study full analysis set included 199 patients receiving filgotinib 200 mg and 98 receiving placebo. Among patients receiving corticosteroids at Maintenance Study baseline, at Week 58, 30.4%, 29.3%, 27.2%, and 21.7% receiving filgotinib had been in corticosteroid-free remission for ≥1, ≥3, ≥6, or ≥8 months, respectively, versus 6.4% receiving placebo across thresholds [p &lt;0.05]. Median daily prednisone-equivalent dose decreased from 17.5 mg/day to 10.0 mg/day with filgotinib treatment during the Maintenance Study. Based upon the MAIC, filgotinib was associated with greater likelihood of corticosteroid-free clinical remission versus intravenous vedolizumab (odds ratio [OR], 15.2; 95% confidence interval [CI], 1.6–139.9; p &lt;0.05]) and similar odds to subcutaneous vedolizumab [OR, 3.8; CI, 0.2–63.8; p = 0.36] in biologic-naïve patients, and similar odds to tofacitinib overall [OR, 2.0; 0.4–9.1; p = 0.39].

Conclusions
Filgotinib 200 mg demonstrated corticosteroid-sparing effects and maintained corticosteroid-free clinical remission in patients with UC. MAIC results should be interpreted cautiously given the large CIs and differences in study design and patient populations. [ClinicalTrials.gov: NCT02914522].
AFA-Recur: an ESC EORP AFA-LT registry machine-learning web calculator predicting atrial fibrillation recurrence after ablation
Authors: Saglietto Andrea , Gaita Fiorenzo , Blomstrom-Lundqvist Carina , Arbelo Elena , Dagres Nikolaos , Brugada Josep , Maggioni Aldo Pietro , Tavazzi Luigi , Kautzner Josef , De Ferrari Gaetano Maria , Anselmino Matteo 
Published: August 2022

AbstractAimsAtrial fibrillation (AF) recurrence during the first year after catheter ablation remains common. Patient-specific prediction of arrhythmic recurrence would improve patient selection, and, potentially, avoid futile interventions. Available prediction algorithms, however, achieve unsatisfactory performance. Aim of the present study was to derive from ESC-EHRA Atrial Fibrillation Ablation Long-Term Registry (AFA-LT) a machine-learning scoring system based on pre-procedural, easily accessible clinical variables to predict the probability of 1-year arrhythmic recurrence after catheter ablation.Methods and resultsPatients were randomly split into a training (80%) and a testing cohort (20%). Four different supervised machine-learning models (decision tree, random forest, AdaBoost, and k-nearest neighbour) were developed on the training cohort and hyperparameters were tuned using 10-fold cross validation. The model with the best discriminative performance on the testing cohort (area under the curve—AUC) was selected and underwent further optimization, including re-calibration. A total of 3128 patients were included. The random forest model showed the best performance on the testing cohort; a 19-variable version achieved good discriminative performance [AUC 0.721, 95% confidence interval (CI) 0.680–0.764], outperforming existing scores (e.g. APPLE score: AUC 0.557, 95% CI 0.506–0.607). Platt scaling was used to calibrate the model. The final calibrated model was implemented in a web calculator, freely available at http://afarec.hpc4ai.unito.it/.ConclusionAFA-Recur, a machine-learning-based probability score predicting 1-year risk of recurrent atrial arrhythmia after AF ablation, achieved good predictive performance, significantly better than currently available tools. The calculator, freely available online, allows patient-specific predictions, favouring tailored therapeutic approaches for the individual patient.
Study on the death and disease burden caused by high sugar-sweetened beverages intake in China from 1990 to 2019
Authors: Jiang Yingying , Xu Tingling , Dong Wenlan , Chu Cordia , Zhou Maigeng 
Published: August 2022

Abstract

Background
To analysis the death and disease burden caused by high sugar-sweetened beverages intake in China from 1990 to 2019.

Methods
Data were obtained from the 2019 Global Burden of Disease Study. We analyzed the death cases caused by high sugar-sweetened beverages intake in China and provinces from 1990 to 2019, as well as the disease burden (including disability-adjusted of life year), years of life lost and years lived with disability, and compared the changes of death in 1990 and 2019.

Results
In 2019, the number of deaths attributed to sugar-sweetened beverages in China reached 46 633 with an increase of 95% compared with 1990. The proportion of deaths caused by excessive consumption of carbon-containing beverages increased from 0.34% in 1990 to 0.46% in 2019, an increase of 35%. In 2019, the top five provinces in China with more deaths caused by excessive intake of sugary beverages were Shandong, Henan, Hebei, Hunan and Guangdong, with the number of death cases 4337, 3881, 3010, 2762 and 2611, respectively.

Conclusions
The number of deaths and disease burdens caused by high sugar-sweetened beverages intake in China has increased significantly over the past three decades. The burden of disease due to high intake of sugary beverages varies widely from province to province. We suggest that attention should be paid to the problem of excessive intake of high sugar-sweetened beverages for Chinese population. In addition to regular monitoring and investigation of sugar-sweetened beverage intake, comprehensive measures should be taken in China’s sugar control work.
Gene targeting of dikaryotic Pleurotus ostreatus nuclei using the CRISPR/Cas9 system
Authors: Yamasaki Fuga , Nakazawa Takehito , Oh Minji , Bao Dapeng , Kawauchi Moriyuki , Sakamoto Masahiro , Honda Yoichi 
Published: August 2022

ABSTRACT
Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-assisted gene targeting is a promising method used in molecular breeding. We recently reported the successful introduction of this method in the monokaryotic Pleurotus ostreatus (oyster mushroom), PC9. However, considering their application in mushroom breeding, dikaryotic strains (with targeted gene mutations in both nuclei) need to be generated. This is laborious and time-consuming because a classical crossing technique is used. Herein, we report a technique that targets both nuclei of dikaryotic P. ostreatus, PC9×#64 in a transformation experiment using plasmid-based CRISPR/Cas9, with the aim of developing a method for efficient and rapid molecular breeding. As an example, we targeted strains with low basidiospore production ability through the meiosis-related genes mer3 or msh4. Four different plasmids containing expression cassettes for Cas9 and two different gRNAs targeting mer3 or msh4 were constructed and separately introduced into PC9×#64. Eight of the 38 dikaryotic transformants analyzed produced no basidiospores. Genomic PCR suggested that msh4 or mer3 mutations were introduced into both nuclei of seven out of eight strains. Thus, in this study, we demonstrated simultaneous gene targeting using our CRISPR/Cas9 system, which may be useful for the molecular breeding of cultivated agaricomycetes.
Introduction: Comedy and its Afterlives
Authors: Cameron John H , Stanivukovic Goran 
Published: August 2022

Abstract
This introduction is concerned with the intellectual and cultural construction of comedy from the classical period to the present, with particular emphasis on comic afterlives. By afterlives, we mean the successive re-creation of its many forms, incarnations, inspirations and adaptations, pasts and futures. The introduction lays out the theory and critical history of comedy and its afterlives; it critically engages with the variety of comic forms, and it explains the rationale for the selection of essays in, and aims of, this Special Issue. Among the topics introduced here and covered in the issue are: the fortunes of the Spanish Golden Age theatrical comedy in English translation in the seventeenth century; the influence that classical and early modern theories of comedy had on the comedies of Ben Jonson and in turn his impact on Restoration comedy; the theatrical conditions of the Comédie-Française and Molière; the comedy of the German Enlightenment; the comedy of the Irish Revival; the meta-theatrical comedy of Luigi Pirandello; and queer elements in contemporary Arab comedy.
Deletion of Mechanosensory β1-integrin From Bladder Smooth Muscle Results in Voiding Dysfunction and Tissue Remodeling
Authors: Yu Weiqun , MacIver Bryce , Zhang Lanlan , Bien Erica M , Ahmed Nazaakat , Chen Huan , Hanif Sarah Z , de Oliveira Mariana G , Zeidel Mark L , Hill Warren G 
Published: August 2022

Abstract
The bladder undergoes large shape changes as it fills and empties and experiences complex mechanical forces. These forces become abnormal in diseases of the lower urinary tract such as overactive bladder, neurogenic bladder, and urinary retention. As the primary mechanosensors linking the actin cytoskeleton to the extracellular matrix (ECM), integrins are likely to play vital roles in maintaining bladder smooth muscle (BSM) homeostasis. In a tamoxifen-inducible smooth muscle conditional knockout of β1-integrin, there was concomitant loss of α1- and α3-integrins from BSM and upregulation of αV- and β3-integrins. Masson's staining showed a reduction in smooth muscle with an increase in collagenous ECM. Functionally, mice exhibited a changing pattern of urination by voiding spot assay up to 8 wk after tamoxifen. By 8 wk, there was increased frequency with reductions in voided volume, consistent with overactivity. Cystometrograms confirmed that there was a significant reduction in intercontractile interval with reduced maximal bladder pressure. Muscle strip myography revealed a loss of contraction force in response to electrical field stimulation, that was entirely due to the loss of muscarinic contractility. Quantitative western blotting showed a loss of M3 receptor and no change in P2X1. qPCR on ECM and interstitial genes revealed loss of Ntpd2, a marker of an interstitial cell subpopulation; and an upregulation of S100A4, which is often associated with fibroblasts. Collectively, the data show that the loss of appropriate mechanosensation through integrins results in cellular and extracellular remodeling, and concomitant bladder dysfunction that resembles lower urinary tract symptoms seen in older people.
Dcifer: an IBD-based method to calculate genetic distance between polyclonal infections
Authors: Gerlovina Inna , Gerlovin Boris , Rodríguez-Barraquer Isabel , Greenhouse Bryan 
Published: August 2022

AbstractAn essential step toward reconstructing pathogen transmission and answering epidemiologically relevant questions from genomic data is obtaining pairwise genetic distance between infections. For recombining organisms such as malaria parasites, relatedness measures quantifying recent shared ancestry would provide a meaningful distance, suggesting methods based on identity by descent (IBD). While the concept of relatedness and consequently an IBD approach is fairly straightforward for individual parasites, the distance between polyclonal infections, which are prevalent in malaria, presents specific challenges, and awaits a general solution that could be applied to infections of any clonality and accommodate multiallelic (e.g. microsatellite or microhaplotype) and biallelic [single nucleotide polymorphism (SNP)] data. Filling this methodological gap, we present Dcifer (Distance for complex infections: fast estimation of relatedness), a method for calculating genetic distance between polyclonal infections, which is designed for unphased data, explicitly accounts for population allele frequencies and complexity of infection, and provides reliable inference. Dcifer’s IBD-based framework allows us to define model parameters that represent interhost relatedness and to propose corresponding estimators with attractive statistical properties. By using combinatorics to account for unobserved phased haplotypes, Dcifer is able to quickly process large datasets and estimate pairwise relatedness along with measures of uncertainty. We show that Dcifer delivers accurate and interpretable results and detects related infections with statistical power that is 2–4 times greater than that of approaches based on identity by state. Applications to real data indicate that relatedness structure aligns with geographic locations. Dcifer is implemented in a comprehensive publicly available software package.
Subjective Impairments in Olfaction and Cognition Predict Dissociated Behavioral Outcomes
Authors: Cedres Nira , Aejmelaeus-Lindström Andrea , Ekström Ingrid , Nordin Steven , Li Xin , Persson Jonas , Olofsson Jonas K 
Published: August 2022

Abstract

Background
Self-rated subjective cognitive decline (SCD) and subjective olfactory impairment (SOI) are associated with objective cognitive decline and dementia. However, their relationship and co-occurrence is unknown. We aimed to (a) describe the occurrence of SOI, SCD and their overlap in the general population; (b) compare SOI and SCD in terms of longitudinal associations with corresponding objective olfactory and cognitive measures; and (c) describe how SOI and SCD may lead to distinct sensory and cognitive outcomes.

Methods
Cognitively unimpaired individuals from the third wave of the Swedish population-based Betula study (n = 784, aged 35–90 years; 51% females) were split into self-rated SOI, SCD, overlapping SCD + SOI, and controls. Between-subject and within-subject repeated-measures MANCOVA were used to compare the groups regarding odor identification, cognition, age, sex, and education. Spearman correlation was used to assess the different patterns of association between olfaction and cognition across groups.

Results
SOI was present in 21.1%, whereas SCD was present in 9.9% of participants. According to a chi-square analysis, the SCD + SOI overlap (2.7%) is on a level that could be expected if the phenomena were independent. Odor identification in SOI showed decline at the 10-year follow-up (n = 284) and was positively associated with cognition. The SOI and SCD groups showed distinct cognitive-olfactory profiles at follow-up.

Conclusions
SOI occur independently of SCD in the population, and these risk factors are associated with different cognitive and olfactory outcomes. The biological causes underlying SOI and SCD, as well as the risk for future cognitive impairment, need further investigation.
Distribution of oceanic crust in the Enderby Basin offshore East Antarctica
Authors: Altenbernd-Lang Tabea , Jokat Wilfried , Leitchenkov German L 
Published: August 2022

SUMMARY
Seismic reflection and refraction data were collected in 2007 and 2012 to reveal the crustal fabric on a single long composite profile offshore Prydz Bay, East Antarctica. A P-wave velocity model provides insights on the crustal fabric, and a gravity-constrained density model is used to describe the crustal and mantle structure. The models show that a 230-km-wide continent–ocean transition separates stretched continental from oceanic crust along our profile. While the oceanic crust close to the continent–ocean boundary is just 3.5–5 km thick, its thickness increases northwards towards the Southern Kerguelen Plateau to 12 km. This change is accompanied by thickening of a lower crustal layer with high P-wave velocities of up to 7.5 km s–1, marking intrusive rocks emplaced beneath the mid-ocean ridge under increasing influence of the Kerguelen plume. Joint interpretations of our crustal model, seismic reflection data and magnetic data sets constrain the age and extent of oceanic crust in the research area. Oceanic crust is shown to continue to around 160 km farther south than has been interpreted in previous data, with profound implications for plate kinematic models of the region. Finally, by combining our findings with a regional magnetic data compilation and regional seismic reflection data we propose a larger extent of oceanic crust in the Enderby Basin than previously known.
Rotational remanent magnetization as a magnetic mineral diagnostic tool at low rotation rates
Authors: Hounslow Mark W , Horng Chorng-Shern , Karloukovski Vassil 
Published: August 2022

SUMMARY
Prior work on rotational remanent magnetization (RRM) and rotational anhysteretic remanent magnetization (ARMROT) has demonstrated promise for magnetic mineral identification in earth materials. One challenge has been to calibrate the measurements to magnetic mineral types and microstructural controls, since previous studies have used differing spin rates, alternating field (AF) intensities and decay times, which hinders a comparison of data sets. Using a RAPID magnetometer we show that the range of usable practical rotation rates is 0.25–3 Hz [rps] which allows a wide range of RRM and ARMROT characteristics to be utilized (at 100 mT AF field, 100 μT bias field). Sets of magnetic mineral extracts from sediments, and well characterized rock samples that contain the key magnetic minerals magnetite, pyrrhotite and greigite are used for a calibration of the RRM-ARMROT behaviour. Detrital pyrrhotite and pyrrhotite-bearing phyllites have largely small positive effective field (Bg) values (up to 6 μT), with differences in Bg and ARMROT ratios at 0.5 and 2.5 Hz [rps] allowing grain size discrimination. The positive Bg values, and changes in RRM and ARMROT with rotation rates allow distinction of pyrrhotite from magnetite and diagenetic greigite. Diagenetic greigite has Bg values of –83 to –109 μT (at 0.5 Hz [rps]) and unusual RRM variation at low rotation rates caused by anisotropy affects. In contrast to previous work, based on crushed and sized natural magnetite at high spin rates, Bg for single domain magnetite from intact bacterial magnetofossils from Upper Cretaceous Chalk has some of the lowest Bg (0–1 μT) and displays a steep decline in ARMROT with increasing rotation rates. A simple tool for particle size characterization of magnetite may be the ratio of ARMROT at spin rates 2.5 and 0.5 Hz [rps]. Stability of RRM is better studied using RRM acquisition with increasing AF field intensity, since static demagnetization imparts a nuisance gyroremanence along the field axis. Mineral microstructure, dislocations and particle interactions are likely additional effects on RRM behaviour that need more investigation.