bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ-Vps41 positively modulates Syp and its overexpression improves memory ability in aging mice
Authors: Li Yibo , Wang Hongfang , Gao Yanjing , Zhang Runjiao , Liu Qing , Xie Wenmeng , Liu Ziyu , Geng Dandan , Wang Lei 
Published: December 2022

IntroductionAge is an established risk factor for neurodegenerative disorders. Aging-related cognitive decline is a common cause of memory impairment in aging individuals, in which hippocampal synaptic plasticity and hippocampus-dependent memory formation are damaged. Circular RNAs (circRNAs) have been reported in many cognitive disorders, but their role in aging-related memory impairment is unclear.Methods: In this study, we aimed to investigate the effects of circ-Vps41 on aging-related hippocampus-dependent memory impairment and explore the potential mechanisms. Here, D-galactose was used to produce a conventional aging model resulting in memory dysfunction.ResultsCirc-Vps41 was significantly downregulated in D-galactose-induced aging in vitro and in vivo. The overexpression of circ-Vps41 could upregulate synaptophysin (Syp), thereby promoting the synaptic plasticity and alleviating cognitive impairment in aging mice. Mechanistically, we found that circ-Vps41 upregulated Syp expression by physically binding to miR-24-3p. Moreover, the miR-24-3p mimics reversed the circ-Vps41 overexpression-induced increase in Syp expression.DiscussionOverexpression of circ-Vps41 alleviated the synaptic plasticity and memory dysfunction via the miR-24-3p/Syp axis. These findings revealed circ-Vps41 regulatory network and provided new insights into its potential mechanisms for improving aging-related learning and memory impairment.
Case report: Peritumoral hepatic steatosis in a patient with a metastatic somatostatin-producing oligosymptomatic neuroendocrine neoplasm
Authors: Shao Yuming , Gui Yang , Cheng Yuejuan , Xu Jia , Chang Xiaoyan , Lv Ke 
Published: December 2022

Neuroendocrine neoplasms (NENs) comprise a heterogeneous collection of tumors derived from various neuroendocrine cells and are divided into functioning NEN and non-functioning NEN. Some NENs present with mild symptoms and can secrete somatostatin. These neoplasms are known as somatostatin-producing oligosymptomatic NENs. In this report, we describe a case of metastatic somatostatin-producing oligosymptomatic NEN with peritumoral hepatic steatosis and review the relevant literature. The patient was a 45-year-old woman who presented with mild steatorrhea and melena. A computed tomography scan revealed an enlarged pancreas protruding into the duodenum. Pathology after total pancreatectomy showed a grade 2 pancreatic NEN with positive somatostatin immunostaining. Enlarging masses on the liver were observed after the operation. Ultrasound examination revealed several lesions in the liver, with inner hypoechoic areas that showed rapid enhancement and fast washout on contrast-enhanced ultrasonography and with outer hyperechoic areas with continuous iso-enhancement. Therefore, the inner hypoechoic areas seen on contrast-enhanced ultrasonography were suspected to be true metastases. A biopsy confirmed this suspicion and indicated that the outer areas were peritumoral liver steatosis. This case highlights the importance of the imaging pattern described in this report for accurate diagnosis of metastatic NEN to avoid incorrect estimation of tumor size or a missed diagnosis on biopsy.
Approach to the child with fatigue: A focus for the general pediatrician
Authors: De Nardi Laura , Lanzetta Maria Andrea , Ghirigato Elena , Barbi Egidio , Gortani Giulia 
Published: December 2022

BackgroundFatigue is a common, nonspecific complaint commonly used to describe various conditions, ranging from a vague, subjective sense of weariness to muscular weakness, fatigability, exercise intolerance or excessive daytime somnolence. Despite its high frequency in the general population, literature addressing the approach to the child with fatigue from a general pediatrician perspective is poor. We herein propose a review of the available evidence on the topic, providing a practical framework to assist physicians in dealing with the issue.MethodsData were identified by searches of MEDLINE, UpToDate, Google Scholar and references from relevant articles. Articles published between 1990 and 2021 were considered, prioritizing systematic reviews and meta-analyses. Then, an empirically-based model of approaching the tired child was proposed according to our center experience.ResultsTo correctly characterize the meaning of fatigue reporting, specific clues from history and physical examination should be emphasized. Duration, severity, and the age at onset are to be considered. Then, specific queries about everyday activities, sleep hygiene and social domain could be useful in reaching a specific diagnosis and offering an appropriate treatment.ConclusionsWe suggest a pragmatic approach to fatigue in children based on age assessment, targeted questions, physical examination clues, and some laboratory first-level tests. This could provide pediatricians with a useful tool to discriminate the broad etiology of such a complaint, disentangling between psychological and organic causes. Further studies are needed to investigate the predictive value, specificity and sensitivity of this diagnostic workflow in managing the child with fatigue.
No ambiguity: Chemosensory-based ayurvedic classification of medicinal plants can be fingerprinted using E-tongue coupled with multivariate statistical analysis
Authors: Jayasundar Rama , Ghatak Somenath , Kumar Dushyant , Singh Aruna , Bhosle Preeti 
Published: December 2022

Background: Ayurveda, the indigenous medical system of India, has chemosensory property (rasa) as one of its major pharmacological metric. Medicinal plants have been classified in Ayurveda under six rasas/tastes—sweet, sour, saline, pungent, bitter and astringent. This study has explored for the first time, the use of Electronic tongue for studies of rasa-based classification of medicinal plants.Methods: Seventy-eight medicinal plants, belonging to five taste categories (sweet, sour, pungent, bitter, astringent) were studied along with the reference taste standards (citric acid, hydrochloric acid, caffeine, quinine, L-alanine, glycine, β-glucose, sucrose, D-galactose, cellobiose, arabinose, maltose, mannose, lactose, xylose). The studies were carried out with the potentiometry-based Electronic tongue and the data was analysed using Principle Component Analysis, Discriminant Function Analysis, Taste Discrimination Analysis and Soft Independent Modeling of Class Analogy.Results: Chemosensory similarities were observed between taste standards and the plant samples–citric acid with sour group plants, sweet category plants with sucrose, glycine, β-glucose and D-galactose. The multivariate analyses could discriminate the sweet and sour, sweet and bitter, sweet and pungent, sour and pungent plant groups. Chemosensory category of plant (classified as unknown) could also be identified.Conclusion: This preliminary study has indicated the possibility of fingerprinting the chemosensory-based ayurvedic classification of medicinal plants using E-tongue coupled with multivariate statistical analysis.
The essential role of YAP in ERα36-mediated proliferation and the epithelial-mesenchymal transition in MCF-7 breast cancer cells
Authors: Park Miso , Lee Seung Hyun , Bui Quyen Thu , Kim Young-Mi , Kang Keon Wook 
Published: December 2022

Purpose: Most breast cancers are hormone-receptor-positive, and thus the first-line therapy for them is an anti-estrogen medication such as tamoxifen. If metastasis occurs or resistance to tamoxifen develops, the 5-year survival rates for breast cancer patients significantly decrease. Hence, a better understanding of the molecular mechanisms that contribute to breast cancer aggressiveness is of great importance. ERα36 is an estrogen receptor variant that is known to be upregulated in breast cancer patients receiving tamoxifen treatment or in triple-negative breast cancer cells. However, the specific molecular mechanism underlying ERα36-induced tamoxifen-resistance is not yet fully understood.Methods: ERα36-overexpressing MCF-7 cells were constructed by either plasmid transfection using ERα36 vector or retroviral infection using ERα36-V5-His vector. Target-gene expression was assessed by Western blot analysis and real-time PCR, and YAP activation was evaluated by luciferase assays and immunofluorescence. Cell proliferation and formation of three-dimensional spheroids were evaluated using the IncuCyte S3 Live Cell Analysis System.Results: We found that the expression patterns of Hippo signaling-related genes were significantly changed in ERα36-overexpressing MCF-7 cells compared to MCF-7 cells, which were also similarly observed in tamoxifen-resistant MCF-7 cells. Specifically, the protein expression level and activity of YAP, the core downstream protein of the Hippo pathway, were significantly increased in ERα36-overexpressing MCF-7 cells compared with MCF-7 cells. The aggressive phenotypes acquired by ERα36 overexpression in MCF-7 cells were destroyed by YAP knockout. On this basis, we propose that ERα36 regulates YAP activity by a new mechanism involving Src kinase.Conclusion: Our results suggest that YAP targeting may be a new therapeutic approach to the treatment of advanced breast cancers overexpressing ERα36.
The effect of intergenerational rearing on young children’s self-control: The intervention effect of sports games
Authors: Sun Boqian , Quan Haiying , Zhang Ye 
Published: December 2022

ObjectiveTo investigate the effect of different rearing arrangements on children’s self-control and verify the promoting effect sports games might have on children’s self-control in intergenerational rearing.MethodsA total of 72 intergenerational rearing children and 66 non-intergenerational rearing children were included in Experiment 1, in which the delay-of-gratification task was used to examine the differences in self-control among children with different rearing arrangements. In experiment 2, 70 intergenerational rearing children were included as subjects, and sports games were used to improve their self-control.ResultsThe results of experiment 1 showed that compared with non-intergenerational rearing children, the self-control ability of intergenerational rearing children was poor. In experiment 2, it was found that after the intervention with sports games, the self-control ability of the children in the intergenerational rearing group was effectively improved.ConclusionAlthough intergenerational rearing arrangements have a certain negative impact on the self-control ability of children, the sports game intervention can be used to effectively develop the self-control ability of intergenerational rearing children.
Transport Properties of Critical Sulfur Hexafluoride From Multiscale Analysis of Density Fluctuations
Authors: Oprisan Ana , Morgado Dereck , Dorf David , Zoppelt Seth , Oprisan Sorinel A. , Hahn Inseob , Garrabos Yves , Lecoutre-Chabot Carole , Beysens Daniel 
Published: December 2022

Density fluctuations near critical points have a wide range of sizes limited only by the boundaries of the enclosing container. How would a fluctuating image near the critical point look if we could break it into disjoint spatial scales, like decomposing white light into narrow-band, monochromatic waves? What are the scaling laws governing each spatial scale? How are the relaxation times of fluctuations at each spatial scale related to the dynamics of fluctuations in the original image? Fluctuations near the critical point of pure fluids lead to different patterns of phase separation, which has a significant influence on the materials’ properties. Due to the diverging compressibility of pure fluids near the critical temperature, the critical phase collapses under its weight on Earth. It limits both the spatial extent of fluctuations and their duration. In microgravity, the buoyancy and convection are suppressed, and the critical state can be observed much closer to the critical point for a more extended period. Local density fluctuations induce light intensity fluctuations (the so-called “critical opalescence”), which we recorded for a sulfur hexafluoride (SF6) sample near the critical point in microgravity using the ALI (Alice Like Instrumentation insert) of the DECLIC (Dispositif pour l’Etude de la Croissance et des Liquides Critiques) facility on the International Space Station (ISS). From the very short (approximately 173 s total recording) data set very near, within 200 μK, the critical temperature, we determined the effective diffusion coefficient for fluctuations of different sizes. For transient and non-stationary data recorded very near the critical point immediately after a thermal quench that steps through critical temperature, we separated fluctuations of various sizes from the original images using the Bidimensional Empirical Mode Decomposition (BEMD) technique. Orthogonal and stationary Intrinsic Mode Function (IMF) images were analyzed using the Fourier-based Dynamic Differential Microscopy (DDM) method to extract the correlation time of fluctuations. We found that a single power-law exponent represented each IMF’s structure factor. Additionally, each Intermediate Scattering Function (ISF) was determined by fluctuations’ unique relaxation time constant. We found that the correlation time of fluctuations increases with IMF’s order, which shows that small size fluctuations have the shortest correlation time. Estimating thermophysical properties from short data sets affected by transient phenomena is possible within the BEMD framework
The Phylogenetic Relationships of the Family Sciaenidae Based on Genome-Wide Data Analysis
Authors: Han Xiaolu , Jin Shihuai , Han Zhiqiang , Gao Tianxiang 
Published: December 2022

Uncertainty and controversy exist in the phylogenetic status of the Sciaenidae family because of the limited genetic data availability. In this study, a data set of 69,098 bp, covering 309 shared orthologous genes, was extracted from 18 genomes and 5 transcriptomes of 12 species belonging to the Sciaenidae family and used for phylogenetic analysis. The maximum likelihood (ML) and Bayesian approach (BA) methods were used to reconstruct the phylogenetic trees. The resolved ML and BA trees showed similar topology, thus revealing two major evolutionary lineages within the Sciaenidae family, namely, Western Atlantic (WA) and Eastern Atlantic–Indo–West Pacific (EIP). The WA group included four species belonging to four genera: Cynoscion nebulosus, Equetus punctatus, Sciaenops ocellatus, and Micropogonias undulatus. Meanwhile, the EIP group formed one monophyletic clade, harboring eight species (Argyrosomus regius, A. japonicus, Pennahia anea, Nibea albiflora, Miichthys miiuy, Collichthys lucidus, Larimichthys polyactis, and L. crocea) from six genera. Our results indicated that the Western Atlantic (WA) group was more ancient in the studied species, while the Eastern Atlantic–Indo–West Pacific (EIP) group was a younger group. Within the studied species, the genera Collichthys and Larmichthys were the youngest lineages, and we do not suggest that Collichthys and Larmichthys should be considered as one genus. However, the origin of the Sciaenidae family and problems concerning the basal genus were not resolved because of the lack of genomes. Therefore, further sampling and sequencing efforts are needed.
Biological Control of Downy Mildew and Yield Enhancement of Cucumber Plants by Trichoderma harzianum and Bacillus subtilis (Ehrenberg) under Greenhouse Conditions
Authors: Núñez-Palenius Héctor G. , Orosco-Alcalá Blanca E. , Espitia-Vázquez Isidro , Olalde-Portugal Víctor , Hoflack-Culebro Mariana , Ramírez-Santoyo Luis F. , Ruiz-Aguilar Graciela M. L. , Cruz-Huerta Nicacio , Valiente-Banuet Juan I. 
Published: December 2022

The downy mildew disease of cucurbits is considered the most economically damaging disease of Cucurbitaceae worldwide. The causal agent, Pseudoperonospora cubensis (Berkeley & Curtis), may cause complete crop losses of cucurbits. Few commercial cucurbit cultivars are resistant to this disease. Commercially, P. cubensis is controlled primarily with synthetic fungicides that inhibit or eliminate the pathogen. Several biological agents have also been identified that provide some level of control. In our study, foliar applications of three strains of Trichoderma harzianum and two native strains of Bacillus subtilis were evaluated for the control of the disease on cucumber plants grown under commercial greenhouse conditions. The study was conducted using a completely randomized design with six individual treatments during two production cycles: fall 2015 and spring 2016. The response variables included disease incidence and severity, plant height, total yield, fruit quality, and weight. B. subtilis provided the best control over the incidence and severity of the disease in both production cycles. Interestingly, while T. harzianum was less effective at controlling the disease, it enhanced plant growth and productivity, and produced a higher number of better-quality fruits per plot. This increased yield with higher quality fruits may result in higher profit for the growers.
EKSPLORASI TRICHOKOMPOS KOTORAN KAMBING DAN JERAMI PADI TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN BAWANG MERAH (Allium ascalonicum L.)
Authors: Solo Joesviawati Arruan , Kandatong Hasanuddin , Hikmahwati Hikmahwati 
Published: December 2022

Penelitian ini dilaksanakan di Kelurahan Darma Kecamatan Polewali Kabupaten Polewali Mandar Provinsi Sulawesi Barat berlangsung pada bulan Agustus sampai bulan Oktober 2021, bertujuan untuk mempelajari tingkat keberhasilan pertumbuhan dan produksi tanaman bawang merah dengan pemberian Trichokompos kotoran kambing dan jerami padi. Metode penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan factor perlakuan penggunaan limbah organic kotoran kambing dan jerami padi yang terdiri dari TK1 : Kotoran Kambing = 80 gram / tanaman TK2 : Kotoran Kambing = 100 gram / tanaman TK3 : Limbah Jerami Padi = 80 gram / tanaman TK4 : Limbah Jerami Padi = 100 gram / tanaman TK5 : Kotoran Kambing + Limbah Jerami Padi = 80 gram / tanaman TK6 : Kotoran Kambing + Limbah Jerami Padi = 100 gram / tanaman Setiap perlakuan masing-masing diulang sebanyak 3 kali sehingga terdapat 18 unit penelitian, setiap unit terdapat 16 tanaman sehingga tanaman yang digunakan sebanyak 288 tanaman.Hasil pengamatan dan analisis data statistik menunjukkan bahwa tidak terdapat salah satu perlakuan eksplorasi Trichokompos kotoran kambing dan jerami padi yang memberikan pengaruh terbaik terhadap pertumbuhan dan produksi tanaman bawang merah (Allium ascalonicum L.). Keywords: Trichokompos Kotoran Kambing, Trichokompos Jerami Padi, bawang merah
UJI EFEKTIVITAS BERBAGAI WAKTU APLIKASI DAN DOSIS PUPUK NPK PHONSKA YANG BERAGAM TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN PADI (Oryza sativa L) PADA SISTEM PERTANAMAN HAZTON
Authors: Halim Muh Iqbal , Aliyah Mardjani , Nurdin Dahlia 
Published: December 2022

Padi (Oryza sativa L) merupakan tanaman pertanian purba berasal dari dua benua yaitu Asia dan Afrika Barat tropis dan subtropic dan merupakan bahan makanan pokok sebagian besar masyarakat Indonesia, upaya peninglatan produksi dan produktivitas tanaman padi dilakukan dengan penambahan pupuk NPK Phonska. Penelitian ini dilaksanakan di Desa Galeso, Kecamatan Wonomulyo, Kabupaten Polewali Mandar, Provinsi Sulawesi Barat pada bulan April hingga Agustus 2021, bertujuan untuk mengetahui waktu dan dosis aplikasi pupuk NPK Phonska yang terbaik untuk meningkatkan produksi dan produktivitas tanaman padi (Oryza sativa L) dengan menggunakan system tanam hazton. Penelitian ini dilaksanakan dengan menggunakan metode Rancangan Petak Terpisah (RPT) terdiri dari petak utama (waktu aplikasi pupuk NPK Phonska) terdiri dari 3 taraf yaitu : W1=1HST + 30HST, W2 =3 HST + 28 HST serta W3=5HST + 26 HST dan anak petak (dosis pupuk NPK Phonska) terdiri dari 3 taraf yaitu : D1=390g/petak, D2=546g/petak, serta D3=703g. Hasil penelitian dan analisis data statistik tentang uji efektivitas berbagai waktu aplikasi dan dosis pupuk NPK Phonska yang beragam terhadap partumbuhan dan produksi tanaman padi (Oryza sativa L) pada sistem pertanaman Hazton dapat diambil kesimpulan sebagai berikut : waktu aplikasi pupuk NPK Phonska tidak berpengaruh baik pada sluruh parameter pengamatan, Pemebrian dosis pupuk NPK Phonska tidak berpengaruh baik pada seeluruh parameter pengamatan, Interaksi waktu aplikasi dan dosis pupuk pupuk NPK Phonska tidak berpengaruh baik pada seluruh parameteer pengamatan.
UJI EFEKTIVITAS BERBAGAI DOSIS DAN INTERVAL WAKTU PEMBERIAN POC LAMUN TEHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN KACANG TANAH ( Arachis hypogea L.)
Authors: S Irfan , Aliyah Mardjani , Nurdin Dahlia 
Published: December 2022

Penelitian ini dilaksanakan di Jl. Todilaling, Kelurahan Pekkabata, Kecamatan Polewali Kabupaten Polewali Mandar, Provinsi Sulawesi Barat Dari Bulan Agustus Sampai Oktober 2021. Metode penelitian dilaksanakan dalam bentuk Rancangan Petak Terpisah (RPT), yang terdiri dari petak utama adalah pemberian POC lamun (L) yang meliputi 3 taraf yaitu: tanpa pemberian POC lamun/control (L0), 100 ml/ liter air (L1) dan 200 ml/ liter air (L3), pada anak petak yaitu interval waktu pemberian POC lamun yang terdiri dari 3 taraf yaitu: penyiraman 1 minggu sekali (W1), Penyiraman 2 minggu sekali (W2) dan penyiraman 3 minggu sekali (W3). Sehingga dalam penelitian ini terdapat 9 kombinasi perlakuan. Setiap perlakuan diulang sebanyak 3 (tiga) kali sehingga terdapat terdapat 27 unit penelitian. setiap unit penelitian terdiri dari 3 sampel tanaman sehingga jumlah keseluruhan adalah 81 tanaman Hasil penelitian dan analisis data statistik yang dilakukan dapat disimpulkan bahwa aplikasi POC lamun dan interval waktu pemberian tidak ada yang menunjukkan pengaruh yang baik terhadap pertumbuhan dan produksi tanaman kacang tanah (Arachis hypogea L.) akan tetapi, didapati pada masa generative bahwa pemberian 100 ml/liter (W1) menujukkan hasil yang lebih baik bandingkan dengan dengan perlakuan lainnya.Keywords: Kacang Tanah, POC lamun, Interval Waktu Pemberian
PENINGKATAN HASIL PEMBELAJARAN PKN MELALUI METODE DISKUSI MENGGUNAKAN MEDIA POWER POINT DI KELAS XI SMA PERINTIS SINDAGAMANIK
Authors: Dermawan Dermawan , Latief Abdul , Cryzhel Handri 
Published: December 2022

Penelitian ini dilaksanakan di SMAS Perintis Sindagamanik pada kelas XI MIPA melalui penerapan metode diskusi menggunakan media power point dengan tujuan untuk meningkatkan hasil belajar siswa kelas XI MIPA SMA Perintis Sindagamanik. subjek dalam penelitian ini adalah peserta didik kelas XI MIPA berjumlah 26 orang dan penelitian terdiri dari 2 siklus. Siklus I, 3 kali pertemuan dan siklus II juga dilaksanakan 3 kali. Pengambilan data di lakukan pada setiap akhir siklus, observasi dilakukan setiap pembelajaran yang berlangsung. Dan dianalisis dengan menggunakan analisis kuantitatif dan kualitatif. Dari pelaksanaan tindakan selama dua siklus hasil yang dicapai adalah meningkatnya aktifitas siswa dalam mengikuti pembelajaran sehingga hasil belajar yang dicapai siswa meningkat.