bluuebunny/crossref_metadata_2025_split huggingface dataset:
The cannabinoid receptor-1 gene interacts with stressful life events to increase the risk for problematic alcohol use
Authors: Bornscheuer Lisa , Lundin Andreas , Forsell Yvonne , Lavebratt Catharina , Melas Philippe A. 
Published: March 2022

AbstractProblematic alcohol use is a major contributor to the global burden of death and disabilities, and it represents a public health concern that has grown substantially following the COVID-19 pandemic. The available treatment options remain limited and to develop better pharmacotherapies for alcohol misuse we need to identify suitable biological targets. Previous research has implicated the brain’s endocannabinoid system (ECS) in psychiatric and stress-related outcomes, including substance use and habituation to repeated stress. Moreover, genetic variants in the cannabinoid-1 receptor gene (CNR1; CB1R) have been associated with personality traits, which are in turn predictors of substance use disorders. To date, however, no human genome-wide association study has provided evidence for an involvement of the ECS in substance use outcomes. One reason for this ECS-related “missing heritability” may be unexamined gene-environment interactions. To explore this possibility, we conducted cross-sectional analyses using DNA samples and stress-exposure data from a longitudinal Swedish population-based study (N = 2,915). Specifically, we genotyped rs2023239, a functional C/T single nucleotide polymorphism in CNR1, previously reported to be associated with CNR1 binding in the brain, subjective reward following alcohol intake, and alcohol cue-elicited brain activation. Our two outcomes of interest were (i) problematic alcohol use based on the Alcohol Use Disorders Identification Test (AUDIT), and (ii) personality trait scores based on the Five Factor Model. We found no baseline association between rs2023239 and problematic alcohol use or personality traits. However, there was a clear trend for interaction between rs2023239’s risk allele (C) and stressful life events (SLEs) in both childhood and adulthood, which predicted problematic alcohol use. Although not significant, there was also some indication that the risk allele interacted with child SLEs to increase scores on neuroticism. Our study supports the notion that the ECS can affect alcohol intake behaviors by interacting with life adversities and is—to the best of our knowledge—the first to focus on the interaction between CNR1 and stressors in both childhood and adulthood in humans. Further studies are warranted to confirm these findings.
Serum Folate deficiency in HCV related Hepatocellular Carcinoma
Authors: Malaguarnera Giulia , Catania Vito Emanuele , Bertino Gaetano , Drago Filippo , Madeddu Roberto , Bonfiglio Claudia , Malaguarnera Michele 
Published: March 2022

AbstractNutritional and environmental factors had been reporting in the progression of hepatocellular carcinoma (HCC). In this study, we focused our intervention in the correlation between the folate status and the progression of HCC in patients with chronic virus C (HCV) infection. Nine-eight patients, HCV positive with HCC and one hundred of patients with HCV positive liver cirrhosis (LC) and one hundred patients with HCV positive chronic hepatitis (CHC) and one hundred control subjects were enrolled. The viremia for hepatitis C patients (HCV) was determined by HCV RNA with polymerase chain reaction. HCV was confirmed by HCV RNA or a positive anti-HCV test with chronic liver disease. The comparison of folate serum levels in HCC patients vs Liver Cirrhosis (LC) patients showed a significant decrease of 1.16 ng/ml P = 0.0006 (95% CI-1.925 to − 0.395), in HCC patients versus CHC a decrease of 1.40 ng/ml P < 0.0001 (95% CI-2.16 to − 0.63), in HCC vs controls a decrease of 3.80 ng/ml P < 0.0001 (95% CI-4.56 to − 3.03). The comparison of homocysteine Hcy serum levels showed a significant increase in HCC vs LC of 4 nmol/L (P < 0.0001, 95% CI 2.77 to 5.22) versus CHC of 9 nmol/L (P < 0.0001, 95% CI 7.78 to 10.22) and vs Controls 9.30 nmol/L (P < 0.0001, 95% CI 8.07 to 10.52). With progression of HCV infection from chronic hepatitis to cirrhosis, then to HCC development, serum folate levels are progressively decreasing together with a progressive increase in serum homocysteine levels reflecting its role in disease progress and carcinogenesis.
The comparisons of expression pattern reveal molecular regulation of fruit metabolites in S. nigrum and S. lycopersicum
Authors: Heo Jung , Bang Woo Young , Jeong Jae Cheol , Park Sung-Chul , Lee Je Min , Choi Sungho , Lee Byounghee , Lee Young Koung , Kim Keunhwa , Park Soon Ju 
Published: March 2022

AbstractSolanum nigrum, known as black nightshade, is a medicinal plant that contains many beneficial metabolites in its fruit. The molecular mechanisms underlying the synthesis of these metabolites remain uninvestigated due to limited genetic information. Here, we identified 47,470 unigenes of S. nigrum from three different tissues by de novo transcriptome assembly, and 78.4% of these genes were functionally annotated. Moreover, gene ontology (GO) analysis using 18,860 differentially expressed genes (DEGs) revealed tissue-specific gene expression regulation. We compared gene expression patterns between S. nigrum and tomato (S. lycopersicum) in three tissue types. The expression patterns of carotenoid biosynthetic genes were different between the two species. Comparison of the expression patterns of flavonoid biosynthetic genes showed that 9 out of 14 enzyme-coding genes were highly upregulated in the fruit of S. nigrum. Using CRISPR-Cas9-mediated gene editing, we knocked out the R2R3-MYB transcription factor SnAN2 gene, an ortholog of S. lycopersicum ANTHOCYANIN 2. The mutants showed yellow/green fruits, suggesting that SnAN2 plays a major role in anthocyanin synthesis in S. nigrum. This study revealed the connection between gene expression regulation and corresponding phenotypic differences through comparative analysis between two closely related species and provided genetic resources for S. nigrum.
A toolkit for recombinant production of seven human EGF family growth factors in active conformation
Authors: Ferreira Arthur Schveitzer , Lopacinski Amanda , Batista Michel , Hiraiwa Priscila Mazzocchi , Guimarães Beatriz Gomes , Zanchin Nilson Ivo Tonin 
Published: March 2022

AbstractEpidermal growth factors (EGF) play a wide range of roles in embryogenesis, skin development, immune response homeostasis. They are involved in several pathologies as well, including several cancer types, psoriasis, chronic pain and chronic kidney disease. All members share the structural EGF domain, which is responsible for receptor interaction, thereby initiating transduction of signals. EGF growth factors have intense use in fundamental research and high potential for biotechnological applications. However, due to their structural organization with three disulfide bonds, recombinant production of these factors in prokaryotic systems is not straightforward. A significant fraction usually forms inclusion bodies. For the fraction remaining soluble, misfolding and incomplete disulfide bond formation may affect the amount of active factor in solution, which can compromise experimental conclusions and biotechnological applications. In this work, we describe a reliable procedure to produce seven human growth factors of the EGF family in Escherichia coli. Biophysical and stability analyses using limited proteolysis, light scattering, circular dichroism and nanoDSF show that the recombinant factors present folded and stable conformation. Cell proliferation and scratch healing assays confirmed that the recombinant factors are highly active at concentrations as low as 5 ng/ml.
Suppression of deflagration flame propagation of methane-air in tube by argon gas and explosion-eliminating chamber
Authors: Wang Quan , Xu Xiaomeng , Chang Weida , Li Zhimin , Zhang Jun , Li Rui 
Published: March 2022

AbstractTo explore the inhibitory effect of argon gas and explosion-eliminating chamber on methane-air deflagration flame propagation in the tube, based on the Φ = 120 mm, L = 5.5 m stainless steel pipeline test system to measure methane-air deflagration flame structure, flame propagation speed, and deflagration pressure. The results show that: 10–30% argon is mixed into the methane-air premixed gas with different equivalent ratios. With the increase in the mixed argon content, the tensile distortion and instability of the flame front increase, and the average value of flame propagation speed decreases by 2.52–60.0%. The first and second deflagration pressure peaks are reduced by about 13.1–62% and 17.7–86.5% respectively. The average value of the methane-air deflagration flame propagation velocity was reduced by 5.7–37.0% with the explosion-eliminating chamber laid at the nozzle. The second and third deflagration pressure peaks are reduced by about 10–30% and 50–90% respectively. The inhibitory effect of argon on the propagation of methane-air flame is considered better than the laying of the explosion-eliminating chamber under the experimental conditions.
HRTEM analysis of the aggregate structure and ultrafine microporous characteristics of Xinjiang Zhundong coal under heat treatment
Authors: Li Xueping , Zeng Qiang 
Published: March 2022

AbstractUnderstanding the change in coal structure during heat treatment is the basis of efficient and clean utilization of coal. In this study, high-resolution transmission electron microscopy (HRTEM) was used to analyse the changes in the aggregate structure and ultramicropores of Zhungdong coal samples (Xinjiang, China) that were heated from ambient temperature to 800 °C respectively. Then, the relationship between their HRTEM characteristics and the corresponding reaction activation energy were also analyzed. The results show that the length, curvature, order, layer spacing and stacking height of the aromatic layers of the coal sample vary with an increasing temperature, and are related to the activation energy of the reaction. As the temperature reaches 300 °C, the HRTEM characteristics of the heated coal samples are obviously different from those of the raw coal sample. It is shown that the length of lattice fringes is in the range of 0.3–1.15 nm which accounts for approximately 95% of the total number of fringes. The overall orientation of lattice fringes is not good, but there are two main directions. After heating, the number of naphthalenes in the coal samples decreased, while the number of larger aromatic layers increased. The distance between the aromatic layers of the coal sample decreased with an increasing stacking height, the order of the aromatic layers was enhanced, and the number of aromatic sheets with a larger curvature increased. The coal ultramicropores are mainly concentrated from 0.4 to 0.7 nm. Heat treatment reduces the total number of ultramicropores, but the maximum number of pores is increased. The non-six-membered ring and lattice defects lead to the bending of the fringes, the distribution of fatty structures affects the orientation of the fringes, and the relationship between the pore and molecular structure does not exist independently. After heat treatment, the aggregate structure and ultramicropore size of coal have a high correlation with the activation energy. The activation energy is closely related to the 0.6 nmultramicropores. However, the current experiment could not explain the underlying causes of these relationships. The aggregated state in coal is the macromolecular group formed between different aromatic structures, fat structures and other molecules, which is formed by the interaction of internal defects and pores in the molecular group. The structural differences at different temperatures therefore reflect the interaction of different macromolecules in coal.
Predictive factors of needle-knife pre-cut papillotomy failure in patients with difficult biliary cannulation
Authors: Lee Mu-Hsien , Huang Shu-Wei , Lin Cheng-Hui , Tsou Yung-Kuan , Sung Kai-Feng , Wu Chi-Huan , Liu Nai-Jen 
Published: March 2022

AbstractPredictors of needle-knife pre-cut papillotomy (NKP) failure for patients with difficult biliary cannulation has not been reported. Between 2004 and 2016, 390 patients with difficult biliary cannulation undergoing NKP were included in this single-center study. Following NKP, deep biliary cannulation failed in 95 patients (24.4%, NKP-failure group) and succeeded in 295 patients (75.6%, NKP-success group). Patient and technique factors were used to identify the predictors of initial NKP failure. Compared with the NKP-success group, periampullary diverticulum (28.4% vs. 18%, p = 0.028), surgically altered anatomy (13.7% vs. 7.1%, p = 0.049), number of cases performed by less experienced endoscopists, and bleeding during NKP (38.9% vs. 3.4%, p < 0.001), were significantly more frequent in the NKP-failure group. On multivariate analysis, surgically altered anatomy (OR 2.374, p = 0.045), endoscopists’ experience (OR 3.593, p = 0.001), and bleeding during NKP (OR 21.18, p < 0.001) were significantly associated with initial failure of NKP. In conclusion, NKP is a highly technique-sensitive procedure, as endoscopists’ experience, bleeding during NKP, and surgically altered anatomy were predictors of initial NKP failure.
Specificity of scattering of ultrashort laser pulses by molecules with polyatomic structure
Authors: Makarov D. N. , Makarova K. A. , Kharlamova A. A. 
Published: March 2022

AbstractThe theory of scattering of ultrashort laser pulses (USP) is the basis of diffraction analysis of matter using modern USP sources. At present, the peculiarities of interaction of USP with complex structures are not well developed. In general, the research focuses on the features of the interaction of USP with simple systems, these are atoms and simple molecules. Here we present a theory of scattering of ultrashort laser pulses on molecules with a multi-atomic structure, taking into account the specifics of the interaction of USP with such a substance. The simplicity of the obtained expressions allows them to be used in diffraction analysis. As an example, the scattering spectra of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are presented. It is shown that the theory developed here is more general in the scattering theory and passes into the previously known one if we consider the duration of the USP to be sufficiently long.
Network analysis shows decreased ipsilesional structural connectivity in glioma patients
Authors: Fekonja Lucius S. , Wang Ziqian , Cacciola Alberto , Roine Timo , Aydogan D. Baran , Mewes Darius , Vellmer Sebastian , Vajkoczy Peter , Picht Thomas 
Published: March 2022

AbstractGliomas that infiltrate networks and systems, such as the motor system, often lead to substantial functional impairment in multiple systems. Network-based statistics (NBS) allow to assess local network differences and graph theoretical analyses enable investigation of global and local network properties. Here, we used network measures to characterize glioma-related decreases in structural connectivity by comparing the ipsi- with the contralesional hemispheres of patients and correlated findings with neurological assessment. We found that lesion location resulted in differential impairment of both short and long connectivity patterns. Network analysis showed reduced global and local efficiency in the ipsilesional hemisphere compared to the contralesional hemispheric networks, which reflect the impairment of information transfer across different regions of a network.
OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz
Authors: Keen Alastair C. , Pedersen Maria Hauge , Lemel Laura , Scott Daniel J. , Canals Meritxell , Littler Dene R. , Beddoe Travis , Ono Yuki , Shi Lei , Inoue Asuka , Javitch Jonathan A. , Lane J. Robert 
Published: March 2022

AbstractHeterotrimeric G proteins are the main signalling effectors for G protein-coupled receptors. Understanding the distinct functions of different G proteins is key to understanding how their signalling modulates physiological responses. Pertussis toxin, a bacterial AB5 toxin, inhibits Gαi/o G proteins and has proven useful for interrogating inhibitory G protein signalling. Pertussis toxin, however, does not inhibit one member of the inhibitory G protein family, Gαz. The role of Gαz signalling has been neglected largely due to a lack of inhibitors. Recently, the identification of another Pertussis-like AB5 toxin was described. Here we show that this toxin, that we call OZITX, specifically inhibits Gαi/o and Gαz G proteins and that expression of the catalytic S1 subunit is sufficient for this inhibition. We identify mutations that render Gα subunits insensitive to the toxin that, in combination with the toxin, can be used to interrogate the signalling of each inhibitory Gα G protein.
Sentence Repetition as a Clinical Marker for Mandarin-Speaking Preschoolers With Developmental Language Disorder
Authors: Wang Danyang , Zheng Li , Lin Yuanyuan , Zhang Yiwen , Sheng Li 
Published: March 2022

Purpose:
Sentence repetition (SR) is believed to be a clinical marker for developmental language disorder (DLD) across many languages. This study explored the potential of a self-designed Mandarin SR task (MSRT) to reflect Mandarin-speaking preschoolers' language ability and to differentiate children with and without DLD in this population. Furthermore, we aimed to compare five scoring systems for evaluating children's MSRT performance.

Method:
In Study 1, the MSRT was administered to 59 typically developing (TD) children aged 3;6 (years;months) to 6;5 in China. The task was examined regarding its ability to correlate with language indices derived from children's narrative samples. In Study 2, both a TD and a DLD group were recruited to investigate the task's sensitivity, specificity, and likelihood ratios to distinguish between children with and without DLD.

Results:
Study 1 showed that, using four of the five scoring methods, TD children's performance on the MSRT significantly correlated with all the language measures derived from narratives. Study 2 showed that the MSRT was able to differentiate children with and without DLD.

Conclusion:
The MSRT is a promising tool to reflect language abilities and identify DLD in Mandarin-speaking preschoolers. Based on the current evidence, we recommend that researchers and clinicians select the number of errors in the syllable method or the binary method when scoring responses to meet their specific needs.

Supplemental Material:

https://doi.org/10.23641/asha.19386257
Phonetic Characteristics of Children's Early Words in German: Data From Typically Developing Children With Clinical Implications
Authors: Cassidy Rebekka , Aoyama Katsura , Davis Barbara L. 
Published: March 2022

Purpose:This study examined sound sequencing characteristics of German children's early words to compare with the patterns established for American English. We focused on consonant place of articulation for comparative purposes to Aoyama and Davis (2017) on children acquiring American English. Consonant–vowel–consonant (C1VC2) word forms is a frequent clinical target area for children receiving speech intervention for delays or disorders. Our goal is to provide useful background information for clinical decisions with German-speaking children.Method:Data from 13 German-speaking children were analyzed using the Child Language Data Exchange System database. First and second consonants in C1VC2word forms were coded as a labial (e.g., /p/, /b/, /m/), a coronal (alveolar and palatal, e.g., /t/, /d/, /n/), or a dorsal (velar, e.g., /k/, /g/, /ŋ/). All word types and tokens were then categorized into nine place–sequence types (e.g., labial–labial, Baum /baʊm/ “tree”; labial–coronal, mit /mɪt/ “with”; labial–velar, Weg /vɛk/ “path”).Results:C1VC2words consisting of labial–coronal and coronal–coronal sequences occurred more frequently than other sequences (e.g., labial–labial, coronal–labial). Words with labial–coronal sequences were much more frequent than the opposite coronal–labial sequences, similar to English. Some sequences involving dorsals (i.e., dorsal–labial) are relatively frequent in German words compared to English words.Conclusions:Results demonstrated both similarities and differences with previous studies of typically developing children learning American English. In both German and English, labial–coronal and coronal–coronal sequences were by far the most frequent. Sequences involving dorsal consonants were more frequent in German than in English. For clinical planning, results suggest that place-of-articulation patterns in C1VC2words are similar between German and English words (e.g., high frequencies of labial–coronal and coronal–coronal sequences). However, sequences involving dorsals are more frequent in German than in English. For intervention, this information can be used to guide selection of words containing sound sequences that are frequent for German-speaking children.
The Effect of a Graduate Course in Cued Speech on Students' Perspectives: A Pilot Study
Authors: Howerton-Fox Amanda , Veyvoda Michelle , Montgomery Jennifer L. 
Published: March 2022

Purpose:

Given the critical influence of professionals' beliefs on practice, this pilot study was designed to investigate the extent to which the Beliefs and Attitudes in Deaf Education (BADE) scale (
Clark et al., 2013
) could offer insight into the influence of a course in cued speech (CS) on preservice teachers' attitudes and beliefs.

Method:
This study used a retrospective pretest/posttest design. Before the course, participants responded to a brief demographic questionnaire and then completed the BADE. At the conclusion of the course, they took a retrospective BADE postsurvey.

Results:
Results indicated shifts in perspectives across the instructional approaches examined. Additionally, we found that our cross-perspectives analysis of the data brought researcher bias to the fore in enlightening ways, highlighting the value of collaborative research in our field.

Conclusions:
As a pilot study of the use of the BADE to investigate the ways in which a course in CS can influence preservice professionals' attitudes and beliefs, this investigation has revealed that a single course in the approach can influence students' perspectives not only on CS but also on a range of instructional approaches used in deaf education. As an unintended case study on the ways in which researcher bias influences empirical investigations, this investigation holds promise as a means of shining a spotlight on epistemological issues that are often discussed but rarely empirically analyzed in our field.
Making the Case for Single-Subject Experimental Design in Clinical Education and Supervision
Authors: Martin Kenyan L. , Brasseur Judith A. 
Published: March 2022

Purpose:
This tutorial describes single-subject experimental design (SSED) as it pertains to clinical education and supervision in the communication sciences and disorders professions. Similarities between everyday clinical practice, supervision, and SSED are highlighted, essential elements of SSED are described, five types of SSED are detailed, example research topics in supervision are proposed, and additional resources for further study are suggested.

Conclusions:
SSED is a feasible research method for busy professionals and provides promise for honing and developing evidence-based practices related to clinical education and supervision. Clinical educators and supervisors are encouraged to consider conducting SSED as part of their interactions with students, supervisees, and mentees in order to contribute to and strengthen understanding of effective clinical education and supervisory practices in speech-language pathology.