bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiparametric MRI‐Based Radiomic Signature for Preoperative Evaluation of Overall Survival in Intrahepatic Cholangiocarcinoma After Partial Hepatectomy
Authors: Yang Yang , Zou Xianlun , Zhou Wei , Yuan Guanjie , Hu Daoyu , Kuang Dong , Shen Yaqi , Xie Qingguo , Zhang Qingpeng , Hu Xuemei , Li Zhen 
Published: January 2022

BackgroundThe clinical outcomes of patients with intrahepatic cholangiocarcinoma (ICC) after partial hepatectomy remain suboptimal. Identifying patients with poor outcomes before surgery is urgently required.PurposeTo develop a multiparametric magnetic resonance imaging (MRI)‐based radiomic signature to evaluate overall survival (OS) preoperatively and to investigate its incremental value for disease stratification.Study TypeRetrospective.SubjectsOne hundred and sixty‐three patients with pathologically defined ICC, divided into training (N = 115) and validation sets (N = 48).SequenceThree‐dimensional T1‐weighted gradient‐echo sequence with and without contrast agent, T2‐weighted fast spin‐echo sequence, and diffusion‐weighted imaging with single‐shot echo‐planar sequence at 1.5 T or 3.0 T.AssessmentOS was defined as the time from the date of surgery to death or last contact. The radiomic signature was built based on the least absolute shrinkage and selection operator regression model. A clinicopathologic‐radiographic (CPR) model and a combined model integrating radiomic signature with CPR factors were developed with multivariable Cox regression models.Statistical TestsHarrell's concordance index (C‐index) was used to compare the discrimination of different models. Net reclassification index (NRI) and integrated discrimination improvement (IDI) were used to quantify the improvement of prognostic accuracy after adding radiomic signature.ResultsThe high‐risk patients of death defined by the radiomic signature showed significantly lower OS compared with low‐risk patients in validation set (3‐year OS 17.1% vs. 56.4%, P < 0.001). Integrating radiomic signature into tumor, node, and metastasis (TNM) staging system significantly improved the prognostic accuracy compared with TNM stage alone (validation set C‐index 0.745 vs. 0.649, P = 0.039, NRI improvement 39.9%–43.8%, IDI improvement 16.1%–19.4%). The radiomic signature showed no significant difference of C‐index with postoperative CPR model (validation set, 0.698 vs. 0.674, P = 0.752). Incorporating the radiomic signature into CPR model significantly improved prognostic accuracy (NRI improvement 32.5%–34.3%, IDI improvement 8.1%–12.9%).Data ConclusionMultiparametric MRI‐based radiomic signature is a potential biomarker for preoperative prognostic evaluation of ICC patients.Level of Evidence4Technical EfficacyStage 4
Plasmonic color generation in silver nanocrystal‐over‐mirror films by thermal embedment into a polymer spacer
Authors: Prezgot Daniel , Tatarchuk Stephen W. , Ianoul Anatoli 
Published: January 2022

AbstractThe production of colors by plasmonic nanostructures is an attractive prospect over dyes as they allow ultra‐high resolution, non‐fading colors. Typical techniques for producing plasmonic color patterns such as by electron beam or ion beam lithography are expensive, slow and not well scalable. This work demonstrates a simple, lithography‐free technique for producing plasmonic colors using a silver nanocube (AgNC) based nanoparticle‐over‐mirror (NPoM) system with thermally‐generated colors. AgNC's are deposited over a metal (Au or Ag) film with a polystyrene (PS) dielectric spacer. Upon heating the system past the glass‐transition temperature of PS, the AgNC embed into the polymer, reducing the AgNC/metal film distance. This results in a strong gap‐plasmon that can shift over 200 nm across the visible spectrum during the process. The thermal embedment of AgNC in NPoM systems is tunable across the visible range, producing wide, distinct color palettes depending on the metal film used. This technique can potentially be applied to plasmonic color‐patterning systems to produce high‐resolution microscale or nanoscale patterns over a large area.
A number‐between‐events control chart for monitoring finite horizon production processes
Authors: Johannssen Arne , Chukhrova Nataliya , Celano Giovanni , Castagliola Philippe 
Published: January 2022

AbstractIn this paper, we present a number‐between‐events (NBE) control chart for monitoring the fraction nonconforming in finite horizon production (FHP) processes and related specific performance measures. When monitoring fractions nonconforming in FHP processes, the common binomial ‐chart has two crucial limitations: the underlying distributional assumptions are violated when dealing with low‐volume production and a scarce efficiency in the case of processes characterized by a low fraction nonconforming. Thus, an efficient monitoring of FHP processes requires the selection of the correct underlying statistical model: in this case, a distribution from the hypergeometric family of discrete statistical distributions. An efficient statistical monitoring of processes with low fractions nonconforming can be achieved by means of discrete time‐between‐events (TBE) control charts, which count the number of units up to the appearance of a fixed number of nonconforming units in the sample. Here, we present a discrete TBE‐chart, denoted as NBE‐chart, based on the negative hypergeometric distribution that meets numerous requirements of efficient monitoring of FHP processes. The proposed control chart can be conveniently used for both low‐volume and mass production in processes with frequent changeovers.
Model reference adaptive control of piecewise affine systems with state tracking performance guarantees
Authors: Liu Tong , Buss Martin 
Published: January 2022

AbstractIn this paper, we investigate the model reference adaptive control approach for uncertain piecewise affine systems with state tracking performance guarantees. The proposed approach ensures the error metric, defined as the weighted Euclidean norm of the state tracking error, to be confined within a user‐defined time‐varying performance bound. We introduce an auxiliary performance bound to construct a barrier Lyapunov function. This auxiliary performance bound is reset at each switching instant, which prevents the barrier transgression caused by the jumps of the error metric at switching instants. The dwell time constraints are derived such that the auxiliary performance bound resides within the user‐defined performance bound. We prove that the Lyapunov function is nonincreasing at and in between the switching instants. Therefore, it does not impose extra dwell time constraints and ensures the error metric to fulfill the performance guarantees. Furthermore, we study the robust modification of the adaptive controller for the uncertain piecewise affine systems subject to unmatched disturbances. A numerical example validates the correctness of the proposed approach.
Challenges of devising a milk recipe in a hand‐reared hippopotamus (Hippopotamus amphibius)
Authors: Henry Barbara A. , Power Michael L. , Maslanka Michael T. , Rencken Camerin A. , Nollman Jennifer A. 
Published: January 2022

AbstractIn January 2017, a Nile hippopotamus (Hippopotamus amphibious) was born approximately six weeks premature at the Cincinnati Zoo & Botanical Garden. Due to the calf's weakened condition and lack of interest from the dam, management at the zoo made the decision to hand‐rear the calf. Limited published information was available on hand‐rearing this species of hippopotamus (hippo). To devise a nutritionally appropriate recipe, milk samples were acquired from the dam on Days 1, 3, 8, and 9 postpartum, and assayed for sugar, protein, fat, mineral, and water content using standard methods validated for multiple species of mammals at the Smithsonian National Zoo's Nutrition Science Laboratory. The sugar content stayed relatively constant (mean = 4.5%; range: 4.3%–4.7%). The fat consistently increased from 0.48% to 4.24% (mean = 2%). Excluding Day 9, the protein content gradually decreased from 9.56% to 6.39% (mean = 8%). The dry matter (DM) ranged from 14.38% to 16.72% (i.e., water content of 85.62%–83.28%), with the sum of the solids (sugar, protein, fat, and ash) averaging 98.5% of measured DM. Fat content was lower than expected but within the range of other artiodactyls. Between Days 1 and 8, the trend of decreasing protein and increasing fat was consistent with a change from colostrum to mature milk. The sharp increase in fat and protein with a decrease in sugar on Day 9 may indicate the beginning of the involution of the mammary gland due to lack of nursing stimulus. Utilizing this information, the Cincinnati Zoo was able to formulate a successful milk replacement recipe that allowed the calf to be raised through weaning to maturity.
WNT/beta-catenin signalling interrupts a senescence-induction cascade in human mesenchymal stem cells that restricts their expansion
Authors: Lehmann Johannes , Narcisi Roberto , Franceschini Natasja , Chatzivasileiou Danai , Boer Cindy G. , Koevoet Wendy J. L. M. , Putavet Diana , Drabek Dubravka , van Haperen Rien , de Keizer Peter L. J. , van Osch Gerjo J. V. M. , ten Berge Derk 
Published: January 2022

AbstractSenescence, the irreversible cell cycle arrest of damaged cells, is accompanied by a deleterious pro-inflammatory senescence-associated secretory phenotype (SASP). Senescence and the SASP are major factors in aging, cancer, and degenerative diseases, and interfere with the expansion of adult cells in vitro, yet little is known about how to counteract their induction and deleterious effects. Paracrine signals are increasingly recognized as important senescence triggers and understanding their regulation and mode of action may provide novel opportunities to reduce senescence-induced inflammation and improve cell-based therapies. Here, we show that the signalling protein WNT3A counteracts the induction of paracrine senescence in cultured human adult mesenchymal stem cells (MSCs). We find that entry into senescence in a small subpopulation of MSCs triggers a secretome that causes a feed-forward signalling cascade that with increasing speed induces healthy cells into senescence. WNT signals interrupt this cascade by repressing cytokines that mediate this induction of senescence. Inhibition of those mediators by interference with NF-κB or interleukin 6 signalling reduced paracrine senescence in absence of WNT3A and promoted the expansion of MSCs. Our work reveals how WNT signals can antagonize senescence and has relevance not only for expansion of adult cells but can also provide new insights into senescence-associated inflammatory and degenerative diseases.
Accuracy control for roll and sheet processed printed electronics on flexible plastic substrates
Authors: Välimäki Marja K. , Jansson Elina , Von Morgen Valentijn J. J. , Ylikunnari Mari , Väisänen Kaisa-Leena , Ontero Pekka , Kehusmaa Minna , Korhonen Pentti , Kraft Thomas M. 
Published: January 2022

AbstractFor the first time, the necessity to thermally pre-treat ubiquitously used PET substrates for printed electronics, to improve dimensional stability during manufacturing, is clearly defined. The experimental results have proven this phenomenon for both roll-to-roll (R2R) and sheet-to-sheet (S2S) processing of printed electronics. The next generation of electronics manufacturing has pushed the boundaries for low-cost, flexible, printed, and mass produced electronic components and systems. A driving force, and enabling production method, are the R2R printing presses. However, to produce electronics with increasing complexity and high yield in volume production, one must have a highly accurate process. In this article, R2R processing accuracy of printed electronics is evaluated from the point of dimensional accuracy of the flexible polyester substrate (DuPont Teijin Films’ PET Melinex ST504 with and without indium tin oxide, Melinex ST506, and Melinex PCS), precision of printing, and accuracy of layer-to-layer registration with stages that involve tension and elevated temperatures. This study has confirmed that dimensional changes during R2R processing will occur only in the first processing stage and that if a thermal pre-treatment run for the substrate is made—at identical temperature and tension of the processing stage—there is improved stability originating from a new-level strain in the crystalline PET film structure and freezing it in at the tensions and temperatures it is exposed to (i.e. 1400 μm machine direction stretching reduced to 8 μm). Furthermore, it is explained how the dimensional accuracy can be improved and reproducibly maintained in multilayer printing of electronics devices such as organic photovoltaics (OPV). These devices provide a valuable baseline of how the layer-to-layer alignment accuracy plays a crucial role in fully printed electronics devices, which lessons can be applied in all aspects of this field including hybrid systems and system fabrication involving multiple processing methods.
High-resolution human KIR genotyping
Authors: Downing Jonathan , D’Orsogna Lloyd 
Published: January 2022

AbstractKiller immunoglobulin-like receptors (KIR) regulate the function of natural killer cells through interactions with various ligands on the surface of cells, thereby determining whether natural killer (NK) cells are to be activated or inhibited from killing the cell being interrogated. The genes encoding these proteins display extensive variation through variable gene content, copy number and allele polymorphism. The combination of KIR genes and their ligands is implicated in various clinical settings including haematopoietic stem cell and solid organ transplant and infectious disease progression. The determination of KIR genes has been used as a factor in the selection of optimal stem cell donors with haplotype variations in recipient and donor giving differential clinical outcomes. Methods to determine KIR genes have primarily involved ascertaining the presence or absence of genes in an individual. With the more recent introduction of massively parallel clonal next-generation sequencing and single molecule very long read length third-generation sequencing, high-resolution determination of KIR alleles has become feasible. Determining the extent and functional impact of allele variation has the potential to lead to further optimisation of clinical outcomes as well as a deeper understanding of the functional properties of the receptors and their interactions with ligands. This review summarizes recently published high-resolution KIR genotyping methods and considers the various advantages and disadvantages of the approaches taken. In addition the application of allele level genotyping in the setting of transplantation and infectious disease control is discussed.
Biome-specific distribution of Ni-containing carbon monoxide dehydrogenases
Authors: Inoue Masao , Omae Kimiho , Nakamoto Issei , Kamikawa Ryoma , Yoshida Takashi , Sako Yoshihiko 
Published: January 2022

AbstractNi-containing carbon monoxide dehydrogenase (Ni-CODH) plays an important role in the CO/CO2-based carbon and energy metabolism of microbiomes. Ni-CODH is classified into distinct phylogenetic clades, A–G, with possibly distinct cellular roles. However, the types of Ni-CODH clade used by organisms in different microbiomes are unknown. Here, we conducted a metagenomic survey of a protein database to determine the relationship between the phylogeny and biome distribution of Ni-CODHs. Clustering and phylogenetic analyses showed that the metagenome assembly-derived Ni-CODH sequences were distributed in ~ 60% Ni-CODH clusters and in all Ni-CODH clades. We also identified a novel Ni-CODH clade, clade H. Biome mapping on the Ni-CODH phylogenetic tree revealed that Ni-CODHs of almost all the clades were found in natural aquatic environmental and engineered samples, whereas those of specific subclades were found only in host-associated samples. These results are comparable with our finding that the diversity in the phylum-level taxonomy of host-associated Ni-CODH owners is statistically different from those of the other biomes. Our findings suggest that while Ni-CODH is a ubiquitous enzyme produced across diverse microbiomes, its distribution in each clade is biased and mainly affected by the distinct composition of microbiomes.
Psoriatic arthritis with hyperuricemia: more peripheral, destructive, and challenging to treat
Authors: Widawski L. , Fabacher T. , Spielmann L. , Gottenberg JE. , Sibilia J. , Duret PM. , Messer L. , Felten R. 
Published: January 2022

Abstract
Objective
To study the impact of hyperuricemia on clinical presentation, severity, and associated comorbidities of psoriatic arthritis (PsA).

Methods
Retrospective bicentric case–control study performed in Strasbourg and Colmar, France, from 2009 to 2019. Patients with PsA (according to ICD-10 coding) and at least one available serum urate (SU) measurement were included. Demographic, comorbidities, clinical, and radiographic data were collected. Hyperuricemia was defined as SU level ≥ 360 µmol/L.

Results
We included 242 patients: 73 (30.2%) had hyperuricemia and 15 (6.2%) met 2015 ACR/EULAR criteria for gout. On univariate analysis, as compared with normo-uricemic patients, hyperuricemic patients were more frequently male (72.6% vs 39.1%, p = 1.6 × 10−6) with higher body mass index (30.9 vs 28.7 kg/m2, p = 0.015) and more comorbidities (Charlson comorbidity index: 2.6 vs 1.8, p = 0.005). PsA started at an older age (47.5 vs 43 years, p = 0.016) was more polyarticular (56.2% vs 41.9%, p = 0.049) than axial (9.6% vs 22.8%, p = 0.019) and more destructive (52.8% vs 37.4%, p = 0.032). PsA patients with joint destruction more frequently had hyperuricemia than did others (37.6% vs 25.8%, p = 0.047). Multivariable analysis confirmed the association of hyperuricemic PsA with peripheral joint involvement (odds ratio 2.98; 95% confidence interval 1.15–7.75; p = 0.025) and less good response to treatment (0.35; 0.15–0.87; p = 0.024).

Conclusion
Patients with hyperuricemic PsA show poorer response to PsA treatment and have more peripheral and destructive joint damage than normo-uricemic patients.

Key Points• Gout and psoriatic arthritis (PsA) can co-exist in the same patient.• Monosodium urate crystals might have a deleterious impact on PsA.• Hyperuricemic PsA is more polyarticular, less frequently axial, and more destructive than normo-uricemic PsA.• PsA with hyperuricemia should lead to more personalized medicine.
Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats
Authors: Liu Ying , Liu Yunen , Wu Mi , Zou Rufei , Mao Shun , Cong Peifang , Hou Mingxiao , Jin Hongxu , Zhao Yan , Bao Yongli 
Published: January 2022

AbstractBecause of stem cells are limited by the low efficiency of their cell homing and survival in vivo, cell delivery systems and scaffolds have attracted a great deal of attention for stem cells’ successful clinical practice. β-chitin nanofibers (β-ChNF) were prepared from squid pens in this study. Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy proved that β-ChNFs with the diameter of 5 to 10 nm were prepared. β-ChNF dispersion became gelled upon the addition of cell culture medium. Cell culture experiments showed that β-ChNFs exhibited negligible cytotoxicity towards ADSCs and L929 cells, and it was found that more exosomes were secreted by the globular ADSCs grown in the β-ChNF hydrogel. The vivo experiments of rats showed that the ADSCs-loaded β-ChNF hydrogel could directly cover the wound surface and significantly accelerate the wound healing and promote the generation of epithelization, granulation tissue and collagen. In addition, the ADSCs-loaded β-ChNF hydrogel clearly regulated the expressions of VEGFR, α-SMA, collagen I and collagen III. Finally, we showed that ADSCs-loaded β-ChNF hydrogel activated the TGFβ/smad signaling. The neutralization of TGFβ markedly reduced Smad phosphorylation and the expressions of TIMP1, VEGFR and α-SMA. Taken together, these findings suggest that ADSCs-loaded β-ChNF hydrogel promises for treating wounds that are challenge to heal via conventional methods.
The effect of a static magnetic field and baicalin or baicalein interactions on amelanotic melanoma cell cultures (C32)
Authors: Synowiec-Wojtarowicz Agnieszka , Pawłowska-Góral Katarzyna , Krawczyk Agata , Gawron Stanisław , Kimsa-Dudek Magdalena 
Published: January 2022

Abstract
Background
Baicalin and baicalein have antioxidant, anti-inflammatory, hepatoprotective and anti-cancer properties. However, it is not known how a static magnetic field will modify these properties. Therefore, the aim of our study was to evaluate the simultaneous exposure of melanoma cells to flavones and the static magnetic fields that are generated by permanent magnets on the gene expression and the activity of the antioxidant enzymes that are associated with the antioxidant defense system.

Methods and results
Melanoma cells that had been treated with baicalin or baicalein were subjected to a static magnetic fields with a moderate induction. The static magnetic field was emitted by permanent magnets and the cell cultures were carried out in special test chambers. The research included determining the activity of the antioxidant enzymes (superoxide dismutase, glutathione peroxidase and catalase) as well as the gene expression profile. The addition of the flavones to the cell cultures at a concentration of 50 µmol/L resulted increase in the expression of the SOD1, SOD2 and GPX1 genes compared to the nontreated cell cultures. Simultaneous exposure of the melanoma cells to static magnetic field and baicalin or baicalein reduced their mRNA levels compared to the cultures to which only baicalin or baicalein had been added. The change in gene expression was accompanied by changes at the protein level associated with an increase in the activity of antioxidant enzymes.

Conclusion
We showed that baicalin or baicalein have anticancer properties by disturbing the redox homeostasis in melanoma cells and also increases the antioxidant system gene expression. There was also an antagonistic interaction between the studied flavones and the static magnetic field, which cause a decrease in the anticancer effects of baicalin or baicalein.