bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acceptance of Clinical Decision Support System to Prevent Venous Thromboembolism Among Nurses: an Extension of the UTAUT Model
Authors: Zha Huixian , Liu Kouying , Tang Ting , Yin Yue-Heng , Dou Bei , Jiang Ling , Yan Hongyun , Wang Rong , Xie Weping 
Published: March 2022

Abstract
Background Venous thromboembolism (VTE) has been a major public health problem and caused a heavy disease burden. VTE Clinical decision support system (CDSS) was proved to have a positive influence on the prevention and management of VTE. As the direct users, nurses' acceptance of VTE CDSS is of great importance to support the successful implementation of the system. However, there are few relevant studies to investigate nurses' acceptance and the associated factors are still unclear. Objective To investigate the determinant factors of nurses' acceptance of VTE CDSS with the modified Unified Theory of Acceptance and Use of Technology (UTAUT).Methods We designed a questionnaire based on the modified UTAUT and then a cross-sectional survey was conducted among nurses in a tertiary hospital in Nanjing, China. Statistically, a Structural Equation Modeling (SEM)-Partial Least Squares (PLS) path modeling approach was applied to examine the research model.Results A total of 1100 valid questionnaires were recycled. The modified model explained 74.7%, 83.0% and 86% of the variance in user satisfaction, behavioral intention and user behavior, respectively. The results showed that performance expectancy (r=0.254, p=0.000), social influence (r=0.136, p=0.047), facilitating conditions (r=0.245, p=0.000), self-efficacy (r=0.121, p=0.048) and user satisfaction (r=0.193, p=0.001) all had significant effects on nurses' intention. Although effort expectancy (r=0.010, p=0.785) did not have a direct effect on nurses' intention, it could indirectly influence nurses' intention with user satisfaction as the mediator (r=0.296, p=0.000). User behavior was significantly predicted by facilitating conditions (r=0.298, p=0.000) and user intention (r=0.654, p=0.001). Conclusion The research enhances our understanding of the determinants of nurses' acceptance of VTE CDSS. Among these factors, performance expectancy was considered as the top priority. It highlights the importance of optimizing system performance to fit the users' needs. Generally, the findings in our research provide clinical technology designers and administrators with valuable information to better meet users' requirements and promote the implementation of VTE CDSS.
Translocator protein is a marker of activated microglia in rodent models but not human neurodegenerative diseases
Authors: Nutma Erik , Fancy Nurun , Marzin Manuel , Tsartsalis Stergios , Muirhead Robert C.J , Falk Irene , de Bruin Joy , Hollaus David , Pieterman Robin , Anink Jasper , Story David , Chandran Siddharthan , Tang Jiabin , Trolese Maria , Saito Takashi , Saido Takaomi , Weinert Maria , Moore Craig , Bendotti Caterina , Aronica Eleonora , Radulescu Carola , Jacobson Steve , Barnes Samuel , Hampton David , Valk Paul van der , Matthews Paul , Amor Sandra , Owen David 
Published: March 2022

Abstract
Microglial activation plays central roles in neuro-inflammatory and neurodegenerative diseases. Positron emission tomography (PET) targeting 18kDa Translocator Protein (TSPO) is widely used for localising inflammation in vivo, but its quantitative interpretation remains uncertain. For the first time, we show that TSPO gene and protein expression increases in activated microglia in mouse brain disease models postmortem, but does not change in a non-human primate (Callithrix jacchus) disease model or in common neurodegenerative and neuroinflammatory human diseases. We describe genetic divergence in the TSPO gene promoter that may be responsible for this, which is consistent with the hypothesis that the increase in TSPO in activated myeloid cells is unique to a subset of species within the Muroidea superfamily of rodents. These data emphasise that TSPO expression in human myeloid cells is related to different phenomena than in mice, and that TSPO PET reflects density of inflammatory cells rather than their activation state.
The proinflammatory role of guanylate binding protein 5 in inflammatory bowel diseases
Authors: Li Yichen , Lin Xutao , Gao Sheng , Wang Wenyu , Cheng Sijing , Wang Wenxia , Huang Yibo , Zou Yifeng , Zhu Ruixin , Ke Jia , Zhu Lixin 
Published: March 2022

Abstract
Background: NLRP3 inflammasome is implicated in the pathogenesis of inflammatory bowel diseases (IBD). Since guanylate binding protein 5 (GBP5) induces the NLRP3 inflammasome activity, we aim to investigate the potential role of GBP5 in IBD pathogenesis. Results: The expression of GBP5, NLRP3 inflammasome and related cytokines and chemokines were examined in two cohorts of IBD patients and healthy controls, by microarray transcriptome analysis and quantitative real time PCR. Cellular localization of GBP5 in colonic biopsies were examined by immunohistochemistry and immunofluorescence with confocal microscopy. For functional studies, GBP5 was induced by interferon γ or silenced by siRNA or CRISPR/CAS9 technique, and inflammatory activities were evaluated at mRNA and protein levels. We found that expression of GBP5 was elevated in colonic mucosa in two geographically and culturally distinct IBD cohorts. In colonic tissues of IBD patients, GBP5 expression was mainly confined to immune cells and the levels of GBP5 expression were correlated with those of the inflammatory cytokines and chemokines. In cultured T and macrophage cells, expression of proinflammatory cytokines and chemokines were increased when GBP5 was induced, while GBP5 deficiency leads to decreased expression of proinflammatory mediators including gasdermin D, caspase 1, cytokines and chemokines.Conclusions: GBP5 is required in the expression of many pro-inflammatory cytokines and chemokines in intestinal immune cells. In addition, GBP5 may up-regulate inflammatory reactions through an inflammasome mediated mechanism. Since GBP5 plays a pro-inflammatory role at the early steps of the inflammatory cascades of IBD pathogenesis, and is implicated in IBD patients of distinct genetic and environmental backgrounds, targeting GBP5 could be an effective strategy for the management of IBD.
A multi-immune gene prognostic model for evaluation of survival and prognosis of colorectal cancer
Authors: Wen Hexin , Chen Shuran , Qiu Quanwei , Wang Yitong , Zuo Lugen , Liu Mulin , Hao Bo , Dong Rui 
Published: March 2022

Abstract
Objective: We aimed to construct a multi-immune gene model for the prognosis of colorectal cancer. This study would not only provide important clinical data for the evaluation of survival and prognosis of colorectal cancer, but provide insights into the tumor immune mechanisms.Methods: Colorectal cancer gene expression and clinicopathological data were downloaded from the TCGA database, and then we performed gene expression analysis to obtain differentially expressed genes. In addition, we downloaded immune genes from the ImmPort immune gene database, and obtained differentially expressed immune genes after intersection with the differentially expressed colorectal cancer genes. We further performed survival analysis of the differential immune genes to obtain prognosis-related genes, which were used to construct a multi-immune gene prognostic model. We then analyzed the impact of the prognostic model risk score on the survival of colorectal cancer patients through survival analysis, using ROC analysis. In addition, we performed risk curve analysis to validate the accuracy of the prognostic model risk score in assessing the prognosis of colorectal cancer, and also conducted independent prognostic analysis. Finally, we analyzed the correlation between the immune genes, and transcription factors as well as immune cells.Results: Our analysis showed that prognosis of the high-risk group as evaluated by the immune gene prognosis model risk score was poor (P<0.001). The prognostic model risk score could accurately classify the colorectal patients and has high accuracy in the analysis of prognosis of colorectal cancer (AUC=0.861). Our data demonstrated a certain correlation between the immune genes, transcription factors and immune cells.Conclusions: The constructed prognostic model could accurately assess the prognosis and survival of patients with colorectal cancer. Immune genes might regulate malignant progression of tumors by modulating the production of transcription factors and immune cells. This study demonstrated the influence of immune factors on the prognosis of colorectal cancer and provided a reference for further studies evaluating the role of immunity in the development of colorectal cancer.
The Prognostic Value of Diabetes in Patients Underwent Radical Cystectomy: A Meta- analysis
Authors: LV Dong , XIANG Ying , SONG Tao , ZHOU Guang-Peng , SHEN Tai-Ming 
Published: March 2022

Abstract
Objective
To systematically evaluate the impact of diabetes on the prognosis of bladder cancer patients after radical cystectomy (RC).
Methods
PubMed, Embase, Cochrane Library database were selected from the inception to the October 2021. The studies on the effects of diabetes on bladder cancer patients after RC were included for analysis. The inclusion and exclusion criteria were independently selected for literature screening, the quality of the included studies was evaluated and data were extracted.
Results
A total of 5 cohort studies were included, with a total of 2 661 subjects, including 391 cases in the diabetic group, non-diabetes. Meta-analysis results show that diabetes increases the overall risk of death in patients after RC (HR = 1.36, 95%CI: 1.30 ~ 1.43, P < 0.001) and the risk of tumor-specific death (HR = 1.59, 95%CI: 1.29 ~ 1.95, P < 0.001). Sensitivity analysis shows that the stability of this study is well.
Conclusion
Diabetes was an independent risk factor in terms of overall and cancer-specific survival in patients underwent RC.
Activation of KYN-AHR axis impairs the chondrogenic and chondroprotective effects of human umbilical cord-derived mesenchymal stem cells on osteoarthritis
Authors: Chang Yan , Wang Xinwei , Li Susu , Wang Yueye , Jia Chengyan , Yang Xuezhi , Li Siyu , Zhang Bingjie , Zhao Yingjie , Wei Wei 
Published: March 2022

Abstract
Background: It has been proved that intra-articular injection of mesenchymal stem cells (MSCs) could alleviate cartilage damage of osteoarthritis (OA) via differentiating into chondrocytes or protecting inherent cartilage. However, how OA articular microenvironment could affect MSCs’ chondrogenic and chondroprotective efficiency has yet to be fully demonstrated. Tryptophan (Trp) metabolites, most of which are endogenous ligand for aryl hydrocarbon receptor (AHR), are abnormally increased in synovial fluid (SF) of OA patient. In this study, the effects of kynurenine (KYN), one of the most important metabolites of Trp, were evaluated on the chondrogenic and chondroprotective effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). Methods: hUC-MSCs were cultured with conditioned medium containing different proportions of OA SF, then AHR activation, proliferation and chondrogenesis of hUC-MSCs were measured. In order to determine whether the chondrogenic differentiation of hUC-MSCs were regulated by AHR, hUC-MSCs were stimulated with KYN before detecting their chondrogenesis. In vitro AHR-konck down hUC-MSCs stable cell line (shAHR-UC-MSC) was generated using lentivirus transfection. Moreover, the chondroprotective effiency of shAHR-UC-MSC was determined in OA rat model.Results: OA SF could activate AHR signaling in hUC-MSCs in a concentration-dependent manner and inhibit the chondrogenic differentiation and proliferation ability of hUC-MSCs. Similar results were observed in hUC-MSCs stimulated with KYN in vitro. AHR knockdown could remarkably eliminate the suppression effects of KYN on hUC-MSCs’ chondrogenic differentiation and proliferation ability. Notably, hUC-MSCs with AHR knockdown exhibited superior therapeutic effiency in OA rat upon intra-articular injection. Conclusion: Taken together, this study indicates that OA articular microenvironment is not conducive to the therapeutic effect of hUC-MSCs, which is related to the activation of the AHR pathway by tryptophan metabolites, thus impairs the chondrogenic and chondroprotective effects of hUC-MSCs. AHR might be a promising modification target for further improving the therapeutic efficacy of hUC-MSCs on treatment of cartilage-related diseases such as OA.
When the world stops: The impact of COVID-19 on physical activity and physical literacy
Authors: Houser Natalie , Humbert Louise , Kriellaars Dean , Erlandson Marta 
Published: March 2022

Abstract
Matched pre-during pandemic comparison (160 children) revealed a substantial reduction in physical activity (p < 0.001, rrb=0.83), environmental participation (p = 0.046, rrb=0.16), movement valuation (p < 0.001, rrb=0.61), and parent perceptions of children’s physical literacy (p < 0.001, rrb=0.56,). Examining physical activity trajectories, higher pre-pandemic physical literacy protected children from pandemic related activity decline. Emerging from the pandemic, interventions should address children’s eroded belief in movement, and consider physical literacy levels of children in individualizing movement opportunities for restoration of activity levels.
Electrodeposited Magnetic Nanoporous Membrane (MNM) with a Multi-Edge Superparamagnetic Heterogeneous Wedge Junction for High-Yield and High-Throughput Immunocapture of Specific Extracellular Vesicles and Lipoproteins
Authors: Zhang Chenguang , Huo Xiaoye , Zhu Yini , Higginbotham James , Lu Xin , Franklin Jeffrey , Vickers Kasey C. , Coffey Robert , Senapati Satyajyoti , Wang Ceming , Chang Hsueh-Chia 
Published: March 2022

Abstract
Superparamagnetic nanobeads offer several advantages over microbeads for immunocapture of specifc molecular nanocarriers (extracellular vesicles, lipoproteins, and viruses) in a bioassay: high-yield capture, reduction in incuba-tion time and higher capture capacity. However, nanobeads are difficult to “pull down” because their superparamag-netic feature requires high nanoscale magnetic field gradients in addition to high magnetic fields. Here, electroplated track-etched membrane is shown to produce a unique superparamagnetic nanowedge ring with multiple edges around each nanopore. With a uniform external magnetic field, the induced monopole and dipole of this nanowedge junction combine to produce a 10x higher nanobead trapping force. A dense nanobead suspension can be filtered through the magnetic nanoporous membrane (MNM) at a high-throughput with 99% bead capture rate. The capture yield of specific nanocarriers in heterogeneous media (filtered plasma and conditioned cell media) by nanobeads/MNM ex-ceeds 80%. Quantification of RNA cargo in captured extracellular vesicles demonstrate 60x increase in capture rate of a specific microRNA relative to magnetic bead columns. Reproducibility, low loss, and concentration-independent capture rates are also demonstrated. This new MNM material hence significantly expands the application of nano-bead immunocapture to heterogeneous physiological samples, such as blood and saliva, whose molecular analytes are cargoes of nanocarriers.
Upregulation of miR-1199-5p is associated with reduced type 2 5-α reductase in benign prostatic hyperplasia
Authors: Liu Zhanliang , Lin Zhemin , Cao Fang , Jiang Mingxin , jin Song , Cui Yun , Niu YN 
Published: March 2022

Abstract
Background
The 5-α reductase inhibitors (5-ARIs) are the first-line drug managing benign prostatic hyperplasia (BPH). Unfortunately, some patients showed no responses to 5-ARIs therapy, even suffering from worse symptoms. Although the decreased expression of 5-α reductase type 2(SRD5A2) in BPH tissues might explain the 5-ARIs therapy failure, the mechanisms underlying SRD5A2 decreased remained unelucidated.
Objectives
To investigate the mechanisms of microRNA regulating the variable expression of SRD5A2 resulting in treatment failure of 5-ARIs.
Materials and methods
The expression of SRD5A2 and microRNAs in BPH tissues and prostate cells were detected by immunohistochemistry, western blotting, and quantitative real-time PCR (qRT-PCR). Dual-luciferase reporter assay was performed to confirm that microRNA directly combine to SRD5A2 mRNA. The apoptosis of prostatic cells was detected by flow cytometry.
Results
13.6%, 28.8%, and 57.6% of BPH tissues showed negative, weak, and strong positive SRD5A2 expression, respectively. Normal human prostatic epithelial cell line RWPE-1 strongly expressed SRD5A2, whereas the immortalized human prostatic epithelial cell line BPH-1 weakly expressed SRD5A2. miR-1199-5p expression level in BPH-1 was remarkably higher than that in RWPE-1(P༜0.001), and miR-1199-5p expression was significantly upregulated in BPH tissues with negative SRD5A2 expression than those with positive SRD5A2 expression. After miR-1199-5p mimics transfection, SRD5A2 expression was decreased markedly in RWPE-1 cells, whereas after miR-1199-5p inhibitor transfection, SRD5A2 expression increased in BPH-1 cells. Dual-luciferase reporter assay showed that miR-1199-5p could bind the 3’ untranslated region of SRD5A2. Additionally, miR-1199-5p could decrease the sensibility of finasteride (100 µM) on RWPE-1 cells.
Conclusion
Our results demonstrate that SRD5A2 expression varied in BPH tissues and miR-1199-5p might be one of the several factors contributing to differential SRD5A2 expression in BPH patients.
The filamentary internal structure of the 3C279 blazar jet
Authors: Fuentes Antonio , Gomez Jose , Martí José María , Perucho Manel , Zhao Guang-Yao , Lico Rocco , Lobanov Andrei , Bruni Gabriele , Kovalev Yuri , Chael Andrew , Akiyama Kazunori , Bouman Katherine , Sun He , Cho Ilje , Traianou Efthalia , Toscano Teresa , Dahale Rohan , Gurvits Leonid , Jorstad Svetlana , Kim Jae-Young , Marscher Alan , Mizuno Yosuke , Ros Eduardo , Savolainen Tuomas 
Published: March 2022

Abstract
Supermassive black holes at the centre of active galactic nuclei power some of the most luminous objects in the Universe. Typically, very long baseline interferometric (VLBI) observations of blazars have revealed only funnel-like morphologies with little information of the ejected plasma internal structure. Here we show extremely-high angular resolution images of the blazar 3C279 obtained with the space VLBI mission RadioAstron, which allowed us to resolve the jet transversely and reveal several filaments produced by Kelvin–Helmholtz instabilities in a kinetically dominated flow that originate from the VLBI core and extend up to 175 parsecs (de-projected) downstream the jet. The VLBI core appears to be elongated, in agreement with recent Event Horizon Telescope results, but thanks to our higher dynamic range and increased field of view we are able to connect the immediate environment of the supermassive black hole with the large-scale jet. From the observed linear polarization and strong cross-section emission asymmetry we can infer that the filaments are threaded by a helical magnetic field rotating clockwise, as seen in the direction of the flow motion, with an intrinsic helix pitch angle of ~45 degrees in a jet with a Lorentz factor of ~13.
Bone-Marrow Derived Stem Cells Protocol for Premature Ovarian Failure – A case study with successful spontaneous pregnancy and delivery after treatment.
Authors: Naimat Ihsan , Alaomar Salma , Aljarrah Majed , Alkraimeen Leen , Alayan Sara , Mihai Diana , Mishra Prabhu 
Published: March 2022

Abstract
OBJECTIVES Stem cell-based therapies are emerging enormously as a ray of hope and therapeutic alternative for ovarian rejuvenation, regeneration and follicular development in women with impaired ovaries mainly due to primary ovarian insufficiency or premature ovarian failures, the conditions which cannot be improved much medically or surgically. In the modern society, the childbearing age is being delayed by women due to profound socio-economic changes making ovarian aging a key challenge for reproductive medicine. Based on the current scenario, the need of the hour is novel methods to restore fertility for all cases of ovarien impairement like poor ovarian reserve or primary ovarian insufficiency. As per the studies reported, the infusion of the whole population of human bone marrow-derived stem cells promote ovarian local vascularization, human follicle growth, increase follicle and stromal cell proliferation, and lead to reduction in apoptosis and atresia thereby, providing an adequate ovarian niche for follicular rescue in patients. MATERIAL AND METHOD Based on the previous reports, we aimed to evaluate the effects of autologous bone marrow derived stem cell transplantation into ovary on the ovarian reserve of women with impaired ovarian functions. We, therefore, designed a method to deliver bone marrow derived stem cells directly to human ovaries. RESULTS This study showed that autologous bone marrow derived stem cell ovarian transplant led to the improvement in ovarian reserve biomarkers and reproductive outcomes which results in more follicles and oocytes developed after ovarian stimulation. This technique allows successful pregnancies in women with impaired ovarian functions, however, further research is still needed as the embryo euploidy is not modified by the stem cells therapy. Diagnostic hormonal levels improved post procedure; there was a reduction in FSH and increase in AMH and estradiol levels.CONCLUSIONS Stem cells are foundation cells of the body with the remarkable abilities of self-renewal and differentiation into other cell types and this treatment bring hope to a lot of couples dealing with infertility.
Escape box and puzzle design as educational methods for engagement and satisfaction of medical student learners in emergency medicine: Survey study
Authors: Cantwell Christina , Saadat Soheil , Sakaria Sangeeta , Wiechmann Warren , Sudario Gabriel 
Published: March 2022

Abstract
Background: Gamification in medical education has gained popularity over the past several years. We describe a virtual escape box in emergency medicine clerkship didactics to teach chest pain and abdominal pain and compare this instructional method to a traditional flipped classroom format. Methods: A virtual escape box was designed at our institution and incorporated into the mandatory two-week emergency medicine clerkship. The game consisted of a PDF with four cases containing puzzles to unlock a final clue. Likert scale surveys were administered to assess participants’ perceptions of the escape box format; of clerkship didactics as a whole; and of the clerkship overall. These responses were compared to the prior year’s evaluations on flipped classroom didactics and clerkship. Results: 134 learners participated in the escape box and completed the survey. Eighty-six percent strongly agreed with feeling more engaged with the escape box, 84% strongly agreed with learning something new, 81% strongly agreed with finding the escape box to be satisfying, 78% strongly agreed with being able to apply knowledge gained, and 74% strongly agreed with wanting more escape boxes incorporated into medical education. The escape box showed a higher average score (3.6±0.63) compared to chest pain (3.5±0.67) and abdominal pain (3.2±0.77) flipped classroom sessions (p=0.0491) for the category of “lecturer explaining content clearly and at the proper level of complexity.” For the category of “lecturer provided effective instructional materials,” the escape box showed higher scores (3.6±0.63) compared to flipped classroom for chest pain (3.4±0.77) and abdominal pain (3.1±0.80) (p<0.001). Conclusions: Escape boxes are adaptable to a virtual format and can teach abstract concepts such as teamwork and communication in addition to traditional lecture content. Ratings of didactics were higher for the escape box compared to the flipped classroom, while ratings of overall clerkship experience were not found to change significantly.