bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adverse prognostic impact of the loss of STAG2 protein expression in patients with newly diagnosed localised Ewing sarcoma: A report from the Children’s Oncology Group
Authors: Shulman David S. , Chen Sonja , Hall David , Nag Anwesha , Thorner Aaron R. , Lessnick Stephen L. , Stegmaier Kimberly , Janeway Katherine A. , DuBois Steven G. , Krailo Mark D. , Barkauskas Donald A. , Church Alanna J. , Crompton Brian D. 
Published: October 2022

Abstract
Background
Ewing sarcoma (EWS) is an aggressive sarcoma with no validated molecular biomarkers. We aimed to determine the frequency of STAG2 protein loss by immunohistochemistry (IHC) and whether loss of expression is associated with outcome.

Methods
We performed a retrospective cohort study of patients with EWS enrolled to Children’s Oncology Group studies. We obtained unstained slides from 235 patients and DNA for sequencing from 75 patients. STAG2 expression was tested for association with clinical features and survival was estimated using Kaplan–Meier methods with log-rank tests.

Results
In total, 155 cases passed quality control for STAG2 IHC. STAG2 expression in 20/155 cases could not be categorised with the limited available tissue, leaving 135 patients with definitive STAG2 IHC. In localised and metastatic disease, STAG2 was lost in 29/108 and 6/27 cases, respectively. Among patients with IHC and sequencing, 0/17 STAG2 expressing cases had STAG2 mutations, and 2/7 cases with STAG2 loss had STAG2 mutations. Among patients with localised disease, 5-year event-free survival was 54% (95% CI 34–70%) and 75% (95% CI 63–84%) for patients with STAG2 loss vs. expression (P = 0.0034).

Conclusion
STAG2 loss of expression is identified in a population of patients without identifiable STAG2 mutations and carries a poor prognosis.
Caspase-1 inhibition improves cognition without significantly altering amyloid and inflammation in aged Alzheimer disease mice
Authors: Flores Joseph , Fillion Marie-Lyne , LeBlanc Andréa C. 
Published: October 2022

AbstractHuman genetic and animal model studies indicate that brain microglial inflammation is a primary driver of cognitive impairment in Alzheimer Disease (AD). Inflammasome-activated Caspase-1 (Casp1) is associated with both AD microglial inflammation and neuronal degeneration. In mice, Casp1 genetic ablation or VX-765 small molecule inhibition of Casp1 given at onset of cognitive deficits strongly supports the association between microglial inflammation and cognitive impairment. Here, VX-765 significantly improved episodic and spatial memory impairment eight months after the onset of cognitive impairment in aged AD mice with significant amyloid beta peptide (Aβ) accumulation and microglial inflammation. Unexpectedly, while cognitive improvement was associated with dendritic spine density and hippocampal synaptophysin level recovery, VX-765 only slightly decreased Aβ deposition and did not alter biochemically-measured Aβ levels. Furthermore, increased hippocampal Iba1+-microglia, GFAP+-astrocytes, IL-1β, and TNF-α levels were unaltered by VX-765. These results support the hypothesis that neuronal degeneration, not Aβ or microglial inflammation, drives cognitive impairment in AD.
Neddylation-mediated degradation of hnRNPA2B1 contributes to hypertriglyceridemia pancreatitis
Authors: Chen Wei , Wang Yilong , Xia Wenwen , Zhang Jinbao , Zhao Yan 
Published: October 2022

AbstractHypertriglyceridemia-induced acute pancreatitis (HTGP) is characterized by the acute and excessive release of FFA produced by pancreatic lipases. However, the underlying mechanisms of this disease remain poorly understood. In this study, we describe the involvement of the RNA binding protein hnRNPA2B1 in the development of HTGP. We used palmitic acid (PA) and AR42J cells to create a model of HTGP in vitro. RT-PCR and western blot analyses revealed a decrease in the level of hnRNPA2B1 protein but not mRNA expression in PA-treated cells. Further analyses revealed that hnRNPA2B1 expression was regulated at the post-translational level by neddylation. Restoration of hnRNPA2B1 expression using the neddylation inhibitor MLN4924 protected AR42J cells from PA-induced inflammatory injury by preventing NF-κB activation and restoring fatty acid oxidation and cell proliferation. Furthermore, RNA immunoprecipitation studies demonstrated that hnRNPA2B1 orchestrates fatty acid oxidation by regulating the expression of the mitochondrial trifunctional protein-α (MTPα). Administration of MLN4924 in vivo restored hnRNPA2B1 protein expression in the pancreas of hyperlipidemic mice and ameliorated HTGP-associated inflammation and pancreatic tissue injury. In conclusion, we show that hnRNPA2B1 has a central regulatory role in preventing HTGP-induced effects on cell metabolism and viability. Furthermore, our findings indicate that pharmacological inhibitors that target neddylation may provide therapeutic benefits to HTGP patients.
S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo
Authors: Real Fernando , Zhu Aiwei , Huang Boxin , Belmellat Ania , Sennepin Alexis , Vogl Thomas , Ransy Céline , Revol Marc , Arrigucci Riccardo , Lombès Anne , Roth Johannes , Gennaro Maria Laura , Bouillaud Frédéric , Cristofari Sarra , Bomsel Morgane 
Published: October 2022

AbstractHIV-1 eradication is hindered by viral persistence in cell reservoirs, established not only in circulatory CD4+T-cells but also in tissue-resident macrophages. The nature of macrophage reservoirs and mechanisms of persistence despite combined anti-retroviral therapy (cART) remain unclear. Using genital mucosa from cART-suppressed HIV-1-infected individuals, we evaluated the implication of macrophage immunometabolic pathways in HIV-1 persistence. We demonstrate that ex vivo, macrophage tissue reservoirs contain transcriptionally active HIV-1 and viral particles accumulated in virus-containing compartments, and harbor an inflammatory IL-1R+S100A8+MMP7+M4-phenotype prone to glycolysis. Reactivation of infectious virus production and release from these reservoirs in vitro are induced by the alarmin S100A8, an endogenous factor produced by M4-macrophages and implicated in “sterile” inflammation. This process metabolically depends on glycolysis. Altogether, inflammatory M4-macrophages form a major tissue reservoir of replication-competent HIV-1, which reactivate viral production upon autocrine/paracrine S100A8-mediated glycolytic stimulation. This HIV-1 persistence pathway needs to be targeted in future HIV eradication strategies.
Creating two-dimensional solid helium via diamond lattice confinement
Authors: Lin Weitong , Li Yiran , de Graaf Sytze , Wang Gang , Lin Junhao , Zhang Hui , Zhao Shijun , Chen Da , Liu Shaofei , Fan Jun , Kooi Bart J. , Lu Yang , Yang Tao , Yang Chin-Hua , Liu Chain Tsuan , Kai Ji-jung 
Published: October 2022

AbstractThe universe abounds with solid helium in polymorphic forms. Therefore, exploring the allotropes of helium remains vital to our understanding of nature. However, it is challenging to produce, observe and utilize solid helium on the earth because high-pressure techniques are required to solidify helium. Here we report the discovery of room-temperature two-dimensional solid helium through the diamond lattice confinement effect. Controllable ion implantation enables the self-assembly of monolayer helium atoms between {100} diamond lattice planes. Using state-of-the-art integrated differential phase contrast microscopy, we decipher the buckled tetragonal arrangement of solid helium monolayers with an anisotropic nature compressed by the robust diamond lattice. These distinctive helium monolayers, in turn, produce substantial compressive strains to the surrounded diamond lattice, resulting in a large-scale bandgap narrowing up to ~2.2 electron volts. This approach opens up new avenues for steerable manipulation of solid helium for achieving intrinsic strain doping with profound applications.
Sequential action of a tRNA base editor in conversion of cytidine to pseudouridine
Authors: Kimura Satoshi , Srisuknimit Veerasak , McCarty Kacie L. , Dedon Peter C. , Kranzusch Philip J. , Waldor Matthew K. 
Published: October 2022

AbstractPost-transcriptional RNA editing modulates gene expression in a condition-dependent fashion. We recently discovered C-to-Ψ editing inVibrio choleraetRNA. Here, we characterize the biogenesis, regulation, and functions of this previously undescribed RNA editing process. We show that an enzyme, TrcP, mediates the editing of C-to-U followed by the conversion of U to Ψ, consecutively. AlphaFold-2 predicts that TrcP consists of two globular domains (cytidine deaminase and pseudouridylase) and a long helical domain. The latter domain tethers tRNA substrates during both the C-to-U editing and pseudouridylation, likely enabling a substrate channeling mechanism for efficient catalysis all the way to the terminal product. C-to-Ψ editing both requires and suppresses other modifications, creating an interdependent network of modifications in the tRNA anticodon loop that facilitates coupling of tRNA modification states to iron availability. Our findings provide mechanistic insights into an RNA editing process that likely promotes environmental adaptation.
Real-world study of overall survival with palbociclib plus aromatase inhibitor in HR+/HER2− metastatic breast cancer
Authors: Rugo Hope S. , Brufsky Adam , Liu Xianchen , Li Benjamin , McRoy Lynn , Chen Connie , Layman Rachel M. , Cristofanilli Massimo , Torres Mylin A. , Curigliano Giuseppe , Finn Richard S. , DeMichele Angela 
Published: October 2022

AbstractData on real-world effectiveness of cyclin-dependent kinase 4/6 inhibitor combination therapy versus endocrine therapy alone are limited. The Flatiron Health Analytic Database was used to assess overall survival (OS) in patients with hormone receptor–positive/human epidermal growth factor receptor 2–negative (HR+/HER2−) metastatic breast cancer (MBC) treated with first-line palbociclib plus an aromatase inhibitor (AI) versus an AI alone in routine US clinical practice. In total, 2888 patients initiated treatment during February 3, 2015–March 31, 2020, with a potential ≥6-month follow-up (cutoff date, September 30, 2020). After stabilized inverse probability treatment weighting, median OS (95% CI) is significantly longer among palbociclib versus AI recipients (49.1 [45.2–57.7] versus 43.2 [37.6–48.0] months; hazard ratio, 0.76 [95% CI, 0.65–0.87]; P < 0.0001). Progression-free survival (95% CI) is 19.3 (17.5–20.7) versus 13.9 (12.5–15.2) months, respectively (hazard ratio, 0.70 [95% CI, 0.62–0.78]; P < 0.0001). These data support first-line palbociclib plus an AI treatment for HR+/HER2− MBC.(Trial number NCT05361655).
The Ecology-Culture Dataset: A new resource for investigating cultural variation
Authors: Wormley Alexandra S. , Kwon Jung Yul , Barlev Michael , Varnum Michael E. W. 
Published: October 2022

AbstractScholars interested in cultural diversity have long suggested that similarities and differences across human populations might be understood, at least in part, as stemming from differences in the social and physical ecologies individuals inhabit. Here, we describe the EcoCultural Dataset (ECD), the most comprehensive compilation to date of country-level ecological and cultural variables around the globe. ECD covers 220 countries, 9 ecological variables operationalized by 11 statistical metrics (including measures of variability and predictability), and 72 cultural variables (including values, personality traits, fundamental social motives, subjective well-being, tightness-looseness, indices of corruption, social capital, and gender inequality). This rich dataset can be used to identify novel relationships between ecological and cultural variables, to assess the overall relationship between ecology and culture, to explore the consequences of interactions between different ecological variables, and to construct new indices of cultural distance.
Crust-mantle decoupling beneath Afar revealed by Rayleigh-wave tomography
Authors: Kumar Utpal , Legendre Cédric P. 
Published: October 2022

AbstractThe Afar triple junction accustoms the diverging plate dynamics between the Arabian, Nubian, and Somalian plates along the Red Sea, Gulf of Aden, and East African rifts. The average anisotropy obtained from shear-wave splitting measurements agrees with the surface motion recovered by geodetic analyses. However, the vertical layering of anisotropy in this region is yet to be accurately determined. Here, we use earthquake seismic data to map Rayleigh-wave azimuthal anisotropy in the crust and lithospheric mantle beneath the East African Rift System. Our results suggest that a layering of anisotropy is present around the East African Rift System. At shorter periods that sample the crust, rift-parallel anisotropy is present in the vicinity of the rift, but in the central part of the rift, rift-normal anisotropy is found. At longer periods, sampling the lithospheric mantle, the anisotropic pattern is quite different. These observations suggest that the crust and lithospheric mantle are mechanically decoupled beneath the environs of the East African Rift System. Similarly, these results suggest complex dynamics within the crust and lithosphere in the region of the Afar triple junction.
The spatial stress of urban land expansion on the water environment of the Yangtze River Delta in China
Authors: Chen Yufan , Xu Yong , Zhou Kan 
Published: October 2022

AbstractIn highly urbanized and industrialized areas, the demand for construction land is expanding, which should have an impact on the water environment. Taking the Yangtze River Delta (YRD) and considering chemical oxygen demand (COD) and ammonia nitrogen (NH3-N) as characteristic pollutants, this study investigated the spatial–temporal characteristics of water pollutant emissions at the county level, optimized the spatial lag model (SLM) to estimate the spatial interaction of urban expansion and water pollutant emissions through direct and indirect effects. The results show that from 2011 to 2015, water pollutant emissions in the YRD decreased significantly and that the high-emissions pattern changed from a contiguous to a scattered distribution. The emissions of COD and NH3-N in counties at various distances from the Yangtze River and coastline show a logarithmic curve relationship. The association between urban expansion and water pollutant emissions was significant and stable. In 2015, every 1% increase in the scale of urban expansion resulted in 0.299% and 0.340% increases in local COD and NH3-N emissions, respectively, and emissions in the adjacent counties synchronously increased by 0.068% and 0.084%, respectively. The results show that to break the association and spatial interaction between urban expansion and water pollutant emissions and alleviate the environmental stress on the YRD, in addition to delimiting an urban expansion boundary and strictly restraining the scale of expansion, improvement in the regional environmental carrying capacity through urban water pollutant treatment facilities and pipe network construction is urgently needed.
Heteropolyacid coupled with cyanoguanidine decorated magnetic chitosan as an efficient catalyst for the synthesis of pyranochromene derivatives
Authors: Rahimzadeh Golnaz , Tajbakhsh Mahmood , Daraie Mansoureh , Ayati Ali 
Published: October 2022

AbstractIn this study, a novel nanocatalyst was successfully prepared by heteropolyacid immobilization of magnetic chitosan-cyanoguanidine composite and fully characterized by different analysis methods, including FTIR, XRD, TGA, SEM, and EDS. The catalytic activity of fabricated composite was examined in a one-pot three-component reaction, involving the diverse active methylene compounds, various aryl aldehydes, and malononitrile in water. The results revealed the efficient catalytic performance of composite, while all reactions proceeded smoothly and led to the formation of the corresponding pyranochromene derivatives in high to excellent yields.
Person-identifying brainprints are stably embedded in EEG mindprints
Authors: Yang Yao-Yuan , Hwang Angel Hsing-Chi , Wu Chien-Te , Huang Tsung-Ren 
Published: October 2022

AbstractElectroencephalography (EEG) signals measured under fixed conditions have been exploited as biometric identifiers. However, what contributes to the uniqueness of one's brain signals remains unclear. In the present research, we conducted a multi-task and multi-week EEG study with ten pairs of monozygotic (MZ) twins to examine the nature and components of person-identifiable brain signals. Through machine-learning analyses, we uncovered a person-identifying EEG component that served as "base signals" shared across tasks and weeks. Such task invariance and temporal stability suggest that these person-identifying EEG characteristics are more of structural brainprints than functional mindprints. Moreover, while these base signals were more similar within than between MZ twins, it was still possible to distinguish twin siblings, particularly using EEG signals coming primarily from late rather than early developed areas in the brain. Besides theoretical clarifications, the discovery of the EEG base signals has practical implications for privacy protection and the application of brain-computer interfaces.
In vitro testing of salt coating of fabrics as a potential antiviral agent in reusable face masks
Authors: Schorderet Weber Sandra , Bulliard Xavier , Bonfante Rosy , Xiang Yang , Biselli Silvia , Steiner Sandro , Constant Samuel , Pugin Raphael , Laurent Alexandra , Majeed Shoaib , Lebrun Stefan , Palmieri Michele , Hogg Andreas , Kuczaj Arkadiusz , Peitsch Manuel C. , Hoeng Julia , Stan Adrian 
Published: October 2022

AbstractDuring the coronavirus disease (COVID-19) pandemic, wearing face masks in public spaces became mandatory in most countries. The risk of self-contamination when handling face masks, which was one of the earliest concerns, can be mitigated by adding antiviral coatings to the masks. In the present study, we evaluated the antiviral effectiveness of sodium chloride deposited on a fabric suitable for the manufacturing of reusable cloth masks using techniques adapted to the home environment. We tested eight coating conditions, involving both spraying and dipping methods and three salt dilutions. Influenza A H3N2 virus particles were incubated directly on the salt-coated materials, collected, and added to human 3D airway epithelial cultures. Live virus replication in the epithelia was quantified over time in collected apical washes. Relative to the non-coated material, salt deposits at or above 4.3 mg/cm2 markedly reduced viral replication. However, even for larger quantities of salt, the effectiveness of the coating remained dependent on the crystal size and distribution, which in turn depended on the coating technique. These findings confirm the suitability of salt coating as antiviral protection on cloth masks, but also emphasize that particular attention should be paid to the coating protocol when developing consumer solutions.
Deterioration of liver function and aging disturb sequential systemic therapy for unresectable hepatocellular carcinoma
Authors: Shimose Shigeo , Hiraoka Atsushi , Tanaka Masatoshi , Iwamoto Hideki , Tanaka Takaaki , Noguchi Kazunori , Aino Hajime , Yamaguchi Taizo , Itano Satoshi , Suga Hideya , Niizeki Takashi , Moriyama Etsuko , Shirono Tomotake , Noda Yu , Kamachi Naoki , Okamura Shusuke , Nakano Masahito , Kawaguchi Takumi , Kuromatsu Ryoko , Koga Hironori , Torimura Takuji 
Published: October 2022

AbstractThis study aimed to investigate the clinical characteristics of patients with unresectable hepatocellular carcinoma (HCC), who were eligible for sequential systemic therapy. We evaluated 365 patients with HCC who underwent systemic therapy after 2017. The overall survival (OS) was 13.7 months, 19.2 months, and 35.6 months in the first-line, second-line, and third-line or later therapy groups, respectively. Multivariate analysis revealed that the modified-albumin-bilirubin (m-ALBI) grade, macrovascular invasion, extrahepatic spread, discontinuation due to adverse events (AEs), and sequential therapy were independent factors for OS. At the end of each therapy, the ALBI score was significantly worse among patients with discontinuation due to AEs than among those without. The conversion rate to second-line and third-line therapy among patients with discontinuation due to AEs was significantly lower than that among patients without (30.4% vs. 69.2%, p < 0.001; 6.7% vs. 58.3%; p < 0.001, respectively). In the decision tree analysis, m-ALBI grade 1 or 2a and non-advanced age were selected splitting variables, respectively, for sequential systemic therapy. In conclusion, sequential therapy prolonged the OS of unresectable HCC. Additionally, good hepatic function and non-advanced age were clinically eligible characteristics for sequential systemic therapy.
Association between methyl donor nutrients and metabolic health status in overweight and obese adolescents
Authors: Poursalehi Donya , Lotfi Keyhan , Mirzaei Saeideh , Asadi Ali , Akhlaghi Masoumeh , Saneei Parvane 
Published: October 2022

AbstractLimited evidence is available regarding the association of methyl donor nutrients and adolescents’ metabolic health. Therefore, we investigated the relation between a combination of methyl donor nutrients and metabolic health status of overweight and obese Iranian adolescents. In this cross-sectional study, 203 overweight/obese adolescents were included, using a multistage cluster random-sampling method. Dietary intakes were assessed by a validated food frequency questionnaire. Methyl donor nutrient score (MDNS) was constructed based on deciles of vitamins B2, B6, B9, B12, methionine, choline and betaine. Glycemic profile, lipid profile, blood pressure and anthropometric indices were collected. Participants were classified as metabolically healthy obese or unhealthy obese (MUO) based on International Diabetes Federation (IDF) and IDF/Homeostasis Model Assessment Insulin Resistance (HOMA-IR) definitions. Mean age of adolescents was 13.98 $$\pm$$
±
1.61 y and 50.2% of them was girls. After controlling all of the confounders, individuals in the top tertile of MDNS, had lower odds of MUO (OR 0.36; 95% CI 0.13–0.95) according to IFD criteria. Considering IDF/HOMA-IR criteria, an inverse marginally significant association was observed between the highest tertile of MDNS and odds of MUO (OR 0.36; 95% CI 0.12–1.02) in the fully-adjusted model. Furthermore, significant inverse association was found between each unit increase in MDNS and odds of MUO based on IDF criteria, but not for IDF/HOMA-IR definition. We found that overweight/obese adolescents with higher dietary intakes of methyl donor nutrients were less likely to be metabolically unhealthy. Further studies are needed to confirm the findings.
Facile preparation of flexible porous carbon fibers as self-supporting sulfur cathode hosts for high-performance Li–S batteries
Authors: Li Yuyun , Lei Dongyuan , Yang Shixun , Chen Jiqun , Zhao Zirui , Guo Junming , Xiang Mingwu , Liu Xiaofang , Bai Wei 
Published: October 2022

A makeup cotton derived self-supporting porous carbon fibers with a specific surface area of 2124.9 m2 g−1 are prepared. The optimized S/C cathode with a sulfur loading of 3.0 mg cm−2 delivers the first discharge capacity of 778 mA h g−1 at 0.2 C.