bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metastatic squamous cell carcinoma of colon from esophageal cancer: a case report and literature review
Authors: Mao Mian , Liu Xiqian , Liang Long , Wen Zhipeng , Liao Qiong , Xiao Hongtao , Shi LiHong 
Published: May 2022

Abstract
Background:
Esophageal cancer occupies the sixth leading cause of cancer death worldwide. Colorectal metastases are less likely to occur in squamous esophageal cancer. We report a case of metastatic squamous cell carcinoma of colon from esophageal cancer.
Case presentation:
A 66-year-old Chinese female with diabetes presented with left neck mass for 1 month. CT scan demonstrated uneven thickening wall of the mid-thoracic esophagus. A lymph node aspiration biopsy of the neck showed metastases in the lymph nodes of the left cervical region 4, which were supported by immunohistochemistry as squamous cell carcinoma. Stage ⅣA (T4N2M0) middle thoracic esophageal carcinoma was diagnosed. The patient had been treated with neoadjuvant concurrent radiotherapy and chemotherapy for 1 cycle. Then she had undergone thoracoscopic radical esophageal cancer surgery in September 2020. Pathology from esophagus and nodes showed no residual tumor and pathological stage was ypT0N0M0. In August 2021, the patient developed hematochezia, and the colonoscopy showed a nodular neoplasm with a brittle texture. Fine needle aspiration biopsy of the colon neoplasm showed squamous cell carcinoma with poorly differentiation. She subsequently received chemotherapy with oxaliplatin and irinotecan for 1 cycle. Two months later, the patient underwent a palliative resection operation and the patient died 3 months after the operation.
Conclusion:
We report the first case of metastatic squamous cell carcinoma of colon from esophageal cancer with ypT0N0M0 underwent surgery after neoadjuvant chemoradiotherapy. This case raises clinical awareness regarding colorectal metastases from esophageal cancer with pT0N0M0 underwent surgery after neoadjuvant chemoradiotherapy.
Quality appraisal for systematic literature reviews of health state utility values. A descriptive analysis
Authors: Muchadeyi Muchandifunga Trust , Hernandez-Villafuerte Karla , Schlander Michael 
Published: May 2022

Abstract
Background
Health state utility values (HSUVs) are essential input parameters to cost-utility analysis (CUA). Quality appraisal (QA) of systematic literature reviews (SLRs) of HSUVs is an important process towards the credibility of HSUVs estimates; yet, authors often overlook this crucial process. A scientifically developed and widely accepted QA tool for this purpose is lacking and warranted.
Objectives
To comprehensively describe the nature of QA in SRLs of HSUVs and generate a list of commonly used items.
Methods
A comprehensive literature search was conducted in PubMed from 01.01.2015 to 15.05.2021. SLRs of empirical studies of HSUVs that were published in English were included. Descriptive data, including QA tools checklists or good practice recommendations used or cited, items used, and methods of incorporating QA results into study findings were extracted and comprehensively reviewed. Descriptive statistics (frequencies of use and occurrences of items, acceptance and counterfactual acceptance rates) were computed. A comprehensive list of QA items was generated.
Results
A total of 68 SLRs were included, comprising 84 items and 30 QA tools and good recommendation practices. The prevalence of QA was 56% (38/68). Recommendations by NICE and ISPOR guidelines appeared in 42% (16/38) of the SLRs that appraised quality. The most commonly used QA items in SLRs were response rates (26/38), loss of follow up (21/38), statistical analysis (20/38) and sample size (20/38). Yet, the most commonly featured items in QA tools and GPRs were statistical analysis (21/30), confounding or baseline equivalency (20/30), and blinding (14/30). Only 5% of the SLRS used QA to inform the data analysis, with acceptance rates of 100% (in two studies) and 53% and 33%. A mean counterfactual acceptance rate of 55% (median 53% and IQR 56%) was observed.
Conclusions
There is a considerably low prevalence of QA in the SLRs of HSUVs. Also, there is wide a variation in the QA dimensions and items included in both SLRs and extracted tools. This underscores the need for a scientifically developed QA tool for multi-variable primary studies of HSUVs.
Effect of vitamin D deficiency on ACE2 and inflammatory factors in patients with type 2 diabetes
Authors: Xing Chenhao , Lu Yamin , Zhang Cuigai , LIU Guangxia , Chen Fang , Hou Zhan , Lv Xiuqin 
Published: May 2022

Abstract
Objective: To analyze the levels and correlation of vitamin D, ACE2, ACE and inflammatory factors in patients with type 2 diabetes,To explore the effect of vitamin D deficiency on ACE2 and inflammatory factors and the mechanism of action on the occurrence and development of diabetes.Methods: Non-diabetic control 87 cases and type 2 diabetes 96 cases,According to the level of serum 25-hydroxyvitamin D, 47 cases of non-diabetic vitamin D normal group and 40 cases of vitamin D deficiency group were divided into two groups.52 patients in the normal vitamin D group and 44 patients in the vitamin D deficient group were tested for ACE, ACE2, IL-6, TNF-a and other inflammatory indexes.The correlation and influencing factors of vitamin D and each index were analyzed.Results: The levels of HOMA-IR, ACE, AngII, IL-6 and TNF-a in vitamin D deficient group were significantly higher than those in vitamin D normal group.ACE2 and IL-10 were significantly decreased (P < 0.05); Compared with non-diabetic vitamin D deficiency group, BMI, waist circumference, blood pressure, blood lipids, blood glucose, INS, HOMA-IR, ACE, AngII, IL-6 and TNF-a were significantly increased with the severity of vitamin D deficiency. Insulin resistance was more severe, ACE2 and IL-10 decreased significantly(P < 0.05); Pearson correlation analysis showed that vitamin D in non-diabetic group was negatively correlated with AngII and IL-6, but positively correlated with IL-10 (P < 0.05 or P < 0.01).Vitamin D in diabetic group was positively correlated with ACE2 and IL-10, and negatively correlated with ACE, AngII, IL-6, TNF-a, HOMA-IR and HbA1c (all P < 0.01).Multiple linear regression analysis showed that IL-10 and AngII were the main influencing factors of vitamin D deficiency in the non-diabetic group (P < 0.05).33.6% of the total variation of regression equation was explained. In diabetic group, ACE2, IL-6, TNF-a, IL-10 and HOMA-IR were the main influencing factors of vitamin D deficiency (P < 0.05), explaining 55.8% of the total variation of regression equation.Conclusion: Vitamin D deficiency may change the improper regulation of ACE2 and ACE/AngII and the release of inflammatory factors, destroy the autoimmune state of the body, and participate in the occurrence and development of diabetes mellitus.The degree of vitamin D deficiency can aggravate insulin resistance by mediating RAS system and inflammatory factors, and increase the potential pathogenic effect of diabetes.ACE2, ACE/AngII and inflammatory factors can be used as markers of diabetes vitamin D deficiency.
ARP2/3 complex associates with peroxisomes to participate in pexophagy in plants
Authors: Martinek Jan , Cifrová Petra , Vosolsobě Stanislav , Krtková Jana , Sikorová Lenka , Malínská Kateřina , Mauerová Zdeňka , Leaves Ian , Sparkes Imogen , Schwarzerova Katerina 
Published: May 2022

Abstract
ARP2/3 is a heteroheptameric protein complex evolutionary conserved in all eukaryotic organisms. Its conserved role is based on the induction of actin polymerization at the interface between membranes and the cytoplasm. Plant ARP2/3 has been reported to participate in actin reorganization at the plasma membrane during polarized growth of trichomes and at the plasma membrane-endoplasmic reticulum contact sites. We demonstrate here that individual plant subunits of ARP2/3 fused to fluorescent proteins form motile dot-like structures in the cytoplasm that are associated with plant peroxisomes. ARP2/3 dot structure is found at the peroxisome periphery and contains assembled ARP2/3 complex and WAVE/SCAR complex subunit NAP1. This dot occasionally colocalizes with the autophagosome, and under conditions that affect the autophagy, colocalization between ARP2/3 and the autophagosome increases. ARP2/3 subunits co-immunoprecipitate with ATG8f marker. Since mutants lacking functional ARP2/3 complex have more peroxisomes than WT, we link the ARP2/3 complex on peroxisomes to the process of peroxisome degradation by autophagy called pexophagy. Additionally, several other peroxisomal proteins colocalize with ARP2/3 dot on plant peroxisomes. Our results suggest a specific role of ARP2/3 and actin in the peroxisome periphery, presumably in membrane remodelling. We hypothesize that this role of ARP2/3 aids processes at the peroxisome periphery such as peroxisome degradation through autophagy or regulation of peroxisomal proteins localization or function.
Precipitation and land use alter soil respiration in an Inner Mongolian grassland
Authors: Zhang Chi , Song Chao , Wang Donghui , Qin Wenkuan , Zhu Biao , Li Frank Yonghong , Wang Yonghui , Ma Wenhong 
Published: May 2022

Abstract
Purpose
Changes in precipitation amount and land use are expected to greatly impact soil respiration (Rs) of grasslands, but little is known about whether they can interactively impact Rs and how plant and soil microbial communities regulate the Rs response.
Methods
We investigated the impacts of altered precipitation amount (–50%, ambient and + 50%) and land use (fencing, mowing and grazing) on autotrophic (Ra), heterotrophic (Rh) and total Rs as well as their abiotic and biotic drivers with a field experiment in the Inner Mongolian grassland.
Results
Precipitation positively impacted respirations and 50% changes in precipitation leads to more substantial changes in abiotic and biotic drivers, and thus respirations, than land use. We identified the interactive effects and found that, over the 3-year experiment, a 50% decrease in precipitation caused a 31.9% decrease in Rs under fencing, but a 38.4% and a 37.2% decrease under grazing and mowing, respectively.
Conclusions
Our results indicate that taking the more intensive land use into consideration can precisely predict grassland carbon cycling in responding to future precipitation changes. In addition, chronically shifted plant and soil microbial communities under changing precipitation and land use may regulate the long-term responses of carbon cycling in grasslands.
Aptamer functionalized ZnO thin-film transistor based multiplexed detection of Lead and E. coli in water
Authors: Honnali Sanath Kumar , Ragha Vikram Srinivasa , Ashwath Roopa , Saravanavel Ganapathy , Gunasekhar K R , Sambandan Sanjiv , Gorthi Sai Siva , O’Driscoll Benjamin , Jenkins David 
Published: May 2022

Abstract
Background: Contamination of lead (Pb 2+) disturbs biological functions and causes neurotoxicity even at low levels. Pathogens such as Escherichia coli, P. aeruginosa and S. aureus found in packaged drinking water causes infections. Therefore, we demonstrate the multiplexed detection of Pb 2+ and E. coli in water using ZnO thin-film transistor (TFT) arrays. Results: Low voltage ZnO TFTs fabricated by physical vapour deposition were functionalized with APTES-glutaraldehyde molecules. TFTs with such functionalized ZnO surfaces showed good sensitivity and high specificity to the targets. The TFTs showed a detection limit of 27 nM and 10 5 cfu/ml for Pb 2+ and E. coli, respectively with the TFT flat-band voltage (V fb), and hence the TFT transconductance, being dependent on the target concentration. The direct integration of the sensing with the TFT and associated integrated circuits promises high density sensor arrays.Conclusion: The present work highlights the importance of aptamers in the multiplexed detection of different targets using low cost ZnO TFT array.
The Paris Target, Human Rights, and IPCC Weaknesses: Legal Arguments in favour of Smaller Budgets
Authors: Ekardt Felix , Bärenwaldt Marie , Heyl Katharine 
Published: May 2022

Abstract
Background:
The Paris Climate Agreement provides an overall target which limits global warming to “well below 2°C above pre-industrial levels” and pursuing “efforts to limit the temperature increase to 1.5°C above pre-industrial levels” (Art. 2 para. 1 Paris Agreement). This article assesses the extent to which new insights can be derived from recent IPCC reports for the interpretation of Art. 2 para. 1 PA from a legal perspective. To this end, the article analyses the contributions of Working Groups I and III of the sixth assessment report. We compare the findings with previously published IPCC reports, namely the 1.5 ºC report and the fifth assessment report. A legal interpretation of the Paris Agreement and of core concepts of human rights follows.
Results:
Several empirical indications show that current greenhouse gas budget calculations are quite generous. We provide six empirical arguments that clearly point in that direction. These empirical arguments demonstrate, combined with legal arguments, that the budgets have to be smaller than those estimated by the IPCC. The legal arguments are based on Art. 2 of the Paris Agreement as well as on human rights and the precautionary principle. These norms contain an obligation to minimise the risk of significant damage, i.e. to take rapid and drastic climate protection measures. 1.5 degrees Celsius is the legally binding temperature target; adherence requires a very high probability of achieving the target; temperature overshoot and geoengineering tend to be prohibited, and budget calculations must be based on sceptical factual assumptions. These findings have also been confirmed by recent rulings of supreme courts such as the ground breaking climate decision of the German Federal Constitutional Court.
Conclusions:
The Paris Agreement and human rights underline a legally binding obligation for smaller greenhouse gas budgets as those estimated in the greenhouse gas budgets of the IPCC – even compared to the 83 percent scenario in the latest assessment. Climate policy will have to raise its ambitions towards zero fossil fuels and a drastic reduction of livestock farming. This can - in times of the Ukraine war - also include policies to achieve behavioural change towards climate neutrality.
Is Canine Calprotectin in Serum Stabile After Storage at Low Temperature?
Authors: Kostanjšak Tara , Bojanić Krunoslav , Čičak Helena , Gotić Jelena , Vrbanac Zoran , Šimundić Ana-Maria , Vnuk Dražen , Bottegaro Nika Brkljača 
Published: May 2022

Abstract
Background: In human and veterinary medicine calprotectin is most widely used in diagnosing different gastro-intestinal diseases. The aim of this study was to assess the stability of canine calprotectin (cCP) in serum after storage at low temperatures and imprecision of the method. Blood samples were collected from randomly chosen dogs with different clinical diagnoses. Twenty-two dogs were included in this study. Calprotectin concentration was measured 4 hours after serum separation (T0), and after being frozen at -80 °C for 8 (T1) and 16 weeks (T2). The maximum permissible difference (MPD) was derived from the equation for calculating total error (TE) TE = %Bias + (1.96 x %CV), where bias and coefficient of variation (CV) were defined by the manufacturer.; Methods: The dogs enrolled in this study were patients admitted during the morning (9-12 a.m.), on the day the first measurement was performed. The dogs were admitted due to various reasons that were not known to the examiners at the time of sampling. All sample analysis for determination of stability were done in duplicates. For determination of within-run precision, the two patients’ serum samples were analyzed in 20 replicates. Imprecision was assessed by analyzing 20 replicates on one plate on two samples where high and low concentrations were anticipated.;Results: The calculated value of MPD was 32.52%. Median calprotectin concentrations were higher at T1 114.08 µg/L (IQR=55.05-254.56) and T2 133.6 µg/L (IQR=100.57-332.98) than at T0 83.60 µg/L (IQR=50.38-176.07). Relative and absolute bias at T1 (49.3%; 45.98 µg/L) and T2 (109.93%; 94.09 µg /L) have shown that calprotectin concentrations increase after long term storage at -80°C.; Conclusion: The results of the present study indicate that c-CP was not stable for 16 weeks at low storage temperature (-80°C). Considering the observed change in the concentration of c-CP at T1, a storage time of 8 weeks should be safely applied. The method imprecision was not satisfactory, especially in the lower concentration range.
Comparison of Tools for Postoperative Pulmonary Complication Following Cardiac surgery
Authors: Wang Yuqiang , Zhou Yaxin , Luo Zeruxin , Huang Wei , Zhang Xiu , Guo Yingqiang , Yu Pengming 
Published: May 2022

Abstract
Objectives: To review the efficacy of two scoring tools for identifying pulmonary complications after cardiac surgery.Method: A total of 244 patients underwent elective cardiac surgery between March 2021 and December 2021 were included in this observational study. Three independent physiotherapists use two different sets of PPC scoring criteria, described by Kroenke et al (Kroenke Score) and Reeve et al (Melbourne Group Scale [MGS]) to evaluate PPC status daily following surgery at midday. We then retrospective analysis the occurrence of PPC was performed 14 days postoperatively according to European Perioperative Clinical Outcome (EPCO) definitions (included atelectasis, pleural effusion, pneumonia and respiratory failure).Result: PPC frequency was 46.31% (113/244) with the Kroenke Score, 25.82% (63/244) with the Melbourne Group Scale. The clinically observed incidence of PPC (atelectasis 41.80%, pleural effusion 24.59%, pneumonia 40.57%, respiratory failure 20.08%) was 53.69% (131/244). The ROC curve shows that the overall validity of the Kroenke score is better than that of the Melbourne Group Scale (AUC, 0.94 vs. 0.88, p=0.01). The Kroenke Score performed better in open-heart surgery cohort than the Melbourne Group Scale (AUC, 0.97 vs. 0.87, p<0.01), while in interventional procedures cohort show similar (AUC, 0.90 vs. 0.86, p=0.32).Conclusion: The incidence of pulmonary complications following cardiac surgery was highly prevalent. Both the Kroenke Score and the MGS score are effective in identifying patients with postoperative pulmonary complications. The two scoring tools performed comparably in the interventional procedure population. Compared to MGS, the Kroenke Score performed better in the open-heart surgery population.Clinical Trial Registration: Chinese Clinical Trials Registry, identifier ChiCTR2100054869 (http://www.chictr.org.cn/).
Boosting For Regression Transfer via Importance Sampling
Authors: Gupta Shrey , Bi Jianzhao , Liu Yang , Wildani Avani 
Published: May 2022

Abstract
Current Instance Transfer Learning (ITL) methodologies use domain adaptation and sub-space transformation to achieve successful transfer learning. However, these methodologies, in their processes, sometimes overfit on the target dataset or suffer from negative transfer if the test dataset has a high variance. Boosting methodologies have been shown to reduce the risk of overfitting by iteratively re-weighing instances with high-residual. However, this balance is usually achieved with parameter optimization, as well as reducing the skewness in weights produced due to the size of the source dataset. While the former can be achieved, the latter is more challenging and can lead to negative transfer. We introduce a simpler and more robust fix to this problem by building upon the popular boosting ITL regression methodology, two-stage TrAdaBoost.R2. Our methodology, S-TrAdaBoost.R2, is a boosting and random-forest based ensemble methodology that utilizes importance sampling to reduce the skewness due to the source dataset. We show that S-TrAdaBoost.R2 performs better than competitive transfer learning methodologies 63% of the time. It also displays consistency in its performance over diverse datasets with varying complexities, as opposed to the sporadic results observed for other transfer learning methodologies.
Hyphomonas sediminis sp. nov., isolated from marine sediment
Authors: Wang Lu , He Wei , Gao Zeng-Yu , Zhang Dao-Feng , Huang Jian-Ke , Liu Chuang , Li Wen-Jun , Zhang Ai-Hua 
Published: May 2022

Abstract
A Gram-staining-negative, aerobic and pear-shaped bacterial strain, designated WL0036T, was isolated from coastal sediment sample collected in Nantong city, Jiangsu province of China (120°51′13″E, 32°6′26″N) in October 2020. Strain WL0036T was found to grow at 20–37°C (optimum, 28°C) with 0–9.0% NaCl (optimum, 2.5-4.0%) and displayed alkaliphilic growth with the pH range of pH 6.0–10.0 (optimum, pH 7.0–8.0). The polar lipids profile of strain WL0036T included phosphatidylcholine, phosphatidylethanolamine, glycolipid and an unidentified lipid. The major isoprenoid quinone was determined to be Q-11 and the major fatty acids were C16:0, 11-methyl-C18:1ω7c, and summed features 8 (C18:1ω6c and/or C18:1ω7c). The G + C content of genomic DNA was 61.8%. Phylogenetic trees constructed based on 16S rRNA gene sequence and bac120 gene set indicted that strain WL0036T clustered with strains Hyphomonas neptunium ATCC 15444T and H. polymorpha PS728T. The average nucleotide identities between strain WL0036T and strains H. neptunium ATCC 15444T and H. polymorpha PS728T were 80.7% and 81.2%, respectively. Strain WL0036T showed 22.8% and 23.2% of digital DNA-DNA hybridization identities with H. neptunium ATCC 15444T and H. polymorpha PS728T, respectively. As inferred from the phenotypic and genotypic characteristics and the phylogenetic trees, strain WL0036T ought to be recognized as a novel species in genus Hyphomonas, for which the name Hyphomonas sediminis sp. nov. is proposed. The type strain is WL0036T (= MCCC 1K05843T = JCM 34658T = GDMCC 1.2413T).
A Maximum Bit Rate Formulation and Design Method of FSK-based Simultaneous Wireless Power and Data Transfer Circuit
Authors: Fujita Takahiro , Wada Kazuyuki 
Published: May 2022

Abstract
A relationship between a maximum bit rate and circuit parameters of FSK-based simultaneous wireless power and data transfer system with a magnetic resonance wireless power transfer (MR-WPT) circuit is considered. The time required for switching between the two FSK carrier frequencies is formulated as a function of the circuit parameters. A design procedure for circuit parameters to achieve the desired bitrate is proposed. The validity of the example parameters determined by the proposed design procedure is confirmed by the transient simulation.