bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of guidelines for school staff on supporting students who self-harm: a Delphi study
Authors: Meinhardt Inge , Cargo Tania , Te Maro Ben , Bowden Linda , Fortune Sarah , Cuthbert Sasha , James Susanna , Cook Riley , Papalii Tania , Kapa-Kingi Korotangi , Kapa-Kingi Mariameno , Prescott Annabelle , Hetrick Sarah Elisabeth 
Published: September 2022

Abstract
Objective
Self-harm is a major public health issue that significantly impacts communities, making early intervention and prevention paramount in addressing this public health issue. This study aimed to develop evidence-based, culturally responsive, safe, and practical guidelines to assist school staff in effectively supporting students who self-harm.

Methods
This Delphi study comprised of a five-step process, oversighted by a Rōpū Mātanga Māori (Māori clinical and cultural governance group), and drawing on the expertise and knowledge gained from existing literature, interviews with stakeholders, and two panels of experts (youth and stakeholders). The Rōpū Mātanga Māori ensured accountability to the principles of Te Tiriti o Waitangi (Treaty of Waitangi) and kept Māori processes central to the research aims. The panels completed two rounds of questionnaires, rating their endorsement of each statement. Statements rated as important or essential by 80% or more of both panels and Māori participants were included in the final guidelines. The Rōpū Mātanga Māori reviewed any remaining statements to determine inclusion.

Results
Following the five-step process, 305 statements were included in the guidelines. These statements provided guiding actions that endorsed communication, collaborative responsibility, and wellbeing and a student-centred approach.

Conclusion
The guidelines provide guidance to all school staff that is culturally responsive and safe, consensus-based, and evidence-based. It is informed by the voices and experiences of young people and those who support them.
Confronting implicit bias toward patients: a scoping review of post-graduate physician curricula
Authors: Gleicher S. T. , Chalmiers M. A. , Aiyanyor B. , Jain R. , Kotha N. , Scott K. , Song R. S. , Tram J. , Vuong C. L. , Kesselheim J. 
Published: September 2022

Abstract
Background
Physicians’ behavior may unknowingly be impacted by prejudice and thereby contribute to healthcare inequities. Despite increasingly robust data demonstrating physician implicit bias (The Office of Minority Health. Minority Population Profiles, 2021; COVID-19 Shines Light on Health Disparities, National Conference of State Legislatures 2021), the evidence behind how to change this with training programs remains unclear. This scoping review therefore reports on the implementation, outcomes, and characteristics of post-graduate physician implicit bias curricula.

Methods
The authors conducted a literature review using scoping review methodology. They searched 7 databases in February and November 2020 for English-language academic and gray literature on implicit bias curricula for physicians at all levels of post-graduate training. Ten reviewers screened studies for eligibility independently, then extracted data from these studies and compiled it into a chart and analytical summary.

Results
Of the 4,599 articles screened, this review identified 90 articles on implicit bias interventions for post-graduate physicians. Inductive data analysis revealed a spectrum of educational approaches, which were categorized int o 4 educational models called Competence, Skills-Based, Social Contact, and Critical Models. The most commonly reported strength was the interactive nature of the curricula (26%), and the most frequently identified challenges were related to time and resources available (53%). Half of the interventions discussed facilitator preparation, and the majority (62%) evaluated outcomes using pre and post self-assessments.

Conclusions
This review provides a comprehensive synthesis of the literature on physician implicit bias curricula. It is our goal that this supports medical educators in applying and improving aspects of these interventions in their own programs.
Exosomes derived from heat shock preconditioned bone marrow mesenchymal stem cells alleviate cisplatin-induced ototoxicity in mice
Authors: Yang Tao , Li Wei , Peng Anquan , Wang Qin 
Published: September 2022

AbstractNOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome contributes to the development of cisplatin-induced ototoxicity. Whether heat shock pretreatment could be utilized to up-regulate 70 kilodalton heat shock proteins (HSP70) expression in bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (HS-BMSC-Exo) to alleviate cisplatin-induced ototoxicity is deciphered in this study. Heat shock pretreatment was performed on BMSCs to induce HS-BMSC-Exo, which were further trans-tympanically administrated into cisplatin intraperitoneally injected C57BL/6 mice. Auditory brainstem response (ABR) was assessed to indicate auditory sensitivity at 8, 16, 24, and 32 kHz. Myosin 7a staining was utilized to detect the mature hair cells. The relative expressions of the NLRP3 inflammasome complex were determined with Western blot in the cochlea. Diminished auditory sensitivity and increased hair cell loss could be observed in the cisplatin exposed mice with increased content of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, NLRP3, ASC, cleaved caspase-1, and pro-caspase-1, and decreased content of IL-10, which could be reversed by HS-BMSC-Exo or BMSC-Exo administration. It was worth noting that HS-BMSC-Exo demonstrated more treatment benefits than BMSC-Exo in cisplatin-induced ototoxicity. Heat shock precondition may provide a new therapeutic option to produce exosomal HSP70, and HS-BMSC-Exo could be utilized to relieve cisplatin-induced ototoxicity.
Activation of NF-κB signaling by the dense granule protein GRA15 of a newly isolated type 1 Toxoplasma gondii strain
Authors: Guo Guanghao , Cui Jianmin , Song Lindong , Tang Lvqing , Fan Sijie , Shen Bang , Fang Rui , Hu Min , Zhao Junlong , Zhou Yanqin 
Published: September 2022

Abstract
Background
It has been reported that the NF-κB pathway, an important component of host defense system against pathogens infections, can be differentially modulated by different Toxoplasma gondii strains, depending on the polymorphism of the GRA15 protein. The recently isolated Toxoplasma strain T.gHB1 is a type 1 (ToxoDB#10) strain but shows different virulence determination mechanisms compared to the classic type 1 strains like RH (ToxoDB#10). Therefore, it is worth investigating whether the T.gHB1 strain (ToxoDB#10) affects the host NF-κB signaling pathway.

Methods
The effects of T.gHB1 (ToxoDB#10) on host NF-κB pathway were investigated in HEK293T cells. The GRA15 gene product was analyzed by bioinformatics, and its effect on NF-κB activation was examined by Western blotting and nuclear translocation of p65. Different truncations of T.gHB1 GRA15 were constructed to map the critical domains for NF-κB activation.

Results
We demonstrated that the NF-κB pathway signaling pathway could be activated by the newly identified type 1 T.gHB1 strain (ToxoDB#10) of Toxoplasma, while the classic type 1 strain RH (ToxoDB#10) did not. T.gHB1 GRA15 possesses only one transmembrane region with an extended C terminal region, which is distinct from that of classic type 1 (ToxoDB#10) and type 2 (ToxoDB#1) strains. T.gHB1 GRA15 could clearly induce IκBα phosphorylation and p65 nuclear translocation. Dual luciferase assays in HEK293T cells revealed a requirement for 194–518 aa of T.gHB1 GRA15 to effectively activate NF-κB.

Conclusions
The overall results indicated that the newly isolated type 1 isolate T.gHB1 (ToxoDB#10) had a unique GRA15, which could activate the host NF-κB signaling through inducing IκBα phosphorylation and p65 nuclear translocation. These results provide new insights for our understanding of the interaction between Toxoplasma parasites and its hosts.

Graphical Abstract
Sex modifies the association between urinary albumin-to-creatinine ratio and diabetes among adults in the United States (NHANES 2011–2018)
Authors: Shi Yumeng , Hu Huan , Wu Zuxiang , Wu Ji , Chen Zhiqiang , Li Ping 
Published: September 2022

Abstract
Background
Studies on the association between urinary albumin-to-creatinine ratio (uACR) and diabetes are limited. We aimed to examine the association between uACR and diabetes among adults in the United States, with particular interest in sex differences.

Methods
Overall, 5307 participants were included in this study. The exposure variable was uACR, where uACR = urine albumin/urine creatinine. The primary outcome of this study was diabetes, defined as self-reported physician diagnosis of diabetes, fasting plasma glucose concentration ≥ 7.0 mmol/L, or use of glucose-lowering drugs.

Results
The average age of the participants in this study was 46.37 ± 17.38 years, 818 (15.41%) had diabetes and the median uACR was 7 mg/g (interquartile range, 4–12 mg/g). There was a significant positive association between uACR and diabetes (per natural log [uACR] increment: OR, 1.81; 95% CI 1.39–2.34). A multivariate logistic regression model demonstrated that per unit increment in LguACR, the diabetes prevalence increased 2.26-fold among male participants (OR 2.26, 95% CI 1.59–3.21). However, in female participants, we observed that uACR was not related to the prevalence of diabetes (odds ratio [OR], 1.28; 95% CI 0.82–2.01). Our findings showed that there was an interaction between sex and uACR (P for interaction = 0.049).

Conclusions
A higher uACR is significantly associated with an increased prevalence of diabetes, and sex can modify the relationship between them.
Resveratrol promotes liver cell survival in mice liver-induced ischemia-reperfusion through unfolded protein response: a possible approach in liver transplantation
Authors: Totonchi Hamidreza , Mokarram Pooneh , Karima Saeed , Rezaei Ramazan , Dastghaib Sanaz , Koohpeyma Farhad , Noori Shokoofe , Azarpira Negar 
Published: September 2022

Abstract
Background
Ischemia-reperfusion (I/R) of the liver is a multifactorial condition that happens during transplantation and surgery. The deleterious effects of I/R result from the acute production of reactive oxygen species (ROS), which can trigger immediate tissue damage and induce a series of destructive cellular responses, including apoptosis organ failure and inflammation. The production of ROS in the I/R process can damage the antioxidant system and cause liver damage. Resveratrol has been shown to have antioxidant properties in several investigations. Here, we address the therapeutic effect of resveratrol on I/R-induced liver injury by focusing on unfolded protein response (UPR) signaling pathway.

Methods
Five minutes before reperfusion, resveratrol was injected into the tail vein of mice. They were ischemic for 1 h and then re-perfused for 3 h before being slaughtered (I/R). The activity of liver enzymes and the expression levels of genes involved in the unfolded protein response pathway were used to measure the hepatic damage.

Results
Our results revealed that the low dose of resveratrol (0.02 and 0.2 mg/kg) post-ischemic treatment significantly reduced the ALT and AST levels. In addition, compared with the control group, the expression of UPR pathway genes GRP78, PERK, IRE1α, CHOP, and XBP1 was significantly reduced in the resveratrol group. In the mice that received lower doses of resveratrol (0.02 and 0.2 mg/kg), the histopathological changes induced by I/R were significantly improved; however, the highest dose (2 mg/kg) of resveratrol could not significantly protect and solve the I/R damage.

Conclusion
The findings of this study suggest that hepatic ischemia occurs after liver transplantation and that receiving low-dose resveratrol treatment before reperfusion may promote graft survival through inhibition of UPR arms, especially PERK and IRE1α.
Functional disability and health-related quality of life among systemic sclerosis patients in Bangladesh
Authors: Hossain Sigma , Choudhury Minhaj Rahim , Haque Md. Mahmudul , Yeasmin Surayea , Hossain Farzana , Zaman Mohammad Mostafa 
Published: September 2022

Abstract
Objective
To assess the relationship between functional disability and health-related quality of life (HRQoL) among systemic sclerosis (SSc) patients.

Methodology
This cross-sectional study was carried out on 78 adults who met the classification criteria for SSc defined by the American College of Rheumatology/European League of Rheumatology (ACR/EULAR)-2013. The Bangla version of Short Form 36 (SF-36) and Health Assessment Questionnaire-Disability Index (HAQ-DI) were used to measure HRQoL and functional disability in SSc patients.

Results
The patients' median [IQR] HAQ-DI was 1.4 [0.6–2.1], with 37.2% having a mild functional disability, 33.3 percent having a moderate functional disability, and 29.5 percent having a severe functional disability. The hygiene and activity domains of the HAQ-DI obtained the highest scores, 2.0 [0.0–3.0] and 2.0 [1.0–3.0], respectively. The Physical Component Summary (PCS) and Mental Component Summary (MCS) of the SF-36 had median [IQR] values of 26.2 [15.0–58.1] and 42.0 [19.6–60.6]. The highest score was 50.0 [25.0–75.0] in social functioning. The PCS of the SF-36 was moderately correlated with the HAQ-DI (rs = − 0.629, P < 0.001) and the MCS of the SF-36 was weakly correlated with the HAQ-DI ((rs = − 0.344, P < 0.001). Age, female sex, and incomplete fist closure substantially influenced functional status. Calcinosis, Raynaud's Phenomenon, and flexion contracture significantly diminished the quality of life.

Conclusions
Functional disability negatively affects health-related quality of life. Age, Musculoskeletal, and skin involvement are significantly associated with poor quality of life and functional disability. Therefore, treatment strategies should be aimed at reducing functional disability, which will enhance the HRQoL of SSc patients.
Endogenous Diurnal Patterns of Adrenal and Gonadal Hormones During a 24-Hour Constant Routine After Simulated Shift Work
Authors: Kelly Monica R , Yuen Fiona , Satterfield Brieann C , Auchus Richard J , Gaddameedhi Shobhan , Van Dongen Hans P A , Liu Peter Y 
Published: September 2022

Abstract

Context
Night-shift work causes circadian misalignment, predicts the development of metabolic diseases, and complicates the interpretation of hormone measurements.

Objective
To investigate endogenous circadian rhythms, dissociated from behavioral and environmental confounds, in adrenal and gonadal steroids after simulated shift work.

Methods
Fourteen healthy adults (ages 25.8 ± 3.2 years) were randomized to 3 days of night or day (control) shift work followed by a constant routine protocol designed to experimentally unveil rhythms driven endogenously by the central circadian pacemaker. Blood was sampled every 3 hours for 24 hours during the constant routine to concurrently obtain 16 Δ4 steroid profiles by mass spectrometry. Cosinor analyses of these profiles provided mesor (mean abundance), amplitude (oscillation magnitude), and acrophase (peak timing).

Results
Night-shift work marginally increased cortisol by 1 μg/dL (P = 0.039), and inactive/weak derivatives cortisone (P = 0.003) and 18-hydroxycortisol (P &lt; 0.001), but did not alter the mesor of potent androgens testosterone and 11-ketotestosterone. Adrenal-derived steroids, including 11-ketotestosterone (P &lt; 0.01), showed robust circadian rhythmicity after either day- or night-shift work. In contrast, testosterone and progesterone showed no circadian pattern after both shift work conditions. Night-shift work did not alter the amplitude or acrophase of any of the steroid profiles.

Conclusion
Experimental circadian misalignment had minimal effects on steroidogenesis. Adrenal steroids, but not gonadal hormones, showed endogenous circadian regulation robust to prior shift schedule. This dichotomy may predispose night-shift workers to metabolic ill health. Furthermore, adrenal steroids, including cortisol and the main adrenal androgen 11-ketostosterone, should always be evaluated during the biological morning whereas assessment of gonadal steroids, particularly testosterone, is dependent on the shift-work schedule.
Disease and viruses as negative factor prohibiting the growth of broiler chicken embryo as research topic trend: a bibliometric review
Authors: Mafruchati Maslichah , Kusuma Wardhana Akhmad , Wan Ismail Wan Iryani 
Published: September 2022

Background Broiler chickens have properties as meat-producing poultry and produce meat with soft fiber quality. Broiler’s embryo needs to be given attention, especially against disease. The purpose of the study was to observe the trend of the research topic and external factors which could affect the embryo’s growth of broiler. Methods This study used meta-data from Scopus. There were 162 samples started from 2006-to 2022. The data were analyzed using a bibliometric method with two software, Vosviewer, and Biblioshiy from R studio. Results It was found that the study of broiler chicken embryos had developed well, especially those related to genetics, disease, and also the immunity system. But the result showed that topics about the disease, viruses, and bacteria were more popular than topics about the gene. The result also showed that based on the importance and development of topics, the words such as “chicken”, “genes”, “development”, “effect”, growth”, and “control” had importance for the study and developed well in research. It means that genes influenced the growth of the embryo of a chicken. But still, even though a chicken gene was predetermined, the genetic engineering of chicken insemination to produce a superior breed with a fast-growing rate of the embryo could be used. Conclusions It could be stated that disease, especially research about the virus is one of the main determinants that could affect the growth of the embryo of broiler chicken.
Librarian: A quality control tool to analyse sequencing library compositions
Authors: Vashishtha Kartavya , Gaud Caroline , Andrews Simon , Krueger Christel 
Published: September 2022

Background: Robust analysis of DNA sequencing data needs to include a set of quality control steps to ensure that technical bias is kept to a minimum. A metric easily obtained is the frequency of each of the nucleobases for each position across all sequencing reads. Here, we explore the differences in nucleobase compositions of various library types produced by standard experimental methodologies. Methods: We obtained the compositions of nearly 3000 publicly available datasets and subjected them to Uniform Manifold Approximation and Projection (UMAP) dimensionality reduction for a two-dimensional representation of their composition characteristics. Results: We find that most library types result in a specific composition profile. We use this to give an estimate of how strongly the composition of a test library resembles the profiles of previously published libraries, and how likely the test sample is to be of a particular type. We introduce Librarian, a user-friendly web application and command line tool which enables checking base compositions of test libraries against known library types. Conclusions: Library preparation methods strongly influence the per position nucleobase content. By comparing test libraries to a database of previously published library types we can make predictions regarding the library preparation method. Librarian is a user-friendly tool to access this information for quality assurance purposes as discrepancies can flag potential irregularities very early on.
Struktury narracyjne w edukacji z perspektywy etycznej
Authors: Jemioł Maciej 
Published: September 2022

Celem niniejszego opracowania jest przyjrzenie się temu, w jaki sposób rozmaite dotyczące edukacji problemy natury etycznej powiązane są ze strukturami narracyjnymi, które występują w procesach edukacyjnych. Wywód, oparty o uwagi m.in. Michaela Oakeshotta, Erika H. Eriksona, Richarda Pringa i Arthura C. Danto dotyczy zwłaszcza trzech rodzajów takich narracji: instrumentalnych, obiektywnych i podmiotowych. Narracje instrumentalne, czyli te wykorzystywane przez nauczycieli w procesach dydaktycznych (np. bajki, anegdoty) przywołane są tu w kontekście problemu reprezentacji marginalizowanych grup w systemie szkolnictwa. Narracje obiektywne, dotyczące świata i funkcjonujących w nim systemów społecznych, omawia się tutaj w odniesieniu do zasady moralnej Eriksona, która głosi, że należy czynić drugiemu to, co przyczyni się do jego rozwoju w tej samej mierze w jakiej przyczyni się do naszego. Narracje podmiotowe, a więc te, które opowiadają sobie sami uczniowie i nauczyciele i które dotyczą ich życia, ukazane są w świetle potrzeby moralnego wychowania młodzieży. W każdym z tym przypadków odsłania się obraz perspektywy struktur narracyjnych jako dobrego formalnego odniesienia dla debat nad moralnymi problemami edukacji.
Passenger-Centric Integrated Airline Schedule and Aircraft Recovery
Authors: Cadarso Luis , Vaze Vikrant 
Published: September 2022

Airlines are known to compete for passengers, and airline profitability heavily depends on the ability to estimate passenger demand, which in turn depends on flight schedules, fares, and the number of seats available at each fare, across all airlines. Interestingly, such competitive interactions and passenger substitution effects may not be limited to the planning stages. Existing regulations in some countries and regions impose monetary compensations to passengers in case of disruptions, altering the way they perceive the utility of other travel alternatives after the disruption starts. These passenger rights regulations may act as catalysts of passengers’ response to recovered schedules. Ignoring such passenger response behavior under operational disruptions may lead airlines to develop subpar recovery schedules. We develop a passenger response model and embed it into a novel integrated optimization approach that recovers airline schedules, aircraft, and passenger itineraries while endogenizing the impacts of airlines' decisions on passenger compensation and passenger response. We also develop an original solution approach, involving exact linearization of the nonlinear passenger cost terms, combined with delayed constraint generation for ensuring aircraft maintenance feasibility and an acceleration technique that penalizes deviations from planned schedules. Computational results on real-world problem instances from two major European airlines are reported, for scenarios involving disruptions, such as delayed flights, airport closures, and unexpected grounding of aircraft. Our approach is found to be tractable and scalable, producing solutions that are superior to airline’s actual decisions and highly robust in the face of passenger response uncertainty. Of particular relevance to the practitioners, our simulation results highlight that accounting for passengers’ disruption response behaviors, even in a highly approximate manner, yields significant benefits to the airline compared with not accounting for them at all, which is the current state-of-the-art. Funding: This work was supported by the Agencia Estatal de Investigación [Grant PID2020-112967GB-C33], the Ministerio de Economía y Competitividad, Spain [Grant TRA2016-76914-C3-3-P], and the Ministerio de Ciencia, Innovación y Universidades, Spain [Grant CAS19/00036].