bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial neural networks in cardiology: analysis of graphic data
Authors: Onishchenko P. S. , Klyshnikov K. Yu. , Ovcharenko E. A. 
Published: January 2022

Aim. To consider application of convolutional neural networks for processing medical images in various fields of cardiology and cardiac surgery using publications from 2016 to 2019 as an example.Materials and methods. In the study, we used the following scientific databases: PubMed Central, ArXiv, ResearchGate. The cited publications were grouped by the area of interest (heart, aorta, carotid arteries).Results. The general principle of work of the technology under consideration was described, the results were shown, and the main areas of application of this technology in the studies under consideration were described. For most of the studies, sample sizes were given. The author’s view on the development of convolutional neural networks in medicine was presented and some limiting factors for their distribution were listed.Conclusion. A brief overview shows possible areas of application of convolutional neural networks in the fields of cardiology and cardiac surgery. Without denying the existing problems, this type of artificial neural networks may help many doctors and researchers in the future.
The association between parameters of oral mucosal immunity and 25-hydroxyvitamin D in patients with rampant caries
Authors: Putneva A. S. , Karavaeva T. M. , Maksimenya M. V. , Tereshkov P. P. , Mishchenko M. N. , Fefelova E. V. , Tsybikov N. N. , Parshina A. A. 
Published: January 2022

Aim. To determine the saliva level of immunoregulatory proteins in patients with rampant caries and 25-hydroxyvitamin D (25(OH)D) deficiency and evaluate the association of their concentration with 25(OH)D plasma level.Materials and methods. The study was performed in two groups. The experimental group included 15 patients aged 20–22 years with rampant caries and the 25(OH)D plasma level of < 20 ng / ml. The control group encompassed 15 healthy age-matched volunteers with the 25(OH)D plasma level of 20–100 ng / ml. The concentrations of B7.2 (CD86), free active TGF-β1, CTLA-4, PD-1, Tim-3, LAG-3, IGFBP-4, and ICAM-1 were assessed using flow cytometry. The levels of LL-37 and secretory immunoglobulin A (sIgA) were measured using ELISA. The Spearman’s rank correlation coefficient was used to reveal a correlation between the indicated proteins and the 25(OH)D plasma level.Results. A decrease in B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevation of IGFBP-4 and ICAM-1 saliva levels were detected in patients with rampant caries and 25-hydroxyvitamin D deficiency. A positive Spearman’s rank correlation coefficient was revealed between plasma 25(OH)D and saliva levels of free active TGF-β1, CTLA4, B7.2 (CD86), LL-37, and sIgA. A negative correlation was revealed between 25(OH)Dand ICAM-1.Conclusion. 25(OH)D deficiency in patients with rampant caries is associated with decreased levels of B7.2 (CD86), PD-1, Tim-3, sIgA, and LL-37 and elevated levels of IGFBP-4 and ICAM-1 in the saliva.
Features of morphometric parameters of vessels in the human portal venous system identified by multislice computed tomography
Authors: Russkikh A. N. , Shabokha A. D. , Tyumentsev N. V. , Derevtsova S. N. 
Published: January 2022

The aim of this study was to identify the morphometric features of the human portal venous system by means of multislice computed tomography (MSCT).Materials and methods. A contrast X-ray study of the portal vein was carried out in 53 men who were treated in the surgical departments of the Krasnoyarsk Regional Hospital No. 1. The average age of the patients was 54.9 ± 1.7 years (36–71 years). Measurements were performed on 3D models of the vascular bed in the portal venous system (GE Advantage Workstation and Siemens singo.via workstations). Branching patterns, length, diameter, angle of the portal vein formation relative to the midline of the human body, and angles of formation of the vessels forming the portal vein in the frontal plane were evaluated.Results. Variations in the morphometric parameters of the intrahepatic vessels of the portal vein are obvious, although the branching patterns are not diverse and are reduced to one type – the magistral pattern (according to V.N. Shevkunenko). The veins that form the portal vein are represented by three systems, each of which has a stem and tributaries that differ in branching patterns and other morphological characteristics.Conclusion. The findings of the study made it possible to supplement the scientific materials regarding branching patterns and morphological characteristics of the portal vein and its tributaries as well as to use the morphometric characteristics of the superior and inferior mesenteric and splenic veins to resolve the issues of surgical intervention on the abdominal organs.
Comparison of cutting and coagulation properties of 1.56 and 1.94 µm fiber lasers and a 0.98 µm semiconductor laser
Authors: Ryabova M. A. , Ulupov M. Yu. , Shumilova N. A. , Portnov G. V. , Tikhomirova E. K. , Malkova M. E. 
Published: January 2022

Aim of the study was to compare the cutting and coagulation properties of 1.56 and 1.94 μm fiber lasers with those of a 0.98 μm semiconductor laser.Materials and methods. A comparative study of the biological effects of 1.56 and 1.94 µm lasers and a 0.98 µm semiconductor laser used in a constant, continuous mode was carried out. The cutting properties of the lasers were evaluated on the chicken muscle tissue samples by the width and depth of the ablation zone formed via a linear laser incision at a speed of 2 mm/s, while the coagulation properties were assessed by the width of the lateral coagulation zone. The zones were measured using a surgical microscope and a calibration slide. For statistical analysis, power values of 3, 5, 7, 9, and 11 W were chosen for each laser wavelength.Results. Analysis of the findings confirmed that laser wavelength had a statistically significant effect on the linear dependence between incision parameters and laser power. It was found that the 1.56 μm fiber laser (water absorption) had a greater coagulation ability but a comparable cutting ability compared with the 0.98 μm laser (hemoglobin absorption). When used in the power mode of 7W or higher, the 1.94 µm laser provided superior cutting performance compared with the 0.98 µm semiconductor laser at the same exposure power. Elevating the power in any of the lasers primarily increased the width of the ablation zone, and to a lesser extent – the crater depth and the width of the lateral coagulation zone. Therefore, in comparison with the 0.98 μm semiconductor laser, higher radiation power in the 1.56 and 1.94 μm lasers mainly influences their cutting properties, expanding the width and depth of the ablation zone, and has a smaller effect on their coagulation ability.Conclusion. The findings of the study showed that the 1.56 and 1.94 μm fiber lasers have better coagulation properties in comparison with the 0.98 μm semiconductor laser. was statistically proven that all incision characteristics (width of the lateral coagulation zone, depth and width of the ablation zone) for the 1.56, 1.94, and 0.98 μm lasers depend on the power of laser radiation. The 1.94 µm laser is superior to the 0.98 µm laser in its cutting properties.
The role of protein kinase C and PI3-kinase in the mechanism of the cardioprotective effect of remote ischemic postconditioning
Authors: Mukhomedzyanov A. V. , Naryzhnaya N. V. , Maslov L. N. 
Published: January 2022

Background. Acute myocardial infarction (AMI) with ST segment elevation is associated with high incidence of complications. Mortality from AMI is about 5%, which has not decreased in recent years. Revascularization provides recovery of coronary blood flow, but also contributes to the occurrence of reperfusion injury to the heart. Remote ischemic postconditioning (RIPostC) is a promising, non-invasive method that can effectively and safely reduce the infarct size.The aim of the study was to investigate the role of protein kinase C and PI3-kinase in the development of the infarct-limiting effect of remote ischemic postconditioning.Materials and methods. The study was performed on Wistar rats. Coronary artery occlusion (45 min) and reperfusion (2 h) were performed. The infarct size (IS) and the size of area at risk (AAR) were assessed. RIPostC was modeled by applying tourniquets to the hind limbs in the hip joint immediately after the restoration of coronary blood flow. All inhibitors were administered intravenously 10 min before reperfusion.Results. In the control group, the IS / AAR ratio was 44%. RIPostC reduced the IS / AAR ratio by about 50%. Preliminary administration of the protein kinase C inhibitor chelerythrine and the PI3-kinase inhibitor wortmannin eliminated the cardioprotective effect of RIPostC.Conclusion. The mechanism of the infarct-limiting effect of RIPostC is implemented through activation of protein kinase C and PI3-kinase.
The effect of coal-derived humic substances and their silver-containing bionanocomposites on arginine balance in peritoneal macrophages of intact mice
Authors: Trofimova E. S. , Zykova M. V. , Danilets M. G. , Ligacheva A. A. , Sherstoboev E. Yu. , Grigorieva I. O. , Mikhalev D. A. , Tsupko A. V. , Logvinova L. A. , Perminova I. V. , Belousov M. V. 
Published: January 2022

Background. Antigen-presenting cells (APCs), especially macrophages, play an important role in the body defense against various pathogens. Their dysfunction and polarization are associated with most inflammatory and autoimmune diseases. The inflammatory process is regulated by activation and / or inhibition of genes differentially expressed by macrophages. Successful correction of inflammation leads firstly to elimination of inflammatory stimuli and then to remodeling and restoration of tissues and organs. It was experimentally confirmed that silvercontaining bionanocomposites based on natural humic substances (HS) obtained from coal of different origin, as well as initial matrices of these HS, are capable of activating pro- and anti-inflammatory properties of macrophages.Aim. To study cytotoxic, pyrogenic, and immunomodulatory properties (arginine balance) of initial HS samples and samples of silver nanoparticles ultradispersed in these HS matrices (HS-AgNPs) in the cell culture of peritoneal macrophages, as well as their effect on pro- and anti-inflammatory properties of APCs.Materials and methods. Cultural and biochemical methods were used in the study.Results. The study showed that the samples CHE-K, CHE-AgNPs, CHS-K, and CHP-K increased M1 macrophage polarization due to stimulation of the NO-synthase activity and inhibition of arginase. The samples CHI-K, CHIAgNPs, CHP-AgNPs, and CHS-AgNPs modulated an alternative M2 or M2-like state of macrophage activation. At the same time, HS are not cytotoxic at effective concentrations, and three out of four studied samples did not contain pyrogenic impurities.Conclusion. The use of HS and their silver-containing bionanocomposites, which have the ability to greatly affect the polarization of antigen-presenting cells, is a promising research area in correction of the inflammatory response for solving an important social and medical problem of treating chronic wounds.
Features of the level of matrix metalloproteinase-2, -3, -9 and tissue inhibitors of metalloproteinases-1, -2, -3, -4 in the aqueous humor of patients with primary open-angle glaucoma
Authors: Chernykh V. V. , Konenkov V. I. , Ermakova O. V. , Orlov N. B. , Trunov A. N. 
Published: January 2022

Aim. To study the content of matrix metalloproteinase (MMP)-2, -3, -9 and tissue inhibitors of metalloproteinases (TIMPs) -1, -2, -3, -4 in the aqueous humor of patients with moderate primary open-angle glaucoma (POAG).Materials and methods. The experimental group included 47 patients with verified moderate primary open-angle glaucoma. The control group consisted of 26 patients with uncomplicated cataract. The levels of MMP-2, -3, -9 were determined with Luminex Performance Human MMP Magnetic Panel 3-plex kit (R&D Systems, USA), the concentration of TIMPs-1, -2, -3, - 4 was determined with the Human TIMP Magnetic Luminex Performance Assay 4-plex kit (R&D Systems, USA). The study was carried out using flow-through field fluorometry on a Bio-Plex 200 double-beam laser analyzer (Bio-Rad, USA).Results. The study showed a statistically significant increase in the levels of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinases-1, -2, -3, -4 in the aqueous humor of patients with moderate POAG compared with patients with uncomplicated cataract.Conclusion. The obtained data on high concentrations and imbalance in the levels of matrix metalloproteinases and their tissue inhibitors in the aqueous humor of patients with moderate POAG confirm the role of local inflammation, as well as impairments in the structure of the extracellular matrix and its remodeling in the mechanisms of development of this pathology.
IDENTIFIKASI NEMATODA USUS GOLONGAN STH (Soil Transmitted Helimnth) MENGGUNAKAN EKSTRAK DAUN JATI (Tectona grandis)
Authors: Khatimah Husnul , Hasanuddin AR Pratiwi , Amirullah Amirullah 
Published: January 2022

Indonesia merupakan salah satu Negara dengan kasus kecacingan yang cukup tinggi. Kasus kecacingan ini disebabkan oleh infeksi cacing usus yang proses menularnya melalui perantara tanah (Soil Transmitted helmint). Penyakit kecacingan banyak ditemukan di daerah dengan kelembaban tinggi terutama pada kelompok masyarakat dengan kebersihan diri dan keadaan lingkungan yang kurang baik. Cacing yang merupakan penyebab dari infeksi adalah Ascaris lumbricoides, Trichuris trichiura dan cacing tambang. Infeksi cacing ini mengakibatkan beberapa masalah yakni gangguan pencernaan, penyerapan serta metabolisme makanan sehingga protein, karbohidrat, lemak, vitamin dan darah dapat hilang dalam jumlah yang banyak. Eosin 2% merupakan pewarna yang selama ini digunakan dalam pewarnaan telur cacing nematode usus. Daun jati (Tectona grandis) merupakan tanaman yang memberikan warna alami karena mengandung.pigmen Antosianin. Antosianin mampu memberikan warna merah, biru, orange atau ungu. Tujuan dari penelitian ini adalah yaitu untuk mengetahui gambaran cacing STH (Soil Transmitted Helminth) menggunakan ekstrak daun jati (Tectona.grandis). Penelitian ini merupakan penelitian dekskriptif dengan pemeriksaan cacing menggunakan metode sedimentasi yang dilakukan dengan pewarna ekstrak daun jati dengan pelarut ethanol dan esoin 2% sebagai kontrol positif. Hasil penelitian menujukkan bahwa ekstrak daun jati konsentrasi 60% lebih baik dan mampu dijadikan sebagai pewarna pengganti eosin. Hal ini dapat disimpulkan bahwa ekstrak daun jati dapat digunakan sebagai pewarna alami dalam pemeriksaan telur cacing.
Kata Kunci: Daun jati, Eosin, Soil Transmitted Helminth, Telur cacing
KEANEKARAGAMAN AVIFAUNA DAERAH TERBUKA DAN TERTUTUP DI WILAYAH KAMPUS KENTINGAN UNIVERSITAS SEBELAS MARET
Authors: Sari Dewi Puspita , Lestari Dwi Indah , Saputra Alanindra , Prabowo Chandra Adi , Harlita Harlita 
Published: January 2022

Perubahan kondisi lingkungan akibat pembangunan sarana dan prasarana akan mempengaruhi kehidupan bagi beberapa organisme termasuk burung. Penelitian ini bertujuan untuk membedakan keanekaragaman burung pada dua habitat yang berbeda di lingkungan kampus UNS sehingga data yang dihasilkan memberi gambaran kualitas daya dukung lingkungan UNS. Penelitian ini dilaksanakan pada bulan April - Mei 2021 di Danau Fakultas Pertanian (FP) UNS dan kawasan Hutan Fakultas Kedokteran (FK) UNS. Subjek penelitian merupakan burung-burung yang terdapat di kedua lokasi. Teknik pengumpulan data menggunakan metode pointcount yakni mengamati subjek penelitian dengan diam di suatu tempat dengan batasan jarak tertentu. Analisis data menggunakan deskripsi kualitatif untuk menguraikan jenis spesies, dan analisis secara kuantitatif untuk mengetahui indeks keanekaragaman jenis, indeks kelimpahan jenis, dan indeks dominansi. Prosedur penelitian dilakukan mulai dari penentuan lokasi, penentuan transek, penentuan titik hitung, pengamatan, akumulasi data, analisis data, dan terakhir kesimpulan. Hasil penelitian menunjukkan tidak terdapat perbedaan nyata keanekaragaman jenis burung dari kedua lingkungan yang diamati. Danau FP UNS memiliki keanekaragaman sebesar 2,016 sedangkan Hutan FK sebesar 2,086. Kategori keanekaragaman tergolong sedang sehingga dapat disimpulkan daya dukung Danau FP dan Hutan FK UNS cukup dengan kestabilan ekosistem yang cukup dan tekanan ekologis rendah.
Determination of Fractional Chromatic Numbers in the Operation of Adding Two Different Graphs
Authors: Sesa Junianto , Siswanto Siswanto 
Published: January 2022

The development of graph theory has provided many new pieces of knowledge, one of them is graph color. Where the application is spread in various fields such as the coding index theory. Fractional coloring is multiple coloring at points with different colors where the adjoining point has a different color. The operation in the graph is known as the sum operation. Point coloring can be applied to graphs where the result of operations is from several special graphs. In this case, the graph summation results of the path graph and the cycle graph will produce the same fractional chromatic number as the sum of the fractional chromatic numbers of each graph before it is operated.
Pengaruh Kompetensi Profesional dan Pedagogik terhadap Kinerja Guru
Authors: Suparti Tinuk , Aziz Al Mubarok Ahmad Aly Syukron 
Published: January 2022

Mutu pendidikan bisa dicapai melalui beberpa komponen, diantaranya: kompetensi profesional dan kompetensi pedagogik, komponen tersebut membutuhkan kinerja dan keahlian guru dalam memberikan dan mengimplementasikan kinerja agar mencapai hasil yang maximal. Penlitian ini bertujuan untuk menelaa pengaruh kompetnsi profesional dan pedagogik terhadap kinerja guru.Teknik analisis data menggunakan metode Quantitative Research (Riset Kuantitatif). Teknik pengumpulan memakai data Observasi, angket, interview dan dokumentasi. Populasi penelitian yaitu seluruh guru Raudhatul Athfal di Kecamatan Prigen Kabupaten Pasuruan yang berjumlah 65 guru. hasil penelitian diketahui bahwa: terdapat pengaruh positif secara simultan terhadap kinerja guru Raudhatul Athfal di Kecamatan Prigen Kabupaten Pasuruan. Hal ini dapat dibuktikan dari hasil penelitian secara empirik diketahui nilai Fhitung = 19.420 dan f tabel sebesar 4,00 sehingga diketahui (Fhitung Ftabel, 19,420 4,00) , dan p-value = 0,000 0,05. Dengan demikian pengaruh kompetensi profesional dan pedagogik secara simultan terhadap kinerja guru adalah signifikan. Maka diperoleh kesimpulan bahwa hipotesis kerja (Ha) yang berisi “ Kompetensi profesional dan pedagogik secara simultan memiliki pengaruh yang signifikan terhadap kinerja guru Raudhatul Athfal di Kecamatan Prigen Kabupaten Pasuruan” dapat diterima atau terbukti kebenarannya.
Pengaruh Media Color Bead Stairs Terhadap Kemmapuan Berhitung Anak Usia 4-5 Tahun
Authors: Dini Rahmi Meilisti , Sayekti Tri , Kusumawardani Ratih 
Published: January 2022

@font-face{ font-family:"Times New Roman"; }@font-face{ font-family:"宋体"; }@font-face{ font-family:"SimSun"; }@font-face{ font-family:"Calibri"; }@font-face{ font-family:"Times New Roman Italic"; }@font-face{ font-family:"Arial"; }p.MsoNormal{ mso-style-name:Normal; mso-style-parent:""; margin-bottom:10.0000pt; margin-left:-0.0500pt; mso-para-margin-left:-0.0100gd; text-indent:-0.0500pt; mso-char-indent-count:-0.0100; mso-hyphenate:none; mso-outline-level:1; vertical-align:top; line-height:114%; font-family:Calibri; font-size:11.0000pt; position:relative; top:0.5000pt; mso-text-raise:-0.5000pt; }p.MsoFooter{ mso-style-name:Footer; margin-bottom:0.0000pt; margin-left:-0.0500pt; mso-para-margin-left:-0.0100gd; text-indent:-0.0500pt; mso-char-indent-count:-0.0100; mso-hyphenate:none; mso-outline-level:1; vertical-align:top; font-family:Calibri; font-size:11.0000pt; position:relative; top:0.5000pt; mso-text-raise:-0.5000pt; }p.MsoHeader{ mso-style-name:Header; margin-bottom:0.0000pt; margin-left:-0.0500pt; mso-para-margin-left:-0.0100gd; text-indent:-0.0500pt; mso-char-indent-count:-0.0100; mso-hyphenate:none; mso-outline-level:1; vertical-align:top; font-family:Calibri; font-size:11.0000pt; position:relative; top:0.5000pt; mso-text-raise:-0.5000pt; }span.msoIns{ mso-style-type:export-only; mso-style-name:""; text-decoration:underline; text-underline:single; color:blue; }span.msoDel{ mso-style-type:export-only; mso-style-name:""; text-decoration:line-through; color:red; }div.Section0{page:Section0;}Tujuan dari penelitian ini untuk mengetahui pengaruh penggunaan media color bead stairs terhadap kemampuan berhitung anak usia 4-5 tahun di RA Al-Layyinah Tangerang. Subjek riset ini terdapat 21 anak. Penelitian ini memakai pendekaan kuantitatif melalui metode eksperimen dengan desain true eksperimental design dengan desain Pretest-posttest Control Group Design. Teknik pengumpulan data yg dipakai adalah observasi serta dokumentasi. Tehnik analisis data berupa statistik non parametrik dengan uji jenjang Kolmogrov Smirnov dengan data hasil penilaian pretest serta posttest. Hasil penelitian ini menunjukkan bahwa rata-rata kemampuan berhitung anak kls eksperimen pretest sebesar 0,064 melainkan pada kelas eksperimen posttest sebesar 0,200 meningkat sebesar 136% dan untuk kelas control pretest sebesar 0,086 sedangkan pada kelas kontrol posttest sebesar 0,129 maka dapat disimpulkan terjadi peningkatan sebesar 43% bahwa ada pengaruh signifikan penggunaan media color bead stairs terhadap kemampuan berhitung anak usia 4-5 tahun. Sehingga diketahui bahwa ada pengaruh media color bead stairs terhadap kemampuan berhitung anak usia 4-5 tahun di RA Al-Layyinah Tangerang-Banten.
One for All and All in One: Modified Silica Kit-based Protocol for simultaneous sample-specific Extraction of DNA from a Variety of Source Materials
Authors: Schmerer Wera M 
Published: January 2022

Abstract
Protocols utilized for the extraction of DNA vary significantly with regards to steps involved and duration of the overall procedure due to material-specific requirements for ensuring the highest possible yield in recovery of DNA. This variation mostly affects aspects of sample preparation and digestion steps required to release the DNA from the sample material.In contexts such as the development of new PCR-based assays - which always includes a test of species-specificity - reference samples from a number of species are utilized, requiring extraction of DNA from a variety of source materials, each with their specific conditions for effective isolation of DNA.The method presented here follows the strategy of synchronizing sample material-specific aspects such as sample preparation and digestion in such a way that one common protocol can be utilized for the actual extraction and purification of the DNA, allowing for an overall more efficient extraction process, while maintaining optimized conditions for DNA recovery.