bluuebunny/crossref_metadata_2025_split huggingface dataset:
TIPE2 reverses TGF-β1-induced migration and invasion by targeting the Smad2-EMT signal pathway in ovarian cancer cells
Authors: Tang Zhongyun , Zhang Derui , Yao Chenchen , Jiang Mengmeng , Wang Chongli , Jia Mingyue , Yu Huayun , Xue Chenyue , Liu Yuqiu , Shi Yongyu , Zhang Lining , Wang Xiaoyan , Wei Zengtao 
Published: June 2022

Abstract
Ovarian cancer is the deadliest among all the gynecologic malignancies, and it is also a kind of highly metastatic disease. Transforming growth factor-β1 (TGF-β1) is a dominant driver of epithelial-mesenchymal transformation (EMT) and promotes the metastasis of tumor cells and therapy resistance. Tumor necrosis factor-α-induced protein 8 (TNFAIP8)-like 2 (TIPE2) is a negative regulator of innate and adaptive immunity. It also involves the occurrence and development of different tumors. However, the roles of TIPE2 in ovarian cancer and whether TIPE2 reverses TGF-β1-induced EMT in ovarian cancer cells remain unclear. The present study demonstrated that TIPE2 protein was significantly down-regulated in ovarian cancer tissues and related to tumor pathological types, patients’age, tumor differentiation degree, and FIGO stage of tumor. TIPE2 inhibited the metastasis and EMT of ovarian cancer cells by combining with Smad2 in vitro and intraperitoneal metastasis model and reversed TGF-β1-induced migration, invasion of ovarian cancer cells by targeting the Smad2-EMT signaling pathway. Moreover, we found that TIPE2 decreased the expression of TGF-β1 and TGF-β1 down-regulated the levels of TIPE2 in ovarian cancer cells. There is a negative relationship between TIPE2 and TGF-β1. In summary, our results suggest TIPE2 plays an important inhibitory role in the metastasis of ovarian cancer by affecting TGF-β1/Smad2/EMT signal pathway and acts as a potential target in the diagnosis and treatment of ovarian cancer.
Cardiovascular Screening of Athletes: Implications in Reducing the Risk of Sudden Cardiac Deaths
Authors: Vaňova Natalia , fatima Ghizal , Zenuch Pavol , Fedacko Jan 
Published: June 2022

Abstract
Sudden cardiac death (SCD) in sports is defined as unexpected death during exercise or shortly after physical exertion (within 24 hours of exercise cessation) in athletes who appeared perfectly healthy otherwise. To highlight the importance of cardiovascular screening in athletes for reducing incidences of SCD, we performed a prospective study and analysed 280 athletes who were recruited and followed up between 2016 to 2021 at the Agel Košice-Šaca Hospital (city and country) for cardiovascular screening using electrocardiography (ECG) and other imaging modalities such as echocardiography (ECHO). During the screening, arrhythmias were diagnosed in thirteen athletes and higher degrees of AV block and ventricular extrasystoles were diagnosed in four athletes. These athletes subsequently underwent interventional procedures. At the end of the follow up period and interventional procedures, three athletes were diagnosed with sever cardiovascular disease (CVD), which led to early termination of their sports career. Our findings suggest that endurance athletes in particular are at risk for arrhythmias and chronic myocardial remodelling and that most of the diseases that are associated with an increased risk of SCD could be identified by changes in resting ECG. We also confirmed that endurance athlete, both professional and recreational, are at higher risk of developing atrial fibrillation later in life. In addition, we emphasize on the benefit of an automated ECG module and echocardiographic examination, and suggest that SCD prevention in athletes lies in improving and expanding cardiovascular screening.
Evaluation of lung adverse events with trastuzumab using the Japanese pharmacovigilance database
Authors: Kanbayashi Yuko , Uchida Mayako , Kashiwagi Misui , Akiba Hitomi , Shimizu Tadashi 
Published: June 2022

Abstract
The present study aimed to determine the risk of trastuzumab-induced lung toxicity, time to onset, and post hoc outcomes using the Japanese Adverse Drug Event Report database. We analysed data for the period between April 2004 and March 2021. Data on lung toxicities were extracted, and relative risk of adverse events (AEs) was estimated using the reporting odds ratio. We analysed 1,772,494 reports and identified 4,362 reports of AEs caused by trastuzumab. Of these, 693 lung toxicities were reportedly associated with trastuzumab. Signals were detected for seven lung toxicities: interstitial lung disease, pulmonary oedema, pleural effusion, lung disorder, acute pulmonary oedema, pulmonary fibrosis, and radiation pneumonitis. Among these, interstitial lung disease was the most frequently reported (61.8%). A histogram of times to onset showed occurrence from 1 to 105 days, but some cases of interstitial lung disease occurred even more than one year after the start of administration. The AEs showing the highest fatality rates were interstitial lung disease, pulmonary fibrosis, and radiation pneumonitis. This study focused on lung toxicities caused by trastuzumab as post-marketing AEs. Some cases could potentially involve serious outcomes; therefore, patients should be monitored for signs of the onset of these AEs not only at the start of administration, but also over an extended period, especially for interstitial lung disease.
Association between Abnormal Glucose Regulation at Different Time Point and Fetal Congenital Heart Diseases during Pregnancy: a Cohort Study of 72236 Births
Authors: Zhang Yulong , Zhang Qian , Ye Xu , Wu Yi , Lin Tinghua , Yan Jianying , Zheng Xiangqin , Huang Huiyun , Zhang Wenhui , Lin Hai , Lai Shuhua 
Published: June 2022

Abstract
BACKGROUND: GDM or prepregnancy diabetes mellitus during pregnancy will lead to an increased risk of fetal congenital malformations16-18. However, the influence of different time periods on blood glucose level is not clear, the object of this study was to explore the association between abnormal glucose regulation at different time point and congenital heart diseases during pregnancyMETHODS: This retrospective and consecutive cohort study were conducted on singleton pregnancies carried to 20 completed weeks of gestation or beyond, from the Fujian Provincial Maternity and Children’s Hospital, China, between January 1, 2014 and December 31, 2020. A diagnosis of gestational diabetes was defined according to Fasting blood glucose level and 75-g OGTT. Maternal and infant characteristics and outcomes were compared between neonates born to mothers with and without Abnormal blood glucose at different time of OGTT. Descriptive statistics are shown as mean ± standard deviation or median (range interquartile) for continuous variables and tested with ANOVA or Kruskal-Wallis rank-sum test, whereas frequency (percentage) and χ2 test were used for categorical variables. All analyses were conducted using R version 4.1.0.RESULTS: A total of 71703 normal subjects and 533 subjects with fetal cardiovascular malformations were included in this study. Pregnant women who deliver a normal fetus tend to older and have higher rate of gestational hypertension and abnormal glucose during pregnancy than those who deliver a newborn with CHD (P < 0.05). Compared to the corresponding normal group, GDM, OGTT 0 hour≥5.1mmol/L, OGTT 1 hour≥10mmol/L, and OGTT 2 hour≥8.5mmol/L confer to 55%, 48%, 54%, and 59% increase in risk of CHD, respectively. Compared to group 1 (OGTT 0h and OGTT 1h and 2h are both normal), group 3 (OGTT 0h is normal, while OGTT 1h or 2h is abnormal) and group 4 (OGTT 0h and OGTT 1h or 2h are both abnormal) exhibit higher risk of CHD (OR= 1.53 and 2.21, respectively, all P<0.05). However, group 2 (OGTT 0h is abnormal, while OGTT 1h and 2h is normal) did not demonstrate a statistically significant elevated risk.CONCLUSION: Gestational diabetes is an identified hazard factor for neonatal CHD. The effect of GDM on CHD is more reflected in impaired tolerance of glucose regulation than in elevated fasting blood glucose levels. Pregnant women with normal fasting glucose but abnormal OGTT 1 hour or 2 hour may be overlooked as an implicit risk population, and should be given adequate attention.
The Inhibitory Effect of Betulinic Acid on Epithelial-Mesenchymal Transition Pathway in Renal Cell Carcinoma
Authors: Ataş Merve Nur , Ertuğrul Barış , İplik Elif Sinem , Çakmakoğlu Bedia , Ergen Arzu 
Published: June 2022

Abstract
Renal cancer is the most lethal urological cancer and characterized by high metastasis rate at initial diagnosis and drug resistance to current chemotherapeutics. Betulinic acid is a pentacyclic triterpene with broad biological activity that occurs naturally in variety of plants. Even though the anti-cancer efficacy of betulinic acid have been reported by many studies, the information about the pathways and the molecules which are affected by betulinic acid in renal cancer are limited. Epithelial-mesenchymal transition (EMT) is considered as the initial step of metastasis and contributes to drug resistance of cancer cells. Depending on the role of EMT in cancer progression and drug resistance, targeting EMT may represent an effective strategy in this context. Therefore, we aimed to investigate the anti-metastatic effects of betulinic acid on renal cell carcinoma cells by evaluating two EMT markers, SNAIL-1, and SDC-2. Following the treatment of betulinic acid at determined doses by WST-1 cytotoxicity assay in our previous study, SDC-2 expression level was decreased in both cell lines. Additionally, in correlation with this result, we also found a reduction in SDC-2 and SNAIL-1 protein levels which are measured by ELISA. Furthermore, the migration and invasion capacities were suppressed by betulinic acid treatment in metastatic renal adenocarcinoma ACHN cells. Taken together, our findings indicate that betulinic acid may constitute a potential treatment approach for renal cancer with further investigations.
Clinical features and serum cytokine profiles of elderly-onset adult-onset Still’s disease: comparison with young and middle-aged-onset cases
Authors: Yagishita Mizuki , Tsuboi Hiroto , Kuroda Yuki , Sawabe Tomoki , Kawashima Akira , Kawashima Fumina , Uematsu Nana , Sato Ryota , Nishiyama Taihei , Terasaki Mayu , Toko Hirofumi , Honda Fumika , Ohyama Ayako , Abe Saori , Kitada Ayako , Miki Haruka , Hagiwara Shinya , Kondo Yuya , Sumida Takayuki , Matsumoto Isao 
Published: June 2022

Abstract
Recent studies have suggested that the clinical features of elderly-onset adult-onset Still’s disease (AOSD) differ from those of young and middle-aged-onset patients, whereas the details remain unclear, and cytokine profiles of elderly-onset AOSD have not been reported. To clarify the clinical features and cytokine profiles of elderly-onset AOSD, we examined patients with AOSD who developed the disease between January 2006 and September 2021. We divided the patients into the young and middle-aged-onset group (aged <65 years) and the elderly-onset group (aged ≥65 years) and compared the groups in terms of patient characteristics, clinical symptoms, laboratory findings including serum interleukin (IL)-6 and IL-18, treatment, and prognosis. A total of 48 patients were examined (10 in the elderly-onset group). In the elderly-onset group, atypical rash was significantly more frequent, typical rash and splenomegaly were significantly less frequent, white blood cell count and neutrophil ratio were significantly higher and serum IL-6 levels were significantly lower. Serum IL-6 showed a significantly negative correlation with age at onset. Treatment and relapse were comparable between the 2 groups, whereas infections were significantly more frequent in the elderly-onset group. The clinical features and cytokine profiles of elderly-onset AOSD might differ from those of young and middle-aged-onset AOSD.
Logic model for opioid analgesics in chronic non-malignant pain management, an in-depth qualitative methods study
Authors: Iqbal Ayesha , Knaggs Roger David , Anderson Claire , Toh Li Shean 
Published: June 2022

Abstract
BackgroundOpioids are commonly used for the management of chronic non-malignant pain in Pakistan; but there is a lack of literature around precursors or motivators in the use of opioids.AimThis study holistically explored factors contributing towards the unsafe use of opioids and identifies strategies to overcome them.MethodThis study used exploratory qualitative methods using interviews, focus groups and non-participant observational case studies. Interviews and focus groups were carried out face to face as well as virtually whereas non-participant observations were conducted in community pharmacies in Islamabad and Khyber Pukhtoon Khuwa province, Pakistan. Data was collected from 4 stakeholder groups; pharmacy policy makers (n = 11), people with chronic non-malignant pain (n = 14), doctors (n = 31) and community pharmacists (n = 36) by purposive critical case sampling method. Data were inductively analysed using reflexive thematic analysis and then deductively mapped to a social ecological framework. Non-participant observations were analysed using a cross case synthesis using explanation building technique. Data from all 3 methods was triangulated to develop a logic model.ResultsIdentified factors at macro (regulation), meso (social perceptions of pain and opioids) and micro levels (uncontrolled pain, self-medication, health literacy) are presented holistically were used to develop a logic model for the prevention and mitigation of factors currently causing unsafe use of opioids.ConclusionsPolicymakers need to contextualise factors and carefully plan community pharmacy interventions within healthcare system to overcome challenges and barriers on various social ecological levels to promote optimised use of opioids in the management of chronic non-malignant pain in Pakistan.
Comprehensive Analysis of Pyroptosis-related Genes for the Prognosis of Esophageal Cancer
Authors: Zhou Xiaoying , Li Wenjie , Xia Peichen , Chen Han , Su Xin , Li Xinyan , Jiang Xingzhou , Zhang Guoxin 
Published: June 2022

Abstract
Esophageal cancer (EC), known for its high malignancy and difficulty in treatment, has seen a significant increase in incidence in recent years. Studies on prognosis of EC are extremely urgent to provide personalized medicine which will benefit more patients individually. Pyroptosis, an inflammatory form of programmed cell death triggered by caspase-1/4/5/11 which is activated by some inflammasomes has been connected to cancer in recent studies. However, the association between pyroptosis-related genes and esophageal cancer has not been elucidated, and their vital role in the development and prognosis of esophageal cancer needs further research. In this study, we obtained 290 samples from both TCGA and GEO database. Firstly, we screened 32 pyroptosis-related regulators that were differentially expressed in normal esophageal and cancer tissues. Then, univariate Cox regression analysis and LASSO analysis were applied and we obtained 8 key pyroptosis-related genes to construct the risk model for predicting the prognosis of EC patients. We verified the expression levels of these eight genes in cells in vitro. Based on the risk score and other clinical features, a nomogram was plotted. Through two clustering analyses, we elaborated the enrichment of pyroptosis-related genes in terms of pathways, signaling, immune response, and clinical characteristics. Finally, we calculated the pyroptosis score which could also predict the prognosis of EC and had strong relationship with clinical features. In conclusion, pyroptosis-related genes are intimately related with EC and can be used to predict the prognosis of EC, which may also provide novel therapeutic schedule for EC patients.
Urban Greenness and Pricing Premium on Residential Properties
Authors: Wahid Abdul , Mumtaz Muhammad Zubair , Kowalewski Oskar 
Published: June 2022

Abstract
This study examines how the urban climate affects the price, and urban greenness causes the pricing premium. To explore the greenness aspects, we construct a composite pollution index for each property traded in twin cities—Islamabad and Rawalpindi—based on the area traded properties' water, air, noise, and soil pollution. To examine the pricing efficiency of these properties, we categorize our sample into green and non-green properties using machine learning and measure the impact of environmental factors on their price. Along with other property characteristics, we observe that air pollution, noise pollution, and water pollution emerge as vital factors that define the prices of properties. Similarly, investors are willing to pay higher prices for green residential properties with a lower pollution index. The study findings are helpful for investors, housing society managers and developers, as they show that greenness pays back.JEL Codes: R20, R30, R31
Comprehensive treatment of a rare primary malignant pericardial mesothelioma: A case report and literature review
Authors: Liu Chunyu , Zhao Bin , Cheng Weiwei , Ma Dongliang , Wei Xing , Zhang Shunye 
Published: June 2022

Abstract
Background: Primary malignant pericardial mesothelioma (PMPM) is an extremely rare aggressive malignancy with nonspecific symptoms; thus, cases of PMPM are usually found incidentally. Despite treatment, it is considered extremely fatal.Case presentation: We encountered an asymptomatic 64-year-old woman diagnosed with PMPM by positron emission tomography/computed tomography (PET/CT). After 22 months of supportive treatment and chemotherapy, she presented to our facility with progressive dyspnoea. Surgery was performed. Excision biopsy showed thickening pericardium and many poorly circumscribed masses, and cut sections showed friable grey–white tissue with areas of necrosis. Pathological examination confirmed epithelium-like malignant mesothelioma. The patient was continuously treated with a previously prescribed chemotherapy regimen. The patient did not exhibit dyspnoea recurrence, and PET/CT showed no progression 7 months postoperatively.Conclusion: Comprehensive treatment, including chemotherapy, supportive treatment, and timely surgery, is effective for unresectable PMPM. When patients present with symptoms caused by PMPM that are not relieved by medication, we recommend prioritising urgent surgery.
Preservation of endocrine function after Ommaya reservoir insertion in children with cystic craniopharyngioma
Authors: Lohkamp Laura-Nanna , Kulkarni Abhaya V. , Drake James M. , Rutka James T. , Dirks Peter B. , Taylor Michael , Ibrahim George M. , Hamilton Jill , Bartels Ute K. 
Published: June 2022

Abstract
Introduction: Children with craniopharyngiomas (CP) can experience significant morbidities caused by extensive surgery and/or radiation. Ommaya reservoir insertion (ORI) into cystic CP represents a minimally invasive approach allowing immediate decompression and aims to avoid additional injuries. The purpose of this study was to determine the surgical outcome and relevance of upfront ORI (+/- intracystic treatment) for preservation of endocrine function. Methods: We performed a retrospective chart review of children with CP treated at the Hospital for Sick Children between 01/01/2000 and 15/01/2020. Endocrine function was reviewed at the time of initial surgery and throughout follow-up. New endocrinological deficits related to the index procedure were defined as immediate failure (IF), whereas postoperative duration of endocrinological stability was defined as event-free survival. The rate of IF and EFS were compared between the treatment groups. Results: Seventy-nine patients were included and had a median age of 8.3 years (range 2.1–18.0 years); 31 were males. Fifty-three patients with upfront surgical treatment, including 29 ORI and 24 gross total or partial resections had sufficient endocrinological follow up data. Endocrine dysfunction occurring immediately after the index procedure was observed in 15 patients (62.5%) in the resection group compared to 2 patients (6.8%) in the ORI group, odds ratio: 0.05 (CI: 0.01–0.26, p < 0.0001). Along those with immediate deficits, mean EFS after ORI was 19.4 months (CI: 11.6–34.2), compared to 13.4 months (CI:10.6-NA) after surgical resection. Conclusions: Endocrine function was preserved in patients with upfront ORI (+/- intracystic treatment), which was confirmed as a minimally invasive procedure with an overall low morbidity profile.
Semi-field evaluation of the space spray efficacy of Fludora Co-Max EW against wild insecticide-resistant Aedes aegypti and Culex quinquefasciatus mosquito populations from Abidjan, Côte d’Ivoire
Authors: Zahouli Julien Z. B. , Dibo Jean-Denis , Diakaridia Fofana , Yao Laurence , Souza Sarah D. , Horstmann Sebastian , Koudou Benjamin G. 
Published: June 2022

Abstract
Background: Space spraying of insecticides is still an important mean of controlling Aedes and Culex mosquitoes and arboviral diseases. This study evaluated the space spray efficacy of Fludora Co-Max EW (a combination of flupyradifurone and transfluthrin, with Film Forming Aqueous Spray Technology (FFAST)) against wild, insecticide-resistant Aedes aegypti and Culex quinquefasciatus populations from Abidjan, Côte d’Ivoire, against K-Othrine EC (deltamethrin-only product), through small-scale field trials. Methods: Wild Ae. aegypti and Cx. quinquefasciatus mosquito larvae were collected in Abidjan, Côte d’Ivoire from August to December 2020. Mosquito larvae were reared until adult stage. Emerged adult females were tested against Fludora Co-Max EW and K-Othrine EC using ultra-low volume cold fogging (ULV) and thermal fogging (TF) both outdoors and indoors in Agboville, Côte d’Ivoire. Cages containing 20 mosquitoes each were placed at 10, 25, 50, 75 and 100 m from the spraying line for outdoor spraying, and at ceiling, mid-height and floor levels for indoor house spraying. Knockdown and mortality were recorded at each checkpoint and compared by treatments.Results: Overall, Fludora Co-Max EW induced significantly higher knockdown and mortality effects in the wild insecticide-resistant Ae. aegypti and Cx. quinquefasciatus compared with K-Othrine EC. With both species, Fludora Co-Max EW mortality rates were above 80% (up to 100%) for outdoor ULV spray at each distance checkpoint (i.e. 10 to 100 m), and 100% for indoor ULV and TF sprays at all level checkpoints (i.e. ceiling, mid-height and floor). K-Othrine EC induced high mortality indoors (97.9-100%), whereas outdoor mortality rapidly declined in Ae. aegypti from 96.7% to 36.7% with ULV, and 85.0% to 38.3% with TF, from 10 to 100 m. For outdoor Fludora Co-Max EW spray, ULV showed both higher knockdown and killing performance Ae. aegypti and Cx. quinquefasciatus compared with TF. Fludora Co-Max EW performed better against Cx. quinquefasciatus compared with Ae. aegypti.Conclusions: Fludora Co-Max EW induced high mortality and knockdown effects against wild insecticide-resistant Ae. aegypti and Cx. quinquefasciatus Abidjan strains and performed better than K-Othrine EC. The presence of flupyradifurone and transfluthrin (with new and independent modes of action) and FFAST technology in the current Fludora Co-Max EW formulation appears to have broadened its killing capacity. Fludora Co-Max EW is thus an effective adulticide and may be a useful tool for Aedes and Culex mosquito and arbovirus control in endemic areas.