bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated Opposite Charge Grafting Induced Ionic-junction Fiber
Authors: Xing Yi , Zhou Mingjie , Si Yueguang , Yang Chi-Yuan , Feng Liang-Wen , Wu Qilin , Wang Fei , Wang Xiaomin , Huang Wei , Cheng Yuhua , Zhang Ruilin , Duan Xiaozheng , Sun Hengda , Zhang Jiayi , Jiang Su , Wang Gang , Zhu Meifang 
Published: June 2022

Abstract
The emergence of ionic-junction devices has attracted growing interests for the potential of serving as signal transmission and translation media between electronic devices and biological carrier systems with ions. Among them, fiber-shaped iontronics possesses great advantage in implantable applications owing to the unique one-dimensional geometry, but is restricted to the challenges in the fabrication of fixed cation-anion junction on curved surface. Here, we developed a polyelectrolyte heterojunction-based ionic-junction fiber via an integrated opposite charge grafting process capable of kilometer scale fabrication. The ionic-junction fibers can be integrated to functions such as ionic diodes and ionic bipolar junction transistors, where “0–1” rectification and “on-off” switch of input signals are implemented. Moreover, synaptic functionality has also been demonstrated by utilizing the fiber memory capacitance. Connection between our ionic-junction fiber and sciatic nerve of the mouse simulating end-to-side anastomosis is further performed to realize effective nerve signal conduction, verifying the capability for next-generation artificial neural pathway in implantable bioelectronics.
Preventing Group Privacy DisclosureThrough Synthetic Data: An Evaluation of Recommender System Methods
Authors: Yépez Carolina , Recalde Lorena , Loza-Aguirre Edison 
Published: June 2022

Abstract

Nowadays, both the privacy of the data that feeds information systems and the effects of its improper handling are a matter of concern. Thus, in recommender systems, mitigating the disclosure of user privacy has become an issue of attention given the challenges that this entails. While research on algorithmic strategies to synthesize user ratings has addressed the concealment of user profiles in traditional recommender systems, less work has been done regarding the exposure of preferences and sensitive data in group recommender systems. In this paper, we evaluate two methods used to generate synthetic ratings and how they perform when applied in group recommender systems. Specifically, we apply state-of-the-art privacy-based methods in a group recommender system to introduce synthetic data and to assess whether and to what extent their behavior changes in terms of rating prediction. We performed this by exploring several possible combinations of GRS implementation (strategies used in group creation, size of groups, individual ratings prediction algorithms, preferences on aggregation methods). Our results show that it was possible to hide the information of individual preferences without affecting group recommendation performance while preserving privacy. We also found that CART was the method that synthesized data with the less difference from the original data. We do not find significative influence of strategies for group creation, group size or individual ratings prediction methods. To the best of our knowledge, this work represents the first attempt to use partially synthetic group ratings in GRS.
In silico molecular docking evaluation reveals high potencies of some natural antifungal metabolites on melanin biosynthesis and appressoria formation enzymes in Magnaporthe oryzae
Authors: Khan Md. Arif , Khan Md. Abdullah Al Mamun , Sanjana Jannatul Maowa , Ahsan Asif , Gupta Dipali Rani , Hoque M. Nazmul , Islam Tofazzal 
Published: June 2022

Abstract
Magnaporthe oryzae is one of the most notorious fungal pathogens that causes blast disease in cereals and results in enormous loss of grain production. Many chemical fungicides are being used to control the pathogen but none of them are effective against blast disease. Thus, there is a demand to discover potential and safe natural biofungicides to manage blast disease successfully. To find out effective biofungicides, we performed in silico molecular docking analysis of some natural compounds targeting four enzymes namely, scytalone dehydratase, SDH1 (1STD), trihydroxynaphthalene reductase, 3HNR (YBV1), trehalose-6-phosphate synthase, Tps1 (6JBI) and isocitrate lyase, ICL1 (5E9G) of M. oryzae fungus that regulate melanin biosynthesis or appresorium formation. Thirty-nine natural compounds that previously reported to inhibit the growth of M. oryzae were subjected to rigid and flexible molecular docking against aforementioned enzymes followed by molecular dynamics simulation and free energy analysis of protein-ligand complexes. The results of virtual screening showed that out of 39, 12 compounds showed good binding energy with any one of the target enzymes as compared to reference molecule azoxystrobin and strobilurin. Among the compounds, camptothecin GKK1032A2 and arohynapene-B bind more than one target enzymes of M. oryzae. All the compounds except tricyclazole showed good bioactivity score. Taken together, our results suggest that all of the 12 compounds have the potential to develop new fungicides but camptothecin, GKK1032A2 and arohynapene could act as multi-site mode of action fungicides against M. oryzae.
In vivo emergence of ceftazidime-avibactam resistance and loss of hypermucoviscosity in carbapenem-resistant Klebsiella pneumoniae during antibiotics treatment
Authors: Xu Mengxin , Qian Changrui , Jia Huaiyu , Feng Luozhu , Shi Shiyi , Zhang Ying , Wang Lingbo , Cao Jianming , Zhou Tieli , Zhou Cui 
Published: June 2022

Abstract
Background
Ceftazidime-avibactam (CZA) is currently one of the few effective antibiotics for carbapenem-resistant Enterobacteriaceae (CRE). We characterized in vivo evolution of carbapenem and CZA resistance and decreased hypermucoviscosity (Hmv) by analyzing two longitudinal carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) clinical isolates (FK8578, FK8695) from an ICU patient during antimicrobial treatment.
Methods
Antibiotic susceptibility testing was performed to investigate the change of antibiotics resistance. String test, quantification of capsule, biofilm inhibition test and Galleria mellonella (G. mellonella) infection model were used to investigate the change of Hmv and virulence. Whole genome sequencing (WGS) and comparative genomic analysis were conducted to illustrate the underlying mechanisms.
Results
Compared with FK8578, minimum inhibitory concentrations (MICs) were increased for CZA while decreased for carbapenems antibacterial drugs in FK8695. Virulence results showed that compared with FK8578, FK8695 was negative in the string test, with decreased capsular production, smaller amounts of biofilm formation and higher survival rate of G. mellonella. The results of WGS showed that mutation of blaKPC−2 to blaKPC−33 was responsible for the change of drug resistance phenotype between FK8578 and FK8695. pLVPK-like virulence plasmid without siderophore synthesis operon was identified in the two strains. The single nucleotide deletion in rmpA gene of FK8695 strain may be responsible for the loss of its Hmv phenotype.
Conclusions
This is the first report to describe the in vivo emergence of CZA resistance and the loss of Hmv in CR-hvKp strains during CZA treatment and illuminate the mechanism of its resistance and virulence changes. There is an urgent need for CZA resistance monitoring and timely adjustment of anti-infective treatment strategies in clinical settings.
Comparison of Pain Scores, Vitreous Reflux and Intraocular Pressure in Patients Who Received Intravitreal Injection with Different Size Needle.
Authors: Boz Ali Altan Ertan , Kocamış Sücattin İlker , Boncukçu Kadriye Demir , Sönmezoğlu Halil İbrahim , Çelik Erkan 
Published: June 2022

Abstract
Purpose
Intravitreal injection (IVI) is a common vitreoretinal procedure, and most of the patients, over the course of their disease, have to multiple IVI. Pain sensations during IVI may reduce the desire to continue the repeated IVI and cause sudden movements of the eye that to occur complications. The aim of this study to compare the effect of needle size on immediate intraocular pressure (IOP), vitreous reflux and patients’ pain experienced after the IVI.
Methods
A hundreds and ten eyes of 110 patients who were firstly administered intravitreal ranibizumab or aflibercept were randomized according to the use of needle size, 30-gauge (Group 1) or 26-gauge (Group 2). The reflux was graded post-IVI procedure. Immediately after the IVI, patients were administered to visual analogue scale (VAS) to assess the pain degree. The IOP measurements were taken 30 minutes after the operation. The average of these scores was used as the primary outcome.
Results
Both groups consisted of 55 patients were included the study. The mean VAS score was 2.18 in group 1 and 4 in the group 2. The reflux grade of the group 1 was also lesser than that of the group 2. IOP was comparable between the groups (26.3 ± 5.66 / 25.4 ± 4.02 mmHg). Groups were divided into subgroups according to the intravitreal agent use, there was not a statistically significant difference in the VAS scores.
Conclusions
Thirty-gauge needle is more comfortable and safer than 26-gauge needle for the IVI.
Metadichol® a nano lipid emulsion that expresses all 48 nuclear receptors in stem and somatic cells
Authors: Raghavan Palayakotai R 
Published: June 2022

Abstract

The conversion of somatic cells back into induced pluripotent stem cells (iPSCs) or embryonic-like stem cells involves the introduction of four genes commonly called Yamanaka factors, i.e., Sox2, Oct4, Klf4, and c-Myc, into the cells. Because these genes and the viral vectors used to introduce them into cells have the potential to cause cancer, iPSC lines are not clinically useful. Most instances of direct reprogramming have been achieved by the forced expression of defined factors using viral vectors. Here, we show that Metadichol® has the potential to generate iPSCs nonvirally and may be helpful in clinical applications. Metadichol is a nanoformulation of long-chain alcohols derived from food. Quantitative real-time PCR (qRT-PCR) and western blotting showed that OCT4, SOX2, and Nanog are expressed when fibroblasts were treated with Metadichol at one picogram to 100 nanograms. Reverse-transcription PCR (RT-PCR) also revealed that OCT4, KLF4, Nanog, and Sox2 levels increased compared to controls by 4.01-, 3.51- and 1.26-, and 2.5-fold, respectively, in A549 cancer cells. In Colo-205 cells, OCT4, KLF4, and Sox2 were increased by 1.79-, 13.17-, and 2.25-fold, respectively. Metadichol treatment with triple-negative primary breast cancer (HCAF-TNPBC) primary cancer cells led to multifold increases in OKSM factors by 19-, 6-, 8.07-, 2.45-, and 6.91-fold in concentration ranges of 1 picogram to 100 nanograms. Metadichol is a natural product that induces the expression of Yamanaka factors needed for reprogramming and Klotho, an antiaging gene, and curbs the expression of the TP53 gene, which is critical for reprogramming somatic cells into IPSCs. Metadichol increases endogenous vitamin C levels, leading to the efficient reprogramming of somatic cells into iPSCs. Metadichol is nontoxic and commercially available as a nutritional supplement. Thus, it can be directly tested in vivo in human subjects to confirm that cells can indeed be programmed into a state of induced pluripotency and cause the mitigation of disease conditions.
Genome-wide identification, characterization, and expression patterns of the PHD-Finger Family Genes in Wheat (Triticum aestivum L.): Molecular evolution and expression profiles during different stresses
Authors: Hao Xiangyang , Pang Fei , Fu Yihan , Liu Zhaoliang , Niu Junqi , Huang Wei , Wang Zhen 
Published: June 2022

Abstract
The Plant homeodomain (PHD) transcription factors (TFs) are a class of proteins with conserved Cys4-His-Cys3 domains that play important roles in plant growth and development and in response to abiotic stresses. Although characterization of PHDs have been performed in plants, little is known about their function in wheat (Triticum aestivum), especially under stress conditions. This study, 244 TaPHDs were identified in wheat using the comparative genomics method. we renamed them TaPHD1-244 on their chromosomal distribution and almost all PHD proteins are located in the nucleus. According to the un-rooted neighbor joining phylogenetic tree, gene structure and motif analyses, the PHD genes were divided into 4 clades. A total of 149 TaPHD gene pairs were assigned to arise from duplicate events. Furthermore, 230 gene pairs come from itself, 119, 186, 168, 7, 2 and 6 gene pairs from T. aestivum and six other species (H. vulgareto, Z. mays, O. sativa, A. thaliana, B.rapa and G. raimondii). 548 interacting protein branches were identified as involve in the protein interaction network. Tissue-specific expression pattern analysis showed that we found that TaPHDs were highly expressed in the stigma and ovary during flowering, suggesting that the TaPHD gene plays an active role in the reproductive growth of wheat. In addition, qRT-PCR results further confirmed that these TaPHD genes were involved in wheat abiotic stress response. In conclusion, our study provides a theoretical basis for deciphering the molecular functions of TaPHDs, especially in response to abiotic stress.
Comprehensive Mastocytosis Data Analysis From A Single Center
Authors: Tiryaki Tarık Onur , Özkan Sıdıka Gülkan , Erdem Simge , Aday Aynur Dağlar , Hindilerden İpek Yönal , Gelincik Aslı , Baykal Can , Yegen Gülçin , Doğan İbrahim Öner , Büyükbabani Nesimi , Nalçacı Meliha , Yavuz Akif Selim 
Published: June 2022

Abstract
Mastocytosis is a rare hematologic neoplasm that can present with involvement of different organs and variable complaints. The World Health Organization classification divides the disease into three prognostically distinct variants: Cutaneous mastocytosis (CM), systemic mastocytosis (SM) and mast cell sarcoma or localized mast cell (MC) tumors. SM is divided into subtypes according to the differences in organ involvement and signs of organ damage. The wide range of complaints may cause patients to consult various clinics, with resulting mis- or underdiagnosis. Therefore, the cooperation between different subspecialties is of paramount importance. In this article, we have compiled 104 adult mastocytosis cases diagnosed and followed in our Hematology clinic. The clinical and laboratory characteristics of the patients, therapies, complications and survival analysis is presented.
A climate-smart spatial planning framework
Authors: Buenafe Kristine Camille V. , Dunn Daniel C. , Everett Jason D. , Brito-Morales Isaac , Schoeman David S. , Hanson Jeffrey O. , Dabalà Alvise , Neubert Sandra , Cannicci Stefano , Kaschner Kristin , Richardson Anthony J. 
Published: June 2022

Abstract
1. Climate change is already having profound effects on biodiversity, but climate change adaptation has yet to be fully incorporated into area-based management tools used to conserve biodiversity, such as protected areas. One main obstacle to its inclusion is the lack of consensus regarding how impacts of climate change can be included in spatial conservation plans.2. We propose a climate-smart framework that prioritizes the protection of climate refugia—areas of low climate exposure and high biodiversity retention—identified using climate metrics. We explore four aspects of climate-smart spatial planning in the proposed framework: i) climate model ensembles; ii) multiple emission scenarios; iii) climate metrics; and iv) approaches to identifying climate refugia. We illustrate this framework in the Western Pacific Ocean, but it is equally applicable to terrestrial systems.3. All aspects of climate-smart spatial planning considered affected the configuration of spatial plans. The choice of climate metrics and approaches to identifying refugia result in large differences in climate-smart spatial plans, whereas the choice of climate models and emission scenarios have smaller effects. As configuration of spatial plans depended on climate metrics used, a spatial plan based on a single measure of climate change (e.g., warming) will not necessarily be robust against other measures of climate change (e.g., ocean acidification). We recommend including climate metrics most relevant for the biodiversity considered. To include the uncertainty associated with different climate futures, we recommend using multiple climate models (i.e., an ensemble) and emission scenarios. Finally, we show that the approaches we used to identify climate refugia come with trade-offs between the degree to which they are climate-smart and their efficiency in meeting conservation targets. Hence, the choice of approach will depend on the relative value stakeholders place on climate change adaptation.4. By using this framework, protected areas can be designed with improved longevity and thus safeguard biodiversity against current and future climate change. We hope that the proposed climate-smart framework helps transition conservation planning towards climate-smart approaches.
Comparing Eurocode 8-5 and AASHTO methods for earth pressure analysis against centrifuge tests, finite elements, and the Generalized Coefficients of Earth Pressure
Authors: Pantelidis Lysandros , Christodoulou Panagiotis 
Published: June 2022

Abstract
This paper presents an exhaustive comparison of the earth pressure methods included in EN1998-5:2004 (use of Mononobe-Okabe method, M-O), prEN1998-5:2021 and AASHTO (M-O with half peak ground acceleration) standards, against contemporary centrifuge tests, finite elements, and the method proposed by the first author in 2019. The latter is a continuum mechanics approach for deriving earth pressure coefficients for any soil state between the “at-rest” state and the active or passive state, applicable to cohesive-frictional soils and both horizontal and vertical pseudo-static conditions. The same method also provides analytical expressions for the calculation of the required wall movement for the mobilization of the active (or passive) failure state, as well as for the mobilized shear strength values of soil. The comparison includes among others, centrifuge test results from two different studies, and results from 157 finite element models from two different programs. All experimental, numerical, and analytical results show that the EN19985-5:2004 method gives conservative to excessively conservative active earth pressure values, AASHTO’s approach fairly good behavior, while prEN1998-5:2021 excessively conservative values. For the passive state, all methods included in the above-mentioned standards, return unreliable and unconservative results. In contrast, the results obtained by the proposed method show a remarkable agreement with the respective ones obtained both by the finite element method and the various centrifuge tests. Also, it is shown that a pseudo-static earth retention analysis, can be replaced by a respective static analysis, just substituting the (c', φ') values of soil of the original problem with the mobilized ones.
WITHDRAWN: Decellularized lung extracellular matrix scaffold promotes human embryonic stem cell differentiation towards alveolar progenitors
Authors: Noori Afshin , Dezfouli Mohammad Reza Mokhber , Rajabi Sarah , Ganji Fatemeh , Ghezelayagh Zahra , Agha Elie El , Baharvand Hossein , Chaleshtori Sirous Sadeghian , Tahamtani Yaser 
Published: June 2022

Abstract
Background: Efficient production of alveolar epithelial cells and achieving optimal functionality and maturity has presented a serious challenge in recent years. The extracellular matrix (ECM) provides a dynamic environment and mediates cellular responses during both development and tissue repair. Decellularized ECM (dECM) which retains its native-like structure and biochemical composition provides the signals needed to induce differentiation into tissue-specific lineages in vitro. Results: Here, we repopulated lung ECM-derived scaffold (scaffold) with human embryonic stem cells (hESCs) derived lung progenitor cells and compared with the cells grown on either lung ECM-derived hydrogel (hydrogel) or fibronectin-coated plates in 2D cultures. We found that scaffold preserved its composition and native structures. All groups displayed progenitor cell differentiation as revealed by the expression of NKX2.1, P63, and CK5. Although no significant differences in cell viability between groups, cells differentiated on scaffold and hydrogel showed significant upregulation of SOX9 (a marker of the distal airway epithelium), cells differentiated on scaffold showed enhanced expression of SFTPC (AT2 marker), FOXJ1 (ciliated cell marker) and MUC5A (secretory cell marker). Conclusion: Overall, our results suggest that scaffold improves differentiation of progenitors into alveolar type 2 cells in comparison with hydrogel and fibronectin as well as efficient retention and homing to the alveolar region.
Effect of pollution load reduction on water quality in rural lakes in the shallow hill water network area
Authors: Li Jingjing , Chen Yingshu , Wang Zhe 
Published: June 2022

Abstract
There is poor water environment quality in rural lakes in the shallow hill water network area north of the Han River on the Chinese Jianghan Plain due to their poor hydrodynamic conditions. We herein selected ten typical rural lakes to simulate water environment improvement. The hydrodynamic and water quality models were built based on MIKE21, while the simulation compared the year-round trends in lake water quality in the current and planning year, and demonstrated the possibility of recharging rural lakes from the backbone of the Han River–Three Inland Rivers networks. The results show that the water quality of the ten lakes has improved significantly after pollution control, with an improvement rate of more than 70%. Pollution interception and management are particularly successful for improving lake water quality in rural lakes. The replenishment of water from the backbone river to the lakes could enhance the fluidity of the rural lakes but cannot eliminate the total phosphorus (TP) risk. Therefore, in the relatively closed water bodies of rural lakes in the shallow hilly water network area, pollution intercept and control is key to controlling TP, and taking measures such as increasing lake hydrodynamic conditions in summer will be a more efficient approach to improve rural lake water quality.