bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immediate versus Postponed Drainage for Infected Necrotizing Pancreatitis: a protocol for a systematic review and meta-analysis
Authors: Hu Yu-wei , Zeng Qin , Yang Guang-yu , Wang Yu , Wang Lu , Sun Ming-wei , Zeng Jun , Jiang Hua , Wang Kai 
Published: July 2022

Abstract
BACKGROUND
Acute pancreatitis is the most common gastrointestinal disease worldwide for hospital admission, and infected pancreatic and peripancreatic necrosis is a potentially lethal complication, which is the most dreadful one associated with a poor prognosis: mortality is approximately 15% and up to 30–39%. Depending on the very high mortality and morbidity, infected necrotizing pancreatitis always requires intervention at the early stage. The current standard approach for infected necrotizing pancreatitis is a minimally invasive step-up approach with catheter drainage as the first step. International guidelines advise delay in catheter drainage and antibiotics treatment until the infected collection has become a “wall-off”, which takes 4 weeks to develop. However, no clear evidence from clinical studies was seen to suggest superiority for the postponed catheter drainage. We will perform the first systematic review and meta-analysis to explore whether immediate catheter drainage improves the survival rate of patients with infected necrotizing pancreatitis compare with postponed catheter drainage.
Methods
We will search the randomized controlled trial (RCT) literature involving and nonrandomized clinical cohort studies of immediate catheter drainage in patients with infected necrotizing pancreatitis in 5 electric databases, including PubMed, Web of Science, the Cochrane Library, Chinese National Knowledge Infrastructure (CNKI) and Chinese Biomedical Literature Database (CBM). Studies including patients under 18 years of age or pregnant women will be excluded. We will define the overall mortality of patients with infected necrotizing pancreatitis as the primary outcome. Besides, the incidences of bleeding resulting in intervention, perforation of a visceral organ leading to intervention, enterocutaneous fistula, pancreatic cutaneous fistula, incisional hernia and wound infection, the total length of stay, endocrine and exocrine pancreatic insufficiency, and severe complications will be regarded as the secondary outcomes. Quality assessment of the included studies will be independently performed according to the Version 2 of the Cochrane tool for assessing the risk of bias in a randomized trial (RoB2) for RCTs and the Non-Randomized Studies-of Interventions (ROBINS-I) Tool for Observational Studies. Meanwhile, the level of evidence for results will be assessed by using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) method. All analyses will be conducted by using the RevMan (Version 5.4; Cochrane, Oxford, UK).
Results
From our study, we will ascertain whether immediate catheter drainage improves the survival rate of patients with infected necrotizing pancreatitis compare with postponed catheter drainage.
Trial registration:
PROSPERO registration number CRD42021291734.
Synergistic antitumor effect of all-trans retinoic acid on regorafenib-treated human colon cancer cell lines via modulation of the AMPK/VEGF/ERK/NFKB/caspase-3 signaling axis
Authors: Hamada marium , Mehanna Radwa , Hasseb Mohammad , Houssen Maha 
Published: July 2022

Abstract
Purpose
Colon cancer is the second most common cause of cancer-related death worldwide. Angiogenesis mediated by vascular endothelial growth factor (VEGF) plays important roles in cancer progression, and activated mitogen protein kinase (AMPK) has also been shown to exert both positive and negative effects on angiogenesis and different oncogenic signaling pathways. The multikinase inhibitor regorafenib prevents the activation of numerous kinases involved in angiogenesis, proliferation, and metastasis. All-trans retinoic acid (ATRA) has also been demonstrated to inhibit the growth and the development of different types of tumors. This study aims to assess the antitumor effects of ATRA in regorafenib-treated human colon cancer cell lines and investigate the molecular mechanism mediating these effects.
Methods
The levels of VEGF, AMPK, extracellular signalregulated protein kinase 1 (ERK1), and nuclear factor kappa B (NF-κB) were analyzed using enzyme-linked immunosorbent assay, and caspase-3 activity was assessed colorimetrically.
Results
ATRA potentiated AMPK and caspase-3 activities and reduced the levels of VEGF, ERK1, and NF-κB levels in regorafenib-treated human colon cancer cell lines.
Conclusion
ATRA exerted synergistic antiproliferative, antiangiogenic, and proapoptotic effects on regorafenib-treated colorectal cancer cells by modulating the AMPK/VEFG/ERK/NF-κB/caspase-3 signaling axis.
Potential molecular mechanisms of postoperative restenosis in lower extremity atherosclerotic occlusive disease
Authors: lu zilin , Jian Dong , Li Shuangshuang , Ma Chao , Lu Feng , Li Mingtao , Du Pengcheng , Zhou Jian 
Published: July 2022

Abstract
Arteriosclerosis obliterans of lower limbs is a common peripheral arterial disease in clinics. With the development of endovascular therapy, the commonly used clinical treatment methods include balloon stent endovascular therapy and plaque resection. However, these treatment methods are often accompanied by high restenosis rate. This study aims to find a new treatment method from the genetic level.
Association between School Mask Mandates and SARS-CoV-2 Student Infections: Evidence from a Natural Experiment of Neighboring K-12 Districts in North Dakota
Authors: Sood Neeraj , Heick Shannon , Stevenson Josh , Høeg Tracy 
Published: July 2022

Abstract
There is still considerable debate about whether mask mandates in the K-12 schools limit transmission of SARS-CoV-2 in children attending school. Randomized data about the effectiveness of mask mandates in children is still entirely lacking. Our study took advantage of a unique natural experiment of two adjacent K-12 school districts in Fargo, North Dakota, one which had a mask mandate and one which did not in the fall of the 2021-2022 academic year. In the winter, both districts adopted a masks-optional policy allowing for a partial crossover study design. We observed no significant difference between student case rates while the districts had differing masking policies (IRR 0.99; 95% CI: 0.92 to 1.07) nor while they had the same mask policies (IRR 1.04; 95% CI: 0.92 to 1.16). The IRRs across the two periods were also not significantly different (p = 0.40). Our findings contribute to a growing body of literature which suggests school-based mask mandates have limited to no impact on the case rates of COVID-19 among K-12 students.
Double narrowband induced perfect absorption photonic sensor based on graphene-dielectric-gold hybrid metamaterial
Authors: Liu Zhimin M. , Zhuo Shanshan S. , Zhou Fengqi Q. , Zhang Xiao , Qin Yipeng P. , Luo Xin , Ji Cheng , Yang Guangxin X. 
Published: July 2022

Abstract
Double narrowband induced perfect absorption in the terahertz region is achieved in a graphene-dielectric-gold hybrid metamaterial, whose physical mechanism is analyzed using the coupled-mode theory (CMT), which agreed well with the finite-difference time-domain (FDTD) simulation. This study found that the Fermi level of graphene can be adjusted to improve the absorptivity when the refractive index (RI) nd of the chosen dielectric cannot achieve a good absorption effect. In addition, the blue shift of absorption spectrum can be used in the design of dual-frequency electro-optical switches, of which the modulation degree of amplitude (MDA) can reach as high as 94.05% and 93.41%. Most significantly, the RI sensing performance is investigated, which shows an ultra-high absorption sensitivity SA = 4.4 o/RIU, wavelength sensitivity Sλ = 9.8 o/RIU, and phase shift sensitivity Sφ = 2691 o/RIU. At last, an interesting finding is that the two peaks (R1 and R2) of plasmon-induced absorption (PIA) show different polarization characteristics (insensitive or sensitive) to the incident light angle. Undoubtedly, this paper is of great significance to the research and design of terahertz photonic devices and sensors.
CSF1R Contributes to Vascular Association of Microglia and Stress Regulation in Schizophrenia
Authors: Tian Li , Yan Ling , Li Yanli , Fan Fengmei , Xuan Fang-Ling , Feng Wei , Chithanathan Keerthana , Li Wei , Huang Junchao , Li Hongna , Gou Mengzhuang , Chen Wenjin , Tian Baopeng , Wan Zhiren , Tan Shuping , Zharkovsky Alexander , Hong Elliot , Tan Yunlong 
Published: July 2022

Abstract
Whether and how microglia contribute to stress and anxiety in schizophrenia are not well established. We hypothesized that microglial colony stimulating factor 1 receptor (CSF1R) regulates stress susceptibility in schizophrenia via the hippocampus and associated cortical regions. A cohort of first-episode schizophrenia (FES) patients (n=51) and age- and sex-paired healthy controls (HCs) (n=46) were first recruited. Compared to HCs, FES patients showed higher scores of perceived stress scale (PSS, p<0.01), lower levels of CSF1R mRNA (Log2FC=-0.195, FDR=0.003) and protein (18.31±0.95 vs. 20.49±0.90 µg/ml, p<0.05) in the blood, and smaller volumes of the superiorfrontal gyrus (39.13±0.64 vs. 40.80±0.76 cm3, FDR<0.05) and parahippocampal gyrus (3.51±0.048 vs. 3.72±0.058 cm3, FDR<0.05). CSF1R facilitated the negative superiorfrontal gyral association with PSS (β=6.109, 95% CI: 1.642~10.578, p<0.01) in HCs but not FES patients. CSF1R-associated gene network contributed to brain development. We further studied a chronic unpredictable stress (CUS) mouse model combined with a CSF1R inhibitor (CSF1Ri). Both CUS and CSF1Ri enhanced anxiety in mice (both p<0.001). RNA-seq revealed downregulation of genes for cell adhesion and angiogenesis after CUS-CSF1Ri treatment. Immunostaining showed downregulation of CD31 and preferential loss of vessel-associated IBA1+-microglia induced by CUS or CSF1Ri (p<0.05). Oligodendrocyte precursor cells were however increased after CUS-CSF1Ri treatment (p<0.01). These results suggest that microglial CSF1R showed a protective effect on stress and anxiety in FES patients and CUS mice via contribution to angiogenesis.
Blockade of αvβ8 integrin synergizes with checkpoint inhibition to enhance anti-tumor immunity through Cxcl9-dependent augmentation of CD8+ T cell cytotoxicity
Authors: Larrick Jasmine , Ma Hsiao-Yen , Whitty Caroline , Luster Andrew , Ren Xin , Sheppard Dean 
Published: July 2022

Abstract
Inhibition of the TGF-β activating integrin, αvβ8, can dramatically enhance the effectiveness of checkpoint inhibition in multiple checkpoint resistant tumor models, but the mechanisms underlying this dramatic synergy have not been previously explored. In this study, we used single cell RNA sequencing to investigate the mechanisms of synergy between inhibition of αvβ8 and PD1 in the EMT-6 model, a checkpoint resistant model of triple negative breast cancer. Combination therapy dramatically enhanced interferon gamma (Ifnγ) expression by CD8+ and CD4+ T cells, activated interferon signaling throughout the tumor microenvironment, specifically increased Cxcl9 secretion by tumor macrophages and enhanced Gzmb expression and tumor cell killing by CD8+ T cells. The protective effects of combination therapy were inhibited by Cxcl9 blockade. These findings suggest that Ifnγ-mediated induction of Cxcl9 is an important driver of synergy between αvβ8 and checkpoint inhibition and raise the possibility that Cxcl9 might be useful as an early PD biomarker of effective combination therapy.
A Novel Correlation Gaussian Process Regression-Based Extreme Learning Machine
Authors: HE Yulin , LI Xu , YUAN Zhenhao , Fournier-Viger Philippe , HUANG Joshua Zhexue 
Published: July 2022

Abstract
One obvious defect of Extreme Learning Machine (ELM) is that the prediction performance of ELM is sensitive to the random initialization of input-layer weights and hidden-layer biases. GPRELM integrating Gaussian Process Regression (GPR) into ELM is a newly-proposed, simple and effective strategy to make ELM insensitive to the random initialization. However, the kernel based GPRELM (kGPRELM) exists serious over-fitting. In this paper, we analyze the reason for over-fitting of kGPRELM in theory and further propose a correlation based GPRELM (cGPRELM) which uses the correlation coefficient to measure the similarity between two different hidden-layer output vectors. cGPRELM reduces the possibility that covariance matrix becomes an identity matrix with the increase of hidden-layer nodes and thus controls the over-fitting effectively. Meanwhile, cGPRELM works well for improper initialization intervals where ELM and kGPRELM can't provide the good predictions. The experimental results on real classification and regression data sets demonstrate the feasibility and superiority of cGPRELM, which not only obtains the better generalization performance but also has the lower computational complexity.
Characteristics of dentists and patients associated with appropriate antibiotic prescriptions by French dentists: a cross-sectional study using Health Insurance databases
Authors: Simon Maïa , Pereira Ouarda , Constant Marie-Louise , Guillet-Thibault Julie , Pulcini Céline , Thilly Nathalie 
Published: July 2022

Abstract
Background – The use of antibiotics in dental care is often unnecessary or inappropriate. Our objectives were to identify (i) clusters of dentists grouped according to their appropriateness score based on proxy indicators’ results; and (ii) dentists’ and patients’ characteristics associated with the appropriateness of antibiotic prescriptions.Methods – We used data of the Health Insurance reimbursement databases on antibiotics prescribed in 2019 by general dental practitioners of the Grand Est region in France. The appropriateness of antibiotic prescriptions was estimated by the results of recently published proxy indicators. We conducted a cluster analysis according to an appropriateness score calculated for each dentist, using the Ward method. We then conducted bivariate and multivariable analyses to identify characteristics associated with these clusters.Results – We included 3,014 dentists, which were grouped into three clusters. We identified several characteristics significantly associated with the appropriateness of antibiotic prescriptions. Overall, dentists had more appropriate prescription practices when they were male, having a predominant surgery practice in the Lorraine area for less years, when they had healthier patients (i.e. younger, with no chronic diseases, and who received less procedures), and when they had a more prudent use of drugs in general (i.e. less drug expenses, less prescriptions of all drugs, less prescriptions of antibiotics and less prescriptions of systemic non-steroidal anti-inflammatory drugs).Conclusions – We identified clusters and characteristics associated with the appropriateness of antibiotic prescriptions made by dentists, which might help guiding antimicrobial stewardship interventions.
Comparison of the upper and lower airway microbiome in early postoperative lung transplant recipients
Authors: Li Chun-xi , You Zhi-xuan , Liu Hai-yue , Lin Yan-xia , Pan Jian-bing , You Chang-xuan , Su Jin 
Published: July 2022

Abstract

Background
The unique airway microbiome is associated with the development of transplant complications. It is necessary to explore an appropriate sampling strategy because of the irrationality and infeasibility for long-term collection of lower airway samples for microbial analysis. Using 16S rRNA gene sequencing, we compared paired nasal swab (NS) and bronchoalveolar lavage fluid (BALF) microbiome from 17 early postoperative lung transplant recipients (LTRs).
Results
The microbiome between the two compartments were significantly different in Shannon diversity and beta diversity, but similar in richness (observed OTUs). Planococcaceae sp., Corynebacterium, Staphylococcus and Stenotrophomonas were identified as core NS-associated microbiome. Xanthomonadaceae sp., Ralstonia, Stenotrophomonas, Pedobacter, Chryseobacterium, Planococcaceae sp., Variovorax and Comamonadaceae sp. were identified as BALF‐associated microbiome. In addition, other differences were observed between the two samples. The within-subject dissimilarity was higher than the between-subject dissimilarity, indicating a greater impact of sampling sites than sampling individuals on microbial difference.
Conclusions
There were both difference and homogeneity between NS and BALF microbiome in early postoperative LTRs. High levels of pathogens were detected in both samples, suggesting that both of them can reflect the diseases characteristics of transplanted lung. The differences between upper and lower airway microbiome mainly come from sampling sites instead of sampling individuals.
Immuno-enhancement effects of barely β-glucan and barley extracts in arsenic trioxide -induced immunosuppressed rat model
Authors: El-Rahman Hanaa Abd 
Published: July 2022

Abstract
Barely β-glucans are polysaccharides that are biologically active and have antioxidant, immunomodulatory, and anti-inflammatory activities. This study examined the effects of barely β-glucan and barley extracts on sodium trioxide-induced immunosuppression. Fifty-four male albino rats (weighing 100 ± 25 g) were divided into eight groups, each including six rats in this investigation. Group 1: PBS was injected intraperitoneally into the rats, while saline was given orally (negative control). Groups 2–8 received daily intraperitoneal injections of As2O3 (5 mg/kg) for 4 weeks. The second group serves as a positive control. The rats in groups 3, 4, and 5 were given daily doses of β-glucan extracts of 40, 80, and 160 mg/kg bw/day, respectively, with ATO (arsenic triodide). Groups 6, 7, and 8 were given barley extract at doses of 0.7,1.4, and 2.8 g/kg bw/day, respectively, with ATO. After the treatments were completed, all of the rats were slaughtered for biochemical, hematological, and histopathological analysis. Barley β-glucan and barley extracts increased CAT and GPx activity while lowering MDA levels in the spleen tissue of rats. They also improved haematological and histopathological changes caused by arsenic trioxide. The levels of IgG and IgM in the groups treated with barley β-glucan extract were considerably greater than those in the groups treated with barley extract. The cytokine levels, hepatic, and renal biomarker markers were all affected by arsenic trioxide. Our findings show that barley extracts and barely β-glucan extracts could be employed as a component of functional foods to boost immunity.
Microbiome-inspired phyllospheric synthetic communities increase microbial diversity and productivity of field-grown plants of Agave tequilana
Authors: Flores-Núñez Víctor , Camarena-Pozos David , Chávez-González Daniel , Alcalde-Vázquez Raúl , Vázquez-Sánchez Nélida , Hernández-Melgar Alan , Xool-Tamayo Jorge , Moreno-Ulloa Aldo , Partida-Martínez Laila 
Published: July 2022

Abstract
Agaves are plants native to North America that sustain life in arid and semi-arid ecosystems.
Previous studies revealed that cultivated plants of Agave tequilana had lower microbial diversity and functionality compared to wild species. Here, we tested if microbiome-inspired phyllospheric synthetic communities, based on microbial hubs or enriched phyllospheric microbial functions, could increase microbial diversity and plant fitness in A. tequilana.
We applied seven functional and three random syncoms on one-year-old plants of A. tequilana in the field and monitored their development for two years. Phylogenetic analyses revealed that the composition and diversity of the phyllospheric prokaryotic and eukaryotic communities associated with A. tequilana were affected by the season, with greatest diversity observed during the dry months. The addition of functional syncoms promoted phyllospheric microbial communities with a higher diversity, greater capacity to consume carbon sources, and more integrated and complex networks. Additionally, plants treated with functional syncoms had an increased content of sugars (oBrix, a measure of productivity in agaves) in the stems, and higher leaf metabolic diversity.
Our work demonstrates that inoculation of syncoms formulated based on functional analyses of microbiome data represents an effective biotechnological tool to improve the sustainability and productivity of crops of arid ecosystems.
Impacts of residual 3D printing metal powders on immunological response and bone regeneration: An in vivo study
Authors: Tang Jincheng , Sang Zhuo , Zhang Xiaolei , Song Changhui , Tang Wei , Luo Xiaoping , Yan Ming 
Published: July 2022

Abstract
Background
Residual powder is a defect in powder bed fusion-based additive manufacturing (3D printing), and it is difficult to completely remove it from as-printed materials. In addition, it is not necessary to apply 3D printed implants with residual powder in the clinic. The immunological response triggered by the residual powder is an important area of study in medical research.
Methods
To further understand the possible immunological reactions and hidden dangers caused by residual powders in vivo, this study compared the immunological reactions and osteolysis caused by typical powders for four implant materials: 316L stainless steel, CoCrMo, CP-Ti, and Ti-6Al-4V (particle size range of 15–45 µm), in a mouse skull model. Furthermore, the possible immunological responses and bone regeneration induced by the four 3D printed implants with residual powder in a rat femur model were compared.
Results
In the mouse skull model, it was found that the 316L-S, CoCrMo-S, and especially the 316L-M powders, upregulated the expression of pro-inflammatory factors, increased the ratio of RANKL/OPG, and activated more functional osteoclasts, resulting in more severe bone resorption compared with those in other groups. In the rat femur model, which is more suitable for clinical practice, there is no bone absorption in implants with residual powders, but they show good bone regeneration and integration ability because of their original roughness. The results indicate that the expressions of inflammatory cytokines in all experimental groups were the same as those in the control group, showing good biological safety.
Conclusions
The results answered some critical questions related to additively manufactured medical materials in vivo and indicated that as-printed implants may have great potential in future clinical applications.
Circular RNA hsa_circ_0117530 sequesters miR-874 to upregulate ZEB1 and promote cervical carcinoma progression
Authors: Wang Yijun , Chen Aner , Zheng Chenyang , Zhao Lingjun 
Published: July 2022

Abstract
Cervical cancer is one of the three most common tumors in women. In this study, we found that ZEB2 was significantly overexpressed in cervical cancer. It was found that that circular RNA circ-0117530 transcribed by ZEB2 was significantly overexpressed in cervical cancer tissues. Circ-0117530 promoted growth, invasion, and migration in cervical cancer cell lines. Dual-luciferase assays showed that that circ-0117530 regulated ZEB1 expression by sponging miR-874. These results suggest that the ZEB family is closely related to the occurrence and development of cervical cancer. Overall, we found that circ-0117530 regulates the occurrence and development of cervical cancer through the miR-874/ZEB1 axis, which may provide a potential new therapeutic target for the treatment of cervical cancer.