bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep Learning Identified Extra-Prostatic Extension and Seminal Vesicle Invasion as an MRI Biomarker for Prostate Cancer Outcomes
Authors: Hossain Sajid , Hossain Saeed , Sritharan Durga , Fu Daniel , Nene Aishwarya , Hossain Jahid , Chadha Saahil , Kim Issac , Lin MingDe , Aboian Mariam , Aneja Sanjay 
Published: January 2025

AbstractCurrent risk stratification methods for localized prostate cancer (PCa) are reliant on clinical and pathological variables that do not easily account for location of cancer spread. Prostate MRI is a helpful tool to identify anatomic extra-prostatic cancer spread (EPE) and seminal vesicle invasion (SVI) but is subject to radiologist expertise and inter-observer variation. We report deep learning models which provide objective end-to-end evaluation of EPE and SVI on prostate MRI. Both EPE and SVI models demonstrate high discriminatory ability on three held-out test sets spanning different clinical settings, equipment manufacturers, and MRI magnet strengths. Interpretability studies suggest both EPE and SVI models identify clinically-relevant anatomic regions. Lastly, we show that classification of EPE and SVI by our models is independently associated with increased risk of biochemical recurrence (BCR) following localized treatment. Furthermore, we demonstrate that our models can be easily integrated to well-established risk stratification methods (NCCN and UCSF-CAPRA) for improved ability to identify high risk PCa phenotypes.
Detection of Hemagglutinin H5 influenza A virus RNA and model of potential inputs in an urban California sewershed
Authors: Paulos Abigail P. , Hilton Stephen P. , Shelden Bridgette , Duong Dorothea , Boehm Alexandria B. , Wolfe Marlene K. 
Published: January 2025

AbstractIn 2024, the highly pathogenic avian influenza A H5N1 caused outbreaks in wild birds, poultry, cows, and other mammals in the United States with 61 human cases also reported by the CDC. Detection of influenza A H5 RNA in wastewater has been previously reported in sewersheds in Texas and North Carolina with nearby impacted dairy herds following the emergence of H5N1 in dairy cows. Here, we conduct retrospective testing of total influenza A and H5 hemagglutinin genes in wastewater as well presenting and applying new assays for detection of H1 and H3 genes across a respiratory virus season in an urban California sewershed from September 2023 – May 2024. Total influenza A, H1, and H3 were regularly detected, while H5 was first detected in March. We developed a model that uses Monte Carlo simulations and previously published parameters to estimate numbers of infected people, poultry, wild birds, or liters of H5-contaminated milk required to result in measured H5 concentrations in wastewater. Our findings demonstrate that in this California sewershed, contaminated milk or infected poultry were the most likely sources of H5 to wastewater. We created a publicly available tool to apply the H5 input model in other sewersheds estimate required inputs.SynopsisWe developed a model to understand potential sources of influenza A H5 RNA in wastewater, enabling interpretation of H5 RNA wastewater detections.TOC figure
The limit of life at extremely low water activity: Lithium-concentration ponds in a solar saltern (Salar de Atacama, Chile)
Authors: Demergasso C. , Avendaño P , Escuti C. , Veliz R. , Chong G. , Pedrós-Alió C. 
Published: January 2025

ABSTRACTExtremely hyper-saline ponds from an industrial lithium-concentration process in solar salterns in the Atacama Desert were studied to determine the limits of life at very low water activity. Water activity (aw) of 0.61 is the lowest awvalue for growth of living beings recorded to date. Xerophilic (sometimes called osmophilic) filamentous fungi and yeasts are predominant in high-sugar foods with such low awvalues. Some microorganisms are capable of growth at that water activity level. By contrast, high-salt environments are almost exclusively populated by prokaryotes, notably theHalobacteriaclass and someBacteroidetes, capable of growing in saturated NaCl (aw0.75). The lowest awthat can be achieved by the addition of NaCl is 0.75 (saturation point for NaCl). Crystallizer ponds in Li+concentration plants reach down to water activity levels around 0.1. The aim of this study was to determine how far along the salinity gradient could life be found. Cell counts were attempted by epifluorescence microscopy and qPCR with bacterial and archaeal universal primers. Biomass for DNA extraction was obtained by an optimized protocol involving dialysis of brines previously fixed with ethanol. Prokaryotic diversity was studied by DNA extraction, PCR, qPCR and 16S rRNA amplicon sequencing in different ponds along the salinity gradient. Archaeal DNA was found in the lower salinity ponds, while bacterial DNA was found along the whole gradient. Bacterial cDNA was retrieved from ponds down to an awof 0.2. Moreover, bacteria could be grown in enriched cultures from most ponds.
Acquisition of discrete immune suppressive barriers contributes to the initiation and progression of preinvasive to invasive human lung cancer
Authors: Yoffe Liron , Bhinder Bhavneet , Kang Sung Wook , Zhang Haoran , Singh Arshdeep , Ravichandran Hiranmayi , Markowitz Geoffrey , Martin Mitchell , Kim Junbum , Zhang Chen , Elemento Olivier , Tansey Wesley , Bates Stewart , McGraw Timothy E. , Borczuk Alain , Lee Hyun-Sung , Altorki Nasser K. , Mittal Vivek 
Published: January 2025

AbstractComputerized chest tomography (CT)-guided screening in populations at risk for lung cancer has increased the detection of preinvasive subsolid nodules, which progress to solid invasive adenocarcinoma. Despite the clinical significance, there is a lack of effective therapies for intercepting the progression of preinvasive to invasive adenocarcinoma. To uncover determinants of early disease emergence and progression, we used integrated single-cell approaches, including scRNA-seq, multiplexed imaging mass cytometry and spatial transcriptomics, to construct the first high-resolution map of the composition, lineage/functional states, developmental trajectories and multicellular crosstalk networks from microdissected non-solid (preinvasive) and solid compartments (invasive) of individual part-solid nodules. We found that early disease initiation and subsequent progression are associated with the evolution of immune-suppressive cellular phenotypes characterized by decreased cytotoxic CD8 T and NK cells, increased T cell exhaustion and accumulation of immunosuppressive regulatory T cells (Tregs) and M2-like macrophages expressing TREM2. Within Tregs, we identified a unique population of 4-1BB+ Treg subset enriched for the IL2-STAT5 suppressive pathway with transcription profiles supporting discrete metabolic alterations. Spatial analysis showed increased density of suppressive immune cells around tumor cells, increased exhaustion phenotype of both CD4 and CD8 T cells expressing chemokine CXCL13, and spatial micro-complex of endothelial and lymphocyte interactions within tertiary lymphoid structures. The single-cell architecture identifies determinants of early disease emergence and progression, which may be developed not only as diagnostic/prognostic biomarkers but also as targets for disease interception. Additionally, our dataset constitutes a valuable resource for the preinvasive lung cancer research community.
CD44 and Ezrin restrict EGF receptor mobility to generate a novel spatial arrangement of cytoskeletal signaling modules driving bleb-based migration
Authors: Jha Ankita , Chandra Ankit , Farahani Payam , Toettcher Jared , Haugh Jason M. , Waterman Clare M. 
Published: January 2025

AbstractCells under high confinement form highly polarized hydrostatic pressure-driven, stable leader blebs that enable efficient migration in low adhesion, environments. Here we investigated the basis of the polarized bleb morphology of metastatic melanoma cells migrating in non-adhesive confinement. Using high-resolution time-lapse imaging and specific molecular perturbations, we found that EGF signaling via PI3K stabilizes and maintains a polarized leader bleb. Protein activity biosensors revealed a unique EGFR/PI3K activity gradient decreasing from rear-to-front, promoting PIP3 and Rac1-GTP accumulation at the bleb rear, with its antagonists PIP2 and RhoA-GTP concentrated at the bleb tip, opposite to the front-to-rear organization of these signaling modules in integrin-mediated mesenchymal migration. Optogenetic experiments showed that disrupting this gradient caused bleb retraction, underscoring the role of this signaling gradient in bleb stability. Mathematical modeling and experiments identified a mechanism where, as the bleb initiates, CD44 and ERM proteins restrict EGFR mobility in a membrane-apposed cortical actin meshwork in the bleb rear, establishing a rear-to-front EGFR-PI3K-Rac activity gradient. Thus, our study reveals the biophysical and molecular underpinnings of cell polarity in bleb-based migration of metastatic cells in non-adhesive confinement, and underscores how alternative spatial arrangements of migration signaling modules can mediate different migration modes according to the local microenvironment.
Evidence for distinct networks underlying symptom clusters of posttraumatic stress disorder
Authors: Hauner Katherina K. , Chau Aileen , Krueger Frank , Gordon Barry , Grafman Jordan 
Published: January 2025

AbstractBackgroundClinical and psychometric evidence has long supported a multidimensional model of PTSD, with symptom subcategories derived from factor analytic methods. Although research on the biological bases of PTSD as a unitary construct is profuse, comparatively few studies have examined the neural mechanisms underlying subcategories of PTSD symptoms. The present study aimed to provide the first evidence of causal relationships between brain structure and PTSD symptom subcategories, using a lesion-behavior mapping approach.MethodsUsing a group of male combat veterans with focal penetrating traumatic brain injuries (n= 177), we determined the effects of focal damage on the PTSD symptom subcategories of hyperarousal, avoidance, and re-experiencing.ResultsOur findings revealed two distinct networks that underlie symptom subcategories of PTSD: (1) an amygdala-ventromedial prefrontal cortex network underlying hyperarousal and avoidance symptoms, in which amygdala damage acts as a risk factor for the development of these symptoms, while vmPFC damage acts as a protective factor against the same symptoms; and (2) a hippocampal network underlying re-experiencing and avoidance, in which hippocampal damage acts as a protective factor against these symptoms.ConclusionsThe present study provides novel insights regarding the causal role of key brain regions in the heterogeneous expression of PTSD symptoms. Results not only contribute to a more complete picture of the neural mechanisms underlying PTSD, but may also aid in the future development of individualized therapeutic strategies that target specific symptom profiles.
Meaningful organizational routines in primary healthcare: influencing factors and their consequences for routine enactment
Authors: Jacobsson Mattias , Näsholm Malin 
Published: January 2025

PurposeBased on the well-known risks associated with deviating from established routines in primary healthcare and the positive consequences of upholding them, the purpose of this study is to increase the understanding of the role of meaningfulness in the enactment of organizational routines.Design/methodology/approachThe study is based on 24 semi-structured interviews with three different professional categories in primary healthcare in Sweden. The data were analyzed using thematic analysis on a latent level, combined with a two-factor model as sensitizing concepts.FindingsDifferences are identified between factors that reduce meaninglessness (called “sufficiency factors”) and those that enable meaningfulness (called “meaningfulness factors”). Nine sufficiency factors and six meaningfulness factors explain what makes organizational routines perceived as meaningful by the different professional groups. A two-factor matrix is developed that highlights the intricate challenges associated with routine enactment based on these factors.Originality/valueThe study is unique in that it is the first to integrate research on organizational routines and meaningfulness. However, understanding meaningful organizational routines is not only essential because it is an overlooked area in both of these two streams of research but also because of its clear, practical relevance in the primary healthcare setting.
P2Y2 Inhibition Modifies the Anabolic Response to Exercise in Adult Mice
Authors: Chougule Amit , Zhang Chunbin , Denbow Jordan , Vinokurov Nickolas , Mendez Devin , Vojtisek Elizabeth , Gardinier Joseph 
Published: January 2025

ABSTRACTAs the aging population continues to grow, the incidence of osteoporotic fractures increases and is compounded by our lack of therapeutic strategies that increase bone formation. Although exercise and physical activity play a key role in maintaining bone mass throughout our lives, the loads and exertion required to elicit an anabolic response becomes exceedingly difficult to achieve with age. Based on previous work, the P2Y2 receptor offers a unique therapeutic target to increasing bone mass by modifying the mechanotransduction. Others have also shown P2Y2 to have a negative effect on osteoblast function. However, the extent to which inhibiting P2Y2 pharmaceutically improves bone mass or the mechanotransduction of bone remains unknown. Our central hypothesis for this study states that inhibiting P2Y2 activity can enhance the anabolic response to loading in an aging population. To test this hypothesis, the anabolic response to exercise was examined by treating adult mice, which typically display a minimal response, with the P2Y2 inhibitor AR‐C118925XX (ARC). Our findings from this study demonstrate that ARC treatment of adult mice increases periosteal bone formation in response to exercise. The enhanced response to exercise was characterized by a reduction in osteocytes' induction of osteoclast activity. Endocortical bone formation also increased with treatment independently of exercise, providing gains in mechanical strength and tissue level properties. Overall, inhibiting P2Y2 activation has a beneficial effect on bone formation and the anabolic response to loading, namely by limiting osteoclast activation.
How Does Humanoid Virtual Influencers' Appearance Convey Social Presence? The Underlying Process and Path to Purchase Intention
Authors: Zourrig Haithem , Park Jeongsoo , Becheur Imene 
Published: January 2025

ABSTRACTDrawing on the stimulus‐organism‐response (S‐O‐R) framework, the current work contends that the interplay between Humanoid Virtual Influencer (HVI) traits acts as a stimulus that shapes consumers' sense of social presence and perception of HVI's attractiveness, which in turn influences their willingness to follow HVI's recommendations and purchase intention. Four online survey studies were conducted among Instagram users in Japan. The results from Study 1 show that animism partially mediates the impact of HVI anthropomorphism on social presence. Moreover, Study 2 reveals that warmth and competence dimensions, as stereotypical evaluations, partially mediate the influence of anthropomorphism and animism on social presence. Unexpectedly, Study 3 found that HVI attractiveness does not mediate the linkage between social presence and consumers' disposition to follow HVI recommendations. However, Study 4 reveals that the disposition to follow HVI recommendations acts as a mediating factor in the pathway between attractiveness and the intention to make a purchase. This research expands the existing literature by considering animism as an additional characteristic of HVIs and examining its influence on stereotypical evaluations. These insights provide practical implications for retail marketers who collaborate with HVIs in their advertising efforts.
Exploring the competencies of nephrology nurses: A comprehensive scoping review
Authors: Andreoli Desirèe , Morales Palomares Sara , Mancin Stefano , Parozzi Mauro , Gazineo Domenica , Palmisano Addolorata , Angileri Salvatore Alessio , Ricco Mattia , Anastasi Giuliano , Savini Serenella , Cangelosi Giovanni , Godino Lea , Sguanci Marco 
Published: January 2025

AbstractBackgroundChronic kidney disease (CKD) presents a significant global health challenge. Nephrology nurses, possessing specialized competencies, play an essential role in providing high‐quality care to CKD patients.AimThis scoping review aims to comprehensively map and synthesize literature on the competencies of nephrology nurses worldwide.MethodsA scoping review was reported using the PRISMA‐ScR framework and aligned with the Joanna Briggs Institute (JBI) methodology. The population, concept, and context (PCC) model was employed to formulate the research question. The search was conducted between June and July 2023 across five databases:Medline, Scopus, Cochrane Library, ASSIA, and CINAHL, with additional consultation of gray literature sources. Screening of articles, data extraction, and quality appraisal were independently performed by two authors. Critical appraisal was conducted using JBI critical appraisal tools. Data were analyzed using descriptive analysis.ResultsOf the 8786 records identified, 25 were included. The included articles predominantly consisted of position papers published between 2000 and 2022, with studies conducted across all continents. Core competencies for nephrology nurses were identified, along with advanced competencies encompassing advanced clinical practice, education, leadership and management, and research.ConclusionThe review of competencies among nephrology nurses worldwide reveals a comprehensive and diverse set of skills required to address the complexities of CKD. As the field of nephrology continues to evolve, ongoing efforts should be directed toward refining and expanding the competencies of nephrology nurses in individual national contexts as well as defining, in the light of a systematic mapping of skills, homogeneous training paths.Implications for nursingThis scoping review aims to synthesize global literature on nephrology nurses' core and advanced skills, providing a foundation for defining competencies at both national and institutional levels.
Hot‐Air‐Assisted Infrared Drying for Marinated Anchovy: Effects of Spice Blends on Drying Characteristics and Quality
Authors: Neethu K. C. , Aniesrani Delfiya D. S. , Remya S. , Murali S. , Ninan George 
Published: January 2025

ABSTRACTThis study examined the drying characteristics and quality of raw and marinated anchovy using a hot‐air‐assisted pilot‐scale infrared (IR) dryer. Anchovy was marinated with various Indian spice blends and dried for 3 h under controlled conditions. Four marinade formulations were tested: M1 (0.5% salt, 0.25% red chili powder, 0.25% turmeric powder), M2 (1% salt, 0.25% red chili powder, 0.25% turmeric powder), M3 (1% salt, 0.5% red chili powder, 0.5% turmeric powder), and M4 (0.5% salt, 0.5% red chili powder, 0.5% turmeric powder), along with a control. Both marination and drying time significantly affected the final moisture content (p ≤ 0.001). The highest drying rate was observed for M3 (1.442 g/g dry matter/h) and the lowest for raw anchovy (1.389 g/g dry matter/h). Tukey's analysis revealed significant differences in drying rates between M3 and other marinated samples, as well as between raw and certain marinated samples, highlighting the effect of marination on drying efficiency. The diffusion approach model worked best with M3 anchovy. Effective moisture diffusivity values ranged from 7.003 × 10−9 to 8.913 × 10−9 m2/s, with a highest value reported for M3. The results signify the opportunities to improve the hot‐air‐assisted infrared (IR‐HA) drying process and potentially reduce drying time and certainly energy usage. The findings emphasize that spice blends influence both drying characteristics and sensory attributes, offering valuable insights for refining marination strategies to enhance flavor, consistency, and product quality. Marinating before drying is recommended as a method to add the value to anchovy. The economic analysis revealed that the payback period for the IR‐HA dryer when used to dry marinated anchovy is 0.88 years.
Global Burden, Trends, and Inequalities of Gallbladder and Biliary Tract Cancer, 1990–2021: A Decomposition and Age–Period–Cohort Analysis
Authors: Lei Sen , Huang Guizhong , Li Xiaohui , Xi Pu , Yao Zehui , Lin Xiaojun 
Published: January 2025

ABSTRACTBackgroundGallbladder and biliary tract cancer (GBTC) increasingly aggravates the global malignancy burden. This study aimed to evaluate the updated condition of GBTC temporal burden trends and inequalities from 1990 to 2021.MethodsData on GBTC were extracted from the Global Burden of Disease (GBD) 2021 study. Incidence, deaths, and disability‐adjusted life years (DALYs) and their age‐standardised rates (ASR) were quantified from 1990 to 2021, stratified by sex, age and sociodemographic index (SDI). The age–period–cohort (APC) model was used to elucidate the effects of age, period, and cohort. Decomposition analysis and cross‐country inequality evaluation were performed to assess the contributing factors and disease imbalance, respectively. Bayesian APC analysis was used to estimate the future burden.ResultsIn 2021, the global incident cases of GBTC were 216 768, with 171 961 deaths and 3 732 121 DALYs lost. From 1990 to 2021, the ASR of incidence, mortality, and DALYs decreased slightly. Males showed a slight increase in ASR of incidence, while females experienced a significant decrease. High‐income regions, particularly in Asia Pacific and Latin America, exhibited a higher burden, while Western Sub‐Saharan Africa had the lowest. Low and low‐middle SDI regions showed a gradual rise in all metrics despite lower absolute numbers. The APC analysis indicated that the global incidence of GBTC tended to rise with age, but gender differences existed. Besides, a deteriorating cohort effect was detected amongst individuals born between 1907 and 1917. Decomposition analysis revealed that population growth was the primary driver of the increased GBTC burden globally. Significant disparities in GBTC burden by SDI were observed, with a notable decline in inequality over time. Projections indicated a slow decline in the global ASR through 2040, with a more pronounced decrease in females.ConclusionsThere are significant regional and gender differences in the global burden of GBTC. Population growth remains a major contributor to the burden. Despite the overall decline, the increasing incidence in low and lower‐middle SDI regions and the persistent male burden highlight the need for targeted interventions. Future efforts should focus on addressing socio‐economic inequalities and reducing risk factors, particularly in vulnerable populations.