bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of high‐performance adsorbents for separation of CO2 from industrial effluent gases
Authors: Hedayati Ali , Delica Beñat Anda , Perez‐Gil Susana , Prieto‐Fernandez Soraya 
Published: December 2022

AbstractThe aim of this work is the investigation of several CO2 adsorbents such as three synthetic zeolites (4A, 5A, and 13X), a natural zeolite (clinoptilolite), an activated carbon (AC), and a metal organic framework (MOF‐MIL‐53) for the postcombustion CO2 capture via temperature‐pressure swing adsorption. The adsorbents were evaluated in terms of the CO2 adsorption capacity and performance during successive adsorption–desorption cycles at different conditions (1–6 bar and 120–180°C) using different mixtures of gases simulating a combustion process stream (N2/CO2/O2/CO). The 13X zeolite showed excellent adsorption capacity of 4.3 mmolCO2/g zeolite and very stable performance during successive cycles. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
Epigenetic alterations in glioblastomas: Diagnostic, prognostic and therapeutic relevance
Authors: Montella Liliana , Cuomo Mariella , Del Gaudio Nunzio , Buonaiuto Michela , Costabile Davide , Visconti Roberta , Di Risi Teodolinda , Vinciguerra Roberta , Trio Federica , Ferraro Sara , Bove Guglielmo , Facchini Gaetano , Altucci Lucia , Chiariotti Lorenzo , Della Monica Rosa 
Published: December 2022

AbstractGlioblastoma, the most common and heterogeneous tumor affecting brain parenchyma, is dismally characterized by a very poor prognosis. Thus, the search of new, more effective treatments is a vital need. Here, we will review the druggable epigenetic features of glioblastomas that are, indeed, currently explored in preclinical studies and in clinical trials for the development of more effective, personalized treatments. In detail, we will review the studies that have led to the identification of epigenetic signatures, IDH mutations, MGMT gene methylation, histone modification alterations, H3K27 mutations and epitranscriptome landscapes of glioblastomas, in each case discussing the corresponding targeted therapies and their potential efficacy. Finally, we will emphasize how recent technological improvements permit to routinely investigate many glioblastoma epigenetic biomarkers in clinical practice, further enforcing the hope that personalized drugs, targeting specific epigenetic features, could be in future a therapeutic option for selected patients.
The multifaceted impact of US trade policy on financial markets
Authors: Boer Lukas , Menkhoff Lukas , Rieth Malte 
Published: December 2022

SummaryWe study the multifaceted effects of trade policy shocks on financial markets using a structural vector autoregression identified via event day heteroskedasticity. We find that restrictive US trade policy shocks affect US and international stock prices heterogeneously, but generally negatively. They increase market uncertainty, lower US interest rates, and lead to an appreciation of the US dollar. The effects are significant for several weeks or quarters. Decomposing the trade policy shocks further suggests that trade policy uncertainty dominates tariff level effects. Chinese trade policy shocks against the United States further hurt US stocks.
Decellularization of cartilage microparticles: Effects of temperature, supercritical carbon dioxide and ultrasound on biochemical, mechanical, and biological properties
Authors: Sevastianov Victor I. , Basok Yulia B. , Grigoriev Alexey M. , Nemets Evgeny A. , Kirillova Alexandra D. , Kirsanova Liudmila A. , Lazhko Aleksey E. , Subbot Anastasia , Kravchik Marina V. , Khesuani Yusef D. , Koudan Elizaveta V. , Gautier Sergey V. 
Published: December 2022

AbstractOne of the approaches to restoring the structure of damaged cartilage tissue is an intra‐articular injection of tissue‐engineered medical products (TEMPs) consisting of biocompatible matrices loaded with cells. The most interesting are the absorbable matrices from decellularized tissues, provided that the cellular material is completely removed from them with the maximum possible preservation of the structure and composition of the natural extracellular matrix. The present study investigated the mechanical, biochemical, and biological properties of decellularized porcine cartilage microparticles (DCMps) obtained by techniques, differing only in physical treatments, such as freeze–thaw cycling (Protocol 1), supercritical carbon dioxide fluid (Protocol 2) and ultrasound (Protocol 3). Full tissue decellularization was achieved, as confirmed by the histological analysis and DNA quantification, though all the resultant DCMps had reduced glycosaminoglycans (GAGs) and collagen. The elastic modulus of all DCMp samples was also significantly reduced. Most notably, DCMps prepared with Protocol 3 significantly outperformed other samples in viability and the chondroinduction of the human adipose‐derived stem cells (hADSCs), with a higher GAG production per DNA content. A positive ECM staining for type II collagen was also detected only in cartilage‐like structures based on ultrasound‐treated DCMps. The biocompatibility of a xenogenic DCMps obtained with Protocol 3 has been confirmed for a 6‐month implantation in the thigh muscle tissue of mature rats (n = 18). Overall, the results showed that the porcine cartilage microparticles decellularized by a combination of detergents, ultrasound and DNase could be a promising source of scaffolds for TEMPs for cartilage reconstruction.
Large scale peptide screening against main protease of SARS CoV‐2
Authors: Uddin Md. Jaish , Akhter Hasina , Chowdhury Urmi , Mawah Jannatul , Karim Sanzida Tul , Jomel Mohammad , Islam Md. Sirajul , Islam Mohammad Raqibul , Onin Latifa Afrin Bhuiyan , Ali Md. Ackas , Efaz Faiyaz Md. , Halim Mohammad A. 
Published: December 2022

AbstractThe COVID‐19 pandemic has been a public health emergency, with deadly forms constantly emerging around the world, highlighting the dire need for highly effective antiviral therapeutics. Peptide therapeutics show significant potential for this viral disease due to their efficiency, safety, and specificity. Here, two thousand seven hundred eight antibacterial peptides were screened computationally targeting the Main protease (Mpro) of SARS CoV‐2. Six top‐ranked peptides according to their binding scores, binding pose were investigated by molecular dynamics to explore the interaction and binding behavior of peptide‐Mpro complexes. The structural and energetic characteristics of Mpro‐DRAMP01760 and Mpro‐DRAMP01808 complexes fluctuated less during a 250 ns MD simulation. In addition, three peptides (DRAMP01760, DRAMP01808, and DRAMP01342) bind strongly to Mpro protein, according to the free energy landscape and principal component analysis. Peptide helicity and secondary structure analysis are in agreement with our findings. Interaction analysis of protein‐peptide complexes demonstrated that Mpro's residue CYS145, HIS41, PRO168, GLU166, GLN189, ASN142, MET49, and THR26 play significant contributions in peptide‐protein attachment. Binding free energy analysis (MM‐PBSA) demonstrated the energy profile of interacting residues of Mpro in peptide‐Mpro complexes. To summarize, the peptides DRAMP01808 and DRAMP01760 may be highly Mpro specific, resulting disruption in a viral replication and transcription. The results of this research are expected to assist future research toward the development of antiviral peptide‐based therapeutics for Covid‐19 treatment.
A novel biomarker for pleural effusion diagnosis: Interleukin‐36γ in pleural fluid
Authors: Guo Lun , Zhang Qipan , Lv Chengna , Ma Xudan , Song Xuxiang , Huang Jing , Chen Weili , Li Chaofen , Ding Qunli 
Published: December 2022

AbstractBackgroundNumerous studies have described the critical importance of interleukin (IL) ‐36γ in host defense against lung infections, but it is unknown whether it plays a role in infectious pleural effusion (IPE). This study aimed to examine the levels of IL‐36γ in pleural effusions of different etiologies and evaluate the diagnostic accuracy of IL‐36γ in the differential diagnosis of IPE.MethodsA total of 112 individuals was enrolled in this research. IL‐36γ levels in pleural fluids of all 112 patients were measured by enzyme‐linked immunosorbent assay (ELISA). We also characterized these markers' diagnostic values across various groups.ResultsPatients with tuberculous pleural effusion (TPE) and parapneumonic effusion (PPE) had exhibited markedly higher IL‐36γ levels in their pleural fluid than the malignant pleural effusion (MPE) and transudative effusion patients. Furthermore, the IL‐36γ concentrations in TPE patients were evidently higher than in uncomplicated parapneumonic effusion (UPPE) patients but significantly lower than in complicated parapneumonic effusion (CPPE)/empyema patients. Pleural fluid IL‐36γ is a useful marker to differentiate TPE from UPPE, at a cut‐off value for 657.5 pg/ml (area under the curve = 0.904, p < 0.0001) with 70.0% sensitivity and 95.7% specificity.ConclusionsThe elevated IL‐36γ in pleural effusion may be used as a novel biomarker for infectious pleural effusion diagnosis, particularly in patients with CPPE/empyema, and is a potentially promising biomarker to differentiate between TPE and UPPE.
Mendelian randomisation studies of Attention Deficit Hyperactivity Disorder
Authors: Riglin Lucy , Stergiakouli Evie 
Published: December 2022

AbstractBackgroundObservational studies have found Attention Deficit Hyperactivity Disorder (ADHD) to be associated with an increased risk of adverse outcomes as well as with early risk factors; however it is not clear whether these associations reflect causal effects. Alternatives to traditional observational studies are needed to investigate causality: one such design is Mendelian randomization (MR), which uses genetic variants as instrumental variables for the exposure.MethodsIn this review we summarise findings from approximately 50 studies using MR to examine potentially causal associations with ADHD as either an exposure or outcome.ResultsTo‐date, few MR ADHD studies have investigated causal evidence with other neurodevelopmental, mental health and neurodegenerative conditions but those that have suggest a complex relationship with autism, some evidence of a causal effect on depression and limited evidence of a causal effect on neurodegenerative conditions. For substance use, MR studies provide evidence consistent with a causal effect of ADHD on smoking initiation, but findings for other smoking behaviours and cannabis use are less consistent. Studies of physical health suggest bidirectional causal effects with higher body mass index, with stronger effects for childhood obesity, as well as some evidence of causal effects on coronary artery disease and stroke in adults and limited evidence of causal effects on other physical health problems or sleep. Studies suggest bidirectional relationships between ADHD and socio‐economic markers and provide some evidence that low birthweight may be a causal risk factor for ADHD, while bidirectional evidence has been found for some environmental factors. Finally, there is emerging evidence of bidirectional causal links between ADHD genetic liability and biological markers of human metabolism and inflammation.ConclusionsWhile MR has advantages over traditional observational designs in addressing causality, we discuss limitations of current ADHD studies and future directions, including the need for larger genome‐wide association studies (and using samples of different ancestries), and for triangulation with different methods.
Healthcare decision makers' perspectives on the creation of new genetic counselor positions in North America: Exploring the case for psychiatric genetic counseling
Authors: Chanouha Nour , Cragun Deborah L. , Pan Vivian Y. , Austin Jehannine C. , Hoell Christin 
Published: December 2022

AbstractMental illnesses are common and highly heritable. Patients and their families want and benefit from receiving psychiatric genetic counseling (pGC). Though the pGC workforce is among the smallest of genetic counseling (GC) specialties, genetic counselors (GCs) want to practice in this area. A major barrier to the expansion of the pGC workforce is limited availability of advertised positions, but it remains unclear why this is the case. We used a qualitative approach to explore drivers for and barriers to the creation of GC positions (including pGC) at large centralized genetic centers in the United States and Canada that offer multiple specialty GC services. Individuals with responsibilities for making decisions about creating new clinical GC positions were interviewed using a semi‐structured guide, and an interpretive description approach was used for inductive data analysis. From interviews with 12 participants, we developed a theoretical model describing how the process of creating new GC positions required institutional prioritization of funding, which was primarily allocated according to physician referral patterns, which in turn were largely driven by availability of genetic testing and clinical practice guidelines. Generating revenue for the institution, improving physician efficiency, and reinforcing institutional mission were all regarded as valued outcomes that bolstered prioritization of funding for new GC positions. Evidence of patient benefit arising from new GC positions (e.g., pGC) seemed to play a lesser role. These findings highlight the tension between how institutions value GC (generating revenue, reacting to genetic testing), and how the GC profession sees its value (providing patient benefit, focus on counseling).
Patient uptake of updated genetic testing following uninformative BRCA1 and BRCA2 results
Authors: Macklin‐Mantia Sarah K. , Clift Kristin E. , Maimone Santo , Hodge David O. , Riegert‐Johnson Douglas , Hines Stephanie L. 
Published: December 2022

AbstractAdvances have dated the genetic testing initially offered to evaluate for hereditary breast and ovarian cancer risks. Previous research has demonstrated that many patients have not updated testing. This study reviewed the incidence of additional analysis after an uninformative BRCA1/2 result and offered updated testing with limited barriers to those who had not completed. After viewing an educational video and providing informed consent, eligible patients were mailed a saliva collection kit to complete an 84‐gene hereditary cancer panel at no personal cost. A total of 704 patients had completed BRCA1/2 only testing between 2001 and 2020. Fifteen percent (N = 102) of the 671 patients with an uninformative BRCA1/2 result had already completed expanded testing. Most, 74 of 102 (73%), had been rereferred to medical genetics during a clinical visit related to cancer care. Those who had already completed additional testing were more likely to have a personal history of cancer (92% vs. 79%, p = 0.002) and live locally (p = 0.032). Invitation to complete updated testing through this study was sent to 372 people, and 116 (31%) consented to participate. For 142 of the 256 who did not proceed with testing through the study, proof of receipt of research information was available. In total, 22 pathogenic variants were reported in 21 of the 226 patients with updated testing from before and including our study: ATM (4), CHEK2 (4), LZTR1 (1), MUTYH (3), NBN (1), NF1 (1), NTHL1 (1), PALB2 (4), PMS2 (1), RAD50 (1), and SPINK1 (1). Many potential barriers of retesting were eliminated by removing personal costs or travel requirements. Still, only about 30% of patients agreed to participate, and a significant portion elected not to proceed. Future research could focus on the discovery of other factors that dissuade patients and what measures may better inform them on potential benefits.
Intersection of professional identity development and experiences of cultural insensitivity among international counselor education doctoral students
Authors: Kuo Patty , Washington Ahmad , Woo Hongryun 
Published: December 2022

AbstractThe authors used a qualitative research method to examine experiences of cultural insensitivity and their influences on professional identity among 13 international counselor education students. Participants described experiencing discrimination and how these experiences pushed them to incorporate advocacy into their professional identities. Participants also described the impact of discrimination on their cultural identities and the lack of global perspectives in mental health professions. Implications for counselor education are discussed.
Humoral and cellular immunity of two‐dose inactivated COVID‐19 vaccination in Chinese children: A prospective cohort study
Authors: Wang Hao , Gan Mengze , Wu Bihao , Zeng Rui , Wang Zhen , Xu Jun , Li Jia , Zhang Yandi , Cao Jinge , Chen Li , Di Dongsheng , Peng Siyuan , Lei Jinfeng , Zhao Yingying , Song Xuemei , Yuan Tingting , Zhou Tingting , Liu Qian , Yi Jing , Wang Xi , Cai Hao , Lei Yanshou , Wen Yuying , Li Wenhui , Chen Qinlin , Wang Yufei , Long Pinpin , Yuan Yu , Wang Chaolong , Pan An , Wang Qi , Gong Rui , Fan Xionglin , Wu Tangchun , Liu Li 
Published: December 2022

AbstractChildren are the high‐risk group for COVID‐19, and in need of vaccination. However, humoral and cellular immune responses of COVID‐19 vaccine remain unclear in vaccinated children. To establish the rational immunization strategy of inactivated COVID‐19 vaccine for children, the immunogenicity of either one dose or two doses of the vaccine in children was evaluated. A prospective cohort study of 322 children receiving inactivated COVID‐19 vaccine was established in China. The baseline was conducted after 28 days of the first dose, and the follow‐up was conducted after 28 days of the second dose. The median titers of receptor binding domain (RBD)‐IgG, and neutralizing antibody (NAb) against prototype strain and Omicron variant after the second dose increased significantly compared to those after the first dose (first dose: 70.0, [interquartile range, 30.0–151.0] vs. second dose: 1261.0 [636.0–2060.0] for RBD‐IgG; 2.5 [2.5–18.6] vs. 252.0 [138.6–462.1] for NAb against prototype strain; 2.5 [2.5–2.5] vs. 15.0 [7.8–26.5] for NAb against Omicron variant, all p < 0.05). The flow cytometry results showed that the first dose elicited SARS‐CoV‐2 specific cellular immunity, while the second dose strengthened SARS‐CoV‐2 specific IL‐2+ or TNF‐α+ monofunctional, IFN‐γ+TNF‐α+ bifunctional, and IFN‐γ−IL‐2+TNF‐α+ multifunctional CD4+ T cell responses (p < 0.05). Moreover, SARS‐CoV‐2 specific memory T cells were generated after the first vaccination, including the central memory T cells and effector memory T cells. The present findings provide scientific evidence for the vaccination strategy of the inactive vaccines among children against COVID‐19 pandemic.
Hyperspectral technique combined with stacking and blending ensemble learning method for detection of cadmium content in oilseed rape leaves
Authors: Cheng Jiehong , Sun Jun , Yao Kunshan , Xu Min , Wang Simin , Fu Lvhui 
Published: December 2022

AbstractBackgroundOilseed rape, as one of the most important oil crops, is an important source of vegetable oil and protein for mankind. As a non‐essential element for plant growth, heavy metal cadmium (Cd) is easily absorbed by plants. Cd will inhibit the photosynthesis of plants, destroy the cell structure, slow the growth of plants, and affect their development and yield. It is necessary to develop a method based on visible near‐infrared (NIR) hyperspectral imaging (HSI) technology to quickly and nondestructively determine the Cd content in rape leaves.ResultsTwo‐layer estimation models were established by combining visible–NIR HSI with ensemble learning methods (stacking and blending). One layer used support vector regression, extreme learning machine, decision tree, and random forest (RF) as basic learners, and the other layer used support vector regression or RF as a meta learner. Different models were used to analyze the spectra of rape treated with five Cd concentrations to obtain the best prediction method. The results showed that the best model to predict Cd content was the stacking ensemble model with RF as the meta learner, with coefficient of determination for prediction of 0.9815 and root‐mean‐square error for prediction of 5.8969 mg kg−1. A pseudo‐color image was developed using this stacking model to visualize the content and distribution of Cd.ConclusionThe combination of visible–NIR HSI technology and the stacking ensemble learning method is a feasible method to detect the Cd content in rape leaves, which has the potential of being rapid and nondestructive. © 2022 Society of Chemical Industry.
Endoscopic Reconstruction of the Anterior Skull Base Following Tumor Resection: Application of a Novel Bioabsorbable Plate
Authors: Piscopo Anthony J. , Dougherty Mark C. , Woodiwiss Timothy R. , Ankrah Nii‐Kwanchie , Hughes Tyler , Seaman Scott C. , Walsh Jarrett E. , Graham Scott M. , Greenlee Jeremy D. W. 
Published: December 2022

ObjectiveEndoscopic repair of skull base defects is required following resection of intracranial pathology via the endoscopic endonasal approach (EEA). Many closure techniques have been described, but choosing between techniques remains controversial. We report outcomes of 560 EEA procedures of skull base reconstruction performed on 508 patients over a 15‐year‐period. Halfway through this period, we adopted the use of a rigid, bioabsorbable extrasellar plate for reconstruction, enabling a comparison between this technique and those used previously.MethodsAll patients undergoing EEA from 2005 to 2019 at our institution were retrospectively reviewed. Demographic information, surgical pathology, tumor dimensions and radiographic features, reconstructive technique, and patient‐related outcomes were collected and analyzed with univariate and multivariate statistical modeling.ResultsFive‐hundred sixty procedures were performed on 508 patients. The series complication rate was 8.2%. Overall, cerebrospinal fluid (CSF) leak rate was 5.0% but varied significantly across closure techniques (p < 0.001). Critically, the CSF leak rate in the 272 cases prior to our 2013 adoption of the Resorb‐X Plate (RXP) was 8.5%, whereas leak rate in the subsequent 288 cases was 1.7%. RXP was protective against CSF leak (p = 0.001), whereas gross total resection (GTR) correlated with increased leak rate (p = 0.001). Patient BMI was significantly associated with risk of leak (p = 0.047). Other variables did not impact leak risk.ConclusionReconstructive technique, extent of resection, and patient BMI significantly contributed to CSF leak rate. GTR was associated with increased leak risk while the RXP was protective. The bioabsorbable RXP is an effective option for rigid skull base repair with comparatively few complications.Level of Evidence3 Laryngoscope, 133:1092–1098, 2023