bluuebunny/crossref_metadata_2025_split huggingface dataset:
Female‐induced selective modification of sperm protein SUMOylation—potential mechanistic insights into the non‐random fertilization in humans
Authors: Kekäläinen Jukka , Hiltunen Johannes , Jokiniemi Annalaura , Kuusipalo Liisa , Heikura Marjo , Leppänen Jonna , Malinen Marjo 
Published: January 2022

AbstractIn many species, mate choice continues after the mating via female‐ or egg‐derived biochemical factors that induce selective changes in sperm pre‐fertilization physiology and behaviour. Recent studies have indicated that gamete‐mediated mate choice likely occurs also in humans, but the mechanistic basis of the process has remained virtually unexplored. Here, we investigated whether female‐induced modifications in sperm protein SUMOylation (post‐translational modification of the proteome) could serve as a novel mechanism for gamete‐mediated mate choice in humans. We treated the sperm of ten males with the oocyte‐surrounding bioactive liquid (follicular fluid) of five females and investigated motility, viability and global protein SUMOylation status of the sperm in all (n = 50) of these male–female combinations (full‐factorial design). All the measured sperm traits were affected by male–female combinations, and sperm protein SUMOylation status was also negatively associated with sperm motility. Furthermore, our results indicate that female‐induced sperm protein SUMOylation is selective, potentially allowing females to increase sperm motility in some males, whereas decreasing it in the others. Consequently, our findings suggest that follicular fluid may non‐randomly modify the structure and function of sperm proteome and in this way facilitate gamete‐mediated mate choice in humans and possibly many other species. However, due to the relatively low number of female subjects and their potential infertility problems, our results should be replicated with larger subset of fully fertile women.
Evolution of the stock of electrical and electronic equipment in the Peruvian residential sector
Authors: Gusukuma Marco , Kahhat Ramzy , Cáceres Kathia 
Published: January 2022

AbstractConsumption of appliances in the residential sector in Peru has been growing continuously during the last 20 years. Although social benefits due to this growth are evident, there are also some related environmental impacts in the use and end‐of‐life (EoL) phases (e.g., inadequate handling or disposal at the EoL stage). Nevertheless, there is also a hidden potential in the growing stock of household appliances, such as their potential exploitation as resources of industrial materials found in urban areas. Thus, the aim of this research paper is to analyze the evolution of the adoption of electronics in Peruvian households and estimate the stock of electrical and electronic equipment and related materials in the residential sector from 2001 to 2019, and greenhouse gases (GHG) emissions due to its use. Material flow analysis is the main methodology used in this research and its application relies on different strategies and the integrated use of official sources. Moreover, a Peruvian input–output table and associated environmental matrices were used to calculate GHG emissions. Results indicate that, in 2019, an average household possessed between 86 and 121 kg of appliances, which means the total stock of household appliances in Peru was in the range of 805,000 to 1,134,000 metric tons, an increase in mass of 70–95% by 2019 compared to 2001. These results will be useful to estimate the urban stock of appliances in the residential sector to help policy‐makers design and implement an adequate e‐waste management system that comprehends the potential of secondary materials embedded in these products.
Treatment of neuroendocrine tumours with 177Lu‐peptide receptor radionuclide therapy: Challenging clinical scenarios and their management
Authors: Navalkissoor Shaunak , Toumpanakis Christos , Caplin Martyn , Gnanasegaran Gopinath 
Published: January 2022

AbstractPeptide receptor radionuclide therapy (PRRT) for the treatment of patients with neuroendocrine tumours is usually well tolerated, with selection based on existing guidelines. However, there are various scenarios where the risk–benefit analysis of PRRT needs to be evaluated to limit any complications associated with PRRT. This review looks at 11 key scenarios where the risk–benefit of PRRT needs to be closely evaluated.
Oral health‐related quality of life in patients with Parkinson’s disease
Authors: Verhoeff Merel C. , Lobbezoo Frank , van Leeuwen Astrid M. , Schuller Annemarie A. , Koutris Michail 
Published: January 2022

AbstractBackgroundParkinson's disease (PD) is a neurodegenerative condition affecting the quality of life. Due to a worsening of oral health in PD patients with the progression of the disease, oral health‐related quality of life (OHRQoL) could be impaired as well.ObjectivesTo assess whether PD patients in The Netherlands experience worse OHRQoL than historical controls, and to investigate which factors are associated with OHRQoL in PD patients.Materials & MethodsIn total, 341 PD patients (65.5 ± 8.4 years) and 411 historical controls (62.6 ± 5.3 years) participated. Both groups completed a questionnaire. The PD patients were asked questions regarding demographics, PD, oral health, and OHRQoL. The historical controls filled in demographic information and questions regarding OHRQoL. The latter construct was assessed using the Dutch 14‐item version of the Oral Health Impact Profile (OHIP‐14). Data were analysed using independent samples t‐tests and univariate and multivariate linear regression analysis.ResultsThe mean OHIP‐14 score was higher in PD patients (19.1 ± 6.7) than in historical controls (16.5 ± 4.4) (t(239) = 6.5; p < .001). OHRQoL in PD patients was statistically significant associated with motor aspects of experiences of daily living (B = 0.31; t(315) = 7.03; p < .001), worsening of the oral environment during disease course (B = 3.39; t(315) = 4.21; p < .001), being dentate (B = −5.60; t(315) = −4.5; p < .001), tooth wear (B = 2.25; t(315) = 3.29; p = .001), and possible burning mouth syndrome (B = 5.87; t(315) = 2.87; p = .004).ConclusionPD patients had a lower OHRQoL than historical controls. Besides, PD‐related variables and oral health‐related variables were associated with OHRQoL.
Management of Fused Anterior Teeth: Review and Clinical Report
Authors: Chen Fangbing , Wang Shaolei , Bai Na , Li Xue , Wang Lizhe , Xia Xiaomin , Liu Jie , Tan Fei 
Published: January 2022

AbstractThe phenomenon of fusion is a type of tooth dysplasia, but few studies have systematically described the treatment of this kind of abnormality. This paper summarizes the treatment methods for fused teeth and classifies the management schemes according to whether the pulp is fused. Then, the treatment for a patient with bilateral anterior tooth fusion is reported. After orthodontic treatment, porcelain veneers were used to restore the normal shape and aesthetic appearance of the anterior teeth.
Clinical Evaluation of Pink Esthetic Score of Immediately Impressed Posterior Dental Implants
Authors: Genetti Loren , Ercoli Carlo , Kotsailidi Elli Anna , Feng Changyong , Tsigarida Alexandra , Russo Lucio Lo , Chochlidakis Konstantinos 
Published: January 2022

AbstractPurposeWhile comparative outcome results for peri‐implant crestal bone levels, mucosal margin position, and peri‐implant indices have been reported, no studies are available that evaluate and compare the esthetic result of impressions performed immediately at implant placement with that of impressions performed on healed implants. The purpose of this cross‐sectional study was to evaluate the pink esthetic score of posterior implants restored with an immediate impression workflow compared to implants restored with a delayed impression workflow.Material and MethodsTwenty‐eight eligible participants who had received a single implant crown either in the premolar or molar regions at least 4 months before the study, were identified by an electronic health record review and scheduled for a single‐visit study appointment. Esthetic outcomes were measured using the pink esthetic score. Several local‐ and prosthesis‐related factors were recorded and their association with the selected outcome was assessed. Two‐sample t‐test was used for comparisons between the groups.ResultsPink esthetic score ranged between 4 and 12 (mean: 8). No significant difference between immediate (8.36 ±1.12) and delayed (7.76 ±2.14) impression workflow groups were seen for the total PES (p = 0.25). In addition, individual comparisons between immediately and delayed impressed implants for mesial papilla (1.27 ±0.47; 0.88 ±0.78), distal papilla (0.73 ±0.65; 0.76 ±0.83), tissue margin (1.73 ±0.47; 1.47 ±0.51), tissue contour (1.27 ±0.65; 0.82 ±0.64), alveolar process (0.82 ±0.60; 1.00 ±0.87), color (1.27 ±0.65; 1.29 ±0.69), and texture (1.36 ±0.50; 1.53 ±0.62) did not show significant differences.ConclusionsThe current study suggests that the pink esthetic score is not significantly different between single posterior implants impressed with immediate and delayed implant workflows.
Effect of horticultural therapy on mental health: A meta‐analysis of randomized controlled trials
Authors: Tu Hung‐Ming 
Published: January 2022

Accessible summaryWhat is known on the subject?

Mental health assessment is a common topic in horticultural therapy (HT).

Some study findings were contrary to positive findings, although several studies have explored the effect of HT on mental health and reported positive results.

There is weak evidence in the benefits of HT based on evidence‐based medicine. Stronger evidence of the link between HT and mental health will help in policy making by health policy makers and governments.

What the paper adds to existing knowledge?

The purpose of this meta‐analysis of randomized controlled trials (RCTs) was to explore the effect of HT on mental health.

This study is the first meta‐analysis of RCTs to provide critical evidence that HT has a positive effect on mental health.

The effect size of HT on mental health was medium (0.55). The included studies had no negative effects on mental health.

What are the implications for practice?

HT should be considered a useful therapy to be integrated in healthcare settings by horticultural therapists to improve mental health.

In the HT program, indoor and outdoor plant activities, plant‐related arts, crafts and other activities were common interventions to improve specific mental health problems through at least eight sessions.

AbstractIntroductionHT has been widely used to promote mental health. However, heterogeneity and sample size issues of randomized controlled trials made it challenging to illustrate effect sizes across the evidence.AimThe purpose of this meta‐analysis was to explore the effect of HT on mental health.MethodsWe used the PRISMA framework. A keyword search of Web of Science, PubMed, ProQuest and Cochrane was performed. The inclusion criteria were HT with RCTs and mental health assessments. A random‐effects model was used to perform the meta‐analysis.ResultsA total of 1,056 records were searched, and 18 eligible studies were extracted. The included RCTs had no statistical heterogeneity and publication bias. The meta‐analysis showed that the HT experimental groups had a significant and positive impact on mental health compared with the control groups (effect size = 0.55).DiscussionHT should be considered for enhancing mental health. The included studies had no negative outcomes and the most common HT intervention was at least eight sessions. Therefore, HT should be considered to enhance mental health. However, the concealed allocation and blinding processes should be improved in future studies.Implications for Practice: This study recommends that HT should be integrated into healthcare settings to improve mental health.
Extensive ethnolinguistic diversity at the crossroads of North China and South Siberia reflects multiple sources of genetic diversity
Authors: He Guang‐Lin , Wang Meng‐Ge , Zou Xing , Yeh Hui‐Yuan , Liu Chang‐Hui , Liu Chao , Chen Gang , Wang Chuan‐Chao 
Published: January 2022

AbstractNorth China and South Siberia, populated by Altaic‐ and Sino‐Tibetan‐speaking populations, possess extensive ethnolinguistic diversity and serve as the crossroads for the initial peopling of America and western–eastern transcontinental communication. However, the population genetic structure and admixture history of northern East Asians remain poorly understood due to a lack of genome‐wide data, especially for Mongolic‐speaking people in China. We genotyped genome‐wide single nucleotide polymorphisms for 510 individuals from 38 Mongolic, Tungusic, and Sinitic‐speaking populations. We first explored the shared alleles and haplotypes within the studied groups. We then merged with 3508 published modern and ancient Eurasian individuals to reconstruct the deep evolutionary and natural selection history of northern East Asians. We identified genetic substructures within Altaic‐speaking populations: Western Turkic people harbored more western Eurasian‐related ancestry; Northern Mongolic people in Siberia and eastern Tungusic people in Amur River Basin (ARB) possessed a majority of Neolithic ARB related ancestry; Southern Mongolic people in China possessed apparent genetic influence from Neolithic Yellow River Basin (YRB) farmers. Additionally, we found the differentiated admixture history between western and eastern Mongolians and geographically close Northeast Hans: the former received a genetic impact from western Eurasians, and the latter retained the primary Neolithic YRB and ARB ancestry. Moreover, we demonstrated that Kalmyk people from the northern Caucasus Mountains possessed a strong genetic affinity with Neolithic Mongolian Plateau (MP) people, supporting the hypothesis of their eastern Eurasian origin and long‐distance migration history. We also illuminated that historical pastoral empires in the MP contributed considerably to the gene pool of northern Mongolic people but rarely to the southern ones. We finally found natural selection signatures in Mongolians associated with alcohol metabolism. Our results demonstrated that the Neolithic ancestral sources from the MP or ARB played an important role in spreading Altaic populations and languages. The observed multisources of genetic diversity contributed significantly to the extensive ethnolinguistic diversity in northern East Asia.
In vitro probiotic and safety attributes of Bacillus spp. isolated from beebread, honey samples and digestive tract of honeybees Apis mellifera
Authors: Toutiaee S. , Mojgani N. , Harzandi N. , Moharrami M. , Mokhberosafa L. 
Published: January 2022

Abstract
Bacillus species isolated from honeybee Apis mellifera gut, honey and bee bread samples were characterized for their in vitro probiotic and safety attributes. Alpha and γ haemolytic cultures were tested for their antibiotic resistance, antibacterial spectrum, acid and bile tolerance, adhesion ability (auto-aggregation, co-aggregation and hydrophobicity) and phenol tolerance. Safety criteria included evaluation of virulence genes and cytotoxicity percentages. Bacillus isolates inhibited both Gram-positive and Gram-negative pathogens including Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus mutans, while none could inhibit Listeria monocytogenes. Among the isolates, Bacillus subtilis ZH05, ZB03 and ZG025 showed resistance to most of the tested antibiotics and were considered unsafe. B. subtilis (4) and B. licheniformis (1) tolerated acidic pH and bile conditions, never the less were more tolerant in simulated intestinal conditions vis-a-vis gastric conditions. In 0·5% phenol concentrations, B. licheniformis ZH02 showed highest growth, while, B. subtilis ZG029 demonstrated highest auto-aggregation (65 ± 4·6) and hydrophobicity (23 ± 3·6) percentages (P &lt; 0·05). The isolates lacked virulence genes (hblA, hblC, hblD, nhe, cytK and ces), and their cytotoxic percentage on Caco-2 cell lines was ˂15%. Overall, honeybees appear to be a good source of Bacillus species exhibiting typical in vitro probiotic properties, which could be of commercial interest.
Long‐distance migration is a major factor driving local adaptation at continental scale in Coho salmon
Authors: Rougemont Quentin , Xuereb Amanda , Dallaire Xavier , Moore Jean‐Sébastien , Normandeau Eric , Perreault‐Payette Alysse , Bougas Bérénice , Rondeau Eric B. , Withler Ruth E. , Van Doornik Donald M. , Crane Penelope A. , Naish Kerry A. , Garza John Carlos , Beacham Terry D. , Koop Ben F. , Bernatchez Louis 
Published: January 2022

AbstractInferring the genomic basis of local adaptation is a long‐standing goal of evolutionary biology. Beyond its fundamental evolutionary implications, such knowledge can guide conservation decisions for populations of conservation and management concern. Here, we investigated the genomic basis of local adaptation in the Coho salmon (Oncorhynchus kisutch) across its entire North American range. We hypothesized that extensive spatial variation in environmental conditions and the species’ homing behaviour may promote the establishment of local adaptation. We genotyped 7829 individuals representing 217 sampling locations at more than 100,000 high‐quality RADseq loci to investigate how recombination might affect the detection of loci putatively under selection and took advantage of the precise description of the demographic history of the species from our previous work to draw accurate population genomic inferences about local adaptation. The results indicated that genetic differentiation scans and genetic–environment association analyses were both significantly affected by variation in recombination rate as low recombination regions displayed an increased number of outliers. By taking these confounding factors into consideration, we revealed that migration distance was the primary selective factor driving local adaptation and partial parallel divergence among distant populations. Moreover, we identified several candidate single nucleotide polymorphisms associated with long‐distance migration and altitude including a gene known to be involved in adaptation to altitude in other species. The evolutionary implications of our findings are discussed along with conservation applications.
Biodiversity and vector‐borne diseases: Host dilution and vector amplification occur simultaneously for Amazonian leishmaniases
Authors: Kocher Arthur , Cornuault Josselin , Gantier Jean‐Charles , Manzi Sophie , Chavy Agathe , Girod Romain , Dusfour Isabelle , Forget Pierre‐Michel , Ginouves Marine , Prévot Ghislaine , Guégan Jean‐François , Bañuls Anne‐Laure , de Thoisy Benoît , Murienne Jérôme 
Published: January 2022

AbstractChanges in biodiversity may impact infectious disease transmission through multiple mechanisms. We explored the impact of biodiversity changes on the transmission of Amazonian leishmaniases, a group of wild zoonoses transmitted by phlebotomine sand flies (Psychodidae), which represent an important health burden in a region where biodiversity is both rich and threatened. Using molecular analyses of sand fly pools and blood‐fed dipterans, we characterized the disease system in forest sites in French Guiana undergoing different levels of human‐induced disturbance. We show that the prevalence of Leishmania parasites in sand flies correlates positively with the relative abundance of mammal species known as Leishmania reservoirs. In addition, Leishmania reservoirs tend to dominate in less diverse mammal communities, in accordance with the dilution effect hypothesis. This results in a negative relationship between Leishmania prevalence and mammal diversity. On the other hand, higher mammal diversity is associated with higher sand fly density, possibly because more diverse mammal communities harbor higher biomass and more abundant feeding resources for sand flies, although more research is needed to identify the factors that shape sand fly communities. As a consequence of these antagonistic effects, decreased mammal diversity comes with an increase of parasite prevalence in sand flies, but has no detectable impact on the density of infected sand flies. These results represent additional evidence that biodiversity changes may simultaneously dilute and amplify vector‐borne disease transmission through different mechanisms that need to be better understood before drawing generalities on the biodiversity‐disease relationship.
CircABCC1 promotes the development of glioma by sponging miR‐591 and modulating high‐mobility group A2
Authors: Wang Lei , Tan Ying , Chen Jun , Zhu Ziyu , Zhu Yuting , Sun Qiang , Dong Hao , Ai Chunqi , He Guohou , Liu Yong 
Published: January 2022

AbstractCircABCC1 plays an oncogenic role in diverse malignancies. In this study, we investigated its involvement in glioma. The expression of circABCC1 and miR‐591 was detected in glioma tissues and cell lines. Gain‐ and loss‐of‐function assays were performed to determine the biological effects of circABCC1, miR‐591, and high‐mobility group A2 (HMGA2) in glioma cells. The circABCC1‐mediated competitive endogenous RNA (ceRNA) regulatory mechanism was explored by bioinformatics and the luciferase reporter assay combined with the biotinylated RNA pulldown assay. The effect of circABCC1 on the tumorigenicity of glioma in vivo was detected by constructing xenografts in nude mice. CircABCC1 was highly expressed, and miR‐591 was downregulated in glioma tissues and cells. Suppression of circABCC1 repressed the malignant behaviors of glioma cells and tumor growth. Through the ceRNA mechanism, circABCC1 interacts with miR‐591 to regulate the expression of HMGA2. CircABCC1 functions as an oncogene to promote the progression of glioma via the regulation of miR‐591/HMGA2 signaling. In summary, as revealed by our study, circABCC1 promotes the expression of HMGA2 via sponging of miR‐591, thus affecting glioma progression as an important onco‐circRNA.
Calcium supplementation for the prevention of hypertensive disorders of pregnancy: current evidence and programmatic considerations
Authors: Gomes Filomena , Ashorn Per , Askari Sufia , Belizan Jose M. , Boy Erick , Cormick Gabriela , Dickin Katherine L. , Driller‐Colangelo Amalia R. , Fawzi Wafaie , Hofmeyr G. Justus , Humphrey Jean , Khadilkar Anuradha , Mandlik Rubina , Neufeld Lynnette M. , Palacios Cristina , Roth Daniel E. , Shlisky Julie , Sudfeld Christopher R. , Weaver Connie , Bourassa Megan W. 
Published: January 2022

AbstractMost low‐ and middle‐income countries present suboptimal intakes of calcium during pregnancy and high rates of mortality due to maternal hypertensive disorders. Calcium supplementation during pregnancy is known to reduce the risk of these disorders and associated complications, including preeclampsia, maternal morbidity, and preterm birth, and is, therefore, a recommended intervention for pregnant women in populations with low dietary calcium intake (e.g., where ≥25% of individuals in the population have intakes less than 800 mg calcium/day). However, this intervention is not widely implemented in part due to cost and logistical issues related to the large dose and burdensome dosing schedule (three to four 500‐mg doses/day). WHO recommends 1.5–2 g/day but limited evidence suggests that less than 1 g/day may be sufficient and ongoing trials with low‐dose calcium supplementation (500 mg/day) may point a path toward simplifying supplementation regimens. Calcium carbonate is likely to be the most cost‐effective choice, and it is not necessary to counsel women to take calcium supplements separately from iron‐containing supplements. In populations at highest risk for preeclampsia, a combination of calcium supplementation and food‐based approaches, such as food fortification with calcium, may be required to improve calcium intakes before pregnancy and in early gestation.