bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsa_circ_0005529 promotesZEB1expression by regulating miR‐873‐5p and enhancing proliferation, invasion, and migration in gastric cancer cell lines
Authors: Su Xiaobao , Zhou Rui , Wu Yingjie , Zhang Yixin , Yu Yu , Lu Mengxiao 
Published: October 2022

AbstractBackgroundGastric cancer is a relatively common tumor. As circular RNAs (circRNAs) are documented to modulate proliferation and metastasis in various cancers, we evaluated the functions of circRNAs, in particular, hsa_circ_0005529, in gastric cancer cells.MethodsLevels of hsa_circ_0005529 and miR‐873‐5p were examined by qRT‐PCR, and the presence of hsa_circ_0005529 was confirmed by RNase R treatment. CCK‐8, wound‐healing, and Transwell assays were used to assess proliferation, migration, and invasion, respectively, while Western blotting was used to determine levels of zinc finger E‐box‐binding homeobox 1 (ZEB1) and dual‐luciferase reporter assays to examine relationships between hsa_circ_0005529 and miR‐873‐5p.Resultshsa_circ_0005529 was strongly expressed in gastric cancer where it stimulated tumorigenic behavior. Furthermore, hsa_circ_0005529 was shown to promoteZEB1expression by sponging miR‐873‐5p, an inhibitor ofZEB1expression.ConclusionOur research showed that hsa_circ_0005529 promoted tumorigenic behavior in gastric cancer cells by adsorbing miR‐873‐5p to modulateZEB1levels. This suggests that hsa_circ_0005529 may be useful as a biomarker and target for diagnosing and treating gastric cancer.
Neonatal screening for isovaleric aciduria: Reducing the increasingly high false‐positive rate in Germany
Authors: Murko Simona , Aseman Asra Dadkhah , Reinhardt Friederike , Gramer Gwendolyn , Okun Jürgen Günther , Mütze Ulrike , Santer René 
Published: October 2022

AbstractNewborn screening (NBS) for isovaleric acidemia (IVA) is performed by flow injection tandem mass spectrometry quantifying C5 carnitines (C5). Isovalerylcarnitine, however, is isomeric with pivaloylcarnitine which can be present in blood due to maternal use of pivaloylester‐containing antibiotics, available in Germany since late 2016. During a 36‐month period (January 19–December 21), all newborns screened in Hamburg with a C5 above cutoff (NeoGram®: 0.50 μmol/L or Neobase®2: 0.45 μmol/L) were included in the study. As a second‐tier test, a simple ultra performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS) method was developed to differentiate the C5 isomers pivaloyl‐, 2‐methylbutyryl‐, isovaleryl‐, and valerylcarnitine. Out of 156 772 newborns tested, one turned out to have genetically proven IVA while 99 were false positive (C5: 0.5–8.2 μmol/L) due to the presence of pivaloylcarnitine. These cases have increased year by year and show local clusters. Retrospective analysis of another 39 cases from 287 206 neonates tested at the NBS center in Heidelberg with C5 elevation (0.9–10.6 μmol/L) but clinical and biochemical exclusion of IVA yielded evidence of pivaloylcarnitine in all cases. Inclusion of a second‐tier test into NBS significantly reduces the high and increasing false‐positive rate of IVA screening. This avoids further diagnostic steps, prevents unnecessary stress and anxiety of parents in a remarkably high number of cases. If Hamburg data of 2021 are extrapolated to all of Germany, one can assume around 800 (1‰) false‐positive cases in comparison to an average of two classic IVA cases per year. Unless licensing of pivaloylester‐containing drugs for use during pregnancy is reconsidered, a second‐tier test for C5 determination is indispensable.
Foaming properties are improved by interactions between brewer's spent grain proteins and carrageenans in aqueous solution
Authors: Proaño Janina Lissette , Pérez Adrián Alejandro , Drago Silvina Rosa 
Published: October 2022

AbstractBACKGROUNDFoaming properties and macromolecular interactions in solution among brewer's spent grain proteins (CP) and iota and lambda carrageenans (i‐CG and l‐CG, respectively) as a function of aqueous medium pH (2–6) and protein‐polysaccharide ratio, RCP:CG (1:1, 2:1 and 4:1), were studied. At these conditions, the CP colloidal stability was favored by the formation of soluble electrostatic complexes with CG. Fluorescence (intrinsic and extrinsic) spectroscopy and dynamic light scattering techniques, including particle size and ζ‐potential analysis, were applied to know the phase behavior of the biopolymer systems. The bubbling method was used to produce foams, and the foam expansion (%) and half‐life time (t1/2) were determined.RESULTSBoth CG promoted an increased Trp fluorescence emission depending on the pH, suggesting conformational changes in CP. The CG in mixed systems produced a significant decrease in the extrinsic fluorescence intensity, mainly at low pH values, highlighting a reduction in CP surface hydrophobicity. At the examined pH range, the ζ‐potential values for mixed‐systems were negative, and their magnitudes were intermediate between CP and CG, revealing the associative electrostatic nature of biopolymer interactions, which were dependent on the RCP:CG. The particle size analysis confirmed the formation of soluble electrostatic complexes in solution. Finally, using i‐CG at pH 2 or 3 and 2:1 RCP:CG, the best foaming properties for mixed systems were observed.CONCLUSIONThe formation of electrostatic complexes with a compact assembly among biopolymers, high negative net charge, and colloidal stability convert the CP‐CG mixed solutions into promising biopolymer systems for food foams production. © 2022 Society of Chemical Industry.
Supplement of Caulerpa lentillifera polysaccharide by pre‐prandial gavage and free feeding demonstrates differences to prevent obesity and gut microbiota disturbance in mice
Authors: You Ying , Song Haoran , Wang Linlin , Liu Zhengqi , Guo Xiaoming , Ai Chunqing , Song Shuang , Zhu Beiwei 
Published: October 2022

AbstractBACKGROUNDCaulerpa lentillifera has received extensive attention regarding expansion of its farming and increasing consumption. In our previous study, the structure of C. lentillifera polysaccharide (CLP) was elucidated. However, little information is available about its health effects. In this study, the anti‐obesity effect of CLP was investigated by using a high‐fat diet‐induced obese mice model with two different supplementation methods.RESULTSIn vitro simulated digestion results showed that CLP significantly decreased the lipid digestibility and induced the lipid droplets aggregation in the intestinal stage to inhibit the absorption of lipids. As revealed by 16S ribosomal RNA sequencing and non‐targeted metabolomics, supplement of CLP by both pre‐prandial gavage and free feeding patterns effectively prevented mice obesity via ameliorating intestinal flora disturbance and regulating bile acids circulation metabolism. Of note was that CLP administration had no effect on short‐chain fatty acids production, suggesting the anti‐obesity effect was uncorrelated with their production. Moreover, pre‐prandial administration of CLP had a better anti‐obesity effect in lowering body weight and serum lipid levels, but the free feeding resulted in a higher α‐diversity of gut microbiota.CONCLUSIONThe findings of this study indicate that CLP could be a potential anti‐obesity nutraceutical and that pre‐prandial supplement of CLP may be a better intake method to exhibit its hypolipidemic effect. © 2022 Society of Chemical Industry.
The Use of Dissemination and Implementation to Improve Multimodal Analgesia in Head and Neck Surgery
Authors: Shnayder Yelizaveta , Baumanis Maraya M. , Brown Adam , Reese Adam , Bur Andrés M. , Kakarala Kiran , Sykes Kevin J. 
Published: October 2022

ObjectivesTo optimize the delivery of multimodal analgesia to patients undergoing major head and neck oncologic surgeries.MethodsPilot study included patients enrolled to receive either scheduled acetaminophen and as‐needed opioids (control group) or scheduled acetaminophen, gabapentin, ketorolac, and as‐needed opioids (experimental group). RCT, a hybrid type 1 effectiveness‐implementation pragmatic trial, was designed to test the effectiveness of the intervention. Arm A received scheduled acetaminophen and as‐needed opioids. Arm B received scheduled gabapentin, ketorolac, a regional nerve block at the free tissue donor site, scheduled acetaminophen and as‐needed opioids.ResultsPilot: Thirty‐one patients undergoing major head and neck surgery were enrolled. Mean MMEs administered in control group (n = 15) was 251.60 mg (SD = 224.57 mg); mean MMEs in Experimental group (n = 16) was 195.78 mg (SD = 131.08 mg), p = 0.401. LOS was 8.0 days in control versus 7.0 days in experimental group (p = 0.054).RCT: Interim analysis for safety and futility was planned during trial's design after 30 patients (n = 14 Arm A, and n = 16 Arm B). Mean MMEs administered were 135.1 mg in Arm A, (SD = 86.0 mg) versus mean MME of 51.3 mg in Arm B (SD = 43.3 mg, (p < 0.05)). Given clear superiority results, the trial was prematurely terminated. Functional pain scores, LOS, and complications were similar between the arms (p > 0.05). Variability of mean MME was compared before and after implementation of the management protocols: SD in RCT#1 was 181.46 mg versus 124.6 mg in RCT#2.ConclusionMultimodal analgesia significantly reduced the need for opioids in patients undergoing major head and neck surgery.Level of Evidence1, Randomized Clinical Trial Laryngoscope, 133:S1–S11, 2023
Validation of a three‐dimensional printed pediatric middle ear model for endoscopic surgery training
Authors: Stramiello Joshua Anthony , Wong Stephanie J. , Good Raquel , Tor Alice , Ryan Justin , Carvalho Daniela 
Published: October 2022

AbstractObjectiveThe purpose of this study is to assess the anatomical appropriateness of a three‐dimensional (3D) printed pediatric middle ear model with a replaceable middle ear unit as an endoscopic ear surgery (EES) simulator.MethodsSingle‐blinded, prospective, proof‐of‐concept study conducted in a simulation operative suite. A simulator was developed through segmentation of source images and multi‐material 3D printing. Subjects were asked to point to seven anatomical sites before and after a short anatomy presentation of a human middle ear photograph. They also filled out a survey about the feasibility of the model. Outcome variables included survey scores, pre‐anatomy lesson (PreAL) and post‐anatomy lesson (PostAL) quiz scores.ResultsThere were 24 participants (19 residents, 1 fellow, and 4 attendings), none with self‐reported proficiency in EES. The PreAL mean score was 4.42 and PostAL quiz mean score was 5.32 (average improvement of 43% [CI = 17%–70%]; p = .003). The higher the level of training, the higher the PreAL scores (0.55 points per year of training; p = .004). The subspecialty (otology, other, in‐training) was also associated with the PreAL scores (p = .004). Total survey score means were 22.8 (out of 30).ConclusionThe results of our study suggest that our model has adequate anatomical high fidelity to mimic a real, pediatric temporal bone for EES. As 3D printing technologies continue to advance, the quality of ear models has the potential to provide improved surgical training for pediatric EES.Level of Evidence4
A Porous Gelatin Methacrylate‐Based Material for 3D Cell‐Laden Constructs
Authors: Bova Lorenzo , Maggiotto Federico , Micheli Sara , Giomo Monica , Sgarbossa Paolo , Gagliano Onelia , Falcone Dario , Cimetta Elisa 
Published: October 2022

Abstract3D constructs are fundamental in tissue engineering and cancer modeling, generating a demand for tailored materials creating a suitable cell culture microenvironment and amenable to be bioprinted. Gelatin methacrylate (GelMA) is a well‐known functionalized natural polymer with good printability and binding motifs allowing cell adhesion; however, its tight micropores induce encapsulated cells to retain a non‐physiological spherical shape. To overcome this problem, blended GelMa is here blended with Pluronic F‐127 (PLU) to modify the hydrogel internal porosity by inducing the formation of larger mesoscale pores. The change in porosity also leads to increased swelling and a slight decrease in Young's modulus. All blends form stable hydrogels both when cast in annular molds and bioprinted in complex structures. Embedded cells maintain high viability, and while Neuroblastoma cancer cells typically aggregate inside the mesoscale pores, Mesenchymal Stem Cells stretch in all three dimensions, forming cell–cell and cell–ECM interactions. The results of this work prove that the combination of tailored porous materials with bioprinting techniques enables to control both the micro and macro architecture of cell‐laden constructs, a fundamental aspect for the development of clinically relevant in vitro constructs.
Seq2Morph: A deep learning deformable image registration algorithm for longitudinal imaging studies and adaptive radiotherapy
Authors: Lee Donghoon , Alam Sadegh , Jiang Jue , Cervino Laura , Hu Yu‐Chi , Zhang Pengpeng 
Published: October 2022

AbstractPurposeTo simultaneously register all the longitudinal images acquired in a radiotherapy course for analyzing patients’ anatomy changes for adaptive radiotherapy (ART).MethodsTo address the unique needs of ART, we designed Seq2Morph, a novel deep learning‐based deformable image registration (DIR) network. Seq2Morph was built upon VoxelMorph which is a general‐purpose framework for learning‐based image registration. The major upgrades are (1) expansion of inputs to all weekly cone‐beam computed tomography (CBCTs) acquired for monitoring treatment responses throughout a radiotherapy course, for registration to their planning CT; (2) incorporation of 3D convolutional long short‐term memory between the encoder and decoder of VoxelMorph, to parse the temporal patterns of anatomical changes; and (3) addition of bidirectional pathways to calculate and minimize inverse consistency errors (ICEs). Longitudinal image sets from 50 patients, including a planning CT and 6 weekly CBCTs per patient, were utilized for network training and cross‐validation. The outputs were deformation vector fields for all the registration pairs. The loss function was composed of a normalized cross‐correlation for image intensity similarity, a DICE for contour similarity, an ICE, and a deformation regularization term. For performance evaluation, DICE and Hausdorff distance (HD) for the manual versus predicted contours of tumor and esophagus on weekly basis were quantified and further compared with other state‐of‐the‐art algorithms, including conventional VoxelMorph and large deformation diffeomorphic metric mapping (LDDMM).ResultsVisualization of the hidden states of Seq2Morph revealed distinct spatiotemporal anatomy change patterns. Quantitatively, Seq2Morph performed similarly to LDDMM, but significantly outperformed VoxelMorph as measured by GTV DICE: (0.799±0.078, 0.798±0.081, and 0.773±0.078), and 50% HD (mm): (0.80±0.57, 0.88±0.66, and 0.95±0.60). The per‐patient inference of Seq2Morph took 22 s, much less than LDDMM (∼30 min).ConclusionsSeq2Morph can provide accurate and fast DIR for longitudinal image studies by exploiting spatial‐temporal patterns. It closely matches the clinical workflow and has the potential to serve both online and offline ART.
Novel magnetic embolic MRI imageable particles with anticancer drug release for transcatheter arterial embolization and magnetic ablation
Authors: Bekaroğlu Maide Gökçe , Nurili Fuad , Caymaz İsmail , Baş Ahmet , İşçi Sevim 
Published: October 2022

AbstractThe aim of the study is to prepare embolic hydroxyl ethyl cellulose (HEC)–polyvinyl prolidone (PVP)–magnetic particles suitable for transcatheter arterial chemoembolization (TACE) procedures, drug delivery, and magnetic hyperthermia. Two different sizes (microsized and nanosized) of iron oxide particles were used to prepare the embolic particles to investigate the embolization and drug delivery properties. Iron oxides were linked with PVP via bridging flocculation process, then outermost layer of the linked particles was coated with HEC in order to load drugs to particles and reach size requirements for a successful TACE procedure. Size of each particle was calibrated to the range that allows easy injections through microcatheters (40–500 μm). The results showed that the size of the final embolic particles reached around 70 μm with 82 W/g specific absorption rate (SAR) values for nano‐iron oxide particles and 45 μm with 77 W/g SAR values for micro‐iron oxide particles, which are quite suitable for TACE applications. Furthermore, an anticancer drug doxorubicin (DOX) was successfully loaded onto these particles in order to achieve localized chemotherapy at the tumor site. Particles produced in this study, loaded DOX successfully and prolonged drug release time, performed similarly to pure DOX at higher concentration treatments against human breast cancer cell lines, were heatable under applied alternating magnetic fields. In addition, in vivo embolization studies performed using a rabbit renal embolization model, indicated that these particles were easily delivered through microcatheters and were able to embolize the target.
Promoting social innovation amidst organizational complexity: Case study evidence from the German Red Cross
Authors: Burfeindt René , Schubert Peter 
Published: October 2022

AbstractSocial innovation is concerned with the creation and implementation of new solutions to social problems. Although research commonly frames social innovation as the domain of small, entrepreneurial organizations, an increasing number of large and well‐established nonprofit organizations have started actively launching their own innovation initiatives. Using a case study of social innovation ventures within the German Red Cross (GRC), this study identifies organizational hurdles and viable management strategies targeting the promotion of social innovation within particularly complex organizations. Based on our results, we develop a conceptual framework highlighting that promoting social innovation in established organizations requires simultaneous attention to multiple dimensions of leadership and governance. Our study thereby offers a blueprint for management strategies that can guide nonprofit leaders in their quest to promote social innovation from within their organization.
Outcomes of family‐centred auditory and tactile stimulation implementation on traumatic brain injured patients
Authors: Ahmed Fatma Refaat , Attia Amal Kadry , Mansour Hamada , Megahed Mohamed 
Published: October 2022

AbstractAimTo determine the outcomes of Family‐centred Auditory and Tactile Stimulation Implementation on Traumatic Brain Injured Patients in Egypt.BackgroundFamily engagement in the care of their relatives in the Intensive care units is limited due to patients' life‐threatening conditions, in addition to the use of high technology in these settings. Auditory and tactile sensory stimulations are among the diverse sensory stimulations that have received more attention in brain injured patients than other senses as being considered safe, and effective measures.DesignA Quasi‐experimental design was used to test the hypotheses of this study.MethodsA convenience sample of 60 adult patients suffering from Traumatic Brain Injury and admitted to the intensive care units of two University Hospitals in Egypt was included in the study. Patients were assigned into two equal groups: control and study groups (30 patients each). The auditory and tactile stimulations were provided by trained family members, once daily for 2 weeks for the study group. Whereas routine communication was provided by the family of traumatic brain injured patients in the ICU for the control group. Two tools were used for data collection; tool one, the “Glasgow Coma Scale” to assess patient's level of consciousness, and tool two the “Physiological Adverse Events Assessment” to monitor patients for the occurrence of physiological adverse events. Data collection: January to October 2019.ResultsThe implementation of an organized auditory and tactile stimulation by trained family members is associated with highly statistically significant positive effects . Patients in the study group showed a higher mean of consciousness, lower incidence rate of physiological adverse events, and a lower mean duration of ICU stay.ConclusionsImplementation of an organized auditory and tactile stimulation by trained family members enhanced the consciousness level of comatose Traumatic Brain Injured patients, decreased the occurrence of physiological adverse events, and ICU length of stay. Thus, it is recommended for use in the daily routine nursing care of comatose Traumatic Brain Injured patients.Relevance to Clinical PracticeThis study gives a deeper understanding of how family engagement in the care of their critically ill relative enhances their recovery and improve their level of consciousness.
Validation of the psychometric properties of the Self‐Directed Learning Readiness Scale
Authors: Chen Shiah‐Lian , Fan Jun‐Yu 
Published: October 2022

AbstractAimThe aim of the study was to evaluate the psychometric properties of the Chinese version of the Self‐Directed Learning Readiness Scale.DesignA cross‐sectional survey was designed.MethodsA convenience sample of 659 participants was recruited. Exploratory and confirmatory factor analyses were used to evaluate the factorial structure. In addition, the concurrent validity was assessed using the Critical Thinking Disposition Inventory.ResultsThe exploratory factor analysis revealed that the scale was a 6‐factor structure, named love of learning, active learning, effective learning, independent learning, learning motivation and creative learning, which explained 53.30% of the total variance. The findings of the confirmatory factor analysis showed that a 37‐item six first‐order model produced the best‐fit statistics. Internal consistency for the scale was satisfactory, ranging from 0.71 to 0.88. Concurrent and predictive validities also reached significant levels. The utility of the instrument was suggested.
The effectiveness of a tailored programme to promote reproductive‐health‐promoting behaviour in young women based on the Precaution Adoption Process Model: A randomized controlled trial
Authors: Cho Inhae , Park Young‐Joo 
Published: October 2022

AbstractAimThe study aimed to evaluate a tailored reproductive health promotion programme based on the Precaution Adoption Process Model (PAPM) for young adults.DesignThis was a two‐group, non‐blinded randomized controlled trial.MethodsThis study was performed with women aged 18–25 who were not performing reproductive health‐related behaviour from September 2018 to November 2018 in Seoul, South Korea. The participants were stratified by stage of PAPM (1, 2 or 3) and were randomly allocated to the intervention group (n = 30) and the control group (n = 30). Women in the intervention group were given the programme, which was customized according to the stage of PAPM over 8 weeks, such as lectures, giving information, discussions, practical training, Q&A, case experience and counselling. The control group received educational material. The primary outcomes were reproductive‐health‐promoting behaviour and rate of contraception use.ResultsReproductive‐health‐promoting behaviour was significantly higher in the intervention group than the control group post‐intervention, as were reproductive health beliefs, motivation and self‐efficacy.