bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancement strategies for mesenchymal stem cells and related therapies
Authors: Alagesan Senthilkumar , Brady Jack , Byrnes Declan , Fandiño Juan , Masterson Claire , McCarthy Sean , Laffey John , O’Toole Daniel 
Published: February 2022

AbstractCell therapy, particularly mesenchymal stem/stromal (MSC) therapy, has been investigated for a wide variety of disease indications, particularly those with inflammatory pathologies. However, recently it has become evident that the MSC is far from a panacea. In this review we will look at current and future strategies that might overcome limitations in efficacy. Many of these take their inspiration from stem cell niche and the mechanism of MSC action in response to the injury microenvironment, or from previous gene therapy work which can now benefit from the added longevity and targeting ability of a live cell vector. We will also explore the nascent field of extracellular vesicle therapy and how we are already seeing enhancement protocols for this exciting new drug. These enhanced MSCs will lead the way in more difficult to treat diseases and restore potency where donors or manufacturing practicalities lead to diminished MSC effect.
Modeling oxidative injury response in human kidney organoids
Authors: Przepiorski Aneta , Vanichapol Thitinee , Espiritu Eugenel B. , Crunk Amanda E. , Parasky Emily , McDaniels Michael D. , Emlet Dave R. , Salisbury Ryan , Happ Cassandra L. , Vernetti Lawrence A. , MacDonald Matthew L. , Kellum John A. , Kleyman Thomas R. , Baty Catherine J. , Davidson Alan J. , Hukriede Neil A. 
Published: February 2022

Abstract
Background
Hemolysis occurs in many injury settings and can trigger disease processes. In the kidney, extracellular hemoglobin can induce damage via several mechanisms. These include oxidative stress, mitochondrial dysfunction, and inflammation, which promote fibrosis and chronic kidney disease. Understanding the pathophysiology of these injury pathways offers opportunities to develop new therapeutic strategies.

Methods
To model hemolysis-induced kidney injury, human kidney organoids were treated with hemin, an iron-containing porphyrin, that generates reactive oxygen species. In addition, we developed an induced pluripotent stem cell line expressing the biosensor, CytochromeC-GFP (CytoC-GFP), which provides a real-time readout of mitochondrial morphology, health, and early apoptotic events.

Results
We found that hemin-treated kidney organoids show oxidative damage, increased expression of injury markers, impaired functionality of organic anion and cation transport and undergo fibrosis. Injury could be detected in live CytoC-GFP organoids by cytoplasmic localization of fluorescence. Finally, we show that 4-(phenylthio)butanoic acid, an HDAC inhibitor with anti-fibrotic effects in vivo, reduces hemin-induced human kidney organoid fibrosis.

Conclusion
This work establishes a hemin-induced model of kidney organoid injury. This platform provides a new tool to study the injury and repair response pathways in human kidney tissue and will assist in the development of new therapeutics.
Porcine reproductive and respiratory virus 2 infection of the fetus results in multi-organ cell cycle suppression
Authors: Mulligan Margaret K. , Kleiman Jocelyn E. , Caldemeyer Andrew C. , Harding John C. S. , Pasternak J. Alex 
Published: February 2022

AbstractPorcine reproductive and respiratory syndrome virus (PRRSV) infection during late gestation negatively affects fetal development. The objective of this study was to identify the fetal organs most severely impacted following infection, and evaluate the relationship between this response and fetal phenotypes. RNA was extracted from fetal heart, liver, lung, thymus, kidney, spleen, and loin muscle, collected following late gestation viral challenge of pregnant gilts. Initially, gene expression for three cell cycle promoters (CDK1, CDK2, CDK4) and one inhibitor (CDKN1A) were evaluated in biologically extreme phenotypic subsets including gestational age-matched controls (CON), uninfected (UNIF), high-viral load viable (HV-VIA), and high-viral load meconium-stained (HV-MEC) fetuses. There were no differences between CON and UNIF groups for any gene, indicating no impact of maternal infection alone. Relative to CON, high-viral load (HV-VIA, HV-MEC) fetuses showed significant downregulation of at least one CDK gene in all tissues except liver, while CDKN1A was upregulated in all tissues except muscle, with the heart and kidney most severely impacted. Subsequent evaluation of additional genes known to be upregulated following activation of P53 or TGFb/SMAD signaling cascades indicated neither pathway was responsible for the observed increase in CDKN1A. Finally, analysis of heart and kidney from a larger unselected population of infected fetuses from the same animal study showed that serum thyroxin and viral load were highly correlated with the expression of CDKN1A in both tissues. Collectively these results demonstrate the widespread suppression in cell division across all tissues in PRRSV infected fetuses and indicate a non-canonical regulatory mechanism.
A remark on Secelean–Wardowski’s fixed point theorems
Authors: Saejung Satit , Ardsalee Pinya 
Published: February 2022

AbstractIn this paper we give a simple proof of three fixed point theorems of Secelean and Wardowski by using the fixed point result of Jachymski et al. Our result is established with weaker assumptions than the three theorems. Furthermore, the recent result of Secelean et al. in the setting of a complete metric space can be also deduced by our theorem.
Effect of market-driven strategies on the competitive growth of SMEs in Lesotho
Authors: Amadasun Donald O. E. , Mutezo Ashley. T. 
Published: February 2022

AbstractThis paper established some market-driven strategies that influence the competitive growth of small and medium-sized enterprises in Lesotho. The paper identified the following factors; market orientation, competitive intensity, and technological dynamics as variables that influence SMEs’ competitive growth. The investigation shows that these critical factors of market-driven strategies are significant market facets in the enterprise that could capacitate SME entrepreneurs and managers to attain competitive growth. The results indicated that the factors used to measure market-driven strategies influence SMEs competitive growth. More specifically, the independent variables of market orientation, competitive intensity and technological dynamics are seen as key tenets of market-driven strategies that influence small and medium-sized enterprises’ competitive growth. From the analyses, this paper recommended that market-driven strategic constructs of market orientation, competitive intensity and technological dynamics are inimitable and tangible significant resources if harnessed in the enterprises, could serve as critical operational factors that influence SMEs competitive growth in Lesotho.
Early life inter-kingdom interactions shape the immunological environment of the airways
Authors: Pattaroni Céline , Macowan Matthew , Chatzis Roxanne , Daunt Carmel , Custovic Adnan , Shields Michael D. , Power Ultan F. , Grigg Jonathan , Roberts Graham , Ghazal Peter , Schwarze Jürgen , Gore Mindy , Turner Steve , Bush Andrew , Saglani Sejal , Lloyd Clare M. , Marsland Benjamin J. 
Published: February 2022

AbstractBackgroundThere is increasing evidence that the airway microbiome plays a key role in the establishment of respiratory health by interacting with the developing immune system early in life. While it has become clear that bacteria are involved in this process, there is a knowledge gap concerning the role of fungi. Moreover, the inter-kingdom interactions that influence immune development remain unknown. In this prospective exploratory human study, we aimed to determine early post-natal microbial and immunological features of the upper airways in 121 healthy newborns.ResultsWe found that the oropharynx and nasal cavity represent distinct ecological niches for bacteria and fungi. Breastfeeding correlated with changes in microbiota composition of oropharyngeal samples with the greatest impact upon the relative abundance ofStreptococcusspecies andCandida. Host transcriptome profiling revealed that genes with the highest expression variation were immunological in nature. Multi-omics factor analysis of host and microbial data revealed unique co-variation patterns.ConclusionThese data provide evidence of a diverse multi-kingdom microbiota linked with local immunological characteristics in the first week of life that could represent distinct trajectories for future respiratory health.Trial registrationNHS Health Research Authority, IRAS ID 199053. Registered 5 Oct 2016.https://www.hra.nhs.uk/planning-and-improving-research/application-summaries/research-summaries/breathing-together/
From efficacy to effectiveness: child and adolescent eating disorder treatments in the real world (part 1)—treatment course and outcomes
Authors: Simic Mima , Stewart Catherine S. , Konstantellou Anna , Hodsoll John , Eisler Ivan , Baudinet Julian 
Published: February 2022

AbstractBackgroundFindings from randomised control trials inform the development of evidence-based eating disorder (ED) practice guidelines internationally. Only recently are data beginning to emerge regarding how these treatments perform outside of research settings. This study aimed to evaluate treatment pathways and outcomes for a specialist child and adolescent ED service across a five-year period.MethodsAll consecutive referrals between August 2009 and January 2014 seen at the Maudsley Centre for Child and Adolescent Eating Disorders in London were included. Data are reported on for all young people who were offered treatment (N = 357).ResultsMost young people referred to the service were diagnosed with anorexia nervosa (AN)/Atypical AN (81%). Treatment for AN/Atypical AN (median 11 months) was predominantly ED focused family therapy (99%). Treatment for bulimia nervosa (BN)/Atypical BN (median seven months) was most commonly a combination of cognitive behavioural therapy and ED focused family therapy (87%). At discharge, 77% of the AN/Atypical AN group had a good or intermediate outcome and 59% of the BN/Atypical BN group reported no or fewer than weekly bulimic episodes. 27% of the AN/Atypical AN group had enhanced treatment with either day- and/or inpatient admissions (AIM group). The %mBMI at 3 months of treatment was strongest predictor of the need for treatment enhancement and more modestly EDE-Q and age at assessment. The AIM group at assessment had significantly lower weight, and higher ED and comorbid symptomatology and went on to have significantly longer treatment (16 vs. 10 months). At discharge, this group had significantly fewer good and more poor outcomes on the Morgan Russell criteria, but similar outcomes regarding ED and comorbid symptoms and quality of life. When analysis was adjusted for %mBMI at assessment, 1 and 3 months of treatment, differences in Morgan Russell outcomes and %mBMI were small and compatible with no difference in outcome by treatment group.ConclusionsThis study shows that outcomes in routine clinical practice in a specialist community-based service compare well to those reported in research trials. The finding from research trials that early weight gain is associated with improved outcomes was also replicated in this study. Enhancing outpatient treatment with day treatment and/or inpatient care is associated with favourable outcome for most of the young people, although a longer duration of treatment is required.
The message of oldhamites from enstatite chondrites
Authors: Hammouda Tahar , Boyet Maud , Frossard Paul , Cartier Camille 
Published: February 2022

AbstractWe have determined rare-earth element (REE) abundances in oldhamites (CaS) from 13 unequilibrated and equilibrated enstatite chondrites (5 EH and 8 EL) and in a few enstatites by in situ, laser ablation ICP-MS. In EH chondrites, oldhamite REE patterns vary from the most primitive petrographic types (EH3) to the most metamorphosed types (EH5). In EH3, CI-normalized REE patterns are convex downward with strong positive Eu and Yb anomalies, whereas EH5 display flat patterns with enrichments reaching about 80 times CI abundances. The positive anomalies of Eu and Yb found in oldhamites of primitive EH chondrites indicate that they represent the condensation of a residual gas fraction, in a manner similar to fine-grained CAIs of carbonaceous chondrites. The early condensate may have been preserved in the matrix of unequilibrated EH. Equilibrated EH oldhamite patterns may result from metamorphic evolution and REE redistribution on the EH parent body. On the contrary, all the oldhamites from EL chondrites (EL3 to EL6) display a single kind of patterns, which is convex upward and is about 100 times enriched relative to CI, with a negative Eu anomaly. In addition, the EL pattern is similar to that of oldhamites from aubrites (enstatite achondrites). The latter observation suggests that oldhamites of all EL metamorphic types (including primitive ones) bear the signature of a magmatic event accompanied by FeS loss as vapor, prior to the assembly of the EL parent body. Given the difficulty of obtaining precise ages on enstatite chondrites, it is not possible to discuss the chronology of the events recorded by the oldhamite REE patterns.
Torrent rainfall-induced large-scale karst limestone slope collapse at Khanh waterfall, Hoa Binh Province, Vietnam
Authors: Do Ngoc Ha , Goto Satoshi , Abe Shinro , Nguyen Kim Thanh , Miyagi Toyohiko , Hayashi Kazunori , Watanabe Osamu 
Published: February 2022

AbstractIn recent years, many landslides have occurred in Vietnam, particularly in the Northern mountainous region during the rainy season from May to October. On the morning of October 12, 2017, the Khanh waterfall landslide in Khanh Village, Hoa Binh Province, Northern Vietnam occurred. The landslide killed eighteen people and destroyed five houses. Topographical and geological surveys were conducted around the area to determine its causes. The rainfall data and flow discharge were also analyzed. The results showed that this collapse was different from some previous ones collapsed due to the erosion at the toe of the slope. Khanh waterfall landslide occurred due to the increasing amount of water in cracks and caves in the limestone layer in the slope. The collapse process was simulated based on Coulomb mixture theory. The numerical simulation results show similarities with the actual collapse process. The results provide indicators for assessing the risk of such limestone waterfall landslides in the future.
My nutrition index: a method for measuring optimal daily nutrient intake
Authors: Busgang Stefanie A. , Malin Ashley J. , Gennings Chris 
Published: February 2022

Abstract
Background
Adequate nutrition is essential for individual and population level health. However, determining adequacy of daily nutrient intake in research studies is often challenging given the unique nutritional needs of individuals. Herein, we examine construct, predictive, criterion, content, and concurrent validity of a dietary analytic tool – My Nutrition Index (MNI) for measuring nutrient intake in relation to personalized daily nutrient intake guidelines. MNI gauges adequacy of an individual’s daily nutrient intake based on his or her unique demographic and lifestyle characteristics. MNI accounts for potential adverse effects of inadequate and excess nutrient consumption.

Methods
MNI, calculated based on 34 nutrients, provides an overall index score ranging from 0 to 100, with higher scores reflecting a more nutritious diet. We calculated MNI scores for 7154 participants ages 18-65 in the National Health and Nutrition Examination Surveys (2007-2014) by using average nutrient intakes from two 24-h dietary recalls. Survey-weighted binary logistic regression models were used to assess associations between MNI scores and obesity, depression, health perceptions, and past or present cardiovascular disease.

Results
Higher MNI scores were associated with lower prevalence of self-reported cardiovascular disease (OR = 0.69, CI: 0.52, 0.92, p = 0.012), depression (OR = 0.76, CI: 0.65, 0.90, p < 0.001), and obesity (OR = 0.92, CI: 0.87, 0.99, p = 0.016), as well as more favorable health perceptions (OR = 1.24, CI: 1.13, 1.37, p < 0.001).

Conclusions
MNI provides an individualized approach for measuring adequacy/sufficiency of daily nutrient intake that can validly be employed to assess relationships between nutrition and health outcomes in research studies.
A preliminary study of the efficacy of the polyherbal preparation Sao Thong Tai for erectile dysfunction among elderly men: a double-blind, randomized controlled trial
Authors: Lin Chakariya , Pattraraachachai Junya , Pawa Kammal K. , Wongyai Surapote 
Published: February 2022

Abstract
Background
The traditional Thai polyherbal formulation “Sao Thong Tai” (STT) contains four medicinal plants, namely Boesenbergia rotunda (L.) Mansf., Sida acuta Burm.f., Dactyloctenium aegyptium (L.) Willd., and Oryza sativa L. and it is considered an aphrodisiac and remedy for the effective treatment of decreased male libido in Thai traditional medicine and it may ameliorate erectile function. This pilot study was carried out to investigate the efficacy and safety of the polyherbal STT for mild to moderate erectile dysfunction in a small group of elderly men in Thailand in a double-blind, randomized controlled trial.

Methods
This research is a preliminary study. Fifty-four elderly males aged 50-69 years who had been diagnosed with mild to moderate ED were randomized into two groups of equal size: an STT group (n=27) and a placebo control group (n=27). The patients received either an 800 mg dose of STT extract or a lactose placebo twice a day for the eight-week treatment period, after which they were followed up for four weeks. Erectile function, sexual activity, self-reported attitudes toward sexual dysfunction, and partner-reported sexual dysfunction were investigated to confirm the efficacy of the polyherbal preparation. The International Index of Erectile Function (IIEF-5) and the Scale for Quality of Sexual Function (QSF) were used for primary outcome assessment.

Results
The IIEF-5 scores of the STT group were significantly increased compared with those of the placebo group (p<0.001). The severity of ED decreased from "mild" or "mild to moderate" to normal in 88.9% of the STT group vs. 0% of the placebo group, and the QSF score increased in the STT group (p<0.001). Sexual activity, self-reported views of sexual dysfunction, and partners’ views of sexual dysfunction showed significant improvement in the STT group, and there was no significant difference in psychosomatic quality of life. No adverse events were observed in either group.

Conclusion
Compared with a placebo, an oral aqueous extract of STT taken daily at a dose of 1,600 mg for eight weeks can effectively treat mild to moderate ED in elderly men. The preparation was also shown to be safe with respect to all parameters assessed. This finding demonstrates that STT can be used for the effective treatment of decreased male libido in Thai traditional medicine and may ameliorate ED.

Trial registration
TCTR/20180126001 033/2560. Registered 16 January 2018, Thai Clinical Trials Registry http://www.thaiclinicaltrials.org/
Household food security during the COVID-19 pandemic in urban and semi-urban areas in Indonesia
Authors: Syafiq Ahmad , Fikawati Sandra , Gemily Syilga Cahya 
Published: February 2022

Abstract
Background
One of the impacts of the COVID-19 pandemic was the weakening of the community's economic condition. The weak economy of the community will have an impact on household food security. This study aims to determine food security in the COVID-19 pandemic situation and the impact of the pandemic on food security in urban and semi-urban areas.

Methods
A cross-sectional study with a total sample of 517 people who live in urban (Jakarta) and semi-urban (Depok) areas. The research data was collected online and purposively through Posyandu cadres who have access to family/community. Food security was measured using HFIAS (Household Food Insecurity Access Scale) method, while impact of COVID-19 pandemic was categorized into two categories: impacted (reduced income and laid off) and less impacted (not reduced income and laid off). Data analysis used the Chi-square test and multiple logistic regression.

Results
There were 65.0% of households with various level of food insecurity during the COVID-19 pandemic. The results of the multivariate analysis showed that family income during the COVID-19 pandemic (AOR = 4.2; CI = 2.7–6.7), the type of impact of the COVID-19 pandemic, i.e., reduced income and stopped working (AOR = 2.6; CI = 1.6–4.1), and the age of the respondent (AOR = 1.7; CI = 1.1–2.5) were significantly related to household food security during the pandemic after being controlled by husband's work status. Households with lower income had 4 times higher risk to experience food insecurity compared to those with higher income. Heavily impacted households (through reduced income and stopped working) had 3 times higher risk to experience food insecurity compared to those who did not. Additionally, we found that households with younger respondent (< 31 years old) had 2 times higher risk to experience food insecurity compared to those older counterparts.

Conclusions
The COVID-19 pandemic impacted household food security in both urban and semi-urban areas through worsening employment status and income condition.
Identification of a novel four-gene diagnostic signature for patients with sepsis by integrating weighted gene co-expression network analysis and support vector machine algorithm
Authors: Li Mingliang , Huang He , Ke Chunlian , Tan Lei , Wu Jiezhong , Xu Shilei , Tu Xusheng 
Published: February 2022

AbstractSepsis is a life-threatening condition in which the immune response is directed towards the host tissues, causing organ failure. Since sepsis does not present with specific symptoms, its diagnosis is often delayed. The lack of diagnostic accuracy results in a non-specific diagnosis, and to date, a standard diagnostic test to detect sepsis in patients remains lacking. Therefore, it is vital to identify sepsis-related diagnostic genes. This study aimed to conduct an integrated analysis to assess the immune scores of samples from patients diagnosed with sepsis and normal samples, followed by weighted gene co-expression network analysis (WGCNA) to identify immune infiltration-related genes and potential transcriptome markers in sepsis. Furthermore, gene regulatory networks were established to screen diagnostic markers for sepsis based on the protein-protein interaction networks involving these immune infiltration-related genes. Moreover, we integrated WGCNA with the support vector machine (SVM) algorithm to build a diagnostic model for sepsis. Results showed that the immune score was significantly lower in the samples from patients with sepsis than in normal samples. A total of 328 and 333 genes were positively and negatively correlated with the immune score, respectively. Using the MCODE plugin in Cytoscape, we identified four modules, and through functional annotation, we found that these modules were related to the immune response. Gene Ontology functional enrichment analysis showed that the identified genes were associated with functions such as neutrophil degranulation, neutrophil activation in the immune response, neutrophil activation, and neutrophil-mediated immunity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed the enrichment of pathways such as primary immunodeficiency, Th1- and Th2-cell differentiation, T-cell receptor signaling pathway, and natural killer cell-mediated cytotoxicity. Finally, we identified a four-gene signature, containing the hub genesLCK,CCL5,ITGAM, andMMP9, and established a model that could be used to diagnose patients with sepsis.