bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Internal Monitoring on Compliance with Effluent Limits
Authors: Brandes Julia , Earnhart Dietrich 
Published: April 2022

Using unique data on chemical manufacturing facilities regulated under the Clean Water Act (CWA), this paper analyzes the effect of one particular environmental management practice — internal monitoring — on regulated facilities’ compliance with wastewater discharge limits. To deepen our understanding of the role played by internal monitoring, we extend our analysis by exploring the interaction between internal monitoring and self-audits, which should share a similar purpose to internal monitoring by providing systematic information to regulated facilities on their pollution abatement efforts. Baseline empirical results robustly demonstrate that increases in the extent of internal monitoring significantly improve facilities’ environmental compliance. The extended empirical results offer moderate evidence that audits complement internal monitoring (and vice versa): as facilities audit more frequently, the productive effect of increased internal monitoring grows.
INHIBITORY EFFECT OF ULIN WOOD LIQUID SMOKE AND GOGO RICE ENDOPHYTIC FUNGI AGAINST PATHOGEN Pyricularia oryzae
Authors: Imaningsih Witiyasti , Mariana  , Junaidi Ahmad Budi , Adventaria Della 
Published: April 2022

Diseases in rice plants (paddy) caused by microorganisms such as Pyricularia oryzae lead to a decrease in rice production. Therefore, it is essential to find out biological agents for protecting paddy and plants in general, against plant diseases. Liquid smoke and endophytic fungi have been known as biological agents to enhance the protection of plants against disease. The purpose of this study was to determine the ability of liquid smoke, endophytic fungi and the concentrations combinations to suppress the growth of P. oryzae. The results showed that liquid smoke concentrations of 0.17% to 1.75% and endophytic fungi filtrate of 2% to 10% showed significant ability against pathogen P. oryzae. However, the combination of liquid smoke and endophytic fungi filtrate at selected concentrations (0.17% liquid smoke combined with 2% endophytic fungi filtrate and 0.34% liquid smoke combined with 2% endophytic fungi filtrate) showed no significant inhibition percentage against P. oryzae compared to control. In conclusion, this study showed that the respective applications of liquid smoke and endophytic fungi filtrate inhibit the growth of P. oryzae.

Keywords: endophytic fungi, inhibition ability, liquid smoke, P. oryzae
Ceticismo e dogmatismo em Pascal
Authors: Oliva Luís César Guimarães 
Published: April 2022

O objetivo deste artigo é comparar a maneira como Pascal trata das relações entre ceticismo e dogmatismo em duas de suas principais obras, os Pensamentos e Conversa com o Senhor de Sacy, as quais, embora não conflitantes, trazem uma relevante mudança de pontos de vista. A Conversa aborda o debate ceticismo/dogmatismo diretamente pelo ângulo moral, de modo que os riscos morais (e as paixões correspondentes de orgulho e preguiça) para os leitores serão o critério fundamental de avaliação das duas filosofias. É igualmente do ponto de vista moral que Epiteto e Montaigne, representantes do dogmatismo e do ceticismo, serão considerados inconciliáveis, visto que a aniquilação recíproca de vícios e virtudes impossibilita a constituição de uma moral perfeita. Nos Pensamentos, o ponto de vista assumido é o da teoria do conhecimento, ainda que se termine o embate no mesmo impasse moral da Conversa. A diferença mais importante, porém, é que os Pensamentos trazem o embate para o interior da natureza humana. Ambas as obras concordam em apontar ao apontar a religião cristã como fonte de explicação para os paradoxos da condição humana.
Myeloid LXR (Liver X Receptor) Deficiency Induces Inflammatory Gene Expression in Foamy Macrophages and Accelerates Atherosclerosis
Authors: Endo-Umeda Kaori , Kim Eunyoung , Thomas David G. , Liu Wenli , Dou Huijuan , Yalcinkaya Mustafa , Abramowicz Sandra , Xiao Tong , Antonson Per , Gustafsson Jan-Åke , Makishima Makoto , Reilly Muredach P. , Wang Nan , Tall Alan R. 
Published: April 2022

Background:
Cholesterol loaded macrophage foam cells are a prominent feature of atherosclerotic plaques. Single-cell RNA sequencing has identified foam cells as TREM2 (triggering receptor expressed on myeloid cells 2) positive populations with low expression of inflammatory genes, resembling the TREM2 positive microglia of neurodegenerative diseases. Cholesterol loading of macrophages in vitro results in activation of LXR (liver X receptor) transcription factors and suppression of inflammatory genes.

Methods:

To test the hypothesis that LXRs mediate anti-inflammatory effects in
Trem2
expressing atherosclerotic plaque foam cells, we performed RNA profiling on plaque cells from hypercholesterolemic mice with myeloid LXR deficiency.

Results:

Myeloid LXR deficiency led to a dramatic increase in atherosclerosis with increased monocyte entry, foam cell formation, and plaque inflammation. Bulk cell-RNA profiling of plaque myeloid cells showed prominent upregulation of inflammatory mediators including oxidative, chemokine, and chemotactic genes. Single-cell RNA sequencing revealed increased numbers of foamy TREM2-expressing macrophages; however, these cells had reduced expression of the
Trem2
gene expression module, including phagocytic and cholesterol efflux genes, and had switched to a proinflammatory and proliferative phenotype. Expression of
Trem2
was suppressed by inflammatory signals but not directly affected by LXR activation in bone marrow-derived macrophages.

Conclusions:

Our current studies reveal the key role of macrophage LXRs in promoting the
Trem2
gene expression program and in suppressing inflammation in foam cells of atherosclerotic plaques.
Abstract 12201: Dissecting the Protective Effect of Phosphodiesterase 3A Hyperactivity on the Heart Using Single Nuclei Sequencing and Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Authors: Mücke Michael B , Pallien Tamara , Dierks Philip , Walker-Gray Ryan , Sholokh Anastasiia , Miller Duncan C , Diecke Sebastian , Hubner Norbert , Klussmann Enno 
Published: April 2022

Mutations causing hyperactivity of phosphodiesterase 3A (PDE3A) lead to autosomal dominant hypertension with brachydactyly type E. Affected patients suffer from profoundly increased blood pressure and, if untreated, die from stroke at the age of 50 years. Surprisingly, the hearts of affected individuals show little signs of hypertrophic responses to these conditions, indicating a beneficial effect of PDE3A hyperactivity on the damaging mechanisms of the heart. PDE3A inhibition was previously tested as a treatment for heart failure. While
PDE3A
inhibitors lead to short term positive inotropy, treatment with these drugs decreases overall survival of heart failure patients, underlining the importance of PDE3A in the heart. As arterial hypertension is a major risk factor for cardiovascular disease and proves to be a major burden on the healthcare system, dissecting the mechanisms underlying the protective effect of PDE3A can open new paths to preventing and treating heart failure.

We hypothesize that PDE3A activation protects the heart from the detrimental effects of high blood pressure
via
spatially distinct regulation of cAMP levels through its different isoforms, leading to an altered calcium handling of cardiomyocytes.

We are focusing on two mutations causing PDE3A hyperactivity, one in a regulatory hotspot the other in the catalytic domain. Using single nuclei sequencing of left ventricles of rats, genetically engineered to recapitulate the human mutations, we are dissecting the differences in the cell type-specific gene regulatory response to high blood pressure compared to the wildtype. To follow up in a human model system, we used genome editing to introduce the mutations of interest into human induced pluripotent stem cells (hiPSC).
hiPSC lines containing PDE3A mutations as well as isogenic matched control hiPSCs were differentiated to generate hiPSC-derived cardiomyocytes (hiPSC-CMs). Preliminary data from a well-established 2D system show significantly altered calcium handling of PDE3A-mutant hiPSC-CMs. Performing RNA-sequencing, calcium imaging and cAMP measurements in isogenic hiPSC-CMs, we are functionally characterizing the effect of PDE3A activity on cardiomyocyte profile and physiology to a high resolution.
Abstract 13619: Obese Patients With New Onset Atrial Fibrillation/Flutter Have Higher Rates of Hospitalization, Cardioversion, and Ablation
Authors: Norton Jonathan , Foy Andrew , Naccarelli Gerald 
Published: April 2022

Atrial fibrillation (AF) is the most common arrhythmia and can lead to frequent healthcare encounters. Obesity is a rapidly growing epidemic, and greater than one third of all adults in the USA are obese. Additionally, obese patients have over twice the prevalence of AF compared to the non-obese. Despite the frequency of these conditions, there is limited research assessing the relationship between obesity and healthcare resource utilization (HRU) in AF patients.
We hypothesized that obese patients with new onset atrial fibrillation/flutter (AFL) would have higher AF/AFL related hospitalizations and procedures compared to non-obese patients.
We utilized MarketScan® claims data to select patients with new onset atrial fibrillation or atrial flutter in 2017 and 2018 and classified them according to diagnosis codes as obese or non-obese. We then stratified by the diagnosis of obesity.
There were 95,314 patients with new onset atrial fibrillation/flutter, which included 72,218 (76%) who were non-obese and 23,096 (24%) who were obese. There were significantly more males than females (64.4% vs. 35.6%). The average age was similar at 54.4 (+/- 0.04) in the non-obese and 54.6 (+/- 0.06) in the obese cohort. The rate of hospitalizations (35.9% vs. 24.8%, p<0.001), cardioversions (16% vs. 10%, p<0.001), and ablation procedures (10% vs. 7%, p<0.0001) were significantly higher in the obese versus non-obese cohort.
Obese patients with new onset AF/AFL had significantly higher HRU than non-obese patients. This increased health resource utilization increases the cost of care in such patients and future studies should concentrate on understanding the reasons for this difference.
Abstract 13810: Diabetes versus Cardiovascular Mortality in the Context of Food Insecurity Among United States Adults
Authors: Banerjee Srikanta K , Dunn Patrick 
Published: April 2022

Food insecurity has become an important global concern with close associations with cardiovascular disease. However, how food insecurity affects individuals with long-term diabetes is currently unknown. In this study, we assessed the hypothesis that positive diabetes history modifies the effect of food insecurity on cardiovascular disease (CVD) mortality.
We analyzed data on adults (≥ 20 years) from the 1999-2010 National Health and Nutrition Examination Survey, with mortality data obtained through 2015. The causes of death for adults (N=4037) were defined using the International Classification of Disease coding (ICD-10). Complex Samples Cox regression was used to assess if diabetes status modified the relationship between food insecurity and CVD-mortality.
During a 11.1-year follow-up, among adults with diabetes, 184 individuals died (15%). The hazard ratio (HR) for CVD-mortality among adults with food insecurity compared with adults without food insecurity, with adjustment for age and gender only, was 1.66; 95% confidence interval [CI: 1.26, 2.20]. The adjusted HR was elevated, 2.13 (CI 1.30-3.48, p < 0.05), among adults with diabetes and food insecurity but close to 1.0 (1.57 CI 0.97-2.54, p = 0.07) among adults with only food insecurity after the results were controlled for medical risk factors (chronic kidney disease (CKD) and obesity) and demographic risk factors (age, gender, ethnicity, education).
In conclusion, individuals who are food-insecure and have diabetes have a higher probability of death from cardiovascular disease in long-term follow-up than those individuals without diabetes. Risk scoring and CVD prevention strategies should include screening and surveillance for food insecurity status. Comprehensive and interdisciplinary approaches to reducing cardiovascular disease-related disparities and health risks should be implemented through equity research.
The Polar Transition from Alpha to Beta Regions Set by a Surface Buoyancy Flux Inversion
Authors: Caneill Romain , Roquet Fabien , Madec Gurvan , Nycander Jonas 
Published: April 2022

Abstract
The stratification is primarily controlled by temperature in subtropical regions (alpha ocean) and by salinity in subpolar regions (beta ocean). Between these two regions lies a transition zone, often characterized by deep mixed layers in winter and responsible for the ventilation of intermediate or deep layers. While of primary interest, no consensus on what controls its position exists yet. Among the potential candidates, we find the wind distribution, air–sea fluxes, or the nonlinear cabbeling effect. Using an ocean general circulation model in an idealized basin configuration, a sensitivity analysis is performed testing different equations of state. More precisely, the thermal expansion coefficient (TEC) temperature dependence is explored, changing the impact of heat fluxes on buoyancy fluxes in a series of experiments. The polar transition zone is found to be located at the position where the sign of the surface buoyancy flux reverses to become positive, in the subpolar region, while wind or cabbeling are likely of secondary importance. This inversion becomes possible because the TEC is reducing at low temperature, enhancing in return the relative impact of freshwater fluxes on the buoyancy forcing at high latitudes. When the TEC is made artificially larger at low temperature, the freshwater flux required to produce a positive buoyancy flux increases and the polar transition moves poleward. These experimets demonstrate the important role of competing heat and freshwater fluxes in setting the position of the transition zone. This competition is primarily influenced by the spatial variations of the TEC linked to meridional variations of the surface temperature.
Trajectories of Insomnia Symptoms Among Aging Employees and Their Associations With Memory, Learning Ability, and Concentration After Retirement - A Prospective Cohort Study (2000–2017)
Authors: Etholén Antti , Pietiläinen Olli , Kouvonen Anne , Hänninen Mirja , Rahkonen Ossi , Lallukka Tea 
Published: April 2022

Objectives We applied a person-oriented approach and used latent class linear mixed models to identify sleep trajectories that explain memory, concentration, and learning ability problems after retirement. Methods Data consist of prospective surveys from four phases of the Helsinki Health Study between 2000–2017 (n = 3748, aged 55–77 years, 80% women). Multinomial regression was used to examine the associations between sleep trajectories and cognitive function, adjusting for sociodemographic, health-related behavior, and health factor covariates. Results Among statutory retirees, three latent group trajectories of insomnia-related symptoms were identified: stable low, decreasing, and increasing. Among those who had retired for disability reasons, we identified one additional latent group trajectory: stable high. Insomnia symptoms were associated with worse cognitive function. Discussion Early detection of insomnia symptoms would be a potential intervention point to improve both sleep quality and prevent cognitive decline in later life. However, intervention studies are needed.
Association of miR-9-5p and NFIC in the progression of gastric cancer
Authors: Lv Shihong , Liu Lei , Yang Baijing , Zhao Xiaohua 
Published: April 2022

Background Gastric cancer is the most common malignant neoplasm of digestive system. Herein, we aim to detect the expression of nuclear factor I C (NFIC) in gastric cancer cells, and to explore the effect and mechanism of its expression on the development of gastric cancer. Methods qPCR and Western blot assays were carried out to detect NFIC expression. Then, BGC-823 and SGC-7901 cell lines were selected to perform the following functional experiments. The function of NFIC on gastric cancer cells was analyzed by biological experiments. The associations between miR-9-5p and NFIC were searched on the bioinformatics website and identified by dual luciferase assay. The effects of miR-9-5p and NFIC on cells were verified by co-transfection experiments. The related genes expression was examined by Western blot. Results A marked augmentation of NFIC was observed in gastric cancer cells. Knockdown of NFIC significantly inhibited the viability, colony formation, invasion, and migration of gastric cancer cells. Overexpression of miR-9-5p obviously suppressed the viability, colony formation, invasion, and migration of gastric cancer cells, and this phenomenon was aggravated by si-NFIC. Additionally, the expression levels of PCNA, vimentin, and Snail were obviously decreased after miR-9-5p mimic or/and si-NFIC treatment. Conclusions These results suggested that NFIC was highly expressed in gastric cancer cells, and knockdown of NFIC suppressed the growth and mobility of gastric cancer cells; miR-9-5p was identified as an upstream regulator of NFIC and suppressed the malignant behaviors of gastric cancer cells by targeting NFIC through affecting PCNA, vimentin, and Snail expression.
Implementation of a Clinical Leadership Curriculum for Pediatric Residents
Authors: Mai Daniel H. , Newton Heather , Farrell Peter R. , Mullan Paul , Kapoor Rupa 
Published: April 2022

Introduction Clinical leadership is an essential skill for physicians, empowering them to lead and coordinate teams, communicate clearly under various conditions, model positive behaviors, display emotional intelligence, and ultimately improve patient care outcomes. However, there are currently no standardized residency curricula or competency-based assessments for clinical leadership, as residents often assimilate leadership skills through trial-and-error or observation of their clinical faculty. By utilizing a comprehensive needs assessment and synthesizing evidence-based practices, we developed and implemented a longitudinal and skills-based clinical leadership curriculum for pediatric residents. Methods We modeled our clinical leadership curriculum after Kern's 6-step approach to curricular development and the Accreditation Council for Graduate Medical Education competency requirements for professionalism. We identified topics based on a resident needs assessment and synthesized evidence from published practices. The curriculum was implemented through both monthly facilitated group sessions and independent learning modules. Results 44 postgraduate year-2 (PGY-2) and PGY-3 pediatric residents participated in at least one monthly session of the clinical leadership curriculum. 27 (61%) completed the survey to evaluate the efficacy of the curriculum. Of the respondents, 23 (85%) residents found the leadership sessions useful, 4 (15%) were neutral, and none (0%) rated the sessions as not useful. 26 (96%) residents reported that the sessions should be continued. Conclusion The clinical leadership curriculum has been received favorably by senior pediatric residents at our institution. Our next steps are to pilot the curriculum within residency programs of different specialties at our own institution as well as with pediatric residencies at other institutions.
“Sax for Sex”: A Brief Narrative Review Exploring the Music-Sex Connect
Authors: Tikka Sai Krishna , Garg Shobit , Pattojoshi Amrit , Tikka Deyashini Lahiri 
Published: April 2022

Context: The assertion that music has an impact on sexual performance is a popular one. However, music is seldom used in clinical settings for enhancement of sexual problems and little is known regarding its scientific proof. Aim: To explore available literature on: the interplay between sex and music in the human evolution; brain basis for music and sex; and studies using music to therapeutically enhance sexual performance. Conclusions: Evidence suggests an evolutionary basis for the relationship between music and sex. There is converging neurobiological understanding that posits both music and sex to have common brain substrates—the reward pathway, diencephalic structures, limbic system, and prefrontal cortex. Psychological mechanisms hypothesized for a positive role of music in enhancing the act of sex need systematic evaluation. The empirical evidence for the use of music as an adjunct strategy in the treatment of sexual dysfunctions, though positive and encouraging, is still sparse.
Central effects of galcanezumab in migraine: a pilot study on Steady State Visual Evoked Potentials and occipital hemodynamic response in migraine patients
Authors: de Tommaso Marina , La Rocca Marianna , Quitadamo Silvia Giovanna , Ricci Katia , Tancredi Giusy , Clemente Livio , Gentile Eleonora , Ammendola Elena , Delussi Marianna 
Published: April 2022

Abstract
Background
The discovery of the prominent action of Calcitonin Gene Related Peptide –CGRP- on trigeminal afferents and meningeal vessels, opened a new era in migraine treatment. However, how the block of nociceptive afferents could act on central mechanisms of migraine is still not clear. In this pilot study we aimed to test the effect of 3 months Galcanezumab (CGA) therapy on occipital visual reactivity in migraine patients, using the Steady State Visual Evoked Potentials-SSVEPs and Functional Near Infrared Spectroscopy –fNIRS.

Method
Thirteen migraine patients underwent clinical and neurophysiological examination in basal condition (T0), 1 h after GCA injection (T1) and after 3 months of GCA treatment (T2). Ten healthy volunteers were also evaluated.

Results
At T2, there was a reduction of headache frequency and disability. At T2, the EEG power significantly diminished as compared to T0 and T1 at occipital sites, and the topographical analysis confirmed a restoration of SSVEPs within normal values. The Oxyhemoglobin levels in occipital cortex, which were basically increased during visual stimulation in migraine patients, reverted to normal values at T2.

Conclusions
The present pilot study indicates that Galcanezumab could act on cortical targets located beyond the pain network, restoring the abnormal occipital reactivity. This effect could indicate the possible disease modifying properties of CGRP related monoclonal antibodies.