bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of tracheal occlusion in congenital diaphragmatic hernia in the nitrofen rat lung explant model
Authors: Miyake Yuichiro , Tse Wai Hei , Wang Jia Qi , Leon Nolan , Mourin Muntahi , Patel Daywin , Aptekmann Arzu Ozturk , Yamataka Atsuyuki , Keijzer Richard 
Published: December 2022

Abstract
Purpose:
Here, we establish a tracheal occlusion (TO) model with rat lung explants in nitrofen-induced pulmonary hypoplasia in congenital diaphragmatic hernia (CDH).
Methods:
We extracted lungs from rats on embryonic day 18. We mimicked TO in the lung explants by tying the trachea. We assessed lung weight, morphometry, and abundance of Ki-67, Active caspase-3, and Prosurfactant Protein C (proSP-C) with immunofluorescence.
Results:
Lung weight was higher in TO+ than TO– on day 1. Abundance of Ki-67 was higher in TO+ than TO– (0.15 vs. 0.32, p=0.009 for day 1, 0.07 vs. 0.17, p=0.004 for day 2, 0.07 vs. 0.12, p=0.044 for day 3), and Active caspase-3 was higher in TO+ than TO– on day 2 and day 3 (0.04 vs. 0.03 p=0.669 for day 1, 0.03 vs. 0.13 p<0.001 for day 2, 0.04 vs. 0.17 p=0.008 for day3). However, proSP-C protein abundance was lower in TO+ than TO– (67.9 vs. 59.1 p=0.033 for day 1, 73.5 vs. 51.6 p=0.038 for day 2, 83.1 vs. 56.4 p=0.009 for day 3).
Conclusions:
The TO model in lung explants mimics the outcomes of current surgical models of TO and further studies can reveal the cellular and molecular effects of TO in CDH lungs.
Invasion strategies and establishment of Ceratium dinoflagellate in subtropical watersheds of Southern Brazil and its interaction with Microcystis cyanobacteria: I. Hydrographic Region of Uruguay
Authors: Silva Ruan Dutra da , Müller Carla Cristine , Scherer Karen Dornelles , Rosa Luana Morais da , Frizzo Juliana Karl , Cardoso Luciana de Souza 
Published: December 2022

Abstract

Ceratium furcoides is a dinoflagellate that exhibits invasive behavior in aquatic ecosystems of Southern Brazil. Because of this, the study had as objectives to determine the factors that drive dinoflagellate populations as well as identifying temporal and spatial patterns of these populations, relating to Microcystis and propose actions against the invasion and establishment of Ceratium. Data (physical and chemical water and phytoplankton density) came from the monitoring carried out by CORSAN (Riograndense Sanitation Company), in reservoirs in the Hydrographic Region (HR) of Uruguay, from 2013 to 2019 (weekly to monthly sampling); climatic data were obtained through INMET (National Meteorological Institute). Seasonally, maximum precipitation occurred in autumn and spring, temperatures followed a subtropical pattern. Regarding phytoplankton, maximum density occurred in autumn and summer, in 2018 and 2013 (except Ceratium, with maximum in 2014). The analysis indicated that the highest values of density of phytoplankton, cyanobacteria, and Microcystis occurred in U20 basin, in Erechim (autumn 2018) and Passo Fundo (summer 2013); Ceratium peaks were found at U70 in Dom Pedrito (summer 2014) and U80 in Aceguá (autumn 2015). Usually, the populations of dinoflagellate and Microcystis did tend to stabilization, however showed classic relations, such as competition and coexistence, and dominance of the dinoflagellate. The results suggest that local environmental characteristics (temperature, phosphorus, nitrate, organic matter, pH) are key for the development of C. furcoides in this HR, and as is in an environment susceptible to its invasion, studies like this are important in understanding and eventually combating species with this character.
Post-feeding molecular responses of cobia (Rachycentron canadum): RNA-sequencing as a tool to evaluate postprandial effects in hepatic lipid metabolism
Authors: Araújo Bruno C. , Barbosa David A. , Honji Renato M. , Branco Giovana S. , Menegidio Fabiano B. , Marques Victor H. , Moreira Renata G. , Kitahara Marcelo V. , Rombenso Artur N. , de Mello Paulo H. , Hilsdorf Alexandre W.S. 
Published: December 2022

Abstract

We used transcriptome sequencing to investigate the hepatic postprandial responses of Rachycentron canadum (cobia), an important commercial fish species. In total, 150 cobia juveniles (50 per tank, triplicate) were fed ad libitum with a commercial diet for 7 days, fasted for 24 h and fed for 10 min. Liver samples were sampled 10 min before and 30 min, 1, 2, 4, 8, 12, and 24 h after the feed event. Posteriorly, it was evaluated liver fatty acid profile, transcriptome sequencing, and differential gene expression focusing on fatty acid synthesis and oxidation pathways. In general, liver fatty acid profile reflected the diet composition. Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and n-3 long chain polyunsaturated fatty acids (n-3 LC-PUFA) levels increased at 8 to 12 h and decreased at 24 h after the feed event. It was observed a high number of differentially expressed genes (DEGs) comparing fish fasted by 8 h with those fasted by 30 min and 24 h, while a reduced number of DEGs was observed comparing individuals fasted by 30 min compared with those fasted by 24 h. Similarly, the main differences in the expression of genes related to the fatty acid biosynthesis and oxidation pathways were noticed in individuals fasted by 8 h compared with those fasted by 30 min and 24 h. The results suggested that the adequate time to sample the individuals ranged between 8 and 12 h after meal, since apparently, after 24 h, differential gene expression was not necessarily influenced by feed intake.
Angiotensin II type 2 receptor (AT 2 R) activation prevents the loss of megalin in the kidney and the onset of proteinuria in high salt fed rats
Authors: Kulkarni Kalyani , Patel Sanket , Ali Riyasat , Hussain Tahir 
Published: December 2022

Abstract
Proteinuria is a risk factor for and consequence of kidney injury. Angiotensin II type 2 receptor (AT2R) is an emerging reno-protective target and is anti-proteinuric under pathological conditions, including high salt-fed obese animals. However, the mechanisms remain unknown, particularly whether the anti-proteinuric activity of AT2R is independent of its anti-hypertensive and anti-inflammatory effects. In the present study, obese Zucker rats were fed high sodium (4%) diet (HSD) for 48 hours, a time in which blood pressure does not change. HSD caused proteinuria without affecting glomerular slit diaphragm proteins (nephrin and podocin), glomerular filtration rate and inflammatory (TNFα, IL-6, and TGF-β), ruling out glomerular injury and inflammation but indicating tubular mechanisms of proteinuria. At cellular and molecular levels, we observed a glycogen synthase kinase (GSK)-3β-mediated megalin phosphorylation, and its subsequent endocytosis and lysosomal degradation in HSD-fed rat kidneys. Megalin is a major proximal tubular endocytic protein transporter. The AT2R agonist C21 (0.3 mg/kg/day, i.p.) administration prevented proteinuria and rescued megalin surface expression potentially by activating Akt-mediated phosphorylation and inactivation of GSK-3β in HSD-fed rat kidneys. Overall, AT2R has a direct anti-proteinuric activity, potentially via megalin regulation, and is suggested as a novel target to limit kidney injury.
Detection of lung cancer using modified co-learning technique based on ten Convolutional Neural Network models in PET/CT image
Authors: R Kishore , R Suresh babu 
Published: December 2022

Abstract
Background: A proposed Lung Cancer Detection System (LCDS) lacks sensitivity and accuracy due to low spatial resolution in Positron Emission Tomography (PET) image and low contrast in Computed Tomography (CT) image.
Method: Such an issue has been resolved by creating a modified co-learning learning technique which will be based on ten Convolutional Neural Network (CNN) (Alexnet, Vgg16, Vgg19, Squeezenet, Googlenet, Inceptionv3, Mobilenetv2, Densenet201, Resnet18, Xception) models. This technique encodes modality specific features and utilizes them to acquire a spatially varying fusion map. These fusion maps are multiplied using modality feature map for an utilization of image analysis.
Result: By the use of modified co-learning technique, this system attained (Densenet201) 97.3% sensitivity, 98.2% accuracy and 1.6 false positives per scan in PET/CT.
Conclusion: A proposed LCDS attained tremendously minimum false positive rate and it is a promising technique in support of cancerous recognition due to improved sensitivity and accuracy.
WITHDRAWN: A Comparative Study of Life-Years Lost Attributable to Air Particulate Matter in Asia-Pacific and European Countries in 2019
Authors: Somboonsin Pattheera , Vardoulakis Sotiris , Canudas-Romo Vladimir 
Published: December 2022

Abstract
Air particulate matter (PM) and its harmful effects on human
health are of great concern globally due to all-cause and
cause-specific mortality impacts across different population
groups. This study has three aims: (1) to investigate life-years
lost (LYL) attributable to PM by causes of death; (2) to assess LYL
across different socio-demographic index (SDI) countries; and (3)
to compare LYL due to PM between Asia-Pacific (APAC) and Europe.
The data used comefrom IHME and Health Effects Institute. Our
results showthat average LYL due to PM in APAC was greater than in
Europe, with some Island countries particularly affected by the
exposure to household air pollution (HAP). Three quarters of LYL
came from premature deaths by ischemic heart disease and stroke, in
both continents. There were significant differences between SDI
groups for causes of death due to ambient PM and HAP. Our findings
call for urgent improvement of clean air to reduce indoor and
outdoor air pollution-related mortality.
Influencing Factors Analysis of Adaptability of Cancer Patients to Return-to-work
Authors: Li Jia-mei , Su Xiao-qin , Xue Ping , Guo Yu-jie 
Published: December 2022

Abstract
Objectives

To clarify the adaptability of cancer patients to return to work and explore its influencing factors.

Design:

A cross-sectional study

Settings/participants:

From March to October 2021, 283 cancer patients in the follow-up period were recruited from the oncology departments of four secondary and above hospitals and cancer friendship associations in Nantong city using self-developed scale of adaptability to return to work for cancer patients by convenience sampling method.

Methods

The contents included general sociodemographic data, disease-related data, cancer patients' readability to work Scale, Medical Coping Style Questionnaire, Social Support Rating Scale, Family Closeness and Readability Scale, General self-efficacy Scale and Social impact Scale. Paper questionnaires were used for face-to-face data collection, and SPSS17.0 was used for statistical analysis. Univariable analyses and multiple linear regression analysis were conducted.

Results

The overall score of cancer patients' adaptability to return to work was (87.05 ± 20.255), (22.54 ± 4.234) for the dimension of focused rehabilitation, (32.02 ± 9.013) for the dimension of reconstruction effectiveness, and (32.49 ± 9.023) for the dimension of adjustment planning. Multiple linear regression analysis showed that the current return to full-time work (β = 0.226, P 0.05), the current return to non-full-time work (β = 0.184, P 0.05), yield response (β = -0.132, P 0.05), and general self-efficacy (β = 0.226, P 0.05) could affect their return to work adaptation.

Conclusion

The results of status quo and influencing factors showed that the adaptability of cancer patients to return to work was generally higher in this study. Patients who had returned to work, had lower yield coping score and higher general self-efficacy had better adaptability to return to work.

Ethical approval

It has been approved by the Human Research Ethics Committee of the Affiliated Hospital of Nantong University (Project No.202065)
IVUS-guided zero-contrast percutaneous coronary intervention with rotational atherectomy: a case report
Authors: Stajic Zoran , Milicevic Dusan , Neskovic Aleksandar N. , Jagic Nikola 
Published: December 2022

Abstract
We hereby presented a case of absolute zero-contrast IVUS-guided PCI with rotational atherectomy of heavily calcified left circumflex stenosis in a patient with previous episode of CI-AKI. To the best of our knowledge, this is the first reported case of zero-contrast IVUS-guided PCI based on previously 6-month old coronary angiogram, considering the fact that the patient has had a previous episode of CI-AKI and did not have any coronary event in the meantime.
Prospective Observational Study on Clinical and Epidemiological Profile of Adult Patients Presenting to the Emergency Medicine department with Upper Gastrointestinal Bleed
Authors: Raj Alok , Kaeley Nidhi , Prasad Hari , Patnaik Itish , Bahurupi Yogesh , Joshi Shrirang , Shukla Krishna , Galagali Santosh , Patel Sanket 
Published: December 2022

Abstract
Background and objective

Bleeding from the upper gastrointestinal tract is one of the common medical emergencies. In this study, we assessed patients' socio-demographic and clinical characteristics and the association of clinical characteristics with treatment outcomes among patients with upper gastrointestinal bleed (UGIB) presenting to the emergency medicine department. At present, there is a scarcity of data on upper GI bleed in Northern part of India.

Material and method

The study was a single-center, prospective observational study conducted at an urban tertiary care center. Consecutive patients with UGIB were enrolled in the study from August 2020 to February 2022. A detailed history was obtained, including demographic data such as age and sex, presenting complaints, history of presenting illness, history related to co-morbidities, addiction, and drug history. Pre-endoscopic Rockall and Glasgow-Blatchford Score were calculated for each patient. The patients were subsequently followed up till discharge from the hospital. The final outcomes with regard to mortality, need for blood transfusion, length of emergency department (ED), and hospital stay and discharge were noted.

Result

141 patients were included in the study. The mean age of the patients with UGIB was 48.62 ± 14.63 years. 115(81.6%) patients were male compared to 26(18.4%) Females. The most common co-morbidity was chronic liver disease (40;28.4%). The most frequent presenting complaint in this study was hematemesis (96; 68.1%), followed by melena (76;53.9%). The mean (SD) of the Rockall Score was 2.46 ± 1.75. The mean (SD) of the Glasgow Blatchford Score was 12.46 ± 3.15 in patients with upper gastrointestinal bleed.

Conclusion

In our study, hematemesis was the most prevalent symptom of an upper GI bleed, followed by melena. Portal hypertension was the more common cause of upper GI bleed. Alcohol intake, NSAIDs abuse, and co-morbidities such as underlying chronic liver disease, hypertension, and diabetes are independent risk factors in patients with upper GI bleed. Early upper GI endoscopy can lower the morbidity, mortality, and length of hospital stay in patients with upper GI bleed.
Effects of Saline Soil Characteristics on Plant Growth Environment in Cultivated Depth of the Yellow River Delta
Authors: Qin Peng , Liu Zihao , Luan Yiquan , Liu Chuanxiao 
Published: December 2022

Abstract
This study combines the soil salinity determination, pH determination, ion content determination, particle size distribution (PSD), X-ray diffraction and Mercury intrusion Porosimetry (MIP) were used to quantitatively analyze the characteristics of saline-alkali soil within the cultivation depth of the Yellow River Delta, to explore the influence of the characteristics of saline-alkali soil in the coastal area on the plant growth environment. The results showed that the salinity content of surface saline-alkali soil in the Yellow River Delta was as high as 11.39 g/kg, which was 10 times higher than that of normal soil. The extremely high salinity affected plant growth through direct and indirect salinity damage. Soil pH, ion content, soil texture and mineral composition all affect plant growth. In the coastal saline-alkali soil of the Yellow River Delta, 98% of pores are inactive pores and capillary pores with a pore size of 0.1 to 10 and the pore distribution is regular. The study on the characteristics of saline-alkali soil in the Yellow River Delta can analyze the effects of saline-alkali soil on plant growth environment from physical, chemical and microscopic properties, and is of great significance to the treatment of saline-alkali soil in coastal areas.
AMPK Activation Improves Recovery from Pneumonia-Induced Lung Injury via Reduction of ER-Stress and Apoptosis in Alveolar Epithelial Cells
Authors: Becker Eugene , Husain Maroof , Bone Nathaniel , Smith Samuel , Zmijewski Jaroslaw W. 
Published: December 2022

Abstract
Background
Bacterial pneumonia and related lung injury are among the most frequent causes of mortality in intensive care units, but also inflict serious and prolonged respiratory complications among survivors. Given that endoplasmic reticulum (ER) stress is a hallmark of sepsis-related alveolar epithelial cell (AEC) dysfunction, we tested if AMP-activated protein kinase (AMPK) affects recovery from ER stress and apoptosis of AECs during post-bacterial infection.
Methods
In a murine model of lung injury by P. aeruginosa non-lethal infection, therapeutic interventions included AMPK activator metformin or GSK-3β inhibitor Tideglusib for 96 hours. Recovery from AEC injury was evidenced by accumulation of soluble T-1α (AEC Type 1 marker) in BAL fluids along with fluorescence analysis of ER-stress (CHOP) and apoptosis (TUNEL) in lung sections. AMPK phosphorylation status was determined via Immunoblot analysis from lung homogenates. Macrophage-dependent clearance of apoptotic cells was determined using flow cytometry assay.
Results
P. aeruginosa-induced lung injury resulted in accumulation of neutrophils and cellular debris in the alveolar space along with persistent (96 hours) ER-stress and apoptosis of AECs. While lung infection triggered AMPK inactivation (de-phosphorylation of Thr172-AMPK), metformin and Tideglusib promptly restored the AMPK activation status. In post infected mice, AMPK activation reduced indices of lung injury, ER stress and related apoptosis of AECs, as early as 24 hours post administration of AMPK activators. In addition, we demonstrate that the extent of apoptotic cell accumulation is also dependent on AMPK-mediated clearance of apoptotic cells by macrophages.
Conclusions
Our study provides important insights into AMPK function in the preservation of AEC viability after bacterial infection, in particular due reduction of ER-stress and apoptosis, thereby promoting effective recovery from lung injury after pneumonia.
The overexpression of ATPS1 gene, a homodimeric enzyme involved in sulfur assimilation, confers Fe-deficient tolerance in Malus halliana
Authors: Cheng Jiao , Zhang Zhongxing , Gao Yanlong , Dong Yongjuan , Xian Xulin , Li Cailong , Ding Liang , Wang Yanxiu 
Published: December 2022

Abstract
Iron (Fe) deficiency is one of the most common micronutrient deficiencies limiting crop production globally, especially in arid regions because of decreased availability of iron in alkaline soils. S supply increases Fe availability to plants, ATP sulphatase plays an important role in the synthesis of organic sulfur in plants, which is considered to be an important rate-limiting enzyme in sulphur uptake. However, the mechanism of whether it responds to Fe deficiency in plants remains unclear. Here, we identified a ATP sulphatase gene MhATPS1 from Malus hallinan based on qRT-PCR and then genetically transformed it into tobacco and apple calli, which showed that transgenic tobacco and overexpressed apple calli secreted more H+ content compared to the wild type (WT), resulting in increased inter-root acidification. In addition, the transgenic tobacco and apple calli showed better growth under Fe deficiency conditions. Furthermore, increased Fe2+ content and ferric chelate reductase (FCR) activity in the transgenic tobacco and overexpressed calli indicated improved Fe uptake, which is also confirmed by up-regulation of Fe uptake genes, e.g. FRO2, IRT1, FER and FIT. Under Fe deficiency stress, having more strengthened antioxidant capacity in transgenic tobacco, and the same results were shown in overexpressed apple calli. At last, the NBT and DAB staining results also verified the greater scavenging capacity of reactive oxygen species in transgenic tobacco. In summary, the MhATPS1 gene may play a positive role in Fe deficiency stress both tobacco and apple calli.
Cloud Computing and Security in Virtualization Platform
Authors: Shaan Shaan Ishfar Ann , Rumi Masba Uddin Rumi , Anik Anik Dutta , Rubayet Md. Rubayet Hasan , Alauddin Md Alauddin Mazumder 
Published: December 2022

Abstract
Despite of fast advancements in the field of cloud computing, still security is one of the primary barriers to cloud computing adoption. Security as well as effective management of data center are also major concerns. Because virtualization is a basic technology for cloud computing, any cloud vulnerabilities and threats have an impact on virtualization. This paper proposes a secured data center design with cloud virtualization that offers user end protection. It also incorporates the load balancing feature, microservice with containerization and storage management with scalability. Moreover, this paper will also focus on managing the cloud data center effectively and eliminating inside and outside attacks by using zero trust security model. The proposed design ensures disaster recovery, data redundancy, and high availability of data. Access for the militarized and demilitarized zones are filtered to enhance the security of the data center. All the components used in the design of the data center are described elaborately. This design will overcome the current challenges that the industry is facing.
Increased risk of arrhythmias in active acromegaly with complications and persistent uncontrolled active acromegaly
Authors: Fang Qiuyue , Liu Yulou , Li Chuzhong , Gui Songbai , Zhang Yazhuo 
Published: December 2022

Abstract
Objective: Previous studies showed acromegaly have significant higher prevalence of ventricular arrhythmias and often complicated by diabetes mellitus (DM) and hypertension (HT). Both HT and DM are notoriously associated with the development of arrhythmias. However, the effect of complication (DM and/or HT) in acromegaly on ventricular arrhythmias and the risk of ventricular arrhythmias in acromegaly accept therapy but no control is largely unknown.
Methods: A cross-sectional study with 307 acromegaly and 303 patients with non-functional pituitary adenoma as control group. All subjects were divided into acromegaly with/without complication and controls with/without complication. In the longitudinal study, 30 persistent uncontrolled active acromegaly with at least three months follow-up. Electrocardiographic Measurements, laboratory examination, and clinical data collection were performed in all subjects. QT interval corrected for heart rate (QTc) was analyzed among groups.
Results: QTc in acromegaly population significantly increased compared to controls (p<0.001). Factorial design two-way ANOVA correcting age revealed significant main effects of complication (p=0.016) and acromegaly (p<0.0001), as well as positive interactions between complication and acromegaly (P<0.038) on QTc. Persistent uncontrolled active acromegalic patients after therapy showed QTc significantly increase in follow-up relative to pre-treatment (p<0.0001). The normalized GH level (r=0.11, p<0.05) and complication (r=0.25, p<0.0001) have a significant positive correlation with QTc in acromegaly.
Conclusions: Acromegaly is an independent risk factor for ventricular arrhythmias and acromegaly with complication have an elevated risk for ventricular arrhythmia. Persistent uncontrolled acromegaly, who have significantly decreased in serum GH/IGF-1 levels relative to pre-treatment, also enhance the risk of ventricular arrhythmia.