bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bio-based sustainable composites from hazelnut shell and PP/SEBS blends
Authors: Cetin Mukaddes Sevval , Ipek Melek , Fidan Gulsah , Toprakci Ozan , Karahan Toprakci Hatice Aylin 
Published: July 2022

In this study, waste hazelnut shells (HNS) were powderized and used as the filler for the fabrication of sustainable polymeric composites. HNS based composites can be used for many applications because of their tunable intrinsic brown color for various indoor and outdoor applications including decking applications and furniture that require a natural esthetic appearance. Polypropylene (PP), styrene-b-(ethylene-co-butylene)-b-styrene (SEBS), and 50/50 PP/SEBS blends were used as the polymeric matrixes. HNS concentration was set to 2.5, 5, and 10 wt%. PP/SEBS blend and HNS filled granules were prepared by melt mixing in an extruder. Following that polymeric films were fabricated from the granules by compression molding. The morphological, structural, thermal, mechanical, contact angle, water absorption analyses were performed in order to characterize the composites. Matrix type and filler concentration were found significant for the properties. PP/SEBS blends showed homogeneous morphology. At low concentrations, fillers were well-dispersed. On the other hand, at 10 wt% HNS loading agglomerations were observed and mechanical properties showed a noticeable drop. PP-based composites showed the highest tensile strength and elastic modulus with the lowest tensile strain. SEBS-based composites showed the lowest tensile strength and elastic modulus with the highest tensile strain. PP/SEBS composites showed mechanical properties between PP and SEBS. The incorporation of SEBS into PP increased the flexibility of the composites and resulted in relatively higher strain at break.
Utilisation of Janani Shishu Suraksha Karyakaram Services in the State of Telangana: A Rural–Urban Comparison
Authors: Chowhan Nanditha , Sodani Prahlad Rai , Mehrotra Anupam , Bandhu Ashish 
Published: July 2022

Background: Maternal and infant mortality remains one of the concerns in India, even in high-performing states like Telangana. However, the urban–rural divide in the maternal and child health service provided and utilisation by the population is more concerning. The Government of India launched schemes like Janani Suraksha Yojana (JSY) and Janani Shishu Suraksha Karyakaram (JSSK) to improve institutional delivery and reduce the out-of-pocket expenditure of users seeking service at public institutions and private institutions facilities. This study aims to study the utilisation of one such service, that is, JSSK, and compare it in urban and rural areas of Telangana state. Methodology: A cross-sectional study was conducted in Warangal and Ranga Reddy districts in the state of Telangana with an interview schedule administered to mothers who had at least one child at the time of the interview and were beneficiaries of JSY and JSSK from the previous delivery. A multistage cluster random sampling was used to select the study area, and a total of 408 respondents were chosen to participate in the study. Results: The study found that most facility characteristics related to maternal and child health, JSSK service availability and JSSK service satisfaction and awareness were significantly different across the urban and rural areas. Conclusion: The study found that crucial factors affecting respondents’ services utilisation differed across urban and rural areas. This study shows there is still a margin of improvement in services provided under the JSY and JSSK, especially in service availability and awareness of the population on their entitlements under these schemes.
Mid- to Long-Term Outcomes After Medial Open-Wedge High Tibial Osteotomy in Patients With Radiological Kissing Lesion
Authors: Kim Kang-Il , Kim Jun-Ho , Lee Sang-Hak , Song Sang-Jun , Jo Myeong-Guk 
Published: July 2022

Background: Although medial open-wedge high tibial osteotomy (MOWHTO) is the treatment of choice for patients with mild to moderate osteoarthritis with varus malalignment, concerns about inferior outcomes in patients with preoperative radiological kissing lesion (RKL) remain. Purpose: To compare the mid- to long-term clinical and radiological results and survivorship after MOWHTO in patients with versus without preoperative RKL. Study Design: Cohort study; Level of evidence, 3. Methods: Included were 122 knees in patients who underwent MOWHTO with a medial locked plate and had minimum 5-year follow-up data. The mean age at surgery was 55.9 years (range, 38-65 years), and the mean follow-up was 7.5 years (range, 5-12.8 years). All patients had undergone second-look arthroscopy around 2 years after MOWHTO. The knees were divided into an RKL group (n = 17) and no-RKL group (n = 105) based on preoperative standing radiographs. The authors compared postoperative American Knee Society (AKS) knee and function scores, range of motion, and improvements in AKS scores between groups, as well as hip-knee-ankle angle, medial proximal tibial angle, and joint-line convergence angle from preoperatively to postoperatively. Also compared were the degree of cartilage regeneration between first- and second-look arthroscopy and the survival rate after index surgery. Results: Preoperative AKS scores were significantly lower in the RKL group versus the no-RKL group (AKS knee, 79.6 ± 7.5 vs 83.8 ± 3.9, P = .037; AKS function, 68.8 ± 9.3 vs 76.0 ± 5.1, P = .006). Likewise, postoperative AKS scores were significantly lower in the RKL group versus the no-RKL group (AKS knee: 91.3 ± 4.2 vs 94.4 ± 1.6, respectively, P = .008; AKS function: 90.0 ± 10.0 vs 97.6 ± 4.5, respectively, P = .007). However, all patients had excellent postoperative AKS knee and function scores (>80). Moreover, there were no between-group differences in pre- to postoperative improvement in AKS scores, postoperative radiological changes, or grade of cartilage regeneration. The survival rates in the RKL and no-RKL groups were 100% and 97.1%, respectively ( P ≥ .999). Conclusion: Although the latest clinical scores were lower in the RKL group than in the no-RKL group, comparable results in postoperative clinical improvement, cartilage regeneration, and survivorship were observed in patients with RKL at mid- to long-term follow-up.
Factors Influencing the Progression of Patellofemoral Articular Cartilage Damage After Anterior Cruciate Ligament Reconstruction
Authors: Huang Hui , Li Zhengzhao , Luo Shishi , Zheng Jiaxuan , Zhou Gang , Wang Guangji 
Published: July 2022

Background: Although anterior cruciate ligament reconstruction (ACLR) can restore the stability and function of the knee joint, patellofemoral joint cartilage damage still progresses. Currently, the clinically important factors that lead to the progression of patellofemoral articular cartilage damage are not fully understood. Purpose: To investigate the factors that affect the progression of patellofemoral articular cartilage damage after ACLR. Study Design: Cohort study; Level of evidence, 2. Methods: Among 160 patients who underwent ACLR between January 2015 and December 2019, the authors evaluated 129 patients for at least 1 year after surgery. Within 1 week before ACLR and at the last follow-up, patients underwent subjective functional assessment and magnetic resonance imaging evaluations of articular cartilage damage (modified Outerbridge assessment). At the last follow-up, the side-to-side difference on KT-2000 arthrometer and bilateral quadriceps muscle strength were measured. Univariate and multivariate logistic regression analyses were performed. Results: The mean follow-up was 24.69 ± 10.74 months. Progression of patellar cartilage damage from preoperatively to final follow-up was seen in 45 patients ( P < .001). Logistic regression analysis revealed that the follow-up period ( P = .047; odds radio (OR) = 0.953) (improvement of patellar cartilage damage with longer follow-up), partial lateral meniscal resection ( P = .004; OR = 6.929), partial medial meniscal resection ( P = .004; OR = 6.032), and quadriceps muscle strength <80% of the contralateral side ( P = .001; OR = 4.745) were risk factors for the progression of patellar cartilage damage. Conclusion: Cartilage damage at the patellofemoral joint, especially the patellar cartilage, still progresses after ACLR. At a mean follow-up of 24.69 months after ACLR, partial meniscal resection and quadriceps femoris muscle strength were found to be the main risk factors for the progression of patellofemoral articular cartilage damage after ACLR.
Detection of cell markers from single cell RNA-seq with sc2marker
Authors: Li Ronghui , Banjanin Bella , Schneider Rebekka K. , Costa Ivan G. 
Published: July 2022

Abstract
Background
Single-cell RNA sequencing (scRNA-seq) allows the detection of rare cell types in complex tissues. The detection of markers for rare cell types is useful for further biological analysis of, for example, flow cytometry and imaging data sets for either physical isolation or spatial characterization of these cells. However, only a few computational approaches consider the problem of selecting specific marker genes from scRNA-seq data.

Results
Here, we propose sc2marker, which is based on the maximum margin index and a database of proteins with antibodies, to select markers for flow cytometry or imaging. We evaluated the performances of sc2marker and competing methods in ranking known markers in scRNA-seq data of immune and stromal cells. The results showed that sc2marker performed better than the competing methods in accuracy, while having a competitive running time.
Genome-wide identification and expression analysis of the ftsH protein family and its response to abiotic stress in Nicotiana tabacum L
Authors: Pu Tianxiunan , Mo Zejun , Su Long , Yang Jing , Wan Ke , Wang Linqi , Liu Renxiang , Liu Yang 
Published: July 2022

Abstract
Background
The filamentous temperature-sensitive H protease (ftsH) gene family plays an important role in plant growth and development. FtsH proteins belong to the AAA protease family. Studies have shown that it is a key gene for plant chloroplast development and photosynthesis regulation. In addition, the ftsH gene is also involved in plant response to stress. At present, the research and analysis of the ftsH gene family are conducted in microorganisms such as Escherichia coli and Oenococcus and various plants such as Arabidopsis, pear, rice, and corn. However, analysis reports on ftsH genes from tobacco (Nicotiana tabacum L.), an important model plant, are still lacking. Since ftsH genes regulate plant growth and development, it has become necessary to systematically study this gene in an economically important plant like tobacco.

Results
This is the first study to analyze the ftsH gene from Nicotiana tabacum L. K326 (NtftsH). We identified 20 ftsH genes from the whole genome sequence, renamed them according to their chromosomal locations, and divided them into eight subfamilies. These 20 NtftsH genes were unevenly distributed across the 24 chromosomes. We found four pairs of fragment duplications. We further investigated the collinearity between these genes and related genes in five other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis identified differential expression patterns of NtftsH in different tissues and under various abiotic stress conditions.

Conclusions
This study provides a comprehensive analysis of the NtftsH gene family. The exon–intron structure and motif composition are highly similar in NtftsH genes that belong to the same evolutionary tree branch. Homology analysis and phylogenetic comparison of ftsH genes from several different plants provide valuable clues for studying the evolutionary characteristics of NtftsH genes. The NtftsH genes play important roles in plant growth and development, revealed by their expression levels in different tissues as well as under different stress conditions. Gene expression and phylogenetic analyses will provide the basis for the functional analysis of NtftsH genes. These results provide a valuable resource for a better understanding of the biological role of the ftsH genes in the tobacco plant.
Transcriptome analysis and characterization of genes associated to leaf tannin content in foxtail millet [Setaria italica (L.) P. Beauv.]
Authors: Li Suying , Cui Yanjiao , Liu Dan , Zhao Zilong , Zhang Jing , Liu Zhengli 
Published: July 2022

Abstract
Background
Chinese chestnut is an economically important tree species whose yield and quality are seriously affected by red spider attack. Tannins is one of the most important class secondary metabolites in plants, and is closely associated with plant defense mechanisms against insect and herbivory. In our previous studies, it was revealed that several low-tannin foxtail millet varieties growing under the Chinese chestnut trees could attract red spiders to feed on their leaves and protect the chestnut trees from the infestation of red spiders, meanwhile, the growth and yield of foxtail millet plants themselves were not greatly affected.

Results
To identify genes related to leaf tannin content and selection of foxtail millet germplasm resources with low tannin content for interplanting with Chinese chestnut and preventing the red spider attack, the leaves of 4 varieties with different levels of tannin content were harvested for comparative transcriptome analysis. In total, 335 differentially expressed genes (DEGs) were identified. For acquisition of gene functions and biological pathways they involved in, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses were performed, and several DEGs were found to possibly participate in the tannins biosynthesis pathway and transport processes of precursors. In addition, according to the PlantTFDB database, some transcription factors were predicted among the DEGs, suggesting their role in regulation of tannins biosynthesis pathway.

Conclusion
Our results provide valuable gene resources for understanding the biosynthesis and regulation mechanisms of tannins in foxtail millet, and pave the way for speeding up the breeding of low-tannin varieties through marker-assisted selection, which could be utilized for interplanting with Chinese chestnut trees to confer protection against red spider attack.
Phylogenetic analysis and comparative genomics of SARS-CoV-2 from survivor and non-survivor COVID-19 patients in Cordoba, Argentina
Authors: Olivero Nadia B. , Gonzalez-Reiche Ana S. , Re Viviana E. , Castro Gonzalo M. , Pisano María B. , Sicilia Paola , Barbas María G. , Khan Zenab , van de Guchte Adriana , Dutta Jayeeta , Cortes Paulo R. , Hernandez-Morfa Mirelys , Zappia Victoria E. , Ortiz Lucia , Geiger Ginger , Rajao Daniela , Perez Daniel R. , van Bakel Harm , Echenique Jose 
Published: July 2022

Abstract
Background
The SARS-CoV-2 virus is responsible for the COVID-19 pandemic. To better understand the evolution of SARS-CoV-2 early in the pandemic in the Province of Cordoba, Argentina, we performed a comparative genomic analysis of SARS-CoV-2 strains detected in survivors and non-survivors of COVID-19. We also carried out an epidemiological study to find a possible association between the symptoms and comorbidities of these patients with their clinical outcomes.

Results
A representative sampling was performed in different cities in the Province of Cordoba. Ten and nine complete SARS-CoV-2 genomes were obtained by next-generation sequencing of nasopharyngeal specimens from non-survivors and survivors, respectively. Phylogenetic and phylodynamic analyses revealed multiple introductions of the most common lineages in South America, including B.1, B.1.1.1, B.1.499, and N.3. Fifty-six mutations were identified, with 14% of those in common between the non-survivor and survivor groups. Specific SARS-CoV-2 mutations for survivors constituted 25% whereas for non-survivors they were 41% of the repertoire, indicating partial selectivity. The non-survivors’ variants showed higher diversity in 9 genes, with a majority in Nsp3, while the survivors’ variants were detected in 5 genes, with a higher incidence in the Spike protein. At least one comorbidity was present in 60% of non-survivor patients and 33% of survivors. Age 75–85 years (p = 0.018) and hospitalization (p = 0.019) were associated with non-survivor patients. Related to the most common symptoms, the prevalence of fever was similar in both groups, while dyspnea was more frequent among non-survivors and cough among survivors.

Conclusions
This study describes the association of clinical characteristics with the clinical outcomes of survivors and non-survivors of COVID-19 patients, and the specific mutations found in the genome sequences of SARS-CoV-2 in each patient group. Future research on the functional characterization of novel mutations should be performed to understand the role of these variations in SARS-CoV-2 pathogenesis and COVID-19 disease outcomes. These results add new genomic data to better understand the evolution of the SARS-CoV-2 variants that spread in Argentina during the first wave of the COVID-19 pandemic.
Risk factors associated with reintubations in children undergoing foreign body removal using flexible bronchoscopy: a single-center retrospective cross-sectional study
Authors: Zhang Su-Jing , Gu Hong-Bin , Zhou Min , Lin Min-Yi , Zhang Long-Xin , Chen Xiu-Ying , Lu Guo-Lin 
Published: July 2022

Abstract
Background
Reintubation is a severe complication during foreign body (FB) removal that uses flexible bronchoscopy.

Objective
To investigate the incidence and risk factors for reintubations in children undergoing FB extraction by flexible bronchoscopy in a single center.

Design
A retrospective cross-sectional study.

Setting
All children with foreign body aspiration at Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University from January 2015 to December 2020.

Patients
Children with FB removal using a flexible bronchoscopy were enrolled in the trial according to the inclusion criteria.

Measurements
Both multivariable and logistic regression analyses were used to analyze the association between characteristic data and reintubations. The results were presented as odds ratios (ORs) with 95% confidence intervals (CIs).

Results
In total, 244 patients met with the inclusion criteria and were included in the analysis. Among those participants, 28 children (11.5%) underwent reintubations after FB removal by flexible bronchoscopy. Independent factors associated with reintubations were identified as operative time ≥ 60 min [OR: 3.68, 95% CI (1.64–8.82)] and ASA ≥ III [OR: 5.7, 95% CI (1.23–26.4)].

Conclusions
Children undergoing FB removal by a flexible bronchoscopy may encounter with a high incidence of postoperative reintubations. Both long operative duration and a severe physical status cause a growing risk of reintubations.
Fecal microbiota transplantation in the treatment of irritable bowel syndrome: a single-center prospective study in Japan
Authors: Hamazaki Motonobu , Sawada Tsunaki , Yamamura Takeshi , Maeda Keiko , Mizutani Yasuyuki , Ishikawa Eri , Furune Satoshi , Yamamoto Kenta , Ishikawa Takuya , Kakushima Naomi , Furukawa Kazuhiro , Ohno Eizaburo , Honda Takashi , Kawashima Hiroki , Ishigami Masatoshi , Nakamura Masanao , Fujishiro Mitsuhiro 
Published: July 2022

Abstract
Background
Fecal microbiota transplantation (FMT) is a potential treatment for irritable bowel syndrome (IBS), but its efficacy in Japanese IBS patients is unknown. This study aimed to evaluate the efficacy, side effects, and microbiome changes following FMT in Japanese IBS patients.

Methods
Seventeen Japanese patients with refractory IBS received FMT (4 donors) under colonoscopy. Responders were defined by an improvement in the IBS severity index (IBS-SI) of 50 points or more after 12 weeks. We evaluated the IBS-SI and Bristol Stool Form Scale (BSFS) and compared the diversity and microbiome before and 12 weeks after FMT. For the microbiome, we analyzed the V3–V4 region of the 16S rRNA gene.

Results
IBS-SI decreased an average of 115.58 points after 12 weeks, and 10 patients (58.8%) were considered responders. Eight patients with diarrhea (66.7%) and three patients with constipation (60.0%) showed improvement in the BSFS. Two patients complained of mild abdominal pain, but there were no cases with severe side-effects. α-diversity was increased only in the responder group (p = 0.017). Patients who closely paralleled the donor microbiome had a higher rate of IBS-SI improvement. The relative abundance of Neisseria and Akkermansia increased and Desulfovibrio and Delftia were decreased in the responder group after FMT.

Conclusions
Following FMT, about 60% of Japanese patients with IBS showed improvement in both the IBS-SI and BSFS, without severe side effects. Increased α-diversity and similarity to the donor microbiome after FMT may be associated with better treatment effects.
Trial registration: This study was registered in the University Hospital Medical Information Network Clinical Trial Registration (UMIN000026363). Registered 31 May 2017, https://rctportal.niph.go.jp/s/detail/um?trial_id=UMIN000026363. The study was registered prospectively.
Pharmacist-led medication reconciliation at patient discharge: a tool to reduce healthcare utilization? an observational study in patients 65 years or older
Authors: Bajeux Emma , Alix Lilian , Cornée Lucie , Barbazan Camille , Mercerolle Marion , Howlett Jennifer , Cruveilhier Vincent , Liné-Iehl Charlotte , Cador Bérangère , Jego Patrick , Gicquel Vincent , Schweyer François-Xavier , Marie Vanessa , Hamonic Stéphanie , Josselin Jean-Michel , Somme Dominique , Hue Benoit 
Published: July 2022

Abstract
Background
Older patients often experience adverse drug events (ADEs) after discharge that may lead to unplanned readmission. Medication Reconciliation (MR) reduces medication errors that lead to ADEs, but results on healthcare utilization are still controversial. This study aimed to assess the effect of MR at discharge (MRd) provided to patients aged over 65 on their unplanned rehospitalization within 30 days and on both patients’ experience of discharge and their knowledge of their medication.

Methods
An observational multicenter prospective study was conducted in 5 hospitals in Brittany, France.

Results
Patients who received both MR on admission (MRa) and MRd did not have significantly fewer deaths, unplanned rehospitalizations and/or emergency visits related to ADEs (OR = 1.6 [0.7 to 3.6]) or whatever the cause (p = 0.960) 30 days after discharge than patients receiving MRa alone. However, patients receiving both MRa and MRd were more likely to feel that their discharge from the hospital was well organized (p = 0.003) and reported more frequently that their community pharmacist received information about their hospital stay (p = 0.036).

Conclusions
This study found no effect of MRd on healthcare utilization 30 days after discharge in patients over 65, but the process improved patients’ experiences of care continuity. Further studies are needed to better understand this positive impact on their drug care pathway in order to improve patients’ ownership of their drugs, which is still insufficient. Improving both the interview step between pharmacist and patient before discharge and the transmission of information from the hospital to primary care professionals is needed to enhance MR effectiveness.

Trial registration
NCT04018781 July 15, 2019.