bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel multimeric sCD19‐streptavidin fusion protein for functional detection and selective expansion of CD19‐targeted CAR‐T cells
Authors: Lian Hui , Jiang Jinhong , Wang Yao , Yu Xiaoxiao , Zheng Rong , Long Jing , Zhou Mengjie , Zhou Shirong , Wei Cheng , Zhao Ai , Gao Jimin 
Published: May 2022

AbstractBackgroundCARs are engineered receptors comprising an immunoglobulin single‐chain variable fragment (scFv) that identifies and binds to the target antigen, a transmembrane domain, and an intracellular T‐cell signaling domain. CD19 is a B lineage‐specific transmembrane glycoprotein and is expressed in more than 95% of B‐cell malignancies. Streptavidin (SA) is a homo‐tetrameric protein derived from Streptomyces avidinii, which can bind four biotin molecules with an extremely high affinity at a Kd value of 10‐15 M.AimsThe aim of the study is to generate a novel soluble multimeric fusion protein, sCD19‐streptavidin (sCD19‐SA) for functional detection and selective expansion of CD19‐targeted CAR‐T cells.MethodsThe fusion proteins CD19‐SA was expressed in CHO cells and purified by use of Ni‐nitrilotriacetic acid agarose beads.ResultsA novel fusion protein (sCD19‐SA) was generated, consisting of the extracellular domain of human CD19 and the core region of SA, and could be used to functionally detect CD19‐targeted CAR‐T cells. Furthermore, this protein was demonstrated to form multimers to activate CAR‐T cells to induce their selective expansion. Importantly, sCD19‐SA‐stimulated CD19‐targeted CAR‐T cells could improve antitumor effects in vivo.ConclusionsOur study has highlighted the potential of utilizing antigen‐SA fusion proteins such as sCD19‐SA for CAR‐T therapy for the functional detection of CAR expression and selective expansion of CAR‐T cells.
KIF11 manipulates SREBP2‐dependent mevalonate cross talk to promote tumor progression in pancreatic ductal adenocarcinoma
Authors: Gu Xiang , Zhu Qunshan , Tian Guangyu , Song Wenbo , Wang Tao , Wang Ali , Chen Xiaojun , Qin Songbing 
Published: May 2022

AbstractCholesterol metabolism is highly correlated with risks of pancreatic ductal adenocarcinoma (PDAC). Nevertheless, the underlying mechanisms of activation of cholesterol biogenesis remain inconclusive. KIF11 is a key component of the bipolar spindle and expresses highly in various malignancies. However, its functional role in PDAC tumorigenesis is still unclear. This study aims to elucidate the oncogenic functions of KIF11 in stimulating cholesterol metabolism, thereby driving PDAC progression. We utilized bioinformatics analysis to identify that KIF11 expressed highly in tumor samples versus paired normal tissues and high KIF11 correlated with high clinical stages of patients. Patients with high KIF11 had worse survival outcomes relative to those with low KIF11. Gene set enrichment analysis (GSEA) revealed that KIF11 correlated intensively with the mevalonate (MVA) metabolic pathway. Positive associations were observed between KIF11 and MVA‐signature (HMGCR, FDFT1, SQLE, and MSMO1). KIF11 could elevate the free cholesterol content of PDAC cells and targeting MVA inhibited the in vitro growth of KIF11‐overexpressing cells. Mechanistically, we found KIF11 could interact with SREBP2, the master regulator of MVA. High KIF11 could increase SREBP2 proteins, but not alter their mRNA levels. KIF11 could attenuate the ubiquitination‐mediated degradation of SREBP2, thereby enhancing its stability and accumulation. Accordingly, KIF11 stimulated the expressions of MVA‐signature and free cholesterol contents depending on SREBP2. In addition, KIF11 depended on SREBP2 to promote cell growth, migration, stemness, and colony formation abilities. The subcutaneous xenograft models indicated that targeting MVA biogenesis (atorvastatin) is effective to restrict the in vivo growth of KIF11high PDAC. Taken together, our study identified that KIF11 could activate the MVA cross talk to drive PDAC progression and inhibiting the KIF11/MVA axis provided a therapeutic vulnerability in the treatment of PDAC.
Construction of ovarian metastasis‐related immune signature predicting prognosis of gastric cancer patients
Authors: Gao Jianpeng , Huo Shiying , Zhang Yu , Zhao Zhenxiong , Pan Hongda , Liu Xiaowen 
Published: May 2022

AbstractBackgroundOvarian metastasis (OM) results in poor survival of gastric cancer (GC) patients. While immunotherapy has emerged as a promising approach for late‐stage GC, validated immune‐related prognostic signatures still remain in need. In this study, we constructed an ovarian metastasis‐ and immune‐related prognostic signature (OMIRPS), characterized the molecular and immune features of OMIRPS‐categorized subgroups and predicted their potential response to immunotherapy.MethodsThree individual cohorts were used to construct and evaluate OMIRPS: RNA‐seq of matched primary GC and OM from Fudan University Shanghai Cancer Center (FUSCC) (discovery cohort, n = 4), The Cancer Genome Atlas (TCGA) (training cohort, n = 544) and GSE84437 (validation cohort, n = 433). Differentially expressed genes (DEGs) identified between primary GC and OM and immune‐related genes (IRGs) from the ImmPort and InnateDB databases were used to identify immune‐related prognostic hub genes, which were further used to construct OMIRPS by using LASSO regression analysis. Prognosis, molecular characteristics, immune features, and differential immunotherapy efficacy between different OMIRPS subgroups were analyzed.ResultsFunctional analyses of DEGs revealed the significance of immune‐related signatures and pathways in the OM. Immune‐related prognostic hub genes including TNFRSF18, CARD11, BCL11B, NRP1, BNIP3L, and ATF3 were utilized to construct OMIRPS, which was identified as an independent prognostic factor. Comprehensive analyses unveiled the distinctive molecular and immune characteristics of OMIRPS‐high and ‐low subgroup in regard to enriched pathways, mutation rate, tumor mutation burden, microsatellite instability status, infiltrated immune cell, immune exclusion score, and the prediction of immunotherapy efficacy. Additionally, OMIRPS was associated with Immune Subtypes with borderline significance.ConclusionsRNA‐seq of paired primary and ovarian metastatic tumors unveiled the significance of immune‐related pathways and tumor immune microenvironment in OM. OMIRPS served as a promising biomarker to predict the prognosis of GC patients and distinguish the molecular features, immune characteristics, and efficacy of immunotherapy between different subgroups.
A real‐world comparison between neoadjuvant chemoimmunotherapy and chemotherapy alone for resectable non‐small cell lung cancer
Authors: Zhang Baihua , Xiao Haifan , Pu Xingxiang , Zhou Chunhua , Yang Desong , Li Xu , Wang Wenxiang , Xiao Qin 
Published: May 2022

AbstractBackgroundThe impact of neoadjuvant chemoimmunotherapy on pulmonary resection and related outcomes had been poorly reported in previous studies. The present study aims to clarify the efficacy and safety of neoadjuvant chemoimmunotherapy, and intraoperative difficulty in the following surgery, in comparison with chemotherapy alone in non‐small cell lung cancer (NSCLC).MethodsPatients with newly diagnosed clinical stages IB–IIIB(T3‐4N2) NSCLC, received neoadjuvant chemotherapy + PD‐1 inhibitors (PD‐1 + Chemo group) or chemotherapy alone (Chemo group) followed by surgery between December 2018 and December 2020 were included. The clinicopathological characteristics were retrospectively reviewed and analyzed.ResultsThere were 69 NSCLC patients in the PD‐1 + Chemo group and 121 in the Chemo group. The major pathological response (MPR) rate in the PD‐1 + Chemo group was 49.3%, higher than that of 19.0% in the Chemo group (p < 0.001). The 2‐year disease‐free survival (DFS) rate was 79.3% and 60.2%, respectively, in the two groups (p = 0.048). Multivariate analysis identified surgical radicality (hazard ratio (HR), 2.954, 95% confidence interval (CI), 1.527–5.714, p = 0.001), and pathological response (MPR(CR) vs. SD(PD), HR, 0.248, 95% CI, 0.107–0.572, p = 0.001) to be independent prognostic factors for DFS. Lobectomy was performed in 73.9% and 66.1% of patients, respectively, and bronchial sleeve resection/bronchoplasty rate was also comparable (43.4% vs. 40.5%, p = 0.688). More patients in the PD‐1 + Chemo group received vascular sleeve resection/angioplasty (15.9% vs. 6.6%, p = 0.039) and pericardial resection (10.1% vs. 2.5%, p = 0.038). After propensity score matching analysis, pericardial resection rate was still slightly higher in the PD‐1 + Chemo group (9.4% vs. 1.6%, p = 0.05). Perioperative morbidities within 30 days and mortality in 90 days were comparable between groups (p > 0.05).ConclusionsNeoadjuvant chemoimmunotherapy for NSCLC is safe and feasible, with higher MPR rates, as well as favorable DFS than chemotherapy alone. Surgical complexity might be increased in certain patients, with comparable perioperative morbidity and mortality.
Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones
Authors: Milutinović Violeta M. , Matić Ivana Z. , Stanojković Tatjana P. , Soković Marina D. , Ćirić Ana D. , Ušjak Ljuboš J. , Niketić Marjan S. , Petrović Silvana D. 
Published: May 2022

AbstractAntimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8‐epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8‐epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC‐5 cells.
Two New Xanthones from the Twigs of Calophyllum membranaceum and Their Anti‐Inflammatory Activities in HESC Cells
Authors: Li Si‐Yuan , Mi Ya‐Hui , Shen Wei , Hu Xiao‐Long , Zhang Jing , Nan Fang , Song Zhe , Zhang Xiao‐Qi , Ye Wen‐Cai , Wang Hao 
Published: May 2022

AbstractTwo new xanthones, calmemxanthone A (1) and calmemxanthone B (2), along with eleven known compounds were isolated from the dried twigs of Calophyllum membranaceum Gardn. et Champ. The structures of compounds 1 and 2 were established by analysis of spectra and mass spectrometry data. The absolute configuration of compound 1 was confirmed by electronic circular dichroism (ECD) spectral analysis. The anti‐inflammation action of these compounds were evaluated on lipopolysaccharide (LPS)‐induced inflammatory damage to human endometrial stromal cells (HESCs), and the structure‐activities of 1–13 were also discussed. Compound 10 presented the anti‐inflammation action with an IC50 value of 20.3 μM, that might be relevant to the regulation of NF‐κB signaling pathway via the suppression of TRIF, IKKα, and IκBα.
Safety and efficacy of 48 mm Xience Xpedition everolimus‐eluting stent for the treatment of long coronary lesions
Authors: Gautier Alexandre , Hovasse Thomas , Arroyo Diego , Unterseeh Thierry , Garot Philippe , Champagne Stéphane , Neylon Antoinette , Sanguineti Francesca , Benamer Hakim , Chevalier Bernard , Lefèvre Thierry 
Published: May 2022

AbstractIntroductionLong drug‐eluting stents may limit the issue of overlapping multiple stents when treating long coronary lesions.AimThe aim of the study was to assess the safety and efficacy of the 48 mm Xience Xpedition everolimus‐eluting stent (48mm‐EES) for the treatment of long coronary lesions, in an all‐comer population.MethodsPatients receiving at least one 48mm‐EES were prospectively included from March 2014 to December 2018. The primary endpoint was target lesion failure (TLF), defined as a composite of cardiac death, target vessel myocardial infarction, and clinically driven target lesion revascularization (TLR) at 1 year. The main secondary endpoint was the patient‐oriented composite endpoint (POCE) defined as a composite of death, stroke, myocardial infarction, and reintervention.ResultsA total of 268 patients with 276 long coronary lesions, including 94 chronic total occlusions (CTO), were successfully treated using at least one 48mm‐EES. The total stent length per lesion was 66 ± 22 mm. A single 48mm‐EES was suitable to successfully treat the target lesion in 48% of cases (60% for non‐CTO lesions). One‐year follow‐up rate was 96.3%. TLF occurred in 13 patients (5.3%), mainly driven by TLR (4.1%). Two cardiac death occurred (0.7%). POCE occurred in 30 patients (11.6%) mainly driven by repeat revascularization (9.7%). Definite stent thrombosis was observed in two patients (0.7%). No difference was observed in one‐year outcomes between single 48mm‐EES and multiple stents implantation as well as between CTO and non‐CTO lesions.ConclusionThe 48mm‐EES is safe and effective to treat long coronary lesions, including CTOs, and provides attractive cost‐effectiveness by limiting multiple stenting.
Isolated psoas abscess caused by Mycobacterium tuberculosis: A rare case report
Authors: Vasigh Mahtab , Karoobi Mohammadreza , Montazeri Mahnaz , Moradi Golnaz , Asefi Hoda , Gilani Abolfazl , Meshkati yazd Seyed mostafa 
Published: May 2022

AbstractPsoas tuberculosis abscess is very rarely detected primarily without an adjacent vertebral cold abscess. Early diagnosis prevents unnecessary operations and life‐threatening complications.
Successful repair of a postcardiac surgery ascending aortic pseudoaneurysm
Authors: Contrafouris Constantinos , Samanidis George , Katsilouli Spyridoula , Nenekidis Ioannis , Roussakis Antonios , Perreas Konstantinos , Chatzis Andrew 
Published: May 2022

AbstractWe present the case of a 67‐year‐old asymptomatic man with a history of coronary artery bypass surgery and a pseudoaneurysm of the ascending aorta treated with a low‐risk alternate procedure. At 1‐year follow‐up, the cardiac computed tomography was not detected residual aneurysm in ascending aorta.
Horizontal stenting via retrograde route for recurrent ruptured posterior communicating artery aneurysm after clipping: A case report and literature review
Authors: Fuga Michiyasu , Tanaka Toshihide , Tachi Rintaro , Nogami Ryo , Teshigawara Akihiko , Ishibashi Toshihiro , Hasegawa Yuzuru , Murayama Yuichi 
Published: May 2022

AbstractTreatment of recurrent ruptured aneurysms incorporating a branch vessel arising from the dome is challenging. Here, we attempted horizontal stent‐assisted coil embolization via a retrograde route from the contralateral internal carotid artery to treat a small ruptured posterior communicating artery aneurysm incorporating a fetal variant posterior cerebral artery after clipping.
E. coli bacterial meningitis after transrectal prostate biopsy under antibiotic prophylaxis: a case report and literature review
Authors: Vandewalle Tom , Vander Eeckt Kathy , Deconinck Steven , Weyne Emmanuel , Verpoort Pieter , Ost Dieter 
Published: May 2022

AbstractProstate biopsy, a frequently performed procedure, is not harmless. In rare cases, life‐threatening complications occur. We document a potential lethal bacterial meningitis after transrectal biopsy. In addition to our overview of all previously documented cases, we highlight the evidence of prevention of infectious complications when performing a prostate biopsy.
Constructing Ion‐Selective Coating Layer with Lithium Ion Conductor LLZO and Binder Li‐Nafion for Separator Used in Lithium‐Sulfur Batteries
Authors: Huang Boyang , Hua Haiming , Lai Pengbin , Shen Xiu , Li Ruiyang , He Zheng , Zhang Peng , Zhao Jinbao 
Published: May 2022

AbstractIn the study of lithium‐sulfur batteries, the shuttle effect of polysulfide ions is widely regarded as the most critical factor in the battery performance deterioration. In this article, with the strategy of ion‐selection, a method of preparing a coating layer with solid‐phase conduction for a separator was used to suppress such an effect. The coating layer was based on calcined ceramic Li7La3Zr2O12 (LLZO) and formed through a simple coating method to be thin but dense. The gap between particles was filled with lithiated Nafion (Li‐Nafion) which could block polysulfide ions through coulombic interactions of sulfonate groups and allowed the lithium ion conduction. The properties of compactness and the ion‐selection formed a double barrier effect, manifested as the fact that the estimated lithium ion transport number achieved 0.79. Correspondingly, the lithium‐sulfur batteries assembled with the ion‐selective conduction separators had better lithium deposition behavior and exhibit brilliant cycling performances and capacity retention.
Selective Detection of Mg2+ for Sensing Applications in Drinking Water
Authors: Paderni Daniele , Macedi Eleonora , Lvova Larisa , Ambrosi Gianluca , Formica Mauro , Giorgi Luca , Paolesse Roberto , Fusi Vieri 
Published: May 2022

AbstractA new series of ligands containing the 2‐(2‐hydroxy‐3‐ naphthyl)‐4‐methylbenzoxazole (HNBO) fluorophore showed selectivity for Mg2+ ions, without the interference of Ca2+. The most promising representative L3 resulted the best performing sensor for Mg2+ both in solution and embedded in an all‐solid‐state optode, especially towards real samples of drinkable water.
Solvent‐Free Synthesis of Core‐Functionalised Naphthalene Diimides by Using a Vibratory Ball Mill: Suzuki, Sonogashira and Buchwald–Hartwig Reactions
Authors: Panther Lydia A. , Guest Daniel P. , McGown Andrew , Emerit Hugo , Tareque Raysa Khan , Jose Arathy , Dadswell Chris M. , Coles Simon J. , Tizzard Graham J. , González‐Méndez Ramón , Goodall Charles A. I. , Bagley Mark C. , Spencer John , Greenland Barnaby W. 
Published: May 2022

AbstractSolvent‐free synthesis by using a vibratory ball mill (VBM) offers the chance to access new chemical reactivity, whilst reducing solvent waste and minimising reaction times. Herein, we report the core functionalisation of N,N’‐bis(2‐ethylhexyl)‐2,6‐dibromo‐1,4,5,8‐naphthalenetetracarboxylic acid (Br2‐NDI) by using Suzuki, Sonogashira and Buchwald–Hartwig coupling reactions. The products of these reactions are important building blocks in many areas of organic electronics including organic light‐emitting diodes (OLEDs), organic field‐effect transistors (OFETs) and organic photovoltaic cells (OPVCs). The reactions proceed in as little as 1 h, use commercially available palladium sources (frequently Pd(OAc)2) and are tolerant to air and atmospheric moisture. Furthermore, the real‐world potential of this green VBM protocol is demonstrated by the double Suzuki coupling of a monobromo(NDI) residue to a bis(thiophene) pinacol ester. The resulting dimeric NDI species has been demonstrated to behave as an electron acceptor in functioning OPVCs.
Azole‐Directed Cobalt‐Catalyzed Asymmetric Hydrogenation of Alkenes
Authors: Jin Yue , Zou Yashi , Hu Yanhua , Han Yunxi , Zhang Zhenfeng , Zhang Wanbin 
Published: May 2022

AbstractThe azole‐directed cobalt‐catalyzed asymmetric hydrogenation of alkenes has been developed with high efficiency. With this approach, chiral pyrazole compounds were obtained in quantitative yields and excellent enantioselectivities (up to 99 % ee) under mild conditions, and the hydrogenation was conducted on a gram scale with up to 2000 TON. Several useful applications were demonstrated including the convenient introduction of β‐chirality to a drug intermediate containing an azole ring.
Automated Knowledge‐Based Design for Additive Manufacturing: A Case Study with Flow Manifolds
Authors: Biedermann Manuel , Beutler Patrick , Meboldt Mirko 
Published: May 2022

AbstractAdditive manufacturing (AM) enables novel process equipment devices. However, the manual 3D design of complex part geometries is frequently challenging as designers need to fulfill functional requirements and consider the restrictions of AM. One promising approach is to automatically generate the design of parts. This work aims to introduce such an automated design approach to practitioners in the area of process engineering. The work describes the basic steps required to develop an automated, knowledge‐based design tool for AM and demonstrates the approach using a case study on flow manifolds.
Advances in the Analysis of Pharmaceuticals by Using Graphene‐Based Sensors
Authors: Lai Wing‐Fu , Reddy Obireddy Sreekanth , Zhang Haotian , Zhang Dahong , Wong Wing‐Tak 
Published: May 2022

AbstractSafe and effective use of drugs relies on proper pharmaceutical analysis. Graphene has been extensively used to construct sensors for this purpose. Over the years, a large variety of pharmaceutical sensors have been developed from graphene or its derivatives. This article reviews the current status of sensor development from graphene and its derivatives, and discusses the use of graphene‐based sensors in pharmaceutical analysis. It is hoped that this article offers not only a snapshot of recent advances in the fabrication and use of graphene‐based sensors, but also provides insights into future engineering and optimization of the sensors for effective pharmaceutical analysis.
The effect of different cleansing methods for removing temporary cement on the tensile bond force of permanently cemented implant‐supported metal copings: An in vitro study
Authors: Ebadian Behnaz , Jowkar Mohammad , Davoudi Amin , Fathi Amirhossein , Ziaei Mohsen , Berg Einar 
Published: May 2022

AbstractObjectivesComplete cleaning of temporary cement before permanent cementation of cement‐retained implant‐supported prosthesis (CISP) when recementing the crown is critical. This study evaluated the effect of different cleaning methods for removing traces of temporary cement on the final tensile bond force (TBF) of CISP recemented with resin cement.Materials and MethodsSeventy computer‐aided design/computer‐aided manufacturing metal implant‐supported copings were prepared and distributed into seven groups (N = 10). Copings of six groups (60 samples) were cemented with temporary cement with eugenol and subjected to 5000 thermocycling. After debonding by a universal testing machine, the internal surfaces of the copings were cleaned using one of the six following methods: 1‐an ultrasonic water bath (UW), 2‐sandblasting, then washing with water (SW), 3‐sandblasting and an ultrasonic water bath (SUW), 4‐an ultrasonic isopropyl alcohol bath (UA), 5‐sandblasting, then washing with isopropyl alcohol (SA) or 6‐sandblasting and an ultrasonic isopropyl alcohol bath (SUA). Then the subjects were subsequently cemented by dual‐cure self‐adhesive resin cement. In the seventh group (control, N = 10), the copings were cemented by dual‐cure self‐adhesive resin cement without the temporization phase. The TBF was tested using a universal testing machine with a cross‐head speed of 1 mm/min. Two‐way analysis of variance (ANOVA) and post‐hoc Tamhane tests were used for statistical analysis at a significance level of α = .05.ResultsThe maximum mean of TBF value was observed in SUA group (845 ± 203 N), and the minimum was observed in the temporary cement group (49 ± 20 N). All groups which were cleaned with isopropyl alcohol showed significantly higher TBF values compared with those cleaned with water.ConclusionsCleaning of the inner surface of metal copings after debonding with sandblasting and isopropyl alcohol results in the highest value of TBF by eliminating the effect of remaining eugenol and removing traces of temporary cements.