bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hyperbaric oxygen therapy improves neurocognitive functions and symptoms of post-COVID condition: randomized controlled trial
Authors: Zilberman-Itskovich Shani , Catalogna Merav , Sasson Efrat , Elman-Shina Karin , Hadanny Amir , Lang Erez , Finci Shachar , Polak Nir , Fishlev Gregory , Korin Calanit , Shorer Ran , Parag Yoav , Sova Marina , Efrati Shai 
Published: July 2022

AbstractPost-COVID-19 condition refers to a range of persisting physical, neurocognitive, and neuropsychological symptoms after SARS-CoV-2 infection. The mechanism can be related to brain tissue pathology caused by virus invasion or indirectly by neuroinflammation and hypercoagulability. This randomized, sham-control, double blind trial evaluated the effect of hyperbaric oxygen therapy (HBOT or HBO2 therapy) on post-COVID-19 patients with ongoing symptoms for at least 3 months after confirmed infection. Seventy-three patients were randomized to receive daily 40 session of HBOT (n = 37) or sham (n = 36). Follow-up assessments were performed at baseline and 1–3 weeks after the last treatment session. Following HBOT, there was a significant group-by-time interaction in global cognitive function, attention and executive function (d = 0.495, p = 0.038; d = 0.477, p = 0.04 and d = 0.463, p = 0.05 respectively). Significant improvement was also demonstrated in the energy domain (d = 0.522, p = 0.029), sleep (d = − 0.48, p = 0.042), psychiatric symptoms (d = 0.636, p = 0.008), and pain interference (d = 0.737, p = 0.001). Clinical outcomes were associated with significant improvement in brain MRI perfusion and microstructural changes in the supramarginal gyrus, left supplementary motor area, right insula, left frontal precentral gyrus, right middle frontal gyrus, and superior corona radiate. These results indicate that HBOT can induce neuroplasticity and improve cognitive, psychiatric, fatigue, sleep and pain symptoms of patients suffering from post-COVID-19 condition. HBOT’s beneficial effect may be attributed to increased brain perfusion and neuroplasticity in regions associated with cognitive and emotional roles.
Evolutionary ecology of Miocene hominoid primates in Southeast Asia
Authors: Habinger S. G. , Chavasseau O. , Jaeger J.-J. , Chaimanee Y. , Soe A. N. , Sein C. , Bocherens H. 
Published: July 2022

AbstractThe evolutionary history and palaeoecology of orangutans remains poorly understood until today. The restricted geographic distribution of extant Pongo indicates specific ecological needs. However, it is not clear whether these needs were shared by the great diversity of fossil pongines known from the Miocene to the Pleistocene. Here we show how niche modelling of stable carbon and oxygen isotope data of the carbonate fraction of dental enamel can be used to reconstruct the paleoecology of fossil and modern pongines and associated mammal communities. We focus on Khoratpithecus ayeyarwadyensis, a Late Miocene pongine from Myanmar and the sister clade to extant orangutans, and compare it to its associated mammal fauna and other fossil and extant pongines. The results are consistent with a vertical position high up in the canopy of a forested habitat with purely C3 vegetation for K. ayeyarwadyensis as well as the contemporaneous Sivapithecus. Although their positions in the modelled isotopic niche space look similar to the ecological niche occupied by modern Pongo, a comparison of the modelled niches within the pongine clade revealed possible differences in the use of microhabitats by the Miocene apes.
Photothermal effect of albumin-modified gold nanorods diminished neuroblastoma cancer stem cells dynamic growth by modulating autophagy
Authors: Shahabad Zahra Alizadeh , Avci Cigir Biray , Bani Farhad , Zarebkohan Amir , Sadeghizadeh Majid , Salehi Roya , Ghafarkhani Maryam , Rahbarghazi Reza , Bagca Bakiye Goker , Ozates Neslihan Pınar 
Published: July 2022

AbstractHere, we investigated the photothermal effect of gold nanorods (GNRs) on human neuroblastoma CD133+ cancer stem cells (CSCs) via autophagic cell death. GNRs were synthesized using Cetyltrimethylammonium bromide (CTAB), covered with bovine serum albumin (BSA). CD133+ CSCs were enriched from human neuroblastoma using the magnetic-activated cell sorting (MACS) technique. Cells were incubated with GNRs coated with BSA and exposed to 808-nm near-infrared laser irradiation for 8 min to yield low (43 °C), medium (46 °C), and high (49 °C) temperatures. After 24 h, the survival rate and the percent of apoptotic and necrotic CSCs were measured using MTT assay and flow cytometry. The expression of different autophagy-related genes was measured using polymerase chain reaction (PCR) array analysis. Protein levels of P62 and LC3 were detected using an enzyme-linked immunosorbent assay (ELISA). The viability of CSC was reduced in GNR-exposed cells compared to the control group (p < 0.05). At higher temperatures (49 °C), the percent of apoptotic CSCs, but not necrotic cells, increased compared to the lower temperatures. Levels of intracellular LC3 and P62 were reduced and increased respectively when the temperature increased to 49 °C (p < 0.05). These effects were non-significant at low and medium temperatures (43 and 46 °C) related to the control CSCs (p > 0.05). The clonogenic capacity of CSC was also inhibited after photothermal therapy (p < 0.05). Despite these changes, no statistically significant differences were found in terms of CSC colony number at different temperatures regardless of the presence or absence of HCQ. Based on the data, the combination of photothermal therapy with HCQ at 49 °C can significantly abort the CSC clonogenic capacity compared to the control-matched group without HCQ (p < 0.0001). PCR array showed photothermal modulation of CSCs led to alteration of autophagy-related genes and promotion of co-regulator of apoptosis and autophagy signaling pathways. Factors related to autophagic vacuole formation and intracellular transport were significantly induced at a temperature of 49 °C (p < 0.05). We also note the expression of common genes belonging to autophagy and apoptosis signaling pathways at higher temperatures. Data showed tumoricidal effects of laser-irradiated GNRs by the alteration of autophagic response and apoptosis.
Role of birth order, gender, and region in educational attainment in Pakistan
Authors: Raza Syed Hassan , Shah Zulfiqar Ali , Haq Wajiha 
Published: July 2022

AbstractWe studied the effects of birth order and socioeconomic factors on educational attainment in Pakistan. We examined this relationship by using PSLM/HIES 2018–19 which is nationally representative household survey data. We found striking evidence that being born first as a male child is positively and significantly associated with educational attainment. Whereas in our girls' sample we found that being born first is significantly and negatively associated with educational attainment, and this effect does not persist for second-born female children if the firstborn is a male child. Such a difference in our estimates led us to investigate further the cohort and rural–urban dimensions. We concluded that urban areas in Pakistan are primarily responsible for resource rationing in favor of male children for younger cohorts. Therefore, the study recommends the targeted policy intervention to remove such differentials based on gender when it comes to the educational attainment of a child.
Sit to stand muscle power reference values and their association with adverse events in Colombian older adults
Authors: Ramírez-Vélez Robinson , Izquierdo Mikel , García-Hermoso Antonio , Ordoñez-Mora Leidy T. , Cano-Gutierrez Carlos , Campo-Lucumí Florelba , Pérez-Sousa Miguel Ángel 
Published: July 2022

AbstractRecently, a valid method to assess lower-body muscle power based on a sit-to-stand field test (STS) has been published. Our study aimed to describe lower-body muscle power in older individuals aged ≥ 60 years and examine the relationship of muscle weakness with adverse events according to gender- and age-specific muscle weakness cut-off points. A total of 3689 Colombian older adults (57.6% women, age 69.1 ± 6.9 years) from the 2015 Survey on Health, Well-Being, and Aging in Latin America and the Caribbean (SABE) participated in this study. Lower-body muscle power normalized to body mass was estimated by the five-repetitions STS test. Anthropometric, physical performance and clinical characteristics were collected. Age-specific percentiles using the LMS method, cut-off points and association with adverse events were calculated. Lower-body muscle power was greater in men than among women (2.2 ± 0.7 vs. 1.6 ± 0.5 W·kg−1, respectively; p < 0.001) at all ages. Muscle power ranked in the 50th percentile between 2.38 and 1.30 W·kg−1 in men, whereas women ranked between 1.79 and 1.21 W·kg−1. According to the cut-off points, lower-limb muscle power < 1 standard deviation in men was associated with having dynapenia, poor gait speed, cognitive impairment and mental, visual, hearing and memory problems. While, women were associated with having sarcopenia, dynapenia, poor gait speed, cognitive impairment, mental, hearing and memory problems, dementia and hospitalizations of > 24 h in the last year. Overall, participants with poor lower-limb muscle power had a significantly higher risk of adverse events [in men: odds ratio (OR) = 1.51, 95% confidence interval (CI) = 1.19–1.91, p < 0.001; in women: OR = 1.52, 95% CI = 1.27–1.87, p = 0.001] than their stronger counterparts. This study is the first to describe lower-limb muscle power values and cut-off points among a nationally representative sample of Colombian older adults. In men, 7 of the 14 adverse events studied were associated with lower muscle strength, whereas in women, it was 9 of the 14 adverse events.
Purification, characterization, and preliminary serial crystallography diffraction advances structure determination of full-length human particulate guanylyl cyclase A receptor
Authors: Zhang Shangji , Hansen Debra T. , Martin-Garcia Jose M. , Zook James D. , Pan Shuchong , Craciunescu Felicia M. , Burnett John C. , Fromme Petra 
Published: July 2022

AbstractParticulate Guanylyl Cyclase Receptor A (pGC-A) is a natriuretic peptide membrane receptor, playing a vital role in controlling cardiovascular, renal, and endocrine functions. The extracellular domain interacts with natriuretic peptides and triggers the intracellular guanylyl cyclase domain to convert GTP to cGMP. To effectively develop methods to regulate pGC-A, structural information on the full-length form is needed. However, structural data on the transmembrane and intracellular domains are lacking. This work presents expression and optimization using baculovirus, along with the first purification of functional full-length human pGC-A. In vitro assays revealed the pGC-A tetramer was functional in detergent micelle solution. Based on our purification results and previous findings that dimer formation is required for functionality, we propose a tetramer complex model with two functional subunits. Previous research suggested pGC-A signal transduction is an ATP-dependent, two-step mechanism. Our results show the binding ligand also moderately activates pGC-A, and ATP is not crucial for activation of guanylyl cyclase. Furthermore, crystallization of full-length pGC-A was achieved, toward determination of its structure. Needle-shaped crystals with 3 Å diffraction were observed by serial crystallography. This work paves the road for determination of the full-length pGC-A structure and provides new information on the signal transduction mechanism.
Effects of virtual hands and feet on the onset time and duration of illusory body ownership
Authors: Kondo Ryota , Sugimoto Maki 
Published: July 2022

AbstractIn the illusory body ownership, humans feel as if a rubber hand or an avatar in a virtual environment is their own body through visual-tactile synchronization or visual-motor synchronization. Despite the onset time and duration of illusory body ownership has been investigated, it is not clear how the onset time and duration change when a part of the body is missing from the full-body. In this study, we investigated the completeness of the full-body for the illusion onset and duration by comparing the following conditions: complete avatar, avatar missing hands and feet, and avatar with hands and feet only. Our results suggest that avatar hands and feet only shorten the duration of the illusion, and missing body parts, such as only hands and feet or no hands and feet, reduce the sense of body ownership and of agency. However, the effects of avatar completeness on the onset time are unclear, and no conclusions can be made in either direction based on the current findings.
Monitoring of Campylobacter jejuni in a chicken infection model by measuring specific volatile organic compounds and by qPCR
Authors: Hankel Julia , Gibson Timothy , Skov Julia , Andersen Karsten Brandt , Dargatz Michelle , Kappel Andreas , Thiemann Frank , Curtis Ben , Chuppava Bussarakam , Visscher Christian 
Published: July 2022

AbstractCampylobacter is one of the leading bacterial foodborne pathogens worldwide. Poultry is the host species with this pathogen with the highest clinical impact. Flocks become colonised with Campylobacter, which leads to contamination of product entering the food-chain. Rapid and reliable Campylobacter detection methods could support controls to minimize the risks of contamination within the food-chain, which would easier enable the implementation of a logistical slaughter schedule or other control options. The present study evaluates current and emerging C. jejuni detection technologies on air samples in a unique study set-up of pre-defined C. jejuni prevalences. Both non-invasive detection technologies on air samples by subsequent measuring of volatile organic compounds (VOCs) or by qPCR detected the C. jejuni presence and could additionally distinguish between the number of present C. jejuni-positive birds in the study set-up. Nevertheless, electrostatic air samplers diagnosed fewer birds as C. jejuni-positive compared to the cultivation-based method. By measuring the VOCs, it was possible to detect the presence of two positive birds in the room. This apparent high sensitivity still needs to be verified in field studies. Techniques, such as these promising methods, that can facilitate C. jejuni surveillance in poultry flocks are desirable to reduce the risk of infection for humans.
Treadmill locomotion in the American alligator (Alligator mississippiensis) produces dynamic changes in intracranial cerebrospinal fluid pressure
Authors: Young Bruce A. , Cramberg Michael J. 
Published: July 2022

AbstractTo examine the influence of movement on cerebrospinal fluid (CSF) dynamics, intracranial subdural pressure recordings were taken from sub-adult alligators (Alligator mississippiensis) locomoting on a treadmill. Pressure recordings documenting the cardiac, ventilatory, and barostatic influences on the CSF were in good agreement with previous studies. During locomotion the CSF exhibits sinusoidal patterns of pressure change that spanned a mean amplitude of 56 mm Hg, some 16 × the amplitude of the cardiac-linked pulsations. These sinusoidal CSF pulsations were closely linked to the locomotor kinematics, particularly the lateral oscillations of the alligator’s head. Data recorded from the freely moving alligators suggest that fluid inertia, body cavity pressures, and likely other factors all influence the CSF pressure. The clear relationship between movement and CSF pressure described in this study suggests that the paucity of studies examining human CSF dynamics during movement should be addressed.
Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis
Authors: Li Lingzi , Freitag Janina , Asbrand Christian , Munteanu Bogdan , Wang Bei-Tzu , Zezina Ekaterina , Didier Michel , Thill Gilbert , Rocher Corinne , Herrmann Matthias , Biesemann Nadine 
Published: July 2022

AbstractRheumatoid arthritis (RA) is characterized by joint infiltration of immune cells and synovial inflammation which leads to progressive disability. Current treatments improve the disease outcome, but the unmet medical need is still high. New discoveries over the last decade have revealed the major impact of cellular metabolism on immune cell functions. So far, a comprehensive understanding of metabolic changes during disease development, especially in the diseased microenvironment, is still limited. Therefore, we studied the longitudinal metabolic changes during the development of murine arthritis by integrating metabolomics and transcriptomics data. We identified an early change in macrophage pathways which was accompanied by oxidative stress, a drop in NAD+ level and induction of glucose transporters. We discovered inhibition of SIRT1, a NAD-dependent histone deacetylase and confirmed its dysregulation in human macrophages and synovial tissues of RA patients. Mining this database should enable the discovery of novel metabolic targets and therapy opportunities in RA.
Analysis of entropy production in a bi-convective magnetized and radiative hybrid nanofluid flow using temperature-sensitive base fluid (water) properties
Authors: Barman Tapas , Roy S. , Chamkha Ali J. 
Published: July 2022

AbstractThe heat transport characteristics, flow features, and entropy-production of bi-convection buoyancy induced, radiation-assisted hydro-magnetic hybrid nanofluid flow with thermal sink/source effects are inspected in this study. The physical characteristics of hybrid nanofluids (water-hosted) are inherited from the base liquid (water) and none has considered the physical characteristics of base liquid (water) in the study of temperature-sensorial hybrid nanofluid investigations, though the water physical characteristics are not constants in temperature variations. So, the temperature-sensorial attributes of base liquid (water) are taken into account for this hybrid nanofluid ($$Cu+{Al}_{2}{O}_{3}+\text{water}$$

C
u
+

Al

2

O
3

+
water

) flow analysis. The mathematical forms of the flow configuration (i.e., the set of coupled, nonlinear PDE form of governing equations) are solved by utilizing the subsequent tasks: (i) congenial transformation; (ii) quasilinearization; (iii) methods of finite differences to form block matrix system, and (iv) Varga’s iterative algorithm. The preciseness of the whole numerical procedure is ensured by restricting the computation to follow strict convergence conditions. Finally, the numerically extracted results representing the impacts of various salient parameters on different profiles ($$F, G, H$$

F
,
G
,
H

), gradients, and entropy production are exhibited in physical figures for better perception. A few noticeable results are highlighted as: velocity graph shows contrast behaviour under assisting and opposing buoyancy; temperature ($$G(\xi ,\eta )$$

G
(
ξ
,
η
)

) is dropping for heightening heat source ($$Q$$
Q
) surface friction remarkably declines with the outlying magnetic field ($$St$$

St

); thermal transport confronts drastic abatement under radiation ($${R}_{1}$$

R
1

), and $$St$$

St

; the characteristics Reynolds and Brinkman numbers promote entropy. Furthermore, the bounding surface acts as a strong source of $${S}_{G}$$

S
G

-production. Summarizations are listed at the end to quantify percentage variations.
A machine learning model for predicting surgical intervention in renal colic due to ureteral stone(s) < 5 mm
Authors: Haifler Miki , Kleinmann Nir , Haramaty Rennen , Zilberman Dorit E. 
Published: July 2022

AbstractA 75–89% expulsion rate is reported for ureteric stones ≤ 5 mm. We explored which parameters predict justified surgical intervention in cases of pain caused by < 5 mm ureteral stones. We retrospectively reviewed all patients with renal colic caused by ureteral stone < 5 mm admitted to our urology department between 2016 and 2021. Data on age, sex, body mass index, the presence of associated hydronephrosis/stranding on images, ureteral side, stone location, medical history, serum blood count, creatinine, C-reactive protein, and vital signs were obtained upon admission. XGboost (XG), a machine learning model has been implemented to predict the need for intervention. A total of 471 patients (median age 49, 83% males) were reviewed. 74% of the stones were located in the distal ureter. 160 (34%) patients who sustained persistent pain underwent surgical intervention. The operated patients had proximal stone location (56% vs. 10%,p < 0.001) larger stones (4 mm vs. 3 mm,p < 0.001), longer length of stay (3.5 vs. 3 days,p < 0.001) and more emergency-room (ER) visits prior to index admission (2 vs. 1,p = 0.007) compared to those who had no surgical intervention. The model accuracy was 0.8. Larger stone size and proximal location were the most important features in predicting the need for intervention. Altogether with pulse and ER visits, they contributed 73% of the final prediction for each patient. Although a high expulsion rate is expected for ureteral stones < 5 mm, some may be painful and drawn out in spontaneous passage. Decision-making for surgical intervention can be facilitated by the use of the present prediction model.
Development of a polyacrylamide/chitosan composite hydrogel conduit containing synergistic cues of elasticity and topographies for promoting peripheral nerve regeneration
Authors: Liu Fang , Xu Jiawei , Liu Anning , Wu Linliang , Wang Dongzhi , Han Qi , Zheng Tiantian , Wang Feiran , Kong Yan , Li Guicai , Li Peng , Gu Shouyong , Yang Yumin 
Published: July 2022

The polyacrylamide/chitosan composite hydrogel containing synergistic cues of elasticity and topographies was developed by in situ free radical polymerization and micromolding methods.
Impaired Categorical Perception of Speech Sounds Under the Backward Masking Condition in Adults Who Stutter
Authors: Shao Jing , Bakhtiar Mehdi , Zhang Caicai 
Published: July 2022

Purpose:
Evidence increasingly indicates that people with developmental stuttering have auditory perception deficits. Our previous research has indicated similar but slower performance in categorical perception of the speech sounds under the quiet condition in children who stutter and adults who stutter (AWS) compared with their typically fluent counterparts. We hypothesized that the quiet condition may not be sufficiently sensitive to reveal subtle perceptual deficiencies in people who stutter. This study examined this hypothesis by testing the categorical perception of speech and nonspeech sounds under backward masking condition (i.e., a noise was presented immediately after the target stimuli).

Method:
Fifteen Cantonese-speaking AWS and 15 adults who do not stutter (AWNS) were tested on the categorical perception of four stimulus continua, namely, consonant varying in voice onset time (VOT), vowel, lexical tone, and nonspeech, under the backward masking condition using identification and discrimination tasks.

Results:
AWS demonstrated a broader boundary width than AWNS in the identification task. AWS also exhibited a worse performance than AWNS in the discrimination of between-category stimuli but a comparable performance in the discrimination of within-category stimuli, indicating reduced sensitivity to sounds that belonged to different phonemic categories among AWS. Moreover, AWS showed similar patterns of impaired categorical perception across the four stimulus types, although the boundary location on the VOT continuum occurred at an earlier point in AWS than in AWNS.

Conclusions:
The findings provide robust evidence that AWS exhibit impaired categorical perception of speech and nonspeech sounds under the backward masking condition. Temporal processing (i.e., VOT manipulation), frequency/spectral/formant processing (i.e., lexical tone or vowel manipulations), and nonlinguistic pitch processing were all found to be impaired in AWS. Altogether, the findings support the hypothesis that AWS might be less efficient in accessing the phonemic representations when exposed to a demanding listening condition.

Supplemental Material:

https://doi.org/10.23641/asha.20249718