bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical significance of down-regulated LPCAT1 expression in acute myeloid leukemia
Authors: Chen Qin , Xu Zijun , Lin Jiang , Deng Zhaoqun , Qian Jun , Qian Wei 
Published: August 2022

Abstract
Background Lysophosphatidylcholine acyltransferase 1 (LPCAT1) overexpression has been found in various solid cancers and promoted the progression, metastasis, and recurrence of these diseases. However, the expression pattern of LPCAT1 in acute myeloid leukemia was unclear. This study aimed to assess expression status of LPCAT1 and its clinical relevance in acute myeloid leukemia (AML). Methods and Results LPCAT1 expression was significant lower in AML than controls predicted by public databases. Also, a significantly down-regulated of LPCAT1 of bone marrow in AML compared with controls was validated by real-time quantitative PCR (RQ-PCR) [0.056 (0.000-0.846) vs 0.253 (0.031-1.000)]. The DiseaseMeth version 2.0 and The Cancer Genome Atlas analysis showed LPCAT1 promoter was hypermethylated in AML and a strongly negative correlation was found between LPCAT1 expression and methylation (R=-0.610, P<0.001). RQ-PCR revealed the frequency of LPCAT1 low-expression in FAB-M4/M5 subtype was lower than other subtypes (P=0.018). The ROC curve revealed LPCAT1 expression might serve as a potential diagnostic marker for differentiating AML from normal with an area under the ROC curve of 0.819 (95% CI 0.743-0.894, P<0.001). In cytogenetically normal AML, the overall survival in patients with LPCAT1 low-expression was significantly longer than that in those without LPCAT1 low-expressed group (median 19 versus 5.5 months, P=0.036). Conclusion LPCAT1 is downregulated in bone marrow and LPCAT1 expression can be as a potential biomarker for diagnosis and evaluating prognosis in AML.
Influence of prognostic nutritional index and controlling nutritional status on the prognosis of patients with acute traumatic spinal cord injury
Authors: Wang Zhi-Qiang , Lin LU , Zhong Dian , Ke Zhen-Yong , Cheng Si 
Published: August 2022

Abstract
Objectives: The aim of this study was to define the risk factors affecting the prognosis of patients with acute traumatic spinal cord injury (tSCI), and to discuss the value of prognostic nutritional index (PNI) and controlling nutritional status (COUNT) score in predicting the prognosis of patients with acute tSCI.Methods: We retrospectively analyzed the clinical data of 75 patients with acute tSCI. The optimal cutoff value was obtained through the receiver operating characteristic curve (ROC), and the value of PNI and COUNT score were 45.05 and 3.5 respectively. Patients were divided into high PNI groups and low PNI groups, high COUNT groups and low COUNT groups respectively based on cutoff values. The differences of outcome indexes such as American spinal injury association impairment scale (AIS), exercise score, Bathel index score and light touch score were analyzed, and the prognostic factors of tSCI patients were analyzed. A improved outcome was defined as improvement of AIS grade ≥ 1.Results: Compared with the improvement group, the non-improvement group had lower serum albumin and PNI, higher COUNT score, longer hospital stay and higher postoperative infection rate (P < 0.05). PNI and COUNT score were closely related to the AIS grade (r = 0.629, P < 0.001; r = -0.855, P < 0.001). After adjusting for confounding factors, the odds ratios of PNI and COUNT score for predicting improved outcomes in patients with acute tSCI were 1.396 (95%CI: 1.141-1.709） and 0.284 (95%CI: 0.136-0.0.594）, respectively. The area under the curve (AUC) of PNI and count scores for predicting improved outcomes were 0.752 (95% CI: 0.641 ~ 0.862, P < 0.001) and 0.766 (95% CI: 0.654 ~ 0.879, P < 0.001), respectively. Conclusion: PNI and count scores may be independent predictors of improved outcomes in patients with acute tSCI.
Desorption and degradation of fenobucarb in paddy soil with the presence of pH, dissolved organic carbon, and sodium dodecyl sulphate in irrigation water
Authors: Trinh Ha Thu , Le Giang Truong , Dao Yen Hai , Duong Hanh Thi , Dang Mai Thi , Strobel Bjarne W. 
Published: August 2022

Abstract
The presence of pH, dissolved organic carbon (DOC), and sodium dodecyl sulphate (SDS) in irrigation water for rice fields enhanced the release and biodegradation of fenobucarb in the soil-water system, contributing to the diminishing of fenobucarb and 2-sec-butylphenol in the soil and drain water. The fenobucarb-spiked soil was amended with pH 7, pH 9, 25 mg L− 1 DOC, and 1 critical micelle (2.4 g L− 1) SDS solutions, and the residual fenobucarb and 2-sec-butyl phenol were analysed in water and soil after each decomposition period. The disappearance of fenobucarb and the appearance of 2-sec-butylphenol were the result of biodegradation and hydrolysis, and these processes were influenced by the presence in the order of pH 9, DOC, pH 7, and SDS. In the presence of SDS in the solution, the desorption and biodegradation processes simultaneously affected the degradation of fenobucarb in the soil. However, fenobucarb biodegradation was inhibited, probably because of the preferential utilisation of SDS by fenobucarb degraders.
The effect of connective tissue graft or a collagen matrix on epithelial differentiation around teeth and dental implants: A preclinical study in minipigs
Authors: Stähli Alexandra , Párkányi László , Aroca Sofia , Stavropoulos Andreas , Schwarz Frank , Sculean Anton , Bosshardt Dieter D. 
Published: August 2022

Abstract
Objectives: To histologically evaluate the healing of coronally advanced flap (CAF) with either a superficial (SCTG) or deep palatal connective tissue graft (DCTG), or a collagen matrix (CM) to cover recession defects at teeth and implants.Material and Methods: One mandibular side of 6 miniature pigs received each 3 titanium implants 12 weeks after extraction. Eight weeks later, recession defects were created around implants and contralateral premolars and 4 weeks later randomly subjected to CAF+SCTG, CAF+DCTG, or CAF+CM. After 8 weeks block biopsies were histologically analyzed. Results: Pocket formation was seen at all teeth, around most implants with SCTG and DCTG, however not in the CM implant group. The connective tissue grafts showed hardly signs of degradation, whereas the CM was partly degraded and integrated in connective tissue. All teeth and implants exhibited a keratinized epithelium with no differences among the groups. The mean gain in gingival height was similar in all experimental groups (SCTG 3.89 ± 0.80 mm, DCTG 4.01 ± 1.40 mm, CM 4.21 ± 0.64 mm). Statistically significant differences were found in the height of the junctional epithelium between the control teeth and the connective tissue groups (p=0.009 and 0.044). Conclusions: In terms of keratinization no difference was observed between superficial and deep connective tissue grafts. Using a SCTG or DCTG (not CM) at teeth and implants showed pocket formation with subgingival calculus and inflammatory processes. Generally, CAF+SCTG/DCTG/CM resulted in a long JE that was even longer at implants.Clinical relevance: Deep/superficial palatal connective tissue graft yielded similar keratinization around teeth/implants.
Calreticulin promotes EMT in pancreatic cancer by enhancing endoplasmic reticulum stress-induced autophagy
Authors: Wu Hongjia , Tang Jingtong , Sun Jian , Tian Chen , Li Xin , Zhong Banghua , Ilmer Matthias , Wang Zixin , Dong Ming , Sheng Weiwei 
Published: August 2022

Abstract
Background
Our previous study showed that Calreticulin (CRT) promoted EMT in pancreatic cancer (PC) via mediating endoplasmic reticulum stress (ERS).
Methods
In current study, we further investigate the association of CRT with ERS-induced autophagy in regulating malignant behavior of PC in vitro and vivo.
Results
We first found thapsigargin (TG)-stimulated ERS and subsequent UPR signaling specifically induced the late stage of autophagy in vitro following the activation of ATG5/ATG12/LC3II signaling, and the increase of autophagosome formation and autophagic flux. However, CRISPR/Cas9 mediated CRT silencing reversed ERS-induced autophagy via specifically inhibiting TG-stimulated PERK/eIF2a axis. Similarly, TG-stimulated ERS promoted cell mobility and Gemcitabine resistance in vitro via promoting autophagy, which was significantly reversed by CRT silencing and autophagy inhibitor Chloroquine (CQ). In vivo, CRT silencing and CQ treatment profoundly inhibited TG-induced pancreatic tumor size in situ and the number of distant liver metastasis following the same change of UPR and autophagy signaling as shown in vitro. Mechanistically, CRT was co-localized and co-immunoprecipitated with LC3 under TG treatment. GST pulldown showed a conserved but critical LC3-interacting region (LIR: WDFL) for the interaction between CRT and LC3, which is required for CRT-mediated augmentation of ERS-induced autophagy. The subsequent augmentation of ERS-induced autophagy profoundly promoted EMT in vitro, which was reversed by CRT silencing and CQ. Finally, a close relationship between CRT with critical markers of UPR and autophagy signaling was also observed in clinical PC samples, which coordinately promoted poor prognosis of PC patients.
Conclusions
CRT promotes EMT in PC via enhancing ERS-induced autophagy.
Compressive Property and Nano-mechanical Behavior of Aluminium Cenosphere Composite Foam Developed by Powder Metallurgy Route
Authors: Shukla Amarish Kumar , Mandal Dehipada , Majumdar jyotsna Dutta 
Published: August 2022

Abstract
In the present study, the mechanical properties in terms of nano and microhardness and compressive strength of aluminium cenosphere composite foam (CF) developed by powder metallurgy route was carried out. The CF was developed under a varied compaction pressure between 125 MPa and 375 MPa, and sintered by two step sintering (step 1: heating at 400 ºC for 1 hrs. and step 2: heating at 700ºC for 2 hrs.) with a cenosphere content varying from 5 vol.% to 50 vol.%. The effect of process parameters and cenosphere content on microstructure, porosity and its distribution, microhardness and nano mechanical properties was undertaken. Finally, the compressive behavior of the Al-cenosphere composite foam processed under varied compaction pressure and with varied cenosphere content was evaluated.
Changes in the tumor oxygenation but not in the tumor volume and tumor vascularization reflect early response of breast cancer to neoadjuvant chemotherapy
Authors: Pavlov Mikhail V. , Bavrina Anna P. , Plekhanov Vladimir I. , Golubyatnikov German Yu. , Orlova Anna G. , Subochev Pavel V. , Davydova Diana A. , Turchin Ilya V. , Maslennikova Anna V. 
Published: August 2022

Abstract
Background
Breast cancer neoadjuvant chemotherapy (NACT) allows assessing tumor sensitivity to systemic treatment, planning adjuvant treatment and follow-up. However, a sufficiently large number of patients fail to achieve the desired level of pathological tumor response while optimal early response assessment methods have not been established now. In our study, we simultaneously assessed the early chemotherapy-induced changes in the tumor volume by Ultrasound (US), the tumor oxygenation by Diffuse Optical Spectroscopy Imaging (DOSI) and the state of the tumor vascular bed by Doppler US to elaborate the predictive criteria of breast tumor response to treatment.
Methods
A total of 133 patients with a confirmed diagnosis of invasive breast cancer stage II to III admitted to NACT with following definitive breast surgery were enrolled, 108 of them completed the therapy. Tumor oxygenation by DOSI, tumor volume by US and tumor vascularization by Doppler US were determined before the first and before the second cycle of NACT. After NACT completion, patients underwent surgery followed by pathological examination and assessment of the pathological tumor response. On the basis of these data regression predictive models were created.
Results
We observed changes in all three parameters at the early time after the start of the treatment. However, a high predictive potential for early assessment of tumor sensitivity to NACT demonstrated only the level of oxygenation, ΔStO2, (ρ = 0,802, р≤0,01). The regression model predicts the tumor response with a high probability of a correct conclusion (89,3%). The “Tumor volume” model and the “Vascularization index” model did not accurately predict the absence of a pathological tumor response to treatment (60.9% and 58.7%, respectively), while predicting a positive response to treatment was relatively better (70.9% and 75.4%, respectively).
Conclusions
Diffuse Optical Spectroscopy Imaging appeared to be a robust tool for early predicting breast cancer response to chemotherapy. It may help identifying patients who need additional molecular genetic study of the tumor in order to find the source of resistance to treatment, as well as to correct the treatment regimen.
Aligning Fabry-specific treatment access with contemporary evidence: Considerations for the Australian setting
Authors: Nicholls Kathleen , Denaro Charles , Tchan Michel , Ellaway Carolyn , Bratkovic Drago , Fookes Megan , Campbell Sheridan , Thomas Mark 
Published: August 2022

Abstract
Background: Fabry disease is a rare, progressive, X-linked, lysosomal storage disorder characterized by a wide spectrum of heterogeneously progressive clinical phenotypes. In the absence of a cure, disease-specific therapy aims to improve symptoms, stabilize current disease and delay progression. In Australia access to available treatments requires patients to meet pre-specified criteria, which were established more than 15 years ago. To address this, a panel comprising two members of the patient association Fabry Australia and its Medical Advisory Committee conducted a review of the literature to help inform contemporary disease-specific treatment access for patients with Fabry disease in Australia. The findings from the literature were applied to develop consideration statements and recommendations for revised classification and treatment initiation criteria.Results: Access criteria for Fabry-specific treatment in Australia are not aligned with current clinical evidence. The current treatment access paradigm in Australia imposes disease-severity related restrictions on all patients. This prevents timely treatment initiation and compromises clinical outcomes. Recommendations include the definition of patient classification groups to accommodate classical and non-classical disease and a revised decision framework to establish treatment initiation criteria with more sensitivity to the different disease trajectories within these patient groups. Conclusions: It is hoped that the proposed consideration statements and recommendations will raise awareness of a wider range of clinical scenarios that could be considered to prompt the initiation of Fabry-specific disease therapy in Australia. Adoption of these recommendations could support more equitable and timely access to treatment and better align Australian practice with contemporary evidence and international guidelines.
Investigating structural, thermal and optical properties of lead oxide nano-particles synthesized by sol-gel combustion method
Authors: Mirza Shahid , Chishtia Mayur Ali , Sangawar Vijaya , Parale Prashant 
Published: August 2022

AbstractMetal and metal oxide nanoparticles (NPs) have piqued the interest of material scientists in recent years due to their unique physicochemical properties which are dependent on their size, shape, and chemical surroundings. The sol gel combustion is one of the most straightforward and practical technique for getting tiny and similar size and shape of the powder out of all the current lead oxide nanoparticle synthesis techniques. In this study, PbO nanoparticles are prepared using the sol-gel method followed by an auto combustion process. This research is aimed at the particle size, shape, thermal analysis, and optical properties of nanostructured PbO. Several microscopic, spectroscopic, and thermogravimetric analysis are employed for the structural characterization of lead oxide nanoparticles. Tiny lead oxide particles with a diameter of 60 nanometres have been prepared. The prepares sample's optical band gap was discovered to be 2.44 electronvolt. Thermal investigations indicate the thermal behaviour and stability of synthesized lead oxide powder and are described in details.
The effect of lumbar spine manipulation on biomechanical factors and perceived transient pain during prolonged sitting: A laboratory-controlled cross-sectional study
Authors: De Carvalho Diana E , Callaghan Jack 
Published: August 2022

Abstract
Background: Spine manipulation has been shown to affect muscle activity, posture, and pain. To date, no studies have examined the effect of manipulation on biomechanical factors during sitting. Therefore, the purpose of this study was to investigate the immediate effect of lumbar spine manipulation on trunk muscle activation, spine posture and movements, and perceived ratings of transient pain in asymptomatic adults during prolonged office sitting.Methods: Twenty healthy adults were recruited for a single laboratory session that included a standardized office sitting/data entry protocol (120 minutes total, 3 blocks of 40 minutes). Data were collected between July and August 2012. The first block (baseline) was immediately followed by two experimental blocks. Prior to the start of each experimental block, participants were transferred to a therapy plinth and placed side lying (right side down), and a random presentation of either a control or high velocity low amplitude thrust directed at L4/L5 was delivered. Continuous measures of muscle activity, spine posture, and spine movements were recorded throughout the sitting trials. Perceived transient pain was measured by visual analogue scale at 7.5-minute intervals (including immediately before and after the randomized maneuvers).Results: There were no significant differences in spine or pelvic posture or perceived back pain following either the manipulation or control maneuvers. Significantly reduced muscle activity and increased shifts of the lumbar spine angle were identified in the block following manipulation compared to both baseline and post control blocks. Conclusions: Spine manipulation does not appear to have an immediate impact on spine or pelvic posture but does appear to reduce muscle activity and increase spine movement during sitting. Neither manipulation nor the control maneuver provided significant reductions in perceived transient pain. Future work should replicate this study with a larger population in a field study. It may be worthwhile to explore the implication of reduced muscle activation and increased spine movements during prolonged sitting for office workers that receive manipulations or mobilizations during their workday.
Alginate Fiber Grafted Polyetheramine Driven High Ion Conductive and Flame-Retardant Solid Polymer Electrolyte for Lithium Metal Batteries
Authors: Wang Yanru , Fang Timing , Wang Siyu , Wang Chao , Li Daohao , Xia Yanzhi 
Published: August 2022

AbstractTraditional poly(ethylene oxide) (PEO)-based solid polymer electrolytes (SPEs) often suffer from inherent poor flame retardancy and unsatisfied ionic conductivity at room temperature, which seriously affect the safety and energy storage performance of lithium metal batteries (LMBs). Inspired by the mechanism of Li+conductive in SPEs, an alginate fiber grafted polyetheramine (AF-PEA) membrane, capable of efficient Li+transport and excellent flame retardancy is dedicatedly designed and synthesized as the backbone of SPEs for solid-state LMBs. Based on the intrinsic flame retardancy of the alginate fiber, the PEO casted AF-PEA (PEO@AF-PEA) shows self-extinguishing ability, and its Li+transport ability is enhanced by grafting the ion conductive PEA chain segment. The LiFePO4/Li battery assembled using PEO@AF-PEA SPEs exhibits high safety and excellent cycling performance (exceeds 100 mAh g− 1after 1500 cycles at 2 C current density and 80°C with less than 0.016% capacity decay of peer cycle). By simulating the transport and distribution of Li+in the AF-PEA, the PEA with moderate chain lengths can uniformly fill the Li+transport space between the alginate backbone to promote the Li+adsorption and the utilization of Li+anchoring points in PEA side chains, increasing the Li+transport rate and migration capacity.
Evaluation of cotton establishment methods and zinc nutritionon productivity,zinc uptake, zinc use efficiency and fibre quality of Btcotton (Gossypium hirsutum L.) in Upper Gangetic Plain of India
Authors: Paul Tarun , Mondal Prithusayak , Basak Pradip , Rana D. S. 
Published: August 2022

Abstract

A field experiment was conducted during rainy seasons of 2013 and 2014 to evaluate the performance of cotton establishment methods and zinc nutrition on zinc use efficiency, productivity and quality of Bt cotton. The experiment was laid out in split plot design comprising two cotton establishment methods (direct sowing and transplanting) and five zinc-levels (control, 2.5, 5.0, 7.5 kg zinc ha-1and 0.5% zinc sulphate foliar spray at 60 and 90 days after planting) on cotton. The cotton variety (SP 7007, BG II) was taken under study. Statistically similar seed cotton, lint, seed, stalk and biological yield were recorded under methods of crop establishment. Zinc levels significantly influenced seed cotton, lint, seed, stalk, biological yields and ginning percentage in both the years and application of 5.0 and 7.5 kg Znha-1being at par recorded significantly higher seed cotton, lint, seed, stalk and biological yield over 2.5 kg Znha-1and control. Foliar application of 0.5 % zinc recorded seed cotton, lint, seed, stalk and biological yields on par with 2.5 kg Znha-1and significantly inferior to 5.0 and 7.5 kg Znha-1. On an average, application of 5.0 kg Znha-1recorded 9.2, 14.5, 8.3, 7.6 and 7.1 % increase in seed cotton, lint, seed, stalk and biological yields over control. Cotton establishment methods recorded non-significant in quality parameters, however, by and large improvement in all the quality parameters was observed with increasing level of zinc and highest value of ginning percentage, uniformity ratio, 2.5 % span length, 50 % span length and fibre elongation were recorded with 7.5 kg Znha-1, whereas in case of fibre strength, the highest value was recorded with 5.0 kg Znha-1. Transplanted plots recorded slightly higher zinc content than direct sown plots. The higher content of zinc in cotton seed and fibre was recorded due to effect of 5.0 and 7.5 kg Znha-1, both being at par across the seasons. Transplanted plots recorded slightly higher zinc uptake than direct sown plots. The higher uptake of zinc in cotton seed, fibre and total zinc uptake was recorded due to effect of 5.0 and 7.5 kg Zn ha-1in both the seasons, which was significantly superior to foliar application, 2.5 kg Znha-1and control. Effect of methods of crop establishment was non-significant on agronomic zinc use efficiency, zinc recovery efficiency, physiological efficiency and zinc harvest index. Increase in level of zinc and foliar spray decreased the agronomic zinc use efficiency.