bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative Uric Acid-to-albumin Ratio as a New Indicator for Predicting Long-term Prognosis in Patients With Acute Type a Aortic Dissection
Authors: Wang Xue , Deng Chao , Guo Fengwei , Zhong Liang , Li Ming , Xue Yanbo , Ma Qiong , Zheng Xiaopu , Gao Heng 
Published: January 2022

Abstract
Objectives— We aimed to analyze the ability of the preoperative uric acid-to-albumin ratio (UAR) to predict the postoperative long-term mortality of patients with type A AAD.Methods —A total of 289 patients with type A AAD who were enrolled in this study. First, all patients were divided into two groups: UAR < 9.875 and UAR ≥ 9.875. Furthermore, according to whether death occurred after one year after surgery, the patients were divided into the non-survival and survival groups. Univariable and multivariable COX regression analyses were conducted to identify the independent risk factors for long-term death in patients with type A AAD. Receiver operating characteristic (ROC) curves were constructed to assess the values of these indices in predicting postoperative mortality. Results— Age, hypertension, albumin content, UAR, and D-dimer content were found to be independent risk factors for mortality. The area under the ROC curve (AUC) of UAR was 0.618(HR 1.904, 95% CI, 1.097 to 3.305; P< 0.05), the combination of UAR, age and D-dimer was best for predicting deaths,the area under the AUC of them was 0.751 [HR 1.904,95% CI,0.681 to 0.821; P< 0.05)].Conclusions—In patients with type A AAD, UAR may considered as a new independent risk factor for long-term mortality. a combination of UAR, age, and D-dimer content was a best predictor of deaths.
Effect of early morning awakening in major depressive disorder
Authors: Xu Guohong , Yu Yang , Tang Chaohua , Liu Zishan , Li Xiaoling , Tan Yukang , Mai Xiancong , Li Runyi , Xu Caixia , Xie Guojun , Liang Jiaquan 
Published: January 2022

Abstract
Background Patients with major depressive disorder (MDD) usually manifest sleep disturbance. Early morning awakening is more closely related to MDD than other sleep disturbances. Aim The aim of this study was to assess the effect of early morning awakening in the treatment of patients with MDD. Methods Eligible patients were randomly assigned into two groups: early morning awakening and non-early morning awakening group. All patients were assessed using Hamilton Depression Scale (HAMD), Hamilton Anxiety Scale (HAMA), and Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) scores at baseline and the fourth week. Results Twenty-one men and 31 women (mean age 25.13 ±10.67 years) were enrolled. There is a significant main effect of early morning awakening in HAMD (P =0.04) and HAMA (P =0.01) at fourth week after escitalopram treatment. Discussion This trial suggests that early morning awakening may result in statistically and clinically significant delay in the recovery of the MDD but cognitive impairment.
Treatment results for patients with squamous-cell carcinoma of the anus, a single institution retrospective analysis
Authors: Neelis KJ , Kip Django , Speetjens Frank , Linden Yvette van der 
Published: January 2022

Abstract
Background and purpose

To gain insight into the treatment outcomes for anal cancer at a retrospective analysis was performed with a special emphasis on trends in outcome and toxicities over time and on treatment of elderly patients.
Materials and methods

Medical records of 98 consecutive patients with squamous cell carcinoma of the anus of all stages treated with curative intent between 01-01-2009 and 31-12-2018 were analyzed with follow up until 31-12-2020. Standard tumor and pathological lymph node dose were 59.4Gy or 60Gy, elective nodal regions were treated with 45Gy. Radiotherapy techniques in this period evolved from 3D conformal to IMRT and VMAT. In 23 patients electron beams were used.
Results

Median age was 63 years (range 41 – 88), majority female patients (60%). Twenty three patients were > 75 years old. The TNM stages were I, II, IIIA, and IIIB in 18%, 40%, 15% and 27%, fifty-eight % of patients had N0 status. Concurrent mitomycin C and 5-fluoruracil-based chemotherapy was given in 63 patients (64%). Five-year overall survival (OS), disease free survival (DFS), locoregional control (LRC) and colostomy free survival (CFS) were 71%, 80%, 82%, and 82% for the whole group. Results in patients > 75 years of age were not statistically different from those in younger patients. With the introduction of more conformal techniques DFS did not change and toxicities decreased.
Conclusion

Real word treatment outcomes per disease stage were in line with what is reported in literature. Older patients should also be offered treatment with curative intent.
HOXA11-AS1 promotes PD-L1 mediated immune escape and metastasis of hypopharyngeal carcinoma by facilitating the association of PTBP1 with FOSL1
Authors: Zhou Zheng , Liu Qian , Zhang Gehou , Mohammed Diab , Amadou Sani , Tan Guolin , Zhang Xiaowei 
Published: January 2022

Abstract
Background
The metastatic characteristics of hypopharyngeal squamous cell carcinoma (HSCC) led to many diagnostic and therapeutic challenges. The identification of functional long non-coding RNAs (lncRNAs) would contribute to investigate effective strategies for the diagnosis and treatment of HSCC.
Methods
Research was carried out in HSCC tissues and cells. RT-qPCR, western blot, immunohistochemistry and immunofluorescence assay were used to detect the related gene expression. Flow cytometry was used to measure the percentage of CD8+ and CD4+ T cells. CCK-8, colony formation, wound healing and transwell assays were performed to analyze the functional role of HOXA11-AS1. The targeted relationship of FOSL1/PD-L1 promoter was measured by ChIP and dual-luciferase reporter assays. RNA pull-down and RIP assays were used to measure the interaction between HOXA11-AS1, FOSL1 and PTBP1. Tumor xenograft study was used to analyze the function of HOXA11-AS1 in vivo.
Results
HOXA11-AS1, PD-L1 and FOSL1 were up-regulated in HSCC, and HOXA11-AS1 was positively correlated with PD-L1. HOXA11-AS1 knockdown upregulated CD8+T cells and the concentration of IFN-γ, while decreased the proliferation, migration and invasion of HSCC cells. FOSL1 bound to PD-L1 promoter and increased its expression. HOXA11-AS1 enhanced the stability of FOSL1 mRNA by binding to PTBP1. Overexpression of HOXA11-AS1 or PTBP1 increased the expression of FOSL1 and PD-L1. Knockdown of PTBP1 blocked the enhancement of HOXA11-AS1 overexpression on FOSL1 and PD-L1 levels. Knockdown of PD-L1 arrested the inhibiting function of HOXA11-AS1 overexpression on CD8+ T cells content, and the promoting effect on cell proliferation and metastasis. Knockdown of HOXA11-AS1 inhibited the immune escape and metastasis through regulating PD-L1 by down-regulating FOSL1 in vivo.
Conclusion
HOXA11-AS1 promoted PD-L1 expression through up-regulating FOSL1 levels by PTBP1, thereby facilitating the immune escape, proliferation and metastasis of HSCC cells.
Echinococcus Granulosus Extracellular Vesicles miRNA Cargo Regulates the Encystation of Protoscoleces via Notch Signaling Pathway in Vitro
Authors: Gao Jian , Zhou Xuan , Liu Ling , Lv Guodong , Hou Qiulian , Zhang Xiaofan , Shen Yujuan 
Published: January 2022

Abstract
Background: Since the protoscolece(PSCs) of Echinococcus granulosus has the characteristics of differentiation into hydatid in the intermediate host, it may be the main cause of secondary hydatid infection and dissemination. Extracellular vesicles (EVs) can transfer non-coding RNAs especially small RNAs (miRNAs) into parasite cells to regulate their gene expression. However, developmental pathway-related miRNAs such as the Notch signaling pathway loaded by EVs are not fully characterized. Therefore, the current study aimed to screen mRNA-miRNA interaction genes involved in the Notch signaling pathway during the encystation of E. g in vitro, and observe their expression changes in the parasite and extracellular vesicles(EVs).Methods: The miRNA and mRNA profiles of PSCs and microcysts (MCs) of E. g were analyzed using high-throughput sequencing respectively. Differential mRNAs between PSCs and MCs were overlapped with mRNAs which were predicted from differential miRNAs between PSCs and MCs. Functions of candidate differential mRNAs were predicted using Gene Ontology enrichment, Kyoto Encyclopedia of Genes, and Genomes pathway analysis. The miRNA-mRNA subnetwork related to the Notch signaling pathway was established. The expression of Notch pathway-related miRNAs and its target mRNAs in the worms and EVs at different encystation time points by Quantitative Reverse Transcription–Polymerase Chain Reaction(qRT-PCR).Results: 9439 mRNAs were predicted from 39 differential miRNAs between PSCs and MCs and overlapped with 1586 differential mRNAs in the transcriptome library, 1445 differential mRNAs were screened out and clustered into 94 metabolic pathways. 5 differential miRNAs corresponding to 4 target mRNAs were enriched in the Notch signaling pathway, especially containing a new unknown miRNA egl-m0694-3p. The 4 target mRNAs, EgrG_000892700, EgrG_001029400, EgrG_001081400 and EgrG_000465800 were respectively annotated as TACE, CSL, CtBP, and Delta in the Notch signaling pathway. EgrG_000892700, which was annotated as ADAM17 / TACE in the Notch signaling pathway, was down-regulated during encystation process of PSCs. Three miRNAs, egr-miR-4989-3p, egr-miR-9-3p and egr-miR-277a-3p might have negative regulatory effects on EgrG_000892700. The expressions of egr-miR-4989-3p and egr-miR-277a-3p in EVs of MCs were nearly 5 times that of PSCs.Conclusions: EVs may negatively regulate the expression of EgrG_000892700 in worms through continuous targeting of egr-miR-4989-3p and egr-miR-277a-3p, inhibit the transcription of the Notch signaling pathway, and may participate in the regulation of encystation of E. granulosus.
Sulfamethoxazole: Molecular Docking and Crystal Structure Prediction
Authors: Arputharaj David Stephen , Meenashi Rajasekaran , Pannipara Mehboobali , Al-Sehemi Abdullah Godran , rahman Mohamed Mustafa Abdul 
Published: January 2022

Abstract
The conformational stability of sulfonamide derivatives was examined in the active site of Dihydropteroate synthase enzyme. The best docked conformer was identified via molecular docking approach. Possible stable packing of best docked conformer was predicted and evaluated from the intermolecular interactions. Further, the interactions of stable conformer with the active site residue was characterized by charge density analysis based on Bader’s Quantum Theory of Atoms in Molecule.
dGLYAT modulates Gadd45-mediated JNK activation and cell invasion
Authors: Xue Lei , Xu Meng , Ren Pu , Tian Juhui , Xiao Lisha , Hu Ping , Chen Ping , Xie Xin , Li Wenzhe 
Published: January 2022

Abstract
Background: Cell invasion is a crucial step of tumor metastasis, finding new regulators of which offers potential drug targets for cancer therapy. Aberrant GLYAT expression is associated with human cancers, yet its role in cancer remains unknown. This study aims to understand the function and mechanism of Drosophila GLYAT in cell invasion.Results: We found that dGLYAT regulates Gadd45-mediated JNK pathway activation and cell invasion. Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. Secondary, mRNA-seq analysis identified Gadd45 as a potential transcriptional target of dGLYAT, as depletion of dGLYAT decreased Gadd45 mRNA level. Finally, Gadd45 knockdown suppressed scrib depletion-induced JNK pathway activation and cell invasion. Conclusions: These evidences reveal the role of dGLYAT and Gadd45 in cell polarity disruption-induced JNK-dependent cell invasion, and provid insight for the roles of their human homologs in cancers.
Student’s Academic Performance Prediction – A Review
Authors: Sudais Muhammad , Asad Danish 
Published: January 2022

Abstract
Providing quality education to students is the main objective of higher education institutions. The need of identifying students with weak performances has been a rising problem and most teachers have relied on calculating the average of exam grades. The main objective of our project is to predict and identify the students who might fail in semester examinations. This would prove helpful for teachers in providing additional assistance to such students. This review is conducted to find out how different researchers have approached this problem, what the outcomes of their study are and how it can help us in improving the performance of students. This review shows that machine learning, clustering proves useful in predictions, but there is a lot more work to be done using this technology.
Effects of institutionalizing limited obstetric ultrasound services on utilization of maternal and neonatal health services: a pre-post study
Authors: Abawollo Hailemariam Segni , Argaw Mesele Damte , Tsegaye Zergu Tafesse , Beshir Ismael Ali , Guteta Asfaw Adugna , Heyi Atrie Fekadu , Mengesha Birhan Tenaw , Mamo Tsega Teferi , Gebremedhin Zenawork Kassahun , Damte Heran Demise , Kibret Mengistu Asnake , Desta Binyam Fekadu 
Published: January 2022

Abstract
Background
A minimum of one ultrasound scan is recommended for all pregnant women before the 24th week of gestation. In Ethiopia, there is a shortage of skilled manpower to provide these services. Currently, trained mid-level providers are providing the services at the primary healthcare level.
Methods
A pre and post intervention observational study was conducted to understand maternal health service utilization rates before and after institutionalizing Vscan access limited obstetric ultrasound services between 2017 and 2020.
Results
The observed monthly increase on the mean rank of first ANC visits after the introduction of Vscan limited obstetric ultrasound services shows a statistically significant difference at KW-ANOVA H (3) =17.09, P=0.001. The mean rank of fourth ANC utilization increment shows statistically significant difference at KW- ANOVA H (3) =16.24, P=0.001. The observed mean rank in skilled birth attendance shows a statistically significant positive difference using KW-ANOVA H (3) =23.6, P<0.001. The mean rank of increased utilization in postnatal care shows a statistically significant difference using KW-ANOVA H (3) =17.79, P<0.001.
Conclusion
The introduction of limited obstetric ultrasound services by trained mid-level providers at primary healthcare levels was found to have improved the utilization of antenatal care, skilled birth attendance, and postnatal care services. It is recommended that limited obstetric ultrasound services be scaled up, and a further comparative study between facilities with and without ultrasound services be conducted to assess the effect of the introduction of the services on maternal and perinatal outcomes.
Barriers to Condom Use Among Heterosexuals in Africa; the Niger Delta Case Study
Authors: Ebeh Dandison Nat , Inungu Joseph N , Akinmoladun Tunde M , Minelli Mark J 
Published: January 2022

Abstract
Introduction

The search for an effective response and solution to the human immunodeficiency virus (HIV) / acquired immunodeficiency syndrome (AIDS) scourge continues to be of critical importance since the discovery of the first case in the United States in 1981. To date, in the absence of a known cure or vaccine, preventive and promotional measures remain a strategic measure to combating the menace.

Objectives

To identity barriers to condom use amongst heterosexuals in Africa, using the Niger Delta as a case study.

Methodology

This qualitative study was carried out using an online social media interactive medium. Ten experienced medical doctors from the Niger Delta subregion, recruited through a convenient snowballing technique, participated in the focus group discussion.

Results

Twelve elements were identified as barriers to condom use. They include poverty (worsened by environmental oil pollution), low and uneven distribution of knowledge and awareness, negative perception of condoms, impaired motivation, partner-related factors, lack of availability or access to a condom, religious factors, young age at first sexual debut affecting cognition to negotiate condom use, alcohol and substance use, intimate partner violence arising from gender inequality, cultural beliefs, social stigma, and accidental casual sex.

Conclusion

The findings of this study demonstrated the salient need for implementing a tailored health intervention program alongside the provision of public health measures. Awareness alone is not enough unless it translates into a behavioral change and control.
Ontogeny of the elemental composition and the biomechanics of radular teeth in the chiton Lepidochitona cinerea
Authors: Krings Wencke , BRÜTT JAN-OLE , GORB STANISLAV N. 
Published: January 2022

Abstract
Background

The radula, a chitinous membrane with embedded teeth, is one important molluscan autapomorphy. In some taxa (Polyplacophora and Patellogastropoda) one tooth type (the dominant lateral tooth) was studied intensively in the last decades with regard to its mechanical properties, chemical, and structural composition, and the relationship between these parameters. As the dominant lateral tooth is probably one of the best studied biological materials, it is surprising, that data on chemistry and mechanical properties of the other tooth types, present on a chiton radula, is lacking.
Results

We provide data on the elemental distribution and mechanical properties (hardness and elasticity, i.e. Young’s modulus) of all teeth from the Polyplacophora Lepidochitona cinerea (Linnaeus, 1767) [Chitonidae: Ischnochitonidae]. The ontogeny of elements, determined by energy-dispersive X-ray spectroscopy, and of the mechanical properties, detected by nanoindentation, was studied for every single tooth type. Additionally, we performed breaking stress experiments with teeth under dry and wet condition, highlighting the high influence of the water content on the mechanical behaviour of the radula. We thereby could determine the forces and stresses, teeth can resist, which was previously not studied in representatives of Polyplacophora. Overall, we were able to relate the mineral (iron, calcium) content, the mechanical parameters, breaking force, and stress for each tooth type. This led to a better understanding of the relationship between structure, material, and function in radular teeth. Further, we aimed at determining the role of calcium for the mechanical behaviour of the teeth: we decalcified radulae by ethylenediaminetetraacetic acid and afterwards performed elemental analyses, breaking stress experiments and determined the mechanical properties. Among other things, we detected that wet and decalcified radular teeth could resist highest forces, since teeth have a higher range of bending motion leading to a higher capability of teeth to gain mechanical support from the adjacent tooth row.
Conclusion

We present novel data on the chemistry, mechanical properties, and the mechanical behaviour of chiton teeth, which allows conclusions about tooth function. We could also relate the parameters mentioned, which contributes to our understanding on the origins of mechanical property gradients and the processes reducing structural failure in radular teeth.
Process Development for the Production and Purification of PEGylated
RhG-CSF Expressed in Escherichia coli
Authors: Mohammadi Zeinab , Alijanianzadeh Mahdi , Khalilzadeh Rassoul , Khodadadi Sirus 
Published: January 2022

Background and objective:
Recombinant human granulocyte-colony stimulating factor (rhG-CSF) and its PEGylated form (PEG-GCSF) are used in the cancer therapy. Thus the development of a more cost-effectively method for expressing rhG-CSF and the PEGylation optimization of rhG-CSF by reaction engineering and subsequent the purification strategy is necessary.

Methods:
RhG-CSF expression in Escherichia coli BL21 (DE3) was carried out by auto-induction batch fermentation and improved for maximizing rhG-CSF productivity. After that, purified rhG-CSF was PEGylated using methoxy polyethylene glycol propionaldehydes (mPEG20-ALD). The various conditions effect of extraction and purification of rhG-CSF and PEG-GCSF were assayed.

Results:
The assessment results revealed that auto-induction batch cultivation strategy had maximum productivity and rhG-CSF purity was more than 99%. The obtained Data of rhG-CSF PEGylation displayed that the optimized conditions of rhG-CSF PEGylation and purification enhanced hemogenisity PEG-GCSF and managed reaction toward optimal yield of PEG-GCSF (70%) and purity of 99.9%. Findings from FTIR, CD, and fluorescence spectroscopy and bioassay revealed that PEGylation was executed exactly in the rhG-CSF N-terminus, and products maintained their conformation properties.

Conclusion:
Overall, the developed approach expanded strategies for high yield rhG-CSF by simplified auto-induction batch fermentation system and rhG-CSF PEGylation, which are simple and time-saving, economical and high efficiency.
SLC17A2 Expression Correlates with Prognosis and Immune Infiltrates in
Hepatocellular Carcinoma
Authors: Jiang Zheng , Wang Zhijian , Chen Xuenuo 
Published: January 2022

Background:
Hepatocellular carcinoma (HCC) is one of the most common malignant
tumors with a dismal prognosis, according to updated statistics. The solute carrier family 17 member
2 (SLC17A2) has not been studied in liver cancer. Therefore, we evaluated the role of
SLC17A2 in HCC by bioinformatics analysis.

Objective:
The objective of the study was to explore the value of SLC17A2 in the prognosis and
diagnosis of hepatocellular carcinoma.

Method:
The expression level of SLC17A2 in HCC and the clinicopathological data were analyzed
based on The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases and
validated by immunohistochemical staining. In addition, the Kaplan–Meier plotter database and receiver
operating characteristic (ROC) curve analysis were used to explore the prognostic and diagnostic
significance. Some online databases were used to analyze the relationship between immune
cell infiltration and analyze the relationship between immune cell infiltration and SLC17A2 in
HCC.

Results:
Multivariate Cox regression analysis showed that SLC17A2 expression was low in HCC
(P < 0.05) and closely related to the clinical stage of HCC. In addition, SLC17A2 had a certain diagnostic
value in HCC according to ROC curve analysis. Further biological analyses showed that
SLC17A2 can regulate fatty acid metabolism, amino acid metabolism and cytochrome P450-
related metabolism. Notably, we found that SLC17A2 expression was closely correlated with the
infiltration of most immune cells in HCC.

Conclusion:
SLC17A2 expression is low in HCC and correlates with immune infiltration, so it
may serve as an independent prognostic factor for HCC.