bluuebunny/crossref_metadata_2025_split huggingface dataset:
The quantum oscillations in different probe configurations in the $$\hbox {BiSbTe}_{{3}}$$ topological insulator macroflake
Authors: Huang Shiu-Ming , Lin Chien , You Sheng-Yu , Yan You-Jhih , Yu Shih-Hsun , Chou Mitch 
Published: March 2022

AbstractWe demonstrate quantum oscillations in $$\hbox {BiSbTe}_{{3}}$$

BiSbTe
3

topological insulator macroflakes in different probe configurations. The oscillation period in the local configuration is twice compared to the non-local configuration. The Aharonov–Bohm-like (AB-like) oscillation dominates the transport property in the local configuration and the Altshuler–Aronov–Spivak-like (AAS-like) oscillation dominates the transport property in the non-local configuration. The AB-like oscillation period is 0.21 T and the related loop diameter is 156 nm which is consistent with the reported phase coherence length in topological insulators. The Shubnikov–de Haas oscillation frequency is the same but oscillation peaks reveal a $$\pi$$
π
phase shift in the local and non-local configuration. The Berry phase is $$\pi$$
π
in the local configuration and 0 in non-local configuration.
Analysis of the causes of inferiority feelings based on social media data with Word2Vec
Authors: Liu Yu , Xu Chen , Kuai Xi , Deng Hao , Wang Kaifeng , Luo Qinyao 
Published: March 2022

AbstractFeelings of inferiority are complex emotions that usually indicate perceived weakness and helplessness. A lack of timely and effective interventions may bring serious consequences to individuals with inferiority feelings. Due to privacy concerns, those people often hesitate to seek face-to-face help, but they usually spontaneously share their feelings on social media, which makes social media a good resource for ample inferiority-related data. We randomly selected a sample of posts indicating inferiority feelings to explore the causes of inferiority. Through language analysis and natural language processing, we constructed a Word2Vec model of inferiority based on social media data and applied it to the cause analysis of inferiority feelings. The main causes of inferiority feelings are personal experience, social interaction, love relationship, etc. People feeling inferior about their personal experiences usually are largely influenced by their ways of thinking and life attitudes. Social and emotional factors overlap somewhat in the development of inferiority. In love relationships, males are more prone to inferiority feeling than females. These findings will help relevant institutions and organizations better understand people with inferiority feelings and facilitate the development of targeted treatment for those with potential self-esteem problems.
Spatial–temporal patterns and risk factors for human leptospirosis in Thailand, 2012–2018
Authors: Chadsuthi Sudarat , Chalvet-Monfray Karine , Geawduanglek Suchada , Wongnak Phrutsamon , Cappelle Julien 
Published: March 2022

AbstractLeptospirosis is a globally important zoonotic disease. The disease is particularly important in tropical and subtropical countries. Infections in humans can be caused by exposure to infected animals or contaminated soil or water, which are suitable for Leptospira. To explore the cluster area, the Global Moran’s I index was calculated for incidences per 100,000 population at the province level during 2012–2018, using the monthly and annual data. The high-risk and low-risk provinces were identified using the local indicators of spatial association (LISA). The risk factors for leptospirosis were evaluated using a generalized linear mixed model (GLMM) with zero-inflation. We also added spatial and temporal correlation terms to take into account the spatial and temporal structures. The Global Moran’s I index showed significant positive values. It did not demonstrate a random distribution throughout the period of study. The high-risk provinces were almost all in the lower north-east and south parts of Thailand. For yearly reported cases, the significant risk factors from the final best-fitted model were population density, elevation, and primary rice crop arable areas. Interestingly, our study showed that leptospirosis cases were associated with large areas of rice production but were less prevalent in areas of high rice productivity. For monthly reported cases, the model using temperature range was found to be a better fit than using percentage of flooded area. The significant risk factors from the model using temperature range were temporal correlation, average soil moisture, normalized difference vegetation index, and temperature range. Temperature range, which has strongly negative correlation to percentage of flooded area was a significant risk factor for monthly data. Flood exposure controls should be used to reduce the risk of leptospirosis infection. These results could be used to develop a leptospirosis warning system to support public health organizations in Thailand.
Microwell bag culture for large-scale production of homogeneous islet-like clusters
Authors: Suenaga Ryo , Konagaya Shuhei , Yamaura Junji , Ito Ryo , Tanaka Satoshi , Ishizaki Yoichi , Toyoda Taro 
Published: March 2022

AbstractPluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 105–106 islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to be overcome for its clinical use. In this study, we aimed to develop a novel bag culture method for the production of uniform cell clusters on a large scale (105–106 clusters). We prepared small-scale culture bags (< 105 clusters) with microwells at the bottom and optimized the conditions for producing uniform-sized clusters in the bag using undifferentiated induced pluripotent stem cells (iPSCs). Subsequently, we verified the suitability of the bag culture method using iPSC-derived pancreatic islet cells (iPICs) and successfully demonstrate the production of 6.5 × 105 uniform iPIC clusters using a large-scale bag. In addition, we simplified the pre- and post-process of the culture—a degassing process before cell seeding and a cluster harvesting process. In conclusion, compared with conventional methods, the cluster production method using bags exhibits improved scalability, sterility, and operability for both clinical and research use.
Transcriptomic analysis of methyl jasmonate treatment reveals gene networks involved in drought tolerance in pearl millet
Authors: Ndiaye Adama , Diallo Amadou Oury , Fall Ndèye Coura , Diouf Rose Diambogne , Diouf Diaga , Kane Ndjido Ardo 
Published: March 2022

AbstractWater deficit stress at the early stage of development is one of the main factors limiting pearl millet production. One practice to counteract this limitation would be to resort to the application of hormones to stimulate plant growth and development at critical stages. Exogenous methyl jasmonate (MeJA) can improve drought tolerance by modulating signaling, metabolism, and photosynthesis pathways, therefore, we assumed that can occur in pearl millet during the early stage of development. To decipher the molecular mechanisms controlling these pathways, RNAseq was conducted in two pearl millet genotypes, drought-sensitive SosatC88 and drought-tolerant Souna3, in response to 200 μM of MeJA. Pairwise comparison between the MeJA-treated and non-treated plants revealed 3270 differentially expressed genes (DEGs) among 20,783 transcripts in SosatC88 and 127 DEGs out of 20,496 transcripts in Souna3. Gene ontology (GO) classification assigned most regulated DEGs in SosatC88 to heme binding, oxidation–reduction process, response to oxidative stress and membrane, and in Souna3 to terpene synthase activity, lyase activity, magnesium ion binding, and thylakoid. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis reveals that DEGs in SosatC88 are related to the oxidation–reduction process, the biosynthesis of other secondary metabolites, the signal transduction, and the metabolism of terpenoids, while in Souna3, DEGs are related to the metabolism of terpenoids and the energy metabolism. Two genes encoding a diterpenoid biosynthesis-related (Pgl_GLEAN_10009413) and a Glutathione S transferase T3 (Pgl_GLEAN_10034098) were contra-regulated between SosatC88 and Souna3. Additionally, five random genes differentially expressed by RNAseq were validated using qPCR, therefore, they are potential targets for the development of novel strategies breeding schemes for plant growth under water deficit stress. These insights into the molecular mechanisms of pearl millet genotype tolerance at the early stage of development contribute to the understanding of the role of hormones in adaptation to drought-prone environments.
Identification of hub genes for adult patients with sepsis via RNA sequencing
Authors: Zhang Qian , Hu Yingchun , Wei Peiyao , Shi Liu , Shi Lei , Li Jianzhou , Zhao Yalei , Chen Yunru , Zhang Xi , Ye Feng , Liu Xiaojing , Lin Shumei 
Published: March 2022

AbstractTo screen out potential prognostic hub genes for adult patients with sepsis via RNA sequencing and construction of a microRNA–mRNA–PPI network and investigate the localization of these hub genes in peripheral blood monocytes. The peripheral blood of 33 subjects was subjected to microRNA and mRNA sequencing using high-throughput sequencing, and differentially expressed genes (DEGs) and differentially expressed microRNAs (DEMs) were identified by bioinformatics. Single-cell transcriptome sequencing (10 × Genomics) was further conducted. Among the samples from 23 adult septic patients and 10 healthy individuals, 20,391 genes and 1633 microRNAs were detected by RNA sequencing. In total, 1114 preliminary DEGs and 76 DEMs were obtained using DESeq2, and 454 DEGs were ultimately distinguished. A microRNA–mRNA–PPI network was constructed based on the DEGs and the top 20 DEMs, which included 10 upregulated and 10 downregulated microRNAs. Furthermore, the hub genes TLR5, FCGR1A, ELANE, GNLY, IL2RB and TGFBR3, which may be associated with the prognosis of sepsis, and their negatively correlated microRNAs, were analysed. The genes TLR5, FCGR1A and ELANE were mainly expressed in macrophages, and the genes GNLY, IL2RB and TGFBR3 were expressed specifically in T cells and natural killer cells. Parallel analysis of mRNAs and microRNAs in patients with sepsis was demonstrated to be feasible using RNA-seq. Potential hub genes and microRNAs that may be related to sepsis prognosis were identified, providing new prospects for sepsis treatment. However, further experiments are needed.
Mapping the purple menace: spatiotemporal distribution of purple loosestrife (Lythrum salicaria) along roadsides in northern New York State
Authors: Rogers Jessica , Humagain Kamal , Pearson Adam 
Published: March 2022

AbstractPurple loosestrife (Lythrum salicaria L.) is an invasive, herbaceous plant, frequently found in wetlands, creating monoculture stands, resulting in intensive management strategies in central New York, Ontario, and Quebec. The goal of this study was to identify the extent of infestations and to investigate factors that promote the spread of purple loosestrife. We attempted to answer several questions regarding level of infestation, connection to mowing, and influence of culverts. During flowering season in July and August, 2017–2019, we mapped infestations along 150 km (93 miles) of state highway between the Adirondack Park and the St. Lawrence River using the ESRI Collector app. The results of our preliminary analysis revealed significant increase in the number of plants (P < 0.001). In addition, a linear correlation analysis demonstrated a higher loosestrife density with an increase in plant species richness and a decrease in the distance to the closest infestation and wetland (P < 0.001 each). We found no statistical evidence that mowing promotes the spread of loosestrife. As expected, there were more individual infestations in highway ditches, but larger and denser infestations in wetlands (P = 0.003 in 2019). Culverts enable purple loosestrife to spread underneath highways and should be managed to prevent spread.
House dust mite exposure enhances immune responses to ovalbumin-induced intestinal allergy
Authors: Lin Jianli , Chen Desheng , Guan Lvxin , Chang Kexin , Li Dan , Sun Baoqing , Yang Pingchang , Liu Zhigang 
Published: March 2022

AbstractHouse dust mites (HDM) are one of the important factors of airway allergic diseases, HDM allergens can be detected in the human gut mucosa, which induces local inflammation and increases intestinal epithelial permeability. This study tests a hypothesis that HDM contribute to the development of OVA (ovalbumin)-induced intestinal allergy. The serum levels of IgE against HDM in patients with food allergy were detected with UniCAP100 (Pharmacia, Uppsala, Sweden); a mouse model of food allergy was developed with OVA and HDM as the specific antigens. Compared to healthy controls, patients with food allergy have higher levels of serum HDM-specific IgE. Compared to food allergy alone groups, the levels of HDM-specific IgE in patients with food allergy and asthma or allergic rhinitis were significantly higher. In mouse models, we found that HDM/OVA induced allergy-like symptoms, lower body temperature, and lower body weight. The levels of IgE, IgG1, mMCP-1 (mouse mast cell protease-1), IL-4 and IL-5 in the HDM and HDM + CT (cholera toxin) groups were higher than the control groups, and the levels of IgE, IgG1, IL-4 and IL-5 in the HDM, OVA and HDM + OVA groups were higher than the control groups. The pathological changes of intestinal tissues in the HDM and HDM + CT/the HDM, OVA and HDM + OVA groups were more severe, more eosinophil infiltration than the control groups. Moreover, exposure to HDM induced intestinal barrier dysfunction, and facilitated the development of intestinal allergy in mice. In conclusion, HDM exposure enhances immune responses to OVA-induced food allergy.
High resolution 2D beam steerer made from cascaded 1D liquid crystal phase gratings
Authors: de Blas Mario García , García Javier Pereiro , Andreu Sergio Vera , Arregui Xabier Quintana , Caño-García Manuel , Geday Morten Andreas 
Published: March 2022

AbstractOptical beam steering (BS) has multiple applications in fields like target seeking and tracking, optical tweezers, billboard displays and many others. In this work, a two-dimensional beam deflector based on blaze gratings is presented. Phase-only 1D blaze gratings have been prepared using maskless Direct Laser Writing (DLW) resulting in high-resolution structures in indium-tin oxide (ITO) coated glass wafers. The device is composed of two identical 1D liquid crystal (LC) cells cascaded orthogonally back-to-back, with a resultant active area of 1.1 × 1.1 mm2. The 1D cells have been prepared with 144 pixels each with a 7.5 µm pitch. The total 288 pixels are driven by a custom made 12-bit Pulse Width Modulation (PWM) electronic driver, allowing for an arbitrarily high resolution. The system performance is documented, and the efficiency of the system has been tested. A maximum diagonal steering angle of ± 3.42° was achieved.
Atmospheric rivers fueling the intensification of fog and haze over Indo-Gangetic Plains
Authors: Verma Shivali , Ramana Muvva V. , Kumar Raj 
Published: March 2022

AbstractIndo-Gangetic Plains (IGP) experiences persistent and widespread rise of fog and haze during the winter season. This has been attributed to the rise in pollution levels and water vapor, but the reason for enhancement in latter is not clear yet. We detect moisture incursion from Arabian Sea, a phenomenon called atmospheric rivers (AR), land-falling intermittently along 12–25° N corridor of the west-coast of India during winter; using satellite and reanalysis data. The total vertically integrated horizontal water vapor transport in AR-landfalls ranging from 0.7 × 108 to 2.2 × 108 kg/s; nearly five-orders of magnitude larger than the average discharge of liquid water from Indus River into Arabian Sea. These AR events are playing prominent role in enhancing water vapor over IGP region by 19 ± 5%; in turn fueling the intensification of fog and haze through aerosol-water vapor interaction. We found that AR events enhanced aerosol optical depths over IGP by about 29 ± 13%. The progression of moist-laden winds in ARs onto Himalayan Mountains contributes to the precipitation that explains the observed rise in the extreme flow of western Himalayan Rivers in winter. We conclude that these ARs likely contribute to the decline of snow albedo as pollution-mixed-ARs encounter Hindukush-Karakoram-Himalayan mountain region.
Prevalence of and factors associated with atypical presentation in bacteremic urinary tract infection
Authors: Komagamine Junpei , Yabuki Taku , Noritomi Daichi , Okabe Taro 
Published: March 2022

AbstractA delay in the diagnosis of urinary tract infection (UTI) is not uncommon. Atypical presentation is often cited as one of the causes of diagnostic delays. However, few studies have investigated the prevalence of atypical presentation and determined factors associated with atypical presentation at initial contact among patients with UTI. Therefore, a retrospective and prospective cohort study using chart review was conducted in two acute care hospitals. We included 285 consecutive patients hospitalized for bacteremic UTI. The primary outcome was atypical presentation, defined as the absence of any urinary tract symptom or sign at initial contact. Of all patients, the median age was 82 years, 186 (65.3%) were women, and 53 (18.6%) had dementia. Urinary tract symptoms and signs were absent at initial contact in 144 patients (50.5%; 95% CI 44.7–56.4%). The multivariable analysis revealed that older age, male sex, dementia, and early visit from symptom onset were significantly associated with an increased risk of atypical presentation. Patients with atypical presentation were less likely to receive a correct diagnosis at initial contact than patients with urinary tract symptoms and signs (OR 0.30; 95% CI 0.17–0.51). Atypical presentation in patients with bacteremic UTI is common and negatively affects the correct diagnosis of UTI.
Foliar nutrient supplementation with micronutrient-embedded fertilizer increases biofortification, soil biological activity and productivity of eggplant
Authors: Bana Ram Swaroop , Jat Gograj Singh , Grover Minakshi , Bamboriya Shanti D. , Singh Deepak , Bansal Ruchi , Choudhary Anil K. , Kumar Vipin , Laing Alison M. , Godara Samarth , Bana Ramesh Chand , Kumar Hement , Kuri Bhola Ram , Yadav Achchhelal , Singh Teekam 
Published: March 2022

AbstractMicronutrient malnutrition or hidden hunger remains a major global challenge for human health and wellness. The problem results from soil micro- and macro-nutrient deficiencies combined with imbalanced fertilizer use. Micronutrient-embedded NPK (MNENPK) complex fertilizers have been developed to overcome the macro- and micro-element deficiencies to enhance the yield and nutritive value of key crop products. We investigated the effect of foliar applications of an MNENPK fertilizer containing N, P, K, Fe, Zn and B in combination with traditional basal NPK fertilizers in terms of eggplant yield, fruit nutritive quality and on soil biological properties. Applying a multi-element foliar fertilizer improved the nutritional quality of eggplant fruit, with a significant increases in the concentration of Fe (+ 26%), Zn (+ 34%), K (+ 6%), Cu (+ 24%), and Mn (+ 27%), all of which are essential for human health. Increasing supply of essential micronutrients during the plant reproductive stages increased fruit yield, as a result of improved yield parameters. The positive effect of foliar fertilizing with MNENPK on soil biological parameters (soil microbial biomass carbon, dehydrogenase, alkaline phosphatase) also demonstrated its capacity to enhance soil fertility. This study suggests that foliar fertilizing with a multi-nutrient product such as MNENPK at eggplant flowering and fruiting stages, combined with the recommended-doses of NPK fertilizers is the optimal strategy to improve the nutritional quality of eggplant fruits and increase crop yields, both of which will contribute to reduce micronutrient malnutrition and hunger globally.
Improving the accuracy and robustness of carotid-femoral pulse wave velocity measurement using a simplified tube-load model
Authors: Xu Lisheng , Zhou Shuran , Wang Lu , Yao Yang , Hao Liling , Qi Lin , Yao Yudong , Han Hongguang , Mukkamala Ramakrishna , Greenwald Stephen E. 
Published: March 2022

AbstractArterial stiffness, as measured by pulse wave velocity, for the early non-invasive screening of cardiovascular disease is becoming ever more widely used and is an independent prognostic indicator for a variety of pathologies including arteriosclerosis. Carotid-femoral pulse wave velocity (cfPWV) is regarded as the gold standard for aortic stiffness. Existing algorithms for cfPWV estimation have been shown to have good repeatability and accuracy, however, further assessment is needed, especially when signal quality is compromised. We propose a method for calculating cfPWV based on a simplified tube-load model, which allows for the propagation and reflection of the pulse wave. In-vivo cfPWV measurements from 57 subjects and numerical cfPWV data based on a one-dimensional model were used to assess the method and its performance was compared to three other existing approaches (waveform matching, intersecting tangent, and cross-correlation). The cfPWV calculated using the simplified tube-load model had better repeatability than the other methods (Intra-group Correlation Coefficient, ICC = 0.985). The model was also more accurate than other methods (deviation, 0.13 ms−1) and was more robust when dealing with noisy signals. We conclude that the determination of cfPWV based on the proposed model can accurately and robustly evaluate arterial stiffness.
Distribution and possible function of galanin about headache and immune system in the rat dura mater
Authors: Shimazaki Kenichiro , Yajima Takehiro , Ichikawa Hiroyuki , Sato Tadasu 
Published: March 2022

AbstractGalanin (GAL) is a nociceptive transmitter or modulator in the trigeminal sensory system. In this study, GAL expression was investigated in the rat dura mater to demonstrate its possible function in headache using immunohistochemical techniques. The cerebral falx and cerebellar dura mater received abundant blood and nerve supply, and were significantly thicker compared to other portions in the cerebral dura mater. GAL-immunoreactivity was expressed by cell and nerve fiber profiles. Presumed macrophages and dendritic cells contained GAL-immunoreactivity, and co-expressed with CD11b-immunoreactivity. Many isolated and perivascular nerve fibers also showed GAL-immunoreactivity. In addition, GAL-immunoreactive nerve fibers were present in the vicinity of macrophages and dendritic cells with either GAL- or ED1-immunoreactivity. GAL-immunoreactive cells and nerve fibers were common in the cerebral falx and cerebellar dura mater and infrequent in other portions. And, GAL-immunoreactive nerve fibers usually co-expressed calcitonin gene-related peptide (CGRP)-immunoreactivity. In the trigeminal ganglion, a substantial proportion of sensory neurons innervating the dura mater contained GAL-immunoreactivity (mean ± SD, 3.4 ± 2.2%), and co-expressed CGRP-immunoreactivity (2.7 ± 2.1%). The present study may suggest that GAL is associated with nociceptive transduction or modulation in the dura mater. GAL also possibly plays a role in the immune mechanism of the dura mater.