bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of COVID-19 Pandemic on Mental Health: A Scoping Review
Authors: Josiah Blessing O. , Ncube France 
Published: December 2022

AbstractBackgroundThis scoping review assessed the COVID-19 impacts on mental health and associated risk factors.MethodsA systematic literature search for relevant articles published in the period March 2020 to July 2022, was conducted in the APA PsychInfo, JBI Evidence Synthesis, Epistemonikos, PubMed, and Cochrane databases.ResultsA total of 72 studies met the inclusion criteria. Results showed that the commonly used mental health assessment tools were the Patient Health Questionnaire (41.7%), Generalized Anxiety Disorder Scale (36%), 21-item Depression, Anxiety, and Stress (13.9%), Impact of Event Scale (12.5%), Pittsburgh Sleep Quality Index (9.7%), Symptom Checklist and the General Health Questionnaire (6.9% each). The prevalence rate of depression ranged from 5-76.5%, 5.6-80.5% for anxiety, 9.1-65% for Post-Traumatic Stress Disorder, 8.3-61.7% for sleep disorders, 4.9-70.1% for stress, 7-71.5% for psychological distress, and 21.4-69.3% for general mental health conditions. The major risks included female gender, healthcare-related/frontline jobs, isolation/quarantine, poverty, lower education, COVID-19 risk, age, commodities, mental illness history, negative psychology, and higher social media exposure. The incidence of mental disorders increased along with the increasing cases of COVID-19 and the corresponding government restrictions.ConclusionStandard assessment tools were used for mental health assessment by the reviewed studies which were conducted during COVID-19. Mental health disorders like depression, anxiety, and stress increased during the COVID-19 pandemic and lockdowns. Various factors impacted the prevalence of mental health disorders. Policymakers need to provide social protective measures to improve coping capacities during critical health events to avoid negative impacts on the population. Further studies should investigate the effectiveness of interventions for reducing the prevalence and risk factors for mental health conditions during a public health challenge.Background
L-SCRaMbLE creates large-scale genome rearrangements in synthetic Sc2.0 chromosomes
Authors: Lindeboom Timon Alexander , Sánchez Olmos María del Carmen , Schulz Karina , Brinkmann Cedric Kilian , Ramírez Rojas Adán Andrés , Hochrein Lena , Schindler Daniel 
Published: December 2022

AbstractOptimization of the metabolic flux through heterologous pathways to improve bioproduction or utilization of alternative substrates requires both fine-tuning of non-native gene expression levels and improvement of the host genome. The SCRaMbLE system incorporated into synthetic Sc2.0 yeast strains enables a rapid approach to rearrange the genome ofSaccharomyces cerevisiaein order to create optimized chassis. Here, we show that the light-inducible Cre recombinase L-SCRaMbLE can efficiently generate diverse recombination events when applied to Sc2.0 strains containing a linear or circular synthetic chromosome III. We present an efficient and straightforward workflow for the identification of complex rearranged synthetic chromosomes from SCRaMbLEd isolates without selection pressure. The screening method is based on novel genotyping primers, theloxPsymtags, which indicate not only deletions but also inversions and translocations. Long-read Nanopore sequencing is used to decode the selected genotypes and shows in conjunction with flow cytometry that large-scale karyotype alterations can be a consequence of SCRaMbLE.
Conservation of antiviral systems across domains of life reveals novel immune mechanisms in humans
Authors: Cury Jean , Mordret Ernest , Trejo Veronica Hernandez , Tesson Florian , Ofir Gal , Poirier Enzo Z. , Bernheim Aude 
Published: December 2022

AbstractViral infection is a common threat to prokaryotic and eukaryotic life, which has resulted in the evolution of a myriad of antiviral systems. Some of these eukaryotic systems are thought to have evolved from prokaryotic antiphage proteins, with which they may display sequence and structural homology. Here, we show that homologs of recently discovered antiphage systems are widespread in eukaryotes. We demonstrate that such homologs can retain a function in immunity by unveiling that eukaryotic proteins of the anti-transposon piRNA pathway display domain homology with the antiphage system Mokosh. We further utilise this conservation to discover novel human antiviral genes related to the Eleos and Lamassu prokaryotic systems. We propose that comparative immunology across domains of life can be leveraged to discover immune genes in eukaryotes.
Coexisting morpho-biotypes unveil the regulatory bases of phenotypic plasticity in pancreatic ductal adenocarcinoma
Authors: Di Chiaro Pierluigi , Nacci Lucia , Brandini Stefania , Polletti Sara , Donati Benedetta , Gualdrini Francesco , Frige Gianmaria , Mazzarella Luca , Ciarrocchi Alessia , Zerbi Alessandro , Spaggiari Paola , Barozzi Iros , Diaferia Giuseppe R. , Natoli Gioacchino 
Published: December 2022

AbstractIntratumor morphological heterogeneity predicts clinical outcomes of pancreatic ductal adenocarcinoma (PDAC). However, it is only partially understood at the molecular level and devoid of clinical actionability. In this study we set out to determine the gene regulatory networks and expression programs underpinning intra-tumor morphological variation in PDAC. To this aim, we identified and deconvoluted at single cell level the molecular profiles characteristic of morphologically distinguishable clusters of PDAC cells that coexisted in individual tumors. We identified three major morpho-biotypes that co-occurred in various proportions in most PDACs: a glandular biotype with classical epithelial ductal features; a biotype with abortive ductal structures and expressing a partial epithelial-to-mesenchymal transition program; and a poorly differentiated biotype showing partial neuronal lineage priming and absence of both ductal features and basement membrane. The identification of PDAC morpho-biotypes may help improve patient stratification and therapeutic schemes taking into account the spectrum of actionable targets expressed by coexisting tumor components.
FK506-binding protein 2 participates in proinsulin folding
Authors: Hoefner Carolin , Bryde Tenna Holgersen , Pihl Celina , Tiedemann Sylvia Naiga , Bresson Sophie Emilie , Hotiana Hajira Ahmed , Khilji Muhammad Saad , Dos Santos Theodore , Puglia Michele , Pisano Paola , Majewska Mariola , Durzynska Julia , Klindt Kristian , Klusek Justyna , Perone Marcelo J. , Bucki Robert , Hägglund Per Mårten , Gourdon Pontus , Gotfryd Kamil , Urbaniak Edyta , Borowiak Malgorzata , Wierer Michael , MacDonald Patrick Edward , Mandrup-Poulsen Thomas , Marzec Michal Tomasz 
Published: December 2022

AbstractApart from chaperoning, disulphide bond formation and downstream processing, the molecular sequence of proinsulin folding is not completely understood. Proinsulin requires proline isomerization for correct folding. Since FK506-binding protein 2 (FKBP2) is an ER-resident proline isomerase, we hypothesized that FKBP2 contributes to proinsulin folding. We found that FKBP2 co-immunoprecipitated with proinsulin and its chaperone GRP94, and that inhibition of FKBP2 expression increased proinsulin turnover with reduced intracellular proinsulin and insulin levels. This phenotype was accompanied by an increased proinsulin secretion and the formation of proinsulin high molecular weight complexes, a sign of proinsulin misfolding. FKBP2 knockout in pancreatic β-cells increased apoptosis without detectable upregulation of ER-stress response genes. Interestingly, FKBP2 mRNA was overexpressed in β-cells from pancreatic islets of T2D patients. Based on molecular modelling and an in vitro enzymatic assay, we suggest that proline at position 28 of the proinsulin B chain (P28) is the substrate of FKBP2’s isomerization activity. We propose that this isomerization step catalyzed by FKBP2 is an essential sequence required for correct proinsulin folding.
Surface SV2A-Syt1 nanoclusters act as a sequestration hub that limits dynamin-1 recruitment and targeting to recycling synaptic vesicles
Authors: Small Christopher , Harper Callista , Jiang Anmin , Kontaxi Christiana , Yak Nyakuoy , Malapaka Anusha , Davenport Elizabeth C. , Wallis Tristan P. , Gormal Rachel S. , Joensuu Merja , Martínez-Mármol Ramon , Cousin Michael A. , Meunier Frédéric A. 
Published: December 2022

AbstractFollowing exocytosis, the recapture of plasma membrane-stranded vesicular proteins into recycling synaptic vesicles (SVs) is essential for sustaining neurotransmission. Surface clustering of vesicular proteins has been postulated as a ‘pre-assembly’ mechanism for endocytosis – ensuring high-fidelity retrieval. Here, we used single-molecule imaging to examine the nanoclustering of synaptotagmin-1 (Syt1) and synaptic vesicle protein 2A (SV2A) in hippocampal neurons. Syt1 forms surface nanoclusters through interaction of its C2B domain with SV2A, that are sensitive to mutations in this domain (Syt1K326A/K328A) and knocking down SV2A. SV2A co-cluster with Syt1 and blocking SV2A’s cognate interaction with Syt1 (SV2AT84A) also decreased SV2A clustering. Surprisingly, impairing SV2A-Syt1 nanoclustering enhanced plasma membrane recruitment of key endocytic protein dynamin-1, leading to accelerated Syt1 endocytosis, altered intracellular sorting and decreased trafficking of Syt1 to Rab5-positive endocytic compartments. SV2A-Syt1 surface nanoclusters therefore negatively regulate the rate of their own re-entry into recycling SVs by controlling the recruitment of the endocytic machinery.
Photosynthetic capacity in seagrass seeds and early-stage seedlings ofZostera marinaL
Authors: Brodersen Kasper Elgetti , Kühl Michael 
Published: December 2022

SummaryIn many terrestrial seeds, photosynthetic activity supplies O2to the developing plant embryo to sustain aerobic metabolism and enhance biosynthetic activity. However, whether seagrass seeds possess similar photosynthetic capacity to alleviate intra-seed hypoxic stress conditions is unknown.We used a novel combination of microscale variable chlorophyll fluorescence imaging, a custom-made O2optode microrespirometry system, and planar optode O2imaging, to determine the O2microenvironment and photosynthetic activity in developing seeds and seedlings of seagrass (Z. marinaL.).Developing, sheath-covered seeds exhibited high O2concentrations in the photosynthetic active seed sheath and low O2concentrations in the center of the seed at the position of the embryo. In light, photosynthesis in the seed sheath increased O2availability in central parts of the seed enabling enhanced respiratory energy generation for biosynthetic activity. Early-stage seedlings also displayed photosynthetic capacity in hypocotyl and cotyledonary tissues, which may be beneficial for seedling establishment.Sheath O2production is important for alleviating intra-seed hypoxic stress and can increase endosperm storage activity improving the conditions for successful seed maturation and germination.
R-loops and Topoisomerase 1 facilitate formation of transcriptional DSBs at gene bodies of hypertranscribed cancer genes
Authors: Hidmi Osama , Oster Sara , Monin Jonathan , Aqeilan Rami I. 
Published: December 2022

AbstractDNA double-stranded breaks (DSBs) pose a significant threat to genomic integrity, and their generation during essential cellular processes like transcription remains poorly understood. In this study, we employed advanced techniques to map DSBs, R-loops, and Topoisomerase 1 cleavage complex (TOP1cc) and re-analyzed ChIP-seq and DRIP-seq data to comprehensively investigate the interplay between transcription, DSBs, Topoisomerase 1 (TOP1), and R-loops. Our findings revealed the presence of DSBs at highly expressed genes enriched with TOP1 and R-loops, indicating their crucial involvement in transcription-associated genomic instability. Depletion of R-loops and TOP1 specifically reduced DSBs at highly expressed genes, uncovering their pivotal roles in transcriptional DSB formation. By elucidating the intricate interplay between TOP1cc trapping, R-loops, and DSBs, our study provides novel insights into the mechanisms underlying transcription-associated genomic instability. Moreover, we establish a link between transcriptional DSBs and early molecular changes driving cancer development. Notably, our study highlights the distinct etiology and molecular characteristics of driver mutations compared to passenger mutations, shedding light on the potential for targeted therapeutic strategies. Overall, these findings deepen our understanding of the regulatory mechanisms governing DSBs in hypertranscribed genes associated with carcinogenesis, opening avenues for future research and therapeutic interventions.
Examining critical project management skills for successful delivery of major maintenance projects: insights from the United Kingdom energy sector
Authors: Pieterse Bryan , Agyekum Kofi , Manu Patrick , Mohandes Saeed Reza , Cheung Clara , Yunusa-Kaltungo Akilu 
Published: December 2022

PurposeMajor maintenance projects are often regarded as maintenance activities regardless of the projects' complexity and scale. Consequently, very scarce research attention has hitherto been paid to the critical skills required when undertaking these projects. More specifically, the body of relevant knowledge is deprived of a study focusing on maintenance projects within the energy sector. In view of this shortcoming, this research aims to examine the critical project management (PM) skills required to deliver major maintenance projects within the energy sector.Design/methodology/approachBased on a quantitative research strategy, this study addressed the knowledge gap through a cross-sectional survey of professionals involved in the delivery of major maintenance projects in the United Kingdom's (UK) energy sector. Data obtained were analyzed via descriptive (e.g. frequencies, mean and standard deviation [SD]) and inferential statistical analyses (One sample t-test and exploratory factor analysis (EFA)).FindingsOut of the 45 PM skills identified in the literature and examined by the respondents, the results obtained from the One sample t-test (based on p (1-tailed) = 0.05) showed that 37 were considered to be at least “important,” accounting for 80.4% of all the skills identified. EFA revealed a clustering of the PM skills items into seven components: “skills related to work scheduling and coordination”; “communication, risk, safety and stakeholder management skills”; “quality assurance skills”; “people management skills”; “skills related to forecasting scope and duration of outage”; “implementation of processes and time management skills” and “technical/engineering skills and experience pertaining to the outage and local site knowledge.”Originality/valueThis study has identified and contributed to the limited state-of-the-art skills project managers must possess to manage major maintenance projects in the energy sector successfully. The findings would be useful to organizations within the energy sector in ensuring that the organizations have suitable personnel in place to deliver major maintenance projects on the organizations' assets.
The socio-cultural integration of immigrants in Germany: changes across generations
Authors: Giovanis Eleftherios , Akdede Sacit Hadi 
Published: December 2022

PurposePrevious studies have used language proficiency, citizenship, labour indicators, educational outcomes and political rights as measures of migrants’ socio-cultural integration. However, little is known about the migrants’ participation in volunteering activities, music concerts, theatrical plays and artistic activities, among others, and how this is compared to the participation of natives, defined as people of German descent and born in Germany. The study aims to investigate and compare the cultural and social involvement between migrants and natives.Design/methodology/approachThe analysis relies on information from the German Socio-Economic Panel Survey (GSOEP). Panel data models, in particular, the random-effects ordered Logit model, are utilised because the investigated outcomes are recorded in frequency and are ordered variables. The authors compare the participation in socio-cultural activities among immigrants of the first, second and 2.5 generations.FindingsThe findings indicate that first-generation immigrants are less likely to engage in various socio-cultural activities. However, the 2.5 generation immigrants are more active than the native population, as this generation of immigrants participates more frequently.Social implicationsThe findings highlight the importance of fostering interaction between natives and immigrants in the workplace and the social and cultural realms. Participation in social and cultural activities may increase intercultural awareness and contribute to the eradication of bias and prejudice.Originality/valueTo the best of the authors’ knowledge, this is the first study to use a large panel dataset to compare the participation of natives and migrants in various social and cultural activities. In addition, this is the first study to analyse second and 2.5-generation immigrants.
Food 4.0 for competing during the COVID-19 pandemic: experimenting digitalization in family firms
Authors: Barile Domenica , Secundo Giustina , Del Vecchio Pasquale 
Published: December 2022

PurposeWithin food industry several changes and innovations are affecting the management of the entire supply chain (production, logistics, etc.). As strategy for the survival and competition, digitalization has assumed a crucial role during the pandemic emergence by causing the reconfiguration of traditional chains and business models. Framed in these premises, the research analyses how digital technologies have innovated the sub-chains of bakery products and pasta within food industry with reference to customers' interactions, delivery and marketing during the COVID-19 pandemic emergence.Design/methodology/approachMoving from a critical literature review about the perspectives of digital technologies within the tradition of food industry, action research has been adopted to analyze in deep a case study of the start-up “ArteBianca Delivery” located in South Italy. Through this method, researchers have been deeply involved within the start-up to face the challenge of transforming the marketing and customer care into digital ones due to the COVID-19 restriction.FindingsFindings provide empirical evidence about the reconfiguration of the traditional business model of a family firm in the food sector into a digital one with the start-up “ArteBianca Delivery”. The marketing, delivery, e-commerce and customer care components of the business models have been supported and enhanced through the adoption of digital tools, such as mobile applications and social technologies useful both for users and for a more urgent digitization of company.Practical implicationsImplications for practice can be identified into the pattern of digital transformation implemented as well as in the opportunity of replication and contextualization of the results to other companies looking for setting up a digital strategy.Originality/valueElements of original contribution can be identified into: (1) the exploration of digital transformation in food family firms caused by the pandemic emergence, (2) the contextualization of the digital transformation to the sub-chains of bakery and pasta and (3) the geographical location of the case.
Purpose, process, place, pedagogical affordance and product (5Ps) of student learning in higher education: beyond Beckers
Authors: Mahat Marian 
Published: December 2022

PurposeUniversities are investing billions of dollars in building infrastructure, with the design of learning spaces driven by technological developments and long-standing changes in pedagogical theory and practice. The aim of the study is to investigate the alignment between pedagogy and space by responding to a single research question: What is the relationship between the purpose, process, place and product of student learning in higher education?Design/methodology/approachDrawing on Beckerset al.’s (2015) purpose–process–place framework, the study uses an online survey and photo elicitation method to gather perspectives from educators and students in one Australian university about their learning spaces. A hybrid approach consisting of inductive and deductive coding to thematic analysis was used to find repeated patterns of meaning.FindingsA total of 24 images of learning spaces were received from 8 educators and 16 students. Thematic analysis of the images and responses by participants highlights the importance of the pedagogical affordances of the learning environment and the product of learning, which are aligned to the purpose–process–place of learning.Research limitations/implicationsThis study provided a robust approach grounded in data to understand the alignment between space and pedagogy. Articulating students learning as an output of the alignment between space and pedagogy has important implications for the design of learning spaces and pedagogical practices in higher education. One limitation to the study, however, is noteworthy. The surveys had limited responses. Whilst the small response rates may not necessarily lead to biased results, it is acknowledged that a larger sample is likely to give more reliable results to enable the theory to be generalised.Originality/valueThe study’s findings extend the purpose–process–place framework to include pedagogical affordances and the product of learning. The purpose, process, place, pedagogical affordance and product framework posits that pedagogical affordances within a place must be deployed in such a way so that teaching and learning processes can contribute to enhancing educational goals or purpose to improve student learning outcomes, i.e. product. This framework provides a context for understanding the relationships between pedagogy and space in higher education.
M³LVI: a multi-feature, multi-metric, multi-loop, LiDAR-visual-inertial odometry via smoothing and mapping
Authors: Hu Jiaxiang , Shi Xiaojun , Ma Chunyun , Yao Xin , Wang Yingxin 
Published: December 2022

Purpose
The purpose of this paper is to propose a multi-feature, multi-metric and multi-loop tightly coupled LiDAR-visual-inertial odometry, M3LVI, for high-accuracy and robust state estimation and mapping.

Design/methodology/approach
M3LVI is built atop a factor graph and composed of two subsystems, a LiDAR-inertial system (LIS) and a visual-inertial system (VIS). LIS implements multi-feature extraction on point cloud, and then multi-metric transformation estimation is implemented to realize LiDAR odometry. LiDAR-enhanced images and IMU pre-integration have been used in VIS to realize visual odometry, providing a reliable initial guess for LIS matching module. Location recognition is performed by a dual loop module combined with Bag of Words and LiDAR-Iris to correct accumulated drift. M³LVI also functions properly when one of the subsystems failed, which greatly increases the robustness in degraded environments.

Findings
Quantitative experiments were conducted on the KITTI data set and the campus data set to evaluate the M3LVI. The experimental results show the algorithm has higher pose estimation accuracy than existing methods.

Practical implications
The proposed method can greatly improve the positioning and mapping accuracy of AGV, and has an important impact on AGV material distribution, which is one of the most important applications of industrial robots.

Originality/value
M3LVI divides the original point cloud into six types, and uses multi-metric transformation estimation to estimate the state of robot and adopts factor graph optimization model to optimize the state estimation, which improves the accuracy of pose estimation. When one subsystem fails, the other system can complete the positioning work independently, which greatly increases the robustness in degraded environments.