bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis
Authors: Klodová Božena , Potěšil David , Steinbachová Lenka , Michailidis Christos , Lindner Ann-Cathrin , Hackenberg Dieter , Becker Jörg D. , Zdráhal Zbyněk , Twell David , Honys David 
Published: October 2022

AbstractSexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.
A stochastic approach to dynamic participation in energy communities
Authors: Perger Theresia , Zwickl-Bernhard Sebastian , Golab Antonia , Auer Hans 
Published: October 2022

AbstractWith energy communities and local electricity markets on the rise, the possibilities for prosumers to be actively involved in the energy system increase, creating more complex settings for energy communities. This paper addresses the following research question: Does having knowledge about the future development in energy communities help make better decisions selecting new participants than without consideration of any future developments? Each year, the community is faced with the exit of existing members and a portfolio of possible new entrants with different characteristics. For this purpose, a bi-level optimization model for dynamic participation in local energy communities with peer-to-peer electricity trading, which is able to select the most suitable new entrants based on the preferences of the members of the original community, is extended to a stochastic dynamic program. The community wants to plan a few years ahead, which includes the following uncertainties: (i) which members leave after each period, and (ii) which are the potential new members willing to join the community. This paper’s contribution is a stochastic optimization approach to evaluate possible future developments and scenarios. The focus lies on the contractual design between the energy community and new entrants; the model calculates the duration of contracts endogenously. The results show a sample energy community’s decision-making process over a horizon of several years, comparing the stochastic approach with a simple deterministic alternative solution.
Abdominal wall hernia and mental health: patients lived experiences and implications for patient care
Authors: Smith O. A. M. , Mierzwinski M. , McVey J. , Chitsabesan P. , Chintapatla S. 
Published: October 2022

Abstract
Background
Abdominal wall hernia (AWH) affects mental health and mental health questions are frequently included within Patient-Reported Outcome Measures (PROMS) for this patient population. However, these questions have not been informed by the subjective lived experiences of mental health in AWH patients. This study is the first to qualitatively examine how AWH affects patients’ mental health.

Methods
Fifteen patients were interviewed from a purposive sample of AWH patients until no new themes emerged. Interviews explored patient thoughts and experiences of AWH and mental health. Data were examined using Interpretative Phenomenological Analysis (IPA).

Results
Three key themes pertaining to mental health were identified: “psychological and emotional distress”, “identity disruption” and “coping mechanisms and support systems”.

Conclusion
Our findings illustrate that AWH is a pathology that can have a significant detrimental impact on people’s mental health. This impact has implications for patient care and can be treated and managed through better psychological support. This support may positively affect AWH patient’s experience and outcomes in terms of quality of life. This paper provides recommendations for improved AWH patient care in regard to mental health.
Effect of balloon pre-dilation on performance of self-expandable nitinol stent in femoropopliteal artery
Authors: He Ran , Zhao Liguo , Silberschmidt Vadim V. 
Published: October 2022

AbstractBalloon pre-dilation is usually performed before implantation of a nitinol stent in a femoropopliteal artery in a case of severe blockage or calcified plaque. However, its effect on performance of the nitinol stent in a diseased femoropopliteal artery has not been studied yet. This study compares the outcomes of stenting with pre-dilation and without it by modelling the entire processes of stent deployment. Fatigue deformation of the implanted stent is also modelled under diastolic–systolic blood pressure, repetitive bending, torsion, axial compression and their combination. Reduced level of stress in the stent occurs after stenting with pre-dilation, but causing the increased damage in the media layer, i.e. the middle layer of the arterial wall. Generally, pre-dilation increases the risk of nitinol stent’s fatigue failure. Additionally, the development of in-stent restenosis is predicted based on the stenting-induced tissue damage in the media layer, and no severe mechanical irritation is induced to the media layer by pre-dilation, stent deployment or fatigue loading.
CalcGraph: taming the high costs of deep learning using models
Authors: Lorentz Joe , Hartmann Thomas , Moawad Assaad , Fouquet Francois , Aouada Djamila , Le Traon Yves 
Published: October 2022

AbstractModels based on differential programming, like deep neural networks, are well established in research and able to outperform manually coded counterparts in many applications. Today, there is a rising interest to introduce this flexible modeling to solve real-world problems. A major challenge when moving from research to application is the strict constraints on computational resources(memory and time). It is difficult to determine and contain the resource requirements of differential models, especially during the early training and hyperparameter exploration stages. In this article, we address this challenge by introducingCalcGraph, a model abstraction of differentiable programming layers.CalcGraph allows to model the computational resources that should be used and thenCalcGraph’s model interpreter can automatically schedule the execution respecting the specifications made. We propose a novel way to efficiently switch models from storage to preallocated memory zones and vice versa to maximize the number of model executions given the available resources. We demonstrate the efficiency of our approach by showing that it consumes less resources than state-of-the-art frameworks like TensorFlow and PyTorch for single-model and multi-model execution.
Neonatal seizures—diagnostic options and treatment recommendations
Authors: Ramantani Georgia , Pisani Francesco 
Published: October 2022

AbstractSeizures in neonates should prompt rapid evaluation to verify the diagnosis, determine etiology, and initiate appropriate treatment. Neonatal seizure diagnosis requires EEG confirmation and clinical observation alone is insufficient. Although most neonatal seizures are related to acute brain injury, some neonates present early-onset structural or metabolic/genetic epilepsy. Video-EEG monitoring, the gold standard for neonatal seizure detection and quantification, is resource-intensive and often unavailable, with amplitude-integrated EEG offering a reasonable alternative in guiding treatment. Whereas new-generation antiseizure medication (ASM), such as levetiracetam, appear promising, particularly in terms of tolerability, older-generation ASM, such as phenobarbital and phenytoin, are yet to be replaced. Acute treatment should aim at stopping both electroclinical and electrographic-only seizures. In neonates with acute provoked seizures, ASM should be discontinued without tapering after 72 h of seizure freedom and before hospital discharge.
With great power comes great responsibility: why ‘safe enough’ is not good enough in debates on new gene technologies
Authors: Kjeldaas Sigfrid , Dassler Tim , Antonsen Trine , Wikmark Odd-Gunnar , Myhr Anne I. 
Published: October 2022

AbstractNew genomic techniques (NGTs) are powerful technologies with the potential to change how we relate to our food, food producers, and natural environment. Their use may affect the practices and values our societies are built on. Like many countries, the EU is currently revisiting its GMO legislation to accommodate the emergence of NGTs. We argue that assessing such technologies according to whether they are ‘safe enough’ will not create the public trust necessary for societal acceptance. To avoid past mistakes of under- or miscommunication about possible impacts, we need open, transparent, and inclusive societal debate on the nature of the science of gene (editing) technologies, on how to use them, and whether they contribute to sustainable solutions to societal and environmental challenges. To be trustworthy, GMO regulation must demonstrate the authorities’ ability to manage the scientific, socio-economic, environmental, and ethical complexities and uncertainties associated with NGTs. Regulators and authorities should give equal attention to the reflexive and the emotional aspects of trust and make room for honest public and stakeholder inclusion processes. The European Group of Ethics in Science and Technology’s recent report on the Ethics of Genome Editing (2021) is important in calling attention to a series of fundamental issues that ought to be included in debates on the regulation and use of NGTs to ensure public trust in these technologies and in regulating authorities. With the great power of NGTs comes great responsibility, and the way forward must be grounded in responsible research, innovation, and regulation.
Avoidance of ant chemical traces by spider mites and its interpretation
Authors: Yano Shuichi , Konishi Mayu , Akino Toshiharu 
Published: October 2022

AbstractSpider mites become easy prey for ants when they leave their protective webs; therefore, the ability to avoid traces of ongoing ant activity should confer a selective advantage to mites. We examined avoidance of ant traces by the spider mites Tetranychus kanzawai and Tetranychus urticae. Both mite species avoided host plant leaves with active traces of Pristomyrmex punctatus or Formica japonica ants. Pristomyrmex punctatus trace avoidance by T. kanzawai lasted for more than 1 h, but not more than 3 h. Tetranychus kanzawai also avoided P. punctatus traces on plant stems, along which the mites access leaves. Moreover, T. kanzawai avoided hexane extracts of P. punctatus or F. japonica applied to a filter paper pathway. This study represents the first demonstration of a repellent effect of ant chemical traces on spider mites. Considering the substantial abundance and global distribution of ants in nature, such repellent effects may help to answer the long-standing question of why only a small fraction of available plant resources is used by herbivores. Although spider mites have developed resistance against many synthetic pesticides, natural compounds that simulate ant chemical traces may repel spider mites from agricultural crops.
LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer
Authors: Gonzalez-Morena Juan M. , Escudeiro-Lopes Sara , Ferreira-Mendes Jessica Mariane , Jakoube Pavel , Cutano Valentina , Vinaixa-Forner Judith , Kralova Viziova Petra , Hartmanova Andrea , Sedlacek Radislav , Machado Susana , Malcekova Beata , Keckesova Zuzana 
Published: October 2022

Abstract
Background
LACTB was recently identified as a mitochondrial tumour suppressor that negatively affects cancer cell proliferation by inducing cell death and/or differentiation, depending on the cell type and tissue. However, the detailed mechanism underlying the LACTB-induced cancer cell death is largely unknown.

Methods
We used cell-based, either in 2D or 3D conditions, and in vivo experiments to understand the LACTB mechanisms. In this regard, protein array followed by an enrichment analysis, cell proliferation assays using different compounds, western blot analysis, flow cytometry and immunofluorescence were performed. Differences between quantitative variables following normal distribution were valuated using Student t test for paired or no-paired samples according to the experiment. For in vivo experiments differences in tumour growth were analyzed by 2-way ANOVA.

Results
We show, that LACTB expression leads to cell cycle arrest in G1 phase and increase of DNA oxidation that leads to activation of intrinsic caspase-independent cell death pathway. This is achieved by an increase of mitochondrial reactive oxygen species since early time points of LACTB induction.

Conclusion
Our work provides a deeper mechanistic insight into LACTB-mediated cancer-cell death and shows the dynamics of the cellular responses a particular tumor suppressive stimulus might evoke under different genetic landscapes.
Variations in uniform hazard spectra and disaggregated scenarios during earthquake sequences
Authors: Azarbakht Alireza , Douglas John 
Published: October 2022

AbstractSeismic hazard varies greatly during an earthquake sequence. Understanding this variation can be useful to end-users, such as emergency managers, as it would enable them to make more informed decisions about potential risk reduction measures. This article presents examples of how two commonly-used products of probabilistic seismic hazard assessments: uniform hazard spectra and disaggregated earthquake scenarios, vary during two severe seismic sequences in western Greece. These calculations are made using a recent time-dependent seismic hazard model based on a Bayesian ETAS approach. The examples show that time-dependent uniform hazard spectra for short return periods (1 and 10 years) are significantly higher than standard time-independent spectra but that uniform hazard spectra for the commonly-used return periods of 475 and 2475 years are similar to those from time-independent assessments. The time-dependent spectra generally converge within a couple of days to the time-independent spectra. The examples also show that the dominant earthquake scenarios evidenced by the disaggregation for the time-dependent assessment can show significant differences from the time-independent scenarios. This is particularly true when the earthquake sequence is distant from the location of interest as the aftershocks contribute greatly to the overall hazard. To show these changes more clearly this article introduces a new graphical representation of the disaggregated results: contour maps showing the magnitude or distance of the dominant earthquake scenario with axes of the structural period and response spectral acceleration.
Does the Ethos of Law Erode? Lawyers’ Professional Practices, Self-Understanding and Ethics at Work
Authors: Loacker Bernadette 
Published: October 2022

AbstractFurthering an integrative ethics-as-practice framework, this paper explores the professional practices, self-understanding and ethics of lawyers working in the Germanic legal context. Existing studies of the legal profession often argue that changing conditions in law have led to a ‘constrained morality’ and an ‘erosion of ethos’ among lawyers. While the current study acknowledges shifts in lawyers’ ethos, it challenges the claim of an erosion or ‘lack’ of morality. The narratives of the interviewed practitioners rather suggest that socio-discursively constituted professional practices, identity and ethics are complex and contingent. Focusing on the ‘moral rules in use’ and how lawyers negotiate ethical matters ‘from within’ evokes ongoing ambiguities and struggles inscribed in ethical (self-)positions, pointing, as such, to the limits of assessing lawyers’ conduct as ‘ethical’or‘unethical’. The study thereby extends both normative and practice-based business and professional ethics studies.
RETRACTED ARTICLE: Comparative study of the antioxidant, toxicity, anti-inflammatory, and wound healing activities of both Digenea simplex polysaccharides and their corresponding (ZnO–Au) bimetallic nanoparticles
Authors: Abd El-Aziz Salim Mohamed , Sleem Amany A. , Abdel Maksoud M. I. A. 
Published: October 2022

AbstractThe development of green nanoparticle synthesis has multiple benefits over conventional physico-chemical techniques and provides a wide range of medical and biological applications. This study aims to synthesize zinc oxide nanoparticles (ZnONPs), gold nanoparticles (AuNPs), and bimetallic nanoparticles (BMNPs) from red algae Digenea simplex (D. simplex) polysaccharides and evaluate their antioxidant, toxicity, anti-inflammatory, and wound healing properties. High-performance liquid chromatography (HPLC) analysis was used to analyze D. simplex polysaccharides (PS). The NPs were characterized by using UV–visible (UV–vis), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM). ZnONPs showed rods and hexagonal shapes with particle sizes of 5–40 nm, AuNPs showed a spherical shape with particle sizes of 10–15 nm, and Zn-AuNPs showed a spherical shape with particle sizes of 10–40 nm. The scanning electron microscopy confirmed the presence of NPs on the cotton fabric surface in both washed and unwashed samples. The PS, ZnONPs, AuNPs and BMNPs had a good percentage of ABTS (3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, with inhibition values for ABTS of 157.1, 72.78, 78.46, and 62.81 µg/ml, and for DPPH of 364.1, 105.0, 129.9, 76.07 µg/ml respectively. The anti-inflammatory properties of BMNPs of 85.44% and ZnONPs reached 76.58% were greater than those of AuNPs of 71.51% and PS 68.98%. Furthermore, the wound healing study revealed that BMNPs have the maximum percentage reduction of 81.87%, followed by its 10 washes of 76.78%, and reached of 71.60%, 56.36%, and 51.50% for ZnONPs, AuNPs, and PS, respectively.
Asymptotic Behavior of Constrained Local Minimizers in Finite Elasticity
Authors: Mainini Edoardo , Ognibene Roberto , Percivale Danilo 
Published: October 2022

AbstractWe provide an approximation result for the pure traction problem of linearized elasticity in terms of local minimizers of finite elasticity, under the constraint of vanishing average curl for admissible deformation maps. When suitable rotations are included in the constraint, the limit is shown to be the linear elastic equilibrium associated to rotated loads.
Proton irradiation of SiPM arrays for POLAR-2
Authors: Mianowski Slawomir , De Angelis Nicolas , Hulsman Johannes , Kole Merlin , Kowalski Tomasz , Kusyk Sebastian , Li Hancheng , Mianowska Zuzanna , Mietelski Jerzy , Pollo Agnieszka , Rybka Dominik , Sun Jianchao , Swakon Jan , Wrobel Damian , Wu Xin 
Published: October 2022

AbstractPOLAR-2 is a space-borne polarimeter, built to investigate the polarization of Gamma-Ray Bursts and help elucidate their mechanisms. The instrument is targeted for launch in 2024 or 2025 aboard the China Space Station and is being developed by a collaboration between institutes from Switzerland, Germany, Poland and China. The instrument will orbit at altitudes between 340km and 450km with an inclination of $${42}{^{\circ }}$$

42

∘

and will be subjected to background radiation from cosmic rays and solar events. It is therefore pertinent to better understand the performance of sensitive devices under space-like conditions. In this paper we focus on the radiation damage of the silicon photomultiplier arrays S13361-6075NE-04 and S14161-6050HS-04 from Hamamatsu. The S13361 are irradiated with 58MeV protons at several doses up to 4.96Gy, whereas the newer series S14161 are irradiated at doses of 0.254Gy and 2.31Gy. Their respective performance degradation due to radiation damage are discussed. The equivalent exposure time in space for silicon photomultipliers inside POLAR-2 with a dose of 4.96Gy is 62.9years (or 1.78years when disregarding the shielding from the instrument). Primary characteristics of the I-V curves are an increase in the dark current and dark counts, mostly through cross-talk events. Annealing processes at $${25}{^{\circ }C}$$

25

∘

C

were observed but not studied in further detail. Biasing channels while being irradiated have not resulted in any significant impact. Activation analyses showed a dominant contribution of $$\beta ^{+}$$

β
+

particles around 511 keV. These resulted primarily from copper and carbon, mostly with decay times shorter than the orbital period.
A review of water electrolysis–based systems for hydrogen production using hybrid/solar/wind energy systems
Authors: Nasser Mohamed , Megahed Tamer F. , Ookawara Shinichi , Hassan Hamdy 
Published: October 2022

AbstractHydrogen energy, as clean and efficient energy, is considered significant support for the construction of a sustainable society in the face of global climate change and the looming energy revolution. Hydrogen is one of the most important chemical substances on earth and can be obtained through various techniques using renewable and nonrenewable energy sources. However, the necessity for a gradual transition to renewable energy sources significantly hampers efforts to identify and implement green hydrogen production paths. Therefore, this paper’s objective is to provide a technological review of the systems of hydrogen production from solar and wind energy utilizing several types of water electrolyzers. The current paper starts with a short brief about the different production techniques. A detailed comparison between water electrolyzer types and a complete illustration of hydrogen production techniques using solar and wind are presented with examples, after which an economic assessment of green hydrogen production by comparing the costs of the discussed renewable sources with other production methods. Finally, the challenges that face the mentioned production methods are illuminated in the current review.