bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of host vimentin on Eimeria tenella sporozoite invasion
Authors: Liu Zhan , Geng Xiangfei , Zhao Qiping , Zhu Shunhai , Han Hongyu , Yu Yu , Huang Wenhao , Yao Yawen , Huang Bing , Dong Hui 
Published: January 2022

AbstractBackgroundChicken coccidiosis is a parasitic disease caused byEimeriaof Apicomplexa, which has caused great economic loss to the poultry breeding industry. Host vimentin is a key protein in the process of infection of many pathogens. In an earlier phosphorylation proteomics study, we found that the phosphorylation level of host vimentin was significantly regulated afterEimeria tenellasporozoite infection. Therefore, we explored the role of host vimentin in the invasion of host cells by sporozoites.MethodsChicken vimentin protein was cloned and expressed. We used qPCR, western blotting, and indirect immunofluorescence to detect levels of mRNA transcription, translation, and phosphorylation, and changes in the distribution of vimentin afterE. tenellasporozoite infection. The sporozoite invasion rate in DF-1 cells treated with vimentin polyclonal antibody or with small interfering RNA (siRNA), which downregulated vimentin expression, was assessed by an in vitro invasion test.ResultsThe results showed that vimentin transcription and translation levels increased continually at 6–72 h afterE. tenellasporozoite infection, and the total phosphorylation levels of vimentin also changed. About 24 h after sporozoite infection, vimentin accumulated around sporozoites in DF-1 cells. Treating DF-1 cells with vimentin polyclonal antibody or downregulating vimentin expression by siRNA significantly improved the invasion efficiency of sporozoites.ConclusionIn this study, we showed that vimentin played an inhibitory role during the invasion of sporozoites. These data provided a foundation for clarifying the relationship betweenEimeriaand the host.Graphical Abstract
A discrepant presentation of bacteremia in the emergency department linked to a Fusobacterium nucleatum infection: a case report
Authors: Michel Johnny , Joly Luc-Marie , Lvovschi Virginie Eve 
Published: January 2022

Abstract
Background
Fusobacterium nucleatum is an anaerobic bacterium mainly responsible for acute or chronic infection of the ear, nose, and throat, potentially bacteremic with a risk of extraoral metastatic infection. Bacteremia occurs mainly in the elderly or in immunodeficient individuals, with high mortality. F. nucleatum is not the first cause of tonsillar infection in emergency departments, which are more often the consequence of a viral or streptococcal infection, but it is a risk factor for severe bacterial infection, especially in a viral pandemic context.

Case presentation
A 25-year-old European woman with no history presented to the emergency department with fever (38.9 °C), pharyngeal symptoms, intermittent headaches, and alteration of general condition. On examination, she presented odynophagia associated with moderate tonsillar hypertrophy, her neck was painful but flexible. A rapid diagnostic test for beta-hemolytic group streptococcus was negative. First biological analyses revealed an inflammatory syndrome with C-reactive protein of 76 mg/L. Procalcitonin was measured secondarily, and was 2.16 µg/L. Faced with discordant clinical and biological findings, a lumbar puncture was performed, which came back negative. At hour eight, hypotension was observed but corrected after filling with physiological serum. The patient was hospitalized for monitoring, based on a hypothesis of severe viral presentation. At hour 24, pyrexia confirmed this hypothesis. A spontaneous but transient improvement and no new hemodynamic event led to early discharge. At day three, she was rehospitalized for increased and continuous headaches, without hemodynamic severity. A broad-spectrum probabilistic antibiotic therapy of ceftriaxone and metronidazole was started due to first blood cultures positive for anaerobic Gram-negative bacilli, while waiting for identification of the pathogen. Three days later, F. nucleatum was identified. According to the microbiological results, antibiotic therapy was adapted with amoxicillin and clavulanic acid, and no further complications were observed during clinical or complementary examinations. The final diagnosis was a F. nucleatum oropharyngeal infection complicated by bacteremia, without metastatic spread.

Conclusion
The etiologies of tonsillar infection are not limited to benign viruses or bacteria. These should not be overlooked in emergency medicine, especially when the clinical presentation is discrepant. A combination of early bacterial investigations as blood culture and close clinical monitoring is the only safe way to detect bacteremia, especially in immunocompetent patients.
Auxiliary function-based algorithm for blind extraction of a moving speaker
Authors: Janský Jakub , Koldovský Zbyněk , Málek Jiří , Kounovský Tomáš , Čmejla Jaroslav 
Published: January 2022

AbstractIn this paper, we propose a novel algorithm for blind source extraction (BSE) of a moving acoustic source recorded by multiple microphones. The algorithm is based on independent vector extraction (IVE) where the contrast function is optimized using the auxiliary function-based technique and where the recently proposed constant separating vector (CSV) mixing model is assumed. CSV allows for movements of the extracted source within the analyzed batch of recordings. We provide a practical explanation of how the CSV model works when extracting a moving acoustic source. Then, the proposed algorithm is experimentally verified on the task of blind extraction of a moving speaker. The algorithm is compared with state-of-the-art blind methods and with an adaptive BSE algorithm which processes data in a sequential manner. The results confirm that the proposed algorithm can extract the moving speaker better than the BSE methods based on the conventional mixing model and that it achieves improved extraction accuracy than the adaptive method.
Unsung voices of technology in school education-findings using the constructivist grounded theory approach
Authors: Deepa V. , Sujatha R. , Mohan Jitendra 
Published: January 2022

AbstractTechnology adoption for school education further gained momentum during the COVID-19 pandemic. However, the challenges and strategies of children belonging to the less privileged (we use ‘privileged’ in the article to identify those enjoying a standard of living or rights as majority of people in the society) families are different from those of the children who come from socio-economically better-off (privileged) backgrounds. The purpose of this research is to explore the experiences of children with school education and using technology for learning. Past studies have highlighted the use of internet and communication technologies as a promising solution to provide quality school education in the remotest parts of the country. Previous research has also ascertained that the socio-economic status divide has no significant impact on the students’ ability to learn using technology. Children can use technology to learn irrespective of their socio-economic status and background. We conducted this exploratory qualitative study from a constructivist grounded theory perspective. A purposive sample of 14 students (9 from underprivileged and 5 from privileged family backgrounds) in the age group of 6–14 years, was used and unstructured interviews were conducted. We analysed the data using constructivist grounded theory methodology. We found that the experiences of privileged and underprivileged children differed with respect to access to internet, affordability of ICT device, quality teachers, parental support, and financial sponsorship. However, the experiences and perspectives of the children were found to be similar with respect to personal ownership of mobile phone device for unlimited time at own disposal, self-directed learning and having a trusted study advisor. The findings may be useful to policy makers and EdTech firms to build strategies and solutions for effective implementation of universal school education in the country.
On the use of ELF/VLF emissions triggered by HAARP to simulate PLHR and to study associated MLR events
Authors: Parrot Michel , Němec Frantisěk , Cohen Morris B. , Gołkowski Mark 
Published: January 2022

AbstractA spectrogram of Power Line Harmonic Radiation (PLHR) consists of a set of lines with frequency spacing corresponding exactly to 50 or 60 Hz. It is distinct from a spectrogram of Magnetospheric Line Radiation (MLR) where the lines are not equidistant and drift in frequency. PLHR and MLR propagate in the ionosphere and the magnetosphere and are recorded by ground experiments and satellites. If the source of PLHR is evident, the origin of the MLR is still under debate and the purpose of this paper is to understand how MLR lines are formed. The ELF waves triggered by High-frequency Active Auroral Research Program (HAARP) in the ionosphere are used to simulate lines (pulses of different lengths and different frequencies). Several receivers are utilized to survey the propagation of these pulses. The resulting waves are simultaneously recorded by ground-based experiments close to HAARP in Alaska, and by the low-altitude satellite DEMETER either above HAARP or its magnetically conjugate point. Six cases are presented which show that 2-hop echoes (pulses going back and forth in the magnetosphere) are very often observed. The pulses emitted by HAARP return in the Northern hemisphere with a time delay. A detailed spectral analysis shows that sidebands can be triggered and create elements with superposed frequency lines which drift in frequency during the propagation. These elements acting like quasi-periodic emissions are subjected to equatorial amplification and can trigger hooks and falling tones. At the end all these known physical processes lead to the formation of the observed MLR by HAARP pulses. It is shown that there is a tendency for the MLR frequencies of occurrence to be around 2 kHz although the exciting waves have been emitted at lower and higher frequencies.Graphical Abstract
Precise aftershock distribution of the 2019 Yamagata-oki earthquake using newly developed simple anchored-buoy ocean bottom seismometers and land seismic stations
Authors: Shinohara Masanao , Sakai Shin’ichi , Okada Tomomi , Sato Hiroshi , Yamashita Yusuke , Hino Ryota , Mochizuki Kimihiro , Akuhara Takeshi 
Published: January 2022

AbstractAn earthquake with a magnitude of 6.7 occurred in the Japan Sea off Yamagata on June 18, 2019. The mainshock had a source mechanism of reverse-fault type with a compression axis of WNW–ESE direction. Since the source area is positioned in a marine area, seafloor seismic observation is indispensable for obtaining the precise distribution of the aftershocks. The source area has a water depth of less than 100 m, and fishing activity is high. It is difficult to perform aftershock observation using ordinary free-fall pop-up type ocean bottom seismometers (OBSs). We developed a simple anchored-buoy type OBS for shallow water depths and performed the seafloor observation using this. The seafloor seismic unit had three-component seismometers and a hydrophone. Two orthogonal tiltmeters and an azimuth meter monitored the attitude of the package. For seismic observation at shallow water depth, we concluded that an anchored-buoy system would have the advantage of avoiding accidents. Our anchored-buoy OBS was based on a system used in fisheries. We deployed three anchored-buoy OBSs in the source region where the water depth was approximately 80 m on July 5, 2019, and two of the OBSs were recovered on July 13, 2019. Temporary land seismic stations with a three-component seismometer were also installed. The arrival times of P- and S-waves were read from the records of the OBSs and land stations, and we located hypocenters with correction for travel time. A preliminary location was performed using absolute travel time and final hypocenters were obtained using the double-difference method. The aftershocks were distributed at a depth range of 2.5 km to 10 km and along a plane dipping to the southeast. The plane formed by the aftershocks is consistent with the focal mechanism of the mainshock. The activity region of the aftershocks was positioned in the upper part of the upper crust. Focal mechanisms were estimated using the polarity of the first arrivals. Although many aftershocks had a reverse-fault focal mechanism similar to the focal solution of the mainshock, normal-fault type and strike–slip fault type focal mechanisms were also estimated.
Graphical Abstract
Relationship between topography, tropospheric wind, and frequency of mountain waves in the upper mesosphere over the Kanto area of Japan
Authors: Ishii Satoshi , Tomikawa Yoshihiro , Okuda Masahiro , Suzuki Hidehiko 
Published: January 2022

AbstractImaging observations of OH airglow were performed at Meiji University, Japan (35.6° N, 139.5° E), from May 2018 to December 2019. Mountainous areas are located to the west of the imager, and westerly winds are dominant in the lower atmosphere throughout the year. Mountain waves (MWs) are generated and occasionally propagate to the upper atmosphere. However, only four likely MW events were identified, which are considerably fewer than expected. There are two possible reasons for the low incidence: (1) MWs do not propagate easily to the upper mesosphere due to background wind conditions, and/or (2) the frequency of MW excitation was low around the observation site. Former possibility is found not to be a main reason to explain the frequency by assuming typical wind profiles in troposphere and upper mesosphere over Japan. Thus, frequency and spatial distribution of orographic wavy clouds were investigated by analyzing images taken by the Himawari-8 geostationary meteorological satellite in 2018. The number of days when wavy clouds were detected in the troposphere around the observation site (Kanto area) was about a quarter of that around the Tohoku area. This result indicates that frequency of over-mountain flow which is thought to be a source of excitation of MWs is low in Kanto area. We also found that the angle between the horizontal wind direction in troposphere and the orientation of the mountain ridge is a good proxy for the occurrence of orographic wavy clouds, i.e., excitation of MWs. We applied this proxy to the topography around the world to investigate regions where MWs are likely to be excited frequently throughout the year to discuss the likelihood of "MW hotspots" at various spatial scale.
Graphical Abstract
An event study on broadband electric field noises and electron distributions in the lunar wake boundary
Authors: Nishino Masaki N. , Kasahara Yoshiya , Harada Yuki , Saito Yoshifumi , Tsunakawa Hideo , Kumamoto Atsushi , Yokota Shoichiro , Takahashi Futoshi , Matsushima Masaki , Shibuya Hidetoshi , Shimizu Hisayoshi , Miyashita Yukinaga , Goto Yoshitaka , Ono Takayuki 
Published: January 2022

AbstractWave–particle interactions are fundamental processes in space plasma, and some plasma waves, including electrostatic solitary waves (ESWs), are recognised as broadband noises (BBNs) in the electric field spectral data. Spacecraft observations in recent decades have detected BBNs around the Moon, but the generation mechanism of the BBNs is not fully understood. Here, we study a wake boundary traversal with BBNs observed by Kaguya, which includes an ESW event previously reported by Hashimoto et al. Geophys Res Lett 37:L19204 10.1029/2010GL044529 (2010). Focusing on the relation between BBNs and electron pitch-angle distribution functions, we show that upward electron beams from the nightside lunar surface are effective for the generation of BBNs, in contrast to the original interpretation by Hashimoto et al. Geophys Res Lett 37:L19204 10.1029/2010GL044529 (2010) that high-energy electrons accelerated by strong ambipolar electric fields excite ESWs in the region far from the Moon. When the BBNs were observed by the Kaguya spacecraft in the wake boundary, the spacecraft’s location was magnetically connected to the nightside lunar surface, and bi-streaming electron distributions of downward-going solar wind strahl component and upward-going field-aligned beams (at $$\sim$$
∼
124 eV) were detected. The interplanetary magnetic field was dominated by a positive $$B_Z$$

B
Z

(i.e. the northward component), and strahl electrons travelled in the antiparallel direction to the interplanetary magnetic field (i.e. southward), which enabled the strahl electrons to precipitate onto the nightside lunar surface directly. The incident solar wind electrons cause negative charging of the nightside lunar surface, which generates downward electric fields that accelerate electrons from the nightside surface toward higher altitudes along the magnetic field. The bidirectional electron distribution is not a sufficient condition for the BBN generation, and the distribution of upward electron beams seems to be correlated with the BBNs. Ambipolar electric fields in the wake boundary should also contribute to the electron acceleration toward higher altitudes and further intrusion of the solar wind ions into the deeper wake. We suggest that solar wind ion intrusion into the wake boundary is also an important factor that controls the BBN generation by facilitating the influx of solar wind electrons there.
Graphical Abstract
Repeatability and reproducibility of corneal higher-order aberrations measurements after small incision lenticule extraction using the Scheimpflug-Placido topographer
Authors: Ning Rui , Gao Rongrong , Piñero David P. , Zhang Jun , Gao Qingyi , Jin Yili , Wang Yiran , Wang Chenxiao , Huang Jinhai 
Published: January 2022

Abstract
Background
To evaluate the precision of corneal higher-order aberrations measurements after small incision lenticule extraction (SMILE) using the Sirius Scheimpflug-Placido topographer (CSO, Italy).

Methods
Seventy-five eyes from 75 postoperative subjects were included in this prospective study. Three consecutive corneal aberrometric measurements were obtained with the Scheimpflug-Placido topographer by two experienced operators to assess intra- and inter-observer reproducibility. The within-subject standard deviation (Sw), test-retest repeatability (TRT) and the intraclass correlation coefficient (ICC) were calculated.

Results
For intraobserver repeatability of anterior and total corneal aberrations, all ICCs were more than 0.922, except for trefoil (0.722 to 0.768). The ICCs of total root mean square (RMS), coma Z (3, ± 1), and spherical aberration Z (4, 0) were over 0.810 while higher-order RMS, trefoil Z (3, ± 3), and astigmatism II Z (4, ± 2) were below 0.634 for posterior corneal surface aberrations. All Sw values for all types of aberrations were equal to or below 0.07 μm. Regarding interobserver reproducibility, all TRT values were no more than 0.12 μm, 0.05 μm, and 0.11 μm for anterior, posterior, and total corneal aberrations, respectively. The ICC values ranged from 0.875 to 0.989, from 0.686 to 0.976 and over 0.834 for anterior, posterior, and total corneal aberrations, respectively.

Conclusions
The repeatability of measurements of anterior and total corneal aberrations with the Sirius system in corneas after SMILE surgery was high, except for trefoil. There was some variability in posterior corneal aberrometric measurements. High reproducibility of corneal aberrometric measurements was observed between measurements of both examiners, except for trefoil, with poor to moderate reproducibility.
Smooth muscle degeneration of the mesenteric and branching veins causing ischemic enteritis: a case report
Authors: Sunakawa Taiki , Ito Nobuo , Moriyasu Ryo , Seki Nobuya , Takeuchi Daisuke , Sasahara Kotaro 
Published: January 2022

Abstract
Background
Ischemic bowel injuries are generally caused by arteriosclerosis, thromboembolism, or vasculitis. Ischemic enteritis is less common than ischemic colitis because of the rich collateral arteries of the small intestine. In the present case, smooth muscle degeneration of the mesenteric to the submucosal veins caused ischemic enteritis and small bowel obstruction.

Case presentation
An 85-year-old woman with recurrent enteritis eventually developed small bowel obstruction. We performed laparoscopic partial resection of the small intestine. The pathological findings revealed smooth muscle degeneration of the mesenteric veins that caused ischemic enteritis. Venous changes were detected not only in the injured region, but also in a part of the normal region of the resected specimen. She continued to experience some minor symptoms postoperatively; however, these symptoms subsided in a short period with medicine discontinuation.

Conclusion
This report shows the possibility that a disease causes ischemic enteritis with unique venous pathological changes and may recur postoperatively.
Two-time perforation of the ileal J-pouch 6 and 18 years after restorative proctocolectomy and ileal pouch–anal anastomosis for familial adenomatous polyposis: a case report
Authors: Shibata Kengo , Ebinuma Shota , Sakamoto Sodai , Suzuki Asami , Terasaki Yasunobu , Taketomi Akinobu 
Published: January 2022

Abstract
Background
Perforation of the ileal J-pouch after restorative proctocolectomy and ileal pouch–anal anastomosis are extremely rare. There has been no report of perforation of the ileal J-pouch occurring twice over several years. We report the first case of perforation at 6 and 18 years following restorative proctocolectomy.

Case presentation
The patient was a 52-year-old man who underwent a two-stage restorative proctocolectomy with a hand-sewn ileal J-pouch anal anastomosis due to familial adenomatous polyposis and sigmoid colon cancer at 34 years of age. At the age of 40, he underwent ileal pouch resection at its blind end, abdominal drainage, and anastomotic dilatation. The patient had a perforation of the blind end of the ileal J-pouch from increased intraluminal pressure, with anastomotic stricture and pervasive peritonitis. The patient had no symptoms for a few years; however, 18 years after the initial surgery and 12 years after the first perforation, the patient presented with severe abdominal pain. Computed tomography demonstrated pneumoperitoneum; accordingly, laparotomy was performed. Upon opening the abdominal cavity, contaminated ascites and inflammatory changes were documented involving the ileum. A 2-mm perforation involving the blind end of the ileal J-pouch was also observed and repaired, followed by temporary loop ileostomy creation. Postoperative endoscopy revealed an ulcer in the ileal J-pouch and a stricture located directly at the anastomosis.

Conclusions
The blind end of the J-pouch repeatedly perforated over the years due to recurrent anastomotic stricture. Regular surveillance is, therefore, considered necessary for the release of stricture, maintenance of anastomotic patency, and prevention of ileal J-pouch perforation.