bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the Risk of Complications During Pregnancy in Pregnant Women With Assisted Reproductive Technology: A Retrospective Study Using Registry Linkage From 2013 to 2018 in Shanghai, China.
Authors: He Mulan , Sun Xiaoxi , Wang Chunfang , Sui Yilun 
Published: January 2022

Abstract
Background: To evaluate the differences in pregnancy outcomes between assisted reproductive technology (ART) patients and natural pregnant women in Shanghai, China in the past 6 years objectively. And to assess the feasibility of the research method of registry-database linkage in mainland China. Methods: This retrospective study was conducted using registry-database linkage. A total of 8,102 pregnancies with ART and 8,096 parturients with spontaneous conception (SC) from 10 reproductive centers and 111 hospitals composed our retrospective study. The primary outcomes were the rates of obstetric complications (pregnancy-induced hypertention [PIH], gestational diabetes mellitus [GDM], placenta previa, mode of delivery, preterm birth [PTB], low birth weight [LBW], and macrosomia). The prenatal outcomes were compared between ART (n=8,102) and SC parturients (n=8,096), frozen-thawed embryo transfer (FET) (n=5513) and fresh embryo transfer (n=2589), and in vitro fertilization (IVF) (n=5144) and intracytoplasmic sperm injection (ICSI) (n=2958). We calculated odds ratios (ORs) and 95% confidence intervals (CIs). Results: The final matching rate of the target population was 92% by using registry linkage. ART resulted in a higher rate of multiple birth, PTB, LBW, cesarean section, placenta previa and GDM compared with SC in the singleton cohort. In ART patients, pregnant women with FET had a significantly higher risk of PIH than those with fresh embryo transfer (14.1% Vs 9.3%, AOR 1.526, 95% CI 1.300-1.790), but there was no difference between IVF and ICSI. FET is also related to the severity of PIH.Conclusions: ART increased the rate of complications during pregnancy, the risk and severity of PIH in patients with FET was higher than that in patients with fresh embryo transfer. The registry-database linkage study is an objective and feasible research method in mainland China.
Release Characteristics of Pb from Zn-Pb Mine Tailings Under Simulation Leaching Conditions
Authors: Akhavan Ahmad , Golchin Ahmad 
Published: January 2022

Abstract
In this paper, the results obtained from various leaching tests to determine the leaching behavior of lead from lead-zinc mine tailing under natural conditions were evaluated. In order to measure the level of toxicity of the tailings, synthetic precipitation leaching procedure (SPLP), toxicity characteristic leaching procedure (TCLP), leachate extraction procedure (LEP), and field leach test (FLT) protocols were used. The effect of liquid to solid ratio, particle size, contact time, and pH on lead release from tailings was also tested. Based on the results, the release of lead from the tailings was entirely affected by pH and amphoteric leaching behavior was observed. According to the results of the TCLP leaching protocol, the tailings categorized as hazardous material. Lead mobility index for S1 and S2 samples were 51% and 5.6%, respectively. The release of lead from the tailings was affected by the solubility process. The highest amount of lead release from S1 and S2 samples were observed in the particle size range of 0.3-0.5 mm and 0.6-1.0, mm respectively. According to the results, management of tailings and prevention of the release of lead into the environment, requires a special strategy. Controlling the amount of pore water, the size range of tailing particles in order to prevent wind erosion, prevent in situ pH changes, and acidification of tailings, and classification is essential factors in tailing management. In fact, health and diet security, climate change, safe water, accountable production, biodiversity, and ultimately the people must be considered in the planning of the authorities.
Automated Target-Driven Molecular Machine-Based Engineering Process For SARS-CoV-2 Monitoring
Authors: Fan Zhenqiang , Xie Minhao , Pan Jianbin , Zhang Kai 
Published: January 2022

Abstract
Nucleic acid structured electrochemiluminescence (ECL) biosensors provide valuable, versatile, programmable support for the development of medical diagnostics. Here, we constructed an automated DNA molecular machine with just one thiol-modified DNA probe and three nucleic acid probes on the surface of Ti3C2-based composites to monitor SARS-CoV-2, free from the limitations of motion tracks on the DNA molecular machine and significantly decreasing the probe modification fee. In the presence of the target, the designed DNA molecular machine conducted a modular reaction to transduce the target concentration information into an enhancement of the ECL signal based on DNA hybridization calculations. Modular, scalable reactions occur on DNA automata, reducing complex bioanalytical reactions to a single nucleic acid probe unit and eliminating the tedious steps of laying down motion tracks required by traditional molecular machines. By designing three capture probes, it is possible to extend the application ranges of the protocol from single to multi-target monitoring. Moreover, the strategy implements the bioanalysis of the SARS-CoV-2 in complex environments such as saliva dilutions and serum dilutions, which is valuable in promoting public health development and evaluating the environmental hazards of SARS-CoV-2.
Corneal Irregular Astigmatism And Visual Function On Anterior Segment Optical Coherence Tomography In TGFBI Corneal Dystrophy
Authors: Abe Yuito , Omoto Takashi , Kitamoto Kohdai , Toyono Tetsuya , Yoshida Junko , Asaoka Ryo , Yamagami Satoru , Miyai Takashi , Usui Tomohiko 
Published: January 2022

Abstract
The purpose of this study was to evaluate corneal irregular astigmatism of patients with granular and lattice corneal dystrophy (GCD and LCD). 70 GCD, 35 LCD, and 23 control eyes were included. Anterior and posterior corneal topographic data obtained from anterior segment optical coherence tomography were expanded into four components via Fourier harmonic analysis. These components were compared with healthy eyes and the association between each component and best-corrected visual acuity (BCVA) was investigated. Anterior and posterior components increased in LCD eyes, but none increased in GCD. Posterior components of GCD type 2 (GCD2), anterior and posterior of LCD type 1 (LCD1), posterior of LCD type IIIA (LCD 3A) and type IV (LCD4) significantly increased. BCVA was significantly associated with anterior and posterior components in LCD eyes but not in GCD. The anterior components of LCD1, anterior and posterior of LCD3A, and posterior of LCD4, but not the posterior of LCD3A, were positively correlated with BCVA. As conclusions, in GCD eyes, none of the components differed from those of the control and BCVA was not significantly associated with them. In LCD eyes, the anterior and posterior components increased, and BCVA was significantly associated with the anterior and posterior components.
Utilization of Freeze Thaw Process for Sodium Alginate and Polyvinyl Alcohol-Based Hydrogel Composite
Authors: Muangsri Rapeepan , Chuysinuan Piyachat , Thanyacharoen Thanyaluck , Techasakul Supanna , Ummartyotin Sarute 
Published: January 2022

Abstract
Abstract

Sodium alginate and poly vinyl alcohol-based hydrogel was successfully prepared by freeze thaw process. Without crosslinking agent, hydrogel was formed by using 3, 5 and 7 consecutive cycles of freeze thaw. Hydrogel can be formed by hydrogen bonding formation between OH-group of sodium alginate and poly vinyl alcohol throughout network. Scanning electron microscope reported that microstructure of hydrogel was well packed. The interconnect porosity was also observed. Differential scanning calorimetry exhibited that two exothermic peaks at temperature of 110ºC and 225ºC were observed due to evaporation of water molecule and melting temperature of hydrogel, respectively. The swelling behavior was rapidly increased within initial stage and then it was dimensionally stable. With low sodium alginate content, tensile strength was slightly superior, whereas low in vitro degradation behavior was observed. It was remarkable to note that sodium alginate and poly vinyl alcohol-based hydrogel exhibited outstanding properties for being as a medical material.
Comparison of Molecular and Morphological Identification of Ticks in Vellore District of Tamil Nadu
Authors: Saravanan Kalaimani , Aruliah Rajasekar , Prakash John A J , Kannan Kamarasu , Paramasivan Ramlingam , David Ernest 
Published: January 2022

Abstract
In this study, we have comparatively identified molecular and morphological features of ticks in Vellore district of Tamil Nadu. A total of 541 ticks were collected from domestic animals in the Vellore district of Tamil Nadu. The research was carried out between October 2018 and December 2019. The collected tick specimens were transferred into two sterile tubes and stored at room temperature in 70% alcohol. The tick was identified using PCR amplification of the 18SrRNA genes of Rhipicephalus sp (MW131877, MW318996, MW131878, MT952071) and Heamaphysalis sp (MW327046), the mtCOX1 gene of Rhipicephalus microplus (MT941478, MT941466, MT941463), and Hylomma marginatum (MW327046) (MW322564). The phylogenetic tree indicates that there was a common ancestor tick between Rhipicephalus sp., Heamaphysalis sp., and Hylomma marginatum in Vellore, Tamil Nadu. The tick was identified morphologically as two genera, Rhipicephalus sp. and Heamaphysalis sp. This is one of the first reports on ticks in the Vellore district of Tamil Nadu, as well as the morphological confirmation of two genera, Rhipicephalus and Heamaphisalis, through the sequencing of the 18SrRNA and mtCOX1 genes.
Presence and localization of apelin and its cognate receptor in the testis of the dog using immunohistochemical and molecular biology technique.
Authors: Troisi Alessandro , Dall'Aglio Cecilia , Maranesi Margherita , Orlandi Riccardo , Pastore Sara , Bazzano Marilena , Polisca Angela 
Published: January 2022

Abstract
Apelin, a member of the adipokine family, is a new endogenous peptide and it’s involved, by interacting with a specific receptor, in the control of human and laboratory animals’male reproduction. As far as we know, no data is available about the presence of the apelinergic system in dog testicles. Therefore the aim of this study was to show, for the first time, its presence and distribution in the canine testis by immunoistochemistry and molecular biology studies. For this purpose, five fertile and healthy male dogs were used and subjected to elective orchiectomy. The immunohistochemical reaction evidenced the presence of apelin and its receptor in the canine testis. In particular, apelin appeared localized in spermatids and spermatozoa with a positive signal in the “acrosomal bodies”. Regarding to the apelin receptor, a positive immunoreaction was evidenced in the cytoplasm of the cells localized in the basal portion of the seminiferous tubules, likely Sertoli’s cells, and in the cytoplasm of Leydig cells. The RT-PCR analysis showed the presence of transcripts for apelin and apelin receptor in all the samples examined. In conclusion our result allows us to hypothesize that the role of the paracrine and endocrine apelinergic system reported in laboratory animals can also be found in the dog.
Management of an Incompetent Mid-second (Mid-2nd) Trimester Absent Ectocervix: a Case Series. Cervical Amplification Pre-cerclage Insertion
Authors: Adedipe Tolu O , Akintunde Ade A , Chukwujama U.O 
Published: January 2022

Abstract
Cervical cerclage is a treatment for an incompetent cervix, the latter being a contributor to spontaneous preterm birth. There is significant difficulty with a transvaginal cerclage insertion for the absent vaginal or ecto-cervix in the mid-2nd trimester period resulting in a higher risk of late miscarriages, extremely preterm labour with subsequent neonatal morbidity and mortality. A retrospective review of cases managed by additional surgical techniques and protocols increased the likelihood of a successful insertion in this cohort of patients. This was supported by adjuvant post-operative management protocol which included vaginal progesterone use, regular infection screening and lifestyle advice.Cerclage insertion was mostly from 18- 20 weeks gestational age (GA) with an increased gestational latency of 13 gestational weeks (range 12-18) with all deliveries after 34 weeks GA. Mean gestational age at delivery was 36 weeks +1 (253 days) with a range of 241- 264 days in this small cohort of patients with minimal surgical complications and corresponding good neonatal outcomes.This technique has the potential to be a beneficial therapeutic option in management of an incompetent mid-2nd trimester absent ecto-cervix.
Investigation of the Synergist Effect Extract of Licorice and Elaeagnus Angustifolia on the Chondrogenesis of Human Adipose-derived Stem Cells on Fibrin Scaffold
Authors: Rajabi Ali , Pourentezarizarch Majid , Yadegari Maryam , Anvari Morteza , Abbasi Abulghasem , Dourtaj Hengameh , Zakizade Fatemeh , Kardan Romina , Kalbibaki Masoumeh Mozzafari 
Published: January 2022

Abstract
Background: Herbal medicines have recently taken a special place in medical treatment and research. The study was performed to evaluate the effect of E. angustifolia L and licorice extract on cartilage repair using ADSC in fibrin scaffold.Methods: In the study, stem cells were isolated from adipose tissue by mechanical and enzymatic digestion of subcutaneous adipose tissue of humans. In a DMEM-enriched medium, 1% penicillin and streptomycin were cultured in T25 flasks and incubated at 37 ° C and 5% CO2 pressure. After the third passage, the cells are implanted in fibrin scaffolds. Cells cultured in scaffolding are divided into four groups: 1- control group(medium chondrogenic(DMEM)) 2- DMEM + Lic L (25 ), 3- DMEM + E. angustifolia L (20 ), and 4 - DMEM + Lic L + E. angustifolia L . After 14 days, cell viability was assessed using the trypan blue and the gene expression was measured using the Real Time- PCR method. Results: The average cell viability in different groups is above 70%, which is acceptable.Examination of COLLX&II, AGG and SOX9 gene expression in general showed that the presence of E. angustifolia L and Lic L at the specified time was effective on gene expression (P ≤0.05).. In the concomitant use of extracts, the relative expression of genes was higher than other groups, but to a lesser extent also caused hypertrophy. Conclusions: This study confirms that Lic L and E. angustifolia L in the presence of fibrin scaffolding could be an effective method in improving cartilage lesions. Concomitant use of extracts has been more effective in gene expression as well as the viability of generation cells compared to other groups. But a small amount also causes hypertrophy as well as the expression of the coll X gene.
Primary Definitive Hemostasis for Traumatic Massive Hemoperitoneum With Hemodynamic Instability Improves Outcomes: A Single-Center, Propensity Score-Matched Analysis
Authors: Matsuda Masaki , Sawano Makoto 
Published: January 2022

Abstract
BackgroundCurrently, damage control laparotomy (DCL) is a standard operative approach for traumatic massive hemoperitoneum with hemodynamic instability. However, we have often observed unsatisfactory hemostatic effects of DCL for severe abdominal traumas, requiring definitive hemostasis (DH). We hypothesized that primary DH could improve the outcomes of hemodynamically unstable patients with traumatic massive hemoperitoneum compared with DCL.MethodsThis retrospective, single-center, observational study evaluated hemodynamically unstable patients with traumatic massive hemoperitoneum requiring surgical intervention between 2005 and 2020. The patient population was divided into two groups: a DH group and a DCL group. Propensity score matching was used to adjust for differences in baseline characteristics in the two groups, a one-to-one matched analysis using nearest-neighbor matching was performed based on the estimated propensity score of each group. The primary outcome was in-hospital mortality, and secondary outcomes were 48-hour mortality and 28-day mortality.ResultsA total of 170 patients met the inclusion criteria; 141 patients underwent primary DH, and 29 underwent DCL. In the propensity-matched analysis with 27 pairs, the DH group had significantly lower in-hospital mortality (odds ratio [OR] 0.154; 95% confidence interval (CI) 0.035 to 0.682) and 28-day mortality (OR 0.200; 95% CI 0.044 to 0.913) than the DCL group, but the 48-hour mortality did not differ significantly between the two groups (25.9% vs. 44.4%; OR 0.375; 95% CI 0.099-1.414). ConclusionsPrimary DH is an effective surgical procedure for traumatic massive hemoperitoneum with hemodynamic instability. Primary DH can be the standard surgical intervention for severe abdominal trauma.
Implementation of Team-Based Learning (TBL) in a Second Year Medical School Course: Does Prior Experience with TBL Improve the Impact of this Pedagogy?
Authors: Behling Kathryn , Carrasco Gonzalo , Gentile Matthew , Salvatore Michelle , Lopez Osvaldo 
Published: January 2022

Abstract
Background: We have shown that use of Team-based learning (TBL) in a first-year Infectious Diseases (ID) course improved final examination and course performance. Therefore, we implemented TBL in the second-year Women’s Health (WH) course to improve acquisition of course content. We hypothesized that prior experience with TBL in the first-year of medical school would lead to a strong correlation between TBL performance in the first and second years. Methods: The ID course has weekly TBL exercises that cover all course material, while the WH course has one TBL that covers a small portion of the course material. Final examination and TBL individual readiness assurance test (iRAT) scores in the ID and WH courses from three classes (n=226) were obtained with institutional review board approval. Statistical analyses were performed including comparisons of means and correlation studies. Results: Average WH iRAT scores were significantly higher than ID iRAT scores (9.19 vs. 7.40,p<0.01), and iRAT scores in both courses were highly correlated (r=0.35,p<0.01). When stratifying students based on WH course performance, in struggling students, iRAT but not final examination scores were higher in the WH course than the ID course (8.73 vs. 7.00,p<0.01 and 82.45 vs. 80.51,p>0.05, respectively). Conclusions: Our results suggest that prior experience with TBL improves TBL iRAT scores, especially in struggling students. Prior TBL experience is also associated with consistent iRAT performance between first- and second-year courses in high performing students.
The role of parental and friend’s support preventing suicide among French sexual minority adolescents
Authors: Wang Xavier , Cosquer Mireille , Zhuang Min , Ali Aminata , Falissard Bruno , Corruble Emmanuelle , Gressier Florence , Jousselme Catherine 
Published: January 2022

Abstract
Introduction: French sexual minority adolescents are at a higher risk for suicide than their heterosexual peers. However, little is known on the role of parental and friend support in French lesbian, gay and bisexual (LGB) youth. The aim of this study was to research the role of parental and friend’s support in preventing suicide attempts among LGB adolescents in France.Methods: Data was drawn from a French cross-sectional study entitled “Portrait d’Adolescents”. Chi-square and multiple logistic regression analyses were used to estimate and identify associated factors of suicide attempt in the whole sample, LGB and heterosexual subgroups. Results: An anonymous self-questionnaire was filled in by 14,265 French adolescents aged 13 to 20 in 2013. Among them, 637 (4.47%) identified as LGB. Attempted suicide was independently associated with sexual orientation (30.7% vs 10.6%; OR=2.04 [1.62-2.56]; p<0.0001). Both parental and friend’s support appeared to be a protective function in suicide attempts among the heterosexuals (OR=0.52 [0.45-0.60] and OR=0.75 [0.61-0.93]), whereas only parental support was significant in the LGB group (OR=0.51 [0.31-0.82]), independently of other variables. Conclusions: French LGB adolescents have a higher risk for suicide attempts than their heterosexual peers. Parental support was reconfirmed as a strong protective factor for suicide attempt among sexual minority adolescents. Policy Implications: The prevention efforts might be achieved by identifying within-group differences among French adolescents with different sexual orientations. The supportive role of family members should be strengthened. Positive resources and healthy support systems may literally be the effective prevention of suicide attempt.
El general intellect en bicicleta. Un análisis del trabajo de repartidores de plataformas de delivery de Buenos Aires, Argentina
Authors: Fagioli Andrea 
Published: January 2022

El artículo analiza algunos aspectos del trabajo de les repartidores de plataformas de delivery, desde un marco teórico basado en algunas formulaciones de autores que se inscriben en el así llamado marxismo postoperaísta. En particular, se retoma la manera en que ciertes autores filtran a la luz de la historia del capitalismo y extienden en su alcance el concepto marxiano de general intellect. A partir de un trabajo de campo realizado con les repartidores de Buenos Aires, el texto se enfoca principalmente en la autonomía que tienen estes en el manejo de sus tareas cotidianas, que, más allá de los cuerpos, requieren la puesta al trabajo de los requisitos específicos del Homo sapiens. La hipótesis que se plantea es que esto implica, de parte de las plataformas, un manejo estratégico de las relaciones capital-trabajo y que las plataformas se pueden volver un terreno de conflicto.
Nilai-nilai etika dan estetika dalam prosesi upacara perkawinan masyarakat Jawa: wahana pembentukan karakter mulia
Authors: Mulyana Mulyana 
Published: January 2022

Penelitian ini bertujuan untuk menjelaskan kandungan kultural dalam prosesi upacara perkawinan Jawa. Kandungan kultural yang paling mendasar adalah nilai etika dan estetikanya. Nilai etika berkaitan dengan proses dan muatan pembentukan karakter, moral, atau kepribadian orang menjadi lebih baik dan positif. Para pelaku prosesi harus mengikuti irama kedisiplinan dan karakteritik upacara pengantin Jawa. Irama prosesi dan substansi yang terkandung dalam prosesi itu sendiri menuntut tumbuhnya nilai-nilai positif etika, misalnya hormat, patuh, setia, disiplin, kerja keras, cekatan, pintar, dan tanggung jawab. Di samping pembentukan etika, prosesi ini juga menuntut dikembangkannya nilai-nilai estetika (bahasa, budaya, sosial). Pendekatan yang digunakan ialah sosiolinguistik, sosiokultural, dan dilengkapi dengan discourse analysis. Data yang digunakan berupa prosesi perkawinan Jawa dalam upacara perkawinan yang diselenggarakan oleh masyarakat Jawa di wilayah Yogyakarta. Hasil dari penelitian ialah; pertama, upacara perkawinan dapat membentuk sikap mental dan karakter yang mantap, diantaranya: disiplin, mandiri, ramah, berwibawa, dan bisa diteladani. Kedua, unsur-unsur estetika linguistik yang digunakan antara lain tembung saroja, tembung garba, yogyaswara, keratabasa, tembung entar, paribasan, bebasan, saloka, pepindhan, pralambang, purwakanthi, panambang ha-, seselan –in-, seselan –um-, tembung kawi, dan diksi religiusitas. Nilai-nilai estetika kultural dan sosial yang ditemukan antara lain nilai estetika dalam hubungan sosial, persaudaraan, dan anasir budaya Jawa (estetika rumah, busana, dan makanan). Nilai Etika dan estetika tersebut seiring dengan dinamika ekonomi, sosial, dan budaya masyarakat Jawa, yang menjadi konteks upacara perkawinan Jawa, mengalami dinamika dan perubahan-perubahan yang lentur dan akomodatif. Namun perubahan itu tidak menghilangkan substansi utama pentingnya nilai budi pekerti luhur dalam budaya perkawinan adat Jawa.