bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative analysis of extracellular vesicles (EVs) from human and feline plasma
Authors: Howard Jane , Wynne Kieran , Moldenhauer Evelin , Clarke Paul , Maguire Ciaran , Bollard Stephanie , Yin Xiaofei , Brennan Lorraine , Mooney Louise , Fitzsimons Stephen , Halasz Melinda , Aluri Ester Rani , Brougham Dermot F. , Kolch Walter , Dwyer Róisín M. , Potter Shirley , Kelly Pamela , McCann Amanda 
Published: June 2022

AbstractExtracellular vesicles (EVs) are nanoparticles found in all biological fluids, capable of transporting biological material around the body. Extensive research into the physiological role of EVs has led to the development of the Minimal Information for Studies of Extracellular Vesicles (MISEV) framework in 2018. This framework guides the standardisation of protocols in the EV field. To date, the focus has been on EVs of human origin. As comparative medicine progresses, there has been a drive to study similarities between diseases in humans and animals. To successfully research EVs in felines, we must validate the application of the MISEV guidelines in this group. EVs were isolated from the plasma of healthy humans and felines. EV characterisation was carried out according to the MISEV guidelines. Human and feline plasma showed a similar concentration of EVs, comparable expression of known EV markers and analogous particle to protein ratios. Mass spectrometry analyses showed that the proteomic signature of EVs from humans and felines were similar. Asymmetrical flow field flow fractionation, showed two distinct subpopulations of EVs isolated from human plasma, whereas only one subpopulation was isolated from feline plasma. Metabolomic profiling showed similar profiles for humans and felines. In conclusion, isolation, and characterisation of EVs from humans and felines show that MISEV2018 guidelines may also be applied to felines. Potential comparative medicine studies of EVs may provide a model for studying naturally occurring diseases in both humans and felines.
Selecting putative drought-tolerance markers in two contrasting soybeans
Authors: Toum Laila , Perez-Borroto Lucia Sandra , Peña-Malavera Andrea Natalia , Luque Catalina , Welin Bjorn , Berenstein Ariel , Fernández Do Porto Darío , Vojnov Adrian , Castagnaro Atilio Pedro , Pardo Esteban Mariano 
Published: June 2022

AbstractIdentifying high-yield genotypes under low water availability is essential for soybean climate-smart breeding. However, a major bottleneck lies in phenotyping, particularly in selecting cost-efficient markers associated with stress tolerance and yield stabilization. Here, we conducted in-depth phenotyping experiments in two soybean genotypes with contrasting drought tolerance, MUNASQA (tolerant) and TJ2049 (susceptible), to better understand soybean stress physiology and identify/statistically validate drought-tolerance and yield-stabilization traits as potential breeding markers. Firstly, at the critical reproductive stage (R5), the molecular differences between the genotype’s responses to mild water deficit were explored through massive analysis of cDNA ends (MACE)-transcriptomic and gene ontology. MUNASQA transcriptional profile, compared to TJ2049, revealed significant differences when responding to drought. Next, both genotypes were phenotyped under mild water deficit, imposed in vegetative (V3) and R5 stages, by evaluating 22 stress-response, growth, and water-use markers, which were subsequently correlated between phenological stages and with yield. Several markers showed high consistency, independent of the phenological stage, demonstrating the effectiveness of the phenotyping methodology and its possible use for early selection. Finally, these markers were classified and selected according to their cost-feasibility, statistical weight, and correlation with yield. Here, pubescence, stomatal density, and canopy temperature depression emerged as promising breeding markers for the early selection of drought-tolerant soybeans.
Cost of stay and characteristics of patients with stroke and delayed discharge for non-clinical reasons
Authors: Pellico-López Amada , Fernández-Feito Ana , Cantarero David , Herrero-Montes Manuel , Cayón-de las Cuevas Joaquín , Parás-Bravo Paula , Paz-Zulueta María 
Published: June 2022

AbstractDelayed discharge for non-clinical reasons (bed-blocking) is characteristic of pathologies associated with ageing, loss of functional capacity and dependence such as stroke. The aims of this study were to describe the costs and characteristics of cases of patients with stroke and delayed discharge for non-clinical reasons (bed-blocking) compared with cases of bed-blocking (BB) for other reasons and to assess the relationship between the length of total stay (LOS) with patient characteristics and the context of care. A descriptive cross-sectional study was conducted at a high complexity public hospital in Northern Spain (2007–2015). 443 stroke patients presented with BB. Delayed discharge increased LOS by approximately one week. The median age was 79.7 years, significantly higher than in cases of BB for other reasons. Patients with stroke and BB are usually older patients, however, when younger patients are affected, their length of stay is longer in relation to the sudden onset of the problem and the lack of adequate functional recovery resources or residential facilities for intermediate care.
Transverse oscillating bubble enhanced laser-driven betatron X-ray radiation generation
Authors: Rakowski Rafal , Zhang Ping , Jensen Kyle , Kettle Brendan , Kawamoto Tim , Banerjee Sudeep , Fruhling Colton , Golovin Grigory , Haden Daniel , Robinson Matthew S. , Umstadter Donald , Shadwick B. A. , Fuchs Matthias 
Published: June 2022

AbstractUltrafast high-brightness X-ray pulses have proven invaluable for a broad range of research. Such pulses are typically generated via synchrotron emission from relativistic electron bunches using large-scale facilities. Recently, significantly more compact X-ray sources based on laser-wakefield accelerated (LWFA) electron beams have been demonstrated. In particular, laser-driven sources, where the radiation is generated by transverse oscillations of electrons within the plasma accelerator structure (so-called betatron oscillations) can generate highly-brilliant ultrashort X-ray pulses using a comparably simple setup. Here, we experimentally demonstrate a method to markedly enhance the parameters of LWFA-driven betatron X-ray emission in a proof-of-principle experiment. We show a significant increase in the number of generated photons by specifically manipulating the amplitude of the betatron oscillations by using our novel Transverse Oscillating Bubble Enhanced Betatron Radiation scheme. We realize this through an orchestrated evolution of the temporal laser pulse shape and the accelerating plasma structure. This leads to controlled off-axis injection of electrons that perform large-amplitude collective transverse betatron oscillations, resulting in increased radiation emission. Our concept holds the promise for a method to optimize the X-ray parameters for specific applications, such as time-resolved investigations with spatial and temporal atomic resolution or advanced high-resolution imaging modalities, and the generation of X-ray beams with even higher peak and average brightness.
Retracing cyanobacteria blooms in the Baltic Sea
Authors: Löptien U. , Dietze H. 
Published: June 2022

AbstractIn late summer, massive blooms and surface scums of cyanobacteria emerge regularly in the Baltic Sea. The bacteria can produce toxins and add bioavailable nitrogen fixed from atmospheric nitrogen to an already over-fertilized system. This counteracts management efforts targeted at improving water quality. Despite their critical role, the controls on cyanobacteria blooms are not comprehensively understood yet. This limits the usability of models-based bloom forecasts and projections into our warming future. Here we add to the discussion by combining, for the first time, satellite estimates of cyanobacteria blooms with output of a high-resolution general ocean circulation model and in-situ nutrient observations. We retrace bloom origins and conditions by calculating the trajectories of respective water parcels backwards in time. In an attempt to identify drivers of bloom development, we find that blooms originate and manifest themselves predominantly offshore where conditions are more nutrient-depleted compared to more coastal environments.
A laser parameter study on enhancing proton generation from microtube foil targets
Authors: Strehlow Joseph , Kim Joohwan , Bailly-Grandvaux Mathieu , Bolaños Simon , Smith Herbie , Haid Alex , Alfonso Emmanuel L. , Aniculaesei Constantin , Chen Hui , Ditmire Todd , Donovan Michael E. , Hansen Stephanie B. , Hegelich Bjorn M. , McLean Harry S. , Quevedo Hernan J. , Spinks Michael M. , Beg Farhat N. 
Published: June 2022

AbstractThe interaction of an intense laser with a solid foil target can drive $$\sim$$
∼
TV/m electric fields, accelerating ions to MeV energies. In this study, we experimentally observe that structured targets can dramatically enhance proton acceleration in the target normal sheath acceleration regime. At the Texas Petawatt Laser facility, we compared proton acceleration from a $$1\, {\upmu }\hbox {m}$$

1

μ
m

flat Ag foil, to a fixed microtube structure 3D printed on the front side of the same foil type. A pulse length (140–450 fs) and intensity ((4–10) $$\times 10^{20}$$

×

10
20

W/cm$$^2$$

2

) study found an optimum laser configuration (140 fs, 4 $$\times 10^{20}$$

×

10
20

W/cm$$^2$$

2

), in which microtube targets increase the proton cutoff energy by 50% and the yield of highly energetic protons ($$>10$$

>
10

MeV) by a factor of 8$$\times$$
×
. When the laser intensity reaches $$10^{21}$$

10
21

W/cm$$^2$$

2

, the prepulse shutters the microtubes with an overcritical plasma, damping their performance. 2D particle-in-cell simulations are performed, with and without the preplasma profile imported, to better understand the coupling of laser energy to the microtube targets. The simulations are in qualitative agreement with the experimental results, and show that the prepulse is necessary to account for when the laser intensity is sufficiently high.
Glacier fluctuations in the northern Patagonian Andes (44°S) imply wind-modulated interhemispheric in-phase climate shifts during Termination 1
Authors: Soteres Rodrigo L. , Sagredo Esteban A. , Kaplan Michael R. , Martini Mateo A. , Moreno Patricio I. , Reynhout Scott A. , Schwartz Roseanne , Schaefer Joerg M. 
Published: June 2022

AbstractThe Last Glacial Termination (T1) featured major changes in global circulation systems that led to a shift from glacial to interglacial climate. While polar ice cores attest to an antiphased thermal pattern at millennial timescales, recent well-dated moraine records from both hemispheres suggest in-phase fluctuations in glaciers through T1, which is inconsistent with the bipolar see-saw paradigm. Here, we present a glacier chronology based on 30 new 10Be surface exposure ages from well-preserved moraines in the Lago Palena/General Vintter basin in northern Patagonia (~ 44°S). We find that the main glacier lobe underwent profound retreat after 19.7 ± 0.7 ka. This recessional trend led to the individualization of the Cerro Riñón glacier by ~ 16.3 ka, which underwent minor readvances at 15.9 ± 0.5 ka during Heinrich Stadial 1, during the Antarctic Cold Reversal with successive maxima at 13.5 ± 0.4, 13.1 ± 0.4, and 13.1 ± 0.5 ka, and a minor culmination at 12.5 ± 0.4 ka during Younger Dryas time. We conclude that fluctuations of Patagonian glaciers during T1 were controlled primarily by climate anomalies brought by shifts in the Southern Westerly Winds (SWW) locus. We posit that the global covariation of mountain glaciers during T1 was linked to variations in atmospheric CO2 (atmCO2) promoted by the interplay of the SWW-Southern Ocean system at millennial timescales.
Excessive hospitalization of patients with seizures in the Germany prehospital emergency system: a retrospective cohort study
Authors: Fuest Kristina E. , Hofberger Claudia , Lorenz Marco , Ulm Bernhard , Kanz Karl-Georg , Blobner Manfred , Schaller Stefan J. 
Published: June 2022

AbstractSeizures are a common reason for calling emergency medical services. A lack of guidelines on prehospital treatment in Germany leads to high transportation rates and reduced confidence in decision making by professionals. Our aim was to investigate the reasons for hospitalization and evaluate their necessity. A retrospective analysis of all emergency medical services records in Munich, Germany was performed in order to examine the reasons for hospitalization of patients with seizures and to evaluate their trajectory following admission to a university hospital. 8882 records were analyzed with 415 records reporting seizures (4.9%). Primary endpoint was transportation to hospital. In 380 cases (92%) patients were transported, of which 177 patients (47%) had known epilepsy; 35 patients (8%) were left at scene. Older patients and patients with higher amounts of administered medication at the scene were hospitalized significantly more often (p = 0.032 and p = 0.004, respectively). Median hospital length of stay was 1 night [IQR 1–2]. In patients with out-of-hospital seizures, high hospital transportation rates were evident, most of which could be considered as not indicated. One possible reason is the lack of guidelines in Germany, which leads to uncertainty among medical staff. This results in distress for the patients, their caregivers and consequently high costs.
Discrimination reveals reconstructability of multiplex networks from partial observations
Authors: Wu Mincheng , Chen Jiming , He Shibo , Sun Youxian , Havlin Shlomo , Gao Jianxi 
Published: June 2022

AbstractAn excellent method for predicting links in multiplex networks is reflected in its ability to reconstruct them accurately. Although link prediction methods perform well on estimating the existence probability of each potential link in monoplex networks by the set of partially observed links, we lack a mathematical tool to reconstruct the multiplex network from the observed aggregate topology and partially observed links in multiplex networks. Here, we fill this gap by developing a theoretical and computational framework that builds a probability space containing possible structures with a maximum likelihood estimation. Then, we discovered that the discrimination, an indicator quantifying differences between layers from an entropy perspective, determines the reconstructability, i.e., the accuracy of such reconstruction. This finding enables us to design the optimal strategy to allocate the set of observed links in different layers for promoting the optimal reconstruction of multiplex networks. Finally, the theoretical analyses are corroborated by empirical results from biological, social, engineered systems, and a large volume of synthetic networks.
A Systematic Review of Augmentative and Alternative Communication Interventions for Children Aged From 0 to 6 Years
Authors: Leonet Oihana , Orcasitas-Vicandi Maria , Langarika-Rocafort Argia , Mondragon Nahia Idoiaga , Etxebarrieta Gorka Roman 
Published: June 2022

Purpose:
This systematic review evaluates the latest available evidence regarding augmentative and alternative communication (AAC) interventions in children from 0 to 6 years old diagnosed with various disabilities.

Method:
A systematic search was conducted in MEDLINE (OVID), PsycINFO (EBSCO), ERIC (ProQuest), SCIELO (WOS), Teacher Reference Center (EBSCO), and Education Database (ProQuest), and studies on AAC interventions in children from 0 to 6 years old diagnosed with various disabilities were selected independently by two reviewers (A.L.-R. and N.I.M.) according to the purpose of the review.

Results:
Twenty-nine of 1,709 studies met the inclusion criteria for this review. The methodological quality of the included studies was assessed, and the characteristics and results of the studies were extracted by a descriptive analysis (O.L.S. and M.O.-V.).

Conclusion:
This analysis revealed that children with different diagnoses show improvements in expressive and receptive communication, functional communication behaviors, communication participation skills, interaction strategies, and symbol and multisymbol production and comprehension by using various AAC systems.
Shape analyticity and singular perturbations for layer potential operators
Authors: Dalla Riva Matteo , Luzzini Paolo , Musolino Paolo 
Published: June 2022

We study the effect of regular and singular domain perturbations on layer potential operators for the Laplace equation. First, we consider layer potentials supported on a diffeomorphic imageϕ(∂Ω) of a reference set ∂Ω and we present some real analyticity results for the dependence upon the mapϕ. Then we introduce a perforated domain Ω(ε) with a small hole of sizeεand we compute power series expansions that describe the layer potentials on ∂Ω(ε) when the parameterεapproximates the degenerate valueε = 0.
Intraoperative Ultrasound-Guided Excision of Non-Palpable and Palpable Breast Cancer: Systematic Review and Meta-Analysis
Authors: Banys-Paluchowski Maggie , Rubio Isabel T. , Karadeniz Cakmak Güldeniz , Esgueva Antonio , Krawczyk Natalia , Paluchowski Peter , Gruber Ines , Marx Mario , Brucker Sara Y , Bündgen Nana , Kühn Thorsten , Rody Achim , Hanker Lars , Hahn Markus 
Published: June 2022

AbstractWire-guided localization (WGL) is the most frequently used localization technique in non-palpable breast cancer (BC). However, low negative margin rates, patient discomfort, and the possibility of wire dislocation have been discussed as potential disadvantages, and re-operation due to positive margins may increase relapse risk. Intraoperative ultrasound (IOUS)-guided excision allows direct visualization of the lesion and the resection volume and reduces positive margins in palpable and non-palpable tumors. We performed a systematic review on IOUS in breast cancer and 2 meta-analyses of randomized clinical trials (RCTs). In non-palpable BC, 3 RCTs have shown higher negative margin rates in the IOUS arm compared to WGL. Meta-analysis confirmed a significant difference between IOUS and WGL in terms of positive margins favoring IOUS (risk ratio 4.34, p < 0.0001, I2 = 0%). 41 cohort studies including 3291 patients were identified, of which most reported higher negative margin and lower re-operation rates if IOUS was used. In palpable BC, IOUS was compared to palpation-guided excision in 3 RCTs. Meta-analysis showed significantly higher rates of positive margins in the palpation arm (risk ratio 2.84, p = 0.0047, I2 = 0%). In 13 cohort studies including 942 patients with palpable BC, negative margin rates were higher if IOUS was used, and tissue volumes were higher in palpation-guided cohorts in most studies. IOUS is a safe noninvasive technique for the localization of sonographically visible tumors that significantly improves margin rates in palpable and non-palpable BC. Surgeons should be encouraged to acquire ultrasound skills and participate in breast ultrasound training.
Prosthodontic Management of Immediately Extracted Teeth Using Bone Graft and Leukocyte-Platelet-Rich Fibrin: A Case Report
Authors: Sharma Ruchi , Kalra Tarun , Kumar Manjit , Bansal Ajay , Singh Ramandeep 
Published: June 2022

AbstractThe aim of this article is to present the clinical application of leukocyte-platelet-rich fibrin (L-PRF) at immediately extracted site after the implant placement. A 37-year-old male patient reported to the Department of Prosthodontics, Bhojia Dental college Budh Baddi Dist Solan Himachal Pradesh 173205 with the chief complaint of decayed teeth in the posterior region and wants a fixed prosthesis. On the day of surgery, implants were placed in freshly extracted sockets along with the bone graft and L-PRF membrane. After 6 months of healing period, implant level impressions were made and final porcelain fused to metal crowns were given to the patient.
The Role of Analytics Governance to Promote Health Care Transformation
Authors: Levy Bruce , Murphy Karen , Strevig Greg , Decker Ilia , Hanson Kirk , Puttagunta Raghuveer , Sinha Shikha , Tavares Spencer , Warsame Leyla , Vawdrey David 
Published: June 2022

Abstract
Objectives Rapid digitization in health care during the 21st century has created significant data and analytics challenges for our providers and health systems. Just as information technology (IT) governance has helped manage exploding demand for IT services and increased efficiencies, analytics governance promises to bring these same benefits to data and analytics efforts. Potential governance models exist in other industries yet have not significantly penetrated health care.
Methods and Results Geisinger has implemented analytics governance throughout our enterprise. We identified and accomplished six core goals toward the establishment of analytics governance, including developing a vision; defining the organizational structure, roles, and responsibilities; managing our data assets; implementing robust data governance; establishing standardized analytics processes; and utilizing metrics to evaluate our progress. Early outcomes include improved tracking and intelligence around data/analytics requests, decreases in duplicative data/analytics efforts, the creation of the Enterprise Analytics Hub for employees to consume data, and initial steps toward self-service analytics.
Conclusion Our experiences support the proposition that analytics governance can provide meaningful benefits to health systems. It is clear from the experiences in other industries that health systems who can best manage their data and analytics will have a significant competitive advantage. Analytics governance will also provide a proper foundation for the use of advanced analytics, machine learning, and visualization tools, and prepare our workforce to utilize these tools for the benefit of patients.