bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early biomarkers for kidney injury in heat-related illness patients: a prospective observational study at Japanese Self-Defense Force Fuji Hospital
Authors: Goto Hiroyasu , Shoda Shinichi , Nakashima Hiroyuki , Noguchi Midori , Imakiire Toshihiko , Ohshima Naoki , Kinoshita Manabu , Tomimatsu Soichi , Kumagai Hiroo 
Published: April 2022

AbstractBackgroundSince heatstroke-induced acute kidney injury (AKI) can progress to chronic kidney disease, it would be useful to detect heatstroke-induced AKI and severe heat-related illness in the early phase. We studied the epidemiology of heat-related illness among patients in the Japanese Ground Self-Defense Force and evaluated the relationship between heat-related illness severity and early urinary biomarkers for AKI.MethodsWe enrolled patients who were diagnosed with heat-related illness at the Self-Defense Force Fuji Hospital from 1 May to 30 September 2020. We compared the urinary kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), liver fatty acid–binding protein (L-FABP), N-acetyl-β-D-glucosaminidase (NAG) and β2-microglobulin levels according to the severity of heat-related illness as defined by positive scores for the Japanese Association of Acute Medicine Heatstroke Working Group (JAAM-HS-WG) criteria (0, mild; 1, moderate; ≥2, severe).ResultsOf the 44 patients, kidney injury, defined as serum creatinine (sCr) ≥1.2 mg/dL, was seen in 9 (20.5%) patients. Urinary NAG, NGAL and L-FABP levels were significantly higher in the ≥2 JAAM-HS-WG criteria group than in the 0 group. Furthermore, urinary L-FABP levels were positively correlated with sCr levels. In contrast, the urinary KIM-1 levels showed the best correlation with serum cystatin C (sCysC) among these biomarkers.ConclusionsWe conclude even mild to moderate heatstroke could lead to AKI. Urinary L-FABP is useful for detecting heatstroke-induced AKI and patients with severe heat-related illness requiring immediate treatment. Urinary KIM-1 may detect heatstroke-induced AKI in terms of sCysC, although it was not related to the severity of heat-related illness.
Transcriptome Analysis Points to BES1 as a Transducer of Strigolactone Effects on Drought Memory in Arabidopsis thaliana
Authors: Korwin Krukowski Paolo , Visentin Ivan , Russo Giulia , Minerdi Daniela , Bendahmane Abdelhafid , Schubert Andrea , Cardinale Francesca 
Published: April 2022

Abstract
Strigolactones (SLs) are carotenoid-derived phytohormones governing a wide range of physiological processes, including drought-associated stomatal closure. We have previously shown in tomato that SLs regulate the so-called after-effect of drought, whereby stomatal conductance is not completely restored for some time during recovery after a drought spell, irrespective of the water potential. To ease the elucidation of its molecular underpinnings, we investigated whether this SL effect is conserved in Arabidopsis thaliana by contrasting the physiological performances of the wild-type with SL-depleted (more axillary growth 4, max4) and insensitive (dwarf 14, d14) mutants in a drought and recovery protocol. Physiological analyses showed that SLs are important to achieve a complete after-effect in A. thaliana, while transcriptome results suggested that the SL-dependent modulation of drought responses extends to a large subset (about 4/5) of genes displaying memory transcription patterns. Among these, we show that the activation of over 30 genes related to abscisic acid metabolism and signaling strongly depends on SL signaling. Furthermore, by using promoter-enrichment tools, we identified putative cis- and trans-acting factors that may be important in the SL-dependent and SL-independent regulation of genes during drought and recovery. Finally, in order to test the accuracy of our bioinformatic prediction, we confirmed one of the most promising transcription factor candidates mediating SL signaling effects on transcriptional drought memory—BRI-EMS SUPPRESSOR1 (BES1). Our findings reveal that SLs are master regulators of Arabidopsis transcriptional memory upon drought and that this role is partially mediated by the BES1 transcription factor.
Antioxidant-enriched autologous biogel promoted diabetic wound healing by remodeling inherent posttraumatic inflammatory patterning and restoring compromised microenvironment homeostasis
Authors: Yang Yixi , Wang Le , Zhou Yonglin , He Yijun , Lin Shaozhang , Zeng Yuwei , Zhou Yunhe , Li Wei , He Zaopeng , Zhao Qi , Chen Lihao , Li Zijie , Wang Wenhao , Zhang Zhi-Yong 
Published: April 2022

Abstract
Successful wound healing depends on the reconstruction of proper tissue homeostasis, particularly in the posttraumatic inflammatory tissue microenvironment. Diabetes jeopardizes tissues’ immune homeostasis in cutaneous wounds, causing persistent chronic inflammation and cytokine dysfunction. Previously, we developed an autologous regeneration factor (ARF) technology to extract the cytokine composite from autologous tissue to restore immune homeostasis and promote wound healing. However, treatment efficacy was significantly compromised in diabetic conditions. Therefore, we proposed that a combination of melatonin and ARF, which is beneficial for proper immune homeostasis reconstruction, could be an effective treatment for diabetic wounds. Our research showed that the utilization of melatonin-mediated ARF biogel (AM gel) promoted diabetic wound regeneration at a more rapid healing rate. RNA-Seq analysis showed that AM gel treatment could restore more favorable immune tissue homeostasis with unique inflammatory patterning as a result of the diminished intensity of acute and chronic inflammation. Currently, AM gel could be a novel and promising therapeutic strategy for diabetic wounds in clinical practice through favorable immune homeostatic reconstructions in the tissue microenvironment and proper posttraumatic inflammation patterning.
CuS-PNIPAm nanoparticles with the ability to initiatively capture bacteria for photothermal treatment of infected skin
Authors: Wang Zizhen , Hou Zishuo , Wang Peiwen , Chen Fan , Luo Xianglin 
Published: April 2022

Abstract
Copper sulfide nanoparticles (CuS NPs) have shown great potential in various application fields, especially in biomedical engineering fields. CuS NPs, with the ability to actively capture and kill bacteria and without the worry of biocompatibility, will greatly expand their applications. Herein, a four-arm star thermo-sensitive polyisopropylacrylamide (4sPNIPAm) was used to modify CuS NPs (CuS-PNIPAm NPs). The obtained NPs displayed the controlled release of copper ions and higher photothermal conversion ability in comparison with contrast materials CuS-PEG NPs and CuS NPs. Aggregation of CuS-PNIPAm NPs at above 34°C resulted in capturing bacteria by forming the aggregates of NPs-bacteria. Both Staphylococcus aureus and Escherichia coli co-cultured with CuS-PNIPAm NPs were completely killed upon near-infrared irradiation in minutes. Furthermore, CuS-PNIPAm NPs were verified to be a photothermal agent without toxic effect. In in vivo experiment, the NPs effectively killed the bacteria in the wound and accelerated the process of wound repairment. Overall, photothermal treatment by CuS-PNIPAm NPs demonstrates the ability to actively capture and kill bacteria, and has a potential in the treatment of infected skin and the regeneration of skin tissues. The therapy will exert a far-reaching impact on the regeneration of stubborn chronic wounds.
Musculoskeletal ultrasound for treating rheumatoid arthritis to target—a systematic literature review
Authors: Silvagni Ettore , Zandonella Callegher Sara , Mauric Eleonora , Chiricolo Sofia , Schreiber Nikolaus , Tullio Annarita , Zabotti Alen , Scirè Carlo Alberto , Dejaco Christian , Sakellariou Garifallia 
Published: April 2022

Abstract

Objective
We aimed to systematically review the literature to retrieve evidence on the diagnostic and prognostic value of musculoskeletal ultrasound for a treat to target (T2T) approach in RA.

Methods
Eight research questions were developed addressing the role of ultrasound (including different ultrasound scores and elementary lesions) for diagnosis, monitoring and prognosis of RA. PubMed and EMBASE were searched (2005–2020). Articles on RA and reporting data on musculoskeletal ultrasound were included and extracted according to the underlying questions, and risk of bias assessed according to the study design.

Results
Out of 4632 records, 60 articles were included. Due to clinical heterogeneity, meta-analysis was not possible. Ultrasound better predicted disease relapses with respect to clinical examination in patients in remission, while both methods performed similarly in predicting response to therapy, achievement of remission and radiographic progression. Ultrasound was superior to clinical examination in diagnosing joint involvement using another imaging modality, such as magnetic resonance imaging, as reference. Limited ultrasound scores performed like more extensive evaluations for the detection of joint inflammation and for outcome prediction. Higher ultrasound scores of synovitis were linked to poor outcomes at all disease stages, but a specific cut-off distinguishing between low- and high-risk groups did not emerge.

Conclusions
These data confirm the pivotal role of ultrasound when evaluating synovial inflammation and when identifying RA patients at higher risk of relapse. Further research is needed to better define the role of ultrasound in a T2T management strategy in moderately-to-highly active RA.
Descriptive epidemiology of biliary tract cancer incidence and geographic variation in Japan
Authors: Makiuchi Takeshi , Sobue Tomotaka 
Published: April 2022

Objective
The epidemiologic features of each biliary tract cancer (BTC) subtype have not been studied and disclosed in detail. The objective of this study was to provide an up-to-date description of the epidemiologic features of BTC by subtypes, especially in terms of the geographic variation of its incidence.

Methods
We calculated the age-standardized incidence rate (ASR) of each BTC subtype at national and prefectural levels using the data from the national cancer registry in 2016 and 2017. The geographic distribution of each BTC subtype incidence was assessed by calculating the ASR ratio (ASRR) against median ASR at the prefectural level and reflecting them on the Japanese map.

Results
A total of 58 438 people diagnosed with malignant BTC were registered in the national cancer registry in 2016 and 2017 [12 497 for intrahepatic bile duct cancer (IHBDC), 16 568 for gallbladder cancer (GBC), 24 602 for extrahepatic bile duct cancer (EHBDC), 4613 for ampulla of Vater cancer (AVC) and 158 for others]. ASR was higher in men than in women for IHBDC, EHBDC and AVC, and similar between men and women for GBC. The ASR of EHBDC was approximately 2 times higher than those of the other subtypes for men and similar to that of GBC for women. The geographic distribution of ASRR was different among BTC subtypes, with larger variability in EHBDC, which was remarkably higher in the north-eastern region in both men and women.

Conclusion
The pattern of the geographic distribution of incidence in each BTC subtype was different, which suggests different etiology among subtypes.
Adjuvant chemotherapy after radical cystectomy: Do all patients who need chemotherapy after surgery actually receive it?
Authors: Croock Roy , Modai Jonathan , Avda Yuval , Shpunt Igal , Jaber Morad , Peretz Yamit , Shilo Yaniv , Leibovici Dan 
Published: April 2022

Abstract

Background
Compliance with the guideline recommendations for neoadjuvant chemotherapy in patients with muscle-invasive bladder cancer is incomplete. The adjuvant chemotherapy approach has the advantage of pathology-based decision-making, allowing for patient selection. In addition, radical surgery is not delayed and treatment-related toxicity does not impair surgical fitness. The proportion of patients who completed chemotherapy after cystectomy among those who were fit and in need of treatment were evaluated. The reasons for not completing adjuvant chemotherapy were determined.

Materials and methods
We retrospectively evaluated all patients who had undergone radical cystectomy at our center over the last 7 years. Indications for adjuvant chemotherapy included pathological T > 2, any node+, or surgical margin involvement. Only patients who were fit for chemotherapy before surgery were included in the study.

Results
Of the 52 patients with muscle-invasive bladder cancer, 14 received neoadjuvant chemotherapy or unfit for chemotherapy were excluded. Of the remaining 38 patients, 14 (37%) had bladder-confined cancers and did not require additional chemotherapy. Of the 24 patients who needed chemotherapy and were fit to receive it, 8 patients completed treatment (33%), and 3 discontinued treatment due to toxicity. Twelve patients (50%) declined chemotherapy, whereas 1 patient became unfit for chemotherapy after surgery.

Conclusions
While the adjuvant chemotherapy approach could save unnecessary treatment in 37% of patients, two-thirds of those who needed chemotherapy did not complete it. Patient refusal was the primary reason for not receiving treatment.
18F-FDG and 68Ga-FAPI-04 in the Evaluation of Aggressive Perivascular Epithelioid Cell Tumor
Authors: Zhang Zeyu , Yu Yongwei , Zhang Lu , Cheng Chao , Zuo Changjing 
Published: April 2022

Abstract
A 10-year-old girl with a right renal mass underwent 18F-FDG PET/CT and 68Ga-FAPI-04 PET/MRI for presurgical assessment of tumor invasion and malignant potential. The mass showed low 18F-FDG uptake and intense 68Ga-FAPI-04 uptake. Nephrectomy was performed, and the histopathologic diagnosis was aggressive PEComa (perivascular epithelioid cell tumor). This case showed that 68Ga-FAPI-04 PET outperformed 18F-FDG PET in detecting aggressive PEComa.
Retroperitoneal Chronic Expanding Hematoma Mimicking Malignancy on FDG PET/CT
Authors: Narita Haruki , Takenaka Junki , Watanabe Shiro , Abe Takashige , Kudo Kohsuke 
Published: April 2022

Abstract
A 49-year-old Japanese man had a chief complaint of left hypochondrium pain, and a CT scan revealed a mass in the left retroperitoneal space. CT and MRI scans revealed a hemorrhagic component and surrounding fibrous tissues. FDG PET/CT images showed increased uptake in the peripheral rim of the mass, indicating a malignant tumor. The SUVmax was 7.6. We surgically resected the mass. The pathological examination confirmed the diagnosis of chronic expanding hematoma. It is difficult to differentiate CEH from malignant tumors on imaging; this should be recognized as a diagnostic pitfall.
An 18F-FDG-PET–Positive Mucoid Impaction With Ring Enhancement on CT Mimicking Lung Cancer
Authors: Hatakeyama Nobuo , Hino Hiroyuki , Takeuch Eiji , Sakiyama Shoji , Shinohara Tsutomu 
Published: April 2022

Abstract
We encountered a case of an 18F-FDG PET–positive solitary nodule with ring enhancement on CT mimicking lung cancer in the left S6 region of an 80-year-old woman. Since transbronchial biopsy by endobronchial ultrasonography with a guide sheath could not obtain sufficient material, despite the guide sheath being placed within the lesion, the patient underwent thoracoscopic partial left S6 resection. The nodule was diagnosed as acquired cystic bronchiectasis and mucoid impaction associated with chronic inflammation. The ring-shaped enhancement and the 18F-FDG uptake may reflect angiogenesis in the cystic wall and accumulation of viable inflammatory cells in the wall and inner space, respectively.
Home-Based Pulmonary Rehabilitation in Aged Individuals With Lung Tumor After Thoracoscopic Surgery
Authors: Kuo Nai-Ying , Liu Jui-Fang , Lu Hung-I , Lo Chien-Ming , Hsien Li-Chiu , Chang Chia-Ling , Hsu Miaoju 
Published: April 2022

Background:
Objective of this study was to investigate the effect of home-based pulmonary rehabilitation in older individuals with lung tumors after video-assisted thoracoscopic surgery.

Method:
Thirty-six subjects were recruited. The control group received standard care, whereas the experimental group underwent a 6-week home-based pulmonary rehabilitation additionally. Assessments were conducted at 2, 6, and 12 weeks after discharge from the hospital.

Results:
Exercise capacity significantly improved in the rehabilitation group at 2-week follow-up. Inspiratory muscle strength and lung expansion capacity significantly improved at 2- and 6-week follow-ups.

Conclusion:
Home-based pulmonary rehabilitation could shorten the recovery time of certain physical functions.
Multidomain Integrated Exercises Decreased the Risk of Falls of Community-Dwelling Older Adults
Authors: Lee Chun-Feng , Hsiao Shih-Fen 
Published: April 2022

Aims:
To determine effects of a multidomain health promotion (MDHP) scheme for the community elderly in reducing fall risks.

Methods:
Database from older persons using the scheme was analyzed and categorized as high and low risk of fall groups retrospectively. The main measures included the 30-second chair-stand, 8-ft Up-and-Go, and single-leg stance tests. The effects of training were examined and compared across different age groups.

Results:
Balance was significantly improved in the high-risk group, especially the young-old and old-old groups.

Conclusions:
The MDHP program at least 1 hour per week for the older people was promising in reducing the risk of falls.
Impact of the additional/booster dose of COVID-19 vaccine against severe disease during the epidemic phase characterized by the predominance of the Omicron variant in Italy, December 2021 - May 2022
Authors: Branda Francesco 
Published: April 2022

AbstractVaccine effectiveness (VE) against emerging SARS-CoV-2 variants is a growing issue. The aim of this work is to evaluate the impact of the COVID-19 additional/booster dose against COVID-19-related symptoms, hospitalization, and death using surveillance data (December 2021-May 2022) from the Italian National Institute of Health (ISS). As of 18 May 2022, for people fully vaccinated with three doses, VE was 87.8% (95% confidence interval (CI): 87.6-88.0), whereas VE decreased from 71.5% (95% CI: 71.0–72.0) in those vaccinated > 120 days to 69.6% (95% CI: 68.3-70.8) within 91-120 days. Results support the importance of receiving a third dose of mRNA COVID-19 vaccine.
Enterprise healthcare physician services in Canada: an environmental scan
Authors: Spithoff Sheryl , Mogic Lana 
Published: April 2022

AbstractBackgroundEmployers in Canada are increasingly offering physician services to their employees, often through third party workplace “enterprise healthcare” platforms. To date however, little work has been done to understand this method of organizing and delivering care.ObjectiveTo understand the nature, extent and implications of enterprise healthcare physician services in Canada.MethodsWe conducted structured internet and database searches to identify enterprise healthcare platforms that provided physician services and their public websites. To answer our research question, We extracted data from company websites and linked company documents as well as information from Mergent Intellect, a web-based application with business data on Canadian companies.ResultsWe identified nine companies offering enterprise physician services to employees in Canada via 11 enterprise software platforms. According to company claims, over four million Canadian employees and their family members have access to enterprise physician services. All platforms offer virtual physician services and five also facilitate in person visits. Ten of the platforms provide primary care services and one offers only addiction medicine services. Four of the platforms offer to communicate and share information with an employee’s regular primary care provider. Five state they share aggregate or de-identified health data with employers.ConclusionsEnterprise healthcare companies provide millions of Canadian employees and their families with rapid access to virtual physician services and, in some cases, in person care. These services may disrupt continuity of care (care by the same provider over time) and pose risks to employee privacy. As other Canadians do not have access to these services, enterprise healthcare is also introducing two-tiered healthcare across Canada potentially affecting the sustainability of the public healthcare system.
A comparison of different methods for handling measurements affected by medication use
Authors: Choi Jungyeon , Dekkers Olaf M. , Cessie Saskia le 
Published: April 2022

AbstractIn epidemiological research it is common to encounter measurements affected by medication use, such as blood pressure lowered by antihypertensive drugs. When one is interested in the relation between the variables not affected by medication, ignoring medication use can cause bias. Several methods have been proposed, but the problem is often ignored or handled with generic methods, such as excluding individuals on medication or adjusting for medication use in the analysis. This study aimed to investigate methods for handling measurements affected by medication use when one is interested in the relation between the unaffected variables and to provide guidance for how to optimally handle the problem. We focused on linear regression and distinguish between the situation where the affected measurement is an exposure, confounder or outcome. In the Netherlands Epidemiology of Obesity study and in several simulated settings, we compared generic and more advanced methods, such as substituting or adding a fixed value to the treated values, regression calibration, censored normal regression, Heckman’s treatment model and multiple imputation methods. We found that often-used methods such as adjusting for medication use could result in substantial bias and that methods for handling medication use should be chosen cautiously.