bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circulating VEGF-A, TNF-α, CCL2, IL-6, and IFN-γ as biomarkers of cancer in cancer-associated anti-TIF1-γ antibody-positive dermatomyositis
Authors: Li Xiaomeng , Huang Yuan , Liu Yongmei , Yan Songxin , Li Liubing , Cheng Linlin , Li Haolong , Zhan Haoting , Zhang Fengchun , Li Yongzhe 
Published: November 2022

Abstract
Objectives
The objective of the current study was to detect plasma profiles of inflammatory cytokines for determining potential biomarkers indicating cancer presence among the anti-TIF1-γ antibody-positive dermatomyositis (DM) patients.

Methods
Twenty-seven cancer-associated anti-TIF1-γ antibody-positive DM (Cancer TIF1-γ-DM) patients were compared with 20 anti-TIF1-γ antibody-positive DM patients without cancer (Non-cancer TIF1-γ-DM) and 10 healthy controls (HC). The plasma levels of 17 cytokines were determined using the Luminex 200 system. The ability of plasma VEGF-A, TNF-α, CCL2, IL-6, and IFN-γ levels to distinguish the presence of cancer was evaluated through the area under the curve (AUC) analysis. Potential protein interactions of TIF1-γ and the five cytokines were analyzed using the STRING database.

Results
VEGF-A, TNF-α, CCL2, IL-6, and IFN-γ plasma levels were significantly higher in the Cancer TIF1-γ-DM group, especially those without any anticancer treatment, than those in the non-cancer TIF1-γ-DM and HC groups. Meanwhile, anti-TIF1-γ antibody and the five cytokines could distinguish cancer presence in anti-TIF1-γ antibody-positive DM patients. The STRING network indicated that TIF1-γ potentially interacted with the cytokines. Positive correlations of VEGF-A among CCL2, IL-6, and IFN-γ and between IFN-γ and IL-6 were observed in Cancer TIF1-γ-DM patients. VEGF-A, TNF-α, CCL2, and IL-6 were positively associated with muscle-associated enzymes among the Cancer TIF1-γ-DM patients.

Conclusion
The present study identified VEGF-A, TNF-α, CCL2, IL-6, and IFN-γ as significant potential biomarkers indicating the presence of cancer and demonstrated a more detailed cytokine profile during diagnosis. These biomarkers could provide better screening strategies and insight into the Cancer TIF1-γ-DM pathogenesis.

Key Points• VEGF-A, TNF-α, CCL2, IL-6, and IFN-γ are potential biomarkers of cancer in cancer-associated anti-TIF1-γ antibody-positive dermatomyositis.

Graphical abstract
CO2 price effects on the electricity market and greenhouse gas emissions levels: an application to the Spanish market
Authors: Arcos-Vargas A. , Núñez-Hernández F. , Ballesteros-Gallardo J. A. 
Published: November 2022

AbstractThis research proposes an empirical method to estimate the impact on the wholesale electricity market of an increase in the price of CO2 emission allowances. The current literature in this field is mainly focused on long-term simulation analyses, while this study carries out a short-term analysis with microdata from the electricity market. A higher price of CO2 implies an increase in the electricity generation costs of polluting units and therefore an increase in the price of the electricity market. When CO2 becomes more expensive, polluting electricity generators are shifted in the hourly electricity supply curve towards less competitive positions (in favour of less polluting/cheaper units). Displaced polluting units could even be taken out of the market, which would imply a reduction in CO2 emissions. These short-term movements can be reproduced with our microdata of the day-ahead electricity market –data Provided by the Spanish Market Operator (OMIE). According to our results, increases in the carbon price of 10, 20, or 30 € per ton, respectively, cause increases of 1.8%, 4.2% and 5.3% in the electricity price (year 2018), while the negative effect on emissions is relatively small. Our analysis concludes with the estimation of an ARIMA-SARIMA model that looks for the main determinants of the variations in the hourly energy prices and the carbon emissions. The estimations show that the marginal supply technology in the electricity market is important in explaining these variations.
Graphical abstract
From degrader to producer: reversing the gallic acid metabolism of Pseudomonas putida KT2440
Authors: Dias Felipe M. S. , Pantoja Raoní K. , Gomez José Gregório C. , Silva Luiziana F. 
Published: November 2022

Abstract
Gallic acid is a powerful antioxidant with multiple therapeutic applications, usually obtained from the acidic hydrolysis of tannins produced by many plants. As this process generates a considerable amount of toxic waste, the use of tannases or tannase-producing microorganisms has become a greener alternative over the last years. However, their high costs still impose some barriers for industrial scalability, requiring solutions that could be both greener and cost-effective. Since Pseudomonas putida KT2440 is a powerful degrader of gallic acid, its metabolism offers pathways that can be engineered to produce it from cheap and renewable carbon sources, such as the crude glycerol generated in biodiesel units. In this study, a synthetic operon with the heterologous genes aroG4, quiC and pobA* was developed and expressed in P. putida, based on an in silico analysis of possible metabolic routes, resulting in no production. Then, the sequences pcaHG and galTAPR were deleted from the genome of this strain to avoid the degradation of gallic acid and its main intermediate, the protocatechuic acid. This mutant was transformed with the vector containing the synthetic operon and was finally able to convert glycerol into gallic acid. Production assays in shaker showed a final concentration of 346.7 ± 0.004 mg L−1 gallic acid after 72 h.
Wildlife conservation in Myanmar: trade in wild sheep and goats for meat, medicine, and trophies, with links to China, India, and Thailand
Authors: Shepherd Chris R. , Gomez Lalita , Siriwat Penthai , Nijman Vincent 
Published: November 2022

AbstractIn Myanmar, the hunting and trade of wildlife are increasingly recognised as a major threat to the persistence of species. We here focus on the trade and conservation of wild sheep and goats (Caprinae; Antilopinae) as these species are indeed hunted and traded for a variety of reasons. Seizure reports from 2000 to 2020 and 20 visits to four wildlife markets between 1998 and 2017 resulted in records of ~ 2,000 body parts, the equivalent of ~ 1,200 wild sheep and goats. When combined with data from previous surveys conducted over the same period, the number of wild sheep and goats recorded in trade increase substantially, i.e. serow (the equivalent of 1,243 animals), goral (213 animals), takin (190 animals), blue sheep (37 animals), and Tibetan antelope (10 animals). With records from 10 out of 15 States, trade appears to be widespread and persistent over time. There was poor concordance between seizure data and trade observations, but data from various surveys are largely in agreement. The most prevalent body parts in trade were horns, followed by plates (the frontal portion of the skull with horns still attached) and heads of freshly killed animals. These parts are offered for sale both for decorations and for their purported medicinal properties. Meat, fat, and rendered oils were observed frequently but because of mixture with other wildlife, it was challenging to confirm species identify or to convert this to number of animal equivalents. Tongues and eyes were offered for sale as medicine. In order to better protect wild sheep and goats in Myanmar, it is imperative that the illegal trade in their parts is more effectively curbed than at present. This is the responsibility of both the Myanmar authorities and, given the high prevalence of trade in border towns, their international partners, including China and Thailand.
Determination of the activation energy under isothermal conditions: revisited
Authors: Arcenegui-Troya Juan , Sánchez-Jiménez Pedro Enrique , Perejón Antonio , Pérez-Maqueda Luis Allan 
Published: November 2022

AbstractThe kinetic analysis of solid-state processes aims at obtaining fundamental information that can be used for predicting the time evolution of a process within a wide range of conditions. It is an extended belief that the determination of the kinetic parameters from the analysis of curves recorded under isothermal conditions is strongly conditioned by the kinetic model used to fit the experimental data. Thus, much effort is devoted to finding the model that truly describes a process in order to calculate the kinetic parameters with accuracy. In this work, we demonstrate that the value of activation energy determined from kinetic analysis of isothermal curves is independent of the kinetic model used to fit the experimental data and, taking advantage of the underlying reason for this, a method for determining the activation energy with two isothermal curves is proposed.
Relationship between [18F]FDG PET/CT and metabolomics in patients with colorectal cancer
Authors: Imajo Masashi , Norikane Takashi , Yamamoto Yuka , Maeda Yukito , Saitoh Kaori , Kato Keiko , Soga Tomoyoshi , Okano Keiichi , Nishiyama Yoshihiro 
Published: November 2022

Abstract
Introduction
Advances in metabolomics have significantly improved cancer detection, diagnosis, treatment, and prognosis.

Objectives
To investigate the relationship between metabolic tumor volume (MTV) using 2-deoxy-2-[18F]fluoro-D-glucose (FDG) positron emission tomography (PET)/ computed tomography (CT) and metabolomics data in patients with colorectal cancer (CRC).

Methods
The metabolome in tumor tissues was analyzed using capillary electrophoresis time-of-flight mass spectrometry in 33 patients with newly diagnosed CRC who underwent FDG PET/CT before treatment and had tumor tissue post-surgery. Based on the FDG PET data, MTV was calculated and was dichotomized according to the median value, and tumors were divided into low-MTV and high-MTV tumors. Metabolomics data were compared between the low-MTV and high-MTV tumors.

Results
The levels of most glycolysis-related metabolites were not different between low-MTV and high-MTV tumors. The level of component of the initial part of the tricarboxylic acid (TCA) cycle, citrate, was significantly lower in the high-MTV tumor than in the low-MTV tumor. The TCA intermediate succinate level was significantly higher in the high-MTV tumor than in the low-MTV tumor. In contrast, the TCA intermediate fumarate level was significantly lower in the high-MTV tumor than in the low-MTV tumor. The levels of many amino acids were significantly higher in the high-MTV tumor than in the low-MTV tumor.

Conclusions
Although preliminary, these results suggest that tumors with high FDG metabolism in CRC may obtain more energy by using a reverse reaction of the TCA cycle and amino-acid metabolism. However, further research is required to clarify this relationship.
Silver nanoparticles induced testicular damage targeting NQO1 and APE1 dysregulation, apoptosis via Bax/Bcl-2 pathway, fibrosis via TGF-β/α-SMA upregulation in rats
Authors: Assar Doaa H. , Mokhbatly Abd-Allah A. , ELazab Mohamed F. Abou , Ghazy Emad W. , Gaber Ahmed A. , Elbialy Zizy I. , Hassan Ayman A. , Nabil Ahmed , Asa Samah Abou 
Published: November 2022

Abstract
In medicine, silver nanoparticles (AgNPs) are employed often. They do, however, have negative impacts, particularly on the reproductive organs. This research aimed to assess AgNP impact on the testis and the possible intracellular mechanisms to induce testicular deteriorations in rats at various concentrations and different time intervals. Sprague Dawley rats (n = 40) were allocated into four equal groups: the control one, and three other groups injected intra-peritoneally with AgNP solution 0.25, 0.5, and 1 mg/kg b.w. respectively for 15 and 30 days. Our findings revealed that AgNPs reduced body and testicular weights, estradiol (E2) and testosterone (T) hormone levels, and sperm parameters while elevating the nitric oxide and malondialdehyde levels with inhibition of reduced glutathione contents in testicular tissue. Interestingly, AgNPs significantly upregulated the testicular inducible nitric oxide synthase, B cell lymphoma 2 (Bcl-2)-associated X, transforming growth factor, and alpha-smooth muscle actin (α-SMA) expression levels. However, apurinic/apyrimidinic endo deoxyribonuclease 1 (APE1), NAD (P) H quinone dehydrogenase 1 (NQO1), and Bcl-2 expression levels were all downregulated indicating exhaustion of body antioxidant and repairing defense mechanisms in testicles in comparison with the control rats. Various histological alterations were also detected which dramatically increased in rats sacrificed after 30 days such as loss of the lining cells of seminiferous tubules with no spermatozoa and tubular irregularities associated with thickening of their basement membranes. Immunolabeling implicated in the apoptotic pathway revealed a negative expression of Bcl-2 and marked immunoreactivity for caspase-3 after 30 days of AgNP treatment in comparison to the control rats. To our knowledge, there have been no previous publications on the role of the α-SMA, APE1, and NQO1 genes in the molecular pathogenesis of AgNP testicular cytotoxicity following AgNP acute and chronic exposure.
Graphical Abstract
A multinational investigation of healthcare needs, preferences, and expectations in supportive cancer care: co-creating the LifeChamps digital platform
Authors: Marshall-McKenna Rebecca , Kotronoulas Grigorios , Kokoroskos Emmanouil , Granados Andrea Gil , Papachristou Panagiotis , Papachristou Nikolaos , Collantes Gonzalo , Petridis Georgios , Billis Antonis , Bamidis Panagiotis D. ,   on behalf of the LifeChamps consortium
Published: November 2022

Abstract

Purpose
This study is to evaluate healthcare needs, preferences, and expectations in supportive cancer care as perceived by cancer survivors, family caregivers, and healthcare professionals.

Methods
Key stakeholders consisted of cancer survivors diagnosed with breast cancer, prostate cancer, or melanoma; adult family caregivers; and healthcare professionals involved in oncology. Recruitment was via several routes, and data were collected via either online surveys or telephone interviews in Greece, Spain, Sweden, and the UK. Framework analysis was applied to the dataset.

Results
One hundred and fifty-five stakeholders participated: 70 cancer survivors, 23 family caregivers, and 62 healthcare professionals (13 clinical roles). Cancer survivors and family caregivers’ needs included information and support on practical/daily living, as frustration was apparent with the lack of follow-up services. Healthcare professionals agreed on a multidisciplinary health service with a “focus on the patient” and availability closer to home. Most healthcare professionals acknowledged that patient-reported outcomes may provide “better individualised care”. Cancer survivors and family caregivers generally felt that the digital platform would be useful for timely personalised support and aided communication. Healthcare professionals were supportive of the “proactive” functionality of the platform and the expected advantages. Anticipated challenges were integration obstacles such as workload/infrastructure and training/support in using the new technology.

Conclusions
Obtaining key stakeholders’ insights provided a foundation for action to further co-create the LifeChamps digital platform to meet needs and priorities and deliver enhanced supportive care to “older” cancer survivors.

Implications for cancer survivors
Co-creation provided insight into gaps where digital support may enhance health and well-being.
Dorsoventral Arrangement of Lateral Hypothalamus Populations in the Mouse Hypothalamus: a Prosomeric Genoarchitectonic Analysis
Authors: Diaz Carmen , de la Torre Margaret Martinez , Rubenstein John L. R. , Puelles Luis 
Published: November 2022

Abstract
The lateral hypothalamus (LH) has a heterogeneous cytoarchitectonic organization that has not been elucidated in detail. In this work, we analyzed within the framework of the prosomeric model the differential expression pattern of 59 molecular markers along the ventrodorsal dimension of the medial forebrain bundle in the mouse, considering basal and alar plate subregions of the LH. We found five basal (LH1–LH5) and four alar (LH6–LH9) molecularly distinct sectors of the LH with neuronal cell groups that correlate in topography with previously postulated alar and basal hypothalamic progenitor domains. Most peptidergic populations were restricted to one of these LH sectors though some may have dispersed into a neighboring sector. For instance, histaminergic Hdc-positive neurons were mostly contained within the basal LH3, Nts (neurotensin)- and Tac2 (tachykinin 2)-expressing cells lie strictly within LH4, Hcrt (hypocretin/orexin)-positive and Pmch (pro-melanin-concentrating hormone)-positive neurons appeared within separate LH5 subdivisions, Pnoc (prepronociceptin)-expressing cells were mainly restricted to LH6, and Sst (somatostatin)-positive cells were identified within the LH7 sector. The alar LH9 sector, a component of the Foxg1-positive telencephalo-opto-hypothalamic border region, selectively contained Satb2-expressing cells. Published studies of rodent LH subdivisions have not described the observed pattern. Our genoarchitectonic map should aid in systematic approaches to elucidate LH connectivity and function.
Metabolic response after 68Ga-PSMA-PET/CT-directed IGRT/SBRT for oligometastases prostate cancer
Authors: Gawish Ahmed , Abdullayev Nurlan , El-Arayedh Souhir , Röllich Burkard , Ochel Hans-Joachim , Brunner Thomas B. 
Published: November 2022

Abstract
Background
We used 68Ga PSMA PET/CT in the current investigation to assess the metabolic response and local control of metastasis in patients with oligometastatic prostate cancer receiving SBRT.

Materials and procedures
We performed a retrospective evaluation of the medical data of all patients with oligometastatic prostate cancer who underwent stereotactic body radiation therapy (SBRT) between 2017 and 2021. Our analysis only included medical records of patients who had SBRT for oligometastatic prostate cancer and had pre and post-SBRT 68Ga PSMA PET/CT images. Patient-related (age), disease-related (Gleason score, location of metastases), and treatment-related (factors and outcomes) data were collected from the medical files.

Results
A total of 17 patients (28 lesions) with a median age of 69 years were included in the research. A median follow-up of 16.6 months was used (range 6–36 months). The median follow-up period for 68 Ga PSMA PET/CT was 8 months (the range was 5–24 months). The median pre-treatment PSA level was 1.7 ng/mL (range 0.39–18.3 ng/mL) compared to the post-treatment PSA nadir of 0.05 ng/mL (0.02–4.57). During the follow-up period, local control was 96%, and there was a link between PSMA avidity on PET. In the treated lesions, there were no recurrences. During follow-up, none of the patients experienced toxicities of grade 3 or above.

Conclusions
SBRT is a highly successful and safe way of treating patients with oligometastatic prostate cancer. Additional research is needed to examine 68Ga PSMA PET/CT to assess further for demarcation and follow-up.
Normative Pressure Affects Attitudes Toward Pornography
Authors: Grigoropoulos Iraklis 
Published: November 2022

AbstractThis study examines Greek young women’s attitudes towards pornography and identifies specific context-related social-psychological and socio-cultural predictors of women’s pornography consumption. An exploratory cross-sectional study was conducted between September 8 and November 28, 2021, via an online survey. This study’s convenience sample consisted of female undergraduate students (N = 197) who were recruited from two universities in the northern part of Greece. A between-subject, correlational design was employed. Next, a multiple regression model was employed to predict attitudes toward pornography from the other study measures, based on the significance of the associations. Grounded on the theoretical line that individual attitudes are significantly influenced by exposure to context-specific socializing agents, this study examined whether the impact of religiosity on attitudes towards pornography would be mediated by normative pressure (social norms). This study's results identify specific context-related social-psychological and socio-cultural predictors of women’s attitudes toward pornography.
The Longer it is, the Closer One Feels: Perception of Emotional Closeness to the Partner, Relationship Duration, Sexual Activity, and Satisfaction in Married and Cohabiting Persons in Norway
Authors: Træen Bente , Kvalem Ingela Lundin 
Published: November 2022

AbstractThis study explores the connection between relationship duration and feelings of closeness in Norwegian men and women, and the association with sexual satisfaction and activity. A sample of 4160 Norwegians aged 18–89 years was enrolled from a randomly selected web panel of 11,685 Norwegians. This study focused on participants who were married or cohabiting (1432 men, 1207 women). Closeness was the highest for men and women who had been with their partner for 0−6 years. However, among those who had been with their partner for 31 years or longer, men felt closer to their partners than women. Irrespective of relationship duration, the most important factor for both men and women’s perceived closeness with their partner was general sexual satisfaction. Among men who had lived with their partner for 7−20 years and 31 years or longer, having been monogamous in life was significantly associated with “inclusion of others in the self” (IOS). Further, closeness was associated with higher intercourse frequency, lower masturbation frequency, and satisfaction with genital appearance in men who had been with their partners for 31 years or more. Intercourse frequency was significantly associated with IOS in women who had been with their partner for 0−6 years. Furthermore, in women who had been with their partner for 31 years or more, satisfaction with their own weight was important for IOS. In conclusion, men and women reported similar degrees and patterns of IOS up to the point where they had been in their relationship for more than 30 years. Thereafter, women reported feeling less close to their partners, while men’s feelings of closeness increased. This may be related to physiological, psychological, and social changes in the lives of aging men and women.