bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complexin-1 Regulated Assembly of Single Neuronal SNARE Complex Revealed by Dual-Trap Optical Tweezers
Authors: Hao Tongrui , Feng Nan , Gong Fan , Yu Yang , Liu Jiaquan , Ren Yu-Xuan 
Published: March 2022

Abstract
The dynamic assembly of the Synaptic-soluble N-ethylmaleimide-sensitive factor Attachment REceptor (SNARE) complex is crucial to the membrane fusion. Traditional ensemble studies lack the information of dynamic assembly of the protein complex. Here, we apply minute force on a tethered protein complex through dual-trap optical tweezers and study the folding dynamics of SNARE complex under mechanical force regulated by complexin-1 (CpxI). We reconstructed the clamp and facilitate functions of CpxI in vitro. Specially, the 1–83 amino acids (aa) of CpxI mainly implement the facilitate function, while the N-terminal domain (NTD) plays a dominant role. CTD is mainly related to Clamping, and separate 1-83aa and C-terminal domain (CTD) of CpxI can efficiently reconstitute the inhibitory signal identical to that the full-length CpxI functions. Our observation identifies the important chaperone role of the CpxI molecule in the dynamic assembly of SNARE complex under mechanical tension, and elucidates the specific function of each fragment of CpxI molecules in the chaperone process. Additionally, the developed technique may also be applied to study the mechanobiology and folding dynamics of single molecular protein complex.
Applicability of AC impedance method for measuring time-variant corrosion rate to cracked and crack-repaired reinforced concrete
Authors: Kanemitsu Toshinori , Takaya Satoshi 
Published: March 2022

Abstract
Purpose

Several models of the time-variant corrosion rate (CR) have been proposed to predict the service life of reinforced concrete (RC) structures based on polarization resistance (PR) monitoring. However, few of them have verified the accuracy by comparing the estimated corrosion weight loss (CWL) obtained from the PR monitoring with the actual CWL. In addition, few studies have measured the time-variant CR on crack-repaired RC specimens, and the evaluation of repair efficacy is clearly an essential step in the maintenance of the structures. This study aims to clarify the applicability of the alternating current (AC) impedance method, one of the PR methods, to cracked and crack-repaired RC.

Methods

The CRs in such RC in an accelerated corrosion environment were measured every one to three months over three years using the AC impedance method. To confirm accuracy, the corroded area and CWL of the steel bars were measured.

Results

The results clarify that the measurement area of the AC impedance method was almost equal to the corroded area, which indicates that the corrosion area should be calculated in addition to the CWL for accuracy verification. The results also show that the AC impedance method can be applied to cracked and crack-repaired RC for measuring time-variant CR.

Conclusion

The CR increases rapidly after crack initiation but becomes constant with time, and early crack repair can delay corrosion progress in cracked RC to the same extent as in uncracked concrete.
Galla chinensis exhibits anticancer activity in HPV-16 infected cervical cancer cells
Authors: Zhang XuXuan , Ma XiaoPing , Wang Zhe , Dang Hongwei , Yu Wei , Han Bo , Ren Yan 
Published: March 2022

Abstract
Background
Cervical cancer induced by the human papillomavirus (HPV) is one of the most common cancers in women in developing countries. Novel treatments for cervical cancer are focused on bioactive metabolites obtained from natural sources.
Materials and Methods
The biological effects of Galla Chinensis on HPV16 + cells were analyzed by cell scratch and plate cloning, and the cycle process was evaluated by flow cytometry; PCR, WB and immunofluorescence techniques were used to explore its mechanism
Results
It was shown that compared to a blank control group, Galla chinensis is less toxic to cervical cancer cells and exhibits prominent inhibitory effects on the growth, cloning, and migration of SiHa adherent cells. The effects of Galla chinensis also included cell cycle arrest in the S phase, and our obtained results suggest that its mechanism of action may involve the downregulation of the HPV E6 oncoprotein and the reconstruction of the p53 functional protein.
Conclusion
It is proved that Galla chinensis can effectively eliminate HPV16 + cells and inhibit the development of cervical cancer. It can down regulate oncoprotein E6 and up regulate tumor suppressor protein p53.
Effects of Non-symmetrical Current Distribution Controlled by Capacitor Placement in Loop Coils at 300 MHz MRI
Authors: Hernandez Daniel , Kim Donghyuk , Jeong Yonghwa , Nam Taewoo , Kim Kyoung-Nam 
Published: March 2022

Abstract
In this work, we present a study on the effects of the non-symmetric electrical design of radiofrequency (RF) coils for magnetic resonance imaging (MRI) operating at ultra-high frequency (UHF). A typical loop coil used in MRI consists of symmetrically distributed capacitors along the coil; this design is able to produce uniform current distributions inside the coil. However, in UHF conditions, the magnetic flux density (|B1|) field produced by this setup may exhibit field distortion, requiring a method of controlling the field distribution and improving the field intensity of the circular loop coil. The control mechanism investigated in this study is based on the position of the tuning capacitor in the circular coil. We performed comparisons against a reference coil that is based on a circular coil with four symmetrically distributed tuning capacitors. We performed electromagnetic (EM) simulations and MRI at 7 T to determine the effects of variations in the position of the capacitor and confirmed that unbalanced current distributions were created.
Robot Arm Grasping Using Learning-Based Template Matching and Self-Rotation Learning Network
Authors: Le Minh-Tri , Lien Jenn-Jier James 
Published: March 2022

Abstract
Applying deep neural network models to robot-arm grasping tasks requires the laborious and time-consuming annotation of a large number of representative examples in the training process. Accordingly, this work proposes a two-stage grasping model, in which the first stage employs learning-based template matching (LTM) algorithm for estimating the object position, and a self-rotation learning (SRL) network is then proposed to estimate the rotation angle of the grasping objects in the second stage. The LTM algorithm measures similarity between the feature maps of the search and template images which are extracted by a pre-trained model. While the SRL network performs the automatic rotation and labelling of the input data for training purposes. Therefore, the proposed model does not consume an expensive human-annotation process. The experimental results show that the proposed model obtains 92.6% when testing on 2400 pairs of the template and target images. Moreover, in performing practical grasping tasks on a NVidia Jetson TX2 developer kit, the proposed model achieves a higher accuracy (88.5%) than other grasping approaches on a split of Cornell-grasp dataset.
Liquefaction and Reliquefaction Characteristics of Cement Mixing Pile Strengthening Aeolian Sand Subgrade
Authors: Zhou Yuan , Wu Honggang , Ma Zhigang , Sun Hao 
Published: March 2022

Abstract
When the line project passes through a large area of aeolian sand, cement mixing piles are often used to reinforce the subgrade, which can effectively improve the bearing capacity of the subgrade, but its anti-liquefaction effect is worthy of in-depth evaluation. Intermittent earthquakes cause more than one liquefaction of aeolian sand subgrade, so it is worth further research on the re-liquefaction characteristics of cement mixing piles in treatment of aeolian sand subgrade. In this paper, a real-time comparative test (pile reinforced area and unreinforced area) is carried out through a shaking table, and the liquefaction and re-liquefaction characteristics of the reinforced subgrade are mainly explored. The experimental model will produce the following results under 0.4g EL Centro wave excitation:(1) The pore pressure will have multiple peaks, causing the surface of the model to float or sink. The phenomenon will be weakened by experiencing multiple earthquakes of the same level or using pile reinforcement;(2) The deeper the burial depth, the longer the time for the pore pressure to reach the maximum peak value. After multiple vibrations, the corresponding time of the shallow layer in the unreinforced area and the deep layer in the reinforced area doubles;(3) The cement mixing pile can improve the liquefaction and re-liquefaction resistance of aeolian sand foundation, and the effect is best in the shallow foundation, with an increase of more than 30%, and the deeper the burial depth, the lower the effect;(4) After the subgrade has experienced a strong earthquake, its resistance to liquefaction will be improved, but the acceleration response during the next earthquake will increase significantly.
Designing a zero-order energy transition model: a guide for creating a Starter Data Kit
Authors: Cannone Carla , Allington Lucy , Barron Karla Cervantes , Charbonnier Flora , Walker Miriam Zachau , Halloran Claire , Yeganyan Rudolf , Tan Naomi , Cullen Jonathan M , Harrison John , To Long Seng , Howells Mark 
Published: March 2022

Abstract
The Paris Agreement was signed by 192 Parties, who committed to reducing emissions. Reaching such commitments by developing national decarbonisation strategies requires significant analyses and investment. Analyses for such strategies are often delayed due to a lack of accurate and up-to-date data for creating energy transition models. The Starter Data Kits address this issue by providing open-source, zero-level country datasets to accelerate the energy planning process. There is a strong demand for replicating the process of creating Starter Data Kits because they are currently only available for 69 countries in Africa, Asia, and South America. Using an African country as an example, this paper presents the methodology to create a Starter Data Kit from data collection to the creation of tool-agnostic data repositories. The paper illustrates the steps involved, provides additional information for conducting similar work in Asia and South America, and highlights the limitations of the current version of the Starter Data Kits. Future development is proposed to expand the datasets, including new and more accurate data and new energy sectors.
4-oxo-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbohydrazide (TC) based materials; DFT, NBO analysis, Fukui function analysis, photovoltaic and solar
Authors: Sakr Mahmoud , Sherbiny Farag , El-Etrawy Abd-Allah 
Published: March 2022

Abstract
Due to numerous pharmaceutical and biological activities 4-oxo-2-thioxo-1,2,3,4-tetrahydropyrimidine-5-carbohydrazide (TC) based materials, it was important to investigate Density functional theory (DFT) calculations, natural bond orbital (NBO) analysis, molecular electrostatic potential (MESP), and local reactivity usage Fukui function for six TC derivatives compounds. DFT, NBO, MESP, and local reactivity calculations were obtained via utilizing CAM-Becke's three-parameter functional and Leee Yange Parr (CAM-B3LYP) functional and 6-311G++(2d, 2p) basis set. Optimized molecular structures for all studied compounds were obtained usage the DFT/CAM-B3LYP/6-311G++(2d, 2p) method. In addition, the highest occupied molecular orbital (HOMO), lowest unoccupied molecular orbital (LUMO), energy gap (Eg), light harvest efficiency (LHE), and open-circuit voltage (Voc) of all studied MSs are calculated and illustrated. These properties indicate that these molecular modeling structures as good candidates for utilization in organic DSSCs.
Spatial transcriptomic revealed intratumor heterogeneity and cancer stem cell enrichment in colorectal cancer metastasis
Authors: Zhou Leqi , Wen Rongbo , Yu Guanyu , Zheng Kuo , Li Zhixuan , Yu Yue , Zhu Xiaoming , Sui Jinke , Gong Haifeng , Lou Zheng , Hao Liqiang , Bai Chenguang , Yang Fu , Zhang Wei 
Published: March 2022

Abstract
Background & Aims Metastasis is the main cause of mortality in colorectal cancer (CRC) patients. Exploring the mechanisms of metastasis is of great importance in both clinical and fundamental CRC research. CRC is a highly heterogeneous disease with variable therapeutic outcomes of treatment. Approach & Results In this study, we applied spatial transcriptomics (ST) to generate a tissue-wide transcriptome from two primary colorectal cancer tissues and their matched liver metastatic tissues. Spatial RNA information showed intratumoral heterogeneity (ITH) of both primary and metastatic tissues. The comparison of gene expressions across tissues revealed an apparent enrichment of cancer stem cells (CSCs) in metastatic tissues and identified FOXD1 as a novel metastatic CSC marker. Trajectory and pseudo-time analyses revealed distinct evolutionary trajectories and a dedifferentiation-differentiation process during metastasis. CellphoneDB analysis suggested a dominant interaction of CD74-MIF with tumor cells in metastatic tissues. FOXD1 was also found to be an independent risk factor and predictor of patient survival. Conclusions Our study found ITH of primary and metastatic tissues and provides novel insights into the cellular mechanisms underlying liver metastasis of CRC and foundations for therapeutic strategies for CRC metastasis.
Sigmoid colon metastasis after radical nephrectomy for clear cell renal carcinoma: a case report and literature review
Authors: Che Hao , Li Zhenjun , Yao Yuechao , Ying Xiaojiang , Wang Zian , Chen Zhiliang , Tian GuoJiang , Lu Zengxin , Liu Xibo 
Published: March 2022

Abstract
Background: Metastasis of renal cell carcinoma most commonly occurs in the lungs, lymph nodes, bones, liver, and brain. It is rare for CCRCC to metastasize to the sigmoid colon after radical nephrectomy, which often signifies systemic metastasis and poor prognosis. Case Presentation: A 65-year-old woman was hospitalized for bloody stools, and she had a radical nephrectomy eight years ago after being diagnosed with CCRCC. The preoperative colonoscopic biopsy failed to clarify the nature of the mass, and the imaging examination suggested that the mass may be malignant. Following surgery to remove the mass, it was diagnosed as sigmoid metastatic CCRCC according to histopathology and immunohistochemistry. At present, she was in good health, and no clinical or radiological evidence of metastasis was found in her 11-month follow-up after surgery. Conclusion: Metastasis may take a long time to appear after radical nephrectomy, so it is essential to actively follow up and review patients who have undergone renal cancer surgery, and early detection and treatment of metastases remain crucial.
Clonal and Seasonal Variation of Major Essential Oil Components of Salvia fruticosa Clones
Authors: Uysal Fatma , Gölükçü Muharrem , Alan Murat , Alım Esra , Turgut Kenan 
Published: March 2022

Abstract

Anatolian sage (Salvia fruticosa MILL.) is widely used in many sectors such as food, cosmetics, and pharmaceuticals. The components in an essential oil species are important criteria to determine the usage area. This study was carried out to determine the differences in essential oil components and seasonal changes in the components of six clonally selected C-clones and one natural population. Samples were collected monthly from C-clones for two years, and essential oil ratios were determined. Assessment of all clones revealed that the time of monthly harvests had a significant effect on essential oil components. The highest β-caryophyllene content of clones was recorded in December or February and for essential oil in August. The clone and harvest time interaction caused significant differences in essential oil components, and clones reached the highest values in different months. The highest 1.8 cineole content of clones was recorded between March and June. High variation in the components, the differences in harvest times, and high correlations between components indicated that clones developed by selection have a significant production potential.
Single track formation of pure copper in directed energy deposition using a blue laser
Authors: Yamaguchi Mitsugu , Yamazaki Masamichi , Funada Yoshinori , Yachi Taisei , Saikai Ayahito , Furmoto Tatsuaki 
Published: March 2022

Abstract
Additive manufacturing (AM) techniques, including directed energy deposition (DED), have been developed and applied in the copper parts manufacturing. DED processes with conventional infrared lasers require more energy owing to the low laser absorptivity of copper. Moreover, local thermal gradients cause large residual stresses, resulting in cracks, deformation, and failure of parts. This study focused on the DED process of pure copper using a blue laser with a wavelength of 445 nm and addressed the following deficiencies. Firstly, the influence of process parameters on the geometry and surface defects of the built structure, such as meandering, distortion, and cracking, were investigated. Secondly, the chemical composition of cross-sections of the built structure was analyzed using an electron probe microanalyzer (EPMA). Finally, a prediction of surface defect-free building conditions was presented. The results indicated that the laser power directly affected the built width and penetration depth, while the built height varied with the rate of powder supplied, and that the traverse speed was inversely proportional to the built height and width. The meandering and distortion of the built structure may be attributed to changes in the melting of the powder and process instability due to obstructions in the optical path of the laser. Meanwhile, the cracks generated owing to shrinkage during solidification when the molten area was formed by laser irradiation are insufficient. A structure without defects can be obtained at a powder feed rate of 20 mg/s, laser power between 60–270 W, and traverse speeds between 10–20 mm/s.
Performance of the EULAR/ACR 2019 classification criteria for systemic lupus erythematous in monogenic lupus
Authors: Al-Mayouf Sulaiman M , Akbar Lujayn , Abdwani Reem , Ginesi Giulia , Volpi Stefano , Gattorno Marco , Bakry Reima , AlHashim Samia , Alsaleem Alhanouf 
Published: March 2022

Abstract
ObjectiveTo evaluate the application of the EULAR/ACR-2019 criteria to monogenic lupus patients and compare its performance against the SLICC-2012 criteria. Methods In a multicenter retrospective cohort study, consecutive patients with monogenic lupus from three tertiary lupus clinics were enrolled. The diagnosis of monogenic lupus was based on the expert physician’s opinion or fulfilling the SLICC-2012 criteria. All enrolled patients had genetic variants. A control group of sporadic childhood SLE (cSLE) and non-SLE patients, were included. A descriptive data analysis was conducted, and the EULAR/ACR-2019 and SLICC-2012 criteria were applied to both groups.Results Forty-nine patients with monogenic lupus with a median age at diagnosis of 6.0 (IQR 3.0-10.8) years, and 104 controls (55 patients with cSLE and 49 non-lupus patients with a median age at diagnosis of 10.0 and 5.0 respectively) were included. Forty-four (89.8%) patients with monogenic lupus fulfilled the EULAR/ACR-2019 with a mean score of 22.3±8.9. The most frequent domains were immunologic (93.9%), musculoskeletal and renal (each 57.1%), and mucocutaneous (55.1%). Fifty-four (98.2%) cSLE patients and six (12.2%) non-lupus patients met the EULAR/ACR-2019 criteria with a mean score of 22.5±9.2 and 8.5±5.2, respectively. The sensitivity of the EULAR/ACR-2019 criteria in monogenic lupus was 95.6% (95% CI: 0.8-0.9) while the specificity was 75.0% (95% CI: 0.5- 0.9). ConclusionThis is the first and largest cohort of monogenic lupus patients testing the performance of the 2019-EULAR/ACR criteria. It efficiently classifies monogenic lupus patients, irrespective of the underlying genetic variants. Further studies are needed before these criteria are adopted worldwide.
Clinical Features And Incidence Of Visual Improvement Following Systemic Antibiotic Treatment In Patients With Syphilitic Uveitis
Authors: Sittivarakul Wantanee , Aramrungroj Sukrita , Seepongphun Usanee 
Published: March 2022

Abstract
Purpose: To describe the clinical features, longitudinal pattern, and incidence rate of improvement of visual acuity (VA) following antibiotic therapy in patients with syphilitic uveitis.Methods: A total of 36 patients were included in this retrospective study from 2009 to 2020. The longitudinal patterns of mean VA values during follow-up were analyzed using a linear mixed model.Results: Most patients were men with HIV coinfection (81%) and presented with panuveitis (49%). The mean VA at baseline improved from 0.97 to 0.39 logMAR at 6 months and remained stable thereafter. The cumulative incidence of VA ≥20/25 achieved by 2 years was 70%. Receiving antibiotic therapy within four weeks of the onset of ocular symptoms (adjusted hazard ratio [aHR] 3.4, P = 0.012), absence of HIV coinfection (aHR 8.2, P<0.001), absence of neurosyphilis (aHR 6.5, P = 0.037), better presenting VA (aHR 5.0, P = 0.003), and intermediate uveitis as opposed to panuveitis (aHR 11.5, P = 0.013) were predictive of achieving VA ≥20/25.Conclusions: Men with HIV coinfection represented the majority of our patients. Visual outcomes, in response to antibiotics, were favorable. Delayed treatment, poor presenting VA, presence of HIV coinfection, and concomitant neurosyphilis decreased the likelihood of VA restoration.
InSAR-Derived Source Characteristics of Iranian Blind Reverse-Fault Earthquakes and Their Implications for Seismic Hazard Evaluation
Authors: Ghayournajarkar Nematollah , Fukushima Yo , Nakahara Hisashi 
Published: March 2022

Abstract
Investigating fault characteristics is necessary for seismic hazard evaluation and can provide useful insights for municipalities to optimize their investments in safer buildings and more resilient infrastructure. The location and the geometry of the faults, especially their relationship with sedimentary layer thickness, and the amount of stress drop are critical components when evaluating strong ground motions caused by earthquakes. Interferometric Synthetic Aperture Radar (InSAR) is a powerful tool for accurately determining fault parameters of shallow inland earthquakes and investigating their characteristics in terms of seismic hazards. In this study, based on our earlier results from InSAR data inversions conducted on five Iranian shallow blind reverse-fault earthquakes, we particularly exploited the resolving power of InSAR to investigate fault characteristics and discussed their implications for seismic hazard evaluation in Iran. For all studied Iranian earthquakes, the InSAR-derived fault was deeper than the sedimentary layer. This may indicate that these earthquakes did not rupture the entire seismogenic layer, likely leaving a significant seismic potential. Moreover, the average stress drop of the Iranian earthquakes was relatively large (13.4 MPa), which might be related to their immature blind reverse faults, leading to a possibility of generating stronger short-period ground motions, which should receive greater consideration in future seismic hazard analyses. As InSAR data can be easily accessed, this study presents a cost-effective method to provide fault parameters that can be used for the evaluation of strong ground motions caused by earthquakes, even in countries where observation networks of strong ground motions are not well developed.