bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of hypoxia-preconditioned HucMSCs on neovascularization and follicle survival in frozen/thawed human ovarian cortex transplanted to immunodeficient mice
Authors: Cheng Jiaojiao , Ruan Xiangyan , Li Yanglu , Du Juan , Jin Fengyu , Gu Muqing , Zhou Qi , Xu Xin , Yang Yu , Wang Husheng , Mueck Alfred Otto 
Published: September 2022

Abstract
Background
The massive loss of follicles in the early stage of ovarian tissue transplantation is considered a significant restriction to the efficacy of ovarian tissue cryopreservation (OTC) and transplantation (OT). The use of mesenchymal stem cells (MSCs) before transplantation of ovarian fragments shortened the hypoxic period and boosted neovascularization. Hypoxia-preconditioned MSCs can enhance the potential of angiogenesis. Can hypoxia-preconditioned human umbilical cord mesenchymal stem cell (HucMSCs) and ovarian tissue co-xenotransplantation improve more neovascularization and subsequently more follicle survival in human ovarian tissue?

Methods
Frozen-thawed cortical pieces from 4 patients were transplanted into the bilateral renal capsule of immune-deficient nude mice without HucMSCs or normoxia/hypoxia-preconditioned HucMSCs. Sixty-four mice were randomly distributed into 4 groups. In each group, the mice were euthanized for blood and/or graft retrieval on post-transplantation days 3 (n = 8) and 7 (n = 8), respectively. Non-grafted frozen-thawed ovarian fragment was taken for non-grafted control. Grafts were histologically processed and analysed for follicle density and atretic follicles by HE, neovascularization by CD34 and CD31 immunohistochemical staining, primordial follicle growth by Ki67 staining, and apoptosis of stromal cell and follicles by immunofluorescence using TUNEL. The ROS and TAC levels of grafted and non-grafted tissue were assessed. We evaluated the protein expression of HIF1α, VEGFA, pAkt, Akt, and GDF9 in grafted and non-grafted ovarian tissue. E2, Prog, AMH, and FSH levels in the plasma of mice were measured after 3 and 7 days of OT.

Results
Hypoxia-preconditioned HucMSCs positively protect the grafted ovarian tissue by significantly decreasing the apoptosis and increasing higher expression of CD31, CD34, and VEGFA for earlier angiogenesis. They are crucial to preserving the resting primordial follicle pool by modulation of follicle death.

Conclusion
This is the first study to demonstrate that co-transplantation of hypoxia-preconditioned HucMSC with ovarian tissue improved earlier vascularization of ovarian grafts in the early post-grafting period, which correlates with increased follicle survival and reduced apoptosis. The HIF1α/VEGFA signal pathways may play an important role in elucidating the mechanisms of action of hypoxia-preconditioned HucMSCs with regard to OT and clinical implementation.
Construction of tandem diabody (IL-6/CD20)-secreting human umbilical cord mesenchymal stem cells and its experimental treatment on diffuse large B cell lymphoma
Authors: Zhang Jiayi , Zhong Minglu , Zhong Weijie , Lan Yanfei , Yuan Zhaohu , Duan Yuyou , Wei Yaming 
Published: September 2022

Abstract
Background
More than 40% patients with diffuse large B cell lymphoma (DLBCL) experienced relapse or refractory (R/R) lymphoma after the standard first R-CHOP therapy. IL-6 was reportedly associated with chemotherapy resistance of rituximab. Further, mesenchymal stem cells (MSCs) are known as the potential cell vehicle for their tropism toward tumor. A MSCs-based tandem diabody for treating DLBCL is currently lacking.

Methods
We constructed a tandem diabody (Tandab(IL-6/CD20)) with modified umbilical cord MSCs (UCMSCs) and designed a cell-based Tandab releasing system. Western blot, qPCR and immunofluorescence were used to confirm the construction and expression of lentivirus-infected UCMSCs. The vitality, apoptosis and homing abilities of UCMSCs were examined via CCK-8 assay, apoptosis, wound healing and migration analysis. Cell binding assay was used to demonstrate the targeting property of Tandab binding to CD20-positive DLBCL cells. Furthermore, we evaluated the viability of SU-DHL-2 and SU-DHL-4 by using CCK-8 and EDU assay after the treatment of UCMSCs-Tandab(IL-6/CD20).

Results
Tandab protein peaked at 6273 ± 487 pg/ml in the medium on day 7 after cell culture. The proliferation and homing ability of UCMSCs did not attenuate after genetically modification. Immunofluorescence images indicated the Tandab protein bound to the lymphoma cells. UCMSCs-Tandab(IL-6/CD20) inhibited the growth of SU-DHL-2 or SU-DHL-4 cells in vitro.

Conclusions
UCMSCs-Tandab(IL-6/CD20), which bound with both tumor-associated surface antigens and pro-tumor cytokines in tumor microenvironment, might serve as a potential treatment for DLBCL, evidenced by inhibiting the growth of SU-DHL-2 or SU-DHL-4 cells.
PEX5R/Trip8b-HCN2 channel regulating neuroinflammation involved in perioperative neurocognitive disorders
Authors: Xu Feng , Wang Yafeng , Han Linlin , Deng Daling , Ding Yuanyuan , Ma LuLin , Zhang Qingtong , Chen Xiangdong 
Published: September 2022

Abstract
Background
Clinical and animal studies demonstrated that neuroinflammation from anesthesia (sevoflurane) is the main contributor to cause perioperative neurocognitive disorders (PND). Recently, it was reported that microglia respond to hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which was the target of sevoflurane. Whether HCN channels are involved in the induction of neuroinflammation after sevoflurane exposure is still unclear.

Results
Sevoflurane exposure had increased cognitive dysfunction and anxiety-like behaviors in rats. Rats inhaled with sevoflurane had activated microglia and increased neuroinflammation (IL-1β, IL-6, and TNF-α) in the hippocampus. RNA sequencing identified 132 DEGs (86 up-regulated and 46 down-regulated DEGs [differentially expressed genes]) in the hippocampus of PND rats. RNA-sequencing also uncovered that sevoflurane exposure down-regulates HCN2 expression. Pathway and process enrichment analysis suggests DEGs are mainly enriched in regulation of system process, positive regulation of glutamate secretion, secretion, regulation of synaptic transmission, regulation of nervous system process, behavior, negative regulation of sodium ion transport, and learning or memory. We validated that sevoflurane exposure can down-regulate the levels of PEX5R/Trip8b (an interaction partner and auxiliary subunit of HCN channels) and HCN1-4 channels in the hippocampus of PND rats. We used immunofluorescence staining to identify that HCN2 co-labels with neurons (Neun), astrocytes (GFAP), and microglia (iba1). We observed that the co-labeling of HCN2 with neurons or microglia decreased in the hippocampus and cortex after sevoflurane exposure. Blocking HCN2 by ZD7288 treatment further activated microglia and aggravated sevoflurane exposure-induced anxiety-like behavior, cognitive impairment, and neuroinflammation.

Conclusions
We concluded that sevoflurane exposure can induce an increased level of neuroinflammation, microglial activation, cognitive dysfunction, and anxiety-like behaviors in rats. HCN2 channel, as the target of sevoflurane action, mediates this process. HCN2 might be a target for the treatment and prevention of sevoflurane-induced PND.
Positioning and power optimisation for UAV-assisted networks in the presence of eavesdroppers: a multi-armed bandit approach
Authors: Flores Cabezas Xavier Alejandro , Moya Osorio Diana Pamela , Latva-aho Matti 
Published: September 2022

AbstractUnmanned aerial vehicles (UAVs) are becoming increasingly attractive for the ambitious expectations for 5G and beyond networks due to their several benefits. Indeed, UAV-assisted communications introduce a new range of challenges and opportunities regarding the security of these networks. Thus, in this paper we explore the opportunities that UAVs can provide for physical layer security solutions. Particularly, we analyse the secrecy performance of a ground wireless communication network assisted by N friendly UAV jammers in the presence of an eavesdropper. To tackle the secrecy performance of this system, we introduce a new area-based metric, the weighted secrecy coverage (WSC), that measures the improvement on the secrecy performance of a system over a certain physical area given by the introduction of friendly jamming. Herein, the optimal 3D positioning of the UAVs and the power allocation is addressed in order to maximise the WSC. For that purpose, we provide a reinforcement learning-based solution by modelling the positioning problem as a multi-armed bandit problem over three positioning variables for the UAVs: angle, height and orbit radius. Our results show that the proposed algorithm improves the secrecy of the system over time in terms of the WSC, and it converges into a stable state close to the exhaustive search solution for discretised actions, where there is a trade-off between expediency of the positioning of the UAVs to positions of better secrecy outcome and energy consumption.
Direct and indirect effects of IFN-α2b in malignancy treatment: not only an archer but also an arrow
Authors: Xiong Fei , Wang Qi , Wu Guan-hua , Liu Wen-zheng , Wang Bing , Chen Yong-jun 
Published: September 2022

AbstractInterferon-α2b (IFN-α2b) is a highly active cytokine that belongs to the interferon-α (IFN-α) family. IFN-α2b has beneficial antiviral, antitumour, antiparasitic and immunomodulatory activities. Direct and indirect antiproliferative effects of IFN-α2b have been found to occur via multiple pathways, mainly the JAK-STAT pathway, in certain cancers. This article reviews mechanistic studies and clinical trials on IFN-α2b. Potential regulators of the function of IFN-α2b were also reviewed, which could be utilized to relieve the poor response to IFN-α2b. IFN-α2b can function not only by enhancing the systematic immune response but also by directly killing tumour cells. Different parts of JAK-STAT pathway activated by IFN-α2b, such as interferon alpha and beta receptors (IFNARs), Janus kinases (JAKs) and IFN‐stimulated gene factor 3 (ISGF3), might serve as potential target for enhancing the pharmacological action of IFN-α2b. Despite some issues that remain to be solved, based on current evidence, IFN-α2b can inhibit disease progression and improve the survival of patients with certain types of malignant tumours. More efforts should be made to address potential adverse effects and complications.
Individually moulded silicone dressing in full thickness skin grafts
Authors: Götting Michael , Zibell Rahel , Jungehülsing Markus 
Published: September 2022

Background Full thickness skin grafting is a common technique for reconstructing defects in the head and neck area. We propose the use of an addition-cured silicone as an individually moulded silicone dressing to keep the vulnerable skin graft in place, prevent shearing forces and create a moist environment. Method The silicone dressing is applied directly on the graft, using a double cartridge system. The silicone is moulded to the skin graft and hardens quickly, integrating thread knots into its material and creating good adherence to the graft. Charts of 24 patients who had undergone reconstruction with full thickness skin graft from the neck after surgery for skin tumors in the head from November 2017 to October 2020, were reviewed retrospectively to quantify the degree of post-operative graft loss and durability of the dressing. Conclusion Medical silicone based on venylpolysiloxane is a safe and durable dressing which makes postoperative dressing changes expendable. Trial registration The study was approved by the institutional review board of the Brandenburg state medical association (S 4(bB)/2021). Graphical Abstract
UV protection effect of cotton dyed with Flos Sophorae (Sophora Japonica L.) extracted with acid hydrolysis
Authors: Narantuya Lkhagva , Ahn Cheunsoon 
Published: September 2022

AbstractFlos Sophorae extract was hydrolyzed using a mixed solution of methanol, HCl, and water to convert rutin to quercetin for the purpose of enhancing the UV protective effect of dyed cotton. Relative yield (%) of quercetin in the hydrolyzed extract was determined by the percent ratio of the absorbance of untreated Flos Sophorae extract at 359 nm to the absorbance of hydrolyzed extract at 370–371 nm. Quercetin yield was 93.09% when 1 M HCl was applied and 52.03% when 0.5 M HCl was applied, both with 60 min reaction time. The latter was applied in dyeing cotton with the dyebath containing hydrolyzed Flos Sophorae extract and water by 50:50 or 75:25 ratio. By dyeing with hydrolyzed Flos Sophorae extract the UPF value of cotton was enhanced to 13.80 indicating 92.76% blockage of the UV rays as opposed to 84.78% blockage by the same fabric without Flos Sophorae dyeing. The present method could produce a light-weight summer cotton with yellow color which could provide a moderate UV protection for daily summer attire.
A rare case of eosinophilic gastrointestinal disorders with short bowel syndrome after strangulated bowel obstruction
Authors: Arai Yuhki , Kinoshita Yoshiaki , Kobayashi Takashi , Takahashi Yoshiaki , Ohyama Toshiyuki , Yokota Naoki , Sugai Yu , Takano Shoichi , Hamasaki Yu , Kaneko Utako , Kanada Satoshi 
Published: September 2022

Abstract
Background
Short bowel syndrome (SBS) is a rare yet costly disease with an incidence rate of 3 per million people. Herein, we report a rare case of eosinophilic gastrointestinal disorders (EGIDs) with SBS after strangulated bowel obstruction.

Case presentation
A 5-year-old male had a necrotic intestine of 340 cm resected due to strangulated bowel obstruction caused by an intestinal mesenteric hiatal hernia. The length of the residual intestine was 51 cm. Bloody stools appeared 19 days postoperatively. Colonoscopy showed diffuse redness of the colonic mucosa, and pathological findings showed moderate chronic inflammatory cellular infiltration. On blood examination, the eosinophil count was > 30%. EGIDs with short bowel syndrome (SBS) were suspected. Because his symptoms did not improve with initial nutrition therapy, he was transferred to our hospital 5 months after the operation. Prednisolone was administrated at an initial dose of 1.4 mg/kg/day, 6 days after his transfer. Bloody stools disappeared after prednisolone administration. Seven months after discharge, he had no bloody stool recurrence.

Conclusion
The risk of developing secondary EGIDs in children with SBS should be considered, and postoperative management should include attention to abdominal symptoms and elevated eosinophil counts on blood examination.
Translational design for limited resource settings as demonstrated by Vent-Lock, a 3D-printed ventilator multiplexer
Authors: Xun Helen , Shallal Christopher , Unger Justin , Tao Runhan , Torres Alberto , Vladimirov Michael , Frye Jenna , Singhala Mohit , Horne Brockett , Kim Bo Soo , Burke Broc , Montana Michael , Talcott Michael , Winters Bradford , Frisella Margaret , Kushner Bradley S. , Sacks Justin M. , Guest James K. , Kang Sung Hoon , Caffrey Julie 
Published: September 2022

Abstract
Background
Mechanical ventilators are essential to patients who become critically ill with acute respiratory distress syndrome (ARDS), and shortages have been reported due to the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

Methods
We utilized 3D printing (3DP) technology to rapidly prototype and test critical components for a novel ventilator multiplexer system, Vent-Lock, to split one ventilator or anesthesia gas machine between two patients. FloRest, a novel 3DP flow restrictor, provides clinicians control of tidal volumes and positive end expiratory pressure (PEEP), using the 3DP manometer adaptor to monitor pressures. We tested the ventilator splitter circuit in simulation centers between artificial lungs and used an anesthesia gas machine to successfully ventilate two swine.

Results
As one of the first studies to demonstrate splitting one anesthesia gas machine between two swine, we present proof-of-concept of a de novo, closed, multiplexing system, with flow restriction for potential individualized patient therapy.

Conclusions
While possible, due to the complexity, need for experienced operators, and associated risks, ventilator multiplexing should only be reserved for urgent situations with no other alternatives. Our report underscores the initial design and engineering considerations required for rapid medical device prototyping via 3D printing in limited resource environments, including considerations for design, material selection, production, and distribution. We note that optimization of engineering may minimize 3D printing production risks but may not address the inherent risks of the device or change its indications. Thus, our case report provides insights to inform future rapid prototyping of medical devices.
Quantitative Anatomic Comparison of the Extreme Lateral Transodontoid vs Extreme Medial Endoscopic Endonasal Approaches to the Jugular Foramen and Craniovertebral Junction
Authors: Abramov Irakliy , Labib Mohamed A. , Houlihan Lena Mary , Loymak Thanapong , Srinivasan Visish M. , Preul Mark C. , Lawton Michael T. 
Published: September 2022

BACKGROUND:
Large, destructive intracranial and extracranial lesions at the jugular foramen (JF) and anterior craniovertebral junction (CVJ) are among the most challenging lesions to resect.

OBJECTIVE:
To compare the extreme lateral transodontoid approach (ELTOA) with the extreme medial endoscopic endonasal approach (EMEEA) to determine the most effective surgical approach to the JF and CVJ.

METHODS:
Seven formalin-fixed cadaveric heads were dissected. Using neuronavigation, we quantitatively measured and compared the exposure of the intracranial and extracranial neurovascular structures, the drilled area of the clivus and the C1 vertebra, and the area of exposure of the brainstem.

RESULTS:
The mean total drilled area of the clivus was greater with the EMEEA than with the ELTOA (1043.5 vs 909.4 mm2, P = .02). The EMEEA provided a longer exposure of the extracranial cranial nerves (CNs) IX, X, and XI compared with the ELTOA (cranial nerve [CN] IX: 18.8 vs 12.0 mm, P = .01; CN X: 19.2 vs 10.4 mm, P = .003; and CN XI, 18.1 vs 11.9 mm, P = .04). The EMEEA, compared with the ELTOA, provided a significantly greater area of exposure of the contralateral ventromedial medulla (289.5 vs 80.9 mm2, P < .001) and pons (237.5 vs 86.2 mm2, P = .005) but less area of exposure of the ipsilateral dorsolateral medulla (51.5 vs 205.8 mm2, P = .008).

CONCLUSION:
The EMEEA and ELTOA provide optimal exposures to different aspects of the CVJ and JF. A combination of these approaches can compensate for their disadvantages and achieve significant exposure.
Observational study: determinants for vegetable consumption among elementary school students in Indonesia
Authors: Sofianita Nur Intania , Khomsan Ali , Setiawan Budi , Ekayanti Ikeu , Crosita Octaria Yessi 
Published: September 2022

Background: The prevalence of obesity in school children has been increasing worldwide, including in developing countries such as Indonesia. Childhood obesity is associated with an unbalanced diet, especially the lack of fruit and vegetable consumption. This study aimed to determine the factors that influence vegetable consumption behavior in school children in Indonesia. Methods: The cross-sectional study was conducted in eight public elementary schools in a suburban area of Bogor Regency involving 433 students from the fifth grade. Data collection was conducted from November to December 2018. Data on the students’ characteristics, knowledge, and attitude as well as availability of vegetables at home and at school were collected using a structured questionnaire. While, a 24-hour food recall was utilized to obtain information on vegetable consumption and overall diet. Data analysis was done using Chi-squared test and logistic regression. Results: The average daily intake of vegetables was 47.11 g, far below the recommend amount (250 g). Female students had significantly better vegetables intake compared with their male counterparts. The availability of vegetables at home and students’ positive attitude toward vegetables were also significantly associated with better vegetable intake. Logistic regression analysis showed that availability of vegetables at home almost tripled the likelihood of better vegetable consumption (OR 2.822, 95% CI 1.822–4.372). Additionally, availability of vegetables at school and positive attitude toward vegetables doubled the odd for better vegetable consumption, OR 2.2 and 2.1 respectively (p<0.005). Conclusions: Vegetable consumption among schoolchildren was very low compared with the recommended portion. Better vegetable consumption was significantly associated with sex, availability, and students’ attitudes. Vegetable availability at home was the strongest determinant influencing vegetable consumption among elementary school students involved in the study. Hence, parental support or home food environment is key in improving their diet quality to prevent obesity.
No association between fertility desire and HIV infections among men and women: Findings from community-based studies before and after implementation of an early antiretroviral therapy (ART) initiation program in the rural district of North-western Tanzania
Authors: Mkwashapi Denna , Todd Jim , Mahande Michael , Changalucha John , Urassa Mark , Marston Milly , Renju Jenny 
Published: September 2022

Background: Fertility is associated with the desire to have children. The impacts of HIV and antiretroviral therapy (ART) on fertility are well known, but their impacts on the desire for children are less well known in Tanzania. We used data from two studies carried out at different periods of ART coverage in rural Tanzania to explore the relationship between HIV infection and fertility desires in men and women. Methods: We conducted secondary data analysis of the two community-based studies conducted in 2012 and 2017 in the Magu Health and Demographic system site, in Tanzania. Information on fertility desires, HIV status, and social–economic and demographic variables were analyzed. Fertility desire was defined as whether or not the participant wanted to bear a child in the next two years. The main analysis used log-binomial regression to assess the association between fertility desire and HIV infection. Results: In the 2012 study, 43% (95% CI 40.7-45.3) of men and 33.3% (95% CI 31.8 - 35.0) women wanted another child in the next two years. In 2017 the percentage rose to 55.7% (95% CI 53.6 - 57.8) in men and 41.5% (95% CI 39.8 - 43.1) in women. Although fertility desire in men and women were higher in HIV uninfected compared to HIV infected, age-adjusted analysis did not show a statistical significance difference in both studies (2012: PR=1.02, 95%CI 0.835 - 1.174, p<0.915 and 2017: PR = 0.90 95%CI 0.743 - 1.084 p= 0.262). Discussion: One-third of women and forty percent of men desired for fertility in 2012, while forty percent of women and nearly half of men desired for fertility in 2017. The data showed fertility desire, in 2012 and 2017 were not related to HIV infection in both periods of ART coverage.