bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo reprogramming leads to premature death due to hepatic and intestinal failure
Authors: Parras Alberto , Vílchez-Acosta Alba , Desdín-Micó Gabriela , Mrabti Calida , Rechsteiner Cheyenne , Battiston Fabrice , Branchina Clémence , Pérez Kevin , Sempoux Christine , Ocampo Alejandro 
Published: May 2022

SUMMARYThe induction of cellular reprogramming by forced expression of the transcription factors OCT4, SOX2, KLF4, and C-MYC (OSKM) has been shown to allow the dedifferentiation of somatic cells and ameliorate age-associated phenotypes in multiple tissues and organs. Yet to date, the benefits of in vivo reprogramming are limited by the occurrence of detrimental side-effects. Here, using complementary genetic approaches, we demonstrated that continuous in vivo induction of the reprogramming factors leads to hepatic and intestinal dysfunction resulting in decreased body weight and premature death. By generating a novel transgenic reprogrammable mouse strain, which avoids OSKM expression in both liver and intestine, we drastically reduced the early lethality and adverse effects associated with in vivo reprogramming. This new reprogramming mouse allows safe and long-term continuous induction of OSKM and might enable a better understanding of in vivo reprogramming as well as maximize its potential effects on rejuvenation and regeneration.
Smartphone-Operated Affordable PCR Thermal Cycler for the Detection of Antimicrobial Resistant Bacterial Genes
Authors: Pudasaini Sanam , Thapa Garima , Marasini Bishnu P. , Giri Basant 
Published: May 2022

AbstractObjectivesAntimicrobial resistance (AMR) is a global public health threat. Surveillance of AMR requires affordable, rapid, and user-friendly diagnostic method. Our aim was to develop a low-cost thermocycler to perform polymerase chain reaction (PCR).MethodsWe developed a smartphone-operated PCR thermal cycler using locally available recycled materials. The thermal cycler was used for the amplification for three bacterial genes - bla-TEM and bla-CTXM and 16s rRNA in human urine samples. The performance of custom-built thermal cycler was compared with the commercial one.ResultsThe thermal cycler was portable (<1kg weight), required 12 V power supply, 25 µL of solution, and cost only USD50.0. Temperature and time conditions were instructed using a custom-built smartphone application. The ramping rate of was 0.23°C for heating and 0.43°C for cooling, set temperatures were within ± 0.5 °C of target showing a good thermal stability. The antibiotic sensitivity test of human urine samples showed they were highly resistance and multi-resistant. Nearly 46 % (n=54) E. coli isolates were positive in ESBL screening test. The custom-built thermocycler was able to accurately predict the presence of bla-TEM and bla-CTXM genes (n=6).ConclusionsWe developed and demonstrated a portable, low-cost, easy-to-use, and smartphone-operated PCR thermal cycler. Since it is portable, it can be used in remote location and field settings, including places without stable power supply. The use of the thermal cycler system can be extended, beyond the detection of AMR genes, e.g., in clinical diagnosis, genetics, forensic analysis, and environmental protection.
Zur and Zinc Increase Expression ofE. coliRibosomal Protein L31 Through RNA-Mediated Repression of the Repressor L31p
Authors: Rasmussen Rebecca A. , Camarillo Jeannie M. , Sosnowski Victoria , Cho Byoung-Kyu , Goo Young Ah , Lucks Julius B. , O’Halloran Thomas V. 
Published: May 2022

AbstractBacteria can adapt in response to numerous stress conditions. One such stress condition is zinc depletion. The zinc-sensing transcription factor Zur regulates the way enteric bacteria respond to severe changes in zinc availability. Under zinc sufficient conditions, Zn-loaded Zur (Zn2-Zur) is well-known to repress transcription of genes encoding zinc uptake transporters and paralogues of a few ribosomal subunits. Here, we report the discovery and mechanistic basis for the ability of Zur to up-regulate expression of the ribosomal protein L31 in response to zinc inE. coli. Through genetic mutations and reporter gene assays, we find that Zur achieves the up-regulation of L31 through a double repression cascade by which Zur first represses the transcription of L31p, a zinc-lacking paralogue of L31, which in turn represses the translation of L31. Mutational analyses show that translational repression by L31p requires an RNA hairpin structure within thel31mRNA and involves the N-terminus of the L31p protein. This work uncovers a new genetic network that allows bacteria to respond to host-induced nutrient limiting conditions through a sophisticated ribosomal protein switching mechanism.Graphical Abstract
Polyamines shift expression of macrophage L-arginine metabolism related-genes during Leishmania amazonensis infection
Authors: Zanatta Jonathan Miguel , Acuña Stephanie Maia , Bento Camilla de Almeida , Stolf Beatriz Simonsen , Muxel Sandra Marcia 
Published: May 2022

AbstractPolyamines are molecules involved in macrophage activation and polarization in response to pathogens. The interaction with Leishmania promotes modulation of macrophage immune response to favor arginine deviation to polyamines production, leading to parasite survival. Polyamine levels are a potential modulator of growth factors, driving cells to proliferation, wound healing, oxidative stress, or regulating translation. Here, we investigate the impact of L-arginine and polyamines in the transcriptional regulation of genes involved in arginine metabolization and pro-inflammatory response to L. amazonensis in murine BALB/c macrophages. We found that supplementation with L-arginine is insufficient to modulate macrophage gene expression and infection. Polyamine supplementation altered nitric oxide synthase (Nos2) and nitric oxide (NO) production, as well as other macrophage enzymes. Putrescine supplementation increased transcript levels of polyamine metabolism-related genes Arginase 2 (Arg2), Spermidine synthase (SpdS), and Spermine synthase (SpmS) in both uninfected and L. amazonensis infected macrophages. Putrescine increased Nos2 expression without leading to NO production, while L-arginine plus spermine led to production of NO in uninfected macrophages. Besides, L-arginine supplementation reduced the levels of Il-1b during infection, and L-arginine or L-arginine plus putrescine increased Mcp1 at 24h of infection. The percentage of infected macrophages was lower after putrescine, spermidine, and spermine supplementation than L-arginine supplementation. Our data showed that the polyamines shift L-arginine-metabolism related-genes on BALB/c macrophages and affect infection by L. amazonensis.
A small DNA virus initiates replication with no more than three genome copies per cell
Authors: Ren Ruifan , Zheng Limin , Han Junping , Carvalho Camila Perdoncini , Miyashita Shuhei , Zhang Deyong , Qu Feng 
Published: May 2022

AbstractCellular organisms purge lethal mutations as they occur (in haploids), or as soon as they become homozygous (in sexually reproducing diploids), thus making the mutation-carrying genomes the sole victims of lethality. How lethal mutations in viruses are purged remains an unresolved question because numerous viral genomes could potentially replicate in the same cell, sharing their encoded proteins, hence shielding lethal mutations from selection. Previous investigations by us and others suggest that viruses with plus strand (+) RNA genomes may compel such selection by bottlenecking the replicating genome copies in each cell to low single digits. However, it is unclear if similar bottlenecks also occur in cells invaded by DNA viruses. Here we investigated whether tomato yellow leaf curl virus (TYLCV), a small virus with a single-stranded DNA genome, underwent population bottlenecking in cells of its host plants. We engineered the TYLCV genome to produce two replicons that express green fluorescent protein and mCherry, respectively, in a replication-dependent manner. We found that less than 65% of cells penetrated by both replicons replicated both, whereas at least 35% of cells replicated either of them alone, illustrating an intracellular population bottleneck size of no more than three. Furthermore, sequential inoculations unveiled strong mutual exclusions of these two replicons in most cells. Collectively our data demonstrated for the first time that DNA viruses like TYLCV are subject to stringent intracellular population bottlenecks, suggesting that such population bottlenecks may be a virus-encoded, evolutionarily conserved trait that assures timely elimination of lethal mutations.Significance statementAn important unresolved issue in virus life cycles is how natural selection acts on individual virus copies in the same cells. Unlike cellular organisms in which genome copies with lethal or advantageous mutations usually share their hosts with no more than one homologous genome copy, viruses could potentially reproduce with numerous sister genomes per cell, permitting sharing of protein products, thereby greatly diminishing phenotypic impacts of otherwise eventful mutations. Previous investigations suggest that (+) RNA viruses solve this problem by bottlenecking the number of replicating genome copies to low single digits. The current study reveals strikingly similar intracellular population bottlenecks for a DNA virus. Further mechanistic interrogations could avail the virus-encoded bottleneck-enforcing apparatus as targets for antiviral therapy and prevention.
Effects of Cr and Ta additions on microstructure, corrosion-wear and electrochemical performances of laser cladded Fe90-Al2O3 coating in 3.5% NaCl solution
Authors: Yan Hu , Dejun Kong 
Published: May 2022

Purpose
This paper aims to investigate the effects of Cr and Ta additions on the friction performance and corrosion-wear mechanism of Fe90-Al2O3 coating in 3.5% NaCl solution.

Design/methodology/approach
Cr and Ta reinforced Fe90-Al2O3 coatings were prepared on Q235 steel by laser cladding. The effects of Cr and Ta addition on the coefficient of friction (COF) and wear rate of Fe90-Al2O3 coating were investigated using a friction tester, and the wear model was established to discuss its corrosion-wear mechanism.

Findings
The average COFs of Fe90-Al2O3, Fe90-Al2O3-10%Cr and Fe90-Al2O3-10%Ta coatings in 3.5% NaCl solution are 0.57, 0.42 and 0.75, respectively, and the corresponding wear rates are 9.42 × 10−7, 5.31 × 10−7 and 7.02 × 10−7 mm3 s−1 N−1, respectively. The corrosion-wear resistance of Fe90-Al2O3-10%Cr coating is the best among the three kinds of coatings, in which the additions of Cr and Ta play a role in solid solution strengthening.

Originality/value
The Fe90-Al2O3 coating was strengthened by the additions of Cr and Ta to improve its corrosion-wear resistance in 3.5% NaCl solution.
Randomized tax deadlines can help economy
Authors: Urenda Julio , Kosheleva Olga 
Published: May 2022

PurposeWhile the main purpose of reporting – e.g. reporting for taxes – is to gauge the economic state of a company, the fact that reporting is done at pre-determined dates distorts the reporting results. For example, to create a larger impression of their productivity, companies fire temporary workers before the reporting date and re-hire then right away. The purpose of this study is to decide how to avoid such distortion.Design/methodology/approachThis study aims to come up with a solution which is applicable for all possible reasonable optimality criteria. Thus, a general formalism for describing and analyzing all such criteria is used.FindingsThis study shows that most distortion problems will disappear if the fixed pre-determined reporting dates are replaced with individualized random reporting dates. This study also shows that for all reasonable optimality criteria, the optimal way to assign reporting dates is to do it uniformly.Research limitations/implicationsThis study shows that for all reasonable optimality criteria, the optimal way to assign reporting dates is to do it uniformly.Practical implications It is found that the individualized random tax reporting dates would be beneficial for economy.Social implications It is found that the individualized random tax reporting dates would be beneficial for society as a whole.Originality/valueThis study proposes a new idea of replacing the fixed pre-determining reporting dates with randomized ones. On the informal level, this idea may have been proposed earlier, but what is completely new is our analysis of which randomization of reporting dates is the best for economy: it turns out that under all reasonable optimality criteria, uniform randomization works the best.
Content posted by Spanish automotive sector brands on YouTube: branded content, insights and environmental commitment
Authors: Castelló-Martínez Araceli 
Published: May 2022

PurposeThe main objective of this study is to analyze content posted by automotive sector brands on their YouTube channels, identifying the use of a branded content format, the application of insights to creative conceptualization and the dissemination of messages related to messages' environmental commitment.Design/methodology/approachA descriptive study was conducted using qualitative content analysis as the methodological approach. The sample is made up of 691 videos posted on YouTube from September 2020 to August 2021 by the ten automotive brands with the biggest advertising budgets in Spain (InfoAdex, 2021).FindingsThe findings reveal a predominance of the advertorial format in content posted on YouTube by automotive sector brands, while the branded content format is less popular. Creative concepts based on insights are rare and limited to videos with an advertisement format. The theme of respect for the environment is used mainly in connection with the discourse on vehicle features and not so much as a commitment by the brand in response to consumer concerns or the changes that the automotive sector is undergoing.Originality/valueThe originality of the study lies in the approach it takes to content posted by automotive brands on YouTube. The paper's contribution is innovative in that the study monitors posts by brands in the sample on YouTube over the course of a year, rather than just during the specific periods when these brands run advertising campaigns.
Organising methods enabling integration for value creation in complex projects
Authors: Tampio Kari-Pekka , Haapasalo Harri 
Published: May 2022

Purpose
The purpose of this paper is to identify the areas and logic of integration of different stakeholders using different methods and to analyse their applicability and challenges in practical projects. The main aim is to describe how these different methods impact value creation.

Design/methodology/approach
Action design research was carried out in a large hospital construction project where the first author acted as an “involved researcher” and the second author acted as an “outside researcher”. Two workshops were organised to evaluate the direct and indirect challenges and benefits of the applied four methods and to explain how different methods enable value creation.

Findings
All the studied methods provide good results in terms of usability and commitment to the aims of the project, thus delivering the direct benefits expected. Process, people and tools logic works well in this case project when applying the methods properly. Significant evidence was provided on secondary deliverables of the methods, and all analysed methods had a significant impact in the area of leading people, clarifying what “focus on people” means and how it is enabled.

Practical implications
Focus on people can be achieved through different operative methods if applied in the right way. It is necessary to select the most suitable methods based on all the direct and indirect deliverables.

Originality/value
This case project offered a platform to analyse integration methods in a real-life project using the collaborative contract method. The authors were able to participate in the analysis by taking action from the very beginning of the project in terms of training, learning, continuous development and coaching of these methods and evaluating the applicability.
Does global value chain position affect innovation performance of China’s high-tech industries?
Authors: Yang Nana , Liu Qiming , Qian Furong , Wang Xinglong 
Published: May 2022

Purpose
Because of the rapid progress of global value chains (GVCs), it is worthwhile to study their impact on innovation. This study aims to explore the impact of GVC position of high-tech industries in the developing-country context of China on innovation performance; it also aims to explore the moderating effects of industrial agglomeration (specialization agglomeration and diversification agglomeration) on the relationship between GVC position and innovation performance.

Design/methodology/approach
The study is based on data gathered on Chinese high-tech industries in 30 provinces from the 2005–2015 period. The econometric analysis relies on merged data from the China Premium Database and the Trade in Value Added 2018 Database.

Findings
The regression results show that GVC position of China’s high-tech industries significantly affects their innovation performance, and both specialization agglomeration and diversification agglomeration significantly enhance the positive relationship between GVC position and innovation performance of China’s high-tech industries. After dividing the country into coastal and inland regions, new findings appear.

Originality/value
This study highlights the importance of GVC position and its effect on innovation performance of China’s high-tech industries. It contributes to the literature on the relationship between GVCs and innovation by elaborating on the moderating effects of industrial agglomeration on this relationship.
Effects of rTMS on working memory abilities and time-varying spectrum coherence of LFPS and spikes in rats
Authors: Guo Miao Miao , Wang Tian , Zhai Hao Di 
Published: May 2022

Purpose
The purpose of this study was to evaluate the effects of repetitive transcranial magnetic stimulation (rTMS) at different frequencies on working memory (WM) and neuroelectric activity in rats.

Design/methodology/approach
Three rTMS protocols involving different frequencies were applied to rats, and 16-channel local field potentials (LFPs) and spikes were recorded from the prefrontal cortex (PFC) of rats in each group during the WM task. First, the behavior of rats during the T-maze task was analyzed, and then, the firing rate of spikes and the energy of the θ-band and γ-band in LFPs when rats performed the WM tasks were calculated. Finally, the spectral coherence between LFPs and spikes was analyzed by wavelet transform.

Findings
The results showed that rats in the stimulation groups needed fewer days than those in the control group to reach the task correction standard during the WM experiment (p < 0.05). High-frequency rTMS increases the firing rate of spikes and the degree of synchronization of LFPs-spikes in the θ-band and γ-band in the WM process.

Originality/value
This study showed that high-frequency rTMS can improve the spatial learning ability of rats, which might be due to the increased neuronal excitability of the PFC and the enhancement of co-coding between different modes of neural signals. This study is helpful for understanding the neuroregulatory mechanism of rTMS and will provide a reference for the selection of a suitable frequency for TMS treatment.
Effect of magnetic field on output performance of magnetic liquid vibration energy harvester
Authors: Yang Xiaorui , Chen Ying , Zhang Yan , Yang Wenrong 
Published: May 2022

Purpose
The purpose of this study is to develop a friction-type energy-harvesting device based on magnetic liquids (MLs). This MLs energy-harvesting device combines MLs with a triboelectric nanogenerator (TENG), reducing the friction thermal effect to improve the conversion efficiency and working frequency band.

Design/methodology/approach
First, the motion equation of the MLs is calculated using the Bernoulli and fluid continuity equations. Second, the sloshing process of an ML in a container is simulated using the finite element simulation method, and the magnetic field distribution of the permanent magnet in the MLs is calculated. Then, the output characteristic of the ML-TENG is deduced theoretically, and the influencing factors of the output voltage are analyzed. Finally, the output voltage of the MLs energy-harvesting device was tested experimentally, and the influence of the magnetic field on the output voltage was tested.

Findings
This study proposes a vibration energy harvesting device based on MLs. The output voltage of the device was obtained through simulation and experimental tests, and the effect of the magnetic field on the output voltage was obtained.

Originality/value
This study provides a method to convert vibration energy into electrical energy using the magnetic response and fluid characteristics of MLs, and the availability of the device is verified by simulation and experiment. This energy-harvesting device exhibits less loss and a more sensitive response.
Businesses and their community in times of COVID-19: a stakeholder theory approach
Authors: Ng David Wai Lun , Duarte Alonso Abel , Bressan Alessandro , Vu Oanh Thi Kim , Tran Thanh Duc , Atay Erhan 
Published: May 2022

PurposeThe purpose of this study is to build an understanding of how firms operate under the COVID-19 crisis. Specifically, the study examines the significance of firms’ survival for their surrounding community, business–community relations during this severe situation and factors helping firms address this unprecedented challenge. The conceptual tenets of stakeholder theory are considered.Design/methodology/approachA qualitative data collection approach was selected, with face-to-face and online interviews undertaken with owners and managers of 33 firms operating in Singapore.FindingsFirstly, while survival during the current unprecedented crisis is perceived to be most important for the firms’ employees, owners/managers also perceive domino effects on other companies working alongside the participating firms, as well as effects on clients and suppliers. Secondly, increased collaboration with industry, stronger partnerships with suppliers and business partners and closer relationships with staff are highlighted during the crisis. Thirdly, key factors helping firms’ survival in the initial year of the crisis included: a clear goal; a cohesive/nimble staff team; reinventing, adapting and being resilient; and family support and management support.Originality/valueThis study contributes empirically and conceptually to the literature on firm adaptation and firm–community relationships during a severe crisis. Empirically, the findings and related dimensions provide practical guidance concerning the interdependence between firms and their stakeholders. Conceptually, the inductive analysis, which enabled the development of a theoretical framework, illustrates the relationships between the study’s emerging dimensions and those predicated by stakeholder theory, namely, the descriptive, instrumental, normative and managerial.