bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coinfection of Clonorchis sinensis and hepatitis B virus: clinical liver indices and interaction in hepatic cell models
Authors: Dong Huimin , Zhao Lu , Sun Hengchang , Shang Mei , Lv Gang , Yu Xinbing , Hu Bo , Huang Yan 
Published: December 2022

Abstract
Background
In China, people infected with hepatitis B virus (HBV) are commonly found in areas with a high prevalence of Clonorchis sinensis, a trematode worm. Published studies have reported that the progression of hepatitis B is affected by coinfection C. sinensis.

Methods
Clinical data from a total of 72 patients with C. sinensis and HBV (as sole infection or with coinfections) and 29 healthy individuals were analysed. We also incubated the hepatic stellate cell line LX-2 with total proteins from C. sinensis adult worms (CsTPs) and HBV-positive sera. In addition, the human hepatoblastoma cell line HepG2.2.15 was treated with the antiviral drug entecavir (ETV), CsTPs and the anti-C. sinensis drug praziquantel (PZQ).

Results
Our clinical data indicated that the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB) and hyaluronic acid (HA) were significantly higher in patients with coinfection than in those infected with HBV only. In cell models, compared with the model in which LX-2 cells were incubated with HBV-positive sera (HBV group), transcripts of alpha-smooth muscle actin and types I and III collagen were significantly elevated in the models of LX-2 cells treated with CsTPs and HBV-positive sera (CsTP+HBV group), while the messenger RNA levels of tumour necrosis factor-α, interleukin (IL)-1β and IL-6 in the CsTP+HBV group were clearly lower. The HBV surface antigen and hepatitis B e-antigen levels were higher in the HepG2.2.15 cells treated with ETV and CsTPs than in those in the ETV group and in the cells administered a mixture of ETV, CsTPs and PZQ.

Conclusions
These results confirmed that C. sinensis and HBV coinfection could aggravate the progression of liver fibrosis. CsTPs might promote chronic inflammation of the liver in individuals with HBV infection, resulting in the development of hepatic fibrosis.

Graphic abstract
Molecular pathways, resistance mechanisms and targeted interventions in non-small-cell lung cancer
Authors: Wang Zixi , Xing Yurou , Li Bingjie , Li Xiaoyu , Liu Bin , Wang Yongsheng 
Published: December 2022

AbstractLung cancer is the leading cause of cancer-related mortality worldwide. The discovery of tyrosine kinase inhibitors effectively targeting EGFR mutations in lung cancer patients in 2004 represented the beginning of the precision medicine era for this refractory disease. This great progress benefits from the identification of driver gene mutations, and after that, conventional and new technologies such as NGS further illustrated part of the complex molecular pathways of NSCLC. More targetable driver gene mutation identification in NSCLC patients greatly promoted the development of targeted therapy and provided great help for patient outcomes including significantly improved survival time and quality of life. Herein, we review the literature and ongoing clinical trials of NSCLC targeted therapy to address the molecular pathways and targeted intervention progress in NSCLC. In addition, the mutations in EGFR gene, ALK rearrangements, and KRAS mutations in the main sections, and the less common molecular alterations in MET, HER2, BRAF, ROS1, RET, and NTRK are discussed. The main resistance mechanisms of each targeted oncogene are highlighted to demonstrate the current dilemma of targeted therapy in NSCLC. Moreover, we discuss potential therapies to overcome the challenges of drug resistance. In this review, we manage to display the current landscape of targetable therapeutic patterns in NSCLC in this era of precision medicine.
Study on mechanism and influential factors of progressive collapse resistance of base-isolated structure
Authors: Bao Chao , Zhang Yuhang , Lv Dahai , Wang Huxiang , Ma Xiaotong , Cao Jixing , Lim Kar Sing 
Published: December 2022

AbstractThe progressive collapse of the structure caused by the partial failure of the structure will cause severe consequences and massive losses, and structural progressive collapse resistance has always been a hot topic of current research. In order to study the progressive collapse mechanism of base-isolated structures, the test study and numerical simulation of the base-isolated structures were carried out based on the vertical Pushover method and analysis of the variation rule of the capacity of the remaining structure and influence mechanism. The isolation bearing failure position, the size of the beam of the seismic isolation layer, the type of the isolation bearing, and the horizontal stiffness of the seismic isolation layer on the capacity of the remaining structure were compared and analyzed. The results show that the non-uniformity of the beams and the concentrated loading at the nodes were easy to form a linear catenary mechanism, resulting in more severe beam end damage than mid-span damage. In the case of side isolation bearing failure, due to the lack of sufficient lateral restraint, the capacity was significantly lower than other conditions, which were more likely to cause partly collapse. Therefore, setting more transfer paths to improve the structure’s resistance to progressive collapses was necessary. Increasing the size of the beam of the seismic isolation layer could improve the capacity of the remaining structure of the alternate load path in the base-isolated structure. The changes in the horizontal stiffness of the seismic isolation layer and the type of the isolation bearing have little effect on the progressive collapse resistance capacity of the remaining structure.
Association of domain-specific physical activity with depressive symptoms: A population-based study
Authors: He Fan , Li Yi , Hu Zixin , Zhang Hui 
Published: December 2022

Abstract

Background
It remains unclear whether all physical activity (PA) domains (e.g., occupation-related PA [OPA], transportation-related PA [TPA], and leisure-time PA [LTPA]) have equivalent beneficial relationships. We aimed to investigate the associations of OPA, TPA, and LTPA with depressive symptoms in adults.

Methods
We included and analyzed 31,221 participants (aged ≥18 years) from the cross-sectional 2007–2018 U.S. National Health and Nutrition Examination Survey (NHANES). The PA domains were assessed by a self-report questionnaire and categorized based on the PA guidelines. Depressive symptoms were measured by the nine-item Patient Health Questionnaire.

Results
Participants achieving PA guidelines (≥150 min/week) were 26% (odds ratio [OR] 0.74, 95% confidence interval [CI] 0.68–0.80) and 43% (OR 0.57, 95% CI 0.51–0.62) less likely to have depressive symptoms depending on total PA and LTPA, respectively, while OPA or TPA did not demonstrate lower risks of depressive symptoms. LTPA at levels of 1–149, 150–299, and ≥300 min/week was associated with 31% (OR 0.69, 95% CI 0.60–0.78), 43% (OR 0.57, 95% CI 0.49–0.67), and 51% (OR 0.49, 95% CI 0.43–0.55) lower odds of depressive symptoms, respectively.

Conclusion
LTPA, but not OPA or TPA, was associated with a lower risk of depressive symptoms at any amount, suggesting that significant mental health would benefit from increased PA, even at levels below the recommendation.
Integrating Breast Cancer Early Detection Into a Resource-Constrained Primary Health Care System: Health Care Workers' Experiences in Rwanda
Authors: Uwimana Aimable , Dessalegn Sosina , Vianney Dusengimana Jean-Marie , Stauber Catherine , Fata Amanda , Hagenimana Marc , Uwinkindi Francois , Balinda Jean Paul , Shulman Lawrence N. , Revette Anna , Rwamuza Enock , Pace Lydia E. 
Published: December 2022

PURPOSE There is limited evidence to guide incorporation of breast cancer early detection into resource-constrained health systems where mammography screening is not yet available. To inform such strategies, we sought to understand health care workers' perspectives on a breast cancer early detection initiative integrated into community, primary, and secondary levels of care in Rwanda. METHODS We conducted a qualitative study using semistructured interviews with 33 community health workers, clinicians, and administrators at health facilities participating in the Women's Cancer Early Detection Program (WCEDP), through which women received clinical breast examination if they were receiving cervical cancer screening, or had breast concerns. Through thematic analysis, we identified dynamics and patterns associated with successes and challenges of the program's breast health services. RESULTS Successes and challenges identified by participants corresponded with the community- and primary care–based steps of cancer early diagnosis identified by the WHO. Regarding step 1 (community awareness/access), participants noted increases in awareness and care-seeking. Challenges included difficulty overcoming stigma and engaging older women. Regarding step 2 (clinical evaluation), all participants described increased breast health knowledge, skills, and confidence. Integrating the WCEDP with other services was challenging because of inadequate staffing; offering WCEDP services on a designated day/week had advantages and disadvantages. Although participants appreciated WCEDP referral mechanisms, they desired more communication from referral facilities. Patients' poverty was the most consistently identified impediment to referral completion. CONCLUSION Rwandan health care workers identified real-world successes and challenges of implementing principles of early cancer diagnosis for breast cancer early detection. Future interventions should focus on engagement of older women, community awareness, patient socioeconomic support, and optimizing integration into primary care.
Genome-Wide Association Study Identifies ROBO2 as a Novel Susceptibility Gene for Anthracycline-Related Cardiomyopathy in Childhood Cancer Survivors
Authors: Wang Xuexia , Singh Purnima , Zhou Liting , Sharafeldin Noha , Landier Wendy , Hageman Lindsey , Burridge Paul , Yasui Yutaka , Sapkota Yadav , Blanco Javier G. , Oeffinger Kevin C. , Hudson Melissa M. , Chow Eric J. , Armenian Saro H. , Neglia Joseph P. , Ritchey A. Kim , Hawkins Douglas S. , Ginsberg Jill P. , Robison Leslie L. , Armstrong Gregory T. , Bhatia Smita 
Published: December 2022

PURPOSE Interindividual variability in the dose-dependent association between anthracyclines and cardiomyopathy suggests a modifying role of genetic susceptibility. Few previous studies have examined gene-anthracycline interactions. We addressed this gap using the Childhood Cancer Survivor Study (discovery) and the Children's Oncology Group (COG) study COG-ALTE03N1 (replication). METHODS A genome-wide association study (Illumina HumanOmni5Exome Array) in 1,866 anthracycline-exposed Childhood Cancer Survivor Study participants (126 with heart failure) was used to identify single-nucleotide polymorphisms (SNPs) with either main or gene-environment interaction effect on anthracycline-related cardiomyopathy that surpassed a prespecified genome-wide threshold for statistical significance. We attempted replication in a matched case-control set of anthracycline-exposed childhood cancer survivors with (n = 105) and without (n = 160) cardiomyopathy from COG-ALTE03N1. RESULTS Two SNPs (rs17736312 [ ROBO2]) and rs113230990 (near a CCCTC-binding factor insulator [< 750 base pair]) passed the significance cutoff for gene-anthracycline dose interaction in discovery. SNP rs17736312 was successfully replicated. Compared with the GG/AG genotypes on rs17736312 and anthracyclines ≤ 250 mg/m2, the AA genotype and anthracyclines > 250 mg/m2 conferred a 2.2-fold (95% CI, 1.2 to 4.0) higher risk of heart failure in discovery and an 8.2-fold (95% CI, 2.0 to 34.4) higher risk in replication. ROBO2 encodes transmembrane Robo receptors that bind Slit ligands (SLIT). Slit-Robo signaling pathway promotes cardiac fibrosis by interfering with the transforming growth factor-β1/small mothers against decapentaplegic (Smad) pathway, resulting in disordered remodeling of the extracellular matrix and potentiating heart failure. We found significant gene-level associations with heart failure: main effect ( TGF-β1, P = .007); gene*anthracycline interaction ( ROBO2*anthracycline, P = .0003); and gene*gene*anthracycline interaction ( SLIT2* TGF-β1*anthracycline, P = .009). CONCLUSION These findings suggest that high-dose anthracyclines combined with genetic variants involved in the profibrotic Slit-Robo signaling pathway promote cardiac fibrosis via the transforming growth factor-β1/Smad pathway, providing credence to the biologic plausibility of the association between SNP rs17736312 ( ROBO2) and anthracycline-related cardiomyopathy.
Broadband ultrasonic focusing based on circular Airy-like beam
Authors: Zhu Xing-Feng , Wu Da-Jian , Yao Jie , Wei Qi , Liu Xiao-Jun 
Published: December 2022

Abstract
Convergence of ultrasonic energy is of great importance in the medical and engineering fields. In this work, ultrasonic focusing based on circular Airy-like beam (CALB) is numerically and experimentally demonstrated. The CALB focusing can be achieved simply using a planar resin ring lens immersed in water. At 2 MHz, the acoustic intensity at the focus can reach 257 times that for the incident wave. Arising from the self-healing feature of Airy beams, the CALB focusing can be well maintained even if there exists an obstacle in the acoustic path. Meanwhile, the focusing behaviour is well performed in a broad frequency range of 1.5 MHz to 3.1 MHz due to the simple and non-resonant design. By adjusting the frequency of the incident waves, the focal length can be modified without changing the lens structure. The CALB focusing and the corresponding planar lens may promote wide applications in ultrasound imaging, non-destructive testing, acoustic tweezing and medical diagnostic techniques.
Potential impedance reduction by REBCO-coated conductors as beam screen coating for the Future Circular Hadron Collider
Authors: Krkotić P. , Tagdulang N. D. , Calatroni S. , O'Callaghan J. M. , Pont M. 
Published: December 2022

Abstract
The Future Circular Collider study creates a conceptual design for a post-LHC particle accelerator using 16 T superconducting dipoles to achieve collision energies of up to 100 TeV in a 90 km circumference ring. A copper-coated beam screen, similar to that used in the LHC, is planned. However, the undertaken research indicates that copper at the high working temperature of 50 K has a strong influence on the accelerator's performance, particularly at injection energy. In this work, we relate the experimentally determined properties of REBCO-coated conductors with their potential performance in the FCC-hh beam screen. Specifically, we use a round pipe approximation to demonstrate that a beam screen coated with a combination of REBCO and copper can have a much lower resistive wall impedance than one using only copper. The reduction is substantial (several orders of magnitude), and is observed in both the longitudinal and transverse wall impedance. Such a reduction has important effects on beam stability, operating costs, potential reduction in beam screen size, and lowering the stringent specifications of the 16 T magnets required for the Future Circular Hadron Collider.
The “In-Parallel” Technique for Awake, Bilateral Simultaneous Minimally Invasive Transforaminal Lumbar Interbody Fusion and Multilevel Lumbar Decompression
Authors: Chan Andrew K. , Gnaedinger Anika , Ayoub Chakib , Gupta Dhanesh K. , Abd-El-Barr Muhammad M. 
Published: December 2022

BACKGROUND:
Minimally invasive transforaminal lumbar interbody fusion (MIS TLIF) and MIS lumbar decompression have been successfully undertaken in the absence of general anesthesia in well-selected patients. By leveraging spinal anesthesia, surgeons may safely conduct surgeries on one- or two-level lumbar pathology. However, surgeries on more extensive pathology have not yet been described, because of concerns about the duration of efficacy of spinal anesthetic in the awake patient.

OBJECTIVE:
To report the use of a novel awake technique for “in parallel,” simultaneous lumbar spinal surgery on three-segment pathology.

METHODS:
We describe concurrent performance of a dual microscopic, navigated MIS TLIF and MIS two-level decompression, using a combination of liposomal bupivacaine erector spinae block in conjunction with a spinal anesthetic.

RESULTS:
We show that a left-sided, two-level MIS tubular microscopic decompression combined with a concurrent right-sided, transfacet MIS TLIF via a tubular microscopic approach was well tolerated in an 87-year-old patient with multilevel lumbar stenosis with a mobile spondylolisthesis.

CONCLUSION:
We provide the first description of a dual-surgeon approach for minimally invasive spine surgery. This “in-parallel” technique—reliant on 2, independent spine surgeons—may aid in the performance of surgeries previously considered too extensive, given the time constraints of regional anesthesia and can be successfully applied to patients who otherwise may not be candidates for general anesthesia.
Forearm Flexor Tendon Injury in Adolescent Athletes: Risk Factors, Treatment, and Prevention
Authors: Elhage Kareem Giovanni , Yedulla Nikhil Reddy , Cross Austin George , Mehta Nabil , Guo Eric W. , Bernstein David N. , Makhni Eric 
Published: December 2022

Abstract
Injury to the flexor pronator mass is a common condition that is especially prevalent in overhead throwing athletes. The increasing incidence of these injuries has promoted considerable efforts in research to better understand the pathology, risk factors, and potential mechanisms to prevent injury in these athletes. While there are numerous intrinsic and extrinsic factors associated with injury, a common theme involves chronic overuse and microtrauma with inadequate resting intervals between performances. The purpose of this review is to discuss medial elbow injuries in young athletes with a particular focus on the flexor pronator mass.
Assessing Performing Artists in Medical and Health Practice — The Dancers, Instrumentalists, Vocalists, and Actors Screening Protocol
Authors: Ackermann Bronwen J. , Guptill Christine , Miller Clay , Dick Randall , McCrary J. Matt 
Published: December 2022

Abstract
Training in the performing arts exposes individuals to often extreme physical and psychological demands, which are linked to high occupational injury rates. The intense demands of performing artists have been likened to those of sport athletes. However, distinct differences in these demands necessitate specialized approaches to the health care of performing artists. Through the Athletes and the Arts collaboration, the American College of Sports Medicine and Performing Arts Medicine Association identified that the creation of a specialized preparticipation screening tool for performing artists would likely enhance health care for performing artists significantly. Based on a thorough review of established assessments and an extensive consultation process with domain experts, a consensus best-practice screening tool was developed: the Dancer, Instrumentalist, Vocalist, Actor (DIVA) Preparticipation Screening. This screening tool is modeled on the athletic preparticipation examination (PPE) in its structure and 30-min target duration. However, DIVA diverges considerably from the PPE in its content to address the specific risks and needs of performing artists. In particular, screening questions and physical examination procedures focus strongly on musculoskeletal injuries and mental health conditions, in response to the preponderance and interactions of these conditions appearing in performing artists. The DIVA tool presented is intended as a “living tool,” which can be modified in the future to include new effective assessment techniques as appropriate. Training in the DIVA preparticipation physical examination is included as a core component of the essentials of performing arts medicine continuing education course described in detail in a companion manuscript in this issue.
The conditional effect of family resilience on family quality of life during the Covid-19 pandemic
Authors: Setiawan Tery , Wardani Ria , Theresia Ellen 
Published: December 2022

Introduction: The Covid-19 pandemic has brought unprecedented challenges, both economically and psychologically, to most families across the world. Yet, little study has been done on this topic in Indonesia, even after the second year of the pandemic. This study examines how the Covid-19 economic impact and parental stress are related to the family quality of life (FQOL) and how the relation is moderated by family resilience. Methods: To this end, we employed previously tested measures to assess the level of parental stress, family resilience and FQOL. Especially for the latter, we modified the measure, developed by Beach Center on Disability, by including only four domains (i.e., family interaction, parenting, emotional well-being, and material well-being) to adjust to our research context. Results: Based on 169 participants, our CFA displays that all employed measures in the study are valid and reliable. Our regression analysis shows that there are significant direct relations of parental stress & family resilience with family quality of life. However, we find that family resilience only positively moderates the relation between the Covid-19 economic impact and family quality of life. Discussion: This study presents a view on how the Covid-19 pandemic affects the way families live and hence, their quality of life. In addition, the findings suggest the importance of family relationship and support in times of crisis, not limited to the Covid-19 pandemic.
Correlation of TcII discrete typing units with severe chronic Chagas cardiomyopathy in patients from various Brazilian geographic regions
Authors: Tavares de Oliveira Maykon , Fuzo Carlos Alessandro , da Silva Maria Cláudia , Donadi Eduardo Antônio , da Silva João Santana , Moreira Henrique Turin , Schmidt André , Marin-Neto José Antônio 
Published: December 2022

Background
Chagas disease (ChD) is caused by Trypanosoma cruzi. The genetic structure of the species is divided into seven distinct genetic groups, TcI to TcVI, and Tcbat, which have shown differences in terms of geographic distribution, biological properties, and susceptibility to drugs. However, the association between genetic variability and clinical forms of ChD has not yet been fully elucidated. The predominance of TcII and TcVI discrete typing units (DTUs) (genetic groups) is known to occur in several Brazilian regions and is associated with both the domestic and the wild cycles of ChD. Thus, this study aimed to verify the genotypes of the parasites present in 330 patients with chronic Chagas cardiomyopathy (CCC) from different Brazilian states attended at the Clinical Hospital of the Ribeirão Preto Medical School and to assess the existence of a correlation between the clinical forms with the main cardiovascular risk factors and the genetics of the parasite.

Methodology Principal findings
All patients with CCC were clinically evaluated through anamnesis, physical examination, biochemical tests, 12-lead electrocardiogram, echocardiogram and chest X-ray. Peripheral blood (5 mL) was collected in guanidine/ethylenediaminetetraacetic acid from each patient for DNA extraction and real-time polymerase chain reaction (PCR) for Chagas disease and genotyping of the parasite in the 7 DTUs. Parasite genotyping was performed using conventional multilocus PCR. Samples of only 175 patients were positive after amplification of the specific genes contained in the T. cruzi genotyping criteria. TcII (64/175), TcVI (9/175), and TcI (3/175) DTUs were predominant, followed by TcII/TcV/TcVI (74/175), and TcII/TcVI (23/175). The TcIII and TcIV DTU´s was detected in only one sample of CCC patients.

Conclusions/Significance
Our data corroborate previous findings, indicating the predominance of the TcII genotype in patients with CCC of Brazilian origin. Moreover, this study pioneered disclosing a direct correlation between the TcII DTU and severe CCC.