bluuebunny/crossref_metadata_2025_split huggingface dataset:
Down-regulated lncRNA ROR in tumor-educated platelets as a liquid-biopsy biomarker for nasopharyngeal carcinoma
Authors: Wei Jiazhou , Meng Xian , Wei Xiuqi , Zhu Kaidong , Du Li , Wang Hui 
Published: September 2022

Abstract
Purposes
To evaluate the diagnostic value of tumor-educated platelets (TEP) lncRNA ROR for nasopharyngeal carcinoma (NPC).

Methods
Quantitative real-time PCR was used to determine the expression level of TEP lncRNA ROR in NPC patients (n = 50) as compared to normal subjects (n = 33). The ROC curve analysis was performed to assess the diagnostic value of TEP lncRNA ROR for NPC. Correlations between TEP lncRNA ROR and clinical parameters were further analyzed.

Results
The median of TEP lncRNA ROR was significantly lower in NPC patients than that in normal subjects (0.0209 vs 0.0610, p = 0.0019), while no significant difference was found in plasma lncRNA ROR. ROC analysis showed that TEP lncRNA ROR had a sensitivity of 60%, specificity of 70%, and accuracy of 63.9% in diagnosing NPC, and the area under ROC curve (AUC) was 0.70. The expression level of TEP lncRNA ROR in NPC showed no significant difference among different TNM stages. However, low level of TEP lncRNA ROR correlated well with positive Epstein–Barr virus (EBV) DNA (kappa value = 0.314, p = 0.06), TEP lncRNA ROR and EBV DNA had similar diagnostic positive rate (58.3%) for NPC, and the combination of TEP lncRNA ROR and EBV DNA increased the positive rate to 74%.

Conclusion
The expression level of TEP lncRNA ROR was down-regulated in NPC and the diagnostic value of TEP lncRNA ROR was similar to EBV DNA. Our study indicated that TEP lncRNA ROR might serve as a novel type of liquid biopsy biomarker in diagnosis of NPC patients.
False positives: handling them operationally for automatic pollen monitoring
Authors: Crouzy Benoît , Lieberherr Gian , Tummon Fiona , Clot Bernard 
Published: September 2022

AbstractThis note provides a generic method for handling falsely identified particles (false positive detections) in automatic pollen monitoring systems. The benefits of the method are illustrated using data from two different automatic pollen monitoring devices (Swisens Poleno and Plair Rapid-E) in comparison to reference Hirst-type pollen measurements. Neither climatological nor phenological information are introduced to filter out false positive detections: the method is based only on the measurements.
Role of nanocellulose in tailoring electroanalytical performance of hybrid nanocellulose/multiwalled carbon nanotube electrodes
Authors: Durairaj Vasuki , Liljeström Touko , Wester Niklas , Engelhardt Peter , Sainio Sami , Wilson Benjamin P. , Li Panpan , Kontturi Katri S. , Tammelin Tekla , Laurila Tomi , Koskinen Jari 
Published: September 2022

AbstractNanocellulose has emerged as a promising green dispersant for carbon nanotubes (CNTs), and there is an increasing trend in developing nanocellulose/CNT hybrid materials for electrochemical detection of various small molecules. However, there have been very few comprehensive studies investigating the role of nanocellulosic material properties upon the electroanalytical performance of the resultant hybrid electrodes. In this work, we demonstrate the influence of both nanocellulose functionalization and geometry, utilizing sulfated cellulose nanocrystals, sulfated cellulose nanofibers, and TEMPO-oxidized cellulose nanofibers. Transmission electron microscopy tomography enables direct visualization of the effect of nanocellulosic materials on the hybrid architectures. High resolution X-ray absorption spectroscopy verifies that the chemical nature of CNTs in the different hybrids is unmodified. Electroanalytical performances of the different nanocellulose/CNT hybrid electrodes are critically evaluated using physiologically relevant biomolecules with different charge such as, dopamine (cationic), paracetamol (neutral), and uric acid (anionic). The hybrid electrode containing fibrillar nanocellulose geometry with a high degree of sulfate group functionalization provides the highest electroanalytical sensitivity and strongest enrichment towards all studied analytes. These results clearly demonstrate for the first time, the extent of tailorability upon the electroanalytical response of nanocellulose/CNT hybrid electrodes towards different biomolecules, offered simply by the choice of nanocellulosic materials.
Decentralized cross-blockchain asset transfers with transfer confirmation
Authors: Sober Michael , Sigwart Marten , Frauenthaler Philipp , Spanring Christof , Kobelt Max , Schulte Stefan 
Published: September 2022

AbstractToday, several solutions for cross-blockchain asset transfers exist. However, these solutions are either tailored to specific assets or neglect finality guarantees that prevent assets from getting lost in transit. In this paper, we present a cross-blockchain asset transfer protocol that supports arbitrary assets, is adaptable to different means of cross-blockchain communication, and adheres to requirements such as finality. The ability to freely transfer assets between blockchains may increase transaction throughput and provide developers with more flexibility by allowing them to design digital assets that leverage the capacities and capabilities of multiple blockchains. We define the general requirements and specifications for a cross-blockchain asset transfer protocol and provide a proof-of-concept implementation for EVM-based blockchains. Further, we evaluate the protocol concerning costs, transfer duration, and security.
Alert threshold assessment based on equivalent displacements for the identification of potentially critical landslide events
Authors: Valletta Alessandro , Carri Andrea , Segalini Andrea 
Published: September 2022

AbstractOver the past years, the growing number of natural hazards all over the world has led to an increasing focus on activities aimed at studying and controlling the occurrence of these phenomena. In this context, monitoring systems have become a fundamental component for Landslide Early Warning Systems, allowing to understand the evolution of these processes and assess the need for dedicated mitigation measures. This result is achieved thanks to several technological advancements that led to the introduction of more accurate and reliable sensors, as well as automatic procedures for data acquisition and elaboration. However, despite these improvements, the data interpretation process is still a challenging task, in particular when it comes to the identification of critical events and failure forecasting operations. This paper presents a methodology developed to assess if a potentially critical event is displaying a significant deviation from previously sampled data, or if it could be classified as a false alarm. The process relies on the definition of a threshold value based on the landslide behavior preceding the event of interest. In particular, the reference value derives from the evaluation of equivalent displacements, defined as the displacements previously observed in a time interval equal to the one showed by the potentially critical event. This paper reports a series of examples referring to different case studies, involving both false alarms and real collapses, underlining the effectiveness of the proposed model as a useful tool to evaluate the landslide behavior with a near-real-time approach.
Maximum interpolable gap length in missing smartphone-based GPS mobility data
Authors: McCool Danielle , Lugtig Peter , Schouten Barry 
Published: September 2022

AbstractPassively-generated location data have the potential to augment mobility and transportation research, as demonstrated by a decade of research. A common trait of these data is a high proportion of missingness. Naïve handling, including list-wise deletion of subjects or days, or linear interpolation across time gaps, has the potential to bias summary results. On the other hand, it is unfeasible to collect mobility data at frequencies high enough to reflect all possible movements. In this paper, we describe the relationship between the temporal and spatial aspects of these data gaps, and illustrate the impact on measures of interest in the field of mobility. We propose a method to deal with missing location data that combines a so-called top-down ratio segmentation method with simple linear interpolation. The linear interpolation imputes missing data. The segmentation method transforms the set of location points to a series of lines, called segments. The method is designed for relatively short gaps, but is evaluated also for longer gaps. We study the effect of our imputation method for the duration of missing data using a completely observed subset of observations from the 2018 Statistics Netherlands travel study. We find that long gaps demonstrate greater downward bias on travel distance, movement events and radius of gyration as compared to shorter but more frequent gaps. When the missingness is unrelated to travel behavior, total sparsity can reach levels of up to 20% with gap lengths of up to 10 min while maintaining a maximum 5% downward bias in the metrics of interest. Temporal aspects can increase these limits; sparsity occurring in the evening or night hours is less biasing due to fewer travel behaviors.
Episodic memory dysfunction and hypersynchrony in brain functional networks in cognitively intact subjects and MCI: a study of 379 individuals
Authors: Chino Brenda , Cuesta Pablo , Pacios Javier , de Frutos-Lucas Jaisalmer , Torres-Simón Lucía , Doval Sandra , Marcos Alberto , Bruña Ricardo , Maestú Fernando 
Published: September 2022

AbstractDelayed recall (DR) impairment is one of the most significant predictive factors in defining the progression to Alzheimer’s disease (AD). Changes in brain functional connectivity (FC) could accompany this decline in the DR performance even in a resting state condition from the preclinical stages to the diagnosis of AD itself, so the characterization of the relationship between the two phenomena has attracted increasing interest. Another aspect to contemplate is the potential moderator role of the APOE genotype in this association, considering the evidence about their implication for the disease. 379 subjects (118 mild cognitive impairment (MCI) and 261 cognitively intact (CI) individuals) underwent an extensive evaluation, including MEG recording. Applying cluster-based permutation test, we identified a cluster of differences in FC and studied which connections drove such an effect in DR. The moderation effect of APOE genotype between FC results and delayed recall was evaluated too. Higher FC in beta band in the right occipital region is associated with lower DR scores in both groups. A significant anteroposterior link emerged in the seed-based analysis with higher values in MCI. Moreover, APOE genotype appeared as a moderator between beta FC and DR performance only in the CI group. An increased beta FC in the anteroposterior brain region appears to be associated with lower memory performance in MCI. This finding could help discriminate the pattern of the progression of healthy aging to MCI and the relation between resting state and memory performance.
Accuracy of diagnostic modalities in suspected appendicitis in King Saud Hospital, Unaizah,Qassim, Saudi Arabia-retrospective cohort study
Authors: AlJohani Moath , AlSulaim Lamees , AlHarbi Abrar , AlSwuaileh Ahlam , AlHussain Noor , AlGhumayzi Anfal , AlMahlasi Afrah , Hathal alZamil  
Published: September 2022

Abstract

Objective
To retrospectively evaluate the accuracy of computed tomography (CT) ultrasonography (US) in the diagnosis of acute appendicitis, confirm the post-appendectomy result via histopathology, and compare the sensitivity and specificity of ultrasonography (US) to CT scans.

Methods
A retrospective cohort -chart review of 244 patient who underwent CT and US for diagnosis of suspected acute appendicitis in single center -King Saud Hospital, Unaizah, Qassim Region, Saudi Arabia was performed.

Result
All study participants underwent CT scans as part of their diagnostic workup. Of the 244 patients, 240 (98.4%) returned with findings suggestive of acute appendicitis. Of the total number of participants, 203 were confirmed via US out of which only 30.0% of them had features on US scans suggestive of acute appendicitis. The majority of the patients did not have any complications (83.6%)

Conclusion
CT scan can be considered the best modality to diagnose acute appendicitis and its complications with sensitivity of 98% and specifty of 16.7 compared to US, which shows 100% specificity and 29% sensitivity.

Highlights
No Blank Check
Authors: Reeves Andrew , Rogowski Jon C. 
Published: September 2022

Concerns about unaccountable executive power have featured recurrently in political debates from the American founding to today. For many, presidents' use of unilateral power threatens American democracy. No Blank Check advances a new perspective: Instead of finding Americans apathetic towards how presidents exercise power, it shows the public is deeply concerned with core democratic values. Drawing on data from original surveys, innovative experiments, historical polls, and contexts outside the United States, the book highlights Americans' skepticism towards presidential power. This skepticism results in a public that punishes unilaterally minded presidents and the policies they pursue. By departing from existing theories of presidential power which acknowledge only institutional constraints, this timely and revealing book demonstrates the public's capacity to tame the unilateral impulses of even the most ambitious presidents. Ultimately, when it comes to exercising power, the public does not hand the president a blank check.
A scoping review of community-based mental health intervention for children and adolescents in South Asia
Authors: Willmot Rhiannon A. , Sharp Rebecca A. , Amir Kassim Azlina , Parkinson John A. 
Published: September 2022

Abstract
Children and adolescents in South Asia are exposed to significant mental health risks. Yet, policy to prevent or treat youth mental health problems in this context is underdeveloped, and services are difficult to access. Community-based mental health treatment may offer a potential solution, by increasing resource capacity in deprived settings. However, little is known about the current community-based mental health provision for South Asian youth. A scoping review was conducted across six scientific databases and hand searching of reference lists to identify relevant studies. Study selection and data extraction were performed by three independent reviewers using predefined criteria, an adapted version of the template for intervention description and replication checklist and the Cochrane Risk of Bias Tool. The search identified 19 relevant studies published from January 2000 to March 2020. Studies most frequently addressed PTSD and autism, were conducted in India and Sri Lanka, used education-based intervention and were based in urban school settings. Community-based mental health provision for the South Asian youth is in its infancy, but holds promise for providing essential resources to treat or prevent mental health disorder. New insights on approaches are discussed, which are valuable for South Asian settings, primarily task-shifting and stigma reduction, with implications for policy, practice and research.
Anisakid parasites (Nematoda: Anisakidae) in 3 commercially important gadid fish species from the southern Barents Sea, with emphasis on key infection drivers and spatial distribution within the hosts
Authors: Levsen Arne , Cipriani Paolo , Palomba Marialetizia , Giulietti Lucilla , Storesund Julia E. , Bao Miguel 
Published: September 2022

AbstractNortheast Arctic cod, saithe and haddock are among the most important fisheries resources in Europe, largely shipped to various continental markets. The present study aimed to map the presence and distribution of larvae of parasitic nematodes in the Anisakidae family which are of socioeconomic and public health concern. Fishes were sourced from commercial catches during winter or spring in the southern Barents Sea. Samples of fish were inspected for nematodes using the UV-press method while anisakid species identification relied on sequencing of the mtDNA cox2 gene. Anisakis simplex (s.s.) was the most prevalent and abundant anisakid recorded, occurring at high infection levels in the viscera and flesh of cod and saithe, while being less abundant in haddock. Contracaecum osculatum (s.l.) larvae, not found in the fish flesh, showed moderate-to-high prevalence in saithe, haddock and cod, respectively. Most Pseudoterranova spp. larvae occurred at low-to-moderate prevalence, and low abundance, in the viscera (Pseudoterranova bulbosa) and flesh (Pseudoterranova decipiens (s.s.) and Pseudoterranova krabbei) of cod, only 2 P. decipiens (s.s.) appeared in the flesh of saithe. Body length was the single most important host-related factor to predict overall abundance of anisakid larvae in the fish species. The spatial distribution of Anisakis larvae in the fish flesh showed much higher abundances in the belly flaps than in the dorsal fillet parts. Trimming of the flesh by removing the belly flaps would reduce larval presence in the fillets of these gadid fish species by 86–91%.
Off‐Coast Phytoplankton Bloom in the Taiwan Strait During the Northeasterly Monsoon Wind Relaxation Period
Authors: Zhao Zhonghua , Oey Lie‐Yauw , Huang Bangqin , Lu Wenfang , Jiang Yuwu 
Published: September 2022

AbstractThis study applied cruise, model, and satellite data to analyze the off‐coast phytoplankton blooming during the late fall to early spring monsoon period in the Taiwan Strait when northeasterly wind prevails. Based on the composite and self‐organizing map analyses, the three data sets consistently show high chlorophyll‐a concentration near the along‐shore front during the down‐front northeasterly wind relaxation period while lower concentration when relatively strong wind is persistent. Meanwhile, the off‐coast blooming always coincides with intense near‐surface stratification when the northeasterly wind relaxes. Diagnoses of balanced Richardson number, Ertel potential vorticity and instability energy budget from high‐resolution cruise observations and model results demonstrate that vigorous submesoscale symmetric and baroclinic instabilities can develop near the along‐shore front under the down‐front NE wind. Diagnoses of modeled buoyancy and chlorophyll‐a budget equations further suggest the submesoscale instabilities lead to rapid near‐surface restratification and offshore stretching of the along‐shore front within the upper 10‐m of the mixed layer when the down‐front NE wind relaxes, favoring the surface 10‐m phytoplankton growth. As comparison, contribution of the larger‐scale advection related with geostrophic adjustment and Ekman transport to the chlorophyll‐a increment reached beyond the middle layer of ∼20‐m depth.
The effect of European fuel-tax cuts on the oil income of Russia
Authors: Gars Johan , Spiro Daniel , Wachtmeister Henrik 
Published: September 2022

AbstractFollowing Russia’s invasion of Ukraine, there has been a surge in transport fuel prices. Consequently, many European Union (EU) countries are cutting taxes on petrol and diesel to shield consumers. Using standard theory and empirical estimates, here we assess how such tax cuts influence the oil income in Russia. We find that an EU-wide tax cut of €0.20 l−1increases Russia’s oil profits by around €8 million per day in the short and long term. This is equivalent to €3,100 million per year, 0.2% of Russia’s gross domestic product or 5% of its military spending. We show that a cash transfer to EU citizens—with a fiscal burden equivalent to the tax cut—reduces these side effects to a fraction.
Removing unwanted variation from large-scale RNA sequencing data with PRPS
Authors: Molania Ramyar , Foroutan Momeneh , Gagnon-Bartsch Johann A. , Gandolfo Luke C. , Jain Aryan , Sinha Abhishek , Olshansky Gavriel , Dobrovic Alexander , Papenfuss Anthony T. , Speed Terence P. 
Published: September 2022

AbstractAccurate identification and effective removal of unwanted variation is essential to derive meaningful biological results from RNA sequencing (RNA-seq) data, especially when the data come from large and complex studies. Using RNA-seq data from The Cancer Genome Atlas (TCGA), we examined several sources of unwanted variation and demonstrate here how these can significantly compromise various downstream analyses, including cancer subtype identification, association between gene expression and survival outcomes and gene co-expression analysis. We propose a strategy, called pseudo-replicates of pseudo-samples (PRPS), for deploying our recently developed normalization method, called removing unwanted variation III (RUV-III), to remove the variation caused by library size, tumor purity and batch effects in TCGA RNA-seq data. We illustrate the value of our approach by comparing it to the standard TCGA normalizations on several TCGA RNA-seq datasets. RUV-III with PRPS can be used to integrate and normalize other large transcriptomic datasets coming from multiple laboratories or platforms.
Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing
Authors: Simmons Sean K. , Lithwick-Yanai Gila , Adiconis Xian , Oberstrass Florian , Iremadze Nika , Geiger-Schuller Kathryn , Thakore Pratiksha I. , Frangieh Chris J. , Barad Omer , Almogy Gilad , Rozenblatt-Rosen Orit , Regev Aviv , Lipson Doron , Levin Joshua Z. 
Published: September 2022

AbstractHere we introduce a mostly natural sequencing-by-synthesis (mnSBS) method for single-cell RNA sequencing (scRNA-seq), adapted to the Ultima genomics platform, and systematically benchmark it against current scRNA-seq technology. mnSBS uses mostly natural, unmodified nucleotides and only a low fraction of fluorescently labeled nucleotides, which allows for high polymerase processivity and lower costs. We demonstrate successful application in four scRNA-seq case studies of different technical and biological types, including 5′ and 3′ scRNA-seq, human peripheral blood mononuclear cells from a single individual and in multiplex, as well as Perturb-Seq. Benchmarking shows that results from mnSBS-based scRNA-seq are very similar to those using Illumina sequencing, with minor differences in results related to the position of reads relative to annotated gene boundaries, owing to single-end reads of Ultima being closer to gene ends than reads from Illumina. The method is thus compatible with state-of-the-art scRNA-seq libraries independent of the sequencing technology. We expect mnSBS to be of particular utility for cost-effective large-scale scRNA-seq projects.