bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasmodium falciparumgenetic diversity in coincident human and mosquito hosts
Authors: Lapp Zena , Obala Andrew A , Abel Lucy , Rasmussen David A , Sumner Kelsey M , Freedman Elizabeth , Taylor Steve M , Prudhomme-O’Meara Wendy 
Published: May 2022

AbstractPopulation genetic diversity ofP. falciparumantigenic loci is high despite large bottlenecks in population size during the parasite life cycle. The extent of this diversity in human blood-stage infections, following expansion from a small number of liver-stage schizonts, has been well described. However, little is known about parasite genetic diversity in the vector, where a similar bottleneck and expansion occurs following parasite mating and where parasite genotypes from several different human infections may accumulate. We assessed parasite genetic diversity within human and mosquitoP. falciparuminfections collected from the same households during a 14-month longitudinal cohort study using amplicon deep sequencing of two antigenic gene fragments (ama1andcsp). To a prior set of infected humans (n=1175/2813; 86.2% sequencing success) and mosquito abdomens (n=199/1448; 95.5% sequencing success), we added sequences from infected mosquito heads (n=134/1448; 98.5% sequencing success). Across all sample types we observed 456ama1and 289cspunique haplotypes. While both hosts contained many rare haplotypes, population genetic metrics indicated that the overall and sample-level parasite populations were more diverse in mosquitoes than in humans, and infections were more likely to harbor a dominant haplotype in humans than in mosquitoes (based on relative read abundance). Finally, within a given mosquito there was little overlap in genetic composition of abdomen and head infections, suggesting that infections may be cleared from the abdomen during a mosquito’s lifespan. Taken together, our observations provide evidence for the role of the mosquito vector in maintaining sequence diversity of malaria parasite populations.Significance statementConcurrent infections with multiple strains ofPlasmodium falciparum, the leading causative agent of death due to malaria, are common in highly endemic regions. During transitions within and between the parasite’s mosquito and human hosts, population bottlenecks occur, and distinct parasite strains may have differential fitness in the various environments encountered. These bottlenecks and fitness differences may lead to differences in strain prevalence and diversity between hosts. We investigated differences in genetic diversity betweenP. falciparumparasites in human and mosquito hosts and found that, compared to human parasite populations and infections, mosquito populations and infections were more diverse. This suggests that the mosquito vector may play a role in in maintaining sequence diversity in malaria parasite populations.
Dual-wavelength stopped-flow analysis of the lateral and longitudinal assembly kinetics of vimentin
Authors: Schween Lovis , Mücke Norbert , Portet Stéphanie , Goldmann Wolfgang H. , Herrmann Harald , Fabry Ben 
Published: May 2022

Vimentin is a highly charged intermediate filament protein that inherently forms extended dimeric coiled-coils, which serve as the basic building blocks of intermediate filaments. Under low ionic strength conditions, vimentin filaments dissociate into uniform tetrameric complexes of two anti-parallel oriented, half-staggered coiled-coil dimers. By addition of salt, vimentin tetramers spontaneously reassemble into filaments in a time-dependent process: i) lateral assembly of tetramers into unit-length filaments (ULFs); ii) longitudinal annealing of ULFs; iii) longitudinal assembly of filaments coupled with subsequent radial compaction. To independently determine the lateral and longitudinal assembly kinetics, we measure with a stopped-flow instrument the static light scattering signal at two different wavelengths (405 and 594 nm) with a temporal resolution of 3 ms, and analyze the signals based on Rayleigh-Gans theory. This theory considers that the intensity of the scattered light depends not only on the molecular weight of the scattering object but also on its shape. This shape-dependence is more pronounced at shorter wavelengths, allowing us to decompose the scattered light signal into its components arising from lateral and longitudinal filament assembly. We demonstrate that both the lateral and longitudinal filament assembly kinetics increase with salt concentration.Significance statementThe proper formation of intermediate filament (IF) networks in the cytoplasm is important for numerous cell functions. Here, we present a stopped-flow method for measuring the in-vitro assembly kinetics of intermediate filaments with a temporal resolution of 3 ms using static light scattering at two different wavelengths. This allows us to compute the shape factor of the assembly products based on Rayleigh-Gans light scattering theory. From the shape factor, we can separately measure the lateral assembly of tetramers into unit-length filaments (ULFs), and the longitudinal annealing of ULFs and longer filaments. For the IF protein vimentin, we find that with increasing salt concentrations, both the lateral and longitudinal assembly rates increase, and unstable, hyper-aggregated assembly complexes emerge.
An endolysosome membrane transformation process for engulfment of autophagosomes independently of ESCRT
Authors: Wu Zhe , Wang Yufen , Wang Chuangpeng , Liu Fengping , Rong Yueguang 
Published: May 2022

AbstractEndolysosome, usually regarded as the cellular recycling bin, digests materials from multiple origins. The materials from different origins are delivered to endolysosome through the vesicle fusion, kiss-and-run mechanisms, CMA or microautophagy. However, it remains unknown whether endolysosome can receive cargo via other ways. Here, we reported another endolysosomal process for receiving materials. In this, endolysosome receives materials by extending two arms which embraces autophagosomes and engulfs the autophagosomes ultimately, but not via the conventional fusion of autophagosomes with endolysosomes. We named this process as LEA (lysosome eats autophagosome) and the endolysosome arms as LF (endolysosome filopodia) provisionally. The endolysosomes with engulfed autophagosomes (LEA endolysosomes) get more autophagosomes via the fusion with other LEA endolysosomes. The engulfed autophagosomes are labelled by F-actin on their membranes and have ER protein Sec61β and peroxisome protein Pex14 inside their lumens, but mitochondria are excluded outside endolysosome. Our discovery of LEA and LF reveal an unidentified endolysosome transformation process which is responsible for receiving cargoes.
Senescent Preosteoclast Secretome Promotes Metabolic Syndrome-Associated Osteoarthritis through Cyclooxygenase 2
Authors: Su Weiping , Liu Guanqiao , Mohajer Bahram , Wang Jiekang , Shen Alena , Zhang Weixin , Liu Bin , Guermazi Ali , Gao Peisong , Cao Xu , Demehri Shadpour , Wan Mei 
Published: May 2022

AbstractBackgroundMetabolic syndrome–associated osteoarthritis (MetS-OA) is a distinct osteoarthritis phenotype defined by the coexistence of MetS or its individual components. Despite the high prevalence of MetS-OA, its pathogenic mechanisms are unclear. The aim of this study was to determine the role of cellular senescence in the development of MetS-OA.MethodsAnalysis of the human osteoarthritis initiative (OAI) dataset was conducted to investigate the MRI subchondral bone features of MetS-human OA participants. Joint phenotype and senescent cells were evaluated in two MetS-OA mouse models: high-fat diet (HFD)-challenged mice and STR/Ort mice. In addition, the molecular mechanisms by which preosteoclasts become senescent as well as how the senescent preosteoclasts impair subchondral bone microenvironment were characterized usingin vitropreosteoclast culture system.ResultsHumans and mice with MetS are more likely to develop osteoarthritis-related subchondral bone alterations than those without MetS. MetS-OA mice exhibited a rapid increase in joint subchondral bone plate and trabecular thickness before articular cartilage degeneration. Subchondral preosteoclasts undergo senescence at the pre- or early-osteoarthritis stage and acquire a unique secretome to stimulate osteoblast differentiation and inhibit osteoclast differentiation. Antagonizing preosteoclast senescence markedly mitigates pathological subchondral alterations and osteoarthritis progression in MetS-OA mice. At the molecular level, preosteoclast secretome activates COX2-PGE2, resulting in stimulated differentiation of osteoblast progenitors for subchondral bone formation. Administration of a selective COX2 inhibitor attenuated subchondral bone alteration and osteoarthritis progression in MetS-OA mice. Longitudinal analyses of the human Osteoarthritis Initiative (OAI) cohort dataset also revealed that COX2 inhibitor use, relative to non-selective nonsteroidal anti-inflammatory drug use, is associated with less progression of osteoarthritis and subchondral bone marrow lesion worsening in participants with MetS-OA.ConclusionsOur findings suggest a central role of a senescent preosteoclast secretome-COX2/PGE2 axis in the pathogenesis of MetS-OA, in which selective COX2 inhibitors may have disease-modifying potential.FundingThis work was supported by the National Institutes of Health grant R01AG068226 and R01AG072090 to M.W., R01AR079620 to S.D., and P01AG066603 to X.C.
Location of competitive facilities: a comprehensive review and future research agenda
Authors: Mishra Mamta , Singh Surya Prakash , Gupta M. P. 
Published: May 2022

PurposeThe research in competitive facility location (CFL) is quite dynamic, both from a problem formulation and an algorithmic point of view. Research direction has changed immensely over the years to address various competitive challenges. This study aims to explore CFL literature to highlight these research trends, important issues and future research opportunities.Design/methodology/approachThis study utilises the Scopus database to search for related CFL models and adopts a five-step systematic approach for the review process. The five steps involve (1) Article Identification and keyword selection, (2) Selection criteria, (3) Literature review, (4) Literature analysis and (5) Research studies.FindingsThe paper presents a comprehensive review of CFL modelling efforts from 1981 to 2021 to provide a depth study of the research evolution in this area. The published articles are classified based on multiple characteristics, including the type of problem, type of competition, game-theoretical approaches, customer behaviour, decision space, type of demand, number of facilities, capacity and budget limitations. The review also highlights the popular problem areas and dedicated research in the respective domain. In addition, a second classification is also provided based on solution methods adopted to solve various CFL models and real-world case studies.Originality/valueThe paper covers 40 years of CFL literature from the perspective of the problem area, CFL characteristics and the solution approach. Additionally, it introduces characteristics such as capacity limit and budget constraint for the first time for classification purposes.
Meanings of management in the post-socialist higher education: the case of Lithuania
Authors: Rybnikova Irma , Lang Rainhart 
Published: May 2022

PurposeThe study examines prevailing understandings and meanings of management represented in Lithuanian higher management education as a selected East European post-socialist country. While concentrating on the notion of management as an institution, the study draws upon the concept of “travel of ideas” by Czarniawska (2008). The study aims at analysing what reasons led to the development of specific management meanings in Lithuanian higher education by considering local historical and socio-political processes of transformation.Design/methodology/approachThe empirical analysis is based on a qualitative content analysis of 23 relevant textbooks on general management in Lithuania, written by local authors.FindingsThe results show that the reception and translation of management concepts in Lithuania is mainly focused on rationalistic Anglo-Saxon concepts. Dominating meanings are informed by Taylorism, with alternative or critical management approaches remaining absent. The study additionally reveals the effect of “imprinting”, referring to the fact that local management pioneers have a considerable and sustainable impact on how and which management models and theories are adopted in management education.Research limitations/implicationsSince the analysis is limited to local textbooks in higher education of only one post-socialist country, there is a need for further research, especially for the inclusion of the remaining Central and Eastern European (CEE) countries. Additionally, other factors that may influence management meanings in higher education in post-socialist countries need to be considered by analysing translated books, management programmes or guest lecturers from other countries.Practical implicationsOne of the central implications of the research into management in higher education is the call for a stronger inclusion of management ideas that go beyond Taylorist approaches. These could be team-based management or participative management, as these potentially better align with work-oriented expectations by prospective, especially young, employees.Originality/valueThe study shows the relevance of historic roots regarding managerial thought. The study identifies an “imprinting” by local pioneers in the field of management that establishes initial and enduring meanings of management in a considered country. The mechanism of “imprinting” serves as a selective filter and indicates a local general receptivity towards certain ideas and could, thus, enable or impede travelling of ideas. In the case of Lithuanian higher management education, the study shows that “imprinting” has led to a narrow meaning of management with a higher receptivity towards concepts and approaches that align with Taylor's scientific management theory.
Why do firms purchase directors' and officers' liability insurance? – perspective from economic policy uncertainty
Authors: Jiang Haiyan , Jia Jing , Hu Yuanyuan 
Published: May 2022

PurposeThis study aims to investigate whether firms purchase directors' and officers' liability (D&O) insurance when the country-level economic policy uncertainty (EPU) is high.Design/methodology/approachThis study uses D&O insurance data from Chinese listed firms between 2003 and 2019 to conduct regression analyses to examine the association between D&O insurance and EPU.FindingsThe results show that government EPU, despite being an exogenous factor, increases the likelihood of firms' purchasing D&O insurance, and this effect is more pronounced when firms are exposed to great share price crash risk and high litigation risk, suggesting that firms intend to purchase D&O insurance possibly due to the accentuated stock price crash risk and litigation risk associated with EPU. In addition, the results indicate that the effect of EPU on the D&O insurance purchase decision is moderated by the provincial capital market development and internal control quality.Practical implicationsThe study highlights the role of uncertain economic policies in shareholder approval of D&O insurance purchases.Originality/valueThe study enriches the literature on the determinants of D&O insurance purchases by documenting novel evidence that country-level EPU is a key institutional factor shaping firms' decisions to purchase D&O insurance.
MITTI Café: enabling disability inclusion in India through scalable business model
Authors: Moore Amy Fisher , Toefy Tracey 
Published: May 2022

Study level/applicability
The case can be used at undergraduate and postgraduate level, in management development programmes or in Executive Education programmes.

Subject area
Social entrepreneurship, social inclusion, business model innovation, sustainability, strategy design and strategy execution.

Case overview
The case explores the development of MITTI Café, an organisation that trains and employs individuals with intellectual, physical and/or psychiatric disabilities to work in inclusive kitchens and cafes in India. The protagonist is the founder of the café, Alina Alam, who has won several international awards for her work. The case highlights Alam’s approach and how she is trying to challenge societal and business perspectives relating to disability. From 2017 to 2021, Alam has scaled and operationalised the business, building her core team, leveraging several partnerships with stakeholders and putting into place offerings, processes and procedures that created a sustainable business model and blueprint.MITTI Café aligns itself with several of the Sustainable Development Goals (SDGs), with sustainability and social impact at the core of its strategy. As Alam considers the future in July 2021, what else needs to be taken into consideration to scale either within India or abroad?

Expected learning outcomes
Following reading and exploring the case, students should be able to identify how social exclusion and inclusion manifests in a business context, and how social entrepreneurship ventures such as MITTI Café can address this challenge; identify capabilities in the context of people with disabilities; recognise how stakeholder relationships can be leveraged as a force for good and for growth, and address SDGs through social enterprise; identify and categorise resources and capabilities within organisations; evaluate opportunities for growth and scale.

Social implications
The case explores how the protagonist is challenging the concept of “ability” and through her work with the differently abled providing scalable opportunities for social inclusion and dignity.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 11: Strategy.
Digital supply chain transformation at Jabil: integrated supply and demand planning via concurrent planning
Authors: Keskin Burcu 
Published: May 2022

Study level/applicability
The case would be relevant to undergraduate level or an introductory master's level course in operations management (OM), supply chain management and production.

Subject area
The case can be used as part of a core OM course in the MBA curriculum or any OM or supply chain elective.

Case overview
As a highly diversified manufacturing services company, Jabil's S&OP solution supports customers across many industries such as automotive, cloud computing, consumer packaging, healthcare, mobile, retail and telecommunications. Jabil's customers expect a rapid and accurate response to their demand within hours. Previously, Jabil used a series of legacy disconnected planning tools, unsynchronized data required time-consuming manipulation with Excel. Processes were conducted in siloes leading to a “load and chase” approach, which resulted in excess inventory, component shortages and inadequate capacity. The case focuses on one of the Jabil executives, Lizet Tymon (she). Struggling with the issues caused by the disconnected planning tools, Lizet champions implementing a fully integrated suite of services (built on top of the Kinaxis' RapidResponse software platform). The technology solution proposed by Lizet was ultimately implemented across the company, and the project received high marks, and it opened up career opportunities for her. However, it was not a smooth ride at the very beginning. The case focuses on the issues experienced by Lizet, as she is introducing a new technological solution approach and trying to earn support from her team, her peers, her immediate supervisor, her customers and her higher-level executives.

Expected learning outcomes
The teaching objectives include: understanding and appreciating the supply chain complexities experienced by a global contract manufacturer; helping students think critically regarding the issues around the sales and ops planning; identifying the data needs for the operation and management of a worldwide, connected supply chain; investigating agile solution approaches for information sharing, decision-making and decision-sharing; and exposing the challenges associated with a large-scale technology adaptation.

Social Implications
This case study describes the supply chain challenges experienced by a global manufacturing solutions provider and illustrates the technology adaptation led by a female executive.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 9: Operations and Logistics
How to explain AI systems to end users: a systematic literature review and research agenda
Authors: Laato Samuli , Tiainen Miika , Najmul Islam A.K.M. , Mäntymäki Matti 
Published: May 2022

PurposeInscrutable machine learning (ML) models are part of increasingly many information systems. Understanding how these models behave, and what their output is based on, is a challenge for developers let alone non-technical end users.Design/methodology/approachThe authors investigate how AI systems and their decisions ought to be explained for end users through a systematic literature review.FindingsThe authors’ synthesis of the literature suggests that AI system communication for end users has five high-level goals: (1) understandability, (2) trustworthiness, (3) transparency, (4) controllability and (5) fairness. The authors identified several design recommendations, such as offering personalized and on-demand explanations and focusing on the explainability of key functionalities instead of aiming to explain the whole system. There exists multiple trade-offs in AI system explanations, and there is no single best solution that fits all cases.Research limitations/implicationsBased on the synthesis, the authors provide a design framework for explaining AI systems to end users. The study contributes to the work on AI governance by suggesting guidelines on how to make AI systems more understandable, fair, trustworthy, controllable and transparent.Originality/valueThis literature review brings together the literature on AI system communication and explainable AI (XAI) for end users. Building on previous academic literature on the topic, it provides synthesized insights, design recommendations and future research agenda.
Surviving disruption: nature inspired solutions
Authors: Swain Sydney , Brahimi Nadjib 
Published: May 2022

PurposeDecision-makers in companies increasingly face unprecedented natural disasters. When business continuity is at risk, managers need a framework to imminently react.Design/methodology/approachA literature review and analysis of survival responses in nature and business case examples of company responses to the Covid-19 pandemic was the approach used.FindingsThere are direct parallels between the physiological stress response when a living individual perceives a threat to its survival, and the immediate reactions that occur when companies are faced with a disruptive event.Practical implicationsThis article is meant to be used by decision-makers in companies to better react to disruptive events.Originality/valueWhile nature-inspired methods have inspired inventions and algorithms, Hans Selye's general adaptation theory has not been used in parallel with business scenarios. We correlate fundamental organism survival mechanisms with a risk response framework to improve the probability of business survival during external threats.