bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pro-efferocytic macrophage membrane biomimetic nanoparticles for the synergistic treatment of atherosclerosis via competition effect
Authors: Sha Xuan , Dai Yue , Chong Lijuan , Wei Min , Xing Mengyuan , Zhang Chun , Li Jingjing 
Published: December 2022

AbstractMacrophages participate in many links in the pathological process of atherosclerosis (AS) and the regulation of influence of macrophages at the molecular level might be a new avenue for AS treatment. For this aim, the macrophage membrane biomimetic nanoparticles, derived from macrophage membrane coated SHP1i-loaded liposome NPs (MM@Lips-SHP1i) was designed. Due to the reservation of intrinsic membrane proteins and function from macrophages, the biomimic nanoparticles could effectively evade clearance by the immune system, prolong blood circulation time and actively tend and aggregate to atherosclerotic plaques. More importantly, in the plaque area, MM@Lips-SHP1i nanoparticles could compete with macrophages in vivo to bind with oxidized low-density lipoprotein (oxLDL) and lipopolysaccharide (LPS), reduce uptake of new lipids by macrophages, reduce foam cell formation, and inhibit the expression of pro-inflammatory cytokines. In addition, small molecule inhibitor of SHP-1, the downstream effector molecule of CD47 loaded in macrophage membrane biomimetic nanoparticles could interrupt CD47-SIRPα signal transduction in monocytes and macrophages, thereby enhancing the efferocytosis of macrophages, inhibiting the progression of plaque, achieving synergistic treatment of atherosclerosis. This work focuses on the key process in the formation of AS, macrophage foaming and chronic inflammation, and is based on the fact that macrophage membrane biomimetic nanoparticles can preserve the key surface proteins of macrophages closely related to the formation of AS, providing a new avenue to inhibit the progression of AS by utilizing the biological characteristics of macrophage membrane in macrophage membrane biomimetic nanoparticles.
Assessing feasibility, construct validity, and reliability of a new aged care-specific preference-based quality of life instrument: evidence from older Australians in residential aged care
Authors: Khadka J , Hutchinson C , Milte R , Cleland J , Muller A , Bowes N , Ratcliffe J 
Published: December 2022

Abstract
Background
Quality of Life-Aged Care Consumers (QOL-ACC) is a new older-person-specific quality of life instrument designed for application in quality assessment and economic evaluation in aged care. The QOL-ACC was designed from its inception with older people receiving aged care services ensuring its strong content validity. Given that the QOL-ACC has already been validated in home care settings and a preference-weighted value set developed, we aimed to assess feasibility, construct validity and reliability of the QOL-ACC in residential aged care settings.

Methods
Individuals living in residential aged care facilities participated in an interviewer-facilitated survey. The survey included the QOL-ACC, QCE-ACC (quality of aged care experience measure) and two other preference-based quality of life instruments (ASCOT and EQ-5D-5L). Feasibility was assessed using missing data and ceiling/floor effects. Construct validity was assessed by exploring the relationship between the QOL-ACC and other instruments (convergent validity) and the QOL-ACC’s ability to discriminate varying levels of self-rated health and quality of life. Internal consistency reliability was assessed using Cronbach’s alpha (α).

Results
Of the 200 residents (mean age, 85 ± 7.7 years) who completed the survey, 60% were female and 69% were born in Australia. One in three participating residents self-rated their health as fair/poor. The QOL-ACC had no missing data but had small floor effects (0.5%) and acceptable ceiling effects (7.5%). It demonstrated moderate correlation with ASCOT (r = 0.51, p < 0.001) and EQ-5D-5L (r = 0.52, p < 0.001) and a stronger correlation with the QCE-ACC (r = 0.57, p < 0.001). Residents with poor self-rated health and quality of life had significantly lower scores on the QOL-ACC. The internal consistency reliability of the QOL-ACC and its dimensions was good (α = 0.70–0.77).

Conclusions
The QOL-ACC demonstrated good feasibility, construct validity and internal consistency reliability to assess aged care-related quality of life. Moderate correlations of the QOL-ACC and other instruments provide evidence of its construct validity and signifies that the QOL-ACC adds non-redundant and non-interchangeable information beyond the existing instruments. A stronger correlation with the QCE-ACC than other instruments may indicate that quality of life is more intimately connected with the care experience than either health- or social-related quality of life in residential aged care settings.
Identifying hepatocellular carcinoma patients with survival benefits from surgery combined with chemotherapy: based on machine learning model
Authors: Hu Jie , Gong Ni , Li Dan , Deng Youyuan , Chen Jiawei , Luo Dingan , Zhou Wei , Xu Ke 
Published: December 2022

Abstract
Background
Hepatocellular carcinoma (HCC) is still fatal even after surgical resection. The purpose of this study was to analyze the prognostic factors of 5-year survival rate and to establish a model to identify HCC patients with gain of surgery combined with chemotherapy.

Methods
All patients with HCC after surgery from January 2010 to December 2015 were selected from the Surveillance, Epidemiology, and End Results (SEER) database. Univariate and multivariate logistic analysis were used to analyze the prognostic factors of patients, and the risk prediction model of 5-year survival rate of HCC patients was established by classical decision tree method. Propensity score matching was used to eliminate the confounding factors of whether to receive chemotherapy in high-risk group or low-risk group.

Results
One-thousand six-hundred twenty-five eligible HCC patients were included in the study. Marital status, α-fetoprotein (AFP), vascular infiltration, tumor size, number of lesions, and grade were independent prognostic factors affecting the 5-year survival rate of HCC patients. The area under the curve of the 5-year survival risk prediction model constructed from the above variables was 0.76, and the classification accuracy, precision, recall, and F1 scores were 0.752, 0.83, 0.842, and 0.836, respectively. High-risk patients classified according to the prediction model had better 5-year survival rate after chemotherapy, while there was no difference in 5-year survival rate between patients receiving chemotherapy and patients not receiving chemotherapy in the low-risk group.

Conclusions
The 5-year survival risk prediction model constructed in this study provides accurate survival prediction information. The high-risk patients determined according to the prediction model may benefit from the 5-year survival rate after combined chemotherapy.
Two phenotypes of chronic recurrent multifocal osteomyelitis with different patterns of bone involvement
Authors: Cebecauerová Dita , Malcová Hana , Koukolská Veronika , Kvíčalová Zuzana , Souček Ondřej , Wagenknecht Lukáš , Bronský Jiří , Šumník Zdeněk , Kynčl Martin , Cebecauer Marek , Horváth Rudolf 
Published: December 2022

Abstract
Introduction
Chronic Recurrent Multifocal Osteomyelitis (CRMO) is an autoinflammatory bone disorder with predominantly paediatric onset. Children present with multifocal osteolytic lesions accompanied by bone pain and soft tissue swelling. Patients often exhibit extraosseous co-morbidities such as psoriasis, inflammatory bowel disease, and arthritis.

Objectives
Comparison of children with two different phenotypes of CRMO defined by presence or absence of extraosseous co-morbidities.

Methods
Children diagnosed with CRMO at the Motol University Hospital between 2010 and 2020 were retrospectively reviewed, and according to the absence or presence of extraosseous manifestations divided into two cohorts – bone limited CRMO and complex CRMO. The two groups were compared in terms of demographic data, age at disease onset, number and site of bone lesions, laboratory biomarker values, and need of escalation to a second-line therapy.

Results
Thirty-seven children (30 female, 7 male) with confirmed CRMO were included in the analysis. The mean age at disease onset was 10 years. All but 3 patients presented with multifocal disease. Twenty-three children (62%) had at least one extraosseous manifestation (13 sacroiliitis, 8 inflammatory bowel disease, 6 skin disease [acne, pustulosis, or psoriasis], 7 arthritis). Complex CRMO was associated with a significantly higher ESR rate (p = 0.0064) and CRP level (p = 0.018). The groups did not differ in number of foci or in age at disease onset. Bone lesion distribution differed between the two groups with significantly more frequent involvement of clavicle (p = 0.011) and pelvis (p = 0.038) in patients with complex CRMO. Children with complex CRMO more often needed escalation of therapy to DMARDs and biologic agents.

Conclusion
Our data suggest that CRMO affecting solely the skeleton has milder course compared to complex CRMO with extraskeletal features. Further studies are needed to explore the clinical as well as the patient reported outcomes and promote individually tailored therapeutic strategies in both CRMO phenotypes.
Effect of ovarian growth factors on ultra-structural maturation in frozen human immature oocytes after in vitro maturation: a comparative study
Authors: Akbari Hakimeh , Mohammadi Masoud 
Published: December 2022

Abstract
Background
In artificial reproductive technique (ART), nearly 20% of human oocytes are immature in the germinal vesicle (GV) phase. Consequently, the best method for reserving them is cryopreserving GV oocytes, and in vitro maturation (IVM) is recommended. The aim of this study was to determine the ultrastructure characteristics of fresh and vitrified immature human oocytes after in vitro maturation in conditioned mediums.

Methods
This study was a comparative laboratory study carried out in 2018 at Afzalipur Infertility Center in Kerman. 170 fresh and 198 vitrified GV oocytes were cultured within three IVM mediums; α-mem as control medium, α-mem supplemented with human bone marrow mesenchymal stem cells (BM-MSCs) and α-mem supplemented with ovarian growth factors (O.F). After 48 h, the maturation rate and morphological feature of IVM oocytes [132 fresh IVM (fIVM) and 134 vitrified IVM (vIVM)] were evaluated. For the ultrastructure study, 10 IVM oocytes from each medium were compared with 10 fresh in vivo oocytes cancelled from ART.

Results
The survival rate of vitrified GV oocyte after thawing was 88.88%. The oocyte maturation rate was reduced in vIVM compared to the fIVM group (76.33% vs. 77.95%); the highest oocyte maturation rate in the O.F fIVM and lowest in α-mem vIVM (82.35% vs. 71.42%). The lowest number of cortical granules was observed in α-mem vIVM, but the greatest presence of M-SER aggregates was in O.F fIVM. In vIVM oocytes, the oolemma contained irregular little microvillus organization.

Conclusions
The O.F mediums have shown the highest maturation which defends the oocyte ultra-structural conservation.
Factors associated with HIV self-testing among female university students in Uganda: a cross-sectional study
Authors: Segawa Ivan , Bakeera-Kitaka Sabrina , Ssebambulidde Kenneth , Muwonge Timothy R. , Oriokot Lorraine , Ojiambo Kevin Ouma , Mujugira Andrew 
Published: December 2022

Abstract
Background
Adolescent girls and young women (AGYW) at institutions of higher learning are at high risk of HIV, and conventional HIV testing services may not reach them sufficiently. HIV self-testing (HIVST) scalability can be informed by identifying AGYW who have used or are interested in using HIVST. We aimed to determine factors associated with use and willingness to use HIVST among female university students.

Methods
An online cross-sectional survey was conducted among 483 female students at Makerere University, Uganda. Proportions of students who have used or are willing to use HIVST and their associated factors were determined. Modified Poisson regression models were used to estimate prevalence ratios (PR) and their 95% confidence intervals (CI).

Results
The median age of the participants was 22 (Interquartile range [IQR] 21–23) years, and 21% had never tested for HIV. Over 93% were willing to utilize HIVST, and 19% had ever used HIV self-test kits. Increasing age (adjusted prevalence ratio [aPR] 1.23 per year, 95% CI 1.07–1.43) was significantly associated with HIVST use. Predictors of willingness to self-test for HIV were college type (arts vs. science-based, aPR 0.92, 95% CI 0.88–0.97), number of sexual partners (one, aPR 1.07, 95% CI 1.03–1.12 or ≥ 2, aPR 1.08, 95% CI 1.04–1.19, vs. none), alcohol (aPR 1.04, 95% CI: 1.00–1.09) or injection drug (aPR 1.04, 95% CI 1.00–1.09) use, a history of sexually transmitted infections in past 12 months (aPR 1.05, 95% CI 1.01–1.09), and HIV testing experience (tested in past 12 months, aPR 1.12, 95% CI 1.02–1.22 or over 12 months, aPR 1.13, 95% CI 1.03–1.24, vs. never tested).

Conclusion
HIVST was highly acceptable despite its limited use. This study demonstrates female student characteristics that can be leveraged to scale up HIVST programs in higher institutions of learning.
Drug synergy discovery of tavaborole and aminoglycosides against Escherichia coli using high throughput screening
Authors: Liu Shasha , She Pengfei , Li Zehao , Li Yimin , Li Linhui , Yang Yifan , Zhou Linying , Wu Yong 
Published: December 2022

AbstractHigh incidences of urinary tract infection (UTI) of aminoglycosides-resistant E.coli causes a severe burden for public health. A new therapeutic strategy to ease this crisis is to repurpose non-antibacterial compounds to increase aminoglycosides sensibility against multidrug resistant E.coli pathogens. Based on high throughput screening technology, we profile the antimicrobial activity of tavaborole, a first antifungal benzoxaborole drug for onychomycosis treatment, and investigate the synergistic interaction between tavaborole and aminoglycosides, especially tobramycin and amikacin. Most importantly, by resistance accumulation assay, we found that, tavaborole not only slowed resistance occurrence of aminoglycosides, but also reduced invasiveness of E.coli in combination with tobramycin. Mechanistic studies preliminary explored that tavaborole and aminoglycosides lead to mistranslation, but would be still necessary to investigate more details for further research. In addition, tavaborole exhibited low systematic toxicity in vitro and in vivo, and enhanced aminoglycoside bactericidal activity in mice peritonitis model. Collectively, these results suggest the potential of tavaborole as a novel aminoglycosides adjuvant to tackle the clinically relevant drug resistant E. coli and encourages us to discover more benzoxaborole analogues for circumvention of recalcitrant infections.
Proteomic signatures of schizophrenia-sourced iPSC-derived neural cells and brain organoids are similar to patients' postmortem brains
Authors: Nascimento Juliana Minardi , Saia-Cereda Verônica M. , Zuccoli Giuliana S. , Reis-de-Oliveira Guilherme , Carregari Victor Corasolla , Smith Bradley J. , Rehen Stevens K. , Martins-de-Souza Daniel 
Published: December 2022

Abstract
Background
Schizophrenia is a complex and severe neuropsychiatric disorder, with a wide range of debilitating symptoms. Several aspects of its multifactorial complexity are still unknown, and some are accepted to be an early developmental deficiency with a more specifically neurodevelopmental origin. Understanding the timepoints of disturbances during neural cell differentiation processes could lead to an insight into the development of the disorder. In this context, human brain organoids and neural cells differentiated from patient-derived induced pluripotent stem cells are of great interest as a model to study the developmental origins of the disease.

Results
Here we evaluated the differential expression of proteins of schizophrenia patient-derived neural progenitors (NPCs), early neurons, and brain organoids in comparison to healthy individuals. Using bottom-up shotgun proteomics with a label-free approach for quantitative analysis, we found multiple dysregulated proteins since NPCs, modified, and disrupted the 21DIV neuronal differentiation, and cerebral organoids. Our experimental methods have shown impairments in pathways never before found in patient-derived induced pluripotent stem cells studies, such as spliceosomes and amino acid metabolism; but also, those such as axonal guidance and synaptogenesis, in line with postmortem tissue studies of schizophrenia patients.

Conclusion
In conclusion, here we provide comprehensive, large-scale, protein-level data of different neural cell models that may uncover early events in brain development, underlying several of the mechanisms within the origins of schizophrenia.
Solving nonlinear and dynamic programming equations on extended b-metric spaces with the fixed-point technique
Authors: Belhenniche Abdelkader , Guran Liliana , Benahmed Sfya , Lobo Pereira Fernando 
Published: December 2022

AbstractIn this article, we present an approach to solve a wide range of nonlinear equations formulated in extended b-metric spaces based on a new fixed-point theorem on these spaces. This research effort was motivated by challenges arising in solving pattern problems efficiently that can not be addressed by using standard metric spaces. Our approach relies on a novel common fixed-point theorem for Ćirić-type operators on extended b-metric spaces requiring only very weak assumptions that we present and derive in this article. The proposed approach is illustrated by applications asserting the existence and uniqueness of the solutions to Bellman equations, Volterra integral equations, and fractional differential equations formulated in extended b-metric spaces. Moreover, the obtained results provide general constructive recursive procedures to solve the above types of nonlinear equations.
A 12-week consumer wearable activity tracker-based intervention reduces sedentary behaviour and improves cardiometabolic health in free-living sedentary adults: a randomised controlled trial
Authors: Franssen Wouter M. A. , Nieste Ine , Vandereyt Frank , Savelberg Hans H. C. M. , Eijnde Bert O. 
Published: December 2022

AbstractBackgroundReducing sedentary behaviour significantly improves cardiometabolic health and plays an important role in the prevention and management of cardiometabolic diseases. However, limited effective strategies have been proposed to combat the negative effects of sedentary lifestyles. Although consumer wearable activity trackers (CWATs) can effectively improve physical activity, they were only included as part of a multiple behaviour change technique. In addition, it is not known whether these devices are also effective to reduce sedentary behaviour. Therefore, we aim to investigate the efficacy of a single component CWAT-only intervention and the added value of a multicomponent (CWATs + motivational interviewing) behaviour change intervention to reduce sedentary behaviour and increase physical activity within sedentary adults.MethodsIn a three-armed randomised controlled trial, 59 (male/female: 21/38) sedentary adults were randomly allocated to a control group (n = 20), a CWAT-only group (n = 20) or the CWAT + group (CWAT + motivational interviewing; n = 19) for 12 weeks. Physical activity and sedentary behaviour were assessed using the activPAL3™ accelerometer. In addition, anthropometrics, blood pressure, plasma lipids and insulin sensitivity using an oral glucose tolerance test were assessed at baseline and after the 12-week intervention period.ResultsAs compared with the control group, the CWAT + group significantly reduced time spent in sedentary behaviour (− 81 min/day, confidence interval [95%]: [− 151, − 12] min/day) and significantly increased step count (+ 3117 [827, 5406] steps/day), standing time (+ 62 [14, 110] min/day), light intensity PA (+ 28 [5, 50] min/day) and moderate-to-vigorous PA (+ 22 [4, 40] min/day). Body fat mass (− 1.67 [− 3.21, − 0.14] kg), percentage body fat (− 1.5 [− 2.9, − 0.1] %), triglyceride concentration (− 0.31 [− 0.62, − 0.01] mmol/l), the 2 h insulin concentration (− 181 [− 409, − 46] pmol/l), the quantitative insulin sensitivity check index (− 0.022 [− 0.043, − 0.008]) and total area under the curve of insulin (− 6464 [− 26837, − 2735] mmol/l min) were significantly reduced in the CWAT + group, compared to the control group. No significant differences within the CWAT-only group were found.ConclusionA 12-week multicomponent CWAT-based intervention (CWAT + motivational interviewing) reduces sedentary time, increases physical activity levels and improves various cardiometabolic health variables in sedentary adults, whereas self-monitoring on itself (CWAT-only group) has no beneficial effects on sedentary time.Trial registrationThe present study was registered (2018) at clinicaltrials.gov as NCT03853018.
Low-Intensity Adjuvant Chemotherapy for Breast Cancer in Older Women: Results From the Prospective Multicenter HOPE Trial
Authors: Sedrak Mina S. , Sun Can-Lan , Ji Jingran , Cohen Harvey J. , Gross Cary P. , Tew William P. , Klepin Heidi D. , Wildes Tanya M. , Dotan Efrat , Freedman Rachel A. , O'Connor Tracey , Chow Selina , Fenton Mary Ann , Moy Beverly , Chapman Andrew E. , Dale William , Katheria Vani , Kuderer Nicole M. , Lyman Gary H. , Magnuson Allison , Muss Hyman B. 
Published: December 2022

PURPOSE Older women with high-risk early breast cancer (EBC) benefit from adjuvant chemotherapy, but their treatment is frequently complicated by toxic side effects, resulting in dose reductions and delays. This makes it challenging for oncologists to maintain a relative dose intensity (RDI) ≥ 85%, as recommended for optimal curative-intent treatment. Understanding which women are at risk of receiving suboptimal RDI may inform treatment discussions and guide early, targeted supportive care or geriatric comanagement interventions. METHODS This was a prespecified secondary analysis of the HOPE trial, which enrolled women age ≥ 65 years with EBC initiating neoadjuvant or adjuvant chemotherapy. RDI was calculated as the ratio of delivered to planned chemotherapy dose intensity. The primary outcome was low RDI, defined as RDI < 85%. Multivariable logistic regression with stepwise selection was used to evaluate the association between baseline variables (demographic, clinical, and geriatric assessment) and low RDI. Survival probability was estimated using the Kaplan-Meier method, and the log-rank test was used to compare overall survival. RESULTS Three hundred twenty-two patients (median age at diagnosis, 70 years; range, 65-86 years) were included. The median follow-up was 4 years. Sixty-six patients (21%) had a low RDI. Age ≥ 76 years (odds ratio [OR], 2.57; 95% CI, 1.12 to 5.91; P = .03), lower performance status (OR, 4.32; 95% CI, 1.98 to 9.42; P < .001), and use of anthracycline-based or cyclophosphamide, methotrexate, and fluorouracil regimens (OR, 3.47; 95% CI, 1.71 to 7.05; P < .001) were associated with low RDI. The 5-year overall survival probability was 0.80 versus 0.91 in patients with RDI < 85 versus ≥ 85%, respectively (log-rank P = .02). CONCLUSION One in five older patients with EBC treated with standard chemotherapy received low RDI and had inferior survival outcomes. Older patients at risk for low RDI should be identified and targeted upfront before initiating chemotherapy.