bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantized Collision/Fusion Events of Anionic Ionosomes at a Polarized Soft Micro‐Interface
Authors: Zhang Jingcheng , Huang Linhan , Fang Taoxiong , Xiang Zhipeng , He Sijia , Peljo Pekka , Gan Shiyu , Huang Xinjian , Deng Haiqiang 
Published: October 2022

AbstractSingle‐entity collisional electrochemistry (SECE) can capture physicochemical information at the single entity level. In the present work, we systematically studied in‐situ generation and detection of single anionic ionosomes via SECE combined with a miniaturized interface between two immiscible electrolyte solutions (ITIES). Ionosome is an ionic‐bilayer encapsulated nanoscopic water cluster/droplet that carries a net charge. Discrete spiky ionic currents were observed upon collisions/fusions of individual F− or Cl−‐ionosomes with a positively polarized micro‐ITIES. This fusion process was proved to follow the bulk electrolysis model. With this method, some essential factors such as concentration and charge density of the hydrated anions, and the interfacial area, were revealed. It demonstrates that anionic ionosomes share a common theoretical framework with their counterparts (i. e., cationic ionosomes, like Li+‐ionosomes). This work will spur the advancements in a myriad of fields, including such as the colloid and interface science, micro‐ and/or nanoscale electrochemistry, and electrophysiology and brain sciences.
Reduced social attention in autism is magnified by perceptual load in naturalistic environments
Authors: Haskins Amanda J. , Mentch Jeff , Botch Thomas L. , Garcia Brenda D. , Burrows Alexandra L. , Robertson Caroline E. 
Published: October 2022

AbstractIndividuals with autism spectrum conditions (ASC) describe differences in both social cognition and sensory processing, but little is known about the causal relationship between these disparate functional domains. In the present study, we sought to understand how a core characteristic of autism—reduced social attention—is impacted by the complex multisensory signals present in real‐world environments. We tested the hypothesis that reductions in social attention associated with autism would be magnified by increasing perceptual load (e.g., motion, multisensory cues). Adult participants (N = 40; 19 ASC) explored a diverse set of 360° real‐world scenes in a naturalistic, active viewing paradigm (immersive virtual reality + eyetracking). Across three conditions, we systematically varied perceptual load while holding the social and semantic information present in each scene constant. We demonstrate that reduced social attention is not a static signature of the autistic phenotype. Rather, group differences in social attention emerged with increasing perceptual load in naturalistic environments, and the susceptibility of social attention to perceptual load predicted continuous measures of autistic traits across groups. Crucially, this pattern was specific to the social domain: we did not observe differential impacts of perceptual load on attention directed toward nonsocial semantic (i.e., object, place) information or low‐level fixation behavior (i.e., overall fixation frequency or duration). This study provides a direct link between social and sensory processing in autism. Moreover, reduced social attention may be an inaccurate characterization of autism. Instead, our results suggest that social attention in autism is better explained by “social vulnerability,” particularly to the perceptual load of real‐world environments.
BCR::ABL1 levels at first month after TKI discontinuation predict subsequent maintenance of treatment‐free remission: A study from the “GRUPPO TRIVENETO LMC”
Authors: Di Giusto Sara , Toffoletti Eleonora , Bonifacio Massimiliano , Binotto Gianni , Miggiano Maria Cristina , Calistri Elisabetta , Stulle Manuela , Ermacora Anna , Stella Rossella , Scaffidi Luigi , D'Amore Fabio , Scotton Giorgia , Griguolo Davide , De Matteis Giovanna , Bertorelle Roberta , Krampera Mauro , Semenzato Gianpietro , Fanin Renato , Damiani Daniela , Tiribelli Mario 
Published: October 2022

AbstractWe analyzed BCR::ABL1 expression at stop and in the first month after discontinuation in 168 chronic myeloid leukemia patients who stopped imatinib or 2nd generation tyrosine kinase inhibitors (2G‐TKIs) while in sustained deep molecular response. Patients were divided among those who maintained response (group 1, n = 123) and those who lost major molecular response (group 2, n = 45). Mean BCR::ABL1 RNA levels 1 month after discontinuation were higher in group 2 than in group 1 (p = 0.0005) and the difference was more evident 2 months after stop (p < 0.0001). The same trend was found both for imatinib and 2G‐TKIs. A receiver operating characteristic (ROC) analysis to determine a threshold value of BCR::ABL1 at 1 month after discontinuation identified a cut‐off value of 0.0051%, with 92.2% specificity, 31.7% sensitivity and a likelihood ratio of 4.087.
Patient‐reported outcomes in childhood head and neck rhabdomyosarcoma survivors and their relation to physician‐graded adverse events—A multicenter study using the FACE‐Q Craniofacial module
Authors: Morfouace Michèle , Hol Marinka L. F. , Schoot Reineke A. , Slater Olga , Indelicato Daniel J. , Kolb Frédéric , Smeele Ludwig E. , Merks Johannes H. M. , Rae Charlene , Maurice‐Stam Heleen , Klassen Anne F. , Grootenhuis Martha A. 
Published: October 2022

AbstractIntroductionAdverse events (AE) of treatment are prevalent and diverse in head and neck rhabdomyosarcoma (HNRMS) survivors. These AEs are often reported by physicians; however, patients' perceptions of specific AE are not well known. In this study, we explored patient‐reported outcomes measuring appearance, health‐related quality of life (HRQOL), and facial function in HNRMS survivors. Second, we assess the relationship between physician grading of AE and patient reporting.Materials and MethodsSurvivors of pediatric HNRMS, diagnosed between 1993 and 2017, who were at least 2 years after completing treatment were invited to an outpatient clinic as part of a multicenter cross‐sectional cohort study. At the outpatient clinics, survivors aged ≥8 years filled out the FACE‐Q Craniofacial module; a patient‐reported outcome instrument measuring issues specific to patients with facial differences. AE were systematically assessed by a multidisciplinary team based on the Common Terminology Criteria of Adverse Events system.ResultsSeventy‐seven survivors with a median age of 16 years (range 8–43) and median follow‐up of 10 years (range 2–42) completed the questionnaire and were screened for AEs. Patient‐reported outcomes varied widely between survivors. Many survivors reported negative consequences: 82% on appearance items, 81% on HRQOL items, and 38% on facial function items. There was a weak correlation between physician‐scored AEs and the majority of patient‐reported outcomes specific for those AEs.ConclusionsPhysician‐graded AEs are not sufficient to provide tailored care for HNMRS survivors. Findings from this study highlight the importance of incorporating patient‐reported outcome measures in survivorship follow‐up.
Identifying HER2 from serum‐derived exosomes in advanced gastric cancer as a promising biomarker for assessing tissue HER2 status and predicting the efficacy of trastuzumab‐based therapy
Authors: Li Qian , Lv Minzhi , Lv Lihua , Cao Nida , Zhao Aiguang , Chen Jiayan , Tang Xi , Luo Rongkui , Yu Shan , Zhou Yan , Cui Yuehong , Guo Wei , Liu Tianshu 
Published: October 2022

AbstractPurposeThis study aimed to evaluate the clinical relevance of exosomal HER2 (Exo HER2) level in assessing the tissue HER2 status and predicting the efficacy of trastuzumab treatment.MethodsIn this prospective study, patients with advanced gastric cancer (AGC) from three hospitals between August 2016 to November 2020 were enrolled. The Exo HER2 level was detected by enzyme‐linked immunosorbent assay. Receiver operating characteristic curve (ROC) was drawn referring to the HER2 tissue status to assess the diagnostic value of Exo HER2. Cox proportional hazards regression and logistic regression were used to evaluate the association between Exo HER2 and progression‐free survival (PFS), overall survival (OS), and objective response rate (ORR) in patients who received trastuzumab‐based first‐line therapy.ResultsIn this study, 242 patients with advanced or metastatic gastric adenocarcinoma were registered. Of these, 238 AGC patients were eligible for evaluating serum‐derived exosome HER2 diagnostic value, including 114 HER2‐positive. Finally, 64 were eligible for efficacy analysis. The area under the ROC curve was 0.746. The optimal cutoff value for diagnosing tissue HER2‐positive status was 729.95 ng/ml, with a sensitivity of 66.7% and a specificity of 74.2%. In 64 patients treated with trastuzumab, higher baseline Exo HER2 level indicated better prognosis. 844 ng/ml and 723 ng/ml were the right cutoffs for distinguishing the population with superior PFS (hazard ratio [HR] = 0.41, P = 0.017) and OS (HR = 0.30, P < 0.001), respectively.ConclusionSerum exosomal HER2 level might serve as an effective biomarker for assessing tissue HER2 status in AGC and screening the potential patients who might benefit from anti‐HER2 therapy.
Systemic immune changes accompany combination treatment with immunotoxin LMB‐100 and nab‐paclitaxel
Authors: Pegna Guillaume Joe , Lee Min‐Jung , Peer Cody J. , Ahmad Mehwish I. , Venzon David J. , Yu Yunkai , Yuno Akira , Steinberg Seth M. , Cao Liang , Figg William D. , Donahue Renee N. , Hassan Raffit , Pastan Ira , Trepel Jane B. , Alewine Christine 
Published: October 2022

AbstractLMB‐100 is a novel immune‐conjugate (immunotoxin) that targets mesothelin. A phase 1/2 clinical trial was conducted (NCT02810418) with primary objectives assessing the safety and efficacy of LMB‐100 ± nab‐paclitaxel. Participant blood samples were analyzed for changes in serum cytokines and circulating immune cell subsets associated with response or toxicity. On Arm A, participants (n = 20) received standard 30‐minute LMB‐100 infusion with nab‐paclitaxel. Although clinical efficacy was observed, the combination caused intolerable capillary leak syndrome (CLS), a major toxicity of unclear etiology that affects many immunotoxin drugs. Participants developing CLS experienced rapid elevations in IFNγ and IL‐8 compared to those without significant CLS, along with midcycle increases in Ki‐67‐ CD4 T cells that were CD38, HLA‐DR, or TIM3 positive. Additionally, a strong increase in activated CD4 and CD8 T cells and a concurrent decrease in Tregs were seen in the single Arm A patient achieving a partial response. In Arm B, administration of single agent LMB‐100 to participants (n = 20) as a long infusion given over 24–48 h was investigated based on pre‐clinical data that this format could reduce CLS. An optimal dose and schedule of long infusion LMB‐100 were identified, but no clinical efficacy was observed even in patients receiving LMB‐100 in combination with nab‐paclitaxel. Despite this, both Arm A and B participants experienced increases in specific subsets of proliferating CD4 and CD8 T cells following Cycle 1 treatment. In summary, LMB‐100 treatment causes systemic immune activation. Inflammatory and immune changes that accompany drug associated CLS were characterized for the first time.
LncRNA SNHG3 enhances BMI1 mRNA stability by binding and regulating c‐MYC: Implications for the carcinogenic role of SNHG3 in bladder cancer
Authors: Xie Jinbo , Ni Jinliang , Shi Huajuan , Wang Keyi , Ma Xiaoying , Li Wei , Peng Bo 
Published: October 2022

AbstractThe transformation of nonmuscle‐invasive bladder cancer (BLCa) to muscle‐invasive type and distant metastasis are the two major threats to patients after surgery. Thus, it is important to identify the key genes of BLCa cell invasion and metastasis. Long noncoding RNA (lncRNA) is a potential clinical tool for cancer diagnosis and treatment. Herein, we verified that lncRNA SNHG3 is upregulated in human BLCa specimens and is proportional to poor clinical prognosis via a combination of bioinformatic analyses and wet bench experiments. Then, we constructed SNHG3 knockdown and overexpression cell models via lentiviral packaging and CRISPR‐Cas9 technique. Fluorescence in situ hybridization assay showed that SNHG3 is distributed in both the nucleus and cytoplasm of BLCa cell lines. In vitro assays including CCK‐8, EdU, colony formation, wound healing, transwell, and tube formation demonstrated that SNHG3 knockdown and overexpression potently inhibited and enhanced BLCa cell proliferation, migration, invasion, and angiogenesis. In addition, IVIS imaging revealed that SNHG3 knockdown could significantly inhibit M‐NSG mice xenograft tumor growth. Next, RNA sequencing, bioinformatics analyses and western blots indicated that SNHG3 could promote c‐MYC expression. RNA immunoprecipitation, actinomycin D assay and western blot assays suggested that SNHG3 could also bind c‐MYC protein which subsequently facilitate the stabilization of BMI1 mRNA, thus enhancing BMI1 protein level. However, SNHG3 knockdown had a slightly weaker inhibitory effect on BMI1 expression than c‐MYC knockdown. Further, in vitro assays demonstrated that BMI1 knockdown could suppress the SNHG3 activation‐induced tumor promoting effect in BLCa cells. Overall, this study has provided new insights into the potential implication of lncRNA SNHG3 in the pathogenesis of BLCa. Importantly, SNHG3/c‐MYC/BMI1 axis may be a novel target for regulating tumor growth and metastasis in BLCa patients.
Attention‐Deficit/hyperactivity disorder and centralized pain: A review of the case of John F. Kennedy
Authors: Kasahara Satoshi , Matsudaira Ko , Sato Naoko , Niwa Shin‐Ichi 
Published: October 2022

AbstractJohn Fitzgerald Kennedy (JFK), the 35th President of the USA, had chronic low back pain deemed to be centralized pain. Reportedly, attention‐deficit/hyperactivity disorder (ADHD) could associate with centralized pain. Based on his biographies, JFK could have had ADHD, a plausible cause of pain that afflicted him.
An unusual presentation of celiac disease in adult patient
Authors: Frioui Refka , Zaouak Anissa , Chamli Amal , Jouini Raja , Fenniche Samy , Hammami Houda 
Published: October 2022

AbstractPyoderma gangrenosum is among the exceptional extra‐intestinal manifestations of celiac disease. We report a case of a 52‐year‐old patient who presented with pyoderma gangrenosum that turned out to be the initial presentation of celiac disease.
Preliminary results from the LUX‐Dx insertable cardiac monitor remote programming and performance (LUX‐Dx PERFORM) study
Authors: Stolen Craig , Rosman Jonathan , Manyam Harish , Kwan Brian , Kelly Jonathan , Perschbacher David , Garner John , Richards Mark 
Published: October 2022

AbstractDespite the wide adoption of insertable cardiac monitors (ICMs), high false‐positive rates, suboptimal signal quality, limited ability to detect atrial flutter, and lack of remote programming remain challenging. The LUX‐Dx PERFORM study was designed to evaluate novel technologies engineered to address these issues. Here, we present preliminary results from the trial focusing on the safety of ICM insertion, remote monitoring rates, and the feasibility of remote programming. LUX‐Dx PERFORM is a multicenter, prospective, single‐arm, post‐market, observational study with planned enrollment of up to 827 patients from 35 sites in North America. A preliminary cohort consisting of the first 369 patients who were enrolled between March and October 2021 was selected for analysis. Three hundred sixty‐three (363) patients had ICM insertions across inpatient and outpatient settings. The mean time followed was 103.4 ± 61.8 days per patient. The total infection rate was 0.8% (3/363). Interim results show high levels of remote monitoring with a median 94% of days with data transmission (interquartile range: 82–99). Thirteen (13) in‐clinic and 24 remote programming sessions were reported in 34 subjects. Reprogramming examples are presented to highlight signal quality, the ability to detect atrial flutter, and the positive impact of remote programming on patient management. Interim results from LUX‐Dx PERFORM study demonstrate the safety of insertion, high data transmission rates, the ability to detect atrial flutter, and the feasibility of remote programming to optimize arrhythmia detection and improve clinical workflow. Future results from LUX‐Dx PERFORM will further characterize improvements in signal quality and arrhythmia detection.
N‐terminal pro‐B‐type natriuretic peptide and D‐dimer combined with left atrial diameter to predict the risk of ischemic stroke in nonvalvular atrial fibrillation
Authors: Shen Zican , Chen Dong , Cheng Hao , Tan Feng , Yan Jianwei , Deng Haiming , Fang Wei , Wang Sunan , Zhu Jianbing 
Published: October 2022

AbstractObjectivesWe aimed to explore the potential role of N‐terminal pro‐B‐type natriuretic peptide (NT‐proBNP), d‐dimer, and the echocardiographic parameter left atrial diameter (LAD) in identifying and predicting the occurrence of ischemic stroke (IS) in patients with nonvalvular atrial fibrillation (NVAF).MethodsWe conducted a retrospective study of 445 patients with NVAF in the First Affiliated Hospital of Nanchang University. They were divided into the NVAF (309 cases) and NVAF with stroke (136 cases) groups according to whether acute ischemic stroke (AIS) occurred at admission. Multivariate logistic regression was used to analyze the odds ratio (OR) of NT‐proBNP, d‐dimer, and LAD for IS. The predictive value of NT‐proBNP,
d‐dimer, and LAD in identifying the occurrence of IS in NVAF was determined by plotting the receiver operating characteristic (ROC) curves.ResultsNT‐proBNP, d‐dimer, and LAD levels were significantly higher in the NVAF with stroke group than in the NVAF group (p < .05). NT‐ProBNP,
d‐dimer, and LAD were independently associated with IS in NVAF patients (odds ratio [OR] = 1.12, 95% confidence interval [CI]: 1.08–1.16; OR = 1.87, 95% CI: 1.37–2.55; OR = 1.21, 95% CI: 1.13–1.28, p < .01). The optimal cutoff points for NT‐ProBNP,
d‐dimer, and LAD levels to distinguish the NVAF group from the NVAF with stroke group were 715.0 pg/ml, 0.515 ng/ml, and 38.5 mm, respectively, with the area under the curve (AUC) being [0.801 (95% CI: 0.76–0.84); 0.770 (95% CI: 0.72–0.85); 0.752 (95% CI: 0.71–0.80), p < .01]. The combined score of NT‐proBNP,
d‐dimer, and LAD improved the predictive efficacy of the single index, with an AUC of 0.846 (95% CI: 0.81–0.88, p < .01), sensitivity of 77.2%, and specificity of 76.4%.ConclusionNT‐proBNP, d‐dimer, and the echocardiographic parameter LAD have outstanding value in predicting the risk of IS in patients with NVAF.
Pharmaceutical drugs expiry date tracking: A visionary approach
Authors: Goyal Pankhuri , Goyal Nupur , Singh Pavneet , Mittal Nischay , Jindal Neeru , Kaur Kanwarpreet 
Published: October 2022

SummaryEvery drug manufacturer is legally bound to display the expiry date on all pharmaceutical products as medicines may not be safe or as effective after the expiry date. The problem is that any pharmaceutical drug has all the important information such as expiry date, batch no., manufacturing date written on a small corner of the packaging. Once that part is rubbed or torn out while taking a pill from the leaflet or otherwise, there is no way to know that crucial information making that whole drug a complete waste as one cannot take risks with their health. This article proposes a solution to find out this crucial information even after it is lost from the leaflet. Therefore, this research work is focused to design an application (app) based on the features of optical character recognition, database management system, auto‐classification, aiming at helping people to use it with ease. One just need to scan the medicine once and the app will autosave all the required credentials that can be accessed later on by scanning it again. Compared to the existing technologies, this app does not depend on QR code as it can also be torn out while taking any pill.
An efficient early detection of diabetic retinopathy using dwarf mongoose optimization based deep belief network
Authors: Abirami A. , Kavitha R. 
Published: October 2022

SummaryIn general, diabetic retinopathy (DR) is a common ocular disease that causes damage to the retina due to blood leakage from the vessels. Earlier detection of DR becomes a complicated task and it is necessary to prevent complete blindness. Various physical examinations are employed in DR detection but manual diagnosis results in misclassification results. Therefore, this article proposes a novel technique to predict and classify the DR disease effectively. The significant objective of the proposed approach involves the effective classification of fundus retinal images into two namely, normal (absence of DR) and abnormal (presence of DR). The proposed DR detection utilizes three vital phases namely, the data preprocessing, image augmentation, feature extraction, and classification. Initially, the image preprocessing is done to remove unwanted noises and to enhance images. Then, the preprocessed image is augmented to enhance the size and quality of the training images. This article proposes a novel modified Gaussian convolutional deep belief network based dwarf mongoose optimization algorithm for effective extraction and classification of retinal images. In this article, an ODIR‐2019 dataset is employed in detecting and classifying DR disease. Finally, the experimentation is carried out and the proposed approach achieved 97% of accuracy. This implies that our proposed approach effectively classifies the fundus retinal images.
A comparison of nest‐site characteristics for two sympatric Estrildid finches (Uraeginthus spp.) in Tanzania
Authors: Ota Nao 
Published: October 2022

AbstractIt is well known that birds have great diversity in nesting strategies, but we still have limited knowledge of the variation among species that share the habitat. Here, I will report and compare the nesting strategies between the two sympatric songbirds. Blue‐capped (Uraeginthus cyanocephalus) and red‐cheeked cordon‐bleus (Uraeginthus bengalus) are socially monogamous, biparental songbirds (family Estrildidae) that sympatrically inhabit arid landscapes with trees and bushes in East Africa. Both species build domed nests with grasses that are often located near wasp nests. They also sometimes take over old weaver (family Ploceidae) nests. While these nesting strategies are already described as common behavioral traits in both species in the literature, interspecies variation in these nesting strategies in areas of sympatry is not reported. My initial field observation during their breeding season suggested that whether these nesting strategies were adopted or not varied somewhat between the sympatric cordon‐bleus. Thus, I carried out a more formal investigation to test these differences. I found that red‐cheeked cordon‐bleus built their nests near wasp nests more frequently than blue‐capped cordon‐bleus, while I did not find any other significant differences between the nesting sites of the two species, such as the use of weaver nests, the types of nesting plants, or nest heights. These results suggest that the sympatric cordon‐bleus share several nest‐site characteristics, but that red‐cheeked cordon‐bleus have an affinity for nesting near wasp nests. Further studies will be required to elucidate the costs and benefits of these nesting strategies or the role that adjacency to wasp nests might play in the sympatry of the two species.