bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ocular surface squamous neoplasia with 360° limbal involvement: A comparative study of 130 patients
Authors: Vempuluru Vijitha S , Ghose Neha , Pattnaik Monalisha , Mohamed Ashik , Kaliki Swathi 
Published: March 2022

Abstract
Purpose
To describe the risk factors, clinical features and management outcomes of ocular surface squamous neoplasia (OSSN) with 360° of limbal involvement (360-OSSN).
Methods
Retrospective comparative study.
Results
Of 1250 patients diagnosed with OSSN during the study period, 30 (2%) had 360-OSSN. A total of 100 patients of OSSN with segmental limbal involvement (SL-OSSN) were included for comparison. 360-OSSN patients more often had longer duration of symptoms (mean, 17 months vs 8 months; p, 0.003), prior misdiagnosis (17% vs 6%, p, 0.13) and prior intervention (47% vs 13%; p, 0.0002) than patients with SL-OSSN. 360-OSSN tumors were more extensive (mean diameter, 24mm vs 8 mm; p < 0.0001), had diffuse ocular surface involvement (63% vs 3%, p < 0.0001), scleral fixity (57% vs 16%; p < 0.0001), corneal/scleral melt (17% vs 0%; p, 0.0005), intraocular tumor extension (17% vs 0%; p, 0.003), orbital tumor extension (33% vs 1%; p < 0.0001), and advanced T stage at presentation (Tis: 37% vs 76%, T1: 0% vs 15%; T2: 7% vs 4%; T3: 27% vs 4%; T4: 30% vs 1%; p < 0.001). Over a mean follow-up of 15 months in patients with 360-OSSN and 13 months in those with SL-OSSN, lymph node metastasis (8% vs 0%; p, 0.05) and distant metastasis (4% vs 0%; p, 0.23) was more common in 360-OSSN group vs SL-OSSN group.
Conclusion
Risk factors of 360-OSSN include prolonged symptoms, prior misdiagnosis and prior intervention. It represents an advanced form of disease with larger, diffuse lesions, propensity for corneo-scleral melt and invasive disease which requires aggressive management.
Molecularand Phylogenetic Characterization Of Cryptosporidium Species In The Saffron Finch Sicalis Flaveola
Authors: de Oliveira Francisco Carlos Rodrigues , Gallo Samira Salim Mello , Elizeu Taynara Kerolayne Santos , Ederli Nicole Brand 
Published: March 2022

Abstract
Cryptosporidium is the most common protozoan that can infect a wide variety of animals, including mammals and birds. Fecal samples of saffron finches, Sicalis flaveola, from a commercial establishment were screened for the presence of Cryptosporidium by the modified Ziehl–Neelsen technique and nested PCR of the 18S rRNA gene followed by sequencing of the amplified fragments. The species Cryptosporidium galli was identified in six saffron fiches, in addition to Cryptosporidium andersoni in one of the birds, indicating a mixed infection. Only two birds had feathers that were ruffled and dirty with feces. Concomitant infection with Isospora spp. was observed in all birds. In conclusion, this is the third report of parasitism by C. galli in S. flaveola and that this bird is a possible host of C. andersoni.
Light-dependence of Formate (C1) and Acetate (C2) Transport and Oxidation in Poplar Trees
Authors: Jardine Kolby J. , Lei Joseph , Som Suman , Souza Daisy , Clendinen Chaevien S. , Mehta Hardeep , Handakumbura Pubudu , Bill Markus , Young Robert P. , Brodie Eoin 
Published: March 2022

Abstract
BackgroundAlthough apparent light inhibition of leaf day respiration is a widespread reported phenomenon, the mechanisms involved, including utilization of alternate respiratory pathways and substrates and light inhibition of TCA cycle enzymes are under active investigation. Recently, acetate fermentation was highlighted as a key drought survival strategy mediated through protein acetylation and jasmonate signaling. Here, we evaluate the light-dependence of acetate transport and assimilation in Populus trichocarpa trees using a new dynamic xylem solution injection (DXSI) method for continuous studies of C1 and C2 organic acid transport and light-dependent metabolism. ResultsOver 7 days, 1.0 L of [13C]formate and [13C2]acetate solutions were delivered to the stem base of 2-year old potted poplar trees, while continuous diurnal observations were made in the canopy of CO2, H2O, and isoprene gas exchange together with δ13CO2. Stem base injection of 10 mM [13C2]acetate induced an overall pattern of canopy branch headspace 13CO2 enrichment (δ13CO2 +27‰) with a diurnal structure in δ13CO2 reaching a mid-day minimum following by a maximum shortly after darkening where δ13CO2 values rapidly increased up to +12‰. In contrast, 50 mM injections of [13C]formate were required to reach similar δ13CO2 enrichment levels in the canopy with δ13CO2 following diurnal patterns of transpiration. Illuminated leaves of detached poplar branches pretreated with 10 mM [13C2]acetate showed lower δ13CO2 (+20‰) compared to leaves treated with 10 mM [13C]formate (+320‰), the opposite pattern observed at the whole plant scale. Following dark/light cycles at the leaf-scale, rapid, strong, and reversible enhancements in headspace δ13CO2 by up to +60‰ were observed in [13C2]acetate treated leaves which showed enhanced dihydrojasmonic acid and TCA cycle intermediate concentrations. ConclusionsThe results are consistent with acetate in the transpiration stream as an effective activator of the jasmonate signaling pathway and respiratory substrate. The shorter lifetime of formate relative to acetate in the transpiration stream suggests rapid formate oxidation to CO2 during transport to the canopy. In contrast, acetate is efficiently transported to the canopy where an increased allocation towards mitochondrial dark respiration occurs at night. The results highlight the potential for an effective integration of acetate into glyoxylate and TCA cycles and the light-inhibition of citrate synthase as an important regulatory mechanism in controlling the diurnal allocation of acetate between anabolic and catabolic processes.
Endoscopic Trans-Mini-Cylinder Biopsy for Intraparenchymal Brain Lesions
Authors: Takeuchi Kazuhito , Ohka Fumiharu , Nagata Yuichi , Maeda Sachi , Tanahashi Kuniaki , Araki Yoshio , Yamamoto Taiki , Sasaki Hiroo , Mizuno Akihiro , Harada Hideyuki , Saito Ryuta 
Published: March 2022

Abstract
Purpose: Recently, the importance of molecular diagnosis has increased, along with the volume of specimens required for diagnosis. Biopsy procedures must simultaneously reduce invasiveness and ensure the collection of adequate tissue volume. We examined the efficacy and safety of endoscopic biopsy using a minimal diameter cylinder.Methods: Patients who underwent endoscopic biopsy surgery with a 6-mm diameter cylinder for intraparenchymal lesions were included. Postoperative hematoma formation and the extent of trajectory scarring were assessed. Molecular genetic analysis was performed for cases of diffuse infiltrated glioma.Results: Fifty-two procedures performed on 51 patients were analyzed in this study. Postoperative neurological deterioration was not observed in any case. A pathological diagnosis was made for all patients. Postoperative CT revealed no hematoma after 49 procedures and a small hematoma after 3 procedures, and no patients required additional treatment. A postoperative trajectory scar less than 5 mm in size was observed after 30 procedures, a scar of 5-10 mm was observed after 19 procedures, and a scar larger than 10 mm was observed after 3 procedures at 1 week after surgery, and 40, 6 and 0 scars were observed at 3 months after surgery. Molecular genetic analysis was performed in 17 cases, resulting in a change to a higher grade in the integrated diagnosis of 3 cases.Conclusions: Endoscopic biopsy using a small-diameter cylinder is a possible alternative biopsy technique for intraparenchymal lesions. This surgical technique is useful especially in cases where hemorrhagic complications are expected.Trial registrationRegistry name and number: Efficacy and safety of the neuro-endoscopic treatment for brain tumors. (2019-0254)
Exploring public interest from Twitter in 2021 using natural language processing for post-2020 biodiversity conservation strategies﻿
Authors: Ohtani Shimon 
Published: March 2022

Abstract

The need to develop more effective biodiversity conservation strategies for the post-2020 period is now being called for both globally and in individual countries. As an example of analysis that should serve as a clue for this purpose, this paper presents a methodology for exploring public interest in “biodiversity” by analyzing text posted on Twitter in 2021 using natural language processing, which is one of the big data analyses that have been rapidly advancing in recent years. First, the frequency of idioms was explored by aggregating bigrams in contexts where the word “biodiversity” is used, and then the tweet data set was classified into 40 meaningful topics and defined by LDA topic modeling. In addition, two ways of sentiment analysis by NRC emotion lexicon and VADER were used to visualize the rough emotional trends that can be read from the data set. And I then selected a topic on “Extinction of Species” for intensive discussion, and also picked up and discussed tweets about “30 by 30” as an example of a cross-topic analysis. However, developing measures to compensate for the weaknesses of incompleteness and non-representativeness of big data is also a major challenge. In the future, cultivating interdisciplinary knowledge and promoting collaboration among researchers in different fields and deep social networking literacy to enable a hybrid analysis of multiple data sets will be essential for effective biodiversity conservation strategies.
Intravesical Ectopic Prostatic Tissue May be Misdiagnosed as Nephrogenic Adenoma: a Case Report and Overview
Authors: Wu Yonglu , Wu Aiming , Li Zhiqin 
Published: March 2022

Abstract
Background Ectopic prostatic tissue is a relatively infrequent condition, usually found accidentally during imaging examinations or surgery. From 1967 to 2021, the reports of ectopic prostate tissue in English literature are increasing. It is rarely reported that ectopic prostate may be misdiagnosed as a nephrogenic adenoma (NA). Case presentationThis article reports a 45-year-old male patient with no symptoms. The computed tomography (CT) scan revealed a homogeneous isoechoic mass in the posterior inferior wall of the bladder. Firstly, the transurethral cystoscopic biopsy revealed a smooth sessile mass that covered normal bladder mucosa located in the middle of the interureteric ridge, and pathological results considered an intravesical NA. Then, the mass was completely resected under laparoscopic pneumovesicum and the pathological diagnosis was ectopic prostate tissue. Conclusions The clinical symptoms of ectopic prostate were similar to other bladder neoplasms, and fewer characteristics of imaging examinations are available to diagnose. Surgery was the mainstay treatment of ectopic prostatic tissue and has a good prognosis. Ectopic prostatic tissue presents as a tumor in the bladder, urologists may easily misdiagnose the condition like a tumor. Therefore, it is necessary to awareness of ectopic prostatic tissue to avoid misdiagnosis.
Variation of total anti-SARS-CoV-2 antibodies after primary BNT162b2 vaccination and homologous booster
Authors: Salvagno Gian Luca , Henry Brandon M. , Pighi Laura , De Nitto Simone , Lippi Giuseppe 
Published: March 2022

Abstract
Background: In this serosurveillance study, we investigated the variation of total anti-SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) antibodies in healthcare workers receiving primary BNT162b2 vaccination and homologous booster.Methods: A total number of 524 subjects (median age, 46 years; 65.3% females), were studied. All received primary BNT162b2 vaccination (two doses) and homologous booster (one dose) >8 months after completing the primary cycle. Blood samples were collected before the first and second vaccine doses, at 1, 3 and 6 months after the second dose, as well as before and 1 month after booster. Total anti-SARS-CoV-2 neutralizing antibodies were assayed with Roche Elecsys Anti-SARS-CoV-2 S chemiluminescent immunoassay.Results: Overall, 65.1% subjects were baseline (i.e., prevaccination) SARS-CoV-2 seronegative and always tested SARS-CoV-2 negative (“N/N”), 16.2% were baseline SARS-CoV-2 seronegative but tested SARS-CoV-2 positive after receiving the vaccine booster dose (“N/P”), whilst 18.7% were baseline SARS-CoV-2 seropositive and always tested SARS-CoV-2 negative afterwards (“P/N”). All groups displayed a similar trend of total anti-SARS-CoV-2 S antibodies throughout the study period, though the P/N cohort exhibited higher values compared to the other two groups until receiving the booster, after which the levels become similar in all cohorts. Significant differences in total anti-SARS-CoV-2 S antibodies values were not found between N/N and N/P groups, neither 1 month after booster. The rate of subjects with protective antibodies values become 100% in all groups after booster.Conclusions: Although baseline seropositivity is associated with more pronounced humoral immune response following primary vaccination compared to never infected subjects, SARS-CoV-2 infection after booster does not significantly foster antibody titers.
Comparative Effectiveness of Two Behavioral Change Intervention Packages for Tobacco Cessation Initiated in The Tertiary Care Setting of North India-Protocol for A Two-Arm Randomized Controlled Trial
Authors: Dhawan Priyanka , Goel Sonu , Aggarwal Ashutosh , Ghosh Abhishek , Vijayvergiya Rajesh , Medhi Bikash , Khurana Dheeraj , Verma Roshan 
Published: March 2022

Abstract
ackground:To reduce the global burden of tobacco use, clinical guidelines support behavioral therapy and pharmacotherapy as preferred interventions for tobacco cessation. The evidence based behavioral interventions has consistently shown to be impactful in community settings, however its efficacy has not been established in hospital settings. The current study aims to investigate impact of trans-theoretical based behavioral intervention package on tobacco users suffering from non-communicable diseases attending tertiary care settings of North India.Methods/Design: A two arm randomized controlled trial (RCT) in a tertiary health care hospital will be performed. A total of 360 tobacco users attending NCD clinics in four departments: Cardiology, Neurology, Pulmonary Medicine, ENT (Otolaryngology) will be recruited over a period of three months. After ascertaining the eligibility criteria they will be followed up to 6 months (1, 3, 6) for their tobacco use status, readiness to quit, nicotine dependence, stage of behavior change and self-reported & biochemical validation (urine cotinine) for tobacco abstinence. Assignment of intervention including allocation concealment, sequence generation, blinding will be done as per SPIRIT guidelines for RCT protocols.Discussion:As no strong evidence exists about the effectiveness of tobacco cessation intervention in tertiary settings, the current study will build evidence about the similar interventions in such settings.Trial Registration: CTRI/2019/09/021406
Effects of pre-workout multi-ingredient supplement on anaerobic performance: randomized double-blind crossover study
Authors: Kaczka Piotr , Batra Amit , Kubicka Katarzyna , Maciejczyk Marcin , Rzeszutko-Bełzowska Agata , Pezdan-Śliż Iwona , Michałowska-Sawczyn Monika , Przydział Marta , Płonka Artur , Cięszczyk Paweł , Humińska-Lisowska Kinga , Zając Tomasz 
Published: March 2022

Abstract

Background: The purpose of this study was to investigate the acute effects of commercially available pre-workout supplement on anaerobic performance in resistance trained men.Methods: Twenty-three men underwent three testing sessions administrated in a randomized and double-blind fashion separated by a seven-day break. The participants performed three exercise tests: isokinetic strength test, maximal strength test and Wingate test. Statistical analysis was conducted in R environment. Linear mixed models were estimated via R package lme4.Results: The mean knee peak torque was significantly greater in supplemented group for right and left knee flexors (placebo: 103.17 ± 37.61 Nm, and supplemented group: 131.84 ± 29.31 Nm where p=0.001, and placebo: 103.72 ± 39.35, and supplemented group: 129.38 ± 28.44, where p=0.001; respectively) as well as for right and left knee extensors (placebo: 202.65 ± 58.64 Nm, and supplemented group: 237.22 ± 54.75 Nm where p=0.001, and placebo: 203.27 ± 63.2 Nm versus supplemented group: 229.84 ± 50.8 Nm where p=0.002; respectively).The significant difference was observed in mean anaerobic power between supplemented and placebo group for right and left knee flexors (p=0.002 and p=0.005, respectively) as well as for right and left knee extensors (p=0.001 and p=0.002; respectively).There was also observed that the time to peak torque was significantly greater in supplemented group for right and left knee flexors (p=0.002 for both legs). The significant difference was also observed in mean power between supplemented and placebo group during Wingate test (placebo: 8.49 ± 0.57 W/kg, and supplemented group: 8.66 ± 0.55 W/kg where p=0.038). Moreover the mean 3-RM strength test was significantly greater in supplemented group with p=0.001.Conclusions: The results of the study indicate that the supplement significantly improves upper and lower body strength and power output in resistance trained men.
Solving Full NxNxN Rubik’s Supercube Using Genetic Algorithm
Authors: Świta Robert , Suszyński Zbigniew 
Published: March 2022

Abstract
This article presents an algorithm using an evolutionary approach to the problem of solving a full Rubik's NxNxN supercube i.e. orienting all cubies, including internal ones, not only by their face colors, but to the same orientation in 3D space. The problem is formally defined by a matrix representation using affine transformations of cubies. The solving strategy consists of series of genetic algorithms which try to find better cube configuration from the current one. After finding one, moves are executed, changing current configuration. This strategy is repeated until solving the cube. Genetic algorithm restricts movements to the current cluster, solving the cube in stages from the center of the cube to the outside. Movements which solve clusters are saved in the form of macros and used for training and acceleration of the algorithm. The goal of the algorithm is to minimize solving time, not the count of moves.
PBK is a potential therapeutic target and prognostic biomarker in ovarian cancer: integrating bioinformatics and clinical analysis
Authors: Lin Lina , Ge Yingying , Nong Weixia , Zeng Xia , Liu Chang , Wu Song , Wei Xing , Luo Bin , Zhang Qingmei , xie xiaoxun 
Published: March 2022

Abstract
Purpose Ovarian cancer (OC) remains the most lethal gynecological malignancy worldwide. The recrudescence and chemoresistance are main reasons for the high mortality of OC. Identifying effective therapeutic targets is an urgent need. Cancer testis antigens (CTAs) are attractive targets for cancer therapy because of their expression restriction. Methods Based on the RNAseq data of OC in The Cancer Genome Atlas (TCGA) database, we screened the differentially expressed genes of OC from the Cancer-Testis Database and created a protein–protein interaction (PPI) network to analyze co-expressed genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to explore the potential functions of genes. The 3D structure of the protein was simulated and domain interactions were analyzed. We evaluated the expression of candidate gene in OC via qRT-PCR and immunohistochemistry, its correlation with clinical indicators was also analyzed. Results Seven significant CTAs were found to be differentially expressed in OC, PBK was eventually selected as a prognostic factor. The established PPI network contained 93 co-expression genes with 966 protein interactions. Functional enrichment analysis showed that PBK was involved in regulating cell cycle, cell division and mitosis. PBK shares common domains with CDK1, CDK2, PLK1, CCNA2 and CCNB1. PBK was apparently up-regulated in OC, and its overexpression was correlated with the FIGO stage, lymph-node metastasis, and chemoresistance. Conclusion PBK was highly expressed in OC and related to poor prognosis, it functions as a crucial cell-cycle regulator. PBK could be a potential biomarker for OC therapies and prognosis prediction.
A review of climate change effects on practices for mitigating water quality impacts
Authors: Johnson Thomas , Butcher Jonathan , Santell Stephanie , Schwartz Sara , Julius Susan , LeDuc Stephen 
Published: March 2022

Abstract
Water quality practices are commonly implemented to reduce human impacts on land and water resources. In series or parallel in a landscape, systems of practices can reduce local and downstream pollution delivery. Many practices function via physical, chemical, and biological processes that are dependent on weather and climate. Climate change will alter the function of many such systems, though effects will vary in different hydroclimatic and watershed settings. Reducing the risk of impacts will require risk-based, adaptive planning. Here, we review the literature addressing climate change effects on practices commonly used to mitigate the water quality impacts of urban stormwater, agriculture, and forestry. Information from the general literature review is used to make qualitative inferences about the resilience of different types of practices. We discuss resilience in the context of two factors: the sensitivity of practice function to changes in climatic drivers, and the adaptability, or relative ease with which a practice can be modified as change occurs. While only a first step in addressing a complex topic, our aim is to help communities incorporate consideration of resilience to climate change as an additional factor in decisions about water quality practices to meet long-term goals.
Composite membrane of polyguanidine cationic surface for desalination
Authors: Han Qingfeng , Liu Dongqing , Huang Xiaohua , Xie Qinxing , Meng Jianqiang 
Published: March 2022

Abstract
Surface positve-charged composite membranes were prepared through two guanidine group containing polymers, polyhexamethylene guanidine (PHMG) and polyhexamethylene biguanidine (PHMB). They were used as aqueous phase monomers in an interfacial polymerization (IP) process reacting with trimesoyl chloride (TMC) on the surface of polysulfone (Psf) ultrafiltration membrane. Piperazine (PIP) provided the best pH adjustment among the chosen regulators. As guanidine groups dissociate in water and bring ammonium cations to membrane surfaces, both polyguanidine endowed membranes reacted with divalent metal cations better compared with Na+ and Li+. The rejection rates were more than 90% for all chosen divalent metal salts. PHMG membranes displayed excellent enrichment of Li+ from a mixed solution of Mg2+ and Li+ salts. The Mg2+/Li+ mass ratio decreased from 60 to 3.7, accompanying a −3.6% rejection to Li+. Although partial guanidine groups participated in the IP reaction, bactericidal rates of membranes were both higher than 99% in Gram-negative E. coli and Gram-positive S. aureus tests.