bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying the unmet supportive care needs of individuals affected by testicular cancer: a systematic review
Authors: Doyle R. , Craft P. , Turner M. , Paterson C. 
Published: July 2022

Abstract

Purpose
To critically appraise studies to identify experiences of unmet supportive care needs of individuals affected by testicular cancer.

Methods
A registered priori systematic review was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. CINAHL, PsycINFO, and MEDLINE were searched for quantitative, qualitative, and mixed methods studies using a wide range of search terms. All articles were double screened according to a pre-determined eligibility criterion. Reference lists of the final included studies were checked for further eligible studies. The review process was managed using Covidence systematic review software. Data from the studies were extracted, methodological quality appraisal conducted, and a narrative synthesis conducted.

Results
Of the 72 papers identified, 36 studies were included. In descending order of frequency of need, psychological needs were identified in 26/36, physical needs 18/36, interpersonal/intimacy needs 19/36, health system/information needs 11/36, cognitive needs 9/36, social needs 7/36, and of equal frequencies counts of 4/36 for family, practical, and patient-clinician information needs. Only one study explored spiritual needs and no daily living needs were identified.

Conclusions
The experience of needs varied in terms of frequency and distress which were commonly influenced by the age of the individual across the cancer care continuum persisting after 1-year post-treatment.

Implications for Cancer Survivors
When caring for individuals affected by testicular cancer, clinicians are encouraged to take a holistic lens to cancer care, particularly to explore issue or concerns that young men affected by testicular cancer might be embarrassed or reticent to discuss.
Genetic Testing in Acute and Chronic Pancreatitis
Authors: Piseddu Ignazio , Vielhauer Jakob , Mayerle Julia 
Published: July 2022

Abstract
Purpose of review
Premature intracellular activation of pancreatic zymogens leads to the initiation of pancreatitis, which in up to 25% leads to chronic tissue destruction, exocrine and endocrine organ failure, and a moderate increased risk of pancreatic cancer development. Whereas in many cases, the trigger of organ damage is identified, diagnostic workup in a significant number of patients does not reveal the underlying etiology of pancreatic inflammation. In these cases, alterations in different pancreatic susceptibility genes have been described to be directly or indirectly involved in disease development. In this review, we want to give an update on the most important pancreatitis risk genes and their impact on clinical diagnostics and risk stratification as well as possible treatment options.

Recent findings
Genetic testing is not routinely implemented in the diagnostic workup of acute or chronic pancreatitis, as most genetic variations are not considered causative for pancreatitis development but confer increased susceptibility and genetic testing rarely changes disease management. However, in patients with recurrent pancreatitis episodes of unknown etiology after intensive diagnostic work-up, in patients with a family history of pancreatitis, relatives of patients with hereditary pancreatitis, and patients with disease onset at young age, genetic testing and counseling is recommended. Besides well-established susceptibility genes such as PRSS1, SPINK1, CPA1, and CFTR, additional genes such as TRPV6 and rare genetic alterations in established risk genes have been recently identified which significantly contribute to the risk of pancreatitis, involving different molecular mechanisms.

Summary
When genetic testing is considered, we propose screening at least for PRSS1, SPINK1, CPA1, and CFTR gene variants. The emergence of next-generation sequencing methods could also render larger gene panels possible and clinically meaningful to detect rare variants with high-risk phenotypes. Here we summarize, evaluate, and convey in the form of practical recommendations the current level of knowledge with respect to definition, etiology, and genetic diagnostics of all forms of inherited pancreatitis.
Tbr1 Misexpression Alters Neuronal Development in the Cerebral Cortex
Authors: Crespo Inmaculada , Pignatelli Jaime , Kinare Veena , Méndez-Gómez Héctor R. , Esgleas Miriam , Román María José , Canals Josep M. , Tole Shubha , Vicario Carlos 
Published: July 2022

Abstract Changes in the transcription factor (TF) expression are critical for brain development, and they may also underlie neurodevelopmental disorders. Indeed, T-box brain1 (Tbr1) is a TF crucial for the formation of neocortical layer VI, and mutations and microdeletions in that gene are associated with malformations in the human cerebral cortex, alterations that accompany autism spectrum disorder (ASD). Interestingly, Tbr1 upregulation has also been related to the occurrence of ASD-like symptoms, although limited studies have addressed the effect of increased Tbr1 levels during neocortical development. Here, we analysed the impact of Tbr1 misexpression in mouse neural progenitor cells (NPCs) at embryonic day 14.5 (E14.5), when they mainly generate neuronal layers II–IV. By E18.5, cells accumulated in the intermediate zone and in the deep cortical layers, whereas they became less abundant in the upper cortical layers. In accordance with this, the proportion of Sox5+ cells in layers V–VI increased, while that of Cux1+ cells in layers II–IV decreased. On postnatal day 7, fewer defects in migration were evident, although a higher proportion of Sox5+ cells were seen in the upper and deep layers. The abnormal neuronal migration could be partially due to the altered multipolar-bipolar neuron morphologies induced by Tbr1 misexpression, which also reduced dendrite growth and branching, and disrupted the corpus callosum. Our results indicate that Tbr1 misexpression in cortical NPCs delays or disrupts neuronal migration, neuronal specification, dendrite development and the formation of the callosal tract. Hence, genetic changes that provoke ectopic Tbr1 upregulation during development could provoke cortical brain malformations.
Microstratigraphic analysis of the main Roman road in Hispania: the Via Augusta where it passes through the Ianus Augustus (Mengíbar, Spain)
Authors: Gutiérrez-Rodríguez Mario , Lechuga Chica Miguel Ángel , Moreno Padilla María Isabel , Bellón Ruiz Juan Pedro 
Published: July 2022

AbstractRoman roads are omnipresent in the Mediterranean basin. Despite the methodological advances achieved, interdisciplinary studies including geoarchaeological techniques are still rare. The aim of this study is to offer a microstratigraphic analysis of an important Roman road in order to characterise the raw materials and construction techniques used to build it and their evolution over time. Our research focuses on theViaAugusta, the longest road in RomanHispania, where it passes through theIanus Augustus(Mengíbar, Spain), a monumental complex on the frontier between the provinces ofBaeticaandTarraconensis. Archaeological excavations of this road have revealed vertical stratigraphic variations, suggesting the presence of several transit surfaces and repair works. A protocol was designed to characterise the road deposits at the microscale, where micromorphology revealed six overlying roads and their construction techniques. The combination of micromorphology, µ-XRF and the study of the textural parameters of the deposits allowed us to identify the raw materials used and their sources, as well as the specific features generated during the construction and use of the road. The results show how the technical solutions used survived as a tradition for centuries, and how the repair works identified in the stratigraphic sequence have a correlation with the road maintenance works mentioned in the Roman epigraphic record ofHispania Baetica. This study shows how detailed microstratigraphic analyses of Roman roads are very effective in the characterisation of road biographies.
Pesticides Exposure-Induced Changes in Brain Metabolome: Implications in the Pathogenesis of Neurodegenerative Disorders
Authors: Rodrigues Joel Arvin , Narasimhamurthy Rekha K. , Joshi Manjunath B. , Dsouza Herman Sunil , Mumbrekar Kamalesh Dattaram 
Published: July 2022

AbstractPesticides have been used in agriculture, public health programs, and pharmaceuticals for many decades. Though pesticides primarily target pests by affecting their nervous system and causing other lethal effects, these chemical entities also exert toxic effects in inadvertently exposed humans through inhalation or ingestion. Mounting pieces of evidence from cellular, animal, and clinical studies indicate that pesticide-exposed models display metabolite alterations of pathways involved in neurodegenerative diseases. Hence, identifying common key metabolites/metabolic pathways between pesticide-induced metabolic reprogramming and neurodegenerative diseases is necessary to understand the etiology of pesticides in the rise of neurodegenerative disorders. The present review provides an overview of specific metabolic pathways, including tryptophan metabolism, glutathione metabolism, dopamine metabolism, energy metabolism, mitochondrial dysfunction, fatty acids, and lipid metabolism that are specifically altered in response to pesticides. Furthermore, we discuss how these metabolite alterations are linked to the pathogenesis of neurodegenerative diseases and to identify novel biomarkers for targeted therapeutic approaches.
Recovery, Purification, Analysis and Chemical Modification of a Waste Cooking Oil
Authors: Azzena Ugo , Montenero Angelo , Carraro Massimo , Crisafulli Rosella , De Luca Lidia , Gaspa Silvia , Muzzu Andrea , Nuvoli Luca , Polese Riccardo , Pisano Luisa , Pintus Elisa , Pintus Salvatore , Girella Alessandro , Milanese Chiara 
Published: July 2022

AbstractDisposing of residual cooking oil is a major environmental concern, so its conversion into commercial products is a desirable goal. To design the chemical modification of a domestic waste oil into valuable biochemicals other than biodiesel, we analyzed a series of its samples, collected over a period of three years, using quantitative 1H-NMR. This analysis, allowing a quick determination of its main chemical characteristics, has rendered the image of a vegetable oil with an almost constant composition over time and with a relative low content of saturated fatty acids. To make this collection protocol more economical, we projected its chemical transformation into esters and epoxidized esters of long chain and branched alcohols, which could find employment as biolubricants and/or bioplasticizers. The one-pot transesterification of the waste cooking oil into esters of commercially available and biodegradable Guerbet alcohols was obtained under environmentally friendly conditions by employing commercial CaO as a catalyst. Additionally, microwave irradiation of this reaction allowed to optimize the energy expenditure by significantly reducing the reaction time as compared with reactions run under conventional heating. To improve their oxidation stabilities, the resulting esters were epoxidized under conditions useful to minimize side-reactions. An investigation of their main rheological properties shows that some of the resulting products display characteristics that make their use as biolubricants or bioplasticizers predictable. The entire process represents a virtuous example of circular economy.
Graphical Abstract
Composting the Invasive Toxic Seaweed Rugulopteryx okamurae Using Five Invertebrate Species, and a Mini-review on Composting Macroalgae
Authors: Patón D. , García-Gómez J. C. , Loring J. , Torres A. 
Published: July 2022

AbstractSince 2015, the invasive alga Rugulopteryx okamurae has explosively burst into the waters of the Strait of Gibraltar with serious repercussions on marine biodiversity, tourism and the fishing industry. Its elimination entails an enormous cost for the municipalities in the area and vermicomposting is proposed as a solution, but the anti-food secondary metabolites of the algae make it unfeasible. This work analyzed for the first time the bioremediation of this toxic algae using vermicomposting (Dendrobaena veneta and Eisenia fetida), blatticomposting (Eublaberus spp. “Ivory”), mealworms (Tenebrio molitor) and black soldier fly larvae (BSFL, Hermetia illucens). Both BSFL composting and blatticomposting are viable alternatives, as the toxics in the algae do not affect the long-term survival, growth or reproduction of these invertebrates. All other tested species do not resist consumption of R. okamurae. In parallel, a synthesis of current knowledge on marine macroalgae composting has been carried out. The results of this work will allow the use of insect farms in the upwelling areas that will eliminate algae, providing fertilizers and animal proteins that will be an economic aid to the municipalities in the affected coasts of southern Mediterranean Spain.
Graphical Abstract
Two novel qPCR assays to enhance black rail (Laterallus jamaicensis) eDNA surveys in the United States
Authors: Feist Sheena M. , Guan Xin , Malmfeldt Madeleine P. , Lance Richard F. 
Published: July 2022

AbstractBlack rails (Laterallus jamaicensis) are a rare and protected bird species. They are also notoriously difficult to monitor. eDNA surveys have proven beneficial for the detection of this elusive species, but additional developments are needed to enhance eDNA survey utility (including reliability of outcomes) in light of black rail status and associated high-profile conservation actions. The use of multiple assays functions to increase eDNA detection confidence by increasing the probability of amplification in low concentration, highly degraded samples (i.e., reducing the risk of false negatives) and by providing a multiplicative test for ruling out contamination as the source of qPCR amplification (i.e., reducing the risk of false positives). The two eDNA assays developed, optimized, and validated in this study will prove valuable for the continued surveillance of the secretive black rail, offering data to measure its distribution and occurrence over time and as influenced by anticipated climate-change-induced disturbances and/or habitat restoration/creation initiatives. The assays are applicable to both Eastern black rails and California black rails, and do not exhibit qPCR amplification in non-target, co-distributed taxa (including other Rallidae species) in the United States.
Consolidating understanding of variation as part of STEM: experimenting with plant growth
Authors: Watson Jane , Wright Suzie , Fitzallen Noleine , Kelly Ben 
Published: July 2022

AbstractIntegrated STEM activities are espoused as appropriate for enhancing student learning in relation to statistical concepts; however, a greater understanding of the way in which students’ ideas about those concepts develop is needed to maximise the learning potential offered by engagement in STEM activities. For this study, plant growth was chosen as a topic from the Year 6 Australian Science Curriculum as an appropriate context to employ aspects of the four STEM disciplines to explore students’ developing ideas about variation. Sixty-four Year 6 students across three school terms worked in groups of four to trial various treatments and their effects on the growth of radish or wheat seeds. This report considers two aspects of student learning related to this topic based on (i) the formative assessment of features of students’ workbook entries specifically related to variation during the part of the classroom activity based on their TinkerPlots graphs and (ii) the later summative evidence of learning in responses to end-of-year questions on the activity for 56 of the students. The workbook entries are presented via a qualitative analysis to provide evidence of the forming of understanding of variation in a STEM context, with the SOLO Taxonomy being employed to assess the longer-term evidence and developmental nature of that learning. Overall, a broader picture has emerged of the potential for developing appreciation of variation in a STEM context in primary school.
The multi-kinase inhibitor afatinib serves as a novel candidate for the treatment of human uveal melanoma
Authors: Shu Wenying , Zhu Xue , Wang Ke , Cherepanoff Svetlana , Conway R. Max , Madigan Michele C. , Zhu Hong , Zhu Ling , Murray Michael , Zhou Fanfan 
Published: July 2022

Abstract
Purpose
Uveal melanoma (UM) is the most common intraocular malignancy in adults with a poor prognosis and a high recurrence rate. Currently there is no effective treatment for UM. Multi-kinase inhibitors targeting dysregulated pro-tumorigenic signalling pathways have revolutionised anti-cancer treatment but, as yet, their efficacy in UM has not been established. Here, we identified the multi-kinase inhibitor afatinib as a highly effective agent that exerts anti-UM effects in in vitro, ex vivo and in vivo models.

Methods
We assessed the anti-cancer effects of afatinib using cell viability, cell death and cell cycle assays in in vitro and ex vivo UM models. The signaling pathways involved in the anti-UM effects of afatinib were evaluated by Western blotting. The in vivo activity of afatinib was evaluated in UM xenograft models using tumour mass measurement, PET scan, immunohistochemical staining and TUNEL assays.

Results
We found that afatinib reduced cell viability and activated apoptosis and cell cycle arrest in multiple established UM cell lines and in patient tumour-derived primary cell lines. Afatinib impaired cell migration and enhanced reproductive death in these UM cell models. Afatinib-induced cell death was accompanied by activation of STAT1 expression and downregulation of Bcl-xL and cyclin D1 expression, which control cell survival and cell cycle progression. Afatinib attenuated HER2-AKT/ERK/PI3K signalling in UM cell lines. Consistent with these observations, we found that afatinib suppressed tumour growth in UM xenografted mice.

Conclusion
Our data indicate that afatinib activates UM cell death and targets the HER2-mediated cascade, which modulates STAT1-Bcl-xL/cyclin D1 signalling. Thus, targeting HER2 with agents like afatinib may be a novel therapeutic strategy to treat UM and to prevent metastasis.
Insights into simultaneous adsorption and oxidation of antimonite [Sb(III)] by crawfish shell-derived biochar: spectroscopic investigation and theoretical calculations
Authors: Chen Hanbo , Gao Yurong , Li Jianhong , Sun Chenghua , Sarkar Binoy , Bhatnagar Amit , Bolan Nanthi , Yang Xing , Meng Jun , Liu Zhongzhen , Hou Hong , Wong Jonathan W.C. , Hou Deyi , Chen Wenfu , Wang Hailong 
Published: July 2022

Abstract
Removal of antimonite [Sb(III)] from the aquatic environment and reducing its biotoxicity is urgently needed to safeguard environmental and human health. Herein, crawfish shell-derived biochars (CSB), pyrolyzed at 350, 500, and 650°C, were used to remediate Sb(III) in aqueous solutions. The adsorption data best fitted to the pseudo-second-order kinetic and Langmuir isotherm models. Biochar produced at 350°C (CSB350) showed the highest adsorption capacity (27.7 mg g− 1), and the maximum 78% oxidative conversion of Sb(III) to Sb(V). The adsorption results complemented with infrared (FTIR), X-ray photoelectron (XPS), and near-edge X-ray absorption fine structure (NEXAFS) spectroscopy analyses indicated that the adsorption of Sb(III) on CSB involved electrostatic interaction, surface complexation with oxygen-containing functional groups (C = O, O = C–O), π–π coordination with aromatic C = C and C–H groups, and H-bonding with –OH group. Density functional theory calculations verified that surface complexation was the most dominant adsorption mechanism, whilst π–π coordination and H-bonding played a secondary role. Furthermore, electron spin resonance (ESR) and mediated electrochemical reduction/oxidation (MER/MEO) analyses confirmed that Sb(III) oxidation at the biochar surface was governed by persistent free radicals (PFRs) (•O2− and •OH) and the electron donating/accepting capacity (EDC/EAC) of biochar. The abundance of preferable surface functional groups, high concentration of PFRs, and high EDC conferred CSB350 the property of an optimal adsorbent/oxidant for Sb(III) removal from water. The encouraging results of this study call for future trials to apply suitable biochar for removing Sb(III) from wastewater at pilot scale and optimize the process.

Graphical abstract
Study of the isothermal bainitic transformation and austenite stability in an advanced Al-rich medium-Mn steel
Authors: Morawiec M. , Ruiz-Jimenez V. , Garcia-Mateo C. , Jimenez J. A. , Grajcar A. 
Published: July 2022

Abstract
Isothermal bainitic transformation of a lean medium-Mn steel containing (in mass%) 0.18C–3.6Mn–1.7Al–0.23Si–0.2Mo–0.04–Nb after full austenitization at 1100 ºC was studied by means of high resolution dilatometry. The effects of isothermal holding temperatures ranging from 450 to 350 °C on the bainitic transformation kinetics was studied experimentally characterizing the microstructure present after a holding time ranging from 15 min to 2 h. The obtained results showed that the bainitic transformation is uncompleted at temperatures above 425 °C. The carbon enrichment of the austenite during isothermal treatment at 450 °C and 425 °C is not enough to avoid martensitic transformation of the austenite during cooling to room temperature. Thus, it is obtained a mixed structure including bainitic ferrite and martensite. Decreasing the austempering temperature resulted in a more pronounced bainite formation. The kinetics of the transformation during austempering at 350ºC is quite similar to that observed at 400 °C, so that 60 min are needed in both cases to complete the reaction. However, local variations in chemical composition associated to segregation of Mn and Al during casting solidification results in differences in the transformation rate of bainitic reaction between different areas in the material. A balance difference between the dendritic and interdendritic areas is responsible for the differences observed among test samples in the kinetics of the isothermal bainitic transformation and the final microstructure.
Effective Variable Depth Local Search for the Budgeted Maximum Coverage Problem
Authors: Zhou Jianrong , Zheng Jiongzhi , He Kun 
Published: July 2022

AbstractWe address the Budgeted Maximum Coverage Problem (BMCP), which is a natural and more practical extension of the standard 0–1 knapsack problem and the set cover problem. Given m elements with nonnegative weights, n subsets of elements with nonnegative costs, and a total budget, BMCP aims to select some subsets such that the total cost of selected subsets does not exceed the budget, and the total weight of associated elements is maximized. In this paper, we propose a variable depth local search algorithm (VDLS) for the BMCP. VDLS first generates an initial solution by a greedy algorithm, then iteratively improves the solution through a partial depth-first search method, that can improve the solution by simultaneously changing the states (selected or not) of multiple subsets. Such method allows VDLS to explore the solution space widely and deeply, and to yield high-quality solutions. We further propose a neighbor structure to boost the algorithm performance, that is, both subsets have a neighbor relation if they share at least one common associated element. By applying the neighbor structure, VDLS can adjust the selected subsets while losing as few covered elements as possible. Since the existing BMCP benchmarks only have simple structures and small scales, we design 60 new instances with relatively large scales and complex structures to enrich the diversity of the BMCP instances. Experimental results on 30 public instances and 60 new instances we designed demonstrate that VDLS significantly outperforms the existing heuristic and the general CPLEX exact solver.