bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of Argonaute HIWI Gene in Colorectal Cancer Stem Cells and Colorectal Cancerous Tissue
Authors: Moeini Soheila , Marzouni Eisa Tahmasbpour , Harchegani Asghar Beigi , Ghatrehsamani Mahdi , Rahmanzadeh Saeid 
Published: January 2022

Abstract
Background: Recent studies have shown that Hiwi has a crucial role in stem cell self-renewal in various organisms and it is also associated with some cancers. In the present study, Hiwi expression was examined in different grades of primary human colorectal cancer (CRC), colorectal cancer stem cells (CRCSCs) and HT-29 CRC cell line. Methods and Results: CRC tissue samples were collected from 20 patients with CRC. Furthermore, HT-29 cell line, CRCSC and 13 normal colorectal tissue samples were prepared. Expression of Hiwi at mRNA and protein levels was determined by Real-time PCR, flow cytometry, Western blot and immunohistochemistry. Overexpression of Hiwi was detected in 40% (3.351 ± 2.94, P<0.05) of clinical CRC tissue specimens. We also observed a significant increase in the expression of Hiwi in CRCSCs (5.94 ± 0.05, P < 0.005). Hiwi expression in CRCSC was significantly higher compared to the colorectal cancer tissue and HT-29 cells (p<0.01). Hiwi mRNA level was significantly correlated to tumor grade (p < 0.01) and stage (p < 0.01).Conclusions: HiWi can be considered as an oncogene in progressive cancer and, therefore, it can be a valuable biomarker and target in therapeutic procedures.
Findings of virtual bronchoscopic navigation can predict the diagnostic rate of primary lung cancer by bronchoscopy in patients with peripheral lung lesions
Authors: Kitamura Atsushi , Tomishima Yutaka , Imai Ryosuke , Nishimura Naoki , Okafuji Kohei , Ro Shosei , Jinta Torahiko , Tamura Tomohide 
Published: January 2022

Abstract
Background and objective: Despite being highly invasive, bronchoscopy does not always obtain pathological specimens. Therefore, we investigated whether virtual bronchoscopic navigation (VBN) findings were associated with the diagnostic rate of primary lung cancer by bronchoscopy in patients with peripheral lung lesions.Methods: This study included patients with suspected malignant peripheral lung lesions who underwent bronchoscopy at our hospital from October 2013 to March 2020. Patients diagnosed with primary lung cancer were grouped according to whether their pathology could be diagnosed by bronchoscopy, and their clinical factors were compared. In addition, the distance between the edge of the lesion and the nearest branch (distance by VBN) was calculated. The distance by VBN and various clinical factors were compared with the diagnostic rates of primary lung cancer.Results: The study included 523 patients with 578 lesions. After excluding 55 patients who underwent multiple bronchoscopies, 381 patients were diagnosed with primary lung cancer. The diagnostic rate by bronchoscopy was 71.1% (271/381). Multivariate analysis revealed that the lesion diameter (odds ratio [OR] = 1.107), distance by VBN (OR = 0.94) and lesion structure (solid lesion or ground-glass nodule, OR = 2.988) influenced the risk of a lung cancer diagnosis. The area under the receiver operating characteristic curve for diagnosis based on the lesions diameter and distance by VBN was 0.810.Conclusion: The distance by VBN and lesion diameter were predictive of the diagnostic rates of primary lung cancer by bronchoscopy in patients with peripheral lung lesions.
Time-Fractional Landau-Khalatnikov Model Applied To Numerical Simulation of Polarization Switching In Ferroelectrics
Authors: Maslovskaya Anna , Moroz Lubov 
Published: January 2022

Abstract
Ferroelectrics are complex structured media that exhibit fractal properties and memory effects in terms of a number of dynamic characteristics. The most relevant applications of ferroelectrics in science and technology are associated with the primary mechanisms of polarization switching and domain structure dynamics induced by external exposure. In this study, we propose a time-fractional modification of the Landau-Ginzburg-Devonshire-Khalatnikov model to describe the dynamics of ferroelectric polarization switching. To solve the time-fractional cubic-quintic partial differential equation numerically, a computational scheme is derived. The technique combines an iterative procedure and an implicit finite-difference scheme based on an approximation of Caputo derivative. A series of computational experiments are presented to visualize polarization switching characteristics on the example of thin films of barium titanate. A variation of the order of fractional derivative as a numerical characteristic of the memory effect allows one to "adjust" suitable regimes of simulated dynamical system. The obtained findings indicate that the generalized time-fractional model can provide better reproduction of experimental data in comparison with the classical analogue.
Evaluating Nanobiomaterial induced DNA strand breaks using the alkaline Comet Assay
Authors: Tutty Melissa Anne , Vella Gabriele , Prina-Mello Adriele , Weimann Martin , Vennemann Antje 
Published: January 2022

Abstract
Due to their unique chemical and physical properties, nanobiomaterials (NBM’s) are extensively studied for applications in medicine and drug delivery. Despite these exciting properties, their small sizes also make them susceptible to toxicity. Whilst nanomaterial immunotoxicity and cytotoxicity are studied in great depth, there is still limited data on their potential genotoxicity, or their potential to cause DNA damage. In the past two years new medical device regulations, which come into place in 2020, have been developed. These require the assessment of long-term NBMs exposure, and also drug delivery systems and nanomedicines, therefore in recent years increased attention is being paid to genotoxicity screening of these materials. In this article, we assess three different particle formulations which different uses, a bio-persistent gold nanoparticle (AuNP), an IR-780 dye loaded liposome which is used in deep tissue imaging, an unloaded PACA polymeric nanoparticle used as a drug delivery system, and two loaded PACA NBMs (CBZ and NR668) for their potential to cause DNA strand breaks using the alkaline Comet Assay and discuss the current state of genotoxicity testing for nanomaterials.
Research of Hyperbaric Oxygen Pretreatment to Improve The Lower Body Negative Pressure Endurance of Pilots with Poor Acceleration Endurance
Authors: Ma Mengyu , Liu Hongjin , Fu Zhaojun , Liu Qiming , Sun Rongli 
Published: January 2022

Abstract
Background: To investigate the effect of hyperbaric oxygen preconditioning (HBOP) on improving the lower body negative pressure endurance of pilots with poor acceleration endurance.Methods：30 pilots with poor acceleration endurance were subjected to multiple HBO（hyperbaric oxygen ,HBO） cabin and 3 times HBO exposure. Oxygen inhalation was performed for 95min, once a day (time was fixed at 14:30-16:15 PM in the HBO cabin), for consecutive 3d. The lower body negative pressure endurance and blood indexes were measured before and after HBOP using the subjects' own before-after control design. Lower body negative pressure endurance adopts stepwise increasing scheme, that is, continuously increasing LBNP load:-20mmHg·1min、-30mmHg·3min、-40mmHg·5min、-50mmHg·7min, etc . Results:The cumulative stress index of subjects before HBOP was (-856.13±273.76min·mmHg), and that after HBOP was (-1237.42±385.50min·mmHg). The cumulative stress index after HBOP increased significantly (t=7.750, P<0.05), namely lower body negative pressure endurance significantly improved. After that, 30 people underwent centrifuge reexamination, and the passing rate of 4.0G10s reached 93.3%.After HBOP, diastolic blood pressure increased (t=-3.252, P<0.05), heart rate slowed down (t=5.493, P<0.05), blood oxygen saturation (SaO2) increased (t=-3.08, P<0.05), but there was no significant difference in systolic blood pressure.Blood lactic acid (BLA) was lower than that before HBOP (t= 2.186, P<0.05); Adrenocorticotropic hormone (ACTH) was not significantly different from that before HBOP. Conclusion:HBOP can improve the lower body negative pressure endurance and centrifuge acceleration endurance of pilots with poor acceleration endurance, and its mechanism may be related to the improvement of autonomic nerve function and the enhancement of human energy metabolism.
Visualizing Omicron: COVID-19 Deaths vs. Cases Over Time
Authors: Arnaout Ramy , Arnaout Rima 
Published: January 2022

Abstract
For most of the COVID-19 pandemic, the daily focus has been on the number of cases, and secondarily, deaths. The most recent wave is caused by the omicron variant, first identified at the end of 2021 and the dominant variant through the first part of 2022. South Africa, one of the first countries to experience and report data regarding omicron (variant 21.K), reported far fewer deaths, even as the number of reported cases rapidly eclipsed previous peaks. However, as the omicron wave has progressed, time series show that it has been markedly different from prior waves. To more readily visualize the dynamics of cases and deaths, it is natural to plot deaths per million against cases per million. Unlike the time-series plots of cases or deaths that have become daily features of pandemic updates during the pandemic, which have time as the x-axis, in a plot of deaths vs. cases, time is implicit, and is indicated in relation to the starting point. Here we present and briefly examine such plots from a number of countries and from the world as a whole, illustrating how they summarize features of the pandemic in ways that illustrate how, in most places, the omicron wave is very different from those that came before. Code for generating these plots for any country is provided on an automatically updating GitHub repository (https://github.com/rarnaout/Covidcycles).
Association of Circadian Rhythm With Mild Cognitive Impairment Among Pneumoconiosis Workers in Hong Kong: a Cross-sectional Study
Authors: Huang Bixia , Liao Gengze , LEE Priscilla Ming Yi , Chan Chi Kuen , Tai Lai-bun , Tsang Chun Yuk Jason , Leung Chi Chiu , Tse Lap Ah 
Published: January 2022

Abstract
Background Weakened circadian rhythm was associated with mild cognitive impairment (MCI) in the general population, but it remains unclear among pneumoconiosis patients and this knowledge gap was rectified in this study.Methods This cross-sectional study consisted of 186 male pneumoconiosis patients and 208 age-matched healthy community men who wore a wrist actigraphy for 168 hours and completed a face-to-face questionnaire containing information on sociodemographic, lifestyle behavior, and anthropometric measurements. Circadian activity rhythm was characterized by a cosine model and the parameters included percent rhythm, amplitude, the midline estimating statistic of rhythm (MESOR), and acrophase. Values below the corresponding medians of the circadian rhythm parameters represented weakened circadian rhythm. Cognitive function and MCI or cognitive impairment was assessed by the Cantonese version of Mini-Mental State Examination (CMMSE). Multivariate logistic regression models were used to evaluate the relationships of circadian rhythm with the prevalence of MCI and composite outcome of MCI plus cognitive impairment.Results The mean age of the pneumoconiosis patients was 71.3 ± 7.8 years with the prevalence of MCI and composite outcome of 38.7 % and 50.0 %. Pneumoconiosis patients had worse cognition and dampened circadian rhythm than the community referents. Compared with the community referents or pneumoconiosis patients with robust circadian rhythm, pneumoconiosis patients with weakened circadian rhythm was consistently associated with increased risk of MCI and composite outcome, though the significant association was only for MESOR and the composite outcome with an adjusted OR of 2.01 (95%: 1.03-3.94). A delayed phase of circadian rhythm was insignificantly associated with MCI and composite outcome.Conclusions Weakened circadian activity rhythm among pneumoconiosis workers was positively associated with the prevalence of MCI and composite outcome. Our study suggests that intervention on improving circadian rhythm may mitigate the cognitive deterioration in pneumoconiosis workers.
Diversity and Characterization of Larval Habitats of Anopheles Mosquitoes in Urban Areas in Benin: Influence of Physicochemical and Bacteriological Factors on Larval Density
Authors: Hessou-Djossou Donald , Djègbè Innocent , Ahadji-Dabla Koffi Mensah , Nonfodji Odilon M. , Tchigossou Geneviève , Djouaka Rousseau , Cornelie Sylvie , Djogbenou Luc , Akogbeto Martin , Chandre Fabrice 
Published: January 2022

Abstract
Background: The implementation of antilarval strategies in the fight against malaria vectors requires fundamental knowledge of their oviposition sites. This study aimed to assess the spatial and temporal distribution of Anopheles breeding sites as well as the influence of abiotic and biotic factors on the proliferation of larvae in urban and non-urban environments in Benin.Methods: Larval surveys were carried out during the rainy and dry seasons in urbanized and non-urbanized areas in the cities of Parakou, Natitingou, Bohicon and Cotonou in Benin. The Anopheles larval breeding sites were georeferenced and characterized by their nature, typology, physicochemical (pH, temperature, dissolved oxygen, conductivity, turbidity, salinity) and biological attributes (larval density and coliform density).Results: A total of 198 different types and positive breeding sites for Anopheles larvae were identified. These include 163 (82.3%) breeding sites during the rainy season and 35 (17.7%) during the dry season. Out of these habitats, 61.9% were located in urbanized areas with a predominance of puddles. The Principal Component Analysis (PCA) revealed a high positive correlation of larval density with temperature, dissolved oxygen and salinity in the coastal zone (Cotonou). In addition, the cross-analysis of the microbiological results with the larval density has generally shown a significant negative correlation between the larval productivity of anopheles breeding sites and their faecal coliforms load. Conclusion: This study showed multiple larval habitats of Anopheles in urban environments due to several anthropogenic factors and larval adaptation to unusual ecosystems.
Evaluation of Retinal and Optic Disc Vascular Structures in Patients With Familial Mediterranean Fever (Fmf) via Optic Coherence Tomography Angiography
Authors: Bulut Mehmet , Kutluk Oznur , Gedik Birumut , Ucar Ulku , Bozdogan Yigit Caglar , Sari Muhammet Huseyin , Cay Hasan Fatih 
Published: January 2022

Abstract
IntroductionFamilial Mediterranean Fever (FMF) is an auto-immune disease characterized by fever and serositis. With this study, we aimed at comparing the retinal vascular structures of FMF patients and healthy individuals using optic coherence tomography angiography (OCTA) device.Material and Method69 patients with FMF and 52 healthy controls who were matched by age and sex, didn’t have any comorbidities and were admitted to our clinic for routine ophthalmic examination were enrolled in the study. 69 eyes of 69 patients and 52 eyes of 52 healthy controls that were not disqualified due to the exclusion criteria were included in the study. OCTA images of the patients and controls were analyzed.ResultsThe mean age of 69 FMF patients included in the study was 39,86 ± 13,16. 24 were female and 16 were male. Total vascular density of deep capillary plexus (DCP) and vascular density of DCP parafovea of the FMF patients were found to be statistically significantly lower than those of the controls (p=0,045, p=0,034, respectively), while total retinal parafoveal thickness, total retinal perifoveal thickness, outer retinal parafoveal thickness and outer retinal perifoveal thickness were statistically significantly higher in the patient group compared with the control group (p=0,012, p=0,014, p=0,013, p=0,009, respectively).ConclusionWe think that the lower values of retinal vascular density in FMF patients compared with the control group were due to vascular injury caused by primarily inflammation and amyloidosis. Furthermore, it can also be suggested that inflammation had an impact on higher retinal thicknesses in FMF patients.
Hijacking a neurodevelopmental epigenomic program in metastatic dissemination of medulloblastoma
Authors: Zou Han , Poore Bradley , Brown Emily , Qian Jieqi , Xie Bin , Asimakidou Evridiki , Razskazovskiy Vladislav , Ayrapetian Deanna , Sharma Vaibhav , Xia Shunjin , Liu Fei , Chen Apeng , Guan Yongchang , Li Zhengwei , Wanggou Siyi , Saulnier Olivier , Ly Michelle , Fellows-Mayle Wendy , Xi Guifa , Tomita Tadanori , Resnick Adam , Mack Stephen , Raabe Eric , Eberhart Charles , Sun Dandan , Stronach Beth , Agnihotri Sameer , Kohanbash Gary , Lu Songjian , Herrup Karl , Rich Jeremy , Gittes George K. , Broniscer Alberto , Hu Zhongliang , Li Xuejun , Pollack Ian , Friedlander Robert , Hainer Sarah , Taylor Michael , Hu Baoli 
Published: January 2022

Abstract
How dysregulation of neurodevelopment relates to medulloblastoma (MB), the most common pediatric brain tumor, remains elusive. Here, we uncovered a neurodevelopmental epigenomic program being hijacked to induce MB metastatic dissemination. Unsupervised analyses by integrating publicly available datasets with our newly generated data revealed that SMARCD3/BAF60C regulates DAB1-mediated Reelin signaling in Purkinje cell migration and MB metastasis by orchestrating cis-regulatory elements (CREs) at the DAB1 locus. We further identified that a core set of transcription factors, enhancer of zeste homolog 2 (EZH2) and nuclear factor I X (NFIX), coordinates with the CREs at the SMARCD3 locus to form a chromatin hub for controlling SMARCD3 expression in the developing cerebellum and metastatic MB. Elevated SMARCD3 activates Reelin/DAB1-mediated Src kinase signaling, resulting in MB response to Src inhibition. These data deepen our understanding of how neurodevelopmental programming influences disease progression and provide a potential therapeutic option for MB patients.
Depletable Peroxidase-like Activity of Fe3O4 Nanozymes Accompanied with Phase Transformation Triggered by Separate Migration of Electron and Iron Ion
Authors: Zhang Yu , Dong Haijiao , Du Wei , Dong Jian , Kong Fei , Cheng Wenlong , Ma Ming , Gu Ning 
Published: January 2022

Abstract
As the pioneering Fe3O4 nanozymes, their explicit peroxidase (POD)-like catalytic mechanism remains elusive. Although many studies have proposed the surface Fe2+-induced Fenton-like reactions accounting for their POD-like activity, few focus on the internal atomic changes and their contribution to the catalytic reaction. Here we report that Fe2+ within Fe3O4 transfers electrons to the surface via the Fe2+-O-Fe3+ chain, regenerating the surface Fe2+ and enabling a sustained POD-like catalytic reaction. This process occurs with the outward migration of excess oxidized Fe3+ from the lattice, which is a rate-limiting step. After prolonged catalysis, Fe3O4 nanozymes suffer the phase transformation to γ-Fe2O3 with a depletable POD-like activity. This self-depleting characteristic of nanozymes with internal atoms involved in electrons transfer and ion migration is well-validated on lithium iron phosphate nanoparticles. We reveal a key yet ever ignored issue concerning the necessity of considering both surface and internal atoms when designing, modulating, and applying nanozymes.
Computational Fluid Dynamic simulation of the supersonic CO2 expansion during champagne cork popping
Authors: Benidar Abdessamad , Georges Robert , CORDIER Daniel , Liger-Belair Gérard 
Published: January 2022

Abstract
Behind the iconic “pop!" accompanying the uncorking of a champagne bottle hides a gas flow of surprising complexity. Its modeling is made delicate by its supersonic nature, its interaction with the cork stopper, the eminently unsteady character of the flow escaping from the bottle, and the continuous change of the geometry of the computational flow domain due to the displacement of the cork. Our numerical simulations reveal the formation, evolution and dissipation of shock wave patterns during the first millisecond following cork popping. A first crown-shaped shock wave pattern develops radially, then followed by the formation of a detached shock wave, or bow shock, induced by the presence of the cork in the axial path of the supersonic gas. The numerical simulations correctly reproduce the position of the bow shock previously observed through high-speed imaging.
Flood Vulnerability Mapping of River Ngadda Using Geospatial and Remote Sensing Techniques Maiduguri Metropolis, Borno State.
Authors: Obroh Oveka , Sambo Garba 
Published: January 2022

Abstract
Flood disaster has been threating to animal, plants, land, human and non-human resources over the years. This have increased concerns on the need to investigate various vulnerable areas to flood for decision making. As a mitigative measures to loss of lives, properties, and livelihood which could arise from flood disaster, understanding zones at risk to flooding is vital to support all stages of disaster management including early warning and planning. River Ngadda is a major river within Maiduguri metropolis. Its plains and banks are known to have different activities and resources. When the river overflows its shores, neighboring lands could suffer destruction, there could be loss of lives and properties. This project aims to investigate flood vulnerability along River Ngadda identifying the shores, plains, and banks of the river at risk. The research adopts ground truth information, the use of Aster DEM (30m) and Sentinel 2 (10m) Satellite Imagery dataset through Geospatial and Remote Sensing techniques to assess undulating nature of the area under study, identify areas liable to flood and analyzed the flood plain. Further analysis was carried out to obtain the Land use and Landcover (LULC) within the study area using a supervised classification and areas at various flood risk were also identified. This research results presented the river plains, flood vulnerable impact on LULC, it was found that 13.17 hectares of built-up areas fall within the very high flood risk zone, over sixty six percent (66%) of the study area appears fall within the moderate to very high vulnerable flood risk zone and the production of the flood plains, LULC, risk and hazard map of the study area was presented. This result could aid early warning, planning and decision making for River Ngadda and its environs.