bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification, phylogenetic analysis, and expression profiles of trihelix transcription factor family genes in quinoa (Chenopodium quinoa Willd.) under abiotic stress conditions
Authors: Li Kuiyin , Fan Yue , Zhou Guangyi , Liu Xiaojuan , Chen Songshu , Chang Xiangcai , Wu Wenqiang , Duan Lili , Yao Maoxing , Wang Rui , Wang Zili , Yang Mingfang , Ding Yanqing , Ren Mingjian , Fan Yu , Zhang Liyi 
Published: July 2022

AbstractBackgroundThe trihelix family of transcription factors plays essential roles in the growth, development, and abiotic stress response of plants. Although several studies have been performed on the trihelix gene family in several dicots and monocots, this gene family is yet to be studied inChenopodium quinoa(quinoa).ResultsIn this study, 47C. quinoatrihelix (CqTH) genes were in the quinoa genome. Phylogenetic analysis of the CqTH and trihelix genes fromArabidopsis thalianaandBeta vulgarisrevealed that the genes were clustered into five subfamilies: SIP1, GTγ, GT1, GT2, and SH4. Additionally, synteny analysis revealed that the CqTH genes were located on 17 chromosomes, with the exception of chromosomes 8 and 11, and 23 pairs of segmental duplication genes were detected. Furthermore, expression patterns of 10 CqTH genes in different plant tissues and at different developmental stages under abiotic stress and phytohormone treatment were examined. Among the 10 genes,CqTH02,CqTH25,CqTH18,CqTH19,CqTH25,CqTH31, andCqTH36, were highly expressed in unripe achenes 21 d after flowering and in mature achenes compared with other plant tissues. Notably, the 10 CqTH genes were upregulated in UV-treated leaves, whereasCqTH36was consistently upregulated in the leaves under all abiotic stress conditions.ConclusionsThe findings of this study suggest that gene duplication could be a major driver of trihelix gene evolution in quinoa. These findings could serve as a basis for future studies on the roles of CqTH transcription factors and present potential genetic markers for breeding stress-resistant and high-yielding quinoa varieties.
Evaluating the cardioprotective effect of metformin on myocardial ischemia–reperfusion injury using dynamic 18F-FDG micro-PET/CT imaging
Authors: Su Hang , Lu Diyu , Shen Mingkui , Feng Li , Xu Chuangye 
Published: July 2022

Abstract
Background
The molecular mechanisms of protective effect of metformin (Met) on ischemic myocardium have not been fully understood. This study aims to evaluate the cardioprotective effect of metformin on myocardial ischemia–reperfusion injury (MIRI) in rat models at different time points using dynamic 18F-FDG micro-PET/CT imaging.

Methods
The I/R injury model in SD rats was established by ligation of left anterior descending coronary artery near the pulmonary arch root for 30 min. SD rats (n = 12) were randomly divided into 2 groups: Control group (n = 6) without any intervention and Met group (n = 6) with oral administration of metformin (50 mg/kg) twice a day. Gated 18F-FDG (40Mbq) micro-PET/CT imaging was performed for 10 min at different time points (day 1st, day 7th, day 14th and day 30th after operation). Volumes of interest were drawn to identify different myocardium regions (ischemia center, peri-ischemia area and remote area). Standardized uptake values (SUVs) (SUVmean and SUVmax) were analyzed to evaluate the FDG uptake activity, and then the center/remote ratio was calculated. In addition, the left ventricular (LV) end-diastolic volume (EDV), end-systolic volume (ESV) and LV ejection fraction (LVEF) were obtained. On the 30th day, all rats were scarified and myocardial ischemia was analyzed by HE staining and confirmed by pathology.

Results
In the Control group, the center/remote ratio showed no obvious change trend at each time point after reperfusion, while the LV EDV increased gradually over time, and they were significantly negatively correlated (r = − 0.507, p < 0.05). In the Met group, the center/remote ratio gradually increased with time, there was no significant correlation between center/remote ratio and LV EDV (r = − 0.078, p > 0.05). On the 30th day, the center/remote ratio of the Met group was significantly higher than that of the Control group (0.81 ± 0.06 vs. 0.65 ± 0.09, p < 0.05), while LV EDV in Met group was significantly lower than in Control group (358.21 ± 22.62 vs. 457.53 ± 29.91, p < 0.05). There was no significant difference of LVEF between Met group and Control group at different time points after reperfusion (p < 0.05). HE staining showed that the myocardial infarction and fibrosis in ischemic center area of the Control group was more serious than that of the Met group.

Conclusions
Met could attenuate the severity of MIRI, delay and prevent the progress of LV remodeling. The cardioprotective progress could be dynamically assessed by 18F-FDG micro-PET/CT imaging.
Nonalcoholic steatohepatitis medical patient journey from the perspective of hepatologists, gastroenterologists and patients: a cross-sectional survey
Authors: Rinella Mary , Cryer Donna R. , Articolo Amy , Fisher Travis , Schneider Jennifer , Nadolsky Karl 
Published: July 2022

Abstract
Background
Nonalcoholic steatohepatitis (NASH), the inflammatory subtype of nonalcoholic fatty liver disease, is underdiagnosed and expected to become the leading indication for liver transplant in the United States. We aimed to understand the medical journey of patients with NASH and role of hepatologists/gastroenterologists in diagnosing and treating patients with NASH.

Methods
A United States population-based cross-sectional online survey was completed by 226 healthcare professionals (HCPs) who treat patients with NASH and 152 patients with NASH; this study focuses on the patient and 75 hepatologist/gastroenterologist HCP respondents. Tests of differences (chi square, t-tests) between respondent types were performed using SPSS.

Results
Most patients reported receiving their diagnosis of NASH from a hepatologist (37%) or gastroenterologist (26%). Hepatologists/gastroenterologists were more likely than other HCPs to use FibroScan (transient elastography) to diagnose NASH and were more likely to distinguish between NASH with or without fibrosis. Hepatologists/gastroenterologists (68%) and patients (52%) agree that hepatologists/gastroenterologists are the primary coordinators of NASH care. The majority of hepatologists/gastroenterologists (85%) are aware of American Association for the Study of Liver Diseases (AASLD) clinical practice guidance, and 86% of those aware consider them when diagnosing patients with NASH. Hepatologists/gastroenterologists most frequently recommended exercise (86%), diet (70%), and supplements (58%) for ongoing management of NASH. Pharmaceutical medications for comorbidities were prescribed by a minority of hepatologists/gastroenterologists for their patients with NASH. Hepatologists/gastroenterologists cite difficulty (67%) or unwillingness (64%) to adhere to lifestyle changes as primary reasons patients with NASH discontinue NASH treatment.

Conclusions
Hepatologists/gastroenterologists are considered the coordinators of NASH care. While recognizing that patient adherence to lifestyle changes is the basis for successful treatment, important barriers limit successful implementation.
Targeting the transcriptional activity of STAT3 by a novel fusion protein
Authors: Chen Yanqiong , Zhang Wenting , Bai Xiufeng , Liu Yi 
Published: July 2022

Abstract
Background
The continuous activation of transcription factors drives many diseases, including tumors, autoimmune disease, neurodegenerative disease, and male infertility. Thus, Blocking the transcriptional activity of these proteins may inhibit disease progression. In this study, we developed a new method to specifically inhibit the activity of the transcription factor STAT3.

Methods
Fusing the transcriptional inhibitory domain KRAB with STAT3 successfully blocked the transcription activity of STAT3 in cancer cells without affecting its function in the mitochondria and lysosomes.

Results
the expression of KRAB-STAT3 fusion protein inhibited the growth of tumor cells.

Conclusions
The KRAB-STAT3 fusion protein provides a novel approach for drug development for the treatment of cancer or autoimmune diseases.
Obstetric fistula repair failure and its associated factors among women underwent repair in Yirgalem Hamlin fistula center, Sidama Regional State, Southern Ethiopia, 2021: a retrospective cross sectional study
Authors: Tadesse Shimelis , Ejigu Neway , Edosa Dejene , Ashegu Tebeje , Dulla Dubale 
Published: July 2022

Abstract
Background
Obstetric fistula repair failure is a combination of unsuccessful fistula closure and/or incontinence following a successful closure. It causes a burden on both the patients and the fistula centers. The aim of this study was to assess the magnitude and associated factors of obstetric fistula repair failure among women who underwent fistula repair at Yirgalem Hamlin fistula center in Southern Ethiopia.

Methods
A facility-based retrospective cross-sectional study was conducted among women who underwent fistula repair at Yirgalem Hamlin fistula center, Southern Ethiopia, during the period from January 2016 to December 2020. All 562 women who underwent fistula repair in the last 5 years were included in the study. The data were collected using a pre-tested checklist from September 22 to October 22, 2021. The data were then imported into EPI info-data version 3.1, exported to SPSS version 25, and analyzed. Descriptive and logistic regression analyses were performed, and the significant statistical test was assessed at a 95% confidence interval. Variables with a p value of < 0.05 in multivariable logistic regression were regarded to have a statistically significant relationship.

Results
The magnitude of obstetric fistula repair failure in this study was 28.8%. Obstetric fistula repair failure was found to be associated with labor duration > 48 h (AOR = 2.037; 95% CI 1.268, 3.272), Goh Type 4 fistulas (AOR = 3.939; 95% CI 1.623, 9.560), fistula size > 3 cm (AOR = 6.627; 95% CI 3.802, 11.554), completely destructed urethra (AOR = 3.192; 95% CI 1.234, 8.256), and bladder catheterization > 14 days (AOR = 2.944; 95% CI 1.380, 6.281).

Conclusions
The magnitude of obstetric fistula repair failure was significantly higher than the World Health Organization standard. Obstetric fistula repair failure had a positive association with a longer duration of labor, Goh Type 4 fistulas, large fistula size, total urethral injury, and a longer period of bladder catheterization. Therefore, the concerned bodies need to implement interventions on factors affecting obstetric fistula repair failure to reduce or prevent the failure of obstetric fistula repair.
Selective sphingosine-1-phosphate receptor 1 modulator attenuates blood–brain barrier disruption following traumatic brain injury by inhibiting vesicular transcytosis
Authors: Zhang Yuan , Wang Lin , Pan Qiuling , Yang Xiaomin , Cao Yunchuan , Yan Jin , Wang Yingwen , Tao Yihao , Fan Runjin , Sun Xiaochuan , Li Lin 
Published: July 2022

Abstract
Background
Traumatic brain injury (TBI) provokes secondary pathological damage, such as damage to the blood–brain barrier (BBB), ischaemia and inflammation. Major facilitator superfamily domain-containing 2a (Mfsd2a) has been demonstrated to be critical in limiting the increase in BBB vesicle transcytosis following brain injury. Recent studies suggest that a novel and selective modulator of the sphingosine-1-phosphate receptor 1 (S1P1), CYM-5442, maintains the integrity of the BBB by restricting vesicle transcytosis during acute ischaemic stroke. In the current study, we investigated whether CYM-5442, evaluated in a short-term study, could protect the brains of mice with acute-stage TBI by reversing the increase in vesicle transport due to reduced Mfsd2a expression after TBI.

Methods
We used the well-characterized model of TBI caused by controlled cortical impact. CYM-5442 (0.3, 1, 3 mg/kg) was intraperitoneally injected 30 min after surgery for 7 consecutive days. To investigate the effect of CYM-5442 on vesicle transcytosis, we downregulated and upregulated Mfsd2a expression using a specific AAV prior to evaluation of the TBI model. MRI scanning, cerebral blood flow, circulating blood counts, ELISA, TEM, WB, and immunostaining evaluations were performed after brain injury.

Results
CYM-5442 significantly attenuated neurological deficits and reduced brain oedema in TBI mice. CYM-5442 transiently suppressed lymphocyte trafficking but did not induce persistent lymphocytopenia. After TBI, the levels of Mfsd2a were decreased significantly, while the levels of CAV-1 and albumin were increased. In addition, Mfsd2a deficiency caused inadequate sphingosine-1-phosphate (S1P) transport in the brain parenchyma, and the regulation of BBB permeability by Mfsd2a after TBI was shown to be related to changes in vesicle transcytosis. Downregulation of Mfsd2a in mice markedly increased the BBB permeability, neurological deficit scores, and brain water contents after TBI. Intervention with CYM-5442 after TBI protected the BBB by significantly reducing the vesicle transcytosis of cerebrovascular endothelial cells.

Conclusion
In addition to transiently suppressing lymphocytes, CYM-5442 alleviated the neurological deficits, cerebral edema and protective BBB permeability in TBI mice by reducing the vesicle transcytosis of cerebrovascular endothelial cells.
Solving an integral equation via $\mathscr{C}^{\star}$-algebra-valued partial b-metrics
Authors: Maheswari J. Uma , Anbarasan A. , Gunaseelan M. , Parvaneh V. , Bonab S. Hadi 
Published: July 2022

AbstractIn this paper, we prove some common coupled fixed-point theorems on complete $\mathscr{C}^{\star}$

C
⋆

-algebra-valued partial b-metric spaces. Some of the well-known facts in the literature are generalized and expanded by the results shown. An example to illustrate our findings is presented. We also explore some of the applications of our key results.
Biomechanical analysis of effective mandibular en-masse retraction using Class II elastics with a clear aligner: a finite element study
Authors: Wang Qiuyu , Dai Danni , Wang Jianrong , Chen Yanfang , Zhang Chao 
Published: July 2022

Abstract
Background
This study aimed to evaluate the displacement and stress distribution of mandibular dentition by various positions of the Class II elastics during en-masse retraction in clear aligner therapy.

Methods
Models including a mandibular dentition (without first premolars), periodontal ligament (PDL), mandible, as well as attachments, aligners and buttons were constructed and imported into Ansys Workbench 2019 (ANSYS, USA) to generate the three-dimensional (3D) finite element model. Six combinations were created: (1) aligner alone (control), (2)-(5) Class II elastics with buttons placed on the mesiobuccal (MB), distobuccal (DB), mesiolingual (ML) and distolingual (DL) surface of the mandibular first molar, and (6) Class II elastics with a button on the aligner corresponding to the mesiobuccal surface of the mandibular first molar (AMB). The elastic force was set to 2 N for simulations.

Results
The central incisors appeared lingual tipping in the six models. The lingual crown movement of the central incisors was 0.039 mm, 0.034 mm, 0.034 mm, 0.042 mm, 0.041 mm, and 0.034 mm for control model, MB model, DB model, ML model, DL model, and AMB model, respectively. The first molars showed mesial tipping in the six models. The mesial movement of the mesiobuccal cusps of the first molars was 0.045 mm, 0.060 mm, 0.063 mm, 0.048 mm, 0.051 mm, and 0.055 mm for control model, MB model, DB model, ML model, DL model, and AMB model, respectively.

Conclusions
Class II elastics reduced lingual tipping of anterior teeth but aggravated mesial tipping of posterior teeth. Mesiolingual elastics developed minimum mesial tipping of the posterior teeth. When Class II elastics are required, attaching elastics on the mesiolingual surface of the mandibular first molar is recommended to prevent mandibular anchorage loss.
A single centre case series of microvascular decompression surgery for classical trigeminal neuralgia: functional outcomes and report of a unique conflict
Authors: Abougamil Ahmed B. , Rayan Tarek , Khedr Wael 
Published: July 2022

Abstract
Background
Microvascular decompression surgery for trigeminal neuralgia is now considered the benchmark surgical procedure for intractable trigeminal neuralgia of any variety. The objective of this study is to share our experience in managing classical trigeminal neuralgia with microvascular decompression surgery looking into both immediate and late functional outcomes at 3 and 6 months post op. We also report a unique case of trigeminal neuralgia due to a tributary of the petrosal vein passing through the substance of the trigeminal nerve itself at its root entry zone.

Results
This study included 20 patients; it showed that microvascular decompression surgery of the trigeminal nerve is an effective method for treatment of classic trigeminal neuralgia (p value < 0.001). The trigeminal root was compressed by the superior cerebellar artery in 75% of patients. A vein contributed to the compression in 45% of patients and was the only compressing vessel in 15%.

Conclusion
Microvascular decompression surgery is the treatment of choice in patients with medically refractory trigeminal neuralgia, unless their general condition prohibits it. It’s also worth noting that the normal anatomy of the veins in this region is quite variable and the venous structures causing a neurovascular contact are more or less inconsistent. To the best of our knowledge and despite the wide range and progress in microvascular decompression surgery for trigeminal neuralgia, this is the first reported case in the literature for a neurovascular conflict caused by a vein passing through the trigeminal nerve itself.
Assessing post-cochlear implantation anxiety and correlating it to socio-demographic and clinical characteristics
Authors: Ghaly A. , Nada E. , Rabie S. , Ragaey Y. , Elkhatib H. 
Published: July 2022

Abstract
Background
There is a greater incidence of mental health problems among children with hearing impairment compared to controls. A high proportion of children with hearing impairment experience delays in understanding, recognizing, and using emotional expression. There are additional difficulties in post-cochlear transplant patients that may increase the risk of developing psychopathology, such as anxiety disorder.

Results
Forty mothers of children who underwent cochlear implantation surgery responded to the Spence Preschool Anxiety Scale. Socio-demographic, medical, and perioperative data of children were obtained from medical records. On the Spence Preschool Anxiety Scale, 21 out of 40 children (52.25%) have a positive total score. On obsessive–compulsive disorder, physical injury fears, generalized anxiety, separation anxiety, and social anxiety subscales, the numbers of children who scored positive are 23 (57.5%), 18 (45%), 18 (45%), 10 (25%), and 6 (15%), respectively.

Conclusion
Anxiety disorders are common in post-cochlear transplant children.
Effect of Levothyroxine Supplementation on the Cardiac Morphology and Function in Patients With Subclinical Hypothyroidism: A Systematic Review and Meta-analysis
Authors: Wang Xichang , Wang Haoyu , Li Qiuxian , Wang Ping , Xing Yumin , Zhang Fan , Li Jiashu , Shan Zhongyan 
Published: July 2022

Abstract

Context
The impact of abnormal thyroid hormone levels on the cardiovascular system has been explored for decades. Recent emerging evidence suggests that subclinical thyroid dysfunction, especially subclinical hypothyroidism (SCH), significantly affects cardiac indices.

Objective
We aimed to determine whether levothyroxine (LT4), commonly used to treat hypothyroidism, affects cardiovascular indices in SCH patients.

Methods
This is a systematic review and meta-analysis. We searched online databases for studies analyzing cardiac morphology and functional changes in SCH patients before and after LT4 supplementation. A total of 294 SCH patients participated and finished the follow-up. The standard mean difference and 95% CI were calculated in fixed or random-effects models. The clinical outcomes analyzed in this study included 18 indicators, mainly covering cardiac morphology, myocardial performance (including various indicators of systolic and diastolic function), mitral wave flow, and systemic vascular resistance.

Results
A total of 11 studies met our search criteria. All studies explicitly mentioned that serum thyrotropin levels decreased to normal at follow-up. Our results suggest that the cardiac output (CO), left ventricular ejection fraction (LVEF), and the ratio of peak E velocity/peak A velocity were all significantly increased after LT4 supplementation compared with the baseline level. However, we found no clear evidence of significant morphological changes in the heart.

Conclusion
Judging from the obvious changes in the CO, LVEF, and E/A ratio, LT4 supplementation can effectively improve the cardiac systolic and diastolic dysfunction prevalent in SCH patients. This study provides evidence of the recommendation for LT4 supplementation in adult SCH patients.
Awareness of Polish society about complications and contraindications in teeth whitening
Authors: Kula Łucja , Jaślar Tomasz , Szmidla Zuzanna , Mocny-Pachonska Katarzyna 
Published: July 2022

Introduction and aim: Teeth whitening, which has a positive effect on the quality of life and the human psyche but is associated with side effects and contraindications, is becoming increasingly popular and widespread. The aim of the study was to collect and analyse data concerning the awareness of the Polish population of complications and contraindications to tooth whitening.
Material and methods: A survey was conducted using a Google document among teeth whitening and non-whitening individuals. Link to the survey posted on various social groups.
Results: People who whiten their teeth in a dental office are the group that showed the highest awareness about the side effects and contraindications of teeth whitening. People who do not whiten their teeth are more aware than people who whiten outside the dental surgery. The popularised slogans such as the possibility of tooth hypersensitivity, weakening of the enamel or the necessity to follow a "white diet" are known to each study group. The issues connected with the dentist's office were the least popular - persons whitening their teeth outside the dentist's office are not aware of the necessity to professionally clean their teeth before this procedure. None of the study groups have knowledge about the temporomandibular joint problem as a contraindication to teeth whitening.
Conclusions: The Polish population shows an average knowledge of teeth whitening. The basic contraindications and complications are known to a broader group of respondents, but there are aspects of whitening about which the knowledge of respondents is low and requires popularisation. In addition, the survey indicates that more research is needed into the relationship between the temporomandibular joint and tooth whitening.