bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the prognostic impact of SP263-evaluated PD-L1 expression in patients with non-small cell lung cancer (NSCLC) stage III treated with radio-chemotherapy
Authors: Wagner Jan , Roeper Julia , Heukamp Lukas , Falk Markus , Willborn Kay , Griesinger Frank 
Published: August 2022

Abstract
Background
The PACIFIC study showed that patients with NSCLC after radio-chemotherapy derived a benefit in PFS and OS when treated with durvalumab, a PD-L1-inhibitor, vs placebo. In a post-hoc analysis this effect was limited to patients with a PD-L1 expression of > 1%, while patients < 1% did not benefit from durvalumab, partly because the outcome in the observational control arm was surprisingly favorable. Thus, it could be speculated that lack of PD-L1 expression confers a favorable outcome in stage III NSCLC.
Intention
We retrospectively analyzed PFS and OS in a group of 99 patients with stage III NSCLC homogeneously treated and not progressing after radio-chemotherapy. In all patients, PD-L1 expression was evaluated and outcome in the groups of PD-L1 < 1% and PD-L1 ≥ 1% was compared.
Methods
Clinical data, PD-L1 expression, predictive blood-markers and outcome were retrospectively captured from primary cases of the certified lung cancer center Oldenburg. Statistical analyses using log-rank test were performed.
Results
The median OS of the patients with an expression of PD-L1 < 1% was 20 months (CI 10.5–29.5) and with an expression ≥ 1% 28 months (CI 16.5–39.2), (p = 0.734). The median PFS of the patients with an expression of PD-L1 < 1% was 9 months (CI 6.3–11.6) and with an expression ≥ 1% 12 months (CI 9.8–14.2), not statistically significant (p = 0.112). The blood parameters had no significant impact on the OS.
Conclusion
In this retrospective cohort of stage III patients, the assumption that lack of PD-L1 expression represents a favorable prognostic factor after radio-chemotherapy vs. PD-L1 expression > 1% was not confirmed.
Genetic Disorders and Pregnancy Outcomes of non-immune Hydrops Fetalis in a Tertiary Referral Center
Authors: Guo Danhua , He Shuqiong , Lin Na , Dai Yifang , Li Ying , Xu Liangpu , Wu Xiaoqing 
Published: August 2022

Abstract
Background: Non-immune hydrops fetalis (NIHF) is a nonspecific symptom with a wide variety of disorders, and the prognosis depends on the underlying etiology. The aim of the study was to investigate the incidence of chromosomal abnormalities and α0/α0 thalassemia in non-immune hydrops fetalis NIHF pregnancies in South China.Methods: We retrospectively reviewed NIHF pregnancies referred to the Fujian Provincial Maternity and Children's Hospital during 2014 and 2018. Pregnancies with maternal alloimmunization were excluded. Thalassemia genotyping and routine karyotyping were performed in all enrolled 129 cases, and chromosomal microarray analysis was performed in 35 cases with normal karyotype. Results: α0/α0 thalassemia was detected in 34.9% (45/129) of the cohort, while chromosomal abnormalities were detected in 29.5% (38/129), including 37 cases of aneuploidy and a single case of imbalanced structural abnormality. In 35 cases with normal karyotype, chromosomal microarray analysis (CMA) revealed no additional pathogenic variations. The rates of chromosomal abnormalities subsided with the onset of trimester, with 65%, 30.1%, and 8.3% in the first, second, and third trimester, respectively (p＜0.05 ). Among 46 (35.6%) cases of unknown etiology, 23 cases accompanied with ultrasonic abnormalities resulted in poor outcome, while 7 cases presented only with multiple ascites that resolved or remitted prior to birth, normal development was observed during 3-4 years follow-up.Conclusion: α0/α0 thalassemia and chromosomal abnormalities are the most common genetic etiologies of NIHF in South China. Generalized skin edema and accompanying ultrasonic abnormalities are predictive of adverse outcomes, and intensive monitoring should be provided to NIHF for better pregnancy management.
Transcriptome analysis of critical genes related to flowering in Mikania micrantha at different altitudes provides insights for a potential control
Authors: Liang Chen , Liu Ling , Zhang Zhixiao , Ze Sangzi , Pei Ling , Feng Lichen , Ji Me , Yang Bin , Zhao Ning 
Published: August 2022

Abstract
Background: Mikania micrantha is a vine with strong invasion ability, and its strong sexual reproduction ability is not only the main factor of harm, but also a serious obstacle to control. Therefore, inhibiting the flowering and seed production of M. micrantha is an effective strategy to prevent M. micrantha from continuing to invade.Result: The flowering number of M. micrantha is different at different altitudes. A total of 67.01 Gb clean data were obtained from nine cDNA libraries, and more than 83.47% of the clean reads were mapped to the reference genome. In total, 5878 and 7686 significantly differentially expressed genes (DEGs) were found in E2-vs-E9 and E13-vs-E9, respectively. Based on the background annotation and gene expression, some candidate genes related to flowering pathway were initially screened, their expression levels in the three different altitudes in flower bud differentiation showed the same trend, that is, at an altitude of 1300 meters, the flower integration gene and flower meristem gene were down-regulated (such as SOC1 and AP1), and the flowering inhibition gene was up-regulated (such as FRI and SVP). Additional, the results show that there were many DEGs involved in hormone signal transduction pathway in the flower bud differentiation of M. micrantha at different altitudes.Conclusions: Our results provide abundant sequence resources for clarifying the underlying mechanisms of the flower bud differentiation and mining the key factors to inhibit the flowering and seed production of M. micrantha, so as to provide technical support for the discovery of an efficient control method.
The usefulness of the CASPRI and the GO-FAR scores for predicting the neurological prognoses of patients who recovered spontaneous circulation after in-hospital cardiac arrest.
Authors: Jung Jonghee , Lee Min Jee , Ryu Ji Ho , Shon Seungwoo , Min Munki , Hyun Tae Gyu , Chun Mose , Lee Daesup 
Published: August 2022

Abstract
Background The Cardiac Arrest Survival Post-Resuscitation In-hospital (CASPRI) and Good Outcome Following Attempted Resuscitation (GO-FAR) scores are used for predicting the outcomes of cardiac arrest. The CASPRI score was developed for the prediction of neurological outcomes after in-hospital cardiac arrest (IHCA), whereas the GO-FAR score is used to predict survival after cardiac arrest. The aim of this study was to confirm the usefulness of the CASPRI and GOFAR scores for predicting the neurological prognoses in-hospital cardiac arrest (IHCA) patients immediately after the return of spontaneous circulation (ROSC). Methods This was a retrospective analysis of patient data from a tertiary general hospital. A total of 488 adult patients who had IHCA and achieved sustained ROSC from September 2016 to August 2021 were analyzed to compare effectiveness of the CASPRI and GO-FAR scores related to neurologic prognoses. The primary outcome was Cerebral Performance Category (CPC) score at discharge. Results Of the 488 included patients, 85 (20.8%) were discharged with good prognoses (CPC score 1 or 2). The area under the receiver operating characteristic curve of CASPRI score for the prediction of a good neurological outcome was 0.748 (OR, 0.86; 95% CI, 0.82-0.90), whereas that of GO-FAR score was 0.668 (OR, 0.95; 95% CI, 0.93-0.97). Conclusion The CASPRI and GO-FAR scores include factors related to a patient's clinical condition and situations when CPR occurred. These scoring systems can be used for timely and satisfactory prediction of the neurological prognoses of IHCA patients after ROSC.
The effect of mindfulness-based stress reduction program on metacognitive awareness, rumination, and self-compassion in menopausal women
Authors: Hassannejad Mona , Yekta Mehrnaz Azad , Sevini Sajad Naderi , Khani Abbas 
Published: August 2022

Abstract
Women spend more than a third of their lives during menopause and estrogen deficiency. In addition, these neuroendocrine changes affect a woman's physical and mental health by reducing her fertility and menstrual function. Therefore, this study aims to investigate the effect of mindfulness-based stress reduction on metacognitive awareness, rumination, and self-compassion in postmenopausal women. This research was applied in terms of purpose and semi-experimental in terms of implementation method. The statistical population of this study included all postmenopausal women referred to the Valiasr Hospital Health Center in Iran in the fall of 2021; 70 women were selected by convenience sampling and randomly assigned to experimental (35) and also control (35) groups to investigation. According to the findings of paper, data analysis results using multivariate analysis of covariance (MANOVA) showed that this research effectively helps to increase metacognitive awareness and also increase self-compassion, and also it can decrease rumination in postmenopausal women.
Pre-treatment metastatic growth rate predicts clinical outcome of metastatic renal cell carcinoma treated with nivolumab
Authors: Matsumura Soichi , Kato Taigo , Kujime Yuma , Kitakaze Hiroaki , Nakano Kosuke , Hongo Sachiko , Yoshioka Iwao , Okumi Masayoshi , Nonomura Norio , Takada Shingo 
Published: August 2022

Abstract
Background: Immune checkpoint inhibitors (ICIs) have been approved for the treatment of metastatic renal cell carcinoma (mRCC). However, the response rate is still limited, and it is urgent to pursue novel and concise markers of responses to ICIs that allow the determination of clinical benefits. It was reported that the metastatic growth rate (MGR) is an independent predictor of response for anticancer therapy in some types of cancer. Methods: We investigated pre-treatment MGR before starting nivolumab for mRCC patients between September 2016 to October 2019. We examined clinicopathological factors including MGR and analyzed the correlation between pre-treatment MGR and clinical efficacy of nivolumab.Results: Of all patients, the median age was 63 years (range, 42 - 81), and the median observation period was 13.6 months (range, 1.7 - 40.3). Twenty-three patients and sixteen patients were classified as the low and the high MGR group, respectively, with the cutoff value of 2.2 mm/month. Progression-free survival (PFS) and overall survival (OS) were significantly better in patients in the low MGR group (p = 0.005 and p = 0.01). Importantly, in multivariate analysis, only the high MGR was significantly associated with a decreased of PFS (Hazard ratio (HR): 2.69, p = 0.03) and OS (HR: 5.27, p = 0.02).Conclusions: Pre-treatment MGR may serve as the simple surrogate marker using the imaging tests and predict clinical responses to anti-PD-1 monotherapy.
Morphological characterization, propagation strategy and identification of Boucerosia frerei accessions for their hanging basket exploitation- an attractive succulent with star-shaped flowers from the Northern Western Ghats of India
Authors: Bodhe M M , Patil A S , Bhosale V D , Dhurgude P N , Shelar P S , Ghanawat N A , Chavan Jaykumar J. 
Published: August 2022

Abstract
Boucerosia frerei (G. D. Rowley) Mieve & Liede (formerly Frerea indica; family-Apocynaceae) is one of the little known, endemic and critically endangered succulent herbs with pretty flowers distributed sparsely in the Northern Western Ghats of India. Naturally, the plant shows phenotypic variation in numerous qualitative and quantitative characters especially stem, leaves and flowers. Consequently, this is challenging and stimulating taxa that merit considerable further attention of taxonomists, horticulturalist, gardeners, plant enthusiast etc. The present investigation describes the first report of collection, phenotypic characterization, commercially practicable propagation strategy development and identification of the accession for their usage in hanging baskets for B. frerei. The results highlighted some of the important and interesting features of this plant. A total of 32 morphological traits (9 qualitative and 23 quantitative) were noted for five accessions collected from Kate’s Point, Mahabaleshwar (KP), Meruling, Satara (MS), Sajjangad, Satara (SS), Purandar, Pune (PP) and YCIS, Satara (YCIS) and evaluated using ANOVA, PCA and cluster analysis. Ten morphological traits viz. plant height, stem color, number of nodes, number of roots, number of leaves, number of flowers, size, shape, architecture and seed weight contributed to the variation between the accessions. For succulents to be used for hanging baskets, plant height, number of leaves and floral and floral attributes (number, color, size, shape and architecture) are among the most important selection traits. Morphological characterization identified the Meruling, Satara (MS) as the potential accession for hanging baskets. A combination of soil, coconut coir and vermiculite (3:1:1) escalated the phenotypic traits in the aerial parts in hanging basket cultivation. Among the propagation techniques employed, vegetative propagation served superior over tissue-culture technique for large-scale propagation for its conservation and availing the stock material to floriculture industry.
Genome-Wide Identification and Characterization of lncRNAs in sunflower endosperm
Authors: Yu Shuai , Zhang Zhichao , Li Jing , Zhu Yanbin , Yin Yanzhe , Zhang Xiaoyu , Dai Yuxin , Zhang Ao , Li Cong , Zhu Yanshu , Fan Jinjuan , Ruan Yanye , Dong Xiaomei 
Published: August 2022

Abstract
Background: Long non-coding RNAs (lncRNAs), as important regulators, play important roles in plant growth and development. The expression and epigenetic regulation of lncRNAs remain uncharacterized generally in plant seeds, especially in the transient endosperm of the dicotyledons. Results: In this study, we identified 11,840 candidate lncRNAs in 12 day-after-pollination sunflower endosperm by analyzing RNA-seq data. These lncRNAs were evenly distributed in all chromosomes and had specific features that were distinct from mRNAs including tissue-specificity expression, shorter and fewer exons. By GO analysis of protein coding genes showing strong correlation with the lncRNAs, we revealed that these lncRNAs potential function in many biological processes of seed development. Additionally, genome-wide DNA methylation analyses revealed that the level of DNA methylation at the transcription start sites was negatively correlated with gene expression levels in lncRNAs. Finally, 36 imprinted lncRNAs were identified including 32 maternally expressed lncRNAs and four paternally expressed lncRNAs. In CG and CHG context, DNA methylation levels of imprinted lncRNAs in the upstream and gene body regions were slightly lower in the endosperm than that in embryo tissues, which indicated that the maternal demethylation potentially induce the paternally bias expression of imprinted lncRNAs in sunflower endosperm. Conclusion: Our findings not only identified and characterized lncRNAs on a genome-wide scale in the development of sunflower endosperm, but also provide novel insights into the parental effects and epigenetic regulation of lncRNAs in dicotyledonous seeds.
Study on Kinematic Structure Performance and Machining Characteristics of Machine Tools
Authors: Chan Tzu-Chi , Chang Chia-Chuan , Lin Han-Huei , Ullah Aman 
Published: August 2022

Abstract
The rigidity and natural frequency of machine tools considerably influence cutting and generate great forces when in contact with the workpiece. The poor static rigidity of these machines can cause deformations and destroy the workpiece. If the natural frequency of the machines is low or close to the commonly used cutting frequency, they vibrate considerably, resulting in poor workpiece surfaces and thus shortening the lifespan of the tool. In this study, the finite element method was mainly used to analyze the structure, static force, modal, frequency spectrum, and transient state of machine tools. The results of the state analysis were verified and compared to the experimental results. The analysis model and conditions were modified to ensure that the analysis results were consistent with the experimental results. Multi-body dynamics analyses were conducted by examining the force of each component and casting of the machine tools and the load of the motor during the cutting stroke. Moreover, an external force was applied to simulate the load condition of the motor when the machine tool is cutting to confirm the feed. In this study, we used topology optimization for effective structural optimization designs. The optimal conditions for topology optimization included lightweight structures, which resulted in reduced structural deformation and increased natural frequency.
Identification of A Six Immune-Related Genes Prognostic Signature and Exploration of Individual Therapies in Young Women with Cervical Cancer
Authors: Bao Huiqiong , Wang Mingbing , Mei Ping 
Published: August 2022

Abstract
Cervical cancer (CC) is a great public health problem worldwide, half of CC patients were diagnosed younger than 50 years. However, there is a lack of special research to predict the prognosis of young CC patients and explore individualized treatment. Hence, it’s imperative to identify prognostic biomarkers and explore novel therapeutic strategies for this population. In view of the importance of tumor immunity, here, we screened differentially expressed immune-related genes (DEIRGs) of young CC patients. Functional analyses (GO and KEGG pathway analysis) were processed. Based on a 6-DEIRGs (namely ANGPTL5, CCL18, LCN6, OLR1, PTH2R and UMODL1) signature, we characterized two immune-related groups of young CC patients with distinct prognosis and immune infiltration features. This prognostic model showed excellent performance in internal and external validation. In terms of potential mechanisms and therapeutic strategies, we evaluated the differences in the gene set enrichment analysis (GSEA) and tumor-infiltrating lymphocytes. The differential immune landscape between the two groups was uncovered. Subsequently, the efficacy of immune checkpoint inhibitors (ICI) and target therapy in the two groups were predicted. Intriguingly, we found that significantly higher expression of pivotal immune checkpoint molecules including costimulatory molecules in low-risk group of young CC patients, suggesting the complexity of tumor immune microenvironment in young patients with CC. In conclusion, we constructed a valuable and feasible signature to predict the prognosis of young CC patients, and risk score is also a useful indicator of individualized treatment for this population.
Novel reconstruction of low-dose DCP-CT images using a regularized least-squares method based on voxel-level TAC correction (RLS-VC)
Authors: Chen Zixiang , Huang Ying , Zhang Na , Zhang Qiyang , Liang Kaizhi , Quan Guotao , Li Xiang , Huang Zhenxing , Liang Dong , Hu Zhanli 
Published: August 2022

Abstract
Dynamic cerebral perfusion computed tomography (DCP-CT) is an advanced imaging technique that helps in the clinical diagnosis of cerebrovascular diseases (CVDs). However, radiation dose deposition during repeated CT scans seriously limits its clinical application. In this study, we propose a regularized least-squares method with high interpretability based on voxel-level time-attenuation curve (TAC) correction (RLS-VC) for DCP-CT image reconstruction with a dual low-dose imaging protocol that involves both sparse sampling and low-mAs X-ray emission. The theory of third-order Hermite interpolation (THI) is applied to voxel-level TAC correction during dynamic image reconstruction. The peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) are used to quantitatively evaluate the proposed method in terms of imaging accuracy and noise reduction, while hemodynamic maps, including cerebral blood flow (CBF) and cerebral blood volume (CBV), are calculated to validate its ability to restore hemodynamic parameters. It is proven that the proposed RLS-VC method for low-dose DCP-CT imaging has better performance than several state-of-the-art dynamic CT imaging methods, including PICCS, ndiNLM and PIDT, as well as the commercial FBP method. It can be expected that the RLS-VC method can play an important role in the promotion of the clinical application of DCP-CT for the diagnosis of CVD.
Participatory Evaluation and Demonstration of Onion Response to Deficit Irrigation at Gibe Woreda, Ethiopia
Authors: Kifle Tamirneh , Mengist Demeke , Gebre Temesgen 
Published: August 2022

Abstract
Water scarcity has become a challenge for agricultural production in the arid area of Ethiopia, particularly in areas characterized by high evaporative demand and low and irregular rainfall. One of the options that can help to save irrigation water under these conditions is the adoption of deficit irrigation by farmers. This study aims participatory evaluation of the deficit irrigation level on onion yield and water use efficiency. To achieve this objective, the experimental was arranged in a randomized complete design with three levels of irrigation treatments and five replications. The treatment was three deficit irrigation levels (70%, 80%, and 100% ETc). The results showed that, deficit irrigation level have a significant difference in plant height, bulb weight, bulb diameter and total yield of onion and water use efficiency. Onion yield has significant difference between 100%ETc and 70%ETc, but there is no significant difference between 100%ETc and 85%ETc. The highest water use efficiency was obtained under70%ETc with significant yield reduction. Therefore, in an area where a sufficient amount of water is available full irrigation is recommended to obtain maximum yield, but in water-scarce areas applying 85%ETc is recommended with non-significant yield reduction.
In-Silico Identification and Characterization of Universal Stress Protein (USP) Gene Family in Triticum aestivum
Authors: Imran Hajira , Aslam Roohi , Gul Alvina , Paracha Rehan Zafar , Munir Faiza , Amir Rabia , Bhatti Muhammad Faraz 
Published: August 2022

Abstract
Climate has changed drastically over the last decade. It is crucial to understand the needs of the plants and their adaptive mechanism that help them survive during adverse environmental conditions. Abiotic stressors mainly salt concentration, osmotic stress, heat stress, drought, flooding, etc. affect plants significantly. In this research work, we identified and characterized wheat's Universal Stress Protein (USP) gene family. In-silico approaches such as identification, gene ontologies, chromosomal mapping, circos, and synteny analysis were used to analyze the reported sequences. The study revealed that the domain architecture plays the most significant role in this family's multi-functional features, which is present in all plants. Moreover, the syntenic relationship revealed the conservancy among the monocot genomes. The role of USP in host cells was explored through studies/tools such as subcellular localization and gene ontologies The presence of several regulatory elements also gave insight into stress-specific modulation and regulation. Furthermore, protein modeling of the TaUSP genes revealed the presence of binding pockets with functionally important amino acids This work led us to report a total of 107 protein sequences on the ABD genome grouped into 34 TaUSP genes. Further instigations such as expression profiling might help verify these genes' stress-specific transcriptional modulation. Hence, this work would be quite useful in developing economically stress-resilient varieties.