bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interferon regulatory factor 1 (IRF-1) promotes intestinal group 3 innate lymphoid responses during Citrobacter rodentium infection
Authors: Schmalzl Angelika , Leupold Tamara , Kreiss Lucas , Waldner Maximilian , Schürmann Sebastian , Neurath Markus F. , Becker Christoph , Wirtz Stefan 
Published: September 2022

AbstractGroup 3 innate lymphoid cells (ILC3s) are crucial mediators of immunity and epithelial barrier function during immune responses against extracellular bacteria. Here, we identify Interferon regulatory factor 1 (IRF-1), a transcription factor previously associated with type 1 immunity, as an essential regulator of intestinal ILC3 accumulation and effector cytokine production. We demonstrate that IRF-1 is upregulated in the context of infection with the enteropathogen Citrobacter rodentium and that its presence is central for anatomical containment and prevention of pathogen dissemination. We furthermore show that IRF-1 is required in order for intestinal ILC3s to produce large amounts of the protective effector cytokine IL-22 early in the course of infection. On a molecular level, our data indicate that IRF-1 controls ILC3 numbers and their activation by direct transcriptional regulation of the IL-12Rβ1 chain, thereby allowing ILCs to physiologically respond to IL-23 stimulation.
MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis
Authors: Takahashi Hiroki , Yang Ge , Yoneshiro Takeshi , Abe Yohei , Ito Ryo , Yang Chaoran , Nakazono Junna , Okamoto-Katsuyama Mayumi , Uchida Aoi , Arai Makoto , Jin Hitomi , Choi Hyunmi , Tumenjargal Myagmar , Xie Shiyu , Zhang Ji , Sagae Hina , Zhao Yanan , Yamaguchi Rei , Nomura Yu , Shimizu Yuichi , Yamada Kaito , Yasuda Satoshi , Kimura Hiroshi , Tanaka Toshiya , Wada Youichiro , Kodama Tatsuhiko , Aburatani Hiroyuki , Zhu Min-Sheng , Inagaki Takeshi , Osborne Timothy F. , Kawamura Takeshi , Ishihama Yasushi , Matsumura Yoshihiro , Sakai Juro 
Published: September 2022

AbstractProtein kinase A promotes beige adipogenesis downstream from β-adrenergic receptor signaling by phosphorylating proteins, including histone H3 lysine 9 (H3K9) demethylase JMJD1A. To ensure homeostasis, this process needs to be reversible however, this step is not well understood. We show that myosin phosphatase target subunit 1- protein phosphatase 1β (MYPT1-PP1β) phosphatase activity is inhibited via PKA-dependent phosphorylation, which increases phosphorylated JMJD1A and beige adipogenesis. Mechanistically, MYPT1-PP1β depletion results in JMJD1A-mediated H3K9 demethylation and activation of the Ucp1 enhancer/promoter regions. Interestingly, MYPT1-PP1β also dephosphorylates myosin light chain which regulates actomyosin tension-mediated activation of YAP/TAZ which directly stimulates Ucp1 gene expression. Pre-adipocyte specific Mypt1 deficiency increases cold tolerance with higher Ucp1 levels in subcutaneous white adipose tissues compared to control mice, confirming this regulatory mechanism in vivo. Thus, we have uncovered regulatory cross-talk involved in beige adipogenesis that coordinates epigenetic regulation with direct activation of the mechano-sensitive YAP/TAZ transcriptional co-activators.
Multicolor ultralong phosphorescence from perovskite-like octahedral α-AlF3
Authors: Cao Peisheng , Zheng Haoyue , Wu Peng 
Published: September 2022

AbstractDesigning organic fluorescent and phosphorescent materials based on various core fluorophore has gained great attention, but it is unclear whether similar luminescent units exist for inorganic materials. Inspired by the BX6 octahedral structure of luminescent metal halide perovskites (MHP), here we propose that the BX6 octahedron may be a core structure for luminescent inorganic materials. In this regard, excitation-dependent color-tunable phosphorescence is discovered from α-AlF3 featuring AlF6 octahedron. Through further exploration of the BX6 unit by altering the dimension and changing the center metal (B) and ligand (X), luminescence from KAlF4, (NH4)3AlF6, AlCl3, Al(OH)3, Ga2O3, InCl3, and CdCl2 are also discovered. The phosphorescence of α-AlF3 can be ascribed to clusterization-triggered emission, i.e., weak through space interaction of the n electrons of F atoms bring close proximity in the AlF6 octahedra (inter/intra). These discoveries will deepen the understanding and contribute to further development of BX6 octahedron-based luminescent materials.
High PSQI score is associated with the development of dyskinesia in Parkinson’s disease
Authors: Tang Xiaohui , Yang Jingyun , Zhu Yining , Gong Haiyan , Sun Hui , Chen Fan , Guan Qiang , Yu Lijia , Wang Weijia , Zhang Zengping , Li Li , Ma Guozhao , Wang Xijin 
Published: September 2022

AbstractDyskinesia is one of the most disabling motor complications in Parkinson’s Disease (PD). Sleep is crucial to keep neural circuit homeostasis, and PD patients often suffer from sleep disturbance. However, few prospective studies have been conducted to investigate the association of sleep quality with dyskinesia in PD. The objective of the current study is to investigate the association between sleep quality and dyskinesia and build a prediction model for dyskinesia in PD. We prospectively followed a group of PD patients without dyskinesia at baseline for a maximum of 36 months. Univariable and multivariable Cox regression with stepwise variable selection was used to investigate risk factors for dyskinesia. The performance of the model was assessed by the time-dependent area under the receiver-operating characteristic curve (AUC). At the end of follow-up, 32.8% of patients developed dyskinesia. Patients with bad sleep quality had a significantly higher proportion of dyskinesia compared with those with good sleep quality (48.1% vs. 20.6%, p = 0.023). Multivariable Cox regression selected duration of PD, sleep quality, cognition, mood, and levodopa dose. Notably, high Pittsburgh sleep quality index (PSQI) score was independently associated with an increased risk of dyskinesia (HR = 2.96, 95% CI 1.05–8.35, p = 0.041). The model achieved a good discriminative ability, with the highest AUC being 0.83 at 35 months. Our results indicated that high PSQI score may increase the risk of developing dyskinesia in PD, implying that therapeutic intervention targeting improving sleep quality may be a promising approach to prevent or delay the development of dyskinesia in PD.
Influence of point-of-use dispensers on lead level assessment in drinking water of a lead pipe-free campus
Authors: Adhikari Saroj , Lin Yi-Pin , Ng Ding-Quan 
Published: September 2022

AbstractPoint-of-use (POU) dispensers, referring to those directly connected to the water supply lines, are widely used in public facilities such as schools and universities in Taiwan. These dispensers are equipped with filters that can remove contaminants, including heavy metals in drinking water. Assessment of water lead (Pb) levels rely heavily on sampling surveys that involve various sampling protocols. This study evaluated the effects of using first draw (FD), flush (FL) incorporating at least 20 s of flushing, and random daytime (RDT) sampling protocols on Pb level assessment in water samples collected from faucets and POU dispensers of a Pb pipe-free campus between March 2017 and July 2020. This was the first study to examine the influence of POU dispensers on different sampling protocols and their survey results. Pb levels in 19% of faucet and 11% of dispenser samples exceeded the Taiwan EPA standard of 10 μg/L. FL sampling produced the lowest Pb levels, followed by RDT and FD in the samples collected from faucets. Interestingly, all three sampling protocols exhibited similar Pb levels in samples collected from dispensers. Thus, any of the three sampling protocols can be employed to monitor Pb levels in water samples collected from dispensers.
Breaking the hard-to-abate bottleneck in China’s path to carbon neutrality with clean hydrogen
Authors: Yang Xi , Nielsen Chris P. , Song Shaojie , McElroy Michael B. 
Published: September 2022

AbstractCountries such as China are facing a bottleneck in their paths to carbon neutrality: abating emissions in heavy industries and heavy-duty transport. There are few in-depth studies of the prospective role for clean hydrogen in these ‘hard-to-abate’ (HTA) sectors. Here we carry out an integrated dynamic least-cost modelling analysis. Results show that, first, clean hydrogen can be both a major energy carrier and feedstock that can significantly reduce carbon emissions of heavy industry. It can also fuel up to 50% of China’s heavy-duty truck and bus fleets by 2060 and significant shares of shipping. Second, a realistic clean hydrogen scenario that reaches 65.7 Mt of production in 2060 could avoid US$1.72 trillion of new investment compared with a no-hydrogen scenario. This study provides evidence of the value of clean hydrogen in HTA sectors for China and countries facing similar challenges in reducing emissions to achieve net-zero goals.
The NALCN channel regulates metastasis and nonmalignant cell dissemination
Authors: Rahrmann Eric P. , Shorthouse David , Jassim Amir , Hu Linda P. , Ortiz Mariaestela , Mahler-Araujo Betania , Vogel Peter , Paez-Ribes Marta , Fatemi Atefeh , Hannon Gregory J. , Iyer Radhika , Blundon Jay A. , Lourenço Filipe C. , Kay Jonathan , Nazarian Rosalynn M. , Hall Benjamin A. , Zakharenko Stanislav S. , Winton Douglas J. , Zhu Liqin , Gilbertson Richard J. 
Published: September 2022

AbstractWe identify the sodium leak channel non-selective protein (NALCN) as a key regulator of cancer metastasis and nonmalignant cell dissemination. Among 10,022 human cancers, NALCN loss-of-function mutations were enriched in gastric and colorectal cancers. Deletion of Nalcn from gastric, intestinal or pancreatic adenocarcinomas in mice did not alter tumor incidence, but markedly increased the number of circulating tumor cells (CTCs) and metastases. Treatment of these mice with gadolinium—a NALCN channel blocker—similarly increased CTCs and metastases. Deletion of Nalcn from mice that lacked oncogenic mutations and never developed cancer caused shedding of epithelial cells into the blood at levels equivalent to those seen in tumor-bearing animals. These cells trafficked to distant organs to form normal structures including lung epithelium, and kidney glomeruli and tubules. Thus, NALCN regulates cell shedding from solid tissues independent of cancer, divorcing this process from tumorigenesis and unmasking a potential new target for antimetastatic therapies.
Colitis-associated intestinal microbiota regulates brain glycine and host behavior in mice
Authors: Morozova Maryana V. , Borisova Mariya A. , Snytnikova Olga A. , Achasova Kseniya M. , Litvinova Ekaterina A. , Tsentalovich Yuri P. , Kozhevnikova Elena N. 
Published: September 2022

AbstractInflammatory bowel diseases (IBD) are chronic and relapsing inflammatory disorders of the gastrointestinal tract with complex etiology and no strategies for complete cure. IBD are often complicated by mental disorders like anxiety and depression, indicating substantial shifts in the microbiota gut-brain axis. However, the mechanisms connecting IBD to mental diseases are still under debate. Here we useMuc2knockout mouse model of chronic colitis to uncouple the effects of the intestinal microbiota on host behavior from chronic inflammation in the gut.Muc2knockout male mice exhibit high exploratory activity, reduced anxiety-related behaviors, impaired sensorimotor gating, and altered social preference towards males and females. Microbial transfer to wild-type mice via littermate co-housing shows that colitis-associated microbiota rather than inflammation per se defines behavioral features inMuc2colitis model. Metagenomic profiling and combination of antibiotic treatments revealed that bacterial speciesAkkermansia muciniphilais associated with the behavioral phenotype in mutants, and that its intestinal abundance correlates with social preference towards males. Metabolomic analysis together with pharmacological inhibition of Gly and NMDA receptors helped us to determine that brain glycine is responsible for the behavioral phenotype inMuc2mice. Blood and brain metabolic profiles suggest that microbiota-dependent changes in choline metabolism might be involved in regulation of central glycine neurotransmission. Taken together, our data demonstrates that colitis-associated microbiota controls anxiety, sensorimotor gating and social behavior via metabolic regulation of the brain glycinergic system, providing new venues to combat neurological complications of IBD.
A preclinical model of cutaneous melanoma based on reconstructed human epidermis
Authors: Leikeim Anna , Wußmann Maximiliane , Schmidt Freia F. , Neto Nuno G. B. , Benz Franziska , Tiltmann Kendra , Junger Corinna , Monaghan Michael G. , Schilling Bastian , Groeber-Becker Florian K. 
Published: September 2022

AbstractMalignant melanoma is among the tumor entities with the highest increase of incidence worldwide. To elucidate melanoma progression and develop new effective therapies, rodent models are commonly used. While these do not adequately reflect human physiology, two-dimensional cell cultures lack crucial elements of the tumor microenvironment. To address this shortcoming, we have developed a melanoma skin equivalent based on an open-source epidermal model. Melanoma cell lines with different driver mutations were incorporated into these models forming distinguishable tumor aggregates within a stratified epidermis. Although barrier properties of the skin equivalents were not affected by incorporation of melanoma cells, their presence resulted in a higher metabolic activity indicated by an increased glucose consumption. Furthermore, we re-isolated single cells from the models to characterize the proliferation state within the respective model. The applicability of our model for tumor therapeutics was demonstrated by treatment with a commonly used v-raf murine sarcoma viral oncogene homolog B (BRAF) inhibitor vemurafenib. This selective BRAF inhibitor successfully reduced tumor growth in the models harboring BRAF-mutated melanoma cells. Hence, our model is a promising tool to investigate melanoma development and as a preclinical model for drug discovery.
Misperceptions about out-partisans’ democratic values may erode democracy
Authors: Pasek Michael H. , Ankori-Karlinsky Lee-Or , Levy-Vene Alex , Moore-Berg Samantha L. 
Published: September 2022

AbstractTwo studies (one preregistered) of Americans (N = 2200) drawn from a nationally representative panel show that both Democrats and Republicans personally value core democratic characteristics, such as free and fair elections, but severely underestimate opposing party members’ support for those same characteristics. Democrats estimate that the average Democrat values democratic characteristics 56% (in Study 1) and 77% (in Study 2) more than the average Republican. In a mirror image, Republicans estimate that the average Republican values democratic characteristics 82% (in Study 1) and 88% (in Study 2) more than the average Democrat. In turn, the tendency to believe that political ingroup members value democratic characteristics more than political outgroup members is associated with support for anti-democratic practices, especially among Republicans. Results suggest biased and inaccurate intergroup perceptions may contribute to democratic erosion in the United States.
Morphotype broadening of the grapevine (Vitis vinifera L.) from Oxus civilization 4000 BP, Central Asia
Authors: Chen Guanhan , Zhou Xinying , Khasannov Mutalibjon , Spengler Robert N. , Ma Jian , Annaev Tukhtash , Kambarov Nasibillo , Maksudov Farhod , Wang Jianxin , Askarov Akhmadali , Li Xiaoqiang 
Published: September 2022

AbstractThe region of Transoxiana underwent an early agricultural-demographic transition leading to the earliest proto-urban centers in Central Asia. The agronomic details of this cultural shift are still poorly studied, especially regarding the role that long-generation perennials, such as grapes, played in the cultivation system. In this paper, we present directly dated remains of grape pips from the early urban centers of Sapalli and Djarkutan, in south Uzbekistan. We also present linear morphometric data, which illustrate a considerable range of variation under cultivation that we divide into four distinct morphotypes according to pip shape. While some of the pips in these two assemblages morphologically fall within the range of wild forms, others more closely resemble modern domesticated populations. Most of the specimens measure along a gradient between the two poles, showing a mixed combination of domesticated and wild features. We also point out that the seeds recovered from the Djarkutan temple were, on average, larger and contained more affinity towards domesticated forms than those from domestic contexts. The potential preference of morphotypes seems to suggest that there were recognized different varieties that local cultivators might aware and possibly propagating asexually.
The impact of brain lesion characteristics and the corticospinal tract wiring on mirror movements in unilateral cerebral palsy
Authors: Simon-Martinez Cristina , Decraene Lisa , Zielinski Ingar , Hoare Brian , Williams Jacqueline , Mailleux Lisa , Steenbergen Bert , Ortibus Els , Feys Hilde , Klingels Katrijn 
Published: September 2022

AbstractMirror movements (MM) influence bimanual performance in children with unilateral cerebral palsy (uCP). Whilst MM are related to brain lesion characteristics and the corticospinal tract (CST) wiring pattern, the combined impact of these neurological factors remains unknown. Forty-nine children with uCP (mean age 10y6mo) performed a repetitive squeezing task to quantify similarity (MM-similarity) and strength (MM-intensity) of the MM activity. We used MRI data to evaluate lesion type (periventricular white matter, N = 30; cortico-subcortical, N = 19), extent of ipsilesional damage, presence of bilateral lesions, and damage to basal ganglia, thalamus and corpus callosum. The CST wiring was assessed with Transcranial Magnetic Stimulation (17 CSTcontralateral, 16 CSTipsilateral, 16 CSTbilateral). Data was analyzed with regression analyses. In the more-affected hand, MM-similarity and intensity were higher with CSTbilateral/ipsilateral. In the less-affected hand, MM-similarity was higher in children with (1) CSTcontra with CSC lesions, (2) CSTbilat/ipsi with PVL lesions and (3) CSTbilat/ipsi with unilateralized lesions. MM-intensity was higher with larger damage to the corpus callosum and unilateral lesions. A complex combination of neurological factors influences MM characteristics, and the mechanisms differ between hands.
Predicting the in-game status in soccer with machine learning using spatiotemporal player tracking data
Authors: Lang Steffen , Wild Raphael , Isenko Alexander , Link Daniel 
Published: September 2022

AbstractAn important structuring feature of a soccer match is the in-game status, whether a match is interrupted or in play. This is necessary to calculate performance indicators relative to the effective playing time or to find standard situations, ball actions, and other tactical structures in spatiotemporal data. Our study explores the extent to which the in-game status can be determined using time-continuous player positions. Therefore, to determine the in-game status we tested four established machine learning methods: logistic regression, decision trees, random forests, and AdaBoost. The models were trained and evaluated using spatiotemporal data and manually annotated in-game status of 102 matches in the German Bundesliga. Results show up to 92% accuracy in predicting the in-game status in previously unknown matches on frame level. The best performing method, AdaBoost, shows 81% precision for detecting stoppages (longer than 2 s). The absolute time shift error at the start was ≤ 2 s for 77% and 81% at the end for all correctly predicted stoppages. The mean error of the in-game total distance covered per player per match using the AdaBoost in-game status prediction was − 102 ± 273 m, which is 1.3% of the mean value of this performance indicator (7939 m). Conclusively, the prediction quality of our model is high enough to provide merit for performance diagnostics when teams have access to player positions (e.g., from GPS/LPM systems) but no human-annotated in-game status and/or ball position data, such as in amateur or youth soccer.
Assessing a megadiverse but poorly known community of fishes in a tropical mangrove estuary through environmental DNA (eDNA) metabarcoding
Authors: Zainal Abidin Danial Hariz , Mohd. Nor Siti Azizah , Lavoué Sébastien , A. Rahim Masazurah , Mohammed Akib Noor Adelyna 
Published: September 2022

AbstractBiodiversity surveys are crucial for monitoring the status of threatened aquatic ecosystems, such as tropical estuaries and mangroves. Conventional monitoring methods are intrusive, time-consuming, substantially expensive, and often provide only rough estimates in complex habitats. An advanced monitoring approach, environmental DNA (eDNA) metabarcoding, is promising, although only few applications in tropical mangrove estuaries have been reported. In this study, we explore the advantages and limitations of an eDNA metabarcoding survey on the fish community of the Merbok Estuary (Peninsular Malaysia). COI and 12S eDNA metabarcoding assays collectively detected 178 species from 127 genera, 68 families, and 25 orders. Using this approach, significantly more species have been detected in the Merbok Estuary over the past decade (2010–2019) than in conventional surveys, including several species of conservation importance. However, we highlight three limitations: (1) in the absence of a comprehensive reference database the identities of several species are unresolved; (2) some of the previously documented specimen-based diversity was not captured by the current method, perhaps as a consequence of PCR primer specificity, and (3) the detection of non-resident species—stenohaline freshwater taxa (e.g., cyprinids, channids, osphronemids) and marine coral reef taxa (e.g., holocentrids, some syngnathids and sharks), not known to frequent estuaries, leading to the supposition that their DNA have drifted into the estuary through water movements. The community analysis revealed that fish diversity along the Merbok Estuary is not homogenous, with the upstream more diverse than further downstream. This could be due to the different landscapes or degree of anthropogenic influences along the estuary. In summary, we demonstrated the practicality of eDNA metabarcoding in assessing fish community and structure within a complex and rich tropical environment within a short sampling period. However, some limitations need to be considered and addressed to fully exploit the efficacy of this approach.