bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential expression of immune-regulatory proteins C5AR1, CLEC4A and NLRP3 on peripheral blood mononuclear cells in early-stage non-small cell lung cancer patients
Authors: Pakvisal Nussara , Kongkavitoon Pornrat , Sathitruangsak Chirawadee , Pornpattanarak Nopporn , Boonsirikamchai Piyaporn , Ouwongprayoon Pongsakorn , Aporntewan Chatchawit , Chantranuwatana Poonchavist , Mutirangura Apiwat , Vinayanuwattikun Chanida 
Published: November 2022

AbstractChanges in gene expression profiling of peripheral blood mononuclear cells (PBMC) appear to represent the host’s response to the cancer cells via paracrine signaling. We speculated that protein expression on circulating T-lymphocytes represent T-lymphocyte trafficking before infiltration into the tumor microenvironment. The possibility of using protein expression on circulating T-lymphocytes as a biomarker to discriminate early-stage non-small cell lung cancer (NSCLC) was explored. Four independent PBMC gene expression microarray datasets (GSE12771, GSE13255, GSE20189 and GSE3934) were analyzed. We selected C5AR1, CLEC4A and NLRP3 based on their significant protein expression in tumor-infiltrating lymphocytes, but not in normal lymphoid tissue. A validation study using automated flow cytometry was conducted in 141 study participants including 76 treatment-naive early-stage non-small cell lung cancer patients (NSCLC), 12 individuals with non-malignant pulmonary diseases, and 53 healthy individuals. Median ratios of C5AR1, CLEC4A and NLRP3 specific antibody staining to CD3 positive cells in early-stage NSCLC patients compared to healthy controls were 0.014 [0–0.37] vs. 0.01 [0–0.07,p = 0.13], 0.03 [0–0.87] vs. 0.02 [0–0.13,p = 0.10] and 0.19 [0–0.60] vs. 0.09 [0.02–0.31,p < 0.0001], respectively. Median fluorescence intensity (MFI) of CD3+C5AR1+, CD3+CLEC4A+and CD3+NLRP3+expression in early-stage NSCLC patients compared to healthy volunteers was 185 [64.2–4801] vs. 107.5 [27–229,p < 0.0001], 91.2 [42.4–2355] vs. 71.25 [46.2–103,p = 0.0005], and 1585 [478–5224] vs. 758.5 [318–1976,p < 0.0001], respectively. NLRP3:CD3 ratio, CD3+C5AR1+, CD3+CLEC4A+and CD3+NLRP3+MFI were significantly higher in early-stage NSCLC than healthy volunteers with an area under the ROC curve of 0.69–0.76. The CD3+NLRP3+MFI provided the most distinguishable expression at 71.5% sensitivity and 70% specificity. Furthermore, CD3+NLRP3+MFI potentially discriminated between early-stage NSCLC from malignant-mimic inflammation and infection pulmonary disease. Further validation in various pulmonary inflammatory disease might be warranted. Our proof-of-principle findings strengthen the hypothesis that malignancies generate distinctive protein expression fingerprints on circulating T-lymphocytes.
An innovative approach for the evaluation of prolonged disorders of consciousness using NF-L and GFAP biomarkers: a pivotal study
Authors: Coppola L. , Mirabelli P. , Baldi D. , Smaldone G. , Estraneo A. , Soddu A. , Grimaldi A. M. , Mele G. , Salvatore Marco , Cavaliere Carlo 
Published: November 2022

AbstractBehavioral assessments during the clinical evaluation in prolonged disorders of consciousness patients could be not sufficient for a correct diagnosis and prognostication. To this aim, we used an innovative approach, involving the ultra-sensitive determination of biological markers, correlating them with imaging parameters to investigate the prolonged disorders of consciousness (pDoC).We assessed the serum concentration of neurofilament light chain(NF-L) and glial fibrillary acidic protein (GFAP) in pDoC (n = 16), and healthy controls (HC, n = 6) as well as several clinical imaging parameters such as Fractional Anisotropy (FA), Whole Brain SUV, and White Matter Hyperintensities volumes (WMH) using PET-MRI acquisition. As for differential diagnosis task, only the imaging WMH volume was able to discriminate between vegetative state/unresponsive wakefulness syndrome (VS/UWS), and minimally conscious state (MCS) patients (p-value < 0.01), while all selected markers (both imaging and in vitro) were able to differentiate between pDoC patients and HC. At subject level, serum NF-L concentrations significantly differ according to clinical progression and consciousness recovery (p-value < 0.01), highlighting a potential play for the longitudinal management of these patients.
Facile synthesis of polyoxometalate supported on magnetic graphene oxide as a hybrid catalyst for efficient oxidation of aldehydes
Authors: Zarnegaryan Ali 
Published: November 2022

AbstractIn the present study, Anderson-type polyoxometalate [N(C4H9)4] [FeMo6O18(OH)6] (FeMo6) was immobilized on amino-modified magnetic graphene oxide and employed as a new hybrid catalyst in oxidation of aldehydes to carboxylic acids. The synthesized hybrid catalyst Fe3O4/GO/[FeMo6] was characterized using thermogravimetric analysis (TGA), scanning electron microscopies (SEM), Fourier transform infrared (FT-IR), vibrating sample magnetometry (VSM), energy-dispersive X‐ray analysis (EDX), Raman spectroscopy and inductively coupled plasma atomic emission spectroscopy (ICP-OES). The results indicated that our catalyst was quite active in oxidizing the aldehydes to their corresponding carboxylic acids in the presence of hydrogen peroxide. The synthesized catalyst can be easily separated from the reaction medium and reused for six consecutive runs without a significant reduction in reaction efficiency.
A closed form shape function describing 3D settlement field around a deep excavation in sand
Authors: Russo Gianpiero , Nicotera Marco Valerio 
Published: November 2022

AbstractSoil movements produced around deep excavations are one of the main issues to be addressed during design and construction of underground structures in urban environment. Several methods to predict ground movements are currently available. Semi-empirical methods correlate displacements and simple geometrical features of the excavation; these methods predict separately transversal and longitudinal settlement troughs or at least provide a conservative envelope of them and they are mainly based on empirical data of excavations in clay. Numerical methods based on the solutions of FEM or DFM models usually provide prediction of the green field subsidence. In this paper existing 2D semi-empirical methods are first shortly reviewed. Some of these methods are then modified and combined into a new 3D analytical description of the subsidence trough around a deep excavation. The proposed two variable subsidence function depends on several parameters: four independent parameters defining the overall shape and the maximum settlement acting as a scale parameter. Settlement fields reproduced by a 3D FEM nonlinear parametric study of deep excavations inspired by real case studies are thus presented and discussed. The newly proposed 3D analytical description is applied to fit the FEM results and some fundamental relationships among geometrical features of the excavation pit and the 3D shape function parameters are identified. These relationships are validated via the application to three case studies of deep excavations in sand where the subsidence was controlled by the deformation of the retaining structures finding a satisfactory and encouraging agreement.
Antibiotic properties of Satureja montana L. hydrolate in bacteria and fungus of clinical interest and its impact in non-target environmental microorganisms
Authors: Pino-Otín María Rosa , Gan Cristina , Terrado Eva , Sanz María Angeles , Ballestero Diego , Langa Elisa 
Published: November 2022

AbstractThe aim of this study was to analyse the microbicidal and microbiostatic activity of S. montana hydrolate L., the water-soluble fraction of the hydro-distillation process used to obtain the essential oil, on 14 Gram-positive and Gram-negative bacteria and a fungus of clinical interest. To consider whether this hydrolate is a more environmentally friendly alternative to traditional antibiotics, its effect on non-target microorganisms in the aquatic and terrestrial environment was analysed using natural soil and river microorganism communities, characterized through 16S rRNA gene sequencing. Results showed that S. montana hydrolate was especially effective (25% v/v concentration) against Pasteurella aerogenes, Streptococcus agalactiae and Acinetobacter baumannii (priority 1, WHO). It was also a microbicide for a further 7 bacterial strains and the fungus Candida albicans (50% v/v concentration). The river and soil communities exposed to the hydrolate showed a decrease in their growth, as well as a decrease in their ability to metabolize polymers and carbohydrates (soil microorganisms) and polymers, carboxylic and ketone acids (river microorganisms). Hydrolates could be an alternative to conventional antibiotics, but their impact on the environment must be taken into account.
Secure secondary utilization system of genomic data using quantum secure cloud
Authors: Fujiwara Mikio , Hashimoto Hiroki , Doi Kazuaki , Kujiraoka Mamiko , Tanizawa Yoshimichi , Ishida Yusuke , Sasaki Masahide , Nagasaki Masao 
Published: November 2022

AbstractSecure storage and secondary use of individual human genome data is increasingly important for genome research and personalized medicine. Currently, it is necessary to store the whole genome sequencing information (FASTQ data), which enables detections of de novo mutations and structural variations in the analysis of hereditary diseases and cancer. Furthermore, bioinformatics tools to analyze FASTQ data are frequently updated to improve the precision and recall of detected variants. However, existing secure secondary use of data, such as multi-party computation or homomorphic encryption, can handle only a limited algorithms and usually requires huge computational resources. Here, we developed a high-performance one-stop system for large-scale genome data analysis with secure secondary use of the data by the data owner and multiple users with different levels of data access control. Our quantum secure cloud system is a distributed secure genomic data analysis system (DSGD) with a “trusted server” built on a quantum secure cloud, the information-theoretically secure Tokyo QKD Network. The trusted server will be capable of deploying and running a variety of sequencing analysis hardware, such as GPUs and FPGAs, as well as CPU-based software. We demonstrated that DSGD achieved comparable throughput with and without encryption on the trusted server Therefore, our system is ready to be installed at research institutes and hospitals that make diagnoses based on whole genome sequencing on a daily basis.
Ultrasound performance using the EU-TIRADS score in the diagnosis of thyroid cancer in Congolese hospitals
Authors: Bukasa-Kakamba John , Bayauli Pascal , Sabbah Nadia , Bidingija Joseph , Atoot Ali , Mbunga Branly , Nkodila Aliocha , Atoot Adam , Bangolo Ayrton Ilolo , M’Buyamba-Kabangu Jean Rene 
Published: November 2022

AbstractThe thyroid imaging reporting and data systems by the European Thyroid Association (EU-TIRADS) has been widely used in malignancy risk stratification of thyroid nodules. However, there is a paucity of data in developing countries, especially in Africa, to validate the use of this scoring system. The aim of the study was to assess the diagnostic value of the EU-TIRADS score in Congolese hospitals, using pathological examination after surgery as the gold standard in Congolese hospitals. This retrospective and analytical study examined clinical, ultrasound and pathological data of 549 patients aged 45 ± 14 years, including 468 females (85.2%), operated for thyroid nodule between January 2005 and January 2019. In the present study, only the highest graded nodule according to the EU-TIRADS score in each patient was taken into account for the statistical analyses. So 549 nodules were considered. Nodules classified EU-TIRADS 2 and 3 on the one hand, and, on the other hand, 4 and 5, were considered respectively at low and high risk of malignancy. The sensitivity and specificity of the EU-TIRADS score were calculated. The significance level was set at 5%. Of all patients, 21.7% had malignant nodules. They made 48.4% of the nodules in patients younger than and at 20 years old, and 31.1% in those aged 60 or over. Malignant nodules were more frequent in men than in women (30.9% vs. 20.1%; p = 0.024). Papillary carcinoma (67.2%) and follicular carcinoma (21.8%) were the main types. The malignancy rate was 39.7% and 1.5% among nodules rated EU-TIRADS 4 and 5, and those with EU-TIRADS score 2 and 3, respectively (p < 0.001). The EU-TIRADS score had a sensitivity of 96.6% and a specificity of 59.3%. The ROC curve indicated an area under the curve of 0.862. In a low-income country, a well performed thyroid ultrasound, using the EU-TIRADS score, could be an important tool in the selection of thyroid nodules suspected of malignancy and requiring histopathological examination in the Congolese hospital setting.Trial registration: The research protocol had obtained the favorable opinion of the DRC national health ethics committee no. 197/CNES/BN/PMMF/2020. The data was collected and analyzed anonymously.
Optimization of oviposition trap settings to monitor populations of Aedes mosquitoes, vectors of arboviruses in La Reunion
Authors: Brouazin Ronan , Claudel Iris , Lancelot Renaud , Dupuy Guillaume , Gouagna Louis-Clément , Dupraz Marlène , Baldet Thierry , Bouyer Jérémy 
Published: November 2022

AbstractSeveral dengue epidemics recently occurred in La Reunion, an island harboring two dengue viruses (DVs) vectors: Aedes albopictus, and Ae. aegypti, the former being the main local DV vector. Aedes aegypti shows a peculiar ecology, compared to other tropical populations of the same species. This study aimed to provide researchers and public-health users with locally validated oviposition traps (ovitraps) to monitor Aedes populations. A field experiment was performed in Saint-Joseph to assess the effect of different settings on the detection probability and apparent density of Aedes mosquitoes. Black plastic ovitraps were identified as the best choice. Vacoa trees (Pandanus utilis) were the only observed breeding sites for Ae. aegypti, shared with Ae. albopictus. They were the experimental units in a Latin square design with three factors: trap position in the trees (ground vs canopy), oviposition surface in the trap (blotting paper vs. vacoa leaf), and addition of organic matter to the trap water. The latter factor was found unimportant. On the ground, Ae. aegypti eggs were only found with vacoa leaves as the oviposition surface. Their detection and apparent density increased when ovitraps were located in the tree canopy. The main factor for Ae. albopictus was the oviposition surface, with a preference for blotting paper. In all trap settings, their detection was close to 100%. Larval survival was lower for a high egg density, combined with blotting paper as the oviposition surface. When monitoring mixed Aedes populations in La Reunion, we recommend using black plastic ovitraps, placed at 1.50-to-2.00-m high in vacoa trees, with vacoa leaves as the oviposition surface.
A novel wrinkled-leaf sesame mutant as a potential edible leafy vegetable rich in nutrients
Authors: Liu Hongyan , Zhou Fang , Zhou Ting , Yang Yuanxiao , Zhao Yingzhong 
Published: November 2022

AbstractSesame (Sesamum indicum L.) is an ancient and globally important oil crop in the tropic and subtropic areas. Apart from being a good source of high-quality oil, sesame also represents a new source of edible leafy vegetables. However, data regarding the nutritional composition of the sesame leaves, especially their phytonutrient composition, are scarce. Previously we have developed a sesame mutant JQA with curly, wide, and thick leaves that are potentially used as a vegetable. The objective of this work was to gauge the nutrient contents in leaves of the JQA mutant by colorimetry methods. The sesame mutant JQA and its wild-type counterpart JQB were grown in the field, and leaf samples were collected at the flowering stage. Results showed that the sesame wrinkled leaves of JQA are a rich source of crude oil (5.33–6.38%), crude protein (3.14%), amino acids (> 18.6 mg/g), crude fiber (> 0.36%), cellulose or hemicellulose (> 21.4 mg/g), sugars (> 12.5 mg/g), vitamins, and flavones (> 63.2 mg/g). The wrinkled sesame leaves were high in unsaturated acid (32.0 mg/g), calcium (18.5 mg/g), potassium (16.1 mg/g), as well as vitamin B6 (24.5 mg/g), B2 (14.4 mg/g), C (1.7 mg/g) and D (1.3 mg/g) compared to other common green leafy vegetables. The fresh leaves had a mean total flavone content of 65.7 mg/g and can be consumed as fresh vegetables or preserved in a dry state. Collectively, the nutritional composition of the wrinkled leaf mutant JQA was ideal and thus had high RDIs (recommended daily intakes), suggesting that the wrinkled leaves are a rich source of nutrients and therefore suitable to be consumed as a new edible green vegetable.
The impact of Hounsfield unit-related variables on percutaneous nephrolithotomy outcomes
Authors: Moon Hyong Woo , Taeyb Mustafa , Park Yong Hyun , Bae Woong Jin , Ha U.-Syn , Hong Sung-Hoo , Lee Ji Youl , Kim Sae Woong , Cho Hyuk Jin 
Published: November 2022

AbstractWe aimed to identify the association between Hounsfield Unit(HU)-related variables and percutaneous nephrolithotomy (PCNL) outcomes. We enrolled patients with single renal stones (1–3 cm) who underwent single-tract PCNL between January 2014 and October 2019. Demographics and stone characteristics were retrospectively reviewed. Preoperative computerized tomography (CT) and follow-up CT within at least 3 months after PCNL were included in this analysis. Stone-free status was defined as residual stone measuring ≤ 2 mm within 3 months postoperatively. HU and cross-sectional area (CSA) were measured using the free-draw technique. We analyzed HU-related variables using logistic regression model for outcomes. Altogether, 188 out of 683 patients met the inclusion criteria. The stone-free rate (SFR) was 79.2%. There were no significant differences in age, sex, BMI, ASA class, laterality, pre-op shockwave lithotripsy, stone size, stone burden, skin-to-stone distance, and HU between the stone-free and remnant groups. CSA and HU/CSA in the stone-free and remnant groups were 94.5 ± 46.1 and 128.3 ± 98.5 (p = 0.043) and 10.1 ± 5.6 and 7.3 ± 3.4 (p = 0.001), respectively. Multivariate logistic regression analysis revealed that pelvis, ureteropelvic junction stones, and HU/CSA were independent predictors of SFR. HU did not affect PCNL outcomes. We believe that HU/CSA could be used for determining stone treatment plans and predicting outcomes.
Over-the-Counter Hearing Aids: What the Practicing Audiologist Needs to Know
Authors: Coco Laura 
Published: November 2022

Purpose:
The purpose of this clinical focus article is to provide an overview of the United States Food and Drug Administration's (FDA) over-the-counter (OTC) hearing aid rule, and discuss opportunities for clinicians to support individuals who self-direct their hearing care.

Conclusions:
The introduction of OTC hearing aids has implications for potentially all U.S. audiologists, even those who do not plan to directly interact with the devices. This clinical focus article provides audiologists with an overview of the historical background on OTC hearing aids and summarizes the FDA's rule. It also introduces recent studies that explore dimensions of patient outcomes with self-fitting hearing aids, including satisfaction with service delivery. In addition, readers are provided with clinical applications, or ways in which they can support patients who pursue OTC hearing aids, including by providing support services that help patients navigate the emotional and psychosocial aspects of hearing loss.
A new four‐row integral transposition method of stator windings and circulating current loss reduction in large turbo generators
Authors: Zhang Linlin , Liang Yanping 
Published: November 2022

AbstractTransposition of stator windings for large turbo generators is a key technology. At present, the stator windings with four‐row strands for large turbo generators are usually transposed on both sides of the slot centreline and connected at the end by the joint sleeve. The different transposition methods of strands have an important effect on strand currents, circulating currents, and circulating current losses. A new four‐row integral transposition method is proposed in this study. Several transposition methods based on the four‐row integral transposition method are compared with the double Roebel transposition methods using 3‐D time‐harmonic field. The 3‐D model without the end and with the end are both calculated. The results show that the four‐row integral transposition method has an obvious advantage in reducing circulating current loss. Finally, a 2‐D finite element model of transient magnetic field is calculated and its calculation results further support for the effectiveness of the proposed transposition method. The proposed transposition method has a great application value, which can reduce additional loss, avoid partial overheating and short circuit fault of stator windings in large turbo generators.
A current sensorless control method for multi‐level active front‐end rectifiers with LCL filter
Authors: Bayhan Sertac , Komurcugil Hasan 
Published: November 2022

AbstractThis paper presents a current sensorless control strategy for a three‐phase multi‐level active front‐end rectifier with an LCL filter. The proposed control strategy is based on the mathematical model of the system. Opposed to the existing control methods that measure three‐phase currents as well as three‐phase voltages, the proposed control eliminates the current measurement requirement which leads to cost reduction. Moreover, unlike the existing current control methods that use two proportional‐integral (PI) controllers, the proposed current control does not use any PI controller in the current loop. The elimination of two PI controllers simplifies the controller design. The resonance damping requirement arising due to the LCL filter is tackled by active damping that is realized by using filter capacitor voltage feedback. The steady‐state and dynamic performances of the proposed control strategy are investigated experimentally under balanced, unbalanced and distorted grid conditions.
Optimal identification of model parameters for PEMFCs using neoteric metaheuristic methods
Authors: Diab Ahmed A. Zaki , Abdelhamid Ashraf M. 
Published: November 2022

AbstractIn this paper, the neoteric metaheuristic methods of artificial ecosystem‐based optimization (AEO), Coot Bird‐based optimization (COOT), Equilibrium optimizer (EQO), Runge–Kutta Method (RUN), Hunger games search (HGS), and Weighted Mean of Vectors (INFO) have been applied and evaluated to discover a preferable estimation for the Proton Exchange Membrane Fuel Cells (PEMFCs) model. The validation of the applied methods has been done for valuing the model parameters of BCS 500W‐PEM, 500W‐SR‐12PEM, and 250W‐stack. The objective function has been formulated as the sum of square errors (SSEs) between the measured and estimated data. MATLAB has been used for the verification of the optimization techniques. The results show that for BCS 500W‐PEM: (1) the six algorithms can accurately solve the problem of the FC parameter assessment; (2) there are small distinguished between the six algorithms concerning their best value of the objective function; this distinction between the best and worst techniques is 2.4 × 10−9; (3) the best algorithm is INFO with 0.011556306 while the worst algorithm is RUN with 0.011556308; (4) statistical results prove that the six algorithms have the tracking efficiencies of 99.99961329%, 99.97114568%, 99.83529736%, 100%, 99.89195368%, and 99.90200833% for AEO, COOT, EQO, INFO, HGS, and RUN, respectively based on 30 individual runs.