bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of different mosquito collection methods on indicators of Anopheles malaria vectors in Uganda
Authors: Mawejje Henry , Asiimwe Jackson R , Kyagamba Patrick , Kamya Moses R , Rosenthal Philip J , Lines Jo , Dorsey Grant , Staedke Sarah G 
Published: October 2022

Abstract
Background:
Methods used to sample mosquitoes are important to consider when estimating entomologic metrics. Human landing catches (HLCs) are considered the gold standard for collecting malaria vectors. However, HLCs are labor intensive, can expose collectors to transmission risk, and are difficult to implement at scale. This study compared alternative methods to HLCs for collecting Anopheles mosquitoes in eastern Uganda.
Methods:
Between June and November 2021, mosquitoes were collected from randomly selected households in three parishes in Tororo and Busia districts. Mosquitoes were collected indoors and outdoors using HLCs in 16 households every 4 weeks. Additional collections were done indoors with prokopack aspirators, and outdoors with pit traps, in these 16 households every 2 weeks. CDC light trap collections were done indoors in 80 households every 4 weeks. Female Anopheles mosquitoes were identified morphologically and An. gambiae s.l. were speciated using PCR. Plasmodium falciparumsporozoite testing was done with ELISA.
Results:
Overall, 4,891 female Anopheles were collected, including 3,318 indoors and 1,573 outdoors. Compared to indoor HLCs, vector density (mosquitoes per unit collection) was lower using CDC light traps (4.24 vs 2.96, density ratio [DR] 0.70, 95% CIs 0.63 – 0.77, p<0.001) and prokopacks (4.24 vs 1.82, DR 0.43, 95% CIs 0.37 – 0.49, p<0.001). Sporozoite rates were similar between indoor methods, although precision was limited. Compared to outdoor HLCs, vector density was higher using pit trap collections (3.53 vs 6.43, DR 1.82, 95% CIs 1.61 – 2.05, p<0.001), while the sporozoite rate was lower (0.018 vs 0.004, rate ratio [RR] 0.23, 95% CIs 0.07 – 0.75, p=0.008). The main differences between collection methods were: (1) An. funestus were over-represented in the prokopack collections (75.0%) compared to the indoor HLCs (25.8%), and (2) An. arabiensis were over-represented in the pit trap collections (84.3%) compared to the outdoor HLCs (36.9%).
Conclusion:
In this setting, CDC light traps and prokopack aspirators are alternative methods for sampling mosquitoes indoors, each with advantages, but prokopacks may not provide an accurate sampling of mosquitoes responsible for malaria transmission. Pit traps could be a useful alternative for sampling outdoor resting mosquitoes, but accuracy of measures of transmission intensity may be limited.
The association between dietary magnesium intake and hemoglobin glycation index
Authors: Chen Juan , Lin Song , Wang Xingzhou , Wang Xiwei , Pengxia Gao 
Published: October 2022

Abstract
Objective
The data for the effect of dietary magnesium (Mg) on hemoglobin glycation index (HGI) is limited. Thus, this study aimed to examine the relationship between dietary Mg and HGI in the general population.
Methods
Our research used the data from the National Health and Nutrition Examination Survey from 2001 to 2002. The dietary intake of Mg was assessed by two 24-h dietary recalls. The predicted HbA1c was calculated based on fasting plasma glucose. Logistic regression and restricted cubic spline models were applied to assess the relationship between dietary Mg intake and HGI.
Results
We found a significant inverse association between dietary Mg intake and HGI (β = -0.00016, 95%CI: -0.0003, -0.00003, P = 0.019). Dose-response analyses revealed that HGI decreased with increasing intakes of Mg when reached the point above 412 mg/d. There was a linear dose-response relationship between dietary Mg intake and HGI in diabetic subjects, and there was an L-shape dose-response relationship in non-diabetic individuals.
Conclusion
Increasing the intake of Mg might protect from HGI. Further prospective studies are requested before dietary recommendations.
Biological properties of Staphylococcus virus ΦSA012 for phage therapy
Authors: Fujiki Jumpei , Nakamura Tomohiro , Nakamura Keisuke , Nishida Keita , Amano Yurika , Watanabe Yusaku , Gondaira Satoshi , Usui Masaru , Shimizu Masaru , Miyanaga Kazuhiko , Watanabe Shinya , Iwasaki Tomohito , Kiga Kotaro , Hanawa Tomoko , Higuchi Hidetoshi , Sawa Teiji , Tanji Yasunori , Tamura Yutaka , Cui Longzhu , Iwano Hidetomo 
Published: October 2022

Abstract
Staphylococcus virus ΦSA012 has a wide host range and efficient lytic activity. Here, we assessed the biological stability of ΦSA012 against temperature, freeze-thawing, and pH to clinically apply the phage. In addition, inoculation of ΦSA012 through i.p. and i.v. injections into mice revealed that phages were reached the limit of detection in serum and accumulated notably spleens without inflammation at 48 h post-inoculation. Furthermore, inoculation of ΦSA012 through s.c. injections in mice significantly induced IgG, which possesses neutralizing activity against ΦSA012 and other Staphylococcusviruses, ΦSA039 and ΦMR003, but not Pseudomonasviruses ΦS12-3 and ΦR18 or Escherichiaviruses T1, T4, and T7 in vitro. Immunoelectron microscopic analysis showed that purified anti-phage IgG recognizes the long-tail fiber of staphylococcus viruses. Although S. aureus inoculation resulted in a 25% survival rate in a mouse i.p. model, ΦSA012 inoculation (i.p.) improved the survival rate to 70%; however, the survival rate of ΦSA012-immunized mice decreased to less than non-immunized mice with phage i.v. injection at a MOI of 100. These results indicated that ΦSA012 possesses promise for use against staphylococcal infections but we should carefully address the appropriate dose and periods of phage administration. Our findings facilitate understandings of staphylococcus viruses for phage therapy.
Partial Domination of Network Models *
Authors: Zhang Shumin , Jia Tianxia , Zhao Conghui 
Published: October 2022

Abstract
Partial domination [8] is a theory first proposed in 2015 on the basis of domination theory, which has a wide range of research value. Let G = (V, E) be a graph and F be a family of graphs, a subset S ⊆ V is called an F-isolating set of G if G[V \NG[S]] does not contain F as a subgraph for all F ∈ F. If F = {K2}, S is an isolating set of G if G[V \NG[S]] does not contain K2. The isolation number of G is the minimum cardinality of an isolating set of G, denoted by ι(G). The hypercube network and n-star network are the basic models for interconnection networks, and they have many attractive topological properties. In this paper, we investigate the sharp bounds of the isolation numbers of the hypercube network Qn and n-star network Sn, and obtain (2n−1)\n ≤ ι(Qn) ≤ 2n−3 for any positive integer n ≥ 4 and (n·(n2−2)!)\2 ≤ ι(Sn) ≤ (n − 1)! for any positive integer n ≥ 2.
AMS subject classification: 05C05, 05C12, 05C76
Emergent Hybrid Surgical Approaches for Non-Dissecting Ruptured Kommerell's Aneurysm: A Case Series
Authors: Velandia-Sánchez Alejandro , Gómez-Galán Sebastián , Gallo-Bernal Sebastian , Polanía-Sandoval Camilo A. , Pineda-Rodríguez Ivonne G. , Florez-Amaya Paula , Sanabria-Arévalo Lina M. , Senosiain-González Julián , Barrera-Carvajal Juan G. , Umaña Juan P. , Camacho-Mackenzie Jaime 
Published: October 2022

Abstract
Background
Kommerell's diverticulum is a rare developmental abnormality of the aorta, associated in 20–60% of the cases with an aberrant subclavian artery. A Kommerell’s aneurysm is a saccular or fusiform dilatation that can be found in 3–8% of Kommerell’s diverticulum cases. A non-dissecting rupture rate of 6% has been reported. Because the patient's life is at risk, emergent surgical correction is usually granted. Different surgical interventions have been proposed, including open, endovascular, or hybrid approaches. However, evidence regarding the optimal surgical approach in the acute setting is scarce. In this case series, we aim to describe our surgical experience in the management of type-1 non-dissecting ruptured Kommerell's aneurysm.
Cases presentation:
From January 2005 to December 2020, three cases of type-1 non-dissecting ruptured Kommerell's aneurysm requiring emergent surgical repair were identified. The mean age was 66.67 ± 7.76 years, and 3/3 were male. The most common symptoms were atypical chest pain, dyspnea, and headache (2/3). The most frequent comorbidity was hypertension (2/3). The mean aneurysm’s diameter was 63.67 ± 5.69 mm. Frozen Elephant Trunk was the preferred surgical approach (2/3). The Non-Frozen Elephant Trunk patient underwent a hybrid procedure consisting of a carotid-subclavian bypass and a zone-2 stent graft deployment. We found a mean clamp time of 140 ± 60.75 min, cardiac arrest time of 51.33 ± 3.06 min, and a hospital stay of 13.67 ± 5.51 days. The most common complications were surgical-site infection and shock (2/3). Only one patient died (1/3).
Conclusion
Evidence involving the accurate diagnosis, prevention, and management of type-1 non-dissecting ruptured Kommerell's aneurysms is scarce. Additional, robust, and more extensive studies are required. The selection of the appropriate surgical approach is challenging. In our experience, Frozen Elephant Trunk was feasible for patients requiring emergent surgical repair. However, other hybrid procedures can be performed.
Transplantation and non-invasive longitudinal in vivo imaging of parathyroid cells
Authors: Bränström Robert , Krieken Pim van , Fröbom Robin , Juhlin Carl Christofer , Dicker Andrea , Leibiger Barbara , Leibiger Ingo B. , Berggren Per-Olof , Aspinwall Craig A. 
Published: October 2022

Abstract
The parathyroid cell is a critical physiological regulator of extracellular Ca2+ levels via secretion of parathyroid hormone (PTH). Regulation of PTH secretion is complex, not fully understood and represents a unique feature between extracellular Ca2+, intracellular Ca2+, and hormone secretion. One major problem in parathyroid research is the difficulty maintaining surgically procured cells ex vivo to allow detailed cellular characterization. Here, we introduce a new platform for transplantation and non-invasive in vivo imaging of parathyroid cells using the anterior chamber of the eye as a transplantation site. Parathyroid tissue pieces transplanted into the mouse eye engrafted on the iris became vascularized and retained cellular composition. Transplanted animals showed elevated PTH levels as a sign of a functional graft. In vivo confocal microscopy allowed repetitive monitoring of parathyroid graft morphology and vascularization. In summary, there is an urgent need for novel methods to characterize complex cellular processes in parathyroid cells. We show a novel method and strategy for non-invasive in vivo investigations that can be applied under physiological and pathological conditions, enabling deepening knowledge of parathyroid physiology and pathology.
Graph-DTI: A new Model for Drug-target Interaction Prediction Based on Heterogenous Network Graph Embedding
Authors: Qu Xiaohan , Du Guoxia , Hu Jing , Cai Yongming 
Published: October 2022

Abstract

Background
Accurate prediction of drug-target interactions (DTIs) can guide the drug discovery process and thus facilitate drug development. Most existing computational models for machine learning tend to focus on integrating multiple data sources and combining them with popular embedding methods. However, researchers have paid less attention to the correlation between drugs and target proteins. In addition, recent studies have employed heterogeneous network graphs for DTI prediction, but there are limitations in obtaining rich neighborhood information among nodes in heterogeneous network graphs.
Results
Inspired by recent years of graph embedding and knowledge representation learning, we develop a new end-to-end learning model, called Graph-DTI, which integrates various information from heterogeneous network data and automatically learns topology-preserving representations of drugs and targets to facilitate DTI prediction. Our framework consists of three main building blocks. First, we integrate multiple data sources of drugs and target proteins and build a heterogeneous network from a collection of datasets. Second, the heterogeneous network is formed by extracting higher-order structural information using a GCN-inspired graph autoencoder to learn the nodes (drugs, proteins) and their topological neighborhood representations. The last part is to predict the potential DTIs and then send the trained samples to the classifier for binary classification.
Conclusions
The substantial improvement in prediction performance compared to other baseline DTI prediction methods demonstrates the superior predictive power of Graph-DTI. Moreover, the proposed framework has been successful in ranking drugs corresponding to different targets and vice versa. All these results suggest that Graph-DTI can provide a powerful tool for drug research, development and repositioning.
The labour market dilemma of young urban women in India:An outcome of family welfare optimization
Authors: Parida Jajati Keshari , Bhagavatula Niharika 
Published: October 2022

Abstract
This paper explains the patterns and determinants of labour market participation behaviour of young urban women in India using a theoretical model. Based on the National Sample Survey (NSS) data, and through probit regression results, it argues that the labour market participation decision of young women is an outcome of the joint utility maximization behaviour of their family. The standard of living of the family, market wage, other family characteristics including number of children, adult women in the family, elderly members, and occupation, and gender of the head etc., are significantly determining their labour market participation; apart from women’s individual characteristics like age (experience) and level of education and training. The empirical result also reflects that the “discouraged worker effect” is stronger than “added worker phenomenon”. In this context, measure to create jobs in modern services could help to boost the stagnant female labour force participation upward in urban India.
Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms
Authors: Teughels Wim , Holm Wannes Van , Carvalho Rita , Delanghe Lize , Eilers Tom , Zayed Naiera , Mermans Fabian , Bernaerts Kristel , Boon Nico , Claes Ingmar , Lebeer Sarah 
Published: October 2022

Abstract
Several oral diseases are characterized by a shift within the oral microbiome towards a pathogenic, dysbiotic composition. Broad spectrum antimicrobials are often part of their treatment. However, with the rising antibiotic resistance, alternatives are increasingly desirable. Alternatively, supplying beneficial species through probiotics is increasingly showing beneficial results, however, these probiotics are rarely evaluated comparatively. In this study, the in vitro effects of three known- and three novel lactobacillus strains, together with four novel Streptococcus salivarius strains were comparatively evaluated for antagonistic effects on proximal agar growth, antimicrobial properties of probiotic supernatant and the probiotic's effects on in vitro periodontal biofilms. Strain specific effects were observed as differences between genera and differences in efficacy within genera. While some of the lactobacillus candidates were able to reduce the periodontal pathobiont A. actinomycetemcomitans, the S. salivarius strains were not. However, the S. salivarius strains were more effective against P. intermedia, P. gingivalis and F. nucleatum. Most of the lactobacillus strains also negatively affected the prevalence commensal species within the biofilms, while this was lower for S. salivarius strains. Both within lactobacilli and streptococci some strains showed significantly more inhibition of the pathobionts, indicating the importance of proper strain selection. Additionally, some species showed reductions in non-target species, which can result in unexpected and unexplored effects on the whole microbiome.
A strain of Vibrio alginolyticus isolated from Azumapecten farreri and its pathogenic mechanism using CRISPRI-Cas9 technology
Authors: Ma jingxue , Zhang peiyu , Zheng minggang , wang bo , Gao ping , Qu lingyun , Zheng fengrong 
Published: October 2022

Abstract
Scallops have become an important aquaculture species in China because they contain high-quality protein, and scallops are important health food that combines multiple effects and high economic benefits. However, scallop aquaculture is perennially threatened by various pathogenic Vibrio species, leading to great economic losses. We obtained a strain of pathogenic bacteria, identified as V. alginolyticus, from the diseased Azumapecten farreri in the scallop farming area of Huangdao District in 2018, and V. alginolyticus is one of the major shellfish pathogens. We showed that V. alginolyticus was isolated and identified as a pathogen in Azumapecten farreri for the first time. In this study, we evaluated its morphology and performed a phylogenetic analysis based on 16S rRNA gene sequencing. In addition, we performed a preliminary analysis of its pathogenic mechanisms. The Hfq protein in V. alginolyticus is an important RNA-binding protein in the sensing (QS) system that not only affects the sensitivity of Vibrio to environmental stress but also regulates a variety of functions, such as cell membrane formation, motility, and virulence towards the host. However, its effect on the pathogenesis of V. alginolyticus to Azumapecten farreri is unclear. To further investigate the pathogenic mechanism of the Hfq protein in V. alginolyticus to Azumapecten farreri , we used the CRISPR-Cas9 system to target and deplete the Hfq gene fragment in V. alginolyticus and obtained the mutant strain V. ΔHfq - . We found that the peripheral flagellum of the mutant strain was lost, which reduced the motility of V. alginolyticus . Therefore, deletion of target genes by the CRISPR/Cas9 genome editing system confirmed that the Hfq protein played a key role in reducing the ability of V. alginolyticus to infect Azumapecten farreri . In conclusion, our current findings provided valuable insights into the healthy culture of scallops.
Comparative analysis of two kinds of garlic seedings: qualities and transcriptional landscape
Authors: Ai Penghui , Xue Jundong , Zhu Yifei , Tan Wenchao , Wu Yifei , Wang Ying , Li Zhongai , Shi Zhongya , Kang Dongru , Zhang Haoyi , Jiang Liwen , Wang Zicheng 
Published: October 2022

Abstract
Background: Facility cultivation is widely applied to meet the increasing demand for high yield and quality, with light intensity and light quality being major limiting factors. However, how changes in the light environment affect development and quality are unclear in garlic. When garlic seedlings are grown, they can also be exposed to blanching culture conditions of darkness or low-light intensity to ameliorate their appearance and modify their bioactive compounds and flavor.
Results: In this study, we determined the quality and transcriptomes of 14-day-old garlic and blanched garlic seedlings (green seedlings and blanched seedlings) to explore the mechanisms by which seedlings integrate light signals. Seedling quality was significantly lower in blanched garlic seedlings. We also identified 5,783 differentially expressed genes between green and blanched garlic seedlings. Gene Ontology term enrichment analysis revealed an overrepresentation of genes mainly involved in secondary metabolism, catalytic activity, and binding. Similarly, the Kyoto Encyclopedia of Genes and Genomes analysis suggested enrichment for plant-pathogen interactions, phytohormone signaling, mitogen-activated protein kinase signaling, and other metabolic processes. In agreement, we identified multiple types of transcription factor genes involved in plant-pathogen interactions, plant phytohormone signaling, and biosynthesis of secondary metabolites among the differentially expressed genes between green and blanched garlic seedlings.
Conclusions: Blanching culture is one facility cultivation mode that promotes chlorophyll degradation, thus changing the outward appearance of crops, and improves their flavor. A transcriptome analysis of garlic seedlings grown in light and darkness conditions. The large number of DEGs identified confirmed the difference of the regulatory machinery under two culture system. This study increases our understanding of the regulatory network integrating light and darkness signals in garlic seedlings and provides a useful resource for the genetic manipulation and cultivation of blanched garlic seedlings.
Research on the ring formation mechanism of magnesian flux pellets in rotary kiln
Authors: Guo Zongheng , Tian Tielei , Zhang Yuzhu 
Published: October 2022

Abstract
The forming of rings was a key problem of magnesian flux pellets in rotary kiln, which seriously limited production efficiency. Pellet powder and flux were the raw materials of the ring. Based on this, the bonding strength, melting behavior and microstructure of pellet powder and its mixed powder with flux were investigated. The influence of basicity on ring behavior of pellet powder was analyzed, and ring formation mechanism of magnesian flux pellets was clarified. The results showed that acid pellet powder was not easy to form rings due to lower bonding strength of briquettes. Due to changes in bonding process after mixing of flux, magnesian flux pellet powder produced ferrite and silicate liquid phase with lower melting point, which promoted diffusion and recrystallization of hematite and enhanced compressive strength of briquettes, then finally caused rings to form. Moreover, it is required to control roasting temperature below 1200℃, which is a necessary condition for magnesian flux pellet powder to form an initial ring which was easy to be destroyed.
Alteration analysis of refractories bricks for industrial horizontal anodes baking furnace
Authors: Tankeu Jeanne Solange Ntchayi , Gnamsi Guy Molay Tchapga , Tchamba Arlin Bruno , Ninla Patrick Lemougna , Kamseu Elie , Guidana Marcel , Nkeng George Elambo , Kausik Dana , Njopwouo Daniel 
Published: October 2022

Abstract
This study highlights the alteration of refractory bricks for anode baking furnace for unused and used bricks after 96 baking cycles. The chemical composition of the unused bricks included silica (51.37wt.%) and alumina (34.05wt.%). A decrease of compressive strength from 48.45 MPa to 32 MPa respectively for unused and used bricks were observed. The bulk density obtained by mercury intrusion porosimeter method decreased from 2.48 to 2.38 g/cm3for unused and used bricks; while the bulk density by archimades method increased from 2.29 to 2.49 g/cm3. The water absorption by Archimedes method is decreased from 6.65wt.% to 5.05wt.%. While the total porosity obtained by mercury intrusion porosimeter increased from 15.41wt.% to 17.21wt.% for unused and used bricks. In the same way the total cumulative pore increased from 62.05 mm3/g to 72.35 mm3/g for unused and used brick. While the average pore radius decreased from 5.65 µm to 1.06 µm respectively for unused and used brick due to the deposition of C and particles inside the pores. The alteration of the hot side of brick is due by the decrease of Si and Ti with increase of Fe; while the alteration of anode side is due to the increase of Si and decrease of Al. The anode side of brick is deterioted rapidly than hot side of brick. Mullite peak seems to be not change, suggesting that a very compacted brick with high mullite content and high alumina content 75-90wt.% seems to be the best matrix for bricks in anode baking furnace.