bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation
Authors: Li Yangping , Wang Feng , Teng Peng , Ku Li , Chen Li , Feng Yue , Yao Bing 
Published: February 2022

AbstractBackgroundCircular RNAs (circRNAs), a novel class of poorly conserved non-coding RNAs that regulate gene expression, are highly enriched in the human brain. Despite increasing discoveries of circRNA function in human neurons, the circRNA landscape and function in developing human oligodendroglia, the myelinating cells that govern neuronal conductance, remains unexplored. Meanwhile, improved experimental and computational tools for the accurate identification of circRNAs are needed.ResultsWe adopt a published experimental approach for circRNA enrichment and develop CARP (CircRNA identification using A-tailing RNase R approach and Pseudo-reference alignment), a comprehensive 21-module computational framework for accurate circRNA identification and quantification. Using CARP, we identify developmentally programmed human oligodendroglia circRNA landscapes in the HOG oligodendroglioma cell line, distinct from neuronal circRNA landscapes. Numerous circRNAs display oligodendroglia-specific regulation upon differentiation, among which a subclass is regulated independently from their parental mRNAs. We find that circRNA flanking introns often containcis-regulatory elements for RNA editing and are predicted to bind differentiation-regulated splicing factors. In addition, we discover novel oligodendroglia-specific circRNAs that are predicted to sponge microRNAs, which co-operatively promote oligodendroglia development. Furthermore, we identify circRNA clusters derived from differentiation-regulated alternative circularization events within the same gene, each containing a common circular exon, achieving additive sponging effects that promote human oligodendroglia differentiation.ConclusionsOur results reveal dynamic regulation of human oligodendroglia circRNA landscapes during early differentiation and suggest critical roles of the circRNA-miRNA-mRNA axis in advancing human oligodendroglia development.
Efficacy of cognitive behavioral therapy on aggressive behavior in children with attention deficit hyperactivity disorder and emotion dysregulation: study protocol of a randomized controlled trial
Authors: Vacher C. , Romo L. , Dereure M. , Soler M. , Picot M. C. , Purper-Ouakil D. 
Published: February 2022

AbstractBackgroundAttention deficit hyperactivity disorder (ADHD) is frequently associated with emotional dysregulation (ED). ED is characterized by excessive and inappropriate emotional reactions compared to social norms, uncontrolled and rapid shifts in emotion, and attention focused on emotional stimuli. Few studies have evaluated non-pharmacological interventions to improve ED in children with ADHD. The current randomized controlled trial assesses the efficacy of a cognitive behavioral therapy (CBT) intervention compared with a theater-based intervention (TBI) in children with ADHD and ED.MethodsSixty-eight 7- to 13-year-old children with ADHD and ED will be recruited and randomly assigned to the CBT or TBI group. CBT aims to reduce ED by teaching anger management strategies. TBI seeks to reduce ED by improving emotion understanding and expression through mimics and movement. In both groups, children participate in 15 1-h sessions, and parents participate in 8 sessions of a parent management program. The primary outcome measure is the change in the “Aggression” sub-score of the Child Behavior Checklist (CBCL). Secondary outcome measures include overall impairment (Children’s Global Assessment Scale, Strengths and Difficulties Questionnaire), personality profile (Hierarchical Personality Inventory for Children), executive function (Behavioral Rating Inventory of Executive Function), quality of life (Kidscreen-27), parental stress (Parenting Stress Index, 4th edition), parental depression (Beck Depression Inventory-II), and impact of child disorders on the quality of the family life (Parental Quality of Life and Developmental Disorder).DiscussionChildren with ADHD and ED are at risk of functional impairment and poor outcomes and have specific therapeutic needs. This randomized controlled trial wants to assess non-pharmacological treatment options for this population.Trial registrationClinicaltrials.gov.NCT03176108. Registered on June 5, 2017.
Bottom-up synthetic ecology study of microbial consortia to enhance lignocellulose bioconversion
Authors: Lin Lu 
Published: February 2022

AbstractLignocellulose is the most abundant organic carbon polymer on the earth. Its decomposition and conversion greatly impact the global carbon cycle. Furthermore, it provides feedstock for sustainable fuel and other value-added products. However, it continues to be underutilized, due to its highly recalcitrant and heterogeneric structure. Microorganisms, which have evolved versatile pathways to convert lignocellulose, undoubtedly are at the heart of lignocellulose conversion. Numerous studies that have reported successful metabolic engineering of individual strains to improve biological lignin valorization. Meanwhile, the bottleneck of single strain modification is becoming increasingly urgent in the conversion of complex substrates. Alternatively, increased attention has been paid to microbial consortia, as they show advantages over pure cultures, e.g., high efficiency and robustness. Here, we first review recent developments in microbial communities for lignocellulose bioconversion. Furthermore, the emerging area of synthetic ecology, which is an integration of synthetic biology, ecology, and computational biology, provides an opportunity for the bottom-up construction of microbial consortia. Then, we review different modes of microbial interaction and their molecular mechanisms, and discuss considerations of how to employ these interactions to construct synthetic consortia via synthetic ecology, as well as highlight emerging trends in engineering microbial communities for lignocellulose bioconversion.
Genetic and molecular biology of gastric cancer among Iranian patients: an update
Authors: Abbaszadegan Mohammad Reza , Mojarrad Majid , Rahimi Hamid Reza , Moghbeli Meysam 
Published: February 2022

AbstractBackgroundThere is a declining trend of gastric cancer (GC) incidence in the world during recent years that is related to the development of novel diagnostic methods. However, there is still a high ratio of GC mortality among the Iranian population that can be associated with late diagnosis. Despite various reports about the novel diagnostic markers, there is not any general and standard diagnostic panel marker for Iranian GC patients. Therefore, it is required to determine an efficient and general panel of molecular markers for early detection.Main body of the abstractIn the present review, we summarized all of the reported markers until now among Iranian GC patients to pave the way for the determination of a population-based diagnostic panel of markers. In this regard, we categorized these markers in different groups based on their involved processes to know which molecular process is more frequent during the GC progression among Iranians.ConclusionWe observed that the non-coding RNAs are the main factors involved in GC tumorigenesis in this population.
Osteoblasts pattern endothelium and somatosensory axons during zebrafish caudal fin organogenesis
Authors: Bump Rosalind G. , Goo Camille E. A. , Horton Emma C. , Rasmussen Jeffrey P. 
Published: February 2022

ABSTRACT
Skeletal elements frequently associate with vasculature and somatosensory nerves, which regulate bone development and homeostasis. However, the deep, internal location of bones in many vertebrates has limited in vivo exploration of the neurovascular-bone relationship. Here, we use the zebrafish caudal fin, an optically accessible organ formed of repeating bony ray skeletal units, to determine the cellular relationship between nerves, bones and endothelium. In adult zebrafish, we establish the presence of somatosensory axons running through the inside of the bony fin rays, juxtaposed with osteoblasts on the inner hemiray surface. During development we show that the caudal fin progresses through sequential stages of endothelial plexus formation, bony ray addition, ray innervation and endothelial remodeling. Surprisingly, the initial stages of fin morphogenesis proceed normally in animals lacking either fin endothelium or somatosensory nerves. Instead, we find that sp7+ osteoblasts are required for endothelial remodeling and somatosensory axon innervation in the developing fin. Overall, this study demonstrates that the proximal neurovascular-bone relationship in the adult caudal fin is established during fin organogenesis and suggests that ray-associated osteoblasts pattern axons and endothelium.
Endoplasmic reticulum–mitochondria signaling in neurons and neurodegenerative diseases
Authors: Markovinovic Andrea , Greig Jenny , Martín-Guerrero Sandra María , Salam Shaakir , Paillusson Sebastien 
Published: February 2022

ABSTRACT
Recent advances have revealed common pathological changes in neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis with related frontotemporal dementia (ALS/FTD). Many of these changes can be linked to alterations in endoplasmic reticulum (ER)–mitochondria signaling, including dysregulation of Ca2+ signaling, autophagy, lipid metabolism, ATP production, axonal transport, ER stress responses and synaptic dysfunction. ER–mitochondria signaling involves specialized regions of ER, called mitochondria-associated membranes (MAMs). Owing to their role in neurodegenerative processes, MAMs have gained attention as they appear to be associated with all the major neurodegenerative diseases. Furthermore, their specific role within neuronal maintenance is being revealed as mutant genes linked to major neurodegenerative diseases have been associated with damage to these specialized contacts. Several studies have now demonstrated that these specialized contacts regulate neuronal health and synaptic transmission, and that MAMs are damaged in patients with neurodegenerative diseases. This Review will focus on the role of MAMs and ER–mitochondria signaling within neurons and how damage of the ER–mitochondria axis leads to a disruption of vital processes causing eventual neurodegeneration.
Preparation and Properties of Composite Phase Change Material Based on Bentonite and Novel Eutectic Mixture of Organic Carbonates
Authors: Nguyen Tung N , Trinh Hung T , Le Giang H , Bui Minh Q , Nguyen Hai D 
Published: February 2022

Abstract
Composite phase change materials were prepared from bentonite and a novel eutectic mixture of organic carbonates. The bentonite was firstly modified by ion exchange reaction with cetrimonium chloride, then used to adsorb the eutectic mixture by solution intercalation method. Initial investigation showed that at the maximum adsorption ratio of 60%, the prepared material showed good shape stability while retaining suitable melting temperature and good latent heat capability of 30.21 °C and 84.03 J g−1, respectively.
Clarifying Optimal Sodium InTake In Cardiovasular and Kidney (COSTICK) Diseases: a study protocol for two randomised controlled trials
Authors: Smyth Andrew , Yusuf Salim , Kerins Claire , Corcoran Colette , Dineen Roisin , Alvarez-Iglesias Alberto , Ferguson John , McDermott Suzanne , Hernon Orlaith , Ranjan Ritika , Nolan Aoife , Griffin Matthew , O'Shea Paula , Canavan Michelle , O'Donnell Martin 
Published: February 2022

Background: While low sodium intake (<2.3g/day) is recommended for all, there is uncertainty about feasibility and net cardiovascular effects. In COSTICK, we evaluated the effects of a dietary counselling intervention (reduced sodium intake) on intermediate cardiorenal outcomes in patients with (STICK) and without (COSIP) mild/moderate kidney disease. Methods: This is a protocol for two phase IIb randomised, two-group, parallel, open-label, controlled, single centre trials. Participants were aged >40 years with stable blood pressure, unchanged anti-hypertensive medications, willing to modify diet and provided written informed consent. Participants were excluded for abnormal sodium handling, heart failure, high dose diuretics, immunosuppression, pregnancy/lactation, postural hypotension, cognitive impairment, high or low body mass index (BMI) or inclusion in another trial. STICK participants had estimated glomerular filtration rate (eGFR) 30-60ml/min/1.73m2 and were excluded for acute kidney Injury, rapidly declining eGFR; known glomerular disease or current use of non-steroidal anti-inflammatory drugs. For COSIP, participants were excluded for known kidney or cardiovascular disease. Participants were randomized to usual care only (healthy eating) or an additional sodium lowering intervention (target <100mmol/day) through specific counseling (sodium use in foods, fresh over processed foods, sodium content of foods and eating outside of home). In STICK the primary outcome is change in 24-hour urinary creatinine clearance. In COSIP, the primary outcome is change in five biomarkers (renin, aldosterone, high sensitivity troponin T, pro-B-type natriuretic peptide and C-reactive protein). Our primary report (COSTICK), reports six biomarker outcome measures in the entire population at 2 years follow-up. Discussion: These Phase II trials will explore uncertainty about low sodium intake and cardiovascular and kidney biomarkers, and help determine the feasibility of low sodium intake. Trial results will also provide preliminary information to guide a future definitive clinical trial, if indicated. Trial registration: STICK: ClinicalTrials.gov NCT02738736 (04/04/2016); COSIP: ClinicalTrials.gov NCT02458248 (15/05/2016)
VSE-TAKI AS A MARKER OF MONOLOGICAL DISCOURSE CONTRADICTION
Authors: Tashlykova Marina B. , Loshanina Maria N. 
Published: February 2022

Background. The research was carried out within the project «Oral history of Irkutsk and technologies oral history in an interdisciplinary perspective». An analysis of the array of in-depth interviews obtained during the implementation of the project reveals a significant number of fragments reflecting the internal conflict of the speaker’s ideas about some fragment of the real world, which is in the center of his attention at a certain moment of speech. A current issue is to identify linguistic and discourse markers that objectify the presence of this conflict. One of these markers is vse-taki to which the article is devoted.
The purpose of the work is to clarify the idea of the status of this unit, its semantic, pragmatic and functional features, based on the understanding of the existing lexicographic tradition and the analysis of empirical material.
The material of the research is the data of traditional explanatory dictionaries and the Active dictionary of the Russian language, as well as examples of contextual use of vse-taki, extracted from the RNC. Methods and techniques of definitional, structural-semantic, contextual and corpus analysis are used.
The results of the research showed that vse-taki should be qualified as a particle which is used (a) as a marker of the opposition of the existing state of affairs to a hypothetical, but more standard one; (b) as a marker of the reason for the implementation of one of the two alternatives; (c) as a marker of the speaker’s emotional reaction to the coincidence of fragments of the mental and real world.
The field of practical implications of the results is theoretical semantics and discourse analysis (including in social research). The results can be used in semantics, pragmatics, discourse theory university courses.
COMPARATIVE ANALYSIS OF ORIGIN AND EVOLUTION OF SIGN LANGUAGES
Authors: Galieva Dina A. , Naurazbaeva Liliya V. 
Published: February 2022

The object of the research is to analyze sign languages which have been an essential aspect of communication throughout human history of the disabled. It aims to examine the main stages of development of sign languages.
The subject of the research is sign languages of the deaf and the hearing impaired.
The research methods include a theoretical analysis of the works of Russian and foreign linguists and the method of comparative analysis.
The results of the analysis of the main stages of sign languages let us come to the conclusion that the status of sign languages which have passed the historical gradual development responding to the needs of a developing society. The following aspects such as the verbal national language, linguistic schools, the culture of the people have a huge impact on the formation and development of any sign language. It should be noted that sign languages as well as verbal languages are related to each other. They are functionally complete (natural) languages with their own, sometimes rather complex internal mechanism.
Crise climática e reconfiguração do romance contemporâneo em The Ministry for the Future, de Kim Stanley Robinson
Authors: Gomes Anderson Soares 
Published: February 2022

Este artigo tem como objetivo investigar as maneiras pelas quais o romance The Ministry for the Future (2020), de Kim Stanley Robinson, expande possibilidades da ficção contemporânea diante da crise climática. O texto será organizado a partir de dois focos de análise: a validade do gênero “ficção literária” para narrar o Antropoceno; e o questionamento da distinção entre os conceitos de natureza e cultura face à catástrofe ambiental. Sendo assim, este artigo pretende mostrar que The Ministry for the Future apresenta estratégias de reconfiguração do romance contemporâneo considerando o contexto da mudança climática. Palavras-chave: romance, crise climática, natureza, cultura, Kim Stanley Robinson
The primary stability of the femoral component in cemented single and twin peg Oxford unicompartmental knee arthroplasty under adverse conditions
Authors: Eckert Johannes Adrian , Bitsch Rudi G. , Sonntag Robert , Reiner Tobias , Schwarze Martin , Jaeger Sebastian 
Published: February 2022

Aims The cemented Oxford unicompartmental knee arthroplasty (OUKA) features two variants: single and twin peg OUKA. The purpose of this study was to assess the stability of both variants in a worst-case scenario of bone defects and suboptimal cementation. Methods Single and twin pegs were implanted randomly allocated in 12 pairs of human fresh-frozen femora. We generated 5° bone defects at the posterior condyle. Relative movement was simulated using a servohydraulic pulser, and analyzed at 70°/115° knee flexion. Relative movement was surveyed at seven points of measurement on implant and bone, using an optic system. Results At the main fixation zone, the twin peg shows less relative movement at 70°/115°. At the transition zone, relative movements are smaller for the single peg for both angles. The single peg shows higher compression at 70° flexion, whereas the twin peg design shows higher compression at 115°. X-displacement is significantly higher for the single peg at 115°. Conclusion Bony defects should be avoided in OUKA. The twin peg shows high resilience against push-out force and should be preferred over the single peg. Cite this article: Bone Joint Res 2022;11(2):82–90.
Karyotype analysis from four species of edible plants in northeastern Thailand
Authors: Saensouk Surapon , Saensouk Piyaporn , Saen-in Natkamon 
Published: February 2022

Karyotype analysis from four species of edible plants, namely one species from family Cannaceae (Canna indicaL.), one species including 2 variants from family Araceae (Colocasia esculenta(L.) Schott, green taro and black taro) and two species from family Apiaceae (Eryngium foetidum L. and Centella asiatica (L.) Urb.) in Maha Sarakham province, northeastern Thailand were determined from root tips. The results showed that the chromosome numbers and karyotype formulae were 2n= 28 = 14m + 10sm + 4st (2sat) with NF = 56 for green taro, 2n= 42 = 20m
+ 20sm + 2st (3sat) with NF = 84 for black taro, 2n = 18 = 18sm with NF = 36 for Centella asiatica (L.) Urb., 2n = 16 = 2m +14 sm with NF = 32 for Eryngium foetidum L., 2n= 27 = 2m
+ 25sm with NF = 54 for Canna indica L. Both taros had differences in the chromosome numbers and karyotype formulae. The chromosomes of both taros were the asymmetrical karyotype. While, the chromosome structure of both taros, including the number of metacentric, submetacentric and subtelocentric as well as the position of satellite-chromosomes, were found to be different, which indicated inter-and intra-specific variations in this species. Therefore, the chromosome number, karyotype formula, chromosome structure, position of satellite-chromosomes and idiogram can be used for classification of both taros in this study. Moreover, idiograms of both taros were reported for the first time. The karyotype, NF, RL, CI and
ideogram of Centella asiatica(L.) Urb., Eryngium foetidum L. and Canna indica L. were reported for the first time.
Comparing ethnobotanical knowledge of medicinal plants between community health workers and local experts in the “Mata da Paraíba” zone, northeastern Brazil
Authors: PAIVA MAIA ANNE CAROLINE , DA COSTA FERREIRA EZEQUIEL , MARQUES DE LUCENA CAMILLA , DOS SANTOS SOUZA ANDRÉ , DIAS DA CRUZ DENISE , FARIAS PAIVA DE LUCENA REINALDO 
Published: February 2022

Abstract. Paiva Maia AC, da Costa Ferreira E, Marques de Lucena C, dos Santos Souza A, Dias da Cruz D, Paiva de Lucena RF. 2021. Comparing ethnobotanical knowledge of medicinal plants between community health workers and local experts in the “Mata da Paraíba” zone, northeastern Brazil. Biodiversitas 22: 5606-5616. The use of medicinal plants is an old practice in a society that has been transmitted to this day, with local experts recognized in many communities and being encouraged through public policies. In Brazil, Community Health Agents (CHWs) can be important for the dissemination of this practice, due to their work with the Health System and the community. This study aimed to compare the knowledge of medicinal plants between CHWs and local experts in the community of Timbó, the municipality of Jacaraú (Paraíba, northeastern Brazil). Semi-structured interviews were conducted addressing local names of plants, their indications, parts used, and preparation methods. The data on the species local importance were analyzed through the use value (UV) and relative importance (RI) methods. Local experts cited more species than CHWs. There was great local importance of some species both among local specialists and among CHWs. However, some species were highlighted exclusively among local specialists. Decoction and infusion stood out among the preparation methods and leaf, flower, and seed were the most prominent parts used. The diseases treated with plants are mainly related to the respiratory and digestive systems. Our findings show a correspondence between the CHWs’ knowledge and local experts’ knowledge, which leads us to believe that experts and CHWs may be sharing the same local knowledge in the study community.
The Effect of Kerf Thickness on Hardwood Log Recovery
Authors: Thomas R. Edward , Buehlmann Urs 
Published: February 2022

Abstract
When sawing a log into lumber or other products, the saw blade removes material to separate the wood fibers between the resulting two parts, a loss of material that is commonly referred to as saw kerf. Thicker kerfs result in greater waste and less material available to produce lumber. Over the past decades, with the advancement of materials and technology, saw blade thickness has decreased. However, the reduction in material loss owing to a reduction in saw kerf may not always translate into a statistically significant increase in lumber product recovery. In this study, we explored the effect of saw kerf thickness on lumber recovery for a range of hardwood log diameters using the US Forest Service's Log Recovery Analysis Tool (LORCAT) sawmill simulation tool. Results indicate that the recovery gains realized depend upon the log diameters sawn, the lumber target thickness, and the change (reduction) in the thickness of the saw kerf.