bluuebunny/crossref_metadata_2025_split huggingface dataset:
LPS stimulation stabilizes HIF‐1α by enhancing HIF‐1α acetylation via the PARP1‐SIRT1 and ACLY‐Tip60 pathways in macrophages
Authors: Chen Qiang , Cui Kun , Zhao Zengqi , Xu Xiang , Liu Yongtao , Shen Yanan , Chen Fan , Mai Kangsen , Ai Qinghui 
Published: June 2022

AbstractHypoxia and inflammatory mediators stabilize hypoxia‐inducible factor (HIF)‐1α through posttranslational modifications, such as phosphorylation and succinylation. Here, we identified sirtuin 1 (SIRT1) and 60 kDa Tat‐interactive protein (Tip60)‐mediated acetylation as another critical posttranslational modification that regulates HIF‐1α protein stability under lipopolysaccharide (LPS) stimulation. Mechanistically, DNA damage induced by excessive reactive oxygen species (ROS) activated poly (ADP‐ribose) polymerase 1 (PARP1) to consume oxidized nicotinamide adenine dinucleotide (NAD+). Correspondingly, SIRT1 activity was decreased with the decline in NAD+ levels, resulting in increased HIF‐1α acetylation. LPS also activated the ATP‐citrate lyase (ACLY)‐Tip60 pathway to further enhance HIF‐1α acetylation. Acetylation contributed to HIF‐1α stability and exacerbated LPS‐induced inflammation. Thus, inhibiting HIF‐1α stability by decreasing its acetylation could partly alleviate LPS‐induced inflammation. In conclusion, we revealed the mechanism by which LPS stabilized HIF‐1α by increasing its acetylation via the PARP1‐SIRT1 and ACLY‐Tip60 pathways in fish macrophages. This study may provide novel insights for manipulation of HIF‐1α acetylation as a therapeutic strategy against inflammation from the perspective of acetylation in vertebrates.
Intradermal Botulinum Toxin A Injection Versus Topical 2% Glycopyrrolate for the Treatment of Primary Facial Hyperhidrosis: A Pilot Study and Review of Literature
Authors: Nofal Eman , Salem Somaia , Khashaba Shrook A. 
Published: June 2022

BACKGROUND
Facial hyperhidrosis (HH), a common problem with both cosmetic and psychological impact, interferes with quality of life. Wide range of treatment options is available for HH. Finding the most effective and yet a safe, tolerable option is the main target.

OBJECTIVE
To evaluate and compare clinical efficacy, safety, and tolerability of topical 2% glycopyrrolate versus intradermal Botulinum toxin A injection in facial HH treatment.

MATERIALS AND METHODS
Twenty-four patients with primary facial HH were randomly divided into 2 equal groups: Group A included patients treated by intradermal Botulinum toxin A injection and Group B included patients treated by topical glycopyrrolate gel 2%. Starch iodine test was performed before and after treatment to assess response, along with Hyperhidrosis Disease Severity Scale, Dermatology Life Quality Index (DLQI), and patient satisfaction.

RESULTS
Both modalities showed complete response in 75% of cases with a longer duration of action in botulinum toxin group up to 6 months. Side effects were minor and temporary. Both Hyperhidrosis Disease Severity Scale and DLQI showed statistically significant improvement after treatment.

CONCLUSION
Topical glycopyrrolate 2% showed comparable results to Botulinum toxin A in facial HH treatment with faster onset but shorter duration of action.
The effects of pain science education plus exercise on pain and function in chronic Achilles tendinopathy: a blinded, placebo-controlled, explanatory, randomized trial
Authors: Chimenti Ruth L. , Post Andrew A. , Rio Ebonie K. , Moseley G. Lorimer , Dao Megan , Mosby Hadley , Hall Mederic , de Cesar Netto Cesar , Wilken Jason M. , Danielson Jessica , Bayman Emine O. , Sluka Kathleen A. 
Published: June 2022

Abstract
Exercise is the standard of care for Achilles tendinopathy (AT), but 20% to 50% of patients continue to have pain following rehabilitation. The addition of pain science education (PSE) to an exercise program may enhance clinical outcomes, yet this has not been examined in patients with AT. Furthermore, little is known about how rehabilitation for AT alters the fear of movement and central nervous system nociceptive processing. Participants with chronic AT (N = 66) were randomized to receive education about AT either from a biopsychosocial (PSE) or from a biomedical (pathoanatomical education [PAE]) perspective. Simultaneously, all participants completed an exercise program over 8 weeks. Linear mixed models indicated that there were no differences between groups in (1) movement-evoked pain with both groups achieving a clinically meaningful reduction (mean change [95% CI], PSE: −3.0 [−3.8 to −2.2], PAE = −3.6 [−4.4 to −2.8]) and (2) self-reported function, with neither group achieving a clinically meaningful improvement (Patient-Reported Outcomes Measurement Information System Physical Function-PSE: 1.8 [0.3-3.4], PAE: 2.5 [0.8-4.2]). After rehabilitation, performance-based function improved (number of heel raises: 5.2 [1.6-8.8]), central nervous system nociceptive processing remained the same (conditioned pain modulation: −11.4% [0.2 to −17.3]), and fear of movement decreased (Tampa Scale of Kinesiophobia, TSK-17: −6.5 [−4.4 to −8.6]). Linear regression models indicated that baseline levels of pain and function along with improvements in self-efficacy and knowledge gain were associated with a greater improvement in pain and function, respectively. Thus, acquiring skills for symptom self-management and the process of learning may be more important than the specific educational approach for short-term clinical outcomes in patients with AT.
Delays in Door-to-Diuretic Time and 1-Year Mortality Among Patients With Heart Failure
Authors: Dzikowicz Dillon J. , Pokhrel Bhattarai Sunita , Ng Joyce , Zemanek Arthur L. , Carey Mary G. 
Published: June 2022

Background
Achieving prompt euvolemic state in heart failure (HF) is associated with reduced mortality. Time-sensitive metrics such as door-to-diuretic time, or the time between presentation and administration of intravenous diuretics, may be an important facilitator of achieving a faster euvolemic state and reducing mortality.

Objective
The aim of this study was to investigate whether reduced door-to-diuretic time was associated with lower odds of death among hospitalized patients with HF.

Methods
A retrospective chart review of patients with HF admitted to a medical center was performed between 2020 and 2021. Inclusion criteria were an International Classification of Diseases, 10th Revision code for HF with positive Framingham Criteria and the use of intravenous bolus furosemide. Exclusion criteria included ventricular assist devices, dialysis, and ultrafiltration therapy. Data collected from the medical records included demographics, echocardiography, staff notes, and medications. The end point was 1-year all-cause mortality. Descriptive statistics, t tests or median test, and multivariate logistic regression were used to describe the sample, evaluate group differences, and determine odds of mortality, respectively.

Results
Among 160 charts from patients with HF (age, 70 ± 14.4 years; 52%, n = 83, male; 53%, n = 85, ischemic cardiomyopathy; 83%, n = 134, New York Heart Association classes III–IV), 30% (n = 48) died within 1 year. The median diuretic dose was 40 mg (interquartile range, 20 mg), with a median time of administration of 247 minutes (4.12 hours) (interquartile range, 294 minutes to 4.9 hours). After covariate adjustment, prolonged door-to-diuretic time more than doubled (2.22; 95% confidence interval, 1.03–4.8; P = .04) the odds of 1-year mortality.

Conclusions
On the basis of this sample of charts from older highly symptomatic patients with HF, delayed door-to-diuretic time was associated with significantly greater odds of 1-year all-cause mortality.
Evaluation of the Efficacy of a Hypoglycemia Protocol to Treat Severe Hypoglycemia
Authors: Gilmore Lisa , Freeman Susan , Amarasekara Sathya , Maza Alfredo , Setji Tracy 
Published: June 2022

Purpose
The purpose of this quality improvement project was to evaluate the efficacy of the facility's Hypoglycemia Protocol when treating severe hypoglycemia (defined as blood glucose < 50 mg/dL).

Description
The diabetes clinical nurse specialists conducted a retrospective chart review of diabetic inpatients with severe hypoglycemia who were treated per the Hypoglycemia Protocol from October 1, 2017, through April 30, 2019. The primary outcome was achievement of euglycemia (defined as blood glucose ≥ 80 mg/dL) 15 to 30 minutes post treatment with either oral carbohydrates or intravenous dextrose.

Outcome
Two hundred twenty-two patients received treatment with oral carbohydrates versus 120 patients who received intravenous dextrose. Fifty patients receiving oral carbohydrates versus 106 patients receiving intravenous dextrose achieved euglycemia after 1 treatment. Compared with treatment with intravenous dextrose, the odds ratio of the rise in blood glucose to 80 mg/dL or greater within 15 to 30 minutes post treatment for a patient given oral carbohydrate was decreased by 97.2%.

Conclusion
Intravenous dextrose was more efficacious than oral carbohydrate treatment in patients with diabetes experiencing severe hypoglycemia. In response, the Hypoglycemia Protocol was revised to increase oral carbohydrate treatment for severe hypoglycemia and to expedite escalation from oral to intravenous treatment.
The Agile Clinical Nurse Specialist
Authors: Mamais Fotine , Jasdhaul Maninder , Gawlinski Anna , Lawanson-Nichols Mary , Kao Yuhan , Branom Raquel , Ansryan Lianna Z. 
Published: June 2022

Purpose/Objectives
This article describes the contributions of the clinical nurse specialist in navigating the challenges of the COVID-19 pandemic to ensure patient and staff safety while providing science-based quality of care.

Description
The group of clinical nurse specialists using advanced practice knowledge and skills within the 3 spheres of impact (ie, patient, organization, and nurse) developed and implemented strategies that supported frontline clinicians and met emerging organizational needs during the COVID-19 pandemic.

Outcomes
The clinical nurse specialist's agility was imperative in navigating the challenges of the pandemic to ensure the safety of patients and staff by providing strategies and standardization to workflow processes across the organization.

Conclusion
The group's combined clinical expertise and support of frontline nurses positioned the clinical nurse specialist to rapidly escalate the bedside nurse's concerns and provide recommendations to improve workflow while maintaining patient and staff safety.
Factors Associated With Patient Satisfaction Measured Using an Iterative Scale
Authors: Versluijs Yvonne , Fatehi Amirreza , Gonzalez Amanda I. , Reichel Lee , Laverty David , Ring David 
Published: June 2022

Background and Objectives:
Patient experience measures tend to have notable ceiling effects that make it difficult to learn from gradations of satisfaction to improve care. This study tested 2 different iterative satisfaction measures after a musculoskeletal specialty care visit in the hope that they might have less ceiling effect. We measured floor effects, ceilings effects, skewness, and kurtosis of both questionnaires. We also assessed patient factors independently associated with the questionnaires and the top 2 possible scores.

Methods:
In this cross-sectional study, 186 patients completed questionnaires while seeing 1 of 11 participating orthopedic surgeons in July and August 2019; the questionnaire measured: (1) demographics, (2) symptoms of depression, (3) catastrophic thinking in response to nociception, (4) heightened illness concerns, and (5) satisfaction with the visit on 2 iterative satisfaction scales. Bivariate and multivariable analyses sought associations of the explanatory variable with the satisfaction scales.

Results:
There is a small correlation between the 2 scales (r = 0.27; P < .001). Neither scale had a floor effect and both had a ceiling effect of 45%. There is a very small correlation between greater health anxiety and lower satisfaction measured with one of the scales (r = −0.16; P = .05).

Conclusion:
An iterative satisfaction questionnaire created some spread in patient experience data, but could not limit ceiling effects. Additional strategies are needed to remove ceiling effects from satisfaction measures.
False Positive Ventilation/Perfusion SPECT/CT Mismatch Mimicking Pulmonary Embolism in a Patient With Bilateral Lung Transplant
Authors: Sandach Patrick , Kleibrink Björn , Maier Sandra , Herrmann Ken , Hautzel Hubertus 
Published: June 2022

Abstract
Ventilation/perfusion SPECT/CT has very high sensitivity with little false-positive findings for diagnosing pulmonary embolism (PE). However, bronchopulmonary tumors or structural changes of the lungs' vasculature infrequently mimic PE. Here, a 59-year-old man presented with acute dyspnea and acute renal failure 5 years after bilateral lung transplant. Pulmonary ventilation/perfusion SPECT/CT was performed demonstrating a lobar mismatch of the left upper lung lobe indicative for PE. Bronchoscopy revealed local hyperemia of this lobe, indicating prolonged venous blood return. Subsequent CT angiography confirmed postsurgical upper pulmonary vein obliteration as final diagnosis. In conclusion, pulmonary vein obliteration might cause false-positive ventilation/perfusion SPECT/CT.
A Systematic Review on COVID-19 Vaccine preferences using Discrete Choice Experiments
Authors: Amani Adidja , Selenguai Helen Kamo , Puepi Yolande Djike , Dairou Iyale Astadjam , Kenmoe Sebastien , Nouko Ariane , Fabrice Zobel Cheuyem Lekeumo , Ngo Um Suzanne Sap , Koki Ndombo Paul Olivier , Mbacham Wilfried , Ongolo-Zogo Pierre 
Published: June 2022

AbstractObjectiveTo determine the attributes of COVID-19 vaccines that influence vaccine acceptance using a DCE through a systematic review.MethodsA systematic search was carried out for articles published up to November 2021 in the PubMed, Psycinfo, Embase, Web of Science, and Global Index Medicus databases. The electronic search algorithm consisted of the terms (Covid-19) AND (Vaccine) AND (discrete choice experiment).FindingsA total of 39 records were retrieved of which 18 duplicates were identified and removed. Of the remaining 21 records, 10 were excluded because they did not use a DCE approach. 11 studies were included in the meta-analyses with a total of 42 795 participants from three WHO regions. We examined 13 attributes of COVID-19 vaccine that influenced acceptance; cost, vaccine efficacy, number of doses, risk of side effects, proof of vaccination, vaccination setting, duration of immunity, doctor’s recommendation, proportion of acquaintances vaccinated, region of vaccine manufacture, background knowledge of herd immunity, life attenuated or mRNA, speed of vaccination development. The four attributes reported to influence COVID-19 vaccine preferences most worldwide were; high vaccine efficacy, low risk of side effects, long duration of immunity and low number of doses of the vaccine.ConclusionThe most preferred COVID-19 vaccine attributes should be taken into account by vaccine manufacturers and public health policy makers for better introduction and acceptance of COVID-19 vaccine to the world.
Energy Landscapes from Cryo-EM Snapshots: A Benchmarking Study
Authors: Dsouza Raison , Mashayekhi Ghoncheh , Etmaadpour Roshanak , Schwander Peter , Ourmazd Abbas 
Published: June 2022

AbstractBiomolecules undergo complex continuous conformational motions, a subset of which are functionally relevant. Understanding, and ultimately controlling biomolecular function are predicated on the ability to map continuous conformational motions and identify the functionally relevant conformational trajectories. For equilibrium and near-equilibrium processes, the function proceeds along minimum-energy pathways on one or more energy landscapes, because higher-energy conformations are only weakly occupied. With the growing interest in identifying functional trajectories on energy landscapes, the reliable mapping of energy landscapes has become paramount. In response, various data-analytical tools for determining structural variability are emerging. A key question concerns the veracity with which each data-analytical tool can extract functionally relevant conformational trajectories from a collection of singleparticle cryo-EM snapshots. Using synthetic data as an independently known ground truth, we benchmark the ability of four leading algorithms to determine biomolecular energy landscapes and identify the functionally relevant conformational paths on these landscapes. Such benchmarking is essential for systematic progress toward atomic-level movies of continuous biomolecular function.
Towards robust and generalizable super-resolution generative adversarial networks for magnetic resonance neuroimaging: a cross-population approach
Authors: Förster Leona Charlotte , da Costa Campos Lucas , Kocher Martin , Caspers Svenja 
Published: June 2022

AbstractMagnetic resonance imaging (MRI) is fundamental to neuroscience, where detailed structural brain scans improve clinical diagnoses and provide accurate neuroanatomical information. Apart from time-consuming scanning protocols, higher image resolution can be obtained with super resolution algorithms. We investigated the generalization abilities of Super Resolution Generative Adversarial Neural Networks (SRGANs) across different populations. T1-weighted scans from three large cohorts were used, spanning older subjects, newborns, and patients with brain tumor- or treatment-induced tissue changes. Upsampling quality was validated using synthetic and anatomical metrics. Models were first trained on each cohort, yielding high image quality and anatomical fidelity. When applied across cohorts, no artifacts were introduced by the SRGANs. SRGANs that were trained on a dataset combining all cohorts also did not induce any population-based artifacts. We showed that SRGANs provide a prime example of robust AI, where application on unseen populations did not introduce artifacts due to training data bias (e.g., insertion or removal of tumor-related signals and contrast inversion). This is an important step in the deployment of SRGANs in real-world settings.
Shaping Sounds with P300 Based Brain-Computer Musical Interface
Authors: Akkaya Uğur Can , Demirel Çağatay , de Lautour Reuben , İnce Gökhan 
Published: June 2022

AbstractThis paper describes the development and testing of a brain-computer musical interface (BCMI) that allows a user to select and transform one element of a musical texture by paying attention to that particular element. In order to realize the BCMI system mentioned, a comprehensive testing scheme was established which uses auditory evoked potentials to elicit P300 waves via averaging various types of stimuli. Resented sound stimuli were divided into multi-channel speaker setups to have better localization of user-focused sound stimuli. A sound synthesis model was developed for transforming its sound texture based on neural oscillations that were categorized with the help of a self-organizing map algorithm. In addition, an artificial neural network was used to predict the possible P300 waves that show the attentional focus of a subject. Most of the P300 waves were classified successfully for most of the participants. Promising results were achieved concerning the developed BCMI system. A neural network model was also utilized to predict the possible P300 waves, which show the subject’s selective attention. The majority of the participants were able to correctly classify P300 waves. The proposed BCMI system yielded promising results.
Lymphatic dysfunction in lupus contributes to cutaneous photosensitivity and lymph node B cell responses
Authors: Ambler William G. , Howlander Mir , Chalasani Madhavi Latha S. , Seltzer Ethan S. , Sim JiHyun , Shin Jinyeon , Schwartz Noa , Shipman William D , Dasoveanu Dragos , Carballo Camila B. , Sevim Ecem , Siddique Salma , Rodeo Scott , Erkan Doruk , Kataru Raghu P. , Mehrara Babak , Lu Theresa T. 
Published: June 2022

ABSTRACTPhotosensitivity in lupus and subsequent systemic disease flares contribute to disease burden and is incompletely understood. Skin communicates with lymphoid tissues via lymphatics and reduced lymphatic flow in murine models results in skin inflammation and autoimmunity. Here, we demonstrate that the skin of lupus patients and multiple murine lupus models are characterized by lymphatic flow dysfunction. Improving lymphatic flow in murine lupus models via manual lymphatic drainage or by utilizing a transgenic model with increased lymphatics ameliorated cutaneous photosensitivity and diminished draining lymph node germinal center B cell and plasmablast responses. Improved lymphatic flow limited B cell responses via acting on a stromal-immune circuit previously described by our lab. Increased lymphatic flow increases stromal CCL2, which modulates monocyte function and limits B cell responses. This work provides a link between cutaneous photosensitivity and systemic disease in lupus and suggests that improving lymphatic flow or targeting the lymph node microenvironment could be potential therapeutic targets.
Notch-1 mediates Epithelial-Mucosal Healing during Murine Colitis Recovery Phase
Authors: Luthra Latika , Patel Sahil R. , Bian Zhen , Liu Yuan , Merlin Didier , Garg Pallavi 
Published: June 2022

ABSTRACTInflammatory bowel disease (IBD) is marked by inflammation mediated epithelial-mucosal damage. The intestinal epithelial forms a tight barrier displaying two contrasting functions: restricting the entry of potentially harmful substances while, on the other hand allowing the selective passage of nutrients. The damaged epithelial-mucosal barrier causes the exposure of mucosa layers to luminal inflammatory contents. This eventually leads to the leaky epithelium, exposing the immune cells, release of various cytokines, and results in loss of epithelial homeostasis. Therefore, maintenance of healthy epithelial-mucosal lining is critical during IBD recovery. Notch signaling is an evolutionarily conserved molecular pathway crucial for the development and homeostasis of most tissues. Notably, the deregulation of Notch signaling is involved in IBD (Ulcerative colitis and Crohn’s disease). Notch signaling also plays a vital role in wound healing and tissue repair. Here, we investigated the role of Notch-1 signaling in wound healing and regeneration of colonic epithelium during colitis recovery phase by using conditional deletion of Notch-1 in colonic epithelium of mice. We used colonic carcinoma cell line HCT116 transiently transfected with Notch1 intracellular domain (NICD) to support in vivo data. We observed that deletion of Notch1 among mice was associated with compromised healing after colitis. Therefore, targeting the Notch-1 pathway might provide a novel therapeutic strategy for the patients recovering from colitis.
Evidence for grid-cell-related activity in the time domain
Authors: Peters-Founshtein Gregory , Dafni-Merom Amnon , Monsa Rotem , Arzy Shahar 
Published: June 2022

AbstractThe relation between the processing of space and time in the brain has been an enduring cross-disciplinary question. Grid cells have been recognized as a hallmark of the mammalian navigation system, with recent studies attesting to their involvement in organization of conceptual knowledge in humans. To determine whether grid-cell-like representations support temporal processing, we asked subjects to mentally simulate changes in age and time-of-day, each constituting “trajectory” in an age-day space, while undergoing fMRI. We found that grid-cell-like representations supported trajecting across this age-day space. Furthermore, brain regions concurrently coding past-to-future orientation positively modulated the magnitude of grid-cell-like representation in the left entorhinal cortex. Our findings suggest that temporal processing may be supported by spatially modulated systems, and that innate regularities of abstract domains may interface and alter grid-cell-like representations, similarly to spatial geometry.