bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of industrial (3000 L) production of Bacillus subtilis CW-S and its novel application for minituber and industrial-grade potato cultivation
Authors: Abuhena Md. , Al-Rashid Jubair , Azim Md. Faisal , Khan Md. Niuz Morshed , Kabir Md. Golam , Barman Nirmal Chandra , Rasul Noorain Munim , Akter Shahina , Huq Md. Amdadul 
Published: July 2022

AbstractA commercial plant probiotic product was developed employing Bacillus subtilis CW-S in submerged fermentation. The effects of molasses and urea on cell growth were investigated with the goal of low-cost manufacturing. Plackett–Burman and Central-Composite Design (CCD) were utilized to optimize production parameters to maximize productivity. The stability of the formulated product and its efficacy in cultivating minituber in aeroponics and industrial-grade potatoes in the field were assessed. The results showed that the medium BS10 (molasses and urea) produced satisfactory cell density (7.19 × 108 CFU/mL) as compared to the control (1.51 × 107 CFU/mL) and BS1-BS9 (expensive) media (1.84 × 107–1.37 × 109 CFU/mL). According to validated CCD results, optimized parameters fitted well in pilot (300 L; 2.05 × 109 CFU/mL) and industrial (3000 L; 2.01 × 109 CFU/mL) bioreactors, resulting in a two-fold increase in cell concentration over laboratory (9.84 × 108 CFU/mL) bioreactors. In aeroponics, CW-S produced excellent results, with a significant increase in the quantity and weight of minitubers and the survival rate of transplanted plantlets. In a field test, the yield of industrial-grade (> 55 mm) potatoes was increased with a reduction in fertilizer dose. Overall, the findings suggest that CW-S can be produced commercially utilizing the newly developed media and optimized conditions, making plant probiotics more cost-effective and accessible to farmers for crop cultivation, particularly in aeroponic minituber and industrial-grade potato production.
New insights on the evolutionary relationships between the major lineages of Amoebozoa
Authors: Tekle Yonas I. , Wang Fang , Wood Fiona C. , Anderson O. Roger , Smirnov Alexey 
Published: July 2022

AbstractThe supergroup Amoebozoa unites a wide diversity of amoeboid organisms and encompasses enigmatic lineages that have been recalcitrant to modern phylogenetics. Deep divergences, taxonomic placement of some key taxa and character evolution in the group largely remain poorly elucidated or controversial. We surveyed available Amoebozoa genomes and transcriptomes to mine conserved putative single copy genes, which were used to enrich gene sampling and generate the largest supermatrix in the group to date; encompassing 824 genes, including gene sequences not previously analyzed. We recovered a well-resolved and supported tree of Amoebozoa, revealing novel deep level relationships and resolving placement of enigmatic lineages congruent with morphological data. In our analysis the deepest branching group is Tubulinea. A recent proposed major clade Tevosa, uniting Evosea and Tubulinea, is not supported. Based on the new phylogenetic tree, paleoecological and paleontological data as well as data on the biology of presently living amoebozoans, we hypothesize that the evolution of Amoebozoa probably was driven by adaptive responses to a changing environment, where successful survival and predation resulted from a capacity to disrupt and graze on microbial mats-a dominant ecosystem of the mid-Proterozoic period of the Earth history.
Association between interleukin 10 (IL-10) polymorphisms and leishmaniasis progression: a systematic review and meta-analysis
Authors: da Silva Renata Rocha , Vasconcelos Fernanda de Santana Fontes , Tavares Débora dos Santos , dos Santos Priscila Lima 
Published: July 2022

AbstractInterleukin 10 (IL-10) is associated with the progression of leishmaniasis because it inhibits the leishmanicidal action of macrophages and the production of mediators such as IFN-γ and nitric oxide. Studies have shown that specific polymorphisms are associated with the regulatory role of IL-10 and the development of more relevant clinical forms of leishamaniasis. We performed a systematic review and meta-analysis to determine whether single nucleotide polymorphisms (SNPs) of IL-10 influence the progression of leishmaniasis. The selected articles were read in full and only those consistent with the eligibility criteria were included in our study. Seven studies were eligible according to the inclusion criteria and were included in the present systematic review, but only five were subjected to statistical analysis. The pooled odds ratios showed no significant association between the rs1800871 SNP and the progression of leishmaniasis in all genotype models, including the dominant, recessive, homozygote, heterozygote, and allelic models. Regarding the association between rs1800896 SNP and the progression of leishmaniasis, the pooled odds ratios showed no association under all genotype models. Hence, IL-10 SNPs did not show significant association and were not considered a risk factor for the progression of leishmaniasis.
In-resin CLEM of Epon-embedded cells using proximity labeling
Authors: Sanada Takahito , Yamaguchi Junji , Furuta Yoko , Kakuta Soichiro , Tanida Isei , Uchiyama Yasuo 
Published: July 2022

AbstractBiotin ligases have been developed as proximity biotinylation enzymes for analyses of the interactome. However, there has been no report on the application of proximity labeling for in-resin correlative light-electron microscopy of Epon-embedded cells. In this study, we established a proximity-labeled in-resin CLEM of Epon-embedded cells using miniTurbo, a biotin ligase. Biotinylation by miniTurbo was observed in cells within 10 min following the addition of biotin to the medium. Using fluorophore-conjugated streptavidin, intracellular biotinylated proteins were labeled after fixation of cells with a mixture of paraformaldehyde and glutaraldehyde. Fluorescence of these proteins was resistant to osmium tetroxide staining and was detected in 100-nm ultrathin sections of Epon-embedded cells. Ultrastructures of organelles were preserved well in the same sections. Fluorescence in sections was about 14-fold brighter than that in the sections of Epon-embedded cells expressing mCherry2 and was detectable for 14 days. When mitochondria-localized miniTurbo was expressed in the cells, mitochondria-like fluorescent signals were detected in the sections, and ultrastructures of mitochondria were observed as fluorescence-positive structures in the same sections by scanning electron microscopy. Proximity labeling using miniTurbo led to more stable and brighter fluorescent signals in the ultrathin sections of Epon-embedded cells, resulting in better performance of in-resin CLEM.
MICU1 controls spatial membrane potential gradients and guides Ca2+ fluxes within mitochondrial substructures
Authors: Gottschalk Benjamin , Koshenov Zhanat , Waldeck-Weiermair Markus , Radulović Snježana , Oflaz Furkan E. , Hirtl Martin , Bachkoenig Olaf A. , Leitinger Gerd , Malli Roland , Graier Wolfgang F. 
Published: July 2022

AbstractMitochondrial ultrastructure represents a pinnacle of form and function, with the inner mitochondrial membrane (IMM) forming isolated pockets of cristae membrane (CM), separated from the inner-boundary membrane (IBM) by cristae junctions (CJ). Applying structured illumination and electron microscopy, a novel and fundamental function of MICU1 in mediating Ca2+ control over spatial membrane potential gradients (SMPGs) between CM and IMS was identified. We unveiled alterations of SMPGs by transient CJ openings when Ca2+ binds to MICU1 resulting in spatial cristae depolarization. This Ca2+/MICU1-mediated plasticity of the CJ further provides the mechanistic bedrock of the biphasic mitochondrial Ca2+ uptake kinetics via the mitochondrial Ca2+ uniporter (MCU) during intracellular Ca2+ release: Initially, high Ca2+ opens CJ via Ca2+/MICU1 and allows instant Ca2+ uptake across the CM through constantly active MCU. Second, MCU disseminates into the IBM, thus establishing Ca2+ uptake across the IBM that circumvents the CM. Under the condition of MICU1 methylation by PRMT1 in aging or cancer, UCP2 that binds to methylated MICU1 destabilizes CJ, disrupts SMPGs, and facilitates fast Ca2+ uptake via the CM.
Positional cues and cell division dynamics drive meristem development and archegonium formation in Ceratopteris gametophytes
Authors: Geng Yuan , Yan An , Zhou Yun 
Published: July 2022

AbstractFern gametophytes are autotrophic and independent of sporophytes, and they develop pluripotent meristems that drive prothallus development and sexual reproduction. To reveal cellular dynamics during meristem development in fern gametophytes, we performed long-term time-lapse imaging and determined the real-time lineage, identity and division activity of each single cell from meristem initiation to establishment in gametophytes of the fern Ceratopteris richardii. Our results demonstrate that in Ceratopteris gametophytes, only a few cell lineages originated from the marginal layer contribute to meristem initiation and proliferation, and the meristem lacks a distinguishable central zone or apical cell with low division activity. Within the meristem, cell division is independent of cell lineages and cells at the marginal layer are more actively dividing than inner cells. Furthermore, the meristem triggers differentiation of adjacent cells into egg-producing archegonia in a position-dependent manner. These findings advance the understanding of diversified meristem and gametophyte development in land plants.
Pharmacological Treatment of Early-Onset Schizophrenia: A Critical
Review, Evidence-Based Clinical Guidance and Unmet Needs
Authors: Leucht Stefan , Arango Celso , Lopez-Morinigo Javier-David 
Published: July 2022

AbstractEarly-onset schizophrenia (EOS) – onset before age 18 – is linked
with great disease burden and disability. Decision-making for EOS
pharmacological treatment may be challenging due to conflicting information from
evidence and guidelines and unidentified care needs may remain unmet.We searched for systematic reviews, meta-analyses and umbrella reviews of EOS
pharmacological treatment published in PubMed over the past 10 years and
selected five clinical guidelines from Europe, North-America and Australia.
Based on predefined outcomes, we critically compared the evidence supporting
EOS-approved drugs in Europe and/or North-America with guidelines
recommendations. We also evaluated the coverage of these outcomes to identify
unmet needs.One systematic review, nine meta-analyses and two umbrella reviews (k=203
trials, N=81,289 participants, including duplicated samples across
selected articles) were retrieved. Evidence supported the efficacy of
aripiprazole, clozapine, haloperidol, lurasidone, molindone, olanzapine,
quetiapine, risperidone and paliperidone in EOS, all of which obtained approval
for EOS either in Europe and/or in North-America. Cognition, functioning
and quality of life, suicidal behaviour and mortality and services utilisation
and cost-effectiveness were poorly covered/uncovered.Among the antipsychotics approved for EOS, aripiprazole, lurasidone, molindone,
risperidone, paliperidone and quetiapine emerged as efficacious and comparably
safe options. Olanzapine is known for a high risk of weight gain and haloperidol
for extrapyramidal side-effects. Treatment-resistant patients should be offered
clozapine. Future long-term trials looking at cognition, functioning, quality of
life, suicidal behaviour, mortality, services utilisation and cost-effectiveness
are warranted. Closer multi-agency collaboration may bridge the gap between
evidence, guidelines and approved drugs.
Early Seizure Recurrence in Children Admitted for Nonfebrile Seizures in the Emergency Department: A Prospective Study
Authors: Ropars Juliette , Sacaze Elise , Abalea Lydie , Peudenier Sylviane , Vic Philippe , Lemoigno Laurence , Dailland Thomas , Courgeon Lisa , Lefranc Jeremie 
Published: July 2022

Abstract
Objective This study was aimed to determine the rate, timing, and risk factors of acute recurrence of seizures in the children admitted for nonfebrile seizure in the emergency department (ED).
Methods This multicenter prospective study was conducted in the ED of three hospitals. All consecutive visits of children aged 28 days to 15 years who attended the ED for a nonfebrile seizure for 1 year were included in the study and prospectively followed. The rate of acute seizure recurrence within 24 hours was evaluated and association with potential risk factor was tested. Timing of seizure recurrence was assessed.
Results A total of 181 ED visits were enrolled. Overall, 19.9% (36/181) of children presented acute seizure recurrence, 50% of seizure recurrence occurred during the 2 hours after ED arrival and 70% within 6 hours. Multivariable analysis showed that age of <5 years and seizure recurrence in the emergency department were associated with a significant increase in acute recurrence risk.
Conclusion Early seizure recurrence is common in children with nonfebrile seizure, with younger children at higher risk. Based on these findings, acute recurrence risk after a nonfebrile seizure should justify to observe the children admitted for a nonfebrile seizure in the ED, especially young children. A larger study should analyze other risk factors associated with increased risk of acute seizure recurrence and help ED management.
Comparison of Complications between Anterior Cervical Diskectomy and Fusion versus Anterior Cervical Corpectomy and Fusion in Two- and Three-Level Cervical Spondylotic Myelopathy: A Meta-analysis
Authors: Yu Zhentang , Shi Xiaohan , Yin Jianjian , Jiang Xijia , Xu Nanwei 
Published: July 2022

Abstract
Background In this study, we systematically analyze the differences in complications between anterior cervical diskectomy and fusion (ACDF) and anterior cervical corpectomy and fusion (ACCF) in two- and three-level cervical spondylotic myelopathy (CSM).
Methods We performed a systematic search in MEDLINE, EMBASE, PubMed, Web of Science, Cochrane databases, Chinese Biomedical Literature Database, CNKI, and Wan Fang Data for all relevant studies. All statistical analyses were performed using Review Manager version 5.3.
Results A total of 11 articles with 849 study subjects were included, with 474 patients in the ACDF group and 375 patients in the ACCF group. The results of the meta-analysis showed that in C5 palsy (odds ratio [OR]: 0.41; 95% confidence interval [CI]: 0.16–1.06), pseudarthrosis (OR: 1.07; 95% CI: 0.23–5.07), dysphagia (OR: 1.06; 95% CI: 0.60–1.86), infection (OR: 0.41; 95% CI: 0.16–1.09), cerebrospinal fluid leakage (OR: 1.21; 95% CI: 0.39–3.73), graft dislodgment (OR: 0.28; 95% CI: 0.06–1.37), and hematoma (OR: 0.32; 95% CI: 0.06–1.83), there are no significant differences between the ACDF and ACCF groups, whereas total complication (OR: 0.50; 95% CI: 0.31–0.80) showed that the ACDF group had a significantly lower morbidity than the ACCF group. Furthermore, the three-level subgroup of ACDF had significantly better results in C5 palsy (OR: 0.31; 95% CI: 0.11–0.88), infection (OR: 0.22; 95% CI: 0.05–0.94), graft dislodgment (OR: 0.07; 95% CI: 0.01–0.40), and total complication (OR: 0.37; 95% CI: 0.23–0.60) compared with the ACCF subgroup.
Conclusion In general, postoperative pseudarthrosis, dysphagia, cerebrospinal fluid leakage, hematoma, C5 palsy, infection, and graft dislodgment did not differ significantly between the two groups. Total complication was significantly less in the ACDF group compared to the ACCF group. In the three-level subgroup, the morbidity of C5 palsy, infection, and graft dislodgment was significantly lower in ACDF than in ACCF.
Acute Kidney Injury in Pregnancies Complicated by Late-Onset Preeclampsia with Severe Features
Authors: Rodriguez Aldeboran N. , Nelson David B. , Spong Catherine Y. , McIntire Donald D. , Reddy Meghana T. , Cunningham F. Gary 
Published: July 2022

Objectives Acute kidney injury (AKI)-complicating pregnancy is used as a marker of severe maternal morbidity (SMM) and frequently associated with obstetric hypertensive disorders. We examined AKI in pregnancies complicated by late-onset preeclampsia with severe features (SPE) using the Kidney Disease: Improving Global Outcomes (KDIGO) criteria. We compared outcomes of pregnancies with and without AKI and stratified by stage of disease. We further differentiated renal dysfunction at the time of admission and compared outcomes to those who developed AKI after admission.
Study Design This was a retrospective cohort study of women with care established before 20 weeks and diagnosed with preeclampsia with severe features with delivery at ≥34 weeks. Women with chronic hypertension or suspected underlying renal dysfunction were excluded. KDIGO criteria were applied to stratify staging of renal disease. Demographics and perinatal outcomes were compared using Chi-square analysis and Wilcoxon's rank-sum test with p < 0.05 considered significant.
Results From January 2015 through December 2019, a total of 3,515 women meeting study criteria were delivered. Of these, 517 (15%) women met KDIGO criteria for AKI at delivery with 248 (48%) having AKI at the time of admission and the remaining 269 (52%) after admission. Stratified by severity, 412 (80%) had stage 1 disease, 89 (17%) had stage II, and 16 (3%) had stage III. Women with AKI had higher rates of cesarean delivery (risk ratio [RR] = 1.3; 95% confidence interval [CI]: 1.17–1.44), postpartum hemorrhage (RR = 1.46; 95% CI: 1.29–1.66), and longer lengths of stay. Other associated outcomes included NICU admission (RR = 1.72; 95% CI: 1.19–2.48), 5-minute Apgar score ≤ 3 (RR = 5.11; 95% CI: 1.98–13.18), and infant length of stay.
Conclusion Of women with late preterm SPE, 15% were found to have AKI by KDIGO criteria. The majority (80%) of AKI was stage I disease, and approximately half of the cases were present by the time of admission.
Key Points
Supplementation with Cod Liver Oil Capsules Reduces Weight Loss and Mucositis in Head and Neck Cancer Patients Undergoing Curative Radiotherapy without Affecting the Treatment Response
Authors: Hegde Sanath , Rao Suresh , D'souza Rhea Katherine , Baliga Manjeshwar Shrinath 
Published: July 2022

Abstract
Background Weight loss is a common observation in head and neck cancer (HNC) patients and the severity depends on the modalities used. The purpose of this study was to evaluate the effectiveness of providing two capsules of fish oil supplement each day during the course of curative radiotherapy for HNC patients.
Materials and Methods This was a retrospective single-center study, and files of HNC patients treated with radiotherapy between the months of January 2015 and March 2015 were evaluated. Data on gender, age, tumor, treatment details, adverse effects, weight before and at the end of the treatment, and treatment response were obtained from the patient files. The data collected were entered into Excel sheet and subjected to statistical analysis using chi-square tests, unpaired t-test, and analysis of variance with post hoc Tukey test. A p-value of <0.05 was considered significant.
Results Records of 68 patients treated during the study period with radiation for HNC and admitted to the inpatient facility throughout the treatment period were retrospectively reviewed. Majority of the patients had advanced stage tumors. There was no difference in the initial weight of the patients in the two groups while a significant difference was seen in the final weight (p = 0.007). The number of patients with severe weight loss (>5 kg) was more in the control than in the fish oil cohort (68.89 vs. 43.48) and was significant (p = 0.042). In addition to this, the incidence of mucositis was delayed and also lesser in severity in the cohorts that had received fish oil. At the dose used, fish oil capsules did not have any adverse effects and importantly there was no significant difference in treatment response.
Conclusion The results of the study indicate that administering fish oil capsules was effective in arresting weight loss and delaying and mitigating mucositis in HNC patients undergoing curative radiotherapy. Fish oil capsule has good safety profile, was devoid of any toxic effects, and has a good clinical application value.
Cognitive corpus linguistics and pedagogy
Authors: Gilquin Gaëtanelle 
Published: July 2022

AbstractThis article deals with applied cognitive corpus linguistics, that is, the combined use of cognitive linguistics and corpus linguistics for pedagogical purposes. It describes the main features of each framework in terms of teaching applications and underlines their complementarity. Through several illustrations, it shows how applied cognitive corpus linguistics combines the benefits of corpus data (authenticity, quantification, diversification of genres, access to learner production, etc.) with those of cognitive principles (linguistic motivation, enhanced salience, realignment of form and meaning, etc.) in ways that are expected to foster the development of the learner’s language system.
Air pollution and climate change impact on forest ecosystems in Asian region – a review
Authors: Sonwani Saurabh , Hussain Sharfaa , Saxena Pallavi 
Published: July 2022

ABSTRACT

Forests are complex ecosystems comprising various trophic levels responsible for carrying out various biogeochemical processes and providing ecosystem services. However, forests in Asia are doubly challenged by climate change and air pollution. The rapidly changing air quality, with increasing concentration of greenhouse gases (GHGs), trace gases, volatile organic compounds (VOCs), and ozone (O
3
) also causes global warming leading to climate change, thus jointly creating a challenging condition for the forest ecosystem. The impact on forest ecosystems of the two anthropogenic stressors, viz., climate change and air pollution, requires global attention. These two stressors have been widely studied separately but their combined impact on the forest ecosystem has not been studied extensively, particularly in the Asian region. In this review article, we attempt to explore the importance of interlinking air pollution and climate change impact on Asian forests, by studying the decline of different forest types as a background and markers of forest ecosystem degradation. Our main aim is to understand and summarise the past and ongoing research in this area and to facilitate researchers and policymakers to upgrade their research, policies, and management strategies in the area of integration of air pollution and climate change impact on forest ecosystems in the Asian region.
On gravitational preheating
Authors: Lebedev Oleg , Yoon Jong-Hyun 
Published: July 2022

Abstract
We consider dark matter production during the inflaton
oscillation epoch. It is conceivable that renormalizable
interactions between dark matter and inflaton may be negligible. In
this case, the leading role is played by higher dimensional
operators generated by gravity and thus suppressed by the Planck
scale. We focus on dim-6 operators and study the corresponding
particle production in perturbative and non-perturbative
regimes. We find that the dark matter production rate is dominated
by non-derivative operators involving higher powers of the inflaton
field. Even if they appear with small Wilson coefficients, such
operators can readily account for the correct dark matter
abundance.