bluuebunny/crossref_metadata_2025_split huggingface dataset:
A retrospective analysis and clinical application value of the expression levels of high mobility group protein B1, β1 globulin and Toll-like receptor 4 in serum of children with rheumatoid arthritis
Authors: Wang Wei , Li Yuanyuan , He Feng 
Published: November 2022

Abstract
Methods
50 healthy children are selected as the healthy control group during the same period. The molecular levels of serum HMGB family proteins in the children are detected by Enzyma-Linked Immunosorbent Assay (ELISA), and flow cytometry is used to detect the levels of serum Toll-like receptors and their expression levels.
Results
The classification and expression levels of serum globulin are detected by automatic biochemical analyzer. By comparison of the expression levels of serum HMGB1, β1 globulin and TLR-4, the correlation between the expression levels of the above factors and the incidence of disease is analyzed. The experimental results show that expression levels of serum HMGB1, β1 globulin and TLR-4 in the study group are significantly higher than those in the control group (P < 0.05).
Conclusion
It is clearly evident that Serum HMGB1, β1 globulin and TLR-4 are highly expressed in the serum of children with JIA, and are positively correlated with the occurrence of the disease.
The efficacy of an oral probiotic associated with a fixed combination of benzoyl peroxide and adapalene in the treatment of acne: A randomized, double-blind, placebo-controlled clinical trial.
Authors: Rocha Marco Alexandre Dias da , Schalka Sergio , Bayan Fernanda Calvente , Yoshizumi Thais Helena C. B. Matsuda , Blum Vinicius Fontanesi 
Published: November 2022

Abstract
Objective: Clinical trial carried out to evaluate the superiority of efficacy of a treatment regimen with an oral probiotic associated with a fixed combination of 0.1% adapalene and 2.5% benzoyl peroxide compared to an oral placebo with the same topical treatment in patients with mild to moderate acne.
Patients and Methods: This was a randomized, double-blind, placebo-controlled study conducted with 212 patients aged from 12 to 35 years; 107 in the test treatment arm and 105 in the comparator arm. The study was divided into two phases of 90 days each, totaling 180 days. In the first phase, patients received treatment with 2.5% benzoyl peroxide and 0.1% adapalene associated with a probiotic (IT), or 2.5% benzoyl peroxide and 0.1% adapalene associated with placebo (CT). In the second phase (90 days), patients received only oral treatment with a probiotic or placebo. Efficacy criteria were: reduction of the Investigator Global Assessment (IGA) scale to 0 or 1, and reduced lesion count.
Results: There was a significant difference in the proportion of participants with IGA 0 or 1 – the arm receiving the test treatment was superior to the comparator group at all study time points (30, 60, 90, 120, 150 and 180 days) (p<0.05). Regarding lesion counts, the test arm was superior, although this difference was not statistically significant. Both treatments were safe and well tolerated.
Conclusion: In light of the evidence on efficacy and safety, treatment with probiotics should be considered as an adjuvant therapy for acne control.
Characterisation of Staphylococcus species from subclinical mastitis dairy cattle in Free State Province, South Africa
Authors: Khasapane Ntelekwane George , Thekisoe Molifi Matlhahane Oriel , Khumalo Zamantungwa Thobeka Happiness , Koos Myburgh , Nkhebenyane Sebolelo Jnae 
Published: November 2022

Abstract
Background: Mastitis is an important disease in dairy industry causing major economic burden. Subclinical mastitis (SCM) is regarded as one of the most economically important diseases affecting the dairy industry due to the financial constraints incurred by it. Subclinical mastitis as compared to clinical mastitis does not cause visible changes in the udder or physical changes of the milk hence the need for routine testing and surveillance.
Materials and methods: The aim of this study was to assess the prevalence of subclinical mastitis in dairy cows of small-holder farms in the Free State province using California mastitis test (CMT) and somatic cell counts (SCC) and further characterise staphylococcal species isolated by using Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and Polymerase chain reaction (PCR).
Results: The CMT results revealed that out of 166 cows, only 55 (33%) were positive for intramammary infection. The quarter level prevalence based on SCC was 87 (54%) for intramammary infections. Out of 50 bacterial culture isolates, 48 (96%) were identified as S. aureus (76%), S. epidermidis (4%), S. chromogenes (12%) and S. haemolyticus (4%) by MALDI-TOF MS. The 16S rDNA PCR products and obtained sequences indicated that S. aureus was the dominant species infecting tested cows at 76%, followed by S. chromogenes, S. agnetis, S. argenteus and S. devriesei at 10%, 4%, 4%) and 1% respectively.
Conclusions: This study has shown the presence of diverse Staphylococcusspecies infecting subclinical mastitis cows in sampled areas of the Free State province. It further indicated the importance of using various diagnostic tools for bacterial infection surveillance involved in subclinical mastitis to ensure accurate species identification.
LncRNA BCAR4 promotes migration, invasion, and chemo-resistance by inhibiting miR-644a in breast cancer
Authors: wu tangwei , Li xiaoyi , yan ge , Tan zheqiong , Zhao dan , Liu shuiyi , Wang hui , xiang yuan , Chen Weiqun , Lu Hongda , Liao Xinghua , Li yong , Lu Zhongxin 
Published: November 2022

Abstract
Background
Metastasis and drug resistance of breast cancer have become a barrier to treating patients successfully. Long noncoding RNAs (lncRNAs) are known as vital players in cancer development and progression.
Methods
The RT-qPCR were used to detect the gene expression. Colony formation assay, would healing assay, and transwell assay were performed to investigate oncogenic functions of cells. CCK8 assay was used to detect the cell vability. Western blot was applied to detect the protein level. Dual-luciferase reporter assay was used to determine the relationship between molecules. Mouse orthotopic xenograft tumor models were established to evaluate the effects of BCAR4 on tumor growth and metastasis in vivo.
Results
LncRNA BCAR4 was significantly increased in breast cancer patients’ tissues and plasma and upregulated in breast cancer cell lines. BCAR4 upregulation was correlated with the TNM stages and decreased after surgical removal of breast tumors. Silencing of BCAR4 suppressed breast cancer cell colony formation, migration, invasion, and xenograft tumor growth and promoted chemo-sensitivity. Mechanistically, BCAR4 facilitates breast cancer migration and invasion via the miR-644a-CCR7 axis of the MAPK pathway. BCAR4 promotes ABCB1 expression indirectly by binding to and down-regulating miR-644a to induce chemo-resistance in breast cancer.
Conclusions
Our findings provide insights into the oncogenic role of BCAR4 and implicate BCAR4 as a potential diagnostic biomarker and a promising therapeutic agent to suppress metastasis and inhibit chemo-resistance of breast cancer.
Novel Ferroptosis-related gene signature for predicting the prognosis of breast cancer
Authors: Xu Jinwen , Guo Yan , Cui Yuqing , Chen Fengzhi , Li Yangyang , Yao Guanyu , Chen Xuesong 
Published: November 2022

AbstractBackground: Ferroptosis is a novel form of regulated cell death, however, the potential functions of ferroptosis in the breast cancer (BRCA) prognosis, immune infiltration, and drug resistance remain unknown. Methods: In this study, the characteristic patterns of ferroptosis in estrogen receptor–positive (ER+) breast cancer were systematically investigated with the interactions between ferroptosis and the tumor microenvironment (TME). On the mRNA expression profiles of 56 ferroptosis-related genes (FRGs), two ferroptosis patterns were constructed, with distinct prognosis, immune cell infiltrations and tamoxifen resistance. Results: The high ferroptosis scores were characterized by better prognosis, increased immune cell infiltration, higher immune and stromal scores, lower tumor purity, lower tumor mutational burden (TMB) and better Tamoxifen response, immune-related pathways showed statistically significant activation, while pathways related to cell repair and metabolism showed significant inhibition. Conclusion: Collectively, ferroptosis could be involved in the diverse and complex TME. Evaluation of the ferroptosis patterns may heighten the comprehension about immune infiltrations in the TME, ferroptosis-related risk score model can predict the prognostic of ER + BRCA patients and for the treatment of resistance to tamoxifen administration and assisting oncologists to generate individualized immunotherapeutic strategies.
Methodology for determination of contrast agent relaxivity using MRI
Authors: Reyes-Molina Israel , Hernández-Rodríguez Armando , Cabal-Mirabal Carlos , Gonzalez-Dalmau Evelio 
Published: November 2022

Abstract
Knowledge of the longitudinal and transverse relaxivities (r1 and r2) of a contrast agent (CA) is essential for its magnetic characterization. These parameters can be measured using Magnetic Resonance Imaging (MRI) clinical scanners with the advantage of characterizing the CA under the same experimental conditions where it will be employed. Nevertheless, when using MRI, there are several limitations to be considered, and we provide ways to compensate for them to obtain more accurate results. Here, we present a fast and robust methodology to determine the relaxivity of CA solutions using a 3T MRI clinical scanner with a birdcage coil. Relaxivity measurements were performed on a phantom consisting of 5 samples of copper sulfate at different concentrations. We optimized image acquisition for total scan time using three different pulse sequences. Post-processing steps following image acquisition were implemented in a semiautomatic MATLAB toolbox. Relaxation times were estimated using the three-parameter model with the Levenberg-Marquardt algorithm. Statistical comparisons demonstrate good reproducibility and robustness in the relaxivity estimation by each method.
Exploring the changes of perceptual eye position after SMILE based on the virtual reality platform
Authors: Luo linbi , kong Xiangmei , Huang Junting , Guo Lu , Lv Hongbin 
Published: November 2022

Abstract
OBJECTIVE: To investigate the pattern of changes in perceptual eye position in patients after Femtosecond Laser Small Incision Lenticule Extraction(SMILE), which was examined using the visual perception detection system developed by the National Engineering and Technology Research Center for Healthcare Appliances.
METHODS: A total of 141 patients participated in this prospective study. The patients were divided into three groups according to the degree of myopia: low myopic group
( equivalent spherical refraction ≤ 3.00D), moderate myopic group(3.00D < equivalent spherical refraction ≤ 6.00D), and high myopic group (equivalent spherical refraction > 6.00D). The relationship of perceptual eye position was measured by the computer-controlled visual perception detection system before, 1 day, 1 week and 1 month after surgery to investigate the changes of perceptual eye position after SMILE.
RESULTS: The differences in horizontal perceptual eye position were statistically significant at all time periods (X2=82.004, P<0.001), and the horizontal perceptual eye position improved at 1 day and 1 week postoperatively compared with that before surgery, but rebounded in January postoperatively. The differences in vertical perceptual eye position were statistically significant at all time periods (X2=38.59, P<0.001), and the vertical PEP improved at 1 day, 1 week, and 1 month postoperatively compared with the preoperative level, but the improvement was small. The difference between horizontal perceptual eye position and vertical perceptual eye position in all three groups was not statistically significant, and it can be concluded that the deviation of perceptual eye position after SMILE is not related to the degree of myopia.
CONCLUSION: Most myopic patients have perceptual eye position shift before refractive correction, and SMILE surgery can improve the perceptual eye position shifts significantly in the early postoperative period, but the long-term results need to be further observed.
Efficacy of salvage therapies after failure of adjuvant anti- PD-1 monotherapy for melanoma in Chinese population: A multi-institutional cohort study
Authors: Jia Dong-Dong , Xu Yu , Li Ting , Yang Ji-Long , Chen Yong , Li Tao 
Published: November 2022

Abstract
Background
The majority of melanoma patients experienced relapse during the adjuvant therapy or after the end of the therapy.
Method
A total of sixty-one patients from 3 melanoma centres who recurred having received adjuvant pembrolizumab for resected stage III/IV melanoma were enrolled. Disease characteristics, recurrence characteristics, subsequent management and outcomes were noted.
Result
A total of sixty-one patients were enrolled in this study. Median time to first relapse from commencement of adjuvant pembrolizumab was 8 months (1–22 months). First recurrences were locoregional alone in 25 (41%), distant alone in 29 (47.5%) and concurrent locoregional and distant relapse in 7 (11.5%). 3 (60%) patients treated with adjuvant pembrolizumab following surgery, 2 (100%) patients treated with adjuvant chemotherapy, 2 (66.7%) patients treated with adjuvant chemotherapy and pembrolizumab combined and 3 (100%) patients treated with adjuvant radiotherapy and pembrolizumab combined had further recurrence. Of three patients treated with adjuvant BRAF/MEKi following first relapse, none have yet recurred. Of 8 patients treated with pembrolizumab alone, only one patient (12.5%) who recurred after ceasing adjuvant PD1 had a partial response. The overall response rate to BRAF/MEKi was 75%, 3/4, to pembrolizumab in combination with an oral multi-targeted receptor tyrosine kinase inhibitor was 22.2%, 2/9, to chemotherapeutic agents alone was 33.3%, 1/3 and to chemotherapeutic agents combined with pembrolizumab was 37.5%, 3/8. The patient treated with imatinib had progressive disease after 3 months of treatment. Of 6 patients who received temozolomide combined with pembrolizumab, 3 (3/6, 50%) had a partial response. The median OS of patients who relapsed locoregionally only was longer than patients who relapsed distally at first recurrence (35 months and 14 months, respectively; P < 0.01).
Conclusion
Outcomes of patients with disease recurrence during or after completion of 1-year adjuvant anti-PD1 were poor despite multimodality treatment.
A multi-responsive diarylethene chemosensor via salicylaldehyde linking fluorescein unit
Authors: Li Xue , Ai Yin , Kang Huimin , Fan Congbin , Liu Gang , Ding Haichang , Pu Shouzhi 
Published: November 2022

Abstract
A novel multi-responsive diarylethene derivative (1O) by linking a fluorescein group has been synthesized. Colorless 1O not only displayed good photochromic property to form cyclization state 1C, but also the 1C having multi-responsive characteristic to Cu2+ and basic stimulations. After Cu2+ was introduced, 1C showed excellent absorption and fluorescent spectra changed upon binding with Cu2+. The color of 1C solution turned from purple to colorless and the corresponding fluorescence color changed from dark to yellow, and the binding stoichiometry of 1C to Cu2+ was 1:1 and the detection limit was 0.11 µM. Moreover, 1C displayed distinctly reversible change on absorption and fluorescent spectra in basic environment, the basic environment can discolor the solution and turn on the fluorescence effect, suggesting that 1C can be used as pH sensor.
Association between arteriosclerosis and the risk of falls in the elderly
Authors: Zhang Kexin , Ju Yanmin , Yang Di , Cao Mengyu , Liang Hong , Leng Jiyan 
Published: November 2022

Abstract
Objective: To study the risk factors of falls in the elderly and explore the correlation between arteriosclerosis and the risk of falls in the elderly.
Method: This is a cross-sectional study. The subjects were inpatients ≥ 60 years old in the cadre ward of the first hospital of Jilin University from May 1, 2019 to December 31, 2020. The following information was obtained from the past comprehensive geriatric assessment database of the cadre ward. Ankle brachial index (ABI) and ankle brachial pulse wave velocity (baPWV) were measured by OMRON arteriosclerosis detection device. We assessed according to the internationally used fall risk assessment tool (FRA), ≤ 2 points are divided into low-risk group, 3 - 9 points are divided into medium-risk group, and ≥ 10 points are divided into high-risk group.
Results: There were significant differences among the three groups in age, weight, educational background, low gait speed, cognitive impairment, malnutrition, ability of daily living, depression (P < 0.05). The baPWV and ABI were higher in high-risk group than in low-risk group; The baPWV in high-risk group was higher than that in medium risk group and low-risk group. For each additional unit of baPWV, the probability of the fall risk increasing by one level is 1.701 times higher than the original (OR= 1.701, 95%CI: 0.533-11.822, P= 0.001). For each additional unit of ABI, the probability of the fall risk increasing by one level is 1.976 times higher than the original (OR= 1.976, 95%CI: 1.213-3.219, P= 0.006).
Conclusion:The increase of baPWV and the decrease of ABI are closely related to the increased risk of falls in the elderly.
Extensive Reading Affects Second Language Listening Proficiency: an fNIRS Study
Authors: Chiba Katsuhiro , Miyazaki Atsuko , Yokoyama Satoru 
Published: November 2022

Abstract
One of the difficulties in learning English as a foreign language (EFL) is obtaining sufficient input in the language. The importance of input in EFL settings has been discussed for decades, and extensive reading (ER) has been proposed to supply input to learners of English. Many studies have provided evidence that ER enhances learners' reading proficiency, vocabulary, and grammar. Interestingly, some studies have reported the effect of ER on listening besides reading skills. The current study aimed to clarify the effect of ER on listening ability from both behavioral and neurophysiological points of view. The participants were university students in Japan who took the same compulsory English class and were required to complete 150,000 words of ER per semester. To examine the effect of ER, measurements were obtained at three time points: the beginning of the spring semester, the end of the spring semester, and the end of the fall semester. Our results showed that ER training affects both behavioral performance and brain activity during foreign language listening and that ER training enables listening processing with less energy. The findings demonstrate that ER training affects real-time processing during foreign language listening tasks.
Flexible process planning based on predictive models for Machining time and energy consumption
Authors: Chu Hongyan , Dong Ke , Yan Jun , Li Zhuoran , Liu Zhifeng , Cheng Qiang , Zhang Caixia 
Published: November 2022

Abstract
Machining time and energy consumption are common process planning evaluation metrics. However, it is difficult to obtain accurate machining time and energy consumption values in the process planning stage, which may result in obtaining process routes that are not optimal. To solve these problems, this paper proposes a flexible process planning method based on processing time and energy consumption prediction models. Since there are many factors affecting processing time and energy consumption in discrete workshops, the main factors affecting processing time and energy consumption are analyzed based on historical workshop processing data, a processing time and energy consumption prediction model for process planning is established, and an RBF neural network is designed for prediction. Considering the flexibility of multiple processes, a mathematical model of the flexible process planning problem based on the machining time and energy consumption prediction model was established. A multi-objective algorithm using a multidimensional real number coding method is proposed for problem solving with the completion time and energy consumption minimization as the optimization objective. Finally, simulation experiments are conducted to verify the validity of the prediction model based on the data obtained from the analysis of a mold manufacturing plant. Meanwhile, the best performance of the SPEA2 algorithm with parameters {crossover rate: 0.8, mutation rate: 0.8} was determined through comparative analysis by combining two common multi-objective algorithms, SPEA2 and NSGA-II, with a machined part as an example, and the effectiveness and practicality of the proposed process planning model was verified.
Association between seasonal factors and severity of obstructive sleep apnea-hypopnea syndrome in children
Authors: xu xueyun , He yanyu , Jiang Yuting , Lv Meng , Geng Yaxuan , Wang Zhihui , Zhang Zhen , Wang Fengqian , Wang Shuqi , Zhu Huie , Wang Yuqing 
Published: November 2022

Abstract
Background
Limited data are available on the relationship between seasonal factors and obstructive sleep apnea-hypopnea syndrome (OSAHS) in China. The aim of this study was to investigate the effects of seasonal factors on the severity of OSAHS in children.
Methods
Children presenting with snoring and/or mouth breathing, suspected of having OSAHS, and admitted to the Department of Respiratory Medicine of the Children's Hospital of Soochow University from December 2016 to February 2022 were enrolled in this study. The severity of OSAHS was determined by full-night polysomnography (PSG) in the sleep laboratory. The correlation between seasonal factors and PSG monitoring results was analyzed.
Results
Of the 589 included patients, 301 cases (51.1%) were diagnosed with OSAHS. Among them, 77 cases (25.6%) were detected in spring, 74 cases (24.6%) were detected in summer, 59 cases (19.6%) were detected in autumn, and 91 cases (30.2%) were detected in winter. There were 238 cases (79.1%) of tonsillitis hypertrophy, 276 cases (91.7%) of adenoid hypertrophy, 215 cases (71.4%) of rhinitis/sinusitis, and 36 cases (12.0%) of asthma. The proportion of rhinitis/sinusitis and tonsillar hypertrophy in spring was higher than that in summer (P = 0.015 and 0.036, respectively), and the proportion of adenoid hypertrophy in winter was higher than that in summer (P = 0.024). The diagnostic rate of severe OSAHS in winter was higher than that in summer (χ2 = 7.053, P = 0.008). The respiratory arousal index in winter was higher than that in summer (H = -39.297, P = 0.018), and the spontaneous arousal index in spring was higher than that in autumn (H = 44.059, P = 0.020). It was found that the rapid eye movement (REM)-apnea index (AI) in spring and winter was higher than that in summer (H = 52.292, -52.554; all P < 0.05). The comparison of PSG results of children with OSAHS under the age of 7 years in different seasons showed that the respiratory arousal index in winter was higher than that in summer (H = -35.570, P = 0.008). The REM-AI in winter and spring was higher than that in summer and autumn, respectively (H = -33.929 and − 38.632; H = 30.665 and 35.368, all P < 0.05).
Conclusion
Severe OSAHS is more common in winter. Respiratory events are increased in spring and winter, mainly in preschool children. Seasonal factors may have a certain impact on OSAHS.