bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial induction of chromosome aneuploidy in CHO cells alters their function as host cells
Authors: Yamano‐Adachi Noriko , Nakanishi Yuto , Tanaka Wataru , Lai YuanShan , Yamazaki Masahiro , Zenner Laura , Hata Hirofumi , Omasa Takeshi 
Published: November 2022

AbstractChinese hamster ovary (CHO) cells are major host cells for biopharmaceuticals. During culture, the chromosome number of CHO cells alters spontaneously. Here, we investigated the effects of artificial changes in the chromosome number on productivity. When cell fusion between antibody‐producing CHO‐K1‐derived cells was induced, we observed a wide range of aneuploidy that was not detected in controls. In particular, antibody productivities were high in clone‐derived cell populations that retained a diverse chromosome number distribution. We also induced aneuploid cells using 3‐aminobenzamide that causes chromosome non‐disjunction. After induction of aneuploidy by 3‐aminobenzamide, cells with an increased chromosome number were isolated, but cells with a decreased chromosome number could not be isolated. When antibody expression vectors were introduced into these isolated clones, productivity tended to increase in cells with an increased chromosome number. Further analysis was carried out by focusing on clone 5E8 with an average chromosome number of 37. When 5E8 cells were used as host, the productivity of multiple antibodies, including difficult‐to‐express antibodies, was improved compared with CHO‐K1 cells. The copies of exogenous genes integrated into the genome were significantly increased in 5E8 cells. These findings expand the possibilities for host cell selection and contribute to the efficient construction of cell lines for recombinant protein production.
Lingular division of the left lung: Only superior and inferior bronchi?
Authors: Zhang Min , Ge Ming‐jian 
Published: November 2022

AbstractThe lingular segment bronchi usually bifurcate into superior lingular (B4) and inferior lingular (B5) segmental bronchi. We report 32 horizontally bifurcated lingular segment bronchi cases, analyzing the bronchovascular variations and the perioperative outcomes. All patients with pulmonary lesions underwent three‐dimensional computed tomography bronchography and angiography between January 2019 and January 2022. The bronchovascular patterns were analyzed for each patient. A consecutive 5280 patients were included, and 32 cases of this malformation were identified. The incidence of the lateral (B4) and medial (B5) lingular segmental bronchi is 0.6%. The A4 and A5 arise separately in 21 (65.6%) patients. In 9 (28.1%) patients, the A4a arises from the common trunk of A4 + 5. In 2 (6.3%) patients, the A4 arises from the interlobe artery, while A5 is a mediastinal lingular artery. The common trunk of V4a + b is found in 19 (59.4%) patients. The V5 is the least changeable of the segmental bronchi veins. Among the 32 patients, five patients had an LS4 segmentectomy. The surgical details of the five patients are summarized. The laterally and medially bifurcated lingular bronchus pattern is a rare anomaly. It implies that the lingular bronchus is not only superiorly and inferiorly bifurcated.
APOBEC3A/B deletion polymorphism and endometrial cancer risk
Authors: Sofiyeva Nigar , Krakstad Camilla , Halle Mari K. , O'Mara Tracy A. , Romundstad Pål , Hveem Kristian , Vatten Lars , Lønning Per E. , Gansmo Liv B. , Knappskog Stian 
Published: November 2022

AbstractBackgroundA common 30 kb deletion affecting the APOBEC3A and APOBEC3B genes has been linked to increased APOBEC activity and APOBEC‐related mutational signatures in human cancers. The role of this deletion as a cancer risk factor remains controversial.Materials and MethodsWe genotyped the APOBEC3A/B deletion in a sample of 1,470 Norwegian endometrial cancer cases and compared to 1,918 healthy controls. For assessment across Caucasian populations, we mined genotypes of the SNP rs12628403, which is in strong linkage disequilibrium with the deletion, in a GWAS dataset of 4,274 cases and 18,125 healthy controls, through the ECAC consortium.ResultsWe found the APOBEC3A/B deletion variant to be significantly associated with reduced risk of endometrial cancer among Norwegian women (OR = 0.75; 95% CI = 0.62–0.91; p = 0.003; dominant model). Similar results were found in the subgroup of endometrioid endometrial cancer (OR = 0.64; 95% CI = 0.51–0.79; p = 3.6 × 10−5; dominant model). The observed risk reduction was particularly strong among individuals in the range of 50–60 years of age (OR = 0.51; 95% CI = 0.33–0.78; p = 0.002; dominant model). In the different populations included in the ECAC dataset, the ORs varied from 0.85 to 1.05. Although five out of six populations revealed ORs <1.0, the overall estimate was nonsignificant and, as such, did not formally validate the findings in the Norwegian cohort.ConclusionThe APOBEC3A/B deletion polymorphism is associated with a decreased risk of endometrial cancer in the Norwegian population.
An empirical investigation of regional differences in consumption behaviors in an emerging economy
Authors: Geiger‐Oneto Stephanie , Nguyen Hieu P. , Munch James 
Published: November 2022

AbstractWidely regarded as one of the most dynamic economies in Southeast Asia, Vietnam has increasingly attracted scholarly interest from diverse business disciplines. However, previous marketing research on Vietnam largely treats the country as a homogenous society. We draw on the literature in sociology, geography, history and predict that significant regional differences exist in consumers' consumption behaviors between the North and South of Vietnam, bearing important implications for international marketers. Using secondary survey data collected by an international market research firm, we show that consumers in Hanoi (HN) and Ho Chi Minh City (HCMC) differ greatly in how they perceive the impact of an economic crisis, how they change their consumption behaviors as a result, and whether they will cut back on spending on themselves, their families, and children. Our results also demonstrate that HN and HCMC consumers differ in their attitude toward luxury product consumption, attitude toward advertising, and marketing versus non‐marketing controlled sources of information. Our research provides important implications for both managers and researchers interested in this fast‐growing economy.
A Multiplex Assay to Assess the Transaminase Activity toward Chemically Diverse Amine Donors
Authors: Czarnievicz Nicolette , Rubanu Maria Grazia , Iturralde Maialen , Albarran‐Velo Jesús , Diamanti Eleftheria , Gotor‐Fernandez Vicente , Skolimowski Maciej , López‐Gallego Fernando 
Published: November 2022

AbstractThe development of methods to engineer and immobilize amine transaminases (ATAs) to improve their functionality and operational stability is gaining momentum. The quest for robust, fast, and easy‐to‐use methods to screen the activity of large collections of transaminases, is essential. This work presents a novel and multiplex fluorescence‐based kinetic assay to assess ATA activity using 4‐dimethylamino‐1‐naphthaldehyde as an amine acceptor. The developed assay allowed us to screen a battery of amine donors using free and immobilized ATAs from different microbial sources as biocatalysts. As a result, using chromatographic methods, 4‐hydroxybenzylamine was identified as the best amine donor for the amination of 5‐(hydroxymethyl)furfural. Finally, we adapted this method to determine the apparent Michaelis‐Menten parameters of a model immobilized ATA at the microscopic (single‐particle) level. Our studies promote the use of this multiplex, multidimensional assay to screen ATAs for further improvement.
Enhancing the Resistive Switching Behavior of WORM Memory Devices Using D−π−A Based Ester‐Flanked Quinolines**
Authors: Angela Varghese M. , Harshini Deivendran , Anjali Anshika , Imran Predhanekar M. , Bhuvanesh Nattamai S. P. , Nagarajan Samuthira 
Published: November 2022

AbstractDonor‐Acceptor systems are highly appreciated in the field of organic memory devices due to their efficient charge transport within the systems. In this work, we have designed and synthesized a D−π−A system constituting ester‐flanked quinolines and functionalized triarylamines (TAA) through a single‐step cross‐coupling reaction to fabricate memory devices via Write‐Once Read‐Many times (WORM) non‐volatile memory. Structure‐property relationships are reconnoitered for these conjugated D−π−A systems through a series of UV, fluorescence, XRD, DFT, and memory characterizations. The UV and CV data show efficient charge transfer with intramolecular charge transfer occurring at 407–417 nm and a short band gap of 2.56–2.65 eV. An enhancement in the resistive switching behavior of the memory devices is observed for the compounds with simple TAA‐quinoline and tert‐butylphenyl substituted TAA and fluorophenyl substituted quinoline due to balanced charge distribution in the compounds. This enhanced switching induces an on/off ratio of 103 by generating a highly ordered arrangement in the thin films. The HOMO, LUMO levels, and the ESP images together estimate a charge transfer and charge trapping as the plausible mechanism for the solution‐processable WORM memory devices. The longer retention time (103 s) and lower threshold voltages (−1.21–−2.12 V) of the devices makes them intriguing compounds for memory applications.
Analysis of user electricity consumption behavior based on density peak clustering with shared neighbors and attractiveness
Authors: Li Qingpeng , Wang Gang , Zhang Yang , Yang Qin 
Published: November 2022

SummaryUser behavior analysis is the research foundation of power load forecasting and power abnormal detection, and it is the theoretical support for smart grid planning and the construction of energy internet. Aiming at the complex characteristics of high‐dimensional, noisy, and multi‐redundant of power load data, this article used the principal component analysis (PCA) to reduce the dimensionality of power data. The density peaks clustering algorithm with shared neighbor and attractiveness (DPC‐SNA) was then used to cluster the data with reduced dimensionality to extract the user's electricity consumption characteristics. The DPC‐SNA algorithm first constructs a sample similarity measurement criterion that shares the similarity of neighbors, on which the local density is defined accordingly. The new local density can effectively distinguish the contributions of local samples and global samples. Incorporating the idea of universal gravitation, a new calculation method of sample attractiveness was defined, and the remaining samples were allocated by the attractiveness matrix. Experiment was performed using the actual load data of special transformer users in a certain area. The results show that there were five typical electricity consumption behavior characteristics in this area, namely, the evening‐peak production type, all‐day production type, multi‐peak production type, daytime production type, and night production type. The corresponding load peak time periods were also obtained.
Connecting epicardial adipose tissue and heart failure with preserved ejection fraction: mechanisms, management and modern perspectives
Authors: van Woerden Gijs , van Veldhuisen Dirk J. , Westenbrink B. Daan , de Boer Rudolf A. , Rienstra Michiel , Gorter Thomas M. 
Published: November 2022

AbstractObesity is very common in patients with heart failure with preserved ejection fraction (HFpEF) and it has been suggested that obesity plays an important role in the pathophysiology of this disease. While body mass index defines the presence of obesity, this measure provides limited information on visceral adiposity, which is probably more relevant in the pathophysiology of HFpEF. Epicardial adipose tissue is the visceral fat situated directly adjacent to the heart and recent data demonstrate that accumulation of epicardial adipose tissue is associated with the onset, symptomatology and outcome of HFpEF. However, the mechanisms by which epicardial adipose tissue may be involved in HFpEF remain unclear. It is also questioned whether epicardial adipose tissue may be a specific target for therapy for this disease. In the present review, we describe the physiology of epicardial adipose tissue and the pathophysiological transformation of epicardial adipose tissue in response to chronic inflammatory diseases, and we postulate conceptual mechanisms on how epicardial adipose tissue may be involved in HFpEF pathophysiology. Lastly, we outline potential treatment strategies, knowledge gaps and directions for further research.
Voltammetric Determination of Marbofloxacin at Carbon Paste Sensor Integrated with Copper Oxide Nanoparticles
Authors: Hendawy Hassan A. , Khaled Elmorsy , Radowan Abdellatef 
Published: November 2022

AbstractThe present work introduced copper oxide nanoparticles as an efficient electrode modifier for sensitive adsorptive differential pulse voltammetric assaying of marbofloxacin (MRB) in pharmaceutical formulations and surface water samples. In the presence of sodium dodecyl sulphate (SDS) at pH 4.0, the marbofloxacin molecule was irreversibly oxidized at the electrode surface showing an anodic oxidation peak at 0.954 V. The electrode reaction mechanism was assumed as adsorption‐reaction controlled accompanied by the transferring of two electrons and proton exchange in agreement with the molecular orbital calculations performed on MRB molecule suggesting the oxidation of the amino group in the piperazinyl ring. At the optimized measuring conditions, the recorded peak heights were linearly correlated with the MRB concentration within the range from 6.67 to 360 ng mL−1, and the estimated LOD value was 2.2 ng mL−1. The integrated sensor showed a prolonged operational lifetime with good reproducibility of measurements. Based on the selectivity and sensitivity of the proposed method, MRB was successfully assayed in pharmaceutical formulations and surface water samples with mean average recoveries agreeable with the official method.
A new species of Scutellaria L. (Lamiaceae) from Iran
Authors: Jamzad Ziba , Mehrnia Mohammad 
Published: November 2022

AbstractA comprehensive study on Scutellaria materials collected in Iran revealed an undescribed species of the genus, belonging to section Apeltanthus which was not previously reported from Iran. The new species is morphologically most similar to S. leptosiphon and S. stocksii distributed in Afghanistan, Pakistan, and Central Asia. The occurrence of a member of section Apeltanthus in Iran shows a disjunct distribution pattern of the species of this section. Scutellaria lurestanica is described as a new species from Iran and is compared with the most similar species. Illustrations of the new species, a distribution map, and a table of diagnostic characters against similar species are provided.
Contrasting seasonal dynamics of dormancy, respiratory metabolism and cell cycle state in grapevine buds of a subtropical and Mediterranean climate
Authors: Velappan Yazhini , Considine John A. , Signorelli Santiago , Considine Michael J. 
Published: November 2022

AbstractGrapevine (Vitis vinifera L.) is the most widely cultivated fruit crop worldwide, contributing substantially to rural economies. The production cycle and productivity depend on seasonal cues and can range from a strongly deciduous habit in cool‐temperate climates to evergreen in subtropical and tropical climates. The influence of the different seasonal conditions on the dynamics of the perennating bud, including the degree of growth and metabolic quiescence, cell cycle status and internal tissue oxygen status between different climatic zones is largely unknown. This knowledge is important for adapting to changing climate conditions and for crop expansion to wider regions. This study investigated the growth and metabolic physiology of the perennating bud of commercially grown cv. Flame Seedless table grapes from Mediterranean and subtropical climate in Western Australia, from summer until late winter. Climate data were obtained, showing differences in minimum (night) temperature between the two climates, reflected by differences in calculated chilling units. Bud dormancy increased during autumn of both climates;however, the onset and depth of dormancy of buds from the subtropical region were attenuated relative to the Mediterranean condition. Stark contrasts were also observed in metabolism. The respiration of subtropical‐grown buds increased over fivefold during late autumn and winter, while that of Mediterranean‐grown buds increased less than twofold. This was also reflected in less desiccation of the subtropical‐grown buds, and an apparently greater degree of hypoxia within the bud during late winter, prior to bud burst. Collectively, these data show pronounced differences in growth and metabolic physiology of commercially grown table grapes, which provide a foundation for investigating the influence of differing climate and seasonality on the growth and productivity of table grapes and how these may be managed through breeding and agronomy.
An impact of heat and mass transpiration on magnetohydrodynamic viscoelastic fluid past a permeable stretching/shrinking sheet
Authors: Maranna T. , Sneha K. N. , Mahabaleshwar U. S. , Souayeh Basma 
Published: November 2022

AbstractThe present paper investigates analytically a continuous stream of viscoelastic fluid and magnetohydrodynamic flow of second‐grade fluids owing to protracted sheets in a permeable medium with the help of the Cattaneo–Christov pattern. This idea is a new generalization of the classical Fourier law. Also, in this analysis, heat as well as mass transfer in second‐grade fluid past wall suction/injection is assumed. A few similarity transformations are used to simplify the addressing of boundary layer expressions. An analytical solution is obtained by applying the Appell hypergeometric properties. Furthermore, our work also describes an effect of the relaxation time variable, elasticity number, and Prandtl number together with temperature fields. Also, we studied the newly introduced parameter, that is, the thermal radiation parameter by Cattaneo, over a Fourier heat flux pattern. In addition, some physical presentation of the measurements is illustrated in the graphs.
Cognitive impairment induced by sevoflurane anesthesia is mediated by the cholinergic system after gastrointestinal surgery in older patients: A randomized, controlled trial
Authors: Liu Xing‐Xing , Yang Qing‐Xu , Guo Yi , He Miao , Yu Zhen‐He , Tian Qi , Zhu Zhao‐Qiong 
Published: November 2022

AbstractDelayed neurocognitive recovery after surgery is associated with increased morbidity and mortality. However, its mechanism of action remains controversial and complex. A prospective, double‐blind, randomized controlled trial was performed at the Affiliated Hospital of Zunyi Medical University. Older patients (aged 65 years and older) who underwent gastrointestinal surgery were randomly divided into sevoflurane‐based or propofol‐based anesthesia groups. The Mini‐Mental State Examination was performed to evaluate cognitive function. Peripheral venous blood was collected to test the levels of choline acetyltransferase and acetylcholinesterase. A total of 75 patients were enrolled and 30 patients in each group completed the study. On Day 1 postoperation, patients in the sevoflurane group showed worse performance on the Mini‐Mental State Examination than patients in the propofol group. Lower blood choline acetyltransferase concentrations and higher acetylcholinesterase concentrations were observed in patients who had sevoflurane anesthesia than in patients who had propofol anesthesia 1 day postoperative. At 3 days postoperation, patients with sevoflurane‐ or propofol‐based general anesthesia did not differ regardless of Mini‐Mental State Examination score or choline acetyltransferase and acetylcholinesterase levels. Sevoflurane‐based anesthesia has short‐term delayed neurocognitive recovery in older surgical patients, which may be related to central cholinergic system degeneration.
A systematic review of longitudinal changes in school burnout among adolescents: Trajectories, predictors, and outcomes
Authors: Vansoeterstede Aline , Cappe Emilie , Lichtlé Jérôme , Boujut Emilie 
Published: November 2022

AbstractIntroductionSchool burnout (SBO) is a public health issue with possible long‐term consequences that occurs among developing adolescents. To design prevention policies, SBO's causes and consequences must be established. Moreover, a better understanding of its development might help identify key moments for interventions. Longitudinal studies can reveal whether SBO changes or remains stable over time and can track how its different dimensions develop. They can also clarify the distinction between the predictors and outcomes of SBO.MethodsThis systematic review follows PRISMA guidelines and aims to synthesize knowledge about the development of SBO and its predictors and outcomes. We queried databases for articles published between January 2002 and February 2021 that use a quantitative assessment of SBO and have longitudinal designs using students in secondary education.ResultsForty‐three articles representing 48 studies were included in this review. The most‐documented risk factors are female gender and high school academic track. Exhaustion is the first dimension of SBO to develop. The most‐documented risk factors are female gender and high school academic track. Relationships with parents serve as a protective factor. Outcomes of SBO include lower engagement at school, impaired identity development, and lower satisfaction with life. Complex relationships are found between SBO, problematic use of internet, problematic use of social media, and depressive symptoms. The three are likely to be caused by SBO but probably develop in vicious cycles.ConclusionMore research on the organizational factors influencing SBO in diverse educational systems is needed.
Beryllium bonding with noble gas atoms
Authors: Mazumder Lakhya Jyoti , Sharma Rohan , Yashmin Farnaz , Sharma Pankaz Kumar 
Published: November 2022

AbstractQuantum chemical calculations were carried out to investigate the nature of the bonding between a neutral Be3ring and noble gas atom. Electronic structure calculation for these complexes was carried out at different computational levels in association with natural bond orbital, quantum theory of atoms in molecules, electron localization function, symmetry adapted perturbation theory, and molecular electrostatic potential surface analysis of Be3complexes. The Be atoms in the Be3moiety are chemically bonded to one another, with the BeBe bond dissociation energy being ~125 kJ mol−1. The Be3ring interacts with the noble gases through non‐covalent interactions. The binding energies of the noble gas atoms with the Be3ring increases with increase in their atomic number. The non‐covalent interaction index, density overlap region indicator and independent gradient model analyses reveal the presence of non‐covalent inter‐fragment interactions in the complexes. Energy decomposition analysis reveals that dispersion plays the major role towards stabilizing these systems.