bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in microglia morphological profiles reflect divergent emotional temperaments: insights from a selective breeding model
Authors: Maras Pamela M. , Hebda-Bauer Elaine K. , Hagenauer Megan H. , Hilde Kathryn L. , Blandino Peter , Watson Stanley J. , Akil Huda 
Published: March 2022

AbstractMicroglia play critical roles in healthy brain development and function, as well as the neuropathology underlying a range of brain diseases. Despite evidence for a role of microglia in affective regulation and mood disorders, little is known regarding how variation in microglia status relates to individual differences in emotionality. Using a selective breeding model, we have generated rat lines with unique temperamental phenotypes that reflect broad emotional traits: bred low responder rats (bLRs) are novelty-averse and model a passive coping style, whereas bred high responder rats (bHRs) are highly exploratory and model an active coping style. To identify a functional role of microglia in these phenotypes, we administered minocycline, an antibiotic with potent microglia inhibiting properties and observed shifts in forced swim, sucrose preference, and social interaction behaviors in bLRs. Using detailed anatomical analyses, we compared hippocampal microglia profiles of bHRs and bLRs and found that although the lines had similar numbers of microglia, selective breeding was associated with a shift in the morphological features of these cells. Specifically, microglia from bLRs were characterized by a hyper-ramified morphology, with longer processes and more complicated branching patterns than microglia from bHRs. This morphology is thought to reflect an early stage of microglia activation and suggests that bLR microglia are in a reactive state even when animals are not overtly challenged. Taken together, our results provide novel evidence linking variation in inborn temperament with differences in the baseline status of microglia and implicate a role for microglia in shaping enduring emotional characteristics.
RETRACTED ARTICLE: Hepatic stellate cell mediates transcription of TNFSF14 in hepatocellular carcinoma cells via H2S/CSE-JNK/JunB signaling pathway
Authors: Ma Yanan , Wang Shanshan , Wu Yongle , Liu Bihan , Li Lei , Wang Wenjing , Weng Honglei , Ding Huiguo 
Published: March 2022

AbstractHepatic stellate cells (HSC) and hydrogen sulfide (H2S) both play important roles in the development of hepatocellar carcinoma (HCC). Whereas, in the microenvironment of HCC, whether HSC participate in regulating the biological process of HCC cells by releasing H2S remains elusive. In vitro, Flow cytometry (FCM), CCK-8, RNA-sequencing, Western blotting, RT-qPCR, immunofluorescence and ChIP assays were carried out in the HCC cells to investigate the effect of H2S on biological functions and JNK/JunB-TNFSF14 signaling pathway. Specimens from HCC patients were analyzed by RT-qPCR and Western blotting assays for evaluating the expression of TNFSF14 and CSE. Statistical analysis was used to analyze the correlation between TNFSF14 expression and clinical data of HCC patients. Based on the FCM and CCK-8 results, we found the LX-2 cells were able to induce HCC cells apoptosis through releasing H2S. RNA-sequencing, RT-qPCR, and Western blotting results showed that TNFSF14 gene was upregulated in both LX-2 and NaHS group. NaHS treated in HCC cells led to JNK/JunB signaling pathway activating and greater binding of p-JunB to its responsive elements on TNFSF14 promoter. Impairment of TNFSF14 induction alleviated LX-2 and NaHS induced apoptosis of HepG2 and PLC/PRF/5 cells. Furthermore, TNFSF14 expression in HCC tissues was lower than the adjacent tissue. HCC patients with low expression of TNFSF14 had higher malignant degree and poor prognosis. In summary, demonstration of the involvement of HSC-derived H2S in JNK/JunB mediated expression of TNFSF14 gene strongly indicates H2S palys an important role in the regulation of HCC apoptosis.
Dopamine D3 receptor signaling alleviates mouse rheumatoid arthritis by promoting Toll-like receptor 4 degradation in mast cells
Authors: Wang Biao , Li Xueyi , Li Ming , Geng Yan , Wang Na , Jin Yaofeng , Zhang Wen , Xu Ke , Wang Jing , Tao Li , Lai Simin , Wu Kunyi , Lei Jing , Wang Jing , Zhou Ting , Li Ke , Chen Yanjiong , Xue Li 
Published: March 2022

AbstractDopamine receptors are involved in several immunological diseases. We previously found that dopamine D3 receptor (D3R) on mast cells showed a high correlation with disease activity in patients with rheumatoid arthritis, but the mechanism remains largely elusive. In this study, a murine collagen-induced arthritis (CIA) model was employed in both DBA/1 mice and D3R knockout mice. Here, we revealed that D3R-deficient mice developed more severe arthritis than wild-type mice. D3R suppressed mast cell activation in vivo and in vitro via a Toll-like receptor 4 (TLR4)-dependent pathway. Importantly, D3R promoted LC3 conversion to accelerate ubiquitin-labeled TLR4 degradation. Mechanistically, D3R inhibited mTOR and AKT phosphorylation while enhancing AMPK phosphorylation in activated mast cells, which was followed by autophagy-dependent protein degradation of TLR4. In total, we found that D3R on mast cells alleviated inflammation in mouse rheumatoid arthritis through the mTOR/AKT/AMPK-LC3-ubiquitin-TLR4 signaling axis. These findings identify a protective function of D3R against excessive inflammation in mast cells, expanding significant insight into the pathogenesis of rheumatoid arthritis and providing a possible target for future treatment.
Projected climate-driven changes in pollen emission season length and magnitude over the continental United States
Authors: Zhang Yingxiao , Steiner Allison L. 
Published: March 2022

AbstractAtmospheric conditions affect the release of anemophilous pollen, and the timing and magnitude will be altered by climate change. As simulated with a pollen emission model and future climate data, warmer end-of-century temperatures (4–6 K) shift the start of spring emissions 10–40 days earlier and summer/fall weeds and grasses 5–15 days later and lengthen the season duration. Phenological shifts depend on the temperature response of individual taxa, with convergence in some regions and divergence in others. Temperature and precipitation alter daily pollen emission maxima by −35 to 40% and increase the annual total pollen emission by 16–40% due to changes in phenology and temperature-driven pollen production. Increasing atmospheric CO2 may increase pollen production, and doubling production in conjunction with climate increases end-of-century emissions up to 200%. Land cover change modifies the distribution of pollen emitters, yet the effects are relatively small (<10%) compared to climate or CO2. These simulations indicate that increasing pollen and longer seasons will increase the likelihood of seasonal allergies.
Stalagmite paleomagnetic record of a quiet mid-to-late Holocene field activity in central South America
Authors: Jaqueto Plinio , Trindade Ricardo I. F. , Terra-Nova Filipe , Feinberg Joshua M. , Novello Valdir F. , Stríkis Nicolás M. , Schroedl Peter , Azevedo Vitor , Strauss Beck E. , Cruz Francisco W. , Cheng Hai , Edwards R. Lawrence 
Published: March 2022

AbstractSpeleothems can provide high-quality continuous records of the direction and relative paleointensity of the geomagnetic field, combining high precision dating (with U-Th method) and rapid lock-in of their detrital magnetic particles during calcite precipitation. Paleomagnetic results for a mid-to-late Holocene stalagmite from Dona Benedita Cave in central Brazil encompass ~1900 years (3410 BP to 5310 BP, constrained by 12 U-Th ages) of paleomagnetic record from 58 samples (resolution of ~33 years). This dataset reveals angular variations of less than 0.06° yr−1 and a relatively steady paleointensity record (after calibration with geomagnetic field model) contrasting with the fast variations observed in younger speleothems from the same region under influence of the South Atlantic Anomaly. These results point to a quiescent period of the geomagnetic field during the mid-to-late Holocene in the area now comprised by the South Atlantic Anomaly, suggesting an intermittent or an absent behavior at the multi-millennial timescale.
Molecular basis for allosteric agonism and G protein subtype selectivity of galanin receptors
Authors: Duan Jia , Shen Dan-Dan , Zhao Tingting , Guo Shimeng , He Xinheng , Yin Wanchao , Xu Peiyu , Ji Yujie , Chen Li-Nan , Liu Jinyu , Zhang Huibing , Liu Qiufeng , Shi Yi , Cheng Xi , Jiang Hualiang , Eric Xu H. , Zhang Yan , Xie Xin , Jiang Yi 
Published: March 2022

AbstractPeptide hormones and neuropeptides are complex signaling molecules that predominately function through G protein-coupled receptors (GPCRs). Two unanswered questions remaining in the field of peptide-GPCR signaling systems pertain to the basis for the diverse binding modes of peptide ligands and the specificity of G protein coupling. Here, we report the structures of a neuropeptide, galanin, bound to its receptors, GAL1R and GAL2R, in complex with their primary G protein subtypes Gi and Gq, respectively. The structures reveal a unique binding pose of galanin, which almost ‘lays flat’ on the top of the receptor transmembrane domain pocket in an α-helical conformation, and acts as an ‘allosteric-like’ agonist via a distinct signal transduction cascade. The structures also uncover the important features of intracellular loop 2 (ICL2) that mediate specific interactions with Gq, thus determining the selective coupling of Gq to GAL2R. ICL2 replacement in Gi-coupled GAL1R, μOR, 5-HT1AR, and Gs-coupled β2AR and D1R with that of GAL2R promotes Gq coupling of these receptors, highlighting the dominant roles of ICL2 in Gq selectivity. Together our results provide insights into peptide ligand recognition and allosteric activation of galanin receptors and uncover a general structural element for Gq coupling selectivity.
Screening transition metal-based polar pentagonal monolayers with large piezoelectricity and shift current
Authors: Guo Yaguang , Zhou Jian , Xie Huanhuan , Chen Yanyan , Wang Qian 
Published: March 2022

AbstractTwo-dimensional (2D) materials entirely composed of pentagon motifs are of interest for their wide applications. Here, we demonstrate that in-plane polar symmetry can exist in ternary pentagonal monolayers, where the induced electric polarization is not associated with specific conditions, such as ferroelectric phase transition, strain gradient, and layer stacking, but is an intrinsic structural property coming from the orderly arranged polar bonds. Based on the high-throughput screening method and first-principles calculations, we find eight stable 2D polar transition metal compounds with a number of intriguing properties. In particular, their piezoelectric coefficients are three orders of magnitude larger than those of 2D elemental and binary pentagonal structures, and their bulk photovaltaic shift current can reach up to 300 μA V−2, superior to that of 2D conventional ferroelectric materials such as GeS. Our identified pentagonal monolayers not only expand the family of 2D pyroelectric materials, but also hold potential for energy conversions.
Storage of photonic time-bin qubits for up to 20 ms in a rare-earth doped crystal
Authors: Ortu Antonio , Holzäpfel Adrian , Etesse Jean , Afzelius Mikael 
Published: March 2022

AbstractLong-duration quantum memories for photonic qubits are essential components for achieving long-distance quantum networks and repeaters. The mapping of optical states onto coherent spin-waves in rare earth ensembles is a particularly promising approach to quantum storage. However, it remains challenging to achieve long-duration storage at the quantum level due to read-out noise caused by the required spin-wave manipulation. In this work, we apply dynamical decoupling techniques and a small magnetic field to achieve the storage of six temporal modes for 20, 50, and 100 ms in a 151Eu3+:Y2SiO5 crystal, based on an atomic frequency comb memory, where each temporal mode contains around one photon on average. The quantum coherence of the memory is verified by storing two time-bin qubits for 20 ms, with an average memory output fidelity of F = (85 ± 2)% for an average number of photons per qubit of μin = 0.92 ± 0.04. The qubit analysis is done at the read-out of the memory, using a type of composite adiabatic read-out pulse we developed.
Magneto-optical study of metamagnetic transitions in the antiferromagnetic phase of α-RuCl3
Authors: Wagner Julian , Sahasrabudhe Anuja , Versteeg Rolf B. , Wysocki Lena , Wang Zhe , Tsurkan Vladimir , Loidl Alois , Khomskii Daniel I. , Hedayat Hamoon , van Loosdrecht Paul H. M. 
Published: March 2022

Abstractα-RuCl3 is a promising candidate material to realize the so far elusive quantum spin liquid ground state. However, at low temperatures, the coexistence of different exchange interactions couple the effective pseudospins into an antiferromagnetically zigzag (ZZ) ordered state. The low-field evolution of spin structure is still a matter of debate and the magnetic anisotropy within the honeycomb planes is an open and challenging question. Here, we investigate the evolution of the ZZ order parameter by second-order magneto-optical effects, the magnetic linear dichroism and magnetic linear birefringence. Our results clarify the presence and nature of metamagnetic transitions in the ZZ phase of α-RuCl3. The experimental observations show the presence of initial magnetic domain repopulation followed by a spin-flop transition for small in-plane applied magnetic fields (≈1.6 T) along specific crystallographic directions. In addition, using a magneto-optical approach, we detected the recently reported emergence of a field-induced intermediate phase before suppressing the ZZ order. The results disclose the details of various angle-dependent in-plane metamagnetic transitions quantifying the bond-anisotropic interactions present in α-RuCl3.
Detection of subtle white matter lesions in MRI through texture feature extraction and boundary delineation using an embedded clustering strategy
Authors: Ong Kokhaur , Young David M. , Sulaiman Sarina , Shamsuddin Siti Mariyam , Mohd Zain Norzaini Rose , Hashim Hilwati , Yuen Kahhay , Sanders Stephan J. , Yu Weimiao , Hang Seepheng 
Published: March 2022

AbstractWhite matter lesions (WML) underlie multiple brain disorders, and automatic WML segmentation is crucial to evaluate the natural disease course and effectiveness of clinical interventions, including drug discovery. Although recent research has achieved tremendous progress in WML segmentation, accurate detection of subtle WML present early in the disease course remains particularly challenging. Here we propose an approach to automatic WML segmentation of mild WML loads using an intensity standardisation technique, gray level co-occurrence matrix (GLCM) embedded clustering technique, and random forest (RF) classifier to extract texture features and identify morphology specific to true WML. We precisely define their boundaries through a local outlier factor (LOF) algorithm that identifies edge pixels by local density deviation relative to its neighbors. The automated approach was validated on 32 human subjects, demonstrating strong agreement and correlation (excluding one outlier) with manual delineation by a neuroradiologist through Intra-Class Correlation (ICC = 0.881, 95% CI 0.769, 0.941) and Pearson correlation (r = 0.895, p-value < 0.001), respectively, and outperforming three leading algorithms (Trimmed Mean Outlier Detection, Lesion Prediction Algorithm, and SALEM-LS) in five of the six established key metrics defined in the MICCAI Grand Challenge. By facilitating more accurate segmentation of subtle WML, this approach may enable earlier diagnosis and intervention.
Loss of audiovisual facilitation with age occurs for vergence eye movements but not for saccades
Authors: Chavant Martin , Kapoula Zoï 
Published: March 2022

AbstractThough saccade and vergence eye movements are fundamental for everyday life, the way these movements change as we age has not been sufficiently studied. The present study examines the effect of age on vergence and saccade eye movement characteristics (latency, peak and average velocity, amplitude) and on audiovisual facilitation. We compare the results for horizontal saccades and vergence movements toward visual and audiovisual targets in a young group of 22 participants (mean age 25 ± 2.5) and an elderly group of 45 participants (mean age 65 + 6.9). The results show that, with increased age, latency of all eye movements increases, average velocity decreases, amplitude of vergence decreases, and audiovisual facilitation collapses for vergence eye movements in depth but is preserved for saccades. There is no effect on peak velocity, suggesting that, although the sensory and attentional mechanisms controlling the motor system does age, the motor system itself does not age. The loss of audiovisual facilitation along the depth axis can be attributed to a physiologic decrease in the capacity for sound localization in depth with age, while left/right sound localization coupled with saccades is preserved. The results bring new insight for the effects of aging on multisensory control and attention.
Engineered barriers regulate osteoblast cell migration in vertical direction
Authors: Chen X. , Xu Y. , Cheng Y. , Pang S. W. 
Published: March 2022

AbstractConsidering cell migration is essential for understanding physiological processes and diseases. The vertical migration of cells in three dimensions is vital, but most previous studies on cell migration have only focused on two-dimensional horizontal migration. In this paper, cell migration in the vertical direction was studied. Barriers with a height of 1, 5, 10, and 25 µm with grating and arrows in channels as guiding patterns were fabricated. The effects of barrier height and guiding patterns on the vertical migration of MC3T3 cells were explored. The study revealed that taller barriers hinder vertical migration of MC3T3 cells, whereas grating and arrows in channels promote it. The time-lapse and micrograph images showed that as the barrier height increased, the cell climbing angle along the barrier sidewall decreased, and the time taken to climb over the barrier increased. These results indicate that taller barriers increase the difficulty of vertical migration by MC3T3 cells. To promote the vertical migration of MC3T3 cells, 10 µm tall barriers with 18° and 40° sloped sidewalls were fabricated. For barriers with 18° sloped sidewalls, the probability for MC3T3 cells to climb up and down the 10 µm tall barriers was 40.6% and 20.3%, respectively; this is much higher than the migration probability over vertical barriers. This study shows topographic guidance on the vertical migration of MC3T3 cells and broadens the understanding of cell migration in the vertical direction.
Role of water in unexpectedly large changes in emission flux of volatile organic compounds in soils under dynamic temperature conditions
Authors: Parlin Asma Akter , Kondo Monami , Watanabe Noriaki , Nakamura Kengo , Wang Jiajie , Sakamoto Yasuhide , Komai Takeshi 
Published: March 2022

AbstractUnderstanding the diffusive transport behavior of volatile organic compounds (VOCs) in near-surface soils is important because soil VOC emissions affect atmospheric conditions and climate. Previous studies have suggested that temperature changes affect the transport behavior; however, the effect of these changes are poorly understood. Indeed, under dynamic temperature conditions, the change in VOC flux is much larger than that expected from the temperature dependency of the diffusion coefficient of VOCs in air. However, the mechanism is not well understood, although water in soil has been considered to play an important role. Here, we present the results of experiments for the upward vertical vapor-phase diffusive transport of two VOCs (benzene and tetrachloroethylene) in sandy soil under sinusoidal temperature variations of 20–30 °C, as well as its numerical representation. The results clarify that the unexpectedly large changes in emission flux can occur as a result of changes in the VOC concentration gradient due to VOC release (volatilization) from/trapping (dissolution) into water, and that such flux changes may occur in various environments. This study suggests the importance of a global evaluation of soil VOC emissions by continuous measurements in various soil environments and/or predictions through numerical simulations with thorough consideration of the role of water in dynamic soil environments.
Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection
Authors: Könemund Lea , Neumann Laurie , Hirschberg Felix , Biedendieck Rebekka , Jahn Dieter , Johannes Hans-Hermann , Kowalsky Wolfgang 
Published: March 2022

AbstractTraditional sensing technologies have drawbacks as they are time-consuming, cost-intensive, and do not attain the required accuracy and reproducibility. Therefore, new methods of measurements are necessary to improve the detection of bacteria. Well-established electrical measurement methods can connect high sensitive sensing systems with biological requirements. One approach is to functionalize an extended-gate field-effect transistor’s (EGFET) sensing area with modified porphyrins containing two different linkers. One linker connects the electrode surface with the porphyrin. The other linker bonds bacteria on the functional layer through a specific peptide chain. The negative charge on the surface of the cells regulates the surface potential which has an impact on the electrical behavior of the EGFET. The attendance of attached bacteria on the functionalized sensing area could successfully be detected.
Host inflammatory response and clinical parameters around implants in a rat model using systemic alendronate and zoledronate acid drug administrations
Authors: Kniha Kristian , Rink Lothar , Wolf Jana , Möhlhenrich Stephan Christian , Peters Florian , Heitzer Marius , Hölzle Frank , Modabber Ali 
Published: March 2022

AbstractImplant outcomes in comparison to a natural tooth in a rat model using systemic alendronate and zoledronate acid drug administrations were assessed. Fifty-four Sprague–Dawley rats were randomly allocated into two experimental groups (drug application of zoledronic acid; 0.04 mg/kg intravenously once a week and alendronic acid; 0.2 mg/kg subcutaneously five times a week) and one control group with 18 animals in each group. Drug delivery was conducted for a period of 4 months. After 4 weeks either a zirconia or a titanium implant was immediately inserted in the socket of the first molar of the upper jaw. In vivo investigations included host inflammatory parameters and the implant survival and success rates for up to 3 months. Material incompatibilities against titanium and zirconia nanoparticles were evaluated in vitro after stimulation of rat spleen cells. In vivo, IL-6 release around titanium implants demonstrated significantly higher values in the control group (p = 0.02) when compared to the zoledronic acid group. Around the natural tooth without drug administration, the control group showed higher IL-6 values compared with the alendronic acid group (p = 0.01). In vitro, only lipopolysaccharide and not the implant’s nanoparticles stimulated significant IL-6 and TNFα production. In terms of the primary aim of in vivo and in vitro IL-6 and TNFα measurements, no implant material was superior to the other. No significant in vitro stimulation of rat spleen cells was detected with respect to titanium oxide and zirconium oxide nanoparticles.