bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlates of treatment engagement and client outcomes: results of a randomised controlled trial of nabiximols for the treatment of cannabis use disorder
Authors: Mills Llewellyn , Dunlop Adrian , Montebello Mark , Copeland Jan , Bruno Raimondo , Jefferies Meryem , Mcgregor Iain , Lintzeris Nicholas 
Published: October 2022

AbstractIntroduction and aimsThere is increasing interest and evidence for the use of cannabinoid medications in the treatment of cannabis use disorder, but little examination of the correlates of successful treatment. This paper is a secondary analysis of a randomised placebo-controlled trial of nabiximols for the treatment of cannabis use disorder (CUD), aiming to identify which client and treatment characteristics impact treatment engagement and outcomes.MethodBayesian multiple regression models were used to examine the impact of age, gender, duration of regular cannabis use, daily quantity of cannabis, cannabis use problems, self-efficacy for quitting, sleep, mental health, pain measures, and treatment group upon treatment engagement (retention, medication dose, and counselling participation) and treatment outcomes (achieving end-of-study abstinence, and a 50% or greater reduction in cannabis use days) among the 128 clients participating in the 12-week trial.ResultsAmong the treatment factors, greater counselling attendance was associated with greater odds of abstinence and ≥ 50% reduction in cannabis use; nabiximols with greater odds of ≥ 50% reduction and attending counselling, and reduced hazard of treatment dropout; and higher dose with lower odds of ≥ 50% reduction. Among the client factors, longer duration of regular use was associated with higher odds of abstinence and 50% reduction, and lower hazard of treatment dropout; greater quantity of cannabis use with reduced hazard of dropout, greater odds of attending counselling, and higher average dose; greater pain at baseline with greater odds of ≥ 50% reduction and higher average dose; and more severe sleep issues with lower odds of ≥ 50% reduction. Males had lower odds of attending counselling.Discussions and conclusionsThese findings suggest that counselling combined with agonist pharmacotherapy may provide the optimal treatment for cannabis use disorder. Younger clients, male clients, and clients with sleep issues could benefit from extra support from treatment services to improve engagement and outcomes.Trial registrationAustralian New Zealand Clinical Trials Registry (ACTRN12616000103460)https://www.anzctr.org.au
Post-Acute COVID-19 syndrome (PACS) right atrioventricular and vena cava thrombus on top of a myxoma. A Case report
Authors: Alrifae Ghaliya Mohamed H , Almuquddami Amel Ahmed Said , Etaleb Khaled Masaud , Abdelhamid Mohamed Hadi Mohamed 
Published: October 2022

AbstractPost-Acute COVID-19 syndrome (PACS) is considered to be one of the least understood post-infectious syndromes. We report a case of a 21-year-old female who had a history of SARS-CoV-2 infection and presented with a right atrioventricular thrombus associated with pulmonary embolism and thrombocytopenia. At the time of admission, she was not vaccinated against SARS-CoV-2, and her serological tests for IgG and IgM antibodies against SARS-CoV-2 were positive. The size of the thrombus measured approximately 6 × 8 × 4 cm, which also led to tricuspid valve insufficiency due to mechanical dilatation of the valve’s ring. The right atrioventricular thrombus also extended up to the inferior vena cava, leading to mild congestive hepatomegaly. Moreover, during thrombectomy, the mass of the thrombus was attached to the interseptal right atrial wall. The histopathological assessment of the core mass revealed that it was a right atrial myxoma hidden inside that large thrombus. We suspect that the formation and propagation of the thrombus to that size occurs as a part of Post-Acute COVID-19 syndrome (PACS). This study reviews and discusses coronavirus disease 2019-relate to thrombus formation inside cardiac chambers in case of a cardiac tumor, like myxoma in the setting of post-acute phase COVID-19 syndrome.
GATA-type transcriptional factor SpGAT1 interacts with SpMIG1 and promotes lipid accumulation in the oleaginous yeast $$Saitozyma \ podzolica$$ zwy-2-3
Authors: Ran Yulu , Xu Hui , Yang Qingzhuoma , Xu Yi , Yang Huahao , Qiao Dairong , Cao Yi 
Published: October 2022

Abstract
Background
In oleaginous yeast, nitrogen limitation is a critical parameter for lipid synthesis. GATA-family transcriptional factor GAT1, a member of the target of rapamycin (TOR) pathway and nitrogen catabolite repression (NCR), regulates nitrogen uptake and utilization. Therefore, it is significant to study the SpGAT1 regulatory mechanism of lipid metabolism for conversion of biomass to microbial oil in $$Saitozyma \ podzolica$$

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Results
Compared with WT, $$\Delta gat1$$

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, and OE::gat1, the lipid yield of OE::gat1 increased markedly in the low carbon and nitrogen ratio (C/N ratio) mediums, while the lipid yield and residual sugar of $$\Delta gat1$$

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decreased in the high C/N ratio medium. According to yeast two-hybrid assays, SpGAT1 interacted with SpMIG1, and its deletion drastically lowered SpMIG1 expression on the high C/N ratio medium. MIG1 deletion has been found in earlier research to affect glucose metabolic capacity, resulting in a prolonged lag period. Therefore, we speculated that SpGAT1 influenced glucose consumption rate across SpMIG1. Based on yeast one-hybrid assays and qRT-PCR analyses, SpGAT1 regulated the glyoxylate cycle genes ICL1, ICL2, and pyruvate bypass pathway gene ACS, irrespective of the C/N ratio. SpGAT1 also could bind to the ACAT2 promoter in the low C/N medium and induce sterol ester (SE) accumulation.

Conclusion
Our findings indicated that SpGAT1 positively regulated lipid metabolism in S.podzolica zwy-2-3, but that its regulatory patterns varied depending on the C/N ratio. When the C/N ratio was high, SpGAT1 interacted with SpMIG1 to affect carbon absorption and utilization. SpGAT1 also stimulated lipid accumulation by regulating essential lipid anabolism genes. Our insights might spur more research into how nitrogen and carbon metabolism interact to regulate lipid metabolism.
MRI adipose tissue segmentation and quantification in R (RAdipoSeg)
Authors: Haugen Christine , Lysne Vegard , Haldorsen Ingfrid , Tjora Erling , Gudbrandsen Oddrun Anita , Sagen Jørn Vegard , Dankel Simon N. , Mellgren Gunnar 
Published: October 2022

Abstract
Background
Excess adipose tissue is associated with increased cardiovascular and metabolic risk, but the volume of visceral and subcutaneous adipose tissue poses different metabolic risks. MRI with fat suppression can be used to accurately quantify adipose depots. We have developed a new semi-automatic method, RAdipoSeg, for MRI adipose tissue segmentation and quantification in the free and open source statistical software R.

Methods
MRI images were obtained from wild-type mice on high- or low-fat diet, and from 20 human subjects without clinical signs of metabolic dysfunction. For each mouse and human subject, respectively, 10 images were segmented with RAdipoSeg and with the commercially available software SliceOmatic. Jaccard difference, relative volume difference and Spearman’s rank correlation coefficients were calculated for each group. Agreement between the two methods were analysed with Bland–Altman plots.

Results
RAdipoSeg performed similarly to the commercial software. The mean Jaccard differences were 10–29% and the relative volume differences were below ( ±) 20%. Spearman’s rank correlation coefficient gave p-values below 0.05 for both mouse and human images. The Bland–Altman plots indicated some systematic and proporitional bias, which can be countered by the flexible nature of the method.

Conclusion
RAdipoSeg is a reliable and low cost method for fat segmentation in studies of mice and humans.
Genomic characterization of peste des petits ruminants vaccine seed “45G37/35-k”, Russia
Authors: Kwiatek Olivier , Libeau Geneviève , Guendouz Samia , Corbanini Chloé , Gogin Andrey , Lunitsin Andrey , Sindryakova Irina , Koblasov Denis , Bataille Arnaud 
Published: October 2022

AbstractProduction of peste des petits ruminants (PPR) vaccines in Russia is based on two attenuated virus strains (“45G37/35-k” and “ARRIAH”) of common origin. Here, the identity of the strain PPRV/45G37/35-k was investigated using a full genome, Illumina deep sequencing approach. Phylogenomic analysis showed that PPRV/45G37/35-k belongs to the same lineage as the widely used PPRV vaccine strain Nigeria/75/1 (lineage II). However, 248 nucleotide differences separate the genomes of these vaccine strains, indicating that the PPRV vaccine strains produced in Russia are new strains not yet recognised by the World Organization for Animal Health (WOAH). Detailed information on the safety and efficacy of these vaccines should be provided to the WOAH before further national and international distribution.
Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations
Authors: Cortez Nunes Francisco , Letra Mateus Teresa , Taillieu Emily , Teixeira Sílvia , Carolino Nuno , Rema Alexandra , De Bruyckere Sofie , Gärtner Fátima , Haesebrouck Freddy , Amorim Irina 
Published: October 2022

AbstractBesides Helicobacter pylori, a Gram-negative bacterium that may cause gastric disorders in humans, non-Helicobacter pylori helicobacters (NHPH) may also colonize the stomach of humans and animals. In pigs, H. suis can induce gastritis and may play a role in gastric ulcer disease, possibly in association with Fusobacterium gastrosuis. In the present study, gastric samples from 71 slaughtered pigs and 14 hunted free range wild boars were tested for the presence of DNA of F. gastrosuis and gastric Helicobacter species associated with pigs, dogs cats and humans, using species-specific PCR assays, followed by sequencing of the amplicon. These gastric samples were also histopathologically evaluated. Almost all the pigs presented gastritis (95.8%). Helicobacter spp. were detected in 78.9% and F. gastrosuis in 35.2% of the animals. H. suis was the most frequently identified Helicobacter species (57.7% of the animals), followed by a H. pylori-like species (50.7%) and less often H. salomonis and H. felis (each in 2.8% of the animals). H. suis was most often detected in the glandular (distal) part of the stomach (pars oesophagea 9.9%, oxyntic mucosa 35.2%, antral mucosa 40.8%), while the H. pylori-like species was mainly found in the non-glandular (proximal) part of the stomach (pars oesophagea 39.4%, oxyntic mucosa 14.1%, antral mucosa 4.2%). The great majority of wild boars were also affected with gastritis (71.4%) and Helicobacter spp. and F. gastrosuis were detected in 64.3% and 42.9% of the animals, respectively. H. bizzozeronii and H. salomonis were the most frequently detected Helicobacter species, while a H. pylori-like species and H. suis were only occasionally identified. These findings suggest that these microorganisms can colonize the stomach of both porcine species and may be associated with gastric pathology. This should, however, be confirmed through bacterial isolation. This is the first description of the presence of F. gastrosuis DNA in the stomach of wild boars and a H. pylori-like species in the pars oesophagea of the porcine stomach.
Characterization of placental and decidual cell development in early pregnancy loss by single-cell RNA sequencing
Authors: Zheng Yuhua , Pan Jing , Xia Chenglai , Chen Haiying , Zhou Huadong , Ju Weina , Wegiel Jerzy , Myatt Leslie , Roberts James M. , Guo Xiaoling , Zhong Nanbert 
Published: October 2022

Abstract
Background
Early pregnancy loss (EPL) presents as sporadic or recurrent miscarriage during the first trimester. In addition to chromosomal defects, EPL may result from impairment of the placental-decidual interface at early gestational age due to gene-environmental interactions.

Methods
To better understand the pathogenesis associated with this impairment, cell development in chorionic villi and decidua of different forms of EPL (sporadic or recurrent) was investigated with single-cell RNA sequencing and compared to that of normal first-trimester tissue.

Results
Unique gene expression signatures were obtained for the different forms of EPL and for normal tissue and the composition of placental and decidual cell clusters in each form was established. In particular, the involvement of macrophages in the EPL phenotypes was identified revealing an immunoactive state.

Conclusion
Differential gene expression and unique marker genes among cell clusters from chorionic villi and decidua of miscarried and normal pregnancies, may lead to identification of biomarker for EPL.
A global super-grid: sociotechnical drivers and barriers
Authors: Hojckova Kristina , Ahlborg Helene , Sandén Björn A. 
Published: October 2022

Abstract
Background
One way to design an electricity system wholly based on renewables is referred to as the global Super-grid, a vision of a transmission network of unprecedented geographical scope that uses advanced technology to balance spatially and temporally varying supply and demand across the globe. While proponents, since the 1960s, have argued that a global Super-grid is technologically possible and socially desirable, and significant technical progress has been made since the 1990s, development is slow with new transmission lines being built predominantly with established technology and within the boundaries of single countries. The aim of this study is to explore sociotechnical drivers and barriers of global Super-grid development.

Results
A main driver is the century old ideas that larger grids are more efficient and contribute to cooperation and peace. Over the last decades, the level of technical knowledge and networks of proponent have grown. The Super-grid also benefits from the potential opportunity of building on existing grids. Barriers stem from the scale of investments needed to experiment, path dependences in established industry and competition from novel smaller scale solutions based on local production, energy storage and smart grid technology. Other barriers originate in the organisational and institutional complexities of international electricity trade, and in the lack of trust at local and global levels, which hinder the development of necessary coordination.

Conclusions
The analysis suggests that if the Super-grid is to become part of a future electricity system, the discourse needs to open up, move beyond simplistic ideas of efficiency and ‘technocratic internationalism’, and take into account a broader set of social benefits, risks and trade-offs.
Evaluation of quality and antimicrobial efficacy of locally manufactured alcohol-based hand sanitizers marketed in Addis Ababa, Ethiopia in the era of COVID-19
Authors: Selam Muluken Nigatu , Habte Bruck Messele , Marew Tesfa , Bitew Molalegne , Getachew Tigist , Getachew Solomon , Abate Atlaw , Mitiku Mequanint , Matsabisa Motlalepula , Birhanu Gebremariam 
Published: October 2022

Abstract
Background
The coronavirus disease 2019 (COVID-19) has been rapidly spreading across the globe since the World Health Organization (WHO) has declared the disease outbreak as a global pandemic on March 11, 2020. Hand hygiene, via either regular handwashing with soap and water or using hand sanitizers, is among the various measures that need to be followed to control the outbreak of the disease. Alcohol-based hand sanitizers (ABHS) are the “gold standard” for hand disinfection because of their broad antimicrobial spectrum of activity, easy availability, better safety profile, and general acceptability to users. This study aimed at evaluating the physicochemical quality and antimicrobial efficacy of the locally manufactured ABHS marketed in Addis Ababa, Ethiopia.

Methods
A cross-sectional survey was used to collect ABHS from Addis Ababa marketplaces. A total of 25 sample products were randomly selected from different categories of hand sanitizer manufacturers. The physicochemical evaluation of the products was carried out as per the United States Pharmacopoeia and WHO standards. Escherichia coli, Klebsiella spp., Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella spp., and Shigella spp clinical isolates were used for the antimicrobial efficacy test.

Results
The Fourier Transform Infrared result confirmed that all the test products met the identification test for ethanol. The majority (68%) of ABHS complied with the test for ethanol content (75–85% v/v). However, only 3 products fulfilled the hydrogen peroxide content (0.112–0.137% v/v). LPC307 showed the maximum zone of inhibition of 12 mm against Escherichia coli whereas MPC204 exhibited only 3 mm. LPC101 was found to be more sensitive to Shigella and Klebsiella Spp with minimum inhibitory concentration values of 20% and 10%, respectively. The sample product LPC101 showed a minimum bactericidal concentration of 20% against Escherichia coli, Pseudomonas aeruginosa, and Klebsiella spp.

Conclusion
One-third of the tested ABHS did not comply with the WHO ethanol content limit and the majority of the products failed to meet the label claim for hydrogen peroxide content. Besides, nearly all products proved that they have activity against all the tested pathogenic microorganisms at a minimum concentration from 10 to 80%; though, they did not show 99.9% bacteriostatic or bactericidal activities as claimed. The study findings suggested regular monitoring of the quality of marketed ABHS considering the current wide use of these products.
COVID-19 mortality in patients with immunodeficiency and its predictors: a systematic review
Authors: SeyedAlinaghi SeyedAhmad , Karimi Amirali , Barzegary Alireza , Mojdeganlou Hengameh , Vahedi Farzin , Mirghaderi Seyed Peyman , Shobeiri Parnian , Ramezani Maryam , Yousefi Konjdar Parisa , Mirzapour Pegah , Tantuoyir Marcarious M. , Mehraeen Esmaeil , Dadras Omid , Voltarelli Fabricio 
Published: October 2022

Abstract
Introduction
Patients with immunodeficiency are usually more prone to worse outcomes of infectious diseases. However, there are some disagreements in the context of COVID-19, for example, in patients with human immunodeficiency virus (HIV). Herein, we aimed to systematically review the risk and predictors of COVID-19 mortality in people with primary or secondary immunodeficiency.

Methods
PubMed, Scopus, Web of Science, and Science Direct were searched. We followed a two-step screening process to identify eligible results. We first reviewed the title and abstract of the records and the unqualified studies were removed. Then, their full texts were evaluated based on their coherence with the purpose and inclusion/exclusion criteria, and those eligible for qualitative synthesis were included.

Results
Twenty-two articles were included, which investigated a total of 109,326 with primary or secondary immunodeficiencies. Three studies investigated the pediatric and infant population, while other studies were conducted on the adult population. Overall, studies on both primary and secondary immunodeficiency conflicted as some reported higher and some mentioned lower mortality rates in patients with immunodeficiency.

Conclusions
Overall, there were two points of view in both types of immunodeficiencies. The first is the classical viewpoint that all immunodeficient patients are at a higher risk of infection leading to a higher mortality rate. The second types of studies found that immunodeficiency might play a less important or even an inverse role in mortality rates by lowering the severity of the inflammatory response. However, it is important to take note to comorbidities, such as DM, HTN, CAD, ESRD, history of lower respiratory infection, etc., and demographic factors, such as obesity and age > 70 years, as they appear to influence the mortality rate, especially in patients with secondary immunodeficiency.
Influence of social status, physical activity, and socio-demographics on willingness to pay for a basket of organic foods
Authors: Knaggs Julia , Pruitt J. Ross , Anderson Lindsay , Palma Marco 
Published: October 2022

AbstractConsumers are known to signal social status through their purchasing behaviors. As the food industry continually expands its use of strategic marketing to reach customers, understanding food’s connection to this kind of status signaling may open the door to explore new markets for farmers. This study explored the influence of social status, physical activity, and socio-demographics on an individual’s willingness to pay for a basket of high-quality organic foods. Over 3 days, participants had their physical activity measured by a pedometer, and they were randomly assigned to a social status condition and subsequently placed bids for the organic food basket using a second-price auction to measure their willingness to pay. High-status individuals were publicly recognized in order to test our hypothesis that individuals will not be motivated to pay more for an organic food basket than low-status counterparts when they have already received recognition for their high status. The results showed that on average non-students were willing to pay significantly more for an organic food basket than student counterparts. Hispanic and Asian shoppers were willing to pay more for an organic food basket than White counterparts. However, physical activity had no significant impact on willingness to pay. Ultimately, our hypothesis was confirmed that recognizing high-status individuals eliminated or reduced the need to showcase social status through higher bids for the organic food baskets.
MHY4571, a novel diarylcyclohexanone derivative, exerts anti-cancer activity by regulating the PKA-cAMP-response element-binding protein pathway in squamous cell lung cancer
Authors: Chung Jae Heun , Choi Ho Jung , Kang Yong Jung , Kim Yun Seong , Lee Sang-Yull , Kwon Ryuk Jun , Jeong Han-Sol , Park Su-Jung , Jeong Yeongmu , Kang Dongwan , Ko Jeongin , Noh SangGyun , Chung Hae Young , Moon Hyung Ryong , Yoon Seong Hoon 
Published: October 2022

Abstract
Background
The protein kinase A (PKA)/cAMP response element-binding protein (CREB) has been suggested to be related to the inhibition of the proliferation of non-small cell lung cancer (NSCLC) cells. This study aimed to investigate the efficacy of a novel diarylcyclohexanone derivative, MHY4571, in regulating the PKA-CREB pathway and to study its anti-tumor role in squamous NSCLC.

Methods
We designed MHY4571 as a novel PKA inhibitor with acceptable in silico ADME properties and tested it in vitro in lung cancer cell lines and in vivo in xenograft and orthotopic mouse models of squamous cell lung carcinoma.

Results
MHY4571 inhibited PKA activity (> 70% inhibition) and suppressed the expression of p-PKA and p-CREB dose-dependently. MHY4571 treatment reduced lung cancer cell viability and promoted caspase 3-dependent apoptotic cell death. Orally administered MHY4571 significantly suppressed lung tumor growth in xenograft and orthotopic mouse models. PKA catalytic subunit alpha-silencing by siRNA (siPKA) strongly attenuated CREB phosphorylation; siCREB did not alter PKA protein levels or its phosphorylation, suggesting that PKA is an upstream regulator of CREB activity. MHY4571 acted synergistically with cisplatin (on co-treatment) to induce apoptotic cell death in lung cancer cells.

Conclusions
Our results imply that MHY4571 may be a potential drug candidate for squamous cell lung cancer treatment.