bluuebunny/crossref_metadata_2025_split huggingface dataset:
An approach for elucidating dermal fibroblast dedifferentiation in amphibian limb regeneration
Authors: Satoh Akira , Kashimoto Rena , Ohashi Ayaka , Furukawa Saya , Yamamoto Sakiya , Inoue Takeshi , Hayashi Toshinori , Agata Kiyokazu 
Published: April 2022

AbstractUrodele amphibians,Pleurodeles waltlandAmbystoma mexicanum, have organ-level regeneration capability, such as limb regeneration. Multipotent cells are induced by an endogenous mechanism in amphibian limb regeneration. It is well known that dermal fibroblasts receive regenerative signals and turn into multipotent cells, called blastema cells. However, the induction mechanism of the blastema cells from matured dermal cells was unknown. We previously found that BMP2, FGF2, and FGF8 (B2FF) could play sufficient roles in blastema induction in urodele amphibians. Here, we show that B2FF treatment can induce dermis-derived cells that can participate in multiple cell lineage in limb regeneration. We first established a newt dermis-derived cell line and confirmed that B2FF treatment on the newt cells provided plasticity in cellular differentiation in limb regeneration. To clarify the factors that can provide the plasticity in differentiation, we performed the interspecies comparative analysis between newt cells and mouse cells and found thePde4bgene was upregulated by B2FF treatment only in the newt cells. Blocking PDE4B signaling by a chemical PDE4 inhibitor suppressed dermis-to-cartilage transformation and the mosaic knockout animals showed consistent results. Our results are a valuable insight into how dermal fibroblasts acquire multipotency during the early phase of limb regenerationviaan endogenous program in amphibian limb regeneration.
Perceived roles, benefits and barriers of virtual global health partnership initiatives: a cross-sectional exploratory study
Authors: Umphrey Lisa , Paasi George , Windsor William , Abongo Grace , Evert Jessica , Haq Heather , Keating Elizabeth M. , Lam Suet Kam , McHenry Megan S. , Ndila Carolyne , Nwobu Charles , Rule Amy , Tam Reena P. , Olson Daniel , Olupot-Olupot Peter 
Published: April 2022

Abstract
Background
Virtual global health partnership initiatives (VGHPIs) evolved rapidly during the COVID-19 pandemic to ensure partnership continuity. However the current landscape for VGHPI use and preference is unknown. This study aimed to increase understanding of GH partners’ perspectives on VGHPIs.

Methods
From 15 October to 30 November 2020, An online, international survey was conducted using snowball sampling to document pandemic-related changes in partnership activities, preferences for VGHPIs, and perceived acceptability and barriers. The survey underwent iterative development within a diverse author group, representing academic and clinical institutions, and the non-profit sector. Participants from their professional global health networks were invited, including focal points for global health partnerships while excluding trainees and respondents from the European Economic Area. Analysis stratified responses by country income classification and partnership type. Authors used descriptive statistics to characterize responses, defining statistical significance as α = 0.05.

Results
A total of 128 respondents described 219 partnerships. 152/219 (69%) partnerships were transnational, 157/219 (72%) were of > 5 years duration, and 127/219 (60%) included bidirectional site visits. High-income country (HIC) partners sent significantly more learners to low- to middle-income country (LMIC) partner sites (p < 0.01). Participants commented on pandemic-related disruptions affecting 217/219 (99%) partnerships; 195/217 (90%) were disruption to activities; 122/217 (56%) to communication; 73/217 (34%) to access to professional support; and 72/217 (33%) to funding. Respondents indicated that VGHPIs would be important to 206/219 (94%) of their partnerships moving forward. There were overall differences in resource availability, technological capacity, and VGHPI preferences between LMIC and HIC respondents, with a statistically significant difference in VGHPI acceptability (p < 0.001). There was no significant difference between groups regarding VGHPIs’ perceived barriers.

Conclusions
The pandemic disrupted essential partnership elements, compounding differences between LMIC and HIC partners in their resources and preferences for partnership activities. VGHPIs have the potential to bridge new and existing gaps and maximize gains, bi-directionality, and equity in partnerships during and after COVID-19.
Computer 3D modeling of radiofrequency ablation of atypical cartilaginous tumours in long bones using finite element methods and real patient anatomy
Authors: Rivas Loya Ricardo , Jutte Paul C. , Kwee Thomas C. , van Ooijen Peter M. A. 
Published: April 2022

Abstract
Background
Radiofrequency ablation (RFA) is a minimally invasive technique used for the treatment of neoplasms, with a growing interest in the treatment of bone tumours. However, the lack of data concerning the size of the resulting ablation zones in RFA of bone tumours makes prospective planning challenging, needed for safe and effective treatment.

Methods
Using retrospective computed tomography and magnetic resonance imaging data from patients treated with RFA of atypical cartilaginous tumours (ACTs), the bone, tumours, and final position of the RFA electrode were segmented from the medical images and used in finite element models to simulate RFA. Tissue parameters were optimised, and boundary conditions were defined to mimic the clinical scenario. The resulting ablation diameters from postoperative images were then measured and compared to the ones from the simulations, and the error between them was calculated.

Results
Seven cases had all the information required to create the finite element models. The resulting median error (in all three directions) was -1 mm, with interquartile ranges from -3 to 3 mm. The three-dimensional models showed that the thermal damage concentrates close to the cortical wall in the first minutes and then becomes more evenly distributed.

Conclusions
Computer simulations can predict the ablation diameters with acceptable accuracy and may thus be utilised for patient planning. This could allow interventional radiologists to accurately define the time, electrode length, and position required to treat ACTs with RFA and make adjustments as needed to guarantee total tumour destruction while sparing as much healthy tissue as possible.
Evolution of Indian cuisine: a socio-historical review
Authors: Antani Vishu , Mahapatra Santosh 
Published: April 2022

AbstractThis paper aims to present a socio-historical review of Indian cuisine and its evolution. Based on a thorough analysis of a wide range of documents, the investigation focuses on four eras, each having a significant and lasting effect on Indian cuisine. Parallels have been drawn to modern Indian cuisine wherever possible. This study follows the advances in Indian cuisine through the prehistoric era, into the Vedic era, where religious scriptures, especially the Hindu scriptures, affected what was cooked during the time, followed by the invasion of the subcontinent by the Mughals. Finally, the effects of European colonization on Indian cuisine are critically analysed and an attempt is made to examine threads, if any, connecting culinary preferences of people across these phases. The results of the analysis indicate the intriguing impact of intricate connections among several sociocultural factors on the evolution of Indian cuisine. At the end of the paper, a few areas are identified for future exploration.
The culinary culture, characteristics, and nutritional value of Su cuisine in China
Authors: Li Huilun , Ma Guansheng 
Published: April 2022

AbstractSu cuisine (Jiangsu cuisine) belongs to the Eight Great Cuisines in China. The origin of Su cuisine can be traced back to the Spring and Autumn period (771–476 B.C). The flourish of Su cuisine was the outcome of the economic boom in Tang (618–907) and Song (960–1279) Dynasties. The choice of ingredients is diverse since the region are rich in natural resources. After strict selection of ingredients and exquisite preparation of food, most dishes of Su cuisine have a fine presentation and a natural taste. Famous for the highly skilled processing technique, subtle flavor, and delicate appearance, Su cuisine is widely served on various occasions, including the state banquets of China. Currently, Cantonese and Sichuan cuisine are well accepted globally, while the international popularity and influence of Su cuisine remain low. Therefore, this article aims to introduce the culinary culture, characteristics, and traditional dishes of Su cuisine in detail. Moreover, a palatable taste is not equivalent to desirable health outcomes. The wide variety of vegetables, herbs, and high-quality animal proteins used in Su cuisine is allied with the recommended healthy eating behavior. On the other hand, some traditional Su cuisine dishes are rich in refined carbohydrates. Thus, we performed a literature review of the current scientific evidence regarding the health effect and nutritional value of Su cuisine. The related literature was searched in Chinese and English using the CNKI, WanFang Data, PubMed, Google Scholar, Web of Science, and Embase database.
Investigating expressed RNA variants that are related to disease severity in SARS-CoV-2-infected patients with mild-to-severe disease
Authors: Okendo Javan , Okanda David 
Published: April 2022

Abstract
Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be a significant public health challenge globally. SARS-CoV-2 is a novel virus, and the understanding of what constitutes expressed RNAseq variants in healthy, convalescent, severe, moderate, and those admitted to the intensive care unit (ICU) is yet to be presented. We characterize the different expressed RNAseq variants in healthy, severe, moderate, ICU, and convalescent individuals.

Materials and methods
The bulk RNA sequencing data with identifier PRJNA639275 were downloaded from Sequence Reads Archive (SRA). The individuals were divided into: (1) healthy, n = 34, moderate, n = 8, convalescent, n = 2, severe, n = 16, and ICU, n = 8. Fastqc version 0.11.9 and Cutadapt version 3.7 were used to assess the read quality and perform adapter trimming, respectively. STAR was used to align reads to the reference genome, and GATK best practice was followed to call variants using the rnavar pipeline, part of the nf-core pipelines.

Results
Our analysis demonstrated that different sets of unique RNAseq variants characterize convalescent, moderate, severe, and those admitted to the ICU. The data show that the individuals who recover from SARS-CoV-2 infection have the same set of expressed variants as the healthy controls. We showed that the healthy and SARS-CoV-2-infected individuals display different sets of expressed variants characteristic of the patient phenotype.

Conclusion
The individuals with severe, moderate, those admitted to the ICU, and convalescent display a unique set of variants. The findings in this study will inform the test kit development and SARS-CoV-2 patients classification to enhance the management and control of SARS-CoV-2 infection in our population.
Prognostic tools at hospital arrival in acute myocardial infarction: copeptin and hepatocyte growth factor
Authors: Pintado María-Consuelo , Maceda Lara , Trascasa María , Arribas Ignacio , De Pablo Raúl 
Published: April 2022

Abstract
Background
Prompt evaluation and treatment of acute coronary syndrome has demonstrated to reduce mortality. Although several biomarkers have been studied for risk stratification and prognostic purposes, none is recommended to guide treatment based on its prognostic value. Copeptin and hepatocyte growth factor have been associated with poor outcome in patients with acute myocardial infarction. The aim of this study is to evaluate the early prognostic value of measurements of copeptin and hepatocyte growth factor for hospital mortality risk and 1-year-follow-up mortality, in patients with acute myocardial infarction. In our retrospective observational study, we measured hepatocyte growth factor and copeptin in blood samples collected at hospital arrival in patients with acute myocardial infarction; and follow-up them until 1-year.

Results
84 patients with were included in the study, mainly male (65%) with a median age of 70.3 ± 13.56 years. Hospital mortality was 11.9%. Plasma levels of copeptin at hospital arrival were statistically significant higher in patients who died during hospital admission (145.60 pmol/L [52.21–588.50] vs. 24.79 pmol/L [10.90–84.82], p 0.01). However, we found no statistically significant association between plasma levels of hepatocyte growth factor and hospital mortality (381.05 pg/ml [189.95–736.65] vs. 355.24 pg/ml [175.55–521.76], p 0.73). 1-year follow-up mortality was 21.4%. Plasma levels of copeptin at hospital arrival were higher in those patients who died in the following year (112.28 pmol/L [25.10–418.27] vs. 23.82 pmol/L [10.96–77.30], p 0.02). In the case of HGF, we also find no association between hepatocyte growth factor plasma levels and 1 -year follow-up mortality (350.00 pg/ml [175.05–555.08] vs. 345.53 pg/ml [183.68–561.15], p 0.68).

Conclusions
In patients with acute myocardial infarction measurement of copeptin at hospital arrival could be a useful tool to assess the prognosis of these patients, since their elevation is associated with a higher hospital mortality and higher 1-year follow-up mortality. We have not found this association in the case of hepatocyte growth factor measurement.
Association between male sex hormones and tramadol abuse
Authors: Abdel-Moneim Wafaa Mohamed , Fawzy Mohammed , Mohammed Sarah Abdelsamee , Abdellah Nora Zeidan 
Published: April 2022

Abstract
Background
Tramadol dependence is prevalent across Egypt. The allegation that it can improve sexual function is the main reason for its popularity among young men. This study aims to determine the serum level of testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) in tramadol abusers.

Results
Serum testosterone (5.18 ± 1.95) and LH (7.79 ± 1.63) of abusers showed highly significant lower levels than control subjects, while FSH showed no significant difference between abusers and controls. There was no significant difference of hormonal levels in subjects according to the duration of abuse and dose of tramadol.

Conclusions
The results indicate that tramadol abuse causes a pronounced lowering of testosterone and LH levels that is not correlated to the duration of abuse and dose of tramadol.
Role of shear wave elastography in assessment of chronic allograft nephropathy
Authors: Barsoum Nadine R. , Elsisy Ali E. , Mohamed Moataz F. , Hassan Amr A. 
Published: April 2022

Abstract
Background
The principal cause of renal graft loss after the first year is chronic allograft nephropathy which is represented histologically by tubulo-interstitial fibrosis. Its early diagnosis and treatment are crucial to prevent late graft failure. Ultrasound is unequivocally the first-line imaging modality for the evaluation of renal transplants in the immediate postoperative period and for long-term follow-up. Ultrasound shear wave elastography is an imaging technique based on estimation of the elastic properties of tissues.
Elastography is performed in the same clinical setting with conventional B-mode ultrasonography. Tissue elasticity is displayed as an absolute number and color-coded real-time estimation. So, it can be used in screening and diagnosing chronic allograft nephropathy. However, the accurate diagnosis and prognosis of renal parenchymal complications still relies on tissue biopsy. Many studies have proved the high specificity of ultrasound elastography in decreasing the number of unnecessary biopsies.

Results
In our study, we included 36 patients with biopsy-proven chronic allograft nephropathy. All patients had a B-mode ultrasound examination and followed by ultrasound shear wave elastography in the same session. The results were compared to the histopathological results.
Time since transplantation was directly correlated with mean renal stiffness, revealing that with longer time of transplantation renal stiffness and interstitial fibrosis and tubular atrophy (IF/TA) percentage increased with r = 0.72, 0.90 and p value < 0.001.
Antero-posterior (AP) diameter of the renal graft was significantly correlated with mean renal stiffness as the larger the AP diameter, the higher the mean kidney stiffness with r = 0.47, 0.73 and p value 0.001.
Sensitivity analysis showed that US shear wave elastography through mean kidney stiffness can significantly predict moderate Banff score of renal fibrosis using cutoff value 28.67 kPa with sensitivity 87.5%, specificity 90%, AUC 0.91 and p value < 0.001.

Conclusion
Shear wave elastography (SWE) may be useful for the prediction of fibrosis in renal transplant patients, especially in the case of moderate Banff score, where the accuracy reached 87.5% using a cutoff value 28.67 kPa.
We conclude that US SWE can be of great help during the regular follow-up of renal transplant patients. It can act as a screening tool to identify patients with stiffness values that suggest moderate tubulo-interstitial fibrosis, so eventually helping in the early diagnosis, management and help in selecting patients who are candidate for biopsy and in avoiding the repeated unnecessary biopsies for others.
Modification of reconstruction threshold algorithm in two-dimensional LFMCW compressive radar
Authors: Ghanem Sameh 
Published: April 2022

AbstractNowadays, the Compressive Sensing theory has an important effect on many communication system’s performances, and one of these applications is the radar system. Applying CS in the radar such as Linear Frequency Modulation Continuous Wave (LFMCW) radar signals has many advantages but suffers from the processing time in the two-dimensional processing. The performance of the LFMCW radar signals is achieved by using both conventional Complex Approximate Message Passing (CAMP) and adaptive recovery algorithms using a suitable reduction factor in both range and Doppler directions. In this paper, a modification is made to the adaptive CAMP algorithm to enhance the radar detection performance compared to both the conventional and adaptive algorithms under the same conditions. One of the main problems in radar detection is off-pin targets, which can be overcome using a certain filter in both range and Doppler directions. A comparison is achieved among these algorithms concerning detection performance using Receiver Operating Characteristic curves and the resolution performance in both range and Doppler directions. During the system analysis, it was found that an enhancement was achieved using the modified algorithm in the radar performance without any degradation in both range and Doppler resolution compared with the other algorithms.
Longitudinal Changes in Physical Activity during Pregnancy: National Institute of Child Health and Human Development Fetal Growth Studies
Authors: MITRO SUSANNA D. , PEDDADA SHYAMAL , GLEASON JESSICA L. , HE DIAN , WHITCOMB BRIAN , RUSSO LINDSEY , GREWAL JAGTESHWAR , ZHANG CUILIN , YISAHAK SAMRAWIT F. , HINKLE STEFANIE N. , BUCK LOUIS GERMAINE M. , NEWMAN ROGER , GROBMAN WILLIAM , SCISCIONE ANTHONY C. , OWEN JOHN , RANZINI ANGELA , CRAIGO SABRINA , CHIEN EDWARD , SKUPSKI DANIEL , WING DEBORAH , GRANTZ KATHERINE L. 
Published: April 2022

ABSTRACT

Introduction
Exercise in pregnancy is associated with many perinatal benefits, but patterns of home, work, and commuting activity are not well described. We investigated longitudinal activity in singleton and twin pregnancy by activity domain and maternal characteristics.

Methods
In the National Institute of Child Health and Human Development Fetal Growth Studies cohorts, 2778 women with singleton and 169 women with twin gestations reported activity using the Pregnancy Physical Activity Questionnaire at up to six or seven study visits, respectively. Metabolic equivalent of task-hours per week (MET-h·wk−1) was calculated from reported activity. Baseline measurements (obtained between 10 and 13 wk) reflected past year activity. Linear mixed models estimated MET-h·wk−1 by domain (household/childcare, occupational, inactive, transportation, sports/exercise), self-reported race/ethnicity (non-Hispanic White, non-Hispanic Black, Hispanic, Asian/Pacific Islander), prepregnancy body mass index (<25, 25 to < 30, ≥30 kg·m−2), parity (0, ≥1), baseline activity (quartiles), and plurality (singleton, twin).

Results
Household/caregiving activity made up the largest fraction of reported MET-h·wk−1 at baseline (42%), followed by occupational activity (28%). Median summed activity declined 47%, from 297 to 157 MET-h·wk−1, between 10 and 40 wk, largely driven by changes in household/caregiving (44% decline), and occupational activity (63% decline). Sports/exercise activity declined 55% but constituted only 5% of reported MET-h·wk−1 at baseline. At baseline, non-Hispanic Black women reported significantly higher activity than non-Hispanic White or Hispanic women, but differences did not persist across pregnancy. Across gestation nulliparous women reported significantly lower activity than parous women. Women with singleton gestations reported significantly more activity than women with twins from weeks 26 to 38. Baseline activity level was strongly associated with later activity levels.

Conclusions
Measuring domains of activity beyond exercise, and collecting longitudinal measurements, is necessary to fully describe activity in diverse populations of pregnant women.
Brain Structure and Function Predict Adherence to an Exercise Intervention in Older Adults
Authors: MORRIS TIMOTHY P. , BURZYNSKA AGNIESZKA , VOSS MICHELLE , FANNING JASON , SALERNO ELIZABETH A. , PRAKASH RUCHIKA , GOTHE NEHA P. , WHITFIELD-GABRIELI SUSAN , HILLMAN CHARLES H. , MCAULEY EDWARD , KRAMER ARTHUR F. 
Published: April 2022

ABSTRACT

Introduction
Individual differences in brain structure and function in older adults are potential proxies of brain reserve or maintenance and may provide mechanistic predictions of adherence to exercise. We hypothesized that multimodal neuroimaging features would predict adherence to a 6-month randomized controlled trial of exercise in 131 older adults (age, 65.79 ± 4.65 yr, 63% female), alone and in combination with psychosocial, cognitive, and health measures.

Methods
Regularized elastic net regression within a nested cross-validation framework was applied to predict adherence to the intervention in three separate models (brain structure and function only; psychosocial, health, and demographic data only; and a multimodal model).

Results
Higher cortical thickness in somatosensory and inferior frontal regions and less surface area in primary visual and inferior frontal regions predicted adherence. Higher nodal functional connectivity (degree count) in default, frontoparietal, and attentional networks and less nodal strength in primary visual and temporoparietal networks predicted exercise adherence (r = 0.24, P = 0.004). Survey and clinical measures of gait and walking self-efficacy, biological sex, and perceived stress also predicted adherence (r = 0.17, P = 0.056); however, this prediction was not significant when tested against a null test statistic. A combined multimodal model achieved the highest predictive strength (r = 0.28, P = 0.001).

Conclusions
Our results suggest that there is a substantial utility of using brain-based measures in future research into precision and individualized exercise interventions older adults.