bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complementing the EGFR dynamic interactome using live-cell proximity labeling
Authors: van Gelder Charlotte A.G.H. , van Bergen Wouter , van Breugel Pieter C. , Altelaar Maarten 
Published: February 2022

AbstractThe epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase family (RTK) of transmembrane receptors, known to regulate many key cellular processes, including growth, proliferation, and differentiation. Its expression, activation, trafficking, and degradation have been extensively studied, as dysregulation of EGFR activation has been linked to a vast number of cancers. Activation of EGFR by different ligands results in distinct cellular responses, and the relative distribution of EGFR in different endosome pools in a process called endosomal sorting, leading to lysosomal degradation, or cell surface recycling, respectively, is considered a fundamental process in EGFR stimulation outcome. The EGFR interactome is therefore an essential element in the study of RTK functional selectivity. Here, we aimed to complement the existing EGFR interactome with spatio-temporal information on EGFR, its interactors, and phosphorylation state. We identified and quantified EGFR stable and transient interactions at different time points after stimulation using an EGFR-APEX2 fusion construct expressed in HEK293T cells and were able to use bystander proteins to map EGFR subcellular location at each time point. Utilizing the fast and concise biotinylation of proximity proteins by APEX2, we were able to detect slight differences in early signaling kinetics between TGF-α and EGF, thereby increasing our knowledge on RTK signaling and differential trafficking.
Determining the targeting specificity of the selective peroxisomal targeting factor Pex9
Authors: Yifrach Eden , Rudowitz Markus , Cruz-Zaragoza Luis Daniel , Tirosh Asa , Gazi Zohar , Peleg Yoav , Kunze Markus , Eisenstein Miriam , Schliebs Wolfgang , Schuldiner Maya , Erdmann Ralf , Zalckvar Einat 
Published: February 2022

AbstractTargeting proteins to their correct cellular location is a fundamental process that allows them to carry out their cellular functions. Peroxisomes utilize two paralog targeting factors, Pex5 and Pex9, for proteins with a Peroxisomal Targeting Signal 1 (PTS1). However, in spite of their similarity, Pex9 targets only a subset of Pex5 cargo proteins. Here, we studied the properties that facilitate the targeting specificity of Pex9, both by unbiased screens and by site-directed mutagenesis of the PTS1 motifs of either binders or non-binders. We find that the binding specificity of Pex9 is largely determined by the hydrophobic nature of the amino acid preceding the PTS1 tripeptide of its cargos. This is in line with structural modeling of the PTS1-binding cavity of the two factors, showing that while Pex5 has large negative electrostatic patches at the area surrounding the PTS1 binding cavity, Pex9 is mostly hydrophobic. Our work outlines the mechanism by which targeting specificity is achieved, enabling dynamic rewiring of the peroxisomal proteome in changing metabolic needs.
Fish oil Nano-emulsion Kills Macrophage: Ferroptosis and Autophagy Triggered by Catalase-catalysed Superoxide Eruption
Authors: Gao Guanzhen , Zhou Jingru , Wang Huiqin , Ke Lijing , Zhou Jianwu , Ding Yanan , Ding Wei , Zhang Suyun , Rao Pingfan 
Published: February 2022

ABSTRACTFish oil is increasingly utilized in the form of nano-emulsion as nutrient and function fortifier. The nano-emulsion’s high polyunsaturated fatty acids content and electron donors at oil/water interface could be a potential site of redox reaction, if a previously unrecognised trigger was provided. Here we report that a vigorous superoxide production occurred in fish oil nano-emulsion in the presence of mammalian catalase in both acellular and cellular systems. Furthermore, the resulting superoxide increased cytosolic ROS and membrane lipid peroxidation of murine macrophage, and eventually caused fatal oxidative damage, which involves autophagy and ferroptosis but not apoptosis. The cell death was significantly inhibited by a catalase-specific inhibitor. The ferroptosis was independent of protease Caspase-3 activation or glutathione peroxidase suppression. Our findings discover a hidden risk factor of the widely acclaimed fish oil emulsion and suggest a novel mechanism of cellular damage by dietary lipids on mucosal layer of the alimentary tract.
A new fold in TANGO1 evolved from SH3 domains for the export of bulky cargos
Authors: Arnolds Oliver , Stoll Raphael 
Published: February 2022

AbstractBulky cargos like procollagens, apolipoproteins, and mucins exceed the size of conventional COPII vesicles. During evolution a process emerged in metazoans, predominantly governed by the TANGO1 protein family, that organizes cargo at the exit sites of the endoplasmic reticulum and facilitates export by the formation of tunnel-like connections between the ER and Golgi. Cargorecognition appears to be mediated by an SH3-like domain, however, just how the vastly different cargos are recognized remains elusive. Based on structural and dynamic data as well as interaction studies from NMR spectroscopy presented here, we show that the luminal cargo-recognition domain of TANGO1 adopts a new functional fold for which we suggest the term MOTH (MIA, Otoraplin, TALI/TANGO1 homology) domain. Also, differences in the structural properties within the domain family suggest fundamentally different mechanisms of cargo-recognition in vertebrates and invertebrates. Similarly, in vertebrates, it is proposed that cargo-specificity is mediated by structural differences within the vertebrate TANGO1 and TALI MOTH domains themselves.
Functional crosstalk between the carrier translocase machinery and YME1 complex maintains mitochondrial proteostasis and integrity
Authors: Kumar Abhishek , D’Silva Patrick 
Published: February 2022

AbstractThe TIM22 pathway cargos are essential for sustaining mitochondrial proteostasis as an excess of these proteins leads to proteostatic stress and cell death. Yme1 is an inner membrane metalloprotease that regulates proteostasis with its chaperone-like and proteolytic activities. Although the mitochondrial translocase and protease machinery are critical for organelle health, the functional link between these complexes remains unexplored. The present study unravels a novel genetic connection between the TIM22 complex and YME1 machinery in maintaining mitochondrial proteostasis and quality control. Our genetic analyses indicate that impairment in the TIM22 complex rescues the respiratory growth defects of cells without Yme1. We further demonstrate that Yme1 is essential for the stability of the TIM22 complex and regulating the proteostasis of the TIM22 pathway substrates. Moreover, impairment in the TIM22 complex suppressed the mitochondrial structural and functional defects of Yme1 devoid cells. Notably, the functional dependence between the TIM22 and YME1 complexes remains functionally conserved from yeast to humans. Our findings suggest that excessive levels of the TIM22 pathway substrates could be one of the reasons for the respiratory growth defects of cells lacking Yme1 and compromising the TIM22 complex compensate for the imbalance in mitochondrial proteostasis caused by loss of Yme1.
Recruitment of transcriptional effectors by Cas9 creates cis regulatory elements and demonstrates distance-dependent transcriptional regulation
Authors: Boulos Jubran , Ehrlich Itai , Avidan Nili , Shkedi Omer , Haimovich-Caspi Lilac , Kaplan Noam , Kehat Izhak 
Published: February 2022

AbstractIt is essential to regulate the expression of genes, such as those encoding the proteins of the cardiac sarcomere. This regulation is often mediated by cis regulatory elements termed enhancers and repressors that recruit transcription factors to gene-distal sites. However, the relationship between transcription factors recruitment to gene-distant sites and the regulation of gene expression is not fully understood. Specifically, it is unclear if such recruitment to any genomic site is sufficient to form an enhancer or repressor at the site, and what is the relationship between the cis regulatory element’s position and its ability to control the transcription of distant genes. Using dead Cas9 to recruit either viral or endogenous transcription factor activation domains, we demonstrate that targeting ‘naïve’ genomic sites lacking open chromatin or active enhancer marks is sufficient to alter the chromatin signature of the target site, the distant gene promoter, and significantly induce the distant gene expression, even across chromatin insulating loci. The magnitude of induction is affected by the distance between the activation site and the cognate gene in a non-linear manner. Dead Cas9 mediated recruitment of repression domains behave similarly to activation in that targeting of non-regulatory regions could repress gene expression with a nonlinear distance dependence and across chromatin insulating loci. These findings expand the models of enhancer generation and function by showing that an arbitrary genomic site can become a regulatory element and interact epigenetically and transcriptionally with a distant promoter. They also provide new fundamental insights into the rules governing gene expression.
Application of theory of planned behavior in identifying factors affecting online health information seeking intention and behavior of women
Authors: Shamlou Zahra , Saberi Mohammad Karim , Amiri Mohammad Reza 
Published: February 2022

PurposeToday, the Internet has become an attractive source for obtaining health information. Since women play an important role in maintaining the health of the family and community and since they are active seekers of health information through the Internet, it is essential to examine their online health information seeking behavior. Therefore, the main purpose of this article is to identify the factors affecting the online health information seeking intention and behavior of women.Design/methodology/approachThe theoretical framework of this quantitative study was based on the theory of planned behavior. A total of five hypothesized relationships were formulated to develop a conceptual model. The study approach was quantitative. Using simple random sampling, 400 women referred to specialized and sub-specialized clinics of Hamadan University of Medical Sciences, Iran participated in the study and responded to the survey. Descriptive and inferential data analysis was performed using SPSS 21 and Smart PLS 3 software.FindingsThe results showed that the intention to find health information online is a function of psychological variables. These variables include attitude, subjective norms and perceived behavioral control. Findings showed that attitude is the most important factor affecting online health information seeking intention. It was also found that online health information seeking intention has a positive and significant effect on women's information seeking behavior.Originality/valueThis study is the first attempt to use an important and universally accepted theory to identify the factors affecting the online health information seeking behavior of women referring to specialized and sub-specialized clinics and provides important practical concepts for health officials and managers to improve and develop online health information seeking behavior.
HRM practices, impersonal trust and service oriented OCB: an empirical evidence from Bangladesh
Authors: Sultana Sarmin , Johari Husna 
Published: February 2022

PurposeThe preliminary purpose of the study is to explore the relationship between Human Resource Management (HRM) practices and service oriented organizational citizenship behavior (SO-OCB). The current study’s focus is on three HRM practices namely training, fair reward and promotion opportunity. Second, the study examines the mediating role of impersonal trust on the aforementioned relationship through the use of social exchange theory.Design/methodology/approachData has been collected through purposive sampling technique from service employees of 39 private commercial banks of Bangladesh and was analyzed by using structural equation modeling-partial least square method.FindingsFindings of the study comply with the theory of social exchange. All three HRM practices significantly predict SO-OCB. Additionally, impersonal trust positively mediates the relationship between HRM practices and SO-OCB.Originality/valueAs private commercial banks are growing rapidly in Bangladesh, this sector is facing enormous competition which makes frontline employees SO-OCB a competitive advantage. Due to the limited researches in the field of SO-OCB, this study provides a new avenue of knowledge for the researchers and practitioners on how to develop such behavior in a service setting. Further, no prior study tests the mediation of impersonal trust. Earlier, most of the studies focus on interpersonal trust. The current study contributes to the literature by offering a comprehensive framework that explains the mediation effect of impersonal trust on the association between HRM practices and SO-OCB.
Implications of neuroarchitecture for the experience of the built environment: a scoping review
Authors: Lee Sanghee , Shin Wooree , Park Eun Joo 
Published: February 2022

PurposeNeuroarchitecture is a new interdisciplinary research field combining neuroscience and architecture that has developed and expanded since 2000. Neuroarchitecture originated from the divergence of previous multidisciplinary studies on the relationship between humans and the environment. However, scoping reviews of neuroarchitecture in relation to the experience of the built environment are lacking. Thus, this study aimed to provide the background and research trends of neuroarchitecture to contribute to discussions on the built environment.Design/methodology/approachA general form of scoping review was adopted, following the PRISMA-ScR checklist. For this scoping review emphasizing the embodied implication of neuroarchitecture for the built environment, an evaluation framework was developed consisting of four categories: health, performance, aesthetics and emotion.FindingsThis study explores objective techniques, including electroencephalography and functional magnetic resonance imaging, electrocardiogram, electrodermal activity and saliva cortisol, to measure neurophysiological impacts, adopting real, virtual and images of environmental settings. An in-depth review of 25 selected papers revealed the existing empirical research on neuroarchitecture using human physiological measurement tools and representational environment settings to examine the impact of human–environment relationships.Originality/valueA meta-analysis of theoretical and intervention studies on neuroarchitecture that investigates the multisensory characteristics of the environment is lacking. In addition, the development and application of wearable tools to meet the needs of real environment settings can improve the effectiveness of neurophysiological measurement tools.
Impact of supply chain performance on organizational performance mediated by customer satisfaction: a study of dairy industry
Authors: Maaz Mohd Abdul Muqeet , Ahmad Rais 
Published: February 2022

PurposeThe aim of this study is to empirically test the relationship between supply chain performance and organizational performance mediated by customer satisfaction.Design/methodology/approachRespondents of this study include all the supply chain stakeholders, managers working in the dairy industry, bulk milk chillers/coolers (BMCs), farmer producers, wholesalers, transporters, retailers and consumers. The final sample has 67 firms and their forward and backward linkages, taking the total sample size to 1,139. Mediation analysis was performed using SPSS AMOS v26 to analyze the impact of supply chain performance on organizational performance mediated by customer satisfaction.FindingsThe findings of this study reveal that supply chain performance positively impacts organizational performance, and customer satisfaction partially mediates the relationship between them.Research limitations/implicationsThis study provides an opportunity for future researchers to test this model in different locations and different industries, incorporating other mediating variables, such as risk, coordination, supply chain orientation etc., as antecedents and operational, economic and marketing performance as consequences.Practical implicationsThe study suggests that enhanced organizational performance requires supply chain management to be treated as a strategic function. Managers working at the processing/manufacturing level must focus on improving supply chain performance indicators through collaboration with supply chain stakeholders.Originality/valueThe present study adds to the existing literature by adopting a new supply chain performance measurement approach. The current literature has focused primarily on supply chain practices to study their impact on customer satisfaction and organizational performance. In contrast, the present study demonstrates how improvement in supply chain performance can enhance organizational performance through customer satisfaction.
Broad vs narrow brand positioning: effects on competitive brand performance
Authors: Olsen Lars Erling , Meling Samuelsen Bendik , Pappas Ioannis , Warlop Luk 
Published: February 2022

Purpose
Brand managers can choose among two fundamentally different brand positioning strategies. One is a broad brand strategy, focusing on many favorable brand associations. The other is a narrow brand strategy, focusing on just a few and thus more mentally accessible associations. Building on associative memory theory, this paper aims to examine which of these brand positioning strategies performs better under dynamic market conditions.

Design/methodology/approach
Three experiments test the effect of brand positioning strategy on memory accessibility and competitive brand performance. Study 1 tests how brand strategy (broad vs narrow) affects defensive brand performance. Study 2 tests how broad vs narrow brands perform differently in a brand extension scenario (offensive brand performance). Study 3 uses real brands and situation-based attributes as stimuli in a defensive scenario.

Findings
The results show that a narrow brand positioning strategy leads to a competitive advantage. Narrow brands with fewer and more accessible associations resist new competitors more easily and have higher brand extension acceptance than do broad brands.

Research limitations/implications
The study shows how to use accessibility as evidence of associative strength and test how accessibility influences competitive brand performance in a controlled experimental context.

Practical implications
Brand managers would benefit from a narrow brand positioning strategy in accordance with the unique selling proposition (USP) school of thought used by many marketing practitioners.

Originality/value
The paper demonstrates that narrow brand positioning performs better than broad brand positioning in dynamic markets, and to the knowledge is the first to do so.
MoDeT: a low-cost obstacle tracker for self-driving mobile robot navigation using 2D-laser scan
Authors: Nguyen Toan Van , Do Minh Hoang , Jo Jaewon 
Published: February 2022

Purpose
Collision avoidance is considered as a crucial issue in mobile robotic navigation to guarantee the safety of robots as well as working surroundings, especially for humans. Therefore, the position and velocity of obstacles appearing in the working space of the self-driving mobile robot should be observed to help the robot predict the collision and choose traversable directions. This paper aims to propose a new approach for obstacle tracking, dubbed MoDeT.

Design/methodology/approach
First, all long lines, such as walls, are extracted from the 2D-laser scan and considered as static obstacles (or mapped obstacles). Second, a density-based procedure is implemented to cluster nonwall obstacles. These clusters are then geometrically fitted as ellipses. Finally, the combination of Kalman filter and global nearest-neighbor (GNN) method is used to track obstacles’ position and velocity.

Findings
The proposed method (MoDeT) is experimentally verified by using an autonomous mobile robot (AMR) named AMR SR300. The MoDeT is found to provide better performance in comparison with previous methods for self-driving mobile robots.

Research limitations/implications
The robot can only see a part of the object, depending on the light detection and ranging scan view. As a consequence, geometrical features of the obstacle are sometimes changed, especially when the robot is moving fast.

Practical implications
This proposed method is to serve the navigation and path planning for the AMR.

Originality/value
(a) Proposing an extended weighted line extractor, (b) proposing a density-based obstacle detection and (c) implementing a combination of methods [in (a) and (b) constant acceleration Kalman and GNN] to obtain obstacles’ properties.
Understanding online information disclosure: examination of data breach victimization experience effect
Authors: Ayaburi Emmanuel W. 
Published: February 2022

PurposeThe study aims to empirically understand individuals' tendency to disclose private information online following different forms of data breach (i.e. reversible and irreversible victimization).Design/methodology/approachSurvey methodology is applied to measure the perception of victims of data breaches on key indicators of information disclosure.FindingsAnalysis of responses from 309 victims of data breaches show that while victims' irreversible data breach victimization experience influences both dimensions of privacy concerns, reversible data breach victimization experiences influenced only peer privacy concerns (PPCs). Furthermore, only institutional privacy concerns impacted online disclosure and fully mediate the relationship between victimization experience and online disclosure.Research limitations/implicationsThe findings contribute to the privacy literature by expanding the dimension of victimization and considering their differential effect on privacy concerns. Additionally, the study uncovers the efficacy of privacy dimension on privacy recalibration following a data breach announcement.Practical implicationsFor practice, the results provide insights for managers on how to manage customer restitution after a data breach. Management of the process of privacy recalibration should not be homogenous but be based on degree of consequence.Social implicationsThis research provides deeper understanding of how the ascendancy of privacy breaches affect privacy management. The findings illuminate why the increasing trend in online activities is observed.Originality/valueThe study is the first to identify two dimensions of data breach victimization experience based on the breach level index (BLI). The two dimensions of victimization (i.e. reversible and irreversible privacy victimizations) were used to understand individuals' tendency to disclose private information online.