bluuebunny/crossref_metadata_2025_split huggingface dataset:
The feasibility and reliability of measuring forearm muscle thickness by ultrasound in a geriatric inpatient setting: a cross-sectional pilot study
Authors: Meza-Valderrama Delky , Sánchez- Rodríguez Dolores , Perkisas Stany , Duran Xavi , Bastijns Sophie , Dávalos-Yerovi Vanesa , Da Costa Elizabeth , Marco Ester 
Published: February 2022

Abstract
Background
Given the potential benefits of introducing ultrasound in the clinical assessment of muscle disorders, this study aimed to assess the feasibility and reliability of measuring forearm muscle thickness by ultrasound in a geriatric clinical setting.

Methods
Cross-sectional pilot study in 25 participants (12 patients aged ≥ 70 years in an acute geriatric ward and 13 healthy volunteers aged 25–50 years), assessed by three raters. Muscle thickness measurement was estimated as the distance between the subcutaneous adipose tissue-muscle interface and muscle-bone interface of the radius at 30% proximal of the distance between the styloid process and distal insertion of the biceps brachii muscle of the dominant forearm. Examinations were repeated three times by each rater and intra- and inter-rater reliability was calculated. Feasibility analysis included consideration of technological, economic, legal, operational, and scheduling (TELOS) components.

Results
Mean muscle-thickness measurement difference between groups was 4.4 mm (95% confidence interval [CI] 2.4 mm to 6.3 mm], p < 0.001). Intra-rater reliability of muscle-thickness assessment was excellent, with intraclass correlation coefficient (ICC) of 0.947 (95%CI 0.902 to 0.974), 0.969 (95%CI 0.942 to 0.985), and 0.950 (95%CI 0.907 to 0.975) for observer A, B, and C, respectively. Inter-rater comparison showed good agreement (ICC of 0.873 [95%CI 0.73 to 0.94]). Four of the 17 TELOS components considered led to specific recommendations to improve the procedure’s feasibility in clinical practice.

Conclusion
Our findings suggest that US is a feasible tool to assess the thickness of the forearm muscles with good inter-rater and excellent intra-rater reliability in a sample of hospitalized geriatric patients, making it a promising option for use in clinical practice.
Two-year immune effect differences between the 0–1–2-month and 0–1–6-month HBV vaccination schedule in adults
Authors: Wang Juan , Liu Chang-Hai , Ma Yuanji , Zhu Xia , Luo Liru , Ji Yulin , Tang Hong 
Published: February 2022

Abstract
Background
The short-term 0–1–2-month hepatitis B virus (HBV) vaccination schedule was previously implemented in the adult population; however, its long-term immune effect remains unclear. The present study aimed to investigate (1) the 2-month and 2-year immune effects of HBV vaccination and (2) the compliance rate between the 0–1–2-month and 0–1–6-month vaccination schedules in adults.

Method
A total of 1281 subjects tested for hepatitis B surface antigen HBsAg(−) and hepatitis B surface antibody (anti-HBs)(−) were recruited. Participants from two distant counties were inoculated with the hepatitis B yeast vaccine at 10 µg per dose, with vaccination schedules of 0, 1, and 2 months (n = 606) and 0, 1, and 6 months (n = 675); sequential follow-up was performed at 2 months and 2 years after the 3rd injection.

Results
There were no significant differences in the anti-HBs seroconversion rates between the those in the 0–1–2-month and 0–1–6-month vaccination schedule groups at 2 months (91.96% vs. 89.42%, p = 0.229) and 2 years (81.06% vs. 77.14%, p = 0.217). The quantitative anti-HBs level in those in the 0–1–2-month vaccination schedule group was not different from that in those in the 0–1–6-month vaccination schedule group at 2 months (anti-HBs1) (342.12 ± 378.42 mIU/ml vs. 392.38 ± 391.96 mIU/ml, p = 0.062), but it was higher at 2 years (anti-HBs2) (198.37 ± 286.44 mIU/ml vs. 155.65 ± 271.73 mIU/ml, p = 0.048). According to the subgroup analysis, the 0–1–2-month vaccination schedule induced better maintenance (p = 0.041) and longer reinforcement (p = 0.019) than the 0–1–6 vaccination schedule. The 0–1–2-month vaccination schedule group also had a higher 3rd injection completion rate (89.49% vs. 84.49%, p = 0.010).

Conclusion
The 0–1–2-month vaccination schedule was associated with a similar short-term immune effect and might induce better long-term immune memory and a higher completion rate in the adult population.
Trial registration None
Audit identified modifiable factors in Hospital Care of Newborns in low-middle income countries: a scoping review
Authors: Ogola Muthoni , Njuguna Emily Mbaire , Aluvaala Jalemba , English Mike , Irimu Grace 
Published: February 2022

Abstract
Background
Audit of facility-based care provided to small and sick newborns is a quality improvement initiative that helps to identify the modifiable gaps in newborn care (BMC Pregnancy Childbirth 14: 280, 2014). The aim of this work was to identify literature on modifiable factors in the care of newborns in the newborn units in health facilities in low-middle-income countries (LMICs). We also set out to design a measure of the quality of the perinatal and newborn audit process.

Methods
The scoping review was conducted using the methodology outlined by Arksey and O’Malley and refined by Levac et al, (Implement Sci 5:1-9, 2010). We reported our results using the PRISMA Extension for Scoping Reviews (PRISMA-ScR) guidelines. We identified seven factors to ensure a successful audit process based on World Health Organisation (WHO) recommendations which we subsequently used to develop a quality of audit process score.

Data sources
We conducted a structured search using PubMed, CINAHL, EMBASE, LILACS, POPLINE and African Index Medicus.

Study selection
Studies published in English between 1965 and December 2019 focusing on the identification of modifiable factors through clinical or mortality audits in newborn care in health facilities from LMICs.

Data extraction
We extracted data on the study characteristics, modifiable factors and quality of audit process indicators.

Results
A total of six articles met the inclusion criteria. Of these, four were mortality audit studies and two were clinical audit studies that we used to assess the quality of the audit process. None of the studies were well conducted, two were moderately well conducted, and four were poorly conducted. The modifiable factors were divided into three time periods along the continuum of newborn care. The period of newborn unit care had the highest number of modifiable factors, and in each period, the health worker related modifiable factors were the most dominant.

Conclusion
Based on the significant number of modifiable factors in the newborn unit, a neonatal audit tool is essential to act as a structured guide for auditing newborn unit care in LMICs. The quality of audit process guide is a useful method of ensuring high quality audits in health facilities.
Demographic, practice and clinical management characteristics of osteopaths referring to podiatrists: secondary analysis of a nationally representative sample of Australian osteopaths
Authors: Fleischmann Michael , Vaughan Brett , Bird Adam , Grace Sandra , Fitzgerald Kylie , McLeod Gopi 
Published: February 2022

Abstract
Background
Interprofessional care is paramount in contemporary healthcare practice. How different professions interact, and the characteristics of those practitioners who practice in an interprofessional way are rarely described in the literature. The aim of the current work was to identify the demographic, practice and clinical management characteristics of Australian osteopaths who report referring to podiatrists.

Methods
The study was a secondary analysis of data from the Osteopathy Research and Innovation Network (ORION). Inferential statistics were generated to identify statistically significant demographic, practice and clinical management characteristics associated with referrals to podiatrists by Australian osteopaths.

Results
Nine-hundred and ninety-two Australian osteopaths responded to the questionnaire. Sending referrals to a podiatrist was reported by 651 participants (65.6%). Female Australian osteopaths were less likely to report referring to podiatrists compared to male osteopaths (OR 0.76, 95%CI 0.59–0.99). Australian osteopaths who reported referring to podiatrists were more likely to report receiving referrals from podiatrists (OR 9.75, 95%CI 6.98–13.61), use orthopaedic testing in patient assessment (OR 7.62, 95%CI 2.82–20.60), and often treat patients with postural disorders (OR 1.71, 95%CI 1.03–2.26), compared to osteopaths who do not refer to podiatrists.

Conclusion
This study provides initial evidence for the referral relationship between Australian osteopaths and podiatrists. Further work could explore the nature of these referrals, including the complaints resulting in referral and outcomes of care. This information will be useful to those involved in health policy development and the professions advocating for their role in the wider healthcare system.
A myxozoan genome reveals mosaic evolution in a parasitic cnidarian
Authors: Guo Qingxiang , Atkinson Stephen D. , Xiao Bin , Zhai Yanhua , Bartholomew Jerri L. , Gu Zemao 
Published: February 2022

AbstractBackgroundParasite evolution has been conceptualized as a process of genetic loss and simplification. Contrary to this model, there is evidence of expansion and conservation of gene families related to essential functions of parasitism in some parasite genomes, reminiscent of widespreadmosaic evolution—where subregions of a genome have different rates of evolutionary change. We found evidence of mosaic genome evolution in the cnidarianMyxobolus honghuensis, a myxozoan parasite of fish, with extremely simple morphology.ResultsWe comparedM. honghuensiswith other myxozoans and free-living cnidarians, and determined that it has a relatively larger myxozoan genome (206 Mb), which is less reduced and less compact due to gene retention, large introns, transposon insertion, but not polyploidy. Relative to other metazoans, theM. honghuensisgenome is depleted of neural genes and has only the simplest animal immune components. Conversely, it has relatively more genes involved in stress resistance, tissue invasion, energy metabolism, and cellular processes compared to other myxozoans and free-living cnidarians. We postulate that the expansion of these gene families is the result of evolutionary adaptations to endoparasitism.M. honghuensisretains genes found in free-living Cnidaria, including a reduced nervous system, myogenic components, ANTP class Homeobox genes, and components of the Wnt and Hedgehog pathways.ConclusionsOur analyses suggest that theM. honghuensisgenome evolved as a mosaic of conservative, divergent, depleted, and enhanced genes and pathways. These findings illustrate that myxozoans are not as genetically simple as previously regarded, and the evolution of some myxozoans is driven by both genomic streamlining and expansion.
An artificial intelligence method to assess the tumor microenvironment with treatment outcomes for gastric cancer patients after gastrectomy
Authors: Chen Tao , Li Xunjun , Mao Qingyi , Wang Yiyun , Li Hanyi , Wang Chen , Shen Yuyang , Guo Erjia , He Qinglie , Tian Jie , Zhu Mansheng , Wu Jing , Liang Weiqi , Liu Hao , Yu Jiang , Li Guoxin 
Published: February 2022

Abstract
Background
The tumor microenvironment (TME) plays an important role in the occurrence and development of gastric cancer (GC) and is widely used to assess the treatment outcomes of GC patients. Immunohistochemistry (IHC) and gene sequencing are the main analysis methods for the TME but are limited due to the subjectivity of observers, the high cost of equipment and the need for professional analysts.

Methods
The ImmunoScore (IS) was developed in the TCGA cohort and validated in GEO cohorts. The Radiomic ImmunoScore (RIS) was developed in the TCGA cohort and validated in the Nanfang cohort. A nomogram was developed and validated in the Nanfang cohort based on RIS and clinical features.

Results
For IS, the area under the curves (AUCs) were 0.798 for 2-year overall survival (OS) and 0.873 for 4-year overall survival. For RIS, in the TCGA cohort, the AUCs distinguishing High-IS or Low-IS and predicting prognosis were 0.85 and 0.81, respectively; in the Nanfang cohort, the AUC predicting prognosis was 0.72. The nomogram performed better than the TNM staging system according to the ROC curve (all P < 0.01). Patients with TNM stage II and III in the High-nomogram group were more likely to benefit from adjuvant chemotherapy than Low-nomogram group patients.

Conclusions
The RIS and the nomogram can be used to assess the TME, prognosis and adjuvant chemotherapy benefit of GC patients after radical gastrectomy and are valuable additions to the current TNM staging system. High-nomogram GC patients may benefit more from adjuvant chemotherapy than Low-nomogram GC patients.
Revealing potential anti-fibrotic mechanism of Ganxianfang formula based on RNA sequence
Authors: Liu Zongyi , Xiang Huanyu , Xiang Dejuan , Xiao Shuang , Xiang Hongyan , Xiao Jing , Ren Hong , Hu Peng , Liu Huabao , Peng Mingli 
Published: February 2022

Abstract
Background
Ganxianfang (GXF) formula as a traditional Chinese medicine (TCM) is used for liver fibrosis in clinical practice while its mechanism is unclear. The aim of this study is to explore the molecular mechanism of GXF against CCl4-induced liver fibrosis rats.

Methods
Detected the main compounds of GXF by UPLC-MS/MS. Evaluated the efficacy of GXF (1.58, 3.15, 4.73 g/kg/day) and Fuzheng Huayu (FZHY, positive control, 0.47 g/kg/day) through serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) levels and histopathological changes. Explored the underlying mechanisms by integrating our total liver RNA sequencing (RNA-seq) data with recent liver single-cell sequencing (scRNA-seq) studies. Verified potential pharmacodynamic substances of GXF by hepatic stellate cell (HSC)-T6 line.

Results
Main compounds were identified in GXF by UPLC-MS/MS, including baicalin, wogonoside and matrine etc. With GXF-high dose treatment, the elevation of ALT and AST induced by CCl4 were significantly reduced, and the protective effect of GXF-high dose treatment was better than FZHY. Liver histopathological changes were alleviated by GXF-high dose treatment, the ISHAK scoring showed the incidence of liver cirrhosis (F5/F6) decreased from 76.5 to 55.6%. The results of liver hydroxyproline content were consistent with the histopathological changes. RNA-seq analysis revealed the differential genes (DEGs) were mainly enriched in ECM-receptor interaction and chemokine signaling pathway. GXF effectively inhibited collagen deposition and significantly downregulated CCL2 to inhibit the recruitment of macrophages in liver tissue. Integrating scRNA-seq data revealed that GXF effectively inhibited the expansion of scar-associated Trem2+CD9+ macrophages subpopulation and PDGFRα+PDGFRβ+ scar-producing myofibroblasts in the damaged liver, and remodeled the fibrotic niche via regulation of ligand-receptor interactions including TGFβ/EGFR, PDGFB/PDGFRα, and TNFSF12/TNFRSF12a signaling. In vitro experiments demonstrated that baicalin, matrine and hesperidin in GXF inhibited the activation of hepatic stellate cells.

Conclusions
This study clarified the potential anti-fibrotic effects and molecular mechanism of GXF in CCl4-induced liver fibrosis rats, which deserves further promotion and application.
The social contagion of temporal discounting in small social networks
Authors: Bixter Michael T. , Luhmann Christian C. 
Published: February 2022

AbstractDecisions often require a tradeoff between immediate and long-term gratification. How individuals resolve such tradeoffs reflects constructs such as temporal discounting, the degree that individuals devalue delayed rewards. Recent research has started to focus on temporal decisions made in collaborative contexts (e.g., dyads, small groups). Results suggest that directly interacting with others leads to revisions in preferences, such that decision makers become more similar to their collaborative partners over time (e.g., more patient following collaboration with a patient other). What remains to be seen is whether this social influence extends to indirect social effects, such as when an individual influences another’s preferences through a shared collaborative partner. In the current study, the focus was on decisions regarding hypothetical monetary rewards. Groups of three participated in a collaborative decision-making chain, in which network member X collaborated with member Y, who then subsequently collaborated with member Z. Though network members X and Z never directly interacted, a significant indirect link was observed between member X’s pre-collaborative decision preferences and member Z’s post-collaborative decision preferences. These results demonstrate that temporal decision preferences can be transmitted through intervening connections in a small social network (i.e., social contagion), showing that indirect social influence can be empirically observed and measured in controlled environments.
Effect of extraction technique, solvent polarity, and plant matrix on the antioxidant properties of Chrysophyllum albidum G. Don (African Star Apple)
Authors: Akinmoladun Afolabi C. , Falaiye Olubusola Elizabeth , Ojo Olubukola Benedicta , Adeoti Abimbola , Amoo Zainab Abiola , Olaleye Mary Tolulope 
Published: February 2022

Abstract
Background
Chrysophyllum albidum is a well-known medicinal plant in Africa and has many medicinal properties. This study investigated the effect of sonication, solvent polarity (acetone and ethanol), and plant matrix (bark and seeds) on the antioxidant property of C. albidum. The bark of C. albidum was subjected to sonication and soaking with acetone to evaluate the effect of sonication on the antioxidant property, and C. albidum bark and seeds were subjected to ultrasonic-assisted extraction of acetone and ethanol to evaluate the effect of solvents and plant matrix on the antioxidant property of C. albidum. The phytochemical composition, total flavonoid content, total antioxidant activity, total phenol content, lipid peroxidation (LPO) inhibition activity, nitric oxide, and 2, 2-diphenyl-1-picrylhydrazyl radicals scavenging activities were evaluated in all extracts.

Results
Sonication increased the percentage yield of extracts compared to maceration. Flavonoids and terpenoids were present, while saponins were absent in all extracts evaluated. Ultrasound-assisted extraction increased C. albidum antioxidant property compared to maceration. Ethanol was the most suitable solvent for C. albidum bark, while acetone was the most suitable solvent for C. albidum seeds. C. albidum bark extracts were most active as free radical scavengers, while the seed extracts were most active as inhibitors of lipid peroxidation.

Conclusions
Extraction technique, extraction solvent, and plant matrix significantly affect the antioxidant properties of C. albidum. This study indicates that the selection of an effective extraction process for medicinal plants depends on the phytochemical compound responsible for the biological activity of interest.
Significance of promoter methylation of multiple tumor suppressor genes in hepatocellular carcinoma
Authors: Tahoon Alaa , El-Khateeb Doaa , Mosbeh Asmaa , Tantawy El Sayed Ibrahim , Khalil Ashraf 
Published: February 2022

AbstractBackgroundMethylation of the promoter at CpG islands is a mechanism of silencing tumor suppressor genes and therefore enhances cancer progression. The study aimed to examine promoter methylation frequencies of five tumor suppressor genes in hepatocellular carcinoma and their implication on the first-year outcome of surgical resection of the tumor. Fifty specimens of hepatocellular carcinoma and the adjacent non-tumorous liver tissue were collected from the surgically resected hepatic tumor. The status of promoter methylation of tumor suppressor genes RASSF1A, CHFR, MGMT, GSTP1, and hMLH1 was investigated using methylation-specific polymerase chain reaction.ResultsThe frequency of promoter methylation of these tumor suppressors genes (TSG) genes in hepatocellular carcinoma was significantly higher than non-tumorous tissue all, P < 0.05, with a methylation rate of 80% in RASSF1A, 70% in CHFR, 46% in GSTP1, 56% in MGMT, and 10% in hMLH1. Methylation of RASSF1A, CHFR, and MGMT promoter genes was significantly associated with decreased first-year postoperative survival and increased recurrence of hepatocellular carcinoma, P < 0.05.ConclusionMethylated RASSF1A, CHRF, and MGMT promoters indicated poor prognosis among patients with hepatocellular carcinoma and may serve as potential prognostic indicators in patients with hepatocellular carcinoma.
Determining shear failure gradient and optimum drilling mud window in the Ourhoud oil field, Berkine Basin, Algeria
Authors: Baouche Rafik , Sen Souvik , Feriel Hadj Arab 
Published: February 2022

Optimum drilling mud window provides a workable downhole mud pressure range to prevent formation fluid influx, borehole instabilities, and fluid loss into the formation while drilling, and this can be achieved by a comprehensive geomechanical modeling using well data. We have integrated the wireline logs, drilling data, and measured downhole data to assess the vertical stress, pore pressure, minimum horizontal stress, and shear failure (SF) gradient of the 3400 m thick Mesozoic and Paleozoic succession in the Ourhoud field, Berkine Basin. We have interpreted the hydrocarbon pressure gradient in the Triassic Argilo-Greseux Inferieur reservoir as 0.32 psi/ft and found the overburden shales to be hydrostatically pressured (0.46 psi/ft). Poisson’s ratio-based minimum horizontal stress has a 0.73–0.80 psi/ft gradient, whereas frictional faulting theory provides a lower limit of 0.66 psi/ft. We have observed massive wash outs in the caliper logs against the Cretaceous shales, which is more prone to compressive failures. To address this wellbore failure, we have modeled SF gradient by Mohr-Coulomb rock failure criteria and compared the results with the mud pressure used in drilling. We have inferred that at least 10.5 ppg drilling mud weight is required to prevent such wellbore instabilities in the Mesozoic shales, whereas the minimum allowable mud weight for the Carboniferous shale is 10 ppg. Based on the interpreted pressure gradients, we have recommended an optimum downhole drilling window for the Ourhoud field, which will be helpful to deliver stable wellbores in future drilling campaigns.
Influence of pedestrian psychology on evacuation dynamics with the guide of emergency signage
Authors: Jiang Qiao , Zhou Ying , Zhang Lei , Li Kun , Li Huan 
Published: February 2022

Abstract
How to simulate the true pedestrians' psychology who face an emergency is an important issue that decides evacuation efficiency. Pedestrian psychology during the evacuation process is complex, abstract and difficult to quantify directly. Based on the typical social force model, this paper improves the pedestrian direction selection procedure and introduces a “weight factor” to adjust the degree of contribution of “the density factor” and “the distance factor” to pedestrians' choice of moving direction. In this model, pedestrians can not only roughly estimate the distance between themselves and each exit, but also judge the degree of crowd gathering near each exit by virtue of the electronic display screen in the center of the cross-shaped aisle. Simulation results show that, for a configuration with multi-rooms and multi-exits, when the pedestrian number reaches a certain level, only considering the distance factor will lead to uneven distribution of the crowd, therefore inducing obvious congestion. On the other hand, concentrating too much on the density factor makes pedestrians hesitate to choose the appropriate exit. In conclusion, we should comprehensively consider the impact of the “density factor” and the “distance factor”, which helps to provide feasible evacuation strategies for emergency situations and to improve both evacuation efficiency and exit utilization.