bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel biomarker NEDD1 and its analysis in hepatocellular carcinoma
Authors: Zhou Xinmin , Ding Xiaopeng , Yu Jiahao , Shi Xin , Li Kangwei , Ma Shuoyi , Ren Pengwei , Xuan Guoyun , Han Ying 
Published: January 2022

Abstract
Background: NEDD1 (NEDD1 Gamma-Tubulin Ring Complex Targeting Factor) plays a crucial impact in regulating cell cycle and the development of scirrhous gastric cancer. However, the role of NEDD1 hasn’t been reported in hepatocellular carcinoma (HCC) so far. The aim of this research is to explore the role of NEDD1 on the development and prognosis of HCC. Methods: All methods were performed in accordance with the relevant guidelines and regulations. HCC-related data were download from The Cancer Genome Atlas (TCGA) database. Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and gene set enrichment analysis (GSEA) were conducted by the LinkedOmics database. Results: The expression of NEDD1 has significant difference between tumor and adjacent normal tissues in HCC (P＜0.01). We also found that NEDD1 was an independent risk factor in HCC patients (HR 1.643, 95%CI 1.125–2.398; P = 0.01). The study also demonstrated that NEDD1 expression was significantly relevant to the expression of several immune checkpoint genes, including CTLA-4, PD-L1 and PD-1. GSEA revealed that Cell cycle, MicroRNAs in cancer and Ribosome pathways were significantly enriched in NEDD1 overexpression phenotype. By integrating NEDD1 with other relevant factors, we constructed the prognostic nomogram to help the improvement of the prognosis for patients with HCC. The data from the International Cancer Genome Consortium (ICGC) database were used as an independent external validation of our prognostic model. Conclusion: The expression level of NEDD1 was negatively correlated to the prognosis of HCC patients and it may be a promising therapeutic target of HCC, which probably be able to predict the efficacy of immunotherapy for HCC patients.
Practices And Challenges of Cultural Heritage Conservation In Historical And Religious Heritage Sites: Evidence From North Shewa Zone, Amhara Region
Authors: Mekonnen Habtamu , Bires Zemenu , Berhanu Kassegn 
Published: January 2022

Abstract
Cultural heritage are precious resources that showed the past human legacy, depicts present and future way of life as well as cultural values of a society, and it enhances solidarity and social integration of communities. However, due to natural catastrophes and anthropogenic factors, the sustainability of cultural heritage is questionable and requires scientific investigations to uncover the major problems affecting the continual of heritage attractions so that the panacea for this can be devised. Therefore, this study is designed to investigate the practices and challenges of cultural heritage conservations in North Shoa Zone, Central Ethiopia. Methodologically, the research employed a mixed research approach and cross-sectional descriptive and explanatory research design. While systematic random sampling technique is applied to select samples from local communities, purposive sampling was designed to choose interviewees. The target population of the study encompasses local communities, government authorities, and culture and tourism office experts of North Shoa Zone and respective districts. The actual and valid sample size of the study is 208. The findings of the study revealed that the cultural heritages are not safeguarded from damaging activities indicating a poor status of conservation. The major conclusion sketched from the study is the principal factors affecting heritage conservation are lack of proper management, monitoring and evaluation, lack of funds and stakeholder involvement, urbanization, settlement programs and agricultural practice, poor government concern and professional commitment, poor attitude towards cultural heritage and lack of community concern, vandalism and illicit trafficking, low promotions of cultural heritage, and natural catastrophes like invasive intervention, climate change (humidity and frost, excessive rainfall and flood, heat from the sun). The study implied that the sustainability of cultural heritage is endanger unless conservation practice is supported by conservation guidelines, heritage site management plans and research outputs, stakeholders’ integration, and community involvement. Most importantly, the study recommends the integration of heritage conservation and sustainable development, and the promotion of conservation is a way of achieving economic and social sustainability.
Application of Response Surface Methodology and Artificial Neural Network for The Preparation of Fe-Loaded Biochar for Enhanced Cr(VI) Adsorption and Its Cr(VI) Adsorption Characteristics in an Aqueous Solution
Authors: Kang Jin-Kyu , Seo Eun-Jin , Lee Chang-Gu , Jeong Sanghyun , Park Seong-Jik 
Published: January 2022

Abstract
In this study, we optimized and explored the effect of the conditions for synthesizing Fe-loaded food waste biochar (Fe@FWB) for Cr(VI) removal using the response surface methodology (RSM) and artificial neural network (ANN). The pyrolysis time, temperature, and Fe concentration were selected as the independent variables, and the Cr(VI) adsorption capacity of Fe@FWB was maximized. RSM analysis showed that the p-values of pyrolysis temperature and Fe concentration were less than 0.05, indicating that those variables were statically significant, while pyrolysis time was less significant due to its high p-value (0.2830). However, the ANN model results showed that the effect of pyrolysis time was more significant on Cr(VI) adsorption capacity than Fe concentration. The optimal conditions, determined by the RSM analysis with a lower sum of squared error than ANN analysis, were used to synthesize the optimized Fe@FWB (Fe@FWB-OPT) for Cr(VI) removal. From the equilibrium model fitting, the Langmuir model showed a better fit than the Freundlich model, while the Redlich–Peterson isotherm model overlapped. The Cr(VI) sorption capacity of Fe@FWB-OPT calculated from the Langmuir model was 377.71 mg/g, high enough to be competitive to other adsorbents. The kinetic Cr(VI) adsorption was well described by the pseudo-second-order and Elovich models. The XPS results showed that Cr adsorbed on the surface of Fe-FWB-OPT was present not only as Cr(VI) but also as Cr(III) by the reduction of Cr(VI). The results of Cr(VI) adsorption by varying the pH indicate that electrostatic attraction is a key adsorption mechanism.
Intestinal flora and inflammation in acute coronary syndromes
Authors: Wang Hao , Ma Huiyan , Yang Libo , Yan Ning , Zhang Hua , Zhang Xiaoxia , Jia Shaobin 
Published: January 2022

Abstract
Background: Acute coronary syndromes (ACS) is closely associated with chronic low-grade inflammation and gut microbiome composition. However, the composition and functional capacity of the gut microbiome in relation to ACS have not been systematically examined. Results: we perform a metagenome-wide association study on stools and plasma from 66 individuals with ACS and 46 healthy controls (HC). We then compared gut microbial composition using 16S ribosomal RNA gene sequencing in fecal samples to detect species with differential abundance between 2 groups. We reported that the altered composition of gut microbiota was associated with ACS and exacerbated inflammatory status. Moreover, parameters in ACS including body weights (BWs), low-density lipoprotein (LDL), triglyceride (TG), total cholesterol (TC), C-reactive protein (CRP) and high homocysteine (HCY) were elevated; whereas high-density lipoprotein (HDL) was decreased. pro-inflammatory interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, monocyte chemoattractant protein-1(MCP-1) and lipopolysaccaride (LPS) in ACS were increased respectively. The results of 16S rRNA sequencing and analysis displayed that the overall community of gut microbiota in ACS was notably changed mainly through increasing the abundance of Bacteroidetes, Verrucomicrobia, Proteobacteria Parabacteroide, Unidentified_Enterobacteriaceae, Subdoligranulum, Akkermansia, Alistipes, Streptococcus, Paraprevotella as well as decreasing Subdoligranulum, Roseburia, Faecalibacterium, Blautia, Agathobacter, Anaerostipes, Bifidobacterium. Further analysis showed that there was a significant correlation between the above differences in gut microbiota and inflammatory factors. Conclusions: Our data represent a comprehensive resource for further investigations on the role of the gut microbiome in promoting or preventing ACS.
Effects of Acculturation of Eating Disorder Prevalence Across Generations of Asian American Immigrants
Authors: Devata Tejas , Devata Veda , Chiu Bianca , Lin Karen , Garg Gouri , Thomas Edward , Lee Jennifer , Tan Cynthia , Ongking Julia , Xu Katherine , Pannase Shreyas , Dadas Dada 
Published: January 2022

Abstract
The growing prevalence of eating disorders in the United States is expected to stay on the rise and is attributed to the various changes in lifestyle and diet in the past two decades. This research paper analyzed the extent that acculturation, the absorption of one dominant culture over another, impacts the rate of eating disorders in the United States and whether the variable is significant. This study investigates various ethnic and cultural variables that can influence immigrants’ and children of immigrants’ susceptibility to eating disorders. Specifically, the study analyzes the extended exposure to American cultural standards of physical attractiveness, generally differing from their own. The data will be collected by conducting surveys amongst a randomly sampled first/second-generation Asian-American population in the United States. The study participants will be screened using the Eating Disorder Examination Questionnaire (EDE-Q) in addition to an Acculturation Index, specifically the General Ethnicity Questionnaire (GEQ). Researchers expect to observe a positive correlation between the extent of acculturation and the prevalence of eating disorders amongst the sample population, in this case, being first and second-generation Asian Americans residing in the United States. Results disproved this hypothesis, analyzing 131 responses from around the United States, acculturation had no significant correlation to eating disorder prevalence in the study group. Significant trends were found between levels of acculturation in each generation, second generation Asian Americans being significantly more acculturated. Second generation immigrants were however had lower global EDE-Q scores compared to their first generation counterparts.
Gallic acid enhances the antitumor activity of Icotinib hydrochloride in non-small cell lung cancer via of Hippo-YAP signaling pathway in vitro and in vivo
Authors: Wang Dapeng , Guo Yinghua , Liu Yuan , Sa Rengerile , Wang Junfeng , Zhang Bin , Bai Po , Su Xiaolei , Zhang Xuelin , Zhang Yongqing , Liu Changting 
Published: January 2022

Abstract
Icotinib hydrochloride (IH) is a small molecular TKI independently developed in China. As the first-line anti-tumor agent, is widely used for treatment of non-small cell lung cancer (NSCLC). Gallic acid (GA), a natural plant extract, is reported that it has a variety of pharmacological and biological properties. GA is a active natural phenolic extracted from Tannins that has been shown to exhibit anticancer activities on various types of tumors. Here, we reported that GA was capable of sensitizing A549/PC9 cells to IH by enhancing apoptosis. Mechanistic analyses indicated that IH-induced caspase-3-dependent apoptosis was elevated in the presence of GA through activating extracellular signal-regulated Hippo-YAP pathway. Furthermore, GA also promoted IH-induced cytotoxic, downregulated expression of p-YAP and caspase-3. In vivo, the co-treatment of IH and GA notably reduced the tumor size when compared with IH treatment alone. Notably, GA significantly reduced the toxicity generated by IH in tumor-bearing mice. This study identifies the unique role of GA enhance IH sensitivity through apoptosis, and suggests that combined IH and GA might be a novel therapeutic strategy for patients with NSCLC.
Attosecond Optical Switching
Authors: Hui Dandan , Alqattan Husain , Zhang Simin , Pervak Vladimir , Chowdhury Enam , Hassan Mohammed 
Published: January 2022

Abstract
Modern electronics are founded on switching the electric signal by radio frequency (RF) electromagnetic fields on the nanosecond timescale, limiting the information processing to the gigahertz speed. Recently, optical switches have been demonstrated using terahertz and ultrafast laser pulses to control the electric signal and enhance the switching speed to the picosecond and a few hundred femtoseconds time scale1-13. Here, we exploit the reflectivity modulation of a dielectric system in a strong light field to demonstrate the optical switching (ON/OFF) with attosecond time resolution. Moreover, we present the capability of controlling the optical switching signal with complex synthesized fields of ultrashort laser pulses for data binary encoding. This work paves the way for establishing optical switches and light-based electronics with peta- and exahertz speeds, several orders of magnitude faster than the current semiconductor-based electronics, opening a new realm in information technology, optical communications, and photonic processor technologies.
Waterborne Exposure to Avobenzone and Octinoxate Induces Thyroid Endocrine Disruption in Wild-Type and Thrαa-/- Zebrafish Larvae
Authors: Ka Yujin , Ji Kyunghee 
Published: January 2022

Abstract
Avobenzone and octinoxate are frequently used as organic ultraviolet filters, and these chemicals are widely detected in water. This study evaluated the potential of avobenzone and octinoxate to disrupt thyroid endocrine system in wild-type and thyroid hormone receptor alpha a knockout (thrαa−/−) zebrafish embryos/larvae. Following a 120 h exposure to various concentrations of avobenzone and octinoxate, larvae mortality and developmental toxicity in wild-type and thrαa−/− fish were assessed. Triiodothyronine (T3) and thyroxine (T4) levels as well as transcriptional levels of ten genes associated with the hypothalamus-pituitary-thyroid (HPT) axis were measured in wild-type fish. Significantly lower larvae survival rate in thrαa−/− fish exposed to ≥ 3 µM avobenzone and octinoxate suggests that the thyroid hormone receptor plays a crucial role in the toxic effects of avobenzone and octinoxate. A significant increase in the deio2 gene level in avobenzone-exposed zebrafish supports the result of an increased ratio of T3 to T4. Significant decrease of T4 level with upregulation of trh, tshβ, and tshr genes indicates feedback in the hypothalamus and pituitary gland to maintain hormonal homeostasis. Our observation indicates that exposure to avobenzone and octinoxate affects the feedback mechanisms of the HPT axis.
Morphological And Morphometrical Aspects of The Auditory Ossicles In Roe Deer (Capreolus Capreolus)
Authors: Cristian Martonos , Gudea Alexandru , Gianluca D’Amico , Florin Stan 
Published: January 2022

Abstract
The study provides a series of distinctive aspects of the auditory ossicles alongside comparative morphometric data, bringing facts in respect to morphology and morphofunctionality of the auditory ossicles in this little-studied species. The most important features noted are evident conical shape of muscular process of malleus and triangular aspect of the handle of malleus. For the incus, a short body of the bone and the direct continuation, with no clear distinction as an individualized piece, for the lenticular process. As for the stapes, the clear profiling of the muscular tubercle for the stapedial muscle and elliptic shape of the foot of the stapes is noted. A series of comparative measurements and indices are also calculated in the attempt of profiling differences from the domestic counter species- sheep and goat.
Influence of Surface Roughening of the Raw Material on the Lap Shearing Strength and Failure Behavior of Adhesively Bonded Aluminum Joints
Authors: Liu Jianpeng , Wang Wei , Yan Yong , He Hong , Xue Zhigang , Xu Congchang , Li Luoxing 
Published: January 2022

Abstract
Surface roughening of the substrates before bonding plays a significant effect on improving the mechanical performance of the adhesively bonded joints, which are prevalently used for light-weighting vehicle bodies. In this study, the influence of surface roughening on the lap shearing strength and failure behavior of adhesively bonded aluminum sheet joints was investigated. Sandpaper grinding was employed for surface roughening, methods such as tensile testing microstructure observation, etc., was employed for evaluating the performance of the joints. The results showed that the lap shearing strength of adhesively bonded joints increased and then decreased with the surface roughness of the aluminum substrate. The maximum shearing strength of the joint bonded with grinded substrates was 30.4 MPa which was improved by 57.5% compared to that produced with un-grinded substrates. However, over roughening is harmful. When the surface roughness was too large, the failure mode of the joint turned from the mixed failure mode to interfacial failure mode, which decreased the strength of the joints. Related mechanisms were demonstrated. When the substrate surface was coarsened, the bonding area and the wettability of the adhesive on the surface were both increased, which promoted the beneficial mechanical interlock effect between the adhesive and the substrate. However, when the substrate surface was over roughened, defects such as voids, insufficient infiltrating of the adhesive, etc., were induced, which apparently increased the proportion of the interfacial failure area.
Water Supply-Demand Situation Analysis Based on a Hierarchical Index System
Authors: Wang Ting , You Jinjun , Ma Zhenzhen , Xiao Ping 
Published: January 2022

Abstract
In this study, a hierarchical index system is proposed for analysis of water supply-demand situation, which can overcome the shortcomings of complicated analysis and ambiguous decision, associated with the use of a single index like water shortage or water shortage ratio. Five indices responsible for water supply-demand imbalance are considered on both water resources supply and demand sides, and corresponding regulation strategies are proposed to restore water supply-demand balance by improving the water supply capacity of conventional and non-conventional water resources, improving water-use efficiency, and controlling the social and economic development scale. The proposed hierarchical index system has been successfully applied to the analysis of the water supply-demand situation in Qingdao. The results reveal that in order to improve the severe water supply-demand situation in Qingdao, it is necessary to divert water from other regions and improve agricultural water-use efficiency. This study may provide insights into the design and preparation of inter-basin or cross-regional water diversion projects and the formulation of annual water diversion plans.
SNTA1-deficient human cardiomyocytes are associated with increased structural components, calcium handling disorder, and shorter field potential duration
Authors: Dong Tao , Zhao Yan , Jin Hai-Feng , Shen Lei , Si Long-Long , Chen Li 
Published: January 2022

Abstract
Background

α-1-syntrophin (SNTA1), a protein encoded by SNTA1, is highly expressed in human cardiomyocytes. Mutations in SNTA1 are associated with arrhythmia and cardiomyopathy. Previous research on SNTA1 has been based on nonhuman cardiomyocytes. Our study was designed to identify phenotype of SNTA1-deficient using human cardiomyocytes.
Methods

SNTA1 was knocked out in H9 cell line using CRISPR-Cas9 system. H9SNTA1KO cells were then induced to differentiate into cardiomyocytes using small molecule inhibitors. The phenotypic discrepancies associated with SNTA1-deficient cardiomyocytes were investigated.
Results

SNTA1 was truncated in PH1 domain by a stop codon (TGA) using CRISPR-Cas9 system. SNTA1-deficient did not affect the pluripotency of H9SNTA1KO, and they retain their in vitro ability to differentiate into cardiomyocytes. However, H9SNTA1KO derived cardiomyocytes exhibited increased structural components, weak calcium transient intensity, low levels of calcium in sarcoplasmic reticulum, lower cardiac contractility, and shorter field potential duration. Early treatment of SNTA1-deficient cardiomyocytes with ranolazine improved the calcium transient intensity and cardiac contractility.
Conclusions

SNTA1-deficient cardiomyocytes can be used to research the etiology, pathogenesis, and potential therapies for myocardial diseases. The SNTA1-deficient cardiomyocyte model suggests that the maintenance of cardiac calcium homeostasis is a key target in the treatment of myocardial-related diseases.
ADRB2 Expression Predicts the Clinical Outcomes and is Associated with Immune Cells Infiltration in Lung Adenocarcinoma
Authors: Ji Lingyun , Xu Fei , Zhang Jingtao , Chen Weida , Yin Xi , Wang Qingqing , Chen Xiubao , Li Xin , Guo Minghao , Chen Zetao 
Published: January 2022

Abstract
The gene encoding beta2-adrenergic receptor (β2-AR), adrenoceptor beta 2 (ADRB2), has been reported to closely associated with various cancers. However, its role in lung adenocarcinoma (LUAD) remains controversial. This research shed light on the prognostic value of ADRB2 in LUAD and further explored its association with immune cell infiltration. ADRB2 was significantly decreased in LUAD. ADRB2 expression in LUAD was significantly correlated with gender, smoking status, T classification, lymph node metastasis, and pathologic stage. Patients in the low ADRB2 expression group presented with significantly poorer overall survival (OS) and disease-specific survival (DSS). Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) results showed that ADRB2 participates in immune response. The expression of ADRB2 was positively correlated with the infiltration level of most immune cells. Notably, ADRB2 is involved in LUAD progression partly by regulating the immune microenvironment, which may potentially serve as a significant prognostic biomarker as well as a potential drug target.
PTPROt Enhances the Sensitivity of Multiple Myeloma to BP-1-102 and Bortezomib
Authors: Li Zixuan , Liu Yong , Xie Yan , Yuan Lei , An Gangli , Fu Chengcheng , Yang Lin 
Published: January 2022

Abstract
BackgroundMultiple myeloma (MM) is an incurable plasma cell malignancy for which novel treatment options are urgently required. STAT3 overexpression is associated with poor prognosis of MM and could enhance chemotherapy resistance of MM cells. Therefore, targeting the expression and/or activity of STAT3 may represent a potential treatment strategy for MM. Methods and resultsThis study aimed to examine the effects of protein tyrosine phosphatase receptor type O (PTPROt) overexpression and BP-1-102, an inhibitor of STAT3, on MM cell growth and survival. The results showed that PTPROt overexpression suppressed MM cell progression by inhibiting STAT3 phosphorylation, and that BP-1-102 inhibited the proliferation, migration, and invasion of MM cell lines in a dose-dependent manner. We also found that BP-1-102 hampered STAT3 phosphorylation and induced apoptosis in vitro, and inhibited the growth of H929 and MM1S xenografts in vivo. Furthermore, the combination of BP-1-102 and bortezomib (BTZ) was more efficacious at killing MM cells than BP-1-102 or BTZ alone in vitro and in vivo. Notably, PTPROt also enhanced the sensitivities of multiple myeloma cell lines, primary myeloma cells, and H929-PTPROt xenograft tumors to BP-1-102 and BTZ treatments. ConclusionsThe findings of the current study strongly indicated that STAT3 could be a promising therapeutic target for the treatment of MM.