bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytoglobin regulates nitric oxide-dependent cilia motility and organ laterality in development
Authors: Corti Paola , Rochon Elizabeth , Xue Jianmin , Saydmohammed Manush , Smith Caroline , Zahid Maliha , DeMartino Anthony , Lo Cecilia , Tsang Michael , Tejero Jesus , Gladwin Mark 
Published: October 2022

Abstract
Cytoglobin is a highly conserved redox sensor heme protein with unknown physiological function. Unexpectedly, genetic deletion of zebrafish cytoglobin (cygb2) causes cardiac and gastro-intestinal tract laterality defects, modeling Kartagener’s syndrome, which in humans is associated with low airway epithelial nitric oxide (NO) production. Cygb2 co-localized with cilia and the NO synthase Nos2b in the Kupffer’s vesicle (KV) laterality organ and the structure and function of cilia were disrupted in cygb2 mutants, abolishing fluid flow within the KV. Abnormal ciliary function and organ laterality was phenocopied by depletion of nos2band gucy1a, the canonical NO receptor soluble guanylate cyclase (sGC) homolog in fish, and rescued by exposing cygb2 mutant embryos to an NO donor or an sGC stimulator and with over-expression of nos2b. Consistent with a conserved role in the modulation of ciliary function, cytoglobin knock out in the mouse also impaired airway epithelial ciliary structure and beat frequency. This is the first evidence that cytoglobin is essential for normal development stage-specific NO signaling, ciliogenesis and cilia motility, required for the establishment of left-right patterning.
Correlation of preoperative anemia subtypes with tumor characteristics, systemic inflammation and immediate postoperative outcomes in gastrointestinal cancer patients – a prospective observational study
Authors: Mahalingam Sudharshan , Anandhi Amaranathan , Sureshkumar Sathasivam , Udayakumar Karthikrajan Parasuraman 
Published: October 2022

Abstract
Purpose
Patients with Gastrointestinal (GI) malignancies more frequently present with anemia. A large proportion of them exhibits a systemic inflammatory response causing anemia of chronic disease. Although tumor factors play an essential role in determining the prognosis, studies have also reported the role of host factors in the outcome. This study was done to determine the association between systemic inflammation, tumor characteristics, and immediate postoperative outcome among various anemia subgroups.
Methods
This was a single-center, prospective observational study of patients undergoing treatment for GI cancers. The modified Glasgow prognostic Score (mGPS) was calculated and patients were followed up until discharge regarding postoperative complications, length of the hospital, ICU stay, and mortality. The patient demographics, tumor characteristics, systemic inflammation, and postoperative complications were studied among different subtypes of anemia.
Results
A total of 209 patients were analysed, and a higher prevalence of normocytic anemia (52.63%) and systemic inflammatory response (62.21%) was observed among the study population. Systemic inflammation was significantly associated with a higher nodal stage (p = 0.02) and margin positivity (p = 0.006). Patients with higher mGPS had significantly higher requirements for blood transfusion (p = 0.003), superficial SSI (p = 0.03), and sepsis (p = 0.02), however, other postoperative complications were comparable among patients without an inflammatory response.
Conclusion
High prevalence of systemic inflammation and resultant normocytic anemia among GI cancers warrants thorough knowledge in this field among the treating surgeons. This study opens up a platform for further research to consider anti-inflammatory agents in its management to circumvent the morbidity of blood transfusions.
Distributed Multi-robot Collaboration for Tasks Allocation based on Optimized Greedy Algorithm Approach
Authors: TAMALI Abderrahmane , AMARDJIA Nourredine , TAMALI Mohammed 
Published: October 2022

Abstract
During the last decades, the intervention using robots in sensitive areas reached appreciable mathematical confidence. Robots are equipped with adequate payload and embed processes using high-performance algorithms oriented topology, statistical observations, ontology, or bio-inspired. These algorithms improve considerably the processing capacity in time savings and computational efficiency.The modified GREEDY approach adopted in this contribution aims to optimize the gain in time and cost of processing for task allocation among a cluster of micro-robots with adequate means for the purpose of identifying sensitive areas.Evaluation of the efficiency of the task’s planning process to order each agent micro-robot, we optimally evaluate the cost function by grouping the dependencies; radio connectivity, energy at disposal, and the absolute and relative availability of the agent for itself and within the group. One of the first concerns is to validate the positive trend of the growing number of agents forming the cluster.For this objective, our approach introduces a cluster of three micro-robots. The proposed idea is qualified as an adaptive approach for a mission to identify victims at risk in a challenging environment. Each micro-robot in the cluster is configured to maintain interoperability and collaboration that gain support to evolve in the target scene in order to perform the assigned task. Collaboration algorithms are implemented as an adaptive strategy where it is necessary to optimize agents' mobility according to criteria depending on the characteristic of the place to be identified.
Synthesis of lignite-based Ni/C composite with low-medium temperature pyrolysis method as an efficient Pt-free counter electrode for dye-sensitized solar cells
Authors: Mao Wei , Wei Liguo , Zhao Lishuang , Xu Xiaohu , Lin Jianmin , He Huiyi , Zhang Xindan , Zhang Zihan 
Published: October 2022

Abstract
In order to obtain inexpensive Pt-free counter electrode materials for dye sensitized solar cells and expand the application fields of low-rank coal, lignite-based Ni/C composite counter electrode materials were prepared by low-medium temperature pyrolysis method using Huolinhe Lignite as raw material. The structure and chemical components of as-synthesized lignite-based Ni/C composite counter electrode materials were characterized by XRD, FT-IR, TG, Raman, SEM, TEM and XPS, and the electrocatalytic activity of lignite-based Ni/C composite counter electrode were investigated by cyclic voltammetric curve, electrochemical impedance spectrum, Tafel polarization curve. It is found that the electrocatalytic activity of the lignite was improved after low-medium temperature pyrolysis and composited with Ni species to form lignite-based Ni/C composites, which helps to catalyze the electrolyte reduction and thus improve the photoelectric conversion efficiency of the cell. The photoelectric conversion efficiency (η) of the lignite-based Ni/C composite counter electrode was 3.42% (Jsc = 11.49 mA cm-2, Voc = 0.75 V, FF = 0.40) significantly higher than that of the lignite counter electrode (η = 0.20%, Jsc = 3.16 mA cm-2, Voc = 0.72 V, FF = 0.09). This indicates that low-medium temperature pyrolysis and composition with Ni is an effective method to improve the photovoltaic performance of coal-based counter electrode materials.
Nano cellulose packaging for increasing the shelf life of food Products
Authors: Cheran E , Sharmila C , Lakshmanan A , Subramanian P , Raja K , Divyabharathi P 
Published: October 2022

Abstract
Studies were carried out to develop eco-friendly Packaging material for extended shelf-life of food products. Nano cellulose (NC) from Maize Cob was used as filler in the synthesis of Nanopolymers enriched with Thyme oil, Cinnamon oil, clove oil, and Rosemary oil. Characterization of nanopolymer coated bag and their effect on enhancing the shelf-life of food products in different temperature condition was also studied. Nanocellulose was extracted from the maize cob using steam coupled chemical treatment. The average size of NC extracted from the maize cob was found to be around 20–100 nm when observed under Scanning Electron Micrscope (SEM) and Transmission Electron Microscope (TEM). The polymer based nano-films were developed using NC (1%), Poly Lactic Acid (PLA) (7%), Glycerol Triacetate (3%) and the nano polymers were enriched separately with thyme oil, Cinnamon oil, Clove oil and Rosemary oil at a concentration of 5000 ppm. The functional characterization of the nanocomposites was done using Fourier Transform Infrared Spectroscopy (FT-IR) and the developed nanocomposite coated sheet showed that the peaks corresponding to the aromatic groups were obtained at 2981cm− 1. The peaks of the cellulose were present at 1215 cm− 1. The Triacetin compounds were present at the range of 1063 cm− 1. The X-Ray Diffraction (XRD) pattern of the Maize cob, Cellulose and Nanocellulose exhibited strong peaks at 2θ ≈ 22.44°, 22.49°, and 22.03° respectively. The particle size of 213.5nm and zeta of -34mV was observed. TGA curve has shown that all the nanocomposites films got degraded at a temperature of 400°C. Approximately 95% of the samples degraded thermally above 360°C.The contact angle of uncoated paper sheet was found to be hydrophilic in nature with the angle of 78o and the different essential oil based nanocomposite coated sheet of thyme, cinnamon, clove and rosemary exhibited the angle of 115.6°, 100.1°, 95.1° and 88.9° which are hydrophobic in nature. The insect repellency test was carried out for nanocomposite coated and uncoated bags and the result show that nanocomposite bags repel the insects with the efficiency of more than 80% and the uncoated bags with efficiency of 20%. The antifungal test was carried out for different essential oil based nanocomposites against Fusarium spp and the nanocomposites show good antifungal property with 100% inhibition zone.
Sleep and sleep hygiene of adolescents with and without ADHD during COVID-19
Authors: Marten Finja , Keuppens Lena , Baeyens Dieter , Boyer Bianca , Danckaerts Marina , Oord Saskia Van der 
Published: October 2022

Abstract
Restrictions related to the COVID-19 pandemic may have significantly impacted adolescents’ sleep, possibly even more so in adolescents with attention-deficit/hyperactivity disorder (ADHD). The current study examines the sleep of adolescents without ADHD (TD) and a homogeneous group of adolescents with ADHD and comorbid sleep problems using a multi-method objective and subjective assessment of sleep outcomes, during few and multiple COVID-19 restrictions. Four groups (2 TD and 2 ADHD) of in total 100 adolescents (50 TD and 50 ADHD) were included. One ADHD group was tested during numerous COVID-19 restrictions, the other during few. The same was done with the two TD groups. All participants were between 13 and 17 years old. Three two-way MANCOVAs were implemented with ADHD diagnosis and level of COVID-19 restrictions as independent and sleep outcomes (sleep hygiene, sleep deprivation, and objective and subjective total sleep time (TST) and sleep onset latency (SOL)) as dependent variables. As expected, adolescents with ADHD and comorbid sleep problems had significantly more subjective and objective sleep problems. Additionally, COVID-19 restrictions were related to sleep outcomes in both TD and ADHD groups, with both objective TST and subjective SOL being shorter when there were numerous COVID-19 restrictions. However, the shorter SOL was only found in the ADHD group. COVID-19 restrictions are related to sleep in adolescents, and more so in the ADHD group. Future research should focus on mechanisms underlying these relations.
Identification of novel a chromatin regulator signature predicting overall survival for breast cancer
Authors: Liu Jingting , Zhou Yawei , Zhou Qingyun , Wang Wendi , Wang Yanxia , Zhao Peng 
Published: October 2022

Abstract
Background
Breast cancer (BRCA) has the highest incidence among female malignancies. Increasing evidence has confirmed that chromatin regulators (CRs) are involved in cancer progression. We aim to develop a chromatin regulators signature to predict the prognosis of BRCA patients.
Methods
The mRNA expression profiles and corresponding clinical data of BRCA patients were downloaded from TCGA databases, and then the differentially expressed CRs genes were screened. We also used the least absolute shrinkage and selection operator (LASSO) Cox regression model to construct a multigene signature with CRs genes of BRCA patients and evaluated the efficacy of the CRs prognostic signature with GEO validation cohort. Then, we explored the differences of tumor immune cell infiltration, tumor mutation burden (TMB), and patients’ response to chemotherapy between the different risk group.
Results
This CRs prognostic signature consisted of 8 chromatin regulators related genes, which was an independent prognostic factor of BRCA. The prognostic signature was further validated in GSE42568 cohort. According to the signature, patients with BRCA were divide into two risk groups. Functional annotation and the pathway analysis showed that the high-risk group had an enrichment of many tumor features, including cGMP-PKG signaling pathway, chromatin remodeling, DNA repair, thyroid cancer, cell cycle, homologous recombination, lysine degradation, pathways in cancer. Moreover, the immune cell types of tumor immune microenvironment and the TMB value of patients in the high-risk group was significantly different from those in low-risk group. ROC curves and nomogram indicated that the risk score had a good accuracy for predicting the survival of BRCA patients.
Conclusions
We identified a novel CRs prognostic signature which could precisely predict the prognosis of breast cancer patients. Our work elucidated that the CRs genes may serve as an indispensable player in complexity and diversity of tumor progression.
Elevated expression of circulating CDR1as in childhood dilated cardiomyopathy patients; Expanding MAPK signaling pathway role in dilated cardiomyopathies pathogenesis.
Authors: Esmaiel Nora N. , Ibrahim LA. , Fayez Alaaeldin , Ammar TH , El-Bassyouni Hala T. 
Published: October 2022

AbstractBackground Circular RNAs (circRNAs) are non-coding RNAs resulting from back splicing of pre-mRNA. circRNAs exhibit higher stability with multiple functional modes. Thus, this circRNAs characteristic make it a promising entity for the development of diagnostic tools and therapies for human disease. In the present study, we mainly aimed to evaluate two circRNAs CDR1as and has-circRNA_105039 as noninvasive biomarkers for childhood dilated cardiomyopathy and ventricular septal defects patients. Methods Fold change of CDR1as and has-circRNA_105039 was detected by qRT-PCR in 101 participants. The diagnostic accuracy of CDR1as was determined by receiver operating characteristic (ROC) analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEEG) pathway analyses were performed to predict CDR1as/miRNAs and CDR1as/proteins interaction networks related to congenital ventricular septal defects (VSD) and childhood dilated cardiomyopathy (DCM). Results CDR1as showed significant higher fold change (FC = 2.8) in DCM group than controls and VSD groups. Experimental evidence-based GO and KEGG pathways analysis showed that has-miR-7-5p and hsa-miR-619 targeted 3'UTR of three mRNAs involved in MAPK signaling pathway. Conclusion Upregulated CDR1as may influence the level of has-miR-7-5p and hsa-miR-619 in childhood DCM patients, and further relieve the inhibitory effect of these miRNAs on kinases of the MAPK signaling pathway.
Revisiting the Pathology of Bilateral Macronodular Adrenocortical Disease in Light of Recent Discoveries
Authors: Botelho Maria Luiza Anhaia Arruda , Nishi Mirian Yumie , Zerbini Maria Claudia Nogueira 
Published: October 2022

Abstract
Bilateral Macronodular Adrenocortical Disease (BMAD) is a rare cause of ACTH-pituitary-independent Cushing’s syndrome. The recent discovery of a relationship with pathogenic variants of ARMC5, a putative tumor suppressor gene, suggests that genetic determination of the disease is more common than previously thought. In view of apparently sporadic and familial cases from a single tertiary center, we carried out a detailed morphologic review of 22 surgical specimens excised from patients with BMAD, comparing those with pathogenic variants of ARMC5 (PV+, n = 14) and those without (PV−, n = 8), as well as comparing those groups with a control group (n = 11). We also evaluated the expression of Ki-67, BCL-2, BAX, p53, ACTH, and ARMC5 protein. We observed that capsular extrusion, and trabecular and pseudoglandular architectural patterns, were more common in the PV+ group than in the PV− group, suggesting that the boundaries between hyperplastic and neoplastic processes are unclear in BMAD. The disorganized adrenal growth in BMAD was found to be unrelated to increased cell proliferation evaluated by Ki-67 or to changes in the expression of apoptosis-regulating proteins (BCL2 and BAX). In all cases, the ACTH immunoexpression was observed only in the adrenal medulla, suggesting that disruption of the corticomedullary architecture due to large confluent cortical nodules causes an imbalance in ACTH regulation in this intricate morphofunctional relationship. These data open new possibilities for comprehension of this heterogeneous adrenal disorder and further studies can clarify the role of cross-talking of the ACTH in BMAD nodules.
Exosome-derived lncRNA HOXA-AS3 promotes castration resistance and progression of prostate cancer via the miR-29b-3p/Mcl-1/STAT3 axis
Authors: Teng Jie , Zhao Yan , Shang Limin , Li Yang , Zhang Jian , Zhu Liang , Li Gang , Liu Zhifei , Jia Mingfei , Kang Shaosan , Chen Yegang , Niu Yuanjie , Cai Qiliang 
Published: October 2022

Abstract
Background
Tumor microenvironment are involved in the progression of prostate cancer as an "accomplice", and cancer cell–secreted exosomes were identified as crucial messengers can carry lncRNAs to participate in intercellular communication. Herein, we report that an lncRNA HOXA-AS3, was correlated positively with the castration resistance and progression of prostate cancer.
Methods
Indirect co-culture of PC-3 and LNCaP was performed to explore the androgen resistance of prostate cancer. MTT, colony formation test, exosome isolation, identification and uptake test, and western blot confirmed that exosomes promote androgen resistance in LNCaP cells. Further, the molecular mechanism of HOXA-AS3 was proved by bioinformation analysis, Dual-Luciferase Reporter Gene Assays, gene knockout and overexpression experiment, cell proliferation and apoptosis experiment, qPCR, immunofluorescence experiment and rescue experiment. Finally, rats xenografts and prostate tissue section were used to examine the role of HOXA-AS3 in the transformation of ADPC into CRPC.
Results
Exosome-derived lncRNA HOXA-AS3 promotes the proliferation, migration, invasion and hormone resistance of prostate cancer cells. HOXA-AS3 functions as a competing endogenous RNAs (ceRNA) for miRNA-29b-3p, thereby elevating Mcl-1 and STAT3 expression, inhibiting the release of cytochrome c and the activation of Caspases-9, resulting in the decrease of hormone sensitivity, and finally leads to androgen resistance and progression of prostate cancer.
Conclusions
lncRNA HOXA-AS3 participates in the castration resistance and progression of prostate cancer through regulating the miR-29b-3p/Mcl-1/STAT3 pathway. lncRNA HOXA-AS3 might have the potential to serve as a candidate intervention targets for prostate cancer treatment.
Current treatment strategies for postoperative intrahepatic bile duct stones in congenital biliary dilatation: A single center retrospective study
Authors: Takimoto Atsuro , Fumino Shigehisa , Iguchi Masafumi , Takemoto Masakazu , Takayama Shohei , Kim Kiyokazu , Higashi Mayumi , Aoi Shigeyoshi 
Published: October 2022

Abstract
Background: Intrahepatic bile duct (IHBD) stones are one of the most common late complications of Roux-en-Y hepaticojejunostomy for congenital biliary dilatation (CBD). We report the current treatment strategies for IHBD stones and their outcomes in our institute.
Methods: Between 1983 and 2021, 117 patients with CBD were surgically treated in our institute. Our treatment strategies included oral ursodeoxycholic acid (UDCA), double-balloon endoscopic retrograde cholangiography (DB-ERC), percutaneous cholangio-drainage (PTCD), and open surgery. A retrospective study was conducted using medical charts.
Results: Postoperative IHBD stones were identified in 12 of 117 patients with CBD (10.2%). Five patients received UDCA, and small stones were successfully resolved in two cases. DB-ERC was performed eight times in five patients, but the endoscope could not reach the porta hepatis due to a long jejunal loop in two of five patients. One patient presented with severe acute pancreatitis induced by prolonged DB-ERC. PTCD was performed in three patients, two of whom finally underwent open surgery due to unsuccessful lithotomy. Open surgery was eventually performed in three patients. Lithotomy was performed in one patient; lithotomy with strictureplasty was performed in another patient. The other patient was diagnosed with intrahepatic cholelithiasis with adenocarcinoma. He underwent left lobectomy and died of carcinomatous peritonitis.
Conclusions: Oral UDCA may be effective for small stones. Although DB-ERC should be considered as a first-line interventional therapy for lithotomy, it may not be feasible due to a long jejunal loop, and pancreatitis may occur. Long-term follow-up and early detection and treatment for IHBD stones may yield a good prognosis.
Effects of acidic phosphorus-rich biochar from halophyte species on P availability and fractions in alkaline soils
Authors: Wang Xuyang , Sun Tao , Ma Haigang , Tang Guangmu , Chen Mo , Abulaizi Maidinuer , Yu Guangling , Jia Hongtao 
Published: October 2022

Abstract
Conditioning alkaline soil with acidic phosphorus-rich biochar might contribute to achieving sustainable phosphorus (P) use and residue management. In this study, acidic phosphorus-rich biochar was prepared from halophyte species biochar (HBC) by the modification of H3PO4 (P-HBC) and H4P2O7 (PA-HBC). The P combination method and fractions in biochars were characterized, and the effects of biochars on P fractions in different types of alkaline soil were examined with incubation experiments. The results showed that the pH values of P-HBC (3.31) and PA-HBC (2.17) decreased significantly, and the total P content increased to 4.66 g·kg− 1 and 5.24 g·kg− 1, respectively. The spectral analysis confirmed the doping of P and acid groups in biochar. The addition of modified biochars in alkaline soils will makes the soil tend to be neutral. Sequential extraction of soil P confirmed that biochars application facilitated the transformation of stable P into active fractions, with greater effects from modified biochars than HBC. Activation mechanism of biochar to soil P fractions varies from soil types. Overall, acidic phosphorus-rich biochars can be employed to improve alkaline soil properties and increase P activity.