bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potentials of miR-9-5p in promoting epileptic seizure and improving survival of glioma patients
Authors: Wang Shenglin , He Xuzhi , Bao Nana , Chen Mingyue , Ding Xiaomi , Zhang Ming , Zhao Li , Wang Shunxian , Jiang Guohui 
Published: November 2022

Abstract
Background
Epilepsy affects over 70 million people worldwide; however, the underlying mechanisms remain unclear. MicroRNAs (miRNAs) have essential functions in epilepsy. miRNA-9, a brain-specific/enriched miRNA, plays a role in various nervous system diseases and tumors, but whether miRNA-9 is involved in epilepsy and glioma-associated epilepsy remains unknown. Therefore, we aimed to explore the potential role of miR-9-5p in seizures and its effect on the survival of glioma patients, in order to provide new targets for the treatment of epilepsy and glioma.

Methods
The YM500v2 database was used to validate the expression of hsa-miR-9-5p in tissues. Moreover, qRT-PCR was performed to investigate the expression of miR-9-5p in temporal lobe epilepsy patients and rats with lithium-pilocarpine-induced seizures. Recombinant adeno-associated virus containing miR-9-5p was constructed to overexpress miR-9-5p in vivo. The effects of miR-9-5p on the behavior and electroencephalographic activities of the lithium-pilocarpine rat model of epilepsy were tested. Bioinformatics analysis was used to predict the targets of miR-9-5p and explore its potential role in epilepsy and glioma-associated epilepsy.

Results
The expression of miR-9-5p increased at 6 h and 7 days after lithium-pilocarpine-induced seizures in rats. Overexpression of miR-9-5p significantly shortened the latency of seizures and increased seizure intensity at 10 min and 20 min after administration of pilocarpine (P < 0.05). Predicted targets of miR-9-5p were abundant and enriched in the brain, and affected various pathways related to epilepsy and tumor. Survival analysis revealed that overexpression of miR-9-5p significantly improved the survival of patients from with low-grade gliomas and glioblastomas. The involvement of miR-9-5p in the glioma-associated epileptic seizures and the improvement of glioma survival may be related to multiple pathways, including the Rho GTPases and hub genes included SH3PXD2B, ARF6, and ANK2.

Conclusions
miR-9-5p may play a key role in promoting epileptic seizures and improving glioma survival, probably through multiple pathways, including GTPases of the Rho family and hub genes including SH3PXD2B, ARF6 and ANK2. Understanding the roles of miR-9-5p in epilepsy and glioma and the underlying mechanisms may provide a theoretical basis for the diagnosis and treatment of patients with epilepsy and glioma.
Intramedullary rod insertion places the femoral component more laterally during Oxford medial unicompartmental knee arthroplasty
Authors: Tanaka Toshikazu , Suda Yoshihito , Kamenaga Tomoyuki , Saito Akira , Fujishiro Takaaki , Okamoto Koji , Hiranaka Takafumi 
Published: November 2022

Abstract
Background
This study aims to assess the influence of intramedullary rods on the implantation positions of femoral components using Microplasty instrumentation in Oxford unicompartmental knee arthroplasty. We hypothesized that femoral components can be laterally implanted incorrectly when using intramedullary rods.

Methods
This prospective study included all 45 consecutive patients (53 knees) who underwent Oxford unicompartmental knee arthroplasty surgery for anteromedial osteoarthritis or spontaneous osteonecrosis of the knee at our hospital during the study period. A custom-made toolset comprising a triangular caliper and circular trial bearings was used to evaluate the distance between the bearing and the vertical wall of the tibia implant (wall-bearing space) using the caliper at 90° flexion both with and without intramedullary rods.

Results
The wall-bearing space was significantly larger when the intramedullary rod was used than when intramedullary rod was not used (1.8 ± 1.1 mm versus 3.4 ± 1.2 mm, P < 0.001). The mean difference of wall-bearing space with and without intramedullary rod was 1.6 ± 0.7 mm.

Conclusions
Femoral components can be laterally implanted incorrectly by an average of 1.6 mm when using intramedullary rods. The wall-bearing space should be evaluated using trial components, and if the relationship is improper, it should be corrected before keel slot preparation.
Hypothyroidism Prevalence in the United States: A Retrospective Study Combining National Health and Nutrition Examination Survey and Claims Data, 2009–2019
Authors: Wyne Kathleen L , Nair Lekshmi , Schneiderman Chris P , Pinsky Brett , Antunez Flores Oscar , Guo Dianlin , Barger Bruce , Tessnow Alexander H 
Published: November 2022

Abstract
Previous estimates determined prevalence of hypothyroidism (HT) to be 4.6% of the US population. This study aimed to update estimates of HT prevalence in the United States by retrospective analysis of 2 datasets. Data on HT type (overt or subclinical HT) and treatment were collected from the 2009-2010 and 2011-2012 National Health and Nutrition Examination Survey (NHANES) cycles. From the Optum administrative claims database, medical and pharmacy claims were collected between January 1, 2012, and December 31, 2019. Patients were defined as having HT if, per given year, they had &gt;1 prescription for HT treatment, &gt;1 claim indicating an HT diagnosis, or thyroid-stimulating hormone levels &gt;4.0 mIU/L (NHANES arm). For both studies, treatment was defined as any evidence of synthetic or natural thyroid hormone replacement, identified by pharmacy claims or patient surveys. Data are reported as percentage of patients with HT and treatments received. Between 2009 and 2012, HT prevalence remained around 9.6% of the US population. The administrative claims dataset showed that HT prevalence grew from 9.5% in 2012 to 11.7% in 2019 and that &gt;78% of patients received thyroxine (T4) monotherapy. Similarly, the NHANES dataset showed that T4 replacement therapy was the most common treatment for HT. From 2012–2019, patients with untreated HT grew from 11.8% to 14.4%. The prevalence of HT in the United States has steadily increased since 2009. Likewise, the percentage of hypothyroid-diagnosed patients not receiving treatment also increased, suggesting that the increased prevalence may be due to increased cases of subclinical HT.
Obstructive Sleep Apnea Among Gravidas With Chronic Hypertension Compared to Matched Controls: A Prospective Cohort Study
Authors: Dominguez Jennifer E. , Grotegut Chad A. , Wright Mary Cooter , Habib Ashraf S. 
Published: November 2022

BACKGROUND:
Previous studies of obstructive sleep apnea (OSA) risk in gravidas with chronic hypertension (cHTN) did not control for obesity as a risk factor for OSA. We therefore performed this study to evaluate whether OSA is more prevalent among gravidas with cHTN compared to normotensive gravidas matched for body mass index (BMI) and gestational age (primary outcome). We also assessed whether OSA is more severe when comorbid with cHTN in pregnancy (secondary outcome).

METHODS:
This was a single-center, prospective cohort study. Adult gravidas at 10–20 weeks of gestation, with and without cHTN, were enrolled and BMI matched. All subjects answered OSA screening questionnaires and underwent a home sleep test when they were between 10 and 20 weeks of gestation. Pregnancy outcomes were followed for all subjects. We performed univariable and multivariable logistic regression to model the relationship between cHTN status and OSA.

RESULTS:
A total of 100 pregnant subjects (50 with cHTN and 50 normotensive) completed a home sleep test of 2 hours or more. There were no differences in demographic variables between the 2 groups, except that gravidas with cHTN were significantly older than normotensive subjects (mean ± standard deviation [SD] 34 ± 4 vs 30 ± 6 years; P < .001). OSA was more prevalent (64% vs 38%; P = .009; odds ratio [95% confidence interval (CI)] 2.90 [1.30–6.65]; P = .01) and more severe in gravidas with cHTN (moderate or severe OSA 59% vs 21%; P = .009). After controlling for age, we found no overall association between cHTN on OSA risk (adjusted odds ratio [95% CI] 2.22 [0.92–5.40]; P = .076). However, among gravidas older than 25 years of age, cHTN was associated with higher odds of OSA (adjusted odds ratio [95% CI], 2.64 [1.06–6.71], P = .038).

CONCLUSIONS:
cHTN and age are important risk factors for OSA in gravidas. Gravidas with cHTN should be screened for OSA in early pregnancy. Future studies may validate screening tools that include cHTN and age, and investigate the role of OSA therapy in blood pressure control.
Proactive Grief
Authors: Ghanayem Eman 
Published: November 2022

Abstract
Part memoir, part theoretical reflection, this essay offers one answer to the question “How do Palestinians grieve?” In this narration of the author’s mother’s relationship to death, her multiple displacements, and her plan for her life, the term proactive grief is used to theorize how and why her mother’s life trajectory was shaped by her strife to have a dignified death, in other words, to be able to die in Palestine. To illuminate the significance of her mother’s approach to death and its relationship to being Palestinian, being refugee, and living under colonial war conditions, this essay also reflects on the difficulty of writing about grief while being personally entangled in its complicated emotions. Ultimately, her mother’s proactiveness and commitment to home within and beyond life present an intimate narrative and a family history that could show readers what it means to be Palestinian, to live colonization, to love home, and to face constant threat with dignity.
PTP10D-mediated cell competition is not obligately required for elimination of polarity-deficient clones
Authors: Gerlach Stephan U. , de Vreede Geert , Bilder David 
Published: November 2022

ABSTRACT
Animal organs maintain tissue integrity and ensure removal of aberrant cells through several types of surveillance mechanisms. One prominent example is the elimination of polarity-deficient mutant cells within developing Drosophila imaginal discs. This has been proposed to require heterotypic cell competition dependent on the receptor tyrosine phosphatase PTP10D within the mutant cells. We report here experiments to test this requirement in various contexts and find that PTP10D is not obligately required for the removal of scribble (scrib) mutant and similar polarity-deficient cells. Our experiments used identical stocks with which another group can detect the PTP10D requirement, and our results do not vary under several husbandry conditions including high and low protein food diets. Although we are unable to identify the source of the discrepant results, we suggest that the role of PTP10D in polarity-deficient cell elimination may not be absolute.
A new software tool for computer assisted in vivo high-content analysis of transplanted fluorescent cells in intact zebrafish larvae
Authors: Førde Jan-Lukas , Reiten Ingeborg Nerbø , Fladmark Kari Espolin , Kittang Astrid Olsnes , Herfindal Lars 
Published: November 2022

ABSTRACT
Acute myeloid leukemia and myelodysplastic syndromes are cancers of the bone marrow with poor prognosis in frail and older patients. To investigate cancer pathophysiology and therapies, confocal imaging of fluorescent cancer cells and their response to treatments in zebrafish larvae yields valuable information. While zebrafish larvae are well suited for confocal imaging, the lack of efficient processing of large datasets remains a severe bottleneck. To alleviate this problem, we present a software tool that segments cells from confocal images and track characteristics such as volume, location in the larva and fluorescent intensity on a single-cell basis. Using this software tool, we were able to characterise the responses of the cancer cell lines Molm-13 and MDS-L to established treatments. By utilizing the computer-assisted processing of confocal images as presented here, more information can be obtained while being less time-consuming and reducing the demand of manual data handling, when compared to a manual approach, thereby accelerating the pursuit of novel anti-cancer treatments. The presented software tool is available as an ImageJ java-plugin at https://zenodo.org/10.5281/zenodo.7383160 and the source code at https://github.com/Jfo004/ConfocalCellSegmentation.
Ψευδείς ειδήσεις και περιβαλλοντική επικοινωνία
Authors: Λάμπρου Ευάγγελος , Αντωνόπουλος Νίκος 
Published: November 2022

Η έννοια της «περιβαλλοντικής επικοινωνίας» αποτελεί τον ακρογωνιαίο λίθο της ικανότητας της επιστήμης της βιωσιμότητας να επιτύχει τους στόχους της και ιδιαίτερα να δημιουργήσει καλύτερες προϋποθέσεις για τη σύνδεση της γνώσης με τη δράση και την ουσιαστική εφαρμογή των αρχών της. Η ικανότητα της περιβαλλοντικής επικοινωνίας να δημιουργεί τις συνθήκες εκείνες της ενημέρωσης και της πληροφόρησης του ευρύτερου κοινού δίνουν τη δυνατότητα στην επιστήμη της βιωσιμότητας να κάνει γνωστούς τους στόχους της και να καταστεί σχετική με τις ανάγκες των ενδιαφερομένων και των κοινωνικών εταίρων. Ωστόσο, σε πολλές περιπτώσεις η κοινή γνώμη πέφτει θύμα παραπληροφόρησης με τις ψευδείς ειδήσεις να αποτελούν σημαντική τροχοπέδη για τη σωστή επικοινωνία και την διαμόρφωση της «πράσινης συνείδησης» στην κοινωνία (Lindenfeld et al., 2012).Πολλές φορές λοιπόν, τα περιβαλλοντικά θέματα δεν έχουν τελικά την απήχηση που θα έπρεπε να έχουν αποκτήσει στην κοινή γνώμη λόγω της κακής ή ανεπαρκούς επικοινωνίας των χαρακτηριστικών τους ή και ακόμα λόγω της παραπληροφόρησης και των ψευδών ειδήσεων που διακινούνται διαμέσου διάφορων διαύλων και μέσω των Μέσων Κοινωνικής Δικτύωσης με αποτέλεσμα οι πολίτες να αποκτούν λανθασμένη εικόνα ή και πληροφόρηση για πολλά κρίσιμα ζητήματα που αφορούν στο περιβάλλον και την βιωσιμότητά του. Η συγκεκριμένη έρευνα κάνει μια προσπάθεια να καταδείξει ότι η ορθή επικοινωνία των περιβαλλοντικών θεμάτων και ζητημάτων απειλείται και από την ύπαρξη του φαινομένου των ψευδών ειδήσεων οι οποίες πολλές φορές εμφανίζονται ως ψευδοεπιστημονική γνώση ή ως εναλλακτική ειδησεογραφία. Μέσω τις κατάρριψης ψευδών ειδήσεων από την ελληνική πλατφόρμα “Ellinika Hoaxes” καταδεικνύεται η συνεχής παρουσία παραπληροφόρησης πάνω σε ζητήματα που αφορούν την ενημέρωση για το περιβάλλον αλλά και οι κίνδυνοι που προκύπτουν από αυτήν για την κοινωνία και την προστασία των περιβαλλοντικών πόρων εν γένει.
Απτικά ψηφιακά παιχνίδια
Authors: Κίργινας Σωτήρης 
Published: November 2022

Η παιγνιακή εμπειρία που βιώνεται κατά τη διάρκεια της αλληλεπίδρασης παίκτη παιχνιδιού παραδοσιακά εξαρτάται από την οπτική και την ηχητική ανατροφοδότηση για την παροχή πληροφοριών στους παίκτες. Άλλοι αισθητηριακοί τρόποι πουπροκύπτουν από την απτική αλληλεπίδραση ανθρώπου-μηχανής, όπως επί παραδείγματι ανατροφοδότηση μέσω δονήσεων και παλμικών κινήσεων, θερμική ανατροφοδότηση, ανατροφοδότηση μέσω άσκησης δυνάμεων και μέσω παραμορφώσεων, επαυξάνουν και εμπλουτίζουν την ποιότητα της αλληλεπίδρασης παίκτη - παιχνιδιού. Στο πλαίσιο αυτό, σκοπός της παρούσας εργασίας είναι η διερεύνηση των τρόπων με τους οποίους τα χαρακτηριστικά των απτικών ψηφιακών παιχνιδιών επηρεάζουν και βελτιώνουν την επικοινωνία ανθρώπου-μηχανής και κατά συνέπεια, δημιουργούν θετικότερη παιγνιακή εμπειρία, μεγαλύτερη αίσθηση εμβύθισης και παρουσίας σε σύγκριση με τα μη απτικά, συμβατικά ψηφιακά παιχνίδια, αποτελώντας σημαντικά εργαλεία που μπορούν να αξιοποιηθούν με μεγάλη επιτυχία στην περιβαλλοντική επικοινωνία. Η εργασία ολοκληρώνεται με προτάσεις αξιοποίησης διαφορετικών απτικών ανατροφοδοτήσεων για την επίτευξη ακόμη μεγαλύτερης βελτίωσης της παιγνιακής εμπειρίας και της περιβαλλοντικής επικοινωνίας.
Ψηφιακός πολιτισμός και περιβαλλοντική επικοινωνία
Authors: Κιουρεξίδου Ματίνα 
Published: November 2022

Οι φιλικές προς το περιβάλλον ιστοσελίδες είναι μια νέα προσέγγιση που βάζει τους ανθρώπους και τον πλανήτη σε πρωταρχικό ρόλο. Κάθε μέρα οι δραστηριότητες που πραγματοποιούνται ψηφιακά αυξάνονται. Μπορεί το διαδίκτυο να είναι άυλο αλλά καταναλώνει μεγάλες ποσότητες σε ενέργεια για να λειτουργήσει. Οι ανανεώσιμες πηγές ενέργειας, η χρήση του μαύρου χρώματος, η μείωση του μεγέθους και η μείωση CO2 μιας ιστοσελίδας είναι μερικοί από τους ευκολότερους και φθηνότερους τρόπους για να συμβάλλουν θετικά στα κλιματικά ζητήματα τα μουσεία και όχι μόνο.
Risk factors common to leading eye health conditions and major non-communicable diseases: a rapid review and commentary
Authors: Keay Lisa , Ren Kerrie , Nguyen Helen , Vajdic Claire , Odutola Michael , Gyawali Rajendra , Toomey Melinda , Peters Ruth , Ee Nicole , Dillon Lisa , Hackett Maree , Ah Tong Brandon , D'Esposito Fabrizio , Faulmann David , Burton Matthew , Ramke Jacqueline , Jalbert Isabelle 
Published: November 2022

Background: To gain an understanding of the intersection of risk factors between the most prevalent eye health conditions that are associated with vision impairment and non-communicable diseases (NCDs). Methods: A series of rapid reviews of reviews reporting on non-modifiable risk factors, age and sex, and modifiable risk factors, including social determinants, were conducted for five common eye health conditions that are the leading causes of vision impairment globally (refractive error including uncorrected refractive error, cataract, age-related macular degeneration (AMD), glaucoma, and diabetic retinopathy) and five prevalent NCDs (cancer, cardiovascular disease, chronic respiratory disease, dementia, and depressive disorders). Articles published within approximately 5 years to the end of 2019 were identified through expert recommendation, PubMED, Ovid Medline, the Lancet Global Burden of Disease series, the International Agency for Research on Cancer and World Cancer Research Fund. Results: Of 9,213 records identified, 320 records were eligible. Eye health conditions and NCDs share many risk factors. Increased age was found to be the most common shared risk factor, associated with increased risks of AMD, cataract, diabetic retinopathy, glaucoma, refractive error, cancer, cardiovascular disease, chronic obstructive pulmonary disease, and dementia. Other shared risk factors included smoking, obesity, alcohol consumption (mixed results), and physical activity was protective, though limited evidence was found for eye conditions. Social determinants are well documented as risk factors for NCDs. Conclusion: There is substantial overlap in common established risk factors for the most frequent vision impairing eye conditions and leading NCDs. Increasing efforts should be made to integrate preventative and risk reduction interventions to improve health, with greatest shared benefits for initiatives which aim to reduce smoking, improve diet, and promote physical activity.
The role of IL-6, ferritin, and coagulopathy in COVID-19 clinical progression
Authors: Harahap Alvin Tagor , Irawan Cosphiadi , Susilo Adityo , Harimurti Kuntjoro , Gathmyr Dewi , Shatri Hamzah , Lubis Anna Mira , Nainggolan Leonard , Abdullah Murdani 
Published: November 2022

Background In COVID-19, the release of pro-inflammatory mediators in the cytokine storm, primarily interleukin-6 (IL-6), has been hypothesized to induce pulmonary intravascular coagulation. However, the relationship between IL-6 and coagulopathy remains unclear in COVID-19 progression. We aimed to investigate the correlation of IL-6 with D-dimer, fibrinogen, prothrombin time (PT), and ferritin. Furthermore, we also analyzed the effect of those parameters on the worsening of COVID-19 patients. Methods A prospective cohort study was conducted in moderate and severe COVID-19 patients from June 2020 to January 2021. A serial evaluation of IL-6, D-dimer, fibrinogen, ferritin, and PT was performed and correlated with the patient's condition at admission and on the 14th day. The outcomes (improvement, worsening, or discharged patients) were recorded during the study. Results Of 374 patients, 73 study subjects (61 severe and 12 moderate COVID-19) were included in this study. A total of 35 out of 61 severe and one out of 12 moderate illness subjects had experienced worsening. Spearman-rank correlation of IL-6 with with ferritin, D-dimer, fibrinogen, and PT was 0.08 (p=0.5), −0.13 (p=0.27), 0.01 (p=0.91), and 0.03 (p=0.77), respectively. In ROC analysis, D-dimer (74,77%) and IL-6 (71,32%) were the highest among other variables (>60%). Conclusions In COVID-19 patients, there was a correlation between elevated IL-6 and D-dimer levels with disease deterioration. There was no correlation between elevated IL-6 levels with ferritin, D-dimer, fibrinogen, and PT levels. Therefore, changes in IL-6 and D-dimer can predict worsening in moderate and severe COVID-19 patients.
Attitude towards dengue control efforts with the potential of digital technology during COVID-19: partial least squares-structural equation modeling
Authors: Purnama Sang Gede , Susanna Dewi , Achmadi Umar Fahmi , Eryando Tris , Wulandari Luh Putu Lila 
Published: November 2022

Background: Dengue fever is still a public health issue in Indonesia, and during the coronavirus disease 2019 (COVID-19) pandemic, integrated digital technology will be required for its control. This study aimed to identify the factors that influence attitudes toward dengue control concerning the potential application of digital technology. Methods: This was a cross-sectional survey, with 515 people willing to fill out an online questionnaire. The analysis was conducted using Partial Least Square-Structural Equation Modelling (PLS-SEM). There were 46 indicators used to assess attitudes toward dengue control, which are organized into six variables: the need for digital information systems, perceptions of being threatened with dengue, the benefits of dengue control programs, program constraints, environmental factors and attitudes in dengue control. Results: The source of information needed for dengue control was mainly through social media. There was a positive relationship between perception of environmental factors to perception of dengue threat, perception of program constraints, perception of program benefits, and perception of digital technology needs. Perception of program benefits and threatened perception of dengue have a positive relationship with perception of digital technology needs. Conclusions: This model showed the variables perception of digital technology and perception of benefits had a positive association with attitude towards dengue control.