bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigate the diagnostic accuracy of rapid influenza diagnostic kit method in comparison to the real time reverse transcription polymerase chain reaction
Authors: Jha Bimalesh Kumar , lav Rajan , Manandhar Krishna Das 
Published: August 2022

Abstract
Background: This study is cross-sectional and consecutive study carried out to find out the sensitivity and specificity of QuickVue rapid influenza diagnostic kit test with reference to real time RT-PCR.This study was conducted at National Influenza Center, Reference Laboratory, National Public Health Laboratory, Teku, Kathmandu. This study was undertaken in individuals having Influenza like illness visiting National Public Health Laboratory. Material & Methods: A total of 1683 throat swab specimens, obtained from patients with Influenza like Illness (ILI) at National Influenza Surveillance Network (NISN) sentinel hospitals, were transported to National Influenza Center, maintaining reverse cold chain, within 48 hours. Viral RNA was extracted using QIAmp viral RNA kit. Polymerase Chain Reaction assay (PCR) was performed following CDC Real-time rRTPCR protocol for detection and characterization of the influenza viruses including pandemic influenza virus A (H1N1) pdm 09. Samples were tested with quick view rapid test.Results: A total 1683 samples were processed for influenza testing by RDTQuickVue Influenza A+Band PCR simultaneously. Among 1683 samples 491 were positive by rapid testing and PCR as well. Whereas 122 were positive only by PCR. And out of 1520 negative samples, 1508 were negative by rapid testing & PCR as well and 12 were negative only by the PCR. Conclusion: In our study it shows overallgood positivepredictive and negative predictive value help to provide about influenza in quickly as point of care testing that help in the clinical management of the patient in the outbreak with less trained manpower and cost-effective kits. However more data and test need to be done to evaluate the clinical performance of RDT for influenza diagnosis on extrapolated to other age group & clinical information for specific symptoms and severity is require.
PVT1/miR-136/Sox2/UPF1 axis regulates the malignant phenotypes of endometrial cancer stem cells
Authors: Li Qing , Kong Fanfei , Cong Rong , Ma Jian , Wang Cuicui , Ma Xiaoxin 
Published: August 2022

Abstract
Background: Tumor stem cells (TSCs) are thought to contribute to the progression and maintenance of cancer. Previous studies have suggested that plasmacytoma variant translocation 1 (PVT1) has a tumor-promoting effect on endometrial cancer; however, its mechanism of action in endometrial cancer stem cells (ECSCs) is unknown. The purpose of this study was to explore the mechanism by which PVT1 regulates the malignant behavior and stemness of ECSCs.Methods: The expression of PVT1, microRNA-136 (miR-136), Y chromosome (SRY)-related high-mobility-group box 2 (Sox2), and Up-frameshift protein 1 (UPF1) in endometrial cancer tissues and ECSCs were detected by quantitative real-time PCR (qRT-PCR) and western blot analysis. The binding sites were predicted and confirmed by bioinformatics analysis, dual-luciferase analysis, chromatin immunoprecipitation (ChIP) assays, and qRT-PCR. Through in vitro and in vivo experiments, the regulatory role of the PVT1/miR-136/Sox2/UPF1 axis in endometrial cancer was investigated.Results: PVT1 and Sox2 were highly expressed in endometrial cancer and ECSCs. They correlated with poor patient prognosis, promoting malignant behavior, and the stemness of endometrial cancer cells (ECCs) and ECSCs. In contrast, miR-136, which is underexpressed in endometrial cancer, had the opposite effect. PVT1 competed with Sox2 to bind miR-136 and regulate the expression of UPF1, thereby exerting a tumor-promoting effect on endometrial cancer.Conclusion: The PVT1/miR-136/Sox2/UPF1 axis plays an important role in the progression and maintenance of endometrial cancer. The results suggest a novel target for endometrial cancer therapies.
The neural basis of panic-like behavioral and somatic symptoms and their rescue in mice
Authors: Kim Jong-Hyun , Kang Sukjae , Kim Dongil , Roberts Benjamin , Han Sung 
Published: August 2022

Abstract
Panic disorder (PD) is characterized by uncontrollable fear accompanied by somatic symptoms that distinguish it from other anxiety disorders. Neural mechanisms underlying these unique symptoms are not completely understood. Here we report that the pituitary adenylate cyclase-activating polypeptide (PACAP)-expressing neurons in the lateral parabrachial nucleus projecting to the dorsal raphe (DR) are critical for panic-like behavioral and physiological alterations. These neurons are activated by panicogenic stimuli, but inhibited in conditioned fear and anxiogenic conditions. Activating these neurons elicits strong defensive behaviors and rapid cardiorespiratory increase without creating aversive memory, whereas inhibiting them attenuates panic-associated symptoms. Chemogenetic or pharmacological inhibition of downstream DR PACAP receptor-expressing neurons completely abolishes panic-like symptoms, thus demonstrating that the pontomesencephalic PACAPergic pathway mediates panicogenesis, and thereby identifying a promising therapeutic target for treating PD.
Beneficial performance of a quasi-zero-stiffness vibration isolator with displacement-velocity feedback control
Authors: Cheng Chun , Hu Yan , Ma Ran , Wang Weiping 
Published: August 2022

Abstract
A displacement-velocity feedback control method is proposed to enhance the isolation performance of a quasi-zero-stiffness vibration isolator (QZS-VI). Time delay is considered in the controlled QZS-VI system. First, the steady-state solutions are obtained using the averaging method and further validated by a numerical method. The jump phenomenon and frequency island phenomenon could occur, and the stability analysis is implemented. Then, the effects of time delay and feedback gain on the frequency response and stability of solutions are analyzed in detail. And then, the force transmissibility is defined to evaluate the isolation performance of the controlled QZS-VI system. The results show that the time delay mainly affects the stability of the controlled system and has a weak influence on the isolation performance. The proposed displacement-velocity feedback control method can suppress the vibration in the resonant region effectively without affecting the performance in the isolation region. Finally, the vibration control effect is illustrated by the concept of an equivalent damping ratio.
Outer nuclear layer recovery as a predictor of visual prognosis in type 1 choroidal neovascularisation of neovascular age-related macular degeneration
Authors: Lee Seungheon , Kim Kyung Tae , Kim Dong Yoon , Chae Ju Byung , Seo Eoi Jong 
Published: August 2022

Abstract
Objectives
To investigate the changes in outer nuclear layer (ONL) thickness during anti-vascular endothelial growth factor (VEGF) treatment in type 1 choroidal neovascularisation (CNV) and its impact on vision.
Methods
Type 1 CNV eyes (n = 94) were retrospectively compared to normal control eyes (n = 35). Along with best-corrected visual acuity (BCVA), the location of CNV, foveal ONL thickness, and subretinal fluid height were measured using optical coherence tomography (OCT) and analysed. The type 1 CNV eyes group was subdivided into two groups based on ONL thickness changes during the initial three aflibercept loading doses. Visual outcome and OCT biomarkers were compared.
Results
The CNV group had thinner foveal ONL and worse BCVA compared to the control group. ONL thickness recovered partially along with visual improvement following aflibercept injections. Foveal ONL improvement correlated with the final BCVA during the 1-year follow-up. The study group was subsequently divided into two subgroups, based on the difference in foveal ONL thickness at baseline and after three loading doses of aflibercept: the poor response group (ONL change < + 10 µm; group A) and the good response group (ONL change ≥ +10 µm; group B). Group B showed better visual outcomes than group A, which had more subfoveal CNV (76.0%) than group B (45.5%, p = 0.012).
Conclusions
Type 1 CNV eyes that recovered following anti-VEGF treatment demonstrated good final visual outcome during the 1-year follow-up. Monitoring the foveal ONL thickness during early anti-VEGF treatment could predict visual outcomes in type 1 CNV patients.
Design and Dynamic Performance Research of MR Hydropneumatic Spring Based on Multi-physics Coupling Model
Authors: Jiang Min , Rui Xiaoting , Yang Fufeng , Zhu Wei , Zhu Hongtao , Han Wenjiao 
Published: August 2022

Abstract
Aiming at the problem that the damping coefficient of the traditional hydropneumatic spring cannot be adjusted in real-time, the magnetorheological (MR) damping technology was introduced into the traditional hydro-pneumatic spring with single gas chamber. A new shear-valve mode MR hydro-pneumatic spring was proposed. And its dynamic performance was analyzed based on multi-physical coupling simulation and mechanical property test. Firstly, a structural scheme of MR hydropneumatic suspension was proposed to ensure the original height adjustment function based on the working principle of traditional hydro-pneumatic suspension with single gas chamber. Secondly, based on the design requirements, the parameter of MR hydropneumatic spring damping structure was designed by using MR damper design method. Thirdly, the multi-physical coupling dynamic performance of the MR hydro-pneumatic spring damping structure was analyzed based on the electromagnetic field analysis theory, flow field analysis theory and thermal field analysis theory. The analysis results showed that the designed MR hydro-pneumatic spring has reasonable magnetic circuit structure and excellent working performance. Then, the mechanical properties of MR hydro-pneumatic spring were tested. The results showed that the maximum damping force can reach 20kN, and the dynamic adjustable multiple can reach 6.4 times. It has good controllability and meets the design requirements. Finally, a nonlinear model of MR hydro-pneumatic spring was established based on the elastic force calculation model of the gas and the Bouc-Wen model. The simulation results of the established model agree well with the experimental results, which can accurately describe the dynamic properties of the hydro-pneumatic spring. The proposed design and modeling method of the MR hydro-pneumatic spring can provide a theoretical basis for the related vibration damping devices.
The AuTOMATIC trial: a study protocol for a multi-arm Bayesian adaptive randomised controlled trial of text messaging to improve childhood immunisation coverage
Authors: Currie Grace E , Totterdell James , Bowland Grahame , Leeb Alan , Peters Ian , Blyth Chris C , Waddington Claire , Marsh Julie A , Snelling Thomas L 
Published: August 2022

Abstract
Background
While most Australian children are vaccinated, delays in vaccination can put them at risk from preventable infections. Widespread mobile phone ownership in Australia could allow automated short message service (SMS) reminders to be used as a low-cost strategy to effectively ‘nudge’ parents towards vaccinating their children on time.
Methods
AuTOMATIC is an adaptive randomised trial which aims to both evaluate and optimise the use of SMS reminders for improving the timely vaccination of children at primary care clinics across Australia. The trial will utilise high levels of digital automation to effect, including eligibility assessment, randomisation, delivery of intervention, data extraction and analysis, thereby allowing healthcare-embedded trial delivery. Up to 10,000 parents attending participating primary care clinics will be randomised to one of 12 different active SMS vaccine reminder content and timing arms, or usual practice only (no SMS reminder). The primary outcome is vaccine receipt within 28 days of the scheduled date for the index vaccine (the first scheduled vaccine after randomisation). Secondary analyses will assess receipt and timeliness for all vaccine occasions in all children. Regular scheduled analyses will be performed using Bayesian inference and pre-specified trial decision rules, enabling response adaptive randomisation, suspension of any poorly performing arms, and early stopping if a single best message is identified.
Discussion
This study will aim to optimise SMS reminders for childhood vaccination in primary care clinics, directly comparing alternative message framing and message timing. We anticipate that the trial will be an exemplar in using Bayesian adaptive methodology to assess a readily implementable strategy in a wide population, capable of delivery due to the levels of digital automation. Methods and findings from this study will help to inform strategies for implementing reminders and embedding analytics in primary health care settings.
Trial registration
: ANZCTR: ACTRN12618000789268
The Impact of COVID-19 on the Mental Health and Substance Use Health (MHSUH) Workforce in Canada: A Mixed Methods Study
Authors: Tulk Christine , Bartram Mary , Leslie Kathleen , Atanackovic Jelena , Chamberland-Rowe Caroline , Bourgeault Ivy 
Published: August 2022

Abstract
Background
The increased need for mental health and substance use health (MHSUH) services during the COVID-19 pandemic underscores the need to better understand workforce capacity. This study aimed to examine the pandemic's impact on the capacity of MHSUH service providers and to understand reasons contributing to changes in availability or ability to provide services.
Methods
We conducted a mixed method study including a pan-Canadian survey of 2,177 providers of MHSUH services and semi-structured interviews with 13 key informants. Survey participants answered questions about how the pandemic had changed their capacity to provide services, reasons for changes in capacity, and how their practice had during the pandemic. Thematic analysis of key informant interviews was conducted to gain a deeper understanding of the impact of the pandemic on the MHSUH workforce.
Results
Analyses of the survey data indicated that the pandemic has had diverse effects on the capacity of MHSUH workers to provide services: 43% indicated decreased, 24% indicated no change, and 33% indicated increased capacity. Logistic regression analyses showed that privately-funded participants had 3.2 times greater odds of increased capacity (B = 1.20, p < .001), and participants receiving funding from a mix of public and private sources had 2.4 times greater odds of increased capacity (B = 0.88, p < .001) compared to publicly-funded participants. Top reasons for decreases included lockdown measures and clients lacking access or comfort with virtual care. Top reasons for increases included using virtual care and more people having problems relevant to the participant's skills. Five themes were constructed from thematic analysis of key informant interviews: the differential impact of public health measures, increased inequity accessing MHSUH services, improving recognition driven by need for services, the accumulating impact of stressful working conditions, and critical gaps in MHSUH workforce data.
Conclusions
The COVID-19 pandemic has had a substantial impact on the capacity of the MHSUH workforce to provide services. Findings indicate the importance of increasing and harmonizing funding for MHSUH services across the public and private sectors, developing standardized datasets describing the MHSUH workforce, and prioritizing equity across the spectrum of MHSUH services.
An agent-based approach for aircraft fleet scheduling path planning considering space constraint
Authors: Cui Bowen , Tao Xiaochuang , Yuan Yanbin 
Published: August 2022

Abstract
In this paper, an agent-based approach for carrier based aircraft fleet scheduling path planning considering space constraint is analyzed and the objective is to minimize the fleet scheduling time. Firstly, the fleet scheduling environment is introduced, then modeling the aircraft fleet scheduling process based on multi-agent, two main agents are considered in this paper, Space Agent and Aircraft Agent. Next, a global optimization method for fleet cooperative path planning based on improved A* algorithm is given, which considering waiting strategy and bypass strategy, and the scheduling time is chosen as the optimization goal. Finally, the fleet with 10 carrier based aircraft is analyzed for verifying the proposed optimization approach. The simulation results show that the optimized algorithm can achieve the simultaneous scheduling of multiple aircraft, and the efficiency and the availability of the scheduling system is improved.
Association of platelet count trajectories with clinical outcomes in patients with sepsis: a retrospective cohort study based on the MIMIC-IV database
Authors: Wang Dong , Zhu Zepeng , Lu Xun , Mao Weipu , Chen Shuqiu 
Published: August 2022

Abstract
Background
Compared to normal platelet count (PC), absolute and relative platelet reductions are associated with worse clinical outcomes in septic patients. However, as to whether different variation trends of platelet count are associated with prognosis has not been investigated. The purpose of the research was to examine the effect of platelet count trajectories on prognosis and risk factors of septic patients with different PC trajectories.
Method
Group-based trajectory models (GBTMs), which identify groups of individuals on the basis of similar developmental paths, were used to identify three subgroups of PC trajectories (increasing, low, and medium PC). Hospital mortality and 90-day mortality were the primary outcomes. Multivariable Cox, logistic, linear regression analysis were used to assess relationship between PC trajectories and clinical outcomes and multinomial logistic regression were used to identify risk factors of different trajectories
Results
The proportion of patients with increasing, low, and medium PC was identified in 367 (6.2%), 3173 (53.4%), and 2406 (40.5%) patients. Compared with low PC, Medium PC was associated with lower hospital mortality [odds ratio (OR) 0.84, 95% confidence interval (CI) 0.72–0.99], the following relative risks (RRs) < 1: Male, Vasopressor use (1st 24h), RRT use (1st 24h), without hypertension, without diabetes, and CCU/CVICU
Conclusion
The Low PC trajectory was related to a higher risk of hospital mortality. Male, Vasopressor use (1st 24h), RRT use (1st 24h), without hypertension, without diabetes, and CCU/CVICU patients are risk factors of septic patients with low PC trajectories.
Inhibition of nucleophosmin/B23 sensitizes ovarian cancer cells to antiprogrammed cell death protein-1 ligand therapy
Authors: Lin Chiao-Yun , Tsai Chia-Lung , Tang Yun-Hsin , Chao Angel , Yang Lan-Yan , Wang Chin-Jung , Lai Chyong-Huey 
Published: August 2022

Abstract
Monoclonal antibodies against programmed cell death protein-1 (PD-1) and its ligand PD-L1 are immune checkpoint inhibitors (ICIs) that have been approved for the treatment of many human cancers. However, the response to these ICIs was limited in patients with ovarian cancer. Studies have indicated that the response to PD-1–PD-L1 blockade might be correlated with the PD-L1 expression level in cancer cells. Mechanistic pathways that control PD-L1 expression are yet to be elucidated and targeting these pathways can improve the efficacy of PD-1–PD-L1 blockade in patients with ovarian cancer. Our results revealed that NPM/B23 regulated PD-L1 expression in ovarian cancer cells. Mechanistically, NPM/B23 negatively regulates PD-L1 expression through NF-κB/p65. Moreover, in the immunocompetent mouse model, the NPM/B23 inhibitor/modulator sensitized the cancer cells to the anti-PD-1 antibody by regulating PD-L1 expression. Compared with treatment with the NPM/B23 inhibitor/modulator or the anti-PD-1 antibody alone, their combined use reduced tumorigenesis and increased CD8+ T-cell expansion, thus contributing to prolong the survival of MOSEC/Luc-bearing mice in vivo. In conclusion, targeting NPM/B23 is a novel therapeutic approach to sensitize cancer cells to immune therapy and reduce tumorigenesis.
Aligned nanofibrous collagen membranes from fish swim bladder as a tough and acid-resistant suture for pH-regulated stomach perforation and tendon rupture
Authors: Luan Zhaohui , Liu Shuang , Wang Wei , Xu Kaige , Ye Shaosong , Dan Ruijue , Zhang Hong , Shu Zhenzhen , Wang Tongchuan , Fan Chaoqiang , Xing Malcolm , Yang Shiming 
Published: August 2022

Abstract
Background
Wound closure in the complex body environment places higher requirements on suture's mechanical and biological performance. In the scenario of frequent mechanical gastric motility and extremely low pH, single functional sutures have limitations in dealing with stomach bleeding trauma where the normal healing will get deteriorated in acid. It necessitates to advance suture, which can regulate wounds, resist acid and intelligently sense stomach pH.
Methods
Based on fish swim bladder, a double-stranded drug-loaded suture was fabricated. Its cytotoxicity, histocompatibility, mechanical properties, acid resistance and multiple functions were verified. Also, suture’s performance suturing gastric wounds and Achilles tendon was verified in an in vivo model.
Results
By investigating the swim bladder's multi-scale structure, the aligned tough collagen fibrous membrane can resist high hydrostatic pressure. We report that the multi-functional sutures on the twisted and aligned collagen fibres have acid resistance and low tissue reaction. Working with an implantable "capsule robot," the smart suture can inhibit gastric acid secretion, curb the prolonged stomach bleeding and monitor real-time pH changes in rabbits and pigs. The suture can promote stomach healing and is strong enough to stitch the fractured Achilles tendon.
Conclusions
As a drug-loaded absorbable suture, the suture shows excellent performance and good application prospect in clinical work.
Improved mineralization of dental enamel by electrokinetic delivery of F- and Ca2+ ions
Authors: Tay NamBeng , Gan HiongYap , Sousa Frederico Barbosa , Shen Lu , Nóbrega Diego Figueiredo , Peng Chenhui , Kilpatrick-Liverman LaTonya , Wang Wei , Lavender Stacey , Pilch Shira , Han Jongyoon 
Published: August 2022

Abstract
This in vitro study evaluated the effects of the infiltration of F- and Ca2+ ions into human enamel by electrokinetic flow (EKF) on the mechanical property and F- content of enamel. Sound human enamel ground sections of unerupted third molars were infiltrated with de-ionized water by EKF and with F- ion by EKF respectively. All samples were submitted to two successive transverse acid-etch biopsies (etching times of 30 s and 20 mins) to quantify F- ion infiltrated deep into enamel. Remarkably, sound enamel showed a large increase in microhardness (MH) after infiltration of NaF (p < 0.00001) and CaCl2 (p = 0.013) by EKF. NaF-EKF increased AEC-LB remineralization compared to controls (p < 0.01). With enamel biopsy technique, at both etching times, more F- ions were found in EKF-treated group than the control group (p << 0.05) and more fluoride was extracted from deeper biopsies in the NaF-EKF group. In conclusion, our results show that EKF treatment is a superior in transporting Ca2+ and F- ions into sound enamel when compared to molecular diffusion, enhancing the mineralization of sound and carious human enamel.