bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Forecast Models for COVID-19 Hospital Admissions using Mobile Network Data: A Privacy-Preserving Approach
Authors: Taghia Jalil , Kulyk Valentin , Ickin Selim , Folkesson Mats , Nyström Cecilia , Ågren Kristofer , Brezicka Thomas , Vingare Tore , Karlsson Julia , Fritzell Ingrid , Harlid Ralph , Palaszewski Bo , Kjellberg Magnus , Gustafsson Jörgen 
Published: April 2022

Abstract
Reliable near-time forecast of COVID-19 hospital admissions can help enable effective resource management which is vital in reducing pressure from healthcare services. The use of mobile network data has come to attention in response to COVID-19 pandemic leveraged on their ability in capturing people social behaviour. Crucially, we show that there are latent features in irreversibly anonymised and aggregated mobile network data that carry useful information in relation to the spread of SARS-CoV-2 virus. We describe development of the forecast models using such features for near-time prediction of COVID-19 hospital admissions. In a case study, we verified the approach for two hospitals in Sweden, Sahlgrenska University Hospital and Södra Älvsborgs hospital, working closely with the experts engaged in the hospital resource planning. Importantly, the results of the forecast models were used in year 2021 by logisticians at the hospitals as one of the main inputs for their decisions regarding resource management.
A Novel Defined Risk Signature Based on Lactate-Related Genes can Predicts Prognosis of Bladder Cancer
Authors: Hu Ding , Jin Yanyang , Li Zizhi , Huang Weichao , Cao Qingfei , Tong Guangquan , Hong Peng , Shen Tong , Wu Weikang , Tong Ming , Ji Chundong 
Published: April 2022

Abstract
Background
Lactate is an important carbon source for cell metabolism as well as a signaling molecule in normal, chronic inflammatory and cancer tissues. However, the relationship between lactate and bladder cancer (BLCA) has not been studied yet.
Methods
Expression profiles of the patients with bladder cancer from The Cancer Genome Atlas were divided into different clusters using non-negative matrix factorization clustering to obtain differentially expressed genes (DEGs). Univariate Cox analysis, Lasso-Cox analysis, Kaplan-Meier survival analysis, and time-dependent receiver operating characteristics curves were used to developed and validated a prognostic signature for the survival rate of patients. "CIBERSORT" and "ESTIMATE" algorithms were used to quantify the immunocyte content and evaluate tumor purity for different clusters and subgroups. Online tool "Tumor Immune Dysfunction and Exclusion" was used to predict the immune escape ability of patients in different scoring subgroups after immunotherapy.
Results
The different clusters according to the expression pattern of lactate-associated genes (LAGs) had significant differences in the survival rate and the tumor microenvironment. Besides, a risk signature constructed by DEGs based on different clusters and verified by the GSE32894 dataset could not only significantly distinguish between the prognosis and clinical features of patients with different scores but was also found to be related to the tumor microenvironment and immunotherapy. Finally, a high accuracy nomogram model was established.
Conclusions
Overall, this research has revealed the relationship between lactate and patient prognosis, tumor microenvironment, and immunotherapy, which may provide novel prognostic biomarkers for patients with BLCA.
Prognostic Value of LDHD and Its Correlation with Immune Infiltrates in Thyroid Cancer
Authors: Zhong Jing , Tang Weiqiang , Chen Zuyao , Liao Yajie , Fang Yuan , Xia Min , Zu Xuyu , Wen Gebo 
Published: April 2022

Abstract
Background: LDHD is a subtype of the LDH family that can participate in D-lactate metabolism. However, the expression of LDHD in tumors and its role in tumor immunity are currently unknown. The objective of this research was to estimate the expression and prognosis of LDHD in pan-cancer and to explore the communication between LDHD expression level and tumor immune infiltration.Methods: LDHD expression level in pan-cancer was explored by TIMER and UALCAN databases and the prognostic value of LDHD was explored by Kaplan Meier plotter and PrognoScan databases. Then, the correlation between LDHD expression level and immune cell infiltration was evaluated by the TIMER database. In addition, the relationship between LDHD expression and clinical features of THCA was verified in the TCGA database. Results: The expression of LDHD mRNA or protein is downregulated in varied tumor types, and LDHD expression level is significantly related to the prognosis in tumors. Moreover, the downregulation of LDHD was found related to increased immune-cell infiltration and worse clinical features, especially in thyroid cancer.Conclusion: LDHD may be a potential pan-cancer prognostic biomarker. In thyroid cancer, loss of expression of LDHD may mediate tumor initiation and progression by affecting immune cell infiltration.
The Practices and Challenges in the Implementation of Participatory Teaching Strategies in English as a Foreign Language Large Classes in Ethiopia
Authors: Bulbula Dereje Asfaw , Areda Damtew Bulbula , Abajobir Fedila Abazinab , Bulti Tesfa Alemu 
Published: April 2022

Abstract
This study is aimed at investigating the Practices and Challenges in the Implementation of Participatory Teaching Strategies in Ethiopian EFL Large Classes. Large class to the context of this study is a class with many students in one class. To achieve this objective a descriptive research design with quantitative and qualitative data-gathering instruments and analysis was applied. This means questionnaire, observation and interview were a data-gathering instruments used to collect relevant data from grade 12 English language teachers and students. Two teachers were selected using Comprehensive sampling techniques and exposed to classroom observation and interview, whereas 20 students were also selected using simple random sampling techniques and filled the questionnaire. The questionnaires collected from sample students were analyzed quantitatively by using a descriptive statistical method. Teachers’ interviews were analyzed qualitatively. The findings of this study indicated that EFL teachers have a clear knowledge about the implementation of participatory teaching strategies in the classroom, yet they did not frequently use the diverse techniques. The most frequently used techniques of active learning strategies were pair and group work, question and answer, discussion method, and debating. Therefore, EFL teachers are limited to some techniques due to students’ number in the class, and this prominently hampers them not to practice various techniques of active learning strategies. Thus, EFL teachers, administrators, and curriculum experts should develop a clear awareness of the educational benefit of active teaching strategies and act accordingly.
Prokaryotic communities adapted to microhabitats on the Indian lotus (Nelumbo nucifera) growing in the high-altitude urban Dal Lake.
Authors: Rahi Praveen , Chaudhari Diptaraj , Kiran Shashi , Choudhary Ashish , Silveira Kris , Narwade Nitin , Dhotre Dhiraj , Khazir Jabeena , Mir Bilal Ahmad , Shouche Yogesh S 
Published: April 2022

Abstract
Indian lotus (Nelumbo nucifera) is one of the dominant aquatic plants cultivated in Dal Lake, situated at 1586 m above MSL in the northeast of Srinagar, Kashmir. Despite their economic and ecological role, the microbial communities associated with the lotus plant are still unexplored. In this study, we investigated prokaryotic communities harboured by different lotus microhabitats (roots, rhizome, leaves, flowers and fruits), lake water, and sediments using 16S rRNA gene amplicon sequencing. Overall, prokaryotic diversity decreased significantly on the lotus in comparison to the lake water and sediments. Among the microhabitats of lotus, roots and leaves harboured more diverse communities in comparison to rhizomes, fruits and flowers. A total of 98 genera were shared by lotus and the Dal Lake sediments and water. However, significant differences were found in their relative abundance, like Pseudomonas was the most dominant genus on most lotus microhabitats. On the other hand, Flavobacterium was highly abundant in the lake water, while a higher abundance of Acinetobacter was recorded in sediments. Additionally, significant differences were recorded in the proportion of several genera including Pseudomonas, Clostridium, Sphingomonas, Erwinia, Lactococcus, Rhizobium, Weissella, and Raoultella among the lotus microhabitats. Predicted functions of prokaryotic communities revealed a higher abundance of genes associated with nutrient uptake in the microhabitats of the lotus. This study offered first-hand information on the prokaryotic communities harboured by lotus plants and water and sediments of the Dal Lake and demonstrated the adaptation of diverse communities to microhabitats of lotus.
Optimized structural parameters and heat extraction capacity of mixing device for constant pressure CO2 mineralization using alkaline waste
Authors: Lu Wei , Yuan Yang , Hu Xiangming , Qi Guansheng , Sun Lulu , Zhang Maoyuan , Wang Mingjun , He Min 
Published: April 2022

Abstract
Alkaline waste such as calcium carbide slag is an ideal material for mineralizing CO2 and promoting atmospheric carbon reduction. In this study, the structural parameters of a mixing device and a thermal extraction method for the high-efficiency mineralization of CO2 using alkaline waste were optimized. First, the effects of various factors including the length–diameter ratio, blade inclination angle, spacing, and diameter in constant-pressure and continuous-feed systems on the degree of CO2 mineralization using carbide slag and on the extraction of the reaction heat during the mineralization process were investigated through experiments and numerical simulations. The influence of different configurations of the heat pipe and the internal fluid flow rate on extraction of the reaction heat was explored. The influence of different factors on the mineralization efficiency and heat extraction ability was analyzed based on the orthogonal simulation method. When the length–diameter ratio, blade inclination angle, spacing, and diameter of the mixing device were 3, 15, 6 cm, and 14 cm respectively, and a heat pipe was used, the heat extraction during calcium carbide slag (alkaline waste) mineralization of CO2 was at a maximum. In addition, the winding configuration of the heat pipe, which is beneficial for extracting more reaction heat, was optimal, and a model of the relationship between the heat pipe outlet water temperature and flow velocity at the outlet of the heat pipe was established. This study provides theoretical guidance for the field application of alkaline waste for high-efficiency mineralization of CO2, which can accelerate the realization of peak CO2 emissions and carbon neutrality.
Characterization of the complete mitochondrial genome of the new tea pest Mycterothrips gongshanensis (Insecta: Thysanoptera: Thripidae)
Authors: Li Shuai , Meng Zehong , Jin Daochao , Yang Wen , Zhang Jinfeng , Zhou Yufeng 
Published: April 2022

Abstract
Background Mycterothrips gongshanensis, as a new tea pest, has spread wildly in Guizhou and Yunnan Province, China, and the damage can be comparable to that of the major tea pest Dendrothrips minowai. Though its bionomics have been documented, but lack molecular biology researches. The present study, complete mitochondrial genome of M. gongshanensis was firstly obtained and analysed. Methods and Results We assembled the complete 15,154 bp circular mitochondrial genome of M. gongshanensis by the first-generation sequencing, which included 13 protein-coding genes (PCGs), 22 common tRNA genes, two repetitive tRNA genes (tRNA-Asn, and tRNA-Ile), two rRNA genes, and two control regions. The nucleotide composition is AT-biased (5.20%), with the respectively proportion as follow, 40.64% A, 12.67% C, 10.06% G and 36.62% T. The PCGs, with a whole length of 10,985 bp, the majority were initiated at typical start codons ATN (N, any nucleotide) and terminated with the typical stop codons TAA or TAG, except for atp8 using GTG and single T as start and stop codon, respectively. Additionally, the phylogenetic analysis indicated that M. gongshanensis was a sister group relationship with the genus Thrips, and the tribes Sericothripini and Dendrothripini were recovered as monophyletic group.Conclusions Gene rearrangement consists in the mitochondrial genome of M. gongshanensis, which is most closely related to the Thrips. The results will contribute to the classification and rapid identifucations of Thripidae.
Time trends and endoscopic management of foreign body ingestion in upper GI tract: An analysis of 1152 cases in China
Authors: zhang miaoxin , zhou qi 
Published: April 2022

Abstract
Background: Foreign body ingestions are encountered in the clinical practice and sometimes require endoscopic management. However, time trends and epidemiology of these cases have not been fully clarified. If seasons and festivals like Chinese New Year have an effect on the occurrence is poorly described.Methods: We enrolled consecutive 1152 foreign body ingestion cases in our endoscopic center from 2009 to 2020. Case records were reviewed for demographic data, foreign body type and location, outpatient or hospitalization, adverse events and dates. Annual time trends and seasonal variation were analyzed as well as influence of Chinese New Year on the incidence. Clinical features of these cases were demonstrated. Results: The overall success rate was 99.7% and adverse events rate was 2.4%. There was an uptrend in the annual frequency of food foreign body ingestion endoscopic extraction from 0.65 in 2009 to 8.86 in 2020 per 1000 patients of esophagogastroduodenoscopy (r=0.902, P<0.001). And the frequency of the endoscopic extraction had a significant increase in winter (P<0.001) and during Chinese New Year celebratory season (P=0.003). Inpatient(OR=5.656, P<0.001), jujube pits(OR=18.306, P=0.009) and age ≥60 years(OR=2.979, P=0.007) were risk factors of perforation and fistula.Conclusions: The annual frequency of endoscopic foreign body extraction in the upper gastrointestinal tract present an uptrend from 2009 to 2020. We should enhance the publicity about the danger of foreign body ingestion. Chinese New Year celebratory season and the winter may be the high-incidence period. Inpatient, jujube pits impaction and age ≥60 years may indicate higher risk of severe complications.
Pesticide level in chronic kidney disease patients: a single center study in Shanghai
Authors: Wu Xiaojing , Li Xin , Zhang Muyin , Xu Lili , Wang Weiming 
Published: April 2022

Abstract
This study was designed to investigating pesticide burden in Chinese Chronic Kidney Disease (CKD) patients having established etiology and find out the relationship between pesticide and CKD. We tested blood levels of pesticide in CKD patients due to glomerulonephritis (GN) or diabetic nephropathy (DN). Health controls were from physical examination center. Blood pesticides were analyzed by gas chromatography. The result showed the most common pesticide detectable in both CKD group and healthy controls were p, p’-DDE. The positive rate of blood p, p'-DDE was 55.5% in CKD group and 14.3% in healthy control group, with significant difference (c2=24.383, p＜0.01). The blood level of p, p'-DDE (median 2.4ng/ml, range 40.7ng/ml) in DN subgroup was significantly higher (p＜0.05) in comparison with GN subgroup (median 0.7ng/ml, range 17.2ng/ml). Blood p, p’-DDE showed significant negative correlation with eGFR (p=0.003, r=-0.263) and positive correlation with 24-hour urinary protein (p=0.013, r=0.22). Binary logistic regression showed p, p’-DDE（OR=3.211，95% CI 1.708-6.034）had a significant association with CKD, adjusting for age and Diabetes.
Hematopoietic stem cell-derived fibrocytes as targets and tools for cancer therapy: mechanisms and therapeutic implications
Authors: Zhang Liyi , Qiu Haibo , Zhang Xu , Qi Jiali , Zhang Jiangwen , Tong Yin , Li Lei , Fu Li , Qin Yan-Ru , Guan Xinyuan 
Published: April 2022

Abstract
Background: Cancer-associated fibroblast (CAF) is an ideal target for cancer treatment. Recent studies have focused on eliminating CAFs and their effects by targeting their markers or blocking individual CAF-secreted factors. However, these strategies have been limited by their specificity for targeting CAFs and effectiveness in blocking widespread influence of CAFs. Here, we aimed to explore the molecular mechanisms of CAF generation and optimize CAF-targeted therapeutic strategies.Methods: Using FGFR2 as a tracing marker, we identified a novel origin of CAFs in esophageal squamous cell carcinoma (ESCC). Furthermore, we successfully isolated CAF precursors from peripheral blood of ESCC patients and explored the mechanisms underlying their expansion, recruitment and differentiation via RNA-sequencing and bioinformatics analysis.Results: We found that FGFR2+ hematopoietic stem cell (HSC)-derived fibrocytes could be induced by ESCC cells, homing into tumor xenografts, and differentiate into functional CAFs. They could be mobilized by cancer-secreted FGF2 and recruited into tumor sites via the CXCL12/CXCR4 axis. Moreover, they differentiate into CAFs through the activation of YAP-TEAD complex which is trigger by directly contract with tumor cells. FGF2 and CXCR4 neutralizing antibodies could effectively block the mobilization and recruitment process of FGFR2+ CAFs. The YAP-TEAD complex-based mechanism can be used to locally activate the production of genetically encoded therapeutic payloads in tumor sites.Conclusion: We identified a novel CAF origin and systematically studied the process of mobilization, recruitment, and maturation of CAFs in ESCC under the guidance of tumor cells. These findings give rise to new approaches that target CAFs before their incorporation into tumor stroma and use CAF- precursors as cellular vehicles to target cancer cells.
Can green credit policy promote green total factor productivity? Evidence from China
Authors: Li Boyang , Zhang Jiawang , Shen Yue , Du Qiang 
Published: April 2022

Abstract
Green credit, a market-driven environmental policy instrument and an essential component of the green financial system, has piqued academic and policymakers' interest in whether it has successfully improved Chinese green total factor productivity (GTFP). Utilizing Chinese province panel data from 2006 to 2019, this study assesses GTFP using the Slack-Based Model with the Global-Malmquist-Luenberger technique and investigates the influence of green credit on GTFP as well as its mechanism. The findings suggest green credit has a favorable influence on China's GTFP. Green credit can boost GTFP through three mechanisms: upgrading industrial structure, stimulating green innovation, and optimizing energy consumption structures. Furthermore, green credit improves GTFP in eastern regions but has little impact elsewhere; the promotion impact is more effective in financial developed regions than in financial underdeveloped regions. As a result, the Chinese government should encourage regionally differentiated green credit policy implementation.
Observational study on the assessment of prognostic factors for mortality in patients with SARS-CoV-2 pneumonia
Authors: Spuntarelli Valerio , Taranto Aldo , Beumo Brice Ndongmo , Tartarone Emanuele , Bentivegna Enrico , Luciani Michelangelo , Morsa Alessandra , Bonfini Rita , Martelletti Paolo 
Published: April 2022

Abstract

Coronavirus disease 2019 (COVID-19) is a contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Infection can range from asymptomatic or paucisymptomatic to more severe forms with pneumonia and multiorgan dysfunction. Understanding the prognostic factors that influence the course of hospitalization and the onset of more severe forms of the disease has been one of the most important challenges of this pandemic. The main objective of this observational study is to establish some clinical and laboratory parameters useful for defining the prognosis of COVID-19 patients. The secondary end point is to understand how glycaemic changes affect the days of hospitalization. We observed how hypernatremia, hypocaliamia, newly diagnosed renal insufficiency and altered glycemic values were the main complications that arose during hospitalization, especially in patients staying in high-intensity units. Interestingly, we also noted how the altered glycemic values correlated with a longer length of hospital stay. We hope this study will help to better understand what the most important factors to be aware of for COVID-19 patients.
Tinea pedis epidemiology and importance from the lens of homelessness and low-resource settings.
Authors: Rasul Taha Faiz , Rafie Christine Isabelle , Anderson Jackson , Mathew Megan , Bergholz Daniel Richard , Henderson Armen 
Published: April 2022

Abstract
Patients experiencing homelessness (PEH) are a patient group with high levels of morbidity and mortality. PEH have high rates of dermatophyte infections, although the exact incidence and prevalence compared to the general population has not been determined. This study characterizes tinea pedis in homeless individuals across geographic locations and offers recommendations for treatment in low-resource settings. A total of 58 PubMed articles were analyzed with inclusion criteria containing ‘tinea pedis’ and ‘homeless’. Cohort studies, cross-sectional studies, and case-control studies published from 1979–2022 were evaluated. In North America, among sheltered homeless individuals the prevalence of tinea pedis ranged from 3.2–13.5% whereas it occurs at roughly a 10% frequency in the general population. European studies showed a tinea pedis frequency of 4.4–34.9% in the general population compared to 3.2% in a homeless patient cohort. There is incomplete data on tinea pedis infections in homeless populations in Africa, Asia, and South America. There are statistically significant differences in disease burden and healthcare utilization between sheltered and unsheltered homeless patients. This study raises questions about the status of dermatophytosis among homeless populations in high-risk areas such as the Southern United States. Pre-emptive treatment of tinea pedis is vital for PEH due to the potential for severe complications such as cellulitis and osteomyelitis. Longitudinal treatment in low-resource settings with simple interventions such as antifungal powder, socks, and hygiene supplies, can potentially prevent the progression of tinea pedis and improve disease burden.
A Novel Fungal Negative-strand RNA Virus Related to Mymonaviruses in Auricularia heimuer
Authors: Fu Yongping , Li Xufei , Liu Qingcheng , Li Shiyu , Sossah Frederick Leo , Han Xuerong , Zhu Guosheng , Li Yu , Li Changtian 
Published: April 2022

Abstract
We reported a novel (-)ssRNA mycovirus isolated from Auricularia heimuer CCMJ1222, using a combination of RNA-seq sequencing, reverse transcription-polymerase chain reaction, 5′ and 3′ rapid amplification of cDNA ends, and Sanger sequencing. Combining database searches, sequence alignment, and phylogenetic analysis, we designated the virus as Auricularia heimuer negative-stranded RNA virus 1 (AhNsRV1). The virus had a monopartite RNA genome related to mymonaviruses (Mononegavirales). The AhNsRV1 genome consists of 11,441 nucleotides and six linear open reading frames (ORFs). The largest ORF encodes a large protein; the second ORF encodes a hypothetical protein, while other ORF functions were unknown. AhNsRV1 was the first (-)ssRNA virus known to infect fungi and was the third virus to be discovered from A. heimuer.