bluuebunny/crossref_metadata_2025_split huggingface dataset:
COMPARISON OF ILLEGAL-UNREPORTED-UNREGULATED FISHING PRACTICES BASED ON PORT TYPE IN JAKARTA BAY
Authors: Ardisastra Ayi , Istiadi Yossa , Mansyur Umar 
Published: November 2022

An assessment on Comparison of Illegal-Unreported-Unregulated (IUU) Fishing practices based on type of port in Jakarta Bay conducted on 8-12 February 2021 in 2 (two) classes of fishing port namely PPS (Pelabuhan Perikanan Samudra) Nizam Zachman and PPN (Pelabuhan Perikanan Nusantara) Muara Angke. IUU Fishing violation practices is a fishing activity that is inconsistent and not in accordance with the conservation values and fisheries management, and violates international, regional, and national policies. Indonesia’s losses due to the practice of IUU fishing reach IDR 300 trillion annually or about 25% of Indonesia's total fishery potential. Aims of the assessment are to identify a violation rate of IUU Fishing, determine the value of IUU Fishing index and strategy for prevention and eradication of IUU Fishing practices in Jakarta Bay fishing port. Add the methods/analyse here … As a result of the studies showed that the highest of IUU Fishing violation rate occured at PPN Muara Angke which were about 184 of vessels compare with PPS Nizam Zachman which were only 63 vessels that contravene with rules. In addition, according to the index values of IUU Fishing at both fishing ports were moderate namely about 2,03 for PPS Nizam Zachman and 1,77 for PPN Muara Angke. Furthermore, based on the result of SWOT analysis that there are 3 (three) strategies to prevent and eradicate IUU Fishing practices consisted of: i) the strategy of optimization of integrated services which has a weight value of about 33,5%; ii) the strategy of establishment of law enforcement institution forum which has a weight value of about 31,3%; iii) the strategy of administration sanction for offender of unreported fishing which has a weight value of about 20,3%; and iv) the strategy of strengthening of vessels owner or patron (collectors) which has a weight value of about 15,0%
Hematophagy and tick-borne Rickettsial pathogen shape the microbial community structure and predicted functions within the tick vector, Amblyomma maculatum
Authors: Adegoke Abdulsalam , Kumar Deepak , Budachetri Khemraj , Karim Shahid 
Published: November 2022

BackgroundTicks are the primary vectors of emerging and resurging pathogens of public health significance worldwide. Analyzing tick bacterial composition, diversity, and functionality across developmental stages and tissues is crucial for designing new strategies to control ticks and prevent tick-borne diseases.Materials and methodsHere, we explored the microbial communities across the developmental timeline and in different tissues of the Gulf-Coast ticks (Amblyomma maculatum). Using a high-throughput sequencing approach, the influence of blood meal and Rickettsia parkeri, a spotted fever group rickettsiae infection in driving changes in microbiome composition, diversity, and functionality was determined.ResultsThis study shows that the core microbiome of Am. maculatum comprises ten core bacterial genera. The genus Rickettsia, Francisella, and Candidatus_Midichloria are the key players, with positive interactions within each developmental stage and adult tick organ tested. Blood meal and Rickettsia parkeri led to an increase in the bacterial abundance in the tissues. According to functional analysis, the increase in bacterial numbers is positively correlated to highly abundant energy metabolism orthologs with blood meal. Correlation analysis identified an increase in OTUs identified as Candidatus Midichloria and a subsequent decrease in Francisella OTUs in Rickettsia parkeri infected tick stages and tissues. Results demonstrate the abundance of Rickettsia and Francisella predominate in the core microbiome of Am. maculatum, whereas Candidatus_Midichloria and Cutibacterium prevalence increase with R. parkeri-infection. Network analysis and functional annotation suggest that R. parkeri interacts positively with Candidatus_Midichloria and negatively with Francisella.ConclusionWe conclude that tick-transmitted pathogens, such as R. parkeri establishes infection by interacting with the core microbiome of the tick vector.
Antibiotic ampicillin induces immune tolerance in renal transplantation by regulating the proportion of intestinal flora in mice
Authors: Wu Xiaoqiang , Cao Guanghui , Wang Zhiwei , Wu Xuan , Tian Xiangyong , Gu Yue , Shao Fengmin , Yan Tianzhong 
Published: November 2022

ObjectivesThere are significant differences in the composition of intestinal flora in renal transplant recipients before and after an operation, which has a great impact on the prognosis of renal transplantation. The purpose of this project is to study the effect of intestinal flora imbalance on renal transplantation.MethodsThe animal model of renal transplantation was established after intestinal flora imbalance (mice pretreated with compound antibiotics), or the animal model of renal transplantation was established after being pretreated with single antibiotics. HE, PAS, and Masson staining was used to detecting the histopathological changes of transplanted renal. The expression of inflammatory factors and infiltration of inflammatory cells of renal tissue were respectively been detected by ELISA kit and flow cytometry.ResultsAntibiotic pretreatment restored weight loss, and decreased serum creatinine level in mice after renal transplantation. The tissue staining, ELISA assay, and flow cytometry data showed that antibiotic pretreatment alleviated injury of the renal allograft, inhibited the inflammatory factors levels, and reduced inflammatory cell infiltration in mice after renal transplantation. Furthermore, single antibiotic, especially ampicillin pretreatment can also play the same role as compound antibiotics, such as restoring weight loss, decreasing serum creatinine level, alleviating renal allograft injury, inhibiting inflammatory factors levels, and reducing inflammatory cell infiltration in mice after renal transplantation.ConclusionsAntibiotic ampicillin may inhibit inflammatory cell infiltration after renal transplantation by regulating the proportion of intestinal flora in mice, to reduce renal injury and play a role in renal protection.
Glacial history and depositional environments in little Storfjorden and Hambergbukta of Arctic Svalbard since the younger dryas
Authors: Joe Young Jin , Jang Kwangchul , Forwick Matthias , Laberg Jan Sverre , Kong Gee Soo , Kang Moo-Hee , Yoon Seok-Hoon , Nam Seung-Il 
Published: November 2022

Geophysical and lithological data provide crucial information for the understanding of glacial history in Arctic Svalbard. In this study, we reconstructed the glacier-induced depositional environments of Little Storfjorden and its tributary, Hambergbukta, over the last 13 ka to better understand the glacial history of southeastern Svalbard. The combined uses of swath-bathymetry, high-resolution seismic stratigraphy, and multiple-proxy measurements of sediment cores allowed us to define five steps of glacier-induced depositional environments: 1) deposition of massive, semi-consolidated gravelly sandy mud (Facies 1) during re-advance or still-stand of the marine-based glaciers/ice streams in Little Storfjorden during Younger Dryas (13–12 ka); 2) deposition of massive mud to gravelly sandy mud (Facies 2A and B) during glacial retreat until the earliest Holocene (12–10.1 ka); 3) sediment winnowing by enhanced bottom currents during the early to middle Holocene (10.1–3.7 ka); 4) deposition of bioturbated sandy mud (Facies 3) with high productivity under seasonal sea ice conditions during the late Holocene (3.7–0.7 ka); and 5) deposition of (slightly) bioturbated sandy to gravelly mud (Facies 4) affected by glacier surges since Little Ice Age (LIA) (Facies 4). In addition to seismic stratigraphy, depositional patterns of IRD in Little Storfjorden indicate that the glacier surges in Hambergbukta occurred only after ∼0.7 ka. This suggests that the terminal moraine complex (TMC) represents the maximum extent of the LIA surges, which argues against the recent inference for the TMC formation during pre-LIA. This study shows the importance of multiple parameters to better understand the current behavior of tidewater glaciers in the Svalbard fjords in response to rapid climate change.
Gastroesophageal reflux related changes after sleeve gastrectomy and sleeve gastrectomy with fundoplication: A retrospective single center study
Authors: Aili Aikebaier , Maimaitiming Maimaitiaili , Maimaitiyusufu Pierdiwasi , Tusuntuoheti Yusujiang , Li Xin , Cui Jianyu , Abudureyimu Kelimu 
Published: November 2022

BackgroundThe worsening of gastroesophageal reflux disease (GERD) and “de novo” GERD after laparoscopic sleeve gastrectomy (LSG) is a major concern as it affects the patient’s quality of life; the incidence of GERD after LSG is up to 35%. Laparoscopic sleeve gastrectomy with fundoplication (LSGFD) is a new procedure which is considered to be better for patients with morbid obesity and GERD, but there is a lack of objective evidence to support this statement. This study aimed to assess the effectiveness, safety, and results of LSG and LSGFD on patients who were morbidly obese with or without GERD over an average of 34 months follow-up.MethodsFifty-six patients who were classified as obese underwent surgery from January 2018 to January 2020. Patients who were obese and did not have GERD underwent LSG and patients who were obese and did have GERD underwent LSFGD. The minimum follow-up time was 22 months and there were 11 cases lost during the follow-up period. We analyzed the short-term complications and medium-term results in terms of weight loss, incidence of de novo GERD/resolution of GERD, and remission of co-morbidities with follow-up.ResultsA total of 45 patients completed the follow-up and a questionnaire-based evaluation (GERD-Q), of whom 23 patients underwent LSG and 22 patients underwent LSGFD. We had 1 case of leak after LSGFD.No medium or long- term complications. The patient’s weight decreased from an average of 111.6 ± 11.8 Kg to 79.8 ± 12.2 Kg (P = 0.000) after LSG and from 104.3 ± 17.0 Kg to 73.7 ± 13.1 Kg (P = 0.000) after LSGFD. The GERD-Q scores increased from 6.70 ± 0.5 to 7.26 ± 1.7 (P = 0.016) after LSG and decreased from 8.86 ± 1.3 to 6.45 ± 0.8 (P = 0.0004) after LSGFD. The incidence of de novo GERD after LSG was 12 (52.2%) at the 12 month follow-up and 7 (30.4%) at the mean 34 (22–48) month follow-up. The remission of reflux symptoms, for patients who underwent LSGFD, was seen in 19 (86.4%) of 22 patients at 12 months and 20 (90.9%) of 22 patients at the mean 34 (22-48) month follow-up. The two groups did not have any significant difference in the effect of weight reduction and comorbidity resolution.ConclusionThe incidence of de novo GERD after LSG is high,LSG resulted in the same weight loss and comorbidity resolution as LSGFD, in patients who are morbidly obese and experience GERD, and LFDSG prevent the occurrence and development of GERD, combination of LSG with fundoplication (LSGFD) is a feasible and safe procedure with good postoperative results,which worthy of further clinical application.
Dysglycemia in young women attenuates the protective effect against fatty liver disease
Authors: Pérez-Montes de Oca Alejandra , Julián María Teresa , Pera Guillem , Caballería Llorenç , Morillas Rosa , Torán Pere , Expósito Carmen , Franch-Nadal Josep , Mauricio Didac , Alonso Nuria 
Published: November 2022

IntroductionSexual dimorphism has been reported in non-alcoholic fatty liver disease (NAFLD), similar to the sex differences evident with cardiovascular disease. Type 2 diabetes mellitus (T2D) significantly increases the risk and severity of NAFLD, but there is scarce information on whether T2D or altered glucose metabolism can modify the prevalence of NAFLD in men and women of reproductive age.PurposeTo investigate the relationship between age, sex and NAFLD in subjects with and without dysglycemia.Materials and methodsWe analyzed 2,790 patients. NAFLD was characterized using established diagnostic criteria: one or more positive results on the fatty liver index and hepatic ultrasound. Liver fibrosis (liver stiffness measurement [LSM] ≥8.0 kPa) was assessed by Fibroscan®. For analysis purposes, we included both T2D and prediabetes under the predefined condition of dysglycemia.ResultsThe global prevalence of NAFLD was higher in men than in women (50% and 34%; P&lt;0.001), and the prevalence increased with age in both sexes. Older women (≥ 50 years) had a higher prevalence than younger women (&lt;50 years), both in the overall cohort and in non-dysglycemic subjects. In dysglycemic subjects, the prevalence of NAFLD was slightly higher in men (68% vs 61%, p=0.021); in younger subjects, there were no differences in the prevalence of NAFLD between men and women (68% vs 64%, respectively; p=0.635). We found an interaction between dysglycemia and female sex (odds ratio [OR] 1.6 95% confidence interval [CI] 1.0-2.4, p=0.030), and between and age ≥50 years (OR 0.6, 95% CI 0.3-1.0, p=0.046). The global prevalence of LSM ≥8.0 kPa was higher in men compared with women (8% vs 4%; p&lt; 0.001). This prevalence increased with age, mainly in men. We did not find any association between liver fibrosis and age and gender.ConclusionsWhile the global prevalence of NAFLD is higher in men than in women across all ages, younger women with dysglycemia have a similar risk of developing NAFLD as men of a similar age. Therefore, the presence of dysglycemia may erase the protective effect of female sex against fatty liver disease.
Biochemical and molecular responses of the freshwater snail Pila sp. to environmental pollutants, abiotic, and biotic stressors
Authors: Panda Falguni , Pati Samar Gourav , Das Kajari , Samanta Luna , Sahoo Dipak Kumar , Paital Biswaranjan 
Published: November 2022

The semi-sessile, ectothermic, amphibious mollusc Pila globosa receives less attention for studying its environmental aspects. Its ecological role, ecotoxic and physiological responses to environmental factors including pollution and during dormant periods such as hibernation and estivation to understand key information about many cellular metabolic events need to be revealed. We reviewed the ecotoxic and physiological responses of P. globosa to various abiotic and biotic stressors such as heavy metals, organophosphorous, carbamate and butachlor in relation its adaptation to energy expenditure, fat metabolism in the dormant period, desiccation, etc. The responses analysed in terms of oxidative stress, respiratory and antioxidant enzymes, carbohydrate metabolism and activities of neurotransmitter enzymes such as acetylcholinesterase to better understand the connection between environmental factors and metabolic events in P. globosa. Articles published in the English language from 1952 to July of 2022 in PubMed, Google search engine and AGRICOLA that describes any of the above-mentioned search terms in P. globosa were included in the review article. Articles not containing any of the above terms were excluded. The 41 results were found in PubMed and 10 results from AGRICOLA and about 80 filtered articles from Google, totaling about 80 articles published until July 2022 were included in this review. The varied enzymatic activities observed in this organism under the exposure to various chemicals imply their possible use as biomarkers of eco-toxicological studies in freshwater and grassland ecosystems, for example, alleviated body carbohydrate and protein contents are up to 32% and 37% during aestivation and hibernation, respectively. Similarly, activities of enzymes such as adenosine monophosphate deaminase, adenosine deaminase, succinic dehydrogenase, cytochrome-c-oxidase, and glutamate dehydrogenase are downregulated by 75.5%, 62.6%, 54%, 59%, and 62%, respectively, during above inactive periods. Acetylcholinesterase enzyme is found to be the most susceptible enzyme to be downregulated by 225%, 130%, 64%, 198%, 96.08% in this snail under exposure to environmental pollutants such as phorate (30 mg/L), formothion (37.5 mg/L), trichlorfon (2.5 mg/L), aldicarb (120 mg/L) and butachlor (100 µ moles), respectively. In conclusion, P. globosa can serve as a reliable ectothermic model for comparative ecotoxicological studies in grassland and freshwater environmental monitoring.
Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps
Authors: Chen Wenhui , He Shaojuan , Xie Xinyu , Yang Xiaorong , Duan Chen , Ye Ping , Li Xuezhong , Lawrence Monica G. , Borish Larry , Feng Xin 
Published: November 2022

BackgroundChronic rhinosinusitis with nasal polyps (CRSwNP) is often characterized by recurrent nasal polyp (NP) growth following surgical removal, but the mechanisms are still not clear. This study aimed to investigate the expression of chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) receptor on NP and the role it plays in eosinophil inflammation and polyp recurrence.MethodsForty-one CRSwNPs patients and seventeen controls were enrolled in this study. mRNA was extracted from nasal tissues and evaluated for expression of CRTH2. Immunofluorescence staining was performed to confirm the distribution and expression of CRTH2 protein. CRTH2 expression on peripheral blood eosinophils was quantified by flow cytometry. The eosinophil count and clinical implications were also evaluated and their correlations with CRTH2 expression were analyzed.ResultsNasal polyps displayed increased expression of CRTH2 in mRNA level compared with control samples, with the highest expression observed in recurrent NP. Immunofluorescence confirmed over-expression of CRTH2 in recurrent NP and this was independent of the concurrent presence of asthma. CRTH2 expression was positively correlated with tissue eosinophil number (Spearman’s ρ=0.69,P&lt;0.001) and the postoperative sino-nasal outcome test-22 (SNOT-22) score (Spearman’s ρ=0.67,P&lt;0.001). Receiver operating characteristic (ROC) curves revealed CRTH2 was more predictive for NP recurrence compared to either eosinophil number and concomitant asthma, with an area under the ROC curve of 0.9107.ConclusionThe over-expression of CRTH2 in recurrent nasal polyps correlates with greater eosinophilic inflammation and poor prognosis which is independent of concomitant asthma.
HLA-associated outcomes in peanut oral immunotherapy trials identify mechanistic and clinical determinants of therapeutic success
Authors: Kanchan Kanika , Shankar Gautam , Huffaker Michelle F. , Bahnson Henry T. , Chinthrajah R Sharon , Sanda Srinath , Manohar Monali , Ling Hua , Paschall Justin E. , Toit George Du , Ruczinski Ingo , Togias Alkis , Lack Gideon , Nadeau Kari C. , Jones Stacie M. , Nepom Gerald T. , Mathias Rasika A. 
Published: November 2022

RationalePrevious studies identified an interaction between HLA and oral peanut exposure. HLA-DQA1*01:02 had a protective role with the induction of Ara h 2 epitope-specific IgG4 associated with peanut consumption during the LEAP clinical trial for prevention of peanut allergy, while it was a risk allele for peanut allergy in the peanut avoidance group. We have now evaluated this gene-environment interaction in two subsequent peanut oral immunotherapy (OIT) trials - IMPACT and POISED - to better understand the potential for the HLA-DQA1*01:02 allele as an indicator of higher likelihood of desensitization, sustained unresponsiveness, and peanut allergy remission.MethodsWe determined HLA-DQA1*01:02 carrier status using genome sequencing from POISED (N=118, age: 7-55yr) and IMPACT (N=126, age: 12-&lt;48mo). We tested for association with remission, sustained unresponsiveness (SU), and desensitization in the OIT groups, as well as peanut component specific IgG4 (psIgG4) using generalized linear models and adjusting for relevant covariates and ancestry.ResultsWhile not quite statistically significant, a higher proportion of HLA-DQA1*01:02 carriers receiving OIT in IMPACT were desensitized (93%) compared to non-carriers (78%); odds ratio (OR)=5.74 (p=0.06). In this sample we also observed that a higher proportion of carriers achieved remission (35%) compared to non-carriers (22%); OR=1.26 (p=0.80). In POISED, carriers more frequently attained continued desensitization (80% versus 61% among non-carriers; OR=1.28, p=0.86) and achieved SU (52% versus 31%; OR=2.32, p=0.19). psIgG4 associations with HLA-DQA1*01:02 in the OIT arm of IMPACT which included younger study subjects recapitulated patterns noted in LEAP, but no associations of note were observed in the older POISED study subjects.ConclusionsFindings across three clinical trials show a pattern of a gene environment interaction between HLA and oral peanut exposure. Age, and prior sensitization contribute additional determinants of outcomes, consistent with a mechanism of restricted antigen recognition fundamental to driving protective immune responses to OIT.
Five years follow up of patient receiving prolonged mechanical ventilation: Data for a single center in Taiwan
Authors: Huang Chienhsiu 
Published: November 2022

BackgroundThe National Association for Medical Direction of Respiratory Care recommended tracking 1-year survival rates (the most relevant outcome) in patients treated with prolonged mechanical ventilation. However, patients treated with prolonged mechanical ventilation had higher mortality rates within the first 2 years after weaning. More knowledge regarding long-term mortality would help patients, families, and clinicians choose appropriate interventions and make end-of-life decisions. In this investigation, we attempted to determine the rates of long-term mortality for all patients treated with prolonged mechanical ventilation over a period of 10 years.ObjectiveThe purpose of this investigation was to enhance the overall survival outcomes for patients receiving prolonged mechanical ventilation by identifying the factors affecting the 5-year mortality rates for these patients.DesignRetrospective observational study.Materials and methodsIn this retrospective study, we explored the influential factors related to the overall survival outcomes of all patients treated with prolonged mechanical ventilation. We enrolled every individual admitted to the weaning unit between January 1, 2012, and December 31, 2016. The length of survival for each patient was estimated from admission to the weaning unit until death or December 31, 2021, whichever came first. We analyzed the data to investigate the survival time, mortality rates, and survival curves in these patients.ResultsLong-term follow-up information was gathered for 296 patients who received prolonged mechanical ventilation. There was better mean survival times in patients treated with prolonged mechanical ventilation with the following characteristics (in order): no comorbidities, tracheostomies, and intracranial hemorrhage. Successful weaning, receipt of tracheostomy, an age less than 75 years, and no comorbidities were associated with better long-term overall survival outcomes.ConclusionProlonged mechanical ventilation patients had abysmal overall survival outcomes. Even though prolonged mechanical ventilation patients’ long-term survival outcomes are tragic, medical professionals should never give up on the dream of enhancing long-term outcomes.