bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of knee function after reconstruction with Posterolateral Corner injury and with or without Posteromedial Corner injury: a prospective cohort study
Authors: Zhu Yanlin , Fu Weili 
Published: June 2022

Abstract
Background
In the multiple ligament injury of the knee joint, apart from the anterior cruciate ligament and the posterior cruciate ligament, the Posterolateral Corner and the Posteromedial Corner are two structures that are easily overlooked. If not properly identified and repaired in one stage, the knee joint may be unstable. even failure of cruciate ligament reconstruction. The purpose of this article was to evaluate the effect of knee joint recovery after PLC with or without PMC injury.
Methods
From 2016 to 2020, we screened a total of 2564 patients, of which 292 patients met the inclusion and exclusion criteria. In the end, a total of 44 people completed the study. Follow-up was performed at 1, 3, 6, 9, and 12 months after surgery. We used pain visual analog scale (VAS)for pain, IKDC score, Lysholm score, Tegner score. Opti-knee (a portable motion analysis system) was used to evaluate the stability of knee joint at one year.
Results
The knee joint function of PLC group and PLC combined PMC group was better than that before operation at 3 or 6 months after operation. Except for IKDC at 9-month follow-up and Tegner score at 9-month and 12-month follow-up, there was no significant difference between the other groups.
Conclusions
PLC and PLC combined with PMC injury showed similar prognostic effects, although the PLC group was numerically superior to the other group. We recommend primary repair and reconstruction in patients with confirmed PLC and PMC injuries to achieve the best postoperative recovery.
Impact of Land use and Land cover change on stream flow of Beshilo watershed, Ethiopia
Authors: Eshete Asalf Shumete , Affessa Girum Metaferia 
Published: June 2022

Abstract
Land degradation is a series problem in Ethiopia highlands, particular in abbay basin reflected in the form of soil erosion and soil fertility decline from time to time. Beshilo catchment is one of the Abbay basin tributary, which covers 5,700 km2; this shows that the problem of the catchment is significant effect in Abbay basin development. In this study the impact of Land use/ Land cover change on annual outflow contribution of Beshilo Catchment is evaluated, distributed physically based hydrological model known as soil and water assessment tool (SWAT) model was used this study. Changes were analyzed and characterized impacts on surface runoff were evaluated. The model was calibrated and validated over the gauged upper reaches of catchments of Gummara River. The model was calibrated for the period from 1999–2008 and validated for the period from 2009–2014. The performance of the model was evaluated on the basis of performance rating criteria, coefficient of determination, Nash & Sutcliff efficiency. The overall performance of the models gives good result. For this study the land use land cover change scenario, were developed using its change. From the land cover change analysis results it was found that there has been a substantial decline of forest lands, shrub lands, grass lands and expansion of agricultural land. The SWAT modeling shows the result indicated that the mean annual stream flow were increase by 7.4% with 2004 LULC from 1996 LULC and increased by 6.8% with 2013 LULC from 1996 LULC.
Intermediaries in phosphotransfer: structural features discriminating hybrid histidine kinase Rec domains from response regulator homologs
Authors: Brüderlin Mitchell , Böhm Raphael , Hiller Sebastian , Schirmer Tilman , Dubey Badri 
Published: June 2022

Abstract
In two-component systems, the information gathered by the histidine kinase (HK) is relayed to the cognate response regulator (RR). Thereby, the phosphoryl group of the auto-phosphorylated HK is transferred to the receiver domain of the RR to allosterically activate its effector domain. In contrast, multi-step phosphorelays comprise at least one additional Rec domain (Recinter) that is part of the HK and acts as an intermediary for phosphoryl-shuttling. While RR Rec domains have been studied extensively, little is known about Recinter domains and their potentially discriminating features. As a bona-fide Recinter domain, here we have studied the C-terminal Rec domain of the hybrid HK CckA (CckARec) from Caulobacter crescentus by X-ray crystallography and NMR spectroscopy. CckARec exhibits the canonical Rec-fold, though with a degenerated α4 helix, in which all active site residues are pre-arranged for phosphoryl-binding. BeF3- binding does not alter secondary structure nor the oligomeric state, indicating the absence of allosteric changes, the hall mark of RRs. Based on structural modeling and sequence co-variation analysis, we present a detailed picture for the intra-molecular association of the CckA DHp/Rec domains and discuss the role of a FATGUY motif, a distinguishing feature of CckARec orthologs.
Characterization of thiamine pyrophospho kinase of vitamin B1 biosynthetic pathway as a drug target in Lesishmania donovani
Authors: Kumar Ravi Ranjan , Jain Ravi , Sharma Veena , Singh Shailja 
Published: June 2022

Abstract
Vitamin B1 is an essential cofactor required by carbohydrate-metabolizing enzymes such as Transketolases. Humans obtain these vitamins from their diet, although certain bacteria, yeast, plants, and lower eukaryotes synthesize them de novo. Because of the specificity of this system, the enzymes involved are amenable to therapeutic manipulation. In the final step of Thiamin Pyrophosphate (TPP) biosynthetic pathway, Thiamine Pyrophospho Kinase (TPK) converts Thiamin into its active form, TPP, opening an avenue to investigate the functional importance of this enzyme and the pathway's dispensability in Leishmania donovani, a protozoan parasite that causes visceral leishmaniasis. ldtpk gene encodes a 545 amino acid protein with a molecular mass of 58.4 kDa. LdTPK over-expression, antibody generation, cellular localization, enzyme kinetics, and inhibition assays with Oxythiamin and Pyrithiamin may provide a sound platform for the discovery of potential inhibitors for this enzyme. As a result, an in-depth analysis of the enzymes involved in TPP biosynthesis in L. donovani has the potential to generate novel therapeutic strategies for Leishmaniasis.
Analysis of strategy and efficacy clinical treatments of Kümmell disease
Authors: Xu Sheng , Ding Wei-guo , Sheng Hong-feng , Liu Jian , Song Hong-pu , Zhu Jia-fu , Xin Long , Xu Xin-wei 
Published: June 2022

Abstract
Objective: To investigate the most appropriate and effective clinical treatment strategies according to the characteristics of the different types of Kümmell disease. Methods: A retrospective analysis was performed of 48 patients with Kümmell disease treated in our hospital, for which complete follow-up data were available. The cases were evaluated according to Li classification, combined with clinical symptoms and imaging findings. The cases were classified into the following types. Type I: vertebral body height < 20% and no intervertebral disc degeneration from adjacent segments; Type II: vertebral body height loss > 20% and accompanied by degeneration or mild instability of intervertebral discs at adjacent segments; Type III: posterior vertebral cortical rupture and dural sac compression, and some accompanied by spinal cord nerve injury. Type III includes type IIIA (recoverable stable type), type IIIB (recoverable unstable type), type IIIC (spinal stenosis type) and type IIID (kyphosis type). There were 14 cases of type I (29.2%), 12 cases of type II (25%), 6 cases of type IIIA (12.5%), 7 cases of type IIIB (14.6%), 6 cases of type IIIC (12.5%), and 3 cases of type IIID (6.2%) in this study. Methods of surgery: The patients of Type I, II, and IIIA were treated with percutaneous vertebroplasty (PVP) or percutaneous kyphoplasty (PKP), type IIIB were treated with posterior fixation and fusion, type IIIC were treated with posterior decompression and fixation fusion, and type IIID were treated with posterior osteotomy, orthopedic fixation, and fusion. The VAS and ODI scores were followed up to examine whether the height of the injured vertebra had diminished or secondary kyphosis had occurred, and to detect any improvements in neural function. Results: All patients were followed up for 10–44 months (mean, 20.5 ± 4.5 months). The VAS and ODI scores of all patients were improved compared with those pre-surgery (P < 0.05). Eight cases (16.7%, 8/48) showed loss of vertebral height or secondary kyphosis. At the last follow-up, the AISA Grades of six patients with neural impairment were improved from preoperative Grade C to Grade D (one case), Grade D to Grade E (two cases) and Grade E (three cases). Conclusion: According to the characteristics of different types of Kümmell disease, appropriate clinical treatment strategies can achieve satisfactory curative effects and reduce the occurrence of complications.
Giardiasis and cryptosporidiosis in children and dogs, and the first report of assemblage E in northeastern Brazil
Authors: Harvey Tatiani Vitor , Carvalho Jamille Pinto dos Santos , de Aquino Monally Conceição Costa , Oliveira Bruno César Miranda , de Barros Luiz Daniel , Fehlberg Hllytchaikra Ferraz , Rocha Christiane Maria Barcellos Magalhães da , Albuquerque George Rego 
Published: June 2022

Abstract
Diagnosis is crucial for the control and prevention of diarrheal diseases such as giardiasis and cryptosporidiosis. The aim of this study was to determine the prevalence and genetically characterize isolates of Giardia duodenalis and Cryptosporidium spp. of 143 children and 193 dogs from 40 rural communities in northeastern Brazil. G. duodenalis cysts were concentrated by centrifugal-flotation/sedimentation. Molecular characterization of the isolates was carried out using the loci small subunit ribosomal RNA, beta-giardin, triosephosphate isomerase, and glutamate dehydrogenase for Giardia and with the locus 60-kDa glycoprotein for Cryptosporidium. By parasitological techniques, Giardia was detected in 72/192 (37.5%; 95% CI: 30.6%-44.7%) children and 24/139 (17.3%; 95% CI: 11.4%-24.6%) dogs. By molecular analysis, infection was detected in 60/141 children (42.5%; 95% CI: 34.3%-51.2%) and 26/92 dogs (28.3%; 95% CI: 19.4%-38.6%). The total prevalence of giardiasis, considering the result of parasitological and molecular techniques, was 54.4% (105/193; 95% CI: 47.1%-61.6%) in children and 32.9% (47/143; 95% CI: 25.2%-41.2%) in dogs. Zoonotic assemblages A and B of G. duodenalis were detected in children, and assemblage E of G. duodenalis was detected in one child and two dogs. Cryptosporidium was detected in 36/191 children (25.5%; 95% CI: 13.6%-25.1%) and 4/92 dogs (4.4% 95% CI: 1.2%-10.7%). Cryptosporidium parvum was detected in one children. The parallel use of parasitological and molecular techniques proves to be a more effective strategy for detecting giardiasis in endemic areas. This study is the first report of assemblage E in dogs from northeastern Brazil.
Tendency-and-Attention-Informed Deep Learning for ENSO Forecasts
Authors: Qiao Shen , Zhang Cuicui , Zhang Xuefeng , Zhang Kai , Shi Hao , Li Sheng , Wei Hao 
Published: June 2022

Abstract
Deep learning has been acknowledged as an increasingly important technology for ENSO forecasts. The most cutting-edge deep learning algorithm is developed based on Convolutional Neural Network (CNN), which can achieve a multi-year (about 17-month-lead) forecast and has conquered the ‘spring forecast barrier’ problem. However, this group of methods are still challenged by several critical issues. First, they usually utilize the global sea surface temperature (SST) fields as inputs without considering the specific contributions of variant oceanic regions in ENSO forecasts. Consequently, they cannot effectively investigate the role of the ‘teleconnection’ mechanism among different oceans (Indian, Pacific, and Atlantic Oceans) and different ocean parts (the tropic and non-tropic regions) especially in the forecast of extreme ENSO events. Second, existing methods mainly utilize the discrete monthly SST fields for Deep Learning for ENSO Forecasts ENSO forecasts without investigating the rate-of-changes between adjacent months, which also provides important information to the prediction of variation tendency. To solve these problems, this paper develops a Tendency-and-Attention-Informed Deep Residual Network (TA-DRN) for multi-year ENSO forecasts. The contributions of different oceanic regions can be learned by a spatial attention module while the variation tendency of adjacent previous and current months can be interpreted by the first-and-second order of differences of SST fields. Through informed by these two modules, the performance of TA-DRN can be improved significantly, especially in predicting extreme El Niño and La Niña events.
Heart Rate related to Accuracy of left ventricular strain parameters derived from the single-shot compressed sensing cine CMR images of patients with cardiovascular disease
Authors: Chen Yang , Xu Panpan , Wang Jun , Qian Wen , Liu Wangyan , Zhu Yinsu , Zhou Xiaoyue , Xu Yi , Zhu Xiaomei 
Published: June 2022

Abstract
Objective
To investigate the absolute and relative differences in the left ventricular (LV) global peak strain (GPS) values derived from single-shot compressed sensing (ss-CS) cine cardiac magnetic resonance (CMR) imaging and segmented cine CMR imaging (regarded as gold standard) of patients with cardiovascular disease, and investigate the potential patient-related factors that influence the misestimation of the GPS values from the ss-CS sequence.
Materials and Methods
Sixty-two cardiovascular disease patients with ss-CS cine and standard segmented cine CMR images were enrolled retrospectively. They were classified into different groups based on LV wall thickness and ejection fraction (EF). Absolute and relative differences in the LV GPS were calculated from both cine images. Stepwise multivariable linear regression analysis was performed to determine the patient-related factors that influenced the underestimation of the GPS values from ss-CS cine CMR images.
Results
LV GPS values from the ss-CS cine images were significantly underestimated. Absolute differences in the GPS values derived from both cine images varied significantly between the groups with different wall thickness and EFs (all P < 0.050). However, relative differences in the GPS values were not associated with the LV wall thickness or EF. The stepwise multivariable regression analysis showed that EF and heart rate were both associated with the absolute differences in the GPS values between the two cines (adjusted β-coefficient: 0.244 to 0.485, P < 0.05). Moreover, heart rate was independently associated with the relative differences in the global circumferential strain (GCS) and global longitudinal strain (GLS) values (GCS: β = 0.320, P = 0.011; GLS: β = 0.388, P = 0.002).
Conclusion
EF and heart rate were independently associated with the absolute differences in the LV GPS values. Heart rate was independently associated with the relative differences in the GPS values. Further improvements and standardization are necessary to increase the accuracy of the high-speed CMR imaging techniques in evaluating LV strain parameters.
Protective Effects of Quercetin/Mosses Extract (Homalothecium sericeum Hedw.) Combination on STZ-Induced Diabetic Rats
Authors: YAYINTAS Ozlem TONGUC , OZTURK Samil , Ceyda Latife , DEMİR Nesrin 
Published: June 2022

Abstract
Diabetes mellitus (DM) is the most common chronic endocrine disorder. Although new drugs and administration techniques for diabetes were developed, the nature of complications that occur chronically and reduce patients’ quality of life have not been entirely determined. In our study, 35 male rats were used. A single dose of 40 mg/kg streptozotocin was injected intraperitoneal into 28 rats, and a total of five groups were formed. Diabetes model was performed by applying 40mg/kg STZ. A diabetes model was not established in the subjects in the control and citrate buffer groups with STZ solvent. For treatment purposes, quercetin and moss extract were given by gavage method for three weeks. The liver and pancreas tissues were examined by histochemical and immunohistochemical methods. Immunohistochemically, Ki-67 (proliferation marker), antinuclear factor kappa (NF-κB), and interleukin 6 (IL-6) staining revealed significant differences in inflammation and regeneration in liver and pancreas tissues. In addition, the TUNEL staining method was used to determine apoptosis. Positivity was significant between the treatment and diabetes groups. In addition, it was determined that the agents used as treatment changed serum superoxide dismutase (SOD), malondialdehyde (MDA), C-peptide, insulin, and glucose levels biochemically with antioxidant and anti-apoptotic effects.
The effect of contact conditions on the performance of flexural seismic metasurfaces
Authors: Kaplunov Julius , Prikazchikova Ludmila , Wootton Peter , Nikonov Anatolij , Alzaidi Ahmed SM 
Published: June 2022

Abstract
Plane-strain motion of a flexural seismic metasurface in the form of a regular array of thin Kirchhoff plates attached to the surface of an elastic half-space is analysed. Two types of contact conditions, including simply supported plates and plates moving along horizontal rails are studies. Dispersion of time harmonic waves is investigated both asymptotically and numerically. A major effect of the contact conditions on metasurface behaviour is discovered. In particular, it is shown that frequency band gaps are not the feature of the array composed of simply supported plates. It is also demonstrated that the scaling laws, expressed through geometric and material problem parameters, drastically differ from each other for two considered setups.
Association between serum uric acid and relative handgrip strength compared using metabolic syndrome components
Authors: Yi Dongwon , Lee Min Jin , Khang Ah Reum , Kang Yang Ho 
Published: June 2022

Abstract
Background: This study investigated the association between serum uric acid (UA) and relative handgrip strength (HGS) using metabolic syndrome components.Methods: We analyzed the data of 5,579 Korean adults aged ≥20 years (2,486 men and 3,093 women) who participated in the Korea National Health and Nutrition Examination Survey VII (2018).Results: Among women, relative HGS was significantly lower in participants with hyperuricemia (1.61 ± 0.03) than in those without (1.92 ± 0.01) and was significantly decreased in the highest quartile (4Q:1.74 ± 0.02) of serum UA compared with that in the lowest quartile (1Q:1.96 ± 0.02). Among men, relative HGS was lower in participants with hyperuricemia (3.09 ± 0.03 vs. 3.15 ± 0.02) and decreased in the highest quartiles (4Q:3.08 ± 0.03) of serum UA compared with that in the lowest quartile (1Q:3.14 ± 0.03); however, these results were not statistically significant. In age- and multivariate-adjusted analyses in men, relative HGS was significantly lower in 4Q compared with that in 1Q in model 1 (adjusted for age), but there were no significant differences in model 2 (adjusted for age, body mass index, and waist circumference) and model 3 (adjusted for age, body mass index, waist circumference, systolic and diastolic blood pressure, fasting blood glucose, triglycerides and high-density lipoprotein cholesterol). Meanwhile in women, relative HGS was significantly decreased in 4Q compared with that in 1Q in all models.Conclusions: An inverse correlation was observed between serum UA levels and relative HGS. These results were particularly significant in women, and their significance was maintained even after adjusting for age and metabolic syndrome components.
Cardiac rehabilitation in coronary artery bypass grafting patients: Effect of 8-week of moderate-intensity continuous training versus high-intensity interval training
Authors: Shafie Neda , Kordi Negin , Gadruni Keivan , SalehFard Ziba , NazarAli Parvaneh 
Published: June 2022

Abstract
Purpose
The aim of the present study was to investigate the effects of traditional moderate-intensity rehabilitation training and interval training on some angiogenesis factors in coronary artery bypass graft (CABG) patients.
Methods
Thirty CABG patients (mean age ± SD, 55 ± 3 years) were randomly assigned to one of three groups: high-intensity interval training (HIIT) or moderate-intensity continuous training (MICT) or the control group. After the initial assessments, eligible patients in the experimental groups (HIIT and MICT) performed exercise training for 8 weeks, while the control group did not. Angiogenesis and angiostatic indices, including pro-adrenomedullin (pro-ADM), basic fibroblast growth factor (bFGF), and endostatin, were then measured.
Results
The results showed no significant difference between pro-ADM in the HIIT and MICT groups (P = 0.99), but a significant difference was found between MICT and the control group and between HIIT and the control group (P = 0.001). There is also no significant difference between the bFGF levels in the HIIT and MICT training groups (P = 1.00), but the changes in this factor between the training groups and the control group were significant (P = 0.001). There was a significant difference between the levels of endostatin in all three groups.
Conclusion
Two methods of cardiac rehabilitation (HIIT and MICT) may be useful for the recovery of patients with coronary artery bypass grafting. This improvement manifested itself in changes in angiogenesis and angiostatic indices in this study. However, more extensive studies are needed to investigate the effects of these two types of rehabilitation programs on other indicators of angiogenesis and angiostatic.
False positive analysis of machine-learning based sepsis prediction
Authors: Basu Debraj , Maharjan Jenish , Allen Angier , Thapa Rahul , Attwood Misty M , Hurtado Myrna , Iqbal Zohora , Hoffman Jana 
Published: June 2022

Abstract
Background
Despite the emergence of several promising machine learning models for prediction of patients at risk of sepsis, investigation of factors that contribute to false positive rates has not been performed. Here, we conducted a false positive analysis to determine the sources of false alerts and examine a mitigation methodology for reduction of false positive rates in sepsis prediction.
Methods
Analysis of false positive predictions from our sepsis prediction model was conducted and patient populations were stratified by underlying conditions that contribute to false positives. Sensitivity and specificity results for each subpopulation were calculated while adjusting the initial adjustment threshold. An optimal threshold was applied to an entire test set to observe the effect on performance.
Results
Certain underlying conditions mimic sepsis and contribute to false positive rates. The optimal threshold adjustment for the whole population resulted in greater reductions of false positives than true positives.
Conclusions
An optimal threshold adjustment to a whole population based on specific patient populations stratified by underlying conditions reduces the number of false positive cases while increasing the specificity and positive predictive value and still maintaining high sensitivity.