bluuebunny/crossref_metadata_2025_split huggingface dataset:
Competitive metal‐binding stoichiometry between calcium and strontium by cell wall proteins of Neurospora crassa
Authors: Kota Ashok K. , Mikkineni Anupama , Mathi Pardhasaradhi , Patnala Kiranmayi , Velagapudi Kavitha , Panditi Shravana K. , Jeevigunta Naveena L. L. 
Published: March 2022

AbstractCell wall proteins from Neurospora crassa were isolated and evaluated to demonstrate their metal ability to bind Ca2+/Sr2+ by loading the solubilized protein fraction on to immobilized metal affinity chromatography (IMAC) column pre‐equilibrated with Ca2+/Sr2+. The sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis IMAC eluent, revealed ∼18 proteins with a similarity in the proteome pattern of Ca2+/Sr2+ fractions. Diethyl aminoethyl chromatography showed five proteins in common in binding to Ca2+ and Sr2+, were subjected to N‐terminal sequencing. The sequence analysis was studied for the determination of metal‐binding site prediction by CHED software indicating that all five were found to have a high affinity toward Ca2+. From these five, two were randomly selected and denoted as CWP‐A (possess five Ca binding sites of six metal‐binding sites) and CWP‐B (possess six binding sites of eight metal‐binding sites). They were selected for further characterization studies to determine their Ca2+ bound Sr2+ binding properties. Surprisingly, these proteins were able to bind Sr2+ ions (29 μmol) with equal affinity as to Ca2+ ions (42 μmol) by means of direct binding, and/or by displacing calcium as observed in metal‐dependent proteolytic protection, fluorescence‐based metal exchange assays, and molecular simulation studies. From the results, we demonstrate for the first time, that there is a stoichiometry between Ca2+ (an essential macro elemental metal ion) and Sr2+ ions (a nonessential element for which no reported metabolic activity is reported) for the metal‐binding sites on cell wall proteins. This stoichiometry could be due to similar atomic dimensions and metal‐protein structure stabilizing properties of Sr2+ compared to Ca2+.
Tourist taxation as a sustainability financing mechanism for mass tourism destinations
Authors: Cárdenas‐García Pablo Juan , Pulido‐Fernández Juan Ignacio , Durán‐Román José Luis , Carrillo‐Hidalgo Isabel 
Published: March 2022

AbstractTourism taxation is one of the tools that can effectively contribute to obtaining resources that favor the development of policies to improve sustainability and the tourist experience in the destination. In this context, the objectives of this research are, on the one hand, to identify tourist preferences when offered 15 different taxes and fees, and, on the other hand, to identify which socio‐demographic factors and trip characteristics condition the tourist's willingness to pay (WTP) of the five most accepted taxes and fees. This study was carried out in Andalusia, a region in southern Spain with a mass tourism specialization.
Knowledge management and knowledge security—Building an integrated framework in the light of COVID‐19
Authors: Zieba Malgorzata , Bongiovanni Ivano 
Published: March 2022

AbstractThis paper presents a framework of knowledge risk management in the face of the COVID‐19 crisis, derived from the literature on knowledge management, knowledge security, and COVID‐19. So far, both researchers and practitioners have focused on knowledge as an asset and their efforts have been aimed at the implementation of knowledge management in various organizational contexts. However, with increasing threats related to cyberattacks or hazards associated with knowledge loss (as magnified by the COVID‐19 crisis), there is a growing need to account for knowledge‐related risks. In this conceptual paper, we integrate the contributions from the knowledge management and knowledge security fields, together with research on COVID‐19 to help organizations protect the knowledge they create, store and share. Based on a structured literature review, our investigation provides researchers and managers with a framework for securely handling organizational knowledge in a critical situation. Our framework revolves around two foci: one the one hand, building appropriate knowledge risk measures and controls; on the other hand, holistically tackling knowledge risks as part of knowledge management activities.
Complications of primary pediatric endoscopic sinus surgery for chronic rhinosinusitis: A 25‐year single surgeon experience
Authors: Ramadan Hassan H. , Bulbul Mustafa G. , Asad Fatima , Wasef Kareem , Makary Chadi A. 
Published: March 2022

AbstractObjectiveTo report our experience on the complications of primary pediatric endoscopic sinus surgery (ESS).MethodsCase series of pediatric ESS performed from 1991 to 2016 on children who failed maximal medical therapy and/or adenoidectomy. Inclusion criteria were children (age <12 years old) who underwent primary ESS with or without adenoidectomy for chronic rhinosinusitis (CRS) after failed maximal medical therapy and/or adenoidectomy. All patients underwent maxillary antrostomy ± partial or total ethmoidectomy. Patients with complicated acute rhinosinusitis were excluded. Complications reviewed included: skull base injury and CSF leak, orbital injuries (blindness, orbital hemorrhage, emphysema, periorbital swelling and bruising, fat exposure), and bleeding requiring intervention.ResultsA total of 352 patients underwent ESS between 1991 and 2016. There were no blindness or orbital hematoma reported, and no major nasal bleeding requiring intervention. The total number of complications was 31 (8.8%): 1 (0.3%) CSF leak, 3 (0.85%) orbital emphysema, 5 (1.4%) periorbital ecchymosis, and 22 (6.3%) lamina papyracea violation with orbital fat exposure.ConclusionsComplications of primary pediatric ESS can be rare dependent on surgeon's experience, the most common being orbital injury.Level of evidence: 4.
Commonalities and distinctions between two neurodevelopmental disorder subtypes associated with SCN2A and SCN8A variants and literature review
Authors: Mangano Giuseppe Donato , Fontana Antonina , Antona Vincenzo , Salpietro Vincenzo , Mangano Giuseppa Renata , Giuffrè Mario , Nardello Rosaria 
Published: March 2022

AbstractThis study was aimed to analyze the commonalities and distinctions of voltage‐gated sodium channels, Nav1.2, Nav1.6, in neurodevelopmental disorders. An observational study was performed including two patients with neurodevelopmental disorders. The demographic, electroclinical, genetic, and neuropsychological characteristics were analyzed and compared with each other and then with the subjects carrying the same genetic variants reported in the literature. The clinical features of one of them argued for autism spectrum disorder and developmental delay, the other for intellectual disability, diagnoses confirmed by the neuropsychological assessment. The first patient was a carrier of SCN2A (p.R379H) variant while the second was carrier of SCN8A (p.E936K) variant, both involving the pore loop of the two channels. The results of this study suggest that the neurodevelopmental disorders without overt epilepsy of both patients can be the consequences of loss of function of Nav1.2/Nav1.6 channels. Notably, the SCN2A variant, with an earlier expression timing in brain development, resulted in a more severe phenotype as autism spectrum disorder and developmental delay, while the SCN8A variant, with a later expression timing, resulted in a less severe phenotype as intellectual disability.
Multidisciplinary treatment for functional urological disorders with psychosomatic comorbidity in a tertiary pelvic care center—A retrospective cohort study
Authors: van Knippenberg Vera , Leue Carsten , Vrijens Desiree , van Koeveringe Gommert 
Published: March 2022

AbstractBackgroundFunctional urological disorders are highly prevalent, frequently interrelated, and characterized by a chronic course and considerable treatment resistance. From our point of view, poor treatment outcomes are often attributable to underlying but undetected mental disorders.ObjectiveTo investigate the effect of integrated outpatient care by a urologist and a psychiatrist on the symptomatology of patients with functional urological disorders in a tertiary referral Pelvic Care Centre.SettingRetrospective observational cohort study in functional urological disorders in combination with psychosomatic co‐morbidity. When treatment by a urologist alone was not sufficient, the suitability for a multidisciplinary approach was considered i) if there was a susceptibility for psychiatric comorbidity, ii) if diagnostic procedures did not reveal a treatable somatic cause, or iii) if multiple failed somatic treatments did not relieve complaints. Patients underwent urological treatments before, without reduction of complaints, no treatable somatic cause could be found after diagnostic procedures; or patients suffered from psychiatric comorbidity.MethodOutcome was measured using patient global impression of improvement, hospitality anxiety and depression scale (HADS), global assessment of functioning (GAF), and a health consumption questionnaire.ResultsA significant reduction in HADS‐depression score was found (p = 0.001) after multidisciplinary treatment. The GAF score increased from 61 to 80, leading to no more than slight impairment in social, occupational, or school functioning. Patients reported their situation as better in comparison with before multidisciplinary treatment. An association was found between pelvic pain and anxiety (p = 0.032) and panic disorder (p = 0.040). Psychological trauma was found to be associated with depression (p = 0.044), with an odds ratio of 2.93 (1.01–8.50). Psychological trauma coincided in 62.3% of patients with urological pain syndromes and in 83.3% with pelvic pain.ConclusionOverall results indicate that functional urological patients, previously refractory to urological treatment, benefit from an integrated care approach by urologists and psychiatrists. Explanation about the bladder–brain axis and the alarm falsification model enlightens understanding of urological and psychological contributions to functional syndromes and creates an opportunity for integrated care.
Caregiver burden and the associated factors in the family caregivers of patients with schizophrenia
Authors: Rahmani Farnaz , Roshangar Fariborz , Gholizadeh Leila , Asghari Elnaz 
Published: March 2022

AbstractAimOur study aimed to investigate the caregiving burden and its associated factors in family caregivers of patients with schizophrenia.DesignCorrelational study.MethodUsing the convenience sampling method, 215 caregivers were recruited from outpatient clinics affiliated with a tertiary referral psychiatric hospital in Iran. The caregiving burden was measured by the Zarit Burden Interview (ZBI‐22), and associations between caregiving burden and potential factors were examined using multiple regression analysis. We used the STROBE checklist to report the results.ResultsFamily caregivers of patients with schizophrenia reported a high level of caregiving burden, with 38.2% of the caregivers perceiving severe burden relating to their role. In the regression analysis, age, gender, educational level, income, job loss due to caregiving, relationship with patient, disease duration and frequency of caregiving were statistically significant predictors of caregiving burden. The regression model explained 54.4% of the variance of caregiving burden.
Audiovisual synchrony detection for fluent speech in early childhood: An eye‐tracking study
Authors: Zhou Han‐yu , Yang Han‐xue , Wei Zhen , Wan Guo‐bin , Lui Simon S. Y. , Chan Raymond C. K. 
Published: March 2022

AbstractDuring childhood, the ability to detect audiovisual synchrony gradually sharpens for simple stimuli such as flashbeeps and single syllables. However, little is known about how children perceive synchrony for natural and continuous speech. This study investigated young children's gaze patterns while they were watching movies of two identical speakers telling stories side by side. Only one speaker's lip movements matched the voices and the other one either led or lagged behind the soundtrack by 600 ms. Children aged 3–6 years (n = 94, 52.13% males) showed an overall preference for the synchronous speaker, with no age‐related changes in synchrony‐detection sensitivity as indicated by similar gaze patterns across ages. However, viewing time to the synchronous speech was significantly longer in the auditory‐leading (AL) condition compared with that in the visual‐leading (VL) condition, suggesting asymmetric sensitivities for AL versus VL asynchrony have already been established in early childhood. When further examining gaze patterns on dynamic faces, we found that more attention focused on the mouth region was an adaptive strategy to read visual speech signals and thus associated with increased viewing time of the synchronous videos. Attention to detail, one dimension of autistic traits featured by local processing, has been found to be correlated with worse performances in speech synchrony processing. These findings extended previous research by showing the development of speech synchrony perception in young children, and may have implications for clinical populations (e.g., autism) with impaired multisensory integration.
Dynamic material flow analysis of silicon photovoltaic modules to support a circular economy transition
Authors: Khalifa Sherif A. , Mastrorocco Benjamin V. , Au Dylan D. , Ovaitt Silvana , Barnes Teresa M. , Carpenter Alberta C. , Baxter Jason B. 
Published: March 2022

AbstractSolar photovoltaics (PV) are the fastest growing renewable energy technologies for clean, cheap, and sustainable electricity generation. To prepare for rapid scale‐up, the PV industry needs to project material requirements to build out all aspects of the supply chain appropriately and plan to handle large volumes of module waste. Impacts of deploying different material circularity strategies to reduce waste and conserve primary resources need to be quantified to inform sustainable material management. Here, we introduce the photovoltaic dynamic material flow analysis (PV DMFA) model based on PV electricity generation. The model quantifies material flows and stocks in the cradle‐to‐cradle life cycles of utility‐scale c‐Si PV systems in the United States through 2100. We present case studies for solar flat glass and aluminum frame materials under various scenarios to project the impacts of PV performance, reliability, and processing parameters, material circularity strategies, and module design shifts. In the absence of circularity measures, ~100 million MT of flat glass and ~12 million MT of aluminum would be needed for PV installations by 2100 to meet projected growth in domestic utility PV demand to nearly 1000 TWh in 2100. With optimistic but feasible improvements in efficiency, reliability, and circularity, material intensity and waste could be reduced by nearly 50%. Efficient module collection, minimally intrusive recycling, and careful scrap handling and cleaning could improve material circularity in the PV value chain. This model serves as a sustainability data support tool that may aid in the circular economy transition for PV systems.
Human peripheral blood mononuclear cells: A review of recent proteomic applications
Authors: Alexovič Michal , Lindner Joshua Raoul , Bober Peter , Longuespée Rémi , Sabo Ján , Davalieva Katarina 
Published: March 2022

AbstractHuman peripheral blood mononuclear cells (PBMCs) represent a sentinel blood sample which reacts to different pathophysiological stimuli in the form of immunological responses/immunophenotypic changes. The study of molecular content of PBMCs can provide better understanding of immune processes giving the possibility of monitoring the health conditions of the host organism. Proteomic analysis of PBMCs can achieve mentioned goal as important immune‐related biomarkers are easily accessible for analysis. PBMCs have been gaining attention in different research areas including preclinical or clinical investigations. In this review, recent applications of proteomic analysis of PBMCs are described and discussed. Approaches are divided based on different proteomic workflows such as in‐gel, in‐solution and on‐filter modes. The effect of various diseases such as autoimmune, cancer, neurodegenerative, viral, metabolic, and various immune stimulations such as radiation, vaccine, corticosteroids over PBMCs proteome, are described with emphasis on promising protein biomarker candidates.
Development of plasticity in vitrimers synthesized from a semi‐crystalline polymer using injection molding
Authors: Farge Laurent , Spiegel Rémi , André Stéphane , Noûs Camille , Lainé Richard , Hoppe Sandrine 
Published: March 2022

AbstractThe main objective of this work is to analyze finely the influence of cross‐linking on the mechanical properties of polybutylene terephtalate‐based vitrimers. Tensile tests were carried out at four temperatures (80, 100, 130, and 160°C) on a series of vitrimer specimens made by injection molding for which the cross‐linker concentrations were changed by small increments of 0.25%, from 0% to 2%. The displacement and strain fields were measured on the specimen surfaces through 3D digital image correlation in order to analyze the strain localization/delocalization phenomena that occur during the successive stages of the deformation process. In particular, we measured the yield strain (onset of strain localization), and the strain at neck stabilization (beginning of the strain delocalization phase). When the degree of cross‐linking increases, we observed two complementary effects leading to the decrease of the strain range during which plastic instability develops. Firstly increases. Secondly, and this is the main cause of the plastic instability strain range reduction, decreases. This latter effect results from the limitation in extensibility of the macromolecular network in the solid state caused by cross‐linking. More specifically, cross‐linking leads to two distinct modifications of the macromolecular network that both contribute to the reduction of its extensibility. The first is the decrease of the chain length between the network nodes. The second is the pre‐orientation of the macromolecular network that occurs during injection molding in the case of vitrimers with high viscosities due to cross‐linking. Eventually, when , the suppression of the yield point on the tensile curve was observed for the most cross‐linked vitrimers. Furthermore, in the temperature range of investigation, was found to be independent of temperature.
Human factor analysis and classification system for the oil, gas, and process industry
Authors: Yang Jeongmo , Kwon YoungGuk 
Published: March 2022

AbstractHuman error can cause severe problems in industrial oil, gas, and chemical processes. Although human factors analysis and classification systems (HFACSs) for the oil and gas industry (HFACS‐OGI) and the small‐scale chemical industry (HFACS‐CSMEs) have been independently developed, there are some limitations in providing detailed information on the contributing factors. Therefore, a new HFACS must be developed that can be commonly applied to the oil, gas, and chemical fields. This paper develops an integrated HFACS that can be applied to the oil, gas, chemical, and power plants, called HFACS‐OGAPI, by analyzing 45 accidents that occurred in domestic and overseas oil, gas, chemical, and power plants, based on the HFACS‐OGI system. An integrated HFACS‐OGAPI that can be applied to the oil, gas, chemical, and power plants is developed. The framework effectively identifies the contributing factors by providing specific information on Tiers 1, 2, 3, and 4 for Levels 1, 2, 3, 4, and 5. The contributing factors are effectively identified by analyzing the BP Texas Refinery explosion (2005), the Kleen Energy natural gas explosion (2010), and the Aghorn Operating Waterflood Station accidents (2019) with the developed framework. A checklist suitable for the HFACS‐OGAPI is developed by considering the contents developed by the National Aeronautics and Space Administration and the Department of Defense to determine the contributing factors. The developed checklist is expected to effectively identify and improve on the causes of human error through periodic inspection.
Design and experimental validation of a master manipulator with position and posture decoupling for laparoscopic surgical robot
Authors: Lei Yanqiang , Li Yibin , Song Rui , Wang Dechen , Zhang Tao , Zhang Gang , Du Fuxin 
Published: March 2022

AbstractBackgroundThe master manipulator with position and posture decoupling and force feedback can improve the immersion of the operation, and the gravity balance can reduce the fatigue of surgeons.MethodsA seven degree of freedom master manipulator is developed. The parallelogram structure and the angle conversion method contribute to the realization of decoupling position and posture. The calculating method based on the virtual work principle is adopted to achieve the passive gravity balance. The relationship between the master manipulator's joint torque and output force is established to achieve force feedback.ResultsA prototype of the master manipulator was built and its performance was experimentally evaluated. The maximum value of the positioning mean absolute error is 1.2 mm. The maximum absolute error of the force‐feedback is 0.32 N.ConclusionWith the functions of positioning, gravity balance, and force feedback, our manipulator shows the potential for single port laparoscopic surgery.
Event‐triggered controller design from the control input perspective
Authors: Xing Lantao , Lin Hong , Wang Chenliang , Liu Zhitao , Liu Meiqin 
Published: March 2022

AbstractIn this article, we propose a novel event‐triggered control (ETC) method from the control input perspective. Different from existing results in which event conditions are designed based on the variation of system states, this method designs event conditions from the control input perspective, that is, based on the control input variations. Through this method, it is shown that for nonlinear systems, the commonly used input‐to‐state stable assumption can be removed. For linear systems, this method is shown equivalent to conventional results established based on state signals. Moreover, it provides flexibility in designing different event conditions for uncertain nonlinear systems. The effectiveness of the proposed method is demonstrated through two simulation examples.
H∞$$ {H}_{\infty } $$ tracking control for uncertain switched stochastic systems with mixed time‐varying delays
Authors: Deng Yalin , Zhang Huasheng , Xia Jianwei , Sun Wei 
Published: March 2022

AbstractThis article concerns the exponential tracking control problem of uncertain switched stochastic systems with mixed time‐varying delays including discrete delay and distributed delay. First, according to the multiple Lyapunov–Krasovskii functional described by the mixed time‐varying delays and the average dwell time method, a delay‐dependent sufficient conclusion is obtained, which makes the augmented system mean‐square exponential stable and satisfies the tracking performance. Then, the corresponding controllers are designed to ensure that the system has model reference tracking performance based on the above stability analysis. Finally, a numerical example and water quality preservation problems are employed to corroborate the usefulness of the method.