bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic Use of Role-Playing Game (RPG) in Mental Health: A Scoping Review
Authors: Arenas Daniel Luccas , Viduani Anna , Araujo Renata Brasil 
Published: March 2022

Background Role-playing game (RPG) is a term that covers a series of forms and styles of games that involve, in some way, the creation, representation and progression of characters who interact in a fictional world under a system of structured rules. Its applications and effects on human behavior and mental health are, however, still an underexplored area. Methods A scoping review was performed on the literature about RPGs as a therapeutic tool or prevention strategy in psychotherapies and mental health, highlighting studies’ populations, forms of RPG and interventions used. To that, a systematic search in the PubMed/MEDLINE, Embase, PsycINFO, BVS/LILACS databases and grey literature was performed. Results Of the 4,069 studies reviewed, 50 sources of evidence were included. The majority was published as of 2011 (78%) in journals (62%) and targeted therapeutic uses of RPGs (84%). Most interventions used computer (50%) or tabletop RPGs (44%), mostly with cognitive and/or behavioral (52%) therapeutical approaches and targeting adolescents (70%). Conclusion The findings suggest a potential use of RPGs as a complementary tool in psychotherapies. However, only 16% of the studies included were experimental. We identified considerable heterogeneity in RPGs definitions, outcomes and interventions used, preventing a systematic review. Thus, more empirical and well-designed studies on the application of RPGs in mental health are needed.
Dynamic responses of the rail vehicle-bridge coupling system considering the spanwise correlation functions in vehicle aerodynamic forces
Authors: Hu Peng , Zhang Fei , Han Yan , Zhou Xuhui , Xu Guoji 
Published: March 2022

For rail vehicles, the spanwise correlation of aerodynamic forces acting on a single car is usually overlooked. To investigate the effects of spanwise correlation functions on the dynamic responses of the rail vehicle-bridge coupling system, firstly, the forms of spanwise correlation functions in the aerodynamic forces of a running vehicle, which are associated with the longitudinal and vertical fluctuating wind speeds, were proposed. Secondly, the spectra of buffeting forces acting on the running vehicle considering those spanwise correlation functions were obtained. Thirdly, a rail vehicle-bridge coupling system was established by using the commercial software SIMPACK, and a single car running on a typical high-speed railway bridge was taken as an example. Again, the effects of artificial discrete wind fields on the dynamic responses of the vehicle and bridge were investigated. Finally, the effects of the above spanwise correlation functions on the dynamic responses of the rail vehicle-bridge coupling system were compared under the cases of different vehicle speeds and wind speeds. The results show that different discrete time intervals can exert significant effects on the vehicle responses, and the discrete time interval of 0.05 s can be used for analysis in the wind-rail vehicle-bridge coupling system. With the increase of vehicle speed, the values of vehicle and bridge responses considering the spanwise correlation functions tend to be close to those without considering the spanwise correlation functions. However, as the wind speed increases, the values of vehicle and bridge responses generally tend to show greater differences in the presence and absence of spanwise correlation functions. At different vehicle speeds or wind speeds, the spanwise correlation functions have significant effects on the RMSs of vehicle accelerations and bridge lateral acceleration, but they have little effects on the bridge vertical acceleration.
Predisposing factors for the deformation of parent artery of anterior circulation saccular aneurysm after stent-assisted embolization: A retrospective cohort study
Authors: Lu Yuzhao , Ding Cong , Tan Song , Zhou Xiaobing , Wang Yang 
Published: March 2022

Background It has been previously reported in several studies that deformation of parent artery (DPA) occurs after stent-assisted coil embolization (SACE) for intracranial aneurysms (IAs). Objective To investigate the predisposing factors of stent-induced DPA, as well as its effect on the follow-up of aneurysm embolization. Methods Clinical and imaging data were collected from 198 patients (201 aneurysms). Angles of the stent-covered parental artery were measured before treatment and during follow-up. Cases in which the angle had changed more than 5 degrees, were defined as DPA. The related factors of DPA were analyzed. The relation between DPA and follow-up results was also studied. Results Univariate analysis revealed that sex (p = 0.014), age (p = 0.017), aneurysm location (p < 0.001), stent type (p < 0.001), aneurysm size (p = 0.019), and pretreatment angle (p = 0.002) correlated with DPA. On the other hand, multivariate analysis revealed that aneurysms located in the anterior communicating artery (ACOA) (OR = 4.559, p = 0.013) and middle cerebral artery (MCA) (OR = 9.474, p < 0.001) were independent predisposing factors for DPA after stent implantation, whereas a braided stent (OR = 0.221, p = 0.030), flow diverter (FD) device (OR = 0.100, p = 0.028) were negative factors to develop DPA. The complete occlusion rate in the DPA group was higher (p = 0.035) than in the non-DPA group. Conclusions Aneurysms located in ACOA and MCA are more prone to DPA after SACE than aneurysms at other locations in the anterior circulation, braided stents and FD devices do not predispose to induce vascular deformation. DPA may be beneficial for the long-term cure of IAs after SACE.
The adverse impact of pre-operative anaemia on survival following major lower limb amputation
Authors: Rammell James , Perre Daniel , Boylan Luke , Prentis James , Nesbitt Craig , Elmallah Ahmed , Nandhra Sandip 
Published: March 2022

Introduction Approximately 5000 major lower-limb amputations (MLLA) for PAD occur per-annum in the UK with clinical outcomes being poor for this high-risk cohort of patients. Existing evidence suggests that anaemic surgical patients have an increased 30-day mortality, but this has not been explored in the context of MLLA. Recent prioritization processes suggested that MLLAs are a target area for research into outcome improvement. This cohort study evaluates the impact of anaemia on the outcome of MLLA to understand if optimization might improve outcomes. Methods All PAD patients undergoing MLLA during 2015–2018 at a tertiary vascular centre were reviewed. Patients were stratified into groups; non-anaemia (>12 g/dL), mild-anaemia (12-10 g/dL) and severe-anaemia (<10 g/dL) by pre-operative haemoglobin (Hb). Primary outcome was overall survival by Kaplan–Meier. Secondary outcomes included length of stay (LOS), post-operative blood-transfusion, surgical-site infection (SSI) and myocardial infarction (MI). Cox-proportional-hazard and receiver-operator characteristics (ROC) analyses were conducted. Results 345 patients were followed up over (mean) 23 months. 105 were non-anaemic, 111 mildly anaemic and 129 severely anaemic. Patients with severe-anaemia had a higher incidence of heart and renal failure ( p = 0.003) than those with non- or mild-anaemia. Overall survival worsened significantly with increasing anaemia ( p = 0.001). LOS was significantly longer in mild-anaemia which is 26 (16–43) days, ( p = 0.006) and severe-anaemia of 28 days (17–40), ( p < 0.001) compared to non-anaemia of 18 (10–30) days. Post-operative blood-transfusion (RBC) was required more frequently in 70.5% of severely anaemic patients ( p < 0.001), compared to mildly anaemic (24.3%) and non-anaemic (7.6%) patients, with those receiving RBCs having a significantly worse survival. There was no difference in MI, SSI or wound dehiscence. Anaemia was significantly associated with mortality; (HR 1.7 (1.04–2.78), p = 0.03). A minimum-Hb of 10.4 g/L (by ROC) was identified as a cutoff Hb for an increased risk of mortality. Conclusion Pre-operative anaemia is associated with worse outcome following MLLA, with increasing severity of anaemia associated with increasing mortality and RBC transfusion being potentially detrimental. More work is required to prospectively evaluate this relationship in this complex and multi-morbid cohort of patients.
Biomechanical Analysis of the Ross Procedure in an Ex Vivo Left Heart Simulator
Authors: Bryan Amelia Y. , Brandon Strong E. , Kidambi Sumanth , Gilligan-Steinberg Shane , Bennett-Kennett Ross , Lee James Y. , Imbrie-Moore Annabel , Moye Stephen C. , Hendrickx-Rodriguez Sebastian , Wang Hanjay , Dauskardt Reinhold H. , Joseph Woo Y. , Ma Michael R. 
Published: March 2022

Background Neo-aortic pulmonary autografts often experience root dilation and valve regurgitation over time. This study seeks to understand the biomechanical differences between aortic and neo-aortic pulmonary roots using a heart simulator. Methods Porcine aortic, neo-aortic pulmonary, and pulmonary roots (n  =  6) were mounted in a heart simulator (parameters: 100 mm Hg, 37 °C, 70 cycles per minute, 5.0 L/min cardiac output). Echocardiography was used to study root distensibility (percentage change in luminal diameter between systole and diastole) and valve function. Leaflet motion was tracked with high-speed videography. After 30 min in the simulator, leaflet thickness (via cryosectioning), and multiaxial modulus (via lenticular hydrostatic deformation testing) were obtained. Results There were no significant differences between aortic and neo-aortic pulmonary leaflet motion, including mean opening velocity (218 vs 248 mm/s, P  =  .27) or mean closing velocity (116 vs 157 mm/s, P  =  .12). Distensibility was similar between aortic (8.5%, 1.56 mm) and neo-aortic pulmonary (7.8%, 1.12 mm) roots ( P  =  .59). Compared to virgin controls, native pulmonic roots exposed to systemic pressure for 30 min had reduced leaflet thickness (630 vs 385 µm, P  =  .049) and a reduced Young's modulus (3,125 vs 1,089 kPa, P  =  .077). In contrast, the aortic roots exposed to pressure displayed no significant difference in aortic leaflet thickness (1,317 vs 1,256 µm, P  =  .27) or modulus (5,931 vs 3,631 kPa, P  =  .56). Conclusions Neo-aortic pulmonary roots demonstrated equivalence in valve function and distensibility but did experience changes in biomechanical properties and morphology. These changes may contribute to long-term complications associated with the Ross procedure.
A multicenter retrospective study of polatuzumab vedotin in patients with large B-cell lymphoma after CAR T-cell therapy
Authors: Gouni Sushanth , Rosenthal Allison C. , Crombie Jennifer L. , Ip Andrew , Kamdar Manali K. , Hess Brian , Feng Lei , Watson Grace , Ayers Amy , Neelapu Sattva S. , Khurana Arushi , Lin Yi , Iqbal Madiha , Merryman Reid W. , Strati Paolo 
Published: March 2022

Abstract
Polatuzumab vedotin (PV) is an antibody–drug conjugate targeting CD79b that is approved for patients with relapsed/refractory large B-cell lymphoma (LBCL). Patients who relapse after chimeric antigen receptor (CAR) T-cell therapy were not included in the registration study, and reports of PV use after CAR T cells are limited. This multicenter retrospective analysis included patients with LBCL who relapsed or progressed after CAR T-cell therapy and subsequently received PV with or without rituximab and bendamustine between July 2019 and May 2021. Response to treatment and progression were assessed based on the 2014 Lugano criteria. Fifty-seven patients were included in the study: 18 (32%) patients were primary refractory to CAR T-cell therapy, and 34 (60%) patients received PV-based therapy immediately after CAR T-cell therapy. PV was combined with rituximab in 54 (95%) patients and administered with bendamustine in 35 (61%) patients. A response was achieved in 25 (44%) patients, including complete remission in 8 (14%). No significant association between baseline characteristics and response was observed. After a median follow-up of 47 weeks (95% confidence interval [CI], 40-54), 46 (81%) patients had disease progression or died, and the median progression-free survival was 10 weeks (95% CI, 5-15). On a multivariate analysis, bone marrow involvement (hazard ratio, 5.2; 95% CI, 1.8-15; P = .003) and elevated lactate dehydrogenase levels (hazard ratio, 5.0; 95% CI, 1.4-16; P = .01) were associated with shorter progression-free survival. Studies aimed at better characterizing the intrinsic mechanism of resistance and identifying optimal consolidation strategies for these patients are warranted.
Alkali-activation of cellulose nanofibrils to facilitate surface chemical modification under aqueous conditions
Authors: Yokota Shingo , Nishimoto Airi , Kondo Tetsuo 
Published: March 2022

AbstractIn this study, we developed a surface-activation technique for cellulose nanofibrils (CNFs) using mild-alkali and aqueous conditions. CNFs were initially processed using the aqueous counter collision (ACC) method to produce Janus-type amphiphilic CNFs with both hydrophilic and hydrophobic faces on the surface of a single nanofibril (ACC-CNF). Selective functionalization of the hydroxy groups on the hydrophilic faces creates an opportunity to develop novel nano-building blocks that introduce heterogeneous and tailored surface characteristics into the design of nanomaterials. In this study, alkaline conditions were used to activate the hydroxy groups on the surface of ACC-CNFs as a pre-treatment for the partial crystalline transformation from cellulose I to cellulose II. We found that alkali treatment with sodium hydroxide (NaOH) solutions (concentration range 1–7 wt%) did not fully transform the structure of ACC-CNFs into cellulose II, nor change the morphology of nanofibrils, as seen from their wide-angle X-ray diffraction patterns and atomic force microscopy images. We also found that the hydroxy groups at the surface region of the ACC-CNFs were sufficiently reactive under the moderate alkali and aqueous conditions to undergo subsequent carboxymethylation. Therefore, alkali treatment of ACC-CNFs with a 1–7 wt% NaOH solution rendered the surface of the ACC-CNFs as sufficiently reactive for chemical modification without morphological changes. This simple method for surface activation of CNFs can be useful in the development of future sustainable and novel materials for a variety of applications.
The use of monoclonal antibody therapy in pediatric patients with COVID-19: a retrospective case series
Authors: Santos Jesse De Los , Bhisitkul Donna , Carman Matthew , Wilson Kayla , Hasara Shannon , Homa Karen , Reyes Pedro , Bugajski Andrew , Barbera Andrew 
Published: March 2022

Abstract
Background
Monoclonal antibody (MCA) therapies have been utilized under emergency use authorization (EUA) for high-risk pediatric patients with mild to moderate coronavirus disease 2019 (COVID-19) in the outpatient setting since late 2019. The purpose of this study was to describe the use of MCA therapy in pediatric patients in the pediatric emergency department (ED) at a large community hospital.

Methods
This was a retrospective case series of high-risk pediatric patients 12 to 17 years of age who received MCA therapy in the pediatric ED between December 8, 2020 and June 3, 2021. The primary outcome was to describe the patient characteristics, clinical presentation, and safety profile of the pediatric population that received MCA therapy. The secondary outcome was to describe the incidence of hospitalizations or ED visits up to 28 days following therapy.

Results
A total of 44 patients were included in the analysis. The median number of days of symptoms was 4 with 41% of patients having symptoms between 0 and 3 days at time of MCA administration. Only one patient experienced a mild adverse event that did not require epinephrine administration. Two patients returned to the ED for reevaluation during the study follow-up period. No patients required admission within 28 days post-therapy.

Conclusions
The administration of MCA therapy in high-risk pediatric patients in the pediatric ED was well-tolerated with subjective improvement noted in COVID-19 symptoms post-therapy. Further studies are necessary to determine the role MCA therapy may play in reducing morbidity from COVID-19 infection in high-risk pediatric patients.
abc4pwm: affinity based clustering for position weight matrices in applications of DNA sequence analysis
Authors: Ali Omer , Farooq Amna , Yang Mingyi , Jin Victor X. , Bjørås Magnar , Wang Junbai 
Published: March 2022

AbstractBackgroundTranscription factor (TF) binding motifs are identified by high throughput sequencing technologies as means to capture Protein-DNA interactions. These motifs are often represented by consensus sequences in form of position weight matrices (PWMs). With ever-increasing pool of TF binding motifs from multiple sources, redundancy issues are difficult to avoid, especially when every source maintains its own database for collection. One solution can be to cluster biologically relevant or similar PWMs, whether coming from experimental detection or in silico predictions. However, there is a lack of efficient tools to cluster PWMs. Assessing quality of PWM clusters is yet another challenge. Therefore, new methods and tools are required to efficiently cluster PWMs and assess quality of clusters.ResultsA new Python package Affinity Based Clustering for Position Weight Matrices (abc4pwm) was developed. It efficiently clustered PWMs from multiple sources with or without using DNA-Binding Domain (DBD) information, generated a representative motif for each cluster, evaluated the clustering quality automatically, and filtered out incorrectly clustered PWMs. Additionally, it was able to update human DBD family database automatically, classified known human TF PWMs to the respective DBD family, and performed TF motif searching and motif discovery by a new ensemble learning approach.ConclusionThis work demonstrates applications of abc4pwm in the DNA sequence analysis for various high throughput sequencing data using ~ 1770 human TF PWMs. It recovered known TF motifs at gene promoters based on gene expression profiles (RNA-seq) and identified true TF binding targets for motifs predicted from ChIP-seq experiments. Abc4pwm is a useful tool for TF motif searching, clustering, quality assessment and integration in multiple types of sequence data analysis including RNA-seq, ChIP-seq and ATAC-seq.
Research on agroforestry systems and biodiversity conservation: what can we conclude so far and what should we improve?
Authors: Boinot Sébastien , Barkaoui Karim , Mézière Delphine , Lauri Pierre-Eric , Sarthou Jean-Pierre , Alignier Audrey 
Published: March 2022

AbstractThrough a meta-analysis, Mupepele et al. (BMC Ecol Evol 21:1–193, 2021) assessed the effects of European agroforestry systems on biodiversity, estimated by species richness or species diversity. They showed that the effects of silvoarable and silvopastoral systems depend on the systems they are compared to and the taxa studied. Further, they found that only silvoarable systems increased species richness or diversity, compared to cropland. The authors conclude that agroforestry systems have weak effects on biodiversity and that landscape context or land-use history are probably more important than the practice of agroforestry in itself. However, we draw attention to important shortcomings in this meta-analysis, which downplay the potential of agroforestry for biodiversity conservation in agricultural landscapes. We hope that the meta-analysis by Mupepele et al. (BMC Ecol Evol 21:1–193, 2021), and our comments, will contribute to improving the quality of research on agroforestry systems and biodiversity conservation.
Transcriptomic and proteomic analyses uncover the drought adaption landscape of Phoebe zhennan
Authors: Xie Na , Li Bo , Yu Jing , Shi Ruxia , Zeng Qin , Jiang Yunli , Zhao Dan 
Published: March 2022

Abstract
Background
Phoebe zhennan S.Lee (nanmu) is listed as a threatened tree species in China, whose growth and development, especially during the seedling stage, can be severely limited by drought. Previous studies on nanmu responses to drought stress involved physiological and biochemical analyses, while the molecular mechanisms remained unclear. Therefore, it is of great significance to carry out molecular biology research on the drought resistance of nanmu and reveal the genetic background and molecular regulation mechanism of nanmu drought resistance.

Results
Drought stress enhanced the soluble sugar (SS), free proline(PRO), superoxide anion (O2·−), and hydrogen peroxide (H2O2) contents as well as the peroxidase (POD) and monodehydroascorbate reductase (MDHAR) activities of nanmu. However, glutathione S-transferase (GST) activity was sensitive to drought stress. Further transcriptomic and proteomic analyses revealed the abundant members of the differentially expressed genes(DEGs) and differentially expressed proteins(DEPs) that were related to phenylpropanoid and flavonoid biosynthesis, hormone biosynthesis and signal transduction, chlorophyll metabolism, photosynthesis, and oxidation-reduction reaction, which suggested their involvement in the drought response of nanmu. These enhanced the osmotic regulation, detoxification, and enzyme-induced and non-enzyme-induced antioxidant ability of nanmu. Moreover, 52% (447/867) of proteins that were up-regulated and 34% (307/892) down-regulated ones were attributed to the increase and decrease of transcription abundance. Transcript up (TU) and protein up (PU) groups had 447 overlaps, while transcript down (TD) and protein down (PD) groups had 307 overlaps, accounting for 54% of up and 35% of down-regulated proteins. The lack of overlap between DEGs and DEPs also suggested that post-transcriptional regulation has a critical role in nanmu response to drought.

Conclusions
Our research results provide significant insights into the regulatory mechanisms of drought stress in nanmu.