bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of FABP5 suppresses mTOR -mediated autophagy via an increase in FASN to promote colorectal cancer progression
Authors: Ye Mujie , Chen Tiaotiao , Hu Chunhua , Bai Jianan , Zhong Yuan , Yan Lijun , Kan Jingbao , Chen Jinghao , Lu Feiyu , Li Xiaolin , Tian Ye , Tang Qiyun 
Published: September 2022

Abstract
Background
Lipid metabolism plays an important role in the occurrence and development of cancer, in particular, digestive system tumors such as colon cancer. Here, we investigated the role of the fatty acid-binding protein 5 (FABP5) in colorectal cancer(CRC).
Methods
To this end, tissue microarray was initially used for analysis of FABP5 expression, followed by generation of stable cell lines with knockdown or overexpression of FABP5 for a series of functional assays including CCK-8, colony formation, EdU and transwell experiments. Co-IP, RNA-seq and omics-based lipid metabolism studies were further performed to explore the mechanisms of action of FABP5 in CRC. The function of FABP5 in vivo was analyzed with the aid of tumor xenograft and immunohistochemistry experiments.
Results
We observed marked downregulation of FABP5 in CRC. Data from functional assays revealed inhibitory effects of FABP5 on cell proliferation, colony formation, migration, invasion as well as tumor growth in vivo. In terms of mechanistic insights, FABP5 interacted with fatty acid synthase (FASN) and activated the ubiquitin proteasome pathway, leading to a decrease in FASN expression and lipid accumulation, in turn, suppressing mTOR signaling and facilitating cell autophagy. Orlistat, a FASN inhibitor, exerted anti-cancer effects both in vivo and in vitro. Moreover, the upstream RNA demethylase ALKBH5 positively regulated FABP5 expression via an m6A-independent mechanism.
Conclusion
Our collective findings offer valuable insights into the critical role of the FABP5/FASN axis in tumor progression and uncover a potential mechanism linking lipid metabolism to development of CRC, providing novel therapeutic targets for future interventions.
Comparison of dexmedetomidine and dexamethasone as adjuvants to a single shot interscalene nerve block in arthroscopic shoulder surgery: A Bayesian network meta-analysis of randomized controlled trials
Authors: Liu Zheng , Lv Lian-chao , Wu Guang-han , Wei Xiu-min , Shi Peng-cai 
Published: September 2022

Abstract
Study objective: The objectives of this network meta-analysis were to determine the relative efficacy of dexamethasone and dexmedetomidine as an adjunct to prolong analgesic duration for a single shot ISB.
Design: A network meta-analysis was performed for all direct and indirect comparisons based on the Bayesian framework to select the best modality, including five interventions and 24 RCTs.
Setting: Operating rooms and wards.
Patients: Twenty-four randomized controlled trials involving 2,164 patients (ASA I-III) were included in the final analysis.
Interventions: Studies were summarized into six groups, a low-dose intravenous dexamethasone (4mg), a high-dose intravenous dexamethasone (8mg), perineural dexamethasone, dexmedetomidine, combined intravenous dexamethasone and dexmedetomidine, control group (CG) undergoing arthroscopic shoulder surgery.
Measurements: The primary outcome was duration of analgesia; the secondary outcomes were pain scores and opioid consumption.
Main results: Twenty-four 24 studies enrolling a total of 2,164 patients reported duration of analgesia. Combined DEX-DEA (MD = 18.48, 95% CI 13.14, 23.69), DEX-PN (MD = 9.75, 95% CI 7.51, 12.01), High dose-DEX-IV (MD = 7.39 95% CI 4.39, 10.38), Low dose-DEX-IV (MD = 7.15, 95% CI 3.44, 10.80), DEA-PN (MD = 6.51, 95% CI 3.57, 9.42), provided significantly longer analgesic effects compared to the CG group.
Conclusions: For prolonging duration of analgesia and reducing postoperative opioid consumption and pain scores, combination of dexamethasoneand dexmedetomidine had the best analgesic effects, peripheral dexamethasone was superior to other adjuvants administered alone. All therapies showed significant effects in prolonging the analgesic duration of a single shot interscalene nerve block in shoulder arthroscopic surgery when compared to placebo.
Depression and anxiety symptoms in older adults: a joint association study of candidate genes
Authors: de Moraes Isabela Ferreira , Chile Thais , de Paula Vanessa de Jesus Rodrigues , Alexandrino-Silva Clóvis , Busatto Gerado , Brentani Helena , Vallada Homero 
Published: September 2022

Abstract
Introduction: As the share of elderly in the
population is increasing, so is the presence of depression and
anxiety in this group, including in Brazil. There are studies
suggesting common pathophysiological mechanisms for depressive and
anxiety disorders, as well as the existence of vulnerability genes
in the etiopathogenesis of both depression and anxiety. The
different candidate genes reported in the literature associated
with depression and/or anxiety phenotypes have rarely been
investigated together in a single
study.

Objective: To investigate candidate gene
polymorphisms, reported as associated with a higher risk of
developing depression and/or anxiety symptoms in the literature, in
an elderly population.

Methodology: Peripheral venous blood was
collected from a total of 874 elderly people aged 60 years or
older. Genotypic DNA analysis was performed by real-time PCR of 27
polymorphisms of 11 candidate genes for symptoms of depression
and/or anxiety. Depressive and/or Anxious Symptomatology Groups
(DASG) were also included in the analysis based on the median of
three applied scales: the CES-D for depression, the GAI for
anxiety, and the MMSE for cognition. For statistical analysis,
Pearson's chi-square test was performed with a significance level
of 5% (p= ≤ 0.05), both for individual analysis of polymorphisms
and for the joint analysis.

Results: Four polymorphisms showed
statistically significant results associated with DASG: rs8071667
(p=0.03) of the 5HTT gene, rs6265 (p=0.004) of the BDNF gene,
rs165599 (p=0.023) of the COMT gene, and rs1417938 (p= 0.006) of
the CRP gene. The rs165599 (COMT) and rs1417938 (CRP) variants
remained significant when analyzed together, with a p-value of
1.72E-10.

Conclusion: The COMT gene variant rs165599 and
CRP gene variant rs1417938 provided the most robust results in our
analysis. However, it is necessary to confirm the reproduction of
these preliminary results in independent samples.
Interleukin-17A released from intestinal γδT cells induces cytokine storm in mice with severe dengue
Authors: Kurosu Takeshi , Okuzaki Daisuke , Sakai Yusuke , Kadi Mohamad Al , Phanthanawiboon Supranee , Ami Yasusi , Shimojima Masayuki , Yoshikawa Tomoki , Fukushi Shuetsu , Nagata Noriyo , Suzuki Tadaki , Kamimura Daisuke , Murakami Masaaki , Ebihara Hideki , Saijo Masayuki 
Published: September 2022

Abstract
Blockade of TNF-α signaling protects mice from lethal infection with dengue virus (DENV); however, what causes cytokine storm or how TNF-α aggravates the disease remains unknown. Here, we performed transcriptome analysis of the liver and small intestine samples collected chronologically from DENV-3 P12/08-infected IFN-α/β and γ receptor knockout (IFN-α/β/γRKO) mice in the presence/absence of blockade of TNF-α Ab signaling. We found that TNF-α induced production of IL-17A by small intestinal γδT cells, regulated production of other cytokines such as IL-6 cooperatively with TNF-α, and played a crucial role in mortality. Also, neutrophil-derived matrix metalloprotease-8 was identified as an effector molecule causing vascular leakage. Blockade of TNF-α or IL-17A signaling strongly inhibited nuclear translocation of NF-κB p65 in stroma-like cells and epithelial cells, and prevented induction of IL-6. These findings enable us to better understand cytokine storm associated with severe dengue, and may facilitate development of new therapeutic strategies.
Numeric and Morphological Studies of the African Lion (Panthera leo) Pectoral limb
Authors: Onwuama Kenechukwu Tobechukwu , Kigir Esther Solomon , Jaji Alhaji Zubair , Salami Suleiman Olawoye 
Published: September 2022

Abstract
Background: The Importance of the appendicular skeleton in the conformation, shape and physiology of wild animals especially carnivores for adaptation and survival cannot be overemphasized as limited and obsolete information on the bones of the forelimb necessitated this study. Matured adult African lions (Male and female) that weighed 173kg and 112kg were obtained at different times after post mortem examinations of their carcasses. Bone preparation was achieved via cold water maceration after proper skin and muscle removal to a barest minimum.
Results: The acromion process of the scapula consisted of a ventral hamate and caudal suprahamatus processes. The musculospiral groove of the humerus was more or less absent while its supracondyloid foramen and crest were positioned above the medial condyle and lateral condyle respectively. The radio-ulna presented a twisted appearance with the radius slightly curved thereby creating an extensive interosseous space that spanned its entire length. The seven (7) carpal bones were uniquely arranged in two rows while the 5 metacarpals anchored 5 digits with 3 phalanges except the first with 2 phalanges each. The 3rd phalanx had a unique appearance with a crescent plate projecting from the ventral cavity.The average total number of bones constituting the pectoral limb was 75.
Conclusion: Numerical information and detailed anatomical features of the pectoral limb bones of the African lion (Panthera leo) have added some valuable literature to science. This further serves as a baseline data for future scientific exposition on this species.
Setting the Stage for Success: Evaluating Both Partner and Patient Satisfaction with Post-Mastectomy Breast Reconstruction
Authors: Cimaroli Sawyer , Bichanich Miles , Adamson Karri , LoGiudice John , Doren Erin 
Published: September 2022

Abstract
Purpose: Few studies have looked in-depth at the relationship between patient and partner satisfaction with post-mastectomy breast reconstruction. The studies that do exist suggest that perceived partner satisfaction is an important predictor of patient satisfaction in post-mastectomy breast reconstruction.
Methods: We created a novel survey designed to look at reconstruction outcomes from a partner’s perspective. Patients with a history of mastectomy-alone or mastectomy with reconstruction at our institution from January 2011 through December 2020 were contacted electronically to complete a demographic form and the BREAST-Q©, while partners completed our novel partner survey. Sixteen mastectomy-only and 76 mastectomy with reconstruction couples completed surveys.
Results: The mean Breast-Q© and partner survey scores were 87 and 87 (maximum possible=100) respectively for mastectomy with reconstruction. There was a correlation of 0.57 between patient and partner overall satisfaction. There was no difference in overall satisfaction between patients who underwent reconstruction and those who did not p=0.19. There was a relationship between satisfaction with pre-operative counseling and overall satisfaction in both patients and partners. The partner survey was found to have high internal consistency for measuring various areas of partner satisfaction.
Conclusions: There is a relationship between patient and partner satisfaction with post-mastectomy breast reconstruction. Our data may support the positive impact of social support on recovery after breast cancer treatment, as satisfaction was high in both groups. We therefore encourage partners to attend and participate in pre-operative consultations. Finally, our novel partner survey is a reliable tool to assess partner satisfaction with post-mastectomy breast reconstruction.
Spatiotemporal Characteristics of Agricultural Carbon Emissions: Empirical Evidence from 31 Chinese Provinces
Authors: Tan Haixia , Zheng Xixian 
Published: September 2022

Abstract
A crucial part of China’s overall carbon emission reduction plan is the reduction of agricultural carbon emissions. Thus, it is important to identify the factors influencing agricultural carbon emissions in each province and explore the key to effectively reducing these emissions. Based on data from 31 provinces in China from 2007 to 2020, we analyzed the spatial and temporal trends in the evolution of agricultural carbon emissions in each province and determined the spatial autocorrelation of provincial carbon emissions. We further used the geographically and temporally weighted regression model to analyze the spatiotemporal evolution of the driving factors of provincial agricultural carbon emissions. The results showed that agricultural carbon emissions were spatially autocorrelated among provinces. The final results indicated that different provinces could achieve agricultural carbon emission reduction by adopting different strategies most suitable to their situations, such as by using green fertilizers, and adopting sustainability-oriented agricultural technology.
Gastrodin ameliorated features of MPTP-induced Parkinson's disease via activating VMAT2 maintained dopamine homeostasis
Authors: Zhao Meihuan , Xia Peng , Wang Jie , Li Ping , Ma Tengyun , Liu Panwang , Chen Qi , Xu Shijun 
Published: September 2022

Abstract
The vesicular monoamine transporter (VMAT2) plays a crucial role in maintaining dopamine (DA) homeostasis through packaged DA into vesicles, which has been suggested to being an excellent marker for presynaptic dopaminergic nerve terminals in the nigrostriatal of Parkinson's disease. Gastrodin (GTD), the major bioactive compound of Gastrodia elata, has shown neuroprotective in animal models for many neurological disorders. However, it is unclear whether GTD confers neuroprotection via activating VMAT2 to maintain DA homeostasis in an animal model of Parkinson's disease (PD) using the DA neuron-specific toxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Here, we first identified that GTD could ameliorate cell damage and the dysfunction of DA homeostasis in PC12 cells induced by MPTP via up-regulating VMAT2 expression. Moreover, GTD could enhance VMAT2 protein expression, increase striatal vesicle volume, and ameliorate DA dysregulation in MPTP-induced PD mice. Furthermore, we found that the DA homeostasis and therapeutic effect of GTD could be reversed by the VMAT2 inhibitor in vitro and in vivo. Finally, we confirmed that GTD could increase VMAT2 expression by activating MEK/ERK pathway. In summary, our data showed that GTD attenuated MPTP neurotoxicity through activating of the MEK/ERK/VMAT2 signaling pathway maintained DA homeostasis, suggesting that the manipulation of VMAT2 by GTD may provide a potential therapeutic strategy for PD.
Health Related Quality of Life and Associated Factors Among Patients With Urinary Bladder Cancer at Mulago National Referral Hospital- a Cross Sectional Study
Authors: IBINGIRA TREASURE J , ABRAHAMS OMODING , DAN NAMUGUZI 
Published: September 2022

Abstract
Background: Health related quality of life in patients with urinary bladder cancer is important to measure outcomes beyond morbidity and biological functioning. In 2020, Bladder cancer (BC) contributed to 3% of all cancer worldwide. Globocan 2018 estimated the prevalence of BC in Uganda at 0.8% with a mortality of 75.9%. BC affects the overall quality of life among patients with several factors influencing this outcome.
Our aim was to determine the overall health related quality of life and associated factors among patients with BC in our setting in MNRH in Uganda.
Methods: A sample of 111 patients, with histological diagnosis of BC, attending urology clinic or admitted to the urology ward in MNRH were recruited consecutively over a 4-month period. Data was collected by administering the EORTC -QLQ C-30 questionnaire which is a standard interviewer-administered, internationally accepted tool that is validated in Uganda in addition to an associated factors questionnaire. This tool assessed five domains, with symptoms scale and overall QOL. The mean and standard deviation of the overall quality of life were obtained to determine the mean HRQOL. Using simple linear regression, the factors associated with the mean HRQOL were assessed.
Results A total of 111 participants were analyzed and their mean age was 56.6 (SD± 17.3). Most were males 73 (65.8%) and most had attained a primary level of education 55 (49.5%). Most had no comorbidities 65 (58.6%). The mean HRQOL among patients with BC in MNRH was found to be 36.2%(±13.5). The mean duration with symptoms was 13.5 months (SD± 15.3)
Conclusion. Patients with bladder cancer in MNRH face a unique burden with their diagnosis and have been found to have a very low HRQOL which is significantly associated with increasing age and reduced duration of symptoms. This calls for timely interventions with holistic management and lifelong follow up of these patients.
High Dose of Metformin Decreases Susceptibility to Occlusive Arterial Thrombosis Uniquely in Type-2 Diabetic Mice
Authors: Alvidrez Roberto Mota , Annarapu Gowtham , Sriniva Amudan , Liu Zeyu , Yazdani Hamza , Nolfi-Donegan Deidre , Simmons Richard , Shiva Sruti , Neal Matthew 
Published: September 2022

Abstract
Introduction
Metformin is the most prescribed medication in Type 2 Diabetes(T2D). Metformin has shown to decrease mean platelet volume, with promising antiplatelet effects. High doses of Metformin have also been associated with hypercoagulation. We hypothesize that Metformin will protect T2D mice from occlusive arterial thrombus formation by altering platelet activation and mitochondrial bioenergetics.
Methods
T2D was developed by low dose of Streptozotocin, non-T2D (healthy) mice are controls. Either vehicle or Metformin was administered twice daily via oral gavage for 7-days. Ferric chloride (FeCl3) arterial thrombosis and tail bleeding time were performed. Whole blood aggregometry, platelet activation/adhesion and mitochondrial bioenergetics were evaluated.
Results
Metformin decreased susceptibility of T2D mice to arterial thrombosis. Platelet bioenergetics show T2D mice have increased platelet mitochondrial respiration, but no differences were observed with Metformin treatment. Metformin modulated ADP-dependent expression of markers of platelet activation/adhesion. Metformin in non-T2D mice shortens bleeding time and increased susceptibility to thrombotic occlusion. Metformin increased platelet mitochondrial maximal respiration and spare respiratory capacity uniquely in non-T2D mice.
Conclusion
Metformin regulates platelet bioenergetics and ADP-mediated platelet function in T2D mice which attenuates susceptibility to arterial thrombosis. Future studies will evaluate clinically relevant doses of Metformin that regulates thrombotic function in diabetic platelets.
Delay Reduction in MTC Using SDN Based Offloading in Fog Computing
Authors: Arefian Zahra , Khayyambashi Mohammad Reza , Movahhedinia Naser 
Published: September 2022

Abstract
Fog computing (FC) brings a Cloud close to the users, and improves the quality of service and delay services. In this article, the converged FC and Software-Defined-Networking (SDN) have been proposed to implement complicated mechanisms of resource management. SDN has suited the practical standard for FC systems. The priority and differential flow space allocation has been applied to arrange this framework for the heterogeneous request Machine-Type-Communications. The delay-sensitive flows are assigned to a configuration of priority queues on each Fog. Due to limited resources in the Fog, a promising solution is offloading flows to other Fogs a decision-based SDN controller. The flow-based Fog nodes have been modeled according to the queueing theory, where polling priority algorithms have been applied to service the flows and to reduce the starvation problem in a multi-queueing model. It is observed that the proposed mechanism notably reduces the computation and transmission delay and the count of the drops.
Actuator Fault Detection, Identification, and Control of a Multirotor Using Residual Generation and Parameter Estimation Approaches
Authors: asadi Davood 
Published: September 2022

Abstract
Effective fault detection and identification (FDI) and fault-tolerant control for nonlinear, unstable, and underactuated systems like quad-rotor is a challenging and critical process. This paper introduces a novel two-stage structure of an FDI approach integrated with an adaptive sliding mode controller for fault-tolerant control of a quadrotor with partial actuator fault. The FDI algorithm applies the parity space concept to generate a residual signal based on the system’s states and the inputs. The residual signal is examined by the exponential forgetting factor recursive least square method to detect and identify the partial fault of the actuator. The cascade controller includes an adaptive SMC algorithm in the inner loop and a PID controller in the outer loop. Real-time testbed experiments and Monte-Carlo simulation are applied in different actuator fault scenarios to determine the FDI algorithm’s performance metrics and demonstrate the effectiveness of the proposed algorithm. The results demonstrate the effectiveness of the fault-tolerant control strategy in maintaining full controllability of the quadrotor in presence of partial actuator fault.
Predictors of Preterm Neonatal Mortality in Hawassa University Comprehensive Specialized Hospital Neonatal Intensive Care Unit, Southern Ethiopia: a Retrospective Cohort Study
Authors: Feleke Tihun , Kaweti Gudeta 
Published: September 2022

Abstract
BACKGROUND: The issue of preterm neonates has paramount significance for achieving aims to end all preventable deaths of newborns and children below 5 years by 2030. Globally more than 1 million preterm neonates died out of 15 million babies born preterm every year and the problem is more significant in developing countries like Ethiopia.
OBJECTIVE: The aim of this study was to assess survival status and predictors of mortality among preterm neonates admitted to the Neonatal Intensive Care Unit of Hawassa University Comprehensive Specialized Hospital, Hawassa, Ethiopia, 2021
METHODS: An Institutional based retrospective cohort study was conducted in Hawassa University Comprehensive Specialized Hospital from May 9–30, 2021 after getting ethical clearance from the Institutional Review Board of Hawassa University College of Medicine and Health Sciences. The study population was all preterm neonates admitted to neonatal intensive care units (NICU) at Hawassa University Comprehensive Specialized Hospital (HUCSH) from May 9, 2019, to April 22, 2021. After reviewing different literature and neonatal national guideline, the data abstraction tool was developed and utilized after making some modifications. After collection, data were entered into epi data software version 3.1 and exported to STATA for data cleaning and further analysis. The level of significance was set at p<0.05 with the corresponding confidence intervals at 95% confidence. The Cox proportional hazard ratio was used to assess the association between dependent and independent variables.
RESULTS: The magnitude of preterm neonatal mortality was 33.3%. The final model revealed that early initiation of breast milk (AHR: 0.43 (95% CI: 0.29, 0.62), Early neonatal sepsis at admission (AHR: 1.34(95% CI: 1.003, 1.79), 5th minute Apgar score of less than 7 (AHR: 1.73(95% CI: 1.17, 2.55), perinatal Asphyxia (AHR: 2.25(95% CI: 1.67, 3.02) and recent multiple pregnancies (AHR: 1.66(1.22, 2.26) were significantly associated predictors of preterm neonatal mortality.
CONCLUSION: Preterm neonatal mortality remains a significant health problem in Hawassa University Comprehensive Specialized Hospital. It is basic to revamp the current work procedures and practices through the improvement of antenatal care, early breastfeeding, and timely detection of perinatal asphyxia, early neonatal sepsis, and hypertension