bluuebunny/crossref_metadata_2025_split huggingface dataset:
Highly-automated, high-throughput replication of yeast-based logic circuit design assessments
Authors: Goldman Robert P , Moseley Robert , Roehner Nicholas , Cummins Breschine , Vrana Justin D , Clowers Katie J , Bryce Daniel , Beal Jacob , DeHaven Matthew , Nowak Joshua , Higa Trissha , Biggers Vanessa , Lee Peter , Hunt Jeremy P , Mosqueda Lorraine , Haase Steven B , Weston Mark , Zheng George , Deckard Anastasia , Gopaulakrishnan Shweta , Stubbs Joseph F , Gaffney Niall I , Vaughn Matthew W , Maheshri Narendra , Mikhalev Ekaterina , Bartley Bryan , Markeloff Richard , Mitchell Tom , Nguyen Tramy , Sumorok Daniel , Walczak Nicholas , Myers Chris , Zundel Zach , Hatch Benjamin , Scholz James , Colonna-Romano John 
Published: October 2022

Abstract
We describe an experimental campaign that replicated the performance assessment of logic gates engineered into cells of Saccharomyces cerevisiae by Gander et al. Our experimental campaign used a novel high-throughput experimentation framework developed under Defense Advanced Research Projects Agency’s Synergistic Discovery and Design program: a remote robotic lab at Strateos executed a parameterized experimental protocol. Using this protocol and robotic execution, we generated two orders of magnitude more flow cytometry data than the original experiments. We discuss our results, which largely, but not completely, agree with the original report and make some remarks about lessons learned.
Graphical Abstract
Risk of Adverse Pregnancy Outcomes Among Pregnant Individuals With Gestational Diabetes by Race and Ethnicity in the United States, 2014–2020
Authors: Venkatesh Kartik K. , Lynch Courtney D. , Powe Camille E. , Costantine Maged M. , Thung Stephen F. , Gabbe Steven G. , Grobman William A. , Landon Mark B. 
Published: October 2022

(Abstracted from JAMA 2022;327:1356–1367)
In 2016, gestational diabetes mellitus (GDM) affected 6% of pregnancies in the United States. In 2015, maternal and neonatal adverse outcomes occurred in approximately 33% of pregnancies affected by GDM and in approximately 25% of infants born from pregnancies affected by GDM.
Single Nucleotide Polymorphism Array Versus Karyotype for Prenatal Diagnosis in Fetuses With Abnormal Ultrasound: A Systematic Review and Meta-analysis
Authors: Kamath Vandana , Chacko Mary Purna , Kirubakaran Richard , Mascarenhas Mariano , Kamath Mohan S. 
Published: October 2022

(Abstracted from Eur J Obstet Gynecol Reprod Biol 2022;271:235–244)
Chromosomal aberrations occur at a frequency of approximately 1 in 150 to 200 live births and are one of the most important causes of congenital anomalies and miscarriages. The introduction of chromosomal microarray (CMA) in the 1990s advanced the detection of chromosomal aberrations to include small copy number variants that previously were not detectable when only karyotype was used.
Mix and Mesh: An Electronic Database of FPMRS Mesh Products Through 2020
Authors: Woodburn Katherine L. , Cardenas-Trowers Olivia O. , Fitzgerald Jocelyn J. 
Published: October 2022

(Abstracted from Female Pelvic Med Reconstr Surg 2022;28:e154–e156)
In the United States, traditionally used treatment methods for pelvic organ prolapse (POP) and stress urinary incontinence include various types of synthetic mesh. Because the first transvaginal mesh product arrived on the market, over 1 million mesh products have been implanted.
Potential Role of the Corpus Luteum in Maternal Cardiovascular Adaptation to Pregnancy and Preeclampsia Risk
Authors: Conrad Kirk P. , von Versen-Höynck Frauke , Baker Valerie L. 
Published: October 2022

(Abstracted from Am J Obstet Gynecol 2022;226:683–699)
Preeclampsia (PE) affects 4.6% (95% confidence interval [CI], 2.7–8.2) of all pregnancies worldwide and often necessitates iatrogenic premature delivery, increasing the likelihood of neonatal morbidity and low birthweight. Mothers and their children who survive PE are at increased risk of developing adverse cardiovascular events later in life, although the mechanism behind this and many of the PE disease manifestations remains unclear.
Live-Birth Outcomes Among Women With Infertility and Anti-Müllerian Hormone Levels of 0.3 ng/mL or Lower
Authors: Romanski Phillip A. , Gelvin Brady , Bortoletto Pietro , Rosenwaks Zev , Kang Hey-Joo 
Published: October 2022

OBJECTIVE:
To estimate the live-birth rate per in vitro fertilization (IVF) cycle and after cumulative infertility treatment among patients with anti-müllerian hormone (AMH) levels of 0.3 ng/mL or lower.

METHODS:
We conducted a retrospective cohort study at a single academic center of patients with infertility and AMH levels of 0.3 ng/mL or lower who initiated one or more IVF cycles (2013–2019). Exclusion criteria included prior chemotherapy, hormonal contraceptive use within 3 months of AMH level measurement, and severe male factor infertility. Patients were stratified by Society for Assisted Reproductive Technology (SART) age group. The primary outcome was live-birth rate per IVF cycle. Live-birth outcomes were compared with the 2018 SART National Summary Report for live births per single intended oocyte retrieval, with proportion difference (PD) and 95% CI reported.

RESULTS:
A total of 978 patients were included. The median (interquartile range) number of cycles initiated was 2 (1–3). With the first initiated cycle, the live-birth rate for those with AMH levels of 0.3 ng/mL or lower was significantly lower in each age category compared with the SART live-birth rate per single initiated cycle (younger than 35 years: 26.2% vs 55.6%, PD 29.4%, 95% CI 20.9–37.9%; 35–37 years: 15.9% vs 40.8%, PD 24.9%, 95% CI 19.0–30.9%; 38–40 years: 12.6% vs 26.8%, PD 14.3%, 95% CI 10.2–18.3%; 41–42 years: 4.7% vs 13.4%, PD 8.7%, 95% CI 5.9–11.6%; older than 42 years: 1.2% vs 4.1%, PD 2.9%, 95% CI 1.5–4.3%). In patients aged 35–37, 38–40, 41–42, and older than 42 years, the cumulative live-birth rate after up to three initiated cycles was comparable with the SART live-birth rate per single initiated cycle but remained significantly lower in patients younger than age 35 years (PD 16.8%, 95% CI 7.3–26.2%). After all treatments were included (cumulative IVF, ovulation induction, and unassisted cycles), live-birth rates were similar to SART live-birth rates per single initiated cycle in all age groups.

CONCLUSION:
Compared with national outcomes, patients with AMH levels of 0.3 ng/mL or lower had a significantly lower chance of live birth after their first initiated cycle. However, the cumulative live-birth rate after up to three initiated cycles was comparable with national live-birth outcomes per single initiated cycle in patients aged 35 years or older. In patients younger than age 35 years, only when all IVF and non-IVF treatment cycles were included did the cumulative live-birth rate become comparable with the national rate per single IVF cycle.
Patterns in Contraceptive Use Among Women of Mexican Origin in the United States and in Mexico
Authors: Coleman-Minahan Kate , Boniface Emily R. , Fuentes-Rivera Evelyn , Saavedra-Avendaño Biani , Riosmena Fernando , Darney Blair G. 
Published: October 2022

OBJECTIVE:
To examine current contraceptive use by parity among four ethnicity and nativity groups: non-Latina White women in the United States, Mexican-American women in the United States, foreign-born women of Mexican origin in the United States, and Mexican women in Mexico.

METHODS:
We combined nationally representative data from sexually active women, aged 15–44 years, and not seeking pregnancy from the U.S. National Survey of Family Growth and the Mexican National Survey of Demographic Dynamics. This is a secondary binational analysis. Using multivariable logistic regression, we estimated the prevalence of moderately or most effective contraceptive method use (compared with least effective or no contraceptive method) by ethnicity and nativity and tested the interaction between ethnicity and nativity and parity.

RESULTS:
Compared with non-Latina White women, women of Mexican origin had lower odds of using a moderately or most effective contraceptive method (adjusted odds ratio [aOR] [95% CI] Mexican-American women: 0.69 [0.54–0.87]; foreign-born women: 0.67 [0.48–0.95]; Mexican women in Mexico: 0.59 [0.40–0.87]). Among parous women, the adjusted probability of using a moderately or most effective contraceptive method was approximately 65% among all four groups. Contraceptive method use did not differ by parity among non-Latina White women. However, parous Mexican-American women were 1.5 times more likely to use moderately or most effective contraceptive methods than nulliparous Mexican-American women (adjusted probability 66.1% vs 42.7%). Parous foreign-born women were 1.8 times more likely to use most or moderately effective contraceptive methods than their nulliparous counterparts (64.5% vs 36.0%), and parous Mexican women in Mexico were three times more likely to use moderately or most effective contraceptive methods (65.2% vs 21.5%).

CONCLUSION:
Findings suggest that access to effective contraception is limited outside the context of childbearing for women of Mexican origin in the United States and, to an even larger extent, in Mexico.
Association of Physician Densities and Gynecologic Cancer Outcomes in the United States
Authors: Smick Alexandra H. , Holbert Michael , Neff Robert 
Published: October 2022

OBJECTIVE:
To evaluate whether there is an association between county-level obstetrician–gynecologist (ob-gyn) and primary care physician (PCP) densities and gynecologic cancer outcomes in the United States.

METHODS:
A retrospective cohort study of gynecologic cancers (uterine, ovarian, and cervical) in the Surveillance, Epidemiology, and End Results (SEER) database was performed from 2005 to 2018. County-level demographics were abstracted from the SEER database, population density from the United States Census Bureau, and physician density (ob-gyns and PCPs/100,000 females) from the Area Health Resources File. Backward stepwise regression models were used.

RESULTS:
Final analysis included 113,938 patients for stage at diagnosis analysis and 98,573 patients for 5-year survival analysis. Uterine, ovarian, and cervical cancers represented 60.0%, 25.0%, and 15.0% of patients, respectively. Most counties (57%) were nonmetropolitan and had a mean ob-gyn density of 8 per 100,000 females and a mean PCP density of 89 per 100,000 females. Multivariate analysis showed that increasing PCP density was associated with earlier stage at diagnosis (95% CI −6.27 to −0.05; P<.05) and increased 5-year survival rates in cervical cancer (95% CI 0.03–0.09; P<.05). Obstetrician–gynecologist density was not found to affect stage or survival outcomes for uterine or ovarian cancer. Analysis of sociodemographic factors for cervical cancer showed that median household income was negatively correlated with stage (P=.01) and that the percentage of those with bachelor’s degrees and metropolitan status were positively correlated with 5-year survival rates (P<.01). For uterine cancer, the percentage of Black females was positively correlated with stage (P<.01) and negatively correlated with 5-year survival rates (P<.01).

CONCLUSION:
Increasing PCP density, but not ob-gyn density, is associated with earlier stage at diagnosis and improved 5-year survival rates in cervical cancer. County-level sociodemographic factors, including population diversity, metropolitan status, educational attainment, and household income, were also correlated with outcomes across all cancer types. Targeting PCP supply and education in lower density counties may improve population-based care for cervical cancer.
Preimplantation Genetic Testing for Aneuploidy With Comprehensive Chromosome Screening in Patients Undergoing In Vitro Fertilization
Authors: Cheng Xin , Zhang Yu , Deng Haidong , Feng Yuning , Chong Weelic , Hai Yang , Hao Pengfei , He Jialing , Li Tiangui , Peng Liyuan , Wang Peng , Xiao Yangchun , Fang Fang 
Published: October 2022

OBJECTIVE:
To review the effect of comprehensive chromosome screening–based preimplantation genetic testing for aneuploidy (PGT-A) in women undergoing in vitro fertilization (IVF) treatment, we conducted this meta-analysis to compare pregnancy outcomes of women who did and did not undergo such testing.

DATA SOURCES:
We searched Medline, EMBASE, the Cochrane Central Register of Controlled Trials, and ClinicalTrials.gov from their inception until February 28, 2022, for randomized controlled trials focusing on PGT-A treatment without any language restrictions.

METHODS OF STUDY SELECTION:
Randomized controlled trials involving women undergoing IVF with or without PGT-A and comprehensive chromosome testing. Pooled relative risks (RRs) with 95% CIs were calculated for the primary outcome using a random-effects model with the Mantel-Haenszel method.

RESULTS:
A total of nine trials with 3,334 participants were included. Overall, PGT-A was not associated with an increased live-birth rate (RR 1.13, 95% CI 0.96–1.34, I2=79%). However, PGT-A raised the live-birth rate in women of advanced maternal age (RR 1.34, 95% CI 1.02–1.77, I2=50%) but not in women of nonadvanced age (RR 0.94, 95% CI 0.89–0.99, I2=0%).

CONCLUSION:
Preimplantation genetic testing for aneuploidy increases the live-birth rate in women of advanced maternal age.

SYSTEMATIC REVIEW REGISTRATION:
PROSPERO, CRD42022311540.
Incarcerated Gravid Uterus Liberated by Placement of a Vaginal Balloon
Authors: Abelman Sarah H. , Jayakumaran Jenani S. , Sigdel Manisha , Baxter Jason K. 
Published: October 2022

BACKGROUND:
Incarceration of the gravid uterus is an infrequent obstetric complication that can cause severe complications.

CASE:
A 37-year-old woman, G3P2002, at 14 weeks of gestation presented with vomiting, back pain, and urinary retention. She was noted to be in acute renal failure, with a creatinine level of 9.24 mg/dL, and results of her physical examination were concerning for uterine incarceration. Passive maneuvers and manual pressure failed to resolve the incarceration. Under spinal anesthesia, a Bakri balloon was inflated in the posterior vagina, elevating the fundus and relieving the incarcerated uterus.

CONCLUSION:
An intravaginal balloon may aid in the alleviation of uterine incarceration.
Patterns of Risky Health Behaviors and Associations With Chronic Diseases Among Young Adult Nursing Students: A Latent Class Analysis
Authors: DONG Chaoqun , CHEN Hua , YANG Yeqin , LI Yi , SUN Yumei , SUN Hongyu 
Published: October 2022

ABSTRACT

Background
Little is known about how health behaviors cluster to form meaningful patterns that influence health outcomes in young adult nursing students.

Purpose
The purpose of this study was to identify the unique health behavior patterns among young adult nursing students in China and examine the associations between health behaviors and chronic diseases.

Methods
Using an electronic app, the achievements of an exercise target, sedentary behavior, smoking and drinking, and dietary patterns were assessed in 1,480 nursing student participants aged 18–24 years from two medical universities in Eastern China.

Results
A four-class model was developed using latent class analysis that included the “failure to achieve exercise target, alcohol-drinking, and insufficient fruit and vegetable group” (Group 1, n = 187, 12.6%), the “alcohol-drinking and sedentary behavior group” (Group 2, n = 290, 19.6%), the “sedentary behavior only group” (Group 3, n = 721, 48.7%), and the “failure to achieve exercise target only group” (Group 4, n = 282, 19.1%). Logistic regressions indicated that nursing students in Group 2 (odds ratio [OR] = 0.42), Group 3 (OR = 0.51), and Group 4 (OR = 0.30) were less likely to have chronic diseases than those in Group 1 after adjusting for sociodemographic variables.

Conclusions
The health behaviors were clustered in different patterns among young adult nursing students. Tailoring interventions to specific groups is suggested to improve health outcomes.
Examining the Needs-Based Time Use of Chinese Nursing Assistants: A Time–Motion Study
Authors: WANG Xinxia , SHEN Jun , CHEN Qiu 
Published: October 2022

ABSTRACT

Background
In light of the worldwide shortage of nurses and to maximize the effectiveness of the nursing care available, it is important to investigate the components of the care regularly provided by nursing assistants (NAs) to older adults. Well-organized allocation of NA care activities is directly linked to the quality of care provided to nursing home (NH) residents and their quality of life. However, relevant knowledge about the actual time allocation of NAs in this context is lacking, as previous related studies have focused only on the duration necessary for NAs to complete nursing activities.

Purpose
This study was designed to investigate the time allocation of NAs in completing job tasks necessary to meet the needs of residents in nursing care units at an NH in China.

Methods
A time–motion method and nonparticipatory observations were used to collect data. Two postgraduate students observed 15 NAs' time expenditure and nursing content simultaneously during day shifts for 3 weeks as the NAs provided care to residents in four different nursing care levels. Data on nursing time and activity frequency were collected using a worklist based on the Zuluaga–Raysmith model.

Results
The 119.6 hours of observation included 8,907 discrete observed activities. In terms of the care provided to meet the needs of residents, the most time-consuming activities were physical health care (26.8%) and communication care (18.3%), followed by mental, emotional, social, and spiritual health care (14.1%) and protection and security care (12.6%). The higher the level of care, the higher the proportion of somatic nursing time and nursing activity frequency. However, the time and frequency of psychological and spiritual care showed an opposite trend.

Conclusions
Because of their lack of formal nursing training and skills, NAs pay more attention to meeting the physiological needs of residents while ignoring their mental needs. Moreover, their effectiveness in providing spiritual care tends to be quite low. Furthermore, the NA nursing activity classification system based on the Zuluaga–Raysmith model developed in this study is applicable for designing nursing work tasks, organizing NH units, and improving the quality of life of residents, as this model accurately reflects the essence of NA work.
Effect of 0.12% Chlorhexidine Oral Rinse on Preventing Hospital-Acquired Pneumonia in Nonventilator Inpatients
Authors: CHEN Yen-Chin , KU En-Ni , TSAI Pei-Fang , NG Cheng-Man , WANG Jiun-Ling , LIN Che-Wei , KO Nai-Ying , CHANG Ying-Ju 
Published: October 2022

ABSTRACT

Background
Nonventilator hospital-associated pneumonia (NV-HAP) is a nosocomial infection with a multifactorial etiology that is particularly prevalent in individuals with poor oral health.

Purpose
This study was designed to determine the effect of a 0.12% chlorhexidine gluconate oral rinse intervention on oral health and on reducing NV-HAP in inpatients.

Methods
A randomized, double-blind, and triple-arm clinical trial was conducted on a sample of 103 patients aged ≥ 50 years. Using the blocking sample method, patients were randomly assigned into three groups. These included Group A, using an oral rinse solution of 0.12% chlorhexidine; Group B, using Listerine; and Group C, using a standard saline oral rinse. In addition to routine hospital-associated pneumonia preventative nursing care, the participants used the oral rinse solutions twice a day with a period of at least 9 hours between each use. Oral health, the degree of bacterial exposure, and the clinical pneumonia index scale were evaluated in each of the groups at baseline (first day), on Intervention Days 3 and 7, and at discharge. The clearance rate was calculated by dividing the number of bacteria cleared by the total frequency of oral bacteria in the collected culture × 100%.

Results
Each arm of the study was composed of 34–35 participants, with an average hospitalization duration of 7.5 days. There was no incidence of NV-HAP or any changes in clinical pulmonary infection score among the three groups. Group A achieved a more significant improvement in oral health assessment tool scores between baseline and discharge than either Group B or C (p = .03), particularly in the tongue, gums, and tissues; saliva; and oral cleanliness subscales. In addition, Group A reported higher clearance rates for Staphylococcus (100.00% vs. 66.67% vs. 66.67%, respectively), Escherichia coli (100.00% vs. 60.00% vs. 66.67%, respectively), and Pseudomonas aeruginosa (75.00% vs. 46.30% vs. 25.00%, respectively) than Groups B and C.

Conclusions/Implications for Practice
Although the results do not provide evidence supporting the use of a 0.12% chlorhexidine oral rinse as better in terms of preventing NV-HAP in middle-aged and elderly inpatients, nursing supervision was found to have an overall positive effect on oral health. The use of oral rinse with 0.12% chlorhexidine for nonventilated patients with poor oral health may be recommended.
Hand Function After an Electrical Accident—A Case-Control Study
Authors: Rådman Lisa , Wold Andrew , Norman Kerstin , Olausson Håkan , Thordstein Magnus 
Published: October 2022

Objective
Electrical accidents cause both acute and long-term injuries. The care of acute injuries is somewhat standardized, but currently recommendations or assessment tools are not available for assessing the long-term effects of an accident on hand function.

Methods
A case-control study of 24 healthy controls and 24 cases, 1 to 5 years after an electrical accident and with self-reported neurosensory symptoms, was performed using three hand-function tests: the Disabilities of the Arm, Shoulder, and Hand questionnaire, the Purdue Pegboard test, and the Shape and Texture Identification test.

Results
Compared with the control group, patients received statistically significantly lower scores for the Disabilities of the Arm, Shoulder, and Hand outcome measure and the Purdue Pegboard and for one finger on the Shape and Texture Identification test.

Conclusion
Hand function is affected after an electrical accident in individuals with self-reported neurosensory symptoms.
Voluntary Field Recall of Advanced Bionics HiRes Cochlear Implants: A Single-Institution Experience
Authors: McHugh Christopher I. , Swedenborg Britta K. , Chen Jenny X. , Jung David H. , Mankarious Leila A. , Quesnel Alicia M. , Cohen Michael S. , Arenberg Julie G. , Franck Kevin H. , Santos Felipe 
Published: October 2022

Objectives
In 2020, Advanced Bionics (AB) announced a recall of two cochlear implant (CI) models, the “HiRes Ultra” and “HiRes Ultra 3D”, because of reports of hearing degradation. The present study examines clinical parameters and patient features in cases of device failure and evaluates outcomes after reimplantation.

Materials and Methods
A series of 52 patients implanted with the recalled devices experienced suspected device failure and subsequently underwent revision CI placement at a tertiary academic medical center between December 2019 and November 2021.

Results
Consonant–nucleus–consonant scores and individual phonemes increased significantly between patients' preoperative evaluation and primary cochlear implantation. Performance declined significantly before revision and recovered after revision CI placement. Similarly, pure-tone average thresholds improved between preoperative and primary CI, fell before revision surgery, and were corrected with revision implantation. As a group, patients reached their peak hearing performance significantly faster after revision CI (mean ± standard deviation, 53.4 ± 51.8 d) compared with their primary CI (mean ± standard deviation, 260.6 ± 245.9 d). Electrical field imaging performed by AB and device impedance measurements were found to be abnormal in the basally positioned electrodes (electrodes 9–16).

Conclusion
Hearing performance degradation is significant in AB Ultra device failures and seems to be linked to the basal-most electrodes in the array. Revision outcomes have been robust, necessitating continued monitoring of affected patients and support for reimplantation procedures.

Level of Evidence
IV.