bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma Proteome Profiling of Patients With In-stent Restenosis by Tandem Mass Tag-Based Quantitative Proteomics Approach
Authors: Hou Jingyuan , Deng Qiaoting , Liu Sudong , Qiu Xiaohong , Deng Xunwei , Zhong Wei , Zhong Zhixiong 
Published: February 2022

BackgroundDespite the widespread application of new drug-eluting stents, a considerable portion of patients experience in-stent restenosis (ISR). To date, the pathophysiologic mechanisms of ISR remain poorly understood.MethodsIn this study, we collected plasma samples from ISR patients (n = 29) and non-ISR patients (n = 36) after drug-eluting stent implantation, as well as from healthy controls (HCs) (n = 32). Our goal was to investigate differences in plasma protein profiles using tandem mass tag (TMT) labeling coupled with liquid chromatography and tandem mass spectrometry. The proteomic data were validated by enzyme-linked immunosorbent assay (ELISA). Bioinformatic analyses were conducted to analyze potential pathways and protein-protein interaction (PPI) involved in ISR.ResultsA total of 1,696 proteins were identified, of which 278 differed in protein abundance between non-ISR and HCs, 497 between ISR and HCs, and 387 between ISR and non-ISR, respectively. Bioinformatic analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and PPI, further demonstrated that differentially abundant proteins between ISR and non-ISR are involved in several crucial biological processes and signaling pathways, such as focal adhesion, platelet activation, Rap1 signaling, regulation of actin cytoskeleton, and cholesterol metabolism. Among the identified differentially abundant proteins in ISR, 170 were increased in abundance relative to both non-ISR patients and HCs. Some of these proteins were identified to have critical functions for atherosclerosis development and might be involved in ISR pathology. Among these proteins, 3 proteins with increased abundance including fetuin-B, apolipoprotein C-III (APOC3), and cholesteryl ester transfer protein (CETP) were confirmed by ELISA.ConclusionsThis is the first study provided a comprehensive proteomic profile to understand ISR pathology, which may help identify early diagnostic biomarkers and therapeutic targets.
Regulation of Ion Channel Function in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Cancer Cell Secretion Through DNA Methylation
Authors: Zhong Rujia , Zhang Feng , Yang Zhen , Li Yingrui , Xu Qiang , Lan Huan , Lang Siegfried , Cyganek Lukas , Burgermeister Elke , El-Battrawy Ibrahim , Zhou Xiaobo , Akin Ibrahim , Borggrefe Martin 
Published: February 2022

BackgroundCardiac dysfunction including arrhythmias appear frequently in patients with cancers, which are expected to be caused mainly by cardiotoxic effects of chemotherapy. Experimental studies investigating the effects of cancer cell secretion without chemotherapy on ion channel function in human cardiomyocytes are still lacking.MethodsThe human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) generated from three healthy donors were treated with gastrointestinal (GI) cancer (AGS and SW480 cells) medium for 48 h. The qPCR, patch-clamp, western blotting, immunostaining, dot blotting, bisulfite sequence, and overexpression of the ten-eleven translocation (TET) enzyme were performed for the study.ResultsAfter treated with cancer cell secretion, the maximum depolarization velocity and the action potential amplitude were reduced, the action potential duration prolonged, peak Na+ current, and the transient outward current were decreased, late Na+ and the slowly activating delayed rectifier K+ current were increased. Changes of mRNA and protein level of respective channels were detected along with altered DNA methylation level in CpG island in the promoter regions of ion channel genes and increased protein levels of DNA methyltransferases. Phosphoinositide 3-kinase (PI3K) inhibitor attenuated and transforming growth factor-β (TGF-β) mimicked the effects of cancer cell secretion.ConclusionsGI cancer cell secretion could induce ion channel dysfunction, which may contribute to occurrence of arrhythmias in cancer patients. The ion channel dysfunction could result from DNA methylation of ion channel genes via activation of TGF-β/PI3K signaling. This study may provide new insights into pathogenesis of arrhythmia in cancer patients.
SAR Offset Tracking Based on Feature Points
Authors: Peng Lincai , Wang Hua , Ng Alex Hay-Man , Yang Xiaoge 
Published: February 2022

The offset tracking approach has been widely used to measure large ground deformation as a complement to Interferometric Synthetic Aperture Radar (InSAR) when its coherence is poor and/or the deformation gradient is large. The standard offset tracking procedures estimate deformation of tie points, which are uniformly distributed over two SAR images, resulting in many unsatisfactory measurements. In this paper, we propose a feature point offset tracking (FPOT) procedure to overcome the limitation of the standard method. First, we identify feature points using the Speeded Up Robust Feature (SURF) algorithm. Improper feature points are masked using external land coverage information like water coverages. Then, we use the standard cross-correlation algorithm to find offsets of the remaining feature points between reference and secondary images. The offset outliers are removed using a quadtree filtering. Finally, the resultant deformation field is generated by removing systematic offsets estimated with far-field feature points. We assess the effectiveness of our proposed procedure using the 2016 Mw 7.8 Kaikōura earthquake in New Zealand. In far-field where deformation is expected to be negligible, histograms of offset distribution show that the root-mean-square error (RMSE) is decreased from 0.07 pixels to 0.02–0.03 pixels for regular points and feature points, respectively, after quadtree filtering. The RMSE between our FPOT-derived offsets and GPS measurements are 0.14 and 0.48 m for range and azimuth offsets, respectively. The results show that our proposed procedure can significantly improve the efficiency, accuracy, and reliability with respect to the standard regular point offset tracking (RPOT).
Albedo Parametrizations for the Laohugou Glacier No.12 in the Qilian Mountains—Previous Models and an Alternative Approach
Authors: Wang Lihui , Zhang Dongwei , Steiner Jakob F. , He Xiaobo , Chen Jizu , Liu Yushuo , Li Yanzhao , Jin Zizhen , Qin Xiang 
Published: February 2022

Accurate estimates of albedo can be crucial for energy balance models of glaciers. A number of algorithms exist which are often site dependent and rely on accurate measurements or estimates of snow depth. Using the well-established COSIMA model we simulate the energy and mass balance of the Laohugou Glacier No.12 in the Qilian Mountains, on the northern fringe of the Qinghai-Tibetan Plateau, a glacier that has been well studied in the past. Using energy flux and mass balance measurements between 2010 and 2015 we were able to validate the model over multiple seasons. Using the original albedo parametrization, the model fails to reproduce the observed mass balance. We show that this is due to the failure to estimate snow depth accurately. We therefore applied two alternative albedo algorithms, one well established example and one new parametrization only dependent on temperature and time since last snow fall. As a result, mass balance simulations improve considerably from a RMSE of 0.53 m w.e. for the original parametrization to 0.39 and 0.19 m w.e. for the uncalibrated established and the new calibrated model respectively. Modelled albedo during the ablation period (NSE = 0.05, R2 = 0.33) is more accurate than during the accumulation period (NSE = −0.37, R2 = 0.04). Testing the new model at another glacier on the Tibetan Plateau shows that a local recalibration of parameters remains necessary to achieve satisfying results. Investigations into the effect of impurities in snow, regional moisture sources and changing surface characteristics with rising temperatures will be crucial for accurate projections into the future.
The Role of Sex Hormones on Bone Mineral Density, Marrow Adiposity, and Muscle Adiposity in Middle-Aged and Older Men
Authors: Xu Li , Zhao Qian , Li Kai , Zhang Yong , Wang Chao , Hind Karen , Wang Ling , Liu Yandong , Cheng Xiaoguang 
Published: February 2022

PurposeThe etiology of age-related bone loss is less clear in men. This study is aimed to observe the variations of endogenous sex hormone concentrations with increasing of age in men, and investigate their relations to bone mass, marrow adiposity, and muscle adiposity.MethodsA total of 199 community-dwelling Chinese men (aged 41 to 82 years) were included and measured of serum total estradiol, total testosterone, and follicle-stimulating hormone (FSH) concentrations by enzyme-linked immunosorbent assay (ELISA). Vertebral trabecular volumetric bone mineral density (vBMD) was measured by quantitative computed tomography for all participants, and vertebral marrow fat content and erector muscle fat content were quantified by Chemistry-shift-encoding magnetic resonance imaging in 62 participants.ResultsIn this population, FSH concentration increased (p &lt; 0.001) gradually with aging. Lower vBMD was independently associated with higher FSH concentration (β = -0.216, p &lt; 0.001), but not with total estradiol or total testosterone. For each standard deviation increase in FSH there was a 50% higher risk of an individual having osteopenia or osteoporosis (vBMD &lt; 120 mg/cm3). Marrow fat content and erector muscle fat content were greater in osteopenic and osteoporotic men, but there were no associations with sex hormones concentrations.ConclusionIn summary, FSH but not total estradiol or total testosterone is related to vertebral trabecular vBMD in middle-aged and older Chinese men. Neither marrow adiposity nor muscle adiposity is associated with sex hormones.
Potentials and Integrated Suitability Pre-assessment of Wastewater Treatment Plants as Local Energy Cells
Authors: Neugebauer Georg , Lichtenwoehrer Peter , Huber Franz , Stoeglehner Gernot , Kretschmer Florian 
Published: February 2022

In 2018 the European Union has recognized wastewater as a renewable energy source, particularly due to its thermal energy content. To create and further develop knowledge on the possible role that wastewater could play in the energy transition, this article pursues two objectives: 1) it presents an overview on the electric and thermal energy potentials available at the wastewater treatment plants (WWTPs) in the Central European area also taking their spatial context to adjacent settlement structures into account. 2) It introduces a small number of easy to apply criteria to (pre-)assess the suitability of WWTPs for being integrated into local energy (heat) supply concepts from an integrated perspective (considering energetic, spatial and environmental aspects). The investigations address the energy generation potentials of 3,315 WWTPs (with a treatment capacity of at least 5,000 population equivalents) in the area under survey. Analysis reveal, that notable amounts of heat ranging to almost 26 TWh/a are available. Their exploitation appears very promising, as investigations also show, that about 73% of the considered WWTPs are situated near or even within existing settlement structures implying very advantageous heat supply distances. Following the estimation of energy potentials, which is made available in the supplementary material, the presented suitability criteria can then be used to identify the most promising WWTPs in a specific regional/local context, in the investigated Central European area and beyond.
The Relationship Between Low-Density Lipoprotein Cholesterol and Progression of Mild Cognitive Impairment: The Influence of rs6859 in PVRL2
Authors: Xiao Qianyi , Xi Jianxiong , Wang Ruru , Zhao Qianhua , Liang Xiaoniu , Wu Wanqing , Zheng Li , Guo Qihao , Hong Zhen , Fu Hua , Ding Ding 
Published: February 2022

Background: Genome-wide association studies have identified many Alzheimer’s disease (AD) genetic-risk single nucleotide polymorphisms (SNPs) and indicated the important role of the cholesterol/lipid metabolism pathway in AD pathogenesis. This study aims to investigate the effects of cholesterol and genetic risk factors on progression of mild cognitive impairment (MCI) to AD.Methods: We prospectively followed 316 MCI participants aged ≥50 years with a baseline cholesterol profile and SNP genotyping data for 4.5 years on average in a sub-cohort of the Shanghai Aging Study. Total cholesterol, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol in serum were measured at baseline. SNP genotyping was performed using a MassARRAY system. At follow-up, consensus diagnosis of incident dementia and AD were established based on medical, neurological, and neuropsychological examinations. Cox regression models were used to assess the association of cholesterol and SNP with incident AD.Results: The AG/AA genotypes of PVRL2 rs6859 were significantly associated with increased incident AD in MCI participants, compared with GG genotype (adjusted hazard ratio [HR] 2.75, 95% confidence interval [CI] 1.32–5.76, p = .007, false discovery rate–adjusted p = .030). In PVRL2 rs6859 AG/AA carriers, each-1 mmol/L higher level of LDL-C was significantly associated with a 48% decreased risk of AD (adjusted HR 0.52, 95%CI 0.33–0.84, p = .007). Consistent results were obtained when using LDL-C as the categorical variable (P for trend = 0.016).Conclusion: The relationship between LDL-C and progression of MCI may be influenced by genetic variants.
Case Report: Microincision Vitreous Surgery Induces Bleb Failure in Eyes With Functional Filtering Bleb
Authors: Oogi Satomi , Nakakura Shunsuke , Asaoka Ryo , Terao Etsuko , Dote Saki , Matsuya Kanae , Kimura Yui 
Published: February 2022

PurposeTo investigate the effect of microincision vitreous surgery (MIVS) on intraocular pressure (IOP) control in glaucomatous eyes with functional filtering bleb.MethodsWe enrolled 18 patients (15 males; median age, 73 years) who previously had filtering surgery and underwent MIVS with functional filtering bleb. Kaplan–Meier method was used to calculate the survival rate with defined the failure as when more number of preoperative antiglaucoma medication was started or additional glaucoma surgery including bleb revisions were performed, and IOP increase of 20% (criteria 1) and 30% (criteria 2) from preoperative levels after 2 weeks of MIVS.ResultsThe median follow-up duration was 970 days. Preoperative IOP was 13.3 ± 3.8 mmHg (mean ± SD). Postoperative IOP were 14.7 ± 4.9 (P=0.365), 15.2 ± 3.5 (P=0.137), 16.4 ± 5.6 (P = 0.073), 17.6 ± 6.1(P = 0.020), and 14.5 ± 4.0 (P = 0.402) mmHg at 3, 6, 12, and 15 months and final visit, respectively (compared to preoperative IOP). The number of antiglaucoma medications was a median of 1.0 (range 0–4) preoperatively and 0 (0–4) at the final visit (P = 0.238). The survival rates were 55%/61% at 3 months, 50%/61% at 6 months, and 38%/55% at 12 months with criteria 1 and 2, respectively. Four eyes (22%) received additional glaucoma surgery during follow-up.ConclusionAfter several months of MIVS, IOP was likely to increase. We should focus on IOP control by conducting long-term follow-ups.
Brain Activity Changes in Slow 5 and Slow 4 Frequencies in Patients With Optic Neuritis: A Resting State Functional MRI Study
Authors: Yan Kai , Shi Wen-Qing , Su Ting , Liao Xu-Lin , Wu Shi-Nan , Li Qiu-Yu , Yu Jing , Shu Hui-Ye , Zhang Li-Juan , Pan Yi-Cong , Shao Yi 
Published: February 2022

ObjectiveWe used the amplitude of low-frequency fluctuation (ALFF) method to investigate spontaneous brain activity in patients with optic neuritis (ON) in specific frequency bands.Data and MethodsA sample of 21 patients with ON (13 female and eight male) and 21 healthy controls (HCs) underwent functional magnetic resonance imaging (fMRI) scans in the resting state. We analyzed the ALFF values at different frequencies (slow-4 band: 0.027–0.073 Hz; slow-5 band: 0.01–0.027 Hz) in ON patients and HCs.ResultsIn the slow-4 frequency range, compared with HCs, ON patients had apparently lower ALFF in the insula and the whack precuneus. In the slow-5 frequency range, ON patients showed significantly increased ALFF in the left parietal inferior and the left postcentral.ConclusionOur results suggest that ON may be involved in abnormal brain function and can provide a basis for clinical research.
Dose-Intensified Stereotactic Ablative Radiation for Localized Prostate Cancer
Authors: Chen Lily , Gannavarapu Bhavani S. , Desai Neil B. , Folkert Michael R. , Dohopolski Michael , Gao Ang , Ahn Chul , Cadeddu Jeffrey , Bagrodia Aditya , Woldu Solomon , Raj Ganesh V. , Roehrborn Claus , Lotan Yair , Timmerman Robert D. , Garant Aurelie , Hannan Raquibul 
Published: February 2022

PurposeStereotactic ablative radiation (SAbR) has been increasingly used in prostate cancer (PCa) given its convenience and cost efficacy. Optimal doses remain poorly defined with limited prospective comparative trials and long-term safety/efficacy data at higher dose levels. We analyzed toxicity and outcomes for SAbR in men with localized PCa at escalated 45 Gy in 5 fractions.Methods and MaterialsThis study retrospectively analyzed men from 2015 to 2019 with PCa who received linear-accelerator-based SAbR to 45 Gy in 5 fractions, along with perirectal hydrogel spacer, fiducial placement, and MRI-based planning. Disease control outcomes were calculated from end of treatment. Minimally important difference (MID) assessing patient-reported quality of life was defined as greater than a one-half standard deviation increase in American Urological Association (AUA) symptom score after SAbR.ResultsTwo-hundred and forty-nine (249) low-, intermediate-, and high-risk PCa patients with median follow-up of 14.9 months for clinical toxicity were included. Acute urinary grade II toxicity occurred in 20.4% of patients. Acute grade II GI toxicity occurred in 7.3% of patients. For follow-up &gt; 2 years (n = 69), late GU and GI grade ≥III toxicity occurred in 5.8% and 1.5% of patients, respectively. MID was evident in 31.8%, 23.4%, 35.8%, 37.0%, 33.3%, and 26.7% of patients at 3, 6, 12, 24, 36, and 48 months, respectively. The median follow-up for biochemical recurrence was 22.6 months with biochemical failure-free survival of 100% at 1 year (n = 226) and 98.7% for years 2 (n = 113) and 3 (n = 54).ConclusionsSAbR for PCa at 45 Gy in 5 fractions shows an encouraging safety profile. Prospective studies with longer follow-up are warranted to establish this dose regimen as standard of care for PCa.
Perioperative Serum Scoring Systems Predict Early Recurrence and Poor Prognosis of Resectable Pancreatic Cancer
Authors: Li Shengnan , Zhang Gengpu , Lu Yang , Zhao Tiansuo , Gao Chuntao , Liu Weishuai , Piao Yongjun , Chen Yanan , Huang Chongbiao , Chang Antao , Hao Jihui 
Published: February 2022

ObjectiveSome patients with pancreatic ductal adenocarcinoma (PDAC) are prone to rapid recurrence or metastasis after radical resection. However, evaluation methods for effectively identifying these patients are lacking. In this study, we established perioperative serum scoring systems to screen patients with early recurrence and poor prognosis.MethodsWe systematically analysed 44 perioperative serum parameters, including systemic inflammatory parameters, coagulation system parameters, tumor markers, and 18 clinicopathological characteristics of 218 patients with radical resection in our centre. Univariate Cox regression and LASSO regression models were used to screen variables. Kaplan-Meier survival analysis was used to compare relapse-free survival and overall survival. Multivariate Cox regression was used to evaluate the independent risk variables. AUC and C-index were used to reveal the effectiveness of the models. In addition, the effectiveness was also verified in an independent cohort of 109 patients.ResultsPreoperative systemic immune coagulation cascade (SICC) (including increased neutrophil to lymphocyte ratio, decreased lymphocyte to monocyte ratio, increased platelet and fibrinogen) and increased postoperative tumor markers (TMs) (CA199, CEA and CA242) were independent risk factors for early recurrence of resectable pancreatic cancer. On this basis, we established the preoperative SICC score and postoperative TMs score models. The patients with higher preoperative SICC or postoperative TMs score were more likely to have early relapse and worse prognosis. The nomogram based on preoperative SICC, postoperative TMs, CACI, smoking index, vascular cancer embolus and adjuvant chemotherapy can effectively evaluate the recurrence rate (AUC1 year: 0.763, AUC2 year: 0.679, AUC3 year: 0.657) and overall survival rate (AUC1 year: 0.770, AUC3 year: 0.804, AUC5 year: 0.763).ConclusionPreoperative SICC and postoperative TMs can help identify resectable PDAC patients with early recurrence and poor prognosis.
Inadequate Bioavailability of Intramuscular Epinephrine in a Neonatal Asphyxia Model
Authors: Berkelhamer Sara K. , Vali Payam , Nair Jayasree , Gugino Sylvia , Helman Justin , Koenigsknecht Carmon , Nielsen Lori , Lakshminrusimha Satyan 
Published: February 2022

BackgroundOver half a million newborn deaths are attributed to intrapartum related events annually, the majority of which occur in low resource settings. While progress has been made in reducing the burden of asphyxia, novel approaches may need to be considered to further decrease rates of newborn mortality. Administration of intravenous, intraosseous or endotracheal epinephrine is recommended by the Newborn Resuscitation Program (NRP) with sustained bradycardia at birth. However, delivery by these routes requires both advanced skills and specialized equipment. Intramuscular (IM) epinephrine may represent a simple, low cost and highly accessible alternative for consideration in the care of infants compromised at birth. At present, the bioavailability of IM epinephrine in asphyxia remains unclear.MethodsFour term fetal lambs were delivered by cesarean section and asphyxiated by umbilical cord occlusion with resuscitation after 5 min of asystole. IM epinephrine (0.1 mg/kg) was administered intradeltoid after 1 min of positive pressure ventilation with 30 s of chest compressions. Serial blood samples were obtained for determination of plasma epinephrine concentrations by ELISA.ResultsEpinephrine concentrations failed to increase following administration via IM injection. Delayed absorption was observed after return of spontaneous circulation (ROSC) in half of the studies.ConclusionsInadequate absorption of epinephrine occurs with IM administration during asphyxial cardiac arrest, implying this route would be ineffective in infants who are severely compromised at birth. Late absorption following ROSC raises concerns for risks of side effects. However, the bioavailability and efficacy of intramuscular epinephrine in less profound asphyxia may warrant further evaluation.