bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ovicidal toxicity of plant essential oils and their major constituents against two mosquito vectors and their non-target aquatic predators
Authors: Moungthipmalai Tanapoom , Puwanard Cheepchanok , Aungtikun Jirapon , Sittichok Sirawut , Soonwera Mayura 
Published: November 2022

Abstract

Plant essential oil (EO) is a natural alternative to synthetic chemical insecticides for mosquito control. EOs from Citrus aurantium L., Cymbopogon citratus (Stapf.), and Cinnamomum verum (J. Presl.) were selected for topical assay of their ovicidal activity against Aedes aegypti L. and Aedes albopictus (Skuse). Their efficacy was compared to that of 1% (w/w) temephos. In addition, their non-toxicity against aquatic mosquito predators, Poecilia latipinna and Poecilia reticulate, was tested. Found by GC-MS analysis, the major constituent of C. verum EO was trans- cinnamaldehyde, of C. aurantium EO was d-limonene, and of C. citratus EO was geranial. Both C. verum EO and trans-cinnamaldehyde at a high concentration (30,000 ppm) exhibited high ovicidal activity against Ae. aegypti and Ae. albopictus eggs after 48 h of incubation with an inhibition rate of 91.0–93.0% for C. verum EO and 96.7–95.2% for trans-cinnamaldehyde. The combination of C. verum EO + geranial exhibited the strongest synergistic inhibition activity (100%) against the two mosquito vectors and was five times more effective than temephos. Moreover, they were not toxic to the non-target fishes. As a safe ovicidal agent for mosquito egg control, the combination of C. verum EO + geranial has excellent potential.
Hydrogen-bonded structure and optical nonlinearities in the proton-transfer complex of 8-hydroxy-5-nitroquinoline with ρ-toluenesulfonic acid
Authors: Sivaraman Shanmugam , Markkandan R , Pandiarajan Kasinathan , Meenakshisundaram Subbiah 
Published: November 2022

Abstract
Single crystals of 8-hydroxy-5-nitroquinolinium p-toluene sulfonate (HNT) were grown by the slow evaporation solution growth technique. The structure was elucidated by single crystal X-ray diffraction analysis and the crystal belongs to the monoclinic system with the space group C2/c. The crystallinity of HNT was studied by powder X-ray diffraction analysis. The presence of functional groups was determined by FT-IR spectral analysis. The band gap energy is estimated by the application of the Kubelka–Munk algorithm. The charge transfer characteristic of the compound was studied by frontier molecular orbitals (FMOs) analysis. The first-order hyperpolarizability of the HNT molecule was found to be 285.45 × 10− 30 esu, which is ~ 750 times higher compared to the reference urea molecule. Investigation of the intermolecular interactions and crystal studies packing via Hirshfeld surface analysis, based on single-crystal XRD, reveals that the close contacts are associated with molecular interactions. Fingerprint plots of the Hirshfeld surfaces were used to locate and analyze the percentage of hydrogen-bonding interactions. The Mulliken charge of the present molecule was theoretically analyzed. The Kurtz-Perry powder technique has been used to estimate the second harmonic generation. Observed small SHG and large hyperpolarizability are rationalized.
Exploring the delay in the informed consent procedures of patients with ST-segment elevation myocardial infarction undergoing percutaneous coronary intervention: Is it worth the wait?
Authors: Maimaitiming Mailikezhati , Ma Junxiong , Dong Xuejie , Zhou Shuduo , Li Na , Zhang Zheng , Lu Shijuan , Chen Lianglong , Ma Likun , Yu Bo , Ma Yitong , Zhao Xingsheng , Zheng Zhaofen , Shi Hong , Zheng Zhi-Jie , Jin Yinzi , Huo Yong 
Published: November 2022

Abstract
Objective:To investigated the factors associated with informed consent delay in patients with ST-segment elevation myocardial infarction (STEMI) undergoing percutaneous coronary intervention (PCI) and the association between the delay and door-to-balloon time.
Methods:We conducted a nationally representative retrospective cohort study using patient data reported by hospital-based chest pain centers from 1 January 2016 to 31 December 2020.
Results: In total, 257,510 patients were enrolled in the analysis. Mean informed consent delay time was 22.4 minutes, accounting for 39.3% in door-to-balloon time. Older age (≥65 years) was significantly correlated with informed consent delay time (RR: 1.034, p=0.001). Compared with ethnic Han patients, the minority (RR: 1.146, p<0.001) had more likelihood to extend consent giving; compared with patients who were single, longer informed consent time was found in married patients (RR: 1.054, p=0.006). Patients with intermittent chest pain (RR: 1.034, p=0.011), and chest pain relief (RR: 1.085, p=0.005)were more likely to delay informed consent. As for transfer modes, EMS (RR: 1.063, p<0.001), transfer-in (RR: 1.820, p<0.001), and in-hospital onset (RR: 1.099, p=0.002) all had positive correlations with informed consent delay time compared to walk-in. Informed consent delay was significantly associated with prolonged door-to-balloon time (OR: 1.002, p<0.001).
Conclusion: Informed consent delay provokes prolongation of emergency treatment delay, which potentially leads to poor outcome of STEMI patients. It is essential to shorten the delay time by identifying and intervening modifiable factors that are associated with shortening the informed consent procedure in China and other countries.
Simulatenous evaluation of composting experiments and metagenome analyses to illuminate the effect of Streptomyces spp. on organic matter degradation
Authors: Kocak Fadime Ozdemir , Tanır Saadet Gizem Ertekin , Cetin Ayten Kumas , Degirmenci Levent 
Published: November 2022

Abstract
Streptomyces spp. were isolated via sampling during thermophilic phase of a compost system. Isolates were subjected to a careful and detailed sets of procedures in order to select best candidates effective on organic matter degradation. This effect, on organic matter degradation was illumanated by evaluation of composting experiments and metagenome analyses in accordance. Results indicated dominanace of strains when added to compost systems. This dominanace was found to be partially due to possible secondary metabolite production of isolates determined by antagonism tests. The dominance of strains was in fact distinctive in establishment of variant organic matter degradation. Enzymatic activity as a result of strains’ dominance was provided as an explanation based on evaluation of metagenomic counts. Metagenome count numbers revealed the tendency of compost microbiota towards degradation products of cellulose and xylan was also determined to be least favored polysaccharide. Results also implied a decrease of competition between the dominant strain and microbita members in the case of sequential cellulose and xylan degradation. Meticulous evaluation of results obtained from metagenome analysis also provided some insights on certain conditions regarding the progress of composting along with storage conditions of manure prior to use.
Layer-dependent stability of 2D mica nanosheets
Authors: Kim Jae-Hun , Kulish Vadym. V. , Wu Shunnian , Wu Ping , Osada Minoru , Kim Hyoun Woo , Kim Sang Sub 
Published: November 2022

Abstract
We report on the layer-dependent stability of muscovite-type two-dimensional (2D) mica nanosheets (KAl3Si3O10(OH)2). First-principles calculations on mica nanosheets with different layer thicknesses (n = 1, 2, and 3) reveal their layer-dependent stability; odd-numbered 2D mica nanosheets are more stable than even-numbered ones, and the preferable stability of odd-numbered layers originates from electronic effects. A core-shielding model is proposed with a reasonable assumption, successfully proving the instability of the even-numbered mica nanosheets. Raman imaging supports that the population of odd-numbered mica nanosheets is predominant in exfoliated mica products. The alternating charge states with odd/even layers were evidenced by Kelvin probe force microscopy. We also demonstrate a unique photocatalytic degradation, opening new doors for environmental applications of mica nanosheets.
Analysis of the reduction processes at the bottom of Lake Meirama
Authors: Juncosa Ricardo , Delgado Jorge , cereijo Jose Luis , Muñoz Andrea 
Published: November 2022

Abstract
The formation of natural lakes is a process that takes place over thousands of years, although the volumetric formation depends on hydrological and climatological phenomena, reaching a stationary hydraulic regime, the evolution of hydrochemistry is more complex and obeys not only phenomena of stoichiometry and chemical kinetics, but also diffusion processes. Depending on the depth of the lakes, the anoxisation process originating from the bottom is the first phase of the lake's methanogenesis. For this, the course of many thousands of years is necessary, so the studies carried out in the lakes are limited to the current knowledge of the state in which they are, without being able to have real information in this process of methanogenesis. There are no data available on the generation process of a natural lake in its primary stages. In this case, taking advantage of the rehabilitation of the old open-pit mining of Meirama (Northwest Spain), consisting of the controlled flooding of the hole by groundwater, by stopping the perimeter pumping, and the derivation of the nearby streams, whose contribution was the majority with respect to the subterranean contribution, there has been the opportunity to physically and chemically monitor the complete filling of said hole. The present study focuses on the analysis of the evolution of the different processes initiated in the methanogenesis of the lake bottom identified in the well-known Redox ladder: obtaining oxygen from the reduction of nitrogenous compounds and metallic oxides, from the reduction of the sulfate and the generation of methane from carbon compounds, the latter phase without reaching. Although the methanization process is very slow, we have had the opportunity to know the formation of a lake at its origin, from the hydrochemical point of view.
Primary yolk sac tumor of the endometrium combined with total visceral inversion: A case report and literature review
Authors: Liu Rong , Wang Yanru , Wang Yani , Wang Xinfeng , Chen Xiujie , Hu Jiangong 
Published: November 2022

Abstract

Background Yolk sac tumor (YST) is a highly malignant germ cell tumor, a majority of which originate from the gonads and is extremely rare from endometrium. Case presentation Here we present a case of a 42-year-old woman suffered from primary pure yolk sac tumor of endometrium complicated with total visceral inversion. The patient presented at our hospital with irregular vaginal bleeding, imageological examination showed a space-occupying lesion in the cervix and serum Alpha-fetoprotein (AFP) level was significantly high (more than 1210ng/ml). Then she underwent total hysterectomy, bilateral adnexectomy and pelvic lymph node dissection. Subsequent postoperative pathological diagnosis was yolk sac tumor arising from endometrium. Next, the patient was treated with 6 cycles of chemotherapy with Pingyangmycin, etoposide and cisplatin regimen and was alive without evidence of recurrence or distant metastases for 13 months. Conclusions This rare disease needs to be differentiated from endometrial epithelial neoplasia and the significant increase in AFP is helpful for diagnosis. Combined with previous literature reports, comprehensive staging laparotomy or maximum cytoreductive surgery complemented by standard chemotherapy can usually achieve a good efficacy.
Diversity analysis and gene function prediction of bacteria of Alxa white cashmere goat milk and its dairy products in Inner Mongolia
Authors: Yang Xiaofeng , Bao wuyundalai , He Yuxing , Yu Jinghe , Liu Mingchao , Bao Yiran 
Published: November 2022

Abstract
At present, there are few reports on bacterial diversity of Alxa white cashmere goat milk and its dairy products.The results of this study showed that there were significant differences in bacterial diversity and abundance of Alxa white cashmere goat milk and its dairy products (p < 0.05), The dominant bacteria phylum and dominant bacteria genus are changed when goat milk is made into traditional dairy products. In this study, we found that there were many unspecified bacterial genera in goat milk and its dairy products, which need to be further studied. In addition, the relative abundance of foodborne pathogenic bacteria generic such as Enterobacter, Enterococcus, Raoultella and Pseudomonas in dairy products decreased sharply, which provided a reference for the safety evaluation of goat milk and its dairy products. Prediction of bacterial gene function by PICRUSt2 software revealed that the main bacterial metabolic pathways in goat milk and dairy products were Global and overview maps, Carbohydrate metabolism and Amino acid metabolism, etc. This study shows that there are differences in the functions of metabolism-related genes in goat milk and dairy products, which further indicates that the functions of these major metabolic genes are closely related to probiotics in traditional dairy products. In summary, the results of this study not only have a certain understanding of the bacterial diversity in Alxa white cashmere goat milk and its dairy products, but also provide a reference for the study of probiotics, functional genes and safety evaluation in goat milk and its dairy products.
Explainable predictions of a machine learning model to forecast the postoperative length of stay for severe patients
Authors: Cho Ha Na , Ahn Imjin , Gwon Hansle , Kang Hee Jun , Kim Yunha , Seo Hyeram , Choi Heejung , Kim Minkyoung , Han Jiye , Kee Gaeun , Jun Tae Joon , Kim Young-Hak 
Published: November 2022

Abstract
Understanding the length of stay of severe patients who require general anesthesia is key to enhancing health outcomes. Here, we aim to discover how machine learning can support resource allocation management and decision-making resulting from the length of stay prediction. A retrospective cohort study was conducted from January 2018 to October 2020. A total cohort of 240,000 patients’ medical records was collected. The data were collected exclusively for preoperative variables to accurately analyze the predictive factors impacting the postoperative length of stay. The main outcome of this study is an analysis of the length of stay (in days) after surgery until discharge. The prediction was performed with ridge regression, random forest, XGBoost, and multi-layer perceptron neural network models. The XGBoost resulted in the best performance with an average error within 3 days. Moreover, we explain each feature’s contribution over the XGBoost model and further display distinct predictors affecting the overall prediction outcome at the patient level. The risk factors that most importantly contributed to the stay after surgery were as follows: a direct bilirubin laboratory test, department change, calcium chloride medication, gender, and diagnosis with the removal of other organs. Our results suggest that healthcare providers take into account the risk factors such as the laboratory blood test, distributing patients, and the medication prescribed prior to the surgery. We successfully predicted the length of stay after surgery and provide explainable models with supporting analyses. In summary, we demonstrate the interpretation with the XGBoost model presenting insights on preoperative features and defining higher risk predictors to the length of stay outcome. Our development in explainable models supports the current in-depth knowledge for the future length of stay prediction on electronic medical records that aids the decision-making and facilitation of the operation department.
IL-33 Involved in the Progression of Liver Fibrosis Regulated by ICOS/ICOSL Signaling in Early Stage of Mice Schistosomiasis
Authors: Liu Lei , Wang Peng , Xie Shi-Qi , Pu Wen-Jie , Xu Jing , Xia Chanoming 
Published: November 2022

Abstract
Backgrounds:
IL-33 is an important pro-inflammatory factor involved in the schistosomiasis liver fibrosis. The inducible costimulator (ICOS) could bind to ICOS ligand (ICOSL), participating in chronic schistosomiasis.
Methods
The levels of IL-33 were determined in ICOSL-KO and WT mice infected with Schistosoma japonicum (S. japonicum), respectively. Then recombinant IL-33 (rIL-33) was injected into ICOSL-KO mice infected with S. japonicum.
Results
The concentrations of IL-33 were lower in ICOSL-KO mice that in WT mice. Furthermore, the injection of rIL-33 successfully aggravated liver fibrosis in ICOSL-KO mice, increased the numbers of lymphocyte antigen 6C (Ly6C)hi, enhanced the expression of C-C chemokine ligand (CCL)2, CCL5 and C-X-C motif chemokine 2 (CXCL2), and promoted polarization of T helper (Th) cells to Th2 cells, as well as induced the autophagy and apoptosis of hepatic stellate cells (HSCs).
Conclusions
Overall, the liver fibrosis was aggravated in ICOSL-KO mice along with the rIL-33, which could skew the polarization of Mφ, induce Th cells activation, HSCs apoptosis and autophagy through Smad2/3 and TGF-β signaling pathway. Our study gives an insight into antagonizing IL-33 as a potential target against liver fibrosis in ICOSL-KO mice.
Maraging Steel Powder Recycling Effect on the Tensile and Fatigue Behavior of Parts Produced Through the Laser Powder Bed Fusion (L-PBF) Process
Authors: RAYAN OTHMANE , Brousseau Jean , Belzile Claude , Ouafi Abderrazak El 
Published: November 2022

Abstract

Additive manufacturing (AM) has advanced the manufacturing industry and has been employed in a wide range of industrial applications, including in aerospace, automotive, medical and die-casting equipment. To ensure the cost-effectiveness of the AM process, unfused powder must be recycled even if its characteristics may change after each cycle, making essential the validation of powder quality and component mechanical performances. Despite the research published to date, predicting the mechanical performance of printed parts issued from reused powder remains challenging since it is dependent on many AM process variables. Until now, no research has looked at the impact of powder recycling on the fatigue behavior of maraging steel components. This study investigates the impact of maraging steel powder reuse on powder characteristics, as well as on the tensile and fatigue properties of printed components. Our results indicate that the powder particle size distribution increased after eight powder recycling, particle morphology showed the presence of aggregates, broken particles, shattered and deformed particles, while powder apparent density remained constant. Powder reusing had no significant impact on the surface roughness of as-built specimens. Although there was a slight decrease in mechanical properties over reuse cycles, tensile and fatigue performance remained globally stable, while the standard deviation of fatigue stress became narrower after eight cycles. Finally, fractography revealed that the fatigue fracture surfaces of components manufactured from an eight-time recycled powder have more fusion defects and carbon inclusions than the parts made from virgin powder.
Assessment of the dibenzothiophene desulfurization potential of indigenously isolated bacterial consortium IQMJ-5: A different approach to safeguard the environment
Authors: Khan Javed , Ali Muhammad Ishtiaq , Jamal Asif , Achakzai Jahangir Khan , Shirazi Jafir Hussain , Haleem Abdul 
Published: November 2022

Abstract
Biodesulfurization is emerging as a valuable technology for the desulfurization of dibenzothiophene (DBT) and its alkylated substitutes, which are otherwise regarded as refractory to other physical and chemical desulfurizing techniques. However, the technique is currently facing the issue of the nonavailability of an effective microbial isolate with the capability of increased desulfurizing rate and the ability to tackle the problem of product inhibition. Pure cultures belonging to different genera have been used at a large scale for the desulfurization of fossil fuels while studies on the desulfurization of fossil fuels employing microbial consortia are entirely missing. Herein, we isolated several kinds of dibenzothiophene desulfurizing bacterial consortia from hydrocarbon-contaminated soil samples by conventional enrichment technique. The outcomes of Gibb’s assay analysis showed that six isolates followed the “4S” pathway and converted DBT to 2-hydroxybiphenyl (2-HBP). Among the isolates, I5 showed maximum growth rate (0.0064 g dry cell weight L-1h-1) and desulfurization activity (about 77% as indicated by HPLC analysis) and was considered for further in-depth experimentation. The analysis of 16S rRNA by high throughput sequencing approach of the I5 isolate revealed five types of bacterial phyla including Proteobacteria, Bacteroidetes, Firmicutes, Patascibacteria, and Actinobacteria (in order of abundance). The isolate showed significant tolerance to the inhibitory effect of both 2-HBP and sulfate and maintained growth in the presence of even about 1.0 mM initial concentration of both products. This clearly suggests that the isolate can be an efficient candidate for improving the quality of the coal.
Simultaneous determination of four gingerol components in ginger extracts by a single marker (QAMS) using a new calculation of relative correction factor
Authors: Li Zhining , Li Feifei , Li Xiao , Ma Yanni , Zhang Lixian , Fan Yi , Song Mengjiao , Zhang Taotao , Wei Yue 
Published: November 2022

Abstract
The lack of standard substances is a bottleneck for quality control in traditional and alternative medicine. Development of the ultra high-performance liquid chromatography coupled with triple quadrupole liquid-mass tandem system (UPLC-MS/MS) approaches combined with single standard for quantitative analysis of multi-component system (QAMS) allowed determination of several gingerol components in ginger extracts by implementation of the relative response factors (RRFs). Four gingerol components of 6-gingerol, 8-gingerol, 10-gingerol and 6-Shogaol were selected as analytes to evaluate the quality by UPLC-MS/MS. A new relative response factors (nRRF, fox) were established by calculating the ratio of the logarithm of the response and the injection concentration of the analytes to the internal reference. The contents of four analytes in ginger extracts were determined by the single marker of nonivamide used as the internal reference which did not exist in the sample and was similar to gingerol in structure and performance. The method was evaluated by a comparison of the quantitative results between the external standard method (ESM) and QAMS. The results showed that no significant differences were found in the quantitative results of the four contents of essential oil extract of ginger from three different extraction methods determined by ESM and QAMS. The QAMS method could serve as an accurate and convenient method in determining the gingerol components in the essential oil extract of ginger and its processed products.