bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interdental plaque reduction after the use of different devices in patients with periodontitis and interdental recession: A randomized clinical trial
Authors: Gennai Stefano , Nisi Marco , Perić Marina , Marhl Urska , Izzetti Rossana , Tonelli Matteo , Petrini Morena , Graziani Filippo 
Published: January 2022

AbstractObjectivesTo determine the efficacy of four different oral hygiene protocols involving adjunctive interdental devices in terms of plaque and bleeding scores in periodontitis‐affected patients.Material and methodsThis was a randomized clinical trial with a 5‐week follow‐up, including previously treated periodontitis patients not undergoing any supportive care. Patients were randomly allocated to 4 groups: manual toothbrush (group TB); toothbrush plus dental floss (group TB/F); toothbrush plus interdental brushes (group TB/IDB) and toothbrush plus rubber interdental picks (group TB/RIDB). Oral hygiene instructions (OHI) were provided at baseline (T‐7) and at T0 (1‐week) then bi‐weekly (T14/T28). At T0, supragingival professional cleaning was delivered. Plaque and bleeding indexes were taken at all timepoints by a single calibrated examiner, blind to group allocation.ResultsAt T‐7, patients were comparable for age, body mass index (BMI) and all clinical parameters, exceptions for the Interdental Angulated Bleeding Index. At T28, significant reductions of plaque and gingival inflammation were noted in all groups (p < 0.001) and within the expected ranges. Intergroup comparisons identified that group TB/IDB and group TB/RIDB achieved lower levels of plaque and inflammation than group TB (p < 0.05). Group TB/IDB and group TB/RIDB showed lower levels of plaque and lower inflammation as measured by angulated bleeding index than group TB/RIDB (p < 0.05).ConclusionInterdental brushes and rubber interdental picks were more efficient than toothbrushing alone and toothbrushing and floss in reducing plaque and gingival inflammation measured in a periodontitis‐affected population.
A branch‐and‐price approach to the multitasking scheduling with batch control on parallel machines
Authors: Gao Jinsheng , Zhu Xiaomin , Zhang Runtong 
Published: January 2022

AbstractThis paper addresses multitasking scheduling with batch control on parallel machines. The specific scenario can be found in the maintenance activities of skilled workers. The objective is to minimize the total weighted completion time. In the problem, each machine needs to be allocated at least to one batch of jobs. On each machine, the processing of a primary job may be interrupted by the waiting jobs of the same machine, which can be described as the multitasking scheduling. The existing algorithms and mathematical formulations of such a scheduling problem are not suitable for the problem. An exact branch‐and‐price algorithm with heuristic pricing strategy is devised. To fast find the minimized reduced cost of the pricing problem, an artificial bee colony algorithm is developed. Specifically, it is aimed at obtaining a partitioning schedule with a minimized reduced cost, which involves the allocation of batches and the multitasking scheduling of jobs. A weak enumeration operator is proposed to cope with the batch allocation. The computational results demonstrate that the branch‐and‐price algorithm with heuristic pricing strategy outperforms general‐purpose pure CPLEX solver by orders of magnitude.
Enhanced nuclear localization of YAP1‐2 contributes to EGF‐induced EMT in NSCLC
Authors: Guo Qiang , Quan Mei‐Yu , Xu Le , Cai Yaxin , Cai Jue‐Ting , Li Xue , Feng Guifeng , Chen Aiping , Yang Weiwei , Dhlamini Qhaweni , Jiang Tian‐Fang , Shen Chengguo , Chen Chengshui , Zhang Jin‐San 
Published: January 2022

AbstractYAP1, a key mediator of the Hippo pathway, plays an important role in tumorigenesis. Alternative splicing of human YAP1 mRNA results in two major isoforms: YAP1‐1, which contains a single WW domain, and YAP1‐2, which contains two WW domains, respectively. We here investigated the functions and the underlying regulatory mechanisms of the two YAP1 isoforms in the context of EGF‐induced epithelial‐mesenchymal transition (EMT) in non‐small cell lung cancer (NSCLC). Human NSCLC cell lines express both YAP1‐1 and YAP1‐2 isoforms—although when compared to YAP1‐1, YAP1‐2 mRNA levels are higher while its protein expression levels are lower. EGF treatment significantly promoted YAP1 expression as well as EMT process in NSCLCs, whereas EGF‐induced EMT phenotype was significantly alleviated upon YAP1 knockdown. Under normal culture condition, YAP1‐1 stable expression cells exhibited a stronger migration ability than YAP1‐2 expressing cells. However, upon EGF treatment, YAP1‐2 stable cells showed more robust migration than YAP1‐1 expressing cells. The protein stability and nuclear localization of YAP1‐2 were preferentially enhanced with EGF treatment. Moreover, EGF‐induced EMT and YAP1‐2 activity were suppressed by inhibitor of AKT. Our results suggest that YAP1‐2 is the main isoform that is functionally relevant in promoting EGF‐induced EMT and ultimately NSCLC progression.
Development and characterization of edible films made with Indian jujube fruit puree and pectin
Authors: Neog Biswajyoti , Das Jitul K. , Vijayakumar Akhila , Badwaik Laxmikant S. 
Published: January 2022

AbstractIndian jujube (Ziziphus mauritiana Lamk) fruit also known as “Ber” has been a useful fruit in India from age‐old days. It contains antioxidant, anti‐inflammatory, and antibacterial properties, additionally they are rich in vitamin C, thiamine, riboflavin, etc. The jujube puree was used for developing edible films along with varying concentrations of pectin (1, 2, 3, 4, and 5%). The edible films on characterization show that a rise in pectin concentration helps to improve the tensile strength of the film from 54.55 to 472.32 kPa. But the stability or water tolerance of the films were negatively affected by a rise in pectin concentration. The solubility of films was increased to 83.32% from 76.77%. Properties like color, water activity, and water vapor permeability were less affected with the addition of pectin. The work leads to the successful development of jujube puree—pectin‐based edible film that can create a positive influence on film mechanical and barrier properties. The properties of these edible films can be further improved with the administration of suitable cross‐linking agents or bioactive compounds.Practical ApplicationsIndian jujube is a seasonal fruit and generally available for a very short duration mostly from January to April and mostly consumed as raw. The pulp and peel of this fruit are rich in different bioactive compounds. As the fruit is highly perishable major fraction of the total yield gets wasted every year. It is not yet commercially explored to make value‐added products. The development of edible films from these fruit purees helps to introduce novel colors and flavors to the edible film research. The film composition used in the study can also be adapted for coating perishable fruits by spraying, which helps to add barriers to its spoilage. It can also help to increase the nutrition and quality of the product for which the film is used. The study helped to understand the potential of jujube fruit as a novel edible packaging material and opened new opportunities to utilize the indigenous varieties of seasonal fruits that are not celebrated in the market.
Morphological and molecular studies of a rare mucoralean species causing flower rot in Hylocereus polyrhizus
Authors: Ruangwong On‐Uma , Kunasakdakul Kaewalin , Wonglom Prisana , Dy Kim Sreang , Sunpapao Anurag 
Published: January 2022

AbstractThe order Mucorales typically includes fast‐growing saprotrophic and plant pathogenic fungi. Flower rot of red‐fleshed dragon fruit (Hylocereus polyrhizus) caused by Mucor‐like fungi was observed in a field in Phatthalung province, southern Thailand. Agglomeration of sporangiophores and sporangia on the wet rotten tissues of the red‐fleshed dragon fruit flower was detected. Fungal isolates obtained from symptomatic tissues were cultured on potato dextrose agar for macroscopic and microscopic observations. Based on the growth rate and morphology of sporangia and sporangiospores, the fungus was identified as Gilbertella persicaria, which was further confirmed using phylogenetic analyses of the internal transcribed spacer and nuclear large subunit ribosomal DNA sequences. Pathogenicity tests revealed that the fungal isolate G. persicaria caused flower rot, similar to that observed in the field. This study is the first report of flower rot disease in H. polyrhizus caused by G. persicaria in Thailand.
Epidemiology of heart disease in English Bull Terriers and echocardiographic characteristics of mitral valve abnormalities
Authors: Chompoosan C. , Schrøder A. S. , Höllmer M. , Bach M. B. T. , Møgelvang R. , Willesen J. L. , Langhorn R. , Koch J. 
Published: January 2022

ObjectivesTo present the prevalence and distribution of heart disease as well as echocardiographic findings in English Bull Terriers.Materials and MethodsOne hundred and one English Bull Terriers were retrospectively included to evaluate the prevalence and distribution of heart disease. Secondly, a retrospective study on mitral valve abnormalities was performed on three groups: a control group (n=120, 19 breeds) used to establish reference intervals for mean transmitral gradient; a healthy English Bull Terriers group (n=25) and an English Bull Terriers group with mitral valve abnormalities (n= 18). Healthy English Bull Terriers for which mitral inflow parameters were not obtainable and English Bull Terriers with other types of heart disease were excluded.ResultsThe prevalence of heart disease in English Bull Terriers was 65% (66/101), with mitral valve abnormalities (47%, 47/101) and aortic stenosis (29%, 29/101) being most common. The cut‐off value for normal mean transmitral gradient was 3.5 mmHg in the control group. The mean transmitral gradient for healthy English Bull Terriers was higher than for other dog breeds. Healthy English Bull Terriers had a smaller mitral valve area and mitral annulus diameter compared with dogs with a similar body surface area. A high heart rate, smaller mitral valve area, mitral regurgitation, and volume overload are associated with increased mean transmitral gradient in English Bull Terriers with mitral valve abnormalities.Clinical SignificanceWe suggest that mitral valve area, mitral annulus diameter and mean transmitral gradient measurements should be included in the echocardiographic protocol for English Bull Terriers.
Biological characteristics and whole-genome analysis of the potential probiotic, Lactobacillus reuteri S5
Authors: Shi Shuiqin , Dong J. , Cheng X. , Hu J. , Liu Y. , He G. , Zhang J. , Yu H. , Jia L. , Zhou D. 
Published: January 2022

Abstract
Lactic acid bacteria are micro-organisms used for probiotic purposes and form major parts of human and mammalian intestinal microbiota, exerting important health-promoting effects on the host. Here, we evaluated Lactobacillus reuteri strain S5 isolated from the intestines of healthy white feather broilers. Lactobacillus reuteri S5 grew best after 20 h of incubation in MRS medium. Lactic acid production was 1·42 mmol l−1 at 24 h, which was well tolerated. Activities of T-AOC, GSH-Px and T-SOD in the cell-free fermentation supernatant of L. reuteri S5 were higher than those in the bacteria, and the strain showed good hydrophobicity in vitro. The dominant carbon and nitrogen sources of L. reuteri S5 were glucose and soybean meal. A high-quality complete genome map of L. reuteri S5 was obtained using a Pacbio nanopore third-generation sequencing platform. The results showed that L. reuteri S5 possesses a complete primary metabolic pathway, encoding the main functional enzymes of the glycolysis pathway and pentose phosphate pathway. The genome contains genes encoding antioxidants and conferring tolerance to inorganic salt ions, acids and bile salts. This study shows that L. reuteri S5 is a probiotic strain with excellent probiotic characteristics and has great potential for the development of feed additives to promote animal health.
Implementing the Becoming Breastfeeding Friendly initiative in Scotland
Authors: McFadden Alison , Kendall Sally , Eida Tamsyn 
Published: January 2022

AbstractDespite strong policy support in Scotland, United Kingdom, key challenges to scaling up promotion, protection and support for breastfeeding remain. These include low breastfeeding rates and socioeconomic and regional inequalities. The Becoming Breastfeeding‐Friendly (BBF) process was implemented to highlight actions that could address these challenges. The Scottish BBF committee employed an iterative process of documentary analysis and evidence reviews supplemented by 18 interviews with key informants. The data were mapped to BBF benchmarks and each gear was scored accordingly. Nineteen draft recommendations addressing policy and practice gaps were prioritised. Ten recommendations were grouped into eight themes, which cross‐cut the BBF gears. The process took place from May 2018 to May 2019. The overall BBF Index score for Scotland was 2.4 indicating a strong scaling‐up environment for breastfeeding. Five gears were assessed as strong gear strength, and the remaining three were judged as moderate gear strength. Three recommendation themes illuminate strengths and areas for development. The theme ‘reinforcing political will’ showed effective leadership, strong policies and significant investment in supporting breastfeeding and highlights actions to sustain this. The theme ‘strengthening and coordinating breastfeeding messages’ revealed a need for coordination between government, health services and the third sector. The theme ‘promoting a supportive return to work environment’ highlighted that, while employment legislation is not devolved to the Scottish government, action could be taken by employers to optimise an enabling environment for breastfeeding. The BBF process identified strengths and triggered actions to enhance breastfeeding promotion, protection and support in Scotland.
IncHI1A plasmids potentially facilitate horizontal flow of antibiotic resistance genes to pathogens in microbial communities of urban residential sewage
Authors: Olesen Asmus K. , Pinilla‐Redondo Rafael , Hansen Mads F. , Russel Jakob , Dechesne Arnaud , Smets Barth F. , Madsen Jonas S. , Nesme Joseph , Sørensen Søren J. 
Published: January 2022

AbstractHorizontal gene transfer via plasmids is important for the dissemination of antibiotic resistance genes among medically relevant pathogens. Specifically, the transfer of IncHI1A plasmids is believed to facilitate the spread of antibiotic resistance genes, such as carbapenemases, within the clinically important family Enterobacteriaceae. The microbial community of urban wastewater treatment plants has been shown to be highly permissive towards conjugal transfer of IncP1 plasmids. Here, we tracked the transfer of the P1 plasmid pB10 and the clinically relevant HI1A plasmid R27 in the microbial communities present in urban residential sewage entering full‐scale wastewater treatment plants. We found that both plasmids readily transferred to these communities and that strains in the sewage were able to further disseminate them. Furthermore, R27 has a broad potential host range, but a low host divergence. Interestingly, although the majority of R27 transfer events were to members of Enterobacteriaceae, we found a subset of transfer events to other families, even other phyla. This indicates that HI1A plasmids facilitate horizontal gene transfer both within Enterobacteriaceae, but also across families of, in particular, Gammaproteobacteria, such as Moraxellaceae, Pseudomonadaceae and Shewanellaceae. pB10 displayed a similar potential host range to R27. In contrast to R27, pB10 had a high host divergence. By culture enrichment of the transconjugant communities, we show that sewage strains of Enterobacteriaceae and Aeromonadaceae can stably maintain R27 and pB10, respectively. Our results suggest that dissemination in the urban residual water system of HI1A plasmids may result in an accelerated acquisition of antibiotic resistance genes among pathogens.
Coupling of plant and mycorrhizal fungal diversity: its occurrence, relevance, and possible implications under global change
Authors: Fei Songlin , Kivlin Stephanie N. , Domke Grant M. , Jo Insu , LaRue Elizabeth A. , Phillips Richard P. 
Published: January 2022

SummaryFirst principles predict that diversity at one trophic level often begets diversity at other levels, suggesting plant and mycorrhizal fungal diversity should be coupled. Local‐scale studies have shown positive coupling between the two, but the association is less consistent when extended to larger spatial and temporal scales. These inconsistencies are likely due to divergent relationships of different mycorrhizal fungal guilds to plant diversity, scale dependency, and a lack of coordinated sampling efforts. Given that mycorrhizal fungi play a central role in plant productivity and nutrient cycling, as well as ecosystem responses to global change, an improved understanding of the coupling between plant and mycorrhizal fungal diversity across scales will reduce uncertainties in predicting the ecosystem consequences of species gains and losses.
Matrilineal empowers wheat pollen with haploid induction potency by triggering postmitosis reactive oxygen species activity
Authors: Sun Guoliang , Geng Shuaifeng , Zhang Hongjie , Jia Meiling , Wang Zhenyu , Deng Zhongyin , Tao Shu , Liao Ruyi , Wang Fang , Kong Xingchen , Fu Mingxue , Liu Shaoshuai , Li Aili , Mao Long 
Published: January 2022

Summary

Reactive oxygen species (ROS) play important roles during anther and pollen development. DNA damage may cause chromosome fragmentation that is considered to underlie chromosome elimination for haploid induction by matrilineal pollen, a key step in MATRILINEAL‐based double haploid breeding technology. But when and how DNA damage occurs is unknown.

We performed comparative studies of wheat pollens from the wild‐type and the CRISPR/Cas9 edited matrilineal mutant (mMTL).

Chemical assays detected a second wave of ROS in mMTL pollen at the three‐nuclei‐stage and subsequently, along with reduced antioxidant enzyme activities. RNA‐seq analysis revealed disturbed expression of genes for fatty acid biosynthesis and ROS homoeostasis. Gas chromatography‐mass spectrometry measurement identified abnormal fatty acid metabolism that may contribute to defective mMTL pollen walls as observed using electron microscopy, consistent with the function of MTL as a phospholipase. Moreover, DNA damage was identified using TdT‐mediated dUTP nick‐end labelling and quantified using comet assays. Velocity patterns showed that ROS increments preceded that of DNA damage over the course of pollen maturation.

Our work hypothesises that mMTL‐triggered later‐stage‐specific ROS causes DNA damage that may contribute to chromosome fragmentation and hence chromosome elimination during haploid induction. These findings may provide more ways to accelerate double haploid‐based plant breeding.
Septal flash correction with His‐Purkinje pacing predicts echocardiographic response in resynchronization therapy
Authors: Pujol‐López Margarida , Jiménez Arjona Rafael , Guasch Eduard , Doltra Adelina , Borràs Roger , Roca Luque Ivo , Castel María Ángeles , Garre Paz , Ferró Elisenda , Niebla Mireia , Carro Esther , Arbelo Elena , Sitges Marta , Tolosana José M. , Mont Lluís 
Published: January 2022

AbstractBackgroundHis‐Purkinje conduction system pacing (HPCSP) has been proposed as an alternative to Cardiac Resynchronization Therapy (CRT); however, predictors of echocardiographic response have not been described in this population. Septal flash (SF), a fast contraction and relaxation of the septum, is a marker of intraventricular dyssynchrony.MethodsThe study aimed to analyze whether HPCSP corrects SF in patients with CRT indication, and if correction of SF predicts echocardiographic response. This retrospective analysis of prospectively collected data included 30 patients. Left ventricular ejection fraction (LVEF) was measured with echocardiography at baseline and at 6‐month follow‐up. Echocardiographic response was defined as increase in five points in LVEF.ResultsHPCSP shortened QRS duration by 48 ± 21 ms and SF was significantly decreased (baseline 3.6 ± 2.2 mm vs. HPCSP 1.5 ± 1.5 mm p < .0001). At 6‐month follow‐up, mean LVEF improvement was 8.6% ± 8.7% and 64% of patients were responders. There was a significant correlation between SF correction and increased LVEF (r = .61, p = .004). A correction of ≥1.5 mm (baseline SF – paced SF) had a sensitivity of 81% and 80% specificity to predict echocardiographic response (area under the curve 0.856, p = .019).ConclusionHPCSP improves intraventricular dyssynchrony and results in 64% echocardiographic responders at 6‐month follow‐up. Dyssynchrony improvement with SF correction may predict echocardiographic response at 6‐month follow‐up.
Identification and estimation of threshold matrix‐variate factor models
Authors: Liu Xialu , Chen Elynn Y. 
Published: January 2022

AbstractMotivated by the growing availability of complex time series observed in real applications, we propose a threshold matrix‐variate factor model, which simultaneously addresses the sample‐wise and time‐wise complexities of a time series. The sample‐wise complexity is characterized by modeling matrix‐variate observations directly, while the time‐wise complexity is modeled by a threshold variable to describe the nonlinearity in time series. The estimators for loading spaces and threshold values are introduced and their asymptotic properties are investigated. Our matrix‐variate models compress data more efficiently than traditional vectorization‐based models. Furthermore, we greatly extend the scope of current research on threshold factor models by removing several restrictive assumptions, including existence of only one threshold, fixed factor dimensions across different regimes, and stationarity within regime. Under the relaxed assumptions, the proposed estimators are consistent even when the numbers of factors are overestimated. Simulated and real examples are presented to illustrate the proposed methods.