bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality of sleep and Factors Associated with Sleep Pattern Disturbance among Patients in Critical Care Units: Mixed Method study
Authors: kalal Nipin , Kumar Ashok , Rana Nimarta , kothari Nikhil 
Published: August 2022

AbstractBackground Sleep disturbances are amongst one of the most common problems reported for critically ill patients admitted to ICU. Sleep disturbances have a negative impact on physiological parameters of the patients, which may delay their recovery, raise health care cost, and increases the length of hospital stay. Methods A mixed method (quantitative and qualitative) study was conducted with the use of a convergent parallel research design. The study was conducted in critical care units and 72 patients were included by the nested sampling technique. Two tools were used for data collection; a Modified Sleep disturbing scale and Semi-structured questionnaire with an in-depth interview on patient’s experiences regarding the quality of sleep. Result It showed that the most considerable factors which affect sleep were the pain (1.27 ± 0.61), the routine care before sleep (1.31 ± 1.03), alarms (1.29 ± 0.70) followed by lighting (1.15 ± 0.59). The environmental factors were found significantly associated with participants age. Further, marital status was found significantly associated with patient’s factors (0.002*), staff (0.009*), and environmental factors (0.001*). Whereas, exceptionally there was no remarkable association established between these factors and participant’s gender, education, or previous history of addiction to sedative or hypnotic drugs. Conclusion Sleep disturbance is a major problem identified among hospitalized patients. Majority of patients who experience disturbance in sleep pattern and quality of life is ascribed to pain, routine care, alarms and lighting. Education regarding different strategies could be considered as an important aspect for uninterrupted sleep pattern and also aids the patient to get better sleep.
Comparison of Telebehavioral Health Outcomes from Rural Populations Across America
Authors: McCord Carly , Ullrich Fred , Merchant Kimberly , Bhagianadh Divya , Nelson EveLynn , Marcin James , Law Kari Beth , Neufeld Jonathan , Giovanetti Annaleis , Ward Marcia , Carter Knute 
Published: August 2022

Abstract
Background
This study investigates outcomes from two federal grant programs: the Evidence-Based Tele-Behavioral Health Network Program (EB THNP) funded from September 2018 to August 2021 and the Substance Abuse Treatment Telehealth Network Grant Program (SAT TNGP) funded from September 2017 to August 2020. As part of the health services implementation program, the aims of this study were to evaluate outcomes in patient symptoms of depression and anxiety across the programs’ 17 grantees and 95 associated sites, with each program having data from telehealth patients and from an in-person comparison group. The evaluation compares outcomes between the group of patients seen in-person and the group of patients seen viatelehealth.
Methods
The study design is a nonrandomized convenience sample across telehealth and in-person groups from sites with similar rural characteristics and during the same time period. Patient characteristics were to be collected at treatment initiation, and clinical outcome measures were to be collected at baseline and monthly on patients where clinically appropriate. The validated clinical outcome measure instruments included the Patient Health Questionnaire-9 (PHQ-9) for depression symptoms and the Generalized Anxiety Disorder-7 (GAD-7) scale for anxiety-related symptoms.
Results
Across a total of 1,514 patients, one-month change scores were improved indicating that PHQ-9 and GAD-7 scores decreased from baseline to the one month follow-up at similar rates in both the in-person and telehealth groups. Reduction in scores averaged 2.8 for the telehealth treatment group and 2.9 for the in-person treatment group in the PHQ-9 subsample and 2.0 for the telehealth treatment group and 2.4 for the in-person treatment group in the GAD-7 subsample. There was no statistically significant association between the modality of care (telehealth treatment group versus in-person comparison group) and the one-month change scores for either PHQ-9 or GAD-7. Individuals with higher baseline scores demonstrated the greatest decrease in scores for both measures. Upon adjusting for baseline scores and clustering within grantee program, patient demographics were not found to be significantly associated with change in anxiety or depression symptoms.
Conclusions
In our very large pragmatic study comparing behavioral health treatment delivered to a population of patients in rural, underserved communities, we found no clinical or statistical differences in improvements in depression or anxiety symptoms as measured by the PHQ-9 and GAD-7 between patients treated via telehealth or in-person.
Robotic radical distal gastrectomy for advanced gastric cancer using the soft coagulation scissors technique
Authors: Yamashita Yoshito , Tatsubayashi Taichi , Okumura Koichi , Sakura Yusuke , Miyamoto Takumi 
Published: August 2022

Abstract
We developed a new technique for safe and precise lymph node dissection in robotic gastrectomy for gastric cancer using monopolar curved scissors with soft coagulation, called the “soft coagulation scissors technique.” Its technical details are as follows: a long bipolar grasper in the first arm and monopolar curved scissors in the third arm are mainly used for D2 lymph node dissection, maximizing robotic arm articulation usage. The monopolar curved scissors were energized in the soft coagulation mode of the Valleylab™ FT10 energy platform (group A; n = 29) or in the forced coagulation mode with an effect1/power limit of 15 W of the ERBE VIO®dV (group B; n = 9), which was confirmed to be equivalent to the soft coagulation mode, and the tissue was dissected with scissors while being energized. Large blood vessels were precoagulated with a long bipolar grasper and dissected with monopolar curved scissors. Therefore, a wide surgical field was developed using Cadiere forceps in the fourth arm and an internal organ retractor, enabling quick precoagulation with the relatively free long bipolar grasper. Thirty-seven patients with advanced gastric cancer underwent robotic distal gastrectomy using this technique. There was no difference in surgical outcomes between group A and group B. The median volume of estimated blood loss was 10 g, and none of the patients had intra-abdominal infections, including pancreatic fistula. The soft coagulation scissors technique enables safe and precise lymph node dissection in robotic distal gastrectomy for advanced gastric cancer and could be a standard surgical technique for robotic gastrectomy for gastric cancer.
Spatio-temporal variations and drought of spring maize in Northeast China since 2002
Authors: Ji Lin , Wu Yongfeng , Ma Juncheng , Song Chenxi , Zhu Zhicheng , Zhao Aiping 
Published: August 2022

Abstract
A lot of maize is grown in Northeast China (Liaoning, Jilin, and Heilongjiang), however, this area is highly susceptible to drought. NDVI, LSWI, LST, and TVDI datasets from 2002 to 2020 were studied using the 8-day surface reflectance (SR) and land surface temperature (LST) of MODIS in this study. Spring maize distribution data were extracted using a decision tree classification method to reveal spatio-temporal patterns. The occurrences of mild, moderate, and severe droughts were investigated under spatio-temporal variations. The overall accuracy of verifying the spring maize distribution in 2018–2020 was above 85%. The stable, fluctuating, and low-frequency planting areas of spring maize accounted for 11.86%, 17.41%, and 34.86% of the study area, respectively. In the ‘Liandaowan’ region of Northeast China, the government directed to reduce the planting area in 2015. Distribution variations were characterized by continuous growth in the pre-adjustment stage (2002–2014), adjustment and reduction during the in-adjustment stage (2015–2017), and optimization and recovery in the post-adjustment stage (2018–2020). Compared with the fluctuating and low-frequency planting areas, moderate and severe droughts were higher in stable planting areas, accounting for 33.62% and 19.83%, respectively. There were more droughts in the pre-adjustment stage in the expanded planting area with a gradual decrease in the latter two stages. This rapid and large-scale monitoring of spatio-temporal variations and drought of spring maize lays the foundation for improved strategies to maintain field area and improve grain yield. This method could be easily applied to the study of other areas and could be combined with high-resolution and hyperspectral satellite data to improve monitoring accuracy.
In-situ stability assessment and remediation mechanism of typical antimony (Sb) tailings by combined organic-inorganic solid waste in Southwest China
Authors: Yang Chengya , Han Zhiwei , Luo Guangfei , He Yinpin 
Published: August 2022

Abstract
To explore the feasibility of reusing solid waste to stabilize high-content antimony ore arsenic (As) and antimony (Sb) tailings, red mud, fly ash, dried sludge, ferrous sulfate (FeSO4), and rice husk ash were used as the stabilizer. Different combinations of stabilizers were used to stabilize the Sb tailings in Qinglong and Dushan. The morphological transformation and leaching characteristics of As and Sb in the treated tailing samples were determined. The results showed that the stabilizer was beneficial for adjusting the pH of the tailings and increasing the organic matter content. The combined treatment with 5% red mud, 10% fly ash, 5% dried sludge, 1% FeSO4, and 1% rice husk ash had the best stabilization effects on As and Sb. The best stabilization efficiencies of As and Sb in Dushan were 86.97% and 79.11%, respectively, and in Qinglong were 87.42% and 70.36%, respectively. The leaching concentrations of As and Sb decreased considerably under neutral conditions, promoting the transformation of As and Sb from exchangeable, oxidizable, and reducible states to the residue state. Furthermore, “Correlation heat maps” and X-ray diffraction (XRD) analyses were undertaken to evaluate the best performance of the stabilized samples, which is related to (1) the adsorption and precipitation mechanism of iron-aluminum (hydroxide) compounds in red mud and fly ash; (2) complexation and chelation of the rich organic matter; (3) the main hydration products calcium silicate hydrate (C-S-H), the As/Sb precipitation process and (4) the mechanism of pH inhibition by FeSO4.
Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO
Authors: Dykstra James C. , Oort Jelle van , Yazdi Ali Tafazoli , Vossen Eric , Patinios Constantinos , Oost John van der , Sousa Diana Z. , Kengen Servé W. M. 
Published: August 2022

Abstract
Background
Ethyl acetate is a bulk chemical which is traditionally produced via energy intensive chemical esterification. Microbial production of this compound offers promise as a more sustainable alternative. So far, efforts have focused on using sugar-based feedstocks for microbial ester production, but extension to one-carbon substrates, such as CO and CO2/H2, is desirable. Acetogens such as Clostridium autoethanogenum present a promising microbial platform for biochemical production from one-carbon substrates.
Results
We engineered C. autoethanogenum to produce ethyl acetate from CO by heterologous expression of an alcohol acetyltransferase (AAT) that catalyzes the formation of ethyl acetate from acetyl-CoA and ethanol. Two AATs, Eat1 and Atf1 were expressed in C. autoethanogenum, and ethyl acetate was successfully produced by strains expressing Atf1. Production of ethyl acetate reached 0.2 mM when grown on CO. Supplementation of ethanol was investigated as potential boost for ethyl acetate production but resulted only in a 1.5-fold increase (0.3 mM ethyl acetate). Besides ethyl acetate, C. autoethanogenum expressing Atf1 could produce 4.5 mM of butyl acetate, when butanol was supplemented to the growth medium.
Conclusions
This work offers for the first time a proof-of-principle that autotrophic short chain ester production from C1 carbon feedstocks is possible and offers leads on how this approach can be optimized in the future.
Nano-Metals Forming Bacteria in Egypt. II. Efficacy towards Biomolecules, Ultrastructure, Growth Parameters, and Eco-Friendly Therapeutic of Soft Rot/Blackleg Genera
Authors: Shoeib Alia A. , Ashmawy Nader A. , Kamal Ayman , Zaki Sahar Abd El Fatah 
Published: August 2022

Abstract
The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in isolated DNA and decreased proteins and carbohydrates concentration compared with untreated cells. Using Scanning Electron Microscope (SEM), the treated cells showed collapsed and small pits in the cell wall. Using Transmission Electron Microscope (TEM), internal changes showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation of vacuoles, and condensation of cytoplasm.
Disease severity ex vivo of potato tuber infected with tested genera demonstrated that NPs treatment didn't show any rotted tissue compared with untreated. The ability to uptake and accumulate FeNPs from the soil in potato (Solanum tuberosum) seedlings; Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used. ICP-OES recorded an increase in iron content of treated potato (Solanum tuberosum) seedlings with NPs, compared with untreated. FeNPs can be used to control soft rot/blackleg diseases; instead of copper pesticides. It could be a new, and alternative approach to traditional disease management methods and increase the plant's nutritional value.
Chilling and Heat requirements for woody taxa in Tétouan (NW Morocco)
Authors: Boullayali Asmaa , Galán Carmen , Martínez-Bracero Moisés , Bouziane Hassan 
Published: August 2022

Abstract

Impact of climate change affects chilling and heat accumulation that phenological development of plant needs, air temperature being determinant for flowering, pollination, and fruit production; but few knowledges are available in the winter and spring climate of Tétouan. The physiological mechanisms of the reproductive cycle that control pollen emission are related with temperature, and different environmental factors regulate strongly the reproductive phases of the plant’s life cycle. However, from an empirical point of view, the alternance between endodormancy and ecodormancy is not well detectable. Our work focuses on determining the heat accumulation periods associated to the thermal balance needed to fulfill the chilling and heat required for budbreak and bloom timing. To evaluate Morus, Pistacia, Quercus and Olea response to changes in chill and heat accumulation rates, the Dynamic Model and the Growing Degree Days Method were used. Regression analyses identified the chilling and forcing periods for these taxa. Over the past 12 years, chill accumulation during tree dormancy increased significantly for most of woody taxa studied, while heat accumulation decreased not significantly for all taxa. Heat accumulation was the main driver of bloom timing, with effects of variation in chill accumulation.
Multi-focus light-field microscopy for high-speed large-volume imaging
Authors: Zhang Yi , Wang Yuling , Wang Mingrui , Guo Yuduo , Li Xinyang , Chen Yifan , Lu Zhi , Wu Jiamin , Ji Xiangyang , Dai Qionghai 
Published: August 2022

Abstract
High-speed visualization of three-dimensional (3D) processes across a large field of view with cellular resolution is essential for understanding living systems. Light-field microscopy (LFM) has emerged as a powerful tool for fast volumetric imaging. However, one inherent limitation of LFM is that the achievable lateral resolution degrades rapidly with the increase of the distance from the focal plane, which hinders the applications in observing thick samples. Here, we propose Spherical-Aberration-assisted scanning LFM (SAsLFM), a hardware-modification-free method that modulates the phase-space point-spread-functions (PSFs) to extend the effective high-resolution range along the z-axis by ~ 3 times. By transferring the foci to different depths, we take full advantage of the redundant light-field data to preserve finer details over an extended depth range and reduce artifacts near the original focal plane. Experiments on a USAF-resolution chart and zebrafish vasculatures were conducted to verify the effectiveness of the method. We further investigated the capability of SAsLFM in dynamic samples by imaging large-scale calcium transients in the mouse brain, tracking freely-moving jellyfish, and recording the development of Drosophila embryos. In addition, combined with deep-learning approaches, we accelerated the three-dimensional reconstruction of SAsLFM by three orders of magnitude. Our method is compatible with various phase-space imaging techniques without increasing system complexity and can facilitate high-speed large-scale volumetric imaging in thick samples.
Seroprevalence and Associated Risk Factors of Peste des Petits Ruminants Among Ovine and Caprine in Selected Districts of Afar Region, Ethiopia
Authors: Dubie Teshager , Dagnew Betelhem , Gelo Esrael , Negash Wossene , Hussein Fentaw , Woldehana Mulatu 
Published: August 2022

Abstract
Background: Peste des petits ruminant (PPR) is an acute, highly contagious and economically important transboundary viral disease of small ruminants. Despite the fact that FAO and OIE plan to eradicate the disease by 2030, some studies indicated an increasing seropositivity of the PPR infection in sheep and goats in Ethiopia. A cross-sectional study was employed to estimate the seroprevalence of PPR and to assess risk factors during the study period, February to April, 2020. A total of 384 serum samples were collected from apparently healthy sheep and goats. Competitive Enzyme Linked Immunosorbent Assay was used to detect the presence of antibodies against PPR at national veterinary institute. Descriptive statistics, Pearson’s chi‑square (X2) and logistic regression analysis were used is this study.Results: The overall animal level seroprevalence of PPR virus was found to be 60.15% (n=231/384) and species level prevalence rate was found to be 38.18% (n= 42) in sheep and 68.98% (n= 189) in goats in the study areas. Among the associated risk factors considered; species, sex, age and herd sizes were significantly associated (P<0.05) with PPR disease occurrence. Among the associated risk factors considered in this study, species, sex, age and herd size were found to be statistically associated with the seropositivity of PPR infection. There was no statistically significant difference in the seroprevalence of the disease between study areas and body condition score in this study. Conclusion: The present study finding revealed that a higher seroprevalence of PPR virus infection and this confirms PPR virus is widely prevalent in small ruminants and circulating in Afar region. Further studies should be carried out on the entire region to determine PPR seroprevalence and to develop appropriate control and eradication strategies of PPR disease.
Metal-Organic Framework-Based Oxygen Carriers with Antioxidant Activity Resulting from the Incorporation of Gold-Based Nanozymes
Authors: Liu Xiaoli , Domingues Nency P. , Oveisi Emad , Smit Berend , Hosta-Rigau Leticia 
Published: August 2022

Abstract

Blood transfusions are a life-saving procedure since they can preserve the body’s oxygen levels in patients suffering from acute trauma, undergoing surgery, receiving chemotherapy or affected by severe blood disorders. Due to the central role of haemoglobin (Hb) in oxygen transport, the so-called Hb-based oxygen carriers (HBOCs) are currently being developed for situations where donor blood is not available. In this context, an important challenge to be addressed is the oxidation of Hb into methaemoglobin (metHb) which is unable to bind and release oxygen. Since within red blood cells, this process is prevented by a set of protective enzymes such as superoxide dismutase and catalase, several research groups have considered incorporating these enzymes to create HBOCs with antioxidant properties. However, the use of biological enzymes has important limitations related to their high cost, potential immunogenicity or low stability in vivo. Thus, nanomaterials with enzyme-like properties (i.e., nanozymes (NZs)) have emerged as a promising alternative. In this work, we evaluate the antioxidant properties of gold (Au)-based NZs following incorporation within a type of HBOC previously reported by our group (i.e., Hb-loaded metal organic framework (MOF)-based nanocarriers (NCs)). We first prepare Au-loaded Au@MOF-NCs and demonstrate their ability to catalytically deplete two prominent reactive oxygen species (ROS) (i.e., hydrogen peroxide and superoxide radical) which exacerbate Hb’s autoxidation. Furthermore, these catalytic properties are retained over several cycles. Following loading with Hb, we show how the ROS-scavenging properties resulting from the Au-based NZs, translate into a decrease in metHb content. All in all, these results highlight the potential of NZs to create novel HBOCs with antioxidant protection which may find applications as a blood substitute in the future.
IsoTools – a ﬂexible workﬂow for long-read transcriptome sequencing analysis
Authors: Lienhard Matthias , Beucken Twan van den , Timmermann Bernd , Hochradel Myriam , Boerno Stefan , Caiment Florian , Vingron Martin , Herwig Ralf 
Published: August 2022

Abstract
Long-read transcriptome sequencing (LRTS) holds the promise to
reﬁne our understanding of alternative splicing, however, the nature of splicing is highly complex, and computational tools need to cope with this complexity by maximum ﬂexibility at diﬀerent steps of the work-ﬂow. For this purpose we developed IsoTools, a Python-based LRTS analysis package. IsoTools provides broad functionality for transcriptome reconstruction and quantiﬁcation of isoforms. Additionally, we implemented a graph-based method for the identiﬁcation of alternative
splicing events and a statistical approach based on the beta-binomial distribution for the detection of diﬀerential events. To demonstrate our methods, we generated PacBio LRTS data of human hepatocytes treated with the HDAC inhibitor valproic acid. Contrasted with short read RNA-seq, this analysis shows that LRTS provides valuable additional insights for a better understanding of alternative splicing, in particular with respect to complex novel and diﬀerential splicing patterns.
IsoTools is available at https://github.com/MatthiasLienhard/isotools.