bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disintegration of the Cav-1/β-catenin complex protects neuronal death by promoting β-catenin nuclear translocation after ischemia-reperfusion injury
Authors: Wang Wan , Guo Peng , Li Yihang , Li Ming , Li Pengpeng , Yan Tianqing , Wei Xuewen , Huang Linyan , qi suhua 
Published: September 2022

Abstract
The roles of Caveolin-1 (Cav-1) and Wnt/β-catenin signaling pathways in cerebral ischemia-reperfusion (I/R) injury are well established. Translocation of β-catenin into the nucleus is critical in regulating the apoptosis, repair, and nerve regeneration of neurons in the ischemic brain. Caveolin (Cav)-1 scaffold domain (residues 95-98) has been reported to interact with β-catenin (residues 330-337). However, the contribution of the Cav-1/β-catenin complex to I/R injury is currently unknown. To investigate the mechanism underlying the involvement of the Cav-1/β-catenin complex in the subcellular translocation of β-catenin and its subsequent effects on cerebral I/R injury, we treated ischemic brains with ASON (Cav-1 antisense oligodeoxynucleotides) or FTVT (a competitive peptide antagonist of the interaction Cav-1 and β-catenin). Our study showed that the binding of Cav-1 to β-catenin after I/R injury prevented the nuclear accumulation of β-catenin. The disruption of the Cav-1/β-catenin complex with ASON or FTVT after I/R injury significantly increased nuclear β-catenin. ASON and FTVT reduced the Ser33, Ser37 Thr41 phosphorylation of β-catenin, contributing to its proteasomal degradation; however, it increased the Tyr333 phosphorylation relating to its nuclear translocation. The above results indicated that the formation of the Cav-1/ β-catenin complex anchored the cytoplasmic β-catenin after I/R injury. Besides, both ASON and FTVT could attenuate neuronal death in ischemic brains. Our study suggests that targeting the interaction of Cav-1 with β-catenin could be a novel therapeutic strategy to protect against neuronal damage during cerebral injury.
KCNQ2 related-epilepsy: Report of nine cases and novel loss-of-function missense variants
Authors: Chokvithaya Suphalak , Caengprasath Natarin , Buasong Aayalida , Jantasuwan Supavadee , Santawong Kanokwan , Leela-adisorn Netchanok , Tongkobpetch Siraprapa , Ittiwut Chupong , Saengow Vitchayaporn , Kamolvisit Wuttichart , Boonsimma Ponghatai , Bongsebandhu-Phubhakdi Saknan , Shotelersuk Vorasuk 
Published: September 2022

Abstract
Mutations in KCNQ2 encoding for voltage-gated K channel subunits underlying the neuronal M-current, have been associated with infantile-onset epileptic disorders. The clinical spectrum ranges from self-limited neonatal seizures to epileptic encephalopathy and delayed development. Mutations of KCNQ2 could be either gain- or loss-of-function which require different therapeutic approaches. To better understand genotype-phenotype correlation, more reports of patients and their mutations with elucidated molecular mechanism are needed. Here, we report nine unrelated patients with KCNQ2-related epilepsy. Trio exome or genome sequencing successfully identified de novo heterozygous mutations in all patients. Two, p.N258K and p.G279D, had never been previously reported. The cellular localization study demonstrated that the surface membrane expression of Kv7.2 carrying either variant was decreased. Whole-cell patch-clamp analyses revealed that both variants significantly impaired Kv7.2 M-current amplitude and density, conductance depolarizing shift in voltage dependence of activation, membrane resistance, and membrane time constant (Tau), indicating a loss-of-function in both the homotetrameric and heterotetrameric with Kv7.3 channels. In addition, both variants also exerted dominant-negative effects in heterotetrameric with Kv7.3 channels. The two novel missense variants in KCNQ2 expand its mutational spectrum causing infantile-onset epileptic disorders and their functional consequences provide insights into their pathomechanism.
Mechanistic and Thermal Characterization of Acupuncture for Chemotherapy-Induced Peripheral Neuropathy as Measured by Quantitative Sensory Testing
Authors: Zhi W. Iris , Baser Raymond E. , Talukder Dristi , Mei Ying Zi (Jessy) , Harte Steven E. , Bao Ting 
Published: September 2022

Abstract
Purpose
Chemotherapy-induced peripheral neuropathy (CIPN) is a common and debilitating side effect of chemotherapy. Acupuncture is a promising nonpharmacological intervention for CIPN. However, the physiological effects of acupuncture treatment remain poorly understood. We examined the effects of acupuncture on CIPN using semi-objective quantitative sensory testing (QST).
Methods
We conducted a randomized controlled trial of real acupuncture (RA) and sham acupuncture (SA) compared to usual care (UC) in cancer survivors with moderate to severe CIPN. Treatment response was assessed with QST measures of tactile and vibration detection thresholds in hands and feet, thermal detection, and pain thresholds at weeks 0, 8, and 12. Constrained linear mixed model (cLMM) regression was used for statistical analysis.
Results
63 patients completed QST testing. At week 8, vibrational detection thresholds in feet were significantly lower in RA and SA (p = 0.019 and p = 0.046) than in UC, with no difference between RA and SA (p = 0.637). Both RA and SA also showed significantly higher cool thermal detection than UC (p = 0.008 and p = 0.013, respectively), with no difference between RA and SA (p = 0.790). No differences in tactile detection, vibrational detection in hands, warm thermal detection, and thermal pain thresholds were detected among the three arms at weeks 8 and 12.
Conclusion
QST demonstrated different patterns in RA, SA, and UC. After eight weeks of RA, we observed significant improvements in the vibrational detection threshold in feet and cool thermal detection threshold in hands compared to UC. No significant differences were seen when compared to SA.
Trial Registration:
ClinicalTrials.gov (NCT03183037); June 9, 2017
The definition, assessment and perception of patient trust by dental professionals– A scoping review
Authors: Yuan Siyang , John Deepti , Shambhunath Shambhunath , Humphris Gerry 
Published: September 2022

AbstractBackground: A trusting dentist-patient relationship is pivotal in providing person-centred care. This scoping review aims to identify how trust is defined, measured in the dental literature and how trust is perceived by dental professionals. Methods: Joanna Briggs Institute (JBI) framework was adopted. A search strategy was developed using MeSH terms and key words. Medline/PubMed, EMBASE, PsycINFO and CINAHL were searched. Data were synthesised using thematic analysis. Findings: Sixteen studies were included frequently using quantitative research methodology. Only 4 studies provided definition of trust. Many studies used either Dental Trust Scale or Dental Beliefs Survey to measure dentist-patient trust, although others developed their own items. Communication is a key aspect to measure trust. Limited research confirms that the dental professionals appreciated communication in building a trusting relationship with patients. Conclusion: No consensus was found on the definition of trust, nor on an assessment tool to measure dentist-patient trust. The limited evidence has found that dental professionals acknowledged the importance of effective communication in building a trusting alliance with patients. The scarcity of relevant research highlights the need for more robust investigations of trust in dental care.
Preclinical platform using a triple-negative breast cancer syngeneic murine model to evaluate immune checkpoint inhibitors
Authors: Bahadur Nar Bahadur , Park Nahee , Pandey Kamal , Bahadur Katuwal Nar , Kang Min Sil , Hong Sa Deok , Ghosh Mithun , Kim Seul-Gi , Cho Young Bin , Hur Jin , Kim Seung Ki , Moon Yong Wha 
Published: September 2022

Abstract
Purpose
To evaluate the feasibility of syngeneic mouse models of breast cancer by analyzing the efficacy of immune checkpoint inhibitors (ICIs) and potential predictive biomarkers.
Methods
Four syngeneic mouse models of triple-negative breast cancer (TNBC) JC, 4T1, EMT6 and E0771 cells were injected subcutaneously. When the tumor reached 50–100 mm3, each mouse model was divided into treatment (murine PD-1 antibody) and no-treatment control. Treatment group is further divided into the responder and nonresponder groups. Potential predictive biomarkers were evaluated by analyzing serum cytokines, peripheral blood T cells and tumor infiltrating immune cells.
Results
The JC model showed the highest tumor response rate (40%, 4/10) of syngeneic models: 4T1 (36%, 4/11), EMT6 (36%, 4/11), or E0771 model (23%, 3/13). Early change of tumor size at 7 days post PD-1 inhibitor treatment predicted the final efficacy of PD-1 inhibitor. Peripheral blood CD8 + and CD4 + T cells with or without Ki67 expression at 7 days post-PD-1 inhibitor treatment were higher in the finally designated responder group than in the nonresponder group. At the time of sacrifice, analyses of tumor infiltrating lymphocytes consistently supported these results. Furthermore, serum IFN-γ at 7 days post-PD-1 inhibitor treatment was also higher in responders than in nonresponders, suggesting that early changes of these markers could be predictive biomarkers of the final efficacy of ICIs.
Conclusions
Our syngeneic mouse model of TNBC is a feasible preclinical platform to evaluate ICI efficacies combined with other drugs and predictive biomarkers in the screening period of immune-oncology drug development.
Influence of land-sea breeze on long-term PM2.5 prediction in central and southern Taiwan using composite neural network
Authors: Kibirige George William , Huang Chiao-Cheng , Liu Chao-Lin , Chen Meng-Chang 
Published: September 2022

Abstract
PM2.5 prediction plays an important role for governments in establishing policies to control the emission of excessive atmospheric pollutants to protect the health of citizens. However, traditional machine learning methods that use data collected from ground-level monitoring stations have reached their limit with poor model generalization and insufficient data. We propose a composite neural network trained with aerosol optical depth (AOD) and weather data collected from satellites, as well as interpolated ocean wind features. We investigate the model outputs of different components of the composite neural network, concluding that the proposed composite neural network architecture yields significant improvements in overall performance compared to each component and the ensemble model benchmarks. The monthly analysis also demonstrates the overall superiority of the proposed architecture for the southern and central Taiwan stations in the months when land-sea breeze events frequently occur.
Aspartate reduces liver inflammation and fibrosis by suppressing the NLRP3 inflammasome pathway via upregulating NS3TP1 expression
Authors: Zhou Li , Zhao Jing , Han Ming , Ma Anlin , yang song , Zeng yilan , Cheng Jun 
Published: September 2022

Abstract
Aspartate (Asp) can act on liver Kupffer cells, inhibit NOD-like receptor-P 3 (NLRP3) inflammatory bodies, and improve liver inflammation in acute hepatitis. However, the effect of Asp on the role of hepatic stellate cells (HSCs) in the pathogenesis of liver fibrosis in chronic liver injury remains unexplored. This study aimed to investigate the effects of Asp on CCl4‑induced liver fibrosis in mice and HSCs via the NF-κB/ NLRP3 signaling pathway. Liver fibrosis was induced in C57BL/6J mice by intraperitoneally (IP) injecting 0.5 mL/kg 2% CCl4 three times weekly for 8 weeks. Asp was administered to mice by gavage once every morning for 4 weeks. Masson's trichrome staining, Sirius red staining and hematoxylin and eosin staining were used to detect and analyze the pathological changes in liver tissues. Western blot analysis and immunohistochemistry were applied to determine the protein expression levels of α‑smooth muscle actin (α‑SMA), collagen Ⅲ (COL Ⅲ), NLRP3, and IL-1β. Also, reverse transcription‑quantitative PCR was performed to detect the mRNA expression levels. In the liver fibrosis model, the pathological changes in liver tissues improved following treatment with Asp. A marked decrease was observed in protein and mRNA expression levels of α‑SMA, COL Ⅲ, NLRP3, and IL-1β. In addition, HSCs were treated with Asp. The expression levels of α‑SMA, COL Ⅲ, NLRP3, and IL-1β reduced in dose‑ and time-dependent manners. Furthermore, Asp upregulated the expression of NS3TP1 in vivo and in vitro, and NS3TP1 had a significant inhibitory effect on liver fibrosis. Asp attenuated liver fibrosis and reduced collagen production by suppressing the NF-κB/ NLRP3 signaling pathway via upregulating the expression of NS3TP1.
Comparison of Real-time Feedback and Debriefing by Video Recording on Basic Life Support Skill in Nursing Students
Authors: Ghaderi Mohammad Sajjad , Malekzadeh Javad , Mazloum Seyedreza , pourghaznein Tayebe 
Published: September 2022

Abstract
Background
Cardiopulmonary resuscitation skills have a direct impact on its success rate. Choosing the right method to acquire this skill can lead to effective performance. This investigation was conducted to compare the effect of Real-time feedback and Debriefing by Video Recording on basic life support skills in nursing students.
Methods
This quasi-experimental study was performed on 67 first year nursing students. First, a theoretical BLS training session was held for the all participants, at the end of session the pre-test was taken. Students were randomly assigned to two groups. A 4-hour practical basic resuscitation training session was conducted in the Real - time feedback group as well as the Debriefing by video recording group, and at the end of the training, a post-test was taken from each group. Each group received a post-test. Data were analyzed using SPSS 25 software.
Results
Results showed a significant difference between mean (SD) of feedback in pre-test and post-test (p < 0.001) and in the feedback group there was a significant difference between mean (SD) in pre-test and post-test (p < 0.001), respectively. In addition, there was no significant difference between the mean score of basic life support skill in Real-time feedback and Debriefing by Video Recording.
Conclusions
Both Real-time feedback and Debriefing by Video Recording were effective on basic life support skill.
Safety injections of Nuclear Medicine radiotracers: towards a new modality for a real-time detection of extravasation events and 18F-FDG SUV data correction.
Authors: Iori Mauro , Grassi Elisa , Lorenzo Piergallini , Meglioli Greta , Botti Andrea , Sceni Giada , Cucurachi Noemi , Verzellesi Laura , Finocchiaro Domenico , Versari Annibale , Fraboni Beatrice , Fioroni Federica 
Published: September 2022

Abstract

Background18F-FDG PET/CT imaging allows to study oncological patients and their relative diagnosis through the Standardized Uptake Value (SUV) evaluation. During radiopharmaceutical injection, an extravasation event may occur, making the SUV value less accurate and possibly leading to severe tissue damage. The aim of the study was to propose a new technique to monitor and manage these events, in order to evaluate a correction to the calculated SUV value. Methods A cohort of 70 patients undergoing18F- FDG PET/CT exams was enrolled. Two portable detectors were secured on the patients' arms. The dose-rate (DR) time curves on the injected DRinand contralateral DRconarm were acquired during the first 10 minutes of injection. Such data were processed to calculate the parameters ΔpinNOR= (DRinmax- DRinmean)/ DRinmaxand ΔRt= (DRin(t) - DRcon(t)), where DRinmaxis the maximum DR value, DRinmeanis the average DR value in the injected arm. OLINDA software allowed dosimetric estimation of the dose in the extravasation region. The estimated residual activity in the extravasation site allowed the evaluation of the SUV's correction value and to define an SUV correction coefficient. Results Four cases of extravasations were identified for which ΔRt[(390 ± 26) µSv/h], while ΔRt[(150 ± 22) µSv/h] for abnormal and ΔRt[(24 ± 11) µSv/h] for normal cases. The ΔpinNORshowed an average value of 0.44 for extravasation cases and an average value of (0.91 ± 0.06) and (0.77 ± 0.23) in normal and abnormal classes, respectively. The percentage of SUV reduction (SUV%CR) ranges between 0.3% and 6%. The calculated self-tissue dose values range from 0.027 Gy to 0.573 Gy, according to the segmentation modality. A similar correlation between the inverse of ΔpinNORand the normalised ΔRtwith the SUV correction coefficient was found. Conclusions The proposed metrics allowed to characterised the extravasation events in the first few minutes after the injection, providing an SUV correction when necessary. We also assume that the characterization of the DR-time curve of the injection arm is sufficient for the detection of extravasation events. Further validation of these hypotheses and key metrics is recommended in larger cohorts.
A Fourier series Non-Stationary Coherency Model with respect to site conditions for the horizontal component of ground motion
Authors: Lin Qixiang , Peng Gang , Zhang Qi , Chen Denghong 
Published: September 2022

Abstract
A Fourier series non-stationary coherency model with respect to the site conditions for the horizontal component was developed. The evolution of the model was achieved using parameters related to the site, inter-station distance, and time. First, based on the simulation of a non-stationary ground-motion field considering the wave-passage effect and site-response effect, the approach used for the estimation of coherency using wavelet transform is presented. Subsequently, the Fourier series non-stationary coherency model is formulated. The parameters are considered piecewise constant variables to represent the non-stationarity of the estimated coherency. The entire non-stationary strong ground motion was divided into ten parts according to the cumulative energy. The effects of the site and inter-station distance on the proposed model are presented. Parameters related to the site and inter-station distance were obtained. Finally, the proposed model is compared with the ground motion records at the SMART-1 array during Event 45, the stationary model, and the data from the Argostoli rock site dense array. This indicates that the Fourier series non-stationary coherency model with respect to site conditions for the horizontal component can well match the correlation of the realistic spatially variable seismic ground motion, which is related to the site, time, and inter-station distance.
Application of the Child Community Health Inclusion Index for measuring health inclusion of children with disabilities in the community: A feasibility study
Authors: Yoo Paul Yejong , Majnemer Annette , Wilton Robert , Ahmed Sara , Shikako Keiko 
Published: September 2022

Abstract
Background
Participation in the community is a fundamental human right for children with disabilities and is a key component of their health and development. Inclusive communities can enable children with disabilities to participate fully and effectively. The Child Community Health Inclusion Index (CHILD-CHII) is a comprehensive assessment tool developed to examine the extent to which community environments foster healthy, active living for children with disabilities.
Objectives
To assess the feasibility of applying the CHILD-CHII measurement tool across different community settings.
Methods
Participants recruited through maximal representation, and purposeful sampling from four community sectors (Health, Education, Public Spaces, Community Organizations) applied the tool on their affiliated community facility. Feasibility was examined by assessing length, difficulty, clarity, and value for measuring inclusion; each rated on a 5-point Likert scale. Participants provided comments for each indicator through the questionnaire and a follow-up interview.
Results
Of the 12 participants, 92% indicated that the tool was ‘long’ or ‘much too long’; 66% indicated that the tool was clear; 58% indicated that the tool was ‘valuable’ or ‘very valuable’. No clear consensus was obtained for the level of difficulty. Participants provided comments for each indicator.
Conclusion
Although the length of the tool was regarded as long, it was seen to be comprehensive and valuable for stakeholders in addressing the inclusion of children with disabilities in the community. The perceived value and the evaluators’ knowledge, familiarity, and access to information can facilitate use of the CHILD-CHII. Further refinement and psychometric testing will be conducted.
Characteristics and outcomes of patients who did not respond to a national spine surgery registry.
Authors: Kaur Simran , Alhaug Ole Kristian , Dolatowski Filip , Solberg Tore , Lønne Greger 
Published: September 2022

Abstract
Background
Loss to follow-up may bias outcome assessments in medical registries. This cohort study aimed to analyze and compare patients who failed to respond with those that responded to the Norwegian spine surgery register (NORspine).
Methods
We analyzed a cohort of 474 consecutive patients operated for lumbar spinal stenosis at four public hospitals in Norway during a two-year period. These patients reported sociodemographic data, preoperative symptoms, and Oswestry Disability Index (ODI), numerical rating scales (NRS) for back and leg pain to NORspine at baseline and at 3 and 12 months postoperatively. At a median of 50 (36–64) months after surgery, we contacted all patients who did not respond to NORspine after 12 months. Those who responded were termed responsive non-respondents and compared to 12 months respondents.
Results
123 (27%) of patients did not respond to NORspine 12 months after surgery, and 64 (52%) of those responded to our renewed survey at a median of 50 (36–64) months after surgery. At baseline, non-respondents were younger 63 (SD 11.7) vs 68 (SD 9.9) years, mean difference (95% CI) 4.7 years (2.6 to 6.7); p = < 0.001. Non-respondents were more frequently smokers 41 (30%) vs 70 (21%) RR (95%CI) = 1.40 (1.01 to 1.95); p = 0.044. There were no other relevant differences in other sociodemographic variables or preoperative symptoms. We found no differences in effect of surgery on non-respondents vs respondents (ODI (SD) = 28.2 (19.9) vs 25.2 (18.9), MD (95%CI) = 3.0 ( -2.1 to 8.1); p = 0.250.
Conclusion
We found that 27% of patients did not respond to NORspine 12 months after surgery for lumbar spinal stenosis. Non-respondents were somewhat younger and more frequently smokers. However, responsive non-respondents reported similar clinical outcomes compared to those who responded.