Pengaruh Pemberian Virgin Coconut Oil (VCO) Terhadap Kadar Hemoglobin dan Jumlah Eritrosit Tikus Putih Jantan Galur Wistar Yang Dipapar Asap Rokok
Authors: Simorangkir Saharnauli Janna Verawaty 
Published: June 2022

Abstrak

Pendahuluan: Virgin Coconut Oil (VCO) merupakan minyak kelapa murni yang berasal dari olahan daging kelapa segar. VCO memiliki kandungan antioksidan yang tinggi yang dapat berperan sebagai radical scavengers atau peredam reactive oxygen spesies (ROS). ROS terbesar adalah asap rokok yang dapat menyebabkan stress oksidatif bagi eritrosit. Tujuan penelitian: untuk mengetahui bagaimana pemberian VCO berpengaruh sebagai antioksidan terhadap kadar hemoglobin dan jumlah eritrosit tikus putih jantan galur wistar yang dipapar asap rokok. Metode: Desain penelitian ini adalah posttest only control group design. Sampel dalam penelitian sebanyak 24 ekor tikus jantan galur wistar usia 2-3 bulan yang dibagi menjadi 4 kelompok yang diberi perlakuan berbeda selama 14 hari yaitu kelompok kontrol negatif diberi pakan standart, kelompok kontrol positif diberi pakan standart dan dipapar asap rokok, kelompok perlakuan 1 (P1) dipapar asap rokok dan diberi VCO sebanyak 0,45 ml, kelompok P2 dipapar asap rokok dan diberi VCO sebanyak 0,9 ml. Pada hari ke-15 tikus dibedah untuk diperiksa darahnya. Kemudian data dianalisis dengan uji Anova pada tingkat kepercayaan 95%. Hasil: Secara statistik tidak terdapat perbedaan yang signifikan rerata kadar hemoglobin dan jumlah eritrosit tikus putih jantan galur wistar. Namun berdasarkan hasil pemeriksaan darah, kadar hemoglobin dan jumlah eritrosit kelompok control positif lebih rendah dibandingkan 3 kelompok lainnya. Serta kadar hemoglobin dan jumlah eritrosit yang diberi VCO dengan dosis maksimal 0,9 ml lebih tinggi dibandingkan dengan menggunakan dosis 0,45 ml. Kesimpulan: Pemberian VCO tidak dapat mencegah penurunan kadar hemoglobin dan jumlah eritrosit tikus putih jantan galur wistar yang dipapar asap rokok.
Abstract

Introduction: Virgin Coconut Oil(VCO) is virgin coconut oil which is derived from processed fresh coconut meat. VCO has a high antioxidant content where these antioxidants act as radical scavengers or reducer of reactive oxygen species (ROS). The biggest ROS is cigarette smoke which can causing oxidative stress for erythrocytes. Aims : to determine how the administration of VCO has an effect as an antioxidant on hemoglobin levels and the number of erythrocytes of male white rats of the Wistar strain exposed to cigarette smoke.Method: The design of this study was a posttest only control group design. The sample in this study were 24 male wistar rats aged 2-3 months. This study was divided into 4 groups that were given different treatments for 14 days, namely the negative control group was given standard feed, the positive control group was given standard feed and was exposed to cigarette smoke, treatment group 1 (P1) was exposed to cigarette smoke and given virgin coconut oil (VCO). 0.45 ml, group P2 was exposed to cigarette smoke and given 0.9 ml of VCO. On the 15th day the rats were dissected to check their blood. Then the data were analyzed by Anova test at 95% confidence level. Results: Statistically, there was no significant difference in the mean hemoglobin level and erythrocyte count of Wistar male white rats. However, based on the results of blood tests, the hemoglobin and erythrocyte counts in the positive group were lower than the other 3 groups. As well as hemoglobin levels and the number of erythrocytes given VCO with a maximum dose of 0.9 ml higher than using a dose of 0.45 ml. Conclusion: Giving VCO could not prevent a decrease in hemoglobin levels and the number of erythrocytes of male Wistar strain rats exposed to cigarette smoke.