bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project
Authors: Obadia Thomas , GUTIERREZ-BUGALLO Gladys , Duong Veasna , NUNEZ Ana , FERNANDES Rosilainy , Kamgang Basile , Héry Lyza , GOMARD Yann , ABBO Sandra , JIOLLE Davy , GLAVINIC Uros , DUPONT-ROUZEYROL Myrielle , Atyame Célestine , POCQUET Nicolas , BOYER Sébastien , DAUGA Catherine , VAZEILLE Marie , YEBAKIMA André , White Michael , Koenraadt Constantianus , MAVINGUI Patrick , Vega-Rúa Anubis , VERONESI Eva , Pijlman Gorben , PAUPY Christophe , BUSQUETS Nuria , Lourenço-de-Oliveira Ricardo , de Lamballerie Xavier , FAILLOUX Anna-Bella 
Published: May 2022

Abstract
First identified in 1947, Zika virus took roughly 70 years to cause a pandemic unusually associated with virus-induced brain damage in newborns. Zika virus is transmitted by mosquitoes, mainly Aedes aegypti, and secondarily, Aedes albopictus, both colonizing a large strip encompassing tropical and temperate regions. As part of the international project ZIKAlliance initiated in 2016, 50 mosquito populations from six species collected in 12 countries were experimentally infected with different Zika viruses. Here, we show that Ae. aegypti is mainly responsible for Zika virus transmission having the highest susceptibility to viral infections. Other species play a secondary role in transmission while Culex mosquitoes are largely non-susceptible. Zika strain is expected to significantly modulate transmission efficiency with African strains being more likely to cause an outbreak. As the distribution of Ae. aegypti will doubtless expand with climate change and without new marketed vaccines, all the ingredients are in place to relive a new pandemic of Zika.
Utilization of Fmoc-3f-phe Hydrogel for Encapsulation of Zanthoxylum Armatum and Cinnamomum Camphora Oil for Enhancing Their Antibacterial Activity
Authors: Shakya Nasla , B.C. Santosh , Joshi Susan , Rajbhandary Annada 
Published: April 2022

Abstract
ObjectiveWhile essential oils have many applications in medicine, not many studies have been done in the past to address issues of active targeting, enhancing bioavailability and reducing toxicity at higher concentrations. Herein, we used Fmoc-3F-Phe amino acid hydrogels to address such issues by encapsulating essential oils, Zanthoxylum armatum and Cinnamomum camphora, in its system and allowing sustained-release of these oils onto bacterial assays of E. coli ATCC 25922, P. hauseri NBRC 3851, M. luteus KACC 13377, and B. subtilis ATCC 66333 for probing enhanced antibacterial properties of the oils by prolonging its efficacy through controlled-release mechanism. ResultsWe found that while Zanthoxylum oil showed no particular difference in enhancing the antibacterial property against the three fast growing bacteria, however profound variation was observed against slow growing bacteria B. subtilis where the hydrogel encapsulated oil was able to retain the antibacterial property of the oil for longer time while incomparison the oil applied directly could not. Even for highly volatile camphor oil, the oil itself failed to show any antibacterial property with direct use, however the hydrogel encapsulated oil was able to show excellent antibacterial property for B. subtilis and M. luteus through prohibition of sublimation via encapsulation.
Exploring misleading online oral health information: a content analysis
Authors: Lotto Matheus , Jorge Olivia Santana , Machado Maria Aparecida Andrade Moreira , Cruvinel Thiago 
Published: April 2022

Abstract
Objective: This study aimed to identify and characterize misleading online oral health information. Materials and Methods: 410 websites published in English were retrieved by Google Advanced Search and screened by 2 independent investigators, who compiled false messages through content analysis. Afterward, 318 content items were divided in consensus as G1 - informationally-oriented misinformation, G2 - misinformation with interests, G3 - misinformation with interests produced or propagated by dental professionals, and G4 – misinformation with political interests. Their presence on social media and fact-checking agencies was determined using CrowdTangle and Snopes. Data were evaluated by descriptive analysis and Cramer’s V test to compare distinct categories. Results: The main issues associated with misleading content items were gum diseases (12.0%), root canal treatment (11.6%), toothache (10.4%), fluoride (10.4%), and dental caries (9.8%), highlighting recommendations for natural products usage, toxicity concerns, and anti-fluoridation propaganda. Most misinformation (41.9%) was allocated in G3, which exhibited a statistically higher frequency of financial interests than other groups. Besides, falsehoods were considerably identified on Facebook (62.9%) and Instagram (49.4%), especially G3 and G4. Nevertheless, Snopes has already debunked only 5.9% of these content items.Conclusions: Misleading information was predominantly produced or propagated by dental professionals mainly motivated by financial interests and usually linked to alternative/natural treatments diffusion. Although these items were shared on social media, fact-checking agencies seemed to have insufficient knowledge about their dissemination.Clinical Relevance: Dental teams need to be conscious of false oral health content to improve the quality of their relationship with patients through adequate communication.
Lactate from glucose metabolism regulates osteogenic differentiation of human periodontal ligament stem cells
Authors: Deng Xin , Kato Hirohito , Taguchi Yoichiro , Nakata Takaya , Umeda Makoto 
Published: May 2022

Abstract
Objectives: Periodontal ligament stem cells (PDLSCs), as one of mesenchymal stem cells in the oral cavity, are closely associated with periodontal hard tissue regeneration. However, the effect of local tissue glucose deficiency on periodontal tissue regeneration, such as immediately after surgical operation, is still unknown. Therefore, this study sought to investigate the effect of a low-glucose environment on the proliferation and osteogenic differentiation of PDLSCs.Material and methods: This study used medium at five glucose concentrations (100, 75, 50, 25 and 0 mg/dL) and focused on the effects on PDLSCs of changes in lactate in the low-glucose environment. Lactate uptake by PDLSCs in a standard glucose environment was suppressed by the addition of AZD3965, a monocarboxylate transporter-1 inhibitor. Results: We investigated cell proliferation, migration and assessed osteogenic differentiation factors. Furthermore, PDLSC lactate production was suppressed under low-glucose conditions. We compared that condition to the low-glucose environment to assess lactate's effect on the proliferation and osteogenic differentiation of PDLSCs. The low-glucose environment decreased PDLSC lactate production and inhibited PDLSC osteogenic differentiation. In low glucose environment, the phosphorylation of extracellular signal-regulated kinase, protein kinase B, and the activation of hypoxia-inducible factor 1α signaling pathways were inhibited, also, AZD3965 inhibited the activation of these signaling pathways. Conclusion: The results of this study suggested that lactate production by glucose metabolism is involved in periodontal tissue regeneration.Clinical relevance: The findings of close relationship about lactate metabolism in periodontal tissue may contribute new insights into future periodontal regenerative therapies. Lactate may be a new treatment option for periodontal regenerative therapy in the future.
hsa_circRNA_102049 promotes cell proliferation, migration and induces EMT via PI3K-AKT signaling pathway in osteosarcoma
Authors: Chen Jianquan , Chen Rongbin , He Mincong , Lin Xinyuan , Lv Zhouming , Wu Yongsheng , Chen Lin 
Published: May 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Unilateral internal carotid artery dissection with syncope as the first symptom
Authors: Xia Junyang , Jiang Xin , Pan Haojun , Liu Peipei 
Published: April 2022

Abstract
Internal carotid artery dissection(ICAD) is a series of pathological processes caused by intimal tear of the internal carotid artery. Syncope refers to a transient loss of consciousness caused by transient global brain hypoperfusion. ICAD with syncope is rare. Here we present a case of unilateral ICAD with syncope as the first symptom. We also review the literature and discuss the pathogenesis of syncope in ICAD patients. The main message we want to convey to readers is that unilateral ICAD can have syncope as the first symptom, which should not be ignored in the differential diagnosis of syncope. Furthermore, the pathogenesis of syncope in ICAD is more likely to be that subintimal blood flow can easily increase the mechanical stretch of adventitia, stimulate the baroreceptors and cause hyperactive carotid sinus reflex.
A Somatic Mutation-Derived LncRNA Signatures of Genomic Instability Predicts the Prognosis and Tumor Microenvironment Immune Characters in Hepatocellular Carcinoma
Authors: Jin Chuan , Zhao Jian-Sen , Huang Xu-Qi , Yang Xian-Zi , Niu Fei-Yu , Lin Jin-Rong , Ma Lei , Shi Yan-Xia , Li Xiao-Shan , Jiang Peng , Gao Sha , Li Feng , Song Ye 
Published: May 2022

Abstract
Background:Hepatocellular carcinoma (HCC) is an aggressive carcinoma with genome instability. Long non-coding RNAs (LncRNAs) have been functionally associated with genomic instability in cancers. However, the identification and prognostic value of lncRNAs related to genome instability have not been explored in hepatocellular carcinoma. In this study, we aim to identify a genomic instability-related lncRNA signature for predicting prognosis and the efficacy of immunotherapy in HCC patients.Methods:According to the somatic mutation and transcript data of 364 patients with HCC, we determined differentially expressed genome instability-related lncRNAs (GInLncRNAs). Gene Ontology (GO) enrichment analyses and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed the potential functions of genes co-expressed with those lncRNAs involved in cancer development and immune function. We further determined a genome instability-related lncRNA signature (GInLncSig) through Cox regression analysis and LASSO regression analysis. Thereafter, we performed correlation analyses with mutations, clinical stratification analyses, survival analysis to evaluate GInLncSig predictive function. Subsequently, we construct a nomogram model for prognostic assessments of patients with HCC. Finally, we performed Immunocytes infiltration analysis, gene set enrichment analysis (ssGSEA) of immunity circle associated pathways, and T cell-inflamed score to explore GInLncSig’s potential value of guiding immunotherapy.Results:We identified 11 independent prognosis-associated GInLncRNAs (AC002511.2, LINC00501, LINC02055, LINC02714, LINC01508, LOC105371967, RP11_96A15.1, RP11_305F18.1, RP11_342M1.3, RP11_432J24.3, U95743.1) to construct a GInLncSig. According to the risk score calculated by GInLncSig, the high-risk group was characterized by a higher somatic mutation count, significantly poorer clinical prognosis, higher T cell-inflamed score, and specific tumor immune infiltration status compared with the low-risk group. Furthermore, we constructed a nomogram model to improve the reliability and clinical utility for predicting the prognosis of patients with HCC. Conclusion：our study established a reliable prognostic prediction signature that could be a tool for prognosis prediction and a promising predictive biomarker of immunotherapy in hepatocellular carcinoma.
Compressibilities along the magnetite-magnesioferrite solid solution
Authors: Melai Caterina , Boffa-Ballaran Tiziana , Uenver-Thiele Laura , Kurnosov Alexander , Chumakov Aleksandr I. , Bessas Dimitrios , Frost Daniel J. 
Published: May 2022

Abstract
In order to calculate the thermodynamic properties for recently discovered high-pressure mixed valence iron-oxides in the system Fe-Mg-O, information on the equation of state of precursor inverse spinel phases along the magnetite-magnesioferrite join is needed. The existing equation of state data, particularly for magnesioferrite, are in poor agreement and no data exist for intermediate compositions. In this study, the compressibility of nearly pure magnesioferrite as well as of an intermediate \({{\text{M}\text{g}}_{0.5}\text{F}\text{e}}_{0.5}^{2+}{\text{F}\text{e}}_{2}^{3+}{O}_{4}\) sample have been investigated for the first time up to approximately 13 and 19 GPa respectively, using single crystal X-ray diffraction in a diamond anvil cell. Samples were produced in high-pressure experiments to promote a high level of cation ordering, with resulting inversion parameters of x = 0.892(2) and x = 0.98(3) for the endmember and intermediate composition, respectively. The room pressure unit cell volumes, V0, and bulk moduli, KT0, could be adequately constrained using a 2nd order Brich-Murnaghan equation of state, which yield V0 =588.97 (8) Å3 and KT0 = 178.4(5) GPa for magnesioferrite and V0 = 590.21(5) Å3 and KT0 = 188.0(6) GPa for the intermediate composition. As magnetite has KT0 = 180(1) GPa (Gatta et al., 2007), this means the variation in KT0 across the magnetite-magnesioferrite solid solution is significantly non-linear, in contrast to several other Fe-Mg spinels. The larger incompressibility of the intermediate composition compared to the two endmembers may be a peculiarity of the magnetite-magnesioferrite solid solution caused by an interruption of Fe2+-Fe3+ electron hopping by Mg cations substituting in the octahedral site.
Aerosol boxes for Airway management in coronavirus disease patients: a clinical retrospective study in Mexico
Authors: Duarte-Medrano Gilberto , Sorbello Massimiliano , Susunaga-Hope Rafael Renato , Fuentes-Castro Paula Ivette , Avila-OrtIZ Beatriz C , Velasco-Godinez Aida Paola , Contreras-Garcia Wendy Y , Pineda-Castillo Miguel Alejandro , Urdaneta Felipe , Téllez-Ávila Félix Ignacio 
Published: April 2022

Abstract
Introduction: Significant concerns raise for the healthcare workers involved in airway management of patients diagnosed with coronavirus 2019 disease (COVID-19).Due to shortages of personal protective equipment (PPE), barrier enclosure systems such as aerosol box (AB) have been proposed worldwide. Aim of this study was to evaluate our experience using AB as protective equipment in patients with COVID-19 in a third level center in Mexico.Methods: Retrospective study of COVID-19 patients requiring airway management using an AB in the Hospital Central Sur de Alta Especialidad de Pemex in Mexico City from March 1 to June 1, 2020. Antropometric data, pre-intubation vital signs and laboratory tests, intubation success rate, complications associated with AB and patients’ mortality were recorded. A survey was administered after airway management for subjective evaluation of AB.Results: 39 patients for a total of 40 intubations were documented. 31 (77.5%) were men, with mean age of 61.65 years; successful intubation occurred in 39 (97.55%) of the procedures, AB was used in 36 (90%) of intubations, with success in 28 (70.0%); A Cormack-Lehane grade 3 view was recorded in 18 patients (46.2%), and during the procedure the AB had to be removed in 8 (22.2%) cases, with migration documented in 91.6% of cases. The 30-day mortality was 48.71%, with 23.0% of patients discharged. 83.3% of surveyed anesthesiologists reported significant limitations in manipulating airway devices with AB used.Conclusion: Our data indicate that in clinical practice use of AB may hinder airway management, decreases the intubation success rate and may also result in patients’ injury. Further studies are necessary to validate use of AB in clinical practice, and they should not replace certified PPE.
Study on the causes of death of hospitalized neonates from 2012 to 2021 at a tertiary hospital in east China
Authors: Wang Huawei , Wu Zhixin , Geng Haifeng , Zhu Xiaoli , Zhu Xueping 
Published: April 2022

Abstract
Background: This study aimed to analyze the clinical data of neonatal deaths in a tertiary children’s hospital in east China by investigating the causes and differences among neonatal deaths over the past ten years. By estimating the levels and trends of neonatal deaths, the treatment level could be improved, and preventive measures could be taken to decrease the mortality rates of hospitalized neonates.Methods: The clinical data of deceased neonates admitted to the Department of Neonatology, Children’s Hospital of Soochow University, were collected between January 2012 and December 2021. We estimated and analyzed the number of neonates dying during the first 28 days of life as a function of their gestational age, birth weight, and age at death.Results: From 42,004 hospitalized neonates, we identified 469 cases of death (1.12%). The mortality rate of hospitalized neonates decreased from 2.15% in 2012 to 0.66% in 2021. Similarly, the mortality rate of term neonates decreased from 1.26% to 0.53%, and that of preterm neonates was reduced from 6.03% to 2.67%. The proportion of neonates with gestational age <32 weeks gradually increased from 2012 to 2021, but for neonates with gestational ages ≥37 weeks, they were lower between 2017 and 2021 than between 2012 and 2016 (P<0.05). The proportion of dead neonates with birth weights less than 1,499g gradually increased from 2012 to 2021 but was greater between 2017 and 2021 than between 2012 and 2016. While neonatal deaths within 1 day progressively increased, the opposite was true for deaths between 8 and 28 days. The incidence of pregnancy complications, gestational diabetes, and the using of corticosteroids before birth were higher between 2017 and 2021 than from 2012 to 2016 (P < 0.05). The proportion of preterm with neonatal respiratory distress syndrome (NRDS) increased, although neonatal asphyxia was rarer in the second than the first half of the investigated decade (P<0.05).In the first half, the leading causes of neonatal death in declining frequency were infectious diseases, neonatal asphyxia, preterm/NRDS, severe congenital malformations, and neonatal necrotizing enterocolitis. In contrast, from 2017 to 2021, the leading causes of neonatal death have been preterm/NRDS, infectious diseases, neonatal asphyxia, neonatal necrotizing enterocolitis, and severe congenital malformations.Conclusions: With the advancement of neonatal medicine, the hospitalized neonatal mortality rate has significantly decreased in the last 10 years. Infectious diseases and neonatal asphyxia follow preterm/NRDS as the most frequent causes of neonatal deaths in recent years. Therefore, we should strengthen the prevention and control of infection, encourage cooperation with obstetrics departments to avoid premature deliveries, and improve resuscitation.
Design and preparation of micro-nanostructures on the surface of titanium alloy artificial bone and analysis of the frictional properties of the processed surface
Authors: Chen Rifan , Liu Zhiqiang , Zhao Fuhai , Wu Youchao , Zhang Jiajun 
Published: May 2022

Abstract
In this paper, we use laser processing method to process ortho-hexagonal microstructure on the surface of titanium alloy, and determine the optimal processing parameters according to the integrity and clarity of ortho-hexagonal processing. According to the experimental analysis, it is obtained that the processing speed is 300 mm/s, the power is 20 W and the frequency is 30 KHZ, the processing effect is the best. The optimized processing parameters were used to study the relationship between the weave height and the number of processing times. Using the method of multiple acid etching to prepare nanopores on the surface of titanium alloy, it is found that multiple acid etching can not only prepare nanoscale holes on the surface of titanium alloy, but also can well solve the slag left on the surface of titanium alloy by laser ablation.
On top of that, the effect of friction reduction of micro-nano structures on the surface of titanium alloy with different shapes, edge lengths and heights is also investigated. Both theoretically and experimentally, the microshaped surfaces are analyzed to have better wear resistance and lower average friction coefficient compared with smooth surfaces. The effect of single factors on the average friction coefficient was analyzed, and the results showed that the average friction coefficient decreases and then increases with the increase of side length and height of the weave, and the average friction coefficient of the ortho-hexagonal array weave is the smallest and the average friction coefficient of the circular array weave is the largest among the four shapes. The effect of the interaction of factors on the average friction coefficient was investigated, and the results showed that the optimal average friction coefficient was 0.0902, corresponding to the characteristic values of 0.66 for the shape, 200 μm for the height, and 60 μm for the height.
Salidroside and Compound C combination for treatment of cerebral ischemia-reperfusion injury by remodeling gut microbiota to regulate the brain–gut barriers
Authors: li huanhuan , Deng Hongxian , Fang Wenlan , Huang Xiting , Zhao Huanying , Liu Ruiying , Tong Yan 
Published: April 2022

Abstract
Background: It was reported that Salidroside and AMPK inhibitor Compound C alone have therapeutic effects on ischemic stroke. This paper explores the effect of salidroside combined with Compound C on cerebral ischemia-reperfusion injury in rats and based on the intestinal microecology its mechanism of action. Methods: Male Sprague-Dawley rats were randomly divided into 5 groups: ①sham-operated group; ②model group; ③ Salidroside group; ④Inhibitor group; ⑤Salidroside + inhibitor group. The rats in each group were given prophylactic intraperitoneal injection or normal saline for 7 days, and then the MCAO model was established. After the model was successfully established, each group continued to be treated with the corresponding solution for 7 days. After the last administration, the neurological function of the rats was detected, and then the whole brain was taken for sectioning, and the cerebral infarction area was measured after triphenyltetrazolium chloride staining; the brain tissue and colon tissue were taken for section analysis and Hematoxylin-Eosin staining; serum D-LA FABP, LPS, LBP concentration , DAO activity , IFN-γ, TNF-α, IL-6 and IL-10 concentration were detected by Elisa; claudin-5 mRNA, occludin mRNA and zo-1 mRNA expression levels were detected by Real-time PCR; gut microbiota analysis by 16S rRNA amplicon sequencing. Results: SAL and CC combination improved neurological function and relieved cerebral infarction; SAL and CC combination affected the α and β diversity of gut microbiota, increased beneficial bacteria, reshaped the gut microbiome and improved gut microbial dysbiosis; SAL and CC combination improved the histopathology of rat brain and colon, prevented the destruction of the brain-gut barrier. SAL and CC combination reversed the translocation of gut microbiota, reduced systemic inflammatory response. Conclusion: SAL and CC combination can reduce cerebral ischemia-reperfusion injury by regulating gut microbiota and brain-gut barrier.