bluuebunny/crossref_metadata_2025_split huggingface dataset:
miRNA‐34c‐5p targets Fra‐1 to inhibit pulmonary fibrosis induced by silica through p53 and PTEN/PI3K/Akt signaling pathway
Authors: Pang Xinru , Shi Haojun , Chen Xiaoshu , Li Chao , Shi Bin , Yeo Abrey J. , Lavin Martin F. , Jia Qiang , Shao Hua , Zhang Juan , Yu Gongchang 
Published: May 2022

AbstractSilica dust particles are representative of air pollution and long‐term inhalation of silicon‐containing dust through the respiratory tract can cause pulmonary fibrosis. Epithelial‐mesenchymal transformation (EMT) plays an important role in the development of fibrosis. This process can relax cell–cell adhesion complexes and enhance cell migration and invasion properties of these cells. Dysregulation of microRNA‐34c (miR‐34c) is highly correlated with organ fibrosis including pulmonary fibrosis. In this study, we found that miR‐34c‐5p could alleviate the occurrence and development of silica‐mediated EMT. Fos‐related antigen 1 was identified as a functional target of miR‐34c‐5p by bioinformatics analysis and the dual luciferase gene reporting assay. Importantly, chemically induced up‐regulation of hsa‐miR‐34c‐5p correlated inversely with the expression of Fra‐1 and further exploration found that the miR‐34c‐5p/Fra‐1 axis inhibits the activation of the phosphatase and tensin homolog deleted on chromosome 10/phosphatidylinositol‐4,5‐bisphosphate3‐kinase/protein kinase B (PTEN/PI3K/AKT) signaling pathway. In addition, through interaction with PTEN/p53 it inhibits the proliferation and migration of human bronchial epithelial cells stimulated by silica, and promotes cell apoptosis, thereby preventing EMT. This finding provides a promising biomarker for the diagnosis and prognosis of pulmonary fibrosis. Furthermore, overexpression of miR‐34c‐5p represents a potential therapeutic approach.
Co‐constructing Agency: Weaving Academic Discussion
Authors: Meston Heather M. , Phillips Galloway Emily , Barrack K. Addison 
Published: May 2022

AbstractAcademic discussion can create space for student voice and active negotiation of text meaning in the classroom. However, teachers hoping to use academic discussion may find it difficult to draw learners into these discussions if students do not feel a sense of agency—a particular concern for multilingual students or those designated as having limited or interrupted formal education, who may have shorter histories of participation in classroom discourse. In this article, we draw on observation, artifact, and interview data to explore an upper‐elementary classroom of newcomer multilingual learners, where reflexive academic discussion serves as a foundational element of exploring and developing students' agency. The authors present instructional approaches reading educators can use to weave together reflexive and text‐focused academic discussion in support of students' developing agency.
Towards age‐friendly municipalities: What are municipalities doing to support their ageing population with mental health problems?
Authors: Schichel Mignon C. P. , Veenstra Marja Y. , Kempen Gertrudis I. J. M. , de Vugt Marjolein E. , Verhey Frans R. J. 
Published: May 2022

AbstractMunicipalities in Western Europe have increasingly gained a responsibility to support their populations' healthy ageing, but are they ready to do so? In this case study, we are looking into the awareness, attitudes, capacities and mandates of 33 neighboring Belgian, Dutch, and German municipalities to support ageing people, and especially people with dementia, people with late‐life depression and informal caregivers. As part of the Senior Friendly Communities Interreg project, we conducted semi‐structured interviews with municipal policymakers and relevant stakeholders from May 2017 to January 2018. The results show that dementia was a more salient theme in municipality policies than depression; that informal caregivers were considered important but difficult for municipalities to identify; that there was a difference between how embedded these health themes were in policies versus in practice; and lastly, that conducting this assessment turned out to act as an intervention itself. We discuss the similarities and differences between the regions, and the context that needs to be taken into account for municipalities when becoming more age‐friendly. These results do not only provide an insight into the status quo of the participating municipalities with regard to becoming more age‐friendly but can provide a starting point for further development of policies in this area.
Protocol Buffers
Authors: Currier Chris 
Published: May 2022

AbstractProtocol Buffers (Protobufs) are discussed in this chapter, from creating one to analyzing the data. This particular serialization format, originally developed by Google, is used in various apps.We discuss creating a protocol buffer and adding data through Python step by step. This provides a better understanding of how and why protocol buffers are formed and used. We also clarify how to recognize and decode them during a forensic examination.
Genetic risk of type 2 diabetes modifies the effects of a lifestyle intervention aimed at the prevention of gestational and postpartum diabetes
Authors: Huvinen Emilia , Lahti Jari , Klemetti Miira M. , Bergman Paula H. , Räikkönen Katri , Orho-Melander Marju , Laivuori Hannele , Koivusalo Saila B. 
Published: May 2022

Abstract
Aims/hypothesis
The aim of this study was to assess the interaction between genetic risk and lifestyle intervention on the occurrence of gestational diabetes mellitus (GDM) and postpartum diabetes.

Methods
The RADIEL study is an RCT aimed at prevention of GDM and postpartum diabetes through lifestyle intervention. Participants with a BMI ≥30 kg/m2 and/or prior GDM were allocated to intervention and control groups before pregnancy or in early pregnancy. The study visits took place every 3 months before pregnancy, once in each trimester, and at 6 weeks and 6 and 12 months postpartum. We calculated a polygenic risk score (PRS) based on 50 risk variants for type 2 diabetes.

Results
Altogether, 516 participants provided genetic and GDM data. The PRS was associated with higher glycaemic levels (fasting glucose and/or HbA1c) and a lower insulin secretion index in the second and third trimesters and at 12 months postpartum, as well as with a higher occurrence of GDM and glycaemic abnormalities at 12 months postpartum (n = 356). There was an interaction between the PRS and lifestyle intervention (p=0.016 during pregnancy and p=0.024 postpartum) when analysing participants who did not have GDM at the first study visit during pregnancy (n = 386). When analysing women in tertiles according to the PRS, the intervention was effective in reducing the age-adjusted occurrence of GDM only among those with the highest genetic risk (OR 0.37; 95% CI 0.17, 0.82). The risk of glycaemic abnormalities at 12 months postpartum was reduced in the same group after adjusting additionally for BMI, parity, smoking and education (OR 0.35; 95% CI 0.13, 0.97).

Conclusions/interpretation
Genetic predisposition to diabetes modifies the response to a lifestyle intervention aimed at prevention of GDM and postpartum diabetes. This suggests that lifestyle intervention may benefit from being tailored according to genetic risk.

Clinical trial registration
ClinicalTrials.gov identifier: NCT01698385

Graphical abstract
Social Interaction is Unnecessary for Hindgut Microbiome Transmission in Honey Bees: The Effect of Diet and Social Exposure on Tissue-Specific Microbiome Assembly
Authors: Anderson Kirk E. , Ricigliano Vincent A. , Copeland Duan C. , Mott Brendon M. , Maes Patrick 
Published: May 2022

AbstractHoney bees are a model for host–microbial interactions with experimental designs evolving towards conventionalized worker bees. Research on gut microbiome transmission and assembly has examined only a fraction of factors associated with the colony and hive environment. Here, we studied the effects of diet and social isolation on tissue-specific bacterial and fungal colonization of the midgut and two key hindgut regions. We found that both treatment factors significantly influenced early hindgut colonization explaining similar proportions of microbiome variation. In agreement with previous work, social interaction with older workers was unnecessary for core hindgut bacterial transmission. Exposure to natural eclosion and fresh stored pollen resulted in gut bacterial communities that were taxonomically and structurally equivalent to those produced in the natural colony setting. Stressed diets of no pollen or autoclaved pollen in social isolation resulted in decreased fungal abundance and bacterial diversity, and atypical microbiome structure and tissue-specific variation of functionally important core bacteria. Without exposure to the active hive environment, the abundance and strain diversity of keystone ileum species Gilliamella apicola was markedly reduced. These changes were associated with significantly larger ileum microbiotas suggesting that extended exposure to the active hive environment plays an antibiotic role in hindgut microbiome establishment. We conclude that core hindgut microbiome transmission is facultative horizontal with 5 of 6 core hindgut species readily acquired from the built hive structure and natural diet. Our findings contribute novel insights into factors influencing assembly and maintenance of honey bee gut microbiota and facilitate future experimental designs.
Quantitative thrombus characteristics on thin-slice computed tomography improve prediction of thrombus histopathology: results of the MR CLEAN Registry
Authors: Hund Hajo , Boodt Nikki , Arrarte Terreros Nerea , Taha Aladdin , Marquering Henk A. , van Es Adriaan C. G. M. , Bokkers Reinoud P. H. , Lycklama à Nijeholt Geert J. , Majoie Charles B.L.M. , Dippel Diederik W.J. , Lingsma Hester F. , van Beusekom Heleen M. M. , van der Lugt Aad ,   on behalf of the MR CLEAN Registry Investigators
Published: May 2022

Abstract
Objectives
Thrombus computed tomography (CT) characteristics might be used to assess histopathologic thrombus composition in patients treated with endovascular thrombectomy (EVT) for acute ischemic stroke (AIS). We aimed to assess the variability in thrombus composition that could be predicted with combined thrombus CT characteristics.

Methods
Thrombi of patients enrolled in the MR CLEAN Registry between March 2014 and June 2016 were histologically analyzed with hematoxylin-eosin staining and quantified for percentages of red blood cells (RBCs) and fibrin/platelets. We estimated the association between general qualitative characteristics (hyperdense artery sign [HAS], occlusion location, clot burden score [CBS]) and thrombus composition with linear regression, and quantified RBC variability that could be explained with individual and combined characteristics with R2. For patients with available thin-slice (≤ 2.5 mm) imaging, we performed similar analyses for general and quantitative characteristics (HAS, occlusion location, CBS, [relative] thrombus density, thrombus length, perviousness, distance from ICA-terminus).

Results
In 332 included patients, the presence of HAS (aβ 7.8 [95% CI 3.9–11.7]) and shift towards a more proximal occlusion location (aβ 3.9 [95% CI 0.6–7.1]) were independently associated with increased RBC and decreased fibrin/platelet content. With general characteristics, 12% of RBC variability could be explained; HAS was the strongest predictor. In 94 patients with available thin-slice imaging, 30% of RBC variability could be explained; thrombus density and thrombus length were the strongest predictors.

Conclusions
Quantitative thrombus CT characteristics on thin-slice admission CT improve prediction of thrombus composition and might be used to further guide clinical decision-making in patients treated with EVT for AIS in the future.

Key Points
• With hyperdense artery sign and occlusion location, 12% of variability in thrombus RBC content can be explained.
• With hyperdense artery sign, occlusion location, and quantitative thrombus characteristics on thin-slice (≤ 2.5 mm) non-contrast CT and CTA, 30% of variability in thrombus RBC content can be explained.
• Absolute thrombus density and thrombus length were the strongest predictors for thrombus composition.
Model droplet formation in extensional filament stretching within a Filament Extension Atomizer
Authors: Neukötter Moritz , Jesinghausen Steffen , Schmid Hans-Joachim 
Published: May 2022

AbstractFurther innovation in the field of selective laser sintering (SLS) is strongly connected to the availability of new materials since the market is dominated by polyamide 12 (>90%). The aim of this publication is to develop a descriptive model for the droplet formation process in a Filament Extension Atomizer to predict the applicability to exploit further polymers for the SLS process. The feasibility was tested, investigated and characterized using a “Dripping out of a nozzle” setup for uniaxial extension. The droplet formation process was then observed via high-speed camera imaging and classified for certain parameters. The experiments were carried out using semi-diluted polyethylene oxide (600–4000 kg/mol), glycerol and water solutions as model fluids. Driven by the Plateau-Rayleigh instability, different types of spherical droplets were observed and various droplet formation mechanisms demonstrated and analyzed. Based on the experimental results, a predictive model is derived to describe various essential parameters.
ATRT–SHH comprises three molecular subgroups with characteristic clinical and histopathological features and prognostic significance
Authors: Federico Aniello , Thomas Christian , Miskiewicz Katarzyna , Woltering Niklas , Zin Francesca , Nemes Karolina , Bison Brigitte , Johann Pascal D. , Hawes Debra , Bens Susanne , Kordes Uwe , Albrecht Steffen , Dohmen Hildegard , Hauser Peter , Keyvani Kathy , van Landeghem Frank K. H. , Lund Eva Løbner , Scheie David , Mawrin Christian , Monoranu Camelia-Maria , Parm Ulhøi Benedicte , Pietsch Torsten , Reinhard Harald , Riemenschneider Markus J. , Sehested Astrid , Sumerauer David , Siebert Reiner , Paulus Werner , Frühwald Michael C. , Kool Marcel , Hasselblatt Martin 
Published: May 2022

AbstractAtypical teratoid/rhabdoid tumor (ATRT) is an aggressive central nervous system tumor characterized by loss of SMARCB1/INI1 protein expression and comprises three distinct molecular groups, ATRT–TYR, ATRT–MYC and ATRT–SHH. ATRT–SHH represents the largest molecular group and is heterogeneous with regard to age, tumor location and epigenetic profile. We, therefore, aimed to investigate if heterogeneity within ATRT–SHH might also have biological and clinical importance. Consensus clustering of DNA methylation profiles and confirmatory t-SNE analysis of 65 ATRT–SHH yielded three robust molecular subgroups, i.e., SHH-1A, SHH-1B and SHH-2. These subgroups differed by median age of onset (SHH-1A: 18 months, SHH-1B: 107 months, SHH-2: 13 months) and tumor location (SHH-1A: 88% supratentorial; SHH-1B: 85% supratentorial; SHH-2: 93% infratentorial, often extending to the pineal region). Subgroups showed comparable SMARCB1 mutational profiles, but pathogenic/likely pathogenic SMARCB1 germline variants were over-represented in SHH-2 (63%) as compared to SHH-1A (20%) and SHH-1B (0%). Protein expression of proneural marker ASCL1 (enriched in SHH-1B) and glial markers OLIG2 and GFAP (absent in SHH-2) as well as global mRNA expression patterns differed, but all subgroups were characterized by overexpression of SHH as well as Notch pathway members. In a Drosophila model, knockdown of Snr1 (the fly homologue of SMARCB1) in hedgehog activated cells not only altered hedgehog signaling, but also caused aberrant Notch signaling and formation of tumor-like structures. Finally, on survival analysis, molecular subgroup and age of onset (but not ASCL1 staining status) were independently associated with overall survival, older patients (> 3 years) harboring SHH-1B experiencing relatively favorable outcome. In conclusion, ATRT–SHH comprises three subgroups characterized by SHH and Notch pathway activation, but divergent molecular and clinical features. Our data suggest that molecular subgrouping of ATRT–SHH has prognostic relevance and might aid to stratify patients within future clinical trials.
Robotic-assisted minimally invasive Ivor Lewis esophagectomy within the prospective multicenter German da Vinci Xi registry trial
Authors: Egberts Jan-Hendrik , Welsch Thilo , Merboth Felix , Korn Sandra , Praetorius Christian , Stange Daniel E. , Distler Marius , Biebl Matthias , Pratschke Johann , Nickel Felix , Müller-Stich Beat , Perez Daniel , Izbicki Jakob R. , Becker Thomas , Weitz Jürgen 
Published: May 2022

Abstract

Purpose
Robotic-assisted minimally invasive esophagectomy (RAMIE) has become one standard approach for the operative treatment of esophageal tumors at specialized centers. Here, we report the results of a prospective multicenter registry for standardized RAMIE.

Methods
The German da Vinci Xi registry trial included all consecutive patients who underwent RAMIE at five tertiary university centers between Oct 17, 2017, and Jun 5, 2020. RAMIE was performed according to a standard technique using an intrathoracic circular stapled esophagogastrostomy.

Results
A total of 220 patients were included. The median age was 64 years. Total minimally invasive RAMIE was accomplished in 85.9%; hybrid resection with robotic-assisted thoracic approach was accomplished in an additional 11.4%. A circular stapler size of ≥28 mm was used in 84%, and the median blood loss and operative time were 200 (IQR: 80–400) ml and 425 (IQR: 335–527) min, respectively. The rate of anastomotic leakage was 13.2% (n=29), whereas the two centers with >70 cases each had rates of 7.0% and 12.0%. Pneumonia occurred in 19.5% of patients, and the 90-day mortality was 3.6%. Cumulative sum analysis of the operative time indicated the end of the learning curve after 22 cases.

Conclusions
High-quality multicenter registry data confirm that RAMIE is a safe procedure and can be reproduced with acceptable leak rates in a multicenter setting. The learning curve is comparably low for experienced robotic surgeons.
Long-term tracking and quantification of individual behavior in bumble bee colonies
Authors: Smith Matthew A.-Y. , Easton-Calabria August , Zhang Tony , Zmyslony Szymon , Thuma Jessie , Cronin Kayleigh , Pasadyn Cassandra L. , de Bivort Benjamin L. , Crall James D. 
Published: May 2022

AbstractSocial insects are ecologically dominant and provide vital ecosystem services. It is critical to understand collective responses of social insects such as bees to ecological perturbations. However, studying behavior of individual insects across entire colonies and across timescales relevant for colony performance (i.e., days or weeks) remains a central challenge. Here, we describe an approach for long-term monitoring of individuals within multiple bumble bee (Bombus spp.) colonies that combines the complementary strengths of multiple existing methods. Specifically, we combine (a) automated monitoring, (b) fiducial tag tracking, and (c) pose estimation to quantify behavior across multiple colonies over a 48 h period. Finally, we demonstrate the benefits of this approach by quantifying an important but subtle behavior (antennal activity) in bumble bee colonies, and how this behavior is impacted by a common environmental stressor (a neonicotinoid pesticide).
Interprofessional differences in preoperative planning—the individualist surgeon
Authors: Haak Fabian , Poletti Tino , Grossman Nir , von Holzen Urs , Bolli Martin , Klasen Jennifer M. , Glass Tracy , von Strauss und Torney Marco 
Published: May 2022

Summary
Background
Perioperative processes have a great impact on the quality of surgery. In a high-risk surrounding, proper planning and communication is of upmost importance. We have identified other professions next to surgery with comparable circumstances and conducted a survey to elaborate on the impact of perioperative processes.

Objective
To identify standards in preoperative planning in high-risk professions and determine possible shortcomings in surgical practice.

Methods
Two surveys were constructed and distributed to surgeons, mountain guides, and soldiers. Questions were designed to investigate preoperative planning behavior and compare the different professions.

Results
Nearly every participant (97%) agreed to the fact that preoperative planning helps to avoid complications. Most surgeons agreed that the preoperative and postoperative phase of care had the greatest ability to improve overall quality of care. The opinions about planning were divided. The minority of surgeons agreed to the importance of sharing a plan preoperatively. Soldiers were the profession with the highest rate of plan sharing.

Conclusion
The readiness to communicate varies between professions and is lowest for surgeons. Missing standardization of procedures and the surgeon’s ego might be explanations for this behavior. Interventions to overcome this shortcoming, like the preoperative team timeout, have already been implemented but further improvements are needed.
Criminal Justice Involvement after Release from Prison following Exposure to Community Mental Health Services among People Who Use Illicit Drugs and Have Mental Illness: a Systematic Review
Authors: Stewart Ashleigh C. , Cossar Reece D. , Quinn Brendan , Dietze Paul , Romero Lorena , Wilkinson Anna L. , Stoové Mark 
Published: May 2022

AbstractIllicit drug use and mental illness are common among people in prison and are associated with higher rates of reoffending and reimprisonment. We conducted a systematic review, searching MEDLINE, Embase, and PsycINFO to January 10, 2022, for studies reporting criminal justice involvement following exposure to community mental health services among people released from jail or prison who use illicit drugs and have mental illness. Our search identified 6954 studies; 13 were eligible for inclusion in this review. Studies were separated into three broad categories based on community mental health service type. Eleven of 13 studies reported a reduction in criminal justice involvement among participants exposed to community mental health services compared to a comparison group. Findings indicate a need to expand and improve integration and referral mechanisms linking people to community mental health services after jail or prison release, alongside a need for tailored programs for individuals with complex illicit drug use and mental health morbidities.
Architekturpsychologische Gestaltung von Arbeitsumwelten
Authors: Becker Matthias , Graf-Szczuka Karola , Wieschrath Sangeetha 
Published: May 2022

ZusammenfassungDieser Beitrag der Zeitschrift „Gruppe. Interaktion. Organisation. (GIO)“ verfolgt das Ziel, eine Übersicht über den Forschungsstand zu anwendungsorientierter (Neu‑)Gestaltung von Arbeitsumwelten aus dem Bereich der Architekturpsychologie zu geben.Durch die Digitalisierung und die zunehmende Ortsunabhängigkeit von Arbeit werden Arbeitsumwelten in der Organisation immer mehr zu einem Dreh- und Angelpunkt der organisationalen Zusammenarbeit. Solche „organizational hubs“ sollten daher zielgerichtet gestaltet werden. Welche architektonischen Gestaltungselemente sind für diesen Kontext relevant und wie lässt sich deren Wirkungsweise theoretisch begründen? Das Review liefert für diesen spezifischen Anwendungsfall geeignet erscheinende Ansätze. Ein besonderer Fokus liegt dabei auf den Auswirkungen von Gestaltungselementen auf das Wohlbefinden und die Leistungsfähigkeit von Beschäftigten. Auf dieser Grundlage erfolgt eine knappe Zusammenstellung der bestehenden, heterogenen Befundlage zu einzelnen architekturpsychologischen Gestaltungselementen. Als Ergebnis der eingegrenzten Literatur-Übersicht lässt sich ein Forschungsdefizit bezüglich der Integration, Interaktionen und Gewichtung der einzelnen Elemente identifizieren, welches zukünftig in Forschung und Praxis adressiert werden muss.