bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological and hematological profile of traumatic spinal cord injury: a retrospective study
Authors: Li Fang , Wei Changkun , Huo Su , Song Weiqun 
Published: August 2022

Abstract
Study design: A retrospective hospital-based study.Objective: To describe the epidemiological features and identify hematological indicators with prognostic value for function of traumatic spinal cord injury (TSCI) in Beijing, China.Setting: Xuanwu Hospital of Capital Medical University, Beijing.Methods: Routine blood and clinical data of TSCI patients who admitted to Xuanwu Hospital of Capital Medical University from 2015 to 2021 were collected for detailed investigation.Results: In all, 129 cases were identified. The most commonly affected age group was 46-60 years, and male-to-female ratio was 2.5:1. The leading cause was low fall (50.4%), followed by transport (25.6%). The most common injury site was the cervical spinal cord, especially C5, accounting for 86.0%. The proportion of TSCI patients receiving rehabilitation treatment after acute management was 27.2%. In addition, hemoglobin (Hb) concentrations in TSCI patients with increased Barthel index (BI) scores were significantly higher than those without increased BI scores (P＜0.05). Conclusion: Prevention strategies for TSCI should mainly target 46-60 age group, males and low fall. The results of this study will serve as a basis for further studies on TSCI, at least in Beijing. The preventive programs should be designed according to the injury characteristics. Additionally, higher Hb concentration in peripheral blood is closely related to the improvement of activities of daily living (ADL) in TSCI patients, but further detailed research is urgently needed.
Patient-Reported Outcome Measures compared to Clinician Reported Outcomes regarding incontinence and erectile dysfunction in localized prostate carcinoma after Robot Assisted Radical Prostatectomy: impact on management
Authors: Tillier CN , Boekhout AH , Veerman H , Wollersheim BM , Groen PAM , Poel HG van der , de Poll-Franse LV van 
Published: August 2022

Abstract
Purpose/ Background: To examine whether a discrepancy between patient reported outcomes (PROs) and clinician reported outcomes (CROs) impacts the management of urinary incontinence (UI) and erectile dysfunction (ED) after robot assisted radical prostatectomy (RARP). Methods: Up to 1 year after RARP, UI and ED recovery of 312 men with localized and locally advanced prostate cancer were assessed using the International Consultation Incontinence Questionnaire Short Form (ICIQ-SF) and the International Index of Erectile Function (IIEF-EF) and CROs by interview. Discrepancies between PROs and CROs were studied in light of treatment offered and management.Results: The ICIQ-SF Score matched with CROs in all sum score categories except in ICIQ sum score 6-12; here the UI was underreported by clinicians in 58% and 59% of patients at 8 and 12 months (p<.001). Furthermore, at 8 and 12 months postoperatively, clinicians underreported UI in 29% and 23% of patients with ICIQ score 13-18 (p<.001). The clinician significantly over-reported the recovery of erectile function (“normal erection”) (p<.001), especially in men with IIEF-EF sum score 6-16. Independently of ICIQ-SF/IIEF-EF scores, discrepancy between PROs and CROs did not affect rate of health care offered to patients. Conclusions: This is to our knowledge the first study that compared the PROs with clinician reported functional outcomes and the impact of discrepancies on the management of side effects of RARP in prostate cancer. Observed discrepancies between the PROs and CROs did not affect offered management and counseling of UI and ED.
Sodium tanshinone IIA sulfonate protects Myocardium from Ischemia/ Reperfusion Injury in Mice via AKT- and SIRT1/TLR4-related Mechanisms
Authors: Ding Hua-Sheng , Qu Fu-Ji , Li Qiu-Jian , Luo Zhi-Hui , Peng Yi , Wang Feng-Yuan , Zhong Peng , Huang Yan , Liu Xiao-Xiong 
Published: August 2022

Abstract
Background
Cardiac ischemia-reperfusion (I/R) injury is highly related to ischemic heart disease which is the main cause of death worldwide. However, no standard therapy for I/R is available. Sodium tanshinone IIA sulfonate (STS) has been demonstrated to play an important role in myocardial I/R injury, however its therapeutic effects and potential mechanism remain still unclear. This study was aimed to investigate the potential protective effects of STS on myocardial I/R injury.
Methods
STS were injected intraperitoneally at 30 mg/kg per mouse beginning 10 days before I/R surgery. Histological analyses and markers, echocardiographic parameters, TTC staining were examined to assess myocardial injury.
Results
Compared with I/R mice, I/R in STS-administered mice leaded to diminished myocardial infarct size, apoptosis, and toll-like receptor 4 (TLR4) expression. In cultured cardiomyocytes, STS inhibits apoptosis and production of TLR4, IL-6. Dominant negative AKT reversed the inhibitory effect of STS on apoptosis, but had no effect on the inhibitory effect of STS on IL-6 production. STS induces SIRT1/TLR4-dependent anti-inflammation in cardiomyocytes, and inhibition of SIRT1 by siSIRT1 reverses the inhibitory effect of STS on inflammation and infarct size.
Conclusion
Our study suggest that STS can relieve heart I/R injury through AKT and SIRT1/TLR4-dependent mechanisms in vivo and vitro.
Wetland conservation and management practices in Rubanda District, South-Western Uganda
Authors: Turyasingura Benson , Alex Saturday , Hirwa Hubert , Mohammed Fatima Sule 
Published: August 2022

Abstract
Worldwide, wetlands cover about 9% of the land surface and are recognized as bio-networks that offer living prospects when managed properly. Despite the present resource management regime, many wetlands in Uganda are being degraded due to mining, construction, agricultural and industrial activity, and little attention has been paid to the effectiveness of wetland conservation. The goal of this research was to evaluate Nyamuriro wetland conservation and management practices in Rubanda District, Uganda. The study was guided by specific objectives, which included investigating the management approaches used in Nyamuriro wetland conservation, identifying the management challenges preventing Nyamuriro wetland conservation, and evaluating sustainable solutions to the challenges preventing Nyamuriro wetland conservation. The study employed a descriptive cross-sectional research design that included qualitative and quantitative data gathering and analysis techniques. A total of 109 people participated in the study using simple random and purposive sampling approaches. In this study, data was collected using questionnaires, key informant interviews, and observational approaches. Most respondents strongly agree that wetland restoration is the best management approach for Nyamuriro wetland conservation (Mean = 4.87, SD = 0.547). Corruption was the most significant hindrance to the conservation of Nyamuriro wetland among the management problems (Mean = 3.41, SD = 0.467). Overall, the data showed that there was a significant positive association between conservation management techniques but not between viable Nyamuriro wetland conservation measures. The study recommended that strict laws and procedures should be put in place to ease restrictions on wetland conservation.
Convergent, slightly misoriented crystals toughen corals and seashells
Authors: Gilbert Pupa , Lew Andrew , Stifler Cayla , Tits Alexandra , Schmidt Connor , Luffey Emily , Scholl Andreas , Cantamessa Astrid , Muller Laura , Delaunois Yann , Compère Philippe , Ruffoni Davide , Buehler Markus 
Published: August 2022

Abstract
The hardest and toughest tissues formed by living organisms are organic-mineral composites termed biominerals 1,2. When they are crystalline, their mesostructure includes the nano- and micro-scale crystallite size, shape, arrangement, and orientation. Mesostructures vary enormously across marine CaCO3 biominerals (aragonite, vaterite, calcite) because they result from divergent evolution: biominerals were formed long after organisms diverged from one another 3,4. Despite such diversity, CaCO3 marine biominerals share a convergent character: adjacent crystals are similarly oriented 5-32. The reason for such convergence is unclear. Here, we show with quantitative, precise measurements at the nanoscale that the slight misorientation is consistently between 1°-40° in diverse biominerals. Can this slight misorientation confer a desirable materials property and therefore an evolutionary advantage to the forming organisms? We test and confirm this hypothesis with nanoindentation in diverse biominerals, geologic aragonite, and in abiotic, slightly misoriented, synthetic spherulites. Molecular dynamics (MD) simulations of bicrystals reveal that aragonite, vaterite, calcite, exhibit toughness peaks when they are misoriented by 10°, 20°, 30°, respectively, demonstrating that slight misorientation alone increases crack deflection and therefore fracture toughness. Slight misorientation, along with other previously known and co-existing toughening mechanisms, was selected repeatedly and convergently, during the course of evolution, to postpone fracture and thus provide organisms with competitive advantage. We anticipate slight misorientation-toughening to be a starting point for more sophisticated materials synthesis and additive manufacturing in many fields. Compared to previously known toughening mechanisms, in fact, the advantages of slight misorientation are that it can and does occur in synthetic materials, it requires one material only and no specific top-down architecture, it is easily achieved by self-assembly of organic molecules (e.g. aspirin, chocolate), polymers, metals, and ceramics 29 well beyond biominerals.
IoT-Based Data-Driven Predictive Maintenance Relying on Fuzzy System and Artificial Neural Networks
Authors: Aboshosha Ashraf , Haggag Ayman , George Neseem , Hamed Hisham A. 
Published: August 2022

Abstract
Industry 4.0 technologies need to plan reactive maintenance and Preventive Maintenance (PM) strategies for their production machines. However, preventive maintenance cannot predict the future faults or conditions of the machine components in advance to prevent halting the production cycle. This study aims to use a Predictive Maintenance (PdM) technology with communication technologies to counter these problems. Sensors Information Modeling (SIM) and the Internet of Things (IoT) have the potential to improve the efficiency of industrial production machines maintenance management. They can provide a better maintenance strategy utilizing a data-driven predictive maintenance planning framework based on our proposed SIM and IoT technologies. Data is collected and integrated with the proposed (SIM) models and the IoT network. The proposed system consists of the data entry form that contains all the measurements, notes of events that are difficult to electronically monitor, and the credibility of data measured by sensors. The status and performance of the machine are continuously being monitored as they consciously vary according to the operating conditions. Data collection from the SIM models, and IoT network are integrated in a software framework that consists of, (1) condition and performance monitoring and faults module, (2) machine condition evaluation module, (3) failure prediction module, (4) maintenance decision module. Machine learning algorithms and artificial neural networks are used to detect the healthy or faulty operating conditions and predict probability of failures, evaluate the conditions of the machine components and estimate the remaining lifetime. To verify the feasibility of our approach, our proposed framework is applied to a corrugated board production factory real industrial environment. The results show that, updated data obtained from the information layer with machine learning algorithms in the application the layer can effectively predict the future state of the machine components to make appropriate maintenance decisions.
Lactobacillus fermentum (MTCC-5898) based fermented whey renders prophylactic action against colitis by strengthening the gut barrier function and maintaining immune homeostasis
Authors: Kaur Harpreet , Gupta Taruna , Kapila Suman , Kapila Rajeev 
Published: August 2022

Abstract
Nutritional intervention using probiotic fermented dairy product has emerged as a promising therapeutic strategy to curb inflammatory bowel diseases. The present study was aimed to investigate the prophylactic capability of probiotic Lactobacillus fermentum (LF:MTCC-5898) fermented whey on impaired barrier function in DSS induced mice. Probiotic fermented whey (PFW) consumption improved the symptoms of colitis-associated with intestinal inflammation by significantly (p < 0.01) diminishing the percent loss in body weight, disease activity index and spleen index with improvement in colon length and weight besides hematological and histopathological score. Likewise, pre-treatment with PFW improved the barrier integrity (p < 0.01) in contrast to leaky condition induced by DSS administration characterized with increased permeability of FITC-dextran (451 ± 225.1) across epithelium. PFW consumption also provided the gut immune protection that occurred via significant increase (p < 0.05) in TLR-2 expression and stimulated T-regulatory response by producing TGF-β (p < 0.01), to potently suppress (p < 0.01) induced inflammatory response markers (TNF-α, IL-4 and C-reactive protein). Consistently, in preventive way, PFW intake significantly enhanced (p < 0.05) immunoglobulin (sIgA) secretion and concomitantly restored the Occludin, ZO-1 (p < 0.01), Claudin-1(p < 0.05) transcriptional expression as compared to colitis mice. Additionally, immune-fluorescence further established the presence of intact actin cytoskeleton and tight junction proteins (claudin-1, occludin and ZO-1) after PFW consumption. Thus, PFW rectified the impaired and leaky barrier junctions not only through modulation of transcriptional expression of tight junction genes but also with reduced secretion of inflammatory mediators and helped in ameliorating the colitis. Hence, probiotic fermented whey could be used as potential prophylactic functional food in the prevention of gut ailments.
Fig latex inhibits the growth of pathogenic bacteria invading human diabetic wounds and accelerating the wound closure in diabetic mice
Authors: Salah Mohamed , Badr Gamal , Hetta Helal F. , Khalifa Walaa A. , Shoreit Ahmed A. 
Published: August 2022

Abstract
Impaired wound healing is one of the most critical complications associated with diabetes mellitus. Hence, novel and more effective natural therapeutic antibacterial agents are urgently needed and should be developed against the pathogenic bacteria inhabiting the diabetic wounds. Therefore, the current study aimed to investigate the impact of fig latex on the pathogenic bacteria and its ability to promote the healing process of diabetic wounds. The pathogenic bacteria were isolated from patients with diabetic wounds admitted at Assiut University hospital. Fig latex was collected from trees in Assiut regions, and its chemical compositions were analyzed using GC-MS. The anti-bacterial efficacy of the fig latex was assessed on the isolated bacteria. In vivo study to investigate the effect of fig latex on the diabetic wound healing was monitored using three mice groups: non-diabetic control, diabetic and diabetic treated with fig latex. The influence of fig latex on the expression levels of β-defensin-1, collagen formation, PECAM-1, CCL2 and ZO-1 were investigated. The GC-MS analysis demonstrated the presence of triterpenoids with more than 90% of total latex content. Furthermore, using streptozotocin (STZ)-induced diabetic mice model, topical treatment of diabetic wounded tissues with fig latex accelerated and improved the wound closure through increasing the expression levels of β-defensin-1, collagen, and PECAM-1 as compared to untreated diabetic wounds. Additionally, fig latex down-regulated the expression levels of ZO-1 and CCL2.
A comparative study of panoramic radiography and cone-beam computed tomography about the relationship of mandibular third molar an inferior alveolar canal
Authors: Jian Zhang , Xiaoyu Gao , Wenyi Zhang 
Published: August 2022

Abstract
Purpose To evaluate the accuracy of Panoramic radiography（PAN） by using CBCT to compare the anatomical relationship between the mandibular third molar and the inferior alveolar nerve canal (IAN).Methods The research sample included the imaging information of 953 MTMs and adjacent mandibular canals were studied. Statistical software SPSS25.0 was used, and chi-square test was used to analyze the correlation between demographic factors, type of obstruction, special imaging findings of PAN and IAN canal defect in CBCT. The factors related to IAN canal defect were evaluated by Logistic regression. Results Among the 953 study subjects, 757 cases of inferior alveolar nerve canal cortical bone were intact and 196 cases of IAN canal are defect. The differences between mandibular duct wall defects in the classification of MTM, the darkening of roots, the interruption of the white line, the deviation diversion of canal, and the narrowing of canal were statistically significant. In logistic regression analysis, P&G classification (mesial-distal), the darkening of roots, and the interruption of the white line were risk factors for mandibular duct wall defects. Conclusions The interruption of the white line, darkening of the root, and the P&G classification (mesial-distal) suggest an increased rate of IAN canal defects. The special imaging manifestations related to the absence of cortical bone in the mandibular canal wall in univariate analysis, this study still recommends that further detailed investigation of CBCT is recommended.
Genotyping of cerebrospinal fluid for leptomeningeal metastases in EGFR-mutated lung cancer
Authors: Geng Di , Niu Ruina , Li Jinghong , Guo Sanxing , Guo Qianqian , Huang Siyuan , Wang Yurong , Li Xingya 
Published: August 2022

Abstract
Background: Leptomeningeal metastasis (LM) is a detrimental complication of advanced lung cancer, more frequently occurring in patients harboring epidermal growth factor receptor (EGFR) mutation. Due to limited access to the leptomeningeal lesion, we explored the potential role of cerebrospinal fluid (CSF) as a source for liquid biopsy in LM patients with EGFR mutation.Materials and methods: The CSF samples from Lung cancer patients with LM were collected between June 2021 and August 2018 at First Affiliated Hospital of Zhengzhou University. Next-generation sequencing was performed to detect the mutations in EGFR genes, overall 38 patients detected with EGFR mutations were finally enrolled in further retrospectively clinical analyses.Results: CSF samples of 38 lung patients with LM harboring EGFR mutation were enrolled in this study.we found 20 cases of L858R of exon 21 and 14 cases of exon 19 deletion.Other EGFR mutations included EGFR exon 20 insertion(n=1),EGFR G719A of exon 18 (n=1) ,EGFR G719S of exon 18 (n=1), EGFR S768I of exon 20 and G719C of exon 18(n=1).CSF samples from 6 patients were collected before prescribing them TKIs.The most frequently detected concurrent genes in CSF was TP53 missense mutation(50%).CSF of 10 patients were obtained after failure to 1st TKI.The most frequently detected concurrent genes in CSF were TP53 missense mutation(40%,n=4),EGFR copy number amplification (40%,n=4) and NKX2-1 copy number gain(30.0%,n=3).Acquired resistance to 1st TKI of T790M mutation was detected in two patients. Similarly, the CSF samples from 22 patients were collected during their 3rd TKI therapy and the TP53 missense mutation was detected in 31.8% of patients followed by EGFR amplification (22.7%), STK11 deletions (18.2%) and MYC amplification (18.2%), while two patients harbored T790M mutation. Known mechanisms of acquired resistance to EGFR- 3rd TKIs were identified in 31.8% of cases. C797S and C797G mutation was found in three patients.Possible EGFR-independent resistant mechanism included PIK3CA missense mutation(4.5%),MET amplification(4.5%),CDK4 amplification(4.5%) and RET gene fusion(4.5%).In the 38 cases , average TMB was 8.4mutations/Mb (median 6.9 , range 1.1-23.83mutations/Mb) ,12 cases with more than 10 mutations/Mb. There were no significant difference of CSF TMB among the three group of patients who hadn’t received TKI, progressed on 1st TKI and progressed on 3rd TKI.Conclusion: CSF cfDNA provided comprehensive profiles of driver and resistance genes of lung cancer with LM harboring EGFR mutation, supporting the importance of CSF cfDNA as a liquid biopsy medium and guiding the choice of anti-tumor drugs.
Exosomal MicroRNAs modulate the cognitive function in fasudil treated APPswe/PSEN1dE9 transgenic (APP/PS1) mice model of Alzheimer's disease
Authors: Yan Yuqing , Gao Ye , Kumar Gajendra , Fang Qingli , Zhang Nianping , Yan Hailong , Zhang Yuna , Song Lijuan , Li Jiehui , Zheng Yucheng , Zhang Nan , Zhang Han-Ting , Ma Cungen 
Published: August 2022

Abstract
Alzheimer’s disease (AD) is characterized by cognitive impairment caused by the accumulation of beta-amyloid (Aβ) plaques and trans-synaptic spread of tau pathology. Exosome has emerged as key mediators for neuronal development, maintenance, and cellular communication. However, the molecular mechanism of exosomal miRNAs related to AD remains unknown. In the present study, APPswe/PSEN1dE9 transgenic (APP/PS1) mice (AD) were treated with vehicle (ADNS) and fasudil (ADF), whereas C57BL/6 (control) mice were treated with vehicle (WT). Cognitive function was assessed by Y-maze test and AD pathology was confirmed by immunostaining of Aβ plaque and phosphorylated tau. Exosomal RNAs from each mouse of the group were extracted, sequenced and analyzed. Our results showed amelioration of cognitive function, decreased Aβ plaque load, and phosphorylated tau protein after fasudil treatment. Exosomal miRNA analysis showed 3 miRNAs (mmu-let-7i-5p, mmu-miR-19a-3p, mmu-miR-451a) in the intersection of ADNS vs ADF and WT vs ADNS. GO annotation and KEGG pathway showed the target gene of miR-19a-3p are Pten and Tnf; mmu-miR-451a are Nsmaf, Gnai3 and Akt3. In conclusions, fasudil treatment improved cognitive function by regulating exosomal MicroRNAs (mmu-miR-451a and mmu-miR-19a-3p). These MicroRNAs could be potential biomarker of AD and therapeutic target for novel treatment for AD.
Certification as dysplasia unit and its impact on large loop electrosurgical excision (LEEP)
Authors: Hanczuk Tatjana , Weiss Martin , Henes Leon , Engler Tobias , Neis Felix , Henes Melanie 
Published: August 2022

Abstract
Purpose
This study evaluates the overall treatment indicators and outcomes of patients who underwent loop electrosurgical excision procedure (LEEP) at the Department of Women’s Health Tübingen and the impact of certification as a dysplasia unit on treatment quality.
Methods
Retrospective analysis of data from 1596 patients from 2013 to 2018 who underwent LEEP excision at the Department of Women’s Health Tübingen. Data of cytology, colposcopy, biopsy, LEEP histology, repeat LEEP histology and general characteristics were collected and analyzed descriptively.
Results
A total of 85.4% (1364) had CIN 2 + and 14.6% (232) had CIN 1 or normal findings on LEEP histology. The proportion of CIN 2 + excisions increased significantly from 82.4% in 2013 to 89% in 2018. The concordance of HSIL biopsy and LEEP histology was 89.1% in 2013 and 92.6% in 2018. In 2018, more biopsies and colposcopies were performed before excision. Complete resection (R0) was achieved in 88.3% of all excisions. R0 rates in patients with CIN 3 increased in 2014–2017 compared to 2013, resulting fewer Re-LEEP excisions and hysterectomies.
Conclusion
Certification as a dysplasia unit and the associated requirements have resulted an increase of quality for patients with cervical dysplasia undergoing LEEP. This was demonstrated by several treatment indicators such as the number of colposcopies and biopsies and treatment outcomes such as increased proportion of CIN 2 + excisions and R0 resections.