bluuebunny/crossref_metadata_2025_split huggingface dataset:
Site impacts nutrient translocation efficiency in intraspecies and interspecies miscanthus hybrids on marginal lands
Authors: Magenau Elena , Clifton‐Brown John , Awty‐Carroll Danny , Ashman Chris , Ferrarini Andrea , Kontek Mislav , Martani Enrico , Roderick Kevin , Amaducci Stefano , Davey Chris , Jurišić Vanja , Kam Jason , Trindade Luisa M. , Lewandowski Iris , Kiesel Andreas 
Published: July 2022

AbstractMiscanthus, a C4 perennial rhizomatous grass, is capable of growing in varied climates and soil types in Europe, including on marginal lands. It can produce high yields with low nutrient inputs when harvested after complete senescence. Senescence induction and rate depend on complex genetic, environmental, and management interactions. To explore these interactions, we analysed four miscanthus hybrids (two novel seed‐based hybrids, GRC 3 [Miscanthus sinensis × sinensis] and GRC 14 [M. sacchariflorus × sinensis]; GRC 15, a novel M. sacchariflorus × sinensis clone; and GRC 9, a standard Miscanthus × giganteus clone) in Italy, Croatia, Germany and the UK. Over all trial locations and hybrids, the average aboveground biomass of the 3‐year‐old stands in August 2020 was 15 t DM ha−1 with nutrient contents of 7.6 mg N g−1 and 14.6 mg K g−1. As expected, delaying the harvest until spring reduced overall yield and nutrient contents (12 t DM ha−1, 3.3 mg N g−1, and 5.5 mg K g−1). At lower latitudes, the late‐ripening M. sacchariflorus × sinensis GRC 14 and GRC 15 combined high yields with low nutrient contents. At the most elevated latitude location (UK), the early‐ripening M. sinensis × sinensis combined high biomass yields with low nutrient offtakes. The clonal Miscanthus × giganteus with intermediate flowering and senescence attained similar low nutrient contents by spring harvest at all four locations. Seasonal changes in yield and nutrient levels analysed in this study provide: (1) a first step towards recommending hybrids for specific locations and end uses in Europe; (2) crucial data for determination of harvest time and practical steps in the valorization of biomass; and (3) key sustainability data for life cycle assessments. Identification of trade‐offs resulting from genetic × environment × management interactions is critical for increasing sustainable biomass supply from miscanthus grown on marginal lands.
Improved anti‐solid tumor response by humanized anti‐podoplanin chimeric antigen receptor transduced human cytotoxic T cells in an animal model
Authors: Ishikawa Akihiro , Waseda Masazumi , Ishii Tomoko , Kaneko Mika K. , Kato Yukinari , Kaneko Shin 
Published: July 2022

AbstractRecently, research has been conducted with chimeric antigen receptor (CAR)‐T cells to improve efficacy against solid tumors. Humanized CAR improved the long‐term survival of CAR‐T cells in patients' peripheral blood, resulting in increased therapeutic efficacy. Therefore, the humanization of the CAR‐gene sequence is considered an effective method. Podoplanin (PDPN) is a glycosylated transmembrane protein that is highly expressed in solid tumors and is associated with poor prognosis in patients with cancer. Therefore, PDPN is considered a biomarker and good target for cancer treatment with CAR‐T cells. Previously, an anti‐PDPN CAR was generated from a conventional nonhumanized antibody—NZ‐1, the only anti‐PDPN antibody for which a CAR was produced. In this study, we investigated other anti‐PDPN CARs from the antibody NZ‐27, or humanized NZ‐1, to enhance the therapeutic potential of CAR‐T cells. The CAR signal intensity was enhanced by the efficient expression of CAR proteins on the T‐cell surface of NZ‐27 CAR‐T cells, which show tumor‐specific cytotoxicity, proinflammatory cytokine production, and anti‐tumor activity against PDPN‐expressing tumor xenografts in mice that were significantly better than those in nonhumanized NZ‐1 CAR‐T cells.
Influence of supplement administration of omega‐3 on the subcutaneous tissue response of endodontic sealers in Wistar rats
Authors: de Oliveira Pedro Henrique Chaves , Gomes Filho João Eduardo , Rodrigues Michael Júnio da Silva , da Silva Cristiane Cantiga , Cardoso Carolina da Barros Moraes , Cosme daSilva Leopoldo , Ervolino Edilson , Cintra Luciano Tavares Angelo 
Published: July 2022

AbstractAimNatural substances such as omega‐3 have been used in the medical field due to their numerous properties and, in particular, modulating effect on the systemic and local inflammatory processes. Thus, this study evaluated the influence of omega‐3 supplementation on the subcutaneous tissue response of endodontic sealers in Wistar Rats.MethodologyPolyethylene tubes were implanted in the subcutaneous tissue of 48 animals (one empty for control and three filled with Sealapex, AH Plus or Endofill). The animals were treated with omega‐3 (TO) or water (TW). Treatments started 15 days before implantation until euthanasia. After 5, 15 and 30 days (n = 8), animals were euthanized and polyethylene tubes and surrounding tissue were removed and processed for histological analysis. The inflammatory reaction was analysed by Haematoxylin and Eosin stain and immunolabelling for IL‐6 and TNF‐α. The collagen maturity was analysed by picrosirius red stain and calcium deposition by von Kossa stain and polarized light. Results were statistically analysed (p < .05).ResultsAmongst TW sealer groups, Endofill evoked a more intense inflammatory infiltrate compared with AH Plus and control in the 30‐day period (p = .009). However, in TO sealer groups, there was no difference amongst the sealers and control in all periods (p > .05). Comparing each sealer as a function of the supplementation with water or omega‐3, there are differences for Endofill (p = .001) and Sealapex (p = .005) in the 30‐day period, presenting lower inflammatory infiltrate in the animals treated with omega‐3. A higher percentage of immature fibres was observed at 15 and 30 days in the TO group, compared with the TW group (p < .05). The deposition of calcium particles was observed only by Sealapex in all periods, despite the supplementation procedure.ConclusionsOmega‐3 supplementation influence the tissue reactions of endodontic sealers, modulating inflammation, the immunolabelling of IL‐6 and TNF‐α, the repair process and it does not interfere with calcium deposition.
The attention‐based view: Review and conceptual extension towards situated attention
Authors: Brielmaier Christoph , Friesl Martin 
Published: July 2022

AbstractOver two decades ago, William Ocasio introduced the attention‐based view (ABV) of the firm with a powerful argument: firm‐level behaviour is the result of the situated distribution and allocation of managerial attention, embedded in broader organizational structures and the environmental context. ABV‐based research has received substantial and increasing scholarly attention, resulting in a complex and incoherent body of research. In order to address this issue, this paper takes stock of extant research on the ABV and consolidates key debates. Based on a systematic review of 173 articles, we synthesize existing research into a unifying framework. Drawing on this framework, we propose situated attention as a central theme for future research. We elaborate on four situational factors (materiality, social dynamics, temporality and what we call framing of the strategic setting), which may influence how actors’ attention is situated in the particular context.
Switches between biologics in patients with moderate‐to‐severe psoriasis: results from the French cohortPSOBIOTEQ
Authors: Curmin R. , Guillo S. , De Rycke Y. , Bachelez H. , Beylot‐Barry M. , Beneton N. , Chosidow O. , Dupuy A. , Joly P. , Jullien D. , Richard M.A. , Viguier M. , Sbidian E. , Paul C. , Mahé E. , Tubach F. ,   the PSOBIOTEQ Study Group
Published: July 2022

AbstractBackgroundBiologics are the cornerstone of treatment of patients with moderate‐to‐severe plaque psoriasis and switches between biologics are frequently needed to maintain clinical improvement over time.ObjectivesThe main purpose of this study was to describe precisely switches between biologics and how their pattern changed over time with the recent availability of new biologic agents.MethodsWe included patients receiving a first biologic agent in the Psobioteq multicenter cohort of adults with moderate‐to‐severe psoriasis receiving systemic treatment. We described switches between biologics with chronograms, Sankey and Sunburst diagrams, assessed cumulative incidence of first switch by competing risks survival analysis and reasons for switching. We assessed the factors associated with the type of switch (intra‐class – i.e. within the same therapeutic class ‐ vs. inter‐class) in patients switching from a TNF‐alpha inhibitor using multivariate logistic regression.ResultsA total of 2153 patients was included. The cumulative incidence of switches from first biologic was 34% at 3 years. Adalimumab and ustekinumab were the most prescribed biologic agents as first and second lines of treatment. The main reason for switching was loss of efficacy (72%), followed by adverse events (11%). Patients receiving a TNF‐alpha inhibitor before 2016 mostly switched to ustekinumab, whereas those switching in 2016 or after mostly switched to an IL‐17 inhibitor. Patients switching from a first‐line TNF‐alpha inhibitor before 2016 were more likely to switch to another TNF‐alpha inhibitor compared with patients switching since 2018. Patients switching from etanercept were more likely to receive another TNF‐alpha inhibitor rather than another therapeutic class of bDMARD compared with patients switching from adalimumab.ConclusionThis study described the switching patterns of biologic treatments and showed how they changed over time, due to the availability of the new biologic agents primarily IL‐17 inhibitors.
Comprehensive performance evaluation of stormwater management measures for sponge city construction: A case study in Gui'an New District, China
Authors: Yao Yichen , Hu Caihong , Liu Chengshuai , Yang Fan , Ma Bingyan , Wu Qiang , Li Xinan , Soomro Shan‐e‐hyder 
Published: July 2022

AbstractClimate change and rapid urbanization have increased pressure on drainage systems, posing new challenges to preventing and controlling urban waterlogging. In 2013, China proposed the Sponge City, a strategic measure for urban waterlogging control. This study quantifies the effects of stormwater management measures in runoff reduction for different levels of rainfall and conducts a one‐dimensional visual analysis of urban waterlogging risks. At the same time, the best cost‐effective scheme is determined based on life‐cycle cost, analytic hierarchy process, and regret decision theory. The results showed stormwater management measures could realize the function of runoff control and waterlogging prevention, especially under low precipitation. However, these measures were still not enough to eliminate waterlogging risk. Combined measures have stronger runoff control capabilities than single measures. Considering economic, environmental, and operational impacts comprehensively, the combined measures of bio‐retention (BR), permeable pavement (PP), and green roof (GR) were determined as the best cost‐effective scheme because of the lowest regret value. The proposed method is helpful to provide reference and decision‐making basis for the construction of sponge cities in the future.
Perception of atrial fibrillation symptoms: Impact on quality of life and treatment in older adults
Authors: Abu Hawa O. , Wang Weijia , Otabil Edith M. , Saczynski Jane S. , Mehawej Jordy , Mishra Ajay , Tisminetzky Mayra , Blanchard Gary , Gurwitz Jerry H. , Goldberg Robert J. , McManus David D. 
Published: July 2022

AbstractBackgroundIn managing older adults with atrial fibrillation (AF), their symptomatology impacts their well‐being and may inform treatment decision‐making. We examined AF symptom perception, its impact on quality of life (QoL), and its relation to treatment strategies in older adults with AF.MethodsData were obtained from older adults with AF enrolled in a multicenter study conducted at clinic sites in Massachusetts and Georgia between 2016 and 2018. Participants were stratified into three age groups: 65–74 (youngest–old), 75–84 (middle–old), and ≥85 (oldest). Perception of AF symptoms was assessed by participant self‐report during their clinic visit and at study enrollment by the Atrial Fibrillation Effect on Quality‐of‐Life Questionnaire which assessed cardiac‐specific and non‐specific, non‐cardiac AF symptoms and their impact on QoL. Treatment strategies (rate or rhythm control) utilized were ascertained from electronic medical records.ResultsAmong the 1184 participants (mean age 75 years, 48% women, 86% Non‐Hispanic White), 51% were aged 65–74 years, 36% were 75–84 years, and 13% were ≥ 85 years. The most commonly reported AF symptoms were non‐specific, non‐cardiac symptoms (fatigue, dyspnea, lightheadedness) with similar prevalence and impact on QoL in all age groups. Cardiac‐specific AF symptoms (palpitations, irregular heartbeat, pause in heart activity) were less prevalent, but most commonly reported by the youngest participants (65–74 years), who endorsed considerable impact of these symptoms on their QoL. Overall, those who reported experiencing any AF symptoms during their clinic visit were more likely to have received rhythm compared with rate control (OR: 1.56; 95% CI: 1.18–2.04) with similar findings for all age groups except those aged ≥85 years.ConclusionsOur findings suggest a high prevalence of non‐specific, non‐cardiac symptoms among older adults with AF and that cardiac‐specific AF symptoms may exert considerable impact on their QoL. The presence of any AF symptoms may drive more rhythm control in a majority of older adults.
Effect of leukoreduction on inflammation in critically ill dogs receiving red blood cell transfusions: A randomized blinded controlled clinical trial
Authors: Claus Melissa A. , Poh Denise , Smart Lisa , Purcell Sarah L. , Boyd Corrin J. , Sharp Claire R. 
Published: July 2022

AbstractBackgroundPrestorage leukoreduction of red blood cell (RBC) bags prevents accumulation of pro‐inflammatory mediators and experimentally attenuates post‐transfusion inflammation in healthy dogs. However, the effect of leukoreduction on post‐transfusion inflammation in critically ill dogs is unclear.HypothesisDogs transfused with leukoreduced (LR) RBC will have lower concentrations of leukocytes, interleukin (IL)‐6, IL‐8, monocyte chemoattractant protein‐1 (MCP‐1), and C‐reactive protein (CRP) within 24 hours of post‐transfusion compared to dogs transfused with nonleukoreduced (NLR) RBC.AnimalsSixty‐one RBC‐transfused dogs (LR = 34, NLR = 27).MethodsRandomized, blinded, controlled preliminary clinical trial. Blood bag processing was randomized to create identically appearing LR and NLR bags. Group allocation occurred with transfusion of the oldest compatible RBC bag. Blood samples were collected pretransfusion and at 8 and 24 hours post‐transfusion for leukocyte count, IL‐6, IL‐8, MCP‐1, and CRP. Data were analyzed on an intention‐to‐treat basis using linear mixed effects models. Significance was set at P < .05.ResultsNo significant differences were found between groups in concentrations of leukocytes (P = .93), IL‐6 (P = .99), IL‐8 (P = .75), MCP‐1 (P = .69), or CRP (P = .18) over time. Eleven LR dogs (32%) and 4 NLR dogs (15%) were euthanized in the hospital (P = .14). No natural deaths occurred.Conclusions and Clinical ImportanceNo differences in inflammation biomarker concentrations were detected over time between dogs transfused with LR or NLR RBC, but heterogeneity likely hampered the ability to detect a difference with this sample size. The novel randomization and enrollment protocol was successfully implemented across 2 participating institutions and will be easily scaled up for a future multicenter clinical trial.
Designing the learning of intraprofessional collaboration among medical residents
Authors: Looman Natasja , de Graaf Jacqueline , Thoonen Bart , van Asselt Dieneke , de Groot Esther , Kramer Anneke , Scherpbier Nynke , Fluit Cornelia 
Published: July 2022

AbstractBackgroundTo preserve quality and continuity of care, collaboration between primary‐care and secondary‐care physicians is becoming increasingly important. Therefore, learning intraprofessional collaboration (intraPC) requires explicit attention during postgraduate training. Hospital placements provide opportunities for intraPC learning, but these opportunities require interventions to support and enhance such learning. Design‐Principles guide the design and development of educational activities when theory‐driven Design‐Principles are tailored into context‐sensitive Design‐Principles. The aim of this study was to develop and substantiate a set of theory‐driven and context‐sensitive Design‐Principles for intraPC learning during hospital placements.MethodsBased on our earlier research, we formulated nine theory‐driven Design‐Principles. To enrich, refine and consolidate these principles, three focus group sessions with stakeholders were conducted using a Modified Nominal Group Technique. Next, two work conferences were conducted to test the feasibility and applicability of the Design‐Principles for developing intraPC educational activities and to sharpen the principles into a final set of Design‐Principles.ResultsThe theoretical Design‐Principles were discussed and modified iteratively. Two new Design‐Principles were added during focus group 1, and one more Design‐Principle was added during focus group 2. The Design‐Principles were categorised into three clusters: (i) Culture: building collaborative relations in a psychologically safe context where patterns or feelings of power dynamics between primary and secondary care physicians can be discussed; (ii) Connecting Contexts: making residents and supervisors mutually understand each other's work contexts and activities; and (iii) Making the Implicit Explicit: having supervising teams act as role models demonstrating intraPC and continuously pursuing improvement in intraPC to make intraPC explicit. Participants were unanimous in their view that the Design‐Principles in the Culture cluster were prerequisites to facilitate intraPC learning.ConclusionThis study led to the development of 12 theory‐driven and context‐sensitive Design‐Principles that may guide the design of educational activities to support intraPC learning during hospital placements.
Actin‐bundling protein fimbrin regulates pathogenicity via organizing F‐actin dynamics during appressorium development in Colletotrichum gloeosporioides
Authors: Zhang Yi , An Bang , Wang Wenfeng , Zhang Bei , He Chaozu , Luo Hongli , Wang Qiannan 
Published: July 2022

AbstractAnthracnose caused by Colletotrichum gloeosporioides leads to serious economic loss to rubber tree yield and other tropical crops. The appressorium, a specialized dome‐shaped infection structure, plays a crucial role in the pathogenesis of C. gloeosporioides. However, the mechanism of how actin cytoskeleton dynamics regulate appressorium formation and penetration remains poorly defined in C. gloeosporioides. In this study, an actin cross‐linking protein fimbrin homologue (CgFim1) was identified in C. gloeosporioides, and the knockout of CgFim1 led to impairment in vegetative growth, conidiation, and pathogenicity. We then investigated the roles of CgFim1 in the dynamic organization of the actin cytoskeleton. We observed that actin patches and cables localized at the apical and subapical regions of the hyphal tip, and showed a disc‐to‐ring dynamic around the pore during appressorium development. CgFim1 showed a similar distribution pattern to the actin cytoskeleton. Moreover, knockout of CgFim1 affected the polarity of the actin cytoskeleton in the hyphal tip and disrupted the actin dynamics and ring structure formation in the appressorium, which prevented polar growth and appressorium development. The CgFim1 mutant also interfered with the septin structure formation. This caused defects in pore wall overlay formation, pore contraction, and the extension of the penetration peg. These results reveal the mechanism by which CgFim1 regulates the growth and pathogenicity of C. gloeosporioides by organizing the actin cytoskeleton.
Videolaryngoscopes versus direct laryngoscopes in children: Ranking systematic review with network meta‐analyses of randomized clinical trials
Authors: de Carvalho Clístenes C. , Regueira Stéphanie L. P. A. , Souza Ana Beatriz S. , Medeiros Lucas M. L. F. , Manoel Marielle B. S. , da Silva Danielle M. , Santos Neto Jayme M. , Peyton James 
Published: July 2022

AbstractBackgroundVideolaryngoscopes improve tracheal intubation in adult patients, but we currently do not know whether they are similarly beneficial for children. We designed this ranking systematic review to compare individual video and direct laryngoscopes for efficacy and safety of orotracheal intubation in children.MethodsWe searched PubMed and five other databases on January 27, 2021. We included randomized clinical trials with patients aged ≤18 years, comparing different laryngoscopes for the outcomes: failed first intubation attempt; failed intubation within two attempts; failed intubation; glottic view; time for intubation; complications. In addition, we assessed the quality of evidence according to GRADE recommendations.ResultsWe included 46 studies in the meta‐analyses. Videolaryngoscopy reduced the risk of failed first intubation attempt (RR = 0.43; 95% CI: 0.31–0.61; p = .001) and failed intubation within two attempts (RR = 0.33; 95% CI: 0.33–0.33; p < .001) in children aged <1 year. Videolaryngoscopy also reduced the risk of major complications in both children aged <1 year (RR = 0.33; 95% CI: 0.12–0.96; p = .046) and children aged 0–18 years (RR = 0.40; 95% CI: 0.25–0.65; p = .002). We did not find significant difference between videolaryngoscopy and direct laryngoscopy for time to intubation in children aged <1 year (MD = −0.95 s; 95% CI: −5.45 to 3.57 s; p = .681), and children aged 0–18 years (MD = 1.65 s; 95% CI: −1.00 to 4.30 s; p = .222). Different videolaryngoscopes were associated with different performance metrics within this meta‐analysis. The overall quality of the evidence ranged from low to very low.ConclusionVideolaryngoscopes reduce the risk of failed first intubation attempts and major complications in children compared to direct laryngoscopes. However, not all videolaryngoscopes have the same performance metrics, and more data is needed to clarify which device may be better in different clinical scenarios. Additionally, care must be taken while interpreting our results and rankings due to the available evidence's low or very low quality.