bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequent pulse disturbances influence resistance and resilience in tropical marine microbial communities
Authors: Wijaya Winona , Suhaimi Zahirah , Xin’Er Cherlyn Chua , Sunil Rohan Shawn , Kolundžija Sandra , Bin Rohaizat Ahmad Muzakkir , Md. Azmi Norzarifah Binti , Hazrin-Chong Nur Hazlin , Lauro Federico M. 
Published: July 2022

ABSTRACTThe Johor Strait separates the island of Singapore from Peninsular Malaysia. A 1-kilometer causeway built in the early 1920s in the middle of the Strait effectively blocks water flowing to/from either side, resulting in low water turnover rates and build-up of nutrients in the inner Strait. We have previously shown that short-term rather than seasonal environmental changes influence microbial community composition in the Johor Strait. Here, we present a temporally-intensive study that uncovers the drivers keeping the microbial populations in check. We sampled the surface water at four sites in the inner Eastern Johor Strait every other day for two months, measured various water quality parameters, and analysed 16S amplicon sequences and flow-cytometry counts. We discovered that microbial community succession revolves around a common stable state resulting from frequent pulse disturbances. Among these, sporadic riverine freshwater input and regular tidal currents influence bottom-up controls including the availability of the limiting nutrient nitrogen and its biological release in readily available forms. From the top-down, marine viruses and predator bacteria (e.g. Saprospiraceae family) limit the proliferation of microbes in the water. Harmful algal blooms, which have been observed historically in these waters, may occur when there are gaps in the top-down and bottom-up controls. This study uncovers the complex interaction between these factors contributing to a low-resistance but high-resilience microbial community and speculate about the rare events that could lead to the occurrence of an algal bloom.
Extracellular Vesicles derived from Apis mellifera Royal Jelly promote wound healing by modulating inflammation and cellular responses
Authors: Alvarez S. , Contreras-Kallens P. , Aguayo S. , Ramirez O. , Vallejos C. , Ruiz J. , Carrasco-Gallardo E. , Troncoso-Vera S. , Morales B. , Schuh C.M.A.P. 
Published: July 2022

AbstractApis mellifera Royal Jelly (RJ) is a well-known remedy in traditional medicine around the world and its versatile effects range from antibacterial to anti-inflammatory properties and pro-regenerative properties. Several active compounds have been identified, however, the mechanisms of action still remain widely unknown. As a glandular product, RJ has been shown to contain a substantial number of extracellular vesicles (EVs) and in this study, we aimed to investigate the extent of involvement of RJEVs in wound healing associated effects. Molecular analysis of RJEVs verified the presence of important conserved exosomal markers such as CD63 and syntenin, as well as cargo molecules MRJP1, defensin-1 and jellein-3. RJEV internalization analysis demonstrated the involvement of membrane fusion as well as macropinocytosis or clathrin-dependent endocytosis into mammalian cells. Furthermore, RJEVs have demonstrated to modulate MSCs differentiation and secretome, as well as decrease LPS-induced inflammation in RAW 264.7 macrophages by blocking the MAPK pathway. In vivo studies confirmed anti-bacterial effects of RJEVs, and demonstrated an acceleration of wound healing in a splinted mouse model. Summarizing, this study suggests that RJEVs of potentially exosomal origin play a crucial role in the known effects of RJ by modulating the inflammatory phase and cellular response in wound healing.
Fluorogenic U-rich internal loop (FLURIL) tagging with bPNA enables intracellular RNA and DNA tracking
Authors: Liang Yufeng , Willey Sydney , Chung Yu-Chieh , Lo Yi-Meng , Miao Shiqin , Rundell Sarah , Tu Li-Chun , Bong Dennis 
Published: July 2022

ABSTRACTWe introduce herein a new strategy for intracellular RNA and DNA tracking that is robust, orthogonal and complementary to existing methods:FluorogenicU-RichInternalLoop (FLURIL) tagging with cell-permeable fluorophore-labeled bifacial Peptide Nucleic Acids (fbPNAs). Our approach uses an 8-nt (U4xU4) U-rich internal loop (URIL) in the RNA of interest (ROI) as a compact labeling site for fluorogenic triplex hybridization with a bPNA probe (~1 kD). FLURIL tagging thus replaces a 4 bp duplex stem with a labeled 4-base-triple hybrid stem of similar structure and mass. In contrast to existing strategies for RNA tracking, FLURIL tagging can be applied to internal, genetically encoded URIL RNA siteswith minimal structural perturbation, co-expression of protein-fusion labels or significant increase in molecular weight and steric bulk. We demonstrate effective FLURIL tagging of intracellular (HEK-293) RNAs, ribonucleoprotein (RNP) complexes and live cell (U2-OS) tracking of genomic loci. FLURIL tracking was internally validated by direct comparison with the most widely used live-cell RNA labeling method, MS2-labeling with MCP-HaloTag and Janelia Fluor dyes. In addition, FLURIL-tagging correctly reported on the endogenous RNP in HEK293 cells formed from TAR DNA binding protein 43 (TDP-43-tdTomato) and UG repeat RNA. The FLURIL strategy was also successfully applied to guide RNA (gRNA) in CRISPR-dCas complexes to enable live cell tracking of a low-copy number genomic locus (IDR3), internally benchmarked against MS2/HaloTag labeling of CRISPR-Sirius gRNA targeted to a proximal locus (IDR2). Notably, FLURIL-tagged IDR2 exhibited similar brightness as loci targeted by CRISPR-Sirius gRNA complexes, which bear 8-MS2 hairpins for protein labeling. Together, these experiments show that FLURIL tagging can simply and reliably track intracellular RNA, RNPs, and DNA, with a streamlined molecular footprint relative to other methods. Importantly, these data also indicate that FLURIL tagging is fully compatible with existing labeling methods without crosstalk and may be used to broaden the scope of intracellular RNA and DNA tracking.Abstract FigureScheme 1.FLURIL-tagging of RNAs with bPNA probes.(a) Triplex hybridization of a U-rich internal loop (URIL) with bPNA (blue) via base triple formation between the melamine base (M) and two uracil bases (inset). (b) General schematic of labeling strategy described herein. An RNA of interest is engineered to contain an URIL and expressed within the cell, with a fluorogenic bPNA probe introduced via cell culture media. Successful URIL targeting is reported by an increase in emission (green) and confirmed by a previously established RNA binding protein with a fluorescent protein (red) fusion.
Molecular dynamics study of phospholipid membrane electroporation induced by bipolar pulses with different intervals
Authors: Guo Fei , Zhou Jiong , Wang Ji , Qian Kun , Qu Hongchun 
Published: July 2022

AbstractIn this study, PM-EP induced by bipolar pulses with different intervals was investigated by all-atom molecular dynamics simulation. Firstly, PM-EP was formed during the positive pulse of 2ns and 0.5V/nm, then the effects of various intervals of 0, 1, 5, 10ns on PM-EP evolution were investigated, and the dynamic changes of different degrees of PM-EP induced by following negative pulses of 2ns and 0.5V/nm were analyzed. The elimination of the PM-EP during the interval of bipolar pulses were determined and it was related to the degrees of PM-EP and the time of intervals, then the degrees of PM-EP at the end of the intervals were classified and quantitatively defined, namely, Resealing, Destabilizing and Retaining state. These three states appeared due to the combined effect of both the preceding positive pulse and the interval. Furthermore, the evolution of PM-EP in resealing state under negative pulses was similar to that of positive pulses as evidenced by EP formation time and degree of PM-EP, the destabilizing state had the same trends as the resealing state except that the re-electroporation of phospholipid membrane appeared faster and larger degree of EP obtained with the same pulse exposure time. Regarding the retaining state, the negative pulses enhanced PM-EP with more profound water bridges, which can be considered as the effect of electric field superposition. These results can improve our understanding of the fundamental mechanism of bipolar pulse-induced PM-EP.Graphical AbstractHighlightsQuantitative and qualitative definition of the three states of the phospholipid membrane electroporation at the end of the intervals.Clarification that the states of phospholipid membrane electroporation were generated due to the combined effect of positive pulses and intervals.Quantitative and qualitative comparison the evolution of the states of phospholipid membrane electroporation during the negative pulses.
Impact of health warning labels and calorie labels on selection and purchasing of alcoholic and non-alcoholic drinks: a randomised controlled trial
Authors: Clarke Natasha , Ferrar Jennifer , Pechey Emily , Ventsel Minna , Pilling Mark A , Munafò Marcus R , Marteau Theresa M , Hollands Gareth J 
Published: July 2022

AbstractObjectiveHealth warning and calorie labels on alcohol have the potential to reduce consumption at population level but remain unevaluated using robust designs with behavioural outcomes. The aim of the current study is to estimate the impact on selection and actual purchasing of (a) health warning labels (text-only and image-and-text) on alcoholic drinks and (b) calorie labels on alcoholic and non-alcoholic drinks.DesignParallel-groups randomised controlled trial.SettingParticipants selected drinks in a simulated online supermarket, before purchasing them in an actual online supermarket.ParticipantsAdults in England and Wales who regularly consumed and purchased beer or wine online (n= 644).InterventionsParticipants were randomised to one of six groups in a between-subjects 3 (Health warning labels (HWLs): i. image-and-text HWL, ii. text-only HWL, iii. no HWL) x 2 (Calorie labels: present vs absent) factorial design.Main outcome measuresThe number of alcohol units selected (with intention to purchase); secondary outcomes included alcohol units purchased and calories selected and purchased.Results608 participants completed the study and were included in the primary analysis. There was no evidence of an overall difference for either (a) HWLs, or (b) calorie labels on the number of alcohol units selected [HWLs: F(2,599) = 0.406, p = .666; calorie labels: F(1,599) = 0.002, p = .961]. There was also no evidence of an overall difference on any secondary outcomes, including the number of alcohol units purchased [HWLs: F(2,462) = 1.85, p = .159; calorie labels: F(1,462) = 0.193, p = .661].In pre-specified subgroup analyses comparing the ‘calorie label only’ group (n = 101) to the ‘no label’ group (n = 104) there was no evidence that calorie labels reduced the number of calories selected [unadjusted means: 1913 calories vs 2203 calories, p = .643]. Amongst the 75% of participants who went on to purchase drinks, those in the ‘calorie label only’ group (n = 74) purchased fewer calories than those in the ‘no label’ group (n = 79) [unadjusted means: 1532 calories vs 2090, p = .028].ConclusionThere was no evidence that health warning labels reduced the number of alcohol units selected or purchased in an online purchasing setting. There was some evidence suggesting that calorie labels on alcoholic and non-alcoholic drinks may reduce calories purchased. Further evaluation is warranted in suitably powered studies in real world settings.Trial registrationPre-registered protocol (https://osf.io/ch2sm/) and prospective ISCRTN registration:https://www.isrctn.com/ISRCTN10313219FundingThis study was funded by Wellcome [Grant number 206853/Z/17/Z].
An unexpected histone chaperone function for the MIER1 histone deacetylase complex
Authors: Wang Siyu , Fairall Louise , Pham Khoa , Ragan Timothy J , Vashi Dipti , Collins Mark O. , Dominguez Cyril , Schwabe John W.R. 
Published: July 2022

AbstractHistone deacetylases 1 and 2 (HDAC1/2) serve as the catalytic subunit of six distinct families of nuclear complexes. These complexes repress gene transcription through removing acetyl groups from lysine residues in histone tails. In addition to the deacetylase subunit, these complexes typically contain transcription factor and/or chromatin binding activities. The MIER:HDAC complex has hitherto been poorly characterized. Here we show that MIER1 unexpectedly co-purifies with an H2A:H2B histone dimer. We show that MIER1 is also able to bind a complete histone octamer. Intriguingly, we found that a larger MIER1:HDAC1:BAHD1:C1QBP complex additionally co-purifies with an intact nucleosome on which H3K27 is either di- or tri-methylated. Together this suggests that the MIER1 complex acts downstream of PRC2 to expand regions of repressed chromatin and to deposit histone octamer onto nucleosome-depleted regions of DNA.
Sharīʿah scrutiny of Islamic Banks' Financial Compensation Fund in Bangladesh: governance principles in the COVID-19 perspective
Authors: Masum Abdullah , Islam S M Shariful 
Published: July 2022

PurposeThe purpose of this study is to critically analyze the Financial Compensation Funds being accumulated by Islamic Banks of Bangladesh in credit-based transactions. In this connection, due to the evolved liquidity crisis amidst the COVID-19, industry opinions are observed that suggest including the compensations or the donation funds directly into the bank's income account. But the Sharīʿah does not permit it. Such alternative proposals of using compensation or donation fund during crises are scrutinized under Sharīʿah principles to come to a logical conclusion.Design/methodology/approachThe approach followed in the study is textual and discourse analysis through descriptions of ideal Sharīʿah-compliant methods for handling late payment of credit and comparison with the industry practices.FindingsIt is observed that there are conceptual gaps in the industry as is reflected in the Islamic Banking Guideline of Bangladesh. The funds collected from the debtor due to late payment are named as compensation (Ta‘wīḍ) whereas the nature of the transaction is a donation (Tabarru'). The misconception can lead to various Sharīʿah non-compliant activities later with the funds. The proposals brought out in the industry to use such compensation/donation funds during a crisis are a consequence of this. The proposals of using such funds for banks' purposes in any situation are not supported by Sharīʿah principles and are against the Islamic banking philosophy.Originality/valueThe study is very relevant to the current crisis of COVID-19 in the domestic Islamic Banking Industry and also instrumental for the future guidance to stick to the Sharīʿah principles in managing compensation or donation funds by the Islamic Banks.
Impact of a physician's prosocial behavior on the patient's choice: an empirical investigation in online health community
Authors: Wang Jian-Jun , Liu Huiyuan , Cui Xiaocong , Ye Jiao , Chen Haozhe 
Published: July 2022

PurposeThe purpose of this paper is to explore the influence of a physician’s prosocial behavior on a patient's choices in the online health community (OHC) context. Moreover, the authors explore how such effects differ across different online word-of-mouth (WOM) and professional titles.Design/methodology/approachGuided by the motivation, opportunity and ability (MOA) framework, this paper develops hypotheses and an econometric model. Then this paper used spline regression to test hypotheses on 6,204 physicians at The Good Doctor (www.Haodf.com), which is one of the largest Chinese OHCs. The authors conducted the propensity score matching and difference-in-difference method (PSM-DID) to address the concern about the bias caused by possible endogeneity concerns.FindingsThe authors’ results show that a physician’s prosocial behavior improves a patient's choice only when the strength of a physician’s prosocial behavior is below the tipping point. In addition, the influence of a physician’s prosocial behavior is heterogeneous for physicians with different online WOM and professional titles. For physicians with higher online WOM, the effect of a physician's prosocial behaviors on a patient's choice is positive, while for physicians with lower online WOM, a physician’s prosocial behavior has no impact on a patient’s choice. For physicians with higher professional titles, the quantity of a physician’s prosocial behavior has a positive impact on a patient’s choice, while for physicians with lower professional titles the quality of a physician’s prosocial behavior has a positive impact on a patient’s choice.Originality/valueThis study contributes new knowledge and provides new perspectives to study a patient's choice by addressing the importance of physician's prosocial behavior. With the effort of explicitly explaining the complex mechanisms, this study encourages physicians' engagement in a physician’s prosocial behavior and gives some implications on how to perform the behaviors strategically.
Industry 4.0: what is the relationship between manufacturing strategies, critical success factors and technology adoption?
Authors: Tortorella Guilherme , Kurnia Sherah , Trentin Marcelo , Oliveira Gilson Adamczuk , Setti Dalmarino 
Published: July 2022

PurposeThis paper examines the relationship between different manufacturing strategies and Industry 4.0's (I4.0) critical success factors (CSFs) and technology adoption.Design/methodology/approachFor that, the authors surveyed 165 practitioners from different manufacturers. Participants provided information about the levels of product customization and production volume in their companies. They also indicated the adoption level of I4.0 technologies and CSFs. Using multivariate data techniques, the authors identified four clusters of different manufacturing strategies and two readiness levels based on the establishment of I4.0 CSFs. The adoption level of I4.0 technologies was then cross compared among clusters to identify which technologies are more likely to be supported.FindingsThe findings indicate that, in low-readiness companies, the adoption level of I4.0 technologies does not significantly differ between manufacturing strategies. However, when companies present a higher I4.0 readiness, the adoption of I4.0 technologies seem to vary according to the existing manufacturing strategy.Originality/valueThis study sheds light on the influence that manufacturing strategies may have on the digital transformation of companies, highlighting which strategies are more likely to offer a context to successfully adopt I4.0 technologies. The identification of these relationships helps to define the expectation regarding the company's digital transformation, determining coherent benchmarks and allowing managers to anticipate potential issues.
The lived experience of Australian public library staff during the COVID-19 library closures
Authors: Garner Jane , Wakeling Simon , Hider Philip , Jamali Hamid R. , Kennan Mary Anne , Mansourian Yazdan , Randell-Moon Holly 
Published: July 2022

PurposeThe purpose of this paper is to explore the lived experiences of Australian public library staff during the COVID-19 library closures. The study examines the effect of mandated physical library closures on staff well-being, along with the challenges they faced as library operations moved to a remotely delivered model. The paper includes an examination of staff perceptions of their library's value in the lives of their users.Design/methodology/approachSemi-structured interviews were undertaken with 15 Australian library staff from three library networks. A process of inductive coding resulted in a thematic description of the participants' experiences of continuing to work during a period of where their libraries were closed due to COVID-19 restrictions.FindingsAustralian public library workers experienced many challenges that affected their well-being during the period of library closures. These included challenges relating to moving library programming to a virtual delivery model, managing significant change in their work lives, managing the emotions of self and others, and concern for the well-being of library users. Positive outcomes relating to skill development and innovative thinking were also reported.Originality/valueThe operational responses to the COVID-19 library closures in Australia and elsewhere have been well reported. This paper takes a different approach by examining the emotional and well-being outcomes for public library staff during these periods of closure.
Development and characterization of nitrocellulose/acrylic-based coatings with precipitated silica and kaolin for packaging applications
Authors: Kavas Akarca Tuba , Karayol Merve , Yuzay Isinay E. 
Published: July 2022

Purpose
The purpose of this study is to develop a multifunctional coating layer based on nitrocellulose (NC)/acrylic resins containing precipitated silica and kaolin and investigate its suitability for use in packaging applications.

Design/methodology/approach
Different loading levels (1 and 5 Wt.%) of precipitated silica or kaolin particles were incorporated into NC/acrylic-based coating formulations and applied on low-density polyethylene (LDPE) films. The coatings and coated LDPE films were characterized in terms of structural, physical, mechanical, thermal, optical, surface, morphological and water vapor barrier properties.

Findings
The glossiness of the coating formulations decreased by increasing the precipitated silica and kaolin content. The incorporation of kaolin (1 and 5 Wt.%) and precipitated silica (1 Wt.%) had no significant effect on the melting temperature of LDPE film; however, with the addition of 5 Wt.% precipitated silica, the melting and crystallization temperatures were significantly changed. The incorporation of 5 Wt.% precipitated silica and kaolin also enhanced the water vapor barrier properties of LDPE films. The light transmittance declined with the precipitated silica and kaolin addition, especially in the ultraviolet (UV)-A/UV-B spectrum regions indicating an excellent UV light protection.

Originality/value
It was concluded that NC/acrylic resins coatings containing precipitated silica and kaolin exhibit improved thermal stability, UV and water vapor barrier properties and have the potential for use in packaging applications.
Inclusivity as civism: theorizing the axiology of marketing and branding of places
Authors: Lucarelli Andrea 
Published: July 2022

Purpose
This study aims to outline an axiology of inclusivity, which can facilitate self-reflection on the possible impact of acting and pursuing a more inclusive branding and marketing for places.

Design/methodology/approach
By deconstructing the main assumption, which constitutes the new inclusive paradigm in the marketing and branding of places as more participatory, responsible and democratic, this article tackles critical and pragmatist concerns about the political dimension and its implications for branding and marketing theories and practices in the realm of places.

Findings
The article argues that, to be understood and enacted as inclusive, branding and marketing should be seen and act as (bio)political arts of government, characterized by the impolitical as an alternative form of political praxis, whose axiological foundation is based on a particular form of civism, which offers a different mode and stance of approaching political effects and impacts for all stakeholders involved.

Originality/value
Little has been written about the political value, substance and appearance that indicate inclusivity as a fundamental notion for participation, engagement and democracy. This article contributes to the existing literature, arguing that inclusivity should be demystified, as it may present a self-fulfilling discourse that might create political problems.