bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of boron on fat synthesis in porcine mammary epithelial cells
Authors: Yang Yanan , Ren Man 
Published: December 2022

Abstract
Abstract: This study aimed to investigate the effect of boron on porcine mammary epithelial cell (PMEC) survival, cell cycle and milk fat synthesis. PMECs from boron-treated groups were exposed to 0, 0.1, 0.2, 0.3, 0.4, 0.8, 1, 10, 20, 40, and 80 mmol/L boric acid concentrations. Cell Counting Kit-8 and flow cytometry assays were performed to assess cell survival and the cell cycle, respectively. Triacylglycerol (TAG) levels in PMECs and culture medium were determined by a Triacylglycerol kit while PMEC lipid droplet aggregation was investigated via Oil Red Staining. Milk fat synthesis-associated mRNA levels were determined by real-time fluorescence quantitative PCR while its protein expressions were determined by Western blot. Low (0.2, 0.3, 0.4 mmol/L) and high (>10 mmol/L) boron concentrations significantly promoted and inhibited cell viabilities, respectively. Boron (0.3 mmol/L) markedly elevated the abundance of G2/M phase cells. Ten mmol/L boron significantly increased the abundances of G0/G1 and S phase cells, but markedly suppressed G2/M phase cell abundance. At 0.3 mmol/L, boron significantly enhanced ERK phosphorylation while at 0.4, 0.8, 1, and 10 mmol/L, it markedly decreased lipid droplet diameters. Boron (10 mmol/L) significantly suppressed ACACA and SREBP1 protein expressions. The FASN protein levels were markedly suppressed by 0.4, 0.8, 1 and 10 mmol/L boron. Both 1 and 10 mmol/L markedly decreased FASN and SREBP1 mRNA expressions. Ten mmol/L boron significantly decreased PPARα mRNA levels. Low concentrations of boron promoted cell viability, while high concentrations inhibited PMEC viabilities and reduced lipid droplet diameters, which shows the implications of boron in pregnancy and lactation.
Particulate Matter 10 -induced airway inflammation and fibrosis can be regulated by chitinase-1 suppression
Authors: Choi Yong Jun , Han Heejae , Lee Jaeuk , Kim Chi Young , Byun Min Kwang , Cho Jae Hwa , Park Hye Jung 
Published: December 2022

Abstract
Background
Particulate matter10 (PM10) can induce airway inflammation and fibrosis. Chitinase-1 is recently known to have key roles in inflammation and fibrosis. We aimed to investigate the effects of chitinase-1 inhibitor in PM10-treated murine models.
Methods
In female BALB/c mice, PM10 was intranasally administered four times over 2 weeks, and ovalbumin (OVA) was intraperitoneally injected and then intranasally administered. Chitinase-1 inhibitor (CPX) 6 times over 3 weeks or dexamethasone 3 times in last week were treated intraperitoneally. After 2 days from the last challenges, mice were sacrificed. Full RNA sequencing using lung homogenates was conducted to evaluate signaling pathway.
Results
PM10 and/or OVA-induced airway inflammation and fibrosis murine models were well established. CPX and dexamethasone ameliorated PM10 or PM10/OVA-induced airway hyper-responsiveness, airway inflammation, and fibrosis. CPX and dexamethasone also reduced levels of various inflammatory markers including chitinase-1 in lung homogenates. PM10 and OVA also induced extreme changes of mRNA expression. CPX and dexamethasone decreased levels of mRNA expression especially associated with inflammation and immune regulation. They also significantly regulated asthma and asthma related pathway including JACK-STAT signaling pathway.
Conclusions
Chitinase-1 suppression by CPX can regulate PM10-induced and aggravated airway inflammation and fibrosis via various signaling pathway.
Feasibility Study on Mega-Watt level Fast charging system for shore power supply using wireless power transfer technology
Authors: Aditya Kunwar , Pradhan Sushan , Munsi Avishek 
Published: December 2022

Abstract
This paper investigates the challenges associated with building MW level wireless chargers using inductive power transfer (IPT) technology for marine energy storage systems. Currently, the highest power capacity for the IPT system is 1 MW by Korea RailRoad Institute for high in-motion charging of speed trains. However, for stationary charging applications, maximum power capacity implemented using IPT is only up to 200 kW accomplished by Bombardier PRIMOVE for bus charging. For marine application, charging can take place at quay, therefore only static charging is possible. In this paper, the specifications of MF Ampere ferry have been used for the feasibility study. Through analytical calculations and PSIM Simulation, it was established that several low powers (100 kW IPT systems) need to be paralleled for 1 MW power transfer. Building a single unit of 1 MW is not feasible due to involved voltage and current stress across the components and limited space available onboard as well as onshore. 100 kW power level can be implemented despite of involved misalignments. However, if primary is somehow kept stationary with respect to secondary, for example using vacuum pad mooring technology developed by Wärtsilä and Cavotec, then a single unit of 200 kW could be implemented without any forced cooling. 10 units of 100 kW (without any mechanical mooring assistance) or 5 units of 200 kW (with mechanical mooring assistance) are the two proposed solutions for implementing a fast charger for 1 MW level.
Targeting homologous recombination repair in BCR/ABL1-positive cells using PARP inhibitor
Authors: Hiroki Haruka , Ishii Yuko , Piao Jinhua , Namikawa Yui , Masutani Mitsuko , Honda Hiroaki , Akahane Koushi , Inukai Takeshi , Morio Tomohiro , Takagi Masatoshi 
Published: December 2022

Abstract
BCR/ABL1 causes dysregulated cell proliferation and is responsible for chronic myelogenous leukemia (CML) and Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph1-ALL). In addition to the deregulatory effects of its kinase activity on cell proliferation, BCR/ABL1 induces genomic instability by downregulating BRCA1. PARP inhibitors (PARPi) effectively induce cell death in BRCA-defective cells. Therefore, PARPi are expected to inhibit the growth of CML and Ph1-ALL cells with downregulated BRCA1 expression. Inhibition of BCR/ABL1-mediated leukemogenesis by PARP inhibition was tested in two in vivo models: wild-type mice that had undergone hematopoietic cell transplantation with BCR/ABL1-transduced cells, and a genetic model constructed by crossing Parp1 knockout mice with BCR/ABL1 transgenic mice. Here, we demonstrate that a PARPi, olaparib, attenuates BCR/ABL1-mediated leukemogenesis. Inhibition of leukemogenesis in BCR/ABL1-positive cells was the result of a combination of the direct cytotoxic effects of olaparib and reduced maintenance of BCR/ABL1-positive stem cells. Interferon was used as a first-line therapy for CML.Activation of the cGAS/STING pathway was also observed upon PARP inhibition. Because tyrosine kinase inhibitor (TKI) monotherapy does not completely eradicate leukemic cells in all patients, PARPi could be combined with TKIs to induce CML cell death more effectively.
Novel strategies for the preparation of nano-silver pectin sponge composite and its antibacterial properties
Authors: Zhao Zhi-yuan , Li Pei-jun , Cao Xing-ye , Xie Run-sheng , Li Hai-yun 
Published: December 2022

Abstract
Biodegradable sponges that can be used in the medical field are currently attracting a lot of attention, but the development of safe materials with high antimicrobial activity is still a major challenge. Doping with silver nanoparticles (AgNPs) can effectively solve this problem. In this study, pectin sponges (PE-S) and nano-silver pectin sponges (PE-S/Ag) were synthesized in situ by freeze-drying method. The prepared sponge samples were characterized by various tools. UV-vis spectroscopy showed the in situ synthesis of AgNPs; FT-IR spectroscopy and XPS investigated the functional group changes in the sponge samples; SEM images confirmed the homogeneous distribution of AgNPs in the PE-S/Ag sponge. The physical and chemical properties of PE-S/Ag sponges were higher than PE-S sponges. The water absorption of PE-S/Ag and PE-S sponges was 2602.04% and 1562.20% respectively; the porosity was 83.36% and 66.61%, respectively. The results of the antibacterial test showed that the minimum inhibitory concentration (MIC) of PE-S/Ag sponge was 42 and 83 µg/mL for Escherichia coli and Staphylococcus aureus, respectively. The results of the cytotoxicity and coagulation assays showed that the two sponge samples prepared were non-toxic to cells and had a good coagulation effect. Silver ion (Ag+) release studies showed the sustainability of Ag+ release from PE-S/Ag sponges. These preliminary data showed that PE-S/Ag sponges have excellent performance to meet the antimicrobial needs of a wide range of medical applications.
Corpus callosum organization and its implication to core and comorbid symptoms of Autism Spectrum Disorder
Authors: Minnigulova Alina , Davydova Elizaveta , Pereverzeva Darya , Sorokin Alexander , Tyushkevich Svetlana , Mamokhina Uliana , Danilina Kamilla , Dragoy Olga , Arutiunian Vardan 
Published: December 2022

Abstract
Background: Autism Spectrum Disorder (ASD) is characterized by social interaction and communication deficits, repetitive behavior and often by co-occurring conditions such as language and non-verbal IQ development delays. Previous studies reported that those behavioral abnormalities can be associated with corpus callosum organization. However, little is known about the specific differences in white matter structure of the corpus callosum parts in children with ASD and TD peers and their relationships to core and comorbid symptoms of ASD. The aim of the study was to investigate the volumetric and microstructural characteristics of the corpus callosum parts crucially involved in social, language, and non-verbal IQ behavior in primary-school-aged children with ASD and to assess the relationships between these characteristics and behavioral measures.
Method: 38 children (19 with ASD, 19 typically developing (TD)) were scanned using diffusion-weighted MRI and behaviorally assessed. The tractography of the corpus callosum parts were performed using Quantitative Imaging Toolkit software; diffusivity and volumetric measurements were extracted and analyzed.
Results: In the ASD group, fractional anisotropy (FA) was decreased across the supplementary motor area and the ventromedial prefrontal cortex, axial diffusivity (AD) was reduced across each of the corpus callosum parts in comparison to the TD group. Importantly, the AD decrease was related to worse language abilities and more severe autistic traits in individuals with ASD.
Conclusions: The microstructure of the corpus callosum parts differs between children with and without ASD. Abnormalities in white matter organization of the corpus callosum parts are associated with core and comorbid symptoms of ASD.
Cardiovascular Toxicities after Anthracycline and VEGF-Targeted Therapies in Adolescent and Young Adult Cancer Survivors
Authors: Wong-Siegel Jeannette R. , Hayashi Robert J. , Foraker Randi , Mitchell Joshua D. 
Published: December 2022

Abstract
Background
Cancer survival rates have been steadily improving in the adolescent and young adult (AYA) population, but survivors are at increased risk for cardiovascular disease (CVD). The cardiotoxic effects of anthracycline therapy have been well studied. However, the cardiovascular toxicity associated with newer therapies, such as the vascular endothelial growth factor (VEGF) inhibitors, is less well understood.
Objective
This retrospective study of AYA cancer survivors sought to gain insight into their burden of cardiovascular toxicities (CT) following anthracycline and/or VEGF inhibitor therapy.
Methods
Data were extracted from electronic medical records over a fourteen-year period at a single institution. We utilized Cox proportional hazards regression modeling to examine risk factors for CT within each treatment group. Cumulative incidence was calculated with death as a competing risk.
Results
Of the 1,165 AYA cancer survivors examined, 32%, 22%, and 34% of patients treated with anthracycline, VEGF inhibitor, or both, developed CT. Hypertension was the most common outcome reported. Males were at increased risk for CT following anthracycline therapy (HR: 1.34, 95% CI 1.04–1.73). The cumulative incidence of CT was highest in patients who received both anthracycline and VEGF inhibitor (50% at ten years of follow up).
Conclusions
CT was common among AYA cancer survivors who received anthracycline and/or VEGF inhibitor therapy. Male sex was an independent risk factor for CT following anthracycline treatment. Further screening and surveillance are warranted to continue understanding the burden of CVD following VEGF inhibitor therapy.
Differences in perceived threat and trauma in children during the Covid-19 pandemic
Authors: Kohlboeck Gabriele , Wenter Anna , Sevecke Kathrin , Exenberger Silvia 
Published: December 2022

Abstract
Background
The past two years of the COVID-19 pandemic were stressful to most children and adolescents, and some of the children may have experienced a high level of stress and trauma. To date, no study has examined differences in self-reported stress and trauma levels due to COVID-19 in children. This study aimed to assess the differences between perceived threat, exposure and trauma symptoms in children aged 7–13 years. In addition, we explored whether parent-reported factors could predict a higher risk of COVID-19 vulnerability in their children.
Method
Cross-sectional data from 752 children were assessed with regard to COVID-19 threat and exposure and trauma symptoms using the self- and parent-reported CATS Trauma questionnaire. We used exploratory data analysis methods (factor analysis of mixed data and hierarchical clustering) to identify subgroups (i.e. clusters) of children sharing similar characteristics in the data set. Linear regression modelling was applied to determine the likelihood of higher threat and vulnerability in children with parent-reported COVID-19 threat, exposure, CATS trauma symptoms and behaviour using the child behaviour checklist (CBCL).
Results
We identified a high-risk group of children reporting clinically relevant trauma symptoms and COVID-19 related fears. Parents’ reports of trauma could identify these.
Conclusions
In about 25% of children clinical relevant trauma symptoms and threat are present. It is especially important to offer adequate support to ease the trauma and prevent development into further psychopathology.
Effects of paraprobiotic as replacements for antibiotic on performance, immunity, gut health and carcass characteristics in broiler chickens
Authors: Mukesh N. T , Biswas Avishek , Deo Chandra , Jayanti L. A. , Monika M. , Tiwari Ashok Kumar 
Published: December 2022

Abstract
This study sought to determine the effects of dietary paraprobiotic (PPB) on broiler chicken performance, immunity, gut health, and carcass traits. 240 day-old CARIBRO Vishal commercial broiler chicks of identical body weight randomly divided into six treatment groups, each with five replicates and eight chicks in each replicate. Six dietary treatments were preapared: T1 = (control diet), T2 = T1 + 0.02 % (w/v) chlortetracycline (CTC), T3 = T1 + 0.2% (w/v) PPB, T4 = T1 + 0.4% (w/v) PPB, T5 = T1 + 0.6 % (w/v) PPB and T6 = T1 + 0.8 % (w/v) PPB, respectively. Body weight gain (BWG) significantly (P≤0.05) increased in the T5 (0.6 % PPB) and T6 (0.8% PPB) group. At the same time the feed intake significantly (P≤0.05) decreased and the feed conversion ratio (FCR) significantly (P≤0.05) improved in T5 and T6 group. There was a significant (P≤0.05) increase in cell-mediated immunity and haem-agglutination titre (HA titre) in the 0.6% and 0.8% PPB supplemented groups compare to the control group (T1). The percentage of carcass traits and organ weights did not significantly differ between the PPB-supplemented and control groups, but the percentage of live weight in cut up parts showed a significant improvement (P≤0.05) in the PPB-supplemented group. At 42 days, villus height, width, and crypt depth all significantly (P0.05) increased in the groups supplemented with 0.6 and 0.8% para-probiotics (T5 and T6). The results show that para-probiotics can be added to broiler diets at a rate of 0.6% (w/v) to enhance performance, immunity, gut health, and breast yield. The para-probiotic may therefore be a useful substitution for antibiotic growth promoters in the diet of chickens.
Sobolev’s type optimal topology in the problem of exact observability for Hilbert space dynamical systems connected with Riesz basis of divided differences
Authors: Woźniak Jarosław , Firkowski Mateusz 
Published: December 2022

Abstract
This paper considers the problem of exact observability of a general class of distributed parameter systems in Hilbert spaces, connected to Riesz basis properties of some families of exponential functions and the divided differences of those functions. Under some assumptions on asymptotic spectral analysis of the differential operator of the system, the conditions of exact observability are stated in the form of exact observable spaces being the direct sum of some specific Sobolev spaces. The main result consists of proving the optimality of these subspaces of observable states. The result was based on advanced non-harmonic analysis approach connected to the unusual fact that time-space Riesz basis does not consist only of exponential functions but also contains divided differences of these functions.
Concerned friends of intimate partner violence survivors: Results from the myPlan randomized controlled trial on college campuses
Authors: Bloom Tina L. , Perrin Nancy , Brown Megan Lindsay , Campbell Jacquelyn , Clough Amber , Grace Karen Trister , Laughon Kathryn , Messing Jill , Eden Karen B. , Turner Rachael , Glass Nancy 
Published: December 2022

Abstract
Background: Nearly half of intimate partner violence (IPV) survivors experience their first abusive relationship at college age (18-24 years), and most often these young people disclose the violence to friends. Existing "bystander" interventions targeting college campuses focus on training peers to safely intervene and have been effective in sexual assault prevention; similar interventions have rarely been tested for IPV intervention. Therefore, we evaluated the effectiveness of an interactive, personalized safety decision and planning tool, myPlan app, on decisional conflict, attitudes toward IPV, confidence in intervening, and supportive safety behaviors with concerned friends of abused college women.
Methods: We recruited college students (age 18-24, N=293) of any gender who indicated they had a female-identified friend who had recently experienced IPV (“concerned friends”) from 41 Oregon and Maryland colleges/universities. Participants were randomized to myPlan (n=147) or control (usual web-based resources; n=146) and outcomes (e.g., decisional conflict, confidence to intervene, safety/support behaviors, attitudes toward IPV) were measured at baseline and 12-months later.
Results: Concerned friends described the person being abused as a close/best friend (79.1%). Most (89.2%) reported concern that their friend would be seriously hurt by her abusive partner, with 22.7% reporting extreme concern. Intervention participants had a greater reduction in decisional conflict related to clarity in their own values immediately after their first use of myPlan. At 12-month follow-up, groups did not differ significantly in terms of percentage of safety/support strategies used, whether strategies were helpful, or attitudes toward IPV. Both groups reported increased confidence to intervene, but the intervention group had a significantly greater increase in confidence to talk with someone about concerns in their own relationships than the control group.
Conclusions: A technology-based intervention, myPlan, was effective in reducing decisional conflict and clarifying values about supporting a friend in an unsafe relationship. Information on IPV and related safety strategies delivered through the myPlan app or usual web-based resources both increased confidence to intervene with a friend. College students in the myPlan group were more likely to talk with someone about concerns about their own relationship, demonstrating the potential of myPlan as a tool for IPV prevention or early intervention. Trial Registration: Clinicaltrials.gov ID: NCT02236663
Impact of Social Capital on Chinese Migrant Workers' Poverty during COVID-19: The Mediation of Social Protection
Authors: Chen Chen , Gan Christopher 
Published: December 2022

Abstract
Attention to and investigation of migrant workers’ poverty level in the COVID-19 environment are vital for understanding their living conditions. It is also critical to explore the effect of social capital and protection on migrant workers’ poverty alleviation in the post-COVID era. Using an online survey, this study examines the relationship between migrant workers’ social capital and poverty alleviation at the aggregate and dimensional levels from the multidimensional poverty perspective in the COVID-19 environment. We find that, in the COVID-19 environment, migrant workers’ social capital in the cities is notable for mitigating their poverty, and accessible social protection can weaken this relationship at the aggregate level. At the dimensional level, the result shows that migrant workers’ poverty alleviation depends on their social network and reciprocal connections in the cities, not social trust. However, migrant workers’ accessible social protections can mediate the relationship between the three dimensions of social capital and poverty. Our findings provide new evidence for the good and dark sides of the social capital and poverty alleviation relationship. Our result also shows that social protection can mitigate the influence of unequal social capital on poverty to achieve a more balanced result. The findings suggest that governments should design more inclusive but targeted social protection policies for migrant workers to decrease the effect of unequal social capital and so increase the impact on poverty alleviation.
JEL classification: D10; I32; J61
Insecticidal plant oil’s toxicity activities on the larval and adult stages of a major malaria vector (Anopheles gambiae Giles 1920)
Authors: Adelaja Olukayode James , Oduola Adedayo Olatubosun , Ande Adeolu Taiwo , Abiodun Oyindamola Olajumoke , Adelaja Abisayo Ruth 
Published: December 2022

Abstract
Despite increasing reports and concerns about resistance development to public-health insecticides in malaria vectors, significant steps have been put into the quest for novel strategies to disrupt the disease transmission cycle by targeting insect vectors hence sustaining vector management. This study evaluates the toxicity potential of oils of insecticidal plants shortlisted in an ethnobotanical survey on the larvae and adult stages of Anopheles gambiae. Oils from leaves of Hyptis suaveolens, Ocimum gratissimum, Nicotiana tabacum, Ageratum conyzoides and fruit-peel of Citrus sinensis were extracted by steam-distillation using a Clevenger apparatus. Larvae and female adults of deltamethrin-susceptible Anopheles gambiae were gotten from an already established colony in the Entomological Research Laboratory, University of Ilorin. Twenty-five third instar stage larvae were used for larvicidal assays while twenty 2-5 days old adults were used for the adulticidal assays in five replicates. A. gambiae exposed to H. suaveolens and C. sinensis demonstrated significantly higher larval toxicity (94.7-100%) after 24 hours. At 48 hours, the mortality induced by the oils of the four plants peaked at 100%. N. tabacum (0.50 mg/ml) induced the highest percentage of adult mortality (100%) on A. gambiae which was compared favourably with the positive control Deltamethrin (0.05%). The lowest KdT50 was observed with 0.25 mg/ml of N. tabacum (20.3 minutes) while the lowest KdT95 was observed with 0.10mg/ml of A. conyzoides (35.97 mins) against adult A. gambiae. The significant larval and adult mortality rates, lower lethal concentration and knockdown times demonstrated by the evaluated plant oils showed promising outcomes that can be further developed for vector control management.