bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Effects of Exosomes and Ectosomes Isolated From Adipose-Derived Mesenchymal Stem Cells on Achilles Tendinopathy in a Rat Model
Authors: Xu Tengjing , Lin Yunting , Yu Xinning , Jiang Guangyao , Wang Jiajie , Xu Kaiwang , Fang Jinghua , Wang Siheng , Dai Xuesong 
Published: July 2022

Background: Extracellular vesicles derived from mesenchymal stem cells (MSC-EVs) have gained momentum as a treatment for tendinopathy. Multiple studies have demonstrated significant differences in cargo composition between the 2 subtypes of MSC-EVs (ie, exosomes and ectosomes), which may result in different therapeutic effects. However, the effects of the 2 EV subtypes on tendinopathy have not yet been compared. Purpose: To compare the effects of adipose stem cell–derived exosomes (ASC-Exos) and ectosomes (ASC-Ectos) on Achilles tendinopathy. Study Design: Controlled laboratory study. Methods: Rats were administered collagenase injections to generate a model of Achilles tendinopathy. A week later, 36 rats were randomly assigned to 3 groups. In each group, Achilles tendons were injected with equal volumes of ASC-Exos, ASC-Ectos, or saline (12 legs/group). The healing outcomes were evaluated by magnetic resonance imaging, histology, immunohistochemistry, transmission electron microscopy, and biomechanical testing at 3 and 5 weeks after collagenase injection. Results: At 3 and 5 weeks, the ASC-Exo group had better histological scores ( P = .0036 and P = .0276, respectively), a lower fibril density ( P < .0001 and P = .0310, respectively), and a larger collagen diameter ( P = .0052 and P < .0001, respectively) than the ASC-Ecto group. At 5 weeks, the expression of collagen type 1 and CD206 in the ASC-Exo group was significantly higher than that in the ASC-Ecto group ( P = .0025 and P = .0010, respectively). Regarding biomechanical testing, the ASC-Exo group showed higher failure load ( P = .0005), tensile stress ( P < .0001), and elastic modulus ( P < .0001) than the ASC-Ecto group. Conclusion: ASC-Exos had more beneficial effects on tendon repair than ASC-Ectos in a rat model of Achilles tendinopathy. Clinical Relevance: Administration of ASC-EVs may have the potential to treat Achilles tendinopathy, and delivery of ASC-Exos could provide additional benefits. It is necessary to compare the healing responses caused by different EV subtypes to further understand their effects on tendinopathy and to aid clinical decision making.
Comparison of Acetabular Measurements Between 2 Validated Software Programs Used in Hip Preservation Surgery
Authors: Laboudie Pierre , Fischman Daniel , Speirs Andrew D. , Salih Saif , Holc Fernando , Beaule Paul E. , Witt Johan D. , Grammatopoulos George 
Published: July 2022

Background: Validated software tools (Clinical Graphics [CG] and Hip2Norm) permit measurement of the percentage of femoral head coverage (%FHC), which aids in morphological classification and prediction of outcome after hip preservation surgery. Purpose: (1) To assess whether acetabular parameter measurements determined from 2 commonly used software systems are comparable. (2) To determine which parameters influence the correlation or differences between software outputs and measurements. Study Design: Cohort study (diagnosis); Level of evidence, 2. Methods: The study included 69 patients (90 hips) who underwent periacetabular osteotomy and had comprehensive preoperative imaging available. Lateral center-edge angle (LCEA), acetabular index (AI), and %FHC were determined using 3-dimensional computed tomography (CT) measurements by CG and Hip2Norm software. Images of 18 pelvises were segmented to determine spinopelvic parameters and subtended acetabular angles. Between-group measurements were compared using correlation coefficients and Bland-Altman analyses. The difference in the outputs of the 2 programs was defined as delta (Δ). Radiographic parameters were tested to assess whether they were responsible for differences in %FHC between software programs. Results: Strong correlations between LCEA (ρ = 0.862) and AI (ρ = 0.825) measurements were seen between the Hip2Norm and CG programs. However, weak correlation was seen in the estimate of %FHC (ρ = 0.358), with the presence of a systematic error. Hip2Norm consistently produced lower anterior, posterior, and total %FHC values than CG. The %FHC determined by CG, but not Hip2Norm, correlated with acetabular subtended angles ( P < .05). Pelvic tilt measured on CT did not correlate with pelvic tilt estimated by Hip2Norm ( P = .56), and ΔPelvicTilt strongly correlated with the difference in %FHC by the 2 software programs (ρ = 0.63; P = .005), pelvic incidence (ρ = 0.73; P < .001), and pelvic tilt (ρ = −0.91; P < .001) as per CT. Conclusion: The correlation of %FHC between Hip2Norm and CG was weak (ρ = 0.358). The difference in measurements of %FHC correlated with ΔPelvicTilt. The %FHC determined by CG strongly correlated with the segmented acetabular subtended angles and thus more likely reflected true values. Hip preservation surgeons should be aware of these measurement differences because %FHC is important in the diagnosis and prognosis of acetabular dysplasia.
Biomechanical performance of the cemented acetabular cup with combined effects of bone quality, implant material combinations and bodyweight
Authors: Kumar Ajay , Mondal Subrata , Ghosh Rajesh 
Published: July 2022

The objective of this study is to understand the combined effects of bone quality, implant materials and bodyweight on the biomechanical performance of cemented acetabular cup. Additionally, the performance of the cemented acetabular cup was evaluated for obesity cases or obese people. A total of 84 FE models (based on CT data) were developed based on combinations of three different cancellous bone material distributions to represent bone quality, four different implant material combinations and seven different bodyweights. The biomechanical performance of the acetabular cup was evaluated based on bone stress (both cortical and cancellous bone), cement mantle stress, micromotion and contact pressure between the acetabular cup and femoral head. Cortical bone stress, cancellous bone stress, cement stress, the contact pressure between implants and micromotion between implants are affected by different bone quality, implant material combinations and bodyweights. An increase in bodyweight would increase the cortical bone stress, cancellous bone stress, cement stress, contact pressure between implants and micromotion between implants. However, bodyweight affects the cortical and cancellous bone stress more (stiff rise of the bone stresses; nonlinear relation) as compared to other output parameters (mostly linear relation). Comparing cortical and cancellous bone stress, the stress versus bodyweight curve is much stiffer (stiff rise in the curve) for cortical bone than cancellous bone and that even further increases as bone quality decreases. Especially considering obesity cases or obese people (very high bodyweight), the performance of the cemented acetabular component is poor. Graphical abstract [Formula: see text]
Women in Cantonments: Evolution of Regulated Military Prostitution in Colonial India
Authors: Mondal Sonia 
Published: July 2022

From the mid-19th century the existence of a large number of native prostitutes in the British military cantonments confirmed the widespread prevalence of state-authorised prostitution in colonial India. The colonial administration induced new laws that tried to restrict prostitution as a practice exclusively for the service of the British military men and also made it a sanctioned part of the military establishment. Consequently, a new form of regulated military prostitution evolved that radically changed the nature of prostitution as it used to be in the pre-colonial period. In this article, I have tried to explore the causes behind the emergence of regulated military prostitution and how it was implemented and systematised in British India. It would also focus on a new pattern of brothel life which emerged throughout military cantonments and finally the women’s life in cantonments that would provide a glimpse of their daily chores and challenges.
Comparison of Lapse Rate in Drug Dependent Patients in 2 Methods of Methadone Maintenance Treatment and Buprenorphine Maintenance Treatment
Authors: Vafaeinasab Mohammadreza , Zare Hamidreza , Dehghani Ali , Malek Seyedehzahra , Dehghani-Tafti Maryam , Sarebanhassanabadi Mohammadtaghi 
Published: July 2022

Background: Lapse has been one of the major challenges in the treatment of drug dependence sometimes leading to its relapse. Objectives: The aim of this study was to determine the lapse rate in drug dependent patients as for the 2 methods of methadone maintenance treatment (MMT) and buprenorphine maintenance treatment (BMT) in Yazd city. Methods: In this cross-sectional study, 626 female and male patients who had referred to 5 SUD treatment centers in Yazd and had been treated with methadone and buprenorphine maintenance were studied. Participants were divided into 2 groups of MMT and BMT and were evaluated based on lapse within 6 months. Results: In this study, 60.9% of patients were treated with methadone but the rest were treated with buprenorphine. Overall, 33.1% of patients lapsed (35.2% for methadone and 29.8%for buprenorphine). Lapse in methadone treatment was correlated with age, occupational status, and duration of treatment ( P < .05); it failed to correlated with any other demographic and clinical characteristics ( P > .05). Lapse rate in buprenorphine treatment was also related to marital status and the drug used ( P < .05). The mean dose of buprenorphine consumed showed no significant relationship with lapse ( P > .05). The results demonstrated that given the low dose, lapse stood higher in the buprenorphine group than the methadone group; however, as to high dose, the buprenorphine group showed lower lapse than the other group. Conclusions: In regard with the high rate of lapse, it is recommended to consider the factors related to the 2 methods of treatments, and provide counseling and training programs to lower lapse in the patients. Ethics Committee (REC) approval code: IR.SSU.REC.1394.158.
A platform trial design for preventive vaccines against Marburg virus and other emerging infectious disease threats
Authors: Longini Ira M , Yang Yang , Fleming Thomas R , Muñoz-Fontela César , Wang Rui , Ellenberg Susan S , Qian George , Halloran M Elizabeth , Nason Martha , Gruttola Victor De , Mulangu Sabue , Huang Yunda , Donnelly Christl A , Henao Restrepo Ana-Maria 
Published: July 2022

Background: The threat of a possible Marburg virus disease outbreak in Central and Western Africa is growing. While no Marburg virus vaccines are currently available for use, several candidates are in the pipeline. Building on knowledge and experiences in the designs of vaccine efficacy trials against other pathogens, including SARS-CoV-2, we develop designs of randomized Phase 3 vaccine efficacy trials for Marburg virus vaccines. Methods: A core protocol approach will be used, allowing multiple vaccine candidates to be tested against controls. The primary objective of the trial will be to evaluate the effect of each vaccine on the rate of virologically confirmed Marburg virus disease, although Marburg infection assessed via seroconversion could be the primary objective in some cases. The overall trial design will be a mixture of individually and cluster-randomized designs, with individual randomization done whenever possible. Clusters will consist of either contacts and contacts of contacts of index cases, that is, ring vaccination, or other transmission units. Results: The primary efficacy endpoint will be analysed as a time-to-event outcome. A vaccine will be considered successful if its estimated efficacy is greater than 50% and has sufficient precision to rule out that true efficacy is less than 30%. This will require approximately 150 total endpoints, that is, cases of confirmed Marburg virus disease, per vaccine/comparator combination. Interim analyses will be conducted after 50 and after 100 events. Statistical analysis of the trial will be blended across the different types of designs. Under the assumption of a 6-month attack rate of 1% of the participants in the placebo arm for both the individually and cluster-randomized populations, the most likely sample size is about 20,000 participants per arm. Conclusion: This event-driven design takes into the account the potentially sporadic spread of Marburg virus. The proposed trial design may be applicable for other pathogens against which effective vaccines are not yet available.
Exploring the concept of deep remission in Crohn’s disease: correlation between transmural healing and biomarkers
Authors: Castiglione Fabiana , Imperatore Nicola , Testa Anna , de Sire Roberto , Nardone Olga Maria , Ricciolino Simona , Di Luna Imma , Patturelli Marta , Villani Guido Daniele , Olmo Oriana , Rispo Antonio 
Published: July 2022

Background: While mucosal healing (MH) and transmural healing (TH) predict relevant clinical outcomes in Crohn’s disease (CD), little is known about the real significance and clinical impact of deep remission (DR). Objectives: To better explore the concept of DR, toward a direct correlation between MH, TH, and biomarkers. Design: Real-world observational longitudinal study to evaluate the rate of clinical remission (CR), MH and TH, and the fecal calprotectin (FC)/C-reactive protein (CRP) levels in all consecutive CD patients on biologics. Methods: A receiver operating characteristic (ROC) curve was constructed to define the best FC and CRP cut-offs associated with MH and TH. Finally, patients achieving CR, MH, and TH, in association with the target FC/CRP values, were considered in DR. Results: Among 118 CD patients, CR, MH, and TH were achieved in 62.7, 44.1, and 32.2%, respectively. After 2 years, the mean FC levels decreased from 494 ± 15.4 μg/g to 260 ± 354.9 μg/g ( p < 0.01). Using the ROC curve analysis, an FC cut-off value of 94 μg/g was associated with both MH [sensitivity: 94.2%, specificity: 84.8%, positive predictive value (PPV): 83.05%, negative predictive value (NPV): 94.92%, area under the curve (AUC): 0.95] and TH (sensitivity: 92.1%, specificity: 70%, PPV: 64.4%, NPV: 94.9%, AUC: 0.88). CRP < 5 mg/L was associated with both MH (sensitivity: 96.1%, specificity: 62.1%, PPV: 66.7%, NPV: 95.35%, AUC: 0.85) and TH (sensitivity: 97.4%, specificity: 52.5%, PPV: 52%, NPV: 95.35%, AUC: 0.78). When considering CD patients with concomitant CR, MH, and TH associated with an FC < 94 μg/g and CRP < 5 mg/L, this association was found identified in 33 patients (27.9%). Conclusion: An FC < 94 μg/g and a normal CRP are associated with CR, MH, and TH and could be included in the definition of DR in association. So by definition, DR could be achieved in approximately 30% of CD patients during maintenance treatment with biologics.
Tocilizumab in visual involvement of giant cell arteritis: a multicenter study of 471 patients
Authors: Loricera Javier , Castañeda Santos , Moriano Clara , Narváez Javier , Aldasoro Vicente , Maiz Olga , Melero Rafael , Villa Ignacio , Vela Paloma , Romero-Yuste Susana , Callejas José L. , de Miguel Eugenio , Galíndez-Agirregoikoa Eva , Sivera Francisca , Fernández-López Jesús C. , Galisteo Carles , Ferraz-Amaro Iván , Sánchez-Martín Julio , Sánchez-Bilbao Lara , Calderón-Goercke Mónica , Casado Alfonso , Hernández José L. , González-Gay Miguel A. , Blanco Ricardo 
Published: July 2022

Background: Visual involvement is the most feared complication of giant cell arteritis (GCA). Information on the efficacy of tocilizumab (TCZ) for this complication is scarce and controversial. Objective: We assessed a wide series of GCA treated with TCZ, to evaluate its role in the prevention of new visual complications and its efficacy when this manifestation was already present before the initiation of TCZ. Design: This is an observational multicenter study of patients with GCA treated with TCZ. Methods: Patients were divided into two subgroups according to the presence or absence of visual involvement before TCZ onset. Visual manifestations were classified into the following categories: transient visual loss (TVL), permanent visual loss (PVL), diplopia, and blurred vision. Results: Four hundred seventy-one GCA patients (mean age, 74 ± 9 years) were treated with TCZ. Visual manifestations were observed in 122 cases (26%), of which 81 were present at TCZ onset: PVL ( n = 60; unilateral/bilateral: 48/12), TVL ( n = 17; unilateral/bilateral: 11/6), diplopia ( n = 2), and blurred vision ( n = 2). None of the patients without previous visual involvement or with TVL had new episodes after initiation of TCZ, while only 11 out of 60 (18%) patients with PVL experienced some improvement. The two patients with diplopia and one of the two patients with blurred vision improved. Conclusion: TCZ may have a protective effect against the development of visual complications or new episodes of TVL in GCA. However, once PVL was established, only a few patients improved.
Intensive Olfactory Training in Post-COVID-19 Patients: A Multicenter Randomized Clinical Trial
Authors: Pires Ícaro de A. T. , Steffens Sara T. , Mocelin Aurenzo G. , Shibukawa Debora E. , Leahy Letícia , Saito Fernanda L. , Amadeu Nicole T. , Lopes Natália M. D. , Garcia Ellen C. D. , Albanese Maria L. , De Mari Laura F. , Ferreira Isabel M. , Veiga César A. , Jebahi Yasser , Coifman Herton , Fornazieri Marco A. , Hamerschmidt Rogério 
Published: July 2022

Background Olfactory dysfunction (OD)—including anosmia and hyposmia—is a common symptom of COVID-19. Previous studies have identified olfactory training (OT) as an important treatment for postinfectious OD; however, little is known about its benefits and optimizations after SARS-CoV-2 infection. Objective This study aimed to assess whether olfactory training performance can be optimized using more fragrances over a shorter period of time in patients with persistent OD after COVID-19. In addition, we determined the presence of other variables related to OD and treatment response in this population. Methods This multicenter randomized clinical trial recruited 80 patients with persistent OD and prior COVID-19 infection for less than 3 months. The patients were divided into 2 groups receiving either 4 or 8 essences over 4 weeks. Subjective assessments and the University of Pennsylvania Smell Identification Test (UPSIT) were performed before and after the treatment. Results Significant olfactory improvement was measured subjectively and using the UPSIT in both groups; however, no significant differences between the groups were observed. Additionally, the presence of olfactory fluctuations was associated with higher UPSIT scores. Conclusion These data suggest that training intensification by increasing the number of essences for 4 weeks does not show superiority over the classical method. Moreover, fluctuant olfaction seems to be related to a higher score on the UPSIT.
Decrease in Glycosaminoglycan with Aging in Normal Rat Articular Cartilage Is Greater in Females than in Males
Authors: Kobayashi-Miura Mikiko , Osago Harumi , Hamasaki Yoshifumi , Takano Ikuko , Akiho Mitsuki , Hiyoshi Mineyoshi , Hara Nobumasa 
Published: July 2022

Objective Osteoarthritis (OA) is more prevalent in females. We hypothesized that changes in articular cartilage (AC) constituents with aging may cause differences. Herein, we aimed to compare the changes in AC constituents with aging in male and female normal rats. Design The glycosaminoglycan (GAG) and collagen (COL) contents of the AC in knee, hip, and shoulder joints of male and female rats were quantified and compared between age groups and sexes. Results The amount of GAG was decreased in multiple joints in both males and females with aging. In females, it had a significant decrease in all joints measured. The decrease in GAG with aging was more severe in females than in males. Even in young rats, the amount of knee joint GAG was significantly less in females than in males. The amount of COL in the AC was unchanged with aging in both sexes. Conclusions The drastic GAG decrease with aging in female normal rats may explain the higher prevalence and more severe OA in females.
Instant messaging client gives the opportunity to recognize gut microbiota and dysbiosis-related disease: An investigation study on WeChat APP
Authors: Liu Yujie , Zhang Sheng , Wu Xia , Li Qianqian , Wang Yun , Huang Yihao , Zhang Faming , Cui Bota , Lu Xiang 
Published: July 2022

Objectives Gut microbiota and dysbiosis are closely related to the occurrence and development of various diseases. It is necessary to popularize gut microbiota-related knowledge to the public. And the instant messaging client on smartphones supplies a perfect tool to achieve this goal. Hence, we will describe the current status of gut microbiota education spread by WeChat official accounts. Methods The keywords of “gut microbiota,” “fecal microbiota transplantation (FMT),” and “probiotics” were searched in the articles published from January 2015 to August 2020 on the WeChat official accounts. And the data were analyzed based on the 10 common gut dysbiosis-related diseases. Results A total of 3061 WeChat official accounts have published 11,239 articles on gut microbiota dysbiosis-related diseases, with a rising trend in the total article numbers and the total pageviews. The keywords of “gut microbiota” dominate 50.61%, and the articles on inflammatory bowel disease had the largest proportion. Additionally, articles on the keyword “gut microbiota” also included cancer and obesity, articles on the keyword “FMT” mainly consist of Clostridium difficile infection and psychological disease, and the keyword “probiotics” was mainly related to obesity and irritable bowel syndrome disease. The top three total pageviews were on inflammatory bowel disease, obesity, and cancer. Conclusion This study indicates the current research hotspots and public concerns on the gut microbiota, and WeChat as an instant messaging client plays an important role in promoting the scientific popularization of gut microbiota.