bluuebunny/crossref_metadata_2025_split huggingface dataset:
A murine model of large-scale bone regeneration reveals a selective requirement for Sonic Hedgehog
Authors: Serowoky Maxwell A. , Kuwahara Stephanie T. , Liu Shuwan , Vakhshori Venus , Lieberman Jay R. , Mariani Francesca V. 
Published: May 2022

AbstractBuilding and maintaining skeletal tissue requires the activity of skeletal stem and progenitor cells (SSPCs). Following injury, local pools of these SSPCs become active and coordinate to build new cartilage and bone tissues. While recent studies have identified specific markers for these SSPCs, how they become activated in different injury contexts is not well-understood. Here, using a model of large-scale rib bone regeneration in mice, we demonstrate that the growth factor, Sonic Hedgehog (SHH), is an early and essential driver of large-scale bone healing.Shhexpression is broadly upregulated in the first few days following rib bone resection, and conditional knockout ofShhat early but not late post-injury stages severely inhibits cartilage callus formation and later bone regeneration. Whereas Smoothened (Smo), a key transmembrane component of the Hh pathway, is required in Sox9+ lineage cells for rib regeneration, we find thatShhis required in aPrrx1-expressing, Sox9-negative mesenchymal population. Intriguingly, upregulation ofShhexpression and requirements forShhandSmomay be unique to large-scale injuries, as they are dispensable for both complete rib and femur fracture repair. In addition, single-cell RNA sequencing of callus tissue from animals with deficient Hedgehog signaling reveals a depletion ofCxcl12-expressing cells, which may indicate failed recruitment ofCxcl12-expressing SSPCs during the regenerative response. These results reveal a mechanism by whichShhexpression in the local injury environment unleashes large-scale regenerative abilities in the murine rib.
Using open-source data to construct 20 metre resolution maps of children’s travel time to the nearest health facility
Authors: Watmough Gary R. , Hagdorn Magnus , Brumhead Jodie , Seth Sohan , Delamónica Enrique , Haddon Charlotte , Smith William C. 
Published: May 2022

AbstractPhysical access to health facilities is an important factor in determining treatment seeking behaviour and has implications for targets within the Sustainable Development Goals, including the right to health. The increased availability of high-resolution land cover and road data from satellite imagery offers opportunities for fine-grained estimations of physical access which can support delivery planning through the provision of more realistic estimates of travel times. The data presented here is of travel time to health facilities in Uganda, Zimbabwe, Tanzania, and Mozambique. Travel times have been calculated for different facility types in each country such as Dispensaries, Health Centres, Clinics and Hospitals. Cost allocation surfaces and travel times are provided for child walking speeds but can be altered easily to account for adult walking speeds and motorised transport. With a focus on Uganda, we describe the data and method and provide the travel maps, software and intermediate datasets for Uganda, Tanzania, Zimbabwe and Mozambique.
Physiological mechanisms of ABA-induced salinity tolerance in leaves and roots of rice
Authors: Chen Guanjie , Zheng Dianfeng , Feng Naijie , Zhou Hang , Mu Dewei , Zhao Liming , Shen Xuefeng , Rao Gangshun , Meng Fengyan , Huang Anqi 
Published: May 2022

AbstractAbscisic acid (ABA) plays a crucial role in response to abiotic stress as important small molecules in regulating metabolism. This study aimed to evaluate the ability of foliar spraying ABA to regulate growth quality at rice seedling stage under salt stress. Results demonstrated that salt stress strongly reduced all the growth parameters of two rice seedlings (‘Chaoyouqianhao’ and ‘Huanghuazhan’), caused prominent decrease in the levels of photosynthetic pigments (mainly in Huanghuazhan), photosynthesis and fluorescence parameters. Salinity treatment increased the concentration of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in roots, whereas significant decreased H2O2was found in leaves of Huanghuazhan. Additionally, salinity triggered high Na+content particularly in leaves and enhanced catalase (CAT) activities, ascorbate peroxidase (APX) and peroxidase (POD) activities of the two rice seedlings. Nevertheless, salinity-induced increased root ascorbic acid (AsA) and glutathione (GSH) levels while decreased in leaves, which depended on treatment time. Conversely, ABA application partially or completely mitigated salinity toxicity on the seedlings. ABA could reverse most of the changed physiological parameters triggered by salt stress. Specially, ABA treatment improved antioxidant enzyme levels and significantly reduced the Na+content of two varieties as well as increased the K+, Mg2+and Ca2+content in leaves and roots. ABA treatment increased the hormone contents of 1-aminocclopropane carboxylic acid (ACC), trans-zeatin (TZ), N6-isopentyladenosine (IPA), Indole-3-acetic acid (IAA), and ABA in leaves of two rice varieties under salt stress. It is suggested that ABA was beneficial to protect membrane lipid peroxidation, the modulation of antioxidant defense systems and endogenous hormonal balance with imposition to salt stress.
A monitoring survey and health risk assessment for pesticide residues on Codonopsis Radix in China
Authors: Wang Yanping , Han Jiabin , Zhang Jinjin , Li Xue , Bai Ruibin , Hu Fangdi 
Published: May 2022

AbstractIn recent years, the safety of Codonopsis Radix (CR) has attracted considerable attention. Pesticide residues is an important index to evaluate the safety of CR. The purpose of this study was to monitor pesticide residues in 164 batches of CR in China and assess dietary risk assessment. Firstly, a combined method of QuEChERS-GC–MS/MS and QuEChERS-LC–MS/MS was established for determination of 155 pesticide residues in CR. Second, 155 Pesticide residues in 3 CR cultivars from Gansu, Shanxi, Hubei, Guizhou and Chongqing were determined by this method. Finally, the risk score of pesticide residues in CR was evaluated, and the dietary health risk was evaluated based on the pesticide residues in CR. The results demonstrated that one or more pesticide residues were detected in 39 batches (23.78%) of 164 batches of CR. Of the 155 pesticide residues, 20 were detected. The most frequently detected pesticide residue was dimethomorph with a detection rate of 5.49%. Risk scores showed that 6 pesticides were at higher risk. Risk assessment based on the hazard quotient/hazard index (HQ/HI) approach revealed that exposure to pesticide residues which detected in CR were far below levels that might pose a health risk.
Natural extracts, honey, and propolis as human norovirus inhibitors
Authors: Ruoff Kerstin , Devant Jessica Michelle , Hansman Grant 
Published: May 2022

AbstractNorovirus is the most important cause of acute gastroenteritis, yet there are still no antivirals, vaccines, or treatments available. Several studies have shown that norovirus-specific monoclonal antibodies, Nanobodies, and natural extracts might function as inhibitors. Therefore, the objective of this study was to determine the antiviral potential of additional natural extracts, honeys, and propolis samples. Norovirus GII.4 and GII.10 virus-like particles (VLPs) were treated with different natural samples and analyzed for their ability to block VLP binding to histo-blood group antigens (HBGAs), which are important norovirus co-factors. Of the 21 natural samples screened, date syrup and one propolis sample showed promising blocking potential. Dynamic light scattering indicated that VLPs treated with the date syrup and propolis caused particle aggregation, which was confirmed using electron microscopy. Several honey samples also showed weaker HBGA blocking potential. Taken together, our results found that natural samples might function as norovirus inhibitors.
Purification, characterization, and determination of biological activities of water-soluble polysaccharides from Mahonia bealei
Authors: Kakar Mohib Ullah , Li Jingyi , Mehboob Muhammad Zubair , Sami Rokayya , Benajiba Nada , Ahmed Aziz , Nazir Amina , Deng Yulin , Li Bo , Dai Rongji 
Published: May 2022

AbstractMahonia bealeiis one of the important members of the genus Mahonia and Traditional Chinese Medicine (TCM). Several compounds isolated from this plant have exhibited useful biological activities. Polysaccharides, an important biomacromolecule have been underexplored in case ofM. bealei. In this study, hot water extraction and ethanol precipitation were used for the extraction of polysaccharides from the stem ofM. bealei, and then extract was purified using ultrafiltration membrane at 50,000 Da cut off value. Characterization of the purifiedM. bealeipolysaccharide (MBP) was performed using Fourier Transform Infrared Spectroscopy (FT-IR), along with Scanning Electron Microscopy (SEM), X-ray crystallography XRD analysis and Thermal gravimetric analysis (TGA). The purified polysaccharide MBP was tested for antioxidant potential by determining its reducing power, besides determining the DPPH, ABTS, superoxide radical, and hydroxyl radical scavenging along with ferrous ion chelating activities. An increased antioxidant activity of the polysaccharide was reported with increase in concentration (0.5 to 5 mg/ml) for all the parameters. Antimicrobial potential was determined against gram positive and gram-negative bacteria. 20 µg/ml MBP was found appropriate with 12 h incubation period againstEscherichia coliandBacillus subtilisbacteria. We conclude that polysaccharides fromM. bealeipossess potential ability of biological importance; however, more studies are required for elucidation of their structure and useful activities.
Changes in smell and taste perception related to COVID-19 infection: a case–control study
Authors: Cattaneo Camilla , Pagliarini Ella , Mambrini Sara Paola , Tortorici Elena , Mené Roberto , Torlasco Camilla , Perger Elisa , Parati Gianfranco , Bertoli Simona 
Published: May 2022

AbstractThe main aim of the present study was to psychophysically evaluate smell and taste functions in hospitalized COVID-19 patients and to compare those results with a group of healthy subjects. Another aim of the study was to assess the relationship of changes in patients’ smell and taste functions with a number of clinical parameters, symptoms, and other physiological signs as well as with severity of disease. Olfactory and gustatory functions were tested in 61 hospitalized patients positive for SARS-CoV-2 infection and in a control group of 54 healthy individuals. Overall, we found a significant impairment of olfactory and gustatory functions in COVID-19 patients compared with the control group. Indeed, about 45% of patients self-reported complaints about or loss of either olfactory or gustatory functions. These results were confirmed by psychophysical testing, which showed a significantly reduced performance in terms of intensity perception and identification ability for both taste and smell functions in COVID-19 patients. Furthermore, gustatory and olfactory impairments tended to be more evident in male patients suffering from more severe respiratory failure (i.e., pneumonia with need of respiratory support need during hospitalization).
Anemia warrants treatment to improve survival in patients with heart failure receiving sacubitril–valsartan
Authors: Yang Tsun-Yu , Lee Chii-Ming , Wang Shih-Rong , Cheng Yu-Yang , Weng Shao-En , Hsu Wan-Tseng 
Published: May 2022

AbstractAngiotensin inhibition remains a cornerstone for pharmacologic management of heart failure (HF), despite being associated with decreased hemoglobin (Hb) levels. To investigate the effect of anemia and its treatment on patients with HF treated with sacubitril–valsartan (S/V), we conducted a retrospective study involving patients with recorded left ventricular ejection fractions (LVEFs) of < 40% between January 2017 and December 2019. We identified 677 patients, 37.7% of whom received S/V. The median follow-up period was 868 days. Anemia was associated with significantly decreased survival, increased mortality rates, and higher all-cause hospitalizations in S/V-using patients. We further analyzed 236 patients with HF who had recorded renal function, LVEF, and Hb at the initiation of S/V therapy to identify Hb patterns after S/V therapy. Of these patients, 35.6% exhibited decreasing Hb 12 months after S/V initiation, which was associated with a lower survival rate. Among the patients who were not prescribed anemia medications, Hb of ≥ 12 (vs. < 12 g/dL) was associated with a higher survival rate; this association was absent among the patients undergoing anemia treatment. These results emphasize that consistent screening and treatment for anemia should be implemented to reduce the morbidity and mortality of patients with HF receiving S/V.
Cisterna chyli as an optimal marker of tolvaptan response in severe cirrhotic ascites
Authors: Hirooka Masashi , Koizumi Yohei , Yano Ryo , Nakamura Yoshiko , Sunago Koutarou , Yukimoto Atsushi , Watanabe Takao , Yoshida Osamu , Tokumoto Yoshio , Abe Masanori , Hiasa Yoichi 
Published: May 2022

AbstractFor patients with cirrhosis, no definitive predictor of the efficacy and prognosis of tolvaptan treatment exists. We assessed the cisterna chyli’s utility as an optimal marker. We retrospectively enrolled 172 patients with cirrhosis. The effect of tolvaptan was evaluated using post-treatment survival time. The overall response to tolvaptan was 52.3%. The median cisterna chyli diameter was 4.1 mm. Of 172 patients, 100 were included in the pilot set and 72 in the validation set. According to the Youden index, the cisterna chyli diameter’s cutoff value was 4 mm, with a sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, and negative likelihood ratio of 92%, 83%, 86%, 91%, 5.43, and 0.09, respectively, in the pilot set. The area under the curve of the cisterna chyli diameter for evaluating tolvaptan’s effect was 0.911 and 0.988 in the pilot and validation sets, respectively. During multivariate analysis, cisterna chyli narrowing and furosemide treatment were significant predictive factors for tolvaptan’s insufficient effect. Cumulative liver transplantation-free survival rates were significantly higher in patients with cisterna chyli dilatation than in those without (p = 0.028). Our findings suggest a strong association of cisterna chyli with tolvaptan treatment response in patients with cirrhosis and hepatic edema.
On the round number bias and wisdom of crowds in different response formats for numerical estimation
Authors: Honda Hidehito , Kagawa Rina , Shirasuna Masaru 
Published: May 2022

AbstractWhen asked for numerical estimations, people can respond by stating their estimates (e.g., writing down a number) or indicating a number on a scale. Although these methods are logically the same, such differences may affect the responses to the numerical estimations. In this study, we examined how differences in response format affected responses to numerical estimations using two behavioral experiments. We found that participants showed a round number bias (i.e., people answered estimates with round numbers) when simply stating a number and the distribution of responses tended to be less diverse. In contrast, this tendency was not observed when the participants responded using a scale. Participants provided more diverse estimates when they answered using a scale. Furthermore, we analyzed how this difference in response distribution was related to the wisdom of crowds (the aggregated judgment is as accurate as, or sometimes better than, the best individual judgment in the group) using computer simulations. The results indicated that round number bias affected the achievement of the wisdom of crowds. Particularly, when the group size was small, biased responses resulted in less effective achievement. Our findings suggest that using an appropriate scale is a low-cost method for eliminating round number bias and efficiently achieving the wisdom of crowds.
Low-intensity low-frequency pulsed ultrasound ameliorates sciatic nerve dysfunction in a rat model of cisplatin-induced peripheral neuropathy
Authors: Bilir-Yildiz Busra , Sunay Fatma Bahar , Yilmaz Hatice Fulya , Bozkurt-Girit Ozlem 
Published: May 2022

AbstractChemotherapy-induced peripheral neuropathy is a neurological complication that frequently occurs during chemotherapeutic intervention, resulting in damaged myelin sheath, motor weakness and/or sensory impairment. This study aims to investigate the therapeutic efficiency of low-intensity pulsed low-frequency ultrasound on cisplatin-induced peripheral neuropathy. Rats were randomly divided into five experimental groups as control, cisplatin administration, 10 mg/kg melatonin treatment after cisplatin administration, 1 MHz frequency 0.5 W/cm2 pulsed ultrasound treatment after cisplatin administration and 1 MHz frequency 1.5 W/cm2 pulsed ultrasound treatment after cisplatin administration. Chemical neuropathy was induced by the injection of 3 mg/kg/week of cisplatin (i.p.) for 5 weeks. Afterwards, melatonin and pulsed ultrasound treatments were applied for 15 consecutive days. Cisplatin administration resulted in a decrease in nociceptive pain perception and nerve conduction velocities together with a decrease in myelin thickness and diameters of axons and myelinated fibers, indicating a dysfunction and degeneration in sciatic nerves. In addition, cisplatin administration led to a decrease, in superoxide dismutase activity, and an increase in malondialdehyde and IL-1β levels together with an increase in caspase-3 protein expression levels and a decrease in Bcl-2 and Parkin levels. The ultrasound treatments resulted in an increase in nociceptive pain perception and sciatic nerve conduction; led to a decrease in oxidative stress and inflammation, restored nerve degeneration and regulated apoptosis and mitophagy. Taken together, low-intensity pulsed low-frequency ultrasound was efficient in restoring the alterations attributable to cisplatin-induced peripheral neuropathy, and warrants further investigations.
Isolate sets partition benefits community detection of parallel Louvain method
Authors: Qie Hang , Li Shijie , Dou Yong , Xu Jinwei , Xiong Yunsheng , Gao Zikai 
Published: May 2022

AbstractCommunity detection is a vital task in many fields, such as social networks, and financial analysis, to name a few. The Louvain method, the main workhorse of community detection, is a popular heuristic method based on modularity. But it is difficult for the sequential Louvain method to deal with large-scale graphs. In order to overcome the drawback, researchers have proposed several parallel Louvain methods (Parallel Louvain Method, PLM), which suffer two challenges: (1) latency in the information synchronization and (2) communities swap. To tackle these two challenges, we propose a graph partition algorithm for the parallel Louvain method. Different from existing graph partition algorithms, our graph partition algorithm divides the graph into subgraphs called isolate sets, in which vertices are relatively decoupled from others, and the PLM computes and synchronizes information without delay and communities swap. We first describe concepts and properties of isolate sets. In the second place, we propose an algorithm to divide the graph into isolate sets, which enjoys the same computation complexity as the breadth-first search. Finally, we propose the isolate-set-based parallel Louvain method, which calculates and updates vertices information without latency and communities swap. We implement our method with OpenMP on an 8-cores PC. Experiments on 18 graphs show that our parallel method achieves a maximum 4.62 $$\times $$
×
speedup compared with the sequential method, and outputs higher modularity on 14 graphs.
Modeling of wave run-up by applying integrated models of group method of data handling
Authors: Mahdavi-Meymand Amin , Zounemat-Kermani Mohammad , Sulisz Wojciech , Silva Rodolfo 
Published: May 2022

AbstractWave-induced inundation in coastal zones is a serious problem for residents. Accurate prediction of wave run-up height is a complex phenomenon in coastal engineering. In this study, several machine learning (ML) models are developed to simulate wave run-up height. The developed methods are based on optimization techniques employing the group method of data handling (GMDH). The invasive weed optimization (IWO), firefly algorithm (FA), teaching–learning-based optimization (TLBO), harmony search (HS), and differential evolution (DE) meta-heuristic optimization algorithms are embedded with the GMDH to yield better feasible optimization. Preliminary results indicate that the developed ML models are robust tools for modeling the wave run-up height. All ML models’ accuracies are higher than empirical relations. The obtained results show that employing heuristic methods enhances the accuracy of the standard GMDH model. As such, the FA, IWO, DE, TLBO, and HS improve theRMSEcriterion of the standard GMDH by the rate of 47.5%, 44.7%, 24.1%, 41.1%, and 34.3%, respectively. The GMDH-FA and GMDH-IWO are recommended for applications in coastal engineering.
Delayed processing of blood samples impairs the accuracy of mRNA-based biomarkers
Authors: Wilson Chace , Dias Nicholas W. , Pancini Stefania , Mercadante Vitor , Biase Fernando H. 
Published: May 2022

AbstractThe transcriptome of peripheral white blood cells (PWBCs) are indicators of an organism’s physiological state, thus making them a prime biological sample for mRNA-based biomarker discovery. Here, we designed an experiment to evaluate the impact of delayed processing of whole blood samples on gene transcript abundance in PWBCs. We hypothesized that storing blood samples for 24 h at 4 °C would cause RNA degradation resulting in altered transcriptome profiles. There were no statistical differences in RNA quality parameters among samples processed after one, three, six, or eight hours post collection. Additionally, no significant differences were noted in RNA quality parameters or gene transcript abundance between samples collected from the jugular and coccygeal veins. However, samples processed after 24 h of storage had a lower RNA integrity number value (P = 0.03) in comparison to those processed after one hour of storage. Using RNA-sequencing, we identified four and 515 genes with differential transcript abundance in samples processed after storage for eight and 24 h, respectively, relative to samples processed after one hour. Sequencing coverage of transcripts was similar between samples from the 24-h and one-hour groups, thus showing no indication of RNA degradation. This alteration in transcriptome profiles can impair the accuracy of mRNA-based biomarkers, therefore, blood samples collected for mRNA-based biomarker discovery should be refrigerated immediately and processed within six hours post-sampling.