bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translational profiling of neuronal subtypes in pre-symptomatic fatal familial insomnia mice reveals TOR signaling in somatostatin-expressing neurons
Authors: Bauer Susanne , Dittrich Lars , Kaczmarczyk Lech , Schleif Melvin , Benfeitas Rui , Jackson Walker S. 
Published: March 2022

Abstract
BackgroundSelective neuronal vulnerability is a common, yet poorly understood characteristic of neurodegenerative diseases. It is particularly fascinating in familial prion diseases, such as fatal familial insomnia (FFI) and Creutzfeldt-Jakob disease (CJD), where different mutants of the prion protein manifest as clinically and neuropathologically distinct diseases. MethodsTo determine how distinct neurons respond to different mutations in the prion protein gene at pre-symptomatic stages, we used RiboTag to isolate cell type-specific, translating mRNA from GABAergic, glutamatergic, somatostatin- (SST) and parvalbumin- (PV) expressing neurons of 9-month-old knock-in mouse models of FFI and CJD. Differential gene expression analysis followed by gene set enrichment analysis (GSEA) was performed for all cell types in both diseases. We further constructed a undirected weighted gene co-expression network for SST neurons to identify functional models and hub genes.ResultsWe found SST+ neurons showed the most prominent gene expression changes in both diseases, especially in FFI, with high similarities between the two diseases. GSEA demonstrated similar enrichment patterns of functional terms for GABAergic cell types in both FFI and CJD, whereas responses in glutamatergic neurons were disease specific. For SST+ neurons, functional analysis revealed upregulation of ribosomal biogenesis, mitochondrial function and neurodegenerative disease pathways, and downregulation of synaptic function and small GTPase mediated signaling in FFI. Analysis of an SST co-expression network revealed a disease-associated module, functionally associated with autophagy and TORC1 signaling. Of the identified module hub genes, three were further significantly differentially expressed in FFI SST neurons, including Depdc5, a component of mTOR regulator complex GATOR1, and Nucleosome Remodeling Deacetylase complex component Mta3. Importantly, the molecular changes reported here were very different from those reported for an acquired prion disease model.ConclusionsThis study identifies SST neurons as an early affected cell type, showing similar responses in both FFI and CJD with downregulation of mTOR signaling as a potential explanatory mechanism underlying many of the observed changes. The observation that FFI and CJD have such similar cellular and molecular signatures indicates that a common therapy may be effective for multiple inherited prion diseases, but possibly less effective for acquired prion disease.
Effects of warm-up exercises with dynamic stretching and total-resistance exercise (TRX) suspension for enhancing core body temperature and the metabolic equivalents of task of body extremities on Crawl stroke speed in young athlete swimmers with different BMI classifications
Authors: Pengkumpa Sittichai , Sangpara Paramaporn , Konharn Kurusart 
Published: March 2022

Abstract
Purpose We aimed to compare the effects of warm-up exercises with dynamic stretching and total-resistance exercise (TRX) suspension for enhancing core body temperature and the metabolic equivalents of task of body extremities on Crawl stroke speed in young athlete swimmers with different body mass index (BMI) classifications. Methods The sample comprised 32 young athlete swimmers (age 13.74 ± 1.03 years, BMI 19.91 ± 3.34 kg/m2) who had been involved in organized competitive swimming. BMI was divided into 3 classifications. After a conventional warm-up and 10-mins rested, the intervention group were received the TRX warm-up protocol, while the control group performed with dynamic stretching. They then performed a 50-meters and a 100-meters crawl-stroke for speed. Before completing the testing, swimmers had a one-day rest period for their own activities. Results Warm-ups with dynamic stretching affect the core body temperature, heart rate, and metabolic equivalent of task (METs rate), and enhance crawl-stroke speed performance in young athlete swimmers. While swimmers in BMI class 2 have shown the best crawl-stroke speed, warm-ups with TRX suspension required lower energy expenditures (METs rate) than warm-ups with dynamic stretching (P<0.01). Male swimmers were found to have faster crawl-stroke speed performances than female swimmers in warm-ups with TRX suspension and dynamic stretching. Conclusion Energy expenditure, body temperature, and heart rate in the warm-up protocol have correlations with swimming performance.
Chemotherapy may facilitate colorectal cancer metastasis through vessel formation by upregulating G-CSF
Authors: zhan Yueping , Fang Kai , wang Yuqian , Liang Mengting , Wu Ruiying , Xu Jian , Yuan Zeting , Wu Chengqi , Zhu Ruiqiu , Xu Jianhua , Chen Teng , Wang Jie , Zhang Jun , Yin Peihao , Liang Xin , Xu Ke 
Published: March 2022

Abstract
Background
Chemotherapy remains the mainstay of treatment for various cancers. However recent studies suggested that it may also induce local or systemic changes that promote the dissemination and proliferation of cancer cells leading to successful metastasis. Understanding this process is therefore instrumental to help us identify patient population that may or may not be benefit from chemotherapy, and develop innovative approaches to prevent metastasis.
Methods
In this study, we investigated the mechanism of chemotherapy causing the metastasis of colorectal cancer, and the roles of G-CSF induced by chemotherapy in the vessel formation. Eight-week-old C57/BL6 mice were used as animal models in this study to investigate the lung metastasis by the chemotherapy. Conditional endothelial cell STAT3−/− (Signal Transducer and Activator of Transcription, STAT) knockout mice (STAT3flox/flox; Tek-Cre mice), were used to investigate the function of STAT3 on lung metastasis under the chemotherapy. Bone marrow and plasma was selected to detect the mobilization of endothelial progenitor cells (EPCs) and MDSCs. To trace BM-derived cells, we prepared chimeric mouse transplanted with BM cells from green fluorescent protein (GFP) transgenic mouse. In vitro, experiments were performed in human umbilical vein endothelial cells (HUVEC) to analyse the function of G-CSF on angiogenesis. Detected the G-CSF content in the plasma of the patients who received an adjuvant chemotherapy with the XELOX (oxaliplatin plus capecitabine) regimen.
Results
Our study showed that oxaliplatin chemotherapy could increase the expression of G-CSF to promote lung metastasis. First, G-CSF/STAT3 signaling facilitated lung metastasis by enhancing vascular adhesion rather than diminishing the blood vessel density. G-CSF also promoted Endothelial Progenitor Cells (EPCs) mobilization that devoted to vasculogenesis, a critical step for vessel density in metastatic sites; moreover, chemotherapy augmented the mobilization of MDSCs from the bone marrow by G-CSF. In consistent with these, anti-G-CSF suppressed the formation of a functional vasculature and induced tumoral immunosuppression, resulting in an anti-metastasis effect during chemotherapy. Furthermore, at human level, we observed high level of G-CSF, either at baseline or after receiving adjuvant XELOX chemotherapy, correlated with poor overall and recurrence-free survival.
Conclusions
These results demonstrate that certain chemotherapy could paradoxically result in worse outcome due to increased expression of G-CSF. Our findings provide mechanistic insight into cautious use of G-CSF and potential utility of anti-G-CSF in personalized cancer therapy.
Molecular dynamics and docking studies on potentially active natural phytochemicals for targeting SARS-CoV-2 Main Protease
Authors: KS Sandhya , Nair Achuthsankar S. 
Published: March 2022

Abstract
In this study, we screened eighty seven novel phytochemical compounds and identified the best for targeting the main protease (Mpro) receptor of SARS-CoV-2. Interestingly, the studied phytochemicals are present in four natural herbs namely, Aegle Marmelos, Coleus Amboinicus, Aerva Lanta and Biophytum Sensitivum. After categorizing all the phytochemicals based upon LibDock scores, we identified six compounds with scores over 120, namely, Ervoside, Epoxyaurapten, Epicatechin, Feruloyltyramine, Marmin and Aegelinosides B. Among them Aegelinosides B leads with a very high LibDock value of 142.50 (binding energy: -8.54 kcal/mol). We also made molecular dynamics simulations for the best six systems and explored their structural stability (RMSD), Cα fluctuations (RMSF), intermolecular hydrogen bond interactions, effect of solvent accessibility (SASA) and compactness (Rg) factors. Satisfactory ADMET and druglikeness features were found for all these compounds and we therefore strongly propose the initiation of trial studies on these compounds for fighting SARS-CoV-2.
Magnetic, Synergistic interaction of SnO2/ZnFe2O4 nanocomposite participated catalytic degradation performance of coordination complex under UV illumination
Authors: D Venkatesh , R Silambarasan , K Anbalagan 
Published: March 2022

Abstract
Magnetic, cost-effective heterojunction SnO2/ZnFe2O4 nanocomposites synthesized through simple one-pot hydrothermal technique and its phase formation, microstructure, morphology, chemical states and magnetism examined by various characteristics. The photoconversion of mixed ligand involved cis-[CoIII(en)2(Im)Cl]Cl2 by SnO2/ZnFe2O4 nanocomposite showed efficient catalytic activity compared to the bare SnO2 and ZnFe2O4 nanoparticles under the wavelength of 254 nm and 365 nm in water and binary solvents system. SnO2/ZnFe2O4 heterojunction photocatalyst shown enhanced electron donor-acceptor capacitance, excellent suppression of recombination of e-/h+ pairs and efficient generation of photogenerated reactive species. The rate constant (k) of SnO2/ZnFe2O4 (0.0540 sec-1) is higher than the SnO2 (0.0160 sec-1) and ZnFe2O4 (0.157 sec-1) in H2O:PriOH (70:30%) than remain solvents system in catalytic reduction of complex. The photoactive mechanism of cis-[CoIII(en)2(Im)Cl]Cl2 by SnO2/ZnFe2O4 nanocomposite and its corresponding bare nanoparticles were discussed in detail.
Genetic etiology and obstetric outcome analysis of fetal cystic hygroma in a single-center study
Authors: Cai Meiying , lin min , su Linjuan , wu Xiaoqing , xie Xiaorui , ji Qingqiang , xu liangpu , lin na , huang Hailong 
Published: March 2022

Abstract
Cystic hygroma (CH) is a somewhat common observation in prenatal ultrasounds; however, there is no consensus regarding the clinical management of fetuses with CH. A retrospective analysis was performed on 40 pregnant patients whose fetuses had CH from November 2016 to June 2021. Villus, amniotic fluid, or umbilical cord blood samples were collected according to the corresponding gestational age for karyotype analysis and single-nucleotide polymorphism array (SNP-array). Among the 40 fetuses with CH, 16 (40.0%, 16/40) exhibited isolated CH and 24 (60.0%, 24/40) exhibited CH combined with other ultrasound abnormalities. The most common CH-comorbid ultrasound abnormalities in this study were congenital heart disease (25.0%, 6/24), followed by thickened nuchal translucency (20.8%, 5/24), and fetal edema (12.5%, 3/24). After karyotype and SNP-array analysis, an overall detection rate of 30.0% (12/40) was achieved. Using karyotype analysis, eight cases of pathogenic copy number variations (CNVs) were detected, among which 45, X was the most common. In addition to the above pathogenic CNV, four additional cases of pathogenic CNVs were detected by SNP-array. There was no significant difference in pathogenic CNVs of isolated CH and CH combined with other ultrasound (31.3% vs. 29.2%, P = 1). Karyotype analysis and SNP-array results influence whether fetal parents terminate pregnancy. When genetic abnormalities are detected in the fetus, the parents often choose to terminate the pregnancy. Genomic examination should be performed on fetuses with CH to confirm the etiology as soon as possible. During genetic counseling, all fetal characteristics should be carefully and comprehensively evaluated.
OptNCMiner: A deep learning approach for the discovery of natural compounds modulating disease-specific multi-targets
Authors: Shin Seo Hyun , Oh Seung Man , Park Jung Han Yoon , Lee Ki Won , Yang Hee 
Published: March 2022

Abstract
Background: Due to their diverse bioactivity, natural product (NP)s have been developed as commercial products in the pharmaceutical, food and cosmetic sectors as natural compound (NC)s and in the form of extracts. Following administration, NCs typically interact with multiple target proteins to elicit their effects. Various machine learning models have been developed to predict multi-target modulating NCs with desired physiological effects. However, due to deficiencies with existing chemical-protein interaction datasets, which are mostly single-labeled and limited, the existing models struggle to predict new chemical-protein interactions. New techniques are needed to overcome these limitations.Results: We propose a novel NC discovery model called OptNCMiner that offers various advantages. The model is trained via end-to-end learning with a feature extraction step implemented, and it predicts multi-target modulating NCs through multi-label learning. In addition, it offers a few-shot learning approach to predict NC-protein interactions using a small training dataset. OptNCMiner achieved better prediction performance in terms of recall than conventional classification models. It was tested for the prediction of NC-protein interactions using small datasets and for a use case scenario to identify multi-target modulating NCs for type 2 diabetes mellitus complications.Conclusions: OptNCMiner identifies NCs that modulate multiple target proteins, which facilitates the discovery and the understanding of biological activity of novel NCs with desirable health benefits.
Production of sealed rod sources made from epoxy resin for the Saint-Petersburg brick phantom for the calibration of whole-body counters
Authors: Woidy Patrick , Meisenberg Oliver 
Published: March 2022

Abstract
Rod sources are a common tool for the calibration of whole-body counters in combination with the Saint-Petersburg brick phantom. A method for the production of such sources in ordinary radiochemical laboratories is presented. The sources consist of a tubular capsule of rigid polyvinyl chloride with a radioactive filling of epoxy resin. The method allows the production of sources at material costs of 1 € per source and of 10 sources by one person per day. Quality-assurance measurements were performed regarding the spatial distribution of the activity within the sources and the distribution of the activity throughout a set of sources. The relative double standard deviation of the activities of 5 different segments of single sources was 7.1%. The relative double standard deviation within a set of 90 sources was 2.8% after those 11% of sources with the greatest deviation from the arithmetic mean were discarded. Tests according to ISO 2919 to certify the sources as sealed sources of Class 2 of this standard were successfully conducted. The bending test proved to be the most critical test for the sources; the sources were broken by a mass of 12 to 14 kg, which is only slightly more than the stipulated mass of 10.2 kg.
Clinical Evaluation of The Efficacy of Femtosecond Laser-Assisted Anterior Lamellar Keratoplasty with Long-Term Follow-Up.
Authors: Wylęgała Adam , Kokot Joanna , Roszkowska Anna M , Dobrowolski Dariusz , Wylęgała Edward 
Published: March 2022

Abstract
Introduction: To evaluate the clinical outcome, efficacy, and safety of femtosecond laser-assisted anterior lamellar keratoplasty. Material and Methods: In this prospective study, 32 patients (21M and 10F) aged from 15 to 62 years (mean 38.5) with different pathologies of the anterior corneal layers were enrolled for anterior lamellar keratoplasty using femtosecond laser VisuMax (Carl Zeiss, Germany). All patients were examined for uncorrected (UCVA) and best-corrected (BCVA) distance and near visual acuity, astigmatism, endothelial cell density, corneal thickness, and intraocular pressure. These examinations were performed before transplantation, at hospital discharge, and after 3 and 12 months. The mean follow-up time was 65.36 ± 28.54 months. Results: Statistically significant improvement of both UCVA and BCVA for distance and near vision was registered. BCVA improved from 0.11 preoperatively to 0.168 (p = 0.03), 0.267 (p < 0.01) and 0.472 (p < 0.01) on the hospital discharge day, 3 months and 12 months respectively. There were no statistically significant differences in astigmatism, intraocular pressure, endothelial cell density, and corneal pachymetry as compared preoperative and postoperative values. Six patients (19%) had a graft failure with a rate of 33% at 26 months. Conclusions: Femtosecond laser-assisted anterior lamellar keratoplasty is a safe and effective surgical method, providing satisfactory graft survival rates.
Long non-coding RNA HOTAIR regulates the stemness of breast cancer cells via activation of NF-κB signaling pathway
Authors: Wang Jiajia , Liu Xingzhu , Li Ping , Wang Junrong , Shu Yu , Zhong Xinyu , Gao Zhen , Yang Jingyi , Jiang Yashuang , Yang Geng 
Published: March 2022

Abstract
Background: Breast cancer is the most prevalent common disease among women, and it is characterized by a high rate of tumor development and heterogeneity. Breast cancer stem cells (CSCs) may well contribute to the these pathological properties, but the mechanisms underlying their self-renewal and maintenance are still elusive. Methods: Breast cancer stem cells (BCSCs) were isolated using flow cytometry. Both in vitro and in vivo functional assays were performed to investigate the role of HOTAIR in tumorigenic activity and stemness maintenance of BCSCs. Real-time PCR, Western blot, RNA pull down, RNA immunoprecipitation (RIP) and ChIP assays were carried out to reveal the pathways that how HOTAIR regulated stemness of BCSCs through NF-κB pathway. Results: Here, we discover the long noncoding RNA HOTAIR that is highly expressed in breast CSCs. HOTAIR is required for breast CSC self-renewal and tumor propagation. Mechanistically, HOTAIR recruits the PRC2 to the promoter of IκBα to inhibit its expression, leading to activation of NF-κB signaling pathway. The activated NF-κB signaling promotes downstream c-Myc and Cyclin D1 expression. The IκBα level, as well as the survival rate of patients, with high HOTAIR expression was significantly lower than that of patients with relatively low HOTAIR in clinical samples. Our data suggest that HOTAIR-mediated NF-κB signaling primes breast CSC self-renewal and tumor propagation. Conclusions: In sum, we have identified HOTAIR-based NF-κB signaling regulatory circuit that promotes tumorigenic activity in breast cancer stem cells, further indicating that HOTAIR could be a promising target for clinical treatment of breast cancers.
Associations of dietary folate, vitamin B6 and B12 intake with cardiovascular outcomes in 115664 participants: a large UK population-based cohort
Authors: Zhang Bo-Ya , Dong Hao-Yu , Xu Ying , Xu Duo , Sun Hong-peng , Han Li-yuan 
Published: March 2022

Abstract
Background & Aims
: The evidence of relationship between dietary intake of folate, vitamin B6 and vitamin B12 and cardiovascular diseases(CVD) in UK populations is limited. We aimed to analyze the association of dietary intake of folate, vitamin B6, and vitamin B12 with CVD events [stroke, myocardial infarction(MI)] and CVD mortality.
Methods
We included 115664 participants, aged 40–70 years, with no CVD events or cancer at baseline, enrolled between 2006 and 2010 and followed up to the end of 2018. Dietary intake was measured with an online 24-hour dietary assessment. Multivariable Cox proportional hazards models were used to estimate hazard ratios (HR) and 95% confidence intervals (CI) for the associations.
Results
After multivariate adjustment, higher dietary folate intake was associated with a lower risk of CVD events [HR per standard deviation(SD) 0.95, 95% CI 0.93 to 0.98], stroke (HR per SD 0.95, 95% CI 0.91 to 0.99), MI(HR per SD 0.94, 95% CI 0.91 to 0.98),CVD mortality(HR per SD 0.90, 95% CI 0.83 to 0.97). Each tablespoons/day higher intake of raw vegetable intake, pieces/day higher intake of fresh fruit intake and bowls/week higher intake of cereal intake were associated with higher intakes of of folate every 0.02,0.06 and 0.05 SD, respectively. E-value analysis suggested robustness to unmeasured confounding.
Conclusion
Each increase in folate intakes was related to 5% lower risks of total CVD events and 10% lower risks of CVD mortality. Our findings support that strengthening dietary folate intake as a primary prevention strategy for CVD events and CVD mortality.
Effect of elevated ozone on soybean (Glycine max L.) cultivar: Role of orange juice and synthetic ascorbic acid
Authors: Chaudhary Indra Jeet , Nigam Bhavna , Rathore Dheeraj 
Published: March 2022

Abstract
Ozone is a kind of hazardous gas for the environment and negatively affects the plant and human health. These days, phytoextracts are commonly used as a source of bioactive compounds for reducing the detrimental environmental effects on plants. In presented study, soybean cultivar JS-335 was used for assessment of protective role of synthetic ascorbic acid (SAA) and orange juice (25 % orange juice, enriched ascorbic acid) under ozone stress conditions. The results showed that under ozone stress soybean cultivar JS-335 reduced growth, biomass and also negatively affects the biochemical properties of plants due to these changes finally caused yield losses. Foliar-applied OJ >and SAA were found to be very effective in improving plant growth and development and also increased the yield of the crop. It was discovered that a 25% OJ coupled with ascorbic acid and other essential nutrients and biomolecules was almost as effective as a 100 ppm SAA in reducing the harmful effects of ozone stress on soybean plants. As a result, it was determined that OJ, a less expensive source of ascorbic acid, can be used to improve ozone resistance in plants in ozone-prone areas.
Comparison of LSM Indexing Techniques for Storing Spatial Data
Authors: Mao Qizhong , Qader Mohiuddin Abdul , Hristidis Vagelis 
Published: March 2022

Abstract
In the pre-big data era, many traditional databases supported spatial queries via spatial indexes. However, modern applications are seeing a rapid increase of the volume and ingestion rate of spatial data. LSM tree is used by many big data systems as their storage structure in order to support write-intensive large-volume workloads, which are usually only optimized for single-dimensional data. Research has studied how spatial indexes can be supported on LSM systems, but focused mainly on the local index organization, that is, how data is organized inside a single LSM component. In this paper, we study various aspects of LSM spatial indexing, including spatial merge policies, which determine when and how spatial components are merged. We consider three stack-based and one leveled merge policies, which we have implemented on the same big data system Apache AsterixDB. We evaluate the write and read performance on various workloads and discuss our findings and recommendations. A key finding is that Leveled policies are under-performing other stack-based merge policies for most types of spatial workloads.