bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying the Role of NUDCD1 in Human Tumors from Clinical and Molecular Mechanisms: A Study Based On Comprehensive Bioinformatics and Experimental Validation
Authors: Han Bin , He Jinsong , Li Yuanting , Chen Qing , Yuan Min , Zeng Xi , Zeng Yan , He Meibo , Feng Dan , Ma Daiyuan 
Published: November 2022

Abstract
Purpose
Cancer is a major public health condition characterized by high morbidity and mortality. NUDCD1 (NudC domain-containing 1) is abnormally activated in multiple tumors and has been identified as a cancer antigen. But there is still no pan-cancer analysis available for NUDCD1 in human cancers.
Methods
The role of NUDCD1 across multiple tumors was explored using data from the public databases including HPA, TCGA, GEO, GTEx, TIMER2, TISIDB, UALCAN, GEPIA2, cBioPortal, GSCA and so on. Molecular experiments (e.g., quantitative real-time PCR, immunohistochemistry and western blot) were conducted to validate the expression and biological function of NUDCD1 in STAD tissues and cells.
Results
NUDCD1 was highly expressed in most tumors and its levels were associated with the prognosis of cancer patients. Multiple genetic and epigenetic features of NUDCD1 exist in different cancers such as uterine corpus endometrial carcinoma or kidney renal papillary cell carcinoma. NUDCD1 was associated with expression levels of recognized immune checkpoints (anti-CTLA-4) and immune infiltrates (e.g., CD4 + and CD8 + T cells) in some cancers. Moreover, NUDCD1 correlated with the CTRP and GDSC drug sensitivity and acted as a link between chemicals and cancers. The functional mechanisms of NUDCD1 included RNA metabolism- and protein processing-associated functions. Importantly, NUDCD1-related genes were enriched in several tumors (e.g., COAD, STAD and ESCA) and affected apoptosis, cell cycle and DNA damage cancer-related pathways. Furthermore, expression, mutation and copy number variations for the gene sets were also associated with prognosis. At last, we experimentally validated the overexpression of NUDCD1 in STAD clinical samples and determined its contribution to STAD in vitro and in vivo.
Conclusions
NUDCD1 was involved in diverse biological processes and it influenced the occurrence and development of cancers through diverse underlying mechanism. This first pan-cancer analysis for NUDCD1 provides a comprehensive understanding about its roles across various cancer types, especially in STAD.
Optical coherence tomography in patients with Wilson’s disease: A single center, prospective study
Authors: Ning Wei Qin , Lyu Chun Xiao , Diao Sheng-Peng , Huang Ye-Qing , Liu Ai-Qun , Yu Qing-Yun , Hong Ming-Fan , Peng Zhong-Xing , Zhou ZhiHua 
Published: November 2022

Abstract
Background: Morphological changes of retina in patients with Wilson’s disease
(WD) can be found by optical coherence tomography (OCT), and such changes have significant differences between neurological forms（NWD ) and hepatic forms (HWD) of WD. We aimed to evaluate the relationship between morphological parameters of retina and brain magnetic resonance imaging (MRI) changes, course of disease, type of disease and sexuality in WD. This is a single center, prospective study including forty-six WD patients and forty healthy controls (HC). We employed theGlobal Assessment Scale (GAS) to assess the the neurological sign of WD patients.
Results: NWD had thinner superior parafovea zone (108.07±6.89 um vs. 114.40±5.54 um, p<0.01), temporal parafovea zone (97.17±6.65 um vs. 103.60±4.53 um, p<0.01), inferior parafovea zone (108.114±7.65 um vs. 114.93±5.84 um, p<0.01) nasal parafovea zone (105.53±8.01 um vs. 112.10±5.44 um, p<0.01) in inner retina thickness than HWD. The course of disease influenced the retina thickness, male patients had thinner inner retina thickness than female patients.
Conclusion: Our results demonstrated that WD had thinner inner retina thickness than HC and NWD had thinner inner retina thickness than HWD. We spectualted the thickness of inner retina layer may a potential useful biomarker for NWD.
Inequalities in access to NHS primary care dental services in Scotland during the COVID-19 pandemic
Authors: Aminu Abodunrin , McMahon Alex , Clark Claire , Sherriff Andrea , Buchanan Caroline , Watling Chris , Mahmoud Ahmed , Culshaw Shauna , MacKay William , Gorman Megan , Braid Raymond , Edwards Maura , Conway David 
Published: November 2022

Abstract
Introduction: This study aims to quantify the impact of the COVID-19 pandemic on access and inequalities in primary care dental services among children and adults in Scotland.
Methods: Access was measured as any NHS Scotland primary care dental contacts derived from administrative data from January 2019 to May 2022, linked to the area-based Scottish Index of Multiple Deprivation (SIMD) for children and adults, and related to population denominator estimates from National Record Scotland. Inequalities for pre-pandemic (January 2019 – January 2020) and recent (December 2021 – February 2022 and March 2022 – May 2022) periods for both children and adults were calculated and compared using the slope index of inequality (SII) and relative index of inequality (RII).
Results: Following the first lockdown (March 2020) there was a dramatic fall to near zero dental contacts, followed by a slow recovery to 64.8% of pre-pandemic levels by May 2022. There was initial widening of relative inequalities in dental contacts in early 2022, which, more recently, had begun to return to pre-pandemic levels.
Conclusion: COVID-19 has had a major impact on access to NHS primary dental care, and while inequalities in access are apparent as services recover from lockdown, these inequalities are not a new phenomenon.
Changing Patterns in temporal trends of diabetes mortality in China from 1987-2020: a rapid growth among rural populations
Authors: chen chen , Su Binbin , Zhao Yihao , Wu Yu , Zhong Pangliang , Zheng Xiaoying 
Published: November 2022

Abstract
Diabetes mellitus (DM) is a public health challenge in China. Quantifying changes in DM mortality is of great significance, but related evidence of Chinese older adults is scarce. This study conducted a comprehensive analysis of China’s DM mortality changing trajectory. The data from 1987 to 2020 was derived from the National Health Commission of China. Joinpoint analysis and age-period-cohort (APC) model were used to calculate the annual change and the effects of age, period and cohort on DM mortality. The changing trajectory from 1987 to 2020 showed an upward trend, especially among rural groups, and the gap between rural-urban narrowed. Joinpoint analysis showed a consistent upward trend in rural areas (4.9%, 95%CI: 4.0%-5.7%). However, the urban showed an upward trend from 1987–1998 (5.7%) and a slow downward trend from 1998 to 2020 (-1.0%). The APC model showed that the reporting rate increased with age in urban and rural areas. For the effect of time period and cohort, as they proceeded, the rural showed a consistent and significant upward trend, but the trends in urban remained relatively stable. The DM mortality in China has shown an overall upward trend over the past three decades, especially in rural areas. Although the gap between urban-rural has been further reduced, the burden of the oldest-older has risen significantly regardless of areas. It is time to give priority attention to the high mortality of DM among females, the elderly and rural areas, and the government should take measures to curb this trend.
Anaphylactic Shock Following Spray of “Gulliver” (Azimsulfuron), A Herbicide Belong To Sulfonylurea Group; A Case Report
Authors: Lankarathna Aluth Patabedige Chamila Pradeep , Medagama Arjuna 
Published: November 2022

Abstract
Background
Anaphylactic shock is a life threatening emergency encountered frequently with rising incidence worldwide. It is an extreme end of a spectrum of allergic reactions commonly present following multiple triggers, including food, medications, insect stings, dyes, etc. Patients usually present with a combination of dermatological, cardiovascular, respiratory and gastrointestinal symptoms. This is a rarely encountered case of anaphylactic shock following the spraying of sulphur containing herbicide called “Gulliver” (Azimsulfuron).
Case presentation
This 58-year-old farmer with previously uneventful past medical history was admitted to the emergency unit with an episode of faintishness and urticarial rash while working in a paddy field. There was no shortness of breath, wheezing or angioedema. He revealed that he had sprayed an herbicide call ‘Gulliver’ (Azimsulfuron, a sulphur containing herbicide) in the morning for 3 to 4 hours without using proper protective equipments and cloths. He denied any insect bites or stings. On admission, he was in shock with blood pressure 60/48mmHg, pulse rate 84 beats per minute with bounding pulses and warm peripheries. He was managed as anaphylactic shock according to the national health guideline and hemodynemically stabilized within 20 minutes of initial resuscitation including intramuscular adrenaline.
Conclusions
It is a well-known fact that a significant proportion of the general population has an allergy to sulphur containing medications. However, this was a rare encounter, detected with a high degree of suspicion. In Sri Lanka, a survey in 2016/2017 revealed 27 percent of the total labour force was engaged in the agricultural sector. We emphasize the importance of consideration of allergy and anaphylaxis in similar presentation in an agricultural based country like Sri Lanka.
Biochemical responses in cotton as diagnostic parameter for resistance against cotton leaf curl virus (CLCuV)
Authors: Chauhan Prashant , Mehta Naresh , Chauhan R. S. , Kumar Abhishek , Singh Harbinder 
Published: November 2022

Abstract
Cotton (Gossypium hirsutum L) is one of the most important staple fibrous crops cultivated in India and globally. Its production and quality are greatly hampered by cotton leaf curl disease (CLCuD) caused by cotton leaf curl virus (CLCuV). Therefore, the aim of present study was to investigate biochemical resistance responses in different cotton varieties against CLCuV. Four commercial cotton varieties with susceptible (HS 6 and RCH-134 BG-II) and resistant (HS 1236 and Bunty) response were used to analyse the role of primary (sugar, protein and chlorophyll) and secondary (gossypol, phenol and tannin) biochemical compounds produced by the plants against infection of CLCuV. The resistant cultivars with increased activity of protein, phenol and tannin exhibited as biochemical barriers against CLCuV infection imparting the resistance in cotton cultivars. Whereas, other biochemical compounds including chlorophyll, sugar and gossypol did not show significant role in resistance against CLCuV. Nevertheless, these compounds virtually associated with basic physiological and metabolic mechanisms of cotton plants. Among the primary biochemical compounds, only protein activity proposed as first line of defence in cotton against CLCuV. The secondary level of defence line in resistance exhibited the activity of secondary biochemical compounds phenol and tannins which exhibited significant increase in their level while imparting resistance against CLCuV in cotton.
Assessing Secular Trends in HIV Rapid Diagnostic Test Uptake and Positivity in Northeast Iran, a country in MENA region, and Recommending Gender-Specific Health Policies to Improve.
Authors: Yousefli Zahra , Maharlouei Najmeh , Moghaddam Maliheh Dadgar , Hosseinpour Ali Mohammad , Ghiami Roohollah 
Published: November 2022

Abstract
Background
Iran is among the first three countries in MENA region where two-thirds of new HIV infections are reported. The HIV rapid diagnostic testing (HIV-RDT) program, established for early detection of HIV infection, is evaluated in Mashhad, a metropolitan area, and 14 cities in Khorasan-Razavi Province, Iran.
Methods
In this cross-sectional study, de-identified records of HIV-RDT clients between 2017 and 2021 were extracted from the electronic health information system of 122 testing sites. Descriptive, bivariate and multivariate logistic regression analyses were performed to identify the factors associated with HIV-RDT uptake and risks and drivers of HIV-RDT positivity.
Results
Conducting 66546 HIV-RDTs among clients with a mean age of 30.31 years, 63% female, 75.2% married, and 78.5% diploma or below, yielded 312 (0.47%) positive results. Prenatal care and suspicious heterosexual intercourse were the most frequent reasons for taking HIV-RDT among women and men, respectively (76% and 61.2%). Test uptake was relatively low among men and the unmarried population. Male gender (odd ratio (OR) = 9.5), higher age at the time of testing (OR = 1.03), widowhood (OR = 3.98), education levels of high school diploma or below (OR = 2.35), and unemployment status (OR = 2.91) were significant sociodemographic predictors of positive HIV-RDT (P-value < 0.05). Furthermore, gender-stratified multivariate analyses revealed unsanitary dental procedures (OR = 15.7), mother-to-child transmission (MTCT)(OR = 29.95), drug injection (OR = 8.21), and male homosexuality (OR = 19.38) as behavioral predictors of test positivity among males (P-value < 0.05). Whilst substance abuse (not only injection) (OR = 12.15), MTCT (OR = 13.54), and being an occupational trainee (OR = 15.08) were predictors among females (P-value < 0.05).
Conclusion
The study provides significant targets to implement innovative strategies to increase test uptake and yield in the region, and also suggests gender-specific strategies, according to the sociodemographic and behavioral risks, for HIV primary prevention and early detection.
Role of toll-like receptors on placental derived mesenchymal stem cells and their protection from natural killer cells
Authors: Chen Gecai , Wang Meixiang , Yue Aihuan , Ruan Zhongbao , Zhu Li , Tang Chengchun 
Published: November 2022

Abstract
NK cells are major effector cells of the innate immunity. It is not clear that how MSCs can escape allogeneic NK cells killing and survival. The study included human placenta from women who were healthy and had full-term naturally-delivered male newborns (n = 5). Peripheral blood samples were obtained from healthy voluntary donors (n = 5). Karyotype analysis was carried out to determine the origin of the MSCs. Expression of TLR1-TLR10 was assessed by PCR. IL-1beta, IL-6 and IL-8 in MSCs were primed by Toll-like receptor agonists. MSCs primed by different Toll-like receptor (TLR) agonists and NK cells activated with IL-2 were co-cultured. CD107α were analyzed by flow cytometry. The NK cell-activating receptors NKp30, NKG2D, and DNAM-1 were analyzed by FCM. Also the levels of IL-10, IFN-γ and TNF-αwere evaluated by ELISA. Karyotype analyses indicated that cells were derived from the maternal matrix. The expression of TLR3 and 4 was higher when compared to the other molecules. In CD107α degranulation assay, MSCs primed by poly (I: C) and LPS reduced cytotoxic granules with significant differences (P < 0.05) when compared to the positive group. The NK cell-activating receptors NKp30, NKG2D, and DNAM-1 were high expression in activated NK group, but were inhibited during co-culture with MSC and primed MSC. The higher levels of IFN-γ, IL-10 and TNF-αwhich augment MSC suppressive function were secreted in B,C and D group. TLR3 and 4-primed MSCs reduced cytotoxicity of NK cells and protected from NK cell killing.
Mindfulness-Based Stress Reduction (MBSR) Effects on the Worries of Women with Poly Cystic Ovary Syndrome (PCOS)
Authors: Salajegheh Zahra , Ahmadi Atefeh , Shahrahmani Hadis , Jahani Yunes , Alidousti Katayoun , Amiri Fatemeh Nasiri , Salari Zohreh 
Published: November 2022

Abstract
Introduction

Polycystic ovary syndrome is one of the most common endocrine disorders in women that can cause anxiety, psychological distress and reduced quality of life. Therefore, the present study aimed to determine the effect of mindfulness-based stress reduction counseling on the worries of women with polycystic ovary syndrome.

Materials and methods

This quasi experimental was implemented on 60 women with polycystic ovary syndrome, referring to health centers in Kerman, Iran, from April to September 2021. In the intervention group, MBSR was conducted in eight 90-minute sessions twice a week. A researcher-made questionnaire with 34 questions (with six domains includes worries related to mental complications, interpersonal problems, non-pregnancy physical complications, pregnancy complications, sexual complications, and religion issues) on the worries of women with polycystic ovary syndrome were completed by the participants in two intervention and control groups as pre and posttest and one month later. 22 SPSS statistical software was used for analysis.

Results

The mean score of worries in the intervention group(48.18 ± 5.18) compared to the control group(75.73 ± 8.08) was significantly reduced in total and in all six domains immediately after the intervention (P < 0.0001). One month later also the total mean score of worries and subtitles decreased significantly(P < 0.0001), in intervention group (38.27 ± 3.58) in comparision with control group(76.13 ± 7.52).

Conclusion

Results showed that, the method of reducing stress based on mindfulness has caused a significant reduction in worries in the intervention group. Therefore, this method can be used to improve the mental health of this group of patients in health centers.
ArduHydro: A Low-Cost Device for Water Level Measurement and Monitoring
Authors: Galli Andrea , Peruzzi Cosimo , Gangi Fabiola , Masseroni Daniele 
Published: November 2022

Abstract
ArduHydro is a low-cost device for water level measurement and monitoring designed for a short and long-term employment in controlled and outdoor environments. It measures water level through an ultrasonic sensor and elaborates the signals through an Arduino micro controller. The small size of this device, the low energy required for its operation, its robustness and accuracy make ArduHydro properly versatile for different applications in the field for water control and management. This article describes the design, the components, the costs, and the performance of ArduHydro. Performance was assessed with a laboratory test inside a hydraulic circuit constituted by an open channel flume and comparing ArduHydro measurements with those obtained with a traditional ultrasonic sensor. An example of ArduHydro application for detecting the wavefront evolution during a surface irrigation is presented as well. The results revealed that ArduHydro measurements were on average very consistent with those obtained by the traditional ultrasonic sensor in all different flow conditions (i.e. different flowrate and water depth) demonstrating its reliability and accuracy in the measuring water level. The application of ArduHydro during a surface watering of an agricultural field allowed to obtain important spatio-temporal information about the water depth along the longitudinal direction of the field, paying the way for a real comprehension of the dynamics of wavefront evolution in a real-world case study.
A Linear Heuristic for Multiple Importance Sampling
Authors: Sbert Mateu , Szirmay-Kalos László 
Published: November 2022

Abstract
Multiple Importance Sampling (MIS) combines the probability density functions (pdf) of several sampling techniques into an importance function. The combination weights are the proportion of samples used for the particular techniques. This paper addresses the determination of the optimal combination weights from a few initial samples. Instead of the numerically unstable optimization of the variance, in our solution the quasi-optimal weights are obtained by solving a linear equation, which leads to simpler computations and more robust estimations. The proposed method is validated with 1D numerical examples and with the direct lighting problem of computer graphics.
AMS Subject Classification: Primary Computer Graphics G.3 Mathematics of Computing / PROBABILITY AND STATISTICSProbabilistic algorithms; Computer Graphics G.3 Mathematics of Computing / PROBABILITY AND STATISTICS Probabilistic algorithms ; secondary Computer Graphics G.3 Mathematics of Computing / PROBABILITY AND STATISTICS Probabilistic algorithms
Gut microbiome composition in Lynch syndrome with and without history of colorectal neoplasia and non-Lynch controls
Authors: Rifkin Samara B , Sze Marc A. , Tuck Kirsten , Koeppe Erika , Rifkin Elena M. Stoffel , Schloss Patrick D 
Published: November 2022

Abstract
Background
While Lynch syndrome (LS) is a highly penetrant colorectal cancer (CRC) syndrome, there is considerable variation in penetrance, few studies have investigated the association between microbiome and CRC risk in LS. We analyzed the microbiome composition among individuals with LS with and without personal history of colorectal neoplasia (CRN) and non-LS controls.
Methods
We sequenced the V4 region of the 16S rRNA gene from the stool of 46 individuals with LS and 53 individuals without LS. We characterized within community and in between community microbiome variation, compared taxon abundance and built machine learning models to investigate differences in microbiome.
Results
There was no difference within or between community variation among LS groups, but there was a statistically significant difference in both within and between community variation comparing LS to non-LS. Streptococcus and Actinomyces were differentially enriched in LS-CRC compared to LS-without CRN. There were numerous differences in taxa abundance comparing LS to non-LS notably Veillonella was enriched and Facaelibacterium and Romboustia were depleted in LS. Finally, machine learning models classifying LS from non-LS controls and LS-CRC from LS-without CRN performed moderately well.
Conclusions
Differences in microbiome composition between LS and non-LS may suggest a microbiome pattern unique to LS formed by underlying differences in epithelial biology and immunology. We found specific taxa differences among LS groups, which may be due to underlying anatomy. Larger prospective studies following for CRN diagnosis and microbiome composition changes are needed to determine if microbiome composition contributes to CRN development in patients with LS.