bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensitive and Selective Analytical Method for the Quantification of Two Potential Genotoxic Impurities in Azilsartan Drug Substance by using LC-MS/MS with Multiple Reaction Monitoring (MRM mode)
Authors: Alluri Chandramohan , Naramsetti Kiran Kumar , Sharma G. Veera Raghava 
Published: April 2022

The main aim of the present study to Synthesize,method development and method validation for quantification of two potential genotoxic impurities i.e., Methyl(Z)-2-ethoxy-1-((2'-(N'-hydroxycarbamimidoyl)-[1,1'-biphenyl]-4-yl) methyl)-1H-benzo[d]imidazole-7-carboxylate(Impurity-A) and 2-ethoxy-1-((2'-(N'-hydroxycarbamimidoyl[)-[1,1'-biphenyl]-4-yl)methyl)-1H-benzo[d]imidazole-7-carboxylicacid(Impurity-B) by using LC-MS/MS MRM mode at trace level determination inAzilsartan drug substance. The new LC-MS/MS MRM mode method was developed by using Inertsil ODS-3V 150x4.6mm,5µm column as stationary phase. The mobile phase used is the composition of 10Mm Ammonium formate pH3.00buffer:Acetonitrile(9:1)%v/v as mobilephase-A and 10Mm Ammonium formate pH3.00buffer:Acetonitrile(2:8)%v/v as mobile phase-B, isocratic elution of mobile phase-A and Mobile phase-B (60:40)v/v at flow rate of 0.8mL/min.The concentration limits of the both genotoxic impurities were calculated a limit of 37.5ppm based on the concept of TTC (threshold of toxicological concern) and MDD (maximum daily dosage which is 40mg/day for Azilsartan drug substance.The limit of detection(LOD) was found to be 1.4ppm for both Impurity-A and Impurity-B.The limit of quantification(LOQ) for Impurity-A was 4.7ppm and Impurity-B was 4.5ppm respectively The method was found to be linear from 4.7ppm to 78.4ppm for Impurity-A (correlation coefficient:1.000) and 4.5ppm to 75.7ppm for Impurity-B (correlation coefficient:0.999). The method was precise and found percentage of relative standard deviation for six replicate sample preparations of Impurity-A and Impurity-B was below 5.0%. The method accuracy was confirmed based on the recovery studies. Based on the method validation study method was sensitive and selective for quantification both genotoxic impurities.
Isolation and Enzymatic Activity of Thermo-tolerant Bacteria from Waste Dumpsites in Umudikeand Environs
Authors: Obi C. N. , Ihuoma B. O. , Ike S. 
Published: April 2022

The research studied the isolation of thermo-tolerant bacteria with enzymatic activity from waste dumpsites. 30 soil samples were collected using sterile spatular into sterile universal bottles, labelled and taken to the laboratory analyses. The soil samples were serially diluted and inoculated by spread plate method on different media and incubated at 30oC for 48 hrs. The isolates were observed for colonial morphologies and later sub-cultured to get pure isolates which were stored in agar slants and kept in the refrigerator for further use. The isolates were Gram stained and also subjected to biochemical and sugar fermentation tests. The isolates were then cultured at thermophilic temperature and those with positive results were screened for the production of amylase, protease and lipase activity. The effect of temperature, pH and nitrogen sources on enzyme activity of the thermophilic bacterial isolates was also assessed. Results show that Bacillus subtilis (70.0%), Bacillus licheniformis (43.3%), Bacillus cereus (56.7%), Pseudomonas aeruginosa (56.7%), Streptomyces species (36.7%), Bacillus brevis (43.3%)and Norcadia species (30%) were recovered from the soil samples. All the bacterial isolates tolerated 55oC of temperature while only Bacillus cereus, Bacillus brevis and Norcadia species were isolated at 60oC. All the isolates except Pseudomonas aeruginosa gave amylase activity while all the isolates gave protease activity. All the isolates except Bacillus licheniformis and Nocardia species had lipase activity. Temperature and pH had varied effects on the enzyme activity of the isolates. The potentials of these bacterial isolates as good sources of enzymes at elevated temperatures, varied pH and nitrogenous sources for commercial uses was recognized.
Prevalence and patterns of symptoms of dysautonomia in patients with long‐COVID syndrome: A cross‐sectional study
Authors: Eldokla Ahmed M. , Mohamed‐Hussein Aliae A. , Fouad Ahmed M. , Abdelnaser Mariam G. , Ali Sara T. , Makhlouf Nahed A. , Sayed Islam G. , Makhlouf Hoda A. , Shah Jaffer , Aiash Hani 
Published: April 2022

AbstractBackgroundThe association between autonomic dysfunction and long‐COVID syndrome is established. However, the prevalence and patterns of symptoms of dysautonomia in long‐COVID syndrome in a large population are lacking.ObjectiveTo evaluate the prevalence and patterns of symptoms of dysautonomia in patients with long‐COVID syndrome.MethodsWe administered the Composite Autonomic Symptom Score 31 (COMPASS‐31) questionnaire to a sample of post‐COVID‐19 patients who were referred to post‐COVID clinic in Assiut University Hospitals, Egypt for symptoms concerning for long‐COVID syndrome. Participants were asked to complete the COMPASS‐31 questionnaire referring to the period of more than 4 weeks after acute COVID‐19.ResultsWe included 320 patients (35.92 ± 11.92 years, 73% females). The median COMPASS‐31 score was 26.29 (0–76.73). The most affected domains of dysautonomia were gastrointestinal, secretomotor, and orthostatic intolerance with 91.6%, 76.4%, and 73.6%, respectively. There was a positive correlation between COMPASS‐31 score and long‐COVID duration (p < 0.001) and a positive correlation between orthostatic intolerance domain score and post‐COVID duration (p < 0.001). There was a positive correlation between orthostatic intolerance domain score and age of participants (p = 0.004). Two hundred forty‐seven patients (76.7%) had a high score of COMPASS‐31 >16.4. Patients with COMPASS‐31 >16.4 had a longer duration of long‐COVID syndrome than those with score <16.4 (46.2 vs. 26.8 weeks, p < 0.001).ConclusionsSymptoms of dysautonomia are common in long‐COVID syndrome. The most common COMPASS‐31 affected domains of dysautonomia are gastrointestinal, secretomotor, and orthostatic intolerance. There is a positive correlation between orthostatic intolerance domain score and patients' age.
Biomass‐Derived Carbon for High‐Performance Batteries: From Structure to Properties
Authors: Sun Yu , Shi Xiao‐Lei , Yang Yan‐Ling , Suo Guoquan , Zhang Li , Lu Siyu , Chen Zhi‐Gang 
Published: April 2022

AbstractOwing to the sustainability, environmental friendliness, and structural diversity of biomass‐derived materials, extensive efforts have been devoted to use them as energy storage materials in high‐energy rechargeable batteries. A timely and comprehensive review from the structures to mechanisms will significantly widen this research field. Here, it starts with the operation mechanism of batteries, and it aims to summarize the latest advances for biomass‐derived carbon to achieve high‐energy battery materials, including activation carbon methods and the structural classification of biomass‐derived carbon materials from zero dimension, one dimension, two dimension, and three dimension. Each strategy starts with carefully selected examples and then moves to illustrate the underlying transport mechanism of electrons in the structure. In the end, challenges, strategies, and outlooks are pointed out for the future development of biomass‐derived carbon materials. Overall, this review will help researchers choose appropriate strategies to design biomass‐derived carbon materials, thereby promoting the application of biomass materials in battery design.
Metabolic Intervention Nanoparticles for Triple‐Negative Breast Cancer Therapy via Overcoming FSP1‐Mediated Ferroptosis Resistance
Authors: Yang Jie , Jia Zengguang , Zhang Jun , Pan Xiuhua , Wei Yawen , Ma Siyu , Yang Ning , Liu Zengyi , Shen Qi 
Published: April 2022

AbstractTriple‐negative breast cancer (TNBC) patients have a predisposition to poor prognosis due to the strong malignancy. Ferroptosis, a new form of cell death, is a candidate treatment for TNBC owing to its effectiveness in killing cancer cells. However, some TNBC cells exhibit an abnormal tumor metabolism, especially the ferroptosis suppressor protein 1 (FSP1)‐mediated ubiquinone redox metabolism, which can promote ferroptosis resistance. Here, rosuvastatin (RSV) is encapsulated in silk fibroin (SF) nanoparticle (designated as Cu‐SF(RSV) NPs) for TNBC inhibition by overcoming FSP1‐mediated ferroptosis resistance. RSV intervenes in metabolic mevalonate pathway to disturb the redox homeostasis regulated by CoQ/FSP1 axis, thereby overcoming ferroptosis resistance. Besides, Cu‐SF(RSV) NPs can generate reactive oxygen species and deplete glutathione to facilitate redox stress, thereby amplifying ferroptosis effect. Thus, it is anticipated that the metabolic intervention nanoparticles, Cu‐SF(RSV) NPs, can be exploited as a promising therapeutic platform for clinical TNBC treatment.
Wireless Electrical Power Delivery Using Light through Soft Skin Tissues under Misalignment and Deformation
Authors: Seo Jimin , Kim Juho , Jeong Jinmo , Jung Dongwuk , Ju Hunpyo , Lee Taeyeon , Park Hyeongoh , Lee Jongho 
Published: April 2022

AbstractProviding sustainable electrical power to medical electronic implants is key to enabling the diverse functions of implants and to avoiding repetitive replacement surgeries of depleted batteries. Subcutaneous photovoltaic energy harvesting with an external light source patch can be a reliable approach that provides sufficient power using light. However, from a practical perspective, aligning the external light source patch over opaque skin by patients may affect the amount of delivered electrical power. The amount of delivered power may also be affected by the deformation of the light source and receiver patches in accordance with thin skin layers. In this paper, an experimental and analytical study on the performance of a power implant under an animal skin depending on the implantation depth, lateral misalignment with the external light patch, and bending radius and direction is presented. The experimental results indicate that the transferred power by the patches in misalignment (about 20% of the transmitter length) or in bending (≈30 mm radius) is maintained at least 90% power of the aligned and flat ones. The results provide important characteristic data that are required in designing reliable wireless power transfer systems for various types of implantable medical devices.
Ultrawide‐Angle Ultralow‐Reflection Phenomenon for Transverse Electric Mode in Anisotropic Metasurface
Authors: Lv Qihao , Jin Cheng , Zhang Binchao , Yin Liyuan , Liu Shuo , Tian Buning , Cui Tie Jun 
Published: April 2022

AbstractThe Brewster effect plays a significant role in the transmission of electromagnetic waves with a transverse magnetic mode or p‐polarization at a specific angle. Here, a transverse electric mode ultrawide‐angle ultralow‐reflection phenomenon is proposed to realize perfect transparency at ultrawide incidence angles. Theoretical formulae are derived to provide the basis for the ultrawide‐angle ultralow‐reflection phenomenon and to explain the significant characteristics of achieving an ultrawide‐angle transmission response. As a proof of concept, a metasurface with an ultrawide‐angle ultralow‐reflection response is designed and measured, whose relative permittivity and permeability tensors are tuned by engineering an electric resonator and a magnetic resonator, respectively. This study unveils a new path for the design of ultrawide‐angle transparent surfaces.
Recent Advances in Reactions Using Enaminone in Water or Aqueous Medium
Authors: Wang Zhouying , Zhao Baoli , Liu Yunyun , Wan Jie‐Ping 
Published: April 2022

AbstractEnaminones have in the past decade received renewed interest in organic synthesis by acting as highly versatile building blocks. Particularly, the featured amino and carbonyl group in the enaminone structure endow them with the ability of tolerating water via hydrogen bonding effect. Thus, designing synthetic methods in aqueous medium with enaminones as the main building blocks has received extensive attention and notable success. Based on our longstanding efforts and interests in enaminone chemistry, we review herein the research advances in enaminone participated organic synthesis in aqueous medium.magnified image
Giant Extrinsic Spin Hall Effect in Platinum‐Titanium Oxide Nanocomposite Films
Authors: Xu Xinkai , Zhang Dainan , Liu Bo , Meng Hao , Xu Jiapeng , Zhong Zhiyong , Tang Xiaoli , Zhang Huaiwu , Jin Lichuan 
Published: April 2022

AbstractAlthough the spin Hall effect provides a pathway for efficient and fast current‐induced manipulation of magnetization, application of spin–orbit torque magnetic random access memory with low power dissipation is still limited to spin Hall materials with low spin Hall angles or very high resistivities. This work reports a group of spin Hall materials, Pt1−x(TiO2)x nanocomposites, that combines a giant spin Hall effect with a low resistivity. The spin Hall angle of Pt1−x(TiO2)x in an yttrium iron garnet/Pt1−x(TiO2)x double‐layer heterostructure is estimated from a combination of ferromagnetic resonance, spin pumping, and inverse spin Hall experiments. A giant spin Hall angle 1.607 ± 0.04 is obtained in a Pt0.94(TiO2)0.06 nanocomposite film, which is an increase by an order of magnitude compared with 0.051 ± 0.002 in pure Pt thin film under the same conditions. The great enhancement of spin Hall angle is attributed to strong side‐jump induced by TiO2 impurities. These findings provide a new nanocomposite spin Hall material combining a giant spin Hall angle, low resistivity and excellent process compatibility with semiconductors for developing highly efficiency current‐induced magnetization switching memory devices and logic devices.
Famine, tea, and bread in Ireland: C282Y and modern human microevolution
Authors: Burke Stacie , Duffy Thomas 
Published: April 2022

AbstractThe HFE gene variant allele C282Y connected with hereditary hemochromatosis occurs at a frequency of about 10%–11% in Ireland, the highest known frequency in the modern global population. In this synthesis, we draw together an interdisciplinary literature to offer an agriculturally‐grounded, biocultural example of modern human adaptation and microevolution that addresses Ireland's comparatively high C282Y frequency. We argue that changing subsistence‐, diet‐, and nutrition‐related stressors framed under colonial governance played a role shaping the biology of Ireland's population historically, maintaining the C282Y variant. A population accustomed to challenges of poverty and difficult living conditions, the Irish fell under the influence of massive ecological stress with the fungus (Phytophthora infestans), the failed potato crops, and the Great Famine of 1845–52. From there, in the post‐Famine era, diet and nutrition altered further as the potato was exchanged for gluten‐rich wheaten bread and stewed black tea. With risks for iron deficiency anemia heightened by the cultural construction of diet and niche, an adaptive iron‐conserving advantage continued to be offered by the C282Y allele, influencing survival into the post‐Famine era. More recently, however, C282Y has been reframed as a potential risk in the context of contemporary, iron fortified diets.
Membrane Cholesterol Depletion Enhances Enzymatic Activity of Cell‐Membrane‐Coated Metal‐Organic‐Framework Nanoparticles
Authors: Wang Shuyan , Kai Mingxuan , Duan Yaou , Zhou Zhidong , Fang Ronnie H. , Gao Weiwei , Zhang Liangfang 
Published: April 2022

AbstractMetal‐organic‐framework nanoparticles (MOF NPs) have been increasingly used to encapsulate therapeutic enzymes for delivery. To better interface these MOF NPs with biological systems, researchers have coated them with natural cell membranes, enabling biomimicking properties suitable for innovative biomedical applications. Herein, we report that the enzymatic activity of cell‐membrane‐coated MOF NPs can be significantly enhanced by reducing membrane cholesterol content. We demonstrate such cholesterol‐enzymatic activity correlation using zeolitic imidazolate framework‐8 MOF NPs to encapsulate catalase, horseradish peroxidase, and organophosphate hydrolase, respectively. MOF NPs coated with membranes of human red blood cells or macrophages show similar outcomes, illustrating the broad applicability of this finding. The mechanistic investigation further reveals that reducing cholesterol levels effectively enhances membrane permeability likely responsible for the increased enzymatic activity. These results also imply a facile approach to tailoring the enzymatic activity of cell‐membrane‐coated MOF NPs by simply tuning the membrane cholesterol level.
Oxygen Vacancy Management for High‐Temperature Mesoporous SnO2 Electron Transport Layers in Printable Perovskite Solar Cells
Authors: Liu Jiale , Li Sheng , Liu Shuang , Chu Yanmeng , Ye Ting , Qiu Cheng , Qiu Zexiong , Wang Xiadong , Wang Yifan , Su Yaqiong , Hu Yue , Rong Yaoguang , Mei Anyi , Han Hongwei 
Published: April 2022

AbstractThe planar SnO2 electron transport layer (ETL) has contributed to the reported power conversion efficiency (PCE) record of perovskite solar cells (PSCs), while the high‐temperature mesoporous SnO2 ETL (mp‐SnO2) brings poor device performance. Herein, we report the application of mp‐SnO2 for efficient printable PSCs via oxygen vacancy (OV) management by introducing magnesium (Mg) into the paste. We find that high‐temperature annealing suppresses self‐doping of SnO2 by reducing OVs. The introduced Mg occupies both the Sn site and interstitial site of SnO2 and promotes the formation of OVs. Lattice Mg tends to induce neutral OVs and interstitial Mg could promote the ionization of neutral OVs for self‐doping. The synergy effect on OVs increases the carrier density and upshifts the Fermi level energy of mp‐SnO2, ensuring its capability as the well‐performed ETL with trap‐less charge transport and suppressed surface recombination for dramatic improved device PCE from 6.62 % to 17.25 %.
Catalytic Synthesis of Formamides by Integrating CO2 Capture and Morpholine Formylation on Supported Iridium Catalyst
Authors: Cheng Danyang , Wang Meng , Tang Lipeng , Gao Zirui , Qin Xuetao , Gao Yongjun , Xiao Dequan , Zhou Wu , Ma Ding 
Published: April 2022

AbstractHerein we report an efficient and recyclable catalytic system for tandem CO2 capture and N‐formylation to value‐added chemicals. CO2 is apt to be captured by morpholine solution, while a highly efficient heterogeneous catalyst, isolated iridium atoms supported over nanadiamond/graphene, is discovered to be highly reactive for the formylation of morpholine, leading to the formation of N‐formylmorpholine with excellent productivity (with a turnover number of 5 120 000 in a single batch reaction) and selectivity (>99 %). In addition, the CO2 captured by morpholine under atmospheric conditions can be converted to N‐formylmorpholine with decent conversion (51 %), which realizes the integration of CO2 capture and conversion to value‐added chemicals.
Design, synthesis, cytotoxicity, and molecular docking studies of novel thiazolyl–hydrazone derivatives as histone lysine acetyl‐transferase inhibitors and apoptosis inducers
Authors: Abbas Eman M. H. , Farghaly Thoraya A. , Sabour Rehab , Shaaban Mohamed R. , Abdallah Zeinab A. 
Published: April 2022

AbstractCompounds containing both thiazole and arylsulfone moieties are recognized for their high biological activity and ability to fight a variety of ailments. Thus, in this context, new derivatives of (thiazol‐2‐yl)hydrazone with an arylsulfone moiety were synthesized as CPTH2 analogs with potent anti‐histone lysine acetyl‐transferase activity. Compounds 3, 4, 10b, and 11b showed an excellent inhibitory effect on P300 (E1A‐associated protein p300), compared to CPTH2. Among all the tested derivatives, compound 10b revealed the highest activity against both P300 and pCAF. In addition, the new hits were tested for anticancer efficacy against two leukemia cell lines. Most of them showed a moderate to potent antitumor effect on the k562 and CCRF‐CEM cell lines. Interestingly, the activity of compound 10b against the k562 cell line was found to be higher than that of CPTH2. Furthermore, it showed a good safety profile, better than CPTH2 on normal cells. Molecular docking analysis was carried out to reveal the crucial binding contacts in the inhibition of the P300 and pCAF enzymes.
Density functional theory study on the reductive elimination of ancillary ligand at PPC nickel complexes
Authors: Kim Yongseong , Cho Hyun , Hwang Sungu 
Published: April 2022

AbstractDensity functional theory calculations were performed to investigate the reactions between an alkyl (or aryl) substrate and a pincer ligand at the κ3‐PPC Ni center, where reductive elimination or H elimination reactions occur. Ni complexes with PPC‐type pincer ligand showed different reactivity toward substrate, unlike conventional PCP‐type ligands. For substrates lacking β‐hydrogen, reductive elimination is preferred over completing α‐hydrogen elimination. For ethyl substrate, an intermediate and a TS for β‐H transfer with Gibbs free energy comparable to the reductive elimination were located.
Molecular landscape of TP53 mutations in breast cancer and their utility for predicting the response to HER‐targeted therapy in HER2 amplification‐positive and HER2 mutation‐positive amplification‐negative patients
Authors: Liu Binliang , Yi Zongbi , Guan Yanfang , Ouyang Quchang , Li Chunxiao , Guan Xiuwen , Lv Dan , Li Lixi , Zhai Jingtong , Qian Haili , Xu Binghe , Ma Fei , Zeng Yixin 
Published: April 2022

AbstractPurposeWe used targeted capture sequencing to analyze TP53‐mutated circulating tumor DNA (ctDNA) in metastatic breast cancer patients and to determine whether TP53 mutation has predictive value for anti‐human epidermal growth factor receptor 2 (HER2) treatment for in HER2 amplification‐positive patients (HER2+) and HER2 mutation‐positive, amplification‐negative (HER2−/mut) patients.Patients and MethodsTP53 mutation features were analyzed in the Geneplus cohort (n = 1184). The MSK‐BREAST cohort was used to explore the value of TP53 mutation in predicting anti‐HER‐2 antibody efficacy. Sequencing of ctDNA in phase Ib, phase Ic, phase II clinical trials of pyrotinib (HER2+ patients), and an investigator‐initiated phase II study of pyrotinib (HER2−/mut patients) were performed to analyze the relationships between TP53 mutation and prognosis for HER2 TKIs. The MSK‐BREAST cohort, MutHER, and SUMMIT cohort were used for verification.ResultsTP53 mutations were detected in 53.1% (629/1184) of patients in the Geneplus cohort. The TP53 mutation rate was higher in HR‐negative (p < 0.001) and HER2 amplification‐positive (p = 0.015) patients. Among patients receiving anti‐HER2 antibody therapy, those whose tumors carried TP53 mutations had a shorter PFS (p = 0.004). However, the value of TP53 mutation in predicting HER2 TKI response was inconsistent. In HER2+ patients, no difference in PFS was observed among patients with different TP53 statuses in the combined analysis of the pyrotinib phase Ib, phase Ic, and phase II clinical trials (p = 1.00) or in the MSK‐BREAST cohort (p = 0.62). In HER2−/mut patients, TP53 mutation‐positive patients exhibited a trend toward worse prognosis with anti‐HER2 TKI treatment than TP53‐wild‐type patients in our investigator‐initiated phase II study (p = 0.15), and this trend was confirmed in the combined analysis of the MutHER and SUMMIT cohorts (p = 0.01).ConclusionsTP53 mutation can be used to identify biomarkers of anti‐HER2 antibody drug resistance in HER2+ patients and HER2 TKI resistance in HER2−/mut patients.