bluuebunny/crossref_metadata_2025_split huggingface dataset:
Network of the transcriptome and metabolomics reveals a novel regulation of drought resistance during germination in wheat
Authors: Li Zongzhen , Lian Yanhao , Gong Pu , Song Linhu , Hu Junjie , Pang Haifang , Ren Yongzhe , Xin Zeyu , Wang Zhiqiang , Lin Tongbao 
Published: August 2022

AbstractBackground and AimsThe North China Plain, the highest winter-wheat-producing region of China, is seriously threatened by drought. Traditional irrigation wastes a significant amount of water during the sowing season. Therefore, it is necessary to study the drought resistance of wheat during germination to maintain agricultural ecological security. From several main cultivars in the North China Plain, we screened the drought-resistant cultivar JM47 and drought-sensitive cultivar AK58 during germination using the polyethylene glycol (PEG) drought simulation method. An integrated analysis of the transcriptome and metabolomics was performed to understand the regulatory networks related to drought resistance in wheat germination and verify key regulatory genes.MethodsTranscriptional and metabolic changes were investigated using statistical analyses and gene–metabolite correlation networks. Transcript and metabolite profiles were obtained through high-throughput RNA-sequencing data analysis and ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry, respectively.Key ResultsA total of 8083 and 2911 differentially expressed genes (DEGs) and 173 and 148 differential metabolites were identified in AK58 and JM47, respectively, under drought stress. According to the integrated analysis results, mammalian target of rapamycin (mTOR) signalling was prominently enriched in JM47. A decrease in α-linolenic acid content was consistent with the performance of DEGs involved in jasmonic acid biosynthesis in the two cultivars under drought stress. Abscisic acid (ABA) content decreased more in JM47 than in AK58, and linoleic acid content decreased in AK58 but increased in JM47. α-Tocotrienol was upregulated and strongly correlated with α-linolenic acid metabolism.ConclusionsThe DEGs that participated in the mTOR and α-linolenic acid metabolism pathways were considered candidate DEGs related to drought resistance and the key metabolites α-tocotrienol, linoleic acid and l-leucine, which could trigger a comprehensive and systemic effect on drought resistance during germination by activating mTOR–ABA signalling and the interaction of various hormones.
One-year functional outcomes of patients aged 80 years or more undergoing colonic cancer surgery: prospective, multicentre observational study
Authors: Niemeläinen Susanna , Huhtala Heini , Jämsen Esa , Kössi Jyrki , Andersen Jan , Ehrlich Anu , Haukijärvi Eija , Koikkalainen Suvi , Koskensalo Selja , Mattila Anne , Pinta Tarja , Uotila-Nieminen Mirjami , Vihervaara Hanna , Hyöty Marja 
Published: August 2022

Abstract

Background
Older patients are at high risk of experiencing delayed functional recovery after surgical treatment. This study aimed to identify factors that predict changes in the level of support for activities of daily living and mobility 1 year after colonic cancer surgery.

Methods
This was a multicentre, observational study conforming to STROBE guidelines. The prospective data included pre-and postoperative mobility and need for support in daily activities, co-morbidities, onco-geriatric screening tool (G8), clinical frailty scale (CFS), operative data, and postoperative surgical outcomes.

Results
A total of 167 patients aged 80 years or more with colonic cancer were recruited. After surgery, 30 per cent and 22 per cent of all patients had increased need for support and decreased motility. Multivariableanalysis with all patients demonstrated that preoperative support in daily activities outside the home (OR 3.23, 95 per cent c.i. 1.06 to 9.80, P = 0.039) was associated with an increased support at follow-up. A history of cognitive impairment (3.15, 1.06 to 9.34, P = 0.038) haemoglobin less than 120 g/l (7.48, 1.97 to 28.4, P = 0.003) and discharge to other medical facilities (4.72, 1.39 to 16.0, P = 0.013) were independently associated with declined mobility. With functionally independent patients, haemoglobin less than 120 g/l (8.31, 1.76 to 39.2, P = 0.008) and discharge to other medical facilities (4.38, 1.20 to 16.0, P = 0.026) were associated with declined mobility.

Conclusion
Increased need for support before surgery, cognitive impairment, preoperative anaemia, and discharge to other medical facilities predicts an increased need for support or declined mobility 1 year after colonic cancer surgery. Preoperative assessment and optimization should focus on anaemia correction, nutritional status, and mobility with detailed rehabilitation plan.
Nudging for Social Change: Promises and Cautions for Social Workers to Apply Behavioural Economic Tools
Authors: Wang Julia Shu-Huah 
Published: August 2022

AbstractEmerging knowledge from the behavioural economics field has the potential to expand social workers’ repertoire of tools to facilitate clients’ behavioural change and enhance policy design. However, social work training and practice do not traditionally incorporate this school of knowledge. As an initial step to bridge social work and behavioural economics, this article introduces behavioural economic concepts to social workers. In particular, it (i) reviews behavioural tendencies that are irrational, (ii) introduces seven behavioural intervention tools (commitment devices, defaults, incentives, social comparison, planning, messaging, and simplification and salience) to address these irrationalities, (iii) discusses cautions when applying these tools, (iv) introduces potential applications in micro and macro social work practice, (v) outlines steps to incorporate behavioural economic concepts into programme/policy design and (vi) addresses ethical considerations. Enhancing social workers’ knowledge of behavioural economics can facilitate interdisciplinary communication, collaboration and cross-fertilisation. Whilst social work practice may benefit from incorporating behavioural intervention tools, social workers’ rich practice wisdom can contribute to advancing ways through which nudge interventions further facilitate cognitive changes to bring about sustainable behavioural changes.
Brain areas associated with visual spatial attention display topographic organization during auditory spatial attention
Authors: Popov Tzvetan , Gips Bart , Weisz Nathan , Jensen Ole 
Published: August 2022

AbstractSpatially selective modulation of alpha power (8–14 Hz) is a robust finding in electrophysiological studies of visual attention, and has been recently generalized to auditory spatial attention. This modulation pattern is interpreted as reflecting a top-down mechanism for suppressing distracting input from unattended directions of sound origin. The present study on auditory spatial attention extends this interpretation by demonstrating that alpha power modulation is closely linked to oculomotor action. We designed an auditory paradigm in which participants were required to attend to upcoming sounds from one of 24 loudspeakers arranged in a circular array around the head. Maintaining the location of an auditory cue was associated with a topographically modulated distribution of posterior alpha power resembling the findings known from visual attention. Multivariate analyses allowed the prediction of the sound location in the horizontal plane. Importantly, this prediction was also possible, when derived from signals capturing saccadic activity. A control experiment on auditory spatial attention confirmed that, in absence of any visual/auditory input, lateralization of alpha power is linked to the lateralized direction of gaze. Attending to an auditory target engages oculomotor and visual cortical areas in a topographic manner akin to the retinotopic organization associated with visual attention.
From urges to tics in children with Tourette syndrome: associations with supplementary motor area GABA and right motor cortex physiology
Authors: Larsh Travis R , Huddleston David A , Horn Paul S , Wu Steve W , Cecil Kim M , Jackson Hannah S , Edden Richard A E , Mostofsky Stewart H , Gilbert Donald L 
Published: August 2022

AbstractTourette syndrome (TS) is a childhood-onset disorder in which tics are often preceded by premonitory sensory urges. More severe urges correlate with worse tics and can render behavioral therapies less effective. The supplementary motor area (SMA) is a prefrontal region believed to influence tic performance. To determine whether cortical physiological properties correlate with urges and tics, we evaluated, in 8–12-year-old right-handed TS children (n = 17), correlations of urge and tic severity scores and compared both to cortical excitability (CE) and short- and long-interval cortical inhibition (SICI and LICI) in both left and right M1. We also modeled these M1 transcranial magnetic stimulation measures with SMA gamma-amino butyric acid (GABA) levels in TS and typically developing control children (n = 16). Urge intensity correlated strongly with tic scores. More severe urges correlated with lower CE and less LICI in both right and left M1. Unexpectedly, in right M1, lower CE and less LICI correlated with less severe tics. We found that SMA GABA modulation of right, but not left, M1 CE and LICI differed in TS. We conclude that in young children with TS, lower right M1 CE and LICI, modulated by SMA GABA, may reflect compensatory mechanisms to diminish tics in response to premonitory urges.
Accuracy of wristwatch-type photoplethysmography in detecting atrial fibrillation in daily life
Authors: Nonoguchi Noriko Matsushita , Soejima Kyoko , Goda Ayumi , Nishimura Kunihiro , Onozuka Daisuke , Fujita Shin , Koyama Fumio , Takano Yuichi , Iguchi Shiho , Sato Hideki , Mohri Takato , Katusme Yumi , Tashiro Mika , Hoshida Kyoko , Miwa Yosuke , Togashi Ikuko , Ueda Akiko , Sato Toshiaki , Kohno Takashi 
Published: August 2022

Abstract

Aims
Detection of asymptomatic paroxysmal atrial fibrillation is challenging. Smartphone- or smartwatch-based photoplethysmography is efficient at detecting irregular rhythms using pulse waves but is too complex for older patients. We aimed to evaluate the detection accuracy of atrial fibrillation by a wristwatch-type continuous pulse wave monitor (PWM) in daily life.

Methods and results
Patients at high risk of atrial fibrillation but with no history of atrial fibrillation (n = 163; mean CHADS2 score, 1.9) and patients with known atrial fibrillation (n = 123, including 34 with persistent atrial fibrillation) underwent PWM and telemetry electrocardiogram recording for 3 days. Risk of atrial fibrillation was judged using the ‘Kyorin Atrial Fibrillation Risk Score’, a scoring system based on previously reported atrial fibrillation risk scoring systems. The PWM assessed the presence of atrial fibrillation at 30 min intervals, and the results were compared with the telemetry electrocardiogram findings. The PWMs accurately diagnosed two patients with paroxysmal atrial fibrillation in the high-risk group. The PWMs accurately diagnosed 48 of the 55 patients with atrial fibrillation in the known-atrial fibrillation group. The PWM accuracy in detecting patients with atrial fibrillation was as follows: sensitivity, 98.0%; specificity, 90.6%; positive predictive value, 69.4%; negative predictive value, 99.5%. The respective values for intervals with atrial fibrillation were 86.9%, 98.8%, 89.6%, and 98.5%.

Conclusion
The wristwatch-type PWM has shown feasibility in detecting atrial fibrillation in daily life and showed the possibility of being used as a screening tool.
Genome assembly and annotation of the European earwig Forficula auricularia (subspecies B)
Authors: Bhattarai Upendra R , Katuwal Mandira , Poulin Robert , Gemmell Neil J , Dowle Eddy 
Published: August 2022

Abstract
The European earwig Forficula auricularia is an important model for studies of maternal care, sexual selection, sociality, and host–parasite interactions. However, detailed genetic investigations of this species are hindered by a lack of genomic resources. Here, we present a high-quality hybrid genome assembly for Forficula auricularia using Nanopore long-reads and 10× linked-reads. The final assembly is 1.06 Gb in length with 31.03% GC content. It consists of 919 scaffolds with an N50 of 12.55 Mb. Half of the genome is present in only 20 scaffolds. Benchmarking Universal Single-Copy Orthologs scores are ∼90% from 3 sets of single-copy orthologs (eukaryotic, insect, and arthropod). The total repeat elements in the genome are 64.62%. The MAKER2 pipeline annotated 12,876 protein-coding genes and 21,031 mRNAs. Phylogenetic analysis revealed the assembled genome as that of species B, one of the 2 known genetic subspecies of Forficula auricularia. The genome assembly, annotation, and associated resources will be of high value to a large and diverse group of researchers working on dermapterans.
Characterization of Plasma Immunoglobulin G Responses in Elite Neutralizers of Human Cytomegalovirus
Authors: Harnois Melissa J , Dennis Maria , Stöhr Dagmar , Valencia Sarah M , Rodgers Nicole , Semmes Eleanor C , Webster Helen S , Jenks Jennifer A , Barfield Richard , Pollara Justin , Chan Cliburn , Sinzger Christian , Permar Sallie R 
Published: August 2022

AbstractBackgroundHuman cytomegalovirus (HCMV) is the most common infectious complication of organ transplantation and cause of birth defects worldwide. There are limited therapeutic options and no licensed vaccine to prevent HCMV infection or disease. To inform development of HCMV antibody-based interventions, a previous study identified individuals with potent and broad plasma HCMV-neutralizing activity, termed elite neutralizers (ENs), from a cohort of HCMV-seropositive (SP) blood donors. However, the specificities and functions of plasma antibodies associated with EN status remained undefined.MethodsWe sought to determine the plasma antibody specificities, breadth, and Fc-mediated antibody effector functions associated with the most potent HCMV-neutralizing responses in plasma from ENs (n = 25) relative to that from SP donors (n = 19). We measured antibody binding against various HCMV strains and glycoprotein targets and evaluated Fc-mediated effector functions, antibody-dependent cellular cytotoxicity (ADCC), and antibody-dependent cellular phagocytosis (ADCP).ResultsWe demonstrate that ENs have elevated immunoglobulin G binding responses against multiple viral glycoproteins, relative to SP donors. Our study also revealed potent HCMV-specific antibody-dependent cellular cytotoxicity and antibody-dependent cellular phagocytosis activity of plasma from ENs.ConclusionsWe conclude that antibody responses against multiple glycoprotein specificities may be needed to achieve potent plasma neutralization and that potently HCMV elite-neutralizing plasma antibodies can also mediate polyfunctional responses.
Engaging the disability community in informatics research: rationales and practical steps
Authors: Valdez Rupa S , Lyon Sophie E , Wellbeloved-Stone Claire , Collins Mary , Rogers Courtney C , Cantin-Garside Kristine D , Gonclaves Fortes Diogo , Kim Chung , Desai Shaalini S , Keim-Malpass Jessica , Kushalnagar Raja 
Published: August 2022

Abstract
As the informatics community grows in its ability to address health disparities, there is an opportunity to expand our impact by focusing on the disability community as a health disparity population. Although informaticians have primarily catered design efforts to one disability at a time, digital health technologies can be enhanced by approaching disability from a more holistic framework, simultaneously accounting for multiple forms of disability and the ways disability intersects with other forms of identity. The urgency of moving toward this more holistic approach is grounded in ethical, legal, and design-related rationales. Shaped by our research and advocacy with the disability community, we offer a set of guidelines for effective engagement. We argue that such engagement is critical to creating digital health technologies which more fully meet the needs of all disabled individuals.
Quantification of Anti-Osteoporotic Anabolic Peptide in Stealth Lipid Nanovesicles Through Validated RP-HPLC Method
Authors: Salave Sagar , Jain Sonali , Shah Ravi , Benival Derajram 
Published: August 2022

Abstract

Background
Teriparatide is a recombinant fragment of human parathyroid hormone, a potent osteoanabolic agent used for osteoporosis.

Objective
The present study endeavored to develop a simple, rapid, and reliable reverse phase-high performance liquid chromatography (RP-HPLC) method for the determination of teriparatide in pegylated lipid nanovesicles for rapid formulation development/optimization.

Method
A rapid RP-HPLC-based analytical method was developed for the quantification of teriparatide in pegylated lipid nanovesicles. The method was optimized on a Waters XBridge C18 (4.6 × 150 mm, 10 μm) column with a mobile phase consisting of 0.1% formic acid in water and acetonitrile both in a linear gradient program. In the method, a short run time of 9 min was achieved at a flow rate of 1.0 mL/min with an injection volume of 50 µL at a detection wavelength of 210 nm. The developed method was validated according to the ICH Q2 (R2) guideline. The method was applied for the quantification of teriparatide in prepared pegylated lipid nanovesicles. Teriparatide encapsulated pegylated lipid nanovesicles were prepared by the ethanol injection method. Further, these vesicles were characterized for % entrapment efficiency (%EE), particle size, zeta potential, and morphology by Cryo-SEM.

Results
The teriparatide was eluting at 4.8 min in the run. Further, for the method validation, the linear relationship between concentration and response was established over the concentration range of 50–250 µg/mL with the R2 &gt; 0.999. The method sensitivity was shown with LOD and LOQ with the value of 100 ng/mL and 500 ng/mL, respectively. The method was found to be accurate and precise with the recovery ranging in 100 ± 2% and RSD &lt;2%, respectively. Minor deliberate changes proved the robustness of the developed method.

Conclusions
These results indicated that the developed and validated method is accurate, precise, rapid, reliable, and fit for the quantification of teriparatide in different formulations.

Highlights
The RP-HPLC method was developed and validated for the quantification of teriparatide from novel pegylated lipid nanovesicles.
Determination of Insoluble, Soluble, and Total Dietary Fiber in Foods Using a Rapid Integrated Procedure of Enzymatic-Gravimetric-Liquid Chromatography: First Action 2022.01
Authors: McCleary Barry V , McLoughlin Ciara 
Published: August 2022

Abstract

Background
A simple, accurate, and reliable method for the measurement of total dietary fiber (TDF) according to the Codex definition (2009) was developed and successfully validated as AOAC Official Method of Analysis (OMA) 2017.16. Subsequently, OMA 2017.16 was modified to allow separate measurement of soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) fractions.

Objective
To perform a collaborative study to evaluate the repeatability and reproducibility of OMA 2017.16 modification for the measurement of total dietary fiber (TDF) as IDF and SDF measured as (1) water SDF that precipitates in 78% aqueous ethanol (SDFP), and (2) water SDF that remains soluble in 78% aqueous ethanol (SDFS) of degree of polymerization ≥3.

Methods
Duplicate test portions are incubated with pancreatic α-amylase (PAA), amyloglucosidase (AMG), and protease under the conditions employed in OMA 2017.16. For the measurement of IDF, the digestate is filtered and the IDF determined gravimetrically. SDFP in the IDF filtrate is precipitated with alcohol and captured by filtration and determined. SDFS in the SDFP filtrate is recovered and quantitated by LC. The matrixes included cereal products and flours, vegetables, health food snacks, soup, chocolate, and beans. Additional materials were analyzed by collaborators as “practice samples”.

Results
With the diethylene glycol internal standard, all multi-laboratotu (MLV) matrixes resulted in repeatability relative standard deviations (RSDr) for TDF analyses of &lt;3.60% and RSDR ranging from 4.55 to 9.26%. For the practice samples, the RSDR for TDF ranged from 6.69 to 11.68%.

Conclusion
OMA 2022.01 meets the AOAC requirements for repeatability and reproducibility and the data support First Action status.

Highlights
OMA 2022.01 is a robust and reproducible method for the analysis of insoluble, soluble (SDFP and SDFS), and TDF in a wide range of matrixes.
Glycation-induced age-related illnesses, antiglycation and drug delivery strategies
Authors: Abdelkader Hamdy , Mustafa Wesam W , Alqahtani Ali M , Alsharani Sultan , Al Fatease Adel , Alany Raid G 
Published: August 2022

Abstract

Objectives
Ageing is a major cause of multiple age-related diseases. Several mechanisms have been reported to contribute to these abnormalities including glycation, oxidative stress, the polyol pathway and osmotic stress. Glycation, unlike glycosylation, is an irregular biochemical reaction to the formation of active advanced glycation end-products (AGEs), which are considered to be one of the causes of these chronic diseases. This study provides a recent and comprehensive review on the possible causes, mechanisms, types, analytical techniques, diseases and treatments of the toxic glycation end products.

Key findings
Several mechanisms have been found to play a role in generating hyperglycaemia-induced oxidative stress including an increase in the levels of reactive oxygen species (ROS), increase in the levels of AGEs, binding of AGEs and their receptors (RAGE) and the polyol pathway and thus have been investigated as promising novel targets.

Summary
This review focuses on the key mechanisms attributed to cumulative increases of glycation and pathological RAGE expression as a significant cause of multiple age-related diseases, and reporting on different aspects of antiglycation therapy as a novel approach to managing/treating age-related diseases. Additionally, historical, current and possible future antiglycation approaches will be presented focussing on novel drug delivery methods.
The access to CETA quotas: Extending CGE models with a market for quota licenses
Authors: Döbeling Tatjana 
Published: August 2022

Abstract
We analyze the market dynamics that are caused by tariff-rate quotas, particularly the effects of quota license allocation between heterogeneous commodities at the tariff line level. The allocation is endogenously modeled with a mixed complementarity problem approach for the case of the Comprehensive Economic and Trade Agreement between Canada and the European Union. The model results are compared both with alternative models that resemble pre-existing approaches and with the real trade figures that have been collected since the trade agreement's implementation. Our analysis shows a bias toward more expensive commodities if the shadow value of a quota license manifests in a secondary license market. The same quota can thereby be binding to some commodities but not so for others. This feature of quotas can be crucial for policymakers who are concerned about price effects or who want to understand the effects of lumping together commodities of different quality in one quota.