bluuebunny/crossref_metadata_2025_split huggingface dataset:
eEF1A1 promotes colorectal cancer progression and predicts poor prognosis of patients
Authors: Fan A‐hui , Zhao Xiaojuan , Liu Hao , Li Danxiu , Guo Tongtong , Zhang Jiehao , Duan Lili , Cheng Hao , Nie Yongzhan , Fan Daiming , Zhao Xiaodi , Lu Yuanyuan 
Published: May 2022

AbstractColorectal cancer (CRC) is a major leading cause of cancer mortality worldwide in which dysregulated protein synthesis plays an etiologic role. The eukaryotic elongation factor 1 A1 (eEF1A1) exerts significant effects on protein synthesis by contributing to peptide chain extension. Whereas its role in CRC remains to be investigated. In this study, we found that the mRNA and protein levels of eEF1A1 were significantly upregulated in CRC cell lines and tissues. Elevated expression of eEF1A1 was correlated with shorter overall survival in 94 CRC patients. The inhibition of proliferation and cell cycle block were observed in CRC cells after eEF1A1 downregulation. Mechanistically, weighted gene correlation network analysis and further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that mitogen‐activated protein kinases (MAPKs) signaling pathways were significantly enriched in high‐eEF1A1 expression group, and the levels of phosphorylated p38/JNK/ERK MAPK were dramatically decreased after eEF1A1 downregulation. Overexpression of eEF1A1 in CRC correlated with a poor prognosis. Collectively, this study determined the oncogenic role of eEF1A1 in CRC proliferation and tumorigenesis. eEF1A1 might be a promising therapeutic target and prognostic biomarker in CRC.
Community‐based counselling for benzodiazepine withdrawal: A mixed‐methods study of client outcomes
Authors: Wurf Gerald , O’Neal Paul 
Published: May 2022

AbstractDespite adverse health outcomes being associated with long‐term tranquilliser use, health professionals face numerous barriers in reducing reliance on benzodiazepines. This study investigated the effectiveness of focused counselling in facilitating benzodiazepine withdrawal. In phase one of a two‐phase mixed‐methods evaluation, preintervention and postintervention quantitative data for 24 participants were analysed. Measures included the Australian Treatment Outcome Profile, Kessler 10, and two client outcome ratings. In phase two, follow‐up individual interviews were conducted with six participants. Following counselling, 88% of participants reported either reduced use or withdrawal from benzodiazepines. Significant reductions in psychological distress were obtained in Kessler 10 scores, and the calculated effect size (d = 0.84) was large. Medically supervised tapering was well received when combined with focused counselling. We found strong support for the tapering process and for the appropriateness of counselling. Withdrawal was facilitated when services were well coordinated and the client's sense of control was maintained.
Acute liver failure due to hepatitis B virus reactivation induced by doxorubicin and cyclophosphamide chemotherapy for adjuvant treatment of breast cancer: A case report
Authors: Hakami Tahir 
Published: May 2022

AbstractAcute liver failure developed in a 48‐year‐old woman within days after she received adjuvant chemotherapy for breast cancer. On arrival at ED, she had severe encephalopathy and jaundice. Serum analyses demonstrated coagulopathy and markedly increased transaminases. She was admitted to the ICU for supportive treatment but died several days later.
Intrapelvic migration of the lag screw in intramedullary nailing after intertrochanteric fracture fixation: A case report
Authors: Ben Fredj Aymen , Rbai Hedi , Farhat Fourat , Berriri Marouen 
Published: May 2022

AbstractThe expected number of hip fractures worldwide will rise from 1.7 million in 1990, to 6.3 million in 2050. In parallel, surgical complications are expected to increase. We describe a rare complication of intrapelvic migration of the lag screw from trochanteric nail in a 90‐year‐old woman.
A consensual qualitative analysis of counselor educators’ experiences incorporating neuroscience
Authors: Beijan Lisa L. , Prosek Elizabeth A. , Jones Leslie D. , Jackson Danielle , Legacy Brittany 
Published: May 2022

AbstractWe used consensual qualitative research to explore eight counselor educators’ experience integrating neuroscience into counseling curriculum. Six domains, 18 categories, and 37 subcategories emerged from the data highlighting barriers, successes, and future trajectory of incorporating neuroscience consistently and ethically into counselor training.
Two‐Dimensional Detergent Expansion Strategy for Membrane Protein Studies
Authors: Zhao Fei , Zhu Zhihao , Xie Linshan , Luo Feng , Wang Huixia , Qiu Yanli , Luo Weiling , Zhou Fang , Xue Dongxiang , Zhang Zhihui , Hua Tian , Wu Dong , Liu Zhi‐Jie , Le Zhiping , Tao Houchao 
Published: May 2022

AbstractDetergents are the most frequently applied reagents in membrane protein (MP) studies. The limited diversity of one‐head‐one‐tailed traditional detergents, however, is far from sufficient for structurally distinct MPs. Expansion of detergent repertoire has a continuous momentum. In line with the speculation that detergent pre‐assembly exerts superiority, herein we report for the first time cross‐conjugation of two series of monomeric detergents for constructing a two‐dimensional library of dimeric detergents. Optimum detergents stood out with unique preferences in the systematic evaluation of individual MPs. Furthermore, unprecedented hybrid detergents 14M8G and 14M9G enabled high‐quality EM study of transporter MsbA and NMR study of G protein‐coupled receptor A2AAR, respectively. Given the abundance of cross‐coupling chemistries, comprehensive diversity could be readily covered that would facilitate the finding of new detergents for the manipulation of thorny MPs and innovation of the functional and structural study in future.
Manipulator trajectory tracking based on adaptive sliding mode control
Authors: Zhao Haoyi , Tao Bo , Ma Ruyi , Chen Baojia 
Published: May 2022

AbstractA manipulator is a complex electromechanical system that is nonlinear, strongly coupled, and uncertain. Achieving its precise and high‐quality trajectory control is difficult. Sliding mode control (SMC) is one of the common control methods for manipulators. However, discontinuities in SMC can cause jitter and vibration in the manipulator system, leading to a reduction in the performance of the control system. For the self‐adaptive capability jitter vibration problem of SMC, the Dobot magician manipulator is treated as the research object in this article. The dynamics equations of the manipulator are established by Lagrange method, and a simplified model of the manipulator dynamics is constructed. The method of self‐adaptive sliding mode control is proposed. Self‐adaptive parameters are added to the SMC to achieve self‐adaptive adjustment of the SMC parameters. In the MATLAB/Simulink simulation environment analysis show that the self‐adaptive SMC method has better self‐tuning ability and trajectory tracking ability than the traditional SMC, and weakens the jitter phenomenon existing in the traditional SMC.
Solvent Effect on the Chemiluminescence of Acridinium Thioester: A Computational Study
Authors: Pieńkos Milena , Zadykowicz Beata 
Published: May 2022

AbstractChemiluminescent labelling, which is one of the promising procedures of modern immunodiagnostics, is increasingly carried out using acridinium derivatives, an oxidant, and an alkaline aqueous environment. However, the efficiency of the chemiluminescence of luminol or acridinium esters is higher in non‐aqueous solvents such as dimethyl sulfoxide or acetonitrile. Therefore, the search for a new environment for the chemiluminescence reaction, especially the one characterized by a higher quantum yield of chemiluminescence, is one of the aims of current research. Using computational methods (DFT and TD DFT with PCM model of solvent), we examined thermodynamic and kinetic data concerning the chemiluminescence and competitive dark pathways. Our results suggest that better characteristics of the chemiluminescence reaction of acridinium thioester are observed in nonpolar solvents, such as methylcyclohexane, n‐hexane and n‐pentane, than in aqueous media used so far. Further experimental verification is necessary to confirm the possible application of proposed nonpolar solvents in chemiluminescent labelling and hence in immunodiagnostics.
Species‐specific DNA distribution in spruce–beech forest soil
Authors: Foscari Alessandro , Alberti Giorgio , Zotti Maurizio , Incerti Guido 
Published: May 2022

AbstractEnvironmental DNA consists of species‐specific intracellular and extracellular fractions, whose content and information may not be similar in all environments. In forest soil, in particular, the biogeochemical fate of DNA originated by plant litter input has been extensively reviewed, but species‐specific persistence and distribution still await to be quantified. In the present work, based on the purification of extracellular and intracellular DNA fractions from forest soil samples representing 3 soil horizons at 36 randomized locations differing for stand composition (either beech‐ or spruce‐dominated, and mixed), followed by exDNA metabarcoding with the rbcL marker, we provide a clear picture of species‐specific plant DNA distribution, and explore plant community composition and diversity along the explored gradient and the soil profile. We did not find significant differences in intra‐ vs. extracellular total DNA distribution, with a progressive depletion with soil depth positively associated with soil organic C and N content and negatively associated with soil pH and mineral content. Species‐specific DNA distribution was horizontally dependent on beech and spruce basal area aboveground, while extracellular DNA showed peculiar species‐specific vertical patterns. Proportion of Fagus sylvatica DNA increased with depth in beech stand soil, and Picea abies DNA decreased in spruce stand soil, respectively, possibly linked to species‐specific differences in leaf litter decomposition dynamics and root litter contributions. Finally, our approach by metabarcoding provided a faithful, although incomplete, picture of the local plant diversity, suggesting that such technique could positively integrate traditional biodiversity inventory studies based on expert field assessments.
Synthesis of Triple‐Stranded Diruthenium(II) Compounds
Authors: Flint Kate L. , Huang David M. , Linder‐Patton Oliver M. , Sumby Christopher J. , Keene F. Richard 
Published: May 2022

AbstractA series of ligands containing a 1,4‐disubstituted 1,2,3‐triazole unit have been used for the formation of triple‐stranded dinuclear Ru(II) complexes. In contrast to the previously reported complexes of labile metals, the use of inert Ru(II) enabled stereoisomeric mixtures of triple‐stranded diruthenium(II) complexes to be accessed. The chromatographic resolution of the enantiomers of a reported helicate containing a more rigid 1,4‐xylyl spacer was carried out on cellulose. The ligand spacer was modified and as the flexibility increased the production of isomeric mixtures was detected; the mesocate and helicate forms were separated when an n‐propyl spacer was used. This pair of diastereomers was found to exhibit photoconversion, a unique observation for Ru(II) compounds of this type. Partial separation via chromatographic resolution was achieved for compounds containing an n‐butyl spacer, and the presence of a mesocate/helicate pair confirmed.
Cooperative Rh(II)/Pd(0) Dual‐Catalyzed gem‐Difunctionalization of α‐Diazo Carbonyl Compounds: Construction of Quaternary Carbon Centers
Authors: Xuan Zi , Chen Zi‐Sheng 
Published: May 2022

AbstractThe efficient construction of quaternary carbon centers is a challenging task due to their enhanced structural complexity. Although the catalytic gem‐difunctionalization of α‐diazo carbonyl compounds via metal‐carbenoids is well‐documented, developing new methods to access quaternary carbon centers which are difficult or unattainable in monocatalysis is highly desirable. Dual catalysis where two different catalysts work concurrently has emerged as a powerful strategy to achieve new reactivity and selectivity. In this review, we highlight the recent advances in cooperative Rh(II)/Pd(0) dual catalysis for the construction of quaternary carbon centers, focusing on the synthetic utility of stable α‐diazo‐β‐keto‐esters and allylic carboxylates. Additionally, several challenging asymmetric Rh(II)/Pd(0) dual‐catalyzed transformations generating chiral quaternary carbon centers are also discussed.
Brain serotonin transporter is associated with cognitive‐affective biases in healthy individuals
Authors: Armand Sophia , Ozenne Brice , Svart Nanna , Frokjaer Vibe G. , Knudsen Gitte M. , Fisher Patrick M. , Stenbæk Dea S. 
Published: May 2022

AbstractCognitive affective biases describe the tendency to process negative information or positive information over the other. These biases can be modulated by changing extracellular serotonin (5‐HT) levels in the brain, for example, by pharmacologically blocking and downregulating the 5‐HT transporter (5‐HTT), which remediates negative affective bias. This suggests that higher levels of 5‐HTT are linked to a priority of negative information over positive, but this link remains to be tested in vivo in healthy individuals. We, therefore, evaluated the association between 5‐HTT levels, as measured with [11C]DASB positron emission tomography (PET), and affective biases, hypothesising that higher 5‐HTT levels are associated with a more negative bias. We included 98 healthy individuals with measures of [11C]DASB binding potential (BPND) and affective biases using The Emotional Faces Identification Task by subtracting the per cent hit rate for happy from that of sad faces (EFITAB). We evaluated the association between [11C]DASB BPND and EFITAB in a linear latent variable model, with the latent variable (5‐HTTLV) modelled from [11C]DASB BPND in the fronto‐striatal and fronto‐limbic networks implicated in affective cognition. We observed an inverse association between 5‐HTTLV and EFITAB (β = −8% EFITAB per unit 5‐HTTLV, CI = −14% to −3%, p = .002). These findings show that higher 5‐HTT levels are linked to a more negative bias in healthy individuals. High 5‐HTT supposedly leads to high clearance of 5‐HT, and thus, a negative bias could result from low extracellular 5‐HT. Future studies must reveal if a similar inverse association exists in individuals with affective disorders.
How access to addictive drugs affects the supply of substance abuse treatment: Evidence from Medicare Part D
Authors: Knowles Matthew T. 
Published: May 2022

AbstractThis paper documents how substance abuse treatment (SAT) providers and services respond to increases in population‐level opioid addiction. I do this by exploiting the implementation of Medicare Part D as an exogenous increase in the availability of prescription opioids. Starting in 2006, states with higher shares of the population eligible for Medicare Part D experienced increases in residential and hospital inpatient SAT facilities, beds dedicated to SAT, and SAT facilities offering medication‐assisted treatment, relative to states with lower shares. These results suggest that the supply of SAT in the United States is capable of responding significantly to changes in demand.
Risk factors for spontaneous preterm birth among healthy nulliparous pregnant women in the Netherlands, a prospective cohort study
Authors: Schuster Heleen J. , Peelen Myrthe J. C. S. , Hajenius Petra J. , van Beukering Monique D. M. , van Eekelen Rik , Schonewille Marit , Playfair Henna , van der Post Joris A. M. , Kok Marjolein , Painter Rebecca C. 
Published: May 2022

AbstractIntroductionSpontaneous preterm birth (sPTB) is a major contributor to neonatal morbidity and mortality worldwide. The pathophysiology of sPTB is poorly understood, in particular among nulliparous women without apparent medical or obstetric risk factors. Therefore, we aimed to identify risk factors for sPTB in healthy nulliparous women.Material and MethodsWe performed a prospective cohort study. Recruitment took place from February 2014 to December 2016 in 16 community midwifery centers in the Netherlands. Eligibility criteria were: ≥18 years, no previous pregnancy >16 weeks of gestation, healthy singleton pregnancy, and antenatal booking <24 weeks of gestation. At study inclusion, participants completed a questionnaire, including details on lifestyle, work, and medical history. Cervical length was measured by vaginal ultrasound at the second‐trimester anomaly scan. Detailed information concerning pregnancy and birth was collected via antenatal charts. We calculated the adjusted odds ratio (aOR) and 95% confidence intervals (CI) for various risk factors with correction for socioeconomic status (SES) using logistic regression and Firth's correction.ResultsWe included 363 women of whom pregnancy outcomes were available in 349 (96.1%) participants. The cervical length measurement was available for 225 (62.0%) participants. sPTB occurred in 26 women (7.5%). SES was associated with sPTB (OR: 3.7, 95% CI: 1.6–8.5) in univariate analysis. First or second trimester vaginal bleeding (aOR: 3.6, 95% CI: 1.4–9.0) and urinary tract infection during pregnancy (aOR: 4.9, 95% CI: 1.7–13.9) were associated with sPTB in multivariate analysis.ConclusionsThis prospective cohort confirms established risk factors for sPTB in nulliparous women deemed at low risk of sPTB.
The impact of community closures among nonessential and essential workers, Nashville, Tennessee: A cross‐sectional study
Authors: Rankin Danielle A. , Yanis Ahmad , Haddadin Zaid , Talj Rana , Fernandez Kailee N. , Bloos Sean M. , Stahl Anna , Gu Wenying , Nicotera Janet , Howe Harrison L. , Salib Seifein , Chappell James , Howard Leigh M. , Khankari Nikhil K. , Halasa Natasha B. 
Published: May 2022

AbstractBackground and AimsThe effects of community closures and relaxing social distancing restrictions on severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) by occupational risk remain unclear. Therefore, we evaluated the impact of community closures and reopening phases with the prevalence of testing SARS‐CoV‐2‐positive among nonessential and essential workers.MethodsWe constructed a cross‐sectional cohort from March 20 to July 31, 2020, of 344 adults from Metropolitan Nashville, Tennessee. We performed an unconditional logistic regression model to evaluate the impact of community closures and phase implementation on testing SARS‐CoV‐2 positive by occupation to estimate adjusted prevalence odds ratios (aPORs) and 95% confidence intervals (CIs).ResultsDuring a stay‐at‐home/Phase I order, those with non‐essential occupations had 59% decreased prevalence odds (aPOR:0.41; 95% CI: 0.20–0.84) of testing SARS‐CoV‐2‐positive compared to when no restrictions were in place. Persons with essential occupations had four times the prevalence odds of testing SARS‐CoV‐2‐positive (aPOR:4.19; 95% CI:1.57–11.18) compared with nonessential occupations when no community restrictions were established.ConclusionStay‐at‐home restrictions were associated with a lower risk of SARS‐CoV‐2 infection in the community for nonessential workers. Essential employees remained at increased risk for SARS‐CoV‐2, including when no community restrictions were in place and vaccines were not available. This study supports targeting prevention measures for these high‐risk occupations.
Over‐the‐counter drug use in suicidal/self‐harm behavior: Scoping review
Authors: Shoib Sheikh , Patel Vraj , Khan Sonia , Armiya'u Aishatu Yusha'u , Saeed Fahimeh , Swed Sarya , Das Soumitra , Chandradasa Miyuru 
Published: May 2022

AbstractBackground and aimsMinor physical ailments are treated with over‐the‐counter (OTC) medications. The availability of OTC drugs helps reduce waiting times and ease the suffering of many. Suicidal behavior includes suicidal ideation, attempts, and completed suicides and affects people of all ages, religions, and cultures. This study aims to review use of OTC drugs for self‐harm and suicidal attempts.MethodsWe reviewed English language publications from the beginning of time to October 2021 on OTC drug use for suicidal behavior.ResultsTwenty‐seven studies met the eligibility criteria, and 1,816,228 participants were reported in these publications. OTC analgesics and sedatives/hypnotics were frequently used for suicidal behavior. Females and young people mainly were reported to self‐harm using OTC medications. An increase in OTC analgesic use for self‐harm in adolescents during the school months was reported. Elderly persons use hypnotics more frequently for suicidal attempts. Persons with major psychiatric disorders were reported to use OTC for suicidal behavior.ConclusionThe available information shows that the prevention strategies should focus on OTC analgesics and hypnotic use among women, the young, the elderly, and persons with mental health disorders.
Repurposing PDE5 inhibitor tadalafil and sildenafil as anticancer agent against hepatocellular carcinoma via targeting key events of glucose metabolism and multidrug resistance
Authors: Chhonker Saurabh Kumar , Rawat Divya , Koiri Raj Kumar 
Published: May 2022

AbstractHepatocellular carcinoma (HCC) has emerged as one of the most common and lethal cancers worldwide and is caused due to contamination of diets with aflatoxin B1 and chronic viral hepatitis. Recent reports suggest that phosphodiesterase‐5 inhibitor (PDE5i) exhibits anticancer properties against several forms of cancer but till now has not been evaluated against HCC. We aimed to evaluate the anticancer property of phosphodiesterase‐5 inhibitors (PDE5i) tadalafil and sildenafil against aflatoxin B1 HCC. Rats of HCC group were fed with 5% alcohol via drinking water for 3 weeks, followed by administration of AFB1 (1 mg/kg/bw, i.p.) at an interval of two subsequent days. PDE5i (tadalafil and sildenafil, 10 mg/kg bw) was administered along with drinking water after 6 weeks of treatment with AFB1 for 2 weeks. In the present investigation, in HCC elevation in the level of SGOT, SGPT, ALP, and urea vis‐à‐vis activity of key glycolytic enzyme LDH and mRNA expression of c‐myc, Akt, LDH‐A, and PFKFB3 was noted. Similarly, the level of multidrug resistance protein (MDR) and breast cancer resistance protein (BCRP/ABCG2) was elevated along with increased expression of angiogenesis marker (HIF‐1α, VEGF, and TGF‐β1) in HCC. Post‐treatment with PDE5 inhibitor (tadalafil and sildenafil) downregulated and brought back the above parameters towards normal and out of two PDE5i (tadalafil and sildenafil), sildenafil effect was more potent as compared to tadalafil. Our findings demonstrate for the first time that PDE5 inhibitors tadalafil and sildenafil are able to prohibit the development and progression of aflatoxin B1 induced HCC.