bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of different concentrations of reversine to enhance conversion of dedifferentiated fat cells into mature cardiomyocytes
Authors: Dharmadjati Budi Baktijasa , Sargowo Djanggan , . Aulanni’am , Pikir Budi Susetyo , Oktaviono Yudi Her , Sativa Oryza , Arjani Kandita , Nugraha Ricardo Adrian 
Published: July 2022

Background: There is an essential need for cardiomyocyte regeneration among patients with heart failure. Transplantation of dedifferentiated fat (DFAT) cells may lead to an improvement of cardiomyocyte regeneration among heart failure patients. We believe that DFAT cells are promising candidate cell sources for cardiac regeneration. However, the pathway underlying how DFAT cells of the adipose lineage differentiate into mature cardiomyocytes isn’t fully understood. Methods: We conducted an experimental laboratory study on isolated DFAT cells from adipose tissue of healthy adults. Then, we treated cells with different concentrations of reversine (10, 20 and 40 nM), and performed RNA extraction and cDNA synthesis. Next, we used a ceiling culture method based on the buoyancy properties of mature lipid-filled adipocytes. Stemness expression (Octamer-binding transcription factor 4 [Oct4], brachyury, Fetal liver kinase 1 [Flk-1]) was quantified by reverse transcription-quantitative (RT-q)PCR, while cardiomyocyte expression (Transcription factor GATA-4 [GATA4] and cardiac troponin T [cTnT]) was quantified by immunocytochemistry. Results: ANOVA with Tukey’s post-hoc found that 10 nM reversine increased greater Flk-1 expression compared to the control group (MD: 5.037 + 0.998; p < 0.001), but there were no significant changes among Oct4 (MD: 0.013 + 1.244; p = 0.99) and brachyury expression (MD: 0.157 + 0.084; p = 0.252). Kruskal-Wallis revealed that the expression of GATA4 (1.65 [0.41-1.98] to 0.015 [0.007-0.034]; p = 0.017) reduced significantly from day 7 until day 21 and cTnT (5.07 [6.62-8.91] to 8.22 [6.81-9.40]; p = 0.001) increased significantly from day 7 until day 21. Conclusions: Reversine could increase the expression of Flk-1, but it was unable to stimulate the expression of Oct4 and brachyury related to stem cell-ness. An optimal concentration of 10 nM reversine may have the greatest effect on enhancing the differentiation of DFAT cells into mature cardiomyocytes, as indicated by higher cTnT expression between cells.
The burden of BRCA1 and BRCA2 gene mutations among Vietnamese women and their associated factors: A protocol for a systematic review and meta-analysis
Authors: Tran Phuc Thai , Tran Duc Quang , Vu Chi Thi Quynh , Phan Quang Ngoc , Nguyen Anh Thi Thu 
Published: July 2022

Background: BReast CAncer gene (BRCA)1 and BRCA2 mutation carriers are frequently provided genetic counselling. A precise estimation of the prevalence of BRCA gene mutations is essential to provide an appropriate risk prediction. However, the data in Vietnam is ambiguous and include unreliable risk factors from individual studies. Hence, the objective of this protocol is to provide a method to synthesize evidence pertaining to the proportion of BRCA mutations among Vietnamese women and their risk factors for cancer. Methods: PRISMA-P was followed in developing and reporting this protocol. From the databases' inception until June 2023, a comprehensive search will be undertaken in electronic PubMed and Scopus databases. In two stages, title/abstract screening and full-text screening, two independent authors will assess all retrieved articles for inclusion. This review includes papers providing the relevant results reflecting the prevalence of BRCA gene mutations in Vietnamese women who are at least 18 years old with or without cancer. Predefined selection criteria will be used to establish each publication's eligibility. Using the Newcastle-Ottawa Scale and Cochrane Risk of Bias tools, the quality of included studies will be assessed, and overall evidence quality will be appraised using the GRADE approach. All pertinent data from the included articles will be entered into an Excel spreadsheet for meta-analysis, which will be imported into Rstudio. Meta-analyses using random effects will be used to obtain the pooled percentages. The chi-squared test and I2 parameter will be used to evaluate heterogeneity. Publication bias will be investigated visually using funnel plots for asymmetry and Egger's statistical tests. Conclusions: Based on the prevalence of BRCA muations and its associated comprehensive and specific risk factors, we hope our findings will provide a reference for future strategies to build an effective treatment plan and manage the risk of cancer development. Registration: PROSPERO (CRD42022340152; 30 June 2022).
PARTISIPASI MASYARAKAT DALAM PENGELOLAAN SAMPAH TERPADU DI SUMBERAGUNG JETIS BANTUL DIY
Authors: Wijayanti Nor , Romas Arina Nuraliza 
Published: July 2022

Latar Belakang: Sampah telah menjadi permasalahan nasional sehingga mengelolaannya perlu dilakukan secara komprehensif dan terpadu dari hulu ke hilir agar memberikan manfaat secara ekonomi, sehat bagi masyarakat dan aman bagi lingkungan serta dapat mengubah perilaku masyarakat.. Permasalahan yang terjadi di Sumberagung Jetis Bantul, berdasarkan informasi dari pengelola sampah, diperoleh hasil bahwa antusias dari masyarakat mengenai pengelolaan sampah tergolong rendah yaitu sekitar 30%, masyarakat tidak turut berpartisipasi dalam pemilahan sampah dan pendaur ulangan sampah.Tujuan:untuk mengetahui pengaruh motivasi, komunikasi, kooperasi dan mobilisasi terhadap partisipasi masyarakat dalam pengelolaan sampah terpadu di Sumberagung, Jetis, Bantul, DIY.Metode:Desain penelitian ini adalah desain studi korelasional dengan pendekatan cross sectional. Sampel sebanyak 59 masyarakat di Sumberagung Jetis Bantul dipilih menggunakan purposive sampling. Data dianalisis dengan menggunakan regresi linear berganda.HasilÂ Â Â :Terdapat pengaruh secara parsial antara komunikasi terhadap partisipasi masyarakat dalam pengelolaan sampah terpadu yaitu nilai Ï = 0,021 < Î± = 0,05 dan terdapat pengaruh secara stimultan antara motivasi, komunikasi, kooperasi dan mobilisasi terhadap partisipasi masyarakat dalam pengelolaan sampah terpadu yaitu nilai Ï = 0,000< Î± = 0,05.Kesimpulan: Ada pengaruh secara parsial antara komunikasi terhadap partisipasi masyarakat dalam pengelolaan sampah terpadu dan ada pengaruh secara stimultan antara motivasi, komunikasi, kooperasi dan mobilisasi terhadap partisipasi masyarakat dalam pengelolaan sampah terpadu.Â Kata Kunci: Motivasi, Komunikasi, Kooperasi, Mobilisasi, Partisipasi Pengelolaan Sampah, Masyarakat
EFEKTIVITAS PENGGUNAAN BEBERAPA MEREK CAIRAN PENCUCI TERHADAP PENURUNAN JUMLAH ANGKA KUMAN PIRING
Authors: Adelia Adelia , Tri Sukesi Wahyuni 
Published: July 2022

abstracâ€” Washing the cutlery is one of most simple and common ways to maintain the cleanliness of cutlery in order to fulfill the requirements about the number of germs that is 0 (zero) from the cutlery surface. Dishwashing liquid is a necessary product in the washing process. Dishwashing liquid in the market consists of a various brands, which causes the household to use different dishwashing liquid products. This study aims to determine effectiveness of using several brands diswashing liquids against decreaseÂ the number of germ on plate.This research type is a quasi experiment with pretest-posttest with control group design. The subjects of this research is the number of germ and the object is plate with treatment of A, B, C, D and E brand dishwashing liquid with three replications. Analisys data use paired sample t-test and kruskall wallis non parametric test.The result of bivariate analysis with paired sample t-test showed there was difference of germs number before and after treatment with A, B and C dishwashing liquid (p <0,05) and there was no difference of germs number before and after treatment with D, E dishwashing liquid and control group (p> 0.05) statistically. The result of kruskal wallis test showed that there was no significant difference against decrease the germs number on plate by using some brands of dishwashing liquid with p value = 0,506.The conclusion of this research is no significant difference against decrease the number of germs on plate by using several brands of dishwashing liquid. There is need to do further research of washing process by adding desinfection process.Â
ANALISIS RIWAYAT BERAT BADAN LAHIR RENDAH (BBLR) DENGAN PERKEMBANGAN ANAK USIA BALITA: SISTEMATIC LITERATURE REVIEW
Authors: Wibiyani Artiyan Agi , Gustina Erni 
Published: July 2022

The prevalence of low birth weight (LBW) is 15% of all births in the world and mostly occurs in developing countries. Based on statistical analysis shows that 90% of LBW events occur in developing countries and the mortality rate reaches 35 times compared to babies born with normal weight. Long-term problems that can occur in children who have a history of LBW include growth and development. The purpose of the study was to determine the relationship between a history of LBW and the development of children under five. Systematic literature review was used in this study. The search strategy used 2 electronic databases, namely PubMed and Google Scholar and followed the PRISMA (Prefferes Reporting Items For Systematic Review And Meta Analyses) protocol, with a publication period of 2011-2020. There were 18 articles that matched the inclusion and exclusion criteria. The results of the study of research articles found that there was a relationship between a history of LBW and the development of children under five. The paper discusses development that includes gross motor, fine motor, speech and language development, as well as social and independent development. Based on the article review, besides being caused by low birth weight, development can also be influenced by several other factors including parental stimulation, nutrition, and the sex of the child. Children with a history of LBW are at greater risk of experiencing slower growth and development than infants with normal weight. The impact is the failure to grow and develop according to age. Families with babies who have a history of LBW are expected to always provide more optimal health efforts by utilizing health service facilities so that children can grow and develop properly.Abstrak dibuat maksimal terdiri dari 200 kata, meliputi tujuan, metode, hasil, pembahasan dan kesimpulan dilakukannya penelitian. Abstrak tidak boleh berisi tabel/gambar, tidak perlu mencantumkan sitasi. Hindari singkatan seminimal mungkin. (font: Times New Roman 10, cetak normal, posisi rata kanan kiri, bahasa latin dengan cetak miring, berbahasa Inggris).
ANALISIS FAKTOR RISIKO PELAKSANAAN PEMBERANTASAN SARANG NYAMUK DEMAM BERDARAH DENGUE (PSN-DBD) SELAMA PANDEMI COVID-19 DI WILAYAH DESA CATURTUNGGAL DEPOK SLEMAN
Authors: Sukesi Tri Wahyuni , Ambarwati Vicky Dita 
Published: July 2022

DHF is still a health problem in the Special Region of Yogyakarta as evidenced by an increase in the CFR rate in 2019 by 0.2%, increasing to 0.4% in 2020. The incidence rate of DHF in Sleman Regency in 2019 was 67.97%. This figure still exceeds the indicators for the strategic plan of the Ministry of Health of the Republic of Indonesia. In addition, the COVID-19 pandemic since 2019 has also demanded the government and the public to be able to adapt to the new-normal as it is today, especially with regulations restricting activities outside the home to break the chain of transmission of COVID-19. During a pandemic like today, apart from the high number of COVID-19 cases, the government is still faced with other health problems, namely the high number of dengue case. The type of research used in this research is quantitative method using a cross sectional research design. The results of the study explained that there was no relationship between knowledge and behavior of PSN-DHF during the COVID-19 pandemic in Santren Hamlet, Caturtunggal Village, Depok Sleman with a p value of 1,000 (p > 0.05). There is no relationship between attitude and PSN-DHF behavior during the COVID-19 pandemic in Santren Hamlet, Caturtunggal Village, Depok Sleman with a p value of 1,000 (p > 0.05). There is no relationship between work activity and PSN-DHF behavior during the COVID-19 pandemic in Santren Hamlet, Caturtunggal Village, Depok Sleman with a p value of 0.872 (p > 0.05). There is no relationship between knowledge and behavior, there is no relationship between attitude and behavior, there is no relationship between work activities and PSN-DHF behavior during the COVID-19 pandemic in Santren Hamlet, Caturtunggal Village, Depok Sleman.
Digital competency: a survey of UK allied health professionals
Authors: Tack Christopher , Holdsworth Lesley , Wilson Alex , McComiskie Euan , McCabe Pippa , Wilkinson Wendy , King Mathew 
Published: July 2022

Background/Aims The need to develop digital skills and confidence among NHS staff is reflected in research and government strategies across the UK. This study aimed to measure the confidence, motivation and competence of the allied health professional workforce in relation to data and digital technology. Methods A quantitative, cross-sectional design was used, with an online survey distributed to measure respondents' characteristics and their self-rating of confidence, motivation and experience in data and digital technology, and of competence relative to the domains of the Health Education England allied health professions digital competency framework. Mean scores were calculated to measure self-rated confidence, motivation and experience. Results Mean scores on confidence and motivation to use digital technologies at work were moderate to high (7.34/10 and 8.33/10 respectively). High degrees of competence and applicability were reported in the ‘general’ and ‘data management and clinical informatics’ domains. Conversely, in the ‘decision support’ domain, 79.6% (n=490) of respondents reported that the competencies were applicable, but also had low competency rates (64.6%, n=397). In the domains of ‘meta competency’ and ‘records, assessments and plans’, 48.3% and 91% of respondents respectively reported low competence. Conclusions Allied health professions have a moderate–high degree of confidence and motivation towards engaging with digital technologies in practice. However, self-perceived competence in some aspects of the domains of the digital competency framework is lacking. Workforce development programmes should consider learning needs related to electronic health records, decision support and use of machine learning or artificial intelligence. Focus should also be directed towards the development of digital leadership and strategy development skills.
Coagulation abnormalities in children with uncorrected congenital heart defects seen at a teaching hospital in a developing country
Authors: Majiyagbe Omotola O. , Akinsete Adeseye M. , Adeyemo Titilope A. , Salako Abideen O. , Ekure Ekanem N. , Okoromah Christy A. N. 
Published: July 2022

Background
Coagulation abnormality is a significant complication and cause of mortality in children with uncorrected congenital heart defects (CHD). The aim of this study was to determine the prevalence of coagulation abnormalities and the associated factors in children with uncorrected CHD.

Method
A cross sectional study conducted to determine the prevalence of coagulation abnormalities among 70 children with uncorrected CHD aged six months to 17 years and 70 age and sex matched apparently healthy controls. Coagulation abnormalities was determined using complete blood count, prothrombin time, activated partial thromboplastin time and D-dimer assay.

Results
The prevalence of coagulation abnormalities among children with CHD and controls was 37.1% and 7.1% respectively. Children with Cyanotic CHD had a significantly higher prevalence of coagulation abnormalities compared to children with Acyanotic CHD (57.1% versus 17.1%). Haematocrit and oxygen saturation levels were significantly associated with coagulation abnormalities.

Conclusion
This study affirms that coagulation abnormalities are frequent in children with uncorrected CHD. Oxygen saturation and haematocrit are risk factors of coagulation abnormalities. Routine coagulation screen is recommended especially in children with cyanotic congenital heart defects to improve their quality of life and reduce morbidity and mortality while awaiting definitive surgeries.
A machine learning model on Real World Data for predicting progression to Acute Respiratory Distress Syndrome (ARDS) among COVID-19 patients
Authors: Lazzarini Nicola , Filippoupolitis Avgoustinos , Manzione Pedro , Eleftherohorinou Hariklia 
Published: July 2022

Introduction
Identifying COVID-19 patients that are most likely to progress to a severe infection is crucial for optimizing care management and increasing the likelihood of survival. This study presents a machine learning model that predicts severe cases of COVID-19, defined as the presence of Acute Respiratory Distress Syndrome (ARDS) and highlights the different risk factors that play a significant role in disease progression.

Methods
A cohort composed of 289,351 patients diagnosed with COVID-19 in April 2020 was created using US administrative claims data from Oct 2015 to Jul 2020. For each patient, information about 817 diagnoses, were collected from the medical history ahead of COVID-19 infection. The primary outcome of the study was the presence of ARDS in the 4 months following COVID-19 infection. The study cohort was randomly split into training set used for model development, test set for model evaluation and validation set for real-world performance estimation.

Results
We analyzed three machine learning classifiers to predict the presence of ARDS. Among the algorithms considered, a Gradient Boosting Decision Tree had the highest performance with an AUC of 0.695 (95% CI, 0.679–0.709) and an AUPRC of 0.0730 (95% CI, 0.0676 – 0.0823), showing a 40% performance increase in performance against a baseline classifier. A panel of five clinicians was also used to compare the predictive ability of the model to that of clinical experts. The comparison indicated that our model is on par or outperforms predictions made by the clinicians, both in terms of precision and recall.

Conclusion
This study presents a machine learning model that uses patient claims history to predict ARDS. The risk factors used by the model to perform its predictions have been extensively linked to the severity of the COVID-19 in the specialized literature. The most contributing diagnosis can be easily retrieved in the patient clinical history and can be used for an early screening of infected patients. Overall, the proposed model could be a promising tool to deploy in a healthcare setting to facilitate and optimize the care of COVID-19 patients.
Magnitude and factors associated with treatment non-adherence among patients with depressive disorders at St. Amanuel Mental Specialized Hospital, Addis Ababa, Ethiopia, 2019: A cross sectional study
Authors: Alemayehu Yadeta , Asfaw Henock , Girma Million 
Published: July 2022

Background
Inadequate adherence to treatment is among the main underlying causes of depression becoming a chronic problem. In developing countries due to limited access to health care, inaccurate diagnoses, and scarcity of medications, poor adherence may become an even larger obstacle in the treatment of depression. The current study aims to assess the magnitude and factors related to treatment non-adherence among patients with depressive disorders.

Objective
To assess the magnitude and factors associated with treatment non adherence among patients with depressive disorders at St. Amanuel Mental Specialized Hospital, Addis Ababa, Ethiopia, 2019.

Methods
A hospital-based cross-sectional study was conducted among 415 respondents using systematic random sampling technique. Medication adherence was assessed by using Medication Adherence Rating Scale. Data was entered to Epi-data version 3.1 and analyzed using SPSS version 20. Binary logistic analysis was done and P-values less than 0.05 were considered statistically significant.

Results
The prevalence of treatment non-adherence among patients with depressive disorders was 26% (95%CI; 21.2, 32.5). Previous suicide attempt (AOR = 3.05, 95%CI; 1.82, 5.12), medication side effects (AOR = 2.46, 95%CI; 1.47, 4.11), moderate to high self-stigma (AOR = 2.60, 95%CI; 1.45, 4.66), and poor quality of life (AOR = 2.47, 95%CI; 1.42, 4.28) were significantly associated with treatment non-adherence among patients with depressive disorders.

Conclusion and recommendation
Treatment non-adherence is a common problem among patients being treated for depressive disorders. Previous suicide attempts, medication side effects, moderate to high self-stigma, and poor quality of life were significantly associated with treatment non-adherence. Appropriate interventions should be developed to promote measures to facilitate adherence in this group of patients, and address the associated factors when applicable.
Malaria prevalence and risk factors among patients visiting Mizan Tepi University Teaching Hospital, Southwest Ethiopia
Authors: Duguma Tadesse , Tekalign Eyob , Muleta Dassalegn , Simieneh Asnake 
Published: July 2022

Background
Ethiopia is among sub-Saharan African countries with a high number of malaria cases each year, with most of the landmass favoring the breeding of the vectors. There have been extensive efforts to control and prevent the transmission of malaria, which is part of the country’s prevention-based health policy.

Objective
This study aimed to determine malaria prevalence and associated risk factors among patients visiting Mizan-Tepi University Teaching Hospital (MTUTH).

Materials and methods
A cross-sectional study was conducted from September to December 2021 among patients visiting MTUTH, Southwest Ethiopia. A pretested structured questionnaire was used to collect sociodemographic data, and a capillary blood sample was collected after obtaining written informed consent from the study participants. The data were entered into Epi-data manager (v4.0.2.101) and analyzed with SPSS version 25.0, with a P-value of < 0.05 set as a significance.

Results
A total of 439 patients participated, of which 20.7% (91) were positive for malaria parasites, with a higher prevalence observed among the age group interval of 25–34 years (5.5%). Inadequate access to insecticide-treated bed net (ITN) 23.9% (105) and a low level of ITN usage 20.5% (90) were recorded. Patients living in areas of stagnant water were more likely to get infected with the malaria parasite (AOR = 16.191, 95% CI: 9.137, 28.692) compared to those who live away from stagnant water, and individuals living in houses not sprayed with insecticides were more susceptible to malaria infection (AOR = 0.215, 95% CI: 0.128, 0.360).

Conclusion
The overall malaria prevalence in this study was 20.7% (91), which proves that malaria remains a major threat to the communities in the study area, with Plasmodium falciparum contributing to most of the cases. Improving the habits of ITN usage and indoor residual spray through health education may help to reduce the impact of malaria in the study area.