bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D Printing: Applications in Tissue Engineering, Medical Devices, and Drug Delivery
Authors: Pavan Kalyan BG , Kumar Lalit 
Published: March 2022

AbstractThe gemstone of 3-dimensional (3D) printing shines up from the pyramid of additive manufacturing. Three-dimensional bioprinting technology has been predicted to be a game-changing breakthrough in the pharmaceutical industry since the last decade. It is fast evolving and finds its seats in a variety of domains, including aviation, defense, automobiles, replacement components, architecture, movies, musical instruments, forensic, dentistry, audiology, prosthetics, surgery, food, and fashion industry. In recent years, this miraculous manufacturing technology has become increasingly relevant for pharmaceutical purposes. Computer-aided drug (CAD) model will be developed by computer software and fed into bioprinters. Based on material inputs, the printers will recognize and produce the model scaffold. Techniques including stereolithography, selective laser sintering, selective laser melting, material extrusion, material jetting, inkjet-based, fused deposition modelling, binder deposition, and bioprinting expedite the printing process. Distinct advantages are rapid prototyping, flexible design, print on demand, light and strong parts, fast and cost-effective, and environment friendly. The present review gives a brief description of the conceptional 3-dimensional printing, followed by various techniques involved. A short note was explained about the fabricating materials in the pharmaceutical sector. The beam of light is thrown on the various applications in the pharma and medical arena.
Associations of Abdominal and Cardiovascular Adipose Tissue Depots With HDL Metrics in Midlife Women: the SWAN Study
Authors: Nasr Alexis , Matthews Karen , Janssen Imke , Brooks Maria M , Barinas-Mitchell Emma , Orchard Trevor J , Billheimer Jeffrey , Wang Norman C , McConnell Dan , Rader Daniel J , El Khoudary Samar R 
Published: March 2022

Abstract

Context
The menopause transition is accompanied by declines in the atheroprotective features of high-density lipoprotein (HDL), which are linked to deleterious cardiovascular (CV) outcomes.

Objective
This work aimed to assess the relationship between abdominal and CV visceral adipose tissues (VAT) with future HDL metrics in midlife women, and the role of insulin resistance (IR) on these associations.

Methods
Temporal associations compared abdominal and CV fat with later measures of HDL metrics. This community-based cohort comprised 299 women, baseline mean age 51.1 years (SD: 2.8 years), 67% White, 33% Black, from the Study of Women’s Health Across the Nation (SWAN) HDL ancillary study. Exposures included volumes of abdominal VAT, epicardial AT (EAT), paracardial AT (PAT), or perivascular AT (PVAT). Main outcomes included HDL cholesterol efflux capacity (HDL-CEC); HDL phospholipids (HDL-PL), triglycerides (HDL-Tgs), and cholesterol (HDL-C); apolipoprotein A-I (ApoA-I), and HDL particles (HDL-P) and size.

Results
In multivariable models, higher abdominal VAT was associated with lower HDL-CEC, HDL-PL, HDL-C, and large HDL-P and smaller HDL size. Higher PAT was associated with lower HDL-PL, HDL-C, and large HDL-P and smaller HDL size. Higher EAT was associated with higher small HDL-P. Higher PVAT volume was associated with lower HDL-CEC. The Homeostatic Model Assessment of Insulin Resistance partially mediated the associations between abdominal AT depots with HDL-CEC, HDL-C, large HDL-P, and HDL size; between PVAT with HDL-CEC; and PAT with HDL-C, large HDL-P, and HDL size.

Conclusion
In midlife women, higher VAT volumes predict HDL metrics 2 years later in life, possibly linking them to future CV disease. Managing IR may preclude the unfavorable effect of visceral fat on HDL metrics.
Coordinated activities of Myosin Vb isoforms and mTOR signaling regulate epithelial cell morphology during development
Authors: Gupta Kirti , Mukherjee Sudipta , Sen Sumit , Sonawane Mahendra 
Published: March 2022

ABSTRACT
The maintenance of epithelial architecture necessitates tight regulation of cell size and shape. However, mechanisms underlying epithelial cell size regulation remain poorly understood. We show that the interaction of Myosin Vb with Rab11 prevents the accumulation of apically derived endosomes to maintain cell-size, whereas that with Rab10 regulates vesicular transport from the trans-Golgi. These interactions are required for the fine-tuning of the epithelial cell morphology during zebrafish development. Furthermore, the compensatory cell growth upon cell-proliferation inhibition involves a preferential expansion of the apical domain, leading to flatter epithelial cells, an efficient strategy to cover the surface with fewer cells. This apical domain growth requires post-trans-Golgi transport mediated by the Rab10-interacting Myosin Vb isoform, downstream of the mTOR-Fatty Acid Synthase (FASN) axis. Changes in trans-Golgi morphology indicate that the Golgi synchronizes mTOR-FASN-regulated biosynthetic input and Myosin Vb-Rab10 dependent output. Our study unravels the mechanism of polarized growth in epithelial cells and delineates functions of Myosin Vb isoforms in cell size regulation during development.
MAPK activation drives male and female mouse teratocarcinomas from late primordial germ cells
Authors: Guida Eugenia , Tassinari Valentina , Colopi Ambra , Todaro Federica , Cesarini Valeriana , Jannini Benedetto , Pellegrini Manuela , Botti Flavia , Rossi Gabriele , Rossi Pellegrino , Jannini Emmanuele A. , Dolci Susanna 
Published: March 2022

ABSTRACT
Germ cell tumors (GCTs) are rare tumors that can develop in both sexes, peaking in adolescents. To understand the mechanisms that underlie germ cell transformation, we established a GCT mouse model carrying a germ-cell-specific BRafV600E mutation with or without heterozygous Pten deletion. Both male and female mice developed monolateral teratocarcinomas containing embryonal carcinoma (EC) cells that showed an aggressive phenotype and metastatic ability. Germ cell transformation started in fetal gonads and progressed after birth leading to gonadal invasion. Early postnatal testes showed foci of tumor transformation, whereas ovaries showed increased number of follicles, multi-ovular follicles (MOFs) and scattered metaphase I oocytes containing follicles. Our results indicate that MAPK (herein referring to Erk1/2) overactivation in fetal germ cells of both sexes can expand their proliferative window leading to neoplastic transformation and metastatic behavior.
The philanthropy's crucial role in alleviating stunting in Southeast Asian countries through research funding: a policy brief
Authors: Morasa Jenny , Nurkolis Fahrul , Narasiang Bezaliel R. , Visnu Jodi , Mayulu Nelly , Taslim Nurpudji Astuti , Gunawan William Ben , Handoko Matthew Nathaniel , Hardinsyah Hardinsyah 
Published: March 2022

Background: Developing countries need funding for researchers to maximize the evidence base, especially in stunting research. This short policy is expected to provide opportunities for Southeast Asian countries to receive more research funding to stunt philanthropists. Policy and implications: Multi-sector cooperation is arguably the leading player in acceleration programs to reduce growth delays. The Stunted Reduction Acceleration Program, complemented by higher research and development expenditures (RDEs) and open policies to support philanthropic activities in Indonesia, will help improve the efficiency of stunted stunt reduction through adequate funding and investment. As evidenced by Singapore and Thailand, the increase in government budgets focused on research and development (R&D) and supportive environments, as shown by Global Philanthropy Environment Index (GPEI), prove that evidence-based and practical researches can lead to the reduction of estimated prevalence of stunting rather than innovation-stage research. Recommendations: Attention should be paid to proposing major changes to the allocation of R&D expenses and creating an environment that supports philanthropy. The challenges associated with these issues are: (1) stunted research and innovation are still left behind. (2) The availability of data and the lack of transparency. (3) Subpar performance in relation to philanthropists. (4) Inconsistent legal definitions and a lack of industry-specific guidelines. (5) Inadequate law enforcement. (6) Local leaders must engage in evidence-based advocacy. (7) Philanthropists have few opportunities to learn.
Incidence and Risk Factors for Perioperative Cardiovascular Complications in Spine Surgery
Authors: Chotisukarat Haruthai , Akavipat Phuping , Suchartwatnachai Pathomporn , Sookplung Pimwan , Eiamcharoenwit Jatuporn 
Published: March 2022

Background: An increasing number of patients are opting for spine surgery despite the associated risk of cardiovascular complications. The evidence regarding the incidence and risk factors of cardiovascular complications in spine surgery is insufficient. Therefore, we aimed to determine the incidence and risk factors for cardiovascular complications that occur perioperatively in spine surgery. Methods: This retrospective study included all patients who underwent spine surgery between January 2018 and December 2019 at a single center. Demographic, clinical, and operative data were collected from electronic medical records. The incidence of perioperative cardiac complications was determined. Univariate and multivariate analyses were performed to identify risk factors for the development of perioperative cardiovascular complications in the participants. Results: Of the 1,002 eligible patients enrolled in the study, six developed cardiac complications. Acute myocardial infarction, cardiac arrest, and congestive heart failure occurred in one, two, and three patients, respectively. Risk factors for cardiovascular complications included scoliosis surgery (odds ratios (OR): 18.61; 95% confidence interval (CI): 1.346-257.35) and a history of congestive heart failure (OR: 120.97; 95% CI: 2.12-6898.80). Conclusion: The incidence of perioperative cardiovascular complications in patients who underwent spine surgery was 0.6%. High-risk patients should be closely monitored optimally managed throughout the perioperative period.
Controlling Epidemic Spread: Reducing Economic Losses with Targeted Closures
Authors: Birge John R. , Candogan Ozan , Feng Yiding 
Published: March 2022

Data on population movements can be helpful in designing targeted policy responses to curb epidemic spread. However, it is not clear how to exactly leverage such data and how valuable they might be for the control of epidemics. To explore these questions, we study a spatial epidemic model that explicitly accounts for population movements and propose an optimization framework for obtaining targeted policies that restrict economic activity in different neighborhoods of a city at different levels. We focus on COVID-19 and calibrate our model using the mobile phone data that capture individuals’ movements within New York City (NYC). We use these data to illustrate that targeting can allow for substantially higher employment levels than uniform (city-wide) policies when applied to reduce infections across a region of focus. In our NYC example (which focuses on the control of the disease in April 2020), our main model illustrates that appropriate targeting achieves a reduction in infections in all neighborhoods while resuming 23.1%–42.4% of the baseline nonteleworkable employment level. By contrast, uniform restriction policies that achieve the same policy goal permit 3.92–6.25 times less nonteleworkable employment. Our optimization framework demonstrates the potential of targeting to limit the economic costs of unemployment while curbing the spread of an epidemic. This paper was accepted by Carri Chan, healthcare management.
Development of a Dutch Training/Education Program for a Healthy Lifestyle of People With Intellectual Disability
Authors: Overwijk Annelies , Hilgenkamp Thessa I.M. , van der Schans Cees P. , van der Putten Annette A.J. , Waninge Aly 
Published: March 2022

Abstract
Individuals with intellectual disability (ID) need support from direct support professionals (DSPs) to engage in a healthy lifestyle. However, literature shows DSPs feel insufficiently equipped to support a healthy lifestyle. Therefore, the aim of this study is to develop a theory-based program for DSPs to support physical activity and healthy nutrition for people with moderate to profound levels of ID, and to design its evaluation. The Intervention Mapping Protocol (IM) was followed to develop a theory-based program for DSPs. The program evaluation consists of process and feasibility evaluations. This study provided a theory-based program consisting of a training and education section with online and face-to-face components to support DSPs in promoting health for people with ID.
Bragg grating sensor for refractive index based on a D-shaped circular photonic crystal fiber
Authors: Wei Na , Xu Piaorong , Yao Ye , Li Jianbo , Liu Exian , Luo Jianhua 
Published: March 2022

In this paper, a silica-based D-shaped circular photonic crystal fiber Bragg grating sensor for refractive index sensing is proposed theoretically. D-shaped fiber construction can effectively enhance the coupling effect between the guiding mode and external liquid analyte, which then causes a distinct shift in the typical reflection spectrum as the refractive index of the analyte varies. This design exhibits highly improved sensitivity of 487 nm/RIU in a large refractive index range from 1.30 to 1.40 compared with the previous fiber grating sensors. Study of the dependence of sensing performance on the structure parameters suggests that the resonance peak shifts towards longer wavelengths with the increased air-hole diameter of fiber, while it is almost immobile as the hole spacing and the number of air-hole layers change in a certain range. For the influence of the Bragg grating structure, results show that the resonance peak is not sensitive to the grating length, but linearly increases as the grating period expands. The effects of polishing depth and fiber preparation error on the sensor are also discussed in detail. This high-sensitivity sensor based on a D-shaped photonic crystal fiber and Bragg grating has great potential in biochemical detection, environmental monitoring, and medical sensing.
Outcomes of implementation of the FilmArray meningoencephalitis panel in a tertiary hospital between 2017 and 2020
Authors: Teoh TeeKeat , Powell James , O’Keeffe Jillian , Donlon Eoghan , Dillon Lisa , Lenihan Marie , Mostyn Amanda , Power Lorraine , Boers Peter , Stapleton Patrick J. , O’Connell Nuala H. , Dunne Colum P. 
Published: March 2022

Background
Acute meningoencephalitis is encountered commonly in the acute hospital setting and is associated with significant morbidity and mortality, in addition to significant healthcare costs. Multiplex PCR panels now allow syndromic testing for central nervous system infection. The BioFire® FilmArray® Meningoencephalitis (ME) allows testing of 14 target pathogens using only 0.2mls of cerebrospinal fluid (CSF). We conducted a retrospective observational study to assess the performance of the assay and secondarily to observe the clinical utility of negative results by comparing clinical outcomes of aseptic meningitis to bacterial and viral meningoencephalitis.

Methods
Data for CSF samples tested using the FilmArray ME panel from October 2017 to October 2020 were analysed. Detection of bacterial and viral targets was analysed. Admission to critical care area, 90-day readmission rates, average length of stay and 30-day and 90-day mortality were analysed for three groups with following diagnoses: bacterial meningitis, viral meningoencephalitis, or aseptic meningitis.

Results
From October 2017 to October 2020, 1926 CSF samples were received in the Clinical Microbiology laboratory. Of those, 543 CSF samples from 512 individual patients were tested using the FilmArray ME panel. Twenty-one bacterial targets and 56 viral targets were detected during the study period. For viral targets, the cumulative specificity was 98.9% (95% confidence interval: 93.1–99.9) when compared to the reference laboratory methods. The outcomes for 30- and 90-day mortality of the aseptic meningitis group were non-inferior relative to the viral meningoencephalitis and bacterial meningitis group. Patients with bacterial meningitis had a longer average length of stay. Aseptic meningitis was associated with a higher 90-day readmission rate than the other 2 groups, but without statistical significance.

Conclusion
In our hands, implementation of the FilmArray ME panel was relatively straightforward. We experienced a transition in our workflow processes that enabled streamlining of CSF diagnostics and the safe removal of Gram staining in those samples being tested by this molecular assay. Coupled to this improvement, there was a positive clinical impact on patient care due to rapid turnaround time to results.
The relation between urinary sodium and potassium excretion and risk of cardiovascular events and mortality in patients with cardiovascular disease
Authors: Groenland Eline H. , Vendeville Jean-Paul , Bots Michiel L. , de Borst Gert Jan , Nathoe Hendrik M. , Ruigrok Ynte M. , Blankestijn Peter J. , Visseren Frank L. J. , Spiering Wilko ,   on behalf of the UCC-SMART Study Group
Published: March 2022

Background
Most evidence on the relationship between sodium and potassium intake and cardiovascular disease originated from general population studies. This study aimed to evaluate the relation between estimated 24-hour sodium and potassium urinary excretion and the risk of recurrent vascular events and mortality in patients with vascular disease.

Methods
7561 patients with vascular disease enrolled in the UCC-SMART cohort (1996–2015) were included. Twenty-four hour sodium and potassium urinary excretion were estimated (Kawasaki formulae) from morning urine samples. Cox proportional hazard models with restricted cubic splines were used to evaluate the relation between estimated urinary salt excretion and major adverse cardiovascular events (MACE; including myocardial infarction, stroke, cardiovascular mortality) and all-cause mortality.

Results
After a median follow-up of 7.4 years (interquartile range: 4.1–11.0), the relations between estimated 24-hour sodium urinary excretion and outcomes were J-shaped with nadirs of 4.59 gram/day for recurrent MACE and 4.97 gram/day for all-cause mortality. The relation between sodium-to-potassium excretion ratio and outcomes were also J-shaped with nadirs of 2.71 for recurrent MACE and 2.60 for all-cause mortality. Higher potassium urinary excretion was related to an increased risk of both recurrent MACE (HR 1.25 per gram potassium excretion per day; 95%CI 1.13–1.39) and all cause-mortality (HR 1.13 per gram potassium excretion per day; 95%CI 1.03–1.25).

Conclusions
In patients with established vascular disease, lower and higher sodium intake were associated with higher risk of recurrent MACE and all-cause mortality. Higher estimated 24-hour potassium urinary excretion was associated with a higher risk of recurrent MACE and all-cause mortality.
Mental health follow-up and treatment engagement following suicide risk screening in the Veterans Health Administration
Authors: Bahraini Nazanin , Reis Daniel J. , Matarazzo Bridget B. , Hostetter Trisha , Wade Christina , Brenner Lisa A. 
Published: March 2022

Importance
Understanding the extent to which population-level suicide risk screening facilities follow-up and engagement in mental health treatment is important as engaging at-risk individuals in treatment is critical to reducing suicidal behaviors.

Objective
To evaluate mental health follow-up and treatment engagement in the Veterans Health Administration (VHA) following administration of the Columbia-Suicide Severity Rating Scale (C-SSRS) screen, a component of the VHA’s universal suicide risk screening program.

Design
This cross-sectional study used data from VA’s Corporate Data Warehouse.

Settings
140 VHA Medical Centers.

Participants
Patients who completed the C-SSRS screen in ambulatory care between October 1, 2018—September 30, 2020.

Exposure
Standardized suicide risk screening.

Main outcomes and measures
Mental health follow-up (one or more visits within 30 days of C-SSRS screening) and treatment engagement (two or more visits within 90 days of C-SSRS screening) were examined.

Results
97,224 Veterans in Fiscal Year 2019 (FY19) (mean age 51.4 years; 86.8% male; 64.8% white, 22.4% African-American) and 58,693 Veterans in FY20 (mean age 49.6 years; 85.5% male; 63.4% white, 21.9% African-American) received the C-SSRS screen. Across FYs, a positive C-SSRS screen was associated with increased probability of mental health follow-up and treatment engagement. Patients who were not seen in mental health in the year prior to screening had the greatest increase in probability of mental health follow-up and engagement following a positive screen (P<0.001). For FY19, a positive C-SSRS screen in non-mental health connected patients was associated with an increased probability of follow-up from 49.8% to 79.5% (relative risk = 1.60) and engagement from 39.5% to 63.6% (relative risk = 1.61). For mental health-connected patients, a positive C-SSRS screen was associated with a smaller increase in probability of follow-up from 75.8% to 87.6% (relative risk = 1.16) and engagement from 63.3% to 76.4% (relative risk = 1.21). Results for FY20 were similar.

Conclusions and relevance
Identification of suicide risk through population-level screening was associated with increased mental health follow-up and engagement, particularly for non-mental health connected patients. Findings support the use of a standardized, comprehensive suicide risk screening program for managing elevated suicide risk in a large healthcare system.
Use of oral polio vaccine and the incidence of COVID-19 in the world
Authors: Habibzadeh Farrokh , Chumakov Konstantin , Sajadi Mohammad M. , Yadollahie Mahboobeh , Stafford Kristen , Simi Ashraf , Kottilil Shyamasundaran , Hafizi-Rastani Iman , Gallo Robert C. 
Published: March 2022

Background
Several live attenuated vaccines were shown to provide temporary protection against a variety of infectious diseases through stimulation of the host innate immune system.

Objective
To test the hypothesis that countries using oral polio vaccine (OPV) have a lower cumulative number of cases diagnosed with COVID-19 per 100,000 population (CP100K) compared with those using only inactivated polio vaccine (IPV).

Methods
In an ecological study, the CP100K was compared between countries using OPV vs IPV. We used a random-effect meta-analysis technique to estimate the pooled mean for CP100K. We also used negative binomial regression with CP100K as the dependent variable and the human development index (HDI) and the type of vaccine used as independent variables.

Results
The pooled estimated mean CP100K was 4970 (95% CI 4030 to 5900) cases per 100,000 population for countries using IPV, significantly (p<0.001) higher than that for countries using OPV—1580 (1190 to 1960). Countries with higher HDI prefer to use IPV; those with lower HDI commonly use OPV. Both HDI and the type of vaccine were independent predictors of CP100K. Use of OPV compared to IPV could independently decrease the CP100K by an average of 30% at the mean HDI of 0.72.

Conclusions
Countries using OPV have a lower incidence of COVID-19 compared to those using IPV. This might suggest that OPV may either prevent SARS-CoV-2 infection at individual level or slow down the transmission at the community level.