bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying Student Mistreatment Through Peer-Facilitated Learning Environment Sessions
Authors: Erb Emily Cokorinos , Randolph Martine N. , Cohen-Osher Molly , Garg Priya S. 
Published: July 2022

Problem
Data from the Association of American Medical Colleges (AAMC) Medical School Graduation Questionnaire (GQ) show persistent high rates of medical student mistreatment, and multiple barriers to student reporting of mistreatment exist. The authors examined whether learning environment sessions (LESs) allow students opportunities to identify and describe patterns of mistreatment missed by other avenues of reporting.

Approach
Peer-facilitated LESs were instituted in 2018–2019 at Boston University School of Medicine. The LESs were scheduled once during every third-year core clerkship block. Third- and fourth-year students trained as peer-facilitators led discussions of topics relevant to the student clinical experience using a standardized facilitator guide. Minutes, including details of reported events, were completed during the session and visible to all students participating. These minutes were sent to clerkship leadership and the medical education office for action once student grades were submitted.

Outcomes
Summative content analysis was conducted on 44 LES minutes from sessions held in January–November 2019. Reported incidents were categorized into broad categories of negative treatment (NT), negative learning environment (NLE), and positive learning environment (PLE). Sixty-three instances of NT were identified. Of these, 37 fit within the scope of the AAMC GQ mistreatment categories. The remaining 26 instances of NT were classified into 7 novel categories of medical student mistreatment. Instances of NLE were most discussed by students and categorized into 5 subthemes. Examples of PLE were categorized into 4 subthemes, which encompassed 11 descriptors of core qualities of an ideal preceptor or educational environment.

Next Steps
LESs have aided in identifying and describing new patterns of mistreatment. They fulfill a unique role by allowing students to identify, analyze, and report mistreatment in a facilitated and protected space. Formal evaluations of institutional improvement in the learning environment, reduction in medical student mistreatment, and subsequent improvement in AAMC GQ data are needed.
Current status of phosphoinotiside-3 kinase inhibitors in blood cancers
Authors: Shouse Geoffrey , Danilova Olga V. , Danilov Alexey V. 
Published: July 2022

Purpose of review
Treatment of non-Hodgkin lymphoma (NHL) and chronic lymphocytic leukemia (CLL) underwent paradigm shifts, with targeted agents rapidly displacing chemotherapy. Phosphoinotiside-3 kinase (PI3K) is essential for survival and proliferation of neoplastic B cells and has proven a tractable target in NHL, with four agents receiving FDA approval in the last decade. This review summarizes key data and challenges associated with use of PI3K inhibitors in routine practice.

Recent findings
Idelalisib and duvelisib are active in CLL and indolent NHL, including in patients with high-risk features. Despite differential targeting of PI3K isoforms, they exhibit comparable efficacy and adverse event profile including autoimmune events (transaminitis, colitis, pneumonitis), mediated by Treg/Th17 imbalance. Although copanlisib, a pan-PI3K inhibitor, is associated with a distinct safety profile (hyperglycemia, hypertension), preclinical studies indicate that umbralisib, a dual inhibitor of PI3Kδ and casein kinase 1ε, may have less effect on Tregs. However, both drugs may still cause immune-mediated toxicities.

Summary
With close monitoring and management of adverse events, PI3K inhibitors continue to have a role in therapy of R/R CLL and NHL. Strategies to mitigate adverse events and increase efficacy of PI3K inhibitors include time-limited combination approaches, intermittent dosing schedules.
Is One Lecture Enough? Self-Perception of Bias and Cultural Training in Medical Education
Authors: Railey Kenyon , Barnett Jacqueline 
Published: July 2022

Introduction
A recognition of the importance of the sociocultural determinants of health and well-being has increased within medical education; yet there is variability in exposure and evidence of effect. The goal of this project was to assess contact with cultural humility or competency instruction prior to training, evaluate self-perception of bias, and test the effect of a single lecture on this perception.

Methods
A 17-item survey was administered to participants before and after a lecture “intervention.”

Results
Analysis revealed that 54% (n = 93) of participants received formal instruction prior to medical/graduate school. Subsequently, significant changes (p = 0.02) when comparing pretests, immediate posttests, and 8-week posttests were found in only one area, “ability to withhold judgment.” While the majority of participants reported having “bias towards certain groups” (chi-square p <0.0001), there was no statistically significant difference in improvement of self-reflection.

Discussion
A single lecture may improve personal awareness of bias but likely does not significantly affect reflection on this bias or improve self-perception of cultural competency.
Risk stratification and treatment of pulmonary embolism with intermediate-risk of mortality
Authors: Pastré Jean , Sanchis-Borja Mateo , Benlounes Manil 
Published: July 2022

Purpose of review
Intermediate-risk pulmonary embolisms (PE) represent a heterogeneous group at the high end of hemodynamically stable patients, characterized by a higher mortality rate. This challenging population gathers many unsolved question regarding its therapeutic management. The purpose of this review is to provide an updated overview of the literature regarding further risk stratification and treatment options in this population.

Recent findings
If anticoagulation represents the undisputed first line of treatment, some patients especially in the intermediate-high risk subgroup may necessitate or could benefit from therapeutic escalation with reperfusion therapies. This includes systemic thrombolysis (ST) or catheter-directed therapies (CDT). ST, despite its high efficacy, is not recommended in this population because of prohibitive bleeding complications. Therefore, reduced-dose ST appears to be a promising option and is actually under evaluation. CDT are percutaneous reperfusion techniques developed to acutely decrease pulmonary vascular obstruction with lower-dose or no thrombolytic agents and, thus, potentially improved safety compared to ST.

Summary
Great progress has been made in the recent years providing a wide range of therapeutic options. Optimal selection of patients who could benefit from these treatments is the key and is based on clinical, biological and radiological parameters evaluating right ventricle function and allowing accurate risk stratification. Pulmonary Embolism Response Team represents an efficient modality for therapeutic management especially in the intermediate-high risk subgroup.
Viability testing during liver preservation
Authors: Patrono Damiano , Lonati Caterina , Romagnoli Renato 
Published: July 2022

Purpose of review
Viability assessment is one of the main indications for machine perfusion (MP) in liver transplantation. This review summarizes the rationale, evolution and limitations of proposed viability criteria and suggests a framework for future studies.

Recent findings
Liver viability is most frequently assessed during normothermic MP by combining parameters relative to perfusate and bile composition, vascular flows and macroscopic aspect. Assessment protocols are largely heterogeneous and have significantly evolved over time, also within the same group, reflecting the ongoing evolution of the subject. Several recent preclinical studies using discarded human livers or animal models have explored other approaches to viability assessment. During hypothermic MP, perfusate flavin mononucleotide has emerged as a promising biomarker of mitochondrial injury and function. Most studies on the subject suffer from limitations, including low numbers, lack of multicenter validation, and subjective interpretation of some viability parameters.

Summary
MP adds a further element of complexity in the process of assessing the quality of a liver graft. Understanding the physiology of the parameters included in the different assessment protocols is necessary for their correct interpretation. Despite the possibility of assessing liver viability during MP, the importance of donor-recipient matching and operational variables should not be disregarded.
Internet use and internet-based addictive behaviours during coronavirus pandemic
Authors: Kovačić Petrović Zrnka , Peraica Tina , Kozarić-Kovačić Dragica , Palavra Irena Rojnić 
Published: July 2022

Purpose of review
The COVID-19 outbreak profoundly influenced human lives on different levels. This narrative review aims to present recent data relating to the COVID-19 pandemic and internet use and internet-based addictive behaviours [problematic Internet use (PIU), Internet addiction, Internet gaming disorder, problem gambling or gambling disorder, social media addiction including smartphone use disorder, problematic pornography use, and compulsive buying].

Recent findings
During the COVID-19 pandemic, the prevalence of Internet use and Internet-based addictive behaviours increased in all age groups. The COVID-19 pandemic and related containment measures were been associated with increased levels of stress, anxiety, and depression in the general population, especially among children and adolescents, which are the vulnerable groups for the development of mental disorders during the crisis. Furthermore, the fear resulting from the disease, the ‘lockdown’ measures, high levels of uncertainty regarding the future, and financial insecurity increased levels of loneliness, PIU, and Internet addiction.

Summary
Longitudinal researches focused on the negative impacts of Internet use in prolonged stress situations on mental health, vulnerable groups, and quality of life (QoL) are needed to help clinicians and policymakers to reduce adverse consequences. Prevention programs for Internet use and Internet-based addictive behaviours must be customized to meet the needs of children, adolescents, and adults. Also, treatment programs must be tailored to the needs of patients with different Internet-based addictions.
Interventions for attempted suicide
Authors: Menon Vikas , Vijayakumar Lakshmi 
Published: July 2022

Purpose of review
Suicide is a major, global, public health issue. Those who attempt suicide represent a high-risk subgroup for eventual death by suicide. We provide an update on emerging evidence for interventions for attempted suicide to reduce subsequent suicidal behavior.

Recent findings
Major approaches that have been examined recently include pharmacological, psychosocial, brief active contact and outreach interventions, and digitally driven interventions. Notwithstanding the limited evidence base for most of these approaches, brief contact and follow-up interventions appear to have more robust effects on reduction of repeat suicidal behavior, including attempts; such approaches may have especial significance in emergency settings because of their brevity. Digital interventions for self-harm appear promising in the short-term whereas the evidence for pharmacological and psychosocial strategies remain inconclusive.

Summary
Although current evidence supports the use of brief interventions, contact, and outreach for reducing risk of subsequent suicide attempts and suicidal behavior, there are large gaps and limitations in the evidence base related to trial design, lack of long-term efficacy data, and implementational challenges. More robustly designed long-term trials that examine integrated intervention approaches with well defined outcomes are needed to develop recommendations in this area.
An update on the epidemiology of eating disorders in Latin America: current findings and future challenges
Authors: Kolar David R. , Mebarak Moises 
Published: July 2022

Purpose of review
We systematically reviewed the recent literature on the epidemiology of eating disorders in Latin America.

Recent findings
Most screened articles only investigated risk for eating disorders or disordered eating behaviors. Four studies reported prevalence for eating disorders. One study reported age-standardized prevalence ranging from 0.04% [95% confidence interval, CI (0.03, 0.06)] to 0.09% [95% CI (0.07, 0.13)] for anorexia nervosa and from 0.13% [95% CI (0.08, 0.17)] to 0.27% [95% CI (0.18, 0.37)] for bulimia nervosa. Three additional studies conducted in Brazil identified a general eating disorder point-prevalence of 0.40% in children aged 6--14 years and a point-prevalence of 0.7 % [95% CI (0.34, 1.55)] for bulimia nervosa, 1.4% [95% CI (0.81, 2.43)] for binge-eating disorder and 6.2% [95% CI (3.10, 5.27)] for recurrent binge eating.

Summary
Since 2020, only few studies were published on the epidemiology of full-threshold eating disorders in Latin America. Prevalence was in a comparable range to previous findings. No studies regarding new DSM-5 eating disorder diagnoses were identified, and studies investigating the impact of the COVID-19 pandemic on prevalence or incidence of eating disorders in these countries are needed.
Systematic map of the most recent evidence (2010 to 2019) on ruminant production-limiting disease prevalence and associated mortality in Ethiopia
Authors: Tsouloufi Theodora K. , MacVicar Isla S. , Donnison Louise M. , Smyth Karen L. , Peters Andrew R. 
Published: July 2022

AbstractEthiopia’s livestock sector supports the livelihoods of millions of smallholder farmers. However, despite the improvements of recent years, livestock productivity remains low due to critical constraints, including infectious diseases. The aim of this study was to collate and synthesise the published evidence on ruminant disease frequency and disease-associated mortality in Ethiopia, by identifying knowledge gaps and clusters in the literature to provide the basis for a decision-making tool. Searches on both bibliographic and organisation databases were conducted in English and were restricted to the period 2010-2019. Search results were screened for relevance at title, abstract and full text level, which identified 716 articles relevant to the research question. The systematic map revealed an increased publication output from 2012-2017, compared to 2010-2011 and 2018-2019. Most studies were conducted in Oromia, Amhara and SNNPR. A substantial body of evidence was found for trypanosomosis, ectoparasite infestation, fasciolosis, nematodiasis, echinococcosis, brucellosis and bovine brucellosis. This study suggests that despite the high output of epidemiological publications, further understanding of a considerable number of diseases is required and where evidence is abundant, synthesis of information should be carried out in order to better inform decisions on disease control priorities in the livestock sector.
Sensory deprivation triggers phenotypic adjustment of dopaminergic interneurons in the mouse olfactory bulb
Authors: Alexandra Angelova , Marie-Catherine Tiveron , Mathieu Loizeau , Harold Cremer , Jean-Claude Platel 
Published: July 2022

abstractOlfactory sensory activity is a main factor factor controlling intergration and survival of neurons in the olfactory bulb. However, its impact on specific neuronal subtypes is unclear. Using reversible unilateral naris closure in concert with longitudinal in vivo imaging we show here that newborn GABAergic interneurons undergo significant cell death under sensory deprivation. In contrast, dopaminergic OB neurons survive under deprivation, but react with a reversible downregulation of tyrosine hydroxylase expression.long abstractNeurogenesis persists in the mammalian subventricular zone after birth, producing various populations of olfactory bulb (OB) interneurons. These include GABAergic and mixed dopaminergic/GABAergic double neurotransmitter neurons for the glomerular layer. While olfactory sensory activity is one of the main factors controlling newborn neuron integration, its effect on specific neuronal subtypes is far less clear. Here we use a reversible unilateral deprivation paradigm in combination with longitudinal in vivo imaging to characterize the behavior of newborn glomerular neurons. We find that a substantial fraction of purely GABAergic neurons die after four weeks of sensory deprivation. Tyrosine Hydroxylase (TH) positive dopaminergic/ GABAergic neurons show no signifficant cell death under deprivation, but react with an important decrease in TH expression levels. Importantly, this effect reverses after naris reopening, pointing to a specific adaptation of this neuron population to the level of sensory activity. We conclude that sensory deprivation induces adjustments in the excitation/inhibition balance of the OB implicating cell death and adaptation of neurotransmitter use in specific neuron types.
Activity-dependent stabilization of nascent dendritic spines requires non-enzymatic CaMKIIα function
Authors: Claiborne Nicole , Zito Karen 
Published: July 2022

ABSTRACTThe outgrowth and stabilization of nascent dendritic spines are crucial processes underlying learning and memory. Most new spines retract shortly after growth; only a small subset is stabilized and integrated into the new circuit connections that support learning. New spine stabilization has been shown to rely upon activity-dependent molecular mechanisms that also contribute to long-term potentiation (LTP) of synaptic strength. Indeed, disruption of the activity-dependent targeting of the kinase CaMKIIα to the GluN2B subunit of the NMDA-type glutamate receptor disrupts both LTP and activity-dependent stabilization of new spines. Yet it is not known which of CaMKIIα’s many enzymatic and structural functions are important for new spine stabilization. Here, we used two-photon imaging and photolysis of caged glutamate to monitor the activity-dependent stabilization of new dendritic spines on hippocampal CA1 neurons from mice of both sexes in conditions where CaMKIIα functional and structural interactions were altered. Surprisingly, we found that inhibiting CaMKIIα kinase activity either genetically or pharmacologically did not impair activity-dependent new spine stabilization. In contrast, shRNA knock-down of CaMKIIα abolished activity-dependent new spine stabilization, which was rescued by co-expressing shRNA-resistant CaMKIIα. Notably, overexpression of phospho-mimetic CaMKIIα-T286D, which exhibits activity-independent targeting to GluN2B, enhanced basal new spine survivorship in the absence of additional glutamatergic stimulation, even when kinase activity was disrupted. Together, our results suggest that nascent dendritic spine stabilization requires structural and scaffolding interactions mediated by CaMKIIα that are independent of its enzymatic activities.SIGNIFICANCE STATEMENTThe stabilization of nascent dendritic spines is thought to support lasting memory of learned experiences. Here, we show that scaffolding and structural interactions, but not the enzymatic activities, of the kinase CaMKIIα are required for activity-dependent new spine stabilization. This study furthers our understanding of the cellular and molecular processes that facilitate learning and memory in the mammalian brain. Understanding the cellular and molecular mechanisms of learning and memory is crucial for our ability to develop therapeutics for memory impairments associated with neurological and neurodegenerative disorders.
The contribution of sex chromosome conflict to disrupted spermatogenesis in hybrid house mice
Authors: Kopania Emily E. K. , Watson Eleanor M. , Rathje Claudia C. , Skinner Benjamin M. , Ellis Peter J. I. , Larson Erica L. , Good Jeffrey M. 
Published: July 2022

AbstractIncompatibilities on the sex chromosomes are important in the evolution of hybrid male sterility, but the evolutionary forces underlying this phenomenon are unclear. House mice (Mus musculus) lineages have provided powerful models for understanding the genetic basis of hybrid male sterility. X chromosome-autosome interactions cause strong incompatibilities inMus musculusF1 hybrids, but variation in sterility phenotypes suggests a more complex genetic basis. Additionally, XY chromosome conflict has resulted in rapid expansions of ampliconic genes with dosage-dependent expression that is essential to spermatogenesis. Here we evaluated the contribution of XY lineage mismatch to male fertility and stage-specific gene expression in hybrid mice. We performed backcrosses between two house mouse subspecies to generate reciprocal Y-introgression strains and used these strains to test the effects of XY mismatch in hybrids. Our transcriptome analyses of sorted spermatid cells revealed widespread overexpression of the X chromosome in sterile F1 hybrids independent of Y chromosome subspecies origin. Thus, postmeiotic overexpression of the X chromosome in sterile F1 mouse hybrids is likely a downstream consequence of disrupted meiotic X-inactivation rather than XY gene copy number imbalance. Y-chromosome introgression did result in subfertility phenotypes and disrupted expression of several autosomal genes in mice with an otherwise nonhybrid genomic background, suggesting that Y-linked incompatibilities contribute to reproductive barriers, but likely not as a direct consequence of XY conflict. Collectively, these findings suggest that rapid sex chromosome gene family evolution driven by genomic conflict has not resulted in strong male reproductive barriers between these subspecies of house mice.
Development of a versatile high-throughput mutagenesis assay with multiplexed short read NGS using DNA-barcoded supF shuttle vector library amplified in non-SOS E. coli
Authors: Kawai Hidehiko , Iwata Ren , Ebi Shungo , Sugihara Ryusei , Masuda Shogo , Fujiwara Chiho , Kimura Shingo , Kamiya Hiroyuki 
Published: July 2022

ABSTRACTA forward mutagenesis assay using the supF gene has been widely employed for the last several decades in studies addressing mutation frequencies and mutation spectra associated with various intrinsic and environmental mutagens. In this study, by using a supF shuttle vector and non-SOS-induced Escherichia coli with short read Next Generation Sequencing (NGS) technology, we present an advanced method for the study of mutations, which is simple, versatile, and cost-effective. We demonstrate the performance of our newly developed assay via pilot experiments with UV-irradiation, the results from which emerge more relevant than expected. The NGS data obtained from samples of the indicator E. coli grown on titer plates provides mutation frequency and spectrum data, and uncovers obscure mutations that cannot be detected by a conventional supF assay. Furthermore, a very small amount of NGS data from selection plates reveals the almost full spectrum of mutations in each specimen and offers us a novel insight into the mechanisms of mutagenesis, despite them being considered already well-known. We believe that the method presented here will contribute to future opportunities for research on mutagenesis, DNA repair, and cancer.