bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Diversity and Local Adaption of Alfalfa Populations (Medicago sativa L.) under Long-Term Grazing
Authors: Wang Hu , Coulman Bruce , Bai Yuguang , Tarˈan Bunyamin , Biligetu Bill 
Published: November 2022

Abstract
Genomic information on alfalfa adaptation to long-term grazing is useful for alfalfa genetic improvement. In this study, 14 alfalfa populations were collected from long-term grazing sites (> 25 yrs) across four soil zones in western Canada. Six agro-morphological and three nutritive value traits were evaluated from 2018 to 2020. The genotyping-by-sequencing (GBS) data of the alfalfa populations and environmental data were used for genotype-environment association (GEA). Alfalfa cultivars released between 1926–1980 were used to determine degree of genetic variation of the 14 populations. Both STRUCTURE and UPGMA based on 19,853 SNPs showed that the 14 alfalfa populations had varying levels of parentages from alfalfa sub-species Medicago sativa and M. falcata. The linear regression of STRUCTURE membership probability on phenotypic data indicated genetic variations of forage dry matter yield, spring vigor and plant height were low, but genetic variations of regrowth, fall plant height, days to flower and crude protein were still high for the 14 alfalfa populations. The GEA identified 31 SNPs associated with 13 candidate genes that were mainly associated with six environmental factors of the long-term grazing sites. Candidate genes underlying environmental factors were associated with a variety of proteins, which were involved in plant responses to abiotic stresses, i.e., drought, cold and salinity-alkali stresses.
Etiology and clinical characteristics of patients with severely impaired consciousness in prehospital settings: a retrospective study
Authors: Mizu Daisuke , Matsuoka Yoshinori , Nishida Haruka , Sakatani Tomoko , Teramoto Shoki , Ariyoshi Koichi 
Published: November 2022

Abstract
Background: Although patients with severely impaired consciousness require a rapid response, it is commonly difficult to obtain detailed histories and perform accurate physical examinations of these patients. Therefore, this study examined the causes of patients with severely impaired consciousness and the clinical characteristics in prehospital settings that are useful for differential diagnosis.
Methods: We conducted a single-center retrospective study using data from the Kobe City Fire Department database. We retrospectively examined patients aged ≥16 years with Japan Coma Scale III-digit codes during paramedic contact and transported them to our hospital between January 2018 and December 2018. Furthermore, we evaluated the age, systolic blood pressure (sBP), presence of airway obstruction, and mortality for each final diagnosis.
Results: Overall, 227 patients were included in this study. One hundred and twelve patients (49.3%) were male, and the median age was 71 years (interquartile range 50–83 years). Stroke was the most common cause (30%). In addition, intoxication and psychiatric disorders were significantly more common in younger patients (P < 0.01). sBP was the highest inpatients with stroke. Airway obstruction was found most frequently in patients with stroke (73.5%), with a significant difference compared with other differential diseases,except for environmental disorders and cardiovascular diseases (P < 0.01). Mortality was the highest in stroke (55.9%), which was significantly higher than that for other differential diagnoses, except for environmental disorders and cardiovascular diseases.Among the patients with stroke, 45.6% had ocular abnormalities.
Conclusion: Stroke was the most common cause of severely impaired consciousness. However, age, sBP, the presence of airway obstruction, and ocular abnormalities can be useful indicators for differential diagnosis in prehospital settings in patients with severe disturbance of consciousness.
Psychological capital and organizational citizenship behavior among nurses during the COVID-19 epidemic: Mediation of organizational commitment
Authors: Zeng Li , Feng fen , Jin Man , Li Xin , Peng Yihang , Wang Jialin 
Published: November 2022

Abstract
Background: Nurses' organizational citizenship behavior, a spontaneous "altruistic work behavior", may be affected by psychological capital and organizational commitment, but its mechanism is not clear. The aim of this study was to investigate the characteristics and distribution of psychological capital, organizational commitment and organizational citizenship behavior among nurses during the COVID-19 epidemic, and explore the mediating role of organizational commitment in psychological capital and organizational citizenship behavior.
Methods: Across-sectional survey was conducted among 746 nurses from 6 designated hospitals for treatment of COVID-19 in China. Descriptive statistics, Pearson correlation analysis, and structural equation model were used in this study.
Results: Nurses' psychological capital, organizational commitment and organizational citizenship behavior scores were 103.12 ± 15.57, 46.53 ± 7.14 and 101.47 ± 12.14, respectively. Additionally, organizational commitment partially mediates between psychological capital and organizational citizenship behavior.
Conclusions: Nurses' psychological capital, organizational commitment and organizational citizenship behavior were at the upper middle level during the COVID-19 epidemic, which was affected by social-demographic variables. Moreover, psychological capital can affect organizational citizenship behavior through the mediating effect of organizational commitment. The findings call for the nursing administrationshould develop and excavate nurses' psychological capital, enhance nurses' organizational commitment, so as to promote nurses' organizational citizenship behavior.
Effects of Uniform/Periodic Magnetic Fields on the Nonlinear Stability of Double Interfaces Between Three Magnetic Fluids Saturated in Porous Media
Authors: Moatimid Galal , Sayed Aya , Zekry Marwa 
Published: November 2022

Abstract
The current manuscript tackles the interaction between three viscous magnetic fluids placed on three layers and saturated in porous media. Two of them fill half the spaces above and below a thin layer that lies in the middle region. All layers are laterally extended to infinity in both horizontal directions. All fluids move in the same horizontal direction with different uniform velocities and are driven by pressure gradients. The system is stressed by tangential stationary/periodic magnetic fields. The viscous potential theory (VPT) is used to simplify the mathematical procedure. The motion of the fluids is described by the Brinkman-Darcy equations, and Maxwell equations are used for the magnetic field. The nonlinear technique is typically relying on solving linear equations of motion and presenting the nonlinear boundary conditions. The novelty of the problem concerns the nonlinear stability of the double interface under the impact of periodic magnetic fields. Therefore, the approach has resulted in two nonlinear characteristic differential equations governing the surface displacements. Accordingly, the development amplitudes of surface waves are designated by two nonlinear Schrödinger equations. Stability is theoretically analyzed; the nonlinear stability criterion is derived, and the corresponding nonlinear stability conditions are explored in detail. Approximate bounded solutions of the perturbed interfaces are estimated. Additionally, the thickness of the intermediate layer as a function of time is plotted. The impact of different parameters on the stability profile is investigated. For the middle layer, it is found that magnetic permeability, as well as viscosity, have a stabilizing effect. By contrast, the basic streaming, as well as permeability, have a destabilizing influence. The analysis of the periodic case shows that the lower interface is much more stable than the upper one. Engineering applications like petroleum products manufacturing and the electromagnetic field effect can be used to control the growth of the perturbation and then the recovery of crude oil from the pores of reservoir rocks.
Trophectoderm biopsy is associated with adverse obstetric outcomes rather than neonatal outcomes
Authors: Ji Hui , Zhang Mian-Qiu , Zhou Qiao , Zhang Song , Dong Li , Li Xiu-Ling , Zhao Chun , Ding Hui , Ling Xiu-Feng 
Published: November 2022

Abstract
Background: With the wide application of preimplantation genetic testing (PGT) with trophectoderm (TE) biopsy, the safety of PGT has always been a concern. Since TE subsequently forms the placenta, it is speculated that the removal of these cells was associated with adverse obstetrical or neonatal outcomes after single frozen-thawed blastocyst transfer (FBT). Previous studies report contradictory findings with respect to TE biopsy and obstetric and neonatal outcomes.
Methods: We conducted a retrospective cohort study including 720 patients with singleton pregnancies from single FBT cycles who delivered at the same university-affiliated hospital between January 2019 and March 2022. The cohorts were divided into two groups: the PGT group (blastocysts with TE biopsy, n=223) and the control group (blastocysts without biopsy, n=497). The PGT group was matched with the control group by propensity score matching (PSM) analysis at a ratio of 1:2. The enrolled sample sizes in the two groups were 215 and 385, respectively.
Results: Patient demographic characteristics were comparable between the groups after PSM. Patients in the PGT group had significantly higher rates of hypertensive disorders of pregnancy (HDP) (13.5 vs. 8.8%, adjusted odds ratio (aOR) 1.76, 95% confidence interval (CI) 1.02–3.05, P = 0.043), gestational hypertension (6.0 vs. 2.6%, aOR 2.65, 95% CI 1.12–6.30, P = 0.027) and abnormal umbilical cord (13.0 vs. 7.8%, aOR 1.83, 95% CI 1.05–3.17, P = 0.032). However, the occurrence of premature rupture of membranes (PROM) (12.1 vs. 19.7%, aOR 0.57, 95% CI 0.35–0.93, P = 0.025) was significantly lower in biopsied blastocysts than in unbiopsied embryos. There were no significant differences in regard to other obstetric and neonatal outcomes between the two groups.
Conclusions: Trophectoderm biopsy is a safe approach, as the neonatal outcomes from biopsied and unbiopsied embryos were comparable. Furthermore, PGT is associated with higher risks of HDP, gestational hypertension, and abnormal umbilical cord but has a protective effect on PROM.
Classification and applicable conditions of water rights systems
Authors: Yuanyuan Sun , Shaofeng Jia , Ru Jia 
Published: November 2022

Abstract
Water rights meta type is defined as the basic constitution element of water rights system, five water rights meta types are identified firstly based on the justice principles of water rights empowerment. Then it’s analyzed the hierarchical structure of water rights systems. The complex water rights systems in reality can be divided three levels - water rights meta type, unit water rights system and mixd water rights system. The water rights meta types are the most basic elements of water rights systems. And the unit water rights systems which are composed of water rights meta types are the smallest units that exist independently and are relatively stable and maintain the characteristics of the water rights system. While the mixed water rights systems are the mixed mosaic of different unit systems. Through collecting the data of influencing factors in research units, the threshold of influencing factors for each unit water rights system have been estimated. Meanwhile, it’s determined the applicable conditions of unit water rights systems quantitatively.
Comparisons of the safety and effectiveness of robot-assisted versus laparoscopic partial nephrectomy for central renal angiomyolipomas: A propensity score-matched analysis
Authors: Zhu Qi-Qi , Li Zhi-Wei , Liu Ying , Liu Yi-Fu , Zhang Zhi-Cheng , Gong Bin-Bin , Sun Ting 
Published: November 2022

Abstract
OBJECTIVE: To compare the safety and effectiveness of robot-assisted partial nephrectomy (RAPN) versus laparoscopic partial nephrectomy (LPN) in the treatment of central renal angiomyolipomas (AMLs).
METHODS: We retrospectively analyzed the clinical data of 103 patients who were treated with either RAPN or LPN for central renal angiomyolipomas between January 2017 and June 2022. Propensity scores were matched according to sex, age, laterality, body mass index (BMI), symptoms, diameter of tumor, location of tumor distribution, RENAL score, preoperative hemoglobin, preoperative serum creatinine, preoperative glomerular filtration rate (eGFR), chronic disease, previous abdominal surgery, preoperative SAE, ASA score, and duration of follow-up, and after matching, perioperative and prognostic data of the two groups were compared.
RESULTS: A total of 57 patients underwent RAPN, and 46 patients underwent LPN. Before matching, there were more complex AMLs in the RAPN group, and RENAL scores differed between the two groups (10 versus 9, P<0.001). After matching, the median warm ischemic time (WIT) in the RAPN group was significantly shorter than that in the LPN group (21.5 min versus 28 min, P=0.034), while the median operating time in the RAPN group was longer than that in the LPN group (190 min versus 160 min, P=0.02). The other indicators were not significantly different between the groups.
CONCLUSION: RAPN has a shorter warm ischemia time but a longer operating time for central renal angiomyolipomas. RAPN did not show superiority over LPN in terms of safety and effectiveness; it is safe and feasible to apply either surgical approach in the treatment of central renal angiomyolipomas.
Engaging of the mTOR signaling pathway by miR100 and miR101 in de novo acute myeloid leukemia
Authors: Kargar Maryam , Farsani Mehdi Allahbakhshian , Garavand Javad , Gorji Mahnaz , Rafie Mohammad , Mohammadi Mohammad Hossein 
Published: November 2022

Abstract
Background: MicroRNAs (miRs) affect carcinogenesis and tumor progression by changing numerous cellular functions. miR-100 and miR-101 have been presented to be aberrantly expressed in acute leukemia. This study focused on their expression level in acute myeloid leukemia and their possible targets in molecular networks.
Methods:miR-100 and miR-101 expression were analyzed in 21 patients with acute myeloid leukemia compared to 9 healthy controls by using quantitative RT-PCR in mononuclear cells of peripheral blood and bone marrow samples. The target of miR-100 and miR-101 were predicted with TargetScan,miRDB, and miRanda. Finally, the relationship of these microRNAs with mTOR/AKT/PI3Ksignaling pathway genes was investigated.
Results: In this study, miR-100 was up-regulated (P value: 0.033; fold change: 6.8) whereas miR-101 (P value: 0.019; fold change : 0.61), mTOR (P value: 0.004; fold change: 0.56 ) and PI3KCA (P value <0.0001; fold change : 0.25) was downregulated in AML patients; there was a negative and positive correlation between the expression of miR-100 (r = -0.39, P value: 0.041) and miR-101 (r = 0.41, P value: 0.029) with the mTOR gene, while no meaningful correlation was seen between these miRs and the AKT1 and PI3KCAgenes.
Conclusion: These data showed tumor suppressor role for both miR-100 and miR-101 via mTOR/AKT/PI3K signaling pathway, therefore it can be a favorable therapeutic target beside other ones. More investigation of the miR-100 and miR-101 network with other signaling pathways in AML is recommended.
Expression pattern of Autophagy-related Genes and Immune Infiltration Landscape in Thoracic Aortic Aneurysm
Authors: Zhu Haoyan , Wu Jiahe , Li Chenze , Hu Yushuang , Zhang Tong , Lei Zhe , Wang Qiongxin , Cai Huanhuan , Lu Zhibing 
Published: November 2022

Abstract
Thoracic aortic aneurysm (TAA), a life-threatening cardiovascular disease, is considered a common cause of death in older individuals. Studies on the molecular mechanism of TAA are very limited. The aim of this study is to explore the expression pattern of autophagy-related genes (ARGs) in TAA, and the related immune infiltration landscape, using bioinformatics analysis. Differentially expressed genes (DEGs) in GSE26155 and differentially expressed miRNAs in GSE110527 were screened by R software. GO annotation and KEGG pathway enrichment analysis were performed on these DEGs. STRING database was employed for the PPI network establishment which was visualized by Cytoscape software. The differentially expressed ARGs were obtained by merging these DEGs with ARGs in autophagy databases HAMDb and HADb. The expression patterns of these differentially expressed ARGs are shown by violin diagram, correlation heatmap, PPI network and enrichment analysis. ROC analysis was used to evaluated the diagnostic value of these differentially expressed ARGs in TAA. Genes with area under the curve (AUC) > 0.7 were considered as TAA biomarkers. TargetScan, miRDB and miRWalk databases were used to predict miRNAs that target these ARGs. Intersection of predicted miRNAs and differentially expressed miRNAs to screen miRNAs that have a clear regulatory relationship with genes. Then a miRNA-mRNA regulatory network was constructed. Following this, the immune infiltration landscape of TAA and the correlation between these ARGs and immunity were further analyzed. In this study, 15 differentially expressed ARGs were screened and the expression patterns of these ARGs were investigated. A miRNA-mRNA regulatory network was constructed. 13 genes with AUC>0.7 in these differentially expressed ARGs were recognized as TAA biomarkers. Finally, the immune infiltration landscape of TAA was shown.
The Relationship between Social Constrains, Social Isolation, Family Environment, Self-Efficacy, and Depressive Symptoms among Breast Cancer Patients in China
Authors: Gu ZhiHui , Li MengYao , Liu Li , Ban Yue , Wu Hui 
Published: November 2022

Abstract
Background: For female breast cancer patients, the psychological status after surgery, especially the social and family psychological related factors, deserves more attention. This study analyzed the influence of social constrain, social support, social isolation, family conflict, family emotion expression on depression. At the same time, this study conducted the relationship between the variables and the mechanism of action.
Methods: 522 breast cancer patients finished questionnaires consisting of Self-Rating Depression Scale (SDS), General Self-Efficacy Scale (GSES), Multi-Dimensional Scale of Perceived Social Support (MSPSS), Social Constraints Scale-5(SCS-5), Family Environment Scale(FES), and Lubben Social Network Scale (LSNS-6). Multivariable Logical Regression was used to explore influencing factors. Pearson’s Correlation, Hierarchical Regression and Simple Slope Analysis were conducted to verify the role of self-efficacy.
Results: 71.6% of patients had depressive symptoms. Family contradiction (OR=10.086), social constrain (OR=2.522), social isolation (OR=2.507), and high blood glucose (OR=2.156) were risk factors of depressive symptoms. Family emotional expression (OR=0.480), family intimacy (OR=0.235), and self-efficacy (OR=0.246) were protective factors against depressive symptoms. The interactive items interpretation quantity was as follows: Contradiction*Self-efficacy (ΔR2=2.3%, P<0.001), Emotional expression*Self-efficacy (ΔR2=2.6%, P<0.001), Intimacy*Self-efficacy (ΔR2=1.0%, P=0.018), Social constrain*Self-efficacy (ΔR2=1.0%, P=0.008), Social networks*Self-efficacy (ΔR2=1.0%, P=0.010), Blood Glucose*Self-efficacy (ΔR2=0.6%, P=0.023). The influence of independent variables on depressive symptoms were gradually decreased in the low, mean and high groups of self-efficacy.
Conclusion: Postoperative Chinese breast cancer survivors reported higher depressive symptoms. Social, family, and physiological factors can affect depressive symptoms, in which self-factor played moderator roles.
Comparison of Chemical comstituents and content analysis in different parts of Eucommia ulmoides based on UPLC-Q-Orbitrap-MS and RP-HPLC
Authors: Gong Pin , Zhai Pengtao , Chen Xuefeng , Sun Wangyang , Wang Peipei , Guo Yuxi , Wang Zhineng , Li Nan , Zhai Wenjun , Zheng Benzhong 
Published: November 2022

Abstract
Eucommia ulmoides is a valuable tonic Chinese herbal medicine with a long history. It has the effects of tonifying liver and kidney, strengthening muscles and bones, regulating Chong and Ren channels, consolidating meridians and preventing miscarriage. Modern pharmacological research has demonstrated that Eucommia ulmoides has the pharmacological effects of lowering blood lipids, lowering blood pressure, lowering blood sugar, preventing aging, and inhibiting oxidation. Currently, only the bark of Eucommia ulmoides can be utilized as a therapeutic component; the plant's overall consumption is still quite limited. The growth of Eucommia ulmoides bark is extremely sluggish, typically taking more than 12 years, and the greater technical needs of Eucommia ulmoides bark can easily result in the death of the tree, which is not favorable to the sustainable use of plant resources. In order to identify raw materials that can substitute Eucommia ulmoides bark, this experiment investigated the active components of different position of Eucommia ulmoides. Through ultraperformance liquid chromatography-quadrupole tandem mass spectrometry, the chemical constituents of several Eucommia ulmoides parts were examined in this work (UPLC-Q-Orbitrap-MS). The identification of 119 compounds of twelve known and novel kinds. In this investigation, a high performance liquid chromatography (HPLC) method for analyzing the quantity of active components in various Eucommia ulmoides parts was developed. The results showed that there were some differences in the components and contents of different parts of Eucommia ulmoides. The bark of Eucommia ulmoides included higher levels of geniposidic acid, pinoresinol diglucoside, and quercetin, whereas the leaves contained higher levels of chlorogenic acid, catechin, and rutin. The bark and Eucommia ulmoides had the highest concentrations of active components, followed by the remaining sections. Therefore, Eucommia ulmoides leaves can replace Eucommia ulmoides bark for anti-oxidation, anti-inflammatory, antibacterial, and glucose and lipid metabolism regulation. This research presents a novel method for evaluating the quality of Eucommia ulmoides.