bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fast stitching method for multi-view images of cupping spots
Authors: Liu Ying-Bin , Qin Jian-Hua , Zhu Meng-Yan , Huang Ting-Ting 
Published: October 2022

Abstract
Aiming at the problems of cupping machine camera with a small field of view, complete and high-definition cupping spot characteristics need to be monitored in real-time, so as to effectively ensure that the cupping machine can accurately control the cupping time to avoid harm to the human body and extracting the complete back contour for automatic acupuncture point positioning, a fast stitching method for multi-view image of cupping spots is proposed. The present study uses linear transformation and Gaussian smoothing to preprocess the images to enhance image details, improve contrast, and reduce the influence of tank occlusion; What’s more, combing the BRISK algorithm and matching factor to match the image and estimate the overlapping area through establishing a binary tree model so as to improve the efficiency of feature point detection and matching. Experimental results show that compared with the Autostitch, the image stitched which is high definition has no obvious seams, ghosting, and distortion. What’s more, the algorithm in this paper is more efficient and the back contour is more complete.
The algorithm in this paper has good real-time performance and the clarity of the cupping spots is high, which is conducive to the subsequent real-time detection of the cupping spots. At the same time, the back contour of the image stitched by the algorithm in this paper is complete, which is conducive to the subsequent automatic acupuncture point positioning.
European Championship 2020 / 2021: Analysis of Goals Scored
Authors: Zivanovic Vladimir 
Published: October 2022

Abstract
Background Scoring of goals determines whether a team wins or loses a game. This is because the object of the game is to score goals even as you strive to stop your opponent from scoring.
Aims The aims to analyze the characteristics of the goals scored at the tournament, and derive the latest tactical innovations.
Methods In a sample of 51 games and the participation of 24 national teams, this study was based on the personal observation of the researcher. Two-way between-groups ANOVA were used for the data.
Results During the competition were scored 142 goals (2.79 per match). The fixed factor(a) showed the highest representation of goals was in the period of 45-60 minutes (30), while the fixed factors(b) included range of (65) for 2-3 goals (52.5 minutes) per game. Attack types and the number of goals scored was as follows: (80) continuous attacks (53.7 minutes), (13) quick attacks (59.5 minutes), (14) counter-attacks (55.7 minutes), and (35) set plays (51.8 minutes). The highest number set play of goals (13) was yielded following a corners (49.0 minutes) as compared to (9) penalties (60.0), and (8) free kicks non-directly (46.5 minutes). The actions that occurred prior to the goal, (45) 1, 2, 3 passes (54.0 minutes), and (32) long pass (53.0) were processed. The most of the goals (90) were achieved through the penalty area (54.1 minutes).
Conclusions The results reveal that coaches should the most focus on quick attacks, and counter-attacks which consequently leads to goal scoring.
Echinacea angustifolia encapsulated with alginate/chitosan nanoparticles as a novel candidate carrier for combating multidrug-resistant Staphylococcus aureus
Authors: Taghiloo Sepideh , Piri‐Gharaghie Tohid , Zand Zahra , Kabiri-Samani Saber , Kabiri Hamidreza , Rajaei Negin 
Published: October 2022

Abstract
Background and aim:
The urgent need for breakthrough therapeutic approaches for multi-drug resistance Staphylococcus aureus (MDR) has drawn attention to potential cutting-edge tools like nanoparticles (NPs). The preparation of chitosan/alginate-encapsulated Echinacea angustifolia extract and evaluation of its effectiveness against MDR strains were the goals of this investigation.
Method
Scanning electron microscopy, dynamic light scattering (DLS), and Fourier Transform Infrared spectroscopy were used to analyze the synthesized NPs. Investigations were done into the antibacterial and antibiofilm properties of E. angustifolia NPs. Also, the expression of essential genes in biofilm formation was evaluated by real-time PCR.
Results
According to DLS measurements, spherical E. angustifolia NPs had a diameter of 335.3 ± 1.43 nm. The PDI was 0.681, and the entrapment effectiveness (EE%) of the E. angustifolia extract reached 83.45%. Synthesized NPs had the highest antibacterial power. They also significantly decreased the expression of genes involved in biofilm formation.
Conclusion
By releasing natural-derived medications under regulated conditions, the findings described here should help create new plant extracts with increased stability and antibacterial action, which will help minimize the usage of antibiotics.
Identification of the WRKY gene family and the functional analysis of DoWRKY71 in yam (Dioscorea opposita Thunb.)
Authors: Xing Linan , Zhang Yanfang , Ge Mingran , Zhao Lingmin , Huo Xiuwen 
Published: October 2022

Abstract
Background:Dioscorea opposita Thunb. is one of the top 10 most important edible tuber and root plants worldwide, have been widely cultivated for nutritional and medicinal purposes. Yam is susceptible to various biological and abiotic stresses that will affect the yield and quality of the tubers. The WRKY gene family are widely distributed in plants growth and respond to abiotic stresses. However, current information on WRKY characteristics in yam is limited.
Results: This study identifed 22 DoWRKYgenes from transcriptome of Dioscorea spp., which were divided into 6 clades based on phylogenetic analysis. Analysis of conserved motifs showed that there were 10 conserved motifs, with lengths ranging between 17 and 50 amino acids. Expression pattern analysis showed DoWRKYgenes are expressed in different growth and development stages and responsed to a variety of abiotic stress. DoWRKY71 was up-regulated in the late stage of tuber expansion, and it was differentially expressed in cold and ABA stress. Therefore, further study indicated that DoWRKY71 protein was localized in the nucleus. Overexpression of DoWRKY71 enhanced the cold tolerance of transgenic tobacco, promoted abscisic acid-mediated stomatal closure.
Conclusions: This paper is the first systematic analysis the WRKY gene family in Dioscorea spp., and provide a new insight for further studying WRKY transcription factors in Dioscorea spp.. The functional study of DoWRKY71 laid a theoretical foundation for further exploring the regulatory function of DoWRKY71 gene in the growth and development related signaling pathway of yam.
LINC02454 related key pathways and genes in papillary thyroid cancer by weighted gene co-expression network analysis (WGCNA)
Authors: Song Yingjian , Ren Yi , Zhou Xilei , Tan Juan 
Published: October 2022

Abstract
Our previous study has demonstrated that long intergenic non-coding RNA 02454 (LINC02454) may act as an oncogene to promote proliferation and inhibit apoptosis in papillary thyroid cancer (PTC) cells. This study was designed to investigate the mechanisms whereby LINC02454 relates to PTC tumorigenesis.The thyroid cancer RNA sequence data were obtained from The Cancer Genome Atlas database. Weighted gene co-expression network analysis (WGCNA) was applied to identify LINC02454 modules associated with PTC. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was used to identify the key pathways and MCC topological method was used to identify the hub genes. Gene Expression Profiling Interactive Analysis (GEPIA) database was used to analyze different expression levels of hub genes between PTC samples and normal samples and explore the prognostic value of hub genes. A total of top 5000 variable genes were investigated, followed by an analysis of 8 modules, and the turquoise module was the most positively correlated with the clinical stage of PTC. KEGG pathway analysis demonstrated the top two pathways of the ECM − receptor interaction and MAPK signaling pathway. In addition, five hub genes (namely FN1, LAMB3, ITGA3, SDC4, and IL1RAP) were identified based on MCC algorithm and KEGG analysis. Further, the expression levels of the five hub genes were significantly upregulated in thyroid cancer, of these five genes, two hub genes (FN1 and ITGA3) were associated with poor disease-free prognosis.Our study identified five hub genes and two key pathways associated with LINC02454, which might disclose the underlying mechanism of LINC02454 action in PTC.
Management and physical exercise for spinal cord injury: a systematic review
Authors: Feria-Madueño Adrián , Pascua Daniel , Muñoz-Moyano Carlos 
Published: October 2022

Abstract
Purpose
Identify and analyse the scientific evidence about the variables that regulate the adaptation to physical exercise among people with spinal cord injury and the potential effects of new technologies in terms of injuries management. More specifically, relate each of them in order to preserve the integrity of these people in sport and improve their living conditions.
Methods
A systematic review is carried out in different databases through the Boolean method: Pubmed, Scopus and WOS. Some of these studies are collected between the last 22 years (1998–2020) and present a high degree of evidence.
Results
The 7 research works collected are heterogeneous in related to type of intervention and content. An analysis of the impact factor is carried out and its main data is synthesized. The majority focuses on cardiovascular adaptations and the regulation of the autonomic. nervous system.
Conclusion
Proper knowledge of the degree of autonomic dysfunction can significantly improve the likelihood that individuals decrease the risk of alteration in the regulation of blood pressure. Therefore, it ́s development should prevent the appearance of other diseases in those who have more chances to develop them.
A Qualitative Exploration of Young Australians' Experiences of Social Media's Impacts on Relationship Development
Authors: Cooper Spring Chenoa , Ferreira Kateryn , Edwards Raz G. , Keegan Julia , Norvila Nika , Lewis Larissa , Albury Kath , Skinner S. Rachel 
Published: October 2022

Abstract
Introduction. The present investigation is part of the Social Networks and Agency Project (SNAP), an 18-month longitudinal mixed methods study following adolescents located in New South Wales, Australia. The SNAP study aims to understand how online and offline social networks impact the development of sexual agency over time.
Methods. This paper presents an analysis of the second interview time point in the SNAP study. Fifty adolescents (aged 14-17) completed a semi-structured interview either face-to-face or via Skype about their romantic relationships, including where they get their sexual health information and how they flirt (both online and offline). Interviews were transcribed and analyzed using a grounded theory approach.
Results. The core theme was: Young people clearly demonstrate emerging sexual agency. The three characterizing themes were: 1) Young people have complex schemas about flirting, dating, and entering romantic and sexual relationships; 2) Young people have high literacy in social media, traditional media and pornography; and 3) Peers influence most aspects of developing sexual agency, including sex and relationship attitudes.
Discussion. We discuss possible methods to utilize adolescents’ social networks in facilitating the development of sexual agency.
Floristic diversity of native and non-native plants along the roadside of Tehsil Khadukhel, District Buner, Northern Pakistan
Authors: Jan Hammad , Haq Shiekh Marifatul , Wali Sher , Rahim Fazli , Ali Sajjad , Khalid Shah 
Published: October 2022

Abstract

Invasive plants that can withstand disturbances typically predominate in the roadside vegetation. The current study was designed to examine the patterns of floristic diversity along the roadside in Tehsil Khanudkhel, District Buner. Raunkiaer's classification was used to classify the plants that were gathered. All of the plants collected were divided into two groups: native and non-native. The field study was conducted from 2020-21, and 258 plant species from 82 families were collected. There were 108 non-native plants and 150 native plants. Family Asteraceae was the dominant one with 28 representative species. According to Rankuier's classification of living forms, the collected plants were divided into ten separate groups. Therophytes, which have 69 species, were the most prevalent life form. Seven groups of plants were created based on Rankuier's classification of leaf size. Microphyllous was the most prevalent leaf size form, with 112 species. Our study shows that the one disturbance event related to building roads in this Himalayan region had significant and long-lasting effects on plant communities at and around this transport corridor, suggesting the need for a long-term coordinated monitoring and management program for the entire region. This study also provides baseline data that is relevant to policy and can assist urban planners in developing sustainable planning.
Environmental risk assessment of watercourses and streambed sediments downstream the Capillitas Mine, Catamarca, Argentina
Authors: CAMPO Margarita DO , VALENZUELA María Fernanda , FERRO Leonardo 
Published: October 2022

Abstract

The present study assessed the environmental risk associated with the dispersion of heavy metals and metalloids by streams that flow through Capillitas Mine (Catamarca, Argentina), an epithermal precious- and base-metal vein deposit previously mined for copper along with gold and silver; and subsequently for rodhochrosite. Geochemical analyses of water of three streams (Ortíz, Acid and Capillitas) and a small pound depicting a very thin layer of turquoise-colored precipitates in its bottom were performed and the environmental risk factor was calculated. Moreover, the concentrations of potential toxic elements in sediments from the Ortíz and Capillitas streams were determined by ICP-OES and the Index of geoacumulation (Igeo) for As, Cd, Zn, Cu, Mn and Pb was computed. According to their major ions concentration the four water samples correspond to calcium SO4 waters. The Acid and Ortíz streams and the turquoise pound show concentrations of Al, Mn, Cd, Zn, Cu, Cr, Ni and Pb that largely exceed the limiting concentrations for protection of aquatic life, as well as for irrigation waters. The Capillitas stream has a lower content of heavy metal but shows concentrations of Mn, Zn, Cu and Pb exceeding the limiting values for protection of aquatic life. According to the obtained Igeo the three streambed sediments from the Ortíz creek are extremely polluted for Cd, Zn and Pb, strongly to extremely polluted for Cu and strongly to extremely polluted for As and Mn. The sediments from Capillitas creek depict lower contents of metals and metalloids; though it is strongly polluted for Cd, moderately polluted for Cu and unpolluted to moderately polluted for Pb and Zn.
IL-19 may aggravate atopic dermatitis by enhancing the role of IL-4/IL-13 in downregulating keratinocyte barrier proteins and upregulating the secretion of proinflammatory cytokines
Authors: Liu Lu , Wang Li , Ge Lan , Wang Huan , Feng Shujing , Jiang Lanlan , Jiang Pengju , Song Zhiqiang 
Published: October 2022

AbstractAtopic dermatitis (AD) is a relapsing inflammatory skin disorder, with characteristic T helper 2 (Th2)-based immune dysregulation and epidermal barrier function defect. In AD, keratinocyte plays a critical role in maintaining skin integrity and immune homeostasis. Interleukin (IL)-19 is a member of the IL-10 cytokine family. It can be secreted by and act on keratinocytes. Although it has been demonstrated that increased IL-19 in AD patients’ lesions and serum is positively correlated with the severity of AD, the role of IL-19 in the regulation of the epidermal barrier and immune homeostasis of keratinocytes remains unclear. Thus, we aim to investigate the effect of IL-19 on the production of epidermal barrier related proteins and proinflammatory cytokines in keratinocytes, and the synergistic effect of IL-19 with IL-4/IL-13in vitroexperiment. In this study, barrier related proteins (filaggrin/FLG, loricrin/LOR, keratin-10), epithelium-derived cytokines (thymic stromal lymphopoietin/TSLP, IL-33, IL-25), IL-19 and the phosphorylation level of STAT3 and STAT6 were measured in HaCaT cells by RT-PCR and/or western blot before and after stimulated with IL-4/IL-13 with or without different concentrations of IL-19. The changes of IL-19 levels in HaCaT cells after stimulation with house dust mite (HDM) or staphylococcal enterotoxin type B (SEB) were also examined. We found that IL-19 could promote the production of TSLP in keratinocytes, but reduce the expression levels of LOR and keratin-10. Meanwhile, IL-19 significantly enhanced the effects of IL-4/IL-13 on keratinocytes, including inducing the expression of IL-19 and epithelial derived cytokines and inhibiting the expression of barrier proteins. These enhanced effects were accompanied by increased phosphorylation of STAT3 and STAT6, but no change in the expression level of IL-4/13 receptor. In addition, HDM but not SEB can induce keratinocytes to secrete IL-19. Taken together, IL-19 can enhance the effect of IL-4 / IL-13 on keratinocytesin vitro, and may play an important role in the pathogenesis and progression of AD.
Prediction of vascular events in individuals with atherosclerotic lesions of various vascular basins: results of a 3-year prospective follow-up
Authors: Kobzeva N. D. , Terentev V. P. , Oleynikova V. D. 
Published: October 2022

Introduction. Today, cardiovascular diseases occupy a leading position in the structure of mortality, both in Russia and throughout the world. Every year more than 17 million people die from cardiac pathology, according to the World Health Organization, an increase in morbidity and mortality is expected in the future, which is explained, first of all, by an increase in the number of patients with diabetes and obesity.Aim. Development of optimal approaches to predicting cardiovascular events and unfavorable outcomes in patients with peripheral atherosclerosis during a 3-year prospective follow-up.Materials and methods. The study included 519 patients with atherosclerotic lesions of various vascular regions, of which 360 (69.4%) were men, 159 (30.6%) were women. The average age of the examined patients was 60.0 ± 8.7 years.Results. In the course of the analysis, it was noted that a factor such as the percentage of table stenosis of the left coronary artery (p = 0.013) influenced the risk of developing non-fatal cases of heart failure in patients with peripheral atherosclerosis. With regard to the end point – fatal cases of heart failure, the following factors demonstrated their statistical significance: age of onset of arterial hypertension, years (p = 0.020); history of chronic heart failure (p = 0.020); left atrial size, mm (p = 0.025); degree of stenosis of the posterior lateral branch, % (p = 0.038); presence of atherosclerotic lesions in the region of the posterior interventricular branch (p = 0.002); number of affected vascular beds (p = 0.044).Conclusions. Using logistic regression equations, original mathematical tools have been developed to assess the risk of developing both fatal and non-fatal heart failure in patients with peripheral atherosclerosis.
Association between adipokines and cardiac remodeling in obese patients in preclinical heart failure
Authors: Lyasnikova E. A. , Matveev G. A. , Golikova T. I. , Derevitskii I. V. , Fedorov A. V. , Vasilyeva E. Yu. , Babenko A. Yu. 
Published: October 2022

Introduction. Association of left ventricular hypertrophy (LVH) in obesity and accompanying metabolic risks with adipokines levels at the different stage of heart failure (HF) is still debatable.The aim of study was to investigate the relationship of circulating adipokines levels with LVH in obese patients at preclinical stage of HF.Materials and methods. The study included 74 obese patients: 43% had no markers of LVH (stage A HF, group 1); 57% had LVH (stage B HF, group 2). Transthoracic echocardiography, laboratory assessment of N-terminal fragment of the brain natriuretic peptide, soluble suppression of tumorigenesis-2 (sST2), circulating leptin and adiponectin levels, homeostasis model assessment of insulin resistance (IR) (HOMA-IR) were done. Matched-pairs analysis was applied.Results. Negative correlations of LVH with leptin levels in group 1 (stage A HF) and with adiponectin levels in group 2 (stage B HF) were detected (all p < 0.05). Positive correlations of the sST2 / adiponectin ratio and HOMA-IR with the parameters of LVH were detected in group 2 (all p >< 0.05). Conclusion. The direction of the associations between circulating adipokines and LVH varies with the preclinical stage of HF. The data obtained may reflect a relationship between heart remodeling in response to molecular mechanisms of inflammation and IR in obese patients at the certain stage of cardiovascular continuum. Keywords: leptin, adiponectin, insulin resistance, HOMA-IR, inflammation, sST2, left ventricular hypertrophy>˂ 0.05). Positive correlations of the sST2 / adiponectin ratio and HOMA-IR with the parameters of LVH were detected in group 2 (all p ˂ 0.05).Conclusion. The direction of the associations between circulating adipokines and LVH varies with the preclinical stage of HF. The data obtained may reflect a relationship between heart remodeling in response to molecular mechanisms of inflammation and IR in obese patients at the certain stage of cardiovascular continuum.
Prevalence of hearing loss in schoolchildren: populational study and global estimates
Authors: Chibisova S. S. , Alsharjabi E. , Zyuzin A. S. , Markova T. G. , Popadyuk V. I. , Tavartkiladze G. A. 
Published: October 2022

Introduction. Hearing impairments in schoolchildren affects learning and communication. Reliable data on hearing loss prevalence in this group are necessary for planning the audiological care.Aim. To compare data from a population-based study of the hearing loss prevalence in schoolchildren and estimates from the Global Burden of Disease (GBD) study in the Republic of Yemen.Materials and methods. Data extraction was performed from the GBD database on the prevalence of bilateral hearing loss >20 dB among children aged 5–9 years in the Republic of Yemen. A two-stage study of hearing loss prevalence among primary school students aged 6–9 years in Sana’a, Republic of Yemen, in a sample of 2200 children using screening audiometry at 20 dB at 0.5, 1, 2, and 4 kHz is school settings, followed by tonal threshold audiometry and tympanometry in a specialized clinic. Hearing loss was assessed as unilateral or bilateral when hearing thresholds were higher than 25 dB.Results. According to GBD estimates, bilateral hearing impairment in this population is 2.2%, including 1.4% of mild degree. According to the results of a Yemenian population study, mild and moderate hearing impairment was detected in 10.6%, of which 6.8% – unilateral, 3.8% – bilateral hearing loss. There were no cases of severe or profound hearing loss. The prevalence of otitis media with effusion was 6%, chronic suppurative otitis media – 1.8%, sensorineural hearing loss – 1.6%.Conclusion. Bilateral hearing impairments occur in 2.2–3.8% of primary school students, with unilateral impairments the prevalence is up to 10.6%. Of these, at least 65% have conductive hearing loss due to pathology of the middle ear. The data obtained will improve the efficiency of ear and hearing care for schoolchildren.