bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromophoric Dissolved Organic Matter and Dissolved Organic Carbon in Lakes Across an Elevational Gradient From the Mountains to the Sea
Authors: Juetten Kyle , Strecker Angela L. , Harrison Aaron , Landram Zachary , De Bruyn Warren J. , Clark Catherine D. 
Published: December 2022

AbstractDissolved organic matter (DOM) in lakes across elevation gradients is a complex function of topography, climate, vegetation coverage, land use, and lake properties. To examine sources and processing of DOM from sea level to mountain lakes (3–1,574 m), we measured dissolved organic carbon (DOC) concentrations and chromophoric dissolved organic matter (CDOM) optical properties, lake characteristics, and water quality parameters in 62 freshwater lakes in the Pacific Northwest, USA. Higher elevation lakes had lower DOC concentrations and absorbance. These lakes had higher forest cover and minimal wetlands in their watershed, in addition to low nutrients, water temperatures, and chlorophyll a in the lake itself. Two humic‐like and one protein‐like fluorescent component were identified from excitation‐emission matrix spectroscopy. The index of recent autochthonous contribution (BIX), fluorescence index (FIX), and SR optical indices showed that most lakes were dominated by terrestrially derived material. The humification index (HIX) and specific ultra‐violet absorbance (SUVA254) were consistent with more aromatic humic CDOM at lower elevations. The lower fluorescence of humic‐like components at higher elevation was attributed to lower inputs from vegetation. The relative contribution of the protein‐like component increased at higher elevation. This may be due to reduced allochthonous terrestrial inputs relative to in situ production of autochthonous material or increased photochemical/biological degradation of allochthonous material. Differences in optical characteristics associated with the amount and source of CDOM were observed across the elevational gradient. These differences were driven by characteristics at both within‐lake and watershed scales.
An Updated CLUBB PDF Closure Scheme to Improve Low Cloud Simulation in CAM6
Authors: Li Te , Wang Minghuai , Guo Zhun , Yang Ben , Xu Yifei , Han Xiaomen , Sun Jianning 
Published: December 2022

AbstractCloud Layers Unified By Binormals (CLUBB) adopts the joint probability density function (PDF) to close higher‐order turbulence and diagnose the cloud fraction. CLUBB assumes the equal PDF width of vertical velocity () as the default closure scheme, called Analytic Double Gaussian 1 (ADG1). In this study, a new unequal width PDF closure scheme is adopted in CLUBB, in which the PDF width of is assumed to be associated with skewness and kurtosis of vertical velocity. Offline calculation shows that the updated closure scheme improves the tail in the joint PDF and produces the saturation area from the joint PDF of the total water and liquid water potential temperature closer to that derived from large‐eddy simulations than ADG1. The new PDF scheme implemented in Community Atmosphere Model Version 6 is shown to increase low cloud fraction by 20%–30% in most stratocumulus‐to‐cumulus transition regions. The updated closure scheme is found to improve the low cloud simulation by simulating more symmetric turbulence in stratocumulus and stronger turbulent transport in shallow cumulus. Furthermore, the updated closure scheme enhances the cloud fraction near the cloud base by interacting with the microphysical scheme. Our work indicates the importance of continuous improvement in the PDF closures for higher‐order turbulence schemes.
Oligocene/Early Miocene E/W‐Shortening in the Oman Mountains Related to Oblique Arabia‐India Convergence
Authors: Scharf Andreas , Mattern Frank , Bolhar Robert , Callegari Ivan , Mattern Paul , Ring Uwe 
Published: December 2022

AbstractField survey, literature review, geological map interpretation, GPlates reconstruction and LA‐ICP‐MS U‐Pb dating of synkinematic calcite demonstrate that ∼E/W‐shortening in eastern Oman was significant and related to oblique convergence of Arabia and India from 32.5 to 20 Ma. Approximately N/S‐striking contractional structures, WNW to NNW‐striking sinistral faults and ∼E/W‐striking normal faults characterize a ∼250 km × ∼50 km wrench corridor in the eastern Oman Mountains (Hajar Wrench Corridor, HWC). Numerous faults/folds indicate that deformation of the HWC is widely distributed but concentrated along WNW to NNW‐striking major faults at the SW margin of the Saih Hatat Dome, forming the Hajar Shear Zone, which reactivated basement faults. GPlates reconstructions reveal that N‐drifting India rotated 8° counter‐clockwise with respect to fixed Arabia from 32.5 to 20 Ma, leading to a minimum of 100 km E/W convergence between both plates. This convergence created the sinistral HWC with a displacement of a few to several tens of kilometers. Independently from the GPlates time constraints, two U‐Pb ages of synkinematic calcites, crystallized along faults during HWC movement, yield compatible ages of 30.08 ± 0.47 and 22.31 ± 2.15 Ma (2 standard error). E/W‐shortening also affected the northern Oman Mountains, creating the ∼N/S‐striking Hagab Thrust in the Musandam Peninsula and the Jabal Hafit Anticline.
Late Miocene Exhumation of the Western Cordillera, Ecuador, Driven by Increased Coupling Between the Subducting Carnegie Ridge and the South American Continent
Authors: Margirier Audrey , Strecker Manfred R. , Reiners Peter W. , Thomson Stuart N. , Casado Ismael , George Sarah W. M. , Alvarado Alexandra 
Published: December 2022

AbstractThe subduction of bathymetric highs, such as aseismic ridges, leads to far‐reaching changes in the dynamics of subduction zones with increased plate coupling and deformation in the upper plate. Subduction of the submarine Carnegie Ridge on the Nazca Plate has fundamentally impacted late Cenozoic magmatism and tectonic activity in the northern Andes. However, the timing of onset of Carnegie Ridge subduction has been a matter of debate. Time‐temperature inverse modeling of new thermochronological data from the Western Cordillera of Ecuador reveals two phases of cooling separated by isothermal conditions. The first cooling phase postdates early and middle Miocene magmatism in the Western Cordillera and is attributed to post‐magmatic thermal relaxation. The second cooling phase started after 6 Ma. Inferred to record the onset of tectonically controlled rock uplift and exhumation in the Western Cordillera, this phase is coeval with the last cooling phase recorded in the Eastern Cordillera. Based on these findings, we suggest that the onset of subduction of the Carnegie Ridge at ∼6–5 Ma increased plate coupling at the subduction interface, promoting shortening, regional rock uplift, and exhumation in the northern Andes. Overall, our results highlight the essential role of bathymetric highs in driving regional upper‐plate deformation at non‐collisional convergent plate margins.
The skin is no barrier to mixtures: Air pollutant mixtures and reported psoriasis or eczema in the Personalized Environment and Genes Study (PEGS)
Authors: Lowe Melissa E. , Akhtari Farida S. , Potter Taylor A. , Fargo David C. , Schmitt Charles P. , Schurman Shepherd H. , Eccles Kristin M. , Motsinger-Reif Alison , Hall Janet E. , Messier Kyle P. 
Published: December 2022

AbstractBackgroundAutoimmune (AI) diseases appear to be a product of genetic predisposition and environmental triggers. Disruption of the skin barrier causes exacerbation of psoriasis/eczema. Oxidative stress is a mechanistic pathway for pathogenesis of the disease and is also a primary mechanism for the detrimental effects of air pollution.MethodsWe evaluated the association between autoimmune skin diseases (psoriasis or eczema) and air pollutant mixtures in 9060 subjects from the Personalized Environment and Genes Study (PEGS) cohort. Pollutant exposure data on six criteria air pollutants are publicly available from the Center for Air, Climate, and Energy Solutions and the Atmospheric Composition Analysis Group. For increased spatial resolution, we included spatially cumulative exposure to volatile organic compounds from sites in the United States Environmental Protection Agency Toxic Release Inventory and the density of major roads within a 5 km radius of a participant’s address from the United States Geological Survey. We applied logistic regression with quantile g-computation, adjusting for age, sex, diagnosis with an autoimmune disease in family or self, and smoking history to evaluate the relationship between self-reported diagnosis of an AI skin condition and air pollution mixtures.ResultsOnly one air pollution variable, sulfate, was significant individually (OR = 1.06,p = 3.99E−2); however, the conditional odds ratio for the combined mixture components of PM2.5(black carbon, sulfate, sea salt, and soil), CO, SO2, benzene, toluene, and ethylbenzene is 1.10 (p-value = 5.4E−3).SignificanceWhile the etiology of autoimmune skin disorders is not clear, this study provides evidence that air pollutants are associated with an increased prevalence of these disorders. The results provide further evidence of potential health impacts of air pollution exposures on life-altering diseases.Significance and impact statementThe impact of air pollution on non-pulmonary and cardiovascular diseases is understudied and under-reported. We find that air pollution significantly increased the odds of psoriasis or eczema in our cohort and the magnitude is comparable to the risk associated with smoking exposure. Autoimmune diseases like psoriasis and eczema are likely impacted by air pollution, particularly complex mixtures and our study underscores the importance of quantifying air pollution-associated risks in autoimmune disease.
A failure of sleep-dependent consolidation of visuoperceptual procedural learning in young adults with ADHD
Authors: Ballan Ranin , Durrant Simon J. , Stickgold Robert , Morgan Alexandra , Manoach Dara S. , Gabay Yafit 
Published: December 2022

AbstractADHD has been associated with cortico-striatal dysfunction that may lead to procedural memory abnormalities. Sleep plays a critical role in consolidating procedural memories, and sleep problems are an integral part of the psychopathology of ADHD. This raises the possibility that altered sleep processes characterizing those with ADHD could contribute to their skill-learning impairments. On this basis, the present study tested the hypothesis that young adults with ADHD have altered sleep-dependent procedural memory consolidation. Participants with ADHD and neurotypicals were trained on a visual discrimination task that has been shown to benefit from sleep. Half of the participants were tested after a 12-h break that included nocturnal sleep (sleep condition), whereas the other half were tested after a 12-h daytime break that did not include sleep (wakefulness condition) to assess the specific contribution of sleep to improvement in task performance. Despite having a similar degree of initial learning, participants with ADHD did not improve in the visual discrimination task following a sleep interval compared to neurotypicals, while they were on par with neurotypicals during the wakefulness condition. These findings represent the first demonstration of a failure in sleep-dependent consolidation of procedural learning in young adults with ADHD. Such a failure is likely to disrupt automatic control routines that are normally provided by the non-declarative memory system, thereby increasing the load on attentional resources of individuals with ADHD.
TSSK4 upregulation in alveolar epithelial type-II cells facilitates pulmonary fibrosis through HSP90-AKT signaling restriction and AT-II apoptosis
Authors: Chen Huifang , He Andong , Li Haoyang , Chen Honglv , Xie Huancheng , Luo Liping , Huang Yuyi , Chen Jiaqian , Guan Jieying , He Qiaoling , Ma Jianjuan , Ou Changxing , Tao Ailin , Yan Jie 
Published: December 2022

AbstractAlveolar epithelial injury is one of the important pathological changes in idiopathic pulmonary interstitial fibrosis (IPF), but the regulatory mechanism remains unclear. Here, we reported that alveolar epithelial type-II cells (AT II) play important roles in pathological process of pulmonary fibrosis. Through iTRAQ (isobaric tagging for relative and absolute quantification) quantitative proteomics, TSSK4 was identified to be upregulated in bleomycin-induced fibrotic mice model, which was further confirmed in clinical IPF patients’ tissue specimens. TSSK4 is a germ-related protein, but its expression in other tissues and the association with other diseases are not reported. Immunofluorescence staining showed that TSSK4 selectively expressed in AT-II cells, which are essential for inflammation-induced AT-II loss during fibrosis. Luciferase assay and other molecular biological experiments proved that TSSK4 expression is regulated by TNF-α-mediated NF-κB signaling. The TSSK4 kinase activity is found to be closely related to the function of HSP90-AKT pathway that TSSK4 can phosphorylate its substrate HSP90β on serine 255, to inhibit the ATPase activity of HSP90β and reduce its molecular chaperone function on AKT. Under this condition, kinase activity of AKT is diminished to interfere its survival function, subsequently facilitating AT-II cellular apoptosis through the mitochondrial death machinery. Our findings highlight the importance of TSSK4 in regulating pulmonary fibrosis by facilitating AT-II loss through HSP90-AKT signaling, all of which suggest TSSK4 and the regulating mechanism as attractive targets for the clinical intervention of pulmonary injury and fibrosis.
NRG1 knockdown rescues PV interneuron GABAergic maturation deficits and schizophrenia behaviors in fetal growth restriction mice
Authors: Dong Jianfeng , Chen Wen , Liu Nana , Chang Shujuan , Zhu Wei , Kang Jiuhong 
Published: December 2022

AbstractSchizophrenia is a highly debilitating mental disorder, those who experienced fetal growth restriction (FGR) in the early stage of life have a greater probability of schizophrenia. In this study, FGR mice showed hyperactivity in locomotor activity test, sociability dysfunction in three chamber test and nesting social behavior tests, cognition decline in Morris water maze and impaired sensory motor gating function in prepulse inhibition test. Mechanistic studies indicated that the number of parvalbumin (PV) interneuron was significantly reduced in FGR mouse media prefrontal cortex (mPFC). And the mRNA and protein level of neuregulin 1(NRG1), which is a critical schizophrenia gene, increased significantly in FGR mouse mPFC. Furthermore, NRG1 knockdown in FGR mouse mPFC improved PV interneuron GABAergic maturation and rescued schizophrenia behaviors including hyperactivity, social novelty defects, cognition decline, and sensorimotor gating deficits in FGR mice. This study indicates that mPFC NRG1 upregulation is one of the main causes of FGR-induced schizophrenia, which leads to significant reduction of PV interneuron number in mPFC. NRG1 knockdown in mPFC significantly rescues schizophrenia behaviors in FGR mouse. This study thus provides a potential effective therapy target or strategy for schizophrenia patients induced by FGR.
Primary cilia suppress Ripk3-mediated necroptosis
Authors: Kieckhöfer Emilia , Slaats Gisela G. , Ebert Lena K. , Albert Marie-Christine , Dafinger Claudia , Kashkar Hamid , Benzing Thomas , Schermer Bernhard 
Published: December 2022

AbstractCilia are sensory organelles that project from the surface of almost all cells. Nephronophthisis (NPH) and NPH-related ciliopathies are degenerative genetic diseases caused by mutation of cilia-associated genes. These kidney disorders are characterized by progressive loss of functional tubular epithelial cells which is associated with inflammation, progressive fibrosis, and cyst formation, ultimately leading to end-stage renal disease. However, disease mechanisms remain poorly understood. Here, we show that targeted deletion of cilia in renal epithelial cells enhanced susceptibility to necroptotic cell death under inflammatory conditions. Treatment of non-ciliated cells with tumor necrosis factor (TNF) α and the SMAC mimetic birinapant resulted in Ripk1-dependent cell death, while viability of ciliated cells was almost not affected. Cell death could be enhanced and shifted toward necroptosis by the caspase inhibitor emricasan, which could be blocked by inhibitors of Ripk1 and Ripk3. Moreover, combined treatment of ciliated and non-ciliated cells with TNFα and cycloheximide induced a cell death response that could be partially rescued with emricasan in ciliated cells. In contrast, non-ciliated cells responded with pronounced cell death that was blocked by necroptosis inhibitors. Consistently, combined treatment with interferon-γ and emricasan induced cell death only in non-ciliated cells. Mechanistically, enhanced necroptosis induced by loss of cilia could be explained by induction of Ripk3 and increased abundance of autophagy components, including p62 and LC3 associated with the Ripk1/Ripk3 necrosome. Genetic ablation of cilia in renal tubular epithelial cells in mice resulted in TUNEL positivity and increased expression of Ripk3 in kidney tissue. Moreover, loss of Nphp1, the most frequent cause of NPH, further increased susceptibility to necroptosis in non-ciliated epithelial cells, suggesting that necroptosis might contribute to the pathogenesis of the disease. Together, these data provide a link between cilia-related signaling and cell death responses and shed new light on the disease pathogenesis of NPH-related ciliopathies.
Dual-responsive disassembly of core-shell nanoparticles with self-supplied H2O2 and autocatalytic Fenton reaction for enhanced chemodynamic therapy
Authors: Peng Mengran , Ju Enguo , Xu Yanteng , Wang Yuqin , Lv Shixian , Shao Dan , Wang Haixia , Tao Yu , Zheng Yue , Li Mingqiang 
Published: December 2022

AbstractChemodynamic therapy holds great potential for cancer treatment due to its reliable curative effects, minimal invasiveness, and few systemic side effects. However, the limited amount of intracellular H2O2 makes achieving high-performance chemodynamic therapy challenging. Herein, we report a core-shell nanoplatform with dual-responsive disassembly that self-supplies H2O2 and undergoes an autocatalytic Fenton reaction for enhanced chemodynamic therapy. The platform was designed by coating glucose oxidase-mimicking nanozyme gold nanoparticles (AuNPs) with a metal-polyphenol network (Au@MPN). Both ATP and low pH can disassemble the Au@MPN to release Fe(III), which can then be reduced into Fe(II) by the simultaneously released tannic acid (TA). In particular, the exposed AuNPs can catalyze the oxidation of intracellular glucose to produce H2O2. Subsequently, Fe(II) and the self-supplied H2O2 induce an efficient Fenton reaction for chemodynamic therapy by generating hydroxyl radicals (•OH) that are highly toxic to cancer cells. Moreover, tumor growth can be effectively suppressed after both intratumoral and intravenous Au@MPN administration. Additionally, metastatic melanoma lung tumors could be inhibited by intratracheal instillation of Au@MPN. Thus, this work not only reports a facile method to construct a chemodynamic agent with self-supplied H2O2 and high therapeutic efficiency but also provides insight into the design of nanoplatforms with enhanced efficiency for chemodynamic therapy.
Specific gut microbiome signatures and the associated pro-inflamatory functions are linked to pediatric allergy and acquisition of immune tolerance
Authors: De Filippis Francesca , Paparo Lorella , Nocerino Rita , Della Gatta Giusy , Carucci Laura , Russo Roberto , Pasolli Edoardo , Ercolini Danilo , Berni Canani Roberto 
Published: December 2022

AbstractUnderstanding the functional potential of the gut microbiome is of primary importance for the design of innovative strategies for allergy treatment and prevention. Here we report the gut microbiome features of 90 children affected by food (FA) or respiratory (RA) allergies and 30 age-matched, healthy controls (CT). We identify specific microbial signatures in the gut microbiome of allergic children, such as higher abundance of Ruminococcus gnavus and Faecalibacterium prausnitzii, and a depletion of Bifidobacterium longum, Bacteroides dorei, B. vulgatus and fiber-degrading taxa. The metagenome of allergic children shows a pro-inflammatory potential, with an enrichment of genes involved in the production of bacterial lipo-polysaccharides and urease. We demonstrate that specific gut microbiome signatures at baseline can be predictable of immune tolerance acquisition. Finally, a strain-level selection occurring in the gut microbiome of allergic subjects is identified. R. gnavus strains enriched in FA and RA showed lower ability to degrade fiber, and genes involved in the production of a pro-inflammatory polysaccharide. We demonstrate that a gut microbiome dysbiosis occurs in allergic children, with R. gnavus emerging as a main player in pediatric allergy. These findings may open new strategies in the development of innovative preventive and therapeutic approaches. Trial: NCT04750980.
Overcoming C60-induced interfacial recombination in inverted perovskite solar cells by electron-transporting carborane
Authors: Ye Fangyuan , Zhang Shuo , Warby Jonathan , Wu Jiawei , Gutierrez-Partida Emilio , Lang Felix , Shah Sahil , Saglamkaya Elifnaz , Sun Bowen , Zu Fengshuo , Shoaee Safa , Wang Haifeng , Stiller Burkhard , Neher Dieter , Zhu Wei-Hong , Stolterfoht Martin , Wu Yongzhen 
Published: December 2022

AbstractInverted perovskite solar cells still suffer from significant non-radiative recombination losses at the perovskite surface and across the perovskite/C60 interface, limiting the future development of perovskite-based single- and multi-junction photovoltaics. Therefore, more effective inter- or transport layers are urgently required. To tackle these recombination losses, we introduce ortho-carborane as an interlayer material that has a spherical molecular structure and a three-dimensional aromaticity. Based on a variety of experimental techniques, we show that ortho-carborane decorated with phenylamino groups effectively passivates the perovskite surface and essentially eliminates the non-radiative recombination loss across the perovskite/C60 interface with high thermal stability. We further demonstrate the potential of carborane as an electron transport material, facilitating electron extraction while blocking holes from the interface. The resulting inverted perovskite solar cells deliver a power conversion efficiency of over 23% with a low non-radiative voltage loss of 110 mV, and retain >97% of the initial efficiency after 400 h of maximum power point tracking. Overall, the designed carborane based interlayer simultaneously enables passivation, electron-transport and hole-blocking and paves the way toward more efficient and stable perovskite solar cells.
Near-critical spreading of droplets
Authors: Saiseau Raphael , Pedersen Christian , Benjana Anwar , Carlson Andreas , Delabre Ulysse , Salez Thomas , Delville Jean-Pierre 
Published: December 2022

AbstractWe study the spreading of droplets in a near-critical phase-separated liquid mixture, using a combination of experiments, lubrication theory and finite-element numerical simulations. The classical Tanner’s law describing the spreading of viscous droplets is robustly verified when the critical temperature is neared. Furthermore, the microscopic cut-off length scale emerging in this law is obtained as a single free parameter for each given temperature. In total-wetting conditions, this length is interpreted as the thickness of the thin precursor film present ahead of the apparent contact line. The collapse of the different evolutions onto a single Tanner-like master curve demonstrates the universality of viscous spreading before entering in the fluctuation-dominated regime. Finally, our results reveal a counter-intuitive and sharp thinning of the precursor film when approaching the critical temperature, which is attributed to the vanishing spreading parameter at the critical point.