bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tramadol rescues visual cortex gliosis and modulates ocular lipido-inflammatory responses in a rat model of paradoxical sleep deprivation
Authors: Edem Edem Ekpenyong , James Emem Grace , Anyanwu Collins-Kevin Chukwudi , Adedokun Mujeeb Adekunle , Fafure Adedamola Adediran , Akinluyi Elizabeth Toyin 
Published: September 2022

Abstract

Individually, sleep deprivation and sub-chronic tramadol exposure have been reported to impair visual processes, however the underlying mechanisms of their combined effects are largely unknown. Thus, this study investigated the role of tramadol hydrochloride on lipid-immune activities in the ocular tissue and visual cortex of sleep-deprived periadolescent rats. Sixty female periadolescent Wistar rats were either sleep-deprived with or without tramadol treatment. Following euthanasia, brain and whole eye tissues were collected for biochemical and immunohistochemical assays. Results revealed impaired ocular tissue lipid profile following sleep deprivation (SD). Sleep deprivation also induced lipid peroxidation; upregulated apolipoprotein E (ApoE), and nuclear factor kappa B (NF-κB) 1 levels in the ocular tissue. Furthermore, chronic SD exposure triggered gliosis with marked increase in astrocyte and microglia counts in the visual cortex. However, treatment with tramadol restored ocular tissue lipid function markers, downregulated ocular tissue NF-κB levels, as well as ameliorated sleep deprivation-induced gliosis in the visual cortex. Taken together, this study demonstrates the role of tramadol in improving inflammatory processes and lipid homeostasis in the visual system by modulating ocular tissue ApoE and NF-κB signalling, and attenuating gliosis in the visual cortex of sleep-deprived rats.
An intelligent hybridized computing techniques for the prediction of roadway traffic noise based on non-linear mutual information
Authors: Umar Ibrahim Khalil , Nourani Vahid , Gökçekuş Hüseyin , ABBA SANI 
Published: September 2022

Abstract
A reliable traffic noise prediction model is one of the decision-making tools used in providing a noise friendly environment. In this study, four linear-nonlinear hybrid models were proposed to capture both linear and nonlinear patterns of the data by summing up the predicted traffic noise from the multilinear regression (MLR) and estimated residuals from four artificial intelligence (AI)-based models. The input variables for the models were volumes of cars, medium vehicles, buses, heavy vehicles, and average speed. Prior to the development of the hybrid model, the potential of Boosted Regression Tree (BRT), Feed Forward Neural Network (FFNN), Gaussian Process Regression (GPR), Support Vector Regression (SVR) and Linear regression models for traffic noise prediction were evaluated and compared with each other. The performances of the single and hybrid models were evaluated using the Nash-Sutcliffe efficiency (NSE), root mean square error (RMSE), mean absolute error (MAE) and relative root mean square error (rRMSE). The results showed that, the hybrid models provide better prediction capability than both the linear and nonlinear models in both calibration and verification stages. MLR-GPR hybrid demonstrated better prediction skill than all other hybrid models with NSE, RMSE, MAE and rRMSE values of 0.9312, 0.0427, 0.0347 and 7.4%, respectively. The study found that, the efficiency of the linear models could be improved up to 27.26% when they are hybridized with the nonlinear models.
An Unusual Effect of Hofmeister Series Salts on the Stability of
Toxoplasma gondii Ferredoxin NADP+ Reductase
Authors: Singh Kulwant , Akhtar Md. Sohail 
Published: September 2022

Background:
The ionic interactions play an important role in the stabilization of the native
conformation of proteins. Toxoplasma gondii Ferredoxin NADP+ Reductase (TgFNR) remains stable
at pH 4.0. However, such modulation of ionic interactions leads to compaction and non-cooperativity
in its folding.

Objective:
To gain insights into the role of ionic interactions in the modulation of structure and
thermodynamic stability of TgFNR.

Methods:
Protein preparations, circular dichroism and fluorescence spectroscopy were used to
determine salt-induced changes in the structure and stability of TgFNR.

Results:
The kosmotropic salts (sodium fluoride and sodium sulphate) appear to induce the biphasic
response on the structure and stability of TgFNR. At pH about 4.0, the addition of low concentrations
of kosmotropic salts significantly perturbs the existing native-like secondary structure of TgFNR,
whereas higher quantities of salt reversed the denaturing impact. This is a one-of-a-kind situation we
are unaware of in any other protein. The urea-induced unfolding of TgFNR in the presence of a low
dose of salt (100 mM) drastically affected the protein's thermodynamic stability at neutral pH. The
increased salt concentrations, on the other hand, reversed the destabilizing effect.

Conclusion:
Our findings imply that electrostatic interactions are exceptionally significant for the
TgFNR stability, however, render highly unusual behavior of Hofmeister series salts, indicating a
possible crucial role of salt bridges in the stabilization of different conformations of the protein.
A Novel Tumor Mutation Burden Related lncRNA Signature Identified Prognosis and Tumor Immune Microenvironment Features in Clear Cell Renal Cell Carcinoma
Authors: Lin Lin , Wu Xiao-Hui , Zhu Jun-Ming , Chen Shao-Hao , Chen Ye-Hui , Lin Fei , Xue Xue-Yi , Wei Yong , Xu Ning , Zheng Qing-Shui , Sun Xiong-Lin 
Published: September 2022

Background:Emerging evidence indicates that long noncoding RNA (lncRNA) plays an important biological role in clear cell renal cell carcinoma (ccRCC); however, the clinical value of tumor mutation burden-related lncRNA in ccRCC patients is unknown yet.Method:Somatic mutation profiles and lncRNA expression data of ccRCC were downloaded from the TCGA database. We retrospectively analyzed lncRNA expression data and survival information from 116 patients with ccRCC fromJanuary 2013 to January 2014. Univariate and multivariate Cox regression analyses were performed to construct lncRNA signature, and the prognosis value was determined by Kaplan-Mayer and receiver operating characteristic curve (ROC) analysis.Results:Based on 160 differentially expressed TMB-related lncRNAs, two TMB-related molecular clusters with distinct immune checkpoints expression and immune cells infiltration were established for ccRCC patients. Moreover, a novel TMB-related lncRNA signature was constructed based on five lncRNAs for individualized prognosis assessment. High-risk group represents significantly worse overall survival in all cohorts. The area under the ROC curve was 0.716, 0.775 and 0.744 in the training cohort, testing cohort and TCGA cohort, respectively. Results of qRT-PCR successfully validated the expression levels of AP002360.3, LINC00460, AL590094.1, LINC00944 and LINC01843 in HK-2, 786-O, 769-P and ACHN cells. More importantly, the predictive performance of TMB-related lncRNA signature was successfully validated in an independent cohort of 116 ccRCC patients at our institution.Conclusion:This study successfully developed and validated a novel TMB-related lncRNA signature for individualized prognosis assessment of ccRCC patients.
Tissue Factor and COVID-19: An Update
Authors: Mackman Nigel , Sachetto Ana Teresa Azevedo 
Published: September 2022

Abstract:
The coronavirus 2019 (COVID-19) pandemic is caused by severe acute respiratory syn-drome coronavirus 2 (SARS-CoV-2) virus. Infection with SARS-CoV-2 is associated with acute respiratory distress syndrome, thrombosis and a high rate of mortality. Thrombotic events increase with severity. Tissue factor (TF) expression is increased during viral and bacterial infections. This review summarizes studies that have examined TF expression in response to SARS-CoV-2 infec-tion. SARS-CoV-2 virus and its proteins upregulate TF mRNA, protein and activity in a variety of cells, including bronchial epithelial cells, neutrophils, monocytes, macrophages, endothelial cells and adventitial fibroblasts. COVID-19 patients have increased TF expression in lungs, bron-choalveolar lavage fluid and circulating extracellular vesicles. The increase in TF was associated with coagulation activation markers, thrombosis, inflammatory markers, severity of disease and mortality. Taken together, the studies suggest that TF plays a central role in thrombosis in COVID-19. TF may be a useful prognostic marker and therapeutic target to reduce thrombosis and inflam-mation.
Direct Vinylation of Activated Methines by a Vinylsulfonium Salt
Authors: Xie Chunsong , Hu Jiahong , Zhu Xiaole , Wu Song , Xu Yujia , Ye Hongyi 
Published: September 2022

Abstract:
Vinylation of activated methines is a synthetically useful and challenging transformation.
Traditional methods for this transformation always encounter multiple steps, limited generality and/or
handling gaseous substrates. Herein, we report by using diphenyl(vinyl)sulfonium triflate as a vinylation
component, a one-step direct vinylation of activated methines can be realized under mild conditions.
This reaction, compatible with several kinds of activated methines, can lead to the construction
of vinylated quaternary carbon centers concisely.
Molecular Hydrogen Promotes Adipose-derived Stem Cell Myogenic
Differentiation via Regulation of Mitochondria
Authors: Yang Yu-Xia , Fei Wen-Yong , Liu Ming-Sheng , Zhang Yu-Cheng , Gao Rang-Shan , Hu Yang-Yang , Pang Er-Kai , Hou Lei 
Published: September 2022

Background:
Acute skeletal muscle injuries are common physical or sports traumas. Cellular
therapy has excellent potential for regeneration after skeletal muscle injury. Adipose-derived stem cells
(ADSCs) are a more accessible type of stem cell. However, it has a low survival rate and differentiation
efficiency in the oxidative stress-rich microenvironment after transplantation. Although molecular hydrogen
(H2) possesses anti-inflammatory and antioxidant biological properties, its utility in mitochondrial
and stem cell research has not been adequately explored.

Objective:
This study aimed to reveal the role of H2 on adipose-derived stem cells' myogenic differentiation.

Methods:
The protective effects of H2 in ADSCs were evaluated by MTT assay, live-dead cell staining,
western blot analysis, immunofluorescence staining, confocal imaging, and transmission electron microscopy.

Results:
An appropriate volume fraction of H2 significantly decreased mitochondrial reactive oxygen
species (ROS) levels, increased the number of mitochondria, and promoted mitophagy, thus enhancing
the survival and myogenic differentiation of ADSCs.

Conclusion:
This study reveals the application potential of H2 in skeletal muscle diseases or other pathologies
related to mitochondrial dysfunction.
The Association Between Qtc, Qtd, TPE, And Fragmented QRS Before
And After PPCI With Hospital Mortality In STEMI Patients
Authors: Khaheshi Isa , Namazi Mohammad Hassan , Salehi Ayoub , Akbarzadeh Mohammad Ali , Parsa Saeed Alipour , Safi Morteza , Vakili Hossein , Saadat Habibollah , Eslami Vahid , Kiaee Farshid Heidarpour , Nourian Saeed , Sohrabifar Nasim 
Published: September 2022

Introduction:
ST-elevation myocardial infarction (STEMI) is known to be associated
with significant arrhythmia and consequent mortality. QT prolongation is a risk factor for arrhythmia
in STEMI patients who underwent primary percutaneous coronary intervention (PPCI). The aim
of this investigation was to evaluate the association of corrected QT interval (QTc), QT dispersion
(QTd), T-wave peak to end (TPE), and fragmented QRS with mortality in these patients.

Methods:
Eligible patients with the characteristic symptoms of STEMI who underwent PPCI were
included. QTc, QTd, TPE, and fragmented QRS were measured before and after the PPCI. These
predictors were compared between patients who died during hospitalization and discharged patients.

Results:
After coronary angiography, 10 patients (4%) died during the hospitalization after PPCI.
Comparing the non-survivers and discharged patients in terms of arrhythmia predictors showed that
the mean QT dispersion and TPE before intervention were significantly higher in the non-survivors.
Also, the number of patients who experienced fragmented QRS before and after the intervention
was significantly higher in the non-survivors.

Conclusion:
These data suggested that evaluating such arrhythmia predictors, especially before
PPCI, could be used as a predictor of mortality in STEMI patients who underwent PPCI.
Effect of Silver Nanoparticles Synthesized by ‘Green’ Methods on the Growth of in vitro Culture of Betula pendula L. whole Plants
Authors: Przhevalskaya Darya A. , Bandarenka Uladzislau Y. , Shashko Antonina Y. , Charnysh Maryia A. , Smolich Ihar I. , Sokolik Anatoliy I. , Konstantinov Andrei V. , Padutov Vladimir E. , Demidchik Vadim V. 
Published: September 2022

Background:
Metal nanoparticles, such as silver nanoparticles obtained by “green” nanosynthesis, have been increasingly used in research and practice in recent years due to their high biocompatibility and low toxicity. It is important to understand how green nanoparticles have regulatory effects on all groups of living systems, including plants. One of the key questions is how silver nanoparticles obtained by green methods modify plant growth in various cultivation and biotechnological systems, such as in vitro culture.

Objective:
The aim of this study was to establish how in vitro culture of birch plants (Betula pendula Roth) reacts to different levels of silver nanoparticles synthesized by green methods (based on plant extracts) and chemical approaches.

Methods:
The paper examined the nodal segments of silver birch Betula pendula Roth grown on Woody Plant Medium (WPM) with the addition of silver nanoparticles (0.3-300 mg L-1). After 30 days of cultivation in an in vitro environment, the growth of shoots and roots was measured. Silver nanoparticles were synthesized using L-ascorbic acid (reducing agent) and polyvinylpyrrolidone (PVP; stabilizer), as well as with needle extract (as a reducing agent and stabilizer).

Results:
Chemical nanosynthesis based on PVP and L-ascorbate, as well as green nanosynthesis using extract of spruce needles made it possible to obtain spherical nanoparticles with similar physical parameters. Low levels of AgNPs (0.3-10 mg L-1) synthesized by chemical techniques (PVP and L-ascorbate) stimulated the growth of birch shoots. In this case, the maximum stimulating effect on shoot growth was found at 10 mg of L-1 AgNPs (250-300% stimulation compared to the control). Under higher levels of nanoparticles (30-300 mg L-1), the stimulating effect decreased. Concentrations over 300 mg of L-1 inhibited the growth of birch plants. Very similar effects were observed in roots.
In experiments with nanoparticles synthesized using spruce needle extract, it was shown that low concentrations of AgNPs (0.3 and 1 mg L-1) did not cause a significant change in the size of birch shoots and roots. At the same time, higher levels of silver nanoparticles (3-300 mg L-1) significantly stimulated growth.

Conclusion:
The present study demonstrates the production of stable silver nanoparticles based on PVP and L-ascorbic acid, as well as an extract of Betula pendula needles. The resulting nanoparticles have a uniform shape and distribution. The presence of AgNP (1-300 mg L-1) in the nutrient media has a stimulating effect on Betula pendula shoot and root growth.
Psychological Disorders of Patients With Allergic Rhinitis in Chengdu, China: Exploratory Research
Authors: Huang Heyin , Wang Yichen , Zhang Lanzhi , Zhang Qinxiu , Wu Xiaojuan , He Hengsheng 
Published: September 2022

Background
The number of patients with allergic rhinitis (AR) has exceeded 500 million worldwide due to the unstable curative effect that can easily produce mental and psychological disorders. However, most of the relevant existing literature is one-on-one retrospective analyses or targeted meta-analyses of AR with psychological disorders like irritability, depression, and anxiety, while “multi-hospital + interdisciplinary” multiple regression analyses are scarce.

Objective
This study aims to precisely identify the psychological disorders of patients with AR who were diagnosed and treated in the five most renowned hospitals in Chengdu, China over the past 5 years using 10 classification methods so as to attract attention and care from otolaryngologists.

Methods
The Symptom Checklist 90 (SCL-90) was used to group and score the mental state of 827 strictly screened patients with AR according to 9 classification criteria. The scores were then compared within groups. Intergroup comparisons were made between the study group and the Chinese norm, and the positive factors for psychological disorders were extracted. Four symptoms in the study group, that is, nasal itching, sneezing, clear discharge, and nasal congestion, were scored on a visual analog scale. Partial correlation analysis was performed between the extracted positive factors for psychological disorders and the symptom scores by the multiple regression statistical method.

Results
Among 827 patients, 124 (15%) had no mental health impairments, 176 (21.3%) had mild impairments, 474 (57.3%) had mild to moderate impairments, 41 (5%) had moderate to severe impairments, and 12 (1.4%) had severe impairments. The average score of the SCL-90 for all 827 patients was 2.64 (SD 0.25), which corresponded to mild to moderate mental health impairments. The 827 patients scored significantly higher for the 4 positive factors: depression, anxiety, psychosis, and other (sleep, diet). Depression was positively correlated with sneezing and clear discharge, anxiety was positively correlated with nasal itching and congestion, psychosis was positively correlated with nasal itching and sneezing, and other (sleep, diet) was positively correlated with clear discharge and nasal congestion.

Conclusions
Patients with AR have mild to moderate mental health impairments, with women and those with abnormal BMI, aged ≥45 years, with a monthly salary <¥5110 (US $700), with a disease duration <13 years, residing in urban areas, with a high school or above education, or who are indoor laborers being at high risk and requiring more care, follow-up, and comprehensive therapy from otolaryngologists.
The Value of Electronic Health Records Since the Health Information Technology for Economic and Clinical Health Act: Systematic Review
Authors: Modi Shikha , Feldman Sue S 
Published: September 2022

Background
Electronic health records (EHRs) are the electronic records of patient health information created during ≥1 encounter in any health care setting. The Health Information Technology Act of 2009 has been a major driver of the adoption and implementation of EHRs in the United States. Given that the adoption of EHRs is a complex and expensive investment, a return on this investment is expected.

Objective
This literature review aims to focus on how the value of EHRs as an intervention is defined in relation to the elaboration of value into 2 different value outcome categories, financial and clinical outcomes, and to understand how EHRs contribute to these 2 value outcome categories.

Methods
This literature review was conducted using PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). The initial search of key terms, EHRs, values, financial outcomes, and clinical outcomes in 3 different databases yielded 971 articles, of which, after removing 410 (42.2%) duplicates, 561 (57.8%) were incorporated in the title and abstract screening. During the title and abstract screening phase, articles were excluded from further review phases if they met any of the following criteria: not relevant to the outcomes of interest, not relevant to EHRs, nonempirical, and non–peer reviewed. After the application of the exclusion criteria, 80 studies remained for a full-text review. After evaluating the full text of the residual 80 studies, 26 (33%) studies were excluded as they did not address the impact of EHR adoption on the outcomes of interest. Furthermore, 4 additional studies were discovered through manual reference searches and were added to the total, resulting in 58 studies for analysis. A qualitative analysis tool, ATLAS.ti. (version 8.2), was used to categorize and code the final 58 studies.

Results
The findings from the literature review indicated a combination of positive and negative impacts of EHRs on financial and clinical outcomes. Of the 58 studies surveyed for this review of the literature, 5 (9%) reported on the intersection of financial and clinical outcomes. To investigate this intersection further, the category “Value–Intersection of Financial and Clinical Outcomes” was generated. Approximately 80% (4/5) of these studies specified a positive association between EHR adoption and financial and clinical outcomes.

Conclusions
This review of the literature reports on the individual and collective value of EHRs from a financial and clinical outcomes perspective. The collective perspective examined the intersection of financial and clinical outcomes, suggesting a reversal of the current understanding of how IT investments could generate improvements in productivity, and prompted a new question to be asked about whether an increase in productivity could potentially lead to more IT investments.
Virtual Care Initiatives for Older Adults in Australia: Scoping Review
Authors: Savira Feby , Gupta Adyya , Gilbert Cecily , Huggins Catherine E , Browning Colette , Chapman Wendy , Haines Terry , Peeters Anna 
Published: September 2022

Background
There has been a rapid shift toward the adoption of virtual health care services in Australia. It is unknown how widely virtual care has been implemented or evaluated for the care of older adults in Australia.

Objective
We aimed to review the literature evaluating virtual care initiatives for older adults across a wide range of health conditions and modalities and identify key challenges and opportunities for wider adoption at both patient and system levels in Australia.

Methods
A scoping review of the literature was conducted. We searched MEDLINE, Embase, PsycINFO, CINAHL, AgeLine, and gray literature (January 1, 2011, to March 8, 2021) to identify virtual care initiatives for older Australians (aged ≥65 years). The results were reported according to the World Health Organization’s digital health evaluation framework.

Results
Among the 6296 documents in the search results, we identified 94 that reported 80 unique virtual care initiatives. Most (69/80, 89%) were at the pilot stage and targeted community-dwelling older adults (64/79, 81%) with chronic diseases (52/80, 65%). The modes of delivery included videoconference, telephone, apps, device or monitoring systems, and web-based technologies. Most initiatives showed either similar or better health and behavioral outcomes compared with in-person care. The key barriers for wider adoption were physical, cognitive, or sensory impairment in older adults and staffing issues, legislative issues, and a lack of motivation among providers.

Conclusions
Virtual care is a viable model of care to address a wide range of health conditions among older adults in Australia. More embedded and integrative evaluations are needed to ensure that virtually enabled care can be used more widely by older Australians and health care providers.