bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mouse model of insomnia with sleep apnea
Authors: Masubuchi Satoru , Yano Takako , Komatsu Kouji , Ikegami Keisuke , Nakamura Wataru , Ota Akinobu , Karnan Sivasundaram , Takeuchi Kosei , Hosokawa Yoshitaka , Todo Takeshi , Shiomi Toshiaki 
Published: August 2022

AbstractObstructive sleep apnea (OSA) patients are exposed to nighttime hypoxia during sleep by intermittent airway closure and feel daytime strong sleepiness. Strangely, insomnia co-occur in some OSA patients, which is called co-morbid insomnia and sleep apnea (COMISA). Here, we show activity responses to daytime hypoxia (DHx) in nocturnal mice were comparable to daytime sleepiness and co-occurring nighttime insomnia in COMISA. DHx reduced activity in active phase (AP) and increased following activity in activity ending phase (AEP). This down-and-up activity response (DUR) by DHx was also observed in molecular clock deficient Cry1 and Cry2 double knockout mice (CryDKO) expressing nighttime activity rise under light-dark cycle (LD) and not observed in arrhythmic CryDKO under constant darkness (DD). When daytime timing hypoxia was exposed at transition from LD to DD, about every 6 h down and up and down wavelike activity responses appeared in arrhythmic CryDKO. Results indicate this wavelike response and AP activity overlap and cause DUR in rhythmic mice. DHx increased plasma corticosterone and this increase antagonized AP activity reduction by DHx. DHx reduced forebrain adenosine and morning adenosine inhibition by caffeine induced DUR. Adenosine inhibition by caffeine or istradefylline at transition from LD to DD induced wavelike response in CryDKO. It is possible that wavelike response is damped oscillation because, interestingly, chronic caffeine treatment induced circasemidian and/or circadian activity rhythms in arrhythmic CryDKO. Evening caffeine attenuated DUR by DHx, which suggested adenosine inhibition chronotherapy may improve OSA/COMISA symptoms. Our animal model will be useful to understand COMISA.SignificanceObstructive sleep apnea patients (OSA) are exposed to nighttime hypoxia during sleep. OSA feels daytime strong sleepiness and increases risk of many diseases. Insomnia occurs in not a few OSA, which is called comorbid insomnia and sleep apnea (COMISA). We show here a mouse model of COMISA. In mice, daytime hypoxia exposure induced following down and up activity response (DUR), activity reduction in active phase and increase in activity ending phase, which corresponded to sleepiness and insomnia in COMISA. We found DUR was clock gene independent and might be driven by circasemidian system. Glucocorticoid and forebrain adenosine response were involved in DUR. Caffeine chronotherapy was effective in DUR. Our model may be useful to understand COMISA.
Mass spectrometry imaging reveals early metabolic priming of cell lineage in differentiating human induced pluripotent stem cells
Authors: Nikitina Arina A. , Van Grouw Alexandria , Roysam Tanya , Huang Danning , Fernández Facundo M. , Kemp Melissa L. 
Published: August 2022

AbstractInduced pluripotent stem cells (iPSCs) hold great promise in regenerative medicine; however, few algorithms of quality control at the earliest stages of differentiation have been established. Despite lipids having known functions in cell signaling, their role in pluripotency maintenance and lineage specification is underexplored. We investigated changes in iPSC lipid profiles during initial loss of pluripotency over the course of spontaneous differentiation using co-registration of confocal microscopy and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging. We identified lipids that are highly informative of the temporal stage of the differentiation and can reveal lineage bifurcation occurring metabolically. Several phosphatidylinositol species emerged from machine learning analysis as early metabolic markers of pluripotency loss, preceding changes in Oct4. Manipulation of phospholipids via PI 3-kinase inhibition during differentiation manifested in spatial reorganization of the colony and elevated expression of NCAM-1. In addition, continuous inhibition of phosphatidylethanolamine N-methyltransferase during differentiation resulted in increased pluripotency maintenance. Our machine learning analysis highlights the predictive power of metabolic metrics for evaluating lineage specification in the initial stages of spontaneous iPSC differentiation.
On-grid labeling method for freeze-fracture replicas
Authors: Osakada Hiroko , Fujimoto Toyoshi 
Published: August 2022

AbstractSodium dodecyl sulfate–treated freeze-fracture replica labeling (SDS-FRL) is an electron microscopic (EM) method that can define the two-dimensional distribution of membrane proteins and lipids in a quantitative manner. Despite its unsurpassed merit, SDS-FRL has been adopted in a limited number of labs, probably because it requires a laborious labeling process as well as equipment and technique for freeze-fracture. Here, we present a method that reduces the manual labor significantly by mounting freeze-fracture replicas on EM grids prior to labeling. This was made possible by the discovery that freeze-fracture replicas invariably adhere to the carbon-coated formvar membrane with their platinum-carbon side, ensuring that the membrane molecules retained in replicas are accessible to labeling solutions. The replicas mounted on EM grids can be stored dry until labeling, checked by light microscopy before labeling, and labeled in the same manner as tissue sections. This on-grid method will make SDS-FRL easier to access for many researchers.
syntenet: an R/Bioconductor package for the inference and analysis of synteny networks
Authors: Almeida-Silva Fabricio , Zhao Tao , Ullrich Kristian K. , Schranz M. Eric , Van de Peer Yves 
Published: August 2022

ABSTRACTInterpreting and visualizing synteny relationships across several genomes is a challenging task. We previously proposed a network-based approach for better visualization and interpretation of large-scale microsynteny analyses. Here, we present syntenet, an R package to infer and analyze synteny networks from whole-genome protein sequence data. The package offers a simple and complete framework, including data preprocessing, synteny detection and network inference, network clustering and phylogenomic profiling, and microsynteny-based phylogeny inference. Graphical functions are also available to create publication-ready plots. Synteny networks inferred with syntenet can highlight taxon-specific gene clusters that likely contributed to the evolution of important traits, and microsynteny-based phylogenies can help resolve phylogenetic relationships under debate.Availability and ImplementationThe package is available on Bioconductor (https://bioconductor.org/packages/syntenet), and the source code is available on a GitHub repository (https://github.com/almeidasilvaf/syntenet).
Modulation of xanthophyll cycle impacts biomass productivity in the marine microalgaNannochloropsis
Authors: Perin Giorgio , Bellan Alessandra , Lyska Dagmar , Niyogi Krishna K. , Morosinotto Tomas 
Published: August 2022

AbstractLife on earth depends on photosynthetic primary producers that exploit sunlight to fix CO2into biomass. Approximately half of global primary production is associated with microalgae living in aquatic environments. Microalgae also represent a promising source of biomass to complement crop cultivation, and they could contribute to the development of a more sustainable bioeconomy. Photosynthetic organisms evolved multiple mechanisms involved in the regulation of photosynthesis to respond to highly variable environmental conditions. While essential to avoid photodamage, regulation of photosynthesis results in dissipation of absorbed light energy, generating a complex trade-off between protection from stress and light-use efficiency. This work investigates the impact of the xanthophyll cycle, the light-induced reversible conversion of violaxanthin into zeaxanthin, on the protection from excess light and on biomass productivity in the marine microalgae of the genusNannochloropsis. Zeaxanthin is shown to have an essential role in protection from excess light, contributing to the induction of Non-Photochemical Quenching and scavenging of reactive oxygen species. On the other hand, the overexpression of Zeaxanthin Epoxidase, enables a faster re-conversion of zeaxanthin to violaxanthin that is shown to be advantageous for biomass productivity in dense cultures in photobioreactors. These results demonstrate that zeaxanthin accumulation is critical to respond to strong illumination, but it may lead to unnecessary energy losses in light-limiting conditions, and accelerating its re-conversion to violaxanthin provides an advantage for biomass productivity in microalgae.Significance StatementThis work investigates the impact of the xanthophyll cycle in marine microalgae on the trade-off between photoprotection and light-use efficiency. Our results demonstrate that whilst zeaxanthin is essential for photoprotection upon exposure to strong illumination, it leads to unnecessary energy losses in light-limiting conditions and thus accelerating its re-conversion to violaxanthin provides an advantage for biomass productivity in microalgae.
Selecting better biocatalysts by complementing recoded bacteria
Authors: Rubini Rudy , Jansen Suzanne C. , Beekhuis Houdijn , Rozeboom Henriëtte J. , Mayer Clemens 
Published: August 2022

AbstractIn vivo selections are powerful tools for the directed evolution of enzymes. However, the need to link enzymatic activity to cellular survival makes selections for enzymes that do not fulfill a metabolic function challenging. Here, we present an in vivo selection strategy that leverages recoded organisms addicted to non-canonical amino acids (ncAAs) to evolve biocatalysts that can provide these building blocks from synthetic precursors. We exemplify our platform by engineering carbamoylases that display catalytic efficiencies more than five orders of magnitude higher than those observed for the wild-type enzyme for ncAA-precursors. As growth rates of bacteria under selective conditions correlate with enzymatic activity, we were able to elicit improved variants from populations by performing serial passaging. By requiring minimal human intervention and no specialized equipment, we surmise that our strategy will become a versatile tool for the in vivo directed evolution of diverse biocatalysts.
Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog Pathway Inhibitor-1
Authors: Bagka Meropi , Choi Hyeonyi , Heritier Margaux , Scapozza Leonardo , Wu Yibo , Hoogendoorn Sascha 
Published: August 2022

AbstractTarget deconvolution of small molecule hits from phenotypic screens presents a major challenge. Illustrative of these are the many screens that have been conducted to find inhibitors for the Hedgehog (Hh) signaling pathway – a major developmental pathway with many implications in health and disease - with many hits but very few identified cellular targets. We here present a strategy for target identification based on Proteolysis-Targeting Chimeras (PROTACs), combined with label-free quantitative proteomics. We developed a PROTAC based on the downstream Hedgehog Pathway Inhibitor-1 (HPI-1), a phenotypic screen hit with unknown cellular target. Using our Hedgehog Pathway PROTAC (HPP) we identified and validated BET bromodomains to be the cellular targets of HPI-1. Furthermore, we found that HPP-9 has a unique mechanism of action as a long-acting Hh pathway inhibitor through prolonged BET bromodomain degradation. Collectively, we provide a powerful PROTAC-based approach for target deconvolution, that has answered the longstanding question of the cellular target of HPI-1 and yielded the first PROTAC that acts on the Hh pathway.
Polymer brush bilayer under stationary shear: A joint DFT, scaling theory and MD
Authors: Edwards (Majid Farzin) Mike John 
Published: August 2022

AbstractThe problem of polymer brush bilayer under stationary shear is studied by using the DFT, the scaling theory and MD simulations. Both theory and simulations confirm that the shear stress follows the universal power lawfor the brush bilayers with interpenetration and in the absence of the interpenetration, the shear stress scales linearly with the shear rate. It is also revealed that the presence of explicit solvent molecules prevents the brushes to form and interpenetration zone and therefore with explicit solvents the shear stress scales linearly with shear rate. Therefore, this study strongly confirms that there is no sublinear regime in the world of polymer brush bilayer, neither by solvents nor by hydrodynamic effects. As long as there is an interpenetration zone, the superlinear regime dominates and in the absence of the interpenetration zone the linear regime dominates. Therefore, polymer brushes are not a good candidate for lubrication and all works suggesting that this system is a super lubricant are completely wrong.
“On duty in pursuit of the natives”: accounting and truth-telling about Australia's frontier wars
Authors: Sutton Nicole 
Published: August 2022

PurposeThis paper considers how archival accounting records may support truth-telling about past atrocities during Australia's frontier wars.Design/methodology/approachThe study examines two colonial accounting records – military muster payrolls and the ledger statements of a local tax fund – used during the British's punitive expeditions against the Aboriginal peoples of Sydney in 1816.FindingsThe accounting records reveal new information about the full scale of the campaign, the degree to which the violence was formally endorsed and acts of Aboriginal resistance. However, much of the human toll of the campaign remains obscured by the highly structured, monetary lens of financial records authored and archived by the British colonial regime.Social implicationsAustralia's First Nations have called for greater truth-telling about the frontier wars to enable meaningful reconciliation and political recognition of Indigenous sovereignty. This study highlights the potential role of accounting records as a resource for contemporary truth-telling processes.Originality/valueThe study contributes to the literature about the dark history of accounting by explicating genre features in the content, form and context of archival accounting records, which can both render past atrocities more visible as well as perpetrate invisibilities, ambiguities and silences.
Habitual Facebook use as a prognosticator for life satisfaction and psychological well-being: social safeness as a moderator
Authors: Maziriri Eugine Tafadzwa , Nyagadza Brighton , Mapuranga Miston , Maramura Tafadzwa Clementine 
Published: August 2022

PurposeThis study aims to examine the impact of habitual Facebook use (HFU) on life satisfaction and psychological well-being. In addition, the study examined the impact of life satisfaction on psychological well-being. Moreover, the study investigates the impact of social safeness in moderating the relationship between HFU and life satisfaction.Design/methodology/approachThe study employed a quantitative survey design, using a sample of 261 Generation Y students based in Mthatha, in the Eastern Cape Province of South Africa. Scales for data collection were operationalized from prior studies. The collected data were analyzed using structural equation modeling.FindingsThe study’s results disclosed that HFU positively and significantly impacts life satisfaction and psychological well-being. In addition, life satisfaction positively and significantly impacted psychological well-being. Moreover, the results showed that social safeness had a positive and significant moderating effect on the nexus between HFU and life satisfaction.Originality/valueThis study contributes to understanding HFU as a precursor to life satisfaction and psychological well-being among Generation Y students. Also, evaluating the moderating effect of social safeness contributes to a more thorough understanding of the link between HFU and life satisfaction. Furthermore, this research aims to add to the body of knowledge in Africa’s communication psychology and social media literature, a field that has received little academic attention in developing countries.
Exploring factors influencing impulse buying in live streaming shopping: a stimulus-organism-response (SOR) perspective
Authors: Lin Shu-Chiung , Tseng Hsiao-Ting , Shirazi Farid , Hajli Nick , Tsai Pei-Tzu 
Published: August 2022

PurposeBased on the stimulus-organism-response theory, this research constructs the influence of the stimulus factors of the live-streaming shopping environment on consumers' psychological situation. It then produces the research model of impulsive purchase intention.Design/methodology/approachIn this study, the online questionnaire survey method was used to survey users who participated in live-streaming shopping, and a total of 335 valid questionnaires were collected. Then SPSS and SmartPLS were used for data empirical evaluation and hypotheses test.FindingsResearch results show that demand, convenience, interactivity, and playfulness are positively stimulating consumers' perceived enjoyment. And their perceived enjoyment directly drives their intention of impulsive purchase.Practical implicationsThe choice of the live streaming platform, the design of the interactive interface, and the design of the shopping process are all factors that the streamer must carefully consider. The results of this study can be used as a reference for the development of live-streaming shopping and provide the industry with an understanding of the main factors that affect users' live streaming and impulsive purchases to plan an effective live streaming platform and content.Originality/value“E-commerce live streaming” is regarded as the latest trend of e-commerce, and impulse buying is regarded as a key factor in the success of transactions. This research has developed factors that influence impulsive purchases after watching live streaming based on the SOR theory.
A self-interested gesture? Corporate charitable giving in response to government fiscal pressure
Authors: Lu Shengfeng , Chen Sixia , Cang Yongtao , San Ziyao 
Published: August 2022

PurposeThis study examines whether and how government fiscal pressure influences corporate charitable giving (CCG).Design/methodology/approachThe authors exploit sub-national tax revenue sharing changes as exogenous variations to government’s fiscal pressure at the city level and then construct a quasi difference-in-differences (DiD) model to conduct the analysis based on a sample that consists of 14,168 firm-year observations in China during the period of 2003 to 2012.FindingsThe authors found that firms increase charitable donations when local governments face higher fiscal pressure. Such effects are more pronounced for firms that have stronger demand for political connectedness in the sample period. Furthermore, this study’s findings suggest that the timing strategy of donating helps firms to lower the effective tax rate and to build stronger political connections. In addition, donating firms outperform non-donating firms in terms of bank loan access and market reputation.Originality/valueThe authors contribute to at least three lines of literature: first, extend the understanding of timing strategies of corporate charitable behaviors; second, contribute to the literature studying the “crowd out” effect between government-provided charitable funds and private donations; finally, contribute to the emerging literature exploring the financial interests associated with corporate donation strategy (Claessenset al., 2008; Cullet al., 2015).
Reporting on Sustainable Development Goals in the European Union: what drives companies’ decisions?
Authors: Krasodomska Joanna , Zieniuk Paweł , Kostrzewska Jadwiga 
Published: August 2022

Purpose
This paper aims to identify the changes in the share of large public interest entities (PIEs) in European Union (EU) Member States providing Sustainable Development Goal (SDG) reporting prior to (2017) and after (2019) the implementation of Directive 2014/95/EU and the factors that influence their decisions to provide SDG reporting in 2019.

Design/methodology/approach
The authors use the multilevel theory of social change in organizations as the theoretical background. The sample consists of 341 PIEs based in the EU Member States, for which reports published in 2017 and 2019 are available in the global reporting initiative sustainability disclosure database. The authors analyzed the data using the statistical significance test of equal proportions and the logistic regression model.

Findings
The study findings allow to identify a significant positive change in the share of companies providing a reference to SDGs in 2019 compared with 2017. The research confirms that companies’ engagement in United Nations Global Compact and previous experience in sustainability reporting positively influences the decision to report on SDGs in 2019. Contrary to the expectations, industry, size, SDG implementation score, future orientation of government and corporate governance score do not seem to be relevant factors influencing PIEs’ disclosures.

Originality/value
The paper adds to the understanding of the differences in SDG reporting within the EU, which is seen as a frontrunner in implementing the 2030 Agenda and the SDGs.
Non-technical skills and students' overconfidence in accounting
Authors: Arquero Jose Luis , Fernandez-Polvillo Carmen , Hassall Trevor 
Published: August 2022

PurposeDespite the institutional calls to include the development of non-technical skills as objectives in accounting curriculum and the attempts to do so, a gap between the level of skills exhibited by graduates and those needed to succeed as a professional is still perceived. One of the possible causes could be students' overconfidence, defined as a very optimistic assessment of their own abilities. The main objective of the paper is to assess the existence of overconfidence.Design/methodology/approachTwo samples, students and employers were surveyed regarding the exhibited level of accounting graduates in a set of 22 non-technical skills, highlighted as relevant in the literature. This enabled a comparison of the opinions of employers with the perceptions of students concerning the demonstrated level of such skills.FindingsThe results of this study support the existence of students' overconfidence. In all the skills students score their ability higher than employers do with those differences being statistically significant in 21 out of 22 skills. Employers who are in closer contact with entry level accountants perceive even lower exhibited skills levels in graduates.Research limitations/implicationsOverconfident students would be less motivated to actively participate in activities designed to improve skills resulting in underachievement and in lower performance. This low performance in highly valued skills could potentially harm their employability.Originality/valueAlthough the literature focussing on non-technical skills in accounting is prolific there are few papers comparing the views of employers and students, and there are no previous studies focussing on overconfidence.