bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fine mapping and cloning of the major seed protein quantitative trait loci on soybean chromosome 20
Authors: Fliege Christina E. , Ward Russell A. , Vogel Pamela , Nguyen Hanh , Quach Truyen , Guo Ming , Viana João Paulo Gomes , dos Santos Lucas Borges , Specht James E. , Clemente Tom E. , Hudson Matthew E. , Diers Brian W. 
Published: January 2022

SUMMARYSoybean [Glycine max (L.) Merr.] is a unique crop species because it has high levels of both protein and oil in its seed. Of the many quantitative trait loci (QTL) controlling soybean seed protein content, alleles of the cqSeed protein‐003 QTL on chromosome 20 exert the greatest additive effect. The high‐protein allele exists in both cultivated and wild soybean (Glycine soja Siebold & Zucc.) germplasm. Our objective was to fine map this QTL to enable positional‐based cloning of its underlying causative gene(s). Fine mapping was achieved by developing and testing a series of populations in which the chromosomal region surrounding the segregating high‐ versus low‐protein alleles was gradually narrowed, using marker‐based detection of recombinant events. The resultant 77.8 kb interval was directly sequenced from a G. soja source and compared with the reference genome to identify structural and sequence polymorphisms. An insertion/deletion variant detected in Glyma.20G85100 was found to have near‐perfect +/− concordance with high/low‐protein allele genotypes inferred for this QTL in parents of published mapping populations. The indel structure was concordant with an evolutionarily recent insertion of a TIR transposon into the gene in the low‐protein lineage. Seed protein was significantly greater in soybean expressing an RNAi hairpin downregulation element in two independent events relative to control null segregant lineages. We conclude that a transposon insertion within the CCT domain protein encoded by the Glyma.20G85100 gene accounts for the high/low seed protein alleles of the cqSeed protein‐003 QTL.
Long-term outcomes and prognostic factors in patients with treated spinal dural arteriovenous fistulas: a prospective cohort study
Authors: Yang Chengbin , Ma Yongjie , Tian An , Yu Jiaxing , Chen Sichang , Peng Chao , Yang Kun , Li Guilin , He Chuan , Ye Ming , Hong Tao , Bian Lisong , Wang Zhichao , Ling Feng , Zhang Hongqi 
Published: January 2022

ObjectiveTo define the pattern of long-term clinical outcomes and prognostic factors in patients with spinal dural arteriovenous fistulas (SDAVFs).DesginProspective cohort study based on constantly recruiting patients with SDAVFs in two medical centres in China.SettingPatients with SDAVFs were recruited consecutively between March 2013 and December 2014 in two referral centres.ParticipantsA prospective cohort of 94 patients with SDAVFs was included in this study, and 86 patients (mean age 53.0 years, 71 men) completed the study. Patients who had previously undergone endovascular or neurosurgical treatment or had neurological dysfunction caused by other diseases or refused treatment were excluded.InterventionsAll patients underwent neurosurgery or endovascular embolisation. These patients were evaluated with the modified Aminoff and Logue’s Scale (mALS) 1 day before and 3, 6, 12 and 72 months after treatments.ResultsThe duration of symptoms ranged from 0.5 to 66 months (average 12.8 months). The location of SDAVFs was as follows: 33.7% above T7, 50.0% between/include T7 and T12% and 16.3% below T12. 75 patients (87.2%) underwent neurosurgical treatment, and 9 patients (10.5%) underwent endovascular treatment. 58 patients (67.4%) exhibited an improvement in mALS of one point or greater at 72 months. Patients with less disability were more likely to improve at 72 months (p<0.05). 48 patients (55.8%) showed deterioration at 72 months compared with 12 months. 61% of the patients suffered numbness, and 22% had pain before treatment. However, 81% of patients had numbness, and 28% had pain after treatment. This deterioration was related to 1-year mALS and age.ConclusionNearly two-thirds of the patients experienced clinical improvement at 72 months, and preoperative (1 day before treatment) mALS was the strongest predictor of clinical improvement. However, 55.8% of patients showed deterioration after temporary recovery. All patients with SDAVFs should accept treatment as soon as possible.
ACCEPTANCE: protocol for a feasibility study of a multicomponent physical activity intervention following treatment for cervical cancer
Authors: Millet Nessa , McDermott Hilary J , Munir Fehmidah , Edwardson Charlotte L , Moss Esther L 
Published: January 2022

IntroductionCervical cancer treatment can have life changing sequelae and be associated with poor short-term and long-term quality of life. Physical activity (PA; that is, bodily movement) is known to improve health outcomes and quality of life for cancer survivors, both physically and psychologically. To date, no interventions to increase PA following cervical cancer have been evaluated. This study aims to (1) determine the feasibility of conducting a PA intervention after cervical cancer and (2) to explore the acceptability of the programme and evaluation measures.Methods and analysisThe design is a pre study and post study design. Thirty participants aged between 18 and 60 years from the Midlands region, UK, who have completed primary treatment for cervical cancer at least 6 months previously and do not meet the national PA guidelines will be recruited. Identification of potential participants will take place through the University Hospitals of Leicester National Health Service (NHS) Trust. Participants will receive an intervention focused on increasing PA through the provision of education, action planning, goal setting, problem solving and self-monitoring of PA behaviour, particularly steps per day. Device assessed PA and questionnaires will be completed at baseline, week 6, week 12 and week 24. Feasibility will be assessed in terms of recruitment, retention, attrition, completion of measures and intervention compliance, for which specific feasibility criteria have been established. The process evaluation will explore the experiences and acceptability of the intervention components and evaluation measures.Ethics and disseminationEthical approval has been granted by the West of Scotland Research Ethics Committee 1 for this study. Results will inform intervention refinement for the design of a definitive pilot trial. These results will be disseminated via peer-reviewed publications and international conferences while input from a patient and public involvement (PPI) group will inform effective ways to circulate results among the wider community.Trial registration numberISRCTN16349793, Registered 30 September 2020.
Low length/weight growth trajectories of early-term infants during the first year: evidence from a longitudinal study in China
Authors: Chen Kai , Song Lulu , Liu Bingqing , Wu Mingyang , Liu Yunyun , Wang Lulin , Bi Jianing , Liu Qing , Zhang Yiming , Tang Zezhong , Wang Youjie , Yang Rong 
Published: January 2022

ObjectiveTo identify common length, weight and body mass index (BMI) growth trajectories of term infants during infancy, and to determine their association with early-term infants.DesignProspective longitudinal study.SettingWuhan, China.PatientsA total of 4308 term infants (born at 37–41 weeks of gestation) were included. All term infants were single live birth with no defects and birth weight ≥2500 g, and their mothers were permanent residents of Wuhan for more than 2 years. After excluding 887 infants, a total of 3421 term infants (1028 early-term infants born at 37–38 weeks of gestation and 2393 full-term infants born at 39–41 weeks of gestation) entered the statistical analysis stage.Main outcome measuresPatterns of length, weight and BMI growth trajectories by using group-based trajectory modelling.ResultsThree distinct physical growth trajectories were identified as follows: length: low stable (1056, 30.9%), moderate stable (1887, 55.2%) and high increasing (477, 13.9%); weight: low stable (1031, 30.1%), moderate stable (1884, 55.1%) and high increasing (505, 14.8%); BMI: low stable (689, 20.1%), moderate stable (2167, 63.4%) and high increasing (564, 16.5%). Compared with the full-term infants, early-term infants were more likely to remain at low-stable trajectory in length (OR: 1.40; 95% CI: 1.19 to 1.66) and weight (OR:1.29; 95% CI: 1.09 to 1.53). These associations were still statistically significant after adjusting potential confounders and were more evident among girls in the stratified analysis. There was no statistical association between BMI trajectory patterns and gestational age categories.ConclusionOur results suggested the heterogeneity of term infants existed in length, weight and BMI growth trajectories of early childhood. Compared with full-term birth, early-term birth was related to low length and weight trajectories rather than BMI trajectory. Further research is needed to evaluate the duration of these low trajectories and their possible long-term health effects.
Service use, clinical outcomes and user experience associated with urgent care services that use telephone-based digital triage: a systematic review
Authors: Sexton Vanashree , Dale Jeremy , Bryce Carol , Barry James , Sellers Elizabeth , Atherton Helen 
Published: January 2022

ObjectiveTo evaluate service use, clinical outcomes and user experience related to telephone-based digital triage in urgent care.DesignSystematic review and narrative synthesis.Data sourcesMedline, Embase, CINAHL, Web of Science and Scopus were searched for literature published between 1 March 2000 and 1 April 2020.Eligibility criteria for selecting studiesStudies of any design investigating patterns of triage advice, wider service use, clinical outcomes and user experience relating to telephone based digital triage in urgent care.Data extraction and synthesisTwo reviewers extracted data and conducted quality assessments using the mixed methods appraisal tool. Narrative synthesis was used to analyse findings.ResultsThirty-one studies were included, with the majority being UK based; most investigated nurse-led digital triage (n=26). Eight evaluated the impact on wider healthcare service use following digital triage implementation, typically reporting reduction or no change in service use. Six investigated patient level service use, showing mixed findings relating to patients’ adherence with triage advice. Evaluation of clinical outcomes was limited. Four studies reported on hospitalisation rates of digitally triaged patients and highlighted potential triage errors where patients appeared to have not been given sufficiently high urgency advice. Overall, service users reported high levels of satisfaction, in studies of both clinician and non-clinician led digital triage, but with some dissatisfaction over the relevance and number of triage questions.ConclusionsFurther research is needed into patient level service use, including patients’ adherence with triage advice and how this influences subsequent use of services. Further evaluation of clinical outcomes using larger datasets and comparison of different digital triage systems is needed to explore consistency and safety. The safety and effectiveness of non-clinician led digital triage also needs evaluation. Such evidence should contribute to improvement of digital triage tools and service delivery.PROSPERO registration numberCRD42020178500.
Prospective longitudinal study of ‘Sleepless in Lockdown’: unpacking differences in sleep loss during the coronavirus pandemic in the UK
Authors: Falkingham Jane C , Evandrou Maria , Qin Min , Vlachantoni Athina 
Published: January 2022

ObjectivesCOVID-19 is having a disproportionate impact on Black, Asian and minority ethnic (BAME) groups and women. Concern over direct and indirect effects may also impact on sleep. We explore the levels and social determinants of self-reported sleep loss among the UK population during the pandemic, focusing on ethnic and gender disparities.SettingThis prospective longitudinal study analysed data from seven waves of the Understanding Society: COVID-19 Study collected from April 2020 to January 2021 linked to prepandemic data from the 2019 mainstage interviews, providing baseline information about the respondents prior to the pandemic.ParticipantsThe analytical sample included 8163 respondents aged 16 and above who took part in all seven waves with full information on sleep loss, defined as experiencing ‘rather more’ or ‘much more’ than usual sleep loss due to worry, providing 57 141 observations.Primary outcome measuresSelf-reported sleep loss. Mixed-effects regression models were fitted to consider within-individual and between-individual differences.ResultsWomen were more likely to report sleep loss than men (OR 2.1, 95% CI 1.9 to 2.4) over the 10-month period. Being female, having young children, perceived financial difficulties and COVID-19 symptoms were all predictive of sleep loss. Once these covariates were controlled for, the bivariate relationship between ethnicity and sleep loss (1.4, 95% CI 1.6 to 2.4) was reversed (0.7, 95% CI 0.5 to 0.8). Moreover, the strength of the association between gender and ethnicity and the risk of sleep loss varied over time, being weaker among women in July (0.6, 95% CI 0.5 to 0.7), September (0.7, 95% CI 0.6 to 0.8), November (0.8, 95% CI 0.7 to 1.0) and January 2021 (0.8, 95% CI 0.7 to 0.9) compared with April 2020, but positively stronger among BAME individuals in May (1.4, 95% CI 1.0 to 2.1), weaker only in September (0.7, 95% CI 0.5 to 1.0).ConclusionsThe pandemic has widened sleep deprivation disparities, with women with young children, COVID-19 infection and BAME individuals experiencing sleep loss, which may adversely affect their mental and physical health.
Review of novel therapeutics in cardiac arrest (ReNTICA): systematic review protocol
Authors: Murphy Travis W , Snipes Garrett , Chowdhury Muhammad Abdul Baker , McCall-Wright Patti , Aleong Elizabeth , Taylor Noelle , Messina Maiya-Mari , Carrazana Gabriela , Maciel Carolina B , Becker Torben K 
Published: January 2022

IntroductionCardiac arrest remains a common and devastating cause of death and disability worldwide. While targeted temperature management has become standard of care to improve functional neurologic outcome, few pharmacologic interventions have shown similar promise.Methods/analysisThis systematic review will focus on prospective human studies from 2015 to 2020 available in PubMed, Web of Science and EMBASE with a primary focus on impact on functional neurologic outcome. Prospective studies that include pharmacologic agents given during or after cardiac arrest will be included. Study selection will be in keeping with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. If sufficient data involving a given agent are available, a meta-analysis will be conducted and compared with current evidence for therapies recommended in international practice guidelines.Ethics and disseminationFormal ethical approval will not be required as primary data will not be collected. The results will be disseminated through peer-reviewed publication, conference presentation and lay press.PROSPERO registration numberInternational Prospective Register for Systematic Reviews (CRD42021230216).
‘We have a plan for that’: a qualitative study of health system resilience through the perspective of health workers managing antenatal and childbirth services during floods in Cambodia
Authors: Saulnier Dell D , Thol Dawin , Por Ir , Hanson Claudia , von Schreeb Johan , Alvesson Helle Mölsted 
Published: January 2022

ObjectiveHealth system resilience can increase a system’s ability to deal with shocks like floods. Studying health systems that currently exhibit the capacity for resilience when shocked could enhance our understanding about what generates and influences resilience. This study aimed to generate empirical knowledge on health system resilience by exploring how public antenatal and childbirth health services in Cambodia have absorbed, adapted or transformed in response to seasonal and occasional floods.DesignA qualitative study using semi-structured interviews and thematic analysis and informed by the Dimensions of Resilience Governance framework.SettingPublic sector healthcare facilities and health departments in two districts exposed to flooding.ParticipantsTwenty-three public sector health professionals with experience providing or managing antenatal and birth services during recent flooding.ResultsThe theme ‘Collaboration across the system creates adaptability in the response’ reflects how collaboration and social relationships among providers, staff and the community have delineated boundaries for actions and decisions for services during floods. Floods were perceived as having a modest impact on health services. Knowing the boundaries on decision-making and having preparation and response plans let staff prepare and respond in a flexible yet stable way. The theme was derived from ideas of (1) seasonal floods as a minor strain on the system compared with persistent, system-wide organisational stresses the system already experiences, (2) the ability of the health services to adjust and adapt flood plans, (3) a shared purpose and working process during floods, (4) engagement at the local level to fulfil a professional duty to the community, and (5) creating relationships between health system levels and the community to enable flood response.ConclusionThe capacity to absorb and adapt to floods was seen among the public sector services. Strategies that enhance stability and flexibility may foster the capacity for health system resilience.
Sirolimus versus tacrolimus for systemic lupus erythematosus treatment: results from a real-world CSTAR cohort study
Authors: Jiang Nan , Li Mengtao , Zhang Hongfeng , Duan Xinwang , Li Xiaofeng , Fang Yongfei , Li Hongbin , Yang Pingting , Luo Hui , Wang Yanhong , Peng Liying , Zhao Jiuliang , Wu Chanyuan , Wang Qian , Tian Xinping , Zhao Yan , Zeng Xiaofeng 
Published: January 2022

ObjectiveThe effectiveness and safety of sirolimus for SLE treatment have been shown in some uncontrolled studies. However, a comparison of sirolimus with other classic immunosuppressants has not been reported. We conducted the study to compare the effectiveness and safety of sirolimus versus tacrolimus for SLE treatment.MethodsA real-world cohort study was conducted. Patients with clinically active SLE who were prescribed sirolimus or tacrolimus were enrolled. Propensity score matching was used to ensure equivalent disease conditions and background medications. SLE disease activity indices, serological parameters, steroid doses, modification of other immunosuppressants, renal effectiveness and adverse events were compared between the two groups at 3-month, 6-month, 9-month and 12-month follow-up visits.ResultsData from 52 patients in each of the sirolimus and tacrolimus groups were analysed. Indices regarding the effectiveness of sirolimus, including Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) scores, physician’s global assessment (PhGA) scores, and proportion of patients with SLEDAI-2K reduction of ≥4 and PhGA increase of <0.3, were equivalent to those of tacrolimus at all follow-up timepoints (all p≥0.05). Greater improvements in complement levels were observed in the sirolimus group at 3 and 6 months. Higher percentages of patients with prednisone doses ≤7.5 mg/day were observed in the sirolimus group at all timepoints. Seventeen adverse events in the sirolimus group were recorded. None was severe or led to drug discontinuation.ConclusionsOverall, sirolimus was as effective as tacrolimus in the treatment of SLE. Sirolimus had better effects on serological improvement and glucocorticoid tapering. Sirolimus was well tolerated in patients with SLE.
Analysis of fabrics and metal threads from two Andalusian liturgical vestments from the seventeenth and eighteenth centuries: a multitechnical approach
Authors: Durán Adrián , Pérez-Rodríguez José Luis 
Published: January 2022

AbstractThis manuscript provided one of the first scientific studies performed on ancient Spanish liturgical vestments by using a multitechnical physicochemical approach. The interlining and lining fabrics were done by cotton and the core of threads by silk. Silver, gold and little amounts of copper were detected. The highest values of gold were found in the metal threads of the cope and the gallon of the chasuble. Two layers were identified: the internal composed by silver and the external by silver and gold. The fibers were proved to be degraded and also the surface of the metal, where scratches and silver-based compounds were detected due to environmental and anthropogenic actions. Indigo was employed as dye in the blue and green fabrics. Wax was detected in the yellow fibers, although other dyes could not be discarded. The materials used in the restoration processes were of minor quality.
Redescription of Nothobomolochus triceros (Bassett-Smith, 1898) (Copepoda, Cyclopoida, Bomolochidae), with descriptions of the male and copepodids V-II of the species
Authors: Izawa Kunihiko 
Published: January 2022

Abstract
Nothobomolochus triceros (Bassett-Smith, 1898), based on specimens recovered from the original host Pampus argenteus (Euphrasen, 1788) in Japan, are redescribed, and the male, both sexes of copepodids V and IV, and sex undetermined copepodids III and II of the species are described for the first time. The maxilliped is sexually dimorphic only in the adult in Bomolochidae, on the other hand, the rostral plate, antennule, and legs 1, 4, and 5 are sexually dimorphic in Nothobomolochus throughout the adult and copepodids V-IV. Sex is as yet undetermined in copepodids III and II in the genus.
New record of the swimming crab Charybdis (Archias) hongkongensis Shen, 1934 (Brachyura, Portunidae) from India
Authors: Prema M. , Ravichandran S. , Sivakumar V. K. 
Published: January 2022

Abstract
Charybdis (Archias) hongkongensis Shen, 1934 was collected from the Indian coast of the Bay of Bengal and this is the first record from Indian waters. The distinct key characters of this species are: a relatively more produced last antero-lateral tooth, strongly convergent postero-lateral margins, and a smooth, rounded, reduced apex of the male first gonopod. This is the ninth member of Charybdis (Archias) Alcock, 1899 from Indian waters. The species is diagnosed and figured herein.
Sound and Ritualistic Language in Charles Williams’ War in Heaven
Authors: Grant Gwen 
Published: January 2022

Abstract
This study uncovers a link between sound patterns and ritualistic language in Charles Williams’ novels through an analysis of the relationship between type of sound and content. The study focuses on War in Heaven with a view to conducting a preliminary exploration into this link, and establishing possibilities for future research. Like Williams’ other novels, War in Heaven is saturated with the symbolism and ritual practices he learned in The Fellowship of the Rosy Cross and, potentially, the Hermetic Order of the Golden Dawn. Williams’ experimentation with sound to convey his experience of ritual is explored through the framework of Roman Jakobson’s “Poetic Function”, to establish how Williams may have intended sound to contribute to the experience of the reader. Using a data driven approach, the study explores how sound patterns work with ritualistic content across War in Heaven, discovering a link between fricative sounds and ritualistic events.