bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knockdown of FOXA1 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the ERK1/2 signalling pathway
Authors: Li Lijun , Wang Yibo , Wang Zhongxiang , Xue Deting , Dai Chengxin , Gao Xiang , Ma Jianfei , Hang Kai , Pan Zhijun 
Published: September 2022

Abstract
Background
The available therapeutic options for large bone defects remain extremely limited, requiring new strategies to accelerate bone healing. Genetically modified bone mesenchymal stem cells (BMSCs) with enhanced osteogenic capacity are recognised as one of the most promising treatments for bone defects.

Methods
We performed differential expression analysis of miRNAs between human BMSCs (hBMSCs) and human dental pulp stem cells (hDPSCs) to identify osteogenic differentiation-related microRNAs (miRNAs). Furthermore, we identified shared osteogenic differentiation-related miRNAs and constructed an miRNA-transcription network. The Forkhead box protein A1 (FOXA1) knockdown strategy with a lentiviral vector was used to explore the role of FOXA1 in the osteogenic differentiation of MSCs. Cell Counting Kit-8 was used to determine the effect of the knockdown of FOXA1 on hBMSC proliferation; real-time quantitative reverse transcription PCR (qRT-PCR) and western blotting were used to investigate target genes and proteins; and alkaline phosphatase (ALP) staining and Alizarin Red staining (ARS) were used to assess ALP activity and mineral deposition, respectively. Finally, a mouse model of femoral defects was established in vivo, and histological evaluation and radiographic analysis were performed to verify the therapeutic effects of FOXA1 knockdown on bone healing.

Results
We identified 22 shared and differentially expressed miRNAs between hDPSC and hBMSC, 19 of which were downregulated in osteogenically induced samples. The miRNA-transcription factor interaction network showed that FOXA1 is the most significant and novel osteogenic differentiation biomarker among more than 300 transcription factors that is directly targeted by 12 miRNAs. FOXA1 knockdown significantly promoted hBMSC osteo-specific genes and increased mineral deposits in vitro. In addition, p-ERK1/2 levels were upregulated by FOXA1 silencing. Moreover, the increased osteogenic differentiation of FOXA1 knockdown hBMSCs was partially rescued by the addition of ERK1/2 signalling inhibitors. In a mouse model of femoral defects, a sheet of FOXA1-silencing BMSCs improved bone healing, as detected by microcomputed tomography and histological evaluation.

Conclusion
These findings collectively demonstrate that FOXA1 silencing promotes the osteogenic differentiation of BMSCs via the ERK1/2 signalling pathway, and silencing FOXA1 in vivo effectively promotes bone healing, suggesting that FOXA1 may be a novel target for bone healing.
Determining the optimal stage for cryopreservation of human embryonic stem cell-derived retinal pigment epithelial cells
Authors: Zhang Ting , Huang Xianyu , Liu Sujun , Bai Xinyue , Zhu Xinyue , Clegg Dennis O. , Jiang Mei , Sun Xiaodong 
Published: September 2022

Abstract
Background
Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-derived RPE) are a promising source for cell-replacement therapy to treat retinal degenerative diseases, but research on RPE cryopreservation is limited. This study aimed to determine the best phase for RPE cryopreservation to preserve the post-thaw function and uncover the mechanism underlying RPE freezing tolerance.

Methods
hESC-derived RPE cells were cryopreserved at various time points after seeding. After thawing, the survival and attachment rates, RPE marker gene expression, apical-basal polarity, PEDF secretion, transepithelial resistance, and phagocytotic ability of post-thaw RPE cells were evaluated. RNA sequencing was performed on RPE cells at three-time points, differentially expressed genes were identified, and gene ontology, Kyoto encyclopedia of genes and genomes, and protein–protein interaction analyses were used to investigate the key pathways or molecules associated with RPE cell freezing tolerance.

Results
RPE frozen at passage 2 day 5 (P2D5) had the highest cell viability and attachment after thawing. They also retained properly localized expression of RPE marker genes and biological functions such as PEDF secretion, high transepithelial resistance, and phagocytic ability. The RNA-sequencing analysis revealed that RPE cells at P2D5 expressed high levels of cell cycle/DNA replication and ECM binding associated genes, as well as THBS1, which may serve as a possible hub gene involved in freezing tolerance. We also confirmed that the RPE cells at P2D5 were in the exponential stage with active DNA replication.

Conclusions
We propose that freezing hESC-derived RPE cells during their exponential phase results in the best post-thawing outcome in terms of cell viability and preservation of RPE cell properties and functions. The high expression levels of the cell cycle and ECM binding associated genes, particularly THBS1, may contribute to better cell recovery at this stage.
Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells
Authors: Bi Youkun , Qiao Xinlong , Liu Qun , Song Shaole , Zhu Keqi , Qiu Xun , Zhang Xiang , jia Ce , Wang Huiwen , Yang Zhiguang , Zhang Ying , Ji Guangju 
Published: September 2022

Abstract
Background
Increasing studies have reported the therapeutic effect of mesenchymal stem cell (MSC)-derived exosomes by which protein and miRNA are clearly characterized. However, the proteomics and miRNA profiles of exosomes derived from human embryonic stem cells (hESCs) and human-induced pluripotent stem cells (hiPSCs) remain unclear.

Methods
In this study, we isolated exosomes from hESCs, hiPSCs, and human umbilical cord mesenchymal stem cells (hUC-MSCs) via classic ultracentrifugation and a 0.22-μm filter, followed by the conservative identification. Tandem mass tag labeling and label-free relative peptide quantification together defined their proteomics. High-throughput sequencing was performed to determine miRNA profiles. Then, we conducted a bioinformatics analysis to identify the dominant biological processes and pathways modulated by exosome cargos. Finally, the western blot and RT-qPCR were performed to detect the actual loads of proteins and miRNAs in three types of exosomes.

Results
Based on our study, the cargos from three types of exosomes contribute to sophisticated biological processes. In comparison, hESC exosomes (hESC-Exos) were superior in regulating development, metabolism, and anti-aging, and hiPSC exosomes (hiPSC-Exos) had similar biological functions as hESC-Exos, whereas hUC-MSCs exosomes (hUC-MSC-Exos) contributed more to immune regulation.

Conclusions
The data presented in our study help define the protein and miRNA landscapes of three exosomes, predict their biological functions via systematic and comprehensive network analysis at the system level, and reveal their respective potential applications in different fields so as to optimize exosome selection in preclinical and clinical trials.
Cancer cells as a new source of induced pluripotent stem cells
Authors: Shamsian Azam , Sahebnasagh Roxana , Norouzy Amir , Hussein Safin Hassan , Ghahremani Mohammad Hossein , Azizi Zahra 
Published: September 2022

AbstractOver the last 2 decades, induced pluripotent stem cells (iPSCs) have had various potential applications in various medical research areas, from personalized medicine to disease treatment. Different cellular resources are accessible for iPSC generation, such as keratinocytes, skin fibroblasts, and blood or urine cells. However, all these sources are somatic cells, and we must make several changes in a somatic cell’s transcriptome and chromatin state to become a pluripotent cell. It has recently been revealed that cancer cells can be a new source of iPSCs production. Cancer cells show similarities with iPSCs in self-renewal capacity, reprogramming potency, and signaling pathways. Although genetic abnormalities and potential tumor formation in cancer cells pose a severe risk, reprogrammed cancer-induced pluripotent stem cells (cancer-iPSCs) indicate that pluripotency can transiently overcome the cancer phenotype. This review discusses whether cancer cells can be a preferable source to generate iPSCs.
Relationship between mucosal TNF-α expression and Th1, Th17, Th22 and Treg responses in Helicobacter pylori infection
Authors: Rahimian Ghorbanali , Shahini Shams Abadi Milad , Mirzaei Yousef , Hussein Mer Ali , Ahmadi Reza , Azadegan-Dehkordi Fatemeh 
Published: September 2022

AbstractHelicobacter pylori (H. pylori)-induced gastric inflammation in the gastric mucosa and significantly increases the risk of developing gastritis and peptic ulcer disease (PUD). The objective of this research is to determine the role of tumor necrosis factor-α (TNF-α) expression in the gastric mucosa of patients with H. pylori-associated gastritis and PUD compared to uninfected patients, and we determined the relation between TNF-α expression and Th1/Th17/Th22, and Treg cells. Fifty-five patients with H. pylori-associated gastritis, 47 patients with H. pylori-associated PUD, and 48 uninfected patients were in this research. Antrum biopsy was used to detect H. pylori, virulence factors and histopathological assessments. Expression of TNF-α in the infected group was significantly higher than the uninfected group. Also, cagA/oipA-positive infected patients induce significantly more TNF-α expression than do cagA/oipA-negative infected patients. Expression of TNF-α was significantly increased in the PUD group than the gastritis group. Notably, TNF-α expression had a significant positive correlation with the frequency of Th1/Th17/Th22 lymphocytes in the PUD group. These findings indicate the importance of increasing TNF-α with Th1, Th17, Th22 responses increase as an important risk factor for PUD in context of H. pylori infection.
Superposition of rectangular power pulses and CP-OFDM signal for SWIPT
Authors: Kassab Hussein , Rottenberg François , Feuillen Thomas , Wiame Charles , Louveaux Jérôme 
Published: September 2022

AbstractSimultaneous wireless information and power transfer (SWIPT) has recently attracted researchers and may help to satisfy future technology demands. SWIPT allows wireless power transfer (WPT) and wireless information transfer (WIT) to coexist based on shared resources. Recent studies have shown that, due to the nonlinearity of the rectifiers, high-PAPR (peak to average power ratio) waveforms provide better performance in terms of energy harvesting, making the design of power signals essential. In addition, these power signals should consume the smallest amount of resources for the WIT. In this paper, a new waveform design is proposed where the information and power signals are superposed using the same frequency and time resources. The power signal is composed of a high peak modulated rectangular wave sent during the cyclic prefix of the orthogonal frequency-division multiplexing (CP-OFDM) system, which is discarded at the information receiver, such that it does not interfere with the OFDM data symbol. Although the pulse is restricted to be within the cyclic prefix, there might be a small amount of interference caused by channel dispersion. Simulations and measurements show that a good choice of signal parameters can minimize interference on the information symbols and simultaneously provide good performance in terms of energy harvesting.
Effects of psychological fatigue on college athletes’ error-related negativity based on artificial intelligence computing method
Authors: Li Jin , Wang Yanni , Li Sihua 
Published: September 2022

AbstractPsychological fatigue includes mental fatigue and burnout. In order to investigate the impact of psychological fatigue on athletes' response monitoring, event-related potentials technique is typically used, and the most critical indicator is error-related negativity. Two experiments were carried out to explore cause-effect relationships of psychological fatigue and response monitoring. The event-related potentials data processing was based on Artificial Intelligence computing methods, including wavelet transform, grayscale transformation and other algorithms. The first experiment was done to explain mental fatigue and response monitoring. 15 participants operated continuously 60 min Flanker task, and then operated 15 min task accompanied by light music. From the results of behavioral performances, the fatigue period compared with the fatigue-free period showed significant differences, including reaction time (p = 0.029) and correct rate (p = 0.046). From the results of error-related negativity, the amplitude of the fatigue-free period was bigger than that of the music adjustment period, the latter was bigger than that of the fatigue period (p < 0.001). The second experiment was conducted to explore burnout and response monitoring. Twenty-four participants were separated averagely into two groups. One group was burnout group, the other group was no burnout group. They both operated 15 min task. From the results of behavioral performances, no burnout group was better than burnout group. From the results of error-related negativity, no burnout group’s amplitude was bigger than that of burnout group, but the difference was marginally significant. The conclusions are as follows: Artificial intelligence technology is feasible for processing event-related potentials data. Psychological fatigue weakens athletes’ response monitoring ability, and the effect of mental fatigue is significant. In future researches, the following topics should be concerned, including the mediating or moderating effects of third variables, different ways of recovering from mental fatigue, computer data simulator and date accuracy, brain-computer interfaces and error-related negativity, etc.
A new existence result for some nonlocal problems involving Orlicz spaces and its applications
Authors: Qiu Xiaohui , Yan Baoqiang 
Published: September 2022

AbstractThis paper studies some quasilinear elliptic nonlocal equations involving Orlicz–Sobolev spaces. On the one hand, a new sub-supersolution theorem is proved via the pseudomonotone operator theory; on the other hand, using the obtained theorem, we present an existence result on the positive solutions of a singular elliptic nonlocal equation. Our work improves the results of some previous researches.
Hunting of hunted: an ensemble modeling approach to evaluate suitable habitats for caracals in India
Authors: Jangid Ashish Kumar , Singh Chandra Prakash , Parihar Jai Singh , Chauhan Jasbir Singh , Singh Rajnish Kumar , Verma Prakash Kumar , Singh Amritanshu , Sharma Shantanu , Kolipaka Shekhar 
Published: September 2022

AbstractBackgroundLarge-scale hunting and various anthropogenic pressures in the recent past have pushed the Asiatic caracal (Caracal caracal schmitzi), an elusive medium-sized and locally threatened felid species towards local extinction in India. Though widely distributed historically, it has been sparsely reported from several regions of central and northern states in India till twentieth century. Later, the species distribution became confined only to the states of Rajasthan, Gujarat and Madhya Pradesh, which have had reported sightings in the twenty-first century. In order to highlight the potentially suitable habitats for Asiatic caracals in India, we targeted forth-filtering of the spatial model ensemble by creating and utilizing the validated and spatially thinned species presence information (n = 69) and related ecological variables (aridity, NDVI, precipitation seasonality, temperature seasonality, terrain ruggedness), filtered with anthropological variable (nightlight).ResultsOut of eight spatial prediction models, the two most parsimonious models, Random Forest (AUC 0.91) and MaxEnt (AUC 0.89) were weighted and ensembled. The ensemble model indicated several clustered habitats, covering 1207.83 km2areas in Kachchh (Gujarat), Aravalli mountains (Rajasthan), Malwa plateau (Rajasthan and Madhya Pradesh), and Bundelkhand region (Madhya Pradesh) as potentially suitable habitats for caracals. Output probabilities of pixels were further regressed with converted vegetation height data within selected highly potential habitats, i.e., Ranthambore Kuno Landscape (RKL) (suitability ~ 0.44 + 0.03(vegetation height) **,R2= 0.27). The regression model inferred a significant positive relation between vegetation height and habitat suitability, hence the lowest ordinal class out of three classes of converted vegetation height was masked out from the RKL, which yielded in an area of 567 km2as potentially highly suitable habitats for caracals, which can be further proposed as survey areas and conservation priority areas for caracals.ConclusionThe study charts out the small pockets of landscape in and around dryland protected areas, suitable for caracal in the Indian context, which need attention for landscape conservation.
Development and validation of the parents’ cognitive perception inventory of disaster effects on children’s well-being (PCP-DCWB)
Authors: Atazadeh Najibeh , Mahmoodi Hassan , Sarbakhsh Parvin , Shaghaghi Abdolreza 
Published: September 2022

Abstract
Background
Parents’ cognition about the type and nature of consequences a disaster may pose on the children’s psychosocial health, could be a major protective factor against the long-term overwhelming complications. Given the lack of a reliable instrument to measure parents’ cognition about disasters’ effects on children’s well-being, this study was conducted to develop and validate the parents’ cognitive perception inventory of disaster effects on children’s well-being (PCP-DCWB).

Methods
In this cross-sectional study 300 parents of the survived primary school aged children from the Iran’s northwest earthquake on August 2012 were recruited in the city of Varzegan. Exploratory factor analysis (EFA) was applied to identify the subcomponents and Cronbach’s alpha and Guttmann Split-half coefficients were calculated to assess the internal consistency reliability of the scale.

Results
Structural indicators of the Kaiser–Meyer–Olkin measure (0.69) and Bartlett’s test of Sphericity (P < 0.001, df = 153, X2 = 618.35) verified interpretability of the EFA output. Applying principal component analysis and direct oblimin rotation in the EFA four latent factors were identified (i.e., perception about child overall mental health, coping with trauma’s long-term effects, children or parents’ continuing memory of past disaster and perception about behavioral and educational problems) which explained 49.32% of the total variance. The estimated Cronbach’s alpha and split-half reliability coefficients (0.71 and 0.52 respectively) supported good internal consistency of the instrument.

Conclusion
The study findings revealed sound psychometric attributes of the PCP-DCWB to be applied in assessment of parents’ cognition about psychological impacts of a traumatic event on the survived children. The instrument application can shed light on level of pre-disaster preparations in local, national and international scales and help effectiveness assessment of interventions that target maintenance of psycho-social well-being among disaster-affected survivors over time.
Augmented reality-aided unicompartmental knee arthroplasty
Authors: Tsukada Sachiyuki , Ogawa Hiroyuki , Kurosaka Kenji , Saito Masayoshi , Nishino Masahiro , Hirasawa Naoyuki 
Published: September 2022

Abstract
Purpose
To illustrate a surgical technique for augmented reality (AR)-assisted unicompartmental knee arthroplasty (UKA) and report preliminary data.

Methods
We developed an AR-based navigation system that enables the surgeon to see the tibial mechanical axis superimposed on the patient’s leg in addition to the tibial cutting angle. We measured the tibial resection angle in 11 UKAs using postoperative radiographs and calculated the absolute difference between preoperative target angle and postoperative measured angle. The target angle was determined for each patient: mean values were 0.7° ± 1.0° varus in coronal alignment and 5.3° ± 1.4° posterior slope in sagittal alignment.

Results
The angles measured on postoperative radiographs were 2.6° ± 1.2° varus in the coronal plane and 4.8° ± 2.5° posterior slope in the sagittal plane. The absolute differences between the target and measured angles were 1.9° ± 1.5° in coronal alignment and 2.6° ± 1.2° in sagittal alignment. No patients experienced complications, including surgical site infection and periprosthetic fracture.

Conclusion
The AR-based portable navigation system may provide passable accuracy in terms of proximal tibial resection during UKA.

Level of Evidence
IV
The use of advanced imaging in guiding the further investigation and treatment of primary prostate cancer
Authors: Duan Heying , Iagaru Andrei 
Published: September 2022

AbstractIn the era of precision medicine, oncological imaging techniques are advancing at a rapid pace, particularly molecular imaging with promising new targets for prostate cancer (PC) such as gastrin releasing peptide receptors (GRPR) along the established and indispensable prostate specific membrane antigen (PSMA). As PC is characterized by heterogenous tumor biology ranging from indolent to aggressive disease, distinguishing clinically significant tumors from indolent disease is critical. Multiparametric MRI- and PET-targeted prostate biopsies mitigate the shortcomings and risks of standard systematic template biopsy by identifying more significant cancers.Focal treatment for localized disease is a minimally invasive approach that targets the index tumor – the lesion of the highest grade – while sparing the surrounding healthy tissue. Real-time MRI-guidance and thermal control with MR-thermometry, improves treatment accuracy and results in lower rates of functional side effects. PET imaging could be an useful tool to assess response to treatment compared to invasive prostate biopsies.In this comprehensive review, we focus on the image-guided detection and treatment of localized primary prostate cancer, its current status and future perspectives.
Intravoxel incoherent motion imaging combined with diffusion kurtosis imaging to assess the response to radiotherapy in a rabbit VX2 malignant bone tumor model
Authors: Guo Jia , Sun Weikai , Dong Cheng , Wu Zengjie , Li Xiaoli , Zhou Ruizhi , Xu Wenjian 
Published: September 2022

Abstract
Purpose
To combine intravoxel incoherent motion (IVIM) imaging and diffusion kurtosis imaging (DKI) parameters for the evaluation of radiotherapy response in rabbit VX2 malignant bone tumor model.

Material and methods
Forty-seven rabbits with bone tumor were prospectively enrolled and divided into pre-treatment, considerable effect and slight effect group. Treatment response was evaluated using IVIM-DKI. IVIM-based parameters (tissue diffusion [Dt], pseudo-diffusion [Dp], perfusion fraction [fp]), and DKI-based parameters (mean diffusion coefficient [MD] and mean kurtosis [MK]) were calculated for each animal. Corresponding changes in MRI parameters before and after radiotherapy in each group were studied with one-way ANOVA. Correlations of diffusion parameters of IVIM and DKI model were computed using Pearson’s correlation test. A diagnostic model combining different diffusion parameters was established using binary logistic regression, and its ROC curve was used to evaluate its diagnostic performance for determining considerable and slight effect to malignant bone tumor.

Results
After radiotherapy, Dt and MD increased, whereas fp and MK decreased (p <  0.05). The differences in Dt, fp, MD, and MK between considerable effect and slight effect groups were statistically significant (p <  0.05). A combination of Dt, fp, and MK had the best diagnostic performance for differentiating considerable effect from slight effect (AUC = 0.913, p <  0.001).

Conclusions
A combination of IVIM- and DKI-based parameters allowed the non-invasive assessment of cellular, vascular, and microstructural changes in malignant bone tumors after radiotherapy, and holds great potential for monitoring the efficacy of tumor radiotherapy.