bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomes of Vibrio metoecus co-isolated with Vibrio cholerae extend our understanding of differences between these closely related species
Authors: Orata Fabini D. , Hussain Nora A. S. , Liang Kevin Y. H. , Hu Dalong , Boucher Yann F. 
Published: November 2022

Abstract
Background
Vibrio cholerae, the causative agent of cholera, is a well-studied species, whereas Vibrio metoecus is a recently described close relative that is also associated with human infections. The availability of V. metoecus genomes provides further insight into its genetic differences from V. cholerae. Additionally, both species have been co-isolated from a cholera-free brackish coastal pond and have been suggested to interact with each other by horizontal gene transfer (HGT).

Results
The genomes of 17 strains from each species were sequenced. All strains share a large core genome (2675 gene families) and very few genes are unique to each species (< 3% of the pan-genome of both species). This led to the identification of potential molecular markers—for nitrite reduction, as well as peptidase and rhodanese activities—to further distinguish V. metoecus from V. cholerae. Interspecies HGT events were inferred in 21% of the core genes and 45% of the accessory genes. A directional bias in gene transfer events was found in the core genome, where V. metoecus was a recipient of three times (75%) more genes from V. cholerae than it was a donor (25%).

Conclusion
V. metoecus was misclassified as an atypical variant of V. cholerae due to their resemblance in a majority of biochemical characteristics. More distinguishing phenotypic assays can be developed based on the discovery of potential gene markers to avoid any future misclassifications. Furthermore, differences in relative abundance or seasonality were observed between the species and could contribute to the bias in directionality of HGT.
Fluoxetine for the treatment of onychotillomania associated with obsessive–compulsive disorder: a case report
Authors: Aljhani Sumayah 
Published: November 2022

Abstract
Background
Obsessive–compulsive disorder is a condition in which patients experience an obsession and/or a compulsion. It has a high impact on the quality of life, and is associated with an increased prevalence of psychiatric comorbidities in patients. Onychotillomania is an underestimated psychodermatosis caused by repeated self-inflicted damage to the nail unit. In patients, it is characterized by an obsessive or irrepressible impulse to repeatedly damage their own nails, resulting in their destruction. It is a chronic condition that is difficult to manage, largely because of its psychocutaneous character, as well as its high tendency to interact with underlying neuropsychiatric diseases or other behavioral disorders. Only a few studies have reported an association between obsessive–compulsive disorder and onychotillomania, which typically presents with therapeutic challenges. Cognitive behavioral therapy, physical-barrier approaches, and pharmaceutical treatments have been reported to be beneficial in the management of onychotillomania; however, no major clinical studies have investigated the effectiveness of these therapies. Onychotillomania remains a clinical and therapeutic issue owing to the lack of evidence-based treatment techniques.

Case presentation
We report a case of an 18-year-old, middle-eastern female patient who developed onychotillomania when she was being treated with paroxetine for obsessive–compulsive disorder and was showing partial improvement. The patient developed side effects from paroxetine, and was switched to fluoxetine. Thereafter, improvement in her obsessive–compulsive disorder was observed, which relapsed when treatment was discontinued. However, the onychotillomania symptoms did not reemerge.

Conclusion
Onychotillomania typically presents both diagnostic and therapeutic challenges. Fluoxetine plays an important role in the treatment of onychotillomania and other psychiatric disorders. However, large-scale studies should be conducted before these outcomes can be generalized.
The pattern of dyslipidemia among Somali type 2 diabetic patients: a cross-sectional study
Authors: Alıcı Gökhan , Genç Ömer 
Published: November 2022

Abstract
Background
Diabetes mellitus (DM) is a major public health concern. This study aims to determine frequency, pattern, and potential determinants of dyslipidemia among adults with type 2 DM (T2DM) at Somalia’s only diabetes outpatient clinic.

Methods
Five hundred twenty-nine consecutive patients with T2DM who applied to our outpatient clinic between January 2020 and June 2020 were included in this cross-sectional hospital-based study. Demographic characteristics of participants, including lipid panel, were extracted from the registry system. Correlation analysis was performed between lipid profile and related parameters. Multivariate binary logistic regression models were used to identify independent determinants of dyslipidemia for further analysis.

Results
The overall population’s mean age was 51.9 ± 12.2 years, with 177 (33.5%) males. Total and atherogenic dyslipidemias were found in 92.8% and 24.8%, respectively. The most common isolated pattern of dyslipidemia was high non-high-density lipoprotein cholesterol (non-HDL-C) (82.8%), followed by high low-density lipoprotein cholesterol (LDL-C) (72.6%), high total cholesterol (TC) (54.3%), and low HDL-C (48.3%). Females were found to have a higher prevalence of high TC (63.4% vs. 54.2%, p = 0.043) and lower HDL-C (57.4% vs. 46.3%, p = 0.016). High LDL-C with low HDL-C was the most common pattern among combined type dyslipidemias (18.1%), followed by high LDL-C with high triglyceride (TG) (17.8%), as well as low TG with low HDL-C (3.6%). Females had a higher proportion of high LDL-C with low HDL-C than males (20.3% vs. 13.6%, p = 0.036). Age, gender, body mass index, central obesity, spot urinary proteinuria, fasting blood glucose, poor glycemic control, creatinine, and Hs-CRP were all associated with different dyslipidemia patterns in multivariate logistic regression analyses.

Conclusions
We found that the prevalence of dyslipidemia, especially atherogenic patterns, was extremely high among Somali T2DM patients. An enhanced health policy should, therefore, be established to detect, treat and prevent dyslipidemia.
Hypertonic saline for traumatic brain injury: a systematic review and meta-analysis
Authors: Gharizadeh Nafiseh , Ghojazadeh Morteza , Naseri Amirreza , Dolati Sanam , Tarighat Faezeh , Soleimanpour Hassan 
Published: November 2022

AbstractBackgroundTraumatic brain injury (TBI) causes mortality and long-term disability among young adults and imposes a notable cost on the healthcare system. In addition to the first physical hit, secondary injury, which is associated with increased intracranial pressure (ICP), is defined as biochemical, cellular, and physiological changes after the physical injury. Mannitol and Hypertonic saline (HTS) are the treatment bases for elevated ICP in TBI. This systematic review and meta-analysis evaluates the effectiveness of HTS in the management of patients with TBI.MethodsThis study was conducted following the Joanna Briggs Institute (JBI) methods and PRISMA statement. A systematic search was performed through six databases in February 2022, to find studies that evaluated the effects of HTS, on increased ICP. Meta-analysis was performed using comprehensive meta-analysis (CMA).ResultsOut of 1321 results, 8 studies were included in the systematic review, and 3 of them were included in the quantitative synthesis. The results of the meta-analysis reached a 35.9% (95% CI 15.0–56.9) reduction in ICP in TBI patients receiving HTS, with no significant risk of publication bias (t-value = 0.38, df = 2,p-value = 0.73). The most common source of bias in our included studies was the transparency of blinding methods for both patients and outcome assessors.ConclusionHTS can significantly reduce the ICP, which may prevent secondary injury. Also, based on the available evidence, HTS has relatively similar efficacy to Mannitol, which is considered the gold standard therapy for TBI, in boosting patients' neurological condition and reducing mortality rates.
Synthesis of eco-friendly ZnO-based heterophotocatalysts with enhanced properties under visible light in the degradation of organic pollutants
Authors: Batchamen Mougnol Jean Bedel , Waanders Frans , Ntwampe Seteno Karabo Obed , Fosso-Kankeu Elvis , Al Alili Ali Rashed 
Published: November 2022

AbstractHeterogeneous photocatalysts have been widely used for the removal of various organic pollutants from wastewater. The main challenge so far resides in the sustainability of the process, with regard to the synthesis and the application under visible light. In this study the precipitated materials from the Moringa oleifera seed (MO), groundnut shells (GS) and apatite (A) agrowastes were functionalized with zinc oxide (ZnO) and silver (Ag) solution, to produce a novel bioheterophotocatalysts. Various analytical techniques such as scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), photoluminescence (PL) and X-ray diffraction (XRD) were used for the characterization of the novel photocatalysts. It was proven that agrowastes can also enhance the photocatalytic activity of a ZnO-based photocatalyst as pure metals. The combination of MO/GS/A/ZnO/Ag in a 1:1:1 ratio resulted in a lower band gap of 1.59 eV, as compared to the band gap of 2.96 eV for ZnO/Ag. These photocatalysts' efficiency was also tested on the photodegradation of polycyclic aromatic hydrocarbon (PAHs) derived from coal leaching in various water sources such as acidic mine drainage, alkaline mine drainage and sewage wastewater. From MO/GS/A/ZnO/Ag, the removal efficiency was found to be 69.59%, 61.07% and 61.68%, compared to 52.62%, 37.96 and 44.30% using ZnO/Ag in acidic mine drainage, alkaline mine drainage and sewage wastewater for 60 min under solar irradiation.
Adefovir dipivoxil inhibits APL progression through degradation of the oncoprotein PML-RARA
Authors: Gong Xubo , Kong Piaoping , Yu Teng , Xiao Xibin , Wang Lin , Sang Yiwen , Li Xiang , Zhang Bin , Tao Zhihua , Liu Weiwei 
Published: November 2022

AbstractAcute promyelocytic leukemia (APL) is highly aggressive and is frequently associated with disseminated intravascular coagulation and high early death rates. Although all-trans retinoic acid (RA) induces complete remission in a high proportion of patients with APL, there are limited treatments for APL patients with RA resistance. Here we report an atypical APL patient, with an APL-like disease that developed very slowly without anti-leukemia therapy for nearly 2 years. During that time, the patient only intermittently received anti-HBV drugs, i.e., the combination of adefovir dipivoxil (ADV) and entecavir (ETV), leading us to hypothesize that ADV and/or ETV could inhibit APL progression. Our results showed that anti-HBV drugs ADV and ETV both exhibited significantly inhibitory effects on APL cells, and ADV indicated a much greater cytotoxic effect than ETV on APL cells. We further found that ADV significantly promoted APL cell differentiation and apoptosis, thereby restraining the progression of APL. Most importantly, our study uncovered a novel mechanism of ADV prohibiting APL progression, which was mediated, at least in part, by inhibition of TRIB3 and degradation of the oncoprotein PML-RARA, therefore leading to APL cell differentiation and apoptosis. Taken together, our study demonstrated that ADV, an anti-HBV drug, had significantly inhibitory effects on APL, and provided a novel therapeutic strategy for APL patients with RA resistance.
Chronic kidney disease prediction using machine learning techniques
Authors: Debal Dibaba Adeba , Sitote Tilahun Melak 
Published: November 2022

AbstractGoal three of the UN’s Sustainable Development Goal is good health and well-being where it clearly emphasized that non-communicable diseases is emerging challenge. One of the objectives is to reduce premature mortality from non-communicable disease by third in 2030. Chronic kidney disease (CKD) is among the significant contributor to morbidity and mortality from non-communicable diseases that can affected 10–15% of the global population. Early and accurate detection of the stages of CKD is believed to be vital to minimize impacts of patient’s health complications such as hypertension, anemia (low blood count), mineral bone disorder, poor nutritional health, acid base abnormalities, and neurological complications with timely intervention through appropriate medications. Various researches have been carried out using machine learning techniques on the detection of CKD at the premature stage. Their focus was not mainly on the specific stages prediction. In this study, both binary and multi classification for stage prediction have been carried out. The prediction models used include Random Forest (RF), Support Vector Machine (SVM) and Decision Tree (DT). Analysis of variance and recursive feature elimination using cross validation have been applied for feature selection. Evaluation of the models was done using tenfold cross-validation. The results from the experiments indicated that RF based on recursive feature elimination with cross validation has better performance than SVM and DT.
Long-term follow-up of vagus nerve stimulation in drug-resistant KCNT1-related epilepsy: a case presentation
Authors: Wang Meng , Geng Guifu , Meng Yao , Zhang Hongwei , Gao Zaifen , Shi Jianguo 
Published: November 2022

Abstract
Background
The KCNT1 gene encodes a Na+-activated K+ channel. Gain-of-function mutations of KCNT1 lead to autosomal dominant sleep-related hypermotor epilepsy, early-onset epileptic encephalopathy, focal epilepsy and other epileptic encephalopathies. In this paper, we report a boy carrying a KCNT1 gene mutation, who presented with drug-resistant focal-onset seizures. He had decreased seizure frequency and improvement of background changes in electroencephalography (EEG) after vagus nerve stimulation (VNS).

Case presentation
The case was a nonverbal 9-year-old male who presented with drug-resistant focal-onset seizures since age 3 and had underwent VNS therapy for 2 years. He had hypermotor symptoms, automatism and bilateral asymmetric tonic seizures with cognitive decline and aphasis from age 3. The patient had a variety of seizure types that only occurred at night. The most common seizure type was automatisms, and ictal video EEG showed high-amplitude delta waves, followed by a fast rhythmic sharp activity in the mesial frontal and bitemporal regions. The patient was diagnosed with KCNT1-related epilepsy, epileptic encephalopathy and cognitive disorder. He was refractory to multiple anti-seizure medicines (ASM) and ketogenic diet. After VNS treatment at age 7, the frequency of seizures was reduced significantly and EEG was improved in background slowing.

Conclusions
Children with KCNT1-related epilepsy usually have early onset of disease, are nonverbal, and are refractory to ASM. This boy with drug-resistant KCNT1-related epilepsy showed significantly reduced seizure frequency after VNS. This report may provide reference for management of cases of KCNT1-related epilepsy.
Modulation of gut microbiota, blood metabolites, and disease resistance by dietary β-glucan in rainbow trout (Oncorhynchus mykiss)
Authors: Menanteau-Ledouble Simon , Skov Jakob , Lukassen Mie Bech , Rolle-Kampczyk Ulrike , Haange Sven-Bastiaan , Dalsgaard Inger , von Bergen Martin , Nielsen Jeppe Lund 
Published: November 2022

Abstract
Background
Prebiotics are known to have a positive impact on fish health and growth rate, and β-glucans are among the most used prebiotics on the market. In this study, rainbow trout (Oncorhynchus mykiss) were treated with a β-1,3;1,6-glucan dietary supplement (at a dose of 0 g, 1 g, 10 g, and 50 g β-glucan per kg of feed). After 6 weeks, the effect of the β-glucan was evaluated by determining the changes in the microbiota and the blood serum metabolites in the fish. The impact of β-glucan on the immune system was evaluated through a challenge experiment with the bacterial fish pathogen Yersinia ruckeri.

Results
The microbiota showed a significant change in terms of composition following β-glucan treatment, notably an increase in the relative abundance of members of the genus Aurantimicrobium, associated with a decreased abundance of the genera Carnobacterium and Deefgea. Furthermore, analysis of more than 200 metabolites revealed that the relative levels of 53 metabolites, in particular compounds related to phosphatidylcholines, were up- or downregulated in response to the dietary supplementation, this included the amino acid alanine that was significantly upregulated in the fish that had received the highest dose of β-glucan. Meanwhile, no strong effect could be detected on the resistance of the fish to the bacterial infection.

Conclusions
The present study illustrates the ability of β-glucans to modify the gut microbiota of fish, resulting in alteration of the metabolome and affecting fish health through the lipidome of rainbow trout.
Maternal knowledge, coping strategies, and metabolic control of children with type 1 diabetes
Authors: Khallaf Nabila Mohammed Raouf , Manzour Ayat Farouk , Mostafa Aya , Salam Meky Fatma Abdel 
Published: November 2022

Abstract
Background
Trends of type 1 diabetes have increased among Egyptian children in the past two decades. Managing a child with type 1 diabetes is a great challenge for parents especially when the child is not developmentally able to manage the disease independently. This study aimed to determine level of diabetes-specific knowledge among diabetic children’s mothers, measure maternal diabetes-related stress, identify diabetes-related maternal coping strategies, and to examine the effect of maternal diabetes-specific knowledge, maternal diabetes-related stress, and their adopted coping strategy on glycemic control of their children. A cross-sectional study was conducted in outpatient clinic for diabetic children at a university hospital in Cairo, Egypt. One hundred and four mothers of diabetic children were recruited. Diabetes-specific knowledge was measured by Modified Diabetes Knowledge Questionnaire. Diabetes-related stress and coping strategies were measured by the Ways of Coping Questionnaire. Glycosylated hemoglobin level was obtained from medical records.

Results
61.5% of mothers had good diabetes-specific knowledge; however, 45.2% had high levels of diabetes-related stress. The most common adopted maternal coping strategy was acceptance of child’s illness (71.6%). Glycosylated hemoglobin level in diabetic children was negatively correlated with positive and wishful thinking (p = 0.042 and p = 0.010 respectively) and acceptance of illness (p = 0.002), while positively correlated with cognitive restructuring (p = 0.007).

Conclusions
Health education to train mothers to adopt positive coping strategies might maintain a better glycemic control in diabetic children in similar settings.
Effect of shot peening on the critical buckling load of stainless steel 304 columns immersed in sea water
Authors: Bader Amjed M. , Alazawi Dhia A. , Al-Alkawi Hussain J. M. , Faris Saad T. 
Published: November 2022

Abstract
A machine part subjected to an axial compressive load is called strut. But, a vertical strut is known as a column. The machine members that must be investigated for the column action are connecting rods, piston rods, screw jack, etc. When a column is subjected to a compressive load and this load is gradually increased, a stage will be reached when the column will be subjected to ultimate load. Beyond this, the column will fail by crushing, and the load will be known as crushing load. When the column is short or intermediate, sometimes this column fails by bending, i.e. buckling. When the column is long, the value of buckling load is low for long columns and is relatively high for intermediate columns. The present investigation focuses on the testing and evaluation of the mechanical end buckling columns (samples) using 304 stainless steel under dry, corrosion, and combined dry with shot peening (SP) process. The buckling behavior of the axial compressive load has been studied experimentally and theoretically using Euler and Rankin theories. The results obtained from the above study manifested that the column, whose slenderness ratio (SR) is more than 120, is denoted as the long column, and the Euler theory can be successfully used. But, when the SR is less than 120, the column is known as an intermediate one. The mechanical and buckling properties exhibited a reduction due to the corrosion media and a reasonable improvement when using SP.
Forecasting post-earthquake rockfall activity
Authors: Olsen Michael J. , Massey Chris , Leshchinsky Ben , Wartman Joseph , Senogles Andrew 
Published: November 2022

Abstract
Important infrastructure such as highways or railways traverse unstable terrain in many mountainous and scenic parts of the world. Rockfalls and landslides result in frequent maintenance needs, system unreliability due to frequent closures and restrictions, and safety hazards. Seismic activity significantly amplifies these negative economic and community impacts by generating large rockfalls and landslides as well as weakening the terrain. This paper interrogates a rich database of repeat terrestrial lidar scans collected during the Canterbury New Zealand Earthquake Sequence to document geomorphic processes as well as quantify rockfall activity rates through time. Changes in the activity rate (spatial distribution) and failure depths (size) were observed based on the Rockfall Activity Index (RAI) morphological classification. Forecasting models can be developed from these relationships that can be utilized by transportation agencies to estimate increased maintenance needs for debris removal to minimize road closures from rockfalls after seismic events.
Ultrafiltration and reuse opportunities of sectorial effluents from a kraft pulp mill in Brazil
Authors: Rosado Corrêa Filho José Roberto , Silva Cláudio Mudadu , Diniz Caldeira Daiane Cristina , Coelho Dalvi Leandro , Araújo Nascimento Deyvisson , Silva Leite Rodrigo , Gomes Veloso Marcus Vinicius , Lemos Felício Agostinho Luewton , Timbalari Gleb , Cappon Hans , Guimarães Viola Zenilde das Graças , Andrada Maria Marina 
Published: November 2022

Abstract
The pulp industry is a high natural resources consumer and has a large polluting potential. The pulping sector with the most effluent generation is the bleaching plant. Based on the need of water consumption reduction, this research aimed to evaluate the treatment of the alkaline effluent from an EP-stage bleaching plant and the white water from drying machine using ultrafiltration membranes in order to enable the reuse of the permeate in the process. An ultrafiltration pilot plant was used. Laboratory analyses were performed for the feed, permeate and retentate. Flux and transmembrane pressure (TMP) data were obtained from the pilot plant database. There were an average color and COD removals of 38 % and 39 %, respectively, for the EP-effluent and 58 % and 67 %, respectively for the white water; and a high turbidity and TSS removal (above 98 %) for both effluents. Substantial metals removal such as sodium, potassium, calcium, iron, magnesium and manganese was achieved. Some possibilities to reuse were considered: return to the water treatment plant, cooling of the digester bottom, equipment cooling, reuse in evaporation surface condensers. The use of ultrafiltration for the treatment of the alkaline effluent from the bleaching EP-stage and white water can be an interesting option.