bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple Genetic Alterations Cause Frequent and Heterogeneous Human Histocompatibility Leukocyte Antigen Class I Loss in Cervical Cancer
Authors: Koopman Louise A. , Corver Willem E. , van der Slik Arno R. , Giphart Marius J. , Fleuren Gert Jan 
Published: July 2002

The nature and frequency of human histocompatibility leukocyte antigen (HLA) class I loss mechanisms in primary cancers are largely unknown. We used flow cytometry and molecular analyses to concurrently assess allele-specific HLA phenotypes and genotypes in subpopulations from 30 freshly isolated cervical tumor cell suspensions.
Tumor-associated HLA class I alterations were present in 90% of the lesions tested, comprising four altered pheno/genotype categories: (a) HLA-A or -B allelic loss (17%), mostly associated with gene mutations; (b) HLA haplotype loss, associated with loss of heterozygosity at 6p (50%). This category included cases with additional loss of a (third) HLA-A or -B allele due to mutation, as well as one case with an HLA class I–negative tumor cell subpopulation, caused by a β2-microglobulin gene mutation; (c) Total HLA class I antigen loss and retention of heterozygosity (ROH) at 6p (10%); and (d) B locus or HLA-A/B downregulation associated with ROH and/or allelic imbalance at 6p (10%). Normal HLA phenotypes and ROH at 6p were observed in 10% of the cases. One case could not be classified (3%).
Altered HLA class I antigen expression occurs in most cervical cancers, is diverse, and is mainly caused by genetic changes. Combined with widespread tumor heterogeneity, these changes have profound implications for natural immunity and T cell–based immunotherapy in cervical cancer.
Fully distributed containment of multiple‐input‐multiple‐output linear multi‐agent systems with multiple nonautonomous leaders
Authors: Tian Huanhuan , Wang Peijun , Huang Tingwen 
Published: August 2022

SummaryIn this article, we study the fully distributed containment problem for multiple‐input‐multiple‐output linear multi‐agent systems (MASs) with multiple nonautonomous leaders (i.e., leaders with nonzero inputs). First, based on an unknown input observer (UIO) with full order and by using unit vector method, we design an adaptive discontinuous controller, where the adaptive law is used to adjust network coupling strengths. And by using Barbǎlat lemma, we show that fully distributed containment can be achieved if the graph is connected and the subgraph among multiple followers is detail balanced. Second, we study fully distributed containment for MASs under an adaptive discontinuous controller based on a UIO with reduced order. Moreover, we study the case with directed switching topology. Finally, we perform simulations on a team of aircrafts to validate the theoretical results.
Construction of Long‐Wavelength Emissive Organic Nanosonosensitizer Targeting Mitochondria for Precise and Efficient In Vivo Sonotherapy
Authors: Zhang Xiangqian , Li Chonglu , Zhang Yi , Guan Xiaofang , Mei Longcan , Feng Huili , Li Jie , Tu Le , Feng Guoqiang , Deng Ganzhen , Sun Yao 
Published: July 2022

AbstractAs a modern biomedical therapeutic modality, sonodynamic therapy (SDT) presents unique advantages, including superior tissue penetration capability, temporal–spatial controllability, and negligible side effects. However, the bottlenecks of most organic sonosensitizers are their short emission wavelengths, strong phototoxicity, and unsatisfactory SDT effect, which undermines the precise fluorescence imaging‐guided SDT in vivo. Here, a long‐wavelength emissive and mitochondria‐targeted organic nanosonosensitizer, named CCNU980 nanoparticles (NPs), is rationally designed, which possesses deep‐tissue optical penetration (up to 6 mm), depth‐activated ROS production (up to 8 cm), high photostability, and low phototoxicity. In vitro studies verify CCNU980 NPs selectively enriches in cancer cells with the ability to target the mitochondria and induce mitochondria‐mediated apoptosis using abundant 1O2 under US irradiation. Notably, CCNU980 NPs enables precise in vivo NIR‐II fluorescence imaging‐guided SDT, accompanied by the suppression of the bilateral 4T1 tumor growth with minimal side effects. The current work can inspire a general strategy for the design of organic nanosonosensitizers with long‐wavelength emission and new thoughts for precision medicine.
Building a Self‐Adaptive Protective Layer on Ni‐Rich Layered Cathodes to Enhance the Cycle Stability of Lithium‐Ion Batteries
Authors: Yang Hua , Gao Rui‐Min , Zhang Xu‐Dong , Liang Jia‐Yan , Meng Xin‐Hai , Lu Zhuo‐Ya , Cao Fei‐Fei , Ye Huan 
Published: August 2022

AbstractLayered Ni‐rich lithium transition metal oxides are promising battery cathodes due to their high specific capacity, but their poor cycling stability due to intergranular cracks in secondary particles restricts their practical applications. Surface engineering is an effective strategy for improving a cathode's cycling stability, but most reported surface coatings cannot adapt to the dynamic volume changes of cathodes. Herein, a self‐adaptive polymer (polyrotaxane‐co‐poly(acrylic acid)) interfacial layer is built on LiNi0.6Co0.2Mn0.2O2. The polymer layer with a slide‐ring structure exhibits high toughness and can withstand the stress caused by particle volume changes, which can prevent the cracking of particles. In addition, the slide‐ring polymer acts as a physicochemical barrier that suppresses surface side reactions and alleviates the dissolution of transition metallic ions, which ensures stable cycling performance. Thus, the as‐prepared cathode shows significantly improved long‐term cycling stability in situations in which cracks may easily occur, especially under high‐rate, high‐voltage, and high‐temperature conditions.
Polishing the Lead‐Poor Surface for Efficient Inverted CsPbI3 Perovskite Solar Cells
Authors: Fu Sheng , Le Jiabo , Guo Xueming , Sun Nannan , Zhang Wenxiao , Song Weijie , Fang Junfeng 
Published: August 2022

AbstractTriiodide cesium lead perovskite (CsPbI3) has promising prospects in the development of efficient and stable photovoltaics in both single‐junction and tandem structures. However, achieving inverted devices that provide good stability and are compatible to tandem devices remains a challenge, and the deep insights are still not understood. This study finds that the surface components of CsPbI3 are intrinsically lead‐poor and the relevant traps are of p‐type with localized states. These deep‐energy‐level p traps induce inferior transfer or electrons and serious nonradiative recombination at the CsPbI3/PCBM interface, leading to the considerable open‐circuit voltage (Voc) loss and reduction of fill factor (FF). Compared to molecular passivation, polishing treatment with 1,4‐butanediamine can eliminate the nonstoichiometric components and root these intrinsically lead‐poor traps for superior electron transfer. The polishing treatment significantly improves the FF and Voc of the inverted CsPbI3 photovoltaics, creating an efficiency promotion from 12.64% to 19.84%. Moreover, 95% of the initial efficiency of the optimized devices is maintained after the output operation for 1000 h.
Treatment strategies for unruptured pancreaticoduodenal artery aneurysms associated with celiac artery occlusion
Authors: Kubota Koji , Shimizu Akira , Notake Tsuyoshi , Wada Yuko , Soejima Yuji 
Published: August 2022

AbstractCeliac artery (CA) occlusion or stenosis is identified in up to almost half of all patients undergoing abdominal angiography, and the resulting increased collateral blood flow from the superior mesenteric artery to the pancreaticoduodenal artery (PDA) may cause PDA aneurysms (PDAAs). PDAAs are rare but could be fatal if they rupture. However, treatment of the PDAA could block this important collateral blood flow pathway, leading to ischemic organ damage. Treatment of such aneurysms is therefore difficult, especially in patients with multiple PDAAs. Successful treatment of PDAAs requires establishing blood flow in the CA region and selecting which aneurysm(s) to treat. We present four patients who underwent surgery for unruptured PDAAs caused by CA obstruction. Blood flow in the CA region was established by bypassing the splenic artery and by anastomosing it either directly to the left renal artery (n = 1) or to the abdominal aorta using a graft (saphenous vein: n = 1; artificial vessel: n = 2). Three patients had multiple PDAAs: all PDAAs were treated in one patient with PDAAs of similar size and shape, but only the largest PDAA with the highest risk of rupture was treated in the other two patients to simplify the procedure. The median observation period was 19.5 months (range: 11‐28 months), and all patients were alive without recurrence at the time of writing. Surgical treatment including splenic artery bypass may thus be a viable option for treating patients with unruptured PDAAs.
Wall‐resolved large eddy simulation of turbulent flows in helically ribbed steam cracker reactors
Authors: Wang Yannan , Dedeyne Jens N. , Vandewalle Laurien A. , Van Geem Kevin M. 
Published: August 2022

AbstractHelically ribbed coils are commonly applied in steam cracking furnaces. To fully understand the impact of these ribbed wall modifications on the local heat transfer and associated pressure drop throughout the reactor, detailed experimental, and numerical studies have been performed. Experimental data based on stereo‐particle image velocimetry (S‐PIV) and liquid crystal thermography have been used to validate the numerical results from wall‐resolved large eddy simulations using OpenFOAM. The validation shows an excellent agreement in terms of mean and fluctuating velocities, pressure drop, and heat transfer behavior in a discontinuously ribbed tube. Compared with the pressure drop in a continuously ribbed tube, an approximately 40% lower pressure drop is obtained with a discontinuously ribbed tube, at the cost of a slightly decreased heat transfer enhancement. This makes the discontinuously ribbed tube design particularly interesting for steam cracking applications. The results also show that the nonuniform heat transfer at the wall is inherently linked to the flow reattachment and recirculation zones caused by the rib. Finally, the validated numerical model was used to study comparable designs and propose novel helical rib designs. Based on the results of the study, enlarging the trailing edge of the conventional ribbed geometry will improve the thermal enhancement performance, and is therefore found most promising for steam cracking reactor design.
Case report of mild TCIRG1‐associated autosomal recessive osteopetrosis in Vietnam
Authors: Luong Long Hoang , Nguyen Hieu Dinh , Trung Tuyen Nguyen , Minh Tam Mai Thi , Khanh Trinh Le , Son Tung Pham , Tran Tien Dang , Nguyen Tran Thuy 
Published: August 2022

AbstractAutosomal recessive osteopetrosis (ARO) is a group of disease characterized by osteoclast dysfunction inhibiting bone resorption and bone turnover, with TCIRG1‐associated ARO being more common leading to autosomal recessive infantile malignant osteopetrosis (OPTB1, MIM entry number # 259700). While most patients with TCIRG1‐associated osteopetrosis present a malignant clinical course and shortened lifespan, a few cases of non‐malignant TCIRG1‐associated osteopetrosis have been reported. 24‐year‐old female patient came to us with limp gait, hip pain in both sides, and severe stiffness. She had suffered many fractures, bilateral hip osteoarthritis, right leg was 2 cm shorter compared with left leg. Whole Exome Sequencing was conducted, the result and subsequent Sanger's sequencing shown the patient had a compound heterozygous genotype at TCIRG1 (c.1194dup, p.Gly399ArgTer and c.334G>A, p.Gly112Arg), these two variants found were not previously reported. Sanger's sequencing revealed two other siblings whom suffer the same disorder had similar genotype to the proband; the parents were found to be heterozygous. This is the first case of TCIRG1‐associated osteopetrosis reported in Vietnam and one of the few cases of nonmalignant TCIRG1‐associated osteopetrosis, in which detailed clinical and genetic work‐up were performed.
Putting participants and study partners FIRST when clinical trials end early
Authors: Largent Emily A. , Walter Sarah , Childs Nancy , Dacks Penny A. , Dodge Shana , Florian Hana , Jackson Jonathan , Llibre Guerra Jorge J. , Iturriaga Erin , Miller David S. , Moreno Monica , Nosheny Rachel L. , Obisesan Thomas O. , Portacolone Elena , Siddiqi Bernadette , Silverberg Nina , Warren Rueben C. , Welsh‐Bohmer Kathleen A. , Edelmayer Rebecca M. ,   the Participant FIRST Work Group
Published: August 2022

AbstractBetween 2018 and 2019, multiple clinical trials ended earlier than planned, resulting in calls to improve communication with and support for participants and their study partners (“dyads”). The multidisciplinary Participant Follow‐Up Improvement in Research Studies and Trials (Participant FIRST) Work Group met throughout 2021. Its goals were to identify best practices for communicating with and supporting dyads affected by early trial stoppage. The Participant FIRST Work Group identified 17 key recommendations spanning the pre‐trial, mid‐trial, and post‐trial periods. These focus on prospectively allocating sufficient resources for orderly closeout; developing dyad‐centered communication plans; helping dyads build and maintain support networks; and, if a trial stops, informing dyads rapidly. Participants and study partners invest time, effort, and hope in their research participation. The research community should take intentional steps toward better communicating with and supporting participants when clinical trials end early. The Participant FIRST recommendations are a practical guide for embarking on that journey.
Semiconducting Copolymers with Naphthalene Imide/Amide π‐Conjugated Units: Synthesis, Crystallography, and Systematic Structure‐Property‐Mobility Correlations
Authors: Chen Yao , Wu Jianglin , Lu Shirong , Facchetti Antonio , Marks Tobin J. 
Published: August 2022

AbstractIn a series of n‐type semiconducting naphthalene tetracarboxydiimide (NDI)‐dithiophene (T2) copolymers, structural and electronic properties trends are systematically evaluated as the number of NDI carbonyl groups is reduced from 4 in NDI to 3 in NBL (1‐amino‐4,5‐8‐naphthalene‐tricarboxylic acid‐1,8‐lactam‐4,5‐imide) to 2 in NBA (naphthalene‐bis(4,8‐diamino‐1,5‐dicarboxyl)‐amide). As the NDI‐T2 backbone torsional angle falls the LUMO energy rises. However, the thienyl attachment regiochemistry also plays an important role in less symmetric NBL and NBA. Electron mobility is greatest for N2200 (0.17 cm2 V−1 s−1) followed by PNBL‐3,8‐T2 and PNBA‐2,6‐T2 (0.11 cm2 V−1 s−1), 0.02 cm2 V−1 s−1 in PNBL‐4,8‐T2, and negligible in PNBA‐3,7‐T2. Charge transport reflects a delicate balance between electronic backbone communication (optimum for N2200 and PNBL‐4,8‐T2), backbone planarity (optimum for PNBA‐2,6‐T2 and PNBL‐3,8‐T2), LUMO energy (optimum for N2200), π–π stacking distance (optimum for PNBA‐2,6‐T2), and film crystallinity (optimum for PNBA‐2,6‐T2 and N2200). These results offer generalizable insight into semiconducting copolymer design.
Single‐Step Synthesis of Atropisomers with Vicinal C−C and C−N Diaxes by Cobalt‐Catalyzed Atroposelective C−H Annulation
Authors: Wang Bing‐Jie , Xu Guo‐Xiong , Huang Zong‐Wei , Wu Xu , Hong Xin , Yao Qi‐Jun , Shi Bing‐Feng 
Published: August 2022

AbstractThe atroposelective synthesis of atropisomers with vicinal diaxes remains rare and challenging, due to the steric influence between the two axes and their unique topology. Herein, we disclose a single‐step construction of atropisomers with vicinal C−C and C−N chiral diaxes by cyclopentadiene (Cp)‐free cobalt‐catalyzed intramolecular atroposelective C−H annulation, providing the desired diaxial atropisomers of unique structures with decent stereocontrols of both axes (up to >99 % ee and 70 : 1 dr). The optically pure products bearing fluorophores show circular polarized luminescence (CPL) properties, being candidate materials for potential CPL applications. Atropisomerization experiments and density function theory (DFT) calculations are conducted to study the rotational barriers and rotation pathways of the diaxes.
Ring‐to‐Thread Chirality Transfer in [2]Rotaxanes for the Synthesis of Enantioenriched Lactams
Authors: Lopez‐Leonardo Carmen , Saura‐Sanmartin Adrian , Marin‐Luna Marta , Alajarin Mateo , Martinez‐Cuezva Alberto , Berna Jose 
Published: August 2022

AbstractThe synthesis of chiral mechanically interlocked molecules has attracted a lot of attention in the last few years, with applications in different fields, such as asymmetric catalysis or sensing. Herein we describe the synthesis of orientational mechanostereoisomers, which include a benzylic amide macrocycle with a stereogenic center, and nonsymmetric N‐(arylmethyl)fumaramides as the axis. The base‐promoted cyclization of the initial fumaramide thread allows enantioenriched value‐added compounds, such as lactams of different ring sizes and amino acids, to be obtained. The chiral information is effectively transmitted across the mechanical bond from the encircling ring to the interlocked lactam. High levels of enantioselectivity and full control of the regioselectivity of the final cyclic compounds are attained.
Bimetallic Catalysis in Stereodivergent Synthesis
Authors: Huo Xiaohong , Li Guanlin , Wang Xi , Zhang Wanbin 
Published: August 2022

AbstractBimetallic catalysis has emerged as an efficient strategy for the development of new chemical reactions. Importantly, this strategy also provides a simple and powerful platform for stereodivergent synthesis, whereby all the possible stereoisomers of products bearing two stereocenters can be easily prepared from the same set of starting materials. In this Minireview, the development of stereodivergent allylic substitution, propargylic substitution, hydrofunctionalization, and annulation based on bimetallic catalysis has been summarized. It is expected that more bimetallic catalytic systems will be developed and applied for the stereodivergent synthesis of valuable molecules.
Kinetic‐Sieving of Carbon Dioxide from Acetylene through a Novel Sulfonic Ultramicroporous Material
Authors: Cui Jiyu , Qiu Zhensong , Yang Lifeng , Zhang Zhaoqiang , Cui Xili , Xing Huabin 
Published: August 2022

AbstractThe engineering and tailoring of porous materials to realize the precise discrimination of CO2 and C2H2, with almost identical kinetic diameters, is a challenging task. We herein report the first example of the kinetic‐sieving of relatively larger molecule of C2H2 from CO2 by a novel sulfonic anion‐pillared hybrid ultramicroporous materials of ZU‐610a. Specifically, ZU‐610 constructed from copper(II), isonicotinic acid and 1,2‐ethanedisulfonic acid is synthesized and shows the preferential affinity for C2H2 over CO2. After the post‐synthetic heat treatment of ZU‐610, ZU‐610a with a contracted aperture is obtained. Interestingly, the C2H2‐selctive ZU‐610 was reversed to the CO2‐selective ZU‐610a. High purity C2H2 (>99.5 %) could be directly obtained from the dynamic breakthrough experiments on an equimolar C2H2/CO2 mixture at 298 K. This study provides guidance for the design of adsorbents aimed at separation systems with similar kinetic diameter.
An Activatable Near‐Infrared Afterglow Theranostic Prodrug with Self‐Sustainable Magnification Effect of Immunogenic Cell Death
Authors: Gao Zhiyuan , Jia Shaorui , Ou Hanlin , Hong Yuning , Shan Ke , Kong Xianglong , Wang Zhiming , Feng Guangxue , Ding Dan 
Published: August 2022

AbstractHerein, we report an activatable near‐infrared (NIR) afterglow theranostic prodrug that circumvents high background noise interference caused by external light excitation. The prodrug can release hydroxycamptothecin (HCPT) in response to the high intratumoral peroxynitrite level associated with immunogenic cell death (ICD), and synchronously activate afterglow signal to monitor the drug release process and cold‐to‐hot tumor transformation. The prodrug itself is an ICD inducer achieved by photodynamic therapy (PDT). PDT initiates ICD and recruits first‐arrived neutrophils to secrete peroxynitrite to trigger HCPT release. Intriguingly, we demonstrate that HCPT can significantly amplify PDT‐mediated ICD process. The prodrug thus shows a self‐sustainable ICD magnification effect by establishing an “ICD‐HCPT release‐amplified ICD” cycling loop. In vivo studies demonstrate that the prodrug can eradicate existing tumors and prevent further tumor recurrence through antitumor immune response.
Synthesis and evaluation of novel almazole D analogs as anticancer agents
Authors: Oluyori Abimbola P. , Harini Tirunagari , Sangu Komal G. , Krishna Eruva Vamshi , Jadav Surender S. , Misra Sunil , Rode Haridas B. 
Published: August 2022

AbstractNovel almazole D–amide conjugates, esters, and N‐alkylated analogs were synthesized and investigated for their anticancer activity against seven cancer cell lines. Among the series, compounds 5g and 5m showed significant anticancer activities against multiple cell lines with moderate selectivity indices. Compound 5g had IC50 values of 5.86 ± 0.31, 9.94 ± 0.06, 12.74 ± 0.12, and 9.40 ± 0.03 μM against the B16‐F10, DU145, HeLa, and LC‐540 cell lines, respectively, while compound 5m showed IC50 values of 6.35 ± 0.09, 9.17 ± 0.11, 9.00 ± 0.011, 19.65 ± 0.63, 8.13 ± 0.04, and 11.56 ± 0.01 μM against B16‐F10, DU145, HeLa, HepG2, LC‐540, and SK‐BR‐3 cells, respectively. Compared to almazole D, which only showed significant activity against B16‐F10 cells (IC50 = 9.05 ± 0.008 μM), the synthesized analogs showed improved anticancer activity against multiple cell lines. The kinase inhibition assay coupled with the docking studies revealed that epidermal growth factor receptor (EGFR) kinase inhibition via interaction with amino acid residue T790 on the EGFR is one of the possible mechanisms by which 5g exerts its anticancer potential. The ADMET prediction and drug‐likeness of the analogs project the synthesized analogs as promising agents, which can be further developed for application in cancer therapy.
Bioproducts from proteins in neem seed oil meals
Authors: Aramwit Pornanong , Shubashree K. R. , Nagananda G. S. , Reddy Roopa , Kavya S. , Reddy Narendra 
Published: July 2022

AbstractExtraction of oil from Azadirachta indica generates oil cake (meal) containing proteins and carbohydrates. The proteins in neem oil cake with antimicrobial and antioxidant properties have been made into films and studied for their properties and potential applications. Neem oil cakes are available in large quantities at low cost but are non‐edible and have limited applications. Currently, most of the neem oil cakes are used as fertilizers. Neem oil cakes contain about 25–30% proteins and 30–40% carbohydrates, which are valuable. Proteins in the neem oil cake could be suitable for food, medical, biotechnology, and other applications. However, there are no reports on the properties or applications of proteins in neem meal. In this study, a simple alkaline process was used to extract proteins from the oil meal and the proteins obtained were converted into films. The films were crosslinked with citric acid (CA) to improve mechanical properties and stability in aqueous conditions. Neem protein films have good resistance to aqueous conditions and acceptable strength and elongation. The films have better antifungal activity than antibacterial activity. Cells (L929) were able to attach and grow on the films but at a lower rate than Dulbecco's Modified Eagle Medium (DMEM) media. Proteins in neem oil cake show potential as substitutes for the animal‐based proteins in current use. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.