bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influencia de la cocina fusión en la ampliación de la oferta gastronómica en la ciudad de Portoviejo
Authors: Bermúdez-Cevallos Gema , Valdés-Pérez Dayana 
Published: September 2022

La oferta gastronómica de un país no solo representa su cultura, sino que contribuye a su desarrollo turístico. El objetivo de esta investigación fue analizar la influencia de la cocina fusión en la ampliación de la oferta del turismo gastronómico local en la ciudad de Portoviejo, provincia de Manabí. El estudio fue del tipo descriptivo y de enfoque mixto no experimental, se aplicaron instrumentos de recolección de datos como fichas de observación, encuestas, entrevistas y el análisis FODA. Entre los principales resultados desatacan la oferta de alrededor de 22 platos de cocina fusión, asentados en el fichaje realizado el primer semestre del año 2022, con una base de ingredientes típicos de la localidad, como maní, plátano, cereales y mariscos. Son siete los restaurantes que promueven este tipo de cocina a través de la creación e implementación de platos innovadores en la oferta culinaria, los cuales gozan de una considerable aceptación de la población local. Si embargo, la investigación demostró que la cocina fusión constituye una alternativa viable para potenciar los productos culinarios innovadores que oferta la ciudad de Portoviejo, porque le dan realce a su designación de ciudad creativa en el ámbito gastronómico, pero aún no está completamente posicionada en el mercado turístico, debido a varios factores, entre los que se destacan, la gestión administrativa local.
Palabras Clave: Cocina fusión, desarrollo turístico, cocina creativa, innovación gastronómica.
Estrategia de marketing digital para mejorar la captación de clientes en empresas de ventas directas en el Cantón Manta, Manabí-Ecuador
Authors: Arreaga-Arroyo Erika , Alvarez-Tituano Vicente 
Published: September 2022

En el presente trabajo se busca conocer las acciones, retos y limitantes en el manejo de estrategias de marketing digital para mejorar la captación de clientes en empresas de venta directa en el cantón Manta perteneciente a la provincia de Manabí. Para lo cual se realizó un análisis cualitativo y cuantitativo mediante la aplicación de entrevistas y encuestas, identificándose, que el factor común de dichas empresas es manejar estrategias SEO, SEM y especialmente las estrategias SMM, las cuales son desarrolladas explícitamente a través de redes sociales. Además, se destaca que para el desarrollo de dichas estrategias en su mayoría lo han realizado sin previa planificación o preparación profesional a causa de que observan una mayor captación mediante el marketing digital, priorizado por la red social Facebook y su publicidad pagada. Concluyendo que es fundamental reestructurar y replantear la gestión de estrategias de marketing digital e implementar otras como inbound marketing, email marketing y marketing móvil.
Palabras claves: estrategias SEO, estrategias SEM, estrategias SMM, marketing digital, venta directa.
NOVEL METHOD FOR QUANTIFYING HEPATIC STEATOSIS IN PATIENTS WITH METABOLICALLY ASSOCIATED FATTY LIVER DISEASE
Authors: Borsukov A. , Venidiktova D. , Borsukov S. 
Published: September 2022

Introduction.The article presents an overview of novel complex algorithm based on methods of ultrasound steatometry, combined elastography and dual-energy X-ray absorbtiometry in “Whole body” mode in diagnostic and monitoring of metabolically associated fatty liver disease.The purpose of the study:to assess the possibility of quantitative ultrasound steatometry using in the detection and monitoring of metabolically associated fatty liver disease.Material and methods.157 patients were examined. The main group consisted of 47 patients with liver steatosis; 45 patients with steatohepatitis and clinically insigniﬁcant liver ﬁbrosis (F0-F1); 32 patients with steatohepatitis and clinically signiﬁcant liver ﬁbrosis (F2-F3); 33 patients with focal hepatic steatosis. We used complex algorithm based on methods of questionnaires, laboratory tests, ultrasound steatometry, combined elastography and dual-energy x-ray absorbtiometry in “Whole body” mode, liver biopsy. to determine the severity of steatosis, a scale was used: S0 – no steatosis; 2.9 dB/cm.Results and conclusion.It is possible to use quantitative ultrasound steatometry for metabolically associated fatty liver disease, as a reference method both for the initial detection of the disease and for monitoring non-drug treatment (sensitivity 90.7%, speciﬁcity 92.4%). The optimal complex for the diagnosis and monitoring of non-drug treatment of metabolically associated fatty liver disease includes an assessment of the level of compliance, the use of quantitative ultrasound steatometry and dual-energy X-ray absorptiometry in the «Whole body» mode (sensitivity 92.8%, speciﬁcity 92.3%).
МЕТОДОЛОГІЧНІ ЗАСАДИ РОЗРОБКИ МЕТОДИК РОЗСЛІДУВАННЯ НОВИХ (НЕ ПІДКРІПЛЕНИХ ПРАКТИКОЮ) ВИДІВ КРИМІНАЛЬНИХ ПРАВОПОРУШЕНЬ
Authors: Kоваленко A.B. 
Published: September 2022

Статтю присвячено формулюванню окремих методологічних засад розробки ме­тодик розслідування нових кримінальних правопорушень. Сформульовано концептуальне питання щодо можливості та доцільності розробки вченими-криміналістами методик розслідування нових (не підкріплених практикою) видів кримінальних правопорушень. З’ясовано, що основу окремих методик розслідування складають положення «базової» методики, закріплені в КК України суттєві ознаки розслідуваного виду кримінальних правопорушень та емпіричні дані. Наголошено, що положення «базової» методики широко висвітлені у спеціальній літературі та достатньо пристосовані до адаптації на рівні окремих методик, зокрема й щодо нових видів кримінальних деліктів. Указано, що задля побудови кри­міналістичної характеристики, розробникам методик розслідування нових видів кримінальних правопорушень вірогідно доведеться самостійно здійснювати кримінально-правовий аналіз відповідних новел КК України.
Автор вважає, що роль емпіричних даних під час розробки подібних методик можуть відігравати наявна у відкритих джерелах інформація про розслідувані діяння, практика розслідування суміжних кримінальних правопорушень та передова практика розслідування таких нових деліктів. За відсутності належної емпіричної бази критеріями типізації слідчих ситуацій розслідування можуть виступити інформаційний критерій, передбачені складом правопорушення форми та способи його вчинення, загальні (зовнішні) умови досудового розслідування тощо. Доведено, що учені-криміналісти можуть та мають швидко реагувати на виникнення нових складів кримінальних правопорушень та розробляти відповідні методики їх розслідування. З огляду на відсутність сформованої судово-слідчої практики, а відтак і звичної емпіричної бази для досліджень, розробка окремих методик розслідування нових видів кримінальних правопорушень має здійснюватися із застосуванням специфічного методологічного інструментарію, який потребує подальшого розроблення та висвітлення у спеціальній літературі.
COVID-19 and arrhythmia: The factors associated and the role of myocardial electrical impulse propagation. An observational study based on cardiac telemetric monitoring
Authors: Cozzolino Domenico , Romano Ciro , Nevola Riccardo , Marrone Aldo , Umano Giuseppina R. , Cuomo Giovanna , Rinaldi Luca , Adinolfi Luigi E. ,   Vanvitelli COVID Collaborators
Published: September 2022

BackgroundThe heart is commonly involved in COVID-19, and rhythm disorders have been largely reported.ObjectiveTo evaluate the association of some non-cardiac and cardiac comorbidities and QT dispersion with arrhythmias and their impact on outcomes in hospitalized patients with COVID-19.MethodsEach patient underwent cardiac telemetry monitoring through the entire hospitalization period, laboratory analyses, 12-lead ECG, and lung imaging examination. Patients with arrhythmia were divided into three groups (bradyarrhythmias, tachyarrhythmias, and tachy- and bradyarrhythmias).ResultsTwo-hundred patients completed the study (males, 123; mean age, 70.1 years); of these, 80 patients (40%) exhibited rhythm disorders on telemetry. Patients with arrhythmia were older (p &lt; 0.0001), had a greater number of comorbidities (p &lt; 0.0001), higher values of creatinine (p = 0.007), B-type natriuretic peptide (p &lt; 0.0001), troponin (p &lt; 0.0001), C-reactive protein (p = 0.01), ferritin (p = 0.001), D-dimer (p &lt; 0.0001), procalcitonin (p = 0.0008), QT interval (p = 0.002), QTc interval (p = 0.04), and QTc dispersion (p = 0.01), and lower values of sodium (p = 0.03), magnesium (p = 0.04), glomerular filtration rate (p &lt; 0.0001), and hemoglobin (p = 0.008) as compared to patients without arrhythmia. By comparing the three subgroups of patients, no significant differences were found. At multivariate analysis, age [odds ratio (OR) = 1.14 (95% CI: 1.07–1.22); p = 0.0004], coronary artery disease [OR = 12.7 (95% CI: 2.38–68.01); p = 0.005], and circulating troponin [OR = 1.05 (95% CI: 1.003–1.10); p = 0.04] represented risk factors independently associated with arrhythmia. All-cause in-hospital mortality was ∼40-fold higher among patients with arrhythmia [OR = 39.66 (95% CI: 5.20–302.51); p = 0.0004].ConclusionArrhythmias are associated with aging, coronary artery disease, subtle myocardial injury, hyperinflammatory status, coagulative unbalance, and prolonged QTc dispersion in patients with COVID-19, and confer a worse in-hospital prognosis. Given its usefulness, routinary use of cardiac telemetry should be encouraged in COVID wards.
PD-1 blockage combined with vaccine therapy can facilitate immune infiltration in tumor microenvironment of Lynch syndrome colon cancer
Authors: Ye Kai , Zhong Wenjin , Wang Pengcheng , Chen Yanxin , Chi Pan 
Published: September 2022

Background: Lynch syndrome is a genetic disease resulting from mismatch repair gene mutation. Vaccine therapy can enhance the immunogenicity of Lynch syndrome and improve the therapeutic efficacy of immunotherapy. However, there is no approved Lynch syndrome vaccine coming onto the market.Methods: Herein, we used gene knockdown method to construct Lynch syndrome cell model, paving way for us to develop Lynch syndrome tumor lysate vaccine. Then the isograft technique was employed for constructing the tumor-bearing mouse model of Lynch syndrome. And this isograft model was treated with PD-1 monoclonal antibody and tumor vaccine, respectively. Flow cytometry was used for detecting the proportion of immune cells and immunosuppressive cells, and ELISA was used for detecting the contents of chemokines and cytokines in the blood circulation system and tumor tissues of mice. Finally, IHC was used to detect the effects of tumor vaccines as well as PD-1 antibody on tumor tissue proliferation and angiogenesis.Results: The results demonstrated that tumor vaccine could prolong the overall survival of mice, and improve the disease-free survival rate of mice. The vaccine could increase the proportion of inflammatory cells and decrease the proportion of anti-inflammatory cells in the blood circulation system of mice. In addition, tumor vaccine could also improve inflammatory infiltration in the tumor microenvironment and reduce the proportion of immunosuppressive cells. The results of IHC showed that tumor vaccine could inhibit angiogenesis and tumor cell proliferation in mouse tumor tissues.Conclusion: In colon cancer associated with Lynch syndrome, tumor vaccine can hinder the growth of tumor cells, and assist immunotherapy whose therapeutic effect on this kind of cancer is thus enhanced.
Necroptosis-related lncRNA in lung adenocarcinoma: A comprehensive analysis based on a prognosis model and a competing endogenous RNA network
Authors: Mao Fuling , Li Zihao , Li Yongwen , Huang Hua , Shi Zijian , Li Xuanguang , Wu Di , Liu Hongyu , Chen Jun 
Published: September 2022

Background: Necroptosis, an innovative type of programmed cell death, involves the formation of necrosomes and eventually mediates necrosis. Multiple lines of evidence suggest that necroptosis plays a major role in the development of human cancer. However, the role of necroptosis in lung adenocarcinoma (LUAD) remains unclear. In this study, we aimed to construct an NRL-related prognostic model and comprehensively analyze the role of NRL in LUAD.Methods: A necroptosis-related lncRNA (NRL) signature was constructed in the training cohort and verified in the validation and all cohorts based on The Cancer Genome Atlas database. In addition, a nomogram was developed. The tumor microenvironment (TME), checkpoint, human leukocyte antigen, and m6A methylation levels were compared between low-risk and high-risk groups. Then, we identified five truly prognostic lncRNAs (AC107021.2, AC027117.1, FAM30A, FAM83A-AS1, and MED4-AS1) and constructed a ceRNA network, and four hub genes of downstream genes were identified and analyzed using immune, pan-cancer, and survival analyses.Results: The NRL signature could accurately predict the prognosis of patients with LUAD, and patients with low risk scores were identified with an obvious “hot” immune infiltration level, which was strongly associated with better prognosis. Based on the ceRNA network, we postulated that NRLs regulated the TME of patients with LUAD via cyclin-dependent kinase (CDK) family proteins.Conclusion: We constructed an NRL signature and a ceRNA network in LUAD and found that NRLs may modulate the immune microenvironment of LUAD via CDK family proteins.
Cytochrome P450 1A1 is essential for the microbial metabolite, Urolithin A-mediated protection against colitis
Authors: Ghosh Sweta , Moorthy Bhagavatula , Haribabu Bodduluri , Jala Venkatakrishna Rao 
Published: September 2022

BackgroundCytochrome P450 Family 1 Subfamily A Member 1 (CYP1A1) pathway, which is regulated by aryl hydrocarbon receptor (AhR) plays an important role in chemical carcinogenesis and xenobiotic metabolism. Recently, we demonstrated that the microbial metabolite Urolithin A (UroA) mitigates colitis through its gut barrier protective and anti-inflammatory activities in an AhR-dependent manner. Here, we explored role of CYP1A1 in UroA-mediated gut barrier and immune functions in regulation of inflammatory bowel disease (IBD).MethodsTo determine the role of CYP1A1 in UroA-mediated protectives activities against colitis, we subjected C57BL/6 mice and Cyp1a1-/- mice to dextran sodium sulphate (DSS)-induced acute colitis model. The phenotypes of the mice were characterized by determining loss of body weight, intestinal permeability, systemic and colonic inflammation. Further, we evaluated the impact of UroA on regulation of immune cell populations by flow cytometry and confocal imaging using both in vivo and ex vivo model systems.ResultsUroA treatment mitigated DSS-induced acute colitis in the wildtype mice. However, UroA-failed to protect Cyp1a1-/- mice against colitis, as evident from non-recovery of body weight loss, shortened colon lengths and colon weight/length ratios. Further, UroA failed to reduce DSS-induced inflammation, intestinal permeability and upregulate tight junction proteins in Cyp1a1-/- mice. Interestingly, UroA induced the expansion of T-reg cells in a CYP1A1-dependent manner both in vivo and ex vivo models.ConclusionOur results suggest that CYP1A1 expression is essential for UroA-mediated enhanced gut barrier functions and protective activities against colitis. We postulate that CYP1A1 plays critical and yet unknown functions beyond xenobiotic metabolism in the regulation of gut epithelial integrity and immune systems to maintain gut homeostasis in IBD pathogenesis.
Higher dietary vitamin D intake is associated with better survival among older women: Results from the French EPIDOS cohort
Authors: Gautier Jennifer , Riou Jérémie , Schott Anne-Marie , Blain Hubert , Rolland Yves , Saulnier Patrick , Annweiler Cédric 
Published: September 2022

BackgroundHypovitaminosis D, a condition highly common among older adults, is associated with 35-percent increased all-cause mortality. In contrast, vitamin D supplementation prevents all-cause mortality. The possible role of the dietary intake of vitamin D on mortality remains yet unknown.ObjectivesThe objective of this prospective study was to determine all-cause mortality risk according to baseline dietary vitamin D intake among older adults while accounting for potential confounders including dietary calcium intake.MethodsVitamin D and calcium dietary intakes were estimated at baseline from a self-administered food frequency questionnaire among 3,066 community-dwelling older women aged ≥75 years, recruited in the French EPIDOS cohort between 1992 and 1994, and for whom information about vital status was available in 2010. Dietary vitamin D and calcium intakes were defined as low if &lt;400 IU/day or &lt;1,200 mg/day, respectively.ResultsThe mean ± SD age of the whole cohort was 80.1 ± 3.6 years at baseline. The median survival time from baseline for participants with low dietary vitamin D intake was 11.5 years [95% confidence interval (CI): 11.0–11.9] vs. 12.2 years (95% CI: 11.7–12.9) for those consuming more than 400 IU/day (p = 0.003). Among those with calcium dietary intake &lt;1,200 mg/day, a vitamin D consumption of 400 IU/day and over had a significant positive effect on all-cause mortality (RR: 0.86, p &lt; 0.05). However, no association was retrieved between dietary vitamin D intake and all-cause mortality among participants with dietary calcium intake ≥1,200 mg/day.ConclusionHigher dietary vitamin D intake was associated with better survival in the study cohort, specifically among those consuming &lt;1,200 mg/day of dietary calcium.
PALB2 as a factor to predict the prognosis of patients with skull base chordoma
Authors: Xiong Yujia , Li Mingxuan , Shen Yutao , Ma Tianshun , Bai Jiwei , Zhang Yazhuo 
Published: September 2022

ObjectiveThis study aimed to study the role of PALB2 on the prognosis of skull base chordoma patients and the proliferation, migration, and invasion of chordoma cells. Methods187 patients with primary skull base chordoma were involved in the study. Immunohistochemical analysis was used to measure the PALB2 protein expression. Kaplan-Meier analysis, univariate and multivariate Cox analysis were used to evaluate the impact of PALB2 on patient prognosis. A nomogram was established for predicting the progression free survival of chordoma patients. Cell counting kit-8, colony formation, transwell migration, and invasion assays were used to assess the proliferation, migration, and invasion of chordoma cells with PALB2 knockdown. TIMER 2.0 was used to explore the expression and prognostic role of PALB2 in cancers.ResultsHigh PALB2 expression indicated an adverse prognosis in chordoma. A nomogram involved PALB2, degree of resection, pathology, and Al-mefty classification could accurately predict the progression free survival of chordoma patients. The proliferation, migration, and invasion of chordoma cells significantly decreased after PALB2 knockdown. Additionally, PALB2 showed high expression in various cancers and was associated with a poor prognosis.ConclusionIn summary, our results reveal that high PALB2 expression indicates a poor prognosis of chordoma patients and promotes the malignant phenotypes of chordoma cells in vitro.
The role of dual antiplatelets in geographic atrophy secondary to non-neovascular aged-related macular degeneration
Authors: Chantarasorn Yodpong , Smitthimathin Warin , Vorasayan Pongpat 
Published: September 2022

BackgroundTo evaluate the effects of dual antiplatelets on progression of geographic atrophy (GA) secondary to age-related macular degeneration (AMD), and to determine additional factors predicting rapid GA growth.Material and MethodsIn this retrospective cohort study, patients with unifocal GA were consecutively enrolled (one eye per patient) from 2018 to 2021. The patients were categorized as 1. those receiving dual antiplatelet therapy containing a daily dose of 75 mg clopidogrel plus 81 mg aspirin (DAPT group), and 2. those not receiving DAPT (control group). Areas of GA, based on red-filtered fundus autofluorescence, were measured at baseline, and at 3, 6, and 12 months. The primary outcome was absolute 12-month changes in the square root (SQRT) area.ResultsOne eye in each group developed neovascular AMD and was excluded from the analysis. The DAPT (24 eyes) and control (22 eyes) groups had comparable age and baseline SQRT area (1.2 ± 0.27 and 1.8 ± 0.41 mm, respectively; p adjusted for age = 0.23). At 12 months, after controlling for age and the presence of soft drusen or reticular pseudodrusen, patients receiving DAPT had fewer changes in the SQRT area than that of the control group (0.097 vs. 0.17 mm; p = 0.02). The presence of drusen significantly predicted increased GA growth and choroidal thickness reduction.ConclusionsRoutine uses of dual antiplatelets were associated with decelerating GA growth. Drusen-associated GA may represent a generalized form of choroidal vascular alterations.
Teaching fiberoptic-assisted tracheoscopy in very low birth weight infants: A randomized controlled simulator study
Authors: Wolf Monika , Seiler Berenike , Vogelsang Valentina , Sydney Hopf Luke , Moll-Koshrawi Parisa , Vettorazzi Eik , Ebenebe Chinedu Ulrich , Singer Dominique , Deindl Philipp 
Published: September 2022

ObjectiveWe developed a fiberoptic-assisted tracheoscopy (FAST) method to avoid direct laryngoscopy during surfactant replacement therapy and compared two training approaches on a very low birth weight (VLBW) infant simulator.DesignThis prospective randomized controlled study was conducted at the Department of Neonatology and Pediatric Intensive Care Medicine of the University Medical Center Hamburg-Eppendorf, Germany.ParticipantsWe recruited physicians, trainees, students, and nurses without prior experience in endoscopic techniques.InterventionsParticipants were assigned randomly to a group that received instructions according to Peyton’s Four-Step Approach and a control group that received standard bedside teaching only.Main outcome measuresPrimary endpoints were the total and the component times required to place the bronchoscope and the method success.ResultsWe recruited 186 participants. Compared with the control group, the Peyton group had a lower mean (±standard deviation) FAST completion time (33.2 ± 27.5 s vs. 79.5 ± 47.9 s, p &lt; 0.001; d = 1.12) and a higher FAST success rate (95% vs. 84%, p = 0.036, V = 0.18).ConclusionAfter standardized training, the vast majority of novices completed FAST successfully. Peyton’s four-step approach resulted in faster and more successful performance than standardized training.
Theoretical and Experimental Investigations on the Ultra-Low-Frequency Broadband of Quasi-Static Metamaterials
Authors: Liu Haixia , He Weitao , Li Lixia , Jia Qi 
Published: September 2022

This paper proposes an I-shaped radial elastic metamaterial with ultra-low-frequency broadband characteristics and studies the propagation characteristics of elastic waves in their quasi-static state. Through the calculation of the dispersion relationship, the frequency response function, and the eigenmode displacement field, it is found that the ultra-low-frequency wide band gap can be generated in the quasi-static metamaterial. The wide band gap is mainly caused by modal transitions. The equivalent mass–spring model reveals the modal changes of the I-shaped radial elastic metamaterial under the surface constraints. Furthermore, by studying the directional vibration displacement field of the finite period structure, it is demonstrated that the mechanism of the ultra-low-frequency broadband (0<Reduced frequency(Ω)<0.20) is the local resonance mechanism. Subsequently, the influence of the geometric and the material parameters on the location and width of the band gap is explored numerically. Finally, based on the model, through the hammer modal experiment, it is proven that the quasi-static structure yields an ultra-low-frequency stop band of 0.1–1012 Hz. The research conclusions can be applied to mechanical engineering fields such as ultra-low-frequency vibration reduction.