bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative evaluation of accuracy of the multiple methods of heatstroke risk model including time-stratified case-crossover analysis
Authors: Nemoto Mio , Hirabayashi Satoshi , Sato Seisho , Fujii Akihiro , Nojiri Hidetoshi , Ihara Tomohiko 
Published: July 2022

Abstract
Background
High temperatures in urban areas owing to climate change and urban heat islands have led to an increase in the number of heatstroke patients. To prevent heatstroke, accurate heatstroke patient prediction model should be used to predict and alert people to their risk. However, most previous models have not tested sufficient training data, although susceptibility to heatstroke is likely to be dependent on year-wise trends and is susceptible to training data. We investigated an accurate heatstroke risk model that is robust to the training data. By examining the factors affecting the accuracy and trade-off between the quantity and quality of adding old data to training data, the method of selecting training data for constructing an accurate model was also investigated.
Method
We compared the accuracies of three methods: multiple regression analysis (MR), generalized additive model (GAM), and time-stratified case-crossover analysis (TC). The training data for all combinations from 2012 were tested. By comparing the errors of each method, we identified the error influencing factors in the training data.
Results
The TC errors were the smallest (p < 0.005) and much less sensitive to the training data than others. Best accuracy odds ratios were 1.41–1.44. The error was significantly larger when the number of extremely hot days differed between the training and test data in MR and GAM (p < 0.01, p < 0.05). All three methods tended to increase accuracy up to a certain point and decrease from the middle of the year when adding past years retroactively from the most recent year in training data.
Conclusions
By using the odds ratios produced by TC with low sensitivity to training data, we can develop highly accurate heatstroke risk model that are robust to training data, which has not been possible with previous models. If data are to be included continuously, a model constructed from three or four years of data from the latest one is the most accurate.
Regeneration through somatic embryogenesis from zygotic embryo explant of Boswellia serrata Roxb. and detection of medicinal boswellic acid
Authors: Devikar Sandip Dattu , Naikawadi V. B. , Shirke H. A. , Aher R. K. , Nikam T. D. , Naikawadi Vikas Bandu 
Published: July 2022

Abstract
Boswellia serrata is an important source of medicinal oleo-gum resins and boswellic acid, a unique non-steroidal anti-inflammatory, anti-arthritis drug. This experimental study describes the first time and in vitro direct somatic embryogenesis protocol and plantlet formation from immature zygotic embryos of B. serrata. The morphogenic potential of zygotic embryo was tested on MS medium fortified with cytokinins: 6-furfurylaminopurine [kinetin], N6-benzyladenine [BA], 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)-urea [TDZ], auxins: IAA [Indole-3-acetic acid], NAA [α-naphthaleneacetic acid] and 2,4-D [2,4-dichlorophenoxyacetic acid] alone and in combination. MS medium containing 1mg/l TDZ + 0.50 mg/l 2,4-D + 200 mg/l PVP was effective for inducing direct somatic embryo in 89.63% of cultures. On the surface of the zygotic embryo observed, the globular, heart and cotyledonary stages of somatic embryo development. Better germination and conversion to plantlets (73.2 ± 1.6%) of somatic embryos were observed on subculture to GA3 alone containing medium. The survival of plantlets under ex-vitro conditions was about 72%. Flow cytometry (FCM) confirmed the stable ploidy level in regenerated plantlets. In leaf samples of somatic embryo-derived plantlets, boswellic acid isomers are detected by using UPLC-UV-MS analysis and the content of isomer 3-O-acetyl-11-keto-β-Boswellic acid (AKBA) was 0.13 µg/gm dry weights. The propagation protocol described here provides an important micropropagation technique for this valued plant. Nevertheless, this described propagation method can be used for genetic transformation and medicinally important bioactive boswellic acid production.
Effect of oxytocin massage using oxybelt on self efficacy and breastmilk production among postnatal mother: A quasi experimental study
Authors: Lismidiati Wiwin , Santi Neni Fidya , Widyawati Widyawati 
Published: July 2022

Abstract
Background: Oxytocin hormone is a determinant factor in breastfeeding process. Oxytocin reflex can be stimulated by oxytocin massage. Oxybelt was designed to provide automatic oxytocin massage to postnatal mother. The aim of this study was to identify the effect of oxytocin massage with an oxybelt on self-efficacy and breastmilk production among postnatal mothers.Methods: This was a quasi-experimental study using post design only with control group conducted in one of Public Hospital in Central Java, Indonesia, between November and December 2016. There were 60 mothers recruited by purposive sampling method and divided to control and intervention group. Thirty mothers in the control group used usual oxytocin massage (without additional divice). On the other hand, 30 mothers in the intervention group used oxybelt massage. All mothers were enrolled in three days observation.The effectiveness of these methods were indicated by mother’s self efficacy and breastmilk production.Self Efficacy of postnatal mothers was measured by Breastfeeding Self Efficacy –Short Form and the breast milk productions represented by neonatal’s weight gain, urinary and stool frequencies, sleep duration, and feeding satiety, were assessed using questionnaire. Result: The average score of mother’s self efficacy before and after in the intervention group are 53.20 and 58.13, consecutively. Meanwhile in the control group,it was 49.83 and 56, consecutively.The effect of oxybelt on the breast milk production in the intervention group was shown on neonatal’s higher post feed weight gain, higher number of urination and stools passed per day, longer duration of sleep after breastfeeding, and more satiety than on neonatal in the control group.Conclusion: Statistically, there was no significant difference between control and intervention group in self efficacy outcome,but clinically postnatal mothers in intervention group showed their breastfeeding self efficacy better than control group.Oxybelt massage was effective in improving breastmilk production, thus the oxybelt message can be recommended and applied for all breastfeeding mother.Trial Registration: The study was approved by the Ethical Commision, Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada, Indonesia (IRB number: KE/FK/01/EC/2016) Written informed consent was obtained from each mother before participation.
Investigation of Microplastic Pollution in the Sediment and Commercial Fish Species of Gemlik Bay (Marmara Sea) by Microscopic and Spectroscopic Methods
Authors: Yücedağ Elif , Mülayim Ayşegül , Gündüz Serda Kecel 
Published: July 2022

Abstract
Currently, the microplastic pollution is a common phenomenon in the wold wide. Microplastic density has enhanced and accumulated over decades in the aquatic ecosystems and caused many physiological problems in living organisms. Also, toxicity of microplastics increases by providing a surface area to the other polutants. In this study, we aimed to evaluate and characterize the microplastic pollution in the sediment and commercial fish species in the Gemlik Bay, Marmara Sea. Our results showed that the highest accumulation of microplastics in the sediment is at the station in the Gemport Harbor (9733.3 pieces.kg-1) and the lowest accumulation is at the Kurşunlu offshore (3333.3 pieces.kg-1). On the other hand, the highest microplastic accumulation per individual was in the gills of Synapturichthys kleinii (Risso, 1827) (14.5 pieces.ind-1) and the lowest in Diplodus annularis (Linnaeus, 1758) (0.33 pieces.ind-1). The highest accumulation was indicated (8.75 pieces.ind-1) in the gastrointestinal tract of Chelidonichthys lucerna (Linnaeus, 1758), but the lowest accumulation is in that of D. annularis (0.88 pieces.ind-1) species. The fiber-type particles were the most determined microplastics in sediment and fish samples. Micro-Raman Spectrometer was revealed that Polyvinyl chloride (PVC) and polypropylene (PP) were dominant in the sediment, and polyoxymethylene (POM) and polyphenylene sulfone (PPSU) polymers were dominant in fish species. Our study showed that more intense microplastic pollution were determined in the Gemlik Bay then the other locations of Marmara Sea. Also our results suggested that there was a pollution pressure on the ecosystem and that the requirement of controlling the ports and discharge waters underlined.
Optical Characteristics of the Skin with Dark Circles Using Pump-Probe Imaging
Authors: Hou Yikang , Yang Xiaonan , Huang Lvping , Qi Zuoliang , Xiao Ran 
Published: July 2022

Abstract
SignificancePump-probe imaging was first used for quantitative analysis of melanin in dark circles’ skin to improve the ability to diagnose and treat dark circles on human skin.AimThis study aimed to compare the distribution characteristics in melanin of lower eyelid skin tissues and to determine whether pump-probe imaging has potential for the classification of dark circles in vivo. ApproachSpecimens obtained from 15 patients undergoing blepharoplasty were examined using pump-probe imaging. Furthermore, adjacent slices were respectively treated with hematoxylin–eosin (HE) and ferrous sulfate (FeSO4) staining for cross-references. Subsequently, the melanin content index (MCI) and mean fluorescence intensity (MFI) were quantitatively analyzed by the pump-probe imaging.ResultsThe distribution of melanin granules in the pump-probe image and FeSO4 staining was consistent. Meanwhile, the tissues of the skin with dark circles and normal skin demonstrated significant differences in MCI and MFI. These differences can be used to distinguish the skin with dark circles from the normal skin.ConclusionsNoninvasive pump-probe imaging can distinguish the skin with dark circles from the normal skin, and be used to noninvasively evaluate clinical treatment and track the progress of dark circles or other pigmentation diseases.
Advance Care Planning of Patients Undergoing High-Risk Surgery: A Concept Analysis
Authors: Yamamoto Kanako , Suzuki Miho 
Published: July 2022

Abstract
Background
Many patients undergoing high-risk surgery receive critical care after surgery, yet there is a risk that their condition may deteriorate. Therefore, patients are advised to engage in advance care planning (ACP) by selecting treatment preferences in preparation for potential complications or adverse outcomes. The aim of this research was to clarify the concept of ACP for patients undergoing high-risk surgery.
Methods
The Walker & Avant’s approach to concept analysis was used. The reviewed literature was sourced from PubMed, EMBASE, Ichushi-Web, and CiNii databases as well as guidelines and books. The search was conducted by using keywords that were a combination of the following A, B, and C terms: A, advance care planning, advance directive, or living will; B, critical care or intensive care unit; and C, perioperative.
Results
Five attributes of ACP were extracted: the promise of appropriate treatment, definition of life-prolonging treatment, customization of life-saving care, expression of treatment inclinations and discretion, and continuous plan revision and transition. In addition, two antecedents were extracted: the recognition of expected risks and need for anticipatory decision-making. The three consequences were: respect for autonomy, satisfaction with the decision-making process, and improvement in the quality of care. ACP is characterized by anticipatory decision-making regarding the risks faced by patients. In addition, in the event of a sudden “turn for the worse,” necessary life-saving treatment is guaranteed, and the patient documents their wishes for treatment as well as anxieties and fears that help a medical professional or surrogate decision-maker to select life-prolonging treatment in the event of a complication.
Conclusions
ACP focuses on the quality of life and care rather than determining end-of-life care. The development of this concept contributes to an assessment of the usefulness of ACP support, which can lead to improved approaches to help patients recover over a short period.
Prevalence and risk factors associated with Hepatitis C: A cross-sectional study using data from the Puerto Rico National HIV Behavioral Surveillance System Persons Who Inject Drugs Cycle 5 2018
Authors: Colón-López Vivian , Alvelo-Fernández Paola M. , Centeno-Alvarado Nadia , Salas Ivony Y. Agudelo , Colón Yadira Rolón , Martínez María Pabón , Rodríguez-Lebrón Jorge L. , Reyes-Pulliza Juan C. 
Published: July 2022

Abstract
Background: People Who Inject Drugs (PWID) are at a higher risk of acquiring bloodborne infections. This study aimed to estimate the prevalence of Hepatitis C Virus (HCV) in People Who Inject Drugs (PWID) and identify correlates and risk factors using data from the Puerto Rico National HIV Behavioral System, PWID cycle 5 conducted in 2018.Methods: A total of 502 San Juan Metropolitan Statistical Area participants were recruited through Respondent Driven Sampling method. Sociodemographic, health-related, and behavioral characteristics were assessed. Detection of HCV antibodies was completed after the face-to-face survey. Descriptive and logistic regression analyses were performed using STATA 17™. Results: The prevalence of HCV was 76.5% (95%CI: 70.8%-81.4%). Adjusted multivariate logistic regression models showed that frequent speedball injectors and knowing the HCV serostatus of the last sharing partner were significantly more associated with HCV infection (ORa = 4.17; 95% CI: 1.40-12.42; ORa = 4.49; 95% CI: 1.43-14.09, respectively).Conclusions: A high prevalence of HCV infection in PWID was observed in this sample, validating the continuing call for local action for public health and prevention strategies.
TADOX ® Technology for Open Drains and surface water rejuvenation: Making Habitats Sustainable
Authors: Bahadur Nupur , Bhargava Nipun , Sarkar Shyamal Kumar , Dhawan Vibha 
Published: July 2022

Abstract
Untreated sewage and domestic wastewater flowing through open drains is a huge problem faced by many developing and underdeveloped countries, which poses severe environmental, public health and occupational health hazards. Open drains find route to surface water bodies mainly due to absence of dedicated conveyance of wastewater to centralized or decentralized wastewater treatment facilities. Hence, there is an urgent need for onsite treatment of open drains. In this regard, TADOX® (TERI Advanced Oxidation) Technology from TERI (The Energy and Resources Institute) assumes significance as it provides end-to-end treatment together with small footprint and higher efficiency. It utilizes UV/ TiO2 photocatalysis at the secondary stage of wastewater treatment in an indigenously developed technology. This paper presents a case study of an open drain passing through TERI campus, which when treated with TADOX® Technology, improved physico-chemical and microbiological parameters and degraded some micropollutants. The open drain which could not even meet the Class E of Water Quality Criteria laid down by Central Pollution Control Board (CPCB), Government of India in its untreated form, attained Class A Water Quality Criteria after end-to-end TADOX® Treatment in 5h. It thus opens the possibility of treating open drains and TADOX® as a promising decentralized wastewater treatment (DWWT) technology to rejuvenate surface water bodies, enhancing safe reuse, thereby making habitats sustainable. Thus, such an on-site treatment approach not only helps in achieving United Nations Sustainable Development Goal (SDG) 6, but also contributes towards achieving SDG 3, 11 and 15.
Anisometropia in Children in Shandong, China: A Prospective Longitudinal Study
Authors: Jing Xu , Yuanyuan Hu , Xiuyan Zhang , Zihang Xu , Yi Qu , Tailiang Lv , Jike Song , Hongsheng Bi 
Published: July 2022

Abstract
Purpose
To determine the characteristics of anisometropia in children.
Methods
A total of 1474 children (4–15 years old) from 12 schools of Guanxian county (Shandong, China) were enrolled between December 2018 and June 2020 through cluster sampling. The changes of differences in spherical equivalent refraction (SER), axial length (AL) in different anisometropia, and the increases of SER, AL in new-onset anisometropia, non-anisometropia were evaluated over 18 months.
Results
Among 1474 children, 1347 (91.4%) children had a full set of data at the first visit. The prevalence of SER anisometropia was 8.5% at the first visit, and increased to 15.1% at the second visit (χ2 = 27.077, P < 0.001). Among children with SER anisometropia (n = 110), 25.45% (28/110) experienced an increase in anisometropia ≥ 0.5 D, whereas 20.91% (23/110) experienced a decrease in anisometropia ≥ 0.5 D, and 53.64% (59/110) with anisometropia were stable. For L eyes, the increase of SER was greater in the new-onset anisometropia than that in the non-anisometropia (P < 0.05); for H eyes, the increases of SER in the two groups were not significantly different (P > 0.05).
Conclusions
The prevalence of anisometropia increased over time. Nearly half of children with anisometropia were unstable. The unilaterally greater rate of SER increase in children could be associated with the development of anisometropia.
Molecular mapping of drought responsive QTLs during reproductive stage of rice using a GBS (Genotyping by sequencing) based SNP linkage map
Authors: Roy Nabarun , Verma Rahul Kumar , Modi Mahendra Kumar 
Published: July 2022

Abstract
The reproductive stage is the most important critical stage in rice where water is needed for the formation of seeds. Water scarcity in this situation drastically reduces yield which in turn hampers farmers’ effort towards agriculture. Traditional landraces do have resistant genes inside them against several abiotic and biotic stresses which can act as valuable donors to produce new gene combinations. A high-density SNP based genetic map was constructed by Genotyping by sequencing (GBS) technique using F7 recombinant inbred line (RIL) population developed from Banglami x Ranjit to identify tightly linked markers associated with QTLs. The total map length was 1306.424 cM which had an average inter-marker distance of 0.281 cM. A total of 42 QTLs were identified under both reproductive stage drought stress (RS) and non stress (NS) conditions for sixteen different yield and its component traits which explained a phenotypic variance (PVE) of 1.95-13.36 %. Four QTLs explained a PVE ≥ 10% which denotes they are major QTLs. Analysis of the genes present within the QTL regions revealed the presence of 6 candidate genes which were differentially expressed under stress condition. These genes and QTLs will be helpful for marker-assisted pyramiding to improve drought tolerance in rice.
STING mediates nuclear PD-L1 targeting-induced senescence in cancer cells
Authors: Shin Jeon-Soo 
Published: July 2022

Abstract
Immune checkpoint molecule programmed death-ligand 1 (PD-L1) is overexpressed in cancer cells and imparts resistance to cancer therapy. Although membrane PD-L1 has been targeted for cancer immune therapy, nuclear PD-L1 was reported to confer cancer resistance. Therefore, it is important to regulate the nuclear PD-L1. The mechanisms underlying the therapeutic efficacy of PD-L1 targeting have not been well established. Cellular senescence has been considered as a pivotal mechanism to prevent cancer progression, and recently, PD-L1 inhibition was shown to be involved in cancer cell senescence. However, the relevance of PD-L1 targeting-induced senescence and the role of stimulator of interferon genes (STING) has not been reported. Therefore, we aimed to identify the role of PD-L1 in cancer progression and how it regulates cancer prevention.In this study, we found that PD-L1 depletion induced senescence via strong induction of STING expression in mouse melanoma B16-F10 and colon cancer CT26 cells, and in human melanoma A375 and lung cancer A549 cells. Interestingly, nuclear PD-L1 silencing increased STING promoter activity, implying that PD-L1 negatively regulates STING expression via transcriptional modulation. Furthermore, we showed that PD-L1 binds to the STING promoter region, indicating that PD-L1 directly controls STING expression to promote cancer growth. In addition, when we combined PD-L1 silencing with the senescence-inducing chemotherapeutic agent doxorubicin, the effect of PD-L1-targeting was even more powerful. Overall, our findings can contribute to the understanding of role of PD-L1 in cancer therapy by elucidating a novel mechanism for PD-L1 targeting in cancer cells.
Objective Analysis of Facial Bone Computed Tomography Images Using Curvature Measurement
Authors: Kim Jeenam , Yoo Jung Hwan , Choi Seung Hoon , Ko Kwang Yeon , Choi Hyung Jin , Lee Ki Hyun , Kim Hyung Seok , Lee Myung Chul 
Published: July 2022

Abstract
The human facial skeleton consists of multiple segments, and causes difficulty during analytic processes. We developed image analysis software to quantify the amount of injury and validate the smooth curvature of the surface after facial bone reduction surgery. Three-dimensional computed tomography (CT) images of facial bone were obtained from 40 patients who had undergone open reduction surgery to treat unilateral zygomaticomaxillary fractures. Analytic software was developed based on the discrete curvature of a triangular mesh model. The discrete curvature values were compared before and after surgery using two landmarks. For the inferior orbital rim, the weighted average of curvature changed from 0.543±0.034 to 0.458±0.042. For the anterior maxilla, the weighted average of curvature changed from 0.596±0.02 to 0.481±0.031, showing a significant decrement (P<0.05). The curvature was further compared with the unaffected side using the Bray–Curtis similarity index (BCSI). The BCSI of the inferior orbital rim changed from 0.802±0.041 to 0.904±0.015, and that for the anterior maxilla changed from 0.797±0.029 to 0.84±0.025, demonstrating increased similarity (P<0.05). In computational biology, adequate analytic software is crucial. The newly developed software demonstrated significant differentiation between pre- and postoperative curvature values. Modification of current formulas and software will lead to further advancements.