bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of different feeding methods on growth and slaughter performance, blood biochemical indexes and intestinal morphology in Minxinan black rabbits
Authors: chen dongjin , Sun ShiKun , Chen YanFeng , Wang JinXiang , Sang Lei , Gao ChengFang , Xie Xiping 
Published: August 2022

Abstract
Feed restriction after weaning is a common strategy in commercial rabbit farms to improve feed efficiency, promote health and to reduce mortality. However, no study has been published on Minxinan black rabbits. The effects of feed restriction on growth and slaughter performance, intestinal morphology and serum biochemical indexes of Minxinan black rabbits were evaluated in this study. Rabbits in group A (control group) ad libitum intake, the others in feed restriction groups (groups B, C and D) were restricted by 80% of the average daily feed intake of group A the day before. The experimental period lasted 8 weeks. The results indicated that, compared with group A, diarrhea rate of feed restriction groups C was significantly decreased (P < 0.05), the average daily feed intake (ADFI), feed/gain (F/G), abdominal fat weight, abdominal fat rate, the contents of total protein (TP), albumin (ALB), globulin (GLB), alanine aminotransferase (ALT), Low density lipoprotein (LDL) and intestinal crypt depth of feed restriction groups were significantly reduced (P < 0.01). F/G of group D was significantly lower than that of groups B and C (P < 0.05). Efficiency index (EEI) of groups C and D were higher than of groups A and B (P < 0.01). Triglyceride (TG) of groups C and D were significantly lower than group A (P < 0.01). The V/C of duodenum and jejunum of group D was significantly higher than that of group A (P < 0.01). In conclusion, the F/G, diarrhea rate, abdominal fat rate, serum ALT and lipid indexes of Minxinan black rabbits can be reduced, EI and intestinal health can be improved by feed restriction. Feeding twice a day, 30% at 8:00am and 70% at 4:00pm has the best comprehensive effects.
A prospective, single-centre, randomized, double-blind controlled study protocol to study whether long-term oral metronidazole can effectively reduce the incidence of postoperative liver metastasis in patients with colorectal cancer
Authors: Gao Rui Qi , Mo Zhen Chang , Zhou Hai Kun , Yu Peng Fei , Wang Wei Dong , Dong Dan Hong , Yang Xi Sheng , Li Xiao Hua , Ji Gang 
Published: August 2022

Abstract
Introduction
Fifteen to twenty-five percent of patients with colorectal cancer have combined liver metastases at the time of diagnosis, whereas an additional fifteen to twenty-five percent will develop liver metastases after curative resection of primary colorectal cancer, with the vast majority (80% − 90%) of liver metastases unresponsive to curative resection at first. Colorectal cancer liver metastasis is also the leading cause of death in patients with colorectal cancer. In recent years, several studies have demonstrated that intestinal flora, especially Fusobacterium nucleatum, plays a crucial role in the development of colorectal cancer liver metastasis, so we hypothesized that long-term metronidazole use could effectively reduce the incidence of postoperative liver metastasis in colorectal cancer patients.
Methods/design
This study is a prospective, single-centre, randomized, double-blind controlled study in which 300 patients will be randomly assigned to the test group or the control group in a 1:1 allocation ratio. The aim of this trial is to demonstrate that long-term oral antibiotics can effectively reduce the incidence of postoperative liver metastasis in patients with colorectal cancer.
Ethics and dissemination
Ethics approval was obtained from the Ethics Committee at the Chinese Ethics Committee of Registering Clinical Trials (ChiECRCT20210229). The results of this study will be disseminated at several research conferences and as published articles in peer-reviewed journals.
Trial registration
. ChiCTR2100046201. Registered on July 05, 2021, http://www.chictr.org.cn/edit.aspx?pid=125730&htm=4
Comparative Dosimetric Study of Intensity-modulated Radiotherapy and Volumetric-modulated Arc Therapy Techniques with Hypofractionated Intensity-modulated Radiotherapy after Modified Radical Mastectomy for Breast Cancer
Authors: Lin Hongxiao , Sheng Xuren , Liu Haowu , Zhang Peng , Yin Li , Xu Chengsheng , Ke Xue , Ni Jin , Liu Ke , Yin Yachao , Jiang Xiaoxia , Zhang Yufei , Dai Ying , Liu Yunqin , Zang Chunbao 
Published: August 2022

Abstract
Background
This study aimed to compare the advantages and disadvantages of intensity-modulated radiation therapy (IMRT) and volumetric-modulated arc therapy (VMAT), in left-sided breast cancer patients who underwent hypofractionated intensity-modulated radiotherapy after a modified radical mastectomy.
Methods
For the study, 20 patients with left-sided breast cancer who required adjuvant radiotherapy after a modified radical mastectomy were selected randomly. The specified dose of 43.5Gy/15f was adopted to plan for the IMRT and the VMAT. Dose volume histograms (DVH) were employed to evaluate the dose distribution of planning target volumes and organs at risk in both plans.
Results
The homogeneous index (HI) and conformity index (CI) of the VMAT group were found to be better than those of the IMRT group (P < 0.05). VMAT showed better and more uniform dose distribution in the planning target volumes as well as a lower monitor unit (MU).No significant difference was found in V5 of the affected lung and heart between the two groups (P > 0.05). V10, V20, V30, and Dmean of the affected lung and whole heart V10, V20, V30, V40, Dmean, and Dmax of the VMAT group were better than those of the IMRT group (P < 0.05). Dmean and Dmax of the left anterior descending (LAD) of the heart in the VMAT group were better than those in the IMRT group (P < 0.05), and the VMAT program was effective in reducing cardiopulmonary dose. A significant advantage of V30 and Dmean was found in the VMAT group over the IMRT group (P < 0.05).
Conclusions
Compared with the IMRT program, the VMAT program achieved a higher clinical value since it improved the dose distribution in the target area, reduced the cardiopulmonary dose, protected organs at risk (OAR) (e.g., the thyroid), and shortened the treatment time.
Mechanical activation with Easy Clean enhanced organic tissue removal from simulated internal root resorption.
Authors: MONTEIRO LAISE PENA BRAGA , de Sousa Sérgio Emilio Miranda , de Castro Roberta Fonseca , Silva Emmanuel João Nogueira Leal da , Brandão Juliana Melo da Silva 
Published: August 2022

Abstract
Background: This study aimed to evaluate the effectiveness of passive ultrasonic irrigation (PUI) compared to mechanical activation with Easy Clean in the removal of organic tissue from simulated areas of internal root resorption.Methods: The root canals of 72 extracted single-rooted teeth with oval canals were instrumented with Reciproc R25 instruments. After root canal preparations, the specimens were split longitudinally, and semicircular cavities were prepared using a round bur on each half of the roots. Samples obtained from bovine muscle tissue were weighed and adapted into semicircular cavities. The roots were reassembled and joined, and the teeth were divided into six groups (n=12) according to the irrigation protocol: NaOCl without activation; NaOCl + PUI; NaOCl + Easy Clean; distilled water without activation; distilled water + PUI; and distilled water + Easy Clean. After irrigation protocols, the teeth were disassembled, and the remaining organic tissue was weighed. Data were analyzed by two-way ANOVA and Tukey’s post hoc test (p<0.05).Results: Tissue weight reduction was significantly affected by the activation method (p<0.05) and by irrigation solution (p>0.05). The use of Easy Clean resulted in the greatest tissue weight loss compared to those of PUI and no activation (p<0.05).Conclusions: Mechanical activation with Easy Clean enhanced organic tissue removal from simulated internal resorption more effectively than passive ultrasonic irrigation. Easy Clean for agitation of the irrigating solution is effective in removing simulated organic tissues from artificial internal resorption cavities, being an alternative to the use of passive ultrasonic irrigation
Clinical and bacteriological comparison of mouthwashes used in self-care during perioperative oral management: a randomized clinical trial
Authors: Otagiri Hiroki , Kurita Hiroshi , Yamada Shin-ichi , Sakai Hironori , Tobata Haruko , Yanai Kota , Matsubara Kaya , Eguchi Toru 
Published: August 2022

Abstract
Background: Perioperative oral management reduces the risk of postoperative wound infection and pneumonia. Currently, we recommend gargling with povidone-iodine (PVP-I) to maintain the cleanliness of the oral cavity; however, the bactericidal capability of PVP-I may be reduced by dilution or saliva. Mouthwashes containing cetylpyridinium chloride (CPC) that are used as oral hygiene products to prevent gingivitis are considered less susceptible to dilution and saliva than PVP-I. We conducted a randomized controlled trial to evaluate the clinical and bacteriological effects of CPC mouthwash using PVP-I gargle as a control.Methods: Eighty-three patients who visited the Oral Care Center of Shinshu University Hospital prior to surgery with general anesthesia were included. Patients were assessed for clinical indices and underwent perioperative oral care (POC) comprising scaling and professional mechanical tooth cleaning before surgery. Patients were divided into two groups using CPC mouthwash (n = 42) or PVP-I gargle (n = 41) in addition to brushing. Buccal mucosa tongue and saliva samples were collected before and after POC, immediately before and after surgery, and one week after surgery. All samples were analyzed using 16S rRNA gene sequencing and quantitative polymerase chain reaction.Results: Reduction in dental plaque adhesion (Oral Hygiene Index-Simplified) and gingival bleeding (bleeding on probing), along with improved total bacterial counts were observed in both groups one week post-surgery. The microbial community composition significantly differed between the CPC and PVP-I groups. In the CPC group, Streptococcus was suppressed until one week post-surgery. In the PVP-I group, the genus Streptococcus increased again at one week post-surgery. Linear discriminant analysis effect size analysis showed that Haemophilus and Streptococcus was a characteristic genus of the CPC and PVP-I group, respectively, at one week post-surgery.Conclusion: Our study revealed differences in the effects of CPC mouthwash and PVP-I gargle on the microbial community structure. The CPC mouthwash may maintain the antibiotic effect for a longer period than PVP-I gargle. This result will support data for more detailed research in the future.Trial registration: University Hospitals Medical Information Network Clinical Trials Registry (UMIN-CTR), UMIN000030919. Registered January 21, 2018.Funding: Sunstar Inc.
Preventing surgical site infection after cholecystitis by postoperative antibiotic: A randomized controlled trial study
Authors: Zabihi-Mahmoudabadi Hossein , Akbari Hamed , Abedi-kichi Raziyeh , Rahimpour Ehsan , Hajebi Reza , Tavakkoli Hassan 
Published: August 2022

Abstract
Purpose: Surgical site infection is one of the most common complications of surgical procedures and the best method for its risk reduction is prevention. The main purpose of this study is to assess the prophylactic role of postoperative antibiotics in reducing the incidence of surgical site infection.Methods: We conducted a prospective, randomized trial at Sina Hospital, Tehran, Iran from 2019 to 2021 to compare the postoperative incidence of surgical site infection and deep wound infection, in patients with mild or moderate cholecystitis who underwent elective cholecystectomy in patients receiving prophylactic antibiotics to those who did not receive antibiotics. Results: The postoperative antibiotics do not associated with a significant decrease in postoperative surgical site infection (P>0.05). Conclusion: Through this study, we demonstrated that postoperative antibiotics did not have a prophylactic role in the prevention of surgical site infection.
Physiotherapy intervention on changes in post-chemotherapy sensibility with taxanes
Authors: Rodrigues Maria Thais , Luz Roberta Pitta Costa , Alliz Carmen Sylvia Varella , Reis Thais Andreotti Gelsi , Rizzi Samantha Karlla Lopes de Almeida , Nazário Afonso Pinto , Elias Simone , Facina Gil 
Published: August 2022

Abstract
OBJECTIVE: Evaluate the presence of changes in post-chemotherapy sensibility with Taxane and the efficiency of a physiotherapy intervention that aims at the improvement of this condition. METHODS: We conducted an uncontrolled, longitudinal quantitative and prospective study on 23 patients. Four were not included in the study for not showing changes in sensibility or having a skin condition that rendered the evaluation impossible. The patients responded to the McGill and FACT G-Taxane questionnaires after the sensibility evaluation with a Semmes-Weinstein esthesiometer, an intervention session using a Peridell® therapeutic massager and a subsequent sensibility revaluation. RESULTS: Two regions with the best responses to the intervention were the dermatomes L5 and S1, with initial means of 5.33 and 5.53 and final means of 5.64 and 5.78, respectively (p = 0.012 e 0.020). The general analysis for both superior and inferior limbs showed an increase in the means, with an initial mean of 5.69 and a final mean of 5.81 (p = <0.001). There was a negative correlation between FACT G-Taxane and the McGill Questionnaire (-0.738 e p = <0.001). CONCLUSION: Vibratory stimulus of Peridell® therapeutic massager showed a significant improvement of changes in sensibility on the region of the dermatomes L5 and S1, especially on milder changes. The correlation between FACT G-Taxane and the McGill questionnaire suggests that the more pain the patient feels, there is less life quality and there are more symptoms of toxicity.
IL-1β regulates TRPM2 channel in astrocytes and aggravates their apoptosis during lipopolysaccharide induced cognitive impairment in mice
Authors: Yang Yujiao , Jia Shuaiying , Wang Mingfei , Yun Debo 
Published: August 2022

Abstract
Background: Cognitive dysfunction after sepsis is the most serious complication after discharge of sepsis patients, and its pathogenesis is related to the inflammation of the central nervous system caused by the damaged blood-brain barrier. Interleukin-1β (IL-1β) is a key factor in sepsis-induced cognitive dysfunction in mice. Several investigations have suggested that TRPM2 (transient receptor potential melastatin-2) performs a vital task in immune response and inflammation. The aim of this exploration was to discover the importance of IL-1β in apoptosis in the hippocampal astrocytes of septic mice through in vitro and in vivo assessments. Methods: mouse severe inflammatory was established by intraperitoneal injection of high dose lipopolysaccharide (LPS, 5 mg kg–1), The Morris water maze task and fear conditioning test were used to investigate cognitive functions on the 7th day after LPS injection. Western blotting was implemented to detect the levels of IL-1β , TRPM2 and GFAP. Flow cytometry was employed to ascertain the effects of IL-1β and TRPM2-siRNA on the apoptosis of astrocytes. Immunofluorescent staining was used to examine the expression levels of GFAP and TRPM2 in hippocampus of mice .Results: Intraperitoneal injection of LPS caused cognitive dysfunction in mice, which can be reversed by down-regulation of IL-1β expression in the hippocampus. Elevated expression of IL-1β and TRPM2 were observed in hippocampus from the mouse after LPS injection. Specifc knockdown of IL-1β mRNA expression rescued the elevated expression of TRPM2. Accordingly, specifc knockdown of TRPM2 expression improved the cognitive impairment induced by Intraperitoneal injection of LPS.Conclusions: Elevated expression of IL-1β in the hippocampus may induce cognitive dysfunction by upregulation of TRPM2 in septic mice astrocytes.
Analysis of biofilm expansion rate on agar substrates with different stiffness under limited nutrition
Authors: Wu Jin , Li Xianyong , Kong Rui , Wang Jiankun , Wang Xiaoling 
Published: August 2022

Abstract
In this study, we explore the growth of Bacillus subtilis biofilm on substrates with different agar concentrations, and obtain the area, morphology information and fluorescence intensity of biofilms by using optical imaging technologies. We find that expansion rate variations of biofilms growing on substrates with high and low agar concentration (1.5wt%, 2.0wt%,2.5wt%) are not in the same phase. In the first three days’ growth, the interaction stress between biofilm and each agar substrate increases, which makes the biofilm expansion rate decreases before wrinkle pattern IV (branches) comes up. After three days, in the later growth stage after wrinkle pattern IV appears, the biofilm has larger expansion rate growing on 2.0wt% agar concentration, which has the larger wrinkle distance in wrinkle pattern IV reducing energy consumption. The above findings are further verified through images of the fluorescence intensity of matrix producing cells of each biofilm. Our study shows that the stiff substrate does not always inhibit the biofilm expansion, although it does in the earlier stage, after that, mature biofilms acquire larger expansion rate by adjusting the growth mode through the wrinkle evolution even in nutrient extremely depletion.
Analysis of intraocular amniotic membrane in vitreoretinal disease with complicated retinal detachment - case reports and in-vitro safety assessment.
Authors: Hillenmayer Anna , Wertheimer Christian M. , Gerhard Maximilian-Joachim , Priglinger Siegfried G. , Ohlmann Andreas , Wolf Armin 
Published: August 2022

Abstract
Purpose
Amniotic membrane (AM) is a popular treatment for ocular surface diseases due to its wound healing effects. First intraocular implantations showed good anatomical and functional results. Here, three cases of intravitreal AM transplantation and the morphological and functional outcome are described. The reaction to AM was examined by immunostaining and cellular reactions were investigated on retinal cells in-vitro.
Methods:
Three patients with epiretinal implanted AM during PPV for complicated retinal detachment are presented retrospectively. We investigated the AM-influence in-vitro on RPE cells (ARPE-19), Müller cells (Mio-M1), and retinoblasts (661W). An anti-histone DNA ELISA and live/dead assay for apoptosis, a BrdU ELISA for proliferation and a WST-1 assay for viability were performed. Following AM-removal, tissue-specific cellular responses were evaluated by light microscopy and immunohistochemical staining.
Results:
Despite severe vitreoretinal disease, good anatomical and functional outcomes were achieved in all three cases. Immunostaining of AM showed no immunological rejection. In-vitro, AM mediates no toxic or proliferating effect on RPE and Müller cells but a neuroprotective effect on differentiated retinoblasts.
Conclusion:
AM offers possible advantages treating severe vitreoretinal disease. The pathobiological mode of action was elucidated and we could not determine issues regarding rejection reactions and biocompatibility. Further studies are needed to assess clinical potential.
Appendicular anthropometric changes and poor ECOG performance status is associated with sarcopenia in individuals with Head and neck squamous cell carcinoma.
Authors: Pereira Luciana M B , Ruas Hérika M S , Filho Walter F , Alves Wislene S M , Dias Gabrielle O , Durães Cristina P , Junior Renato S M , Santos Sérgio H S , Farias Lucyana C , Guimarães André L S , Haikal Desirée S , Cardoso Cláudio M , de Paula Alfredo M B 
Published: August 2022

Abstract
Head and neck squamous cell carcinoma (HNSCC) is a prevalent type of cancer with high morbidity and mortality rates worldwide. Sarcopenia, a progressive skeletal muscle wasting disorder, is very prevalent in individuals with cancer and is responsible for poor clinical outcomes. This study investigated the association between demographical, anthropometric, nutritional status, tumor clinical characteristics, physical performance, and C-reactive protein and albumin levels and sarcopenia in individuals with HNSCC (n = 125; male/female ratio: 3.8/1; age: 59.38 ± 10.72 years). Sarcopenia was defined as low muscle strength and low muscle performance. Anthropometrical measurements, nutritional status, performance status, tumor characteristics, and CRP and albumin levels were analyzed using binary logistic regression models. Sarcopenia in individuals with HNSCC was more likely to occur in Individuals that exhibited low MAC (OR = 0.73 IC95% = 0.57-0.93) and MAMC (OR = 11.93, IC95% = 1.76-80.52), and high UFA (OR = 1.22, IC95% = 1.06-1.42) anthropometrical parameters, and ECOG scores > 1 (OR = 4.76, IC95% = 1.55-14.62). Early identification of anthropometric changes in appendicular muscularity and adiposity and the diagnosis of poor performance status tends to favor a prompt identification of sarcopenia in individuals with HNSCC.
Colossal barocaloric effect exploiting amorphous high entropy in solidified polyethylene glycol
Authors: Hu Fengxia , Yu Zibing , Zhou Houbo , Liu Chang , Yuan Shuaikang , Wang Donghui , Hao Jiazheng , Gao Yihong , Wang Yangxin , Wang Bingjie , Tian Zhengying , Lin Yuan , Zhang Cheng , Yin Zhuo , Wang Jing , Chen Yunzhong , Li Yunliang , Sun Jirong , Zhao Tongyun , Shen Baogen 
Published: August 2022

Abstract
Barocaloric effect (BCE) has emerged as one of intense topics aiming at solid-state refrigeration featuring efficiency and cleanness. Materials with solid-liquid phase transition (SL-PT) usually show huge melting entropy but cannot work in full solid-state refrigeration. Here we report colossal barocaloric effect realized by exploiting high entropy inherited from huge disorder of liquid phase in amorphous polyethylene glycol (PEG), which is solidified through introducing 5wt.% polyethylene terephthalate (PET). TEM combined XRD demonstrate the amorphous nature of high-temperature phase after fixed by PET. Although the PEG loses the -OH end mobility in amorphous solid, high entropy still retains owing to retained high freedom of molecule chains. The remained entropy in amorphous PEG is up to 83% of liquid PEG in PEG10000/PET15000, and the barocaloric entropy change reaches ΔSp ∼ 416 J·kg− 1·K− 1 under a low pressure 0.1 GPa, which exceeds the performance of most other BCE materials. Infrared spectra combined with density function theory (DFT) calculations disclose conformational change from liquid to amorphous state, which explains the origin of huge entropy retained and hence colossal BCE in the solidified PEG. This research opens a new avenue for exploring full solid-state barocaloric materials by utilizing genetic high entropy from huge disordering of liquid phase in various materials with SL-PT.