bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of a nomogram for evaluating the prognosis of immunotherapy plus antiangiogenic therapy in non-small cell lung cancer
Authors: Huang Hao , Chen Yao , Weng Xuezi , Li Sirou , Zhang Lin , Chen Peisong 
Published: August 2022

Abstract
Background
With the combination therapy of PD-1/PD-L1 antibody and antiangiogenic drugs used widely in clinic, a novel method to estimate the prognosis of patients is needed. We aimed to develop a nomogram to examine prognosis of anti-PD-1/PD-L1 antibody plus bevacizumab in non-small cell lung cancer (NSCLC) patients.

Methods
We developed a nomogram using the cohort involving 204 NSCLC patients who treated with immunotherapy and anti-angiogenesis therapy. The nomogram was validated under the same conditions in another cohort with 69 patients. Prognostic factors were analyzed by Cox regression analysis. The nomogram was internally validated using bootstrap resampling and then externally validated. Performance was assessed using concordance index, calibration curve and decision curve analysis. Clinical utility was evaluated using receiver operation characteristic curve.

Results
Pleural metastasis (P = 0.001, HR = 2.980, 95%CI 1.521–5.837), ANC (P < 0.001, HR = 5.139, 95%CI 2.081–12.691), ALC (P = 0.010, HR = 0.331, 95%CI 0.142–0.771), B cells (P = 0.005, HR = 0.329, 95%CI 0.151–0.714), Treg cells (P = 0.002, HR = 2.934, 95%CI 1.478–5.826) were independent prognostic factors. The calibration curves showed good consistency and the C-index of nomogram were 0.808, 0.741 in training and external validation cohort, respectively. The area under the curve (AUC) in receiver operation characteristic curves (ROC) are 0.833 (P < 0.001) and 0.908 (P < 0.001), respectively.

Conclusion
We build an accurate and convenient nomogram to predict long-time overall survival (OS) of NSCLC patients treated with PD-1/PD-L1 antibody and antiangiogenic drugs and validated this nomogram. The nomogram might be helpful to clinicians to estimate long-time OS of NSCLC patients treated with PD-1/PD-L1 antibody and antiangiogenic drugs.
Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma
Authors: Zheng Enqi , Cai Zhun , Li Wangyong , Ni Chuandou , Fang Qian 
Published: August 2022

Abstract
Background
To investigate the role of achaete-scute complex-like 2 (ASCL2) in stomach adenocarcinoma (STAD), we analyze whether ASCL2 suppression could retard cancer development and further observe the relevance between ASCL2 and inflammation via Toll-like receptor 4 (TLR4) activation in STAD, both in vitro and in vivo.

Methods
Proliferation, development, inflammation, and apoptosis in STAD are observed using sh-ASCL2 lentivirus via TLR4 activation in vitro and in vivo. The relationship between ASCL2 and inflammation is analyzed. Western blotting of ASCL2 with the target protein of immune-associated cells is performed. The prognosis of STAD and associated ASCL2 mutation are analyzed.

Results
The ASCL2 level in STAD tumor tissues is increased, compared to normal tissues, and brings a worse prognosis. The ASCL2 shows a negative correlation with inflammation, and TLR4 reveals a positive correlation with gastric cancer. ASCL2 expression is high in MGC803 cells. Sh-ASCL2 could reduce STAD development by decreasing proliferation, tumor volume, and biomarker levels and increasing apoptosis in vitro and in vivo. The inflammatory role of ASCL2 is regulated through TLR4 activation. ASCL2 levels may be related to CNTNAP3, CLIP1, C9orf84, ARIH2, and IL1R2 mutations; positively correlated with M2 macrophage and T follicular helper cell levels; negatively correlated with neutrophil, dendritic cell, monocyte, CD8 T cell, and M1 macrophage levels; and involved in STAD prognosis.

Conclusions
The ASCL2 may adjust inflammation in STAD through TLR4 activation and may be associated with related immune cells. ASCL2 is possibly an upstream target factor of the TLR4 signaling pathway.
Efficacy of early goal-directed therapy using FloTrac/EV1000 to improve postoperative outcomes in patients undergoing off-pump coronary artery bypass surgery: a randomized controlled trial
Authors: Tribuddharat Sirirat , Sathitkarnmanee Thepakorn , Ngamsaengsirisup Kriangsak , Sornpirom Sanpicha 
Published: August 2022

Abstract
Background
Early goal-directed therapy (EGDT) using FloTrac reduced length of stay (LOS) in intensive care (ICU) and hospital among patients undergoing coronary artery bypass graft (CABG) with a cardiopulmonary bypass. However, this platform in off-pump CABG (OPCAB) has received scant attention, so we evaluated the efficacy of EGDT using FloTrac/EV1000 as a modality for improving postoperative outcomes in patients undergoing OPCAB.

Methods
Forty patients undergoing OPCAB were randomized to the EV1000 or Control group. The Control group received fluid, inotropic, or vasoactive drugs (at the discretion of the attending anesthesiologist) to maintain a mean arterial pressure 65–90 mmHg; central venous pressure 8–12 mmHg; urine output ≥ 0.5 mL kg−1 h−1; SpO2 > 95%; and hematocrit ≥ 30%. The EV1000 group achieved identical targets using information from the FloTrac/EV1000. The goals included stroke volume variation < 13%; cardiac index (CI) of 2.2–4.0 L min−1 m−2; and systemic vascular resistance index of 1500–2500 dynes s−1 cm−5 m−2.

Results
The EV1000 group had a shorter LOS in ICU (mean difference − 1.3 d, 95% CI − 1.8 to − 0.8; P < 0.001). The ventilator time for both groups was comparable (P = 0.316), but the hospital stay for the EV1000 group was shorter (mean difference − 1.4 d, 95% CI − 2.1 to − 0.6; P < 0.001).

Conclusions
EGDT using FloTrac/EV1000 compared to conventional protocol reduces LOS in ICU and hospital among patients undergoing OPCAB.
Trial registration This study was retrospectively registered at www.ClinicalTrials.gov (NCT04292951) on 3 March 2020.
A case of complex balanced chromosomal translocations associated with adverse pregnancy outcomes
Authors: Luo Yan , Lu Hezhen , Zhang Yanshang , Cui Zhiqiang , Zhang Pingping , Li Yali 
Published: August 2022

AbstractComplex chromosomal rearrangements (CCR) are rare chromosomal structural abnormalities. The chromosomal structural variants in CCR carriers are one of the factors contributing to a history of adverse pregnancy and childbirth. In this study, we report a patient with a history of adverse pregnancy and childbirth who exhibited complex balanced chromosomal translocations. The female patient was phenotypically and intellectually normal; in her first pregnancy, the embryo was damaged, and a histological examination of the chromosomes of the embryos revealed a deletion of approximately 4.66 Mb at 1p32.3p32.2, a duplication of approximately 1.02 Mb at 1p22.2p22.1, a duplication of approximately 1.46 Mb at 6q27 and a deletion of approximately 7.78 Mb at 9p24.3p24.1. Chromosomal examinations of the patient revealed the karyotype to be 46,XX,(1;9)(p32; p34). In the second pregnancy, the foetus was diagnosed prenatally with three or more positive ultrasound soft indicators. The patient's karyotype was re-examined and further confirmed by fluorescence in situ hybridisation as 46,XX,t(1;9;6)(p31;p22;q27), revealing this patient was a carrier of complex balanced chromosomal translocations. Carriers of CCR have a higher risk of spontaneous abortion, and genetic counselling clinicians should consider the karyotype analyses of such patients in clinical practice and recheck their chromosomes if necessary.
Dataset of the de novo assembly and annotation of the marbled crayfish and the noble crayfish hepatopancreas transcriptomes
Authors: Boštjančić Ljudevit Luka , Francesconi Caterina , Rutz Christelle , Hoffbeck Lucien , Poidevin Laetitia , Kress Arnaud , Jussila Japo , Makkonen Jenny , Feldmeyer Barbara , Bálint Miklós , Schwenk Klaus , Lecompte Odile , Theissinger Kathrin 
Published: August 2022

Abstract
Objectives
Crayfish plague disease, caused by the oomycete pathogen Aphanomyces astaci represents one of the greatest risks for the biodiversity of the freshwater crayfish. This data article covers the de novo transcriptome assembly and annotation data of the noble crayfish and the marbled crayfish challenged with Ap. astaci. Following the controlled infection experiment (Francesconi et al. in Front Ecol Evol, 2021, https://doi.org/10.3389/fevo.2021.647037), we conducted a differential gene expression analysis described in (Boštjančić et al. in BMC Genom, 2022, https://doi.org/10.1186/s12864-022-08571-z)

Data description
In total, 25 noble crayfish and 30 marbled crayfish were selected. Hepatopancreas tissue was isolated, followed by RNA sequencing using the Illumina NovaSeq 6000 platform. Raw data was checked for quality with FastQC, adapter and quality trimming were conducted using Trimmomatic followed by de novo assembly with Trinity. Assembly quality was assessed with BUSCO, at 93.30% and 93.98% completeness for the noble crayfish and the marbled crayfish, respectively. Transcripts were annotated using the Dammit! pipeline and assigned to KEGG pathways. Respective transcriptome and raw datasets may be reused as the reference transcriptome assemblies for future expression studies.
Anti-tumor effects of dual PI3K-HDAC inhibitor CUDC-907 on activation of ROS-IRE1α-JNK-mediated cytotoxic autophagy in esophageal cancer
Authors: Jian Zheng , Han Yichao , Zhang Wentian , Li Chengqiang , Guo Wei , Feng Xijia , Li Bin , Li Hecheng 
Published: August 2022

Abstract
Background
PI3K-Akt pathway activation and the expression of histone deacetylases (HDACs) are highly increased in esophageal cancer, suggesting that inhibition of such targets may be a viable therapeutic strategy. Herein, we aimed to evaluate the anti-tumor effect of CUDC-907, a dual PI3K-HDAC inhibitor, in esophageal squamous cell carcinoma (ESCC).

Methods
The anti-tumor effects of CUDC-907 in ESCC were evaluated using cell counting kit-8, flow cytometry, and western blot. mRNA-sequencing was used to explore the mechanism underlying CUDC-907 anti-tumor effects. The relations of reactive oxygen species (ROS), lipocalin 2 (LCN2), and CUDC-907 were determined by flow cytometry, rescue experiments, and western blot. The activation of the IRE1α-JNK-CHOP signal cascade was confirmed by western blot. The in vivo inhibitory effects of CUDC-907 were examined by a subcutaneous xenograft model in nude mice.

Results
CUDC-907 displayed effective inhibition in the proliferation, migration, and invasion of ESCC cells. Through an mRNA-sequencing and functional enrichment analysis, autophagy was found to be associated with cancer cells death. CUDC-907 not only inhibited the PI3K-Akt-mTOR pathways to result in autophagy, but also induced ROS accumulation to activate IRE1α-JNK-CHOP-mediated cytotoxic autophagy by downregulating LCN2 expression. Consistently, the in vivo anti-tumor effects of CUDC-907 accompanied by the downregulated expression of p-mTOR and LCN2 and upregulated expression of p-IRE1α and LC3B-II were evaluated in a xenograft mouse model.

Conclusion
Our findings suggested the clinical development and administration of CUDC-907 might act as a novel treatment strategy for ESCC. A more in-depth understanding of the anti-tumor effect of CUDC-907 in ESCC will benefit the clinically targeted treatment of ESCC.
CDKN2AIP is critical for spermiogenesis and germ cell development
Authors: Cao Yuming , Sun Qi , Chen Zhenlie , Lu Jing , Geng Ting , Ma Ling , Zhang Yuanzhen 
Published: August 2022

Abstract
Background
As a member of RNA-binding protein, CDKN2AIP has been shown to play a critical role in stem cell pluripotency and somatic differentiation. Recent studies indicate that Cdkn2aip is essential for spermatogonial self-renewal and proliferation through the activating Wnt-signaling pathway. However, the mechanisms of how Cdkn2aip regulate spermatogenesis is poorly characterized.

Results
We discovered that the CDKN2AIP was expressed in spermatocyte as well as spermatids and participated in spermiogenesis. Cdkn2aip−/− mice exhibited multiple sperm head defects accompanied by age dependent germ cell loss that might be result of protamine replacement failure and impaired SUN1 expression. Loss of Cdkn2aip expression in male mice resulted in synapsis failure in 19% of all spermatocytes and increased apoptosis due to damaged DNA double-strand break (DSB) repair and crossover formation. In vitro, knockdown of Cdkn2aip was associated with extended S phase, increased DNA damage and apoptosis.

Conclusions
Our findings not only identified the importance of CDKN2AIP in spermiogenesis and germ cell development, but also provided insight upon the driving mechanism.
On dynamic inequalities in two independent variables on time scales and their applications for boundary value problems
Authors: El-Deeb Ahmed A. 
Published: August 2022

AbstractOur work is based on the multiple inequalities illustrated by Boudeliou and Khalaf in 2015. With the help of the Leibniz integral rule on time scales, we generalize a number of those inequalities to a general time scale. Besides that, in order to obtain some new inequalities as special cases, we also extend our inequalities to discrete, quantum, and continuous calculus. These inequalities may be of use in the analysis of some kinds of partial dynamic equations on time scales and their applications in environmental phenomena, physical and engineering sciences described by partial differential equations.
Analysis of the dietary diversity status of agricultural households in the Nkomazi Local Municipality, South Africa
Authors: Sambo T. A. , Oguttu J. W. , Mbombo-Dweba T. P. 
Published: August 2022

AbstractBackgroundDietary diversity is a good alternative measure of the nutritional adequacy and food security. The present study assessed the household dietary diversity status and its determinants among agricultural households in the Nkomazi Local Municipality, South Africa.MethodsOut of 543 households in the study area that were supported by the Phezukomkhono Mlimi programme during the 2018/19 production season, only 355 met the inclusion criteria and consented to participate in this study. The data were analysed using descriptive statistics, computation of the Household Dietary Diversity Score (HDDS) and the ordered logit regression model.ResultsMost of the respondents were elderly (56.10%) and females (59.44%). Most had low levels of education (43.66%) and low farm income (96.34%). In addition, most (65.35%) had large households. The majority (49.86%) of the households in the study area had a HDDS of 4.40, while the median HDDS in the study area was 3.90. Over the recall period, the majority of households consumed cereals (100%) and vegetable (78.31%) food types. Households headed by respondents with no formal education had lower odds (OR = 0.20; 95% CI 0.06–0.61) of having a higher dietary diversity compared to those headed by heads who had attained tertiary education. Households with income ≤ R3000.00 had lower odds (OR = 0.51; 95% CI 0.31–0.85) of having a higher dietary diversity as compared to those with income > R3000.00. Households with 1–5 members had higher odds (OR = 10.41; 95% CI 1.05–103.20) of having a higher dietary diversity as compared to larger households. With every unit increase in the age of the household head, the odds of the household of having a higher dietary diversity increased by 1.03 (OR = 1.03; 95% CI 1.01–1.05).ConclusionSince cereals and vegetables dominated the diets of the respondents, findings of this study highlight the need for improved access to sources of protein among the respondents. Therefore, there is a need for programmes to educate respondents on the dangers of inadequate nutrition (lacking in protein). Strategies that enhance farm income and the level of education among respondents should be prioritised because of their potential to boost Household Dietary Diversity (HDD) in the study area. Collaborations between different stakeholders, such as nutritionists, extension workers and researchers, should be encouraged so as to develop a holistic approach to improving the HDD in the study area.
Evolving etiologies and rates of revision total knee arthroplasty: a 10-year institutional report
Authors: Brown Matthew L. , Javidan Pooya , Early Sam , Bugbee William 
Published: August 2022

Abstract
Background
The number of total knee arthroplasties (TKA) performed in the United States is projected to rise significantly, with a proportionate increase in the revision burden. Understanding the mechanism of failure in primary TKA is important as etiologies continue to evolve and reasons for revision change. The purpose of this study was to determine the reason for revision TKA at our institution among early and late failures and assess if the etiology has changed over a 10-year time-period.

Methods
We identified 258 revision TKAs performed at our institution between 2005 and 2014. Reasons for revision TKA were categorized according to diagnosis. We also conducted subgroup analysis for TKA revisions performed within two years of the primary TKA (early failures) and those performed after two years (late failures). Revision TKAs were also grouped by year of primary TKA (before and after 2000) and time period in which the revision TKA was performed (2005–2009 and 2010–2014).

Results
The most common reason for revision TKA was infection (29.3%), followed by aseptic loosening (19.7%), which together accounted for half of all revisions. Other indications for revision were instability (11.6%), osteolysis (10.4%), arthrofibrosis (8.1%), polyethylene (PE) wear (7.7%), malalignment/malposition (5.4%), patellar complication (3.1%), periprosthetic fracture (2.3%), pain (1.5%), and extensor mechanism deficiency (0.8%). Nearly half of early failures (47%) were due to infection. Osteolysis and PE wear made of a significantly higher proportion of revisions of TKAs performed prior to 2000 compared to index TKAs performed after 2000.

Conclusion
At our institution, infection was the most common reason for revision TKA. Infection had a higher rate of early revisions. Proportion of TKAs revised for osteolysis and PE wear was higher for TKAs performed prior to 2000. Proportion of revision TKA for infection and instability were higher with TKAs performed after 2000.
Understanding the effects of a complex psychological intervention on symptoms of depression in Goa, India: findings from a causal mediation analysis
Authors: Seward Nadine , Vansteelandt Stijn , Moreno-Agostino Darío , Patel Vikram , Araya Ricardo 
Published: August 2022

BackgroundUnderstanding how and under what circumstances a highly effective psychological intervention, improved symptoms of depression is important to maximise its clinical effectiveness.AimsTo address this complexity, we estimate the indirect effects of potentially important mediators to improve symptoms of depression (measured with the Patient Health Questionnaire (PHQ-9)) in the Healthy Activity Program trial.MethodInterventional in(direct) effects were used to decompose the total effect of the intervention on PHQ-9 scores into the direct and indirect effects. The following indirect effects were considered: characteristics of sessions, represented by the number of sessions and homework completed; behavioural activation, according to an adapted version of the Behavioural Activation for Depression Scale – Short Form; and extra sessions offered to participants who did not respond to the intervention.ResultsOf the total effect of the intervention measured through the difference in PHQ-9 scores between treatment arms (mean difference: −2.1, bias-corrected 95% CI −3.2 to −1.5), 34% was mediated through improved levels of behavioural activation (mean difference: −0.7, bias-corrected 95% CI −1.2 to −0.4). There was no evidence to support the mediating role of characteristics of the sessions nor the extra sessions offered to participants who did not respond to the treatment.ConclusionsFindings from our robust mediation analyses confirmed the importance of targeting behavioural activation. Contrary to published literature, our findings suggest that neither the number of sessions nor proportion of homework completed improved outcomes. Moreover, in this context, alternative treatments other than extra sessions should be considered for patients who do not respond to the intervention.
Dynamic analysis and sliding mode control method of 5-DOF manipulator
Authors: Jiang Quansheng , Cai Kai , Ma Ming 
Published: August 2022

Background: The five degree of freedom (5-DOF) manipulator greatly improves the machining efficiency and accuracy because of its high flexibility. They see wide application in various automation fields. The dynamic analysis and modeling of manipulators is of great significance to improve the working accuracy of a manipulator. Methods: For the robot task of sheet metal bending, a 5-DOF manipulator based on sliding mode control strategy is designed in this paper. Firstly, the dynamics of the 5-DOF manipulator is analyzed and the dynamic equation is established. Secondly, based on the principle of sliding mode control, a proportional integral (PI) sliding mode control method for 5-DOF manipulator based on nominal model is proposed. Finally, the sliding mode control simulation experiment of 5-DOF manipulator is carried out to verify its stability. Results: The 5-DOF manipulator with PI sliding mode control has a good control effect by overcoming the influence of modeling error due to its strong robustness, and effectively realizes good control stability. Conclusions: The experimental results show that the 5-DOF manipulator has good response speed and stability. The results also suggest that the manipulator can be widely used in complex scenarios such as medical surgery or industrial production line with high safety requirements.
Anticancer activity of Caesalpinia sappan by downregulating mitochondrial genes in A549 lung cancer cell line
Authors: Widodo Nashi , Puspitarini Sapti , Widyananda Muhammad Hermawan , Alamsyah Adzral , Wicaksono Septian Tri , Masruri Masruri , Jatmiko Yoga Dwi 
Published: August 2022

Background: The standardization and mechanism of action of Caesalpinia sappan as an anticancer agent are still lacking. This study aimed to understand the mechanism of action of C,sappan extract as an anticancer agent. Methods: This study was conducted using the A549 lung cancer cell line to understand the mechanism of action of C. sappan extract as an anticancer agent. The cytotoxicity activity, cell cycle progression, apoptosis, protein-related apoptosis (i.e., BCL-2and BAX protein) assays, and RNA sequencing were performed level were measured. Moreover, the antioxidant activity, total flavonoids, and phenolics of C.sappan were also assessed. Results: C.sappan has strong antioxidant activity (22.14 ± 0.93 ppm) total flavonoid content of (529.3 ± 4.56 mgQE/g), and phenolics content of (923.37 ± 5 mgGAE/g). The C.sappan ethanol extract inhibited cancer cell growth and arrested at G0/G1 phase of cell cycle, inducing apoptosis by increasing BAX/BCL-2 protein ratio in A549 lung cancer cell line. Furthermore, results from RNA sequencing analysis showed that C.sappan ethanol extract caused downregulation of genes acting on mitochondrial function including adenosine triphosphate (ATP) production and respiration. Conclusions: This study demonstrated that C.sappan has the ability to inhibit cancer cell growth by inducing apoptosis and mitochondrial dysfunction in A549 cells.