bluuebunny/crossref_metadata_2025_split huggingface dataset:
Catalyst for change: Lessons learned from overcoming barriers to providing safe abortion care in Médecins Sans Frontières projects
Authors: Kumar Manisha , Schulte‐Hillen Catrin , De Plecker Eva , Van Haver Ann , Marques Sonia Guinovart , Daly Maura , Vochten Hilde , Merzaghi Lisa , de le Vingne Brice , Saint‐Sauveur Jean François 
Published: October 2022

AbstractContextDespite instituting a policy in 2004, Médecins Sans Frontières (MSF) continuously struggled to routinely provide safe abortion care (SAC). In 2016, the organization launched an initiative aimed at increasing availability of SAC in MSF projects and increasing understanding of abortion‐related dynamics in humanitarian settings.MethodologyFrom March 2017 to April 2018, MSF staff conducted support visits to 10 projects in a country in sub‐Saharan Africa. Each visit followed a systematic approach with six key components and related tools that were later shared with teams worldwide. Data regarding women seeking abortion services and related outcomes were collected and analyzed retrospectively.ResultsFrom Q1 2017 through Q4 2019, SAC provision increased significantly in all 10 projects, rising from three to 759 safe abortions per quarter. Teams received 3831 patients seeking SAC and provided 3640 first and second trimester abortions, over 99% via medication methods. The overall complication rate was 4.29% and 0.3% for severe, life‐threatening complications. No major security incidents were reported. MSF provision of SAC worldwide increased from 781 in 2016 (the year before this initiative began) to 21,546 in 2019.ConclusionImplementation of SAC in humanitarian settings—even those with significant legal restrictions—is possible and necessary. Both first and second trimester medication abortion can be safely and effectively provided through both home‐ and facility‐based models of care. Programmatic data provide valuable insights into abortion‐related dynamics which must shape operational decision‐making. Addressing internal barriers and providing direct field support were key to stimulating organizational cultural change.
Pengaruh Tingkat Kerapatan dan Kedewasaan Hutan Mangrove dalam Memerangkap Sedimen di Muara Sungai Langsa Kota Langsa
Authors: Erlangga Erlangga , Gusnita Helmi , Syahrial Syahrial , Akla Cut Meurah Nur , Imamshadiqin Imamshadiqin , Ezraneti Riri , Firdaus Rian 
Published: October 2022

In November 2021, a study on the effect of mangrove density and maturity level on sediment trapping in the Langsa River estuary, Langsa City, was conducted with the objective of determining the density, maturity, and rate of sediment deposition around the mangrove forest, as well as the relationship between mangrove density and maturity level and sediment deposition rate. Mangrove vegetation was collected using a 40 m line transect parallel to the river flow and a 10 x 10 m plot, while sediment samples were collected using a sediment trap. The study's findings indicated that mangrove density varied between 675.00 and 1541.67 ind/ha, that mangrove maturity was uneven, with trunk diameters ranging between 4.14 and 9.83 cm and basal areas ranging between 30.45 and 72.93 m2/ha. Meanwhile, sediment deposition rates ranged between 26.94 and 277.73 mg/cm2/day, with a strong correlation between density and maturity of mangrove vegetation (98% and 99%, respectively). Penelitian terhadap pengaruh tingkat kerapatan dan kedewasaan hutan mangrove dalam memerangkap sedimen di muara Sungai Langsa Kota Langsa dilakukan pada bulan November 2021 dengan tujuan untuk mengetahui tingkat kerapatan, kedewasaan dan laju pengendapan sedimen di sekitar hutan mangrovenya serta untuk mengetahui keterkaitan antara tingkat kerapatan dan kedewasaan hutan mangrove terhadap laju pengendapan sedimennya. Vegetasi mangrove dikumpulkan dengan menarik transek garis sepanjang 40 m yang sejajar dengan aliran sungai dan dibuat plot berukuran 10 x 10 m, sedangkan sampel sedimennya diambil menggunakan sediment trap. Hasil penelitian memperlihatkan bahwa kerapatan mangrovenya berkisar antara 675.00 – 1541.67 ind/ha, kedewasaan mangrovenya tidak merata dengan diameter batangnya berkisar antara 4.14 – 9.83 cm dan basal areanya berkisar antara 30.45 – 72.93 m2/ha. Sementara untuk laju pengendapan sedimennya berkisar antara 26.94 – 277.73 mg/cm2/hari, dimana keterkaitan antara kerapatan dan kedewasaan vegetasi mangrovenya memiliki hubungan yang kuat (masing-masing 98% dan 99%).
Komunitas Spons (Porifera) pada Ekosistem Terumbu Karang di Manokwari, Indonesia
Authors: Sayori Nelly , Tururaja Tresia S. , Kolibongso Duaitd 
Published: October 2022

Sponges are one of the most influential benthic organisms in coral reef ecosystems. Many studies on sponge communities have been carried out globally, from the tropics to the sub-tropics. However, in Indonesia, the sponge community has not been sufficiently observed, especially its diversity and interactions with habitats. Manokwari, a developing city north of the Bird's Head Seascape region, Papua has a lack of information on benthic communities and no reports of sponges. This study is to examine the sponge community (diversity and distribution) in coral reef ecosystem. This study found that sponge richness (species and morphology) was categorized as “low”, with only 11 species with 8 morphological forms. The most common species included Niphates erecta, Stylissa carteri, and Pseudoceratina purpurea, while the most common growth forms were massive and encrusting, accounting respectively for 27.3% and 18.2% of the total number of species. The highest diversity was found on the island of Kaki (5 species) with the island of Nusmapi having an uneven distribution of sponges. The results of our study found that there was no relationship between sponge diversity and morphology. This baseline information is essential for management of marine biodiversity hotspots in taking decisions for marine life conservation. Spons merupakan salah satu organisme bentik yang paling berpengaruh dalam ekosistem terumbu karang. Banyak penelitian tentang komunitas spons telah dilakukan secara global, dari daerah tropis hingga sub tropis. Namun di Indonesia, komunitas spons belum cukup diamati, terutama keanekaragaman dan interaksinya dengan habitat. Manokwari, kota berkembang di utara dari wilayah Bentang Laut Kepala Burung (BLKB), Papua memiliki kekurangan informasi tentang komunitas bentik dan tidak ada laporan tentang spons. Studi ini untuk mengkaji komunitas spons (keanekaragaman dan distribusi) pada ekosistem terumbu karang. Penelitian ini menemukan kekayaan spons (spesies dan morfologi) yang dikategorikan “rendah”, dengan hanya 11 spesies dengan 8 bentuk morfologi. Spesies yang paling umum termasuk Niphates erecta, Stylissa carteri, dan Pseudoceratina purpurea, sedangkan bentuk pertumbuhan yang paling umum adalah massif dan encrusting dengan menyumbang masing-masing 27,3% dan18,2% dari total jumlah spesies. Keanekaragaman tertinggi ditemukan di pulau Kaki (5 spesies) dengan pulau Nusmapi memiliki sebaran spons tidak merata. Hasil penelitian kami menemukan tidak ada hubungan antara keanekaragaman spons dengan bentuk morfologi. Informasi dasar ini sangat penting untuk pengelolaan hotspot keanekaragaman hayati dalam perumusan keputusan untuk konservasi biota laut.
Aktivitas Antijamur dari Bakteri Sedimen Mangrove Terhadap Candida albicans dan Malassezia furfur
Authors: Setyati Wilis Ari , Arifidyani Ananda , Susanto AB 
Published: October 2022

Candida albicans and Malassezia furfur are fungal pathogens that can cause skin infections. This disease can be easily transmitted from one individual to another. The use of antibiotic drugs can cause pathogenic fungi to become resistant. Therefore, there is a need for the development of new antifungal substances, one of which comes from mangrove sediment bacteria. This research was conducted with the aim of obtaining bacterial isolats from mangrove sediments that have the ability to fight the fungal pathogens Candida albicans and Malassezia furfur. The method in this research is descriptive exploratory laboratory which includes isolation sampling and isolat purification, morphological characterization, screening, antifungal activity test and biochemical test. The results of this study were obtained 3 bacterial isolats that have antifungal activity against the fungal pathogens Candida albicans and Malassezia furfur. The results of the identification of biochemical tests showed that the isolat S.ISP2 was identified as the genus Pseudomonas; isolat S.ISP4 identified as Genus Moraxella; and isolat KJ.MA1 identified as Genus Vibrio Candida albicans dan Malassezia furfur merupakan patogen jamur yang dapat menyebabkan penyakit infeksi kulit. Penyakit ini dapat dengan mudah ditularkan dari satu individu ke individu lainnya. Penggunaan obat antibiotik dapat mengakibatkan jamur patogen menjadi resisten. Oleh karena itum eprlunya ada pengembangan zat antijamur baru yang salah satunya berasal dari bakteri sedimen mangrove. Penelitian ini dilakukan dengan tujuan untuk mendapatkan bakteri isolat dari sedimen mangrove yang memiliki kemampuan untuk melawan jamur patogen Candida albicans dan Malassezia furfur. Metode pada penelitian ini adalah deskriptif eksploratif laboratory yang meliputi sampling isolasi dan purifikasi isolat, karakterisasi morfologi, skrining, uji aktivitas antijamur dan uji biokimia. Hasil dari penelitian ini adalah didapatkan 3 bakteri isolat yang memiliki aktivitas antijamur terhadap jamur patogen Candida albicans dan Malassezia furfur. Hasil identifikasi uji biokimia menunjukkan bahwa isolat S.ISP2 teridentifikasi sebagai Genus Pseudomonas; isolat S.ISP4 teridentifikasi sebagai Genus Moraxella; dan isolat KJ.MA1 teridentifikasi sebagai Genus Vibrio.
The influence of proteoforms: assessing the accuracy of total vitamin D-binding protein quantification by proteolysis and LC-MS/MS
Authors: Kilpatrick Lisa E. , Bouillon Roger , Davis W. Clay , Henderson Clark M. , Hoofnagle Andrew N. , Pauwels Steven , Vanderschueren Dirk , Waelkens Etienne , Wildiers Hans , Yen James H. , Phinney Karen W. 
Published: October 2022

Abstract

Objectives
Vitamin D-binding protein (VDBP), a serum transport protein for 25-hydroxyvitamin D [25(OH)D], has three common proteoforms which have co-localized amino acid variations and glycosylation. A monoclonal immunoassay was found to differentially detect VDBP proteoforms and methods using liquid chromatography-tandem mass spectrometry (LC-MS/MS) might be able to overcome this limitation. Previously developed multiple reaction monitoring LC-MS/MS methods for total VDBP quantification represent an opportunity to probe the potential effects of proteoforms on proteolysis, instrument response and quantification accuracy.

Methods
VDBP was purified from homozygous human donors and quantified using proteolysis or acid hydrolysis and LC-MS/MS. An interlaboratory comparison was performed using pooled human plasma [Standard Reference Material® 1950 (SRM 1950) Metabolites in Frozen Human Plasma] and analyses with different LC-MS/MS methods in two laboratories.

Results
Several shared peptides from purified proteoforms were found to give reproducible concentrations [≤2.7% coefficient of variation (CV)] and linear instrument responses (R2≥0.9971) when added to human serum. Total VDBP concentrations from proteolysis or amino acid analysis (AAA) of purified proteoforms had ≤1.92% CV. SRM 1950, containing multiple proteoforms, quantified in two laboratories resulted in total VDBP concentrations with 7.05% CV.

Conclusions
VDBP proteoforms were not found to cause bias during quantification by LC-MS/MS, thus demonstrating that a family of proteins can be accurately quantified using shared peptides. A reference value was assigned for total VDBP in SRM 1950, which may be used to standardize methods and improve the accuracy of VDBP quantification in research and clinical samples.
The new, race-free, Chronic Kidney Disease Epidemiology Consortium (CKD-EPI) equation to estimate glomerular filtration rate: is it applicable in Europe? A position statement by the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)
Authors: Delanaye Pierre , Schaeffner Elke , Cozzolino Mario , Langlois Michel , Plebani Mario , Ozben Tomris , Cavalier Etienne 
Published: October 2022

Abstract
The EFLM recommends not to implement the race-free Chronic Kidney Disease Epidemiology Consortium (CKD-EPI) equation in European laboratories and to keep the 2009 version of the CKD-EPI equation, without applying a race correction factor. This recommendation is completely in line with a recent Editorial published by the European Renal Association who has also proposed to change to a novel equation only when it has considerably better performance, trying to reach global consensus before implementing such a new glomerular filtration rate (GFR) estimation equation. In Europe, this equation could be for instance the new European Kidney Function Consortium (EKFC) equation, which is population-specific, developed from European cohorts and accurate from infants to the older old. Beyond serum creatinine, the estimating equations based on cystatin C will probably gain in popularity, especially because cystatin C seems independent of race. Finally, we must keep in mind that all GFR equations remain an estimation of GFR, especially rough at the individual level. Measuring GFR with a reference method, such as iohexol clearance, remains indicated in specific patients and/or specific situations, and here also, the role of the clinical laboratories is central and should still evolve positively in the future.
El espacio Atlántico/Caribe guatemalteco: una construcción histórica y territorial liberal 1871-1959
Authors: Véliz Catalán Néstor 
Published: October 2022

La motivación central de este artículo reside en explicar la forma en que se construyó la representación del territorio caribeño de Guatemala desde 1871. Esta orientación conlleva a cuestionar la forma en que la oficialidad denominó este espacio, lo que implica inquirir sobre el sentido discursivo, ideológico y político de nombrarlo como “Atlántico”. Ello remite a revisar las tendencias de los movimientos de asentamiento urbano y construcción de vías de comunicación desde la época colonial. Subyace la tesis de que, la denominación antes expresada, obedece a una certeza de parte de los intelectuales liberales de llamar el espacio caribeño de la forma citada al considerar que las aguas que lo bañan constituyen un espacio común con Europa occidental, paradigma civilizatorio. La reproducción de dicha forma de nombrar el espacio tiene lugar con la pervivencia de un discurso excluyente que llevó a asumir la región como “ventana” hacia Europa, obviando el contexto inmediato.
Mentha: A review on its bioactive compounds and potential health benefits
Authors: Wani Sajad Ahmad , Naik Haroon , Wagay Javeed , Ganie Nawaz Ahmad , Mulla Mehrajfatema Z. , Dar B.N. 
Published: October 2022

Mint (Mentha) is a medicinal herb, which possesses a lot of bioactive components. Globally, it has been used as a flavor enhancer in foods. Due to the presence of phenolic acids and flavonoids, it is considered to have a greater number of antioxidants. Mint has been linked to physiological benefits to humans that include protection against microbes; anticancer and antiallergenic properties; positive effects in reducing blood sugar; analgesic property; cures loose motion, indigestion, gas, and irritation bowel syndrome; gives relief from respiratory problems; has wound healing activity; and is good for breastfeeding. The present paper reviews the evidence-based research regarding the bioactive components and health benefits of the mint plant.
Significance of metabolomic disorders in infertility and miscarriage in patients with endometriosis
Authors: Ermolova Natalya V. , Levkovich Marina A. , Petrov Yury A. , Avrutskaya Valeria V. , Krukier Irina I. , Savchenko Anastasia V. 
Published: October 2022

BACKGROUND: Despite numerous studies, the causal relationships of endometriosis, associated infertility and miscarriage have not yet been clarified.
AIM: This article discusses the significance of insulin-like growth factor 1, matrix metalloproteinase 9 and activin A levels in the blood serum and peritoneal fluid of patients of reproductive age with endometriosis in the clinical aspects of infertility and miscarriage and as per stages of the disease.
MATERIALS AND METHODS: The overall group consisted of patients with endometriosis (group I: stages III, n = 25; group II: stages IIIIV, n = 38). The control group comprised patients without the disease (group III: n = 25). The stage of endometriosis was classified during laparoscopy in accordance with revised American Society for Reproductive Medicine classification (1996).
RESULTS: In endometriosis, primary infertility predominated (up to 40.8% of cases), while secondary infertility occured in 21.2% of cases. Primary infertility accounted for 68.0% of cases in stages III versus 39.5% of cases in stages IIIIV. Secondary infertility accounted for 28.0% versus 23.0% of cases, respectively. In minimal stages of the disease, non-developing pregnancies (12.0%) and spontaneous miscarriages (4.0%) occurred in 16.0% of cases. In severe stages, these nosologies occurred in an equal percentage of cases (5.2%).
The group of patients with endometriosis showed multidirectional changes in activin A levels in the blood serum and peritoneal fluid, a decrease in matrix metalloproteinase 9 level by 27% in the peritoneal fluid and an increase in insulin-like growth factor 1 level by 36% in the blood serum. In minimal stages of endometriosis, the tendencies of changes in the parameters remained intact as compared to the overall group, the correspondence of matrix metalloproteinase 9 levels to the control values in the blood serum and peritoneal fluid being noteworthy. Stages IIIIV of the disease implied more significant changes as compared to the control or the overall group of patients. We found a decrease in insulin-like growth factor 1 level by 56% in the peritoneal fluid, a decrease in activin A level by 19% in the blood serum with an increase in the parameter by 27% in the peritoneal fluid, and a decrease in matrix metalloproteinase 9 level by 4% in the peritoneal fluid.
CONCLUSIONS: Modifications of the studied compounds activin A, MPP-9 and IPFR-1 are associated with infertility and miscarriage in patients with endometriosis.
Russia and Japan as an Image of the “Other” on the Pages of British Periodicals of the Early 60s of the 19th Century
Authors: Gella Tamara 
Published: October 2022

The article is devoted to the analysis of the socio-cultural perception of Russia and Japan as an image of the “other” in British society in the early 60s of the 19th century. Unlike previous studies of this problem, the source base was the publications of a number of British periodical journals. The choice of Russia and Japan as objects of study is due to the fact that both countries were perceived by English contemporaries as Asian countries with an Asian mentality of their population. However, Russia was also perceived as a Slavic country. In this regard, conducting a fragmentary cross-section of country studies within the framework of the British socio-cultural perception of Japan and Russia may be of interest both for understanding the relationship of Great Britain with these states, and for clarifying the Middle Victorian mentality as such. The article emphasizes that the criteria for the authors’ coverage of the events in Japan and Russia and the life of their peoples were different. With regard to Japan, the articles described not only its state system and the political situation in the country, but considerable attention was paid to Japanese nature, architecture, commerce, everyday life and culture of the Japanese. As for the Russian subjects, the materials were mainly devoted to the domestic and foreign policy of Russia since the beginning of the reign of Alexander II with a constant excursion into the history of the country. In this article, conclusions were drawn that British magazines, creating “Russian” and “Japanese” images, proceeded from the presence of the so-called “Japanese myth”, on the one hand, and on the other hand, from the prevailing stereotypical perception of the Russian people, thereby distorting the real picture of Russia and Japan in the early 60s of the 19th century.
Determining the Relationship Between EEG Findings and Prognosis of Patients with Decreased Consciousness Following Head Injury
Authors: Mirzaasgari Zahra , Zamani Babak , Vahedifard Farzan , Kheirkhah Parnian , Homayooni Amirsina 
Published: October 2022

Background: Head injury is a silent epidemic in developed countries and is one of the leading causes of death and disability. Determining the precise and early prognosis of head injury, especially in severe cases, is essential and will help define treatment actions and rehabilitation plans and counsel other family members. The purpose of this study was to determine the relationship between electroencephalographic findings and prognosis in patients with decreased levels of consciousness due to head injury.
Methods: This is a retrospective descriptive-analytical study on patients with reduced levels of consciousness due to head injury, referred to Tir Hospital (Tehran) in the first half of 2018. Overall, 52 patients were studied and divided according to their GCS into two groups: favorable prognosis (30 patients, 57.7%) and poor prognosis (22 patients, 42.3%). We tried to find the relation between EEG findings such as voltage, electrographic seizures, periodic and rhythmic patterns, and the prognosis.
Results: Normal voltage was associated with a favorable prognosis, with a high specificity (95%) and a less robust sensitivity (63%). The isoelectric voltage (electrocerebral shutdown) was associated with a poor prognosis. Normal and isoelectric voltages were good predictors with 95% and 100% Positive Predictive Values (PPV), respectively. There was a statistically significant relationship between EEG frequency range and prognosis.
Conclusion: This study showed that EEG parameters might provide valuable data regarding the prognosis of decreased consciousness of patients with a head injury, also for the guidance of physicians and informing the patients’ family.
The Association Between Depression, Impulsive Behaviors and Suicidal Ideation with Serum Low Density Lipoprotein (LDL) Level in Patients with Morbid Obesity: A Cross-Sectional Study
Authors: Jolfaei Atefeh Ghanbari , Soheilipour Fahimeh , Mohammadnia Fateme , Esmaeeli Soode Tajik 
Published: October 2022

The aim of this study was to compare the severity of depression, impulsive behaviors and suicidal ideation in patients with obesity with Low Density Lipoprotein (LDL) below 100 or above 100 mg/dl.
Methods: This was a cross-sectional study. The study population included patients with obesity referred to the obesity clinic of Rasool-e-Akram Hospital affiliated to Iran University of Medical Sciences, Tehran, Iran. Patients were divided into two groups with LDL below and above 100 mg/dl. The Beck scale for suicidal ideation and Depression and the Barratt Impulsiveness Scale questionnaires were filled. Finally, the score of questionnaires and demographic variables were compared between the two groups. P-values below 0.05 were considered as statistically significant.
Results: 300 patients entered the study. There was a statistically significant difference regarding the severity of depression betweeen the two groups, as in patients with LDL over 100 mg/dl, depression was more severe (p-value = 0.02). Moreover, there was a statistically significant difference regarding the impulsivity score and its subgroups or the suicidal ideation between the two groups (higher in patients with LDL) above 100 mg/dl, p-values <0.05).
Conclusion: Elevated serum LDL levels could be associated with psychological factors such as depression or suicidal ideation and motor, cognition and unplanned impulsivity in patients with morbid obesity. This might be justified by an increase in BMI. Further clinical trials are required to assess these findings.