bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myocardial injury and inflammatory response in percutaneous device closures of pediatric patent ductus arteriosus Running Title: Myocardial and inflammatory response of patent ductus arteriosus
Authors: Luo Zeng-Rong , Yu Ling-Li , Zheng Guo-Zhong , Huang Zhong-Yao 
Published: March 2022

Abstract
Background
The percutaneous device closure of patent ductus arteriosus (PDA) is widely used in clinical practice, however full data on the changes in myocardial injury and systemic inflammatory markers’ levels after PDA in children are not fully reported.
Methods
We have conducted a retrospective analysis of the medical records of 385 pediatric patients in our hospital from January 2017 to December 2019. The patients were distributed into five groups. The first four (A, B, C and D) included patients divided by the type of the surgical closure methods, namely ligation, clamping, ligation-combined suturing and ligation-combined clamping, respectively. The fifth group E comprised of percutaneous device PDA patients. All recorded medical and trial data from the five groups were statistically studied.
Results
No serious complications in the patients regardless of the classification group were reported. Our results suggested that there were no considerable differences between the groups at the baseline (P > 0.05). Group E demonstrated a significantly smaller operative time and length of hospital stay (LOS), less intraoperative blood loss while on the other hand, a higher total hospital cost than the other four groups (P < 0.001). Interestingly, the comparison of the inflammatory factors such as white blood cells (WBC) count, C-reactive protein (CRP), procalcitonin (PCT) and interleukin-6 (IL-6), as well as the myocardial injury markers (CK-MB and troponin I) did not show a significant increase ( P > 0.05) among the four groups. On the contrary, when the aforementioned factors and markers of all the surgical groups were compared to those in group E, we observed significantly higher speed and magnitude of changes in group E than those in groups A, B, C, and D (P < 0.001).
Conclusion
Although the percutaneous device closure of PDA is more comforting and drives fast recuperation in comparison to conventional surgery, it provokes myocardial injury and overall inflammation. Group E in our studies showcased a significant increase in the measured pro-inflammatory and myocardial injury blood markers.
Untangling the Genetic Control of Maize Plant Aarchitecture Plasticity
Authors: Incognito Salvador Juan Pablo , Maddonni Gustavo , López César Gabriel 
Published: March 2022

Abstract
Phenotypic plasticity (PP) is the ability of an organism to produce multiple phenotypes in response to environmental changes. In cultivated species, such as maize (Zea mays L.), the PP of plant architecture traits will play an important role in the adaptation of genotypes to unpredictable scenarios given by climate change, marginal areas, and seeding with variable plant density (D). We bring information to improve the understanding of the environmental modulation of PPs of plant architecture traits of maize, untangling their genetic bases, and testing the hypothesis of independent genetic control of the traits per se and their PPs. The PP of traits related to leaf area, spatial distribution of leaf area and stem architecture [(leaf area (LA), maximum leaf width (LW), maximum leaf length (LL), leaf orientation value (LOV), vertical leaf angle (VA), leaf length to the flagging point (LF), LE/LL relationship (LFLL), azimuthal leaf orientation (AZ), ear height (EH), plant eight (PH), EH/PH relatioship (EHPH) and stem diameter (SD)] were estimated using 160 RILs from the IBM B73 × Mo17 Syn4 population, cultivated under two contrasting D (5 and 10 pl m− 2) during two growing seasons that determined different environmental conditions. Data were phenotypically analyzed and quantitative traits loci (QTLs) were mapped. For leaf area and stem architecture related traits, high mean values of traits per se were related with high PPs values at low intraspecific competition while low mean values were observed at high intraspecific competition. The opposite response was found on leaf orientation related traits, with the exception of AZ. Forty-eight QTLs were detected for PP of plant architecture related traits on all chromosomes with exception of chromosome 7. There was no phenotypic correlation and no co-located QTLs for traits per se and their PPs. This independent genetic control for traits per se and their PPs would allow breeders to develop genotypes adapted to specific environments selecting for high or low PP in combination with high or low values for relevant agronomic traits.
Distribution of Amphibians and Reptiles in Agricultural Landscape Across Europe
Authors: Bancila Raluca , Lattuada Matteo , Sillero Neftalí 
Published: March 2022

Abstract
ContextTo understand the herpetofauna decline in agricultural landscapes with herpetofauna presence and evaluating the species-specific and species richness patterns in response to their features.ObjectivesThis work aimed to explore how different crop categories (i.e. agroforestry, irrigated, dry and woody crops and pastures), crop extent and heterogeneity affect herpetofauna distribution and diversity patterns at two different spatial resolutions, UTM 50km2 and UTM 10km2.MethodsWe documented the occurrence of European amphibians and reptiles in crops and quantify crop extent and heterogeneity in 50km2 and 10km2 grid cells. ResultsWe recorded 72 amphibian and 142 reptile species at UTM 50 km2 and UTM 10 km2 grids. The geographic location of peaks and troughs of crop extent and of species richness at UTM 50km2 did not coincide with those at UTM 10km2. Our results showed that amphibian and reptile species presence and richness are influenced by crop category, extent and heterogeneity and that spatial patterns were scale dependent. Species richness of both amphibians and reptiles was generally negatively correlated with crop extent but was enhanced by crop heterogeneity.ConclusionsOur results provide useful information for future risk assessment studies of herpetofauna, such as to identify candidates (i.e. “representative species”) for pesticide effect field studies or for studies of risk refinement though radio-tracking.
Tracking the 3D atomic structures during thermal treatment and catalytic activity of individual Pt nanoparticles
Authors: Chun Hoje , Kang Joonhee , Kang Dohun , Hong Sung Jun , Heo Junyoung , Cho Hyeongjin , Heo Jaehyeon , Kim Byung Hyo , Park Jungwon , Han Byungchan 
Published: March 2022

Abstract
To acquire a crystal-clear structure-performance relation for heterogeneous nanocatalysts, precise tracking of the structural evolution from the precursor to final product in the real time and space domain is important but also extremely challenging. In archetype preparation, thermal treatment is a crucial step for activation. Especially, for colloidally synthesized nanoparticles, the ligand detachment process to expose surfaces is completed with thermal treatment. Here, we track the three-dimensional atomic structural change of ligand-protected platinum nanoparticles under ligand detachment and thermal annealing processes. The structural transformation with time is shown by experimental decoupling and tracking of each atomic coordinate through molecular dynamics simulations with machine-learning potentials. Acquisition of structural data is integrated with a computational catalysis framework to identify a structure-activity correlation in the CO oxidation reaction. We find that colloidally synthesized platinum nanoparticles create unique atomic tensile strain and that varying the particle size tunes the ensemble effect of active sites.
Hematopoietic stem cell transplantation for acute lymphoblastic leukemia: why do adolescents and young adults outcomes differ from those of children? A retrospective study on behalf of the Francophone Society of Stem Cell Transplantation and Cellular Therapy (SFGM-TC).
Authors: Grain Audrey , Rialland Fanny , Chevallier Patrice , Blin Nicolas , Dalle Jean-Hugues , Michel Gérard , Dhédin Nathalie , de Latour Régis Peffault , Pochon Cécile , Yakoub-Agha Ibrahim , Bertrand Yves , Sirvent Anne , Jubert Charlotte , Forcade Edouard , Berceanu Ana , Gandemer Virginie , Schneider Pascale , Bay Jacques-Olivier , Rohrlich Pierre-Simon , Brissot Eolia , Paillard Catherine , Plantaz Dominique , Quoc Stéphanie Nguyen , Gonzales Fanny , Maillard Natacha , Planche Lucie , Baruchel Andre 
Published: March 2022

Abstract
Purpose: In the Acute Lymphoblastic Leukemia (ALL) landscape, Adolescents and Young Adults (AYA) often present high-risk diseases and increased chemotherapy-related toxicity. Studies analyzing outcomes of AYA after Hematopoietic Stem Cell Transplant (HSCT) are scarce. Our study aimed to compare the outcomes of children and AYA with ALL after HSCT and to determine factors influencing potential differences. Method: 891 patients, from the SFGM-TC registry, aged between 1 and 25 years who received HSCT between 2005 and 2012 were included. Outcomes of AYA were compared to the ones of their younger counterparts.Results: Five-year OS and GRFS were lower in AYA: 53.1% versus 64% and 36% versus 47% (p = 0.0012 and p=0.007 respectively). While CIR were similar in both groups, 5 year-Treatment Related Mortality was higher in AYA: 19% versus 13% (p=0.04). The lower GRFS in AYA was mainly explained by a higher chronic Graft versus Host Disease (cGvHD) incidence: 32% versus 19% (p<0.001). Use of Peripheral Blood Stem Cells and use of anti-thymoglobulin appeared to be the main factors impacting cGvHD occurrence in AYA. Conclusion: AYA have worse outcomes than children after HSCT for ALL because of a greater risk of TRM due to cGvHD. HSCT practices should be questioned in this population.
Evaluation of Dental Radiography and Treatment Needs in 3-19-year-old Swedish Patients: a Retrospective, Controlled Comparison of Extremely Preterm–born and Very Preterm–born With Full Term–born Children and Adolescents
Authors: Vicente António , Alward Lubna , Wiedel Anna-Paulina , Becker Magnus , Shi Xie-Qi , Hellén-Halme Kristina , Brogårdh-Roth Susanne 
Published: March 2022

Abstract
Background: Preterm birth has been shown to cause various long-term health issues. Children who were born preterm have also been observed to have more behaviour management problems (BMP) during dental examinations and treatment than children born full term. It is known that dental radiographic examinations can be uncomfortable and cause anxiety in paediatric patients. Our aims were to compare a matched control group of full term-born children and adolescents with preterm–born children and adolescents concerning oral health outcomes, such as prevalence of BMP, missed appointments, caries, treatment needs, including the need for a dental radiographic examination, and experience of orthodontic treatment.Methods: The present study included 311 patient files: 122 very preterm–born and 33 extremely preterm–born children and adolescents (<32 gestational week). A matched control group of 156 full term–born children and adolescents (≥ 37 gestational week) was analysed for comparison. Various factors, including BMP, missed appointments, dental caries, and radiographic examinations, were retrieved from the dental records for three age intervals: 3–6 years, 7–12 years, and 13–19 years.Results: Extremely preterm–born children missed significantly more dental appointments and presented with more BMP during dental examinations and treatment than full term–born children in the 3–6-year age group; the same was observed for the very preterm–born in the 7–12-year age groups. No significant differences in BMP during bitewing and periapical examinations or in number of periapical and panoramic radiographs occurred between the groups in any age interval. Conclusions: The same dental care approach can be used for any child, regardless of gestational age at birth, regarding dental radiography, caries, and treatment needs. However, preterm–born children and adolescents should be given more flexibility in rebooking and receive reminders for scheduled visits with the general dental team.
A Novel Prognostic Signature for Hepatocellular Carcinoma Based on Sumoylation-Related Genes
Authors: Wang Jianping , Cong Peipei , Jin Zhipeng , Liu Lingli , Sun Dongxu , Zhu Wenjing , Shi Guangjun 
Published: March 2022

Abstract
SUMOylation (SUMO modification) has been confirmed to play an important role in the progression of various malignancies. As the value of SUMOylation-related genes (SRGs) in prognosis prediction of hepatocellular carcinoma (HCC) has not been explored, we aimed to construct a HCC SRGs signature. RNA sequencing was utilized to identify differentially expressed SRGs. The 87 identified genes were used in Univariate Cox regression analysis and the Least Absolute Shrinkage and Selection Operator (LASSO) analysis to build a signature. The signature was prognostic in the TCGA and ICGC datasets. The GSEA revealed that the risk score was also associated with common cancer-related pathways. The ssGSEA showed NK cells in the high-risk group was significantly reduced. The sensitivities of anti-cancer drugs confirmed the sensitivity of the high-risk group to sorafenib was lower. Further, Our cohort showed that risk scores were correlated with advanced grade and Vascular invasion (VI). Finally, the results of H&E staining and immunohistochemistry of Ki67 showed that higher-risk patients are more malignant.
A potential involvement of PCSK9 in autism etiology: sequence variations, protein concentration and promoter methylation
Authors: Salem Sohair , Mosaad Rehab M. , Lotfy Randa S. , Ashaat Engy A. , Ismail Samira 
Published: March 2022

Abstract
Beside its main role in controlling blood cholesterol, PCSK9 has a role in regulating neuronal development and apoptosis. Therefore, we suggested, for the first time, a possible involvement of PCSK9 as susceptibility gene for autism. This case-control study used sanger sequencing to analyze sequence variations in the twelve exons of PCSK9 gene and their flanking intronic sequences. ELISA assay was used to determine plasma PCSK9 concentration. Methylation percentage of PCSK9 promoter-associated CpG island was assessed by methylation specific PCR(MSP). Forty-three variants were found; out of them, seven variants were differentially- existed between autistic cases and controls. rs45448095, rs45613943, rs630431, rs529500286 and rs45439391 are considered as risk factors for autism, while rs11800231 and rs483462 are protective variants. Plasma level of PCSK9 concentration is significantly elevated and promoter methylation percentage is significantly reduced in autistic cases than control subjects (P = 0.000 and 0.002, respectively). Roc curve analysis identified area under curve (AUC) for plasma PCSK9 concentration as 0.915 and for promoter methylation percentage as 0.693. Additionally, two novel variants (g.23809C > T in intron 11 and g.24071T > G in 3 ̀ UTR) were identified. This is the first study to correlate PCSK9 and autism and concluded a possible involvement of PCSK9 in autism pathogenesis as a new susceptibility gene and recommend further studies with a greater number of subjects.
Identification of NEK7 Inhibitors: Structure based Virtual Screening, Molecular Docking, Density functional theory calculations and Molecular Dynamics Simulations
Authors: Aziz Mubashir , Ejaz Syeda Abida , Rehman Hafiz Muzzammel , Al-Buriahi M. S. , Siddique Farhan , Somaily H. H. , Alrowaili Z. A. 
Published: March 2022

Abstract
NEK7 plays a crucial role in many signaling pathways and contributes to a variety of cancers. Therefore, NEK7 has long been considered an attractive drug target in anti-cancer drug discovery. However, only a few efforts have been made in development of NEK7 inhibitors with selectivity. In the present study, we have investigated FDA approved kinase inhibitors as potential NEK7 inhibitors. Although more than 200 FDA approved drugs are available but none is known to inhibit NEK7 protein. These findings motivated us to design in-silico approach for investigation and identification of NEK7 protein. In the current study, Structure-based virtual screening and molecular docking were carried out to identify potential NEK7 inhibitors. Dacomitinib and Neratinib was selected depending upon their potential activities against various cancer cell lines. These drugs were subjected to density functional theory calculations which demonstrated the chemical reactivity of both drugs. Furthermore, molecular docking studies were conducted using Molecular Operating environment 2015.10 and conformations with high docking scores and strong interactions were selected for further analysis. Absorption, distribution, metabolism, elimination and toxicity (ADMET) profile evaluation was also carried out to ascertain toxicity profile of both drugs. The proposed inhibitor–protein complexes were further subjected to Molecular Dynamics (MD) Simulations studies involving root-mean-square deviation and root-mean-square fluctuation to explore the binding mode stability inside active pockets. Finally, both drugs were obtained as potential inhibitors of NEK7 protein. All these analyses provide a reference for the further development of NEk7 inhibitors.
Neural Mechanisms Underlying Empathy During Alcohol Abstinence: Evidence from Connectome-Based Modeling
Authors: Yao Guanzhong , Wei Luqing , Jiang Ting , Dong Hui , Baeken Chris , Wu Guorong 
Published: March 2022

Abstract
Empathy impairments have been linked to alcohol dependency, even during abstinent periods. Nonetheless, the neural underpinnings of abstinence-induced deficits in empathy remain unclear. In this study, to predict the empathy capability of abstinent alcoholics (n = 47) versus healthy controls (n = 59), we employed connectome-based predictive modeling (CPM) by using whole brain resting-state functional connectivity (rs-FC). In addition, the generalizability of the predictive model (i.e., one group treated as a training dataset and another one treated as a test dataset) was performed to determine whether healthy controls and abstinent alcoholics share common neural fingerprints underlying empathy. Although no comparable predictive models were observed in the abstinence group, our results showed that individual empathy scores in the healthy group can be reliably predicted by functional connectivity from the default mode network (DMN) to the sensorimotor network (SMN), the occipital network, and the cingulo-opercular network (CON). Furthermore, the identified connectivity fingerprints of healthy controls could be generalized to predict empathy in the abstinence group. These findings indicate that neural circuits accounting for empathy may be disrupted by alcohol use and the impaired degree varies greatly among alcohol abstinent individuals.
Serum exosomal miR-1258: A novel biomarker for the diagnosis of acute exacerbations of COPD
Authors: Wang Fei , Yang Boxin , Qiao Jiao , Bai Linlu , Li Zijing , Sun Wenyuan , Liu Qi , Yang Shuo , Cui Liyan 
Published: March 2022

Abstract
BACKGROUNDAcute exacerbation chronic obstructive pulmonary disease (AECOPD) has a high mortality rate, rapid identification of AECOPD from COPD is critical for timely treatment and improved prognosis. However, there is no efficiency biomarker for diagnosing AECOPD. OBJECTIVEThe purpose of this study was to find biomarkers that can quickly and accurately diagnose AECOPD and establish an effective laboratory diagnostic strategy.METHODS45 normal controls (NC), 42 patients with stable COPD (SCOPD), and 66 patients with AECOPD were enrolled in our study. Exosomes were isolated by ultracentrifuge and verified by morphology and specific biomarkers. Fluorescent quantitation polymerase chain reaction (qRT-PCR) was used to detect the expression of micro RNAs (miRNAs) in serum and exosomes. We constructed the diagnostic models of AECOPD using logistic regression and machine learning methods. RESULTSThe levels of miR-1258 in the patients with AECOPD were higher than that in the patients with COPD and NC both in serum and exosomes. The ability of exosomal miR-1258 (AUC=0.851) to identify AECOPD from SCOPD was superior to neutrophil to lymphocyte ratio (NLR) (AUC=0.797), serum miR-1258 (AUC=0.790), neutrophil count (AUC=0.727), white blood cell count (WBC) (AUC=0.654) and C-reactive protein (CRP) (AUC=0.635), and the combination of exosomal miR-1258 and NLR can increase the AUC to 0.944, with a sensitivity of 81.82%, and specificity of 97.62%. The cross-validation of the models displayed that the logistic regression model based on exosomal miR-1258, NLR and neutrophil count had the best accuracy (0.880) in diagnosing AECOPD from SCOPD. Training set for decision tree showed that of the 108 patients with SCOPD and AECOPD, 50 patients with miR-1258 greater than 0.197, 49 of them had a neutrophil greater than 2.705x10 9 /L were diagnosed with AECOPD. Serum exosome miR-1258 was positively correlated with neutrophil count (r=0.57, p<0.001), WBC (r=0.50, p<0.001), CRP (r=0.33, p<0.001), lactic dehydrogenase (LDH) (r=0.29, p<0.001), and NLR (r=0.24, p<0.05).CONCLUSIONSerum exosomal miR-1258 is associated with inflammation, and can be used as a valuable and reliable biomarker for the diagnosis of AECOPD, and the establishment of diagnostic model based on miR-1258, NLR and neutrophils can help improve the accuracy of AECOPD diagnosis. Serum exosomal miR-1258 for AECOPD
Prioritizing patients for medication review by acute ward pharmacists – a mixed methods study in a Danish hospital.
Authors: Almarsdóttir Anna Birna , Haq Romana , Nørgaard Josefine Dalgaard Scheel Vandel 
Published: March 2022

Abstract
Background Little is known about how pharmacists think and act in the situation when they need to prioritize patients without prioritization tools. Aim To understand the individual pharmacist professional’s analytic processes when prioritizing patients for medication review in an emergency department.Method A mixed-methods study with pharmacists working in one emergency department in Denmark. Qualitative participant observations of all seven pharmacists who work at the emergency department were carried out. Semi-structured interviews with six of the pharmacists were held closely in time to the observations. Observations for each pharmacists’ patients were analysed inductively looking for patterns and themes. The interviews were audiotaped, transcribed verbatim and analysed inductively seeking to understand pharmacists’ analytic processes when prioritizing patients for medication review. Results Observations showed that non-clinical prioritization rules had been implemented according to the wishes of the ward management, which affected how the pharmacists prioritized patients. The ways pharmacists prioritized was more targeted with more clinical experience. In the interviews, pharmacists told how they used the rules set out by ward management, how their collaboration with physicians was vital to prioritization, and how the pharmacy profession could better contribute to the ward team. Conclusion Although the ward-established rules increased efficiency, it was not deemed the best way to detect the clinically meaningful patients for the pharmacists working at the Emergency Department. A prioritization tool and sitting closer to the physicians at the ward would help pharmacists be further integrated into patient care and improve prioritization of patients for medication reviews.