bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative genomic and transcriptomic analyses reveal potential gene regulatory network associated with berry size and early- ripening caused by bud sport in grapes
Authors: Huang Jianquan , zhang guan , Li Yanhao , Zhang He , Zhang Na , Chen Rui 
Published: April 2022

Abstract
Background
Berry size and early-ripening are considered as crucial traits for grape production and breeding. However, the underlying molecular mechanisms regulating berry size and early-ripening caused by bud sport in grapes remain unknown.
Results
In this study, whole-genome resequencing was performed for the cultivars ‘Himord Seedless’, ‘Thompson Seedless’ and their larger berries and early-ripening bud sports, ‘Jinzao Wuhe’ and ‘Dawuhebai’. Consequently, 1,548 SNPs, 379 InDels and 481 SVs, corresponding to 1,440 target genes, were identified in both bud sports compared to their maternal plants. Transcriptome analysis for ‘Jinzao Wuhe’/‘Himord Seedless’ at four time-points, including full-flowering, berry expansion, véraison and maturity stages, generated 2,984 differential expression genes (DEGs). Of these, 121 DEGs comprise genetic variations which might play potential roles in controlling berry growth and maturity development. Furthermore, weighted gene co-expression network analysis (WGCNA) was carried out, which revealed 48 candidate genes with previously discovered SNPs, InDels and SVs, including MADS, NAC, WRKY, UDP-glycosyltransferases, cytochromes P450, and genes involved in cell wall modification, ethylene synthesis and plant resistance. Finally, a conceivable regulatory network was constructed by integrating entire clues about the function of target genes.
Conclusions
Combined with the whole-genome resequencing and RNA-seq, DEGs with genetic variations associated with early ripening and berry size were identified in ‘Himord Seedless’ and its bud variant ‘Jinzao Wuhe’. Our results gave new insights into the field of bud mutation and contributed to future grape breeding and genetic improvement.
Augmented Renal Clearance in Patients with Traumatic Brain Injury Impacts Levetiracetam
Authors: Cook Aaron M. , Hall Kaylee , Kolpek Jimmi Hatton , Morbitzer Kathryn A. , Jordan J. Dedrick , Rhoney Denise H. 
Published: March 2022

Abstract
BACKGROUND: The purpose of this study was to compare serum concentrations of levetiracetam in patients with acute traumatic brain injury (TBI) during the first seven days following ICU admission. The hypothesis was levetiracetam concentrations are significantly lower in TBI patients with augmented renal clearance (ARC) compared to those with normal renal clearance.METHODS: This is a prospective cohort pharmacokinetic study of adults with moderate to severe TBI treated with levetiracetam during the first week after injury. Serial blood and urine collections were performed daily for analysis of levetiracetam, cystatin C, and creatinine clearance (CrCl) determinations. Patients were divided into two cohorts with (CrCl ³170ml/min/1.73m2) and without ARC.RESULTS: Twenty-two patients with moderate to severe TBI were included. The population consisted primarily of young male patients with severe TBI (mean age 40 years old, 68% male, median admission GCS 4). Each received levetiracetam 1000mg IV every 12 hours for the study period. ARC was present in 77.3% of patients, with the mean creatinine clearance in patients with ARC 170ml/min/1.73m2. Levetiracetam concentrations were significantly lower in patients with ARC than in those without ARC. Mean levetiracetam serum concentrations were below the typical therapeutic range for all study days in patients with ARC (< 6mcg/ml). In patients without ARC, the serum concentrations were also below the expected range on all but two study days which were days 4 and 5. Four of the 22 (18.2%) patients exhibited seizure activity during the study period (two of these patients exhibited ARC). Cystatin C concentrations were significantly lower in patients with ARC, though the mean for all patients was within the typical normal range.CONCLUSIONS: ARC has a high prevalence in patients with moderate to severe TBI. Levetiracetam concentrations after standard dosing were low in all TBI patients, but significantly lower in patients with ARC. This study highlights the need to consider personalized drug dosing in TBI patients irrespective of the presence of ARC.Clinical Trial Registration: This study was registered at cliicaltrials.gov (NCT02437838) Registered on May 8, 2015, https://clinicaltrials.gov/ct2/show/NCT02437838
The mothers' breastfeeding behavior within six weeks postpartum: new scale development and psychometric validation study
Authors: Wu Jing-Ling , Pang Shu-Qin , Jiang Xiu-Min , Lin Yan , Zheng Qing-Xiang 
Published: April 2022

Abstract
Background: The evaluation of mothers' breastfeeding behavior within six weeks postpartum could help health workers to identify maternal breastfeeding shortcomings comprehensively, clarify nursing problems, and provide targeted interventions. However, no prior study was found and the study aimed to develop and validate the reliability and validity of mothers' breastfeeding behavior scale within six weeks postpartum.Methods: The main two-step approach was used: (1) A qualitative pilot study using the purposive sampling method was adopted to test fitness, simplicity, and clarity of items with 30 mothers; (2) A cross-sectional survey using the convenient sampling method was conducted for item analysis and psychometric validation with 600 mothers.Results: The final version of the scale consisted of 36 items with seven dimensions, explaining 68.852% of the total variance. The Cronbach's α, split-half, and re-test coefficients were 0.958, 0.843, and 0.753, respectively. The validity of the scale: (1) Content validity: The content validity indexes (CVI) range of items were between 0.882 and 1.000. The scale-level-CVI was 0.990. (2) Structure validity: The fitting index were as follows: =2.239, RMR=0.049, RMSEA=0.069, TLI=0.893, CFI=0.903, IFI=0.904, PGFI=0.674, PNFI=0.763. (3) Convergent validity: The composite reliability and Average Variance Extraction (AVE) of 7 dimensions were between 0.876 and 0.920, 0.594 and 0.696. (4) Distinguish validity: The correlation coefficients were less than the square root of the AVE, excepting self-decision behavior, self-coping behavior, and self-control behavior. However, the fitting index of the original three-factor model was better than other new models with significant differences (P<0.001). (5) Calibration validity: The area under the curve was 0.860 or 0.898 when the scale was used to predict exclusive or any breastfeeding at 42 days. The correlation coefficient of maternal breasting feeding evaluation scale, breastfeeding self-efficacy short-form scale, and the scale were 0.569 and 0.674.Conclusion: The newly developed mothers' breastfeeding behavior scale within six weeks postpartum consists of thirty-six items belonging to seven dimensions with good reliabilities and validities, a reliable and valid instrument to be used in future maternal breastfeeding behavior assessments and interventions.
Breed distributions for diabetes mellitus and hypothyroidism in dogs
Authors: Ringstad Nanna Kristine , Lingås Frode , Thoresen Stein 
Published: March 2022

Abstract
Background: Diabetes mellitus (DM) and hypothyroidism are common canine endocrinopathies. Both primary (“Type 1”) DM and primary hypothyroidism are assumed to originate from autoimmune destruction of the respective endocrine glands and have been associated with the major histocompatibility complex (MHC) gene region. This study aims to investigate canine breed distributions for DM and hypothyroidism and calculate odds ratios (OR) for both diseases in the included breeds. Methods: Results from canine serum samples submitted from 2001-2018 to the Veterinary Clinical Pathology Laboratory (VCPL) at the Faculty of Veterinary Medicine, Norwegian University of Life Sciences for analysis of fructosamine and thyroid hormones in serum were used in a retrospective case-control study. OR was calculated as a measure of risk for the included breeds, where all the submitted blood samples to the VCPL and dogs registered in the Norwegian Kennel Club (NKK), the national organization for dog owners, were used as two alternative control populations. Results: Significant differences in disease incidence between breeds were discovered. Australian terrier, Swedish lapphund, Samoyed, and Schipperke were at highest risk for DM. German Shepherd, Golden retriever, German pointing dog, and Collie presented as the breeds with lowest risk for DM. For hypothyroidism, Schnauzer, Eurasier, Dunker, and English setter were at highest risk for developing the disease. The breeds at lowest risk of developing hypothyroidism were Rottweiler, Dachshund, German shepherd, and Border collie. Conclusions: These findings support that there is a strong genetic predisposition involved in the aetiology of these two diseases. Interestingly, there also appeared to be an inverse relationship of risk for the two diseases for some of the breeds since some breeds that had a high OR for DM or hypothyroidism have a lower OR for the other disease. This indicates that there may be different risk alleles/haplotypes for the two diseases. The possible etiological relationship between canine DM and hypothyroidism should be further investigated.
A Graph Model with Integrated Pattern and Query Based Technique for Extracting Answer to Questions in Community Question Answering System
Authors: Ojokoh Bolanle , Igbe Tobore , Afolabi Bamidele , Daramola Oladunni 
Published: April 2022

Abstract
In community question answering (CQA) systems, topical comments are very valuable to provide information for users. However, it becomes cumbersome going through all these in order to decipher the correct answers to particular questions. Hence, extracting a particular answer to a question becomes vital to avoid reading every comment in the forum. This paper is an extension of of our previous research work that extracted questions from an online forum to develop a system for answer extraction to questions. This system is based on a graph-based method by building answers for related questions using nKullback–Leibler (KL) divergence to obtain ranked answers to a question. The process of extracting answers to questions involves; question core, building question query, query-based answer extraction (QBAE), pattern-based answer extraction (PBAE), and combined answer extraction. The source data for this work were already existing data from ResearchGate, a socio-academic networking website that provides researchers the platform collaborate, ask question and offer answers to question. The performance for answer extraction for 2786 questions shows that when 80% of patterns and keywords were considered, QBAE and PBAE extracted 2765 and 2766 correct answers respectively, while the QBAE + PBAE method extracted 2782 correct answers. Also, when 90% of patterns and keywords were utilized, QBAE and PBAE extracted 2782 and 2784 correct answers, whereas the QBAE + PBAE method extracted 2786 correct answers. Our method was able to identify 229 questions without answers. Finally, the evaluation of our model reveals high-performance accuracy and precision.
Predictors of asthma control differ from predictors of asthma attacks in children: the Swiss Paediatric Airway Cohort
Authors: Ardura-Garcia Cristina , Mallet Maria Christina , Berger Daria Olena , Hoyler Karin , Jochmann Anja , Kuhn Alena , Moeller Alexander , Regamey Nicolas , Singer Florian , Pedersen Eva Sophie Lunde , Kuehni Claudia Elisabeth , Team on behalf of SPAC Study 
Published: March 2022

Abstract
Background
It is unclear if predictors of asthma attacks are the same as those of asthma symptom control in children. We evaluated predictors for these two outcomes in a clinical cohort study.
Methods
The Swiss Paediatric Airway Cohort (SPAC) is a multicentre prospective clinical cohort of children referred to paediatric pulmonologists. This analysis included 516 children diagnosed with asthma. At baseline, we collected sociodemographic information, symptoms, personal and family history and environmental exposures from a parental baseline questionnaire, and treatment and test results from hospital records. Outcomes were assessed 1 year later by parental questionnaire: asthma control in the last 4 weeks as defined by GINA guidelines, and asthma attacks defined as any unscheduled visit for asthma in the past year. We used logistic regression to identify and compare predictors for suboptimal asthma control and asthma attacks.
Results
At follow-up, 114/516 children (22%), reported suboptimal asthma control, and 114 (22%) an incident asthma attack. Only 37 (7%) reported both. Suboptimal asthma control was associated with poor symptom control at baseline, wheeze triggered by allergens, colds and exercise, a more intense treatment at baseline, history of preschool and persistent wheeze, and exposure to tobacco smoke. Incident asthma attacks were associated with previous episodes of severe wheeze and asthma attacks, younger age and non-Swiss origin. Lung function, exhaled nitric oxide (FeNO) and allergic sensitization at baseline were not associated with control or attacks.
Conclusion
Predictors of suboptimal control differ from those of attacks. Prediction tools and preventive efforts should differentiate these two asthma outcomes.
Three-dimensional nanofibrous sponges with controlled hierarchy regulating neural stem cell fate for spinal cord regeneration
Authors: Ni Shilei , Li Zhiwei , Qi Ye , Sun Lei , Li Zheng , Chen Shaojun , Zhang Yuqi , Ma Yuan , Han Jinming , Zhang Yulin , Geng Huimin , Huang Bin , Wang Jian , Li Gang , Li Xingang , Wu Shaohua 
Published: March 2022

Abstract
A strategy combining biomimetic nanomaterial scaffolds with neural stem cell (NSC) transplantation holds promise for spinal cord injury (SCI) treatment. In this study, innovative three-dimensional (3D) nanofibrous sponges (NSs) are designed and developed by a combination of directional electrospinning and subsequent gas-foaming treatment. The as-generated 3D NSs exhibit uniaxially aligned nano-architecture and a highly controllable hierarchical structure with high porosity, outstanding hydrophilicity, and reasonable mechanical performance, and they are demonstrated to facilitate cell infiltration, induce cell alignment, promote neuronal differentiation of NSCs, and enhance their maturation by activating the cellular adhesion molecule (CAM) pathways. The in vivo data show that NSC-seeded 3D NSs efficiently promote axon reinnervation and remyelination in a rat model of SCI, with new “neural relays” constructed across the lesion gap. The recovery of coordinated locomotion and sensory function was both significantly improved, accompanied by the restoration of ascending and descending electrophysiological signalling. Overall, the present study indicates that the as-fabricated 3D NSs can effectively regulate the fate of NSCs, and an advanced combination of 3D NS design and transplanted NSCs invites applications as an ideal tissue-engineered scaffold for SCI repair.
Inhibiting HMGB1 attenuates neuronal ferroptosis after spinal cord injury by inhibiting the p38/JNK MAPK signaling pathway
Authors: Wu Zhiwu , Wang Zhihua , Zhu Huaxin , Zhang Zhixiong , Li Meihua 
Published: April 2022

Abstract
Neuronal ferroptosis is an important pathological process after spinal cord injury (SCI). High mobility group box-1 protein (HMGB1) could regulate ferroptosis in other diseases. However, the effects of HMGB1 on neuronal ferroptosis after SCI are unclear. In this study, in vitro and in vivo experiments were performed to assess the expression levels of ferroptosis-related genes and proteins, as well as malondialdehyde (MDA) and iron levels. A recombinant HMGB1 plasmid was transfected into erastin-treated PC12 cells after Glycyrrhizic acid (GA) treatment to assess whether GA effects could be reversed. GA (100 mg/kg) was intraperitoneally injected into rats to establish an animal model; immunofluorescence, immunohistochemistry, Prussian blue and DAB staining assays were performed to assess GA effects in vivo. We found that GA treatment reversed the increases in levels of MDA, iron, HMGB1 expression, and Acyl-CoA synthetase long-chain family member 4 (ACSL4) expression and the decreases in glutathione peroxidase 4 (GPX4) levels. These protective effects were blocked by the presence of recombinant HMGB1. The inhibitory effects of GA on ferroptosis were partially influenced by inactivation of the p38/JNK signaling pathway. In conclusion, inhibiting HMGB1 attenuates neuronal ferroptosis after SCI may through p38/JNK MAPK signaling pathway inhibition in the early stages of SCI.
WITHDRAWN: Outcomes of Endoscopic Resection for Colorectal Polyps with High Grade Dysplasia or Intramucosal Cancer
Authors: Mohapatra Sonmoon , Almazan Erik , Charilaou Paris , Recinos Luisa , Bassi Mehak , Broder Arkady , Salimian Kevan , Khashab Mouen A , Ngamruengphong Saowanee 
Published: March 2022

Abstract

Purpose: Little is known about the outcomes of endoscopic resection (ER) for patients with colorectal adenomas (CRAs) with high-grade dysplasia (HGD) or intramucosal cancer (IMCA). This study aimed to estimate the rate of local/malignant recurrence, identify the predictive factors for local recurrence (LR) and evaluate the treatment outcomes of recurrence after ER for HGD/IMCA. Methods: A retrospective review was performed to identify patients who underwent ER for HGD/IMCA in two academic medical centers. Risk factors for LR were determined by Cox regression analysis.Results: Overall, 188 lesions with HGD/IMCA were included; 61 lesions were removed by en-bloc ER (e-ER), whereas 127 lesions were removed in a piecemeal ER (p-ER). The mean lesion size was 20.3 mm. Of the 125 patients who underwent follow-up, local adenoma recurrence occurred in 31 (23%), and malignant recurrence occurred in 2 (1.6%) patients at a median follow-up of 8 months. HGD/IMCA ≥ 4 cm removed by p-ER have the greatest hazard ratio (HR=21.5, 95% CI: 2.5-180.5; p=0.005) for LR, compared to the HGD/IMCA < 4 cm removed by e-ER. Surgery was performed in 3.2% of patients after a complete ER, all after p-ER. Of all patients who had LR, 22.6% (7/31 patients) had recurrent/residual adenomas despite repeat ER attempts after a mean of 1.9±0.79 procedures from the index ER. Conclusions: Our study demonstrates high rate of LR (23%) after ER of CRAs with HGD/IMCA with rate of malignant recurrence of 1.6% especially after p-ER. Thus, e-ER should be preferred for these lesions whenever technically feasible.
Seasonal distribution of cirrus cloud characteristics and their rapid descent from polarization lidar measurements at the west coast of India
Authors: Aswini M. A. , Ruchith R.D. , Das Subrata Kumar , Kumar Ashwini 
Published: April 2022

Abstract
Cirrus clouds are highly reflective clouds that play an important role in the atmospheric radiative balance and hence contribute to climate change. We present seasonal distribution of cirrus cloud occurrence, their characteristics and evolution over a coastal station (Goa, 15.4o N, 73.8o E, 40m above MSL) in India, using ground-based Micro Pulse lidar and Vaisala radiosonde observations from July 2013 to June 2015. The frequency of cirrus occurrence was observed around 44%, 33%, 17% and 6% in winter (JF), pre-monsoon (MAM), south west monsoon (JJAS) and post-monsoon (OND) respectively. Thicker cirrus were more frequent in September with an average value of > 1.7 ± 0.89 Km. The frequency of cirrus descent occurs almost similar in monsoon and post-monsoon (42% and 43% respectively) months. Two extreme cirrus descending cases were observed during the monsoon: (1) on 22 August 2014 with a displacement of 9.6 km from the initial height of 14 km with a descending rate and geometrical thickness of 0.4 ms− 1 and 1.5 km respectively; (2) on 14 September 2013 with a displacement of 7.8 km with descending rate and thickness of 0.2 ms− 1 and 2.3km respectively. Relatively high humidity (> 60%) and low (<-20 oC) ambient temperature caused thick cirrus formation in September 2013. However, during August 2014 event, relatively thinner (optically) cirrus was observed and the same is attributed to lower humidity conditions (45 to 60%). The wind shear associated with the tropical easterly jet (TEJ) appears to play a major role in cirrus displacement by modifying their vertical velocity. The positive and negative vertical velocity fields cause cirrus to a descending shape. The gradual descending pattern of cirrus is moving parallel to the vertical velocity contour, while the rapid descending in 2014 was mainly due to the occurrence of cirrus perpendicular to the velocity contour. Overall, this study represents the descending nature of cirrus clouds and their impact on the surface radiative characteristics over a coastal station in India.
DTI Analysis of White Matter Integrity and Cognitive Brain Reserve in Lifelong Musicians and Controls
Authors: Andrews Edna , Eierud Cyrus , Banks David , Harshbarger Todd , Michael Andrew , Rammell Charlotte 
Published: April 2022

Abstract
Previous research has focused on structural and functional changes to the human brain by training and experience in both linguistic (esp. bilingualism) and musical domains. While normal aging produces trends of decreasing WM integrity, research on cognitive brain reserve suggests that complex sensory-motor activities across the life span may slow down or reverse these trends. The current study incorporates findings from our recent DTI study of lifelong musicianship and building cognitive brain reserve and expands that analysis to include age and education matched non-musician controls. Reinforcing previous results, we show that non-musician controls in aging do not show the increases in WM integrity found in the subcortical tracts in lifelong musicians and that the difference between the groups was significant (p = 0.0065).
Novel compound heterozygous PLEC mutations lead to pure late-onset muscular dystrophy
Authors: Liu Mengyang , Lin Ying , Qiao Lingya , Chen Juan , Shi Qiang 
Published: April 2022

Abstract
Limb-girdle muscular dystrophy 2Q (LGMD2Q) is a specific mutation in the plectin (PLEC) gene at chromosome 8q24.3. The major phenotypes of plectinassociated disorder were epidermolysis bullosa with or without skeletal muscle involvement. The phenotype of pure muscular dystrophy was rarely reported. Here we report a Chinese patient carrying a novel compound heterozygous PLEC mutations presenting with pure muscular dystrophy.
Modified Centroid Triplet Loss for Person Re-Identification
Authors: Alnissany Alaa , Dayoub Yazan 
Published: March 2022

Abstract
Person Re-identification (Reid) is the process of matching target individuals to their images within different images or videos captured from a variety of angles or cameras. This is a critical task for surveillance applications, in particular, these applications that operate in large environments such as malls and airports. Recent studies use data-driven approaches to tackle this problem. This work continues on this path by presenting a modification of a previously defined loss, the centroid triplet loss (ctl). The proposed loss, modified centroid triplet loss (mctl), emphasizes more on the interclass distance. It is divided to two parts, one penalize for interclass distance and second penalize for intraclass distance. As a result of this adjustment, a new state-of-the-art results of 98.4% rank1, 98.63% mAP on market-1501, and 96.8% rank1, 97.3% mAP on DukeMTMC-Reid were achieved.