bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protected areas fail to cover the full annual cycle of migratory butterflies
Authors: Chowdhury Shawan , Cardillo Marcel , Chapman Jason , Green David , Norris Ryan , Riva Federico , Zalucki Myron , Fuller Richard 
Published: November 2022

Abstract
Effective conservation of migratory species relies on protection throughout their annual cycle1, something that has not been assessed globally for migratory insects. Here, we develop seasonal ecological niche models for 405 migratory butterfly species globally to assess whether they are adequately covered by protected areas (PAs) across their full annual cycle. PA coverage was inadequate in at least one season for 83% of migratory butterflies and 17% only achieved target representation in one season, and 43% did not meet the target representation in any season. While 95% of migratory butterflies met the target representation in Australia, only 1% did so in the Oriental region, indicating marked geographic variation in PA coverage. Coordinated efforts, across multiple countries, are needed to develop PAs that conserve migratory animals.
Dose-dependent proliferative and pro-oxidative effects of earthworm coelomic fluid on the HCT116 colon cancer cell line
Authors: Małota Karol , Urbisz Anna , Skonieczna Magdalena 
Published: November 2022

Abstract
Natural origin substances are an important source of medicinal compounds and have been used for centuries as remedies for many ailments. One source of these substances is earthworm (EW) coelomic fluid (CF). In this paper, we present the effects of earthworm Dendrobaena veneta CF on the HCT116, A549 and BEAS-2B cell lines. We used two fractions of CF, one heated to 70°C (CF-H) and unheated CF (CF-NH). Moreover, we used two different sets of doses: high doses (500, 250, 125, 62.5 µg/ml) and low doses (16, 8, and 4 µg/ml). Our results showed no cancer-selective cytotoxicity against any studied cell line in both CF-H and CF-NH groups. Interestingly, we discovered that low doses of CF-NH cause proliferative and pro-oxidative effects on the studied cell lines. These effects, to the best of our knowledge, are described here for the first time, and we believe it is an interesting new starting point in earthworm coelomic fluid studies.
Mixed Methods Evaluation of the Inaugural Year of the Cancer Prevention and Control Research Network’s (CPCRN) Scholars Program
Authors: Escoffery Cam , Petagna Courtney , Wangen Mary , Flicker Kimberly J. , Noblet Samuel B. , Sakhuja Mayank , Thomson Cynthia A. , Morrato Elaine H. , Adams Swann , Leeman Jennifer , Friedman Daniela B. 
Published: November 2022

Abstract
Purpose: A diverse workforce trained in dissemination & implementation (D&I) science is critical for improving cancer outcomes and reducing cancer-related health disparities. This study aims to describe and evaluate impact of the Cancer Prevention and Control Research Network (CPCRN) Scholars Program in preparing scholars for collaborative careers in cancer control and implementation research and practice, and offers evaluation-driven recommendations for program improvements.
Methods: The CPCRN Scholars Workgroup conducted a sequential, mixed methods evaluation. We collected baseline and follow-up surveys and invited all 20 scholars and 10 mentors to participate in an exit interview. We assessed the experience with the Scholar’s program, ratings of D&I competences, progress on their project, feedback about the curriculum, and understanding of implementation science.
Results: Over 86% partially or fully completed their project within 9 months; 78% of scholars engaged with a CPCRN workgroup. Scholars rated the following program components as valuable: the Putting Public Health Evidence in Action (PPHEIA) training (88.9%), D&I training modules (83.3%), and webinars (kickoff webinar-88.9% and selecting theories/models-88.9%). There was an increase in D&I competencies from baseline to posttest, with the greatest in community engagement topics. About 78% reported that they were satisfied with format of the activities and increased confidence in ability to discuss D&I concepts. From the qualitative interviews, the benefit of the program was becoming more knowledgeable about D&I research and networking.
Conclusion: The inaugural year of the program yielded positive results, particularly related to increasing knowledge about D&I science and cancer control. This program builds the capacity of students, researchers and practitioners in D&I science.
Evaluation of Glutathione S-transferase Mu 3 (GSTM3) levels in sperm as a simple method to predict oxidative DNA damage and seminogram alterations
Authors: Llavanera Marc , Ribas-Maynou Jordi , Mateo-Otero Yentel , Benet Jordi , Bonet Sergi , Yeste Marc 
Published: November 2022

Abstract
Background. Previous evidence suggested sperm Glutathione S-transferase Mu 3 (GSTM3) to be essential for an appropriate mitochondrial function, plasma membrane stability and oxidative regulation of mammalian sperm. In humans, however, neither has this enzyme been related to semen alterations nor has it been reported to be associated to oxidative DNA damage and (in)fertility.
Methods. The aim of the present study was to assess the utility of GSTM3 to predict spermiogram alterations and oxidative DNA damage in sperm. A total of 34 semen samples were collected, evaluated by conventional semen analysis, and subsequently cryopreserved. Frozen-thawed sperm samples were assessed for DNA fragmentation and the presence, localization and content of GSTM3.
Results. Sperm GSTM3 content was positively associated to sperm quality parameters (P < 0.05). Specifically, lower levels of this antioxidant enzyme were observed in asthenoteratozoospermic and oligoasthenoteratozoospermic men (P < 0.05), but not idiopathic infertile patients (P > 0.05), when compared to normospermic samples. Moreover, sperm GSTM3 was negatively associated to oxidative DNA damage (P < 0.05). Finally, the ability to predict spermiogram alterations was determined by ROC curve analysis using GSTM3 alone (AUC of 0.89; P < 0.05), and in combination with oxidative DNA damage (AUC of 0.91; P < 0.05).
Conclusions. Although the limited sample size of the present study warrants further studies and clinical trials with larger sample sets, our results set the ground for using GSTM3 as a novel biomarker and therapy target for male infertility and oxidative DNA damage in sperm.
A comprehensive expression profiles and prognostic values analysis of inosine 5'-monophosphate dehydrogenase type II (IMPDH2) in human tumors Running title: A pan-cancer analysis of IMPDH2
Authors: Huang Zigao , Cao Jiahua , Lao Jingmao , Deng Wei 
Published: November 2022

Abstract
Background
Inosine 5'-monophosphate dehydrogenase type II (IMPDH2) was thought to be involved in cancer initiation, progression, and treatment, but its biological role and underlying mechanism in pan-cancers are not fully studied. Our goal was to systematically investigate expression status and prognostic value through online data mining.
Methods
The expression and prognostic value of IMPDH2 in pan-cancer were analyzed using the HPA, GEPIA, TIMER, UALCAN, PrognoScan, Kaplan–Meier Plotter and DriverDBv3, and confirmed by immunohistochemical staining in gastric cancer. Function analysis was determined with GeneMANIA, STRING, and Metascape interactive tools, and alterations were examined by cBioPortal and UCSC Xena. Moreover, TISIDB and TIMER were applied to explore the relationships between IMPDH2 expression and immune infiltration, subtypes.
Results
Upregulation of IMPDH2 was observed in multiple human cancer tissues and relevant to a poor survival. Interactive analysis indicated that IMPDH2 was significantly associated with the metabolism of nucleotides, nucleobase metabolic process and regulation of canonical Wnt signaling pathway. Notably, a heatmap revealed positive correlations of IMPDH2 expression with macrophage M2, common lymphoid progenitor, myeloid derived suppressor cells, and negative correlations with macrophage M1, T cell follicular helper, B cell, T cell CD8+, dendritic cell. Additionally, there was a significant association between the IMPDH2 copy number, DNA methylation, exon expression levels and disease-free interval.
Conclusions
Together, IMPDH2 could act as a novel biomarker for the prognostic and immunological role in multiple human cancers.
The pre-surgical role of halo-traction in patients with cervical infection associated with refractory kyphosis. A retrospective study
Authors: Manini Daudi R. , Zhang Hong-Qi , Gao Qile , Liu Shao-Hua , YuXiang Wang , Tang Ming-Xing , An Deng , Guo Chao-Feng , YuXuan Du 
Published: November 2022

Abstract
To minimize surgical complications and staged procedures halo-traction is often used during deformity corrections. But the use of halo-traction in the treatment of rigid cervical kyphosis secondary to infections with abscesses has never been reported. This study investigated the role of halo-traction in the treatment of cervical infection patients associated with refractory kyphosis. We retrospectively reviewed 48 patients with cervical infection associated with refractory kyphosis who were treated in our spine department. Patients were divided into two groups, the traction group (A) and the non-traction group (B). Group A underwent preoperative halo-traction followed by surgery, while group B underwent surgery alone. Between the two groups, we analyzed the kyphosis deformity correction, level of fusions, disease clearing rate by erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), pain improvement by Oswestry disability index (ODI), and complications. Group A had a better correction of kyphosis deformity compared to group B (10.26±3.61)0 versus（12.24± 2.4)0 (P=0.04) ). No statistically significant differences between the two groups in terms of disease-clearing rate, pain improvement, level of fusion, and complications. Preoperative halo-traction followed by surgery is superior in kyphosis correction in the treatment of patients with cervical infections with refractory kyphosis.
A Novel Hybrid Arithmetic Optimization Algorithm and Salp Swarm Algorithm for Data Placement in Cloud Computing
Authors: awad ahmed , D.Abdellatif Ashraf , Alburaikan Alhanouf , Khalifa Hamiden , Elaziz Mohamed Abd , Abualigah Laith , M.AbdelMouty Ahmed 
Published: November 2022

Abstract
In recent years, the Internet of Things (IoT) has led to the spread of cloud computing devices in all commercial, industrial and agricultural sectors. The use of cloud computing environment services is increasing exponentially with all technology applications based on IoT. Fog computing has led to addressing issues in cloud computing environments. Fog computing reduces load balancing, processing, bandwidth, and storage as data file replication from the cloud to the network closest to sensors in different geographic locations. Traditional cloud computing leads to an increase in response time and processing time, and processing in the performance of data replication. We need replication strategies to meet users' requirements across different geographic locations while effectively harnessing fog computing capabilities to optimally select and place data replication of IoT services on cloud resources. In this strategy, the identification and mode of the data replication problem are designed as a multi-objective optimization problem that considers the heterogeneity of resources, least cost path, distance, and applications based on replication requirements. Firstly, a new hybrid metaheuristic method, using the Arithmetic Optimization Algorithm (AOA) and the salp swarm algorithm (SSA), is proposed to handle the problem of selection and placement data replication in fog computing. This strategy, called AOASSA, depends on using fog computing to optimally select and place data replication of IoT services on cloud resources. Secondly, the Floyd algorithm is used to strategy the least cost path, distance, and data transmission in different geographic locations. To validate the AOASSA strategy a set of experiments was carried out to validate the proposed strategy AOASSA. The performance of AOASSA is tested and compared with other swarm intelligence. Experiment results show the superiority of AOASSA over its competitors in terms of performance measures, such as least cost path, distance, and bandwidth.
Analysis and Pharmaceutical Applications of Serine protease from Arachis hypogaea
Authors: Shinde Shivam , Bhatia Manish S. , Halingale Omkar S. 
Published: November 2022

Abstract

Introduction:Serine protease is demonstrated to exist in both eukaryotes and prokaryotes. Serine proteases are present in the genomes of all cellular life forms, including many viruses, and are widely distributed in nature. Proteolytic enzymes that have attracted the most research attention are serine proteases.
Aim: This article highlights we have analysed the enzyme with various pH and temperature also developed the applications of serine protease enzyme.
Result:Serine protease enzyme is most stable at neutral ph i.e. pH &7.5 to 8 and at -4 ⁰C to 0 ⁰C. Critical analysis of the knowledge about serine protease from Arachis hypogaea source can help in the creation of pharmaceutical applications.
Discussion:The primary goals of the current effort are the creation of assays and the isolation of serine protease for pharmaceutical use. The enzyme serine protease is used to diagnose hemorrhagic or thrombotic disease co-agulation factors levels may also require. Targeted source was chosen, and the extraction procedure was constructed to control variables like pH and temperature for the right condition of the enzyme.
Conclusion:
Serine proteases were successfully isolated from Groundnut. Qualitative and quantitative characterization of the isolated serine proteases yielded consistent results.
The Representation of Greenery in the Boundaries Between the Open Landscape and Residential Areas in Suburbanized Rural Settlements. Development From the 19th to the 21st Century
Authors: Maňas Jan , Kabrhel Jan , Kyselovič Jakub 
Published: November 2022

Abstract
The rapid development of rural settlements into commuter towns in the hinterland of large cities, coupled with intensive agriculture and climate change, may negatively affect the prosperity of these areas. The presented analysis focused on randomly selected rapidly growing settlements in the Prague metropolitan area, examining their development with a focus on the peripheral parts of such settlements. Settlement margins constitute a specific part of rural settlements as it typically contributes to the rural character by providing the gradual gradient between the settlement and the open landscape.
The representation of water-absorbing and non-water absorbing areas and their suitability for the development of taller vegetation along the margins of three randomly selected suburbanized settlements in the hinterland of Prague are analysed from the perspective of the possibility to maintain the traditional character of the settlement margins. Historical maps, aerial photographs, spatial planning documentation and documents, and data on current land use and landscape cover were used for the analysis of the settlements’ development over the last approx. 200 years.
We have observed the trend of decreasing plot size and of reduction of the water-absorbing surfaces suitable for planting taller vegetation, especially in the 21st century residential developments, which disrupts the traditional rural character of these settlements. The utilization and stabilization of the current vegetation in the historical areas, together with the creation of the vegetation belts along the margins of the newly developed areas, could improve the environmental quality of these settlements, promote ecosystem services and increase biodiversity.
Application of radical mastectomy in ambulatory surgery centres under COVID-19
Authors: Zhou Jiao , Xiao Xiaoxiao , Yang Jiabin , Feng Yu , Qin Xiangquan , Li Juan , Yang Huanzuo , Qiu MengXue , Liu Yang , Huang Mingjun , Liang Peng , Du Zhenggui 
Published: November 2022

AbstractBackground Breast cancer has surpassed lung cancer as the most common cancer. Day surgery for breast cancer has been widely carried out worldwide, but the development of day surgery in China is relatively slow. To reduce the spread of coronavirus 2019 (COVID-19), major surgery centres have optimized the management procedures of day surgery to different degrees. However, relevant research on whether the rapid turnover of day surgery and the excessive human resources placed in the prevention and control of the COVID-19 epidemic will affect the quality of day surgery is lacking. Method The demographic data, clinical data and postoperative complications of breast cancer patients in the single medical group of West China Hospital of Sichuan University from March 2020 to June 2021 were retrospectively collected, and the complications after discharge and the safety of day surgery were analysed. Results The average age significantly differed between the ward surgery group (WS) and the day surgery group (DS) (P = 0.030). Regarding postoperative complications, no significant differences were detected in total surgical complications (P = 0.676) or anaesthesia complications (P = 0.126) between the two groups. In the logistic analysis, day surgery was not a risk factor for postoperative complications during the COVID-19 pandemic (P = 0.676, OR = 1.154, 95% CI: 0.590–2.257). An increase in age significantly increased the incidence of postoperative surgical complications (P = 0.024, OR = 1.051, 95% CI: 1.007–1.097). At the same time, lymph node dissection after sentinel lymph node biopsy also led to an increase in the incidence of postoperative surgical complications (P = 0.030, OR = 3.372, 95% CI: 1.125–10.106). In the survival curve, no significant difference in DFS was detected between the two groups (P = 0.353). Conclusion Radical mastectomy at day surgery centres is safe and reliable under strict COVID-19 management guidelines.
Multiple Adverse Events of anti-PD1/PDL1 Therapy: A Case- control Study using a Japanese Prescription-diagnostics Database
Authors: Hamano Hirofumi , Miyata Koji , Hara Tomoyo , Sakaguchi Satoshi , Oura Masahiro , Niimura Takahiro , Aizawa Fuka , Yagi Kenta , Okada Naoto , Miki Hirokazu , Goda Mitsuhiro , Ishizawa-Izawa Yuki , Zamami Yoshito , Yanagawa Hiroaki , Ishizawa Keisuke 
Published: November 2022

Abstract
Purpose
Accumulation of data on patient response to immune checkpoint inhibitors (ICIs) has enabled the analysis of adverse events (AEs) that have been difficult to analyze to date. The development of multiple AEs is often fatal; therefore, their characterization is important. Hence, we retrospectively investigated anti-programmed cell death protein 1 (PD1)/ programmed death-ligand 1 (PDL1) therapy by conducting a case-control study to determine the clinical characteristics of AEs associated with multiple ICIs.
Methods
We utilized the Japan Medical Data Center claims database, a prescription-diagnostics database, to obtain records of patients treated with PD1/PDL1 inhibitors, which were then analyzed. A total of 116,091 ICI-associated prescription-diagnostics data reports were retrieved. ICI-associated safety reports of individual cases till November 2020 were also analyzed. Furthermore, a narrowed search for immune-related AEs (irAEs) yielded 3,173 cases from the prescription-diagnostics data.
Results
There was a high incidence of concomitant AEs, and endocrine dysfunctions (hypophysitis, thyroiditis, and adrenal complications) had a high co-occurrence rate. Diabetes mellitus, hepatitis, and colitis overlapped in patients who developed thyroid dysfunction. In addition, there was a high incidence of myositis, myasthenia gravis, and myocarditis co-occurrence. Further analysis revealed a high mortality rate for interstitial pneumonia and diabetes mellitus (38%). In concomitant cases of myocarditis and other irAEs, severe cardiac diseases were developed.
Conclusion
Anti-PD1/PDL1 therapy may cause fatal clinical manifestations when accompanied by multiple irAEs. This study may provide adequate awareness among healthcare providers toward these characteristics.
Comprehensive study on clinical features, prognoses and histogenesis of combined hepatocellular-cholangiocarcinoma
Authors: Hu Junwen , Chen Jiahao , Yang Chenglei , Qi Yapeng , Zhang Jie , Zhong Jianhong , Xiang Bangde 
Published: November 2022

Abstract
Background:Combined hepatocellular-cholangiocarcinoma (CHC) is a rare subtype of primary liver cancer with histologic features of both hepatocellular carcinoma (HCC) andintrahepatic cholangiocarcinoma (ICC). The clinicopathological features，prognoses and histogenesis of CHC is unknown because of its low morbidity.
Methods: Twenty six patients with CHC, 901 with HCC, and 40 with ICC were included into analysis in this retrospective study. Their clinicopathological characteristics and prognosis were compared.
Results: CHC had clinicopathological features of both HCC and ICC. Patients with CHC had similar overall and disease-free survival with those with ICC, but all had significantly lower than those with HCC. Univariate analysis showed that AFP, CA19-9, CEA, tumor size, macrovascular invasion and lymph node metastasis were risk factors of both overall and disease-free survival. Multivariate analysis identified macrovascular invasion as independent prognostic factors for patients with CHC. Immunohistochemistry showed that CHC patients were with highly expression of CD90 and EpCAM.
Conclusions: Patients with CHC appear to have an intermediate clinical features compared with the HCC and ICC patients. The high expression of hepatic progenitor cell (HPC) markers in tumor tissues suggests that CHC may originate from HPCs.
AHP and GIS based land suitability analysis for built up in Bharatpur Metropolitan City, Nepal
Authors: Lamichhane Sadhuram 
Published: November 2022

Abstract
In Bharatpur Metropolitan City (BMC), rapid changes in socioeconomic conditions led to rapid urban growth, which in turn caused a number of environmental damages, including land fragmentation, unauthorized settlements, unchecked land use conversion, rising environmental pollution, and anthropogenic disasters. The rate of migration has been dramatically rising recently. These forecasts illustrated the gradual migration of people from rural to urban locations. Arable land fragmentation and erratic land use shifts are greatly impacted by rapid urbanization. They are significant concerns for property developers and urban planners. Urban planners and developers are unable to foresee city growth and modeling in the future using accurate management techniques due to unpredictable urban expansion. GIS based multi criteria decision making (MCDM) for land suitability analysis. Analytic hierarchy process (AHP) is a combination of qualitative and quantitative method used to calculate the weights of multi criterion by using pairwise matrices. By this method the biophysical and socioeconomic factors underlying the expansion has identified. Suitability maps had been prepared for several classes: highly suitable, moderate suitable, less suitable and restricted area and its percentage of area.