bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green Carbon Science: Keeping the Pace in Practice
Authors: Gao Peng , Zhong Liangshu , Han Buxing , He Mingyuan , Sun Yuhan 
Published: August 2022

AbstractThe concept of green carbon science is to promote the goal of achieving carbon neutrality, the importance and urgency of which have been well‐recognized and received worldwide attention. Carbon neutrality has become the focus of research work in many fields, and correspondingly, a growing number of papers and publications have been published over the last few years. However, since carbon neutralization is a real problem that urgently requires technical solutions, the transition from fundamental research to practical applications must be accelerated. A number of research teams are bold in putting things into practice and blazing new trails. This Review summarizes the recent technological developments within the framework of green carbon science, focusing on conducted pilot tests, industrial demonstrations, and commercial applications beyond the laboratory stage.
Identification and transcriptional profile of Culex quinquefasciatus Say (Diptera: Culicidae) lipophorin in different stages
Authors: Martins Larissa Almeida , de Moura Alexandre Santos , de Miranda Ceres Maciel , Cardoso André Franco 
Published: August 2022

AbstractLipophorin is a major hemolymph lipoprotein found in insects with a molecular native mass of 700 kDa. In mosquitoes, two different types of apolipoproteins are characterized, apolipophorin‐I (ApoLp‐I, ~250 kDa) and apolipophorin‐II (ApoLp‐II, ~80 kDa). This concentration depends on the stage of development and the age of the insects. Lipophorins are best studied in mosquitoes of the genus Aedes and Anopheles. In this study, we analyze the lipophorin sequence and show the lipophorin purification of the Culex quinquefasciatus and the transcriptional profile of the lipophorin gene in different life cycle stages. Similar amino acid composition and molecular weights are founded in three mosquitoes species lipophorins amino acid sequence. The two subunits of purified lipophorin (Apo I and Apo II) showed molecular masses of approximately 248 and 93 kDa, like that found in other mosquitoes. A gradual increase in the lipophorin expression gene was obtained during the previtellogenic period and after feeding we obtained peak expression at 24 h after feeding. With our results, we conclude that C. quinquefasciatus protein sequence has the same characteristics as those observed in other mosquitoes and that the expression of its apolipophorins is induced by blood feeding.
Development and External Validation of a Model Predicting New‐Onset Chronic Uveitis at Different Disease Durations in Juvenile Idiopathic Arthritis
Authors: van Straalen Joeri W. , Kearsley‐Fleet Lianne , Klotsche Jens , de Roock Sytze , Minden Kirsten , Heiligenhaus Arnd , Hyrich Kimme L. , de Boer Joke H. , Lamot Lovro , Olivieri Alma N. , Gallizzi Romina , Smolewska Elzbieta , Faugier Enrique , Pastore Serena , Hashkes Philip J. , Herrera Cristina N. , Emminger Wolfgang , Consolini Rita , Wulffraat Nico M. , Ruperto Nicolino , Swart Joost F. ,   the Paediatric Rheumatology International Trials Organisation (PRINTO), UK CAPS study, and German ICON study
Published: August 2022

ObjectiveTo develop and externally validate a prediction model for new‐onset chronic uveitis in children with juvenile idiopathic arthritis (JIA) for clinical application.MethodsData from the international Pharmachild registry were used to develop a multivariable Cox proportional hazards model. Predictors were selected by backward selection, and missing values were handled by multiple imputation. The model was subsequently validated and recalibrated in 2 inception cohorts: the UK Childhood Arthritis Prospective Study (CAPS) study and the German Inception Cohort of Newly diagnosed patients with juvenile idiopathic arthritis (ICON) study. Model performance was evaluated by calibration plots and C statistics for the 2‐, 4‐, and 7‐year risk of uveitis. A diagram and digital risk calculator were created for use in clinical practice.ResultsA total of 5,393 patients were included for model development, and predictor variables were age at JIA onset (hazard ratio [HR] 0.83 [95% confidence interval (95% CI) 0.77–0.89]), ANA positivity (HR 1.59 [95% CI 1.06–2.38]), and International League of Associations for Rheumatology category of JIA (HR for oligoarthritis, psoriatic arthritis, and undifferentiated arthritis versus rheumatoid factor–negative polyarthritis 1.40 [95% CI 0.91–2.16]). Performance of the recalibrated prediction model in the validation cohorts was acceptable; calibration plots indicated good calibration and C statistics for the 7‐year risk of uveitis (0.75 [95% CI 0.72–0.79] for the ICON cohort and 0.70 [95% CI 0.64–0.76] for the CAPS cohort).ConclusionWe present for the first time a validated prognostic tool for easily predicting chronic uveitis risk for individual JIA patients using common clinical parameters. This model could be used by clinicians to inform patients/parents and provide guidance in choice of uveitis screening frequency and arthritis drug therapy.
One‐Shot Active Learning for Globally Optimal Battery Electrolyte Conductivity**
Authors: Rahmanian Fuzhan , Vogler Monika , Wölke Christian , Yan Peng , Winter Martin , Cekic‐Laskovic Isidora , Stein Helge S. 
Published: August 2022

AbstractNon‐aqueous aprotic battery electrolytes need to perform well over a wide range of temperatures in practical applications. Herein we present a one‐shot active learning study to find all conductivity optima, confidence bounds, and relating formulation trends in the temperature range from −30 °C to 60 °C. This optimization is enabled by a high‐throughput formulation and characterization setup guided by one‐shot active learning utilizing robust and heavily regularized polynomial regression. Whilst there is an initially good agreement for intermediate and low temperatures, there is a need for the active learning step to improve the model for high temperatures. Optimized electrolyte formulations likely correspond to the highest physically possible conductivities within this formulation system when compared to literature data. A thorough error propagation analysis yields a fidelity assessment of conductivity measurements and electrolyte formulation.
The XK plasma membrane scramblase and the VPS13A cytosolic lipid transporter for ATP‐induced cell death
Authors: Ryoden Yuta , Nagata Shigekazu 
Published: August 2022

AbstractExtracellular ATP released from necrotic cells in inflamed tissues activates the P2X7 receptor, stimulates the exposure of phosphatidylserine, and causes cell lysis. Recent findings indicated that XK, a paralogue of XKR8 lipid scramblase, forms a complex with VPS13A at the plasma membrane of T cells. Upon engagement by ATP, an unidentified signal(s) from the P2X7 receptor activates the XK‐VPS13A complex to scramble phospholipids, followed by necrotic cell death. P2X7 is expressed highly in CD25+CD4+ T cells but weakly in CD8+ T cells, suggesting a role of this system in the activation of the immune system to prevent infection. On the other hand, a loss‐of‐function mutation in XK or VPS13A causes neuroacanthocytosis, indicating the crucial involvement of XK‐VPS13A‐mediated phospholipid scrambling at plasma membranes in the maintenance of homeostasis in the nervous and red blood cell systems.
Synthesis of mesoporous SiO2 coating containing chlorine phenol–formaldehyde resin (Cl–PFR) composites for effective fingerprint detection
Authors: Yang Ping , Sun Wei , Zhang Zikuan , Xing Honglong 
Published: August 2022

AbstractAs a type of nonmetals fluorescent reagent, the described chlorine phenol–formaldehyde resin (Cl–PFR) nanoparticles (NPs) were synthesized using a facile method. The as‐synthesized Cl–PFR NPs can emit strong green fluorescence emission under 365 nm ultraviolet (UV) light irradiation. As mesoporous silica (MSN) NPs have a large specific area, strong adsorption, and uniform dispersion, MSN‐coated Cl–PFR composites were prepared by mixing Cl–PFR and MSN NPs together. The as‐synthesized multifunctional composites combining the advantages of the green fluorescence of Cl–PFR, and the strong adhesion of MSN was applied to detect potential fingerprints. Different base fingerprints (glass, paper, aluminium sheets, rough stones, tape) could be clearly observed in the presence of the Cl–PFR@MSN–NH2 composites. Furthermore, fingerprints that had been aged for 3 months and washed with water several times could also be clearly displayed using the multifunctional composites. This study provided a simple, economical, and nontoxic fluorescent reagent for application in fingerprint detection.
An LC–ESI–MS/MS assay for the therapeutic drug monitoring of 15 antiseizure medications in plasma of children with epilepsy
Authors: Zhang Yuan‐Yuan , Xia Ying , Guo Hong‐Li , Hu Ya‐Hui , Wen Xiao‐Yi , Chen Jing , Lu Xiao‐Peng , Wang Shan‐Shan , Qiu Jin‐Chun , Chen Feng 
Published: August 2022

AbstractOral antiseizure medications are the preferred option for the clinical treatment of epilepsy. Therapeutic drug monitoring has become an important means of achieving individualized treatment of epilepsy. A sensitive, accurate and rapid LC–ESI–MS/MS method was developed and validated for the simultaneous determination of 15 antiseizure medications in human plasma (carbamazepine, gabapentin, pregabalin, phenytoin, zonisamide, oxcarbazepine, tiagabine, lamotrigine, topiramate, phenobarbital, lacosamide, primidone, 10,11‐Dihydro‐10‐hydroxy carbamazepine, ethosuximide, and levetiracetam). The sample preparation procedure was an one‐step protein precipitation with methanol. Mass detection was performed in ionization polarity switching mode (positive–negative–positive) using multiple reaction monitoring mode. A “boot‐shaped” gradient elution program was applied to separate and concentrate those target analytes, resulting in symmetrical peak shapes within 10 min, without endogenous interference. The method showed great linearity over the concentration ranges with acceptable correlation coefficients (0.9966–0.9996). The precision and accuracy values for intra‐ and inter‐assays were within ±15%. Consequently, the method was successfully implemented on pediatric patients undergoing mono‐ or polytherapy for epilepsy and provided timely concentration results to ordering clinicians.
Multidisciplinary approaches to gliosarcoma: A case report and review of the literature
Authors: Labak Collin M. , Rabah Nicholas M. , Kipke Jasmine P. , Mahajan Uma V. , Labak Kyle B. , Ali S. Ahmed , Fowler Nicole , Sloan Andrew E. 
Published: August 2022

AbstractA 58‐year‐old right‐handed man presented to our tertiary care center with gliosarcoma (GS) infiltration through the dura, skull, and soft tissue. Patient had a previous history of right temporal GS, with four intracranial surgeries prior to presentation. A multidisciplinary approach was used to treat the lesion and perform reconstruction.
The Effects of Material Extrusion Printing Speed on the Electrochemical Activity of Carbon Black/Polylactic Acid Electrodes**
Authors: Shergill Ricoveer Singh , Patel Bhavik Anil 
Published: August 2022

AbstractMaterial extrusion printing process can make electrodes and electronic components at any geometry and has provided a reproducible approach towards the fabrication of conductive carbon thermoplastic composite parts. Printing parameters can have a significant influence on the conductivity of the printed part, however limited studies have focused on understanding the impact of printing parameters. Our study explored the influence of printing speed on the electrochemical activity of 3D printed carbon black/polylactic acid (CB/PLA) electrodes. We made CB/PLA at print speeds ranging from 20 to 100 mm/s and evaluated the performance of these electrodes using cyclic voltammetry and through imaging. Electrodes made using 60 mm/s printing speed had the greatest current and electron transfer kinetics. Electrodes made using higher and lower printing speeds were more resistive. This study is the first to demonstrate the significant impact that printing speed can have on the electrochemical activity of 3D printed CB/PLA electrodes. The implications of this study are important when defining the 3D printing manufacturing process of electrodes and electronic components.
Direct Synthesis of 3‐Aryl Substituted Isocoumarins and Phthalides through Palladium Acetate Catalyzed C(sp2)−H Activation in Ionic Liquids
Authors: Aloia Andrea , Casiello Michele , D'Accolti Lucia , Fusco Caterina , Nacci Angelo , Monopoli Antonio 
Published: August 2022

AbstractA novel Pd‐catalysed oxidative coupling between benzoic acids and vinylarenes or acrylates to furnish isocoumarins and phthalides is reported. The reaction proceeds smoothly in molten tetrabutylammonium acetate via a selective C−H bond activation, with very low percentage of ligand‐free palladium acetate as the catalyst, under atmospheric pressure of oxygen. Sub‐stoichiometric amount of copper acetate is also required as a reoxidant for the palladium.
Assessment of the compliance, influencing factors, and yielding results of liver cancer screening in a high‐risk population: A cross‐sectional study
Authors: Cao Maomao , Li He , Sun Dianqin , He Siyi , Yang Fan , Yan Xinxin , Zhang Shaoli , Zha Zhenqiu , Zhou Jinyi , Luo Pengfei , Gong Jiyong , Ma Hengmin , Zhang Shaokai , Sun Xibin , Xia Changfa , Peng Ji , Chen Wanqing 
Published: August 2022

BackgroundThe participation and results for liver cancer screening are rarely reported. The aim of this study was to determine the participation rates and factors affecting participation rates as well as to report the detection rate for liver cancer in an organized screening program.MethodsThe organized screening program for liver cancer was conducted in 12 rural sites. The risk of developing liver cancer was initially evaluated for each participant. High‐risk individuals were offered α‐fetoprotein measurement and ultrasonography examination. Potential risk factors associated with the participation rate were screened by fitted generalized linear mixed logistic regression models through reporting odds ratios (ORs) with 95% CIs.ResultsA total of 358,662 eligible participants completed the basic surveys, and 54,745 were evaluated to be at high risk of liver cancer. Of these high‐risk individuals, 40,543 accepted the screening services. Determinants of participation for screening behavior included older age, being female, being positive for hepatitis B surface antigen, having a family history of liver cancer, chronic depression, and low income. The detection rate for liver cancer was estimated to be 0.41% (95% CI, 0.35–0.48).ConclusionsThis study reported several significant factors associated with the screening behaviors for liver cancer.Lay Summary

Participation rate and results for liver cancer screening in rural areas are rarely reported.

The determinants associated with adherence rates and early detection rate of liver cancer in an organized screening program for liver cancer were assessed.

A possible positive correlation between the participation rates and the early detection rate was observed among attendees of screening. These new finds could be beneficial to increasing the participation rate of screening.
Perceptions of patient disease burden and management approaches in systemic mastocytosis: Results of the TouchStone Healthcare Provider Survey
Authors: Mesa Ruben A. , Sullivan Erin M. , Dubinski David , Carroll Brittany , Slee Valerie M. , Jennings Susan V. , Finnerty Celeste C. , Bohannon Linda S. , Mathias Susan D. , Lahue Betsy J. , Castells Mariana C. 
Published: August 2022

BackgroundSystemic mastocytosis (SM) is a rare clonal neoplasm driven by the KIT D816V mutation and has a broad range of debilitating symptoms. In this study, the authors evaluated SM disease perceptions and management strategies among US health care providers (HCPs).MethodsHematologist/oncologist (H/O) HCPs and allergist/immunologist (A/I) HCPs who were treating four or more patients with SM completed an online, 51‐item TouchStone HCP Survey, which queried provider characteristics, perceptions of disease burden, and current management. Descriptive analyses by specialty and SM subtype were performed.ResultsOf 304 HCPs contacted, 111 (37%) met eligibility criteria, including 51% A/I specialists and 49% H/O specialists. On average, the HCPs had 14 years of practice experience and cared for 20 patients with SM. A/I HCPs saw more patients with nonadvanced SM (78%) compared with H/O HCPs, who saw similar proportions of patients with nonadvanced SM (54%) and advanced SM (46%). HCPs reported testing 75% of patients for the KIT D816V mutation and found an estimated prevalence of 47%. On average, HCPs estimated 8 months between symptom onset and SM diagnosis. HCPs reported that 62% of patients with indolent SM felt depressed or discouraged because of symptoms. In terms of treatment goals for SM, both types of specialists prioritized symptom improvement for nonadvanced SM and improved survival for advanced SM while also prioritizing improving patient quality of life.ConclusionsBoth A/I and H/O specialists highlighted unmet needs for patients with SM. The HCPs surveyed reported a lower rate of KIT D816V mutations and a perceived shorter time between symptom onset and SM diagnosis compared with published estimates.Lay summary

Specialists treating systemic mastocytosis (SM) completed a 51‐item questionnaire about their clinical practices and perceptions of disease impact.

The study included 111 hematology, oncology, allergy, and immunology physicians.

Physicians reported that most patients had nonadvanced disease, yet SM symptoms significantly disrupted their patients' lives.

Physicians estimated that SM is diagnosed within months of symptom onset, in contrast with published reports of years' long delays reported by patients with SM.

This study identified unmet needs that can inform educational and patient management priorities in this rare disease.
Biomechanical analysis of lumbar nonfusion dynamic stabilization using a pedicle screw‐based dynamic stabilizer or an interspinous process spacer
Authors: Fan Wei , Zhang Chi , Zhang Dong‐Xiang , Guo Li‐Xin , Zhang Ming 
Published: August 2022

AbstractThis study aimed to investigate and compare the effects of two widely used nonfusion posterior dynamic stabilization (NPDS) devices, pedicle screw‐based dynamic stabilizer (PSDS) and interspinous process spacer (IPS), on biomechanics of the implanted lumbar spine under static and vibration loadings. The finite element model of healthy human lumbosacral segment was modified to incorporate NPDS device insertion at L4–L5 segment. Bioflex and DIAM were used as PSDS‐based and IPS‐based NPDS devices, respectively. As a comparison, lumbar interbody fusion with rigid stabilization was also simulated at L4–L5. For static loading, segmental range of motion (ROM) of the models under moments of four physiological motions was computed using hybrid testing protocol. For vibration loading, resonant modes and dynamic stress of the models under vertical excitation were extracted through random response analysis. The results showed that compared with the rigid fusion model, ROM of the nonfusion models was higher at L4–L5 level but lower at adjacent levels (L1– L2, L2–L3, L3–L4, L5–S1). Compared with the Bioflex model, the DIAM model produced higher ROM at L4–L5 level but lower ROM at adjacent levels, especially under lateral bending and axial rotation; resonant frequency of the DIAM model was slightly lower; dynamic response of nucleus stress at L4–L5 level was slightly higher for the DIAM model, and the dynamic stress at adjacent levels was no obvious difference between the nonfusion models. This study reveals biomechanical differences between the Bioflex and DIAM systems, which may provide references for selecting surgical approaches in clinical practice.
Dephosphorylation of Y228 and Y217 and phosphorylation of Y335 in p120 catenin activate convergent extension during zebrafish gastrulation
Authors: Shan Botao , Horton Emma C. , Xu Shan C. , Huntington Kelsey E. , Kawano Dane K. , Mendoza Clemence L. , Lin Laura , Stafford Christopher M. , Allen Emili D. , Huang Joyce , Nakahara Hiroko , Greenstein Lewis E. , Hille Merrill B. 
Published: August 2022

AbstractBackgroundThe cadherin‐associated protein p120 catenin regulates convergent extension through interactions with cadherin proteins, Cdc42, and Rac1, as we previously showed in zebrafish (Danio rerio). Phosphorylation of p120 catenin changes the nature of its activity in vitro but is virtually unexplored in embryos. We used our previously developed antisense RNA splice‐site morpholino targeted to endogenous p120 catenin‐δ1 to cause defects in axis elongation probing the functions of three p120 catenin tyrosine‐phosphorylation sites in gastrulating zebrafish embryos.ResultsThe morpholino‐induced defects were rescued by co‐injections with mouse p120 catenin‐δ1‐3A mRNAs mutated at residues Y228 and Y217 to a non‐phosphorylatable phenylalanine (F) or mutated at residue Y335 to a phosphomimetic glutamic acid (E). Co‐injection of the complementary mutations Y228E, Y217E, or Y335F mRNAs partially rescued embryos whereas dual mutation to Y228E‐Y217E blocked rescue. Immunopurification showed Y228F mutant proteins preferentially interacted with Rac1, potentially promoting cell migration. In contrast, the phosphomimetic Y228E preferentially interacted with E‐cadherin increasing adhesion. Both Y228F and Y335F strongly bind VAV2.Conclusionsp120 catenin serves dual roles during gastrulation of zebrafish. Phosphorylation and dephosphorylation of tyrosine residues Y217, Y228, and Y335 precisely balance cell adhesion and cell migration to facilitate somite compaction and axis elongation.
Seasonal climatic niche and migration movements of Double‐crested Cormorants
Authors: King D. Tommy , Wang Guiming , Cunningham Fred L. 
Published: August 2022

AbstractAvian migrants are challenged by seasonal adverse climatic conditions and energetic costs of long‐distance flying. Migratory birds may track or switch seasonal climatic niche between the breeding and non‐breeding grounds. Satellite tracking enables avian ecologists to investigate seasonal climatic niche and circannual movement patterns of migratory birds. The Double‐crested Cormorant (Nannopterum auritum, hereafter cormorant) wintering in the Gulf of Mexico (GOM) migrates to the Northern Great Plains and Great Lakes and is of economic importance because of its impacts on aquaculture. We tested the climatic niche switching hypothesis that cormorants would switch climatic niche between summer and winter because of substantial differences in climate between the non‐breeding grounds in the subtropical region and breeding grounds in the northern temperate region. The ordination analysis of climatic niche overlap indicated that cormorants had separate seasonal climatic niche consisting of seasonal mean monthly minimum and maximum temperature, seasonal mean monthly precipitation, and seasonal mean wind speed. Despite non‐overlapping summer and winter climatic niches, cormorants appeared to be subjected to similar wind speed between winter and summer habitats and were consistent with similar hourly flying speed between winter and summer. Therefore, substantial differences in temperature and precipitation may lead to the climatic niche switching of fish‐eating cormorants, a dietary specialist, between the breeding and non‐breeding grounds.