bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Feasibility Of Telemedicine In Implementation Of Therapeutic Hypothermia For Management Of Neonatal Hypoxic-Ischemic Encephalopathy In Resource-Limited Areas.
Authors: Hadid Adnan , AL-Shantout Taher S. , Terkawi Rayan S. , Aldbes Baraa M. , Zahran Manal M. , Alsatouf Fadia A. , Najjar Hani , Mughrabieh MHD Hassan , Alhadid Nour A. , Altirkawi Khalid 
Published: February 2022

Abstract
Background: Telemedicine is widely used in neonatal services in developed countries. Lack of expertise and/or facilities, however, limited its use in developing countries and around areas of military conflicts. To our knowledge, no reports are demonstrating the feasibility of administering therapeutic hypothermia (TH) through telemedicine to neonates with hypoxic-ischemic encephalopathy (HIE) in resource-limited areas.Methodology: This is a retrospective study, evaluating 22 patients who received TH, guided by telemedicine, through a mobile app (Telegram®). We assessed the feasibility of utilizing Telemedicine in guiding the application of TH to infants affected with HIE in the North-West of Syria between July 2020 and July 2021.Results: Out of 5,545 newborn infants delivered during the study period, 22 patients were eligible for TH guided by Telemedicine. Patients were referred for consultation at a median (IQR) of 137 (35-165) minutes of life. A median (IQR) of 12 (3-18) minutes elapsed between the call for a consultation and the consultant response, and a median (IQR) of 30 (0-42) minutes elapsed between seeking the consultation and the initiation of cooling therapy. Eighteen patients completed cooling for 72 hours. The patients' temperatures were within the target range (33-34°C) most of the time (84.1%).Conclusion: Telemedicine is a feasible method to guide the implementation TH for HIE in resource-limited areas. The short-term success rate is relatively high; however, further studies with a larger population are needed to confirm these findings.
Dietary powder and molecular imprinted polymer nanoencapsulated sodium propionate to enhance growth performance, digestive enzymes activity, antioxidant defense and mucosal immune response in African cichlid (Labidochromis lividus) fingerlings
Authors: sarkheil mehrdad , Safari Omid , Kordestani Davood 
Published: February 2022

Abstract

This study was conducted to examine the effects of powder sodium propionate (P-SP) and SP loaded molecular imprinted polymer (MIP) nanoparticles (MIP-SP NPs) on growth, skin mucosal immune parameters and digestive and liver enzymes activities of African cichlid (Labidochromis lividus) fingerlings. The synthetized MIP-SP particles were characterized via FE-SEM/EDS, TEM, FTIR and ICP-OES techniques. Fish with an average weight of 500±2 mg were stocked into 12 experimental units and fed with experimental diets prepared by supplementation of basal diet (control) with MIP NPs, P-SP (5 g SP Kg−1 of dry diet) and MIP-SP NPs for 8 weeks. The findings demonstrated that growth performance parameters improved in the MIP-SP NPs followed by the P-SP dietary group compared to the control groups (P<0.05). The activity of digestive enzymes of lipase, trypsin, protease and alkaline phosphatase was higher in the fish fed with SP-supplemented diets than the controls (P<0.05). The highest activity of protease and lipase enzymes was observed in the MIP-SP NPs dietary group (P<0.05). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of liver tissue decreased in the SP dietary groups, while the catalase (CAT), superoxide dismutase (SOD) and alkaline phosphatase (ALP) levels increased in comparison to the control groups (P<0.05). The highest SOD and ALP levels were observed in the fish fed with the MIP-SP NPs-supplemented diet (P<0.05). The skin mucosal immune indices including alternative haemolytic complement activity (ACH50), lysozyme and total immunoglobulin (Ig) levels increased in the MIP-SP NPs and P-SP dietary groups in comparison to the controls (P<0.05). The findings indicated that sodium propionate encapsulated in molecular imprinted polymer nanoparticles could enhance the efficiency of dietary SP in African cichlid fish.
Text Feature Extraction of Myopia-related Information on Social Media —A Study Based on the Text Sentiment and Statistical Evidence
Authors: Zhang Zuyue , Jiang Mengyao , Rao Qingmao , Wang Haolin , Ye Chunlv , Cheng Ting , Hou Xiaorong 
Published: February 2022

Abstract
Background: In recent years, health information has become easily available on in social media. However, most of the available information lacks a persuasive effect. Texts providing information in health science on social media may affect how readers are convinced of the evidence presented. The purpose of this study is to examine health information on myopia prevention in WeChat using text sentiment indicators and to assess the extent of use of statistical evidence.Methods: A Web crawler is employed to collect health science information data from the WeChat official account. A total of 4,645 myopia prevention texts of children in WeChat were collected, and 813 valid texts were included in the study. A paired-samples T-test was used in the study. The text sentiment and use of statistical evidence of health science information were extracted by analysing the text features.Results: Results show higher use of positive words as compared to negative words (P < 0.01). Statistical evidence was used in over 96% of the text. Suggestions for the development of health science information in terms of text sentiment and statistical evidence are also proposed.Conclusions: This study investigated the emotional features of text and use features of statistical evidence of health science information in WeChat. The study found that producers of information related to myopia prevention prefer positive emotions and use statistical evidence in most information.
Lentivirus-Mediated Small Interfering RNA Targeting Piezo1 Inhibits Cartilage Matrix degeneration in Osteoarthritis Chondrocytes
Authors: Liu Yikai , Lu Xinzhe , Zhang Zian , Li Tao , Zhang Haining 
Published: February 2022

Abstract
Osteoarthritis (OA) is triggered by abnormal distraction stress, which eventually leads to cartilage degeneration. However, it remains unclear how the mechanical stress factor acts an effect on the destruction of the cartilage matrix. As a kind of mechanical sensitive ion channel protein, Piezo1 has the ability to sense and transmit mechanical signals to cells. Previous studies have found that the stress sensitivity of Piezo1 is closely related to the directionality of mechanical stress and the presence of extracellular matrix. This research aimed to explore the role of lentivirus-mediated Piezo1-siRNA transfection in extracellular matrix in chondrocytes of cultured osteoarthritis in vitro and to investigate the effect of Piezo1 on the progression of osteoarthritis. In this study, we demonstrated that the expression of Piezo1 mRNA in transfection group was significantly decreased after 24h mechanical stress was applied. However, the expression trend of collagen II and aggrecan was opposite to that of Piezo1, matrix metalloproteinase-13 (MMP-13), and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5). Our results indicate that Piezo1 could be used as a possible therapeutic target to attenuate the destruction of cartilage matrix induced by mechanical stress. Lentiviral-mediated Piezo1-siRNA can effectively interfere with the expression of Piezo1 and protect the expression of the extracellular cartilage matrix.
The influence of gastrectomy for gastric cancer on the spontaneous disappearance of Helicobacter pylori: A single-center prospective study
Authors: Yanagawa Senichiro , Fujikuni Nobuaki , Tanabe Kazuaki , Nakahara Masahiro , Noriyuki Toshio 
Published: February 2022

Abstract
Background: Helicobacter pylori (HP) eradication is recommended after endoscopic treatment of early gastric cancer (EGC). However, although cases of spontaneous resolution of HP after gastrectomy due to changes in the environment have been reported, there is no evidence for the efficacy of HP eradication in suppressing atypical carcinogenesis after gastrectomy. Herein, we prospectively studied the spontaneous disappearance rate of HP after gastrectomy in patients with gastric cancer.Methods: From April 2016 to May 2020, 100 patients who underwent gastrectomy with preoperatively confirmed HP in the stool antigen test were tested one year after surgery in this trial. There were 20 cases of total gastrectomy (TG), 9 cases of proximal gastrectomy (PG), and 71 cases of distal gastrectomy (DG) consisting of 29 cases with Billroth-1 (B-1) reconstruction and 42 cases with Roux-en-Y (RY) reconstruction.Results: After one year, 46 patients were positive, and 54 patients were negative for HP infection. In the univariate analysis, surgical procedure, pathological stage, and the presence of postoperative chemotherapy were significant factors for spontaneous resolution of HP infection. In multivariate analysis, only the surgical technique of TG remained as an independent factor. There were no significant differences in the rates of HP resolution between B-1 and RY reconstruction.Conclusions: One year after gastrectomy, spontaneous disappearance of HP was observed in approximately half of the patients. Therefore, HP eradication in patients with gastric cancer in the early postoperative period is not necessary, even if they are preoperatively positive for HP infection.Trial registration: Registration number: University Hospital Medical Information Network (UMIN) Clinical Trials Registry (UMIN ID: UMIN000046614); Registration date: 12/01/2022, retrospectively registered.
Clinical implication and immunological landscape analyses of ANLN in pan-cancer, a new target for cancer research
Authors: Zhang Lan , Wei Yong , He Yan , Wang Xiaping , Huang Zebo , Sun Libing , Chen Jie , Zhu Qingyi , Zhou Xin 
Published: February 2022

Abstract
Background
Actin-binding protein anillin (ANLN) is mainly involved in the process of cytokinesis and known to be dysregulated in diverse cancers. However, the role of ANLN in pan-cancer prognosis and tumor immunity remains unclear.
Methods
Gene expression profiles of 31 solid tumors were fetched from The Cancer Genome Atlas (TCGA) database. ANLN mRNA and protein expression were quantified via quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). Protein expression of ANLN was further confirmed in Human Protein Atlas (HPA) database. Cox regression and Kaplan-Meier analysis were utilized to assess the prognostic value of ANLN in different tumors. The association between ANLN and different immune gene markers and infiltration cells were analyzed via ESTIMATE and CIBERSORT. A BLCA immunotherapy cohort: IMvigor (210) was utilized to confirm the role of ANLN in immune response.
Results
ANLN upregulation was detected in 21 types of cancers and was correlated with poor overall survival (OS), disease-free interval (DFI) and progression-free interval (PFI) in most cancers except in THYM. Additionally, correlation analysis revealed a significant association between ANLN expression and tumor mutation burden (TMB), microsatellite instability (MSI), immune cells infiltration and immune checkpoint genes in most cancer types. A BLCA immunotherapy cohort confirmed that patients with higher ANLN level had better immune responses and longer OS.
Conclusions
ANLN may serve as a prognostic biomarker for pan-cancer. ANLN upregulation is associated with higher TMB, MSI and immune cell infiltration in multiple types of tumors, shedding new light for cancer treatment.
Designing non-Hermitian real spectra
through electrostatics
Authors: Lee Ching Hua , Longhi Stefano , Li Linhu , Yang Russell , Tan Jun Wei , Tai Tommy Ying Zhe , Koh Jin Ming 
Published: February 2022

Abstract
Non-hermiticity presents a vast newly opened territory that harbors new physics and applications such as lasing and sensing. However, only non-Hermitian systems with real eigenenergies are
stable, and great efforts have been devoted in designing them through enforcing parity-time (PT) symmetry. In this work, we exploit a lesser-known dynamical mechanism for enforcing real spectra, and develop a comprehensive and versatile approach for designing new classes of parent Hamiltonians with real spectra. Our design approach is based on a novel electrostatics analogy for modified non-Hermitian bulk-boundary correspondence, where electrostatic charge corresponds to density of states and electric fields correspond to complex spectral flow. As such, Hamiltonians of any desired spectra and state localization profile can be reverse-engineered, particularly those without any guiding symmetry principles. By recasting the diagonalization of non-Hermitian Hamiltonians as a Poisson boundary value problem, our electrostatics analogy also transcends the gain/loss-induced compounding of floating-point errors in traditional numerical methods, thereby allowing access to far larger system sizes.
The Specific Inhibition of the Cardiac Electrogenic Sodium/Bicarbonate Cotransporter Leads to Cardiac Hypertrophy.
Authors: Mattía Romina Di , Diaz-Zegarra Leandro , Blanco Paula , Valverde Carlos , Gonano Luis , De Giusti Carolina Jaquenod , Portiansky Enrique , Vila-Petroff Martin , Aiello Ernesto , Orlowski Alejandro 
Published: February 2022

Abstract
Two alkalinizing mechanisms coexist in cardiac myocytes in order to maintain the intracellular pH: sodium/bicarbonate cotransporter (electroneutral isoform NBCn1 and electrogenic isoform NBCe1) and sodium/proton exchanger (NHE1). The dysfunction of these transporters has been previously reported as responsible for the development of cardiovascular diseases. The aim of this work was to evaluate the contribution of the downregulation of the NBCe1 to the development of cardiac hypertrophy. To specifically reduce NBCe1, we used shRNA cloned into a cardiotropic adeno-associated vector (AAV9-shNBCe1). After 28 days of being injected with AAV9-shNBCe1, the expression and the activity of NBCe1 in the rat heart were reduced. Strikingly, the downregulation of NBCe1 causes significant hypertrophic heart growth, lengthening of the action potential in isolated myocytes, increase in the duration of the QT interval and increase in the frequency of Ca2+ waves without any significant changes in Ca2+ transients. An increased compensatory expression of NBCn1 and NHE1 was also found. We conclude that the reduction of NBCe1 is sufficient to induce cardiac hypertrophy and modify electrical features of the rat heart.
Chronic necrotizing granulomatous skin lesions and MHC class I deficiency syndrome due to TAP2 deficiency
Authors: Darazam Ilad Alavi , Shahrooei Mohammad , Hakamifard Atousa , Olyaei Nasrin Alipour , Zerehpoosh Farahnaz Bidari , Gharehbagh Farid Javandoust , Hatami Firouze , Lotfollahi Legha , Mansouri Nahal , Casanova Jean-Laurent , Mansouri Davood 
Published: February 2022

Abstract
Major histocompatibility complexes class I (MHC- I) deficiency, also known as bare lymphocyte syndrome type 1 (BLS-1), is a rare autosomal recessively inherited immunodeficiency disorder with remarkable clinical and biological heterogeneity. Transporter associated with antigen processing (TAP) is a member of the ATP-binding cassette superfamily of transporters and consists of two subunits, TAP1 or TAP2. Any defect resulting from a mutation or deletion of these two subunits, adversely affects the peptide translocation in the endoplasmic reticulum, which is an important process for the proper assembly of MHC- I molecules. To date, few patients with reduced cell surface expression of MHC-I molecules have been reported. Herein; we described two Iranian cases with 2 and 3 decades delayed diagnosis of chronic necrotizing granulomatous skin lesions due to TAP2 deficiency without pulmonary involvement; and then segregation analysis in family members was performed by PCR and Sanger sequencing among 10 members of their family and found three homozygous mutation in three asymptomatic patients.
The trait coding rule in phenotype space
Authors: Wang Jianguo , He Xionglei 
Published: February 2022

Abstract
Is it possible to infer the genetically determined value of a quantitative trait from other traits? To answer the question we need to know how traits are coded in phenotype space (P) which can be partitioned into a subspace determined by genetic factors (PG) and a subspace affected by non-genetic factors (PNG). Evolutionary theory predicts PG is composed of limited dimensions while PNG may have infinite dimensions, which suggests a novel dimension decomposition method to separate them. After validating the method using simulation we applied it to a yeast phenotype space comprising 405 traits. The obtained yeast PG matches the actual genetic components of the yeast traits, explains the broad-sense heritability, and facilitates the mapping of quantitative trait loci, highlighting the success of the subspace separation. A limited number of latent dimensions in the PG were found to be recurrently used for coding the diverse yeast traits, while dimensions in the PNG tend to be trait specific and increase constantly with trait sampling. Similar results were obtained by analyzing the UK Biobank human brain phenome, which elucidated the genetic versus non-genetic origins of the left-right asymmetry of brain. In sum, phenotypic traits are coded by a rather small set of genetically determined basic dimensions and numerous trait-specific dimensions that are shaped by non-genetic factors, a rule enabling the identification of genetic components of quantitative traits based solely on phenotype.
Internet Gaming Disorder And Internet Addiction: Comparing Italian And Migrant Children And Adolescents
Authors: Valtolina Giulio , Boerchi Diego , Milani Luca 
Published: February 2022

Abstract
The DSM-5 has included Internet Gaming Disorder (IGD) as a potential new behavioral addiction related to some developmental risks such as depression, anxiety, attention disorders, social phobia, behavioral disorders, low academic performance. IGD stemmed from the previously proposed diagnosis of Internet Addiction (IA), which was also considered a behavioral addiction related to the Web's uncontrolled use and was correlated with maladaptive developmental outcomes. Literature shows a specific higher vulnerability of the migrant population to behavioral addictions – particularly gambling. Given this vulnerability and the amplitude of Italy's migration phenomenon, it is important to extend the knowledge about the risk of gaming and internet addiction to migrant adolescents and children in Italian schools. Thus, the aims of the present study are 1) to test the differences between Italian and migrant students in terms of problematic use of videogames and the Internet, and 2) to identify any risk factors predicting the emergence of problematic involvement in videogame activities and Internet use. We administered a survey to 532 students (64.5% females), aged between 9 and 20 years old (M = 15.80; DS = 2.16), attending secondary schools of first and second grade in 4 Italian cities. 9.7% of the students declared that they do not have Italian citizenship. The survey comprised specific instruments to measure Internet Gaming Disorder and Internet Addiction, respectively the VGA Questionnaire (VGA) and the Internet Addiction Test (IAT). Other instruments measured two areas of adaptation that literature highlighted as correlated to the two disorders: Coping strategies (assessed by the Children's Coping Strategies Checklist – Revised 1; CCSC-R1) and Interpersonal relations (measured by Interpersonal Relationship Test; TRI). Results indicate that the migrant condition can be a risk factor for IGD and IA, mainly when associated with avoidant coping strategies. Better interpersonal relations with female and male peers can be potential protective factors that counterbalance addiction risk. Implications point to fostering inclusion interventions aimed to migrant children to reduce the risk of gaming addiction in this specific group.
Comparative genomics of the human genome and six bat genomes using AI: Mb-level CpG and TFBS islands
Authors: Iwasaki Yuki , Ikemura Toshimichi , Wada Kennosuke , Wada Yoshiko , Abe Takashi 
Published: February 2022

Abstract
BackgroundEmerging infectious disease RNA viruses, such as the SARS-CoV-2 and Ebola viruses, are thought to rely on bats as their natural reservoir hosts. Since these zoonotic viruses pose a great threat to humans, it should be important to characterize the bat genome from multiple perspectives. Unsupervised artificial intelligence (AI) methods extracting novel information from big sequence data without prior knowledge or particular models are highly desirable for obtaining unexpected insights. We previously established a batch-learning self-organizing map (BLSOM) of the oligonucleotide composition that reveals novel genome characteristics from big sequence data.ResultsIn this study, using the oligonucleotide BLSOM, we conducted a comparative genome study of humans and bats. BLSOM is an explainable-type AI that reveals the diagnostic oligonucleotides contributing to sequence clustering (self-organization). When the unsupervised AI reveals unexpected and/or characteristic features, these features can be studied in more detail via the much simpler and more direct standard distribution map method. Based on this combined strategy, we identified the Mb-level enrichment of CG (Mb-level CpG islands) around the termini of bat long-scaffold sequences. In addition, a class of CG-containing oligonucleotides were enriched also in the centromeric and pericentromeric regions of human chromosomes. Oligonucleotides longer than tetranucleotides often represent binding motifs for a wide variety of proteins, e.g., transcription factor binding sequences (TFBSs). By analyzing penta- and hexanucleotide compositions, we observed the evident enrichment of a wide range of hexanucleotide TFBSs in centromeric and pericentromeric heterochromatin regions on all human chromosomes.ConclusionTFBSs which are enriched in centromere and pericentromeric heterochromatin regions, are thought to play an important role in the formation of nuclear 3D structures. Our AI-based analysis should help us to understand differential features of nuclear 3D structures of the human and bat genomes.