bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic and Immunological Role of FCER1G in multiple cancers
Authors: Zhang Xiaoxuan , Cai Jing , Yang Zhenzhou , Song Fangzhou 
Published: April 2022

Abstract
The high-affinity IgE receptor gamma subunit gene (FCER1G) plays a pivotal role in allergic inflammatory signaling, which is closely associated with allergic reactions. Previous studies have shown that FCER1G is an innate immunity gene and is involved in the development of many diseases. However, the role of FCER1G in pan-cancer has not been fully studied. This study aimed to explore the prognostic value of FCER1G in pan-cancer and investigate the relationship between FCER1G expression and immune infiltration. We analyzed FCER1G mRNA expression in the Oncomine, TIMER, and GEPIA databases. We used GEPIA, PrognoScan, and Kaplan-Meier Plotter to analyze the prognostic value of FCER1G. We explored the correlations between FCER1G expression and immune infiltration in TIMER databases. Cytoscape was used to perform pathway enrichment and functional enrichment analyses. Spearman method was used to determine the statistical significance.FCER1G can act as a prognostic marker for pan-cancer, FCER1G expression can affect patients' prognosis and correlate with immune cells infiltration. In kidney renal clear cell carcinoma and thyroid carcinoma, FCER1G is closely associated with different immune cells infiltration states and tumor purity, and immune infiltration can interact with FCER1G-mediated activities both in tumor cells and immune cells. FCER1G can be used to predict the efficacy of immunological checkpoint therapy among these types of tumors patients. Our study also provides an important basis for the clinical use of FCER1G to assess the patient immune status and the selection of individualized immunotherapy options.
Combined preoperative plasma fibrinogen and D-Dimer concentration as a prognostic marker of endometrial cancer
Authors: Wan Lixin , Wu Yuanyuan , Yao Xiaojuan , Han Jingru , Si Jiaoyang , Wei Xiajie , Yang Xiaotian , Wang Yang , Yu Guiqing , Liu Yangfan , Yang Zheng , Shan Minghui , Zheng Jiaojiao , Yang Hushan , Bao Dengke 
Published: April 2022

Abstract
Endometrial cancer (EC) is one of the most common gynecological malignancies. The aim of this study was to evaluate the combined prognostic value of D-dimer and fibrinogen in EC patients. We evaluated the prognostic value of preoperative plasma fibrinogen and D-Dimer concentration in EC patients using Kaplan-Meier curve analysis and Cox proportional hazards regression model. Kaplan-Meier curve analysis demonstrated that EC patients with higher levels of plasma fibrinogen or D-Dimer had a significantly poorer overall survival (OS) and recurrence-free survival (RFS). Furthermore, combination of fibrinogen and D-Dimer levels significantly improved the efficacy for predicting RFS of EC patient. Patients with higher fibrinogen or D-Dimer levels exhibited a significantly poorer RFS in both the univariate and multivariate analysis. In conclusions, the preoperative plasma fibrinogen and D-Dimer concentrations may serve as potential prognostic factors for EC patients, and combination of fibrinogen and D-Dimer concentration may be a potential marker for predicting RFS of EC patients.
Inducing Dzyaloshinskii-Moriya interaction in symmetrical multilayers using post annealing
Authors: ahmadi khadijeh , Mahfouzi Farzad , Jamilpanah Loghman , Mohseni Morteza , Böttcher Tobias , Pirro Philipp , Kioussis Nicholas , Åkerman Johan , Mohseni Seyed Majid 
Published: April 2022

Abstract
The interfacial Dzyaloshinskii-Moriya Interaction (iDMI) is an antisymmetric exchange interaction that is induced by the broken inversion symmetry at the interface of, e.g., a ferromagnet/heavy metal. Thus, the presence of iDMI is not expected in symmetrical multilayer stacks of such structures. Here, we use thermal annealing to induce the iDMI in a [Py/Pt]×10 symmetrical multilayer stack. Brillouin light scattering spectroscopy is used to directly evidence the iDMI induction in the annealed sample. Structural characterizations highlight the modified crystallinity as well as a higher surface roughness of the sample after annealing. First principles electronic structure calculations demonstrate a monotonic increase of the iDMI with the interfacial disorder due to the interdiffusion of atoms, depicting the possible origin of the induced iDMI. The presented method can be used to tune the iDMI strength in symmetric multilayers, which are the integral part of racetrack memories, magnonic devices as well as spin-orbitronic elements.
Antibiotic treatments to mothers during the perinatal period leaving hidden trouble on infants
Authors: Ji Chenyang , Zhang Geer , Xu Siyuan , Xiang Qingyi , Huang Meishuang , Zhao Meirong , Bai Xiaoxia 
Published: April 2022

Abstract
Antibiotic application during the perinatal period is unavoidable in clinical, but the potential effects on mothers and infants remain unknown. Herein, 25 breast milk samples from mothers who received cefuroxime (CXM) or CXM+cefoxitin (CFX) treatments and fecal samples from their infants were collected to investigate antibiotics induced undesirable effects on the microbiota of mothers and neonates. Furthermore, five fecal samples of infants, whose mothers had antibiotic treatments, were collected in a six-month postpartum follow-up visit to evaluate the long-term effects on infants’ gut microbiota. Moreover, the relative abundance of antibiotic resistance genes (ARGs) in fecal samples was compared to investigate the transfer of ARGs in infants’ gut microbiota. The results indicated that the antibiotic treatments had no influence on the microbiota of breast milk. The dominant bacteria phyla in the fecal samples had changed to Firmicutes and Proteobacteria after antibiotic treatments; while the bacterial community showed a recuperative trend in follow-up visits. Besides, the abundance of ARGs of infants’ gut microbiota demonstrated declining trend in CXM- and CXM+CFX-treated groups, while ARGs abundance presented a significant increasing trend after a 6-month recovery period.Conclusion: Antibiotic treatments to mothers during perinatal period would disturb the gut microbiota in neonates. The infants’ gut microbiota would partly return to their initial state after rehabilitation, but the transfer of ARGs would leave the hidden trouble of antibiotic resistance. Overall, data presented here can help to guide the scientific use of antibiotics during the perinatal period and provide potential approaches to mitigate the negative consequences.
Quantitative comparison of three-dimensional microstructure and determination of structural representative volume elements for the intact and compacted loess
Authors: Zhang Linxin , Qi Shengwen , Hou Xiaokun , Guo Songfeng , Li Zhiqing , Li Guoliang , Ma Lina 
Published: April 2022

Abstract
The pore structure is one of the most important properties of loess, which can directly affect the other properties such as water content, permeability and strength. It is of great significance to study the loess pore structure to ensure the safety and reliability of large-scale land creation and artificial infrastructure in the loess region. In this paper, the 3D pore characterization of intact loess and four kinds of compacted loess (with different dry densities) was investigated by microscale computed tomography. An efficient workflow is proposed to determine the structural representative volume elements of intact loess and compacted loess. Quantitative statistical analysis has shown that the heterogeneous level of the compacted loess is much greater than that of the intact loess on a microscopic scale. The intact loess has a higher porosity than the compacted loess, mainly because it has much more pores with an equivalent radius of 5~13μm. And the pores of intact loess are more connected and more stable. As the compactness increases, pores of the compacted loess have been compressed, separated, or even disappeared. And pore disappearance of the small pores (<7μm) mainly occurs under low compaction, while the compression and separation of medium (7~12μm) and large pores (>12μm) are dominant under high compaction. Moreover, the compacted loess will become more stable due to the present warm-humid climate.
B1 - and B1 /B2 -adrenergic receptor antagonists block 6-nitrodopamine- induced contractions on the rat isolated epididymal vas deferens
Authors: Lima Antonio Tiago , Amorim Amanda Consulin , Britto-Júnior José , Campitelli Raquel Rios , Fregonesi Adriano , Mónica Fabíola Z. , Antunes Edson , Nucci Gilberto 
Published: April 2022

Abstract
6-nitrodopamine (6-ND) is an endogenous modulator of the contractility in the rat isolated epididymal vas deferens (RIEVD) and considered to be the main peripheral mediator of the emission process. Use of selective and unselective b-adrenergic receptor antagonists have been associated with ejaculatory failure. Here, the effects of selective b1- and b1/ b2- adrenergic receptor antagonists on RIEVD contractions induced by 6-ND, dopamine, noradrenaline, adrenaline and EFS were investigated. The selective b1 adrenergic receptor antagonists atenolol (0.1 and 1mM), betaxolol (1mM) and metoprolol (1mM) and the unselective b1/b2- adrenergic receptor antagonists propranolol (1 and 10mM) and pindolol (10mM) caused significant rightward shifts of the concentration-response curve to 6-ND (pA2 6.41, 6.91, 6.75, 6.47 and 5.74; for atenolol, betaxolol, metoprolol, propranolol and pindolol), but had no effect on dopamine-, noradrenaline- and adrenaline-induced contractions. The effects of selective b1- and b1/ b2- adrenergic receptor antagonists at a higher concentration (atenolol 1mM, betaxolol 1mM, metoprolol 1mM, propranolol 10mM and pindolol 10mM) also reduced the EFS-induced RIEVD contractions in control but not in RIEVD obtained from L-NAME-treated animals. The selective b1- adrenoceptor agonist RO-363, the selective b2- adrenoceptor agonist salbutamol and the selective b3-adrenoceptor agonist mirabegron, up to 300 mM, had no effect on the RIEVD tone. The results demonstrate that b1- and b1-/ b2- adrenoceptor receptor antagonists act as 6-ND receptor antagonists in RIEVD, further confirming the main role of 6-ND in the RIEVD contractility.
Immunogenomic-based analysis of hierarchical clustering of diffuse large cell lymphoma
Authors: Wang Longhao , Yuan Wei , Li Lifeng , Shen Zhibo , Geng Qishun , Zheng Yuanyuan , Zhao Jie 
Published: April 2022

Abstract
Diffuse Large B cell lymphoma (DLBCL) is one of the most usual type of adult lymphoma with heterogeneousness in histological morphology, prognosis and clinical indications. Prior to this, several studies were carried out to determine the DLBCL subtype based on the analysis of the genome profile.However, classification based on assessment of genes related to the immune system has limited clinical significance for DLBCL. We systematically explored the DLBCL gene expression dataset and provided publicly available clinical information on patients with GEO and TCGA. In this research, 928 DLBCL samples from the Cancer Genome Atlas (TCGA) were applied, we calculated 29 immune-related genomes' enrichment levels in each sample and stratified them into high immunity that was based on ssGSEA score (Immunity_H, n=323,68.7%), moderate (Immunity_M, n= 135, 28.7%) and low (Immunity_L, n= 12,2.6%). The ESTIMATE algorithm was used to calculate matrix score (range: -1,800.51,901.99), immunity score (range: -1,476.28,780.33), estimated score (range: -2,618.28,098.14), and tumor purity (range:0.216 0.976), All of them were significantly correlated with immune subtypes (Kruskal Wallis test, P < 0.001). At the same time, the correlation of related genes was analyzed by immunohistochemistry staining. In addition, DLBCL cells were cultured in transfected and vitro with siRNA to verify correlation analysis and gene expression. Finally, human peripheral blood lymphocytes were incubated with DLBCL cells, and stained. Flow cytometry was applied to analyze genes' influence on immune function.By analysis, immune checkpoint and HLA gene expression levels were higher in the Immunity_H group (Kruskal Wallis test, P < 0.05). The levels of Tfhs (follicular helper T cells), Monocytes, CD8+ T cells, M1 Macrophages, M2 Macrophages, CD4+ memory activated T cells, were the most excellent in Immunity_H, and total survival rate was higher in the Immunity_L. The GO term discovered in Immunity_H is connected with immunity. Through analysis, IRF4 (MUM1) was identified by us as immunotherapeutic target and a potential prognostic marker for DLBCL, which was made sure by using molecular biology experimentations. To conclude, immunosignature made a connection between DLBCL subtypes play a position in DLBCL prognostic stratification. Immunocharacteristics-related DLBCL subtypes' construction predicts expected patient results and supplies conceivable immunotherapy candida.
A novel endoplasmic reticulum stress-related lncRNA prognostic risk model for skin cutaneous melanoma
Authors: li An-an , yan meiying 
Published: April 2022

Abstract
Objective
Endoplasmic reticulum stress(ERS) and Long non-coding RNAs (lncRNAs) play a important role in the occurrence and progression of melanoma. This study aimed to explore the prognostic impact of ERS-associated lncRNA profiles on cutaneous cutaneous melanoma (SKCM).
Methods
Get the raw date of SKCM from The Cancer Genome Atlas(TCGA). The ERS-related genes were obtained from the GSEA website, and then the ERS-related LncRNAs were obtained through the mRNA and LncRNA co-expression network. A prognostic risk model for the composition of ERS-related lncRNAs was identified by lasso regression analysis. Divided patients into high-risk and low-risk groups based on the model's risk score. Differences in survival outcomes, immune infiltration, chemotherapeutic drug sensitivity, and immune checkpoint gene expression were then assessed between the two groups.
Results
Thirty-nine ERS-related lncRNAs associated with prognosis were obtained. A prognostic risk model consisting of 10 ERS-related lncRNAs was identified. Patients in the low-risk group had a better prognosis than those in the high-risk group. Analysis of the tumor microenvironment revealed that risk scores correlated with infiltration of immune cells in 8. Patients in the low-risk group were more sensitive to the chemotherapy drugs paclitaxel and cisplatin than in the high-risk group.
Conclusion
This study identified a risk model composed of 10 ERS-related lncRNAs with significant prognostic value in SKCM and could provide guidance for clinical treatment.
Does L-carnitine have a protectiveeffect on the development of radiationinducedkidney damage in infancy?Scintigraphic and histopathologicevaluation
Authors: Cosar Rusen , Ozen Alaattin , Altun Gulay Durmus , Sut Necdet , Ibis Kamuran , Puyan Fulya Oz , Korkmaz Ulku , Parlar Sule , Nurlu Dilek , Ozler Talar 
Published: April 2022

Abstract
Background
Radiation-induced nephropathy (RIN) is an impairment of function caused by ionizing radiation which develops after6-12 months as acute or after years as chronic period.
Methods
This paper aims to clarify, whether L-carnitine has a protective effect in the prevention of RIN in an infant rat model.
Results
Two-week-old forty male Wistar albino rats were divided into four groups such as control (C), L-carnitine alone (LC), irradiation alone (RT), and L-Carnitine before irradiation (L-carnitine + RT), injected with LC (300 mg/kg) i.p., 30 minutes before the irradiation. The rats in the RT and L-Carnitine + RT groups were irradiated individually with a single dose of 8 Gy. All animals underwent both Tc99m DTPA dynamic renal imaging and Tc99m DMSA static renal imaging at the end of three months follow-up period. Proximal tubular degeneration, tubular atrophy, interstitial fibrosis, and glomerular degeneration were assessed histopathologically. While the kidney damage caused by irradiation was shown in line with both scintigraphy and histopathology findings, it was shown that L-carnitine did not have any negative effects on the kidney. Also, we could not demonstrate the protective effect of L-carnitine on radiation-induced kidney damage, both scintigraphically and histopathologically.
Conclusion
Although kidney damage due to irradiation was created in infantile rats successfully, the protective effect of L-carnitine could not be demonstrated in our study.
Immunoinformatics design of Bivalent Vaccine targeting S1-NTD and HA2 to simultaneously protect against SARS-CoV-2 and Influenza infections
Authors: Rezaei Marzieh , Sabzali Somaieh , Satari Mohammad 
Published: April 2022

Abstract
Introduction: Two of the most challenging viruses for vaccine development are SARS-COV-2 causing the current COVID-19 pandemic and influenza virus (H1N1) which spread annually causing seasonal epidemics or increase the pandemic risk. In this study, we analyzed the immonodominant epitope regions in Fusion peptides consisting of the Spike_S1_ N-terminal domain from SARS-COV-2 in-frame to hemagglutinin H2 (HA2) gene from Influenza A virus (H1N1) and also Human IFNɣ gene by two (G4S)3 linker. Method: The comprehensive analysis based on Immunoinformatic has been conducted on prediction servers to predict T and B cell epitopes. In silico cloning and expression in pET-28(+) expression vector and vaccine optimization were assessed. The overall model quality were accessed and the docking or binding affinity of designed vaccine to the Toll-like receptor 3 (TLR3) were analyzed. The efficiency of the constructed vaccine confirmed by appropriate expression of designed vaccine candidate tested by in silico cloning in pET–28(+) vector and codon optimization might increase the production of vaccine candidate into Escherichia coli strain k12.Result and discussion: In conclusion, we suggest that this fusion peptide would be an attractive design strategy toward developing bivalent vaccine against both COVID-19 and Influenza as promising vaccine candidate without need to reformulation or vaccination each year.
First Report of Avian Haemosporidians Infection and Associated Risk Factors in Red Jungle Fowl (Gallus gallus) in China
Authors: Li Zhao , Ren Xiao-Xia , Zhao Yin-jiao , Duan Bo-fang , Hu Na-ying , Zou Feng-Cai , Zhu Xing-Quan , He Jun-Jun , Liu Qi-Shuai 
Published: April 2022

Abstract
Background
Avian haemosporidians are critical hematocytozoon that infect both poultry and wild birds, responsible for anemia, acute tissue degeneration, and depopulation in wild birds. Poultry and wild birds have been reported as common reservoirs of hematocytozoon, but limited information was available for red jungle fowl (Gallus gallus) in China. The present study firstly investigated the prevalence and molecular characterization of haemosporidians in red jungle fowls.
Methods
Blood samples were collected from 234 red jungle fowls from Jinghong City of Yunnan province, and then the samples were stained and observed by using microscopy. The genomic DNA samples were extracted from these samples. The prevalence of avian haemosporidians was determined by the nested PCR targeting the mitochondrial cytochrome b (cytb) gene and staining morphology observation. Associated risk factors were analyzed by chi-square (χ2) test. Molecular characterization was investigated based on phylogenetic analysis of cytb sequence.
Results
Overall prevalence of avian haemosporidium was 74.8% (175/234), with Haemoproteus enucleator, Leucocytozoon californicus, Plasmodium juxtanucleare, and the prevalence of haemosporidians in adult fowls (81.1%, 107/132) was significantly higher (P = 0.012 < 0.05, df = 1, χ2 = 6.32) than juvenile gulls (66.7%, 68/102). Three representative novel lineages were revealed.
Conclusions
This study combined morphological and molecular phylogenetic analyses to identify avian haemosporidium in red jungle fowls, providing new information on the morphology, molecular epidemiology and geographical distribution of haemosporidian parasites. Our results suggested high prevalence and diverse genetic variations in these haemosporidians in fowls. To the best of our knowledge, this is the first records of haemosporidians infection in red jungle fowls in China.
How to More Awareness About Hip Dislocation, a unique case.
Authors: (MS MRCS) *Dr. Dheeraj Makkar , (MS) Prof. Ravi Sauhta 
Published: April 2022

Abstract
The hip is a stable ball-and-socket joint that can tolerate considerable mechanical strain. The anterior hip dislocation is the least common type of dislocation, accounting for around 8–15% of total hip dislocations. Bilateral anterior are even rarer and account for less than 1% of all dislocations. We report a unique case of a bilateral anterior dislocation that presented to our institute within 6 hours of trauma and was reduced immediately in an operation theatre under propofol anaesthesia. Hip dislocations constitute a medical emergency and should be detected clinically as soon as possible, confirmed radiographically, and treated quickly. If the anterior dislocation is treated promptly, the outcome is favourable. Follow-up X-rays are recommended every six months for the next two years following reduction to rule out any late problems.
In-hospital mortality of COVID-19 patients hospitalized with ST-segment elevation myocardial infarction: A meta-analysis
Authors: Cheema Huzaifa Ahmad , Ehsan Muhammad , Ayyan Muhammad , Shahid Abia , Farooq Minaam , Javed Muhammad Usman , Lak Hassan Mehmood , Ahmad Malik Qistas , Virk Hafeez Ul Hassan , Lakhter Vladimir 
Published: April 2022

Abstract
BackgroundPrevious meta-analyses have focused on investigating the impact of the Coronavirus Disease 2019 (COVID-19) pandemic on outcomes in STEMI patients. We aimed to examine the outcomes and prognosis following ST-segment elevation myocardial infarction (STEMI) among those with COVID-19 compared with those without COVID-19.MethodsPubMed and Embase were searched from inception till November 2021. We included only those studies that compared our primary outcome, in-hospital mortality, between COVID-19-positive and COVID-19-negative cohorts with primarily out-of-hospital STEMI. We conducted a random-effects meta-analysis to investigate the association between COVID-19 infection and mortality as well as other clinical outcomes.ResultsA total of 11 observational studies were included in our meta-analysis. Most of the studies were of sufficiently high quality. Our findings show that a diagnosis of COVID-19 in STEMI patients is associated with a large increase in mortality (OR 4.07; 95% CI: 2.48-6.69) and poorer clinical outcomes but with no increase in door-to-balloon (D2B) time (MD 9.45 minutes, 95% CI: -1.25 to 20.15 minutes).ConclusionsIn this meta-analysis, a diagnosis of COVID-19 was found to greatly increase the risk of mortality. An early assessment of COVID-19 status in STEMI patients is needed followed by urgent management.