bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of Salicylic acid and Silicon on Seed Yield, Oil Content, and Fatty Acids Composition in Safflower under Salinity Stress
Authors: Jam Bahareh Jamshidi , Shekari Farid , Andalibi Babak , Fotovat Reza , Jafarian Vahab , Dolatabadiyan Aria 
Published: November 2022

Abstract

Soil and water salinization has global impact on crop production and food security. Application of phytohormones and nutrients management is major approaches to reduce salinity risks. The effects of salicylic acid (0, 600, 1200, and 1800 μM) and silicon (0, 1.5, and 2.5 mM) foliar application on safflower seed yield and quality was investigated under salt stress conditions (1.7, 7.5, and 15 dS m-1). Salinity decreased capitulum number, seed number per capitulum, 100-seed weight, seed yield, oil percentage, oil yield, linoleic acid content, palmitic and linoleic acids yield, and seed potassium content. Application of salicylic acid (SA) and silicon (Si) increased biological yield, seed yield, oil content, oil yield, linoleic acid content, palmitic and linoleic acid yield but decreased stearic and oleic acid content and oleic acid yield. Harvest index was decreased with increasing salinity level, which indicates a stronger effect of salinity on seed yield rather than biomass production. In contrast, SA and Si, whether alone or together increased HI. The appropriate concentration of Si was different in salinity levels. Under non-stress and moderate stress conditions 2.5 mM Si showed better performance, while at severe salinity level, 1.5 mM Si showed a suitable state. Oil content and quality improved by increasing linoleic acid and reducing stearic and palmitic acids by application of SA and Si. Results suggest that the application of 1200 μM SA along with either levels of Si was more effective in improving quantitative and qualitative yield, especially under salinity stress conditions.
Estimating of Gases Emission from Dumping Waste Sites to Generating Energy; Case Study: Al-Hillah City, Iraq
Authors: Chabuk Ali , Jahad Udai A. , Majdi Ali , Majdi Hasan SH. , Isam Mubeen , Al-Ansari Nadhir , Laue Jan , Al-Ansari Nadhir 
Published: November 2022

AbstractMethane (CH4), and carbon dioxide (CO2) are only a few of the greenhouse gases that are significantly produced by humans and are found in landfills. This study presents an application of the Land GEM model to determine the environmental loads of dumping waste sites gases released from the landfill in Al-Hillah city, Iraq. The expected quantity of waste for the period (2023–2070) was used to calculate the environmental loads of dumping site gases. The quantified emission findings from loads of dumping site gases were contrasted with those from the model's default. The estimated landfill methane emissions from 2023 to 2070 total 874,904 tons of CH4, or an average of 18,227 tons of CH4each year. The findings of the gas emissions demonstrated that local elements significantly influenced the output of loads of landfill gases. The anticipated loads of total landfill gases, methane, and carbon dioxide can be utilized as supporting data for policies on landfill-derived renewable energy. the results showed that methane gas can produce the average value of electricity power of 287,442 Mw/year from 2023 to 2070. This process will reduce the environmental problems brought on by loads of landfill gas emissions and get rid of cumulative solid waste.
SUMOylation of RNF146 results in Axin degradation and activation of Wnt/β-catenin signaling to promote the progression of hepatocellular carcinoma
Authors: Yin Dong , Li Wenjia , Han Qingfang , Zhu Yuanxin , Zhou Yingshi , Zhang Jingyuan , Wu Weijun , Li Yu , Liu Long , Qiu Yuntan , Hu Kaishun 
Published: November 2022

Abstract
Aberrant SUMOylation contributes to the progression of hepatocellular carcinoma (HCC), yet the molecular mechanisms have not been well elucidated. RNF146 is a key regulator of the Wnt/β-catenin signaling pathway, which is frequently hyperactivated in HCC. Here, it is identified that RNF146 can be modified by SUMO3. By mutating all lysines in RNF146, we found that K19, K61, K174 and K175 are the major sites for SUMOylation. UBC9/PIAS3/MMS21 and SENP1/2/6 mediated the conjugation and deconjugation of SUMO3, respectively. Furthermore, SUMOylation of RNF146 promoted its nuclear localization, while deSUMOylation induced its cytoplasmic localization. Importantly, SUMOylation promotes the association of RNF146 with Axin to accelerate the ubiquitination and degradation of Axin. Intriguingly, only UBC9/PIAS3 and SENP1 can act at K19/K175 in RNF146 and affect its role in regulating the stability of Axin. In addition, inhibiting RNF146 SUMOylation suppressed the progression of HCC both in vitro and in vivo. And, patients with higher expression of RNF146 and UBC9 have the worst prognosis. Taken together, we conclude that RNF146 SUMOylation at K19/K175 promotes its association with Axin and accelerates Axin degradation, thereby enhancing β-catenin signaling and contributing to cancer progression. Our findings reveal that RNF146 SUMOylation is a potential therapeutic target in HCC.
Risk Factors Associated with Pneumocystis jirovecii Pneumonia in non-HIV Immunocompromised Patients and Co-pathogens Analysis by Metagenomic Next-Generation Sequencing
Authors: Huang Liping , Xu Shuyun , Huang Zhimin , Chen Yusheng , Xu Nengluan , Xie Baosong 
Published: November 2022

Abstract
Background: Pneumocystis jirovecii pneumonia (PJP) is one of the most common opportunistic infections in immunocompromised patients. However, the accurate prediction of the development of PJP in non-HIV immunocompromised patients is still unclear.
Methods: Non-HIV immunocompromised patients confirmed diagnosis of PJP by the clinical symptoms, chest computed tomography and etiological results of metagenomic next-generation sequencing (mNGS) were enrolled as observation group. Another group of matched non-HIV immunocompromised patients with non-PJP pneumonia were enrolled to control group. The risk factors for the development of PJP and the co-pathogens in the bronchoalveolar lavage fluid (BALF) detected by mNGS were analyzed.
Results: A total of 67 (33 PJP, 34 non-PJP) participants were enrolled from Fujian Provincial Hospital. The ages, males and underlying illnesses were not significantly different between the two groups. Compared to non-PJP patients, PJP patients were more tends to have the symptoms of fever and dyspnea. The LYM and ALB were significantly lower in PJP patients than in non-PJP patients. Conversely, LDH and serum BDG in PJP patients were significantly higher than in non-PJP controls. For immunological indicators, the levels of immunoglobulin A, G, M and complement C3, C4, the numbers of T, B, and NK cells, had no statistical difference between these two groups. Logistic multivariate analysis showed that concomitant use of corticosteroids and immunosuppressant (OR 14.146, P=0.004) and the lymphocyte counts < 0.7×109/L (OR 6.882, P=0.011) were risk factors for the development of PJP in non-HIV immunocompromised patients. 81.82% (27/33) and 64.71% (22/34) mixed infections were identified by mNGS in the PJP group and non-PJP group separately. CMV, EBV and Candida were the leading co-pathogens in PJP patients. The case numbers of CMV and EBV identified by mNGS in PJP group were significantly higher than that in the control group(p<0.005).
Conclusions: Clinicians should pay close attention to the development of PJP in non-HIV immunocompromised patients who possess the risk factors of concomitant use of corticosteroids and immunosuppressant and the lymphocyte counts < 0.7×109/L. Prophylaxis for PJP cannot rely solely on CD4+ T counts in non-HIV immunocompromised patients. Whether CMV infection increases the risk of PJP remains to be further investigated.
Effect of Grandivittin from Ferulago trifida Boiss. on the proliferation and apoptosis of human lung cancer A549 cells
Authors: Anbaji Fatemeh Zomorodi , Zargar Seyed Jalal , Tavakoli Saeed 
Published: November 2022

Abstract
Lung cancer is one of the deadliest cancers in the world. Introducing new promising agents can help the chemotherapeutic management of cancer. In the knowledge of oncology, plants are of special interest as a rich source of new antineoplastic and chemotherapeutic agents. Grandivittin (GRA) is one of the main constituents of Fenzl (Ferulago trifida Boiss.) with established medicinal, phytochemical, and pharmacological properties. This study aimed to evaluate the antineoplastic potential of GAR and its underlying mechanisms in human lung cancer A549 cells. The viability of the A549 cells after being treated with different concentrations of GRA for three following days was measured using the MTT method. The early and late apoptosis were assessed by fluorescence‐activated cell sorter analysis through annexin V/PI staining. The expression of apoptotic agents' genes (caspase3, caspase 9, Bcl2, Bax, and P53 ) was evaluated by the RT-PCR method. The GRA increased apoptotic cells and decreased cell viability in a dose- and time-dependent manner, in which only 50% of cells survived at a dose of 0.7 mM. The expression of Bax, P53, Caspase3, and caspase 9 genes in the A549 cells was significantly up-regulated after GRA treatment compared to control cells (P<0.05). On the other hand, the Bcl2 was significantly down-regulated after GRA treatment (P<0.05). The results indicated that the GRA can activate cell death in A549 lung carcinoma cells by ‎inducing both DNA toxicity p53 and cascade-dependent pathways. Therefore, the GRA may be a potential new therapeutic agent for the treatment of lung cancer.
WITHDRAWN: Using Ground Magnetic Data for Edge Basic Dykes Detection, Their Structures and Tectonics, in North Ras Millan Area, Southern Sinai, Egypt
Authors: Youssef Mohamed A. S. , Ata Ahmed S. A. Abu El 
Published: November 2022

Abstract

The main aim of this search is delineated the basaltic dykes edges, as a part of the structural-tectonic inferences of North Ras Millan (NRM) area, Southern Sinai, Egypt. A ground magnetic survey was conducted, to help edge delineation of the subsurface magnetized bodies. The magnetic data analysis is based on the filters of horizontal and vertical gradients, in which the edge detection is dependent on the tilt derivative (of the horizontal and vertical derivatives) and the tilt angle of the available ground magnetic data of the concerned area. This study applied a new edge-detection filter, called the enhanced total horizontal derivative of the tilt angle (ETHDR). ETHDR is the total horizontal derivative of the ratio of the first vertical derivative to the total horizontal derivative of the first order analytical signal amplitude. This paper compares the results of the analytical signal (AS), first vertical derivative (FVD), total horizontal derivative (THDR), tilt angle (TA), horizontal tilt angle and Enhanced total horizontal derivative of the tilt angle (ETHDR). The compared applied derivatives-based filters and the enhanced total horizontal derivative of the tilt angle produce more detailed results for the shallower and deeper magnetized structures, and gives sharp edges of the basic magnetized dykes, that surrounded by basement rocks of acidic nature. Structurally, the considered area is implicated by two sets of normal faults, older Pre-Miocene normal faults of NW-SE trend (Red Sea system) and younger Miocene oblique faults of NE-SW trend (Gulf of Aqaba system) as shown from Fig. (14), the younger NE-SW faults crossed the older NW-SE faults and displaced their step-like fault blocks in right and left lateral movements of strike sense. Also, the inferred basic dykes are generated generally through their intersections and particularly through the NE-SW younger set. Tectonically, these fault systems were created, due to the NNW-SSE Alpine stress of the early Mesozoic time, in the form of NW-SE (Gulf of Suez) and NE-SW (Gulf of Aqaba) fault systems of first order oblique faults of normal slip component during the pre-Miocene, followed by the shear slip component of the Miocene and Post-Miocene times, as expressed by the tectonic model of north Eastern Egypt during the Pre-Miocene, Miocene and Post-Miocene, Abu El-Ata and Helal, (1992)
Design Optimization of Coal Plant Construction Equipment Pipeline Based on BIM Technology
Authors: Chen Yu , Zhang Xiaohui 
Published: November 2022

Abstract
The coal plant is a project with large concrete volume and complex structure. There are various mechanical equipment inside it, and there are many conflicts in the pipeline.In order to improve the construction efficiency and quality of industrial buildings such as coal plant, and avoid technical problems such as collision between building structures and various equipment pipelines or omission of reserved holes, BIM technology is applied to the whole process design and construction of coal plant.The construction of actual projects is guided by such technical methods as model building, visual analysis of building interior space, collision detection and optimization in equipment pipeline design, which not only greatly improves the project quality, improves the construction efficiency, saves construction costs, but also better helps the rapid integration of industrialization and informatization of the coal industry.
Establishing an imaging prediction model to predict the remaining life of knee joint for varus knee osteoarthritis patients
Authors: Ma Wenru , Li Chunpu , Liu Guangzhen , Yu Tengbo , Zhang Yi 
Published: November 2022

Abstract
Purpose

This study aimed to establish a prediction model basing on onset age, gender, body mass index (BMI) and imaging measurements to predict the remaining life of knee joint for varus knee osteoarthritis patients, and to aid in patient selection and decision-making efforts regarding knee joint preservation and total knee arthroplasty (TKA).

Methods

Using data collected from our retrospective TKA database, 132 patients with varus knee osteoarthritis (153 knees) were included in the study. We focused on patients with a diagnosis of Kellgren–Lawrence (K-L) grade II in the initial stage of knee osteoarthritis (OA) accompanied by mild symptoms and a diagnosis of K-L grade IV with severe symptoms and TKA records at the endpoint. The interval between baseline and endpoint was defined as the remaining life of knee joint. The following imaging indexes of knee joints were assessed using standard anteroposterior (AP) radiography, medial proximal tibial angle (MPTA) and joint line convergence angle (JLCA) at baseline (MPTA0 and JLCA0) and at TKA period (MPTAT and JLCAT); changes in MPTA and JLCA between baseline and TKA period (ΔMPTA and ΔJLCA); and rates of change in MPTA and JLCA between baseline and TKA period (kMPTA and kJLCA). Meanwhile, basic information of patients was collected, including onset age, gender, body mass index (BMI). Multivariate linear regression analysis was performed for the prediction model about the remaining life of knee joint using statistically significant indexes.

Results

Multivariate linear regression analysis showed that, onset age, BMI, JLCA0, kMPTA and kJLCA were significantly negatively correlated with the remaining life of knee joint, which showed effectiveness in prediction model for TKA. Prediction model: remaining life of knee joint = 195.3–0.6×Onset age-2.0×BMI-4.4×JLCA0-159.9×kMPTA-205.5×kJLCA.

Conclusions

Onset age, BMI, JLCA0 and rates of change in MPTA and JLCA robustly predict the risk of requiring TKA.
Level of Evidence: Retrospective Study Level III
Cr2O3 doping effect on silica glass cooling rate
Authors: Eronyan Mikhail A. , Parfenov Peter S. , Kulesh Alexey Yu. , Meshkovskiy Igor K. , Untilov Alexander A. 
Published: November 2022

Abstract
In this paper we present the results on the effect of the SiO2 melt cooling rate on the morphology of its surface. The method of atomic force microscopy explores a surface roughness of the silica glass cooled at different rates after its heating up to more than 2100 оС. The study compares three modes of cooling glass rods with a diameter of 14 mm: convective heat exchange with the surrounding air, quenching with dropping the sample into a vessel with water, and radiation of silica glass doped with 0.1 % Cr2O3. The surface roughness of silica glass in the second and third modes of cooling was significantly less than in the conditions of convective heat exchange. Doping the silica glass with Cr2O3 increases the heat exchange coefficient by 2 times and decreases the surface roughness. The results of the research can contribute to improving the mechanical and optical properties of silica glass light guides.
Prioritising and fitting preventive vision care into existing health service utilization among older adults: a qualitative study
Authors: Lau Wing Yan , Liao Qiuyan , Lian Jinxiao , Yap Maurice 
Published: November 2022

Abstract
Background
Visual decline is inevitable in older adults. Utilizing preventive vision care can mitigate major risk factors, yet the uptake rate remains suboptimal globally. Barriers to seek preventive eye care were identified in the literature. However, the understanding of these barriers was limited by the quantitative approach, hindering the opportunity to suggest specific strategies to improve service uptake. This qualitative aimed to identify the factors influencing the decision-making process of older adults to use preventive vision care.

Methods
This was a qualitative study guided by the grounded theory approach. Purposive sampling was used to recruit the subjects. Semi-structured interviews were conducted with 25 community-dwelling adults aged 65 years or above in Hong Kong. Data were transcribed and constant comparison techniques were used for data analysis.

Results
The core theme revealed a decision-making process of being difficult in “prioritising and fitting preventive vision care into the existing health service utilization” for older people when deciding the utilization of preventive vision care. Five sub-themes were identified to explain the low preventive vision care utilization. Specifically, in absence of past preventive vision care utilization experience, most rely on other “previous health service utilization” to guide their judgment. “Low perceived preventive vision care needs” were due to perceived low disruption of symptoms in daily practices and lack of social cues. “Low perceived self-efficacy of service utilization” was due to limited information on service availability, limited social support, limited health literacy, and financial concerns. Unfulfilled “service expectation” also lowered their motivation to use preventive vision care.

Discussion
This study found that multiple factors influenced the decision-making process and reduced the motivation of subjects to use preventive vision care. Future interventions could exploit the benefit of social influences on the identified factors so that preventive vision care can smoothly fit into the ongoing healthcare use. Adopting a longitudinal qualitative perspective would enrich understanding the reasons for not using preventive vision care.
Investigating the expression of LNCRNAs TMPO-AS1, DDX11-AS1, and POLE gene in head and neck squamous cell carcinoma
Authors: Mokhtarinejad Mahnoosh , Pirhoushiaran Maryam Pirhoushiaran , Hesami Sara , Mehrabi Saman , Shirkoohi Reza , Lotfi Maryam , Farahani Abbas Shakoori 
Published: November 2022

Abstract
Background and aim: Head and neck cancer includes all cancers located in the head and neck area, including larynx, oral cavity, nasal cavities, lips, myopic sinuses, tongue and salivary glands. This study was conducted with the aim of evaluating the expression levels of LNCRNAs TMPO-AS1, DDX11-AS1 and POLE mRNA in tumor and normal tissues adjacent to the tumor of patients with HNSCC.
Methods and Materials: 50 fresh frozen samples were collected from patients with HNSCC. The expression levels of LNCRNAs TMPO-AS1, DDX11-AS1 and POLE mRNA were measured using real time PCR technique.
Results:Based on the findings of this study, an increase in the expression levels of DDX11-AS1 and POLE was observed in tumor tissues compared to the normal tissue adjacent to the tumor (P-value=0.947 and P-value= 0.997). From the point of view of examining the TMPO-AS1 gene, a decrease in expression (P-value=0.163) was observed in tumor samples compared to normal samples adjacent to the tumor. Also, a significant expression correlation (P-value=0.006) was observed between TMPO-AS1 and POLE in normal samples adjacent to the tumor. In addition, a significant expression correlation (P-value=0.001) was observed between DDX11-AS1 and POLE in normal samples adjacent to the tumor.
Conclusion:Therefore, DDX11-AS1 and POLE genes probably play a role as oncogenes in HNSCC, while TMPO-AS1 gene is considered as a tumor suppressor gene in this cancer.
Methyl ferulic acid alleviates neuropathic pain by inhibiting Nox4-induced ferroptosis in dorsal root ganglia neurons in rats
Authors: Liu Tielong , Wang Ruixue , Qi Wenqiang , Jia Lei , Ma Ketao , Si Junqiang , Yin Jieting , Zhao Yujia , Dai Zhigang , yin jiangwen 
Published: November 2022

Abstract
Neuropathic pain is a disease that has become one of the major public health problems and a global burden. Nox4-induced oxidative stress can lead to ferroptosis and neuropathic pain. Methyl ferulic acid (MFA) can inhibit the Nox4-induced oxidative stress. This study aimed to estimate whether methyl ferulic acid alleviates neuropathic pain by inhibiting the expression of Nox4 and its induction of ferroptosis. Adult male Sprague‒Dawley rats were subjected to spared nerve injury (SNI) model to induce neuropathic pain. After the establishment of the model, methyl ferulic acid was given 14 days by gavage. Nox4 overexpression was induced by microinjection of the AAV-Nox4 RNAi vector. All groups measured paw mechanical withdrawal threshold (PMWT), paw thermal withdrawal latency (PTWL), and paw withdrawal cold duration (PWCD). The expression of Nox4, ACSL4, GPX4, and ROS was investigated by Western blot and immunofluorescence staining. The changes in iron content were detected by a tissue iron kit. The morphological changes in mitochondria were observed by transmission electron microscopy. In the SNI group, the paw mechanical withdrawal threshold, the paw withdrawal cold duration decreased, the paw thermal withdrawal latency did not change, the Nox4, ACSL4, ROS, and iron content increased, the GPX4 decreased, and the number of abnormal mitochondria increased. Methyl ferulic acid can increase PMWT and PWCD but does not affect PTWL. Methyl ferulic acid decreased Nox4, ACSL4, and ROS levels, and iron content increased GPX4 expression and decreased the number of abnormal mitochondria. By overexpressing Nox4, the PMWT, PWCD, and ferroptosis of rats were more severe than those of the SNI group, but they could be reversed after treatment with methyl ferulic acid. Methyl ferulic acid can alleviate neuropathic pain, which is related to Nox4-induced ferroptosis.