bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stage-specific regulation of signalling pathways to differentiate pluripotent stem cells to cardiomyocytes with ventricular lineage
Authors: Satthenapalli Ramakanth , Lee Scott , Bellae Papannarao Jayanthi , Hore Timothy A. , Chakraborty Akash , Jones Peter P. , Lamberts Regis R. , Katare Rajesh 
Published: May 2022

Abstract
Background
Pluripotent stem cells (PSCs) can be an ideal source of differentiation of cardiomyocytes in vitro and during transplantation to induce cardiac regeneration. However, differentiation of PSCs into a heterogeneous population is associated with an increased incidence of arrhythmia following transplantation. We aimed to design a protocol to drive PSCs to a ventricular lineage by regulating Wnt and retinoic acid (RA) signalling pathways.

Methods
Mouse embryonic stem cells were cultured either in monolayers or three-dimensional hanging drop method to form embryonic bodies (EBs) and exposed to different treatments acting on Wnt and retinoic acid signalling. Samples were collected at different time points to analyse cardiomyocyte-specific markers by RT-PCR, flow cytometry and immunofluorescence.

Results
Treatment of monolayer and EBs with Wnt and RA signalling pathways and ascorbic acid, as a cardiac programming enhancer, resulted in the formation of an immature non-contractile cardiac population that expressed many of the putative markers of cardiac differentiation. The population exhibited upregulation of ventricular specific markers while suppressing the expression of pro-atrial and pro-sinoatrial markers. Differentiation of EBs resulted in early foetal like non-contractile ventricular cardiomyocytes with an inherent propensity to contract when stimulated.

Conclusion
Our results provide the first evidence of in vitro differentiation that mimics the embryonic morphogenesis towards ventricular specific cardiomyocytes through regulation of Wnt and RA signalling pathways.
European public health best practice portal - process and criteria for best practice assessment
Authors: Stepien Magdalena , Keller Ingrid , Takki Marianne , Caldeira Sandra 
Published: May 2022

Abstract
Background
Non-communicable diseases (NCDs) are major and growing burden on population health and the use and cost of healthcare in EU Member States and beyond. Different countries face many common challenges in public health and can learn from each other. The exchange of ‘best practices’ is one way to tackle the observed disparities in health sector. To address the United Nations Sustainable Development Goals, the European Commission developed the EU Public Health Best Practice Portal to facilitate the exchange of best practices and facilitate their implementation in other EU countries or regions. The ultimate aim of the portal is to reduce NCDs burden and the prevalence of their risk factors by promoting implementation and scale up of evidence-based effective interventions in the areas of health promotion, disease prevention and management of NCDs.

Results
This article presents the rationale and the process, ranging from best practice assessment to their transfer to interested Member States, applied in the EU Public Health Best Practice Portal. The portal selects best practices using rigorously defined criteria for best practice assessment. This article further provides an overview of other similar initiatives in Europe and internationally that collect and disseminate information on interventions and actions to combat NCDs.

Conclusion
Exchange of best practices is a promising tool in tackling NCDs. Transfer and scaling up of policies and interventions between countries may contribute to tackle disparities observed between countries in regards to the prevalence of risk factors and associated diseases.
A case series of group-based ketamine-assisted psychotherapy for patients in residential treatment for eating disorders with comorbid depression and anxiety disorders
Authors: Robison Reid , Lafrance Adele , Brendle Madeline , Smith Michelle , Moore Claire , Ahuja Sachin , Richards Scott , Hawkins Nicole , Strahan Erin 
Published: May 2022

Abstract
Background
Depression and anxiety outcome measures, safety/tolerability, patient satisfaction, and ease of implementation of group-based ketamine-assisted psychotherapy (G-KAP) delivered to patients in intensive residential eating disorder (ED) treatment were assessed.

Case presentation
This study reports on five participants with a diagnosis of an ED and comorbid mood and anxiety disorders who received weekly intramuscular ketamine injections in a group setting over 4 weeks. Measures of anxiety (GAD-7) and depression (PHQ-9) were administered pre-dose, 4-h post-dose, and 24-h post dose. Four of the 5 participants experienced clinically significant improvements on the PHQ-9 score (i.e., change greater than 5) while 2 of the 5 participants experienced clinically significant improvements on the GAD-7 score (i.e., change greater than 4) from pre-dose to 24-h post-dose after the last ketamine session. Dosing sessions were well tolerated, and no serious adverse events were reported. Clinical observations and participant reports corroborated improvements in depression and anxiety symptoms, good tolerability of ketamine treatment, and practical implementation of the G-KAP protocol in a residential ED treatment center.

Conclusions
This study suggests the potential utility of G-KAP as an adjunct to intensive, specialized ED treatment. Overall, this novel, cross-diagnostic intervention warrants future research to further explore its appropriateness in a treatment setting.
Machine Learning to Predict Risk of Relapse Using Cytologic Image Markers in Patients With Acute Myeloid Leukemia Posthematopoietic Cell Transplantation
Authors: Arabyarmohammadi Sara , Leo Patrick , Viswanathan Vidya Sankar , Janowczyk Andrew , Corredor German , Fu Pingfu , Meyerson Howard , Metheny Leland , Madabhushi Anant 
Published: May 2022

PURPOSE Allogenic hematopoietic stem-cell transplant (HCT) is a curative therapy for acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). Relapse post-HCT is the most common cause of treatment failure and is associated with a poor prognosis. Pathologist-based visual assessment of aspirate images and the manual myeloblast counting have shown to be predictive of relapse post-HCT. However, this approach is time-intensive and subjective. The premise of this study was to explore whether computer-extracted morphology and texture features from myeloblasts' chromatin patterns could help predict relapse and prognosticate relapse-free survival (RFS) after HCT. MATERIALS AND METHODS In this study, Wright-Giemsa–stained post-HCT aspirate images were collected from 92 patients with AML/MDS who were randomly assigned into a training set ( S t = 52) and a validation set ( S v = 40). First, a deep learning–based model was developed to segment myeloblasts. A total of 214 texture and shape descriptors were then extracted from the segmented myeloblasts on aspirate slide images. A risk score on the basis of texture features of myeloblast chromatin patterns was generated by using the least absolute shrinkage and selection operator with a Cox regression model. RESULTS The risk score was associated with RFS in S t (hazard ratio = 2.38; 95% CI, 1.4 to 3.95; P = .0008) and S v (hazard ratio = 1.57; 95% CI, 1.01 to 2.45; P = .044). We also demonstrate that this resulting signature was predictive of AML relapse with an area under the receiver operating characteristic curve of 0.71 within S v. All the relevant code is available at GitHub. CONCLUSION The texture features extracted from chromatin patterns of myeloblasts can predict post-HCT relapse and prognosticate RFS of patients with AML/MDS.
Grief Aesthetics
Authors: Ife Fahima 
Published: May 2022

Abstract
An excerpt from an experimental series, “Grief Aesthetics” is a lyrical essay on intimacy and writing. The essay participates in the double black study of eroding and composing a new sentence, a new sentence sounded in refusal (of the social pact of writing, of grammar), a new sentence forged in friendship (of thinking with, of writing with). On one level, “Grief Aesthetics” is concerned with inherited grief, the remains of stolen life, the residual desire for romance in contemporary black art. The essay considers the loss of romantic love in conversation with Terence Nance's feature and short films An Oversimplification of Her Beauty and Swimming in Your Skin Again, in conversation with a single recurring line from a poem in Taylor Johnson's Inheritance. At another level, “Grief Aesthetics” is a sort of ars poetica on the process of writing and at this level is preoccupied with the poetic line, the sentence (thinking about sentences), the space in writing, the mundane moments in between the emergence of a line or series of lines and declension as it occurs in the gaps. At every turn “Grief Aesthetics” practices creating in “commonsense” (a shared feeling, a shared aesthetics) among contemporary artists whose works evoke a sense of grief, (dis)placement, loss, loneliness, homelessness, desire, tenderness, friendship, bliss.
The role of Lrp6-mediated Wnt/β-catenin signaling in the development and intervention of spinal neural tube defects in mice
Authors: Zhao Tianyu , McMahon Moira , Reynolds Kurt , Saha Subbroto Kumar , Stokes Arjun , Zhou Chengji J. 
Published: May 2022

ABSTRACT
Neural tube defects (NTDs) are among the common and severe birth defects with poorly understood etiology. Mutations in the Wnt co-receptor LRP6 are associated with NTDs in humans. Either gain-of-function (GOF) or loss-of-function (LOF) mutations of Lrp6 can cause NTDs in mice. NTDs in Lrp6-GOF mutants may be attributed to altered β-catenin-independent noncanonical Wnt signaling. However, the mechanisms underlying NTDs in Lrp6-LOF mutants and the role of Lrp6-mediated canonical Wnt/β-catenin signaling in neural tube closure remain unresolved. We previously demonstrated that β-catenin signaling is required for posterior neuropore (PNP) closure. In the current study, conditional ablation of Lrp6 in dorsal PNP caused spinal NTDs with diminished activities of Wnt/β-catenin signaling and its downstream target gene Pax3, which is required for PNP closure. β-catenin-GOF rescued NTDs in Lrp6-LOF mutants. Moreover, maternal supplementation of a Wnt/β-catenin signaling agonist reduced the frequency and severity of spinal NTDs in Lrp6-LOF mutants by restoring Pax3 expression. Together, these results demonstrate the essential role of Lrp6-mediated Wnt/β-catenin signaling in PNP closure, which could also provide a therapeutic target for NTD intervention through manipulation of canonical Wnt/β-catenin signaling activities.
Inter-Spin Interactions of 1D Chains of Different-Sized Nitroxide Radicals Incorporated in the Organic 1D Nanochannels of Tris(o-phenylenedioxy)cyclotriphosphazene
Authors: Kobayashi Hirokazu , Takeuchi Kanae , Morinaga Yuka , Honda Hidehiko , Yamamoto Masato , Odanaka Yuki , Inagaki Masahiro 
Published: May 2022

Abstract
Variable-temperature electron spin resonance (ESR) was measured for one-dimensional (1D) molecular chains formed using different-sized organic radicals incorporated into the 1D nanochannels of tris(o-phenylenedioxy)cyclotriphosphazene (TPP). The ESR spectra for the molecular chains of 4-oxo-2,2,6,6-tetramethyl-1-piperidinyl-1-oxyl (TEMPONE) incorporated in the TPP nanochannels ([TPP-TEMPONE]) exhibited anisotropic three-dimensional (3D) spin diffusion at temperatures close to room temperature. In contrast, 1D spin diffusion was observed even at low temperatures indicating a longer rotational diffusion correlation time or the termination of molecular motion of the guest radicals dispersed in the TPP nanochannels. The temperature range for 1D spin diffusion in [TPP-TEMPONE] was higher and wider than that of TPP inclusion compounds incorporating smaller nitroxide radicals, such as di-t-butyl nitroxide (DTBN), or 2,2,6,6-tetramethyl-1-piperidinyl-1-oxyl (TEMPO) radicals, as previously reported. Thus, inter-spin interactions of the organic-radical 1D molecular chains formed in size-adjustable nanochannels, such as TPP, are influenced by the molecular size and dynamics of guest radicals, and temperature.
Meniscus Root Tears: A Clinical Review
Authors: Randazzo Elena , Duerr Robert , Baria Michael R. 
Published: May 2022

Abstract
Meniscus root tears are important to recognize early given their potentially devastating consequences on joint health. This injury results in the lost ability of the meniscus to transfer axial loads into hoop stress; therefore, it is functionally equivalent to a complete meniscectomy. This causes rapid progression of osteoarthritis and increased need to total knee arthroplasty in a previously healthy joint. Despite these consequences, root tears have only been discussed in the orthopedic literature in the last 10 to 15 years and have not been routinely integrated into nonoperative sports medicine education. It is important for all nonoperative sports medicine providers to properly diagnose and triage this injury early in its course to maximize joint preservation efforts. The goal of this manuscript is to review the anatomy, presentation, natural history, imaging, and treatment options for meniscal root tears.
Patient preferences regarding communication of minor surgery results: who and how?
Authors: Senaratne Radha , Casey Máire-Caitlín , Balakrishnan Theogren , Hussey Alan , Jones Deirdre , McInerney Niall , Regan Padraic J , Kelly Jack L 
Published: May 2022

Background/Aims The excision of skin lesions is one of the most common procedures in plastic surgery. Following the disruptions associated with the COVID-19 pandemic, this study investigated patient preferences communicating the results of skin lesion excision surgery. Methods A retrospective analysis was conducted with patients who had undergone elective minor surgical procedures over a 30-day period. A questionnaire was distributed to 103 patients to assess their preferences regarding delivery of the outcomes of their procedure in terms of which healthcare professional contacted them and whether this occurred in person or remotely. The outcomes of this were used to adapt the service and a repeat analysis was conducted 3 months later. Results If the lesion was benign, 45.0% of participants preferred to be contacted via email, letter or text, while 38.8% preferred either a telephone call or in-person appointment with their GP, and only 16.3% preferred direct contact from their hospital doctor. If the lesion was malignant, 55.0% preferred to be contacted by their GP, 30.0% by their hospital doctor and 15.0% via letter, email or text. The repeat analysis saw the proportion of patients who received their results via remote consultation with a hospital clinician increase from 12.8% to 16.1%, while the proportion of patients who received their results at an in-person outpatients appointment decreased from 14.5% to 9.3%. Conclusions This study shows that a substantial proportion of patients would prefer to receive the results of skin lesion surgery from their GP over the telephone, suggesting that this would be a better means of delivering results than an in-person hospital appointment. This method could also reduce the number of patients returning to the clinic in person, helping to streamline follow up and improve clinic efficiency.
Comparison of periprosthetic femoral fracture torque and strain pattern of three types of femoral components in experimental model
Authors: Takegami Yasuhiko , Seki Taisuke , Osawa Yusuke , Imagama Shiro 
Published: May 2022

Aims Periprosthetic hip fractures (PPFs) after total hip arthroplasty are difficult to treat. Therefore, it is important to identify modifiable risk factors such as stem selection to reduce the occurrence of PPFs. This study aimed to clarify differences in fracture torque, surface strain, and fracture type analysis between three different types of cemented stems. Methods We conducted biomechanical testing of bone analogues using six cemented stems of three different types: collarless polished tapered (CPT) stem, Versys Advocate (Versys) stem, and Charnley-Marcel-Kerboull (CMK) stem. Experienced surgeons implanted each of these types of stems into six bone analogues, and the analogues were compressed and internally rotated until failure. Torque to fracture and fracture type were recorded. We also measured surface strain distribution using triaxial rosettes. Results There was a significant difference in fracture torque between the three stem types (p = 0.036). Particularly, the median fracture torque for the CPT stem was significantly lower than that for the CMK stem (CPT vs CMK: 164.5 Nm vs 200.5 Nm; p = 0.046). The strain values for the CPT stem were higher than those for the other two stems at the most proximal site. The fracture pattern of the CPT and Versys stems was Vancouver type B, whereas that of the CMK stem was type C. Conclusion Our study suggested that the cobalt-chromium alloy material, polished surface finish, acute-square proximal form, and the absence of a collar may be associated with lower fracture torque, which may be related to PPF. Cite this article: Bone Joint Res 2022;11(5):270–277.
Association of type 2 diabetes with periodontitis and tooth loss in patients undergoing hemodialysis
Authors: Mikami Risako , Mizutani Koji , Matsuyama Yusuke , Gohda Tomohito , Gotoh Hiromichi , Aoyama Norio , Matsuura Takanori , Kido Daisuke , Takeda Kohei , Saito Natsumi , Fujiwara Takeo , Izumi Yuichi , Iwata Takanori 
Published: May 2022

Background
Limited evidence are available regarding the influence of diabetes on periodontitis in hemodialysis patients, although the association between diabetes and periodontal disease is well-known.

Objective
This study aimed to investigate the influence of type 2 diabetes mellitus (T2D) and its control level on periodontal disease and the number of missing teeth in patients undergoing hemodialysis.

Subjects and methods
A single-center cross-sectional study was conducted on 246 Japanese patients with end-stage renal disease undergoing hemodialysis. Comprehensive medical and dental examinations were performed. The association between severity of periodontitis and T2D was examined by multiple ordered logistic regression analysis. A multiple linear regression model was fitted to assess the association of periodontal probing depth (PPD) ≥4 mm and the number of missing teeth with T2D (n = 125). A subgroup analysis involving only the patients with T2D was performed to investigate the factors associated with missing teeth among them.

Results
After adjusting for confounders, the classification of periodontitis severity was significantly advanced in patients with T2D (odds ratio: 1.64, 95% confidence interval [CI]: 1.02–2.65, p = 0.04). The proportion of PPD≥4 mm sites and the number of missing teeth was significantly associated with T2D (coefficient: 4.1 and 5.7, 95% CI: 0.2–8.0 and 3.4–8.0, p = 0.04 and <0.001, respectively). Subgroup analysis of T2D patients revealed that glycoalbumin levels (coefficient: 0.4, 95% CI: 0.03–0.80, p = 0.03), but not hemoglobin A1c levels (coefficient: 0.8, 95% CI: -1.0–2.7, p = 0.37), were significantly associated with the number of missing teeth.

Conclusion
T2D was significantly associated with periodontitis and the number of missing teeth in hemodialysis patients. Moreover, it is first documented that poor glycemic control, as determined by glycoalbumin levels, was significantly associated with the number of missing teeth in hemodialysis patients with T2D.
Efficacy and safety of combined oral sucrose and nonnutritive sucking in pain management for infants: A systematic review and meta-analysis
Authors: Li Qiaohong , Tan Xuerong , Li Xueqing , Tang Wenxiu , Mei Lin , Cheng Gang , Zou Yongrong 
Published: May 2022

Background
Pain management is currently important in neonatal intensive care unit (NICU). The superiority in pain relief of the combined oral sucrose (OS) and nonnutritive sucking (NNS) to other single intervention has not been well established. The administration of sucrose has been considered to potentially induce adverse events, which has been controversial. This study aims to investigate the combined effects and safety in comparison with other single intervention methods, including NNS, OS alone, breast milk and oral glucose.

Methods
We searched databases including Medline (via Pubmed), Embase (via Ovid), web of science, and Cochrane Library for randomized controlled trials from Jan 1, 2000 to Mar 31, 2021. The data were analyzed in the meta-analysis using Review manager Version 5.3. Pain score was the primary outcome in this meta-analysis. The adverse events were assessed qualitatively.

Results
A total of 16 studies were eligible in the meta-analysis. The results demonstrated a significant reduction in pain score in the NNS+OS group compared with NNS alone (SMD = -1.69, 95%CI, -1.69,-0.65) or sucrose alone (SMD = -1.39, 95% CI, -2.21,-0.57) during the painful procedures. When compared NNS+OS with breast milk, no significant difference was detected (SMD = -0.19, 95% CI: -0.5, 0.11).

Conclusion
The combined effects of NNS and OS might be superior to other single intervention method. However, the effects might be mild for moderate-to-severe pain.
Physical activity and social support are associated with quality of life in middle-aged women
Authors: Nguyen Thao Thi Phuong , Phan Hai Thanh , Vu Thuc Minh Thi , Tran Phuc Quang , Do Hieu Trung , Vu Linh Gia , Doan Linh Phuong , Do Huyen Phuc , Latkin Carl A. , Ho Cyrus S. H. , Ho Roger C. M. 
Published: May 2022

Purposes
This cross-sectional study assessed the quality of life and related factors of Vietnamese women during perimenopause in terms of vasomotor, psychosocial, physical, and sexual aspects.

Materials and methods
A cross-sectional study on 400 middle-aged women was conducted in Hung Yen, a delta province in Vietnam. Data about socioeconomic characteristics, daily activity patterns, quality of life in terms of vasomotor, psychosocial, physical, and sexual aspects, and level of social support were collected. Tobit multivariate regression model was used to identify factors related to the quality of life among participants.

Results
The symptoms of perimenopause appeared to worsen with the increase of age and the existence of such health issues as migraine and diabetes. Meanwhile, exercises, recreational activities, and social support appeared to alleviate the negative impact of perimenopausal symptoms on women.

Conclusions
It is important to address the care needs of women during perimenopausal age, especially their sexual well-being, and development of specific healthcare services and programs focusing on sport, entertainment, and support for women in perimenopause should be facilitated.