bluuebunny/crossref_metadata_2025_split huggingface dataset:
ZFPM2-AS1: An Oncogenic Long Non-coding RNA in Multiple Cancer
Types
Authors: Tan Fangshun 
Published: May 2022

Abstract:
Long non-coding RNA (lncRNA) is a novel kind of RNA transcript with lengths greater
than 200 nucleotides. Functionally, lncRNAs lack the potential to encode peptides or proteins.
Previous studies unveiled that lncRNA participated in numerous physiological and pathological
processes, including cancer, aging, and immune responses. Newly discovered long noncoding
RNA zinc finger protein, Friend of GATA (FOG) family member 2‑antisense 1 (ZFPM2-AS1),
located on the 8q23 chromosome, acts as a tumor stimulator in various cancer types, including
Breast Cancer (BC), Colorectal Cancer (CRC), Esophageal Squamous Cell Carcinoma (ESCC),
Gastric Cancer (GC), glioma, hepatocellular carcinoma (HCC), Lung Adenocarcinoma (LUAD),
melanoma, non-small cell lung cancer (NSCLC), Retinoblastoma (RB), Small Cell Lung Cancer
(SCLC) and thyroid cancer. Accumulative evidence also elucidated that ZFPM2-AS1 dysregulation
was related to tumor proliferative, migratory, invasive, anti-apoptotic, and pro-epithelial-tomesenchymal
Transition (EMT) effects, larger tumor volume, higher tumor weight, advanced tumor
stage, high rates of lymphatic metastasis, distant metastasis, poor prognosis, histological differentiation,
higher TNM (tumor, node, metastases) stage, depth of tumor invasion, reduced overall and disease-
free survival, vein invasion, and shorter 5-year overall survival. Mechanistically, ZFPM2-AS1
acted as a ceRNA to play its oncogenic role. Thus, this study summarized the specific mechanisms
of the lncRNA ZFPM2-AS1 in the aforementioned cancer types to reveal its novel application in
cancer diagnosis, treatment, and prognosis.
Anti-TLR4 IgG2 Prevents Acetaminophen-induced Acute Liver
Injury through the Toll-like Receptor 4/MAPKs Signaling Pathway
in Mice
Authors: Yao Chuanxia , Wang Yiwen , Gong DanDan , Feng Tian , Zhang Yaqiong , Wang Chunhui , Wang Maorong , Zhu Jin 
Published: May 2022

Background and Objective:
Acetaminophen (APAP) is a widely used antipyretic and
analgesic. If taken in excess, it can cause severe drug-induced acute liver injury. The purpose of
this study was to investigate the effects of anti-TLR4 IgG2 on APAP-induced liver injury and its
underlying mechanisms.

Methods:
We injected APAP into the abdominal cavity of mice to establish a liver injury model.
Mice were divided into the control group, APAP group, and APAP + anti-TLR4 IgG2 group. In order
to verify the implication of the toll-like receptor4 and mitogen-activated protein kinases activation
(TLR4/MAPKs) signaling pathway, mice were intraperitoneally injected with a TLR4 / MAPKs
inhibitor anti-TLR4 IgG2. We evaluated the effects of TLR4 IgG2 on the antioxidant, anti-apoptotic,
anti-inflammatory, and liver histopathology of APAP mice. In addition, the expression of the TLR4 /
MAPKs signaling pathway was detected by Western blot.

Results:
Our study showed that APAP mouse models were successfully established; however,
pretreatment with anti-TLR4 IgG2 alleviated APAP-induced hepatic injury, as evidenced by the 24-h
survival rate. Meanwhile, anti-TLR4 IgG2 prevented the elevation of serum biochemical parameters
and lipid profile. Furthermore, compared with the APAP group, hepatic antioxidants, including 3-
Nitrotyrosine, high mobility group protein B1, superoxide dismutase, catalase, and glutathione, were
increased in APAP + anti-TLR4 IgG2 group. In contrast, a significant decrease was observed in the
levels of the malondialdehyde, which is a lipid peroxidation product. Moreover, the western blotting
analysis showed that anti-TLR4 IgG2 treatment inhibited the activation of the apoptotic pathway by
increasing Bcl-2 and decreasing Bax, P53, and cleaving caspase-3 / caspase-3 protein expression.
These results were further validated by TUNEL staining and immunohistochemical.
Histopathological observation also revealed that pretreat-ment with anti-TLR4 IgG2 could
significantly reverse hepatocyte inflammatory infiltration, congestion, and necrosis in liver tissues by
APAP. Importantly, anti-TLR4 IgG2 effectively alleviated APAP-induced liver injury by inhibiting tolllike
receptor4 and mitogen-activated protein kinases activation signaling pathways (TLR4/MAPKs).

Conclusion:
The results clearly suggest that the underlying molecular mechanisms in the
hepatoprotection of anti-TLR4 IgG2 in APAP-induced hepatotoxicity may be due to its antioxidation,
anti-apoptosis, and anti-inflammation effects through inhibition of the TLR4/MAPKs
signaling axis.
Spray Drying as an Effective Method in the Development of Solid Self-
Emulsifying Drug Delivery Systems
Authors: Chawla Pooja A. , Kumar Mohit , Faruk Abdul , Chawla Viney 
Published: May 2022

Abstract:
Most of the new drug candidates and present ones are lipophilic, which leads to low bioavailability.
Self-emulsifying drug delivery systems (SEDDS) have emerged as promising formulation
system for poorly water-soluble drug candidates. Over the last two decades, various such drug compounds
were used by researchers for the development of SEDDS. At present, many SEDDS formulations
are also available in the market. Though SEDDS offer many advantages but drawbacks like low
drug loading, few dosage form choices, difficulty in handling and storage led to the solidification of
this system by various methods. Solidification by spray drying technique offers a lot of advantages like
scalability and stability. This particular method is the focus of this review. Adsorbent carriers have the
most significant role in the fate of this formulation and its compatibility with the drug candidate. This
review addresses the advantages, method of development, spray drying specifications, and characterization
of S-SEDDS in detail. Furthermore, the prospect of turning spray-dried SEDDS into tablets by
punching which offers potential advantages of increased bioavailability and stability has also been
discussed.
Development and Evaluation of Biotin Functionalized Fullerenes for the
Delivery of Irinotecan to Colon Tumors
Authors: Dhiman Shikha , Kaur Amardeep , Gupta Girdhari L. , Sharma Manu 
Published: May 2022

Background:
Background: Irinotecan is a promising antitumor agent approved by FDA for intravenous
use in colon cancer treatment either alone or in combination. It is a topoisomerase inhibitor and by
blocking the topoisomerase-I enzyme, it causes DNA damage and results in cell death. However, it
lacks selectivity and specificity for tumor cells, resulting in systemic toxicity. Thus, it is essential to
reduce its side effects and improve therapeutic efficacy.

Objective:
The study aimed to improve the therapeutic efficacy and minimize the toxic effects of irinotecan
by developing a fullerene functionalized biotin drug delivery system and adsorbing irinotecan
on the surface of the functionalized fullerene-biotin complex.

Methods:
Fullerene (C60) has been observed as a potential drug delivery agent and the aminefunctionalized
C60-NH2 was synthesized by functionalizing ethylenediamine on the surface of C60. The
PEI functionalized C60 was further synthesized by polymerization of aziridine on the surface of C60-
NH2. Biotin was attached by an amide linkage to C60-PEI and the anti-colon cancer drug irinotecan (IRI)
was encapsulated (C60-PEI-Biotin/IRI). The C60-PEI-Biotin/IRI was characterized and evaluated for in
vivo anti-colon cancer activity in rats and the results were compared with the parent drug irinotecan.

Results:
The results showed that C60-PEI-Biotin/IRI conjugate had a controlled release profile according
to in vitro HPLC studies. Moreover in vivo anti-tumor studies suggested that the conjugate proved to be
less toxic to vital organs and had high efficacy towards tumor cells. Statistical studies confirmed less
tumor index and tumor burden in the case of conjugate when compared to irinotecan.

Conclusion:
It is hypothesized that the conjugate (C60-PEI-Biotin/IRI) could cross the cell membrane
easily through overexpressed biotin receptors on the cell surface of colon cancer cells and showed better
efficacy and less toxicity in comparison to IRI in the colon cancer rat model.
A Concise Review on the Role of Natural and Synthetically Derived
Peptides in Colorectal Cancer
Authors: Das Alakesh , Deka Dikshita , Banerjee Antara , Radhakrishnan Arun Kumar , Zhang Hong , Sun Xiao-Feng , Pathak Surajit 
Published: May 2022

Abstract:
Colorectal cancer being the second leading cause of cancer-associated deaths has become a significant health concern around the globe. Though there are various cancer treatment approach-es, many of them show adverse effects and some compromise the health of cancer patients. Hence, significant efforts are being made for the evolution of a novel biological therapeutic approach with better efficacy and minimal side effects. Current research suggests that the application of peptides in colorectal cancer therapeutics holds the possibility of the emergence of an anticancer reagent. The primary beneficial factors of peptides are their comparatively rapid and easy process of synthesis and the enormous potential for chemical alterations that can be evaluated for designing novel pep-tides and enhancing the delivery capacity of peptides. Peptides might be utilized as agents with cy-totoxic activities or as a carrier of a specific drug or as cytotoxic agents that can efficiently target the tumor cells. Further, peptides can also be used as a tool for diagnostic purposes. The recent analysis aims at developing peptides that have the potential to efficiently target the tumor moieties without harming the nearby normal cells. Additionally, decreasing the adverse effects, and unfold-ing the other therapeutic properties of potential peptides, are also the subject matter of in-depth analysis. This review provides a concise summary of the function of both natural and synthetically derived peptides in colorectal cancer therapeutics that are recently being evaluated and their potent applications in the clinical field.
COVID-19 in G6PD-deficient Patients, Oxidative Stress, and Neuropathology
Authors: Hernández-Ochoa Beatriz , Ortega-Cuellar Daniel , González-Valdez Abigail , Cárdenas-Rodríguez Noemí , Mendoza-Torreblanca Julieta Griselda , Contreras-García Itzel Jatziri , Pichardo-Macías Luz Adriana , Bandala Cindy , Gómez-Manzo Saúl 
Published: May 2022

Abstract:
Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme that regulates energy metabolism
mainly through the pentose phosphate pathway (PPP). It is well known that this enzyme participates
in the antioxidant/oxidant balance via the synthesis of energy-rich molecules: nicotinamide
adenine dinucleotide phosphate reduced (NADPH), the reduced form of flavin adenine dinucleotide
(FADH) and glutathione (GSH), controlling reactive oxygen species generation. Coronavirus disease
19 (COVID-19), induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),
is a public health problem that has caused approximately 4.5 million deaths since December 2019.
Concerning the role of G6PD in COVID-19 development, it is known from the existing literature
that G6PD-deficient patients infected with SARS-CoV-2 are more susceptible to thrombosis and
hemolysis, suggesting that G6PD deficiency facilitates infection by SARS-CoV-2. Concerning
G6PD and neuropathology, it has been observed that deficiency of this enzyme is also present with
an increase in oxidative markers. Concerning the role of G6PD and the neurological manifestations
of COVID-19, it has been reported that the enzymatic deficiency in patients infected with SARSCoV-
2 exacerbates the disease, and, in some clinical reports, an increase in hemolysis and thrombosis
was observed when patients were treated with hydroxychloroquine (OH-CQ), a drug with oxidative
properties. In the present work, we summarize the evidence of the role of G6PD in COVID-
19 and its possible role in the generation of oxidative stress and glucose metabolism deficits, and
inflammation present in this respiratory disease and its progression including neurological manifestations.
Molecular Docking Simulation-based Pharmacophore Modeling to Design
Translation Inhibitors Targeting c-di-GMP Riboswitch of Vibrio cholera
Authors: Mujwar Somdutt , Pardasani Kamalraj 
Published: May 2022

Background:
Vibrio cholera is a facultative pathogenic bacterium that causes cholera pandemics, primarily in nations with hot and humid climates and large bodies of water containing a large quantity of organic debris. Consumption of V. cholera contaminated water or food causes acute diarrheal illness, followed by severe dehydration and mortality. Cholera is a highly infectious illness, with over 4 million cases recorded globally each year, and over a hundred thousand deaths.

Objective:
The only known therapy for cholera infection is oral rehydration solution along with antibiotics. Excessive antibiotic use causes pathogens to acquire antimicrobial drug resistance, resulting in a loss of efficacy. Furthermore, antibiotics are accompanied with a plethora of unfavorable side effects, restricting their usage.

Methods:
A Riboswitch is a non-homologous proteinaceous therapeutic target that plays a regulatory role in the crucial process of bacterial translation. As a result, the bacterial riboswitch was investigated as a surrogate target for developing a therapeutic medication against V. cholera.

Results:
In-silico screening with 24407 ligands was performed against the bacterial riboswitch to identify potential lead candidates, followed by pharmacophore modeling and bioisosteric lead modifications to design potential leads having an antagonistic impact on the pathogenic bacterial riboswitch

Conclusion:
The riboswitch-based innovative therapy was anticipated to be devoid of the issues connected with the development of antimicrobial drug resistance as well as the unwanted side effects associated with antibiotic usage.
Renal Artery Resistive Index and Estimated Glomerular Filtration Rate in Patients with Non-alcoholic Fatty Liver Disease
Authors: Aksu Yusuf , Uslu Ali Ugur , Tarhan Gulten , Tiryaki Şaban 
Published: May 2022

Objectives:
Non-alcoholic fatty liver disease (NAFLD) may affect many organs and systems,
especially the kidney as well as the liver. While NAFLD’s renal impacts can be evaluated via the estimated
glomerular filtration rate (eGFR), its effects on renal hemodynamic changes can be monitored
with the renal resistive index (RRI). Our aim in this study is to evaluate RRI and eGFR in NAFLD
patients.

Materials and methods:
In this study, a total of 69 patients with NAFLD and 50 age- and gendermatched
healthy controls were included. The basic clinical and laboratory parameters of patient and
control groups were assessed and recorded.

Results:
In the patient group, the RRI was observed as 0.64±0.06 and eGFR value as 86±52
(mL/min/1.73m2), while in the control group, the RRI was 0.61±0.05 and eGFR was 95.40±20.21
(mL/min/1.73m2). Compared to the control group, the RRI and eGFR values were significantly different
in the patient group (PRRI=0.003 and PeGFR=0.025). There was a negative correlation observed
between the RRI and eGFR (r=-0.347, P=0.003). Receiver-operating characteristic curve analysis suggested
that the optimum RRI cut-off value for patients with NAFLD is 0.62, with 65% sensitivity and
60% specificity (p=0.002). There was an independent relationship found between the RRI and eGFR
according to the linear regression analysis (β=-0.301, P=0.015).

Conclusion:
This study shows that RRI may be an indicator of renal functions, such as eGFR in patients
with NAFLD.
Microwave-Assisted: An Efficient Aqueous Suzuki-Miyaura Cross-
Coupling Reaction of the Substituted 1H-1,2,3-Triazoles
Authors: Al-Harrasi Ahmed , Avula Satya Kumar , Rehman Najeeb Ur , Csuk Rene , Das Biswanath 
Published: May 2022

Background:
A variety of natural products reveal the presence of the 1H-1,2,3-triazole
moiety in their chemical structures. In general, these molecules also play a significant role in the
agrochemical, medicinal and pharmaceutical industries. Microwave-assisted reactions have attracted
great interest for researchers to synthesize 1H-1,2,3-triazole compounds in shorter times with
increased yields.

Objective:
The objective of this study is to optimize the purity and yield of the product, shorter the
reaction time, and make the reaction more eco-friendly with the help of microwave-assisted organic
synthesis.

Methods:
The present work elucidates a very simple but efficient and rapid, highly productive
synthesis of various substituted 1H-1,2,3-triazole series, using the Suzuki-Miyaura cross-coupling
reaction, employing microwave irradiation in water with tetrabutylammonium bromide (TBAB).
Utilizing (S)- (-) ethyl lactate as the starting material, the synthesis of the substituted 1H-1,2,3-
triazole aryl bromide (1) was achieved.

Results:
This compound (1) was subjected to the Suzuki-Miyaura cross-coupling reaction under
microwave irradiation, using a variety of aryl boronic acids in an aqueous medium, to attain high
yields of the target products, namely 3a-w. Overall, this is an environmentally benign, very efficient
technique under microwave irradiations as a green and eco-friendly source. Only those methodologies
that involve microwave-assisted reactions during synthesis in a related manner have
been reviewed.

Conclusion:
Microwave-assisted Suzuki-Miyaura cross-coupling reactions in the water of substituted
1H-1,2,3-triazole series can be employed to quickly explore and increase molecular diversity
in synthetic chemistry. In this respect, microwave-mediated methods help researchers to make
helpful studies.
Investigating the Effect of Hydroxyl Functionalized MWCNT on the
Mechanical Properties of PMMA-Based Polymer Nanocomposites
Authors: Patel Vijay , Joshi Unnati , Joshi Anand 
Published: May 2022

Aims:
The aim of this study was to evaluate the mechanical properties of polymer nanocomposites (PNCs) reinforced with multiwalled carbon nanotubes (MWCNT).

Methods:
Mixing MWCNT into the polymer at very small propositions can enrich the mechanical properties of the polymer nanocomposites. Testing Specimen fabricated with 0.1wt%, 0.5wt%, 1wt% mixing ratios using extrusion and injection molding process. Computational analysis was performed through the square RVE model and analyzed with finite element analysis (FEA) using DIGIMAT simulation tool. The specimen was evaluated by ASTM D3039 for tensile strength and ASTM D7264 for flexural.

Results:
The simulated results were compared with experimental results. Scanning electron microscopy(SEM) was performed to evaluate the dispersion state of nanotubes in the matrix.

Conclusion:
The impactful improvement in mechanical properties is shown in the results after addition of functionalized MWCNTs (f-MWCNT) compared to pure polymer and non- functionalized MWCNT composites.
Hologram QSAR and Topomer CoMFA Study on Naphthyridone
Derivatives as ATAD2 Bromodomain Inhibitors
Authors: lv Zhenxia , Ren Jing , Zhang Shaobo , Wang Bokai , Peng Yuxin , Yao Wu , Yuan Jintao 
Published: May 2022

Background:
ATAD2 is closely related to the occurrence and proliferation of many
tumors. Thus, exploring ATAD2 inhibitors is greatly significant for the prevention and treatment
of tumors. In this study, the quantitative structure–activity relationship (QSAR) analyses of 57
naphthyridone derivatives were conducted using hologram quantitative structure–activity
relationship (HQSAR) and topomer comparative molecular field analysis (topomer CoMFA).

Method:
The 57 naphthyridone derivatives were divided into the training (44 derivatives) and
testing (13 derivatives) sets. HQSAR and topomer CoMFA models were obtained by applying the
SYBYL-X software and validated using various validation parameters. Contribution maps from the
best HQSAR model and the contour maps from the best topomer CoMFA model were analyzed.

Results:
The most effective HQSAR model exhibited significant cross-validated (q2 = 0.872) and non cross-validated (r2 = 0.972) correlation coefficients, and the most effective topomer CoMFA model had q2 = 0.861 and r2 = 0.962. Several external validation parameters, such as , , , , and , were used to calculate the correlation coefficients of the test set samples and validate both models. The result exhibited a powerful predictive capability. Graphical results from HQSAR and topomer CoMFA were validated by the binding mode in the crystal structure.

Conclusion:
The models may be beneficial to enhance the understanding of the structure–activity
relationships for this class of compounds and also provide useful clues for the design of potential
ATAD2 bromodomain inhibitors.
Codevelopment of Implementation Interventions to Support Parent-Led Care for Pain in Infants: Protocol for a Qualitative Descriptive Study
Authors: Benoit Britney , Cassidy Christine , van Wijlen Jacqueline , Correll Quinn , Campbell-Yeo Marsha , Hendra Sionnach , Martin-Misener Ruth , MacDougall Jennifer , Cameron Ashley 
Published: May 2022

Background
Untreated pain in infants is associated with adverse health outcomes. Despite strong evidence for accessible, effective, and low-cost parent-led pain-relieving interventions such as breastfeeding or chestfeeding and skin-to-skin contact, these interventions are not routinely used.

Objective
The objective of this study is to support the implementation of parent-led pain interventions by identifying barriers to and facilitators of parent-led, evidence-informed pain care in infants during acute procedures. In addition, this study aims to develop theory-informed, contextually relevant implementation interventions for supporting the use of parent-led pain care for infants in hospital and community contexts.

Methods
This study will consist of 2 phases that follow a systematic, theoretically informed approach guided by the Theoretical Domains Framework and Behavior Change Wheel. In phase 1, we will use a qualitative descriptive design to explore barriers and facilitators to using parent-led pain care in infants from the perspectives of hospital and community-based clinicians, clinical leaders, and families. In phase 2, we will use the Behavior Change Wheel to design tailored implementation interventions that have evidence for effectively addressing identified barriers in collaboration with an advisory committee of administrative, clinical, and family leaders.

Results
Ethics approval for this study was obtained in December 2020. As of May 2022, a total of 15 participants have been enrolled in phase 1. The results from all phases will be reported in 2023.

Conclusions
Following the completion of this study, we will have co-designed theoretically informed implementation interventions that can be pilot-tested and experimentally applied. The findings will be used to implement parent-led interventions that improve patient safety and health outcomes for diverse families.

International Registered Report Identifier (IRRID)
DERR1-10.2196/33770