bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of In Vitro Skin Permeation of Enzymatically Synthesized Phloridzin Acetates from Emulsions and Liposomes Dispersed in Gel
Authors: Milivojević Ana , Ćorović Marija , Simović Milica , Banjanac Katarina , Pjanović Rada , Bezbradica Dejan 
Published: August 2022

AbstractThis work aims to evaluate the effect of cosmetic formulations on the skin permeation of phloridzin acetates, therefore in vitro transdermal delivery of these bioactive compounds across Strat‐M membrane is investigated. The formulations tested are gel emulsion, oil in water emulsion (O/W emulsion), gel in oil emulsion, liquid crystal emulsion, and liposomes dispersed in gel. The release of esters from the O/W emulsion is diffusion‐controlled (n = 0.5), while release kinetics from gel‐based formulations depends on coupled diffusion and polymer relaxations effect (0.5 < n < 1). The highest values of effective diffusion coefficient are obtained after release from O/W emulsion (24.64 × 10−10 m2 min−1 for phloridzin acetate and 6.55 × 10−10 m2 min−1 for phloridzin diacetate). The lag phase lasting from 1 h (gel emulsion) to 5 h (liquid crystal emulsion) indicates that esters permeation is significantly affected by the formulation used. Formulations in which the water is continuous phase are the most appropriate carrier system for transdermal delivery of phloridzin esters. Additionally, liposomes are successfully applied as carrier systems for the controlled release of esters, although it should be noted that liposomes without cholesterol show remarkable stability (ζ = −43 mV) and homogeneity (polydispersity index = 0.198).Practical application: Phloridzin is a flavonoid glycoside that possesses many beneficial activities such as antioxidant, UV‐protecting, and anti‐inflammatory activity, so the use of this flavonoid and its derivatives in numerous cosmetic and pharmaceutical formulations seems very attractive. Results obtained in this study give a comprehensive insight into the release mechanism of phloridzin acetates from emulsions and liposomes, enable the selection of optimum cosmetic formulations depending on the application, and provide significant scientific contribution in the field of topical administration of bioactive flavonoid derivatives.
Seismic evaluation of friction spring‐based self‐centering braced frames based on life‐cycle cost
Authors: Zhang Ruibin , Wang Wei , Alam M. Shahria 
Published: August 2022

AbstractIn recent decades, a variety of self‐centering braces (SCBs) have been developed to address the limitations of conventional frames by decreasing residual drift due to earthquakes. However, the initial construction cost of self‐centering (SC) structures is expected to be higher and the study about its cost‐effectiveness over the life‐cycle span is limited. This paper presents a seismic life‐cycle cost evaluation of emerging friction spring‐based self‐centering braced frames (SCB‐Fs) compared with traditional buckling restrained braced frames (BRB‐Fs) when an existing structure is upgraded. Particular focus is on the effect of residual deformation, initial construction cost, and high fatigue performance of the SCB. Following the performance‐based design of the SCB‐F and BRB‐F, system‐level analyses are conducted. Numerical results of case‐study buildings indicate that the total expected annual loss (EAL) of the BRB‐F increases by approximately three times when the effect of residual deformation is considered, while its effect on the total EAL of the SCB‐F is negligible. Besides, the superior performance of the SCB‐F compared to BRB‐F is highlighted by a substantial reduction in EAL induced by earthquakes. In addition, the acceleration‐related seismic losses of SCB‐F constitute approximately 44% of the total EAL. Its contribution is significantly larger in the case of the SCB‐F compared to the BRB‐F. From the perspective of economic benefit, increasing the structural life‐cycle span is beneficial to the SCB‐F compared to the BRB‐F. The high fatigue performance of the SCB is favorable to increase the economic benefit of the SCB‐F, especially when the reduction of repair time is considered. The economic benefit of the SCB‐F compared to the BRB‐F is highly related to the initial construction cost. Taking the 100 years as an expected life‐cycle span, the high initial construction cost of the SCB‐F would not be paid off when the unit cost of the SCB is about 2.1 times that of the BRB.
A cost‐aware approach for cloud federation formation
Authors: Dinachali Bijan Pourghorbani , Jabbehdari Sam , Javadi Hamid Haj Seyyed 
Published: August 2022

AbstractThe number of computing resources required for high‐volume applications is ever‐increasing. As far as the users' requests generate workload variations over time and the resources of cloud servers that respond to the requests are limited, the quality of services (QoS) of the cloud server is experiencing fluctuations. The cloud federation is a place where the cloud service providers work together to overcome the problem of resource scarcity and poor service quality. As a result, the main aim of forming a cloud federation is to create an optimal one in a way that provides the required resources and QoS for customers and maximum profit for the providers. Since the service providers play a key role in cloud federation formation, and their absence deteriorates the QoS of the federation, it is required to form an optimal cloud federation that provides the best profit for service providers to encourage them to remain in the federation. In the present paper, first, a model will be introduced for evaluating the cloud service providers, inspired by the concepts of macroeconomics. Then a greedy algorithm will be presented for forming an optimal federation. The proposed algorithm uses the evaluation results to rank the service providers and selects the best federation based on this ranking. In addition, the reputation of providers is evaluated based on the proposed method, which is a criterion for introducing the best providers. Furthermore, the surplus shared resources of the providers will be determined using the evaluation results and its optimal value will be proposed to cloud service providers using the proposed method. Finally, a set of tests will be performed on the introduced system.
Immunomodulatory activity of mannosylglycerate and two unnatural mannosyl‐oligosaccharides obtained from microbial fermentation on RAW264.7 macrophages
Authors: Liu Juanjuan , Yang Jiangang , Zeng Yan , Tian Chaoyu , Men Yan , Sun Yuanxia 
Published: August 2022

AbstractMannosyl compounds have been widely used in nutrition, fodder, and vaccine adjuvant industries. In our previous study, the engineered strains for the biosynthesis of three mannosyl compounds including β‐1,2‐mannobiose (M2‐β‐1,2), β‐1,2‐mannotriose (M3‐β‐1,2), and mannosylglycerate (MG) have been developed. However, their biological activities have not been reported. Here, those three compounds were successfully purified after fermentation of the engineered strains, and their potential immunomodulatory activities on RAW264.7 macrophages were investigated with commercialized β‐1,4‐mannotriose (M3‐β‐1,4) as control. Our results showed that M3‐β‐1,2 and MG promoted the viability and phagocytic function of RAW264.7. Meanwhile, the cytokine TNF‐α and interleukin‐6 (IL‐6) level of RAW264.7 macrophages were significantly enhanced upon the stimulation of M3‐β‐1,2 and MG compared with M3‐β‐1,4. Moreover, MG significantly stimulated macrophages to secrete IL‐10 compared with other mannan oligosaccharides. Finally, this study proved that the immunomodulatory activity of M3‐β‐1,2 and MG on RAW 264.7 cells was mainly through mitogen‐activated protein kinases and myeloid differentiation protein 88 (MyD88)‐dependent signaling pathways. All these findings suggested that M3‐β‐1,2 and MG exhibited immunomodulatory activities in the innate and adaptive immune systems, thus facilitating the application potential in developing of mannosyl compounds as an immunomodulator available for food and pharmaceutical area.
Loss of ALK hotspot mutations in relapsed neuroblastoma
Authors: Allinson Lisa M. , Potts Aaron , Goodman Angharad , Bown Nick , Bashton Matthew , Thompson Dean , Basta Nermine O. , Gabriel Alem S. , McCorkindale Michael , Ng Antony , McNally Richard J. Q. , Tweddle Deborah A. 
Published: August 2022

AbstractALK is the most commonly mutated oncogene in neuroblastoma with increased mutation frequency reported at relapse. Here we report the loss of an ALK mutation in two patients at relapse and a paired neuroblastoma cell line at relapse. ALK detection methods including Sanger sequencing, targeted next‐generation sequencing and a new ALK Agena MassARRAY technique were used to detect common hotspot ALK variants in tumors at diagnosis and relapse from two high‐risk neuroblastoma patients. Copy number analysis including single nucleotide polymorphism array and array comparative genomic hybridization confirmed adequate tumor cell content in DNA used for mutation testing. Case 1 presented with an ALK F1174L mutation at diagnosis with a variant allele frequency (VAF) ranging between 23.5% and 28.5%, but the mutation was undetectable at relapse. Case 2 presented with an ALK R1257Q mutation at diagnosis (VAF = 39%–47.4%) which decreased to <0.01% at relapse. Segmental chromosomal aberrations were maintained between diagnosis and relapse confirming sufficient tumor cell content for mutation detection. The diagnostic SKNBE1n cell line harbors an ALK F1174S mutation, which was lost in the relapsed SKNBE2c cell line. To our knowledge, these are the first reported cases of loss of ALK mutations at relapse in neuroblastoma in the absence of ALK inhibitor therapy, reflecting intra‐tumoral spatial and temporal heterogeneity. As ALK inhibitors are increasingly used in the treatment of refractory/relapsed neuroblastoma, our study highlights the importance of confirming whether an ALK mutation detected at diagnosis is still present in clones leading to relapse.
Bird species potentially exposed to pesticide-treated corn seeds in the continental United States
Authors: Sullivan Joseph P. , Wisk Joseph D. 
Published: August 2022

Abstract
In the United States, the use of pesticide-coated seeds in agriculture is a widely adopted practice. Consumption of treated crop seeds is a possible route of exposure of birds to pesticides. A combination of agronomic and bird life history information is used to identify those species most likely to consume freshly planted corn seeds. Species listed as threatened or endangered by the US Fish and Wildlife Service that could consume treated seeds are also identified. Corn-growing counties in the continental United States were compiled for the years 2017–2020. Seven hundred and eight bird species occur in corn-growing counties. Feeding habits and habitats of North American bird species that occur in corn-growing counties indicate that 107 species could feed on the ground in or adjacent to freshly planted corn fields and consume seeds. Of these, 21 species show a high tendency to feed in or around freshly planted corn fields and could consume a large quantity of corn seeds. Of the 107 species with the potential to consume freshly planted corn, only two full species (as opposed to subspecies), the Gunnison sage-grouse and whooping crane, are listed as threatened or endangered under the US Endangered Species Act. Additionally, two subspecies, Mississippi sandhill crane and masked bobwhite, are listed as endangered. The streaked horned lark is listed as threatened. Of the 21 species, including subspecies, that have the greatest potential to consume a freshly planted treated corn seed, the Mississippi sandhill crane is the only listed species considered to have a high potential to consume treated corn seeds. Therefore, the potential exposure of federally listed threatened or endangered bird species to pesticides through the direct consumption of pesticide-treated corn seeds is low, with the possible exception of the Mississippi sandhill crane. Integr Environ Assess Manag 2023;19:461–473. © 2022 SETAC
Time intervals in the care pathway to cancer diagnosis during the COVID‐19 pandemic: A large retrospective study from a high‐volume center
Authors: Bosch Xavier , Montori‐Palacin Elisabet , Martínez‐Ferrer Rosa , Aldea Anna , Moreno Pedro , López‐Soto Alfonso 
Published: August 2022

AbstractDespite extensive research on cancer care during the COVID‐19 pandemic, evidence on the impact on prediagnostic time intervals is lacking. To better understand how COVID‐19 changed the pathway to diagnosis of cancer, we examined the length of intervals from symptom onset to diagnosis for 13 common cancer types with known clinical stage over 1‐year nonpandemic period (March 2019 to March 2020; N = 844) and three biannual COVID periods (March 2020 to September 2021; N = 1172). We analyzed the patient interval (from first symptoms to presentation to a physician), the primary care/emergency department interval (from presentation with relevant symptoms to a primary care or emergency department physician to referral to a hospital‐based diagnosis center) and the hospital interval (from referral to diagnosis). Compared to nonpandemic data, there were significant changes across COVID periods. The pandemic mostly impacted patient intervals for cancers diagnosed over the first 6 months after onset in March 2020. Overall median patient intervals were longest in the early COVID period (39 [IQR 22‐64] days) and shortest in the nonpandemic period (20 [IQR 13‐30] days; Kruskal‐Wallis test [χ2], P < .0001). Differences in clinical stage between periods were relevant, with cancers from the mid‐period (September 2020 to March 2021) showing the most advanced stage. A shift to later stage was plausibly a result of delayed intervals in the early COVID period. Since intervals are eventually relevant to prognosis, our results provide a baseline against which the impact of improvement strategies to minimize the negative outcomes of COVID‐19‐associated cancer delays can be assessed and implemented.
A novel serum extracellular vesicle protein signature to monitor glioblastoma tumor progression
Authors: Tzaridis Theophilos , Weller Johannes , Bachurski Daniel , Shakeri Farhad , Schaub Christina , Hau Peter , Buness Andreas , Schlegel Uwe , Steinbach Joachim‐Peter , Seidel Clemens , Goldbrunner Roland , Schäfer Niklas , Wechsler‐Reya Robert J. , Hallek Michael , Scheffler Björn , Glas Martin , Haeberle Lothar , Herrlinger Ulrich , Coch Christoph , Reiners Katrin S. , Hartmann Gunther 
Published: August 2022

AbstractDetection of tumor progression in patients with glioblastoma remains a major challenge. Extracellular vesicles (EVs) are potential biomarkers and can be detected in the blood of patients with glioblastoma. In our study, we evaluated the potential of serum‐derived EVs from glioblastoma patients to serve as biomarker for tumor progression. EVs from serum of glioblastoma patients and healthy volunteers were separated by size exclusion chromatography and ultracentrifugation. EV markers were defined by using a proximity‐extension assay and bead‐based flow cytometry. Tumor progression was defined according to modified RANO criteria. EVs from the serum of glioblastoma patients (n = 67) showed an upregulation of CD29, CD44, CD81, CD146, C1QA and histone H3 as compared to serum EVs from healthy volunteers (P value range: <.0001 to .08). For two independent cohorts of glioblastoma patients, we noted upregulation of C1QA, CD44 and histone H3 upon tumor progression, but not in patients with stable disease. In a multivariable logistic regression analysis, a combination of CD29, CD44, CD81, C1QA and histone H3 correlated with RANO‐defined tumor progression with an AUC of 0.76. Measurement of CD29, CD44, CD81, C1QA and histone H3 in serum‐derived EVs of glioblastoma patients, along with standard MRI assessment, has the potential to improve detection of true tumor progression and thus could be a useful biomarker for clinical decision making.
Depletion of Foxp3+ regulatory T cells but not the absence of CD19+IL‐10+ regulatory B cells hinders tumor growth in a para‐orthotopic neuroblastoma mouse model
Authors: Weißenborn Christine , von Lenthe Sophie , Hinz Nicole , Langwisch Stefanie , Busse Mandy , Schumacher Anne , Zenclussen Ana C. , Fest Stefan 
Published: August 2022

AbstractAdaptive immune cells with regulatory function reportedly mediate immune escape in a variety of tumors. Little is known regarding the relevance of the most prominent regulatory cell populations, namely Foxp3+ T regulatory cells (Tregs) and CD19+IL‐10+ B regulatory cells (Bregs), for neuroblastoma (NB) survival. After establishing a novel immunocompetent syngeneic NB mouse model where orthotopic tumors can be generated after intrarenal injection of NB975A cells, we studied the importance of Tregs and Bregs in Foxp3‐DTR mice whose Tregs can be depleted by diphtheria toxin (DT) application as well as in CD19‐specific IL‐10 deficient mice that lack IL‐10+ Bregs (CD19cre+/− × IL‐10fl/fl mice). We observed Foxp3 Treg cells in tumors from wild type mice. On the contrary, Bregs or B cells were scarce. Specific depletion of Tregs in Foxp3‐DTR mice resulted in an 85% reduction of tumor volume and weight compared to DT‐treated wild type mice and untreated Foxp3‐DTR mice. In contrast, NB tumor growth was not affected in CD19‐specific IL‐10 deficient mice. Similarly, mice lacking mature B cells (μMT mice) and CD19 deficient mice (CD19cre mice) showed no change in growth pattern of NB tumors. In Treg‐depleted mice, reduced tumor growth was associated with an increased concentration of IFN‐gamma, TNF‐alpha, IL‐4, IL‐6, and IL‐10 in isolated splenocytes. In summary, transient ablation of Tregs but not absence of Bregs hindered the growth of NB, strongly suggesting the therapeutic potential of targeting Tregs for this aggressive childhood tumor.
Dynamic variations of the gastric microbiota: Key therapeutic points in the reversal of Correa's cascade
Authors: Xi Jiahui , Li Yonghong , Zhang Hui , Bai Zhongtian 
Published: August 2022

AbstractCorrea's cascade is a dynamic process in the development of intestinal‐type gastric cancer (GC), and its pathological features, gastric microbiota and interactions between microorganisms and their hosts vary at different developmental stages. The characteristics of cells, tissues and gastric microbiota before or after key therapeutic points are critical for monitoring malignant transformation and early tumour reversal. This review summarises the pathological features of gastric mucosa, characteristics of gastric microbiota, specific microbial markers, microbe‐microbe interactions and microbe‐host interactions at different stages in Correa's cascade. The markers related to each Correa's cascade point were analysed in detail. We attempted to identify key therapeutic points for early cancer reversal and provide a novel approach to reduce the incidence of GC and improve precise treatment.
Quantitative proteomics identifies TUBB6 as a biomarker of muscle‐invasion and poor prognosis in bladder cancer
Authors: Kim Bohyun , Jung Minsun , Moon Kyung Chul , Han Dohyun , Kim Kwangsoo , Kim Hyeyoon , Yang Sunah , Lee Dongjoo , Jun Hyeji , Lee Kyung‐Min , Lee Cheng Hyun , Nikas Ilias P. , Yang Sohyeon , Lee Hyebin , Ryu Han Suk 
Published: August 2022

AbstractMuscle‐invasive urothelial carcinoma (MIUC) of the bladder shows highly aggressive tumor behavior, which has prompted the quest for robust biomarkers predicting invasion. To discover such biomarkers, we first employed high‐throughput proteomic method and analyzed tissue biopsy cohorts from patients with bladder urothelial carcinoma (BUC), stratifying them according to their pT stage. Candidate biomarkers were selected through bioinformatic analysis, followed by validation. The latter comprised 2D and 3D invasion and migration assays, also a selection of external public datasets to evaluate mRNA expression and an in‐house patient‐derived tissue microarray (TMA) cohort to evaluate protein expression with immunohistochemistry (IHC). Our multilayered platform‐based analysis identified tubulin beta 6 class V (TUBB6) as a promising prognostic biomarker predicting MIUC of the bladder. The in vitro 2D and 3D migration and invasion assays consistently showed that inhibition of TUBB6 mRNA significantly reduced cell migration and invasion ability in two BUC cell lines with aggressive phenotype (TUBB6 migration, P = .0509 and P < .0001; invasion, P = .0002 and P = .0044; TGFBI migration, P = .0214 and P = .0026; invasion, P < .0001 and P = .0001; T24 and J82, respectively). Validation through multiple public datasets, including The Cancer Genome Atlas (TCGA) and selected GSE (Genomic Spatial Event) databases, confirmed TUBB6 as a potential biomarker predicting MIUC. Further protein‐based validation with our TMA cohort revealed concordant results, highlighting the clinical implication of TUBB6 expression in BUC patients (overall survival: P < .001). We propose TUBB6 as a novel IHC biomarker to predict invasion and poor prognosis, also select the optimal treatment in BUC patients.
Physical activity and the risk of non‐Hodgkin lymphoma subtypes: A pooled analysis
Authors: Boyle Terry , Kleinstern Geffen , Bracci Paige M. , Cerhan James R. , Benavente Yolanda , Casabonne Delphine , Chiu Brian C.‐H. , Habermann Thomas M. , Holly Elizabeth A. , Liebow Mark , Norman Aaron , Paltiel Ora , Robinson Dennis , Rothman Nathaniel , Abu Seir Rania , Slager Susan L. , Villeneuve Paul J. , Wang Sophia S. , Weisenburger Dennis D. , Spinelli John J. 
Published: August 2022

AbstractNon‐Hodgkin lymphoma (NHL) is composed of a heterogeneous collection of subtypes with considerable differences in genetics, biology and aetiology. Studies to date on physical activity and NHL risk have not had sufficient sample size to evaluate whether associations differ by subtype. We pooled data from nine case‐control studies to examine the association between moderate‐to‐vigorous intensity physical activity (MVPA) and risk of NHL overall and by subtype (diffuse large B‐cell lymphoma, follicular lymphoma, chronic lymphocytic leukaemia/small lymphocytic lymphoma, marginal zone lymphoma and mature T‐cell lymphoma). A total of 5653 cases and 9115 controls were included in the pooled analysis. Physical activity was harmonised across nine studies and modelled as study‐specific tertiles. Multinomial logistic regression was used to estimate the association between physical activity and NHL, adjusting for confounders. The overall odds of NHL was 13% lower among participants in the most active tertile of MVPA compared to the least active tertile (adjusted odds ratio = 0.87, 95% CI = 0.80, 0.95). Similar decreases were observed across NHL subtypes. In summary, in this pooled analysis of case‐control studies, physical activity was associated with a modest risk reduction for each NHL subtype examined and with overall NHL.