bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification of the GST gene family and its expression pattern analysis under cold stress in quinoa (Chenopodium quinoa Willd.)
Authors: Zhou Guangyi , Li Kuiyin , Wang Rui , Liu Xiaojuan , Qin Xianjiao , Jian Zilin , Lu Bingyun , He Fang , Ren Mingjian 
Published: December 2022

Abstract
Background
Glutathione S-transferase (GST) is an antioxidant enzyme essential for cell protection because of its scavenging of reactive oxygen species accumulated under various stresses. Cold stress studies on the GST gene family have been conducted in several dicotyledonous and monocotyledonous plants, including Arabidopsis, rice, sweet potato, cantaloupe, and pumpkin. However, no relevant studies have been conducted on quinoa to date.
Results
In the present study, 59 GST (CqGST) genes were identified in the C. quinoa genome, among which 34 were located in the cytoplasm, 20 in the chloroplasts, and five in the ribosomes. Our phylogenetic analysis of CqGST and GST genes from Arabidopsis and rice showed that these genes were clustered into eight subfamilies, namely Tau, Phi, GHR, Zeta, Lambda, EF1B, DHER, and TCHQD. A total of 59 CqGSTs were located on 14 chromosomes, and none were located on chromosomes 00, 4, 9, 13, and 15. Eleven pairs of tandem-duplicated genes and 12 pairs of segmentally duplicated genes were identified in the CqGST gene family. The promoter region of each CqGST contained at least one cis-element associated with adversity. We selected 16 representative genes for fluorescence quantitative RT-PCR to verify gene expression and found that most of the CqGST genes were highly expressed in the roots and recovered for 3 h after different cold treatment times, indicating that the GST family plays an important role in quinoa cold stress.
Conclusions
In the present study, 59 GST genes were identified in quinoa, and gene duplication events were found to be the main drivers of GST gene family evolution in this species. Our results provide a basis for further studies on the function of GST genes in quinoa as well as a research basis for breeding quinoa in high-altitude cold regions, indicating the candidate genes for enhancing quinoa yield.
Characterization of Particulate Matter From the Chimney of a Former Primary Lead Smelter Using Chemical, Physical, and Mineralogical Methods
Authors: Lima Luiz Rogério Pinho de Andrade , Bernardez Letícia Alonso 
Published: December 2022

Abstract
There are recent allegations of kids residing close to a lead smelter, which shut down in 1993, who have high Cd levels in their blood. The study’s goal is to identify the source of Cd pollution and other potentially harmful elements, with a particular emphasis on the chimney dust, which were used for this characterization investigation. Chemical, physical and mineralogical methods were used in this investigation. The principal phases in the dust are: sulfur, anglesite and kaolin. After sulfur removal the chemical analysis revealed that the following elements constitute the majority of the dust (wt%): 10.4% of S, 44.37% of PbO, 17.34% of SiO2, 6.28% of Al2O3, 1.22% of ZnO, 0.93% of As2O3. The amount of Hg was 4670 mg/kg and the amount of Cd was higher than 5000 mg/kg. Anglesite was highly concentrated in the dust. According to the leaching investigation, the chimney dust was unstable in mildly acidic conditions for only brief contact durations, releasing significant amounts of Pb, Zn, and Sb as well as a significant quantity of Cd. Therefore, given the toxic elements, this dust should be considered a very hazardous material.
Hierarchically Multifunctional Thermally Conductive Boron Nitride/Polyurethane Fibrous Membranes via Constructing Alternating Multi-Layer Orientation Structure
Authors: Jiang Fang , Li Yutao , Ye Minghua , Chen Xiaolong , Shen Yifeng , Ding Peng , Yang Lei 
Published: December 2022

Abstract

Thermally conductive polymeric materials with versatility are in high demand in electronic devices. Whereas, the limited thermal conductivity and versatility restrict the development of such materials. In this work, the hierarchical design and assembly technology was applied to prepare hierarchically multifunctional functionalized boron nitride/polyurethane multi-layer (m-FBN/PU) fibrous membranes with higher quality and higher quantity of oriented thermally conductive network. FBN/PU filler layers act as thermally conductively pathways and PU substrate layers provide mechanical support and the obtained hydrophobic and flexible m-FBN/PU fibrous membranes with 10 wt% FBN filling display high in-plane thermal conductivity (5.78 W·m− 1·K− 1) and electrical insulation (1014 Ω·cm). Meanwhile, in comparison with the FBN/PU single-layer (s-FBN/PU) fibrous membranes with the same filler content, the thermal conductive enhancement reaches 61.5%, which could be ascribed to the increased phonon transfer network built by the alternated aligned hierarchical structure. Our fabrication provides a promising strategy for developing multifunctional polymeric materials and has the potential in advanced electronics and emerging fields like wearable electronics.
Application of the Consolidated Framework for Implementation Research to Inform Understanding of Barriers &amp; Facilitators to the Implementation of Opioid and Naloxone Training on College Campuses
Authors: Shelton Rachel C. , Goodwin Kathleen , McNeil Michael , Bernitz Melanie , Alexander Savannah P. , Parish Carrigan , Brotzman Laura , Lee Matthew , Li WaiKwan (Bonnie) , Makam Supriya , Ganek Nicholas , Foskett Dean , Warren Chloe , Metsch Lisa R. 
Published: December 2022

Abstract
Background: The opioid epidemic in the United States continues to worsen. Opioid-only and polysubstance-involved opioid overdose deaths are increasing among adolescents and young adults, who have limited knowledge of opioid overdose prevention, including recognition and response. College campuses have infrastructure to support national-level implementation of evidence-based public health strategies for providing opioid overdose prevention and naloxone training programs among this priority population. However, college campuses are an underutilized, understudied setting for this programming. To address this gap, we conducted research assessing barriers and facilitators to planning and implementing this programming on college campuses.
Methods: We held 9 focus groups among purposively selected campus stakeholders whose perceptions were important to understand in planning for the dissemination and implementation of opioid overdose prevention and naloxone training. Focus group scripts were informed by The Consolidated Framework for Implementation Research (CFIR) to query about perceptions of opioid and other substance use, opioid and other substance use-related resources, and naloxone administration training. We used a deductive-inductive, iterative approach to thematic analysis.
Results: Themes about implementation barriers included: 1) the perception that problematic use of other (non-opioid) substances was more prevalent than opioid use on campus and focus on those substances would be a greater priority on college campuses; 2) student schedules were overwhelmed with academic commitments and extracurricular activities, making delivery of additional training challenging; 3) barriers related to the perceived complexity and decentralization of communication on campus, preventing students from knowing how to access substance use-related resources. Themes about implementation facilitators included: 1) framing naloxone training as important in becoming a responsible leader on campus and in the broader community; 2) leveraging existing infrastructure, champions within existing campus groups, and tailored messaging to facilitate participation in naloxone training.
Conclusions: This is the first study to provide in-depth insights into potential barriers and facilitators to widespread, routine implementation of naloxone/opioid education on undergraduate college campuses. The study captured diverse stakeholder perspectives and was theoretically grounded in CFIR, contributing to the growing literature on the application and refinement of CFIR across diverse community and school contexts.
Study on Thermal Insulation Cement and Its Thermal Insulation Charateristics for Geothermal Wells
Authors: Zhou Wei , Wang Chengwen , Meng Renzhou , Chen Zehua , Lu Haoxin , Chi Jialun 
Published: December 2022

Abstract
Geothermal energy is a clean, efficient and plentiful unconventional energy, and reducing the heat loss in wellbore is the key for efficient development of geothermal resource. It is a reliable solution to establish a long-term stable wellbore with good thermal insulation effect through cementing, which requires hardened cement with good mechanical and thermal insulation properties. However, there is little research in this regard at present. In this paper, a multi-material and multi-structure hollow cement slurry is developed, and a thermal-conductivity prediction method is proposed correspondingly based on Maxwell model. Nitrogen-filled foam and hollow glass beads were employed to reduce the heat conduction path, and their effects on the thermal conductivity, mechanical properties of hardened cement are analyzed. Results shows that: the distribution of self-generating nitrogen foam in foamed cement is uniform and stable, which is benefit for the mechanical strength and thermal insulation effect of hardened cement; 10 ~ 13% hollow glass beads can significantly reduce the thermal conductivity of hardened cement, without significant adverse effects on the rheology and strength of foamed cement; The prediction error of the newly established model is within 2%, which can be used to guide the optimization of thermal insulation cement. This research can provide technical support for efficient development of geothermal resources.
Intelligent Eucommia ulmoides rubber/ionomer composites with thermally activated shape memory and self-healing behaviors
Authors: Wang Qi , Li Yutao , Xiao Jianbin , Xia Lin 
Published: December 2022

Abstract
Intelligent Eucommia ulmoides rubber (EUR) and ionomer Surlyn resin (SR) composites were prepared and studied in this manuscript. This is the first paper to combine EUR with SR to prepare composites with both the shape memory effect and self-healing capability. The mechanical, curing, thermal, shape memory and self-healing properties were studied by a universal testing machine, differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA), respectively. Experimental results showed that the increase in ionomer content not only improved mechanical and shape memory properties but also endowed the compounds with excellent self-healing ability under appropriate environmental conditions. In particular, the self-healing efficiency of the composites reached 87.41%, which was significantly higher than that of the other composites. Therefore, these novel shape memory and self-healing composites can expand the use of natural Eucommia ulmoidesrubber, such as in special medical devices, sensors and actuators.
Is panoramic radiography adequate for diagnosing coronoid process hyperplasia? A case series
Authors: Mattei Léa , Raoul Gwénaël , Barry Florent , Ferri Joël , Nicot Romain 
Published: December 2022

Abstract

Introduction: The objective of this study was to compare the length ratios obtained on panoramic radiography and computed tomography (CT) to verify whether the former is adequate for diagnosing coronoid process hyperplasia. Methods: A case series of patients with coronoid process hyperplasia was investigated. Length ratios between the coronoid process and condyle were measured on panoramic radiographs by using the Levandoski method and on CT scans by using the methods described by Tavassol et al. and Stopa et al. The mean length ratios obtained using the three measurement methods were compared. Results: The mean length ratio measured with the Levandoski method was significantly lower than that measured with the method described by Stopa et al. (1.09 [0.09] vs. 1.21 [0.09]; P = 0.0001) and lower than that measured with the method described by Tavassol et al. (1.09 [0.09] vs. 1.34 [0.44]; P = 0.013). Conclusion: Panoramic measurement of the coronoid process by using the Levandoski method tended to underestimate the length ratio, emphasizing the importance of using a scanographic measurement method at the slightest doubt to confirm the diagnosis of coronoid process hyperplasia.
Persistent symptoms after COVID-19 during the first wave are not associated with differential immunity to SARS-CoV-2
Authors: Altmann Daniel , Reynolds Catherine , Joy George , Otter Ashley , Gibbons Joseph , Pade Corinna , Swadling Leo , Maini Mala , Brooks Tim , Semper Amanda , McKnight Aine , Noursadeghi Mahdad , Manisty Charlotte , Treibel Thomas , Moon James , Boyton Rosemary 
Published: December 2022

Abstract
Among the unknowns in decoding the pathogenesis of SARS-CoV-2 persistent symptoms in Long Covid is whether there is a contributory role of abnormal immunity during acute infection - some have proposed that Long Covid may be a consequence of either an excessive or inadequate initial response. We analysed SARS-CoV-2 humoral and cellular immunity in healthcare workers infected during the first wave. Symptom questionnaires allowed stratification into those with persistent symptoms and those without for comparison. During the period up to 18-weeks post-infection, we observed no difference in antibody responses to spike, RBD or nucleoprotein, virus neutralisation, or T cell responses. Also, there was no difference in the profile of antibody waning. Analysis at 1-year, after two vaccine doses, comparing those with persistent symptoms to those without, again showed similar SARS-CoV-2 immunity. Thus, quantitative differences in SARS-CoV-2 adaptive immunity during acute infection are unlikely to contribute to Long Covid causality.
Efficacy of Ceftazidime in a Murine Model Following a Lethal Aerosol Exposure to Burkholderia pseudomallei
Authors: Pfefferle Denise A. , Hackett Michael , Anderson Michael S. , Gibbs Seth , Henning Lisa N. , Joice April C. , Meister Gabriel T. 
Published: December 2022

Abstract
Melioidosis is an endemic disease in numerous tropical regions. Additionally, the bacterium that causes melioidosis, Burkholderia pseudomallei, has potential to be used as a biological weapon. Therefore, development of effective and affordable medical countermeasures to serve regions affected by the disease and to have medical countermeasures available in the event of a bioterrorism attack remains critical. The current study evaluated the efficacy of eight distinct acute phase ceftazidime treatment regimens administered therapeutically in the murine model. At the conclusion of the treatment period, survival rates were significantly greater in several of the treated groups when compared to the control group. Pharmacokinetics of a single dose of ceftazidime were examined at 150 mg/kg, 300 mg/kg, and 600 mg/kg and were compared to an intravenous clinical dose administered at 2,000 mg every eight hours. The clinical dose has an estimated 100% fT > 4*MIC which exceeded the highest murine dose of 300 mg/kg every six hours at 87.2% fT > 4*MIC. Based upon survival at the end of the treatment regimen and supplemented by pharmacokinetic modeling, a daily dose of 1200 mg/kg of ceftazidime, administered every 6 hours at 300 mg/kg, provides protection in the acute phase of inhalation melioidosis in the murine model.
Absorption Kinetics of Monosaccharides, Disaccharides and its combination with its impact on glucose availability.
Authors: Prajapati Ramesh , Mohapatra Jogeswar , Sharma Manoranjan , Jha Abhishek , Patro Randeep , Dhar Shilpa , Chondhekar Pravin , Purohit Pratibha , Chatterjee Abhijit , Raghavan Govindarajan 
Published: December 2022

Abstract
Carbohydrates are the primary macronutrients that humans consume to support the body’s need for a source of energy. Monosaccharides like glucose are the simplest form of carbohydrate that is readily absorbed in the bloodstream, followed by disaccharides like sucrose, which are converted into glucose prior to absorption and energy release. To evaluate the absorption, distribution, and absorption kinetics of glucose, sucrose, and a combination of the two in the bloodstream, a preclinical study was conducted in Sprague-Dawley (SD) rats showing instant and sustained energy release through the change in blood glucose level after intake of the test product. The data of blood glucose tested using a glucometer were analyzed and evaluated using a regression method. The comparison study determines whether monosaccharides, disaccharides, or a blend of both have an impact on absorption kinetics and sustenance of blood glucose levels. The result showed glucose was absorbed in the bloodstream at a faster rate as early as 60 seconds (onset time) than sucrose and peaked within 30 minutes thus providing instant energy. Additionally, sucrose and its combination with glucose maintained blood glucose levels for a longer duration compared to glucose alone with a lower regression slope of 0.744 (sucrose) vs. 1.247 (glucose). Sucrose and its combination with glucose providing slow and steady energy release upto 4 hours. In conclusion, glucose provided instant energy, while sucrose and its combination with glucose provided sustained energy release after intake.
Optimization of Mucilage Extrusion of Plantago, Asylum, and Basil Seeds by Response Surface Method
Authors: Tafaghodinia Bahram 
Published: December 2022

Abstract
The set of seed coats and other organs that play a role in protecting the embryo and helping to disperse is called the diaspore. The pericarp secretes a gelatinous mixture of cell wall polysaccharides called mucilage in many species. The synthesis, secretion, and modification of the mucilage strain have extensively studied the structure and function of the plant cell wall. However, efforts to understand the best condition for mucilage extrusion have received less attention. In general, mucilage appears to supply the water needed for seed growth, protect the seeds in the intestines of animals when eaten, or affect the ability of the seeds to sink or float in the water and disperse the seeds. However, the main role of this study was to investigate the best condition of holding capacity of mucilage seeds of Plantago, Basil, and Asylum. The effect of polarity, grain number, species, and pH on mucilage extrusion was investigated. Data analysis showed that the number of grains had the least and the type of species had the most impact on the amount of mucilage produced. Overall, the results of the response surface design experiments confirmed the significant effect of pH, polarity, and grain species on the mucilage secretion process. The optimal conditions for the secretion of 10.3 mm of Plantago mucilage, 9.4 mm of Basil mucilage, and 2.9 mm of Asylum mucilage were pH=4 and polarity=15, pH=10 and polarity=15, pH=10 and polarity=15, respectively. Also, the water retention capacity of the mucilage of these seeds was investigated. The results showed that the outer shell of Plantago, Asylum, and Basil seeds have a water retention capacity of 56, 78, and 121 times their weight, respectively.
Circ_0001451 inhibits gastric cancer metastasis via miR-197-3p /MT3 axis
Authors: Liao Jinrong , Chen Zeng , Lin Xinyi , Lu Liancheng , Huang Zhiyi , Su Ying , Zou Changyan , Zheng Qianlan , Zhang Lurong , Huang Tao , Lin Xiandong 
Published: December 2022

Abstract
Background
CircRNAs, new non-coding RNA as miRNA sponge, play crucial roles in tumor initiation and development. The present study aimed to identify hsa_circ_0001451 and its related molecules for the progression of gastric cancer.
Methods
The expression of hsa_circ_0001451 was determined in 66 cases of paired gastric cancer and their adjacent normal gastric tissues by qRT-PCR. The cellular localization of hsa_circ_0001451 was detected with FISH analysis. The biofunction alterations of hsa_circ_0001451 were assessed by its overexpression or knockdown in gastric cancer cell lines. MTS and cloning assay were used to detect cell proliferation. Flow cytometry was used to detect cell cycle. The wound healing and transwell assays were used for cell migration and invasion. Western Blotting was used to detect the expression of a set of genes related proliferation and migration. RNA immunoprecipitation (RIP), biotinylated RNA pull-down and dual luciferase reporter tests were used to detect the interactions of hsa_circ_0001451with mir-197-3p / MT3. The in vivo proliferation and migration ability of hsa_circ_0001451infected cells were determined in zebrafish system.
Results
Hsa_circ_0001451 was decreased in 66 cases of gastric cancer tissues and cell lines, and significantly related to tumor invasion. The in vitro and in vivo studies indicated that over-expression of hsa_circ_0001451 inhibited the gastric cancer cell proliferation, invasion and migration via suppression of PCNA, CCND1 and BCL-2, and decreased in the S-phase. The results of pull-down, RNA immunoprecipitation and dual-luciferase reporter tests revealed that hsa_circ_0001451 could sponge miR-197-3p, targeting MT3 and affecting the malignancy of gastric cancer cells via regulating EMT related E-cadherin, N-cadherin, MMP7 and VIMENTIN.
Conclusion
This study revealed that hsa-circ-0001451 inhibited proliferation, invasion and metastasis and modulated EMT of gastric cancer cells via sponging of miR-197-3p and regulatng the downstream MT3. The circ_0001451 / miR-197-3p / MT3 axis are likely to be the biomarkers for prognosis and targets for drug of gastric cancer.
CdS/MIL-101 as a Visible-light-driven Photocatalyst for Methylene Blue Degradation
Authors: Dang Anh , Doan Tuan , Huyen Pham Thanh 
Published: December 2022

Abstract
This research presents the hydrothermal synthesis of the metal-organic framework MIL-101 (Cr) and the CdS/MIL-101 (Cr) catalyst in the photocatalytic degradation of Methylene Blue (MB) dye. X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Scanning/transmission electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscope (TEM), thermal analysis (TG), UV-Vis diffuse reflectance spectroscopy (UV-Vis DRS), and nitrogen adsorption/desorption isotherms at 77K were used to characterize the produced samples. Obtained MIL-101 had high crystallinity and a specific surface area of 2,980m2/g. The catalytic activities of CdS/MIL-101 were determined for the degradation of MB in an aqueous solution under visible light. The results demonstrate that CdS/MIL-101 was a good heterogeneous photocatalyst for the degradation of MB. The reactive species of ·OH, e–, and h + generated on CdS were verified to play the predominant role and were believed to be the mechanism of photocatalysis. Using the initial rate approach and the Langmuir-Hinshelwood model, the team investigated the kinetics of the photocatalytic degradation reaction. The CdS/MIL-101 photocatalyst had very good catalytic recyclability and stability, which makes it a possible catalyst for cleaning organic pollutants in water.