bluuebunny/crossref_metadata_2025_split huggingface dataset:
8-HQA Adjusts the Number and Diversity of Bacteria in the Gut Microbiome ofSpodoptera littoralis
Authors: Mazumdar Tilottama , Hänniger Sabine , Shukla Shantanu P. , Murali Aishwarya , Bartram Stefan , Heckel David G. , Boland Wilhelm 
Published: December 2022

AbstractQuinolinic carboxylic acids are known for their metal ion chelating properties in insects, plants and bacteria. The larval stages of the lepidopteran pest,Spodoptera littoralis, produce 8-hydroxyquinoline-2-carboxylic acid (8-HQA) in high concentrations from tryptophan in the diet. At the same time, the larval midgut is known to harbor a bacterial population. The motivation behind the work was to investigate whether 8-HQA is controlling the bacterial community in the gut by regulating the concentration of metal ions. Knocking out the gene for kynurenine 3-monooxygenase (KMO) in the insect using CRISPR/Cas9 eliminated production of 8-HQA and significantly increased bacterial numbers and diversity in the larval midgut. Adding 8-HQA to the diet of knockout larvae caused a dose-dependent reduction of bacterial numbers with minimal effects on diversity.Enterococcus mundtiidominates the community in all treatments, probably due to its highly efficient iron uptake system and production of the colicin, mundticin. Thus host factors and bacterial properties interact to determine patterns of diversity and abundance in the insect midgut.
The E2 SUMO-conjugating enzyme UBE2I coordinates the oocyte and zygotic transcriptional programs
Authors: Briley Shawn M. , Ahmed Avery A. , Jiang Peixin , Hartig Sean M. , Schindler Karen , Pangas Stephanie A. 
Published: December 2022

AbstractIn mammals, meiotically competent oocytes develop cyclically during ovarian folliculogenesis. During folliculogenesis, prophase I arrested oocytes are transcriptionally active, producing and storing transcripts required for their growth and for early stages of embryogenesis prior to the maternal to zygotic transition. Defective oocyte development during folliculogenesis leads to meiotic defects, aneuploidy, follicular atresia, or non-viable embryos. Here we generated a novel oocyte-specific knockout of the SUMO E2 ligase,Ube2i, usingZp3-creto test its function during folliculogenesis.Ube2i Zp3-cre+ female mice are sterile with oocytes that arrest in meiosis I with defective spindles and chromosome alignment. Fully grown mutant oocytes abnormally maintain transcription but downregulate maternal effect genes and prematurely activate the zygotic transcriptional program. Thus, this work uncovers UBE2i as a novel orchestrator of chromatin and transcriptional regulation in mouse oocytes.TeaserOocyte-specific deletion ofUbe2icauses loss of transcriptional repression and premature activation of the zygotic genome.
Itch receptor MRGPRX4 interacts with the receptor activity-modifying proteins (RAMPs)
Authors: Kotliar Ilana B. , Ceraudo Emilie , Kemelmakher-Liben Kevin , Oren Deena A. , Lorenzen Emily , Dodig-Crnković Tea , Horioka-Duplix Mizuho , Huber Thomas , Schwenk Jochen M. , Sakmar Thomas P. 
Published: December 2022

AbstractCholestatic itch is a severe and debilitating symptom in liver diseases with limited treatment options. The class A G protein-coupled receptor (GPCR) Mas-related GPCR subtype X4 (MRGPRX4) has been identified as a receptor for bile acids, which are potential cholestatic pruritogens. An increasing number of GPCRs have been shown to interact with receptor activity-modifying proteins (RAMPs), which can modulate different aspects of GPCR biology. Using a combination of multiplexed immunoassay and proximity ligation assay we show that MRGPRX4 interacts with RAMPs. The interaction of MRGPRX4 with RAMP2, but not RAMP1 or 3, causes attenuation of basal and agonist-dependent signaling, which correlates with a decrease of MRGPRX4 cell surface expression as measured using a quantitative NanoBRET pulse-chase assay. Finally, we use AlphaFold Multimer to predict the structure of the MRGPRX4-RAMP2 complex. The discovery that RAMP2 regulates MRGPRX4 may have direct implications for future drug development for cholestatic itch.
Role of interneuron subtypes in controlling trial-by-trial output variability in the neocortex
Authors: Guo Lihao , Kumar Arvind 
Published: December 2022

AbstractTrial-by-trial variability is a ubiquitous property of neuronal activity in vivo and affects the stimulus response. Computational models have revealed how local network structure and feedforward inputs control the trial-by-trial variability. However, the role of input statistics and different interneuron subtypes in shaping the trial-by-trial variability was less understood. Here we investigated the dynamics of stimulus response in a model of cortical microcircuit with one excitatory and three inhibitory interneuron populations (PV, SST, VIP). We show that the variance ratio of inputs to different neuron populations and input covariances are the main determinants of output trial-by-trial variability. The effect of input covariances is contingent on the input variance ratios. In general, the network shows smaller output trial-by-trial variability in a PV-dominated regime than in an SST-dominated regime. Our work reveals mechanisms by which output trial-by-trial variability can be controlled in a context, state, and task-dependent manner.
Pulse-chase experiments reveal dynamics of RNA binding protein Exuperantia inDrosophila melanogaster eggchambers
Authors: Vieira Diana V. , Carlota Rita R. , de-Carvalho Jorge , Telley Ivo A. 
Published: December 2022

AbstractIn cells, mRNA can be associated with various proteins, forming ribonucleoprotein complexes (RNPs) which take part in spatiotemporal control of translation. In theDrosophila melanogasterdeveloping egg chamber, a set of RNPs is transported from the nurse cells to the oocyte and targeted selectively to specific cellular locations. This mRNA sorting process leads to the final oocyte polarization pre-defining the body axes of the future embryo. However, how mRNA is encoded for selection and directed transport is mechanistically not well understood. A master mRNA involved in body axes formation isbicoid, which localizes anterolaterally and is essential for head and thorax definition of the embryo. A protein that was identified essential forbicoidanterior localization is Exuperantia (Exu). Here, we use a live imaging–based pulse-chase approach, which reveals selective transport dynamics of Exu from nurse cells to the oocyte during mid to late-stage oogenesis.
Unsupervised reference-free inference reveals unrecognized regulated transcriptomic complexity in human single cells
Authors: Dehghannasiri Roozbeh , Henderson George , Bierman Rob , Baharav Tavor , Chaung Kaitlin , Wang Peter , Salzman Julia 
Published: December 2022

AbstractMyriad mechanisms diversify the sequence content of eukaryotic transcripts at both the DNA and RNA levels, leading to profound functional consequences. Examples of this diversity include RNA splicing and V(D)J recombination. Currently, these mechanisms are detected using fragmented bioinformatic tools that require predefining a form of transcript diversification and rely on alignment to an incomplete reference genome, filtering out unaligned sequences, potentially crucial for novel discoveries. Here, we present SPLASH+, significantly advancing biological discovery possible with SPLASH, our recently introduced efficient, reference-free statistical approach. Integrating a micro-assembly and biological interpretation framework, SPLASH+ enables new discoveries including broad and novel examples of transcript diversification in single cellsde novo, without the need for cell type metadata, which is impossible with current algorithms. Applied to 10,326 primary human single cells across 19 tissues profiled with SmartSeq2, SPLASH+ discovers a set of splicing and histone regulators with highly conserved intronic regions that are themselves subject to complex splicing regulation. Additionally, it reveals unreported transcript diversity in the heat shock proteinHSP90AA1, as well as diversification in centromeric RNA expression, V(D)J recombination, RNA editing, and repeat expansion, all missed by existing methods. SPLASH+ is highly efficient, enabling the discovery of an unprecedented breadth of RNA regulation and diversification in single cells through a new automated paradigm of unbiased transcriptomic analysis.
Brain connectivity changes underlying depression and fatigue in relapsing-remitting multiple sclerosis: a systematic review
Authors: Kampaite Agniete , Gustafsson Rebecka , York Elizabeth N. , Foley Peter , MacDougall Niall J. J. , Bastin Mark E. , Chandran Siddharthan , Waldman Adam D. , Meijboom Rozanna 
Published: December 2022

AbstractMultiple Sclerosis (MS) is an autoimmune disease affecting the central nervous system, characterised by neuroinflammation and neurodegeneration. Fatigue and depression are common, debilitating, and intertwined symptoms in people with relapsing-remitting MS (pwRRMS). An increased understanding of brain changes and mechanisms underlying fatigue and depression in RRMS could lead to more effective interventions and enhancement of quality of life. To elucidate the relationship between depression and fatigue and brain connectivity in pwRRMS we conducted a systematic review.Searched databases were PubMed, Web-of-Science and Scopus. Inclusion criteria were: studied participants with RRMS and differentiated between MS subtypes; published between 2001-01-01 and 2023-01-18; used fatigue and depression assessments validated for MS; included brain structural, functional magnetic resonance imaging (fMRI) or diffusion MRI (dMRI). Sixty studies met the criteria: 18 dMRI (15 fatigue, 5 depression) and 22 fMRI (20 fatigue, 5 depression) studies.The literature was heterogeneous; half of studies reported no correlation between brain connectivity measures and fatigue or depression. Positive findings showed that abnormal cortico-limbic structural and functional connectivity was associated with depression. Fatigue was linked to connectivity measures in cortico-thalamic-basal-ganglial networks. Additionally, both depression and fatigue were related to altered cingulum structural connectivity, and functional connectivity involving thalamus, cerebellum, frontal lobe, ventral tegmental area, striatum, default mode and attention networks, and supramarginal, precentral, and postcentral gyri.Qualitative analysis suggests neuropathological effects, possibly due to axonal and/or myelin damage, in the cortico-thalamic-basal-ganglial and cortico-limbic network may underlie fatigue and depression in pwRRMS, respectively, but the overall results were inconclusive. This highlights the need for studies including advanced MRI to detect more subtle brain changes in association with depression and fatigue as functional and microstructural studies more consistently show correlations. Further studies using optimised imaging protocols and validated depression and fatigue measures are required to clarify the substrate underlying these symptoms in pwRRMS.
Transforming libraries into learning collaborative hubs: the current state of physical spaces and the perceptions of Greek librarians concerning implementation of the “Learning Commons” model
Authors: Garoufali Angeliki , Garoufallou Emmanouel 
Published: December 2022

Purpose
With the technological innovation dominating higher education, the university libraries, as physical spaces, continue to play a crucial role in connecting students with knowledge. The emergence of the “Learning Commons” serves as a catalyst in the design of library spaces, renewing libraries’ roles and missions and making them popular to Millennials for new reasons. This study aims to record Greek librarians' views on the current situation in Greek academic libraries regarding physical space design, services provided and the existence of the “Learning Commons” model characteristics.

Design/methodology/approach
This study was conducted through an online survey structured questionnaire (closed-ended, five-point Likert scale, multiple-choice and statements questions). This study population comprised librarians working in 37 academic institutions and colleges. The collected data were subjected to descriptive statistical analysis. The research questions were answered using variables creation and the tests, t-test, ANOVA and Kruskal–Wallis. The groups of questions were tested for their reliability using the Cronbach's alpha coefficient.

Findings
In total, 186 librarians responded to this study. The responses revealed that participants were willing and ready to accept and support a different approach to academic library physical spaces use, according to the shared learning spaces model. However, this requires changes in the organization's vision, further growth and evolution. Although recently most Greek academic libraries have made significant progress in developing their services to function as information and digital hubs, they do not function as learning collaborative hubs, since the “Learning Commons” model is not reflected in their buildings.

Originality/value
The creation of new academic libraries according to the “common” model is a rapidly evolving issue that affects Greek libraries. This paper highlights the characteristics of libraries that should be adopted in the modern era, the new roles of academic librarians and the importance of an appropriate design of the physical space to achieve optimal learning outcomes. At the same time, this paper is one of the few that illustrates librarians', and not users', perceptions of these changes. This paper is a good research example, and the methodology for measuring this type of context could be used by other future research approaches in other countries.
Towards the design and optimisation of future compact aero-engines: intake/fancowl trade-off investigation
Authors: Tejero Fernando , MacManus David , Matesanz García Jesús , Swarthout Avery , Sheaf Christopher 
Published: December 2022

Purpose
Relative to in-service aero-engines, the bypass ratio of future civil architectures may increase further. If traditional design rules are applied to these new configurations and the housing components are scaled, then it is expected that the overall weight, nacelle drag and the effects of aircraft integration will increase. For this reason, the next generation of civil turbofan engines may use compact nacelles to maximise the benefits from the new engine cycles. The purpose of this paper is to present a multi-level design and optimisation process for future civil aero-engines.

Design/methodology/approach
An initial set of multi-point, multi-objective optimisations for axisymmetric configurations are carried out to identify the trade-off between intake and fancowl bulk parameters of highlight radius and nacelle length on nacelle drag. Having identified the likely optimal part of the design space, a set of computationally expensive optimisations for three-dimensional non-axisymmetric configurations is performed. The process includes cruise- and windmilling-type operating conditions to ensure aerodynamic robustness of the downselected configurations.

Findings
Relative to a conventional aero-engine nacelle, the developed process yielded a compact aero-engine configuration with mid-cruise drag reduction of approximately 1.6% of the nominal standard net thrust.

Originality/value
The multi-point, multi-objective optimisation is carried out with a mixture of regression and classification functions to ensure aerodynamic robustness of the downselected configurations. The developed computational approach enables the optimisation of future civil aero-engine nacelles that target a reduction of the overall fuel consumption.
The use of a flipped classroom and experiential learning in an undergraduate management course
Authors: Lantu Donald Crestofel , Labdhagati Haifa , Bangun Yuni Ros , Sumarli Felicia Dwirahma 
Published: December 2022

PurposeThis paper reflects on a semester-long psychology and organizational behaviour course in an Indonesian business school. The course applied a flipped classroom (FC) approach combined with an experiential learning (EL) project that empowered students to work closely with local communities.Design/methodology/approachThis study compares students' final scores from an FC and EL class with students from other classes who used the traditional lecture method. Focus group interviews were conducted to gain insight into the students' experience.FindingsThe study results show that FC-EL students achieved higher final scores than traditional-learning students. The majority of students also reported having positive views regarding the approach, with a minority opposing the learning method. The students' projects were successfully implemented, empowered local communities and fostered reflexivity in the students.Research limitations/implicationsThe school has yet to implement FC and EL as course delivery methods in other courses. Therefore, the positive outcomes achieved in this study were obtained from only a single course.Originality/valueThe course combined flipped classroom and experiential learning methods.
Implementing Article 12 of the United Nations Convention on the Rights of the Child in health care: a scoping review
Authors: Davies Clare , Waters Donna , Fraser Jennifer Anne 
Published: December 2022

Purpose
The purpose of this paper is to present the results of a scoping review on the implementation of Article12 in health care. The scoping review will provide a summary and overview of the key concepts and published literature on this topic internationally. Article 12 of the United Nations Convention on the Rights of the Child (1989) states that children have a right to express their views, to have them heard and for their views to be given due weight in all matters that affect them. Despite increased calls for Article 12 to be given attention in health care, there is little evidence to suggest this has been well implemented and embedded in Australian health-care delivery. The scoping review was undertaken to provide a summary and overview of the key concepts and published literature on this topic internationally.

Design/methodology/approach
A five-step methodological framework described by Arksey and O’Malley (2005) was used to undertake the scoping review. Preferred Reporting Items for Systematic Reviews and Meta-Analysis was used as a guideline for undertaking the study selection.

Findings
Children are still not routinely involved in health-care decision-making, are frequently left out of service planning and evaluation and the perception that they lack the capability to make rational decisions persists.

Originality/value
While there has been a focus on research that investigates children’s participation in health-care decision-making in recent years, there is little that directs attention specifically to the implementation of Article 12, particularly in Australian health care. Recommendations are made for further research in these areas.
Top management’s green transformational leadership and competitive advantage: the mediating role of green organizational learning capability
Authors: Özgül Burcu , Zehir Cemal 
Published: December 2022

Purpose
This study aims to develop an original green organizational learning capability (GOLC) framework to examine the effects of green transformational leadership (GTL) on competitive advantage (CA) through GOLC by making use of the natural resource-based view (NRBV).

Design/methodology/approach
The current research proposes GOLC as a novel construct that simultaneously integrates green absorptive capability (GAC) and green transformative capability (GTC). Furthermore, this study presents a theoretical model that investigates GOLC as an intermediate mechanism in the relationship between GTL and CA based on the NRBV. The partial least squares method is used to test the data collected from 265 firms included in the list of Turkey’s Top 500 Industrial Enterprises in 2019 and having ISO 14001 certificate.

Findings
Top management’s GTL positively affects the firm’s GOLC. Moreover, GOLC positively affects the firm’s CA. This study further shows that GTL has a significant indirect effect on CA through GOLC.

Practical implications
This study demonstrates how firm managers can be persuasive in adopting GOLC with a critical role in developing and promoting green products and services to improve the firm’s environmental sustainability and CA by exhibiting GTL.

Originality/value
This research applies the NRBV theory to propose a novel concept, GOLC and develops an integral conceptual model to discover its managerial impacts, antecedent and consequence. No prior literature has examined the impact of top management’s GTL on GOLC and CA.
Nearby large islands diminish biodiversity of the focal island by a negative target effect
Authors: Wang Duorun , Zhao Yuhao , Tang Shupei , Liu Xiangxu , Li Wande , Han Peng , Zeng Di , Yang Yangheshan , Wei Guangpeng , Kang Yi , Si Xingfeng 
Published: December 2022

Abstract

The Equilibrium Theory of Island Biogeography postulates that larger and closer islands support higher biodiversity through the dynamic balance of colonization and extinction processes. The negative diversity–isolation (i.e. the distance to the mainland) relationship is derived based on the assumption that the mainland is the only source pool for island biotas. However, nearby islands could also act as species sources for focal islands via a source effect. In this study, we move a further step and hypothesize that nearby islands may reduce bird colonizers of the focal island and diminish its biodiversity, resulting in a negative target effect.

To test our hypothesis, we assessed the effects of island area and isolation (metrics considering both the mainland and nearby islands) on taxonomic (i.e. species richness), functional and phylogenetic diversity of terrestrial breeding birds on 42 islands in the largest archipelago of China, the Zhoushan Archipelago. Furthermore, we compared the predictive power of the distance to the large island under a set of relative area thresholds and the relative area of nearby islands on species richness under a set of distance thresholds to explore the role of nearby islands as a source and/or target island.

We found that island area had a positive effect on species richness, phylogenetic diversity and functional diversity, while the distance to the mainland had a negative effect only on species richness. Species richness on the focal island increased with increasing distance to the nearest larger island, indicating the negative target effect. Furthermore, the negative target effect depended on the area of nearby islands relative to the area of the focal island.

Our finding of the negative target effect suggests islands located between the mainland and the focal island can be not only sources or stepping stones, but also colonization targets. This result demonstrates the importance of considering multiple geographical attributes of islands in island biogeographic studies, especially the characteristics related to source and/or target effects.