bluuebunny/crossref_metadata_2025_split huggingface dataset:
International Spinal Cord Injury Core Data Set (version 3.0)—including standardization of reporting
Authors: Biering-Sørensen Fin , Charliefue Susie , Chen Yu-ying , New Peter , Noonan Vanessa , Post Marcel , Rupp Ruediger , Vogel Lawrence 
Published: August 2022

Abstract
Study design: Expert opinion, feedback, revisions and final consensus.Objectives: To update the International Spinal Cord Injury (SCI) Core Data Set while still retaining recommended standardization of data reporting.Setting: International.Methods: Comments on the data elements received from the SCI community were discussed in the International Core Data Set working group. The suggestions from this group were iteratively reviewed. The final version was circulated for final approval.Results: The International SCI Core Data Set (Version 3.0) consists of 21 variables. The variable ‘Gender’ has been changed to ‘Sex assigned at birth’; for the variable ‘Spinal Cord Injury Etiology’, the item, ‘Sports or exercise during leisure time’, has been clarified as ‘including during leisure time’; for the variables ‘Vertebral injury’ and ‘Associated injuries’, the item ‘Unknown’ is reworded into: ‘Not applicable (non-traumatic case)’ and ‘Unknown’; the variable ‘Spinal surgery’ has been expanded to include surgeries for individuals with non-traumatic SCI; for the variables related to the International Standards for Neurological Classification of SCI only the neurological level of injury (NLI) and the American Spinal Injury Association (ASIA) Impairment Scale (AIS) are to be reported, and a separate variable is included indicating if the NLI or the AIS or both are impacted by a non-SCI condition. Conclusion: The International SCI Core Data Set (Version 3.0) should be collected and reported for all studies of SCI to facilitate uniform descriptions of SCI populations and facilitate comparison of results collected worldwide.
Ginger essential oil prevents NASH progression by blockading the NLRP3 inflammasome and remodeling the gut microbiota-LPS-TLR4 pathway
Authors: Sheen Lee-Yan , Panyod Suraphan , Wu Wei-Kai , Hsieh Ya-Chi , Tseng Yea-Jing , Peng Sin-Yi , Chen Rou-An , Huang Huai-Syuan , Chen Yi-Hsun , Shen Ting-Chin David , Ho Chi-Tang , Liu Chun-Jen , Chuang Hsiao-Li , Huang Chi-Chang , Wu Ming-Shiang 
Published: August 2022

Abstract
Diet and gut microbiota contribute to non-alcoholic steatohepatitis (NASH) progression. High-fat diets (HFDs) change gut microbiota compositions and induce gut dysbiosis and intestinal barrier leakage, which facilitates portal influx of pathogen-associated molecular patterns [e.g., lipopolysaccharides (LPS)] to the liver and triggers inflammation in NASH. The current therapeutic drugs for NASH have adverse side effects; however, several foods and herbs have exhibited hepatoprotection. We investigated ginger essential oil (GEO) against palm oil-containing HFDs in LPS-injected murine NASH model. GEO prevented NASH development by increasing plasma alanine aminotransferase levels; increasing antioxidant catalase, glutathione reductase, and glutathione levels; and reducing hepatic pro-inflammatory cytokine levels. GEO alleviated hepatic inflammation through mediated NLR family pyrin domain-containing 3 inflammasome and LPS/Toll-like receptor 4 signaling pathways. GEO further increased beneficial bacterial abundance and reduced NASH-associated bacterial abundance. GEO may, therefore, prevent NASH by inhibiting hepatic inflammation and modulating the gut microbiota and its metabolite-related pathway.
Change in Head Impact Exposure following Return to Play in Concussed Football Athletes
Authors: Muench Jared R. , Sinson Grant P. , Harezlak Jaroslaw , Rowson Steven , Duma Stefan , Brooks Alison , Cameron Kenneth , Giza Christopher , Goldman Joshua , McGinty Gerald , Riggen Larry Dwight , Pasquina Paul F. , Broglio Steven P. , McAllister Thomas , McCrea Michael A. , Stemper Brian D. 
Published: August 2022

Abstract
Background
Following sport-related concussion, early head impact exposure and premature return to play is known to increase risk of repeat concussion in football athletes, yet athletes’ true post-injury head impact exposure profiles and biomechanical progression have not been explored. Accordingly, this study explored how American college football athletes alter playing style during their return from concussion, particularly within the same athletic season.
Methods
Using head impact exposure profiles of fifty-two collegiate football athletes from six NCAA Division I programs, this analysis compared daily volume of head impacts following concussion with pre-injury levels and further compared these athletes to team- and position-matched controls to minimize season- or team-related factors. Additionally, this study provided an analysis of the possible association between length of recovery time and change in head impact exposure following concussion using continuous linear regression.
Results
Over 40% of concussed athletes did not reach their pre-injury level of head impact exposure at any point during the remainder of the season segment that the concussion occurred. Furthermore, concussed athletes showed significantly larger decreases than non-concussed athletes over the same time period. Finally, longer recovery times after concussion were associated with larger decreases in head impact exposure over the remaining contact days within the season segment.
Conclusions
This study provide evidence for a shift in playing style after returning from concussion, both in the immediate days after return to play as well as over extended periods of time within the season. These findings will help to better inform the management of athletes returning from concussion.
Can a three months treatment with oral Desogestrel prior to insertion of the etonogestrel subdermal implant improve continuation rate at one year? A randomized trial
Authors: Fäh Valeria Lombardi , Catarino Rosa , Castillo Sarah , Badda Maria , Gezer-Dickschat Sibel , Thieringer Friederike , Tschudin Sibil , Viviano Manuela , Yaron Michal 
Published: August 2022

Abstract
Objective: The aim of our study is to evaluate if daily oral 75µg of Desogestrel (DSG) for 3 months prior to the insertion of etonogestrel subdermal implant (ENG-IMPLANT) might help reduce its premature discontinuation.Results: A total of 66 women were randomized in the ENG-IMPLANT group (26) and in the DSG+ENG-IMPLANT group (40), respectively, in the Geneva University Hospitals and Basel University Hospital, from August 15th, 2016 through September 30th, 2019. In the DSG+ENG-IMPLANT group, patients were given a 3 months’ supply of 75µg of DSG before the insertion of the ENG-IMPLANT. All women were seen after 3 months for bleeding and satisfaction evaluation, and at 12 months post ENG-IMPLANT insertion. Higher levels of satisfaction at 12-months were found in the ENG-IMPLANT group compared to the DSG+ENG-IMPLANT group (8.5±1.7 vs. 6.6±2.9, p=0.012). There were no statistically significant differences regarding tolerance (7.8±2.5 vs 6.8±2.6, p=0.191) and contraceptive continuation (80% vs 72.4%, p=0.544) between groups. DSG prior to insertion of the ENG-IMPLANT did not improve its continuation rate neither its satisfaction at one year.Trial registration: NCT05174195. Retrospectively registered, the 30th December 2021.
Patient and providers-level drivers of healthcare utilization related to a diagnosis of a precancerous condition: monoclonal gammopathy of undetermined significance (MGUS)
Authors: Castaneda-Avila Maira A. , Mazor Kathleen M. , Lapane Kate L. , Epstein Mara M. 
Published: August 2022

Abstract
Purpose
Monoclonal gammopathy of undetermined significance (MGUS) is the precursor of multiple myeloma. This qualitative study described patient (n = 14) experiences and healthcare providers’ (n = 8) opinions and practices concerning care for patients with MGUS in the US.
Methods
Semi-structured, in-depth interviews were analyzed using thematic analysis.
Results
We identified six overarching themes related to the care pathway for patients with MGUS. (1) Process of MGUS diagnosis, (2) Providers’ explanations, (3) Patients’ understanding, (4) Impact of the diagnosis, (6) follow-up/management, and (6) factors influencing healthcare utilization. Patients demonstrated a basic understanding of MGUS. However, some patients felt anxiety around the diagnosis, which may affect other aspects of their lives. Non-hematologist providers report having less MGUS-specific knowledge. Older age, high-risk MGUS, and insurance/cost influenced healthcare utilization.
Conclusion
Patients with MGUS may have difficulty processing this premalignant diagnosis. Non-hematologist providers may have gaps in knowledge around specific care for patients with MGUS.
oFlowSeq: A multiplexed quantitative approach to identify genes affecting cell type enrichment using mosaic CRISPR-Cas9 edited cerebral organoids
Authors: Dawes Pepper , Smullen Molly , Fernandez-Fontaine Aria , Zhang Yucheng , English Jay , Uddin Mohammed , Pak ChangHui , Church George M , Chan Yingleong , Lim Elaine T 
Published: August 2022

Abstract
Cerebral organoids are comprised of diverse cell types found in the developing human brain, and can be leveraged for the identification of critical cell types perturbed by genetic risk variants in common, neuropsychiatric disorders. There is great interest in developing high-throughput, multiplexed technologies to associate genetic variants with cell types. Here, we describe a multiplexed, quantitative approach (oFlowSeq) by utilizing CRISPR-Cas9, FACS sorting and next-generation sequencing. Using oFlowSeq, we found that deleterious mutations in autism-associated gene KCTD13 resulted in increased proportions of Nestin+ cells and decreased proportions of TRA-1- 60+ cells within mosaic cerebral organoids. We further identified that a locus-wide CRISPR-Cas9 survey of another 19 genes in the 16p11.2 locus resulted in >2.5% maximum editing efficiencies for short and long indels, suggesting a high feasibility for an unbiased, locus-wide experiment using oFlowSeq. Our approach presents a novel method to identify genotype-to-cell type imbalances in an unbiased, multiplexed, quantitative manner.
500 Wh/kg Class High-Areal-Capacity Lithium Metal Pouch Cell Enabled by Practical Polymer Protective Layer
Authors: Feng Yangyang , Li Yong , Lin Jing , Zhu Lei , Zhang Xiang , Zhang Linlin , Sun Chuan-Fu , Wu Maoxiang , Wang Yaobing 
Published: August 2022

Abstract
Stable high-areal-capacity lithium metal under practical conditions is decisive to reach 500 Wh/kg, which is plagued by undesirable lithium-electrolyte interface. Herein, we design and synthesize poly(2-hydroxyethyl acrylate-co-sodium benzenesulfonate) (PHS) as lithium-electrolyte interfacial protective layer, containing soft poly (2-hydroxyethyl acrylate) (PHEA) with hydroxyl to provide strong interaction with Cu substrate by hydrogen-bond and improve the flexibility, and ion-conductive poly(sodium p-styrene sulfonate) (PS) with large Li chemisorption energy to anchor and disperse Li+. Cryo-TEM images revel that multifunctional PHS induces the formation of rigid and crystalline fluorinated SEI inner layers. The theoretical calculations confirm that -SO3− in PS promotes Li+ towards interchain migration through cation-dipole interaction, enabling uniform Li+ diffusion, achieving average Coulombic efficiency of 99.46% under 6 mAh/cm2 for Li|PHS-Cu cell. When paired with high-areal-capacity NCM83 (6.0 mAh/cm2), the assembled NCM83|PHS-Li pouch cell with the capacity of 6.87 Ah can reach 490.4 Wh/kg and exhibits 92.9% capacity retention after 150 cycles.
Phylogeny and adaptive evolution of subgenus Rhizirideum (Amaryllidaceae, Allium) based on plastid genomes
Authors: Fu Xiao , Xie Deng-Feng , Zhou Yu-Yang , Cheng Rui-Yu , Zhang Xiang-Yi , Zhou Song-dong , He Xing-Jin 
Published: August 2022

Abstract
The subgenus Rhizirideum in genus Allium consists of 38 species across the world. Previous studies on this subgenus mainly focused on separate sections. To investigate the adaptive evolution and phylogenetic relationships of this subgenus, we selected eleven species of subgen. Rhiziridem to conduct the comparative plastome analysis. As a result, the Rhizirideum plastomes were relatively conservative in terms of structure, IR/SC borders, codon usage, and repeat sequence. Unlike the previous reports, A. polyrhizum, representing section Caespitosoprason, was resolved as the basal taxon of subgen. Rhizirideum. Most of the single-copy CDSs were under purifying selection, while eleven genes presented relaxed selection, such as atpB, psbD and rbcL, which were mainly involved in the photosynthesis and respiration processes. The pseudogenization and relaxed selection of genes, the introns, the slight shift of IR/SC borders and the low GC content can be ragarded as the adaptation of plants to the environment. Our study highlighted the advantage of plastid genomes in reconstructing phylogenetic relationships. Also, we considered that section Caespitosoprason needed to be separated from section Rhizomatosa. In summary, our research provided new insights into the phylogeny, adaptive evolution, and taxonomy of subgen. Rhizirideum.
Altered intestinal flora in patients with primary immune thrombocytopenia
Authors: Wang Honghui , Bi Hui , Yang Muran , Wang Xiuli , Song Chuanju , Yang Wen , Wang Yacan , Xie Dongmei , Li Huiting , Zhou Zeping 
Published: August 2022

Abstract
BackgroundLittle is known about the intestinal flora of primary immune thrombocytopenia (ITP) patients in relation to those of healthy people. AimTo explore the structural and functional differences of intestinal flora between ITP patients and healthy controls.MethodsFeces from ITP patients and healthy controls were studied by 16S rRNA and metagenomic techniques at phylum, genus, species or functional levels. Blood samples were collected for the detection of IL-2 and IL-4 concentrations.ResultThe following changes in ITP patients were found: a decrease of Bacteroidetes phylum, an increase of Proteobacteria phylum and alterations of ten genera and 1045 species. Genera of Phascolarctobacterium and Romboutsia were correlated with IL-2 levels. Species of C. freundii, C. rodentium, and C. youngae were negatively correlated with bleeding scores, and S. infantis was positively related to platelet counts. Functionally, the intestinal flora of ITP patients changed mainly in terms of motility, chemotaxis, membrane transport, and metabolism. ConclusionsThe mechanism underlying the functional and structural changes of the intestinal flora in ITP patients may be related to inflammation and immunity, suggesting a new target for ITP treatment.
An Overview of Cervical Cancer Epidemiology and Prevention in the Baltic States
Authors: Kojalo Una , Tisler Anna , Parna Kersti , Kivite-Urtane Anda , Zodzika Jana , Stakunas Mindaugas , Baltzer Nicholas , Nygard Mari , Uuskula Anneli 
Published: August 2022

Abstract
AimsTo inform future Baltic States-specific policy analyses, we aimed to provide an overview of cervical cancer epidemiology and existing prevention efforts in Estonia, Latvia and Lithuania.MethodsA structured desk review: we compiled and summarized data on current prevention strategies, population demography and epidemiology (high risk human papillomavirus (HPV) prevalence and cervical cancer incidence and mortality over time) for each Baltic State by reviewing published literature and official guidelines, performing registry-based analyses using secondary data and having discussions with experts in each country. ResultsWe observed important similarities in the three Baltic States: high burden of the disease (high incidence and mortality of cervical cancer, changes in TNM (Classification of Malignant Tumors) stage distribution towards later stage at diagnosis), high burden of high-risk HPV in general population and suboptimal implementation of the preventive strategies as low screening and HPV vaccination coverage.ConclusionsCervical cancer remains a substantial health problem in the region and the efforts in addressing barriers by implementing a four-step plan for elimination cervical cancer in Europe should be made. This goal is achievable through evidence-based steps in four key areas: vaccination, screening, treatment, and public awareness.
An examination of sleep spindle metrics in the Sleep Heart Health Study: superiority of automated spindle detection over total sigma power in assessing spindle activity
Authors: Palepu Kalyan , Sadeghi Kolia , Kleinschmidt Dave , Donoghue Jacob , Chapman Seth , Arslan Alex , Westover M. Brandon , Cash Sydney S. , Pathmanathan Jay 
Published: August 2022

Abstract
Background. Sleep spindle activity is commonly estimated by measuring sigma power during stage 2 non-rapid eye movement (NREM2) sleep. However, spindles account for little of the total NREM2 interval and therefore sigma power over the entire interval may be misleading. This study compares derived spindle measures from direct automated spindle detection with those from gross power spectral analyses for the purposes of clinical trial design.Methods. We estimated spindle activity in a set of 8,440 overnight electroencephalogram (EEG) recordings from 5,793 patients from the Sleep Heart Health Study using both sigma power and direct automated spindle detection. Performance of the two methods was evaluated by ability to distinguish NREM2 from other NREM sleep stages and by determining the sample size required to detect decline in age-related spindle coherence with each method in a simulated clinical trial. Results. Mean sigma power in NREM2 was indistinguishable from other NREM states in 25% of recordings, vs only 7% for direct spindle measurement (P < 0.001). These percentages were 34% and 14% respectively for sigma coherence comparisons. In a simulated clinical trial, sigma power required a sample size of 115 to achieve 95% power to identify age-related changes in sigma coherence, while automated spindle detection required a sample size of only 60.Conclusions. Measurements of spindle activity utilizing automated spindle detection outperformed conventional sigma power analysis by a wide margin, suggesting that many studies would benefit from incorporation of automated spindle detection. These results further suggest that some previous studies which have failed to detect changes in sigma power or coherence may have failed simply because they were underpowered.
S-nitrosylation of CSF1 receptor increases the efficacy of CSF1R blockage against Prostate cancer
Authors: Kuchakulla Manish , Firdaus Fakiha , QURESHI REHANA , Soni Yash , Booven Derek , SHAH KHUSHI , Dulce Raul Ariel , Masterson Thomas , Rosete Omar , Punnen Sanoj , Hare Joshua , RAMASAMY RANJITH , ARORA HIMANSHU 
Published: August 2022

Abstract
Sustained oxidative overload in castration-resistant prostate cancer (CRPC) cells potentiates the overall tumor microenvironment (TME). Targeting the TME using colony-stimulating factor 1 receptor (CSF1R) inhibition is a promising therapy for CRPC. However, the therapeutic response to sustained CSF1R blockade therapy (CSF1Ri) is limited as a monotherapy. We postulated that one of the causative agents for reduced efficacy of CSF1Ri and increased oxidation in CRPC is endothelial nitric oxide syntheses (eNOS). Results showed that in high grade PCa human specimens, eNOS is positively correlated with CSF1-CSF1R signaling and remains in an un-coupled state. The uncoupling disables eNOS to generate sufficient Nitric oxide (NO) that are required for inducing effective S-nitrosylation of CSF1R molecule at specific cysteine sites (Cys 224, Cys 278 and Cys 830). We found that exogenous NO treatment (using S-nitrosoglutathione (GSNO)) is effective in not only inducing S-Nitrosylation of CSF1R molecule, but it helps in rescuing the excess oxidation in tumor regions, suppresses the tumor promoting cytokines which are ineffectively suppressed by CSF1R blockade. Together these results postulated that NO therapy could act as an effective combinatorial partner with CSF1R blockade against CRPC. In this context, results demonstrated that exogenous NO treatment successfully augment the anti-tumor ability of CSF1Ri to effectively reduce the overall tumor burden, decrease the intratumoral percentage of anti-inflammatory macrophages, myeloid derived progenitor cells and increasing the percentage of pro-inflammatory macrophages, cytotoxic T lymphocytes, and effector T cells respectively. Together, our study suggests that the NO-CSF1Ri combination has the potential to act as a therapeutic agent that restore control over TME and improve the outcomes of PCa patients.
Experimental Realization of a High-Finesse Laser Cavity based on a Low-Noise Microstructured Mirror
Authors: Dickmann Johannes , Sauer Steffen , Meyer Jan , Gaedtke Mika , Siefke Thomas , Brückner Uwe , Plentz Jonathan , Kroker Stefanie 
Published: August 2022

Abstract
The most precise measurement tools of humankind are equipped with ultra-stable lasers. State-of-the-art laser stabilization techniques are based on external cavities, that are limited by noise originated in the coatings of the cavity mirrors. Microstructured mirror coatings (so-called meta-mirrors) are a promising technology to overcome the limitations of coating noise and therewith pave the way towards next-generation ultra-stable lasers. We present the first experimental realization of a 10,000-finesse optical cavity based on a low-noise meta-mirror, representing a milestone in this field. The symbiosis of the presented mirror technology with silicon cavity spacers will result in a 5×10−18 laser stability, which is an order of magnitude improvement.
Role of long noncoding RNAs; BDNF-AS and 17A and their relation to GABAergic dysfunction in Egyptian epileptic patients
Authors: Zayed Aya A. , Seleem Mae M. , Darwish Hebatallah , Shaheen Amira A. 
Published: August 2022

Abstract
Epilepsy is a chronic neurological disorder characterized by recurrent unprovoked seizures. Lately, long noncoding RNAs (lncRNAs) have been increasingly appreciated as regulators of epilepsy-related processes, however, their functional role in its pathogenesis is still to be explored. This study investigated the expression levels of lncRNAs; BDNF-AS and 17A in the sera of Egyptian patients with idiopathic generalized and symptomatic focal epilepsy and correlated their levels with brain-derived neurotrophic factor (BDNF), phosphorylated cAMP reaction element -binding protein (p-CREB), gamma- aminobutyric acid (GABA) and glutamate, to underline their related molecular mechanism. A total of 70 epileptic patients were divided into two clinical types, besides 30 healthy controls of matched age and sex. The expression levels of both lncRNAs were markedly upregulated in epileptic groups versus healthy control with predominance in the symptomatic focal one. Epileptic patients showed significantly lower levels of BDNF, p-CREB, GABA along with significant increases of glutamate levels and glutamate/ GABA ratio, especially in symptomatic focal versus idiopathic generalized epileptic ones. The obtained data raised the possibility that these lncRNAs might be involved in the pathogenesis of epilepsy via inhibition of GABA/p-CREB/BDNF pathway. The study shed light on the putative role of these lncRNAs in better diagnosis of epilepsy, particularly symptomatic focal epilepsy.