bluuebunny/crossref_metadata_2025_split huggingface dataset:
Usefulness of simulation in optimizing imaging settings for pediatric technetium-99m-dimercaptosuccinic acid single-photon emission computed tomography
Authors: Kurosawa Yuji , Kanzaki Takao , Higuchi Tetsuya , Suto Takayuki , Tsushima Yoshito 
Published: June 2022

Purpose
Technetium-99m (99mTc) dimercaptosuccinic acid (DMSA) single-photon emission computed tomography (SPECT) has been used to diagnose renal scarring. The Japanese Society of Nuclear Medicine recently revised the ‘Consensus Guidelines for Pediatric Nuclear Medicine Examination.’ In this study, we compared simulation data with actual data obtained using a pediatric phantom for 99mTc-DMSA examinations and evaluated the usefulness of simulations in determining the optimal acquisition conditions for SPECT images.

Methods
A SPECT quality assurance (QA) phantom study produced images with a renal-to-background 99mTc ratio of 283:1 kBq/ml. The projection data for the simulation were simulated using the simulation of imaging nuclear detectors. To compare the actual measurements and simulations, recovery factors were used for the SPECT QA phantom for image quality assessment. Defect contrast and visual evaluation using Scheffe’s method of pairwise comparison were used for the pediatric kidney phantom.

Results
The optimal imaging settings using a kidney phantom required an acquisition time of more than 8 min. The maximum difference in the recovery coefficient between the simulation and actual measurement using the SPECT QA phantom was 6%.

Conclusion
We showed that an acquisition time of more than 8 min was necessary for DMSA-SPECT. In addition, phantom simulations were approximately equivalent to the actual measurement data and the adaptability of simulations was confirmed.
Hepatic superscan on fluorine-18 fluorodeoxyglucose PET/computed tomography imaging: a specific manifestation for diagnosing lymphoma or leukemia involvement
Authors: Wang Lijuan , Jiang Yanping , Zhang Yin , Zhou Wenlan , Li Hongsheng , Wu Hubing 
Published: June 2022

Objective
To explore whether the markedly fluorine-18 fluorodeoxyglucose (18F-FDG) uptake in the liver (named hepatic superscan) is a specific manifestation of malignant involvement.

Methods
From January 2014 to June 2019, 23 patients with such presentations were retrospectively reviewed. 18F-FDG uptake was semiquantified using maximal standardized uptake value (SUVmax), liver to cerebellum (L/C) ratio, liver to mediastinum (L/M) ratio, mean standardized uptake value (SUVmean), peak standardized uptake value (SUVpeak), metabolic tumor volume (MTV) and total lesion glycolysis (TLG). Some related laboratory examinations were also collected and analyzed. For comparison, 37 patients with mildly and moderately uptake in the liver were selected as the control group.

Results
The hepatic SUVmax, L/C ratio, L/M ratio, SUVmean, MTV and TLG of the superscan group were significantly higher than that of mild- or moderate-uptake group (P < 0.005). Malignant hematological tumors accounted for 91.3% of the superscan group, which was significantly higher than 51.4% of mild- or moderate-uptake group (P = 0.004). β2-microglobulin was observed to be significantly higher in the superscan group compared with mild- or moderate-uptake group (P < 0.001), but not lactate dehydrogenase (LDH) (P = 0.409). On the contrary, C-reactive protein (CRP) was significantly higher in mild- or moderate-uptake group than that in the superscan group (P < 0.001).

Conclusion
Our study demonstrates that hepatic superscan is a strong indicator of malignant hematological tumors invading the liver.
Decoding germ cell tumours for clinicians
Authors: Haider Aiman , Verrill Clare , Alifrangis Constantine , Berney Daniel Maurice 
Published: June 2022

Purpose of review
Germ-cell tumours of the testis affect predominantly younger males aged between 15 and 40 years, with nearly 74,500 new cases estimated globally in 2020. Their rarity and the complex morphology, mean that, in nonexpert hands, there is a significant risk of misdiagnosis of both type and staging of these neoplasms.

Recent findings
There have been significant changes in the 2016 WHO classification of Testicular tumours that need to be understood by both pathologists and clinicians for streamlining management. Standardised structured reporting guidelines and discussion at the multidisciplinary-team meetings lead to subsequently better health outcomes and patient safety.

Summary
Therefore, communication with high-quality reports and understanding of clinicians of what constitutes an adequate report, is the key to ensure proper management of these patients. We attempt to discuss the key updates and pathological features that influence management and need to be communicated with clarity and precision.
Sex cord stromal tumors and tumors of the paratestis: new and old entities in a landscape of rare tumors
Authors: Colecchia Maurizio , Raspollini Maria Rosaria , Bremmer Felix , Pini Giacomo Maria 
Published: June 2022

Purpose of review
The 5th edition of WHO classification incorporates the most relevant new data available in the literature regarding tumors of the male genitourinary tract. In this review, the authors summarize and critically discuss the most relevant new information regarding tumors occurring in the stromal testis and in the paratestis that will be reported in the new edition of WHO classification of tumors of the male genitourinary tract.

Recent findings
Signet-ring stromal tumors (SRST) and myoid gonadal stromal tumors (MGST) are two new entities brought in the 5th WHO classification of testicular tumors. All cases of SRST and MGST reported so far have behaved in a benign fashion after resection and whenever possible a conservative surgery is recommended. A future perspective is to aim at creating large multiinstitutional case series to link different morphologic patterns and molecular bases to the biologic behavior of these neoplasms. Another innovation in WHO consists in the inclusion in the group of Sertoli cell tumors of the sertoliform cystadenoma. The sertoliform cystadenoma is localized in the rete testis and it is of unknown origin. It was included in the group of gonadal stromal tumors because of a high morphological and immunohistochemical similarity to the Sertoli cell tumor.

Summary
Although further studies with long-term follow-up are needed to estimate the main oncologic outcomes in patients with rare gonadal stromal tumors, we highlight the importance of an accurate characterization by molecular and immunohistochemical assays of these entities.
Electromagnetic-guided puncture: a tool or a tale?
Authors: Tsaturyan Arman , Liatsikos Evangelos , Faitatziadis Solon , Kallidonis Panagiotis 
Published: June 2022

Purpose of review
The puncture technique and site of the puncture can impact the clinical outcome and the development of postoperative adverse events following percutaneous nephrolithotomy (PCNL). The aim of the current review was to discuss the available puncture guidance approaches and outline the potential role of the recently introduced electromagnetic-guided method.

Recent findings
The puncture and PCNL tract establishment is usually performed using fluoroscopic or ultrasound guidance or a combination of two approaches. Electromagnetic-guided puncture is one of the most recent advancements of technology. The puncture navigation to the renal collecting system is available after placing a special wire with an electromagnetic tracking sensor into the desired calyx through the flexible ureterorenoscope. The available experimental and clinical studies have shown a high first puncture rate, decreased median time for obtaining a puncture, and shorter learning curves for beginners.

Summary
Fluoroscopic and ultrasound guidance are the main approaches used by urologists. However, these modalities require a steep learning curve, approximately 60 cases for reaching competency. The initial data on electromagnetic navigation have shown the feasibility and potential promising outcomes of this novel approach. New studies are required to investigate its potential implementation opportunities in the daily practice of urologists.
Maternal Distress in Disclosing a Nonlethal Fetal Anomaly Diagnosis to Family and Friends
Authors: Tucker Janet Adams , Christian Becky J. 
Published: June 2022

Abstract

Purpose:
To describe experiences of women disclosing a nonlethal fetal anomaly diagnosis to family and friends from initial prenatal diagnosis through postpartum.

Study Design and Methods:
A descriptive, qualitative approach was used to explore women's perceptions of nonlethal fetal anomaly diagnosis in a high-risk obstetric clinic. In-depth interviews were conducted with 10 pregnant women, followed by postpartum interviews with 8 women. Data were analyzed using thematic analysis.

Results:
Analysis of semistructured interviews revealed an overarching theme, Pregnancy Forever Changed, which captured the experience when expectant women first heard the news about their fetus. Prenatal themes were News of a Diagnosis, No Going Back; A Mother's Response: Managing Information; and Words from Others Matter. Women's struggles continued into postpartum, The Journey Continues: Echoing Past Concerns; and Not the Journey We Planned.

Clinical Implications:
Women with a nonlethal fetal anomaly diagnosis experienced distress and stigma about disclosure of the diagnosis to others. Distress with disclosure continued throughout the pregnancy and extended into postpartum. Women described negative responses from others and ongoing management of information as stressful and painful. Clinicians are aware disclosing a lethal diagnosis is distressing for women but may not understand the experience of women with a nonlethal diagnosis. An appreciation of women's distress on disclosure of nonlethal fetal anomaly diagnoses can guide practice for maternity, neonatal, and pediatric nurses providing interventions that include information and anticipatory guidance.
Infant Safe Sleep Initiative in a Small Volume Maternity Service
Authors: Patterson Kimberly J. , Adams Ellise D. , Ramieh Charlotte 
Published: June 2022

Abstract

Purpose:
Despite the 2016 American Academy of Pediatrics recommendations for preventing sudden unexpected infant death, there are approximately 3,400 sudden unexpected infant deaths annually in the United States. Modeling of safe sleep practices by nurses in the hospital influences parents' practice at home; however, safe sleep practices are not consistently modeled during the childbirth hospitalization.

Methods:
A quality improvement project was conducted to implement a safe sleep initiative based on the 2016 American Academy of Pediatrics recommendations for nurses caring for hospitalized infants after birth. Evaluation included testing of nurses' knowledge, infant crib audits, and nurses' evaluation of the intervention.

Results:
A significant improvement was found in overall nurse education scores. Crib audits demonstrated a significant improvement in the following elements: use of multiple blankets, swaddling of the infant, and parent teaching. Nursing surveys reported an increase in confidence to practice safe sleep recommendations and educate and redirect parents.

Clinical Implications:
Implementing a safe sleep initiative can increase nurses' knowledge, improve adherence to recommendations with modeling safe sleep practices, and increase parent awareness of safe sleep recommendations, potentially positively affecting adherence after discharge.
Thoracic spine deformation may predict prosthetic rehabilitation outcome
Authors: Kim Mindae 
Published: June 2022

Background:
A considerable number of patients require prosthetic ambulation, which necessitates improvements in the success rate of prosthetics.

Objective:
This study aimed to determine the association between the presence of thoracic spinal osteophyte on imaging and the patient’s ability to walk with lower leg prosthetics.

Study design:
Retrospective cohort study.

Methods:
This study included 15 patients with lower leg amputations who underwent prosthetic rehabilitation. Medical records and spine radiographs of patients with lower leg amputations between 2013 and 2019 at a private rehabilitation hospital were reviewed. The primary outcome was achieving prosthetic ambulation with a single T-cane or without a walking aid.

Results:
Poor outcomes were associated with the presence of thoracic spinal deformation (P = 0.007) and the lower Berg Balance Scale score at admission (P = .067) and discharge (P = .033) groups. Monte Carlo simulation results showed a ≥33% difference in walking achieving rate between patients with and without thoracic spinal deformity. In addition, an 18% probability was found for randomly selected persons with an amputation to show a difference of ≥1 point in Berg Balance Scale scores.

Conclusion:
Thoracic spinal deformity on radiography and Berg Balance Scale score at admission are potentially associated with successful prosthetic rehabilitation. These findings potentially affect the indications for prosthetic limbs.
Extracellular Vesicles Released During Normothermic Machine Perfusion Are Associated With Human Donor Kidney Characteristics
Authors: Woud Wouter W. , Arykbaeva Asel S. , Alwayn Ian P.J. , Baan Carla C. , Minnee Robert C. , Hoogduijn Martin J. , Boer Karin 
Published: June 2022

Background.
Extracellular vesicles (EVs) are tissue-specific particles released by cells containing valuable diagnostic information in the form of various biomolecules. The characterization of EVs released by kidney grafts during normothermic machine perfusion (NMP) may present a promising avenue to assess graft status before transplantation.

Methods.
We phenotyped and determined the concentrations of EVs in the perfusate of 8 discarded expanded-criteria donor human kidneys during 6 h of NMP. Perfusate samples were taken at 0/60/180/360 min and examined with nanoparticle tracking analysis and imaging flow cytometry (IFCM). Using IFCM, EVs were identified by their expression of common EV markers CD9, CD63, and CD81 (tetraspanins) in combination with either platelet endothelial cell adhesion molecule (CD31), pan-leukocyte protein (CD45), or carboxyfluorescein succiminidyl ester (CFSE) fluorescence.

Results.
Nanoparticle tracking analysis measurements revealed the release of nanoparticles <400 nm into the perfusate during NMP. With IFCM, tetraspanin protein signatures of the released nanoparticles were characterized, and the majority (~75%) of CFSE+ EVs were found to be CD81+, whereas ~16% were CD9+ and ~8% CD63+. Correlation analysis of concentrations of identified EV subsets with crude donor characteristics and NMP viability characteristics revealed significant correlations with cold ischemia time, donor age, and renal flow.

Conclusions.
Our findings demonstrate that discarded expanded-criteria donor kidney grafts release distinct EV subsets during NMP. Because these subsets correlate with well-established indicators of transplant outcome, EVs might represent new potential candidates for assessment of kidney graft quality.
Cancer related subarachnoid hemorrhage: a multicenter retrospective study using propensity score matching analysis
Authors: K Senthil 
Published: June 2022

ABSTRACTObjectiveTo investigate the clinical features, risk factors and underlying pathogenesis of cancer related subarachnoid hemorrhage (SAH).MethodsClinical data of SAH in patients with active cancer from January 2010 to December 2020 at four centers were retrospectively reviewed. Patients with active cancer without SAH were matched to SAH patients with active cancer group. Logistic regression was applied to investigate the independent risk factors of SAH in patients with active cancer, after a 1:1 propensity score matching. A receiver operator characteristic curve was configured to calculate the optimal cut-off value of the joint predictive factor for cancer related SAH.ResultsA total of 82 SAH patients with active cancer and 309 patients with active cancer alone were included. Most SAH patients with cancer had poor outcomes, with thirty-day mortality of 41.5%, and with ninety-day mortality of 52.0%. The propensity score matching yielded 75 pairs of study participants. Logistic regression revealed that a decrease in platelet and prolonged prothrombin time were the independent risk factors of cancer related SAH. In addition, receiver operator characteristic curve of the joint predictive factor showed the largest AUC of 0.8131, with cut-off value equaling to 11.719, with a sensitivity of 65.3% and specificity of 89.3%.ConclusionsPatients with cancer related SAH often have poor outcomes. The decrease in platelet and prolonged prothrombin time are the independent risk factors of cancer related SAH, and the joint predictive factor with cutoff value equal to 11.719 should hence serve as a novel biomarker of cancer related SAH.
HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancer
Authors: Foster John G , Gea Esteban , Labiba Mosammat A , Anene Chinedu A , Stockley Jacqui , Philippe Celine , Cereda Matteo , Rouault-Pierre Kevin , Leung Hing , Bessant Conrad , Rajan Prabhakar 
Published: June 2022

AbstractHNRNPA2B1 is associated with prostate cancer (PC) disease aggressiveness and underlies pro-tumourigenic cellular stress responses. By analysing >500 PC transcriptomes, we reveal that HNRNPA2B1 over-expression is associated with poor patient prognosis and stress response pathways. These include the “protein processing in the endoplasmic reticulum” (ER) pathway, which incorporates the unfolded protein response (UPR). By RNA-sequencing of HNRNPA2B1-depleted cells PC cells, we identified HNRNPA2B1-mediated down-regulation of UPR genes including the master ER-stress sensor IRE1, which induces ER proteostasis. Consistent with IRE1 down-regulation in HNRNPA2B1-depleted cells, we observed reduced splicing of the IRE1-target and key UPR effector XBP1s. Furthermore, HNRNPA2B1 depletion up-regulates expression of the IRE1-dependent decay (RIDD) target gene BLOC1S1, which is degraded by activated IRE1. We identify a HNRNPA2B1-IRE1-XBP1-controlled four gene prognostic biomarker signature (HIX) which classifies a subgroup of primary PC patients at high risk of disease relapse. Pharmacological targeting of IRE1 attenuated HNRNAPA2-driven PC cell growth. Taken together, our data reveal a putative novel mechanism of UPR activation in PC by HNRNPA2B1, which may promote an IRE1-dependent yet potentially-targetable recurrent disease phenotype.
EZH2 inhibition remodels the inflammatory senescence-associated secretory phenotype to potentiate pancreatic cancer immune surveillance
Authors: Chibaya Loretah , Murphy Katherine C. , Lopez-Diaz Yvette , DeMarco Kelly D. , Liu Haibo , Gopalan Sneha , Faulkner Melissa , Li Junhui , Morris John P. , Ho Yu-jui , Simon Janelle , Luan Wei , Kulick Amanda , de Stanchina Elisa , Simin Karl , Zhu Lihua Julie , Fazzio Thomas G. , Lowe Scott W. , Ruscetti Marcus 
Published: June 2022

ABSTRACTT cell-activating immunotherapies that produce durable and even curative responses in some malignancies have failed in pancreatic ductal adenocarcinoma (PDAC) due to rampant immune suppression and poor tumor immunogenicity. We and others have demonstrated that induction of cellular senescence and its accompanying senescence-associated secretory phenotype (SASP) can be an effective approach to activate not only T cell but also cytotoxic Natural Killer (NK) cell-mediated anti-tumor immunity. Here we found that the pancreas tumor microenvironment (TME) suppresses NK and T cell surveillance following therapy-induced senescence through EZH2-mediated repression of pro-inflammatory SASP genes. Genetic or pharmacological inhibition of EZH2 or its methyltransferase activity stimulated the production of pro-inflammatory SASP chemokines CCL2 and CXCL9/10 that led to enhanced NK and T cell infiltration and tumor eradication in preclinical PDAC mouse models. EZH2 activity was also associated with suppression of SASP-associated inflammatory chemokines and cytotoxic lymphocyte immunity and reduced overall survival in a PDAC patient cohort. These results demonstrate that EZH2 mediates epigenetic repression of the pro-inflammatory SASP in the pancreas TME, and that EZH2 blockade in combination with senescence-inducing therapies could be a powerful means to potentiate NK and T cell surveillance in PDAC to achieve immune-mediated tumor control.
New Hydra genomes reveal conserved principles of hydrozoan transcriptional regulation
Authors: Cazet Jack F. , Siebert Stefan , Little Hannah Morris , Bertemes Philip , Primack Abby S. , Ladurner Peter , Achrainer Matthias , Fredriksen Mark T. , Moreland R. Travis , Singh Sumeeta , Zhang Suiyuan , Wolfsberg Tyra G. , Schnitzler Christine E. , Baxevanis Andreas D. , Simakov Oleg , Hobmayer Bert , Juliano Celina E. 
Published: June 2022

AbstractThe epithelial and interstitial stem cells of the freshwater polyp Hydra are the best characterized stem cell systems in any cnidarian, providing valuable insight into cell type evolution and the origin of stemness in animals. However, little is known about the transcriptional regulatory mechanisms that determine how these stem cells are maintained and how they give rise to their diverse differentiated progeny. To address such questions, a thorough understanding of transcriptional regulation in Hydra is needed. To this end, we generated extensive new resources for characterizing transcriptional regulation in Hydra, including new genome assemblies for Hydra oligactis and the AEP strain of Hydra vulgaris, an updated whole-animal single-cell RNA-seq atlas, and genome-wide maps of chromatin interactions, chromatin accessibility, sequence conservation, and histone modifications. These data revealed the existence of large chromatin interaction domains in the Hydra genome that likely influence transcriptional regulation in a manner distinct from topologically associating domains in bilaterians. We also uncovered the transcriptomic profiles of two previously molecularly uncharacterized cell types, isorhiza-containing nematocytes and somatic gonad ectoderm. We identified novel candidate regulators of cell-type-specific transcription, several of which have likely been conserved at least since the divergence of Hydra and the jellyfish Clytia hemisphaerica over 200 million years ago. The resources generated in this study, which collectively represent the most comprehensive characterization of transcriptional regulation in a cnidarian to date, are accessible through a newly created genome portal, available at research.nhgri.nih.gov/HydraAEP/.
A call for improving the Key Biodiversity Areas framework
Authors: Farooq Harith , Antonelli Alexandre , Faurby Søren 
Published: June 2022

AbstractEight percent of all land surface has been designated as “Key Biodiversity Areas” (KBAs). Since these areas were established based on only two percent of all terrestrial species estimated to exist, we ask what would happen if we used all species on Earth to identify additional KBAs. We explore this question at a global scale by using data from 64,110 species of animals and plants to identify how many areas could qualify as KBAs under current criteria. We find that between 26% and 68% of the world’s terrestrial areas can be classified as KBAs, depending on the spatial resolution. The total area from potential KBAs increases drastically as more species are assessed, suggesting that if all species were included, virtually all land surface could eventually meet the biological requirements for becoming a KBA. In addition, KBAs lack a data-driven ranking system, thus assuming that all KBAs are equally worthy protection. KBAs are intended to be areas which are both of biological importance and manageable but the current approach largely sidesteps the biological component. We make an urgent call for stricter criteria in the KBA methodology or alternative methodologies that allow for biologically robust area prioritization, help secure evidence-based investments, and support progress towards the targets under the new Global Biodiversity Framework.