bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toxoplasma gondii’s basal complex: the other apicomplexan business end is multifunctional
Authors: Gubbels Marc-Jan , Ferguson David J. P. , Saha Sudeshna , Romano Julia D. , Chavan Suyog , Primo Vincent A. , Michaud Cynthia , Coppens Isabelle , Engelberg Klemens 
Published: February 2022

AbstractThe Apicomplexa are famously named for their apical complex, a constellation of organelles at their apical end dedicated to invasion of their host cells. In contrast, at the other end of the cell, the basal complex (BC) has been in the limelight since it is much less prominent and specific functions were not immediately obvious. However, in the past decade a staggering array of functions have been associated with the BC, and strides have been made in understanding its structure. Here, these collective insights are supplemented with new data to provide an overview of the understanding of the BC in Toxoplasma gondii. The emerging picture is that the BC is a dynamic and multifunctional complex, with a series of (putative) functions. The BC has multiple roles in cell division: it is the site where building blocks are added to the cytoskeleton scaffold; it exerts a two-step stretch and constriction mechanism as contractile ring; and it is key in organelle division. Furthermore, the BC has numerous putative roles in ‘import’, such as the recycling of mother cell remnants, the acquisition of host-derived vesicles, possibly the uptake of lipids derived from the extracellular medium, and the endocytosis of micronemal proteins. The latter process ties the BC to motility, whereas an additional role in motility is conferred by Myosin C. Furthermore, the BC acts on the assembly and/or function of the intravacuolar network, which may directly or indirectly contribute to the establishment of chronic tissue cysts. Here we provide experimental support for molecules acting in several of these processes, and identify several new BC proteins critical to maintaining the cytoplasmic bridge between divided parasites. However, the dispensable nature of many BC components leaves many questions unanswered regarding its function. In conclusion, the BC in T. gondii is a dynamic and multifunctional structure at the posterior end of the parasite.
Galaxy-SynBioCAD: Automated Pipeline for Synthetic Biology Design and Engineering
Authors: Hérisson Joan , Duigou Thomas , du Lac Melchior , Bazi-Kabbaj Kenza , Azad Mahnaz Sabeti , Buldum Gizem , Telle Olivier , El-Moubayed Yorgo , Carbonell Pablo , Swainston Neil , Zulkower Valentin , Kushwaha Manish , Baldwin Geoff S. , Faulon Jean-Loup 
Published: February 2022

AbstractWe introduce the Galaxy-SynBioCAD portal, the first toolshed for synthetic biology, metabolic engineering, and industrial biotechnology. The tools and workflows currently shared on the portal enables one to build libraries of strains producing desired chemical targets covering an end-to-end metabolic pathway design and engineering process from the selection of strains and targets, the design of DNA parts to be assembled, to the generation of scripts driving liquid handlers for plasmid assembly and strain transformations. Standard formats like SBML and SBOL are used throughout to enforce the compatibility of the tools. In a study carried out at four different sites, we illustrate the link between pathway design and engineering with the building of a library ofE. colilycopene-producing strains. We also benchmarked our workflows on literature and expert validated pathways. Overall, we find an 83% success rate in retrieving the validated pathways among the top 10 pathways generated by the workflows.
BET-independent MLV integration is retargeted in vivo and selects distinct genomic elements for lymphomagenesis
Authors: Nombela Ivan , Michiels Martine , Looveren Dominique Van , Marcelis Lukas , Ashkar Sara el , Belle Siska Van , Bruggemans Anne , Tousseyn Thomas , Schwaller Jürg , Christ Frauke , Gijsbers Rik , Rijck Jan De , Debyser Zeger 
Published: February 2022

ABSTRACTMoloney murine leukemia virus (MLV) infects BALB/c mice and induces T-cell lymphoma in mice. Retroviral integration is mediated by the interaction of the MLV integrase (IN) with members of the bromodomain and extra-terminal motif (BET) protein family (BRD2, BRD3 and BRD4). Introduction of the W390A mutation in MLV IN abolishes BET interaction. Here we compared the replication of W390A MLV and WT MLV in adult BALB/c mice to study the role of BET proteins in replication, integration and tumorigenesis in vivo. Comparing WT and W390A MLV infection revealed similar viral loads in blood, thymus and spleen cells. Interestingly, W390A MLV integration was retargeted away from GC-enriched genomic regions. However, both WT MLV and W390A MLV developed T cell lymphoma after a similar latency represented by an enlarged thymus and spleen and multi-organ tumor infiltration. Integration site sequencing from splenic tumor cells revealed clonal expansion in all WT MLV- and W390A MLV-infected mice. However, the integration profile of W390A MLV and WT MLV differed significantly. Integrations were enriched in enhancers and promoters but compared to WT, W390A MLV integrated less frequently into enhancers and more into oncogene bodies, such as Notch1 and Ppp1r16b. We conclude that host factors direct MLV in vivo integration site selection. Although, BET proteins target WT MLV integration preferentially towards enhancers and promoters, insertional lymphomagenesis can occur independently from BET, likely due to the intrinsically strong enhancer/promoter of the MLV LTR.
Transertion is used for localized expression and assembly of Vibrio parahaemolyticus T3SS2
Authors: Kaval Karan Gautam , Chimalapati Suneeta , Siegel Sara , Rodriguez Nalleli Garcia , Dalia Ankur B. , Orth Kim 
Published: February 2022

AbstractUsing environmental cues, bacteria commit to the assembly of transmembrane complexes such as the type III secretion system 2 (T3SS2), a membrane-bound, syringe-like secretory apparatus used during infection to inject host cells with virulence factors. Here we report Vibrio parahaemolyticus uses transertion, localized transcription, translation, and membrane insertion, to assemble its T3SS2. Upon binding bile acids, the membrane bound receptor and transcription factor VtrA/VtrC captures the T3SS2 pathogenicity island at the inner membrane. Activated VtrA/VtrC induces production of VtrB, the membrane bound master T3SS2 transcriptional regulator. VtrB then induces the membrane-proximal T3SS2 genes to undergo transertion for assembly of the membrane inserted secretion machinery. Transertion is a process that can be used for the efficient assembly of membrane-bound molecular complexes in response to extracellular signals.One-Sentence SummaryLocalized transcription, translation, and membrane insertion of multi-protein complexes in bacteria in response to host cues.
AN IMAGING FLOW CYTOMETRY-BASED METHODOLOGY FOR THE ANALYSIS OF SINGLE EXTRACELLULAR VESICLES IN UNPROCESSED HUMAN PLASMA
Authors: Woud Wouter W. , van der Pol Edwin , Mul Erik , Hoogduijn Martin J. , Baan Carla C. , Boer Karin , Merino Ana 
Published: February 2022

AbstractExtracellular vesicles (EVs) are tissue-specific particles released by cells containing valuable diagnostic information in the form of various biomolecules. To rule out selection bias or introduction of artefacts caused by EV isolation techniques, we present a clinically feasible, imaging flow cytometry (IFCM)–based methodology to phenotype and determine the concentration of EVs with a diameter ≤400 nm in human platelet-poor plasma (PPP)withoutprior isolation of EVs.Instrument calibration (both size and fluorescence) were performed with commercial polystyrene beads. Detergent treatment of EVs was performed to discriminate true vesicular events from artefacts. Using a combination of markers (CFSE & Tetraspanins, or CD9 & CD31) we found that >90% of double-positive fluorescent events represented single EVs.Through this work, we provide a framework that will allow the application of IFCM for EV analysis in peripheral blood plasma in a plethora of experimental and potentially diagnostic settings. Additionally, this direct approach for EV analysis will enable researchers to explore new corners of EV as cellular messengers in healthy and pathological conditions.
COVID-19 and privacy: a European dilemma
Authors: Renda Andrea 
Published: February 2022

Purpose
This paper aims at discussing the options available to governments when it comes to the use of technology to contain the spread of the COVID-19 virus.

Design/methodology/approach
This is an opinion piece, based on very recent developments (COVID-19), and based on a well-known trade-off between privacy and state surveillance, especially in times of crisis that threaten the survival of a nation.

Findings
The main finding is that technology alone will not help, and there are several reasons to doubt that the recently proposed European system to track the contagion in a privacy-preserving way (pan-European privacy preserving proximity tracing [PEPP-PT]) would be a fully effective solution.

Research limitations/implications
This is a short paper, which is very dependent on current developments. It was written in a very short time, so the level of depth in the references to the literature and the caselaw is limited. The main implication is that this paper is very far from the final word in the analysis of the interplay between technology and society, especially in democratic countries.

Practical implications
There is a need to ensure that the temporary measures that will be adopted during the pandemic do not extend to the post-COVID-19 period.

Originality/value
To the best of the author’s knowledge, this is a very fresh debate; the paper is thus original and proposes one of the first structured comments to the PEPP-PT and DP-3T conceptual designs.
Framework proposal for BIM implementation in Brazilian construction and development companies
Authors: Sena Tito Ceci de , Fabricio Márcio Minto 
Published: February 2022

PurposeThis study proposes a framework for collaborative building information modeling BIM implementation in construction and development companies in the Brazilian architecture, engineering and construction (AEC) market. The study addresses aspects concerning BIM collaboration, levels of adoption and maturity, classification of BIM objects and use of tools.Design/methodology/approachThe study conclusions are based on a bibliographic review and on active participation in a BIM implementation process conducted with two construction and development companies that participated in the study, which allowed examining the practical problems of the elaboration of BIM in various technical specialties, and the proposition of a framework to help overcome these limitations.FindingsThe research identified the importance of adopting standardized methods to develop models, establishing common classifications for objects to allow the use by different stakeholders on 3D, 4D and 5D processes, in a context that information is scattered and, in many cases, divergent across different companies and even different areas from the same company.Originality/valueThe study presents a practical set of methods and tools to be used within a context common to the Brazilian AEC market, on which construction and development companies are responsible for the management of the design and construction phase of a building. The recommendations of the research take into account the shortage of nationwide frameworks and classification standards, so it contributes to filling some gaps of current literature that cover theoretical aspects of guidance documents for BIM implementation but do not detail specific practical applications within a determined context. The limitations of the framework proposed are its focus on establishing context-specific guidelines, which may not be suitable universally.
Corporate governance and corporate social responsibility: new evidence from China
Authors: Aboud Ahmed , Yang Xinming 
Published: February 2022

PurposeThis paper aims to examine the impact of corporate governance on corporate social responsibility (CSR) performance, paying particular attention to modern Chinese businesses. Particularly, it examines how ownership concentration, boards of directors and boards of supervisors affect the quality of CSR performance.Design/methodology/approachThis study employs the regression analysis using a sample from listed companies in Shanghai and Stock Exchanges covering 2014 until 2018.FindingsUsing a sample of listed companies in Shanghai and Stock Exchanges, the empirical evidence, A-share listed companies between 2014 and 2018, this empirical investigation demonstrates that corporate governance does indeed have a significant effect on CSR. However, various types of corporate governance mechanisms have differing effects on CSR. The authors find that ownership concentration has a positive impact on CSR performance, while the size of a company’s board of supervisors has a positive impact on CSR performance. By doing so, the authors provide practical implications to users, and regulatory authorities to make better decisionsOriginality/valueThis paper contributes to the existing literature by examining the impact of corporate governance on a company’s abilities to meet its CSR objectives in China. Much of the empirical studies on this issue are centred on the Western world, notably Western Europe and the USA.
The effect of generalist CEOs on social novelty in the restaurant industry
Authors: Park Sungbeen , Lee Seoki 
Published: February 2022

PurposeThrough the lens of the upper echelons theory, this study aims to investigate how generalist chief executive officers (CEOs) affect social novelty. This paper also explores the moderating effect of CEO power on the relationship between generalist CEOs and social novelty.Design/methodology/approachThis study uses generalized estimating equation models and robust standard errors by firm to correct for autoregressive disturbances within clusters in the data.FindingsRestaurant firms with generalist CEOs are likely to feature gender diversity and member change in the top management structure. This positive effect of a generalist CEO on top management team’s (TMT) structure is enhanced by the CEO’s power over board members.Practical implicationsThis study presents important evidence that CEOs’ characteristics largely influence the gender heterogeneity and dynamic of the TMT, which in turn promote and shape innovative initiatives and actions.Originality/valueTo the best of the authors’ knowledge, this paper is one of the first to investigate the effect of CEOs’ human capital on the way in which the TMT is composed and characterized in the restaurant sector.
Cocreating food experience “delivered” from iconic local restaurants
Authors: Leung Xi Yu , Wang Xingyu , Levitt Jamie Alexander , Lu Lu 
Published: February 2022

Purpose
This study aims to explore an emerging phenomenon – gourmet meal kits delivered to out-of-towners from iconic local restaurants. This study explores the interplay of goal framing and basic needs and their effects on customer intentions. This study also examines the underlying mechanism of experience cocreation and the moderating effect of food involvement.

Design/methodology/approach
This study develops a framework that combines the self-determination theory (SDT) and customer experience cocreation. Two online experimental studies involving 478 US consumers were conducted to examine the underlying mechanism of customers’ decision-making on purchasing gourmet meal kits (Study 1) and visiting destination restaurants after receiving the meal kit (Study 2).

Findings
Intrinsic goal-framing leads to stronger intentions to purchase gourmet meal kits and to visit destination restaurants that sell meal kits. In contrast, extrinsic goal-framing enhances the positive influence of perceived relatedness and competence on behavioral intentions. The effects of goal framing and basic needs on behavioral intentions are moderated by food involvement and mediated by experience cocreation to varying degrees.

Originality/value
To the best of the authors’ knowledge, this study is among the first to empirically examine an emerging business model – delivering restaurants’ food experiences to out-of-towners. The study also expands the application of the SDT by incorporating customer cocreation as the underlying mechanism.
SaaS or not: optimal versioning strategy of releasing enterprise software
Authors: Sun Yangyang , Dang Chuangyin , Feng Gengzhong 
Published: February 2022

PurposeWith the rapid development of cloud computing, most software firms face the significant choice of whether they should change the versioning strategy of enterprise software from releasing the on-premise version to the software-as-a-service (SaaS) version. Data being generated and hosted on SaaS vendors' servers brings multiple effects. It enables customers to enjoy the flexibility of accessing data and using the software remotely, named the “portability” effect. However, on the other hand, the cumulative data resources on the cloud also provide a clear target for external attacks, leading to the concern of information security. Considering these, the authors hope to offer insights for software firms by exploring the strategy selection problem.Design/methodology/approachTaking the portability effect and security risks of the SaaS licensing model into account, the authors develop a two-period model to figure out the market segmentation and identify the feasible conditions for employing three alternative strategies. Comparative statics analyses are conducted to explore the influencing mechanism of exogenous factors on strategy selection. The authors also discuss the strategy selection in the presence of the network effect and the security loss faced by users of on-premise software.FindingsOne significant finding is that the on-premise strategy can be excluded when the potential loss from security risks is small. Under this circumstance, the dual version strategy is optimal provided that the increase of customer valuation caused by portability effect is below a threshold. Otherwise, the SaaS strategy generates the highest profit. When the potential loss from security risks turns large, the on-premise strategy, the dual version strategy and the SaaS strategy are the optimal options in order as the portability effect on customer valuation gets stronger.Originality/valuePrevious literature has insufficiently addressed the versioning issue of enterprise software. In this paper, the distinctive features of the SaaS model are considered, and differentiated results compared with previous work are obtained. The research results provide guidelines for software firms in deciding their product releases in the future.
Linking servicescape and experiencescape: creating a collective focus for the service industry
Authors: Kandampully Jay , Bilgihan Anil , Amer Sally Mohamed 
Published: February 2022

PurposeThe purpose of this paper is to review what one knows – and does not know about servicescape and experiencescape. The paper provides a comprehensive conceptualization, discussion of the servicescape and experiencescape and calls for the need for a collective focus of servicescape and experiencescape for the service industry.Design/methodology/approachThis conceptual paper analyzes multiple literature studies related to servicescape, experiencescape and other related concepts such as service quality and customer experience for building a comprehensive framework that draws knowledge from both servicescape and experiencescape in an effort to create a collective focus.FindingsPrior research on the components of servicescape and experiencescape raises more questions than answers. The findings of this study highlight the importance of technology as one of the key components of experiencescape to motivate customers to engage in the consumption environment. Furthermore, the proposed conceptual framework provides a detailed discussion and highlights the importance of using servicescape and experiencescape concepts together as a collective framework to bring about greater benefits to the service industry. The proposed framework suggests that these concepts are not only collective but also interrelated.Research limitations/implicationsTechnology is not a tool but an important partner for the firm to create the experience. It can bring together marketing, management and operations within the organization to collectively focus on the customer. Collectively designed servicescape and experiencescape will create lasting memories and emotional connections with customers.Practical implicationsService organizations can develop smart experiencescapes that positively influence customer value cocreation and heighten customer experience by utilizing technology. Managers are advised to understand consumers' emphasis on technological personalization, aesthetics, functionality, interactivity and social presence while participating in cocreation. Technologies may improve the experience by incorporating real-time and less-restrained interactions between consumers and the service organization.Originality/valueThis paper synthesizes insights from the extant literature related to servicescape, service quality, customer experience and experiencescape. Further, it helps to extend the current understanding of experiencescape and calls for the need to incorporate technology as one of the key experience components in the experiencescape concept. Furthermore, this study highlights the importance and the need to bring these two concepts together with a collective focus to enhance value for the customer. Thus, it is argued here that the collective focus of servicescape and experiencescape in the service industry will create new opportunities for further research and practical applications.
Customer interaction strategy, brand purpose and brand communities
Authors: Calder Bobby J. 
Published: February 2022

PurposeCustomer interactions are integral to service brands. Indeed, many product brands have added services in order to create more opportunity for customer interaction. This paper deals with the strategic use of customer interactions to build a strong brand. Customer interaction strategy has evolved considerably beyond traditional sales and advertising in how customer interactions are conducted. Yet the Pareto rule (e.g. 80% of sales from 20% of customers) continues to guide who brands interact with. Conventional wisdom is that firms should target heavy users or customers high on the recency, frequency and monetary (RFM) value of purchases. The purpose of this conceptual paper is to examine how brands can use brand purpose and brand communities to target light users and even nonuser stakeholders as well.Design/methodology/approachThere is reason to hypothesize that brands can go beyond the heavy user limit of targeting customer interactions by developing brands around purpose. Purpose can be based on engagement with personal goals or values or on societal values about doing good for others. Brand purpose can be reinforced by either life purpose brand communities or societal purpose brand communities.FindingsThe paper reviews findings in the brand interaction, brand purpose and brand communities literature.Research limitations/implicationsThe two types of brand purposes and brand communities offer the possibility of growing a brand by targeting customer interactions at light users and nonuser stakeholders.Practical implicationsThere are important future research issues to be addressed, but this approach could increase the value of brands to consumers and extend the life cycle of brands for organizations.Social implicationsFurthermore, the concept of societal brand purpose could allow firms to focus on creating shareholder value as well as addressing social and environmental problems.Originality/valueThis paper broadens the current conception of customer interaction strategy and is thus relevant to relationship and experience marketing.