bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Alpha-fetoprotein Promotes Hepatocellular Carcinoma Progression by Negatively Regulating Hippo Signaling Pathway Running title: AFP Regulate the Hippo Signaling Pathway
Authors: Leng Guangxian , Wu Fahong , Gong Hongxia , Liu Guiyuan , Wei Hangzhi , Guo Liuqing , Ma Hanwei , Kong Yin , Wang Mancai , Zhang Youcheng 
Published: June 2022

Abstract
Background: Alpha-fetoprotein (AFP) is a specific tumor marker for hepatocellular carcinoma (HCC) and promotes tumor cell proliferation in HCC, but the mechanism is not completely clear. This research aimed to supplement the mechanism of AFP in HCC. Methods: Lentivirus transfected HCC cells for AFP knockdown and overexpression, CCK-8, clone formation, scratch assay, and 3D multicellular tumor spheroids（MCTS） formation assay verified the positive regulation of AFP on HCC. Co-immunoprecipitation, liquid chromatography-mass spectrometry (LC-MS/ MS), and bioinformatics techniques were used to identify proteins interacting with AFP and related pathways in HCC cells, and western blot, real-time quantitative PCR(RT-qPCR), and immunofluorescence were used to detect the expression changes of proteins associated with AFP pathways. The effect of AFP on the HCC cell cycle was detected by flow cytometry. Results: AFP promotes the proliferation, migration, and the formation of MCTS of HCC cells. In HCC, AFP interacts with large tumor suppressor 2 (LATS2) and negatively regulates Hippo signaling pathway. AFP inhibits the phosphorylation of LATS2, which in turn inhibits the phosphorylation of yes associated protein (YAP). In addition, AFP was positively correlated with the expression of CyclinD1. Conclusion: AFP negatively regulates Hippo signaling pathway through the LATS2/YAP axis and promotes the proliferation, migration, and formation of MCTS of HCC cells.
Whole-Transcriptome RNA Sequencing Reveals the Molecular Responses and C eRNA Regulatory Network under Simulated Long-term Spaceflight Composite Stress in Hippocampus
Authors: Yin Yishu , Liu Junlian , Xu Chong , Zeng Deyong , Wu Xiaorui , Zhu Yuanbing , Fan Quanchun , Zhao Shuang , Wang Jiaping , Liu Yu , Jiao Lei , Li Yongzhi 
Published: June 2022

Abstract
To systematically evaluate the affect of simulated long-term spaceflight composite stress (LSCS) in hippocampus and to gain more insights into the transcriptomic landscape and molecular mechanism, we performed whole-transcriptome sequencing based on the control group (Ctrl) and the simulated long-term spaceflight composite stress group (LSCS) from six hippocampus of rats. Subsequently, differential expression analysis was performed on the Ctrl and LSCS groups, followed by enrichment analysis and functional interaction prediction analysis to investigate gene-regulatory circuits in LSCS. In addition, competitive endogenous RNA (ceRNA) network was constructed to gain insights into genetic interaction. The result showed that 276 differentially expressed messenger RNAs (DEmRNAs), 139 differentially expressed long non-coding RNAs (DElncRNAs), 103 differentially expressed circular RNAs (DEcircRNAs), and 52 differentially expressed microRNAs (DEmiRNAs) were found in LSCS samples compared with the controls, which were then subjected to enrichment analysis of Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways to find potential functions. PI3K-Akt signaling pathway and MAPK signaling pathway may play fundamental roles in the pathogenesis of LSCS. A ceRNA network was constructed with the predicted 340 DE pairs, which revealed the interaction roles of 220 DEmiRNA-DEmRNA pairs, 76 DEmiRNA-DElncRNA pairs, and 44 DEmiRNA-DEcircRNA pairs. Further, Thrombospondins2 was found to be a key target among those ceRNAs. Overall, we conducted for the first time a full transcriptomic analysis of the response of hippocampus to the LSCS that involved a potential ceRNA network, thus providing a basis to study the underlying mechanism of the LSCS.
Using End-to-End imbalanced Temporal Deep Gaussian Process (iTDGP) to Predict Acute Ischemic Stroke Lesions
Authors: Soltanpour Mohsen , Yousefnezhad Muhammad , Greiner Russ , Boulanger Pierre , Buck Brian 
Published: June 2022

Abstract
As one of the leading causes of mortality and disability worldwide, Acute Ischemic Stroke (AIS) occurs when the blood supply to the brain is suddenly interrupted because of a blocked artery. Within seconds of AIS onset, the brain cells surrounding the blocked artery die. The automated and precise prediction of AIS lesions plays a vital role in the treatment planning and prevention of further injuries. Unfortunately, the current standard AIS assessment method, which thresholds the 3D measurement maps extracted from Computed Tomography Perfusion (CTP) images, is not accurate enough. Due to this fact, in this article, we propose the imbalanced Temporal Deep Gaussian Process (iTDGP), a probabilistic model that can improve AIS lesions prediction by using baseline CTP time series. Our proposed model can effectively extract temporal information from the CTP time series and map it to the class labels of the brain's voxels. In addition, by using batch training and voxel-level analysis iTDGP can learn from a few patients and it is robust against imbalanced classes. Moreover, our model incorporates a post-processor capable of improving prediction accuracy using spatial information. Our comprehensive experiments, on the ISLES 2018 and the University of Alberta Hospital (UAH) datasets (including 63 and 100 patients respectively), show that iTDGP performs better than state-of-the-art AIS lesion predictors, obtaining the (cross-validation) Dice score of 71.42% and 65.37% with a significant p<0.05, respectively.
Readability Assessment of Online Men's Health Education Materials from the Sexual Medicine Society of North America, Urology Care Foundation, and European Association of Urology.
Authors: Callegari Michael , Pominville Raymond , Zhou Christopher , Piyevsky Brandon , Jesse Erin , Prunty Megan , Loeb Aram , Thirumavalavan Nannan , Ghayda Ramy Abou 
Published: June 2022

Abstract
Introduction: The use of online health information by patients has gained considerable attention during the last decade. The National Institute of Health (NIH) and American Medical Association (AMA) advocate the dissemination of accessible and readable resources. We aimed to evaluate the readability of online patient resources from the Sexual Medicine Society of North America (SMSNA) website.Methods: Content published under the web-section “SMSNA: For Patients - conditions and topics'' portal (n=16) were reviewed. Identical topics published online via Urology Care Foundation (ie AUA; n=8) and the European Association of Urology (ie EAU; n=8) were also reviewed. Readability and estimated educational level required for understanding was assessed using validated readability and English language assessment tools including the Flesh Reading Ease score, Flesch Kincade, Gunning Fog, Simple Measure of Gobbledygook (SMOG), Coleman-Liau and Automated Readability index.Results: SMSNA online patient education materials (PEM), on average, are written at a college sophomore reading level (14th grade). This is 6.5 and 8 grade levels higher than the average U.S. adult and recommended reading levels for PEM, respectively. Comparable AUA and EAU resources were an average of 10th (p<0.05) and 12th (p=0.854) grade reading levels, respectively.Conclusion: Men’s sexual health PEM’s published online are written at an advanced reading level, most notably the SMSNA compared to equivalent professional sources. This could pose a barrier to patient understanding and impact patients’ engagement and health decision-making. One proposed mitigatory strategy is for PEM to provide improved readability, and appropriate user-friendly language to facilitate easier and inclusive understanding, outreach and educational support.
The practice of COVID-19 prevention strategy post vaccination among health professionals in Felege Hiwot Referral Hospital, Northwest Ethiopia
Authors: Engdaw Garedew Tadege , Worede Eshetu Abera , Destaw Bikes 
Published: June 2022

Abstract
Background
Corona Virus Disease is an acute respiratory illness first discovered and identified in China. Countries are taking various precautions to prevent COVID-19 in accordance with WHO guidelines.
Objective
The objective of this study is to assess the practice of the COVID-19 prevention strategy post vaccination and associated factors among health professionals in Felege Hiwot Referral Hospital, Bahir Dar, Northwest Ethiopia, in 2021.
Methods
An institutional-based cross-sectional study was carried out at Felege Hiwot Referral Hospital, Bahir Dar Ethiopia.
Results
In this study, 68.7% (95% CI: (63.7, 73.8)) of health care professionals had good practice of the COVID-19 prevention strategy post vaccination. Sex, 1.76% (95% CI: (1.08, 2.89), Marital status (Married) 1.75% (95% CI: (1.09, 2.93)), and good attitude towards vaccination 3.42% (95% CI: (2.13, 5.48)) were significantly associated with the practices of health care professionals post vaccination.
Conclusions
The practices of COVID-19 preventive strategies post vaccination was good among healthcare professionals.
Dielectric Characterization and Statistical Analysis of Ex-Vivo Burnt Human Skin Samples For Microwave Sensor Development
Authors: Rangaiah PRAMOD K B , Kouki Mokhtar , Dhouibi Yasmina , Huss Fredrik , Mandal Bappaditya , Augustine Bobins , Perez Mauricio D , Augustine Robin 
Published: June 2022

Abstract
Precise data acquisition, characterization, and finding the relationship between permittivity variations of human skin with degree of burns is critically important because It further helps in the development of excellent sensor devices for diagnostics and treatment of patients suffering from burns and scalds. This work is also a part of the European “Senseburn” project that focuses to develop a non-invasive diagnostic tool for the assessment of human burns based on its degree and depth in the clinical setup. In this work, several Ex-vivo burnt samples were collected from the Uppsala University Hospital (Akademiska sjukhuset, Sweden), and out of that, eight samples with different burn degrees and of various human body parts were selected for the analysis. The dielectric characterization of the categorized samples was done by using an open-end co-axial probe kit. The measurement was made systematically and clinician feedback forms were maintained throughout the process. The measurement data followed the FASTCLUS procedure which was analyzed initially using density plot, Convergence, and cubic clustering criteria respectively. The dielectric characterization was made from 500 MHz to 10 GHz with 1001 points and from the previous sensor designs, the results were found to be excellent between 500 MHz to 5 GHz. For the statistical analysis, 11 frequency points were considered for 8 samples. The results of the basic statistical analysis using the FASTCLUS procedure resulted in 88 data sets. Later, data sets were analyzed based on the cluster-wise of all samples and sample-wise clusters. Every sample was made with two clusters i.e, cluster 1 which consisted of healthy sectors, and cluster 2 which consisted of burnt sectors. Furthermore, we found that the permittivity differences of clusters are proportional to the degree of the burns. This is pivotal information and It helps to improve the functioning of the diagnostic microwave sensors by designing them according to the permittivity variations. For this purpose, an extensive campaign of around 1000 measurements across a band of 1–30 GHz was done and it leads to the conclusion that each skin region of interest (ROI) provides unique dielectric properties.
Association between plasma-free hemoglobin levels, lactate dehydrogenase and hemolysis-index in patients with and without mechanical circuit support - a retrospective data analysis
Authors: Zapletal Bernhard , Zimpfer Daniel , Schloeglhofer Thomas , Fritzer-Szekeres Monika , Szekeres Thomas , Bernardi Martin Herrmann , Kainz Michael , Neugebauer Thomas , Tschernko Edda Maria 
Published: June 2022

Abstract
Purpose
Hemolysis is a frequent problem in patients with mechanical circulatory support (MCS) that can indicate device malfunctions or pump thrombosis. In this retrospective cohort study, we assessed whether hemolysis index (HI) can replace plasma free hemoglobin (fHb) and lactate dehydrogenase (LDH) to guide anticoagulation and performed a cost analysis to combine medical with economical efficacy.
Methods
We correlated simultaneously obtained HI, fHb, LDH measures of venous blood samples in a cohort of 96 ICU patients. 429 datasets (40.7%) were collected from patients with and 556 datasets (52.8%) without extracorporeal life support (ECLS). 68 (6.5%) from a cohort of 51 outpatients on LVAD support. A cost analysis of all three hemolysis monitoring options was performed.
Results
Correlation of HI and fHb values was highly significant (r = 0.968, p < 0.01) overall and equally significant (r = 0.949, p < 0.01) in samples with clinically significant hemolysis (HI ≥ 20). Correlation of fHb and LDH was low (r = 0.192, p < 0.01) in all patients. Estimated cost per ICU patient on ECLS was 0 USD if HI, 4.347.25 (794.21-5350.46) USD if fHb, 383.29 (70.02-471.74) USD if serial measurements of LDH were used for hemolysis monitoring.
Conclusions
HI represents a cost-efficient, accurate and easily obtainable alternative to fHb measurements for clinicians treating patients with and without MCS, whereas LDH is significantly less specific. Further clinical studies on major cohorts of short and long-term MCS patients are needed to assess the optimal HI target with minimal thromboembolic as well as bleeding events.
Effects of Nitrogen and Phosphorus on the Regulation of Germination, Seedling Growth and the Yield Traits of Perennial Cool-Season Forage Grasses
Authors: Chen Zhao , Niu Junpeng , Guo Zhipeng , Cao Xinlong , Yan Xuebing , Wang Quanzhen 
Published: June 2022

Abstract
Purpose Crop production in saline-alkali soils is often challenged by excessive fertilization, that can increase carbon equivalent emissions, and also by inadequate soil quality, thereby threatening sustainability. Previous researches on fertilization management focused on the short-term effects of single application of nitrogen (N) and phosphorus (P). Little is known about the long-standing effects that combined N and P fertilizers on seedling growth and crop yields. Thus, specific knowledge regarding the interactions between N and P fertilizers management practices targeting improved system sustainability on crop yield and quality.Methods Using combinations of 18 levels of N fertilizer and 16 levels of P fertilizer, we conducted multi-factorial, orthogonally-designed field experiments with large sample sizes for three years to determine the optimal ratio of N and P for germination, seed yield, seedling growth and yield components of six grasses. Results We calculated the mode of the physiological traits and used the thickest value as the optimum, and found a significantly antagonistic interaction between N and P for seedling growth traits, seed components and seed yields when the N: P ratio was between 2 and 3, with the optimal N: P ratio being 2.86:1. And the plants treated with high N and low P had higher values for physiological traits, seed components and seed yields, and strongly promoted vegetative/reproductive growth of seedlings. Conclusion These results can be applied to develop breeding strategies and quality detection in saline-alkali environments that contain soil with low availability of N and P.
Drag Reducing Nose Fairings for Existing Freight Train Locomotive
Authors: Stucki Chad L. , Maynes Daniel 
Published: June 2022

Abstract
At cruising speed, the largest contributing factor to train fuel consumption is aerodynamic drag, and the leading locomotive experiences much more drag than any other car in the train. This work reports on the drag reduction that can be realized by the use of add-on nose fairings that are deployed on leading locomotives in a train set. Two types of fairing shapes were considered and all fairing walls are flat. It is anticipated that the fairing shapes would result from the deployment of easily stowed panels in an origami inspired manner. One of the fairing shapes has the appearance of a wedge and the other fairing is also wedge shaped, but with flow directing side wall features. For each general fairing shape, the important dimensions were parametrically varied in a systematic manner to identify the dimensions that yield maximum drag reduction. For the first shape, 45 different scenarios were considered; for the second shape, 15 were considered. A steady commercial computational fluid dynamic solver was employed to solve the flow field and locomotive drag for each of the scenarios. The best performing wedge-shaped fairing reduced the leading locomotive drag by nominally 14% and the best performing fairing shape with the side walls reduced the drag by 17%.
Hsa_circ_0021727 (circ-CD44) promotes ESCC progression by targeting miR-23b-5p to activate the TAB1/NFκB pathway
Authors: Meng Fan , Zhang Xiaokang , Wang Yanting , Lin Jie , Tang Yulin , Zhang Guisheng , Qiu Binqiang , Zeng Xingdu , Liu Weiyou , He Xin 
Published: June 2022

Abstract
Background: The morbidity and mortality of esophageal squamous cell carcinoma (ESCC) remain high globally. Circular RNAs (circRNAs) play an important role in tumor progression. We discovered a novel aberrantly expressed circRNA (hsa_circ_0021727) in ESCC patients. However, the mechanism of hsa_circ_0021727 in tumors is still not elucidated. The purpose of this article is to investigate the biological role of hsa_circ_0021727 and its role in ESCC progression.Methods: Microarray analysis was used to screen diferentially expressed novel circRNAs.qRT-PCR,RNA-ISH and FISH were used to analyze the expression and localization of hsa_circ_0021727.MTT, colony formation, EdU, transwell assay, three-dimensional (3D) spheroid invasion assays and wound healing assays were used to describe biological effects on ESCC cells.Xenograft experiment and tail vein injection experiment were used to analyze tumor growth and metastasis in vivo.RNA-sequencing was used to identify hsa_circ_0021727 downstream targets.Western blot was used to detect the downstream protein expression of hsa_circ_0021727.RNA pulldown and dual luciferase experiments were used to evaluate the interaction of hsa_circ_0021727, miR-23b-5p and TAB1.Results: We screened a novel circRNA (hsa_circ_0021727) whose expression was upregulated in ESCC patients.ESCC patients with high expression of hsa_circ_0021727 had shorter survival time.Hsa_circ_0021727 promoted the proliferation, invasion and migration of ESCC cells. However, miR-23b-5p inhibited this ability of hsa_circ_0021727. MiR-23b-5p acts by targeting TAB1. In conclusion, hsa_circ_0021727 promoted ESCC progression by upregulating the expression of TAK1-binding protein 1(TAB1) via "sponge adsorption" of miR-23b-5p. In addition, in vivo experiments also confirmed that hsa_circ_0021727 can promote the proliferation, invasion and migration of ESCC cells.Conclusions：Hsa_circ_0021727 (circ_CD44) promotes ESCC progression by targeting miR-23b-5p to activate the TAB1/NFκB pathway.These findings may provide potential targets for the treatment of ESCC.
Identification of hub genes affecting triple-negative breast cancer using bioinformatics analysis strategies
Authors: Liu can , Kan Hongxing , Wang Yongkang , Gao Yong , Nie Yiqi , Zhang Yuxuan , Hu Jili 
Published: June 2022

Abstract
BackgroundBreast cancer is one of the most serious malignancies in women throughout the world, with an increasing number of people being diagnosed and dying from it. Triple-negative breast cancer (TNBC), which has a dismal prognosis, the estrogen receptor (ER), progesterone receptor (PR), and human growth factor receptor type 2 (Her2) immunohistochemistry test results are all negative. MethodsTo find differentially expressed genes (DEGs), two datasets from the Gene Expression Omnibus (GEO) were used to search for TNBC and not triple-negative breast cancer (not-TNBC) differentially expressed genes. Using weighted gene co-expression network analysis (WGCNA), the breast cancer data from the Cancer Genome Atlas (TCGA) was utilized to study the relationship between genes and TNBC features and to identify the hub genes in the most important linked modules. The Gene Ontology (GO) and Kyoto Encyclopedia of the Genes and Genomes (KEGG) enrichment analyses were performed on the differential genes in the GEO dataset and the hub genes in the TCGA dataset to study the nature of genes and to find gene enrichment pathways. A protein-protein interaction network (PPI) was created for each of the genes considered. To discover the most important genes in PPI networks, utilize the cytoHubba plugin in Cytoscape. Following that, the gene expression profiling interaction analysis (GEPIA) was used to confirm the hub gene's expression. Finally, data sets from the cBioPortal database were utilized for survival analysis. ResultsWe detected 7 genes that were up-regulated and 2 genes that were down-regulated by combining the GEO and TCGA databases.PGR, ELF5, AGR2, and GATA3 prolong life expectancy, while ESR1, TFF1, FOXA1, and AR shorten life expectancy.ConclusionOur study confirmed that PGR, ELF5, AGR2, GATA3, ESR1, TFF1, FOXA1, and AR may be potential therapeutic targets and biomarkers for TNBC.
Infection by the parasite Myxobolus bejeranoi (Cnidaria: Myxozoa) suppresses the immune system of hybrid tilapia
Authors: Maor-Landaw Keren , Smirnov Margarita , Brekhman Vera , Ofek-Lalzar Maya , Yahav Tal , Lotan Tamar 
Published: June 2022

Abstract
Background: Tilapia is the second most cultured fish worldwide and in Israel, it constitutes 60% of total production. Recently, the myxozoan parasite Myxobolus bejeranoi was found to infect the gills of hybrid tilapia (Nile tilapia (Oreochromis niloticus) X Jordan/Blue tilapia (O. aureus)), causing high morbidity and mortality. Myxozoa (Cnidaria) is a large group of microscopic obligate endoparasites that can cause emerging diseases, affecting wild fish populations and fisheries. Here, we aimed to elucidate the cellular processes occurring in the fish host following infection by M. bejeranoi. Methods: Comparative transcriptomics was performed on fish that had been exposed to infectious fish pond water containing M. bejeranoi actinospores for 24 hours. We compared the effects of minor, intermediate, and severe infection levels on the sporulation site, namely the gills, as well as on the hematopoietic organs head kidney and spleen. Enrichment analyses for Gene Ontology and KEGG pathways were performed and gene network was generated based on Jaccard distance matrix and visualized with Cytoscape. Results: Enrichment analysis indicated immune system activation in the gills at severe infection stage. However, in the head kidney a broad immune suppression was observed, including deactivation of secreted cytokines that play important roles in immune response of fish and of a transcription factor responsible for T helper cell differentiation. Moreover, the cytotoxic effector proteins perforin and granzyme B were down-regulated in the spleen. Finally, significant up-regulation of insulin in the spleen may imply that it functions as an immunomodulatory hormone inducing systemic immune suppression. Conclusions: Our analysis illustrates the incapacity of the hybrid tilapia host to eliminate its pathogenic invader, the myxozoan M. bejeranoi. We hypothesize that the signal for immune suppression originates at the sporulation site and traverses to the hematopoietic organs. These findings suggest that M. bejeranoi is a highly efficient parasite that disables the defense mechanisms of its fish host.