bluuebunny/crossref_metadata_2025_split huggingface dataset:
RUNX1A isoform is overexpressed in acute myeloid leukemia and is associated with FLT3 internal tandem duplications
Authors: Cumbo Cosimo , Tarantini Francesco , Parciante Elisa , Anelli Luisa , Zagaria Antonella , Biondi Giuseppina , Caratozzolo Mariano Francesco , Orsini Paola , Coccaro Nicoletta , Tota Giuseppina , Redavid Immacolata , Conserva Maria Rosa , Minervini Angela , Minervini Crescenzio Francesco , Gagliardi Vito Pier , Delia Mario , Marzano Flaviana , Telegrafo Claudia , Cox Sharon Natasha , Natalicchio Annalisa , Balech Bachir , Tullo Apollonia , Gentile Mattia , Giorgino Francesco , Specchia Giorgina , Musto Pellegrino , Albano Francesco 
Published: January 2025

Abstract

Background. RUNX1A is the shortest and least expressed of the RUNX1 three main isoforms (A, B, C); despite this, the leukemogenic role of its overexpression has been clearly described. Several studies have shown RUNX1A involvement in different blood cancers and pilot observations in acute leukemia have been reported.
Methods. In this context, we evaluated RUNX1 isoformsexpression in a cohort of acute myeloid leukemia (AML) patients and associated our data with significant AML clinical and biological parameters. A focus was performed on FLT3 mutated cases. Genome-wide methylation data from the TF-1 cell line were studied to investigate the possible role of epigenetic regulation in RUNX1 expression. To verify whether RUNX1Aupregulation is linked to a specific transcriptional profile, high-throughput RNA sequencing was conducted.
Results. At diagnosis, we found RUNX1A and RUNX1B overexpression,with higher median levels in thrombocytopenic cases. No difference was observed for RUNX1C. RUNX1A overexpression is higher in more immature AML phenotypes. According to the mutational profile, FLT3 internal tandem duplication (ITD) positive cases have the highest RUNX1A levels and the presence of FLT3-ITD was the only molecular variable able to influence RUNX1Aexpression. RUNX1A overexpression is disease-related, associated with a specific transcriptional profile, and reappears at relapse, with no clear kinetics except in FLT3-ITD cases.
Conclusions. Overall, we demonstrate RUNX1A overexpression in AML and its association with the FLT3-ITD molecular subtype. Our data shed light on the dark side of RUNX1 deregulation, paving the way for further investigations.
Ziziphus mauritiana Leaf Extract Induces ROS-dependent Apoptosis by Downregulation of CD81 in MDA- MB 231 Triple Negative Breast Cancer Cells
Authors: Lestari Dian Yuliartha , Mastutik Gondo , Mukono Indri Safitri 
Published: January 2025

Abstract

Background. Breast cancer (BC) is the most prevalent cancer among women, with triple-negative breast cancer (TNBC) representing a particularly aggressive subtype. Current treatments often have limited efficacy and significant toxicity, highlighting the need for alternative therapeutic strategies. Ziziphus mauritiana (ZM) leaf extract contains triterpenoids which have been proven to be used as anticancer agents, but have not been studied in TNBC cells.
Aim. To examine the triterpenoid content in ZM, especially BA and OA, ZM cytotoxicity, intracellular ROS levels, the amount of apoptosis, and CD81 expression on MDA-MB 231 cells after being given ZM.
Material and Methods. The extract was prepared using a sequential extraction method. The presence of triterpenoids was confirmed via HPLC and LC-MS analysis. The cytotoxicity of the extract was assessed using the CCK-8 assay, while flow cytometry was employed to analyze CD81 expression and ROS levels. Apoptosis was quantified using an Annexin V-FITC/PI detection kit.
Results. Based on HPLC, the BA content in ZM is 28.3 µg/ml, and the OA content is 13.4 µg/ml. ZM significantly reduced MDA-MB 231 cell viability by 28.28% at 400 µg/ml after 48 hours. Flow cytometry revealed a notable downregulation of CD81 expression (p < 0.0001) and an increase in intracellular ROS levels (p < 0.0001). Furthermore, ZM induced early and late apoptosis in treated cells, with increases of 2.34-fold and 2.66-fold, respectively.
Conclusion. ZM leaf extract exhibits potent cytotoxic effects against MDA-MB 231 through ROS-dependent apoptosis and CD81 downregulation, suggesting its potential as a novel therapeutic agent for TNBC treatment.
Scalable and sustainable DMF-free solid-phase synthesis of liraglutideby 1-tert-butyl-3-ethylcarbodiimide-mediated couplings and catch-and-release acylation and purification strategies
Authors: Pacini Lorenzo , Muthyala Manoj Kumar , Zitterbart Robert , Marder Oleg , Rovero Paolo , Papini Anna Maria 
Published: January 2025

Abstract

The growing need for sustainable practices in pharmaceutical manufacturing has stimulated advancements in peptide synthesis. This study focuses on applying green chemistry principles to the synthesis of the Glucagon-Like Peptide-1 analog liraglutide, using novel and sustainable solid-phase synthetic strategies. By adopting the safer coupling reagent 1-tert-butyl-3-ethylcarbodiimide (T-Bec®) in combination with eco-friendly binary solvents like dimethyl sulfoxide and butyl acetate, we demonstrated that it is possible to significantly reduce the environmental impact while maintaining high efficiency and quality of the synthesis. T-Bec® minimizes hazardous byproducts, such as hydrogen cyanide, and enhances solvent compatibility, achieving crude purities and yields comparable to conventional syntheses. Two synthetic strategies were developed for liraglutide production. The first strategy based on a “direct synthesis”, incorporating a lipidated lysine building block into the peptide sequence, achieving 86% HPLC purity after catch-and-release purification. The second strategy based on “catch-lipidation-and-release” approach, allowed to obtain the peptide precursor without the lipid moiety, which was later linked during a controlled lipidation step. This latter strategy yielded purities exceeding 90% and reduced reliance on preparative HPLC. These findings highlight the effectiveness of T-Bec® and green solvent systems to optimize scalable and sustainable SPPS processes. These methods improve resource efficiency and reduce environmental impact, to allow a viable pathway to produce therapeutic peptide ingredients like liraglutide. This work underscores the potential of green chemistry to align pharmaceutical innovation with environmental responsibility.
Dupilumab for a Novel Treatment for Necrobiosis Lipoidica: Case Report
Authors: Sato Jennifer , Monaco Hailey , Sato Ryan 
Published: January 2025

Background
This paper describes a case of necrobiosis lipoidica (NL) which was successfully treated with dupilumab. NL can be a difficult and chronic disease, often with limited treatment options. Dupilumab is a biologic typically used for atopic dermatitis, but given its novel pathway, it has been used in a number of other conditions.

Case Presentation
We describe a patient with inflammation on her legs, which was biopsied and consistent with NL. Treatments, including topical steroids, were started, but there was no improvement, therefore, dupilumab was discussed. She agreed with the treatment and dupilumab injections, and she reported a decrease in redness and improvement in her NL.

Conclusion
With this report, hopefully, there will be another option for the treatment of NL and different and novel uses for dupilumab.
Raising Awareness on The Impacts of Caesarean Section Birth Method to Children's Health: Nationwide Survey to Indonesian Married Woman
Authors: Fernando Darrell , Darus Febriansyah , Kinesya Edwin , Kartjito Melissa Stephanie , Wasito Erika , Dilantika Charisma , Basrowi Ray Wagiu 
Published: January 2025

Aims
This study seeks to determine the level of knowledge mothers have about CS and its health implications, such as gut dysbiosis, before and after a health promotion.

Methods
We analyze the awareness of Indonesian mothers through a cross-sectional survey before and after health promotion, utilizing digital health education through national parenting platforms, PrimaKu and TemanBumil.

Findings
A total of 769 participants completed both the pre-test and post-test. There is an increase of awareness (70% to 95%), total score (mean ± SD: 4.34 ± 0.99 to 4.83 ± 0.49; p-value 0.000), and correct answer for each question (p-value < 0.05), respectively from pre-test and post-test. Instagram posts (77.07%), webinar (38.9%), Instagram live (35.94%), and Tiktok videos (34.02%) are the most preferred to least preferred forms of content, respectively.

Conclusion
Digital health campaigns using social media and webinars had a beneficial impact on raising awareness among Indonesian mothers about the impact of CS on children's health.
Ageing Characteristics of Stir Cast Al-5GNPs-1SiC Novel Composite
Authors: Tiwari Amit 
Published: January 2025

Background:
Currently, aluminium alloys are extensively employed in many sectors
due to their desirable characteristics such as rigidity, low weight, appropriate heat conductivity,
and malleability. The alloy's mechanical characteristics will enhance as the solidification rate increases.
Given the potential of aluminium (Al) alloys and their composites for both light structural
and biomedical applications, it is wise to explore the development of a new Al component. Additionally,
it is necessary to monitor recent trends in the development of functional Al-alloy and
composite materials through an extensive patent search. Examining current patents in the relevant
field justifies the need to study this element.

Method:
The present paper reports the study on a novel aluminium composite cast developed
through a clean liquid metallurgical route, to assess the metallurgical, chemical, and mechanical
parameters using an optical microscope, EDX, EDS, and SEM analysis. The novel composite
Al-5GNPs-1SiC were prepared by stir casting technique at 600 rpm for 15 minutes. The solidified
ingots were subjected to heat treatment, such as ice water quenching, followed by ageing the
quenched novel aluminium composite at 100°C, 200°C, 300°C, and 400°C for two hours. For evaluation
of hardness, tensile strength and microstructure; the desired samples were prepared as per
usual standards. Graphene and silicon carbide reinforced aluminium composite were made in this
study, both reinforced particles are dispersed homogenously in the aluminium matrix, making
them an efficient reinforcing filer to avoid deformation.

Results:
The Al matrix with (5 wt. %) graphene and (1 wt. %) silicon carbide had 136 MPa yield
strength, 266 MPa ultimate strength, 15 (%) elongation, and 106 (VHN) at aged 200°C. Furthermore,
the morphological changes in the surface caused by ageing were analysed using a scanning
electron microscope, as is the fracture appearance of the shattered specimen exposed to tensile
strain. The results show that the silicon carbide particles do not have a higher impact in the phase
transformation process during composite solidification up to (1 wt. %) because they have no significant
influence on the novel composite phase structure. According to the SEM images, the dimples
and cleavages are increased with (5 wt %) of graphene particles added to the Al matrix. A
combination of ductile and brittle modes of fracture was identified during the tensile test of the
novel composite, according to fractography analysis performed on the samples using scanning
electron microscopy (SEM) pictures.

Conclusion:
Stir casting and quench ageing produce remarkable mechanical characteristics and
structural homogeneity in the Al-5GNPs-1SiC novel composite.
LANDSLIDE HAZARD MAPPING IN SIBOLANGIT DISTRICT, NORTH SUMATRA PROVINCE USING GEOGRAPHIC INFORMATION SYSTEMS
Authors: Panjaitan Frandica , Somantri Lili , Maruddani Rizki Feroza , Kartikasari Ika 
Published: January 2025

The purpose of this study was to find out and describe the use of GIS in mapping the level of landslide vulnerability in Sibolangit District, Deli Serdang Regency, North Sumatra. The model used referred to the estimation of the 2004 Puslittanak, as it utilizes parameters that are easy to measure, widely available, and in accordance with the territory of Indonesia. These parameters include rainfall, rock type, soil type, slope gradient, and land cover. The research methods used were overlay, scoring, and weighting. In the mapping process, each parameter had a score of classification multiplied by the weight of each parameter, then the results of the multiplication of the scores and weights were summed based on the suitability of their geographical location. The results of the study showed that the Sibolangit District area had the potential for landslides from low, medium to high levels. Based on the landslide disaster prediction model, the study area predominantly fell under the moderate susceptibility class, covering 15 villages. Additionally, 10 villages were identified with low vulnerability level and 5 villages with high vulnerability level. The use of GIS was also used to see the size of each area in Sibolangit District based on the level of vulnerability to landslide disasters. The level of validity of this map is quite good because it uses official data and methods suitable for the territory of Indonesia. Thus, it is accurate enough for the purpose of vulnerability identification and landslide disaster mitigation planning.
PENGARUH DOLOMIT TERHADAP SIFAT KIMIA TANAH, BAKTERI PELARUT FOSFAT, SERAPAN Ca, Mg, P DAN PRODUKSI JAGUNG MANIS
Authors: Nopriani Lenny Sri , Ramadhani Filka , Ishaq Rizki Maulana , Kurniawan Syahrul , Hidayat Muhamad Taufiq , Albarki Georona Kusma 
Published: January 2025

weet corn productivity in Indonesia is still in the low category. One of the causes is the problem of soil with low pH. Soil with a low pH causes the availability of nutrients to decrease, thereby inhibiting plant growth and development. Efforts can be made to add dolomite, because hydrolyzed dolomite can increase nutrient availability in the soil. Therefore, this research was carried out with the aim of analyzing the effect of adding various doses of dolomite on soil chemical properties, plant uptake of Ca, Mg and P, population of phosphate solubilizing bacteria, and sweet corn productivity in the Tegalan Land of Junrejo Village, Batu City, East Java. The research design used a randomized block design (RAK) consisting of 9 treatments with 3 replications. Treatment consisted of: M0 = control; M1 = 100% N, P, K; M2 = 100% N,P,K + 100% Kieserite; M3 = 100% N,P,K + 50% Dolomit; M4 = 100% N,P,K + 100% Dolomit; M5 = 100% N,P,K + 150% Dolomit; M6 = 75% N,P,K + 50% Dolomit; M7 = 75% N,P,K + 100% Dolomit; M8 = 75% N,P,K + 150% Dolomit. The data was analyzed for diversity using R studio software and continued with the DMRT (Duncan Multiple Range Test) test at 5% level. Next, to see the relationship and influence between parameters, correlation and regression tests were carried out. The findings demonstrated that adding 2.8 tonnes of dolomite per hectare might raise soil CEC (50.61%) and pH (13.41%). In addition, the addition of 4.2 tons/ha of dolomite was able to decrease soil P-available (5.65%), increase Ca-dd (26.4%), Mg-dd (85%), Ca absorption (165.12%), Mg absorption (366.67%), P absorption (78.57%), and sweet corn yield (78.04%). The addition of different doses of dolomite did not significantly alter the overall population parameter of phosphate-solubilizing bacteria. Furthermore, the link between sweet corn yield and nutritional absorption was both robust and weak. The increase in sweet corn output was impacted by P, Ca, and Mg absorption by 54.54%, 41.21%, and 42.79%, respectively.
The Aerated Compost Extract Increases Growth and Productivity of Red Spinach Plants (Amaranthus tricolor L.)
Authors: Siswanti Dwi Umi 
Published: January 2025

Synthetic fertilizers are widely used in agriculture to increase crop yields. However, the impact can suppress soil microbial life and reduce soil quality. Therefore, a study about fertilizer alternative that does not have an environmental bad impact is necessary, and one of which is a study of compost extract. Compost extract is a liquid extract produced from soaking compost for several days by aeration or non-aeration. The aims of the research are to determine the effect and the best dosage of aerated and non-aerated compost extract on plant growth and productivity. The plant that will be used in this research is red spinach (Amaranthus tricolor L.). This reaserch is conducted in a completely randomized design (CRD) with treatment of compost extract dosage. The chemical parameters of the compost extract analyzed included carbon (C), nitrogen (N), phosphorus (P), and potassium (K) levels. Parameters observed are plant height; number of leaves; fresh and dry weight of roots, stems, and leaves; root shoot ratio; and levels of vitamin C. Data is analyzed using Analysis of variance (Anova), and differences between treatments will test with Duncan Multiple Range Test (DMRT) with a significance level of 95%. The aerated compost extract with an optimum concentration of 75% increased plant height, number of leaves, fresh weight of stems and leaves, and dry weight of stems. The aerated compost extract with 100% optimum concentration increased root fresh weight, root and leaf dry weight, root:shoot ratio, and vitamin C.
Keywords: Amaranthus tricolor L.; aerated compost extract; non-aerated compost extract; plant growth; plant productivity.
Research on Nobel Literature Prize winner Yan Mo’s Transformations of Writing Styles from the Perspective of Magical Realism
Authors: Miao Xueyuan 
Published: January 2025

Yan Mo, China’s first Nobel Prize winner for literature, has largely experienced two transformations of writing styles. However, magical realism has always been the main characteristics of his writing creation. During the first period ( between the year 1981 to the time he studied in PLA Academy of Arts) , he concentrated chiefly on his daily life in the army and his writing style was soft and fresh. Then using bold brushstrokes and unique perspectives, he turned to depict the colorful stories of numerous characters in his hometown with magical realism and rich imagination, leading him all the way to his winning of the Nobel Prize for literature in 2012. After experiencing about five years of creative silence, Yan Mo experienced another creative transformation from novels to dramas with the publication of the drama Crocodile as a representative. Dramas are significant components of Yan Mo’s literary works, but most people only attach great importance to Yan Mo’s researches on magical realism in his novels. Fewer people do researches about his dramas, with even fewer researchers making comparison between Yan Mo’s Transformation of Writing styles. So this article is to use the textual analysis to explore the factors that account for his use of magical realism in the two transformations and analyze the inheritances and developments in his writing Characteristics of magical realism in the two stages. In a word, there are four purposes for this paper : (1) to explore the development of magical realism in Yan Mo’s literary creation and the contributing factors. (2) to explore the transformations of magical realism before Yan Mo won the Nobel Prize for literature and its Unique characteristics. (3) to explore the differences of the use of magical Realism after he won the Noble Prize for literature in 2012. (4) to explore the reasons for Yan Mo’s transformation and conclude the research gap.
Pemahaman Kepastian Hukum Hak Atas Tanah pada Aparatur dan Masyarakat Desa Benjor- Kabupaten Malang
Authors: Anadi Yandri Radhi Anadi , Faisol  , Rizki Rizki 
Published: January 2025

Keberadaan kepastian hukum merupakan sebuah bentuk perlindungan bagi pencari keadilan terhadap tindakan sewenang-wenang, yang berarti bahwa seseorang akan dan dapat memperoleh sesuatu yang diharapkan dalam keadaan tertentu secara pasti. pada wilayah Desa Benjor masih terdapat kekurangan pemahaman terhadap sejauh mana kepastian hukum hak atas tanah yang harus diterapkan, baik dilingkup aparatur desa itu sendiri maupun untuk masyarakat. Maka dengan itu diperlukan ekskalasi pemahaman terhadap kepastian hukum hak atas tanah agar tercipatanya tata kelola pemerintahan yang baik dan perwujudan cita-cita untuk keadilan bagi masyrakat melalui pendaftaran tanag yang sudah ditentukan oleh peraturan perundang-undangan. Permasalahan dalam penelitian ini diantaranya intensitas pengetahuan aparatur desa dan masyarakat desa terhadap kepastian hukum hak atas tanah di Desa Benjor serta hambatan aparatur desa dan masyarakat Desa Benjor untuk mendapatkan kepastian hukum terhadap hak atas tanah. Jenis Penelitian yang digunakan adalah penelitia yuridis empiris, dengan menggunakan pendekatan yuridis sosilogis.

Abstract
Understanding of Legal Certainty of Land Rights in the Apparatus and Communities of Benjor Village, Malang Regency.The existence of legal certainty is a form of protection for justice seekers against arbitrary actions, which means that someone will and can get something that is expected under certain circumstances with certainty. in the Benjor Village area there is still a lack of understanding of the extent to which legal certainty of land rights must be applied, both within the village apparatus itself and for the community. So with that it is necessary to escalate understanding of legal certainty of land rights so that good governance is created and the realization of the ideals of justice for the community through land registration which has been determined by laws and regulations. The problems in this study include the intensity of knowledge of village officials and village communities regarding legal certainty of land rights in Benjor Village and obstacles to village apparatus and the people of Benjor Village in obtaining legal certainty regarding land rights. The type of research used is empirical juridical research, using a sociological juridical approach.