bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proposal for the Updating of the ISO 29841 Liquid Chromatographic Method for the Determination of Chlorophyll a and a′ Degradation Products in Virgin Olive Oil
Authors: Kritiktsi Evmorfia , Tsimidou Maria Z. , Nenadis Nikolaos 
Published: January 2022

AbstractPyropheophytin a (PPPa) found in minute amounts in virgin olive oil under good manufacturing practice conditions and increasing as a function of storage temperature and length, has attracted the interest of scientists as a marker of quality. Certain bodies developing olive oil standards have adopted its content (% PPPamax < 17 throughout the shelf‐life of a product) as a quality criterion. The analytical protocol to examine product compliance is the ISO 29841 method. In the present work, green improvements are proposed through the use of a short column and vacuum in the solid‐phase sample extraction step. The preparation of handmade cartridges that can be reused without pretreatment at least six times reduces further the cost of analysis. The proposed protocol is repeatable and robust. In case fluorescence detection is used, selection of λex = 407 nm/λem = 672 nm instead of λex = 430 nm/λem = 670 nm increases detection sensitivity of the target compounds by 1.5‐ to 2.6‐fold. Fluorescence detection results in higher %PPPa values by 2% on average compared to those obtained with a diode array detector set at 410 nm. Present proposals and observations are expected to be of interest for the next edition of the ISO 29841:2009/AMD1:2016.Practical applications: The proposals made in the present study expedite significantly the analysis, thus, increasing the sample throughput even compared to other alternative fast approaches in the literature. The reduction of the use of chemicals adds to the modification of the ISO 29841 method to an analytical tool that abides by green and sustainable chemistry which is considered critical for “beyond the 2020 process.” The proposed chromatographic column updates the method as its effective use in an HPLC instrument provides comparable efficiency to a UHPLC one, and makes method transfer to the latter without adjustments. In this way, method adoption may be wider by analytical laboratories involved in virgin olive oil quality control. Finally, the identification of quantitative differences using different detection means (visible, fluorescence) for the first time, when addressed by the ISO committee, may assist the effective quality control and facilitate the virgin olive oil international trade.
Synthesis of 3‐Acyl‐Isoxazoles via Radical 5‐endo trig Cyclization of β,γ‐Unsaturated Ketones with NaNO2
Authors: Jiang Ping , Wang Yaming , Chen Dengfeng , Zheng Yu , Huang Shenlin 
Published: January 2022

AbstractHere we report a facile cyclization reaction of β,γ‐unsaturated ketones with NaNO2 under mild conditions to construct 3‐acyl‐isoxazoles. This transformation is realized via nitrosation of the activated methylene, radical 5‐endo trig cyclization, and then aromatization, according to some control experiments. Further, this protocol provided an efficient access to a variety of isoxazoles from readily available allyl ketones.
Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells
Authors: Verbelen Benno , Girardi Tiziana , Sulima Sergey O. , Vereecke Stijn , Verstraete Paulien , Verbeeck Jelle , Royaert Jonathan , Cinque Sonia , Montanaro Lorenzo , Penzo Marianna , Imbrechts Maya , Geukens Nick , Geuens Thomas , Dierckx Koen , Pepe Daniele , Kampen Kim , De Keersmaecker Kim 
Published: January 2022

AbstractMammalian cells are commonly used to produce recombinant protein therapeutics, but suffer from a high cost per mg of protein produced. There is therefore great interest in improving protein yields to reduce production cost. We present an entirely novel approach to reach this goal through direct engineering of the cellular translation machinery by introducing the R98S point mutation in the catalytically essential ribosomal protein L10 (RPL10‐R98S). Our data support that RPL10‐R98S enhances translation levels and fidelity and reduces proteasomal activity in lymphoid Ba/F3 and Jurkat cell models. In HEK293T cells cultured in chemically defined medium, knock‐in of RPL10‐R98S was associated with a 1.7‐ to 2.5‐fold increased production of four transiently expressed recombinant proteins and 1.7‐fold for one out of two stably expressed proteins. In CHO‐S cells, eGFP reached a 2‐fold increased expression under stable but not transient conditions, but there was no production benefit for monoclonal antibodies. The RPL10‐R98S associated production gain thus depends on culture conditions, cell type, and the nature of the expressed protein. Our study demonstrates the potential for using a ribosomal protein mutation for pharmaceutical protein production gains, and further research on how various factors influence RPL10‐R98S phenotypes can maximize its exploitability for the mammalian protein production industry.
Seven decades of shoreline changes along a muddy mangrove coastline of the Upper Gulf of Thailand
Authors: Sok Kimhuy , Bidorn Butsawan , Burnett William C. , Sasaki Jun , Sola Phachirarat 
Published: January 2022

AbstractThis study evaluates the historical coastal change and development along the eastern coastline of the Upper Gulf of Thailand between 1953 and 2019 based on a series of aerial photographs and satellite images. Long‐term (~70 years) and short‐term (~10 years) shoreline movement rates were analysed using the Digital Shoreline Analysis System (DSAS). Results of this analysis indicate that the shoreline along the western coast (WBK) of the Bang Pakong River mouth has undergone severe coastal degradation, with a land loss of about 980 ha over the last seven decades. Meanwhile, the eastern coastline (EBK) has developed continuously over this time span, resulting in a land growth of about 552 ha. Based on historical shorelines recorded since 1954, the shoreline recession along the WBK coast accelerated over five decades. The average shoreline change rate reached approximately −9 m/year in 2002 and is shown to be related to land subsidence, which was due to groundwater withdrawals. The WBK coastline stabilized in 2009 and became an accretionary coast after 2016 due to the introduction of engineered coastal protection measures. In addition, the construction of major dams on the Chao Phraya and Bang Pakong rivers did not result in severe shoreline recession along the WBK coast. A significant reduction in sediment supply to the coastal zone due to damming was not observed. In contrast, the EBK coastline has advanced continuously since 1953, with average growth rates between 0.5 and 7.6 m/year over 50 years as a result of increased riverine sediment supply. Our results also indicate that mangrove deforestation was not a major factor causing shoreline retreat, and mangrove reforestation was not a successful coastal protection approach in this coast.
Nurturing the marriages of single atoms with atomic clusters and nanoparticles for better heterogeneous electrocatalysis
Authors: Zhang Lei , Zhu Jiawei , Li Xin , Mu Shichun , Verpoort Francis , Xue Junmin , Kou Zongkui , Wang John 
Published: January 2022

AbstractSingle‐atom catalysts, featuring some of the most unique activities, selectivity, and high metal utilization, have been extensively studied over the past decade. Given their high activity, selectivity, especially towards small molecules or key intermediate conversions, they can be synergized together with other active species (typically other single atoms, atomic clusters, or nanoparticles) in either tandem or parallel or both, leading to much better performance in complex catalytic processes. Although there have been reports on effectively combining the multiple components into one single catalytic entity, the combination and synergy between single atoms and other active species have not been reviewed and examined in a systematic manner. Herein, in this overview, the key synergistic interactions, binary complementary effects, and the bifunctional functions of single atoms with other active species are defined and discussed in detail. The integration functions of their marriages are investigated with particular emphasis on the homogeneous and heterogeneous combinations, spatial distribution, synthetic strategies, and the thus‐derived outstanding catalytic performance, together with new light shined on the catalytic mechanisms by zooming in several case studies. The dynamic nature of each of the active species and in particular their interactions in such new catalytic entities in the heterogeneous electrocatalytic processes are visited, on the basis of the in situ/operando evidence. Last, we feature the current challenges and future perspectives of these integrated catalytic entities that can offer guidance for advanced catalyst design by the rational combination and synergy of binary or multiple active species.
Curcumin attenuates isoniazid‐induced hepatotoxicity by upregulating the SIRT1/PGC‐1α/NRF1 pathway
Authors: Li Yun , Luo Wen‐wen , Cheng Xuan , Xiang Huai‐rong , He Bei , Zhang Qi‐zhi , Peng Wen‐xing 
Published: January 2022

AbstractAs a serious infectious disease, tuberculosis threatens global public health. Isoniazid is the first‐line drug not only in active tuberculosis but also in its prevention. Severe hepatotoxicity greatly limits its use. Curcumin, extracted from turmeric, has been found to relieve isoniazid‐induced hepatotoxicity. However, the mechanism of isoniazid‐induced hepatotoxicity and the protective effects of curcumin are not yet understood completely. We established both cell and animal models about isoniazid‐induced hepatotoxicity and investigated the new mechanism of curcumin against isoniazid‐induced liver injury. The experimental data in our study demonstrated that curcumin ameliorated isoniazid‐mediated liver oxidative stress. The protective effects of curcumin were demonstrated and confirmed to be correlated with upregulating SIRT1/PGC‐1α/NRF1 pathway. Western blot revealed that while inhibiting SIRT1 by the siRNA1 (a SIRT1 inhibitor), the expressions of SIRT1, PGC‐1α/Ac‐PGC‐1α, and NRF1 decreased, and the protective effect that curcumin exerted on isoniazid‐treated L‐02 cells was significantly attenuated. Furthermore, curcumin improved liver functions and reduced necrosis of the isoniazid‐treated BALB/c mice, accompanied by downregulating oxidative stress and inflammation in liver. Western blot revealed that curcumin treatment activates the SIRT1/PGC‐1α/NRF1 pathway in the isoniazid‐treated BALB/c mice. In conclusion, we found one mechanism of isoniazid‐induced hepatotoxicity downregulating the SIRT1/PGC‐1α/NRF1 pathway, and curcumin attenuated this hepatotoxicity by activating it. Our study provided a novel approach and mechanism for the treatment of isoniazid‐induced hepatotoxicity.
Using meditation in a control vs. acceptance context: A preliminary evaluation of relations with anxiety, depression, and indices of well‐being
Authors: Tifft Eric D. , Underwood Shannon B. , Roberts Max Z. , Forsyth John P. 
Published: January 2022

AbstractBackground and ObjectivesMeditation practices have been marketed broadly to ameliorate human suffering. As such, individuals may seek out and use meditation to control or manage unpleasant thoughts and emotions. Emotion and thought control research suggest that meditation used in this way may potentiate unpleasant private experiences and contribute to negative outcomes. We aimed to evaluate the function or purpose guiding meditation and its relations with anxiety, depression, and other indices of well‐being.Design and MethodsIn a cross‐sectional design, undergraduate meditators (N = 98) reported intentions guiding their meditation practice (i.e., experiential/emotional control or acceptance/openness) and completed an assessment battery.ResultsMost participants (58.2%) indicated using meditation to manage, control, or avoid difficult experiences. Participants using meditation with control‐based intentions reported greater worry, anxiety, depression, negative affect, and lower mindfulness relative to their acceptance‐guided counterparts. After controlling for level of anxiety, viewing anxiety as a problem increased the likelihood of using meditation with control‐based intentions. Similar relations were observed between viewing stress as a problem and the likelihood of using meditation for experiential control.ConclusionsFindings suggest that (a) how people meditate is significantly related to psychological distress and (b) highlight the importance of evaluating intentions guiding meditative practices, particularly in individuals struggling with unpleasant emotional or psychological experiences.
What relates to length of stay in a sober living home among women with histories of victimization and addiction? A longitudinal analysis
Authors: Edwards Katie M. , Ullman Sarah E. , Siller Laura , Murphy Sharon B. , Harvey Ronald , Wheeler Lorey 
Published: January 2022

AbstractDespite preliminary research suggesting that length of stay in sober living homes (SLHs) is related to sustained sobriety, little research has examined factors that relate to length of stay in SLHs. The purpose of the proposed exploratory study was to prospectively examine baseline characteristics of women with histories of addiction and victimization as correlates of length of stay in a trauma‐informed, gender‐responsive SLH. Participants (N = 45) were surveyed three times over a 1‐year period. Women were invited to participate within a week of their arrival to the SLH. Nearly two‐thirds (62.2%, n = 28) of women stayed under 3 months, and 37.8% (n = 17) of women stayed over 3 months. Whereas older age and greater financial worries were associated with staying over 3 months at the SLH, other variables (e.g., demographics, mental health, recent victimization, recent substance use) were unrelated. Findings indicate that efforts may be needed to ensure that younger women as well as women with less financial worries, who may be less likely to stay for longer periods of times at SLHs, have adequate support for sobriety.
Generating youth dialogue through the literary arts: A citywide youth health collaboration in the U.S.
Authors: Golden Tasha L. , Sima Richard , Roebuck Grace , Gupta Sonakshi , Magsamen Susan 
Published: January 2022

AbstractA process and outcomes evaluation was conducted of a citywide literary‐arts initiative designed to reduce stigma, amplify underrepresented narratives, and generate dialogue about violence. Over 4 months, students in 85 middle schools read a novel addressing mental health and violence‐related themes. As a collaboration between a public school district and public library system, the program's classroom activities emphasized dialogue, while coordinated library events supported community engagement. Students completed pre/postsurveys (total n = 1487); school program leaders (n = 39) and public librarians (n = 14) completed postsurveys. Half of student respondents reported personal encounters with violence. Most said the novel influenced their thinking; students with personal experiences of violence reported greater influence than those without. Highest rates of dialogue were seen among students who read the novel in full, and over half of postsurvey respondents wanted more dialogue opportunities. Leaders and librarians indicated that the program was smoothly integrated with existing curricula, but also offered recommendations for improved processes. Findings suggest that this intervention was effective at stimulating dialogue while providing assets‐based opportunities for youth to share experiences and concerns. Current program processes indicate promise for further engagement and impact, with some modifications. To this end, authors draw upon evaluation findings and youth development literature to offer recommendations for program development and replication in other regions.
Cascade testing for inherited arrhythmia conditions: Experiences and attitudes of family communication approaches for a Canadian cohort
Authors: Ho Amy , Leach Emma , Virani Alice , Arbour Laura , Bartels Kirsten , Wong Eugene K. 
Published: January 2022

AbstractInherited arrhythmia conditions (IAC) can lead to sudden cardiac death at any age, and relatives of an affected person have up to a 50% chance of inheriting the condition and are at risk for developing features. Cascade testing is a stepwise approach for identifying relatives at risk for IACs through clinical screening and genetic testing. Early detection can reduce morbidity and mortality for affected individuals and determine potential risk mitigation strategies for relatives. However, cardiovascular genetic studies have reported an incomplete uptake of cascade testing in at‐risk relatives. We explored patient perspectives on cascade testing for IACs and alternative approaches to family communication. Twelve semi‐structured phone interviews were conducted with probands of the British Columbia Inherited Arrhythmia Program confirmed to carry a pathogenic or likely pathogenic variant in a gene associated with an IAC. Thematic analysis of transcripts through an iterative coding process revealed five main themes: (a) a stepwise approach is followed in disclosing risk to relatives, (b) relatives’ autonomy in cascade testing is supported, (c) lived experience with the condition influences disclosure and uptake of cascade testing, (d) collaborative approach to informing relatives reduces negative impact of disclosure, and (e) direct contact from a healthcare provider is viewed as acceptable. The findings highlight this patient cohort's experiences and opinions with approaches to disclosure and demonstrate their understanding and acceptance of their relatives’ approaches to cascade testing. In addition, while the notion of direct contact was generally accepted, a collaborative approach to contacting relatives between the proband and provider may be most effective.
lncRNA MALAT1 promotes osteogenic differentiation through the miR‐217/AKT3 axis: A possible strategy to alleviate osteoporosis
Authors: Song Chenyang , Guo Yu , Chen Fenyong , Liu Wenge 
Published: January 2022

AbstractBackgroundAccumulating evidence demonstrates that long non‐coding RNAs (lncRNAs) are associated with the development of osteoporosis. The present study aimed to investigate the effect of metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) on osteogenic differentiation in osteoporosis.MethodsMALAT1 levels were detected by a real‐time polymerase chain reaction (RT‐qPCR). Moreover, the levels of osteogenic differentiation‐related factors (Bmp4, Col1a1 and Spp1) were measured by a RT‐qPCR and western blotting. Alkaline phosphatase (ALP) activity was detected using an ALP staining assay.ResultsMALAT1 levels were downregulated in hindlimb unloading mice and simulated in microgravity (MG) treated MC3T3‐E1 cells. Moreover, MG treatment induced the downregulation of the expression of ALP, BMP4, Col1a1 and Spp1, whereas overexpression of MALAT1 abolished the downregulation. MG also inhibited ALP activity, whereas MALAT1 reversed the effect. Furthermore, miR‐217 was identified as a target of MALAT1, and AKT3 was verified as a target of miR‐217. Overexpression of miR‐217 rescued the promotion of osteogenic differentiation induced by MALAT1 in MG treated cells. Knockdown of AKT3 abolished the facilitation of osteogenic differentiation induced by downregulation of miR‐217.ConclusionsMALAT1 promotes osteogenic differentiation through regulating miR‐217/AKT3 axis, suggesting that MALAT1 is a potential target with respect to alleviating osteoporosis.
Brain and visceral gene editing of mucopolysaccharidosis I mice by nasal delivery of the CRISPR/Cas9 system
Authors: Vera Luisa Natalia Pimentel , Schuh Roselena Silvestri , Fachel Flavia Nathielly Silveira , Poletto Edina , Piovesan Eduarda , Kubaski Francyne , Couto Eduarda , Brum Bruna , Rodrigues Graziella , Souza Hallana , Giugliani Roberto , Matte Ursula , Baldo Guilherme , Teixeira Helder F. 
Published: January 2022

AbstractBackgroundMucopolysaccharidosis type I (MPS I) is an inherited disease caused by deficiency of the enzyme alpha‐l‐iduronidase (IDUA). MPS I affects several tissues, including the brain, leading to cognitive impairment in the severe form of the disease. Currently available treatments do not reach the brain. Therefore, in this study, we performed nasal administration (NA) of liposomal complexes carrying two plasmids encoding for the CRISPR/Cas9 system and for the IDUA gene targeting the ROSA26 locus, aiming at brain delivery in MPS I mice.MethodsLiposomes were prepared by microfluidization, and the plasmids were complexed to the formulations by adsorption. Physicochemical characterization of the formulations and complexes, in vitro permeation, and mucoadhesion in porcine nasal mucosa (PNM) were assessed. We performed NA repeatedly for 30 days in young MPS I mice, which were euthanized at 6 months of age after performing behavioral tasks, and biochemical and molecular aspects were evaluated.ResultsMonodisperse mucoadhesive complexes around 110 nm, which are able to efficiently permeate the PNM. In animals, the treatment led to a modest increase in IDUA activity in the lung, heart, and brain areas, with reduction of glycosaminoglycan (GAG) levels in serum, urine, tissues, and brain cortex. Furthermore, treated mice showed improvement in behavioral tests, suggesting prevention of the cognitive damage.ConclusionNonviral gene editing performed through nasal route represents a potential therapeutic alternative for the somatic and neurologic symptoms of MPS I and possibly for other neurological disorders.
Assessment of CD39 expression in regulatory T‐cell subsets by disease severity in adult and juvenile COVID‐19 cases
Authors: Simsek Abdurrahman , Kizmaz Muhammed A. , Cagan Eren , Dombaz Fatma , Tezcan Gulcin , Asan Ali , Demir H. Ibrahim , Bal S. Haldun , Ermis Digdem Y. , Dilektaslı Aslı G. , Kazak Esra , Akalin E. Halis , Oral H. Barbaros , Budak Ferah 
Published: January 2022

AbstractCOVID‐19 is a disease characterized by acute respiratory failure and is a major health problem worldwide. Here, we aimed to investigate the role of CD39 expression in Treg cell subsets in COVID‐19 immunopathogenesis and its relationship to disease severity. One hundred and ninety COVID‐19 patients (juveniles, adults) and 43 volunteers as healthy controls were enrolled in our study. Flow cytometric analysis was performed using a 10‐color monoclonal antibody panel from peripheral blood samples. In adult patients, CD39+ Tregs increased with disease severity. In contrast, CD39+ Tregs were decreased in juvenile patients in an age‐dependent manner. Overall, our study reveals an interesting profile of CD39‐expressing Tregs in adult and juvenile cases of COVID‐19. Our results provide a better understanding of the possible role of Tregs in the mechanism of immune response in COVID‐19 cases.