bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Personal Values in Adolescence with Subjective Health Status, Meaning in Life, and Life Satisfaction in Adulthood: A Cross‐sectional Study with Retrospective Recall1
Authors: Iida Mako , Watanabe Kazuhiro , Yeo Seon Ae , Yasuma Naonori , Nishi Daisuke , Kawakami Norito 
Published: November 2022

AbstractThis study aimed to replicate a previous finding of the association between personal values in adolescence and health/mental well‐being in adulthood among workers, in a large community sample, with an additional focus on eudemonic well‐being. A cross‐sectional study was conducted in urban and suburban cities of Japan. Subjective health status, eudemonic well‐being (meaning in life), and life satisfaction in adulthood were measured in 2010; 11 types of personal values and commitment to values in adolescence were assessed with the retrospective recall in 2017. A total of 2,413 adults were included in the multiple linear regression analyses. None of the personal values were related to life satisfaction unlike the previous study. Consistent results were that commitment to values was positively associated with life satisfaction, and the value of graduating from school was positively associated with subjective health status. Personal values related to openness to change and self‐enhancement were positively associated with meaning in life in adulthood. This study did not fully replicate previous findings, but added the eudemonic perspective of mental well‐being to the previous study.
Effect of osteoarthritis on alveolar bone loss in experimental periodontitis in rats
Authors: Al Bayaty Fouad H. , Mahmod Suhaeb A. , Jamil Al‐Obaidi Mazen M. , Emad Ibrahim Omar , Dahir Akil , Adam Fara Azwin , Albandar Jasim M. 
Published: November 2022

AbstractBackgroundThere is scarce information about the relationship between periodontal disease and osteoarthritis. This study investigated the effect of surgically induced osteoarthritis on alveolar bone loss in experimental periodontitis in rats.Methods12 rats were divided into test and control groups. On day 1, the animals were anaesthetized, and silk ligatures were ligated around 6 maxillary posterior teeth in each animal from both groups. Surgical induction of osteoarthritis was performed on the left knees in the test group. No knee surgeries were performed in the control group. The ligatures were kept in place for 30 days, at which time the animals were euthanatized, and the maxillae and knee joints were harvested and processed for histological analysis. The alveolar bone loss was assessed using a zoom stereomicroscope.ResultsThe knee joint histologic sections of the control group showed normal joint features, whereas in the test group there were substantial changes typical of osteoarthritis, including wide joint spaces, prominent monocytic infiltration of the synovium, invasion of periarticular bone, and decreased chondrocyte density. Comparison of the bone height between the groups showed a significantly higher bone loss in the test than in the control group The marginal mean bone height, adjusted for covariates and the intraclass correlation between sites, was 1.19 and 0.78 mm in the test and control groups, respectively (p < .0001).ConclusionsSurgically induced osteoarthritis leads to greater alveolar bone loss in the experimental periodontitis model in rats.
An observatory on changes in dreaming during a pandemic: a living systematic review (part 1)
Authors: Margherita Giorgia , Caffieri Alessia 
Published: November 2022

SummaryA ‘new’ way of dreaming has emerged during the pandemic, enhancing the interest of psychological literature. Indeed, during the years of the spread of coronavirus disease 2019 (COVID‐19), many studies have investigated dream‐related phenomena and dreaming functions. Considering the constant and rapid emergence of new results on this topic, the main aim of this study was to create an ‘observatory’ on the short‐ and long‐term consequences of the COVID‐19 pandemic on dreaming, by means of a living systematic review. The baseline results are presented, in accordance with the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses statement guidelines, to identify and discuss existing studies about dreams and dreaming during the COVID‐19 pandemic published until February 2022. Web of Science, Embase, EBSCO, and PubMed were used for the search strategy, yielding 71 eligible papers included in the review. Our results show: (a) a more intense oneiric activity during lockdown; (b) changes in dreaming components (especially dream‐recall and nightmare frequency); (c) a particular dreaming scenario (‘pandemic dreams’); (d) an alteration of the dreaming–waking‐life continuum and a specific function of dreaming as emotional regulator. Findings suggest that monitoring changes in dreaming provides important information about psychological health and could also contribute to the debate on the difficulties of dreaming, as well as sleeping, in particular during and after a period of ‘collective trauma’.
Molecular phenotyping uncovers differences in basic housekeeping functions among closely related species of hares (Lepus spp., Lagomorpha: Leporidae)
Authors: Gaertner Kateryna , Michell Craig , Tapanainen Riikka , Goffart Steffi , Saari Sina , Soininmäki Manu , Dufour Eric , Pohjoismäki Jaakko L. O. 
Published: November 2022

AbstractSpeciation is a fundamental evolutionary process, which results in genetic differentiation of populations and manifests as discrete morphological, physiological and behavioural differences. Each species has travelled its own evolutionary trajectory, influenced by random drift and driven by various types of natural selection, making the association of genetic differences between the species with the phenotypic differences extremely complex to dissect. In the present study, we have used an in vitro model to analyse in depth the genetic and gene regulation differences between fibroblasts of two closely related mammals, the arctic/subarctic mountain hare (Lepus timidus Linnaeus) and the temperate steppe‐climate adapted brown hare (Lepus europaeus Pallas). We discovered the existence of a species‐specific expression pattern of 1623 genes, manifesting in differences in cell growth, cell cycle control, respiration, and metabolism. Interspecific differences in the housekeeping functions of fibroblast cells suggest that speciation acts on fundamental cellular processes, even in these two interfertile species. Our results help to understand the molecular constituents of a species difference on a cellular level, which could contribute to the maintenance of the species boundary.
Transcriptome‐based analyses of adaptive divergence between two closely related spruce species on the Qinghai–Tibet plateau and adjacent regions
Authors: Feng Shuo , Wan Wei , Li Yang , Wang DongLei , Ren Guangpeng , Ma Tao , Ru Dafu 
Published: November 2022

AbstractSpeciation among populations connected by gene flow is driven by adaptation to different environments, but underlying gene–environment associations remain largely unknown. Here, 162 individuals from 32 populations were sampled to obtain 191,648 independent single nucleotide polymorphisms (SNPs) across the genomes of two closely related spruce species, Picea asperata and Picea crassifolia, which occur on the Qinghai–Tibet Plateau and in surrounding regions. Using the SNP data set, genotype–environment associations and demographic modelling were used to examine local adaptation and genetic divergence between these two species. While morphologically similar, the two Picea species were genetically differentiated in multiple analyses. These species diverged despite continuous gene flow, and their initial divergence was dated back to the late Quaternary. The effective population sizes of both species have expanded since their divergence, as confirmed by niche distribution simulations. A total of 6365 genes were associated with the tested environmental variables; of these, 41 were positively selected in P. asperata and were mainly associated with temperature, while 83 were positively selected in P. crassifolia and were primarily associated with precipitation. These results deepen our understanding of the adaptive divergence and demographic histories of these two spruce species and highlight the importance of genomic data in deciphering the environmental selection underlying Quaternary interspecific divergence.
How ancient forest fragmentation and riparian connectivity generate high levels of genetic diversity in a microendemic Malagasy tree
Authors: Salmona Jordi , Dresen Axel , Ranaivoson Anicet E. , Manzi Sophie , Le Pors Barbara , Hong‐Wa Cynthia , Razanatsoa Jacqueline , Andriaholinirina Nicole V. , Rasoloharijaona Solofonirina , Vavitsara Marie‐Elodie , Besnard Guillaume 
Published: November 2022

AbstractUnderstanding landscape changes is central to predicting evolutionary trajectories and defining conservation practices. While human‐driven deforestation is intense throughout Madagascar, exceptions in areas such as the Loky‐Manambato region (north) raise questions regarding the causes and age of forest fragmentation. The Loky‐Manambato region also harbours a rich and endemic flora, whose evolutionary origin remains poorly understood. We assessed the genetic diversity of an endangered microendemic Malagasy olive species (Noronhia spinifolia Hong‐Wa) to better understand the vegetation dynamics in the Loky‐Manambato region and its influence on past evolutionary processes. We characterized 72 individuals sampled across eight forests through nuclear and mitochondrial restriction‐associated DNA sequencing data and chloroplast microsatellites. Combined population and landscape genetics analyses indicate that N. spinifolia diversity is largely explained by the current forest cover, highlighting a long‐standing habitat mosaic in the region. This sustains a major and long‐term role of riparian corridors in maintaining connectivity across these antique mosaic habitats, calling for the study of organismal interactions that promote gene flow.
OXIDATIVE SIGNAL‐INDUCIBLE1 induces immunity by coordinating N‐hydroxypipecolic acid, salicylic acid, and camalexin synthesis
Authors: Rawat Anamika A. , Hartmann Michael , Harzen Anne , Lugan Raphael , Stolze Sara Christina , Forzani Celine , Abts Laura , Reißenweber Sophie , Rayapuram Naganand , Nakagami Hirofumi , Zeier Jürgen , Hirt Heribert 
Published: November 2022

Summary

Expression of OXIDATIVE SIGNAL‐INDUCIBLE1 (OXI1) is induced by a number of stress conditions and regulates the interaction of plants with pathogenic and beneficial microbes.

In this work, we generated Arabidopsis OXI1 knockout and genomic OXI1 overexpression lines and show by transcriptome, proteome, and metabolome analysis that OXI1 triggers ALD1, SARD4, and FMO1 expressions to promote the biosynthesis of pipecolic acid (Pip) and N‐hydroxypipecolic acid (NHP).

OXI1 contributes to enhanced immunity by induced SA biosynthesis via CBP60g‐induced expression of SID2 and camalexin accumulation via WRKY33‐targeted transcription of PAD3. OXI1 regulates genes involved in reactive oxygen species (ROS) generation such as RbohD and RbohF. OXI1 knock out plants show enhanced expression of nuclear and chloroplast genes of photosynthesis and enhanced growth under ambient conditions, while OXI1 overexpressing plants accumulate NHP, SA, camalexin, and ROS and show a gain‐of‐function (GOF) cell death phenotype and enhanced pathogen resistance. The OXI1 GOF phenotypes are completely suppressed when compromising N‐hydroxypipecolic acid (NHP) synthesis in the fmo1 or ald1 background, showing that OXI1 regulation of immunity is mediated via the NHP pathway.

Overall, these results show that OXI1 plays a key role in basal and effector‐triggered plant immunity by regulating defense and programmed cell death via biosynthesis of salicylic acid, N‐hydroxypipecolic acid, and camalexin.
The effect of the severity of neurocognitive disorders on emotional and motor responses to music
Authors: Hobeika Lise , Ghilain Matthieu , Schiaratura Loris , Lesaffre Micheline , Puisieux François , Huvent‐Grelle Dominique , Samson Séverine 
Published: November 2022

AbstractThe successful design of musical interventions for dementia patients requires knowledge of how rhythmic abilities change with disease severity. In this study, we tested the impact of the severity of the neurocognitive disorders (NCD) on the socioemotional and motor responses to music in three groups of patients with Major NCD, Mild NCD, or No NCD. Patients were asked to tap to a metronomic or musical rhythm while facing a live musician or through a video. We recorded their emotional facial reactions and their sensorimotor synchronization (SMS) abilities. Patients with No NCD or Mild NCD expressed positive socioemotional reactions to music, but patients with Major NCD did not, indicating a decrease in the positive emotional impact of music at this stage of the disease. SMS to a metronome was less regular and less precise in patients with a Major NCD than in patients with No NCD or Mild NCD, which was not the case when tapping with music, particularly in the presence of a live musician, suggesting the relevance of live performance for patients with Major NCD. These findings suggest that the socioemotional and motor reactions to music are negatively affected by the progression of the NCD.
CXCL8, MMP12, and MMP13 are common biomarkers of periodontitis and oral squamous cell carcinoma
Authors: Chen Xin , Lei Hao , Cheng Yuxun , Fang Shishu , Sun Weifu , Zhang Xiaochen , Jin Zuolin 
Published: November 2022

AbstractObjectiveTo analysis the relationship between periodontitis (PD) and oral squamous cell carcinoma (OSCC) by bioinformatic analysis.Materials and MethodsWe analyzed the gene expression profiles of PD (GSE16134) from the Gene Expression Omnibus (GEO) database and OSCC samples from TCGA‐HNSC (head and neck squamous cell carcinoma) and identified common differentially expressed genes (DEGs) in PD and OSCC. Then, functional annotation and signaling pathway enrichment, protein interaction network construction, and hub gene identification were performed. Subsequently, the function and signaling pathway enrichment of hub genes, miRNA interaction, and transcription factor interaction analyses were carried out. We analyzed GSE10334 and GSE30784 as validation datasets, and performed qRT‐PCR experiments simultaneously for validation, and obtained 4 hub genes. Finally, immune infiltration analysis and clinical correlation analysis of 4 hub genes and related miRNAs were performed.ResultsWe identified 31 DEGs (16 up‐regulated and 15 down‐regulated). Four hub genes were obtained by qRT‐PCR and validation dataset analysis, including IL‐1β, CXCL8, MMP12, and MMP13. The expression levels of them were all significantly upregulated in both diseases. The functions of these genes focus on three areas: neutrophil chemotaxis, migration, and CXCR chemokine receptor binding. Key pathways include IL‐17 signaling pathway, chemokine signaling pathway, and cytokine–cytokine receptor interactions pathway. Immune infiltration analysis showed that the expressions of 4 hub genes were closely related to a variety of immune cells. ROC curve analysis indicated that AUCs of 4 hub genes are all greater than 0.7, among which MMP12 and MMP13 were greater than 0.9. Kaplan–Meier survival analysis indicated that worse OS was strongly correlated with CXCL8 and MMP13 high‐expression groups. MMP12 low‐expression group was strongly associated with worse OS. The results of multivariate Cox regression analysis showed that age, N stage, CXCL8, MMP12, and MMP13 were independent prognostic factors for OS. We also identified 3 miRNAs, including hsa‐miR‐19b‐3p, hsa‐miR‐181b‐2‐3p, and hsa‐miR‐495‐3p, that were closely related to 4 hub genes. Hsa‐miR‐495‐3p is closely related to the diagnosis and prognosis of OSCC.ConclusionsWe identified 4 hub genes between PD and OSCC, including IL‐1β, CXCL8, MMP12, and MMP13. These genes may mediate the co‐morbid process of PD and OSCC through inflammation‐related pathways such as the IL‐17 signaling pathway. It is worth noting that CXCL8, MMP12, and MMP13 have great significance in the diagnosis and prognosis of OSCC.
Association between oral infections, triglyceride‐glucose index, and in‐stent restenosis
Authors: Wu Yingle , Du Laijing , Fan Mengnan , Chen Xinzhao , Tang Yanyan , Wang Yanyu , Wang Ke , Wang Shaoxin , Li Guangping 
Published: November 2022

AbstractObjectivesTo investigate oral infections in patients suffering in‐stent restenosis (ISR) and non‐ISR and analyze the possible correlation between the oral infection and triglyceride‐glucose (TyG) index, a clinical surrogate indicator of insulin resistance (IR).Materials and MethodsA cross‐sectional design was used, in which 586 patients with acute coronary syndrome who underwent coronary angiography 6–24 months after coronary stent implantation were recruited. The modified total dental index (TDI) was used to evaluate the status of oral inflammation.ResultsIn both univariate analyses, TDI scores [3 (1.5, 4.5) vs. 2.5 (1.5, 4.0), p < 0.01] and a multivariate regression model (OR = 1.202, 95% CI = 1.085–1.333, p < 0.01), the TDI significantly correlated with ISR. The TyG index was positively associated with ISR (OR = 1.766, 95% CI = 1.055–2.957, p < 0.05). Correlation analysis showed that TDI was positively correlated with TyG index (r = 0.190, p < 0.01). Using linear regression analysis, higher TDI scores were significantly associated with IR (95% CI = 0.029–0.063, p < 0.01).ConclusionsOral infections and TyG index were independently and positively correlated with ISR in patients with acute coronary syndrome. Oral inflammatory burden assessed by TDI score was associated with IR.
Amoxicillin vs third‐generation cephalosporin for infection prophylaxis after third molar extraction
Authors: Sato Misuzu , Yamana Hayato , Ono Sachiko , Ishimaru Miho , Matsui Hiroki , Yasunaga Hideo 
Published: November 2022

AbstractObjectivesAlthough amoxicillin is the first‐line prophylactic drug for impacted mandibular third molar extraction, third‐generation cephalosporins are widely prescribed in Japan. The lack of real‐world evidence may be one reason for this inappropriate use. We evaluated differences in the incidence of surgical site infection between amoxicillin and third‐generation cephalosporins for impacted mandibular third molar extraction.MethodsUsing the JMDC Claims Database, we identified dental visits with fully or horizontally impacted mandibular third molar extraction from April 2015 to March 2020. One‐to‐one propensity‐score matching was conducted between amoxicillin and third‐generation cephalosporin groups. The incidence of surgical site infection following extraction was compared in the matched pairs using McNemar's test.ResultsWe identified 109,266 dental visits, including 39,514 (36.2%) patients who received amoxicillin and 69,752 (63.8%) patients who received third‐generation cephalosporins. In the 39,514 matched pairs, the incidence of surgical site infection was 3.5% (n = 1399) for amoxicillin group and 3.7% (n = 1467) for third‐generation cephalosporin group (p = 0.003).ConclusionsAmoxicillin was associated with a lower incidence of surgical site infection after impacted mandibular third molar extraction compared with third‐generation cephalosporins. This result supports current guidelines and strengthens the importance of disseminating and implementing antimicrobial resistance control in dentistry.
The lifetime of the oxygen‐evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas
Authors: Vidal‐Meireles André , Kuntam Soujanya , Széles Eszter , Tóth Dávid , Neupert Juliane , Bock Ralph , Tóth Szilvia Z. 
Published: November 2022

AbstractPSBO is essential for the assembly of the oxygen‐evolving complex in plants and green algae. Despite its importance, we lack essential information on its lifetime and how it depends on the environmental conditions. We have generated nitrate‐inducible PSBO amiRNA lines in the green alga Chlamydomonas reinhardtii. Transgenic strains grew normally under non‐inducing conditions, and their photosynthetic performance was comparable to the control strain. Upon induction of the PSBO amiRNA constructs, cell division halted. In acetate‐containing medium, cellular PSBO protein levels decreased by 60% within 24 h in the dark, by 75% in moderate light, and in high light, the protein completely degraded. Consequently, the photosynthetic apparatus became strongly damaged, probably due to ‘donor‐side‐induced photoinhibition’, and cellular ultrastructure was also severely affected. However, in the absence of acetate during induction, PSBO was remarkably stable at all light intensities and less substantial changes occurred in photosynthesis. Our results demonstrate that the lifetime of PSBO strongly depends on the light intensity and carbon availability, and thus, on the metabolic status of the cells. We also confirm that PSBO is required for photosystem II stability in C. reinhardtii and demonstrate that its specific loss also entails substantial changes in cell morphology and cell cycle.
Phenotypic and molecular features of Thai patients with primary carnitine deficiency
Authors: Liammongkolkul Somporn , Boonyawat Boonchai , Vijarnsorn Chodchanok , Tim‐Aroon Thipwimol , Wasant Pornswan , Vatanavicharn Nithiwat 
Published: November 2022

AbstractBackgroundPrimary carnitine deficiency (PCD) is screened by expanded newborn screening (NBS) using tandem mass spectrometry (MS/MS) that can detect both affected neonates and mothers. This study aimed to delineate the clinical, biochemical, and molecular findings of Thai PCD patients.MethodsExpanded NBS using MS/MS was implemented in Bangkok and 146,757 neonates were screened between 2014 and 2018. PCD was screened by low free carnitine (C0) levels in dried blood spots. Plasma C0 levels and C0 clearance values were measured in neonates and their mothers with positive screening results. Clinically diagnosed cases were described. The coding regions and intron‐exon boundaries of the SLC22A5 gene were sequenced in all cases with low plasma C0 levels.ResultsThere were 14 cases with confirmed PCD: two clinically diagnosed cases, and 12 cases identified through NBS including five newborns, six mothers, and one older sibling. Thus, the incidence of PCD in neonates was 1:29,351. All affected neonates and mothers were asymptomatic except one mother with dilated cardiomyopathy. SLC22A5 gene sequencing identified biallelic causative variants in all cases, comprising 10 different variants of which four were novel. c.51C > G (p.Phe17Leu) and c.760C > T (p.Arg254Ter) were the most prevalent variants in this study. Cases with significant clinical features tended to have higher C0 clearance values.ConclusionsPrimary carnitine deficiency is a common inherited metabolic disorder (IMD) in Thailand. Our findings broaden the spectrum of SLC22A5 variants. The future national NBS program will shed more light on PCD and other IMDs in Thailand.