bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromogenic Escherichia coli reporter strain for screening DNA damaging agents
Authors: Mora-Garduño Josué Daniel , Tamayo-Nuñez Jessica , Padilla-Vaca Felipe , Ramírez-Montiel Fátima Berenice , Rangel-Serrano Ángeles , Santos-Escobar Fernando , Gutiérrez-Corona Félix , Páramo-Pérez Itzel , Anaya-Velázquez Fernando , García-Contreras Rodolfo , Vargas-Maya Naurú Idalia , Franco Bernardo 
Published: January 2022

AbstractThe presence of pollutants in soil and water has given rise to diverse analytical and biological approaches to detect and measure contaminants in the environment. Using bacterial cells as reporter strains represents an advantage for detecting pollutants present in soil or water samples. Here, an Escherichia coli reporter strain expressing a chromoprotein capable of interacting with soil or water samples and responding to DNA damaging compounds is validated. The reporter strain generates a qualitative signal and is based on the expression of the coral chromoprotein AmilCP under the control of the recA promoter. This strain can be used simply by applying soil or water samples directly and rendering activation upon DNA damage. This reporter strain responds to agents that damage DNA (with an apparent detection limit of 1 µg of mitomycin C) without observable response to membrane integrity damage, protein folding or oxidative stress generating agents, in the latter case, DNA damage was observed. The developed reporter strain reported here is effective for the detection of DNA damaging agents present in soils samples. In a proof-of-concept analysis using soil containing chromium, showing activation at 15.56 mg/L of Cr(VI) present in soil and leached samples and is consistent with Cr(III) toxicity at high concentrations (130 µg). Our findings suggest that chromogenic reporter strains can be applied for simple screening, thus reducing the number of samples requiring analytical techniques.
Survival analysis of neonatal mortality in Ghana using three population-based surveys
Authors: Doku David Teye 
Published: January 2022

Abstract
Background
Neonatal mortality in many low-and middle-income countries (LMICs) remains high despite global efforts at addressing this challenge. Tackling neonatal death in LMICs is further complicated by lack of reliable data from individual countries in the region to inform effective context specific interventions. This study investigates the probability of neonatal survival and socio-demographic risk factors of neonatal mortality in Ghana.

Methods
Pooled data from three population-based surveys (N = 12,148) were analysed using multivariable Cox Proportional Hazards regression models.

Results
The risk of dying within the first 28 days of life was highest in the first week of life (early neonatal period), it then decreases sharply around the middle of the second week of life and remains low over the late neonatal period. Adjusted hazard ratios (HRs) showed that: rural residency (HR = 1.80, 95% CI: 1.15-2.75); birth order 2-3 (HR = 1.63, 95% CI: 1.10-2.42); birth order ≥7 (HR = 1.89, 95% CI: 1.07-3.33) increased the risk of neonatal death. Additionally, children born to women who were obese had higher risk of neonatal death (HR = 1.69, CI: 1.12-2.56) compared with those of women with optimal weight. Disparities in the risk of neonatal death by geographical regions were also found.

Conclusion
The risk of neonatal mortality is highest during the first week of life and it is socio-demographically patterned. The findings emphasise the need to tackle socio-demographic risk factors of neonatal mortality in order to achieve the Sustainable Development Goal 3, which is aimed at reducing neonatal mortality to 12 per 1000 live births by the year 2030.
Extensive outbreak of colistin resistant, carbapenemase (blaOXA-48, blaNDM) producing Klebsiella pneumoniae in a large tertiary care hospital, India
Authors: Sharma Swati , Banerjee Tuhina , Kumar Ashok , Yadav Ghanshyam , Basu Sriparna 
Published: January 2022

Abstract
Background
Extensive drug resistance in Klebsiella pneumoniae (K. pneumoniae) causing major outbreaks in large hospitals is an emerging challenge. We describe a near fatal outbreak of colistin resistant, carbapenem resistant K. pneumoniae (CRKp) producing metallo beta-lactamases (blaNDM) and blaOXA-48 in the neonatal intensive care unit (NICU) at the background of a larger outbreak involving multiple parts of the hospital and the challenges in its containment.

Methods
Following identification of an outbreak due to colistin resistant CRKp between April to June 2017 in the NICU, a thorough surveillance of similar cases and the hospital environment was performed to trace the source. All the isolated K. pneumoniae were tested for susceptibility to standard antibiotics by disc diffusion and microbroth dilution methods. Molecular detection of extended spectrum beta lactamases (ESBLs) and carbapenemases (classes A, B, D) genes was done. Enterobacterial repetitive intergenic consensus (ERIC) PCR and multi-locus sequence typing (MLST) was done to determine the genetic relatedness of the isolates. Characteristics of different sequence types were statistically compared (Student’s t-test).

Results
A total of 45 K. pneumoniae isolates were studied from NICU (14 cases of neonatal sepsis), ICU (18 cases), other wards (7 cases) along with 6 isolates from hospital environment and human colonizers. The primary case was identified in the ICU. All the K. pneumoniae from NICU and 94.4% from the ICU were colistin resistant CRKp. Majority (59.37% and 56.25%) harbored blaSHV/blaCTXM and blaOXA-48 genes, respectively. Two distinct sequence types ST5235 and ST5313 were noted with colistin resistance, distribution within the NICU and mortality as significant attributes of ST5235 (p < 0.05). The outbreak was contained with strengthening of the infection control practices and unintended short duration closure of the hospital.

Conclusion
Large hospital outbreaks with considerable mortality can be caused by non-dominant clones of colistin resistant CRKp harboring blaOXA-48 and blaNDM carbapenemases in endemic regions. The exact global impact of these sequence types should be further studied to prevent future fatal outbreaks.
Borderline personality disorder in young people: associations with support and negative interactions in relationships with mothers and a best friend
Authors: Hessels Christel J. , van den Berg Tessa , Lucassen Sofie A. , Laceulle Odilia M. , van Aken Marcel A. G. 
Published: January 2022

Abstract
Background
Impaired interpersonal functioning has been highlighted as a core feature of borderline personality disorder (BPD). Adolescence and young adulthood form important developmental stages within both the emergence of BPD and the development of interpersonal functioning, which takes place mostly in relationships with parents and friends. This study aimed to: (i) investigate relations between BPD symptoms and both supportive and negative interactions with mothers and best friends; (ii) investigate whether the relations were moderated by age; (iii) test the robustness of our findings by comparing the results based on self-reports with results from a subsample in which supportive and negative interactions with mothers were rated by the mother.

Methods
312 young people referred to mental healthcare completed self-report measures on BPD and supportive and negative interactions. Multiple regression analyses were conducted to examine the relations between BPD features and perceived supportive and negative interactions with mothers and a best friend, and to investigate whether these relations were moderated by age. Robustness of our findings was studied in a subsample (n = 104), by using a multi-informant design in maternal report on supportive and negative interactions with mothers.

Results
Multiple regression analyses demonstrated that negative interactions with mothers as well as with a best friend were related to more BPD symptoms in young people. Supportive interactions were not related to BPD symptoms. Both BPD and quality of relations were not related to age. In a subsample in which supportive and negative interactions with mothers were rated by the mother, the maternal report showed slightly different results. In this model, both supportive and negative interactions with a best friend were positively related, whereas interactions with mothers were not related to BPD symptoms in young people.

Conclusions
Results highlight the importance of relationships with mothers and a best friend during adolescence and young adulthood. Given that BPD often emerges during this developmental phase, future research is needed to clarify how quality of relationships could alter pathways toward BPD in young people.

Trial registration
Not applicable.
Birdshot retinochoroiditis in Brazil: a multicenter review of 40 patients
Authors: da Fonsêca Maria Luisa Gois , Vianna Raul N. G. , Rocha Anna C. H. , Casella Antonio M. B. , Cialdini Arnaldo , Muccioli Cristina , da Costa Daniela S. , Lucena Daniel R. , Vasconcelos-Santos Daniel V. , Morizot Eduardo , Castro Elaine , Rodriguez Ever E. C. , Diligenti Felipe T. , Porto Fernanda B. O. , Nascimento Heloisa , Yanamoto Joyce H. , Oréfice Juliana L. , Muralha Lilia R. P. , Carneiro Luciana B. , Finamor Luciana P. S. , Frazão Maria A. M. , Motta Mario , Nobrega Mario J. , Zajdenweber Moyses E. , Moraes Remo T. , Meirelles Rodrigo L. , Lemos Sidney R. , de Araújo Wilton Feitosa 
Published: January 2022

Abstract
Background
Birdshot retinochoroiditis (BRC) is a rare and chronic bilateral uveitis mostly found in Caucasians. As few data are available about the clinical course of BRC in Hispanic patients, we aimed to report the clinical findings and the evolution of BRC in Brazilian patients.

Methods
This retrospective cohort multicenter nationwide study was performed by analyzing the records of patients with BRC diagnoses from Brazilian ophthalmological centers from April 1995 to May 2020.

Results
Forty patients (80 eyes) with a diagnosis of BRC were evaluated. The mean age was 53 years, and there was no sex predominance. All tested patients (34/40) were positive for HLA-A29. The diagnosis of BRC was made following the Levinson et al. criteria, and all ancillary tests were performed to exclude differential diagnoses. Clinical signs and symptoms, such as complications and treatment, were described.

Conclusions
BRC evolution in Brazilian patients seems to have some peculiarities that diverge from the published literature available about Caucasians, as AS inflammation is higher in this population.
Online student support: a framework for embedding support interventions into the online learning cycle
Authors: Rotar O. 
Published: January 2022

AbstractSupport is one of the crucial elements of online students’ success. Although many support strategies have been documented in the past, less is known at what stages of the learning cycle suggested interventions can be embedded into the online learning curriculum. This paper offers a systematic review of the 28 empirical studies on effective support strategies and interventions that are indexed by the SCOPUS database between 2010 and 2020. Following an Inclusive Student Services Process Model framework, identified strategies are allocated across different phases of student learning to indicate where and when they can be delivered to online students. The analysis suggests that the effectiveness of the support provision depends on the time when support is offered. Furthermore, it was found that two areas support delivery, namely support at transitions and measurement of support interventions, remain under-researched. Finally, the analysis showed two emerging trends in online students support: an increasing role of technology and social network sites to design support interventions and a shift to a more personalised yet holistic approach to student support.
Herbal medicine used for the treatment of diarrhea and cough in Kampala city, Uganda
Authors: Walusansa Abdul , Asiimwe Savina , Ssenku Jamilu. E. , Anywar Godwin , Namara Milbert , Nakavuma Jesca L. , Kakudidi Esezah K. 
Published: January 2022

Abstract
Background
Globally, diarrheal and respiratory diseases are among the main causes of mortality and morbidity. In Uganda, cities are facing proliferation of trade in herbal medicines (HM), including those for diarrhea and/or cough. Information on the economic, and the ethnopharmacological aspects of these HM is scarce, deterring the sector from achieving optimal capacity to support national development. We profiled the anti-diarrhea and/or anti-cough HM, and the basic economic aspects of HM trade in Kampala city, to support ethnopharmacological knowledge conservation and strategic planning.

Methods
A cross-sectional survey was conducted on 65 herbalists using semi-structured questionnaires. This was supplemented by an observational survey using a high-resolution digital camera. Data were collected following the guidelines for research on HM, established by Uganda National Drug Authority, and World Health organization.

Results
Eighty-four plant species from 41 families were documented. Fabaceae and Myricaceae had the highest number of species (9, 10.7% each). Citrus limon (L.) Osbeck was the most commonly cited for cough, with a relative frequency of citation (RFC) of 1.00, and its relative medical importance was not significantly different from the other top 5 species except for Azadirachta indica A.Juss (RFC = 0.87). Entada abyssinica A. Rich (RFC = 0.97) was the most cited for diarrhea. Trees (34, 40.5%) were mostly used, and mainly harvested from wild habitats (55.2%) in 20 districts across Uganda. These HM were mainly sold as powders and concoctions, in markets, shops, pharmacies, and roadside or mobile stalls. The highest prices were Uganda Shillings (UGX) 48,000 ($13.15)/kg for Allium sativum L, and UGX 16,000 ($4.38)/kg for C. limon. All participants used HM trade as a sole source of basic needs; majority (60.0%) earned net monthly profit of UGX. 730,000 ($200) ≤ 1,460,000 ($400). The main hindrances to HM trade were the; disruptions caused by the COVID-19 pandemic (n = 65, 100%), and the scarcity of medicinal plants (58, 89.2%).

Conclusion
There is a rich diversity of medicinal plant species traded in Kampala to treat diarrhea and cough. The HM trade significantly contributes to the livelihoods of the traders in Kampala, as well as the different actors along the HM value chain throughout the country.
A review of stabilization methods for DCMG with CPL, the role of bandwidth limits and droop control
Authors: Ansari Sarah , Zhang Jing , Singh Rajat Emanuel 
Published: January 2022

AbstractDC microgrids (DCMGs) integrate and coordinate various DC distribution generation units including various renewable energy sources and battery storage systems, and have been used in satellites, the International Space Station, telecom power stations, computer power supplies, electric aircraft, and electric ships. However, the presence of constant power loads (CPLs) can cause instability in DCMGs. Thus, this paper reviews the stabilization techniques that can resolve instability caused by CPLs, as well as various parameters of CPLs, such as bandwidth, and the frequency of the CPLs that can stabilize the DCMGs. It also discusses recent trends and future work in finding stability limits using the parameters of CPLs. It should be useful for directing research towards appropriate mathematical and experimental approaches for the stability of DCMGs with CPLs.
Effect of endophytic Bacillus and arbuscular mycorrhiza fungi (AMF) against Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici
Authors: Devi Nongthombam Olivia , Tombisana Devi R. K. , Debbarma Manashi , Hajong Monika , Thokchom Sushanti 
Published: January 2022

AbstractBackgroundFusariumwilt of tomato caused byFusarium oxysporumf. sp.lycopersici(FOL) is a serious disease that causes significant economic losses in tomato production. Seventeen endophyticBacillusisolates from tomato roots of Meghalaya were tested for antagonistic and plant growth promotion activities. Dominating arbuscular mycorrhiza fungi (AMF) spores were isolated from the rhizosphere soils of tomato grown in Meghalaya. The effect of different combinations of AMF and endophyticBacillusonFusariumwilt severity and growth of tomato plant under pot and field conditions was studied.ResultsThe endophyticBacillusisolates ERBS51 and ERBS10 showed a maximum inhibition against FOL, with 58.43 and 55.68%, respectively, in a dual culture experiment. ERBS51 and ERBS10 were identified asBacillus velezensisandBacillussp., respectively, based on 16s rRNA sequencing. Both isolates were found positive for iturin A, surfactin, bacillomycin D, protease, cellulase, pectinase, alpha-amylase, siderophore, ammonia production and ZnCO3solubilization.Funneliformis mosseaeandGlomus fasciculatumwere the dominating AMF species in tomato rhizosphere of Meghalaya. The result of pot and field experiments revealed that out of all the treatments, combination ofFunneliformis mosseae + Glomus fasciculatum + Bacillus velezensis + Bacillussp. was shown to be the best in reducing the severity ofFusariumwilt to 77.44 and 66.74%, respectively.F. mosseae + G. fasciculatum + B. velezensis + Bacillussp. also recorded the highest in most growth attributes and yield.ConclusionsEndophyticBacillus(B. velezensisandBacillussp.) and AMF (F. mosseaeandG. fasciculatum) were safe and effective biocontrol agents againstFusariumwilt of tomato.
Kruppel-like factor 5 (Klf5) in fetal-maternal tissue during periimplantation and effects of ovarian steroid hormone antagonist on its expression during uterine receptivity of albino mice
Authors: Talukdar Kanmuna Ray , Saikia Purbajyoti , Sarma Hirendra N. 
Published: January 2022

Abstract
Background
Embryo implantation is a tightly regulated sequence of events regulated by ovarian steroids, estrogen and progesterone, and their downstream targets. Ovarian steroids regulate most of the genes involved in embryo implantation and pregnancy. However, some factors are not regulated by ovarian steroids, estrogen, progesterone, or both. Kruppel-like factor 5 (Klf5) is an example of an ovarian steroid–independent factor having a role in cellular proliferation, differentiation. The detailed expression profile of Klf5 during uterine receptivity and periimplantation has not been studied till now. In the present research work, an attempt was made to investigate the expression pattern of Klf5 in mice fetal-maternal tissue during periimplantation (day 4–day 8). The expressional and functional independence of Klf5 on the ovarian steroids was studied using estrogen and progesterone antagonist. The study was carried out in female Swiss albino mice of LACA strain during the periimplantation period. KLF5 was localized in the fetal-maternal tissues using the immunofluorescence technique in paraffin-embedded tissues. Ovarian steroid antagonists were administered subcutaneously from day 1 to day 3 of gestation, and the uterus was collected on the morning of day 4. Klf5 protein and mRNA levels were studied by western blot and quantitative real-time PCR (qPCR), respectively.

Results
KLF5 was localized in the embryo, uterine luminal epithelium, glandular epithelium, and proliferating stromal cells during periimplantation. In ovarian steroid antagonist–treated groups, KLF5 was localized in the luminal and glandular epithelium and stroma. Western blot and qPCR confirmed translation and transcription of KLF5 during the experimental period. The KLF5 protein level significantly increased on day 6, day 7, and day 8 when compared with day 4 (P < 0.05). The mRNA level of Klf5 increased significantly on day 7 and day 8 when compared with day 4 (P < 0.05). In ovarian steroid antagonist–treated groups, protein and mRNA corresponding to Klf5 were observed. From this finding, it can be assumed that Klf5 may be a steroid-independent factor expressed during uterine receptivity.

Conclusion
Spatiotemporal KLF5 expression in fetal-maternal tissue was observed during the experimental period. The results suggest that Klf5 is an ovarian steroid–independent factor that may play a pivotal role in implantation, decidualization, and embryogenesis.
Investigation of the correlation between cardiac parameters and aortic diameter in patients with ascending aortic aneurysm
Authors: Etli Mustafa , Avnioglu Seda , Yilmaz Halil , Karahan Oguz 
Published: January 2022

Abstract
Background
Aortic aneurysms (AA) are enlargement of the aorta silently until diagnosing, not detectable on physical examination, and usually incidentally discovered during radiologic scanning for other reasons. It can get bigger sizes and can result in life-threatening outcomes if not detected early on. In this study, we aimed to determine the relationship between ascending aortic diameter and cardiac parameters that can be detected with tomography or/and echocardiography. Newly diagnosed (n: 85) ascending AA patients and healthy individuals (n: 86) who have not any thoracic pathology in computed tomography (CT) scans included to the study. Echocardiographically determined left atrial dimension (LAD), left ventricular end-diastolic diameter (LVDd), left ventricular end-systolic diameter (LVDs), left ventricular ejection fraction (LVEF) and the left ventricular posterior wall thickness (LVPWd) values of each patient were recorded. The thorax diameters, ascending aorta diameters and cardiac volume values recorded from CT scans. The obtained findings were statistically compared.

Results
Positive correlation was found between aortic diameter and aging (p: 0.000). Increased thorax diameter and cardiac volume values were detected in ascending AA cases (p < 0.05). It was found to be ascending aortic diameter was positively correlated with thorax diameter and cardiac volume (0.50 < r ≤ 0.70) values and higher aortic diameter, cardiac volume, thorax diameter values were detected in male individuals when compared with the female gender. There was no significant correlation between LVEF, LVDd, and LVDs values and aortic diameter.

Conclusions
Cardiac volume and thorax diameter were found as strongly correlated with the diameter of the ascending aorta. The clarifying of these parameters with larger cohorts might be beneficial for the estimation of the progression of ascending AA.