bluuebunny/crossref_metadata_2025_split huggingface dataset:
FOXD3 and GAB2 as a pair of rivals antagonistically control hepatocellular carcinogenesis
Authors: Liu Ruimin , Sun Yan , Chen Shuai , Hong Yun , Lu Zhongxian 
Published: February 2022

Our previous study demonstrated that GAB2 promoted tumorigenesis in liver tissue and was a potential target for the treatment of hepatocellular carcinoma (HCC). Here, we identified that the tumour suppressor protein Forkhead box D3 (Foxd3) is a transcriptional repressor of the Gab2 gene. In human HCC cells, FOXD3 expression is low, but GAB2 expression is abundant. Increased Foxd3 expression inhibited the expression of Gab2 in a dose‐dependent manner. Ectopic expression of Foxd3 in HCC cells reduced Gab2‐mediated promotion of cell proliferation and migration in vitro. Foxd3 also inhibited Gab2‐stimulated phosphorylation of Jak2 and Stat3. Furthermore, the protein levels of Foxd3 and Gab2 had a clear negative correlation: Gab2 expression was induced, whereas Foxd3 expression was suppressed in most tumour tissues in mice with diethylnitrosamine (DEN)‐induced hepatocellular carcinoma. These results suggest that the tumour suppressor Foxd3 and tumour enhancer Gab2 mutually inhibit each other to synergistically control the occurrence of HCC, providing a novel mechanism for treating this disease.
A Corning perspective on the future of technical glass in our evolving world
Authors: Kohli Jeffrey T. , Hubert Mathieu , Youngman Randall E. , Morse David L. 
Published: February 2022

AbstractGlass is a material with a rich history and a bright future. Due to its outstanding properties and its versatility, it is a key material in many applications. In this paper, we present a variety of these applications in which glass is playing, and will continue to play, a significant role, including communication technologies, the semiconductor industry, advanced displays, mobile consumer electronics, healthcare, and automotive. Other critical considerations enabling continuous progress in glass science and engineering, such as improved modeling tools and characterization techniques, increased sustainability of the glass‐making process, and talent development, are also discussed.
Prediction of the rheological properties of wheat dough by starch‐gluten model dough systems: effect of gluten fraction and starch variety
Authors: Xu Fen , Liu Wei , Zhang Liang , Liu Qiannan , Hu Xiaojia , Wang Feng , Zhang Hong , Hu Honghai , Blecker Christophe 
Published: February 2022

SummaryThe present study sought to investigate the rheological properties of wheat starch‐gluten (WS‐G) and potato starch‐gluten (PS‐G) model doughs with different gluten fractions to elucidate the effectiveness of using model dough to predict wheat dough properties. The highest linear viscoelastic region, frequency dependence, maximum creep compliance and the lowest viscoelastic modulus and zero shear viscosity were observed in the wheat dough, followed by WS‐G and PS‐G model doughs. PS exerted a more significant damage effect on the gluten network while WS shared a tight integration with gluten protein, forming a more stable dough structure. The viscoelasticity of the model doughs shared a close association with the wheat dough under increased gluten fraction, while the frequency dependence of the model doughs showed no trend towards wheat dough. Therefore, starch‐gluten model dough could not fully stimulate the functionality of wheat dough irrespective of its gluten fraction.
Drosophila suzukii population dynamics and control efficiency of mineral dusts with a focus on grape protection
Authors: Dam Doriane , Molitor Daniel , Schultz Mareike , Beyer Marco 
Published: February 2022

AbstractThe invasive pest Drosophila suzukii is threatening berry production. It is mainly managed via chemical control, which is associated with consumer and environmental concerns. Here, we tested the efficacy of mineral dusts under field and laboratory conditions in 2019 and 2020. Furthermore, population dynamics were studied in a vineyard and its surroundings. The kaolin products Cutisan and Surround®, as well as the CaCO3 product Carboliq, had neither insecticidal nor repellent effects on Drosophila suzukii adults in laboratory choice tests with grapes at concentrations of up to 2% (w/v). Cutisan and Surround® significantly reduced the number of deposited eggs (−41.9% and −49.3% respectively) while Carboliq had no effect on the oviposition under laboratory conditions. The Surround® treatment significantly reduced the number of flies trapped on 09 September 2020 at a test vineyard. Depending on the assessment date and treatment, between 59% and 84% of the flies in the bait traps were females. The number of eggs found in fruit treated with Carboliq in the field was higher at each assessment date than in the control but this difference was not statistically significant. Fruit treated with Cutisan or Surround® in the field showed an equivalent or lower average number of eggs compared with the control, but this difference was only significant on 24 September 2020. Between May 2015 and October 2020, the highest number of D. suzukii adults was observed around September in the field and a decline of the population occurred in the winter months until July. In epidemic years, temperature – humidity – combinations prior to population peaks were quite stable with low humidity being associated with a high temperature and vice versa. In non‐epidemic years, humidity fluctuated more than in epidemic years and temperatures were lower before population peaks. The effect of global radiation on population maxima seemed to be minor.
Reducing potential errors associated with insulin administration: An integrative review
Authors: Alqahtani Naji 
Published: February 2022

AbstractRationale, Aims, ObjectivesAround one‐third of medication errors resulting in death within 48 h involve insulin therapy. Despite a growing number of interventional strategies that have been published over the past decade, it remains unclear which of these interventions is effective in reducing insulin errors. Therefore, the study aimed to synthesize interventions to reduce the frequency of insulin errors in either home or health care settings.MethodsThis integrative review was conducted based on Whittemore and Knafl's four steps, which includes problem identification, literature search, data analysis and presentation. Six databases including Cumulative Index of Nursing and Allied Health Literature (CINAHL), Medline, PubMed, Scopus, PsychInfo and Cochrane were searched from January 2010 through July 2021. The level of evidence quality was assessed according to the Johns Hopkins Nursing Evidence‐Based Practice grading scale.ResultsSixteen studies meeting inclusion criteria were reviewed. The results provide strong support for teaching patients how to use automated bolus calculators and educating patients to self‐administer insulin to prevent insulin errors in the home setting. Computerized protocols, education and double‐checking procedures were also found to be effective strategies for minimizing insulin errors in healthcare settings.ConclusionWhile the strategies might be effective in reducing insulin administration errors in the home settings, computerized protocols, continuing education and the manual validation of insulin products appear to be the most effective strategies for reducing such insulin errors in healthcare settings. Understanding these findings may help clinicians and patients to decrease the number of insulin errors administration.
Macronuclear development in ciliates, with a focus on nuclear architecture
Authors: Ahsan Ragib , Blanche Wumei , Katz Laura A. 
Published: February 2022

AbstractCiliates are defined by the presence of dimorphic nuclei as they have both a somatic macronucleus and germline micronucleus within each individual cell. The size and structure of both germline micronuclei and somatic macronuclei vary tremendously among ciliates. Except just after conjugation (i.e. the nuclear exchange in their life cycle), the germline micronucleus is transcriptionally inactive and contains canonical chromosomes that will be inherited between generations. In contrast, the transcriptionally active macronucleus contains chromosomes that vary in size in different classes of ciliates, with some lineages having extensively fragmented gene‐sized somatic chromosomes while others contain longer multigene chromosomes. Here, we describe the variation in somatic macronuclear architecture in lineages sampled across the ciliate tree of life, specifically focusing on lineages with extensively fragmented chromosomes (e.g. the classes Phyllopharyngea and Spirotrichea). Further, we synthesize information from the literature on the development of ciliate macronuclei, focusing on changes in nuclear architecture throughout life cycles. These data highlight the tremendous diversity among ciliate nuclear cycles, extend our understanding of patterns of genome evolution, and provide insight into different germline and somatic nuclear features (e.g. nuclear structure and development) among eukaryotes.
Paragangliomas of the head and neck
Authors: Valero Cristina , Ganly Ian 
Published: February 2022

AbstractParagangliomas are rare neuroendocrine tumors that can be found from the skull base to the pelvis. Head and neck paragangliomas have been historically treated with surgery. However, surgical resection adds risk of injury to vascular structures and cranial nerves that can lead to morbidity such as hoarseness, dysarthria, dysphagia, or aspiration. Recently, improved understanding of the behavior of these tumors and increasing experience in non‐surgical treatments, such as observation and radiation therapy, have changed the paradigms of management of this entity. Multiple series now show a trend toward a more conservative management, with a higher percentage of patients being observed or treated with radiotherapy. Several factors should be taken into consideration when deciding the most appropriate treatment for head and neck paragangliomas, starting by differentiating carotid body tumors from non‐carotid body tumors. In general, surgical resection is normally recommended for carotid body tumors as the complications from treatment are usually minimal. In contrast, for non‐carotid body tumors, surgery is often associated with significant functional impairment due to cranial nerve paralysis. As such, non‐surgical treatment is now usually recommended for this subset of head and neck paragangliomas. In young patients with no comorbidities and a small to medium carotid body tumors, surgery should be considered. Moreover, surgery should be offered for secreting tumors, malignant tumors, tumors with rapid growth or increase in symptomatology, and when radiotherapy cannot be performed. Conversely, conservative management with active surveillance or radiotherapy can be offered in the remaining cases in order to avoid unnecessary morbidity while still providing acceptable tumor control.
Process and Product in ISLA Research: Courage, Commitment, and Tolerance for Ambiguity
Authors: Ranta Leila 
Published: February 2022

AbstractStern (1983) reminds us of the ethical reasons for doing second language (L2) research. That is, given the considerable human and financial investments that go into language education, the practical activities of teaching “should not exclusively rely on tradition, opinion, or trial‐and‐error but should be able to draw on rational enquiry, systematic investigation, and, if possible, controlled experiment” (p. 57). Elsewhere Stern argues for the use of interdisciplinary teams to carry out such research. The studies in this special issue illustrate the aptness of Stern's advice. These articles present findings from a large‐scale classroom research project that compared a deductive approach to teaching Spanish grammar to guided induction using the PACE model. The multidisciplinary team made use of different types of data, which were examined through different theoretical lenses. This discussion article considers the implications of these studies for L2 research, educational practice, teacher education, and the relationship between theory and practice.
Sperm competition intensity affects sperm precedence patterns in a polyandrous gift‐giving spider
Authors: Matzke Magdalena , Toft Søren , Bechsgaard Jesper , Pold Vilstrup Astrid , Uhl Gabriele , Künzel Sven , Tuni Cristina , Bilde Trine 
Published: February 2022

AbstractSperm competition drives traits that enhance fertilization success. The amount of sperm transferred relative to competitors is key for attaining paternity. Female reproductive morphology and male mating order may also influence fertilization, however the outcome for sperm precedence under intense sperm competition remains poorly understood. In the polyandrous spider Pisaura mirabilis, males offer nuptial gifts which prolong copulation and increase sperm transfer, factors proposed to alter sperm precedence patterns under strong sperm competition. First, we assessed the degree of female polyandry by genotyping wild broods. A conservative analysis identified up to four sires, with a mean of two sires per brood, consistent with an optimal mating female rate. Then we asked whether intense sperm competition shifts sperm precedence patterns from first male priority, as expected from female morphology, to last male advantage. We varied sexual selection intensity experimentally and determined competitive fertilization outcome by genotyping broods. In double matings, one male monopolised paternity regardless of mating order. A mating order effect with first male priority was revealed when females were mated to four males, however this effect disappeared when females were mated to six males, probably due to increased sperm mixing. The proportion of males that successfully sired offspring drastically decreased with the number of competitors. Longer copulations translated into higher paternity shares independently of mating order, reinforcing the advantage of traits that prolong copulation duration under intense competition, such as the nuptial gift. Sperm competition intensity enhances the impact of competitive sexual traits and imposes multiple effects on paternity.
Human milk oligosaccharide profiles and child atopic dermatitis up to 2 years of age: The Ulm SPATZ Health Study
Authors: Siziba Linda P. , Mank Marko , Stahl Bernd , Kurz Deborah , Gonsalves John , Blijenberg Bernadet , Rothenbacher Dietrich , Genuneit Jon 
Published: February 2022

AbstractBackgroundHuman milk oligosaccharides (HMOs) have several biological functions. Yet, very few studies have investigated the effect of HMOs on the development of allergies and even fewer on their specific associations with atopic dermatitis (AD) during early childhood.ObjectiveThis study investigated whether individual HMO concentrations, measured at two time points of lactation, were associated with reported diagnosis of AD in children up to two years of age.MethodOutcome data were available for HMOs measured in human milk samples collected at 6 weeks (n = 534) and 6 months (n = 356) of lactation. Associations of HMOs with AD, ascertained from parents and pediatricians at ages one and two years, were assessed in crude and adjusted logistic regression models.ResultsFew associations were statistically significant at the conventional level (p < .05), for example, 6‐week Lacto‐N‐neotetraose with 2‐year AD [OR 95%CI: 0.82 (0.66, 1.00)] and 6‐month 3'‐sialyllactose among non‐secretor mothers with 1‐year AD [2.59 (1.53, 6.81)]. Importantly, accounting for multiple testing, these and all further associations were not statistically significant (all p > .0031, which is the threshold for statistical significance after correction for multiple testing).ConclusionOur findings suggest that the intake of different levels (or even absence) of the individual HMOs measured at 6 weeks and 6 months of lactation, in the current study, is not significantly associated with the development of AD in early childhood. Given the exploratory nature of our study and the limited sample size, these results should be interpreted with caution. The specific HMOs for which we show plausible associations at conventional level may warrant further research and investigation.
A novel pathogenic RHOA variant in a patient with patterned cutaneous hypopigmentation associated with extracutaneous findings
Authors: Cai Zhuo Ran , McCuaig Catherine , Hatami Afshin , Rivière Jean‐Baptiste , Marcoux Danielle 
Published: February 2022

AbstractRHOA‐related neuroectodermal syndrome is characterised by linear skin hypopigmentation along Blaschko's lines associated with alopecia, leukoencephalopathy, facial and limb hypoplasia, and ocular, dental, and acral anomalies. Herein, we report a patient with patterned cutaneous hypopigmentation with a similar phenotype due to a novel postzygotic RHOA variant (c.210G>T; p.Arg70Ser). This illustrates that the complexity of the orchestration of morphogenesis and organogenesis can be affected by different variants in the same gene.
Treatment of scarring alopecia in children using follicular unit hair transplantation
Authors: Jin Fei , Wang Yanni , Wang Yining , Qi Hongyan , Wang Jiping 
Published: February 2022

AbstractBackgroundScarring alopecia can significantly affect children emotionally. Follicular unit excision (FUE) and follicular unit transplantation (FUT) have been applied for scar treatment.ObjectiveThis study aimed to evaluate the safety and feasibility of follicular unit hair transplantation in treating scarring alopecia in children.Patients and MethodsA total of nine children (seven males and two females) with cicatricial alopecia, ranging in age from 5 years, 2 months to 12 years, 10 months were included in this study. Scar formation time ranged from 7 months to 5 years. Sites were vertex (2), eyebrow (3), frontal hairline (3), and temporal regions (2).ResultsNine children in this group were followed up for 6–34 months with the following treatment options: FUE (5 cases), FUT (3 cases), and FUT combined with FUE (1 case). No significant complications were observed during the treatment. The transplanted hair grew well, the direction and shape were satisfactory, and the survival rate was >90%.ConclusionFor children with burn trauma and cicatricial alopecia after surgery, hair transplantation can significantly improve their appearance with low surgical risk and high patient satisfaction rate.
Early‐onset juvenile dermatomyositis: A report of two cases and review of the literature
Authors: Barrón‐Calvillo Elsa Eduwiges , García‐Romero María Teresa 
Published: February 2022

AbstractJuvenile dermatomyositis (JDM) is an uncommon disease in children younger than 3 years of age. The clinical manifestations may be different than in older children, often delaying the diagnosis. We present two patients with early‐onset JDM and review the literature describing the unique clinical characteristics in this age group.
Management of pericatheter dialysate leaks using hemostatic agents
Authors: Eray İsmail Cem , Topal Ugur , Balal Mustafa , Paydas Saime 
Published: February 2022

AbstractContinuous ambulatory peritoneal dialysis (CAPD) is one of the treatment modalities used in end‐stage renal disease. The most common cause of failure is catheter‐related complications. Dialysate leak represents a major noninfectious complication of PD. Here, we aimed to present the results of patients who had pericatheter dialysate leak following PD catheterization and who were administered a hemostatic agent (HaemoCer Plus, BioCer, Germany) around the catheter and the tunneled segment under the subcutaneous tissue under local anesthesia. We performed a local procedure on six patients in total. No major complications developed in any of the patients at postoperative follow‐up. Five patients started to receive PD uneventfully within postoperative 3 days with no dialysate leak. We believe that this practice is effective in the management of peritoneal dialysate leak. The hemostatic agents administered in the present study can manage dialysate leak and ensure safe use of the catheter.
Effect of childhood developmental coordination disorder on adulthood physical activity; Arvo Ylppö longitudinal study
Authors: Tan Jocelyn L. K. , Ylä‐Kojola Anna‐Mari , Eriksson Johan G. , Salonen Minna K. , Wasenius Niko , Hart Nicolas H. , Chivers Paola , Rantalainen Timo , Lano Aulikki , Piitulainen Harri 
Published: February 2022

Individuals at risk of Developmental Coordination Disorder (DCD) have low levels of physical activity in childhood due to impaired motor competence; however, physical activity levels in adulthood have not been established. This study sought to determine the impact of DCD risk on physical activity levels in adults using accelerometry measurement. Participants (n = 656) from the Arvo Ylppö Longitudinal Study cohort had their motor competence assessed at the age of five years, and their physical activity quantified via device assessment at the age of 25 years. Between group differences were assessed to differentiate physical activity measures for individuals based on DCD risk status, with general linear modeling performed to control for the effects of sex, body mass index (BMI), and maternal education. Participants at risk of DCD were found to have a lower total number of steps (d = 0.3, p = 0.022) than those not at risk. Statistical modeling indicated that DCD risk status increased time spent in sedentary light activity (β = 0.1, 95% CI 0.02 to 0.3, p = 0.026) and decreased time spent in vigorous physical activity via interaction with BMI (β = 0.04, 95% CI 0.001 to 0.1, p = 0.025). Sensitivity analysis found that visuomotor impairment did not significantly impact physical activity but did increase the role of DCD risk status in some models. This 20‐year‐longitudinal study indicated that DCD risk status continues to negatively impact on levels of physical activity into early adulthood.