bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neutrophil extracellular traps facilitate cancer metastasis: cellular mechanisms and therapeutic strategies
Authors: Hu Wenxing , Lee Serene M. L. , Bazhin Alexandr V. , Guba Markus , Werner Jens , Nieß Hanno 
Published: September 2022

Abstract
Background
The formation of neutrophil extracellular traps (NETs) was initially discovered as a novel immune response against pathogens. Recent studies have also suggested that NETs play an important role in tumor progression. This review summarizes the cellular mechanisms by which NETs promote distant metastasis and discusses the possible clinical applications targeting NETs.

Method
The relevant literature from PubMed and Google Scholar (2001–2021) have been reviewed for this article.

Results
The presence of NETs has been detected in various primary tumors and metastatic sites. NET-associated interactions have been observed throughout the different stages of metastasis, including initial tumor cell detachment, intravasation and extravasation, the survival of circulating tumor cells, the settlement and the growth of metastatic tumor cells. Several in vitro and in vivo studies proved that inhibiting NET formation resulted in anti-cancer effects. The biosafety and efficacy of some NET inhibitors have also been demonstrated in early phase clinical trials.

Conclusions
Considering the role of NETs in tumor progression, NETs could be a promising diagnostic and therapeutic target for cancer management. However, current evidence is mostly derived from experimental models and as such more clinical studies are still needed to verify the clinical significance of NETs in oncological settings.
Pediatric endoscopic subcutaneous mastectomy (pesma) with liposuction in adolescents with gynecomastia
Authors: Varlet François , Esposito Ciro , Scalabre Aurelien , Lepore Benedetta , Vermersch Sophie , Escolino Maria 
Published: September 2022

Abstract
Background
Surgical techniques for treatment of gynecomastia are increasingly less invasive. We described technical standardization of pediatric endoscopic subcutaneous mastectomy (PESMA) with liposuction.

Methods
All adolescents with primary gynecomastia, operated using PESMA with liposuction over the period June 2014–July 2021, were included. The video recording of procedures was analyzed to standardize the operative technique. After patient installation, 3 trocars were placed on the mid-axillary line. The technique included 5 steps: (1) subcutaneous injection of lipolysis solution and liposuction; (2) creation of working space using an inflated balloon; (3) gland dissection using 5-mm sealing device; (4) specimen extraction through the largest trocar orifice; and (5) placement of suction drainage tube.

Results
Twenty-four male adolescents, operated for Simon’s grade 2B and 3 gynecomastia using PESMA with liposuction over the study period, were included. Mean patient age was 16 years (range 15–18). Gynecomastia was bilateral in 19/24 (79.2%) and unilateral in 5/24 (20.8%). One (4.1%) conversion to open was reported. The mean operative time was 87 min (range 98–160) for unilateral and 160 min (range 140–250) for bilateral procedure. The mean length of stay was 2.2 days (range 1–4). Patients wore a thoracic belt for 15 up to 30 days postoperatively. Post-operative complications occurred in 5/24 (20.8%): 2- or 3 mm second-degree burns in 4 (16.7%) and subcutaneous seroma in 1 (4.1%). All complications were Clavien 2 grade and did not require further treatment. Aesthetic outcomes were very good in 21/24 (87.5%). Three (12.5%) boys had persistent minimal breast asymmetry but did never perceive it negatively.

Conclusion
PESMA combined with liposuction was feasible and safe for surgical treatment of gynecomastia in this selected cohort of patients. Although challenging, this procedure provided good aesthetic results, with no scars on the anterior thoracic wall. Standardization of the operative technique was a key point for successful outcome.
Involvement of adult children in treatment decision-making for older patients with cancer — a qualitative study of perceptions and experiences of oncology surgeons and nurses
Authors: Dijkman Bea L. , Paans Wolter , Van der Wal-Huisman Hanneke , van Leeuwen Barbara L. , Luttik Marie Louise 
Published: September 2022

Abstract
Background
Many older patients with cancer have their family members, often their adult children, involved in a process of treatment decision-making. Despite the growing awareness that family members can facilitate a process of shared decision-making, strategies for involving family members are scarce. Furthermore, literature about shared decision-making pays little attention to family involvement or to the impact that family relations have on the decision process. The purpose of this study was to explore how surgeons and nurses perceive the involvement of adult children of older patients with cancer in treatment decision-making. Subsequently, it identified strategies to ensure family involvement in the decision-making process, used in clinical practice.

Methods
Qualitative open in-depth interviews were conducted with 13 surgeons and 13 nurses working in a university or general hospital. Qualitative content analysis was conducted according to the steps of thematic analysis.

Results
Both nurses and surgeons indicated that adult children’s involvement in decision-making about treatment increases when patients become frail. They mentioned several characteristics of adult children’s behaviour during the decision-making process. Most of these characteristics are beneficial, but they also can be challenging. The distinct nature of adult children’s involvement can help older patients with cancer reach better-informed treatment decisions. Health professionals reported six strategies to support positive family involvement in decision-making about treatment.

Conclusion
Adult children may facilitate a process of shared decision-making and help patients reach well-informed treatment decisions. Health professionals’ strategies deliberately support positive family involvement.
Similar collagen distribution in full-thickness skin grafts in intraperitoneal and onlay positions, an experimental mice-study
Authors: Winsnes A. , Ivarsson M.-L. , Falk P. , Gunnarsson U. , Strigård K. 
Published: September 2022

Abstract
Purpose
Autologous full-thickness skin grafting (FTSG) has the potential to become an option in abdominal wall repair. An understanding of tissue remodelling in the extracellular matrix (ECM) is crucial as this interplay determines such parameters as tissue strength and flexibility. This cross-sectional preclinical laboratory study in mice provides information on the distribution of collagen types and matrix metalloproteinases (MMPs) in the ECM of FTSGs in the intraperitoneal and onlay positions compared with internal controls. The aim was to evaluate morphologic changes after tissue remodelling and repair in FTSGs applied in the two positions and to detect any adverse host response.

Methods
ECM components were evaluated as follows: qualitative examination of collagen bundle thickness using Picrosirius Red staining (collagen types I, III and IV); and evaluation of collagen types IV and V, as well as MMPs 1, 8 and 9 using immunohistochemical staining. Full-thickness grafts transplanted between female twin mice were examined as this best mimics autologous transplantation.

Results
At 8 weeks, FTSGs in the intraperitoneal position did not show any noticeable differences in morphologic appearance to those in the onlay position. Both intraperitoneal and onlay FTSGs showed increases in the amount of thick collagen bundles compared to internal controls. No correlation was seen between distribution of MMPs 1, 8 or 9 and distribution of collagen types I, III, IV or V.

Conclusion
This preclinical study shows that FTSGs in both intraperitoneal and onlay positions are possible application site options and, by extension, promising application site options for abdominal wall reinforcement in hernia surgery. Clinical studies in humans are required to confirm these findings.
Should they stay or should they go? Negative interest rate policies under review
Authors: Beckmann Joscha , Gern Klaus-Jürgen , Jannsen Nils 
Published: September 2022

AbstractNegative interest rate policies (NIRP) have become an established monetary policy instrument in the toolkit of the ECB. We discuss NIRP in the euro area based on theoretical considerations and available empirical evidence. We find that NIRP had some positive impact on loan growth and investment in the euro area, but that the room to further loosen monetary policy via NIRP may be small. NIRP is discussed also in the context of the general monetary policy environment.
Loss of support assessment for precast portal frames with friction connections and masonry infills
Authors: Labò Simone , Eteme Minkada Marius , Marini Alessandra , Belleri Andrea 
Published: September 2022

AbstractThe loss of support of beam elements in Italian precast buildings is one of the main vulnerabilities recorded in past earthquakes. The reason for beam falling is due to the absence of adequate structural details in the beam-column joints, which were typically relying on friction for buildings located in regions previously classified as non-seismic prone. To guarantee the structural safety of the whole existing building stock, this collapse mechanism must be further investigated; in this regard, it could be interesting to evaluate the level of safety achieved by simple beam-column friction connections to allow, for instance, the prioritization of the retrofit interventions to guarantee a minimum level of safety in a wide range of structures. The paper investigates the influence of the parameters governing the beam-column relative displacements in the case of friction connections to establish criteria for evaluating the seismic loss of support probability in existing precast buildings. A simplified analytical model is introduced to describe single and double portal frames with and without the presence of stiff masonry infills. The influence of the epicentral distance on the results obtained is also preliminary addressed. Nonlinear time history analyses are carried out on a 2D finite element model to validate the effectiveness of the simplified model taking as reference a building that experienced the loss of support of a main beam during a past earthquake.
Numerical and analytical methods for the design of beam-column sub-assemblies with composite deck slab
Authors: Chaudhari Tushar , MacRae Gregory , Bull Des , Clifton G. Charles , Hicks Stephen J. 
Published: September 2022

AbstractA finite element numerical model is used to estimate the force displacement backbone curve of steel moment frame beam-column-joint subassemblies tested at large scale under repeated cyclic loading with different composite deck slab configurations. The slab configurations included: no-slab; fully isolated slab; two configurations for detailing the slab near the region between the column flanges; and a full depth reinforced slab. Then, a simple analytical model was developed so that designers can estimate the likely peak strength due to slab effect. This analytical model considers the common deformation modes and the strength hierarchy. It was found that the finite element numerical model captured the backbone envelope of experimental tests done on different slab configurations for the bare frame, isolated slab and full depth slab configurations, but overestimated the strength at larger displacements for the other configurations due to difficulty in considering the slab modes of failure. The simple analytical model considered the nonlinear deformation modes of steel beam plastic hinging, concrete crushing both outside the column flange and within the beam flanges, slab shear fracture between the column flange tips, slab longitudinal and lateral reinforcement yielding as it carried the subassembly moments and transferred forces between the steel frame and the slab, and shear stud deformation. The proposed analytical model matched the experimental strengths and failure modes. The proposed finite element model is suitable for research, and the analytical model matched the experimental results, and is suitable for consideration in design.
Reproductive behaviors promote ecological and phenotypic sexual differentiation in the critically endangered Lehmann’s poison frog
Authors: Betancourth-Cundar Mileidy , Palacios-Rodriguez Pablo 
Published: September 2022

AbstractTerritoriality and parental care are complex reproductive behaviors found in many taxa from insects to mammals. Parental care can be carried out by the female, the male, or both, depending on the species. Territoriality, in contrast, is predominantly displayed by males. Different selective pressures imposed on individuals from the sex performing territorial or parental care behaviors may also lead to sexual differentiation in other life-history traits. Due to their territorial behavior and their diversity of parental care behaviors, Neotropical poison frogs are an excellent study system to investigate whether behavioral traits can influence sexual differentiation in intrinsic or extrinsic traits of individuals. Here, we evaluate whether territorial and parental care behaviors mediate sexual differentiation in ecological (habitat use) and phenotypic (coloration, morphology) traits in the critically endangered Lehmann’s poison frog (Oophaga lehmanni), a species in which males defend territories while females provide parental care. We found sex differences in habitat use and morphological traits, but not in coloration. Males use trunks and green leaves as perches more frequently and are found on higher substrates, than females. We found no sex differences in body size, but females have longer arms than males, which is probably associated with their parental duties (climbing trees to feed the tadpoles). Altogether, our results provide evidence that selection pressures act differently on male and female traits, and that territoriality and parental care may promote the evolution of sexual differentiation in dendrobatids. Long-term wildlife observations are essential to identify important life-history traits and to evaluate hypotheses about the behavioral ecology and conservation of this and other vertebrate species.
Scaling Interventions to Manage Chronic Disease: Innovative Methods at the Intersection of Health Policy Research and Implementation Science
Authors: McGinty Emma E. , Seewald Nicholas J. , Bandara Sachini , Cerdá Magdalena , Daumit Gail L. , Eisenberg Matthew D. , Griffin Beth Ann , Igusa Tak , Jackson John W. , Kennedy-Hendricks Alene , Marsteller Jill , Miech Edward J. , Purtle Jonathan , Schmid Ian , Schuler Megan S. , Yuan Christina T. , Stuart Elizabeth A. 
Published: September 2022

AbstractPolicy implementation is a key component of scaling effective chronic disease prevention and management interventions. Policy can support scale-up by mandating or incentivizing intervention adoption, but enacting a policy is only the first step. Fully implementing a policy designed to facilitate implementation of health interventions often requires a range of accompanying implementation structures, like health IT systems, and implementation strategies, like training. Decision makers need to know what policies can support intervention adoption and how to implement those policies, but to date research on policy implementation is limited and innovative methodological approaches are needed. In December 2021, the Johns Hopkins ALACRITY Center for Health and Longevity in Mental Illness and the Johns Hopkins Center for Mental Health and Addiction Policy convened a forum of research experts to discuss approaches for studying policy implementation. In this report, we summarize the ideas that came out of the forum. First, we describe a motivating example focused on an Affordable Care Act Medicaid health home waiver policy used by some US states to support scale-up of an evidence-based integrated care model shown in clinical trials to improve cardiovascular care for people with serious mental illness. Second, we define key policy implementation components including structures, strategies, and outcomes. Third, we provide an overview of descriptive, predictive and associational, and causal approaches that can be used to study policy implementation. We conclude with discussion of priorities for methodological innovations in policy implementation research, with three key areas identified by forum experts: effect modification methods for making causal inferences about how policies’ effects on outcomes vary based on implementation structures/strategies; causal mediation approaches for studying policy implementation mechanisms; and characterizing uncertainty in systems science models. We conclude with discussion of overarching methods considerations for studying policy implementation, including measurement of policy implementation, strategies for studying the role of context in policy implementation, and the importance of considering when establishing causality is the goal of policy implementation research.
Crossing borders, building new ones, or shifting boundaries? Shared narratives and individual paths towards inter/transdisciplinarity in research centres for urban sustainability
Authors: Norton Laura Soledad , Sonetti Giulia , Sarrica Mauro 
Published: September 2022

AbstractInter/transdisciplinarity (ITD) is a pillar of sustainability studies, often presented as the way to conduct research and practice especially in conflictual and politicised real-world stakeholder constellations. Several studies emphasise the need to consider the communicative processes through which it is put into practice. However, there is still a dearth of research that explores the meanings key actors associate with ITD and how they account for the material, practical and communicative facets of their everyday experience. This work seeks to collect the voice of leaders of inter/transdisciplinary research centres, identify shared repertoires used to interpret their experience in the field, and reflect on how shared narratives could inspire or impede researchers engaged in ITD. A discursive analysis was applied to 23 semi-structured interviews conducted with leaders of research centres on urban sustainability. Results identified diverse interpretative repertoires used to define ITD and to interpret the barriers that, in the eyes of these key actors, have to be crossed to become ITD researchers. These elements are combined into three main narratives used by participants to position themselves and the researchers involved in ITD. Despite being functional to self-representation, these shared narratives contribute towards depicting ITD as an individual escape, and interdisciplinary research centres as sanctuaries of a sort, thus paradoxically preserving the status quo. A third narrative advocates structural shifts and is coherent with the need for deeper changes and persistent recognition of ITD in sustainability studies.
Computational modeling method to estimate secondhand exposure potential from exhalations during e-vapor product use under various real-world scenarios
Authors: Edmiston Jeffery S. , Rostami Ali A. , Liang Qiwei , Miller Sandra , Sarkar Mohamadi A. 
Published: September 2022

AbstractPotential secondhand exposure of exhaled constituents from e-vapor product (EVP) use is a public health concern. We present a computational modeling method to predict air levels of exhaled constituents from EVP use. We measured select constituent levels in exhaled breath from adult e-vapor product users, then used a validated computational model to predict constituent levels under three scenarios (car, office, and restaurant) to estimate likely secondhand exposure to non-users. The model was based on physical/thermodynamic interactions between air, vapor, and particulate phase of the aerosol. Input variables included space setting, ventilation rate, total aerosol amount exhaled, and aerosol composition. Exhaled breath samples were analyzed after the use of four different e-liquids in a cartridge-based EVP. Nicotine, propylene glycol, glycerin, menthol, formaldehyde, acetaldehyde, and acrolein levels were measured and reported based on a linear mixed model for analysis of covariance. The ranges of nicotine, propylene glycol, glycerin, and formaldehyde in exhaled breath were 89.44–195.70 µg, 1199.7–3354.5 µg, 5366.8–6484.7 µg, and 0.25–0.34 µg, respectively. Acetaldehyde and acrolein were below detectable limits; thus, no estimated exposure to non-EVP users is reported. The model predicted that nicotine and formaldehyde exposure to non-users was substantially lower during EVPs use compared to cigarettes. The model also predicted that exposure to propylene glycol, glycerin, nicotine and formaldehyde among non-users was below permissible exposure limits.
NMR resonance assignment of the N-terminal GTPase domain of human Miro2 Bound to GTP
Authors: Smith Cassandra E. , Jones David N. M. 
Published: September 2022

AbstractMiro2 and Miro1 are mitochondrial-associated proteins critical for regulating mitochondrial movement within the cell. Both Miro1 and Miro2 have roles in promoting neuron function, but recently Miro2 has been shown to have additional roles in response to nutrient starvation in tumor cells. Miro1 and 2 consist of two small GTPase domains flanking a pair of EF-hands. The N-terminal GTPase (nGTPase) domain is responsible for initiating mitochondrial trafficking and interactions with GCN1 in prostate cancer. The crystal structure of Miro1 nGTPase bound to GTP has been solved. However, no structural data is available for the nGTPase domain of Miro2. To better understand the similarities and differences in the functions of Miro1 and Miro2, we have initiated structural studies of Miro2. Here we report the backbone NMR chemical shift assignments of a 22 KDa construct of the nGTPase domain of Miro2 bound to GTP that includes residues 1–180 of the full-length protein. We affirm that the overall secondary structure of this complex closely resembles that of Miro1 nGTPase bound to GTP. Minor variations in the overall structures can be attributed to crystal packing interactions in the structure of Miro1. These NMR studies will form the foundation for future work identifying the specific interaction sites between Miro2 and its cellular binding partners.