bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulated expression of INPP4B contributes to ovarian cancer growth and immune cell infiltration
Authors: Xu Jing , Wang Furong , Zhang Kaina , Li Yifeng , Ren Baoyin , Wang Jia , Gao Jingchun , Li Weiling 
Published: June 2022

Abstract
Background
Ovarian cancer is the most lethal gynecological cancer worldwide. Since the early clinical symptoms are not obvious, most patients with ovarian cancer are already in an advanced clinical stage when they are diagnosed. HGSOC accounts for the greatest number of ovarian cancer deaths and shows an association between an enhanced immune response and prolonged patient survival.
Methods
88 normal ovarian samples from TCGA and 149 ovarian cancer patients from GTEx were used to analyze gene expression, proteins and pathways associated with INPP4B in HGSOC, LGSOC and ovarian cancer. The correlation between INPP4B gene and immune factors was analyzed by TIMER website. The expression of INPP4B was verified by immunohistochemistry and Western Blotting. MTT assay, flow cytometry, and scratch assay were used to verify the effects of INPP4B on proliferation, apoptosis and migration. Tumor formation experiment to verify the effect of INPP4B on tumor formation.
Results
In this study, we performed data mining analysis of HGSOC and LGSOC samples. We first identified 25 tumor suppressor genes associated with poor prognosis and survival in HGSOC. Further immune infiltration analysis of these 25 genes with the TIMER web tool showed that among the 25 genes, INPP4B had the most pronounced effect on CD8 + T-cell infiltration. In addition, detection of INPP4B expression by IHC staining in clinical samples indicated that the INPP4B level was significantly lower in ovarian cancer tissues and negatively related to patient survival. Moreover, knockdown of INPP4B accelerated ovarian cancer cell growth and migration in vitro and vivo. Finally, the genes related to INPP4B in ovarian cancer were mainly enriched in the immune response and inflammatory response.
Conclusions
INPP4B plays an important role in cell proliferation, cell growth, cell migration, apoptosis and immune infiltration in OC. The INPP4B gene, as a potential tumor suppressor gene, may become a biomarker and therapeutic target for ovarian cancer.
A systematic review and meta-analysis on the aetiology and treatment patterns of thyrotoxicosis in Africa
Authors: Azeez Taoreed Adegoke , Lamidi Ayodeji Ogunremi , Osin Isaiah Olanrewaju 
Published: June 2022

Abstract
Background
Thyrotoxicosis is one of the most common endocrine disorders seen in clinical practice. This study aimed to determine the aetiologies and the treatment modalities of thyrotoxicosis in Africa.
Methods
The study design is a systematic review with a meta-analysis. Medical databases and the grey literature were systematically searched following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Studies done in Africa on the aetiology and treatment of thyrotoxicosis were selected. Meta-analysis was done with Meta XL an add-in software for Microsoft Excel using the DerSimonian Laird random effect. Study quality was determined with the Newcastle-Ottawa scale. Heterogeneity was determined using I2 statistic and the Cochran’s Q test.
Results
Thirty-four studies met the eligibility criteria. The total sample size was 3995. The studies were carried out between 1982 and 2021. The pooled mean age in the selected studies was 38.6 years. The pooled female: male ratio of the participants was 7:1. Graves’ disease, toxic multinodular goitre, toxic adenoma, thyroiditis and other rare causes accounts for about 54% (95%CI: 41–67), 39% (95%CI: 28–50), 10% (95%CI: 6–14), 2% (95%CI: 1–3), and 1% (95%CI: 0.1-2) of thyrotoxicosis cases seen in Africa respectively. Thionamides, thyroidectomy and radioiodine therapy were received by 95%, 17% and 11% of individuals with thyrotoxicosis respectively.
Discussion
The most common causes of thyrotoxicosis in Africa are Graves’ disease, toxic multinodular goitre, solitary toxic adenoma and thyroiditis. The most common treatment modality adopted in Africa is thionamides, followed by surgery and radioiodine therapy respectively.
Development of High Strength Lightweight Foamed Concrete with Low Cement Content
Authors: Khan M.Y. , Baqi A. , Sadique Md. R. , Khan Rizwan A. 
Published: June 2022

Abstract
Foamed concrete has versatile applications in the construction industry due to its lightweight nature and lower thermal conductivity. Compared to Autoclave Aerated Concrete (AAC), it also consumes low energy during its production. But, its weak structural behaviour restricted its applications in structural members. In the present study, the effect of a wide selection of filler on the compressive strength of foamed concrete has been studied. The present investigation shows that the lightweight concrete made with low cement content could achieve strength up to 15 MPa. Further, the effect of some Supplementary Cementitious Materials (SCMs) such as fly ash and silica fume on the tensile strength and shrinkage properties of foamed concrete has also been studied. Scanning electron microscopy (SEM) has been used to analyze the effect of different fillers on the properties of foamed concrete.
Threshold Effect of Economic Growth Rate on Persistence Suicide
Authors: Yang Tzu-Yi , Chen Ssu-Han , Lan Yu-Ting , Yang Yu-Tai 
Published: June 2022

Abstract
In this study, a Panel Smooth Transition Autoregression (PSTAR) model was introduced and the empirical results found nonlinear effects and heterogeneity of economic growth rate on the persistence of suicide rate, and can be summarized into three conclusions. First, the suicide rate has a persistent effect, and it varies over time depending on the transition variable within different threshold intervals. Second, the persistent effect will be manifested in different degrees by the change of the economic growth rate. With the increase of the lag period of the suicide rate, the effect of the influence will gradually decrease. Third, the marginal impact in the third year is much lower than the suicide rate in the first year.JEL Code: I12, I15, Q56
Development and psychometric properties of the Maternal Ambivalence Scale in Spanish women
Authors: Martín-Sánchez Magdalena Belén , Martínez-Borba Verónica , Catalá Patricia , Osma Jorge , Peñacoba-Puente Cecilia , Suso-Ribera Carlos 
Published: June 2022

Abstract
Background: maternal ambivalence, which refers to experiencing mixed emotions about motherhood, like happiness and sadness, is frequent during the perinatal period.Aim: Due to the relevance of this topic and the lack of psychometrically-sound instruments to measure it, this study aims to develop and test a measure of maternal ambivalence called Maternal Ambivalence Scale (MAS).Methods: participants were 1424 Spanish women who were either pregnant (33%) or recent mothers of children under 2 years (67%). They responded to the MAS and measures of anxiety and depressive symptoms and life satisfaction. Analyses included exploratory and confirmatory factor solutions for the MAS, as well as bivariate correlations to investigate sources of validity evidence. Comparisons between pregnant and postpartum women were also examined. Results: a three-factor solution (Doubts, Rejection, and Suppression) for the MAS showed a good model fit both in exploratory (Chi-square=274.6, p<.001, RMSEA=0.059, RMSEA 95% CI=[0.052, 0.066], CFI=0.985, TLI=0.974) and confirmatory analyses (Chi-square=428.0, p<.001, RMSEA=0.062, RMSEA 95% CI=[0.056, 0.068], CFI=0.977, TLI=0.971). Doubts, Rejection, and Suppression were associated with higher anxiety and depressive symptoms, as well as lower life satisfaction (all p<.001). Pregnant women presented greater Rejection (t=2.09, p=.037, 95% CI=[-0.58, -0.02]) and less Suppression (t=- 3.08, p=.002, 95% CI=[0.17, 0.77]) than mothers.Conclusions: the MAS might be a useful instrument to explore several components of maternal ambivalence, that is the presence of doubts and rejection about maternity, as well as coping styles used to deal with ambivalence. The scale might be useful for clinicians and researchers interested in maternal mental health.
Microbiome Evidence of Digestive Reflux and Inflammation in the Pancreatic Stump with Pancreatic Fistula After Pancreaticoduodenectomy
Authors: Wang Tiegong , Li Fengshan , Tian Liang , Xu Yanfeng , Zhang Lei , Zhao Xiulei , Kong Deshuai 
Published: June 2022

Abstract
Background
In the past century, postoperative pancreatic fistula (POPF) has been the major complication and cause of mortality after pancreaticoduodenectomy (PD). No one knows what exactly happens in the pancreatic stump after POPF. The hypothesis of this manuscript was to investigate changes that occur in the pancreatic stump.
Methods
Two patients who had previously undergone PD for malignant tumors subsequently underwent complete pancreatectomy for life-threatening abdominal bleeding in the context of POPF. The pancreatic stumps were pathologically analyzed. It’s hard to prove digestive reflux directly. But we found that we can use 16s rRNA gene sequencing to detect the distribution of bacteria in the jejunum, pancreatic head, and pancreatic stump.
Results
Inflammation is widespread in both patients' pancreatic stumps, including hematoma, edema, infiltration of inflammatory cells, scattered necrosis, and acinar-duct metaplasia (ADM). The whole pancreatic stump shows a proliferative state. Apoptosis decreases significantly or even disappears. The inflammation of the pancreatic stump shows a descending trend from the anastomosis to the tail. Simultaneously, we found a universal phenomenon: chemotaxis in the ductal system. Inflammatory cells penetrate the ducts formed by ADM and gather in the ducts. The bacteria from duodena are also discovered in the major part of the pancreatic stump. The bacterial distribution shows the same trend as inflammation.
Conclusions
Inflammation and ADM widely exist in the pancreatic stump with POPF after PD and show a descending trend from the anastomosis to the pancreatic tail. Digestive reflux through the pancreatic ductal system does happen during the pancreatic fistula. We could stop POPF if we eliminate the digestive reflux and reduce the inflammation in the pancreatic stump.
A Transparency Statement Transforms Community-Police Interactions
Authors: Yeager David , Dobson Kyle , Dittmann Andrea 
Published: June 2022

Abstract
Over the last three decades, billions of dollars have been invested in community-oriented policing approaches that are meant to promote positive interactions between officers and civilians. However, trust in law enforcement remains low. Our qualitative analysis of over 500 hours of naturalistic observations suggested this is because police officers can make civilians feel threatened even when they are not actively investigating a crime. Here we use a pre-registered field experiment (N = 232 community members interacting with police officers) to show that civilians are less threatened and report greater trust when officers add a 10-word “transparency statement” (“I'm walking around trying to get to know the community”) to the beginning of interactions communicating their benevolent intent. Corroboration of these conclusions comes from natural language processing analyses of the interaction transcripts and from ambulatory sensors that monitored community members’ sympathetic nervous system activation. Further, five online experiments isolated the conditions under which transparency statements were most impactful (total N = 2,241). This multi-method investigation highlights an under-appreciated reason why authority figures such as police so often fail to promote positive relationships with the community: a lack of transparency about the reasons for their behavior.
Assessment of native cadmium-tolerant bacteria in cacao (Theobroma cacao L.) - cultivated soils in Cundinamarca-Colombia
Authors: Cordoba-Novoa Henry A. , Cáceres-Zambrano Jeimmy , Torres-Rojas Esperanza 
Published: June 2022

Abstract
Traces of cadmium (Cd) have been reported in chocolate products due to growing cacao plants in soils with Cd and the ability of cacao plants to accumulate it. A strategy to minimize this uptake of Cd is by using cadmium-tolerant bacteria (CdtB) to sequester the Cd. This study was aimed to isolate and characterize CdtB associated with cacao-cultivated soils in Cundinamarca-Colombia. Thirty CdtB morphotypes were isolated and almost half of them showed the capacity to fix nitrogen, solubilize phosphates and degrade cellulose. Besides, all strains were selected for analysis of 16S rRNA sequence. The soil with the highest natural concentration of Cd (location Y3) showed higher abundance, unique morphotypes, and dominance of isolated CdtB. Pseudomona putida GB78, Pseundomona aeruginosa NB2, and Burkholderia sp. NB10 were the only morphotypes that grew on 18 up to 90 (GB78) and 140 mg Kg− 1 Cd (NB2-NB10). GB78 showed the highest Cd bioaccumulation (5.92 mg g− 1). Our results expand the knowledge about culturable CdtB in cacao-cultivated soils with the potential to develop soil management strategies to mitigate the uptake of Cd by cacao plants, thereby preventing the potential impact on human health, food safety, and the cacao economy.
ACAA2 was a predictor for the prognosis and immunotherapy response of hepatocellular carcinoma
Authors: Wu Dehai , Qi Xueting , Yao Yuanfei , Huang Lining , Liao Guanqun , Dong Bowen , Ma Yong , Yang Guangchao 
Published: June 2022

Abstract
Background
Immunotherapy is the first-line treatment for advanced hepatocellular carcinoma (HCC). However, immunotherapy benefits are limited in certain patients. Therefore, identifying effective biomarkers is important for the efficient use of immunotherapy. In the present study, acetyl-CoA acyltransferase 2 (ACAA2), which was decreased during HCC initiation, was identified as a gene of interest.
Methods
In this study, we aimed to explore the role of ACAA2 in the progression of HCC and the link between ACAA2 and tumor microenvironment. Bulk RNA data and single-cell RNA data were acquired from the Cancer Genome Atlas and Gene Expression Omnibus. Both in vitro and in vivo studies were used to determine the effect of ACAA2 on the progression of HCC, and RNA sequencing analysis was performed to explore the mechanism.
Results
The patient with low ACAA2 level had an immunosuppressive microenvironment in the HCC and could be poor response to immunotherapy. Decreased ACAA2 facilitated HCC proliferation and metastasis by activating the nuclear factor-κB (NFκB) signaling pathway. And increased CXCL1 induced by NFκB signaling pathway was responsible for low level of ACAA2 related immune suppression microenvironment. Furthermore, the expression of ACAA2 in immune cells, CD4+TCF7+T, CD4+FOXP3+T, CD8+GZMK+T, and CD8+KLRD1+T cells, was inversely correlated with the composition of CD8+PDCD1+T cells in HCC. This effect may be due to the CCL5-CCRs and HLA-E-KLRCs ligand-receptor networks.
Conclusions
ACAA2 was a favorable indicator for the prognosis of HCC and an ideal biomarker for immunotherapy.
Effect of Fingolimod Therapy on Quantitative Macular Changes among Patients with Relapsing-Remitting Multiple Sclerosis: A Four-Year Follow-Up Study from Oman
Authors: Al-Rashdi Aseel A. , Sabt Buthaina I. , Al-Mujaini Abdullah 
Published: June 2022

Abstract
Purpose: Fingolimod (FTY-720) is an immunomodulatory oral agent approved for the treatment of relapsing-remitting multiple sclerosis (RRMS); however, several clinical trials have shown that some recipients may develop macular oedema (ME) as an adverse reaction. As there are no studies assessing the long-term (>1 year) effect of fingolimod on the macula, this study aimed to evaluate the quantitative effect of fingolimod therapy on central macular thickness (CMT) and total macular volume (TMV) over a four-year period.Methods: This retrospective longitudinal cohort study was performed between January 2014 and December 2018. A total of 21 patients with RRMS receiving fingolimod therapy were recruited and followed-up over four years to assess CMT and TMV changes measured using spectral domain optical coherence tomography. A paired sample t-test was used to compare mean CMT and TMV values calculated at baseline prior to the initiation of fingolimod therapy with those observed at three, six, 12, 24, 36 and 48 months of treatment. Results: None of the patients developed ME over the four-year study period. In addition, there was no significant difference in baseline mean CMT values and those observed at a four-year follow-up. Although mean TMV values remained constant initially, there was a significant decrease towards the end of the study period. Conclusions: Long-term fingolimod therapy did not result in significant CFT changes. While there was a reduction in TMV towards the end of the study, this is likely due to the degenerative effect of the disease itself on the nerve fibres of the retina.
Will right versus left acute colonic diverticulitis location affect the likelihood of emergent operation?
Authors: Lu Tzung-Ju , Po-Cheng Lo , Chen Chuang-Wei , Hsiao Koung-Hung 
Published: June 2022

Abstract
Purpose
Modified Hinchey classification with computed tomography (CT)providesa good predictive factorfor medical failure. By using CT, the location of diverticulitis is proved as an important factor for medical treatment and right-sided diverticulitis is successfully treated medicallymore. However, will more accurate location for diverticulitis affect treatment is not evaluated.
Methods
Patients who were diagnosed withacute colonic diverticulitis by abdominopelvic CT were retrospectively recorded and analyzed.Patient demographics and clinical features were obtained from patients’ records.Abdominopelvic CT findings were categorized using the location in the colon, distribution of free air in the bowel lumen, fluid accumulation, and type of extra-luminal free air.
RESULTS
Patients with right-sided diverticulitis were more likely to be younger and elevation of C-reactive protein (CRP) in patients with left-sided diverticulitiswithout significantly different.Only 6.3% of patients received surgical treatment and left-sided diverticulitis is more suitablefor operation (P = 0.02).Most patients with local free air underwent successful medical treatment. The distribution of diverticulitis is different between right- and left-sided diverticulitis and lateral distribution included more cases of surgery.
CONCLUSIONS
Although there wasno statistical difference between location and emergent operation, we observed that location in the lateral region was an indicatorfor emergent operation. Right-and left-sided diverticulitis manifest indifferent locations, which maybe one of the causes of medical treatmentfailure. Asfeweremergent operations have been performed in recent years, we suggest conducting additionalstudies with larger cohortsto confirm the relationship between location and surgery.
Double ionisation of disulfur
Authors: Olsson Emelie , Ayari Tarek , Ideböhn Veronica , Wallner Måns , Squibb Richard J. , Andersson Jonas , Roos Andreas Hult , Stranges Stefano , Dyke John M. , Eland John H.D. , Hochlaf Majdi , Feifel Raimund 
Published: June 2022

Abstract
Using time-of-flight multiple electron and ion coincidence techniques in combination with a helium gas discharge lamp and synchrotron radiation, the double ionisation spectrum of disulfur (S2) and the subsequent fragmentation dynamics of its dication are investigated. The S2 sample was produced by heating mercury sulfide (HgS), whose vapour at a suitably chosen temperature consists primarily of two constituents: S2 and atomic Hg. A multi-particle-coincidence technique is thus particularly useful for retrieving spectra of S2 from ionisation of the mixed vapour. The results obtained are compared with detailed calculations of the electronic structure and potential energy curves of S2+2 which are also presented. These computations are carried out using configuration interaction methodology. The experimental results are interpreted with and strongly supported by the computational results.
Ecofriendly biosynthesis of Ag-NPs by Streptomyces griseus eradicating Candida albicans associated with different types of dysspermatism
Authors: El-Enain Inas M. Abou , Abed Nermine N. , Helal Eman E. , Abdelkhalek Eman S. , Suleiman Waleed 
Published: June 2022

Abstract
Silver nanoparticles Ag-NPs have potent biological properties such as antibacterial, antifungal, anti-viral and anti-inflammatory activities. A simple, eco-friendly, inexpensive method was conducted in order to synthesize silver nanoparticles by actinomycetes. A total of 17 actinomycetes isolates were recovered from serially diluted compost sample and subsequently examined for their capabilities to produce Ag-NPs. Alongside, Candida albicans was isolated from human semen that affect male infertility, and subsequently submitted to susceptibility test using the well diffusion method. It was found that the Ag-NPs biosynthesized by Streptomyces griseus Azhar.S2 have high zone of inhibition against Candida albicans which was recorded by measuring the diameter of inhibition zone that was 20.5 ± .05 mm. The biosynthesized Ag-NPs had been characterized using UV-vis spectroscopy, FTIR, EDX and TEM. Conclusively, Candida albicans isolated from different types of dysspermatism human semen was highly eradicated by microbially synthesized Ag-NPs from Streptomyces griseus Azhar.S2.
Group Contribution-based Graph Convolution Network: Pure Property Estimation Model
Authors: Hwang Sun Yoo , Kang Jeong Won 
Published: June 2022

Abstract
Properties data for chemical compounds are essential information for the design and operation of chemical processes. Experimental values are reported in the literature, but such are too scarce compared with exploding demand for data. When the data are not available, various estimation methods are employed. The group contribution method is one of the standards and simple techniques used today. However, these methods have inherent inaccuracy due to the simplified representation of the molecular structure. More advanced methods are emerging, including improved molecular representations and handling experimental data. However, such processes also suffer from a lack of valid data for adjusting many parameters. We suggest a compromise between a complex machine learning algorithm and a linear group contribution method in this contribution. Instead of representing a molecule using a graph of atoms, we employed bulkier blocks - a graph of functional groups. The new approach dramatically reduced the number of adjustable parameters for machine learning. The result shows higher accuracy than the conventional methods. The whole process was also examined in various aspects - incorporating uncertainties in the data, the robustness of the fitting process, and detecting outlier data.