bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field observations and remote assessment identify climate change, recreation, invasive species, and livestock as top threats to critically imperiled rare plants in Nevada
Authors: McClinton Jamey D. , Kulpa Sarah M. , Grames Eliza M. , Leger Elizabeth A. 
Published: December 2022

IntroductionRare plant species comprise &gt;36.5% of the world’s flora and disproportionately support ecosystem function and resilience. However, rare species also lead global plant extinctions, and unique ecological characteristics can make them vulnerable to anthropogenic pressure. Despite their vulnerability, many rare plants receive less monitoring than is needed to inform conservation efforts due to limited capacity for field surveys.MethodsWe used field observations and geospatial data to summarize how 128 imperiled, rare vascular plant species in Nevada are affected by various threats. We assessed correlations between threats predicted by geospatial data and threats observed on the ground and asked how historic and current threats compare.ResultsThe most commonly observed threats were from recreation, invasive and non-native/alien species, and livestock farming and ranching. Threat prevalence varied by elevation (e.g., a greater variety of threats at lower elevations, greater threat from climate change observed at higher elevations) and land management. There was a 28.1% overall correlation between predicted and observed threats, which was stronger for some threats (e.g., development of housing and urban areas, livestock farming and ranching) than others. All species experienced extreme climatic differences during 1990-2020 compared to baseline conditions, with the most extreme change in southern Nevada. The average number of threats observed per occurrence increased by 0.024 each decade.DiscussionWhile geospatial data did not perfectly predict observed threats, many of these occurrences have not been visited in over 30 years, and correlations may be stronger than we were able to detect here. Our approach can be used to help guide proactive monitoring, conservation, and research efforts for vulnerable species.
Development and validation of a novel risk model for predicting atrial fibrillation recurrence risk among paroxysmal atrial fibrillation patients after the first catheter ablation
Authors: Li Guangling , Wang Xiaomei , Han Jing-jing , Guo Xueya 
Published: December 2022

AimsSeveral models have been developed to predict the risk of atrial fibrillation (AF) recurrence after radiofrequency catheter ablation (RFCA). However, these models are of poor quality from the start. We, therefore, aimed to develop and validate a predictive model for post-operative recurrence of AF.Materials and methodsIn a study including 433 patients undergoing the first circumferential pulmonary vein isolation (CPVI) procedure, independent predictors of AF recurrence were retrospectively identified. Using the Cox regression of designated variables, a risk model was developed in a random sample of 70% of the patients (development cohort) and validated in the remaining (validation cohort) 30%. The accuracy and discriminative power of the predictive models were evaluated in both cohorts.ResultsDuring the established 12 months follow-up, 134 patients (31%) recurred. Six variables were identified in the model including age, coronary artery disease (CAD), heart failure (HF), hypertension, transient ischemic attack (TIA) or cerebrovascular accident (CVA), and left atrial diameter (LAD). The model showed good discriminative power in the development cohort, with an AUC of 0.77 (95% confidence interval [CI], 0.69–0.86). Furthermore, the model shows good agreement between actual and predicted probabilities in the calibration curve. The above results were confirmed in the validation cohort. Meanwhile, decision curve analysis (DCA) for this model also demonstrates the advantages of clinical application.ConclusionA simple risk model to predict AF recurrence after ablation was developed and validated, showing good discriminative power and calibration.
One-year results from the Assessing MICRO-vascular resistances via IMR to predict outcome in ST-elevation myocardial infarction patients with multivessel disease undergoing primary PCI (AMICRO) trial
Authors: Fineschi Massimo , Verna Edoardo , Barioli Alberto , Mezzapelle Giuseppe , Bartolini Davide , Turiano Giovanni , Guiducci Vincenzo , Manari Antonio , Lucarelli Katya , Uguccioni Lucia , Repetto Alessandra , Tarantini Giuseppe 
Published: December 2022

BackgroundIn ST-elevation myocardial infarction (STEMI) patients undergoing primary percutaneous coronary angioplasty (PPCI) the index of microcirculatory resistance (IMR) correlates to the extent of myocardial damage and left ventricular (LV) function recovery. Data on the IMR time-course and impact on clinical outcome in STEMI patients with multi-vessel disease (MVD) are scarce.AimsWe designed a prospective, multicenter clinical trial to assess the infarct-related artery (IRA)-IMR in STEMI patients with MVD undergoing PPCI and to explore its potential in relationship with outcome and LV remodeling.MethodsThe study enrolled 242 STEMI patients with MVD. Both fractional flow reserve (FFR) and IMR of the IRA were assessed after successful PPCI. Then, FFR/IMR measurements were repeated in the IRA at a staged angiography, and FFR-guided angioplasty was performed in non-IRA lesions. The primary endpoint was the composite of cardiovascular death, re-infarction, re-hospitalization for heart failure, resuscitation or appropriate ICD shock at 1-year follow-up.ResultsA significant improvement of IRA-IMR values (from 47.9 to 34.2, p &lt; 0.0001) was observed early after PPCI. Staged FFR-guided angioplasty was performed in 102 non-IRA lesions. We failed to find a correlation between IRA-IMR, clinical events and LV remodeling. Notwithstanding, in patients with anterior STEMI an inverse correlation between initial IMR values and LV function at follow-up was observed.ConclusionAfter successful PPCI, a significant proportion of patients with STEMI and MVD had coronary microvascular dysfunction as assessed by IMR that recovered early after reperfusion. Higher IMR values predicted lack of improvement of LV function only in anterior STEMI.Clinical trial registrationhttps://clinicaltrials.gov/, identifier [NCT 02325973].
The novel compound heterozygous rare variants may impact positively selected regions of TUBGCP6, a microcephaly associated gene
Authors: Chen Jianhai , Ying Lijuan , Zeng Li , Li Chunyu , Jia Yangying , Yang Hao , Yang Guang 
Published: December 2022

IntroductionThe microcephaly is a rare and severe disease probably under purifying selection due to the reduction of human brain-size. In contrast, the brain-size enlargement is most probably driven by positive selection, in light of this critical phenotypical innovation during primates and human evolution. Thus, microcephaly-related genes were extensively studied for signals of positive selection. However, whether the pathogenic variants of microcephaly-related genes could affect the regions of positive selection is still unclear.MethodsHere, we conducted whole genome sequencing (WGS) and positive selection analysis.ResultsWe identified novel compound heterozygous variants, p.Y613* and p.E1368K in TUBGCP6, related to microcephaly in a Chinese family. The genotyping and the sanger sequencing revealed the maternal and the paternal origin for the first and second variant, respectively. The p.Y613* occurred before the second and third domain of TUBGCP6 protein, while p.E1368K located within the linker region of the second and third domain. Interestingly, using multiple positive selection analyses, we revealed the potential impacts of these variants on the regions of positive selection of TUBGCP6. The truncating variant p.Y613* could lead to the deletions of two positively selected domains DUF5401 and Spc97_Spc98, while p.E1368K could impose a rare mutation burden on the linker region between these two domains.DiscussionOur investigation expands the list of candidate pathogenic variants of TUBGCP6 that may cause microcephaly. Moreover, the study provides insights into the potential pathogenic effects of variants that truncate or distribute within the positively selected regions.
Extent of male involvement and associated factors in antenatal care service utilization in Bench Sheko zone, Southwest Ethiopia: A community-based cross-sectional study
Authors: Mekonen Mengistu , Shifera Nigusie , Bogale Biruk , Assefa Ashenafi 
Published: December 2022

BackgroundIn many low- and middle-income countries (LMIC), men are the key decision-makers and chief providers, often determining women's access to economic resources and maternal health services. Despite the important role of men in maternal healthcare, the involvement of male partners in maternal and child health in LMIC, including Ethiopia, is low.ObjectivesThis study aims to assess the extent of male involvement and associated factors in antenatal care (ANC) service utilization in the Bench Sheko zone, Southwest, Ethiopia.MethodsA community-based, cross-sectional study was conducted in the Bench Sheko zone from February to May 2021. A multistage sampling technique was employed to select study participants. Variables with p-values &lt;0.25 in binary logistic regression were selected as candidates for multiple logistic regression to determine independent factors associated with male involvement adjusting for sociodemographic, health service, personal, environmental, and knowledge-related factors.ResultsA total of 816 men took part in the study, with a 98.2% response rate. The prevalence of male involvement in ANC utilization was 38.2%. In multivariate analyses, residence (urban), age (25–34), education (diploma and above), income (≥2,500 Ethiopian Birr, ETB), and high knowledge of the advantages of ANC were shown to be positively associated with male involvement in ANC utilization.ConclusionMale involvement in ANC utilization was low. Considering the importance of male involvement in maternal healthcare, we advocate for policies and strategies that can improve knowledge of the advantages of ANC among men and can enhance their engagement in maternal care. Special attention should be given to younger partners and those partners who live in rural areas.
Bidirectional two-sample Mendelian randomization study of causality between rheumatoid arthritis and myocardial infarction
Authors: Guo Hao-Yang , Wang Wei , Peng Hui , Yuan Hui 
Published: December 2022

BackgroundEpidemiological evidence suggests an association between rheumatoid arthritis (RA) and myocardial infarction (MI). However, causality remains uncertain. Therefore, this study aimed to explore the causal association between RA and MI.MethodsUsing publicly available genome-wide association study summary datasets, bidirectional two-sample Mendelian randomization (TSMR) was performed using inverse-variance weighted (IVW), weighted median, MR-Egger regression, simple mode, and weighted mode methods.ResultsThe MR results for the causal effect of RA on MI (IVW, odds ratio [OR] = 1.041, 95% confidence interval [CI]: 1.007–1.076, P = 0.017; weighted median, OR = 1.027, 95% CI: 1.006–1.049, P = 0.012) supported a causal association between genetic susceptibility to RA and an increased risk of MI. MR results for the causal effect of MI on RA (IVW, OR = 1.012, 95% CI: 0.807–1.268, P = 0.921; weighted median, OR = 1.069, 95% CI: 0.855–1.338, P = 0.556) indicated that there was no causal association between genetic susceptibility to MI and an increased risk of RA.ConclusionBidirectional TSMR analysis supports a causal association between genetic susceptibility to RA and an increased risk of MI but does not support a causal association between genetic susceptibility to MI and an increased risk of RA.
Autoimmunity in monogenic combined immune deficiencies with associated or syndromic features
Authors: Sharifinejad Niusha , Azizi Gholamreza , Chavoshzadeh Zahra , Mahdaviani Seyed Alireza , Alan Mahnaz Seifi , Tavakol Marzieh , Sadri Homa , Nabavi Mohammad , Ebrahimi Sareh Sadat , Shirkani Afshin , Vosughi Motlagh Ahmad , Safarirad Molood , Aghamahdi Fatemeh , Nazari Farzad , Delavari Samaneh , Jamee Mahnaz , Fayyaz Farimah , Samimisedeh Parham , Matani Rahman , Esmaeili Marzie , Yazdani Reza , Rezaei Nima , Abolhassani Hassan 
Published: December 2022

BackgroundCombined immune deficiencies (CIDs) with associated or syndromic features are a highly heterogeneous subgroup of inherited immune disorders. These patients represent specific clinical complications with an increased risk of autoimmune conditions.MethodsWe analyzed data of monogenic patients with syndromic CIDs adopted from the Iranian inborn errors of immunity registry up to January 2022. A comprehensive comparison in terms of demographic, clinical, and immunological features was performed between patients with and without autoimmunity and also among four mutation groups with the most registered cases including ATM, STAT3 (AD-LOF), DNMT3B/ZBTB24, and WAS mutations.ResultsA total of 137 patients with monogenic syndromic CIDs were included. Most commonly mutated genes were the ATM [80 (58.4%)] and STAT3 (AD-LOF) [19 (13.9%)], followed by DNMT3B [11 (8%)], and WAS [11 (8%)]. More than 18% of all patients with syndromic CIDs, including most DNMT3B/ZBTB24 mutations patients, were clinically diagnosed with antibody deficiencies before genetic evaluation. Patients with ATM and WAS mutations had the latest age of onset and the lowest age of diagnosis, respectively. Autoimmune disorders were diagnosed in 24 patients at a median age of 3.5 (2.6-6.0) years, 70.6% of which were diagnosed prior to the diagnosis of immunodeficiency. Lymphoproliferation, particularly hepatosplenomegaly, was significantly higher in patients with autoimmunity (p=0.004). Syndromic CID patients with autoimmunity had significantly lower IgG levels. Hematologic autoimmunity mainly immune thrombocytopenic purpura was the most frequent autoimmunity among major groups of ATM, STAT3 (AD-LOF), DNMT3B/ZBTB24, and WAS mutations, however ATM-mutated patients present more diversified involved organs including rheumatologic, gastrointestinal and dermatologic autoimmunity.ConclusionAbout 18% of patients with monogenic syndromic CIDs developed autoimmunity, mainly in the form of hematological immune diseases. Autoimmunity could be an early-onset involvement with a potential diagnostic impact on suspicious cases of syndromic CIDs.
Positive effect of Bifidobacterium animalis subsp. lactis VHProbi YB11 in improving gastrointestinal movement of mice having constipation
Authors: Cui Hongchang , Wang Qian , Feng Congrui , Guo Chaoqun , Zhang Jingyan , Bu Xinping , Duan Zhi 
Published: December 2022

IntroductionThe aim of this study was to investigate the effects of Bifidobacterium animalis subsp. lactis VHProbi® YB11 (YB11) on attenuating sucralfate-induced constipation in BALB/c mice. The strain of YB11 exhibited favorable tolerance of simulated gastrointestinal (GI) juice. Only 0.42 Log value declined when the live cells of YB11 were co-incubated with simulated GI juice. Meanwhile, this strain also displayed perfect ability to adhere the intestinal epithelium Caco-2 cells with adhesion index of 18.5. 24 of female mice were randomized into four groups.MethodsThe normal group (NOR) was fed with a normal diet, whereas the placebo group (PLA), positive group (POS), and probiotic group (PRO) were fed with sucralfate to induce constipation. After first successfully establishing the constipation model, groups NOR and PLA received the oral administration of saline solutions. Meanwhile, the POS and PRO groups were orally administered phenolphthalein and YB11 suspensions, respectively. Several indices, including fecal water content, GI transit time, short-chain fatty acids (SCFAs), intestinal neuropeptides level, and histopathology of colonic tissues, were investigated. Results and DiscussionCompared with PLA, YB11 had a positive effect in increasing the fecal water content and intestinal peristalsis. Some positive trends, including the acetic and total acids level of fecal samples, and the colonic tissue histopathology, were also observed. Furthermore, YB11 had an ability to upregulate the levels of gut excitatory neuropeptides including motilin, gastrin, and substance P, whereas it downregulated the levels of inhibitory neuropeptides including endothelin-1, somatostatin, and vasoactive intestinal peptide. We conclude that the strain YB11 has a positive impact on improving gastrointestinal mobility and reducing the severity of constipation.
Prevalence of malaria resistance-associated mutations in Plasmodium falciparum circulating in 2017–2018, Bo, Sierra Leone
Authors: Leski Tomasz A. , Taitt Chris Rowe , Colston Sophie M. , Bangura Umaru , Holtz Andrew , Yasuda Chadwick Y. , Reynolds Nathanael D. , Lahai Joseph , Lamin Joseph M. , Baio Victoria , Ansumana Rashid , Stenger David A. , Vora Gary J. 
Published: December 2022

IntroductionIn spite of promising medical, sociological, and engineering strategies and interventions to reduce the burden of disease, malaria remains a source of significant morbidity and mortality, especially among children in sub-Saharan Africa. In particular, progress in the development and administration of chemotherapeutic agents is threatened by evolved resistance to most of the antimalarials currently in use, including artemisinins.MethodsThis study analyzed the prevalence of mutations associated with antimalarial resistance in Plasmodium falciparum from 95 clinical samples collected from individuals with clinically confirmed malaria at a hospital in Bo, Sierra Leone between May 2017 and December 2018. The combination of polymerase chain reaction amplification and subsequent high throughput DNA sequencing was used to determine the presence of resistance-associated mutations in five P. falciparum genes – pfcrt, pfmdr1, pfdhfr, pfdhps and pfkelch13. The geographic origin of parasites was assigned using mitochondrial sequences.ResultsRelevant mutations were detected in the pfcrt (22%), pfmdr1 (&gt;58%), pfdhfr (100%) and pfdhps (&gt;80%) genes while no resistance-associated mutations were found in the pfkelch13 gene. The mitochondrial barcodes were consistent with a West African parasite origin with one exception indicating an isolate imported from East Africa.DiscussionDetection of the pfmdr1 NFSND haplotype in 50% of the samples indicated the increasing prevalence of strains with elevated tolerance to artemeter + lumefantrine (AL) threatening the combination currently used to treat uncomplicated malaria in Sierra Leone. The frequency of mutations linked to resistance to antifolates suggests widespread resistance to the drug combination used for intermittent preventive treatment during pregnancy.
Influence of dietary n-3 long-chain fatty acids on microbial diversity and composition of sows’ feces, colostrum, milk, and suckling piglets’ feces
Authors: Llauradó-Calero Eudald , Climent Eric , Chenoll Empar , Ballester Maria , Badiola Ignacio , Lizardo Rosil , Torrallardona David , Esteve-Garcia Enric , Tous Núria 
Published: December 2022

IntroductionVery little is known about the impact of n-3 long-chain fatty acids (n-3 LCFAs) on the microbiota of sows and their piglets. The aim of this study was to evaluate the effect of n-3 LCFA in sow diets on the microbiota composition of sows’ feces, colostrum, and milk as well as that of piglets’ feces.MethodsTwenty-two sows were randomly assigned to either a control or an n-3 LCFA diet from service to weaning. Sows’ and piglets’ performance was monitored. The gestating and lactating sows’ microbiomes in feces, colostrum, and milk were characterized by 16s ribosomal RNA gene sequencing. The fecal microbiome from the two lowest (&gt;800 g) and the two highest birth weight piglets per litter was also characterized, and the LPS levels in plasma were analyzed at weaning.Results and Discussionn-3 LCFA increased microbiota alpha diversity in suckling piglets’ and gestating sows’ feces. However, no effects were observed in colostrum, milk, or lactating sows’ feces. Dietary n-3 LCFA modified the microbiota composition of gestating sows’ feces, milk, and suckling piglets’ feces, without affecting lactating sows’ feces or colostrum. In gestating sows’ feces and milk, the decrease in genus Succinivibrio and the increase of Proteobacteria phylum, due to the increased genera Brenneria and Escherichia, respectively, stand out. In the feces of suckling piglets, the higher abundance of the beneficial genus Akkermansia and Bacteroides, and different species of Lactobacillus are highlighted. In addition, positive correlations for families and genera were found between lactating sows’ feces and milk, milk and suckling piglets’ feces, and lactating sows’ feces and suckling piglets’ feces. To conclude, dietary n-3 LCFA had a positive impact on the microbiome of suckling piglet’s feces by increasing microbial diversity and some beneficial bacteria populations, had a few minor modifications on the microbiome of milk and gestating sows’ feces and did not change the microbiome in lactating sows’ feces or colostrum. Therefore, this study shows the effect of dietary n-3 LCFA on the microbiota of sows, colostrum, milk, and suckling piglets during the lactation period providing crucial information on the microbiota status at the early stages of life, which have an impact on the post-weaning.
Application of the robot-assisted implantation in deep brain stimulation
Authors: Ma Fang-Zhou , Liu De-Feng , Yang An-Chao , Zhang Kai , Meng Fan-Gang , Zhang Jian-Guo , Liu Huan-Guang 
Published: December 2022

IntroductionThis work aims to assess the accuracy of robotic assistance guided by a videometric tracker in deep brain stimulation (DBS).MethodsWe retrospectively reviewed a total of 30 DBS electrode implantations, assisted by the Remebot robotic system, with a novel frameless videometric registration workflow. Then we selected 30 PD patients who used stereotactic frame surgery to implant electrodes during the same period. For each electrode, accuracy was assessed using radial and axial error.ResultsThe average radial error of the robot-assisted electrode implantation was 1.28 ± 0.36 mm, and the average axial error was 1.20 ± 0.40 mm. No deaths or associated hemorrhages, infections or poor incision healing occurred.ConclusionRobot-assisted implantation guided by a videometric tracker is accurate and safe.
Multicenter, prospective feasibility study of Nano-Pulse Stimulation™ technology for the treatment of both nodular and superficial low-risk basal cell carcinoma
Authors: Ross Amy S. , Schlesinger Todd , Harmon Christopher B. , Moy Ronald L. , Rohrer Thomas E. , Mehregan Darius R. , Nuccitelli Richard , Johnston Lauren Jauregui , Knape William A. 
Published: December 2022

BackgroundNano-Pulse Stimulation™ (NPS™) therapy is a new, non-thermal bioelectric modality that applies ultrashort pulses of electric energy to trigger regulated cell death (RCD) in treated tissues. Instead of initiating necrosis by heating or freezing, NPS therapy permeabilizes intracellular organelles to activate the cell’s own self-destruct pathway of programmed or regulated cell death. Unlike cryotherapeutic procedures that can both damage structural tissues and diffuse into the periphery beyond the margins of the lesion, NPS therapy only affects cells within the treated zone leaving surrounding tissue and acellular components unaffected.MethodsIn this study we treated 37 basal cell carcinoma lesions on 30 subjects (NCT04918381). The treated lesions were photographed on 3-, 7-, 14-, 30- and 60-days after treatment. All subjects then underwent surgical excision for histological examination of the treated tissue.Results92% of the BCC lesions (34 of 37) showed complete histological clearance of BCC. Histologic analysis of the 3 cases where residual BCC was noted indicated that full energy coverage was not achieved, which could be remedied with an improved treatment guide to standardize and optimize the CellFX® procedure based on NPS technology.ConclusionThe CellFX procedure was shown to be safe and effective for the treatment of low-risk nodular and superficial BCC lesions.