bluuebunny/crossref_metadata_2025_split huggingface dataset:
First computational design using lambda-superstrings and in vivo validation of SARS-CoV-2 vaccine
Authors: Martínez Luis , Malaina Iker , Salcines-Cuevas David , Terán-Navarro Héctor , Zeoli Andrea , Alonso Santos , M. De la Fuente Ildefonso , Gonzalez-Lopez Elena , Ocejo-Vinyals J. Gonzalo , Gozalo-Margüello Mónica , Calvo-Montes Jorge , Alvarez-Dominguez Carmen 
Published: April 2022

AbstractCoronavirus disease 2019 (COVID-19) is the greatest threat to global health at the present time, and considerable public and private effort is being devoted to fighting this recently emerged disease. Despite the undoubted advances in the development of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, uncertainty remains about their future efficacy and the duration of the immunity induced. It is therefore prudent to continue designing and testing vaccines against this pathogen. In this article we computationally designed two candidate vaccines, one monopeptide and one multipeptide, using a technique involving optimizing lambda-superstrings, which was introduced and developed by our research group. We tested the monopeptide vaccine, thus establishing a proof of concept for the validity of the technique. We synthesized a peptide of 22 amino acids in length, corresponding to one of the candidate vaccines, and prepared a dendritic cell (DC) vaccine vector loaded with the 22 amino acids SARS-CoV-2 peptide (positions 50-71) contained in the NTD domain (DC-CoVPSA) of the Spike protein. Next, we tested the immunogenicity, the type of immune response elicited, and the cytokine profile induced by the vaccine, using a non-related bacterial peptide as negative control. Our results indicated that the CoVPSA peptide of the Spike protein elicits noticeable immunogenicity in vivo using a DC vaccine vector and remarkable cellular and humoral immune responses. This DC vaccine vector loaded with the NTD peptide of the Spike protein elicited a predominant Th1-Th17 cytokine profile, indicative of an effective anti-viral response. Finally, we performed a proof of concept experiment in humans that included the following groups: asymptomatic non-active COVID-19 patients, vaccinated volunteers, and control donors that tested negative for SARS-CoV-2. The positive control was the current receptor binding domain epitope of COVID-19 RNA-vaccines. We successfully developed a vaccine candidate technique involving optimizing lambda-superstrings and provided proof of concept in human subjects. We conclude that it is a valid method to decipher the best epitopes of the Spike protein of SARS-CoV-2 to prepare peptide-based vaccines for different vector platforms, including DC vaccines.
Age-related changes to vestibular heave and pitch perception and associations with postural control
Authors: Gabriel Grace A. , Harris Laurence R. , Gnanasegaram Joshua J. , Cushing Sharon L. , Gordon Karen A. , Haycock Bruce C. , Campos Jennifer L. 
Published: April 2022

AbstractFalls are a common cause of injury in older adults (OAs), and age-related declines across the sensory systems are associated with increased falls risk. The vestibular system is particularly important for maintaining balance and supporting safe mobility, and aging has been associated with declines in vestibular end-organ functioning. However, few studies have examined potential age-related differences in vestibular perceptual sensitivities or their association with postural stability. Here we used an adaptive-staircase procedure to measure detection and discrimination thresholds in 19 healthy OAs and 18 healthy younger adults (YAs), by presenting participants with passive heave (linear up-and-down translations) and pitch (forward–backward tilt rotations) movements on a motion-platform in the dark. We also examined participants’ postural stability under various standing-balance conditions. Associations among these postural measures and vestibular perceptual thresholds were further examined. Ultimately, OAs showed larger heave and pitch detection thresholds compared to YAs, and larger perceptual thresholds were associated with greater postural sway, but only in OAs. Overall, these results suggest that vestibular perceptual sensitivity declines with older age and that such declines are associated with poorer postural stability. Future studies could consider the potential applicability of these results in the development of screening tools for falls prevention in OAs.
Decomposing rural–urban differences in successful aging among older Indian adults
Authors: Muhammad T. , Srivastava Shobhit , Hossain Babul , Paul Ronak , Sekher T. V. 
Published: April 2022

AbstractThe modernization and shift towards urbanized lifestyles have triggered several diseases, and the context of aging varies in urban and rural settings in India. The study aimed to investigate the urban–rural differences in successful ageing among older adults in India and the contributing factors in those differences. The study utilizes data from nationally representative Longitudinal Ageing Study in India (LASI, 2017–18). The analytical sample size for the study was 31,464 older adults aged 60 years and above. Descriptive statistics and bivariate analysis were carried out to present the initial results. Multivariable logistic regression and decomposition analysis was used to find the associations between explanatory variables and successful aging and to identify the contributions of covariates that explain the rural–urban differences in successful ageing. A proportion of 32% and 24% of older adults from rural and urban areas were successful agers with an urban disadvantage. Urban-dwelling older adults had 0.67 times [95% confidence interval (CI): (0.64, 0.71)] lower unadjusted odds of successful ageing than rural older adults. Again, after adjusting for the effect of other explanatory variables, urban older adults had 0.92 times [CI: (0.87, 0.98)] lower odds of being successful agers than their rural counterparts. The major contributors to the rural–urban inequality in successful aging were differences in regional distribution (17% contribution), waist circumference (16%), working status (16%), body mass index (13%) and physical activity (8%) among rural and urban older adults. The urban disadvantage in aging successfully may reflect the higher prevalence of adverse lifestyle behaviours in urban dwellers and under-diagnosis and under-reporting of many diseases in rural areas, particularly non-communicable diseases, suggesting the need for further investigation.
Investigating mental workload-induced changes in cortical oxygenation and frontal theta activity during simulated flights
Authors: Hamann Anneke , Carstengerdes Nils 
Published: April 2022

AbstractMonitoring pilots’ cognitive states becomes increasingly important in aviation. Physiological measurement can detect increased mental workload (MWL) even before performance declines. Yet, changes in MWL are rarely varied systematically and few studies control for confounding effects of other cognitive states. The present study targets these shortcomings by analysing the effects of stepwise increased MWL on cortical activation, while controlling for mental fatigue (MF). 35 participants conducted a simulated flight with an incorporated adapted n-back and monitoring task. We recorded cortical activation with concurrent EEG and fNIRS measurement, performance, self-reported MWL and MF. Our results show the successful manipulation of MWL without confounding effects of MF. Higher task difficulty elicited higher subjective MWL ratings, performance decline, higher frontal theta activity and reduced frontal deoxyhaemoglobin (Hbr) concentration. Using both EEG and fNIRS, we could discriminate all induced MWL levels. fNIRS was more sensitive to tasks with low difficulty, and EEG to tasks with high difficulty. Our findings further suggest a plateau effect for high MWL that could present an upper boundary to individual cognitive capacity. Our results highlight the benefits of physiological measurement in aviation, both for assessment of cognitive states and as a data source for adaptive assistance systems.
Rapid evolution of SARS-CoV-2 challenges human defenses
Authors: Duarte Carlos M. , Ketcheson David I. , Eguíluz Víctor M. , Agustí Susana , Fernández-Gracia Juan , Jamil Tahira , Laiolo Elisa , Gojobori Takashi , Alam Intikhab 
Published: April 2022

AbstractThe race between pathogens and their hosts is a major evolutionary driver, where both reshuffle their genomes to overcome and reorganize the defenses for infection, respectively. Evolutionary theory helps formulate predictions on the future evolutionary dynamics of SARS-CoV-2, which can be monitored through unprecedented real-time tracking of SARS-CoV-2 population genomics at the global scale. Here we quantify the accelerating evolution of SARS-CoV-2 by tracking the SARS-CoV-2 mutation globally, with a focus on the Receptor Binding Domain (RBD) of the spike protein determining infection success. We estimate that the > 820 million people that had been infected by October 5, 2021, produced up to 1021 copies of the virus, with 12 new effective RBD variants appearing, on average, daily. Doubling of the number of RBD variants every 89 days, followed by selection of the most infective variants challenges our defenses and calls for a shift to anticipatory, rather than reactive tactics involving collaborative global sequencing and vaccination.
Prediction of abdominal CT body composition parameters by thoracic measurements as a new approach to detect sarcopenia in a COVID-19 cohort
Authors: Molwitz I. , Ozga A. K. , Gerdes L. , Ungerer A. , Köhler D. , Ristow I. , Leiderer M. , Adam G. , Yamamura J. 
Published: April 2022

AbstractAs most COVID-19 patients only receive thoracic CT scans, but body composition, which is relevant to detect sarcopenia, is determined in abdominal scans, this study aimed to investigate the relationship between thoracic and abdominal CT body composition parameters in a cohort of COVID-19 patients. This retrospective study included n = 46 SARS-CoV-2-positive patients who received CT scans of the thorax and abdomen due to severe disease progression. The subcutaneous fat area (SF), the skeletal muscle area (SMA), and the muscle radiodensity attenuation (MRA) were measured at the level of the twelfth thoracic (T12) and the third lumbar (L3) vertebra. Necessity of invasive mechanical ventilation (IMV), length of stay, or time to death (TTD) were noted. For statistics correlation, multivariable linear, logistic, and Cox regression analyses were employed. Correlation was excellent for the SF (r = 0.96) between T12 and L3, and good for the respective SMA (r = 0.80) and MRA (r = 0.82) values. With adjustment (adj.) for sex, age, and body-mass-index the variability of SF (adj. r2 = 0.93; adj. mean difference = 1.24 [95% confidence interval (95% CI) 1.02–1.45]), of the SMA (adj. r2 = 0.76; 2.59 [95% CI 1.92–3.26]), and of the MRA (adj. r2 = 0.67; 0.67 [95% CI 0.45–0.88]) at L3 was well explained by the respective values at T12. There was no relevant influence of the SF, MRA, or SMA on the clinical outcome. If only thoracic CT scans are available, CT body composition values at T12 can be used to predict abdominal fat and muscle parameters, by which sarcopenia and obesity can be assessed.
Factors influencing wind turbine avoidance behaviour of a migrating soaring bird
Authors: Santos Carlos D. , Ramesh Hariprasath , Ferraz Rafael , Franco Aldina M. A. , Wikelski Martin 
Published: April 2022

AbstractWind energy production has expanded as an alternative to carbon emitting fossil fuels, but is causing impacts on wildlife that need to be addressed. Soaring birds show concerning rates of collision with turbine rotor blades and losses of critical habitat. However, how these birds interact with wind turbines is poorly understood. We analyzed high-frequency GPS tracking data of 126 black kites (Milvus migrans) moving near wind turbines to identify behavioural mechanisms of turbine avoidance and their interaction with environmental variables. Birds flying within 1000 m from turbines and below the height of rotor blades were less likely to be oriented towards turbines than expected by chance, this pattern being more striking at distances less than 750 m. Within the range of 750 m, birds showed stronger avoidance when pushed by the wind in the direction of the turbines. Birds flying above the turbines did not change flight directions with turbine proximity. Sex and age of birds, uplift conditions and turbine height, showed no effect on flight directions although these factors have been pointed as important drivers of turbine collision by soaring birds. Our findings suggest that migrating black kites recognize the presence of wind turbines and behave in a way to avoid then. This may explain why this species presents lower collision rates with wind turbines than other soaring birds. Future studies should clarify if turbine avoidance behaviour is common to other soaring birds, particularly those that are facing high fatality rates due to collision.
Relationship between different serum cartilage biomarkers in the acute response to running and jumping in healthy male individuals
Authors: Dreiner Maren , Munk Tobias , Zaucke Frank , Liphardt Anna-Maria , Niehoff Anja 
Published: April 2022

AbstractThe effect of physical activity on serum cartilage biomarkers is largely unknown. The purpose of the study was to systematically analyze the acute effect of two frequently used exercise interventions (running and jumping) on the correlation of seven serum biomarkers that reflect cartilage extracellular matrix metabolism. Fifteen healthy male volunteers (26 ± 4 years, 181 ± 4 cm, 77 ± 6 kg) participated in the repeated measurement study. In session 1, the participants accomplished 15 × 15 series of reactive jumps within 30 min. In session 2, they ran on a treadmill (2.2 m/s) for 30 min. Before and after both exercise protocols, four blood samples were drawn separated by 30 min intervals. Serum concentrations of seven biomarkers were determined: COMP, MMP-3, MMP-9, YKL-40, resistin, Coll2-1 and Coll2-1 NO2. All biomarkers demonstrated an acute response to mechanical loading. Both the COMP and MMP-3 responses were significantly (p = 0.040 and p = 0.007) different between running and jumping (COMP: jumping + 31%, running + 37%; MMP-3: jumping + 14%, running + 78%). Resistin increased only significantly (p < 0.001) after running, and Coll2-1 NO2 increased significantly (p = 0.001) only after jumping. Significant correlations between the biomarkers were detected. The relationships between individual serum biomarker concentrations may reflect the complex interactions between degrading enzymes and their substrates in ECM homeostasis.
Bone mineral density alteration in obstructive sleep apnea by derived computed tomography screening
Authors: Daniel Sharon , Cohen-Freud Yafit , Shelef Ilan , Tarasiuk Ariel 
Published: April 2022

AbstractThe association between obstructive sleep apnea (OSA) and bone mineral density (BMD) is poorly elucidated and has contradictory findings. Abdominal computed tomography (CT) for other indications can provide a valuable opportunity for osteoporosis screening. Thus, we retrospectively explored the association between OSA and BMD by examining abdominal CT vertebrae images for a multitude of conditions and indications. We included 315 subjects (174 with OSA and 141 without OSA) who performed at least two CT scans (under similar settings). Both groups had a similar duration between the first and second CT scans of 3.6 years. BMD decreased in those with OSA and increased age. A multivariate linear regression indicated that OSA is associated with BMD alterations after controlling for age, gender, and cardiovascular diseases. Here, we report that OSA is associated with BMD alterations. Further studies are required to untangle the complex affect of OSA on BMD and the possible clinical implications of vertebra-depressed or femoral neck fractures.
GaN-based mini-LED matrix applied to multi-functional forward lighting
Authors: Nguyen Quang-Khoi , Lin Yi-Jou , Sun Ching , Lee Xuan-Hao , Lin Shih-Kang , Wu Chi-Shou , Yang Tsung-Hsun , Wu Tian-Li , Lee Tsung-Xian , Chien Chao-Hsin , Yu Yeh-Wei , Sun Ching-Cherng 
Published: April 2022

AbstractIn this paper, we propose and demonstrate to use of a single reflector with 68 segments to project vehicle low beam and high beam with the use of a GaN-based mini-LED matrix, which is a 5 × 6 LED die array. The design of the reflector is based on light field technology in considering etendue from the light source across the segments. The group of the segments with smaller etendue from the LED dies in the bottom 2 rows are used to project low beams. When the other LED dies are turned on, the reflector will project light upward and form the high beam. The selection of the turn-on LED dies in the mini-LED matrix can adjust the width of the illumination pattern so that an adaptive low/high beam can be performed. Besides, to extend the functionality of the headlamp module, we propose to dispense IR phosphor on LED dies in the high-beam zone of the GaN-based mini-LED matrix. Thus the vehicle can emit IR high beam, which can be imaged through a camera and can be incorporated with machine vision for an autonomous vehicle without using a complicated adaptive headlight to avoid glare. The proposed multi-function in spatial and spectral domains will be helpful to various applications with use of a mini-LED matrix.
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli
Authors: Abichabki Nathália , Zacharias Luísa V. , Moreira Natália C. , Bellissimo-Rodrigues Fernando , Moreira Fernanda L. , Benzi Jhohann R. L. , Ogasawara Tânia M. C. , Ferreira Joseane C. , Ribeiro Camila M. , Pavan Fernando R. , Pereira Leonardo R. L. , Brancini Guilherme T. P. , Braga Gilberto Ú. L. , Zuardi Antonio W. , Hallak Jaime E. C. , Crippa José A. S. , Lanchote Vera L. , Cantón Rafael , Darini Ana Lúcia C. , Andrade Leonardo N. 
Published: April 2022

AbstractThis study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising.
Remarkable diversity of vomeronasal type 2 receptor (OlfC) genes of basal ray-finned fish and its evolutionary trajectory in jawed vertebrates
Authors: Zhang Zicong , Sakuma Atsuhiro , Kuraku Shigehiro , Nikaido Masato 
Published: April 2022

AbstractThe vomeronasal type 2 receptor (V2R, also called OlfC) multigene family is found in a broad range of jawed vertebrates from cartilaginous fish to tetrapods. V2Rs encode receptors for food-related amino acids in teleost fish, whereas for peptide pheromones in mammals. In addition, V2Rs of teleost fish are phylogenetically distinct from those of tetrapods, implying a drastic change in the V2R repertoire during terrestrial adaptation. To understand the process of diversification of V2Rs in vertebrates from “fish-type” to “tetrapod-type”, we conducted an exhaustive search for V2Rs in cartilaginous fish (chimeras, sharks, and skates) and basal ray-finned fish (reedfish, sterlet, and spotted gar), and compared them with those of teleost, coelacanth, and tetrapods. Phylogenetic and synteny analyses on 1897 V2Rs revealed that basal ray-finned fish possess unexpectedly higher number of V2Rs compared with cartilaginous fish, implying that V2R gene repertoires expanded in the common ancestor of Osteichthyes. Furthermore, reedfish and sterlet possessed various V2Rs that belonged to both “fish-type” and “tetrapod-type”, suggesting that the common ancestor of Osteichthyes possess “tetrapod-type” V2Rs although they inhabited underwater environments. Thus, the unexpected diversity of V2Rs in basal ray-finned fish may provide insight into how the olfaction of osteichthyan ancestors adapt from water to land.
The impact of COVID-19 on patients with chronic pain seeking care at a tertiary pain clinic
Authors: Ziadni Maisa S. , You Dokyoung S. , Cramer Eric M. , Anderson Steven R. , Hettie Gabrielle , Darnall Beth D. , Mackey Sean C. 
Published: April 2022

AbstractEmpirical data on the health impacts of the COVID-19 pandemic remain scarce, especially among patients with chronic pain. We conducted a cross-sectional study matched by season to examine patient-reported health symptoms among patients with chronic pain pre- and post-COVID-19 pandemic onset. Survey responses were analyzed from 7535 patients during their initial visit at a tertiary pain clinic between April 2017–October 2020. Surveys included measures of pain and pain-related physical, emotional, and social function. The post-COVID-19 onset cohort included 1798 initial evaluations, and the control pre-COVID-19 cohort included 5737 initial evaluations. Patients were majority female, White/Caucasian, and middle-aged. The results indicated that pain ratings remained unchanged among patients after the pandemic onset. However, pain catastrophizing scores were elevated when COVID-19 cases peaked in July 2020. Pain interference, physical function, sleep impairment, and emotional support were improved in the post-COVID-19 cohort. Depression, anxiety, anger, and social isolation remained unchanged. Our findings provide evidence of encouraging resilience among patients seeking treatment for pain conditions in the face of the COVID-19 pandemic. However, our findings that pain catastrophizing increased when COVID-19 cases peaked in July 2020 suggests that future monitoring and consideration of the impacts of the pandemic on patients’ pain is warranted.