bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implanted hair-follicle-associated pluripotent (HAP) stem cells differentiating to keratinocytes, macrophages and endothelial cells accelerated cutaneous wound closure and suppressed scar formation in a mouse model
Authors: Obara Koya , Shirai Kyoumi , Hamada Yuko , Arakawa Nobuko , Hasegawa Ayami , Takaoka Nanao , Aki Ryoichi , Hoffman Robert M. , Amoh Yasuyuki 
Published: August 2022

Abstract
BackgroundPatients frequently experience physical, mental, and even financial distress because of acute or chronic wounds to the skin. In severe situations, the skin scars can be quite noticeable, cause persistent discomfort, restrict joint motion, or be mentally taxing. Hair-follicle-associated pluripotent (HAP) stem cells were discovered by our laboratory, in the bulge area of hair follicle; and can differentiate to neurons, glia, beating cardiomyocytes, keratinocyte and nascent vessel. In the present study, we determined if HAP stem cells can accelerate cutaneous wound healing in a mouse model.MethodsHAP stem cells which were grown from the upper part of vibrissa follicle and formed a sheet in culture were implanted to dorsal wounds in a mouse model. After HAP-stem-cell-sheet-implantation, progression of wound closure with time was evaluated. After wound closure, scar morphology, infiltration of dermal inflammatory cell such as macrophage and fibrocyte and dermal fibrosis were observed histologically. mRNA of TGF-β1, type I collagen alpha 2 (COL1A2) and type III　collagen alpha 1 (COL3A1) expression levels in the wound were measured by quantitative real-time PCR (RT-PCR) to assess dermal inflammation and fibrosis.ResultsHAP stem cells formed sheet which differentiated to keratinocytes, macrophages and endothelial cells in culture. After HAP-stem-cell-sheet-implantation to the dorsal wound in the mice model, it accelerated the wound closure, increased capillary-vessel-formation and suppressed macrophage and fibrocyte infiltration and collagen deposition in the dermis compared with non-implanted control mice. Also, mRNA of TGF-β1, COL1A2 and COL3A1 expression levels in the wound were decreased in the HAP-stem-cell-implanted mice compared with non-implantation control mice.ConclusionsImplantation of HAP stem cells differentiated to keratinocytes, macrophages and endothelial cells accelerated wound closure and suppressed scar formation in a mouse model, indicating clinical potential of scar-free wound healing.
Histologic evaluation of the healing potentiality of human treated dentin matrix scaffold as direct pulp capping material
Authors: Saeed Mahmoud , Grawish Mohammed 
Published: August 2022

Abstract
Aim: Find a reasonable substitution for current DPC materials.Methods: The hTDM properties were evaluated by assessing its alkalinity, antibacterial activity, and potentiality to induce cell migration compared to MTA. The acute inflammatory response was evaluated 3 days after iatrogenic pulp exposure by histological examination. However, both chronic inflammatory response and dentin-pulp complex regeneration were evaluated after 3 months.Results: The results of in vitro assessment revealed high alkalinity of MTA while hTDM exhibited optimum alkalinity. Moreover, the higher antibacterial activity and potentiality of inducing cell migration than MTA. The in vivo assessment ensured the ability of hTDM in optimizing the inflammatory response and dentin-pulp complex regeneration. However, MTA prolonged the inflammatory response and induced the formation of a thick calcific bridge toward the pulp tissue.Conclusion: hTDM semi-rigid scaffold is a promising novel DPC material.
Bone Regeneration by Hydroxyapatite-Gelatinnanocomposites
Authors: Mostafa Sahar I , Abdelfattah Nesma M , Ghorab Sayed M , Osman Manal F , Elwassefy Noha A 
Published: August 2022

Abstract
Aim of study:
Preparation and characterization of a series of new biocompatible injectable bone paste (IBP) nanocomposites, hydroxyapatite-gelatin (HA-Gel) and hydroxyapatite-Gelatin-alendronate (HA-Gel-Ald np).
Material and methods
IBP nanocomposites were synthesized from mixing different ratios of gelatin to aqueous solutions of both Ca(NO3)2.4H2O and (NH4)2HPO4 to obtain (HA-Gel np), while the target nanocomposites, HA-Gel-Ald np, were obtained by submitting aqueous solution of alendronate (Ald) to HA-Gel np nanocomposites. These composites crystallinity were analyzed by FTIR and XRD, and their morphology were characterized by scanning electron microscopy (SEM) and EDX measurements. XRD patterns, SEM and EDX presented changes in the crystal and surface structure from HA to HA-Gel np to HA-Gel-Ald np. Furthermore, the cytotoxicity of the nanocomposites on stem cells were assessed using MTT assay.
Results
The physico-chemical measurements, FTIR, XRD, SEM and EDX indicated the success in isolating the nanocomposites, HA-Gel np and HA-Gel-Ald np, with different ratios. Although the cytotoxicity data show significant effect of the prepared IBP nanocomposites (p = 0.00), their interaction together had no significant effect (p = 0.624).
Predictions based on different climate change scenarios: The habitat of typical locust species is shrinking in Xinjiang, China, and Kazakhstan
Authors: Wu Rui , Guan Jingyun , Wu Jianguo , Ju Xifeng , An Qinghui , Zheng Jianghua 
Published: August 2022

Abstract
Climate change, especially climate extremes, can increase the uncertainty of locust outbreaks. The Italian locust(Calliptamus italicus L.), Asian Migratory locust (Locusta migratoria migratoria L.), and Siberian locust(Gomphocerus sibiricus) are common pests widely distributed in the semidesert grasslands of Central Asia and its surrounding regions.Predicting locust geographic distribution changes and future habitats accounting for climate warming is essential to effectively prevent large and sudden locust outbreaks. The MaxEnt model was used in this study to identify environmental factors that impact the distribution of the three typical locust species and to display the probable appropriate regions and uncertainty of typical locust species habitats under different current and future climatic scenarios. Our results showed that soil surface sand content, slope, mean precipitation during the hottest season, and precipitation seasonality were the key environmental variables affecting locust distribution in the region. The three locust species are mainly distributed in the upstream region of the Irtysh River, the Alatao Mountain region, the northern slopes of the Tianshan Mountains, around Sayram Lake, the eastern part of the Alakol Lake region, the Tekes River region, the western part of Ulungur Lake, the Ili River, the upstream region of the Tarim River. According to several climate projections, the area of potential habitat for the three most common locust species will decrease by 3.9 x 104 − 4.6 x 104 km2 by the 2030s and by 6.4 x 104 − 10.6 x 104 km2 by the 2050s. Although the suitable area is shrinking, the climate is becoming more extreme, and the high suitability area is expanding, so the risk of infestation should be taken seriously. This study on locust habitat change under climate change provides a scientific basis for the scientific prevention and control of locust disasters and the sustainable development of the grassland environment in China and Kazakhstan in the context of global warming and intergovernmental cooperation measures.
Development and characterisation of 26 polymorphic microsatellite markers in striped catfish Pangasianodon hypophthalmus
Authors: Nguyen Loc Hong , Hellemans Bart , Nguyen Thong Hoang , Volckaert Filip A.M. , Nguyen Sang Van 
Published: August 2022

AbstractBackground: The striped catfish,Pangasionodon hypophthalmus, is one of the most important cultured fish species worldwide. Although genetic management and improvement based on molecular approaches have a high potential, few applications are available due to the lack of genomic resources.Methods and results: We used a draft genome of striped catfish to develop 26 polymorphic DNA microsatellite markers. Markers were validated on 62 individuals from three wild populations. The 26 loci were highly polymorphic, with 7 to 21 alleles per locus for a total of 342 alleles. Null alleles were observed at 6 of the 26 loci. Polymorphism Information Content (PIC) values pointed to a high level of polymorphism (PIC>0.5) at all tested loci. Observed and expected heterozygosity ranged from 0.532 to 0.919 and 0.584 to 0.891, respectively. The Tonlé Sap and Lower Mekong samples were similar and differed from the Kratié sample.Conclusions:The 26 microsatellite markers represent an additional and valuable source for population genetic studies, parentage assignment, the estimation of genetic parameters for broodstock management and marker assisted selection in striped catfish.
Quantitative analysis and survey of contamination 9,10-anthraquinone in Chinese Liupao tea during aging
Authors: Liang Jianfeng , Li Ya , Bin Yuejing , Qiao Ruying , Ke Lijian , Zhong Shuiqiao , liang yanni 
Published: August 2022

Abstract
Recently, 9,10-anthraquinone(AQ) contamination in Chinese Liupao tea has attracted much attention because the tea for exportation must meet the EU limit(20 µg·kg-1). In this study, the sample pretreatment methods were optimized from the aspects of extraction reagent and purification column filler. Finally, n-hexane acetone was selected as the optimal extraction agent. When the content of Florisil in the purification column was 1.0 g, the purification effect was the best. The new method reduces the limit of quantification (LOQs) of AQ to 10 μg·kg-1, and the detection accuracy has also been further improved. The recovery of 9,10-anthraquinone-fortified tea samples (20-100 μg·kg-1) was 94.51-100.44%, and the relative standard deviation (RSD) was less than 1.31%. In this study, 98 Liupao tea samples on the market were tested by the new method. It was found that 61 samples were positive (occurrence rate 63.3%), and the AQ content of 55 positive samples was higher than the EU limit (20 µg·kg-1). This study also found that the pollution of AQ in Liupao tea increased with the growth of aging time. The source of AQ in the aging process of Liupao tea will be the focus of further research.
Oblique electrostatic waves in a dusty plasma with (r; q) distributed electrons for Saturn’s magnetosphere
Authors: shumaila Shumaila , Jahangir R. , Saba Faiza 
Published: August 2022

Abstract
The linear and nonlinear propagation characteristics of dust ion acoustic waves (DIAWs) are investigated in a collisionless, magnetized dusty plasma. The system is composed of (r; q) distributed electrons with warm ions and stationary dust and propagation of the wave is taken in the x-z plane. The linear properties of the system are studied for the plasma parameters of Saturn magnetosphere are studied by the dispersion relation. The characteristics of oblique propagation of dust ion acoustic solitary waves (DIASWs) are studied by deriving Korteweg de Vries (KdV) equation and the critical point is determined at which the nature of solitons changes. The infuence of various parameters, namely, obliqueness, magnetic field, densities, temperatures, and spectral indices of the (r; q) distributed electrons on DIASWs is investigated for Saturn’s magnetosphere. The DIASWs of (r; q) distributed electrons are also compared with Maxwellian electrons. The present work might be helpful to study other astrophysical and space systems where dusty plasmas and (r; q) distribution are predicted.
Eavaluation on performance of Eco-friendly Engineered Cementitious Composite for Sustainability Management
Authors: Chowdary Muppalla Venkata Sai Surya Pratap , Asadi SS. 
Published: August 2022

Abstract
Engineered Cementitious Composite (ECC) is one of the versatile building components. The characteristics of materials and curing period are major factors that will influence the eco-friendly nature, and performance of the composite. In this experimental analysis, the impact of alkaline treated sisal fibres with different combinations of Polyvinyl alcohol (PVA) fibres (Mix-1 is 2% Sisal fibres, Mix-2 is 0.5% Sisal fibres + 1.5% PVA fibres) on the characteristics of Indian Zone II fine aggregates (50% river sand + 50% Manufactured sand) based ECC analyzed. For this, high fly ash to Cement dosage (f/c) of 1.4 is considered. These sisal fibres and f/c ratio are responsible for reduction in the disposal (or) environmental problems. The compressive strength of composite slightly improved from Mix-1 to Mix-2 at 28days with the decreased content of sisal fibres. But the split tensile and flexural characteristics highly increased by 8.33%, 5.45% from Mix-1 to Mix-2, respectively. These characteristics highly improved with the increased curing period (28days to 120days). The durability of the composite significantly influenced from 28days to 56days, but slightly improved with the decreased content of sisal fibres. To analyze these characteristics, SEM and XRD analysis are performed. Portlandite and quartz content was highly reduced (formation of C-S-H gel) from 28days to 56days due to hydration of unhydrated fly ash, fine aggregates, and other materials. The microstructure of both the mixes is densified with this gel. Mix-2 is more densified than Mix-1 due to the properties and arrangement of sisal fibres. These fibres are appropriately arranged in the partial replacement of PVA fibres compared to full dosage.
Composition-Designed High Entropy Perovskite Oxides for Oxygen Evolution Catalysis
Authors: Okazaki Yuichi , Fujita Yushi , Murata Hidenobu , Masuyama Naoki , Nojima Yusuke , Ikeno Hidekazu , Yagi Shunsuke , Yamada Ikuya 
Published: August 2022

Abstract
Oxygen evolution reaction (OER) catalysts play an essential role in energy-conversion electrochemical reactions. High entropy oxides (HEOs) were recently investigated as promising candidates to realize highly active and cost-effective OER catalysts. The vast composition space for HEOs needs considerable efforts to find possible catalysts, which disturbs the further development beyond simple chemical compositions like equimolar ones. In this study, we conducted the fast and efficient design of the perovskite HEOs of La(Cr, Mn, Fe, Co, Ni)O3 with high OER catalytic activity using Bayesian optimization and elucidated the relationship between chemical compositions and OER catalytic activities. The HEOs with optimized compositions exhibited much higher activities than equimolar LaCr1/5Mn1/5Fe1/5Co1/5Ni1/5O3, which was previously reported as an active catalyst. Bayesian optimization adjusted the concentrations of OER active elements of Fe, Co, and Ni to enhance the catalytic activities and provided the insight that inactive elements (Cr and Mn) in HEOs even promoted the OER activities. These findings suggest the solution of data-based predictions to improve catalytic performances in multi-element transition metal oxides.
Novel synthesis of metal-organic-framework-based film with enhanced mechanical properties, biodegradability and antibacterial performance for packaging applications
Authors: Ananthi P. , Hemkumar K. , Subasini S. , Pius Anitha 
Published: August 2022

Abstract
Bio-nanocomposite-based packaging materials have gained interest due to their possible applications in food packaging. Cellulose acetate (CA) is a biopolymer obtained by acetylation of cellulose and has characteristics such as biocompatibility, biodegradability and high transparency. Introducing Metal-Organic Frameworks (MOFs) offer good mechanical strength, unique surface area and both chemical and thermally stable, making them great supporting materials in the development of polymer-based packaging materials. Among them, Fe-MIL-88A an iron-based MOF, integrated with CA and spinach extract was added to the prepared material in different compositions and cast as a film. The Spinach loaded, Fe-MIL-88A integrated cellulose acetate film significantly enhanced the tensile strength, water vapour permeability, and anti-microbial activity. The prepared film is then characterized using a scanning electron microscope (SEM), Fourier transforms infrared spectrometer (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). Further, studies on mechanical properties, degradation test and real-time applications of the prepared films were carried out.
Hybrid Closed Wedge High Tibial Osteotomy Maintains the Leg Length after Surgery Compared to Open Wedge High Tibial Osteotomy
Authors: Takagawa Shu , Kobayashi Naomi , Yukizawa Yohei , Hirata Yasuhide , Honda Hideki , Inaba Yutaka 
Published: August 2022

Abstract
Background: Hybrid closed wedge high tibial osteotomy (h-CWHTO) is an improved surgical technique compared with conventional CWHTO that can help reduce the amount of bone block removed. To our best knowledge, there are no reports on changes in leg length after h-CWHTO. This study aimed to assess the change in leg length after open wedge high tibial osteotomy (OWHTO) and h-CWHTO and verify whether the change in leg length was associated with preoperative radiographic factors and the opening or closing of bone distance.Methods: We retrospectively evaluated the data of patients treated via OWHTO and h-CWHTO between 2016 and 2019. Data from 57 cases of OWHTO and 31 cases of h-CWHTO were included in the analysis. Standing full-length anteroposterior radiographs were obtained preoperatively and one year postoperatively. Changes in the lower leg and tibial length were measured using radiography, and the opening or closing of the bone distance was decided via operative planning. Flexion contracture was examined preoperatively and one year postoperatively using a goniometer, and the correlation factors and changes in leg length were analyzed using Spearman’s rank correlation.Results: In the OWHTO group, the lower leg was significantly longer by a mean of length of 6.0±8.7 mm; however, a significant difference was not observed in the h-CWHTO group (mean, -0.56±11.6 mm). Additionally, flexion contracture significantly improved from -7.1±7.0 degrees to -4.7±6.2 degrees in the h-CWHTO group alone. There were no radiographic factors or bone opening or closing distances associated with changes in leg length.Conclusion: While OWHTO extended the lower leg, h-CWHTO could maintain the leg length after surgery. The changes in leg length after OWHTO and h-CWHTO were not predictable based on the preoperative factors.Level of Evidence: Level Ⅲ Retrospective Cohort Study
Diagnostic models for fever of unknown origin based on 18F-FDG PET/CT: A prospective study in China
Authors: Chen Jia , Xing MingYou , Xu Dong , Xie NaNa , Zhang WenCong , Ruan QiuRong , Song JianXin 
Published: August 2022

Abstract
Background: This study aims to analyze the PET/CT characteristics of different causes of fever of unknown origin (FUO) and identify independent predictors to develop a suitable diagnostic model for distinguishing between these causes. A total of 524 patients with classical FUO who underwent PET/CT were prospectively studied. The diagnostic performance of PET/CT imaging was analyzed, and relevant clinical parameters that could improve diagnostic efficacy were identified. The model was established using the data of 369 patients and the other 155 patients comprised the validation cohort for verifying the diagnostic performance of the model.Results: The metabolic characteristics of the “hottest” lesion, the spleen, bone marrow, and lymph nodes, were different among the various causes. The addition of clinical parameters could improve the diagnostic efficacy of PET/CT in diagnosing FUO. In the testing cohort, the AUCs of the infection prediction model, the malignancy diagnostic model, and the noninfectious inflammatory disease (NIID) prediction model were 0.89 (0.86-0.92), 0.94 (0.92-0.97), and 0.95 (0.93-0.97), respectively. The results with the validation cohort were similar; the corresponding AUCs were 0.88 (0.82-0.93), 0.93 (0.89-0.98), and 0.95 (0.92-0.99), respectively.Conclusions: 18F-FDG PET/CT has a certain level of sensitivity and accuracy in diagnosing FUO, which can be further improved by combining it with clinical parameters. Diagnostic models based on PET/CT show excellent performance and can be used as a reliable tool to discriminate the cause of FUO.Trial registration: This study (A two-step method apparently improved the physicians’ level of diagnosis decision-making for adult patients with FUO) was registered on the website http://www.clinical-trials.gov on January 14, 2014, with registration number NCT02035670.
Construction of a Cuproptosis-Related Signature for Predicting Prognosis and Immune Response in Clear Cell Renal Cell Carcinoma
Authors: Cui Yankang , Zhang Jing , Shen Tianyi , Jiang Aiming , Xu Feng , Fang Bo , Shi Changjie , Yi Xiaoming , Lv Huichen , Wang Suchun , He Haowei , Tang Hao , Ge Jingping , Qu Le 
Published: August 2022

Abstract
Background
Cuproptosis is a new form of programmed cell death which induced by copper. The signature of cuproptosis related lncRNAs and mRNAs (CRLM) has not been reported before in clear cell renal cell carcinoma.
Methods
The RNA-seq, clinical data, and single nucleotide variants (SNV) data of clear cell renal cell carcinoma (ccRCC) were obtained from the Cancer Genome Atlas (TCGA). Cuproptosis related long non-coding RNAs (lncRNAs) were identified via Pearson’s test. Cox regression and Least Absolute Shrinkage and Selection Operator (LASSO) regression analyses were used to further screen prognosis related lncRNAs and mRNAs for signature construction. Cases were then randomly divided into training and testing set groups at ratio 1:1 to validate the cuproptosis signature. Kaplan–Meier survival analysis, receiver operating characteristic (ROC) curve analysis, nomogram, principal components analysis (PCA), gene set enrichment analyses (GSEA), mutation landscape, and therapies response were subsequently conducted with R or perl algorithms. A Japanese ccRCC cohort (n = 98) was used to validate this signature.
Results
A total of 245 lncRNAs were identified as cuproptosis related. Three of them (AL731577.2, LINC00460, AL133215.2) and cuproptosis related mRNA DBT were finally incorporated into the signature construction. High-risk group patients suffered from lower survival rate. The area under curve (AUC) of the novel signature for 1-, 3-, 5-year survival rates was 0.731, 0.718, 0.745, respectively. The concordance index (C-index) was 0.692. The Japanese cohort showed a good consistence with the results derived from the TCGA dataset. Tumor mutation burden (TMB) was also found to be a risking factor for ccRCC patients. Immune related Gene ontology terms were significantly enriched in high-risk patients. Besides, low-risk patients may be more sensitive to immune check inhibitors (ICIs), Sunitinib, and Pazopanib. High-risk patients may be more sensitive to Sorafenib.
Conclusion
The cuproptosis related signature is a promising and potential prognostic tool in predicting the survival of patients with ccRCC. It could contribute to precise and individualized ccRCC treatment.