bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-942-5p targeting the IFI27 gene regulates HCT-8 cell apoptosis via a TRAIL-dependent pathway during the early phase of Cryptosporidium parvum infection
Authors: Xie Fujie , Zhang Yajun , Li Juanfeng , Sun Lulu , Zhang Longxian , Qi Meng , Zhang Sumei , Jian Fuchun , Li Xiaoying , Li Junqiang , Ning Changsheng , Wang Rongjun 
Published: August 2022

Abstract
Background
MicroRNAs (miRNAs) are involved in the regulation of both the innate and adaptive immune response to Cryptosporidium parvum infection. We previously reported that C. parvum upregulated miR‑942‑5p expression in HCT‑8 cells via TLR2/TLR4‑NF‑κB signaling. In the present study, the role of miRNA-942-5p in the regulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated HCT-8 cell apoptosis induced by C. parvum was investigated.

Methods
Quantitative real-time polymerase chain reaction, western blotting, flow cytometry, and immunofluorescence were used for analysis.

Results
Forced expression of miRNA-942-5p resulted in decreased apoptosis and an increased C. parvum burden in HCT-8 cells. The opposite results were observed using the suppressed expression of miRNA-942-5p. The miRNA-942-5p led to the translational suppression of IFI27 gene through targeting the 3’-untranslated region of the IFI27 gene. Moreover, overexpression of the IFI27 gene produced a high apoptotic ratio and low C. parvum burden. In contrast, a low apoptotic ratio and a high C. parvum burden were observed following downregulation of the IFI27 gene. Both miR-942-5p and the IFI27 gene influenced TRAIL and caspase-8 expression induced by C. parvum in HCT-8 cells. Moreover, TRAIL promoted HCT-8 cell apoptosis in a concentration-dependent manner.

Conclusions
These data suggested that C. parvum induced the downregulation of IFI27 via relief of miR-942-5p-mediated translational suppression. IFI27 downregulation was affected the burden of C. parvum by regulating HCT-8 cell apoptosis through TRAIL-dependent pathways. Future studies should determine the mechanisms by which C. parvum infection increases miR-942-5p expression and the role of miR-942-5p in hosts' anti-C. parvum immunity in vivo.

Graphical Abstract
Serum granulocyte-macrophage colony-stimulating factor (GM-CSF) is increased in patients with active radiographic axial spondyloarthritis and persists despite anti-TNF treatment
Authors: Papagoras Charalampos , Tsiami Styliani , Chrysanthopoulou Akrivi , Mitroulis Ioannis , Baraliakos Xenofon 
Published: August 2022

Abstract
Background
Accumulating evidence supports the role of monocytes and neutrophils in radiographic axSpA (r-axSpA). Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a growth factor for both leukocyte lineages and a pro-inflammatory cytokine activating myeloid cells and promoting osteoclastogenesis. It acts through the JAK-STAT pathway. We measured serum GM-CSF and markers of bone metabolism in patients with r-axSpA before and after anti-TNF treatment.

Methods
Patients with active r-axSpA despite treatment with NSAIDs, all eligible for treatment with a biologic agent, were recruited. Healthy donors were sampled as controls. Serum was collected before (baseline) and after 4–6 months (follow-up) of anti-TNF treatment and the following molecules were measured with ELISA: GM-CSF, sclerostin (SOST), and dickkopf-1 (Dkk-1).

Results
Twelve r-axSpA patients (7 males, 5 females, median age 37 years) with a median disease duration of 1 year and 16 age- and sex-matched controls were included. At baseline, patients had mean BASDAI 6.3±2 and ASDAS 3.2±0.7, which decreased to 4.1±1.7 and 2.2±0.6 at follow-up, respectively. At baseline, r-axSpA patients had significantly higher mean serum levels of GM-CSF (150 vs 62pg/ml, p=0.049), significantly lower Dkk-1 (1228 vs 3052pg/ml, p=0.001), but similar levels of SOST (369 vs 544pg/ml, p=0.144) compared to controls. Anti-TNF treatment did not affect GM-CSF, Dkk-1, or SOST levels. Spearman correlation analysis showed that GM-CSF correlated positively with ASDAS at baseline (r=0.61, p=0.039), while no correlations were identified between bone markers (Dkk-1, SOST) on one hand and GM-CSF or disease activity indices on the other.

Conclusions
GM-CSF is increased in patients with active AS and strongly correlates with disease activity. TNF inhibition does not affect GM-SCF levels, despite improving disease activity. GM-CSF may represent an important pathway responsible for residual inflammation during TNF blockade, but also a potential target of JAK inhibitors, explaining their efficacy in r-axSpA.
A duodenal gastrointestinal stromal tumor mimicking a pancreatic neuroendocrine tumor: a case report
Authors: Inoue Masashi , Ohmori Ichiro , Watanabe Atsuhiro , Kajikawa Ryujiro , Kajiwara Ryotaro , Sawada Hiroyuki , Miyamoto Kazuaki , Ikeda Masahiro , Toyota Kazuhiro , Sadamoto Seiji , Takahashi Tadateru 
Published: August 2022

Abstract
Background
Duodenal gastrointestinal stromal tumors are rare. If tumor growth is extraluminal and involves the head of the pancreas, the diagnosis of a duodenal gastrointestinal stromal tumor is difficult.

Case presentation
A 44-year-old Japanese woman was referred to our hospital with anemia. An enhanced computed tomography scan showed a hypervascular mass 30 mm in diameter, but the origin of the tumor, either the duodenum or the head of the pancreas, was unclear. Upper gastrointestinal endoscopy revealed bulging accompanied by erosion and redness in part of the duodenal bulb. Mucosal biopsy was not diagnostic. Endoscopic ultrasound fine-needle aspiration was difficult to perform because a pulsating blood vessel was present in the region to be punctured. These findings led to a diagnosis of pancreatic neuroendocrine tumor invasion to the duodenum. The patient underwent pancreaticoduodenectomy. Histologically, the tumor was made up of spindle-shaped cells immunohistochemically positive for c-Kit and CD34. The tumor was ultimately diagnosed as a duodenal gastrointestinal stromal tumor.

Conclusion
Extraluminal duodenal gastrointestinal stromal tumors are rare and mimic pancreatic neuroendocrine tumors. Endoscopic ultrasound fine-needle aspiration is useful for preoperative diagnosis, but it is not possible in some cases. Intraoperative diagnosis based on a completely resected specimen of the tumor may be useful for modifying the surgical technique.
Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis
Authors: Xiao Yuhang , Tang Jinming , Yang Desong , Zhang Baihua , Wu Jie , Wu Zhining , Liao Qianjin , Wang Hui , Wang Wenxiang , Su Min 
Published: August 2022

Abstract
Introduction
Esophageal squamous cell carcinoma (ESCC) represents a major malignancy with poor clinical outcomes. Long noncoding RNAs (lncRNAs) are known to regulate the development and progression of multiple cancers. However, how lncRNAs are involved in ESCC is currently undefined.

Methods
LIPH-4 levels in ESCC tissue specimens and cells were assessed by qRT-PCR. The biological function of LIPH-4 was examined in cell and animal studies, applying CCK-8, EdU, colony formation and flow cytometry assays as well as xenograft model experiments. The underlying mechanisms of action of LIPH-4 were explored through bioinformatics, luciferase reporter assay, RNA-immunoprecipitation assay and immunoblot.

Results
We identified a novel lncRNA, LIPH-4, which showed elevated amounts in ESCC tissues and positive correlations with increased tumor size and poor prognosis in ESCC patients. Functional studies showed that LIPH-4 promoted the growth, mediated cell cycle progression and inhibited apoptosis in ESCC cells in vitro, and promoted tumor growth in mice. In terms of mechanism, LIPH-4 could bind to miR-216b and act as a competing endogenous RNA (ceRNA) to induce the expression of miR-216’s target gene IGF2BP2. LIPH-4 played an oncogenic role in ESCC through the miR-216b/IGF2BP2 axis.

Conclusions
This study suggested that LIPH-4 functions as a novel oncogenic lncRNA by acting as a ceRNA for miR-216b to regulate IGF2BP2, indicating LIPH-4 likely constitutes a prognostic biomarker and therapeutic target in ESCC.
Warming up for a better fever: a randomized pilot study in pediatric oncology
Authors: Krafft Hanno S. , Raak Christa K. , Jenetzky Ekkehart , Zuzak Tycho J. , Längler Alfred , Martin David D. 
Published: August 2022

AbstractBackgroundFever in children is a major problem in pediatric oncology. Usual management leads to immediate antibiotic and antipyretic therapy, although there is consensus that antipyretic therapy should not be utilized with the sole aim of reducing body temperature. Increased body temperature during fever appears to be an effective modifier in terms of viral replication and enhanced host defense mechanisms against pathogens. Therefore, it might be beneficial to support febrile patients by applying gentle heat during the onset of fever to help the body to reach its new thermoregulatory set point.MethodsA randomized pilot study over 6 months will be conducted in a pediatric oncology department in an academic hospital in Germany. This study is a preparation for a multicenter clinical trial with two parallel groups concerning the efficacy of heat application vs. treatment as usual. One of the inclusion criteria is body temperatures ≥ 38.0 °C inn= 24 cases of patients receiving chemotherapy aged 18 months to 17 years. The first intervention consists of gentle heat application with hot water bottles at any sign of illness and onset of fever. The aim is to achieve a warm periphery equilibrated to trunk temperature of less than 0.5 °C. The second intervention is the avoidance of antipyretics. The control group receives the standard antipyretic treatment from the participating hospital. The purposes of this pilot study are proof of principle of intervention, evaluation of safety, feasibility, definition of endpoints, and to receive basic data for sample size calculation and needed resources.DiscussionThe main goal is to improve the care of children with cancer by providing the best possible support for febrile episodes. If fever support by heat reduces discomfort, administration of antipyretics and maybe even antibiotics, this would be an advancement in oncological fever management. This pilot study is intended to provide a basis for a main, multicenter, randomized trial and demonstrate the practicability of heat application in febrile patients in pediatric oncology.Trial registrationGerman Clinical Trials Register (DRKS),DRKS00028273. Registered on 14 April 2022
Dissimilar donuts in the sky? Effects of a pressure singularity on the circular photon orbits and shadow of a cosmological black hole
Authors: Odintsov S. D. , Oikonomou V. K. 
Published: August 2022

Abstract
The black hole observations obtained so far indicate one thing: similar “donuts” exist in the sky. But what if some of the observed black hole shadows that will be obtained in the future are different from the others? In this work the aim is to show that a difference in the shadow of some observed black holes in the future might explain the H
0-tension problem. In this letter we investigate the possible effects of a pressure cosmological singularity on the circular photon orbits and the shadow of galactic supermassive black holes at cosmological redshifts. Since the pressure singularity is a global event in the Universe, the effects of the pressure singularity will be imposed on supermassive black holes at a specific redshift. As we show, the pressure singularity affects the circular photon orbits around cosmological black holes described by the McVittie metric, and specifically, for some time before the time instance that the singularity occurs, the photon orbits do not exist. We discuss the possible effects of the absence of circular photon orbits on the shadow of these black holes. Our idea indicates that if a pressure singularity occurred in the near past, then this could have a direct imprint on the shadow of supermassive galactic black holes at the redshift corresponding to the time instance that the singularity occurred in the past. Thus, if a sample of shadows is observed in the future for redshifts

, and for a specific redshift differences are found in the shadows, this could be an indication that a pressure singularity occurred, and this global event might resolve the H
0-tension as discussed in previous work. However, the observation of several shadows at redshifts

is a rather far future task.
Convolutional Neural Network for Predicting Thyroid Cancer Based on Ultrasound Elastography Image of Perinodular Region
Authors: Hu Lei , Pei Chong , Xie Li , Liu Zhen , He Nianan , Lv Weifu 
Published: August 2022

Abstract
We aimed to develop deep learning models based on perinodular regions’ shear-wave elastography (SWE) images and ultrasound (US) images of thyroid nodules (TNs) and determine their performances in predicting thyroid cancer. A total of 1747 American College of Radiology Thyroid Imaging Reporting & Data System 4 (TR4) thyroid nodules (TNs) in 1582 patients were included in this retrospective study. US images, SWE images, and 2 quantitative SWE parameters (maximum elasticity of TNs; 5-point average maximum elasticity of TNs) were obtained. Based on US and SWE images of TNs and perinodular tissue, respectively, 7 single-image convolutional neural networks (CNN) models [US, internal SWE, 0.5 mm SWE, 1.0 mm SWE, 1.5 mm SWE, 2.0 mm SWE of perinodular tissue, and whole SWE region of interest (ROI) image] and another 6 fusional-image CNN models (US + internal SWE, US + 0.5 mm SWE, US + 1.0 mm SWE, US + 1.5 mm SWE, US + 2.0 mm SWE, US + ROI SWE) were established using RestNet18. All of the CNN models and quantitative SWE parameters were built on a training cohort (1247 TNs) and evaluated on a validation cohort (500 TNs). In predicting thyroid cancer, US + 2.0 mm SWE image CNN model obtained the highest area under the curve in 10 mm &lt; TNs ≤ 20 mm (0.95 for training; 0.92 for validation) and TNs &gt; 20 mm (0.95 for training; 0.92 for validation), while US + 1.0 mm SWE image CNN model obtained the highest area under the curve in TNs ≤ 10 mm (0.95 for training; 0.92 for validation). The CNN models based on the fusion of SWE segmentation images and US images improve the radiological diagnostic accuracy of thyroid cancer.
Continuous Microdebrider-Based Dynamic Subcortical Motor Mapping: A Technical Advance in Tubular Retractor–Assisted Surgery
Authors: Gallagher Mathew J. , Lavrador Jose P. , Coelho Pedro , Mirallave-Pescador Ana , Bleil Cristina , Gullan Richard , Ashkan Keyoumars , Vergani Francesco , Bhangoo Ranjeev 
Published: August 2022

BACKGROUND:
Transsulcal minimally invasive parafasicular (TsMIP) approaches to brain tumor resection use tubular retractors to minimize iatrogenic brain injury. Dynamic cortical and subcortical continuous neurophysiological mapping facilitates safer resection of motor-eloquent tumors.

OBJECTIVE:
To describe a new technique to address the challenge of combining TsMIP with tubular retractors and dynamic subcortical mapping using a single electrified stimulating microdebrider instrument.

METHODS:
We adapted the NICO Myriad microdebrider with continuous monopolar stimulation electrification using high-frequency stimulation with the train-of-5 technique. We performed continuous subcortical mapping using this device and compared it with standard dynamic monopolar subcortical mapping using a suction stimulation device. We found no significant difference in recorded stimulation response.

RESULTS:
Using a single operating instrument that provides synchronous tumor resection and monopolar subcortical mapping with the NICO Brainpath tubular retractor, we observed increased degrees of movement, faster surgical resection times with an enlarged working channel down the retractor, and improved safety because the stimulating probe sits 2 mm deep to the resection window.

CONCLUSION:
We show that the adapted device is reliable and provides similar stimulation response as conventional subcortical mapping. We advocate the use of our adapted microdebrider in TsMIP tubular retractor approaches.
Stereotactic-Guided Transcerebellar Cisternoperitoneal Shunt Placement for Idiopathic Intracranial Hypertension
Authors: Cho Steve S. , Wakim Andre A. , Teng Clare W. , Sarris Christina E. , Smith Kris A. 
Published: August 2022

BACKGROUND:
Idiopathic intracranial hypertension (IIH) can cause debilitating symptoms and optic nerve ischemia if untreated. Cerebrospinal fluid diversion is often necessary to reduce intracranial pressure; however, current ventriculoperitoneal and lumboperitoneal shunting techniques have high failure rates in patients with IIH.

OBJECTIVE:
To describe our experience treating IIH with a novel stereotactic-guided transcerebellar cisternoperitoneal shunt (SGTC-CPS) technique that places the proximal shunt catheter in the posterior cisterna magnum.

METHODS:
Retrospective perioperative and postoperative data from all patients who underwent SGTC-CPS placement for IIH from March 1, 2015, to December 31, 2020, were analyzed. Patients were positioned as for ventriculoperitoneal shunt placement but with the head turned farther laterally to adequately expose the retrosigmoid space. Using neuronavigation, an opening was made near the transverse-sigmoid junction, and the proximal catheter was inserted transcerebellarly into the posterior foramen magnum.

RESULTS:
Thirty-two patients underwent SGTC-CPS placement (29 female; mean body mass index, 36.0 ± 7.5; 14 with prior shunt failures). The mean procedure time for shunt placement was 145 minutes. No intraoperative complications occurred, and all patients were discharged uneventfully. At the 6-month follow-up, 81% of patients (21 of 26) had relief of their presenting symptoms. Shunt survival without revision was 86% (25 of 29) at 1 year and 67% (10 of 15) at 3 years, with no infections.

CONCLUSION:
The SGTC-CPS offers an alternative solution for cerebrospinal fluid diversion in patients with IIH and demonstrates a lower failure rate and more durable symptom relief compared with ventriculoperitoneal or lumboperitoneal shunt placement. Using proper techniques and equipment promotes safe and facile placement of the proximal catheter.
Complications Associated With Ventriculoperitoneal Shunt Surgery for Normal Pressure Hydrocephalus Using Stereotactic Navigation and Abdominal Laparoscopy: A Single-Institution Case Series
Authors: Golubovsky Joshua L. , Liao James , Hogue Olivia , Pucci Francesco , Rammo Richard , Lipman Jeremy , Aminian Ali , Nagel Sean J. 
Published: August 2022

BACKGROUND:
Normal pressure hydrocephalus (NPH) is characterized by cerebral ventriculomegaly and the triad of magnetic gait, urinary incontinence, and cognitive impairment. Treatment includes ventriculoperitoneal (VP) shunt surgery.

OBJECTIVE:
To evaluate complication rates in a cohort of patients undergoing VP shunt surgery with stereotactic proximal catheter navigation and laparoscopic distal catheter placement.

METHODS:
This study was a retrospective consecutive cohort analysis of 117 patients with NPH undergoing VP shunt placement using both stereotactic navigation and laparoscopy from 2015 to 2020. Patients with obstructive hydrocephalus and those with central nervous system infection, intraventricular hemorrhage, Ommaya reservoirs, or undergoing shunt revision at initial encounter were excluded. Variables included demographics and comorbidities, NPH symptoms, operative details, radiographic outcomes, and rates of complications, readmissions, and reoperations within 1, 3, and 12 months. Impact of demographics and comorbidities on complication rates was assessed using Fisher exact tests.

RESULTS:
Zero patients required reoperation within 30 days. One intracranial hemorrhage was detected on immediate postoperative head computed tomography. Four patients ultimately required revision: 2 for catheter repositioning to alleviate abdominal pain, 1 ligation for a colectomy, and 1 removal for shunt infection. Patients with cardiac or other neurological comorbidities had higher rates of readmission and complications. Systemic complications totaled 12% in the first 30 days.

CONCLUSION:
The combination of intraoperative stereotactic navigation and laparoscopic assistance leads to low rates of serious complications and reoperations for VP shunt implantation in patients with NPH. These changes to surgical technique are easy to implement and may reduce the risk for this common operation.
Robot-Assisted Deep Brain Stimulation: High Accuracy and Streamlined Workflow
Authors: Giridharan Nisha , Katlowitz Kalman A. , Anand Adrish , Gadot Ron , Najera Ricardo A. , Shofty Ben , Snyder Rita , Larrinaga Christopher , Prablek Marc , Karas Patrick J. , Viswanathan Ashwin , Sheth Sameer A. 
Published: August 2022

BACKGROUND:
A number of stereotactic platforms are available for performing deep brain stimulation (DBS) lead implantation. Robot-assisted stereotaxy has emerged more recently demonstrating comparable accuracy and shorter operating room times compared with conventional frame-based systems.

OBJECTIVE:
To compare the accuracy of our streamlined robotic DBS workflow with data in the literature from frame-based and frameless systems.

METHODS:
We retrospectively reviewed 126 consecutive DBS lead placement procedures using a robotic stereotactic platform. Indications included Parkinson disease (n = 94), essential tremor (n = 21), obsessive compulsive disorder (n = 7), and dystonia (n = 4). Procedures were performed using a stereotactic frame for fixation and the frame pins as skull fiducials for robot registration. We used intraoperative fluoroscopic computed tomography for registration and postplacement verification.

RESULTS:
The mean radial error for the target point was 1.06 mm (SD: 0.55 mm, range 0.04-2.80 mm) on intraoperative fluoroscopic computed tomography. The mean operative time for an asleep, bilateral implant without implantable pulse generator placement was 238 minutes (SD: 52 minutes), and skin-to-skin procedure time was 116 minutes (SD: 42 minutes).

CONCLUSION:
We describe a streamlined workflow for DBS lead placement using robot-assisted stereotaxy with a comparable accuracy profile. Obviating the need for checking and switching coordinates, as is standard for frame-based DBS, also reduces the chance for human error and facilitates training.
Practical Use of Augmented Reality Modeling to Guide Revision Spine Surgery: An Illustrative Case of Hardware Failure and Overriding Spondyloptosis
Authors: Mozaffari Khashayar , Foster Chase H. , Rosner Michael K. 
Published: August 2022

BACKGROUND AND IMPORTANCE:
Augmented reality (AR) is a novel technology with broadening applications to neurosurgery. In deformity spine surgery, it has been primarily directed to the more precise placement of pedicle screws. However, AR may also be used to generate high fidelity three-dimensional (3D) spine models for cases of advanced deformity with existing instrumentation. We present a case in which an AR-generated 3D model was used to facilitate and expedite the removal of embedded instrumentation and guide the reduction of an overriding spondyloptotic deformity.

CLINICAL PRESENTATION:
A young adult with a remote history of a motor vehicle accident treated with long-segment posterior spinal stabilization presented with increasing back pain and difficulty sitting upright in a wheelchair. Imaging revealed pseudoarthrosis with multiple rod fractures resulting in an overriding spondyloptosis of T6 on T9. An AR-generated 3D model was useful in the intraoperative localization of rod breaks and other extensively embedded instrumentation. Real-time model thresholding expedited the safe explanation of the defunct system and correction of the spondyloptosis deformity.

CONCLUSION:
An AR-generated 3D model proved instrumental in a revision case of hardware failure and high-grade spinal deformity.