bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood
Authors: Abafogi Abdurhaman Teyib , Wu Tepeng , Lee Daekyu , Lee Jinyeop , Cho Gyoujin , Lee Luke P. , Park Sungsu 
Published: March 2022

Abstract
Sepsis is caused mainly by infection in the blood with a broad range of bacterial species. It can be diagnosed by molecular diagnostics once compounds in the blood that interfere with molecular diagnostics are removed. However, this removal relies on ultracentrifugation. Immunomagnetic separation (IMS), which typically uses antibody-conjugated silica-coated magnetic nanoparticles (Ab-SiO2-MNPs), has been widely applied to isolate specific pathogens in various types of samples, such as food and environmental samples. However, its direct use in blood samples containing bacteria is limited due to the aggregation of SiO2-MNPs in the blood and inability to isolate multiple species of bacteria causing sepsis. Results: In this study, we report the synthesis of vancomycin-conjugated polydopamine-coated (van-PDA-MNPs) enabling preconcentration of multiple bacterial species from blood without aggregation. The presence of PDA and van on MNPs was verified using transmission electron microscopy, X-ray photoelectron spectroscopy and energy disruptive spectroscopy. Unlike van-SiO2-MNPs, van-PDA-MNPs did not aggregate in the blood. Van-PDA-MNPs were able to preconcentrate several species of Gram-positive bacteria in the blood, lowering the limit of detection (LOD) to 10 colony forming units/mL by polymerase chain reaction (PCR) and quantitative PCR. This is 10 times more sensitive than the LOD obtained by PCR and qPCR with the use of van-SiO2-MNPs. Conclusion: These results suggest that PDA-MNPs do not aggregate in blood and can be conjugated with receptors, thereby improving the sensitivity of molecular diagnostics for bacteria in blood samples.
An Immune-Related Clinical-Gene-Risk-Score (CGRS) Prognosis Model for Bladder Cancer
Authors: Liu Sanhe , Duan Liqun , Wang Yiqi , Cui Diansheng , Deng Kangli , Wei Shaozhong , Dong Zhiqiang 
Published: March 2022

Abstract
Immunotherapy for bladder cancer is prevalent, while the efficacy is uncertain. To predict the outcomes of immunotherapy in bladder cancer patients, we had delineated the landscape of the tumor immune microenvironment based on two independent public databases, classified the bladder cancers in the training cohort as well as the validating cohort into the immune high and immune low subtypes, and identified 23 differentially expressed immune-related genes between the two subtype patients to build a prognostic gene risk score model. We further combined the model with other clinicopathologic signatures to develop a Clinical-Gene-Risk-Score (CGRS) prognosis model and validations of the two models in independent cohorts revealed good accuracy. Our model could provide prognostic indicators and potential immunotherapeutic targets for bladder cancers.
Microbiome analysis of bile samples in patients with choledocholithiasis and hepatobiliary disorders
Authors: Azimirad Masoumeh , Sadeghi Amir , Hosseinkhan Nazanin , Mirbagheri Seyedeh Zohre , Alebouyeh Masoud 
Published: March 2022

Abstract
Involvement of bacteria in the pathogenesis of biliary tract is largely unknown. In this study, we investigated differences in the microbiota of biliary tissue among adult patients with choledocholithiasis during endoscopic retrograde cholangiography (ERCP). 16S rDNA sequencing of bile samples, culture, and data of the medication history, underlying diseases and liver function tests were used for interpretation of differences observed in composition of bacterial taxa detected in the patients. The most common phyla found among the bile samples were included Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Cyanobacteria/Chloroplast, Fusobacteria, Acidobacteria, and Deinococcus-Thermus. Infection with anaerobic and microaerophilic bacteria showed host specificity, where Fusobacter, Prevotella, Veillonella, Propionibacter, Gemella, and Helicobacter coexist in same patients. Coexistence of anaerobic bacteria was mainly limited to patients with pancreatic cancer. Clostridium and Peptoclostridium spp. were detected in 80% and 86% of the patients, where highest relative abundance rates were detected in patients with elevated ALP levels and leukocytosis, respectively. Patients with leukocytosis presented higher rates of colonization with Pseudomonas, Cronobacter, Enterococcus, Klebsiella, Peptoclostridium, and Streptocossus. H. pylori was detected in 4 patients with and 1 patient without CBD stone. Higher diversity in the bacterial population was detected in patients with CBD stone compared with those lacking the stone, as richness of an unclassified member of Alphaproteobacteria, plus Helicobacter, Enterobacter/Cronobacter spp., Sphingomonas, Prevotella, Fusobacterium and Aeromonas were detected. Further studies are needed to show impact of these bacteria in human health or diseases through their interaction with host and virulence potential.
Integrative investigation of root-related mRNAs, lncRNAs and circRNAs of ‘Muscat Hamburg’ (Vitis vinifera L.) grapevine in response to root restriction through transcriptomic analyses
Authors: Liu Jingjing , Li Hui , Zhang Lipeng , Song Yue , He Juan , Xu Wenping , Ma Chao , Ren Yi , Liu Huaifeng 
Published: March 2022

Abstract
Background
Root restriction is a physical and ecological cultivation mode which restricts plant roots into a limited container to regulate vegetative and reproduction growth by reshaping root architecture. However, little is known about related molecular mechanisms. To uncover the root-related regulatory network of endogenous RNAs under root restriction, transcriptome-wide analyses of mRNAs, long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) involved in root development was performed.
Results
In this study, a total of 26588 mRNAs, 1971 lncRNAs and 2615 circRNAs were identified in root of annual ‘Muscat Hamburg’ grapevine by the next generation sequencing, respectively. The expression profile of mRNAs, lncRNAs and circRNA were further confirmed by the quantitative real-time PCR (qRT-PCR). Gene ontology enrichment analysis showed that a majority of the differentially expressed mRNAs, lncRNAs and circRNAs were annotated to the categories of cellular process, metabolic process, cell part, binding and catalytic activity. In addition, the regulatory network of endogenous RNAs was then constructed by the prediction of lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA, implying that these RNAs play significant regulatory roles for root architecture shaping in response to root restriction.
Conclusion
Our results, for the first time, integrated the regulatory network of competitive endogenous RNAs (ceRNAs) functions of lncRNA and circRNA, and provided a basis for studying the potential functions of non-coding RNAs during root development of grapevine.
The Therapeutic Process – Client's Perception Of Success Promoting Factors In An And Arfid – Qualitative Study
Authors: Neiva Susana Maria Fernandes da Silva Albuquerque 
Published: March 2022

Abstract
This study aimed to analyze the perception that individuals have of the success promoting factors on their own therapeutic process. Intended to understand which aspects influenced their perception and which factors were considered relevant to the decision to seek help, not to abandon treatment and their motivation to change. The study involved patients with Anorexia Nervosa (AN) and Avoiding Food Intake Disorder (ARFID), with intervention within the scope of different therapeutic models.Background: In Portugal, anorexia nervosa is one of the eating disorders with a significant increase in recent years, having been responsible for 4,485 hospitalizations between 2000 and 2014, in Portuguese public health services. Portugal has scarce research in eating disorders although they are a serious mental health problem both in this country as in Europe. Methods: The study included a sample of 9 participants who responded to the request for participation posted on the different electronic platforms. The data recollection occurred thought a semi-structured interview and the information was subjected to thematic analysisResults: This study included a total of 9 participants, 5 with a diagnose of AN and 4 with a diagnose of ARFID. All the participants were women with ages between 19 and 52 years of age (an average of 38.4 years). Similarities were found in both group for and differences.Discussion: The desire to improve/not cause suffering to family members and the availability of social/emotional support were considered relevant aspects inherent to the client. 100% of the participants considered that certain professional and personal qualities of the professional/therapist was the most relevant aspect to promote the success of the therapeutic intervention. The sample size of this study is small and therefore does not allow generalizations.Conclusions: The main objective of this study was to obtain knowledge that would allow professionals to reflect on their practice and adapt the intervention to the needs and concerns of the clients. Despite the limitations of the study, our participants provided detailed and relevant information, so we consider the results obtained to be pertinent for the proposed objective. Further research in these areas is needed to deepen the knowledge.
Sex Differences in the Prevalence and Clinical Correlates of Suicidal Ideation in Adolescent Patients with Depression in a Large Sample of Chinese
Authors: Wu Jinbo , Wang Xiaohong , Zheng Yue , Shi Jingjing , Liu Jiacheng , Yang Liying , Kang Chuanyi , Yang Wanqiu , Guan Tiefeng , Liu Sitong , Zhao Na 
Published: March 2022

Abstract
Background
Sex differences in depression have been well recognized. However, sex differences in suicidal ideation (SI) in adolescents have rarely been systematically studied. This large-scale sample explored the sex differences in the prevalence and related risk factors of SI in adolescent patients with depression.
Methods
1635 patients with depression were recruited. Demographic and clinical characteristics were collected. Suicidal ideation was assessed by an interview. Children's Depression Inventory (CDI) and Adolescent Self-Rating Life Events Check List (ASLEC) were used to evaluate depression and stressful life events.
Results
The prevalence of suicidal ideation in female patients (383/887, 43.18%) was significant higher than in male patients (242/748, 32.35%). There were sex differences in CDI, ASLEC, and all subscales of ASLEC in patients with and without SI. Correlation analysis showed that family depression history and less sleeping time were significantly associated with suicidal ideation in female patients, while the severity of depression and stressful life events were associated in both genders. Further logistic regression analysis indicated that the severity of depression and relationship problems were independent risk factors of suicidal ideation in both sexes; family depression and inadequate sleeping time were significantly associated with suicidal ideation in female patients.
Conclusions
Our findings show that there are significant sex differences in the risk and clinical correlations of suicidal ideation in adolescent depression patients. Suicidal ideation is more frequent in female patients and is associated with multiple factors in both genders.
Beta-naphthoflavone Regulates Inflammation and Coagulation of Sepsis by Raising Nrf2/Ho-1 and Involving AKT/NF-κB Signaling Pathways
Authors: Chen Wenjing , Zhang Xianwei , Ye Fanrong , Sun Yuanyuan , Zhou Hongmin , Lu Yilun , Fang Zhendong , Pan Lingling , Jiang Hao , Qian Songzan , Huang Yueyue , Yu Xueshu , Chen Zhiqiang , Pan Jingye 
Published: March 2022

Abstract
BACKGROUND During sepsis, macrophages are activated by various irritants, such as Gram-negative bacteria LPS, which leads to the transcription of inflammatory and coagulation factors, including iNOS,COX-2,IL-1β,TNF-α and TF,PAI-1.Although inflammatory responses play a key role in the host's defense against infection, overactivation of innate immune cells can lead to severe sepsis. In the course of sepsis development, a variety of factors induce inflammatory response while activating coagulation function. Activation of the coagulation system is considered to be a host immune response against infection, however, overactivation of coagulation can cause harm to the host body, resulting in sepsis induced coagulopathy (SIC).METHODSIn this study, we aimed to evaluate the anti-inflammatory and anticoagulatory effects of BNF on sepsis by constructing in vivo (cecal ligation and puncture (CLP)-induced sepsis) and in vitro (LPS-induced mouse macrophage RAW264.7) sepsis models; the mechanisms behind these effects were also studied. Various aspects of sepsis, including inflammation, coagulation, and lung function, were monitored in the sepsis models.RESULTS Results indicated that the administration of BNF in sepsis mice caused reduced expression of pro-inflammatory factors, inhibited activation of coagulation, and lesser lung damage. The levels of LPS-induced macrophages, which lead to inflammation and coagulation, were also reduced. These effects are caused by participation in the Akt/NF-κB/Nrf2/HO-1 signaling pathway.CONCLUSIONThe results demonstrated that BNF treatment reduced the inflammatory response and coagulation dysfunction caused by sepsis in in vivo and in vitro models.Thus, BNF can be adopted as a novel therapeutic option to reduce sepsis mortality;However, further clinical studies are needed to confirm these effects on human sepsis.
Quantitative Measurement of Inferior Patellar Mobility: Sex-based Differences and Relationship With the Restricted Knee Flexion Angle Due to Aging
Authors: Ohko Hiroshi , Ota Susumu 
Published: March 2022

Abstract
Objective: To develop quantitative inferior patellar mobility (IPM) measurement methods and clarify the relationship between IPM and the knee flexion angle in community-dwelling elderly women.Design: The study was performed from May 2015 to December 2017. The reference value and sex difference of IPM were evaluated in 205 healthy young adults (age: 19-21 years). Additionally, 128 healthy elderly women (age: 65-79 years) were recruited from the community to evaluate the relationship between IPM and knee flexion angle. IPMs was also compared between healthy elderly women and healthy young women. IPMs were measured objectively by using our specially designed patellofemoral arthrometer (PFA); patellar mobility was calculated by normalizing to body height. IPM reliability assessment was performed prior to all measurements. Results: The intraclass correlation coefficients for intratester and intertester reliabilities varied from 0.87 to 0.99. The normal range, based on 2 standard deviations, of inferior patellar displacement/body height was 5.9%–13.5% (young men), 5.1%–14.3% (young women), and 1.2%–8.8% (elderly women). The IPM was significantly lower in elderly women than in young women (P < 0.001). There was a significant positive correlation (r = 0.72, P < 0.01) between IPM and the knee flexion angle in healthy elderly women who were unable to fully flex the knee joint.Conclusions: Our PFA has good intratester and intertester reliability. The results suggest that IPM decreases with aging in women. IPM and the knee flexion angle are correlated among elderly women who are unable to fully flex the knee joint.
Treatment of steroid-dependent rectal sparing ulcerative colitis associated with severe autoimmune hemolytic anemia with oral mycophenolate mofetil--a case report and review of literature
Authors: Liu Xin , Sha Sumei , Xu Bin , Shi Haitao , Quan Xiaojing , Chen Fenrong , Jiang Jiong 
Published: March 2022

Abstract
Background: Patients with ulcerative colitis (UC) may experience various type of anemia, in which the most common reasons are dystrophic anemia and hemorrhagic anemia. Autoimmune hemolytic anemia (AIHA) associated with UC is seldom seen, especially Coombs-negative AIHA. Case presentation: Here we report a case of refractory anemia that cannot be explained by UC disease activity in a young male. The patient was infected with cytomegalovirus (CMV). Colonoscopy showed diffuse polypoid hyperplasia with the absence of rectal involvement. Although the UC was in remission from steroid or total enteral nutrition, but the anemia is getting worse. The Coombs test showed negative, who is subsequently diagnosed by warm autoantibodies of IgA type and treated with mycophenolate mofetil (MMF) 2g daily. Both UC and AIHA were improved from treatment.Conclusion: AIHA can be secondary to UC, infection, or drugs, in which IgA autoantibody AIHA is a special situation. When gastroenterologist and Immunologist, and hematologist encounter patients with "negative" Coombs test acquired hemolytic anemia, AIHA should be considered in the differential diagnosis. MMF may be an option for the treatment of UC patients with AIHA.
Enhanced Opto-Electronic Properties Of X-Doped (X=Al, Ga, And In) Cuo Thin Films For Photodetector Applications
Authors: Gnanasekar T. , Valanarasu S. , Das Himadri Tanaya , Chidhambaram N. , Isaac R.S. Rimal , Al-Enizi Abdullah M. , Ubaidullah Mohd , Reddy Vasudeva Reddy Minnam 
Published: March 2022

Abstract
Recent trends in optoelectronics still need a high efficient photodetector based on p-type metal oxide semiconductors. This work stands with the improvement in the performance of CuO thin films via doping with different metals into the thin films. The CuO thin films were successfully doped with 1 wt% of X (X=Al, Ga, and In) by spray pyrolysis technology. The prepared doped CuO thin films were characterized to interpret the structural, morphological, and elemental characteristics using advanced techniques. These doped CuO thin films were subjected to study the photodetection ability by analyzing optoelectronic properties. The doping also has tuned the optical and electrical properties. Among the fabricated photodetectors, the Al-doped CuO detector shows a maximum photocurrent. The CuO:Al(1.0%) thin film exhibits a high photocurrent of 2.59⨯10-6A, the responsivity of 2.82⨯10-1 AW-1, the external quantum efficiency of 66%, and detectivity of 1.45⨯1010 Jones. Compared to the other thin films, Al doping has remarkably reduced the bandgap and shows a good photosensing activity that may be due to an increase in charge carriers. These outcomes provide a way to assemble good photodetectors and tune their properties in a wide range.
Urban comprehensive carrying capacity and urbanization in Northeast China with urban shrinkage
Authors: Ren Wanxia , Xue Bing , Xie Xiao , Zhao Bingyu , Li Jingzhong , Han Bin 
Published: March 2022

Abstract
The scientific evaluation and identification of the relationship between urban comprehensive carrying capacity (UCC) and urbanization in Northeast China under serious urban shrinkage is an important basis for realizing the all-around revitalization of the region. Using a panel data set of 34 prefecture-level cities with six nonshrinking cities and 28 shrinking cities in Northeast China from 2003 to 2019, this study constructs an ordinary panel data model and a static and dynamic spatial econometric model, the spatial Durbin model (SDM), to identify the relationship between the UCC and urbanization and to empirically investigate the impacts of the key control variables on UCC improvement. The results show a significant U-shaped curve relationship between the UCC and urbanization for the shrinking cities and a significant inverted U-shaped curve relationship for the nonshrinking cities, and the spillover effect of the urbanization is not significant. Moreover, social urbanization has a significant negative spatial effect on the local and neighborhood UCC of the nonshrinking cities, while demographic and spatial urbanization have significant positive spatial effects on the local and neighborhood UCC of the shrinking cities. In addition, fixed asset investment significantly promotes the UCC of the nonshrinking and shrinking cities and has significant positive spillover effects on the neighboring regions. R&D investment has a significant positive spatial effect on only the local and neighborhood UCC of the shrinking cities, while it has a significant positive effect on only the local UCC of the nonshrinking cities. New-type urbanization, industrial structure adjustment, new infrastructure construction and technological innovation are effective measures for improving and enhancing the comprehensive carrying capacity of cities in Northeast China.
Research on Collapse Ultimate Load of Fabricated RCS Composite Frame Structure
Authors: Liu Youquan , Xiong Jingang , Wen Jiancong , Xiong Moqiang 
Published: March 2022

Abstract
In this paper for study the collapse ultimate load of the prefabricated reinforced concrete column—steel beam composite frame structure, the “new RCS beam-to-column joint” is used as the beam–to-column connection to the experimental model, the half-scale fabricated RCS space frame structure (2-story, 1×2 bay) is subjected to twice instantaneous failure experiments on the bottom of side column under different load levels. The 2A column is quickly pulled out by the traction force of the vehicle. The experimental results demonstrated that the method of dismantling the failure column can relatively truly response the progressive collapse condition. The remaining RCS structure is all in the elastic stage under twice different load level tests. The displacement time history curve has no vibration phenomenon in the first experimental. The SAP2000 finite element program is used to verify that the test results are basically the same as the numerical simulation results, and it is further explored that the collapse ultimate load value is 10.25 times of the structure design load value.
Determining the Recurrence of Diabetic Patients Recovery Time in Benishangul-Gumuz Hospitals, Ethiopia
Authors: Endalkachew  , Zelalem  
Published: March 2022

Abstract
Diabetes is a chronic, metabolic disease characterized by elevated levels of blood glucose (or blood sugar). About 90% of people with diabetes around the world have type II diabetes. It is the main global health issues and burden as well with serious health complications including heart disease, blindness, kidney failure, and lower-extremity amputations. In middle- and low-income countries the prevalence of diabetes has been rising more rapidly and needs great attention. The data from such case has been considered as recurrent event data and correlation between events should be taken into account in the analysis. Thus, the aim of the study was to identify risk factors for recurrence of diabetic patients recovery time in Benishangul-Gumuz hospitals (Assosa, Pawi and Wonbera hospital), Ethiopia. A retrospective study has been applied to obtain data on the recurrence of diabetic patient’s recovery time in Benishangul-Gumuz hospitals, Ethiopia, from September 1, 2019 to September 1, 2021. Kaplan-Meier plot (s) and Log-rank test were used for comparison of patients recovery time from diabetes; Standard Cox-PH and Shared Frailty model were used to identify factors signiﬁcantly affect the recovery time of diabetic patients, using R version 4.1.1 for data analysis. Of the total diabetic patients in this study 61.87 % experienced recurrence of diabetic. The estimated median recovery time of diabetic patient’s was 6 months. The Shared Log-normal Frailty model was chosen to be best fit for this data set, based on Likelihood Cross-Validation value. Family history, other medical history and Systolic Blood Pressure of patients were significantly affected the recovery time of diabetic patient’s.