bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue-wide Genetic and Cellular Landscape Shapes the Execution of Sequential PRC2 Functions in Neural Stem Cell Lineage Progression
Authors: Amberg Nicole , Pauler Florian M. , Streicher Carmen , Hippenmeyer Simon 
Published: April 2022

ABSTRACTThe generation of a correctly-sized cerebral cortex with all-embracing neuronal and glial cell-type diversity critically depends on faithful radial glial progenitor (RGP) cell proliferation/differentiation programs. Temporal RGP lineage progression is regulated by Polycomb Repressive Complex 2 (PRC2) and loss of PRC2 activity results in severe neurogenesis defects and microcephaly. How PRC2-dependent gene expression instructs RGP lineage progression is unknown. Here we utilize Mosaic Analysis with Double Markers (MADM)-based single cell technology and demonstrate that PRC2 is not cell-autonomously required in neurogenic RGPs but rather acts at the global tissue-wide level. Conversely, cortical astrocyte production and maturation is cell-autonomously controlled by PRC2-dependent transcriptional regulation. We thus reveal highly distinct and sequential PRC2 functions in RGP lineage progression that are dependent on complex interplays between intrinsic and tissue-wide properties. In a broader context our results imply a critical role for the genetic and cellular niche environment in neural stem cell behavior.
Screening candidate genes related to psoas muscle traits in Debao and Landrace pigs based on transcriptome analysis
Authors: Chen Chang-yi , Zeng Su-xian , Ma Yuan-ding , Zheng Jun-wen , Li Xin , Xiong Chen-yong , Zhou Hong-jin , Wei Chun-tao , Li Zong-qiang 
Published: April 2022

ABSTRACTTo identify the important genes that affect the phenotypic differences between the Landrace and Debao pigs, especially the differences in metabolism and muscle growth. Differentially expressed genes of psoas major were detected by mRNA transcriptome sequencing in Landrace and Debao pigs. By extracting the total RNA of the psoas major muscle of the Landrace pig and the Debao pig, purifying the mRNA, constructing the cDNA library, conducting transcriptome sequencing, and then through the sequencing quality evaluation, we know that the sequencing quality of this study is relatively high. A total of 17,943 genes were detected in all samples, including 17,870 known genes and 73 new genes. Defined genes with |log2FC| greater than 2 and Q-value less than 0.001, and screened them as significantly differentially expressed genes. A total of 1661 differentially expressed genes were screened from the samples of Landrace pigs and Debao pigs, among which 1255 genes were differentially up-regulated and 406 genes were differentially down-regulated. Through differential gene analysis, it is concluded that these genes are mainly involved in metabolic regulation, muscle and fat development and other processes, especially some important functional genes such as MAPK14, FOS, SIRT1, KRAS, EGR1, CDNNB1, etc. To sum up, this study used transcriptome sequencing method, and then selected differentially expressed genes between Landrace pigs and Debao pigs through data analysis, and finally screened out important genes affecting phenotypic differences, which provided genetic support for breeding better breeds in the future.
Melanoma cells release DEL-1 via small extracellular vesicles
Authors: Schnödl Marianne , Tuchmann Felix , Wenzina Judith , Neumüller Karin , Mitulović Goran , Gröger Marion , Heller Gerwin , Petzelbauer Peter 
Published: April 2022

AbstractDEL-1 (developmental endothelial locus-1) induces integrin signaling and recognizes phosphatidylserine exposed on apoptotic cells. We show that DEL-1, which is thought to be a secreted molecule, is not found in melanoma cell culture supernatants but is exported by an endosomal pathway and released via small extracellular vesicles (sEV). Proteomics of DEL-1 positive sEV, but not of DEL-1 negative sEV contain proteins associated with poor survival in cancer. To determine whether DEL-1 is suitable to predict treatment responses, we isolated sEV from plasma of melanoma patients before and 90 days of treatment with checkpoint inhibitors. Although we could not detect DEL-1 in plasma sEV even in patients with progressive disease (most likely due to the very low protein yield from sEV isolated from 1ml plasma), the principal component analysis allowed a clear differentiation between controls and patients as well as between patients before and after treatment. Interestingly, in one patient with complete regression, in the post treatment sample, the protein expression profile remained in the pre-treatment cluster. The low protein yield of patient sEV and the low patient number are clear limitations of this study, but results demonstrate that this method could have the potential to predict treatment responses.
Electron tomography analysis of the prolamellar body and its transformation into grana thylakoids in the cryofixedArabidopsiscotyledon
Authors: Liang Zizhen , Yeung Wai-Tsun , Ma Juncai , Mai Keith Ka Ki , Liu Zhongyuan , Chong Yau-Lun Felix , Cai Xiaohao , Kang Byung-Ho 
Published: April 2022

AbstractThe para-crystalline structure of prolamellar bodies (PLBs) and light-induced etioplasts-to-chloroplasts transformation have been investigated with electron microscopy methods. However, these studies suffer from chemical fixation artifacts and limited volumes of three-dimensional reconstruction. We have examined Arabidopsis thaliana cotyledon cells with electron tomography (ET) to visualize etioplasts and their conversion into chloroplasts. We employed the scanning mode of ET for imaging large volumes and high-pressure freezing to improve sample preservation. PLB tubules were arranged in a zinc blende-type lattice like carbon atoms in diamonds. Within 2 hours after illumination, the lattice collapsed from the PLB exterior and the disorganized tubules merged to form thylakoid sheets, a.k.a. pre-granal thylakoids. These pre-granal thylakoids in PLB’s vicinity folded and overlapped with each other to create grana stacks. Since the nascent pre-granal thylakoids had curved membranes in their tips, we examined the expression and localization of CURT1 proteins.CURT1Atranscript was most abundant in de-etiolating cotyledon samples, and CURT1A concentrated to the PLB periphery. Incurt1aetioplasts, PLB-associated thylakoids were swollen and failed to form grana stacks. By contrast, PLBs had cracks in their lattices incurt1cetioplasts. Our data provide evidence that CURT1A is required for pre-granal thylakoid assembly from PLB tubules during de-etiolation, while CURT1C contributes to the cubic crystal growth under darkness.
NUMT PARSER: automated identification and removal of nuclear mitochondrial pseudogenes (numts) for accurate mitochondrial genome reconstruction in Panthera
Authors: de Flamingh Alida , Rivera-Colón Angel G. , Gnoske Tom P. , Peterhans Julian C. Kerbis , Catchen Julian , Malhi Ripan S. , Roca Alfred L. 
Published: April 2022

AbstractNuclear mitochondrial pseudogenes (numts) may hinder the reconstruction of mtDNA genomes and affect the reliability of mtDNA datasets for phylogenetic and population genetic comparisons. Here, we present the program Numt Parser, which allows for the identification of DNA sequences that likely originate from numt pseudogene DNA. Sequencing reads are classified as originating from either numt or true cytoplasmic mitochondrial (cymt) DNA by direct comparison against cymt and numt reference sequences. Classified reads can then be parsed into cymt or numt datasets. We tested this program using whole genome shotgun-sequenced data from two ancient Cape lions (Panthera leo), because mtDNA is often the marker of choice for ancient DNA studies and the genus Panthera is known to have numt pseudogenes. Numt Parser decreased sequence disagreements that were likely due to numt pseudogene contamination and equalized read coverage across the mitogenome by removing reads that likely originated from numts. We compared the efficacy of Numt Parser to two other bioinformatic approaches that can be used to account for numt contamination. We found that Numt Parser outperformed approaches that rely only on read alignment or Basic Local Alignment Search Tool (BLAST) properties, and was effective at identifying sequences that likely originated from numts while having minimal impacts on the recovery of cymt reads. Numt Parser therefore improves the reconstruction of true mitogenomes, allowing for more accurate and robust biological inferences.
Physics informed neural networks for triple deck
Authors: Belkallouche Abderrahmane , Rezoug Tahar , Dala Laurent , Tan Kian 
Published: April 2022

Purpose
This paper aims to introduce physics-informed neural networks (PINN) applied to the two-dimensional steady-state laminar Navier–Stokes equations over a flat plate with roughness elements and specified local heating. The method bridges the gap between asymptotics theory and three-dimensional turbulent flow analyses, characterized by high costs in analysis setups and prohibitive computing times. The results indicate the possibility of using surface heating or wavy surface to control the incoming flow field.

Design/methodology/approach
The understanding of the flow control mechanism is normally caused by the unsteady interactions between the aircraft structure and the turbulent flows as well as some studies have shown, surface roughness can significantly influence the fluid dynamics by inducing perturbations in the velocity profile.

Findings
The description of the boundary-layer flow, based upon a triple-deck structure, shows how a wavy surface and a local surface heating generate an interaction between the inviscid region and the viscous region near the flat plate.

Originality/value
To the best of the authors’ knowledge, the presented approach is especially original in relation to the innovative concept of PINN as a solver of the asymptotic triple-deck method applied to the viscous–inviscid boundary layer interaction.
Institutional isomorphism and social media adoption – a study within the microenterprise context
Authors: Osakwe Christian Nedu , Ikhide Juliet E. 
Published: April 2022

PurposeUntil now, very little empirical research has focused on social media adoption behavior within relatively small firms, and fewer still on adoption drivers within the specific context of microentrepreneurs, including that of micro-retailers. The purpose of this manuscript is to contribute to the study on social media adoption at the firm level by focusing on the specific role of institutional pressures, as captured by coercive, mimetic and normative pressures, in the initial adoption of social media in the context of micro-retailers.Design/methodology/approachThis study, based on self-administered questionnaires, collected data from more than 200 micro-retailers in an emerging market and utilized the partial least squares modeling approach.FindingsFindings reveal that normative and mimetic (not coercive) pressures are critical to initial adoption. Additional analysis, though not directly the center of attention in the study, indicates that both coercive and normative pressures are critical to continued adoption, especially for retailers who currently use social media to promote their businesses.Originality/valueThis study represents one of the few attempts to extend the institutional theory to study social media adoption behavior in the firm. In addition, it is the first in the literature to extend the theory to social media adoption within the context of microenterprises, primarily micro-retailers, who form the significant majority in the world.
Dynamic capabilities and SME performance in the COVID-19 era: the moderating effect of digitalization
Authors: Martins Albert 
Published: April 2022

PurposeDue to the adverse impact of COVID-19 on businesses, this study investigates how dynamic capabilities could influence SME performance through digitalization as a moderator in an emerging market.Design/methodology/approachA quantitative research approach was adopted, where a cross-sectional survey design was employed to collect the data from 400 SME managers and supervisors in Ghana. Using Statistical Package for Social Science, the data was analyzed through descriptive statistics, correlation and hierarchical regression techniques.FindingsThe results reveal that the dynamic capabilities (sensing, seizing and transforming) distinctly positively affect SME performance. Furthermore, digitalization significantly augments the relationship between the three dynamic capabilities and SME performance. However, digitalization could only significantly moderate the relationship between transforming capability and SME performance.Practical implicationsThe findings imply that digitalization strategies (such as constant inclusion of digital analytics, digital operations, digital marketing and sales, digital ecosystem, and digital products and services) must be part of the transforming process to enhance the impact of the dynamic capabilities on SMEs performance.Originality/valueThis study contributes to the extant literature by establishing the effectiveness of digitalization as a moderator on the relationship between transforming capability and SME performance in the new world of COVID-19.
Enhancing industry-ready competence and skills through design thinking integration: evidence from a CLIL-based hospitality course
Authors: Wang Mei-jung Sebrina , Munoz Kyrie Eleison , Tham Aaron 
Published: April 2022

PurposeThe purpose of this paper is to argue for the merits of design thinking as an approach to develop a content and language integrated learning (CLIL) course in hospitality education. This argument is primed to address the dynamic and ever-changing landscape of the tourism and hospitality industry prompts educators to emphasize on learners developing industry-readiness outcomes through integrating innovative methods.Design/methodology/approachA quasi-experimental approach was used to examine how design thinking enhances industry-ready competencies. A two-phase self-administered survey on design thinking and added instructional design were facilitated at the beginning of the semester and subsequently close to the end of the semester. Follow-up interviews were also conducted to give more meaning on the matter.FindingsFindings of this study reveal that problem-solving, critical thinking, teamwork and communication skills were enhanced as a result of integrating two teaching components related to design thinking.Originality/valueThis paper articulates the important and valuable role of design thinking to hospitality curriculum and provides tangible outcomes to explain how such a technique may be mapped onto a CLIL course.
Free vibration analysis of fiber-metal laminated composite plates using differential, generalized and harmonic quadrature methods: experimental and numerical studies
Authors: Maraş Sinan , Yaman Mustafa 
Published: April 2022

PurposeThis study aims to demonstrate the numerical application of differential quadrature (DQ) methods and show the experimental application of free vibration analysis of fiber-metal laminated composite (FML) plates with various boundary conditions.Design/methodology/approachThe FMLs are hybrid structures consisting of fiber-reinforced polymer matrix composites such as carbon, glass, aramid and different metal sheets, and are currently widely used in the automobile, aircraft and aerospace industries. Thus, free vibration analysis of these hybrid materials is necessary for the design process. The governing equations of motion are derived based on the classical plate theory. The DQ, generalized DQ (GDQ) and harmonic DQ (HDQ) differential quadrature methods have been used to solve the governing equations of an FML composite plate numerically. The accuracy and convergence of the numerical model have been verified by comparing the results available in the published literature with the results obtained from these methods. Moreover, an experimental procedure has been performed in order to compare the results against those of the numerical methods.FindingsIt is noteworthy that a high degree of similarity and accuracy was observed between the numerical results obtained by the DQ methods and the experimental results. Thus, the present study validates the applicability of the DQ methods for designing the FML composite plates.Originality/valueIn this study, the advantages of the DQ methods have been demonstrated differently from previous studies on the vibration analysis of the FML plates.
Marigold Beads: focused innovation in a Zimbabwean co-operative
Authors: Moore Amy Fisher 
Published: April 2022

Learning outcomes
Following discussion and analysis of the case, students should be able to explore how an individual’s background affects his/her perspective on entrepreneurial opportunities; analyse leadership behaviours that support an innovation process; and understand that constraints can enhance innovation.

Case overview/Synopsis
This real-life case explores the main protagonist, Joni Brenner, an arts university professor at the University of Witwatersrand (Wits) in South Africa, and how over the past 10 years she worked with a group of co-operative beaders in Zimbabwe who developed hand loomed necklaces that were sold locally and internationally, placed in the African section of museum stores.The case provides an opportunity to explore the evolution of the Marigold product, the characteristics of an entrepreneur and how innovation can come from a very focused and constrained approach. Brenner’s involvement with the co-operative involved the supply of materials, design innovation and product sales. Innovation had come through focusing on the evolution of the core product, through different designs and colour combinations, learning through mistakes and through other artistic collaborations. The case concludes with Brenner questioning whether the innovation approach should be adapted to meet the needs of a potential new customer.

Complexity academic level
This case is appropriate for undergraduate, graduate and post-graduate, MBA and executive education students focusing on entrepreneurship, small business development and/or innovation.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 7: Management Science.
Optimizing product trials by eliciting flow states: the enabling roles of curiosity, openness and information valence
Authors: Lavoie Raymond , Main Kelley 
Published: April 2022

PurposeProduct trials are an effective way to influence consumer attitudes. While research has established several factors that influence whether consumers will try a product or not, it is less understood how marketers can optimize the trial experience itself. The purpose of this paper is to explore flow as an optimal state and the factors that give rise to it during a product trail.Design/methodology/approachThis research consists of three experimental studies in which people trial new music. This paper explores the ability of curiosity to optimize consumers’ flow experience during the trial and their attitudes toward the trialed product. This paper manipulates curiosity before the trial using information about the music (Study 1) and music previews (Study 3) and also demonstrates that curiosity is naturally elevated among those high in openness to experience (Study 2).FindingsThe results demonstrate that curiosity before a product trial fosters an optimal experience during the trial in the form of flow states, defined as an enjoyable state of full engagement, which in turn mediates more positive attitudes toward the trialed product. This paper demonstrates that curiosity can be evoked using product information or a preview of the content and can vary based on individual differences in openness to experience. The relationship between curiosity and flow is moderated by the valence of the information that is used to elicit curiosity, such that negative-valence information thwarts the relationship.Research limitations/implicationsWhile the studies conducted by the authors focus on the positive influence of curiosity in the trial of music, the effects may be different for other products. These studies are also limited to two different manipulations of curiosity.Practical implicationsThis research has implications for marketers, as it demonstrates the relevance of flow and how to enable it in product trials to optimize effectiveness. The manipulations also demonstrate how to manage the amount of information that is given to consumers before they trial a product.Originality/valueThis research reveals that flow states optimize the product trial experience. This research also advances the understanding of the relationship between curiosity and flow by moderating their relationship with the valence of information that elicits curiosity. The findings also broaden the relevance of curiosity and flow in marketing by demonstrating their benefits within product trials.
Does Michelin effect exist? An empirical study on the effects of Michelin stars
Authors: Bang Dohyung , Choi Kyuwan , Kim Alex Jiyoung 
Published: April 2022

Purpose
Receiving Michelin stars is considered an effective marketing tool and a prestigious certification in the restaurant industry. However, the direct effects of Michelin stars on restaurant performance remain unclear. To bridge this gap, this study aims to empirically validate the “Michelin effect” on the consumption values of restaurant patrons before and after receiving Michelin stars.

Design/methodology/approach
The data, collected from OpenTable, consist of over 160,000 reviews written for 218 restaurants, including 109 Michelin-starred (treatment group) and 109 nonstarred restaurants (control group). The authors measure perceived consumption value using the collected user-generated review data. The authors estimate fixed-effect difference-in-differences regressions to validate the Michelin effect.

Findings
Michelin stars enhance social, hedonic and service quality values, which are nonfunctional values. However, no significant effects on functional consumption values, such as economic, food quality and ambience quality values, are observed, even though Michelin stars are pure awards for kitchen performance.

Practical implications
Michelin stars can be an effective marketing tool for fine-dining restaurants because customers consider emotional and nonfunctional benefits such as hedonic and social values, which are more important than functional benefits such as food value. Furthermore, Michelin effects are heterogeneous depending on the number of stars awarded, price range and customers’ gastronomic involvement. These offer a strong rationale for monitoring social media, which may help managers better understand their customers and improve their performance.

Originality/value
This study extends the current literature on the Michelin effect by quantifying consumption values using user-generated review data. To the best of the authors’ knowledge, this study offers the first empirical evidence that directly validates the Michelin effect.