bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation
Authors: Gonzalez Pisfil Mariano , Nadelson Iliya , Bergner Brigitte , Rottmeier Sonja , Thomae Andreas W. , Dietzel Steffen 
Published: August 2022

AbstractStimulated emission depletion (STED) microscopy achieves super-resolution by exciting a diffraction-limited volume and then suppressing fluorescence in its outer parts by depletion. Multiple depletion lasers may introduce misalignment and bleaching. Hence, a single depletion wavelength is preferable for multi-color analyses. However, this limits the number of usable spectral channels. Using cultured cells, common staining protocols, and commercially available fluorochromes and microscopes we exploit that the number of fluorochromes in STED or confocal microscopy can be increased by phasor based fluorescence lifetime separation of two dyes with similar emission spectra but different fluorescent lifetimes. In our multi-color FLIM-STED approach two fluorochromes in the near red (exc. 594 nm, em. 600–630) and two in the far red channel (633/641–680), supplemented by a single further redshifted fluorochrome (670/701–750) were all depleted with a single laser at 775 nm thus avoiding potential alignment issues. Generally, this approach doubles the number of fully distinguishable colors in laser scanning microscopy. We provide evidence that eight color FLIM-STED with a single depletion laser would be possible if suitable fluorochromes were identified and we confirm that a fluorochrome may have different lifetimes depending on the molecules to which it is coupled.
Individual differences in white matter microstructure of the face processing brain network are more differentiated from global fibers with increasing ability
Authors: Liu Xinyang , Geiger Mattis , Zhou Changsong , Hildebrandt Andrea 
Published: August 2022

AbstractFace processing—a crucial social ability—is known to be carried out in multiple dedicated brain regions which form a distinguishable network. Previous studies on face processing mainly targeted the functionality of face-selective grey matter regions. Thus, it is still partly unknown how white matter structures within the face network underpins abilities in this domain. Furthermore, how relevant abilities modulate the relationship between face-selective and global fibers remains to be discovered. Here, we aimed to fill these gaps by exploring linear and non-linear associations between microstructural properties of brain fibers (namely fractional anisotropy, mean diffusivity, axial and radial diffusivity) and face processing ability. Using structural equation modeling, we found significant linear associations between specific properties of fibers in the face network and face processing ability in a young adult sample (N = 1025) of the Human Connectome Project. Furthermore, individual differences in the microstructural properties of the face processing brain system tended toward stronger differentiation from global brain fibers with increasing ability. This is especially the case in the low or high ability range. Overall, our study provides novel evidence for ability-dependent specialization of brain structure in the face network, which promotes a comprehensive understanding of face selectivity.
Unusual behaviour of the spin-phonon coupling in the quasi-one-dimensional antiferromagnet RbCoCl3
Authors: Cottam M. G. , Lockwood D. J. 
Published: August 2022

AbstractWe present an experimental and theoretical study for the lattice vibrational (phonon) modes in the quasi-one-dimensional (or chain-like) antiferromagnet RbCoCl3 at low temperatures both above and below the two different magnetic phase transitions. Clear evidence is found for the role of spin-phonon interactions in providing a temperature-dependent contribution for the frequencies of the E1g and E2g symmetry phonons that occur with frequencies comparable to those of the spin wave excitations (magnons) in this compound. The behaviour in RbCoCl3, as studied here by Raman scattering experiments, is quite different from that typically observed in rutile-structure antiferromagnets where the spin-phonon coupling has been well characterized. The theory is modified to take account of the strong Ising-like component in the spin Hamiltonian. This enables the spin-phonon coupling parameters to be deduced, with the analysis also revealing the onset of an extra frequency shift for the phonons below the transition temperature TN1 = 28 K associated with magnetic ordering along the Co chains.
Safety and feasibility of radiofrequency ablation using bipolar electrodes for aldosterone-producing adenoma: a multicentric prospective clinical study
Authors: Oguro Sota , Morimoto Ryo , Seiji Kazumasa , Ota Hideki , Kinoshita Tomo , Kawabata Masahiro , Ono Yoshikiyo , Omata Kei , Tezuka Yuta , Satoh Fumitoshi , Ito Sadayoshi , Moriya Nobukazu , Matsui Seishi , Nishikawa Tetsuo , Omura Masao , Nakai Kazuki , Nakatsuka Seishi , Kurihara Isao , Miyashita Kazutoshi , Koda Wataru , Minami Tetsuya , Takeda Yoshiyu , Kometani Mitsuhiro , Oki Yutaka , Oishi Toshihiro , Ushio Takasuke , Goshima Satoshi , Takase Kei 
Published: August 2022

AbstractEvaluation of feasibility and safety of percutaneous radiofrequency ablation using bipolar radiofrequency devices in a prospective multicenter cohort of patients with benign aldosterone-producing adenoma. A total of five institutions participated. CT-guided percutaneous RFA was performed for patients diagnosed as APA. The safety of the procedure was evaluated using the Common Terminology Criteria for Adverse Events. During the 84-day follow-up period, serial changes in plasma aldosterone concentration and plasma renin activity were measured. The percentage of patients with normalized hormonal activity after the procedure, was calculated with 95% confidence intervals. Forty patients were enrolled, and two patients were excluded for cerebral hemorrhage and no safe puncture root. In another patients, RFA was tried, but an intraprocedural intercostal arterial injury occurred. Consequently, RFA was completed in thirty-seven patients (20 men, 17 women; mean age, 50.4 ± 10.0 year). The tumor size was 14.8 ± 3.8 mm. The treatment success rate of the ablation was 94.6% (35/37), and a 2nd session was performed in 2.7% (1/37) patients. Grade 4 adverse events were observed in 4 out of 38 sessions (10.5%). The normalization of plasma aldosterone concentration or aldosterone-renin ratio was 86.5% (72.0–94.1: 95% confidence interval) on day 84. Percutaneous CT-guided RFA for APA using a bipolar radiofrequency system was safe and feasible with clinical success rate of 86.5% on day 84.
The effects of vitamin B12 supplementation on metabolic profile of patients with non-alcoholic fatty liver disease: a randomized controlled trial
Authors: Talari Hamid Reza , Molaqanbari Mohamad Reza , Mokfi Milad , Taghizadeh Mohsen , Bahmani Fereshteh , Tabatabaei Seyed Mohammad Hossein , Sharifi Nasrin 
Published: August 2022

AbstractThe present study is the first effort to evaluate the effects of vitamin B12 supplementation on the serum level of liver enzymes, homocysteine, grade of hepatic steatosis, and metabolic profiles in patients with non-alcoholic fatty liver disease (NAFLD). Forty patients with NAFLD were enrolled in a double-blind placebo-controlled trial to receive either one oral tablet of vitamin B12 (1000 µg cyanocobalamin) or a placebo per day for 12 weeks. We investigated serum levels of homocysteine, aminotransferases, fasting blood glucose (FBG), lipids, malondialdehyde (MDA), and homeostasis model assessment of insulin resistance (HOMA-IR). The grade of liver steatosis and fibrosis was measured by real-time 2-dimensional shear wave elastography. Vitamin B12 supplementation significantly decreased serum levels of homocysteine compared to placebo (medians: − 2.1 vs. − 0.003 µmol/l; P = 0.038). Although serum alanine transaminase (ALT) in the vitamin B12 group decreased significantly, this change did not reach a significant level compared to the placebo group (medians: − 7.0 vs. 0.0 IU/l; P > 0.05). Despite the significant within-group decrease in FBG, MDA, and liver steatosis in the vitamin B12 group, between-group comparisons did not reveal any significant difference. Vitamin B12 supplementation might decrease serum levels of homocysteine in patients with NAFLD. The fasting blood glucose and serum levels of MDA were significantly improved in the trial group who received vitamin B12. However, these changes did not reach a significant level compared to the placebo group. In this respect, further studies with larger sample sizes, different doses, and types of vitamin B12 will reveal additional evidence.Trial Registration: At http://irct.ir/ as IRCT20120718010333N5 on December 25, 2019.
Octopamine signaling via OctαR is essential for a well-orchestrated climbing performance of adult Drosophila melanogaster
Authors: El-Kholy Samar Ezzat , Afifi Basma , El-Husseiny Iman , Seif Amal 
Published: August 2022

AbstractThe biogenic amine octopamine (OA) orchestrates many behavioural processes in insects. OA mediates its function by binding to OA receptors belonging to the G protein-coupled receptors superfamily. Despite the potential relevance of OA, our knowledge about the role of each octopaminergic receptor and how signalling through these receptors controls locomotion still limited. In this study, RNA interference (RNAi) was used to knockdown each OA receptor type in almost all Drosophila melanogaster tissues using a tubP-GAL4 driver to investigate the loss of which receptor affects the climbing ability of adult flies. The results demonstrated that although all octopaminergic receptors are involved in normal negative geotaxis but OctαR-deficient flies had impaired climbing ability more than those deficient in other OA receptors. Mutation in OA receptors coding genes develop weak climbing behaviour. Directing knockdown of octαR either in muscular system or nervous system or when more specifically restricted to motor and gravity sensing neurons result in similar impaired climbing phenotype, indicating that within Drosophila legs, OA through OctαR orchestrated the nervous system control and muscular tissue responses. OctαR-deficient adult males showed morphometric changes in the length and width of leg parts. Leg parts morphometric changes were also observed in Drosophila mutant in OctαR. Transmission electron microscopy revealed that the leg muscles OctαR-deficient flies have severe ultrastructural changes compared to those of control flies indicating the role played by OctαR signalling in normal muscular system development. The severe impairment in the climbing performance of OctαR-deficient flies correlates well with the completely distorted leg muscle ultrastructure in these flies. Taken together, we could conclude that OA via OctαR plays an important multifactorial role in controlling locomotor activity of Drosophila.
Pedigree reconstruction and genetic analysis of major ornamental characters of ornamental crabapple (Malus spp.) based on paternity analysis
Authors: Rong Hao , Huang Bin , Han Xin , Wu Kai , Xu Meng , Zhang Wangxiang , Yang Feng , Xu Li-an 
Published: August 2022

AbstractOrnamental crabapple is an important woody ornamental plant in the Northern Hemisphere. Its flowers, fruits, leaves and tree habit are all important ornamental characters. As there has been no research on the selection of superior parents and phenotypic variation, new varieties of ornamental crabapple are mainly selected from open-pollination progeny. In order to explore the transmission rule of ornamental traits between parents and offspring of crabapple, and to provide a basis for the selection of hybrid parents for directional breeding, 14 pairs of SSR markers were used in this study for paternity analysis of 384 offspring from 4 female parents crossed with 91 candidate male parents. And 273 offspring (71.1%) were matched with only the father at a 95% strict confidence level. We reconstructed 7 full-sib families (number of progeny ≥ 10) on the basis of the paternity analysis results. Genetic analysis of characters in the full-sib families revealed that green leaves and white flowers were dominant traits. All the hybrid offspring from the white flower (♀) × non-white flower (♂) cross produced white flowers, while 7.04% produced non-white flowers when both parents had white flowers. The results showed that white flowers might be a dominant qualitative trait in crabapple, while the depth of red was a quantitative trait. The genetic characteristics of green and non-green leaves and the depth of red of the peel were similar to flower color. Compared with the upright and spreading traits, the weeping trait was recessive. Some progeny showed an earlier blooming period, indicating the possibility of breeding for blooming period. Our findings are important for parent screening and improving the breeding efficiency of new varieties in ornamental crabapple hybridization.
Utilising low-cost, easy-to-use microscopy techniques for early peritonitis infection screening in peritoneal dialysis patients
Authors: Buckup Mark , Kaneda Janelle M. , Birk Alisha M. , Glockner Eleanor , Venook Ross , Jain Aditya , Sharma Shuchita , Wong Cynthia , Sutha Ken 
Published: August 2022

AbstractPeritoneal dialysis (PD) patients are at high risk for peritonitis, an infection of the peritoneum that affects 13% of PD users annually. Relying on subjective peritonitis symptoms results in delayed treatment, leading to high hospitalisation costs, peritoneal scarring, and premature transition to haemodialysis. We have developed and tested a low-cost, easy-to-use technology that uses microscopy and image analysis to screen for peritonitis across the effluent drain tube. Compared to other technologies, our prototype is made from off-the-shelf, low-cost materials. It can be set up quickly and key stakeholders believe it can improve the overall PD experience. We demonstrate that our prototype classifies infection-indicating and healthy white blood cell levels in clinically collected patient effluent with 94% accuracy. Integration of our technology into PD setups as a screening tool for peritonitis would enable earlier physician notification, allowing for prompt diagnosis and treatment to prevent hospitalisations, reduce scarring, and increase PD longevity. Our findings demonstrate the versatility of microscopy and image analysis for infection screening and are a proof of principle for their future applications in health care.
Prolonged insulin-induced hypoglycaemia reduces ß-cell activity rather than number in pancreatic islets in non-diabetic rats
Authors: Jensen Vivi F. H. , Mølck Anne-Marie , Nowak Jette , Fels Johannes J. , Lykkesfeldt Jens , Bøgh Ingrid B. 
Published: August 2022

AbstractPancreatic β-cells have an extraordinary ability to adapt to acute fluctuations in glucose levels by rapid changing insulin production to meet metabolic needs. Although acute changes have been characterised, effects of prolonged metabolic stress on β-cell dynamics are still unclear. Here, the aim was to investigate pancreatic β-cell dynamics and function during and after prolonged hypoglycaemia. Hypoglycaemia was induced in male and female rats by infusion of human insulin for 8 weeks, followed by a 4-week infusion-free recovery period. Animals were euthanized after 4 or 8 weeks of infusion, and either 2 days and 4 weeks after infusion-stop. Total volumes of pancreatic islets and β-cell nuclei, islet insulin and glucagon content, and plasma c-peptide levels were quantified. Prolonged hypoglycaemia reduced c-peptide levels, islet volume and almost depleted islet insulin. Relative β-cell nuclei: total pancreas volume decreased, while being unchanged relative to islet volume. Glucagon: total pancreas volume decreased during hypoglycaemia, whereas glucagon: islet volume increased. Within two days after infusion-stop, plasma glucose and c-peptide levels normalised and all remaining parameters were fully reversed after 4 weeks. In conclusion, our findings indicate that prolonged hypoglycaemia inactivates β-cells, which can rapidly be reactivated when needed, demonstrating the high plasticity of β-cells even following prolonged suppression.
Influence of DOM and its subfractions on the mobilization of heavy metals in rhizosphere soil solution
Authors: Lian Meihua , Wang Jun , Ma Yangyang , Li Jiahui , Zeng Xiangfeng 
Published: August 2022

AbstractLong-term industrial pollution, wastewater irrigation, and fertilizer application are known factors that can contribute to the contamination of heavy metals (HMs) in agricultural soil. In addition, dissolved organic matter (DOM) plays key roles in the migration and fate of HMs in soil. This study investigated the effects of amending exogenous DOM extracted from chicken manure (DOMc), humus soil (DOMs), rice husk (DOMr), and its sub-fractions on the mobilization and bio-uptake of Cd, Zn, and Pb. The results suggested that the exogenous DOM facilitate the dissolution of HMs in rhizosphere soil, and the maximum solubility of Zn, Cd, and Pb were 1264.5, 121.3, and 215.7 μg L−1, respectively. Moreover, the proportion of Zn-DOM and Cd-DOM increased as the DOM concentration increased, and the highest proportions were 97.5% and 86.9%. However, the proportion of Pb-DOM was stable at > 99% in all treatments. In addition, the proportion of hydrophilic acid (Hy) and Pb/Cd in the rhizosphere soil solution were 17.5% and 8.3%, respectively. This finding suggested that the Hy-metals complex has a vital influence on the mobilization of metals, besides its complexation with fulvic acid and humic acid. Furthermore, the elevated DOM addition contributed to an increment of HMs uptake by Sedum alfredii, in the following order, DOMc > DOMs > DOMr. This study can provide valuable insights to enhance the development of phytoremediation technologies and farmland manipulation. Since the risk that exogenous DOM would increase the uptake of HMs by crops, it is also needed to evaluate this case from an agricultural management perspective.
Influence of posterior tibial slope on sagittal knee alignment with comparing contralateral knees of anterior cruciate ligament injured patients to healthy knees
Authors: Hiranaka Yoshiaki , Muratsu Hirotsugu , Tsubosaka Masanori , Matsumoto Tomoyuki , Maruo Akihiro , Miya Hidetoshi , Kuroda Ryosuke , Matsushita Takehiko 
Published: August 2022

AbstractPosterior tibial slope (PTS) has been known to contribute to anterior–posterior knee stability and play an essential biomechanical role in knee kinematics. This study aimed to investigate the effect of PTS on single-leg standing sagittal knee alignment of the intact knee. This study included 100 patients with unilateral ACL injury knee (ACL injury group, 53 patients) or with the normal knee (control group, 47 patients). The single-leg standing sagittal alignment of the unaffected knees of the ACL injury group and normal knees of the control group were assessed radiographically with the following parameters: knee extension angle (EXT), PTS, PTS to the horizontal line (PTS-H), femoral shaft anterior tilt to the vertical axis (FAT), and tibial shaft anterior tilt to the vertical axis (TAT). PTS was negatively correlated with EXT and positively correlated with TAT. EXT was significantly larger in the ACL injury group, whereas TAT was smaller in the ACL injury group. Patients with larger PTS tend to stand with a higher knee flexion angle by tilting the tibia anteriorly, possibly reducing tibial shear force. Patients with ACL injury tend to stand with larger EXT, i.e., there is less preventive alignment to minimize the tibial shear force.
Receiver aperture and multipath effects on power loss and modal crosstalk in a THz wireless link using orbital-angular-momentum multiplexing
Authors: Su Xinzhou , Zhang Runzhou , Zhao Zhe , Song Hao , Minoofar Amir , Hu Nanzhe , Zhou Huibin , Zou Kaiheng , Pang Kai , Song Haoqian , Lynn Brittany , Zach Shlomo , Tur Moshe , Molisch Andreas F. , Sasaki Hirofumi , Lee Doohwan , Willner Alan E. 
Published: August 2022

AbstractThe channel capacity of terahertz (THz) wireless communications can be increased by multiplexing multiple orthogonal data-carrying orbital-angular-momentum (OAM) beams. In THz links using OAM multiplexing (e.g., Laguerre-Gaussian $${\mathrm{LG}}_{ \ell,p}$$

LG

ℓ
,
p

beams with p = 0), the system performance might degrade due to limited receiver aperture size and multipath effects. A limited-size aperture can truncate the received beam profile along the radial direction. In addition, due to beam divergence, part of the beam might interact with reflectors in the environment, causing the signal to reflect and interfere at the receiver with the directly propagating part of the beam; this is known as the multipath effect. In this paper, we simulate and analyze the impact of both effects on the equality of the THz OAM link by considering a full two-dimensional (2-D) LG modal set. The simulation results show (i) a limited-size receiver aperture can induce power loss and modal power coupling mainly to LG modes with the same ℓ but p > 0 for directly propagated OAM beams; (ii) the multipath effect can induce modal power coupling across multiple 2-D LG modes, which leads to inter-channel coupling among the different channels in an OAM multiplexed link; (iii) the interference between the reflected and direct beams can induce intra-channel coupling between the received signals from the reflected and direct beams; and (iv) beams with a higher OAM order (e.g., from ± 1 to ± 5) or a lower carrier frequency (e.g., from 0.1 to 1 THz) experience larger intra- and inter-channel coupling. The intra- and inter-channel coupling in an OAM-multiplexed THz link can degrade the signal-to-noise ratio (SNR) and induce SNR penalty when compared to a single-channel system.
Automated diagnosis of optical coherence tomography imaging on plaque vulnerability and its relation to clinical outcomes in coronary artery disease
Authors: Niioka Hirohiko , Kume Teruyoshi , Kubo Takashi , Soeda Tsunenari , Watanabe Makoto , Yamada Ryotaro , Sakata Yasushi , Miyamoto Yoshihiro , Wang Bowen , Nagahara Hajime , Miyake Jun , Akasaka Takashi , Saito Yoshihiko , Uemura Shiro 
Published: August 2022

AbstractThis study sought to develop a deep learning-based diagnostic algorithm for plaque vulnerability by analyzing intravascular optical coherence tomography (OCT) images and to investigate the relation between AI-plaque vulnerability and clinical outcomes in patients with coronary artery disease (CAD). A total of 1791 study patients who underwent OCT examinations were recruited from a multicenter clinical database, and the OCT images were first labeled as either normal, a stable plaque, or a vulnerable plaque by expert cardiologists. A DenseNet-121-based deep learning algorithm for plaque characterization was developed by training with 44,947 prelabeled OCT images, and demonstrated excellent differentiation among normal, stable plaques, and vulnerable plaques. Patients who were diagnosed with vulnerable plaques by the algorithm had a significantly higher rate of both events from the OCT-observed segments and clinical events than the patients with normal and stable plaque (log-rank p < 0.001). On the multivariate logistic regression analyses, the OCT diagnosis of a vulnerable plaque by the algorithm was independently associated with both types of events (p = 0.047 and p < 0.001, respectively). The AI analysis of intracoronary OCT imaging can assist cardiologists in diagnosing plaque vulnerability and identifying CAD patients with a high probability of occurrence of future clinical events.