bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation and quantification of a UPLC-MS/MS method for the simultaneous determination of quinocetone and its main metabolites (3-methylquinoxaline-2-carboxylic acid and dedioxoquinenone) in aquatic products
Authors: Tian Xiuhui , Han Dianfeng , Cui Yanmei , Ren Lihua , Jiang Fang , Huang Hui , Gong Xianghong , Xue Jinglin , Li Jiawei , Liu Huihui , Xu Yingjiang , Luo Xiaojun , Liu Xiaojing , Zhang Xiuzhen 
Published: March 2022

Abstract
A sensitive and validated method for determining quinocetone and its main metabolites (3-methylquinoxaline-2-carboxylic acid and dedioxoquinenone) was established in aquatic products using ultra-high-performance liquid chromatography-tandem spectrometry (UHPLC-MS/MS). Samples were extracted with 2.0 mol L−1 hydrochloric acid, then purified on MAX columns. After extraction and purification, the supernatant was evaporated to dry nearly under a gentle stream of nitrogen at 40 °C. Formic acid-acetonitrile-water (0.1/30/70, v/v/v) was adjusted to 1.00 mL final volume. An aliquot (10 μL) was injected into the C18 column for separation with the mobile phase of acetonitrile and 0.5% formic acid in water at 0.25 mL min−1. Calibration curves were linear ranged from 10.00 ng mL−1 to 200.0 ng mL−1 for quinocetone and 3-methylquinoxaline-2-carboxylic acid, and 20.00 ng mL−1 to 400.0 ng mL−1 for dedioxoquinenone. Mean recoveries were 70%–89%, 73%–83% and 72%–84%, respectively. The limit of detection (LOD) was 1.00 μg kg−1, 1.00 μg kg−1 and 2.00 μg kg−1, and quantification (LOQ) were 2.00 μg kg−1, 2.00 μg kg−1 and 4.00 μg kg−1 for quinocetone, 3-methylquinoxaline-2-carboxylic acid, and dedioxoquinenone. Based on the method above, the analytes were determined in Apostichopus japonicus, three fishes (including Ctenopharyngodon idellus, Crucian carp and Oreochromis mossambicus), Penaeus vannamei, Penaeus chinensis, and Chlamys farreri. The method shows good sensitivity, linearity, precision, and accuracy. In short, the proposed method was reliable for the determination of quinocetone, 3-methylquinoxaline-2-carboxylic acid, and dedioxoquinenone in aquatic products.
Heart rate (HR) and heart rate variability (HRV) response to inhaled N, N-dimethyltryptamine (N, N-DMT): A case report
Authors: Lawrence David Wyndham 
Published: March 2022

Abstract

Background
N, N-Dimethyltryptamine (N, N-DMT) is a naturally occurring tryptamine-based alkaloid and classic psychedelic. The potent psychoactive properties of N, N-DMT are mediated by serotonin 2A (5-HT2A) receptor agonism. There are limited investigations into the physiologic effects of inhaled N, N-DMT, despite this being one of the most common routes of administration. Heart rate variability (HRV) is an emergent non-invasive marker of cardiac autonomic nervous system (ANS) regulation and reductions in HRV have been negatively associated with multiple health outcomes.

Methods
A case is described with a brief review of supportive findings.

Results
Heart rate (HR) and HRV responses following three naturalistic inhaled N, N-DMT experiences were documented in a healthy 31-year-old male. Acute transient elevations in HR immediately following N, N-DMT administration and a pronounced elevation in the average daily HRV one-day following the experiences were observed. The duration of experience shortened across the three experiences despite similar N, N-DMT doses.

Conclusion
These findings offer preliminary evidence into the effect of inhaled N, N-DMT on HR and HRV. Given the emergent understanding of reductions in HRV as a hazardous marker of stress, psychiatric conditions, and mental health outcomes, further research into the effects of psychedelics on HRV is warranted.
Strategi Kebijakan Lingkungan Program Citarum Harum Di Kabupaten Bandung
Authors: Andri Yayan , Aziz Yaya Mulyana Abdul 
Published: March 2022

So great is the loss suffered due to the contamination of the Citarum River for decades. Starting from the damage in the Citarum Hulu watershed (DAS) to the pollution of industrial waste and domestic waste. Not to mention, flood disasters due to overflowing rivers and sedimentation that often occur in the Bandung Basin, especially Baleendah and Dayeuhkolot. The research method used in this study is descriptive analysis with the type of research being qualitative. The qualitative approach was chosen with the consideration that this method is expected to obtain real data and be able to examine research problems in depth so that the expected results can be obtained. The use of this qualitative research approach is felt to be very appropriate in the study of public administration. The results of the research is an appropriate step in controlling the damage and pollution of watersheds. One form of government policy strategy by issuing Presidential Decree no. 15 of 2018 concerning the Acceleration of Pollution Control and Damage to the Citarum River Basin. With the existence of the Citarum Harum Program policy, it is an environmental policy strategy by observing, controlling problematic environmental conditions and even having to immediately restore the image of the Citarum River which was once tarnished in the eyes of the world as the dirtiest river in the world. With a collaborative step that is able to cover the Siliwangi Kodam III Sector which is divided into 22 Task Forces along the banks of the Citarum River, this is a strategy in restoring the problems that occur in the Citarum River. This policy program will end in 2025, so there must be a follow-up to the next program as well as a success evaluation program so that it does not repeat itself like the previous policy programs which were considered less successful and cost a large amount.
Characteristics of Triple Seronegative Myasthenia Gravis: A Single Center Experience
Authors: Morena Jonathan , Jiang Benjamin , Freimer Miriam , Hoyle Joseph , Elsheikh Bakri , Arnold David , LoRusso Samantha 
Published: March 2022

Background: There is variability in the literature regarding the characteristics of triple seronegative myasthenia gravis (SNMG) patients. Most studies were performed before LRP4 antibodies were discovered, and characterizations of triple seronegative patients are lacking in the literature.
Methods: We retrospectively investigated patients diagnosed with myasthenia gravis (MG) at Ohio State University from 2009 to 2019. Triple SNMG was defined by a history and examination that was consistent with MG and positive SFEMG, RNS or edrophonium testing, but negative serology for AChR, MUSK, and LRP4 antibodies.
Results: A total of 210 AChR+, 9 MuSK+, 6 LRP4+, 9 double SNMG, and 21 triple SNMG patients were reviewed. Triple SNMG patients required significantly fewer immunosuppressive agents compared with AChR+ patients (p=0.0001) and a trend towards a less frequent history of hospitalizations, myasthenic crises and intubations compared to all antibody positive groups. Triple SNMG patients had a significantly higher frequency of ocular disease (33%) compared to AChR+ patients (13%) (p=0.0250). One triple and one double SNMG patient had thymic hyperplasia and improved after thymectomy. 11 triple SNMG patients had negative genetic testing for CMS.
Conclusion: Our results further elucidate the clinical characteristics of triple SNMG, which include the predominance for ocular disease and a less severe disease course. Although likely rare, investigation for thymic pathology should be a consideration even in SNMG, and thymectomy should be considered when there is thymic pathology. We did not find alternate diagnoses in SNMG patients and thus ancillary testing should be considered in carefully selected patients for cost-effective care.
Quality of Black Bean Seeds Submitted to Pre-Harvest Desiccation by Different Active Principles and Application Times
Authors: Simionatto Tatieli , D’Agostini Contreiras Rodrigues Adriana Paula , Dalla Costa Daniela Aparecida , Brum de Bortolli Betania , Pereira Silvia Rahe , Ferreira Coelho Aline 
Published: March 2022

O objetivo deste estudo foi avaliar o momento ideal de dessecação e de que forma diferentes princípios ativos influenciam a produtividade e a qualidade física e fisiológica de sementes de feijão-preto, cultivar BRS Esteio. O experimento foi conduzido na safra 2018/2019, no município de Pato Branco, Paraná. A semeadura foi realizada no dia 07/11/2018 e o delineamento experimental adotado foi de blocos ao acaso (DBA), com quatro repetições. Os tratamentos foram arranjados em esquema fatorial 4 x 4 (quatro herbicidas x quatro épocas de aplicação). A aplicação dos dessecantes foi realizada quando a cultura apresentou 60%, 70%, 80% e 90% das vagens no estágio de maturação de campo. E os princípios ativos e doses utilizadas foram: diquate (400 g de i.a. ha-1), glufosinato – sal de amônio (400 g de i.a. ha-1 + óleo mineral), saflufenacil (49 g de i.a. ha-1) e glifosato potássico (1.240 g de i.a. ha-1). As avaliações pós-colheita foram as seguintes: peso de mil sementes (g), produtividade (Kg ha-1), teste de germinação (%), teste de envelhecimento acelerado (%) e comprimento de parte aérea e raiz de plântulas (cm). Independente do princípio ativo utilizado, visando a produção de sementes, a dessecação da cultivar de feijão-preto BRS Esteio, deve ser realizada quando 60% das vagens se encontram no estágio de maturação de campo. Além disso, as sementes dessecadas com glufosinato – sal de amônio, resultaram em sementes mais pesadas e em plântulas mais vigorosas, contrariamente ao observado para o glifosato potássico que reduziu o vigor de plântulas.

Palavras-chave: Phaseolus vulgaris L. Herbicides. Maturation. Physiological Quality. Seed Technology.
Acute effects of energy drink consumption on left and right ventricular function – a 2-dimensional speckle tracking echocardiographic study
Authors: Tulunay Kaya Cansin , Gerede Demet Menekse , Akhundova Javidan 
Published: March 2022

Aim Energy drinks (ED) contain high levels of caffeine and taurine and are associated with several cardiovascular effects. We investigated acute effects of consuming low caffeine and taurine content ED on left ventricular (LV) and right ventricular (RV) function assessed by conventional and two-dimensional speckle tracking echocardiography.Material and methods In this crossover study, 34 healthy adults, age 19–48 yrs, drank an ED containing 53.25 milligrams of caffeine, 284 mg of taurine, or an equal volume of control drink (CD) on two separate sessions, 7–10 days apart. Standard echocardiographic and speckle tracking imaging were performed before and 60 min after consumption of the study beverages.Results Compared to CD, ED caused a significant increase in tricuspid annular plane systolic excursion (p=0.04) and RV systolic wave velocity (p=0.01) with no effect on global longitudinal strain when compared to CD. LV systolic function was not altered, but mitral early diastolic velocity by tissue Doppler imaging was significantly higher (p=0.031), and early diastolic strain rate, as measured by speckle tracking echocardiography, was significantly lower (p=0.022).Conclusion Reduced caffeine and taurine content ED does not affect LV systolic function, but increases RV longitudinal contractility and improves LV early diastolic filling.
A cross-sectional study of thyroid profile, Anti-TPO and iron levels with uric acid of melasma cases in tertiary hospital, Bhopal
Authors: Yadav Gayatri , Dutta Jusmita , Thambrakar Seema 
Published: March 2022

Melasma, also called ‘chloasma’, is a common skin condition of adults in which light to dark brown or grayish pigmentation develops, mainly on the face. Although it can affect both genders and any race, it is more common in women. Melasma is an acquired pigmentary disorder described as symmetrical blotchy or splotchy hyperpigmented macules and patches. The aim of this study was to investigate thyroid profile with anti TPO and iron, uric acid level, in male and female patients. It is a cross-sectional observational study done on 76 females and 25 male who were diagnosed with melasma and thyroid profile with anti TPO, serum iron and uric acid levels were measured. In our study females were more affected then males. In females Mean ± SD value of serum TSH (5.1±2.51) and anti-TPO (11.2±3.1) levels were increased when compared to males(4.9±2.54) and (9.2±1.9). Serum iron value was low in females (83.7±30) than males (90±29). All biochemical parameter were not significant but anti TPO and FT3 showed significant (P&#60;0.001) in female with compared to male. Serum uric acid (3.56±0.77) and serum iron (83.7±30) Mean± SD value was low in melasma females than males which is not significant. Females Mean±SD value of T3 (1.30±0.62) and T4(6.8±1.9), FT4 (1.2±0.44) were non significance showed than compared to males and not significant to males T3 (1.33±0.66) and T4(7.1±1.6), FT4 (1.3±0.67) respectively. Melasma is more severe in females than males. Thyroid profile is not the accurate parameter but anti TPO, and iron level are predictive marker of melasma.
Assessment of serum calcium and magnesium levels in subjects with pre-menstrual syndrome
Authors: Chandna Ashish , Sushma B J  , Lalwani Roshni 
Published: March 2022

Women's health is one of the main goals of social and economic development of societies; therefore, problems and diseases compromising women's physical and mental health, such as premenstrual syndrome (PMS), are among health priorities.To estimate and compare the serum levels of calcium and magnesium in subjects with pre-menstrual syndrome and subjects without premenstrual syndrome during pre-and post-menstrual phases.: Healthy women (intern students, hospital staff, non-teaching staff, class 3 and class 4 staff) in the age group of 18-26 years having regular menstrual cycles for the past 6 months were included based on inclusion and exclusion criteria. PMS was diagnosed based on ACOG criteria. 5 mL of venous blood sample was collected in red stoppered plain tube and used for estimation of serum calcium and magnesium levels during premenstrual phase of menstrual cycle. We found that there was no significant difference in the serum calcium levels between cases and controls, but there was significant difference in serum magnesium levels between the cases and controls.Several factors like culture, socioeconomic status, cigarette smoking, alcohol consumption, exercise, dietary habits, age of menarche etc. have also been implicated. Prolonged stress exposure is believed to lead to persistent malfunctions of neuroendocrine system and cause PMS. Lack of micronutrients and certain hormonal agents and are also implicated in the development of PMS. We strongly recommend Lifestyle modifications which include mind-body approaches, aerobic exercise, and supplementation with vitamins, minerals, and complex carbohydrates. Early nutritional supplementation of with the diet rich in these minerals will be effective as preventive and therapeutic strategy.
Role of biochemical markers in rheumatoid arthritis
Authors: Mahmood Shaikh , Sadaf Samreen , Kausar Ruksana , Anees Syyeda 
Published: March 2022

Rheumatoid arthritis is the most common chronic, progressive systemic inflammatory arthritis of unknown cause. It affects approximately 1-2% of the population worldwide. It is a form of autoimmune disease principally synovial tissues. As the pathophysiology involves affecting cell mediated immune response, adenosine deaminase may have a role in rheumatoid arthritis. As it involves bones and joints, it is likely that it alters the levels of Serum Calcium, Phosphorus and Magnesium as well as Alkaline Phosphatase which may be helpful in diagnosis or management of the disease. 1. To evaluate the role of serum adenosine deaminase (ADA) as a marker of cell mediated immunity in Rheumatoid Arthritis. 2. To estimate serum alkaline phosphatase, calcium, phosphorus and magnesium as prognostic markers of Rheumatoid arthritisA cross-sectional, case control study with convenient sampling was undertaken after Institutional Ethical Clearance & informed consent. 30 patients of Rheumatoid Arthritis presenting to the orthopaedic outpatient department of Owaisi Hospital and Research Centre, Hyderabad were taken as cases along with an equal number of age and sex matched controls from January 2021 to June 2021. ADA levels were estimated by colorimetric assay on ErbaChem 7 semiautoanalyser. Serum Alkaline phosphatase, Calcium, Phosphorus and Magnesium were estimated on Cobas C311 autoanalyzer. There was a significant increase in levels of Adenosine Deaminase, Alkaline Phosphatase and Serum Phosphorus, while the levels of Calcium and Magnesium were significantly reduced in rheumatoid arthritisADA as a marker of cell mediated immunity reflects monocyte / macrophage activity and was increased in our study suggesting immunological and inflammatory process in the pathogenesis of Rheumatoid Arthritis (R.A). It can be used as a non invasive marker of chronic inflammation and may provide additional information about disease prognosis.Increased levels of Alkaline Phosphatase and Phosphorus associated with low Calcium and Magnesium suggest increased bone resorption in R. A and are useful as markers of disease activity.
Nauczyciele w systemie organizacji polskiego szkolnictwa sobotniego w Wielkiej Brytanii po drugiej wojnie światowej
Authors: Zamecka-Zalas Olga 
Published: March 2022

Edukacja ojczysta dziecka oraz przygotowanie w tym celu kadr pedagogicznych na uchodźstwie były nadrzędnym zadaniem polskich środowisk oświatowych przebywających po drugiej wojnie światowej w Wielkiej Brytanii. Gdy wycofane zostało uznanie dla rządu polskiego w Londynie, to właśnie działacze nauczycielscy przejęli ogromny trud utrzymania polskiego szkolnictwa, a tym samym przeciwdziałania wynarodowieniu młodego pokolenia Polaków. Celem artykułu jest ukazanie sytuacji nauczycieli w systemie organizacyjnym polskiego szkolnictwa oraz roli Zrzeszenia Nauczycielstwa Polskiego Zagranicą, które stworzyło podwaliny pod system szkół przedmiotów ojczystych. W artykule ponadto ukazano cele działalności nauczycieli, którzy byli ogarnięci wielką społeczną pasją i umiłowaniem tradycji, różnorodne formy kształcenia oraz ich ogromną rolę w wychowaniu dzieci w polskiej kulturze i tradycji.
W celu realizacji problemu badawczego zastosowano metodę monograficzną, krytykę analizy dokumentów i piśmiennictwa w tym zakresie.
Fundamentos de la regulación del crowdfunding: a propósito del Decreto de Urgencia 013-2020 que establece el Régimen Jurídico del Financiamiento Participativo Financiero
Authors: Jiménez Alemán José Alonzo 
Published: March 2022

El mercado de financiamiento es uno de los ejes de la promoción del emprendimiento, innovación y la creación de empleo. En ese orden de ideas, el financiamiento participativo (o también denominado crowdfunding) se posiciona como una herramienta de crédito importante que, entre otras características, a través de la innovación tecnológica, crea un ecosistema flexible de intermediación directa que permite conectar de forma masiva a inversores y promotores. A su vez, asegura una competencia efectiva de fuentes de financiamiento en el mercado de créditos.En el presente artículo, el autor expone un marco general sobre el crowdfunding y analiza críticamente su regulación en el régimen jurídico peruano. En esa misma línea, tomando en consideración la experiencia comparada, propone algunas recomendaciones normativas, a fin de potenciar el crecimiento de la figura y garantizar su adecuado funcionamiento en el mercado.
Effect of Planting Season on the Residue of Organophosphate in Chili (Capsicum annuum L.)
Authors: . Megawati , Sulaiman Muhammad Ikhsan , Zakaria Sabaruddin 
Published: March 2022

Background: Pesticide residues harm human health and are associated with hemopoietic disorders and many organ cancers. This study analyzes the pesticide residue content in chili cultivated by farmers in rainy and dry seasons, especially for organophosphates pesticides. The residual difference in chili was affected by planting seasons caused by farmers’ adoption of crop cultivation technologies, including pesticide application. Methods: The analysis was carried out using gas chromatography on 26 samples from 13 chili cultivation locations in Aceh province, Indonesia. Sixteen samples were harvested during the rainy season and the remaining ten were harvested during the dry season. Result: The results showed that pesticide residues were found in two red chili samples planted in the rainy season, while no residue was found from the dry season. In the rainy season’s harvested samples, the Organophosphate pesticide residues were chlorpyrifos 0.52 mg kg-1 and 0.16 mg kg-1 at Mutiara Tiga sub-district and Meurebo sub-district, respectively. From Meurebo sub-district profenofos 0.74 mg kg-1 was found. All detected pesticide residues samples are still below the maximum residue limit (MRL) set by the Government of Indonesia and CODEX Alimentarius.
Forecasting Rice Paddy Production in Aceh Using ARIMA and Exponential Smoothing Models
Authors: Nurviana Nurviana , Amelia Amelia , Riezky Purnama Sari Riezky Purnama Sari , Ulya Nabilla Ulya Nabilla , Talib Taufan 
Published: March 2022

Indonesia targets Aceh to be one of the paddy production centers and be able to carry out self-sufficient production in paddy and become a national granary. However, in reality, Aceh's paddy production in its province is not consistent from year to year. This province has not been able to meet the food needs of paddy independently, so that it supplies paddy from other regions due to the difficulty of detecting the presence of a surplus of paddy. The purpose of this research is to estimate the results of paddy production in Aceh for the future. The mathematical model that can be used is a time series model namely Autoregressive Integrated Moving Average (ARIMA) and Exponential Smoothing. The results of the estimation of paddy production in the next 5 years using the ARIMA (1,1,3) model are 2000616; 2017549; 1903585; 1944499; and 1929810. While the estimation results using the Winter Exponential Smoothing model are 1625925; 1645196; 1687667; 1605530; and 1555213. ARIMA model (1,1,3) produces an MSE/MAD value of , while the Winter Exponential Smoothing model produces an MSE/MAD value of . Therefore, it can be concluded that the Winter Exponential Smoothing model.