STORAGE EFFECT ON THE GC-MS PROFILING AND ANTIOXIDANT ACTIVITIES OF ESSENTIAL OILS FROM LEAVES OF ANNONA SQUAMOSA L.
Authors: Hammoud Sara , Jaber Ali , Ibrahim Ghassan , Cheble Edmomd 
Published: July 2022

Aim and objective: Medicinal plants, their biological activities, and their phytochemical contents are important for finding safe and potent new compounds for therapeutic use. In order to investigate the chemical contents and to evaluate the storage effect on the antioxidant activity of Lebanese Annona squamosa (AS) leaf essential oil, the current study was undertaken.
Methods: Shade-dried leaves of AS were taken from Batroun (Lebanon), and the essential oil (EO) was extracted by hydrodistillation. The gas chromatography-mass spectrometry (GC-MS) technique was used to analyze the composition of the EO. Concerning the antioxidant activity, two different methods namely radical scavenging activity (DPPH test) and ferric reducing antioxidant power (FRAP) were used.
Results: A total of 21 compounds were identified. The majority of the identified compounds belong to sesquiterpenoids. β-elemene (11.39 -14.14 %) and β-carophyllene (10.15 – 15.56 %) were the most abundant components. On the other hand, the storage of the plant materials containing the EOs or the EOs themselves leads to a loss in the volatile compounds, which is reflected in the bioactivity as shown in the results of the antioxidants assays. The EOs demonstrated antioxidant activities with IC50 lower than 9 μg.mL-1. DPPH test and FRAP test exhibited a strong positive correlation (r=0.99).
Conclusion: The obtained results suggest that EO extracts from AS have an antioxidant to protect people. Thus, the EO of fresh samples of AS can have interesting applications in versatile areas such as the pharmaceutical and food industries.

Peer Review History:
Received: 27 April 2022; Revised: 11 June; Accepted: 29 June, Available online: 15 July 2022
Academic Editor: Dr. Marwa A. A. Fayed, University of Sadat City, Egypt, maafayed@gmail.com
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Received file: Reviewer's Comments:
Average Peer review marks at initial stage: 6.0/10
Average Peer review marks at publication stage: 7.5/10
Reviewers:
Dr. Marwa A. A. Fayed, University of Sadat City, Egypt, maafayed@gmail.com
Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my
Dr. Nazim Hussain, North East Frontier Technical University, Arunachal pradesh, India, nhussain116@gmail.com
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