bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiphase Flowmeter Health Monitoring Strategy: Maximizing the Value of Real-Time Sensors and Automation for Industrial Revolution 4.0
Authors: Al-Kadem Mohammad , Gomaa Mohammed , Al Yateem Karam , Al Maghlouth Abdulmonam 
Published: April 2022

Summary
Metering with multiphase flowmeters (MPFMs) is a well-proven intelligent field technology, which utilizes complex flow models to accurately measure multiphase flow (oil, gas, and water) on a real-time basis. The major realized benefits of utilizing MPFM real-time sensor data technology are reduced maintenance costs, optimized maintenance schedules, and improved safety via minimizing visits and predicting well performance.
MPFMs also have the capability to perform comprehensive real-time test validation in a short period of time, making them a valuable tool. Leveraging their capabilities, engineers can perform various production analysis scenarios remotely using continuous data streaming to reduce and ultimately eliminate reliance on operators and vendors in remote fields. Nevertheless, such MPFMs require frequent maintenance, which could be quite difficult to manage when having a large number of meters.
An in-house application was developed to support those objectives and to remotely monitor the health of all MPFMs on a real-time basis. The in-house analytics system comprises four modules: Field Overview, MPFM Overview, Well Test Management, and Alarm Management. The steps used to avail this kind of solution cover infrastructure requirements, visualization requirements, optimization, and prediction. The solution was designed as the major interface for engineers to proactively detect and prevent major MPFM component failures and highlight all types of projected MPFM component failures. It proactively harvests preset alarms, which leads to preventing internal MPFM parts from failing. These alarms are generated based on critical parameters measured by the MPFM such as rates, differential pressure (DP), and radioactive measurements. Annual savings are realized through use of the monitoring solution by minimizing costly offshore visits and optimizing the visits based on problematic MPFMs.
The authors will discuss a newly developed MPFM health monitoring strategy based on real-time solutions, driving migration from a preventative to a predictive maintenance concept to reduce costs and optimize manpower time by eliminating unnecessary visits.
Bioinformatic Analysis of SNVS of the SPP1 Gene and the Role of Testicular Disease in Systemic Lupus Erythematosus
Authors: Rivera-Cameras A. , Ruiz-Ramírez A. V. , Gallegos-Arreola M. P , Morán-Moguel C. , Salazar-Paramo M. , Figuera L. E. , Dávalos-Rodríguez I.P 
Published: April 2022

Abstract
BACKGROUND: Secreted Phosphoprotein 1 (SPP1) is known to play an important role in inflammation and immunity. The differences in the presentation of systemic lupus erythematosus according to sex have been widely discussed such as the more severe and rapid course of the disease in men. We analyze gene expression in tissue-specific and variant-disease association of rs9138 and rs1126616 of SPP1 gene, we got the intersection proteins that are acting in both the protein-protein pathways of testicular disease and systemic lupus erythematosus. A tissue-specific gene expression analysis was performed in the Genotype-Tissue Expression (GTEx v8), ENSEMBL and EBI-EMBL databases; focusing on tissues that could be related to inflammation processes or cells involved in the production of INF. A variant-disease association was made in DisGenet platform. Protein-protein interaction network related to testicular disease and systemic lupus erythematosus was performed in Cytoscape.RESULTS: Tissue-specific gene expression showed a main influence on the expression in testis tissue. In the variant-disease association, rs1126616 was associated with systemic lupus erythematosus and rs9138 with autoimmune disease.17 proteins were found involved in the development of testicular disease and systemic lupus erythematosus. The pathways in which are intervening are positive regulation of cytokine production, regulation of interferon-gamma production, negative regulation of interferon-gamma production, positive regulation of interferon-gamma production, regulation of immune system process. CONCLUSIONS: The positive association between testicular function and the development of systemic lupus erythematosus has been seen previously, particularly when there is testicular hypofunction. The key role of the rs9138 variant in activating high levels of Interferon-α and secreted phosphoprotein 1 has been reported, but the role of the rs1126616 variant has not been proposed before. It suggests that men with systemic lupus erythematosus have a higher risk of comorbidity, this may justify its consideration in the treatment of patients, this information opens avenues for future research focused on different tissues.
The R2R3 MYB transcription factors MaMYBF and MaMYB1 regulate flavonoid biosynthesis in grape hyacinth
Authors: Wang Jiangyu , Zhang Han , Tian Shuting , Hao Wenhui , Chen Kaili , Du Lingjuan 
Published: April 2022

Abstract
R2R3 MYBs play vital roles in the regulation of flavonoid biosynthesis. However, the regulatory network of R2R3 MYBs in flavonoid biosynthesis is not fully understood in grape hyacinth (Muscari spp.). Here, we identified two R2R3 MYBs, MaMYBF and MaMYB1, as potential regulators of flavonol and anthocyanin biosynthesis, respectively. MaMYBF and MaMYB1 expression was elevated during flower development and was light-induced, and the expression patterns were correlated with those of the flavonoid structural genes MaFLS and MaDFR, respectively. The BiFC assay confirmed that MaMYB1 interacts with MabHLH1, but MaMYBF does not. A dual luciferase assay revealed that MaMYBF alone strongly activated pMaFLS, and its activation was attenuated at reduced doses of MaMYBF in the presence of MabHLH1, MaMybA, and MaMYB1. MaDFR transcription mediated by MaMybA and MabHLH1 was inhibited by MaMYB1. Moreover, overexpression of MaMYBF and MaMYB1 in tobacco reduced flower pigmentation and repressed the expression of flavonoid pathway key structure genes. Therefore, MaMYBF regulates the flavonol pathway independently of cofactors, whereas MaMYB1 regulates anthocyanin biosynthesis by binding to MabHLH1 and disrupting the MaMybA-bHLH complex in grape hyacinth. Our results provide new insights into the intricate regulatory network of flavonoids in grape hyacinth involving the regulation of both flavonol and anthocyanin.
The Impact of Severe Infectious Pulmonary Exacerbation on fat-free mass in Hospitalized Adults with Cystic Fibrosis
Authors: Silveira Michelle Nunes , Jacobsen Letícia Bauer , Fangueiro Fabiana Salatino , Ziegler Bruna , Dalcin Paulo de Tarso Roth 
Published: April 2022

Abstract
Cystic fibrosis (CF) patients experience a continuous acute phase of inflammatory and catabolic state. Longitudinal study to assess the impact of severe infectious pulmonary exacerbation on fat-free mass (FFM) and on the fat-free mass index (FFMI) in adults with CF who required hospital admission. Nutritional assessment using anthropometry and bioimpedance, Visual Appetite Scale (VAS), Kanga Score, functional assessment by spirometry and a 6-minute walk test. Inflammatory biochemical markers cortisol and C-Reactive Protein were also used. Results: The multivariate linear regression analysis identified only sex as an independent variable significantly associated with improvement in the FFMI (B= -1.81, 95% CI= -3.10; -0.51, p=0.008). As for the increase in FFM, two significantly associated independent variables were identified: sex (B= -10.38, confidence interval – 95% CI= -14.70; -6.06, p<0.001); and forced vital capacity (FVC) % of predicted (B=0.13, 95% CI=0.04; 0.23, p=0.006). There was a significant improvement in FFM and FFMI between the start of hospitalization and the 14th day. Gender and FVC % of predicted were responsible for predicting FFM improvement. For the FFMI, the predictive variable of this improvement was gender only, with males having the greatest increase in FFM and FFMI.
Upregulation of long noncoding RNA MALAT1 in colorectal cancer promotes radioresistance and malignance
Authors: Shen Wenqi , Yu Qifeng , Pu Yuwei , Xing Chungen 
Published: April 2022

Abstract
Background: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a conserved transcript with 8,000 nt, is highly associated with malignancy of numerous cancer types. However, the function of MALAT1 plays in regulating the response to radiotherapy in colorectal cancer (CRC) remains unclear. Thus, the object of this study is to investigate the functions of MALAT1 in CRC radioresistance.Methods: First, the expression of MALAT1 in colon adenocarcinoma (COAD) was analyzed through the Cancer Genome Atlas (TCGA) database. Then, we detected the expression level of MALAT1 in tumor tissues and CRC cell lines and analyzed the relevance of MALAT1 and clinicopathological parameters. In the end, the effect of silencing MALAT1 on the radiosensitivity of CRC cells was investigated, and its potential mechanism was preliminarily illustrated.Results: The analysis of TCGA data showed that MALAT1 was closely related to the type of tumor, and high expression of MALAT1 was remarkably relevant to poor outcome. MALAT1 was highly expressed in CRC tissues and cell lines and related to tumor stages. Knockdown of MALAT1 could significantly suppress colony survival, proliferation, and migration and increase apoptosis, G2/M phase arrest, and formation of gamma-H2AX foci in HCT116, whether in combination with X-rays or not. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstated that the regulated proteins were principally enriched in the glycosaminoglycan degradation pathway after silencing MALAT1.Conclusion: Our results implied that MALAT1 was highly expressed in CRC and associated with tumor stage and prognosis. Silencing MALAT1 can increase HCT116 cell radiosensitivity, which may be potentially influenced by glycosaminoglycan degradation pathway.
The burden of antibiotic resistance bacterial isolates from chickens, goats, cattle and pigs in Bvumbwe, Malawi
Authors: Kalumbi Martin H , Katuah Zefaniah J , Katiniche Blessings , Chinyama Robert , Thugo Simon , Nyirenda Atusaye E , Moyo Donita , Kamwendo Chikondi , Chagwa Patrick , Mlozen Madalitso , Bonya Elias , Nyanda Adam M , Majamanda Jonathan , Taika Wilfred , Linje Linly 
Published: April 2022

Abstract
Background: Antibiotic resistance is a serious problem worldwide affecting human and animal health. Some bacteria are resistant to more than one antibiotic and this multidrug-resistance is one of the top 10 threats to global health. There is a potential that antibiotic resistant strains are passed on from animals to humans leading to more serious bacterial infections and burden. The study was therefore conducted to determine antibiotic resistant bacterial profile in chickens, cattle, goats and pigs.Methods: Laboratory microbiological experiments were conducted on droppings, mouth, nose and hooves samples obtained from chickens, cattle, goats and pigs from Bvumbwe, Malawi. Gram stain was performed to identify the organisms and biochemical reactions were used to confirm bacterial species. The susceptibility of the bacterial isolates to commonly used antibiotics in Malawi was done using disk diffusion method. Results: Bacteria were detected in all the 110 (100%) samples. Citrobacter, S. aureus, Bacillus, E. coli, Clostridium, Klebsiella, Streptococcus, other coliforms and Staphylococcus species were isolated. Among the samples, Bacillus species were the most isolated (35%) and distributed (41.8%) organisms while Streptococcus species were the least isolated (1%) and distributed (3%) organisms. S. aureus, Streprococcus and Citrobactor species demonstrated resistance to at least four antibiotics including Gentamycin, Tetracycline, Ampicillin, Ciprofloxacin, Chloramphenicol and Erythromycin. Highest resistance of 41.7 % was observed in S. aureus followed by Citrobacter species of 33.3%. Conclusion: Healthy farm animals such as chickens, cattle, goats and pigs harbour antibiotic resistance strains. High levels of Ampicillin and Tetracycline resistance in bacterial strains are common in Bvumbwe, Malawi. Efforts are needed to control the use, distribution, storage and sale of antibiotics in veterinaries.
Thoracic endovascular aortic repair of blunt traumatic thoracic aorta injury: A propensity score-matched analysis of the long-term clinical outcome
Authors: Kan Yuanqing , Xie Xinsheng , Yuan Ye , Guo Daqiao , Si Yi , Zhang Wan , Shi Zhenyu , Fu Weiguo , Wang Lixin 
Published: April 2022

Abstract
Objectives: Patients of blunt traumatic thoracic aortic injury (BTAI) have different vessel property with those of primary type B aortic dissection (pATBAD). We hypothesize that thoracic endovascular aortic repair (TEVAR) may have better efficacy for BTAI patients than for pATBAD. This study aimed to compare the outcome of TEVAR for these two populations based on a prospectively built clinical database. Methods: To reduce the baseline characteristic disparity between two groups, propensity score analysis with a match of 1:2 ratio was employed in BTAI and pATBAD patients who underwent TEVAR in a tertiary center from 2009 to 2018. Data including demographic, perioperative and follow up results were retrieved and analysed.Results: A total of 41 BTAI and 82 pATBAD patients were enrolled. Baseline characteristics were comparable between both groups after match. The pATBAD group had a remarkably higher incidence of hypertension (p < 0.001). The total stent-graft covered length was significantly longer in the pATBAD group than in the BTAI group (p = 0.016). Aortic-related reintervention rate was significantly higher in the pATBAD group than in the BTAI group (p = 0.027). Higher rate of abdominal complete FL thrombosis was achieved in BTAI group than in pATBAD group (69.2% vs. 28.6%, p = 0.001).Overall, the early outcomes and long-term survival were similar in both groups. Conclusions: TEVAR for BTAI patients show the acceptable clinical efficacy and safety when compared with the pATBAD. This study could provide more solid evidence for current clinical practice to support TEVAR for BTAI.
First Line Anti-Retroviral (ARV) Drugs Disrupt Follicular Development in Female Wistar Rats
Authors: Ohihoin Aigbe Gregory , Ohihoin Esther Ngozi , Ujomu Ifeoma , Bakare Airat , Olarenwaju Oladeji , Okafor Arinze , Ojetunde Mercy Mayowa , Ayoola Joy Boluwatife , Aina Oluwagbemiga , Ajibaye Sola , Taylor-Robinson Simon D. 
Published: April 2022

Abstract
INTRODUCTION: There are genuine concerns that long-term use of antiretroviral drugs may be associated with reproductive complications in females. This study aimed to ascertain the effect of highly active antiretroviral drugs on the ovarian reserve and reproductive potential of female Wistar rats and by extension to HIV-positive human females.METHODS: A total of 25 female Wistar rats, weighing between 140g-162g, were randomly allotted into non-intervention and intervention groups, receiving the anti-retroviral drugs, Efavirenz (EFV), Tenofovir Disoproxil Fumarate (TDF), Lamivudine (3TC), and a fixed-dose combination (FDC). The dosage was administered orally at 8 am daily for 4 weeks. Serum concentrations of anti-Mullerian hormone (AMH), follicle stimulating hormone (FSH), luteinising hormone (LH) and estradiol were measured using standard biochemical techniques (ELISA). Follicular counts were made on fixed ovarian tissue from the sacrificed rats.RESULTS: The mean AMH level for the control group and the EFV, TDF, 3TC, and the FDC groups were 11.20, 6.75, 7.30, 8.27, and 6.60 pmol/L, respectively. The EFV and FDC groups had the lowest AMH, compared to the other groups, but there was no statistically significant difference in AMH across the groups. The mean count of antral follicles was significantly lower in the group that received EFV when compared to the other groups. The corpus luteal count was significantly higher in the control group than in the intervention groups (p=0.033). CONCLUSION: The study demonstrated a disruption in the reproductive hormones of female Wistar rats receiving antiretroviral regimens containing EFV. Clinical studies are required to determine if these changes are seen in women receiving EFV-based treatment, as this may compromise reproductive function and predispose them to early menopause.
Assessment of the relationships between Ki67 expression and neoadjuvant treatment response and prognosis in breast cancer using two types of response evaluation systems
Authors: Sullu Yurdanur , Demirag Guzin , Kuru Bekir , Ozen Necati , Karagoz Filiz 
Published: April 2022

Abstract
Background: Although it is not a good prognostic marker for all breast cancers, pathological complete response (pCR) is an endpoint in evaluating neoadjuvant chemotherapy (NAC) efficacy. Ki67, a proliferation marker, has a complex role as a predictive marker in determining the NAC response. This study aimed to investigate the relationship between pretreatment and posttreatment Ki67 levels, posttreatment Ki67 change and treatment response using the Miller–Payne (MP) and Residual Cancer Burden (RCB) response assessment systems.Methods: A total of 178 invasive breast carcinoma patients who underwent NAC were included in the study. Ki67 levels were evaluated by immunohistochemical method in trucut biopsy and surgical excision specimens. Treatment response in the surgical excision specimen was classified according to both MP and RCB classifications. We investigated the relationships between pretreatment Ki67 level, posttreatment Ki67 level and posttreatment change in Ki67 with NAC response and survival. Additionally, the cut-off value of the pretreatment Ki67 level for pCR and nonpathological response (pNR) was investigated.Results. The pretreatment Ki67 level was significantly higher in the pCR group than in the partial response (pPR) and pNR groups (p<0.001) in both the MP and RCB systems. The posttreatment Ki67 level was significantly higher in the pNR group than in the pPR group (p<0.001) in both systems. There was a negative correlation between pretreatment Ki67 and disease-free survival (DFS) in the luminal B HER2-negative subtype (r=-0.377, p=0.036) and a significant negative correlation between posttreatment Ki67 and overall survival (OS) in the HER2-positive subtype (r=-0.544, p= 0.0209). A significant correlation was found between the posttreatment Ki67 change and the degree of response in the luminal B HER2-positive and HER2-positive subtypes (p<0.05). The Ki67 cut-off value was 37.5 for pCR in both the MP and RCB classification systems (95% CI 0.673-0.833 and 95% CI 0.66-0.827) (p<0.001). There was a significant moderate agreement between the MP and RCB systems (p<0.001).Conclusions: Pre- and posttreatment Ki67 levels may be used to assess treatment response in various molecular subtypes of breast cancer. In this study, the cut-off value of Ki67 for pCR was 37%. More aggressive treatments may be considered in luminal B HER2-negative tumours with high pretreatment Ki67 levels and tumours that do not show a Ki67 decrease.
Comparative effect of cooling on the physico-chemical-sensory properties of ghee from cow and buffalo milk, and evaluation of the low-fat spread prepared from cow and buffalo milk ghee
Authors: Deverao Patange Dnyaneshwar , Vinayak Dhanake Virshasen , Chandran Deepak , Radha Radha , Kumar Manoj , Lorenzo Jose Manuel 
Published: April 2022

Abstract
Low-fat spread (LFS) is the product harmonizing with the idea of healthy nutrition. At the same time, it has a good taste and flavour, as well as very good spreadability at refrigerator temperature. The present investigation studied the effect of method of cooling on the properties of cow and buffalo milk ghee, and comparative evaluation of LFS prepared from them. Slowly pre-cooled cow ghee had intense yellow colour than rapidly pre-cooled cow ghee, whereas slowly pre-cooled buffalo ghee has creamish white colour and rapidly pre-cooled buffalo ghee had white colour. Rapidly pre-cooled cow ghee had a very smooth and pasty texture than rapidly pre-cooled buffalo ghee. The LFS of cow ghee had shown maximum sensory scores for colour and appearance, body and texture, spreadability, and overall acceptability, as compared to buffalo ghee LFS. Chemically it was observed that both the LFS differs in FFA content, while they had similar fat, protein, carbohydrate, ash and total solids content, as well as pH. Oiling off and wheying off was found higher in cow ghee LFS over buffalo ghee LFS. Colour, appearance and flavour score were found improved by the addition of butter annatto colour and diacetyl flavour respectively. Color and appearance, body and texture, spreadability, as well as overall acceptability scores were higher for cow ghee LFS when subjected to 35°C for 10, 20, and 30 minutes. It was found that after 10 minutes of exposure to 35°C, the physical qualities of both LFS were unchanged, but the sensory properties diminished as time passed.
Response to immune checkpoint inhibitor combined therapies in metastatic BRAF mutant lung cancers
Authors: Yan Ningning , Zhang Ziheng , Guo Sanxing , Huang Siyuan , Shen Shujing , Xu Liang , Zhang Huixian , Li Xing 
Published: April 2022

Abstract
The efficacy of immune checkpoint inhibitors (ICIs) combination regimens in BRAF mutant non-small cell lung cancers (NSCLCs) remains poorly explored. Immunotypes, including programmed death-ligand 1 (PD-L1) levels and tumor mutation burden (TMB), were evaluated. Objective response rate (ORR), disease control rate, progression-free survival (PFS), and overall survival (OS) of patients treated with ICI combination therapies were analyzed. A total of 150 patients conducted PD-L1 tests, wherein PD-L1 expression of 22 patients was greater than or equal to 50% (14.7%). Among the 38 TMB-evaluated patients, 28 had fewer than 10 mutations per megabase (73.7%). For patients treated with ICI combined regimens, median PFS was 6 months and median OS was 21.9 months. When stratified by treatment line, OS presented a significant difference (p=0.01), while PFS did not (p=0.40). The ORR for the treated patients was 36.7%. When stratified by BRAF mutation type, no differences were observed in PFS (p=0.74) and OS (p=0.94). Furthermore, no difference was found in PD-L1 expression (p = 0.06) or TMB (p=0.22) between the responders and non-responders. BRAF-mutant NSCLCs is associated with low levels of PD-L1 expression and low/intermediate TMB. ICIs combined therapies present promising activity and could provide an option for BRAF-mutated NSCLCs.