bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mycobiota profile, phenology, and potential toxicogenic and pathogenic species associated with stored groundnuts (Arachis hypogaea L.) from the Volta Region, Ghana
Authors: Kortei Nii Korley , Tetteh Rachel Adinorkie , Wiafe‐Kwagyan Michael , Amon Denick Nii Kotey , Odamtten George Tawia 
Published: January 2022

AbstractThis study updates the mycobiota resident in groundnut seeds, their phenology during storage with the view to ascertain their occurrence, potential toxigenic species, and pathologically important species in the stored samples. The moisture content of the seeds ranged from 5.7% to 6.5% within the stipulated safe moisture content of 8% for extension of shelf life. Culturing the seeds on mycological media (Sabouraud's Dextrose Agar SDA; Oxytetracycline Glucose Yeast Extract OGYE, Potato Dextrose Agar, PDA) caused a de novo growth of the quiescent spores at 28–30°C for 7–14 days. Fungal population counts on the three media ranged from 2.01 to 2.16 log10 CFU/g samples to a final 6‐month count of 1.67–2.60 log10 CFU/g. Eighteen different fungal species belonging to ten genera were encountered on the media, namely Aspergillus, Cladosporium, Curvularia, Fusarium, Penicillium, Trichoderma, Rhizopus, Rhodotorula, Sporendonema, and Paecilomyces. Aspergillus spp. (A. niger, A. flavus, A. fumigatus, and A. terreus) were the most frequently isolated, followed by Fusarium species (F. oxysporum, F. solani, and F. verticillioides), Trichoderma (T. harzianum and T. viride), Rhizopus spp (R. oligosporus and R. stolonifer), and Penicillium verrucosum. The species which were seed borne (A. niger, A. flavus, A. terreus, A. fumigatus, F. solani, F. verticillioides, T. viride, C. herbarum, and Curvularia lunata) were isolated on both surface sterilized and non‐surface sterilized seeds. The phenology of the encountered fungal species generally followed five patterns. The most frequently isolated Aspergillus niger, A. flavus, and A. fumigatus predominated throughout the 6 months sampling period, while A. ustus and A. terreus appeared sporadically and disappeared. The early colonizers (R. oligosporus, R. stolonifer, and Paecilomyces) could not be isolated after 2–3 months owing presumably to stronger antibiosis competition from the Aspergillus species. The most predominant Aspergillus species initially constituted 36%–48% of the total population but declined to 10%–36% in 6 months. Mycobiota encountered with mycotoxigenic potential and human health importance were A. niger, A. flavus, A. fumigatus, F. verticillioides, and Penicillium verrucosum. Other species of pathological importance to plants were Curvularia lunata and Fusarium oxysporum. The practical implications of these findings are discussed.
Fate of aflatoxins M1 and B1 within the period of production and storage of Tarkhineh: A traditional Persian fermented food
Authors: Moradi Leila , Paimard Giti , Sadeghi Ehsan , Rouhi Milad , Mohammadi Reza , Noroozi Razieh , Safajoo Saeede 
Published: January 2022

AbstractThe objective of the study was to assess the amount of aflatoxin M1 (AFM1) and aflatoxin B1 (AFB1) during fermentation, drying, and storage of Tarkhineh—a traditional Persian fermented food—over four months. Tarkhineh samples were produced based on a traditional method. Various concentrations of AFB1 (2.5, 5, 7.5, and 10 µg/kg) and AFM1, stood at 0.25, 0.5, 0.75, and 1 µg/kg, were added to Iranian yogurt drink, called doogh, samples. Tarkhineh samples were evaluated for AFB1 and AFM1 on days 0, 2, 6, and 8 and also after drying and four months of storage. In cases of repeatability, recovery, and reproducibility, the high‐performance liquid chromatography through fluorescence detector (HPLC‐FD) method was successfully done to demonstrate aflatoxins (AFs) in Tarkhineh samples. The fermentation process had a considerable consequence on the reduction in AFM1 and AFB1 as compared to the control group, evidenced by 65.10%–81.20% and 55.80%–74.10%, respectively, after eight days of fermentation (p < .05). The highest reduction in AFB1 existed in samples containing 2.5 µg/kg toxin, followed by 5, 7.5, and 10 µg/kg, respectively. A similar trend was found for AFM1, as the highest concentration was found in samples containing 0.25 µg/kg toxin, followed by 0.5, 0.75, and 1 µg/kg, respectively.
Contagion or flight‐to‐quality? The linkage between oil price and the US dollar based on the local Gaussian approach
Authors: Ming Lei , Shen Yao , Yang Shenggang , Dong Minyi 
Published: January 2022

AbstractThe paper utilizes the multilateral prices of West Texas Intermediate futures and the US dollar to study the nature of oil‐dollar linkages. The empirical findings confirm a contagion effect between these two markets in the 1990 Gulf War, while flight‐to‐quality phenomena from oil to the US dollar are confirmed as a common feature for the 2008 and 2014 oil crashes, implicitly emphasizing the safe‐haven role of the US dollar during oil market turmoil. These results offer significant evidence for nonlinear and asymmetric relationship between the crude oil market and the US dollar market during all periods of oil crashes.
Microwave ablation versus laparoscopic resection as first‐line therapy for solitary 3–5‐cm HCC
Authors: Wang Zhen , Liu Miao , Zhang De‐zhi , Wu Song‐song , Hong Zhi‐xian , He Guang‐bin , Yang Hong , Xiang Bang‐de , Li Xiao , Jiang Tian‐an , Li Kai , Tang Zhe , Huang Fei , Lu Man , Chen Ji‐an , Lin Yu‐cheng , Lu Xiao , Wu Yu‐quan , Zhang Xiao‐wu , Zhang Ye‐fan , Cheng Chao , Ye Huo‐lin , Wang Lan‐tian , Zhong Hua‐ge , Zhong Jian‐hong , Wang Lu , Chen Miao , Liang Fang‐fang , Chen Yi , Xu Yan‐song , Yu Xiao‐ling , Cheng Zhi‐gang , Liu Fang‐yi , Han Zhi‐yu , Tang Wei‐zhong , Yu Jie , Liang Ping 
Published: January 2022

Abstract

Background and Aims
The study objective was to compare the effectiveness of microwave ablation (MWA) and laparoscopic liver resection (LLR) on solitary 3–5‐cm HCC over time.

Approach and Results
From 2008 to 2019, 1289 patients from 12 hospitals were enrolled in this retrospective study. Diagnosis of all lesions were based on histopathology. Propensity score matching was used to balance all baseline variables between the two groups in 2008–2019 (n = 335 in each group) and 2014–2019 (n = 257 in each group) cohorts, respectively. For cohort 2008–2019, during a median follow‐up of 35.8 months, there were no differences in overall survival (OS) between MWA and LLR (HR: 0.88, 95% CI 0.65–1.19, p = 0.420), and MWA was inferior to LLR regarding disease‐free survival (DFS) (HR 1.36, 95% CI 1.05–1.75, p = 0.017). For cohort 2014–2019, there was comparable OS (HR 0.85, 95% CI 0.56–1.30, p = 0.460) and approached statistical significance for DFS (HR 1.33, 95% CI 0.98–1.82, p = 0.071) between MWA and LLR. Subgroup analyses showed comparable OS in 3.1–4.0‐cm HCCs (HR 0.88, 95% CI 0.53–1.47, p = 0.630) and 4.1–5.0‐cm HCCs (HR 0.77, 95% CI 0.37–1.60, p = 0.483) between two modalities. For both cohorts, MWA shared comparable major complications (both p > 0.05), shorter hospitalization, and lower cost to LLR (all p < 0.001).

Conclusions
MWA might be a first‐line alternative to LLR for solitary 3–5‐cm HCC in selected patients with technical advances, especially for patients unsuitable for LLR.
Impact of anthropogenic disturbances on carbon cycle changes in terrestrial‐aquatic‐estuarine continuum by using an advanced process‐based model
Authors: Nakayama Tadanobu 
Published: January 2022

AbstractRecent research has shown that inland water—including rivers, lakes, and groundwater—may play some role in carbon cycling, although the extent of its contribution has remained uncertain due to the limited amount of reliable data available. To evaluate global changes in the carbon cycle due to anthropogenic factors, such as application of fertilizer and manure, in major rivers including 130 tidal estuaries over an 18‐year period (1998–2015), the present study used an advanced process‐based model derived by coupling a process‐based national integrated catchment‐based eco‐hydrology (NICE) model with various biogeochemical cycle models (NICE‐BGC). Generally, total nitrogen and phosphorus transports in overland flow were found to increase. In contrast, total suspended sediment in overland flow decreased in some regions because the vegetation was able to expand to cover the ground, resulting in less erosion. NICE‐BGC simulated the difference in carbon budget in major rivers with and without nutrient application. Generally, CO2 degassing across the water‐air interface decreased and particulate organic carbon (POC) increased in most rivers through variations in carbon budget, where excess nutrients might stimulate gross primary production of carbon‐rich algal biomass. The simulated result also showed that the estuarine carbon cycle was sensitive to intense anthropogenic disturbances reflected by nutrient load, seawater temperature, increases in sea level, and ocean acidification. Extension of previous studies only by categorizing MARgins and CATchments Segmentation (MARCATS) segment numbers showed that the estimated total CO2 flux from the world's estuaries was 0.14 Pg C/year. Generally, the simulation showed that incorporation of the nutrient cycle into the terrestrial‐aquatic‐estuarine continuum improved estimates of net land flux and carbon budget in inland waters, thus emphasizing that the effect of estuarine inland water should be explicitly included in the global carbon model to minimize the range of uncertainty.
mitoDataclean: A machine learning approach for the accurate identification of cross‐contamination‐derived tumor mitochondrial DNA mutations
Authors: Su Liping , Guo Shanshan , Guo Wenjie , Ji Xiaoying , Liu Yang , Zhang Huanqin , Huang Qichao , Zhou Kaixiang , Guo Xu , Gu Xiwen , Xing Jinliang 
Published: January 2022

AbstractNext‐generation sequencing (NGS) of mitochondrial DNA (mtDNA) has widespread applications in aging and cancer studies. However, cross‐contamination of mtDNA constitutes a major concern. Previous methods for the detection of mtDNA contamination mainly focus on haplogroup‐level phylogeny, but neglect haplotype‐level differences, leading to limited sensitivity and accuracy. In our study, we present mitoDataclean, a random‐forest‐based machine learning package for accurate identification of cross‐contamination, evaluation of contamination levels and detection of contamination‐derived variants in mtDNA NGS data. Comprehensive optimization of mitoDataclean revealed that training simulation with mixtures of small haplogroup distance and low polymorphic difference was critical for optimal modeling. Compared to existing methods, mitoDataclean exhibited significantly improved sensitivity and accuracy for the detection of sample contamination in simulated data. In addition, mitoDataclean achieved area under the curve values of 0.91 and 0.97 for discerning genuine and contamination‐derived mtDNA variants in a simulated Western dataset and private sequencing contamination data, respectively, suggesting that this tool may be applicable for different populations and samples with different sources of contamination. Finally, mitoDataclean was further evaluated in several private and public datasets and showed a robust ability for contamination detection. Altogether, our study demonstrates that mitoDataclean may be used for accurate detection of contaminated samples and contamination‐derived variants in mtDNA NGS data.
Impact of two waves of Sars‐Cov2 outbreak on the number, clinical presentation, care trajectories and survival of patients newly referred for a colorectal cancer: A French multicentric cohort study from a large group of university hospitals
Authors: Kempf Emmanuelle , Priou Sonia , Lamé Guillaume , Daniel Christel , Bellamine Ali , Sommacale Daniele , Belkacemi Yazid , Bey Romain , Galula Gilles , Taright Namik , Tannier Xavier , Rance Bastien , Flicoteaux Rémi , Hemery François , Audureau Etienne , Chatellier Gilles , Tournigand Christophe ,   on behalf of the Assistance Publique—Hôpitaux de Paris Cancer Group
Published: January 2022

AbstractThe SARS‐Cov2 may have impaired care trajectories, patient overall survival (OS), tumor stage at initial presentation for new colorectal cancer (CRC) cases. This study aimed at assessing those indicators before and after the beginning of the pandemic in France. In this retrospective cohort study, we collected prospectively the clinical data of the 11.4 million of patients referred to the Greater Paris University Hospitals (AP‐HP). We identified new CRC cases between 1 January 2018 and 31 December 2020, and compared indicators for 2018‐2019 to 2020. pTNM tumor stage was extracted from postoperative pathology reports for localized colon cancer, and metastatic status was extracted from CT‐scan baseline text reports. Between 2018 and 2020, 3602 and 1083 new colon and rectal cancers were referred to the AP‐HP, respectively. The 1‐year OS rates reached 94%, 93% and 76% for new CRC patients undergoing a resection of the primary tumor, in 2018‐2019, in 2020 without any Sars‐Cov2 infection and in 2020 with a Sars‐Cov2 infection, respectively (HR 3.78, 95% CI 2.1‐7.1). For patients undergoing other kind of anticancer treatment, the percentages are 64%, 66% and 27% (HR 2.1, 95% CI 1.4‐3.3). Tumor stage at initial presentation, emergency level of primary tumor resection, delays between the first multidisciplinary meeting and the first anticancer treatment did not differ over time. The SARS‐Cov2 pandemic has been associated with less newly diagnosed CRC patients and worse 1‐year OS rates attributable to the infection itself rather than to its impact on hospital care delivery or tumor stage at initial presentation.
Introduction of Special Section of Infant Mental Health Journal: Meeting the needs of vulnerable infants and families during COVID‐19: Moving to a telehealth approach for home visiting implementation and research
Authors: Dozier Mary 
Published: January 2022

AbstractParents of infants and young children who experience harsh circumstances are among those most vulnerable to the added stressors associated with COVID‐19. Home visiting models have been shown to enhance outcomes for parents and infants when delivered in person, but in many parts of the world, the pandemic rendered in‐person home visits difficult or impossible. In this special section, we examine adaptations made by home visiting programs to allow continued service delivery through telehealth, and strategies for assessing whether interventions maintain reach and fidelity when implemented remotely. In the first paper, Bullinger et al. (program implementers of SafeCare) provide evidence of the increased risk of maltreatment during COVID‐19 for many families, and thus the need for home visiting services. Rybińska et al., developers and implementers of Family Connects, present evidence regarding their success in reaching families through telehealth. Roben and colleagues, in the third paper, report that clinicians implementing Attachment and Biobehavioral Catch‐up through telehealth maintained fidelity at similar rates seen through in‐person implementation. Finally, Tabachnick et al. describe procedures for collecting physiological data from infants and parents while conducting assessments remotely.
Morphology control in high‐efficiency all‐polymer solar cells
Authors: Zhou Kangkang , Xian Kaihu , Ye Long 
Published: January 2022

AbstractAll‐polymer solar cells (All‐PSCs) have attracted tremendous research interest in the recent decade due to the great potentials in stretchable electronic applications in terms of long‐term stability and mechanical stretchability. Driven by the molecular design of novel polymer acceptors and morphology optimization, the power conversion efficiency (PCE) of All‐PSCs has developed rapidly and now exceeded 17%. This review outlines the promising strategies for high‐performance All‐PSCs from the aspect of morphology control with the motivation to rationally guide the optimization. In this review, we briefly discuss the thermodynamic mixing principles of all‐polymer blends and the effects of the molecular structure of conjugated polymers on thin‐film morphology in All‐PSCs. The crucial role of molecular miscibility in influencing morphological features and performance metrics was highlighted. We also expound on the effective methods of controlling film morphology through properly tuning the aggregation behavior of polymers. In particular, insightful studies on the commonly used naphthalene diimide‐based acceptor polymers and the newly emerging polymerized nonfullerene small molecule acceptors (ITIC‐series, Y6 ‐series, etc) are discussed in detail. Finally, we present an outlook on the major challenges and the new opportunities of All‐PSCs for efficiency breakthroughs targeting 20%.
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Clinical values of expression signature of circCDR1AS and circHIAT1 in prostate cancer: Two circRNAs with regulatory function in androgen receptor (AR) and PI3K/AKT signaling pathways
Authors: Aghajani Mir Mahsa , Dinmohammadi Hossein , Moudi Emadoddin , Motamed Nima , Daraei Abdolreza 
Published: January 2022

AbstractBackgroundProstate cancer (PCa) is a genetically heterogeneous disease with highly molecular aberrations. It has been revealed that a newly discovered class of non‐coding RNAs called circular RNAs (circRNAs) play key roles in dictating tumor behaviors and phenotypes of the prostate tumors. In the current study, our aim was to determine the expression profiles of circHIAT1 and circCDR1AS in PCa compared with benign prostatic hyperplasia (BPH) tissues, as well as their clinicopathological relevance.MethodsThe 50 prostate tissues including 25 PCa tissues and 25 BPH samples were collected for analyzing the expression levels of target circRNAs by quantitative real‐time PCR (qRT‐PCR).ResultsThe results revealed that expression of circCDR1AS was significantly elevated in PCa compared with the BPH (p < 0.05). We also observed that PCa patients over the age of 60 had a higher expression of the circCDR1AS than patients under the age of 60 (p = 0.017). Moreover, a lower expression level of circHIAT1 was found in the PCa than BPH tissues (p < 0.05), and finally, the findings indicated that the area under the curve (AUC) of circCDR1AS was 0.848, with 92% sensitivity and 76% specificity, as well as an AUC of 0.828, with the 80% sensitivity and 76% specificity for circHIAT1.ConclusionThese observations suggest that the abnormal expression of circCDR1AS and circHIAT1 can be regarded as two different types of molecular pathology with potential biomarker values for PCa, although further studies are needed.
Morphological and porosity changes in primary enamel surface after an in vitro demineralization model
Authors: González‐Sotelo Arlete , Contreras‐Bulnes Rosalía , Rodríguez‐Vilchis Laura Emma , de los Angeles Moyaho‐Bernal María , Rubio‐Rosas Efraín , Teutle‐Coyotecatl Bernardo , Mézquita‐Rodrigo Irina 
Published: January 2022

AbstractIn vitro models are very useful in dentistry, especially to evaluate preventive methods against dental caries. Although they have been used for more than 30 years, specific demineralization models have not been established for primary enamel, which is more prone to demineralization than permanent enamel. This study evaluates porosity changes in primary enamel surface after a demineralization model through a scientifically validated analytical tool. Nine healthy human anterior primary teeth extracted for therapeutic reasons were included in this study, previous informed consent. The samples were randomly assigned to three groups n = 3: G1_2D, G2_4D, and G3_7D. Scanning electron microscopy (SEM) images at ×200 and ×1000 were taken during two stages: before demineralization (BD) and after demineralization (AD). Morphological characterization was observed at ×1000, while porosity (pore count and perimeter) was analyzed by the ImageJ program, using ×200 SEM images previously converted. Several statistical analyses were used to determine differences (p ≤ .05). Morphological characterization AD revealed new pits and cracks on the enamel surface in G1_2D and G2_4D groups. Localized eroded enamel areas were observed in G3_7D. Pore count of enamel surface BD ranged from 64.26 ± 37.62 to 97.93 ± 34.25 and AD ranged from 150.06 ± 64.86 to 256 ± 58.14. AD, G_4D exhibited a decrease in pore perimeter contrary to G_2D and G_7D. Significant differences were observed. Finally, morphological changes were more evident as days of demineralization increased; 7 days of immersion could be employed as an enamel erosive model. The pore count increased after the demineralization model, BD pores perimeter was heterogeneous, and AD varied according to the immersion period.
Morphological changes were more evident as days of demineralization increased.
Seven days of immersion could be employed as an enamel erosive model.
The initial porosity seems to be a determining factor for the final porousness.
The pore perimeter of the primary enamel varied according to the immersion period on the demineralization model.
Biodistribution of unmodified cardiosphere‐derived cell extracellular vesicles using single RNA tracing
Authors: Ciullo Alessandra , Li Chang , Li Liang , Ungerleider Korie C. , Peck Kiel , Marbán Eduardo , Ibrahim Ahmed G.E. 
Published: January 2022

AbstractExtracellular vesicles (EVs) are potent signalling mediators. Although interest in EV translation is ever‐increasing, development efforts are hampered by the inability to reliably assess the uptake of EVs and their RNA cargo. Here, we establish a novel qPCR‐based method for the detection of unmodified EVs using an RNA Tracer (DUST). In this proof‐of‐concept study we use a human‐specific Y RNA‐derived small RNA (YsRNA) we dub “NT4” that is enriched in cardiosphere‐derived cell small EVs (CDC‐sEVs). The assay is robust, sensitive, and reproducible. Intravenously administered CDC‐sEVs accumulated primarily in the heart on a per mg basis. Cardiac injury enhanced EV uptake in the heart, liver, and brain. Inhibition of EV docking by heparin suppressed uptake variably, while inhibition of endocytosis attenuated uptake in all organs. In vitro, EVs were uptaken more efficiently by macrophages, endothelial cells, and cardiac fibroblasts compared to cardiomyocytes. These findings demonstrate the utility of DUST to assess uptake of EVs in vivo and in vitro.