bluuebunny/crossref_metadata_2025_split huggingface dataset:
The cannabinoid receptor I (CB1) enhanced the osteogenic differentiation of BMSCs by rescue impaired mitochondrial metabolism function under inflammatory condition
Authors: Yan Wanhao , Li Le , Ge Lihua , Zhang Fengqiu , Fan Zhipeng , Hu Lei 
Published: January 2022

Abstract
Background
Periodontitis is a chronic infectious disease leading to bone resorption and periodontal tissue disruption under inflammatory stimulation. The osteogenic differentiation ability of mesenchymal stem cells (MSCs) is impaired under the inflammatory environment, which limits the effect of treatment. The cannabinoid receptor I (CB1)
is the main effector of the endogenous cannabinoid system (ECS), and our previous study verified that CB1 could enhance the osteo/dentinogenic differentiation of dental MSCs, which might be a target for alveolar bone regeneration. However, the effect of CB1 on the osteogenic differentiation of MSCs derived from bone remains unknown. In present study, we investigated the role and mechanism of CB1 on mitochondrial function and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) under inflammatory environment.

Methods
Alkaline phosphatase (ALP) activity, alizarin red staining, quantitative calcium analysis, and osteogenic markers were used to detect the osteogenic differentiation ability of BMSCs. Real-time RT-PCR and Western blot were used to detect the gene expression. Seahorse Cell Mito Stress Test was used to detect the oxygen consumption rate (OCR). JC-10 assay was used to determine the mitochondrial membrane potential (MMP).

Results
CB1 increased osteogenic differentiation potential and mitochondrial energy metabolism, including the OCR, MMP, and enhanced the expressions of Nrf1 and Nrf2 in hBMSCs without or with TNF-α or INF-γ stimulation. Then, the inhibitor of mitochondrial electron transport chain (ETC), rotenone (ROT), inhibited the osteogenic differentiation in hBMSCs, and CB1 could rescue ROT impaired osteogenic differentiation potentials of hBMSCs without or with TNF-α or INF-γ stimulation. Activation of ETC by Coenzyme Q10 (CoQ10) could restore the impaired osteogenic differentiation of hBMSCs by depletion of CB1 without or with TNF-α or INF-γ stimulation. Mechanismly, CB1 could activate the JNK signaling pathway, p38 MAPK signaling pathway, and inhibit the Erk1/2 signaling pathway.

Conclusions
The activating of CB1 enhanced the osteogenic differentiation by rescuing the mitochondrial metabolism function in hBMSCs under the inflammatory environment, suggesting that CB1 is a potential target for enhancing bone regeneration under the inflammatory environment.
Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae
Authors: Hu Gaowei , Chen Xue , Chu Wenhui , Ma Zhe , Miao Yingjie , Luo Xi , Fu Yongqian 
Published: January 2022

AbstractIn recent years, Klebsiella pneumoniae (KP) has caused disease outbreaks in different animals, resulting in serious economic losses and biosafety concerns. Considering the broad antibiotic resistance of KP, vaccines are the most effective tools against infection. However, there is still no KP vaccine available in the veterinary field. Our results indicate that the highly conserved outer membrane phosphoporin (PhoE) of KP is immunogenic in mice and elicits high titers of antibodies that were shown to be specific for PhoE by immunoblotting. Immunization with PhoE also induced robust cell-mediated immunity and elicited the secretion of high levels of IFN-γ and IL-4, suggesting the induction of mixed Th1 and Th2 responses. Sera from PhoE-immunized mice induced significantly higher complement-mediated lysis of KP cells than did sera from the PBS control mice. Finally, mice immunized with PhoE were significantly protected against KP challenge, with better survival and a reduced visceral bacterial load. Our data underscore the great potential of PhoE as a novel candidate antigen for a vaccine against KP infection.
A new mathematical model of multi-faced COVID-19 formulated by fractional derivative chains
Authors: Aldawish Ibtisam , Ibrahim Rabha W. 
Published: January 2022

AbstractIt has been reported that there are seven different types of coronaviruses realized by individuals, containing those responsible for the SARS, MERS, and COVID-19 epidemics. Nowadays, numerous designs of COVID-19 are investigated using different operators of fractional calculus. Most of these mathematical models describe only one type of COVID-19 (infected and asymptomatic). In this study, we aim to present an altered growth of two or more types of COVID-19. Our technique is based on the ABC-fractional derivative operator. We investigate a system of coupled differential equations, which contains the dynamics of the diffusion between infected and asymptomatic people. The consequence is accordingly connected with a macroscopic rule for the individuals. In this analysis, we utilize the concept of a fractional chain. This type of chain is a fractional differential–difference equation combining continuous and discrete variables. The existence of solutions is recognized by formulating a matrix theory. The solution of the approximated system is shown to have a minimax point at the origin.
Is a single portal venous phase in contrast-enhanced CT sufficient to detect metastases or recurrence in clear cell renal cell carcinoma? – a single-center retrospective study
Authors: Hagen Florian , Peisen Felix , Spogis Jakob , Mair Antonia , Nikolaou Konstantin , Stenzl Arnulf , Kruck Stephan , Bedke Jens , Kaufmann Sascha , Thaiss Wolfgang M. 
Published: January 2022

Abstract
Background
This study aims at describing the imaging features of the metastatic presentation of clear cell renal cell carcinoma (ccRCC) in arterial (AP) and portal venous phase (PVP) of contrast-enhanced-computed-tomography (CECT) during clinical follow-up (FU) and to evaluate the necessity of a dual phase approach for metastasis detection.

Methods
We identified a total of 584 patients that were diagnosed with ccRCC between January 2016 and April 2020. Inclusion criteria were histologically proven ccRCC with metastatic spread, proven by histology or interim follow-up of at least 2 years and follow-up CT examination with AP and PVP CECT including thorax/abdomen and pelvis. Exclusion criteria were defined by missing or incomplete CT-scans or lack of sufficient follow-up. CT studies of 43 patients with histologically proven ccRCCs were analyzed in retrospect. AP and PVP images were analyzed by two radiologists for metastases, two additional independent radiologists analyzed PVP images only. A 5-point Likert scale was used to evaluate the likelihood off the presence of metastasis. Imaging patterns of the metastases were analyzed visually.

Results
43 patients (16 female; mean age: 67±10 years) with recurrent ccRCC and metastatic disease were included. Three imaging patterns were observed (solid, heterogeneous or cystic metastases), which rarely exhibited calcifications (2%). All metastases showed hyperenhancement in AP and PVP. Inter-reader agreement was substantial (Fleiss’ κ 0.6–0.8, p<0.001). No significant differences in sensitivity or specificity between readers (AP and PVP images vs. PVP images only) were present (79.4-85.2%, 97.1-99.6%, p ≥ 0.05). The area under the receiver-operating-characteristic (ROC) curve was between 0.901and 0.922 for all four radiologists.

Conclusions
Similar rates for detection, sensitivity and specificity of metastasis and local recurrence in ccRCC were observed irrespective of using a dual-phase protocol with AP and PVP or a single PVP protocol only. Thus, a single-phase examination of PVP can be sufficient for experienced radiologists to detect metastatic disease in the follow-up of ccRCC patients.
Molecular structures and functional exploration of NDA family genes respond tolerant to alkaline stress in Gossypium hirsutum L.
Authors: Fan Yapeng , Zhang Yuexin , Rui Cun , Zhang Hong , Xu Nan , Wang Jing , Han Mingge , Lu Xuke , Chen Xiugui , Wang Delong , Wang Shuai , Guo Lixue , Zhao Lanjie , Huang Hui , Wang Junjuan , Sun Liangqing , Chen Chao , Ye Wuwei 
Published: January 2022

Abstract
Background
The internal NAD(P)H dehydrogenase (NDA) gene family was a member of the NAD(P)H dehydrogenase (ND) gene family, mainly involved in the non-phosphorylated respiratory pathways in mitochondria and played crucial roles in response to abiotic stress.

Methods
The whole genome identification, structure analysis and expression pattern of NDA gene family were conducted to analyze the NDA gene family.

Results
There were 51, 52, 26, and 24 NDA genes identified in G. hirsutum, G. barbadense, G. arboreum and G. raimondii, respectively. According to the structural characteristics of genes and traits of phylogenetic tree, we divided the NDA gene family into 8 clades. Gene structure analysis showed that the NDA gene family was relatively conservative. The four Gossypium species had good collinearity, and segmental duplication played an important role in the evolution of the NDA gene family. Analysis of cis-elements showed that most GhNDA genes contained cis-elements related to light response and plant hormones (ABA, MeJA and GA). The analysis of the expression patterns of GhNDA genes under different alkaline stress showed that GhNDA genes were actively involved in the response to alkaline stress, possibly through different molecular mechanisms. By analyzing the existing RNA-Seq data after alkaline stress, it was found that an NDA family gene GhNDA32 was expressed, and then theGhNDA32 was silenced by virus-induced gene silencing (VIGS). By observing the phenotype, we found that the wilting degree of silenced plants was much higher than that of the control plant after alkaline treatment, suggesting that GhNDA32 gene was involved in the response to alkaline stress.

Conclusions
In this study, GhNDAs participated in response to alkaline stress, especially NaHCO3 stress. It was of great significance for the future research on the molecular mechanism of NDA gene family in responding to abiotic stresses.
Embracing uncertainty: rethinking migration policy through pastoralists’ experiences
Authors: Maru Natasha , Nori Michele , Scoones Ian , Semplici Greta , Triandafyllidou Anna 
Published: January 2022

AbstractToday there is a disjuncture between migration flows that are complex, mixed and constantly evolving and the emerging global migration governance paradigm that seeks to impose clarity, certainty, regularity and order. Addressing the gap between policies and realities, this article explores lessons for migration policy and governance from mobile pastoralists’ experience. Using examples from human migration flows within and between Europe and Africa and insights from pastoral systems from India, Italy and Kenya, the article identifies important similarities between international migration and pastoral mobility. We focus on four interconnections: both international migration and pastoral mobility show multi-directional and fragmented patterns; both involve multiple, intersecting socio-economic, political, cultural and environmental drivers; both must respond to non-linear systems, where critical junctures and tipping points undermine clear prediction and forecasts, making social navigation and reliability management more useful concepts than risk-based prediction and control and finally for both uncertainty is not conceived of as a state of crisis but an inherent feature, pregnant with possibility and hope. Building on these four points, and drawing from pastoralists’ experiences, we propose some methodological, practical and policy reflections for bridging the disjuncture between migration realities on the ground and global migration governance policies and discourses.
Morbidity and mortality of COVID-19 negatively associated with the frequency of consanguineous marriages, an ecologic study
Authors: Saadat Mostafa 
Published: January 2022

AbstractBackgroundUnion between second cousins and closer relatives is called consanguineous marriage. Consanguineous marriage is associated with increased risk of autosomal recessive diseases and several multifactorial traits. In order to evaluate the association between prevalence/mortality of COVID-19 and the frequency of consanguineous marriage, the present ecologic study was carried out. For the present study, data of prevalence (per 106people) and mortality (per 106people) and number of performed laboratory diagnostic test (per 106people) of COVID-19 disease at four time points (December 2020; March, August and October 2021) of 65 countries were used.ResultsUnivariable correlation and generalized estimating equation analysis were used. In analysis, prevalence and mortality of COVID-19 were used as dependent variables and human development index, number of performed diagnosis test and the mean of inbreeding coefficient (α-value) were introduced into model as covariates, and time point was used as a factor in analysis. The square root (SR) of prevalence (P = 0.008) and SR-mortality (P < 0.001) of COVID-19 negatively associated with the log-transformed of α-value.ConclusionsThe present finding means that in countries with high levels of consanguineous marriages, the prevalence of COVID-19 and mortality due to COVID-19 were lower than countries having low level of marriage with relatives.
Release and intestinal translocation of chemicals associated with microplastics in an in vitro human gastrointestinal digestion model
Authors: Peters Ruud , de Jong Nadine , de Haan Laura , Wright Stephanie , Bouwmeester Hans 
Published: January 2022

AbstractThe global production of plastic currently exceeds 300 million tonnes per year. The extensive use of plastics and bad waste management has resulted in the presence of microplastics at different levels in the food production chain. From a chemical perspective, these microplastics are complex mixtures that contain multiple additives, such as plasticizers, flame retardants, stabilizers and pigments. Also other chemicals can be present in microplastics, including unreacted monomers, starting substances, and non-intentionally added substances. Finally, the microplastics may have adsorbed environmental contaminants. In this study we have used several types of microplastics, either from grinded beach litter or from frequently used food packing materials and pre-production samples. We quantified the chemical and metal release from these microplastics in worst case and physiological scenarios. We use a chemical extraction as worst case, and for the physiological scenarios we used an in vitro model of the human digestion and an in vitro model of the human intestinal epithelium. Subsequently all samples were analysed with sensitive ICP-MS, GCMS methods. We quantified 68 chemicals and 29 metals associated with a diversity of microplastics, some of these chemicals were also released in the luminal content of the human digestive tract under physiological conditions simulated in vitro. Only 22 chemicals reached the basolateral compartment of an in vitro intestinal epithelial model. From the ToxCast dataset we extracted 18 AOPs that were associated with the chemicals, that included AOPs associated with endocrine disruption. For a risk assessment of chemicals associated with microplastics more detailed data on oral microplastics exposure is needed, as well as more detailed toxicological studies on the hazards of both the individual and complex mixtures of the quantified chemicals.
COVID-19 pandemic effects on health worker’s mental health: Systematic review and meta-analysis
Authors: Aymerich Claudia , Pedruzo Borja , Pérez Jose Luís , Laborda Maria , Herrero Jon , Blanco Jorge , Mancebo Gonzalo , Andrés Lucía , Estévez Olatz , Fernandez Maitane , Salazar de Pablo Gonzalo , Catalan Ana , González-Torres Miguel Ángel 
Published: January 2022

AbstractBackgroundHealthcare workers (HCWs) exposed to coronavirus 19 (COVID-19) are at high risk of developing mental health concerns across several domains. The aim of this study is to determine the updated, global frequency of these outcomes.MethodsA multistep literature search was performed from database inception until March 1, 2021. PRISMA/MOOSE-compliant systematic review and PROSPERO protocol were used to identify studies reporting on depression, anxiety, acute stress, post-traumatic symptoms, insomnia, and burnout in HCWs exposed to COVID-19. A quantitative meta-analysis with random effects was conducted to analyze the proportion rate of the mental health disorders. Sensitivity analyses were performed to investigate the effect of the different continents and scales. Meta-regression analyses were conducted to examine the effect of gender, age, and work position.Results239 articles were included (n = 271,319 HCWs, mean age = 36.08 ± 8.33 (66.99% female). 33% HCWs exposed to COVID-19 reported depressive symptoms (95% confidence intervals [CI] = 28–38%), 42% anxiety features (95% CI = 35–48), 40% acute stress (95% CI = 32–47), 32% post-traumatic symptoms (95% CI = 26–37%), 42% insomnia (95% CI = 36–48), 37% burnout (95% CI = 31–42). Sensitivity analyses did not show statistically significant differences. Meta-regressions found a statistically significant lower prevalence of post-traumatic symptoms in Asia.ConclusionsHCWs exposed to COVID-19 were found to have a significant prevalence of mental health concerns in all domains analyzed. The effects of COVID-19 on HCWs’ mental health could be underestimated and the future consequences dismissed.
From fixer to facilitator: an interpretative phenomenological study of diabetes person-centred counselling and empowerment education
Authors: Findlay-White Florence , Dornan Tim , Davies Mark , Archer Alan , Kilvert Anne , Fox Charles 
Published: January 2022

Background: The purpose of this study is to explore the professional and personal experiences of multidisciplinary healthcare professionals during and following diabetes counselling and empowerment education. Methods: Everyone who had participated in a diabetes counselling and empowerment course between 2008-2016 was invited to respond to an online survey and follow-up telephone interview if willing. Interviews were recorded and transcribed verbatim. The research team used interpretative phenomenology to identify core themes from both the survey and telephone interviews and which captured the impact of empowerment education. Results: 22 doctors, nurses, dieticians, and psychologists completed an online questionnaire. 10 subsequently took part in telephone interviews. Empowerment education changed them from fixers to facilitators. Their transformation included a sense of becoming authentic, ‘being the way I want to be’ in clinical practice and becoming more self-reflective. This affected them personally as well as reinvigorating them professionally. Conclusions: The participants described a personal and professional journey of transformation that included discovering their person-centred philosophy. They adopted a consultation structure that empowered people with diabetes to care for themselves. It can be speculated that participants’ experience of transformation may also guard against professional burnout.
Screening patients with metastatic breast cancer for psychiatric disease: a cross sectional study
Authors: Basto Raquel , Caramujo Cecília , Ferreira Gomes Inês , Fraga Teresa , Correia Magalhães Joana , Sousa Maria João , de Jesus Sabrina , Monteiro Ana Raquel , Pestana-Santos Adriana , Carvalho Teresa , Albuquerque Emília , Sousa Gabriela 
Published: January 2022

Background: Cancer patients are at risk of developing some level of psychological dysregulation, depending on a series of variables related to the tumor, the environment, and the patient. In this context, it becomes important to define the difference between psychological and psychiatric disorder and to study its prevalence. The primary endpoint of this study is to evaluate the prevalence of psychiatric disorders in a sample of Portuguese patients with metastatic breast cancer and assess the relationship between these disorders and the characteristics of the oncological disease. Methods: Cross-sectional, single-center study with female patients diagnosed with metastatic breast carcinoma and under palliative treatment between November 2020 and May 2021. Psychiatric disorders were screened by applying and filling-out the MMSE, HADS, BSI, and WHOQoL-Bref instruments at the outpatient daycare unit when patients were present for treatmen. Results: A total of 91 female patients were included, median age 59.79 years. None of the patients had cognitive impairment (MMSE). HADS scale: 18.7% of the patients scored for anxiety and 17.6% for depression. The anxiety subscale score of > 8 (HADS) was related to ovarian function suppression (p<0.001), neoadjuvant therapy (p<0.001), and type of second-line of palliative treatment (p=0.024). The depression subscale score >8 (HADS) was related to the type of surgery performed (p= 0.022), molecular subtype of the tumor (p=0.020), and occurrence of grade 3-4 toxicities in the first (p=0.018), and third-line treatments (p=0.031). Conclusion: The screening of psychiatric disorders through the application of these scales by the medical oncology team may be able to aid in diagnosis and potentially lead to psychiatric referral and intervention at an earlier stage.
Turning on Dimensional Prominence in Decision Making: Experiments and a Model
Authors: Arad Ayala , Maltz Amnon 
Published: January 2022

Could introducing a tiny interest rate on positive balances of checking accounts affect investment decisions? We suggest, counterintuitively, that it might decrease allocations to checking accounts and increase riskless investments with higher returns. This violation of monotonicity is a potential outcome of a novel behavioral phenomenon that we formalize and investigate experimentally. It posits that even a small interest rate highlights or turns on the safe gains dimension, bumping up its decision weight while shrouding other considerations, such as liquidity. Consequently, choices may shift from the most liquid option, the checking account, to safe investments with superior returns. Our exploration of this phenomenon covers three different choice environments: investment decisions, social preferences, and choice under uncertainty. This paper was accepted by Yan Chen, behavioral economics and decision analysis.
Data-Driven Approximation Schemes for Joint Pricing and Inventory Control Models
Authors: Qin Hanzhang , Simchi-Levi David , Wang Li 
Published: January 2022

We study the classic multiperiod joint pricing and inventory control problem in a data-driven setting. In this problem, a retailer makes periodic decisions on the prices and inventory levels of a product that she wishes to sell. The retailer’s objective is to maximize the expected profit over a finite horizon by matching the inventory level with a random demand, which depends on the price in each period. In reality, the demand functions or random noise distributions are usually difficult to know exactly, whereas past demand data are relatively easy to collect. We propose a data-driven approximation algorithm that uses precollected demand data to solve the joint pricing and inventory control problem. We assume that the retailer does not know the noise distributions or the true demand functions; instead, we assume either she has access to demand hypothesis sets and the true demand functions can be represented by nonnegative combinations of candidate functions in the demand hypothesis sets, or the true demand function is generalized linear. We prove the algorithm’s sample complexity bound: the number of data samples needed in each period to guarantee a near-optimal profit is [Formula: see text], where T is the number of periods, and ϵ is the absolute difference between the expected profit of the data-driven policy and the expected optimal profit. In a numerical study, we demonstrate the construction of demand hypothesis sets from data and show that the proposed data-driven algorithm solves the dynamic problem effectively and significantly improves the optimality gaps over the baseline algorithms. This paper was accepted by J. George Shanthikumar, big data analytics.