bluuebunny/crossref_metadata_2025_split huggingface dataset:
Greenhouse gas emission of sugarcane irrigated with treated domestic sewage by subsurface drip in Southeast Brazil1
Authors: Barbosa Eduardo Augusto Agnellos , Gonçalves Ivo Zution , dos Santos Leonardo Nazário Silva , Nazário Aline Azevedo , Feitosa Daniel Rodrigues Cavalcante , do Carmo Janaína Braga , Matsura Edson Eiji 
Published: August 2022

AbstractReducing greenhouse gas emissions, reclaiming water for irrigation, and obtaining high agricultural yields are among the main agricultural challenges; therefore, this study aimed to analyse greenhouse gas emissions, including carbon dioxide, nitrous oxide, and methane emissions, in sugarcane irrigated with treated domestic sewage and with surface water, both with and without nutritional supplementation via fertigation, and in a nonirrigated control. Regarding the nonirrigated control with topdressing fertilization, nitrogen fertilization carried out by sewage or mineral sources decreased N‐N2O emissions by nearly 38% and 66%, respectively. C‐CH4 influx was found in all treatments with a nonsignificant contribution to the total emissions, representing average values between treatments near −54.56 g C‐CH4 ha−1. The crop irrigated with treated domestic sewage plus N minerals shows significantly the highest C‐CO2 equivalent emissions (15.12 Mg CO2eq ha−1), and the treatment irrigated with sewage without N minerals showed the highest relation between the yield of theoretical recoverable sugar and C‐CO2 equivalent emissions (2.63 Mg recoverable sugar Mg−1 CO2eq ha−1). The treatments using sewage or with surface water irrigation, both fertigated, increased the stalk biomass and sugar yield, with a higher yield of theoretical recoverable sugar.
Electrospun polylactic acid scaffolds with strontium‐ and cobalt‐doped bioglass for potential use in bone tissue engineering applications
Authors: de Souza Joyce Rodrigues , Kukulka Elisa Camargo , Araújo Juliani Caroline Ribeiro , Campos Tiago Moreira Bastos , do Prado Renata Falchete , de Vasconcellos Luana Marotta Reis , Thin Gilmar Patrocínio , Borges Alexandre Luiz Souto 
Published: August 2022

AbstractThe development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol–gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA‐BG), 4% bioglass‐doped strontium (PLA‐BGSr) and 4% bioglass‐doped cobalt (PLA‐BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro–Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal–Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano‐sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering.
Effect of artificial skin membrane on the expression of miR‐155 and miR‐506‐3p in patients with second‐degree burns
Authors: Li Fei , Wan Dong Wu , Hu Jun , Qin Runhe 
Published: August 2022

AbstractObjectiveTo investigate the effect of artificial skin on the expression of miR‐155 and miR‐506‐3p in patients with second‐degree burns.MethodsThe study subjects included 50 patients with second‐degree burns treated from July 2019 to July 2021. The control group received routine nursing, while the research group received both routine and artificial skin intervention simultaneously. The changes in wound tissue fibrosis and prognosis were observed. The expression levels of miR‐155 and miR‐506‐3p and their downstream regulatory factors were detected and correlated with the rehabilitation of patients after artificial skin treatment.ResultsAfter treating second‐degree burns with artificial skin membranes, the patient's wound tissue fibrosis and inflammation level improved. At the same time, the expression levels of miR‐155 and miR‐506‐3p in related tests were higher than those in patients with available treatment.ConclusionThe effect of artificial skin membrane on the wound healing of second‐degree burn patients may be realized by influencing the expression levels of miR‐155 and miR‐506‐3p and their related signaling pathways.
Misdiagnosis of a patient with congenital dysfibrinogenemia: A case report and literature review
Authors: Chen Xinyan , Yan Jie , Xiang Liqun , Lin Faquan 
Published: August 2022

AbstractBackgroundWe reported a patient with congenital dysfibrinogenemia who was misdiagnosed and reviewed relevant literature, in order to discuss the methods to reduce misdiagnosis.MethodsA 23‐year‐old pregnant woman was found to be with low fibrinogen in antenatal examination at another province teaching hospital, who was misdiagnosed to have hypofibrinogenemia. Fibrinogen infusion or cryoprecipitation was recommended if necessary. The patient came to our hospital for further diagnosis and treatment considering the safety of herself and the fetus. We examined the coagulation function and gene sequencing of the pregnant woman and her family members.ResultsFibrinogen (Clauss method) was significantly reduced in the patient and her mother, while the level of fibrinogen (PT‐derived method) was normal. Thrombin time was prolonged. Heterozygous mutation site was found in exon 2 of the FGA gene, c.104G > A(p.Arg35His).ConclusionWhen the fibrinogen (Clauss method) is significantly reduced and the thrombin time is prolonged, PT‐derived method and the investigation of family coagulation function should be added, which can be used to diagnose and distinguish congenital dysfibrinogenemia from hypofibrinogenemia.
Associations between ATG16L1 gene polymorphism and antineutrophil cytoplasmic antibody‐associated vasculitis in the Chinese Guangxi population: A case–control study
Authors: Feng Bing‐Mei , Xue Chao , Huang Hai‐Zheng , Lu Yu‐gan , Feng Tao , Huang Xue‐Yi , Li Wei 
Published: August 2022

AbstractBackgroundAntineutrophil cytoplasmic antibody (ANCA)‐associated vasculitis (AAV) is an autoimmune disease often accompanied by rapidly progressive renal failure, and the genetic background is still unknown. Our study was performed to test whether autophagy‐related 16 like 1 (ATG16L1) rs4663402 and rs4663396 single nucleotide polymorphisms (SNPs) were associated with AAV in the Chinese Guangxi population.MethodsOne hundred seventy seven unrelated AAV patients and 216 healthy controls were included in this case–control study. Multiplex polymerase chain reaction combined with high‐throughput sequencing was used for typing, and SNPStats and SHEsis were used for association analysis, pairwise linkage disequilibrium, and haplotype analysis.Resultsrs4663402 and rs4663396 were in Hardy–Weinberg equilibrium in AAV and control groups. The frequencies of rs4663402 AA, AT, and TT genotypes were 82.5%, 16.9%, and 0.6%, respectively, in patients with AAV, and 83.5%, 16.2%, and 0.5%, respectively, in controls. The frequencies of rs4663396 CC, CT, and TT genotypes were 63.8%, 33.9%, and 2.3%, respectively, in patients with AAV, and 69.2%, 26.6%, and 4.2%, respectively, in controls. Haplotype analysis revealed two SNPs in a single haplotype block (D′ = 1.0). Our logistic regression adjusted for sex and age showed no association between rs4663402 and rs4663396 and the risk for AAV in genetic models (p > 0.05). However, ATG16L1 rs4663396 CC and CT + TT genotypes exhibited statistically significant differences in the incidence of arthralgia (p = 0.03).ConclusionsOur results indicated that ATG16L1 rs4663402 and rs4663396 polymorphisms were not associated with AAV in the Chinese Guangxi population. ATG16L1 rs4663396 CT + TT genotype may be associated with arthralgia.
Functional analysis of HECA variants identified in congenital heart disease in the Chinese population
Authors: Li Ting , Wu Yao , Chen Wei‐Cheng , Xue Xing , Suo Mei‐Jiao , Li Ping , Sheng Wei , Huang Guo‐Ying 
Published: August 2022

AbstractBackgroundCongenital heart disease (CHD) is a class of cardiovascular defects that includes septal defects, outflow tract abnormalities, and valve defects. Human homolog of Drosophila headcase (HECA) is a novel cell cycle regulator whose role in CHD has not been elucidated. This is the first study to determine the frequency of HECA mutations in patients with CHD and the association between HECA variants and CHD.MethodsIn this study, we identified a candidate gene, HECA, by whole‐exome sequencing of an atrial septal defect family. To investigate the association between HECA variants and CHD risk, targeted exon sequencing was conducted in 689 individuals with sporadic CHD. We further analyzed the effect of HECA gene abnormalities on cardiomyocyte phenotype behavior and related signaling pathways by Western blotting, reverse transcription‐quantitative polymerase chain reaction, and scratch assay.ResultsWe found a novel de novo mutation, c.409_410insA (p. W137fs), in the HECA gene and identified five rare deleterious variants that met the filtering criteria in 689 individuals with sporadic CHD. Fisher's exact test revealed a significant association between HECA variations and CHD compared with those in gnomADv2‐East Asians(p = 0.0027). Further functional analysis suggested that the variant p. W137fs resulted in a deficiency of the normal HECA protein, and HECA deficiency altered AC16 cell cycle progression, increased cell proliferation, and migration, and promoted the activation of the PDGF‐BB/PDGFRB/AKT pathway.ConclusionsOur study identified HECA and its six rare variants, expanding the spectrum of genes associated with CHD pathogenesis in the Chinese population.
Antibiotic resistance: The challenges and some emerging strategies for tackling a global menace
Authors: Chinemerem Nwobodo David , Ugwu Malachy Chigozie , Oliseloke Anie Clement , Al‐Ouqaili Mushtak T. S. , Chinedu Ikem Joseph , Victor Chigozie Uchenna , Saki Morteza 
Published: August 2022

AbstractBackgroundAntibiotic resistance is currently the most serious global threat to the effective treatment of bacterial infections. Antibiotic resistance has been established to adversely affect both clinical and therapeutic outcomes, with consequences ranging from treatment failures and the need for expensive and safer alternative drugs to the cost of higher rates of morbidity and mortality, longer hospitalization, and high‐healthcare costs. The search for new antibiotics and other antimicrobials continues to be a pressing need in humanity's battle against bacterial infections. Antibiotic resistance appears inevitable, and there is a continuous lack of interest in investing in new antibiotic research by pharmaceutical industries. This review summarized some new strategies for tackling antibiotic resistance in bacteria.MethodsTo provide an overview of the recent research, we look at some new strategies for preventing resistance and/or reviving bacteria's susceptibility to already existing antibiotics.ResultsSubstantial pieces of evidence suggest that antimicrobials interact with host immunity, leading to potent indirect effects that improve antibacterial activities and may result in more swift and complete bactericidal effects. A new class of antibiotics referred to as immuno‐antibiotics and the targeting of some biochemical resistance pathway components including inhibition of SOS response and hydrogen sulfide as biochemical underlying networks of bacteria can be considered as new emerging strategies to combat antibiotic resistance in bacteria.ConclusionThis review highlighted and discussed immuno‐antibiotics and inhibition of SOS response and hydrogen sulfide as biochemical underlying networks of bacteria as new weapons against antibiotic resistance in bacteria.
Impact of muscle mass on survival of patients with hepatocellular carcinoma after liver transplantation beyond the Milan criteria
Authors: Beumer Berend R. , van Vugt Jeroen L.A. , Sapisochin Gonzalo , Yoon Peter , Bongini Marco , Lu Di , Xu Xiao , De Simone Paolo , Pintore Lorenzo , Golse Nicolas , Nowosad Malgorzata , Bennet William , Tsochatzis Emmanouil , Koutli Evangelia , Abbassi Fariba , Claasen Marco P.A.W. , Merli Manuela , O'Rourke Joanne , Gambato Martina , Benito Alberto , Majumdar Avik , Tan Ek Khoon , Ebadi Maryam , Montano‐Loza Aldo J. , Berenguer Marina , Metselaar Herold J. , Polak Wojciech G. , Mazzaferro Vincenzo , IJzermans Jan N.M. ,   Collaborators
Published: August 2022

AbstractBackgroundAccess to the liver transplant waitlist for patients with hepatocellular carcinoma (HCC) depends on tumour presentation, biology, and response to treatments. The Milan Criteria (MC) represent the benchmark for expanded criteria that incorporate additional prognostic factors. The purpose of this study was to determine the added value of skeletal muscle index (SMI) in HCC patients beyond the MC.MethodPatients with HCC that were transplanted beyond the MC were included in this retrospective multicentre study. SMI was quantified using the Computed Tomography (CT) within 3 months prior to transplantation. Cox regression models were used to identify predictors of overall survival (OS). The discriminative performance of SMI extended Metroticket 2.0 and AFP models was also assessed.ResultsOut of 889 patients transplanted outside the MC, 528 had a CT scan within 3 months prior to liver transplantation (LT), of whom 176 (33%) were classified as sarcopenic. The median time between assessment of the SMI and LT was 1.8 months (IQR: 0.77–2.67). The median follow‐up period was 5.1 95% CI [4.7–5.5] years, with a total of 177 recorded deaths from any cause. In a linear regression model with SMI as the dependent variable, only male gender (8.55 95% CI [6.51–10.59], P < 0.001) and body mass index (0.74 95% CI [0.59–0.89], P < 0.001) were significant. Univariable survival analysis of patients with sarcopenia versus patients without sarcopenia showed a significant difference in OS (HR 1.44 95% CI [1.07 − 1.94], P = 0.018). Also the SMI was significant (HR 0.98 95% CI [0.96–0.99], P = 0.014). The survival difference between the lowest SMI quartile versus the highest SMI quartile was significant (log‐rank: P = 0.005) with 5 year OS of 57% and 71%, respectively. Data from 423 patients, describing 139 deaths, was used for multivariate analysis. Both sarcopenia (HR 1.45 95% CI [1.02 − 2.05], P = 0.036) and SMI were (HR 0.98 95% CI [0.95–0.99], P = 0.035) significant. On the survival scale this translates to a 5 year OS difference of 11% between sarcopenia and no sarcopenia. Whereas for SMI, this translates to a survival difference of 8% between first and third quartiles for both genders.ConclusionsOverall, we can conclude that higher muscle mass contributes to a better long‐term survival. However, for individual patients, low muscle mass should not be considered an absolute contra‐indication for LT as its discriminatory performance was limited.
Patella height influences patellofemoral contact and kinematics following cruciate‐retaining total knee replacement
Authors: Tischer Thomas , Geier Andreas , Lutter Christoph , Enz Andreas , Bader Rainer , Kebbach Maeruan 
Published: August 2022

AbstractThe role of patella height is discussed controversially in total knee arthroplasty (TKA). Therefore, this computational study aims to systematically analyze the biomechanical effect of different patella heights on patellofemoral (PF) forces and kinematics after cruciate‐retaining (CR) TKA. We implemented a CR bicondylar TKA with a dome patellar button in a validated dynamic musculoskeletal multibody model of a male human knee joint. Retropatellar dynamics (contact force [N], shear force [N], patellar shift [mm], tilt [°], and rotation [°]) were evaluated during dual‐limb squat motion (flexion from 0° to 90°) with simulated active muscle forces and the effects of different patella heights (Blackburne‐Peel [BP] ratio of 0.39, 0.49, 0.65, 0.85, 1.01, and 1.1 were systematically examined). As active knee flexion increased, PF contact force also increased. Patella alta (BP = 1.1) resulted in higher PF contact forces compared to normal patella height (BP = 0.65) by up to 16%. Contrarily, patella baja was associated with decreased PF forces by 7%. Compared to patella baja (BP = 0.39), patella alta (BP = 1.1) considerably increased the contact force by up to 25%. Different patellar heights mainly affected PF shear forces during early knee flexion. Concerning PF kinematics, patella alta (BP = 1.1) yielded a greater lateral tilt of more than 4° and higher patellar rotation by up to 3° during deep knee flexion, compared to normal patella height (BP = 0.65). Our computational study indicates that patella alta is associated with the highest PF contact and shear force after the implantation of a CR bicondylar TKA. This should be considered in PF disorders following TKA.
Greater hip abductor size in prearthritic patients with developmental dysplasia of the hip versus femoroacetabular impingement
Authors: Payne Emma R. , Harris Michael D. , Harris‐Hayes Marcie , Nahal Chadi , Kamenaga Tomoyuki , Clohisy John C. , Pascual‐Garrido Cecilia 
Published: August 2022

AbstractDevelopmental dysplasia of the hip (DDH) and femoroacetabular impingement (FAI) are common hip pathologies and important risk factors for osteoarthritis, yet the disease mechanisms differ. DDH involves deficient femoral head coverage and a shortened abductor moment arm, so this study hypothesized that the cross‐sectional area (CSA) of the gluteus medius/minimus muscle complex and the stabilizing iliocapsularis muscle would be larger in DDH versus FAI, without increased fatty infiltration. A longitudinal cohort identified prearthritic patients with DDH or FAI who underwent imaging before surgery. Patients with DDH and FAI (Cam, Pincer, or Mixed) were 1:1 matched based on age, sex, and body mass index. Magnetic resonance imaging was used to measure the gluteus medius/minimus complex and iliocapsularis in two transverse planes. Amira software was used to quantify muscle and noncontractile tissue. Paired samples t‐tests were performed to compare muscle size and composition (p < 0.05). There were no differences in the iliocapsularis muscle. Patients with DDH had significantly larger CSA of the gluteus medius/minimus complex at both transverse planes, and the noncontractile tissue proportion did not differ. The mean difference in overall muscle CSA at the anterior inferior iliac spine was 4.07 ± 7.4 cm2 (p = 0.005), with an average difference of 12.1%, and at the femoral head this was 2.40 ± 4.37 cm2 (p = 0.004), with an average difference of 20.2%. This study reports a larger CSA of the gluteus medius/minimus muscle complex in DDH compared to FAI, without a difference in noncontractile tissue, indicating increased healthy muscle in DDH.
Raman study of quench products of alkaline carbonate melt at 3 and 6 GPa: Link to the pressure of origin
Authors: Arefiev Anton , Shatskiy Anton , Bekhtenova Altyna , Litasov Konstantin 
Published: August 2022

AbstractInclusions of former alkaline carbonate melts occur in igneous minerals, mantle xenoliths, and diamonds from kimberlites and lamproites. However, the identification of most daughter phases remains undetermined owing to the lack of their Raman spectra. Yet, the phase compositions of the alkali carbonate melt at different pressure are interesting to know because Na‐Ca, Na‐Mg, K‐Ca, and K‐Mg double carbonates have a number of pressure‐induced phase transitions. Here, we studied the quench products of K‐Ca, K‐Mg, and Na‐Ca carbonate melts obtained at 3 and 6 GPa. Scanning electron microscopy coupled with energy‐dispersive spectroscopy with silicon drift detector (EDS‐SDD) and Raman spectroscopy were employed for phase characterization. The following carbonates were detected among the quench products: γ‐Na2CO3, Na4Ca(CO3)3 (Ia3d), Na2Ca3(CO3)3 (P1n1), K2Ca(CO3)2 (
) bütschliite, disordered K2Ca3(CO3)4 (Pnam), K2Mg(CO3)2 (
), aragonite, magnesite at 6 GPa; and CaCO3‐bearing K2CO3, K2Ca(CO3)2 (
) bütschliite, K2Ca2(CO3)3 (R3), and ordered K2Ca3(CO3)4 (P212121) at 3 GPa. The results indicate that the quench products of carbonate melt consist of carbonates thermodynamically stable at the pressure of quenching. Thus, the daughter phases in carbonatitic inclusions could be identified by obtained Raman spectra and reflect the pressure conditions of their entrapment.
Bayesian sparse modeling to identify high‐risk subgroups in meta‐analysis of safety data
Authors: Qi Xinyue , Zhou Shouhao , Wang Yucai , Peterson Christine 
Published: August 2022

AbstractMeta‐analysis allows researchers to combine evidence from multiple studies, making it a powerful tool for synthesizing information on the safety profiles of new medical interventions. There is a critical need to identify subgroups at high risk of experiencing treatment‐related toxicities. However, this remains quite challenging from a statistical perspective as there are a variety of clinical risk factors that may be relevant for different types of adverse events, and adverse events of interest may be rare or incompletely reported. We frame this challenge as a variable selection problem and propose a Bayesian hierarchical model which incorporates a horseshoe prior on the interaction terms to identify high‐risk groups. Our proposed model is motivated by a meta‐analysis of adverse events in cancer immunotherapy, and our results uncover key factors driving the risk of specific types of treatment‐related adverse events.
Existence and uniqueness of solutions of differential equations with respect to non‐additive measures
Authors: Singh Negi Shekhar , Torra Vicenç 
Published: August 2022

By taking Sugeno‐derivative into account, first, we investigate the existence of solutions to the initial value problems (IVP) of first‐order differential equations with respect to non‐additive measure (more precisely, distorted Lebesgue measure). It particularly occurs in the mathematical modeling of biology. We begin by expressing the differential equation in terms of ordinary derivative and the derivative with respect to the distorted Lebesgue measure. Then, by using the fixed point theorem on cones, we construct an operator and prove the existence of positive non‐decreasing solutions on cones in semi‐order Banach spaces. In addition, we also use Picard–Lindelöf theorem to prove the existence and uniqueness of the solution of the equation.Second, we investigate the existence of a solution to the boundary value problem (BVP) on cones with integral boundary conditions of a mix‐order differential equation with respect to non‐additive measures. Moreover, the Krasnoselskii fixed point theorem is also applied to both BVP and IVP and obtains at least one positive non‐decreasing solution. Examples with graphs are provided to validate the results.