bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence-Based Design Process for Nutrition-Sensitive Agriculture Interventions: A Case Study of the Advancing Local Dairy Development Programme in Nigeria
Authors: Ojo Ayodeji , Adeyemi Olutayo , Kayode Fisayomi , Oyebamiji Olumide , Onabolu Adeyinka , Grema Audu , MacNaughtan Kristen , Ajieroh Victor 
Published: December 2022

Background: Nigeria is a significant contributor to the global burden of chronic undernutrition in children under 5 years of age. The undernutrition challenge constrains the productivity of rural households. This study was carried out among beneficiaries of the productivity-enhancing Nigerian Dairy Development Programme (NDDP) to guide incorporation of nutrition into the successor program—Advancing Local Dairy Development in Nigeria (ALDDN). Therefore, this study contributes to the literature about operationalizing nutrition-sensitive agricultural frameworks. Objective: The study sought to determine potential entry points for improving the nutrition of smallholder dairy farmers (≤ 5 milk cows) with a focus on food system entry points. Methods: Primary data were collected from 514 smallholder dairy households in Oyo and Kano states of Nigeria. Both quantitative and qualitative data were collected. Quantitative data collected included minimum dietary diversity for women, nutrition status, and socioeconomic characteristics of households. Qualitative data included information about sources of food, markets, and other food system characteristics. Quantitative data were analysed using descriptive statistical tools and correlation analysis, while common themes were identified from qualitative data. Conclusions: The study showcased men’s important role in food purchase and consumption, particularly in Kano. Increasing opportunities and the ability of women to use nutrition education appeared necessary for translating knowledge into improved practices. The own consumption (i.e., the portion of the milk kept for households’ consumption as well as diversification of foods produced for consumption); income; women’s empowerment; and environmental sanitation pathways seemed to be the most feasible pathways for improving nutrition within the context of the study population.
Indian Experience of Managing Impossible Trinity, Growth and Possible Tradeoff Between FDI and FIIs: Nature of Capital Inflows Matter
Authors: Padhi Satya Prasad 
Published: December 2022

The current focus emphasizes the difficult choices faced by Indian management of the impossible trinity to manage import-led growth. The management highlights two mechanisms by which FDI (foreign direct investment) flows are easily substituted for short-run FII (foreign institutional investment) flows. First, GDP and its growth in an open economy framework and the associated international trade participation can clarify this substitution prospect. Specifically, this study develops reasoning whereby imports under the trilemma management can give some decisive advantage to FII at the cost of FDI to manage Indian import-led growth. Second, it underlines how the related policies, especially the sterilization interventions, constrain the interactions of higher domestic investments (domestic savings), spillover impacts of imports of intermediate and capital goods, and specialized FDI. If so, the Indian economy’s import-led growth can indicate FII and FDI tradeoffs. The study uses the ARDL regression method. The results show that the Indian policy-led macro environment can limit the scope of specialized FDI that aims to induce advanced domestic investment, exports, and FDI interactions. India’s focus on import-led growth thereby underpins how FII substitutes FDI. JEL Classification F21, F3, F4, O11, O14
An improved two-steps method for secondary in-the-bag intraocular lens implantation in aphakia eye
Authors: Li Xiaomeng , Luo Dawei , Gu Chufeng , Lhamo Thashi , Draga Deji , Zheng Zhi , Qiu Qinghua 
Published: December 2022

Purpose To describe an improved technique for secondary IOL implantation in constricted capsular bag in aphakic eyes. Setting This study was designed and carried out in the department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital). Design This is a retrospective study which evaluate the post-operative effect of the two-steps secondary IOL implantation. Method 21 eyes of 21 patients who underwent primary cataract surgery from June 2020 to September 2020 were enrolled. Two-steps IOL implantation was performed. Patients were followed up with ophthalmic examinations. Results Of all 21 eyes, the capsular bags were reopened and the IOL were implanted into the bag. During follow-up, the mean age was 62.3 ± 7.3 (range, 44–81) years and mean interval between the primary surgery and secondary IOL implantation was 23.5 ± 9.6 (range, 4.1–78.3) weeks. A marked improvement of postoperative mean BCVA was noticed (pre 20/84 Snellen, 0.62 ± 0.26 logMAR equivalent, and post 20/44 Snellen, 0.34 ± 0.33 logMAR equivalent; p = 0.001). The mean SE also improved from + 10.32 ± 5.05D at baseline to −2.68 ± 1.36D at the last follow-up time ( p < 0.001). The mean IOP showed no significant difference (pre 17.62 ± 4.66 mmHg, post16.84 ± 4.73 mmHg; p = 0.6860) and the corneal endothelial cell density decreased about 11.04 ± 10.84% (from 2483 ± 218.43 cells/mm2 to 2190 ± 361.36 cells/mm2 p = 0.0029) Conclusion The two-steps capsular bag reopening method can be achieved in majority of cases with stable IOL position and better visual outcome.
Histologic healing and factors associated with complete remission following conventional treatment in ulcerative colitis
Authors: Steinsbø Øyvind , Carlsen Arne , Aasprong Ole Gunnar , Aabakken Lars , Tvedt-Gundersen Espen , Bjørkhaug Steinar , Gjerde Rune , Normann Karlsen Lars , Grimstad Tore 
Published: December 2022

Background: Endoscopic and histological activity scores in ulcerative colitis (UC) are associated with clinical outcomes and have become important targets of clinical trials. However, these endpoints have been scarcely investigated in patients receiving only conventional treatment. Objective: We aimed to assess the deep and complete remission rates after 3 months of conventional treatment in patients with newly diagnosed UC with moderate to severe endoscopic activity. We also aimed to investigate whether selected clinical and biochemical variables at baseline were associated with complete remission status after 3 months. Design: This was a prospective cohort study. Methods: Newly diagnosed patients with active UC commencing 5-aminosalicylate, corticosteroid, and/or azathioprine treatment were consecutively included. Clinical, biochemical, endoscopic, and histological data were collected at baseline and after 3 months. Rates of clinical remission (Partial Mayo Score ⩽ 2), mucosal healing (Mayo Endoscopic Score ⩽ 1), and histologic healing (Nancy Index ⩽ 1) were determined. Deep remission was assessed as clinical remission plus mucosal healing and complete remission as deep remission plus histologic healing. Predictors of complete remission were identified by logistic regression. Results: A total of 180 patients were included in the study. Deep remission and complete remission occurred in 62.8% and 42.2% of patients, respectively. Thus, of patients in deep remission one-third had persistent histologic activity. Histologic activity in mucosally healed patients was associated with higher symptom scores and faecal calprotectin levels. Of baseline variables, less endoscopic distribution and disease activity showed strongest association with achieving complete remission, and limited distribution in combination with moderate activity gave highest odds for complete remission (odds ratio: 4.1, 95% confidence interval: 7.69–2.18). Conclusion: In patients with mucosal healing, persistent histologic activity was a common finding and was associated with increased disease activity. Pancolitis and severe inflammatory activity at baseline were associated with lower complete remission rates.
Neutrophil–lymphocyte ratio as an inflammatory biomarker of diabetic nephropathy among type 2 diabetes mellitus patients: A comparative cross-sectional study
Authors: Gurmu Mesfin Zewude , Genet Solomon , Gizaw Solomon Tebeje , Feyisa Teka Obsa , Gnanasekaran Natesan 
Published: December 2022

Objective: This study aimed to determine the neutrophil–lymphocyte ratio (NLR) as an inflammatory biomarker among type 2 diabetes mellitus (T2DM) patients with diabetic nephropathy (DN). Methods: A comparative cross-sectional study design was conducted on 199 T2DM patients attending Bole 17 Health Center, Addis Ababa, Ethiopia. The urine albumin test was done by the MICRAL-II test strip. Fasting blood sugar was measured by a glucometer. Complete blood count was analyzed using an automated hematology analyzer (HUMAN GmbH, Wiesbaden, Hesse, Germany). The student’s t-test, a chi-square test, and Pearson correlation were applied to analyze the data. Results: Out of the 199 diabetes mellitus patients, 45 (22.6%) and 154 (77.4%) were found with DN and without DN, respectively. Interestingly, the mean NLR value (2.66 ± 0.49) was found significantly higher in diabetic patients with DN compared to the mean NLR (1.65 ± 0.20) in diabetes patients without DN ( p < 0.0001). The NLR showed positive significant correlation with variables such as age ( r = 0.162, p = 0.023), duration of disease ( r = 0.52, p < 0.0001), absolute neutrophil count ( r = 0.712, p < 0.0001), total white blood cell count ( r = 0.162, p = 0.022), systolic blood pressure ( r = 0.338, p < 0.0001), and diastolic blood pressure ( r = 0.731, p < 0.0001). On the other hand, negatively significant correlation was found between NLR and absolute lymphocyte count ( r = −0.770, p < 0.000). Conclusion: The NLR was significantly increased in T2DM patients with DN, suggesting that inflammation and endothelial dysfunction could be an integral part of the pathogenesis of DN, and therefore, this ratio may be considered as a predictor and a prognostic biomarker of DN.
Critical stenosis of left main coronary artery as a late presentation after transcatheter aortic valve replacement: A case report and review literature
Authors: Alsaddah Jadan , Alenezi Abdullah , Maadarani Ossama , Bitar Zouheir , Alfadhli Rahaf 
Published: December 2022

Summary Transcatheter aortic valve replacement (TAVR) is an alternative treatment for selected patients with severe aortic valve stenosis who are at high risk for conventional surgery. Critical stenosis of left main coronary artery as a late complication after TAVR is quite rare.
Enhancing the Occurrence Variance Reporting System Implementation at one of the Hospitals of the Egyptian Universal Health Insurance
Authors: El-Sayed Aly Rasha Ibrahim , Ahmed Mohamed ELBahlawan Gehan , Aly Maha Ramadan , Mohamed Elsayed Boshra Karem 
Published: December 2022

Background Identifying, reporting, measuring, and tracking events provide an opportunity to study system issues, motivate learning, measure the frequency and severity of events, and manage high-risk ones which refer to a safety culture that is focused on valuing the input of working staff and improving the quality of care. Aim Enhance the implementation of the occurrence variance reporting (OVR) system at the Obstetrics and Gynecological Hospital in Port Said Governorate, Egypt. Design A quasi-experimental research design for one group (pre–posttest) and a mixed-methods approach was conducted in this study. Method This study was carried out at an Obstetrics and Gynecological Hospital in Port Said Governorate, Egypt. Study subjects included a convenient sample of 100 doctors and nurses. The study used three tools: OVR Knowledge, Attitude, and Practice (KAP) questionnaire, the OVR trend analysis clinical audit checklist, and barriers that hinder staff to report patient safety events through two open-ended questions. Results Significant improvements were detected in the OVR system post-program implementation than pre-program implementation phase. A statistically significant increase in nurses’ and doctors’ total knowledge score from 0.74 to 3.39 and a statistically significant decrease in nurses’ and doctors’ total negative attitude score from 3.87 to 3.27. Also, a statistically significant increase in total practice score from 2.35 to 2.45. Conclusion There were significant improvements in the hospital OVR system postprogram implementation than preprogram implementation. Relevance to clinical practice To maintain performance and make sure that the original result is not lost, the health care facilities should emphasize the ongoing monthly and quarterly monitoring and analysis of data. Meetings, lectures, and training sessions are used for ongoing education.
Mouth opening in systemic sclerosis: Its course over time, determinants and impact on mouth handicap
Authors: Khidir Sarah J H , Boonstra Maaike , Bergstra Sytske Anne , Boerrigter Gerry W M , Voogt-van der Harst Elles M , Ninaber Maarten K , Ajmone Marsan Nina , Huizinga Tom W J , van der Helm-van Mil Annette H M , de Vries-Bouwstra Jeska K 
Published: December 2022

Objective: Decreased maximal mouth opening is a common and disabling manifestation in systemic sclerosis patients. We aimed to study the course of maximal mouth opening, determinants of smaller maximal mouth opening over time and the burden of smaller maximal mouth opening on mouth handicap. Methods: Consecutive systemic sclerosis patients participating in the prospective Leiden Combined Care in systemic sclerosis cohort were included. Annual clinical assessment included maximal mouth opening measurement and mouth handicap evaluation (Mouth Handicap in Systemic Sclerosis scale). Presence of microstomia (maximal mouth opening < 30 mm) was studied. Maximal mouth opening over time was assessed on group level and for all patients individually. Baseline characteristics were analysed for their association with smaller maximal mouth opening over time (linear mixed-effects models). Furthermore, cross-sectional association between maximal mouth opening with Mouth Handicap in Systemic Sclerosis scale was assessed (linear regression analysis). Results: A total of 382 systemic sclerosis patients were studied with median follow-up time of 2.0 years (interquartile range = 0.0–3.0). At baseline, mean maximal mouth opening was 42.2 ± 8.0 mm and 7% suffered from microstomia. Annual decrease of > 5.0 mm in maximal mouth opening during follow-up occurred in 63 patients and was accompanied by increase in disease severity. Disease characteristics at baseline independently predictive for smaller maximal mouth opening over time were: more extended skin subtype; peripheral vasculopathy; pulmonary, renal and gastrointestinal involvement. Smaller maximal mouth opening was significantly associated with more reported mouth handicap. Conclusion: The course of maximal mouth opening is stable in a majority of systemic sclerosis patients. Still, maximal mouth opening over time was smaller in patients with more severe organ involvement. Although microstomia was infrequent, a smaller maximal mouth opening was significantly associated with more mouth handicap, indicating the importance to address maximal mouth opening in routine care of systemic sclerosis patients.
Identification of fish species through tRNA-based primer design
Authors: Wu Ting-Hui , Yang Cing-Han , Pai Tun-Wen , Ho Li-Ping , Wu Jen-Leih , Chou Hsin-Yiu 
Published: December 2022

Abstract
Background
The correct establishment of the barcode classification system for fish can facilitate biotaxonomists to distinguish fish species, and it can help the government to verify the authenticity of the ingredients of fish products or identify unknown fish related samples. The Cytochrome c oxidation I (COI) gene sequence in the mitochondria of each species possesses unique characteristics, which has been widely used as barcodes in identifying species in recent years. Instead of using COI gene sequences for primer design, flanking tRNA segments of COI genes from 2618 complete fish mitochondrial genomes were analyzed to discover suitable primers for fish classification at taxonomic family level. The minimal number of primer sets is designed to effectively distinguish various clustered groups of fish species for identification applications. Sequence alignment analysis and cross tRNA segment comparisons were applied to check and ensure the primers for each cluster group are exclusive.

Results
Two approaches were applied to improve primer design and re-cluster fish species. The results have shown that exclusive primers for 2618 fish species were successfully discovered through in silico analysis. In addition, we applied sequence alignment analysis to confirm that each pair of primers can successfully identify all collected fish species at the taxonomic family levels.

Conclusions
This study provided a practical strategy to discover unique primers for each fishery species and a comprehensive list of exclusive primers for extracting COI barcode sequences of all known fishery species. Various applications of verification of fish products or identification of unknown fish species could be effectively achieved.
RSAP-Net: joint optic disc and cup segmentation with a residual spatial attention path module and MSRCR-PT pre-processing algorithm
Authors: Jiang Yun , Ma Zeqi , Wu Chao , Zhang Zequn , Yan Wei 
Published: December 2022

AbstractBackgroundGlaucoma can cause irreversible blindness to people’s eyesight. Since there are no symptoms in its early stage, it is particularly important to accurately segment the optic disc (OD) and optic cup (OC) from fundus medical images for the screening and prevention of glaucoma. In recent years, the mainstream method of OD and OC segmentation is convolution neural network (CNN). However, most existing CNN methods segment OD and OC separately and ignore the a priori information that OC is always contained inside the OD region, which makes the segmentation accuracy of most methods not high enough.MethodsThis paper proposes a new encoder–decoder segmentation structure, called RSAP-Net, for joint segmentation of OD and OC. We first designed an efficient U-shaped segmentation network as the backbone. Considering the spatial overlap relationship between OD and OC, a new Residual spatial attention path is proposed to connect the encoder–decoder to retain more characteristic information. In order to further improve the segmentation performance, a pre-processing method called MSRCR-PT (Multi-Scale Retinex Colour Recovery and Polar Transformation) has been devised. It incorporates a multi-scale Retinex colour recovery algorithm and a polar coordinate transformation, which can help RSAP-Net to produce more refined boundaries of the optic disc and the optic cup.ResultsThe experimental results show that our method achieves excellent segmentation performance on the Drishti-GS1 standard dataset. In the OD and OC segmentation effects, the F1 scores are 0.9752 and 0.9012, respectively. The BLE are 6.33 pixels and 11.97 pixels, respectively.ConclusionsThis paper presents a new framework for the joint segmentation of optic discs and optic cups, called RSAP-Net. The framework mainly consists of a U-shaped segmentation skeleton and a residual space attention path module. The design of a pre-processing method called MSRCR-PT for the OD/OC segmentation task can improve segmentation performance. The method was evaluated on the publicly available Drishti-GS1 standard dataset and proved to be effective.
Identification of multiplicatively acting modulatory mutational signatures in cancer
Authors: Kičiatovas Dovydas , Guo Qingli , Kailas Miika , Pesonen Henri , Corander Jukka , Kaski Samuel , Pitkänen Esa , Mustonen Ville 
Published: December 2022

Abstract
Background
A deep understanding of carcinogenesis at the DNA level underpins many advances in cancer prevention and treatment. Mutational signatures provide a breakthrough conceptualisation, as well as an analysis framework, that can be used to build such understanding. They capture somatic mutation patterns and at best identify their causes. Most studies in this context have focused on an inherently additive analysis, e.g. by non-negative matrix factorization, where the mutations within a cancer sample are explained by a linear combination of independent mutational signatures. However, other recent studies show that the mutational signatures exhibit non-additive interactions.

Results
We carefully analysed such additive model fits from the PCAWG study cataloguing mutational signatures as well as their activities across thousands of cancers. Our analysis identified systematic and non-random structure of residuals that is left unexplained by the additive model. We used hierarchical clustering to identify cancer subsets with similar residual profiles to show that both systematic mutation count overestimation and underestimation take place. We propose an extension to the additive mutational signature model—multiplicatively acting modulatory processes—and develop a maximum-likelihood framework to identify such modulatory mutational signatures. The augmented model is expressive enough to almost fully remove the observed systematic residual patterns.

Conclusion
We suggest the modulatory processes biologically relate to sample specific DNA repair propensities with cancer or tissue type specific profiles. Overall, our results identify an interesting direction where to expand signature analysis.
Comparative transcriptome analysis reveals differences in gene expression in whitefly following individual or combined applications of Akanthomyces attenuatus (Zare & Gams) and matrine
Authors: Wu Jianhui , Sun Tingfei , Bashir Muhammad Hamid , Qiu Baoli , Wang Xingmin , Ali Shaukat 
Published: December 2022

Abstract
Background
Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) is a serious pest of crops in different regions of the world. Our recent studies on the joint application of Akanthomyces attenuatus (a pathogenic insect fungus) and matrine (a botanical insecticide) against B. tabaci have shown promising results. Using RNA sequencing (RNA-Seq), we identified differentially expressed genes involved in whitefly responses to single or mixed applications of A. attenuatus and matrine.

Methods
In this study, we compared the transcriptome profiles of B. tabaci treated with individual and combined treatments of A. attenuatus and matrine to determine variations in gene expression among whiteflies in response to different treatments.

Results
Transcriptomic data analysis showed differential expression of 71, 1194, and 51 genes in response to A. attenuatus (BtA), matrine (BtM), and A. attenuatus + matrine (BtAM) treatment, respectively. A total of 65 common differentially expressed genes (DEGs) were identified between whiteflies treated with A. attenuatus (BtA) and matrine (BtM). A comparison of DEGs across the three treatments (BtA, BtM, and BtAM) revealed two common DEGs. The results also revealed that AMPK signaling, apoptosis, and drug metabolism pathways are likely involved in whitefly defense responses against A. attenuatus and matrine infection. Furthermore, a notable suppression of general metabolism and immune response genes was observed in whiteflies treated with A. attenuatus + matrine (BtAM) compared to whiteflies treated with individual A. attenuatus (BtA) or matrine (BtM) treatments.

Conclusion
Dynamic changes in the number of differentially expressed genes were observed in B. tabaci subjected to different treatments (BtA, BtM, and BtAM). To the best of our knowledge, this is the first report on the molecular interactions between whitefly and individual or combined treatments of A. attenuatus and matrine. These results will further improve our knowledge of the infection mechanism and complex biochemical processes involved in the synergistic action of A. attenuatus and matrine against B. tabaci.