bluuebunny/crossref_metadata_2025_split huggingface dataset:
Automated soccer head impact exposure tracking using video and deep learning
Authors: Rezaei Ahmad , Wu Lyndia C. 
Published: June 2022

AbstractHead impacts are highly prevalent in sports and there is a pressing need to investigate the potential link between head impact exposure and brain injury risk. Wearable impact sensors and manual video analysis have been utilized to collect impact exposure data. However, wearable sensors suffer from high deployment cost and limited accuracy, while manual video analysis is a long and resource-intensive task. Here we develop and apply DeepImpact, a computer vision algorithm to automatically detect soccer headers using soccer game videos. Our data-driven pipeline uses two deep learning networks including an object detection algorithm and temporal shift module to extract visual and temporal features of video segments and classify the segments as header or nonheader events. The networks were trained and validated using a large-scale professional-level soccer video dataset, with labeled ground truth header events. The algorithm achieved 95.3% sensitivity and 96.0% precision in cross-validation, and 92.9% sensitivity and 21.1% precision in an independent test that included videos of five professional soccer games. Video segments identified as headers in the test data set correspond to 3.5 min of total film time, which can be reviewed through additional manual video verification to eliminate false positives. DeepImpact streamlines the process of manual video analysis and can help to collect large-scale soccer head impact exposure datasets for brain injury research. The fully video-based solution is a low-cost alternative for head impact exposure monitoring and may also be expanded to other sports in future work.
RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation
Authors: Guénolé Aude , Velilla Fabien , Chartier Aymeric , Rich April , Carvunis Anne-Ruxandra , Sardet Claude , Simonelig Martine , Sobhian Bijan 
Published: June 2022

AbstractPost-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization. Here, we show that the RING finger protein RNF219 co-purifies with the CCR4-NOT complex, the major mRNA deadenylase in eukaryotes, which mediates translational repression in both a deadenylase activity-dependent and -independent manner. Strikingly, RNF219 both inhibits the deadenylase activity of CCR4-NOT and enhances its capacity to repress translation of a target mRNA. We propose that the interaction of RNF219 with the CCR4-NOT complex directs the translational repressive activity of CCR4-NOT to a deadenylation-independent mechanism.
A simple cut and stretch assay to detect antimicrobial resistance genes on bacterial plasmids by single-molecule fluorescence microscopy
Authors: Goyal Gaurav , Ekedahl Elina , Nyblom My , Krog Jens , Fröbrant Erik , Brander Magnus , Sewunet Tsegaye , Tangkoskul Teerawit , Giske Christian G. , Sandegren Linus , Thamlikitkul Visanu , Ambjörnsson Tobias , Westerlund Fredrik 
Published: June 2022

AbstractAntimicrobial resistance (AMR) is a fast-growing threat to global health. The genes conferring AMR to bacteria are often located on plasmids, circular extrachromosomal DNA molecules that can be transferred between bacterial strains and species. Therefore, effective methods to characterize bacterial plasmids and detect the presence of resistance genes can assist in managing AMR, for example, during outbreaks in hospitals. However, existing methods for plasmid analysis either provide limited information or are expensive and challenging to implement in low-resource settings. Herein, we present a simple assay based on CRISPR/Cas9 excision and DNA combing to detect antimicrobial resistance genes on bacterial plasmids. Cas9 recognizes the gene of interest and makes a double-stranded DNA cut, causing the circular plasmid to linearize. The change in plasmid configuration from circular to linear, and hence the presence of the AMR gene, is detected by stretching the plasmids on a glass surface and visualizing by fluorescence microscopy. This single-molecule imaging based assay is inexpensive, fast, and in addition to detecting the presence of AMR genes, it provides detailed information on the number and size of plasmids in the sample. We demonstrate the detection of several β-lactamase-encoding genes on plasmids isolated from clinical samples. Furthermore, we demonstrate that the assay can be performed using standard microbiology and clinical laboratory equipment, making it suitable for low-resource settings.
Premorbid cancer and motor reserve in patients with Parkinson’s disease
Authors: Oh Yoon-Sang , Yoo Sang-Won , Lyoo Chul Hyoung , Lee Kwang-Soo , Kim Joong-Seok 
Published: June 2022

AbstractDecreased cancer risk has been reported in patients with Parkinson’s disease (PD), and cancer prior to PD can have a protective effect on PD risk. We investigated cancer history prior to PD diagnosis to determine if such history can enhance motor reserve in PD by assessing the association between motor deficits and striatal subregional dopamine depletion. A total of 428 newly diagnosed, drug-naïve PD patients was included in the study. PD patients were categorized into three groups of no prior neoplasia, premorbid precancerous condition, and premorbid malignant cancer before PD diagnosis. Parkinsonian motor status was assessed using the Unified Parkinson’s Disease Rating Scale (UPDRS) motor score and modified Hoehn and Yahr stage score. All patients underwent positron emission tomography (PET) with 18F-N-(3-fluoropropyl)-2beta-carbon ethoxy-3beta-(4-iodophenyl) nortropane (18F-FP-CIT), and the regional standardized uptake value ratios (SUVRs) were analyzed with a volume-of-interest template among the groups. The UPDRS motor score negatively correlated with SUVRs in the posterior putamen for all patient groups. Groups with neoplasia, especially those with premorbid cancer, showed lower motor scores despite similar levels of dopamine depletion in the posterior putamen relative to those without neoplasia. These results suggest that premorbid cancer acts as a surrogate for motor reserve in patients with PD and provide imaging evidence that history of cancer has a protective effect on PD.
Prognostic implication of IgG4 and IgG1-positive cell infiltration in the lung in patients with idiopathic interstitial pneumonia
Authors: Komatsu Masamichi , Yamamoto Hiroshi , Uehara Takeshi , Kobayashi Yukihiro , Hozumi Hironao , Fujisawa Tomoyuki , Miyamoto Atsushi , Kishaba Tomoo , Kunishima Fumihito , Okamoto Masaki , Kitamura Hideya , Iwasawa Tae , Matsushita Shoichiro , Terasaki Yasuhiro , Kunugi Shinobu , Ushiki Atsuhito , Yasuo Masanori , Suda Takafumi , Hanaoka Masayuki 
Published: June 2022

AbstractImmunoglobulin (Ig) G4-positive cells are rarely observed in the lungs of patients with idiopathic interstitial pneumonias (IIPs). IgG1 may be more pathogenic than IgG4, with IgG4 having both pathogenic and protective roles in IgG4-related disease (IgG4-RD). However, the role of both IgG1 and IgG4 in IIPs remains unclear. We hypothesized that patients with IgG4-positive interstitial pneumonia manifest different clinical characteristics than patients with IgG4-RD. Herein, we identified the correlation of the degree of infiltration of IgG1- and IgG4-positive cells with IIP prognosis, using a Japanese nationwide cloud-based database. We included eighty-eight patients diagnosed with IIPs after multidisciplinary discussion, from April 2009 to March 2014. IgG4-positive cell infiltration was identified in 12/88 patients with IIPs and 8/41 patients with idiopathic pulmonary fibrosis (IPF). Additionally, 31/88 patients with IIPs and 19/41 patients with IPF were diagnosed as having IgG1-positive cell infiltration. IgG4-positive IIPs tended to have a better prognosis. Conversely, overall survival in cases with IgG1-positive IPF was significantly worse. IIPs were prevalent with IgG1- or IgG4-positive cell infiltration. IgG1-positive cell infiltration in IPF significantly correlated with a worse prognosis. Overall, evaluating the degree of IgG1-positive cell infiltration may be prognostically useful in cases of IPF.
Genetic structure of Sclerotinia sclerotiorum populations from sunflower and cabbage in West Azarbaijan province of Iran
Authors: Faraghati Masoumeh , Abrinbana Masoud , Ghosta Youbert 
Published: June 2022

AbstractSclerotinia sclerotiorum is one of the most destructive fungal pathogens infecting a wide array of plant species worldwide. Management of this pathogen relies on the coordinated use of fungicides and resistant host cultivars with other control measures, but the effectiveness of these methods requires knowledge of the genetic variability and structure of the fungal populations. To provide insight into the genetic diversity and structure of this pathogen in West Azarbaijan province of Iran, a total of 136 isolates were collected from symptomatic sunflower and cabbage plants within fields in three regions and analysed using inter-simple sequence repeat (ISSR) markers and intergenic spacer (IGS) region of the rRNA gene sequences. A total of 83 ISSR multilocus genotypes (MLGs) were identified, some of which were shared among at least two regional or host populations but in a low frequency. High genotypic diversity, low levels of clonal fraction, and random association of ISSR loci in a region indicated a low level of clonal reproduction, and possibly a high level of sexually recombining life cycle for the pathogen in the province. Marker analyses revealed that the pathogen was spatially homogeneous among fields, and thus similar control measures, such as the choice of resistant cultivars and fungicides, may effectively manage S. sclerotiorum within the region. Four IGS haplotypes (IGS1–IGS4) were detected within populations with IGS3 being the most prevalent haplotype. The low IGS haplotype diversity, the absence of spatial structure, and shared MLGs among populations may suggest a single introduction and subsequent dispersal of S. sclerotiorum within West Azarbaijan province.
Experimental and simulation study on heat transfer characteristics of aluminium alloy piston under transition conditions
Authors: Liu Yang , Lei Jilin , Wang Dongfang , Deng Xiwen , Wen Jun , Wen Zhigao 
Published: June 2022

AbstractIn order to explore the thermal load change of the diesel engine piston under transitional conditions, and the influence of the position of cooling gallery on the heat transfer characteristics of the piston. An off-road high-pressure common-rail diesel engine is chosen as the research object. The sequence coupling method is used to establish the fluid–solid coupling heat transfer simulation model of the piston-gallery under the transition conditions of cold start, urgent acceleration and rapid deceleration. The Pareto optimization algorithm is introduced to optimize the position of the cooling gallery to reduce the maximum temperature and maximum thermal stress of the piston. The results show that the maximum temperature of the piston can be reduced by reducing the distance between the cooling gallery and the throat area under the maximum torque condition, and that the maximum thermal stress of the piston can be reduced by reducing the distance between the cooling gallery and the throat area or by increasing the distance between the cooling gallery and the ring area. Compared with the original design, the maximum temperature of Design A decreases by 1.28 °C while the maximum thermal stress decreases by 2.07 MPa. The maximum temperature and maximum thermal stress of Design B decreases by 0.22 °C and 0.5 MPa, respectively. The maximum thermal stress of Design C decreases by 2.67 MPa when the maximum temperature increases by 1.15 °C. The maximum change in temperature of the three typical designs and the original design of the piston throat under cold start, urgent acceleration and rapid deceleration conditions reached 207.29 °C, 136.78 °C and 9.89 °C, and the maximum change of thermal stress reached 8.62 MPa, 20.43 MPa, 4.08 MPa, respectively. The maximum change in temperature of the piston first ring groove under cold start, urgent acceleration and rapid deceleration conditions reached 172.00 °C, 83.52 °C and 7.36 °C, and the maximum change in thermal stress reached 22.96 MPa, 43.10 MPa, 5.72 MPa, respectively. The conclusions obtained can provide boundary conditions for further study of the thermal load change law of the same type of pistons, and also provide a theoretical basis for diesel engine piston structure optimization and the performance improvement.
Potential anti-hepatocellular carcinoma properties and mechanisms of action of clerodane diterpenes isolated from Polyalthia longifolia seeds
Authors: Tatipamula Vinay Bharadwaj , Thonangi Chandi Vishala , Dakal Tikam Chand , Vedula Girija Sastry , Dhabhai Bhanupriya , Polimati Haritha , Akula Annapurna , Nguyen Ha Thi 
Published: June 2022

AbstractDiterpenes are secondary metabolites that have attracted much attention due to their potential biological activities including anti-cancer potential. The aim of the current study is to assess the anticancer potential of the six known clerodane diterpenes (1–6) isolated fromPolyalthia longifoliaseeds and their underlying molecular mechanisms. These compounds were evaluated for their cytotoxicity in vitro by using MTT assays. The “two-phase model” with NDEA and PB ad libitum was used for induction of HCC and sorafenib was used as the standard drug. Prophylactic studies were carried out for compounds4/6at both low (5 mg/kg b.w) and high (10 mg/kg b.w) doses. Based on the MTT assay results, the two best compounds,4and6, were selected for in vivo studies. The results showed that treatment with compound4/6significantly restored the changes in biochemical parameters and liver morphology observed in (NDEA + PB)-induced HCC rats. Additionally, the docking studies showed that compound4/6interacted with several key proteins such as MDM2, TNF-α, FAK, thereby inhibiting these proteins and reversing the negative impacts of NDEA. In conclusion, our results suggested that compounds4and6are potential therapeutic agents for HCC, mostly due to their ability to control typical cancer pathways.
5 days of time-restricted feeding increases fat oxidation rate but not affect postprandial lipemia: a crossover trial
Authors: Chiu Chih-Hui , Chen Che-Hsiu , Wu Min-Huan , Lan Pei-Tzu , Hsieh Yu-Chen , Lin Zong-Yan , Chen Bo-Wei 
Published: June 2022

AbstractStudies have revealed that time-restricted feeding affects the fat oxidation rate; however, its effects on the fat oxidation rate and hyperlipidemia following high-fat meals are unclear. This study investigated the effects of 5-day time-restricted feeding on the fat oxidation rate and postprandial lipemia following high fat meals. In this random crossover experimental study, eight healthy male adults were included each in the 5-day time-restricted feeding trial and the control trial. The meals of the time-restricted feeding trial were provided at 12:00, 16:00, and 20:00. The meals of the control trial were provided at 08:00, 14:00, and 20:00. The contents of the meals of both trials were the same, and the calories of the meals met the 24-h energy requirement of the participants. After 5 days of the intervention, the participants consumed high-fat meals on the sixth day, and their physiological changes were determined. The fasting fat oxidation rate (p < 0.001) and postprandial fat oxidation rate (p = 0.019) of the time-restricted feeding trial were significantly higher than those of the control trial. The 24-h energy consumption and postprandial triglyceride, blood glucose, insulin, glycerol, and free fatty acid concentrations of the two trials showed no significant differences (p > 0.05). The results revealed that 5 days of time-restricted feeding effectively increased the fasting and postprandial fat oxidation rate, but it did not affect postprandial lipemia.
Characterization of an aerosolized nanoparticle beam beyond the diffraction limit through strong field ionization
Authors: Davino Michael , Saule Tobias , Helming Nora G. , Powell J. A. , Trallero-Herrero Carlos 
Published: June 2022

AbstractThe study of nanomaterials is an active area of research for technological applications as well as fundamental science. A common method for studying properties of isolated nanoparticles is by an in-vacuum particle beam produced via an aerodynamic lens. Despite being common practice, characterization of such beams has proven difficult as light scattering detection techniques fail for particles with sizes beyond the diffraction limit. Here we present a new technique for characterizing such nanoparticle beams using strong field ionization. By focusing an ultrafast, mJ-level laser into the particle beam, a nanoparticle within the laser focus is ionized and easily detected by its ejected electrons. This method grants direct access to the nanoparticle density at the location of the focus, and by scanning the focus through the transverse and longitudinal profiles of the particle beam we attain the 3-dimensional particle density distribution for a cylindrically symmetric beam. Further, we show that strong field ionization is effective in detecting spherical nanoparticles as small as 10 nm in diameter. Additionally, this technique is an effective tool in optimizing the particle beam for specific applications. As an example we show that the particle beam density and width can be manipulated by restricting the gas flow into the aerodynamic lens.
Evaluating diagnostic accuracy and determining optimal diagnostic thresholds of different approaches to [68Ga]-DOTATATE PET/MRI analysis in patients with meningioma
Authors: Kim Sean H. , Roytman Michelle , Madera Gabriela , Magge Rajiv S. , Liechty Benjamin , Ramakrishna Rohan , Pannullo Susan C. , Schwartz Theodore H. , Karakatsanis Nicolas A. , Osborne Joseph R. , Lin Eaton , Knisely Jonathan P. S. , Ivanidze Jana 
Published: June 2022

AbstractMultiple approaches with [68Ga]-DOTATATE, a somatostatin analog PET radiotracer, have demonstrated clinical utility in evaluation of meningioma but have not been compared directly. Our purpose was to compare diagnostic performance of different approaches to quantitative brain [68Ga]-DOTATATE PET/MRI analysis in patients with suspected meningioma recurrence and to establish the optimal diagnostic threshold for each method. Patients with suspected meningioma were imaged prospectively with [68Ga]-DOTATATE brain PET/MRI. Lesions were classified as meningiomas and post-treatment change (PTC), using follow-up pathology and MRI as reference standard. Lesions were reclassified using the following methods: absolute maximum SUV threshold (SUV), SUV ratio (SUVR) to superior sagittal sinus (SSS) (SUVRsss), SUVR to the pituitary gland (SUVRpit), and SUVR to the normal brain parenchyma (SUVRnorm). Diagnostic performance of the four methods was compared using contingency tables and McNemar’s test. Previously published pre-determined thresholds were assessed where applicable. The optimal thresholds for each method were identified using Youden’s J statistics. 166 meningiomas and 41 PTC lesions were identified across 62 patients. SUV, SUVRsss, SUVRpit, and SUVRnorm of meningioma were significantly higher than those of PTC (P < 0.0001). The optimal thresholds for SUV, SUVRsss, SUVRpit, and SUVRnorm were 4.7, 3.2, 0.3, and 62.6, respectively. At the optimal thresholds, SUV had the highest specificity (97.6%) and SUVRsss had the highest sensitivity (86.1%). An ROC analysis of SUV, SUVRsss, SUVRpit, and SUVRnorm revealed AUC of 0.932, 0.910, 0.915, and 0.800, respectively (P < 0.0001). Developing a diagnostic threshold is key to wider clinical translation of [68Ga]-DOTATATE PET/MRI in meningioma evaluation. We found that the SUVRsss method may have the most robust combination of sensitivity and specificity in the diagnosis of meningioma in the post-treatment setting, with the optimal threshold of 3.2. Future studies validating our findings in different patient populations are needed to continue optimizing the diagnostic performance of [68Ga]-DOTATATE PET/MRI in meningioma patients.Trial registration: ClinicalTrials.gov Identifier: NCT04081701. Registered 9 September 2019. https://clinicaltrials.gov/ct2/show/NCT04081701.
Activation of the TGF-β1/Smads/α-SMA pathway is related to histological and functional changes in children with neurogenic bladder
Authors: Yang Xinghuan , Pu Qingsong , Wen Yibo , Zhao Yi , Wang Junkui , Xu Pengchao , Ma Yuan , Liu Erpeng , Lv Lei , Wen Jian Guo 
Published: June 2022

AbstractThis research is to investigate the expression of the TGF-β1/Smads/α-SMA pathway and its effect on bladder histology and function in children with neurogenic bladder (NB). The bladder specimens from 10 children with NB and 8 children with vesicoureteral junction obstruction were collected into the NB and control groups. The expression of TGF-β1, Smad2, Smad3, Smad4, Smad6, α-SMA, fibronectin, collagen I and collagen III in bladder tissues was detected. In addition, the histological characteristics of the bladder were evaluated. A preoperative urodynamic study was performed on all children with NB. We analysed the correlations among the expression of the marker protein a-SMA in myofibroblasts, effector cells of the pathway, and bladder function parameters. Compared with those in the control group, the expression of TGF-β1, Smad2, Smad3, Smad4, α-SMA, fibronectin, collagen I and collagen III was significantly increased in the NB group, while the expression of Smad6 was decreased (p < 0.01). HE and Masson staining in the NB group showed increased collagen levels and hypertrophy of smooth muscle cells. Children with NB had a low bladder volume ratio (BVR), low compliance (△C) and high maximum bladder pressure, low maximum flow rate, large postvoid residual volume, low bladder contraction index and low bladder voiding efficiency. The expression of α-SMA was negatively correlated with the BVR (r =  − 0.7066, P = 0.0223) and △C (r =  − 0.6516, P = 0.0412). We conclude that the TGF-β1/Smads/α-SMA pathway is activated in the bladder tissue of children with NB and may be involved in the processes causing histological and functional changes.
Reliable fluorescence technique to detect the antibiotic colistin, a possible environmental threat due to its overuse
Authors: Mandal Saurodeep , Dey Bhowmik Arpan , Mukhuty Alpana , Kundu Shampa , Truong Khai-Nghi , Rissanen Kari , Chattopadhyay Ansuman , Sahoo Prithidipa 
Published: June 2022

AbstractColistin, considered a drug of last resort as it is effective towards multidrug-resistant Gram-negative bacterial infections. Oral administration of colistin in the poultry industry is a common practice, not only to prevent and reduce bacterial infections, but also as a rapid-growth promoter. Long-term exposure to any antibiotic will eventually lead to the development of bacterial resistance towards all antibiotics through various mechanisms in the physiological system and environment. Chicken is the most consumed source of animal protein for humans throughout the world. In addition, the manure of poultry, containing traces of the used antibiotics, is being used in farming. Exposure to excess amounts of colistin causes a great concern not only to the humans but to the environment as a whole. In the present contribution, colistin has been detected in chicken hepatocyte cells through in vivo confocal microscopy. In addition, the amount of colistin in the chicken excrements has been estimated. A simple chemosensor NAF, a dye-based on napthaldehyde furfural, was developed for the detection of colistin, supplemented with experimental evidence and theoretical calculations.