bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRODUCTION OF POLYCLONAL ANTIBODY AGAINST THE OUTER MEMBRANE PROTEIN Omp48 OF Aeromonas hydrophila
Authors: Thi Hoa Rol , Nguyen Van Hau , Do Nguyen Trong Tri , Dang Thi Phuong Thao , Nguyen Trinh 
Published: May 2022

Aeromonas hydrophila is one of the main pathogens in fish, causing a huge economic loss every year. Since outer membrane protein Omp48 had been demonstrated to be immunogenic in fish, it has gained attention as a potential vaccine candidate to protect fish from A. hydrophila. In this study, we produced a polyclonal antibody against Omp48, which would be necessary for further studies on this protein. To reach this aim, 6xHis-tagged Omp48 was expressed in a recombinant E. coli strain and purified by a nickel affinity chromatography column, which yielded a purity of 97.3 ± 1.4%. The purified Omp48 was injected into rabbits in order to produce polyclonal anti-Omp48 antibody. Subsequently, we analyzed the obtained antiserum, which was demonstrated to have high specificity and sensitivity to the Omp48 protein by Western blot and ELISA, with the titer of 1:1,280,000. In addition, we also showed that the antiserum could be used to detect Omp48 in immunofluorescence staining.
Code Switching Found in Daniel Mananta’s Utterances on YouTube Channel
Authors: Petrus Stefanus Pagul  , I Gusti Agung Sri Rwa Jayantini  , Ni Nyoman Deni Ariyaningsih  
Published: May 2022

This study concerns with code-switching in Daniel Mananta’s utterance. This study aims to answer the research problems, which are: (1) to classify the types of code switching found in Daniel Mananta’s utterance and (2) and to analyze the function of code switching. Descriptive qualitative method was applied to identify the data. Code-switching was described according to a theory proposed by Poplack (1980) to classify the types of code-switching and the theory by Apple& Muysken (1987) to describe the function of code switching found in Daniel Mananta’s utterance. The result of this study shows that all types of code switching and six function of code-switching were found. First, the three types of code-switching were found, namely tag switching, intra-sentential switching, and inter-sentential switching, in which the most dominant switching is intra-sentential switching. It occurs because Daniel Mananta looks more comfortable when using words, clauses, and phrases in one sentence to convey motivational messages. Second, six functions of code-switching were found, namely referential function, directive function, expressive function, phatic function, metalinguistic function, poetic function. The most dominant function is phatic function because Daniel Mananta’s feels free and more comfortable expressing his emotional feelings in a language that is used in his daily language.
KAJIAN TINGKAT PARTISIPASI, TRANSPARANSI DAN AKUNTABILITAS PENGELOLAAN DANA OTONOMI KHUSUS PAPUA BERDASARKAN TIPOLOGI WILAYAH
Authors: Iek Mesak 
Published: May 2022

Objective of the Study is to identify planning and financial management problem which are sourced from Papua's Special Autonomy Fund for education and health services in mountainous areas, difficult access areas and easy access areas. The approach used in this study is a case study which is one of the qualitative research approaches using inductive logic process. The scope of the study area is divided into three regions, namely easy access plains, coastal lowlands and difficult access, and mountains. Samples were taken according to the typology of district areas, namely: (a) the mountainous region : Jayawijaya, Tolikara, and Mountains, (b) difficult access of coastal areas: Asmat regency, Yapen Islands, Keerom, (c) easy access plains areas : District Supiori, Merauke and Jayapura. Furthermore, 1 district and 2 villages were taken from each district. The samples were taken from each district in accordance with the studies that may represent the locus of social organization, executives and legislative those are SKPD and hospitals. While for the sample of villages/urban population were taken representatively from elementary school, junior high school and Health Center. And for the study of individual locus that represent the population are teachers, doctors, health workers, traditional leaders, religious leaders, and households are all Papuans so that the sample in this study amounted to 598 people. In this case, statistical descriptive analysis tool is used. This study found that the Management which is not optimal in 3 areas are Reporting and Internal Control. Activities funded by the special autonomy fund did not inform freely to the public. During this time the local government did not involve teachers, medical personnel and Papua native communities to do the planning, implementation and monitoring of spending special autonomy funds. During these activities are carried out by SKPD which is financed by the special autonomy funds are not openly communicated to all those in need: easy access areas, difficult access areas, and mountainous areas. At the planning stage, programs and activities funded by Autonomy funds are not transparent. The only real activity that is known by society is Respek (villages’ strategic planning development). Many activities where the source of activity and funds are still unknown by teachers, medical personnel, and the public. According to the society (sample), the management of special autonomy funds is not accountable. The local government is necessary to disseminate the activities that use special autonomy funds, as well as formal and informal information to the public about the activities that used special autonomy funds. All stakeholders need to be involved to participate in the management system of special autonomy fund. Musrembang format that has been carried out needs to be done properly.Keyword : Participation, Transparency, Accountability, Special Autonomy Funds
ANTROPOLOGI EKONOMI : MASYARAKAT MEYBRAT DI KEPALA BURUNG PAPUA BARAT
Authors: Kambu Arius A. 
Published: May 2022

The purpose of this study is to explain in depth and thorough about the value of kinship and consumptive pattern that gives a clear impact on the pattern of the business establishment of Meybrat people. The sample access is all kinds of Meybrat people’s business. This study used a qualitative analysis of a single case study approach. The results of this study found that (1) the business of Meybrat people is still at the stage of looking for shapes (innovation , triggering) where the personal character (personal values, education, experience, risk taking, internal control) are still weak in shaping the behavior of Meybrat people’s business; (2) business groups (micro, small and medium enterprises) are more successful and grow well on an individual level than on groups; (3) Factors of kinship consumptive pattern systems and communities include: payment of dowry, families and relatives’ education costs which is still a strong influence in shaping the conduct of business of Meybrat people.Keywords: Kinship, Consumer Pattern, Productive Economy
Pengaruh Alokasi Anggaran Pemerintah Terhadap Aktivitas Pemberdayaan Perempuan Di Kota Jayapura
Authors: Normayunita Normayunita , Mollet Julius Ary , Bonay Antonia Klara 
Published: May 2022

Tujuan dari penelitian ini adalah untuk mengetahui berapa banyak alokasi anggaran yang tersalurkan ke kaum perempuan dari program-program kegiatan yang dibuat oleh Badan Pemberdayaan Perempuan yang ada di Kota Jayapura. Penelitian dilakukan dengan metode kasus dengan mengkombinasikan bebagai cara dalam pengambilan data untuk membahas topik diatas secara deskriptif kualitatif, dengan analisis data menggunakan analisis SWOT yaitu metode perencanaan strategis yang digunakan untuk mengevaluasi kekuatan (strengths), kelemahan (weaknesses), peluang (opportunities), dan ancaman (threats) dalam suatu obyek penelitian. Kata Kunci: Alokasi Anggaran, Aktivitas, Pemberdayaan Perempuan
Pengembangan Pertanian Padi Sawah Masyarakat Suku Marind Di Kabupaten Merauke
Authors: Mollet Julius Ary 
Published: May 2022

Kajian ini merupakan hasil laporan penelitian yang didanai oleh Penelitain Prioritas Nasional MP3EI Tahun 2015 Tahap ke II. Tujuan penelitian : 1) untuk mengkaji pola pengelolaan tanaman padi suku Marind, 2) menganalisa tingkat keuntungan produksi tanaman padi masyarakat Marind. Dengan menggunakan simple random sampling, total responden sebanyak 168 orang yang tersebar di tiga distirik (Kurik, Semanggi dan Tanah Miring). Pendekatan kuantitatif dan kualitatif digunakan dalam penelitian ini. Hasil penelitan memperlihatkan bahwa terjadi perubahan sistem pertanian suku Marid dari subsisten ke mekanisasi pertanian, serta masyarkat Marind mampu mengembangkan tanaman padi. Selanjuntya hasil analisa BEP, memperlihatkan masyarakat Maridn masih memperopleh keuntungan dalam memproduksi padi.Kata Kunci : Pengembangan Pertanian, Tanaman Padi, Suku Marind
Analisis Produktivitas Komoditi Kelapa Kabupaten Sarmi
Authors: Ngutra Risky Novan , Kakisina Charlota Stella 
Published: May 2022

Tanaman Kelapa (Coco nucifera L) merupakan salah satu tanaman asli Indonesia yang sangat potensial sebagai komoditas perdagangan baik dalam negeri maupun luar negeri. Masyarakat pada umumnya, sudah sejak lama mengenal tanaman kelapa sebagai tanaman multifungsi yang dapat dimanfaatkan hampir semua bagiannya. Kabupaten Sarmi termasuk kabupaten penghasil kelapa terbesar di Provinsi Papua dengan luas wilayah tanam produksi kelapa terbesar di Papua. Selain itu, jika dilihat dari jenis tanaman perkebunan, komoditas kelapa di Kabupaten Sarmi termasuk komoditas unggulan. Penelitian ini menganisis“Bagaimana pengembangan produktivitas komoditi kelapa kabupaten Sarmi; serta bagaimana arah kebijakan pengembangan komoditas kelapa untuk memfasilitasi pertumbuhan ekonomi di Kabupaten Sarmi. Hasil penelitian ini memperlihatkan bahwa penerimaan dari usaha tani kelapa secara monokultur jika dihitung berdasarkan harga yang berlaku dengan luas lahan yang dimiliki/dikelola, diperoleh penerimaan sebesar Rp 2.700.000/ha/tahun sampai dengan Rp. 3.100.000/ha/tahun atau Rp.225.000/ha/bulan sampai dengan 258.000/ha/bulan. Sedangkan penerimaan yang diterima oleh petani kelapa di kabupaten setelah di hitung menghasilkan penerimaan adalah dengan usahatani kopra sebesar Rp. 36,971,650,000,-, dengan usahatani buah kelapa sebesar Rp 22,182,350,000.- dan usahatani minyak kelapa sebesar Rp 9,981,470,000,-. Sedangkan arah kebijakan yang dapat meningkatkan nilai kelapa untuk mendukung perekonomian petani perlu dilakukan pengembangan komoditas kelapa yang diarahkan pada peningkatan produktivitas melalui penggunaan bibit unggul dan pengelolaan usaha tani yang efisien, pengembangan produk kelapa yang bernilai ekonomi dengan mutu yang sesuai permintaan pasar, pemberdayaan kelompok tani atau gapoktan yang bermitra dengan industri kelapa/eksportir, bantuan teknis pembinaan dan pembiayaan bagi gapoktan dari instansi terkait yang terprogram dan berkelanjutan.Kata Kunci: Produktivitas, Komoditi Kelapa, Pertumbuhan Ekonomi
Analisis Penerimaan Retribusi Pelayanan Parkir Di Tepi Jalan Umum Berdasarkan Perda Kota Jayapura Nomor 14 Tahun 2007
Authors: Saragi Rosari Uli Artha , Siahainenia Joddy E. H. , Indahyani Rachmaeny 
Published: May 2022

Penelitian ini bertujuan untuk mengetahui bagaimana jumlah lahan parkir, jumlah kendaraan dan PDRB berpengaruh terhadap penerimaan RPPTJU, perkembangan penerimaan RPPTJU sebelum dan sesudah Perda No.14 tahun 2007 dan kontribusinya terhadap retribusi daerah. Objek yang diteliti adalah realisasi penerimaan RPPTJU, jumlah lahan parkir, jumlah kendaraan dan PDRB. Metode analisis data yang digunakan adalah regresi berganda, t-test dan kontribusi . Hasil analisis ini menunjukkan secara deskriptif kuantitatif ,lahan parkir dan kendaraan mempunyai pengaruh yang tidak signifikan dan PDRB sama sekali tidak berpengaruh, sedangkan Perkembangan sebelum dan sesudah Perda No.14 tahun 2007, secara umum dikatakan baik. Hal ini ditunjukkan dengan perkembangan realisasi RPPTJU sesudah berlakunya Perda, penerimaan RPPTJU meningkat dari 19,33 menjadi 20,5. Dan kontribusinya terhadap retribusi daerah pada tahun 2008 sebesar 5,59% selanjutnya terus mengalami penurunan sampai tahun 2011 menjadi 2,87%, pada tahun 2012 sempat mengalami kenaikan menjadi 4,45% dan kembali menurun pada tahun 2013 menjadi 3,75%.Kata Kunci: RPPTJU ,Jumlah Lahan Parkir, Jumlah Kendaraan, PDRB
Pushing the Boundaries of System Safety
Authors: Muniak Charles 
Published: May 2022

The 35th Annual ISSC was held August 21-25, 2017 in Albuquerque, New Mexico. This year’s theme was intended not only to redefine how system safety is applied in different domains, but to also reach into our past for lessons learned and go beyond our present thinking as we confront new challenges. It was intended to push the boundaries of the system of safety, as well as how we think about the safety of systems.
Highlights of the 35th International System Safety Conference
Letters to the Editor
Authors: Chelani Ashoka , Olayinka Fasusi 
Published: May 2022

“Safer than Safe Enough” – Australia leads the way?
The technical paper in the International System Safety Society’s (ISSS) Journal of System Safety Winter 2017 issue on “How Safe is Safe Enough? Acceptable Safety Criteria from an Engineering and Legal Perspective” by Martin Chizek is an interesting read, especially for Australian readers. This letter briefly talks about how Australian Workplace Health and Safety (WHS) legislation provides a framework for “Safer than Safe Enough” from a different engineering and legislative perspective.
Generating Sustainable Income for ISSS
I carefully read the points raised by Charles Hoes in his “TBD” column published on pages 6 and 7 of the Spring 2016 issue of Journal of System Safety. He raised some important issues concerning the future of the International System Safety Society (ISSS) and shared other thoughts regarding funding, vision and the relevance of the Society.
TBD
Authors: Hoes Charles 
Published: May 2022

My main focus during the Conference was on making sure that the Virtual Chapter’s suggestions for four Society initiatives received a fair hearing to determine whether to go forward with the ideas. The Executive Council and the people who attended our presentations were overwhelmingly in agreement that we brought good and valuable ideas that should be implemented by the International System Safety Society (ISSS) as a whole, rather than as independent actions of our small chapter. These four initiatives are:
1. Create a “motherhood” standard that describes the key goals and elements of a system safety program as understood and promoted by members of the ISSS.
2. Assist university engineering departments with integrating system safety concepts into their existing classes and curricula, starting with Arizona State University but expanding to other colleges and universities in the future.
3. Work to create system safety courses for system safety engineers that reflect the goals and elements of the “motherhood” standard developed in the first initiative.
4. Create a means of certifying system safety engineers as having the experience and expertise necessary to be fully qualified system safety engineers.
From the Editor's Desk
Authors: Muniak Charles 
Published: May 2022

I read a good number of scientific and technical journals, and almost every issue includes at least one article on some aspect of artificial intelligence (AI). The topics are usually about a new application, such as unmanned fighter jets, but quite often these articles touch upon the potential risks of these systems.
And, a letter to the editor by Rick Clarke points out that a JSS article by Nancy Leveson in our last issue (Vol. 55, No. 2), “Improving the Standard Risk Matrix using STPA,” may not be fully connected to the background of system safety.
System Safety in Healthcare
Authors: Raheja Dev , Escano Maria 
Published: May 2022

Unsafe work practices can happen in many ways. The following lengthy list includes examples of potential causes:

Excessive work for clinicians
Too many unnecessary reports and requirements
Over-dependence on technology
Conflict between the need for professional autonomy and establishing the dynamically changing best processes
Care delivery “silos” resulting from lack of interdepartmental teamwork
Constant distractions and interruptions
Too many policies and procedures, leading to a tendency to follow marginally effective methods
Over-reliance on electronic medical tracking taking precedence over bedside discussions with patients
Inattention to detail
Lack of motivation to get, or resources for, a second opinion
Quick diagnosis based on past observations
Inadequate attention to medical equipment dangers
Insufficient effort in infection prevention
People pretending the negative would not happen to them
Hospitals looking for quick profit
Questionable alternate boards certifying physicians who may not be qualified
A lack of passion for work
Unfavorable workflows, such as labs located far from the emergency department
A lack of clarity of what is required to assure patient safety
Too much team consensus instead of challenging the quality of intervention
Sex, Health and Australia’s Artarmon Triangle
Authors: Boulais Denis 
Published: May 2022

The Artarmon Triangle in Sydney, Australia is cornered by three large broadcast towers that, over the years, have emitted high levels of radiofrequency radiation. One study of the Artarmon Triangle suggests a link between the incidence of leukemia and proximity to the towers. Further, there is a widespread belief within the telecommunications industry that exposure to radiofrequency radiation may cause telecommunication equipment riggers to conceive more female offspring.
This study tests a hypothesis that telecommunications riggers who have worked on any of the three Artarmon Triangle towers would have an increased frequency of female offspring when compared to telecommunications riggers who have not worked in the Triangle.
Tips and Lessons Learned for Conducting Safety Review Board Meetings
Authors: Smith Robert 
Published: May 2022

At some point during a safety program’s lifecycle, presenting to an Independent Safety Review Board is likely. For the program representatives, including their safety lead, program manager, and supporting representatives (e.g., design engineers, software developers, test directors, etc.), this could be comparable to a full-scale audit on their program - and in some cases, it is! The fear that program representatives have with presenting to Safety Review Boards is the unknown. They may ask themselves:

What is going to be uncovered, or discovered?
Will they be able to provide sufficient responses to address questions and concerns and defend our safety assessments?
Will the Safety Review Board process delay the schedule?
Are they going to miss a test event milestone?
Will they make their Critical Design Review (CDR)?
Will they meet their certification process?
How much is this going to cost?
Why do they need to provide all of this Objective Quality Evidence (OQE)?

This paper provides tips and highlights what programs should do, and should not do, based on lessons learned to have successful Safety Review Board meetings. The end goal of any successful Safety Review Board meeting is to ensure the safety program processes and analytical artifacts are adequate and well-established to properly identify and assess safety risks for the personnel, equipment and environment that will be exposed to potential hazards during the system’s lifecycle.
Genome-wide identification and expression profile ofGhGRFgene family inGossypium hirsutum L.
Authors: Liu Kun , Kabir Nosheen , Wei Zhenzhen , Sun Zhuojing , Wang Jian , Qi Jing , Liu Miaoyang , Liu Ji , Zhou Kehai 
Published: May 2022

BackgroundCotton is the primary source of renewable natural fiber in the textile industry and an important biodiesel crop. Growth regulating factors (GRFs) are involved in regulating plant growth and development.MethodsUsing genome-wide analysis, we identified 35GRFgenes inGossypium hirsutum.ResultsChromosomal location information revealed an uneven distribution of GhGRF genes, with maximum genes on chromosomes A02, A05, and A12 from the At sub-genome and their corresponding D05 and D12 from the Dt sub-genome. In the phylogenetic tree, 35GRFgenes were divided into five groups, including G1, G2, G3, G4, and G5. The majority ofGhGRFgenes have two to three introns and three to four exons, and their deduced proteins contained conserved QLQ and WRC domains in the N-terminal end ofGRFsinArabidopsisand rice. Sequence logos revealed thatGRFgenes were highly conserved during the long-term evolutionary process. The CDS of theGhGRFgene can complement MiRNA396a. Moreover, mostGhGRFgenes transcripts developed high levels of ovules and fibers. Analyses of promoter cis-elements and expression patterns indicated that GhGRF genes play an essential role in regulating plant growth and development by coordinating the internal and external environment and multiple hormone signaling pathways. Our analysis indicated thatGhGRFsare ideal target genes with significant potential for improving the molecular structure of cotton.
The Microphenotron: a novel method for screening plant growth-promoting rhizobacteria
Authors: Raheem Asif , Ali Basharat 
Published: May 2022

Background
The ‘Microphenotron’ is an automated screening platform that uses 96-well microtiter plates to test the response of seedlings to natural products. This system allows monitoring the phenotypic effect of a large number of small molecules. Here, this model system was used to study the effect of phytohormones produced by plant growth-promoting rhizobacteria (PGPR) on the growth of wild-type and mutant lines of Arabidopsis thaliana.

Methods
In the present study, high-throughput screening based on ‘Microphenotron’ was used to screen PGPRs. Rhizobacteria were isolated from the rhizosphere of Acacia Arabica, which was growing in saline habitats. The phylogeny of these rhizobacteria was determined by 16S rRNA gene sequencing. Strains were screened for plant growth-promoting traits such as auxin production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, and phosphate solubilization. Ultra-Performance Liquid Chromatography (UPLC) was used to detect the presence of different indolic compounds. Finally, PGPR were evaluated to enhance the growth of A. thaliana in the ‘Microphenotron’ system and pot trials.

Results
Selected rhizobacteria strains showed positive results for multiple plant-growth promoting traits. For instance, strain (S-6) of Bacillus endophyticus exhibited the highest ACC-deaminase activity. UPLC analysis indicated the presence of different indolic compounds in bacterial extracts that included indole lactic acid (ILA), indole carboxylic acid (ICA), and indole-3-acetic acid (IAA). Two strains (S-7 and S-11) of Psychrobacter alimentarius produced the most IAA, ICA and ILA. A screening bioassay through 96-well microtiter plates with wild-type Col. N6000 showed an increase in root growth and proliferation. The highest twofold increase was recorded in root growth with B. thuringiensis S-26 and B. thuringiensis S-50. In pot trials, mutant lines of A. thaliana impaired for auxin signaling showed that B. endophyticus S-6, Psy. alimenterius S-11, Enterobacter asburiae S-24 and B. thuringiensis S-26 used auxin signaling for plant growth promotion. Similarly, for ethylene insensitive mutant lines (ein2.5 and etr1), Prolinoborus fasciculus S-3, B. endophyticus S-6, Psy. alimenterius S-7, E. asburiae S-24, and B. thuringiensis S-26 showed the involvement of ethylene signaling. However, the growth promotion pattern for most of the strains indicated the involvement of other mechanisms in enhancing plant growth. The result of Microphenotron assays generally agreed with pot trials with mutant and wild type A. thaliana varieties. Bacterial strains that induced the highest growth response by these cultivars in the ‘Microphenotron’ promoted plant growth in pot trials. This suggests that Microphenotron can accelerate the evaluation of PGPR for agricultural applications.