bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modelling somatic mutation accumulation and expansion in a long-lived tree with hierarchical modular architecture
Authors: Tomimoto Sou , Satake Akiko 
Published: May 2022

AbstractIn a long-lived organism with a modular architecture, such as trees, somatic mutations accumulate throughout the long lifespan and result in genetic mosaicism in each module within the same individual. In recent years, next-generation sequencing technology has provided a snapshot of such intra-organismal genetic variability. However, the dynamic processes underlying the accumulation and expansion of somatic mutations during the growth remain poorly understood. In this study, we constructed a model to describe these processes in a form that can be applied to a real tree. Given that the proliferation dynamics of meristematic cells vary across plant species, multiple possible processes for elongation and branching were comprehensively expressed in our model. Using published data from a poplar tree, we compared the prediction of the models with the observation and explained the cell lineage dynamics underlying somatic mutations accumulation that were not evident from the snapshot of the sequenced data. We showed that the somatic genetic drift during growth increases inter-meristem mosaicism, resulting in genetically distinct branches and less integrity within an individual tree. We also showed that the somatic genetic drift during branching leads to the mutation accumulation pattern that does not reflect the tree topology. Our modelling framework can help interpret and provide further insights into the empirical findings of genetic mosaicism in long-lived trees.
Multiple trade-offs between defense and competitiveness traits in a planktonic predator-prey system
Authors: Réveillon Tom , Becks Lutz 
Published: May 2022

AbstractPredator-prey interactions play a central role in community dynamics and depends on the covariation of traits of the interacting organisms. Intraspecific trade-off relationships between defense and competitiveness traits are important for understanding trophic interactions. However, quantifying the relevant traits forming defense-competitiveness trade-offs and how these traits determine prey and predator fitness remain major challenges. Here, we conducted feeding and growth experiments to assess multiple traits related to defense and competitiveness in 6 different strains of the green algaChlamydomonas reinhardtiiexposed to predation by the rotiferBrachionus calyciflorus.We found large differences in defense and competitiveness traits among prey strains and negative relationships between defense and competitiveness traits. Because we compared trait differences among strains whose ancestors previously evolved in controlled environments where selection favored defense or competitiveness, these negative correlations suggest the presence of a trade-off between defense and competitiveness. This trade-off was found for multiple combinations of defense and competitiveness traits. Furthermore, the differences in traits translated into differences in prey and predator fitness, which demonstrated the contribution of intraspecific trade-offs for predicting the outcome of predator-prey interactions.
Inhibition of c-Jun in AgRP neurons mediates chronic stress-induced anxiety-like behaviors and colitis susceptibility
Authors: Jiao Fuxin , Hu Xiaoming , Yin Hanrui , Yuan Feixiang , Zhou Ziheng , Wu Wei , Chen Shanghai , Liu Zhanju , Guo Feifan 
Published: May 2022

ABSTRACTPsychiatric disorders, such as anxiety, are frequently associated with inflammatory bowel diseases (IBD), however, the neural mechanisms are unknown. Here, we showed that hypothalamic agouti-related protein (AgRP) neuronal activity was suppressed under chronic restraint stress (CRS), a condition known to induce anxiety-like behaviors and increase colitis susceptibility. Consistently, chemogenic activation (inhibition) of AgRP neurons reversed (mimicked) CRS-induced anxiety-like behaviors and colitis susceptibility. Furthermore, CRS inhibited AgRP neuronal activity by suppressing the expression of c-Jun. As expected, overexpression of c-Jun in these neurons protected against the CRS-induced these effects and knockdown of c-Jun in AgRP neurons (c-JunΔAgRP) promoted anxiety-like behaviors and colitis. Moreover, relieving the anxiety with cyamemazine (an anxiolytic drug) alleviated colitis susceptibility in c-JunΔAgRP mice. Finally, according to a proteomic analysis, the levels of the secreted protein thrombospondin 1 (THBS1) were negatively associated with the increased anxiety-like behaviors and colitis susceptibility, supplementing recombinant THBS1 rescued colitis in c-JunΔAgRP mice. Taken together, these results reveal a critical role of hypothalamic AgRP neuron-derived c-Jun in orchestrating chronic stress-induced anxiety-like behaviors and colitis susceptibility. These results provide a new perspective for understanding the neuronal mechanisms and potential therapeutic target for the comorbidity of psychiatric disorders, such as anxiety, and IBD.
Minute-scale oscillatory sequences in medial entorhinal cortex
Authors: Cogno Soledad Gonzalo , Obenhaus Horst A. , Jacobsen R. Irene , Donato Flavio , Moser May-Britt , Moser Edvard I. 
Published: May 2022

AbstractThe medial entorhinal cortex (MEC) hosts many of the brain’s circuit elements for spatial navigation and episodic memory, operations that require neural activity to be organized across long durations of experience1. While location is known to be encoded by a plethora of spatially tuned cell types in this brain region2–6, little is known about how the activity of entorhinal cells is tied together over time. Among the brain’s most powerful mechanisms for neural coordination are network oscillations, which dynamically synchronize neural activity across circuit elements7–10. In MEC, theta and gamma oscillations provide temporal structure to the neural population activity at subsecond time scales1,11–13. It remains an open question, however, whether similarly powerful coordination occurs in MEC at behavioural time scales, in the second-to-minute regime. Here we show that MEC activity can be organized into a minute-scale oscillation that entrains nearly the entire cell population, with periods ranging from 10 to 100 seconds. Throughout this ultraslow oscillation, neural activity progresses in periodic and stereotyped sequences. This activity was elicited while mice ran at free pace on a rotating wheel in darkness, with no change in its location or running direction and no scheduled rewards. The oscillation sometimes advanced uninterruptedly for tens of minutes, transcending epochs of locomotion and immobility. Similar oscillatory sequences were not observed in neighboring parasubiculum or in visual cortex. The ultraslow oscillation of activity sequences in MEC may have the potential to couple its neurons and circuits across extended time scales and to serve as a scaffold for processes that unfold at behavioural time scales, such as navigation and episodic memory formation.
Spatial, Quantitative and Functional Deconstruction of Virus and Host Protein Interactions Inside Intact Cytomegalovirus Particles
Authors: Bogdanow Boris , Gruska Iris , Mühlberg Lars , Protze Jonas , Hohensee Svea , Vetter Barbara , Lehmann Martin , Wiebusch Lüder , Liu Fan 
Published: May 2022

ABSTRACTHerpesviruses assemble large enveloped particles that are difficult to characterize structurally due to their size, fragility and complex proteome with partially amorphous nature. Here we use cross-linking mass spectrometry and quantitative proteomics to derive a spatially resolved interactome map of intact human cytomegalovirus virions. This enabled thede novoallocation of 32 viral proteins into four spatially resolved virion layers, each organized by a dominant viral scaffold protein. The viral protein UL32 engages with all layers in an N-to-C-terminal radial orientation bridging nucleocapsid to viral membrane. In addition, we observed the layer-specific recruitment of 82 host proteins, a subset of which are constitutively and selectively incorporated via specific host-virus interactions. We uncover how the recruitment of PP1 phosphatase and 14-3-3 proteins by UL32 affects early and late steps during viral biogenesis. Collectively, this study provides global structural insights into the native configuration of virus and host protein interactions inside herpesvirus particles.
Addiction recovery stories: Paul Makin in conversation with Lisa Ogilvie
Authors: Makin Paul , Ogilvie Lisa 
Published: May 2022

Purpose
The purpose of this paper is to contribute to a series of recovery stories that examine candid accounts of addiction and recovery. Shared components in the recovery process are considered, along with the change and growth needed to support the transition from addiction to recovery.

Design/methodology/approach
The connectedness, hope, identity, meaning in life and empowerment (CHIME) framework comprises five elements important to recovery. CHIME provides a standard to qualitatively study recovery. As a model, it has been extended to include growth-CHIME (G-CHIME), an element that is important for sustained addiction recovery. In this paper, a first-hand account of addiction recovery is presented, followed by a semi-structured e-interview with the author of the account that is based on the G-CHIME model.

Findings
This paper shows that addiction recovery is a remarkable process that can be effectually explained using the G-CHIME model. The significance of each element in the model is apparent from the biography and e-interview presented.

Originality/value
To the best of the authors’ knowledge, each account of recovery in this series is unique and, as yet, untold.
Academic spinoffs team formation process: unfolding three micro-phases under the lens of effectuation and causation
Authors: Tagliazucchi Giulia , Marchi Gianluca 
Published: May 2022

PurposeBy using the lens of effectuation and causation, this paper aims at exploring how the team is formed in high-tech academic spinoffs, by looking specifically at decisional heuristics in an academic context. Indeed, the team composition is a critical issue for the subsequent growth of the academic new venture: on the one hand, the scientific-centred knowledge of the academic entrepreneurs is one of the main elements that lay the foundation for the new venture; on the other hand, it has been widely recognized that the lack of market-related knowledge and experience often has detrimental effects on performance. Decisive is then to explore how team decisions pertaining to the team formation process are taken.Design/methodology/approachA qualitative methodology based on multiple case studies is adopted under an abductive approach.FindingsResults shed light on how decisions pertaining to team formation are taken by academic entrepreneurs and with what effects on team composition, a fundamental element to foster the growth of academic new ventures. Specifically, this study derives some propositions about the adoption of effectuation and causation in the team formation process, their occurrence and sequence in the light of the scientific context in which academic new ventures spin out and the effects on the team composition.Originality/valueThis study contributes to the debate on academic entrepreneurs’ decisional heuristic and the use of effectuation or causation in the early stages of an academic new venture, by focusing on the team formation process. This study specifically considers three temporal micro-phases – the selection of founders before inception, the appointment of top management teams, and the integration of early employees after the inception – and whether the academic context influences the decisional heuristics. Managerial implications are also derived.
COVID-19 challenges and employees' stress: mediating role of family-life disturbance and work-life imbalance
Authors: Kundu Subhash C. , Tuteja Prerna , Chahar Purnima 
Published: May 2022

PurposeThe paper focuses on examining the relationship between challenges caused by COVID-19 pandemic and employees' stress through the mediating role of family-life disturbance and work-life imbalance.Design/methodology/approachA total of 1,556 respondents from 45 countries across all continents were included in this survey. Statistical techniques such as exploratory factor analysis (EFA), confirmatory factor analysis (CFA), correlations, multiple regression and bootstrapping were used to analyse the data.FindingsThe results depicted that COVID-19 challenges induced stress among employees. In combination with this, family-life disturbance and work-life imbalance mediated the relationship between COVID-19 challenges and employees' stress individually as well as serially. The paper further suggests some recommendations to mitigate the impact of COVID-19 challenges on employees' stress.Research limitations/implicationsThe major limitation of the study was in the data collection process. As the data were collected during the time period of pandemic, so, limited respondents were approached. Also, the international and cross-sectional design of the study can limit the applicability of the results.Practical implicationsThe study suggests organizations and HR managers to help in balancing family and work-life of employees, so that their mind can be kept stress free.Originality/valueThe study has added knowledge to the existing literature of stress created due to the COVID-19 pandemic by explaining the serial mediation of family-life disturbance and work-life imbalance between the relationship of COVID-19 challenges and employees' stress.
The effect of international remittances on labour supply in Jordan: an empirical investigation
Authors: Al-Assaf Ghazi 
Published: May 2022

PurposeThe purpose of the current study is to examine the effect of international remittances on the labour market participation of women and men left behind in Jordan. The study particularly focuses on the labour supply side for both women and men.Design/methodology/approachThe study draws on micro-data from the Jordan Labour Market Panel Survey (JLMPS) in 2010, a nationally representative survey, and addresses the endogeneity of receiving remittances through an Instrumental Variable (IV) approach.FindingsThe empirical results indicate that remittances are found to have a negative and significant impact on labour supply of both women and men. On average, women who live in remittance receiving household are about 5% points less likely to perform any market work, 3% points less likely to be in wage employment and about 8% points less likely to be engaged in own work. While, men who live in remittance receiving household are about 25% points less likely to perform any market work, 5% points less likely to be in wage employment and about 10% points less likely to be engaged in own work. When the author instruments for remittance receipt of the household, the effect of remittances on likelihood to work is found larger for both women and men.Originality/valueWorkers' remittances are considered as one of the vital financial sources for many households in labour exporting countries, most of the investigation on the effects of such financial flows concentrate on the macroeconomic effects. It is therefore important to conduct empirical investigation to fairly and accurately evaluate the impact of these flows on the Jordanian labour market at a microeconomic level.
Digital entrepreneurship: global maps and trends of research
Authors: Zhai Yuming , Yang Kaibo , Chen Lu , Lin Han , Yu Mingchuan , Jin Ruoyu 
Published: May 2022

Purpose
Digital technologies, such as big data and artificial intelligence, significantly impact entrepreneurial activities worldwide. However, research on entrepreneurial activities enabled by digital technologies is fragmented, divergent and delayed. This study aims to provide a structured review of digital entrepreneurship (DE) to identify status, hotspots, knowledge structure, dynamic trends and future developments in this field.

Design/methodology/approach
The bibliometric analysis was applied to offer a technological review on DE. In total 704 publications and their 34,083 references from Web of Science were retrieved as the sample set. Basic characteristics of publications, including the most influential documents, authors, journals and countries, were obtained. Then, co-citation and co-occurrence analyses were conducted to sketch the contours of the structure and evolution of DE.

Findings
DE has attracted increasing attention in the past three decades, especially after 2013. There are dozens of countries, hundreds of journals and more than 1,000 authors that have contributed to this field. Based on keyword co-occurrence clustering and co-citation clustering, the authors proposed a 3E (empower, evolution and ecosystem) framework of DE to facilitate an interdisciplinary dialogue for evidence-based policymaking and practice. In the future, researchers need to pay more attention to theoretical research and study DE from a holistic and dynamic perspective with consideration to the negative impact of digital technology on entrepreneurial activities.

Originality/value
This study draws an outline of the global advance on DE research. It presents an opportunity to comprehensively understand the contemporary achievements, the march of knowledge and the logical venation underlying academic developments as well as foundations for policymaking.
Messy talk: an unanticipated process of problem-solving and knowledge creation
Authors: Mandhana Dron M. 
Published: May 2022

PurposeThis paper discusses the communicative process of messy talk that enables collaborative problem-solving and tacit knowledge sharing among interdisciplinary team members. The paper aims to (1) trace relevant literature and lay out the conceptual and operational definitions of messy talk, (2) highlight messy talk as an enacted communication competence and (3) discuss the antecedents of messy talk and offer empirical propositions to guide future research.Design/methodology/approachThis conceptual paper traces and integrates relevant literature from the construction, management and organizational and group communication disciplines to promote and foster research on messy talk.FindingsBased on extant research on messy talk, the paper first provides clear conceptual and operational definitions of the messy talk construct. Second, using practice perspective, messy talk is presented as an enacted communication competence that focuses on the ongoing demands of the context and the situated practices of organizational members. Third, several factors including team members' technical expertise, task routineness, team history, time pressure and information sharing systems that influence the amount of messy talk conversations in teams are discussed. Lastly, the paper underscores the key implications of considering messy talk as an enacted communication competence on the performance and training of knowledge workers.Originality/valueThe presentation of messy talk as an enacted communication competence is a deliberate consideration of knowledge as an emergent, intersubjectively negotiated phenomenon that is deeply rooted in practice.
The mediating effect of fraud awareness on the relationship between risk management and integrity system
Authors: Sihombing Ranto Partomuan , Soewarno Noorlailie , Agustia Dian 
Published: May 2022

Purpose
Government institutions in Indonesia have implemented an integrity system as a strategy to prevent fraud and corruption by integrating the risk management and organizational ethics. This integration is important to increase the awareness of fraud in the organization. Based on self-determination theory, this study examines the mediating effect of fraud awareness on risk management and integrity systems.

Design/methodology/approach
The study was carried out by using a quantitative approach. The participants of the survey were auditors of the inspectorate of Ministries and Government Agencies in Indonesia. The number of respondents was 103 auditors. The hypothesis testing method used the partial least squares structural equation modeling (PLS-SEM) approach. The data were processed by using WarpPls 7.0 software.

Findings
There are two main results in this study. First, risk management directly affect the integrity of the system. Second, fraud awareness mediates the relationship between risk management and integrity systems.

Practical implications
The result of this study implicates the policymakers in Ministries and Government Agencies in Indonesia to increase organizational fraud awareness through the involvement of internal audits with risk management. The fraud awareness will greatly improve the performance of the integrity system.

Originality/value
This is the first study examined fraud awareness of integrity systems and risk management. This study can enrich the literature on internal audits, especially the duties of auditors with risk management.
Exploring changes in integration, differentiation, rules, coordination and performance following the introduction of a hospital planning centre: a case study
Authors: van der Ham Annelies , Van Raak Arno , Ruwaard Dirk , van Merode Frits 
Published: May 2022

PurposeThis study explores how a hospital works, which is important for further enhancing hospital performance. Following the introduction of a Hospital Planning Centre (HPC), changes are explored in a hospital in terms of integration (the coordination and alignment of tasks), differentiation (the extent to which tasks are segmented into subsystems), rules, coordination mechanisms and hospital performance.Design/methodology/approachA case study was conducted examining the hospital’s social network, rules, coordination mechanisms and performance both before and after the introduction of the HPC. All planning and execution tasks for surgery patients were studied using a naturalistic inquiry and mixed-method approach.FindingsAfter the introduction of the HPC, the overall network structure and coordination mechanisms and coordination mechanisms remained largely the same. Integration and certain rules changed for specific planning tasks. Differentiation based on medical discipline remained. The number of local rules decreased and hospital-wide rules increased, and these remained largely in people’s minds. Coordination mechanisms remained largely unchanged, primarily involving mutual adjustment and standardization of work both before and after the introduction of the HPC. Overall, the hospital’s performance did not change substantially. The findings suggest that integration seems to “emerge” instead of being designed. Hospitals could benefit, we argue, from a more conscious system-wide approach that includes collective learning and information sharing.Originality/valueThis exploratory study provides in-depth insight into how a hospital works, yielding important knowledge for further research and the enhancement of hospital performance.