bluuebunny/crossref_metadata_2025_split huggingface dataset:
The fate of recessive deleterious or overdominant mutations near mating-type loci under partial selfing
Authors: Tezenas Emilie , Giraud Tatiana , Véber Amandine , Billiard Sylvain 
Published: October 2022

AbstractLarge regions of suppressed recombination having extended over time occur in many organisms around genes involved in mating compatibility (sex-determining or mating-type genes). The sheltering of deleterious alleles has been proposed to be involved in such expansions. However, the dynamics of deleterious mutations partially linked to genes involved in mating compatibility are not well understood, especially in finite populations. In particular, under what conditions deleterious mutations are likely to be maintained for long enough near mating-compatibility genes remains to be evaluated, especially under selfing, which generally increases the purging rate of deleterious mutations. Using a branching process approximation, we studied the fate of a new deleterious or overdominant mutation in a diploid population, considering a locus carrying two permanently heterozygous mating-type alleles, and a partially linked locus at which the mutation appears. We obtained analytical and numerical results on the probability and purging time of the new mutation. We investigated the impact of recombination between the two loci and of the mating system (outcrossing, intra and inter-tetrad selfing) on the maintenance of the mutation. We found that the presence of a fungal-like mating-type locus (i.e. not preventing diploid selfing) always sheltered the mutation under selfing,i.e. it decreased the purging probability and increased the purging time of the mutations. The sheltering effect was higher in case of automixis (intra-tetrad selfing). This may contribute to explain why evolutionary strata of recombination suppression near the mating-type locus are found mostly in automictic (pseudo-homothallic) fungi. We also showed that rare events of deleterious mutation maintenance during strikingly long evolutionary times could occur, suggesting that deleterious mutations can indeed accumulate near the mating-type locus over evolutionary time scales. In conclusion, our results show that, although selfing purges deleterious mutations, these mutations can be maintained for very long times near a mating-type locus, which may contribute to promote the evolution of recombination suppression in sex-related chromosomes.
Glia-specific expression of neuropeptide receptor Lgr4 regulates development and adult physiology in Drosophila
Authors: Lee Seung Gee , Kaur Jasdeep , Miao Hongyu , Kim Woo Jae 
Published: October 2022

ABSTRACTSimilar to the human brain, Drosophila glia may well be divided into several subtypes that each carry out specific functions. Glial GPCRs plays key roles in crosstalk between neurons and glia. Drosophila Lgr4 (dLgr4) is a human relaxin receptor homolog involved in angiogenesis, cardiovascular regulation, collagen remodeling, and wound healing. Recent study suggests that ilp7 might be the ligand for Lgr4 and regulates escape behavior of Drosophila larvae. Here we demonstrate that Drosophila Lgr4 expression in glial cells, not neurons, is necessary for early development, adult behavior, and lifespan. Reducing the Lgr4 level in glial cells, but not neurons, disrupts Drosophila development, although knocking down other LGR family members in glia has no impact. Adult-specific knockdown of Lgr4 in glia but not neurons reduce locomotion, male reproductive success, and animal longevity. The investigation of how glial expression of Lgr4 contributes to this behavioral alteration will increase our understanding of how insulin signaling via glia selectively modulates neuronal activity and behavior.
Baculoviral COVID-19 Delta DNA vaccine cross-protects against SARS-CoV2 variants in K18-ACE2 transgenic mice
Authors: Jang Yuyeon , Cho Hansam , Chun Jungmin , Park Kihoon , Nowakowska Aleksandra , Kim Jinha , Lee Hyeondong , Lee Chanyeong , Han Yejo , Lee Hee-Jung , Shin Ha-Youn , Kim Young Bong 
Published: October 2022

AbstractAfter severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) made the world tremble with a global pandemic, SARS-CoV2 vaccines were developed. However, due to the coronavirus’s intrinsic nature, new variants emerged, such as Delta and Omicron, refractory to the vaccines derived using the original Wuhan strain. We developed an HERV-enveloped recombinant baculoviral DNA vaccine against SARS-CoV2 (AcHERV-COVID19S). A non-replicating recombinant baculovirus that delivers the SARS-CoV2 spike gene showed a protective effect against the homologous challenge in a K18-hACE2 Tg mice model; however, it offered only a 50% survival rate against the SARS-CoV2 Delta variant. Therefore, we further developed the AcHERV-COVID19 Delta vaccine (AcHERV-COVID19D). Cross-protection experiments revealed that mice vaccinated with the AcHERV-COVID19D showed 100% survival upon challenge with Delta and Omicron variants and 71.4% survival against prototype SARS-CoV2. These results support the potential of the viral vector vaccine, AcHERV-COVID19D, in preventing the spread of coronavirus variants such as Omicron and SARS-CoV2 variants.Author SummaryAfter the SARS-CoV2 pandemic, it is known that the existing vaccine has diminished efficacy against the emerging variants. We developed a baculoviral COVID19 DNA vaccine for the Delta variant (AcHERV-COVIS19D). Compared to AcHERV-COVID19S, designed to protect from the prototype of SARS-CoV2, AcHERV-COVID19D elicited higher humoral and cellular immunity and showed perfect protection against SARS-CoV2 delta strain and Omicron challenge. The broad and robust cellular immunity of the AcHERV-COVID19D vaccine appears to have played a significant role in the cross-protection of the Omicron variant. Our AcHERV-COVID19D can be a potential vaccine against emerging SARS-CoV2 variants.
Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factors
Authors: Wang Zhengqi , Emmel Grace , Lim Hong Seo , Zhu Wandi , Kosters Astrid , Ghosn Eliver E.B. , Qiu Peng , Bunting Kevin D. 
Published: October 2022

AbstractSignal transducer and activator of transcription 5 (STAT5a and STAT5b) are intrinsically critical for normal hematopoiesis but are also expressed in stromal cells. Here, STAT5ab knockout (KO) was generated with a variety of bone marrow hematopoietic and stromal Cre transgenic mouse strains. Vav1-Cre, the positive control for loss of multipotent hematopoietic function, surprisingly dysregulated niche factor mRNA expression and deleted STAT5ab in CD45neg cells. Single cell transcriptome analysis of bone marrow from wild-type or Vav1-Cre KO mice showed hematopoietic stem cell myeloid commitment priming and upregulated protein translation genes. Nes+ cells were detected in both CD45neg and CD45+ clusters and deletion of STAT5ab with Nes-Cre caused hematopoietic repopulating defects. To follow up on these promiscuous Cre promoter deletions in CD45neg and CD45+ bone marrow cell populations, more stroma-specific Cre strains were generated and demonstrated reduction in multipotent hematopoietic progenitors. Functional support for niche-supporting activity was assessed using STAT5-deficient MSCs. With Lepr-Cre, niche factor mRNAs were downregulated by STAT5ab deletion with validation of reduced IGF-1 and CXCL12 proteins. Furthermore, computational analyses (differential expression/co-expression) revealed a key role for STAT5ab/Cish balance with Cish strongly co-expressed in MSCs and HSCs primed for differentiation. Therefore STAT5ab-associated gene regulation supports the bone marrow microenvironment.
Evaluating rotations as a strategy to delay the evolution of insecticide resistance in vectors of human diseases
Authors: Hastings Ian , Jones Sam , South Andy 
Published: October 2022

AbstractIntroductionInsecticide resistance threatens the control of important human vector-borne diseases such as malaria and dengue. The current de facto strategy is to target the insect vectors using a sequential deployment of insecticides i.e. use one insecticide (usually the cheapest available) until resistance has made it ineffective and then replace it with the next insecticide in the repertoire. Rotations of insecticides are often advocated as a potentially superior method of using the insecticide repertoire to delay the evolution of resistance. Testing this hypothesis in the field is logistically demanding and an in silico approach offers a much faster, flexible and transparent method of evaluating rotations.MethodsWe develop an in silico approach to evaluate rotations using sequential deployment as the baseline. We explored a wide range of deployment scenarios, underlying genetics of resistance, and incorporated costs of resistance and gene flow to/from untreated refugia.ResultsWe found that, under most circumstances, resistance to all the insecticides in the repertoire were reached at very similar times for rotations and sequences. Any advantages of one strategy over the other tended to be small (typically <10%) and unpredictable.ConclusionsOperational factors, such as cost or supply-chain security, may therefore largely determine the optimal choice of insecticide deployment strategies.
The impact of the COVID-19 pandemic in higher education: a gender perspective
Authors: Hamadeh Randah R , AlSabbagh Maha , Bugawa Afaf Mubarak , Kamal Adel , Ali Fuad , Al Bufalasa Ghada Abdulla , AlShaibani Tarik 
Published: October 2022

PurposeThe study will inform relevant workplace gendered policies at the university and other academic institutions.Design/methodology/approachA cross -sectional study was conducted on all the Arabian Gulf University full-time employees during the COVID-19 pandemic, in June 2021. A questionnaire was sent to the employees requesting data that included sociodemographic data, living conditions, psychological status, social aspects, work productivity and satisfaction. The generalized anxiety disorder questionnaire (GAD-7) was used to screen for anxiety.FindingsGender disparity was noted in the increase of responsibilities at home and towards young children, and in stress and anxiety. Women’s reductions in peer (90.7%) and social (88%) interactions were higher than among males (81.8%, 73%, respectively). There was a narrow gender gap in work productivity and workload. More males reported that the university was concerned about its employees during COVID-19 and were satisfied with the measures taken. There was a gender disparity with respect to superiors periodically checking if the workload was manageable and discussing the mental health and well-being of the employees.Research limitations/implicationsFuture research directions may focus on how gender impacts the perception and response to crises in higher education in Arab countries.Originality/valueThis is one of the few studies in the region that examined the gendered psychosocial impact of COVID-19 and productivity in the workplace using a higher education institution as an example.
M-PoP: leveraging the systematic modeling of processes-of-business processes
Authors: Cagnin Maria Istela , Nakagawa Elisa Yumi 
Published: October 2022

PurposeThis paper presents M-PoP, a method to model large, complex, and dynamic business processes. These processes have sometimes resulted from alliances of organizations (i.e. joint ventures and mergers and acquisitions) and are referred to as Processes-of-Business Processes (PoP). Due to the difficulty of modeling these dynamic processes, alliances of organizations have often lost opportunities, competitiveness, and profitability, so requiring suitable modeling methods.Design/methodology/approachThe authors proposed M-PoP that can model PoP through three views in different abstraction levels and using well-known techniques in industry and academia, mainly those from Business Process Model and Notation (BPMN). For this, M-PoP presents three main steps: identification of PoP elements, modeling of PoP, and verification of PoP models. To evaluate M-PoP, we applied it in a real-world business process in the health domain.FindingsThe evaluation results point out the capability and viability of M-PoP to deal with dynamic business processes.Research limitations/implicationsM-PoP still needs to be applied in various real-world scenarios to gather evidence of its productivity, efficiency, and scalability.Practical implicationsThis novel method could change the way organizations model their business processes and, as a consequence, it could leverage strategic business opportunities.Originality/valueM-PoP is the first method that makes it possible to model large and complex business processes and, most importantly, dynamic processes.
Customer responses to the Airbnb platform: the role of Airbnb experience and memorability
Authors: Li Jing , So Kevin Kam Fung , Hudson Simon 
Published: October 2022

Purpose
Guided by memory-dominant logic, this study aims to examine how Airbnb experiences (i.e. home benefits, social interaction and authenticity) lead to memorability, which subsequently results in platform attachment and loyalty. The mediating role of memorability and the moderating role of arousal in forming platform loyalty were also investigated.

Design/methodology/approach
A quantitative method was adopted including the use of a national survey with US travelers who had an Airbnb accommodation experience in the past six months. Data were collected from a representative sample generated from the Qualtrics consumer panel using an online self-administered survey.

Findings
Results suggested that Airbnb experiences have a positive impact on memorability, which leads to platform attachment and loyalty. The findings further supported the full mediation. The results also revealed a moderating role of arousal in influencing the relationship between social interaction and memorability.

Research limitations/implications
This study contributes to the literature by investigating the role of Airbnb experiences and memorability in influencing customer responses to the Airbnb platform. This study also provides a holistic understanding of how platform attachment and loyalty develop through the mechanism of memorability.

Practical implications
Given the continuous competition from hotels, Airbnb providers should continue to improve critical Airbnb attributes and balance its hedonic and functional values. The moderating role of arousal suggests that Airbnb could maintain a virtual community or a social platform where guests can interact with hosts or the locals before, during and after Airbnb experiences.

Originality/value
The findings provide a holistic understanding of the experiences with insight into the new business model of the sharing economy.
A systematic review of trust in sharing accommodation: progress and prospects from the multistakeholder perspective
Authors: Song Xiaoxiao , Gu Huimin , Li Yunpeng , Ye Weijiao 
Published: October 2022

Purpose
Trust has emerged as a crucial research topic in the sharing economy. However, scholarship on trust in sharing accommodation remains limited. By using stakeholder theory, this study aims to provide a systematic framework for integrating trust among multiple stakeholders and identify potential knowledge gaps and future research directions for trust in sharing accommodation.

Design/methodology/approach
The authors select papers using a combination of multiple keywords from EBSCOhost and Web of Science. The analysis includes 172 journal papers published between 2011 and 2021. The authors conduct a systematic review through thematic content analysis, and each paper is analyzed using manual coding.

Findings
The analysis shows that key stakeholders for trust building in sharing accommodation include consumers, hosts, platforms, residents and governments, with most studies focusing on the consumer perspective. The study integrates various trust antecedents and outcomes from the above multistakeholder. Second, this study summarizes the most commonly used theories, and more diversified theories could be applied to future research. Third, this study finds that most studies use quantitative methods, and researchers should introduce more integrated methodologies such as machine learning on a large scale. Furthermore, the current research disciplinary paradigm should be extended to multidisciplinary and interdisciplinary approaches to promote innovation in trust research. Finally, the COVID-19 pandemic has brought both challenges and opportunities to industry as well as researchers, and more institutional rather than commercial perspectives need to be addressed.

Research limitations/implications
The study contributes to the trust and the sharing economy literature by providing a systematic framework for integrating trust from multistakeholder perspectives. The study also points out several future research directions by combining micro and macro multistakeholder perspectives, identifying more diversified theories and methodologies and specifying multidisciplinary and interdisciplinary approaches.

Originality/value
The study advances knowledge by providing a systematic framework for integrating trust among multiple stakeholders and proposing future research directions for trust in sharing accommodation.
Flood susceptibility mapping in anthropogenic wetland area, Shadegan County, Southwest of Iran
Authors: Khaleghi Somaiyeh , Jadmavinejad Ahmad 
Published: October 2022

Purpose
Shadegan County as a wetland area was selected because of its susceptibility to flooding hazards and inundation. The purpose of this paper is to analyze flooding hazard based on the analytical hierarchy process methodology.

Design/methodology/approach
The eight influencing factors (slope, distance from wetland, distance from river, drainage density, elevation, curve number, population density and vegetation density) were considered for flood mapping within the Shadegan County using analytical hierarchical process, geographical information system and remote sensing. The validation of the map was conducted based on the comparison of the historical flood inundation of April 21, 2019.

Findings
The results showed that around 32.65% of the area was under high to very high hazard zones, whereas 44.60% accounted for moderate and 22.75% for very low to the low probability of flooding. The distance from Shadegan Wetland has been gained high value and most of the hazardous areas located around this wetland. Finally, the observed flood density in the different susceptibility zones for the very high, high, moderate, low and very low susceptible zones were 0.35, 0.22, 0.15, 0.19, and 0.14, respectively.

Originality/value
To the best of the authors’ knowledge, the flood susceptibility map developed here is one of the first studies in a built wetland area which is affected by anthropogenic factors. The flood zonation map along with management and restoration of wetland can be best approaches to reduce the impacts of floods.
Linking entrepreneurial orientation with export performance: mediation effects of multiple differentiation strategies
Authors: Hossain Kamal , Che Abdullah Ahmad Sufian , Mohd Balwi Mohd Abd Wahab Fatoni , Lubis Asmuliadi , Azizan Noor Azlinna , Alam Mohammad Nurul , Taha Azni Zarina 
Published: October 2022

Purpose
This study aims to examine the effect of entrepreneurial orientation (EO) on the export performance of apparel small- and medium-sized enterprises (SMEs) and the role of multiple differentiation strategy as a mediation effect between their relationships. It has also investigated the moderation impact of export market category between EO and performance relationship. The multiple differentiation strategy comprises the product (PDD), customer (CTD), service (SVD) and brand (BDD) differentiations.

Design/methodology/approach
A cross-sectional survey was carried out by providing a questionnaire to senior managers and owners of the apparel SMEs from the developed and developing markets exporters. The primary data of 550 was treated by the partial least squares-structural equation modelling) technique for final analysis.

Findings
The study revealed EO’s positive and significant effect on SMEs’ export performance. The study has found the mediation effect of product, customer and brand differentiation strategies between EO and export performance relationships from the mediation analysis. In contrast, service differentiation has found no mediation effect. However, the moderation effect (export market category) has revealed an insignificant effect between EO and performance association.

Research limitations/implications
The findings of this study are based on one country data analysis. This study has been conducted in the SMEs of the apparel industry in Bangladesh, considering only owners and senior-level managers of the firms.

Originality/value
This research has drawn the attention of managers/owners to EO and multiple differentiation strategies enhancing export performance from the developing country context, such as Bangladesh. Multiple differentiation as a competitive strategy is the pioneer application of mediating effect between EO and export performance relationships. Moreover, this research has investigated the effect of the export market category as a moderator. Dearth research has applied the export market category to investigate the moderation effect between EO-performance models. Therefore, current research has theoretical and practical contributions to the international entrepreneurship and strategic management literature.
Negotiating digital public spaces: context, purpose and audiences
Authors: Bettivia Rhiannon Stephanie , Stainforth Elizabeth 
Published: October 2022

PurposeThe purpose of this article is to investigate digital public spaces and audiences and to explore the relationship of digital public spaces to both ideas of nationhood and physical public institutions.Design/methodology/approachThe article investigates tensions arising from the conjuncture of public spaces and digital culture through the lens of the Digital Public Library of America (DPLA). This research uses qualitative content analysis of a range of data sources including semi-structured interviews, primary texts and secondary texts.FindingsThe construction of the public library space as a digital entity does not attract anticipated audiences. Additionally, the national framing of the DPLA is not compatible with how audiences engage with digital public spaces.Originality/valueDrawing on original, qualitative data, this article engages with the prevalent but undertheorized concept of digital public spaces. The article addresses unreflexive uses of the digital public and the assumptions connected to the imagined audiences for platforms like the DPLA.