bluuebunny/crossref_metadata_2025_split huggingface dataset:
ΔNp63α Transcriptionally Represses p53 Target Genes Involved in the Radiation-Induced DNA Damage Response
Authors: Kudo Ken-ichi , Tsuyama Naohiro , Nagata Kento , Imaoka Tatsuhiko , Iizuka Daisuke , Sugai-Takahashi Misaki , Muramatsu Moe , Sakai Akira 
Published: June 2022

Abstract
Background: The DNA damage response (DDR) is a mechanism that protects cells against radiation-induced oxidative DNA damage by causing cell cycle arrest and apoptosis. TP63 is a member of the tumour suppressor TP53 gene family, and ΔNp63α, a TP63 splicing variant, is constitutively expressed in the stem cell-containing basal layer of stratified epithelial tissues, including the mammary gland, where it plays a critical role in stemness and tissue development. ΔNp63α has been reported to transcriptionally inhibit the tumour suppression protein p53. This p53-repressive activity may cause genomic instability in epithelial stem cells exposed to radiation. In this study, we analysed the inhibitory effect of ΔNp63α on radiation-induced DDR.Methods: To elucidate the role of the p53-repressive effect of ΔNp63α in radiation response, we performed a p63-siRNA knockdown experiment using human mammary epithelial cells (HMECs) expressing ΔNp63α and then performed ectopic and entopic expression experiments using human induced pluripotent stem cells (hiPSCs). After irradiation, the expression of DDR-related genes and proteins in ΔNp63α-expressing and control cells was analysed by RT–qPCR, Western blotting, and flow cytometry.Results: The mRNA/protein expression levels of BAX and p21 were significantly increased in p63-siRNA-treated HMECs (sip63) after X-ray irradiation (4 Gy, 0.7 Gy/min) but not in scramble-siRNA treated HMECs (scr). Transcriptomic analysis showed decreased RNA expression of cell cycle-related genes and increased expression of programmed cell death-related genes in sip63 cells compared to scr cells. Furthermore, flow cytometric analysis revealed an increase in apoptotic cells and a decrease in 5-ethynyl-2´-deoxyuridine uptake in sip63 cells compared to scr cells. On the other hand, both the ectopic and entopic expression of ΔNp63α in apoptosis-sensitive hiPSCs reduced the expression levels of BAX after irradiation and significantly decreased the number of apoptotic cells induced by radiation.Conclusions: Taken together, these results indicate that ΔNp63α represses p53-related radiation-induced DDR, thereby potentially causing genomic instability in epithelial stem cells.Trial registration: None
Chemical Composition, Storage Stability and Microbial Quality of Debittered Orange Seed Flour
Authors: Emojorho Ernest Eguono , Okonkwo Thomas Mmuoemene 
Published: June 2022

Abstract
The study was carried out to determine the effect of debittering methods on the chemical, microbiological and shelf-life of defatted and undefatted orange seed flours. The orange seeds were removed manually from sweet orange fruits, soaked in water for 12 hours and then boiled at 100 0C for 30, 60, 90, 120 and 150 min, respectively. The boiled seeds were dehulled manually, ground, and a portion defatted with absolute ethanol, milled and sieved. The various flours were analyzed for chemical composition, shelf life and microbiological properties. The orange seed flours contained 8.73–17.66% amylose, 11.71–33.43% amylopectin, 20.44–43.95% starch, 2.17–3.01 g/100g histidine, 3.4–5.11 g/100g, 6.65–8.22 g/100g leucine, 3.40–4.61 g/100g lysine, 1.20–4.61 g/100g methionine, 3.37–4.97 g/100g phenylalanin, 2.39–4.15 g/100g treonine, 1.16–1.58 g/100g tryptophan, 4.03–5.5 g/100g valine, 3.34–4.63 g/100g alanine, 8.16–10.84 g/100g arginine, 7.57–10.05 g/100g aspartic acid, 0.60–1.45 g/100g cystine, 9.54–13.63 g/100g glutamic acid, 2.84–5.04 g/100g proline, 3.19–5.03 g/100g serine, 2.92–4.49 g/100g tyrosine, 1.25–18.42 µg/g limonene. The limonene which is responsible for the bitter taste of the orange seed reduced with increase in the boiling time. Peroxide value, total viable count and titratable acidity of the biscuits increased with storage, while the pH deceased with storage period.
Investigation On the Suitability of Metamorphosed Granite In Ilorin Nigeria For Road Construction and Polished Rock
Authors: Olatunjji K.J. , Kamilu D.O. , Oladiti A. G. , Akanji F.A. , Ibrahim A.O. , Abubakar A. A. , Kolawole M.F. 
Published: June 2022

Abstract
The suitability of metamorphosed granite for road construction and polished rock were investigated. Three samples A, B and C were handpicked from Man Hardi Quarry Ilorin using random sampling method. The samples were subjected to different test namely, chemical analysis, polish value, oxidation properties, compressive strength and hardness test. The chemical analysis shows the silica contents for samples A, B and C are 67.73%, 73.62% and 72.40% respectively, specific gravity obtained are 4.52, 4.80 and 3.60 for sample A, B and C. The oxidation properties test reported with no peeling was observed, the polish stone value (psv) test for the samples resulted in good abrasiveness and produce attractive colour. The values for samples A, B and C are 90.88, 97.36 and 93.71 while respectively compressive strength test estimation obtained were 142.37, 201.62 and 164.62 for sample A, B and C. The Hardness test values are 85.59, 96.84 and 88.11 respectively. Hence, the value obtained from the investigation on the suitability of metamorphosed granites for road construction and polish rock shows that they are good for constructional and polished stonework.
Safety and efficacy of 8-and 10-sector ultrasound cycloagulation: a retrospective study
Authors: Rong Zhang , Li Fuhua 
Published: June 2022

Abstract
Purpose: To measure the efficacy and safety of ultrasound cycloplasty (UCP) in sectors 8 and 10.Methods: Thirty-four patients with various types and degrees of glaucoma (43 eyes) underwent UCP at the Aier Eye Hospital of Tianjin University. Primary outcomes included intraocular pressure and visual acuity. Secondary outcomes included corneal endothelium, pupil area, cornea (Surface asymmetry index）SAI and(Surface regularity index) SRI , ciliary body detachment, and the safety and efficacy of UCP after trabeculectomy.Results: Twelve months postoperatively, intraocular pressure (IOP) decreased from 28.4 mmHg to 14.4 mmHg (P < 0.05). On average, IOP decreased by 44.7%, and the success rate was 55.8%. At the final follow-up, IOP decreased by 43.46% on average, and the success rate was 62.8%. There was a significant difference in the postoperative pupil area of the eight sectors (P = 0.016) within six months and no significant difference after six months. There was a significant difference in SRI between baseline and one month after UCP (P = 0.016) and no significant difference in the remaining months. There was no significant difference in corneal endothelium from the preoperative baseline. Ciliary body detachment occurred in sectors 8 and 10, and it was removed and reduced one month after surgery. The success rate of UCP after trabeculectomy was 91%, and there were no follicle-related complications.Conclusion: UCP is safe and significantly lowers IOP, with few short-term complications and no long-term complications. It is safe and effective to select sectors 10 and 8 for IOP reduction; however, there is no advantage in IOP reduction compared with sector 6.
Ameliorative Effects of Ethanolic leaf extract of Diodia sarmentosa on high fat induced cardiac tissue injury in male wistar albino rats
Authors: Korie Stephen Chinedumije , Ezejiofor Tobias I.N , Ezeji Uche E. 
Published: June 2022

Abstract
Background
Diodia sarmentosa is a native straggling herb found in Nigeria, especially in evergreen forest, bushland, and riverine areas. In folklore medicine, it has been used to treat ailments like diabetes, ulcers, etc. This study investigated the ameliorative effects of ethanolic leaf extract of Diodia sarmentosa on high-fat diet-induced cardiac tissue injury in male albino Wistar rats.
Methods
Thirty (30) male Wistar rats (150g-200g) were divided into five (5) groups namely; Negative control (NC) group which was not induced nor treated, Positive control (PC) was induced but not treated, Low dose extract (LDE) was treated with 250mg/kg of the extract, High dose extract (HDE) was treated with 500mg/kg of the extract and SIM group treated with 5mg/kg of Simvastatin. Cardiac tissue injury was induced by feeding the rats with high-fat diet (Ghee and Coconut oil in the ratio of 3:1) for six (6) weeks. The administration of the treatments (extract and drug) at the appropriate doses started from the 4th week till the 6th week. Parameters like Total protein concentration, Lactate dehydrogenase (LDH) activity, Malondialdehyde level, Superoxide dismutase (SOD) activity, Catalase (CAT) activity, Glutathione S-transferase (GST) activity, and Glutathione peroxidase (GPx) activity were carried out in the cardiac tissue of the test rats to determine the ameliorative effects of Diodia sarmentosa.
Results
Results obtained showed that high-fat diet reduced antioxidant enzymes, protein levels, and caused lipid peroxidation indicating injury in the cardiac tissue of the test rats. However, the extract at high dose significantly reduced (p < 0.05) lipid peroxidation and high GST activity in the cardiac tissue caused by high-fat diet. Protein levels and antioxidant enzyme activities in SOD, CAT, GPx, and LDH were all significantly increased (p < 0.05), when compared to the positive group. The extract at high dose possesses similar efficacy to the standard drug simvastatin used to compare the efficacy of the extract.
Conclusions
Thee evidence from this study shows Diodia sarmentosa has the potential to improve and reverse the injury in the cardiac tissue caused by high-fat diet administration in the test rats, by reducing lipid peroxidation, and increasing antioxidant enzymes and total protein concentration. This makes the plant a potential new natural product for the treatment of cardiovascular diseases.
Prognostic significance of preoperative nutritional assessment in elderly patients who underwent laparoscopic gastrectomy for stage I–III gastric cancer
Authors: Uehara Hideo , Ota Mitsuhiko , Yamamoto Manabu , Nakanoko Tomonori , Shin Yuki , Shiokawa Keiichi , Fujimoto Yoshiaki , Nakashima Yuichiro , Sugiyama Masahiko , Onishi Emi , Shimagaki Tomonari , Mano Yohei , Sugimachi Keishi , Morita Masaru , Toh Yasushi 
Published: June 2022

Abstract
Purpose
Surgery for elderly patients with gastric cancer is becoming more common. However, the risk factors of the laparoscopic surgery for these patients are unknown, and thus it is difficult to determine appropriate treatments for such patients.
Methods
Two hundred twenty-two patients who underwent laparoscopic gastrectomy for primary gastric cancer between January 2014 and December 2017 were enrolled. Clinical characteristics and short- and long-term prognoses were analyzed in 47 patients aged 75 years or older (elderly group) and in 175 patients who were under 75 years old (non-elderly group).
Results
The presence of comorbidities was more common in the elderly group than in the non-elderly group (91.5% versus 61.7%, p < 0.0001). The rate of postoperative complications in the elderly group was significantly higher than that in the non-elderly group (42.6% vs 22.9%, p = 0.01). The 5-year overall survival rate was significantly lower in the elderly group than in the non-elderly group (66.9% versus 92.2%; p < 0.0001). In the elderly group, 5-year overall survival in patients with a low preoperative prognostic nutritional index (PNI) was significantly worse than that in patients with a high preoperative PNI (25.0% versus 80.9%; p < 0.05). Multivariate analysis showed that the PNI value was independently associated with overall survival in elderly patients who underwent laparoscopic gastrectomy (p < 0.05). In particular, the rate of non-cancer deaths after surgery in elderly patients was significantly higher than that in non-elderly patients (p < 0.05).
Conclusion
PNI value is an independent prognostic factor for overall survival in elderly patients who have undergone laparoscopic gastrectomy for gastric cancer; therefore, we need to determine not only its recurrence but also non-cancerous diseases in elderly patients with low PNI.
An Immunoinformatics Study to Explore HTL Epitopes for Fungal Pathogen Aspergillus lentulus
Authors: Dubey Shreesh Kumar , Rajawat Anuradha , Gupta Harsh Mohan , Kumar Ajay 
Published: June 2022

Abstract
Purpose
Aspergillus lentulus causes invasive aspergillosis and responsible for harmful impact on human health, as targets pulmonary sites and causes impaired respiration. Aspergilli mycotoxins too have a significant negative influence on human and animal health. Here, we found T-cell epitopes from proteome of Aspergillus lentulus and targeted them against MHC-Class II HLA determinants to predict putative immunogenic nature. Here we used best immunoinformatics tools like PatchDock, NetMHCII server, Molprobity, ProSA, ToxinPred and ProtParam to conduct the epitopes screening and to construct a novel scheme of epitope structural and functional working. The immunoinformatics study was found to be very novel method in rapid designing of vaccines; it is an efficient way to reduce hit and trial approaches. Here we finalized four epitopes GENGVNQLQGTTLGR, ITNYFSTQKLGSSSQ, YITNYFSTQKLGSSS, and PADYTSTARALSLPM, that interacts with MHC Class II alleles and were found to show better population coverage and stability in the docked complexes. In current study we targeted membrane proteins of Aspergillus lentulus fungal pathogen, it was very less studied but found to be involved in producing harmful pulmonary impacts on human and animals. The immunoinformatics study was found to be very novel method in rapid designing of vaccines; it is an efficient way to reduce hit and trial approaches. Here we finalized four epitopes against this harmful pathogen that can elicit immune responses in host.
An Improved Deep Convolutional Neural Network by Using Hybrid Optimization Algorithms to Detect and Classify Brain Tumor Using Augmented MRI Images
Authors: Qader Shko M. , Hassan Bryar A. , Rashid Tarik A. 
Published: June 2022

Abstract
Automated brain tumor detection is becoming a highly considerable medical diagnosis research. In recent medical diagnoses, detection and classification are highly considered to employ machine learning and deep learning techniques. Nevertheless, the accuracy and performance of current models need to be improved for suitable treatments. In this paper, an improvement in deep convolutional learning is ensured by adopting enhanced optimization algorithms, Thus, Deep Convolutional Neural Network (DCNN) based on improved Harris Hawks Optimization (HHO), called G-HHO has been considered. This hybridization features Grey Wolf Optimization (GWO) and HHO to give better results, limiting the convergence rate and enhancing performance. Moreover, Otsu thresholding is adopted to segment the tumor portion that emphasizes brain tumor detection. Experimental studies are conducted to validate the performance of the suggested method on a total number of 2073 augmented MRI images. The technique's performance was ensured by comparing it with the nine existing algorithms on huge augmented MRI images in terms of accuracy, precision, recall, f-measure, execution time, and memory usage. The performance comparison shows that the DCNN-G-HHO is much more successful than existing methods, especially with a scoring accuracy of 97%. Additionally, the statistical performance analysis indicates that the suggested approach is faster and utilizes less memory at identifying and categorizing brain tumor cancers on the MR images. The implementation of this validation is conducted on the Python platform. The relevant codes for the proposed approach are available at: https://github.com/bryarahassan/DCNN-G-HHO.
Chromosomal aberrations: A cytogenetic study of suspected cases in North Indian populations.
Authors: Kashayap Aditya , Ranjan Prashant , Sarkar Mou , Rai Amit Kumar , Das Parimal 
Published: June 2022

Abstract
Introduction Every year, a considerable number of children with genetic diseases are born in India over half a million with deformities, and another 21,000 with Down syndrome, owing to the country’s high birth rate. Recurrent abortions (12.4%), recognizable syndromes (12.1%), chromosomal anomalies (11.3%), and mental retardation were the top four diseases identified in multi-centric research findings on cases referred for genetic counseling (11%).ObjectiveTo study the prevalence of chromosomal anomalies in hospitals, captured suspected patients from the North Indian population.Material and MethodsBlood lymphocyte culture was used for metaphase chromosome preparation. The G-banded karyotypes of 244 suspected patients referred by IMS and BHU during the last five months (December 2021 to April 2022) were analyzed to study the chromosomal anomalies.Results Out of the all chromosomal abnormality, 64.58 % were cases of Down syndrome, and 14.58 % of the cases had Philadelphia chromosomes. 10.41 % had Klinefelter syndrome and 4.10% of cases are Turner. However, 2% of the cases were XXXY, X q arm deletion, and mosaic form. Based on these retrospective study observations, the prevalence of chromosomal anomalies in suspected hospitalized patients of the North Indian population was calculated as 19.67%.ConclusionThe prevalence of chromosomal anomalies in the north Indian population may differ greatly from data from the United States. Chromosomal abnormalities can be diagnosed and managed more quickly with cytogenetic testing methods.
WITHDRAWN: Effect of temperature factors on the heat conduction properties of carbon nanotubes
Authors: Chen Junjie 
Published: June 2022

Abstract
The effect of temperature on the thermal conductivity of carbon nanotubes was studied theoretically to understand the heat transport phenomena occurring in the nanostructured materials. The thermal conductivity of macroscopic carbon nanotubes was determined by employing the nonequilibrium molecular dynamics method. The effects of different temperature factors on the heat conduction properties were investigated to evaluate the roles of phonon scattering and defects in the thermal conductivity of the nanostructured materials. The results indicated that macroscopic carbon nanotubes can conduct heat very efficiently, depending on a variety of temperature factors. The structure and allotropes play fundamentally important roles in determining the heat conduction properties of carbon nanotube-based materials. Macroscopic materials formed by single-walled carbon nanotubes typically possess high heat conduction properties. The phonon scattering and defects negatively affect the heat conduction properties because of the low degree of the mean free path of phonons. Carbon nanotube-based materials give rise to a power-law dependence of thermal conductivity with respect to temperature. The lattice thermal conductivity of carbon nanotube-based materials first increases and then decreases with the temperature. Low levels of defects can compensate for the adverse effect of strong phonon scattering on lattice thermal conductivity. A maximum thermal conductivity is achieved at low temperatures, but Umklapp scattering occurs. The thermal conductivity of graphene is significantly higher than that of carbon nanotube-based materials because of the increased degree of ballistic thermal conductance.
Sustainable magnetically recoverable Iridium coated Fe3O4 nanoparticles for enhanced catalytic reduction of organic pollutants in water
Authors: kumari Diksha , Kaur Manpreet , Yempally Veeranna , Kaur Harminder 
Published: June 2022

Abstract
In the present study, modified Fe3O4 nanoparticles were fabricated by surface coating with tryptophan and Iridium by co-precipitation method to afford Fe3O4@trp@Ir magnetic nanoparticles. As-prepared Fe3O4@trp@Ir nanoparticles are environmentally benign efficient catalysts for reducing organic pollutants such as 4-nitrophenol (4-NP), 4-nitroaniline (4-NA), and 1-bromo-4-nitrobenzene (1-B-4-NB). The key parameters that affect the catalytic activity like temperature, catalyst loading, and the concentration of reducing agent NaBH4 were optimized. The obtained results proved that Fe3O4@trp@Ir is an efficient catalyst for reducing nitroaromatics at ambient temperature with a minimal catalyst loading of 0.0025%. The complete conversion of 4-nitrophenol to 4-aminophenol took only 20 seconds with a minimal catalyst loading of 0.0025% and a rate constant of 0.0522 s− 1. The high catalytic activity factor (1.040 s− 1mg− 1) and high turnover frequency (9 min− 1) obtained for Fe3O4@trp@Ir nanocatalyst highlight the possible synergistic effect of the two metals (Fe and Ir). The visible-light photocatalytic degradation of 4-NP was also investigated in the presence of Fe3O4@trp@Ir. The photocatalytic degradation of 4-NP by Fe3O4@trp@Ir is completed in 20 min with 95.15% efficiency, and the rate of photodegradation of 4-NP (0.1507 min− 1) is about twice the degradation rate of 4-NP in the dark (0.0755 min− 1). The catalyst was recycled and reused for five cycles without significant reduction in the conversion efficiency of the catalyst.
Economic Prosperity, Asymmetric Natural Resource Income, and Ecological Demands in Resource-reliant Economies
Authors: Aladejare Samson Adeniyi 
Published: June 2022

Abstract
This study extends the literature by examining the linear and nonlinear effects of natural resource income and the contribution of environmental demands to long-term economic growth in resource-reliant economies (RREs). Data was sourced from 45 RREs from 1970 to 2019 and processed using three novel methodological techniques: the mean group (MG), augmented MG, and common correlated effects MG. Empirically, the study affirmed asymmetry in the natural resources rents-economic growth nexus. Specifically, the study found that environmental demands and positive changes to natural resources income are a blessing to long-term economic growth. In contrast, adverse changes to natural resources income are growth decelerating. Globalisation and human capital development served as control variables, and while the former accelerated long-term economic growth, the latter had an irrelevant effect on the RREs. In addition, the Dumitrescu and Hurlin causality result showed a bidirectional nexus between environmental quality and economic growth and between economic growth and negative natural resource income changes. A unidirectional causal flow from positive natural resources income to economic prosperity and from the latter to human capital development was also revealed. The study proposed relevant policy measures.