bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adult Vaccination in the United Arab Emirates—A Physicians' Knowledge and Knowledge Sources Study
Authors: Barqawi Hiba J. , Samara Kamel A. , Hassan Mahmoud S. , Amawi Firas B. 
Published: April 2022

BackgroundA lack of knowledge on adult vaccination has been documented among physicians. They play a critical role in promoting adult vaccines. This study aimed to review the status of adult vaccination in the United Arab Emirates (UAE) and evaluate physicians' knowledge and knowledge sources regarding adult vaccines.MethodsLocal, regional, and global adult vaccination guidelines were reviewed. A 40-item questionnaire was used to collect data from physicians from June to October 2020, using convenience and snowball sampling. Knowledge score was calculated, and predictors identified using Mann–Whitney U and Kruskal–Wallis H-tests. Ordinary Least Squares regression was used for Multivariate Analysis.ResultsA total of 500 responses were included. A quarter were internists, and another quarter were family physicians. Fifty-seven percent were medical interns and residents. Both perceived and actual knowledge of adult vaccination were low. Bivariate analysis showed knowledge depending on department, level of training, workplace, and perceived knowledge. All remained significant after multivariable regression except workplace. International and local guidelines were the most common knowledge sources. Forty-two percent were unable to access the local guidelines.ConclusionsPhysicians' knowledge was poor and local guidelines were not clear or easily accessible. Participants were highly receptive to guidance and practice with adult vaccines.
Mixed Reality Technology in Total Knee Arthroplasty: An Updated Review With a Preliminary Case Report
Authors: Su Shilong , Lei Pengfei , Wang Chenggong , Gao Fawei , Zhong Da , Hu Yihe 
Published: April 2022

BackgroundAugmented reality and mixed reality have been used to help surgeons perform complex surgeries. With the development of technology, mixed reality (MR) technology has been used to improve the success rate of complex hip arthroplasty due to its unique advantages. At present, there are few reports on the application of MR technology in total knee arthroplasty. We presented a case of total knee arthroplasty with the help of mixed reality technology.Case PresentationWe presented a case of a 71-year-old woman who was diagnosed with bilateral knee osteoarthritis with varus deformity, especially on the right side. After admission, the right total knee arthroplasty was performed with the assistance of MR technology. Before the operation, the three-dimensional virtual model of the knee joint of the patient was reconstructed for condition analysis, operation plan formulation, and operation simulation. During the operation, the three-dimensional virtual images of the femur and tibia coincided with the real body of the patient, showing the osteotomy plane designed before the operation, which can accurately guide the completion of osteotomy and prosthesis implantation.ConclusionsAs far as we know, this is the first report on total knee arthroplasty under the guidance of mixed reality technology.
Involvement of MiRNA-211-5p and Arhgap11a Interaction During Osteogenic Differentiation of MC3T3-E1 Cells
Authors: Ju Wenwen , Zhang Guangfeng , Zhang Xu , Wang Jingting , Wu Tong , Li Huafeng 
Published: April 2022

ObjectiveMicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells.MethodsAn MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors.ResultsWe identified 715 upregulated DEGs and 603 downregulated DEGs between MC3T3-E1 cells with and without osteoblast induction by analyzing the raw data of the GSE46400 dataset. There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. The RT-qPCR also determined declined expressions of miR-204-5p and miR-24-3p concomitant with elevated expressions of miR-211-5p, miR-3470b, miR-466b-3p, and miR-466c-3p in the MC3T3-E1 cells, with osteoblast induction compared with undifferentiated MC3T3-E1 cells. Dual-luciferase reporter gene assays demonstrated Arhgap11a as the target of miR-211-5p. MiR-211-5p upregulation by its mimic increased Arhgap11a expression in MC3T3-E1 cells.ConclusionOur study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair.
The Transumbilical Laparoendoscopic Single-Site Extraperitoneal Approach for Pelvic and Para-Aortic Lymphadenectomy: A Technique Note and Feasibility Study
Authors: Peng Shiyi , Zheng Ying , Yang Fan , Wang Kana , Chen Sijing , Wang Yawen 
Published: April 2022

BackgroundNowadays, lymphadenectomy could be performed by the transperitoneal or extraperitoneal approach. Nevertheless, each approach has its own advantages and disadvantages. Under these circumstances, we developed a transumbilical laparoendoscopic single-site (TU-LESS) extraperitoneal approach for lymphadenectomy. In this research, the primary goal is to demonstrate the feasibility of the novel approach in systematic lymphadenectomy and present the surgical process step-by-step.MethodsBetween May 2020 and June 2021, patients who had the indications of systematic lymphadenectomy underwent lymphadenectomy via the TU-LESS extraperitoneal approach. This new approach was described in detail, and the clinical characteristics and surgical outcomes were collected and analyzed.ResultsEight patients with gynecological carcinoma were included in the research, including four with high-risk endometrial cancer and four with early-stage ovarian cancer. The TU-LESS extraperitoneal approach for pelvic and para-aortic lymphadenectomy was successfully performed in all patients without conversion. In all, a median of 26.5 pelvic lymph nodes (range 18–35) and 18.0 para-aortic lymph nodes (range 7–43) were retrieved. There was a median of 166.5 min of surgical time (range 123–205). Patients had speedy recoveries without complications. All patients had positive pain responses after surgery, as well as satisfactory cosmetic and body image outcomes.ConclusionOur initial experience showed that it is feasible to perform systematic lymphadenectomy with the TU-LESS extraperitoneal approach. And this new approach may provide a new measure or a beneficial supplement for lymphadenectomy in gynecologic cancer.
Diffusion-Weighted Magnetic Resonance Imaging of Normal Lymph Nodes
Authors: Хоружик С.А. 
Published: April 2022

Цель исследования определить характеристики нормальных лимфатических узлов (ЛУ) при диффузионно-взвешенном МРТ-исследовании (ДВИ). ДВИ выполнено 92 пациентам без установленного диагноза злокачественной опухоли или других заболеваний, сопровождающихся поражением ЛУ. ЛУ шеи, подмышечные, брюшной полости, подвздошные и паховые визуализировались на изображениях с высоким фактором диффузии у всех пациентов, средостения у 28 пациентов, корней легких только у 20. Средние размеры ЛУ по короткой оси составили 78 мм независимо от анатомической группы, по длинной оси варьировали от 10 мм (ЛУ корней легких) до 14 мм (ЛУ шеи и подвздошные). Максимальный размер по короткой оси достигал 11 мм, по длинной 24 мм. Выявлена значительная вариабельность значений измеряемого коэффициента диффузии (ИКД) нормальных ЛУ, который в порядке увеличения составил (103 мм2/с): ЛУ шеи 1,250,19, подвздошные 1,250,21, подмышечные 1,460,26, паховые 1,630,26, брюшной полости 2,030,38, средостения 2,360,56, корней легких 2,550,43. При визуальной оценке 97 шейных и 61 подвздошных ЛУ имели сигнал на картах ИКД ниже сигнала мышц, большинство ЛУ остальных анатомических групп равный или выше сигнала мышц. Выявленные особенности могут быть использованы для оптимизации критериев дифференциальной диагностики нормальных и пораженных при лимфоме ЛУ.
The aim of this study is to define the characteristics of normal lymph nodes (LN) on diffusion- weighted MRI (DWI). DWI was performed in 92 patients without the known diagnosis of cancer or other diseases associated with LN involvement. The neck, axillary, abdominal, iliac and inguinal LN were visualized on high diffusion factor images in all patients, mediastinal in 28 of patients, lung hilum in 20 of patients. The average short axis diameter of LN was 78 mm, regardless of the anatomical group long axis diameter ranged from 10 mm (hilar LN) to 14 mm (neck and iliac LN). The maximum short axis diameter reached 11 mm and long axis diameter reached 24 mm. Considerable variation of apparent diffusion coefficient (ADC) in normal LN was observed, which was the following in the increasing order (103 mm2/s): neck LN 1,250,19, iliac 1,250,21, axillary 1,460,26, inguinal 1,630,26, abdominal 2,030,38, mediastinal 2,360,56, hilar 2,550,43. In visual assessment, 97 of neck LNs and 61 of iliac LN had signal below signal of muscles on ADC maps. Most of LN of other anatomical groups had the signal equal to or higher than that of muscles. The identified features can be used to optimize the criteria for the differential diagnostics of normal LN and those involved in lymphoma.
Tumor Heterogeneity and Post-Irradiation Dysfunction of Endothelium in the Assessment of Response to Treatment in Neuro-Oncology: View of the Problem and Our Practical Experience
Authors: Рудица В.И. , Бондарчук Т.И. , Робак К.О. , Ялынская Т.А. , Земскова О.В. , Главацкий А.Я. 
Published: April 2022

Оценка ответа на лечение в нейроонкологии представляет собой сложную задачу, решение которой не всегда возможно даже с помощью наиболее современных методов визуализации. В последнее время наблюдается значительный прогресс в разработке персонифицированных подходов к лечению нейроонкологических пациентов, что требует усовершенствования оценки ответа на лечение. Радиомика является новаторским направлением в диагностических технологиях и основана на применении искусственного интеллекта. К настоящему моменту такие технологии находятся на стадии ранней разработки и крайне ограниченно используются в клинической практике, в то время как перфузионные исследования, позволяющие оценить степень васкуляризации опухоли, получили широкое практическое применение. Несмотря на несомненный прогресс технологических аспектов нейровизуализации, дифференциальная диагностика псевдопрогрессии и опухолевого роста остается краеугольным камнем современной нейроонкологии. Понимание патофизиологических механизмов постлучевых реакций в эндотелии сосудов, углубление знаний относительно биологии опухолей, включая опухолевую гетерогенность, являются необходимым базисом для радиологов, которые занимаются визуализационной оценкой ответа опухоли на лечение. Данная статья демонстрирует взгляд авторов на проблему и собственный практический опыт применения МРТ-перфузии в оценке ответа на лечение в нейроонкологии.
Assessment of response in neuro-oncology is a challenge. The solution isnt easy even with the latest modern visualizing techniques. Recently, much progress has been seen in personalized approaches to the treatment of brain tumors. This demands the improvement of assessment of response to treatment. Radiomics is an emerging field of research, which aims to collect high-dimensional data from clinical images. Nowadays, such technologies are on their early stages of development, and they are limited to use in clinical practice, while perfusion imaging is widely used in clinical practice. In spite of the technological development in neuroradiology, differential diagnostics of tumor progression and pseudoprogression is still a challenge. Understanding the pathological mechanisms of post-irradiation reactions in the endothelium of vessels, increasing knowledge of tumor biology, including tumor heterogeneity are an essential ground for radiologists in assessment of response. This article demonstrates the authors opinion on the problem and their practical experience in the use of MRI perfusion in the assessment of response in neuro-oncology.
Transpupillary Thermotherapy in Treatment of Retinoblastoma
Authors: Макаревич О.О. 
Published: April 2022

В последние десятилетия акцент в менеджменте пациентов, страдающих ретинобластомой, сместился с частого использования дистанционной лучевой терапии к широкому применению цитостатических препаратов и локальных методов лечения. Данная стратегия реализуется с целью уменьшения возникновения вторых злокачественных опухолей у данной категории пациентов, особенно это актуально при наличии наследственной ретинобластомы. В распоряжении специалистов, занимающихся лечением ретинобластомы, есть такие методы лечения, как системная и интраартериальная химиотерапия, криодеструкция опухолей, лазерная коагуляция, транспупиллярная термотерапия и др. Термотерапия метод подведения тепловой энергии к тканям для локального повышения температуры, достигающей цитотоксического уровня, что в свою очередь приводит к разрушению области, которая подвергается облучению. Термотерапия может реализовываться посредством различных приборов, в том числе ультразвуковых, микроволновых, инфракрасных устройств. Лазер-индуцированная термотерапия это минимально инвазивный метод лечения опухолей различных локализаций. Транспупиллярная термотерапия это метод гипертермии посредством использования инфракрасного излучения диодного лазера. Данный метод особенно эффективен в лечении ретинобластом небольших размеров. Однако до настоящего времени нет единых алгоритмов применения данного метода у пациентов с ретинобластомой.
The emphasis in the management of retinoblastoma has shifted in recent years from the frequent use of external beam radiation therapy to the widespread implementation of chemoreduction and focal therapy. This treatment strategy has been used as a way to decrease the incidence of second malignancies in children with germinal retinoblastoma. Specialists use various techniques, including systemic and periocular chemotherapy, intraarterial chemotherapy, plaque radiotherapy, laser photocoagulation, cryotherapy, and thermotherapy. Thermotherapy is a method of delivering heat to tissue to raise local temperature to cytotoxic levels, leading to tissue death. Thermotherapy can be generated with ultrasound, infrared radiation, microwave devices, and other methods. Laser-induced interstitial thermotherapy is a minimally invasive treatment for tumors in the brain, prostate, liver, and eye. Transpupillary thermotherapy is a specific form of ocular thermotherapy using infrared radiation (diode laser). It has been the most effective for small retinoblastomas. However, there are no common techniques for this method.
Chlamydial Pneumonia in Children
Authors: Романовская О.Ф. , Романова О.Н. 
Published: April 2022

Представлен обзор литературы по этиологии, эпидемиологии, патогенезу, клиническим проявлениям, диагностике и лечению хламидийной пневмонии у детей.
The article is dedicated to a review of the etiology, epidemiology, pathogenesis, clinical manifestations, diagnosis and treatment of chlamydial pneumonia in children based on the сontemporary literature data.
Generalized Infection Сaused by Yersinia Enterocolitica in an Adolescent: a Case Report
Authors: Романовская О.Ф. , Адамович О.Л. , Романова О.Н. 
Published: April 2022

Иерсиниозная инфекция встречается повсеместно и остается третьей наиболее распространенной причиной бактериального зооноза пищевого происхождения в Европе. Клиническая картина иерсиниозной инфекции очень разнообразна. Иерсиниоз может проявляться неспецифическими симптомами, напоминающими другие заболевания: острую ревматическую лихорадку, системные заболевания соединительной ткани, туберкулез, злокачественные новообразования и др. В ряде случаев остается нераспознанным. Описан клинический случай генерализованной формы иерсиниозной инфекции у подростка, сопровождавшейся выраженным интоксикационным и нейромиалгическим синдромом при минимальных локальных проявлениях. Представлены результаты лабораторных и инструментальных методов обследования пациента в динамике.
Yersinia infection is ubiquitous and remains the third most common cause of foodborne bacterial zoonosis in Europe. The clinical presentation of Yersinia infection is very diverse. Yersiniosis can manifest with non-specific symptoms resembling other diseases: acute rheumatic fever, systemic connective tissue diseases, tuberculosis, malignant neoplasms, etc. In some cases it remains unrecognized. The case report of the generalized form of Yersinia infection in an adolescent, accompanied by prominent intoxication and neuromialgic syndrome with minimal local manifestations is described. The results of laboratory and instrumental examination of the patient in the course of disease are presented.
Measles in a Vaccinated Patient
Authors: Ластовка И.Н. , Артемчик Т.А. , Трубчик О.Н. 
Published: April 2022

Корь высококонтагиозное, острое вирусное заболевание, которое может привести к та- ким осложнениям, как пневмония, энцефалит, вплоть до летального исхода. В результате вы- сокого охвата 2-дозной вакцинацией против кори в нашем регионе сохраняется относитель- но благоприятная эпидемиологическая ситуация. Регистрация кори в Республике Беларусь связана с завозными случаями из других стран, где корь остается эндемичной. Не исключены в данной ситуации и случаи возникновения кори у иммунизированных пациентов. В статье представлен клинический случай заболевания корью у вакцинированного пациента.
Measles is a highly contagious, acute viral illness that can lead to complications such as pneumonia, encephalitis, and death. As a result of high 2-dose measles vaccination coverage in the Belarus and the high control of measles in our region, a relatively favorable measles situation remains. Nevertheless, the import of cases from other countries where measles remains endemic continues to occur, which leads to the emergence of groups of cases in Belarus. Cases of measles, even in vaccinated patients, are not excluded in this situation. The article presents a clinical case of measles in a vaccinated patient.
Ixodic Tick-Borne Borreliosis: Problems of Clinical Diagnostics in Children
Authors: Довнар-Запольская О.Н. , Чижевская И.Д. , Шеремет А.Н. 
Published: April 2022

В настоящее время, несмотря на кажущуюся простоту диагностики иксодового клещевого боррелиоза, имеются определенные трудности при постановке диагноза. Это связано прежде всего с полиморфизмом клинических проявлений болезни Лайма. В статье представлены осо- бенности клиники различных стадий иксодового клещевого боррелиоза у детей, представляющих наибольшие трудности для педиатров.
Currently, there are still some difficulties of establishing the diagnosis of ixodid tick-borne borreliosis in children. This is due to polymorphism of the clinical manifestations of Lyme disease. The article introduces some particularities of various clinical stages of ixodic tick-borne borreliosis in children, which present the most difficulties for pediatricians
Some Results of Studying the Efficiency of Vaccination Against Hepatitis B in the Republic of Belarus
Authors: Высоцкая В.С. , Коломиец Н.Д. , Глинская И.Н. , Василевская А.Е. , Романова О.Н. 
Published: April 2022

Цель. Предварительное изучение распространенности маркеров гепатита В и эффективности вакцинации против вируса гепатита В (hepatitis B virus – HBV) в отдельных группах условно здорового населения Республики Беларусь.Материалы и методы. Проведены оценка наличия протективного иммунного ответа к HBV путем определения антител к его поверхностному белку HBs-антигену (anti-HBs), а также исследование на наличие суммарных антител к HB-core антигену HBV (anti-HBc) как косвенного маркера контакта пациента с вирусом. Серопротективной концентрацией anti-HBs в сыворотке крови считали 10 мМЕ/мл и выше (ИФА, тестсистемы производства АО «Вектор-Бест» (Российская Федерация)).Результаты. Полный курс вакцинации против гепатита В обеспечивает формирование протективного иммунитета у 84,38% обследованных лиц. Доля серопозитивных лиц сокращается с увеличением времени, прошедшего после завершения вакцинации. Результаты исследования демонстрируют наличие 19,61% серонегативных лиц из числа обследованных через 10 и более лет после последней введенной дозы вакцины. Обследование 288 лиц с отрицательным результатом наличия HBsAg выявило 52 человек – 18,06%, имеющих anti-HBc.Заключение. В целях оценки и контроля эффективности проводимой вакцинации против HBV целесообразны формирование и внедрение вакцинального серологического аудита в группах риска и оптимизация тактики вакцинации взрослых лиц.
Purpose. Preliminary study of the prevalence of hepatitis B markers and the effectiveness of hepatitis virus (HBV) vaccination in certain groups of conditionally healthy population of the Republic of Belarus.Materials and methods. The presence of a protective immune response to hepatitis B virus was evaluated by determination of antibodies to the surface antigen of HB virus (anti-HBs), as well as a study for the presence of total antibodies to the HB-core antigen of HB virus (anti-HBc), as an indirect marker of patient’s contact with HB virus. Seroprotective concentration of specific antibodies in blood serum was considered to be 10 mIU/ml and higher (IFA, test systems manufactured by Vector Best JSC (Russian Federation)).Results. The full course of hepatitis B vaccination ensures the formation of protective immunity in 84.38% of the examined persons. The proportion of seropositive individuals decreases with the increase in the time elapsed after the completion of vaccination. The study results demonstrate the presence of 19.61% of seronegative individuals examined 10 years or more after the last vaccine dose administered. A survey of 288 people with a negative result of HBsAg revealed 52 people – 18.06% with anti-HBc.Conclusion. In order to assess and monitor the effectiveness of vaccinations against HV, it is advisable to form and implement a vaccine serological audit in "risk groups" and optimize the vaccination tactics of adults.