bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rieske FeS overexpression in tobacco provides increased abundance and activity of cytochrome b6f
Authors: Heyno Eiri , Ermakova Maria , Lopez‐Calcagno Patricia E. , Woodford Russell , Brown Kenny L. , Matthews Jack S. A. , Osmond Barry , Raines Christine A. , von Caemmerer Susanne 
Published: October 2022

AbstractPhotosynthesis is fundamental for plant growth and yield. The cytochrome b6f complex catalyses a rate‐limiting step in thylakoid electron transport and therefore represents an important point of regulation of photosynthesis. Here we show that overexpression of a single core subunit of cytochrome b6f, the Rieske FeS protein, led to up to a 40% increase in the abundance of the complex in Nicotiana tabacum (tobacco) and was accompanied by an enhanced in vitro cytochrome f activity, indicating a full functionality of the complex. Analysis of transgenic plants overexpressing Rieske FeS by the light‐induced fluorescence transients technique revealed a more oxidised primary quinone acceptor of photosystem II (QA) and plastoquinone pool and faster electron transport from the plastoquinone pool to photosystem I upon changes in irradiance, compared to control plants. A faster establishment of qE, the energy‐dependent component of nonphotochemical quenching, in transgenic plants suggests a more rapid buildup of the transmembrane proton gradient, also supporting the increased in vivo cytochrome b6f activity. However, there was no consistent increase in steady‐state rates of electron transport or CO2 assimilation in plants overexpressing Rieske FeS grown in either laboratory conditions or field trials, suggesting that the in vivo activity of the complex was only transiently increased upon changes in irradiance. Our results show that overexpression of Rieske FeS in tobacco enhances the abundance of functional cytochrome b6f and may have the potential to increase plant productivity if combined with other traits.
Acute haemolytic transfusion reaction after transfusion of fresh frozen plasma in a neonate—Preventable by using solvent/detergent‐treated pooled plasma?
Authors: Henricks Linda M. , Huisman Elise J. , Lopriore Enrico , Luken Jessie S. , de Haas Masja , Ootjers Claudia S. , Albersen Arjan 
Published: October 2022

AbstractBackgroundPlasma is a commonly used blood product and is available in the form of fresh frozen plasma (FFP) or pooled solvent/detergent‐treated plasma. In the Netherlands, solvent/detergent‐treated plasma has become the standard product in the adult population since several years, but for neonatal use, FFP remains the product of preference.DescriptionA preterm neonate developed lung bleeding at day 8 postpartum, for which intubation and mechanical ventilation was required and transfusions with packed red blood cells and plasma, in the form of FFP, were given. Five hours after transfusion, a red discoloration of the urine occurred. An acute haemolytic transfusion was suspected, confirmed by laboratory investigations (fast decrease in haemoglobin, increased free haemoglobin, decreased haptoglobin, increased lactate dehydrogenase and a positive direct antiglobulin test [IgG 2+]). Additional research showed that the FFP product contained nonspecific auto‐antibodies that reacted with the transfused erythrocytes, most test erythrocytes and the donor's own erythrocytes.ConclusionA neonate experienced an acute haemolytic reaction, most probably caused by administrating a FFP product containing auto‐antibodies. If transfused with solvent/detergent‐treated plasma, such antibodies would have been diluted or captured. This case adds a new argument to the discussion on expanding the use of solvent/detergent‐treated plasma to the paediatric population.
Quantifying the Value of Stochastic Supervisory Controller for Building Thermal Energy Storage Aggregators in Two-Settlement Grid Markets
Authors: Yu Min Gyung , Pavlak Gregory S. 
Published: October 2022

Abstract
Smart cities will need collections of buildings that are responsive to the variation in renewable energy generation. However, an unprecedented level of renewable energy being added to the power grid compounds the level of uncertainties in making decisions for reliable grid operation. Making autonomous decisions regarding demand management requires consideration of uncertainty in the information available for planning and executing operations. Thus, this paper aims to quantitatively analyze the performance of supervisory controllers for multiple grid-integrative buildings with thermal energy storage depending on the quality of information available. Day-ahead planning and real-time model predictive controllers were developed and compared across 50 validation scenarios when given perfect information, deterministic forecasts, and stochastic forecasts. Despite the relatively large uncertainty in the stochastic forecasts, marked improvements were observed when a stochastic optimization was solved for both the day-ahead and real-time problems. This observation underscores the need for continued development in the area of stochastic control and decision-making for future grid-interactive buildings and improved energy management of smart cities.
Do newborn infants exhale through the CPAP system? Secondary analysis of a randomised cross-over trial
Authors: Gunnarsdottir Kolbrun , Falk Markus , Baldursdottir Sonja , Donaldsson Snorri , Jonsson Baldvin , Drevhammar Thomas 
Published: October 2022

BackgroundDuring nasal continuous positive airway pressure (nCPAP) treatment in neonates, leakage is inevitable and can lead to reduced distending pressure in the lungs of the infant. In current practice, neither leakage nor expiratory flow is measured, which makes it difficult to assess if exhalation is through the device or entirely through leakages.ObjectiveTo examine if infants treated with nCPAP exhale through the CPAP system.Design and settingSecondary data analyses from the ToNIL trial on leakages during nCPAP treatment. We retrospectively examined respiratory curves for the 50 infants included in the trial, using NI LabVIEW 2015. Each infant was measured with both prongs and nasal masks. A flow recording was classified as exhalation through the system if more than 50% of all expirations showed reverse flow, each for a minimum duration of 0.1 s.Patients50 infants were included, born with a mean gestational age (GA) of 34 weeks, median birth weight of 1948 g and mean age at measurement 6.5 days. Inclusion criteria were CPAP treatment and a postmenstrual age (PMA) of 28–42 weeks.ResultsIn our measurements, 32/50 infants exhaled through the CPAP system in at least one recording with either nasal mask or prongs. Leakages exceeding 0.3 L/min were seen in 97/100 recordings.ConclusionsDuring nCPAP treatment, infants can exhale through the CPAP system and leakage was common. Measuring expiratory flows and leakages in clinical settings could be valuable in optimising CPAP treatment of infants.Trial registration numberNCT03586856.
Association of in-competition injury risk and the degree of rapid weight cutting prior to competition in division I collegiate wrestlers
Authors: Hammer Erin , Sanfilippo Jennifer L , Johnson Gary , Hetzel Scott 
Published: October 2022

ObjectivesWeight cutting is thought to offer a competitive advantage in wrestling. Dehydration has deleterious effects on physical and cognitive function, which may increase the risk of injury. The purpose of the study was to investigate whether the degree of weight cutting was associated with injury risk.MethodsData were collected prospectively in a cohort of collegiate wrestlers over seven seasons. Changes in weight, body fat and lean mass were measured during the preseason, at midseason and before competition. Cox proportional-hazard ratios were calculated for risk of in-competition injury.ResultsAmong 67 unique division 1 collegiate wrestlers (163 athlete seasons), there were 53 unique injuries affecting 46 athletes. There was no difference in absolute weight change, per cent weight change, per cent body fat change or per cent lean mass change between injured and non-injured wrestlers from the preseason to midseason measurements. From midseason to competition weight, change in body weight was −7.0%±3.2% (−5.3 kg±2.6) in injured athletes compared with −5.7%±3.3% (−4.3 kg±2.5) in non-injured athletes. For every kilogram of body weight lost, wrestlers had a 14% increased hazard of injury (HR 1.14, 95% CI 1.04 to 1.25, p=0.004). For every 1% of body weight lost, wrestlers had an 11% increased hazard of injury (HR 1.11, 95% CI 1.03 to 1.19, p=0.005).ConclusionRapid weight cutting was associated with a higher risk of in-competition injuries in division 1 collegiate wrestlers. For every per cent in body weight lost, wrestlers had an 11% increased hazard of injury during competition.
Prediction of incident atrial fibrillation with cardiac biomarkers and left atrial volumes
Authors: Lyngbakken Magnus Nakrem , Rønningen Peter Selmer , Solberg Magnar Gangås , Berge Trygve , Brynildsen Jon , Aagaard Erika Nerdrum , Kvisvik Brede , Røsjø Helge , Steine Kjetil , Tveit Arnljot , Omland Torbjørn 
Published: October 2022

ObjectiveAtrial fibrillation is a common arrhythmia associated with risk of stroke, heart failure and death. We aimed to elucidate the associations of cardiac biomarkers, echocardiographic left atrial volumetric indices and risk of prevalent and incident atrial fibrillation in the general population.MethodsWe assessed cardiac troponin T (cTnT), N-terminal pro-B-type natriuretic peptide (NT-proBNP), growth differentiation factor 15 (GDF-15), maximum (LAVimax) and minimum (LAVimin) indexed left atrial volumes and left atrial emptying fraction (LAEF) in subjects born in 1950 participating in the prospective observational cohort, Akershus Cardiac Examination 1950 Study. The Cohorts for Heart and Ageing Research in Genomic Epidemiology for Atrial Fibrillation risk score and sex was used to adjust for residual risk of atrial fibrillation.ResultsOut of 3487 subjects, 157 (4.5%) had prevalent and 123 (3.5%) had incident atrial fibrillation. Echocardiographic left atrial volumes and cardiac biomarkers associated with prevalent atrial fibrillation, but GDF-15 was non-significant in adjusted analysis. Incident atrial fibrillation was predicted by LAVimax(adjusted HR 1.51, 95% CI 1.30 to 1.75), LAVimin(adjusted HR 1.52, 95% CI 1.35 to 1.72), LAEF (adjusted HR 1.24, 95% CI 1.04 to 1.48) and NT-proBNP (adjusted HR 1.57, 95% CI 1.32 to 1.85). cTnT and NT-proBNP provided incremental prognostic information to left atrial volumes, but GDF-15 demonstrated no prognostic value for incident atrial fibrillation.ConclusionsIn the general population, echocardiographic left atrial volumetric indices and NT-proBNP, but not cTnT and GDF-15, associate with prevalent atrial fibrillation and with risk of incident atrial fibrillation. cTnT and NT-proBNP provide incremental prognostic information to echocardiography.
Diagnosing myasthenia gravis using orthoptic measurements: assessing extraocular muscle fatiguability
Authors: Keene Kevin R , de Nie Johan M , Brink Mechteld J , Notting Irene C , Verschuuren Jan J G M , Kan Hermien E , Beenakker Jan-Willem M , Tannemaat Martijn R 
Published: October 2022

IntroductionDiagnosing ocular myasthenia gravis (MG) can be challenging because serum antibodies are often not detected. We aimed to explore whether determining extraocular muscle (EOM) weakness using orthoptic measures, including an adapted Hess chart examination, can aid in diagnosing MG.MethodsWe conducted a prospective study among patients with acetylcholine receptor antibody positive MG (20 recently diagnosed, 19 chronic) and 14 seronegative MG patients. We compared orthoptic measures to 19 healthy and 18 disease controls with Graves orbitopathy, chronic progressive external ophthalmoplegia or oculopharyngeal muscular dystrophy. Maximal eye duction angles were measured using a synoptophore. Gaze deviations between eyes were measured using standard Hess chart examination with addition of 1 min persistent gaze to assess MG-associated fatiguability. Receiver operating characteristics curve analysis was performed.ResultsFor duction angles, the area under the curve (AUC) was 0.73 comparing MG to healthy, and 0.69 comparing to patient controls. For the outer field of the Hess chart, the AUC was 0.89 comparing to healthy and 0.54 to patient controls. For drift, the AUC was 0.93 comparing to healthy and 0.93 to patient controls. The sensitivity and specificity of the presence of drift was 81% and 100%.DiscussionOrthoptic measurements can be used to diagnose MG by quantifying EOM weakness and fatiguability. Drift during persistent gaze on a Hess chart is specific for MG and could be used for diagnostic purposes. The Hess chart examination is widely available, inexpensive and fast. Moreover, orthoptic measurements may be a clinically relevant outcome measure for clinical trials.
High frequency ofHTRA1ANDABCC6mutations in Japanese patients with adult-onset cerebral small vessel disease
Authors: Uemura Masahiro , Hatano Yuya , Nozaki Hiroaki , Ando Shoichiro , Kondo Hajime , Hanazono Akira , Iwanaga Akira , Murota Hiroyuki , Osakada Yosuke , Osaki Masato , Kanazawa Masato , Kanai Mitsuyasu , Shibata Yoko , Saika Reiko , Miyatake Tadashi , Aizawa Hitoshi , Ikeuchi Takeshi , Tomimoto Hidekazu , Mizuta Ikuko , Mizuno Toshiki , Ishihara Tomohiko , Onodera Osamu 
Published: October 2022

BackgroundThis study aimed to clarify the frequency and clinical features of monogenic cerebral small vessel disease (mgCSVD) among patients with adult-onset severe CSVD in Japan.MethodsThis study included patients with adult-onset severe CSVD with an age of onset ≤55 years (group 1) or >55 years and with a positive family history (group 2). After conducting conventional genetic tests forNOTCH3andHTRA1, whole-exome sequencing was performed on undiagnosed patients. Patients were divided into two groups according to the results of the genetic tests: monogenic and undetermined. The clinical and imaging features were compared between the two groups.ResultsGroup 1 and group 2 included 75 and 31 patients, respectively. In total, 30 patients hadNOTCH3mutations, 11 patients hadHTRA1mutations, 6 patients hadABCC6mutations, 1 patient had aTREX1mutation, 1 patient had aCOL4A1mutation and 1 patient had aCOL4A2mutation. The total frequency of mutations inNOTCH3,HTRA1andABCC6was 94.0% in patients with mgCSVD. In group 1, the frequency of a family history of first relatives, hypertension and multiple lacunar infarctions (LIs) differed significantly between the two groups (monogenic vs undetermined; family history of first relatives, 61.0% vs 25.0%, p=0.0015; hypertension, 34.1% vs 63.9%, p=0.0092; multiple LIs, 87.8% vs 63.9%, p=0.0134).ConclusionsMore than 90% of mgCSVDs were diagnosed by screening forNOTCH3,HTRA1andABCC6. The target sequences for these three genes may efficiently diagnose mgCSVD in Japanese patients.
NNLO interpolation grids for jet production at the LHC
Authors: Britzger D. , Gehrmann-De Ridder A. , Gehrmann T. , Glover E. W. N. , Gwenlan C. , Huss A. , Pires J. , Rabbertz K. , Savoiu D. , Sutton M. R. , Stark J. 
Published: October 2022

AbstractFast interpolation-grid frameworks facilitate an efficient and flexible evaluation of higher-order predictions for any choice of parton distribution functions or value of the strong coupling $$\alpha _{\mathrm {s}}$$

α
s

. They constitute an essential tool for the extraction of parton distribution functions and Standard Model parameters, as well as studies of the dependence of cross sections on the renormalisation and factorisation scales. The APPLfast project provides a generic interface between the parton-level Monte Carlo generator "Image missing" and both the APPLgrid and the fastNLO libraries for the grid interpolation. The extension of the project to include hadron–hadron collider processes at next-to-next-to-leading order in perturbative QCD is presented, together with an application for jet production at the LHC.
EARLY ACTIVE MOBILIZATION AFTER RECONSTRUCTION OF RUPTURED FINGER EXTENSOR TENDONS AT THE WRIST JOINT
Authors: Minami Akio , Kamiya Yukinobu , Tojo Yasuaki , Harmon Satoko Matsumoto , Sakai Akinori 
Published: October 2022

Background: Subcutaneous spontaneous ruptures of the finger extensor tendons at the wrist frequently occur. This study aims to evaluate the outcomes of patients with extensor tendon ruptures treated by our operative methods and postoperative early active mobilization. Methods: A total of 38 patients with 68 extensor tendon ruptures were included in this study. In the reconstruction of extensor tendon ruptures, tendon transfers (extensor indicis proprius (EIP) tendon transfer and/or interlacing end-to-side suture) and/or free tendon bridge graftings were performed. Immediately after operation in all patients, early active mobilization began by wearing a specially designed bandage or splint. Results: There was no reoccurrence of re-rupture of a tendon post surgery. A patient satisfaction survey revealed that 29 patients rated their results as “excellent” and 9 were “good.” Postoperatively, the active range of motion of the finger metacarpophalangeal (MP) joint averaged +3∘(range:- 14[Formula: see text]+20∘) in extension and 69∘(range: 60–80∘) in flexion. Conclusions: We treated finger extensor tendon ruptures by tendon transfer (EIP tendon transfer to the ruptured extensor tendon and/or interlacing end-to-side suture) and/or bridge tendon grafting. We employed early active mobilization with patients wearing a specially designed bandage or splint immediately after reconstructing surgery. There was no case with re-rupture postoperatively. Our operative techniques and postoperative physiotherapy as early active mobilization in this study led to excellent results after finger extensor tendon ruptures.
TIBIALIS ANTERIOR TENDON RUPTURE – EVALUATION AND FOLLOW-UP BY ULTRASONOGRAPHY
Authors: Blankstein Alexander 
Published: October 2022

Objective: The purpose of this study was to demonstrate the sonographic appearance of acute spontaneous anterior tibialis tendon (ATT) rupture, to describe the later sonographic appearance of ATT after 4–6–8 weeks of conservative treatment, and to describe the clinical outcome of these patients. Methods: This was a retrospective study of seven patients, physically active men, aged 40–75, who presented with clinical symptoms suggesting ATT rupture. All patients were treated conservatively by case immobilization and went through a follow-up ultrasound examination 4–6–8 weeks afterwards, until clinical recovery. Two patients had ultrasound examination one year after the injury. Results: All seven patients had a complete tear of the ATT with a visible gap between the torn tendon ends seen on sonography. The mean gap size at the time of initial examination was 2 cm. There was a disruption of the normal fibrillar pattern of the tendon, with non-clear fluid accumulated in the gap between the torn tendon ends. Using real-time dynamic ultrasound revealed paradoxical movement with retraction of the torn tendon ends and increasing the distance between the torn ends. All patients were treated conservatively. Sonographic follow-up examinations after 4, 6, 8 weeks revealed restoration of the tendon continuity with good active and passive movements. All seven patients demonstrated irregular and thickened tendon with echogenic scar. Clinically, at 6–8 weeks they had good range of motion (ROM) of the ankle enabling them to resume their normal daily activity.
Evaluation of Total Phenolic Content, HPLC Analysis, and Antioxidant Potential of Three Local Varieties of Mushroom: A Comparative Study
Authors: Bristy Anika Tabassum , Islam Tairin , Ahmed Rezwana , Hossain Jumana , Reza Hasan Mahmud , Jain Preeti 
Published: October 2022

Functional foods such as mushrooms are rich in polyphenolic compounds and secondary metabolites with health-promoting properties such as antioxidant, antimicrobial, antidiabetic and immunostimulatory effects. The present study is aimed to investigate the ethanolic extracts of three varieties of mushrooms, namely, G. lucidum, G. tropicum, and C. indica grown in Bangladesh for phenolic and flavonoid content and their antioxidant properties. Moreover, the phenolic composition of the extracts was analyzed by using the HPLC-DAD system. G. lucidum extract exhibited the highest antioxidant potential as evidenced by its lowest IC50 value in all the tested assay models (

40.44
±
2.09

μg/mL,

151.32
±
0.35

μg/mL,

137.89
±
1.85

μg/mL in DPPH, H2O2, and NO scavenging assay, respectively) along with the highest phenolic content (

81.34
±
0.68

GAE g-1 extract). G. tropicum and C. indica extracts also showed significant antioxidant properties and a good amount of phenolic content,

52.16
±
0.25

GAE g-1 extract, and 47.1 ± 0.26 GAE g-1 extract, respectively. The scavenging activity increased with the increasing concentration of extracts in all cases. The total phenolic content of the ethanolic extracts of mushroom species was highly correlated with antioxidant effects with Pearson’s correlation coefficient (

r

) values ranging from 0.8883–0.9851. The α-amylase inhibitory and antibacterial activity of G. lucidum was evaluated by using 3,5-dinitrosalicylic acid and disc diffusion method, respectively. The maximum inhibitory activity recorded against α-amylase was

70.98
±
0.042
%

at a concentration of 500 μg/mL. G. lucidum extract exhibited the highest antibacterial activity against Pseudomonas aeruginosa with

23.00
±
1.00

mm clear zone of inhibition and an MIC value of 3.5 mg/mL. The results indicate that the mushroom species tested in this study could serve as a potential source of natural antioxidants in the development of nutraceuticals and herbal drugs for the management of oxidative stress-associated diseases as well as infectious diseases.
A Possible Solution of the Cosmological Constant Problem Based on GW170817 and Planck Observations with Minimal Length Uncertainty
Authors: Diab Abdel Magied , Tawfik Abdel Nasser 
Published: October 2022

We propose generalized uncertainty principle (GUP) with an additional term of quadratic momentum motivated by string theory and black hole physics and providing a quantum mechanical framework for the minimal length uncertainty, at the Planck scale. We demonstrate that the GUP parameter,

β

0

, could be best constrained by the gravitational wave observations, GW170817 event. To determine the difference between the group velocity of graviton and that of the light, we suggest another proposal based on the modified dispersion relations (MDRs). We conclude that the upper bound of

β

0

reads ≃1060. Utilizing features of the UV/IR correspondence and the apparent similarities between GUP (including nongravitating and gravitating impacts on Heisenberg uncertainty principle) and the discrepancy between the theoretical and the observed cosmological constant

Λ

(obviously manifesting gravitational influences on the vacuum energy density), known as catastrophe of nongravitating vacuum, we suggest a possible solution for this long-standing physical problem,

Λ
≃
1

0

−
47

GeV4/ℏ3c3.