bluuebunny/crossref_metadata_2025_split huggingface dataset:
HDAC6 Inhibition Rescues Oxidative Stress-derived Neuronal Apoptosis Following Intracerebral Hemorrhage Via MDH1 Acetylation
Authors: Wang Miao , Zhou Chao , Yu Lu , Kong Delian , Ma Weijing , Lv Bingchen , Wang Yan , Wu Weifeng , Zhou Mingyue , Cui Guiyun 
Published: February 2022

Abstract
Oxidative stress is implicated in functional recovery after brain damage caused by intracerebral hemorrhage (ICH). Histone deacetylase 6 (HDAC6) plays an important role in initiation of oxidative stress. However, studies have not explored the role and mechanism of HDAC6 in mediating oxidative stress in ICH. Our study revealed HDAC6 knockout mice are resistant to ICH-induced oxidative stress and the resultant neuronal apoptosis, as analyses performed through MDA and NADPH/NADP+ assays, ROS detection and determination of expression levels apoptosis-related protein through western blot. Further mechanistic studies showed that HDAC6 binds to Malate dehydrogenase 1 (MDH1) and mediates MDH1 acetylation on lysine residues at position 121 and 298. ICH associated damaging agents reduce the level of MDH1 acetylation by promoting the interplay between HDAC6 and MDH1. Whereas, acetylation of MDH1 increases when HDAC6 is inhibited. Functional verification of acetylation MDH1 demonstrates that non-acetylation mutant MDH1, rather than its acetylation resistant mutant, protects neuron from oxidative injury. In addition, HDAC6 inhibition failed to mediate brain damage alleviation when MDH1 was knockdown. Overall, the findings of the present study indicated the protect effect of HDAC6 inhibition resistance to brain damage during ICH requires MDH1 acetylation.
Ultrafast Femtosecond Laser Maskless Patterning for Multitype Microsupercapacitors
Authors: Yuan Yongjiu , Jiang Lan , Li Xin , Liang Misheng , Xu Chenyang , Ma Le , Wu Junrui , Huang Junqi , Zhao Yang , Qu Liangti 
Published: February 2022

Abstract
Downsizing electrode architectures provides great potential for the fabrication of microscale energy storage devices. With their extended voltage window and high energy density, asymmetric microsupercapacitors (MSCs) play an essential role in various applications. However, the efficient manufacturing of asymmetric MSCs remains challenging. Herein, maskless lithography–based ultrafast fabrication of multitype micron-sized planar MSCs in one step through temporally and spatially shaped femtosecond laser is presented. MSCs are only 10 × 10 µm2 in size and have a minimum line width of 200 nm. MXene and 1T-MoS2 can be combined with laser-induced MXene-derived TiO2 and 1T-MoS2-derived MoO3 to form various types of asymmetric and symmetric MSCs in the same material system within seconds. The asymmetric MSC exhibits an ultrahigh speciଁc capacitance (220 mF cm−2 and 1101 F cm−3), cycling stability (98.3% capacitance retention after 15,000 cycles), energy density (0.495 Wh cm−3) and power density (28 kW cm−3). This versatile strategy overcomes the current limitations of MSC manufacturing. Multitype MSCs fabricated herein have high resolution, enhancing the feasibility and flexibility of the preparation of microscale energy storage devices.
Is the Local Energy Conservation Law Valid inside the Electronic Device of Charged Capacitors?
Authors: Jeong Eue-Jin 
Published: February 2022

Abstract
The energy conservation is one of the most fundamental and well established principles in physics. Emmy Noether extended the energy conservation principle to the quantum field theoretical domain in empty space by relating the time translation invariance of the universe with energy conservation. While this is the case in open empty space, it seems that the local space enclosed by conducting metallic plates has unexpected property suggesting that the energy conservation principle does not necessarily apply to localized bound system of capacitors in electrodynamics. This point of view was raised by noticing the fact that the spherical capacitor has calculable electrostatic self-potential energy in both the inner and outer shells respectively which was not taken into account in the conventional consideration of the total energy stored in the capacitors. It seems like the concept of moving charges one by one into the capacitor plates has helped bypassing the necessary steps to account for the additional repulsive self-potential energy that accumulates simultaneously in both of the capacitor plates in the process of charging the capacitor. We present the dynamics of the repulsive potential energy stored in capacitors and discuss its physical reality in relation to the anomalous energy devices reported in the past.
Genetic Profiles to Identify Talents in Elite Endurance Athletes and Professional Football Players
Authors: Varillas-Delgado David , Morencos Esther , Gutiérrez-Hellín Jorge , Aguilar-Navarro Millán , Muñoz Alejandro , Láiz Nuria Mendoza , Perucho Teresa , Maestro Antonio , Tellería-Orriols Juan José 
Published: February 2022

Abstract
Background
The genetic profile that is needed to identify talents has been studied extensively in recent years. The main objective of this investigation was to approach, for the first time, the study of genetic variants in several polygenic profiles and their role in elite endurance and professional football performance by comparing the allelic and genotypic frequencies to the non-athlete population.
Methods
Genotypic and allelic frequencies were determined in 452 subjects; 292 professional athletes (160 elite endurance athletes and 132 professional football players) and 160 non-athlete subjects. Genotyping of liver metabolism, iron metabolism and energy efficiency, cardiorespiratory fitness and muscle performance was performed by Polymerase Chain Reaction-Single Nucleotide Primer Extension (PCR-SNPE). The combination of the polymorphisms for the “optimal” polygenic profile was quantified using the genotype score (GS) and total genotype score (TGS).
Results
Statistical differences were found in the genetic distributions between professional athletes and the non-athlete population in liver metabolism, iron metabolism and energy efficiency, and muscle performance (p<0.001). The binary logistic regression model showed a favourable OR (odds ratio) of being a professional athlete against a non-athlete in liver metabolism (OR: 1.965; 95% CI: 1.285–3.016; p=0.002), iron metabolism and energy efficiency (OR: 2.213; 95% CI: 1.425-3.438; p<0.001), and muscle performance (OR: 2.700; 95% CI: 1.750-4.165; p<0.001) in the polymorphisms studied.
Conclusions
Genetic distribution in professional athletes as regards endurance (professional cyclists and elite runners) and professional football players shows genetic selection in these sports disciplines.
IGF2BP3 promotes progression of colorectal cancer and mediates cetuximab resistance by stabilizing EGFR mRNA in an m6A-dependent mechanism
Authors: Jiao Hongli , Chen Lijie , Liu Huiye , Xiao Zhiyuan , Qiu Ting , Zhang Dan , Zhang Lingjie , Han Fangyi , Chen Guojun , Xu Xuemei , Zhu Jionghua , Wang Shuyang , Ding Yanqing , Ye Yaping 
Published: February 2022

Abstract
Background: Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), an RNA-binding protein, is associated with tumorigenesis and progression. However, the exact molecular mechanisms of IGF2BP3 in colorectal cancer (CRC) oncogenesis, progression, and drug resistance remain largely unclear.Methods: Transcriptome-sequencing, bioinformatics analysis, Western blot, and immunohistochemistry were performed to evaluate the expression of IGF2BP3 in CRC. Gene enrichment analysis and qPCR were used to assess the downstream targets of IGF2BP3. The molecular mechanisms of IGF2BP3 on CRC progression were explored through RNA stability assay, m6A dot-blot, luciferase reporter assay, and MeRIP assay. In vitro and in vivo experiments were conducted to investigate the effects of IGF2BP3 on oncogenesis, progression and sensitivity to cetuximab of CRC. Results: IGF2BP3 was upregulated in CRC tissues. Clinically, elevated IGF2BP3 level was predictive of a poor prognosis. Functionally, IGF2BP3 enhanced CRC tumorigenesis and progression in vitro and in vivo. Mechanistically, IGF2BP3 promoted epidermal growth factor receptor (EGFR) mRNA stability and translation, then further activated the EGFR pathway by serving as a reader in an N6-methyladenosine (m6A)-dependent manner by cooperating with METTL14. Furthermore, IGF2BP3 increases drug resistance of CRC cells to the EGFR-targeted antibody cetuximab. Conclusions: IGF2BP3 upregulation serve as an important oncogene in the development of CRC. It stabilizes EGFR mRNA via an m6A-dependent manner and further promotes CRC progression and drug resistance to cetuximab. Targeting IGF2BP3 and m6A modification offers rational therapeutic targets in CRC patients.
Geothermal Renewable Energy Prospects of The African Continent Using GIS
Authors: Elbarbary Samah , Zaher Mohamed Abdel , Saibi Hakim , Fowler Abdel-Rahman , Saibi Kamal 
Published: February 2022

Abstract
Geothermal energy potential is identified through the deep investigation of geological, geothermal, and geophysical information over a small area; however, this task is both expensive and complex. Geographic Information Systems (GIS) can integrate different types of data (or thematic layers) over large regions and use them to identify regions of high geothermal potential. In this study, a GIS tool is used to construct the first regional-scale geothermal potential map of Africa from different datasets. The key objective of this study is to estimate the geothermally promising areas within Africa by integrating geological thematic layers (rock units and faults), geophysical layers (heat flow derived from aeromagnetic data and seismicity), and geothermal layers (hot springs and volcanoes) within the GIS database. A weighted overlay technique within the GIS environment is applied to these data to generate the geothermal potential map. The result shows 14 regions with a high geothermal favorability index. The geothermal potential map of Africa is useful for targeting and exploring new geothermal renewable energy sites and can reduce exploration costs and pinpoint investigation areas during preliminary geothermal studies.
Multifaceted Defects in Monocytes in Different Phases of Chronic HBV Infection: Persistence After Antiviral Therapy
Authors: Dey Debangana , Pal Sourina , Chakraborty Bidhan Chandra , Bhadra Soham , Ghosh Ranajoy , Banerjee Soma , Ahammed SK Mahiuddin , Chowdhury Abhijit , DATTA SIMANTI 
Published: February 2022

Abstract
Background
Monocytes play an important role in the control of microbial infection but monocyte biology during chronic HBV infection (CHI) remains inadequately studied. We investigated the frequency/phenotype/functions of monocytes in different phases of CHI namely, Immune-tolerant (IT), HBeAg-positive/HBeAg-negative (EP/EN) chronic hepatitis B (CHB) and Inactive carriers (IC), identified factors responsible for their functional alterations and determined the impact of antiviral-therapy on these cells.
Methods
Multicolor flow cytometry/cell-sorting/co-culture experiments/confocal microscopy were performed.
Results
HLA-DR+CD14++CD16− classical-monocytes were found to be significantly reduced while HLA-DR+CD14++CD16+ intermediate- and HLA-DR+CD14+CD16++ non-classical-monocyte-subsets were expanded in IT and EP-/EN-CHB than IC and healthy controls (HC). In comparison to IC/HC, monocytes (including all subsets) in IT/CHB exhibited diminished expression of TLR-2/TLR-4/TLR-9 and cytokines IL-12/TNF-α/IL-6 but produced higher levels of IL-10/TGF-β. Further, monocytes in CHB/IT showed impaired phagocytosis and oxidative response relative to IC/HC. In vitro assays indicated that high titres of hepatitis B surface antigen (HBsAg) present in IT/CHB and IL-4 in CHB triggered the functional defects in monocytes via induction of β-catenin. Additionally, monocyte-derived M1-macrophages of CHB/IT produced less pro-inflammatory and more anti-inflammatory cytokines than those of IC/HC whereas, monocytes in CHB/IT skewed the differentiation of CD4+-T-cells towards regulatory T-cell and Th2-dominated phenotype. Moreover, in CHB/IT, monocytes overexpressed chemokine-receptor CCR2, which coincided with increased intrahepatic accumulation of β-catenin+CD14+-cells. One-year of Tenofovir-therapy failed to normalize monocyte functions or reduce serum HBsAg/IL-4 levels.
Conclusions
Monocytes are functionally perturbed mostly in IT and EP-/EN-CHB phases. Targeting intramonocytic β-catenin or reducing HBsAg/IL-4 levels might restore monocyte function and facilitate viral clearance.
Necroptosis-related lncRNAs and Hepatocellular Carcinoma Undoubtedly Secret
Authors: Ma Cuiqing , Ning Jingyuan , Sun Keran , Fan Xiaoqing , Jia Keqi , Wang Xiaofeng , Wei Lin 
Published: February 2022

Abstract
Background: The current study demonstrates that necroptosis is an important mechanism of carcinogenesis. However, the predictive value of necroptosis-associated long non-coding RNAs (LncRNAs) in hepatocellular carcinoma has not been demonstrated. The purpose of this study is to apply necroptosis-related lncRNAs to construct a predictive signature to predict the prognosis of hepatocellular carcinoma patients.Methods: The clinical and RNA-seq data were downloaded using The Cancer Genome Atlas (TCGA) database. Univariate and multivariate COX analysis was used to screen out suitable necroptosis-related lncRNAs, and then predictive signature was constructed. The TCGA data were randomly divided into high- and low-risk groups, and the Kaplan-Meier method was used to analyze the overall survival (OS) of the two groups to verify the predictive signature. Finally, a prognostic correlation model for predicting disease-freesurvival （DFS）in hepatocellular carcinoma（HCC） was constructed and validated.Results: We had screened out 9 necroptosis-related lncRNAs (BACE1-AS, LINC01188, LUCAT1, PI3KCD-AS2, Z83851.1, AC009283.1, AC012360.2, AC015908.3, AC103760.1), which were used to construct a predictive signature, and draw the receiver operating characteristic (ROC) curve by the high- and low-risk group. It was found that the area under the curve (AUC) of risk score was 0.874, and the AUC values of 1-,3-,and 5-years were 0.8, 0.759, 0.787. The TCGA data were randomly divided into two cohorts. In two cohorts, The OS of the high-risk groups were significantly lower than that of the low-risk groups, and the AUC of the ROC curves of the two cohorts were 0.851, 0.804, 0.802 and 0.735, 0.716, 0.76 at 1-, 3-, and 5-years. After quantifying immune cell subsets and related functions, it was found that the infiltration of active dendritic cells (aDCs), macrophages, mast cells, natural killer cells (NK), T regulatory cells (Tregs) were significantly different, and the expressions of immune checkpoints CD86, LAIR1, CTLA4, VTCN1, TNFRSF18, CD80, CD276, HHLA2, TNFSF4, TNFRSF8, TNFRSF4, TNFRSF9, LGALS9, HAVCR2 and TNFSF15 were also significantly different.Conclusion: This predictive signature can accurately predict the prognosis of hepatocellular carcinoma patients and provide guidance for the clinical treatment of hepatocellular carcinoma patients.
Predicting survival and risk stratification based on nomogram for locally advanced cervical cancer patients of the 2018 FIGO staging system treated with radiochemotherapy in southern China: a retrospective study
Authors: Zhang Yong , Li Xinshu , Huang Lulu , Jiang Li , Ma Shanshan , Shen Meizhu , Que Yanmei , Que Jinting , Qin Nianqun , Wu Fang 
Published: February 2022

Abstract
Background: Concurrent chemoradiation has been the standard treatment for locally advanced cervical cancer patients worldwide. This study was conducted to construct the nomogram of prognosis and risk stratification for locally advanced cervical cancer in southern ChinaMaterial and Methods: The medical records of 258 LACC patients who mostly received cisplatin-based concurrent chemoradiation and high-dose-rate intracavity brachytherapy at the first affiliated hospital of Guangxi Medical University were analyzed. Stepwise selection of 22 factors with the Elastic net method was used to obtain a predictive model and construct nomograms for both progression-free survival (PFS) and overall survival (OS).Results: The median follow-up period was 47 months. The 3-year and 5-year OS were 73.7% and 65.9%, respectively, while the 3-year and 5-year PFS were 68.1% and 60.2%. In the multivariable analysis, different cumulative cisplatin dose (<180mg/m2: HR: 0.099, 95% CI: 0.010–0.909, p=0.040; 180-200mg/m2: HR: 0.058, 95% CI: 0.006-0.545, p=0.012; >200mg/m2: HR: 0.050, 95% CI: 0.006–0.520, p=0.012), bulky tumors (HR: 1.869, 95% CI: 1.127–3.098, p=0.015), ICBT (HR: 0.385, 95% CI: 0.218–0.681, p=0.001), and thrombocytopenia pre-treatment (HR: 6.832, 95% CI: 1.169–39.902, p=0.032) had significant effect on PFS, and OS was influenced by bulky tumors (HR: 1.916, 95% CI: 1.100–3.336, p=0.021), ICBT (HR: 0.499, 95% CI: 0.252-0.986, p=0.045), thrombocytopenia pre-treatment (HR: 3.865, 95% CI: 1.367-10.922, p=0.010) and high neutrophilic granulocyte percentage level in early stages of the treatment (HR: 2.041, 95% CI: 1.172–3.552, p=0.011).Nomograms of the independent predictors were established (cross-validated concordance probability c=0.671 for PFS, and 0.673 for OS). Based on the scores of the nomogram, subgroups of risk stratification further constructed showed a statistically difference in survival (p<0.05).Conclusions: Our study shows the prognostic value of nomogram to predict survival and establish risk stratification for LACC patients from southern China. Future individualized tailoring of therapy should focus even further on the high-risk patient populations.
Spatial-Temporal Evolution of ESV In The Yellow River Basin And Its Response To Land Use Change
Authors: Yang Jie , Xie Baopeng , Zhang Degang 
Published: February 2022

Abstract
Evaluating the value of ecosystem services and clarifying their temporal and spatial characteristics can effectively assist regions in formulating targeted and localized sustainable ecological management strategies. This study takes the Yellow River Basin (YRB）in China as the research area, analyzes the ecosystem service value (ESV) and its spatial-temporal variation characteristics in this region, using the equivalent factor method and geospatial exploration method, introduces the elasticity coefficient, and explores the response of ESV change to land use change, based on the land use cover data from 1990 to 2020. The results show that from 1990 to 2020, the ESV of the YRB showed an overall increasing trend, mainly because the ecological construction project increased the forest land and grassland in the region, thus the ecosystem service function was improved. In the past 28 years, the spatial characteristics of ESV in the YRB are relatively stable. The high-value areas are mainly distributed in the upper Yellow River, while the low-value areas are mainly distributed in the lower Yellow River, but the cold spots and hot spots are reduced. The ESV barycenter coordinates show the direction of the transfer trajectory, which is first to the southwest, then to the northeast, and then to the southwest. From 2000 to 2010, the land use change in the YRB had a greater impact on ESV. Since 2010, the disturbance of ecosystem services by land use change has decreased. The elastic index of the upstream region and the Loess Plateau region is significantly higher than that of other regions, and the impact of land use change on ecosystem services is more obvious, which is mainly because the large-scale implementation of ecological construction projects has significantly improved the ecological function of the region. Our results is conducive to promoting the coordination of sustainable development and ecological protection in YRB, which is based on a comprehensive spatial-temporal assessment of ESV.
What is the impact of COVID-19 pandemic years on Deliveries and Home Based New Born Care in India?
Authors: Kumar Piyush 
Published: February 2022

Abstract
Background:The COVID-19 pandemic caused by SARS-CoV-2 has led to disruption of health services globally. I assessed the effect of the ongoing pandemic on the Deliveries and Home Based New Born Care (HBNC) in India. The author is curious and suspects a rise in maternal and neonatal mortality due to possible negative effects of COVID-19 on maternal institutional delivery and HBNC. Hence author decided to find out answer to the research question mentioned as title above.Methods:I conducted a retrospective cross-sectional comparative study among total numbers of women and newborn who have received delivery and HBNC care at different health facilities in 36 states and union territories across India during the pandemic years with pre-pandemic years (comparing 2020 and 2021(up to May) with previous two years of pre-pandemic era i.e. 2018-2019). The data for the research study is extracted from electronic records of HMIS (health management information system) of MoHFW (ministry of health and family welfare), Govt. of India and analysed with stata and Microsoft office.Results:The mean number of home deliveries attended by SBA [(Skill Birth Attendant) (Doctor/Nurse/ANM/ Midwife)] during the pandemic year 2021 with total number of observation of 5 months across India was 15491, for years 2020, 2019,2018 it was 16429.25 ,19006.58 , 21317.83 respectively with total number of observation of 12 months across India for these three years. This research study revealed that pandemic years have negative impact on number of home deliveries attended by SBA [(Skill Birth Attendant) (Doctor/Nurse/ANM/ Midwife)]. This research study revealed that pandemic years have negative impact on number of home deliveries attended by SBA [(Skill Birth Attendant) (Doctor/Nurse/ANM/ Midwife)]. There is a decrease of 27.33 % in mean number of home deliveries attended by SBA in 2021 as compared to 2018.Conclusions: The ongoing COVID-19 pandemic has had negative effects on Deliveries and Home Based New Born Care (HBNC) in India. More research is required to investigate the direct and indirect consequence of the pandemic on birth and provision of HBNC as well as the health facility type which are not performing well in providing respectful maternity care and newborn services during pandemic period. The facility based research analysis is under process by the author which will be available in next version of this article.