bluuebunny/crossref_metadata_2025_split huggingface dataset:
Necroptosis-dependent Immunogenicity of Cisplatin: Implications for Enhancing the Radiation-induced Abscopal Effect
Authors: Luo Ren , Onyshchenko Kateryna , Wang Liqun , Gaedicke Simone , Grosu Anca-Ligia , Firat Elke , Niedermann Gabriele 
Published: November 2022

Abstract

Purpose:
Cisplatin is increasingly used in chemoimmunotherapy and may enhance the T cell–dependent radiation-induced abscopal effect, but how it promotes antitumor immunity is poorly understood. We investigated whether and why cisplatin is immunogenic, and the implications for the cisplatin-enhanced abscopal effect.

Experimental Design:
Cisplatin, carboplatin, and the well-known immunogenic cell death (ICD) inducer oxaliplatin were compared for their potency to enhance the abscopal effect and induce type I IFN (IFN-I) and extracellular ATP, danger signals of ICD. The hypothetical role of necroptosis and associated damage-associated molecular patterns for cisplatin-induced ICD was investigated by inhibitors and knockout cells in vitro and in two tumor models in mice. A novel necroptosis signature for tumor immune cell infiltration and therapy response was developed.

Results:
Cisplatin enhanced the abscopal effect more strongly than oxaliplatin or carboplatin. This correlated with higher induction of IFN-I and extracellular ATP by cisplatin, in a necroptosis-dependent manner. Cisplatin triggered receptor-interacting protein kinase 3 (RIPK3)-dependent tumor cell necroptosis causing cytosolic mitochondrial DNA (mtDNA) release, initiating the cyclic GMP–AMP synthase–stimulator of interferon genes pathway and IFN-I secretion promoting T-cell cross-priming by dendritic cells (DC). Accordingly, tumor cell RIPK3 or mtDNA deficiency and loss of IFN-I or ATP signaling diminished the cisplatin-enhanced abscopal effect. Cisplatin-treated tumor cells were immunogenic in vaccination experiments, depending on RIPK3 and mtDNA. In human tumor transcriptome analysis, necroptotic features correlated with abundant CD8+ T cells/DCs, sparse immunosuppressive cells, and immunotherapy response.

Conclusions:
Cisplatin induces antitumor immunity through necroptosis-mediated ICD. Our findings may help explain the benefits of cisplatin in chemo(radio)immunotherapies and develop clinical trials to investigate whether cisplatin enhances the abscopal effect in patients.
TXNIP Suppresses the Osteochondrogenic Switch of Vascular Smooth Muscle Cells in Atherosclerosis
Authors: Woo Sang-Ho , Kyung Dongsoo , Lee Seung Hyun , Park Kyu Seong , Kim Minkyu , Kim Kibyeong , Kwon Hyo-Jung , Won Young-Suk , Choi Inpyo , Park Young-Jun , Go Du-Min , Oh Jeong-Seop , Yoon Won Kee , Paik Seung Sam , Kim Ji Hyeon , Kim Yong-Hwan , Choi Jae-Hoon , Kim Dae-Yong 
Published: November 2022

Background:
The osteochondrogenic switch of vascular smooth muscle cells (VSMCs) is a pivotal cellular process in atherosclerotic calcification. However, the exact molecular mechanism of the osteochondrogenic transition of VSMCs remains to be elucidated. Here, we explore the regulatory role of TXNIP (thioredoxin-interacting protein) in the phenotypical transitioning of VSMCs toward osteochondrogenic cells responsible for atherosclerotic calcification.

Methods:

The atherosclerotic phenotypes of
Txnip
-/-
mice were analyzed in combination with single-cell RNA-sequencing. The atherosclerotic phenotypes of
Tagln
-Cre;
Txnip
flox/flox
mice (smooth muscle cell-specific
Txnip
ablation model), and the mice transplanted with the bone marrow of
Txnip
-/-
mice were analyzed. Public single-cell RNA-sequencing dataset (GSE159677) was reanalyzed to define the gene expression of TXNIP in human calcified atherosclerotic plaques. The effect of TXNIP suppression on the osteochondrogenic phenotypic changes in primary aortic VSMCs was analyzed.

Results:

Atherosclerotic lesions of
Txnip
-/-
mice presented significantly increased calcification and deposition of collagen content. Subsequent single-cell RNA-sequencing analysis identified the modulated VSMC and osteochondrogenic clusters, which were VSMC-derived populations. The osteochondrogenic cluster was markedly expanded in
Txnip
-/-
mice. The pathway analysis of the VSMC-derived cells revealed enrichment of bone- and cartilage-formation–related pathways and bone morphogenetic protein signaling in
Txnip
-/-
mice. Reanalyzing public single-cell RNA-sequencing dataset revealed that TXNIP was downregulated in the modulated VSMC and osteochondrogenic clusters of human calcified atherosclerotic lesions.
Tagln
-Cre;
Txnip
flox/flox
mice recapitulated the calcification and collagen-rich atherosclerotic phenotypes of
Txnip
-/-
mice, whereas the hematopoietic deficiency of TXNIP did not affect the lesion phenotype. Suppression of TXNIP in cultured VSMCs accelerates osteodifferentiation and upregulates bone morphogenetic protein signaling. Treatment with the bone morphogenetic protein signaling inhibitor K02288 abrogated the effect of TXNIP suppression on osteodifferentiation.

Conclusions:
Our results suggest that TXNIP is a novel regulator of atherosclerotic calcification by suppressing bone morphogenetic protein signaling to inhibit the transition of VSMCs toward an osteochondrogenic phenotype.
Morphological and molecular analyses of the six-spotted spider mite, Eotetranychus sexmaculatus (Riley) (Tetranychidae)—a pest more widespread than anticipated?
Authors: BEARD JENNIFER J. , BEAULIEU FRÉDÉRIC , KNEE WAYNE , GOTOH TETSUO 
Published: November 2022

A spider mite species initially identified as the six-spotted spider mite, Eotetranychus sexmaculatus (Riley), was recorded defoliating avocado trees, Persea americana Mill. (Lauraceae), in the southwestern parts of Western Australia. However, due to morphological inconsistencies in the descriptions of E. sexmaculatus, it has recently been suggested that these Australian specimens actually represented the native species E. queenslandicus Manson and that E. sexmaculatus was in fact not present in Australia (Seeman et al. 2017).
Exploring the diversity and host association of tarsonemid mites in West Bengal, a highly biodiverse state of Eastern India
Authors: KARMAKAR KRISHNA , MONDAL PRIYANKAR , GANGULY MOUMI , KAYAL SANDIPAN 
Published: November 2022

The family Tarsonemidae is an extremely diverse assemblage of heterostigmatid mites which include phytophagous, fungivorous, detriphagous, algivorous and insect parasitic species living in various terrestrial, arboreal, subcortical or nidicolous habitats. Many of these mites pose a serious threat to global agriculture due to their direct feeding on crop plants or indirect transmission of disease-causing bacterial or fungal spores to healthy plants. For instance, Polyphagotarsonemus latus may reproduce year-round in all tropical and subtropical habitats and feed on more than 60 different plant families. Steneotarsonemus species, such as S. spinki and S. subfurcatus, attack rice and can significantly reduce the harvest, S. ananas attacks pineapple, and S. laticeps targets lilies. In temperate forests of the Northern Hemisphere, several Tarsonemus species phoretic on curculionid beetles transfer lethal fungal spores on healthy pine trees, while some other species pose a real hazard to cultivated mushrooms and stored products.
Identification of effective insecticides to manage Petrobia latens outbreaks in winter wheat
Authors: ROYER TOM A. , HESS THOMAS , ZARRABI ALI 
Published: November 2022

Petrobia latens (Müller) is a sporadic, but important pest of winter wheat in Oklahoma. Outbreaks are associated with prevalent hot, dry conditions (Jepson et al. 1975; Dhooria 2016). Because outbreaks are sporadic, updated data on acaricide efficacy against P. latens is sparse, and growers need to know what products are available to effectively control them when outbreaks occur (Depew 1968; Henderson & Tilton 1955; Saxena & Rawat 1969). We conducted two studies in established winter wheat to evaluate selected insecticides (bifenthrin @ 0.02 kg ai/ha, dimethoate @ 0.28 kg ai/ha, gamma cyhalothrin @ 0.015 kg ai/ha, lambda cyhalothrin @ 0.03 kg ai/ha, and zeta-cypermethrin @ 0.028 kg ai/ha) for their effectiveness at controlling P. latens. The first study (field 1) was conducted in a wheat field at Feekes growth stage 3.0 (Feekes 1941), and the second in a wheat field (Field 2) at Feekes growth stage 4.0. Mite populations in Field 1 averaged 421 mites per m2 and 1694 per m2 in Field 2. Growth stage affected control, with % control ranging from 48% to 64% in Field 1, while % control ranged from 81% to 88% in Field 2.
The arthropods of corpses from above ground and from deep below
Authors: PEROTTI M. ALEJANDRA , BRAIG HENK R. 
Published: November 2022

The Acari are one of the most ubiquitous arthropod inhabitants and associates of human and animal remains. Over 150 years ago, Jean Pierre Mégnin proposed that mites arrive at corpses at two particular stages of the decomposition process, that is within the first and the sixth waves of arthropod arrival or colonisation event (Mégnin, 1895). Now we know that mites actually arrive at each stage of the decomposition process of corpses, in a continuum (Rai et al., 2021). Interestingly, the mite fauna of cadavers is very diverse, and mite species composition varies as decomposition progresses and according to the environment where decomposition occurs (Baker, 2009; Braig & Perotti, 2009). In fact, specific stages of decomposition can be characterised by the associated mite species (Kamaruzaman et al., 2018; Leclercq & Verstraeten, 1988; Mašán et al., 2013; Mégnin, 1895).
Hyper-Realistic Advanced Surgical Skills Package with Cut Suit Simulator Improves Trainee Surgeon Confidence in Operative Trauma
Authors: Chao Edward , Liveris Anna , Yusaf Tricia , Batista Gabriela , Diaz Dajelyn , Cruz Juan , Lee Alex-Sungbae , Pohlman Jessica , Andrews Kiah , Bukur Marko , Teperman Sheldon , Klein Michael J. 
Published: November 2022

Background Adequate exposure to operative trauma is not uniform across surgical residencies, and therefore it can be challenging to achieve competency during residency alone. This study introduced the Cut Suit surgical simulator with an Advanced Surgical Skills Package, which replicates traumatic bleeding and organ injury, into surgery resident training across multiple New York City trauma centers. Methods Trainees from 6 ACS-verified trauma centers participated in this prospective, observational trial. Groups of 3-5 trainees (post-graduate year 1-6) from 6 trauma centers within the largest public healthcare network in the U.S. participated. Residents were asked to perform various operative tasks including rescucitative thoracotomy, exploratory laprotomy, splenectomy, hepatorrhaphy, retroperitoneal exploration, and small bowel resection on a severely injured simulated patient. Pre- and post-course surveys were used to evaluate trainees’ confidence performing these procedures and quizzes were used to evaluate participants’ knowledge acquisition after the simulation. Results One hundred twenty-three surgery residents participated in the evaluation. 68% of participants agreed that the simulation was similar to actual surgery. After the simulation, the percentage of residents reporting being “more confident” or “most confident” in independently managing operative trauma patients increased by 42% ( P < .01). There was a significant increase in the proportion of residents reporting being “more confident” or “most confident” managing all procedures performed. Post-activity quiz scores improved by an average of 20.4 points. Discussion The Cut Suit surgical simulator with ASSP is a realistic and useful adjunct in training surgeons to manage complex operative trauma.
Osteoperiosteal decortication and bone grafting combined with wave plating for femoral shaft aseptic atrophic nonunion after intramedullary nailing
Authors: Lu Yao , Sun Liang , Wang Qian , Ren Cheng , Xu Yibo , Ye Haobo , Li Ming , Xue Hanzhong , Huang Qiang , Li Zhong , Zhang Kun , Ma Teng 
Published: November 2022

Objective Femoral shaft nonunion after intramedullary nailing fixation remains a challenge for orthopedic surgeons. This study evaluated osteoperiosteal decortication and iliac bone grafting combined with wave plating as a treatment for aseptic atrophic nonunion of the femoral shaft after intramedullary nailing. Methods The study included 22 patients (two female, 20 male; mean age 40.8 years [range, 19–61]) with aseptic atrophic nonunion of the femoral shaft who underwent osteoperiosteal decortication and iliac bone grafting combined with wave plating between January 2016 and January 2020. Cases with infected nonunion, hypertrophic nonunion, and nonunion after plate osteosynthesis were excluded. Clinical outcomes were assessed retrospectively using the Samantha X-ray and Paley scale scores. Results The mean follow-up period was 18.8 months (range, 12–35). Bone union was achieved in all patients. The average clinical healing time was 5.7 months (range, 3–14). The mean Samantha X-ray score was 5.7 ± 0.45 and the Paley scores indicated excellent or good clinical results in all cases. One patient developed sterile wound leakage that resolved after a dressing change. Conclusions Osteoperiosteal decortication and bone grafting combined with wave plating is an excellent treatment for aseptic atrophic nonunion of the femoral shaft after intramedullary nailing.
Factors Impacting Access and Engagement of Cognitive Remediation Therapy for People with Schizophrenia: A Systematic Review
Authors: Altman Rosalie Ariane Eva , Tan Eric Josiah , Rossell Susan Lee 
Published: November 2022

Objectives Neurocognitive deficits are central in schizophrenia. Cognitive remediation has proven effective in alleviating these deficits, with medium effect sizes. However, sizeable attrition rates are reported, with the reasons still uncertain. Furthermore, cognitive remediation is not part of routine mental health care. We conducted a systematic review to investigate factors that influence access and engagement of cognitive remediation in schizophrenia. Methods We systematically searched the PubMed, Web of Science, and PsycINFO databases for peer-reviewed articles including a cognitive remediation arm, access, and engagement data, and participants with schizophrenia spectrum disorders aged 17–65 years old. Duplicates and studies without a distinct cognitive remediation component, protocol papers, single case studies, case series, and reviews/meta-analyses were excluded. Results We included 67 studies that reported data on access and engagement, and extracted quantitative and qualitative data. Access data were limited, with most interventions delivered on-site, to outpatients, and in middle- to high-income countries. We found a median dropout rate of 14.29%. Only a small number of studies explored differences between dropouts and completers ( n = 5), and engagement factors ( n = 13). Dropouts had higher negative symptomatology and baseline self-efficacy, and lower baseline neurocognitive functioning and intrinsic motivation compared to completers. The engagement was positively associated with intrinsic motivation, self-efficacy, perceived usefulness, educational level, premorbid intelligence quotient, baseline neurocognitive functioning, some neurocognitive outcomes, and therapeutic alliance; and negatively associated with subjective cognitive complaints. Qualitative results showed good acceptability of cognitive remediation, with some areas for improvement. Conclusions Overall, access and engagement results are scarce and heterogeneous. Further investigations of cognitive remediation for inpatients, as well as remote delivery, are needed. Future clinical trials should systematically explore attrition and related factors. Determining influential factors of access and engagement will help improve the implementation and efficacy of cognitive remediation, and thus the recovery of people with schizophrenia.
Pseudomonas cultivated from Andropogon gerardii rhizosphere show functional potential for promoting plant host growth and drought resilience
Authors: Sarkar Soumyadev , Kamke Abigail , Ward Kaitlyn , Hartung Eli , Ran Qinghong , Feehan Brandi , Galliart Matthew , Jumpponen Ari , Johnson Loretta , Lee Sonny T.M. 
Published: November 2022

Abstract
Background
Climate change will result in more frequent droughts that can impact soil-inhabiting microbiomes (rhizobiomes) in the agriculturally vital North American perennial grasslands. Rhizobiomes have contributed to enhancing drought resilience and stress resistance properties in plant hosts. In the predicted events of more future droughts, how the changing rhizobiome under environmental stress can impact the plant host resilience needs to be deciphered. There is also an urgent need to identify and recover candidate microorganisms along with their functions, involved in enhancing plant resilience, enabling the successful development of synthetic communities.

Results
In this study, we used the combination of cultivation and high-resolution genomic sequencing of bacterial communities recovered from the rhizosphere of a tallgrass prairie foundation grass, Andropogon gerardii. We cultivated the plant host-associated microbes under artificial drought-induced conditions and identified the microbe(s) that might play a significant role in the rhizobiome of Andropogon gerardii under drought conditions. Phylogenetic analysis of the non-redundant metagenome-assembled genomes (MAGs) identified a bacterial genome of interest – MAG-Pseudomonas. Further metabolic pathway and pangenome analyses recovered genes and pathways related to stress responses including ACC deaminase; nitrogen transformation including assimilatory nitrate reductase in MAG-Pseudomonas, which might be associated with enhanced drought tolerance and growth for Andropogon gerardii.

Conclusions
Our data indicated that the metagenome-assembled MAG-Pseudomonas has the functional potential to contribute to the plant host’s growth during stressful conditions. Our study also suggested the nitrogen transformation potential of MAG-Pseudomonas that could impact Andropogon gerardii growth in a positive way. The cultivation of MAG-Pseudomonas sets the foundation to construct a successful synthetic community for Andropogon gerardii. To conclude, stress resilience mediated through genes ACC deaminase, nitrogen transformation potential through assimilatory nitrate reductase in MAG-Pseudomonas could place this microorganism as an important candidate of the rhizobiome aiding the plant host resilience under environmental stress. This study, therefore, provided insights into the MAG-Pseudomonas and its potential to optimize plant productivity under ever-changing climatic patterns, especially in frequent drought conditions.
Combined transcriptome and metabolome analysis revealed pathways involved in improved salt tolerance of Gossypium hirsutum L. seedlings in response to exogenous melatonin application
Authors: Ren Wei , Chen Li , Xie Zong ming , Peng Xiaofeng 
Published: November 2022

Abstract
Background
Salinization is major abiotic stress limiting cotton production. Melatonin (MT) has been implicated in salt stress tolerance in multiple crops including upland cotton. Here, we explored the transcriptomic and metabolomic response of a salt-tolerant self-bred high-yielding cotton line SDS-01, which was exogenously sprayed with four MT concentrations (50, 100, 200, and 500 μM).

Results
Here we found that MT improves plant biomass and growth under salt stress. The combined transcriptome sequencing and metabolome profiling approach revealed that photosynthetic efficiency is improved by increasing the expressions of chlorophyll metabolism and antenna proteins in MT-treated seedlings. Additionally, linoleic acid and flavonoid biosynthesis were improved after MT treatment. The Na+/K+ homeostasis-related genes were increasingly expressed in salt-stressed seedlings treated with MT as compared to the ones experiencing only salt stress. Melatonin treatment activated a cascade of plant-hormone signal transduction and reactive oxygen scavenging genes to alleviate the detrimental effects of salt stress. The global metabolome profile revealed an increased accumulation of flavonoids, organic acids, amino acids and derivatives, saccharides, and phenolic acids in MT-treated seedlings. Interestingly, N, N′-Diferuloylputrescine a known antioxidative compound was highly accumulated after MT treatment.

Conclusion
Collectively, our study concludes that MT is a salt stress regulator in upland cotton and alleviates salt-stress effects by modulating the expressions of photosynthesis (and related pathways), flavonoid, ROS scavenging, hormone signaling, linoleic acid metabolism, and ion homeostasis-related genes.
Assessing clinical trial failure risk factors and reasons in gastric cancer
Authors: Zhang Zikai , Yin Junyi , Yue Yang , Su Yang , Jiang Hong 
Published: November 2022

Abstract
Background
Gastric cancer is one of the leading cancer-related death causes. Enormous efforts have been focused on this field in these years. However, clinical trial failure is becoming a massive obstacle for researchers to apply their research results for clinical use. This study aimed to analyze the reasons behind clinical failures and identify potential risk factors of clinical trial failures.

Methods
On December, 1, 2021, we queried ClinicalTrials.gov for gastric cancer listed in phase II/III. We included trials specifying their interests in “stomach cancer”, “Stomach Neoplasms”, “Gastric Cancer”, “Gastric Neoplasms”, “Gastric Carcinoma”, “Stomach Carcinoma”, “Gastroesophageal Junction Cancer”. Exclude criteria are: (1) Trials that start prior to 01/01/2007 and start after 12/01/2020; (2) Trials with “not yet recruiting”, “suspended”, “withdrawn”, or “unknown” status; (3) Trials do not provide an anticipated accrual number or a start date.

Results
A total of 567 trials are included. 10.2% of these trials are failed. 16 (2.82%) were terminated for good reasons, and 42 (7.41%) were terminated for bad reasons. Multi-centre (P-value = 0.088) and anticipated accrual (P-value = 0.099) are potential risk factors for clinical failures in the simple logistic regression model. After considering the interaction between multi-centre and anticipated accrual, the odds ratio of anticipated accrual is 0.60 (P-value = 0.009) in single centre trials. In multi-centre trials, the odds ratio of anticipated accrual is 0.72 (P-value = 0.025). The primary reason for gastric cancer trial terminations is recruitment failure.

Conclusion
The rate that trials terminated in gastric cancer has decreased compared to previous studies. Comparing to other types of oncology trials, poor accrual continues to be the predominant reason, followed by business or sponsor reasons. Single-center trials with smaller anticipated accrual number are more likely to be terminated which may resulted by limited resources invested to the trial. Single-center design exacerbated the difficulty of participant recruitment. Future studies need to continue tracking the rate of trial termination across oncology and whether the reasons behind them have changed.