bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential volatiles emitted from jasmine plants infested by two spotted spider mite and its attraction to predatory Scirtothrips dorsalis
Authors: Davidson Merlin K. 
Published: August 2022

Abstract
Induction of plant defense in response to herbivore involves emission of synamones that attracts natural enemies of herbivores. Herbivore-Induced volatiles were extracted from fresh healthy jasmine plants (Jasminum sambac L) and infested jasmine plants damaged by two spotted spider mite, Tetranychus urticae Koch. and analysed through Gas Chromatography-Mass spectrometry (GC-MS) to determine volatile hydrocarbon profile in them. The results revealed that both the healthy and damaged leaves had emitted hydrocarbon compounds numbering 19 and 31respectively and some compounds were detected twice or even thrice. The variation in the hydrocarbon constituents of healthy and damaged plants clearly depicts the emission of volatiles responsible for attracting beneficials witnessing tritrophic interactions in jasmine ecosystem. The healthy jasmine plants emanated natural enemy attractants, linalool, methyl salicylate and alpha-farnesene, apart from other volatile organic compounds (VOC). Linalool is a volatile chemical that is produced by herbivore damage that attracts natural enemies. The compound allyl isothiocyanate was detected in two spotted mite infested jasmine extracts at 4.307 mts in a peak area of 1664139 mm2. Allyl isothiocyanate serves the plant as a defense against herbivores; since it is harmful to the plant. Allyl iso thiocyanate detected in enormous quantity in mite infested jasmine plants is a positive cue for commercial preparation of artificial lures to attract mite natural enemies in jasmine ecosystem. In the olfactometer behavioural bioassay studies, maximum number of released mite predator, Scirtothrips sexmaculaus, oriented on mite infested jasmine plant volatiles (18.5 nos), followed by leaf web worm (13.0) and healthy plant volatiles (5.5 nos).
TripletMultiDTI: Multimodal Representation Learning in Drug-Target Interaction Prediction
Authors: Dehghan Alireza , Razzaghi Parvin , Abbasi Karim , Gharaghani Sajjad 
Published: August 2022

Abstract
BackgroundIn drug discovery, drug-target interaction (DTI) plays a crucial role. Identifying DTI in a wet-lab experiment is time-consuming, labor-intensive, and costly. Using reliable computational methods to predict DTI mitigates the enormous costs and time of drug discovery. Deep learning-based methods for predicting DTI have recently gained more attention. ResultsIn this paper, a new multimodal approach to DTI is proposed. It is shown that a discriminative feature representation of the drug-target pair plays the main role in multimodal DTI prediction. To achieve this goal, we propose a new multimodal approach that utilizes triplet loss jointly with the prediction loss. The proposed approach is abbreviately called TripletMultiDTI. The proposed approach has two main contributions: a new architecture that fuses the multimodal knowledge to predict interaction affinity labels and a new loss function that utilizes the triplet loss. Triplet loss encourages clustering of feature space such that similar drug-target pairs have the same feature space and dissimilar drug-target pairs have different feature space. ConclusionsAs a result of our experiments, we were able to improve prediction performance. To this end, the proposed approach is evaluated on a well-known dataset and compared with state-of-the-art multimodal approaches. According to the obtained results, we can perform better than comparable approaches.
Development and validation of a prognostic model for mitophagy-related genes in colon adenocarcinoma: a study based on TCGA and GEO databases
Authors: Tang Yong , Guo Haiyang , Chen Lin , Wang Xiaobo , Chen Qi , Gou Lei , Liu Xiuyu , Wang Xianfei 
Published: August 2022

Abstract
Background:Mitophagy is the degradation of dysfunctional mitochondria. Its dysregulation can lead to an accumulation of damaged mitochondria and is implicated in carcinogenesis and tumorigenesis. Despite increasing evidence that mitophagy plays a role in colon tumorigenesis, the role of mitophagy-related genes (MRGs) in colon adenocarcinoma (COAD) prognosis and treatment remains largely unknown. Methods: As a first step, we extracted 1899 mitophagy-related genes from GeneCards and screened 461 differentially expressed genes from TCGA database. Using Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene Ontology terms, we constructed a protein-protein interaction network, and conducted key module screening for the differential genes. Cox regression and least absolute shrinkage and selection operator analyses were used to identify PPARGC1A, SLC6A1, EPHB2 and PPP1R17 as prognosis-related genes. We assessed the feasibility of prognostic models using Kaplan-Meier analysis, time-dependent receiver operating characteristics, risk scores, Cox regression analysis, and principal component analysis. We tested the model using the GEO database and constructed nomograms for future clinical applications. At 1, 3, and 5 years, the area under the receiver operating characteristic curve for the TCGA cohort was 0.628, 0.678, and 0.755, whereas that of the GEO cohort was 0.609, 0.634, and 0.640. In addition, we compared immune cell infiltration between the two groups and assessed sensitivity to treatment with many commonly used chemotherapeutic agents in individuals with different risk factors. Finally, we performed qualitative reverse transcription polymerase chain reaction to examine the expression of prognosis-related MRGs. Results: In this study, we investigated the potential value of MRGs in patients with colon adenocarcinoma at the gene level. Utilizing The Cancer Genome Atlas (TCGA), we developed a mitophagy-related gene model to predict the prognosis of COAD, and examined this risk model using the Gene Expression Omnibus (GEO). Conclusions: This study has successfully constructed a mitophagy-related gene signature with significant predictive value for COAD and provides new possibilities for its future treatment.
Responses to heat waves: what can Twitter data tell us?
Authors: Zander Kerstin Katharina , Rieskamp Jonas , Mirbabaie Milad , Alazab Mamoun , Nguyen Duy 
Published: August 2022

Abstract
Increasing average temperatures and heat waves have devasting impacts on human health and well-being but studies on heat impacts and how people adapt are rare and often confined to specific locations. In this study we aimed to explore how social media data can be used to generate knowledge about how people feel about heat waves through an analysis of their conversations. We collected global Twitter data over two months (January and February 2022) using predefined hashtags about heatwaves. Topic modelling revealed five clusters of which three were related to communications about climate change related heat, covering 76% of all tweets. These three clusters included one on the impacts of heat and heatwaves (34%), one on perceptions of weather and heat (28%) and one on extreme weather and climate change (14%). The remaining 24% of tweets were on sport events or films and music. The number of tweets in each week corresponded well with major heat wave occurrences in Argentina, Australia and India, indicating that people are aware of the threat from heat and its impacts on the society, although not much could be learned in terms of personal coping and preparedness strategies. Apart from the content of tweets, a great deal could be learned in terms of how people engaged with Twitter tweets about heat or heat waves. We found that tweets posted early, and which were shared by other influential Twitter users, were among the most popular. Finally, we found that the most popular tweets belonged to individual scientists or respected news outlets, with no evidence of wide-spread misinformation about climate change related heat.
The kinetics and fragmentation of cell-free DNA from cultured cancer cells are influenced by anticancer treatments
Authors: Bustamante Prisca , Abdouh Mohamed , Tsering Thupten , Burnier Julia V 
Published: August 2022

Abstract
Liquid biopsy-based detection of circulating free DNA (cfDNA) is a promising tool to monitor tumor progression and treatment response. cfDNA release is thought to result from a combination of cell death (apoptosis and necrosis), and active cellular secretion. As such, cytotoxic anti-cancer therapies can impact cfDNA kinetics. This makes the interpretation of cfDNA analyses pivotal for its applicability as a biomarker. In this study, we assessed the kinetics and fragmentation of cfDNA in cancer cells of various origins following standard anti-cancer treatments with different cytotoxic effects and mechanisms of action.Human colorectal carcinoma, lung adenocarcinoma, and uveal melanoma cancer cells were subjected to different forms of cytotoxic stress, including induction of apoptosis (tumor necrosis factor (TNF)-related apoptosis-inducing ligand (Apo2L/TRAIL)), cell cycle arrest (Roscovitine, Valproic acid), necrosis (radiotherapy), and senescence. Following treatments, cells were analyzed for their cell cycle progression, level of senescence, and mechanism of cell death (apoptosis vs. necrosis). Heat treatment was used as a control for necrosis-related cell death. Total cfDNA and mutant cfDNA (based on the mutations of each parental cell line) were isolated from cultured media and quantified using the Qubit assay and digital droplet PCR targeting, respectively. Fragment length of the isolated cfDNA was visualized using the Bioanalyzer 2100.Total and mutant cfDNA levels increased during all cytotoxic treatments in a concentration-dependent manner. Notably, cells undergoing apoptosis shed higher levels of cfDNA compared to necrotic and senescent cells. In addition, electropherogram images showed that cytotoxic conditions alter fragment size distribution, with smaller fragments of <200bp associated with apoptosis and >1000 bp with necrosis.The kinetics and fragmentation of released cfDNA are influenced by cytotoxic insults. Determining the characteristics of cfDNA can facilitate and improve its use as a clinical biomarker during anti-cancer therapy. Our data pave the way for the establishment of criteria to apply when monitoring cfDNA for cancer management based on the anticancer therapeutic strategy.
Nurses’ experiences with closeness, competence and patient focus in digital care meetings: A qualitative study
Authors: Carlsson Malin , Kjällman-Alm Annika , Holmström Malin Rising 
Published: August 2022

Abstract
Background: Health care’s rapid transition from in-person visits to digital care meetings has challenged nurses to find new, sustainable ways of using digital technology.Methods: The aim was to describe nurses’ experiences with person-centred care and competence in digital care meetings over time. In this study, a qualitative longitudinal descriptive explorative design was used, and 16 individual interviews were carried out with nurses. Data were analysed using qualitative content analysis. The COREQ checklist was used in this study.Results: The results were presented in four themes: being open to change and new ways of working with patients; struggling to handle requirements; developing new ways of working and focusing on patients despite the distance.Conclusions: The Digital Competence in Care course helped develop nurses’ skills and the practice of digital care meetings. Training in digital care theory increased nurses’ competence and facilitated the creation of new knowledge. The nurses’ professional role was strengthened by having increased power in decision-making and the ability to participate in changing work routines. Digital care meetings were shown to be distance bridging and complementary to physical care meetings contributing to person-centred care. The increased availability of health care via digital means has affected the consumption of care and tailored education needs for nurses must be met by nursing education programs. Digital care is accessible, efficient and enables care regardless of geographical conditions, its innovative development needs to be based on science and experience and nurses are key personnel in this process. Trial registration: Not applicable.
Transcriptome analysis reveals dysregulation of immune and inflammatory responses in DRG and spinal cord of a mouse model of acute herpetic neuralgia
Authors: Wu Songbin , Yang Shaomin , Li Rongzhen , Ba Xiyuan , Jiang Changyu , Xiong Donglin , Xiao Lizu , Sun Wuping 
Published: August 2022

Abstract
Objective.
Herpetic-related neuralgia (HN) caused by varicella-zoster virus (VZV) infection is one of the most typical and common neuropathic pain in the clinic. However, due to the lack of an animal model to simulate HN, the potential mechanisms and therapeutic approaches for the prevention and treatment of HN are still unclear. This study aims to provide a comprehensive understanding of the molecular mechanisms and potential therapeutic targets of HN.
Methods.
We used an HSV-1 infection-induced HN mouse model and screened the differentially expressed genes (DEGs) in the DRG and spinal cord using an RNAseq technique. Moreover, the bioinformatics methods were used to figure out the signaling pathways and expression regulation patterns of the DEGs enriched. In addition, quantitative real-time RT-PCR and western blot was carried out to further confirm the expression of DEGs.
Results.
HSV-1 inoculation in mice resulted in mechanical allodynia, thermal hyperalgesia, and cold allodynia, following the infection of HSV-1 in both DRG and spinal cord. Besides, HSV-1 inoculation induced an up-regulation of ATF3, CGRP, and GAL in DRG and activation of astrocytes and microglia in the spinal cord. Moreover, 639 genes were up-regulated, 249 genes were down-regulated in DRG, whereas 534 genes were up-regulated, and 12 genes were down-regulated in the spinal cord of mice 7 days after HSV-1 inoculation. GO and KEGG enrichment analysis suggested that immune responses and cytokine-cytokine receptor interaction are involved in DRG and spinal cord neurons in mice after HSV-1 infection. In addition, CCL5 and its receptor CCR5 were significantly up-regulated in DRG and spinal cord upon HSV-1 infection in mice. And blockade of CCR5 exhibited a significant analgesic effect and suppressed the up-regulation of inflammatory cytokines in DRG and spinal cord induced by HSV-1 infection in mice.
Conclusions.
We reported a reliable HN model with HSV-1 infection-induced allodynia and hyperalgesia in mice through dysregulation of immune response and cytokine-cytokine receptor interaction mechanism.
Risk Factors for E2SKAPE Infections and Mortality among Liver Transplant Recipients
Authors: Wu Xiaoxia , Peng Weiting , Long Guo , Wu Lingli , Lin Shu , Wan Qiquan 
Published: August 2022

Abstract
Introduction: Infections caused by Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp (ESKAPE) plus Escherichia coli (E2SKAPE), in particular multidrug-resistant (MDR) E2SKAPE infections, occur frequently and pose a life-threatening to liver transplant (LT) recipients. To prevent E2SKAPE infections and improve the prognosis of LT recipients, the identification of risk factors for E2SKAPE infections and mortality is necessary.Methods: E2SKAPE pathogens were isolated and identified from clinical samples following standard microbiological procedures. All episodes of E2SKAPE infections and mortality documented among LT recipients were analyzed. Results: A total of 83 episodes of E2SKAPE infections, including 75 (90.4%) episodes of MDR-E2SKAPE infections, occurred in 23.1% (53/229) of LT recipients. E. faecium was the dominant causative bacterium (37/83; 44.6%). The most common site of infections was the urinary tract (14/53; 26.4%). Sixteen (7%) patients died within 2 months after LT, and 7 deaths were E2SKAPE infections-related. Multivariate logistic regression analysis revealed that female sex [odds ratio (OR) = 3.665, 95% confidence interval (CI): 1.614-8.321, P = 0.002], duration of surgery ≥ 400 min [OR = 2.328, 95%CI: 1.151-4.707, P = 0.019], intraoperative red blood cell (RBC) transfusion ≥ 12U [OR = 2.542, 95%CI: 1.218-5.306, P = 0.013] and indwelling urethral catheter use ≥ 3 days [OR = 3.96, 95%CI: 1.309-11.981, P = 0.015] were independent risk factors for E2SKAPE infections after LT, and that only exposure to more than two intravenous antibiotics post-LT [OR =0.318, 95%CI: 0.15-0.674, P = 0.003] was negatively associated with acquisition of E2SKAPE infections. The predictors of crude mortality included female sex [OR = 4.822, 95%CI: 1.299-17.904, P = 0.019], creatinine on day 3 post-LT > 1.5 mg/dL [OR = 11.014, 95%CI: 2.985-40.637, P < 0.001], mechanical ventilation post-LT [OR = 10.724, 95%CI: 2.695-42.676, P = 0.001] and recipients with E2SKAPE infections [OR = 4.112, 95%CI: 1.169-14.47, P = 0.028].Conclusion: A high incidence of E2SKAPE infections was noted in the early post-LT period. The most common infection site was the urinary tract, and the dominant pathogenic bacterium was E. faecium. Female sex, prolonged surgery time, massive RBC transfusion, or delayed urethral catheter removal were associated with E2SKAPE infections. Only exposure to more than two intravenous antibiotics post-LT was negatively related to the acquisition of E2SKAPE infections. The predictors of mortality included female sex, creatinine on day 3 post-LT > 1.5 mg/dL, mechanical ventilation post-LT, and recipients with E2SKAPE infections.
Antifungal Activity of Apiaceae Family plants against Aspergillus fumigates and Fusorium solani
Authors: tripathi sakshi 
Published: August 2022

Abstract
The study’s objective was to determine whether ten plants from the Apiaceae family used in traditional medicine had any antifungal properties against the fungi A. fumigates and F. solani. The selection of the plants was based on their alleged ethnobotanical applications. The antifungal activity of several plant extracts in methanol, ethanol, hexane and ethyl acetate against A. fumigates and F. solani was tested. We employed the Agar well diffusion method to assess the antifungal effectiveness. Extracts’ Minimum inhibitory concentrations (MIC) were established. Plant extracts in methanol and ethanol were more efficient than those in hexane and ethylacetate The findings indicated that Coriander sativum L., Pimpinella anisum L., Bunium persicum L. and Cuminum cyminum L had the strongest antifungal effects. Comparatively little activity was seen for the extract of Carum carvi L. and Centella asiatica L. against A. fumigates and F. solani. Extracts’ MIC values were found to range from 0.36 µg/ml to 0.14 µg/ml. Extracts with the largest zone of inhibition may be a source of antifungal compounds that can be used to treat human infection. We come to the conclusion that the remarkable fungicidal characteristics of these extracts support their traditional usage as an antiseptic, medicinal and preservative.
A common IGF1R gene variant predicts later life breast cancer risk in women with preeclampsia
Authors: Powell Mark , Fuller Sophia , Gunderson Erica , Benz Christopher 
Published: August 2022

Abstract
PurposePreeclampsia has been inconsistently associated with altered later-life risk of cancer. This study utilizes the Nurses’ Health Study 2 to determine if the future risk of breast and non-breast cancers in women who experience preeclampsia is modified by carrying a protective variant of rs2016347, a functional insulin-like growth factor receptor-1 (IGF1R) single nucleotide polymorphism. MethodsThis retrospective cohort study enrolled participants in 1989 and followed them through 2015, with a study population of 86,751 after exclusions. Cox proportional hazards models both with and without the impact of rs2016347 genotype were used to assess the risk of all breast cancer, hormone receptor-positive (HR+) breast cancer, and non-breast cancers. ResultsWomen with preeclampsia had no change in risk of all breast, HR+ breast, or non-breast cancers when not considering genotype. However, women carrying at least one T allele of rs2016347 had a lower risk of HR+ breast cancer, HR = 0.67, 95% CI: 0.47-0.97, P = 0.04, with interaction term P = 0.06. For non-breast cancers as a group, women carrying a T allele had an HR = 0.76, 95% CI: 0.53-1.08, P = 0.12, with interaction term P = 0.26. ConclusionsThis retrospective cohort study found that women with preeclampsia who carry a T allele of IGF1R rs2016347 had a reduced future risk of developing HR+ breast cancer, and a reduced but not statistically significant decreased risk of non-breast cancers suggesting a possible role for the IGF-1 axis in the development of cancer in these women.
Validity and reliability of the physiological and perceptual responses elicited during a novel treadmill-based Soccer Referee Simulation (SRS)
Authors: McEwan Gary P. , Unnithan Viswanath B. , Easton Chris , Glover Andrew J. , Arthur Rosie 
Published: August 2022

Abstract
Purpose: This study explored the validity and reliability of the physiological and perceptual responses elicited during a novel treadmill-based Soccer Referee Simulation (SRS).Methods: Following the collection of baseline measures and habituation procedures, eight sub-elite soccer referees completed a single trial of the SRS whereby measures of heart rate (HR), oxygen uptake (V̇O2), blood lactate concentrations ([La-]b), and differential ratings of perceived exertion (RPE) were obtained. Referees’ HR responses were also monitored during a series of competitive matches (5 match observations per referee). For the reliability aspect of the investigation, eight well-trained males with soccer playing experience and a comparable physiological profile were initially habituated, and thereafter completed three separate trials of the SRS during which the reliability of the selected outcome variables were ascertained. Trials were separated by 3-7 days and performed under standardised conditions. Results: No differences were evidenced between the SRS and match play in relation to measures of mean HR (P=0.444; ES=0.29), peak HR (P=0.074; ES=0.74), or HR-based training impulse (P=0.498; ES=0.25). Additionally, no systematic differences were detected between reliability trials for any of the measured outcome variables (P≥0.293), whilst good levels of reliability were observed for measures of mean HR (ICC=0.94; CV=3.1%), peak HR (ICC=0.93; CV=2.2%), HR-based training impulse (ICC=0.95; CV=10.0%), mean V̇O2 (ICC=0.95; CV=2.6%); [La-]b (ICC≥0.89; CV≤11.5%), and differential RPE (ICC≥0.94; CV≤15.1%). Conclusion: The SRS represents a valid and reliable protocol that closely replicates the physiological and decision-making demands of soccer refereeing.