bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desigualdade e criminalização da pobreza: questões sobre educação e desequilíbrio social Entrevista com Fernando Codoceo
Authors: Silva Bruna Ercoles da , Pátaro Ricardo Fernandes 
Published: June 2022

Fernando Codoceo Ortiz é licenciado em Filosofia pela Universidade de Valparaíso, no Chile, e doutor em Ciências Políticas pela Universidade de Aachen, na Alemanha. Atualmente, é professor da Universidade de Los Lagos, em Osorno, Chile. A Universidade de Los Lagos é uma instituição estatal de ensino superior, criada em 30 de agosto de 1993, com sede em Osorno e campus em Santiago, Puerto Montt e Chiloé. Oferece cursos de graduação nas áreas de Ciências Exatas, Educação e Pedagogia, Ciências Sociais, Arquitetura, Ciências Biológicas e Biodiversidade, Ciências da Atividade Física, Humanidades e Artes, Governo e Empresa, Ciências Administrativas e Econômicas.
O tratamento das especificidades da modalidade oral da língua portuguesa no Ensino Fundamental II
Authors: Botler Laís Maria Álvares Rosal , Suassuna Lívia 
Published: June 2022

No presente artigo, objetivamos, com base em Marcuschi (2003, 2010) e Dolz, Schneuwly e Haller (2004), entre outros autores, descrever e analisar como duas professoras do segundo segmento do ensino fundamental abordam a modalidade oral da língua em suas aulas. Desenvolvemos uma pesquisa qualitativa, com foco na maneira como as docentes tratavam as especificidades da oralidade. A análise dos dados mostrou que ambas se preocupavam em mostrar para os alunos que há formas diferentes de utilização da língua, a depender da modalidade. Além disso, suas práticas contribuíram para uma caracterização mais sólida e bem justificada da modalidade oral da língua.
Simultaneous Detection of Pathogens and Tumors in Patients With Suspected Infections by Next-Generation Sequencing
Authors: Su Jiachun , Han Xu , Xu Xiaogang , Ding Wenchao , Li Ming , Wang Weiqin , Tian Mi , Chen Xiyuan , Xu Binbin , Chen Zhongqing , Yuan Jinyi , Qin Xiaohua , Lin Dongfang , Wang Ruilan , Gong Ye , Pan Liping , Wang Jun , Wang Minggui 
Published: June 2022

BackgroundDifferential diagnosis of patients with suspected infections is particularly difficult, but necessary for prompt diagnosis and rational use of antibiotics. A substantial proportion of these patients have non-infectious diseases that include malignant tumors. This study aimed to explore the clinical value of metagenomic next-generation sequencing (mNGS) for tumor detection in patients with suspected infections.MethodsA multicenter, prospective case study involving patients diagnosed with suspected infections was conducted in four hospitals in Shanghai, China between July 2019 and January 2020. Based upon mNGS technologies and chromosomal copy number variation (CNV) analysis on abundant human genome, a new procedure named Onco-mNGS was established to simultaneously detect pathogens and malignant tumors in all of the collected samples from patients.ResultsOf 140 patients screened by Onco-mNGS testing, 115 patients were diagnosed with infections; 17 had obvious abnormal CNV signals indicating malignant tumors that were confirmed clinically. The positive percent agreement and negative percent agreement of mNGS testing compared to clinical diagnosis was 53.0% (61/115) and 60% (15/25), vs. 20.9% (24/115) and 96.0% (24/25), respectively, for conventional microbiological testing (both P &lt;0.01). Klebsiella pneumoniae (14.8%, 9/61) was the most common pathogen detected by mNGS, followed by Escherichia coli (11.5%, 7/61) and viruses (11.5%, 7/61). The chromosomal abnormalities of the 17 cases included genome-wide variations and local variations of a certain chromosome. Five of 17 patients had a final confirmed with malignant tumors, including three lung adenocarcinomas and two hematological tumors; one patient was highly suspected to have lymphoma; and 11 patients had a prior history of malignant tumor.ConclusionThis preliminary study demonstrates the feasibility and clinical value of using Onco-mNGS to simultaneously search for potential pathogens and malignant tumors in patients with suspected infections.
Sex-Specific Associations of Anxiety With Left Ventricular Hypertrophy and Transmural Dispersion of Repolarization in Hypertensive Patients
Authors: Zhu Ling , Cui Qianwei , Zhang Yong , Liu Fuqiang , Zhao Jingsha , Wang Junkui 
Published: June 2022

BackgroundThe previous studies have shown that individuals with hypertension and anxiety have a higher mean left ventricular mass index (LVMI) and QTc dispersion. We explored the associations between anxiety and left ventricular hypertrophy (LVH) and between anxiety and transmural dispersion of repolarization (TDR) (as detected by T peak-T end interval/QT interval, Tp–Te/QT ratio) in patients with hypertension.MethodsA total of 353 patients with uncomplicated hypertension from the Shaanxi Provincial People’s Hospital were enrolled between 2017 and 2021. Anxiety was defined as a Hamilton Anxiety Scale (HAM-A) score ≥ 14. Logistic regression models were used to estimate the association between HAM-A and LVH. The association between HAM-A score and Tp–Te/QT was estimated using linear regression.ResultsParticipants were divided into two groups based on the presence of anxiety. LVMI was significantly higher in patients with hypertension and anxiety than in those with hypertension without anxiety (no anxiety: 84.36 ± 23.82, anxiety: 105.75 ± 25.45 g/m2, p &lt; 0.001). HAM-A score was positively correlated with LVMI (r = 0.578, p &lt; 0.001) and with Tp–Te/QT (r = 0.252, p &lt; 0.001). Logistic regression models showed that patients with hypertension and anxiety were at higher risk of LVH than were patients with hypertension without anxiety (adjusted OR, 2.44, 95% CI, 1.35–4.43, p = 0.003). The linear regression analysis showed that the HAM-A score was associated with Tp–Te/QT ratio (adjusted β, 0.001, 95% CI, 0.001–0.002, p = 0.013). There was an interaction between sex and anxiety for LVH risk (p for interaction = 0.035) and for increased Tp–Te/QT (p for interaction = 0.014). After stratification by sex, anxiety was associated with increased risk for LVH in men with hypertension (adjusted OR, 5.56, 95% CI, 2.07–14.98, p = 0.001), but not in women (adjusted: OR, 1.44, 95% CI, 0.64–3.26, p = 0.377) with hypertension. The HAM-A score was also positively associated with Tp–Te/QT ratio in male (adjusted β, 0.002, 95% CI, 0.001–0.003, p &lt; 0.001), but not in women (adjusted β, 0.001, 95% CI, –0.0002–0.002, p = 0.165).ConclusionOur results indicated that anxiety was associated with LVH and with increased TDR in men with hypertension, but not in women with hypertension.
An Electrocardiographic Characterization of Left Bundle Branch Area Pacing-Induced Right Ventricular Activation Delay: A Comparison With Native Right Bundle Branch Block
Authors: Ozpak Emine , Demolder Anthony , Kizilkilic Sevda , Calle Simon , Timmermans Frank , De Pooter Jan 
Published: June 2022

BackgroundLeft bundle branch area pacing (LBBAP) induces delayed RV activation and is thought to be harmless, since the electrocardiographic signature is reminiscent to native RBBB. However, to what extent the delayed RV activation during LBBAP truly resembles that of native RBBB remains unexplored.MethodsThis study included patients with incomplete RBBB (iRBBB), complete RBBB (cRBBB) and patients who underwent LBBAP. Global and right ventricular activation times were estimated by QRS duration and R wave peak time in lead V1 (V1RWPT) respectively. Delayed RV activation was further characterized by duration, amplitude and area of the terminal R wave in V1.ResultsIn patients with LBBAP (n = 86), QRS duration [120 ms (116, 132)] was longer compared to iRBBB patients (n = 422): 104 ms (98, 110), p &lt; 0.001, but shorter compared to cRBBB (n = 223): 138 ms (130, 152), p &lt; 0.001. V1RWPT during LBBAP [84 ms (72, 92)] was longer compared to iRBBB [74 ms (68, 80), p &lt; 0.001], but shorter than cRBBB [96 ms (86, 108), p &lt; 0.001]. LBBAP resulted in V1 R′ durations [42 ms (28, 55)] comparable to iRBBB [42 ms (35, 49), p = 0.49] but shorter than in cRBBB [81 ms (68, 91), p &lt; 0.001]. During LBBAP, the amplitude and area of the V1 R′ wave were more comparable with iRBBB than cRBBB. V1RWPT during LBBAP was determined by baseline conduction disease, but not by LBBAP capture type.ConclusionLBBAP-induced delayed RV activation electrocardiographically most closely mirrors the delayed RV activation as seen with incomplete rather than complete RBBB.
An Atypical Anomalous Aortic Origin of the Left Coronary Artery With Intra-Arterial Wall Course Pretending a Normal Migration on Imaging Screening: A Case Report
Authors: Hu Fan , Wang Xinyue , Wan Jiaojiao , Li Yifei , Wang Tao , Zhou Kaiyu , Shi Xiaoqing , Liu Zhongqiang , Fang Jie , Hua Yimin 
Published: June 2022

BackgroundAn anomalous aortic origin of a coronary artery (AAOCA) has been considered as a dominant cause of sudden cardiac death (SCD) among young age children. Therefore, it is critical to identify AAOCA timely to avoid lethal events. Recently, accumulating cases of right or left coronary arteries originating from inappropriate locations at the sinus of Valsalva have been identified. Here, we report a rare case of AAOCA with an intra-arterial wall course pretending normal migration on imaging screening in a patient who suffered from syncope.Case summaryA 7-year-old male without a previous history of cardiovascular and cerebrovascular diseases suddenly suffered from sharp chest pain and syncope after intensive exercise. The electrocardiogram showed that the ST segment of multiple leads was depressed by more than 0.05 mV, and biomarkers indicated severe myocardial injuries. The left ventricular ejection fraction (LVEF) decreased dramatically to 23%. Fulminant myocarditis and cardiomyopathy were therefore excluded. However, a relatively normal coronary artery origin, which arose from the left coronary sinus, presented on echocardiography and cardiac CT angiography (CTA). It is difficult to draw an association between severe clinical manifestations and slight malformations on echocardiography and CTA. Furthermore, selective coronary angiography revealed that an anomalous left coronary artery arose from the superior margin of the inappropriate sinus, developed an intramural wall course and finally exits the left sinus of Valsalva and migrated between the aorta and the pulmonary artery, which induced severe myocardial infarction during exercise. Then, the patient received surgical correction with a modified unroofing procedure. After 2 months of intensive treatment, the patient was discharged and remained asymptomatic through 18 months of follow-up.ConclusionAAOCA, especially anomalous left coronary artery (ALCA), represents a major potential risk of SCD. We reported an atypical manifestation of ALCA arising from the inappropriate sinus of Valsalva and merging into the intra-arterial wall to develop a strange course and then sprout between the aorta and the pulmonary artery. The diversity of AAOCA might present as a relatively normal course under non-invasive radiological imaging scanning.
Relationship Between Nb-Ta Enrichments and Highly-Fractionated Granitic Magma Evolution in South China: Geochronological, Zircon Hf Isotopic and Geochemical Evidence From Jianfengling Granite
Authors: Ze-Yi Chen , Yong-Jun Shao , Han-Tao Wei , Cheng Wang 
Published: June 2022

The Jianfengling granite is an important ore-forming rock unit in the Xianghualing orefield in Nanling region (South China), and its magmatic evolution is vital to understand the regional niobium (Nb)-tantalum (Ta) mineralization. The Jianfengling granite comprises three gradual transitional lithofacies: porphyritic biotite monzogranite (mesophase facies), biotite monzogranite (transition facies) and topaz biotite monzogranite (central facies). All the three biotite monzogranite lithofacies are characterized by being rich in SiO2 and Al2O3, poor in TFe2O3, CaO, MgO, TiO2, MnO and P2O5, and are peraluminous high-K calc-alkaline. These rocks are remarkably enriched in Rb, Th, U, Nb and Ta, strongly depleted in Ba and Sr, and slightly depleted in Zr. The LREE/HREE decreases gradually from porphyritic biotite monzogranite, through biotite monzogranite to topaz biotite monzogranite, with increasing convex (“M-type”) lanthanide tetrad effect, which similar to the highly-fractionated granite. The high Th-U (Thavg. = 2051.56°ppm; Uavg. = 1,498.23°ppm, respectively) and low Th-U (Thavg. = 708.14°ppm; Uavg. = 441.57°ppm, respectively) zircon grains from porphyritic biotite monzogranite yielded weighted average 206Pb/238U ages of 161.3 ± 1.6 Ma (MSWD = 1.3, n = 13) and 158.7 ± 2.0 Ma (MSWD = 1.7, n = 13), respectively, consistent with the large-scale magmatic-mineralization event in the Nanling region (ca. 160–150 Ma). The εHf(t) values of the high Th-U (-6.48 to -2.51) and low Th-U (-6.58 to -1.12) zircon grains from the porphyritic biotite monzogranite indicate that the causative magma was formed from partial melting of the Mesoproterozoic Cathaysian basement rocks in a lithospheric extension setting. The Nb and Ta contents increase with fluorine from the porphyritic to topaz biotite monzogranite, indicating that the Nb-Ta enrichment may have caused by the gradual increase of fluxing content (fluorine) during the fractionation of the Jianfengling granitic magma.
A Novel Somatic Mutation of CACNA1H p.V1937M in Unilateral Primary Hyperaldosteronism
Authors: Tseng Chi-Shin , Peng Kang-Yung , Wang Shuo-Meng , Tsai Yao-Chou , Huang Kuo-How , Lin Wei-Chou , Hu Ya-Hui , Wu Vin-Cent , Chueh Jeff S. 
Published: June 2022

BackgroundSomatic mutations for excess aldosterone production have been frequently identified as important roles in the pathogenesis of unilateral primary hyperaldosteronism (uPA). Although CACNA1H mutation represents a minor etiology in primary aldosteronism, it plays a significant role in causing uPAs in sporadic cases.ObjectiveTo identify novel somatic CACNA1H mutation in patients with uPA and investigate the pathophysiological, immunohistological, and clinical characteristics of the variant.MethodsWe applied a customized and targeted gene panel next-generation sequencing approach to detect mutations from the uPA cohort in Taiwan Primary Aldosteronism Investigation study group. Information from pre-diagnostic to postoperative data was collected, including past history, medications, blood pressure readings, biochemical data, and image studies. The functional role of the variant was confirmed by in vitro studies, demonstrating aldosterone production in variant-transfected human adrenal cell lines.ResultsWe identified a novel somatic CACNA1H mutation c.5809G&gt;A (p.Val1937Met) in a uPA case. The CACNA1H gene encodes the pore-forming alpha-1H subunit of the voltage-dependent T-type calcium channel Cav3.2. This somatic CACNA1H p.V1937M variant showed excellent clinical and biochemical outcomes after ipsilateral adrenalectomy. The functional effect of somatic CACNA1H p.V1937M variant results in increased CYP11B2 expression and aldosterone biosynthesis in HAC15 cells. A distinct heterogeneous foamy pattern of CYP11B2 and CYP17A1 expression was identified in immunohistological staining, supporting the pathological evidence of aldosterone synthesis.ConclusionsThe somatic mutation of CACNA1H p.V1937M might be a pathogenic driver in aldosterone overproduction. This study provides new insight into the molecular mechanism and disease outcomes of uPA.
Neoadjuvant B-RAF and MEK Inhibitor Targeted Therapy for Adult Papillary Craniopharyngiomas: A New Treatment Paradigm
Authors: Calvanese Francesco , Jacquesson Timothée , Manet Romain , Vasiljevic Alexandre , Lasolle Hélène , Ducray Francois , Raverot Gerald , Jouanneau Emmanuel 
Published: June 2022

BackgroundSurgical and clinical management of craniopharyngiomas is associated with high long-term morbidity especially in the case of hypothalamic involvement. Improvements in knowledge of craniopharyngioma molecular biology may offer the possibility of safe and effective medical neoadjuvant treatments in a subset of patients harboring papillary subtype tumors with a BRAFV600E mutation.MethodWe report herein two cases of tubero-infundibular and ventricular Papillary Craniopharyngiomas in which BRAF/MEK inhibitor combined therapy was used as adjuvant (Case 1) or neoadjuvant (Case 2) treatment, with a 90% reduction in tumor volume observed after only 5 months. In Case 2 the only surgical procedure used was a minimal invasive biopsy by the trans-ventricular neuroendoscopic approach. As a consequence, targeted therapy was administered in purely neoadjuvant fashion. After shrinkage of the tumor, both patients underwent fractionated radiotherapy on the small tumor remnant to achieve long-term tumor control. A review of a previously reported case has also been performed.ResultThis approach led to tumor control with minimal long-term morbidity in both cases. No side effects or complications were reported after medical treatment and adjuvant radiotherapy.ConclusionOur experience and a review of the literature argue for a change in the current treatment paradigm for Craniopharyngiomas (CPs). In giant and invasive tumors, confirmation of BRAFV600E mutated PCPs by biopsy and BRAF/MEK inhibitor therapy before proposing other treatments may be useful to improve long term outcomes for patients.
The Positive Relationship Between the Low-Density Lipoprotein Cholesterol/Apoprotein B Ratio and Bone Turnover Markers in Patients With Type 2 Diabetes
Authors: Lu Chun-feng , Liu Wang-shu , Huang Hai-yan , Ge Xiao-qin , Hua Ling-yan , Wang Xue-qin , Su Jian-bin 
Published: June 2022

BackgroundDyslipidemia may contribute to low bone turnover in patients with type 2 diabetes (T2D) through mediating oxidative stress and atherosclerosis. The low-density lipoprotein cholesterol/apoprotein B (LDL-C/Apo B) ratio is a surrogate marker of small and density low-density lipoprotein cholesterol (sd-LDL-C), a most harmful group of LDL-Cs. The present study aimed to investigate the association between the LDL-C/Apo B ratio and bone turnover in patients with T2D.MethodsThis study was a cross-sectional study enrolled patients with T2D from January 2021 to December 2021. Each participant was assessed for lipid profiles, bone turnover markers (BTMs), lumbar spine (L1-L4) and hip dual-energy X-ray absorptiometry (DXA) scans. Osteoporosis was diagnosed as a T-score lower than or equal to -2.5 at the spine or hip.ResultsA total of 335 patients with T2D were enrolled in the study, and the LDL-C/Apo B ratio ranged from 0.78 to 4.00. Along with the LDL-C/Apo B ratio tertile ascending, osteocalcin (OC), C-terminal telopeptide (CTx) and N-terminal propeptide of type-I procollagen (PINP) levels gradually increased (all p &lt; 0.05). There were no differences in lumbar spine and hip T-score, proportion of osteoporosis (all p &gt; 0.05) among the three subgroups. The LDL-C/Apo B ratio was positively correlated with lnOC (r = 0.244, p &lt; 0.001), lnCTx (r = 0.226, p &lt; 0.01) and lnPINP (r = 0.211, p &lt; 0.001). These significant positive correlations persisted even when divided into male and female subgroups. Furthermore, three multiple linear regression analyses were constructed to investigate the independent association of the LDL-C/Apo B ratio with the BTMs levels. After adjusting for other clinical parameters, the LDL-C/Apo B ratio was still significantly associated with OC level (β = 0.199, t = 3.348, p &lt; 0.01), CTx level (β = 0.238, t = 4.084, p &lt; 0.001) and PINP level (β = 0.162, t = 2.741, p &lt; 0.01).ConclusionThe LDL-C/Apo B ratio was significantly and positively associated with BTMs in patients with T2D. In clinical practice, more attention should be paid to the patients with T2D whose LDL-C/Apo B ratio is relatively low for the purpose of maintaining bone health.
m7G-Related DNA Damage Repair Genes are Potential Biomarkers for Predicting Prognosis and Immunotherapy Effectiveness in Colon Cancer Patients
Authors: Chen Shuran , Dong Rui , Li Yan , Zheng Ni , Peng Guisen , Lu Fei , Qiu Quanwei , Wen Hexin , Wang Yitong , Wu Huazhang , Liu Mulin 
Published: June 2022

Objective: m7G is a post-transcriptional modification modality, however, limited research has been conducted on its role in colon cancer. DNA damage repair (DDR) is an important factor that contributes to colon cancer development, growth and chemoresistance. This study aimed to explore whether m7G-related DNA damage repair genes may be used as biomarkers to predict the prognosis of colon cancer patients.Methods: We use non-negative matrix factorization (NMF) to type CRC patients into. Risk models were constructed using different expression genes in two clusters. We assessed the reliability of risk models with DCA curves, and a Nomogram. Meanwhile, The receiver operating characteristic and C-index curves were used to compare the predictive significance of the constructed risk models with other studies. In additional, we examined the significance of risk models on patients’ immunity microenvironment and response to immune therapy. Finally, we used a series of cellular experiments to validate the effect of model genes on the malignant progression of CRC cells.Results: Twenty-eight m7G genes were obtained from the GSEA database. Multivariate Cox and LASSO Cox regression analysis was performed and eleven m7G-related DDR genes were identified for constructing the risk model. Survival and stage of CRC patients were worser in the high-risk group than in the low-risk group for both the training and test sets. Additionally, the different immune microenvironment status of patients in the high- and low-risk groups, suggesting that patients in the low-risk group may be more sensitive to immunotherapy, particularly immune checkpoint inhibitors. Finally, we found that depletion of ATP2A1, one of the risk genes in our model, influence the biologic behaviour of CRC cells significantly.Conclusion: The m7G-related DDR genes can be used as important markers for predicting patient prognosis and immunotherapy response. Our data suggest that ATP2A1 may promote the proliferation of colon cancer cells. These findings may provide new therapeutic targets for the treatment of colon cancer.
CMOS-MEMS Thermal-Piezoresistive Resonators and Oscillators for Sensors
Authors: Zope Anurag , Li Sheng-Shian 
Published: June 2022

Microelectromechanical systems (MEMS) are in widespread commercial use due to their compact size, high performance, and low cost. MEMS resonators have emerged as front runners for sensing (accelerometers, gyroscopes, and particulate matter) and frequency (RF front-end, filters, timing, and frequency source) applications. The excellent stability, resolution, and accuracy of resonators lead them an ideal candidate for sensor implementation. The CMOS-MEMS technology allows for rapid, large-scale, and low-cost manufacturing. Thermal–piezoresistive resonators (TPRs) are promising candidates due to their favorable potential with scaling and robust performance in the ambient environment. A detailed finite element method (FEM) simulation flow is presented along with a mathematical model for device optimization. The devices were fabricated with the commercial CMOS technology utilizing the front-end-of-line (FEOL) polysilicon and back-end-of-line (BEOL) materials like silicon dioxide and interconnect metal. The flexibility of selective material placement in layout and complex routing using multi-metal interconnect is employed to develop a balanced TPR design at 2 MHz. A 5-MHz bulk mode TPR was designed for mass sensing application. The fabricated devices were characterized, and their performance is compared with other state-of-the-art works. Finally, the developed devices were used in real-world applications for mass sensing and pressure sensing. The device achieved 20 kHz/ng. The TPR devices combine principles of Pirani gauge and resonant sensors for improving the sensing range from 2 to 760 Torr (1 atm).