bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcription factor Foxp1 stimulates angiogenesis in adult rat after myocardial infarction
Authors: Wang Dinghui , Liu Bin , Xiong Tianhua , Yu Wenlong , Yang Huiping , Wang Jing , Jing Xiaodong , She Qiang 
Published: April 2022

Abstract
Background: Coronary heart disease is the leading cause of death and major socioeconomic burden worldwide. The ischemia and hypoxia caused by the sudden occlusion of the main coronary arteries lead to myocardial infarction, leading to hypertrophic growth of cardiomyocytes and left ventricular dilatation, which eventually develops into heart failure. Timely and effective revascularization is extremely important for saving the infarcted myocardium and improving heart function.Purpose: To explore the effect of transcription factor Foxp1 on angiogenesis after myocardial infarction from two levels of in vivo and in vitro experiments.Methods: The myocardial infarction model was established in male SD rats to detect FoxP1 expression; lentivirus carrying FoxP1 interfering RNA was injected by multi-point method for 4 weeks, and tissue immunofluorescence and immunohistochemical experiments were performed to observe myocardial endothelial cell proliferation and collagen deposition. Human umbilical vein endothelial cells (HUVEC) were cultured in a hypoxic cell incubator to detect the expression changes of FoxP1 transcription level and protein level at different time points; Lentiviruses carrying specific FoxP1 gene interfering RNAs were used to transfect HUVECs, and cells were used for transfection. Immunofluorescence and flow cytometry were used to detect the proliferation ability of HUVEC in different groups; meanwhile, cell scratch assay was used to detect the migration ability of HUVEC of different groups and tubule formation experiment to observe the tube formation ability.Results: The myocardial infarction rat model was successfully established. Compared with the sham-operated group, immunofluorescence experiments showed that the expression of FoxP1 was significantly increased. After the lentivirus of FoxP1 interfering RNA was injected into myocardial infarction rats, the expression of FoxP1 decreased, endothelial cell proliferation decreased, and collagen deposition increased. Angiogenesis is reduced. When HUVECs were treated in a 5% hypoxia incubator, it was found that the expression of FoxP1 in endothelial cells increased within 24 hours. After transfection of HUVECs with FoxP1 interfering RNA lentivirus, their proliferation, migration and tube formation abilities decreased significantly.Conclusions: Hypoxia can promote the expression level of endothelial cells FoxP1 to a certain extent; foxp1 may promote the formation of new blood vessels in the infarct area by promoting endothelial cell proliferation and inhibiting endothelial cell apoptosis after myocardial infarction. FoxP1 may provide a promising therapeutic target by promoting effective angiogenesis in the treatment of myocardial infarction.
Evaluating the Spidey sense: The metacognition of intuition
Authors: Lufityanto Galang , Pearson Joel 
Published: April 2022

Abstract
The notion of intuition is a common phenomenological description of how people feel they make some decisions. Although previous work has demonstrated an objective lab-based method to measure intuition, it remains unknown how such objective data might be related to real-world phenomenology of intuition, such as metacognition. To investigate this, we compared performance in an objective lab-based intuition task and its physiological responses with off-line self-report measures of intuitive thinking. We found that intuitive phenomenology was positively correlated with objective intuition and physiology in the lab-based task. Further, we found that those categorized as ‘intuitive thinkers’ performed better on the lab-based intuition task, relative to ‘rational thinkers’. Interestingly, individuals categorized as neither intuitive nor rational thinkers demonstrated decisional adaptability in their degree of intuition, depending on the availability of conscious evidence. These findings support a link between objective psychophysical lab-based measures, physiology, and subjective phenomenology of intuition in decision making: good metacognition.
Production of limonene from textile and cardboard wastes establishes a proof of concept for the sustainable production of terpenes.
Authors: Zebec Žiga , Poberžnik Mojca , Scrutton Nigel S. , Lobnik Aleksandra 
Published: April 2022

Abstract
Cellulose containing textiles (cotton) and cardboard/carton-based wastes represent a large pool of untapped organic carbon. These wastes have enormous potential for utilization as carbon feedstocks in industrial biotechnology processes. Essentially, cotton/cardboard (CC) waste is pure cellulose (with some additives) in the form of polymerised glucose composed of β (1→4) linked D-glucose subunits. CC waste materials can be enzymatically hydrolysed to monomers of D-glucose in aqueous solution, to form ‘glucose juice’, which can then be fermented to bio-ethanol. In principle, value added chemicals could also be produced from glucose juice. Bioconversion of CC waste to other compounds of importance to industry could form the basis of environmentally attractive production routes. Terpenes are one of the largest and most diverse classes of natural compounds, and are of value to industry as they have a wide range of applications as flavourings, fragrances, pharmaceuticals, bio-pesticides and biofuels. Here, we investigated the bioconversion of CC waste to the exemplar terpene limonene as a proof-of-concept. Six different CC waste streams were enzymatically hydrolysed to glucose juice and used to produce limonene by fermentation of an engineered E. coli microbial cell factory. D-glucose content was determined in the glucose juice, and then metabolized by E. coli using an engineered heterogenic biolipid synthesis pathway (the mevalonate pathway) to produce limonene (300–700 mg L− 1), which was then partitioned into a nonane overlay. This study establishes an important proof-of-concept for terpene production from hydrolysed CC waste streams.
Characterization of Hippocampal Synaptic Plasticity in Orchidectomized Insulin-resistant Rats
Authors: Akinbampe Monsurat A. , Abayomi Taiwo Adekemi , Opuwari C. S , Falana Benedict 
Published: April 2022

Abstract
BackgroundAndrogen deprivation can be achieved through testosterone antagonists (chemical castration) with or without orchidectomy. There is this hypothesis that stipulates that testesterone exerts supreme effects on synaptic plasticity, however low testosterone has been linked to type- 2 diabetics mellitus, insulin resistance and Alzheimer’s disease. The aim of the study was to investigate the cellular changes that occur from bilateral orchidectomy and examine the effects of androgen deprivation, orchidectomy and flutamide administration on the histoarchitectural organization of the hippocampus in male Wistar rats. MethodologyThirty-six (36) adult male Wistar rats were randomly divided into into six (6) groups: Control group (distilled water),orchidectomy group (bilateral orchidectomy), flutamide group (oral 10mg,20mg), orchidectomy+flutamide. Animals were sacrificed at 30 days respectively. ResultsThe total plasma; testesterone, insulin levels, fasting blood glucose, and nitric oxide were assayed; the homeostasis model for insulin resistance was also calculated. Histological examinations by Hematoxylin and Eosin(H&E) and Cresyl fast violet (CFV), while immunohistochemical analysis of astrocytes were performed using Glial fibrillary acidic protein (GFAP). Results of study show that androgen deprived insulin-resistance state primarily affects fasting blood glucose and lead to increases in the level of serum insulin, glucose and nitric oxide and caused a decrease in serum testosterone levels. The hippocampal response to flutamide was unaffected by blockade of intracerebral estrogen biosynthesis. In comparison flutamide alone increased CA1 spine synapse density also whereas in combination the effects of flutamide+orchidectomy were additive rather than inhibitory. ConclusionHistopathological results showed that flutamide significantly restored the histological damage of rat brain in flutamide treatment+ orchidectomy.
Enhancing classification performance of binary class imbalanced Data for Weather Forecasting using Machine Learning Classifiers
Authors: Gupta Rahul , Yadav Anil Kumar , Jha SK , Pathak Pawan Kumar 
Published: April 2022

Abstract
Drastic change in climatic conditions is a very big and challenging task for people around the globe. Most of the biological, constructional, transportation and agricultural sectors get affected due to uneven weather conditions, i.e. flood, rainfall, drought, etc. As part of the weather system, rainfall being most prominent phenomena, its rate is treated as one of the most important variables. Meteorological scientists try to identify the parameters of the atmosphere such as temperature, sunshine, cloudiness and humidity of the earth by applying conventional techniques and developing a prediction model. These days, Machine Learning (ML) techniques are more evolving and give more accurate results than the traditional approaches. ML is a subset of artificial intelligence (AI) which is used in this paper for predicting the next day's rainfall from the past 10 year’s weather dataset of Australia. This paper presents the ML classifiers such as Logistic Regression (LR), Decision Tree (DT), Random Forest (RF), Light Gradient Boost Machine (LGBM), Cat Boost (CB), and Extreme Gradient Boost (XGB) to predict the rainfall of the next day. The Python software package having an inbuilt library like Pandas, Numpy Scikitlearn, and Matplotlib is extensively used for data management, mathematical computation, ML modeling, and visualization tools, respectively. This is followed by sequential stages of data visualization, training, testing, modeling, and cross-validation. The evaluation metrics like Area under the Receiver Operating Characteristic (AUROC) curve, recall, accuracy, precision, and Cohen kappa are used to check the performance of ML algorithms.
The Evolution of the COVID-19 Pandemic Through the Lens of Google Searches
Authors: Marty Robert , Maqueda Manuel Ramos , Khan Nausheen , Reichert Arndt 
Published: April 2022

Abstract
Real-time data is essential for policymakers to adapt to a rapidly evolving situation like the COVID-19 pandemic. Relying on Google search interest data across 207 countries and territories, we demonstrate the capacity of publicly-available, real-time data to anticipate COVID-19 cases; evaluate the economic, mental health, and social impacts of containment policies; and identify demand for (mis)information about COVID-19 vaccines. We show that: (1) search interest in COVID-specific symptoms can anticipate rising COVID-19 cases across both high- and low-income settings; (2) countries with more restrictive containment policies experienced larger socio-economic externalities; in addition, lower-income countries experienced less searches for unemployment, but more pronounced mental health externalities; and (3) high vaccination rates are associated with strong demand for information about vaccine appointments and side effects; in some settings, high interest in misinformation search terms is associated with low vaccination rates. Overall, the results demonstrate that real-time search interest data can be a valuable tool for both high- and low-income countries to inform policies across multiple stages of the pandemic.
Computed tomography evaluation of skeletal muscle quality and quantity in morbidly obese individuals with and without metabolic abnormality
Authors: Kwon Soon Hyo , Oh Eunsun , Cho Nam-Jun , Kang Heemin , Kim Sang Hyun , Park Hyeong Kyu 
Published: April 2022

Abstract
We investigated the differences in quantity and quality of skeletal muscle between metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) individuals using abdominal CT. One hundred and seventy-two morbidly obese individuals who underwent bariatric surgery and 64 healthy control individuals participated in this retrospective study. We divided the obese individuals into an MHO and MUO group. CT evaluation of muscle quantity (at the level of the third lumbar vertebra [L3]) was performed by calculating muscle cross-sectional area (CSA), which was normalized to patient height to produce skeletal muscle index (SMI). Muscle quality was assessed as skeletal muscle density (SMD), which was calculated from CT muscle attenuation. To characterize intramuscular composition, muscle attenuation was classified into three categories using Hounsfield unit (HU) thresholds: -190 HU to -30 HU for intermuscular adipose tissue (IMAT), -29 to + 29 HU for low attenuation muscle (LAM), and + 30 to + 150 HU for normal attenuation muscle (NAM). Morbidly obese individuals comprised 24 (14%) MHO individuals and 148 (86%) MUO individuals. The mean age of the participants was 39.7 ± 12.5 years, and 154 (65%) participants were women. MUO individuals had a significantly greater total skeletal muscle CSA than MHO individuals in the model that adjusted for all variables. Total skeletal muscle SMI, SMD, NAM index, LAM index, and IMAT index did not differ between MHO and MUO individuals for all adjusted models. Total skeletal muscle at the L3 level was not different in muscle quantity, quality, or intramuscular composition between the MHO and MUO individuals, based on CT evaluation.
The Association of FKBP5 Gene Methylation, Adolescents’ Sex, and Depressive Symptoms among Chinese Adolescents: A Nested Case-Control Study
Authors: Li Wenyan , Wang Wanxin , Lai Wenjian , Li Xiuwen , Zhu Liwan , Shi Jingman , Teopiz Kayla M. , McIntyre Roger S. , Guo Lan , Lu Ciyong 
Published: April 2022

Abstract
Background:Depressive symptoms among adolescents are a serious health concern around the world. Altered DNA methylation in the FK506 binding protein 5 (FKBP5) gene has been reported to regulate stress response, which has been reported to be closely associated with depressive symptoms. However, most of the contributing studies have been conducted among adults and relatively few studies have considered the effect of disparate social influences and sex differences on the DNA methylation of FKBP5 in persons with depressive symptoms. The present study aimed to test the associations of FKBP5 DNA methylation and depressive symptoms among adolescents and explore possible sex differences in the foregoing associations.Methods:This study was conducted using a nested case-control design within a longitudinal cohort study from January 2019 to December 2019. Adolescents aged 12 to 17 years from 69 classes in 10 public high schools located in Guangdong province of China participated in this research. Students with depressive symptoms at baseline and follow-up were randomly selected as the case group, and those without depressive symptoms were treated as the control group. Our study finally included 87 cases and 151 controls matched for age.Results:The overall DNA methylation trend of FKBP5 CpG sites in the case group was lower in comparison to the control group. Compared to healthy controls, lower methylation percentage of FKBP5-12 CpG 1 was observed in adolescents with depressive symptoms after adjusting for covariates (case: 0.94 ± 2.00, control: 0.47 ± 0.92; F = 5.41, P = 0.021). However, the hypomethylation of FKBP5-12 CpG 1 was approaching significance after adjustment for social-environmental factors (aOR = 0.77; P = 0.055), which indicated that hypomethylation of FKBP5 CpG sites may not be an independent risk factor for depressive symptoms in our sample. In addition, in the present study, we were unable to identify sex differences in the association of FKBP5 gene methylation with depressive symptoms.Conclusions:The decreased methylation level of FKBP5 was observed in adolescents with depressive symptoms. Our results presented here are preliminary and underscore the complex gene-environment interactions relevant to the risk for depressive symptoms.
Performance Evaluation of Free Hole Transport Layer CsPbI3 Perovskite Solar cells
Authors: AbdElAziz Hadeer 
Published: April 2022

Abstract
Full inorganic cesium lead iodide (CsPbI3) HTL free transport layer is investigated and simulated using SCAPS. The aim of the study is to investigate the optimum performance of the device structure. Using DFT study, the optical refractive index and reflectivity of CsPbI3 are calculated. Further investigation of the device performance with changing parameters like metal back contact, absorber thickness, acceptor density, and defect density. The solar cell device has the optimum performance with Selenium as a back contact. The solar cell device structure of (FTO/ZnO/CsPbI3/Se) with Voc of 1.34 V, Jsc of 19.75 mA/cm2, an FF of 87.5%, and PCE of 23.25% are acquired for the proposed HTL-free CsPbI3-based PSC. The simulation study will be a guide in fabricating low cost highly efficient HTL free inorganic CsPbI3 solar cells.
Long-term nitrogen and phosphorus additions change community diversity of arbuscular mycorrhizal fungi and crop yield are mainly determined by Glomus and Paraglomus in the Loess Plateau
Authors: Li Chunyue , Zhang Wenting , Wang Yi , Miao Yu , Chang Shun , Kou Zhaoyang , Li Qianxue , Dang Tinghui 
Published: April 2022

Abstract
Aims
Arbuscular mycorrhizal fungi (AMF) can form mutualistic symbiosis with more than 80% of plant roots on land, playing an important role in promoting plant growth. Similarly, nitrogen (N) and phosphorus (P) addition, a common fertilization practice in the Loess Plateau, China, can improve crop yield, and further has an important effect on soil microbial community. However, the potential effects of long-term N and P addition on AMF community structure in farmland system in loess arid region are still unclear.
Methods
Based on the Changwu Agricultural Ecology Experimental Station of the Chinese Academy of Sciences, this study set three N and P concentration gradients: low (0 kg hm− 2), medium (90 kg hm− 2) and high (180 kg hm− 2), and carried out a 3 × 3 complete interaction experiment with 9 treatments, which were CK, N12, N24, P12, N12P12, N24P12, P24, N12P24, N24P24. In this study, we combined high-throughput sequencing technology with traditional biochemical methods to analyze the composition and structural diversity of AMF community. Besides, correlation analysis between AMF community composition, soil environmental factors and crop yield was also preformed.
Results
Our results showed that AMF community diversity increased significantly under the N and P interaction. The AMF community composition was differentiated by N and P additions across the concentration gradients, driven mostly by the available phosphorus (AP), total phosphorus (TP) and pH as the main environmental factors. Correlation analysis revealed a significant positive correlation between Glomus and wheat yield, Paraglomus opposite.
Conclusions
Long-term N and P addition not only directly increased the crop yield, but also affected wheat yield indirectly by affecting soil physicochemical properties and AMF community diversity. Rational N and P application could improve the ecological and physiological functions of AMF community, which was of positive significance for improving crop yield and agricultural sustainable development.
Life Story Book to Enhance Communication in People with Dementia: A Systematic Review of Reviews
Authors: Subramaniam Ponnusamy , Thillainathan Preyaangka , Ghani Nur Amirah Mat , Sharma Shobha 
Published: April 2022

Abstract
Background: Life Story Book (LSB) has been commonly used in promoting person-centred care in elderly care especially for people with dementia. This involves collecting the life stories and memories and then being compiled to produce a tangible output, usually a book often termed the Life Story Book. However, the use, benefits and the influences of Life Story Book in dementia care is still scarce and requires an overall review. The aim of this systematic review is to collect past reviews and provide a thorough overview on the use, benefits, and impact of the Life Story Book for the person with dementia, the relatives, family and caregivers. Methods: The electronic databases PubMed, Scopus, Science Direct and Web of Science as well as grey literature through Google Scholar were searched to select the relevant studies. Results: Seven studies that meet the inclusion criteria were selected and data was synthesised. Findings revealed that the use of the Life Story Book has no specific guidelines and has been described with numerous characteristics and varied methods of implementation. The Life Story Book intervention is found to provide significant positive outcomes for the person with dementia and the carers involved. Six out of the seven studies reported that Life Story Book enhanced communication between persons with dementia, relatives, care staff and residents. Conclusion: The review extends the current evidence on the usage of the Life Story Book in dementia care and confirms that the use of life stories leads to better care in various setting. However, more research is needed to reveal the potential of Life Story Book in enhancing communication and the guideline or training for staff and carers to deliver the intervention.
Prognostic implications of MUC1 and XBP1 concordant expression in multiple myeloma: A retrospective study
Authors: Rajput Sheerien , Minhas Khurram , Azam Iqbal , Shaikh Usman , Lalani El-Nasir 
Published: April 2022

Abstract
Background:Multiple myeloma (MM) is a disease of malignant plasma cells (PC) with poor survival. Disease progression and treatment relapse are attributed to MM cancer stem cells (CSCs) and signaling molecules such as MUC1 and XBP1. The prognostic value of the expression of CSC associated biomarkers, MUC1 and XBP1 in MM, has not been explored previouslyMethod:In this study, we determined the immunohistochemical expression of CSC markers (ALDH1, CD117 and CD34), MUC1 and XBP1in 128 MM formalin-fixed paraffin-embedded bone marrow archival blocks. The expression of biomarkers was assessed for association with clinicopathological variables and the overall survival of patients. Descriptive & initial univariate survival analyses were performed using Kaplan Meier curves. Univariate and multivariable analyses were performed using simple and multiple Cox regression models. The results are reported as crude & adjusted hazard ratios with 95% confidence intervals. Results :Expression of ALDH1 and CD117 was found in 51% and 48% of the tumors, respectively. ALDH1 expression was associated with 1.83 years of reduced survival for patients with CD56 negative tumors. MUC1 expression was observed in 62%, whereas XBP1 was expressed in 48% tumors. Combinatorial group analysis of XBP1 and MUC1 stratified patients into two prognostic groups. Cases with tumors negative for expression of MUC1 and XBP1 (XBP1- / MUC1-) were categorized as a good prognostic group with increased survival of 3.42 years compared to cases with tumors expressing both (Worst prognosis, XBP1+/MUC1+). The adjusted hazard ratio showed a four-fold increased risk of mortality associated with the concordant expression of MUC1 and XBP1 in patients who were >65 years of ageConclusion: Concordant expression of MUC1 and XBP1 in MM defines a subset of patients with adverse outcomes. Of these, patients diagnosed at >65 years of age have a significantly higher risk of death.