bluuebunny/crossref_metadata_2025_split huggingface dataset:
The change of microglia numbers within the mice retina, optic nerve and chiasm following intravitreal AAV2-GFP injection
Authors: Ji Yuanfei , Yu Bo , Zhang Yikui , Wu Wencan 
Published: December 2021

Purpose To explore the optimized concentration of AAV2-GFP for sparse transfection of retinal ganglion cells (RGCs) and optic nerve (ON), and to examine the changes of microglial morphology and distribution in the retina, optic nerve and chiasm after injection. Methods We defined the optimal concentration of AAV2-GFP for sparse labeling of RGCs and axons in WT mice. We further explored the changes of microglial morphology and distribution in the retina, optic nerve and chiasm after intravitreal injection in CX3CR1+/GFP mice. Results 14 days after intravitreal injection of AAV2-GFP, live imaging of the retina showed that fundus fluorescence was very strong and dense at 2.16 × 1011 VG/retina, 2.16 × 1010 VG/retina, 2.16 × 109 VG/retina. RGCs were sparsely marked at a concentration 1:1000 (2.16 × 108 VG/retina) and fundus fluorescence was weak. The transfected RGCs and axons were unevenly distributed in the retina and significantly more RGCs were transfected near the injection site of AAV2-GFP compared to the other sites of the flat-mounted retina. Microglia density increased significantly in the retina and part of optic nerve, but not in the optic chiasm. The morphology of microglia was largely unchanged. Conclusions AAV2-GFP was highly efficient and the optimal concentration of sparsely labeled RGCs was 1:1000 (2.16 × 108 VG/retina). After intravitreal injection of AAV2-GFP, the number of microglia increased partly. The morphology of microglia was comparable.
A single-centre, retrospective analysis of mortality over 80 months comparing paclitaxel-coated balloon versus standard balloon angioplasty in the treatment of dysfunctional arteriovenous access
Authors: Riding Alexandra M , Al-Nowfal Ahmed , Ramanarayanan Siva , Swift Oscar , Mathavakkannan Suresh , Jeevaratnam Praveen , Steiner Kate 
Published: December 2021

Aim: Percutaneous transluminal angioplasty (PTA) is a standard treatment for arteriovenous fistula (AVF) stenosis to preserve haemodialysis vascular access, promoting improved dialysis adequacy and better outcomes for those dependent on renal replacement therapy. Drug coated balloons (DCB) may help reduce the rate of neointimal hyperplasia and recurrent stenosis, but their use in femoropopliteal angioplasty has been associated with increased mortality at 2 and 5 year follow-up. This study aims to address the long-term safety of PTA for AVF stenosis with clinical correlation to participant co-morbidity and mortality. Methods: All patients undergoing PTA for AVF stenosis at a single centre between 2013 and 2017 were identified and grouped according to the use of DCB versus standard balloon angioplasty. All data was anonymised and correlated to verify independent predictors of mortality. Results: 481 (400 standard balloon; 81 DCB) procedures were performed in 313 patients (250 standard balloon; 63 DCB). Follow-up at 80 months did not show any difference in mortality ( p = 0.546). Multivariate analysis identified time on dialysis ( p < 0.001), age ( p = 0.001) and Charlson comorbidity index ( p = 0.02) as independent predictors of mortality. Conclusions: In this study, mortality was not associated with the use of DCBs, but was related to established factors of dialysis longevity, age and comorbidity.
Plastic cannulae versus metal needle cannulation in haemodialysis: Results of an international survey from the nurse perspective
Authors: de Barbieri Ilaria , Sisti Davide , Strini Veronica , Noble Helen , Parisotto Maria Teresa , Amatori Stefano , Rocchi Marco BL 
Published: December 2021

Background: In haemodialysis is key to successfully obtaining cannulation of the arteriovenous fistula (AVF). The literature agrees that cannulation, failing in the initial maturation period, can lead to delayed dialysis, haematoma, scarring, needle phobia and loss of confidence in the cannulator. The introduction of plastic cannulae for haemodialysis has changed cannulation practice positively, preventing frequent complications such as infiltration or trauma. Despite that, most countries have continued to use metal cannulation, in particular in Europe. This study investigates the common use of plastic cannulae versus metal needles for cannulation in dialysis units and explores the implications of focusing on the side effects of cannulation. Methods: The study is a cross-sectional survey. A questionnaire was created by a team of experts from the European Dialysis Transplant Nurse Association/European Renal Care Association (EDTNA/ERCA) to address the study's aims and sent online to nurse members. Results: Data collected suggested a strong resistance towards using plastic cannulae, with few respondents claiming to use these cannulas. Most of the respondents were female (74%), Europeans, working in nephrology for more than 10 years and most worked in the public sector. There was a strong correlation between the use of plastic cannulae and fewer adverse events in elbow located AVF and newly created or fragile AVF. Conclusions: The results are in line with the current literature. Possible resistance to the use of the plastic device includes the difference in cost between the two devices in favour of metal needles. However, it should be considered that the lower number of adverse events, in particular infiltration and haematoma caused by the metal needle, involves a considerable saving both in money and in terms of time and distress for the patient.
Ranger™ paclitaxel-coated balloon versus conventional balloon angioplasty for treatment of failing arteriovenous fistulas and grafts in haemodialysis patients: A retrospective cohort study
Authors: Soon Shereen XY , Tan Ru Yu , Pang Suh Chien , Yap Charyl JQ , Patel Ankur , Gogna Apoorva , Tan Chieh Suai , Chong Tze Tec , Tang Tjun Y 
Published: December 2021

Background: Aim was to compare the safety and patency efficacy outcomes between Ranger™ paclitaxel-coated balloon (PCB)- versus conventional balloon angioplasty (POBA) in the treatment of haemodialysis access-related conduit stenosis. Methods: Retrospective single-centre, multi-investigator, consecutive, double-arm comparative cohort study. About 130 end-stage renal failure Asian patients with dysfunctional arteriovenous fistula (AVF) or arteriovenous graft underwent PCB or POBA fistuloplasty between November 2018 and June 2020. All stenotic lesions were prepared with high pressure non-compliant balloon angioplasty prior to PCB angioplasty. All patients received at least one antiplatelet agent for 3 months duration post procedure. Results: Mean age was 66.0 ± 10 years and 79/130 (61%) were males. PCB arm ( n = 65) versus POBA arm ( n = 65). Majority were AVFs circuits (122/130, 94%). Main indication for intervention was dropping access flow (98/130, 76%). About 172 lesions were treated (56% POBA, 44% PCB), and the juxta-anastomosis (JAS) was the main target lesion (87/172, 51%). There were no significant differences in safety outcomes (30-day adverse events, access thrombosis, abandoned AVF and death) between treatment groups. Mean time to target lesion reintervention (TLR) was longer in PCB-treated lesions (7.1 ± 2.7 vs 5.8 ± 3.2 months, p = 0.03), especially amongst recurrent lesions (7.3 ± 2.4 vs 5.7 ± 3.2, p = 0.02). Mean time to circuit reintervention was also longer in PCB-treated circuits (6.9 ± 2.8 vs 5.8 ± 3.7months, p = 0.04). There were 16 deaths (12%), all attributed to patient’s underlying comorbidities. Conclusions: Fistuloplasty with Ranger™ PCB for failing arteriovenous circuits in end-stage renal failure patients, is a safe and efficacious modality compared to POBA in terms of longer freedom from TLR.
Endovascular salvage of failing arterio-venous fistulas utilising sirolimus eluting balloons: Six months results from the ISABELLA trial
Authors: Tang Tjun Y , Soon Shereen XY , Yap Charyl JQ , Tan Ru Yu , Pang Suh Chien , Patel Ankur , Gogna Apoorva , Tan Chieh Suai , Chong Tze Tec 
Published: December 2021

Background: Aim of this pilot clinical study was to evaluate the safety and efficacy of the Selution Sustained Limus Release (SLR)™ sirolimus-eluting balloon (SEB) for improving failing arterio-venous fistulas (AVF) patency in Asian haemodialysis patients. Methods: Prospective single-centre, multi-investigator, non-consecutive, non-blinded single arm trial. Forty end-stage renal failure Asian patients with a dysfunctional AVF underwent SEB angioplasty between May and November 2020. All stenotic lesions were prepared with high pressure non-compliant balloon angioplasty prior to SEB angioplasty. Endpoints of interest included target lesion primary patency and circuit access patency and safety through 30 days. All patients received dual antiplatelet therapy for 1 month and were followed up with Duplex ultrasound at 6 months. Results: There was one subject dropout so final n = 39 patients (mean age 65.0 ± 11.9; males = 26 (66.7%)) and n = 43 target lesions treated. Main indication for intervention was dropping access flow (24/39; 61.5%) and most common target lesion was in the juxta-anastomosis (24/43; 54.5%). There was 100% technical and procedural success. There were no adverse events related to the SEB. Target lesion primary patency rates at 3 and 6 months were 39/41 (95.1%) and 28/39 (71.8%) respectively. Access circuit patency rates at 3 and 6 months were 35/37 (94.6%) and 22/35 (62.9%) respectively. There were 3 (7.7%) deaths all attributable to patients’ underlying co-morbidities. Conclusions: Fistuloplasty using the novel Selution SLR™ SEB for dysfunctional AVF circuits seems a safe and effective modality in Asian haemodialysis patients at 6 months but larger randomised controlled studies are required now to determine its true efficacy against plain balloon angioplasty.
Impact of clinical outcomes and imaging measures on health-related quality of life in secondary progressive MS
Authors: W Koch Marcus , Mostert Jop , Repovic Pavle , Bowen James D , Strijbis Eva , Uitdehaag Bernard , Cutter Gary 
Published: December 2021

Background: Health-related quality of life (HRQOL) outcomes are often included as secondary outcomes in clinical trials in secondary progressive MS (SPMS), but little is known about the longitudinal association of HRQOL and clinical and imaging outcome measures in SPMS. Objective: To assess the association of change in clinical and imaging outcomes with HRQOL in people with SPMS. Methods: We used data from ASCEND, a large randomized controlled trial ( n = 889), to investigate the association of significant worsening on the Expanded Disability Status Scale (EDSS), Timed 25 Foot Walk (T25FW), Nine Hole Peg Test (NHPT), Symbol Digit Modalities Test (SDMT), and change in lesional and volumetric imaging outcomes with significant worsening on the 36-Item Short Form Health Survey (SF-36) and the Multiple Sclerosis Impact Scale (MSIS-29) during 2 years of follow-up using logistic regression models. Results: HRQOL measures were most associated with EDSS and T25FW, less so with NHPT and SDMT, and not associated with lesional and volumetric imaging outcomes. Discussion: Worsening of the EDSS and T25FW was associated with two commonly used HRQOL measures. These outcomes therefore appear to be more patient relevant than either the NHPT or SDMT in the context of a 2-year clinical trial.
The contribute of cerebrospinal fluid free light-chain assay in the diagnosis of multiple sclerosis and other neurological diseases in an Italian multicenter study
Authors: Bernardi Gaetano , Biagioli Tiziana , Malpassi Paola , De Michele Teresa , Vecchio Domizia , Repice Anna Maria , Lugaresi Alessandra , Mirabella Massimiliano , Torri Clerici Valentina , Crespi Ilaria 
Published: December 2021

Background: Cerebrospinal fluid (CSF) free light chains (FLCs) can be an alternative assay to oligoclonal bands (OCBs) in inflammatory neurological disorders, but threshold has no consensus. Objective: To assess the diagnostic accuracy of CSF FLCs in multiple sclerosis (MS) and other neurological diseases. Methods: A total of 406 patients from five Italian centers. FLCs were measured in CSF and serum using Freelite MX assays on Optilite. Results: A total of 171 patients were diagnosed as MS, 154 non-inflammatory neurological diseases, 48 inflammatory central nervous system (CNS) diseases, and 33 peripheral neurological diseases. Both kFLC and λFLC indices were significantly higher in patients with MS compared to other groups ( p < 0.0001). The kFLC index ⩾ 6.4 is comparable to OCB for MS diagnosis (area under the receiver operating characteristic curve (AUC) = 0.876; sensitivity 83.6% vs 84.2%; specificity 88.5% vs 90.6%). λFLC index ⩾ 5 showed an AUC of 0.616, sensitivity of 33.3% and specificity of 90.6%. In all, 12/27 (44.4%) MS patients with negative OCB had kFLC index ⩾ 6.4. Interestingly, 37.5% of 24 patients with a single CSF IgG band showed high kFLC index and 12.5% positive λFLC index. Conclusion: Our findings support the diagnostic utility of FLC indices in MS and other CNS inflammatory disorders, suggesting a combined use of FLC and OCB to help clinicians with complementary information.
Factors associated with initiation and use of snus among adolescents
Authors: Ansteinsson Vibeke , Mdala Ibrahimu , Becher Rune , Grøtvedt Liv , Kopperud Simen E. , Rukke Valen Håkon 
Published: December 2021

Aim: We investigated factors associated with the initiation and continuation of snus use in adolescents in Norway. The associations with adolescents’ own educational plans, the parents’ educational level(s) and tobacco habits were estimated. Methods: In this cross-sectional questionnaire-based study, 1465 patients aged 18–20 years participated. The questionnaire was administered at regular dental examinations in the public dental health service. To assess the association between individual factors and the initiation of tobacco habits, a generalised structural equation model with random effects at the clinic level was used. Binary responses were modelled using multilevel binary logistic regression, while the number of snus boxes used per month was modelled using a multilevel Poisson regression model. Results: Of current (daily and occasional) tobacco users, 85% were snus users, including dual users of both snus and cigarettes. The median age of snus initiation was 16 years. Both parental snus use and smoking were associated with an increased risk of snus initiation, snus use and a higher amount of use. An increased risk of using snus was associated with male gender and with no educational plans or planning for further vocational education. The amount of snus used was higher among current snus users with a prior smoking history and among those planning for further vocational education. Conclusions: These findings may aid in developing and targeting tobacco prevention strategies aimed at young people. Tobacco prevention measures should start at the elementary school level. The strong association with parental tobacco habits underlines the importance of parents’ influence on their children’s tobacco use.
Risk, worry and motivation: How is public knowledge of dementia shaped?
Authors: Ashworth Rosalie , Bassett Zoe , Webb Jake , Savage Sharon 
Published: December 2021

Background and Objectives Due to the concern over global rising rates of dementia, increased emphasis has been placed on understanding and moulding the public’s knowledge and awareness of the condition. There has been limited previous research into predictors of dementia knowledge; overall knowledge amongst the public is low, and it has been widely agreed that more needs to be done to raise awareness of this condition. This study seeks to solidify understanding of public dementia knowledge and introduces dementia worry, motivation to seek information and risk perception as novel concomitants of this knowledge. Research Design and Methods A convenience sample of 311 UK adults completed a survey on dementia knowledge including Alzheimer’s disease-specific questions, worry about developing dementia, motivation to seek information and perceived personal risk of getting the disease. Surveys were completed face-to-face and included both closed and open-ended questions. Results Overall dementia knowledge scores were low, achieving an average of 33% of the total possible score, with 88% of the sample scoring below 50%. Bivariate correlations were performed between dementia knowledge and key variables, revealing significant positive relationships with risk perception ( r = 0.179, p = .002), worry ( r = 0.140, p = .016) and motivation to seek information ( r = 0.139, p = .016). When knowledge was dichotomised into high and low, worry about ( p = .28) and perceived risk ( p = .19) of dementia was significantly lower for people with low knowledge scores than for people with higher dementia knowledge scores. Motivation to seek information was not significantly different between the high and low knowledge groups ( p = .071). Discussion and Implications Despite the relatively low knowledge scores, findings show a positive relationship between modifiable factors and dementia knowledge, suggesting areas to consider for both further research and publication campaigns. Further implications and limitations of this study are discussed.
Effects of drilling parameters on delamination of kenaf-glass fibre reinforced unsaturated polyester composites
Authors: Ngah AR , Salman Suhad D , Leman Z , Sapuan SM , Alkbir MFM , Januddi Fatihhi 
Published: December 2021

Drilling is a secondary material removal and usually carried out to facilitate fastening of parts together. Drilling of composite materials is not usually a problem-free process. Issues related to delamination composite laminates need to be addressed because it introduces the stress concentration point on the composite. This study focussed on the influence of process parameters such as spindle speed, feed rate, type of drill bits and geometry on the extend of delamination experienced by the composite during the drilling process of kenaf-glass fibre-reinforced unsaturated polyester composite, and the delamination measurements were taken under a microscope. Taguchi methods and analysis of variance were employed to find the optimal parameters. From the results, the most significant parameter was the feed rate. The minimum delamination was achieved when the feed rate was 0.05 mm/rev and spindle speed was 700r/min using both types of drill bits. The quality of the drill hole using the twist drill bit has been proven to be better than the brad drill bit.
An artificial neural network prediction on physical, mechanical, and thermal characteristics of giant reed fiber reinforced polyethylene terephthalate composite
Authors: Gulihonenahali Rajkumar Arpitha , Hemath Mohit , Kurki Nagaraja Bharath , Neerakallu Shivakumar , Thiagamani Senthil Muthu Kumar , Asrofi Mochamad 
Published: December 2021

Plant fiber reinforced hybrid polymer composites have had broad applications recently because of their lower cost advantages, lower weight, and biodegradable nature. The present work studies the influence of reinforcing giant reed fiber concentration in polyethylene terephthalate (PET) polymer for their physical, mechanical, and thermal characteristics and determines the optimum loading of giant reed fiber using an artificial neural network (ANN) scheme. Giant reed fiber reinforced PET matrix laminates were manufactured from compression molding with different fiber loadings such as 5 wt.%, 10 wt.%, and 20 wt.%. The mechanical characteristics such as tensile and flexural strength and the laminate’s tensile and flexural modulus were appraised and examined. The maximum value of tensile strength, flexural strength, tensile modulus, and flexural modulus were 5.4 MPa, 26 MPa, 8343 MPa, and 6300 MPa, respectively, for PET2 (10 wt.% of giant reed fiber in PET polymer) composite. Fiber pullout, gaps, and fracture behavior were examined from a scanning electron microscope in the microstructural analysis. A machine learning technique has been recommended to combine artificial intelligence while designing giant reed fiber reinforced polymeric laminates. Using the suggested method, an ANN model has been generated to attain the targeted giant reed fiber concentration for PET composite while gratifying the necessary targeted characteristics. The developed method is very effective and decreases the effort and time of material characterization for huge specimens. It will support the researchers in designing their forthcoming test efficiently.
Improvement in Emotion Regulation While Detained Predicts Lower Juvenile Recidivism
Authors: Docherty Meagan , Lieman Andrew , Gordon Brandon Lee 
Published: December 2021

The goal of the current study was to investigate the relationships between observer-rated skills related to emotional and cognitive regulation post-admission and pre-release in a secure facility and official records of juvenile felony recidivism up to 1 year after release. Data came from a sample of 599 youth in a residential facility in Washington state (84% male; 38% White). Latent change score models indicated that both initial level of emotional regulation skills and improvement in emotion regulation skills while incarcerated were significantly related to lower recidivism. This pattern of findings remained when controlling for length of stay, among other covariates. Follow-up analyses indicated that the results for emotion regulation skills might be driven primarily by monitoring internal and external triggers. Additional research should investigate the connection between emotion regulation skills and juvenile recidivism, with a special focus on trigger monitoring and how to improve those skills.
Clinical impact of Sim & Size® simulation software in the treatment of patients with cerebral aneurysms with flow-diverter Pipeline stents
Authors: Mantilla Daniel , Ferreira-Prada Carlos A , Galvis Melquisedec , Vargas Oliverio , Valenzuela-Santos Carolina , Canci Piergiorgio , Ochoa Miguel , Nicoud Franck , Costalat Vincent 
Published: December 2021

Objectives This study evaluated the clinical impact of the Sim&Size® simulation software on the endovascular treatment with flow-diverter stents of patients with unruptured saccular intracranial aneurysms. Methods This monocentric retrospective study evaluated a cohort of patients treated with flow-divert stents between June 1, 2014, and December 31, 2019, for cerebral aneurysms. Patients belonged to two groups, patients treated with and without the Sim&Size® simulation software. Univariate, bivariate, and multivariate analyses were used to evaluate the clinical impact of simulation software. Results Out of the 73 interventions involving 68 patients analyzed by the study, 76.7% were simulated using the Sim&Size® simulation software, and 23.3% were not. Patients treated with the simulation software had shorter stent lengths (16.00 mm vs. 20.00 mm p-value = 0.001) and surgical time (100.00 min vs. 118.00 min p-value = 0.496). Also, fewer of them required more than one stent (3.6% vs. 17.6% p-value = 0.079). Three patients belonging to the non-stimulated group presented hemorrhagic complications. Conclusions Using the Sim&Size® simulation software for the endovascular treatment of intracranial aneurysms with pipeline flow-diverter stents reduces the stent length.