bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design and development of a self-assembling protein nanoparticle displaying PfHAP2 antigenic determinants recognized by natural acquired antibodies
Authors: Zahedi Farhad , Abouie Mehrizi Akram , Sardari Soroush , Alemzadeh Iran 
Published: September 2022

Backgrounds
In order to move towards the elimination and eradication of malaria in the world, the development of vaccines is inevitable. Many modern vaccines are based on recombinant technology; however, they may not provide a fully protective, long-lasting immune response. One of the strategies to improve recombinant vaccines is designing the nanovaccines such as self-assembling protein nanoparticles (SAPNs). Hence, the presentation of epitopes in a repeat array and correct conformation should be considered. P. falciparum generative cell-specific 1 (PfGCS1) is a main transmission-blocking vaccine candidate with two highly conserved fragments, HAP2-GCS1 and cd loop, inducing partial malaria transmission inhibitory antibodies. Therefore, to design an effective malaria vaccine, we used cd loop and HAP2-GCS1 fragments at the amino and carboxy terminuses of the SAPN-forming amino acid sequence, respectively.

Methodology/Principal findings
The SAPN monomer (PfGCS1-SAPN) sequence was designed, and the three-dimensional (3D) structure was predicted. The result of this prediction ensured the presence of antigens on the SAPN surface. Then the accuracy of the predicted 3D structure and its stability were confirmed by 100 ns molecular dynamics (MD) simulation. The designed SAPN substructure sequence was synthesized, cloned, and expressed in Escherichia coli. With a gradual decrease in urea concentration in dialysis solutions, the purified proteins progressed to the final desired structure of the SAPN, which then was confirmed by Dynamic Light Scattering (DLS) and Field Emission Scanning Electron Microscopy (FESEM) tests. According to the Enzyme-Linked Immunosorbent Assay (ELISA), antigenic determinants were presented on the SAPN surface and interacted with antibodies in the serum of malaria patients.

Conclusions/Significance
Our results show that the SAPN formed by PfGCS1-SAPN has produced the correct shape and size, and the antigenic determinants are presented on the surface of the SAPN, which indicates that the designed SAPN has great potential to be used in the future as a malaria vaccine.
Disability-adjusted life years (DALYs) based COVID-19 health impact assessment: A systematic review protocol
Authors: Gebeyehu Daniel Teshome , East Leah , Wark Stuart , Islam Md Shahidul 
Published: September 2022

Background
COVID-19 is a highly contagious infectious disease that emerged in 2019. This disease is causing devastating health, socio-economic, and economic crises. More specifically COVID-19 is affecting both the quality and length of human life. The overall health impact of this disease is measured by the disability-adjusted life years which is the sum of the life years lost due to disability (the effect on the health quality) and the years life lost due to premature death (effect on the length of life). The purpose of this review is to summarise DALYs-based health impact publications and produce compiled and informative literature that can aid the health regulators to make evidence-based decisions on mitigating COVID-19.

Methods
The review will be conducted using the PRISMA 2020 guidelines. The DALYs-based original observational and cross-sectional studies will be collected for assessing the health impact of COVID-19. Both the life quality and length impacts of COVID-19 will be reviewed. The life quality impact of COVID-19 will be measured using the life years lost due to disability (pre-recovery illness, pre-death illness, and post-acute consequences), and its impact on the length of life will be measured with years of life lost due to premature death (shortening of life expectancy). The combined health impact of COVID-19 on the quality and length of life will be measured in disability-adjusted life years.

Discussion
The impacts of COVID-19 on the two health outcomes (quality and length of life) will indicate the level of COVID-19 health burden. The increase or decrease of COVID-19 health impact might be due to the sample size differences of different studies and the omission of years lost due to post-acute consequences in some studies. After having a summarized systematic review health decision-makers will apply an impact-based response to COVID-19.

Trail registration
Systematic review registration: This protocol is pre-registered in PROSPERO with the registration number CRD42022324931.
Time to doubling of serum creatinine in patients with diabetes in Ethiopian University Hospital: Retrospective follow-up study
Authors: Netere Adeladlew Kassie , Sendekie Ashenafi Kibret 
Published: September 2022

Background
Diabetic kidney disease is one of the long-term microvascular complications of diabetes. Doubling of serum creatinine is an important biomarker and predictor of diabetic kidney disease for patients with diabetes. This study aimed to determine the time in which the serum creatinine level is doubled measured from the baseline in patients with diabetes in Ethiopian University Hospital.

Methods
Analysis of the patients with diabetes medical records was employed retrospectively for five years from 2016 to 2020 in the University of Gondar Comprehensive Specialized Hospital. The Kaplan-Meier procedure was used to predict the time to which the serum creatinine level was doubled measured from the baseline value, while the Log-rank test and cox-proportional hazard regression models were employed to show significant serum creatinine (SCr) changes against the predictor variables.

Results
Among the total of 387 patients with diabetes, 54.5% were females with a mean age of 61.1±10.3 years. After 5-years of retrospective follow-up, 10.3% of patients with diabetes had doubled their serum creatinine level computed from the baseline values. The baseline and last SCr levels (measured in mg/dL) were 0.87 (±0.23) and 1.0(±0.37), respectively. This resulted in a mean SCr difference of 0.12±0.38 mg/dL. The SCr score was continuously increasing uninterruptedly for five years and measured as 0.94, 0.95, 0.94, 1 and 1.03 mg/dL, respectively. The average survival time taken for the serum creatinine to be doubled computed from baseline was 55.4 months (4.6 years). Patients treated with greater than or equal to 30 IU NPH were found 3.3 times more likely to have higher risks of doubling the serum creatinine level (DSC); with HR of 3.29 [(95%CI); 1.28–8.44: P = 0.013].

Conclusion
Compared with the baseline level, a significant proportion of patients with diabetes were found to have doubling of serum creatinine DSC within less than five years around four and half years. A continuous increasing in the SCr level was noted when measured from the baseline scores. Therefore, to preserve the renal function of patients with diabetes, close SCr level monitoring and regular follow-up would be recommended in combined with effective therapeutic interventions.
construção de um banco de dados lexicográfico em XML a partir de dados dialetais
Authors: Santos Junior Jorge Luiz Nunes dos , Isquerdo Aparecida Negri 
Published: September 2022

Este artigo situa-se na interface entre a Lexicografia (PORTO DAPENA, 2002; HARTMANN, 2016), a Dialetologia (CARDOSO, 2010; CHAMBERS; THUDGILL, 1994) e a Linguística Computacional (HABERT, 2004; PÉREZ HERNÁNDEZ; MORENO ORTIZ, 2009; HAUSSER, 2014; KURDI, 2016). Objetiva-se discutir a proposta de construção de um banco de dados em XML (Extensible Markup Language), explorando os resultados obtidos com o PLN (Processamento Automático de Linguagem Natural). O arquivo XML também se fundamenta em parâmetros da Lexicografia Dialetal (EZQUERRA, 1997; NAVARRO CARRASCO, 1993) e está sendo alimentado com dados dialetais oriundos do Projeto Atlas Linguístico do Brasil (ALiB) documentados na região Norte do país. Para tanto, utilizou-se como editor de texto o software jEdit e, para gerenciar o banco de dados, o programa BaseX. A extração das informações linguísticas foi realizada, no BaseX, a partir de uma amostra de dados e com o auxílio de expressões X-Query. Assim, foram executadas as seguintes manipulações de dados: i) localização de uma unidade lexical específica; ii) visualização de qualquer dado da microestrutura filtrada pelas variáveis sexo, idade, escolaridade e localidade; iii) seleção de informações a partir de uma das 14 áreas semânticas em que as questões do questionário semântico-lexical do ALiB foram organizadas. Em síntese, entende-se que a construção do banco de dados em XML confere agilidade em relação à extração de informações e compatibilidade dos dados para executar interfaces com outras aplicações como, por exemplo, a elaboração de um produto lexicográfico a ser publicado em suporte on-line.
Biological Corridors as Enhancers of Local Development: Case Study of Biological Corridor Alexander Skutch
Authors: Arauz-Beita Ileana , Arias-Navarro Adrián 
Published: September 2022

When we relate to Protected Green Belts they involve a delimited territory, usually privately owned and whose purpose is to provide connectivity between landscapes, ecosystems and habitats – natural or modified – to ensure the maintenance of biodiversity and the ecological and evolutionary processes. For the last two years and within the outreach programs scenario at Brunca Regional Head Office, Perez Zeledon Campus, we have been working at Alexander Skutch Green Belt. This work has been done through FUNDER (University Funds for Regional Development), focused on the objective of strengthen the efforts the communities that belong to this specific belt do to help in the sustainability of the environment, natural and cultural resources. Because the concept of Green Belt was not well positioned or understood by the communities, they had not a real identification with the meaning of a Protected Green Belt. To develop the research process we started diagnosing each one of the seven communities located on Alexander Skutch Green Belt. The instrument included documentary and bibliographical research, field trips, and open forums to get acquainted with these communities’ economic, social and cultural characteristics and with the leaders of the communities. The most important results gathered during the research at A. S Green Belt, are the identification of the environmental problems in each one of the communities, the local initiatives on protection, get close to the Green Belt Counsel and give support to its members. On the other hand, we showed the people of the communities other significant efforts from communities around the country thus our participation in different activities as festivals, forums and activities in the Green Belt.
Crisis ecológica y determinación social de la salud y la vida
Authors: Sepúlveda Gallego Luz Elena 
Published: September 2022

A partir de una caracterización general de la crisis ecológica que se vive en el planeta, se presenta la multidimensionalidad y complejidad de los conceptos de salud y determinación social de la salud y la Vida, para fundamentar la necesidad de imbricar los enfoques sociológico, económico, ético, político, ecológico ambiental y biológico en la formación de los profesionales en salud que requiere Colombia en el siglo XXI.
Histogram analysis for the differentiation of malignant and benign lesions in breast magnetic resonance imaging: preliminary study
Authors: AĞLAMIŞ Serpil , BAYKARA Murat 
Published: September 2022

Purpose: The present study assesses whether malignant and benign lesions can be distinguished through histogram analysis of non-fat-suppressed T1-weighted and fat-suppressed T2-weighted breast magnetic resonance images (MRIs).
Materials and Methods: MRIs of 20 malignant and 20 benign breast lesions were reviewed retrospectively by histogram analysis performed using Osirix V.4.9 software. The regions of interest (ROIs) were drawn manually to include almost the entire lesion, and values from these ROIs were used to calculate gray-level intensity mean, standard deviation, entropy, uniformity, skewness, kurtosis, and percentile values.
Results: In non-fat-suppressed T1-weighted images, the minimum, 1st, 3rd, 5th, 10th and 25th percentile values were significantly lower in the malignant lesions than in the benign lesions. The minimum value had sensitivity of 70% and specificity of 63.2%. On the fat-suppressed T2-weighted images, skewness was significantly higher while uniformity was significantly lower in malignant lesions than benign lesions. Skewness had 68.4% sensitivity and 60% specificity, and uniformity had 65% sensitivity and 68.4% specificity.
Conclusion: The results of this study demonstrated that histogram analysis of non-fat-suppressed T1-weighted and fat-suppressed T2-weighted images can be used to differentiate malignant and benign lesions in breast MRI.
The effect of CYP2C9 gene polymorphisms on blood pressure lowering response to losartan in patients with essential hypertension
Authors: PEPEDİL TANRİKULU Funda , BABAOGLU Melih O. , ÇAKIR Banu , BOZKURT Atilla , GÜVEN Gülay Sain 
Published: September 2022

Purpose: In this study, the possible effects of CYP2C9 polymorphisms on the clinical response to losartan were investigated in a group of hypertensive patients.
Materials and Methods: Seventy-four patients, newly diagnosed to have essential hypertension, and were subsequently prescribed losartan by attending physicians, were prospectively recruited for the study. Blood pressure measurements at the initiation of losartan treatment and six weeks after were completed for all participants. Genetic analysis for CYP2C9 polymorphisms was performed in blood samples collected at baseline. CYP2C9 *2 and *3 variant alleles were genotyped, and polymorphic patients' treatment responses were compared with the patients' who were carrying the wild type genotype.
Results: Analysis comparing the wild type genotype and CYP2C9*1*2 genotype revealed a trend toward more systolic blood pressure reduction in favor of wild-type genotype. However, there was no statistically significant difference between these two groups considering the change in diastolic blood pressure levels. Regarding the CYP2C9*1*3 genotype, there were no significant differences in systolic or diastolic blood pressure changes.
Conclusion: CYP2C9*1*2 polymorphism affects the systolic blood pressure response to losartan in hypertensive patients, while the CYP2C9*1*3 genotype was not shown in associated with systolic or diastolic blood pressure responses.
Keywords: Hypertension, losartan, CYP2C9, genetic polymorphism, pharmacogenetics
Cinsel istismara uğrayan ergenlerde bağlanma stili, ana-baba tutumu, depresyon ve anksiyetenin değerlendirilmesi
Authors: KELEŞ Şeyma , TOROS Fevziye , GÜLER AKSU Gülen , İNCEL İpek 
Published: September 2022

Amaç: Bu çalışmada cinsel istismar mağduru ergenlerin sosyodemografik özelliklerinin ve uğradıkları cinsel istismarın özelliklerinin tanımlanması; bununla birlikte bu ergenlerin bağlanma stillerinin, ana-baba tutumlarının, depresyon ve anksiyete ölçek puanlarının cinsel istismara uğramayan kontrol grubu ile karşılaştırılması amaçlanmıştır.
Gereç ve Yöntem: Araştırmanın örneklemini 13-18 yaş grubundaki cinsel istismara uğrayan 34 ergen ve cinsel istismara uğramayan 34 ergen oluşturmaktadır. Çalışmaya katılan tüm ergenlerin sosyodemografik verileri toplanmıştır. Cinsel istismara uğrayan ve uğramayan ergenlerin bağlanma stillerini, ebeveyn tutumlarını, depresyon ve anksiyete şiddetini karşılaştırmak için İlişki Ölçekleri Anketi, Ana-baba Tutum Ölçeği, Çocuklar için Depresyon Ölçeği ve Durumluk-Süreklilik Kaygı Envanterinden yararlanılmıştır.
Bulgular: Olguların yaş ortalaması 15,29±1,31 olarak saptanmıştır. Cinsel istismar grubunun korkulu bağlanma alt ölçek puan ortalamaları kontrol grubuna göre anlamlı olarak daha yüksek bulunmuştur. Cinsel istismar grubunun kontrol grubuna göre ebeveyn tutumları açısından otoriter tutum alt ölçek ortalamalarının anlamlı olarak yüksek olduğu görülmüştür. Cinsel istismar grubunun depresyon ve durumluk-süreklilik anksiyete puan ortalamasının kontrol grubuna göre anlamlı olarak yüksek olduğu da saptanmıştır.
Sonuç: Cinsel istismara uğrayan çocuk ve ergenlerin sosyodemografik özelliklerinin, istismara ait özelliklerin ve risk faktörlerinin tanımlanması, anne ve çocuk etkileşiminin arttırılması, güvenli bağlanmanın gerçekleşmesine yönelik ana-baba eğitimlerinin olması, demokratik ana-baba tutumunun benimsenmesi çocuk ve ergenlerin ruh sağlığını korumak açısından son derece önemlidir.
Is neuropathic pain an overlooked symptom in axial spondyloarthritis?
Authors: KAYACAN ERDOĞAN Esra , TÜRK İpek 
Published: September 2022

Purpose: Axial spondyloarthritis (axSpA) is a chronic inflammatory rheumatologic disease. Pain is the most common symptom affecting quality of life in axSpA patients. It has been showed that pain is not always correlated with inflammation in patients. The aim of our study was to investigate the frequency of neuropathic pain in axSpA patients and relationship between neuropathic pain and patient characteristics.
Materials and Methods: Patients diagnosed as axSpA according to the Assessment of SpondyloArthritis International Society classification criteria, who presented in our outpatients clinics from January to March 2019 were included in this study. The neuropathic component of pain was evaluated with Douleur Neuropathique en 4 Questions (DN4) questionnaire.
Results: Eighty seven axSpA patients were included in the study. Thirty of patients had neuropathic pain according to DN4 questionnaire (DN4>4). Neuropathic pain was higher in active disease group depending on both of ASDAS-CRP and BASDAI. DN4 score of patients was found moderately correlated with ASDAS-CRP and BASDAI score. A weak positive correlation was found between patients education level and DN4 score.
Conclusion: We showed that neuropathic pain could be seen in patients with axSpA and it could be correlated with disease activity. The studies have proven that neuropathic pain could lead to impaired quality of life and social & emotional functions. Patients with neuropathic pain could not benefit from antiinflammatory treatments. Therefore evaluating of neuropathic pain is important in axSpA patients. Further studies on management of neuropathic pain in axSpA patients are needed.
Multipl sklerozda spinal kord lezyonları ile kognisyon ilişkisi
Authors: DİKER Sevda , EKER Amber , KAYMAKAMZADE Doç Dr Bahar , EREM Aysegul , BALYEMEZ Uğurcan 
Published: September 2022

Amaç: Çalışmamızda, multipl skleroz (MS)da fiziksel özürlülük ve prognozla yakın ilişkisi olan spinal kord tutulumunun, kognitif işlevler üzerine olası etkisi incelenmiştir.
Gereç ve Yöntem: Çalışmaya 29 MS hastası (2 klinik izole sendrom, 23 relaps ve remisyonlarla seyreden MS, 2 sekonder progresif MS, 2 primer progresif MS) alındı. Demografik özellikleri, genişletilmiş özürlülük durum skalası (EDSS) skoru, Rao’nun kısa tekrarlanabilir nöropsikolojik test bataryası ile değerlendirilen kognitif durumu, beyin ve spinal kord T2 lezyon sayısı değerlendirildi. Benzer demografik özelliklere sahip 17 sağlıklı kontrol ile kognitif test sonuçları karşılaştırıldı.
Bulgular: İleri yaş, hastalık başlangıç yaşının ileri olması, kısa eğitim süresi kognitif test sonuçlarını olumsuz etkileyen özelliklerdir. Beyin ve spinal kord lezyon sayılarının kognitif testler üzerine anlamlı etkisi saptanmamıştır. Spinal kord lezyonu olan hastalar olmayanlara göre daha yüksek EDSS’ye sahiptir, ancak iki grup arasında kognitif test sonuçları açısından anlamlı farklılık saptanmamıştır.
Sonuç: Spinal kord lezyonları, EDSS ile değerlendirilen fiziksel özürlülük ile ilişkilidir. Kord plak varlığının ya da sayısının kognitif test sonuçları üzerine etkisini, servikal kord atrofisi ile birlikte değerlendirecek, daha geniş örneklemli boylamsal çalışmalara ihtiyaç vardır.
The Role of Christian Education in Building Skills and Instilling Moral Values among Students in Higher Ed Institutions for National Unity and Economic Advancement in Sub-Saharan Africa
Authors: Chukwuma Victor Priest 
Published: September 2022

Education is more than the acquisition of knowledge. There are people in society who have acquired expertise but lack the character and skills to impact their community effectively. This situation has distorted national unity and lowered economic growth. “African graduates, especially in Sub-Saharan Africa, are victims of frequent ethnic conflicts, corruption, nepotism and industries dependent on outdated technology with low returns and low productivity” (Kigotho 2019). Many students graduate from higher ed institutions to cause unrest in their country because of a lack of skills and morals. To revolutionize the education sector in Sub-Saharan Africa requires an integration of biblical principles in the teaching and learning process to build skills and character for national advancement. The approach used in this paper is library research through critical analysis of empirical studies and library resources, both primary and secondary sources, to describe the phenomenon under study
Financial Literacy: A Bibliometric Analysis for the Period of 1990-2021
Authors: Alsmadi Ayman Abdalmajeed 
Published: September 2022

Financial literacy can be defined as the ability to understand and effectively use various financial skills, including personal financial management, budgeting, and investing. Financial literacy is the foundation of your relationship with money, and it is a lifelong journey of learning. This paper analyses the publications on Financial literacy, and their intellectual structure and networking. The bibliometric data on Financial literacy research have been extracted from the Scopus database. This study finds the most productive countries, universities, authors, journals, and most prolific publications in Financial literacy, by examining the published works. Also, the study visualizes the intellectual network by mapping bibliographic coupling (BC) and co-citation. The study’s essential contribution is the analysis of Financial literacy developments and trajectories that can help scholars and practitioners to appreciate the trend and future studies.

Keywords: Financial literacy; systematic review; future research; bibliometric analysis.
Penentuan Strategi Promosi UMKM Fashion dan Souvenir dengan Metode Market Basket Analysis
Authors: Wiliyanto Reza , Mahirah Alifia , Firmansyah Faisal Ibnu 
Published: September 2022

Abstrak
Sebagian besar pelaku usaha mikro, kecil dan menengah (UMKM) di Indonesia mengalami tren penurunan penjualan selama pandemi Covid-19. Berdasarkan survei yang dilakukan oleh Asosiasi Business Development Service Indonesia (ABDSI) terhadap 6.000 UMKM yang tersebar diseluruh Indonesia dengan periode April-Mei 2020, sebanyak 92,5% UMKM mengalami penurunan penjualan 60-100%. Tujuan penelitian ini adalah mengupayakan peningkatan penjualan melalui strategi promosi bundling yang sesuai dengan perilaku pembelian konsumen. Metode pendekatan yang digunakan adalah Market Basket Analysis. Adapun tahapannya terdiri dari Data Collection, Pre-Processing, Transformation dan Interpretation. Studi kasus penelitian ini adalah salah satu UMKM yang bergerak di bidang fashion dan souvenir di Kota Serang. Data yang digunakan adalah data penjualan dari tahun 2020 – 2021. Hasil penelitian didapatkan bahwa algoritma apriori dapat digunakan untuk strategi bundling pada toko helianthe(dot)project. Dengan nilai confidence > 0,9, nilai support > 0,9, dan nilai lift ratio > 1 diperoleh strategi bundling yang terpilih, yaitu 3 kombinasi yang berisi 2 item produk dan 1 kombinasi yang berisi 3 item produk.