bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated plasma and urinary erythritol is a biomarker of excess simple carbohydrate intake in mice
Authors: Ortiz Semira R. , Field Martha S. 
Published: December 2022

AbstractBackgroundElevated serum erythritol is a predictive biomarker of diabetes and cardiovascular incidence and complications. Erythritol is synthesized endogenously from glucose, but little is known regarding the origin of elevated circulating erythritolin vivo.ObjectiveIn vitroevidence indicates that intracellular erythritol is elevated by high-glucose cell culture conditions and that final step of erythritol synthesis is catalyzed by the enzymes SORD and ADH1. The purpose of this study was to determine if dietary intake and/or diet-induced obesity (DIO) affect erythritol synthesis in mice, and if this relationship is modified by loss of the enzymes SORD or ADH1.MethodsFirst, 8-week-old, maleSord+/+,Sord-/-,Adh1+/+, andAdh1-/-mice were fed either low-fat diet (LFD) with 10% fat-derived calories or DIO high-fat diet (HFD) with 60% fat-derived calories for 8 weeks. Plasma and tissue erythritol were measured using GC-MS. Second, wild-type 8-week-old C57BL/6J mice were fed LFD or HFD with plain drinking water or 30% sucrose water for 8 weeks. Blood glucose and plasma and urinary erythritol were measured in non-fasted and fasted samples. Tissue erythritol was measured following sacrifice. Finally,Sord+/+andSord-/-mice were fed LFD with 30% sucrose water for two weeks, then non-fasted plasma, urine, and tissue erythritol were quantified.ResultsPlasma and tissue erythritol were not impacted by loss ofSordorAdh1on LFD or HFD. In wild-type mice, consumption of 30% sucrose water significantly elevated plasma and urinary erythritol on both LFD and HFD compared to plain water.Sordgenotype did not affect plasma or urinary erythritol in response to sucrose feeding, butSord-/-mice had reduced kidney erythritol content compared to wildtype littermates in response to sucrose.ConclusionsSucrose intake, not high-fat diet, elevates erythritol synthesis and excretion in mice. Loss of ADH1 or SORD does not significantly impact erythritol levels in mice.
A novel type of monocytic leukemia stem cell revealed by the clinical use of venetoclax-based therapy
Authors: Pei Shanshan , Gillen Austin E , Shelton Ian T , Stevens Brett M , Gasparetto Maura , Engel Krysta , Staggs Sarah , Wang Yanan , Showers William , Inguva Anagha , Amaya Maria L , Minhajuddin Mohammad , Winters Amanda , Patel Sweta B , Tolison Hunter , Krug Anna , Young Tracy N , Schowinsky Jeffrey , McMahon Christine , Smith Clayton A , Pollyea Daniel A , Jordan Craig T 
Published: December 2022

AbstractThe BCL-2 inhibitor venetoclax has recently emerged as an important component of acute myeloid leukemia (AML) therapy. Notably, use of this agent has revealed a previously unrecognized form of pathogenesis characterized by monocytic disease progression. We demonstrate that this form of disease arises from a fundamentally different type of leukemia stem cell (LSC), which we designate as monocytic LSC (m-LSC), that is developmentally and clinically distinct from the more well-described primitive LSC (p-LSC). The m-LSC is distinguished by a unique immunophenotype (CD34-, CD4+, CD11b-, CD14-, CD36-), unique transcriptional state, reliance on purine/pyrimidine metabolism, and selective sensitivity to cladribine. Critically, in some instances m-LSC and p-LSC subtypes can co-reside in the same AML patient and simultaneously contribute to overall tumor complexity. Thus, our findings demonstrate that LSC heterogeneity has direct clinical significance and highlights the need to distinguish and target m-LSCs as a means to improve clinical outcomes with venetoclax-based regimens.Statement of SignificanceThese studies identify and characterize a new type of human acute myeloid leukemia stem cell (LSC) that is responsible for monocytic disease progression in acute myeloid leukemia (AML) patients treated with venetoclax-based regimens. Our studies describe the phenotype, molecular properties, and drug sensitivities of this unique LSC subclass.
Coordinated Regulation of Cdc42ep1, Actin, and Septin Filaments during Neural Crest Cell Migration
Authors: Kho Mary , Hladyshau Siarhei , Tsygankov Denis , Nie Shuyi 
Published: December 2022

ABSTRACTThe septin cytoskeleton has been demonstrated to interact with other cytoskeletal components to regulate various cellular processes, including cell migration. However, the mechanisms of how septin regulates cell migration are not fully understood. In this study, we use the highly migratory neural crest cells of frog embryos to examine the role of septin filaments in cell migration. We found that septin filaments are required for proper migration of neural crest cells by controlling both the speed and the direction of cell migration. We further determined that septin filaments regulate these features of cell migration by interacting with actin stress fibers. In neural crest cells, septin filaments co-align with actin stress fibers, and the loss of septin filaments leads to impaired stability and contractility of actin stress fibers. In addition, we showed that a partial loss of septin filaments leads to drastic changes in the orientations of newly formed actin stress fibers, suggesting that septin filaments help maintain the persistent orientation of actin stress fibers during directed cell migration. Lastly, our study revealed that these activities of septin filaments depend on Cdc42ep1, which co-localizes with septin filaments in the center of neural crest cells. Cdc42ep1 interacts with septin filaments in a reciprocal manner, with septin filaments recruiting Cdc42ep1 to the cell center and Cdc42ep1 supporting the formation of septin filaments.
A circuit from the locus coeruleus to the anterior cingulate cortex modulates offspring interactions in mice
Authors: Corona Alberto , Choe Jane , Muñoz-Castañeda Rodrigo , Osten Pavel , Shea Stephen D. 
Published: December 2022

SUMMARYSocial sensitivity to other individuals in distress is crucial for survival. The anterior cingulate cortex (ACC) is a structure involved in making behavioral choices and is influenced by observed pain or distress. Nevertheless, our understanding of the neural circuitry underlying this sensitivity is incomplete. Here, we reveal unexpected sex-dependent activation of ACC when parental mice respond to distressed pups by returning them to the nest (‘pup retrieval’). We observe sex differences in the interactions between excitatory and inhibitory ACC neurons during parental care, and inactivation of ACC excitatory neurons increased pup neglect. Locus coeruleus (LC) releases noradrenaline in ACC during pup retrieval, and inactivation of the LC-ACC pathway disrupts parental care. We conclude that ACC maintains sex-dependent sensitivity to pup distress under LC modulation. We propose that ACC’s involvement in parenting presents an opportunity to identify neural circuits that support sensitivity to the emotional distress of others.
The exception that proves the rule: Virulence gene expression at the onset ofPlasmodium falciparumblood stage infections
Authors: Wichers-Misterek Jan Stephan , Krumkamp Ralf , Held Jana , Thien Heidrun von , Wittmann Irene , Höppner Yannick Daniel , Ruge Julia M. , Moser Kara , Dara Antoine , Strauss Jan , Esen Meral , Fendel Rolf , Sulyok Zita , Kremsner Peter G. , Sim B. Kim Lee , Hoffman Stephen L. , Duffy Michael F. , Otto Thomas D. , Gilberger Tim-Wolf , Silva Joana C. , Mordmüller Benjamin , Petter Michaela , Bachmann Anna 
Published: December 2022

AbstractControlled human malaria infections (CHMI) are a valuable tool to study parasite gene expressionin vivounder defined conditions. In previous studies, virulence gene expression was analyzed in samples from volunteers infected with thePlasmodium falciparum(Pf) NF54 isolate, which is of African origin. Here, we provide an in-depth investigation of parasite virulence gene expression in malaria-naïve European volunteers undergoing CHMI with the genetically distinct Pf 7G8 clone, originating in Brazil. Differential expression ofvargenes, encoding major virulence factors of Pf, PfEMP1s, was assessed inex vivoparasite samples as well as in parasites from thein vitrocell bank culture that was used to generate the sporozoites (SPZ) for CHMI (Sanaria® PfSPZ Challenge (7G8)). We report broad activation of mainly B-type subtelomeric locatedvargenes at the onset of a 7G8 blood stage infection in naïve volunteers, mirroring the NF54 expression study and suggesting that the expression of virulence-associated genes is generally reset during transmission from the mosquito to the human host. However, in 7G8 parasites, we additionally detected a continuously expressed single C-type variant, Pf7G8_040025600, that was most highly expressed in both pre-mosquito cell bank and volunteer samples, suggesting that 7G8, unlike NF54, maintains expression of some previously expressedvarvariants during transmission. This suggests that in a new host, the parasite may preferentially express the variants that previously allowed successful infection and transmission.
Angels, demons and empowering leadership: simultaneous compensatory links to work role performance
Authors: Martínez-Córcoles Mario , Peñarroja Vicente , Stephanou Konstantinos 
Published: December 2022

PurposePrior research indicates that empowering leadership has simultaneous contradictory effects on work performance. This study aimed to explore contradictory mechanisms through which empowering leadership is related to work role performance behaviors.Design/methodology/approachThe sample was composed of 274 professionals from five IT companies located in the Baltic area. OLS regression analyses were performed using MEDCURVE for SPSS 23.0.FindingsEmpowering leadership is positively related to work role performance behaviors; additionally, perceived uncertainty mediates the relationship between empowering leadership and work role performance behaviors, with the relationship between empowering leadership and uncertainty having a curvilinear U-shape (concave upward). That is, although empowering leadership is positively related to work role performance, the relationship between empowering leadership and work role performance though uncertainty becomes non-significant at high levels of empowering leadership.Originality/valueThis is one of the first studies to demonstrate that empowering leadership is related to work performance through simultaneous compensatory mechanisms. Moreover, this study provides evidence about the curvilinear relationship between empowering leadership and performance through uncertainty (previously unknown).
Employees' reactions to CSR perception and disclosure in the presence of multilevel contingencies
Authors: Mahmood Faisal , Saleem Maria , Qadeer Faisal , Ariza-Montes Antonio , Han Heesup 
Published: December 2022

PurposePrimarily, this research aims to examine how and when firm-level corporate social responsibility (CSR) translates into individual-level attitudes and behaviors of employees under cross-level boundary conditions of firm-level family ownership (FO) and group-level ethical leadership.Design/methodology/approachPhilosophically, the present research comes under the post-positivist paradigm, with a deductive approach. The multilevel, multisource and multimethod data for this research were collected by employing a time-lagged design through the survey strategy and from annual reports of 60 manufacturing firms in Pakistan. The multilevel path analysis was conducted using MPlus.FindingsThe authors found that organizational identification (OID) statistically and significantly mediates the impact of firms' CSR disclosure on employees' innovative job performance (EIJP). However, the partial mediation of OID between firm-level CSR perception and EIJP was noticed. Moreover, a firm-level contingency of FO and group-level ethical leadership further intensifies the impact of CSR disclosure and perception on EIJP through OID.Research limitations/implicationsTheoretically, this research widens the current understanding of employees' reactions to firms' CSR disclosure and perception by investigating the contingencies of firm-level FO and group-level ethical leadership. Practically, the managers can consider the underlying framework presented in this research in defining CSR as the antecedent of the OID and EIJP. For example, organizations must deliberately concentrate on not only their CSR initiatives and engagements but also immense attentiveness should be given to CSR disclosure because disclosing CSR will assist the top management in achieving the desired workplace attitudes and behaviors of employees. This research will also help business leaders to understand the integration of CSR and ethical leadership while making CSR-related strategic decisions.Originality/valueExisting research on CSR still needs advancement due to competing explanations, inconsistencies in the findings, and a lack of multilevel studies. Although few studies on CSR have considered multilevel aspects by devising and testing multilevel mechanisms but largely remained deficient concerning cross-level boundary conditions. Furthermore, the authors also noticed that the academic literature predominantly analyses the impact of perceived CSR either at the individual level or the firm aggregated level on employee attitudes and behaviors. However, research on the effect of organizational CSR disclosure on the behaviors and attitudes of employees remains scarce.
Asset management-based resilience index formulation for pavements via principal components analysis
Authors: Mohammed Ahmed , Zayed Tarek , Nasiri Fuzhan , Bagchi Ashutosh 
Published: December 2022

Purpose
This paper extends the authors’ previous research work investigating resilience for municipal infrastructure from an asset management perspective. Therefore, this paper aims to formulate a pavement resilience index while incorporating asset management and the associated resilience indicators from the authors’ previous research work.

Design/methodology/approach
This paper introduces a set of holistic-based key indicators that reflect municipal infrastructure resiliency. Thenceforth, the indicators were integrated using the weighted sum mean method to form the proposed resilience index. Resilience indicators weights were determined using principal components analysis (PCA) via IBM SPSS®. The developed framework for the PCA was built based on an optimization model output to generate the required weights for the desired resilience index. The output optimization data were adjusted using the standardization method before performing PCA.

Findings
This paper offers a mathematical approach to generating a resilience index for municipal infrastructure. The statistical tests conducted throughout the study showed a high significance level. Therefore, using PCA was proper for the resilience indicators data. The proposed framework is beneficial for asset management experts, where introducing the proposed index will provide ease of use to decision-makers regarding pavement network maintenance planning.

Research limitations/implications
The resilience indicators used need to be updated beyond what is mentioned in this paper to include asset redundancy and structural asset capacity. Using clustering as a validation tool is an excellent opportunity for other researchers to examine the resilience index for each pavement corridor individually pertaining to the resulting clusters.

Originality/value
This paper provides a unique example of integrating resilience and asset management concepts and serves as a vital step toward a comprehensive integration approach between the two concepts. The used PCA framework offers dynamic resilience indicators weights and, therefore, a dynamic resilience index. Resiliency is a dynamic feature for infrastructure systems. It differs during their life cycle with the change in maintenance and rehabilitation plans, systems retrofit and the occurring disruptive events throughout their life cycle. Therefore, the PCA technique was the preferred method used where it is data-based oriented and eliminates the subjectivity while driving indicators weights.
How does differential leadership affect team decision-making effectiveness? The role of thriving at work and cooperative goal perception
Authors: Niu Lixia , Zhao Rui , Wei Yisong 
Published: December 2022

Purpose
The purpose of this paper is to explore the mechanism and boundary conditions in the relationship between differential leadership and team decision-making effectiveness.

Design/methodology/approach
A total of 366 valid questionnaires were received from employees of high-tech enterprises in China, regression-based moderation and bootstrapping analyses were adapted to analyze data and test hypotheses by using the PROCESS syntax in SPSS software.

Findings
Differential leadership can positively contribute to team decision-making effectiveness, and thriving at work mediates the relationship between the two, and cooperative goal perception plays a moderating role in the relationship between thriving at work and team decision-making effectiveness and cooperative goal perception moderate the mediating effect of thriving at work between differential leadership and team decision-making effectiveness.

Practical implications
The findings suggest that managers need to focus on leadership style to promote improved team decision-making effectiveness by enhancing thriving at work and cooperative goal perception.

Originality/value
Overall, this study is based on the conservation of resources theory to uncover the “black box” between differential leadership and team decision-making effectiveness and to highlight the important role of thriving at work and cooperative goal perception.
Nascent entrepreneurship: a critical perspective and research agenda in tourism and hospitality
Authors: Dias Álvaro , González-Rodríguez M. Rosario , Hallak Rob 
Published: December 2022

Purpose
This study aims to systematize the drivers of nascent entrepreneurship in tourism and to suggest avenues for future research. As a consequence of the pandemic, a reduction in early-stage entrepreneurial activity was reported worldwide. The countries that responded best to this situation were those that fostered entrepreneurship at this early stage, designated as nascent. Hence, research on nascent entrepreneurs requires particular attention.

Design/methodology/approach
To address this study’s goal, the authors have examined the development of nascent entrepreneur literature in the last two decades and discussed how the literature on tourism nascent entrepreneurship relates to the mainstream literature in terms of theoretical frameworks. The authors explored specificities of the tourism industry to propose new research avenues to explore the theme of new venture creation in the hospitality and tourism sector.

Findings
The authors divided the implication of tourism specificities into main themes: motivations, human and social capital and government and incubators. Several research questions for future research are proposed.

Practical implications
By focusing on nascent entrepreneurship, researchers and policymakers can obtain important insights from projects that have not been implemented, going beyond those that have been successfully undertaken, as aimed at in entrepreneurship research.

Originality/value
This research contributes to the nascent tourism entrepreneurship literature by providing theoretical and empirical research questions to advance existing knowledge in tourism nascent entrepreneurship.
Remittances and renewable energy: an empirical analysis
Authors: Subramaniam Yogeeswari , Masron Tajul Ariffin , Loganathan Nanthakumar 
Published: December 2022

PurposeThe purpose of this paper is to examine the potential role of remittances on renewable energy consumption in the top recipient developing countries from 1990 to 2016.Design/methodology/approachThe paper uses autoregressive distributed lag (ARDL) technique to fulfil the purpose.FindingsThe empirical findings divulge that remittances positively affect renewable energy consumption. This finding implies that remittances can potentially increase the level of renewable energy consumption by increasing affordability if proper incentives and encouragement are offered.Practical implicationsGiven the enormous potential that renewable energy can bring to an economy, the government should offer indirect incentives to encourage recipients to allocate a portion of their remittances to renewable energy projects, either as minor investors or users.Originality/valueTo the best of the authors’ knowledge, this paper is novel for two reasons. First, this study adds to the existing literature by empirically examining the link between remittances and renewable energy consumption in the top five remittance recipients, which have never been studied before. Second, the findings of this study will have policy implications not only for the top remittance recipients but also for other remittance recipients, particularly for developing countries.
Environmental performance of servitized manufacturing firms: the (mis)alignment between servitization strategies and inter-organizational information technology capabilities
Authors: Zhang Minghua , Chen Meng , Zhang Mengru , Liu Hefu 
Published: December 2022

PurposeThis study investigates the different effects of the (mis)alignment between servitization strategies (i.e. product-oriented and customer-oriented services) and inter-organizational information technology (IT) capabilities (i.e. IT reconfiguration and integration) concerning the environmental performance of servitized manufacturing firms.Design/methodology/approachUsing hierarchical regression analysis, this study examines the theoretical model based on multi-respondent survey data from 1,149 senior managers of 383 manufacturers in China.FindingsProduct-oriented services have a non-significant effect on environmental performance, whereas customer-oriented services exert a significantly positive effect. The alignment between product-oriented services and IT reconfiguration, and that between customer-oriented services and IT integration, improve environmental performance. In contrast, the misalignment between product-oriented services and IT integration, and that between customer-oriented services and IT reconfiguration, hurt environmental performance.Originality/valueThis study elucidates the different effects of the (mis)alignment between servitization strategies and inter-organizational IT capabilities on environmental performance. It also resolves the inconsistency regarding the implications of servitization on environmental performance.