bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dyadic versus individual delivery of a yoga program for family caregivers of glioma patients undergoing radiotherapy: Results of a 3‐arm randomized controlled trial
Authors: Milbury Kathrin , Whisenant Meagan , Weathers Shiao‐Pei , Malliaha Smitha , Snyder Stella , Jackson Natalie , Li Jing , Li Yisheng , Silva Roseanglea F. , Shih Ya‐Chen Tina , Cohen Lorenzo 
Published: December 2022

AbstractBackgroundDespite their significant distress, supportive care interventions for caregivers of glioma patients are generally lacking. And, whether caregivers are more likely to benefit from interventions targeting patient‐caregiver dyads or caregivers individually is unknown. This pilot randomized controlled trial compared the feasibility and preliminary efficacy of a dyadic yoga (DY) versus an individual caregiver yoga (CY) intervention as a supportive care strategy for family caregivers.MethodsPatient‐caregiver dyads were randomized to a DY, CY or usual care (UC) arm. DY and CY interventions were delivered over 15 sessions. Caregivers completed assessments of their depressive symptoms, quality of life (QOL), and caregiving reactions at baseline, 6 weeks, and 12 weeks, and a subset completed qualitative interviews at 12 weeks.ResultsWith a consent rate of 63%, 67 dyads were randomized. Attendance in the DY was higher than in the CY group (session means, DY = 12.23, CY = 9.00; p = 0.06). Caregivers (79% female; 78% non‐Hispanic White; mean age, 53 years) reported significantly more subjective benefit in the CY arm than in the DY arm (d = 2.1; p < .01), which was consistent with the qualitative assessment. There were medium effect sizes for improved mental QOL (d = 0.46) and financial burden (d = 0.53) in favor of the CY over the UC group. Caregivers in the CY group reported more caregiving esteem (d = 0.56) and less health decline (d = 0.60) than those in the DY group.ConclusionIndividual rather than dyadic delivery may be a superior supportive care approach for this vulnerable caregiver population. A larger, adequately powered efficacy trial is warranted.
Genotyping Microbial Communities with MIDAS2: From Metagenomic Reads to Allele Tables
Authors: Zhao Chunyu , Goldman Miriam , Smith Byron J. , Pollard Katherine S. 
Published: December 2022

AbstractThe Metagenomic Intra‐Species Diversity Analysis System 2 (MIDAS2) is a scalable pipeline that identifies single nucleotide variants and gene copy number variants in metagenomes using comprehensive reference databases built from public microbial genome collections (metagenotyping). MIDAS2 is the first metagenotyping tool with functionality to control metagenomic read mapping filters and to customize the reference database to the microbial community, features that improve the precision and recall of detected variants. In this article we present four basic protocols for the most common use cases of MIDAS2, along with supporting protocols for installation and use. In addition, we provide in‐depth guidance on adjusting command line parameters, editing the reference database, optimizing hardware utilization, and understanding the metagenotyping results. All the steps of metagenotyping, from raw sequencing reads to population genetic analysis, are demonstrated with example data in two downloadable sequencing libraries of single‐end metagenomic reads representing a mixture of multiple bacterial species. This set of protocols empowers users to accurately genotype hundreds of species in thousands of samples, providing rich genetic data for studying the evolution and strain‐level ecology of microbial communities. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Species prescreeningBasic Protocol 2: Download MIDAS reference databaseBasic Protocol 3: Population single nucleotide variant callingBasic Protocol 4: Pan‐genome copy number variant callingSupport Protocol 1: Installing MIDAS2Support Protocol 2: Command line inputsSupport Protocol 3: Metagenotyping with a custom collection of genomesSupport Protocol 4: Metagenotyping with advanced parameters
Growth of Zn‐Ge‐O Thin‐Film as a Transparent Conductive Oxide Buffer Material for Chalcopyrite Solar Cell
Authors: Egyna Dwinanri , Ito Satoru , Nishimura Takahito , Yamada Akira 
Published: December 2022

AbstractThe importance of positive band offset at the p–n interface of heterojunction thin‐film solar cells has been underlined in the authors' previous work on theoretical study of the role of n‐type layer in determining open‐circuit voltage – and experimental work by other research groups. An n‐type material with low electron affinity has the potential to fulfill the requirements for producing a high‐performance heterojunction thin‐film solar cell. In this current work, a new alternative transparent conductive oxide material for chalcopyrite heterojunction solar cells is investigated by alloying ZnO and GeO2 to grow a Zn‐Ge‐O thin‐film with low electron affinity. The film is grown by metal organic chemical vapor deposition with tetramethoxygermanium as the Ge source. Through film characterization, it is confirmed that higher concentrations of Ge in the film result in lower electron affinities and relatively constant valence band maximums. Different [Ge] / ([Zn] + [Ge]) also affects the film morphology, from polycrystalline at a lower concentration of Ge to aggregation of nanocrystals at a higher concentration of Ge. Electronic characterization and preliminary device application test show promising results for the future of the Zn‐Ge‐O thin‐film as an alternative n‐type layer for chalcopyrite solar cells.
Agronomic Practices to Minimize Lodging While Maximizing Yield and Protein of Spring Wheat in the Eastern Canadian Prairies
Authors: Mangin Amy , Lawley Yvonne 
Published: December 2022

AbstractNew high‐yielding spring wheat cultivars are being grown widely across the Canadian Prairies. As yields rise, grain protein levels can only be maintained by increasing the N availability to crop, often reducing fertilizer use efficiency. An additional concern when increasing the amount of N fertilizer being applied to the crop is the increased risk of lodging, reducing the crop’s yield, quality, and harvestability. This article reports on research evaluating the effect of agronomic management practices on spring wheat lodging risk, grain yield, and protein content in the eastern Canadian Prairies. Earn 0.5 CEUs in Crop Management by reading this article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.
A basic community dynamics experiment: Disentangling deterministic and stochastic processes in structuring ecological communities
Authors: Jewell Mark Davidson , Bell Graham 
Published: December 2022

AbstractCommunity dynamics are governed by two opposed processes: species sorting, which produces deterministic dynamics leading to an equilibrium state, and ecological drift, which produces stochastic dynamics. Despite a great deal of theoretical and empirical work aiming to demonstrate the predominance of one or the other of these processes, the importance of drift in structuring communities and maintaining species diversity remains contested. Here, we present the results of a basic community dynamics experiment using floating aquatic plants, designed to measure the relative contributions of species sorting and ecological drift to community change over about a dozen generations. We found that species sorting became overwhelmingly dominant as the experiment progressed, and directed communities toward a stable equilibrium state maintained by negative frequency‐dependent selection. The dynamics of any particular species depended on how far its initial frequency was from its equilibrium frequency, however, and consequently the balance of sorting and drift varied among species.
Microclimate, soil chemistry, and microbiota fail to explain Euphorbia dendroidesJanzen‐Connell pattern in a shrubland
Authors: Idbella Mohamed , Abd‐ElGawad Ahmed M. , Mazzoleni Stefano , Bonanomi Giuliano 
Published: December 2022

AbstractNegative plant–soil feedback can cause density and distance effects that lead to Janzen–Connell (JC) distribution. The JC hypothesis has been proposed to explain the high species diversity in tropical forests, but it has been rarely tested in species‐rich Mediterranean shrublands. In this study, we assessed whether the JC hypothesis could explain Euphorbia dendroides recruitment pattern under conspecific conditions, that is, mother plant and the canopy of Pistacia lentiscus, Juniperus phoenicea, Myrtus communis, Rosmarinus officinalis, and Olea europaea. We examined whether soil chemistry and/or soil microbiota, characterized by both bacterial and fungal communities, explained the observed JC pattern. We also explored differences in microclimate among shrub species by monitoring air temperature and light availability at different times of the year. Euphorbia recruits were almost absent under the canopy of conspecifics, being more abundant at the edge of the canopy, which fits the JC pattern. Interestingly, we also found a high density of Euphorbia recruitment under heterospecific canopies, that is, O. europaea and J. phoenicea, but a very low density under other heterospecific canopies, for example, R. officinalis and P. lentiscus. Although shrubs have a species‐specific microbiota signature under their canopy, neither the accumulation of species‐specific pathogens nor soil chemical properties explain the observed JC pattern. Despite finding that shrubs buffer high temperatures in summer, the observed microclimatic difference failed to explain the recruitment pattern. We conclude that JC is prevalent in this species‐rich Mediterranean shrubland, but the underlying mechanism remains to be investigated and clarified.
Relation of amino acid composition, hydrophobicity, and molecular weight with antidiabetic, antihypertensive, and antioxidant properties of mixtures of corn gluten and soy protein hydrolysates
Authors: Mirzaee Homaira , Ahmadi Gavlighi Hassan , Nikoo Mehdi , Udenigwe Chibuike C. , Khodaiyan Faramarz 
Published: December 2022

AbstractNew mixed Alcalase‐hydrolysates were developed using corn gluten meal (CP) and soy protein (SP) hydrolysates, namely CPH, SPH, SPH30:CPH70, SPH70:CPH30, and SPH50:CPH50. Amino acid profile, surface hydrophobicity (H0), molecular weight (MW) distribution, antioxidant activity, angiotensin‐converting enzyme (ACE), α‐amylase, and α‐glucosidase inhibitory activities, and functional characteristics of hydrolysates were determined. Hydrolysis changed the amount of hydrophilic and hydrophobic amino acid composition and significantly increased the H0 values of hydrolysates, especially for CPH. The DPPH radical scavenging activity (RSA) was higher for CPH, SPH30:CPH70, and SPH50:CPH50 than SPH and SPH70:CPH30. Moreover, SPH, SPH70:CPH30, and SPH50:CPH50 showed lower MW than CPH, and this correlated with the higher hydrophilicity, and ABTS and hydroxyl RSA values obtained for SPH and the mixed hydrolysates with predominantly SPH. SPH70:CPH30 exhibited higher ACE, α‐glucosidase, and α‐amylase inhibitory activities among all samples due to its specific peptides with high capacity to interact with amino acid residues located at the enzyme active site and also low binding energy. At 15% degree of hydrolysis, both SPH and CPH showed enhanced solubility at pH 4.0, 7.0 and 9.0, emulsifying activity, and foaming capacity. Taken together, SPH70:CPH30 displayed strong antioxidant, antihypertensive, and antidiabetic attributes, emulsifying activity and stability indexes, and foaming capacity and foaming stability, making it a promising multifunctional ingredient for the development of functional food products.
Properties of NbC/а‐C:H films on titanium bipolar plates for proton exchange membrane fuel cells
Authors: Gou Yong , Jiang Guang , Geng Jiangtao , Shao Zhigang 
Published: December 2022

AbstractSurface modification of metallic bipolar plates is a crucial subject for the performance elevation of proton exchange membrane fuel cells (PEMFCs). In this work, a series of NbC/а‐C:H films with different Nb/C ratios are prepared by arc ion plating. Film microstructure, composition, mechanical properties, hydrophobility, interfacial contact resistance (ICR), and corrosion resistance in the simulated cathode environment of PEMFCs are systematically studied. The results show that within the experiment conditions, higher NbC content helps to promote the film hardness and adhesion strength as well as the interfacial conductivity. While higher а‐C:H content attributes to a more compact microstructure thus improving the anti‐corrosion performance. The best corrosion resistance and conductivity come with the lowest corrosion current density of 0.09 µA/cm2 and ICR of 0.77 mΩ cm2, respectively. Based on the result of this research, to further improve the comprehensive performance of NbC/а‐C:H film, strategies for increasing the metal carbide content and preventing surface metal oxidation while keeping a dense and fine microstructure need to be considered.
Temporal changes in the microglial proteome of male and female mice after a diffuse brain injury using label‐free quantitative proteomics
Authors: Doust Yasmine V. , Bindoff Aidan , Holloway Olivia G. , Wilson Richard , King Anna E. , Ziebell Jenna M. 
Published: December 2022

AbstractTraumatic brain injury (TBI) triggers neuroinflammatory cascades mediated by microglia, which promotes tissue repair in the short‐term. These cascades may exacerbate TBI‐induced tissue damage and symptoms in the months to years post‐injury. However, the progression of the microglial function across time post‐injury and whether this differs between biological sexes is not well understood. In this study, we examined the microglial proteome at 3‐, 7‐, or 28‐days after a midline fluid percussion injury (mFPI) in male and female mice using label‐free quantitative proteomics. Data are available via ProteomeXchange with identifier PXD033628. We identified a reduction in microglial proteins involved with clearance of neuronal debris via phagocytosis at 3‐ and 7‐days post‐injury. At 28 days post‐injury, pro‐inflammatory proteins were decreased and anti‐inflammatory proteins were increased in microglia. These results indicate a reduction in microglial clearance of neuronal debris in the days post‐injury with a shift to anti‐inflammatory function by 28 days following TBI. The changes in the microglial proteome that occurred across time post‐injury did not differ between biological sexes. However, we did identify an increase in microglial proteins related to pro‐inflammation and phagocytosis as well as insulin and estrogen signaling in males compared with female mice that occurred with or without a brain injury. Although the microglial response was similar between males and females up to 28 days following TBI, biological sex differences in the microglial proteome, regardless of TBI, has implications for the efficacy of treatment strategies targeting the microglial response post‐injury.
Withdrawn: Investigating the Role of Fuel Injection Pressure and Piston Bowl Geometries to Enhance Performance and Emission Characteristics of Hydrogen‐Enriched Diesel/1‐Pentanol Fuelled in CRDI Diesel Engine
Authors: Seelam Navyasree , Gugulothu S.K , Bhasker Burra , panda Jibitesh Kumar , Reddy Ragireddy Venkat 
Published: December 2022

The above article from Heat Transfer, published online on 5 December 2022, on Wiley Online Library (https://onlinelibrary.wiley.com/doi/abs/10.1002/htj.22448), has been withdrawn by agreement between the authors, the journal Editor in Chief William M. Worek and Wiley Periodicals LLC.The withdrawal has been agreed due to a technical error at the publisher that caused the article to be mistakenly published online although its publication had been cancelled at the request of the authors.
Aggregation propensities of proteins with varying degrees of disorder
Authors: Dey Priya , Biswas Parbati 
Published: December 2022

AbstractThe hydration thermodynamics of a globular protein (AcP), three intrinsically disordered protein regions (1CD3, 1MVF, 1F0R) and a fully disordered protein (α‐synuclein) is studied by an approach that combines an all‐atom explicit water molecular dynamics simulations and three‐dimensional reference interaction site model (3D‐RISM) theory. The variation in hydration free energy with percentage disorder of the selected proteins is investigated through its nonelectrostatic and electrostatic components. A decrease in hydration free energy is observed with an increase in percentage disorder, indicating favorable interactions of the disordered proteins with the solvent. This confirms the role of percentage disorder in determining the aggregation propensity of proteins which is measured in terms of the hydration free energy in addition to their respective mean net charge and mean hydrophobicity. The hydration free energy is decoupled into energetic and entropic terms. A residue‐wise decomposition analysis of the hydration free energy for the selected proteins is evaluated. The decomposition shows that the disordered regions contribute more than the ordered ones for the intrinsically disordered protein regions. The dominant role of electrostatic interactions is confirmed from the residue‐wise decomposition of the hydration free energy. The results depict that the negatively charged residues contribute more to the total hydration free energy for the proteins with negative mean net charge, while the positively charged residues contribute more for proteins with positive mean net charge.
Projective planes of order 12 do not have a collineation group of order 4
Authors: Akiyama Kenzi , Suetake Chihiro , Tanaka Masaki 
Published: December 2022

AbstractIn this paper, we prove that there are no projective planes of order 12 admitting a collineation group of order 4. This yields that the order of any collineation group of a projective plane of order 12 is 1, 2, or 3.
Implementing school‐wide sexual assault prevention in middle schools: A qualitative analysis of school stakeholder perspectives
Authors: Orchowski Lindsay M. , Oesterle Daniel W. , Zong Zoe Y. , Bogen Katherine W. , Elwy A. Rani , Berkowitz Alan D. , Pearlman Deborah N. 
Published: December 2022

AbstractSexual assault and harassment are significant problems that begin early in the lifespan. The current study sought to understand the contextual factors that influence the implementation of school‐wide sexual assault prevention programs in middle schools that focus on fostering community norms change. The Consolidated Framework for Implementation Research (CFIR) is a menu of constructs arranged across 5 domains that assists stakeholders in assessing and identifying site‐specific determinants of successful intervention implementation. In the present study, researchers conducted a series of 10 interviews with middle school stakeholders (i.e., principals, guidance counselors, teachers) to document characteristics inside and outside of the school environment (i.e., cultural norms, relative priority, prior interventions, implementation climate) relevant to the implementation of prevention programming, using the CFIR as a guiding framework for analysis. Whereas schools recognized the importance of implementing violence prevention programming, stakeholders reported several other competing demands (i.e., time, resources) that make it difficult to implement rigorous programming without support from an outside agency/team. Community agencies and research teams hoping to implement violence prevention in middle schools can benefit from using stakeholder interviews grounded in the CFIR model to gain a better awareness of the school‐ and community‐specific factors that are likely to influence successful implementation of violence prevention programs in middle schools.
Diagnostic reproducibility of the 2018 Classification of Gingival Recession Defects and Gingival Phenotype: A multicenter inter‐ and intra‐examiner agreement study
Authors: Pini Prato Giovanpaolo , Di Gianfilippo Riccardo , Pannuti Claudio M. , Allen Edward P. , Aroca Sofia , Avila‐Ortiz Gustavo , Bouchard Philippe , Cairo Francesco , Chackartchi Tali , Cortellini Pierpaolo , Franceschi Debora , Mahajan Ajay , Mancini Evelyn A. , McGuire Michael K. , Natsvlishvili Tea , Santamaria Mauro P. , Scheyer E. Todd , Wang Hom‐Lay , Kornman Kenneth , Chambrone Leandro 
Published: December 2022

AbstractBackgroundThe aim of this study was to investigate the inter‐ and intra‐examiner agreement among international experts on the diagnosis of gingival recession defects using the 2018 Classification of Gingival Recession Defects and Gingival Phenotype as proposed in the 2017 World Workshop.MethodsStandardized intraoral photographs from 28 gingival recession defects were evaluated twice by 16 expert periodontists. Recession type (RT), recession depth (RD), keratinized tissue width (KTW), gingival thickness (GT), detectability of the cemento‐enamel junction (CEJ), and presence of root steps (RS) were recorded and used for the analysis. Intra‐ and inter‐examiner agreements were calculated for individual variables and for the overall classification. Intraclass correlation coefficient with 95% CI was used for RD and KTW; Kappa with 95% CI was used for GT, CEJ, and RS; quadratic weighted Kappa with 95% CI was used for RT.ResultsOverall intra‐ and inter‐examiner agreements were highest for KTW (0.95 and 0.90), lowest for GT (0.75 and 0.41), with the other variables in between (RD: 0.93 and 0.68, RS: 0.87 and 0.65, RT: 0.79 and 0.64, CEJ: 0.75 and 0.57). Overall intra‐ and inter‐examiner agreements for the matrix were 62% and 28%, respectively. Significant effects existed between one variable's measurement and other variables’ agreements.ConclusionsThe 2018 Classification of Gingival Recession Defects and Gingival Phenotype is clinically reproducible within the examiners, and when the variables forming the matrix are analyzed individually. The between‐examiner agreement for the complete matrix showed lower reproducibility. The agreement was highest for KTW and RD, and least for GT.
KIF4A promotes genomic stability and progression of endometrial cancer through regulation of TPX2 protein degradation
Authors: Zhang Jun , An Lanfen , Zhao Rong , Shi Rui , Zhou Xing , Wei Sitian , Zhang Qi , Zhang Tangansu , Feng Dilu , Yu Zhicheng , Wang Hongbo 
Published: December 2022

AbstractKinesin family member 4A (KIF4A) belongs to the kinesin superfamily proteins, which are closely associated with mitophagy. Nonetheless, the role of KIF4A in endometrial cancer (EC) remains poorly characterized. The present study showed that KIF4A not only was upregulated but also predicted poor prognosis in patients with EC. KIF4A knockdown in EC cells resulted in attenuated proliferative capacity in vitro and in vivo. Transcriptome sequencing and gene function analysis revealed that KIF4A contributed to the maintenance of EC cells' genomic stability and that KIF4A knockdown induced the DNA damage response, cell cycle arrest, and apoptosis. Mechanistically, KIF4A interacted with TPX2 (a protein involved in DNA damage repair to cope with the replication pressure) to enhance its stability via inhibition of TPX2 ubiquitination and eventually ensured the genomic stability of EC cells during mitosis. Taken together, our results indicated that KIF4A functions as a tumor oncogene that facilitates EC progression via the maintenance of genomic stability. Therefore, targeting the KIF4A/TPX2 axis may provide new concepts and strategies for the treatment of patients with EC.