bluuebunny/crossref_metadata_2025_split huggingface dataset:
LANA regulates miR‐155/GATA3 signaling axis by enhancing c‐Jun/c‐Fos interaction to promote the proliferation and migration of KSHV‐infected cells
Authors: Luo Ting , Pan Yangyang , Liu Yuhao , Zheng Jun , Zhuang Zhaowei , Ren Zuodong , Zhu Jiaojiao , Gu Yongqing , Zeng Yan 
Published: October 2022

AbstractKaposi's sarcoma (KS) is the second most common tumor in people infected with human immunodeficiency virus worldwide, but its pathogenesis is still unclear. In this study, we discovered that the expression of GATA‐binding protein 3 (GATA3) was lowly expressed in KS tissues and KSHV‐infected cells, while microRNA‐155 (miR‐155) was highly expressed in KS serum and KSHV‐infected cells. miR‐155 promoted the proliferation, migration and invasion of KSHV infection by targeting GATA3. Further, The KSHV‐encoded protein, the Latency associated nuclear antigen (LANA), promotes the proliferation, migration and invasion of KSHV‐infected cells by regulating the miR‐155/GATA3 axis. Regarding the molecular mechanism, c‐Jun and c‐Fos interact to form a complex. LANA upregulates the expression of c‐Jun and c‐Fos and enhances the formation of c‐Jun/c‐Fos complex. The complex binds to the −95∼−100 bp site of miR‐155 promoter and transcriptionally activates miR‐155. All in all, LANA enhances the c‐Jun/c‐Fos interaction, resulting in enhanced transcriptional regulation of miR‐155 by the c‐Jun/c‐Fos complex, thereby downregulating GATA3 and promoting the proliferation, migration and invasion of KSHV‐infected cells. The discovery of LANA/c‐Jun/c‐Fos/miR‐155/GATA3 further refines the pathogenesis of KS, potentially opening a new avenue for developing effective drugs against KS.
Effective bioremediation of petroleum‐contaminated saline soil using halotolerant Bacillus strains isolated from the Persian Gulf
Authors: Ghaedrahmat Zeinab , Ghafari Shokouh , Almasi Halime , Baboli Zeynab , Neisi Abdolkazem 
Published: October 2022

AbstractA consortium isolated from the Persian Gulf is evaluated for its ability to bioremediate petroleum‐contaminated soils. The soil sample was collected from oil fields of South Western Iran. The crude oil concentrations were set to 1000–10,000 mg/kg, and the sodium chloride concentration was set to 0.5%, 1%, 1.5%, 2%, and 2.5%. Operational parameters including volume (2–20 ml) and soil moisture (25%, 50%, and 100%) were studied consecutively according to one factor at the time of experimental design. A total number of eight different isolates capable of degrading crude oil were isolated from hydrocarbon‐contaminated sites (KL1–KL8). The removal efficiency of Total petroleom hydrocarbons (TPH) with an initial concentration of 1000 mg/kg for numbers of bacterial cells per gram soil of 2, 10, and 20 CFU/g was 20.9%, 45%, and 60%, respectively. The removal efficiency of TPHs (initial concentration of 1000 mg/kg) at the end of fifth week for salinity amounts of 0.5%, 1%, 1.5%, 2%, and 2.5% was 10.87%, 22.4%, 25.7%, 68.6%, and 60.5%, respectively. The TPHs biodegradation efficiencies at different soil/water ratios of 25%, 50%, and 100% (slurry) were 12%, 28.7%, and 60.8%, respectively. In sunflowers, there was no statistically significant difference in seed germination for different levels of soil pollution (p > 0.05). The results of the current work suggest that this process is a viable and efficient method for remediating contaminated sites. To enhance the removal results in real soil, a scale‐up study should also be conducted.
Understanding experiential awareness in humanistic‐phenomenological counseling
Authors: Wilkinson Brett D. 
Published: October 2022

AbstractThe author distinguishes metacognition from experiential awareness in counseling. While all therapeutic approaches engage metacognitive awareness, phenomenologically driven humanistic approaches emphasize experiential awareness by attending to the subjective character of mental acts and the qualitative character of experience. Clinical implications are examined to illustrate how experientially focused practices differ from rationality‐focused practices.
SE‐ResNet‐based classifier for highly similar mixtures based on Raman spectrum: Classification for alcohol systems as an example
Authors: Xie Yuhao , Yang Siwei , Zhou Shenghua , Liu Jiazhen , Zhao Shuai , Jin Shangzhong , Chen Qiang , Liang Pei 
Published: October 2022

AbstractRaman spectroscopy is widely used in the identification of substances. Raman spectra contain molecular information from various components and interference from noise and instruments. Therefore, using Raman spectroscopy to identify components is still challenging, especially for substances with high similarity. In this study, a class of highly similar products (methanol and ethanol) and a multicomponent mixture of propanol and water were tested using a portable Raman spectrometer. They are divided into 11 categories according to the volume fraction ratio. A total of 5,060 groups of Raman spectrum data were obtained, constituting the data set of this study. The deep neural network structure adopted in this study is based on ResNet architecture, on which the SE module in the attention mechanism is added, which increases the weight of some spectral features and achieves significant performance improvement. Finally, SE‐ResNet achieves a recall of 0.95, a precision rate of 0.95, a Micro‐F1 of 0.95, and an accuracy rate of 99.20%.
Ecofriendly chromatographic estimation of spasmolytic pharmaceutical mixture along with official toxic impurity, 3,5‐dichloroaniline: Complete green profile appraisal
Authors: Darweish Eman , Marzouk Hoda M. , Fayez Yasmin M. , Eissa Maya S. 
Published: October 2022

Nowadays, Green Analytical Chemistry is widely applied to provide various analytical methods with eco‐friendly procedures employing the least toxic, harmful reagents on humans and the environment without affecting the efficacy of the determination. Accordingly, two eco‐friendly, accurate, and reliable high‐performance thin‐layer chromatography‐densitometry and high‐performance liquid chromatographic methods were established for the determination and separation of two antispasmodic drugs, namely phloroglucinol and trimethylphloroglucinol in their pure, combined dosage form along with phloroglucinol toxic impurity, 3,5‐dichloroaniline. For high‐performance thin‐layer chromatography‐densitometry, efficient separation was developed via utilizing the stationary phase of high‐performance thin‐layer chromatography silica gel 60 F254 plates and developing a system comprising of ethyl acetate‐butanol‐ammonia in the ratio of 8.0:2.0:0.2, by volume and scanning of the developed bands at 210.0 nm. The subsequent method is isocratic high‐performance liquid chromatography with diode array detection in which separation was successively attained using XTerra RP‐C18 (250 × 4.6 mm, 5 μm) column as stationary phase and methanol‐10.0 mM phosphate buffer, pH 3.7 ± 0.1 as mobile phase in the ratio of 75.0:25.0, v/v at flow rate 1.0 ml/min and scanning at 220.0 nm. The developed liquid chromatography methods were validated according to the International Council for Harmonization guidelines, and all results acknowledged their efficacy. Additionally, the proposed methods worked well for assessing the cited drugs in binary combined commercially available pharmaceutical formulation. The greenness profile of the present methods was assessed and estimated using various assessment tools, namely; Green Analytical Procedure Index, analytical eco‐scale method, National Environmental Method Index in addition to Analytical GREEnness tool to evaluate the greenness of the provided methods with a statistical comparison between them to assess the more green ones.
Atmospheric chemistry of CCl2FCH2CF3(HCFC‐234fb): Kinetics and mechanism of reactions with Cl atoms and OH radicals
Authors: Sulbaek Andersen Mads P. , Frausig Morten , Nielsen Ole John 
Published: October 2022

AbstractThe atmospheric chemistry of CCl2FCH2CF3(HFCF‐234fb) was examined using FT‐IR/relative‐rate methods. Hydroxyl radical and chlorine atom rate coefficients ofk(CCl2FCH2CF3+OH)= (2.9 ± 0.8) × 10−15cm3molecule–1s–1andk(CCl2FCH2CF3+Cl)= (2.3 ± 0.6) × 10−17cm3molecule–1s–1were determined at 297 ± 2 K. The OH rate coefficient determined here is two times higher than the previous literature value. The atmospheric lifetime for CCl2FCH2CF3with respect to reaction with OH radicals is approximately 21 years using the OH rate coefficient determined in this work, estimated Arrhenius parameters and scaling it to the atmospheric lifetime of CH3CCl3. The chlorine atom initiated oxidation of CCl2FCH2CF3gives C(O)F2and C(O)ClF as stable secondary products. The halogenated carbon balance is close to 80% in our system. The integrated IR absorption cross‐section for CCl2FCH2CF3is 1.87 × 10−16 cm molecule−1(600–1600 cm−1) and the radiative efficiency was calculated to 0.26 W m−2ppb1. A 100‐year Global Warming Potential (GWP) of 1460 was determined, accounting for an estimated stratospheric lifetime of 58 years and using a lifetime‐corrected radiative efficiency estimation.
Knowledge sharing, knowledge seeking, and emotions: A longitudinal study of hospital restructuring decision making
Authors: Chan Yolande E. , Krishnamurthy Rashmi , Mann Janelle , Sabherwal Rajiv 
Published: October 2022

AbstractPrior research examines factors that enable or hinder knowledge sharing and knowledge seeking in groups. However, individuals also share and seek knowledge outside group meetings, especially if the group is making strategic decisions over time. Therefore, this study examines how, during a longitudinal strategic decision‐making process, the emotions of group members and knowledge sharing within the group affect their knowledge‐sharing intentions and knowledge‐seeking behaviors beyond the group. We focus on a single organization, a hospital, whose board created a citizen advisory panel (CAP) of 28 individuals to gather community input on the restructuring of the hospital's activities to contain costs. The group met in five all‐day sessions to provide their input. We surveyed each member before the CAP process and after each CAP session. The resulting longitudinal data were analyzed using panel‐data techniques, with the findings being complemented by qualitative insights. The results indicate, somewhat surprisingly, that both positive and negative emotions (specifically enthusiasm and anxiety) positively affect both knowledge‐sharing intentions and knowledge sharing within groups in strategic decision‐making contexts. We also find that enthusiasm, anxiety, and perceived relative knowledge within groups positively affect subsequent knowledge‐seeking behaviors. Our findings contribute to the literature on knowledge management and organizational decision making. The study provides insights into how, in groups making strategic decisions over time, emotions as well as knowledge sharing within groups affect knowledge‐related intentions and behaviors beyond the groups. The study also adds to the theory of planned behavior to highlight the role of emotions in influencing intentions and behaviors.
Impact of Serial Intralesional Steroid Injections on Idiopathic Subglottic Stenosis
Authors: Hoffman Matthew R. , Patro Ankita , Huang Li‐Ching , Chen Sheau‐Chiann , Berry Lynn D. , Gelbard Alexander , Francis David O. ,   North American Airway Collaborative
Published: October 2022

ObjectivesSerial intralesional steroid injection (SILSI) has been increasingly used to treat idiopathic subglottic stenosis (iSGS). Prior studies have shown effectiveness, but not in all patients. This multi‐institutional study evaluates the effect of SILSI on time to recurrent operation, peak expiratory flow (PEF), and dyspnea.MethodsPost‐hoc secondary analysis of the North American Airway Collaborative data were performed to evaluate the outcomes of iSGS patients undergoing and not undergoing SILSI. The primary outcome was time to recurrent operation, evaluated using Kaplan–Meier curves and Cox regression analysis. Secondary outcomes were change in PEF and clinical chronic obstructive pulmonary disease questionnaire (CCQ) score. Within patients undergoing SILSI, demographics, time from last procedure, and PEF at initiation of SILSI were evaluated to determine the effect on recurrence.ResultsTwo hundred and ninety patients were included, 238 undergoing endoscopic dilation alone and 52 undergoing dilation and SILSI. No differences in baseline characteristics were observed. There was no difference in time to recurrence (hazard ratio: 0.64; p = 0.183). There were no differences in PEF or CCQ across the 2.5‐year study period. Among 52 patients undergoing SILSI, PEF at the time of starting SILSI did not affect recurrence (χ2 = 0.09, p = 0.77).ConclusionPatients undergoing and not undergoing SILSI had similar times to recurrence, PEF, and CCQ. Factors predicting recurrence among patients undergoing SILSI were not identified. These results support a randomized controlled trial with a uniform SILSI protocol to quantify the effects of SILSI on objective and subjective outcomes and help determine which iSGS patients benefit most.Level of Evidence3 Laryngoscope, 133:2255–2263, 2023
Gender Disparities in Otolaryngology: A Case Report Reflecting Global Perspectives
Authors: Ali Hawa M. , Lee Stellina Y. H. , Jashek‐Ahmed Farizeh , Seguya Amina , Faucett Erynne , Van Beck Jenna , Stapleton Emma , Peer Shazia , Husain Inna A. , Wiedermann Joshua , Salano Valerie ,   Global OHNS
Published: October 2022

ObjectivesTo explore the impact of female sex on the experiences of trainees and surgeons in otolaryngology from LMIC and HIC.MethodsThis study includes perspectives of five consultants and two resident physicians from the United States, United Kingdom, Uganda, Kenya and South Africa.ResultsSix themes emerged from these interviews. Overall, LMIC and HIC women shared similar experiences of microaggressions during patients, working along ancillary staff, related to pregnancy, imposter syndrome, difficulties during job search, and unique barriers as consultant.ConclusionsThe findings of this study highlight that gender disparities are present at all levels in Otolaryngology but can present differently depending on context. Laryngoscope, 133:547–551, 2023
Application of Machine Learning to Predict Hearing Outcomes of Tympanoplasty
Authors: Koyama Hajime , Kashio Akinori , Uranaka Tsukasa , Matsumoto Yu , Yamasoba Tatsuya 
Published: October 2022

ObjectiveThis retrospective study aimed to evaluate the performance of machine learning techniques in predicting air‐bone gap after tympanoplasty compared with conventional scoring models and to identify the influential factors.MethodsWe reviewed the charts of 105 patients (114 ears) with chronic otitis media who underwent tympanoplasty. Two numerical scoring systems (middle ear risk index [MERI] and ossiculoplasty outcome parameter staging [OOPS]) and three algorithms (random forest [RF], support vector machine [SVM], and k nearest neighbor [kNN]) were created. Experimental variables included age, preoperative air‐bone gap, soft‐tissue density lesion in the tympanic cavity in CT, otorrhea, surgical history, ossicular bone problems in CT, tympanic perforation location, perforation type (central or marginal), grafting material, smoking history, endoscopy use, and the operator whose experience was 20 years or longer, or shorter. Binary classification, postoperative air‐bone gap ≤15 or >15 dB, and multiclass classification, classification into seven categories by 10 dB, were performed, and the percentages of correct prediction were calculated. The importance of features in the RF model was calculated to identify influential factors.ResultsThe percentages of correct prediction in binary classification were 62.3%, 72.8%, 81.5%, 81.5%, and 81.5% in MERI, OOPS, RF, SVM, and kNN, respectively, and those in multiclass classification were 29.8%, 21.9%, 63.1%, 44.7%, and 50% in the same order. The RF model suggested larger preoperative air‐bone gap, and older age could make the postoperative air‐bone gap larger.ConclusionThe machine learning techniques, especially the RF model, are promising methods for precise postoperative air‐bone gap prediction.Level of Evidence4 Laryngoscope, 133:2371–2378, 2023
Sodium Phytate‐Incorporated Gelatin‐Silicate Nanoplatelet Composites for Enhanced Cohesion and Hemostatic Function of Shear‐Thinning Biomaterials
Authors: Zehtabi Fatemeh , Montazerian Hossein , Haghniaz Reihaneh , Tseng Kaylee , Mohaghegh Neda , Mandal Kalpana , Zamanian Behnam , Dokmeci Mehmet Remzi , Akbari Mohsen , Hassani Najafabadi Alireza , Kim Han‐Jun , Khademhosseini Ali 
Published: October 2022

AbstractShear‐thinning biomaterials (STBs) based on gelatin‐silicate nanoplatelets (SNs) are emerging as an alternative to conventional coiling and clipping techniques in the treatment of vascular anomalies. Improvements in the cohesion of STB hydrogels pave the way toward their translational application in minimally invasive therapies such as endovascular embolization repair. In the present study, sodium phytate (Phyt) additives are used to tune the electrostatic network of SNs‐gelatin STBs, thereby promoting their mechanical integrity and facilitating injectability through standard catheters. We show that an optimized amount of Phyt enhances storage modulus by approximately one order of magnitude and reduces injection force by ≈58% without compromising biocompatibility and hydrogel wet stability. The Phyt additives are found to decrease the immune responses induced by SNs. In vitro embolization experiments suggest a significantly lower rate of failure in Phyt‐incorporated STBs than in control groups. Furthermore, the addition of Phyt leads to accelerated blood coagulation (reduces clotting time by ≈45% compared to controls) due to the contributions of negatively charged phosphate groups, which aid in the prolonged durability of STB in coagulopathic patients. Therefore, the proposed approach is an effective method for the design of robust and injectable STBs for minimally invasive treatment of vascular malformations.
Evaluation of nitrogen content on pitting and repassivation behavior of a high alloy nickel‐reduced austenitic stainless steel
Authors: Hempel Christian , Mandel Marcel , Quitzke Caroline , Schröder Christina , Wendler Marco , Volkova Olena , Krüger Lutz 
Published: October 2022

AbstractThe present study deals with the pitting and repassivation behavior of a nickel‐reduced austenitic stainless steel with different nitrogen contents. While nitrogen promoted the resistance to pitting, no significant effect on repassivation was found. The study also aims to address the experimental difficulties in determining repassivation potentials owing to the occurrence of crevice corrosion. Furthermore, the mechanism of the ongoing reactions was investigated, indicating that pitting was initiated in regions where manganese sulfide inclusions were present next to the remaining δ‐ferrite.
Mechanism of surface corrosion resistance of 304 stainless steel processed by nanosecond laser pulses
Authors: Yang Qibiao , Wang Dongguan , Xiong Yihao , Zhang Daomeng , Chen Lie , Cheng Jian , Lou Deyuan , Liu Dun 
Published: October 2022

AbstractA nanosecond pulsed fiber laser was used to modify the surface of 304 stainless steel. The compactness, microstructure, chemical composition, and corrosion resistance of the stainless steel passive film were observed by the blue dot detection method, scanning electron microscopy, x‐ray photoelectron spectroscopy, and electrochemical methods. The influence of different laser fluences on the stability and corrosion resistance of the stainless steel passive film was studied. The results show that with increasing laser fluence, the discoloration reaction time of the stainless steel surface increases first and then decreases, while the surface roughness decreases first and then increases. When the laser fluence is lower than 11.19 J/cm2, a compact and uniform passive film is formed. When the laser fluence is larger than this value, the stainless steel substrate is prone to secondary corrosion. Chromium oxides are enriched in the passive film, and the anodic dissolution rate decreases, which promotes a positive shift in the corrosion potential of the stainless steel, decreases the corrosion current density and increases the polarization resistance and the corrosion resistance. The surface corrosion resistance is thus significantly improved.
A Phenylene‐Based Light‐Emitting Polymer: Atums Green
Authors: Mehreen Tanisha , Vagin Sergey I. , Kumar Vishal , Liu Xiaoyuan , Eylert Lukas , Rieger Bernhard , Meldrum Alkiviathes 
Published: October 2022

AbstractThis work synthesizes a green‐fluorescent conjugated polymer and performs basic photophysical characterization of this new material. Atums Green[1] is synthesized by a Suzuki cross‐coupling polymerization reaction between isostructural dibromo and diboronic acid monomers and is structurally characterized by nuclear magnetic resonance and gel permeation chromatography. The polymer consists of an alkoxy‐substituted 1,4‐bis((E)‐styryl)benzene repeating unit with molecular weight up to Mn = 50 kDa relative to polystyrene. Atums Green shows a strong green fluorescence maximized at ≈500 nm in chloroform and tetrahydrofuran solutions, with an absolute quantum efficiency as high as 98%. The photobleaching dynamics and time‐resolved photoluminescence (TRPL) are measured both in solutions and in solid films. Finally, solution‐based lasing is demonstrated in a bulk lasing cavity and in a cylindrical microcavity. Lasing emission is readily achieved in both formats, indicating that Atums Green has excellent emission characteristics, and further suggesting that it may present a viable green‐emitting conjugated polymer materials system for light emission applications.
Whole‐genome sequencing and genome‐scale metabolic modeling of Chromohalobacter canadensis 85B to explore its salt tolerance and biotechnological use
Authors: Enuh Blaise Manga , Nural Yaman Belma , Tarzi Chaimaa , Aytar Çelik Pınar , Mutlu Mehmet Burçin , Angione Claudio 
Published: October 2022

AbstractSalt tolerant organisms are increasingly being used for the industrial production of high‐value biomolecules due to their better adaptability compared to mesophiles. Chromohalobacter canadensis is one of the early halophiles to show promising biotechnology potential, which has not been explored to date. Advanced high throughput technologies such as whole‐genome sequencing allow in‐depth insight into the potential of organisms while at the frontiers of systems biology. At the same time, genome‐scale metabolic models (GEMs) enable phenotype predictions through a mechanistic representation of metabolism. Here, we sequence and analyze the genome of C. canadensis 85B, and we use it to reconstruct a GEM. We then analyze the GEM using flux balance analysis and validate it against literature data on C. canadensis. We show that C. canadensis 85B is a metabolically versatile organism with many features for stress and osmotic adaptation. Pathways to produce ectoine and polyhydroxybutyrates were also predicted. The GEM reveals the ability to grow on several carbon sources in a minimal medium and reproduce osmoadaptation phenotypes. Overall, this study reveals insights from the genome of C. canadensis 85B, providing genomic data and a draft GEM that will serve as the first steps towards a better understanding of its metabolism, for novel applications in industrial biotechnology.