bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a glutamate-cysteine ligase in Bombyx mori
Authors: Afrin Wazifa , Furuya Shigeki , Yamamoto Kohji 
Published: October 2022

Abstract
Background: Glutamate cysteine ligase (GCL) is a crucial enzyme involved in the synthesis of glutathione (GSH). Despite various studies on glutathione transferase and its essential role in detoxification and resistance to oxidative stress, GSH synthesis has not been described in silkworms to date. Silkworms form part of the lepidopterans that are considered as a model of agricultural pests. This study aimed to understand the GSH synthesis through the GCL in silkworms so that insecticides can be formulated to tackle agricultural pests.
Methods and Results: Based on the amino acid sequence and phylogenetic tree, this GCL belonged to group 2 and designated bmGCL. Recombinant bmGCL was overproduced and purified to ensure homogeneity. Biochemical studies revealed that bmGCL uses ATP and Mg2+ to ligate glutamate and cysteine, respectively. High expression levels of bmgcl mRNA and GSH were observed in the silkworm fat body after exposure to insecticides and UV-B. Moreover, we found an increase in bmgcl mRNA and GSH content during pupation in the silkworm fat body.
Conclusion: In this study, we found GCL in the silkworm Bombyx mori and its biochemical properties were investigated. These observations indicate that bmGCL might play an important role in the resistance to oxidative stress in B. mori.
Explore the Diagnostic Performance of 2020 C-TIRADS by Comparing With the 2017 ACR-TIRADS guidelines: A Single-Center Study
Authors: cai Miaomiao , chen Libo , lv Xuan , shui Limin , wang Hui 
Published: October 2022

Abstract
Objective
To compare the diagnostic efficacy of the Chinese Thyroid Imaging Reporting and Data Systems(C-TIRADS) with the well-accepted ACR-TIRADS guidelines in identifying benign from malignant thyroid nodules.
Methods
A total of 2064 nodules were collected from 1627 patients undergoing thyroid ultrasonography in our center between October 2019 to November 2021. Nodules were divided into two groups: “≥1cm”and “<1cm”. Ultrasound features of each nodule were observed and recorded by 2 physicians and classified according to the ACR-TIRADS and C-TIRADS guidelines, respectively.
Results
The area under the curve(AUC) of the ACR-TIRADS guideline was higher than that of the C-TIRADS guideline(0.922, P = 0.017), the specificity and positive predictive value of the C-TIRADS guideline were higher (81.64%, 88.72%, all P < 0.05). The diagnostic performance showed similar results for nodules < 1cm. Additionally, the diagnostic efficacy of both guidelines was comparable in nodules ≥ 1cm. The ACR-TIRADS effectively reduced unnecessary biopsies compared with the C-TIRADS (P < 0.05).
Conclusions
There was high agreement between two guidelines for the diagnosis of thyroid nodules, C-TIRADS guidelines had a higher specificity and simplicity while were inferior to the ACR-TIRADS guidelines in terms of reducing the number of biopsies.
Validity and reliability of “My Jump app” to assess vertical jump performance: A meta-analytic review
Authors: Turan Murat , Ulupınar Süleyman , Özbay Serhat , Gençoğlu Cebrail , Savaş Buğra Çağatay , İnce İzzet 
Published: October 2022

Abstract
This systematic review and meta-analysis aims to investigate whether the My Jump app produces valid and reliable results when measuring vertical jump height. To identify potential studies for inclusion in this systematic review and meta-analysis, a search strategy was implemented in the MEDLINE (via PubMed) and Web of Science databases. Meta-analyses of validity were performed in two ways: (1) mean and standard deviations were compared between the My Jump and criterion measurements to assess the agreement of raw scores; (2) correlation coefficients were used to determine the consistency of the rankings within-group in the My Jump and criterion measurements. The meta-analysis of reliability was conducted using intraclass correlation coefficients (ICC). Heterogeneity was determined by Cochrane’s Q statistic and its p-value, I-squared value, and tau-squared value. The risk of publication bias was explored using funnel plot symmetry, and asymmetries were confirmed using the extended Egger’s test. Following the identification of studies providing inclusion criteria, 12 studies were included in the present meta-analysis. The meta-analysis conducted for identifying the agreement between raw scores showed that there was no significant difference between My Jump and the criterion measurements. The meta-analysis for identifying the consistency of the rankings within-group indicated that there was a high correlation between My Jump and criterion devices. Additionally, it is found that the My Jump app had nearly perfect reliability scores. Consequently, this study showed that the My Jump app presented high agreement and consistency scores with reference methods.
Dielectrophoresis-driven Single-cell Manipulation on Microelectrode Array Chips for Accurate Intercellular Interaction Study
Authors: Sawan Mohamad , Zhang Hongyong , Wang Pengbo , Huang Nan , Zhao Lingrui , Su Yi , Li Lingfei , Bian Sumin 
Published: October 2022

Abstract
Single-cell manipulation chips are considered powerful platforms to analyze intercellular interactions and cellular function at single-cell level. Although the conventional cell co-culture models could investigate cell communication to some extent, the role of a single cell requires further analysis. This study focuses on the design and fabrication of a single-cell manipulation platform based on microelectrode array (MEA) chips using dielectrophoresis to build a precise intercellular interaction model on the chip. Single cells were trapped on or transferred between electrodes to achieve exact manipulation. Each electrode on the MEA chip was independently controlled, and the corresponding cellular electrophysiology could be recorded. Multiple influencing factors, including the diameter and depth of microwells, geometry of cells, and voltage amplitude of the control signal, were investigated to determine the optimal condition for cell manipulation. Two typical types of cells, 293T cells and neural cells, were used to test the chip and investigate the influence of electric field on cells. Meanwhile, cell morphology was continuously monitored under microscope. The inappropriate use of the electric field on cells was examined based on the oncosis phenomenon. The obtained preliminary results extended the nature growing model to the controllable level, satisfying the expectation of introducing more elaborated intercellular interaction models.
A Chinese case of CHST3-related skeletal dysplasia and a systematic review
Authors: Liang Hanting , Qi Wenting , Jin Chenxi , Pang Qianqian , Cui Lijia , Jiang Yan , Wang Ou , Li Mei , Xing Xiaoping , Liu Wei , Xia Weibo 
Published: October 2022

Abstract
Purpose
This study described a case with carbohydrate sulfotransferase 3 (CHST3) spondyloepiphyseal dysplasia and summarized all previously reported cases with CHST3-related skeletal dysplasia.
Methods
A 14.8-year-old boy underwent clinical and radiological evaluations, including high-resolution peripheral quantitative computed tomography. The patient and the family members underwent genetic tests. All CHST3-related skeletal dysplasia cases from PubMed and Embase were collected and analysed.
Results
The proband was found to have short lower limbs during a prenatal examination. At 11 years old, he had a compression fracture of L2. Since 13 years of age, he has complained of aggravated pain in the large joints. Physical examination showed a height Z score of -4.94, short limbs, and restricted movement of the elbows and knees. X-rays showed epiphyseal dysplasia of the carpal bones, enlargement of the left elbow and knee joints, and subluxation of the left hip. Echocardiography showed abnormal cardiac valves. Compared with the norm, his total and trabecular volumetric bone mineral density (vBMD) were significantly lower, and the microarchitecture of the trabecular bone was poor at the distal radius and tibia. Two novel missense variants of c.1343T > G and c.761C > G in CHST3 were inherited from his father and mother, respectively. In the systematic review, short stature, limited joint extension, joint pain, and joint dislocation were the most common characteristics associated with mutations of CHST3. Over 90% of pathogenic variants are located in the sulfotransferase domain.
Conclusion
This patient with CHST3-related skeletal dysplasia has progressive joint pain and movement restriction, poor vBMD, and abnormalities of the microarchitecture of the trabecular bone. There is no apparent genotype-phenotype correlation in this disorder.
In vitro and cutting propagation of Heuchera: rapid mass production protocol and correlation between initiating ability the two methods
Authors: Xu Chan , Guo Hang , Wang Zhijing , Chen Yuan 
Published: October 2022

Abstract
There is still a lack of understanding about the efficient mass propagation of multiple Heuchera cultivars leading to a large market gap. And there has been no empirical research on the relationship between propagation methods of cutting and in vitro. In this study, thirteen different cultivars from the genus Heuchera and Heucherella (Heuchera × Tiarella) were successfully rooted by cutting, and calluses and shoots were also successfully inducted from petiole in vitro culture. However, measured indexes varied significantly among cultivars for cutting and tissue culture. Petiole explants treated with cytokinin 6-benzylaminopurine (BA: 0.5 ~ 2.0 mg/L) plus auxin α-naphthaleneacetic acid (NAA: 0.5 mg/L) formed shoots either directly or indirectly during primary culture. And the dark treatment and 2,4-dichlorophenoxyacetic acid (2,4-D) were ineffective for shoot formation but beneficial for callus stimulation. Furthermore, we discovered a negative correlation between initiating cutting recovery and tissue culture inducting, implying that cultivars with low difficulty in cutting initiating were relatively difficult to tissue culture. Then, we developed a simple and efficient Heuchera rapid propagation protocol. That is, micropropagation through obtaining adventitious shoots directly from primary cultures, supplemented by cutting propagation. Our petiole explants system and leaf-cutting technique are expected to guide Heuchera's mass production.
The deep-plane facelift as a treatment modality for antiretroviral therapy induced cervicofacial lipodystrophy
Authors: Kustermans Lise , Genechten Maarten Van , de Casteele Elke Van , De Vos Wouter 
Published: October 2022

Abstract
PURPOSE
Highly Active Anti Retroviral Therapy (HAART) has become an effective treatment modality to reduce HIV-infection to an undetectable viral load. These antiretroviral agents may, however, cause severe morbidity by inducing the syndrome of lipodystrophy. In the head and neck region antiretroviral agents generate an obtuse heavy neck in combination with an accelerated pattern of midfacial atrophy and ptosis. Modifying the antiretroviral therapy produces only limited improvement, therefore warranting a surgical approach. An extreme case of HAART-induced facial lipodystrophy is presented together with literature review to support the need for an extended surgical intervention in these patients.
METHODS
An overview of literature concerning the lipodystrophy effects of HAART and potential treatment modalities is carried out. This is illustrated with a case study of a 55-year old woman who received HAART therapy for 24 years and was particularly unhappy with her appearance due to cervical fat accumulation. Surgical technique and clinical results in this patient by means of deep-plane cervico-facial rhytidectomy including liposuction, subplatysmal lipectomy and platysma-digastric suspension are discussed.
RESULTS
The surgical technique has resulted in an aesthetically pleasing and predictable result with a quick recovery in this patient suffering from HAART-induced lipodystrophy.
CONCLUSION
The technique of deep-plane cervico-facial rhytidectomy including liposuction, subplatysmal lipectomy and platysma-digastric suspension is a valuable treatment modality in this patient group suffering from HAART-induced facial lipodystrophy. However, longitudinal studies of multiple subjects is required to assess stability of the outcome.
Patterns of influence of different landslide boundaries and their spatial shapes on the uncertainty of landslide susceptibility prediction
Authors: Xing Yin , Huang Saipeng , Yue Jianping , Chen Yang , Xie Wei , Wang Peng , Xiang Yunfei , Peng Yiqun 
Published: October 2022

Abstract
Landslide boundaries and their spatial shapes are usually presented as irregular polygonal surfaces such as semicircles and bumps, but some landslide susceptibility modelling uses idealized landslide points or buffer circles as landslide boundaries, bringing the uncertainty to susceptibility modelling. To study the influence of different landslide boundaries on modelling uncertainty, 362 landslides and 11 environmental factors in Ruijin City were selected, and established landslide boundaries and their frequency ratio correlations with environmental factors based on landslide points (Point), buffer circles (Circle) and accurately decoded and drawn polygons (Polygon), respectively. Afterwards, Deep Belief Network (DBN) and Random Forest (RF) were selected to construct models such as Point, Circle, Polygon-based DBN and RF. Finally, the modelling uncertainty analysis was carried out using receiver operating characteristic (ROC) accuracy, the distribution pattern of the susceptibility index and its variability. The results indicate that: (1) Using landslide points or buffer circles as landslide boundaries will increase modelling uncertainty, while using accurate landslide polygon boundaries is more effective in ensuring modelling accuracy and reliability. (2) The uncertainty pattern of landslide susceptibility modelling based on DBN and RF is the same, but the prediction accuracy of the RF model is lower than DBN, moreover, its uncertainty is higher than DBN. (3) The landslide susceptibility results obtained by using points and buffer circles as landslide boundaries can reflect the spatial distribution pattern of landslide probability in the study area as a whole and can use as an alternative solution in the absence of accurate landslide boundaries.
Deep learning model coupled with proteomics to detect yeast epidemic clones across multiple hospitals
Authors: Mohammad Noshine , Normand Anne Cécile , Nabet Cécile , Brossas Jean-Yves , Blaize Marion , Bonnal Christine , Fekkar Arnaud , Imbert Sébastien , Piarroux Renaud , Tannier Xavier 
Published: October 2022

Abstract
The ongoing worldwide emergence of epidemic azole-resistant Candida parapsilosis clones is a threat to human health. In order to monitor a clonal outbreak which involved 2 hospitals in Paris, we used a technology that most clinical laboratories are equipped with: Matrix-Assisted Laser Desorption-Ionization Time of Flight mass spectrometry (MALDI-TOF MS). This physico-chemical analysis technique was combined with machine learning algorithm to evaluate the possibility of monitoring a multi-center epidemic. A total of 96 isolates of Candida parapsilosis constituted of resistant clones, susceptible clones and other susceptible isolates were cultured on various culture conditions. Spectra were acquired on four different mass spectrometers from three hospitals. We used convolutional neural networks (CNN) to classify the isolates and identified the main parameters that can influence the identification of epidemic clonal isolates. We have shown results up to 94% accuracy in a multi-center context, and between 81 and 91% accuracy when the tested site is not part of the training set, showing that it is possible to differentiate the spectra of a clone and a non-clone presenting a very strong similarity. This study shows that it is possible to monitor a certain type of clone, namely the R2 clones in our case study, using MALDI-TOF coupled to CNN. The experimental conditions that impacted the results should be considered for future work to improve performances for difficult classification tasks like clonal fungal identification.
Synchrony to a beat predicts synchrony with other minds
Authors: Wohltjen Sophie , Toth Brigitta , Boncz Adam , Wheatley Thalia 
Published: October 2022

Abstract
Synchrony has been used to describe simple beat entrainment as well as correlated mental processes between people, leading some to question whether the term conflates distinct phenomena. Here we ask whether simple synchrony (beat entrainment) predicts more complex shared attentional states, consistent with a common mechanism. While eye-tracked, participants listened to regularly spaced tones and indicated changes in volume. Across multiple sessions, we found a reliable individual difference: some people entrained their attention more than others, as reflected in beat-matched pupil dilations that predicted performance. In a second study, eye-tracked participants completed the beat task and then listened to an eye-tracked storyteller. An individual’s tendency to entrain to a beat predicted how strongly their pupils synchronized with those of the storyteller, a measure of shared attention. The tendency to synchronize is a stable individual difference that predicts both beat entrainment and the coupling of minds, consistent with a common origin.
Correlation Between Mortality From Aortic Aneurysm, Sex, Administrative Regions and Age Group in Brazil
Authors: Santos Keller Da Silva , Galego Sidnei José , Lucena Diego Monteiro de Melo , Figueiredo Francisco Winter Dos Santos 
Published: October 2022

AbstractIntroduction Aneurysmal disease is a focal and permanent dilation of the vessel diameter greater than 50% of its normal diameter, whose natural history is the progressive enlargement of the aneurysm, culminating in its rupture, if not treated. Traditional risk factors are those common to other cardiovascular diseases. However, even though socioeconomic status has gained importance in terms of its influence on vascular diseases, few studies seek to understand the behavior of this disease in countries with high levels of socioeconomic inequality, such as Brazil. Objective to analyze the correlation between age group, sex, and mortality from aortic aneurysm in the Brazilian federative units. Method study with analysis of secondary data on mortality from Aortic Aneurysms in Brazilian states in the year 2019, extracted from the Global Burden of Disease platform and the Institute for Applied Economic Research. The program used for statistical analysis was Stata® (StataCorp, LC) version 11.0. Results Higher mortality was observed in males, with a statistically significant increase in mortality in age groups from 40 years onwards. There are also important regional differences in mortality from standardized Aortic Aneurysms in Brazilian administrative regions. Conclusion mortality from aortic aneurysm increases with age, with deaths being detected from the fourth decade of life onwards, although screening only occurs after 65 years of age. Such results may justify the need to include younger individuals, around 40 years of age, in the screening for aneurysm detection.
Influence of graft thickness on tunnel technique procedures for root coverage: A pilot split-mouth randomized controlled trial
Authors: Teodoro Victor Aguirre , Mattedi Marco Antonio Marques , Vergara-Buenaventura Andrea , Muniz Francisco Wilker Mustafa Gomes , Meza-Mauricio Jonathan , Faveri Marcelo , Ciotti Danilo Lazzari 
Published: October 2022

AbstractObjective:To compare the clinical outcomes of the tunnel technique (TUN) combined with either thin connective tissue graft (C.T.G.) (TUN + thin C.T.G.) (1 mm) or thick C.T.G. (TUN + thick C.T.G.) (2 mm) for the treatment of localized gingival recession (G.R.).Material and methods:Twelve patients, with 24 maxillary localized type RT1 GR were randomly assigned into two groups. Clinical, esthetic, and patient‐reported outcomes were evaluated at baseline and at 6-month follow-up.Results: The TUN+thin C.T.G. group showed a higher mean G.R. reduction (1.53± 0.66) (primary outcome variable) than the TUN+thick C.T.G. group (1.44± 0.89), but no statistically significant difference was detected at the 6-month follow-up (p=0.77). No statistically significant differences were found between TUN+thin C.T.G. and TUN+thick C.T.G. concerning complete root coverage (C.R.C.) (58.3% versus 41.7%, respectively), gingival ticknness (G.T.) or Root-coverage Esthetic Score (R.E.S.) at 6 months. Regarding patient‐reported outcomes, both groups reported having experienced similar pain at 7 days (p>0.05). No statistically significant differences were found between the groups in the analysis of the aesthetic score.Conclusion:Both treatments showed a similar reduction in G.R. In addition, similar C.R.C., G.T., R.E.S., pain and self-reported esthetics were achieved in both groups.Clinical relevance:Application of thin CTG provided a similar result than thick CTG for the treatment of RT1 localized GR.
TM4SF1 Upregulates MYH9 to Activate the NOTCH Pathway to Promote Cancer Stemness and Lenvatinib Resistance in HCC
Authors: Yang Si-bo , Lei Jin , Zhou Zi-han , Li Xiao-wen , Chen Qian , Li Bo , Zhang Ye-wei , Ge Yu-zhen , Zuo Shi 
Published: October 2022

Abstract
TM4SF1, a member of the transmembrane 4 superfamily, is crucial for both healthy and malignant human tissues. The significant function of TM4SF1 in the incidence and progression of cancer has been widely recognized in recent years. Although some achievements have been made in the study of TM4SF1, the effect of TM4SF1 on cancer stemness in hepatocellular carcinoma (HCC) and its molecular basis are yet to be reported. We found through abundant in vitro and in vivo experiments which the expression of TM4SF1 was positively correlated with the progression and cancer stemness of HCC. We identified the downstream protein MYH9 of TM4SF1 and its final regulatory target NOTCH pathway using bioinformatics analysis and protein mass spectrometry. We cultivated a lenvatinib-resistant strain from HCC cells to examine the relationship between cancer stemness and tumor drug resistance. The study confirmed that TM4SF1 could regulate the NOTCH pathway by upregulating MYH9, thus promoting cancer stemness and lenvatinib resistance in HCC. This study not only provided a new idea for the pathogenesis of HCC but also confirmed that TM4SF1 might become a new intervention point to improve the clinical efficacy of lenvatinib in treating HCC.