bluuebunny/crossref_metadata_2025_split huggingface dataset:
P‐body dynamics revealed by DDX6 protein knockdown via the auxin‐inducible degron system
Authors: Dhamodharan Adithya , Okada Hajime , Muraoka Masafumi , Wright Danelle , Okubo Akemi , Saga Yumiko 
Published: November 2022

AbstractThe transcriptome dynamically changes via several transcriptional and post‐transcriptional mechanisms. RNA‐binding proteins contribute to such mechanisms to regulate the cellular status. DDX6 is one such protein and a core component of processing bodies (P‐bodies), membrane‐less cytosolic substructures where RNA and proteins localize and are functionally regulated. Despite the importance of DDX6, owing to the lack of tightly controlled methods for protein knockdown, it was difficult to assess in high time resolution how its depletion exactly affects the P‐body assembly structure. Therefore, we adopted an advanced protein degradation method, the auxin‐induced degron (AID) system, to degrade DDX6 acutely in ES cells. By introducing AID‐tagged DDX6 and the E3 ligase subunit of OsTIR1 into ES cells, we successfully degraded DDX6 following auxin analog (indole‐3‐acetic acid, IAA) treatment. The degradation rate of DDX6 was slower than that of the cytosolic reporter protein EGFP but was enhanced by increasing the OsTIR1 dosage. Lastly, we confirmed that a substantial portion of P‐bodies disappears around the time of 1 hr after IAA addition consistent with DDX6 depletion detected by western blot. In accordance with this, we detected transcriptome changes by 6 hr after IAA treatment. Therefore, we demonstrated the applicability of the AID method to gain insight into the function of P‐bodies and their protein components.
Reputational recovery under political instability: Public debt in Portugal, 1641–83
Authors: Costa Leonor Freire , Miranda Susana Münch 
Published: November 2022

AbstractThis article examines the reputation recovery of Portugal's public debt during the war of liberation against the former Habsburg ruler. Using novel datasets on long‐ and short‐term debt and nominal interest rates, this study provides evidence that the sovereign borrower used debt credibility to build a pact of regime in a revolutionary context with implications for financing the war. The Portuguese kings followed an implicit budget balance rule as a reputational scheme, which made Portugal an exceptional case of military success with a low debt‐to‐GDP ratio and low interest rates. These conclusions contribute to the literature in various attributes of war finance, debt management, and state‐making by showing that default avoidance could be as important to military success as fiscal capacity.
A case of malignant transformation of equine immune‐mediated keratitis to B‐cell lymphoma
Authors: Lisankis Anthony P. , Beavis Brittany B. , Weigt Anne K. , Nunnery Catherine 
Published: November 2022

SummaryA 13‐year‐old Thoroughbred/Oldenburg gelding presented as a tertiary referral for a 6‐year history of immune‐mediated keratitis (IMMK) after the development of corneal and limbal nodules in the presence of medical therapy. A keratectomy extending to the limbus was performed to remove the affected cornea and limbal nodules. Two corneal‐limbal samples were submitted for histopathology and initially diagnosed as marked lympho‐histiocytic stromal keratitis with epithelial hyperplasia, keratinisation and keratinocyte apoptosis consistent with IMMK. Additional testing with immunohistochemical staining for CD3, CD20, PAX5 and IBA1 revealed large, atypical B‐lymphocytes on a background of histiocytes and mature T‐lymphocytes, with concern for T‐cell‐rich large B‐cell lymphoma. PCR antigen receptor rearrangement clonality testing was also performed and was diagnostic for large B‐cell lymphoma. Polymerase chain reaction (PCR) for equine herpesvirus 5 (EHV‐5) was positive. Following keratectomy, a short course of neomycin/polymyxin B/dexamethasone ophthalmic ointment was prescribed; no other treatment nor adjunct therapy was performed. Eighteen months post‐operation, the case remains visual and comfortable on no medications.
Family and consumer sciences graduate research productivity in 2021
Authors: Wilmarth Melissa J. , Milstead Morgan 
Published: November 2022

AbstractGraduate student degree completion and research has been collected annually since 1992. This year’s data collection began with an e‐mail invitation to 593 institutional contacts at 165 U.S. universities and colleges in June 2022. This e‐mail requested information on family and consumer sciences graduate degrees and research completed in 2021. A total of 150 master’s theses, 122 doctoral dissertations, and 664 non‐thesis master’s degrees were conferred in 2021 from family and consumer sciences programs. This review reports not only degree completion, but also highlights the topical trends of theses and dissertations completed within family and consumer sciences programs.
Participation of fibroblast growth factor‐1 and interleukin‐10 in connective tissue repair following subcutaneous implantation of bioceramic materials in rats
Authors: Delfino Mateus Machado , Jampani José Leandro de Abreu , Lopes Camila Soares , Guerreiro‐Tanomaru Juliane Maria , Tanomaru‐Filho Mário , Sasso‐Cerri Estela , Cerri Paulo Sérgio 
Published: November 2022

AbstractAimTo evaluate whether the bioceramic materials Bio‐C Pulpo (Bio‐C, Angelus) and mineral trioxide aggregate (MTA) Repair HP (MTA‐HP, Angelus) induce fibroblast proliferation and release of interleukin‐10 (IL‐10), an anti‐inflammatory cytokine, stimulating connective tissue remodelling. The tissue response of Bio‐C and MTA‐HP was compared with the White MTA (WMTA; Angelus) since studies have demonstrated that WMTA induces tissue repair.MethodologyBio‐C, MTA‐HP and WMTA were inserted into polyethylene tubes and implanted in the subcutaneous tissue of Holtzman rats for 7, 15, 30 and 60 days. As a control group (CG), empty tubes were implanted subcutaneously. The number of fibroblasts (FB), Ki‐67‐, fibroblast growth factor‐1‐ (FGF‐1) and IL‐10‐immunolabelled cells and collagen content in the capsules was obtained. The data were subjected to two‐way anova followed by Tukey's test (p ≤ .05).ResultsAt 7 days, significant differences in the number of FB were not detected amongst Bio‐C, MTA‐HP and WMTA groups (p ˃ .05). The capsules of all groups exhibited a significant increase in the number of FB and content of collagen over time. From 7 to 60 days, a significant reduction in the number of FGF‐1‐ and Ki‐67‐immunolabelled cells was seen in the capsules of all specimens. In all periods, no significant difference in the number of FGF‐1‐immunolabelled cells was detected between Bio‐C and CG specimens. At 60 days, significant differences in the immunoexpression of FGF‐1 were not observed amongst the groups. At 7 and 15 days, the highest immunoexpression for Ki‐67 was present in Bio‐C specimens whilst, after 30 and 60 days, no significant difference was observed amongst the bioceramic materials. At 7 days, few IL‐10 immunolabelled cells were present in the capsules of all specimens whereas, at 60 days, a significant increase in the IL‐10‐immunostaining was present in all groups. At 60 days, the Bio‐C, MTA‐HP and WMTA groups showed a greater number of IL‐10‐immunolabelled cells than in the CG specimens (p < .0001).ConclusionsBio‐C, MTA‐HP and WMTA stimulate fibroblast proliferation, leading to the formation of collagen‐rich capsules. FGF‐1 and IL‐10 may mediate the remodelling of capsules around Bio‐C, MTA‐HP and WMTA bioceramic materials.
Evaluation of the WBC‐differential flagging performances and accuracy of the HORIBA Yumizen H2500 cell counter in oncology patients from a single institution
Authors: Durrieu Françoise 
Published: November 2022

AbstractIntroductionThe Yumizen H2500 (YH2500) cell analyser was compared to the Pentra DX Nexus (both from HORIBA Medical) to evaluate the efficiency of the new YH2500 technology for the WBC‐differential in samples from oncology patients with WBC‐diff abnormalities.Material and MethodsThe 220 samples with slide review criteria on the Nexus were systematically analysed on the YH2500. The WBC‐diff was compared to flow cytometry (FCM). The 100 additional samples with Monocyte/Neutrophil separation flags were evaluated. The flagging performance and the accuracy of the WBC‐diff were analysed.ResultsThe YH2500 generated fewer flags than the Nexus (47.73% vs 66.64%; p < .0001), except for Monocyte/Neutrophil flag (15.5% vs. 2.7%; p < 0.0001). Overall performances were higher on the YH2500 (Sensitivity, 100 vs. 89.6%; Specificity 81.0% vs. 50.9%; PPV, 74.3% vs. 62.9%; NPV, 100% vs. 69.5%; Efficiency, 87.7% vs. 69.5%). YH2500 showed a statistically significant better correlation with FCM for the 6‐part differential than the Nexus. On 134 samples with Monocyte/Neutrophil separation flag, it was shown that, in samples with less than 10% monocytes on the YH2500, the results of monocyte and neutrophil counts were comparable to the manual count, and that these samples did not need a slide review if no other main criterion for review was present.ConclusionThe YH2500 demonstrated better performance characteristics than the previous cell counter, the Nexus. Its implementation in our laboratory routine work significantly improved the practice workflow, decreased the number of manual cell counts and increased the pertinence of slide review and reporting of the microscopic count.
Mast cells impair melanoma cell homing and metastasis by inhibiting HMGA1 secretion
Authors: Benito‐Martin Alberto , Nogués Laura , Hergueta‐Redondo Marta , Castellano‐Sanz Elena , Garvin Eduardo , Cioffi Michele , Sola‐Castrillo Paloma , Buehring Weston , Ximénez‐Embún Pilar , Muñoz Javier , Matei Irina , Villanueva Josep , Peinado Héctor 
Published: November 2022

ABSTRACTMetastatic disease is the major cause of death from cancer. From the primary tumour, cells remotely prepare the environment of the future metastatic sites by secreted factors and extracellular vesicles. During this process, known as pre‐metastatic niche formation, immune cells play a crucial role. Mast cells are haematopoietic bone marrow‐derived innate immune cells whose function in lung immune response to invading tumours remains to be defined. We found reduced melanoma lung metastasis in mast cell‐deficient mouse models (Wsh and MCTP5‐Cre‐RDTR), supporting a pro‐metastatic role for mast cells in vivo. However, due to evidence pointing to their antitumorigenic role, we studied the impact of mast cells in melanoma cell function in vitro. Surprisingly, in vitro co‐culture of bone‐marrow‐derived mast cells with melanoma cells showed that they have an intrinsic anti‐metastatic activity. Mass spectrometry analysis of melanoma‐mast cell co‐cultures secretome showed that HMGA1 secretion by melanoma cells was significantly impaired. Consistently, HMGA1 knockdown in B16‐F10 cells reduced their metastatic capacity in vivo. Importantly, analysis of HMGA1 expression in human melanoma tumours showed that metastatic tumours with high HMGA1 expression are associated with reduced overall and disease‐free survival. Moreover, we show that HMGA1 is reduced in the nuclei and enriched in the cytoplasm of melanoma metastatic lesions when compared to primary tumours. These data suggest that high HMGA1 expression and secretion from melanoma cells promote metastatic behaviour. Targeting HMGA1 expression intrinsically or extrinsically by mast cells actions reduce melanoma metastasis. Our results pave the way to the use of HMGA1 as anti‐metastatic target in melanoma as previously suggested in other cancer types.
Fused silica with sub‐angstrom roughness and minimal surface defects polished by ultrafine nano‐CeO2
Authors: Wu Zehua , Zhang Zhixin , Jia Yong , Teng Fei , Li Gang , Wang Feng , Jin Yuqi 
Published: November 2022

AbstractSurface quality of fused silica, particularly surface defect and surface roughness, is a key factor affecting the performance of high‐power laser and short‐wave optical instrument, and so on. Herein, the super smooth surface of fused silica with roughness of sub‐angstrom level and exceedingly few submicron defects was achieved by using ultrafine nano‐CeO2 with primary particle size less than 4 nm, low secondary particle agglomeration strength, and high Ce3+ concentration. Furthermore, CeO2 involve in polishing process in the form of primary particle was certified by experiment. Moreover, the cause for the generation of submicron defects on fused silica surface was investigated for the first time from the perspective of secondary particle agglomeration strength of CeO2. The concentration of Ce3+ in CeO2 was characterized by the redshift of the band‐gap energy, and the analysis of material removal rate (MRR) and contact angle of polished fused silica shows that Ce3+ enhances MRR through increasing the silanol group on fused silica.
Contact force catheter ablation for the treatment of persistent atrial fibrillation: Results from the PERSIST‐END study
Authors: Lo Monica , Nair Devi , Mansour Moussa , Calkins Hugh , Reddy Vivek Y. , Colley B. Judson , Tanaka‐Esposito Christine , Sundaram Sri , DeLurgio David B. , Sanders Prashanthan , Khatib Sammy , Bernard Michael , Olson Nicholas , Gibson Douglas , Miller Amber , Li Jingyun , Natale Andrea 
Published: November 2022

AbstractIntroductionUse of a novel magnetic sensor enabled optical contact force ablation catheter has been established to be safe and effective for treatment of symptomatic drug‐refractory paroxysmal atrial fibrillation (AF) but has yet to be demonstrated in the persistent AF (PersAF) population.MethodsPERSIST‐END was a multicenter, prospective, nonrandomized, investigational study designed to demonstrate the safety and effectiveness of TactiCath™ Ablation Catheter, Sensor Enabled™(SE) (TactiCath SE) for use in the treatment of subjects with documented PersAF refractory or intolerant to at least one Class I/III AAD. The ablation strategy included pulmonary vein isolation and additional targets at physician discretion. Follow‐up through 15‐months, including a 3‐month blanking period and 3‐month therapy consolidation period, was performed with cardiac event and Holter monitoring. Primary safety, primary effectiveness, clinical success, and quality of life (QOL) endpoints were analyzed.ResultsOf 224 subjects enrolled at 21 investigational sites in the United States and Australia, 223 underwent ablation with the investigational catheter. The primary safety event rate was 3.1% (seven events in seven subjects). The Kaplan–Meier estimate of freedom from AF/atrial flutter/atrial tachycardia recurrence at 15‐months was 61.6% and clinical success at 15 months was 89.8%. Subject QOL significantly improved following ablation as assessed via AFEQT (31.6 point increase, p < .0001) and EQ‐5D‐5L (10.7 point increase, p < .0001) and was met with a 53% reduction in all cause cardiovascular healthcare utilization.ConclusionThe sensor‐enabled force‐sensing catheter is safe and effective for the treatment of drug refractory recurrent symptomatic PersAF, reducing arrhythmia recurrence while improving QOL and healthcare utilization.
Resisting agrarian neoliberalism and authoritarianism: Struggles towards a progressive rural future in Mozambique
Authors: Monjane Boaventura 
Published: November 2022

AbstractAfter nearly two and a half decades with a Land Law widely considered progressive, Mozambique is preparing to revise its legal framework for land. Land activists accuse the government of pursuing an authoritarian approach, excluding civil society participation, and falsifying public consultations. The revision would mark a major shift in Mozambique's land policy towards an even more neoliberal framework to allow the transfer of individual land titles. This turning point is a crucial moment for popular movements to mobilize against the consolidation of agrarian neoliberalism and fight for pro‐poor land policy that benefits small‐scale food producers and rural communities at large. While recognizing different rural and agrarian class formations and interests in Mozambique, I argue that embryonic forms of a cross‐class alliance are becoming apparent. As deagrarianization proceeds, the National Union of Peasants (UNAC) plays a key role in mobilizing the rural poor — petty commodity producers, farm workers, fishermen, small agrarian capitalists, and agrarian civil society at large — using left‐wing populism to oppose agrarian neoliberalism, which takes authoritarian forms.
Perspectives on interpersonal touch are related to subjective sleep quality
Authors: Dueren Anna Lena , Bowling Natalie C. , Vafeiadou Aikaterini , Madrid‐Valero Juan J. , Hammond Claudia , Gregory Alice M. , Banissy Michael J. 
Published: November 2022

SummaryAffective touch has been reported for its calming effects; however, it is less clear whether touch is associated with sleep. Here, the relationship between different touch variables and self‐reported sleep indicators was investigated. Data were extracted from the Touch Test, a cross‐sectional survey conducted in 2020. Data from a sample of 15,049 healthy adults from the UK (mean age = 56.13, SD = 13.8; 75.4% female) were analysed. Participants were asked to attribute positive, negative, or no effects on sleep to hugs, strokes, massages, intimate touch, and sleep onset with and without touch. The time since last intentional touch, touch amount satisfaction, and childhood bed routine with hugs and kisses were assessed. Sleep quality, duration, latency, wake after sleep onset and diurnal preference were measured. Data were analysed using chi‐square tests and logistic regressions. Affective touch before sleep was perceived to have positive effects on sleep. Touch recency emerged as a significant predictor for some sleep variables, with a longer timespan since the last intentional touch relating to improved sleep quality, longer sleep duration, and shorter and fewer instances of waking up after sleep onset in some participants. Experiencing too much touch was related to lower sleep quality and higher instances of waking up after sleep onset. These findings highlight the importance of interpersonal touch for subjective sleep quality.
Symptom dynamics among nightmare sufferers: An intensive longitudinal study
Authors: Dumser Britta , Werner Gabriela G. , Ehring Thomas , Takano Keisuke 
Published: November 2022

SummaryNightmares are considerably prevalent in the general population and are known to be closely associated with a variety of mental disorders. However, not much is known about the immediate antecedents and consequences of nightmares. Therefore, we used intensive longitudinal assessments to investigate the night‐to‐night within‐person associations between nightmares on the one hand and fear of sleep, somatic as well as cognitive pre‐sleep arousal, and sleep quality on the other hand. Young women with regular nightmares (n = 16) maintained a sleep diary for around 30 days; upon awaking, the participants reported on nightmares and sleep quality during the past night as well as the pre‐sleep levels of arousal and fear of sleep (which resulted in 461 observations). Participants also wore an actigraph, which provided objective sleep parameters. Multilevel modeling showed that higher levels of fear of sleep and lower subjective sleep quality were significantly associated with higher levels of nightmare distress. Furthermore, we found individual differences in the strength of these associations, which implies that factors proximate to nightmares may vary across individuals. Pre‐sleep arousal, however, did not show expected within‐person associations with nightmares or fear of sleep. These findings highlight the crucial role of fear of sleep in the etiology of nightmares and sleep disturbances, while pointing to the importance of pursuing individual, personalised models that explain heterogeneity in the process of triggering nightmares.
Classification and survival prediction in early‐stage cirrhosis by gene expression profiling
Authors: Wang Qingliang , Li Xiaojie , Chen Yaqiong , Gong Jiao , Hu Bo 
Published: November 2022

AbstractLiver cirrhosis has been increasingly diagnosed at an early stage owing to the non‐invasive diagnostic techniques. However, it is difficult to identify patients at high risk of disease progression. Screening cirrhotic patients with poor prognosis who are most in need of surveillance is still challenging. Gene expression data GSE15654 and GSE14520 were downloaded for performing unsupervised clustering analysis. The prognostic differences between the different clusters were explored by Cox regression. Integrative analysis of gene expression signature, immune cell enrichments and clinical characterization was performed for different clusters. Two distinctive subclasses were identified in HCV‐related GSE15654, and Kaplan–Meier analysis indicated that subtype 2 had lower survival rates than subtype 1 (p = 0.0399). Further analysis revealed subtype 2 had a higher density of follicular T helper cells, resting natural killer cells and M0, M2 macrophages while subtype 1 with a higher fraction of naive B cells, memory B cells, resting memory CD 4 T cells, activated natural killer cells and monocytes. 226 differentially expressed genes were identified between the two subtypes, and Reactome analysis showed the mainly enriched pathways were biological oxidations and fatty acid metabolism. Five hub genes (AKT1, RPS16, CDC42, CCND1 and PCBP2) and three significant modules were extracted from the PPI network. The results were validated in HBV‐related GSE14520 cohort. We identified two subtypes of patients with different prognosis for hepatitis C‐related early‐stage liver cirrhosis. Bioinformatics analysis of the gene expression and immune cell profile may provide fresh insight into understanding the prognosis difference.
Relative adrenal insufficiency in the non‐critically ill patient with cirrhosis: A systematic review and meta‐analysis
Authors: Wentworth Brian J. , Schliep Matthew , Novicoff Wendy , Siragy Helmy M. , Geng Calvin X. , Henry Zachary H. 
Published: November 2022

AbstractBackground & AimsCharacterization of relative adrenal insufficiency (RAI) in cirrhosis is heterogeneous with regard to studied patient populations and diagnostic methodology. We aimed to describe the prevalence and prognostic importance of RAI in non‐critically ill patients with cirrhosis.MethodsA systematic review and meta‐analysis was performed using MeSH terms and Boolean operators to search five large databases (Ovid‐MEDLINE, ScienceDirect, Web of Science, Cochrane Library and ClinicalTrials.gov). The population of interest was patients with cirrhosis and without critical illness. The primary outcome was the pooled prevalence of RAI as defined by a peak total cortisol level <18 μg/dl, delta total cortisol <9 μg/dl or composite of the two thresholds in response either a standard‐dose or low‐dose short synacthen test. Odds ratios and standardized mean differences from random‐effects models estimated important clinical outcomes and patient characteristics by adrenal functional status.ResultsTwenty‐two studies were included in final analysis, comprising 1991 patients with cirrhosis. The pooled prevalence of RAI was 37% (95% CI 33–42%). The prevalence of RAI varied by Child–Pugh classification, type of stimulation test used, specific diagnostic threshold and by severity of illness. Ninety‐day mortality was significantly higher in patients with RAI (OR 2.88, 95% CI 1.69–4.92, I2 = 15%, p < 0.001).ConclusionsRelative adrenal insufficiency is highly prevalent in non‐critically ill patients with cirrhosis and associated with increased mortality. Despite the proposed multifactorial pathogenesis, no studies to date have investigated therapeutic interventions in this specific population.