bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Arabidopsis Framework Model version 2 predicts the organism-level effects of circadian clock gene mis-regulation
Authors: Chew Yin Hoon , Seaton Daniel D , Mengin Virginie , Flis Anna , Mugford Sam T , George Gavin M , Moulin Michael , Hume Alastair , Zeeman Samuel C , Fitzpatrick Teresa B , Smith Alison M , Stitt Mark , Millar Andrew J 
Published: May 2022

Abstract
Predicting a multicellular organism’s phenotype quantitatively from its genotype is challenging, as genetic effects must propagate across scales. Circadian clocks are intracellular regulators that control temporal gene expression patterns and hence metabolism, physiology and behaviour. Here we explain and predict canonical phenotypes of circadian timing in a multicellular, model organism. We used diverse metabolic and physiological data to combine and extend mathematical models of rhythmic gene expression, photoperiod-dependent flowering, elongation growth and starch metabolism within a Framework Model for the vegetative growth of Arabidopsis thaliana, sharing the model and data files in a structured, public resource. The calibrated model predicted the effect of altered circadian timing upon each particular phenotype in clock-mutant plants under standard laboratory conditions. Altered night-time metabolism of stored starch accounted for most of the decrease in whole-plant biomass, as previously proposed. Mobilization of a secondary store of malate and fumarate was also mis-regulated, accounting for any remaining biomass defect. The three candidate mechanisms tested did not explain this organic acid accumulation. Our results link genotype through specific processes to higher-level phenotypes, formalizing our understanding of a subtle, pleiotropic syndrome at the whole-organism level, and validating the systems approach to understand complex traits starting from intracellular circuits.
Oridonin suppresses particulate-induced NLRP3-independent IL-1α release to prevent crystallopathy in the lung
Authors: Ikoma Kenta , Takahama Michihiro , Kimishima Atsushi , Pan Yixi , Taura Manabu , Nakayama Akiyoshi , Arai Masayoshi , Takemura Naoki , Saitoh Tatsuya 
Published: May 2022

AbstractThe human body is exposed to various particulates of industrial, environmental, or endogenous origin. Invading or intrinsic particulates can induce inflammation by aberrantly activating the immune system, thereby causing crystallopathies. When immune cells such as macrophages phagocytose the particulates, their phagolysosomal membranes undergo mechanical damage, eventually leading to pyroptotic cell death accompanied by the release of inflammatory cytokines, including interleukin (IL)-1α and IL-1β. The nod-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is responsible for particulate-induced IL-1β release and is therefore regarded as a potential therapeutic target for inflammation-mediated crystallopathies. However, IL-1α is released after particulate stimulation in an NLRP3 inflammasome-independent manner and plays a critical role in disease development. Therefore, drugs that exert potent anti-inflammatory effects by comprehensively suppressing particulate-induced responses, including IL-1β release and IL-1α release, should be developed. Here, we found that oridonin, a diterpenoid isolated from Isodon japonicus HARA, strongly suppressed particulate-induced cell death, accompanied by the release of IL-1α and IL-1β in mouse and human macrophages. Oridonin reduced particulate-induced phagolysosomal membrane damage in macrophages without affecting phagocytosis of particulates. Furthermore, oridonin treatment markedly suppressed the symptoms of silica particle-induced pneumonia, which was attributed to the release of IL-1α independently of NLRP3. Thus, oridonin is a potential lead compound for developing effective therapeutics for crystallopathies attributed to NLRP3-dependent as well as NLRP3-independent inflammation.
Sustaining Capitalism and Democracy: Lessons from Global Competition Policy
Authors: Allen Michael O , Scheve Kenneth 
Published: May 2022

AbstractCompetition policy has been a central forum for contesting the uneasy relationship between capitalism and democracy since the late nineteenth century. From the earliest policy debates, concerns that robust competition policies aimed at limiting economic concentration would disadvantage domestic producers featured prominently. This dynamic creates an international cooperation problem over competition policy that has intensified with the dramatic increases in globalization over the last several decades. Understanding the causes and consequences of this cooperation problem is central to understanding global governance of the world economy, including the ability of democratic states to manage global capitalism in a manner that sustains democracy. This essay frames the challenge that global competition policy has posed over the last hundred years and argues that democratic major powers have played a critical role in shaping the limited cooperation that has been achieved. A research agenda that develops integrated theories of domestic and international competition policymaking as well as a multi-method empirical agenda for describing and explaining policy outcomes and their consequences for economic inequality, growth, and democracy is an essential task for international relations scholars.
Rediscovering Epistemic Coalitions Twenty Years Later: Using the International Olympic Committee to Build toward A Literature on Epistemic Institutionalism
Authors: Espino Giuliano 
Published: May 2022

AbstractThis article aims to help the literature on global governance and international professionalism move beyond a myopic focus on epistemic communities by unpacking the underutilized term epistemic coalition. Epistemic coalitions are an international nonstate actor that employ a broadened notion of expertise to incorporate business and political advocates alongside technical experts. I differentiate epistemic coalitions from epistemic communities by synthesizing the international relations, organizational theory, and public policy literatures. These organizations differ in the breadth of their policy agendas, dynamics of internal cohesion, and capabilities to engage in global policy transfer. However, I note that it is still unclear how epistemic coalitions coordinate member action along different professions and what are the conditions under which epistemic coalitions’ membership balances will be viewed by national elites as professionally legitimate. I then examine the role of the International Olympic Committee in US steroid policy to induce two preliminary hypotheses that can potentially address these gaps. First, epistemic coalitions use a foundational narrative to coordinate action among professions. Second, perceptions of equitable membership balance stem from an ideational congruence between national and international elites. I conclude with how these hypotheses can be tested in the future.
Trends in human intestinal carriage of ESBL- and carbapenemase-producing Enterobacterales among food handlers in Tunisia: emergence of C1-M27-ST131 subclades, blaOXA-48 and blaNDM
Authors: Sallem Nesrine , Hammami Adnene , Mnif Basma 
Published: May 2022

Abstract

Objectives
To determine the prevalence of community intestinal carriage of ESBL-producing Enterobacterales (ESBL-E), acquired-AmpC-producing Enterobacterales (aAmpC-E) and carbapenemase-producing Enterobacterales (CPE) in Tunisia.

Methods
From November 2012 to September 2017, stool samples from food handlers in Sfax, Tunisia, were screened for ESBL-E, AmpC-E and CPE using antibiotic-containing media. The genes encoding these β-lactamases were characterized by PCR, sequencing, and transfer assays. ST131 clonal groups were detected by PCR and characterized for antibiotic resistance, virulence genes and PFGE patterns.

Results
Of 2135 participants, ESBL-E, aAmpC-E, and CPE carriage were detected in 419 (19.63%), 35 (1.63%) and 7 (0.33%) participants, respectively. CTX-M-15 (60%), CTX-M-1 (16.8%) and CTX-M-27 (12.8%) were the most common ESBL determinants. The ESBL-E carriage was significantly higher in summer (33%) and autumn (25.7%) than in winter (12.1%) and spring (11.4%). ST131 was detected in 50 (13.2%) of the 378 ESBL-producing Escherichia coli isolates; most of them (35; 70%) belonged to subclade C1 (cluster C1-M27: 23 isolates, 46%; cluster C1-non-M27: 12 isolates, 24%) followed by those belonging to subclade C2 (11; 22%). Although subclade C2 isolates, all harbouring blaCTX-M-15, had the highest resistance rates and virulence factor and addiction system scores, the subclade C1 isolates, mainly harbouring blaCTX-M-27 (94%), were predominant since 2015. The most frequently detected carbapenemase-encoding gene was blaOXA-48-like (85%) and acquired AmpC-encoding genes were blaDHA-1 (54%) and blaCMY-2 (46%).

Conclusions
This is the first large Tunisian study to reveal a high faecal ESBL carriage rate, a low CPE carriage rate, and the predominance of CTX-M-27-producing subclade C1 among faecal ESBL-ST131 isolates in the Tunisian community.
Critical loss in market definition: methods and court decisions
Authors: Coate Malcolm B , Ulrick Shawn W , Yun John M 
Published: May 2022

Abstract
Critical loss analysis is an empirical tool used to define relevant markets in antitrust law. The existence of two different critical loss methodologies, however, complicates its application. Harris and Simons introduced the first approach, which focused on evaluating the market-level effect of a small, but significant and non-transitory increase in price (‘SSNIP’). Later, O’Brien and Wickelgren, along with Katz and Shapiro, introduced a firm-level approach to critical loss to derive a test that applies mathematical models of demand systems, foundationally based on a single-firm SSNIP, to proxy for a market-level price increase. A critical loss controversy evolved as the two tests can, but do not necessarily, generate different relevant markets. This article examines the choice between the two methods, reviews recent court decisions, and guides practitioners and courts as to when each approach makes the most sense.
Relationship between electronic health records strategy and user satisfaction: a longitudinal study using clinicians’ online reviews
Authors: Srivastava Ankita , Ayyalasomayajula Surya , Bao Chenzhang , Ayabakan Sezgin , Delen Dursun 
Published: May 2022

Abstract

Objective
We investigated how the electronic health records (EHRs) strategies concerning EHR sourcing and vendor switching impact user satisfaction over time.

Materials and Methods
This study used a novel longitudinal dataset created by scraping clinicians’ Glassdoor.com reviews on 109 US health systems from 2012 to 2017 and combining it with the Healthcare Information and Management Systems Society (HIMSS) database. We performed sentiment analysis of clinician reviews to construct our main dependent variable, user satisfaction. Our main independent variables, EHR single sourcing and vendor switching, were constructed using the HIMSS database.

Results
Our fixed effects model showed that as health systems gain more experience with EHR, a single vendor sourcing strategy was associated with higher user satisfaction. Further, there was no significant impact of vendor switching on user satisfaction.

Conclusion
This work adds to the current understanding of EHR-driven clinician burnout using a novel longitudinal dataset. We show how organizational-level EHR strategy can impact user satisfaction and that providers and EHR vendors can mine clinician reviews online to understand their evolving needs and sentiments.
Evaluation of an online text simplification editor using manual and automated metrics for perceived and actual text difficulty
Authors: Leroy Gondy , Kauchak David , Haeger Diane , Spegman Douglas 
Published: May 2022

Abstract

Objective
Simplifying healthcare text to improve understanding is difficult but critical to improve health literacy. Unfortunately, few tools exist that have been shown objectively to improve text and understanding. We developed an online editor that integrates simplification algorithms that suggest concrete simplifications, all of which have been shown individually to affect text difficulty.

Materials and Methods
The editor was used by a health educator at a local community health center to simplify 4 texts. A controlled experiment was conducted with community center members to measure perceived and actual difficulty of the original and simplified texts. Perceived difficulty was measured using a Likert scale; actual difficulty with multiple-choice questions and with free recall of information evaluated by the educator and 2 sets of automated metrics.

Results
The results show that perceived difficulty improved with simplification. Several multiple-choice questions, measuring actual difficulty, were answered more correctly with the simplified text. Free recall of information showed no improvement based on the educator evaluation but was better for simplified texts when measured with automated metrics. Two follow-up analyses showed that self-reported education level and the amount of English spoken at home positively correlated with question accuracy for original texts and the effect disappears with simplified text.

Discussion
Simplifying text is difficult and the results are subtle. However, using a variety of different metrics helps quantify the effects of changes.

Conclusion
Text simplification can be supported by algorithmic tools. Without requiring tool training or linguistic knowledge, our simplification editor helped simplify healthcare related texts.
Optimization of Solid-Phase Extraction of a Degradation Product of Novichok (A234) and Its Application to Environmental Samples
Authors: Lee Jin Young , Shin Ji Young , Kim Hyun Suk 
Published: May 2022

AbstractThere have been no detailed investigations regarding solid-phase-extraction (SPE) optimization and screening for the degradation products of ethyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A234) in various environmental samples. Therefore, as a first step in the selective SPE of the degradation products of A234, we optimized the SPE adsorption and extraction parameters for the A234 degradation product ethylhydrogen (1-(diethylamino)ethylidene)phosphoramidate (cpd 1). Among various SPE cartridges, the Si cartridge (500 mg, 3 mL) selectively extracted cpd 1 using an elution volume of 4 mL of 25% H2O in acetonitrile, which eliminated most interference without cpd 1 loss during loading and washing. In addition, the sorbent capacity is also critical in the adsorption of cpd 1. The Si cartridge (500 mg, 3 mL) retained cpd 1 in the concentration range 1–10 µg/mL. The linearity of detector response of cpd 1 in deionized H2O was studied in the range of 1.0–100 ng/mL and showed good linearity with γ2 ranging from 0.9979 to 0.996. The limits of detection for cpd 1 are 10 ng/mL in the product-scan mode and 100 ng/mL in the full-scan mode. Also, after we optimized the SPE method, we validated the precision and accuracy of the Si-cartridge extraction method in real soil samples with diverse concentrations. The precision ranged from 2.5% to 5.3%. This newly developed SPE is applicable to the analysis of a degradation product of Novichok A234 in various environmental matrices, such as water, soil and sand, in the Organization for the Prohibition of Chemical Weapons (OPCW) proficiency test and unknown samples collected from suspected sites.
Polyamines produced by an extreme thermophile are essential for cell growth at high temperature
Authors: Sakamoto Akihiko , Tamakoshi Masatada , Moriya Toshiyuki , Oshima Tairo , Takao Koichi , Sugita Yoshiaki , Furuchi Takemitsu , Niitsu Masaru , Uemura Takeshi , Igarashi Kazuei , Kashiwagi Keiko , Terui Yusuke 
Published: May 2022

Abstract
An extreme thermophile, Thermus thermophilus grows at an optimum temperature of around 70°C and produces 16 different polyamines including long-chain and branched-chain polyamines. We found that the composition of polyamines in the thermophile cells changes with culture temperature. Long-chain and branched-chain polyamines (unusual polyamines) were increased in the cells grown at high temperature such as 80°C, but they were minor components in the cells grown at relatively lower temperature such as 60°C. The effects of polyamines on cell growth were studied using T. thermophilus HB8 ΔspeA deficient in arginine decarboxylase. Cell growth of this mutant strain was significantly decreased at 70°C. This mutant strain cannot produce polyamines and grows poorly at 75°C. It was also determined whether polyamines are directly involved in protecting DNA from DNA double-strand breaks (DSBs) induced by heat. Polyamines protected DNA against double-strand breaks. Therefore, polyamines play essential roles in cell growth at extremely high temperature through maintaining a functional conformation of DNA against DSBs and depurination.
Radiologic-Pathologic Correlation of Fibroepithelial Lesions: A Pictorial Review
Authors: Zhang Meng , Compton Lindsay , Hwang Helena , Hayes Jody C 
Published: May 2022

Abstract
Fibroepithelial lesions (FEL) of the breast encompass a spectrum of masses ranging from benign to malignant. Although these lesions are on the same biologic spectrum, differences in their clinical behaviors necessitate different management approaches. While imaging features are nonspecific, small size (less than 3 cm), oval shape, circumscribed margins, growth in diameter less than 20% in six months, and homogeneous echotexture on US favor fibroadenoma (FA). Conversely, larger size (3 cm or larger), rapid growth, irregular shape, noncircumscribed margins, and heterogeneous echotexture suggest possible phyllodes tumor (PT). Histopathologically, increased stromal cellularity, stromal atypia, and mitotic activity characterize PT, while FA typically lack these features. In this review, we summarize the imaging and pathology characteristics of nonmalignant FEL, including simple, juvenile, and complex FA, and benign and borderline PT and highlight the collaborative role of radiologists and pathologists in informing diagnosis and clinical management.
Hiding in Plain Sight: An Abundant and Widespread North American Horse Fly (Diptera: Tabanidae) in the Tabanus sulcifrons Group, Tabanus variegatus Fabricius, Redescribed
Authors: Mullens Bradley A , Trout Fryxell Rebecca , Masonick Paul K , Yanega Douglas A , Davis Travis M 
Published: May 2022

Abstract
Tabanus variegatus F. 1805 has been called by the name Tabanus sulcifronsMacquart 1855 for over 80 yr; T. variegatus is one of the most common large horse flies attacking livestock in much of the southeastern U.S. Morphological, ecological, and molecular evidence indicates that T. variegatus is a distinct species, and we redescribe the female and describe the male. The Fabricius holotype, heavily damaged after over nearly 220 yr, is nevertheless taxonomically sound. Morphology (size, color, palp shape, and r5 wing cell shape) can usually distinguish T. variegatus from T. sulcifrons, but some specimens remain difficult to separate, especially in and west of the Mississippi River Valley. Using geometric morphometric analyses of the wing vein arrangement and palp shape the two species are significantly different. The wings of T. variegatus females also have more microsetae and sometimes a “frosty” appearance. Where they are common and sympatric, as in eastern Tennessee, they are temporally separated such that T. variegatus flies later (August-October) than T. sulcifrons (June-August), minimizing opportunity for gene flow. Museum specimens allow the approximate range of T. variegatus to be compared with that of T. sulcifrons s.l.; T. variegatus is particularly abundant from the coast of the Carolinas and Georgia east to central Tennessee and south to about central Alabama. DNA evidence (COI gene) recovers T. variegatus and T. sulcifrons s.s. in separate clades. Further studies on the T. sulcifrons complex are needed to fully resolve the range of both species, assess the degree of genetic substructuring, and examine relationships with other members of the T. sulcifrons complex.
Effect of Larval Diets on House Fly (Diptera: Muscidae) Production and Host Suitability for a Pupal House Fly Parasitoid (Spalangia gemina) (Hymenoptera: Pteromalidae)
Authors: Ardburi Waranya , Tangkawanit Ubon 
Published: May 2022

AbstractA larval medium for house fly (Musca domestica L.) suitable for house fly parasitoid production was developed from locally available and inexpensive food ingredients. Biological parameters and life table parameters were estimated for house flies treated with five different diets. It was found that percentage survival of 1st–3rd instar larvae of house fly, percentage of pupation, percentage of hatching adults, growth index, and life table parameters (net reproductive rate [R0] the cohort generation time [T], intrinsic rate of increase [r], and finite rate of increase [λ]) were significantly highest on larvae produced on diet 5 (composed of rice barn and chicken feed). Diet composition and performance of house fly larvae in larval medium are discussed, based on the results. The results of Spalangia gemina Bouček (Hymenoptera, Pteromalidae) parasitization on house fly pupae revealed that the number of total parasitized pupa and number of parasitoids hatched from pupa reared with diet 5 (rice bran and chicken feed) were greater than with diet 1 (rice bran, powdered milk, dry yeast, fish meal, soybean meal, and chicken feed). Additionally, S. gemina offspring from diet 5 treatment had a higher proportion of females. Our results indicated that pupal size resulting from larval diet was an important factor for parasitization.
A functional platform for remote use of electron microscopes using web conferencing systems
Authors: Sugiura-Nakazato Makoto , Takase Hiroshi , Nakazato Takeru 
Published: May 2022

Abstract
We propose a useful system for remote sharing of transmission electron microscope (TEM) images by connecting three computers: a computer connected to a TEM, a computer distributing images and a computer receiving images. Then, we confirmed the performance of three web conferencing systems, Microsoft Teams, Zoom and Google Meet, to evaluate the usefulness of their remote use based on the clarity of images, smoothness of movement and time lag in images on each computer in the system. Display image of operation computer for TEM can be captured using the following two methods: a virtual camera of a video distribution software that provided a good reaction speed to transfer images and the screen sharing by conference system software that could share high-quality images.