bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green Synthesis of Silver Nanoparticles of Vernonia cinerea Leaf Extract
and their In vitro Cytotoxicity Activity against Neuroblastoma SHSY-5Y
Cell Lines, Antimicrobial and Antioxidant Studies
Authors: Palei Narahari N. , Krishnan S. Navaneetha , Jayaraman R. , Reddy S. Hemanth , Balaji Anna , Samanta Malay K. , Mohanta Bibhash C. 
Published: May 2022

Background:
Green syntheses of silver nanoparticles using plant extracts have potential anti-
cancer, antimicrobial, and antioxidant properties, among other aspects. The aim of the present study
was to synthesize silver nanoparticles (AgNPs) using Vernonia cinerea plant extract.

Methods:
The AgNPs were successfully prepared and characterized using UV-Vis Spectrophotometer,
particle size, Zeta potential, Transmission electron microscopy (TEM), Energy-dispersive x-ray analysis
(EDAX), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectrometry. The in
vitro cytotoxicity study was performed using neuroblastoma SHSY-5Y cell lines. Moreover, antimicrobial
and antioxidant activity studies were also performed for AgNPs.

Results:
The size of AgNPs determined through the dynamic light scattering (DLS) technique was 49.5
nm and the zeta potential was -36.8 mV. The synthesized AgNPs were checked using UV-Visible spectroscopy
at ƛmax 439 nm. The color was changed from green to dark brown, indicating the formation
of AgNPs. The TEM study revealed that the nanoparticles were spherical in shape. The XRD pattern of
AgNPs produced in this experiment was apparently crystalline. The results of FTIR study revealed that
the majority of the obtained peaks correspond to the polyphenols, triterpenoids, and alkaloids which
were abundant in the corresponding to the V. cinerea leaf extract and support to the formation of
AgNPs.
The cytotoxicity effect of the V. cinerea plant extract and biosynthesized AgNPs was found to be dosedependent.
From the results of antimicrobial studies, it was reported that the gram negative bacteria
were found to be more susceptible compared to the gram positive bacteria. Moreover, the results of antioxidant
study revealed that the AgNPs showed good antioxidant activity (77.21%) in comparison to
the V. cinerea plant extract (56.13%).

Conclusion:
Based on the results, it could be concluded that the green synthesized silver nanoparticles
showed promising anticancer, antioxidant, and anti-bacterial activities as compared to the plain V. cineria
plant extract.
Recent Patents on 3D Printing Technology in Artificial Bone Printing
Devices, Materials, and Related Applications
Authors: Li Zhaolong , Wang Qinghai 
Published: May 2022

Background:
3D printing is a kind of rapid prototyping technology. It is a digital model file-based technology that uses powdered metals, plastics and other bond able materials to construct objects by stacking them layer by layer. In recent years, the use of 3D printed artificial bones for repairing or re-placing damaged bones has been widely used. Therefore, the application of 3d printing technology in the field of orthopedics has attracted more and more attention.

Objective:
This paper introduces the research status of 3D printing technology in the field of orthope-dics, and discusses the future development direction.

Method:
Summarize various representative patents related to 3D printing bones all over the world.

Results:
By summarizing a large number of patents related to 3D printing bones, the research status of printing devices, printing materials, structure optimization and related applications of 3D printing bones are analyzed, and the related problems and development trends of 3D printing bones are dis-cussed.

Conclusion:
The combination of 3D printed bone materials and the optimization of the artificial bone unit structure will improve the mechanical properties of bone grafts and increase the success rate of transplantation. Bone repair aids and surgical guides will improve the success rate of orthopedic sur-gery and will be more widely used in the future. 3D printing technology is more and more widely used in the field of orthopedics, and there will be more research results in the future.
Phytochemistry and Pharmacological Aspects of Apigenin: A Review
Authors: Bhardawaj Hemlata , Vasudeva Neeru , Sharma Sunil 
Published: May 2022

Background:
A bioactive flavone, apigenin is plentifully present in common vegetables and fruits including spinach, parsley, celery stalks, peas, black olives, olive oil, grapefruit, kumquat, honey etc. It is a low-toxic flavonoid. Flavonoids are currently considered an important component in a huge range of cosmetic, pharmacological, and medical formulations. According to epidemiological research flavone-rich diets have been related to a lower risk of cancer notably, lung, skin, prostate, breast cancers and certain hematological disorders. It has been proposed as a preventive agent in other disorders such as oxidative stress, inflammation, cardiovascular and neurological issues. Apigenin-enriched medications are more effective for some chronic conditions, but if consumed regularly, they can harm animal and human health such as stomach discomfort, skin irritation, numbness and prolonged sedation etc. Apigenin's bioavailability limitations need the development of innovative carriers to increase bioavailability. Flavonoid’s data is gathered from the major public databank in order to emphasize apigenin's important role in the treatment and prevention of numerous illnesses, as well as to explore its health consequences.

Method:
The literature review of the apigenin was collected from various databases viz. Web of Science, PubMed, Google Scholar, Google and Science Direct etc.

Results:
The compiled data on the therapeutic efficacy of apigenin and its derivatives provides a platform for future researcher.

Conclusion:
The data revealed the pharmacological effects viz. anti-microbial, anti-cancer, anti-oxidant, anti-viral, anti-inflammatory, anti-diabetic, ischemic stroke and anti-arthritic activities of apigenin isolated by using different solvents and analytical methods that can be useful in the utilization for the development and formulation of herbal preparation by future researchers.
Validation of Gender Identification by “Alu” Insertion on Y Chromosome
for Forensic Mass Disaster
Authors: Israfil S.M. Hasan , Begum Rokeya , Sultana Gazi Nurun Nahar 
Published: May 2022

Background:
Gender determination of human samples is critical in forensic identification.
The most popular forensic kit AmpliFlSTR® is based on Short Tandem Repeat (STR), and
gender determination is performed on a single copy Amelogenin gene with X and Y homologous
chromosomes. This method is not sufficiently sensitive for degraded and a small number of samples
and requires a lengthy time. Furthermore, this method needs fragment analysis by a genetic
analyzer and an analytical kit for sample preparation, which is costly and not available for public
support in developing countries.

Aim:
Thus, it is urgent to have an alternative, quick, and cost-effective gender identification method
during mass disasters.

Objective:
We proposed to use an alternative PCR method with a single AluSTYa primer and compare
it with our designed primer to identify male and female DNA samples in mass disasters when
identification is crucial.

Method:
In the current research, we scanned 16 samples from the fire incidence area known as
Mohammadpur slum of Dhaka city in different time frames, where bodies were partially burnt.
Patients were admitted to the burn unit of Dhaka Medical College, Dhaka. Thirty-six unknown
samples from the healthy cohort were included from the Dhaka University campus for comparison.
Modified Alu primer was used in addition to known commercial AluSTYa primers.

Results:
This study revealed that our designed primer and commercial AluSTYa primer could identify
Alu insertion by a simple PCR method into a non-recombinant Y homologous region. The
results showed the high quality of DNA extracted from the burnt specimens and a 100% difference
in the number of bands in X and Y chromosomes by a simple, robust, and cost-effective PCR and
agarose gel electrophoresis method for gender identification.

Conclusion:
This method can be applied to mass disaster incidents where rapid gender identification
of unknown persons is urgent for forensic purposes.
Familiarity in Rural Life: Protocol for a Scoping Review and Concept Analysis
Authors: Swan Marilyn A , Gietzen Luke J , Hobbs Barbara B 
Published: May 2022

Background
Familiarity is a concept often used in literature but is not well defined or understood. As a key concept in rural nursing theory, the conceptual understanding of familiarity is currently incomplete. The findings from this scoping review will inform a concept analysis using Walker and Avant’s method and to identify and define the missing key components of familiarity.

Objective
The objective of this scoping review is to examine and analyze what is known in the existing literature about the concept of familiarity.

Methods
The Joanna Briggs Institute scoping review framework guided the identification of literature published from 2016 to 2022 on familiarity. Following the PRISMA-ScR (Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews) reporting standard, the familiarity scoping review is registered on Open Science Framework (registration digital object identifier: 10.17605/OSF.IO/ZB8VF). A total of 8 databases, including PubMed, CINAHL (Cumulative Index to Nursing and Allied Health Literature) Plus with full text, APA PsychInfo, Communication Source, EBSCO MegaFILE, Medline, Nursing & Allied Health Database, and ScienceDirect, will be searched for 22 search terms. Covidence software will be used to manage the scoping review with each citation independently reviewed by 2 research team members for eligibility. Eligibility will be determined using a 2-level process. Each title and abstract will be screened for eligibility; for citations deemed eligible, a full-text article review will be conducted. The scoping review is expected to locate a large body of literature, and eligibility criteria will be refined during the title and abstract screening process. In addition, reference list scanning will be performed to locate relevant literature.

Results
Familiarity data will be collected beginning October 2021 with anticipated completion in March 2022. Dissemination of findings will occur through scholarly presentations and in rural-focused and nursing publications in 2022 or 2023. The findings from this review will further the understanding of familiarity and how it affects rural life and nursing practice.

Conclusions
This review will support a full understanding and add clarity to the concept of familiarity as a component of rural life. These new insights will advance the understanding of how familiarity influences rural health care practice. The concept analysis will provide theoretical support for rural nursing theory and promote an understanding of the interrelationships of rural concepts.

International Registered Report Identifier (IRRID)
PRR1-10.2196/36930
Evaluation of Conflicts of Interest among Participants of the Japanese Nephrology Clinical Practice Guideline
Authors: Murayama Anju , Yamada Kohki , Yoshida Makoto , Kaneda Yudai , Saito Hiroaki , Sawano Toyoaki , Shrestha Sunil , Shrestha Rajeev , Tanimoto Tetsuya , Ozaki Akihiko 
Published: May 2022

Background and objectives
Rigorous and transparent management strategies for conflicts of interest and clinical practice guidelines with the best available evidence are necessary for the development of nephrology guidelines. However, there was no study assessing financial and nonfinancial conflicts of interest, quality of evidence underlying the Japanese guidelines for CKD, and conflict of interest policies for guideline development.

Design, setting, participants, & measurements
This cross-sectional study examined financial and nonfinancial conflicts of interest among all 142 authors of CKD guidelines issued by the Japanese Society of Nephrology using a personal payment database from all 92 major Japanese pharmaceutical companies between 2016 and 2019 and self-citations by guideline authors. Also, the quality of evidence and strength of recommendations underlying the guidelines and conflicts of interest policies of Japanese, US, and European nephrology societies were evaluated.

Results
Among 142 authors, 125 authors (88%) received $6,742,889 in personal payments from 56 pharmaceutical companies between 2016 and 2019. Four-year combined median payment per author was $8258 (interquartile range, $2230‒$51,617). The amounts of payments and proportion of guideline authors with payments remained stable during and after guideline development. The chairperson, vice chairperson, and group leaders received higher personal payments than other guideline authors. Of 861 references in the guidelines, 69 (8%) references were self-cited by the guideline authors, and 76% of the recommendations were on the basis of low or very low quality of evidence. There were no fully rigorous and transparent conflicts of interest policies for nephrology guideline authors in the United States, Europe, and Japan.

Conclusions
Most of the Japanese CKD guideline recommendations were on the basis of low quality of evidence by the guideline authors tied with pharmaceutical companies, suggesting the need for better financial conflicts of interest management.
Konsep Mahabbah dalam Al-Qur’an
Authors: Zuherni Zuherni , Ilyas Raudhatul Jannah 
Published: May 2022

Human love is nature from God. Mahabbah or love for fellow human beings in the form of love for parents, children, husband and wife and relatives is something that is natural, because of closeness and other emotional relationships. Moreover, love for them is based on love for Allah swt. However, all of this is forbidden if love for those closest to him turns him away from Allah, making him close his eyes in distinguishing between right and wrong. On the basis of this thought, it is necessary to study how to place the love of fellow human beings in accordance with the instructions of the Qur'an. The results of this paper indicate that love for fellow human beings both for parents, children, husband and wife and relatives in Islam is highly recommended, but if this love makes someone associate partners with Allah, commit immorality and disobey the Messenger of Allah, then love for fellow humans is not justified. Rasa cinta yang dimiliki manusia merupakan suatu fitrah dari Allah. Maḥabbah atau cinta sesama manusia berupa cinta kepada orang tua, anak, suami istri dan sanak saudara sesuatu yang alami, karena kedekatan dan hubungan emosional lainnya. Apalagi cinta kepada mereka didasari pada cinta kepada Allah swt. Akan tetapi, semua itu dilarang jika dengan cinta kepada orang-orang terdekat membuatnya berpaling dari Allah, membuat mata dia tertutup dalam membedakan antara yang benar dan yang salah. Atas dasar pemikiran inilah perlu dikaji bagaimana menempatkan cinta sesama manusia yang sesuai dengan petunjuk al-Qur’an. Hasil penulisan ini menunjukkan bahwa cinta sesama manusia baik kepada orang tua, anak, suami istri maupun sanak saudara di dalam Islam sangat dianjurkan, namun apabila cinta ini membuat seseorang menyekutukan Alah, berbuat maksiat serta ingkar kepada Rasulullah, maka rasa cinta kepada sesama manusia tidak dibenarkan.
Enhancing and Updating Cohen, Dey, and Lys’s (2008) Methodological Framework to Re-Examine the Relation between Accrual-Based and Real Earnings Management after SOX
Authors: Pincus Morton , Wu Shijia 
Published: May 2022

ABSTRACT
We respond to and rebut a number of comments in Daniel Cohen and Thomas Lys’s commentary (Cohen and Lys 2022) on our paper (Pincus et al. 2022), which re-examines and extends the research in Cohen, Dey, and Lys (2008), “(hereinafter CDL)”. Specifically, we rebut most of the claims Cohen and Lys (2022) make regarding the lack of a conceptual framework and research design limitations and clarify Cohen and Lys's (2022) misinterpretation on the inconsistency between our findings and prior published work. Cohen and Lys (2022) argue for the need to adjust the methodology that Cohen et al. (2008) employed but fail to acknowledge the enhancements we made to Cohen et al. (2008) methodological framework, which enabled us to separate the substitution effects associated with the pre-SOX and post-SOX periods.
Effect of De-intensifying Diabetes Medications on Negative Events in Older Veteran Nursing Home Residents
Authors: Niznik Joshua D. , Zhao Xinhua , Slieanu Florentina , Mor Maria K. , Aspinall Sherrie L. , Gellad Walid F. , Ersek Mary , Hickson Ryan P. , Springer Sydney P. , Schleiden Loren J. , Hanlon Joseph T. , Thorpe Joshua M. , Thorpe Carolyn T. 
Published: May 2022

<p><strong>Objective: </strong>Guidelines advocate against tight glycemic control in older nursing home (NH) residents with advanced dementia (AD) and/or limited life expectancy (LLE). We evaluated the effect of de-intensifying diabetes medications on all-cause emergency department (ED) visits, hospitalizations, and death in NH residents with LLE/AD and tight glycemic control.</p>
<p><strong>Research Design and Methods: </strong>We conducted a national retrospective cohort study of 2,082 newly admitted non-hospice Veteran NH residents with LLE/AD potentially overtreated for diabetes (HbA1c ≤7.5% and ≥1 diabetes medication) in fiscal years 2009-2015. Diabetes treatment de-intensification (dose decrease or discontinuation of a non-insulin agent or stopping insulin sustained ≥7 days) was identified within 30 days following HbA1c measurement. To adjust for confounding, entropy weights were used to balance covariates between NH residents who de-intensified vs. continued medications. We used the Aalen-Johansen estimator to calculate the 60-day cumulative incidence and risk ratios (RR) for ED or hospital visits and deaths. </p>
<p><strong>Results: </strong>Diabetes medications were de-intensified for 27%. In the subsequent 60 days, 28.5% of all residents were transferred to the ED or acute hospital setting for any cause and 3.9% died. After entropy weighting, de-intensifying was not associated with 60-day all-cause ED visits or hospitalizations (RR = 0.99 [0.84, 1.18]) or 60-day mortality (RR = 1.52 [0.89-2.81]). </p>
<p><strong>Conclusion: </strong>Among NH residents with LLE/AD who may be inappropriately overtreated with tight glycemic control, de-intensification of diabetes medications was not associated with increased risk of 60-day all-cause ED visits, hospitalization, or death in NH residents with LLE/AD.</p>
<p><br></p>
Targeting pancreatic islet NLRP3 improves islet graft revascularization
Authors: Wrublewsky Selina , Speer Thimoteus , Nalbach Lisa , Boewe Anne S. , Pack Mandy , Alansary Dalia , Roma Leticia P. , Hoffmann Markus D. A. , Schmitt Beate M. , Weinzierl Andrea , Menger Michael D. , Laschke Matthias W. , Ampofo Emmanuel 
Published: May 2022

<p> </p>
<p>Hypoxia-induced islet cell death, caused by an insufficient revascularization of the grafts, is a major obstacle for successful pancreatic islet transplantation. Recently, it has been reported that the nucleotide-binding oligomerization domain (NOD)-like receptor protein (NLRP)3 inflammasome is expressed in pancreatic islets and its loss protects against hypoxia-induced cell death. Therefore, we hypothesized that the inhibition of NLRP3 in islets improves the survival and endocrine function of the grafts. The transplantation of <em>Nlrp3</em>-/- islets or WT islets exposed to the NLRP3 inhibitor CY-09 into mouse dorsal skinfold chambers resulted in an improved revascularization when compared to controls. An increased insulin release after NLRP3 inhibition caused the enhanced angiogenic response. Moreover, the inhibition of NLRP3 in hypoxic β-cells triggered insulin gene expression by inducing the shuttling of MafA as well as pancreatic and duodenal homeobox (PDX)-1 into the nucleus. This was mediated by a reduced interaction of NLRP3 with the thioredoxin-interacting protein (TXNIP). Transplantation of <em>Nlrp3</em>-/- islets or WT islets exposed to CY-09 under the kidney capsule of diabetic mice markedly improved the restoration of normoglycemia. These findings indicate that the inhibition of NLRP3 in isolated islets represents a promising therapeutic strategy to improve engraftment and function of the islets.</p>
ANALISIS PAKET DATA DAN PERHITUNGAN KECEPATAN OBJECT DENGAN MENGGUNAKAN MODUL NODEMCU ESP8266 DAN SENSOR ULTRASONIC DENGAN LOCALHOST
Authors: Bhagaskoro M. Gilang 
Published: May 2022

Abstrak - Bandar Lampung merupakan ibu kota Provinsi Lampung yang merupakan pintu gerbang Pulau Sumatera. Hal ini tentu akan berpengaruh terhadap kondisi lalu lintas di Bandar Lampung yang akan dilalui oleh banyak kendaraan dari berbagai jenis transportasi darat, sehingga akan menyebabkan kemacetan yang cukup signifikan. Oleh karena itu, penulis mengembangkan sebuah sistem monitoring objek dengan sistem pengiriman data yang menggunakan modul NodeMCU ESP8266 berbasis sensor ultra sonic melalui jaringan 4G ke localhost, dengan parameter yang dianalisis adalah jumlah paket data dan kecepatan objek yang ada di perlintasan Jalan Sultan Agung Bandar Lampung. Skenario pada penelitian ini menggunakan 4 sensor ultrasonic dan sebuah controller yaitu NodeMCU ESP8266 yang digunakan untuk melakukan pengambilan data saat kendaraan melintas di jalan raya, maupun saat kendaraan dalam kondisi berhenti, lalu data dikirimkan menuju server dengan menggunakan XAMPP kemudian data yang telah diperoleh diekstrak. Berdasarkan hasil penelitian diperoleh hasil bahwa paket data yang hilang dengan nilai terkecil terjadi saat pengambilan data pada waktu siang hari tepatnya tanggal 21 Januari 2021 dengan nilai 28 paket atau persentase 14,66% dari jumlah paket keseluruhan pada sensor 2. Sementara, jumlah paket yang diterima paling banyak terjadi saat waktu siang hari tepatnya tanggal 21 Januari 2021 dengan jumlah 163 paket atau persentase sebesar 85,34% pada sensor 2.
Kata Kunci : Localhost, Microcontroller, NodeMCU ESP 8266, Sensing, Sensor Ultrasonic
Sistem Monitoring Instrument Air System Berbasis Internet Of Things di PT.Parna Raya
Authors: Nurfaizi Akhmad 
Published: May 2022

There is an operation process for facility & equipment support in the distribution process of the oil and gas company such as the Pressure Regulating System and Process Shutdown Valve. The supporting equipment uses pneumatic actuators as a driving force whose power source comes from air, as from the fact the compressor as an air producer is still manually used and the air pressure in the air receiver is not monitored from the control room. The purpose of this research is to develop the previous system by creating an integrated system that can be monitored remotely in real time by adding several electronic devices and utilizing Internet of Things technology. Based on the research that have been carried out, the pressure sensor has good accuracy with an average error of 0.06%, so it can be a reference in monitoring the pressure on the air receiver and a notification alarm will appear if the pressure on the air receiver reads 5.5 Bar as evidenced by testing 10 times the average pressure read on the blynk app of 5,460 Bar. Hardware and software function properly according to the design, air pressure at the air receiver and status of the compressor in stand-by or running state can be monitored through the blynk app, so that the gas distribution process does not stop again