bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis Cladophora rupestris absorption and response to Pb stress
Authors: Liu Lei , Zhang Lu-sheng , Yang Liu , Chen Qiu-yu , Zhang Qian , Cao Deju , Liu Zhao-wen 
Published: June 2022

Abstract
This study aimed to analyze the transcriptome of C. rupestris under Pb stress by using high-throughput sequencing technology, observe the changes of gene expression and metabolic pathway after 3 and 5 days under 1.0 and 5.0 mg/L Pb2+ treatment, and analyze the differentially expressed genes (DEGs) and related functional genes. Metabolic pathways were revealed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results show that DEGs increased significantly with the increase of Pb2+ concentration and stress time. A total of 32 genes were closely related to Pb stress response. GO analysis identified two major transporter proteins, namely, ATP-binding transport protein-related (ABC transporters) and zinc finger CCHC domain containing protein (Zfp) in C. rupestris. Pthr19248, pthr19211, Zfp pthr23002, Zfp p48znf pthr12681, Zfp 294 pthr12389, and Zfp pthr23067 played important roles against Pb infection and its absorption in C. rupestris. KEGG pathway analysis indicate that ABCA1, ATM, and ABCD3 were closely related to Pb absorption. Pb stress was mainly involved in metallothionein, plant hormone signal transduction, ABC transporters, and glutathione metabolism.
Towards holographic streaming systems: From real-time acquisition of the real world to holographic display
Authors: Yu Hyeonseung , Kim Youngrok , Yang Daeho , Seo Wontaek , Kim Yunhee , Hong Jong-Young , Song Hoon , Sung Geeyoung , Sung Younghun , Min Sung-Wook , Lee Hong-Seok 
Published: June 2022

Abstract
While recent progress in holographic displays has shown that they are capable of displaying photorealistic 3D holograms in real time, the acquisition of high-quality real-world holograms has remained a missing piece regarding the realization of holographic streaming systems. Incoherent holographic cameras, which record holograms under daylight conditions, are suitable candidates for real-world acquisition, as they avoid the safety issue involved in the use of laser lights; however, they are hindered by severe noise due to the optical imperfections of such systems. In this work, we develop a deep learning-based incoherent holographic camera system that can deliver visually enhanced holograms in real time. A neural network filters the noise in the captured holograms, maintaining a complex-valued hologram format throughout the whole process. Enabled by the computational efficiency of the proposed filtering strategy, we demonstrate the first holographic streaming system integrating a holographic camera and a holographic display, with the aim of developing the ultimate holographic ecosystem of the future.
Gender differences in willingness for childbirth, fertility knowledge, and value of motherhood or fatherhood and their associations among college students in South Korea
Authors: KIM Hae Won , KIM Seo Yun 
Published: June 2022

Abstract
Background
South Korea is a country with a very low fertility rate and there is a tendency for young adults to postpone marriage and childbirth, which affects adverse pregnancy outcomes. It is important for young adults to predict and prepare in advance for future fertility-related issues, especially by identifying their willingness and thoughts about childbirth for both women and men. This study aimed to investigate gender differences in willingness for childbirth, fertility knowledge, and the value of motherhood or fatherhood among college students in South Korea and to explore the factors influencing willingness for childbirth.
Methods
This was a cross sectional study among 286 unmarried college students who were recruited through a campus email and online communities for college student conducted from June 20, 2021 to July 19, 2021. The data were analyzed using the chi-square test and t-test to identify gender differences in general characteristics, willingness for childbirth, fertility knowledge, and value of motherhood of fatherhood. The factors influencing willingness for childbirth were examined using multiple logistic regression.
Results
Female students showed lower willingness for future childbirth than male students (χ2 = 26.85, p < .001). Women valued motherhood significantly less than men valued fatherhood (t = 6.34, p < .001). Women had a lower fertility knowledge score than men (t = 2.53 p = .012). The value of motherhood or fatherhood was an important influencing factor in both male and female college students (AOR = 8.57, 95% CI = 3.79–19.41 and AOR = 10.42, 95% CI = 3.65–29.80, respectively), whereas the monthly allowance was also an important influencing factor in female students (AOR = 1.02, 95% CI = 1.01–1.03).
Conclusions
The findings indicate the future direction to develop effective interventions considering gender differences which will promote healthy pregnancy and childbirth in the future for informed reproductive decision-making for college students.
Physician emigration from Nigeria and the associated factors: The implication to safeguarding the Nigeria Health System
Authors: Onah Cosmas Kenan , Azuogu Benedict Ndubueze , Ochie Casmir Ndubuisi , Akpa Christian Obasi , Okeke Kingsley Chijioke , Okpunwa Anthony Okoafor , Bello Hassan Muhammad , Ugwu George Onyemaechi 
Published: June 2022

Abstract
Background: Adequate Human Resources for Health is indispensable to achieving Universal Health Coverage and physicians play a leading role. Nigeria, with low physician-population ratio, is experiencing massive exodus of physicians. This study investigated emigration intention of physicians, the factors influencing it and discussed the implications to guide policy formulation and reforms to curtail the trend and safeguard the country’s health system.Methods: Through cross-sectional survey, 913 physicians from 37 States were interviewed with semi-structured questionnaire using online Google form with link shared via WhatsApp and Telegram forums of Nigeria Medical Association and her affiliate bodies. Data was analyzed with IBM-SPSS version-25 and charts were created with Microsoft Excel Spread Sheet version 2019. Chi-square and binary logistic regression tests were done with p-value set at 0.05 for statistical significance. Results: The mean age of respondents is 37.6±7.9 years; majority of them are males (63.2%), married (75.5%) with postgraduate qualifications (54.1%) and working in public health facilities (85.4%) located in urban areas (64.2%). Whereas only 13% and 19.3% of the physicians are respectively satisfied with their work and willing to continue practice in Nigeria, 43.9% want to emigrate and 36.8% are undecided about future location of their practice. The commonest reasons for emigration are poor remuneration (91.3%), rising insecurity (79.8%) and inadequate diagnostic facilities (61.8%). Physicians working in public health facilities are 2.5 times less satisfied than their counterparts in non-public sector and those in their thirties, forties and fifties are 3.5, 5.5 and 13.8 times respectively more willing to retain practice in Nigeria than those younger.Conclusion: Majority of Nigerian physicians want to emigrate for professional practice and top among the push factors are poor remuneration, rising insecurity and inadequate diagnostic facilities. The observed trend portends danger to the country’s health system due to the foreseeable negative consequences of physician deficit to the health system. We recommend upward review of physician remuneration, a root cause analysis of insecurity to determine workable preventive measures and increased funding of the health sector to improve the diagnostic infrastructure in order to retain physicians and save the health system from imminent collapse.
Cross-domain heterogeneous signcryption with keyword search for wireless body area network
Authors: Luo Ming , Huang Dashi , Qiu Minrong 
Published: June 2022

Abstract
In wireless body area network (WBAN), the WBAN sensors usually transmit encrypted data for the sake of security. But because of this, finding and getting the required data quickly becomes a challenge. Thus, many scholars have proposed searchable encryption (SE) schemes. Regrettably, many proposed SE schemes are not resistant to inside keyword guessing attack (IKGA), and most schemes are not suited for cross-domain communication in WBAN because they use single cryptosystem and the same cryptographic system parameters. To address the aforementioned issues, we put forward a new SE scheme for WBAN based on the public key cryptosystem in this paper. Our scheme allows WBAN sensors in certificateless public key cryptography (CLC) and receivers in public key infrastructure (PKI) environment use different cryptographic system parameters to realize cross-domain heterogeneous communication. At the same time, our scheme can effectively resist IKGA. Compared to the five existing schemes, the total computation cost of our scheme reduced by at least 59.99%.
Development of Human Pituitary Adenoma Organoids to Facilitate Effective Targeted Treatments of Cushing's Disease.
Authors: Zavros Yana , Chakrabarti Jayati , Pandey Ritu , Churko Jared , Eschbacher Jennifer , Mallick Saptarshi , Chen Yuliang , Hermes Beth , Mallick Palash , Stansfield Ben , Pond Kelvin , Thorne Curtis , Yuen Kevin , Little Andrew 
Published: June 2022

Abstract
Cushing's disease (CD) is a serious endocrine disorder caused by dysregulated adrenocorticotropic hormone (ACTH)-secreting pituitary tumor that stimulates the adrenal glands to overproduce cortisol. Chronic exposure to excess cortisol has detrimental effects on health, including increased stroke rates, diabetes, obesity, cognitive impairment, anxiety, depression, and death. The first-line treatment for CD is pituitary surgery, which is followed by disease remission. Current surgical remission rates reported range from 47–85% depending on several remission criteria. The lack of specificity, poor tolerability, and low efficacy of the subsequent second-line medical therapies makes CD a medical therapeutic challenge. One major limitation that hinders the development of specific medical therapies is the lack of human relevant model systems that recapitulate the cellular composition of pituitary adenomas. Human pituitary adenoma tissue was harvested during transsphenoidal surgery from CD patients to generate organoids (hPITOs). hPITOs generated from corticotroph, lactotroph, gonadotroph and somatotroph adenomas exhibited morphological diversity among the organoid lines between individual patients and amongst subtypes. The similarity in cell lineages between the organoid line and the patient’s tumor was validated by comparing the neuropathology report to the expression pattern of pituitary adenoma specific markers using spectral flow cytometry and exome sequencing. A high-throughput drug screen demonstrated patient-specific drug responses of hPITOs amongst each adenoma subtype. Generation of induced pluripotent stem cells (iPSCs) from patients carrying germline mutations relevant to CD exhibited dysregulated cell lineage commitment. The human pituitary adenoma organoids represent a novel approach in how we model complex pathologies in CD patients that may enable more effective personalized medicine for these patients.
Association between preterm births and socioeconomic development: analysis of national data
Authors: Montemor Marina Sanches , Demarque Gabriella Ferreira , Rodrigues Agatha Sacramento , Francisco Rossana Pulcinelli Vieira , Carvalho Mario Henrique Burlacchini 
Published: June 2022

Abstract
Background: The increasing prevalence of preterm birth, which is a global phenomenon, is attributable to the increased medical indications, artificial gestations, and some socioeconomic factors. This study was conducted to identify whether development and equality indices are associated with the incidence of preterm birth, specifically, spontaneous and elective preterm births.Methods: This retrospective observational study comprised an analysis of data on live births from 2019 in Brazil and on socioeconomic indices that were derived from census information in 2017. Data were summarised using absolute and relative frequencies. Spearman’s correlation was used to determine the correlation between socioeconomic factors and the preterm birth rate. Multiple beta regression analysis was performed to determine the best model of socioeconomic covariates and preterm birth rate. The significance level was set at 5%.Results: In 2019 in Brazil, the preterm birth rate was 11.03%, of which 58% and 42% were spontaneous and elective deliveries, respectively. For all preterm births, Spearman’s correlation varied from ρ = 0.4 for the Gini Index and ρ = −0.24 for illiteracy. The best fit modelled the spontaneous preterm birth fraction as a negative function of the Human Development Index (HDI). The best-fit model considered the expected elective preterm birth fraction as a positive function of the HDI and as a negative function of the Gini Index, which was used as a precision parameter.Conclusions: We observed a reduction in the fraction of spontaneous preterm births; however, the distribution was not uniform in the territory: higher rates of spontaneous preterm birth were noticed in the north, northeast, and mid-west regions. Thus, areas with lower education levels and inequal income distribution have a higher proportion of spontaneous preterm birth. The fraction of elective preterm birth was positively associated with more advantaged indices of socioeconomic status.
Dual isotopes tracing carbon and nitrogen dynamics during leguminous and non-leguminous litter decomposition under controlled precipitation
Authors: Du Ning ning , Qiu Liping , Zhang Yan jiang , Zhang Xing chang , Wei Xiao rong 
Published: June 2022

Abstract
Background and aims Plant litter decomposition figures importantly in the cycling of C and N pools in terrestrial ecosystems. We investigated how C and N fluxes changed during the decomposition of leaf litters and how those processes respond to different precipitation regimes.Methods We using dual isotope tracing and in situ decomposition methods, and investigated differences of leaf and soil C, N, δ13C and δ15N in northwest China.Results δ15N and δ13C were 3604‰ and 56‰ for Robinia pseudoacacia (Leguminosae) and 8115‰ and 452‰ for Populus ningshanica (Salicaceae) leaf litters. Through decomposition, δ13C decreased in all litters, and the δ15N of leguminous increased while non-leguminous litter decreased. In surface soil, the fractions of litter-derived C (20%) and N (14%) from leguminous were higher than non-leguminous litters after 16 months. C and N concentrations of both litters and soil were always coupled during decomposition, and the responses of N to C changes in soil were reduced by litter cover. Increased precipitation enhanced the litters’ C and N coupling. The 600-mm precipitation treatment most benefited litter C transformation to SOC, and drought conditions promoted the transformation of legume litter N to soil TN, but inhibited non-legume litter N. Conclusions C and N changes are always coupled in soil and litters. After 16 months, the proportion of C and N in soil from legumes was higher than non-legumes. Reduced precipitation can promote legumes N into soil. Our results provide a scientific basis for accurately predicting the C and N cycles in terrestrial ecosystems.
Practical self-management system for women with postnatal hypertension
Authors: Chang Chung-Wei , Tsai Yi-Jing , Hsu Yu-Yun , Hou Ting-Wei 
Published: June 2022

Abstract
High blood pressure would present in postpartum women diagnosed with hypertension during their perinatal period. Few studies are available regarding the self-management of postnatal hypertension via the eHealth system. This study aimed to develop a self-management eHealth system for women with postnatal hypertension during the postpartum period. We applied this system on multi-platform, not only on web interface but also on the platform of varied smartphones. The proposed system possessed three novel features: (1) Population was limited to postnatal women with hypertension; (2) self-care record, which allowed the postnatal women to keep tracking their blood pressure, pulse, weight, medication record, exercise record, and risk factor assessment; and (3) through this system, nurse-midwife could keep track of postnatal women's health status maintaining the complete record and could communicate directly with the users if their health monitor values reach beyond normal range. A survey was conducted among the postnatal women to evaluate the usability and acceptance of the eHealth system. The results showed that the overall satisfaction rate of the system reached 92.9% suggesting that the system was helpful to the users.
Mothers’ knowledge and self-reported performance regarding management of traumatic dental injuries: A cross-sectional study
Authors: Momeni Zahra , Afzalsoltani Sanaz , Moslemzadehasl Mohmoudreza 
Published: June 2022

Abstract
Background
Dental trauma is common among children. It has negative effects on a child’s quality of life. Parental knowledge, especially among mothers is an important factor for the long-term success of this emergency. This study aimed to investigate mothers' knowledge and self-reported performance, especially regarding the management of traumatic dental injuries.
Methods
In this cross-sectional study, 277 mothers of children (8–12 years) attending to pediatrics department of Alborz dental school were studied by convenience sampling. The independent variables were the child's gender, parents’ education and employment status, economic status, and inhabitation status. The dependent variable was the mothers’ knowledge and self-reported performance about the management of traumatic dental injuries. The data were collected using a valid and reliable questionnaire including four case scenarios of traumatic dental injury (case design) and analyzed by Linear multiple regression.
Results
The mean score of mothers’ knowledge about the management of traumatic dental injuries was 3.34 ± 1.58. The least obtained score in the section of knowledge was 0, and most of them were 7 (from a total score of 11). The mean score of mothers’ performance was 3.38 ± 1.2 (from a total score of 7). Results demonstrated that mothers' employment status and education influence their knowledge, while there is no relationship between mothers’ self-reported performance and the contextual variables (P < 0.05).
Conclusions
This finding demonstrated mothers have insufficient knowledge and performance about dental trauma management. The authors recommend giving effective academic intervention on knowledge and performance of dental trauma management at the community level, especially for parents.
Mass oscillations and matter wave’s phase and amplitude modulations of relativistic quantum particles induced by Heisenberg’s uncertainty principle
Authors: Tang Jau , Tang Qiang , Hu Zhi-Bin 
Published: June 2022

Abstract
We present a flip-flop dual model to treat quantum dynamics of relativistic particles with a rest mass and investigate the matter waves’ phase and amplitude modulations due to Heisenberg’s uncertainty principle. Their matter waves are shown to behave like a traveling wave packet accompanied by a guiding pilot wave, and the phase modulations result in mass oscillations. These effects are shown to be more prominent for light-weighted elementary particles, such as neutrinos and electrons. This mechanism is solely due to the uncertainty principle and has nothing to do with the flavor-mixing of neutrinos. Simulations using neutrinos and electrons are presented, which indicate an oscillation period on the order of ps. In this study, we primarily focus on the predicted mass oscillations induced by the uncertainty principle. A slit-type interference experiment using neutrinos and electrons from reactors is proposed to test the predicted behaviors.
Integrated transcriptomics contrasts fatty acid metabolism with hypoxia response in β-cell subpopulations associated with glycemic control
Authors: Miranda Mario A , Macias-Velasco Juan F , Schmidt Heather K , Lawson Heather A 
Published: June 2022

Abstract
Understanding how heterogeneous β-cell function impacts diabetes is imperative for therapy development. Standard single-cell RNA sequencing analysis illuminates some factors driving heterogeneity, but new strategies are required to enhance information capture. We integrate pancreatic islet single-cell and bulk RNA sequencing data to identify β-cell subpopulations based on gene expression and characterize genetic networks associated with β-cell function in obese SM/J mice. Previous studies have shown that high fat-fed SM/J mice resolve glycemic dysfunction between 20 and 30wks of age. We identify β-cell subpopulations associated with basal insulin secretion, hypoxia response, cell polarity, and stress response. Relative proportions of these cells are influenced by age, sex, and diet. Network analysis associates fatty acid metabolism and basal insulin secretion with hyperglycemic-obesity, while expression of Pdyn and hypoxia response is associated with normoglycemic-obesity. By integrating single-cell and whole islet transcriptomes, our study explores β-cell heterogeneity and identifies novel subpopulations and genetic pathways associated with β-cell function in obesity.
Enhanced poly-γ-glutamic acid production by a newly isolated Bacillus tequilensis BL01
Authors: Wang Dexin , Zhou Dasen , Gao Jiaqi , Bai Wenqin 
Published: June 2022

Abstract
Poly γ-glutamic acid (γ-PGA) is a promising biopolymer for various applications. In this study, we isolated a novel γ-PGA-producing strain, Bacillus tequilensis BL01. The one-factor-at-a-time method was used to investigate the influence of carbon and nitrogen sources and temperature on γ-PGA production. The optimal carbon and nitrogen sources were sucrose and (NH4)2SO4, respectively. The optimal temperature for γ-PGA production was determined to be 37°C. Response surface methodology was used to determine the optimum medium components: 68.9 g/L sucrose, 47.7 g/L monosodium glutamate, and 2.5 g/L K2HPO4. γ-PGA titer increased significantly from 8.6 g/L to 21.1 g/L when strain BL01 was cultivated in the optimized medium. Furthermore, γ-PGA titer reached 54.7 g/L with a productivity of 1.37 g/L/h and a yield of 2.47 g of γ-PGA/g of L-glutamic acid with the optimized medium in fed-batch fermentation. It should be noted that the γ-PGA yield in this study was the highest of all reported studies, indicating a great potential for the industrial production of γ-PGA.