bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct Transcriptomic and Proteomic Profile Specifies Patients Who Have Heart Failure With Potential of Myocardial Recovery on Mechanical Unloading and Circulatory Support
Authors: Drakos Stavros G. , Badolia Rachit , Makaju Aman , Kyriakopoulos Christos P. , Wever-Pinzon Omar , Tracy Christopher M. , Bakhtina Anna , Bia Ryan , Parnell Timothy , Taleb Iosif , Ramadurai Dinesh K.A. , Navankasattusas Sutip , Dranow Elizabeth , Hanff Thomas C. , Tseliou Eleni , Shankar Thirupura S. , Visker Joseph , Hamouche Rana , Stauder Elizabeth L. , Caine William T. , Alharethi Rami , Selzman Craig H. , Franklin Sarah 
Published: November 2022

Background:
Extensive evidence from single-center studies indicates that a subset of patients with chronic advanced heart failure (HF) undergoing left ventricular assist device (LVAD) support show significantly improved heart function and reverse structural remodeling (ie, termed “responders”). Furthermore, we recently published a multicenter prospective study, RESTAGE-HF (Remission from Stage D Heart Failure), demonstrating that LVAD support combined with standard HF medications induced remarkable cardiac structural and functional improvement, leading to high rates of LVAD weaning and excellent long-term outcomes. This intriguing phenomenon provides great translational and clinical promise, although the underlying molecular mechanisms driving this recovery are largely unknown.

Methods:
To identify changes in signaling pathways operative in the normal and failing human heart and to molecularly characterize patients who respond favorably to LVAD unloading, we performed global RNA sequencing and phosphopeptide profiling of left ventricular tissue from 93 patients with HF undergoing LVAD implantation (25 responders and 68 nonresponders) and 12 nonfailing donor hearts. Patients were prospectively monitored through echocardiography to characterize their myocardial structure and function and identify responders and nonresponders.

Results:
These analyses identified 1341 transcripts and 288 phosphopeptides that are differentially regulated in cardiac tissue from nonfailing control samples and patients with HF. In addition, these unbiased molecular profiles identified a unique signature of 29 transcripts and 93 phosphopeptides in patients with HF that distinguished responders after LVAD unloading. Further analyses of these macromolecules highlighted differential regulation in 2 key pathways: cell cycle regulation and extracellular matrix/focal adhesions.

Conclusions:
This is the first study to characterize changes in the nonfailing and failing human heart by integrating multiple -omics platforms to identify molecular indices defining patients capable of myocardial recovery. These findings may guide patient selection for advanced HF therapies and identify new HF therapeutic targets.
MicroRNA-195a-5p Regulates Blood Pressure by Inhibiting NKCC2A
Authors: Hao Shoujin , Zhao Hong , Hao David H. , Ferreri Nicholas R. 
Published: November 2022

Background:
Previous studies showed that miR-195a-5p was among the most abundant microRNAs (miRNAs) expressed in the kidney.

Methods:

Lentivirus silencing of tumor necrosis factor-α (TNF) was performed in vivo and in vitro. Luciferase reporter assays confirmed that bumetanide-sensitive Na
+
-K
+
-2Cl
−
cotransporter isoform A (NKCC2A) mRNA is targeted and repressed by miR-195a-5p. Radiotelemetry was used to measure mean arterial pressure.

Results:

TNF upregulates mmu-miR-195a-5p, and -203 and downregulates mmu-miR-30c and -100 in the medullary thick ascending limb of male mice. miR-195a-5p was >3-fold higher in the renal outer medulla of mice given an intrarenal injection of murine recombinant TNF, whereas silencing TNF inhibited miR-195a-5p expression by ≈51%. Transient transfection of a miR-195a-5p mimic into medullary thick ascending limb cells suppressed NKCC2A mRNA by ≈83%, whereas transfection with Anti-miR-195a-5p increased NKCC2A mRNA. Silencing TNF in medullary thick ascending limb cells prevented increases in miR-195 induced by 400 mosmol/kg H
2
O medium, an effect reversed by transfection with a miR-195a-5p mimic. Expression of phosphorylated NKCC2 increased 1.5-fold in medullary thick ascending limb cells transfected with Anti-miR-195a-5p and a miR-195a-5p mimic prevented the increase, which was induced by silencing TNF in cells exposed to 400 mosmol/kg H
2
O medium after osmolality was increased by adding NaCl. Intrarenal injection of TNF suppressed NKCC2A mRNA, whereas injection of miR-195a-5p prevented the increase of NKCC2A mRNA abundance and phosphorylated NKCC2 expression when TNF was silenced. Intrarenal injection with miR-195a-5p markedly attenuated MAP after renal silencing of TNF in mice given 1% NaCl.

Conclusions:
The study identifies miR-195a-5p as a salt-sensitive and TNF-inducible miRNA that attenuates NaCl-mediated increases in blood pressure by inhibiting NKCC2A.
The Soaring Eagle Academy: the Inception of a Comprehensive Post-Secondary Transition Program for Individuals with Intellectual Disability in Southwest Florida
Authors: Halpern Clarisse , Carothers Douglas , Aydin Hasan 
Published: November 2022

Abstract
Higher education institutions committed to values of diversity, inclusion, and equity offer programs and practices that support individuals of diverse populations, including those with disabilities. Passage of the Higher Education Opportunity Act in the United States expanded access to individuals with intellectual disabilities (id) wishing to pursue higher education in Comprehensive Transition Programs (ctp s). Despite previous publications addressing the benefits of establishing such programs, little has been found regarding steps to develop these programs. Thus, this paper describes the case of the inception of the Soaring Eagle Academy (sea), a ctp for students with id in a Southwest Florida university, United States. Multiple data sources were collected and analyzed with content analysis. The findings emphasized the importance of having a collaborative team empowered and supported by the university administration to create a life-changing program that would impact students with id, their families, the campus culture, and the community. Recommendations for future research are included.
Music in the Operating Room: Comparing the Opinions of Surgeons, Anesthesiologists, and Nurses
Authors: Lamberton Tessa , de Virgilio Christian , Terrell James , Moazzez Ashkan , Combs Kristen , Nafday Revati , Salazar Daniel , Yazdany Tajnoos , Calhoun Colonya , Yetasook Amy 
Published: November 2022

Background Music is part of operating room (OR) culture; however, some personnel may perceive music as a distraction. Methods A single institution survey of surgeons (SURG), anesthesia (ANES), and nursing (NURS) regarding attitudes on music in the OR. Results There were 222 responses (67% response rate) agreeing that music in the OR should be allowed (91%), is calming (75%), and helps with focus (63%). Most did not feel music was distracting (63%) or unsafe (80%). SURG were more likely to state that surgeons should decide (46.7%) if music should be played, whereas ANES and NURS (81%) were more likely to feel decisions should be made collaboratively (P < .001). Conclusion Most OR personnel feel positively towards music. Surgeons were more likely to believe the decision to play music should be the surgeon’s choice. The majority of OR staff agreed with collaborative decision-making, aligning with creating a safe OR culture.
Mechanical Ventilation Does Not Predict Pneumothorax Observation Failure in the Severely Injured
Authors: Pokrzywa Courtney J. , Figueroa Juan , Gomez Jose , Karam Basil , Murphy Patrick , Iverson Katie , Morris Rachel , Carver Tom , Milia David , de Moya Marc 
Published: November 2022

Background Observative management of small traumatic pneumothoraces (PTX) has been shown to decrease chest tube utilization in non-mechanically ventilated patients without compromising outcomes. This approach could be used in mechanically ventilated (MV) patients, though many feel these patients are at increased risk of observation failure. Methods A single center retrospective study of all adults undergoing observation of a computed tomography (CT) diagnosed PTX from 2015-2019. Patients with chest tube placement within 4-hours of arrival, concurrent hemothorax, or death within 24-hours were excluded. Observation failure was defined as chest tube placement. Results Of 340 patients, 64 were on MV. The groups were of similar age, BMI, underlying pulmonary comorbidities, and PTX size (10.1 mm vs 8.8 mm, P = .20). The MV group was more severely injured (ISS [25+] [60.9% vs 11.2%, P < .001]). There was no difference in observation failure rates by MV status overall (6.3% vs 5.1%, P = .75) or by PTX size (<15 mm [5% vs 2.2%, P = .37], <20 mm [4.8% vs 3.1%, P = .45], <25 mm [4.8% vs 4.1%, P = .73], <30 mm [4.8% vs 4.1%, P = .73], <35 mm [4.8% vs 4.7%, P = 1.00]). MV was not an independent predictor of observation failure on multivariable analysis (OR .64, 95% CI .18-2.20), though PTX size was (OR 1.11, 95% CI 1.05-1.17). When comparing those who failed vs those who did not, the only difference was PTX size (9.34 mm vs 19.41 mm, P < .001). Conclusion MV is not an independent predictor of PTX observation failure. While PTX size appears to play a role, further studies are needed to outline safe parameters for observation in those undergoing MV.
A Novel Method for Making an Auricle Framework Like Lego Building Blocks
Authors: Sun Pengfei , Yuan Limei , Yang Yang , Zhu Lingdong 
Published: November 2022

Objectives The traditional Tanzer-Brent method, which uses the integral engraving of costal cartilage, provides insufficient three-dimensional sensation and is characterized by poor firmness of the auricle framework. This tends to cause the absorption deformation of the auricle framework after surgery. In order to reduce the absorption deformation as well as make the reconstructed auricle more three-dimensional and the ear shape more realistic, our ear reconstruction team invented a novel method to make an auricle framework with autologous costal cartilage. The auricle framework made by this novel method has been applied to auricle reconstruction and has achieved excellent surgical results. Patients and Methods Clinical data and postoperative data were collected for patients with unilateral microtia who received auricle reconstruction using the novel method of auricle framework construction in Plastic Surgery Hospital from January 2020 to June 2022. The therapeutic effect of this novel method was evaluated by the satisfaction rate of patients and their families and by the prevalence of postoperative complications. Results A total of 60 patients were enrolled in this study. The average age was (10.95 ± 2.97) years old. The mean follow-up time was 24.02 ± 1.52 months. The satisfaction rate of patients and their families was 96.7%. In terms of postoperative complications, two patients had postoperative infection, one patient had framework exposure, no patients had framework absorption and deformation, two patients had postoperative hematoma, and three patients had scar hyperplasia. Conclusion This novel method of making the auricle framework is more standardized, easier to master for plastic surgeons, and more conducive to the clinical application of plastic surgery. Through this study, we believe that this novel method offers significant therapeutic advantages and can be used as a unified standard for construction of an auricle framework using autologous costal cartilage.
Effects of Tele-Counseling on Reducing Anxiety Levels of COVID-19 Patients in Isolation Wards: An Observational Study
Authors: Ganesan Sivaraman , Balasubramanian Bhuvaneshwaran , Krishnamurthy Pugazhendhi , Govindan Ramanujam , Mani Nanthakumar 
Published: November 2022

Background: COVID-19 causes psychological distress and anxiety due to fear of infection and the possibility of worsening symptoms leading to mortality. Public health measures like contact tracing and lockdowns further increase the panic among infected patients and the public. We intended to assess the effect of telecounseling in reducing anxiety in patients admitted to isolation wards. Methods: In this multi-center observational study, we administered a Generalized Anxiety Disorder-7 (GAD-7) scale before and after telecounseling. The study group consisted of participants who gave consent for telecounseling, and those who did not give consent constituted the control group. The telecounseling was based on the Psychological First Aid model, due to its simplicity. Results: There was a statistically significant decrease in anxiety after telecounseling in the study group (P value ≤ 0.001, effect size = 0.484). There was a statistically significant decrease in anxiety in the study group compared to the control group (P value ≤ 0.001). Conclusion: Telecounseling contributed to the reduction in anxiety in COVID-19 patients in isolation wards. Several other factors like severity of the infection, comorbid medical illness, pre-existing mental health issues, individual’s level of coping with stress, and duration and place of admission may have affected the anxiety levels in the patients.
Depression, Anxiety, and Quality of Life in Patients Treated with Single Infusion Tocilizumab for COVID-19: A Follow-up, Controlled Study
Authors: Chakravarty Rahul , Jyani Gaurav , Paul Samita , Mohindra Ritin , Goyal Manoj , Suri Vikas , Bhalla Ashish , Singh Shubh Mohan 
Published: November 2022

Background: The inflammatory state that characterizes COVID-19 may contribute to depression, anxiety, other morbidities, and mortality. Interleukin-6 blocker tocilizumab has been used as a treatment modality in COVID-19 as an anti-inflammatory agent. Tocilizumab has also been studied for its potential use in preventing depression in other patient groups, with mixed results. This study was designed to study subsequent depression, anxiety, and quality of life (QOL) in patients treated with tocilizumab plus standard care versus standard care alone, for moderate to severe COVID-19 pneumonia. Methods: Patients admitted with moderate to severe COVID pneumonia, treated with tocilizumab plus standard treatment, and discharged from the hospital were identified. Age- and gender-matched controls who had received standard treatment alone for COVID-19 pneumonia during the same time frame were identified. After obtaining consent, these patients were followed up and assessed at 3 and 6 months on measures of depression (PHQ-9), anxiety (GAD-7), and QOL (EQ-5D-5L). Results: 39 patients in the tocilizumab group and 41 in the control group were followed up at 3 and 6 months. Patients in both groups were comparable in sociodemographic and clinical parameters. The prevalence of clinically significant depression in the tocilizumab group at 3 and 6 months was 33.33% and 5.12%, respectively, whereas in control group it was 31.7% and 4.87%, respectively. Analysis of EQ-5D health profiles revealed that the maximum problems were reported in the dimension of mobility: 43.7% at 3 months and 35% at 6 months. Conclusions: Depressive and anxiety symptoms and impairment in QOL were reported more frequently and with greater severity in patients in the tocilizumab group at three months but not at six months. Psychological morbidity and impairment in QOL were modest and improved from 3 to 6 months.
Rationally Inattentive Behaviour with A Priori Knowledge
Authors: Dvořák Jiří , Votava Libor , Komárková Lenka , Sabolovic Mojmír , Pospíšil Jan Závodný 
Published: November 2022

Purpose: To contribute to the discussion on the importance of the sense of fairness in the decision-making process according to the theory of rational inattention. Design: The study is based on an experiment where economically educated respondents state and justify the price at which they are willing to provide and accept help in an emergency. Findings: Most of the respondents are willing to provide and accept help. For the assistance provided, most would demand a significantly lower price than the maximum economic return defined by the known value of the commercial service. The respondents were repeatedly willing to pass on the pricing decision to the counterparty. Practical Implications: This behaviour in decision-making processes represents non-inclusion of a sufficient a priori knowledge as a determining factor in the selection of variants, which is a limitation in the cognitively limited modelling of economic subjects. Originality/Value: The results are based on the original experiment as an empirical basis and it is valuable because of the lack of experimental research in this domain. Limitations include the relatively small sample of respondents and, in terms of generalising the results, that they were students at a university of economics. JEL: D01, D11, D12, D14
A multicenter phase 2 single arm study of cabozantinib in patients with advanced or unresectable renal cell carcinoma pre-treated with one immune-checkpoint inhibitor: The BREAKPOINT trial (Meet-Uro trial 03)
Authors: Procopio Giuseppe , Claps Mélanie , Pircher Chiara , Porcu Luca , Sepe Pierangela , Guadalupi Valentina , De Giorgi Ugo , Bimbatti Davide , Nolè Franco , Carrozza Francesco , Buti Sebastiano , Iacovelli Roberto , Ciccarese Chiara , Masini Cristina , Baldessari Cinzia , Doni Laura , Cusmai Antonio , Gernone Angela , Scagliarini Sarah , Pignata Sandro , de Braud Filippo , Verzoni Elena 
Published: November 2022

Background: First-line therapies based on immune-checkpoint inhibitors (ICIs) significantly improved survival of metastatic renal cell carcinoma (mRCC) patients. Cabozantinib was shown to target kinases involved in immune-escape and to prolong survival in patients pre-treated with tyrosine-kinase-inhibitors (TKIs). The impact of ICIs combinations in first line on subsequent therapies is still unclear. Methods: This is an open label, multicenter, single arm, phase II study designed to assess activity, safety and efficacy of cabozantinib in mRCC patients progressed after an adjuvant or first line anti-Programmed Death (PD)-1/PD-Ligand (PD-L) 1-based therapy. Primary endpoint was progression free survival (PFS), secondary endpoints were overall survival (OS), objective response rate (ORR) and safety. Results: 31 patients were included in the analysis. After a median (m) follow-up of 11.9 months, mPFS was 8.3 months (90%CI 3.9-17.4) and mOS was 13.8 months (95%CI 7.7-29.0). ORR was 37.9% with an additional 13 patients achieving disease stability. Grade 3-4 adverse events occurred in 47% of patients, including more frequently creatine phosphokinase (CPK) serum level elevation, neutropenia, hyponatremia, diarrhea, hand-food syndrome, oral mucositis and hypertension. Conclusions: The BREAKPOINT trial met its primary endpoint showing that cabozantinib as second line therapy after ICIs was active in mRCC. Safety profile was manageable. Trial registration number: NCT03463681 - A Study of CaBozantinib in Patients With Advanced or Unresectable Renal cEll cArcinoma (BREAKPOINT) - https://clinicaltrials.gov/ct2/show/NCT03463681
Acute correction of distal femoral deformities by retrograde femoral nail using preoperative planning
Authors: Kariksiz Mesut , Karakoyun Ozgur 
Published: November 2022

Purpose This study presents the technique and results of acute correction of distal femoral deformities using retrograde femoral nailing based on preoperative planning without using a fixator. Methods Twenty-eight patients (34 limbs: 22 left-sided, 12 right-sided, mean age = 36 years) undergoing distal femoral deformity correction with retrograde femoral nailing between 2013 and 2020 were examined retrospectively. The osteotomy line, block screw location and number were identified by detailed preoperative planning. Osteotomy was performed using the percutaneous multiple drill method, and the retrograde femoral nail was placed. Results The average follow-up period was 62.7 months (range: 13–84 months). Postoperatively, the mean mechanical axis deviation was corrected to 6.8 mm (range: 0–8 mm) and the mean mechanical lateral distal femoral angle to 87.42° (range: 84–90°). The Association for the Study an Application of the Method of Ilizarov (ASAMI) score was excellent for all patients. None of the patients had fracture, infection, implant failure, or non-union. Conclusion Distal femoral deformities can be corrected acutely, without applying external fixators, with good preoperative planning and using the retrograde femoral nail. This technique is safe and effective, with a low complication rate.
Serum NfL but not GFAP predicts cognitive decline in active progressive multiple sclerosis patients
Authors: Barro Christian , Healy Brian C , Saxena Shrishti , Glanz Bonnie I , Paul Anu , Polgar-Turcsanyi Mariann , Guttmann Charles RG , Bakshi Rohit , Weiner Howard L , Chitnis Tanuja 
Published: November 2022

Background: Cognitive decline is inadequately captured by the standard neurological examination. Serum neurofilament light chain (sNfL) and glial fibrillary acidic protein (sGFAP) are biomarkers of neuronal damage and astrocytic reactivity that may offer an accessible measure of the multiple sclerosis (MS) pathology linked to cognitive decline. Objective: To investigate the association of sNfL and sGFAP with cognitive decline in MS patients at high risk for progressive pathology. Methods: We included 94 MS patients with sustained Expanded Disability Status Score (EDSS) ⩾ 3, available serum samples and cognitive assessment performed by symbol digit modalities test (SDMT) over a median of 3.1 years. The visit for sGFAP/sNfL quantification was at confirmed EDSS ⩾ 3. Linear regression analysis on log-transformed sGFAP/sNfL assessed the association with current and future SDMT. Analyses were adjusted for age, sex, EDSS, treatment group, and recent relapse. Results: sNfL was significantly associated with concurrent SDMT (adjusted change in mean SDMT = −4.5; 95% confidence interval (CI): −8.7, −0.2; p = 0.039) and predicted decline in SDMT (adjusted change in slope: −1.14; 95% CI: −1.83, −0.44; p = 0.001), particularly in active patients. sGFAP was not associated with concurrent or future SDMT. Conclusions: Higher levels of sNfL were associated with cognitive impairment and predicted cognitive decline in MS patients at high risk for having an underlying progressive pathology.