bluuebunny/crossref_metadata_2025_split huggingface dataset:
How environmental conditions impact seed germination and consequently the invasion strategy of glossy buckthorn (Frangula alnus (Mill)) in Eastern Canada
Authors: Custodio Tiana Mara , Houle Daniel , Girard François 
Published: December 2022

Abstract
Identifying the environmental factors that control germination is essential to understanding seed traits and their effect on fitness. Glossy buckthorn ( Frangula alnus ) is a shrub or small tree from Eurasia that has become invasive in North America, and which has negative impacts on plant communities and ecosystems. In this study, we analyzed the germination response of glossy buckthorn seeds to different temperatures, various stratification lengths and scarification conditions to measure the impact on breaking seed dormancy, as well as the effect of light in triggering germination. Survival analysis was performed utilizing a Kaplan-Meier estimator and Cox proportional hazard model. Glossy buckthorn seeds exhibited physiological dormancy and required cold stratification to germinate. Germination starts at the end of spring when temperatures reach 12°C and reach a peak later in the season with a germination rate of 70% at 20°C. At warmer temperatures, germination declines, and seeds are induced into dormancy again. Ungerminated seeds will form persistent seed banks that will ensure the survival of F. alnus over time and space as well as enhance its chances of a successful establishment and invasion. Due to climate change, increases in temperatures might impact seeds buried in the soil, shorten the length of stratification periods in some areas of North America and consequently affect the timing of germination resulting in a shift of its germination period.
Computational analysis of missense variant CYP4F2*3 (V433M) in association with human CYP4F2 dysfunction: A functional and structural impact
Authors: Dehkordi Mahvash Farajzadeh , Mafakher Ladan , Samiee-Rad Fatemeh , Rahmani Babak 
Published: December 2022

Abstract
Background
Cytochrome P450 4F2 (CYP4F2) enzyme is one of the CYP4 family responsible for the metabolism of fatty acids, therapeutic drugs, and signaling molecules, such as arachidonic acid, tocopherols, and vitamin K. Several reports have demonstrated that missense variant CYP4F2*3 (V433M) cause decreased activity of CYP4F2 and inter-individual variations in warfarin dose in different ethnic groups. However, the molecular pathogenicity mechanism of missense V433M on the CYP4F2 protein at the atomic level has not yet been completely elucidated.
Methods and results
In the current study, we evaluate the effect of V433M substitution on the CYP4F2 enzyme through 11 different bioinformatics tools. Further molecular dynamics (MD) simulation was applied to assess the impact of V433M mutation on CYP4F2 protein structure, stability, and dynamics. Also, the molecular docking method was performed to illustrate the effect of V433M on its interaction with vitamin K. Based on our results, the CYP4F2*3 variant is a damaging polymorphism with destabilizing nature. Simulation results presented that missense V433M affects the dynamics and stability of CYP4F2 by reducing its compactness and stability, which means its tendency to change the overall structural conformation and flexibility of CYP4F2. The docking result showed that the CYP4F2*3 variant decreased binding affinity between vitamin K1 and CYP4F2 enzyme, which caused less activity of CYP4F2*3 compared to native CYP4F2.
Conclusions
This investigation determined the molecular pathogenicity mechanism of the CYP4F2*3 variant on the human CYP4F2 protein and supplied new information for comprehending the structure-function relationship in CYP4F2 and other CYP4 enzymes. These findings will help to develop effective drugs and individual treatment options.
Environmentally Friendly Concrete Block Production: Valorization of Civil Construction and Chemical Industry Waste
Authors: Junkes Valderice H , Fuziki Maria Eduarda K , Tusset Angelo Marcelo , Rodrigues Paulo Henrique , Lenzi Giane Gonçalves 
Published: December 2022

Abstract
In the present work, a study was carried out on the dosage of wastes from civil construction (concrete and plaster) and chemical industry (tannery sludge) in mixtures for the production of concrete blocks. The characterization of the materials used was performed employing X-ray diffractometry (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). The influence of the different residues in the blocks properties was evaluated from the tests of compressive strength, traction in bending, water absorption and leaching test. The results indicated that the concrete blocks produced for paving with addition of residues did not obtain gains in the values of mechanical resistance to compression and traction in bending when compared with blocks made with standard raw material. However, the blocks produced with construction waste obtained satisfactory results for use in street paving after 7 days of concrete curing, reaching values between 36.54 MPa and 44.6 MPa for the mentioned properties. It was also observed that these values increase to 21.4% within 28 days of cure. The blocks produced with plaster showed values between 37.03MPa and 39.85MPa after 28 days of curing, allowing their use for paving streets. On the other hand, the blocks produced with residues from the chemical industry had lower strengths, reaching a maximum power of 29.36MPa after 28 days of curing. In addition, it was also noted that the blocks produced with recycled concrete showed an improvement in performance with a recycled quantity 50%.
Development and validation of a Japanese language questionnaire to screen for tension-type headaches and migraines
Authors: Tanobe Kaho , Machida Minori , Motoya Ryo , Takeoka Astushi , Danno Daisuke , Miyahara Junichi , Takeshima Takao , Kumano Hiroaki , Tayama Jun 
Published: December 2022

Abstract
Background Tension-type headaches and migraines are prevalent conditions; however, no reliable biological markers have been identified for screening. A validated screening tool for epidemiological studies would aid in carrying out large-scale epidemiological studies. Therefore, there is a need to develop a screening tool in the Japanese language based on the International Classification of Headache Criteria, which can be used in epidemiological studies. In this study, we aimed to develop a Japanese-language tool that can be used to screen patients for migraine, tension-type headaches, and mixed migraine/tension-type headaches. Consequently, our objective was to develop a questionnaire based on the 3rd edition of the International Classification of Headache Disorders (ICHD-3) using screening items for each type of headache and to examine its validity.
Methods The questionnaire consisted of 29 questions based on the ICHD-3 diagnostic criteria. It comprised 10 screening items for migraine, eight for tension-type headache, and five for mixed migraine/tension-type headache. The screening results of the questionnaire were compared with the diagnoses of three neurologists, which were used as the gold standard reference.
Results The study population comprised 69 patients (mean age ± standard deviation, 55.0 ± 18.7 years) aged 19–89 years who were visiting the Tominaga Hospital of the Kotobukai Social Medical Corporation. According to the neurologists’ diagnoses, twenty-two patients had migraine, 30 had tension-type headaches, and 17 had mixed migraine/tension-type headaches. The sensitivity and specificity were as follows: migraine, 72.7% and 86.7%; tension-type headache, 50.0% and 86.4%; and mixed migraine/tension-type headache, 70.6% and 67.3%, respectively.
Conclusions The screening tool developed in this study has sufficient validity except for tension-type headache. The questionnaire developed in this study is a rapid and sensitive tool for determining migraine, tension-type headache, and mixed migraine/tension-type headache in persons with headache symptoms.
XPF-ERCC1 blocker improves the therapeutic efficacy of 5-FU- and oxaliplatin-based chemoradiotherapy in rectal cancer
Authors: Huang Ming-Yii , Huang Yi-Jung , Cheng Tian-Lu , Jhang Wun-Ya , Ke Chien Chih , Chen Yi-Ting , Kuo Shih-Hsun , Lin I-Ling , Huang Yu-Hsiang , Chuang Chih-Hung 
Published: December 2022

Abstract
5-FU-based chemoradiotherapy (CRT) and oxaliplatin-based CRT are commonly used therapies for advanced rectal cancer (RC). However, patients with high expression of ERCC1 have worse prognosis than those with low expression. In this study, we investigated the effect of XPF-ERCC1 blockers on chemotherapy and 5-FU-based CRT and oxaliplatin (OXA)-based CRT in rectal cancer cell lines. We investigated half maximal inhibitory concentration (IC50) of 5-FU, OXA, XPF-ERCC1 blocker, and XPF-ERCC1 blocker and 5-FU or OXA combined, and analyzed the effect of XPF-ERCC1 blocker on 5-FU-based CRT and oxaliplatin-based CRT. Furthermore, the expression of XPF and γ-H2AX in rectal cells was analyzed. In animal models, we combined XPF-ERCC1 blocker with 5-FU and OXA to investigate the effects of RC, and finally combined XPF-ERCC1 blocker with 5-FU- and oxaliplatin-based CRT. In the IC50 analysis of each compound, the cytotoxicity of the XPF-ERCC1 blocker was lower than that of 5-FU and OXA. In addition, XPF-ERCC1 blocker combined with 5-FU or OXA enhanced the cytotoxicity of the chemotherapy drugs in rectal cells. Furthermore, the XPF-ERCC1 blocker also increased the cytotoxicity of 5-FU-based CRT and OXA -based CRT by inhibiting the XPD product DNA locus. In vivo, the XPF-ERCC1 blocker was confirmed to enhance 5-FU, OXA, 5-FU-based CRT, and OXA CRT. These findings show that XPF-ERCC1 blockers cannot only increase the toxicity of chemotherapy drugs, but also increase the efficacy of combined chemoradiotherapy. In the future, the XPF-ERCC1 blocker may be used to improve the efficacy of 5-FU- and oxaliplatin-based CRT.
The average gonadotrophin dosage per follicle is predictive of ovarian response and cumulative live birth chances after in vitro fertilization: a retrospective cohort study
Authors: Liu Shan , Ma Shuai , Li Yuan 
Published: December 2022

Abstract
Background: With the development of assisted reproduction technology (ART), many indicators have been proposed to evaluate ovarian response, and then predict pregnancy outcomes. In general, the predictive values remain limited.
Objective: To further explore the indicators to evaluate ovarian sensitivity to gonadotrophin (Gn) stimulation more accurately.
Methods: This retrospective cohort study included 330 women who underwent an entire ART cycle. We aimed to assess whether a new index, termed as average Gn dosage per follicle, could be used as a marker for ovarian response and pregnancy outcomes. It was calculated as the ratio of total Gn dose during ovarian stimulation and the number of pre-ovulatory follicles on the trigger day. Patients were divided into three subgroups according to the average Gn dosage per follicle: below the 33rd percentile (Group A), between 33rd and 67th percentiles (Group B), and above the 67th percentile (Group C). Then stimulation data, laboratory and clinical outcomes were compared among the groups.
Results: The results showed patients in Group A had the best ovarian response, the number of retrieved oocytes was significantly higher than in Group B and C. A multivariate regression analysis showed that average Gn dosage per follicle was an independent predictor of cumulative live birth rates (CLBRs) [adjusted odds ratio (OR): 0.96, 95% confidence interval (CI): 0.95-0.98, P < 0.01].
Conclusions: The present study showed that average Gn dosage per follicle appears to be a highly reliable index of ovarian response to exogenous Gn and can be useful to estimate CLBR.
Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway
Authors: Hao Junfeng , Chen Qiguang , Feng Yongmin , Jiang Qiyu , Sun Huiwei , Deng Botian , Huang Xin , Guan Jibin , Chen Qiuping , Liu Xincheng , Wang Yanjin , Cao Peng , Feng Fan , Li Xiaoyu 
Published: December 2022

AbstractFerroptosis, a nonapoptotic form of programmed cell death characterized by significant iron-dependent peroxidation of phospholipids, is regulated by cellular metabolism, redox homeostasis, and various cancer-related signaling pathways. Recently, considerable progress has been made in demonstrating the critical role of lipid metabolism in regulating ferroptosis, indicating the potential of combinational strategies for treating cancer in the future. In this study, we explored the combinational effects of lipid metabolism compounds and ferroptosis inducers on renal cell carcinoma (RCC) cells. We found potent synergy of the fatty acid amide hydrolase (FAAH) inhibitor URB597 with (1S, 3R)-RSL3 (RSL3) in inhibiting the growth and metastasis of RCC cells bothin vitroandin vivovia induction of G1 cell cycle arrest and promotion of the production of lipid peroxides, malondialdehyde, 4-hydroxynonenal, and cytosolic reactive oxygen species. In addition, inhibition of FAAH increased the sensitivity of RCC cells to ferroptosis. Genome-wide RNA sequencing indicated that the combination of URB597 and RSL3 has more significant effects on regulation of the expression of genes related to cell proliferation, the cell cycle, cell migration and invasion, and ferroptosis than either single agent alone. Moreover, we found that combinational treatment modulated the sensitivity of RCC cells to ferroptosis via the PI3K-AKT signaling pathway. These data demonstrate that dual targeting of FAAH and ferroptosis could be a promising strategy for treating RCC.
Effects of different methods of anesthesia on acute gastrointestinal dysfunction, postoperative cognitive impairment, and the immune status after laparoscopic radical rectal cancer surgery
Authors: He Xue , zhu xiaoquan , Wang Dacheng 
Published: December 2022

Abstract
Background
With an increase in the aged population in China, the annual incidence of rectal cancer is gradually increasing. This study compares the effects of two anesthesia methods on patients undergoing laparoscopic radical rectal cancer surgery.
Method
A total of 230 patients who underwent laparoscopic radical resection for rectal cancer in the Department of Anesthesiology, Huai'an First People's Hospital Affiliated to Nanjing Medical University from 2016 to 2021 were retrospectively analyzed. The general data, clinical symptoms, immune cell count, and cytokine count were collected on the first, third, and fifth postoperative days. In addition, the patients' Mini-Mental State Examination (MMSE) score was recorded, and dynamic analysis was performed to record gastrointestinal dysfunction after the operation.
Results
The postoperative awakening time (18.41 ± 2.88 vs. 23.23 ± 4.34), extubation time (27.84 ± 3.67 vs. 34.32 ± 5.73), and length of hospital stay (9.73 ± 1.32 vs. 12.32 ± 2.34) were shorter, while the postoperative MMSE score (25.43 ± 1.43 vs. 21.32 ± 2.32) was higher in patients administered general anesthesia plus epidural anesthesia than in those administered only general anesthesia. The proportion of CD4 + T lymphocytes was higher (39.45 ± 4.12 vs. 35.45 ± 4.56), and the proportion of CD8 + T lymphocytes was lower (25.34 ± 3.09 vs. 28.43 ± 3.43) in patients undergoing general anesthesia plus epidural anesthesia than in those undergoing only general anesthesia. Moreover, the count of natural killer (NK) cells was lower (0.104 ± 0.021 vs. 0.167 ± 0.024) and the level of human leukocyte antigen–DR isotype (HLA-DR) (66.43 ± 7.43 vs. 56.45 ± 6.43) was higher in patients administered general anesthesia plus epidural anesthesia. In addition, the levels of interleukin (IL)-6, IL-8, and IL-10 decreased with statistical differences (P < 0.05), and the probability of increased intra-abdominal pressure and acute gastrointestinal injury was lower (P < 0.05) in patients undergoing general anesthesia plus epidural anesthesia.
Conclusion
Addition of epidural anesthesia to general anesthesia can reduce the incidence of postoperative cognitive dysfunction, gastrointestinal injury, immunosuppression, postoperative awakening time, extubation time, and length of postoperative hospital stay.
Blast loads and variability on cylindrical shells under different charge orientations
Authors: Yin Fei , Zhi Xudong , Fan Feng , Wei Wuchen , Zheng Dianshuang 
Published: December 2022

Abstract
Cylindrical shells are widely used in public buildings and military protection fields, and it has a high risk of terrorist attacks and military attacks, it is of great social benefit to carry out the anti-blast design of cylindrical shells, which needs to consider building shape and the shape of blast waves. In this paper, cylindrical charges in 5 directions were detonated on the outer ground of the scaled cylindrical shell, blast loads of the cylindrical shell were measured and blast waves were photographed. The variation of blast load is analyzed by combining the test and simulation results, the difference in peak overpressure of the blast waves on the end face between five orientations is nearly twice. The blast loads in the axial direction of cylindrical charges have a secondary peak phenomenon, and the blast loads between the axial direction and radial direction of cylindrical charges change abruptly at a specific angle. The experimental and simulation methods provide a reference for establishing a blast load database of typical buildings.
Functional investigation of five R2R3-MYB transcription factors associated with wood development in Eucalyptus using DAP-seq-ML
Authors: Takawira Lazarus Tichararama , Bachir Ines Hadj , Ployet Raphael , Tulloch Jade , Clemente Helene San , Christie Nanette , Ladouce Nathalie , Dupas Annabelle , Grima-Pettenati Jacqueline , Myburg Alexander A , Mizrachi Eshchar , Mounet Fabien , Hussey Steven Grant 
Published: December 2022

Abstract
A multi-tiered transcriptional network regulates xylem differentiation and secondary cell wall (SCW) formation in plants, with evidence of both conserved and lineage-specific SCW network architecture. We aimed to elucidate the roles of selected R2R3-MYB transcription factors (TFs) linked to Eucalyptus wood formation by identifying genome-wide TF binding sites and direct target genes through an improved DAP-seq protocol combined with machine learning for target gene assignment (DAP-seq-ML). We applied this to five TFs including a well-studied SCW master regulator (EgrMYB2; homolog of AtMYB83), a repressor of lignification (EgrMYB1; homolog of AtMYB4), a TF affecting SCW thickness and vessel density (EgrMYB137; homolog of PtrMYB074) and two TFs with unclear roles in SCW regulation (EgrMYB135 and EgrMYB122). Each DAP-seq TF peak set (average 12,613 peaks) was enriched for canonical R2R3-MYB binding motifs. To improve the reliability of target gene assignment to peaks, a random forest classifier was developed from ArabidopsisDAP-seq, RNA-seq, chromatin, and conserved noncoding sequence data which demonstrated significantly higher precision and recall to the baseline method of assigning genes to proximal peaks. EgrMYB1, EgrMYB2 and EgrMYB137 predicted targets showed clear enrichment for SCW-related biological processes. As validation, EgrMYB137 overexpression in transgenic Eucalyptus hairy roots increased xylem lignification, while its dominant repression in transgenic Arabidopsis and Populus reduced xylem lignification, stunted growth, and caused downregulation of SCW genes. EgrMYB137 targets overlapped significantly with those of EgrMYB2, suggesting partial functional redundancy. Our results show that DAP-seq-ML identified biologically relevant R2R3-MYB targets supported by the finding that EgrMYB137 promotes SCW lignification in planta.
Equid herpesvirus type 3 (EHV-3) synthesizes membrane-associated and secreted forms of glycoprotein G which are not required for efficient virus cell-to-cell in vitro spread
Authors: Losinno Antonella , Vissani María Aldana , Sanchez Diego , Damiani Armando 
Published: December 2022

Abstract
The equid alphaherpesvirus type 3 (EHV-3) ORF 70 gene encodes glycoprotein G (gG), which is conserved in the majority of alphaherpesviruses. This gG is located in the virus envelope with the characteristic of being secreted into the culture medium after proteolytic processing. Alphaherpesviruses’s gG modulate the host antiviral immune response by interacting with chemokines. The aim of this study is to identify and characterize EHV-3 gG. By constructing gG-tagged versions of the virus, gG was detectable in lysates of infected cells, its supernatants and purified virions. A 100-, 60-, and 17-kDa protein forms were detected in viral particles while a 60-kDa form was identified in supernatants of infected cells. The role of EHV-3 gG in the viral infection cycle was assessed by the construction of a gG-minus EHV-3 mutant and its gG-positive revertant. When growth characteristics in equine dermal fibroblast cell line were compared, the plaque size and the growth kinetics of the gG-minus mutant were similar to the revertant virus indicating that EHV-3 gG has not a role neither in direct cell-to-cell transmission nor virus proliferation of EHV-3 in tissue culture. The identification and characterization of EHV-3 gG presented here provides a solid background for further studies to assess whether it has a function in modulating the host immune response.
The Effect of Implant Surgical Experience on the Learning Curve of a Dynamic Navigation System: An In Vitro Study
Authors: Xu Zonghe , Zhou Lin , Zheng Ming , Lin Yanjun , Huang Wenxiu , Chen Jiang , Li Yan , Wu Dong 
Published: December 2022

Abstract
Background
Dynamic navigation systems have a broad application prospect in digital implanting field. This study aimed to explore and compare the dynamic navigation system learning curve of dentists with different implant surgical experience through dental models.
Methods
The nine participants were divided equally into three groups. Group 1(G1) and Group 2(G2) were dentists who had more than 5 years of implant surgical experience. G1 also had more than 3 years of experience with dynamic navigation, while G2 had no experience with dynamic navigation. Group 3(G3) consisted of dentists with no implant surgical experience and no experience with dynamic navigation. Each participant sequentially placed two implants (31 and 36) on dental models according to four practice courses (1–3, 4–6, 7–9, 10–12 exercises). The preparation time, surgery time and related implant accuracy were analyzed.
Results
Three groups placed 216 implants in four practice courses. The regressions for preparation time (F = 10.294, R2 = 0. 284), coronal deviation (F = 4.117, R2 = 0. 071), apical deviation (F = 13.016, R2 = 0. 194) and axial deviation (F = 30.736, R2 = 0.363) were statistically significant in G2. The regressions for preparation time (F = 9.544, R2 = 0. 269), surgery time (F = 45.032, R2 = 0.455), apical deviation (F = 4.295, R2 = 0. 074) and axial deviation (F = 21.656, R2 = 0.286) were statistically significant in G3. Regarding preparation and surgery time, differences were found between G1 and G3, G2 and G3. Regarding implant accuracy, differences were found in the first two practice courses between G1 and G3.
Conclusions
The operation process of dynamic navigation system is relatively simple and easy to use. There is a dynamic navigation learning curve for dentists with or without implant experience. However, dentists with implant experience learn more efficiently and have a shorter learning curve.