bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of maternal complications and neonatal outcome in dichorionic diamniotic twins with fetal weight discordancy measured by ultrasonography
Authors: Kim Hyun Mi , Cha Hyun-Hwa , Seong Won Joon , Lee Hye Jin , Kim Mi Ju 
Published: September 2022

AbstractThis study aimed to determine the relationship between estimated fetal weight discordance by ultrasonography and maternal and neonatal outcomes in dichorionic diamniotic twin pregnancies. We conducted a retrospective review of the medical records of 106 twin pregnancies delivered at a single tertiary center between January 2011 and February 2020. At 20–24 and 28–32 weeks of gestation, participants were divided into two groups: discordant twins with an estimated fetal weight difference of more than 20% and concordant twins with a weight difference of less than 20%. Maternal complications and neonatal outcomes were compared between the two groups. Although the incidences of preeclampsia and placenta previa were significantly higher in discordant twins measured between 20 and 24 weeks, no statistical significance was found in neonatal outcomes. Delivery times were earlier, and neonatal weights were lower in discordant twins measured between 28 and 32 weeks. Neonatal outcomes such as ventilator use and neurodevelopment were also significantly different. Discordance in estimated fetal weight measured using ultrasonography between 20 and 24 weeks can be a risk factor for maternal preeclampsia and placenta previa, whereas discordance at 28–32 weeks may predict poor neonatal outcomes.
The synthesis of novel thioderivative chalcones and their influence on NF-κB, STAT3 and NRF2 signaling pathways in colorectal cancer cells
Authors: Papierska Katarzyna , Krajka-Kuźniak Violetta , Kleszcz Robert , Stefański Tomasz , Kurczab Rafał , Kubicki Maciej 
Published: September 2022

AbstractThis study aimed to synthesize new thioderivative chalcones and analyze their impact on the NF-κB, STAT3, EGFR and Nrf2 signaling pathways in colorectal cancer cells. Among the studied compounds, derivatives 4 and 5 decreased the activation of NF-κB and the expression of the target gene COX-2. In the case of STAT3, we observed the inhibition of activation of this signaling pathway after influencing derivative 4. Increased activation of the Nrf2 signaling pathway was demonstrated for derivatives 5 and 7 in DLD-1 and HCT116 cells. The results of this study indicated that new chalcone derivatives, especially compounds 4, 5, and—to some degree—7, possess potential applications in the prevention of colorectal cancer.
Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker
Authors: He Niqing , Huang Fenghuang , Yu Mingxiang , Zhu Yebao , Li Qingshun Q. , Yang Dewei 
Published: September 2022

AbstractRice blast is a detrimental rice disease caused by the fungusMagnaporthe oryzae. Here, we identified a resistance gene from the rice cultivar Fuhui 2663 which is resistant to the rice blast isolate KJ201. Through isolated population analyses and sequencing approaches, the candidate gene was traced to chromosome 12. With the use of a map-based cloning strategy, the resistance gene was ultimately mapped to an 80-kb resistance locus region containing thePitagene. Candidate gene prediction and cDNA sequencing indicated that the target resistance gene in Fuhui 2663 was allelic toPita, thus being referred to asPita-Fuhui2663hereafter. Further analysis showed that the Fuhui 2663 protein had one amino acid change: Ala (A) residue 918 in Pita-Fuhui2663 was replaced by Ser (S) in Pita-S, leading to a significant change in the 3D structure of the Pita-S protein. CRISPR/Cas9 knockout experiments confirmed thatPita-Fuhui2663is responsible for the resistance phenotype of Fuhui 2663. Importantly,Pita-Fuhui2663did not affect the main agronomic traits of the variety compared to thePitagene as verified by knockout experiments, indicative of potential applications ofPita-Fuhui2663in broader breeding programs. Furthermore, aPita-Fuhui2663-dCAPSmolecular marker with good specificity and high efficiency was developed to facilitate rice breeding for resistance to this devastating disease.
A diselenobis-functionalized magnetic catalyst based on iron oxide/silica nanoparticles suggested for amidation reactions
Authors: Taheri-Ledari Reza , Qazi Fateme Sadat , Saeidirad Mahdi , Maleki Ali 
Published: September 2022

AbstractIn this study, a new heterogeneous magnetic catalytic system based on selenium-functionalized iron oxide nanoparticles is presented and suggested for facilitating amide/peptide bonds formation. The prepared nanocatalyst, entitled as “Fe3O4/SiO2-DSBA” (DSBA stands for 2,2′-diselanediylbis benzamide), has been precisely characterized for identifying its physicochemical properties. As the most brilliant point, the catalytic performance of the designed system can be mentioned, where only a small amount of Fe3O4/SiO2-DSBA (0.25 mol%) has resulted in 89% reaction yield, under a mild condition. Also, given high importance of green chemistry, convenient catalyst particles separation from the reaction medium through its paramagnetic property (ca. 30 emu·g−1) should be noticed. This particular property provided a substantial opportunity to recover the catalyst particles and successfully reuse them for at least three successive times. Moreover, due to showing other excellences, such as economic benefits and nontoxicity, the presented catalytic system is recommended to be scaled up and exploited in the industrial applications.
Dietary 7-ketocholesterol exacerbates myocardial ischemia–reperfusion injury in mice through monocyte/macrophage-mediated inflammation
Authors: Uchikawa Tomoki , Matoba Tetsuya , Kawahara Takuro , Baba Isashi , Katsuki Shunsuke , Koga Jun-ichiro , Hashimoto Yu , Yamasaki Ryo , Ichi Ikuyo , Akita Hidetaka , Tsutsui Hiroyuki 
Published: September 2022

AbstractEmerging evidence suggests that 7-ketocholesterol (7-KC), one of the most abundant dietary oxysterols, causes inflammation and cardiovascular diseases. Here we show the deteriorating effects of dietary 7-KC on myocardial ischemia–reperfusion (IR) injury and detailed the molecular mechanisms. A high-fat high-cholesterol diet containing 7-KC (7KWD) for 3 weeks increased the plasma 7-KC level compared with high-fat high-cholesterol diet in mice. In wild-type mice but not in CCR2−/− mice, dietary 7-KC increased the myocardial infarct size after IR. Flow cytometry revealed that the ratio of Ly-6Chigh inflammatory monocytes to total monocytes was increased in the 7KWD group. Unbiased RNA sequencing using murine primary macrophages revealed that 7-KC regulated the expression of transcripts related to inflammation and cholesterol biosynthesis. We further validated that in vitro, 7-KC induced endoplasmic reticulum stress, mitochondrial reactive oxygen species production, and nuclear factor-kappa B activation, which are associated with increased mRNA levels of proinflammatory cytokines. Administration of N-acetyl-l-cysteine or siRNA-mediated knockdown of PKR-like endoplasmic reticulum kinase or endoplasmic reticulum oxidase 1α suppressed the levels of 7-KC-induced inflammation. Dietary 7-KC exacerbates myocardial IR injury through monocyte/macrophage-mediated inflammation. Endoplasmic reticulum stress and oxidative stress are involved in the 7-KC-induced proinflammatory response in macrophages.
Effect of MA01 rhamnolipid on cell viability and expression of quorum-sensing (QS) genes involved in biofilm formation by methicillin-resistant Staphylococcus aureus
Authors: Saadati Fatemeh , Shahryari Shahab , Sani Naeema Mohseni , Farajzadeh Davoud , Zahiri Hossein Shahbani , Vali Hojatollah , Noghabi Kambiz Akbari 
Published: September 2022

AbstractA group of biosurfactants, called rhamnolipids, have been shown to have antibacterial and antibiofilm activity against multidrug-resistant bacteria. Here, we examined the effect of rhamnolipid biosurfactants extracted from Pseudomonas aeruginosa MA01 on cell growth/viability, biofilm formation, and membrane permeability of methicillin-resistant Staphylococcus aureus (MRSA) ATCC6538 bacterial cells. The results obtained from flow cytometry analysis showed that by increasing the concentration of rhamnolipid from 30 to 120 mg/mL, the cell viability decreased by about 70%, and the cell membrane permeability increased by approximately 20%. In fact, increasing rhamnolipid concentration was directly related to cell membrane permeability and inversely related to cell survival. Microtiter plate biofilm assay and laser scanning confocal microscopy analysis revealed that rhamnolipid, at a concentration of 60 mg/mL, exerts a reducing effect on the biofilm formation of Staphylococcus aureus. Real-time PCR analysis for monitoring the relative changes in the expression of agrA, agrC, icaA, and icaD genes involved in biofilm formation and related to the quorum-sensing pathway after treatment with rhamnolipid indicated a reduced expression level of these genes, as well as sortase A gene. The results of the present study deepen our knowledge regarding the use of microbial natural products as promising candidates for therapeutic applications.
Study on natural frequency response characteristics of coal vibration excited by simple harmonic wave
Authors: Wen Zhihui , Zhang Libo , Wei Jianping , Wang Jianwei , Zhang Junzhao , Jia Yannan , Ren Yongjie 
Published: September 2022

AbstractThe natural frequency of coal is one of the important technical parameters for the application of the permeability enhancement technology of coal and rock forced vibration. Aiming at exploring the dominant frequency of the permeability enhancement technology of coal vibration excited by vibration wave, the model of coal vibration excited by simple harmonic wave (SHW) was constructed. Furthermore, considering the three main control parameters, i.e., excitation force, coal sample size and mechanical parameters, the response characteristics of coal vibration excited by SHW were simulated and calculated. The calculation results demonstrate that when the frequency of excitation force equals the natural frequency of coal, the vibration occurs and the peak values of response parameters all increase significantly. The peak acceleration response of coal increases with the increase of excitation force, whereas it decreases with the increase of coal size. Under the same SHW excitation force, the mechanical parameters of coal determine the vibration response characteristics of coal, and the natural frequency of coal is proportional to the elastic modulus. Finally, the variation law of natural frequency response characteristics of coal vibration excited by SHW was verified by the response experiment on coal vibration under SHW excitation and related test results. The research results can serve as a theoretical basis for the application of the permeability enhancement technology of coal vibration excited by vibration wave.
Construct a classification decision tree model to select the optimal equation for estimating glomerular filtration rate and estimate it more accurately
Authors: Fan Zhenliang , Yang Qiaorui , Xu Zhuohan , Sun Ke , Yang Mengfan , Yin Riping , Zhao Dongxue , Fan Junfen , Ma Hongzhen , Shen Yiwei , Xia Hong 
Published: September 2022

AbstractChronic kidney disease (CKD) has become a worldwide public health problem and accurate assessment of renal function in CKD patients is important for the treatment. Although the glomerular filtration rate (GFR) can accurately evaluate the renal function, the procedure of measurement is complicated. Therefore, endogenous markers are often chosen to estimate GFR indirectly. However, the accuracy of the equations for estimating GFR is not optimistic. To estimate GFR more precisely, we constructed a classification decision tree model to select the most befitting GFR estimation equation for CKD patients. By searching the HIS system of the First Affiliated Hospital of Zhejiang Chinese Medicine University for all CKD patients who visited the hospital from December 1, 2018 to December 1, 2021 and underwent Gate’s method of 99mTc-DTPA renal dynamic imaging to detect GFR, we eventually collected 518 eligible subjects, who were randomly divided into a training set (70%, 362) and a test set (30%, 156). Then, we used the training set data to build a classification decision tree model that would choose the most accurate equation from the four equations of BIS-2, CKD-EPI(CysC), CKD-EPI(Cr-CysC) and Ruijin, and the equation was selected by the model to estimate GFR. Next, we utilized the test set data to verify our tree model, and compared the GFR estimated by the tree model with other 13 equations. Root Mean Square Error (RMSE), Mean Absolute Error (MAE) and Bland–Altman plot were used to evaluate the accuracy of the estimates by different methods. A classification decision tree model, including BSA, BMI, 24-hour Urine protein quantity, diabetic nephropathy, age and RASi, was eventually retrieved. In the test set, the RMSE and MAE of GFR estimated by the classification decision tree model were 12.2 and 8.5 respectively, which were lower than other GFR estimation equations. According to Bland–Altman plot of patients in the test set, the eGFR was calculated based on this model and had the smallest degree of variation. We applied the classification decision tree model to select an appropriate GFR estimation equation for CKD patients, and the final GFR estimation was based on the model selection results, which provided us with greater accuracy in GFR estimation.
Clinical report and predictors of sequelae of 319 cases of pediatric bacterial osteomyelitis
Authors: Krzysztofiak Andrzej , Roversi Marco , Musolino Antonio , Cirillo Marco , Toniolo Renato Maria , Mazza Osvaldo , Gargiullo Livia , Lancella Laura , Rossi Paolo , Villani Alberto ,   Collaborative Osteomyelitis Study Group, Barbuti Domenico , Bernardi Stefania , Bernaschi Paola , Biagiarelli Francesco , Boccuzzi Elena , Bozzola Elena , Carducci Francesca Ippolita Calò , Chiurchiù Sara , Crostelli Marco , Cursi Laura , De Luca Maia , Di Giuseppe Martina , De Benedetti Fabrizio , Deriu Daniele , Giordano Marco , Grandin Annalisa , Insalaco Antonella , Inzaghi Elena , Krzysztofiak Andrzej , Marchesi Alessandra , Marchili Maria Rosaria , Mirra Gianluca , Musolino Antonio , Natali Gian Luigi , Pansini Valeria , Pezzoli Massimo Fabio , Romani Lorenza , Aufiero Lelia Rotondi , Roversi Marco , De Iacobis Isabella Tarissi , Vittucci Anna Chiara 
Published: September 2022

AbstractPediatric osteomyelitis is an insidious disease that can lead to permanent sequelae, the management of which still relies on lengthy intravenous antibiotic therapy. The purpose of this study is to report and describe the clinical course and outcome of pediatric bacterial osteomyelitis in our experience. We reported the clinical, diagnostic, and treatment characteristics of all cases of osteomyelitis in children younger than 18 years of age who were hospitalized between January 2010 and December 2021 at the Bambino Gesù Children’s Hospital in Rome, Italy, we compared patients with and without complications at follow-up, to identify any predictive factor for sequelae. The study sample included 319 cases of pediatric bacterial osteomyelitis. The median age was 7.77 years. Males (60.8%) were more affected than females. The most affected bones were the femur, tibia, and spine. Etiology was identified in 40.1% of cases, with S.aureus as the most common causative agent. Sequelae were reported in 43 cases (13.5%). The main predictors of sequelae were sepsis on admission and hypergammaglobulinemia. Our results show that a severe presentation with sepsis and hypergammaglobulinemia on admission may be associated with a higher frequency of late sequelae. Early recognition and aggressive treatment of this subgroup of patients may lead to a reduction in complications.
3D convolutional neural network for machining feature recognition with gradient-based visual explanations from 3D CAD models
Authors: Lee Jinwon , Lee Hyunoh , Mun Duhwan 
Published: September 2022

AbstractIn the manufacturing industry, all things related to a product manufactured are generated and managed with a three-dimensional (3D) computer-aided design (CAD) system. CAD models created in a 3D CAD system are represented as geometric and topological information for exchange between different CAD systems. Although 3D CAD models are easy to use for product design, it is not suitable for direct use in manufacturing since information on machining features is absent. This study proposes a novel deep learning model to recognize machining features from a 3D CAD model and detect feature areas using gradient-weighted class activation mapping (Grad-CAM). To train the deep learning networks, we construct a dataset consisting of single and multi-feature. Our networks comprised of 12 layers classified the machining features with high accuracy of 98.81% on generated datasets. In addition, we estimated the area of the machining feature by applying Grad-CAM to the trained model. The deep learning model for machining feature recognition can be utilized in various fields such as 3D model simplification, computer-aided engineering, mechanical part retrieval, and assembly component identification.
Differential pharmacological and sex-specific effects of antimuscarinic agents at the hypoglossal motor nucleus in vivo in rats
Authors: Niakani Sepehr , Liu Hattie , Liu Wen-Ying , Horner Richard L. 
Published: September 2022

AbstractSuccessful cholinergic-noradrenergic pharmacotherapy for obstructive sleep apnea (OSA) is thought to be due to effects at the hypoglossal motor nucleus (HMN). Clinical efficacy varies with muscarinic-receptor (MR) subtype affinities. We hypothesized that oxybutynin (cholinergic agent in successful OSA pharmacotherapy) is an effective MR antagonist at the HMN and characterized its efficacy with other antagonists. We recorded tongue muscle activity of isoflurane anesthetized rats (121 males and 60 females, 7–13 per group across 13 protocols) in response to HMN microperfusion with MR antagonists with and without: (i) eserine-induced increased endogenous acetylcholine at the HMN and (ii) muscarine. Eserine-induced increased acetylcholine decreased tongue motor activity (p < 0.001) with lesser cholinergic suppression in females versus males (p = 0.017). Motor suppression was significantly attenuated by the MR antagonists atropine, oxybutynin, and omadacycline (MR2 antagonist), each p < 0.001, with similar residual activity between agents (p ≥ 0.089) suggesting similar efficacy at the HMN. Sex differences remained with atropine and oxybutynin (p < 0.001 to 0.05) but not omadacycline (p = 0.722). Muscarine at the HMN also decreased motor activity (p < 0.001) but this was not sex-specific (p = 0.849). These findings have translational relevance to antimuscarinic agents in OSA pharmacotherapy and understanding potential sex differences in HMN suppression with increased endogenous acetylcholine related to sparing nicotinic excitation.
Assessing related factors to fasting blood sugar and glycosylated hemoglobin in patients with type 2 diabetes simultaneously by a multivariate longitudinal marginal model
Authors: Hosseinzadeh Samaneh , Khatirnamani Zahra , Bakhshi Enayatollah , Heidari Alireza , Naghipour Arash 
Published: September 2022

AbstractThe multivariate marginal model can be used to simultaneously examine the factors affecting both FBS and HbA1c using longitudinal data. The model fitted to multivariate longitudinal data should prevent redundant parameter estimation in order to have greater efficiency. In this study, a multivariate marginal model is used to simultaneously investigate the factors affecting both FBS and HbA1c with longitudinal data for patients with type 2 diabetes in Northern Iran. The present research is a retrospective cohort study. Overall, 500 medical records with complete information were reviewed. The multivariate marginal model is used to determine the factors associated with FBS and HbA1c using longitudinal data. Data have been analyzed in R-3.4.0 using ‘mmm2’ package. Given that the coefficients for the interactions of rtype with the intercept, time, family history of diabetes, history of hypertension, history of smoking, insulin therapy, systolic/diastolic blood pressure and duration of disease at first visit are significantly different from zero (P < 0.05), the effect of the independent variables on the two response variables is different and different coefficients should be used for each. Therefore, the interactions of these variables with rtype are kept in the final model. The coefficients for the interactions of rtype with sex, age at first visit, history of high cholesterol, and weight are not significantly different from zero (P > 0.05), indicating that their effect on the two response variables is similar and only one coefficient should be used for each. We examined the similarity of coefficients when fitting the longitudinal multivariate model for the relationship between FBS/HbA1c and sex, age, history of high blood cholesterol, and body weight. If an independent variable has similar effects on both responses, only one coefficient should be estimated, which will increase the efficiency of the model and the reliability of the results.
Humanized liver TK-NOG mice with functional deletion of hepatic murine cytochrome P450s as a model for studying human drug metabolism
Authors: Uehara Shotaro , Iida Yuichi , Ida-Tanaka Miyuki , Goto Motohito , Kawai Kenji , Yamamoto Masafumi , Higuchi Yuichiro , Ito Satoshi , Takahashi Riichi , Kamimura Hidetaka , Ito Mamoru , Yamazaki Hiroshi , Oshimura Mitsuo , Kazuki Yasuhiro , Suemizu Hiroshi 
Published: September 2022

AbstractChimeric TK-NOG mice with a humanized liver (normal Hu-liver) are a unique animal model for predicting drug metabolism in humans. However, residual mouse hepatocytes occasionally prevent the precise evaluation of human drug metabolism. Herein, we developed a novel humanized liver TK-NOG mouse with a conditional knockout of liver-specific cytochrome P450 oxidoreductase (POR cKO Hu-liver). Immunohistochemical analysis revealed only a few POR-expressing cells around the portal vein in POR cKO mouse livers. NADPH-cytochrome c reductase and cytochrome P450 (P450)-mediated drug oxidation activity in liver microsomes from POR cKO mice was negligible. After the intravenous administration of S-warfarin, high circulating and urinary levels of S-7-hydroxywarfarin (a major human metabolite) were observed in POR cKO Hu-liver mice. Notably, the circulating and urinary levels of S-4′-hydroxywarfarin (a major warfarin metabolite in mice) were much lower in POR cKO Hu-liver mice than in normal Hu-liver mice. POR cKO Hu-liver mice with minimal interference from mouse hepatic P450 oxidation activity are a valuable model for predicting human drug metabolism.