bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: A Retrospective Study of Prevalence of Porcine Parvoviruses 6 in Guangxi, China and Its Co-Infection with Porcine Circovirus
Authors: Yu Ning , Yu Ziping , Chen Luer , Liu Yumeng , Feng Sheng , Zhuang Xinyu , Li Zhuoxin , Li Chenghui , Zhu Xiangyu , Zhang He , Wang Wei , Sun Wenchao , Jin Ningyi , Lu Huijin 
Published: March 2022

Abstract

Background
Parvovirus can infect human and a variety of animals (such as pigs, dogs, cats, etc.). Porcine parvovirus is characterized by infertility, mummified fetuses, early embryonic death, and stillbirths. Worldwide, many porcine parvoviruses (PPVs) have been linked to porcine circovirus (PCV) associated disease (PCVAD).
Results
By PCR amplification and sequencing, the total PPV6-positive rate was 12.5% (62/497) and the positive rate of PPV6 ranged from 12.0–17.6% in differently city. Meanwhile, we found that the positive rate of PPV6 co-infection with PCV (69.4%) was significantly higher than that of PPV6 infection alone (30.6%) (p < 0.01). The positive rate of PPV6 co-infected with PCV2 was significantly higher than that of PPV6 co-infection with PCV3 and PCV4. Of ten PPV6 isolates, six isolates were 6112 nt in length and four isolates were 6111 nucleotides (nt) in length. Compared with the reference strains, we found that the 3' UTRs of ten strains are lacked 14 and 15 bases, respectively, which leads to significant changes in the RNA secondary structure. Within the VP1 unique region, compared with PPV1, PPV2 and PPV3, the motifs of the Ca2+ binding loop (YTGPR) and the catalytic residues (HDIRY) of putative secretory PLA2 were very conserved without amino acid mutation. Phylogenetic analysis of the NS1 gene showed that all PPV6 isolates were located in the genus Copiparvovirus together with PPV4 and PPV5. Phylogenetic analysis of the VP1 gene showed that all PPV6 strains isolated from China clustered in group B, except for the three Chinese strains that clustered in group A. Combined with the results of multiple sequence alignment and genetic evolution, we found a total of 14 amino acid mutation related to phylogenetic tree branches (R14K/Q, I116L/K/T, N788S, R789/M, S/T791H, M792H, A815S, D873G, N919S, R938K, S941T, R1013H, T1038N, T1042D/Y). Compared with PPV6 sequences in different geographic regions, the VP1 gene is more prone to mutation than NS1 gene. From which, two adaptive mutations were found in NS1 (Q3H, P20H) and VP1 (A619T, Q730E), respectively. In addition, we predicted the B cell and T lymphoid epitopes of the NS1 and VP1 genes of 10 PPV6 strains, respectively, and speculated that compare with VP1, the NS1 gene is more suitable as an epitope vaccine target.
Conclusion
In summary, our findings provide new insight into the prevalence of PPV6 in Guangxi, China, as well as lay the foundation for the study of virus infection mechanism.
Urinary Metabolic modulation in human participants residing in Siachen base camp: A 1H NMR Metabolomics Approach
Authors: Gandhi Sonia , Bhadra Kuntal , Gupta Isha , Kanwar Ratnesh Singh 
Published: March 2022

Abstract
The main physiological challenge in high altitude environment is hypoxia which affects the aerobic metabolism reducing the energy supply. These changes may further progress towards extreme environment related diseases. These are further reflected in changes in small molecule metabolites and metabolic pathways. In the present study, metabolic changes due to chronic environmental hypoxia were assessed using 1H NMR metabolomics by comparing the urinary metabolic profile of 70 control soldiers residing at sea level with 40 soldiers residing in Siachen base camp (3700 m) for one year. Multivariate statistical analysis was carried out and PLSDA was able to detect 15 metabolites based on VIP score > 1. ROC analysis was able to detect cis-aconitate, Nicotinamide Mononucleotide, Tyrosine, Choline and Creatinine metabolites with high range of sensitivity and specificity. Pathway analysis revealed 16 pathways impact > 0.05 and phenylalanine tyrosine and tryptophan biosynthesis was the most prominent altered pathway indicating metabolic remodeling to meet the energy requirements. TCA cycle, Glycine serine and Theronine metabolism, Glutathione metabolism and Cysteine alterations were other metabolic pathways which were affected during long-term high-altitude hypoxia exposure. Present findings will help in unlocking new dimension for potential application of NMR metabolomics to address extreme environment related health problems, early detection and developing strategies to combat high altitude hypoxia.
Analysis of PPI networks of transcriptomic expression identifies hub genes associated with Newcastle disease virus persistent infection in bladder cancer
Authors: Ahmad Umar , Abdullah Syahril , Chau De Ming , Chia Suet Lin , Yusoff Khatijah , Chan Soon Choy , Ong Teng Aik , Razack Azad Hassan , Veerakumarasivam Abhi 
Published: March 2022

Abstract
Bladder cancer cells acquire persistent infection associated with oncolytic Newcastle disease virus (NDV) in which its molecular events are still unclear. This poses a potential problem for oncolytic virus application for cancer therapy. To unravel the molecular mechanism underlying the development of NDV persistent infection in bladder cancer, we used mRNA expression profile of the persistently infected bladder cancer cells to construct PPI network. Based on path and module exploring in the PPI network, the bridges were found mainly from pathways of p53 signalling, ECM-receptor interaction, and TGF-beta signalling by the upregulated mRNAs, to the antigen processing and presentation, protein processing in endoplasmic reticulum, completement and coagulation cascades by the downregulated mRNAs in NDV persistent TCCSUPPi cells. In persistent EJ28Pi cells comparatively, connections were identified mainly from pathways of renal carcinoma, viral carcinogenesis, Ras signalling and cell cycle by the upregulated mRNAs, to the Wnt signalling, HTLV-I infection and pathways in cancer by the downregulated mRNAs. This connection was mainly dependent on of RPL8- HSPA1A/HSPA4 in TCCSUPPi cells and EP300, PTPN11, RAC1 - TP53, SP1, CCND1 and XPO1 in EJ28Pi cells. Oncomine validation showed that the top hub genes identified in the network that includes RPL8, THBS1, F2 from TCCSUPPi and TP53 and RAC1 from EJ28Pi are involved in the development and progression of bladder cancer. Protein-drug interaction network, have identified several drugs targets that could be used to disconnect the linkages between modules and prevent bladder cancer cells from acquiring NDV persistent infection. This is the first time reporting the PPI network analysis of differentially expressed mRNAs of the NDV persistently infected bladder cancer cell lines which provide an insight into screening drugs that could be used together with NDV to manage bladder cancer resistance to therapy and progression.
Magnified endoscopy with texture and color enhanced imaging with indigo carmine for superficial nonampullary duodenal tumor: A pilot study
Authors: Okimoto Kenichiro , Matsumura Tomoaki , Maruoka Daisuke , Kurosugi Akane , Shiratori Wataru , Nagashima Ariki , Ishikawa Tsubasa , Kaneko Tatsuya , Kanayama Kengo , Akizue Naoki , Ohta Yuki , Taida Takashi , Saito Keiko , Kato Jun , Kato Naoya 
Published: March 2022

Abstract
This pilot study aimed to investigate the utility of texture and color enhancement imaging (TXI) with magnified endoscopy (ME) for the preoperative diagnosis of superficial nonampullary duodenal epithelial tumors (SNADETs). We prospectively evaluated 12 SNADETs. The visibility for ME-TXI, ME with indigo carmine (ICME) - white-light imaging (WLI), ICME-TXI compared to ME-NBI (narrow-band imaging) was scored (+ 2 to − 2 ME-NBI was set as score 0) by 3 experts. Scores + 2 and + 1 were defined as improved visibility. The interobserver agreement for improved visibility of surface structure (SS) was evaluated. Sensitivity, specificity, and positive predictive value (PPV) for Vienna Classification (VCL) C4/5 associated with the preoperative diagnosis of ICME-TXI were analyzed. The SS visibility score of ICME-TXI was significantly higher than that of ME-NBI, ME-TXI, and ICME-WLI (P < 0.001 respectively). The kappa coefficients of reliability for interobserver agreement for the SS visibility improvement with ICME-TXI were 0.70, 0.96, and 0.96. All endoscopists preferred ICME-TXI for visualizing SS mostly for all lesions. The sensitivity, specificity, and PPV (%) of ICME-TXI for VCL C4/5 were 80, 66.7, and 63.2, respectively. ICME-TXI facilitates the visibility of the SS of SNADETs and may contribute to their preoperative diagnosis.
Calibration of miniature air quality detector monitoring data with PCA–RVM–NAR combination model
Authors: Liu Bing , Zhang Yirui 
Published: March 2022

Abstract
The development of miniature air quality detectors makes it possible for humans to monitor air quality in real time and grid. However, the accuracy of measuring pollutants by miniature air quality detectors needs to be improved. In this paper, the PCA-RVM-NAR combined model is proposed to calibrate the measurement accuracy of the miniature air quality detector. First, correlation analysis is used to find out the main factors affecting pollutant concentrations. Second, principal component analysis is used to reduce the dimensionality of these main factors and extract their main information. Thirdly, taking the extracted principal components as independent variables and the observed values of pollutant concentrations as dependent variables, a PCA-RVM model is established by the relevance vector machine. Finally, the nonlinear autoregressive neural network is used to correct the error and finally complete the establishment of the PCA-RVM-NAR model. Root mean square error, goodness of fit, mean absolute error and relative mean absolute percent error are used to compare the calibration effect of PCA-RVM-NAR model and other commonly used models such as support vector machine, multilayer perceptron neural network and nonlinear autoregressive models with exogenous input. The results show that, no matter which pollutant, the PCA-RVM-NAR model achieves better calibration results than other models in the four indicators. Using this model to correct the data of the miniature air quality detector can improve its accuracy by 77.8%~93.9%.
Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR
Authors: yin Xinying , Li Xin , Yang Lili , Zheng Qiuyue , Yongzhe Piao , Jijuan Cao 
Published: March 2022

Abstract
Background
Seeds are an important medium for long-distance transmission of plant viruses; therefore, appropriate, sensitive methods for detecting low concentrations of virus in seeds are crucial to ensure the quality of seed lots. In the present study, we developed a one-step pre-amplification reverse transcription quantitative PCR (RT-qPCR) assay based on the TaqMan technology to detect Cucumber green mottle mosaic virus (CGMMV) in zucchini seeds.
Result
Seed powder samples with simulated virus infection at a low concentration were prepared (the mass ratio 1:900 and 1:1000), and their uniformity was verified using one-step pre-amplification RT-qPCR. We used one-step pre-amplification RT-qPCR to detect CGMMV in low-concentration virus-infected seeds and compared this method with double antibody sandwich–enzyme-linked immunosorbent assay and universal RT-qPCR, which are the main methods used for virus detection in seeds. The minimum limit of detection (LOD) of the improved one-step pre-amplification RT-qPCR method for simulated CGMMV-infected seeds in a large sample of seeds is 0.1%.
Conclusions
It can reliably and stably detect a single virus-infected seed in 1000 seeds and demonstrates a higher detection sensitivity than universal RT-qPCR and double antibody sandwich–enzyme-linked immunosorbent assay. Our improved one-step pre-amplification RT-qPCR assay has proved to be very suitable for the analysis of large seed lots.
CNTNAP4 partial deficiency exacerbates α -synuclein pathology through astrocyte–microglia interplay
Authors: Zhang Wenlong , Ding Liuyan , Gong Junwei , Zhang Mengran , Ma Runfang , Zheng Shaohui , Zhang Zhiling , Xu Pingyi , Xu Huaxi , Zhang Yunlong 
Published: March 2022

Abstract
BackgroundParkinson’s disease (PD) is a common progressive neurodegenerative movement disorder, which is characterized by dopaminergic (DA) neuron death and the aggregation of neurotoxic α-synuclein. CNTNAP4, a risk gene of autism, has been implicated to participate in DA neuron degeneration in PD pathogenesis. However, the role of CNTNAP4 in α-synuclein pathology remains unclear. MethodsHeterozygous CNTNAP4 null mice injected with adeno-associated viral vector (AAV)-mediated human α-synuclein (AAV-hα-Syn) were used to evaluate the impact of partial CNTNAP4 loss on α-synuclein pathology. The resulting physiological/behavioral effects were assessed using behavioral tests, western blotting, immunohistochemistry, and transmission electron microscopy. The underlying mechanism was studied using RNA-sequencing. Cytokine expression and secretion were examined using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and Magnetic Luminex assay kit.ResultsHere we showed CNTNAP4 partial deficiency exacerbates α-synuclein pathology, nigrostriatal DA neuron degeneration and motor impairment, induced by AAV-hα-Syn overexpression. Mechanistically, aggravated α-synuclein pathology stimulates complement C3 release by astrocytes, activating microglial C3a receptor, which in turn triggers microglia to secrete complement C1q and pro-inflammatory cytokines. The astrocyte–microglia crosstalk further drives DA neuron death and motor dysfunction in PD. Furthermore, we showed that in vivo depletion of microglia rescues the aggravated α-synuclein pathology resulting from partial loss of CNTNAP4. ConclusionsOur results indicate that CNTNAP4 plays a key role in α-synuclein pathogenesis by regulating glial crosstalk and may be a potential target for PD treatment.
Associations Between Digital Media Use and Brain Surface Structural Measures in Preschool-Aged Children
Authors: Hutton John S. , Dudley Jonathan , DeWitt Thomas , Horowitz-Kraus Tzipi 
Published: March 2022

Abstract
The American Academy of Pediatrics recommends limits on digital media use (“screen time”), citing cognitive-behavioral risks. Media use in early childhood is ubiquitous, though few imaging-based studies have been conducted to quantify impacts on brain development. Cortical morphology changes dynamically from infancy through adulthood and is associated with cognitive-behavioral abilities. The current study involved 52 children who completed MRI and cognitive testing at a single visit. The MRI protocol included a high-resolution T1-weighted anatomical scan. The child’s parent completed the ScreenQ composite measure of media use. MRI measures included cortical thickness (CT) and sulcal depth (SD) across the cerebrum. ScreenQ was applied as a predictor of CT and SD in regression analyses, controlling for sex and age. Higher ScreenQ scores were correlated with lower CT in right-sided occipital, parietal and supramarginal areas (p<0.10, family-wise error/FWE-corrected) and also lower SD in inferior temporal/fusiform areas (p<0.05, FWE-corrected). These areas support primary visual and higher-order processing, notably social cognition. Results align with those of lower CT with higher media use recently described in adolescents. While differences in visual areas likely reflect maturation, those in higher-order areas expected to be in an accretive stage at this age suggest under-development, though further studies are needed.
Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling pathway
Authors: Liang Xueyun , Wang Yan , Liu Jiaxin , Yu Baocong , Jin Yiran , Li Jiahui , Ma Xiaona , Yu Jianqiang , Niu Jianguo 
Published: March 2022

Abstract
In this study, we aimed to clarify the protective effect of umbilical cord-derived mesenchymal stem cell conditioned medium (UC-MSC-CM) on neuronal oxidative injury and its potential mechanism. Neuronal oxidative damage was mimicked by H2O2 treatment of the HT22 cell line. Immunofluorescence staining was used to analyze the expression of cleaved Caspase3 and TRPM2. Cytotropic factors secreted by UC-MSCs were analyzed by enzyme-linked immunosorbent assay. Whole-cell patch clamping was used to detect TRPM2-like currents. Western blotting was utilized to analyze the protein levels of SOD, p-ERK1/2, p-JNK1/2/3 and cleaved-Caspase-9. The number of cleaved-Caspase-3-positive cells and protein expression of Caspase-9 induced by H2O2 treatment were decreased by UC-MSC-CM treatment. Furthermore, SOD protein expression was increased in the MSC-CM group compared with that in the H2O2 group. The H2O2-induced TRPM2-like currents in HT22 cells were attenuated by MSC-CM treatment. In addition, H2O2 treatment downregulated the expression of p-JNK protein in HT22 cells, and this the downward trend was reversed by incubation with MSC-CM. Thus, we showed that UC-MSC-CM protects neurons against oxidative injury, possibly by inhibiting activation of TRPM2 and the JNK signaling pathway.
A Study on Dynamic Stress of Planetary Gear Transmission in Non-Inertial System
Authors: Wei Jing , Cao Miaofei , Zhang Aiqiang , Cheng Hao , Peng Bin 
Published: March 2022

Abstract
Considering the comprehensive effect of the internal non-inertial system (INIS) of planetary gear transmission (PGT) and external non-inertial system (ENIS) of the body, combined with the modified Heywood formula and Hertz formula, a calculation model for the dynamic bending stress and contact dynamic stress of the PGT in a non-inertial system (NIS) is proposed. The stress non-inertial coefficients (KNI) describing the influence of the additional effects on the dynamic stress in the NIS are defined. Additionally, the variation law of non-inertial coefficients under two typical non-inertial conditions of variable-speed level flight and somersault motion was studied. The effect of gear installation and manufacturing errors on the non-inertial coefficients under two maneuvering conditions was analyzed. The results indicated that non-inertial coefficients increase with an increase in the maneuverability of the body, and the variable-speed horizontal flight and somersault motion have significant effects on the changing trend of non-inertial coefficients with the gear installation and manufacturing errors. Compared with the gear error, the effect of the NIS has a significant influence on the non-inertial coefficients.
Prognostic value of albumin/lactate dehydrogenase ratio in AIDS-related lymphoma
Authors: hui jiangjin , Wan Zhikai , Lang Guanjing , Zhu Biao 
Published: March 2022

Abstract
Objective
This study aimed to explore the prognostic factors of patients with AIDS-related lymphoma.
Methods
The clinical data of 80 patients with AIDS-related lymphoma were retrospectively analyzed. According to the survival status of the patients, the patients were divided into a death group and a survival group. The factors affecting the prognosis of patients were analyzed by binary logistic regression.
Results
Binary logistic regression analysis showed that central metastasis and albumin/lactate dehydrogenase ratio (ALB/LDH) were the prognostic factors of ARL patients. The results showed that central metastasis (P = 0.033) was a risk factor, and ALB/LDH (P = 0.001) was a protective factor.
Conclusion
The albumin/lactate dehydrogenase ratio is a convenient, practical and inexpensive potential marker that can be used to predict the prognosis of patients with AIDS-related lymphoma and provide guidance for clinical treatment.
Advanced Cu-free Bipolar Solid-state Lithium-ion Battery Promoted by Li+ Conductive Matrix Enabled Excellent Iron Oxide Anode
Authors: Dong Wujie , Zhao Yantao , Cai Mingzhi , Dong Chenlong , Ma Wenqin , Pan Jun , Lv Zhuoran , Dong Hang , Tang Yufeng , Huang Fuqiang 
Published: March 2022

Abstract
Bipolar lithium-ion batteries (b-LIBs) with internal series configuration possess great advantages in conversion rate from theoretical energy density to practical values when compared with classic ones. Herein, advanced Cu-free b-LIBs with further simplified structure can be achieved by utilizing an amorphous Li+ conductive Li-Fe-BO2 matrix enabled iron oxide anode (FeOx-LFBO) with intermediate cutoff voltage (≥0.5 V) beyond lithiation potential of Al foil. The rational designed LFBO matrix can serve as a Li+ conductor/reservoir to enable fast Li+ diffusion, a heterogeneous nano-reactor to interdict aggregation of Fe + Li2O precipitates, a binder to aggregate particles, and a soft buffer to remit volume change. The FeOx-LFBO has a high tap density of 1.6 g/cm3, good electronic conductivity, and high Li+ conductivity. As an anode the intermediate cutoff voltage of 0.5 V, it harvests the admirable capacity of 710 mA h/g at 0.5 A/g, excellent cycling stability (95% after 1000 cycles), and capacitive rate capability (192 mA h/g at 50 A/g). Such an excellent anode is assembled into a novel Cu-free LIB, delivering an ultra-high energy density of 350 Wh/kg, outstanding power density of 6700 W/kg, and long-term cycling stability (75% after 2000 cycles), much better than state-of¬-the-art Li4Ti5O12 battery (≤100 Wh/kg, ≤5000 W/kg). Moreover, a Cu-free solid-state bipolar pouch cell is also fabricated sucessfully with high volatge of 7.6 V, great cycling stability, and safety. This study may set off an upsurge in studying intermediate cut-off voltage anodes and novel b-LIBs in the future.
Polygenic Risk, Lifestyle, and Cardiovascular Mortality: A Prospective UK Biobank Study
Authors: Yun Jae-Seung , Jung Sang-Hyuk , Shivakumar Manu , Xiao Brenda , Khera Amit V. , Park Woong-Yang , Won Hong-Hee , Kim Dokyoon 
Published: March 2022

Abstract
BackgroundPrevious studies primarily targeted the ability of polygenic risk scores (PRSs) to predict a specific disease, and only a few studies have investigated the association between genetic risk scores and cardiovascular (CV) mortality. We assessed PRSs for coronary artery disease (CAD) and type 2 diabetes (T2DM) as predictive factors for CV mortality, independent of traditional risk factors, and further investigated the additive effect between lifestyle behavior and PRS on CV mortality. MethodsWe used genetic and phenotypic data from UK Biobank participants aged 40–69 years at baseline, collected with standardized procedures. Genome-wide PRSs were constructed using >6 million genetic variants. Cox proportional hazard models were used to analyze the relationship between PRS and CV mortality with stratification by age, sex, disease status, and lifestyle behavior. ResultsOf 377,909 UK Biobank participants having European ancestry, 3,210 (0.8%) died due to CV disease during a median follow-up of 8.9 years. CV mortality risk was significantly associated with CAD PRS (low vs. very high genetic risk groups, CAD PRS hazard ratio [HR] 2.61 [2.02–3.36]) and T2DM PRS (HR 2.08 [1.58–2.73]), respectively. These relationships remained significant even after adjustment for a comprehensive range of demographic and clinical factors. In the very high genetic risk group, adherence to an unfavorable lifestyle was further associated with a substantially increased risk of CV mortality (favorable versus unfavorable lifestyle with very high genetic risk for CAD PRS, HR 8.31 [5.12–13.49]; T2DM PRS, HR 5.84 [3.39–10.04]). Across all genetic risk groups, 32.1% of CV mortality was attributable to lifestyle behavior (population attributable fraction [PAF] 32.1% [95% CI 28.8–35.3%]) and 14.1% was attributable to smoking (PAF 14.1% [95% CI 12.4–15.7%]). There was no evidence of significant interaction between PRSs and age, sex, or lifestyle behavior in predicting the risk of CV mortality. ConclusionsPRSs for CAD or T2DM and lifestyle behaviors are independent predictive factors for future CV mortality in the white, middle-aged population. PRS-based risk assessment could be useful to identify individuals who need intensive behavioral or therapeutic interventions to reduce the risk of CV mortality.