bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time required for commitment to T cell proliferation depends on TCR affinity and cytokine response
Authors: Wu Liang‐zhe , Balyan Renu , Brzostek Joanna , Zhao Xiang , Gascoigne Nicholas R J 
Published: November 2022

AbstractT cell activation and effector functions are determined by the affinity of the interaction between T cell receptor (TCR) and its antigenic peptide MHC (pMHC) ligand. A better understanding of the quantitative aspects of TCR‐pMHC affinity‐dependent T cell activation is critical for the development of new immunotherapeutic strategies. However, the role of TCR‐pMHC affinity in regulating the kinetics of CD8+ T cell commitment to proliferation and differentiation is unknown. Here, we show that the stronger the TCR‐pMHC affinity, the shorter the time of T cell‐APC co‐culture required to commit CD8+ T cells to proliferation. The time threshold for T cell cytokine production is much lower than that for cell proliferation. There is a strong correlation between affinity‐dependent differences in AKT phosphorylation and T cell proliferation. The cytokine IL‐15 increases the poor proliferation of T cells stimulated with low affinity pMHC, suggesting that pro‐inflammatory cytokines can override the affinity‐dependent features of T cell proliferation.
Надтверді матеріали для оборонної промисловості та повоєнного розвитку України
Authors: Бочечка Олександр Олександрович 
Published: November 2022

У доповіді наведено найбільш важливі й актуальні результати фундаментальних і прикладних досліджень, проведених в Інституті надтвердих матеріалів ім. В.М. Бакуля НАН України, завдяки яким в Україні створено науково-технічну базу для розроблення сучасних технологій та обладнання з надтвердих матеріалів, що дає можливість значно підвищити якість і продуктивність виготовлення інструментів та деталей, зокрема для військової техніки.
МАТЕМАТИЧНЕ МОДЕЛЮВАННЯ ВПЛИВУ ТЕМПЕРАТУРНОГО РЕЖИМУ НА ЕФЕКТИВНІСТЬ СОНЯЧНОЇ ПАНЕЛІ
Authors: Захаров Д.В. , Книш Л.І. 
Published: November 2022

Побудовано стаціонарну 2D математичну модель, що описує перенос енергії в монокристалічній кремнієвій сонячній панелі. Апроксимовані експериментальні дані температурної залежності коефіцієнта корисної дії сонячної панелі були введені в рівняння як джерельний член. Вплив умов навколишнього середовища враховувався в нелінійних граничних умовах моделі. Розроблено ітераційний числовий алгоритм, на основі якого отримано двовимірне поле температур в кремнієвій сонячній панелі. Проведено порівняння середньої ефективності сонячної панелі для двох випадків: з урахуванням залежності коефіцієнту корисної дії від температури та при постійному значенні коефіцієнта корисної дії. Отримані дані можуть використовуватись при проєктуванні сонячних панелей та оптимізації їх роботи.
PENDAMPINGAN PEMBUATAN KRAN LESS CONTACT UNTUK MENGURANGI PENYEBARAN COVID-19 DI MASJID FATHUL JANNAH
Authors: Supiyati Supiyati , Yuliza Elfi , Ekawita Riska 
Published: November 2022

Fasilitas umum tempat wudhu dan mencuci tangan di Masjid Fathul Jannah masih menggunakan kran biasa, padahal banyak jamaah menggunakan fasilitas mencuci tangan ini, yang dimungkinkan akan menjadi tempat penularan covid-19. Tujuan dari kegiatan ini adalah memberikan edukasi cara penggunaan fasilitas cuci tangan/ wudhu yang tepat pada jamaah untuk mencegah penularan covid-19, memberikan pengetahuan dan keterampilan serta pendampingan pada pengurus masjid Fathul Jannah mengenai teknologi sederhana cara merangkai dan membuat program sistem kran less contact, beserta instalasinya. Metode dalam kegiatan pengabdian pada masyarakat ini adalah pelatihan dan pendampingan. Hasil kegiatan menunjukan bahwa kegiatan pengabdian pada masyarakat dapat dilaksanakan dengan baik dan lancar. Berdasarka free test dan post test yang telah dilakukan terjadi peningkatan pengetahuan para peserta 95%. Peserta dengan didampingi tim pengabdi sudah berhasil merangkai dan memogram dua set kran less contact . Peserta juga telah berhasil memasang instalasi kran less contact di masjid Fathul Jannah dan sudah dapat digunakan oleh jamaah masjid untuk cuci tangan dan wudhu tanpa menyentuh kran, sehingga usaha untuk mengurangi penyebaran covid-19 telah berhasil dilaksanakan.
¿Podríamos atrevernos a desear un mundo mejor y a trabajar en su construcción?
Authors: Martínez Salgado Carolina 
Published: November 2022

La enfermedad es una de las condiciones de existencia de nuestra especie: los humanos no podemos escapar a los límites de nuestro basamento biológico. Pero los problemas de salud con los que la población lidia en cada época de su historia, no surgen de la nada. Tampoco son el resultado del simple desarrollo natural e ineludible de los acontecimientos.
La epidemiología plantea que las enfermedades son el resultado de complejas interacciones entre las características innatas de las personas, genéticamente determinadas, y las condiciones del entorno ambiental en el que transcurre su vida. La epidemiología social ha reunido suficiente evidencia para mostrar que la configuración de los perfiles de daños a la salud de las poblaciones es extremadamente sensible a las características de cada sociedad, y a la ubicación que en ella tienen sus integrantes.
Pero las perspectivas epidemiológicas más críticas nos han ayudado a comprender que no basta con admitir que los problemas de salud de las poblaciones humanas guardan una estrecha relación con el mundo en el que transcurre su vida, para cambiar los graves efectos que en ello tienen las ‘elecciones’ que como civilización hemos hecho. Para lograrlo haría falta algo mucho más radical: movernos, como civilización, en una nueva dirección. Sólo que dilucidar cuál es esa dirección no resulta sencillo. ¿Hacia dónde tendríamos que dirigirnos en la búsqueda de mejores condiciones para la salud?. Una pregunta así no podría responderse sin considerar la abrumadora complejidad del mundo del cual forman parte los fenómenos a cuya comprensión pretendemos acercarnos. Pero tampoco encontraría respuestas posibles si abdicáramos de nuestra capacidad para imaginar otro orden de cosas, para desear un mundo distinto y para trabajar en su construcción.
An Implementation of Online Extensive Reading to Improve Reading Comprehension of Social Work Students
Authors: Pongsatornpipat Warangkana 
Published: November 2022

This research aimed at studying the reading skills of finding the main idea of an article, learning new vocabulary, and students’ opinions towards extensive reading through online weekly journals. The sample consisted of 27 third-year students from the Faculty of Social Administration enrolled in English for Social Workers 1 in the first semester of the 2021 academic year. The research instruments consisted of online weekly articles published in reliable online sources, such as the BBC, CNN, and National Geographic, and were relevant to their field of study. The findings showed that (1) students were able to identify the main idea of the written text from reliable online sources based on a topic of interest or field of study. Students could write a summary of the written text to determine the main idea. As for students’ opinions about extensive reading after eight weeks of online reading activities, (2) students were able to read faster and were familiar with reading strategies during the activities. They were capable of reading long articles with new vocabulary and did not have to rely on translation tools.
Adaptive Modelling System as a Unifi ed Platform for Industry-Specifi c CAD Systems
Authors: Gordin M. V. , Ivanova G. S. , Proletarsky A. V. , Fetisov M. V. 
Published: November 2022

The risks associated with the isolated design of complex software systems within individual industries are analyzed, where not only the same thing is often done, but also the quality of the design suffers due to incomplete competence of the implementers. The approach of dividing competence and responsibility in complex software development by introducing an additional domain-specific layer of interaction between the software developer and the subject area specialists is discussed. The use of an adaptive modelling system as a tool for such separation is proposed. It is shown that the use of adaptive modelling as a common development platform for industry-specific CAD will not only improve the quality of production design in different industries, but will also simplify the design of production in related fields. Finally, it is shown that the use of a common platform will avoid the costs associated with the trend towards simplification and atomization of software developed in our country in the face of sanctions and the degradation of global connections.
Prediction of Thermal Distribution in an Internally Heated Radiative-Convective Moving Porous Fin with variable Thermal Conductivity using Homotopy Perturbation Method
Authors: Oladosu Suraju Aremu , Kuku Rafiu Olalekan , Sobamowo Gbeminiyi Musibau , Siqueira Antonio Marcos de Oliveira 
Published: November 2022

Thermal distribution of a rectangular moving convective-radiative porous fin with temperature-dependent thermal conductivity and internal heat generation is analyzed in this work homotopy perturbation method. With the aid of the analytical solutions, the impacts of the model parameters on the thermal behaviour of the fin are investigated. The parametric analysis reveals that increase in porosity and convective parameters, the rate of heat transfer from the fin increases and consequently improves the efficiency of the fin. The values of the temperature distribution in the fin increase as the Peclet number increases. However, as thermal conductivity and internal heat generation increase, the rate of heat transfer from the fin decreases. Therefore, the operational parameters of the fin must be carefully selected to ensure that the fin retains its primary purpose of removing heat from the primary surface.
Assessment of Posterolateral Lumbar Fusion
Authors: Lehr A. Mechteld , Duits Anneli A.A. , Reijnders Maarten R.L. , Nutzinger Don , Castelein René M. , Oner F. Cumhur , Kruyt Moyo C. 
Published: November 2022

Background:
Noninvasive assessment of osseous fusion after spinal fusion surgery is essential for timely diagnosis of patients with symptomatic pseudarthrosis and for evaluation of the performance of spinal fusion procedures. There is, however, no consensus on the definition and assessment of successful posterolateral fusion (PLF) of the lumbar spine. This systematic review aimed to (1) summarize the criteria used for imaging-based fusion assessment after instrumented PLF and (2) evaluate their diagnostic accuracy and reliability.

Methods:
First, a search of the literature was conducted in November 2018 to identify reproducible criteria for imaging-based fusion assessment after primary instrumented PLF between T10 and S1 in adult patients, and to determine their frequency of use. A second search in July 2021 was directed at primary studies on the diagnostic accuracy (with surgical exploration as the reference) and/or reliability (interobserver and intraobserver agreement) of these criteria. Article selection and data extraction were performed by at least 2 reviewers independently. The methodological quality of validation studies was assessed with the QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies 2) and QAREL (Quality Appraisal of Reliability Studies).

Results:
Of the 187 articles included from the first search, 47% used a classification system and 63% used ≥1 descriptive criterion related to osseous bridging (104 articles), absence of motion (78 articles), and/or absence of static signs of nonunion (39 articles). A great variation in terminology, cutoff values, and assessed anatomical locations was observed. While the use of computed tomography (CT) increased over time, radiographs remained predominant. The second search yielded 11 articles with considerable variation in outcomes and quality concerns. Agreement between imaging-based assessment and surgical exploration with regard to demonstration of fusion ranged between 55% and 80%, while reliability ranged from poor to excellent.

Conclusions:
None of the available criteria for noninvasive assessment of fusion status after instrumented PLF were demonstrated to have both sufficient accuracy and reliability. Further elaboration and validation of a well-defined systematic CT-based assessment method that allows grading of the intertransverse and interfacet fusion mass at each side of each fusion level and includes signs of nonunion is recommended.

Level of Evidence:
Diagnostic Level III. See Instructions for Authors for a complete description of levels of evidence.
The acidity of oxalic acid induces regulated cell death in Arabidopsis
Authors: Huang Guozhong , Liu Xiaotong , Zou Yanmin , Xiao Zejun , Chen Kexing , Cao Nana , Lu Dongping 
Published: November 2022

Abstract
Key message The acidity of oxalic acid (OA) induces an unknown type of cell death in Arabidopsis. Acid treatment and Sclerotinia sclerotiorum infection trigger some overlapping transcriptomic responses. The influx of calcium is likely required for OA-induced cell death in Arabidopsis.

Abstract Numerous pathogens trigger host cell death that either favors or blocks infection. Many phytopathogens produce cytolytic compounds functioning as virulence determinants, like oxalic acid (OA). It was reported that oxalate itself but not the acidity of OA induced programmed cell death in plants. Accumulation of oxalate often reaches millimolar concentrations, and the pH value decreases to around 4.0 in the Sclerotinia sclerotium -infected plant tissues. However, the role of OA acidity in inducing cell death remains unknown. We used the approaches of Sytox green/fluorescein diacetate (FDA)/propidium iodide (PI)/Evan's blue staining, transcriptomic analysis, and dynamic gene expression analysis to investigate the role of OA acidity in inducing cell death and the regulatory mechanisms of OA-induced cell death. We found that the acidity of OA and HCl induced cell death in Arabidopsis with characteristic features including impaired plasma membrane and induction of cell-death related genes, but not DNA fragmentation. Moreover, calcium influx is required for OA-induced cell death. Our work reveals that acids induce an uncharacterized-type of cell death that is a regulated process, instead of a passive event.
N6-methyladenosine-mediated changes in SOCS3 expression regulate skeletal muscle stem cell senescence
Authors: Zhu Menghai , Lian Chong , Chen Gang , Zou Peng , Qin Beng gang 
Published: November 2022

Abstract
N6-methyladenosine (m6A)-associated mechanisms are involved in cellular metabolic activities; however, their role in skeletal muscle stem cell (SkMSC) senescence is unknown. In this study, m6A RNA modification and METTL3 expression were decreased in aged SkMSCs from mice. Transcriptional analysis of m6A modification further revealed that suppressor of cytokine signaling 3 (SOCS3) was downregulated in aged SkMSCs, and that downregulation of METTL3 promoted SkMSC senescence. In addition, the upregulation of METTL3 alleviated the senescence of SkMSCs. Mechanistically, deletion of m6A modifications promoted senescence and inhibited SOCS3 expression, whereas downregulation of SOCS3 promoted senescence. Moreover, insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) was identified as an important regulator in the recognition and stabilization of m6A modified SOCS3 expression. IGF2BP1 binds to SOCS3 and reinforces its stability by suppressing the phosphorylation of JAK2/STAT3. Therefore, METTL3 inhibits SkMSC senescence through m6A modification-dependent stabilization of SOCS3 expression and inhibition of JAK2-STAT3 signaling. This study thus highlights a novel mechanism underlying SkMSC senescence.
Saffron-Fusarium oxysporum R1 dual transcriptomics unravels, defense mechanism of saffron and robust pathogenicity of Fusarium oxysporum R1
Authors: Bhagat Nancy , Mansotra Ritika , Patel Karan , vakhlu jyoti 
Published: November 2022

Abstract
Fusarium oxysporum causes corm rot in saffron (Crocus sativus L.), that is one of the most important fungal diseases impacting saffron yield globally. Despite the fact that the corm rot agent and its symptoms are widely known, little is known about the molecular basis of defense mechanism of saffron in response to Fusarium oxysporum infection. Therefore, the current study was initiated in order to identify differentially expressed genes in response to pathogen infection in saffron. The active participation of Mitogen Activated Kinase pathway (MAPK), Transcription factors (TFs), plant-hormone signalling, plant-pathogen interaction pathway and synthesis of PR proteins in defence of saffron against Fox R1 infection was revealed by Gene Ontology, KEGG pathway and MapMan analysis. In this study, the PR proteins had shown a robust antifungal activity. These findings revealed that the saffron has a powerful defense mechanism in the early stages of infection. In addition, fifty seven Fusarium oxysporum R1 genes linked to pathogenicity and virulence that expressed during the infection phase were also identified. Surprisingly, SIXgenes (secreted in the xylem) were not found in the current investigation, although these genes have been thoroughly described in other Fusarium oxysporum strains and are known to be one of the key virulence factors. Because saffron is a male sterile plant that can only be improved genetically by genome editing, this work will serve as a foundation for identifying genes that can be used to create saffron varieties resistant to Fox infection.
Observational study on the influence of an app-based self-management program on the quality of life of women with endometriosis.
Authors: Rohloff Nadine , Rothenhöfer Markus , Götz Teresa , Schäfer Sebastian 
Published: November 2022

Abstract
Purpose: Endometriosis can significantly impair the quality of life of those affected. Multimodal self-help measures are recommended but often difficult to access. Smartphone apps have been shown to improve quality of life for other conditions with chronic pain. The aim of this study was to examine whether there is evidence of beneficial effects of the smartphone app “Endo-App®” and whether a multicenter randomized controlled trial should be planned to substantiate these effects.
Methods: In a sample of N=106 women affected by endometriosis the present study determined the influence of the use of Endo-App ® on their quality of life. Among others, the validated questionnaire Endometriosis Health Profile from Oxford University was used for this purpose.
Results: The use of Endo-App ® lead to a highly significant improvement in quality of life already after two weeks. A statistically significant change was found for nine out of ten measured variables of quality of life. A series of further analyses validated that the measured positive effects were not due to other confounding factors.
Conclusion: In summary, the results indicate that the quality of life of women with endometriosis improved by the digital self-management tool Endo-App ®. More studies are needed to further explore the influence of the app on quality of life and as confirmatory evidence of beneficial effects. For this purpose, a randomized controlled trial should be conducted over a longer period of time.
Trial registration:
This trial is registered at clinicaltrials.gov under the registration number NCT05528601 on August 18, 2022. It was retrospectively registered.
Modification on nanocellulose extracted from kenaf (Hibiscus cannabinus) with 3-aminopropyltriethoxysilane for thermal stability in poly (vinyl alcohol) thin film composites
Authors: Taib Mohamad Nurul Azman Mohammad , Yee Tan Sue , Hussin M. Hazwan 
Published: November 2022

Abstract
Improvement in thermal stability of nanocellulose was done with silanization treatment, which started with the treatment of silylated kenaf nanocellulose (SKNC) using 3-aminopropyltriethoxysilane (APTES) in alcohol solution at 25°C with pH 4 within 4 hours. Then, SKNC with varied content from 0.5 to 2% (w/w) was reinforced in poly(vinyl alcohol) (PVA). The SKNC was characterized by FT-IR, XRD, TEM, and TGA. The peak at 1571 cm− 1 corresponding to N-H bending vibration was observed on SKNC spectra indicating a successful silane treatment follow-up by a reduction of peak 1642 and 1425 cm− 1, respectively. The crystallinity index for SKNC was increased to 70.34% as compared with KNC (61.73%). TEM showed a needle-like structure for both SKNC and KNC. The interaction between SKNC and PVA was characterized by FT-IR with an increment of peak 1089 and 1028 cm− 1, which shows the physical interaction that occurred between PVA and SKNC. The dispersion of SKNC was well homogenous as observed in FESEM. SKNC exhibited good thermal stability and a less amount of char was formed at 700°C compared to kenaf extractive free. Furthermore, TGA demonstrated improvement after addition of 2% SKNC into PVA as well as DSC increased the melting point of PVA.
SLC43A3 Promotes Oncogenesis and Predicts Poor Prognosis in Glioma
Authors: Li Jiaqi , Luan Xingzhao , Cen Yulin , Jiang Gen , Zhao Mingkuan , Xiang Wei , Zhou Jie 
Published: November 2022

Abstract
Purpose
Solute Carrier Family 43 Member 3 (SLC43A3) contributes to nucleobase transport in many metabolic diseases and is present as a fusion oncogene in some tumors. However, the potential clinical significance of SLC43A3 in the prognosis and progression of gliomas remains incompletely understood. The present study investigated the prognostic significance of SLC43A3 in glioma and the potential functional roles of SLC43A3 in this context.
Methods
A comprehensive analysis of two patient cohorts allowed us to establish a glioma prognosis model based on SLC43A3 expression. The biological processes related to SLC43A3 in glioma were investigated using functional enrichment analysis. Single-sample gene set enrichment analysis (ssGSEA) was used to evaluate the correlation between SLC43A3 and immune cell infiltration. Furthermore, SLC43A3 expression was detected in glioma patient samples, and functional analyses using siRNA knockdown were performed in U87-MG and U251 cell lines. Cell proliferation and apoptosis were measured using a cell counting kit-8 assay and flow cytometry. A transwell assay was used to assess cell migration.
Results
In glioma patients, high SLC43A3 expression negatively correlated with overall survival (OS) and progression-free survival (PFS), suggesting that the SLC43A3 expression level was an independent prognostic indicator that contributed to the pathogenesis of glioma, including tumor cell proliferation, migration, and apoptosis.
Conclusion
SLC43A3, an independent prognostic indicator, may be involved in the proliferation and migration of glioma cells and reduce the cell apoptosis to participate the carcinogenic pathway of glioma.