bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insulin-Like Growth Factor Binding Protein-3 Induces Senescence by Inhibiting Telomerase Activity in MCF-7 Breast Cancer Cells
Authors: Kwon Ahreum , Chae Hyun Wook , Lee Woo Jung , Kim JungHyun , Kim Ye Jin , Ahn Jungmin , Oh Youngman , Kim Ho-Seong 
Published: October 2022

Abstract
Insulin-like growth factor binding protein-3 (IGFBP-3) has been known to inhibit the proliferation of various cell types in an insulin-like growth factor (IGF)-independent manner. In this study, we aimed to show that IGFBP-3 induces cellular senescence via suppression of telomerase activity, thereby inhibiting cancer cell proliferation. We found that the induction of IGFBP-3 in MCF-7 cells inhibited cell proliferation. Flow cytometry revealed that the percentage of non-cycling cells was higher in IGFBP-3-expressing cells than in controls. Induction of IGFBP-3 also resulted in morphological changes, such as a flattened cytoplasm and increased granularity, suggesting that IGFBP-3 induces senescence-like phenotype. The percentage of cells containing senescence-associated β-galactosidase activity was 3.3 times higher in IGFBP-3 expressing cells compared to control cells. Telomeric repeat amplification and real-time PCR showed that IGFBP-3 decreased telomerase activity by decreasing the expression of the RNA component (hTR) and catalytic protein component with reverse transcriptase activity (hTERT) of telomerase. These results suggest that IGFBP-3 functions as a negative regulator of breast cancer cell growth by inducing a senescence through the inhibition of telomerase activity.
Study on Deep drawing Forming Process of Wheel Disks
Authors: Song Jie , Feng Jun , Li Junzhou , Hou Rui , Tian Haiping , Sun An 
Published: October 2022

Abstract
In the deep drawing forming process of wheel disks, the wall thickness at the rounded corners tends to be thinned. Using DEFORM software analysis, two stages of the deep drawing forming process are used to study the wheel disk forming. A finite element model of deep drawing and forming was established based on the theoretical analysis of the deep drawing and forming process. The stress and strain field distribution during the forming process of the circular blank was simulated and analyzed. The analysis shows that different punch feed rates and friction coefficients greatly influence the thinning rate and forming load of the wheel forming. The thinning rate reached 15.4% when the punch feed rate was 180 mm/s. The maximum forming load increases with the increase of friction coefficient, and the total forming load reaches 424 kN when the friction coefficient is 0.20. The effect of r p /t 0 and r d /t 0 on the wall thickness of the second pass forming was also analyzed. The results show that when r p /t 0 is 1.6~2.4 and rd/t0 is 2~3, the minimum wall thickness is larger, ΔX is also the smallest, and the forming quality is the best. The optimal set of parameters was obtained: punch feed rate of 120 mm/s, friction coefficient of 0.12, and r p /t 0 of 2.4. Physical tests were conducted on the self-designed forming machine, and the results were consistent with the simulation results, which verified the feasibility of the forming solution.
Risk factors for nonhealing wounds and the clinical value of the gracilis-myofascial flap in abdomino-perineal resection: a case control study
Authors: Lin Zhiyu , Zhang Xinling , Sun Yidan , Chen Yujie , Yang Xin , Zhao Zhenmin 
Published: October 2022

AbstractBackground:Risk factors for nonhealing wounds after abdomino-perineal resection (APR) remain inconclusive. And the pelvic floor reconstruction after APR based on gracilis-myofascial flaps (GMFs) is still controversial. This study aims to evaluate the risk factors for nonhealing wounds and demonstrate the clinical application of GMF reconstruction after APR.Methods:APR patients at Peking University Third Hospital from December 2010 to January 2021 were analyzed retrospectively, including 81 with primary suture and 5 with GMF reconstruction. The data collected included general information, comorbidities, pre/postoperative laboratory tests, surgical data and pathological results. Univariate analysis and multifactor binary logistic regression analysis was used.Results:There were 62 (71.2%) patients in the healing group (HG) and 24 (27.9%) patients in the nonhealing group (nHG). Old age (p = 0.011), hyperlipidemia (p = 0.042) and higher pathological Broders Grading (p = 0.048) were risk factors for nonhealing wounds. The mean hospitalization time (p = 0.002) and expense (p = 0.017) of the nHG were greater than HG. Multifactor binary logistic regression analysis showed age and hyperlipidemia were independent risk factors for nonhealing wounds after APR. Five patients with GMF reconstruction recovered well without any postoperative complications.Conclusion:Old age, hyperlipidemia and high Broders Grading are risk factors for nonhealing perineal wounds after APR, and age and hyperlipidemia are independent risk factors. GMF has satisfactory outcomes for pelvic floor reconstruction and lower probability of nonhealing wounds. Our study may provide new insights for the prevention and treatment of nonhealing perineal wounds after APR.
Adsorption of Arsenic From Synthetic Wastewaters: Linear and Non-linear of 2-parameters Adsorption Equilibrium Isotherm Models
Authors: AMOKO Justinah Solayide , OJO Babatunde Moses , BABATOLU Ayotunde Oluwaseun , DEMEHIN Abidemi Iyewumi , OKE Isaiah Adesola 
Published: October 2022

Abstract

Contamination of groundwater with arsenic (Asic) has become emerging health and environmental problem around the world. This problem has received signiﬁcant attention amongst scientists for the development of new adsorbents to remediate Asic -contaminated water. The ability of the immobilized powdered eggshell (Poes), as adsorbent, to remove Asic was studied under batch conditions. Equilibrium data were analysed using non-linear and linearized two-parameter adsorption isotherms models (Langmuir, Freundlich, Elovich, Flory–Huggins, Temkin, Frenkel- Hasley- Hill; Langmuir- Vageler, Hill-de Boer, Kiselev, Fowler- Guggenheim, Dubinin – Radushkevich, Jovanovic, Harkins–Jura and Halsey). The performance of adsorption equilibrium isotherm models was evaluated statistically using the following analysis of variance (ANOVA), model of' selection criterion (MSC), Coefficient of Determination (CD), Correlation coefficient (R) and Akaike Information Criterion (AIC). The study revealed that for non-linear equilibrium isotherm models, Freundlich (0.986 and 3.906) > Fowler—Guggenheim (0.996 and 5.176) and Hasley (0.986 and 3.906) performed well in predicting experimental data based on the values of R and MSC. For linearized equilibrium isotherm models, Kiselev (0.9999 and 8.856) > Temkin (0.994 and 4.701) and Dubinin – Radushkevich (0.993 and 4.621) are the best isotherm models for Asic adsorption onto Poes. It was concluded that Poes particles are with various inorganic materials for Asic adsorption. Based on the performance indicators and to ensure reliable results of adsorption equilibrium data analysis through the adsorption isotherm models, it is necessary that these data sets should be evaluated by both linear and non-linear regression analyses.
The application research of deep neural networks in colonic polyp segmentation
Authors: LI Jun , Wang Fang , Yang Haima , Qiu Lihua , Liu Jin , Fu Le , Zhang Dawei , Hong Weibin , Song Yeye 
Published: October 2022

Abstract
Background: In the study of medical images segmentation, U-Net is a good choice with better performance. But it will cause some problems such as gradient problems or information loss.
Methods: In this paper, we introduce a new model based on such an encoder-decoder structure. We mixed a full connection and encoder output in per layer and we add an axial attention to solve gradient problem and keep the connection with pixel from long distance. After pooling, we add a module which has three atrous Convolution processes with different expansion rate and a non-local Self attention to increase the feeling field and fix the problem of losing spatial information. In decoder part, we put a dual channel gate to point out 'what' and 'where' feature we need by the union of Channel Attention and Spatial Attention.
Results: We use 4 public dataSets are CVC-ClinicDB, Kvasir-SEG, CVC-ColonDB and CVC-T. We compared more than 10 groups other different models with these 4 group dataSets and marked some evaluating indicators such as mIou, mDice and so on. We also did some ablation experiments to verify that our model structure is reasonable and effective.
Conclusions: The comparative training of 12 models in 4 different datasets verifies the feasibility and the good effectiveness of the proposed ideas. We also did some ablation experiments to verify that our model structure is reasonable and effective. The basic structure of the encoder decoder in semantic segmentation has been greatly improved.
WITHDRAWN: Low-intensity pulsed ultrasound attenuates postoperative neurocognitive impairment and salvages hippocampal synaptogenesis in aged mice
Authors: Wang Qian , Liu Taotao , Chang Huixian , Li Zhengqian , Chen Lei , Xing Huayi , Wang Xiaoxiao , Hong Jingshu , Liu Kaixi , Li Yitong , Han Dengyang , Li Yue , Yang Ning , Li Xiaoli , Li Yingwei , Guo Xiangyang 
Published: October 2022

Abstract

Background Postoperative neurocognitive impairment is an urgent problem with global aging accelerating. The prevention and treatment of postoperative neurocognitive impairment have been widely investigated but lack effective strategies. Low-intensity pulsed ultrasound (LIPUS), a non-invasive tool, has shown an effect on neuroprotection, but whether it could attenuate the postoperative neurocognitive impairment and the underlying mechanism remains unknow. Methods An experimental set-up for LIPUS stimulation of the hippocampus was well established. HE staining was used to determine the morphology of hippocampus after LIPUS application. A laparotomy model in aged mice was applied, and Morris water maze was used to assess cognitive function. RT-qPCR and western blotting were used to detect levels of inflammatory cytokines, synapse associated protein in the hippocampus, respectively. Immunofluorescent staining was also used to determine the neural activation and Piezo1 expression. Results After the anesthesia/surgery, the hippocampus of aged mice showed increased levels of inflammation and decreased synaptogenesis, while LIPUS intervention improved cognitive impairment, reduced hippocampal neuroinflammation, and improved synaptogenesis. We further found that LIPUS might improve synaptogenesis through the Piezo1-mediated calpain1/extracellular regulated protein kinases (Erk) pathway. Conclusion Our results suggested that LIPUS could be used as an effective physical intervention to improve postoperative cognitive dysfunction in the aged population.
Measurement and evaluation of noise levels inside and outside the industrial area of Ferronikeli
Authors: Veliu Agron 
Published: October 2022

Abstract
The increasing awareness of the public of environmental issues such as CO2 emissions, carbon footprints and waste recycling has brought the issue of environmental noise to the fore.
Many of the demands of modern society result in the creation of noise sources such as larger airports, additional power stations or heavy industry and higher road traffic levels. In addition, the expansion of towns and cities, driven by the demand for housing, has often resulted in the development of land near existing noise sources. These trends have meant that many more industries are now required to monitor their environmental noise impact.
Environmental noise, caused by traffic, industrial and recreational activities, is one of the main local environmental problems in many countries of Europe and the source of an increasing number of complaints from the public.
Generally, however, action to reduce environmental noise has had a lower priority than that taken to address other environmental problems such as air and water pollution.
In the past, the noise generated by different sources, including industries, was not as obvious in trends within Kosovo Country. Gllogoc is a town that is a part of the Pristina region, located in the central part of the Republic of Kosovo. In this town the smelter plant ‘NewCo Ferronikeli’ which is well known as an industrial complex for ferronickel alloy production is located.
This study was a simple assessment of the noise levels around the NewCo Ferronikeli company. However, this study is based on the Directive 2002/49/EC of The European Parliament and of the Council of 25 June 2002 relating to the assessment and management of environmental noise.
Dietary Patterns and the Risk of Sarcopenia: a cross‑sectional study of 1059 participants in China
Authors: Wang Boshi , Zhang Xue , Shao Lin , Li Wei , Zhao Shilong , Liu Peng 
Published: October 2022

Abstract
Background: Sarcopenia, defined as the loss of muscle mass and strength, has been associated with increased hospitalization and mortality. Due to different diagnostic criteria and target populations, the prevalence of sarcopenia varies greatly. Dietary pattern analysis is a whole diet approach used to investigate the relationship between diet and sarcopenia. This study aims to estimate the prevalence of sarcopenia and explore possible factors associated with it among a large population in Beijing, China.
Methods: A cross-sectional study with 1059 participants aged more than 50 years was performed. Sarcopenia was defined based on the guidelines of the Asian Working Group for Sarcopenia. The total score of the MNA-SF questionnaire was used to analyse nutrition status. The baseline demographic information, diet structure and eating habits were collected by clinicians trained in questionnaire data collection and anthropometric and bioimpedance measurements.
Results: The overall prevalence of sarcopenia was 8.8% and increased with age: 5%, 5.8%, 10.3% and 26.2% in the 50-59, 60-69, 70-79, and ≥80 years groups, respectively. Marital status (with or withouta spouse) was not an independent factor associated with sarcopenia orincome status. However, nutritional risk or malnutrition, vegetable diet, advanced age and spicy eating habits were risk factors for sarcopenia. Meanwhile, daily fruit, dairy and nut consumption were protective factors against sarcopenia.
Conclusions: Although further studies are required to explorethe association between healthy dietary patterns and the risk of sarcopenia, the present study would help to provide basic data for establishing dietary guidelines for the prevention of sarcopenia in Chinese elderly individuals.
M0 pancreatic cancer: treatment and prognosis by primary site
Authors: Deng Zhongming , Zhang Zelin , Ding Zhenghua , Cao Ping 
Published: October 2022

Abstract
Background
Pancreatic cancer is very individual and treatment varies from patient to patient. The aim of this study was to evaluate the effects of surgery, radiotherapy and treatment patterns on the risk of death in patients with non-metastatic pancreatic cancer with different primary sites. There have been few studies on the incidence and survival of patients with non-metastatic pancreatic cancer. Our aim was to examine the difference in prognosis between head and body and tail tumors in patients with non-metastatic pancreatic cance. And, the difference of radiotherapy and chemotherapy in different primary sites.
Methods
A retrospective cohort study of patients with non-metastatic pancreatic cancer between 2004 and 2016 obtained from the SEER database. SPSS software was used for analysis. We use kaplan-Meier method based on logarithmic rank test to estimate survival distribution. COX univariate and multivariate regression analyses were performed with variables such as race, sex, age at diagnosis, stage, surgery, and radiotherapy. A propensity score matching model was used to further validate prognostic factors associated with survival.
Results
For 5859 M0 pancreatic cancer patients in the SEER database, ancreatic head tumors accounted for 4824 (82.3%) and pancreatic tail tumors accounted for 1034 (17.7%). The incidence of LN + was higher in pancreatic head than in pancreatic bodytail tumors (OR 1.621, 95%CI 1.381–1.917, P < 0.01). 91.0% of pancreatic head tumor patients and 89.2% with pancreatic bodytail tumor patients received chemotherapy. More pancreatic head tumors were treated with radiation (73.3% vs 66%, P < 0.05) than pancreatic bodytail tumros. for pancreatic head tumors, size, Grade amd T stage, LN+, chemotherapy, and radiotherapy are risk factors of survival。However, size, LN+, and chemotherapy showed no association to the prognosis of pancreatic bodytail tumors. Primary site is not an independent risk factor affecting prognosis. We further verified the above conclusion with a 1:1 propensity score matching.
Conclusion
We found that primary site is not a risk factor for survival of pancreatic cancer patients, but pancreatic head and body tail pancreatic cancer have different characteristics. In addition, the effects of radiation and chemotherapy differed in the two groups.
Human and infrastructure exposure to large wildfires in the United States
Authors: ModaresiRad Arash , Abatzoglou John , Kreitler Jason , Alizadeh Mohammad Reza , AghaKouchak Amir , Hudyma Nicholas , Nauslar Nicholas , Sadegh Mojtaba 
Published: October 2022

Abstract
An increasing number of wildfire disasters occurred in recent years in the U.S. Here, we demonstrate that cumulative primary human exposure – population residing within large wildfires’ perimeters – was 594,850 people from 2000-2019 across the Contiguous U.S. (CONUS), 82% of which occurred in the Western U.S. Primary population exposure increased by 125% in CONUS in the past two decades, noting large uncertainty ranges. We show that population dynamics from 2000-2019 alone accounted for 24% of the observed increase rate in human exposure, whereas increased wildfire extent drove a majority of the observed trends. Additionally, we document widespread exposure of roads (412,155 km) and transmission powerlines (14,835 km) to large wildfires in CONUS, with an increased rate of 58% and 70% from 2000-2019, respectively. Our findings highlight the benefits of mitigation and adaptation efforts to help societies cope with wildfires.
Highly coherent nitrogen-vacancy centres in diamond via rational high-pressure and high-temperature synthesis and treatment
Authors: Zhang Xiaoran , Liu Kang-yuan , Li Fengjiao , Liu Xiaobing , Duan Shuai , Wang Jia-Ning , Liu Gang-Qin , Pan Xin-Yu , Chen Xin , Zhang Ping , Ma Yanming , Chen Changfeng 
Published: October 2022

Abstract
Nitrogen-vacancy (NV-) centres in diamond offer an excellent platform for device design and development in the emerging fields of quantum sensing and quantum network, but fabrication of quantum-grade diamonds with highly coherent NV- centres needed for advanced applications presents a pressing challenge. Here we report a major advance in generating NV- centres in diamonds via tailored high-pressure and high-temperature (HPHT) synthesis and post-grown annealing treatment. The resulting well dispersed single NV- centres in type-IIa diamonds exhibit long spin coherence times reaching the highest levels achieved in diamonds prepared by more elaborate and restrictive chemical vapor deposition techniques. Moreover, we produce high-density NV- ensembles in <100>-grown type-Ib diamonds with superb zero-phonon lines considerably sharper than those of native NV- centres in as-grown diamonds. These findings demonstrate a superior synthesis and optimization protocol for creating top-quality NV- centres to meet the most stringent requirements for a wide range of emerging quantum technologies.
A screen for Plasmodium falciparum sporozoite surface protein binding to human hepatocyte surface receptors identifies novel host-pathogen interactions
Authors: Segireddy Rameswara R , Belda Hugo , Yang Annie S. P. , Dundas Kirsten , Knoeckel Julia , Galaway Francis , Wood Laura , Quinkert Doris , Knuepfer Ellen , Treeck Moritz , Wright Gavin J , Douglas Alexander D 
Published: October 2022

Abstract
Sporozoite invasion of hepatocytes is an essential step in the Plasmodium life-cycle and has similarities, at the cellular level, to merozoite invasion of erythrocytes. In the case of the Plasmodium blood-stage, efforts to identify host-pathogen protein-protein interactions have yielded important insights including vaccine candidates. In the case of sporozoite-hepatocyte invasion, the host-pathogen protein-protein interactions involved are poorly understood.
Here, we performed a systematic screen to identify such interactions. We substantially extended previous Plasmodium falciparum and human surface protein ectodomain libraries, creating new libraries containing 88 P. falciparum sporozoite protein coding sequences and 182 sequences encoding human hepatocyte surface proteins. Having expressed recombinant proteins from these sequences, we used a plate-based assay capable of detecting low affinity interactions between recombinant proteins, modified for enhanced throughput, to screen the proteins for interactions. We were able to test 7540 sporozoite-hepatocyte protein pairs under conditions capable of detecting interactions of at least 1.2µM KD.
We report and characterise an interaction between human fibroblast growth factor receptor 4 (FGFR4) and the P. falciparum protein Pf34, characterising its affinity and demonstrating blockade of the interaction by reagents including a monoclonal antibody. Furthermore, we identify further interactions between Pf34 and a second P. falciparum rhoptry neck protein, PfRON6, and between human low-density lipoprotein receptor (LDLR) and the P. falciparum protein PIESP15.
Conditional genetic deletion confirmed the essentiality of PfRON6 in the blood-stage, consistent with the important role of this protein in parasite lifecycle. Pf34 was refractory to attempted genetic modification. Antibodies to Pf34 abrogated the interaction and had a modest effect upon sporozoite invasion into primary human hepatocytes.
Pf34 and PfRON6 may be members of a functionally important invasion complex which could be a target for future interventions. The modified interaction screening assay, protein expression libraries and P. falciparum mutant parasites we report here may be a useful tool for protein interaction discovery and antigen candidate screening which could be of wider value to the scientific community.