bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between visit‐to‐visit blood pressure variability and adverse events in coronary artery disease patients after coronary intervention
Authors: Tsai Tsung‐Ying , Leu Hsin‐Bang , Hsu Pai‐Feng , Yang Ya‐Ling , Chen Su‐Chan , Huang Shao‐Sung , Chan Wan Leong , Lin Shing‐Jong , Chen Jaw‐Wen , Pan Ju‐Pin , Charng Min‐Ji , Chen Ying‐Hwa , Wu Tao‐Cheng , Lu Tse‐Min , Huang Po‐Hsun , Cheng Hao‐Min , Huang Chin‐Chou , Sung Shih‐Hsien , Lin Yenn‐Jiang , Wu Cheng‐Hsueh 
Published: September 2022

AbstractBlood pressure variability (BPV) is independently associated with higher cardiovascular risks. However, whether BPV is associated with poor outcomes for coronary artery disease (CAD) patients after percutaneous coronary intervention (PCI) remained undetermined. We aimed to investigate the relationship between BPV and the outcomes of CAD patients undergoing PCI. Two thousand seven hundred and sixty‐two CAD patients (1938 males, mean age 69.6 ± 12.9) who received PCI at Taipei Veterans General Hospital from 2006 to 2015 with multiple blood pressure measurements before and after the index PCI were enrolled. We calculated the standard deviation of systolic blood pressure, diastolic blood pressure, and pulse pressure as parameters of BPV. The primary endpoint was the composite of major adverse cardiovascular events [MACE comprising of cardiovascular death, nonfatal myocardial infarction (MI), and non‐fatal stroke] and heart failure hospitalization (HHF). The key secondary endpoint was MACE. Both pre‐PCI and post‐PCI BPV were associated with CV events even after adjusting for co‐morbidities and mean blood pressure. In Cox analysis, for every 1 mmHg increase in systolic BPV, the hazard ratio for the MACE + HHF, MACE, HHF, and cardiovascular death was 1.04 (95%CI: 1.03–1.05), 1.04 (95%CI: 1.02–1.05), 1.05 (95%CI: 1.04–1.06), and 1.06 (95%CI: 1.03–1.09), respectively. The association between BPV and cardiovascular risk is independent of blood pressure control status. The prognostic value of BPV was superior to mean blood pressure in both pre‐PCI and post‐PCI period. BPV is independently associated with cardiovascular events after PCI and has a better prognostic value than mean blood pressure suggesting the importance of maintaining stable blood pressure for CAD patients.
Home oral hygiene is associated with blood pressure profiles: Results of a nationwide survey in Italian pharmacies
Authors: Del Pinto Rita , Pietropaoli Davide , Grassi Guido , Muiesan Maria Lorenza , Monaco Annalisa , Cossolo Marco , Procaccini Alfredo , Ferri Claudio 
Published: September 2022

AbstractAimPeriodontal diseases are associated with cardiovascular risk factors/diseases, and whether home oral hygiene practices are inversely related to the same conditions could carry relevant practical implications. We investigated the association of home oral hygiene habits with hypertension.Materials and MethodsDuring World Hypertension Day 2020, a nationwide cross‐sectional survey was conducted on volunteers ≥18 years at 733 Italian pharmacies. Participants underwent standardized blood pressure (BP) measurement and answered a questionnaire on cardiovascular risk factors, oral health status, and home oral hygiene habits (toothbrushing daily frequency and manual/electric toothbrush). The association between home oral care habits and BP was assessed using multivariate logistic regression. Interactions between exposures and outcome were formally tested.ResultsAmong the 4506 participants (44.8% males, 66.1 ± 37.8 years), 47.6% reported brushing ≥3 times/day and 23.4% declared using the electric toothbrush. Brushing ≥3 versus <3 times/day and use of electric versus manual toothbrush were associated with 19% (odds ratio [OR]: 0.81, 95% confidence interval [CI] 0.70–0.94) and 28% (OR: 0.72, 95% CI 0.61–0.85) lower odds of hypertension, respectively. No significant additive interaction was observed in the association of exposures with the outcome.ConclusionsRegular daily brushing and electric toothbrushing are associated with a better BP profile in a real‐world context. Future interdisciplinary research is warranted to test these findings.
Influence of far‐infrared vacuum drying on drying kinetics and quality characteristics of Cistanche slices
Authors: Jiang Chunhui , Wan Fangxin , Zang Zepeng , Zhang Qian , Xu Yanrui , Huang Xiaopeng 
Published: September 2022

AbstractIn order to improve the drying efficiency and quality of Cistanche, the effects of far‐infrared vacuum drying on drying rate, active component content and microstructure of Cistanche slices were studied with drying temperature, slice thickness and irradiation height as experimental indexes. The results showed that the drying process of Cistanche was mainly concentrated in the deceleration stage. When the drying temperature was 55°C, the slice thickness was 4 mm, the irradiation height was 150 mm, and the time to reach the safe water content (10% w.b.) of Cistanche was the shortest. With the increase in drying temperature, slice thickness and irradiation height, the contents of phenylethanoid glycosides, iridoids, polysaccharides, total phenols and total flavonoids in dried products increased first and then decreased. Compared with natural drying, the samples dried by far‐infrared vacuum have more pores and better structural integrity. Phenylethanol glycosides and iridoid compounds, such as echinacoside and verbascoside, in Cistanche have significant effects in anti‐inflammatory and analgesic, tonifying kidney yang, anti‐Alzheimer's disease and protecting various organs and systems of the body. In this study, the slices of Cistanche were processed by far infrared vacuum drying, and the effects of different drying conditions on drying rate, effective moisture diffusion coefficient, active ingredient content, antioxidant activity, color and microstructure of dried Cistanche were studied. The drying process was fitted by a drying model, in order to obtain dried Cistanche products with better quality, and provide theoretical basis and technical support for the actual production of Cistanche.Novelty impact statement

Midilli model could better describe the variation law of water content in the drying process of Cistanche.

Compared with natural drying, the surface samples dried by far‐infrared vacuum have no cracks and good boundary integrity.

The optimal process parameters (55°C, 4 mm, 310 mm) for far‐infrared vacuum drying of Cistanche slices were determined by the entropy value method.
Efficacy of Withania coagulans fruit extract as a coagulant for Mozzarella cheese at different coagulation temperatures from curd formation to pizza top
Authors: Nazish Hafiz Adeel , Gulzar Nabila , Nadeem Muhammad , Rafiq Saima , Sameen Aysha , Ajmal Muhammad , Murtaza Shamas , Saleem Iqra Muqaddas 
Published: September 2022

AbstractWithania coagulans is a shrub that has milk‐clotting activity. Its coagulation efficiency at different concentrations and incubation temperatures was measured for Mozzarella cheese from curd formation to its application on the pizza top. Curd made at higher W. coagulans concentration (1%) has less coagulation time (1272.99 ± 96.68 s), viscosity (148.58 ± 9.25 Pa s), pH (5.89 ± 0.05), and yield (7.87 ± 0.53%) as compared to rest of samples. Increasing temperature (39°C) also decreased coagulation time (1599.19 ± 380.06 s), viscosity (146.56 ± 15.51 Pa s), pH (6.01 ± 0.17), and yield (8.87 ± 0.48%) of curd. W. coagulans at 0.2% concentration showed caseinolytic activity (78.83 ± 1.97%) very close to rennet (84.12 ± 2.3%). High W. coagulans and temperature conditions lead to the grainy texture that results in the loss of proteins, fats, and moisture from the cheese. Highest protein (21.89 ± 0.8%; 23.39 ± 2.15%), fat (17.64 ± 0.62%; 18.50 ± 0.32%), and moisture (43.55 ± 8.01%; 50.00 ± 6.13%) were observed in Mozzarella cheese at 0.2% W. coagulans concentration and 33°C temperature, respectively. This study showed that 0.2% extract of W. coagulans can be used to clot the milk for cheese production at 33°C.Novelty impact statementThe interest in plant‐based coagulant consumption has been growing because of the rennet shortage and religious matters of some vegetarian people. A plant‐based coagulant from Withania coagulans has been reported for its milk‐clotting activity, but its behavior for Mozzarella cheese production from curd formation to its application on pizza topping needs detailed study for its use on an industrial scale. In this study, W. coagulans behavior was studied during curd formation. Its caseinolytic and proteolytic activities in comparison to rennet were studied. Mozzarella cheese physicochemical and functional characteristics on pizza topping were also described in this research.
Effectiveness of preventive B‐Lynch sutures in patients at a high risk of postpartum hemorrhage
Authors: Kuwabara Mayuka , Takahashi Yuichiro , Iwagaki Shigenori , Imai Noriaki , Asai Kazuhiko , Matsui Masako , Shimaoka Ryuichi , Ono Hitomi 
Published: September 2022

AbstractAimWe aimed to evaluate the clinical outcomes and adverse events of preventive B‐Lynch suture performed during cesarean section in patients at a high risk of postpartum hemorrhage.MethodsThis retrospective observational study included patients who underwent a cesarean section and the B‐Lynch suture at a tertiary perinatal medical center between January 2019 and May 2021. The B‐Lynch sutures were placed preventively before excessive blood loss occurred in patients with uterine atony, placental position abnormality (placenta previa and low‐lying placenta), placenta accreta, thrombocytopenia, coagulopathy, and other risk factors of bleeding. Partial compression sutures for bleeding points and vaginal gauze packing were placed if required.ResultsThe B‐Lynch suture was performed in 38 patients, and hysterectomy was avoided in all patients. Only one patient required intrauterine balloon tamponade as an additional treatment 5 days after the cesarean section. No apparent postoperative bleeding occurred within 2 h after the cesarean section in 35 patients (92%), and blood transfusion was avoided in 14 patients (37%). Thirty‐three adverse events occurred in 23 patients; these included an inflammatory response, hematomas, retained products of conception, and ileus in one, two, and two patients, respectively. In most cases, the events were not severe and were unrelated to the procedure. In one patient, a second‐look operation was performed and no complications were observed in the uterus and abdominal cavity.ConclusionsPreventive B‐Lynch suture seemed effective and safe after a short‐term observation. When excessive bleeding is expected during a cesarean section, an early introduction of this procedure is recommended.
Effect of in ovo administration of Newcastle disease vaccine conjugated with Astragalus polysaccharide on growth performance, intestinal development, and mucosal immunity in broiler chickens
Authors: Xue Ligang , Zhang Yingnan , Wang Dan , Luan Weimin , Yang Shubao 
Published: September 2022

AbstractThis study was conducted to investigate the effect of in ovo administration of a mixture of Astragalus polysaccharide (APS) and Newcastle disease vaccine (NDV) on growth performance, intestinal development, and mucosal immunity in newly hatched chicks. Six hundred specific‐pathogen‐free (SPF) Leghorn fertilised eggs were incubated in a commercial hatchery and divided into four groups: (a) control group injected with 1 ml of 0.9% physiological saline, (b) APS group injected with 1 ml of 1 mg/ml APS solution, and (c) NDV group injected with 1 ml of 104.0 EID50/dose of NDV solution, and (d) APS + NDV group injected with a mixture of 0.5 ml of 2 mg/ml APS plus 0.5 ml 104.0 EID50/dose ND vaccine (NDV) on Day 18.5 of incubation. The results showed that in ovo injection of APS or the mixture of APS and NDV increased the body weight at 1 day (IW) and final weight (FW) at 28 days and increased the feed conversion ratio (FCR) at 1–7, 8–14, 15–21, and 1–28 days of age. The villus height (VH) was increased (p < 0.05), and the crypt depth (CD) was decreased (p < 0.05) in the duodenum compared with the control group. The VH/CD ratios were increased (p < 0.05) in the APS + NDV group compared with controls, NDV group, and APS group on d3. The levels of slgA in washings were increased (p < 0.05) on Days 3, 7, 14, 21, and 28, and the number of IgA+ cells in the duodenum was increased on Days 7, 14, 21, and 28. In addition, the IgA+ cells were promoted from the villus root to the apex in the APS + NDV group. It can be concluded that in ovo administration of NDV conjugated with APS compared with NDV alone may be more effective in promoting growth performance and intestinal mucosal immunity.
Short‐ and long‐term outcomes of polyethylene band attenuation of congenital extrahepatic portosystemic shunts in dogs: 60 cases (2010‐2020)
Authors: Glenn O. , Tomlinson A. , Pinchbeck G. , Burrow R. 
Published: September 2022

ObjectivesTo report the short‐ and long‐term outcomes following attenuation of congenital extrahepatic portosystemic shunts in dogs using a novel polyethylene band.Materials and MethodsRecords were retrospectively reviewed for dogs that underwent congenital extrahepatic portosystemic shunt attenuation by a polyethylene banding technique, at a single institution between 2010 and 2020. Short‐term outcome data were collected from peri‐operative clinical records with follow‐up examinations, scheduled at 6 and 18 weeks post‐operatively, and post‐operative imaging when performed. Long‐term follow‐up was collected by validated owner questionnaire, telephone interview or medical records. Long‐term outcomes were categorised by “excellent”, “good” or “poor”.ResultsSixty dogs were included. Post‐operative complications occurred in 10 of 60 dogs (16.7%), four major and six minor, with a peri‐operative mortality of 6.7%. Persistent shunting was identified in nine of 53 dogs (17%) available for follow‐up examination and four dogs underwent a revision surgery. Long‐term follow‐up was available for 44 dogs at a median of 75 months post‐operatively (range 7 to 128). Long‐term outcomes were “excellent” (26) or “good” (8) in 81.8% of dogs and “poor” (8) in 18.2%. At the time of follow‐up, 30 of 44 (68.2%) dogs were not receiving any medical treatment and 27 of 28 (96.4%) questionnaire respondents were satisfied with the response to surgery.Clinical SignificancePolyethylene band attenuation of congenital extrahepatic portosystemic shunts provides comparable outcomes to cellophane. The material used in this study is widely available and consistent while being pre‐sterilised and pre‐folded makes it easy to use.
Neurological disease suspected to be caused by tick‐borne encephalitis virus infection in 6 horses in Switzerland
Authors: Magouras Ioannis , Schoster Angelika , Fouché Nathalie , Gerber Vinzenz , Groschup Martin H. , Ziegler Ute , Fricker Raffael , Griot Christian , Vögtlin Andrea 
Published: September 2022

AbstractBackgroundReports on acute tick‐borne encephalitis virus (TBEV) infections with signs of neurologic disease in horses are limited.ObjectivesTo describe the epidemiological, clinical, and laboratory findings of suspected acute TBEV infections in 6 horses.AnimalsSix horses originating from TBEV endemic regions of Switzerland were presented to equine hospitals with acute onset of neurologic disease between 2011 and 2019.MethodsRetrospective case series. Horses with acute onset of signs of neurologic disease that were subjected to clinical and microbiological examinations to rule out infectious diseases affecting the central nervous system.ResultsAll horses exhibited acute signs of neurologic disease including ataxia and proprioceptive deficits. Horses tested positive for TBEV using virus neutralization test and samples were further tested for TBEV‐specific IgM. The presence of TBEV‐specific IgM antibodies was confirmed in 5 horses (cases 1‐5, Laboratory Unit [LU] values ranging from 30 to 56). One horse (case no. 6) with an LU value just below the test threshold (LU = 22.3) was also included under the hypothesis that the horse was transitioning from acute to chronic infection. All horses originated from areas where humans with confirmed tick‐borne encephalitis reported to have been bitten by ticks.Conclusions and Clinical ImportanceAcute TBEV infection should be a differential diagnosis in horses with signs of neurologic disease and originating from TBEV endemic areas. The establishment of harmonized diagnostic criteria would help to overcome the diagnostic challenges associated with TBEV and other Flavivirus infections in horses.
Metabolism and residue differences of Enrofloxacin between the brain and peripheral tissues and the resulting brain damages in crucian carp (Carassius auratus var. Pengze)
Authors: Wan Gen , Zhang Fan , Wang Runping , Wei Lili , Huang Jianzhen , Lu Xinmin , Cai Zhihuan , Wang Long , Zhong Zhiwei , Xu Yanyan , Ruan Jiming 
Published: September 2022

AbstractThis study aimed to explore the metabolism and residue differences of Enrofloxacin (ENR) at two doses between the brain and peripheral tissues (liver, kidney, and muscle) along with the brain damages caused by ENR in crucian carp (Carassius auratus var. Pengze). The concentrations of ENR in tissues were determined using a validated high‐performance liquid chromatography (HPLC) analysis. Relying on the hematoxylin–eosin (HE) staining method, brain damages caused by the drug were evaluated by the section of pathological tissue. Metabolism and residue results showed that ENR could be detected in the brain throughout the experiment both at median lethal dose (LD50 at 96 h, 1949.84 mg/kg) and safe dose (SD, 194.98 mg/kg), as well as in the three peripheral tissues. The maximum residue at LD50 followed the decreasing order of liver >kidney > brain > muscle. Although the Cmax of ENR at SD in the brain was significantly lower than that in other peripheral tissues (p < .05), it still reached 41.91 μg/g. The T1/2 of ENR in brain tissue at the same dose was both shorter than that in peripheral tissues. At LD50, the amount of ENR residues in brain was lower than that in peripheral tissues on the whole, except that it had been higher than in the muscle for the first 3 h. At SD, the drug residue in brain tissue was lower than that in peripheral tissues from 12 h to 960 h, but it exceeded the muscle and kidney at 1 h and 6 h, respectively. At 960 h, the residual amount of ENR at SD in the brain was 0.09 μg/g, while it was up to 0.15 μg/g following the oral administration at LD50. Demonstrated by the HE staining, there were pathological lesions caused by ENR in the brain at LD50, which were characterized by sparse neural network and increased staining of glial cells. The present results indicated that metabolism and residue of ENR in crucian carp were affected by the tissue type and drug dosage, and the ENR could also bring about histopathological changes in the brain.
Two is better than one: Coupling DNA metabarcoding and stable isotope analysis improves dietary characterizations for a riparian‐obligate, migratory songbird
Authors: Hoenig Brandon D. , Trevelline Brian K. , Kautz Andrea , Latta Steven C. , Porter Brady A. 
Published: September 2022

AbstractWhile an increasing number of studies are adopting molecular and chemical methods for dietary characterization, these studies often employ only one of these laboratory‐based techniques; this approach may yield an incomplete, or even biased, understanding of diet due to each method's inherent limitations. To explore the utility of coupling molecular and chemical techniques for dietary characterizations, we applied DNA metabarcoding alongside stable isotope analysis to characterize the dietary niche of breeding Louisiana waterthrush (Parkesia motacilla), a migratory songbird hypothesized to preferentially provision its offspring with pollution‐intolerant, aquatic arthropod prey. While DNA metabarcoding was unable to determine if waterthrush provision aquatic and terrestrial prey in different abundances, we found that specific aquatic taxa were more likely to be detected in successive seasons than their terrestrial counterparts, thus supporting the aquatic specialization hypothesis. Our isotopic analysis added greater context to this hypothesis by concluding that breeding waterthrush provisioned Ephemeroptera and Plecoptera, two pollution‐intolerant, aquatic orders, in higher quantities than other prey groups, and expanded their functional trophic niche when such prey were not abundantly provisioned. Finally, we found that the dietary characterizations from each approach were often uncorrelated, indicating that the results gleaned from a diet study can be particularly sensitive to the applied methodologies. Our findings contribute to a growing body of work indicating the importance of high‐quality, aquatic habitats for both consumers and their pollution‐intolerant prey, while also demonstrating how the application of multiple, laboratory‐based techniques can provide insights not offered by either technique alone.
The genome of Areca catechu provides insights into sex determination of monoecious plants
Authors: Zhou Guangzhen , Yin Hongyan , Chen Fei , Wang Yicheng , Gao Qiang , Yang Fusun , He Chaozhu , Zhang Liangsheng , Wan Yinglang 
Published: September 2022

Summary

The areca palm (Areca catechu) has a monoecious spadix, with male flowers on the apical side and females on the basal side. Here, we applied multiomics analysis to investigate sex determination and floral organ development in areca palms.

We generated a chromosome‐level reference genome of A. catechu with 16 pseudochromosomes, composed of 2.73 Gb and encoding 31 406 genes. Data from RNA‐seq and ATAC‐seq (assay for transposase accessible chromatin sequencing) suggested that jasmonic acid (JA) synthesis and signal transduction‐related genes were differentially expressed between female and male flowers via epigenetic modifications.

JA concentration in female flowers was c. 10 times than that in males on the same inflorescence, while JA concentration in hermaphroditic flowers of abnormal inflorescences was about twice that in male flowers of normal inflorescences. JA promotes the development of female flower organs by decreasing the expression of B‐function genes, including AGL16, AP3, PIb and PIc.

There is also a region on pseudochromosome 15 harboring sex‐related genes, including CYP703, LOG, GPAT, AMS and BiP. Among them, CYP703, AMS and BiP were specifically expressed in male flowers.
Arbuscular mycorrhizal fungi influence host infection during epidemics in a wild plant pathosystem
Authors: Eck Jenalle L. , Kytöviita Minna‐Maarit , Laine Anna‐Liisa 
Published: September 2022

SummaryWhile pathogenic and mutualistic microbes are ubiquitous across ecosystems and often co‐occur within hosts, how they interact to determine patterns of disease in genetically diverse wild populations is unknown.To test whether microbial mutualists provide protection against pathogens, and whether this varies among host genotypes, we conducted a field experiment in three naturally occurring epidemics of a fungal pathogen,Podosphaera plantaginis, infecting a host plant,Plantago lanceolata, in the Åland Islands, Finland. In each population, we collected epidemiological data on experimental plants from six allopatric populations that had been inoculated with a mixture of mutualistic arbuscular mycorrhizal fungi or a nonmycorrhizal control.Inoculation with arbuscular mycorrhizal fungi increased growth in plants from every population, but also increased host infection rate. Mycorrhizal effects on disease severity varied among host genotypes and strengthened over time during the epidemic. Host genotypes that were more susceptible to the pathogen received stronger protective effects from inoculation.Our results show that arbuscular mycorrhizal fungi introduce both benefits and risks to host plants, and shift patterns of infection in host populations under pathogen attack. Understanding how mutualists alter host susceptibility to disease will be important for predicting infection outcomes in ecological communities and in agriculture.