bluuebunny/crossref_metadata_2025_split huggingface dataset:
What's behind your eosinophilic myocarditis? A case of Churg–Strauss syndrome diagnosed during acute heart failure
Authors: Amelotti Nicola , Mapelli Massimo , Guglielmo Marco , Pires Maria Inês Fiuza Branco , Campodonico Jeness , Majocchi Benedetta , Ribatti Valentina , Vettor Giulia , Baggiano Andrea , Catto Valentina , Basso Cristina , Pontone Gianluca , Agostoni Piergiuseppe 
Published: October 2022

AbstractEosinophilic granulomatosis with polyangiitis (EGPA) is a rare multisystem disorder; cardiac involvement may include eosinophilic myocarditis. A 67‐year‐old woman presented with 1‐week history of dyspnoea and orthopnoea. She had a history of adult‐onset asthma and peripheral eosinophilia. The investigations showed T‐wave inversion on lateral leads, peripheral eosinophilia, elevated troponin and BNP values, and severe biventricular systolic dysfunction with diffuse hypokinesia and apical akinesia. Computed tomography excluded coronary disease and showed bilateral basal ground‐glass opacities, air‐space consolidation, and bilateral reticular‐nodular pattern. Cardiac magnetic resonance findings were compatible with active myocardial inflammation. An endomyocardial biopsy (EMB) confirmed the diagnosis of eosinophilic myocarditis, and a therapy with oral corticosteroids and heart failure medications was started.
Effects of a one‐time application of controlled‐release nitrogen fertilizer on quality and yield of rice
Authors: Dong Xu , Ying Zhu , Haibin Zhu , Qun Hu , Guodong Liu , Haiyan Wei , Hongcheng Zhang 
Published: October 2022

AbstractLimited information is available on the influence of a one‐time application of controlled‐release nitrogen fertilizer (CRNF) in improving both yield and quality of rice. In this study, two rice cultivars, Jia58 (J58) and Jia67(J67), were used to study the effects of six combinations of short‐acting (40–60‐day release periods) and a long‐acting (80–120‐day release periods) CRNF. The six combinations were as follows: A1, 40‐day + 80‐day CRNFs; A2, 40‐day + 100‐day CRNFs; A3, 40‐day + 120‐day CRNFs; B1, 60‐day + 80‐day CRNFs; B2, 60‐day + 100‐day CRNFs; and B3, 60‐day + 120‐day CRNFs. Each combination of CRNFs was mixed with common urea (CU) to apply as basal fertilizer to rice crops to assess grain yield and quality. In addition, CU without CRNFs was applied as the control (CK). The results showed that the release of nitrogen from A2 and B2 were most matched with nitrogen accumulation by rice resulting in a higher yield (B2 treatment) or the same yield (A2 treatment) as CK. The average yield of 2 years of J58 in B2 was 7.38% higher than that of the CK. Similarly, the average yield of J67 in B2 was 6.46% higher than CK for both years. Milling quality, appearance quality, milled rice starch content, and taste value of rice in CRNF treatments were significantly higher than CK. In contrast, protein, gliadin, and glutenin contents and hardness of rice in the CRNF treatments were lower than CK. The higher protein content of rice in the CK resulted in higher gelatinization temperature and harder rice, which may explain why the eating and cooking qualities of rice in the CK were lower than those of rice in the CRNF treatment. These results demonstrate that the A2 and B2 treatments can produce improvements in both yield and quality of rice.
Extensions of the Lee–Carter model to project the data‐driven rotation of age‐specific mortality decline and forecast coherent mortality rates
Authors: Liu Cuixia , Shi Yanlin 
Published: October 2022

AbstractAccurate forecasts and analyses of mortality rates are essential to many operational practices, such as the designing of pension schemes. Recent studies have focused on the age coherent forecasts, such that long‐term predictions will not diverge infinitely among ages. Although intuitively and biologically reasonable, this age coherence property is lost when the seminal Lee–Carter (LC) model and almost all its existing extensions are employed. Those include the famous Li–Lee (LL) model, which is argued to produce coherent forecasts of mortality rates across populations, but not ages. In this paper, we progressively explore two effective frameworks with three extensions, by applying the LL specification to the single‐population model (LC‐S) and by introducing time‐dependent age patterns of mortality declines in the forecasting steps with fixed (LC‐VF) and variant (LC‐V) intercepts when modeling those patterns. In particular, the recommended LC‐V model is motivated from the intermediate LC‐S and LC‐VF and resolves all remaining issues of both models. Also, the final LC‐V model is easy to implement, incorporates the dynamic and rotating age‐specific patterns of mortality decline, provides age‐coherent forecasts of mortality rates in the long run, and is straightforwardly extensible to multi‐population cases. Using a large sample of 15 countries, we show that the LC‐V model and its multipopulation counterpart can consistently improve the forecasting accuracy of the competing LC, LL and other extensions in both single‐population and multipopulation scenarios. Long‐term analyses up to 2100 further demonstrate the existence of age coherence when forecasting with the proposed LC‐V model.
X‐Ray Crystallographic Studies of Quasi‐Racemates for Absolute Configuration Determinations
Authors: Wu Xingxing , Malinčík Juraj , Prescimone Alessandro , Sparr Christof 
Published: October 2022

AbstractThe determination of the absolute configuration of chiral molecular entities by means of X‐ray crystallography is overall of central importance in stereochemistry. However, the growth of enantiopure single crystals often represents an unsurmountable and frustrating hurdle. Quasi‐racemic crystals of biomacromolecules, for which the absolute configuration is predetermined by the chiral monomers, allowed the X‐ray crystallographic analysis of systems that are difficult to crystallize as pure enantiomers, with aims other than the determination of the absolute structure. Taking advantage of the greater propensity of quasi‐racemic mixtures to co‐crystallize compared to growing enantiomerically pure crystals of a single compound, we herein describe the use of X‐ray crystallography of quasi‐racemates for the absolute configuration determination. We expect that this approach is particularly useful to establish the sense of selectivity in the development of stereoselective methods by simplified crystallizations, while confirming the consistent selectivity with a second molecular structure within the same measurement.
Adding a Chemical Biology Twist to CRISPR Screening
Authors: Huang Shiying , Baskin Jeremy M. 
Published: October 2022

AbstractIn less than a decade, CRISPR screening has revolutionized forward genetics and cell and molecular biology. Advances in screening technologies, including sgRNA libraries, Cas9‐expressing cell lines, and streamlined sequencing pipelines, have democratized pooled CRISPR screens at genome‐wide scale. Initially, many such screens were survival‐based, identifying essential genes in physiological or perturbed processes. With the application of new chemical biology tools to CRISPR screening, the phenotypic space is no longer limited to live/dead selection or screening for levels of conventional fluorescent protein reporters. Further, the resolution has been increased from cell populations to single cells or even the subcellular level. We highlight advances in pooled CRISPR screening, powered by chemical biology, that have expanded phenotypic space, resolution, scope, and scalability as well as strengthened the CRISPR/Cas enzyme toolkit to enable biological hypothesis generation and discovery.
Interleukin‐11/gp130 upregulates MMP‐13 expression and cell migration in OSCC by activating PI3K/Akt and AP‐1 signaling
Authors: Wu Chia‐Yu , Liu Ju‐Fang , Tsai Hsiao‐Chi , Tzeng Huey‐En , Hsieh Tsung‐Han , Wang Ming , Lin Yu‐Feng , Lu Chien‐Chi , Lien Ming‐Yu , Tang Chih‐Hsin 
Published: October 2022

AbstractOral squamous cell carcinoma (OSCC) is an extremely common head and neck cancer with a poor 5‐year survival rate, especially in cases of metastatic disease. Interleukin (IL)‐11 reportedly promotes cell growth and the epithelial–mesenchymal transition process in metastasis. However, the molecular mechanisms of IL‐11 in OSCC metastasis are unclear. This study found that IL‐11 upregulates matrix metalloproteinase 13 (MMP‐13) expression in OSCC via the IL‐11 receptor alpha subunit/glycoprotein 130 receptors that activate phosphatidyl‐inositol 3‐kinase, Ak strain transforming, and activator protein 1 signaling, which subsequently enhance MMP‐13‐induced tumor metastasis. TIMER2.0 analysis revealed a positive correlation between MMP‐13 and IL‐11 levels (r = 0.454). Moreover, a strong positive association was observed between higher levels of IL‐11 expression in OSCC tissue (p < 0.01), lymph node metastasis (p = 0.0154), and clinical disease stage (p = 0.0337). IL‐11 knockdown suppressed the migration of OSCC cells (p < 0.05). The evidence indicates that IL‐11 can serve as a new molecular therapeutic target in OSCC metastasis.
Comparative evaluation of the antifungal efficacy of sodium hypochlorite, chlorhexidine, and silver nanoparticles against Candida albicans
Authors: Oncu Aysenur , Celikten Berkan , Aydın Betül , Amasya Gulin , Açık Leyla , Sevimay Fatma Semra 
Published: October 2022

AbstractCandida albicans is a microorganism that causes root canal infections. However, it cannot be eliminated with conventional irrigation solutions. Recently, silver nanoparticles (AgNPs) have become popular for their superior antimicrobial effects. The purpose of this study was to evaluate the antifungal effect of AgNPs to C. albicans comparing with 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX). Silver nanoparticles were synthesized by chemical reduction method. Minimum inhibitory concentration and minimum fungicidal concentration of AgNPs against C. albicans strain were determined according to microdilution method reported by Clinical Laboratory Standards Institute. C. albicans biofilm layer was formed on the dentin blocks for 10 days. The biofilm structure was observed by scanning electron microscopy. Statistical analysis was performed with one way analysis of variance analysis and group comparisons were performed with Tukey test. AgNPs showed the highest antifungal effect among the groups. 5.25% NaOCl showed the lowest antifungal effect among the groups. While the 2% CHX solution had a statistically lower antifungal effect than AgNPs, it was found to have a higher effect than NaOCl (p < .016). Nanoparticles present a wide research field as an alternative irrigation solution in root canal treatment. The antifungal effect of AgNPs against C. albicans was confirmed in this study. Further in vivo studies should evaluate the conditions of use and long‐term prognosis of AgNPs.
How should we address the inevitable harms from non‐negligent variant reclassification in predictive genetic testing?
Authors: Rashkin Misha , Kingham Kerry , Lara‐Otero Karlena , Mckenna Meghan , Villiers Janelle , Worthington Monty Mykolas , Prince Anya 
Published: October 2022

AbstractThe process of interpreting genetic variants, in which experts use all available evidence to determine whether an identified variant is associated with a current or future disease, is both scientific and nevertheless subjective. In this paper, we summarize the existing evidence that any given variant could be reclassified and that such a reclassification could lead to harm. Furthermore, the racial gap in genetic databases could lead to a higher likelihood of harm for non‐white patients. We also review recent legal analyses indicating it is unlikely that an individual who sues for restitution would be successful, especially in the absence of evidence of lab negligence. We then propose a compensation program for medical genetic tests to ensure that individuals who experience demonstrable harm due to a variant reclassification can be made whole financially. We conclude by discussing outstanding questions that must be answered for such a program to be feasible.
Factor structure of the 10‐item CES‐D Scale among patients with persistent COVID‐19
Authors: Ramírez‐Vélez Robinson , Olabarrieta‐Landa Laiene , Rivera Diego , Izquierdo Mikel 
Published: October 2022

AbstractThe presence of persistent coronavirus disease 2019 (COVID‐19) might be associated with significant levels of psychological distress that would meet the threshold for clinical relevance. The Center for Epidemiologic Studies Depression Scale (CES‐D) version 10 has been widely used in assessing psychological distress among general and clinical populations from different cultural backgrounds. To our knowledge, however, researchers have not yet validated these findings among patients with persistent COVID‐19. A cross‐sectional validation study was conducted with 100 patients from the EXER‐COVID project (69.8% women; mean (±standard deviation) ages: 47.4 ± 9.5 years). Confirmatory factor analyses (CFAs) were performed on the 10‐item CES‐D to test four model fits: (a) unidimensional model, (b) two‐factor correlated model, (c) three‐factor correlated model, and (d) second‐order factor model. The diagonal‐weighted least‐squares estimator was used, as it is commonly applied to latent variable models with ordered categorical variables. The reliability indices of the 10‐item CES‐D in patients with persistent COVID‐19 were as follows: depressive affect factor (; ), somatic retardation factor (; ), and positive affect factor (; ). The second‐order model fit showed good Omega reliability (). Regarding CFAs, the unidimensional‐factor model shows poor goodness of fit, especially residuals analysis (root mean square error of approximation [RMSEA] = 0.081 [95% confidence interval, CI = 0.040–0.119]; standardized root mean square residual [SRMR] = 0.101). The two‐factor correlated model, three‐factor correlated model, and second‐order factor model showed adequate goodness of fit, and the χ2 difference test () did not show significant differences between the goodness of fit for these models (; p = 0.127). Several indices showed a good fit with the three‐factor correlated model: goodness‐of‐fit index = 0.974, comparative fit index = 0.990, relative noncentrality index = 0.990, and incremental fit index = 0.990, which were all above 0.95, the traditional cut‐off establishing adequate fit. On the other hand, RMSEA = 0.049 (95% CI = 0.000–0.095), where an RMSEA < 0.06–0.08 indicates an adequate fit. Item loadings on the factors were statistically significant (; p's < 0.001), indicating that the items loaded correctly on the corresponding factors and the relationship between factors (; p's ≤ 0.001. To our knowledge, this is the first study to provide validity and reliability to 10‐item CES‐D in a persistent COVID‐19 Spanish patient sample. The validation and reliability of this short screening tool allow us to increase the chance of obtaining complete data in a particular patient profile with increased fatigue and brain fog that limit patients' capacity to complete questionnaires.
Multiple sampling methods to develop indices of mid‐trophic levels abundance in open ocean ecosystems
Authors: Escobar‐Flores Pablo C. , Décima Moira , O'Driscoll Richard L. , Ladroit Yoann , Roberts Jim 
Published: October 2022

AbstractMid‐trophic level (MTL) organisms play a key role in the sub‐Antarctic ecosystem food web, linking primary producers and tertiary consumers transferring energy across trophic levels. In this region, the relative abundance of MTLs has been monitored using single‐frequency acoustic data collected opportunistically in a time series of trawl surveys carried out in the austral late spring/early summer. To advance this approach, we developed a methodology that enabled us to disaggregate multifrequency acoustic data into three different MTL groups to assess their temporal and spatial patterns: mesopelagic fish, gelatinous zooplankton (excluding pyrosomes), and euphausiids. We used acoustic data and biological sampling information collected with two midwater trawl nets from a dedicated voyage, to train a classification tree algorithm. We complemented these data with footage from an in‐trawl camera system that gave us new insights on vertical distribution and presence of pelagic taxa. The testing dataset used to implement our classification algorithm was a time series of acoustic data from the sub‐Antarctic trawl survey. This approach was successful at disaggregating acoustic data and provided useful information to study the relative abundance and temporal and spatial patterns of pelagic taxa. Our analysis revealed the presence of dense shallow layers of gelatinous zooplankton which showed a statistically significant increasing trend over the time series. Although we were unable to detect pyrosomes acoustically, we encountered large biovolumes of these organisms during our biological sampling, which was supported by the in‐trawl camera system.
All‐Aqueous, Surfactant‐Free, and pH‐Driven Nanoformulation Methods of Dual‐Responsive Polymer Nanoparticles and their Potential use as Nanocarriers of pH‐Sensitive Drugs
Authors: Lechuga‐Islas Víctor D. , Trejo‐Maldonado Melisa , Anufriev Ilya , Nischang Ivo , Terzioğlu İpek , Ulbrich Jens , Guerrero‐Santos Ramiro , Elizalde‐Herrera Luis E. , Schubert Ulrich S. , Guerrero‐Sánchez Carlos 
Published: October 2022

AbstractAll‐aqueous, surfactant‐free, and pH‐driven nanoformulation methods to generate pH‐ and temperature‐responsive polymer nanoparticles (NPs) are described. Copolymers comprising a poly(methyl methacrylate) (PMMA) backbone with a few units of 2‐(dimethylamino)ethyl methacrylate (DMAEMA) are solubilized in acidic buffer (pH 2.0) to produce pH‐sensitive NPs. Copolymers of different molar mass (2.3–11.5 kg mol−1) and DMAEMA composition (7.3–14.2 mol%) are evaluated using a “conventional” pH‐driven nanoformulation method (i.e., adding an aqueous polymer solution (acidic buffer) into an aqueous non‐solvent (basic buffer)) and a robotized method for pH adjustment of polymer dispersions. Dynamic light scattering, zeta‐potential (ζ), and sedimentation‐diffusion analyses suggest the formation of dual‐responsive NPs of tunable size (from 20 to 110 nm) being stable for at least 28 days in the pH and temperature intervals from 2.0 to 6.0 and 25 to 50 °C, respectively. Ultraviolet‐visible spectroscopic experiments show that these NPs can act as nanocarriers for the pH‐sensitive dipyridamole drug, expanding its bioavailability and potential controlled release as a function of pH and temperature. These approaches offer alternative strategies to prepare stimuli‐responsive NPs, avoiding the use of harmful solvents and complex purification steps, and improving the availability of biocompatible polymer nanoformulations for specific controlled release of pH‐sensitive cargos.
Using representational and abstract imagery to create regulatory fit effects
Authors: Naletelich Kelly , Ketron Seth , Spears Nancy , Gelves J. Alejandro 
Published: October 2022

AbstractVisual imagery is one of the most important methods of communicating with consumers, but scholars have generally neglected the role of different forms of visual imagery (representational and abstract). Further, the motivation to engage with and process representational versus abstract imagery has also remained underexamined despite the important role that motivation, particularly stemming from regulatory focus, plays in the consumer domain. Therefore, we demonstrate that prevention‐focused versus promotion‐focused mindsets guide the interpretation of meanings conveyed by representational versus abstract visual imagery as a nonverbal means to achieve regulatory fit. Four experimental studies— including one controlled laboratory experiment and one online behavioral response study—show that when representational imagery is matched with a prevention‐focused and abstract imagery with a promotion‐focused mindset or framed message, consumer outcomes are enhanced. Further, we find that perceived risk mediates the results for those with a prevention focus and departure from the status quo for those with a promotion focus.
Gene polymorphisms of cyclin‐dependent kinase inhibitor and matrix metalloproteinase‐9 in Sudanese patients with esophageal squamous cell carcinoma
Authors: Eltayeb Majdolin Mohammed , Ali Mohamed M. , Omar Saeed M. , Mohamed Nouh Saad , Adam Ishag , Hamdan Hamdan Z. 
Published: October 2022

AbstractBackgroundThe polymorphisms of the cyclin‐dependent kinase inhibitor (CDKN1A) gene and matrix metalloproteinase‐9 (MMP9) gene may increase one's susceptibility to malignancies. In this study, the association of the single nucleotide polymorphisms (SNPs) CDKN1A rs1059234 c.70C>T at the 3′ untranslated region and MMP9 rs17576 (c.836A>G, p.Gln279Arg) with esophageal squamous cell carcinoma (ESCC) in Sudanese individuals were investigated.Materials and MethodsA case‐control study involving age‐ and gender‐matched groups were conducted in a cancer center in eastern Sudan (Gadarif) between April and October 2020. The case group consisted of ESCC patients, whereas the control group comprised healthy subjects. Polymerase chain reaction‐restriction fragment length polymorphism was performed for the genotyping of the CDKN1A rs1059234 and MMP9 rs17576 SNPs. The genotyping results were confirmed by Sanger sequencing.ResultsThe genotype distributions for CDKN1A rs1059234 and MMP9 rs17576 were in agreement with the Hardy–Weinberg equilibrium. The variant allele T in CDKN1 rs1059234 c.70C>T was significantly more prevalent in the ESCC patients than in the healthy controls [51.3% vs. 19.2%; OR = 4.4; 95% CI (2.6–7.4); p < 0.001]. Moreover, in CDKN1A rs1059234, the genotype TC + TT [76.9% vs. 38.4%; OR = 5.3; 95% CI (2.6–10.7); p < 0.001] was more frequent in the cases than in the controls, and it was significantly associated with ESCC risk. In MMP9 rs17576, the variant allele G was also significantly prevalent in the cases relative to the controls, and it was significantly associated with increased ESCC risk in the cases compared with the controls [27.5% vs. 1.9%; OR = 19.4; 95%CI (5.8–64.1); p < 0.001]. Both genotypes containing the allele G (AG + GG) were the most common genotypes in the cases [48.7% vs. 3.8%; OR = 23.7; 95%CI (6.8–81.7); p < 0.001], and they significantly increased the risk of ESCC.ConclusionA significant increase in ESCC risk is associated with the SNPs CDKN1A rs1059234 and MMP9 rs17576.
Design and demonstration of a 2.5D frequency‐selective surface for sub‐6 GHz 5G wireless communication
Authors: Katoch Kanishka , Jaglan Naveen , Gupta Samir D. , Abdollahvand Mousa , Sharawi Mohammad S. 
Published: October 2022

AbstractThis paper presents an ultraminiaturized bandstop closed‐loop 2.5D frequency‐selective surface (FSS) for sub‐6 GHz 5G wireless communication. Four metallic vertical vias interconnect metallic elements present on both sides of the substrate to form a modified closed‐loop design. The proposed structure band stops the entire sub‐6 GHz 5G band (C‐band/NR N78 band/LTE 42/43 band) ranging from 3.2 to 3.9 GHz with a resonant notch at 3.62 GHz. This design supports polarization insensitivity and procures maximum relative deviation of 0.5% and 0.2% at 80°incident transverse electric (TE) and transverse magnetic (TM) polarized waves, respectively. The proposed FSS prototype has been fabricated and tested. Experimentally, the resonant notch is obtained at 3.55 and 3.68 GHz under TE and TM normal incident polarized waves, respectively. Moreover, good agreement in the frequency response curves is observed between simulated and measured results. The proposed unit cell is 3.6 × 3.6 mm2 in dimensions, which is 0.04λ × 0.04λ, where λ is the resonant wavelength.