bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of the intestinal bacterial community in black soldier fly larval guts and its influence on insect growth and development
Authors: Li Xin‐Yu , Mei Cheng , Luo Xing‐Yu , Wulamu Dilinuer , Zhan Shuai , Huang Yong‐Ping , Yang Hong 
Published: July 2022

AbstractBlack soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is a prominent insect for the bioconversion of various organic wastes. As a saprotrophic insect, the BSF inhabits microbe‐rich environments. However, the influences of the intestinal microorganisms on BSF growth and development are not very clear. In this study, the dynamics of the intestinal bacterial community of BSF larvae (BSFL) were analyzed using pyrosequencing. Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars. The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, and Dysgonomonas were the relatively most abundant bacteria in the 1st‐ to 4th‐instar BSFL, respectively. Dysgonomonas and Providencia also dominated the 5th‐ and 6th‐instar larvae, at ratios of 31.1% and 47.2%, respectively. In total, 148 bacterial strains affiliated with 20 genera were isolated on different media under aerobic and anaerobic conditions. Among them, 6 bacteria, BSF1–BSF6, were selected for further study. The inoculation of the 6 isolates independently into germ‐free BSFL feeding on an artificial diet showed that all the bacteria, except BSF4, significantly promoted BSF growth and development compared with the germ‐free control. Citrobacter, Dysgonomonas, Klebsiella, Ochrobactrum, and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae, as well as increasing the prepupae and eclosion rates. In addition, Citrobacter, Klebsiella and Providencia shortened the BSF life cycle significantly. The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.
Multi‐organs perioperative immune‐related adverse events and postoperative bronchial anastomotic fistula in a patient receiving neoadjuvant immunotherapy with NSCLC
Authors: Xu Yuan , Lyu Xiaohong , Qin Yingzhi , Ma Dongjie , Wang Mengzhao , Shi Juhong , Long Yun , Tang Bo , Liu Hongsheng 
Published: July 2022

AbstractThe safety of neoadjuvant chemoimmunotherapy before surgery in patients with non–small cell lung cancer (NSCLC) remains unclear in the perioperative stage. We describe a case of a 63‐year‐old man with IIIC stage NSCLC who received neoadjuvant chemoimmunotherapy and radical lobectomy. After the second cycle of pembrolizumab and chemotherapy (paclitaxel + carboplatin), the patient was diagnosed with immunologic enterocolitis and relieved by glucocorticoid therapy. Radical lobectomy of the right upper lobe was then performed. On postoperative day 4 (POD 4), the patient suddenly suffered suffocated wheezing during sleep. Interstitial lung disease was, therefore, identified by chest computed tomography scan. Glucocorticoids and mechanical ventilation were applied and the symptoms were relieved. On POD 10, the patient developed a bronchial fistula and underwent emergent repair surgery. This is the first case of multi‐organs, multi‐time point immune‐related adverse events (irAE) in perioperative NSCLC patients who received neoadjuvant chemoimmunotherapy. Clinicians should be on high alert for signs of irAEs in neoadjuvant chemoimmunotherapy patients, promptly requiring multidisciplinary management.
Uniportal thoracoscopic left posterior basal segmentectomy using a posterior approach
Authors: Igai Hitoshi , Matsuura Natsumi , Kamiyoshihara Mitsuhiro 
Published: July 2022

AbstractPosterior basal (S10) segmentectomy is one of the most challenging (and uncommon) types of pulmonary segmentectomy. Here, we present two key tips for facilitating a uniportal operation. The first is a full understanding of the relative locations of the pulmonary vessels and bronchi (as revealed by preoperative three‐dimensional computed tomography/broncho‐angiography), and the other is the use of “suction‐guided stapling” to dissect and divide the peripheral pulmonary vessels and bronchi. We describe the successful postoperative course of a patient who was so treated.
Glycogen accumulation in adipocyte precursors from elderly and obese subjects triggers inflammation via SIRT1/6 signaling
Authors: Terrón‐Puig Margarida , Huber‐Ruano Isabel , Sabadell‐Basallote Joan , Ejarque Miriam , Núñez‐Roa Catalina , Maymó‐Masip Elsa , Jorba Rosa , Serena Carolina , Vendrell Joan , Fernández‐Veledo Sonia 
Published: July 2022

AbstractDysfunctional adipocyte precursors have emerged as key determinants for obesity‐ and aging‐related inflammation, but the mechanistic basis remains poorly understood. Here, we explored the dysfunctional adipose tissue of elderly and obese individuals focusing on the metabolic and inflammatory state of human adipose‐derived mesenchymal stromal cells (hASCs), and on sirtuins, which link metabolism and inflammation. Both obesity and aging impaired the differentiation potential of hASCs but had a different impact on their proliferative capacity. hASCs from elderly individuals (≥65 years) showed an upregulation of glycolysis‐related genes, which was accompanied by increased lactate secretion and glycogen storage, a phenotype that was exaggerated by obesity. Multiplex protein profiling revealed that the metabolic switch to glycogenesis was associated with a pro‐inflammatory secretome concomitant with a decrease in the protein expression of SIRT1 and SIRT6. siRNA‐mediated knockdown of SIRT1 and SIRT6 in hASCs from lean adults increased the expression of pro‐inflammatory and glycolysis‐related markers, and enforced glycogen deposition by overexpression of protein targeting to glycogen (PTG) led to a downregulation of SIRT1/6 protein levels, mimicking the inflammatory state of hASCs from elderly subjects. Overall, our data point to a glycogen‐SIRT1/6 signaling axis as a driver of age‐related inflammation in adipocyte precursors.
Integration analyses of structural variations and differential gene expression associated with beef fatty acid profile in Nellore cattle
Authors: Berton Mariana Piatto , de Lemos Marcos Vinícius Antunes , Stafuzza Nedenia Bonvino , Simielli Fonseca Larissa Fernanda , Silva Danielly Beraldo dos Santos , Peripolli Elisa , Pereira Angélica S. C. , Magalhães Ana Fabricia Braga , Albuquerque Lucia G. , Baldi Fernando 
Published: July 2022

AbstractThis study aimed to integrate analyses of structural variations and differentially expressed genes (DEGs) associated with the beef fatty acid (FA) profile in Nellore cattle. Copy numbers variation (CNV) detection was performed using the penncnv algorithm and CNVRuler software in 3794 genotyped animals through the High‐Density Bovine BeadChip. In order to perform the genomic wide association study (GWAS), a total of 963 genotyped animals were selected to obtain the intramuscular lipid concentration and quantify the beef FA profile. A total of 48 animals belonging to the same farm and management lot were extracted from the 963 genotyped and phenotyped animals to carry out the transcriptomic and differentially expressed gene analyses. The GWAS with extreme groups of FA profiles was performed using a logistic model. A total of 43, 42, 66 and 35 significant CNV regions (p < 0.05) for saturated, monounsaturated, polyunsaturated and omega 3 and 6 fatty acids were identified respectively. The paired‐end sequencing of 48 samples was performed using the Illumina HiSeq2500 platform. Real‐time quantitative PCR was used to validate the DEGs identified by RNA‐seq analysis. The results showed several DEGs associated with the FA profile of Longissimus thoracis, such as BSCL2 and SAMD8. Enriched terms as the cellular response to corticosteroid (GO:0071384) and glucocorticoid stimulus (GO:0071385) could be highlighted. The identification of structural variations harboring candidate genes for beef FA must contribute to the elucidation of the genetic basis that determines the beef FA composition of intramuscular fat in Nellore cattle. Our results will contribute to the identification of potential biomarkers for complex phenotypes, such as the FA profile, to improve the reliability of the genomic predictions including pre‐selected variants using differentiated weighting in the genomic models.
Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium
Authors: Himelreich Perić Marta , Mužić‐Radović Vedrana , Marić Tihana , Bulić‐Jakuš Floriana , Jurić‐Lekić Gordana , Takahashi Marta , Sinčić Nino , Ježek Davor , Katušić‐Bojanac Ana 
Published: July 2022

AbstractCartilage differentiates in rat limb buds cultivated in a chemically defined protein‐free medium in the same manner as in the richer serum‐supplemented medium. We aimed to investigate the remaining differentiation potential of pre‐cultivated limb buds by subsequent transplantation in vivo. Rat front (FLBs) and hind‐limb buds (HLBs) were isolated from Fischer rat dams at the 14th gestation day (GD 14) and cultivated at the air‐liquid interface in Eagle's Minimum Essential Medium (MEM) alone; with 5 μM of 5‐azacytidine (5azaC) or with rat serum (1:1). Overall growth was measured seven times during the culture by an ocular micrometre. After 14 days, explants were transplanted under the kidney capsule of adult males. Growth of limb buds was significantly lower in all limb buds cultivated in MEM than in those cultivated with serum. In MEM with 5azaC, growth of LBs was significantly lower only on day 3 of culture. Afterwards, it was higher throughout the culture period, although a statistically significant difference was assessed only for HLBs. In transplants, mixed structures developed with the differentiated transmembranous bone, cartilage with enchondral ossification, bone‐marrow, sebaceous gland, and hair that have never been found in vitro. Nerves differentiated only in transplants precultivated in the serum‐supplemented medium. We conclude that pre‐cultivation of LBs in a chemically defined protein‐free medium does not restrict osteogenesis and formation of epidermal appendages but is restrictive for neural tissue. These results are important for understanding limb development and regenerative medicine strategies.
Fracture liaison service utilising an emergency department information system to identify patients effectively reduce re‐fracture rate is cost‐effective and cost saving in Western Australia
Authors: Inderjeeth Charles A. , Raymond Warren D. , Geelhoed Elizabeth , Briggs Andrew M. , Oldham David , Mountain David 
Published: July 2022

AbstractObjectivesTo assess the benefits of the Emergency Department Information System (EDIS)‐linked fracture liaison service (FLS).MethodsPatients identified through EDIS were invited to attend an FLS at the intervention hospital, the Sir Charles Gairdner Hospital (SCGS‐FLS). The intervention group was compared to usual care. Retrospective control (RC) at this hospital determined historical fracture risk (SCGH‐RC). Prospective control (PC) was from a comparator, Fremantle Hospital (FH‐PC). The main outcome measures were cost‐effectiveness from a health system perspective and quality of life by EuroQOL (EQ‐5D). Bottom‐up cost of medical care, against the cost of managing recurrent fracture (weighted basket), was determined from the literature and 2013/14 Australian Refined Diagnosis Related Groups (AR‐DRG) prices. Mean incremental cost‐effectiveness ratios were simulated from 5000 bootstrap iterations. Cost‐effectiveness acceptability curves were generated.ResultsThe SCGH‐FLS program reduced absolute re‐fracture rates versus control cohorts (9.2–10.2%), producing an estimated cost saving of AUD$750,168–AUD$810,400 per 1000 patient‐years in the first year. Between‐groups QALYs differed with worse outcomes in both control groups (p < 0.001).The SCGH‐FLS compared with SCGH‐RC and FH‐PC had a mean incremental cost of $8721 (95% CI −$1218, $35,044) and $8974 (95% CI −$26,701, $69,929), respectively, per 1% reduction in 12‐month recurrent fracture risk. The SCGH‐FLS compared with SCGH‐RC and FH‐PC had a mean incremental cost of $292 (95% CI −$3588, $3380) and −$261 (95% CI −$1521, $471) per EQ‐5D QALY gained at 12 months respectively. With societal willingness to pay of $16,000, recurrent fracture is reduced by 1% in >80% of patients.ConclusionsThis simple and easy model of identification and intervention demonstrated efficacy in reducing rates of recurrent fracture and was cost‐effective and potentially cost saving.
Current clinical practice in corneal crosslinking for treatment of progressive keratoconus in four Nordic countries
Authors: Gustafsson Ingemar , Vicente André , Bergström Anders , Stenevi Ulf , Ivarsen Anders , Hjortdal Jesper Østergaard 
Published: July 2022

AbstractPurposeTo evaluate clinical practice in the diagnosis and treatment of progressive keratoconus with corneal crosslinking (CXL) in four Nordic countries.MethodsA questionnaire was sent to all centres at which keratoconus patients are evaluated and CXL is performed in Sweden, Denmark, Norway and Iceland. Nineteen of 20 centres participated.ResultsCXL is performed approximately 1300 times per year in these four Nordic countries with a population of around 21.7 million (2019). In most cases, progression is evaluated using the Pentacam HR, and the maximum keratometry reading (Kmax) is considered the most important parameter. The most frequently used treatment protocol in Scandinavia is the 9 mW/cm2 epi‐off protocol, using hydroxylpropyl methylcellulose riboflavin (HPMC‐riboflavin). The participants deemed the following areas to be in most need of improvement: adaptation of the CXL protocol to individual patients (5/19), the development of effective epi‐on treatment protocols (4/19), optimal performance of CXL in thin corneas (4/19), improvement of the definition of progression (2/19), and diagnosis of the need for re‐treatment (2/19).ConclusionsWe concluded that the diagnosis of progressive keratoconus and the diagnostic equipment used are similar. Treatment strategies are also similar but are suitably different to provide an interesting basis for the comparison of treatment outcomes. The high degree of participation in this survey indicates the possibility of future scientific collaboration on CXL focusing on the areas deemed to need improvement. It would also be of interest to evaluate the possibility of creating a Nordic CXL Registry. The high number of CXL treatments performed ensures sufficient statistical power to solve many questions. Such a registry could be an important contribution to evidence‐based care and would allow for longitudinal evaluation.
Production centers of cooking pots in Iron Age Judah
Authors: Ben‐Shlomo David , Bouzaglou Liora , Mommsen Hans , Sterba Johannes H. 
Published: July 2022

AbstractThis paper will present and discuss a multifaceted research project dealing with the production of cooking pots during the Iron Age II (ca. 1,000–586 BCE) Judah (modern Israel). In particular the new compositional analysis of 541 cooking vessels from 11 sites in Iron Age Judah will be presented. The study employs petrographic and chemical (NAA) analysis. The results of this ongoing research have already produced interesting information about production centers and movements of cooking pots in Iron Age II Judah. Apparently, the vast majority of the cooking pots sampled were made of a similar type of clay, related to terra rossa soil. This is true also for sites in the northern Negev and Judean Desert, where the type of soil was not available in the region of the sites. Furthermore, many of the cooking pots distributed around Judah were made in Jerusalem according to a well‐located chemical profile (JleB). Other groups may represent Judean Shephelah production centers as the Lachish area as well as production centers in southern Israel or ancient Edom. The implications of the importation patterns of cooking pots by peripheral Judean sites will be discussed.
Amiodarone/N‐desethylamiodarone population pharmacokinetics in paediatric patients
Authors: Lehnert Amélia , Foissac Frantz , Bouazza Naïm , Urien Saïk , Oualha Mehdi , Renolleau Sylvain , Barbanti Claudio , Di Marzio Anna , Bonnet Damien , Abdalla Seef , Zheng Yi , Treluyer Jean‐Marc 
Published: July 2022

The population pharmacokinetics of amiodarone and its active metabolite, N‐desethylamiodarone (DEA) were investigated in paediatric patients with arrhythmias, mainly supraventricular tachycardias. A total of 55 patients from the Department of Pediatric Intensive Care and Pediatric Cardiology at Necker‐Enfants malades Hospital (Paris, France) provided 72 concentrations for both amiodarone and DEA following repeated oral or intravenous administration. Blood samples drawn for biological analyses were used for drug concentrations. Plasma amiodarone concentrations were measured by a liquid chromatography method coupled with mass spectrometry and the data were modelled using the software Monolix 2019R2. Parent pharmacokinetics was described with a 2‐compartment open model and the metabolite formation was connected to the central parent compartment. Parameter estimates scaled allometrically on bodyweight (normalized to 70 kg) were, respectively (% relative standard errors, RSEs), 6.32 (31%) and 7.14 L/h (26%) for elimination (CL) and intercompartmental clearances and 167 (31%) and 3930 (32%) L for V1 and V2. Oral bioavailability was 0.362 (21.5%). The clearance between subject variability (ω, square root of the variance) was 0.462 (RSE 21%). The proportional residual variabilities were respectively 0.453 (RSE 13%) and 0.423 (RSE 12%) for amiodarone and DEA respectively. The terminal half‐lives were 34 and 14.5 days for amiodarone and DEA, respectively. A dosage schedule was established for 3 weight bands in 2 time periods. The high pharmacokinetic variability suggests that therapeutic drug monitoring might be useful to improve individual efficacy and safety.
Molecular landscape of IDH‐wild type, pTERT‐wild type adult glioblastomas
Authors: Liu Emma Munan , Shi Zhi‐Feng , Li Kay Ka‐Wai , Malta Tathiane M. , Chung Nellie Yuk‐Fei , Chen Hong , Chan Janice Yuen‐Tung , Poon Manix Fung‐Man , Kwan Johnny Sheung‐Him , Chan Danny Tat‐Ming , Noushmehr Houtan , Mao Ying , Ng Ho‐Keung 
Published: July 2022

AbstractTelomerase reverse transcriptase (TERT) promoter (pTERT) mutation has often been described as a late event in gliomagenesis and it has been suggested as a prognostic biomarker in gliomas other than 1p19q codeleted tumors. However, the characteristics of isocitrate dehydrogenase (IDH) wild type (wt) (IDHwt), pTERTwt glioblastomas are not well known. We recruited 72 adult IDHwt, pTERTwt glioblastomas and performed methylation profiling, targeted sequencing, and fluorescence in situ hybridization (FISH) for TERT structural rearrangement and ALT (alternative lengthening of telomeres). There was no significant difference in overall survival (OS) between our cohort and a the Cancer Genome Atlas (TCGA) cohort of IDHwt, pTERT mutant (mut) glioblastomas, suggesting that pTERT mutation on its own is not a prognostic factor among IDHwt glioblastomas. Epigenetically, the tumors clustered into classic‐like (11%), mesenchymal‐like (32%), and LGm6‐glioblastoma (GBM) (57%), the latter far exceeding the corresponding proportion seen in the TCGA cohort of IDHwt, pTERTmut glioblastomas. LGm6‐GBM‐clustered tumors were enriched for platelet derived growth factor receptor alpha (PDGFRA) amplification or mutation (p = 0.008), and contained far fewer epidermal growth factor receptor (EGFR) amplification (p < 0.01), 10p loss (p = 0.001) and 10q loss (p < 0.001) compared with cases not clustered to this group. LGm6‐GBM cases predominantly showed ALT (p = 0.038). In the whole cohort, only 35% cases showed EGFR amplification and no case showed combined chromosome +7/−10. Since the cases were already pTERTwt, so the three molecular properties of EGFR amplification, +7/−10, and pTERT mutation may not cover all IDHwt glioblastomas. Instead, EGFR and PDGFRA amplifications covered 67% and together with their mutations covered 71% of cases of this cohort. Homozygous deletion of cyclin dependent kinase inhibitor 2A (CDKN2A)/B was associated with a worse OS (p = 0.031) and was an independent prognosticator in multivariate analysis (p = 0.032). In conclusion, adult IDHwt, pTERTwt glioblastomas show epigenetic clustering different from IDHwt, pTERTmut glioblastomas, and IDHwt glioblastomas which are pTERTwt may however not show EGFR amplification or +7/−10 in a significant proportion of cases. CDKN2A/B deletion is a poor prognostic biomarker in this group.
Challenging assumptions of sediment routing in retroarc foreland basins: Detrital zircon evidence for axial versus transverse drainages in the Late Cretaceous of southern Utah, USA
Authors: St. Pierre Gabriela A. Enriquez , Johnson Cari L. 
Published: July 2022

AbstractDepositional models for retroarc foreland basin systems commonly imply a direct link between thrust belt dynamics and sediment supply, despite documented complexity between orogen‐transverse and orogen‐parallel (axial) sediment routing systems. Previous detrital zircon provenance studies from the Straight Cliffs Formation in the Kaiparowits Plateau of southern Utah indicate primary axial source areas to the south (Mogollon Highlands), and southwest (Cordilleran magmatic arc), with relatively minor transverse input from the Sevier fold‐thrust belt to the west. Complementary data from 32 new samples are presented to investigate whether these trends persist in correlated strata from sections most proximal to the active fold‐thrust belt, as well as strata proximal to the Mogollon Highlands. This study aims to improve statistical robustness through higher‐n datasets (>300 analyses/sample) and addresses the possibility of recycling of zircon grains from strata in the exhumed Sevier fold‐thrust belt. Based on this provenance dataset, we infer that orogen‐transverse deposition was mainly limited to the most proximal sections (within ca. 75 km of the fold‐thrust belt), but included episodic distribution across the foredeep that was associated with distinct intervals of widespread amalgamated sand and gravel sheet deposits. In contrast, more distal parts of the foredeep record long‐distance fluvial transport (>400 km) of zircons from the active Cordilleran magmatic arc as well as Yavapai‐Mazatzal basement rocks in central Arizona (the Mogollon Highlands). Palaeogeographic reconstructions suggest the apex of a large basin‐axial fluvial system may have been located at the structural syntaxis between the Sevier fold‐thrust belt and western margin of the Mogollon Highlands. This interpretation is supported by detrital zircon data presented here, thus extending the possibility that the Palaeogene ‘California River’ may have existed by at least Late Cretaceous time. Ultimately, these data point to extrabasinal controls on sediment supply, which was largely decoupled from flexural accommodation controls in this archetype retroarc foreland basin.