bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desfecho clínico de pacientes colonizados e infectados por bactérias multirresistentes em hospital privado de Fortaleza/Ceará, em 2021
Authors: Lima Cicero Allan Landim de Oliveira , Moreira Ana Lívia Gomes , Mesquita Rafael Ferreira , Lima Luan Victor Almeida , Aquino Bruno Pinheiro , Medeiros Melissa Soares 
Published: February 2022

A pandemia pela COVID-19, desde 2019, vem contribuindo para agravar situações que aumentam o risco de desenvolvimento de colonização ou infecção por bactérias multirresistentes, como o uso indiscriminado de antimicrobianos. O objetivo deste estudo foi investigar a relação entre resistência bacteriana e desfecho clínico negativo. Foi realizado estudo do tipo transversal e retrospectivo, onde foram coletados dados dos prontuários de pacientes internados em diferentes unidades hospitalares (UTI, enfermaria, isolamento e neonatologia), de janeiro a dezembro de 2021. Os critérios de inclusão foram os pacientes notificados com colonização ou infecção por bactérias multirresistentes durante o internamento e com dados completos. Foram 128 amostras positivas, com média de idade 68,2 anos. Houve diferença estatisticamente significante em mortalidade ao comparar pacientes com menos de 50 anos e os grupos de faixa etária entre 70-80 anos (p=0,006) e acima de 80 anos (p=0,03). Dentre os três principais patógenos isolados e a elevada taxa de mortalidade dos mesmos, não houve diferença estatisticamente significativa em relação a taxa de mortalidade global para Acinetobacter baumannii (p = 0,54), Klebsiella pneumoniae (p = 1) e Pseudomonas aeruginosa (p = 0,82). Detectou-se resultado estatisticamente significativo para mortalidade e utilização de quinolonas (p = 0,047) e uma tendência a significância para polimixina (p = 0,09). Concluiu-se que houve elevada mortalidade em pacientes colonizados ou infectados por bactérias multirresistentes em âmbito hospitalar, principalmente acima de 70 anos de idade. O uso de quinolonas, aminoglicosídeos e polimixina parece estar relacionado a maior mortalidade.
A humánerőforrási motivációs stratégiák a Dél-dunántúli régió vállalkozásaiban
Authors: Bertalan Péter , Boldizsár Bettina 
Published: February 2022

In Hungary, the economic role of small and medium-sized enterprises (SMEs) is of major importance, however, the sector-specific management techniques are not comparable to those used by larger economic operators. The main objective of the research was to show the presence or lack of motivation as a value creating tool in the management practice of the SME sector in the South Transdanubian Region. A further significance of the research is that it draws attention to management practices that are extremely harmful from an economic point of view, the transformation of which can result in significant profit growth for the companies even without significant financial expenditures. To complement the survey, 15 in-depth management interviews were conducted where 10 questions were analysed. All in all, the executives in the study use management knowledge moderately and only partially consciously in their everyday practice and do not have a systematic motivational strategy. The theory learnt during school years – 15–20 years earlier – sometimes appear in everyday activities, but it is more typical that leaders follow their own paths and rely on information and advice from friends. In either case, management knowledge and motivation practices based on sound information do not play a significant role. Regarding the competencies, self-awareness is hardly recognized. Most executives do not seek regular feedback from their subordinates or give to their staff. Nevertheless, they feel that they are aware of their individual values ​​and systems, especially of those which were mainly built instinctively and experientially. The study showed that the role of innovation in creating a motivating work environment and the extensive economic power of motivation are either not or not fully understood by managers. When describing the ideal worker and their expected qualities, executives did not even mention the necessity of intrinsic motivation!
MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity
Authors: Jaafar S. O. , Jaffar J. O. , Ibrahim S. A. , Jarjees K. K. 
Published: February 2022

Background: While different studies have investigated the association of SNPs with female reproductive disorders, a limited number of studies have investigated the effect of microRNAs variants in endometriosis. In this study, we evaluated the prevalence and the association of three different miRNAs variants including, miR-27a rs895819, miR-124-1 rs531564, and miR-423 rs6505162 with endometriosis to help further elucidate the importance of these variants in female reproductive disorders.Methods: A total number of 440 women (220 cases and 220 controls) were included. DNA was extracted and genotyping of the SNPs was carried out by PCR.Results: The results showed that rs895819 and rs6505162 had a significant association with endometriosis under the dominant, recessive, co-dominant, and allelic model, but rs531564 was not linked to endometriosis. Our results also imply a protective effect on endometriosis severity for AG genotype and G allele in rs895819 (p &lt; 0.001), and also for AA and AC genotypes in rs6505162 with severity in endometriosis (p &lt; 0.001). Moreover, Hardy–Weinberg equilibrium, haplotype frequency, and linkage disequilibrium between SNPs were performed.Conclusion: miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are linked to endometriosis.
Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome
Authors: Li R. , Yu Y. , Jaafar S. O. , Baghchi B. , Farsimadan M. , Arabipour I. , Vaziri H. 
Published: February 2022

Introduction: Alterations in certain microRNAs (miRNAs) and their target genes have reported in polycystic ovary syndrome (PCOS) and other disease of the female reproductive system, and so may be potential biomarkers. We hypothesised alterations in the prevalence of four miRNAs single nucleotide polymorphism (SNP) variants miR-126 rs4636297, miR-146a rs2910164, miR-196a2 rs11614913, and miR-499 rs3746444 in women with PCOS in comparison to healthy controls.Methods: SNPs in the four miRNAs were determined in 385 patients and 385 controls by standard RT-PCR techniques.Results: SNPs in miR-126 and miR-246a were significant linked with PCOS under the allelic, dominant, co-dominant, and recessive models (all p ≤ 0.01). The SNP in miR-499 was linked to PCOS in allelic (T, p = 0.002), dominant (p = 0.035) and recessive (p = 0.003) models. The SNPs -196a was significant linked to PCOS only in the recessive model (p = 0.037). Combining these SNPs in miR-499, mi146a, miR-196a and miR-126 respectively into allele haplotypes found highly significant odds ratios (95% CI) of 0.40 (0.29–0.54) (p &lt; 0.001) for the C-G-C-G haplotype, and 0.46 (0.30–0.70) (p = 0.002) for the C-C-C-A haplotype (p = 0.002) for PCOS.Conclusion: Single SNPs and haplotype combinations in certain SNPs in miR-126, miR-146a, miR-196a2 and miR-499 are strongly linked to PCOS, and so may be useful predictors of this condition.
Effects of Microwave-Assisted Liquid Hot Water Pretreatment on Chemical Composition and Structure of Moso Bamboo
Authors: Cui Jie-Yu , Zhang Ning , Jiang Jian-Chun 
Published: February 2022

The effects of microwave assisted liquid hot water (MA-LHW) pretreatment on the chemical composition of Moso bamboo were investigated, and the fiber structure of pretreated residues were studied. The results showed that MA-LHW pretreatment had high selectivity for the degradation of hemicellulose in Moso bamboo, and the extracted hemicellulose could be used to prepare xylooligosaccharide through enzyme depolymerization. The degradation rates of cellulose and lignin after MA-LHW pretreatment were only 14.73% and 7.18%, which were significantly lower than those of LHW pretreatment; 155.0 mg/g xylobiose and 61.0 mg/g xylotrisoe can be obtained after enzymatic hydrolysis, and the yield of xylo-oligosaccharide reached 80.59% of the theoretical conversion rate. MA-LHW pretreatment increased the removal of hemicellulose, lignin, and other non-crystalline parts in bamboo materials, and more cellulose with crystalline structure was retained, which increased the CrI value of Moso bamboo by 14.84%. FTIR spectra showed that the characteristic peak intensity of hemicellulose was significantly reduced after MA-LHW pretreatment, which confirmed the selective degradation of hemicellulose by MA-LAW pretreatment. Moreover, MA-LHW pretreatment also destroyed O-H, C-H, C-O-C, and β-glucoside bonds in Moso bamboo fiber, caused by the recombination and synthesis of some groups (-CH2 and C=O) of cellulose, hemicellulose, and lignin destroyed under pretreatment conditions.
Metabolic Engineering of Saccharomyces cerevisiae for High-Level Friedelin via Genetic Manipulation
Authors: Gao Hai-Yun , Zhao Huan , Hu Tian-Yuan , Jiang Zhou-Qian , Xia Meng , Zhang Yi-Feng , Lu Yun , Liu Yuan , Yin Yan , Chen Xiao-Chao , Luo Yun-Feng , Zhou Jia-Wei , Wang Jia-Dian , Gao Jie , Gao Wei , Huang Lu-Qi 
Published: February 2022

Friedelin, the most rearranged pentacyclic triterpene, also exhibits remarkable pharmacological and anti-insect activities. In particular, celastrol with friedelin as the skeleton, which is derived from the medicinal plant Tripterygium wilfordii, is a promising drug due to its anticancer and antiobesity activities. Although a previous study achieved friedelin production using engineered Saccharomyces cerevisiae, strains capable of producing high-level friedelin have not been stably engineered. In this study, a combined strategy was employed with integration of endogenous pathway genes into the genome and knockout of inhibiting genes by CRISPR/Cas9 technology, which successfully engineered multiple strains. After introducing an efficient TwOSC1T502E, all strains with genetic integration (tHMG1, ERG1, ERG20, ERG9, POS5, or UPC2.1) showed a 3.0∼6.8-fold increase in friedelin production compared with strain BY4741. Through further double knockout of inhibiting genes, only strains GD1 and GD3 produced higher yields. Moreover, strains GQ1 and GQ3 with quadruple mutants (bts1; rox1; ypl062w; yjl064w) displayed similar increases. Finally, the dominant strain GQ1 with TwOSC1T502E was cultured in an optimized medium in shake flasks, and the final yield of friedelin reached 63.91 ± 2.45 mg/L, which was approximately 65-fold higher than that of the wild-type strain BY4741 and 229% higher than that in ordinary SD-His-Ura medium. It was the highest titer for friedelin production to date. Our work provides a good example for triterpenoid production in microbial cell factories and lays a solid foundation for the mining, pathway analysis, and efficient production of valuable triterpenoids with friedelin as the skeleton.
Prevalence and Prognostic Value of Mesenteric Artery Stenosis in Patients Undergoing Transcatheter Aortic Valve Implantation
Authors: Lu Henri , Rotzinger David , Monney Pierre , Muller Olivier , Egea Michael , Grange Matthieu , Eeckhout Eric , Kirsch Matthias , Qanadli Salah D. 
Published: February 2022

IntroductionData regarding the prevalence of mesenteric artery stenosis in patients undergoing transcatheter aortic valve implantation (TAVI) are scarce. Whether patients with high-risk features for acute mesenteric ischemia (AMesI) have a worse prognosis compared with those without high-risk features is unknown. We aimed to address these questions.MethodsWe included 361 patients who underwent TAVI between 2015 and 2019. Using pre-TAVI computed tomography exams, the number of stenosed arteries in each patient and the degree of stenosis for the coeliac trunk (CTr), SMA and inferior mesenteric artery (IMA) were analyzed. High-risk features for AMesI were defined as the presence of ≥2 arteries presenting with ≥50% stenosis. Patient demographic and echocardiographic data were collected. Endpoints included 30-day all-cause mortality, mortality and morbidity related to mesenteric ischemia.Results22.7% of patients had no arterial stenosis, while 59.3% had 1 or 2 stenosed arteries, and 18.0% presented stenoses in 3 arteries. Prevalence of significant stenosis (≥50%) in CTr, SMA, and IMA were respectively 11.9, 5.5, 10.8%. Twenty patients at high-risk for AMesI were identified: they had significantly higher all-cause mortality (15.0 vs. 1.2%, p &lt; 0.001) and higher mortality related to AMesI (5.0 vs. 0.3%, p = 0.004), compared with non-high-risk patients.ConclusionsPatients at high-risk for AMesI presented with significantly higher 30-day all-cause mortality and mortality related to AMesI following TAVI. Mesenteric revascularization before TAVI interventions may be beneficial in these patients. Prospective studies are needed to clarify these questions.
Mid-Miocene to Present Upper-Plate Deformation of the Southern Cascadia Forearc: Effects of the Superposition of Subduction and Transform Tectonics
Authors: McKenzie Kirsty A. , Furlong Kevin P. , Kirby Eric 
Published: February 2022

The southern Cascadia forearc undergoes a three-stage tectonic evolution, each stage involving different combinations of tectonic drivers, that produce differences in the upper-plate deformation style. These drivers include subduction, the northward migration of the Mendocino triple junction and associated thickening and thinning related to the Mendocino Crustal Conveyor (MCC) effect, and the NNW translation of the Sierra Nevada-Great Valley (SNGV) block. We combine geodetic data, plate reconstructions, seismic tomography and topographic observations to determine how the southern Cascadia upper plate is deforming in response to the combined effects of subduction and NNW-directed (MCC- and SNGV-related) tectonic processes. The location of the terrane boundaries between the relatively weak Franciscan complex and the stronger Klamath Mountain province (KMP) and SNGV block has been a key control on the style of upper-plate deformation in the southern Cascadia forearc since the mid-Miocene. At ∼15 Ma, present-day southern Cascadia was in central Cascadia and deformation there was principally controlled by subduction processes. Since ∼5 Ma, this region of the Cascadia upper plate, where the KMP lies inboard of the Franciscan complex, has been deforming in response to both subduction and MCC- and SNGV-related effects. GPS data show that the KMP is currently moving to the NNW at ∼8–12 mm/yr with little internal deformation, largely in response to the northward push of the SNGV block at its southern boundary. In contrast, the Franciscan complex is accommodating high NNW-directed and NE-directed shortening strain produced by MCC-related shortening and subduction coupling respectively. This composite tectonic regime can explain the style of faulting within and west of the KMP. Associated with this Mendocino Crustal Conveyor crustal thickening, seismic tomography imagery shows a region of low velocity material that we interpret to represent crustal flow and injection of Franciscan crust into the KMP at intracrustal levels. We suggest that this MCC-related crustal flow and injection of material into the KMP is a relatively young feature (post ∼5 Ma) and is driving a rejuvenated period of rock uplift within the KMP. This scenario provides a potential explanation for steep channels and high relief, suggestive of rapid erosion rates within the interior of the KMP.
Evaluation of Bone Mineral Density in Children Conceived via Assisted Reproductive Technology
Authors: Xia Xinru , Chen Lingling , Wang Jing , Yu Xiang , Gao Li , Zhang Yuan , Diao Feiyang , Cui Yugui , Liu Jiayin , Meng Yan 
Published: February 2022

ObjectivesTo investigate bone mineral density (BMD) differences between assisted reproductive technology (ART)-conceived children and naturally conceived (NC) children.Study DesignThis retrospective cohort study included ART-conceived children and controls aged 1 to 12 years assessed with a follow-up protocol. Maternal and paternal background, birth condition, and growth and development indicators were analyzed.ResultsThe ART and NC groups exhibited differences in maternal and paternal childbearing age; maternal weight; maternal body mass index (BMI); maternal alcohol consumption; paternal smoking; delivery method; and serum zinc, iron, and lead levels. Multifactor analysis adjusted for relevant factors showed that paternal childbearing age and group significantly affected the BMD Z score. In the subgroup analysis, in vitro fertilization (IVF) (p=0.026) or intracytoplasmic sperm injection (ICSI) (p=0.008) had a positive impact on the BMD Z score. Male infertility only (p=0.010) or male infertility combined with polycystic ovary syndrome (PCOS) (p=0.026) may affect the BMD Z score. In the embryo transfer cycle subgroup analysis, compared with natural conception, both stimulation cycle fresh embryo transfer (p=0.019) and natural cycle frozen embryo transfer (p=0.006) had a positive effect on the BMD Z score.ConclusionsThe BMD levels of the ART and control groups were generally in the normal range. Paternal childbearing age and the use of ART independently affected the BMD Z score of the offspring.
Field Calibration and Evaluation of an Internet-of-Things-Based Particulate Matter Sensor
Authors: Cowell Nicole , Chapman Lee , Bloss William , Pope Francis 
Published: February 2022

This paper presents a field evaluation of IoT-enabled Plantower PMS5003 particulate matter sensors in Birmingham, United Kingdom. Commercial, off the shelf, sensors were adapted to utilise Low Power Wide Area Network (LPWAN) IoT technology enabling batteries to be used as a power source. The devices are capable of measuring and communicating data to an online platform with a battery life of ∼2 months, at a measurement interval of 15 min, allowing for automated air quality monitoring for extended periods at high density. The sensors demonstrate success at being integrated into a wireless sensor network, with a high presence of readings. The average correlation coefficients (r2) between raw PMS device data and reference instrumentation are 0.718, 0.703, and 0.543 for PM1, PM2.5, and PM10, respectively. The devices also demonstrate good intersensor consistency, with Pearson’s r values between pairs ranging from 0.92 to 0.99 across all size ranges. Relative humidity (RH) clearly influences the response of the sensors, especially for RH &gt;85%, in keeping with previous laboratory evaluations and evaluations of similar devices. The development of a multi-linear correction factor that accounts for humidity effects on the performance of the sensors is described; using this model, Pearson’s r values range from 0.81 to 0.91 compared to 0.73–0.85 from uncorrected values. There is also some evidence of drift at high humidity over an 8-week period, suggesting that such sensors will (at least currently) need recalibration approximately bimonthly. The limit of detection (LoD) (1.60–4.75 μg m−3) calculated from this study also demonstrates that the sensors are suitable for capturing concentrations typical of a moderately polluted United Kingdom urban environment—LoDs of PM2.5 in this study would have allowed for capture of 94.7% of the concentrations recorded at a typical United Kingdom urban roadside monitoring site between 2017 and 2020.
Comprehensive Characterization of Pyroptosis Patterns with Implications in Prognosis and Immunotherapy in Low-Grade Gliomas
Authors: Zhou Zijian , Wei Jinhong , Lu Bin , Jiang Wenbo , Bao Yue , Li Luo , Wang Weimin 
Published: February 2022

Background: Due to high heterogeneity and mortality of low-grade gliomas (LGGs), it is of great significance to find biomarkers for prognosis and immunotherapy. Pyroptosis is emerging as an attractive target in cancer research for its effect on tumor immune microenvironment (TIME). However, the investigation of pyroptosis in LGGs is insufficient.Methods: LGG samples from TCGA and CGGA database were classified into two pyroptosis patterns based on the expression profiles of 52 PRGs using consensus clustering. A prognostic model was constructed by using the LASSO-COX method. ESTIMATE algorithm and single sample gene set enrichment analysis (ssGSEA) were used to characterize the TIME. Based on the differentially expressed genes between two pyroptosis patterns, favorable and unfavorable pyroptosis gene signatures were determined. Pyroptosis score scheme was constructed to quantify the pyroptosis patterns through gene set variation analysis (GSVA) method. Two external datasets and immunotherapy cohort from CGGA and GEO database were used to validate the predictive value of the pyroptosis score. The Human Protein Atlas website and Western blotting were utilized to confirm the expression of the selected genes in the prognostic model in LGGs.Results: Distinct overall survival and immune checkpoint blockage therapeutic responses were identified between two pyroptosis patterns. A low pyroptosis score in LGG patients implies higher overall survival, poor immune cell infiltration, and better response to immunotherapy of immune checkpoint blockage.Conclusion: Our findings provided a foundation for future research targeting pyroptosis and opened a new sight to explore the prognosis and immunotherapy from the angle of pyroptosis in LGGs.
Genetically Determined Circulating Levels of Cytokines and the Risk of Rheumatoid Arthritis
Authors: Qian Yu , He Zhixing , Zhao Sizheng Steven , Liu Bin , Chen Ying , Sun Xiaohui , Ye Ding , Jiang Xia , Zheng Hui , Wen Chengping , Zheng Houfeng , Mao Yingying 
Published: February 2022

Background: Accumulation of inflammatory leukocytes in articular tissues is the hallmark feature of rheumatoid arthritis (RA). Increasing evidence from observational studies has suggested that several cytokines may be involved in the development of RA. However, traditional observational studies are susceptible to bias from confounding and reverse causation; therefore, the potential causal relationships of individual cytokines with the risk of RA remain elusive.Objective: In this study, we evaluated whether genetically determined circulating levels of cytokines were associated with the risk of RA by performing Mendelian randomization (MR).Methods: We identified single nucleotide polymorphisms (SNPs) associated with circulating levels of cytokines and growth factors from a genome-wide association study (GWAS) including 8,293 participants of Finnish ancestry as instrumental variables (IVs). The association estimates of these IVs with the risk of RA were obtained from a GWAS meta-analysis including 14,361 RA cases and 43,923 controls of European ancestry. We conducted a series of MR analyses to assess the relationship between genetically determined circulating cytokines and the risk of RA, including the random-effects inverse variance-weighted, weighted-median, MR-Egger regression, and MR pleiotropy residual sum and outlier tests. For potential cytokine-RA associations supported by MR evidence, sensitivity analyses were further performed using restricted IV sets of SNPs with colocalization evidence and that excluding pleiotropic SNPs.Results: In the primary MR analysis, there was a suggestive inverse association between genetically determined circulating level of macrophage inflammatory protein-1β (MIP-1b) and the risk of RA [odds ratio (OR): 0.95, 95% confidence interval (CI) = 0.92-0.99, p = 0.016]. The effect estimates were similar in alternative MR analyses. Among SNPs used as IVs for MIP-1b, we found 92 SNPs without documented pleiotropy and three SNPs with evidence of colocalization. The association of MIP-1b with RA from sensitivity analyses using these two sets of restricted IVs remained stable.Conclusion: Our study suggests that genetically determined elevated circulating level of MIP-1b may be associated with a lower risk of RA. Further studies are warranted to determine how MIP-1b and related pathways may contribute to the development of RA.