bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Pig-a conditional knock-out mice model mediated by Vav-iCre: stable GPI-deficient and mild hemolysis
Authors: Chen Yingying , Liu Hui , Zeng Lijie , Li Liyan , Lu Dan , Liu Zhaoyun , Fu Rong 
Published: January 2022

AbstractParoxysmal nocturnal hemoglobinuria is a clonal disease caused by PIG-A mutation of hematopoietic stem cells. At present, there is no suitable PNH animal model for basic research, therefore, it is urgent to establish a stable animal model. We constructed a Pig-a conditional knock-out mice model by ES targeting technique and Vav-iCre. The expressions of GPI and GPI-AP were almost completely absent in CKO homozygote mice, and the proportion of the deficiency remained stable from birth. In CKO heterozygote mice, the proportion of the deficiency of GPI and GPI-AP was partially absent and decreased gradually from birth until it reached a stable level at 3 months after birth and remained there for life. Compared with normal C57BL/6N mice and Flox mice, pancytopenia was found in CKO homozygous mice, and leukopenia and anemia were found in CKO heterozygotes mice. Meanwhile, in CKO mice, the serum LDH, TBIL, IBIL, complement C5b-9 levels were increased, and the concentration of plasma FHb was increased. Hemosiderin granulosa cells can be seen more easily in the spleens of CKO mice. What’s more, CKO mice had stable transcription characteristics. In conclusion, our mouse model has stable GPI-deficient and mild hemolysis, which may be an ideal in vivo experimental model for PNH.
Kinetic modeling and process analysis for photo-production of β-carotene in Dunaliella salina
Authors: Xi Yimei , Zhang Jiali , Kong Fantao , Che Jian , Chi Zhanyou 
Published: January 2022

AbstractDunaliella salina is a green microalga with the great potential to generate natural β-carotene. However, the corresponding mathematical models to guide optimized production of β-carotene in Dunaliella salina (D. salina) are not yet available. In this study, dynamic models were proposed to simulate effects of environmental factors on cell growth and β-carotene production in D. salina using online monitoring system. Moreover, the identification model of the parameter variables was established, and an adaptive particle swarm optimization algorithm based on parameter sensitivity analysis was constructed to solve the premature problem of particle swarm algorithm. The proposed kinetic model is characterized by high accuracy and predictability through experimental verification, which indicates its competence for future process design, control, and optimization. Based on the model established in this study, the optimal environmental factors for both β-carotene production and microalgae growth were identified. The approaches created are potentially useful for microalga Dunaliella salina cultivation and high-value β-carotene production.
Graphical Abstract
Metagenomic community composition and resistome analysis in a full-scale cold climate wastewater treatment plant
Authors: Jankowski Paul , Gan Jaydon , Le Tri , McKennitt Michaela , Garcia Audrey , Yanaç Kadir , Yuan Qiuyan , Uyaguari-Diaz Miguel 
Published: January 2022

AbstractBackgroundWastewater treatment plants are an essential part of maintaining the health and safety of the general public. However, they are also an anthropogenic source of antibiotic resistance genes. In this study, we characterized the resistome, the distribution of classes 1–3 integron-integrase genes (intI1, intI2, and intI3) as mobile genetic element biomarkers, and the bacterial and phage community compositions in the North End Sewage Treatment Plant in Winnipeg, Manitoba. Samples were collected from raw sewage, returned activated sludge, final effluent, and dewatered sludge. A total of 28 bacterial and viral metagenomes were sequenced over two seasons, fall and winter. Integron-integrase genes, the 16S rRNA gene, and the coliform beta-glucuronidase gene were also quantified during this time period.ResultsBacterial classes observed above 1% relative abundance in all treatments were Actinobacteria (39.24% ± 0.25%), Beta-proteobacteria (23.99% ± 0.16%), Gamma-proteobacteria (11.06% ± 0.09%), and Alpha-proteobacteria (9.18 ± 0.04%). Families within the Caudovirales order:Siphoviridae(48.69% ± 0.10%),Podoviridae(23.99% ± 0.07%), andMyoviridae(19.94% ± 0.09%) were the dominant phage observed throughout the NESTP. The most abundant bacterial genera (in terms of average percent relative abundance) in influent, returned activated sludge, final effluent, and sludge, respectively, includesMycobacterium(37.4%, 18.3%, 46.1%, and 7.7%),Acidovorax(8.9%, 10.8%, 5.4%, and 1.3%), andPolaromonas(2.5%, 3.3%, 1.4%, and 0.4%). The most abundant class of antibiotic resistance in bacterial samples was tetracycline resistance (17.86% ± 0.03%) followed by peptide antibiotics (14.24% ± 0.03%), and macrolides (10.63% ± 0.02%). Similarly, the phage samples contained a higher prevalence of macrolide (30.12% ± 0.30%), peptide antibiotic (10.78% ± 0.13%), and tetracycline (8.69% ± 0.11%) resistance. In addition,intI1was the most abundant integron-integrase gene throughout treatment (1.14 × 104gene copies/mL) followed byintI3(4.97 × 103gene copies/mL) whileintI2abundance remained low (6.4 × 101gene copies/mL).ConclusionsWastewater treatment successfully reduced the abundance of bacteria, DNA phage and antibiotic resistance genes although many antibiotic resistance genes remained in effluent and biosolids. The presence of integron-integrase genes throughout treatment and in effluent suggests that antibiotic resistance genes could be actively disseminating resistance between both environmental and pathogenic bacteria.
Examining sex differences in the completeness of Peruvian CRVS data and adult mortality estimates
Authors: Castanheira Helena Cruz , Monteiro da Silva José Henrique Costa 
Published: January 2022

AbstractThe production, compilation, and publication of death registration records is complex and usually involves many institutions. Assessing available data and the evolution of the completeness of the data compiled based on demographic techniques and other available data sources is of great importance for countries and for having timely and disaggregated mortality estimates. In this paper, we assess whether it is reasonable, based on the available data, to assume that there is a sex difference in the completeness of male and female death records in Peru in the last 30 years. In addition, we assess how the gap may have evolved with time by applying two-census death distribution methods on health-related registries and analyzing the information from the Demographic and Health Surveys and civil registries. Our findings suggest that there is no significant sex difference in the completeness of male and female health-related registries and, consequently, the sex gap currently observed in adult mortality estimates might be overestimated.
The endometriosis daily diary: qualitative research to explore the patient experience of endometriosis and inform the development of a patient-reported outcome (PRO) for endometriosis-related pain
Authors: Guan Yanfen , Nguyen Allison M. , Wratten Samantha , Randhawa Sharan , Weaver Jessica , Arbelaez Felipe , Fauconnier Arnaud , Panter Charlotte 
Published: January 2022

AbstractPurposeEndometriosis is a chronic disorder of the female reproductive system characterized by debilitating symptoms, particularly endometriosis-related pain (ERP). Patient-reported outcome (PRO) measures of symptoms and impacts are required to assess disease severity in ERP clinical studies and clinical practice. A content-valid instrument was developed by modifying the Dysmenorrhea Daily Diary (DysDD) to form the Endometriosis Daily Diary (EDD), an electronic PRO administered via handheld device.MethodsQualitative research with US females with ERP was conducted in three stages: (1) Development of an endometriosis conceptual model based on qualitative literature and conduct of concept elicitation (CE) interviews (N = 30). (2) Cognitive debriefing (CD) interviews (N = 30) conducted across two rounds to assess relevance and understanding of the EDD, with modifications between interview rounds. (3) Pilot testing to assess usability/feasibility of administrating the EDD daily on an electronic handheld device (N = 15). Clinical experts provided guidance throughout the study.ResultsThe conceptual model provided a comprehensive summary of endometriosis to inform modifications to the DysDD, forming the EDD. CD results demonstrated that EDD items were relevant for most participants. Instructions, items, response scales, and recall period were well-understood. The resulting daily diary assesses severity of cyclic and non-cyclic pelvic pain, dyspareunia, impact of ERP on functioning and daily life, symptoms associated with ERP, and bowel symptoms. Participants were able to complete the diary daily and found the device easy to use.ConclusionThe EDD demonstrated good content validity in females experiencing ERP. The next step is to perform psychometric validation in an ERP sample.
Do patient-reported outcome measures measure up? A qualitative study to examine perceptions and experiences with heart failure proms among diverse, low-income patients
Authors: Davis Jonathan , Olazo Kristan , Sierra Maribel , Tarver Michelle E. , Caldwell Brittany , Saha Anindita , Lisker Sarah , Lyles Courtney , Sarkar Urmimala 
Published: January 2022

Abstract
Background
The Kansas City Cardiomyopathy Questionnaire (KCCQ) is a Patient-Reported Outcome Measure (PROM) used to evaluate the health status of patients with heart failure (HF) but has predominantly been tested in settings serving predominately white, male, and economically well-resourced populations. We sought to examine the acceptability of the shorter version of the KCCQ (KCCQ-12) among racially and ethnically diverse patients receiving care in an urban, safety-net setting.

Methods
We conducted cognitive interviews with a diverse population of patients with heart failure in a safety net system to assess their perceptions of the KCCQ-12. We conducted a thematic analysis of the qualitative data then mapped themes to the Capability, Opportunity, Motivation Model of Behavior framework.

Results
We interviewed 18 patients with heart failure and found that patients broadly endorsed the concepts of the KCCQ-12 with minor suggestions to improve the instrument’s content and appearance. Although patients accepted the KCCQ-12, we found that the instrument did not adequately measure aspects of health care and quality of life that patients identified as being important components of managing their heart failure. Patient-important factors of heart failure management coalesced into three main themes: social support, health care environment, and mental health.

Conclusions
Patients from this diverse, low-income, majority non-white population experience unique challenges and circumstances that impact their ability to manage disease. In this study, patients were receptive to the KCCQ-12 as a tool but perceived that it did not adequately capture key health components such as mental health and social relationships that deeply impact their ability to manage HF. Further study on the incorporation of social determinants of health into PROMs could make them more useful tools in evaluating and managing HF in diverse, underserved populations.
Venous and arterial cerebral thrombosis: a COVID-19 dual pathology and single possible etiology—a case report
Authors: Roushdy Tamer , Sharaf Nouran K. 
Published: January 2022

Abstract
Background
Corona virus disease of the year 2019 (COVID-19) is still devastating the world for more than 19 months since its declaration as a pandemic by world health organization. Its manifestations does not stand at respiratory system but involves other body systems including central nervous system and its vasculature. In the following case report, cerebral venous and arterial thrombosis is detected in a case just in convalescence from COVID-19 with still detected positive IgM.

Case presentation
A 68-year-old female presenting with disturbed conscious level, bilateral convergent squint, single attack of generalized seizures, left sided dense weakness within a short time from catching COVID-19 and while still in quarantine hospital in recovery phase from infection. Magnetic resonance studies revealed bilateral cortical border zone infarcts as well as left lateral dural sinus and deep venous thrombosis.

Conclusion
Along the forth wave, COVID-19 is still hitting hardly the central nervous system vasculature.
QSAR studies of BBR analogues against coxsackievirus B1
Authors: Obadawo Babatunde Samuel , Asogwa Uchenna , Ali Abdualbaset Ahmed 
Published: January 2022

Abstract
Background
Coxsackievirus group B (CVBs) are common enteroviruses associated with several diseases from etiologically to inflammatory cardiomyopathies and constitute a severe cause of mortality in newborn resulting in severe meningitis, fulminant infection, myocarditis, and encephalitis. While Berberian (BBR) is an effective antivirus and possesses potentials of suppressing CVB replication, Zeng et al. explored a structural modification of BBR by incorporating a substituted primary amine enhance antiviral potency and safety. Based on data set from Zeng et al., we attempted to propose a QSAR model that can predict the bioactivity of unknown compounds as anti-CVB1.

Results
Among many descriptors, four were selected using the Genetic Functional Approximation (GFA). Internal and external validation was carried out on data set using statistical parameters. The QSAR model was seen to meet the minimum requirement with Lack of fit = 0.068744, R2 0.897, Adjusted R2 = 0.8627, cross-validated R2 = 0.76169, R2 predicted = 0.68.

Conclusion
The predictive ability of the model was found to be satisfactory and could be used for designing a similar group of compounds.
Pontic Greek cuisine: the most common foods, ingredients, and dishes presented in cookbooks and folklore literature
Authors: Keramaris Achillefs , Kasapidou Eleni , Mitlianga Paraskevi 
Published: January 2022

Abstract
Introduction
The Pontic Greeks, besides their long and distinguished history, have a special and important culture and identity, elements of which are still preserved and active by their descendants a century after their settlement in Greece. One element of their identity and culture is their basic yet diverse cuisine, which is an important and recognized local cuisine in contemporary Greece. This study aimed to identify the most common foods, ingredients, and dishes found in Greek Pontic Cuisine.

Methods
Six cookbooks, two cooking magazines, four folklore books, and four folklore magazines were reviewed in this study. A considerable amount of data was collected and processed using a text analysis tool.

Results and discussion
The study provides the most frequently encountered dishes, foods, and ingredients that feature in the publications. The most common dishes are soups, including tanomenon sorva (soup with coarse grains, salty strained yogurt, and mint). Among other dishes, siron (a pre-baked filo-based pastry dish), chavitz (a thick corn dish resembling porridge), and foustoron (an omelet with fresh cow butter) are quite common. Common staples are anchovies and greens. In cookbooks and cookery magazines, ingredients include butter, wheat, eggs, tomatoes, milk, bulgur, corn-flour, and cheese. Meanwhile, the study publications are an excellent way of passing down traditional food knowledge intergenerational, as they are largely descended from Pontic Greek progenitors.

Conclusion
After analyzing all the publications, it was declared that dairy products, grains, and vegetables were commonly used in Pontic cuisine. It was concluded that cookbooks are crucial for the preservation of the Greek Pontic culinary tradition.
Collagen-based materials in reproductive medicine and engineered reproductive tissues
Authors: Chen Hanxiao , Xue Luqi , Gong Guidong , Pan Jiezhou , Wang Xiaoling , Zhang Yaoyao , Guo Junling , Qin Lang 
Published: January 2022

AbstractCollagen, the main component of mammal skin, has been traditionally used in leather manufacturing for thousands of years due to its diverse physicochemical properties. Collagen is the most abundant protein in mammals and the main component of the extracellular matrix (ECM). The properties of collagen also make it an ideal building block for the engineering of materials for a range of biomedical applications. Reproductive medicine, especially human fertility preservation strategies and reproductive organ regeneration, has attracted significant attention in recent years as it is key in resolving the growing social concern over aging populations worldwide. Collagen-based biomaterials such as collagen hydrogels, decellularized ECM (dECM), and bioengineering techniques including collagen-based 3D bioprinting have facilitated the engineering of reproductive tissues. This review summarizes the recent progress in applying collagen-based biomaterials in reproductive. Furthermore, we discuss the prospects of collagen-based materials for engineering artificial reproductive tissues, hormone replacement therapy, and reproductive organ reconstruction, aiming to inspire new thoughts and advancements in engineered reproductive tissues research.
Graphical abstract
Polycaprolactone strengthening keratin/bioactive glass composite scaffolds with double cross-linking networks for potential application in bone repair
Authors: Sun Liying , Li Shan , Yang Kaifeng , Wang Junchao , Li Zhengjun , Dan Nianhua 
Published: January 2022

AbstractIn this study, we aimed at constructing polycaprolactone (PCL) reinforced keratin/bioactive glass composite scaffolds with a double cross-linking network structure for potential bone repair application. Thus, the PCL-keratin-BG composite scaffold was prepared by using keratin extracted from wool as main organic component and bioactive glass (BG) as main inorganic component, through both cross-linking systems, such as the thiol-ene click reaction between abundant sulfhydryl groups of keratin and the unsaturated double bond of 3-methacryloxy propyltrimethoxy silane (MPTS), and the amino-epoxy reaction between amino groups of keratin and the epoxy group in (3-glycidoxymethyl) methyldiethoxysilane (GPTMS) molecule, along with introduction of PCL as a reinforcing agent. The success of the thiol-ene reaction was verified by the FTIR and1H-NMR analyses. And the structure of keratin-BG and PCL-keratin-BG composite scaffolds were studied and compared by the FTIR and XRD characterization, which indicated the successful preparation of the PCL-keratin-BG composite scaffold. In addition, the SEM observation, and contact angle and water absorption rate measurements demonstrated that the PCL-keratin-BG composite scaffold has interconnected porous structure, appropriate pore size and good hydrophilicity, which is helpful to cell adhesion, differentiation and proliferation. Importantly, compression experiments showed that, when compared with the keratin-BG composite scaffold, the PCL-keratin-BG composite scaffold increased greatly from 0.91 ± 0.06 MPa and 7.25 ± 1.7 MPa to 1.58 ± 0.21 MPa and 14.14 ± 1.95 MPa, respectively, which suggesting the strong reinforcement of polycaprolactone. In addition, the biomineralization experiment and MTT assay indicated that the PCL-keratin-BG scaffold has good mineralization ability and no-cytotoxicity, which can promote cell adhesion, proliferation and growth. Therefore, the results suggested that the PCL-keratin-BG composite scaffold has the potential as a candidate for application in bone regeneration field.Graphical Abstract
Gold recovery from waste printed circuit boards of mobile phones by using microwave pyrolysis and hydrometallurgical methods
Authors: Huang Yu-Fong , Chou Szu-Ling , Lo Shang-Lien 
Published: January 2022

AbstractRecycling of waste printed circuit boards (PCBs) has attracted increasing attention because of its high annually produced amount and high content of gold. In this study, gold recovery from waste PCBs was carried out by using the processes including microwave pyrolysis, acid leaching, solvent extraction and oxidative precipitation. The leaching efficiency of copper was approximately 95% when using a lixiviant composed of sulfuric acid and hydrogen peroxide, and the leaching efficiencies of gold were approximately 59, 95 and 95% by using thiourea, thiosulfate and aqua regia, respectively. The gold ions contained in the leachate previously produced by the leaching processes were not satisfactorily extracted by using organic solvents including di-(2-ethylhexyl)phosphoric acid, tributyl phosphate, dibutyl carbitol and trioctylamine, so the leachate was decided to bypass solvent extraction and directly apply to the oxidative precipitation process. By using the oxidants of hydrogen peroxide and perchloric acid, the precipitation efficiencies of gold were approximately 95 and 99%, and the final recovery rates were approximately 90 and 93%, respectively. The high recovery rates of gold can be attributable to the use of microwave pyrolysis that prevents the loss of gold caused by shredding and grinding processes. In addition, perchloric acid can provide higher selectivity for gold recovery than hydrogen peroxide. The maximum processing capacity of microwave pyrolysis of waste PCBs would be approximately 1.23 kg. The gold recovered from 1 t of waste PCBs can be sold for approximately USD 10,000, and thus the return on investment can be as high as approximately 1400%.
Toward the formulation of a proposed frame for the formal and structural specifications of the modern parametric digital architecture
Authors: Tantawy Amal R. 
Published: January 2022

Abstract
Background
This research paper contributes to presenting a proposed framework for the formal and structural specifications of parametric digital architecture in order to increase knowledge and know-how in this field closely to specialists and those interested in it through the research methodology, which is divided first into the theoretical background to include a presentation of the definition of digital architecture and the most important pioneers of digital architecture, digital building materials and construction mechanisms parametric digital and what are the most important computer software used in parametric digital architecture in terms of design and construction implementation and identifying the most important digital specifications and characteristics that were mentioned in previous studies in this field and what are the unconventional digital esthetic values, then comes the role of the applied study in formulating a comprehensive matrix of parametric design modern and its impact on the development of traditional architectural models in digital architecture.

Results
The research study found that both (unrealistic shape) and (typical interaction) ranked first with the highest percentage reaching 100% in all study cases. The ratio of the volume of each to the total volume of all elements of morphological characteristics and non-traditional esthetic values was 8%, the most important characteristic of buildings in parametric digital architecture, in most of them, is the lack of realism to a very significant degree, and we always find a clear increase in the interaction, vitality and dynamism of buildings with the surrounding environment.

Conclusions
The most important characteristic of buildings in parametric digital architecture, in most of them, is the lack of realism to a very significant degree, and we always find a clear increase in the interaction, vitality and dynamism of buildings with the surrounding environment. While we conclude that the least characteristic of the parametric digital buildings is the simulation of nature or the tendency to everything that is traditional, as well it is often inclined to everything strange and unfamiliar.