bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dielectricity of a molecularly crowded solution accelerates NTP misincorporation during RNA-dependent RNA polymerization by T7 RNA polymerase
Authors: Takahashi Shuntaro , Matsumoto Saki , Chilka Pallavi , Ghosh Saptarshi , Okura Hiromichi , Sugimoto Naoki 
Published: January 2022

AbstractIn biological systems, the synthesis of nucleic acids, such as DNA and RNA, is catalyzed by enzymes in various aqueous solutions. However, substrate specificity is derived from the chemical properties of the residues, which implies that perturbations of the solution environment may cause changes in the fidelity of the reaction. Here, we investigated non-promoter-based synthesis of RNA using T7 RNA polymerase (T7 RNAP) directed by an RNA template in the presence of polyethylene glycol (PEG) of various molecular weights, which can affect polymerization fidelity by altering the solution properties. We found that the mismatch extensions of RNA propagated downstream polymerization. Furthermore, PEG promoted the polymerization of non-complementary ribonucleoside triphosphates, mainly due to the decrease in the dielectric constant of the solution. These results indicate that the mismatch extension of RNA-dependent RNA polymerization by T7 RNAP is driven by the stacking interaction of bases of the primer end and the incorporated nucleotide triphosphates (NTP) rather than base pairing between them. Thus, proteinaceous RNA polymerase may display different substrate specificity with changes in dielectricity caused by molecular crowding conditions, which can result in increased genetic diversity without proteinaceous modification.
Influence of methane seepage on isotopic signatures in living deep-sea benthic foraminifera, 79° N
Authors: Melaniuk Katarzyna , Sztybor Kamila , Treude Tina , Sommer Stefan , Rasmussen Tine L. 
Published: January 2022

AbstractFossil benthic foraminifera are used to trace past methane release linked to climate change. However, it is still debated whether isotopic signatures of living foraminifera from methane-charged sediments reflect incorporation of methane-derived carbon. A deeper understanding of isotopic signatures of living benthic foraminifera from methane-rich environments will help to improve reconstructions of methane release in the past and better predict the impact of future climate warming on methane seepage. Here, we present isotopic signatures (δ13C and δ18O) of foraminiferal calcite together with biogeochemical data from Arctic seep environments from c. 1200 m water depth, Vestnesa Ridge, 79° N, Fram Strait. Lowest δ13C values were recorded in shells of Melonis barleeanus, − 5.2‰ in live specimens and − 6.5‰ in empty shells, from sediments dominated by aerobic (MOx) and anaerobic oxidation of methane (AOM), respectively. Our data indicate that foraminifera actively incorporate methane-derived carbon when living in sediments with moderate seepage activity, while in sediments with high seepage activity the poisonous sulfidic environment leads to death of the foraminifera and an overgrowth of their empty shells by methane-derived authigenic carbonates. We propose that the incorporation of methane-derived carbon in living foraminifera occurs via feeding on methanotrophic bacteria and/or incorporation of ambient dissolved inorganic carbon.
Empirical study of employee loyalty and satisfaction in the mining industry using structural equation modeling
Authors: Chen Shoukun , Xu Kaili , Yao Xiwen 
Published: January 2022

AbstractMining is a high-risk industry and a crucial economic driver that has a crucial role in the economies of countries worldwide. The implications of the labor market on the sustainability of the mining industry have increased the importance of sustainable human resource management at the strategic level of mining and safety management. In this article, from the perspective of management research in an energy production enterprise, we investigated the relationship between employee loyalty and employee satisfaction through a survey that targets employee loyalty, work quality, and job satisfaction and the relationship between enterprise image and switching costs. Based on service profit chain theory, we established a research model for mining employee loyalty, and 500 miners in a typical extreme mining environment in China were surveyed. The study hypotheses were tested using a structural equation model and an employee loyalty model, followed by empirical testing of the models. Employee loyalty was significantly associated with enterprise image and employee satisfaction, work quality indirectly affected loyalty through satisfaction, and the impact of switching costs on employee loyalty was not significant. We provide strong empirical evidence to help enterprises improve sustainable human resource management and regulatory policies, with important implications for safety production. Our study also provides a useful reference for further studies of sustainable human resource management in mining.
The merging process of chromospheric fibrils into a filament
Authors: Fang Y. , Zhang J. , Song Z. P. , Hou Y. J. , Li T. 
Published: January 2022

Context. Although solar filaments have been intensively studied, detailed observations that show an entire process of filament maintenance are rare.
Aims. The aim of this paper is to study the whole process of the material supply and the magnetic flux injection from chromospheric fibrils to a nearby filament.
Methods. Based on multiwavelength observations from the New Vacuum Solar Telescope and the Solar Dynamics Observatory (SDO), we tracked the evolution of the chromospheric fibrils involved in the process of filament maintenance and estimated the relevant kinetic parameters. The possible reconnection process was further analyzed in detail by using the SDO magnetic field and extreme ultraviolet observations.
Results. In the southeast of the filament, two sets of chromospheric fibrils approach and interact with each other, accompanied by weak brightening at the interacting region. Subsequently, a long fibril is formed, keeps moving toward the filament, and finally merges into it. The mergence results in a disturbance in the filament, for example, some of the original filament fibrils move northward. Ten minutes later, a similar process occurs again. By checking the photospheric magnetograms, we find that the two sets of chromospheric fibrils are rooted in a pair of opposite-polarity magnetic patches, and magnetic cancellation takes place between them. We propose that magnetic reconnection could occur between chromospheric fibrils and that it plays an important role in the formation of the new longer fibrils.
Conclusions. Magnetic reconnections between chromospheric fibrils produce new fibrils, which then merge into a nearby filament. Such observations imply that filament material and magnetic flux can be supplied from surrounding chromospheric fibrils.
A Comparative Analysis of Transcranial and Endoscopic Repair for Traumatic CSF Rhinorrhea
Authors: Vasu Srivatsan Thirumalai , Krishnamoorthy Venkidesh , Karunakaran Sudish , Pillay Haroon M. 
Published: January 2022

Abstract
Background Surgical treatment of anterior cranial base traumatic cerebrospinal fluid (CSF) rhinorrhea is challenging and is fraught with complications. Whether a person should be offered open craniotomy or endoscopic endonasal repair is a dilemma faced by most surgeons. This study is one of the few to directly compare the two forms of management.
Methods Data were collected from two groups of 15 patients each who underwent transcranial CSF leak repair and endoscopic endonasal CSF leak repair in a tertiary care hospital over a 3-year period. Information including demographics, recurrence rates, complications, and hospital and intensive care unit (ICU) stay was recorded and analyzed. Outcome was assessed up to 6 months.
Results Recurrence was seen in 9/30 patients, 6 in the transcranial group and 3 in the endoscopic group. Hospital stay was longer than 1 week in all the transcranially operated patients and only in 73% of the endoscopically operated patients (p = 0.439) although ICU stay was reduced in the endoscopic group (p = 0.066). Complications were more common with transcranial repair (seven of eight patients who underwent transcranial repair, p = 0.035) with anosmia being the most common (33.3%, p = 0.042).
Conclusion The transcranial open repair is a reasonable choice especially for leaks that occur through the frontal sinus and extend backward into the frontoethmoidal region. However, this approach has the drawbacks of greater number of complications, higher recurrence rate, and longer ICU and overall hospital stay. The endoscopic endonasal repair enjoys a lower morbidity profile although it may not be an adequate treatment for leaks that are placed far laterally in the frontal sinus.
Pseudomonas aeruginosa Pneumonia: Evolution of Antimicrobial Resistance and Implications for Therapy
Authors: Lynch Joseph P. , Zhanel George G. 
Published: January 2022

AbstractPseudomonas aeruginosa (PA), a non–lactose-fermenting gram-negative bacillus, is a common cause of nosocomial infections in critically ill or debilitated patients, particularly ventilator-associated pneumonia (VAP), and infections of urinary tract, intra-abdominal, wounds, skin/soft tissue, and bloodstream. PA rarely affects healthy individuals, but may cause serious infections in patients with chronic structural lung disease, comorbidities, advanced age, impaired immune defenses, or with medical devices (e.g., urinary or intravascular catheters, foreign bodies). Treatment of pseudomonal infections is difficult, as PA is intrinsically resistant to multiple antimicrobials, and may acquire new resistance determinants even while on antimicrobial therapy. Mortality associated with pseudomonal VAP or bacteremias is high (> 35%) and optimal therapy is controversial. Over the past three decades, antimicrobial resistance (AMR) among PA has escalated globally, via dissemination of several international multidrug resistant “epidemic” clones. We discuss the importance of PA as a cause of pneumonia including health care–associated pneumonia, hospital-acquired pneumonia, VAP, the emergence of AMR to this pathogen, and approaches to therapy (both empirical and definitive).
Configuration spaces of hinged mechanisms, and their projections
Authors: Kovalev M. D. 
Published: January 2022

Abstract
Our subject is the geometry of planar hinged mechanisms. The article contains a formalization of basic concepts of the theory of hinged-lever constructions, as well as some information from real algebraic geometry needed for their study. We consider mechanisms with variable number of degrees of freedom and mechanisms that have more than one degree of freedom but each hinge of which moves with one degree of freedom. For the last type we find the dimension of the configuration space. We give a number of examples of mechanisms with unusual geometric properties and formulate open questions.
Bibliography: 17 titles.
Broadcasting of amplitude- and frequency-modulated c-di-GMP signals facilitates cooperative surface commitment in bacterial lineages
Authors: Lee Calvin K. , Schmidt William C. , Webster Shanice S. , Chen Jonathan W. , O’Toole George A. , Wong Gerard C. L. 
Published: January 2022

SignificanceIt is well known that c-di-GMP concentration rises in surface-sensing bacteria and functions as a “molecular switch” for biofilm formation. Here, we provide an important recasting of this picture: Intracellular c-di-GMP signals do not just increase in surface-sensing bacteria; such signals are cooperatively broadcast across multiple generations of cells in a lineage with oscillations that undergo both amplitude and frequency modulation, which are controlled by the coupling between pili appendages and c-di-GMP synthesis machinery. The right “tuning” of these signals in terms of frequency and amplitude correlates ultimately to surface commitment. Amplitude and frequency modulation of c-di-GMP signals allows encoding of more complex instructions. Thus, our work provides a more nuanced understanding of how c-di-GMP signaling drives surface commitment.
The context of the ribosome binding site in mRNAs defines specificity of action of kasugamycin, an inhibitor of translation initiation
Authors: Zhang Yan , Aleksashin Nikolay A. , Klepacki Dorota , Anderson Caleb , Vázquez-Laslop Nora , Gross Carol A. , Mankin Alexander S. 
Published: January 2022

Significance
Several antibiotics targeting the large ribosomal subunit interfere with translation in a context-specific manner, preventing ribosomes from polymerizing specific amino acid sequences. Here, we reveal kasugamycin as a small ribosomal subunit-targeting antibiotic whose action depends on the sequence context of the untranslated messenger RNA (mRNA) segments. We show that kasugamycin-induced ribosomal arrest at the start codons of the genes and the resulting inhibition of gene expression depend on the nature of the mRNA nucleotide immediately preceding the start codon and on the proximity of the stop codon of the upstream cistron. Our findings underlie the importance of mRNA context for the action of protein synthesis inhibitors and might help to guide the development of better antibiotics.
Liquid polarity, positional contrast, and diachronic change
Authors: Sen Ranjan , Zair Nicholas 
Published: January 2022

AbstractApparently disparate sound changes in Latin, involving both vowels and consonants but sensitive to /r/, can be explained by reconstructing a positional clear/dark contrast in /r/, motivated by the seldom-mentioned “liquid polarity” effect. Examining these diachronic processes together allows us to see a larger picture, providing evidence for the reconstruction of successive past synchronic states. Latin /r/ mirrored the behaviour of Latin /l/ up to the first century BC: /l/ was dark and /r/ was clear in codas, and /r/ was dark and /l/ was underspecified for tongue body position in onsets. Darkness in /r/ was partly implemented through the selection of r-type: dark onset approximant and clear coda tap. Later, coda /r/ became an approximant like onset /r/, and subsequently both became trills, resulting in the erosion of the positional contrast and the liquid polarity effect.
On the conditional marker “zhě”(者)
Authors: Li Fanxi 
Published: January 2022

AbstractThis paper aims to explain the development of “zhě” serving as a conditional marker mainly in Late Archaic Chinese and to show that the conditional marker “zhě” derives from the nominalizer “zhě”. The grammaticalization of “zhě” is at the level of pragmatic inference, which shows an interaction of nominalization, topicality, and conditionality. Nominalization directly makes the original clause lose its ability to confirm the factuality of the event, and the nominalized construction then is endowed with a generic meaning, which is in some way related to conditionals. Specifically, the contexts triggering this grammaticalization can be summarized as follows: Firstly, the construction “VP/clause + zhě” always occupies the topic position; secondly, VPs/clauses in that construction always represent a kind of non-factual event, which means that the semantic feature of the VPs/clauses is non-factual or unreal. Furthermore, there are two clues, namely modal auxiliary and negator, which can help identify the non-factual event.
Language policy and planning for English-medium instruction in higher education
Authors: Ou Amy Wanyu , Hult Francis M. , Gu Michelle Mingyue 
Published: January 2022

AbstractThis article presents English-medium instruction (EMI) in higher education (HE) from a language policy and planning (LPP) perspective. Based on a review of EMI policy research in diverse higher education contexts, we address several key contemporary policy tensions in EMI such as English native-speakerism, English monolingualism, and language education with attention to corpus, status and acquisition planning as well as the role of individual policy actors. In light of this review, we argue for an orientation to policymaking for EMI-HE institutions that acknowledges the value of individuals as policy arbiters and aligns institutional goals with “on the ground” needs and practices. We also point to areas of future research that would benefit all EMI stakeholders, in particular critical engagement with the nature of language competence in EMI settings and the enhanced development of evidence-based EMI learning outcomes. Finally, we propose an ecological framework for EMI-HE policy development that could be employed as a heuristic to guide universities in designing concrete EMI policies for their local contexts. As an extension, we also offer an inventory of reflective questions to guide key university stakeholders in effectively engaging in EMI policy processes.
The effects of Ti3C2 MXene additive on lithiation induced stress in silicon/graphite-based electrodes for lithium ion batteries
Authors: Wang Zhuo , Liu Jishan , Chen Kongyao , Wu Xueling , Mi Liwei , Mao Weiguo 
Published: January 2022

Abstract
The lithiation induced stress in multilayer silicon/graphite-based electrodes for lithium ion batteries is evaluated analytically. Firstly, the silicon/graphite-based composite active layer is reinforced by Ti3C2 MXene, and the effects of Ti3C2 MXene additive on elastic modulus of the active layer are discussed based on the Halpin–Tsai empirical equation. Secondly, biaxial stresses in both bilayer and symmetric trilayer silicon/graphite-based electrodes are estimated by a mechano-electrochemical model, which is derived by imitating thermal expansion stress in layered structural components. And the effects of Ti3C2 MXene’s parameters, including additive amount, number of layers and length–width ratio, on the stress in multilayer silicon/graphite-based electrodes are also be discussed in detail. Finally, based on the numerical analysis, some design strategies for Ti3C2 MXene reinforced composite active layer are proposed from mechanical views.
Effective field theory analysis of 3He–α scattering data
Authors: Poudel Maheshwor , Phillips Daniel R 
Published: January 2022

Abstract
We treat low-energy 3He–α elastic scattering in an effective field theory (EFT) that exploits the separation of scales in this reaction. We compute the amplitude up to next-to-next-to-leading order, developing a hierarchy of the effective-range parameters (ERPs) that contribute at various orders. We use the resulting formalism to analyse data for recent measurements at center-of-mass energies of 0.38–3.12 MeV using the scattering of nuclei in inverse kinematics (SONIK) gas target at TRIUMF as well as older data in this energy regime. We employ a likelihood function that incorporates the theoretical uncertainty due to truncation of the EFT and use Markov chain Monte Carlo sampling to obtain the resulting posterior probability distribution. We find that the inclusion of a small amount of data on the analysing power A

y
is crucial to determine the sign of the p-wave splitting in such an analysis. The combination of A

y
and SONIK data constrains all ERPs up to O(p
4) in both s- and p-waves quite well. The asymptotic normalisation coefficients and s-wave scattering length are consistent with a recent EFT analysis of the capture reaction 3He(α, γ)7Be.
XPS binding energy shifts as a function of bond distances: a case study of CO
Authors: Bagus Paul S , Sousa Carmen , Illas Francesc 
Published: January 2022

Abstract
The O(1s) and C(1s) XPS core-level binding energies. BEs, have been studied as a function of the C–O internuclear distance for a large range of distances. The BE(r) for both BEs show considerable variation over the distances studied which is, however, different for the O(1s) and C(1s) BEs. The origin of the dependence on C–O distance is established and shown to involve more than the electric field generated because of the charge separation within CO being C+q
and O−q
. Furthermore, the BE(r) is shown to be different for Hartree–Fock and correlated wavefunctions indicating that the BE(r) can provide evidence of how electron correlation modifies the valence charge distribution. The difference between the O(1s) and C(1s) BEs is examined and it is proposed that this difference can be used as a measure of the accuracy of theoretically predicted BEs. It is believed that the features found for CO may be representative for the BE variations with geometry for other systems; an effect that has been mostly overlooked.
Illustrating dimensionless scaling with Hooke’s law
Authors: Bissell J J , Ali A , Postle B J 
Published: January 2022

Abstract
Dimensionless variables and scalings are powerful tools for emphasising the universality of natural laws, but their application is typically restricted to topics in advanced physics. Here we describe how dimensionless scaling can be illustrated in an introductory physics context by considering a dimensionless form for Hooke’s law. As a simple empirical study, we verify this form for Hooke’s law using data from foundation-year degree (high-school level) experiments on springs in series and parallel.
Flexural properties of lightweight carbon fiber/epoxy resin composite sandwiches with different fiber directions
Authors: Liu J A , Dong Z Q , Zhu X Y , Sun W B , Huang Z Q 
Published: January 2022

Abstract
In this study, structurally efficient carbon fiber reinforced plastic (CFRP) sandwiches were developed via introducing pumice/Mg composite foams as new core material. The effects of the fiber direction (0°, 45°, 90°) on the mechanical properties of CFRP laminates and composite sandwiches were studied. Compared with 45°-CFRP and 90°-CFRP laminates, 0°-CFRP laminate exhibits outstanding flexural properties due to different failure modes. Correspondingly, the 0°-CFRP/PMSF composite sandwiches exhibit higher flexural strength than 45°-CFRP/PMSF and 90°-CFRP/PMSF composite sandwiches. The as-prepared composite sandwiches are lightweight and have higher specific strength than some traditional sandwiches. The different flexural behaviors of three types of sandwiches were observed to explain the failure mechanisms.
Induction heating ferromagnetic particles embedded PDMS mold for microstructure embossing
Authors: Hsu Ming-Huai , Tsai Yao-Yang , Yang Sen-Yeu 
Published: January 2022

Abstract
Polydimethylsiloxane (PDMS) is an excellent soft mold material with the advantages of precise replication, easy demolding, and low production cost. However, the strength and hardness of PDMS are relatively low, and PDMS cannot be directly inductively heated. In this study, PDMS is embedded with ferromagnetic powders to increase its hardness and make it heatable. Direct induction heating of the PDMS mold can raise its inherent temperature, increase the heating efficiency by 100% compared with pure PDMS, and improve the shortcomings of uneven surface temperature distribution from high thermal resistance. Furthermore, adding the ferromagnetic metal powder to PDMS can improve its conductivity and make the mold a high-low surface temperature gap as low as 1.6 °C. Adding nickel powder to the PDMS mold makes the hardness 2.29 times higher than that of pure PDMS and can withstand a pressure of 7 kg cm−2, which is very conducive to hot embossing. This study used a self-designed five-sided cladding iron block base and a PDMS mold with ferromagnetic metal powder for hot embossing. This heating apparatus can quickly raise the PDMS surface temperature and emboss deep V-groove microstructures on the polycarbonate (PC) film; the replication performance can reach more than 97%.