bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alternanthera Brasiliana: an Organic Growth Promoter in Poultry
Authors: Ekuma Emmanuel Toochukwu , Oko Augustine Okpani 
Published: May 2022

Abstract
The possible application of aqueous and ethanol leaf-extracts of Alternanthera brasiliana as poultry growth promoters were investigated. Standard methods were used to study the vitamin, mineral and phytochemical contents of the leaves. Seventy-two broilers were grouped into nine of eight birds per treatment. Carcass yield, meat qualities and blood components evaluations were done using standard methods. Results revealed the presence of water-soluble vitamins (B1, B2, B3, B6, B12, C), fat soluble vitamins (A, E, D), minerals and phytochemicals necessary for improving broiler performance. The carcass yield and meat qualities of the test broilers were better than the negative control group. Also, the blood proteins and hematological conditions of the chickens were improved by the extracts better than the negative control group. Communality studies showed that all the blood parameters evaluated were affected by the extracts administered to the broilers. It can therefore, be inferred from the results that leaf-extracts of A. brasiliana could replace chemical growth promoters in poultry production, with the aqueous extracts as a preferred choice, having produced better effects than the ethanol counterpart.
Knockdown of GOLM1 inhibits growth of laryngeal cancer cells via the Wnt/β-catenin pathway
Authors: wang Jiashuo , Wang Sai , Ding Xiaoxu , Hu Yue , Yan Aihui 
Published: May 2022

Abstract
Background Laryngeal squamous cell carcinoma(LSCC) is a common type of malignant tumors of the head and neck. At present, the early biological markers of laryngeal cancer are still the focus of attention by otolaryngologists.Golgi membrane protein 1 (GOLM1) is a transmembrane glycoprotein present in the cis-Golgi complex.In recent years, successive findings have revealed the carcinogenic effects of GOLM1.However, the biological impact of GOLM1 on LSCC has not been clarified.Results In this study, GOLM1 expression was found to be significantly correlated with the progression and prognosis of LSCC. By silencing GOLM1, the Wnt/β-catenin pathway was downregulated, thereby inhibiting cell proliferation, migration, and invasion, as well as the progress of EMT, and promoting TU686 cell apoptosis. Conclusions Our study revealed the influence of GOLM1 through the Wnt/β-catenin pathway on the biological behavior of LSCC. GOLM1 is thus a potential target for the diagnosis and treatment of LSCC.
Cytotoxicity and anti-biofilm activities of biogenic cadmium nanoparticles and cadmium nitrate: a preliminary study
Authors: Adeli-Sardou Mahboubeh , Shakibaie Mojtaba , Forootanfar Hamid , Jabari-Morouei Fereshteh , Riahi-Madvar Soudabe , Ghafari-Shahrbabaki Sima-Sadat , Mehrabani Mitra 
Published: May 2022

Abstract
Cadmium nanoparticles (Cd NPs) were prepared using Artemisia persica extract and microwave irradiation in this research. Cd NPs and Cd (NO3)2 were evaluated for their cytotoxicity, antibacterial, anti-biofilm, and biocompatibility. It was found that Cd NPs (11.2–18.6 nm) had a lower cytotoxic effect than Cd (NO3)2 against both HepG2 and HUVEC cells. The antibacterial activity of Cd NPs against clinically isolated Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa was above 2560 μg mL-1. The Cd NPs (640 μg mL-1) decreased the biofilm formation by S. aureus, P. mirabilis, and P. aeruginosa to 24.6%, 31.6%, and 26.4%, respectively. Adding Cd NPs (100 μg/disc) to commercial antibiotic discs increased the antibacterial activity of vancomycin, gentamicin, tetracycline, streptomycin, meropenem, and kanamycin against Methicillin-resistant S. aureus, significantly. The Cd NPs (640 μg mL-1) exhibited low hemolysis activity. More research should be conducted to understand the related cytotoxicity and antibacterial mechanisms of Cd NPs.
Intentional Travel Groups and Social Networks during the COVID-19 Pandemic
Authors: Zhou Mingzhi , Lei Shuyu , Wu Jiangyu , Ma Hanxi , Levinson David , Zhou Jiangping 
Published: May 2022

Abstract
Although face-to-face social contacts decreased during the COVID-19 pandemic, some people remain physically traveling and meeting as a group to gain benefits like sustaining intimacy and increasing productivity. Using multiday continuous smartcard data, we identify intentional group travel patterns in Hong Kong Metro system. Those patterns serve as our proxies for physical (visible) interactions and social (invisible) contact networks among people who intentionally travel as a group (ITGs). We measure the spatial centrality of ITGs and persistent group riders (PGRs), a subset of ITGs remaining active amid the pandemic, to infer different locales' social interactions in the city. By examining the social network formed by ITGs across time, we found that its size and interconnections varied during the pandemic and PGRs might be the most influential vertices in maintaining the networks’ topological properties. The findings could facilitate transit-usage-and-virus-spread modelling and the formulation of more effective pandemic countermeasures in transit-reliant cities.
Protective effect of isoflurane preconditioning on neurological function in rats with HIE
Authors: Wang Yi-Bo , Chen Ting-Bao , Huang-Fu Li-Ren , Zhou Hong-Su , Xiao Qiu-Xia , Fang Xu , Xiong Liu-Lin , Wang Ting-Hua 
Published: May 2022

Abstract
Objective
Hypoxic ischemic encephalopathy (HIE) is an important cause of neonatal death and disability, which can lead to long-term neurological and motor dysfunction. At present, the treatment mainly focuses on the symptomatic treatment in the acute phase and rehabilitation after injury, but the effect is not satisfactory, and more effective methods are urgently needed. Due to the widespread use of inhalation anesthetics in surgery, it has been found that isoflurane (ISO) preconditioning may have a positive effect on neuroprotection. The aim of this study was to investigate the effects of ISO preconditioning on neurological function and behavior in HIE rats.
Methods
Neonatal rats were used to create hypoxic-ischemic (HI) model by ligating the right common carotid artery at 7 days and then in a hypoxia chamber. The effects of anesthetic drugs on neurological function after one hour of ISO preconditioning were assessed after modeling by Morris water maze(MWM), Y-maze, and rotarod tests at one month. Thereafter, samples were taken at 1 and 42 days for Nissl staining and Hematoxylin-Eosin (HE) staining to observe neuronal number and histomorphology changes. The relationship of behaviour and morphology tests were measured.
Results
Ischemia and hypoxia could induce neuronal injury, neurological dysfunction and severe long-term motor function injury. Histological staining and behavioural evaluations were performed to elucidate the pathological changes and neurobehavioural variation after ISO treatment. We found ISO administration significantly reduced the infarct volume of brain tissues and improved the autonomous activities of neonatal rats, ISO preconditioning significantly reduced neuronal apoptosis induced by HI, reduced cerebral infarction volume, improved histopathological damage of nerve cells, improved long-term cognitive function, and attenuated HI-induced Nissl total cells to reduce and reduce long-term spatial memory impairment caused by hypoxia-ischemia during the maturation of injured brain.
Conclusion
ISO preconditioning can protect the brain injury and promote the recovery of neurological function in neonatal rats with HIE, which may be through inhibiting the neuronal cell death in the cortex and hippocampus after HI.
Effects of Tibetan Plateau summer monsoon on glacier change over the Pamir Plateau during 1980-2012
Authors: Zhao Yong , Ren Guoqiang , Yao Junqiang , Meng Lixia 
Published: May 2022

Abstract
The glacier over the Pamir Plateau had received a slight expansion during 1980-2012, which was different from ones over the most of High Asia. Our study revealed the possible influencing mechanism of the Tibetan Plateau summer monsoon on glacier change over the Pamir Plateau. The Tibetan Plateau summer monsoon could both modulate the T2m and precipitation well related the glacier change over the Pamir Plateau via influencing the middle to upper tropospheric temperatures. One possible physical mechanism was identified. The strong Tibetan Plateau monsoon could result in the cooling of middle to upper tropospheric temperatures over central Asia. On the one hand, the cooling of middle to upper tropospheric temperatures caused the decline of 0℃ level height, which slowed down the warming amplitude of T2m over the Pamir Plateau. On the other hand the cooling of middle to upper tropospheric temperatures could strengthened the occurrence of anomalous cyclone over central Asia and the northward transport of water vapor from the Arabian Sea, which were well related to summer precipitation over the Pamir Plateau. Above both suggested that the strong summer Tibetan Plateau monsoon was contributed the expansion of glacier over the Pamir Plateau.
What is the effect of EU’s fuel-tax cuts on Russia’s oil income?
Authors: Gars Johan , Spiro Daniel , Wachtmeister Henrik 
Published: May 2022

Abstract
Following the oil-price surge in the wake of Russia’s invasion of Ukraine, many countries in the EU are cutting taxes on petrol and diesel. Using standard theory and empirical estimates, we assess how such tax cuts influence the oil income in Russia. We find that a tax cut of 20 euro cents per liter increase Russia’s oil profits by around 11 million Euros per day in the short run and long run. This is equivalent to 4100 million Euros in a year, 0.3% of Russia’s GDP or 7% of its military spending. We show that a cash transfer to EU citizens, with an equivalent fiscal burden as the tax cut, reduces these side effects to a fraction.
CD4 and FOXP3 as predictive markers for the recurrence of T3/T4a stage II colorectal cancer: applying a novel discrete Bayes decision rule
Authors: Nakagami Yuki , Hazama Shoichi , Suzuki Nobuaki , Yoshida Shin , Tomochika Shinobu , Matsui Hiroto , Shindo Yoshitaro , Tokumitsu Yukio , Matsukuma Satoshi , Watanabe Yusaku , Iida Michihisa , Tsunedomi Ryouichi , Takeda Shigeru , Fujita Tomonobu , Kawakami Yutaka , Ogihara Hiroyuki , Hamamoto Yoshihiko , Ioka Tatsuya , Tanabe Tsuyoshi , Ueno Tomio , Nagano Hiroaki 
Published: May 2022

Abstract
Background
We recently reported the relapse-free survival (RFS) significance of the combination of CD4+ and forkhead box P3+ (FOXP3) T-cell densities identified by immunohistochemistry in patients with stage I, II, and III colorectal cancer (CRC) who underwent curative resections. This study was designed to determine the optimal combination of markers that predict recurrence in patients with T factors of T3/T4a stage II CRC by applying a novel Bayes decision rule.
Methods
One hundred thirty-seven cancer tissue specimens from T3/T4a stage II patients with 12 clinicopathologic and immune factors were analysed as predictive candidates for recurrence.
Results
Our study showed that the combination of low CD4+ and low FOXP3 T-cell densities resulted in extremely poor RFS prognoses.
Conclusions
Adjuvant chemotherapy may be considered for patients with a combination of low CD4+ and low FOXP3+ T-cell densities. The discovery of this new prognostic indicator is important for the appropriate management of patients undergoing curative resection for T3/T4a stage II CRC.
Retentive strength of CAD/CAM-fabricated all-ceramic crowns luted on titanium implant abutments using different ceramic materials and luting agents.
Authors: Bjelopavlovic Monika , Weyhrauch Michael , Scheller Herbert , Wentaschek Stefan , Lehmann Karl Martin 
Published: May 2022

Abstract
Objectives.
This study aimed to determine the retentive strength of monolithic hybrid-all-ceramic crowns luted on titanium implant abutments.
Material and Methods.
In total, 450 crowns (75 each of Mark II, Empress CAD, e.max CAD, Suprinity, Enamic, Celtra Duo) were milled using a CAD/CAM system. The crowns were cemented onto sandblasted titanium implant abutments using five luting agents (Multilink Implant, Variolink II, RelyX Unicem, Fujicem, and Panavia 2.0). After thermocycling, the crowns were removed using a universal testing machine. The location of luting-agent residue on the abutment and inner crown surfaces was evaluated. Analyses of variance (ANOVA) with the Bonferroni correction were performed to evaluate differences of retentive strength depending on the crown material and the kind of the luting agent.
Results.
The retentive strength for the different ceramic materials were Vita Mark II: 652N-759N (SD:134N-146N), Empress CAD: 681N-822N (SD: 89N-146N), e.max CAD: 784N-1044N (SD: 109N-176N), Vita Enamic: 716N-1177N (SD: 132N-220N), Vita Suprinity: 867N-1488N (SD: 202N-278N) and Celtra Duo 772N-1335N (SD:151N-229N). After the removal trials, the visual documentation showed different adhesive residue location depending on the ceramic materials. Furthermore, the pull-off force was dependent on the choice of adhesives
Conclusions.
The ceramic material used seems to influence the retentive strength and the use of certain luting agents result for some ceramic materials in a higher retentive strength.
CNN3 knockdown inhibits the proliferation, invasion and migration of glioma
Authors: Xiao Xu , Wang Yu , Xiao Han , Li Chenlong , Liang Peng 
Published: May 2022

Abstract
BackgroundGlioma is a severe, malignant tumor in the central nervous system. The present study detected the expression level and biological functions of calponin 3 (CNN3) in glioma to investigate the mechanism of glioma. ResultsThe results revealed that CNN3 was significantly overexpressed in glioma cells and tissues, and was associated with poor prognosis. Knockdown of CNN3 reduced the proliferation, invasion and migration of glioma in vitro, and inhibited the growth of glioma in vivo, indicating that CNN3 was significantly associated with MEK/ERK signaling pathway. ConclusionsCNN3 is a tumor-promoting gene in glioma, and a promising novel tumor marker and therapeutic target for glioma diagnosis.
Development of a numerical modelling method to predict the seismic signals generated by wind farms
Authors: Limberger Fabian , Rümpker Georg , Lindenfeld Michael , Deckert Hagen 
Published: May 2022

Abstract
In efforts to reduce greenhouse gas emissions, renewable energies have been increasingly leveraged to generate power; in particular, the number of wind turbines has risen sharply in recent years and continues to grow. However, being mechanically coupled to the earth, wind turbines also generate ground vibrations, which can have adverse effects on the capability of seismic observatories to detect and analyse earthquakes; nevertheless, the distances at which these signals modulate seismic records are disputed between the operators of wind farms and seismic observatories. Here, to quantify the noise signal amplitudes at distant seismometers, we develop the first numerical modelling to predict the seismic wavefield emitted by wind farms and simulate the complex effects of wavefield interferences, surface topography and attenuation. This modelling approach can reliably quantify the influences of multiple wind turbines on ground motion recordings and thus provide necessary information to aid decision-making in advance of wind farm installation.
Exciton density waves in Coulomb-coupled dual moiré lattices
Authors: Zeng Yihang , Xia Zhengchao , Dery Roei , Watanabe Kenji , Taniguchi Takashi , Shan Jie , Mak Kin Fai 
Published: May 2022

Abstract
Strongly correlated bosons in a lattice are a platform to realize rich bosonic states of matter and quantum phase transitions. While strongly correlated bosons in a lattice have been studied in cold-atom experiments, their realization in a solid-state system has remained challenging. Here we trap interlayer excitons--bosons composed of bound electron-hole pairs--in a lattice provided by an angle-aligned WS2/bilayer WSe2/WS2 multilayer; the heterostructure supports Coulomb-coupled triangular moiré lattices of nearly identical period at the top and bottom interfaces. We observe correlated insulating states when the combined electron filling factor of the two lattices, with arbitrary partitions, equals to 1/3,2/3,4/3 and 5/3. These new states can be interpreted as exciton density waves in a Bose-Fermi mixture of excitons and holes. Because of the strong repulsive interactions between the constituents, the holes form robust generalized Wigner crystals, which restrict the exciton fluid to channels that spontaneously break the translational symmetry of the lattice. Our results demonstrate that Coulomb-coupled moiré lattices are fertile ground for correlated many-boson phenomena.
C/EBPβ/AEP Signaling Activated by ApoE4 and 27-Hydroxycholesterol in Macrophages Mediates Cerebral Amyloid Angiopathies in Alzheimer’s Disease
Authors: Liao Jianming , Liu Xia , Chen Guiqin , Wang Zhihao , Gu Xiaohuan , Wei Ling , Yu Shan Ping , Ye Keqiang 
Published: May 2022

Abstract
Cerebral amyloid angiopathy (CAA), amyloid-β (Aβ) deposits in cerebral vessel walls, is commonly associated with Alzheimer’s disease (AD) and contributes to cerebrovascular dysfunctions. APOE4 is a strong genetic risk factor for both AD and CAA. However, the mechanisms underlying this genetic susceptibility remain incompletely understood. Here we show that ApoE4 and 27-hydroxycholesterol (27-OHC) additively drive C/EBPβ/AEP signaling activation in macrophages, which impairs Aβ uptake and clearance and stimulates CAA formation, leading to cerebrovascular dysfunctions and microbleeds. To explore the pathological roles of ApoE4/27-OHC in CAA formation during AD pathogenesis, we crossed smooth muscle cells specific SMA-GFP mice with 5xFAD mice and found reduced blood flow, decreased vessel length and elevated oxidative stress and hemorrhagic lesions, which were exacerbated by ApoE4/27-OHC. Notably, ApoE4/27-OHC promoted Aβ deposits in leptomeningeal, cortical and hippocampal arteries and increased parenchymal Aβ load as compared to ApoE3/27-OHC, associated with strong C/EBPβ/AEP activation in macrophages that surrounded and infiltrated the microvessels. Depletion of C/EBPβ or AEP from 5xFAD/SMA-GFP mice ameliorated ApoE4/27-OHC-triggered CAA-associated vasculopathies and cerebrovascular disorders and senile plaques, alleviating cognitive disorders. Therefore, ApoE4 and 27-OHC jointly facilitate both CAA and AD pathologies via activating C/EBPβ/AEP pathway.
Standard Anterior Peritomy versus a Small Posterior Incision for the Implantation of the PRESERFLO Microshunt
Authors: Bamousa Ahmed , Verma-Fuehring Raoul , Dakroub Mohamad , Papadopoulos Kosmas , Hillenkamp Jost , Loewen Nils A. 
Published: May 2022

Abstract
Purpose: This study aimed at comparing two approaches for the implantation of PRESERFLO microshunts. The standardized approach (A) is an anterior approach that necessitates performing a 6-8 mm perilimbal peritomy. The second approach (P) merely required a 3 mm incision, about 2 mm away from the limbus. Methods: 126 patients, who received PRESERFLO microshunts, were retrospectively examined. Parameters compared included intraocular pressure (IOP), surgical time, medication count and complications. The follow-up duration was 9 months. Results: The baseline IOP was 21.8±8.5 mmHg and 23.9±8.1 mmHg, in A and P, respectively (p=0.08). Surgical duration differed significantly between groups (26±0.8 vs 10±0.4 minutes for A and P, respectively; p<0.001). Postoperative IOP levels were at 10.8±5.9 mmHg in A and 10.6±4.5 mmHg in P at 30 days (p=0.62) and remained thereafter similar throughout the study (all intra-group p-values>0.08). Additionally, no significant differences were recorded between groups at any point in time. Preoperative medication count was 3.2±1.3 drops in A and 3.3±1.0 drops in P(p=0.4). Postoperative values were 0.2±0.6 in A and 0.3±0.7 in P at 9 months. Both groups exhibited similar numbers of complications and surgical revisions (p-values>0.05). Conclusion: Both techniques showed satisfactory IOP and medication count reductions as well as similar safety profiles, with the posterior incision technique being 2.6 times faster than the anterior incision approach.
Effect of transferrin-modified liposomal curcumin on proliferation inhibition of oral squamous cell carcinoma
Authors: Wei Xue-Qin 
Published: May 2022

Abstract
Curcumin can inhibit the proliferation and metastasis of a variety of tumor cells, including oral tumor, but its use is severely limited by its low bioavailability and stability. In this study, transferrin-modified liposomal curcumin was constructed to detect its inhibition on oral squamous cell carcinoma cell line HN4 and compared with liposomal curcumin and curcumin solution (curcumin dissolved in PBS (pH7.4, 0.01Mol/L)). Firstly, curcumin solution (Cur), liposomal curcumin (Cur-Lips) and transferrin-modified liposomal curcumin (Tf-Cur-Lips) were prepared. The regulatory effects of Cur, Cur-Lips and Tf-Cur-Lips on the internal and external disposal of curcumin were investigated by curcumin release in vitro and pharmacokinetics in vivo in rats. Then, HN4 cells were treated with Cur, Cur-Lips and Tf-Cur-Lips at different concentrations, respectively, and cck-8 was used to detect the effect of different experimental groups on HN4 cells proliferation. Finally, to investigate the molecular mechanism of curcumin inhibiting the proliferation and apoptosis of HN4 cells, the expression levels of apoptosis-related genes P53 and Fas was detected and compared by real-time fluorescence quantitative PCR in Cur, Cur-Lips and Tf-Cur-Lips groups. We found that compared with Cur, Cur-Lips metabolic time was significantly prolonged, and transferrin modification could further improve the stability of liposomal curcumin and prolongate liposomal curcumin metabolic time. Compared with Cur and Cur-Lips, Tf-Cur-Lips can significantly enhance the proliferation inhibition of HN4 cells, induce their apoptosis, and up-regulate the expression of apoptosis-related genes P53 and Fas. In conclusion, compared with Cur and Cur-Lips, Tf-Cur-Lips have stronger inhibitory effect on oral squamous cell carcinoma cell line HN4. This study will provide a new option for construction a long-term targeted curcumin delivery system against oral squamous cell carcinoma.
Weakened AMOC upper limb compensated by strengthened South Atlantic subtropical gyre circulation in CESM1-LE simulations
Authors: Marcello Fernanda , Tonelli Marcos , Ferrero Bruno , Wainer Ilana 
Published: May 2022

Abstract
The upper limb of the Atlantic Meridional Overturning Circulation (AMOC) brings shallow interocean contributions to replenish the North Atlantic export of deepwaters. It is primarily formed in the southern South Atlantic where the converging entrainment of Pacific and Indian Ocean waters meet and incorporate into the South Atlantic subtropical gyre (SASG) following a northward advection across the basin up to the western boundary. Here, the human-induced response of AMOC and SASG near-surface pathways are illustrated according to CESM1 Large Ensemble simulations from 1920 to 2100, where future projections derive from the most aggressive (yet most realistic) scenario in assumed fossil fuel use and greenhouse gas emissions. It is shown that the AMOC upper limb weakens not because less waters are being apported from the adjacent ocean basins — but because they are being mostly directed to recirculate in the anticlockwise SASG, turning back southward after reaching the South Atlantic western boundary.