bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autistic spectrum disorders: genetic and epigenetic aspects (review of modern foreign publications)
Authors:   Kherson State University, Lanovenko O. 
Published: February 2022

Autism spectrum disorders (ASD) are diseases of a complex interaction of heredity and the environment with an estimate of heritability from 40 to 80 %, the etiology of which is multigenic and heterogeneous (people with similar pathogenetic variants have completely different phenotypes). Genetic and epigenetic factors play a key role in the onset of pathology; among them, the most important are gene copy number variations (CNV), single nucleotide polymorphisms (SNP), epigenetic modulators. Different variations in gene copy numbers have been identified in people with autism, which explains the heterogeneous genetic architecture of the disorder. Short single nucleotide polymorphisms have been identified in the genes of neuronal receptors and proteins of cell detoxification, the metabolism of neurotransmitters and metabolites, in particular, the metabolic chains of methylation and transulfuration. Despite the identification of about 800 risk genes for ASD, none of them is the cause of more than 1 % of all cases of pathology. Among the recently identified 215 candidate genes, 19.5 % are epigenetic regulators. During the formation of autism spectrum disorders, nuclear and cytoplasmic mechanisms regulating gene activity undergo epigenetic transformation: chromatin remodeling, transcription, DNA methylation in the promoter region, alternative mRNA splicing, translation regulation, post-transcriptional regulation using microRNA, protein ubiquitination, protein degradation. Mutant proteins encoded by candidate ASD genes interfere with normal cell proliferation, correct synaptic architecture, and correct synaptic function. Most often, people with autism have inherited changes with incomplete penetrance, variable severity, or both. The most studied environmental risk factors that potentially contribute to the onset of ASD in offspring are: the age of the spouses (especially the father), perinatal risk factors, impaired metabolism of vitamins (in particular, vitamin D) and trace elements (zinc, chromium, magnesium), obesity, and Diabetes mellitus in the mother ... In the urine of children with autism, the content of dopamine is significantly increased, and the normal microflora in the intestine is disrupted as a result of replacing beneficial probiotics with clostridium bacteria. Given the complexity of the etiology of autism and the growing prevalence of new confirmed cases of ASD around the world, there is an urgent need to find effective diagnostic methods and identify the main risk factors for pathology.
Transformation processes in alpine phytocoenoses of the Ukrainian Carpathians under reservation and climate changes
Authors:   Ivan Franko National University of Lviv, Kyyak V. , Shtupun V. ,   Institute of Ecology of the Carpathians, NAS of Ukraine
Published: February 2022

Typycal alpine phytocoenoses of Ukrainian Carpathians with dominant species Festuca supina, Carex curvula, Juncus trifidus, Vaccinium uliginosum, Rhododendron myrtifolium, Sesleria coerulens and open chionophilous communities were investigated. As a result of comparative studies during 1982–2021, among alpine phytocoenoses, the smallest changes in the structure, abundance and coenose-forming positions of species were found in communities dominated by Juncus trifidus, Vaccinium uliginosum, Rhododendron myrtifolium and Sesleria coerulans, in particular in the Juncetum cetrario-myrtillosum, Uliginetum cetrariosum, Rhodoretum calamagrostiosum and Seslerietum cariceto-festucosum communities. During the same period in communities with dominant Festuca supina and Carex curvula, the position of the dominants decreased significantly. The Festuca supina-communities undergo desintegration and replacement by other phytocoenoses with dominance Juniperus alpinа, Vaccinium myrtillus, V. vitis-idaea, V. uliginosum and Calamagrostis villosum. Over the last decade, radical changes have taken place in Carex curvula-communities. In contrast to the decisive influence of postpastoral demutation processes on the degradation of Festuca supina-communities, changes in the phytocoenoses of Carex curvula are prima­rily climatogenically caused. It is established that in the alpine zone Festuca supina-communities do not belong to the indigenous primary phytocoenoses, but are secondary pastorally conditioned derivative succession stages of primary communities of other associations. Rapid positive dynamics over the last ten years of such mesochionophilous species as Veronica alpina, Gnaphalium supinum, Soldanella hungarica, Luzula spadicea and, at the same time, negative changes in populations of obligate chionophiles Cerastium cerastoides and Saxifraga carpatica indicate transformation processes within the communities connected to the areas under long and deep snow cover. These processes indicate rapid successional changes under the influence of increa­sing vegetation period due to the reduction of the duration of snow cover in the territory of chionophilous phytocoenoses, which is due to modern climate changes, primarily warming.
ANALISIS TECHNOLOGY ACCEPTANCE MODEL (TAM) PADA INTENTION TO TRANSACT ONLINE (STUDI KASUS: E-WALLET OVO DI KOTA SURABAYA)
Authors: Nainggolan Blandina Angelina , Andrian David 
Published: February 2022

Digital payment mengalami pertumbuhan yang sangat pesat sejak awal kehadirannya di Indonesia. Digital payment merupakan inovasi dalam industri jasa finansial yang memanfaatkan teknologi sehingga memungkinkan penggunanya melakukan berbagai transaksi keuangan secara digital. Salah satu digital payment yaitu e-wallet OVO yang dikelola oleh PT. Visioner Internasional yang memberikan kemudahan dalam bertransaksi (OVO Cash) dan memberi kesempatan lebih besar dalam mengumpulkan poin (OVO Points). Saat ini aplikasi OVO dapat diakses melalui smartphone baik pengguna android maupun iOS.
Tujuan dari penelitian ini adalah mengetahui pengaruh variabel perceived case to use, perceived usefulness, act to use, perceived trust, perceived risk terhadap intention to transact online pada penggunaan e-wallet OVO. Analisis yang digunakan untuk penelitian ini adalah analisis deskriptif dan analisis Partial Least Square (PLS). Pengumpulan data dilakukan dengan menyebarkan kuesioner kepada minimum 385 responden pengguna e-wallet OVO di Surabaya. Untuk memudahkan penulis, hasil penelitian diolah dengan software SmartPLS 3.0.
Berdasarkan hasil analisis, variabel perceived usefulness dan act to use berpengaruh positif signifikan, sedangkan variabel perceived ease of use, perceived trust, dan perceived risk berpengaruh positif namun tidak signifikan terhadap intention to transact online. Dan dalam penelitian ini menjelaskan 54% dari intention to transact online pada pengguna e-wallet di Kota Surabaya.
Transformasi Digital pada Budaya Tradisi Menenun Endek
Authors: Dewi Pebryani Nyoman , Ratna C.S Tjok Istri , Rai Remawa Anak Agung , Radiawan I Made 
Published: February 2022

Endek adalah tekstil tradisional yang diproduksi di Pulau Bali dengan menggunakan teknik single ikat, motif pada benang dihasilkan dengan cara mengikat benang pakan pada motif yang diinginkan. Tenun Endek merupakan salah satu budaya yang diwariskan dari generasi ke generasi. Menghasilkan motif pada Endek, membutuhkan kreatifitas dan proses perhitungan yang cukup rumit, sehingga tidak banyak orang yang menguasai kemampuan membuat motif dan menghitung benang secara bersamaan. Salah satu cara untuk memudahkan penenun untuk mendesain sekaligus menghitung benang adalah dengan menciptakan sebuah aplikasi yang mampu menyediakan kemampuan untuk mendesain sekaligus menghitung jumlah benang yang dibutuhkan, sehingga pengguna hanya fokus untuk mendesain motif. Untuk memahami algoritma pembuatan tenun Endek, maka peneliti melakukan penelitian di lapangan terlebih dahulu. Setelah mendapatkan variabel pembuatan tenun Endek dari lapangan, kemudian variabel tersebut diterjemahkan kedalam bahasa pemrograman untuk membentuk aplikasi. Aplikasi ini diciptakan dengan user interface yang sederhana agar mudah digunakan bagi orang yang tidak familiar dengan teknologi. Penciptaan aplikasi ini berkontribusi pada efisiensi pembuatan motif pada pertenunan yang biasanya dilakukan dalam satu hingga dua hari, dengan adanya aplikasi ini mampu mempersingkat waktu pembuatan desain menjadi satu hingga dua jam.
Awareness of Yoga and Its Benefit Among Medical Students of Tertiary Care Teaching Hospital in Birgunj
Authors: Shrestha Esha , Shreshtha Ripti , Ghimire Dayaram , Aryal Vibina 
Published: February 2022

Introduction
Yoga is a practice of mind and body that combines physical postures, breathing exercises, and meditation. Yoga helps reduce stress, lower blood pressure and heart rate. It is considered one of many complementary and integrative health approaches. Yoga brings together physical and mental discipline that may help achieve a healthy body and peaceful mind. In this study, we aim to assess knowledge and awareness regarding yoga and its benefit among preclinical students.
Materials and Methods
This cross-sectional study includes 176 preclinical medical students of National Medical College, Birgunj, Nepal. A total of 14 self-designed close-ended questionnaires regarding knowledge and awareness about Yoga were used as a study tool. Data analysis was done using Microsoft Excel, 2010.
Results
Among 176 preclinical students, 77.84% had the habit of practicing yoga, but only 14.20% of students continued it. About 96.59% of students knew the benefits of yoga.
Conclusion
The result of the study reveals that there is a positive influence of practicing yoga among students. By practicing yoga, it is capable to achieve personal abilities such as stress management, attention in their work, and maintenance of physical and mental health.
Efektivitas Layanan Hak Tanggungan Terintegrasi Secara Elektronik Di Kantor Pertanahan Kabupaten Klaten
Authors: Sagari Damar , Mujiati Mujiati 
Published: February 2022

Modernisasi pelayanan pertanahan merupakan keniscayaan agar dapat menyelesaikan pekerjaan secara cepat, tepat, dan efektif. Kementerian ATR/BPN mewujudkan tuntutan tersebut salah satunya melalui pelayanan Hak Tanggungan terintegrasi secara elektronik (HT-el) yang wajib diterapkan oleh seluruh Kantor Pertanahan mulai tanggal 8 Juni 2020. Berdasarkan hal tersebut Kantor Pertanahan Kabupaten Klaten menyelenggarakan layanan HT-el sejak Januari 2020 untuk mempermudah mitra Kementerian ATR/BPN dalam memperoleh pelayanan secara online. Tujuan penelitian untuk mengetahui efektivitas pelaksanaan Layanan HT-el serta faktor pendukung dan penghambat Layanan HT-el di Kantor Pertanahan Kabupaten Klaten. Metode kualitatif untuk menguraikan efektivitas layanan HT-el di Kantor Pertanahan Kabupaten Klaten. Hal itu dimaksudkan agar permasalahan yang terindikasi dapat menurunkan kualitas pelayanan dan kepuasan masyarakat dapat diperbaiki. Hasil penelitian menunjukkan bahwa efektivitas layanan HT-el di Kantor Pertanahan Kabupaten Klaten memperoleh respons yang baik. Efektivitas layanan tersebut tidak terlepas dari faktor pendukung dan penghambat yang mempengaruhi pelaksanaannya.
Pengaruh Campuran Pelet Kudzu Dengan Karbohidrat Terhadap Protein Total, Urea, Glukosa dan Kolesterol Darah Domba Ekor Tipis
Authors: Ramdhan Muhamad - , Susilawati Iin , Mayasari Novi 
Published: February 2022

This research aimed to determine blood protein total, blood urea, blood glucose and blood cholseterol on thin tail rams which is administered kudzu pellet with readily available carbohydrates. Experimental method used completely randomize design. The data was analyzed through Anova and Duncan’s Multiple Range Test. Twenty four local rams are divided into four dietary treatment, namely RK = 50 % napier grass + 50 % concentrate, KD = 50 % napier grass + 25 % kudzu pellet with rice bran + 25 % concentrate, KM = 50 % napier grass + 25 % kudzu pellet with molasses + 25 % concentrate, KG = 50 % napier grass + 25 % kudzu pellet dried cassava + 25 % concentrate. Each treatment was replicated six times. Administering dried cassava (KG) in kudzu showed significance (P<0,05) blood glucose in thin-tailed ram compared to control dietary (RK) and bran rice kudzu pellet (KD) based on table 2. Administering dried cassava (KG) and molasses (KM) in kudzu pellet provided similar response (Table 2) on blood glucose level. Blood cholesterol level decreased along with the duration of treatment (Table 2) during 60 days compared before treatment. The result showed that duration of treatment made a significant decrease (P<0,05) (Table 2) on blood cholesterol in rams. Blood total protein declined when the treatment was administered during 60 days. The result explained that duration of treatment is significant (P<0,05) to decrease blood total protein level on rams (Table 2). The blood urea level of this research showed that the duration of treament is significant (P<0,05) to increase blood urea level on thin-tailed rams (Table 2). Blood urea level on each treatment was range between 31,23 mg/dL – 34,57 mg/dL. This result explained that the increase of blood urea level showed a significant difference (P<0,05) when diet treatments interacted with the duration of treatment. The conclusion of this research was administering dried cassava (KG) and molasses (KM) in kudzu pellet increase blood glucose level, stabilized blood total protein, urea and cholesterol in thin-tailed rams. However, the protein balance in thin-tailed rams diet on different age should be considered in order to support the growth. Keywords: Kudzu, Blood Urea, Blood Glucose, Blood Protein Total, Blood Cholesterol
Scaffold Geometry-Imposed Anisotropic Mechanical Loading Guides the Evolution of the Mechanical State of Engineered Cardiovascular Tissues in vitro
Authors: Hermans L. H. L. , Van Kelle M. A. J. , Oomen P. J. A. , Lopata R .G. P. , Loerakker S. , Bouten C. V. C. 
Published: February 2022

Cardiovascular tissue engineering is a promising approach to develop grafts that, in contrast to current replacement grafts, have the capacity to grow and remodel like native tissues. This approach largely depends on cell-driven tissue growth and remodeling, which are highly complex processes that are difficult to control inside the scaffolds used for tissue engineering. For several tissue engineering approaches, adverse tissue growth and remodeling outcomes were reported, such as aneurysm formation in vascular grafts, and leaflet retraction in heart valve grafts. It is increasingly recognized that the outcome of tissue growth and remodeling, either physiological or pathological, depends at least partly on the establishment of a homeostatic mechanical state, where one or more mechanical quantities in a tissue are maintained in equilibrium. To design long-term functioning tissue engineering strategies, understanding how scaffold parameters such as geometry affect the mechanical state of a construct, and how this state guides tissue growth and remodeling, is therefore crucial. Here, we studied how anisotropic versus isotropic mechanical loading—as imposed by initial scaffold geometry—influences tissue growth, remodeling, and the evolution of the mechanical state and geometry of tissue-engineered cardiovascular constructs in vitro. Using a custom-built bioreactor platform and nondestructive mechanical testing, we monitored the mechanical and geometric changes of elliptical and circular, vascular cell-seeded, polycaprolactone-bisurea scaffolds during 14 days of dynamic loading. The elliptical and circular scaffold geometries were designed using finite element analysis, to induce anisotropic and isotropic dynamic loading, respectively, with similar maximum stretch when cultured in the bioreactor platform. We found that the initial scaffold geometry-induced (an)isotropic loading of the engineered constructs differentially dictated the evolution of their mechanical state and geometry over time, as well as their final structural organization. These findings demonstrate that controlling the initial mechanical state of tissue-engineered constructs via scaffold geometry can be used to influence tissue growth and remodeling and determine tissue outcomes.
Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances
Authors: Ssekatawa Kenneth , Byarugaba Denis K. , Angwe Martin Kamilo , Wampande Eddie M. , Ejobi Francis , Nxumalo Edward , Maaza Malik , Sackey Juliet , Kirabira John Baptist 
Published: February 2022

The greatest challenge of the current generation and generations to come is antimicrobial resistance, as different pathogenic bacteria have continuously evolved to become resistant to even the most recently synthesized antibiotics such as carbapenems. Resistance to carbapenems limits the therapeutic options of MDR infections as they are the only safe and effective drugs recommended to treat such infections. This scenario has complicated treatment outcomes, even to the commonest bacterial infections. Repeated attempts to develop other approaches have been made. The most promising novel therapeutic option is the use of nanomaterials as antimicrobial agents. Thus, this study examined the efficacy of Camellia sinensis extract (CSE) and Prunus africana bark extract (PAE) green synthesized Copper oxide nanoparticles (CuONPs) against carbapenem-resistant bacteria. Furthermore, the photocatalytic and antioxidant activities of CuONPs were evaluated to determine the potential of using them in a wide range of applications. CuONPs were biosynthesized by CSE and PAE. UV vis spectroscopy, X-ray Diffraction (XRD), Dynamic light scattering (DLS), Fourier Transform Infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM) were used to characterize the nanoparticles. CuONPs susceptibility tests were carried out by the agar well diffusion method. The photocatalytic and antioxidant activities of the CuONPs were determined by the methylene blue and DPPH free radical scavenging assays, respectively. UV vis absorbance spectra registered surface plasmon resonance peaks between 272 and 286 nm, confirming the presence of CuONPs. The XRD array had nine strong peaks at 2θ values typical of CuONPs. FTIR spectra exhibited bands associated with organic functional groups confirming capping and functionalization of the CuONPs by the phytochemicals. DLS analysis registered a net zeta potential of +12.5 mV. SEM analysis revealed that the nanoparticles were spherical and clustered with a mean diameter of 6 nm. Phytosynthesized CuONPs exhibited the highest growth suppression zones of 30 mm with MIC ranging from 30 to 125 μg/ml against MDR bacteria. Furthermore, the CuONPs achieved a methylene blue dye photocatalysis degradation efficiency of 85.5% and a free radical scavenging activity of 28.8%. PAE and CSE successfully bio-reduced copper ions to the nanoscale level with potent antimicrobial, photocatalysis, and antioxidant activities.
Spectroscopy, Morphology, and Electrochemistry of Electrospun Polyamic Acid Nanofibers
Authors: Hamnca Siyabulela , Chamier Jessica , Grant Sheila , Glass Timothy , Iwuoha Emmanuel , Baker Priscilla 
Published: February 2022

Polyamic acid (PAA) nanofibers produced by using the electrospinning method were fully characterized in terms of morphology and spectroscopy. A PAA nanofiber–modified screen-printed carbon electrode was applied to the detection of selected sulfonamides by following an electroanalytical protocol. The polyamic acid (PAA) nanofibers were characterized using Fourier transform infrared (FTIR) spectroscopy to study the integrity of polyamic acid functional groups as nanofibers by comparing them to chemically synthesized polyamic acid. A scanning electron microscope (SEM) was used to confirm the morphology of the produced nanofibers and 3D arrangement at the electrode interface. The Brunauer–Emmett–Teller (BET) method was used to determine the surface area of the nanofibers. Atomic force microscopy (AFM) was used to study the porosity and surface roughness of the nanofibers. Electrochemical evaluation based on diffusion-controlled kinetics was applied to determine the number of electrons transferred in the system, the surface concentration of the deposited PAA thin film (2.14 × 10−6 mol/cm2), and the diffusion coefficient (De) for the PAA nanofiber–modified screen-printed carbon electrode (9.43 × 10−7 cm−2/s). The reported LODs for sulfadiazine and sulfamethazine detection are consistent with requirements for trace-level monitoring by early warning diagnostic systems.
The Beneficial Effects of Beta Blockers on the Long-Term Prognosis of Patients With Premature Atrial Complexes
Authors: Huang Ting-Chun , Lee Po-Tseng , Huang Mu-Shiang , Chiu Pin-Hsuan , Su Pei-Fang , Liu Ping-Yen 
Published: February 2022

AimsPremature atrial complexes (PACs) have been reported to increase the risk of adverse cardiovascular outcomes. Beta blockers at low dosages may help to reduce PAC symptoms, but it is unclear whether they can improve long-term outcomes.MethodsPatients enrolled from a Holter cohort in a medical referral center were stratified into high-burden (≥100 beats/24 h) and low-burden (&lt;100 beats/24 h) sub-cohorts, and propensity score matching between treatment groups and non-treatment groups was conducted for each sub-cohort.ResultsIn the high-burden sub-cohort, after propensity score matching, the treatment group and non-treatment group respectively had 208 and 832 patients. The treatment group had significantly lower mortality rates than the non-treatment group [hazard ratio (HR) = 0.521, 95% confidence interval (CI) = 0.294–0.923, p = 0.025], but there was no difference in new stroke (HR = 0.830, 95% CI = 0.341–2.020, p = 0.681), and new atrial fibrillation (HR = 1.410, 95% CI = 0.867–2.292, p = 0.167) events. In the low-burden sub-cohort, after propensity score matching, there were 614 patients in the treatment group and 1,228 patients in the non-treatment group. Compared to the non-treatment group, up to 40% risk reduction in mortality was found in the treatment group (HR = 0.601, 95% CI = 0.396–0.913, p = 0.017), but no differences in new stroke (HR =0.969, 95% CI = 0.562–1.670, p = 0.910) or atrial fibrillation (HR = 1.074, 95% CI = 0.619–1.863, p = 0.800) were found.ConclusionsBeta blockers consistently decreased long-term mortality in high-burden and low-burden patients. Interestingly, this effect was not achieved through reduction of new-onset stroke or AF, and further research is warranted.