bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fertility and pregnancy outcomes in women with nonclassic 21‐hydroxylase deficiency
Authors: Carrière Camille , Nguyen Lee S. , Courtillot Carine , Tejedor Isabelle , Chakhtoura Zeina , Bellanné‐Chantelot Christine , Tardy Véronique , Leban Monique , Touraine Philippe , Bachelot Anne 
Published: November 2022

AbstractIntroductionOverall fertility and pregnancy outcomes in patients with nonclassic congenital adrenal hyperplasia (NCCAH) have been poorly studied. It has been suggested that hydrocortisone (HC) may decrease the time to conceive (TTC) and the rate of miscarriage in these patients.ObjectivesTo describe fertility and pregnancy outcomes in a large cohort of NCCAH women. The secondary objective was to identify factors that could impact reproductive outcomes, with a particular focus on HC dose and genetic status.DesignRetrospective study in a referral center for congenital adrenal hyperplasia.Patients and MeasurementsOne hundred seventy‐three female patients with NCCAH confirmed by genetic testing, followed in our center between 2010 and 2019.ResultsAmong the 173 patients, 95 women had a parental project, 86 of whom presented 176 pregnancies, 56% under glucocorticoid (GC) treatment and 44% without, and 76 women obtained 128 live births. Two‐thirds of the patients regularized their cycle under GC treatment, with significant decrease of androgens and progesterone levels. This treatment was associated with a shortening of TTC (coef β = −.196, information coefficient [IC] = [−10.7; −0.91], p = .021). Androgen levels and TTC were positively correlated to the rate of miscarriage (OR = 4.8, IC = [1.15; 20.34], p = .021 for testosterone, OR = 1.4, IC = [1.05; 1.81], p = .02 for androstenedione, and OR = 1.03, IC = [1.01; 1.06], p = .015 for TTC). There was no difference in terms of obstetric outcomes between patients with or without GC treatment. CYP21A2 genotype had no impact on pregnancy outcome or TTC.ConclusionsInfertility is relative in patients with NCCAH. HC seems beneficial for fertility and pregnancy outcomes, especially for patients with menstrual disorders and high preconceptional androgen levels.
Ribavirin aerosol in hospitalized adults with respiratory distress and COVID‐19: An open‐label trial
Authors: Poulakou Garyfallia , Barakat Maxime , Israel Robert J. , Bacci Marcelo R. ,   Virazole Collaborator Group for COVID‐19 Respiratory Distress
Published: November 2022

AbstractThere is an unmet medical need for effective treatments for hospitalized patients with coronavirus disease 2019 (COVID‐19). Ribavirin is a broad‐spectrum antiviral with demonstrated in vitro activity against multiple viruses, including severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). This trial evaluated the potential of ribavirin inhalation solution (ribavirin aerosol) to reduce COVID‐19 disease severity in adults with confirmed SARS‐CoV‐2 infection and a diagnosis of respiratory distress. This phase I, multicenter, open‐label, nonrandomized trial was conducted from February 2021 through August 2021. Patients received ribavirin aerosol (100 mg/ml for 30 min or 50 mg/ml for 60 min) twice daily for up to 6 days. The primary end point was change from baseline in clinical status severity, rated on a 7‐point scale (1 [death]; 7 [not hospitalized; no limitations on activities]), at day 7 (or end‐of‐treatment/early termination) and day 30 (follow‐up). Fifty‐one patients were treated with ribavirin aerosol (mean age, 51.5 years; 78.4% men); mean number of doses was 9.7 (range, 1–12). Improvement of ≥1 level in clinical status severity was observed in 31.4% (16/51) and 78.4% (40/51) of patients at end‐of‐treatment and day 30, respectively. Of 21 patients who required a ventilator, 16 (76.2%) were able to discontinue ventilator use. Five patients (9.8%) died between end‐of‐treatment and day 30. Three patients (5.9%) discontinued study treatment due to adverse events. No deaths were considered related to study treatment. These data provide preliminary evidence that ribavirin aerosol may be an efficacious treatment for respiratory distress in adults with COVID‐19.
Complex sweat gland hamartoma: A case report
Authors: Skopec Zachary , Berrebi Kristen G. , Stone Mary , Liu Vincent 
Published: November 2022

AbstractSyringocystadenoma papilliferum (SCAP), tubular apocrine adenoma (TAA), and eccrine nevus are rare benign sweat gland tumors with varied clinical presentations but generally distinctive histomorphologic profiles. TAA and SCAP have been associated with other cutaneous hamartomas, most commonly with nevus sebaceus. Additionally, TAA and SCAP have uncommonly co‐occurred in the same lesion. In contrast to nevus sebaceus, eccrine nevus is considerably less common and is rarely associated with other benign adnexal lesions. Here we present an unusual case of a complex sweat gland hamartoma containing features of syringocystadenoma papilliferum, tubular apocrine adenoma, and eccrine nevus in a 7‐year‐old female.
Toward the articulation of mission: Heeding the ELCA's call for affordable mental health care and authentic diversity while guiding students' journeys toward counseling and clinical vocations
Authors: Holigrocki Richard 
Published: November 2022

AbstractI describe community counseling clinics embedded with a Lutheran University and aligned with Evangelical Lutheran Church in America (ELCA) values. Our clinics, like many others, serve the underserved and are often dependent upon external support. To ensure operational stability, they must articulate their mission alignment with host institutions and funders. In so doing, funders readily see how their values are enacted and embodied within the supported program. Examples of mission alignment, data supporting alignment, and the benefits of alignment for the program, funder, and those served are provided.
Cerebellar involvement associated with immune checkpoint inhibitors: A systematic review
Authors: Dinoto Alessandro , Mantovani Elisa , Ferrari Sergio , Mariotto Sara , Tamburin Stefano 
Published: November 2022

AbstractBackground and purposeImmune checkpoint inhibitors (ICIs) targeting programmed death receptor 1 (PD‐1), cytotoxic T‐lymphocyte‐associated‐4 (CTLA‐4) and programmed cell death ligand 1 can be associated with immune‐related adverse events (iRAEs). Amongst neurological iRAEs, cerebellar involvement seems to be rare and currently lacks a proper characterization. The aim of this study was to phenotype cerebellar iRAEs.MethodsA systematic review was performed according to PRISMA guidelines including reported patients with cerebellar involvement related to ICIs and with available individual data.ResultsAfter screening 2765 records, 32 studies with 46 patients were included. Median age was 63 years (20–82), and most patients were male (63.0%). Isolated cerebellitis was observed in 32.6% of cases, whilst the remaining cases had “cerebellitis plus”, mostly associated with encephalitis/encephalopathy. Associated tumors included most frequently lung cancer, melanoma and Merkel cell carcinoma. PD‐1 inhibitor was the most administered treatment (n = 29, 64.4%), whilst exposure to CTLA‐4 inhibitor was rare (n = 2, 4.5%). Magnetic resonance imaging was abnormal in 43.2% of patients and inflammatory cerebrospinal fluid findings were frequently observed. Autoantibodies were detected in 61.9% of patients and included novel reactivities. Amongst treatment strategies, the most common were steroids (n = 36) and ICI discontinuation (n = 28, 90.3%). Relapses were reported in 10% of patients. Most patients showed improvement/remission (n = 31) but, at last follow‐up, 12 had died. Isolated cerebellitis versus cerebellitis‐plus differed in terms of outcomes, whilst seropositive versus seronegative patients had distinct tumor associations.DiscussionCerebellar iRAEs are usually multifocal, have heterogeneous tumor associations, are most associated with PD‐1 inhibitor exposure and are related to autoantibodies, including novel reactivities.
Association between subjective cognitive complaints, balance impairment and disability among middle‐aged and older adults: Evidence from a population‐based cohort study
Authors: Tu Raoping , Wang Suhang , He Huihui , Ding Jiali , Zeng Qingping , Guo Lu , Li Yueping , Xu Tianwei , Lu Guangyu 
Published: November 2022

AimTo quantify the association between subjective cognitive complaints and balance impairment in relation to the occurrence of disability.MethodsIn total, 6885 adults aged ≥45 who participated in the China Health and Retirement Longitudinal Study (CHARLS) were followed for 7 years. Subjective cognitive complaints were evaluated by self‐reported memory problems. Balance impairment was tested by side‐by‐side stand, semi‐tandem stand and full tandem stand. Disability was measured by activities of daily living (ADL) and instrumental activities of daily living (IADL). Multivariate logistic regression models were applied to test the joint effect between baseline subjective cognitive complaints and balance impairment on disability. The multiplicative interaction was examined.ResultsA joint effect of experiencing both subjective cognitive complaints and balance impairment was identified, showing a 1.63‐fold higher risk of ADL and IADL disability than those experienced by neither of the two (odds ratio = 1.63, 95% confidence interval: 1.36–1.95). There was evidence of multiplicative interaction (P = 0.004).ConclusionsAmong middle‐aged and older people, the coexistence of subjective cognitive complaints and balance impairment may lead to a higher disability risk, which is much higher than the simple sum of the two individual effects. Future interventions are required to target these symptoms simultaneously to reduce the risks of disability. Geriatr Gerontol Int 2022; 22: 1025–1031.
Effect of different postmortem ageing conditions on physicochemical properties, structure and water‐holding capacity of pork
Authors: Zhang Handan , Cao Yungang , Dong Xinling , Li Xia , Zhang Chunhui 
Published: November 2022

SummaryThis study aimed to determine the effects of postmortem ageing temperatures on protein–water interactions and muscle water‐holding capacity (WHC) of pork. The oxidation degree, solubility and secondary structure of myofibrillar protein (MP) were also studied. Porcine longissimus lumborum muscles were aged at −6 ± 1, 4 ± 1, and 10 ± 1°C for 3, 12, 24, 72, 120 and 168 h, respectively. The results showed the lower ageing temperature is conducive to decrease the protein oxidation and the transformation of protein conformation from ordered structures (α‐helices and β‐sheets) to disordered structures (β‐turns and random coils). These physicochemical changes favoured protein–water interactions, thus the muscle samples aged at −6 ± 1°C for 120 h had the highest protein solubility and WHC. This work revealed that ageing at sub‐freezing temperature (−6 ± 1°C) is beneficial to reduce protein oxidation and improve muscle water‐holding capacity.
Protein hydrolysate from salmon frame debittered by plastein reaction: amino acid composition, characteristics and antioxidant activities
Authors: Sharma Kartik , Nilsuwan Krisana , Zhang Bin , Hong Hui , Benjakul Soottawat 
Published: November 2022

SummaryImpacts of plastein reaction on bitterness, physicochemical and antioxidant properties of salmon frame hydrolysate with the aid of various proteases (alcalase and papain) at different concentrations and varying reaction temperatures were investigated. Plastein was produced from hydrolysate by papain at 40°C, which had 30% degree of hydrolysis (30DHP). Rearrangement of peptides in hydrolysate was performed by 1% papain at 40°C for 10 h, yielding plastein namely ‘30DHP‐P1’. It showed the lowest bitterness (P < 0.05) than other plasteins and hydrolysates. Surface hydrophobicity was not related well with bitterness. Therefore, the size of peptides also determines the bitterness. 30DHP‐P1 had augmented solubility; however, its antioxidant activities (DPPH and ABTS radical scavenging activities and ferric reducing antioxidant power) were slightly lower (P < 0.05) than those of hydrolysates. Bitterness of hydrolysate was markedly debittered via plastein reaction under optimal condition. Plastein generally had lighter colour and still possessed antioxidant activity.
Clinical in‐situ evaluation of the effect of rubber dam isolation on bond strength to enamel
Authors: Falacho Rui I. , Melo Eliana Azevedo , Marques Joana A. , Ramos João Carlos , Guerra Fernando , Blatz Markus B. 
Published: November 2022

AbstractObjectiveTo evaluate the effect of rubber dam isolation on shear bond strength of two different adhesive systems to enamel.Materials and MethodsThe mesial, distal, lingual, and vestibular enamel surfaces of thirty human third molars were prepared (total n = 120). A custom splint was made to fit a volunteer's maxilla, holding the specimens in place in the oral cavity. Four composite resin cylinders were bonded to each tooth with one of two bonding agents (OptiBond FL and Prime&Bond active) with or without rubber dam isolation. Shear bond strength was tested in a universal testing machine and failure modes were assessed. Significance level for statistical analyses was set at 5%.ResultsAll pairwise comparisons revealed statistical differences (p < 0.05). The highest mean shear bond strength values were obtained in rubber dam experimental groups, regardless of the adhesive system. Group OptiBond FL with rubber dam presented the highest mean bond strength values. Fracture modes for specimens bonded without rubber dam isolation were adhesive and cohesive within enamel, while rubber dam experimental groups revealed only cohesive fractures.ConclusionsAbsolute isolation with rubber dam increases bond strength to enamel, independent of the adhesive system. The three‐step total‐etch system OptiBond FL provided significantly higher bond strength values than Prime&Bond active under both experimental conditions.Clinical SignificanceRubber dam isolation has a significant effect on bond strengths to enamel, independent of the adhesive system. Its application is, therefore, advised whenever adhesive procedures are performed. A filled three‐step etch‐and‐rinse adhesive performed superiorly, with or without rubber dam isolation, when bonding to enamel compared to an isopropanol‐based universal adhesive.
Distinct phenotypic clusters of sleep‐disordered breathing and their association with medical care‐seeking behaviour and sleep habits: the Guangdong Sleep Health Study
Authors: Wang Longlong , Ou Qiong , Shan Guangliang , Lao Miaochan , Xu Yanxia , Pei Guo 
Published: November 2022

SummarySleep‐disordered breathing (SDB) is highly prevalent, but its rates of diagnosis and treatment are low. As a heterogeneous condition, it is associated with delayed diagnosis and/or suboptimal treatment. We aimed to determine distinct SDB clusters and examine their association with medical care‐seeking behaviour and sleep habits in a community‐dwelling population in South China. Participants were enrolled in the ‘Guangdong Sleep Health Study’. Five distinct community sites were investigated. Participant information was collected, and overnight sleep monitoring was performed. Latent class analysis was performed to classify patients with SDB based on patterns of SDB‐related symptoms and signs. Overall, 1,524 patients with SDB were evaluated. Four distinct clusters were identified: ‘minimally symptomatic’ (Cluster 2), which was the most dominant subtype (41.6%), followed by ‘pure insomnia/fewer daytime symptoms’ (Cluster 4; 24.7%), ‘insomnia/multiple daytime symptoms’ (Cluster 3; 17.8%), and ‘upper airway symptoms with sleepiness’ (Cluster 1; 15.9%). The overall medical care‐seeking rate attributable to sleep and breathing disorders was only 3.3%: 10.3% in Cluster 3, 2.5% in Cluster 1, 2.1% in Cluster 4, and 1.3% in Cluster 2, in which Cluster 3 was the highest and Cluster 2 was the lowest (adjusted p < 0.05). Regarding the sleep habit of going to bed and waking up at a consistent time, Cluster 3 exhibited the worst performance and Cluster 2 the best. In conclusion, distinct phenotypic subtypes were identified in community‐dwelling patients with SDB. Tailored strategies to encourage medical care‐seeking, early identification, and optimisation of treatment are necessary considering the different subtypes.
Rapid sleep education: If you could tell people one thing about sleep, what should it be?
Authors: Barley Blake K. , Walter Charles , Orselli Paul , Scullin Michael K. 
Published: November 2022

SummaryInformal learning settings such as museums provide unique opportunities for educating a local community about sleep. However, in such settings, information must be capable of immediately inciting interest. We developed a series of sleep “icebreakers” (brief, informal facts) to determine whether they elicited interest in sleep and encouraged behavioural change. There were 859 participants across three cross‐sectional samples: (a) members of the local museum; (b) Mechanical Turk workers who responded to a “sleep” study advertisement; and (c) Mechanical Turk workers who responded to a “various topics” study advertisement that did not mention sleep. All three samples demonstrated high interest in sleep topics, though delayed recall of the icebreakers was strongest in participants who expected to learn about the sleep topics. Icebreaker interest ratings were independent of age, gender and race/ethnicity, suggesting that sleep is a topic of universal interest. Importantly, regardless of demographics and sample, the more the icebreakers interested the participants, the more likely participants were to indicate willingness to donate to a sleep exhibit, change their sleep behaviours, and post to social media. Thus, sleep icebreakers can rapidly elicit people's interest, and future outreach efforts should couple icebreakers with opportunities for subsequent personalized learning.
Disease burden in primary sclerosing cholangitis in the Netherlands: A long‐term follow‐up study
Authors: van Munster Kim N. , Mol Bregje , Goet Jorn C. , van Munster Sanne N. , Weersma Rinse K. , de Vries Annemarie C. , van der Meer Adriaan J. , Inderson Akin , Drenth Joost P. , van Erpecum Karel J. , Boonstra Kirsten , Beuers Ulrich , Dijkgraaf Marcel G. W. , Ponsioen Cyriel Y. ,   the EpiPSC2 Study Group
Published: November 2022

AbstractBackground & AimsPrimary sclerosing cholangitis (PSC) is a progressive, cholestatic liver disease which greatly impacts the lives of individuals. Burden of disease due to shortened life expectancy and impaired quality of life is ill‐described. The aim of this study was to assess long‐term disease burden in a large population‐based registry with regard to survival, clinical course, quality adjusted life years (QALYs), medical consumption and work productivity loss.MethodsAll PSC patients living in a geographically defined area covering ~50% of the Netherlands were included, together with patients from the three liver transplant centres. Survival was estimated by competing risk analysis. Proportional shortfall of QALYs during disease course was measured relative to a matched reference cohort using validated questionnaires. Work productivity loss and medical consumption were evaluated over time.ResultsA total of 1208 patients were included with a median follow‐up of 11.2 year. Median liver transplant‐free survival was 21.0 years. Proportional shortfall of QALYs increased to 48% >25 years after diagnosis. Patients had on average 12.4 hospital contact days among which 3.17 admission days per year, annual medical costs were €12 169 and mean work productivity loss was 25%.ConclusionsOur data quantify for the first time disease burden in terms of QALYs lost, clinical events, medical consumption, costs as well as work productivity loss, and show that all these are substantial and increase over time.
Genomic and phenotypic changes associated with alterations of migratory behaviour in a songbird
Authors: Zhang Dezhi , She Huishang , Rheindt Frank E. , Wu Lei , Wang Huan , Zhang Kai , Cheng Yalin , Song Gang , Jia Chenxi , Qu Yanhua , Olsson Urban , Alström Per , Lei Fumin 
Published: November 2022

AbstractThe seasonal migration of birds is a fascinating natural wonder. Avian migratory behaviour changes are common and are probably a polygenic process as avian migration is governed by multiple correlated components with a variable genetic basis. However, the genetic and phenotypic changes involving migration changes are poorly studied. Using one annotated near‐chromosomal level de novo genome assembly, 50 resequenced genomes, hundreds of morphometric data and species distribution information, we investigated population structure and genomic and phenotypic differences associated with differences in migratory behaviour in a songbird species, Yellow‐throated BuntingEmberiza elegans(Aves: Emberizidae). Population genomic analyses reveal extensive gene flow between the southern resident and the northern migratory populations of this species. The hand‐wing index is significantly lower in the resident populations than in the migratory populations, indicating reduced flight efficiency of the resident populations. Here, we discuss the possibility that nonmigratory populations may have originated from migratory populations though migration loss. We further infer that the alterations of genes related to energy metabolism, nervous system and circadian rhythm may have played major roles in regulating migration change. Our study sheds light on phenotypic and polygenic changes involving migration change.
Insights into symbiotic interactions from metatranscriptome analysis of deep‐sea mussel Gigantidas platifrons under long‐term laboratory maintenance
Authors: Sun Yan , Wang Minxiao , Chen Hao , Wang Hao , Zhong Zhaoshan , Zhou Li , Fu Lulu , Li Chaolun , Sun Song 
Published: November 2022

AbstractSymbioses between invertebrates and chemosynthetic bacteria are of fundamental importance in deep‐sea ecosystems, but the mechanisms that enable their symbiont associations are still largely undescribed, owing to the culturable difficulties of deep‐sea lives. Bathymodiolinae mussels are remarkable in their ability to overcome decompression and can be maintained successfully for an extended period under atmospheric pressure, thus providing a model for investigating the molecular basis of symbiotic interactions. Herein, we conducted metatranscriptome sequencing and gene co‐expression network analysis of Gigantidas platifrons under laboratory maintenance with gradual loss of symbionts. The results revealed that one‐day short‐term maintenance triggered global transcriptional perturbation in symbionts, but little gene expression changes in mussel hosts, which were mainly involved in responses to environmental changes. Long‐term maintenance with depleted symbionts induced a metabolic shift in the mussel host. The most notable changes were the suppression of sterol biosynthesis and the complementary activation of terpenoid backbone synthesis in response to the reduction of bacteria‐derived terpenoid sources. In addition, we detected the upregulation of host proteasomes responsible for amino acid deprivation caused by symbiont depletion. Additionally, a significant correlation between host microtubule motor activity and symbiont abundance was revealed, suggesting the possible function of microtubule‐based intracellular trafficking in the nutritional interaction of symbiosis. Overall, by analyzing the dynamic transcriptomic changes during the loss of symbionts, our study highlights the nutritional importance of symbionts in supplementing terpenoid compounds and essential amino acids and provides insight into the molecular mechanisms and strategies underlying the symbiotic interactions in deep‐sea ecosystems.