bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frailty as a Priority-Setting Criterion for Potentially Lifesaving Treatment—Self-Fulfilling Prophecy, Circularity, and Indirect Discrimination?
Authors: Holm Søren , Warrington Daniel Joseph 
Published: November 2022

AbstractFrailty is a state of increased vulnerability to poor resolution of homeostasis after a stressor event. Frailty is most frequently assessed in the old using the Clinical Frailty Scale (CSF) which ranks frailty from 1 to 9. This assessment typically takes less than one minute and is not validated in patients with learning difficulties or those under 65 years old. The National Institute for Health and Care Excellence (NICE) developed guidelines that use “frailty” as one of the priority-setting criteria for how scarce, but potentially lifesaving, health care resources should be allocated during the COVID-19 pandemic. Similar guidelines have been developed elsewhere. This paper discusses the ethical implications of such rationing and argues that this is an unproven and ethically problematic form of health care rationing. It specifically discusses: (1) how the frailty ascription becomes a self-fulfilling prophecy, (2) the problematic use of “frailty” in COVID-19 “triage,” (3) the circularity of the link between age and frailty, (4) indirect discrimination because of the use of a seemingly neutral criterion in health care rationing, and (5) the difficult link between comorbidities and frailty. It is found that there was no research into the use of global frailty scores as a criterion for access to acute treatment before January 2020 and so it is concerning how readily frailty scoring has been adopted to ration access to potentially lifesaving treatments. Existing gerontological frailty scoring systems have not been developed for this purpose, and repurposing them creates significant ethical issues.
Infective endocarditis in childhood: a single-centre experience of 26 years
Authors: Demir Fadli , Varan Celal , Erdem Sevcan , Atmış Anıl , Akbaş Tolga , Subaşı Berivan , Güzel Yasin , Özbarlas Nazan 
Published: November 2022

AbstractThe aim of this study was to present the clinical and microbiological characteristics of patients with infective endocarditis.A retrospective evaluation was made of patients diagnosed with infective endocarditis between 1995 and 2021. The clinical and laboratory characteristics of the patients were recorded together with conditions constituting a risk for the development of endocarditis, treatment, and surgical outcomes.Evaluation was made of 68 patients with a mean age of 7.3 years (3 months–17 years), diagnosed with infective endocarditis. An underlying cause of CHD was determined in 47 (69%) patients and rheumatic valve disease in 3 (4.4%). There was no structural heart disease in 18 (26%) patients of whom 13 of them had other risk factors. A causative organism was found in 41 (60%) cases, and the microorganism most often determined was viridans group streptococcus. No difference was determined between the patients diagnosed before and after 2007 in respect of the frequency of viridans streptococcus (p > 0.05). Septic emboli were seen in 18 (26%) patients, of which 17 required surgical treatments. In 5 of 11 (16.6%) patients with mortality, the agent was S. aureus. Of the total 28 (41%) patients were evaluated as hospital-acquired endocarditis. The most frequently determined agents in this group were staphylococcus epidermidis and S. aureus.Although CHDs continue to be the greatest risk factor for endocarditis, there is an increasing frequency of endocarditis in patients with no structural heart disease. Mortality rates are still high in infective endocarditis, especially in S. aureus endocarditis.
Achieving High-Resolution Hard X-ray Microscopy using Monolithic 2D Multilayer Laue Lenses
Authors: Xu Wei , Xu Weihe , Bouet Nathalie , Zhou Juan , Yan Hanfei , Huang Xiaojing , Lu Ming , Zalalutdinov Maxim , Chu Yong S , Nazaretski Evgeny 
Published: November 2022

Abstract:
This article introduces the 2D multilayer Laue lens (MLL) nanofocusing optics recently developed for high-resolution hard X-ray microscopy. The new optics utilized a micro-electro-mechanical-system (MEMS)-based template to accommodate two linear MLL optics in a pre-aligned configuration. Angular misalignment between the two lenses was controlled in tens of millidegrees, and the lateral position error was on a micrometer scale. Using the developed 2D MLLs, an astigmatism-free point focus of approximately 14 nm by 13 nm in horizontal and vertical directions, respectively, at 13.6 keV photon energy was obtained. The success of 2D MLL optics with an approaching 10 nm resolution is a significant step forward for the development of high-resolution hard X-ray microscopy and applications of MLL optics in the hard X-ray community.
A Roadmap for Edge Computing Enabled Automated Multidimensional Transmission Electron Microscopy
Authors: Mukherjee Debangshu , Roccapriore Kevin M , Al-Najjar Anees , Ghosh Ayana , Hinkle Jacob D , Lupini Andrew R , Vasudevan Rama K , Kalinin Sergei V , Ovchinnikova Olga S , Ziatdinov Maxim A , Rao Nageswara S 
Published: November 2022

Abstract:
The advent of modern, high-speed electron detectors has made the collection of multidimensional hyperspectral transmission electron microscopy datasets, such as 4D-STEM, a routine. However, many microscopists find such experiments daunting since analysis, collection, long-term storage, and networking of such datasets remain challenging. Some common issues are their large and unwieldy size that often are several gigabytes, non-standardized data analysis routines, and a lack of clarity about the computing and network resources needed to utilize the electron microscope. The existing computing and networking bottlenecks introduce significant penalties in each step of these experiments, and thus, real-time analysis-driven automated experimentation for multidimensional TEM is challenging. One solution is to integrate microscopy with edge computing, where moderately powerful computational hardware performs the preliminary analysis before handing off the heavier computation to high-performance computing (HPC) systems. Here we trace the roots of computation in modern electron microscopy, demonstrate deep learning experiments running on an edge system, and discuss the networking requirements for tying together microscopes, edge computers, and HPC systems.
GENERALIZED PARTIAL MEET AND KERNEL CONTRACTIONS
Authors: GARAPA MARCO , REIS MAURÍCIO D. L. 
Published: November 2022

AbstractTwo of the most well-known belief contraction operators are partial meet contractions (PMCs) and kernel contractions (KCs). In this paper we propose two new classes of contraction operators, namely the class of generalized partial meet contractions (GPMC) and the class of generalized kernel contractions (GKC), which strictly contain the classes of PMCs and of KCs, respectively. We identify some extra conditions that can be added to the definitions of GPMCs and of GKCs, which give rise to some interesting subclasses of those classes of functions, namely the classes of extensional and of uniform GPMCs/GKCs. In the context of contractions on belief sets the classes of partial meet contractions, uniform GPMCs and extensional GPMCs are all identical. Nevertheless, when considered as operations on belief bases, the class of uniform GPMCs coincides with the class of partial meet contractions, but the extensional GPMCs constitute a new kind of belief base contraction functions whose characterizing postulate of irrelevance of syntax is extensionality—the same postulate of irrelevance of syntax which occurs in the classical axiomatic characterization of partial meet contractions for belief sets—rather than the postulate of uniformity—which is the irrelevance of syntax postulate used in the axiomatic characterization for partial meet contractions on belief bases. Analogous results are obtained regarding the classes of extensional and of uniform GKCs. We present the interrelations in the sense of inclusion among all the new classes of operators presented in this paper and several well known classes of PMCs and of KCs.
Future Changes in Global Runoff and Runoff Coefficient From CMIP6 Multi‐Model Simulation Under SSP1‐2.6 and SSP5‐8.5 Scenarios
Authors: Wang Aihui , Miao Yue , Kong Xianghui , Wu Huan 
Published: November 2022

AbstractThis paper assesses the performances of runoff (Ro) and runoff coefficient (α, the ratio of runoff to precipitation) simulations from 23 models during the historical period and then projects their future changes under the two emission scenarios (SSP1‐2.6 and SSP5‐8.5) in the Coupled Model Intercomparison Project. Compared with the UNH/GRDC Ro dataset (0.82 mm day−1), the multi‐model median (MME) Ro of 1995–2014 produces a comparable global mean magnitude (0.80 mm day−1), displays a similar spatial distribution of mean Ro, and also well captures the seasonal cycles at both global and basin scales. The global mean Ro of MME is projected to be increased by 0.01–0.02 mm day−1 (SSP1‐2.6) and 0.02–0.10 mm day−1 (SSP5‐8.5) during the twenty‐first century. Regional hotspots for strong increasing Ro appear across most areas of northern high latitudes, Africa, and southeastern Asia, with high inter‐model consistency. The global mean α is projected to be slightly decreased (−0.17 to −0.63%) except for the long‐term under the SSP5‐8.5 (0.26%). Although signs of changes in Ro vary with the river basins, periods, and scenarios, α in more than half (7 out of 12) river basins are projected to decrease. The uneven distributions of projected Ro changes over global land areas are related to the response of multiple hydroclimatic variables to the global warming. Given regions with inconstancy change signs of the projected precipitation, we speculate that changes in Ro are affected by more complicated hydroclimatic processes that warrant further investigations with physical‐based approaches.
Multiphysics Modeling of Volcanic Unrest at Mt. Ruapehu (New Zealand)
Authors: Arens Fee , Coco Armando , Gottsmann Joachim , Hickey James , Kilgour Geoff 
Published: November 2022

AbstractPre‐eruptive signals at the crater lake‐bearing Mt. Ruapehu (New Zealand) are either absent or hard to identify. Here, we report on geophysical anomalies arising from hydrothermal unrest (HTU) and magmatic unrest (MU) using multiphysics numerical modeling. Distinct spatio‐temporal anomalies are revealed when jointly solving for ground displacements and changes in gravitational and electrical potential fields for a set of subsurface disturbances including magma recharge and anomalous hydrothermal flow. Protracted hydrothermal injections induce measurable surface displacements (>0.5 cm) at Ruapehu's summit plateau, while magmatic pressurization (5–20 MPa) results in ground displacements below detection limits. Source density changes of 10 kg/m3 (MU simulations) and CO2 fluxes between 2,150 and 3,600 t/d (HTU simulations) induce resolvable residual gravity changes between +8 and −8 μGal at the plateau. Absolute self‐potential (SP) anomalies are predicted to vary between 0.3 and 2.5 mV for all unrest simulations and exceed the detection limit of conventional electric surveying. Parameter space exploration indicates that variations of up to 400% in the Biot‐Willis coefficient produce negligible differences in surface displacement in MU simulations, but strongly impact surface displacement in HTU simulations. Our interpretation of the findings is that monitoring of changes in SP and gravity should permit insights into MU at Ruapehu, while HTU is best characterized using ground displacements, residual gravity changes and SP anomalies. Our findings are useful to inform multiparameter monitoring strategies at Ruapehu and other volcanoes hosting crater lakes.
Survival of compromised adult sensory neurons involves macrovesicular formation
Authors: Krishnan Anand , Areti Aparna , Komirishetty Prashanth , Chandrasekhar Ambika , Cheng Chu , Zochodne Douglas W. 
Published: November 2022

AbstractAdult neurons are recognized as post-mitotically arrested cells with limited regenerative potential. Given these restraints, it is perplexing how neurons sustain routine physiological and occasional reparative stress without compromising their density and integrity. We observed that specific insults or physiological alterations drive adult sensory neurons to attempt cell cycle entry. In this context, we demonstrate that at least a small population of sensory neurons modify their cytoskeleton as a survival mechanism in settings of growth arrest and associated stress. Most notably, among their apparent survival modifications is included a unique, and uncharacterized form of macrovesicle shedding and a subsequent neuron size adjustment. Using time-lapse imaging, we demonstrate macrovesicle shedding in some neurons subjected to growth restraint, but not associated with apoptosis. In axotomized neurons in vivo, cell cycle entry was rare to absent and macrovesicles were not observed, but we nonetheless identified changes in mRNA associated with autophagy. In vivo, neighbouring macrophages may have a role in modifying the neuron cytoskeleton after axotomy. Overall, the findings identify previously unrecognized structural adaptations in adult sensory neurons that may provide resilience to diverse insults.
Inference of epigenetic subnetworks by Bayesian regression with the incorporation of prior information
Authors: Jing Anqi , Han Jie 
Published: November 2022

AbstractChanges in gene expression have been thought to play a crucial role in various types of cancer. With the advance of high-throughput experimental techniques, many genome-wide studies are underway to analyze underlying mechanisms that may drive the changes in gene expression. It has been observed that the change could arise from altered DNA methylation. However, the knowledge about the degree to which epigenetic changes might cause differences in gene expression in cancer is currently lacking. By considering the change of gene expression as the response of altered DNA methylation, we introduce a novel analytical framework to identify epigenetic subnetworks in which the methylation status of a set of highly correlated genes is predictive of a set of gene expression. By detecting highly correlated modules as representatives of the regulatory scenario underling the gene expression and DNA methylation, the dependency between DNA methylation and gene expression is explored by a Bayesian regression model with the incorporation of g-prior followed by a strategy of an optimal predictor subset selection. The subsequent network analysis indicates that the detected epigenetic subnetworks are highly biologically relevant and contain many verified epigenetic causal mechanisms. Moreover, a survival analysis indicates that they might be effective prognostic factors associated with patient survival time.
Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids
Authors: Seiler Spencer T. , Mantalas Gary L. , Selberg John , Cordero Sergio , Torres-Montoya Sebastian , Baudin Pierre V. , Ly Victoria T. , Amend Finn , Tran Liam , Hoffman Ryan N. , Rolandi Marco , Green Richard E. , Haussler David , Salama Sofie R. , Teodorescu Mircea 
Published: November 2022

AbstractOrgan-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology and physiology of their in vivo counterparts. Here, we have developed a multiplex platform that automates the culture of individual organoids in isolated microenvironments at user-defined media flow rates. Programmable workflows allow the use of multiple reagent reservoirs that may be applied to direct differentiation, study temporal variables, and grow cultures long term. Novel techniques in polydimethylsiloxane (PDMS) chip fabrication are described here that enable features on the upper and lower planes of a single PDMS substrate. RNA sequencing (RNA-seq) analysis of automated cerebral cortex organoid cultures shows benefits in reducing glycolytic and endoplasmic reticulum stress compared to conventional in vitro cell cultures.
Efficacy of plasma activated saline in a co-culture infection control model
Authors: Tsoukou Evanthia , Bourke Paula , Boehm Daniela 
Published: November 2022

AbstractPlasma activated liquids have demonstrated antimicrobial effects and receive increasing attention due to the potential to strengthen the armoury of novel approaches against antibiotic resistant bacteria. However, the antibacterial activity and cytotoxic effects of these solutions need to be understood and balanced before exposure to humans. In this study, the antibacterial effects of plasma activated saline (PAS) were tested against Gram negative and positive bacteria, and HaCaT keratinocytes were used for cytotoxicity studies. For the first time, a co-culture model between these bacteria and eukaryotic cells under the influence of PAS has been described. Exposure of saline to plasma resulted in high concentrations of nitrate, hydrogen peroxide and a reduction of pH. PAS caused high antibacterial effects in the co-culture model, accompanied by high cytotoxic effects to the monolayer of mammalian cells. We present evidence and provide a deeper understanding for the hypothesis that upon treatment with PAS, chemical species generated in the liquid mediate high antimicrobial effects in the co-culture setup as well as mitochondrial depolarization and glutathione depletion in HaCaT cells and cell lysis due to acidic pH. In conclusion, PAS retains strong antibacterial effects in a co-culture model, which may have unintended negative biological effects on mammalian cells.
Taphonomic and technological analyses of Lower Palaeolithic bone tools from Clacton-on-Sea, UK
Authors: Parfitt Simon A. , Lewis Mark D. , Bello Silvia M. 
Published: November 2022

AbstractThe exceptional survival of Middle Pleistocene wooden spears at Schöningen (Germany) and Clacton-on-Sea (UK) provides tantalizing evidence for the widespread use of organic raw materials by early humans. At Clacton, less well-known organic artefacts include modified bones that were identified by the Abbé Henri Breuil in the 1920s. Some of these pieces were described and figured by Hazzledine Warren in his classic 1951 paper on the flint industry from the Clacton Channel, but they have been either overlooked in subsequent studies or dismissed as the product of natural damage. We provide the first detailed analysis of two Clactonian bone tools found by Warren and a previously unrecognized example recovered in 1934 during excavations directed by Mary Leakey. Microscopic examination of percussion damage suggests the bones were used as knapping hammers to shape or resharpen flake tools. Early Palaeolithic bone tools are exceedingly rare, and the Clacton examples are the earliest known organic knapping hammers associated with a core-and-flake (Mode 1) lithic technology. The use of soft hammers for knapping challenges the consensus that Clactonian flintknapping was undertaken solely with hard hammerstones, thus removing a major technological and behavioural difference used to distinguish the Clactonian from late Acheulean handaxe (Mode 2) industries.
Gender differences in the prevalence and impact factors of adolescent dissociative symptoms during the coronavirus disease 2019 pandemic
Authors: Cheng Qinglin , Zhao Gang , Chen Junfang , Deng Yuanyuan , Xie Li , Wang Le 
Published: November 2022

AbstractThe purpose of this study was to explore the differences between the prevalence and impact factors of adolescent dissociative symptoms (ADSs) by using sex-stratification during the coronavirus disease 2019 (COVID-19) pandemic. A school-based, two-center cross-sectional study was conducted in Hangzhou City, China, between January 1, 2021 and April 30, 2022. The sample included 1,916 adolescents aged 13–18 years that were randomly selected using a multiphase, stratified, cluster sampling technique. A two-stage assessment procedure was used to find out the ADSs. We used a multivariate logistic regression analysis to assess the impact factors of ADSs during the COVID-19 pandemic. The adolescent dissociative scores (t = 4.88, P < 0.001) and positive ADSs rate (Chi-square = 15.76, P < 0.001) in males were higher than in females. Gender-stratified, stepwise multiple logistic regression analysis revealed that the conflict relationship of teacher-student [adjusted odds ratio (AOR) 1.06, 95% confidence interval (CI) 1.01–1.10], family expressiveness (AOR 0.87, 95% CI 0.78–0.98), family conflict (AOR 1.15, 95% CI 1.05–1.27), family organization (AOR 0.88, 95% CI 0.78–0.99), and family cohesion (AOR 0.87, 95% CI 0.77–0.99) were linked to ADSs only in males, while individual psychological states of somatic complaint (AOR 1.04, 95% CI 1.00–1.08) and paranoid ideation (AOR 1.09, 95% CI 1.01–1.19) were associated with female ADSs only. The ADSs seemed to be prevalent in Hangzhou City, studied during the COVID-19 pandemic. Gender differences in the prevalence and impact factors of dissociative symptoms seem to be significant among adolescents. Thus, gender-specific intervention programs against ADSs should be considered as reducing this risk.
Reduction of hexavalent chromium using bacterial isolates and a microbial community enriched from tannery effluent
Authors: Plestenjak Eva , Kraigher Barbara , Leskovec Simona , Mandic Mulec Ines , Marković Stefan , Ščančar Janez , Milačič Radmila 
Published: November 2022

AbstractWe investigated microbial growth in increasing concentrations of hexavalent chromium (Cr(VI)) and its reduction by a microbial community enriched from tannery effluent and by the bacterial strains isolated from the enriched community. The bacterial growth was monitored by measuring the optical cell density (OD650), while the Cr(VI) concentration in the samples was determined using spectrophotometry and liquid chromatography hyphenated to inductively coupled plasma mass spectrometry (HPLC–ICP–MS). At a Cr(VI) concentration of 100 mg/L, the isolates affiliated with Pseudomonas aeruginosa (P. aeruginosa) reached higher optical cell densities, but were in general less effective for Cr(VI) reduction than the isolates affiliated with Mammaliicoccus sciuri (M. sciuri). All three M. sciuri isolates and only one of the seven P. aeruginosa isolates were able to reduce 50% of the Cr(VI) with an initial concentration of 100 mg/L within 24 h (pH 7.1), while the six isolates affiliated with P. aeruginosa were less effective. Compared to the isolated, individual bacterial strains, the enriched microbial community was better adapted to the elevated Cr(VI) concentrations, but needed a longer time (48 h) to reduce the Cr(VI) with the same efficacy as the most efficient individual isolates. The ability of the enriched microbial community and the isolated bacterial strains to reduce the Cr(VI) highlights their potential for use in the rapid bioremediation of wastewaters contaminated with Cr(VI).