bluuebunny/crossref_metadata_2025_split huggingface dataset:
Employment risks of all employed working-age cancer survivors after cancer diagnosis: a retrospective cohort analysis of population-based administrative data
Authors: Choi Yoon-Joo , Lee Weon-Young 
Published: August 2022

Abstract
PURPOSE : Discussions on the employment status of cancer survivors should be actively examined worldwide as the cancer survival rate increases. This study attempted to examine the status of working status changes after cancer diagnosis to improve the quality of life of cancer survivors.METHODS: We established a nationwide cohort to determine long-term changes of work after cancer diagnosis. All patients aged 19 to 50 years who were newly diagnosed with cancer while working for the previous 3 years were considered as the case group in 2010. Using propensity score matching (PSM), the cases were matched by sex and age at a ratio 1:3 with the control group. The Kaplan-Meier curve and Cox hazard model analyses were performed to determine the occurrence of unemployment and return to work in the case and control groups on a yearly basis.RESULTS: According to the 6-year follow-up after cancer diagnosis, 26.5% of cancer patients and 23.2% of controls had lost their jobs by the end of the follow-up (P<.0001). Meanwhile, 50.5% of cancer patients and 57.4% of control patients returned to work after unemployment (P<.0001). Subsequently, based on the Cox hazard model, the unemployment risk of cancer patients was 1.42 times higher while the proportion of return to work was 1.15 times lower than those of the general population.CONCLUSIONS: Employment is very closely related to the quality of life of cancer survivors. These results highlight that there is a need for a system that can support cancer survivors' work maintenance and return to work after unemployment during the treatment period, and that awareness of this needs to be improved.
Autoimmune Gene Expression Profiling of Fingerstick Whole Blood in Chronic Fatigue Syndrome
Authors: Wang Zheng , Waldman Michelle F. , Basavanhally Tara J. , Jacobs Aviva R. , Lopez Gonzalo , Perichon Regis Y. , Ma Johnny J. , Mackenzie Elyse M. , Healy James , Wang Yixin , Hersey Sarah A. 
Published: August 2022

Abstract
Background: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating condition that can lead to severe impairment of physical, psychological, cognitive, social, and occupational functions. The cause of ME/CFS remains incompletely understood. There is no clinical diagnostic test for ME/CFS. Although many therapies have been used off-label to manage symptoms of ME/CFS, there are limited, if any, specific therapies or cure for ME/CFS. In this study, we investigated the expression of genes specific to key immune functions, and viral infection status in ME/CFS patients with an aim of identifying biomarkers for characterization and/or treatment of the disease. Methods In 2021, one-hundred and sixty-six (166) patients diagnosed with ME/CFS and 83 healthy controls in the US participated in this study via a social media-based application (app). The patients and heathy volunteers consented to the study and provided self-collected finger-stick blood and first morning void urine samples from home. RNA from the fingerstick blood was tested using DxTerity’s 51-gene autoimmune RNA expression panel (AIP). In addition, DNA from the same fingerstick blood sample was extracted to detect viral load of 4 known ME/CFS associated viruses (HHV6, HHV7, CMV and EBV) using a real-time PCR method. Results Among the 166 ME/CFS participants in the study, approximately half (49%) of the ME/CFS patients reported being house-bound or bedridden due to severe symptoms of the disease. From the AIP testing, ME/CFS patients with severe, bedridden conditions displayed significant increases in gene expression of IKZF2, IKZF3, HSPA8, BACH2, ABCE1 and CD3D, as compared to patients with mild to moderate disease conditions. These six aforementioned genes were further upregulated in the 22 bedridden participants who suffer not only from ME/CFS but also from other autoimmune diseases. These genes are involved in T cell, B cell and autoimmunity functions. Furthermore, IKZF3 (Aiolos) and IKZF2 (Helios), and BACH2 have been implicated in other autoimmune diseases such as systemic lupus erythematosus (SLE) and Rheumatoid Arthritis (RA). Among the 240 participants tested with the viral assays, 9 samples showed positive results (including 1 EBV positive and 8 HHV6 positives). Conclusions Our study indicates that gene expression biomarkers may be used in identifying or differentiating subsets of ME/CFS patients having different levels of disease severity. These gene targets may also represent opportunities for new therapeutic modalities for the treatment of ME/CFS. The use of social media engaged patient recruitment and at-home sample collection represents a novel approach for conducting clinical research which saves cost, time and eliminates travel for office visits.
Correlation between intracerebral pH and expression of Hv1 proton channel and myelin-related proteins after hypoxic-ischemic brain injury in newborn pigs
Authors: Zheng Yang , Wang Xiaoming 
Published: August 2022

AbstractBackground Myelin sheath injury after cerebral hypoxia-ischemia is well known and is associated with multiple factors. In this study, changes in pH and expression of voltage-gated proton channel (Hv1) as well as myelin-related proteins in the brain, after hypoxic-ischemic brain injury (HIBI) in neonatal pigs were analyzed. Methods A total of 53 healthy male neonatal pigs were used to establish an HIBI model. The control group was without HI process. The model group underwent hypoxic-ischemic process for 40 min. The pH and expression of Hv1, myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG), and myelin-associated glycoprotein (MAG) in the brain tissue of neonatal pigs were then analyzed at different time points after HI. Results After HIBI, the expression of Hv1 expression reached a peak at 2–6 h. The pH in this brain region reached a minimum at 0–2 h after HI, and was then gradually restored. Regarding the expression of myelin-related proteins, we observed an initial decrease in their levels, followed by an increase. MAG and MBP expression reached a minimum at 6–12 h, while MOG expression reached a minimum at 2–6 h. Furthermore, a correlation was found between pH and Hv1, MBP, and MOG expression, but not with MAG expression. Conclusions Our findings indicate that brain pH is correlated with Hv1, MBP, and MOG expression after HIBI, suggesting that changes in the brain environment impact myelin damage and repair to some extent.
Development of a Mobile-accessible Web Application in Wound Management: A Pilot Study
Authors: Yılmaz Ayşe Çelik , Bayraktar Abdullah Salih , Özbek Uğur , Çeken Celal , Açıl Hande Cengiz , Erkin Alper , Aygin Dilek 
Published: August 2022

Abstract
Introduction and purpose: With the development of technology, new applications have started to deliver health services. These applications, which are described as mobile health applications, provide a systematic approach to managing chronic wounds and increase patients' quality of life and their compliance with treatment. In this direction, the study was carried out to develop an application that can be used by patients and healthcare professionals in chronic wound management.Material and Method: To develop the application, first of all, the user panels and their contents were drafted, and the servers and infrastructure works were started. Interfaces were developed based on the page designs created, and separate pages were designed for patients and healthcare personnel. After the development of the main operation and management platform, the definition of services, the editing of image processing features, and the security tests, the mobile application was made ready for use. In the pilot study, eight patients with diabetic foot wounds were followed up with the application used on Android systems via web browsers, both tablets, and smartphones, via mobile application.Results: In the study, it was determined that both the patients followed by the wound tracking system and the health personnel using the system were satisfied with the application.Conclusion: Using mobile applications in the long-term treatment process of chronic wounds increases the patients' follow-up outside the hospital, their compliance with the treatment, and their comfort. In terms of health personnel, it also reduces the intensity and workload in the hospital and ensures that patient records are kept regularly. Patients with chronic wounds with no indication for hospitalization can be followed up with the mobile application.
Regulation of pseurotin A biosynthesis by GliZ and the synergetic effects of gliotoxin and pseurotin A biosynthesis on virulence of Aspergillus fumigatus
Authors: Seo Hyewon , Kang Suzie , Lee Dongho , Yun Cheol-Won 
Published: August 2022

Abstract
Recently, we reported that zinc regulates gliotoxin biosynthesis via ZafA, which is a zinc-responsive transcriptional activator. From an HPLC analysis of culture media of Aspergillus fumigatus, we found a trend of decreasing gliotoxin production but increasing pseurotin A and fumagillin production in proportion to the zinc concentration. The expression of the genes involved in pseurotin A biosynthesis was upregulated under high zinc concentrations. Furthermore, upregulated expression of pseurotin A biosynthetic genes and higher production of pseurotin A were observed in the ZafA deletion strain. Interestingly, the deletion of GliZ, a transcriptional activator of gliotoxin biosynthesis genes, resulted in upregulated expression of pseurotin A biosynthetic genes and increased production of pseurotin A. We detected upregulation of FumR expression in the GliZ and ZafA deletion mutants. The overexpression of GliZ observed in the ZafA deletion mutant resulted in the failure of the mutant to increase pseurotin A production, which is a phenotype of the ZafA deletion mutant. These results suggest that ZafA sequentially regulates pseurotin A biosynthesis through GliZ. Finally, we found through a murine virulence test that the GliZ and FumR double-deletion mutants showed a delayed death rate compared with the single-deletion mutants of either GliZ or FumR. Taken together, these results suggested that the biosyntheses of gliotoxin and pseurotin A are regulated in opposite ways by zinc utilization and that each secondary metabolite is synthesized when the synthesis of another secondary metabolite fails to protect it against the defense system of the host.
Cabozantinib-induced serum creatine kinase elevation and rhabdomyolysis: a retrospective study
Authors: Yamanaka Taro , Takemura Kohji , Hayashida Michikata , Suyama Koichi , Urakami Shinji , Miura Yuji 
Published: August 2022

Abstract
BackgroundRhabdomyolysis is a potentially fatal disease, and elevated serum creatine kinase (CK) is one of the key laboratory findings suggestive of this disease. It is important to distinguish rhabdomyolysis from other diseases because CK elevation can occur in a variety of etiologies. Cabozantinib is one of the standard treatments for patients with renal cell carcinoma, but the frequency of CK elevation with this drug is still unknown.MethodsTo investigate the frequency of serum CK elevation induced by cabozantinib, we retrospectively reviewed the electronic medical records of patients with advanced renal cell carcinoma who received cabozantinib at our institution from April 2020 to March 2021.ResultsSeven patients were included in the study; six experienced serum CK elevation, four were classified as grade 1, and the remaining two as grade 3 according to the Common Terminology Criteria for Adverse Events (CTCAE) version 5.0. In five patients, the time from cabozantinib administration to CK elevation was 14 days. Hypothyroidism can be one of the causes of CK elevation; however, two patients who had hypothyroidism caused by previous treatment were in the euthyroid condition due to hormone replacement. One patient with grade 1 CK elevation developed late-onset immune-related adverse event (irAE) myositis comorbid with suspected myasthenia gravis. One patient with grade 3 CK elevation developed muscle weakness and rhabdomyolysis.ConclusionsCK elevation is a common adverse event induced by cabozantinib. Most patients are asymptomatic and may not be clinically ill. However, physicians should be careful with the rare occurrence of symptomatic serum CK elevation, indicating rhabdomyolysis and neuromuscular irAEs, especially in patients who receive immune checkpoint inhibitors (ICIs).
Current knowledge and attitudes of students towards biotechnology: A long way to go
Authors: Alberro Ainhoa , Ortega-Lasuen Unai , Diez José Ramón 
Published: August 2022

Abstract
Thanks to technological innovations, science is rapidly evolving, and therefore, development of effective and engaging learning strategies is needed to ensure knowledge transmission. But first, we need to know what the current situation is. In this work, we focused on biotechnology, a scientific branch that is present in many of aspects of our daily life, but also a source of controversy. We described the knowledge of Baccalaureate students about biotechnology and its uses, as well as their awareness about the transgenic food as a socioscientific issue, and their attitudes towards applications of transgenics. Most students cannot give an appropriate definition of biotechnology. Besides, students have misconceptions about transgenics, and easily change their attitudes. Thus, new strategies to get students interest and improve knowledge transfer should be implemented.
Influence of childhood maltreatment on major depressive disorder in adulthood: mediating role of social support
Authors: Zeng Zhi , Lv Xiaozhen , Zhou Shuzhe , Liu Qi , Si Tianmei , Zhu Gang , Chen Qiaoling , Tian Hongjun , Zhang Nan , Zhang Kerang , Wang Xueyi , Wei Jing , Wang Gang , Yu Xin 
Published: August 2022

Abstract
Background: Childhood maltreatment is strongly associated with major depressive disorder (MDD) in adulthood, social support can buffer the effect of childhood maltreatment on MDD, but there are few studies on the role of different types of social support and gender differences in mediating models.Methods: The study included 965 MDD patients and 443 healthy participants from nine centers. MDD patients are diagnosed using the Diagnostic and Statistical Manual of Mental Disorders-Fourth Edition (DSM-IV). The Childhood Trauma Questionnaire-Short Form (CTQ-SF), 17-item Hamilton Depression Rating Scale and Social Support Rate Scale (SSRS) were used to assess the status of childhood maltreatment, MDD and social support. Multivariable mediation analyses were used to examine the mediating role of social support between childhood maltreatment and MDD.Results: After controlling for sex, age, ethnicity, educational level, family history, work status and marriage, childhood maltreatment increases the risk of MDD (OR=1.07, 95%CI: 1.05-1.09). Total social support (OR=0.88, 95%CI: 0.86-0.90) , subjective social support (OR=0.83, 95%CI：0.80-0.86), objective social support (OR=0.84, 95%CI: 0.80-0.89) and support utilization (OR=0.73, 95%CI: 0.68-0.78) all significantly reduced the risk of MDD. Total social support, subjective support and support utilization played a mediating role in childhood maltreatment and MDD, but not objective social support. For males, only subjective social support mediated the relationship between childhood maltreatment and MDD, while for females, both subjective social support and support utilization mediated the relationship.Conclusions: For those who experienced childhood maltreatment, suitable social support, especially subjective support and support utilization, may reduce the risk of MDD. Different social support seems necessary for male and female for alleviating the influence of childhood maltreatment on MDD.
Silencing of MEG3 gene promoted anti-cancer activity and drug sensitivity in glioma
Authors: Avsar Timucin , Degirmenci Zehra , Kilic Turker 
Published: August 2022

Abstract
Aberrant expression of MEG3 has been shown in various cancers. The purpose of this study is to evaluate the effect of MEG3 on glioma cells including U87MG and patient-derived primary tumors cells and compare with nonglial HUVEC cells. Cell viability, migration and chemo sensitivity was assayed. Apoptosis was evaluated in MEG3 overexpressing and MEG3 suppressed cells. MEG3 expression was compared in patient-derived tumor cells with respect to IDH1 mutation status and WHO-grades. Silencing of MEG3 inhibited cell proliferation and reduced cell migration while overexpression of MEG3 promoted cell proliferation in glioma cells. MEG3 inhibition improved chemo sensitivity of glioma cells to 5-fluorouracil(5FU) but not to navitoclax. Suppression of MEG3 gene in patient-derived oligodendroglioma cells also showed the same effect whereas glioblastoma cell proliferation and chemo sensitivity were not affected by MEG3 inhibition. Further, cell death mechanism of action was found as apoptosis. Although MEG3 is a widely known tumor suppressor gene and its loss is associated with several cancer types, here we reported that MEG3 inhibition can be used in drug sensitivity for improving efficiency of known chemotherapeutics. We propose that the level of MEG3 should be evaluated in treatment of different glioma subtypes that are resistant to effective drugs.
Development of Ultra High-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Enantiomer Resolution of Thyroxine on a Chiral Crown Ether Derived Chiral Stationary Phase
Authors: Lee Jisun , Adhikari Suraj , Lee Wonjae , Yoon Hye-Ran 
Published: August 2022

Abstract
Chiral discrimination of thyroxine (T4) enantiomers was performed using ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) on a chiral crown ether-derived ChiroSil SCA(-) column. The different composition of mobile phases and the effect of column oven temperatures were investigated and the optimum chromatographic separation with respect to resolution and analysis time was achieved using a mixture of 60% methanol/water (v/v) with 0.1% formic acid at 40°C having a flow rate of 1.4 mL min− 1. The thermodynamic data from van’t Hoff plots of temperature experiments revealed that the enantioseparation was enthalpically favored process. The method was validated in the concentration range of 0.5–100 µg mL− 1 for both enantiomers and proved to be rapid, precise, sensitive, and selective method for the enantiodiscrimination of T4 under the optimized conditions. The calibration curves of both D- and L-T4 showed an excellent linearity with coefficient of determination (R2) > 0.9997. The developed chiral method was successfully applied for a quantitative assay to check the enantiomeric purities of the six pharmaceutical formulations of levothyroxine sodium tablets and the enantiomeric impurities identified were in the range of 0.11–0.29%. This method could be applied for the determination of enantiomeric purity on pharmaceuticals and also for the monitoring of thyroid hormone levels.
Proton Beam Therapy with Pencil Beam Scanning is Safe and Effective for Advanced Hepatocellular Carcinoma and Intrahepatic Cholangiocarcinoma: A Single Center Experience
Authors: Yang Alexander H. , Urrunaga Nathalie H. , Siddiqui Osman , Wu Angela , Schliep Matthew , Molitoris Jason K. , Shetty Kirti , Lominadze Zurabi 
Published: August 2022

Abstract
Background Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA) are the two most common primary liver tumors. Proton Beam Therapy (PBT) is a non-surgical treatment that spares adjacent tissues compared to photon radiation, limiting damage to nearby structures. We present a single center experience on liver toxicity, tumor response, and survival in patients with HCC and iCCA treated with PBT with Pencil Beam Scanning (PBS).MethodsConsecutive patients receiving PBT for HCC or iCCA were reviewed. PBT was delivered with hypofractionated or stereotactic body radiation therapy using PBS. Tumor size was approximated by clinical target volume (CTV). Liver toxicity was determined by changes in MELD-Na and albumin-bilirubin (ALBI) grade. Tumor response was determined by imaging at six to eight weeks post-therapy.Results44 consecutive patients were included, 22 patients in each group. 35% of patients had multifocal disease and median CTV was 232.5cc. Overall survival was 82%. For both cancer types, tumor size, laboratory values, and total radiation dose were not associated with liver toxicity. All iCCA patients had stable or improved ALBI grade following treatment, however there were 4 deaths. Among HCC patients, ALBI grade was stable in 83%, average MELD-Na score remained stable, and there was only one death.ConclusionsOur results indicate that PBT for unresectable HCC and iCCA is safe and effective, even for large and multifocal tumors. Liver function was preserved even in those with baseline cirrhosis who received biologically effective doses ≥80.5 Gy. Overall survival was 86.4%, similar between HCC and iCCA.
Investigation of cryoprotectants -treated protein deterioration during chilled and frozen storage: Electrophoretic pattern, functional properties and kinetic modeling
Authors: Maghsoudi Leila , Moosavi-Nasab Marzieh , Abedi Elahe , Maleki Shahrzad 
Published: August 2022

Abstract
The relative cryoprotective effects of flaxseed protein and pectin in comparison with conventional cryoprotectant on stability of Capoor (Cyprinus carpio) surimi proteins during storage at -20°C for 4 months and at 4°C for 10 days were investigated. The results show that the reaction rate constants of second order kinetic, k, in samples stored at -20°C were much smaller than similar samples stored at 4°C, indicating decreased the rate of protein denaturation in frozen samples. Although, pectin caused to improve water-holding capacity (27.8%; 4°C and 21.5%; -20°C) on account of the formation of a separate gel and represent highly more inhibitory impact on the ice crystals growth, during the overnight soaking, protein denaturation may have been occurred. The flaxseed protein -treated sample showed the lowest decrease of SH content and reaction rate constant at both − 20°C and 4°C than others. The results exhibited that salt extractable protein and sulfhydryl content changes were in good agreement with the second order kinetic model. Regarding to SDS- PAGE results, no major changes were observed in intensity of myosin heavy chain, actin and myosin light chain bands in flaxseed proteins treated surimi over chilled and frozen storage.