bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early Maastrichtian-Early Eocene Biostratigraphy of El Hasana Area, Central Sinai, Egypt
Authors: El Shahawy Adel , Ismail Ahmed , Faris Mahmoud , Naby Ahmed Abdel 
Published: April 2022

ABSTRACTTen calcareous nannofossils and ten planktic foraminiferal biozones were recognized from two sections measured in El Hasana area, in Central Sinai, Egypt. In the West El Hasana section, an early Maastrichtian/early Paleocene (K/Pg) unconformity was recorded due to the absence of the planktic foraminiferal Gansserina gansseri, Abathomphalus mayaroensis, and Globigerina eugubina Zones and the calcareous nannoplankton Micula prinsii and Markalius inversus Zones. The early Paleocene is represented by two biozones, Morozovella pseudobulloides (P1b) and Morozovella trinidadensis (P1c) that correlate with the Cruciplacolithus tenuis Zone (NP2), Chiasmolithus danicus Zone (NP3), and Ellipsolithus macellus Zone (NP4). In both sections, during the middle Paleocene three biozones were recorded: Morozovella uncinata (P2), the lower part of P3 Morozovella angulata, and Planorotalites pusilla pusilla (upper part of P3). The first and second biozones are equated with the upper two thirds of Ellipsolithus macellus (NP4), while the third one is correlated with Fasciculithus tympaniformis Zone (NP5). In the West El Hasana section, the Planorotalites pseudomenardii Zone (P4) ends the measured section. A small hiatus was observed across the late Paleocene/early Eocene (P/E) boundary in the East El Hasana section. This hiatus is due to the absence of the Planorotalites pseudomenardii Zone (P4), Morozovella velascoensis Zone (P5), M. edgari Zone (lower part of P6) and Heliolithus kleinpellii Zone (NP6), Discoaster molheri Zone (NP7), Heliolithus riedelli/Discoaster nobilis Zone (NP8), and Discoaster spp. (e.g., D. araneus) Subzone NP9a. The P/E boundary in East El Hasana section is delineated with the appearance of Rhomboaster spp. (e.g., Rhomboaster bitrifida) NP9b Subzone that coincides with the appearance of the planktic foraminiferal Morozovella subbotinae Zone (upper part of P6).
Chest radiotherapy after left upper lobectomy may be a risk factor for thrombosis in the pulmonary vein stump
Authors: Song Cheng-Yang , Kimura Daisuke , Fukuda Ikuo , Tsushima Fumiyasu , Sakai Takehiro , Tsushima Takao 
Published: April 2022

Abstract
Background: Thrombosis in the pulmonary vein stump (PVS) is not a well-known complication after pulmonary lobectomy, but it has the potential to cause embolism to vital organs. The aim of this study was to evaluate the risk factors for thrombosis in the PVS after pulmonary lobectomy.Methods: A total of 439 patients who underwent pulmonary lobectomy from 2008 to 2017 were retrospectively reviewed, and 412 patients were further analyzed. The state of the PVS was evaluated by chest contrast-enhanced computed tomography (CECT). Univariate analysis was performed to evaluate the potential risk factors for thrombosis in the PVS.Results: Thrombosis in the PVS was detected in 6/412 (1.5%) patients, and 5 of them underwent left upper lobectomy (LUL) (5/100, 5.0%) (P = 0.004). In the analyses of the LUL group, postoperative chest radiotherapy was identified as a risk factor for thrombosis in the PVS (P = 0.024), and postoperative atrial fibrillation (AF) showed a tendency to be a risk factor for thrombosis (p = 0.058).Conclusions: Chest radiotherapy after LUL is a possible risk factor for thrombosis in the PVS. Periodic chest CECT is recommended after postoperative chest radiotherapy for patients after LUL.
Heterozygous expression of the Alzheimer’s disease protective PLCγ2 P522R variant enhances Aβ clearance while preserving synapses.
Authors: Solomon Shiden , Sampathkumar Nirmal Kumar , Carre Ivo , Mondal Mrityunjoy , Chennell George , Vernon Anthony C. , Ruepp Marc-David , Mitchell Jacqueline 
Published: April 2022

Abstract
BACKGROUND: A rare coding variant, P522R, in the phosphoplipase C gamme 2 (PLCG2) gene has been identified as protective against late onset Alzheimer’s disease (AD), but the mechanism is unknown. PLCG2 is exclusively expressed in microglia within the central nervous sytem, and altered microglial function has been implicated in the progression of AD.METHODS: Healthy control hiPSCs were CRISPR edited to generate cells heterozygous and homozygous for the PLCG2P522R variant. Microglia derived from these hiPSC’s were used to investigate the impact of PLCγ2P522R on disease relevant processes, specifically microglial capacity to take up amyloid beta (Aβ) and synapses. Targeted qPCR assessment was conducted to explore expression changes in core AD linked and microglial genes, and mitochondrial function was assessed using an Agilent Seahourse assay.RESULTS Heterozygous expression of the P522R variant resulted in increased microglial clearance of Aβ, while preserving synapses. This was associated with the upregulation of a number of genes, including the anti-inflammatory cytokine Il-10, and the synpase-linked CX3CR1, as well as alterations in mitochondrial function, and increased cellular motility. The protective capacity of PLCγ2P522R appeared crucially dependent on (gene) ‘dose’, as cells homozygous for the variant showed reduced synapse preservation, and a differential gene expression profile relative to heterozygous cells.CONCLUSIONS: These findings suggest that PLCγ2P522R may result in increased surveillance by microglia, and prime them towards an anti-inflammatory state, with an increased capacity to respond to increasing energy demands, but highlights the delicate balance of this system, with increasing PLCγ2P522R ‘dose’ resulting in reduced beneficial impacts.
A diazeniumdiolate-based NO donor induces apoptosis by modulating the CIP2A/PP2A/ERK signaling axis
Authors: Xing Yihao , Hu Yile , Xu Jinglei , Xie Huaxia , Liu Ling 
Published: April 2022

Abstract
Cancerous inhibitor of protein phosphatase 2A (CIP2A) modulates the activity of protein phosphatase 2A (PP2A). PP2A is part of the principal regulators of MAPKs through dephosphorylating tyrosine or threonine residue, resulting in down-regulation of their activities. The present study aimed to assess the inhibition of CIP2A and dephosphorylated effect of PP2A on the ERK signaling pathway in JS-K-induced apoptosis. Cells were noticeably inhibited in a time- and concentration-dependent manner after treatment with JS-K. JS-K could cause an increase in Bax, a decrease in Bcl-2, and subsequent activation of caspase cascade through the release of NO. Meanwhile, JS-K stimulated a significant decrease of CIP2A expression whereas an enhancement of PP2A expression. Furthermore, the effects of JS-K on upregulation of PP2A, downregulation of phosphorylated ERK, and activation of cleaved-caspase-3 and cleaved-PARP were enhanced in CIP2A siRNA-interfered cells. However, overexpression of CIP2A could cancel the effects of JS-K on upregulation of PP2A, downregulation of phosphorylated ERK, and activation of cleaved-caspase-3 and cleaved-PARP. In addition, dephosphorylation of MEK, ERK, Elk-1, c-Myc, and c-Fos stimulated by JS-K was declined in PP2A siRNA cells. Conversely, overexpression of PP2A could strengthen the effects of d dephosphorylation of MEK, ERK, Elk-1, c-Myc, and c-Fos stimulated by JS-K. Simultaneously, the apoptotic cells were reduced in PP2A/C siRNA cells but increased in overexpression of PP2A cells. Taking together, JS-K as a diazeniumdiolate-based NO donor induces apoptosis by modulating the CIP2A/PP2A/ERK signaling axis.
Effect of body mass index on symptomatic venous thromboembolism and prosthesis revision risk after total knee arthroplasty: Data from China
Authors: Shao Changjie , Xu Kuishuai , Zhang Liang , Yu Tengbo , Yu Ning 
Published: April 2022

Abstract
Objective: To investigate the effect of body mass index (BMI) on the risk of symptomatic VTE and prosthesis revision after total knee arthroplasty (TKA). Methods: 7182 patients with primary unilateral TKA treated in our hospital from 2011 to 2020 were divided into four groups according to BMI: BMI<25 kg/m2, BMI 25 kg/m2-29.9 kg/m2, BMI 30 kg/m2-34.9 kg/m2 and BMI≥35 kg/m2. Incidence, Odds ratio and Kaplan-Meier survival analysis were used to evaluate the effects of BMI on symptomatic VTE and prosthesis revision risk after TKA.Results: The incidence of VTE after TKA was 8.9‰（64/7182）. There was no significant difference in the incidence of VTE among different BMI groups（P=0.452）. Deep vein thrombosis mainly occurred in the distal lower extremities, especially in intermuscular veins. Revision rate of prosthesis after TKA was 6.4‰（46/7182）. There was no significant difference in revision rate among different BMI groups（P=0.718）.In the univariate analysis of TKA, there was no increased risk of postoperative VTE and prosthesis revision in patients with different obesity categories compared with patients with normal BMI. Kaplan-Meier curve showed that the prosthesis survival rate of patients with BMI≥35 kg/m2 was relatively low, but the difference was not statistically significant.Conclusions: The higher BMI was not associated with the increased risk of symptomatic VTE and prosthesis revision after TKA. When TKA was used for appropriate indications, high BMI should not be considered as a contraindication.
Risk factors of venous thromboembolic events among diffuse large B-cell lymphoma patients receiving first-line treatment
Authors: Jokimäki Anna , Tokola Susanna , Harmanen Minna , Kuitunen Hanne , Sillanpää Anni , Rönkä Aino , Selander Tuomas , Jukkola Arja , Auvinen Päivi , Kuittinen Outi , Kuusisto Milla E.L. 
Published: April 2022

Abstract
Venous thromboembolic events (VTEs) remain a major cause of mortality and morbidity among lymphoma patients. In this retrospective study, we analyzed the risk factors for VTEs in DLBCL (diffuse large B-cell lymphoma) patients treated with first-line (1L) chemoimmunotherapy in Kuopio and Oulu University Hospitals (2010–2019).VTE occurred in 17.2% (n = 59) in the whole population, and seven VTEs occurred during anticoagulant therapy. Statistically significant risk factors for VTE included age > 65 years, ECOG (Eastern Cooperative Oncology Group) > 1, thrombophilia, bulky disease, LDH > ULN (lactate dehydrogenase; upper limit of normal) and IPI (International Prognostic Index) 4–5. In a multivariate Cox regression model, history of thrombophilia and bulky disease were independent risk factors for VTE (p = 0.000 and p = 0.032, respectively). VTE at 1L had no significant effect on survival at 2 or 5 years of follow-up. Two different VTE risk scores were tested in DLBCL patients treated in 1L. A risk score 2 (age > 65/LDH > ULN/ ECOG > 1/thrombophilia = 1 p., bulky disease 2 p.) received a higher ROC value (0.680) than risk score 1. In the “high risk” group (5 p., according to risk score 2), VTE risk was increased, at 35.3%.
Modeling Inter-DC Bulk Data Transfer in Optical Networks with Edge Storage
Authors: Yue Shengnan , Lin Xiao , Sun Weiqiang , Hu Weisheng 
Published: April 2022

Abstract
In communication networks that span large geographical regions, transfer of bulk data is often costly and hard to manage. One prominent reason for that is bandwidth contention among long-lasting flows, and between long-lasting and short-lived ones on inter-datacenter links. Delaying the transfer of bulk data to off-peak hours by temporarily storing transfer at edge of the network can help reduce bandwidth contention during peak hours, and improve overall bandwidth utilization and network utility. Although extensive research has been conducted on its use, to date no model has been developed to quantify the performance of storage assisted bulk transfers across multiple congested links. This research gap has limited our understanding of the operation of storage assisted transfer under different network conditions. In this paper, we model bulk data transfer in inter-datacenter optical networks with edge storage, and derive closed-form equations for bulk data transfer on inter-DC links that exhibit diurnal traffic patterns and varying peak hours. Our study reveals that when the data transfer requests arrive uniformly in a day, the probability of a successful transfer increases linearly with the time data is allowed to wait in edge storage. Our study also shows that this probability decreases linearly with β + D, where β and D are the durations of background traffic peak and size of data transfers, respectively, when the network is moderately loaded. And, the benefit of storage will barely increase when the allowed waiting time goes beyond β + D.
Multi-cation exchanges involved in cesium and potassium sorption mechanisms on vermiculite and micaceous structures
Authors: Dubus Julien , Leonhardt Nathalie , Latrille Christelle 
Published: April 2022

Abstract
Vermiculite and micaceous minerals are relevant Cs sorbent in soils and sediments. To understand Cs bioavailability in soils resulting from multi-cation exchanges, Cs sorption onto clay minerals have been carried out in batch experiments with solutions containing Ca2+, Mg2+ and K+. A sequence between a vermiculite and various micaceous structures were achieved by conditioning a vermiculite at various amounts of K. Competing cation exchanges were investigated according to the concentration of Cs. The contribution of K on trace Cs desorption is probed by applying different concentrations of K on Cs-doped vermiculite and micaceous structures. Cs sorption isotherms at chemical equilibrium were combined with elemental mass balances in solution and structural analyses. Cs replace easily Mg2+ > Ca2+ and competes scarcely with K. Cs is strongly adsorbed on the various matrix and a K/Cs ratio about a thousand is required to remobilize Cs. Cs is exchangeable as long as the clay interlayer space remains open to Ca2+. However, excess of K, as well as Cs, in solution leads to the collapse of the interlayer spaces that locks the Cs into the structure. Once K and/or Cs collapse the interlayer space, the external sorption sites are then particularly involved in Cs sorption. Subsequently, Cs+ exchanges preferentially with Ca2+ rather than Mg2+. Mg2+ is extruded from the interlayer space by Cs+ and K+ adsorption, excluded from short interlayer space and replaced by Ca2+ as Cs+ desorbs.
PCMT1 regulates the migration, invasion, and apoptosis of prostate cancer through modulating the PI3K/AKT/GSK-3β pathway
Authors: Yuan Chao , Liu Lin , Du Yang , Chen Zhiyuan , Wang Lei , Liu Zelin , Diao Changhui , Liu Xiuheng 
Published: April 2022

Abstract
Background
Protein L-isoaspartate (D-aspartate) O-methyltransferase(PCMT1) is a repair enzyme that catalyzes the conversion of isomerized aspartic acid(iso-Asp) residues into their normal structure, thereby restoring the configuration and function of proteins. Studies have shown that PCMT1 is overexpressed in several tumors and affects patients’ prognosis. However, there are few reports on the role of PCMT1 in prostate cancer (PCa).
Methods
The expression of PCMT1 in prostate cancer cell lines was detected by quantitative reverse transcription-polymerase chain reaction(qRT-PCR) and western blot, and the expression of PCMT1 in prostate cancer tissues was detected by immunohistochemistry staining.A Cell Counting Kit-8(CCK-8) assay, wound-healing assay, transwell migration assay, transwell invasion assay,and flow cytometry assay were conducted to analyze the proliferation, migration, invasion,and apoptosis of prostate cancer cells. The expression of relevant genes was detected by western blot.
Results
The results showed that PCMT1 expression was significantly increased in prostate cancer tissues and cell lines. In PCa clinical samples, PCMT1 expression was closely related to Gleason score, clinical stage, lymph node metastasis and bone metastasis. PCMT1 inhibition significantly suppressed the proliferation, migration and invasion of prostate cancer cells, and promoted apoptosis.We furthermore confirmed that PCMT1 regulated the migration, invasion and apoptosis of PCa cells by modulating the phosphatidylinositol 3-kinase/AKT kinase/glycogen-synthase kinase-3β (PI3K/AKT/GSK-3β) signaling pathway.
Conclusions
PCMT1 plays a cancer-facilitative role in prostate cancer by promoting the proliferation, migration and invasion of prostate cancer cells, and inhibiting apoptosis. Therefore, PCMT1 is considered to represent a novel target for treating prostate cancer.
Isobavachalcone Activates Antitumor Immunity on Orthotopic Pancreatic Cancer Model: A Screening and Validation
Authors: Zhang Hongbo , Liu Xuanming , Cao Jianlin , Zhuo Yuzhen , Jin Jiahui , Gao Qiaoying , Yuan Xiangfei , Yang Lei , Li Dihua , Wang Yan 
Published: April 2022

Abstract
Pancreatic cancer accompanies poor prognosis and accounts for a significant number of deaths every year. Due to possessing a broad spectrum of bioactivities such as antioxidant and antitumor activity, Psoralea corylifolia L. (PCL) is widely used in clinical Chinese medicine. In the present work, we explored potential antitumor agents of PCL and underlying mechanisms in vivo and vitro experiments. Based on the integration of network pharmacology, protein-protein interaction network, the expression profile of gene, survival analysis, multivariate Cox regression model, and molecular docking, we considered Isobavachalcone (IBC) as a candidate compound. Subsequently, we confirmed that IBC could inhibit Panc 02 cells proliferation and induce apoptosis via increasing ROS production in vitro. Meanwhile, IBC could attenuate the weight of orthotopic pancreatic cancer in mice, increase CD8+ cells and reduce M2 macrophages polarization in the tumor tissue and spleen. IBC also alleviated the proportion of MDSCs in the tumor tissue but had no change in the spleen. In addition, IBC restrained the polarization of RAW 264.7 cells into M2 macrophages by inhibiting the expression of ARG1 and MRC1 and suppressed the expression of ARG1 and TGF-β in BM-derived MDSCs. Thus, IBC can attenuate orthotopic pancreatic cancer growth via activating immune activity and inducing cell apoptosis. This research could be a reference for the antitumor ability of IBC and the treatment of the immunosuppressive tumor microenvironment in pancreatic cancer.
Effect of different pretreatment methods on classification of serum samples measured with 1 H-NMR
Authors: Mohammadkhani Leila Ghiasvand , Khoshkam Maryam , Kompany-Zareh Mohsen 
Published: April 2022

Abstract
Extracting accurate biological information from complex datasets is a main challenge in 1H-NMR-based metabolomics research. One of the crucial steps to achieve this goal is to apply an appropriate pretreatment method before multivariate data analysis in 1H-NMR based metabolomics studies. One of the most important pretreatment methods in metabolomics studies is scaling techniques. In this study, the effect of different pretreatment approaches such as auto-, pareto-, level-, range- and vast-scaling in addition to mean-centering are investigated on both experimental and simulated datasets. The goal is linear classification modeling of the toxicity induced by different doses of graphene oxide (GO) in metabolomics context, employing partial least squares- discriminant analysis) PLS-DA). The experimental dataset includes 1H-NMR spectra of mice serum samples exposed to different doses of GO nano-sheets. Here, it is shown that type of applied pre-treatment method has a considerable effect on data analysis results. In this study, auto-, pareto- and vast-scaling lead to a better separation of classes using PLS-DA modeling and PCA. From the results of this study, it was concluded that there is no general rule for the selection of the best scaling method in the analysis of 1H-NMR metabolomics datasets, and different ways of scaling should be tested.
Knowledge, Perception, and Practice of Student’s Challenge faced towards COVID-19 at Selected Higher Education Institutions in Ethiopia. A cross-sectional web survey
Authors: Bulto Tadesse Weyuma , Debele Birhanu Negeri , Chebo Abdella Kosa , Juta Getachew Yigezu , Werku Birhanu Chalchisa 
Published: April 2022

Abstract
Covid-19 affects most of higher education group of peoples; students at higher institutions must be equipped with knowledge, perception, and practice on anticipation measures and ways of transmission. The point of this investigation is to determine the level of knowledge, perception, and practices of students toward COVID-19 at selected Ethiopian higher education institutions. A cross-sectional web survey was conducted from August 10, 2020, to August 30, 2020. The instrument consisted of 8 items on Demographic characteristics, 15 items on knowledge, 4 items on perception, 15 items on practices, and 3 items information usages, changed from a formerly published survey on COVID-19. Descriptive statistics, t-tests, and one-way analysis of variance (ANOVA) were conducted. The result revealed that from the total 342 participants, the majority of students (59.6%) were females, and most of them are young aged below 30 (84.2%). Similarly, most of them were living in Addis Ababa city government 298 (87.1%) and had a degree level of education 244 (71.3%). Most members were likewise playing it safe, for example, staying away from swarms 158 (46.2%) and 83.6% of respondents approached water to wash. In any case, just 37.4% of the respondents have access to infrastructures such as the internet, and 78.4 of the respondent’s approach to laptop or smart-phone. Knowledge, perception, and practice of students play a great role in understanding student’s readiness towards the pandemic of Covid-19. It is also crucial to take actions that help to fight the pandemic. Most of the respondents have access to water for washing, while the majority of them have no sufficient infrastructure for online learning, including the internet, access to laptop, and/or smart-phone during Covid-19. Besides, television and radio, and social media are the most frequently used sources to obtain information about the pandemic.
Rayleigh-Bénard-Marangoni Instability in a Self-rewetting Fluid Layer Overlying a Porous Medium
Authors: Ouyang Zhen , Liu Rong , Ding Zijing 
Published: April 2022

Abstract
Rayleigh-Bénard-Marangoni instability in a bilayer system of self-rewetting fluid overlying a porous medium is investigated. The upper surface of the fluid layer is assumed non-deformable and a constant temperature gradient is imposed. Unlike in previous works, self-rewetting fluid is considered in this paper, whose surface tension is a quadratic function of temperature. As a result, it would reinforce or suppress the instability caused by buoyancy in different scenarios. Thorough study is carried out and the influence of depth ratio h, Rayleigh number Ra and Biot number Bi on instability of the system is discussed. It was found that the marginal curve is bimodal in certain combination of parameters and mode transition of convection is also found.