bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan Media Komik Digital Berbasis STEM Dalam Meningkatkan Kemampuan Literasi Sains Siswa
Authors: Handayani Tri , Winarni Endang Widi , Koto Irwan 
Published: March 2022

Penelitian ini bertujuan untuk mengembangkan media komik digital berbasis STEM dalam meningkatkan kemampuan literasi sains siswa khususnya dimensi proses, konten, dan konteks. Jenis penelitian yang digunakan adalah penelitian dan pengembangan (Research & Development) model 4D (Define, Design, Development, dan Disseminate). Karena penelitian dilakukan pada masa pandemic, tahap penelitian dan pengembangan dilakukan dalam 3 tanpa melaksanakan tahap Disseminate. Data penelitian diperoleh melalui angket, pedoman wawancara, lembar validasi. Aspek yang divalidasi meliputi kelayakan materi, bahasa, dan kegrafikan. Validator (raters) yang dilibatkan untuk memvalidasi aspek media berjumlah 6 pakar yang terdiri dosen dan guru. Data aspek kepraktisan media diperoleh dari respon 20 siswa kelas VI dan 1 orang guru. Setelah uji terbatas dilakukan, tes hasil belajar diberikan untuk mengetahui perubahan literasi sain siswa kelas tentang materi Tatasurya pada dimensi yang ditinjau dari dimensi proses, konten, dan konteks. Berdasarkan hasil penelitian dan pembahasan disimpulkan bahwa media komik digital berbasis STEM sangat layak dan praktis digunakan untuk pembelajaran muatan IPA pada tema 9 Menjelajah Tata Surya sub tema 1 karakteristik planet yang ada di tata surya, sub tema 2 mengenai perbandingan dan beda planet yang ada di tata surya, serta sub tema 3 pengelompokan planet menurut garis edar bumi.Keywords: Komik Digital; STEM; Media; Literasi Sains
circSSU72 Promotes Cell Proliferation, Migration and Invasion of Papillary Thyroid Carcinoma Cells by Targeting miR-451a/S1PR2 Axis
Authors: Zhang Zeyu , Xia Fada , Yao Lei , Jiang Bo , Li Xinying 
Published: March 2022

Introduction: Thyroid cancer is the most common endocrine malignancy with Papillary Thyroid Carcinoma (PTC) as the most common pathological type. Due to low mortality but a high incidence, PTC still causes a relatively heavy burden on financial costs, human health, and quality of life. Emerging researches have indicated that circular RNAs (circRNAs) play a significant regulatory role in various cancers, including PTC. However, the functions and mechanisms of circRNAs derived from SSU72 remain unknown.Method: The expression level of circRNAs derived from the exons of SSU72, miR-361–3p, miR-451a, and S1PR2 was evaluated by qRT-PCR assay or western blot assay. The interactions between circSSU72 (hsa_circ_0009294), miR-451a, and S1PR2 were verified by dual-luciferase reporter assay. Effects of circSSU72, miR-451a, and S1PR2 on cell proliferation, migration, and invasion were confirmed by colony formation assay, cell counting kit-8 (CCK-8), wound healing assay, and Transwell assays in vitro.Results: circSSU72 was upregulated in PTC; circSSU72 knockdown inhibited PTC cell proliferation, migration, and invasion. In addition, circSSU72 could negatively regulate miR-451a by functioning as a sponge. circSSU72 promoted PTC cell proliferation, migration, and invasion by targeting miR-451a in vitro. We further found that miR-451a inhibited PTC cell proliferation, migration, and invasion by regulating S1PR2. Overall, the circSSU72/miR-451a/S1PR2 axis might influence PTC cell proliferation, migration, and invasion.Conclusions: Overall, circSSU72 (hsa_circ_0009294)/miR-451a/S1PR2 axis may promote cell proliferation, migration, and invasion in PTC. Thus, circSSU72 may serve as a potential biomarker and therapeutic target for PTC.
First Documented Camelus knoblochi Nehring (1901) and Fossil Camelus ferus Przewalski (1878) From Late Pleistocene Archaeological Contexts in Mongolia
Authors: Klementiev Alexey M. , Khatsenovich Arina M. , Tserendagva Yadmaa , Rybin Evgeny P. , Bazargur Dashzeveg , Marchenko Daria V. , Gunchinsuren Byambaa , Derevianko Anatoly P. , Olsen John W. 
Published: March 2022

Throughout the arid lands of Africa and Eurasia, camelids facilitated the expansion of human populations into areas that would not likely have been habitable without the transportation abilities of this animal along with the organic resources it provides, including dung, meat, milk, leather, wool, and bones. The two-humped, Bactrian, species ofCamelus,C. ferusin its wild state andC. bactrianuswhen domesticated, is much more poorly known in prehistoric archaeological contexts than its single-humped congeneric,C. dromedarius. Our research uses a convergence of evidence approach to analyze reports and remains of Plio-Pleistocene camelids in Central and Northern Asia and trace the latest-known fossil Bactrian relative,Camelus knoblochi, that seems to have survived in the Gobi Desert until the Last Glacial Maximum (ca. 26.5–19 ka). Rock art depictions, some of which may be of Pleistocene age, record the complexity of nascent human-camel interactions and provide the impetus for further archaeological studies of both the origins ofC. bactrianusand its increasingly complex relationships with the highly mobile prehistoric peoples of Central and Northern Asia.
Relationship Between Non-Alcoholic Fatty Liver Disease and Degree of Hepatic Steatosis and Bone Mineral Density
Authors: Xie Ruijie , Liu Mingjiang 
Published: March 2022

BackgroundThe liver and bones are both active endocrine organs that carry out several metabolic functions. However, the link between non-alcoholic fatty liver disease (NAFLD) and bone mineral density (BMD) is still controversial. The goal of this study was to discover if there was a link between non-alcoholic fatty liver disease and bone mineral density in US persons aged 20 to 59 years of different genders and races.MethodsUsing data from the National Health and Nutrition Examination Survey (NHANES) 2017–2018, multivariate logistic regression models were utilized to investigate the association between NAFLD and lumbar BMD. Fitted smoothing curves and generalized additive models were also used.ResultsThe analysis included a total of 1980 adults. After controlling for various variables, we discovered that NAFLD was negatively linked with lumbar BMD. The favorable connection of NAFLD with lumbar BMD was maintained in subgroup analyses stratified by sex, race and age in men, other race and aged 20-29 years. The relationship between NAFLD and lumbar BMD in blacks and people aged 40-49 years was a U-shaped curve with the inflection point: at 236dB/m and 262dB/m. Furthermore, we discovered that liver advanced fibrosis and liver cirrhosis were independently connected with higher BMD, while no significant differences were detected in severe liver steatosis and BMD.ConclusionsOur study found an independently unfavorable relationship between NAFLD and BMD in persons aged 20 to 59. We also discovered a positive link between BMD and advanced fibrosis and cirrhosis. More research is needed to back up the findings of this study and to look into the underlying issues.
Effects of Biogas Residues on Dissipation of Difenoconazole in Paddy Sediment System Under Field Conditions
Authors: Yu Lina , Wen Yong , Luo Xuhui , Xiang Yun , Yuan Xufeng , Pang Sen , Ma Xiaodong , Li Xuefeng 
Published: March 2022

Little is known about whether the application of biogas residues in rice fields will affect the degradation of pesticides. This study investigated the dissipation behavior of the fungicide difenoconazole in paddy water and sediment after the application of a chemical fertilizer or biogas residues. The results showed that the application of biogas residues changes the dissipation of difenoconazole in both paddy water and sediment. The half-lives of difenoconazole in paddy water and sediment with biogas residues were 0.50 and 10.09 days, respectively, while the half-lives of difenoconazole in paddy water and sediment with chemical fertilizer were only 0.22 and 4.64 days, respectively. After biogas residues were applied in a paddy field, no significant changes in pH value of paddy water and sediment and soil microorganisms were observed, but soil organic matter decreased by 30%. The above studies suggest that biogas residues may affect the dissipation of pesticides in paddy field water and sediments, which provides a new focus on the scientific and rational use of biogas residues as organic fertilizer in rice fields.
Pyroptosis-Related lncRNAs Predict the Prognosis and Immune Response in Patients With Breast Cancer
Authors: Yang Xia , Weng Xin , Yang Yajie , Jiang ZhiNong 
Published: March 2022

Background: Breast cancer (BC) is the most common malignant tumor and the leading cause of cancer-related death in women worldwide. Pyroptosis and long noncoding RNAs (lncRNAs) have been demonstrated to play vital roles in the tumorigenesis and development of BC. However, the clinical significance of pyroptosis-related lncRNAs in BC remains unclear.Methods: Using the mRNA and lncRNA profiles of BC obtained from TCGA dataset, a risk model based on the pyroptosis-related lncRNAs for prognosis was constructed using univariate and multivariate Cox regression model, and least absolute shrinkage and selection operator. Patients were divided into high- and low-risk groups based on the risk model, and the prognosis value and immune response in different risk groups were analyzed. Furthermore, functional enrichment annotation, therapeutic signature, and tumor mutation burden were performed to evaluate the risk model we established. Moreover, the expression level and clinical significance of the selected pyroptosis-related lncRNAs were further validated in BC samples.Results: 3,364 pyroptosis-related lncRNAs were identified using Pearson’s correlation analysis. The risk model we constructed comprised 10 pyroptosis-related lncRNAs, which was identified as an independent predictor of overall survival (OS) in BC. The nomogram we constructed based on the clinicopathologic features and risk model yielded favorable performance for prognosis prediction in BC. In terms of immune response and mutation status, patients in the low-risk group had a higher expression of immune checkpoint markers and exhibited higher fractions of activated immune cells, while the high-risk group had a highly percentage of TMB. Further analyses in our cohort BC samples found that RP11-459E5.1 was significantly upregulated, while RP11-1070N10.3 and RP11-817J15.3 were downregulated and significantly associated with worse OS.Conclusion: The risk model based on the pyroptosis-related lncRNAs we established may be a promising tool for predicting the prognosis and personalized therapeutic response in BC patients.
Newborn Screening for Mitochondrial Carnitine-Acylcarnitine Cycle Disorders in Zhejiang Province, China
Authors: Zhou Duo , Cheng Yi , Yin Xiaoshan , Miao Haixia , Hu Zhenzhen , Yang Jianbin , Zhang Yu , Wu Benqing , Huang Xinwen 
Published: March 2022

Background: Disorders of mitochondrial carnitine–acylcarnitine cycle is a heterogeneous group of hereditary diseases of mitochondrial β-oxidation of fatty acids tested in NBS program in Zhejiang province, China. Large-scale studies reporting disorders of mitochondrial carnitine–acylcarnitine cycle among Chinese population in NBS are limited. The aim of this study was to explain the incidence and biochemical, clinical, and genetic characteristics of disorders of mitochondrial carnitine–acylcarnitine cycle in NBS.Methods: From January 2009 to June 2021, 4,070,375 newborns were screened by tandem mass spectrometry. Newborns with elevated C0 levels and/or C0/(C16 + C18) ratios were identified as having CPT1D, whereas those with decreased C0 levels and/or C0/(C16 + C18) ratios and/or elevated C12-C18:1 level were identified as having CPT2D or CACTD. Suspected positive patients were further subjected to genetic analysis. All confirmed patients received biochemical and nutritional treatment, as well as follow-up sessions.Results: Overall, 20 patients (12 with CPT1D, 4 with CPT2D, and 4 with CACTD) with disorders of mitochondrial carnitine–acylcarnitine cycle were diagnosed by NBS. The overall incidence of these disorders was one in 203,518 newborns. In toal, 11 patients with CPT1D exhibited increased C0 levels and C0/(C16 + C18) ratios. In all patients of CPT2D, all long chain acyl-carnitines levels were elevated except for case 14 having normal C12 levels. In all patients with CACTD, all long chain acyl-carnitines levels were elevated except for case 17 having normal C12, C18, and C18:1 levels. Most patients with CPT1D were asymptomatic. Overall, two of 4 patients with CPT2D did not present any clinical symptom, but other two patients died. In 4 cases with CACTD, the disease was onset after birth, and 75% patients died. In total, 14 distinct mutations were identified in CPT1A gene, of which 11 were novel and c.1910C &gt; A (p.S637T), c.740C &gt; T (p.P247L), and c.1328T &gt; C (p.L443P) were the most common mutations. Overall, 3 novel mutations were identified in CPT2 gene, and the most frequent mutation was c.1711C &gt; A (p.P571T). The most common variant in SLC25A20 gene was c.199-10T &gt; G.Conclusion: Disorders of mitochondrial carnitine–acylcarnitine cycle can be detected by NBS, and the combined incidence of these disorders in newborns was rare in Zhejiang province, China. Most patients presented typical acylcarnitine profiles. Most patients with CPT1D presented normal growth and development, whereas those with CPT2D/CACTD exhibited a high mortality rate. Several novel CPT1A and CPT2 variants were identified, which expanded the variant spectrum.
Application of Intracranial Pressure-Directed Therapy on Delayed Cerebral Ischemia After Aneurysmal Subarachnoid Hemorrhage
Authors: Yang Jun , Lu Junlin , Li Runting , Lin Fa , Chen Yu , Han Heze , Yan Debin , Li Ruinan , Li Zhipeng , Zhang Haibin , Yuan Kexin , Li Hongliang , Zhang Linlin , Shi Guangzhi , Zhou Jianxin , Wang Shuo , Zhao Yuanli , Chen Xiaolin 
Published: March 2022

ObjectiveElevated ICP is a well-recognized phenomenon in aneurysmal subarachnoid hemorrhage (aSAH) that has been demonstrated to lead to poor outcomes. Delayed cerebral ischemia (DCI) is the most important reason for a poor clinical outcome after a subarachnoid hemorrhage. DCI is understood as a multifactorial process that evolves over time, largely caused by the sequelae of increased intracranial pressure (ICP). The study aimed to assess how to better define the association between ICP and DCI, and whether rational ICP management can improve the outcome of aSAH patients.MethodsWe prospectively reviewed patients diagnosed with aSAH and received microsurgery clipping at our department from December 2019 to January 2021. Subdural ICP monitoring devices were kept to monitor the ICP. The ICP values were recorded every 1-h epochs. ICP -guided dehydration treatments were routinely performed to control the ICP level of patients after surgery. To evaluate whether the subdural ICP-directed management improved the prognosis of the aSAH patients, we compared the outcome data of the patients in our cohort with those treated at another ward of our hospital at the same period.ResultsIn total, 144 consecutive aSAH patients received microsurgery clipping at our department, 68 of whom underwent ICP monitoring. A total of 11,424 1-h ICP measurements were recorded for the included patients (1.30 years of recordings). Of 68 patients with ICP monitoring, 27 (27/68, 39.7%) patients developed DCI. Univariate analysis showed that higher Hunt-Hess grade (OR 2.138, 95% CI 1.025–4.459, p = 0.043), higher preoperative modified Rankin Scale score (OR 1.534, 95% CI 1.033–2.276, p = 0.034), and the max ICP value of each day value &gt;28.5 mmHg (OR 4.442, 95% CI 1.509–13.082, p = 0.007) were associated with DCI. Also, patients with ICP-directed treatment showed a significantly lower DCI incidence than patients without ICP monitoring.ConclusionOur study suggests that I less than 15 mmHg possibly constitute normal values and that 28.5 mmHg is the ICP threshold most strongly associated with the occurrence of DCI in aSAH patients. Patients who received the ICP-directed treatment presented a lower incidence of DCI. Our findings provide a basis for the recommendation of ICP-directed treatment after aSAH.Trial Registration NumberNCT04785976.
A Novel Nomogram for Predicting Postoperative Liver Failure After Major Hepatectomy for Hepatocellular Carcinoma
Authors: Lei Zhengqing , Cheng Nuo , Si Anfeng , Yang Pinghua , Guo Guangmeng , Ma Weihu , Yu Qiushi , Wang Xuan , Cheng Zhangjun 
Published: March 2022

BackgroundPost-hepatectomy liver failure (PHLF) is the most common cause of mortality after major hepatectomy in hepatocellular carcinoma (HCC) patients. We aim to develop a nomogram to preoperatively predict grade B/C PHLF defined by the International Study Group on Liver Surgery Grading (ISGLS) in HCC patients undergoing major hepatectomy.Study DesignThe consecutive HCC patients who underwent major hepatectomy at the Eastern Hepatobiliary Surgery Hospital between 2008 and 2013 served as a training cohort to develop a preoperative nomogram, and patients from 2 other hospitals comprised an external validation cohort. Least absolute shrinkage and selection operator (LASSO) logistic regression was applied to identify preoperative predictors of grade B/C PHLF. Multivariable logistic regression was utilized to establish a nomogram model. Internal and external validations were used to verify the performance of the nomogram. The accuracy of the nomogram was also compared with the conventional scoring models, including MELD and ALBI score.ResultsA total of 880 patients who underwent major hepatectomy (668 in the training cohort and 192 in the validation cohort) were enrolled in this study. The independent risk factors of grade B/C PHLF were age, gender, prothrombin time, total bilirubin, and CSPH, which were incorporated into the nomogram. Good prediction discrimination was achieved in the training (AUROC: 0.73) and validation (AUROC: 0.72) cohorts. The calibration curve also showed good agreement in both training and validation cohorts. The nomogram has a better performance than MELD and ALBI score models.ConclusionThe proposed nomogram showed more accurate ability to individually predict grade B/C PHLF after major hepatectomy in HCC patients than MELD and ALBI scores.
Spinal Cord Injury: A Systematic Review and Network Meta-Analysis of Therapeutic Strategies Based on 15 Types of Stem Cells in Animal Models
Authors: Shang Zhizhong , Wang Ruirui , Li Dongliang , Chen Jinlei , Zhang Baolin , Wang Mingchuan , Wang Xin , Wanyan Pingping 
Published: March 2022

Objective:The optimal therapeutic strategies of stem cells for spinal cord injury (SCI) are fully explored in animal studies to promote the translation of preclinical findings to clinical practice, also to provide guidance for future animal experiments and clinical studies.Methods:PubMed, Web of Science, Embase, CNKI, Wangfang, VIP, and CBM were searched from inception to September 2021. Screening of search results, data extraction, and references quality evaluation were undertaken independently by two reviewers.Results and Discussion:A total of 188 studies were included for data analysis. Results of traditional meta-analysis showed that all 15 diverse types of stem cells could significantly improve locomotor function of animals with SCI, and results of further network meta-analysis showed that adipose-derived mesenchymal stem cells had the greatest therapeutic potential for SCI. Moreover, a higher dose (≥1 × 106) of stem cell transplantation had better therapeutic effect, transplantation in the subacute phase (3–14 days, excluding 3 days) was the optimal timing, and intralesional transplantation was the optimal route. However, the evidence of current animal studies is of limited quality, and more high-quality research is needed to further explore the optimal therapeutic strategies of stem cells, while the design and implementation of experiments, as well as measurement and reporting of results for animal studies, need to be further improved and standardized to reduce the risk when the results of animal studies are translated to the clinic.Systematic Review Registration: [website], identifier [registration number].
Liquid Metal-Embedded Layered-PDMS Antenna for Flexible and Conformal Applications
Authors: Wang Meng , Liu Zhifu , Dong Jian 
Published: March 2022

This article proposes a dual-band slot IFA antenna using a combined technique of liquid metal (LM) filling and polydimethylsiloxane substrate. The antenna working at 2.4 and 5.8 GHz consists of three liquid metal layers—radiation patch, short circuit stub, and ground plane and can be bent to different radiuses for flexible, conformal, and wearable applications. The vacuum filling method enables realization of a monolithic-integrated substrate. The applicability of this antenna for a wearable wristband is validated through simulation.
Social Isolation, Cognitive Function, and Depression Among Chinese Older Adults: Examining Internet Use as a Predictor and a Moderator
Authors: Li Yunjun , Bai Xiao , Chen Honglin 
Published: March 2022

ObjectivesDespite the theoretical and practical interest in Internet use among older adults, evidence examining the impacts of Internet use on late-in-life health is limited. This study examines how Internet use affects depression and cognitive function in older adults and investigates if Internet use moderates the relationship between social isolation and depression/cognitive function.MethodWe performed regression analyses using data came from the second wave of the China Longitudinal Aging Social Survey of 2016. Our final sample featured 8,835 older adults.ResultsThe results show 11.4% of Chinese older adults often used the Internet to engage in at least one activity. Internet use was negatively associated with depression, but it was positively related to cognitive function. Socially isolated older adults were more likely to have more depressive symptoms and higher level of cognitive function. There was also an interaction effect between Internet use and social isolation on depression/cognitive function. The negative effect of social isolation was stronger for older adults who used the Internet less. The moderating effect of Internet use was significant for both males and females. However, among those who used the Internet more, the depression levels of socially isolated male participants were much lower than female participants.ConclusionsOur results reveal the importance of considering Internet use in buffering the negative effects of social isolation and the associated health burdens for aging populations. Recommendations for service practice and future research are discussed.