bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uncovering Lasonolide A biosynthesis using genome-resolved metagenomics
Authors: Uppal Siddharth , Metz Jackie L. , Xavier René K.M. , Nepal Keshav , Xu Dongbo , Wang Guojun , Kwan Jason C. 
Published: May 2022

AbstractInvertebrates, in particular sponges, have been a dominant source of new marine natural products. For example, lasonolide A (LSA) is a potential anti-cancer molecule isolated from the marine spongeForcepiasp., with nanomolar growth inhibitory activity and a unique cytotoxicity profile against the National Cancer Institute 60 cell line screen. Here, we identified the putative biosynthetic pathway for LSA. Genomic binning of theForcepiasponge metagenome revealed a gram-negative bacterium belonging to the phylum Verrucomicrobia as the candidate producer of LSA. Phylogenetic analysis showed this bacterium, herein namedCandidatusThermopylae lasonolidus, only has 88.78% 16S rRNA identity with the closest relativePedosphaera parvulaEllin514, indicating it represents a new genus. The lasonolide A (las) biosynthetic gene cluster (BGC) was identified as atrans-AT polyketide synthase (PKS) pathway. When compared with its host genome, thelasBGC exhibits a significantly different GC content and penta-nucleotide frequency, suggesting a potential horizontal acquisition of the gene cluster. Furthermore, three copies of the putativelaspathway were identified in the candidate producer genome. Differences between the threelasrepeats were observed including the presence of three insertions, two single-nucleotide polymorphisms and the absence of a stand-alone acyl carrier protein in one of the repeats. Even though the Verrucomicrobial producer shows signs of genome-reduction, its genome size is still fairly large (about 5Mbp) and when compared to its closest free-living relative contains most of the primary metabolic pathways, suggesting that it is in the early stages of reduction.ImportanceWhile sponges are valuable sources of bioactive natural products, a majority of these compounds are produced in small amounts by uncultured symbionts, hampering the study and clinical development of these unique compounds. Lasonolide A (LSA), isolated from marine spongeForcepiasp., is a cytotoxic molecule active at nanomolar concentrations and causes premature chromosome condensation, blebbing, cell contraction and loss of cell adhesion, indicating a novel mechanism of action and making it a potential anti-cancer drug lead. However, its limited supply hampers progression to clinical trials. We investigated the microbiome ofForcepiasp. using culture-independent DNA sequencing to uncover how an uncultured bacterium produces LSA. This provides future opportunities for heterologous expression and cultivation efforts that may minimize LSA’s supply problem.
Multiple roles of Pax6 in corneal limbal epithelial cells and maturing epithelial cell adhesion
Authors: Sunny Sweetu Susan , Lachova Jitka , Dupacova Naoko , Kozmik Zbynek 
Published: May 2022

AbstractMammalian corneal development is a multistep process, including formation of corneal epithelium (CE), endothelium and stroma during embryogenesis followed by postnatal stratification of the epithelial layers, and continuous renewal of the epithelium to replace the most outer corneal cells. Herein we employed Cre-loxPsystem to conditionally deplete Pax6 proteins in two domains of ocular cells, including the ocular surface epithelium (cornea, limbus and conjunctiva) or postnatal CE, viaK14-creorAldh3-cre, respectively. Earlier and broader inactivation of Pax6 in the OSE resulted in thickened OSE with CE and limbal cells adopting the conjunctival keratin expression pattern. More restricted depletion of Pax6 in postnatal CE resulted in the abnormal cornea marked by reduced epithelial thickness despite of increased epithelial cell proliferation. Immunofluorescence studies showed loss of Keratin 12, an intermediate filament and diffused expression of adherens junction components, together with reduced tight junction protein, Zonula occludens-1 (ZO-1). Furthermore, expression of Keratin 14, basal cell marker in apical layers, indicates impaired differentiation of corneal epithelial cells. Collectively, our data demonstrate that Pax6 is essential for maintaining proper differentiation and strong intercellular adhesion in postnatal corneal epithelial cells, whereas limbal Pax6 is required for preventing the outgrowth of conjunctival cells to the cornea.
Financial and economic risk management in coastal land reclamation projects
Authors: Perera Hashan Pubudu , Perera B.A.K.S. , Palihakkara Asha Dulanjalie 
Published: May 2022

Purpose
Coastal land reclamation (CLR) projects have become an effective solution for population growth while creating new market areas and expanding revenue streams. Although a few studies have been conducted on risk management in CLR projects, they had very little prioritisation on financial and economic risk management. Thus, this study aims to manage the financial and economic risks of CLR projects.

Design/methodology/approach
A quantitative research approach consisting of three Delphi rounds was adopted for this study. The findings of this study were analysed and validated using statistical tools.

Findings
This study identified 13 significant financial and economic risk factors in CLR projects, among which poor quality of the sand and soil, delays in making payments, unpredictability of the safety and security of the country and high dredging volumes were the most significant. Most of these risks have to be borne by the client and the contractor. Conducting environmental impact studies, following quality control procedures and increasing social awareness are significant strategies to handle the financial and economic risks of CLR projects.

Originality/value
This study addresses the literature gap pertaining to financial and economic risk management in CLR projects by identifying its overall process, including the identification of significant financial and economic risks based on the severity levels; risk allocation among the client, contractor and consultant; and suitable risk handling strategies for each significant financial and economic risk factor. Moreover, the findings of this study can be used to effectively deal with financial and economic risks in CLR projects while raising society’s awareness.
Slicing vs chunking product-harm crisis: antecedents and firm performance implications
Authors: Pupovac Ljubomir , Carrillat François Anthony , Michayluk David 
Published: May 2022

Purpose
The high prevalence of product-harm crises (PHC) represents a continuing challenge to which firms sometimes react by announcing several smaller recalls (i.e. slicing) but at other times by announcing the recall of all faulty products at once (i.e. chunking). The slicing vs chunking phenomenon has not been identified by prior literature; this study aims to explore two research questions: Why do firms sometimes slice and other times chunk PHC? Do slicing and chunking affect firm performance differently?

Design/methodology/approach
The authors examined recall guidelines from the US National Highway Traffic Safety Administration (NHTSA) and conducted expert interviews as well as a quantitative analysis of 378 product recalls to determine the antecedents of slicing vs chunking. The authors further performed an event study to examine the impact of slicing vs chunking PHCs on firms’ financial performance.

Findings
The authors find that slicing vs chunking is not a deliberate strategy but rather the consequence of firms’ resource availability and constraints. Furthermore, the authors show that larger firms have a lower likelihood of slicing versus chunking. By contrast, larger R&D expenditures, and greater reputation, as well as larger recall sizes, increase the likelihood of slicing versus chunking. Finally, the results reveal that, compared to chunking, slicing PHC has a strong negative impact on firms’ stock value.

Research limitations/implications
The authors relied on recalls in the US automobile industry. A possible extension would be to study the same phenomenon in other industries or other geographical areas. In addition, the results need to be generalized to other types of negative news that can be either decoupled (slicing) or coupled (chunking), especially negative news for which firms have more discretion regarding the timing of their announcements than for product recalls.

Practical implications
As shown by prior research (Eilert et al., 2017), firms should aim to announce recalls quickly in the wake of a PHC. Importantly though, the results indicate that speed should not come at the expense of comprehensiveness in identifying all defective products, so that only one recall is needed. As suggested by our findings about PHC, investors may react negatively to the slicing of other types of negative news; thus, the results suggest how to best communicate to external stakeholders during crises in general.

Originality/value
To the best of the authors’ knowledge, this is the first study that examines why firms sometimes slice and at other times chunk PHC and identifies the performance implications of these two types of recalls in response to PHC.
Empirical study of housing recovery and property abandonment following valley fire in California: insights from neighborhood characteristics and building attributes
Authors: Rezakhani Pejman 
Published: May 2022

Purpose
This paper aims to examine how neighborhood characteristics (income, population composition) and individual building attributes (ownership) affect the recovery period of single-family housing and determine their correlations with property abandonment and changes in residential land use after natural disaster.

Design/methodology/approach
This empirical study focuses on Valley Fire, one of the California’s most destructive wildfires in 2015, and uses assessor, community, demographic and sales data to measure recovery of a panel of single-family houses located in Lake County in California between 2012 and 2020. Several regression and correlation models will be developed to test different hypotheses.

Findings
This study found that: Recovery period is longer than what expected in most existing literature; ownership status significantly affects recovery period; income level is not a significant factor for shortening the recovery period; and minorities may need more assistance for constant recovery. Findings of this research will help identify at risk communities to avoid uneven housing recovery and lower the rate of property abandonment.

Originality/value
Housing recovery is key to revitalizing communities following major natural disasters. The sociodemographic characteristics of each neighborhood have significant impact on the duration of recovery and possible property abandonment. Understanding how home and neighborhood characteristics affect recovery will help planners prevent long-lasting adverse effects of natural disasters.
Understanding compliance intention of SNS users during the COVID-19 pandemic: a theory of appraisal and coping
Authors: Li Ping , Chang Younghoon , Wang Shan , Wong Siew Fan 
Published: May 2022

PurposeThe purpose of this paper is to explore the factors affecting the intention of social networking sites (SNS) users to comply with government policy during the COVID-19 pandemic.Design/methodology/approachBased on the theory of appraisal and coping, the research model is tested using survey data collected from 326 SNS users. Structural equation modeling is used to test the research model.FindingsThe results show that social support has a positive effect on outbreak self-efficacy but has no significant effect on perceived avoidability. Government information transparency positively affects outbreak self-efficacy and perceived avoidability. Outbreak self-efficacy and perceived avoidability have a strong positive impact on policy compliance intention through problem-focused coping.Practical implicationsThe results suggest that both government and policymakers could deliver reliable pandemic information to the citizens via social media.Originality/valueThis study brings novel insights into citizen coping behavior, showing that policy compliance intention is driven by the ability to cope with problems. Moreover, this study enhances the theoretical understanding of the role of social support, outbreak self-efficacy and problem-focused coping.
A global, continuous calibration curvature strategy for bending sensors of soft fingers
Authors: Gai Ling-Jie , Zong Xiaofeng , Huang Jie 
Published: May 2022

Purpose
The aim of the paper is to propose a global, automated and continuous curvature calibration strategy for bending sensors, which is used for the angle feedback control of soft fingers.

Design/methodology/approach
In this work, the proposed curvature calibration strategy for bending sensors is based on the constant curvature bending properties of soft fingers. The strategy is to install the bending sensor on the soft finger and use the laser distance sensor to assist calibration, then calculate the relationship between the curvature and the voltage of the bending sensor through geometric conversion. In addition, this work also develops a full set of standard calibration systems and collection procedures for the bending sensor curvature calibration and uses machine learning algorithms to fit the collected data.

Findings
First, compared with the traditional calibration methods, the proposed curvature calibration strategy can achieve constant curvature measurement with the advantages of better continuity. Second, using the sensor data obtained by the proposed calibration method as the feedback signal for the soft finger bending angle control, the control effect is better than that of the traditional method.

Originality/value
This work proposes and verifies a global, automated and continuous curvature calibration strategy for bending sensors and is used for the angle feedback control of soft fingers. In addition, this work also develops a full set of standard calibration systems and collection procedures, which can be applied to a variety of flexible bending sensors with a good adaptability.
Blockchain technology acceptance by investment professionals: a decomposed TPB model
Authors: Kumari Anitha , Devi N. Chitra 
Published: May 2022

Purpose
The rapid emergence and acceptance of blockchain applications by investment professionals has made this study significant. The study aims to examine the degree of trust and acceptance of blockchain technology in the Indian financial services industry.

Design/methodology/approach
The decomposed theory of planned behaviour (DTPB) model is investigated using responses from 200 investment professionals to an online survey on blockchain technology adoption, and partial least squares structural equation modelling using SmartPLS 3.0 is used to analyse the results.

Findings
In general, the results support a DTPB and offer the best fit to the data. This study may have significant drawbacks in predicting blockchain technology’s acceptance by investment professionals. Furthermore, the findings suggest that the research instruments used in this study, which engage users in business settings, are equally successful in the context of investment professionals.

Research limitations/implications
In predicting blockchain acceptance by investing experts, this study may have significant drawbacks.

Practical implications
As a result of TAM studies, it has been determined that perceived usefulness is more essential than perceived ease of use. Therefore, investment professionals may have discovered significant subjective normative impacts on behavioural intention. Management must devise techniques for implementing blockchain technology in digital financial services that are compatible with users’ workplaces.

Originality/value
Users may benefit from this research by concentrating on blockchain acceptance to improve digital banking services.
Evolution of modified TAM associated with e-banking services adoption: a systematic PRISMA review from 1975 to 2021
Authors: Naeem Mahpara , Jawaid Syed Tehseen , Mustafa Sadaf 
Published: May 2022

Purpose
This paper aims to review and highlight the gaps in the research streams of the technological acceptance model (TAM) associated with e-banking services. The introduction of TAM as a decision-making process from individual and organizational perspectives is the core purpose of this paper.

Design/methodology/approach
This study is based on concept-centric reviews and synthesizing of previous research. Data are extracted from a systematic literature review published from 1975 to 2021 under the preferred reporting items for systematic review and meta-analyses statement.

Findings
This review explored that theory of reasoned action and theory of planned behavior are the basic theories proceeding to TAM evolution. TAM has been extended to its three versions, which are designed and modified for different contexts and cultures. Previously, the risk-return approach, theory of trust and perceived value were the major constructs or modifications in TAM. Now, TAM has been designed for measuring customers’ perception of any technological advancement.

Research limitations/implications
This review is limited to major additive constructs in modified TAM concerning e-banking services, which can be expanded to different cultures and contexts. This study sketched TAM as a decision-making model associated with the factors influencing any technological advancement. So, the proposed conceptual framework is applicable for the behavioral analysis of technological adoption from individual and organizational perspectives in any field.

Originality/value
This review designed a bi-dimensional conceptual model of TAM as a decision-making process for e-services that has not been identified yet in any study from organizations’ and customers’ perspectives.
Going beyond anti-racist pedagogical practices: co-constructing a pro-Black classroom
Authors: Parks Siettah , Bell Jordan , Ellwood Sydoni , Deckman Sherry L. 
Published: May 2022

Purpose
The purpose of this study is to explore the means, rationale, challenges and opportunities of shifting focus from anti-racist to pro-Black educational practice. The authors argue that while anti-racism is necessary, it is insufficient in addressing the deeply entrenched anti-Blackness in US society. The instructor and three student members of a graduate course on Black girlhoods reflect on their time together to better understand the process of developing a classroom specifically for Black students.

Design/methodology/approach
Through a process of collaborative autoethnography, the authors used their reflections as data to identify the practices that served to establish their space as pro-Black and consider how these practices may apply to other contexts.

Findings
The data presented indicate that co-construction, intentionality and care and love are integral to developing a pro-Black classroom. The implementation of these practices in the authors’ graduate course allowed the students to feel seen and affirmed, which contrasts with their previous experiences in higher education.

Originality/value
This paper introduces the concept of the pro-Black classroom space as a pedagogical transformation aimed at preserving Black lives. The authors’ insights demonstrate how concrete practices that not only constitute anti-racist practice, but further challenge anti-Black bias, help to dismantle structural and systemic inequities in academia.
Conceptualizing mental health among diverse Americans
Authors: Haymovitz Ethan , Barrett Kelly , Torres-Conley Brianda , Schaefer Allison , Zimmerman Rebecca , Zisman-Ilani Yaara , Ortega Debora M. 
Published: May 2022

Purpose
A single conceptualization of mental health based on empirical research has yet to be adopted by researchers and practitioners. This paper aims to explore how diverse Americans define mental health. The aim of the study was to build a conceptualization of the term “mental health”, using qualitative and quantitative methods, on the basis of definitions provided by an ethnically diverse sample of lay-people and professionals.

Design/methodology/approach
Concept mapping methods, including multidimensional scaling and hierarchical cluster analysis, were applied to 146 statements generated by 125 participants of diverse American racial and ethnic groups. The resulting concept map was inspected visually, quantitatively and qualitatively.

Findings
Out of the 146 statements, 8 overarching themes emerged from multidimensional scaling and hierarchical cluster analysis. Themes include Well-being, balance, coping, adaptability, relational, self, lack of mental illness and physical. T-tests revealed statistically significant differences on ratings of importance for statements within the theme “Lack of Mental Illness” from those included in “Well-Being”, “Balance” and “Coping.” Statements included in the theme “Lack of Mental Illness” were rated least essential to the construct of mental health. The “Self” cluster appeared at the center of the data visualization, suggesting that Americans believe that self is essential to the construct in question.

Practical implications
This mixed-method study is consistent with prior evidence that mental health and mental illness might best be considered separate constructs (Westerhof and Keyes, 2010). A logical follow-up might examine why the concept of “Self” emerges centrally as it would help mental health practitioners and policymakers to focus their understanding of mental health to improve mental health interventions.

Originality/value
Understanding that the concept of “Self” is central to Americans’ conceptualizing of mental health may help mental health practitioners and policymakers to focus their efforts in delivering targeted mental health interventions.
Development of portable colorimeter for on-site determination of water quality in aquaculture
Authors: Devarayan Kesavan , Kathavarayan Madhan , Theivasigamani Anand , Sukumaran Monikandon , Kandasamy Sabariswaran 
Published: May 2022

Purpose
This study aims to describe the development of a battery-operated portable colorimeter for on-site determination of water quality in aquaculture.

Design/methodology/approach
A simple and economical colorimeter is built using light sources of different wavelength and a light-dependent resistor combined with an electrical circuit. The whole system was fabricated as to fit into the pocket or palm for easy handling. The developed portable colorimeter was calibrated for estimation of nitrite. Further, the performance of developed portable colorimeters was compared with the commercially available colorimeter.

Findings
Three colorimeters with different light sources were developed and calibrated for determination of nitrite in water. Among them, colorimeter with yellow light source exhibited higher potential for determination of nitrite in the range of 0.5–3.5 ppm. Further, the results of the developed colorimeter are comparable with the commercial colorimeter.

Originality/value
The portable colorimeter developed in this study exhibits potential for determination of nitrite in aquaculture. Determination of nitrite at low concentrations is important for assessing the quality of culture as well as wastewater in aquaculture industry. The accuracy, portable nature, economy and simple operation of these portable colorimeters offer opportunity for on-site determination of water quality parameters in aquaculture.