bluuebunny/crossref_metadata_2025_split huggingface dataset:
Open government data awareness: eliciting citizens’ requirements for application design
Authors: Chokki Abiola Paterne , Simonofski Anthony , Frénay Benoît , Vanderose Benoît 
Published: August 2022

Purpose
Over the past decade, governments around the world have implemented open government data (OGD) policies to make their data publicly available, with collaboration and citizen engagement being one of the main goals. However, even though a lot of data is published, only a few citizens are aware of its existence and usefulness. The purpose of this paper is to identify the requirements for an application that raises awareness of Open Government Data (OGD) to citizens.

Design/methodology/approach
This study followed a design research science approach to collect citizens’ requirements for the design of such an application through interviews with 10 citizens and evaluated through user testing with 25 citizens.

Findings
This study identified and validated 11 requirements that can be implemented to raise citizens’ awareness of OGD. The most useful are listing OGD reuses with information about data used and receiving notification when a new OGD reuse is released. Furthermore, the evaluation results provided evidence of the effectiveness of using an application to improve OGD awareness to citizens.

Originality/value
This research provides requirements that can be used by developers to implement a usable tool to raise citizen awareness or by researchers to evaluate applications whose objective is to raise citizen awareness. Finally, it provides a mobile application that can used by developers to showcase their OGD reuses or by researchers to aware citizens of OGD through real-world examples.
Assessing the Effectiveness of Winter Cover Crops for Controlling Agricultural Nutrient Losses
Authors: Getahun Elias , Keefer Laura L. 
Published: August 2022

ABSTRACTA nutrient loss reduction strategy is necessary to guide the efforts of improving water quality downstream of an agricultural watershed. In this study, the effectiveness of two winter cover crops, namely cereal rye and annual ryegrass, is explored as a loss reduction strategy in a watershed that ultimately drains into a water supply reservoir. Using a coupled optimization‐watershed model, optimal placements of the cover crops were identified that would result in the tradeoffs between nitrate‐N losses reduction and adoption levels. Analysis of the 10%, 25%, 50%, and 75% adoption levels extracted from the optimal tradeoffs showed that the cover crop placements would provide annual nitrate‐N loss reductions of 3.0%–3.7%, 7.8%–8.8%, 15%–17.5%, and 20.9%–24.3%, respectively. In addition, for the same adoption levels (i.e., 10%–75%), sediment (1.8%–17.7%), and total phosphorus losses (0.8%–8.6%) could be achieved. Results also indicate that implementing each cover crop on all croplands of the watershed could cause annual water yield reduction of at least 4.8%, with greater than 28% in the months of October and November. This could potentially be detrimental to the storage volume of the downstream reservoir, especially in drought years, if cover crops are adopted in most of the reservoir's drainage area. Evaluating water yield impacts, particularly in periods of low flows, is thus critical if cover crops are to be considered as best management practices in water supply watersheds.
Adaptive cellular response of the substantia nigra dopaminergic neurons upon age‐dependent iron accumulation
Authors: Kwon Kujin , Cho Hwapyeong , Lee Soyeon , Cho Eun Jeong , Yu Weonjin , Kok Catherine Yen Li , Je Hyunsoo Shawn , Kim Jae‐Ick , Cho Hyung Joon , Kwon Taejoon 
Published: August 2022

AbstractProgressive iron accumulation in the substantia nigra in the aged human brain is a major risk factor for Parkinson's disease and other neurodegenerative diseases. Heavy metals, such as iron, produce reactive oxygen species and consequently oxidative stress in cells. It is unclear, however, how neurons in the substantia nigra are protected against the age‐related, excessive accumulation of iron. In this study, we examined the cellular response of the substantia nigra against age‐related iron accumulation in rats of different ages. Magnetic resonance imaging confirmed the presence of iron in 6‐month‐old rats; in 15‐month‐old rats, iron accumulation significantly increased, particularly in the midbrain. Transcriptome analysis of the region, in which iron deposition was observed, revealed an increase in stress response genes in older animals. To identify the genes related to the cellular response to iron, independent of neurodevelopment, we exposed the neuroblastoma cell line SH‐SY5Y to a similar quantity of iron and then analyzed their transcriptomic responses. Among various stress response pathways altered by iron overloading in the rat brain and SH‐SY5Y cells, the genes associated with topologically incorrect protein responses were significantly upregulated. Knockdown of HERPUD1 and CLU in this pathway increased susceptibility to iron‐induced cellular stress, thus demonstrating their roles in preventing iron overload‐induced toxicity. The current study details the neuronal response to excessive iron accumulation, which is associated with age‐related neurodegenerative diseases.
Bayesian Regression Analysis of Skewed Tensor Responses
Authors: Lee Inkoo , Sinha Debajyoti , Mai Qing , Zhang Xin , Bandyopadhyay Dipankar 
Published: August 2022

Abstract
Tensor regression analysis is finding vast emerging applications in a variety of clinical settings, including neuroimaging, genomics, and dental medicine. The motivation for this paper is a study of periodontal disease (PD) with an order-3 tensor response: multiple biomarkers measured at prespecified tooth–sites within each tooth, for each participant. A careful investigation would reveal considerable skewness in the responses, in addition to response missingness. To mitigate the shortcomings of existing analysis tools, we propose a new Bayesian tensor response regression method that facilitates interpretation of covariate effects on both marginal and joint distributions of highly skewed tensor responses, and accommodates missing-at-random responses under a closure property of our tensor model. Furthermore, we present a prudent evaluation of the overall covariate effects while identifying their possible variations on only a sparse subset of the tensor components. Our method promises Markov chain Monte Carlo (MCMC) tools that are readily implementable. We illustrate substantial advantages of our proposal over existing methods via simulation studies and application to a real data set derived from a clinical study of PD. The R package BSTN available in GitHub implements our model.
Transcriptional signature of TP53 biallelic inactivation identifies a group of multiple myeloma patients without this genetic condition but with dismal outcome
Authors: De Ramón Cristina , Rojas Elizabeta A. , Cardona‐Benavides Ignacio J. , Mateos Maria‐Victoria , Corchete Luis A. , Gutiérrez Norma C. 
Published: August 2022

SummaryBiallelic inactivation of TP53 has been included in the definition of double‐hit (DH) multiple myeloma (MM), which entails an ominous prognosis. However, this condition, or even the presence of high‐risk cytogenetic abnormalities, cannot accurately capture the 15%–20% of the MM population with a median overall survival below 24 months. This prompted us to look for other MM patients who might have transcriptional characteristics similar to those with DH‐TP53. In the present study, we analysed RNA‐seq, whole‐genome and whole‐exome sequencing data from 660 newly diagnosed MM (NDMM) patients from the MMRF (Multiple Myeloma Research Foundation) CoMMpass study to characterize the transcriptional signature of TP53 double‐hit (DH‐TP53) MM. We found 78 genes that were exclusively deregulated in DH‐TP53 patients. A score based on these genes identified a group of 50 patients who shared the same transcriptional profile (DH‐TP53‐like group) whose prognosis was particularly unfavourable [median overall survival (OS) < 2 years], despite not harbouring the biallelic inactivation of TP53. The prognostic value of the DH‐TP53 score was externally validated using gene expression data from 850 NDMM patients analysed by microarrays. Furthermore, our DH‐TP53 score refined the traditional prognostic stratification of MM patients according to the cytogenetic abnormalities and International Staging System (ISS).
Preventing anxiety in the children of anxious parents – feasibility of a brief, online, group intervention for parents of one‐ to three‐year‐olds
Authors: Palmer Emily , Woolgar Matt , Carter Ben , Cartwright‐Hatton Sam , Challacombe Fiona L. 
Published: August 2022

BackgroundThe evidence suggests an increased risk of developing anxiety problems in children of anxious parents. The current study explored the feasibility and acceptability of an intervention with anxious parents of young children, to inform the possibility of further trials.MethodsParticipants were recruited through primary and secondary care psychological services and social media. Participants who had a current or recent anxiety disorder and a child aged 12–47 months were included. Assessments of parental and child outcomes occurred at baseline, after the intervention (week‐2) and follow‐up (week‐8). The intervention was delivered in a small group format, in two sessions, one week apart, using videoconferencing.ResultsOut of 32 participants, 30 (94%) attended the full intervention. All found the intervention acceptable and reported it as useful and relevant. There was a reduction in parental depression (MD = 2.63, 95%CI 1.01–4.26), anxiety (MD = 3.93, 95%CI 2.49–5.37) and stress (MD = 4.60, 95% CI 3.02–6.18) and increases in parenting confidence.ConclusionsThe online group intervention was feasible and acceptable. There were moderate to large effects on parental mental health and no adverse effects on children (decline on outcome measures). This indicates that intervening early in parenting with anxious parents is possible and warrants further investigation to establish prevention efficacy with a larger, controlled trial.
Unpacking the challenges of wildlife governance in community‐based conservation programs to promote human–wildlife coexistence
Authors: Kansky Ruth 
Published: August 2022

AbstractOne of the numerous global challenges today is how to promote biodiversity persistence and at the same time reduce poverty and improve livelihoods, especially in multiple‐use landscapes shared by people and wildlife. In such landscapes, where the cost of sharing the landscape with large wildlife can be high, Community Based Natural Resource Management (CBNRM) program is a model promoted to offset negative wildlife impacts. However, such programs, that promote sustainable utilization of wildlife, are not always successful. To better understand how CBNRM governance systems enable or hinder human–wildlife coexistence, I implemented an 11‐weeks participatory co‐learning program in conservancies in the Zambezi region of Namibia. Framing human–wildlife relationships as occurring within complex social–ecological systems, participatory dialogues were used to unpack the system to better understand why human–wildlife conflict remains a challenge after 30 years of CBNRM in Namibia. From listening to communities I identified five key areas requiring improvement: (1) inefficient communication and transfer of information between management and community; (2) lack of policy and process clarity; (3) lack of support, knowledge, resources, and implementation of mitigation measures; (4) lack of accountability by both management and conservancy members; and (5) lack of skills and capacity by both management and conservancy members. To address these problem areas, I propose three key priority actions: (1) harmonize conservancy policies and management practices; (2) improve information flow; and (3) implement an adaptive management mitigation measure program. While this study focused on a small part of Namibia, I expect this methodology and many of the key findings and proposed interventions will be relevant for other areas applying collaborative approaches to improve human–wildlife coexistence.
Azathioprine hypersensitivity syndrome manifesting as subcutaneous Sweet syndrome and acute pancreatitis
Authors: Thomas Jacob L. , Tee Michael W. , Harp Joanna , Magro Cynthia 
Published: August 2022

AbstractAzathioprine (AZA) is a commonly used immunosuppressive therapy that has been implicated in a number of cutaneous and systemic inflammatory reactions. Initiation of AZA has been associated with a hypersensitivity syndrome manifesting as acute pancreatitis and Sweet syndrome. Subcutaneous Sweet syndrome is a rare variant of Sweet syndrome where the dominant localization of inflammation is within the subcutaneous fat; it is commonly associated with underlying myeloproliferative disease. However, it has not been reported in the literature as a cutaneous manifestation of AZA hypersensitivity syndrome. We present a unique case of acute pancreatitis and biopsy‐proven subcutaneous Sweet syndrome following the initiation of AZA with resolution upon discontinuation.
Compound‐heterozygous GRIN2A null variants associated with severe developmental and epileptic encephalopathy
Authors: Strehlow Vincent , Rieubland Claudine , Gallati Sabina , Kim Sukhan , Myers Scott J. , Peterson Vincent , Ramsey Amy J. , Teuscher Daniel D. , Traynelis Stephen F. , Lemke Johannes R. 
Published: August 2022

AbstractWe report on an 8‐year‐old girl with severe developmental and epileptic encephalopathy due to the compound heterozygous null variants p.(Gln661*) and p.(Leu830Profs*2) in GRIN2A resulting in a knockout of the human GluN2A subunit of the N‐methyl‐D‐aspartate receptor. Both parents had less severe GRIN2A‐related phenotypes and were heterozygous carriers of the respective null variant. Functional investigations of both variants suggested a loss‐of‐function effect. This is the first description of an autosomal recessive, biallelic type of GRIN2A‐related disorder. Nonetheless, there are marked parallels to two previously published families with severe epileptic encephalopathy due to homozygous null variants in GRIN1 as well as various knockout animal models. Compared to heterozygous null variants, biallelic knockout of either GluN1 or GluN2A is associated with markedly more severe phenotypes in both humans and mice. Furthermore, recent findings enable a potential precision medicine approach targeting GRIN‐related disorders due to null variants.
Lacosamide effects on placental carriers of essential compounds in comparison with valproate: Studies in perfused human placentas
Authors: Berman Erez , Kohn Elkana , Berkovitch Matitiahu , Kovo Michal , Eyal Sara 
Published: August 2022

AbstractObjectiveLacosamide is increasingly being prescribed to pregnant women, although its effects on the developing fetus have not been fully clarified yet. Previously, we have shown that several antiseizure medications, particularly valproate, can affect the expression of carriers of essential compounds in placental cells. Here, our aim was to assess the effect of short ex vivo exposure of human placentas to lacosamide on the expression of carriers of essential nutrients required by the human fetus.MethodsPlacentas were obtained from cesarean deliveries of women with no known epilepsy. Cotyledons were cannulated and perfused over 180 min in the presence of lacosamide at 2.5 μg/ml (10 μmol·L−1, n = 7) or 10 μg/ml (40 μmol·L−1, n = 6), representing low and high therapeutic concentrations, respectively, in the maternal perfusate. Valproate (83 μg/ml, 500 μmol·L−1, n = 6) and the perfusion solution (n = 6) were used as the respective positive and negative controls. A customized gene panel array was used to analyze the expression of carrier genes in the perfused cotyledons.ResultsFollowing a 3‐h perfusion, the mRNA expression of SLC19A1 (encoding the reduced folate carrier 1) was downregulated in placentas treated with 10 μg/ml lacosamide (50%) as compared with the vehicle (p < .05). Across all groups, a significant difference was observed in the expression of SLC19A3 (thiamine transporter 2; 52%, 20%, and 9% decrease by 10 μg/ml lacosamide, 83 μg/ml valproate, and 2.5 μg/ml lacosamide, respectively; p < .05).SignificanceLacosamide at high therapeutic concentrations exerted pharmacological effects on the human placenta. Our findings, if manifested in vivo, suggest that lacosamide could potentially affect folate supply to the fetus and support therapeutic monitoring and careful adjustment of lacosamide plasma concentrations during pregnancy.
Chromosomal assembly of the flat oyster (Ostrea edulis L.) genome as a new genetic resource for aquaculture
Authors: Boutet Isabelle , Alves Monteiro Homère J. , Baudry Lyam , Takeuchi Takeshi , Bonnivard Eric , Billoud Bernard , Farhat Sarah , Gonzales‐Araya Ricardo , Salaun Benoit , Andersen Ann C. , Toullec Jean‐Yves , Lallier François H. , Flot Jean‐François , Guiglielmoni Nadège , Guo Ximing , Li Cui , Allam Bassem , Pales‐Espinosa Emmanuelle , Hemmer‐Hansen Jakob , Moreau Pierrick , Marbouty Martial , Koszul Romain , Tanguy Arnaud 
Published: August 2022

AbstractThe European flat oyster (Ostrea edulis L.) is a native bivalve of the European coasts. Harvest of this species has declined during the last decades because of the appearance of two parasites that have led to the collapse of the stocks and the loss of the natural oyster beds. O. edulis has been the subject of numerous studies in population genetics and on the detection of the parasites Bonamia ostreae and Marteilia refringens. These studies investigated immune responses to these parasites at the molecular and cellular levels. Several genetic improvement programs have been initiated especially for parasite resistance. Within the framework of a European project (PERLE 2) that aims to produce genetic lines of O. edulis with hardiness traits (growth, survival, resistance) for the purpose of repopulating natural oyster beds in Brittany and reviving the culture of this species in the foreshore, obtaining a reference genome becomes essential as done recently in many bivalve species of aquaculture interest. Here, we present a chromosome‐level genome assembly and annotation for the European flat oyster, generated by combining PacBio, Illumina, 10X linked, and Hi‐C sequencing. The finished assembly is 887.2 Mb with a scaffold‐N50 of 97.1 Mb scaffolded on the expected 10 pseudochromosomes. Annotation of the genome revealed the presence of 35,962 protein‐coding genes. We analyzed in detail the transposable element (TE) diversity in the flat oyster genome, highlighted some specificities in tRNA and miRNA composition, and provided the first insight into the molecular response of O. edulis to M. refringens. This genome provides a reference for genomic studies on O. edulis to better understand its basic physiology and as a useful resource for genetic breeding in support of aquaculture and natural reef restoration.
Direct and indirect influences of macrophyte cover on abundance and growth of juvenile Atlantic salmon
Authors: Marsh Jessica E. , Jones John Iwan , Lauridsen Rasmus B. , Grace James B. , Kratina Pavel 
Published: August 2022

Abstract

The relationships between macrophytes and the physical and biological characteristics of the environments that aquatic organisms inhabit are complex. Previous studies have shown that the macrophytes, Ranunculus (subgenus Batrachium), which are dominant in lowland chalk streams and widespread across Europe, can enhance juvenile Atlantic salmon abundance and growth to a greater degree than other physical and biological habitat characteristics. However, mechanistic understanding of how this effect might arise requires consideration of the direct and indirect relationships among habitat characteristics that are likely to be influenced by the presence of macrophyte cover.

We applied structural equation modelling to data collected during a 2‐year in‐river manipulative experiment in the River Frome (southern England, U.K.) designed to quantify the magnitude and the relative importance of direct and indirect influences of Ranunculus cover and other physical and biological variables, including water velocity, water depth, prey biomass and body size, and abundance of con‐ and hetero‐specifics, on abundance and somatic growth of 0+ salmon.

Results indicated a strongly positive direct influence of Ranunculus cover on salmon abundance, as well as positive influences of Ranunculus on velocity heterogeneity and water depth that are indirectly related to decreased salmon abundance. Interestingly, there was no indication of a direct influence of Ranunculus cover on salmon growth, although Ranunculus was indirectly related to increased salmon growth through its positive influence on prey biomass, an effect mediated by velocity heterogeneity and proportion of fast velocities.

These findings provide novel mechanistic insights into the key role of Ranunculus in their native lowland rivers to enhance abundance and improve conditions for multiple food web components. Strategies to maintain or enhance naturally occurring Ranunculus in these rivers are therefore likely to return wide ranging ecosystem benefits, including for species of high conservation value, such as salmon. These mechanistic impacts on habitat heterogeneity and ecosystem productivity could generalise to native macrophytes in other river systems, particularly where habitat is dominated by vegetation in the absence of large substrates.
The relevance and impact of business schools: In search of a holistic view
Authors: Redgrave Samuel Douglas James , Grinevich Vadim , Chao Dorrie 
Published: August 2022

AbstractThe degree to which business schools are relevant and impactful for society has been disputed. Critics that engage in the so‐called ‘relevance problem’ have argued that business schools are preoccupied with academic rigour at the expense of practical relevance, resulting in a lack of societal impact. This systematic literature review synthesizes the fragmented body of knowledge pertaining to the relevance and impact of business schools. Appreciating the contributions of both research and education, this review offers a holistic view that acknowledges the multidimensional nature of business schools. Based on an analysis of 266 journal articles, we present the four main literature streams in this domain. We find limited evidence of cross‐fertilisation between discussions of research and education. However, by acknowledging the contribution of applying a multidimensional lens to the study of business schools, we develop a holistic thematic framework that provides theoretical directions for the future. Using this, we demonstrate four avenues for advancing the business school literature. First, we emphasise the potential of an institutional logics perspective to viewing business schools. Second, we offer a novel proposal for understanding the bridge between research and education. Third, we emphasise the application of a value co‐creation theoretical lens when considering how business schools engage with stakeholders in research and education. Finally, we propose an all‐encompassing stakeholder‐centric definition of relevant and impactful knowledge and advocate for this inclusive definition to conceptually bridge the fragmented discussions of research and education.