bluuebunny/crossref_metadata_2025_split huggingface dataset:
Irrigation Water Management in A Water-Scarce Environment in the Context of Climate Change
Authors: Gabr Mohamed ELsayed , Awad Ahmed , Farres Hesham N. 
Published: July 2022

Abstract
In this study, in the context of climate change simulation models were employed to compute the reference evapotranspiration (ETo), net irrigation water requirement (NIWR) and design a new sprinkler irrigation system for two arid sites (Siwa Oasis and West Elminya fields) inside the 1.5-million acres reclamation project in the Egyptian western desert. The CROPWAT 8 model was used to compute NIWR and the irrigation schedule for the main crops wheat, barley, maize, sugar beet, potato, tomato, and date palm, five scenarios were employed the current (1991-2022) scenario, representative concentration path (RCP) 8.5 greenhouse gas emission for the 2040s, 2060s, 2080s, and 2100s scenarios, corresponding to 1.6, 2.1, 3.3, and 4.4 oC expansion with respect to the current baseline 1991-2022. The WaterGEMS model was utilized to run long-term simulations of hydraulic behavior within pressurized pipe networks to irrigate 43 acres using deep groundwater well. ETo values for the current scenario show 4.56 and 5.7 mm for the Siwa Oasis and West Elminya fields respectively. The climate changes cause an increase of the ETo by 4.6, 5.9, 9.4, and 12.7% for RCP: 8.5 greenhouse gas emissions for the 2040s, 2060s, 2080s, and 2100s scenarios respectively for the Siwa Oasis field. On the other hand, an increased ratio for the ETo by 4.2, 5.4, 8.6, and 11.6% for the West Elminya field respectively. The designed sprinkler system indicated a capacity of 111.4 m3 h-1 and 167 m3 h-1 for Siwa and West Elminya fields, respectively. The study suggests the application of modern irrigation systems and crop patterns for wheat, barley, potato, and sugar beet to save irrigation water.
Differentially expressed circRNA and mRNA profiles of neural stem cells with radiation treatment
Authors: Hong Jiacong , Wang Shan , Cai Zhaopeng , Wang Peng , Xie Zhongyu , Chen Keng 
Published: July 2022

Abstract
Background: Spine metastasis is common but highly problematic in clinical oncology practice. Radiotherapy plays an important role in treatment of spine metastasis but damages nervous tissue, especially neural stem cells (NSCs), and leads to radiation-induced myelopathy. Circular RNA (circRNA) is a type of noncoding RNA that responds to external stimuli and regulates cellular functions. However, the mechanism by which radiotherapy affects NSCs and the role of circRNAs in this process are still unclear.Methods: circRNAs and mRNAs in NSCs treated with or without radiation were detected using next-generation sequencing. RT-PCR assays were performed to confirm the sequencing results and differentially expressed circRNAs. Bioinformatic analyses were conducted to identify the critical circRNAs and mRNAs, as well as the enriched functions and pathways. Moreover, a circRNA-miRNA-mRNA network was constructed to investigate the possible regulatory mechanism.Results: A total of 421 differentially expressed circRNAs and 1602 differentially expressed mRNAs were identified in NSCs after radiotherapy. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the differentially expressed mRNAs and of the host genes of the differentially expressed circRNAs were performed, and several key signaling pathways, such as the MAPK signaling pathway, were identified. Moreover, a circRNA-miRNA-mRNA network focusing on the MAPK signaling pathway was constructed and predicted that chr5:127160496|127165240 could be a critical circRNA in the mechanism underlying the response of NSCs to radiation treatment.Conclusion: Our findings reveal the differentially expressed circRNA and mRNA profiles of NSCs after radiotherapy and suggest that circRNAs may contribute to the pathogenesis of radiation-induced myelopathy.Trial registration: N/A
Major venous repair or reconstruction during laparoscopic pancreatic surgery：a single center’s experience
Authors: Zhu Jie , Zhou Xinhua , Wang Haibiao , Ying Dongjian , Chen Baiwen , Mao Haixiang , Li Hong 
Published: July 2022

Abstract
Background In pancreatic cancer surgery, tumor violation of blood vessels is often considered a contraindication of surgery, especially laparoscopic surgery. We had completed 17 cases of major venous repair or reconstruction during laparoscopic pancreatic surgery, and we considered that this surgical method was safe and feasible based on the skilled laparoscopic techniques.Methods Between January 2014 and March 2022. A prospective cohort of 17 patients underwent major venous repair or reconstruction. Among them, 15 cases underwent laparoscopic pancreaticoduodenectomy (LPD), 1 case underwent laparoscopic distal pancreatectomy (LDP) and 1 case underwent laparoscopic central pancreatectomy (LCP). In all cases the pancreatic tumor invaded either portal veins (PV) or superior mesenteric veins (SMV). 13 cases underwent laparoscopic venous resection and reconstruction and 4 cases underwent venous repair.Results Of 17 patients, 10 (58.8%) were male. The mean age was 67.1 (ranged 57-81). All patients successfully completed the operation without transit to open. The average blocking time of venous resection and reconstruction was 30.1 (ranged 15-41) min and the average time of venous wedge resection and stitching was 24.0 (ranged 18-30) min. After surgery, there were no complications such as portal vein stenosis, bleeding, thrombosis and liver failure. 13 patients died within 2 years because of the tumor recurrence, and 4 patients are currently followed by outpatient visits, with no obvious signs of tumor recurrence.Conclusion Studies have shown that the reconstruction or repair of the major veins under laparoscopic surgery was safe and effective. We recommended that surgeons need to have the basics of open surgery in case laparoscopic surgery cannot be continued, and have proficient laparoscopic surgery techniques combined with extensive training to achieve a learning curve for vascular anastomosis.
Transcriptomic analysis reveals biosynthetic genes and transcription factors related to leaf anthocyanin biosynthesis in Aglaonema commutatum
Authors: Li Ji , Wu Kunlin , Li Lin , Ma Guohua , Fang Lin , Zeng Songjun 
Published: July 2022

Abstract
Backgrounds: Aglaonema commutatum ‘Red Valentine’, as an excellent foliage ornamental plant, is widely used in interior and exterior decoration because of its easy cultivation and management. However, reducing proportions of red color foliage during large-scale production of A. commutatum seedlings is of frequent occurence, which has considerable implications on the ornamental and market value of the plant, and the molecular mechanisms of this phenomenon remain unclear. Results: To explore the molecular basis leading to the variation in leaf color in A. commutatum ‘Red Valentine’, transcriptome sequencing were performed based on the Illumina platform in two different varieties at three leaf developmental stages. We annotated 63621 unigenes and 14186 differentially expressed genes by pairwise comparison. Further, twenty-six anthocyanin biosynthetic structural genes were identified, the PKM values were significantly higher in ‘Red Valentine’ than those in the green mutant at the three stages examined, which is consistent with the high anthocyanin content of‘Red Valentine’ leaves. Positive transcription factors that may involved in regulating anthocyanin biosynthesis were detected by BLAST tool and correlation analysis and a downregulation of these TFs may downregulate the expression of anthocyanin genes. Full-length cDNA of anthocyanin biosynthesis and regulation genes was obtained and phylogenetic trees were performed to ensure the accuracy of the analysis. Conclusion: Our study provide an insight into the further understanding of leaf variation and facilitate the breeding of ornamental cultivars with high-anthocyanins levels in A. commutatum ‘Red Valentine’.
Readiness for hospital discharge after a cesarean section and associated factors among Chinese mothers: a cross-sectional study
Authors: XIA Mei-ling , LIN Wen-xuan , GAO Ling-ling , ZHANG Mao-ling , LI Zhi-yun , ZENG Li-ling 
Published: July 2022

Abstract
Background: Cesarean section increases the risk of adverse physical and psychological conditions in mothers and infants. It is important to comprehensively evaluate the readiness for hospital discharge of mothers and identify the limitations of their preparation.Methods: This study enrolled 357 mothers who underwent cesarean section. The data were collected from September 2020 to March 2021 using a face-to-face survey. We evaluated demographic and obstetric factors, readiness for hospital discharge, quality for discharge teaching, social support, family function, and parenting sense of competence. Multiple linear regression analysis was used to identify independent characteristics that influence bedtime procrastination among junior college nursing students. Multiple linear regression analysis was used to identify independent characteristics that influence readiness for hospital discharge among mothers.Results: The total score of the Readiness for Hospital Discharge Scale-New Mother Form was 136.69 ± 25.25. Multiple linear regression analysis revealed that the quality of discharge teaching and parenting sense of competence, number of cesareans, family function, the status of the pregnant women school attendance, and intraoperative pain degree were independent factors influencing the readiness for hospital discharge (P < 0.05).Conclusion: The readiness for hospital discharge of mothers with cesarean section was in the middle level. Improving quality of discharge teaching, parenting sense of competence, and family function will help improve the readiness for hospital discharge of mothers with cesarean section.Relevance to Clinical Practice: Mothers with cesarean section and their families face postpartum difficulties after discharge. Prenatal empowerment and postpartum care can better improve maternal and child health.Patient or Public Contribution: Mothers with cesarean section completed the validated questionnaires in this study. Obstetricians gave exact diagnostic criteria for postpartum complications. Nurses actively cooperated in the process of data collection, and gave professional views on the interpretation of research results.
Early-to-mid idiopathic Parkinson’s disease shows a more cytotoxic but declined CD8-regulatory peripheral immune profile
Authors: Capelle Christophe , Cire Séverine , Hansen Maxime , Pavelka Lukas , Hedin Fanny , Konstantinou Maria , Revets Dominique , Tslaf Vera , Marques Taina , Baron Alexandre , Domingues Olivia , Zeng Ni , May Patrick , Cosma Antonio , Balling Rudi , Krüger Rejko , Ollert Markus , Hefeng Feng 
Published: July 2022

Abstract
Parkinson’s disease (PD) is the second most common neurodegenerative disease. Brain neuroinflammation plays a role in PD pathogenesis. However, the involvement of the peripheral immune system has not been systematically investigated. Here we analyzed >700 combinatorial immunological features in fresh blood of 28 early-to-mid-stage PD patients and 24 matched controls. We found an enhanced cytotoxic immune profile in idiopathic PD patients (iPD), with a higher frequency of terminally-differentiated effector CD8 T (TEMRA), late-differentiated CD8+ natural killer T cells and neutrophils. This immune profile was intensified by elevated serum granzyme A, reduced percentages of CD8+FOXP3+ regulatory T cells and group 2 innate lymphoid cells with immunosuppressive or tolerance-inducing functions. The frequency of CD8 TEMRA was negatively correlated with disease duration, suggesting a contribution to PD pathogenesis. Our work provides a comprehensive map on disturbed peripheral adaptive and innate immune cells in early-to-mid iPD, proposing easily-accessible candidates for early diagnosis and treatments.
Copper selects for siderophore-mediated virulence in Pseudomonas aeruginosa
Authors: Lear Luke , Hesse Elze , Buckling Angus , Vos Michiel 
Published: July 2022

Abstract
BackgroundIron is essential for almost all bacterial pathogens and consequently it is actively withheld by their hosts. However, the production of extracellular siderophores enables iron sequestration by pathogens, increasing their virulence. Another function of siderophores is extracellular detoxification of non-ferrous metals. Here, we experimentally link the detoxification and virulence roles of siderophores by testing whether the opportunistic pathogen Pseudomonas aeruginosa displays greater virulence after exposure to copper. To do this, we incubated P. aeruginosa under different environmentally relevant copper regimes for either two or twelve days. Subsequent growth in a copper-free environment removed phenotypic effects, before we quantified pyoverdine production (the primary siderophore produced by P. aeruginosa), and virulence using the Galleria mellonella infection model. ResultsCopper selected for increased pyoverdine production, which was positively correlated with virulence. This effect increased with time, such that populations incubated with high copper for twelve days were the most virulent. Replication of the experiment with a non-pyoverdine producing strain of P. aeruginosa demonstrated that pyoverdine production was largely responsible for the change in virulence. ConclusionsWe here show a direct link between metal stress and bacterial virulence, highlighting another dimension of the detrimental effects of metal pollution on human health.
Generation and identification of a wheat–Agropyron cristatum (L.) Gaertn. 3P chromosome addition line and substitution line
Authors: Kuang Ziyuan , Ji Xiajie , Xu Shirui , Han Haiming , Zhang Jinpeng , Zhou Shenghui , Yang Xinming , Li Xiuquan , Li Lihui , Liu Weihua 
Published: July 2022

Abstract
Agropyron cristatum (L.) Gaertn. (2n=4x=28, PPPP), which is a wild relative of common wheat and has a P genome, has many excellent characteristics (for example, it is disease resistant, is stress resistant and is high yielding) and has important utilization value for the genetic improvement of wheat. In this study, the wheat– A. cristatum 3P addition line I-27 and 3P(3B) substitution line I-15 were identified for the first time via in situ hybridization (ISH) and specific molecular marker (EST-STS) from wheat– A. cristatum -derived lines, which laid an representing important material for the utilization of excellent genes from A. cristatum chromosome 3P for wheat genetic improvement. In terms of agronomic traits, that the tiller number and spike length of 3P addition line I-27 were significantly higher than those of Fukuhokomugi. The spike length and spikelet number per spike of 3P substitution line I-15 were significantly greater than those of Fukuho. Additionally, wheat– A. cristatum 3P substitution line I-15 was resistant to wheat leaf rust. The generation of the 3P addition line I-27 and 3P substitution line I-15 was essential for the transfer of the excellent genes of the A. cristatum 3P chromosome into common wheat and resulted in the wheat– A. cristatum addition line containing a complete set of genetic research materials, laying a foundation for comparative studies of the P genome and subsequent fine mapping.
Effect of different injury morphology of the endplate on intervertebral disc degeneration ： Retrospective Cohort Study
Authors: Yu Qiuyu , Han Yaozheng , Huang Liangliang , Guo Zhongyi , Su Lintao , Ma Jun , Kang Hui 
Published: July 2022

Abstract
Objectives: To describe a simplified classification scheme for endplate injury morphology based on T2-weighted magnetic resonance imaging and to examine possible associations between endplate injury morphology and vertebral space and other variables such as Type of fracture and disc degeneration in a group of patients with thoracolumbar fractures. Methods: CT and MRI images of 432 patients with thoracolumbar spine fractures were collected and collated. All injury endplates were classified as “mild injury”, “moderate injury” and “severe injury”. The association between the injury morphology of the endplate and the long-term transition outcome of the patients was demonstrated in the studied population. Results: Most moderate injuries to the endplate (fissure-type injury) (77.8%) and severe injuries (irregular depression-type injury, schmorl's node-type injury) (95.3%) resulted in significant disc degeneration in the distant future (p < 0.001). They also showed significant differences in the height of the anterior margin of the injured spine (p < 0.05) and the intervertebral space height index (p < 0.05). The consistency of observers in the present study was expressed as Kappa ≈ 0.84, Preop (ICC,0.855 to 0.988), Postop (ICC,0.822 to 0.981) and F/U (ICC,0.870 to 0.990). Conclusions: The current study shows that although the area of injury of endplate fissure-type injury is small preoperatively, can cause severe degeneration of the disc in long-term transition, resulting in greater impact of the intervertebral space height and angle.
Short Bowel Syndrome Following Resection of Gangrenous Bowel due to Superior Mesenteric Artery Thrombosis in a Patient with Systemic Lupus Erythematosus: Lessons Learnt in a Resource-Poor Setting
Authors: Maduranga Priyantha , Karunathilake Parackrama , Udayakumara EAD , Maduwantha Prabath , Gunarathne Malinga 
Published: July 2022

Abstract
Introduction
Short Bowel Syndrome (SBS) is an intestinal failure resulting from an inadequate length of the intestine following intestinal resection, which can occur due to other causes such as vascular diseases, neoplasms, or inflammatory bowel disease. Short bowel syndrome following intestinal resection in a patient with systemic lupus erythematosus (SLE) is a therapeutic challenge, given that immunosuppressive therapy, wound healing, nutritional optimization, and other potential complications are taken into consideration.
Case Presentation
A 23-year-old male diagnosed with SLE presented with acute onset severe diffuse abdominal pain for a sixteen-hour duration associated with vomiting who was tachycardic on admission, and the pulse volume was low. He was septic with uncompensated metabolic acidosis with a lactate level of 5.5 mmol/L. The abdomen was distended and had diffuse tenderness with guarding and rigidity, and the bowel sounds were not heard. A lateral decubitus ultrasound scan revealed free air in the abdomen, and the abdominal X-ray showed distended small bowel loops. Given the working diagnosis of bowel obstruction, an exploratory midline laparotomy was done, revealing a small gangrenous bowel. The superior mesenteric artery was resected during the procedure, where thrombosis was observed. The necrosed bowel segment was resected, and the patient had short bowel syndrome. After about one week of in-hospital stay, the patient developed left side acute lower limb ischemia. The lower limb CT-angiogram revealed that the left common femoral artery embolus and the left lower limb muscles were not viable in all three compartments of the left lower limb. The decision was made for an above-knee amputation. The patient was nutritionally optimized with total parenteral nutrition (TPN), which was later converted to peripheral parenteral nutrition (PPN), and an oral diet was gradually started. The electrolytes were continuously monitored and corrected. At the time of writing this report, the patient is still managed inward for three months with PPN and supportive care and planned to insert a peripherally inserted central catheter for continuous PPN after discharge.
Conclusion
SLE can be a cause of SBS secondary to bowel gangrene by thromboembolism, and in the case of SLE patients undergoing bowel resection causing SBS is a challenging but feasible situation in terms of medical management, where all the complications should be identified early and managed till the patient achieves a good gut adaptation.
Free posterior tibial artery perforator flapin head and neck reconstruction after tumor expiration
Authors: LI BaoFei , Shi Yi , Deng Di , Xu Feng , Chen Fei 
Published: July 2022

Abstract
Objectives: The purpose of this study was to examine the anatomical features, clinical applications, and complications of the free posterior tibial flap in head and neck reconstruction.Methods： Patients treated with free posterior tibial artery perforator flap in head and neck surgery were retrospectively viewed. Results： A total of 55 patients underwent reconstruction with a free posterior tibial artery perforator flap. Most of the patients had carcinoma of pharynx, trachea, oral and maxillofacial region. The flap dimension ranged from 7.5 cm2 to 108 cm2. The mean thickness of the flap was 1cm. The average length of the vascular pedicle is about 10 cm. Functional recovery of reconstruction area was studied 5~100 months after surgery. The flap survival rate was 98.1%. Specifically speaking, 3 patients developed Partial flap loss and 1 patient developed Total flap loss. Regarding ablative site complications, 6 patients developed wound infection, 2 of them, and another 2 patients developed pharyngeal fistula in the ablative area. Regarding donor site complications, 7 patients developed delayed wound healing and 5 patients developed skin graft necrosis. None of the patients complained of walking difficulty in daily life.Conclusion: Free posterior tibial artery perforator flap as a relatively novel flap, with stable blood supply, thin thickness, flexible cortex, long vascular pedicle, and concealed donor area, is very suitable for head and neck reconstruction. Though PTAFs harvest will produce a minor donor site morbidity which could be solved mostly by conservative treatment, we think PTAFs will become one of the popular techniques for reconstruction of head and neck soft tissue defects.
Patch Repair for Intestinal Perforation Closure in an Ex-Vivo Porcine Model with Burst Pressure Characterization
Authors: Hojati Ashkhan , Policarpio Joseph , Yerrabelli Rahul S. , Wheeler Matthew B. , Rowitz Blair 
Published: July 2022

Abstract
Objective: We assessed the feasibility of closing small intestine perforations with a mesh patch instead of sutures only. Methods: Nine porcine ex vivo small intestine specimens were incised 0.5 cm to simulate bowel perforations. Control group perforations were sutured closed without mesh. The two experimental groups were closed by mesh sewn to the outside (first group) or inside (second group) of the bowel wall. Each specimen was submerged in normal saline and insufflated until air bubbles leaked from the perforation. That insufflation pressure was recorded as the burst pressure. Results: The control group burst at 78.0±17.9 mmHg. The “patch outside” group burst at 54.7±9.1 mmHg. The “patch inside” group burst at 32.0 ±4.6 mmHg. Only the “patch inside” group had statistically significant inferiority, but both groups trended towards inferiority. Discussion: Preliminary research indicates that patch repair of intestinal perforations is likely inferior to sutures alone, although repair with the patch outside the bowel wall cannot be completely excluded because of the high variability of the data. Further research may try alternate patch types or different variations to find a non-inferior closure method. A sample size of n=9 each would give 80% power to detect a minimal detectable difference of 20 mmHg.