bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED: Exosomal miR‐106a‐5p accelerates the progression of nasopharyngeal carcinoma through FBXW7‐mediated TRIM24 degradation
Authors: Li Chang‐Wu , Zheng Jing , Deng Guo‐Qing , Zhang Yu‐Guang , Du Yue , Jiang Hong‐Yan 
Published: March 2022

AbstractNasopharyngeal carcinoma (NPC) is prevalent in East Asia and causes increased health burden. Elucidating the regulatory mechanism of NPC progression is important for understanding the pathogenesis of NPC and developing novel therapeutic strategies. Nasopharyngeal carcinoma and normal tissues were collected. Nasopharyngeal carcinoma cell proliferation, migration, and invasion were evaluated using CCK‐8, colony formation, wound healing, and transwell assays, respectively. A xenograft mouse model of NPC was established to analyze NPC cell growth and metastasis in vivo. The expression of miR‐106a‐5p, FBXW7, TRIM24, and SRGN was determined with RT‐qPCR and Western blot. MiR‐106a‐5p, TRIM24, and SRGN were upregulated, and FBXW7 was downregulated in NPC tissues and cells. Exosomal miR‐106a‐5p could enter NPC cells, and its overexpression promoted the proliferation, migration, invasion, and metastasis of NPC cells, which were suppressed by knockdown of exosomal miR‐106a‐5p. MiR‐106a‐5p targeted FBXW7 to regulate FBXW7‐mediated degradation of TRIM24. Furthermore, TRIM24 regulated SRGN expression by binding to its promoter in NPC cells. Suppression of exosomal miR‐106a‐5p attenuated NPC growth and metastasis through the FBXW7‐TRIM24‐SRGN axis in vivo. Exosomal miR‐106a‐5p accelerated the progression of NPC through the FBXW7‐TRIM24‐SRGN axis. Our study elucidates novel regulatory mechanisms of NPC progression and provides potential exosome‐based therapeutic strategies for NPC.
Down‐regulated surfactant protein B in obese asthmatics
Authors: Cao Thi Bich Tra , Moon Ji‐Young , Yoo Hyun‐Ju , Ban Ga‐Young , Kim Seung‐Hyun , Park Hae‐Sim 
Published: March 2022

AbstractBackgroundObesity is a common comorbid condition in adult asthmatics and known as a feature of asthma severity. However, the molecular mechanism under obesity‐induced inflammation has not yet been fully understood.ObjectiveConsidering the essential role of hydrophobic surfactant protein B (SP‐B) in lung function, SP‐B was targeted to examine its involvement in the development of obesity‐induced airway inflammation in asthmatics.MethodsThe aim was to examine an alteration in circulating SP‐B according to obesity in adult asthmatics, 129 asthmatics were enrolled and classified into 3 groups (obese, overweight and normal‐weight groups) according to body mass index (BMI). Circulating SP‐B levels were determined by enzyme‐linked immunosorbent assay. Four single nucleotide polymorphisms of SFTPB gene were genotyped. Serum ceramide levels were measured by liquid chromatography‐tandem mass spectrometry.ResultsSignificantly lower serum SP‐B levels were noted in the obese group than in the overweight or normal‐weight group (p = .002). The serum SP‐B level was significantly correlated with serum levels of C18:0 ceramide and transforming growth factor beta 1 as well as BMI (r = −0.200; r = −0.215; r = −0.332, p < .050 for all). An inverse correlation was noted between serum SP‐B and fractional exhaled nitric oxide levels in female asthmatics (r = −0.287, p = .009). Genetic predisposition of the SFTPB gene at 9306 A>G to the obese and overweight groups was noted.ConclusionObesity altered ceramide metabolism leading to pulmonary surfactant dysfunction and impaired resolution of airway inflammation, finally contributing to the phenotypes of obese asthmatics.
Effects of fitness and fatness on age‐related arterial stiffening in people with type 2 diabetes
Authors: Cox Emily R. , Brown Wendy J. , Gajanand Trishan , Bailey Tom G. , Gomersall Sjaan R. , Chachay Veronique S. , Burton Nicola W. , Fassett Robert G. , Cox Stephen V. , Coombes Jeff S. , Keating Shelley E. 
Published: March 2022

SummaryPeople with type 2 diabetes (T2D) are at a greater risk of cardiovascular disease than the general population. Both non‐modifiable (age) and modifiable (low aerobic fitness, high body fatness) factors are separately predictive of cardiovascular risk, although they often occur concomitantly. This study aimed to examine the (1) association between age and arterial stiffness, a subclinical marker of cardiovascular risk; and (2) effects of body fatness and aerobic fitness on age‐related increases in arterial stiffness in people with T2D. Data from 64 individuals with T2D (age 59.8 ± 8.7 years, 40% female, HbA1c8.4 ± 1.6%) were included in this cross‐sectional analysis. Carotid‐femoral pulse wave velocity (cfPWV) was used to quantify arterial stiffness. Aerobic fitness (relative V̇O2peak) was determined via indirect calorimetry during maximal exercise testing. Central body fatness was determined using waist circumference. Data were analysed using hierarchical multiple regressions. After adjustment for sex and duration of T2D, each one standard deviation (SD) increase in age (8.68 years) was associated with a 0.63 m·s−1increase in cfPWV (β = 0.416,p = 0.001). Following adjustment for aerobic fitness and body fatness, the standardized β was unchanged (0.417). A one SD increase in waist circumference (13.9 cm) and relative V̇O2peak(5.3 ml·kg−1·min−1) were associated with a similar magnitude of difference in cfPWV (0.47 m·s−1and −0.44 m·s−1, respectively). Therefore, age is a significant correlate of increased arterial stiffness in T2D, with higher aerobic fitness attenuating, and higher body fatness exacerbating, this increase. Interventions aimed at improving cardiovascular outcomes in people with T2D should target both increased aerobic fitness and reduced body fatness.
Heart transplantation in muscular dystrophy: Single‐center analysis
Authors: Visrodia Parth , Patel Nikhil J , Burford Matthew , Hamilton Michele A. , Patel Jignesh K. , Kobashigawa Jon A. , Kittleson Michelle M. 
Published: March 2022

AbstractIntroductionCardiac involvement may occur in many forms of muscular dystrophy (MD). While cardiac disease may progress to warrant heart transplantation (HTx), there may be contraindications related to extra‐cardiac disease including pulmonary and skeletal muscle involvement that limit overall survival and impairs post‐transplant rehabilitation efforts. This study describes the MD HTx experience at a single high‐volume center.MethodsWe examined the clinical characteristics and outcomes of patients with MD with heart failure (HF) (n = 28), patients with MD status post HTx (n = 20) and non‐MD HTx control group (n = 40) matched 2:1 for age at transplant, sex, listing status, and antibody sensitization.ResultsPatients with MD who underwent HTx had increased ventilator days (2 vs. 1 days, p = .013), increased hospital length of stay (20 vs. 12 days, p = .022), and increased discharge to inpatient rehab (60% vs. 8%, p < .001). By 1 year post HTx, patients with MD more often required assistive devices for walking (55% vs. 10%, p = .01). Nonetheless, post‐HTx survival was similar at 1 year (100% vs. 97.5%, p = .48) and 5 years (95.0% vs. 87.5%, p = .36). Of the HTx recipients with MD, 95% were followed by a neurologist, 60% by a neuromuscular specialist as part of the Muscular Dystrophy Association Clinic at our center.ConclusionTransplantation is a feasible option for patients with MD and advanced HF. MD patients who undergo transplantation may benefit from multidisciplinary specialized care to optimize MD‐related morbidity.
The alpha‐7 nicotinic acetylcholine receptor agonist GTS‐21 engages the glucagon‐like peptide‐1 incretin hormone axis to lower levels of blood glucose in db/db mice
Authors: Meng Qinghe , Chepurny Oleg G. , Leech Colin A. , Pruekprasert Napat , Molnar Megan E. , Collier James Jason , Cooney Robert N. , Holz George G. 
Published: March 2022

AbstractAimTo establish if alpha‐7 nicotinic acetylcholine receptor (α7nAChR) agonist GTS‐21 exerts a blood glucose‐lowering action in db/db mice, and to test if this action requires coordinate α7nAChR and GLP‐1 receptor (GLP‐1R) stimulation by GTS‐21 and endogenous GLP‐1, respectively.Materials and MethodsBlood glucose levels were measured during an oral glucose tolerance test (OGTT) using db/db mice administered intraperitoneal GTS‐21. Plasma GLP‐1, peptide tyrosine tyrosine 1‐36 (PYY1‐36), glucose‐dependent insulinotropic peptide (GIP), glucagon, and insulin levels were measured by ELISA. A GLP‐1R–mediated action of GTS‐21 that is secondary to α7nAChR stimulation was evaluated using α7nAChR and GLP‐1R knockout (KO) mice, or by co‐administration of GTS‐21 with the dipeptidyl peptidase‐4 inhibitor, sitagliptin, or the GLP‐1R antagonist, exendin (9‐39). Insulin sensitivity was assessed in an insulin tolerance test.ResultsSingle or multiple dose GTS‐21 (0.5‐8.0 mg/kg) acted in a dose‐dependent manner to lower levels of blood glucose in the OGTT using 10‐14 week‐old male and female db/db mice. This action of GTS‐21 was reproduced by the α7nAChR agonist, PNU‐282987, was enhanced by sitagliptin, was counteracted by exendin (9‐39), and was absent in α7nAChR and GLP‐1R KO mice. Plasma GLP‐1, PYY1‐36, GIP, glucagon, and insulin levels increased in response to GTS‐21, but insulin sensitivity, body weight, and food intake were unchanged.Conclusionsα7nAChR agonists improve oral glucose tolerance in db/db mice. This action is contingent to coordinate α7nAChR and GLP‐1R stimulation. Thus α7nAChR agonists administered in combination with sitagliptin might serve as a new treatment for type 2 diabetes.
Association between cardiorespiratory fitness and risk of all‐cause and cause‐specific mortality
Authors: Zhao Yang , Sun Haohang , Qie Ranran , Han Minghui , Zhang Ming , Shi Xuezhong , Yang Yongli , Lu Jie , Hu Dongsheng , Sun Liang 
Published: March 2022

AbstractObjectiveThe association of cardiorespiratory fitness (CRF) with all‐cause and cause‐specific mortality remains unclear in Chinese population. This study aimed to evaluate the risk of all‐cause, cardiovascular disease (CVD), cancer and other‐cause mortality in Chinese adults using estimated CRF (eCRF).Patients and MethodsWe analysed data for 15,566 participants aged ≥20 years recruited in The Rural Chinese Cohort Study during 2007 to 2008 and followed for mortality during 2013 to 2014. eCRF was calculated with sex‐specific longitudinal non‐exercise algorithms. Cox proportional‐hazards models were used to calculate hazard ratios (HRs) and 95% confidence intervals (CIs) for all‐cause and cause‐specific mortality risk according to baseline eCRF.ResultsDuring a median of 6.01 years of follow‐up, 859 deaths occurred, including 359 from CVD, 221 from cancer, and 279 from other causes. Each 1 metabolic equivalent increment in eCRF was associated with decreased risk of all‐cause mortality (men: HR 0.70, 95% CI [0.66–0.74]; women: 0.59, [0.54–0.64]); CVD mortality (men: 0.70 [0.64–0.77]; women: 0.55, [0.48–0.62]); and other‐cause mortality (men: 0.68 [0.62–0.75]; women: 0.57, [0.49–0.66]). The area under receiver operating characteristic curve was significantly higher for eCRF than its modifiable components (waist circumference, body mass index and resting heart rate) in predicting all‐cause and cause‐specific mortality incidence (all p < .001).ConclusioneCRF was inversely associated with all‐cause, CVD and other‐cause mortality.
Acquired scoliosis of the cervical spine secondary to vertebral neoplasia in an aged pony gelding
Authors: Fairburn Alison J. , Fews Debra , Mair Tim. S. 
Published: March 2022

SummaryA 20‐year‐old New Forest pony gelding presented for investigation of a firm swelling on the right side of the neck and a variably sized patch of sweating on the right side of the cranial neck. Clinical examination revealed moderate scoliosis with right side convexity centred on the mid‐caudal cervical spine. Initial radiographic examination of the cervical spine showed very subtle heterogeneously increased opacity of the vertebral body of the fifth cervical vertebra (C5). Further investigations were declined by the owner. The pony’s clinical condition deteriorated over the following 6 weeks; at this stage, the pony showed proprioceptive deficits of both fore‐ and hindlimbs (grade 1/5 and 3/5 respectively) and difficulty lowering his head to eat from the ground. Repeat radiographs showed mildly increased opacity of the vertebral body of C5 with a small amount of new bone formation ventrally. Neoplasia of the fifth cervical vertebra was suspected. In view of the clinical deterioration and the likely very poor prognosis, the pony was subjected to euthanasia. Post‐mortem computed tomographic examination showed heterogeneously increased attenuation surrounding small, poorly defined regions of reduced attenuation in the trabecular bone of the vertebral body and right transverse process of C5. There was also moderate, irregular periosteal new bone formation on the fifth and sixth cervical vertebrae with a poorly defined mildly hyperattenuating soft tissue structure adjacent to the right pedicle of C5 and extending into the right side of the vertebral canal, compressing the spinal cord to the left side. Post‐mortem examination revealed tan‐coloured tissue running along the right side of the C5 vertebral body, into the right side of the vertebral canal, compressing the spinal cord to the left side and invading adjacent epaxial muscles. Histopathological examination of this tissue was consistent with an undifferentiated sarcoma. To the authors’ knowledge this is the first report of acquired scoliosis secondary to cervical neoplasia in a horse or pony.
Essential components of care in a multidisciplinary headache center: Perspectives from headache neurology specialists
Authors: Goldman Roberta E. , Damush Teresa M. , Kuruvilla Deena E. , Lindsey Hayley , Baird Sean , Riley Samantha , Burrone BS Laura , Grinberg Amy S. , Seng Elizabeth K. , Fenton Brenda T. , Sico Jason J. 
Published: March 2022

AbstractBackground and ObjectiveComprehensive headache care involves numerous specialties and components that have not been well documented or standardized. This study aimed to elicit best practices and characterize important elements of care to be provided in multidisciplinary headache centers.MethodsQualitative, semi‐structured telephone interviews with a purposive sample of headache neurology specialists from across the US, using open‐ended questions. Interviews were recorded, transcribed, and coded. Coded data were further analyzed using immersion/crystallization techniques for final interpretation.ResultsMean years providing headache care was 17.7 (SD = 10.6). Twelve of the 13 participants held United Council for Neurologic Subspecialties headache certification. Six described their practice site as providing multidisciplinary headache care. Participants explained most of their patients had seen multiple doctors over many years, and had tried numerous unsuccessful treatments. They noted patients with chronic headache frequently present with comorbidities and become stigmatized. All participants asserted successful care depends on taking time to talk with and listen to patients, gain understanding, and earn trust. All participants believed multidisciplinary care is essential within a comprehensive headache center, along with staffing enough headache specialists, implementing detailed headache intake and follow‐up protocols, and providing the newest medications, neuromodulation devices, botulinum toxin injections, monoclonal antibodies, nerve blocks and infusions, and treatment from a health psychologist. Other essential services for a headache center are other behavioral health practitioners providing cognitive behavioral therapy, mindfulness, biofeedback and pain management; and autonomic neurology, neuropsychology, vestibular audiology, sleep medicine, physical therapy, occupational therapy, exercise physiology, speech therapy, nutrition, complementary integrative health modalities, and highly trained support staff.ConclusionWhile headache neurology specialists form the backbone of headache care, experts interviewed for this study maintained their specialty is just one of many types of care needed to adequately treat patients with chronic headache, and this is best provided in a comprehensive, multidisciplinary center.
Internationalization of higher education in a post‐pandemic world: Challenges and responses
Authors: Huang Futao , Crăciun Daniela , de Wit Hans 
Published: March 2022

AbstractThis editorial discusses the impact from the COVID‐19 pandemic on the internationalization of higher education, focusing on what general and specific challenges internationalization of higher education in individual countries and systems has faced and will face, and what strategies they developed to deal with these challenges in a post‐pandemic world. Moreover, it outlines the COVID‐19 pandemic and both institutional and national policies for internationalization of higher education in a comparative and global perspective. Finally, it presents key findings from nine articles that are included in the Special Issue.
α4β7high CD4+ T cells are prone to be infected by HIV‐1 and associated with HIV‐1 disease progression
Authors: Mijiti Zilaiguli , Song Jin‐Wen , Jiao Yan‐Mei , Gao Lin , Ma Hai‐Mei , Guo Xiao‐Yan , Zhang Qing , Guo Yun‐Tian , Ding Jian‐Bing , Zhang Shi‐bin , Wang Fu‐Sheng 
Published: March 2022

AbstractIntroductionTo investigate the characteristics of β7high CD4+ T cells during HIV‐1 infection and the relationship between β7high CD4+ T cells and HIV‐1 disease progress.MethodsThis study enrolled 124 HIV‐1‐infected patients, including 80 treatment naïve patients (TNs), 41 patients who underwent antiretroviral therapy (ARTs), and three long‐term no progression patients (LTNPs). Nineteen matched healthy subjects were included as controls (HCs). The characteristics and frequency of β7high CD4+ T cells were analyzed using flow cytometry. An in vitro culture experiment was used to study HIV‐1 infection of β7high CD4+ T cells. Real‐time polymerase chain reaction was performed to quantify HIV‐1 DNA and CA‐RNA levels.ResultsThe frequency of β7high CD4+ T in the peripheral blood was significantly decreased and negatively correlated with disease progression during chronic HIV‐1 infection. A large proportion of β7high CD4+ T cells showed Th17 phenotype. Furthermore, β7high CD4+ T cells were preferentially infected by HIV‐1 in vitro and in vivo. There were no significant differences of HIV‐1 DNA, and CA‐RNA levels between β7high CD4+ T and β7low CD4+ T subsets in HIV‐1 infected individuals after antiviral treatment.ConclusionThe β7high CD4+ T cells were negatively correlated with disease progression during chronic HIV‐1 infection. β7high CD4+ T cells are susceptible to infection with HIV‐1 and HIV‐1 latent cells.
Bladder cancer prospective cohort study on high‐risk non‐muscle invasive bladder cancer after photodynamic diagnosis‐assisted transurethral resection of the bladder tumor (BRIGHT study)
Authors: Kobayashi Keita , Matsuyama Hideyasu , Kawai Taketo , Ikeda Atsushi , Miyake Makito , Nishimoto Koshiro , Matsushita Yuto , Komura Kazumasa , Abe Takashige , Kume Haruki , Nishiyama Hiroyuki , Fujimoto Kiyohide , Oyama Masafumi , Miyake Hideaki , Inoue Keiji , Mitsui Takahiko , Kawakita Mutsushi , Ohyama Chikara , Mizokami Atsushi , Kuroiwa Hajime 
Published: March 2022

ObjectivesTransurethral resection of bladder tumor with photodynamic diagnosis has been reported to result in lower residual tumor and intravesical recurrence rates in non‐muscle invasive bladder cancer. We aimed to evaluate the usefulness of photodynamic diagnosis‐transurethral resection of bladder tumor combined with oral 5‐aminolevulinic acid hydrochloride for high‐risk non‐muscle invasive bladder cancer.MethodsHigh‐risk non‐muscle invasive bladder cancer patients with an initial photodynamic diagnosis‐transurethral resection of bladder tumor (photodynamic diagnosis group) were prospectively registered between 2018 to 2020. High‐risk non‐muscle invasive bladder cancer cases with a history of initial white‐light transurethral resection of bladder tumor (white‐light group) were retrospectively registered. Propensity score‐matching analysis was used to compare residual tumor rates, and factors that could predict residual tumors at the first transurethral resection of bladder tumor were evaluated.ResultsAnalyses were conducted with 177 and 306 cases in the photodynamic diagnosis and white‐light groups, respectively. The residual tumor rates in the photodynamic diagnosis and white‐light groups were 25.7% and 47.3%, respectively. Factor analysis for predicting residual tumors in the photodynamic diagnosis group showed that the residual tumor rate was significantly higher in cases with a current/past smoking history, multiple tumors, and pT1/pTis. When each factor was set as a risk level of 1, cases with a total risk score ≤1 showed a significantly lower residual tumor rate than cases with a total risk score ≥2 (8.3% vs 33.3%, odds ratio 5.46 [1.81–22.28]).ConclusionsIn high‐risk non‐muscle invasive bladder cancer cases, the odds of a residual tumor after initial photodynamic diagnosis‐transurethral resection of bladder tumor were 0.39‐fold that of the odds of those after initial white‐light transurethral resection of bladder tumor. A risk stratification model could be used to omit the second transurethral resection of bladder tumor in 27% of the cases.
RETRACTED: Effect of wound irrigation on the prevention of surgical site infections: A meta‐analysis
Authors: Fu Chunmei , Meng Linghong , Ma Miguo , Li Na , Zhang Jingcen 
Published: March 2022

AbstractWe performed a meta‐analysis to evaluate the effect of wound irrigation on the prevention of surgical site infections. A systematic literature search up to January 2022 was done and 24 studies included 4967 subjects under surgery at the start of the study; antibiotic irrigation was used with 1372 of them, 1261 were aqueous povidone‐iodine irrigation, and 2334 were saline irrigation or no irrigation for surgical site infections prevention in all surgical populations. We calculated the odds ratio (OR) with 95% confidence intervals (CIs) to evaluate the effect of different wound irrigation on the prevention of surgical site infections by the dichotomous method with a random or fixed‐influence model. Antibiotic irrigation had significantly lower surgical site infections in all surgical populations (OR, 0.48; 95% CI, 0.36‐0.62, P < .001) compared with saline irrigation or no irrigation for the subject under surgery. Aqueous povidone‐iodine irrigation had significantly lower surgical site infections in all surgical populations (OR, 0.40; 95% CI, 0.20‐0.81, P = .01) compared with saline irrigation or no irrigation for the subject under surgery. Antibiotic irrigation and aqueous povidone‐iodine irrigation significantly lowered surgical site infections in all surgical populations compared with saline irrigation or no irrigation for the subject under surgery. Further studies are required.