bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validated LC-MS/MS method for quantitation of solasodine in rat urine and feces: Blocking nonspecific adsorption
Authors: Lu Tiantian , Wang Xiaohong , Zhang Qi , Liu Kun , Xu Tongxin , Wang Quande , Zhao Pengfei , Cheng Zhongzhe 
Published: September 2022

AbstractSolasodine, a steroidal alkaloid, is distributed extensively in Solanaceae plants with multiple biological activities such as neuroprotection, antineoplastic and anticonvulsant activities. However, there is little information about the excretion of intact solasodine in vivo. To investigate its excretion, a reliable LC-MS/MS method for quantitation solasodine in rat urine and feces was established and validated. Sample preparation was carried out by liquid-liquid extraction using MTBE as extractant. Moreover, rat urine was preconditioned with BSA, an anti-adsorptive additive, to prevent the nonspecific binding of solasodine to containers and tubes. The method was validated over the range of 4–2000 ng mL−1. The correlation coefficient (r2) were all above 0.999. The intra- and inter-day precision and accuracy were within 16.9% and between −11.0 and 8.9%, respectively. The recovery of solasodine in urine and feces was in the range of 72.5–80.3 and 75.7–80.2%, respectively. IS-normalized matrix factor ranged from 0.94 to 1.12 with RSD% ≤4.02%. This method was successfully applied to the excretion study of solasodine following oral and intravenous administration.
Double troubleStrongyloidiasis hyper infection and intestinal tuberculosis co-infection-A rare case report
Authors: Manasi  , Soumya B.M  , Kulkarni Vardendra 
Published: September 2022

Strongyloidiasis, a human intestinal parasitic infection is most commonly caused by Strongyloides stercoralis species. Strongyloides Hyperinfection is an accelerated autoinfection occurring in immunocompromised state like tuberculosis, where increased number of infectious filariform larvae are produced. Here, we report a case of 35-year-old male, known case of intestinal tuberculosis, presenting with complaints of pain abdomen, associated with vomiting. Clinical and radiological examination revealed hollow viscous perforation with peritonitis. Surgical ileal resection with anastomosis was performed and histopathological analysis of the specimen revealed eggs and larval and adult forms of Strongyloides stercoralis within crypts and superficial mucosa
Penile Girth Enhancement using Amniotic Membrane in a Rabbit Model
Authors: Ariafar Ali , Karbalay-Doust Saied , Ahmed Faisal , Eslahi Ali , Tayebi Sona 
Published: September 2022

Objectives: This study aimed to evaluate the efficacy of Penile Girth Enhancement (PGE) using Amniotic Membrane (AM) as a graft in a rabbit model. Additionally, stereological studies were used to obtain quantitative histological data regarding the structure of the penis. Methods: In this study, 20 adult male rabbits of similar age and weight were allocated to two sham and surgery+AM groups. Both groups underwent surgery by longitudinal Ishape midline incision of the tunica albuginea on the dorsal surface of the penis. The surgery +AM group underwent PGE by AM graft. The penile length and mid circumference were measured using a Vernier caliper before and two months after the surgery. Stereological studies were used to obtain quantitative histological data regarding the structure of the penis. Results: The mean total volume and diameter of the penis increased in the surgery +AM group (p<0.03 and p<0.04, respectively). The stereological evaluation showed a significant increase in the mean volumes of the tunica albuginea and corpora cavernosa in the surgery +AM group compared to the sham group (p<0.01, p< 0.03). Additionally, the mean volume density of the collagen bundles, muscle fibers, and cavernous sinuses and the total number of fibroblasts and smooth muscle cells increased in the surgery +AM group compared to the sham group (p<0.01, p<0.01, p<0.03, p<0.01, and p<0.05, respectively). No infections, bleedings, or other complications were seen. Conclusions: AM is a method that has appeared promising for material use in penile enhancement. Thus, it may be used for PGE in the future.
Keywords: Amniotic Membrane; Histopathology; Animal; Penile Girth Enhancement.
Misdiagnosis of Extraskeletal Osteochondroma in the Foot
Authors: Lynch-Wong Matthew , Wilson Robert Alistair , Wong-Chung John , Sharaf-Eldin Osama 
Published: September 2022

Cases:
Two women presented with newly growing callosities beneath the first and second metatarsal heads, initially believed to reflect gastrocnemius tightness and plantar plate pathology. In another man, swelling at the posterolateral aspect of the heel was mistaken for a Haglund deformity. Subsequent imaging of each patient led to delayed diagnosis of extraskeletal osteochondroma (ESO). Surgical excision resolved symptoms in all 3 with no recurrence over 12 months later.

Conclusions:
Whenever bony prominences newly develop in soft tissues of the foot, ESO should be suspected and appropriate imaging obtained. We describe physical features to help differentiate ESO from other common causes of foot overload.
Flexor Pollicis Longus Tendon Rupture and Carpal Tunnel Syndrome due to Scaphoid Nonunion Advanced Collapse
Authors: Ikumi Akira , Ichimura Harumitsu , Asanuma Sho , Nagashima Katsuya , Takeuchi Yousuke , Tatsumura Masaki , Mammoto Takeo , Ogawa Takeshi , Yoshii Yuichi , Hirano Atsushi , Yamazaki Masashi 
Published: September 2022

Case:
We report a case of flexor pollicis longus (FPL) tendon rupture and carpal tunnel syndrome due to scaphoid nonunion advanced collapse deformity. Intraoperative findings showed disruption of the palmar joint capsule and a sharp proximal bone fragment protruding into the carpal tunnel. Removal of this proximal fragment and tendon grafting were performed. At the postoperative 2-year follow-up, the patient had no wrist pain, finger numbness, or restriction of thumb motion.

Conclusion:
Our results suggest that minimally invasive surgical procedures, such as proximal pole or osteophyte resection, might be optimal choices for early rehabilitation after tendon repair in cases of FPL tendon rupture due to asymptomatic scaphoid nonunion.
Leader Sets up the Direction for the Spiral of Evolution between Employees and Customers
Authors: Chang Yu Hsin , Hsu Wen Ling 
Published: September 2022

Abstract
After environmental selection, co-evolution produces similar and interdependent relationships of species through cooperation and competition. Likewise, a co-evolution exists between employees and customers in enterprises, while leaders dominate the selection factors. The direction that the leaders set up eliminates the unsuitable employees and customers who fail to bring benefits to the enterprise. Since leaders could largely affect the development of enterprises, this study conducted in-depth interviews with leaders of three small enterprises. Next, based on Grounded Theory, the data gathered were summarized into five categories: (1) interaction, (2) selection factors, (3) benefits, (4) adaptability, and (5) limitation. Then, these categories, which drive the tripartite relationship of leaders, employees, and customers, were merged into two concepts, namely, the internal personal factor and the external enterprise factor. The interaction among these three parties forms a leader-oriented co-evolution spiral between employees and customers. This study believes that under the co-evolution and selection factors formulated by the leaders of the organization, employees, and customers who remain connected in the enterprise would treat each other as family members and friends.
Dietary and lifestyle associations with microbiome diversity
Authors: Watson Katherine M. , Siemens MBSc Kyla N. , Anand Sudarshan , Gardner Ivy H. , Sharpton Thomas J. , Dewey MS Elizabeth N. , Martindale MD Robert , Gaulke Christopher A. , Tsikitis  
Published: September 2022

Abstract

Background
Microbial dysbiosis has been closely linked with colorectal cancer development. However, data is limited regarding the relationship of the mucosal microbiome, adenomatous polyps and dietary habits. Understanding these associations may elucidate pathways for risk stratification according to diet.
Results
Patients undergoing screening colonoscopy were included in our prospective, single center study and divided into adenoma or no adenoma cohorts. Oral, fecal, and mucosal samples were obtained. Microbial DNA was extracted, and amplicon libraries generated using primers for the 16S rRNA gene V4 region. Patient and dietary information was collected. Of 104 participants, 44% presented with polyps, which were predominantly tubular adenomas (87%). Adenoma formation and multiple patient dietary and lifestyle characteristics were associated with mucosal microbiome diversity. Lifestyle factors included age, body mass index, adenoma number, and dietary consumption of red meats, processed meats, vegetables, fruit, grain, fermented foods and alcohol.
Conclusion
In this study we showed associations between dietary habits, adenoma formation and the mucosal microbiome. These early findings suggest that ongoing research into diet modification may help reduce adenoma formation and subsequently the development of CRC.
The effects of Social Media Marketing on Consumers Buying Decision Making Processes evidence from College of Business and Economics Students, Bahir Dar University, Ethiopia
Authors: Dessie Tsadiku Setegne , Baylie Abaynesh Degu , Yimer Ahmed Mohammed , Amera Mekdes Birara 
Published: September 2022

Abstract
The main objective of the study was to investigate the effects of social media marketing on Consumers buying decision making processes. Specifically an explanatory research design was employed to examine the effects of social media Ads, social media WOM, users’ presence period on social media, users’ exposure time on social media, users’ number of social media account and users’ level of trust in social media on a consumers buying decision making processes. To achieve the objective of the study, the primary data was collected from a sample 279 students of college of business and economics at Bahir Dar University through questionnaire by applying a convenience sampling techniques. The collected data from sample students were analysed using descriptive statistics such as frequency percentage & mean and inferential statistics such as correlation and multiple regression on SPSS version 23 software package .The study revealed and concluded that Electronic Word of Mouth (E-WOM), Users' Presence Period, Social Media Ads, Users' level of trust and Users' exposure time have a statistically significant positive influence on a consumers buying decision making processes while users’ number of social media account were not statistical significant factor to determine consumer buying behaviour. Finally the study recommended that marketers should have to; use affiliate marketing, align themselves with other trusted brands, publications & influencers/opinion leaders, keep open and honest communication on social media, offer different benefit such as after sales service to the customer to generate positive word of mouth and should have to use the right combination of video, photo, banners, model and creator of social media that will encourage prospective consumers to purchase.
Consumer perception of brand equity based on environmental sustainability - development and validation of a scale
Authors: Diego Luiz , Holanda Francisco Sandro Rodrigues 
Published: September 2022

Abstract

Brands gradually became the core dimension and strategic asset of branding for organizations of all sizes, and today many companies adopt various forms of green marketing activities as part of their strategies. The objective of this study was to investigate and develop the psychometric properties of precision and validity of a scale for brand evaluation equity based on environmental sustainability. The scale was validated by adopting the standards described in the Standards for Educational and Psychological Testing APA. As samples for validation tests, eight companies were selected from those developing their sustainable business. The data were applied to 262 potential consumers. A scale with 22 items was constructed and validated, and after evaluation by the specialists, 22 items were obtained, divided into four dimensions: Brand quality perception — BQP (6 questions); Strategic Brand Positioning – SBP (10 questions); Willingness to purchase — WtB (3 questions) and Innovation in retail — INM (3 questions). Three items were modified because they presented the CVC at 0.8 and were accepted after the adjustments. The instrument items showed good internal consistency (0.877) regarding their domains. For DIF data, the scale invariably works for older and younger people for almost all items, except item INM 04. This article fills the gap in the literature by developing a scale to assess consumer perception of brand equity and environmental sustainability.
Efficacy and prognosis of multiple neoadjuvant therapy and concurrent chemoradiotherapy in locally advanced cervical cancer
Authors: Sun Yi , Ren Fang , Li Gailing , Hai Panpan , Cao Yuan , Han Pin , Liu Yuchen , Wen Jing , Wang Yuanpei , Cheng Xiaoran 
Published: September 2022

Abstract
Objective
To investigate the short-term efficacy and prognosis of neoadjuvant chemotherapy (arterial interventional chemotherapy and intravenous chemotherapy) combined with surgery and concurrent chemoradiotherapy in patients with locally advanced cervical cancer.
Methods
A total of 187 patients with stage IB3-IIB cervical cancer hospitalized in the First Affiliated Hospital of Zhengzhou University from January 2013 to May 2019 were included in this study. According to the inclusion criteria, these patients were pathologically confirmed to be cervical squamous cell carcinoma, adenosquamous carcinoma or adenocarcinoma and completed the treatment according to the established protocol. According to the treatment methods, they were divided into three groups: neoadjuvant arterial interventional chemotherapy + surgery + chemotherapy group (IA-NAC + RS) which included 40 patients, neoadjuvant intravenous chemotherapy + surgery + chemotherapy group (IV-NAC + RS) which included 63 patients and concurrent chemoradiotherapy group (CCRT) which included 84 patients. The prognosis of the three groups and the chemotherapy efficacy, intraoperative blood loss, operation time and postoperative pathological risk factors of different neoadjuvant therapy groups were compared.
Results
①There were no significant differences in the 3-year overall survival (OS) rate, 3-year progression free survival (PFS) rate, 5-year OS rate, and 5-year PFS rate among the three groups (p > 0.05). ②The chemotherapy response rates of IA-NAC + RS group (37.5%) and IV-NAC + RS group (25.4%) were comparable (p > 0.05). ③The intraoperative blood loss in the IA-NAC + RS group (average 92.13 ± 84.09ml) was lower than that in the IV-NAC + RS group (average 127.2 ± 82.36ml), and the difference between the two groups was statistically significant (p < 0.05). ④The operation time of the IA-NAC + RS group (average 231.43 ± 63.10min) and the IV-NAC + RS group (average 219.82 ± 49.11min)were comparable (p > 0.05). ⑤There were no significant differences between the IA-NAC + RS group and IV-NAC + RS group in postoperative pathological lymph node metastasis, parametrial invasion and involvement of lymphovascular space (p > 0.05).
Conclusions
Neoadjuvant chemotherapy combined with surgery had the same long-term survival benefit as concurrent chemoradiotherapy; neoadjuvant arterial chemotherapy could achieve better short-term efficacy than neoadjuvant intravenous chemotherapy without increasing the risk of postoperative pathological risk factors.
Carcinogenic and non – carcinogenic health risks of heavy metals in drinking water of district Mahendergarh, Haryana, India
Authors: Chahal Kavita , Kumar Suneel , Budhwar Savita , Singh Ranjeet , Panghal Amanjeet , Singh Balvinder 
Published: September 2022

Abstract
In the present research work, the study has been carried out on carcinogenic and non-carcinogenic toxic effects for inhabitants due to exposure to heavy metals through dermal and ingestion of drinking water. Present research data assessment has been carried out for the first time; no record has been collected earlier. The maximum concentration of heavy metals was evaluated for Nickel and Arsenic metals, respectively. The average concentration values of heavy metals were found in increasing order as: Ni > As > Cr > Hg > Mn > Cu > Fe > Cd > Zn > Co = Pb 15.36 > 10.3 > 4.73 > 3.32 > 1.43 > 0.27 > 0.246 > 0.068 > 0.06 mg/l respectively. Also, the highest value of incremental lifetime cancer risk was evaluated due to chromium metal. The Hazard Index > 1 was recorded, concluding that non-carcinogenic health risk via ingestion of water, and the Hazard Index < 1 for dermal contact of water, concluded the low risk of non-carcinogenic health risk. These results disclose a new avenue for the removal of these hazardous metals from drinking water. Also, assist future researchers to plan for a healthy life for living things and the present work can be useful for the development of ideas for potential risk control and management.
Thresholds and Mechanisms of Human Magnetophosphene Perception Induced by Alternating Magnetic Fields
Authors: Legros Alexandre , Nissi Janita , Laakso Ilkka , Kavet Robert , Modolo Julien 
Published: September 2022

Abstract
Time-varying Magnetic Fields (MF) are a cornerstone of major modern healthcare technologies and are also a byproduct of our modern environment. Virtually everyone is exposed to power-frequency MF (50/60 Hz), inducing in our body electric fields and currents, potentially modulating brain function. MF-induced electric fields within the central nervous system can generate flickering visual perceptions (magnetophosphenes), which form the basis of international MF exposure guidelines and recommendations protecting workers and the general public. However, magnetophosphene perception thresholds were estimated 40 years ago in a small, unreplicated study with significant uncertainties and leaving open the question of the underlying mechanism. Here, we report magnetophosphene perception in 81 volunteers exposed to MF (eye or occipital exposure) between 0 and 50 mT at frequencies of 20, 50, 60 and 100 Hz. Frequency-dependent threshold were quantified and support an interaction between induced electric fields and retinal rod cells. Beyond fundamental and immediate implications for international guidelines and recommendations, these results have translational potential for the differential diagnosis of retinal disorders.
Antigenic molecular mimicry in viralmediated protection from cancer: the HIV case.
Authors: Manolio Carmen , Ragone Concetta , Cavalluzzo Beatrice , Mauriello Angela , Tornesello Maria Lina , Buonaguro Franco M. , Megna Angelo Salomone , D’Alessio Giovanna , Penta Roberta , Tagliamonte Maria , Buonaguro Luigi 
Published: September 2022

Abstract
Background: People living with HIV /AIDS (PLWHA) show a reduced incidence for three cancer types, namely breast, prostate and colon cancers. In the present study, we assessed whether a molecular mimicry between HIV epitopes and tumor associated antigens and, consequently, a T cell cross-reactivity could provide an explanation for such an epidemiological evidence.
Methods: Homology between published TAAs and non-self HIV-derived epitopes have been assessed by BLAST homology. Structural analyses have been performed by bioinformatics tools. Immunological validation of CD8+ T cell cross-reactivity has been evaluated ex vivo by tetramer staining.
Findings: Sequence homologies between multiple TAAs and HIV epitopes have been found. High structural similarities between the paired TAAs and HIV epitopes as well as comparable patterns of contact with HLA and TCR α and β chains have been observed. Furthermore, cross-reacting CD8+ T cells have been identified.
Interpretation: This is the first study showing a molecular mimicry between HIV antigens an TAAs identified in breast, prostate and colon cancers. Therefore, it is highly reasonable that memory CD8+ T cells elicited during the HIV infection may play a key role in controlling development and progression of such cancers in the PLWHA lifetime. This represents the first demonstration ever that a viral infection may induce a natural “preventive” anti-cancer memory T cells, with highly relevant implications beyond the HIV infection.
Nonlinear Thermal Radiation Effect on 3D Nanofluid Flow with Convective and Slip Condition via Stretching/Shrinking Surface
Authors: Jagadeesh Shiva , Reddy M. Chenna krishna , Tarakaramu Nainaru , Sivakumar Narsu , Sivajothi Ramalingam 
Published: September 2022

Abstract
A numerical technique for the nonlinear thermal radiation effect on 3D (“Three Dimensional”) nanofluid (NFs) motion through shrinking or stretching surface with convective boundary condition is examined. In this investigation we use the convective and velocity slip conditions. The governing equations were converted into a set of couple non-linear ODE’s by suitable similarity transformations. The converted nonlinear equations are obtained by applying R-K-F (“Range-Kutta-Fehlberg”) procedure along with shooting technique. The physical parameters are explained graphically on velocity, temperature and concentration. Moreover, we found the coefficient of skin friction, rate of heat transfer with various nanofluid parameters. It is very good agreement when compared with previous study.
Fabrication of a 3D high-resolution implant for neural stimulation - challenges and solutions
Authors: Shpun Gal , Farah Nairouz , Chemla Yoav , Markus Amos , Gerber Doron , Zalevsky Zeev , Mandel Yossi 
Published: September 2022

Abstract

Background -
Tissue-integrated micro-electronic devices for neural stimulation hold a great potential in restoring the functionality of degenerated organs, specifically, retinal prostheses, which are aimed at vision restoration. The fabrication process of 3D polymer-metal devices with high resolution and a high aspect-ratio (AR) is very complex and faces many challenges that impair its functionality.
Approach -
Here we describe the optimization of the fabrication process of a bio-functionalized 3D high-resolution 1mm circular subretinal implant composed of SU-8 polymer integrated with dense gold microelectrodes (23µm pitch) passivated with 3D micro-well-like structures (20µm diameter, 3µm resolution). To this end, a nickel (Ni) evaporated silicon (Si) wafer was sequentially spin-coated with SU-8 and photolithographed layer-by-layer, with a sharp electrode formation achieved through a two-step bi-layer lift-off process using LOR/AZ, followed by Cr/Au thin-layer sputter deposition to increase the adhesion. Next, the device was released by overnight Ni wet-etching using nitric acid, after which it was bio-functionalized with N2 plasma treatment and the addition of the bio-adhesion molecule arginine-glycine-aspartic acid (RGD).
Main results - In-vitro
and in-vivo investigations, including SEM and FIB cross section examinations, revealed a good structural design, as well as a good integration of the device in the rat sub-retinal space and cell migration into the wells. The reported process and optimization steps described here in detail can aid in the design and fabrication of similar neural implants.
Conclusions -
The reported process and optimization steps described here in detail can aid in the design and fabrication of retinal prosthetic devices or similar neural implants.