bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemical DNA biosensor for the multianalyte detection of HPV-16 and HPV-18 in oral and cervical lesions
Authors: Chaibun Thanyarat , Thanasapburachot Patcharanin , Chatchawal Patutong , Lee Su Yin , Jiaranuchart Sirimanas , Jearanaikoon Patcharee , Promptmas Chamras , Buajeeb Waranun , Lertanantawong Benchaporn 
Published: January 2022

Abstract
Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers. In this study, we developed an electrochemical DNA biosensor for detection of HPV-16 and HPV-18, which are the 2 most prevalent genotypes for development of oral and cervical cancers. The assay involves the sandwich hybridization of the HPV target to silica-redox dye reporter probe and capture probe, followed by electrochemical detection. The sensor was found to be highly specific and sensitive, with detection limit of 22 fM for HPV-16 and 20 fM for HPV-18, between the range of 1 fM to 1 µM. Evaluation with oral and cervical samples showed that the biosensor result was consistent with the nested PCR /gel electrophoresis detection. The biosensor assay could be completed within 90 minutes. Due to its simplicity, rapidity and high sensitivity, this biosensor could be used as an alternative method for HPV detection in clinical laboratories. [151 words]
Increased ENT2 Expression and Its Association With Altered Purine Metabolism in Different Stages of Colorectal Cancer Cell Lines
Authors: Naes Safaa M. , Ab-Rahim Sharaniza , Mazlan Musalmah , Hashim Nurul Azmir Amir , Rahman Amirah Abdul 
Published: January 2022

Abstract
Background Colorectal cancer (CRC) is one of the most prevalent malignant cancers worldwide. Although the purine metabolism pathway is known to be vital for cancer cells survival mechanism, not much is known on ENT2 role in CRC development and its association with purine metabolites. Hence this study is aimed to determine the level of hypoxanthine phosphoribosyl transferase (HPRT), hypoxanthine, uric acid (UA), and the activity of xanthine oxidase (XO) and relate the findings with the ENT2 expression level in different CRC stages. Methods and results Normal colon cell line; CCD-841CoN and a panel of human CRC cell lines; SW480, HCT15 and HCT116, representing different CRC stages; Dukes’ B, C, and D respectively, have been used to measure HPRT, hypoxanthine/xanthine, UA levels and the activity of XO by biochemical assays. The level of ENT2 gene expression was also performed by qRT-PCR. The levels of HPRT, hypoxanthine were significantly higher (P< 0.05), while XO and UA were lower (P< 0.05) in all CRC stages as compared to the normal colon cells. Furthermore, ENT2 expression was found to be increased in all CRC stages. Despite having the highest level of HPRT and hypoxanthine, ENT2 level is lower in Dukes' D when compared to Dukes' B and C. Conclusion The rate of salvage pathway is increased in CRC development as indicated by the elevated levels of HPRT and hypoxanthine in different CRC stages. Increase ENT2 expression implies its importance in assisting hypoxanthine uptake. This step is vital in order to increase DNA synthesis via hypoxanthine recycling. Thus, ENT2 may be a potential marker in therapeutic development.
Developing an Efficient Secure Query Processing Algorithm on Encrypted Databases using Data Compression
Authors: Taloba Ahmed , Fouly Mohamed Ahmed , Soliman Taysir 
Published: January 2022

Abstract
Distributed computing includes putting aside the data utilizing outsider storage and being able to get to this information from a place at any time. Due to the advancement of distributed computing and databases, high critical data are put in databases. However, the information is saved in outsourced services like Database as a Service (DaaS), security issues are raised from both server and client-side. Also, query processing on the database by different clients through the time-consuming methods and shared resources environment may cause inefficient data processing and retrieval. Secure and efficient data regaining can be obtained with the help of an efficient data processing algorithm among different clients. This method proposes a well-organized through an Efficient Secure Query Processing Algorithm (ESQPA) for query processing efficiently by utilizing the concepts of data compression before sending the encrypted results from the server to clients. We have addressed security issues through securing the data at the server-side by an encrypted database using CryptDB. Encryption techniques have recently been proposed to present clients with confidentiality in terms of cloud storage. This method allows the queries to be processed using encrypted data without decryption. To analyze the performance of ESQPA, it is compared with the current query processing algorithm in CryptDB. Results have proven the efficiency of storage space is less and it saves up to 63% of its space.
AgNPs functionalized with dithizone for the detection of Hg2+ based on surface–enhanced Raman scattering spectroscopy
Authors: Guo Na , Xu Guangda , Zhang Qijia , song peng , Xia Lixin 
Published: January 2022

Abstract
Mercuric ion (Hg2+), a poisonous metal ion that remained in water ecosystems, can severely damage the human central and peripheral nervous system and kidneys. Consequently, rapid and highly sensitive methods to determine trace Hg2+ are meaningful to discuss. We have proposed a novel approach of surface-enhanced Raman scattering (SERS) for the quantitative analysis of Hg2+ in water samples using dithizone (DTZ) as a Raman reporter. DTZ-modified silver nanoparticles (AgNPs) produced a strong SERS signal. In the presence of Hg2+, the DTZ can capture Hg2+ composing a stable structure, resulting in DTZ leaving the surface of the AgNPs, with an accompanying decrease in the signal. The proposed SERS assay showed a linear range of 10−4–10−8 M, with a limit of detection of 9.83 × 10−9 M. The sensor has low detection cost, rapid detection speed, and uncomplicated sample pretreatment. Furthermore, this method can be successfully utilized to detect Hg2+rapidly in water samples, which sheds new light on the detection of Hg2+ in the environment.
Molecular dynamics simulations of the
delta and omicron SARS-CoV-2 spike – ACE2 complexes
reveal distinct changes between both variants
Authors: Socher Eileen , Heger Lukas , Paulsen Friedrich , Zunke Friederike , Arnold Philipp 
Published: January 2022

Abstract
SARS-CoV-2, the virus which causes the COVID-19 pandemic, changes frequently through the ap-pearance of mutations constantly leading to new variants. However, only few variants evolve as dominating and will be considered as “Variants of Concern” (VOCs) by the world health organization (WHO). At the end of 2020 the alpha (B.1.1.7) variant appeared in the United Kingdom and domi-nated the pandemic situation until mid of 2021 when it was substituted by the delta variant (B.1.617.2) that first appeared in India as predominant variant. At the end of 2021, SARS-CoV-2 omi-cron (B.1.1.529) evolved as the dominating variant. Here, we use in silico modeling and molecular dynamics (MD) simulations of the receptor-binding domain of the viral spike protein and the host cell surface receptor ACE2 to analyze and compare the interaction pattern between the wild type, delta and omicron variants. We identified residue 493 in delta (glutamine) and omicron (arginine) with altered binding properties towards ACE2.
Short-Term Clinical Efficacy of Femoral Neck System Versus Multiple Cannulated Compression Screws in the Treatment of Garden III and IV Femoral Neck Fractures
Authors: Zhou Xuefeng , Gu Sichao , Li Li , Xu Lei , Wang Xujin , Wang Gang , Xu Xinzhong , Yang Jiazhao , Fang Shiyuan 
Published: January 2022

Abstract
Femoral neck system (FNS) , as a novel minimally invasive internal fixation device, has been gradually applied in the treatment of femoral neck fracture.However, there are few related clinical studies on FNS at present, especially there is no clinical report on FNS in treating GardenIII and IV femoral neck fractures. The aim of the present study was to compare the short-term clinical efficacy of FNS and multiple cannulated compression screws (MCCS) in the treatment of Garden III and IV femoral neck fractures. The data of 78 patients with femoral neck fracture who were admitted to three teaching hospitals affiliated to Anhui Medical University and received internal fixation with FNS and MCCS from June 2019 to December 2020 were collected for a retrospective study. There were 39 patients in both the FNS and MCCS groups. The basic data, perioperative data were recorded and compared between the two groups of patients. The results of the study are encouraging. The operation time was shorter in FNS group than that in MCCS group (p<0.001). The post-operative partial and complete weight-bearing time was earlier in FNS group than that in MCCS group (p<0.001). The Harris hip score in FNS group was higher than that in MCCS group (p<0.001). The incidence rate of lateral thigh irritation in FNS group and MCCS group was 0 (0/39) and 33.3% (13/39), respectively (χ2=15.600, p<0.001). The length of femoral neck shortening was significantly shorter in FNS group than that in MCCS group (t=-5.093, p<0.001). In conclusion, The application of FNS for Garden III and IV femoral neck fractures can shorten the operation time, reduce the frequency of intraoperative fluoroscopy, and facilitate the recovery of hip joint function, so it provides a novel choice for the treatment of Garden III and IV femoral neck fractures in young people.
Knowledge, Attitude, and Perception on Bequeathing of Bodies for Medical Education and Research among Health Science Students of the University of Health and Allied Sciences.
Authors: Quarshie Henrietta Enam , Maalman Raymond Saa-Eru , Ali Mahamudu Ayamba , Donkor Yaw Otchere , Ampong Kingsley , Quaye Jerry , Safianu Rufai , Ennin Isaac Ekow , Korpisah Joseph K. 
Published: January 2022

Abstract
Abstract
Background: Cadaveric dissection is an established effective teaching method in anatomical science education. Cadaver acquisition for dissection is however based on voluntary body bequeathment. As a result of the increasing numbers of medical schools and students intake, the challenges of inadequate bodies for education became visible in most parts of the world as the main cadaver source remains anonymous corpses in the custody of the state. Cultural and religious beliefs or commercial purposes are among several factors that influence the decision about body donations. This study investigates the knowledge, attitude and perception of body bequeathing among health science students who benefitted or are potential beneficiary of cadaveric studies and identified factors influencing the bequest of bodies in Ghana for educational purposes among students in University of Health and Allied Sciences. Method: This was a cross-sectional descriptive study. The study recruited 513 students in the bachelor programmes for medicine, physician assistantship, nursing, midwifery, pharmacy and allied sciences at various levels. Both closed-and open-ended questions contained in a designed Questionnaire were administered. Result: About Seventy-four percent (74.1%) of respondents had heard of body bequeathal. Majority (98.3%) agreed body bequeathal was important. However, only 39.6% knew the requirements and processes of body bequeathal. Most (>90%) had a negative attitude towards body bequeathal. Conclusion: The study concluded that there was a high awareness of the importance of body bequeathal for medical education and research but very low procedural knowledge on bequeathing a body among health science students. Also most were unwillingness to donate their body or even encouraging others to donate their body. It is therefore recommended that the medical schools should set up accessible body bequeathal programmes that provides opportunities for interested individuals to be readily assisted through the process of body bequeathal.

Keywords: Body Bequeathal, Medical Science Education, Cadaveric Dissection, Anatomical education
Risk Factors of Pregnancy Outcome in Patients with Pulmonary Hypertension
Authors: Zhao Kaixun , Yang Ziyang , Zhou Yin , Xie Nanshan , Lai Disheng , Zhang Caojin 
Published: January 2022

Abstract
OBJECTIVE: The purpose of this study is to explore the factors affecting the pregnancy outcome of patients with pulmonary hypertension, and to design a digital model predicting the pregnancy outcome of patients and judge whether patients have the conditions to continue pregnancy. METHODS: The clinical data of patients with pregnancy complicated with pulmonary hypertension hospitalized in Guangdong Provincial People's Hospital from January 1, 2014 to December 31, 2020 were retrospectively analyzed. The severity of pulmonary hypertension, the structural and functional indexes measured by echocardiography were compared, and the effects on maternal and neonatal outcomes were analyzed. RESULTS: A total of 158 patients met the inclusion and exclusion criteria. The results showed that tricuspid regurgitation velocity (P<0.001), right ventricular diameter (P<0.05), right atrial diameter (P<0.05), pulmonary artery diameter (P<0.001) and cardiac function classification (P<0.001) were risk factors for maternal outcomes; Tricuspid regurgitation velocity (P<0.001) and structural changes of right heart were risk factors for neonatal outcomes. By multivariate logistic regression analysis, tricuspid regurgitation velocity (P<0.001), cardiac function (P<0.05) and superior inferior diameter of right atrium (P<0.05) were significant risk factors for maternal outcomes, while tricuspid regurgitation velocity (P<0.001) and superior inferior diameter of right atrium (P<0.05) were significant risk factors for neonatal outcomes. Using significant risk factors, a risk score system was established to predict the pregnancy outcome. CONCLUSION: in this study, we analyzed the clinical data of patients with pregnancy complicated with pulmonary hypertension in our hospital in recent years, and found that the changes of right heart structure (the acceleration of tricuspid regurgitation velocity, and the increase of right atrium, right ventricular diameter, right ventricular outflow tract anteroposterior diameter and main pulmonary artery diameter), are influencing factors of pregnancy outcomes in patients with pulmonary hypertension. In addition, we preliminarily designed a risk score through multi factor analysis, which is helpful to predict the possible outcome of puerperium and neonatal outcomes of patients, and to provide some reference for clinicians and patients to make decisions on whether to continue pregnancy in clinical practice.
Simulation of COVID-19 Symptoms in a Genetically Engineered Mouse Model
Authors: Singh Mahavir , Pushpakumar Sathnur , Bard Nia , Zheng Yuting , Homme Rubens P. , Tyagi Suresh C. 
Published: January 2022

Abstract
The ongoing infectious viral disease pandemic (also known as the coronavirus disease-19; COVID-19) by a constantly emerging viral agent commonly referred as the severe acute respiratory syndrome corona virus 2 or SARS-CoV-2 has revealed unique pathological findings from infected human beings, and the postmortem observations. The list of disease symptoms, and post-mortem observations is too long to mention; however, a few notable ones are worth mentioning to put into a perspective in understanding the malignity of this pandemic starting with respiratory distress or dyspnea, chest congestion, muscle or body aches, malaise, fever, chills, etc. We opine that further improvement for delivering highly effective treatment, and preventive strategies would be benefited from validated animal disease models. In this context, we designed a study and show that a genetically engineered mouse expressing the human angiotensin converting enzyme 2; hACE2 (the receptor used by SARS-CoV-2 agent to enter host cells) represents an excellent investigative resource in simulating important clinical features of the COVID-19 infection. The hACE2 mouse model (which is susceptible to SARS-CoV-2) when administered with a recombinant SARS-CoV-2 spike (S) protein intranasally exhibited a profound cytokine storm capable of altering the physiological parameters including significant changes in in vivo cardiac function along with multi-organ damage that was further confirmed via histological findings. More importantly, visceral organs from SARS-CoV-2 spike (S) treated mice revealed thrombotic blood clots as seen during postmortem examination of the mice. Thus, the hACE2 engineered mouse appears to be a suitable model for studying intimate viral pathogenesis paving the way for further identification, and characterization of appropriate prophylactics as well as therapeutics for COVID-19 management.
Molecular characterization of acquired resistance to KRAS G12C inhibition in gastrointestinal cancers
Authors: Misale Sandra , Yaeger Rona , Mezzadra Riccardo , Sinopoli Jenna , Bian Yu , Marasco Michelangelo , Kaplun Esther , Gao Yijun , Zhao Huiyong , Paula Arnaud Da Cruz , Zhu Yingjie , Bowker Sydney , Chang Qing , Qeriqi Besnik , Weigelt Britta , Der-Torossian Hirak , Anderes Kenna , Socci Nicholas , Shia Jinru , Riely Greg , Murciano-Goroff Yonina , Li Bob , Christensen James , Reis-Filho Jorge , Solit David , de Stanchina Elisa , Lowe Scott , Rosen Neal 
Published: January 2022

Abstract
KRAS G12C inhibitors, such as sotorasib, have rapidly moved through clinical development and are poised to transform care of patients with KRAS G12C mutant cancers, in particular non-small cell lung cancer (NSCLC) and colorectal cancer (CRC). Clinical efficacy is achieved in NSCLC as a single agent and in CRC in combination with anti-EGFR monoclonal antibodies, however, secondary resistance impairs the effects of KRAS G12C blockade. In this work, we sought to determine the mechanisms of acquired resistance to concomitant KRAS-EGFR inhibition. In cell lines, patient-derived xenograft, and patient samples, a heterogeneous pattern of putative resistance alterations expected primarily to prevent inhibition of ERK signalling by drug can be detected at progression. Serial analysis of patient blood samples on treatment demonstrates that most of these alterations are detected at a low frequency that does not increase substantially and sometimes disappears over time, with the exception of KRAS G12C amplification which rises in step with tumour marker levels and clinical progression. Here we show that a CRC cell line that acquired resistance to sotorasib-cetuximab combination through KRAS G12C amplification became addicted to these agents and undergoes oncogene-induced senescence upon drug withdrawal. Accordingly, the KRAS G12C signal in circulating DNA from relapsed patients harbouring G12C amplification rapidly recedes upon treatment holiday. These data indicate that KRAS G12C amplification is a recurrent resistance mechanism to KRAS-EGFR co-inhibition and suggest a potential therapeutic vulnerability, whereby therapies that target this senescence response at drug withdrawal may overcome resistance to KRAS G12C-EGFR inhibition.
Possible mechanisms related to internal dynamics for anomalously high rainfall during August 2020 of Indian Summer Monsoon
Authors: Ganai Malay , Sarkar Sahadat , Kanase Radhika , Krishna R. Phani Murali , Mukhopadhyay P 
Published: January 2022

Abstract
In the present study, an investigation is made to understand the physical mechanism behind the anomalous high rainfall during August 2020 over the Indian subcontinent using both observation and GFS T1534 weather forecast model. According to India Meteorological Department (IMD), the country receives 27% excess rainfall in the month of August 2020. The excess rainfall is mainly contributed by the 5 well marked low pressure systems which formed over Bay of Bengal and moved west-northwestwards across central India up to Western Madhya Pradesh and Rajasthan. The analysis reveals that the observed anomalous rainfall is distributed over central India region extending from coastal Orissa to central part of Chhattisgarh, Madhya Pradesh and western coast of Gujarat region. It is also found that the August-2020 heavy rainfall is mainly contributed by the synoptic (2-10 days) component of the total rainfall whereas the contribution of the large-scale intraseasonal oscillation (ISO) component (10-90 days) is quite less. Although the present operational Global Forecast System (GFS) T1534 (GFS T1534) is able to predict the anomalous high rainfall with day-1 lead time, it underestimates the magnitude of the synoptic variance. Further, the large-scale dynamical and thermodynamical parameters show anomalous behaviour in terms of strong low level (850 hPa) jet, vertical velocity and associated moisture convergence in the lower level. The GFS T1534 is able to forecast the above large-scale features reasonably well even with day-5 lead time. From energetics analysis, it is found that the mean kinetic energy (MKE) is stronger for August 2020 as compared to climatological value and the strong MKE efficiently transfers the energy to the synoptic scale, and hence the synoptic eddy kinetic energy is higher. Along with that, the ISO scale kinetic energy for August 2020 is less compared to the August climatological value. GFS T1534 model has some fidelity in capturing the energy conversion processes, but it has some difficulty in capturing the magnitude with increased lead time.
Comprehensive Analysis of Retrotransposon Insertions within the Survival Motor Neuron Genes Involved in Spinal Muscular Atrophy
Authors: Pinto Albano , Cunha Catarina , Chaves Raquel , Butchbach Matthew ER , Adega Filomena 
Published: January 2022

Abstract
Transposable elements (TEs) are interspersed repetitive DNA sequences with the ability to mobilize in the genome. The recent development of improved tools for evaluating TE-derived sequences in genomic studies has enabled an increasing attention to the contribution of TEs to human development and disease. Spinal muscular atrophy (SMA) is an autosomal recessive motor neuron disease that is caused by deletions or mutations in the Survival Motor Neuron 1 (SMN1) gene. SMN2 gene is a nearly perfect duplication of SMN1. Both genes (collectively known as SMN1/SMN2) are highly enriched in TEs. A comprehensive analysis of TEs insertions in the SMN1/2 loci of SMA carriers, patients and healthy/control individuals was completed to perceive TE dynamics in SMN1/2 and try to establish a link between these elements and SMA.We found an Alu insertion in the promoter region and one L1 element in the 3’UTR that likely play an important role as an alternative promoter and as an alternative terminator to the gene, respectively. Additionally, the several Alu repeats inserted in the genes’ introns influence splicing, giving rise to alternative splicing events that cause RNA circularization and the birth of new alternative exons. These Alu repeats present throughout the genes are also prone to recombination events that can lead to SMN1 exons deletions, that ultimately lead to SMA. The many good and bad implications associated with the presence of TEs inside SMN1/2 make this genomic region ideal for understanding the implications of TEs on genomic evolution as well as on human genomic disease.