bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term intensive endurance exercise training is associated to reduced senescence markers in the colon mucosa of older adults
Authors: Demaria Marco , Bertozzi Beatrice , Veronese Nicola , Spelta Francesco , Cava Edda , Tosti Valeria , Piccio Laura , Early Dayna , Fontana Luigi 
Published: November 2022

AbstractRegular endurance exercise training is an effective intervention for the maintenance of metabolic health and the prevention of many age-associated chronic diseases. Several metabolic and inflammatory factors are involved in the health-promoting effects of exercise training, but regulatory mechanisms remain poorly understood. Cellular senescence—a cellular state of irreversible growth arrest—is considered a basic mechanism of aging. Senescent cells accumulate over time and promote a variety of age-related pathologies from neurodegenerative disorders to cancer. Whether long-term intensive exercise training affect the accumulation of age-associated cellular senescence is still unclear. Here, we show that the classical senescence markers p16 and IL-6 were markedly higher in the colon mucosa of middle-aged and older overweight adults than in young sedentary individuals, but that this upregulation was significantly blunted in age-matched endurance runners. Interestingly, we observe a linear correlation between the level of p16 and the triglycerides to HDL ratio, a well-accepted marker of colon adenoma risk and cardiometabolic dysfunctions. Our data suggest that chronic high-volume high-intensity endurance exercise can play a major role in preventing the accumulation of senescent cells in cancer prone tissues like colon mucosa with age. Future studies are warranted to elucidate which tissues are most affected, and what are the molecular and cellular mechanisms that mediate the senomorphic effects of different forms of exercise training.
Cytokine profiles and virological markers highlight distinctive immune statuses, and effectivenesses and limitations of antiviral therapies across different courses of chronic HBV infection
Authors: Xiao Lixin , Tang Kang , Fu Ting , Yuan Xiaojie , Seery Samuel , Zhang Weilu , Ji Zhaohua , He Zhen , Yang Yan , Zhang Wenhua , Jia Wenling , Liang Chunhui , Tang Haitao , Wang Fengmei , Ye Yancheng , Chen Lihua , Shao Zhongjun 
Published: November 2022

Abstract
Background
The characteristics of cytokine/chemokine (CK) profiles across different courses of chronic hepatitis B virus infection and the effects of antiviral therapies on cytokine profiles remain unclear.
Methods
This report provides evidence from 427 patients with chronic HBV infection. The Luminex multiple cytokine detection technology was used to detect CK profiles. The predictive power of CKs across different courses of chronic HBV infection was assessed using univariate analyses and with receiver operating characteristic (ROC) curves.
Results
Compared to chronic HBV carriers (CHC), expression levels of IL-21, IL-23, IL-33 and CXCL10 increased while IFN-γ did not change significantly in chronic hepatitis B (CHB). IL-8 increased in those with liver cirrhosis (LC). Whereas, myeloid-related markers decreased dramatically in those with hepatocellular carcinoma (HCC). The predictive results suggest that combining IL-6, IL-8, CXCL9 and CXCL11 into a nomogram could generate an accurate HCC risk based on LC status. In addition, the Spearman correlation coefficient suggested that CKs negatively correlate with serological and virological indicators, although they did positively correlate with one another. Antiviral treatments are capable of recovering normal liver functions and significantly reducing the viral load, however, they seem to have a limited effect in changing CKs, especially specific antiviral factors.
Conclusions
Variations in CKs and in virological markers might be used to determined distinct immune statuses. They might also highlight different levels of effectiveness and the limitations of antiviral treatments. This next step would to break new ground in the optimization of current anti-HBV treatment programs although this requires further research.
Comparison of the efficacy of anterior combined with posterior approach and posterior- only approach in the treatment of basilar invagination with irreducible atlantoaxial dislocation: minimum 5-year follow-up outcomes
Authors: Xu Tao , Cai Xiaoyu , Yasheng Paierhati , Yusufu Aierpati , Maimaiti Fulati , Maimaiti Maierdan , Guo Hailong , Sheng Jun , Deng Qiang , Sheng Weibin , Wang Ting 
Published: November 2022

Abstract
Background: To compare the clinical efficacy of anterior combined with posterior (A-P) approach and posterior-only (P-O) approach release, reduction, and internal fixation in the treatment of basilar invagination (BI) with irreducible atlantoaxial dislocation (AAD).
Methods: The clinical records of 49 patients diagnosed with BI with irreducible AAD from July 2000 to June 2017 were analyzed retrospectively. The patients were divided into the A-P group (n = 24) and P-O group (n = 25) according to the operation modes. The primary outcome measures included Chamberlain line (CL), Wackenheim line (WL), McRae line (ML), atlanto-dens interval (ADI), cervicomedullary angle (CMA), clivus-canal angle (CCA), Japanese Orthopaedic Association (JOA) score, and Ranawat grade.
Results: The follow-up periods were 68.7 ± 11.2 months in the A-P group and 64.4 ± 13.2 months in the P-O group. The JOA score of the A-P group was 8.25 ± 2.74 before operation and 14.96 ± 1.09 at the last follow-up. The improvement rate was 77.32 ± 11.31%. The JOA score of the P-O group was 8.10 ± 2.48 before operation and 14.69 ± 0.60 at the last follow-up. The improvement rate was 74.32 ± 10.42%. The preoperative CL, WL, ML, ADI, CMA, and CCA of the A-P group were 13.14 ± 5.72 mm, 6.91 ± 3.52 mm, 7.12 ± 4.51 mm, 9.78 ± 2.10 mm, 110.79°± 13.2°, 95.28° ± 18.1°. At the last follow-up, the CL, WL, ML, ADI, CMA, and CCA were 1.69 ± 2.51 mm, -2.71 ± 2.19 mm, -1.50 ± 2.41 mm, 1.13 ± 1.52 mm, 149.16° ± 12.5°, 141.38° ± 13.6° with significant difference compared with the preoperative results. The preoperative CL, WL, ML, ADI, CMA, and CCA of the P-O group were 12.50 ± 5.09 mm, 6.54 ± 3.12 mm, 6.88 ± 4.28 mm, 9.81 ± 1.89 mm, 115.29° ± 12.3°, 97.21° ± 16.6°. At the last follow-up, the CL, WL, ML, ADI, CMA, and CCA were 2.04 ± 3.61 mm, -3.12 ±1.81 mm, -1.72 ± 2.79 mm, 1.15 ± 1.20 mm, 146.69 °± 11.2 °, 137.49° ± 10.3°, which were statistically significant compared with the preoperative results. The bone graft fusion time was 9.1 ± 5.0 months in the A-P group and 9.4 ± 4.8 months in the P-O group. A total of 8 patients presented postoperative complications, including 6 patients in the A-P group (25.0%) and 2 patients in the P-O group (8.0%). The incidence of postoperative complications in the P-O was significantly lower than that in the A-P group.
Conclusion: The clinical efficacy of the P-O approach release, reduction, and internal fixation in the treatment of BI with irreducible AAD is basically the same as that of the A-P approach. However, the incidence of complications of the P-O approach is significantly lower than that of the A-P approach.
Risk factors for perioperative hidden blood loss after one-segment posterior circumferential decompression surgery on thoracic ossification of the posterior longitudinal ligament: a finding of the double-layer sign on CT
Authors: Liang Huiqiang , Zhao Xuan , Wang Linfeng , Li Jia , Shen Yong 
Published: November 2022

Abstract
Background: Hidden blood loss (HBL) is of increasing interest to spine surgeons. This retrospective study aimed to evaluate perioperative HBL and its risk factors in patients undergoing one-segment posterior circumferential decompression surgery on thoracic ossification of the posterior longitudinal ligament (T-OPLL).
Method: We retrospectively studied 112 patients diagnosed with T-OPLL following posterior circumferential decompression surgery from August 2015 to June 2020. Patient demographics, blood loss-related parameters, surgery-related data and imaging parameters were extracted. Postoperative complications were also recorded. Pearson or Spearman correlation analysis was used to investigate the correlation between patient demographics and HBL. Multivariate linear regression analysis was performed to determine the independent risk factors associated with HBL.
Results: Forty-five men and 67 women were involved in this research, with an average age of 56.4 ± 10.2 years. The mean HBL was 459.6±275.4 ml, accounting for 56.5% of the total blood loss. Multiple linear regression analysis showed that double-layer sign (P＝0.000), ossification occupancy ratio (OOR) >60%(P=0.030), age (P=0.010), hematocrit (Hct) loss (P=0.034), and postoperative Hct (P = 0.016) were independent risk factors for HBL. However, OPLL morphology (P=0.319), operation time (P=0.587), hemoglobin (Hb) loss (P=0.644), and postoperative Hb (P=0.952) were not significantly different from HBL.
Conclusion: A high proportion of HBL was found after posterior circumferential decompression surgery on T-OPLL during the perioperative period,which should not be overlooked. Double-layer sign, OOR> 60%, age, Hct loss and postoperative Hct are independent risk factors for HBL.
Pregnancy-induced differential expression of SARS-CoV-2 and influenza A viral entry factors in the lower respiratory tract
Authors: Giri Tusar , Panda Santosh , Palanisamy Arvind 
Published: November 2022

Abstract
Despite differences in the clinical presentation of coronavirus disease-19 and pandemic influenza in pregnancy, fundamental mechanistic insights are currently lacking because of the difficulty in recruiting critically ill pregnant subjects for research studies. Therefore, to better understand host-pathogen interaction during pregnancy, we performed a series of foundational experiments in pregnant rats at term gestation to assess the expression of host entry factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus (IAV) and genes associated with innate immune response in the lower respiratory tract. We report that pregnancy is characterized by a decrease in host factors mediating SARS-CoV-2 entry and an increase in host factors mediating IAV entry. Furthermore, using flow cytometric assessment of immune cell populations and immune provocation studies, we show an increased prevalence of plasmacytoid dendritic cells and a Type I interferon-biased environment in the lower respiratory tract of pregnancy, contrary to the expected immunological indolence. Our findings, therefore, suggest that the dissimilar clinical presentation of COVID-19 and pandemic influenza A in pregnancy could partly be due to differences in the extent of innate immune activation from altered viral tropism and indicate the need for comparative mechanistic investigations with live virus studies.
Quorum-quenching potential of recombinant PvdQ engineered bacteria for biofilm formation
Authors: Li Junlin , Li Zhifei , Xie Jun , Xia Yun , Gong Wangbao , Tian Jingjing , Zhang Kai , Yu Ermeng , Wang Guangjun 
Published: November 2022

Abstract
Quorum sensing (QS) is a core mechanism for bacteria to regulate biofilm formation, and therefore, QS inhibition or quorum quenching (QQ) is used as an effective and economically feasible strategy against biofilms. In this study, the PvdQ gene encoding AHL acylase was introduced into Escherichia coli (DE3), and a PvdQ-engineered bacterium with highly efficient QQ activity was obtained and used to inhibit biofilm formation. Gene sequencing and western blot analysis showed that the recombinant pET-PvdQ strain was successfully constructed. The color reaction of Agrobacterium tumefaciens A136 indicated that PvdQ engineering bacteria had shown strong AHL signal molecule quenching activity, and significantly inhibited the adhesion (motility) of Pseudomonas aeruginosa and biofilm formation of activated sludge bacteria in Membrane Bio-Reactor (MBR; inhibition rate 51–85%, p < 0.05). In addition, qRT-PCR testing revealed that recombinant PvdQ acylase significantly reduced the transcription level of QS biofilm formation related genes (cdrA, pqsA, and lasR; p < 0.05). In this study, QQ genetically engineered bacteria enhanced by genetic engineering could effectively inhibit the QS signal transduction mechanism and have the potential to control biofilm formation of pathogenic bacteria in the aquaculture environment, providing an environmentally friendly and alternative antibiotic strategy to suppress biofilm contamination.
Temporal changes of the oral and fecal microbiota after mild traumatic brain injury in rats by 16s rRNA sequencing
Authors: Wang Shujuan , Shang Yanjie , Pi Zhiyun , Zhou Ziqi , Zhang Xiangyan , Ren Lipin , Liang Weibo , Guo Yadong , Cai Jifeng , Zhang Changquan 
Published: November 2022

Abstract
Background
Mild traumatic brain injury (mTBI) could increase the risk of neurodegenerative-related diseases, and the serious long-term outcomes were often overlooked. The accurate identification of mTBI became fully urgent for the diagnosis and prevention of the long-term outcomes. In forensic science, the accurate identification of mTBI will directly affect the application of evidences in practice case. Recent researches have revealed that oral cavity and fecal microbiota played a fundamental role in deeply interconnecting gut and brain injury prevention and recovery by regulating immune homeostasis and neuroinflammation. Therefore, we investigated the relationship between the temporal changes of oral cavity and fecal bacterial communities and damage identification and post-injury time estimation after mTBI.
Results
In this study, we analyzed the oral cavity and fecal bacterial communities in mTBI rats under the 12 different post-injury times (sham, 0 h, 2 h, 6 h, 12 h, 24 h, 2 d, 3 d, 5 d, 7 d, 10 d, 14 d post-injury) using 16s rRNA sequencing technology. The sequence results revealed bacteria belonging to 36 phyla, 82 classes, 211 orders, 360 families, 751 genera, and 1398 species. Compared to the sham group, the relative abundance of the bacterial communities varied markedly in the post-injury groups.
Conclusions
Importantly, our data demonstrated that Fusobacteria, Prevotellaceae, Ruminococcaceae, and Lactobacillaceae might be the potential candidates for mTBI identification and the 2 h post-injury was a critical time point to explore the temporal changes of mTBI injury-time estimation. And the results could also provide new ideas for mTBI treatment in the clinic.
The supplementation of aquatic animal by-products hydrolysates can effectively improve the nutritional value of low fish meal feed for largemouth bass, Micropterus salmoides
Authors: Han Zhihao , Su Jilun , Xie Ruitao , Li Mingkong , Wang Zhenjie , Zhang Nihe , Chen Naisong , Li Songlin 
Published: November 2022

Abstract
The study was conducted to investigate the effects of aquatic animal by-products hydrolysates (AH) inclusion on the nutritive value of low-fishmeal diet for largemouth bass. The diet with 45% and 25% fishmeal was regarded as positive control (PC) and negative control (NC), respectively, and another three diets were prepared by the supplementation of shrimp hydrolysate powder (SHP) or by top-coating 1.5% of tuna visceral hydrolysate (TH) and shrimp by-product hydrolysate (SH) in the low fishmeal diet. Triplicate group of fish with initial body weight of 10.12 ± 0.02 g were fed one of the experimental diet twice daily for 62 days. The results of feeding trial revealed that the inclusion of AH, especially SHP, significantly alleviate the negative effects on growth performance caused by the low fishmeal diet. The beneficial role of AH supplementation partly related to the improved feed utilization, including FI, FER and PER. Although no significant difference was observed in the crude protein content of whole fish body, the inclusion of AH significantly decreased the low fishmeal diet caused the elevated moisture content. Meanwhile, the low fishmeal diet significantly decreased the crude lipid content of whole fish body, which was reversed by AH inclusion. The activity of serum lysozyme was significantly decreased by low fishmeal diet, but elevated with the inclusion of AH inclusion. Meanwhile, the inclusion of AH alleviated the low fishmeal diet induced the increased activity of AST. Additionally, the low fishmeal diet resulted in the decrease of total antioxidant capacity (T-AOC) and SOD activity, while the AH supplementation improved the negative effects on antioxidant capacity. No significant difference was observed in the expression of IGF-1 among treatments. The expression of TOR was significantly reduced by low fishmeal diet, and the AH inclusion elevated its expression. In conclusion, the inclusion of AH benefits for improving the nutritive value of low fishmeal diet for largemouth bass.
The influence of pH and H2O2 on surface quality and material removal rate during W-CMP
Authors: Wang Lin , Peng Feng , Chen Hongyu , Hang Wei , Yu Cuiping , Chen Shunhua , Zhao Shijun , Wu Zhenggang , Ma Yi , Lyu Binghai , Yuan Julong 
Published: November 2022

Abstract
In chemical mechanical polishing (CMP) process, chemical action is generally determined by pH regulator and oxidant in the polishing slurry. In this paper, tungsten was polished by CMP, and the material removal mechanism was discussed. The influence of pH values and H2O2 concentrations on surface quality and material removal rate (MRR) were studied deeply. The oxidation and dissolution kinetics of tungsten/tungsten oxide (W/WOx) during CMP process were investigated by electrochemical analysis and XPS. In addition, the microstructure of tungsten surface and its elemental composition and electronic state were also analyzed by SEM, white light interference and other characterization methods. Results reveal that tungsten has the best passivation effect at acidic pH, and the surface roughness increases with the increase of pH value, while the addition of H2O2 will deteriorate the surface quality of tungsten and improve the MRR. Besides, the different morphologies (corrosion pits, grain boundaries, etc.) formed after W-CMP indicate that the material removal mechanism is related to the composition of slurry.
KDM3B inhibitors disrupt PAX3-FOXO1 oncogenic activity in fusion positive rhabdomyosarcoma.
Authors: Kim Yong Yean , Gryder Berkley , Sinniah Ranuka , Peach Megan , Shern Jack , Abdelmaksoud Abdalla , Pomella Silvia , Woldemichael Girma , Stanton Benjamin , Milewski David , Barchi Joe , Schneekloth John , Chari Raj , Kowalczyk Joshua , Shenoy Shilpa , Evans Jason , Song Young , Wang Chaoyu , Wen Xinyu , Chou Hsien-chao , Gangalapudi Vineela , Esposito Dominic , Jones Jane , Procter Lauren , O’Neill Maura , Jenkins Lisa , Wei Jun , McMahon James , O'Keefe Barry , Hawley Robert , Khan Javed 
Published: November 2022

Abstract
Fusion-positive alveolar rhabdomyosarcoma (FP-RMS) is an aggressive pediatric sarcoma driven primarily by the PAX3-FOXO1 fusion oncogene, for which therapies targeting PAX3-FOXO1 are lacking. We screened 62,643 compounds using an engineered cell line that monitors PAX3-FOXO1 transcriptional activity identifying a hitherto uncharacterized compound, PFI-63. RNA-seq, ATAC-seq, and docking analyses implicated histone lysine demethylases (KDMs) as its targets. Enzymatic assays confirmed the inhibition of multiple KDMs with highest selectivity for KDM3B. Structural similarity search of PFI-63 identified PFI-90 with improved solubility and potency. Biophysical binding of PFI-90 to KDM3B was demonstrated using NMR and SPR. PFI-90 suppressed the growth of FP-RMS in vitro and in vivo through downregulating PAX3-FOXO1 activity, and combined knockdown of KDM3B and KDM1A phenocopied PFI-90 effects. Thus, we report novel KDM inhibitors with highest specificity for KDM3B. Its potent suppression of PAX3-FOXO1 activity can be exploited as a new therapeutic approach for FP-RMS and other transcriptionally driven cancers.
The use of artificial intelligence models to predict survival in patients with laryngeal squamous cell carcinoma
Authors: Choi Nayeon , Kim Junghyun , Yi Heejun , Kim HeeJung , Kim Tae Hwan , Chung Myung Jin , Ji Migyeong , Lee Sujeong , Kim Zero , Son Young-Ik 
Published: November 2022

Abstract
Most recent survival prediction has been based on TNM staging, which does not provide individualized information. However, clinical factors including performance status, age, sex, and smoking might influence survival. Therefore, we used artificial intelligence (AI) to analyze various clinical factors to precisely predict the survival of patients with larynx squamous cell carcinoma (LSCC). We included patients with LSCC (N=1026) who received definitive treatment from 2002 to 2020. Age, sex, smoking, alcohol consumption, Eastern Cooperative Oncology Group (ECOG) performance status, location of tumor, TNM stage, and treatment methods were analyzed using deep neural network (DNN) with multi-classification and regression, random survival forest (RSF), and Cox proportional hazards (COX-PH) model for prediction of overall survival. Each model was confirmed with five-fold cross validation, and performance was evaluated using linear slope, y-intercept, and C-index. The DNN with multi-classification model demonstrated the highest prediction power (1.000 ± 0.047, 0.126 ± 0.762, and 0.859 ± 0.018 for slope, y-intercept, and C-index, respectively), and the prediction survival curve showed the strongest agreement with the validation survival curve, followed by DNN with regression (0.731 ± 0.048, 9.659 ± 0.964, and 0.893 ± 0.017, respectively). The DNN model produced with only T/N staging showed the poorest survival prediction. When predicting the survival of LSCC patients, various clinical factors should be considered. In the present study, DNN with multi-class was shown to be an appropriate method for survival prediction. AI analysis may predict survival more accurately and improve oncologic outcomes.
The potential role of intestinal dysbacteriosis-related genes in hypertension: a bridge for future targeted therapies
Authors: wang kuangyi , jiang yufeng , Chen Tan , Zhou Yafeng 
Published: November 2022

Abstract
Background: Changes in the balance of intestinal flora can cause many diseases. This article offers a new direction to the future therapy of hypertension by screening potential drug targets for hypertension related to intestinal flora.
Method: Differential expression of GSE74144 in Gene Expression Omnibus (GEO) database was analyzed by GEO2R, grouping based on whether they had hypertension or not. Intestinal flora-related genes retrieved from the GeneCards database were intersected with the differential genes. The protein-protein interaction network was then constructed and the central gene was identified by Cytoscape. Candidate pharmacological compounds for differentially expressed genes were filtrated by cMap.
Results: We found high-expressed genes and 12 low-expressed genes were found in hypertension and intestinal microbiota related genes. These genes participate in biological processes including immune response regulation, bacterial defense response, glycolysis, viral response, etc. KEGG results show that these genes are related to inflammatory bowel disease, immune cell differentiation.
Conclusions: Through bioinformatics analysis, we have revealed the key genes related to intestinal flora in hypertension, as well as 10 drugs that may be used to treat hypertension, which provides a new way to treat hypertension in the future.