bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic retinal vessel analysis: flickering a light into the brain
Authors: Peterfi Anna , Pinaffi-Langley Ana Clara da C. , Szarvas Zsofia , Muranyi Mihaly , Kaposzta Zalan , Adams Cheryl , Pinto Camila Bonin , Mukli Peter , Kotliar Konstantin , Yabluchanskiy Andriy 
Published: January 2025

IntroductionGrowing aging populations pose new challenges to public health as the number of people living with dementia grows in tandem. To alleviate the burden of dementia, prodromal signs of cognitive impairment must be recognized and risk factors reduced. In this context, non-invasive techniques may be used to identify early changes and monitor disease progression. Dynamic retinal vessel analysis (DVA) provides an opportunity to measure retinal vasoreactivity in a way that may be comparable to cerebral vasoreactivity, thus providing a window to the brain.MethodsWe conducted a literature search on PubMed and Scopus to identify studies utilizing DVA to describe retinal vasoreactivity in central nervous system diseases and compare it with brain function and structure. We included original papers with full text in English.ResultsWe identified 11 studies, of which most employed a cross-sectional design (91%). Studies on cerebrovascular diseases reported that retinal vasoreactivity decreased in patient populations compared with that of healthy controls. Studies on cognitive impairment and dementia yielded mixed results, at least in part due to high population heterogeneity. There is also evidence for the association between DVA and brain and cognition parameters such as cerebral blood flow velocity, cerebral microvascular diffusivity, and cognitive function score.DiscussionThe reviewed papers on DVA and brain function, despite the mixed results, have demonstrated the relationship between retinal vasoreactivity and cerebrovascular function and cognition. Heterogeneity in study populations, procedures, and analyses make comparisons difficult. Studies with larger sample size, clear description of the population and methods, and standardized DVA analysis are needed to elucidate the eye–brain connection and to enhance the translational and clinical applications of DVA.
Influence of sedentary behavior on sleep quality in postmenopausal women in high-altitude regions of China: a cross-sectional study
Authors: Leng Rilang , Guo Ailin , Qian Guoping , Mao Sujie 
Published: January 2025

ObjectiveThis study investigates the association between sedentary behavior and sleep quality among postmenopausal women residing in China’s plateau regions. Particular attention is given to moderating effects of age, body mass index (BMI), and sleep environment. This study aims to identify modifiable risk factors influencing sleep quality in this high-altitude population.MethodsThis cross-sectional study focused on postmenopausal women (aged ≥50 years, ≥12 months post-menopause) across four primary plateau regions in China: Qinghai-Tibet, Yunnan-Guizhou, Inner Mongolia, and the Loess Plateau. Sedentary behavior was evaluated with the Older Adults Sedentary Behavior Questionnaire, and sleep quality was assessed with the Pittsburgh Sleep Quality Index. Data analysis encompassed descriptive statistics, correlation analysis, multiple linear regression, and subgroup analyses.ResultsAmong the 151 participants (mean age 58.5 years), sedentary behavior was positively correlated with poorer sleep quality (r = 0.36, p &lt; 0.001). Improvements in the sleep environment were similarly associated with better sleep quality (r = 0.29, p &lt; 0.001). Multiple linear regression identified sedentary behavior and sleep environment as significant predictors of sleep quality, while other variables showed no significant associations. Subgroup analysis revealed age-specific effects: sedentary behavior had a strong influence on sleep quality in women under 60 years (r = 0.36, p &lt; 0.01) but demonstrated a weaker, non-significant association in those aged 60 years or older (p = 0.062).ConclusionProlonged sedentary behavior is an independent risk factor for reduced sleep quality among postmenopausal women residing in high-altitude regions, while improvements in the sleep environment are positively associated with better sleep quality. The influence of sedentary behavior on sleep quality varies by age groups. These findings highlight the importance of tailored interventions and health policies to improving sleep quality in postmenopausal women living at high altitudes.
The potential impact of exercise on affect and neuroinflammation in older adults living with fibromyalgia: a scoping review
Authors: Taylor Taylor L. , Dodds Fitzgerald , Tharpe McKenna , Zumbro Emily L. , Hankes Michael , Jones Raymond , Rumble Deanna , Antoine Lisa , Allen-Watts Kristen , Sims Andrew , Chandra Reshu , Goodin Burel R. , Younger Jarred , Buford Thomas W. 
Published: January 2025

IntroductionFibromyalgia (FM) is a widespread chronic pain condition with prevalence increasing in older adults. Older adults living with FM experience longer pain symptom durations that can negatively impact their quality of life. Affect and neuroinflammation are potential factors that can exacerbate pain symptoms. Exercise is a recommended intervention to manage pain symptoms; however, adherence limitations persist. Drawing on the Biopsychosocial Framework of Chronic Pain, this scoping review explores how exercise impacts factors related to neuroinflammation and affect, and how these factors contribute to exercise adherence in older adults living with FM.MethodsWe conducted a scoping search of articles related to exercise and older adults living with FM published before 2024. The extracted study characteristics include publication type, study design, affect outcomes, neuroinflammation outcomes, exercise type, exercise adherence, and sample demographic information.ResultsWe have provided an overview of the relationship between affect and neuroinflammation in studies including older adults living with FM and highlight the impact of exercise on affect and neuroinflammation in older adults living with FM. A conceptual framework is provided illustrating the reciprocal relationship between exercise, affective changes, neuroinflammation, and exercise adherence.DiscussionOur results suggest that exercise may improve affect, while limited evidence suggests that aerobic and resistance exercise improve neuroinflammation. Finally, implications for importance and future directions in the context of potential biological factors impacted are provided.
Baseline dependent differences in the perception of changes in visuomotor delay
Authors: Vigh Gesche , Limanowski Jakub 
Published: January 2025

IntroductionThe detection of, and adaptation to delayed visual movement feedback has been extensively studied. One important open question is whether the Weber-Fechner Laws hold in the domain of visuomotor delay; i.e., whether the perception of changes in visuomotor delay depends on the amount of delay already present during movement.MethodsTo address this, we developed a virtual reality based, continuous hand movement task, during which participants had to detect changes in visuomotor mapping (delay): Participants (N = 40) performed continuous, auditory-paced grasping movements, which were measured with a data glove and transmitted to a virtual hand model. The movements of the virtual hand were delayed between 0 and 700 ms with the delay changing repeatedly in a roving oddball design. Participants had to indicate any perceived delay changes by key press. This design allowed us to investigate detection accuracy and speed related to the magnitude of the delay change, and to the “baseline” delay present during movement, respectively.ResultsAs expected, larger delay changes were detected more accurately than smaller ones. Surprisingly, delay changes were detected more accurately and faster when participants moved under large &gt; small delays.DiscussionThese results suggest that visual movement feedback delay indeed affects the detection of changes in visuomotor delay, but not as predicted by the Weber-Fechner Laws. Instead, bodily action under small delays may have entailed a larger tolerance for delay changes due to embodiment-related intersensory conflict attenuation; whereas better change detection at large delays may have resulted from their (visual) saliency due to a strong violation of visuomotor predictions.
Barriers and enablers in the micronutrient powder supply chain: lessons from a process evaluation of a home fortification programme in Bangladesh
Authors: Uddin Md. Fakhar , Islam Md. Aminul , Rahman Mahfuzur , Ahmed Tahmeed , Sarma Haribondhu 
Published: January 2025

BackgroundBangladesh Rural Advancement Committee (BRAC), a leading non-governmental organization (NGO), implemented a large-scale Home Fortification (HF) with Micronutrient Powder (MNP) programme from 2013 to 2018 aimed to reduce undernutrition and iron deficiency anemia among children aged below 5 years old. An adequate and timely supply of MNP was crucial for successful implementation of the programme, but very few studies have documented implementers’ MNP supply chain experiences. Therefore, this study aimed to explore the barriers and enablers in the MNP supply chain in Bangladesh.MethodsWe conducted this process evaluation in five rural sub-districts and three urban slums from March 2016 to February 2017. We conducted 15 Key Informant Interviews (KIIs) with HF programme personnel, 41 In-depth Interviews (IDIs) with direct HF programme implementers and reviewed relevant documents. We analyzed data using thematic and root-cause analysis approaches.ResultsParticipants reported the barriers in the MNP supply chain included lack of raw materials for MNP production by local manufacturer, political unrest and insufficient transport facilities, a lack of space for MNP buffer stock at BRAC’s central warehouse, and coordination gaps between BRAC’s national and sub-national level staff. Enablers to each of the barriers mentioned include ensuring buffer stock at all levels, raising separate transport requisition for MNP supply, and recruiting dedicated supply chain officers.ConclusionConcurrent course-corrections based on process evaluation findings improved MNP supply chain performance, resulting in higher MNP sales and coverage. The identified barriers and enablers provide useful insights for similar programs, emphasizing the importance of a resilient and well-managed MNP supply chain.
Association between the healthy eating index 2020 and heart failure among the U.S. middle-aged and older adults from NHANES 2005–2020: a cross-sectional study
Authors: Gu Fangfang , Yu Weiwei , Shu Tian , Zhu Yingwei 
Published: January 2025

ObjectiveThis study aims to shed light on the correlation between Healthy Eating Index-2020 (HEI-2020) and heart failure (HF) in American adults aged 50 or above.MethodsData were from the National Health and Nutrition Examination Survey 2005–2020, encompassing 13,105 participants with an age of 50 or above. HEI-2020 score was utilized for rating the dietary quality. The link of HEI-2020 to HF was assessed via logistic regression, restricted cubic splines (RCS), generalized additive models (GAM), weighted quantile sum (WQS) regression, as well as quantile g-computation (Qgcomp) models.ResultsA negative association between HEI-2020 and HF risk was uncovered in middle-aged and older Americans (OR = 0.99, 95% CI: 0.98–1.00, p = 0.006). The highest quartile (Q4) exhibited a markedly lower HF risk than the lowest quartile (Q1) (OR = 0.70, 95% CI: 0.55–0.89, p = 0.004). RCS and GAM analyses demonstrated a linear dose–response relationship between HEI-2020 and HF. Finally, WQS regression and Qgcomp models revealed a beneficial combined influence of 13 dietary components on HF risk, with dairy and whole fruits emerging as the most influential.ConclusionElevated HEI-2020 scores are linked to decreased HF risks among Americans aged 50 or above, suggesting that adherence to the Dietary Guidelines for Americans can mitigate HF risk.
Association between serum carotenoids levels and severe headache or migraine in adults: a cross-sectional study from NHANES
Authors: Hu Tian , Chen Yufei , Chen Siyu , Xue Rui 
Published: January 2025

BackgroundCarotenoids are well-established for their potent antioxidant properties; however, their potential association with severe headaches or migraines remains largely unexamined. This study was conducted to explore the relationship between serum carotenoid levels and the prevalence of severe headaches or migraines within the US population.MethodsWe utilized data from the 2001–2004 National Health and Nutrition Examination Survey (NHANES), which comprised a total of 8,910 participants. Serum carotenoid levels—specifically α-carotene, β-carotene, β-cryptoxanthin, lutein/zeaxanthin, and lycopene—were quantified using high-performance liquid chromatography. Migraine status was determined based on a questionnaire. The research methodologies employed included multivariate logistic regression, subgroup analysis, and restricted cubic spline (RCS) models.ResultsThe prevalence of migraines in the study population was 22.37%. Multivariate logistic regression analysis indicated that serum concentrations of α-carotene (OR = 0.91, 95% CI: 0.85–0.97), β-carotene (OR = 0.88, 95% CI: 0.81–0.94), β-cryptoxanthin (OR = 0.83, 95% CI: 0.76–0.90), lutein/zeaxanthin (OR = 0.75, 95% CI: 0.67–0.85), and total carotenoids (OR = 0.79, 95% CI: 0.70–0.90) were significantly inversely correlated with severe headaches or migraines; however, no significant association was found for lycopene levels. RCS analysis showed that β-cryptoxanthin had an L-shaped non-linear relationship with migraine prevalence at a threshold of approximately 9.392 μg/dL, while subgroup analyses confirmed the consistent inverse association between total serum carotenoid concentrations and migraine prevalence across various groups.ConclusionSerum concentrations of α-carotene, β-carotene, β-cryptoxanthin, lutein/zeaxanthin, and total serum carotenoids were inversely correlated with the incidence of severe headaches or migraines in US adults. This evidence indicates that carotenoids may provide a protective effect against migraines; however, further investigation is warranted to substantiate these associations and to elucidate the underlying mechanisms.
Application of dynamic enhanced scanning with GD-EOB-DTPA MRI based on deep learning algorithm for lesion diagnosis in liver cancer patients
Authors: Liu Bo , Yang Jinhua , Wu Yifei , Chen Xi , Wu Xueru 
Published: January 2025

BackgroundImprovements in the clinical diagnostic use of magnetic resonance imaging (MRI) for the identification of liver disorders have been made possible by gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA). Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) technology is in high demand.ObjectivesThe purpose of the study is to segment the liver using an enhanced multi-gradient deep convolution neural network (EMGDCNN) and to identify and categorize a localized liver lesion using a Gd-EOB-DTPA-enhanced MRI.MethodsWe provided the classifier images of the liver in five states (unenhanced, arterial, portal venous, equilibrium, and hepatobiliary) and labeled them with localized liver diseases (hepatocellular carcinoma, metastasis, hemangiomas, cysts, and scarring). The Shanghai Public Health Clinical Center ethics committee recruited 132 participants between August 2021 and February 2022. Fisher’s exact test analyses liver lesion Gd-EOB-DTPA-enhanced MRI data.ResultsOur method could identify and classify liver lesions at the same time. On average, 25 false positives and 0.6 real positives were found in the test instances. The percentage of correct answers was 0.790. AUC, sensitivity, and specificity evaluate the procedure. Our technique outperforms others in extensive testing.ConclusionEMGDCNN may identify and categorize a localized hepatic lesion in Gd-EOB-DTPA-enhanced MRI. We found that one network can detect and classify. Radiologists need higher detection capability.
Loss of SMAD1 in acute myeloid leukemia with KMT2A::AFF1 and KMT2A::MLLT3 fusion genes
Authors: Dietsche Lisa , Stirm Kristin , Lysenko Veronika , Schneidawind Corina , Tzankov Alexandar , Müller Anne , Theocharides Alexandre P. A. 
Published: January 2025

IntroductionKMT2A-rearrangements define a subclass of acute leukemias characterized by a distinct gene expression signature linked to the dysfunctional oncogenic fusion proteins arising from various chromosomal translocations involving the KMT2A (also known as MLL1) gene. Research on the disease pathomechanism in KMT2A-rearranged acute leukemias has mainly focused on the upregulation of the stemness-related genes of the HOX-family and their co-factor MEIS1.ResultsHere we report the KMT2A::AFF1 and KMT2A::MLLT3 fusion gene-dependent downregulation of SMAD1, a TGF-β signaling axis transcription factor. SMAD1 expression is lost in the majority of AML patient samples and cell lines containing the two fusion genes KMT2A::AFF1 and KMT2A::MLLT3 compared to non-rearranged controls. Loss of SMAD1 expression is inducible by introducing the respective two KMT2A fusion genes into hematopoietic stem and progenitor cells. The loss of SMAD1 correlated with a markedly reduced amount of H3K4me3 levels at the SMAD1 promoter in tested cells with KMT2A::AFF1 and KMT2A::MLLT3. The expression of SMAD1 in cells with KMT2A::AFF1 fusion genes impacted the growth of cells in vitro and influenced engraftment of the KMT2A::AFF1 cell line MV4-11 in vivo. In MV4-11 cells SMAD1 expression caused a downregulation of HOXA9 and MEIS1, which was reinforced by TGF-β stimulation. Moreover, in MV4-11 cells SMAD1 presence sensitized cells for TGF-β mediated G1-arrest.ConclusionOverall, our data contributes to the understanding of the role of TGF-β signaling in acute myeloid leukemia with KMT2A::AFF1 by showing that SMAD1 loss can influence the growth dynamics and contribute to the pathogenic expression of disease driving factors.
Genomic, transcriptomic, and T cell receptor profiling in stratifying response to first-line chemoradiotherapy or radiotherapy for esophageal squamous cell carcinoma
Authors: Zhang Xiaqin , Lian Jianhong , Chen Fukun , Wang Kai , Xue Haoyuan , Jia Sufang , Wang Weili , Li Zhongkang , Liang Hua , Li Hongwei 
Published: January 2025

IntroductionEsophageal squamous cell carcinoma (ESCC) accounts for 80% of esophageal cancer (EC) worldwide. The molecular characteristics of locally advanced ESCC have been extensively studied.MethodsIn this study, we investigate the genomic and transcriptomic characteristics and try to provide the basic T-cell receptors (TCRs) dynamics and its genomic and transcriptome association during the radiochemotherapy of ESCC using multi-omics analysis.ResultsA total of 23 patients with pathologic diagnoses of locally advanced ESCC were enrolled. The median tumor mutational burden (TMB) of the 23 ESCC patients were 3.47 mutations/ Mb (mega-base). The TP53, RTK/RAS, and NOTCH pathways were concurrently prevalent in ESCC. Besides, some less prevalent pathways, including WNT and HIPPO pathways also exhibited superior frequencies in ESCC. Meantime, we found the immune-hot tumor had higher immune infiltration scores. The median TMB in the progression-free survival (PFS) low group was significantly higher than that in the PFS-high group. The chromosomal copy number variation (CNV) burden of the neutrophil-to-lymphocyte ratio (NLR)-high group appeared to be higher than that of the NLR-low group, and the StromalScore in the NLR-low group was significantly higher. Clonality score was significantly increased from pre-treat to post-treat and from on-treat to post-treat. Shannon index was significantly decreased from pre-treat to post-treat and from on-treat to posttreat. Richness was significantly decreased from pre-treat to post-treat.DiscussionMultiomics analysis provided the basic TCRs dynamics and their genomic and transcriptome association during the radio-chemotherapy of 23 locally advanced ESCC in China, and provided a valuable insights into the heterogeneity and the tumor microenvironment and treatment responses. Meantimes, the identification of biomarkers and the exploration of their association with treatment outcomes could have important implications for clinical practice.
Early recurrence after surgery in FIGO 2023 stage I-III endometrial cancer: characteristics and risk factors
Authors: Huang Zhen , Li Xue , Li Ling 
Published: January 2025

BackgroundUnderstanding the risk factors for early recurrence is crucial for improving endometrial cancer (EC) patient outcomes.MethodsWe conducted a retrospective analysis of clinicopathological data from 473 patients diagnosed with EC at the First Affiliated Hospital of Chongqing Medical University between October 2013 and May 2019. We evaluated factors influencing early recurrence(defined as occurring within 12 months after treatment) based on 2023 International Federation of Gynecology and Obstetrics (FIGO) staging system.ResultsAmong the 473 patients, 284 (60.1%) were diagnosed with stage I, 117 (24.7%) with stage II, and 72 (15.2%) with stage III. A total of 343 patients (72.5%) had non-aggressive EC, while 130 patients (27.5%) had aggressive EC. Our findings identified higher FIGO stage, lymphovascular space invasion, estrogen receptor negativity, and abnormal P53 expression as significant independent risk factors for early recurrence. Of the 473 patients, 83 (17.6%) experienced recurrence, with 44 patients (53.0%) relapsing within 12 months post-treatment. Patients with early recurrence had significantly worse prognoses compared to those with late recurrence or no recurrence(P &lt; 0.001).ConclusionThe identification of these risk factors is essential for developing individualized treatment plans and postoperative management strategies. Our study highlights the need for targeted therapies and intensified follow-up for high-risk patients to improve outcomes in endometrial cancer.
Exploration of ANKRD27 as an immune-related prognostic factor in pan-cancer and hepatocellular carcinoma
Authors: Shen Ningzhe , Fan Congcong , Ying Haosun , Li Xinmiao , Zhang Weizhi , Yu Jinglu , Zheng Jianjian , Li Yifei 
Published: January 2025

IntroductionAnkyrin repeat domain 27 (ANKRD27) has been found to be associated with certain cancers. However, its clinical potential in pan-cancer remains unclear.MethodsPublic datasets (TCGA and GTEx) were applied to analyze ANKRD27 expression in multiple cancer types and its correlations with immune scores, immune checkpoint genes, and immune modulatory genes. We also examined ANKRD27 expression in hepatocellular carcinoma (HCC) patients using TCGA and GSE14520 datasets. The upregulation of ANKRD27 was verified via qRT-PCR in vitro. Based on TCGA-HCC, external, and GSE14520 cohorts, the associations between ANKRD27 expression and survival outcome were explored via the Kaplan-Meier survival curve. The effects of ANKRD27 reduction on HCC cell growth, movement, and invasion were evaluated by CCK-8, Wound healing, and Transwell assays.ResultsANKRD27 exhibited aberrant expression in multiple cancers and was correlated with immune traits, including immune infiltration, immune checkpoint genes, and immune modulatory genes. Elevated expression of ANKRD27 was found in TCGA-HCC and GSE14520 cohorts and was confirmed in HCC cell lines. HCC patients with high ANKRD27 expression had poorer prognosis. In vitro, reducing ANKRD27 decreased the capability of proliferation, migration, and invasion in HCC cells. High ANKRD27 expression was associated with sensitivity to certain drugs.ConclusionANKRD27 displays abnormal levels of expression in different cancer types and is linked to immune status in cancer. Furthermore, ANKRD27 may serve as a prognostic predictor for HCC.