bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gender voices in Afghanistan primary and secondary school English textbooks
Authors: Orfan Sayeed Naqibullah 
Published: September 2022

AbstractTextbooks are one of the areas in which gender inequality is institutionalized effortlessly. The study investigated the representation of gender in primary and secondary English textbooks. A total of 14 categories of underrepresentation and misrepresentation were used to examine gender portrayal. The results show that women are significantly underrepresented whilst men are overrepresented in text and illustrations. Female names account for only 30.4% of names in textbooks. Male characters precede female characters in most coordinated phrases, and most of the dialogues are initiated by men. Masculine generic forms are used to refer to women and men including in the English translation of the Afghanistan National Anthem. Men are more frequently portrayed in a myriad of social roles than women are. Surprisingly, this pattern also holds true for domestic roles. Furthermore, little space is dedicated to female famous persons (e.g., scientists), and women constitute a tiny fraction of the main characters of articles and stories, most of which were about trivial matters (e.g., cooking). They are portrayed in a small number of activities, almost a quarter of which are house‐related tasks.
Early childhood stress responses to psychosocial stressors: The state of the science
Authors: Bates Randi A. , Militello Lisa , Barker Erin , Villasanti Hugo Gonzalez , Schmeer Kammi 
Published: September 2022

AbstractThe aim of this systematic review was to better understand whether and to what extent psychosocial stressors are associated with hypothalamic–pituitary–adrenal axis or autonomic nervous system stress responses in young children (1–6 years of age). Studies were classified by psychosocial stressors from the ecobiodevelopmental model: social and economic resources, maternal mental health, parent–child relationships, and the physical environment. Of the 2388 identified studies, 32 met full inclusion criteria, including over 9107 children. Child physiologic stress responses were measured as hair and urinary cortisol and cortisone, salivary diurnal and reactive cortisol, salivary reactive alpha‐amylase, and respiratory sinus arrhythmia. There were 107 identified relations between psychosocial stressors and physiologic stress responses. Nearly two thirds of these relations suggested that children have dysregulated stress responses as either significantly blunted (n = 27) or increased (n = 37); 43 relations were not significant. Children most consistently had significantly dysregulated stress responses if they experienced postnatal maternal depression or anxiety. Some reasons for the mixed findings may be related to characteristics of the child (i.e., moderators) or stressor, how the stress response or psychosocial stressor was measured, unmeasured variables (e.g., caregiving buffering), researcher degrees of freedom, or publication bias.
Groebke‐Blackburn‐Bienaymé Multicomponent Reaction Catalysed by Reusable Brønsted‐Acidic Ionic Liquids
Authors: Anjos Nicolas S. , Chapina Agáta I. , Santos Ana R. , Licence Peter , Longo Luiz S. 
Published: September 2022

AbstractIn the present work, the use of Brønsted acidic ionic liquids (BAIL) as catalysts for Groebke‐Blackburn‐Bienaymé (GBB) multicomponent reactions was systematically investigated. A series of four 1‐(butyl‐4‐sulfonic)‐3‐methylimidazolium salts bearing different anions was easily prepared and screened as acidic catalysts for this transformation. The best reaction conditions were stablished as 20 mol % of catalyst [(SO3H)4C4C1Im][OTf] in refluxing EtOH or MeOH under conventional heating and in sealed tube at 150 °C for 1–4 hours under microwave heating, affording a series of imidazo‐fused heterocycles in moderate to excellent yields (42–93 %). The homogeneous BAIL catalyst could be recycled and reused in four consecutive reaction cycles, despite of a laborious recovery procedure employed. This approach represents the first example of a task‐specific reusable homogeneous Brønsted acidic catalyst in GBB reactions and opens new opportunities for the exploration of acidic ionic liquid phases as catalysts for this fascinating multicomponent reaction.
Second Generation Self‐Assembling Dendrimer Combining Supramolecular and Dynamic Covalent Chemistry
Authors: Wetzel Darte , Lüning Ulrich 
Published: September 2022

AbstractA monodisperse second generation self‐assembled dendrimer has been obtained combining supramolecular chemistry and dynamic covalent chemistry as orthogonal binding moieties. A branching unit 18 and a core 5 were connected via a heteroleptic terpyridine zinc complex exploiting the sterical hindrance of mesitylene units in the 6,6’’‐position of one of the terpyridines as the distinction between the ligands. Solubility problems could be solved by introduction of an ethylhexyl ether. The final sphere of the dendrimer was connected via imines, hydrazones and oximes, whereby the oxime made from O‐methylhydroxylamine (22) led to a complete turnover of all aldehyde functions. Furthermore, no amine signal was left and also no signals for a diffusion constant of the hydroxylamine 22 were observed. The successful formation of the dendrimers was analyzed by 1H NMR and DOSY NMR spectroscopy. Furthermore both, the first and second generation dendrimers could be detected by ESI HRMS.
A database for sea‐level monitoring in French Polynesia
Authors: Barriot Jean‐Pierre , Zhang Fangzhao , Ducarme Bernard , Wöppelmann Guy , André Gaël , Gabillon Alban 
Published: September 2022

AbstractThis article presents a curated database of the sea‐level measurements acquired by the network of the five geodetic tide gauges managed over French Polynesia by the Geodesy Observatory of Tahiti from 13 June 2009 to 28 January 2021. A unique feature of this database, with respect to previous databases that host the same raw data, like the Intergovernmental Oceanographic Commission database (IOC, www.ioc‐sealevelmonitoring.org) and the database of ‘Réseaux de référence des observations marégraphiques’ (REFMAR, http://refmar.shom.fr) is that all the time‐tags of the raw measurements (1‐ or 2‐min sampling) have been validated and, if necessary, corrected with a precision of 2 min (time shifts of up to 1 hr can be present in the raw data). Possible outliers have also been flagged, but not removed. In addition, smoothed hourly data are also provided, along with tidal analysis results and estimations of the sea‐level trends for the five tide gauges, with respect to their local geodetic datum. The database, entitled ‘SEA LEVEL collected from TIDE STATIONS in South Pacific Ocean from 2009‐06‐13 to 2021‐01‐28’, can be accessed on the NOAA data servers as ‘NCEI Accession 0244182’ and contains two subsets: The first one is relative to the original sampling rate and the second one is relative to an hourly re‐sampling with harmonic tide models for each tide gauge station.
Gray and white matter astrocytes differ in basal metabolism but respond similarly to neuronal activity
Authors: Köhler Susanne , Winkler Ulrike , Junge Tabea , Lippmann Kristina , Eilers Jens , Hirrlinger Johannes 
Published: September 2022

AbstractAstrocytes are a heterogeneous population of glial cells in the brain, which adapt their properties to the requirements of the local environment. Two major groups of astrocytes are protoplasmic astrocytes residing in gray matter as well as fibrous astrocytes of white matter. Here, we compared the energy metabolism of astrocytes in the cortex and corpus callosum as representative gray matter and white matter regions, in acute brain slices taking advantage of genetically encoded fluorescent nanosensors for the NADH/NAD+ redox ratio and for ATP. Astrocytes of the corpus callosum presented a more reduced basal NADH/NAD+ redox ratio, and a lower cytosolic concentration of ATP compared to cortical astrocytes. In cortical astrocytes, the neurotransmitter glutamate and increased extracellular concentrations of K+, typical correlates of neuronal activity, induced a more reduced NADH/NAD+ redox ratio. While application of glutamate decreased [ATP], K+ as well as the combination of glutamate and K+ resulted in an increase of ATP levels. Strikingly, a very similar regulation of metabolism by K+ and glutamate was observed in astrocytes in the corpus callosum. Finally, strong intrinsic neuronal activity provoked by application of bicuculline and withdrawal of Mg2+ caused a shift of the NADH/NAD+ redox ratio to a more reduced state as well as a slight reduction of [ATP] in gray and white matter astrocytes. In summary, the metabolism of astrocytes in cortex and corpus callosum shows distinct basal properties, but qualitatively similar responses to neuronal activity, probably reflecting the different environment and requirements of these brain regions.
Single‐cell transcriptomics uncovers cellular architecture and developmental trajectories in hepatoblastoma
Authors: Huang Hongting , Wu Liang , Lu Li , Zhang Zijie , Qiu Bijun , Mo Jialin , Luo Yi , Xi Zhifeng , Feng Mingxuan , Wan Ping , Zhu Jianjun , Yu Dingye , Wu Wei , Tan Kezhe , Liu Jiangbin , Sheng Qingfeng , Xu Ting , Huang Jinyan , Lv Zhibao , Tang Yujie , Xia Qiang 
Published: September 2022

Background and Aims:
Hepatoblastoma (HB) is the predominant type of childhood liver cancer. Treatment options for the clinically advanced HB remain limited. We aimed to dissect the cellular and molecular basis underlying HB oncogenesis and heterogeneity at the single‐cell level, which could facilitate a better understanding of HB at both the biological and clinical levels.

Approach and Results:
Single‐cell transcriptome profiling of tumor and paired distal liver tissue samples from five patients with HB was performed. Deconvolution analysis was used for integrating the single‐cell transcriptomic profiles with the bulk transcriptomes of our HB cohort of post–neoadjuvant chemotherapy tumor samples. A single‐cell transcriptomic landscape of early human liver parenchymal development was established for exploring the cellular root and hierarchy of HB oncogenesis. As a result, seven distinct tumor cell subpopulations were annotated, and an effective HB subtyping method was established based on their compositions. A HB tumor cell hierarchy was further revealed to not only fit with the classical cancer stem cell (CSC) model but also mirror the early human liver parenchymal development. Moreover, FACT inhibition, which could disrupt the oncogenic positive feedback loop between MYC and SSRP1 in HB, was identified as a promising epigenetic‐targeted therapeutic strategy against the CSC‐like HB1–Pro‐like1 subpopulation and its related high‐risk “Pro‐like1” subtype of HB.

Conclusions:
Our findings illustrate the cellular architecture and developmental trajectories of HB via integrative bulk and single‐cell transcriptome analyses, thus establishing a resourceful framework for the development of targeted diagnostics and therapeutics in the future.
Combination of Doppler measurements with amniotic fluid volume for the prediction of perinatal outcomes in fetal growth restriction
Authors: Besimoglu Berhan , Uyan Hendem Derya , Atalay Ayşegül , Göncü Ayhan Şule , Sınacı Selcan , Tanaçan Atakan , Şahin Dilek 
Published: September 2022

AbstractObjectivesWe aimed to evaluate a new ratio of amniotic fluid volume (AFV) to Doppler measurements, and compare its association with adverse perinatal outcomes (APOs) in fetal growth restriction (FGR).MethodsThis prospective study included pregnant women with singleton fetal growth–restricted fetuses. Each participant underwent a detailed ultrasonographic examination. Uterine artery pulsatility index, umbilical artery pulsatility index (UA PI), middle cerebral artery pulsatility index (MCA PI), cerebroplacental ratio (CPR), and umbilical‐to‐cerebral ratio (UCR) were calculated, and the single deepest pocket (SDP) technique was used to estimate AFV. Amniotic‐umbilical‐to‐cerebral ratio (AUCR) was calculated as the ratio of SDP to UCR: AUCR = SDP / (UA PI/MCA PI). APO was defined as umbilical venous cord blood pH <7.10, 5‐minute APGAR score <7, and neonatal intensive care unit admission.ResultsWe compared the fetal ultrasonographic and demographic features between the APO and the non‐APO groups. The mean UA PI and UCR were significantly higher in the APO group. The mean SDP, CPR, and AUCR were significantly lower in the APO group. Receiver operating characteristic curve analyses demonstrated the highest area under the curve value (0.882; P < 0.001) for AUCR to APOs.ConclusionThe current study suggests that AUCR is the best predictor for APOs in FGR.
Way of love and after‐marriage well‐being: Evidence from China
Authors: Li Bowen , Zhou Cai , Luo Ji 
Published: September 2022

AbstractBased on 2010 China Family Panel Studies, we use Ordered Probit, Instrumental Variables, and Conditional Mixed Process to analyze the effect of different ways of love on after‐marriage well‐being. The findings are: (i) Compared with arranged marriage, free love can significantly increase the well‐being of married residents. (ii) In heterogeneity analysis, the promotion effect of free love on well‐being is more obvious among the couples 2 years after marriage, residents with positive personal values, the post‐80s, and rural areas. (iii) In mechanism analysis, married residents who met through free love will significantly enhance their ability to get along with others, increase their intimacy with their spouses, improve family harmony, and reduce their emphasis on family succession, thus further enhancing their well‐being.
Too smoggy for kids to go to school? Air quality and preschool enrollments in Chinese cities
Authors: Zhang Wei 
Published: September 2022

AbstractEducation outcomes of children may be influenced by many factors, including exposure to air pollution. This study examines whether air quality affects school enrollments. Different from the contemporaneous consequences of exposure to pollution, school enrollments are annual decisions and arguably more correlated with human capital accumulation than absences or exam scores. Our estimates suggest that increases in the number of days with low visibility are associated with reductions in preschool enrollments. Keeping children from enrolling in preschools is a pollution avoidance behavior currently undocumented in the literature. Our findings are robust to alternative enrollment and air quality measures.
Competing farm programs: Does the introduction of a risk management program reduce the enrollment in the Conservation Reserve Program?
Authors: Yu Jisang , Goodrich Brittney , Graven Atticus 
Published: September 2022

AbstractGovernment‐supported farm programs can provide farm operators with different economic incentives regarding the use of their land. We study two US farm programs relevant to hay and pastureland, the Conservation Reserve Program (CRP) and Pasture, Rangeland, and Forage (PRF) Index Insurance. Using county‐level enrollment and availability data, we find that the introduction of PRF reduces enrollment in CRP. This interaction potentially returns environmentally sensitive farmland back into production. We also document the heterogeneity of the effects over time and the dynamic effects across the length of exposure.
Importance of diet in amphibian metamorphosis‐based studies designed to assess the risk of thyroid active substances
Authors: Fort Douglas J. , Leopold M. Annegaaike , Wolf Jeffrey C. , Todhunter Kevin J. , Weterings Peter J. J. M. 
Published: September 2022

AbstractThe present study evaluated the hypothesis that dietary quality used in historical studies may impact the effects of chemical stressors on premetamorphic development and metamorphosis due to suboptimal nutritional quality. A modified Amphibian Metamorphosis Assay (AMA) was performed in which Nieuwkoop and Faber (NF) Stage 47 tadpoles of Xenopus laevis were exposed for 32 days to iodide (I−)‐deficient FETAX solution supplemented with <0.025, 0.17, 0.52, 1.58, and 4.80 μg I−/L (measured concentrations 0.061, 0.220, 0.614, 1.65, and 4.73 μg I−/L) and fed a pureed Frog Brittle (FB) diet. An AMA guideline benchmark group (four replicates) exposed to dechlorinated tap water and fed standard Sera Micron Nature® (SMN) diet was evaluated concurrently. Developmental delay, observed as changes in stage distribution or median developmental stage, occurred in FB treatments with 0.061, 0.220, and 0.614 μg/L I−, respectively. Developmental rates and hind limb length of the 1.65 and 4.73 μg/L I− groups were similar to each other, but both treatments fell short of the developmental rate achieved by the SMN benchmark. Iodide supplementation also had no impact on nonthyroidal growth endpoints, which were markedly reduced in FB‐fed frogs compared with their SMN‐fed counterparts. All larvae that received the FB diet had mildly to severely hypoplastic/atrophic thyroids, a condition for which iodine supplementation had little if any ameliorative effect. Collectively, these results suggested that nutritional deficiencies in the FB diet negatively affected both growth and metamorphic development, the latter of which was only compensated to a limited extent by iodine supplementation.
Imaging Parhyale hawaiensis embryogenesis with frequency domain photoacoustic microscopy: A novel tool in developmental biology
Authors: Tserevelakis George J. , Velentza Sofia , Liaskas Ioannis , Archontidis Themis , Pavlopoulos Anastasios , Zacharakis Giannis 
Published: September 2022

AbstractWe present the application of a low‐cost frequency domain photoacoustic (FDPA) microscope for the label‐free imaging of live developing embryos of the crustacean model organism Parhyale hawaiensis. By modulating the intensity of a continuous wave laser source at 9.5 MHz, we achieve the excitation of monochromatic PA waves, which are detected to provide amplitude and phase recordings. The data are subsequently processed to generate accurate maximum amplitude projection and surface reconstructions, delineating the morphological features of the embryos with high resolution and contrast. The findings of this study pave the way for the broader adoption of inexpensive PA diagnostic techniques in developmental biology, shedding light on various fundamental processes in established and emerging model organisms.
Design of new hydrolyzed collagen‐modified magnetic nanoparticles to capture pathogens
Authors: Sande Maria G. , Roque Lúcia , Braga Adelaide , Marques Márcia , Ferreira Débora , Saragliadis Athanasios , Rodrigues Joana L. , Linke Dirk , Ramada David , Silva Carla , Rodrigues Lígia R. 
Published: September 2022

AbstractEnrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen‐binding adhesins with hydrolyzed collagen‐coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time needed to confirm the presence of a bacterial infection. MNPs were synthesized by a simple two‐step approach through a green co‐precipitation method using water as solvent. These MNPs were specifically designed to interact with pathogenic bacteria by establishing a hydrolyzed collagen‐adhesin linker. The bacterial capture efficacy of hydrolyzed collagen MNPs (H‐Coll@MNPs) for bacteria expressing collagen binding adhesins was 1.3 times higher than that of arginine MNPs (Arg@MNPs), herein used as control. More importantly, after optimization of the MNP concentration and contact time, the H‐Coll@MNPs were able to capture 95% of bacteria present in the samples. More importantly, the bacteria can be enriched within 30 min and the time for bacterial identification is effectively shortened in comparison to the “gold standard” in clinical diagnosis. These results suggest that H‐Coll@MNPs can be used for the selective isolation of specific bacteria from mixed populations present, for example, in biological samples.
The Fractalkine‐CX3CR1 Axis Regulates Non‐inflammatory Osteoclastogenesis by Enhancing Precursor Cell Survival
Authors: Kuboi Yoshikazu , Kuroda Yukiko , Ohkuro Masayoshi , Motoi Sotaro , Tomimori Yoshiya , Yasuda Hisataka , Yasuda Nobuyuki , Imai Toshio , Matsuo Koichi 
Published: September 2022

ABSTRACTThe chemokine fractalkine (FKN) is produced by various cell types, including osteoblasts and endothelial cells in bone tissue, and signals through a sole receptor, CX3CR1, which is expressed on monocytes/macrophages, including osteoclast precursors (OCPs). However, the direct effects of FKN signaling on osteoclast lineage cells under homeostatic noninflammatory conditions remain unclear. Here, we report that FKN regulates mouse OCP survival and primes OCPs for subsequent osteoclast differentiation. Wild‐type but not CX3CR1‐deficient OCPs grown on immobilized FKN showed enhanced osteoclast formation following receptor activator of NF‐κB ligand (RANKL) stimulation, with increased expression of osteoclast differentiation markers. Interestingly, the growth of OCPs on immobilized FKN increased the expression of Cx3cr1 and Tnfrsf11a (Rank) transcripts, but following RANKL stimulation, OCPs rapidly downregulated Cx3cr1 expression. Consistently, anti‐FKN monoclonal antibody (mAb) treatment attenuated RANKL‐induced osteoclast formation on immobilized FKN before, but not during, RANKL stimulation. CX3CR1 and RANK proteins were highly expressed on bone marrow‐derived CD11bhigh CD115+ OCPs. Growth on immobilized FKN prior to RANKL stimulation also increased CD11bhigh CD115+ OCP number and their survival and differentiation potential. In a RANKL‐based mouse model of bone loss, anti‐FKN mAb pretreatment significantly inhibited RANKL‐dependent bone loss. Thus, blocking the FKN‐CX3CR1 axis could represent a therapeutic option in noninflammatory bone loss diseases. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.