bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on Assessing Driving Ability of Older Driver Based on Cognitive Test: A Case Study of Beijing, China
Authors: Gong Jianguo , Guo Xiucheng , Qi Cong , Pan Lingfeng , Liu Xiaochen 
Published: June 2022

Abstract
Research on cognitive tests for the older drivers will contribute to accurately identify those unsafe drivers and decrease the risk of older drivers pose to themselves and other roadway users. This study aims to design and evaluate a comprehensive cognitive test including memory, reaction and judgment ability test. 72 drivers in Beijing, China in 2020 were recruited to participate in this cognitive tests to obtain detailed test information of the recorded response time and accuracy. One-way ANOVA test was proposed to examine the significance among different age and crash records groups. It can be indicated that comprehensive cognitive test was proved effective in judging the impaired older driver, where 96.7% of the safe young group and 100% of the safe older group passed the test, and 89.5% of the caution older group failed the test. The study clarified the efficiency and accuracy of each question as well as the whole test. It also confirmed the driving ability decreased with increase of age. According to the obtained comprehensive cognitive test, it provided a scientific method basis for standardizing the management of the older drivers with a license, so as to guide the older drivers to understand traffic elements and rules.
Effect of human adenovirus 36 on the developing chicken embryo in the context of infectious obesity
Authors: Pogorzelska Aleksandra , Kuropka Piotr , Chełmecka Elżbieta , Kubicka Agata , Janicka Paulina , Stygar Dominika , Bażanów Barbara 
Published: June 2022

Abstract
The obesity epidemic is one of the most serious health problems of the 21st century. The research conducted in this area has allowed for the identification of several factors causing obesity, including infectious agents. Adenovirus 36 (HAdV-D36) is the only known infectious agent to promote obesity in both humans and animals. The mechanisms by which this virus induces obesity in adults are known. However, to date, the effects of this virus on the developing embryo have not been described. In our study, we were able to confirm weight gain in infected HAdV-D36 chicken embryos (ECEs). In contrast to the course of infection in adults, infection in embryos does not result in an increase in body fat, but manifests itself as accelerated organogenesis. Histopathological analyses conducted by our team suggest the existence of another, yet unknown mechanism of the virus's influence on ECE.
Longitudinal clinical and biomarker characteristics of non-manifesting LRRK2 G2019S carriers: The PPMI cohort
Authors: Simuni Tanya , Merchant Kalpana , Brumm Michael , Cho Hyunkeun , Caspell-Garcia Chelsea , Coffey Chris , Chahine Lana , Alcalay Roy , Nudelman Kelly , Foroud Tatiana , Mollenhauer Brit , Siderowf Andrew , Tanner Caroline , Iwaki Hirotaka , Sherer Todd , Marek Ken 
Published: June 2022

Abstract
Background: We examined 2-year longitudinal change in clinical features and biomarkers in LRRK2 non-manifesting carriers (NMCs) versus healthy controls (HCs) enrolled in the Parkinson’s Progression Markers Initiative (PPMI). Methods: We analyzed 2-year longitudinal data from 176 LRRK2 G2019S NMCs and 185 HCs. All participants were assessed annually with comprehensive motor and non-motor scales, dopamine transporter (DAT) imaging and biofluid biomarkers. The latter included cerebrospinal fluid (CSF) Abeta, total tau and phospho-tau; serum urate and neurofilament light chain (NfL); and urine bis(monoacylglycerol) phosphate (BMP). Findings: At baseline, LRRK2 G2019S NMCs had a mean (SD) age of 62 (7.7) years and were 56% female. 13% had DAT deficit (defined as <65% of age/sex-expected lowest putamen SBR) and 11% had hyposmia (defined as ≤15th percentile for age and sex). Although NMCs scored significantly worse on numerous clinical scales at baseline than HCs, there was no longitudinal change in any clinical measures over 2 years or in DAT binding. There were no longitudinal differences in CSF and serum biomarkers between NMCs and HCs. Urinary BMP was significantly elevated in NMCs at all time points but did not change longitudinally. Neither baseline biofluid biomarkers nor DAT correlated with 2-year change in clinical outcomes. Only 5 of 176 LRRK2 NMCs developed PD during the follow up. Interpretation: We observed no significant longitudinal change in clinical or biomarker measures in LRRK2 G2019S NMCs in this large, well-characterized cohort. Longer follow up and further selection of NMCs at highest risk for conversion is required for future prevention clinical trials.
Bacterial assembly as part of immune system in Paulownia responses to witches’ broom caused by phytoplasmas
Authors: Li Xuanzhen , Huang Jing , Zhai Xiaoqiao , Zhao Zhenli , Pan Yanshuo , Fan Guoqiang 
Published: June 2022

Abstract
Purpose
Paulownia witches’ broom (WB) usually affects part of the infected tree, rarely spreading throughout the tree. WB was infected by phytoplasmas, but the associated microbiome assembly remains unknown. This study aims to clarify the possible mediating mechanism of WB outbreaks in Paulownia.
Methods
We collected leaves, branches, roots and rhizosphere soil of Paulownia trees with or without WB, and analyzed the bacterial community on the basis of 16S rRNA high-throughput sequencing.
Results
All leaf and branch samples contained phytoplasmas, but high phytoplasma levels were required for WB. Low bacterial community diversity and richness, a lack of beneficial bacteria, and an unstable bacterial network might be conducive to a WB outbreak. Phytoplasmas were more abundant in the roots of diseased trees than in the roots of healthy trees. Moreover, phytoplasma infections altered the bacterial community composition, but did not induce morphological changes. Phytoplasma did not present in the rhizosphere soil of diseased trees, but their bacterial community composition changed comparing with the corresponding samples of healthy trees. Asymptomatic leaves and branches of diseased trees recruited beneficial bacteria and increased the bacterial network stability, which might contribute to the restriction of phytoplasma infections and WB development further. The abundance of several beneficial bacteria in the endosphere was significantly negative correlation with that of phytoplasmas, implying their inhibitory effects on phytoplasmas.
Conclusion
Our findings have clarified the possible mechanism mediating WB outbreaks in Paulownia trees. Furthermore, we demonstrated that Paulownia trees inhibit phytoplasma infections by modulating the endosphere and rhizosphere bacterial communities.
Process‑based evaluation indicators of grape drought and risk characteristics in Bohai Rim Region (BRR), China
Authors: Huo Zhiguo , Mao Hongdan , Yang Jianying , Wang Peijuan , Wu Dingrong , Ma Yuping 
Published: June 2022

Abstract
Drought stress is one of the major environmental limiting factors for grape growth in the Bohai Rim Region (BRR) in China. Investigation of process-based meteorological evaluation indicators is of particular interest to precisely understand the spatiotemporal characteristics of grape drought processes, providing substantial support for grape drought monitoring, prevention, and mitigation. In this study, a daily index grape drought index (GDI) was constructed based on the rolling calculation of water profit and deficit. Characteristics of GDI in historical drought disaster samples were analyzed, and drought evaluation level indicators were constructed, facilitating the assessment of process-based grape drought risk. The results showed that duration days with GDI<-0.4 identified can be used as drought level indicators, and the threshold of grape drought in germination-new shoot (P1), flowering-fruit setting (P2), fruit expansion (P3), and coloring-mature (P4) were 14, 13, 12, and 13 days with GDI<-0.4, respectively. Identification results calculated by grape drought indicators were basically consistent with historic occurrence levels of grape drought, with 76.9% of historical records completely consistent with the grape level assessment, and 15.4% within one level. The process-based grape drought risk gradually decreased with the advancement of grape growth process, with grape drought risk in the order of P1 > P2 > P3 > P4. Beijing, Tianjin, Hebei and west of Liaoning were detected with the higher drought risk.
Information needs of informal caregivers in caring and rehabilitation for dioxin victims in Vietnam
Authors: Ho Hien Thi , Bui Chi Linh , Santin Olinda , Nguyen Huong Thi , Nguyen Hien Luong Thi , Do Hung Chi , Tran Nghi Ngoc , Tran Tuyet Hanh Thi , Nguyen Anh Mai , Carter Gillian , Bui Ha Thi Thu , Hoang Minh Van 
Published: June 2022

Abstract
Background: Health impacts among Agent Orange/dioxin (dioxin) victims are significant with many individuals requiring daily assistance, informal care, and rehabilitation support. This study aimed to identify the information needs of informal caregivers of dioxin victims in Vietnam. Methods: A cross-sectional study was conducted in Quynh Phu district, Thai Binh province – an area with a large number of dioxin victims, from June 2019 to March 2020. Quantitative data were collected from 124 caregivers of victims via structured interviews. Qualitative data were collected using semi-structured interview guides with in-depth interviews (IDI) (n=36) and two focus group discussions (FGD) (n=12). Results: The results showed that all caregivers of dioxin victims were family members, predominantly older (71.8%), 61.5 years old on average, living on low incomes (97.9%), and were farmers (80.7%). Almost all participants (96.8%) reported having information needs, particularly concerning dioxin’s harms, nutrition, dioxin-related policies and rehabilitation, and psychological support for patients. Caregivers reported that they would like to receive information via health staff counseling (85.0%), television (75.0%), and community loudspeaker (65.8%). Notably, a majority of caregivers reported the need for information regarding psychological support (70.0%). These findings are consistent with qualitative data, which identify an urgent need to provide information, especially through health staff and digital resources. Conclusions: Many families with dioxin victims lived with little support and information, highlighting their high demand for information about care and rehabilitation. Thus, the healthcare system should promote information support, policy, and psychological support for caregivers and victims. An online support system for caregivers and victims is also recommended.
A Case of Intravenous Leiomyoma of Uterus Extending to Pulmonary Artery
Authors: Huang YuanQin , Rao Dan , Zhang Zheng Lu , Gan Quan 
Published: June 2022

Abstract
Background: Uterine intravenous leiomyomatosis is the leiomyoma tissue invaded into the vein outside the leiomyoma ,Reports of extending to the right pulmonary artery are relatively rare. Case presentation: We describe a 31 year-old woman with the benign leiomyoma entended to the right ventricular lumen,, causing mechanical obstruction and corresponding clinical symptoms. The tumor located in the pulmonary artery can cause pulmonary artery obstruction, once diagnosed, surgical treatment should be performed as soon as possible.Conclusions: In this case ,uterine leiomyoma extends to the right pulmonary is clinically more rare.
Psychometric Testing of the Chinese version of the Nursing Home Adjustment Scale: Classical theory Test and Item Response Theory
Authors: Guo Yicong , Liu Siyue , Zhou Feixiang , Hu Zhao , Li Shaojie , Xu Huilan 
Published: June 2022

Abstract
Background
China has the largest number of older people in the world, thus implying a considerable potential demand for relocation to long-term care facilities. It is critical to developing effective measurement instruments for assessing the adaptation to nursing homes among older adults in China. The nursing home adjustment scale (NHAS) was designed for assessing nursing home adjustment and has been translated into Chinese recently. However, the evidence for the psychometric properties of the Chinese version is insufficient, with the lack of validation in less developed regions of China and item response theory (IRT)-based analysis.
Methods
The English version of the NHAS was translated into Chinese according to the standard procedures. A total of 761 older adults who lived in 21 nursing homes in Hunan Province, China, were investigated from July 2021 to December 2021. The psychometric characteristics of the Chinese version of the NHAS (C-NHAS) were examined based on the classical test theory (CTT) and item response theory (IRT).
Results
The overall Cronbach’s α coefficient of the C-NHAS was 0.913. The 4-factor solution (CFI = 0.958, TLI = 0.951, and RMSEA = 0.049) was regarded as applicable to the C-NHAS: depressed mood, emotional stress, relationship development, and sense of belonging. Rasch analysis showed the C-NHAS was multidimensional. The information-weighted fit statistic (infit) and outlier-sensitive fit statistic (outfit) mean square (MNSQ) values of the subscales of C-NHAS were 0.64–1.42 and 0.64–1.46, indicating all items showed an acceptable model fit. No items displayed substantial differential item functioning (DIF) across gender. In the Wright map, the difficulty of items matched most individuals’ ability, while a small number of individuals with the low latent trait had no items for matching.
Conclusions
In summary, the C-NHAS is a reliable and valid instrument that can assist in measuring nursing home adjustment among Chinese seniors. Rasch analysis results support the multidimensional and DIF-free across gender of the C-NHAS. The difficulty of the NHAS may restrict the measurement of adjustment, thus further development and testing of a brief version assessing adjustment in the nursing home population is worth considering.
Fast-growing exotic tree species as fuelwood alternative for refugees and host communities in Northern Uganda
Authors: Anywar Decimon , Shaban Kato Stonewall , Labeja Richard Louis , Loki Robert Okongo , Okello Collins 
Published: June 2022

Abstract
The high demand for firewood and charcoal has exerted high pressure on the indigenous trees in Uganda. Communities believe that the indigenous trees are better fuel sources despite limited evidence to support this claim. This study was carried out in 2021 to evaluate the fuelwood properties of selected indigenous tree species in comparison to three exotic tree species that have been promoted for use by refugees and host communities in Lamwo District of Northern Uganda. Wood samples were collected from three different locations from the study area in Lamwo District and transported to Gulu University for laboratory analysis of their physical and chemical properties. Data were analysed using one way analysis of variance (ANOVA) at 5% level of confidence and the means were separated using Tukey HSD test. Results showed that moisture content, fixed carbon, volatile matter, and Fuel Value Index (FVI) did not significantly vary among the indigenous and exotic tree species. Fuel value index was observed to be negatively correlated with moisture content of the wood, implying both species’ categories retard in fuel quality when their moisture contents increases. Overall, the results show that there were no significant differences in the FVI of all the indegeneous and exotic tree species investigated in this study. It is recommended that fast-growing exotic species such as Eucalyptus grandis , Caliandra calothyrsus , and Senna siamea that are grown in the region be promoted to ensure regeneration and reduction of pressure on the use of natural forest.
Effective use of slag as a product of the CFBC technology to purify water environment of copper ions
Authors: Kalak Tomasz 
Published: June 2022

Abstract
The world of today is faced with many problems related to global pollution of the aquatic environment, including groundwater, rivers, lakes, bays, seas and oceans with substances harmful to ecosystems and living organisms. Water plays a major role in maintaining life, hence its quality is crucial in the entire food chain. One of the greatest threats to the aquatic environment is industrial wastewater containing harmful heavy metals and other toxic substances. For this reason, it is necessary to search for new, cheap and clean technologies for removing metals from wastewater. In this study, slag was used, which is a waste obtained in the modern and clean circulating fluidized bed combustion technology (CFBC), considered to be the most effective method of incineration of municipal sewage sludge. The physical and chemical properties of the slag material were characterized, such as grain composition, bulk density, SEM-EDS analysis, thermogravimetric measurements, BET adsorption and analysis of SEM and TEM images. Then, adsorption properties of slag in relation to Cu(II) ions in aqueous solutions were investigated. The research results showed high adsorption process efficiency and adsorption capacity. On the basis of the obtained results, it was found that the slag waste generated in the CFBC technology may be a cheap and highly effective adsorbent to be used in adsorption processes to remove heavy metals from wastewater.
Sex-based differences in the lipidomic profile of circulating extracellular vesicles isolated from adolescents exposed to ethanol intoxication
Authors: Perpiñá-Clérigues Carla , Català-Senent José F. , Mellado Susana , Ibáñez Francesc , Costa Pilar , Marcos Miguel , Guerri Consuelo , García-García Francisco , Pascual Maria 
Published: June 2022

Abstract
Background
Lipids represent essential components of extracellular vesicles (EVs), playing structural and regulatory functions during EV biogenesis, release, targeting, and cell uptake. Importantly, lipidic dysregulation has been linked to several disorders, including metabolic syndrome, inflammation, and neurological dysfunction. Our recent results demonstrated the involvement of plasma EV microRNAs as possible amplifiers and biomarkers of neuroinflammation and brain damage induced by ethanol intoxication during adolescence. Considering the possible role of EV lipids as regulatory molecules and biomarkers, we evaluated how acute ethanol intoxication differently affected the lipid composition of plasma EVs in male and female adolescents and explored the participation of the immune response.
Methods
Plasma EVs from humans, WT mice, and TLR4-KO mice were extracted. After the EVs lipids extraction and data acquisition by mass spectrometry, preprocessing and exploratory analyses were conducted. Experimental groups (ethanol and control groups of females and males) were compared from differential abundance analysis. Annotation of the lipids in their corresponding classes and a lipid set enrichment analysis were carried out to evaluate the biological function.
Results
We demonstrate for the first time that acute ethanol intoxication induces a higher enrichment of specific EV lipid species in human female adolescents (e.g., PA, LPC, unsaturated FA and FAHFA) than in males (e.g., PI). These lipid species participate in the formation, release, and uptake of EVs and the activation of the immune response. All data and results generated have been made openly available on a web-based platform (http://bioinfo.cipf.es/sal).
Conclusions
Our results suggest that female adolescents who binge drink alcohol also display increased levels of EV biogenesis and neuroinflammatory spread than males. Our findings also support the potential use of EV-enriched lipids as biomarkers of ethanol-induced neuroinflammation during adolescence.
Impact of land use on the soil microbiota and soil abiotic traits in tropical and subtropical Brazilian ecoregions
Authors: de Souza Tancredo Augusto Feitosa , Silva Lucas Jónatan Rodrigues da , Laurindo Lídia Klestadt , Nascimento Gislaine dos Santos , Campos Milton César Costa 
Published: June 2022

Abstract
Land uses influence soil microbiota and soil abiotic traits in the Brazilian Tropical and Subtropical ecoregions. Our aim was to test if different land uses may influence the soil microbiota and the soil chemical properties in agroforestry systems (AF), unassisted forest restoration (UFR), and natural ecosystem (Ne) located at Tropical and Subtropical ecosystems from North-eastern and Southern, Brazil, respectively. We have used the joint analysis of a series of experiments in a randomized block design. In the three land uses, we collected soil samples to determine arbuscular mycorrhizal fungi community, soil nematode, soil pH, available P, and soil organic carbon (SOC) from January 2018 to January 2022 using a semestral schedule. Soil microbiota richness in the AF and Ne was similar in the tropical ecosystem. The PCA analysis showed SOC, soil pH, Fungivores, Bacterivores, Racocetra coralloidea, Dentiscutata scutata, and D. erythrophus as the main factors contributing to the variance of the samples. Differences were associated with (1) the land uses by promoting different habitat, as we found difference on soil microbiota abundance, and diversity as affected by habitat simplification, and (2) changes in soil pH, SOC, and available P. These results contribute to a deeper view of the soil microbiota community composition in different land uses from two Brazilian ecosystems. Our work increases the understanding of the soil microbiota com-munity structure in the rhizosphere of AF and Ne in tropical and subtropical ecosystems.