bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neorhizobium xiangyangense sp. nov., Isolated From a Highland Barley Cultivation Soil in Qamdo, Tibet
Authors: Pan Hu , Zhou Zi-Qiong , He Guo-Wei , Zhou Jie , Jin Kai , Liu Hu-Hu , Wang Chong , Yang Hui , Lu Xiang-Yang , Yu Yao-Bin , Tian Yun 
Published: March 2022

Abstract
A novel gram-negative, aerobic, rod-shaped and non-nitrogen fixing bacterium named strain T786T was isolated from a highland barley cultivation soil in Qamdo, Tibet Autonomous Region, PR China. Strain T786T grew at 5–30℃ and pH 6.0–10.0 (optimum, 20–25℃ and pH 7.0–8.0) with 0–4% (w/v) NaCl (optimum, 0%). The 16S rRNA gene sequences of strain T786T showed the highest similarity to Neorhizobium vignae CCBAU 05176T (98.70%), followed by Neorhizobium alkalisoli CCBAU 01393T (98.47%), Neorhizobium tomejilense T17_20T (98.43%), Neorhizobium huautlense S02T (98.36%), and Neorhizobium galegae ATCC 43677T (98.00%). Phylogenetic analysis based on 16S rRNA genes indicated that strain T786T was a new member of the genus Neorhizobium. The digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain T786T and related strains were 20.20–20.60% and 76.64–80.01%, respectively. The genomic DNA G + C content of strain T786T was 60.2%. The major cellular fatty acids were Summed feature 8 (C18:1 ω7c or C18:1 ω6c, 51.53%), C16:0 (21.74%) and Summed feature 3 (C16:1 ω7c or C16:1 ω6c, 10.21%). The polar lipids of strain T786T were consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl methyl ethanolamine, unidentified phospholipid and unidentified lipids (1–4). The isoprenoid quinones of strain T786T was consisted of Q-10. The DAP and sugar components of cell wall of strain T786T were meso-DAP and ribose, glucose, respectively. Based on phenotypic, phylogenetic, and genotypic datas, strain T786T is considered to represent a novel species of the genus Neorhizobium, for which the name Neorhizobium xiangyangense sp. nov. is proposed. The type strain is T786T (= JCM 35100T = CICC 25102T).
Heat transfer and hybrid nanofluid flow over a porous stretching/shrinking sheet with Brinkmann model and multiple slips
Authors: Botmart Thongchai , Mahabaleshwar U. S. , Vishalakshi A. B. 
Published: March 2022

Abstract
The current study explains the hybrid nanofluid flow and transfer of heat with radiation and mass transpiration. The velocity slips and thermal jump condition is also taken in governing equation respectively. The solid volume fractions of Al2O3-Cu/H2O hybrid nanofluid are considered tin the entire calculation. The governing PDEs are altered into ODEs with the help of similarity variable. Hybrid nanofluid is used to increase the efficiency of heat transfer and in the field of industrial applications. The role of Brinkmann model is considered in the momentum equations. Momentum equation is solved analytically to get the 4th root of the equation and also get the solution domain. This domain is used in the energy equation and then it is solved analytically and expressed in terms of Kummer’s function. The existence and non-existence solutions for both stretching and shrinking sheet is investigated. Shrinking sheet case exhibit various behavior compared to stretching sheet case and produces physically meaningful dual solutions. The results of velocity and temperature profiles can be analyzed with the help of different controlling parameters namely mass transpiration, thermal radiation, solid volume fractions, Brinkmann number, and porous medium parameter. Stability analysis can be performed to extend the results of the present study.
A study on dose and time-dependent effect of fluoride in rats
Authors: Validandi Vakdevi , Khandare Arjun , Dheeravath Srinivas , Venkata SurekaMullapudi , Kurella Srinivasu , Sinha Sukesh Narayan 
Published: March 2022

Abstract
The purpose of this study was to see the effect of fluoride (F) in Wistar NIN rats in a dose and time-dependent manner. Male rats (n=36) were taken and were randomly divided into six groups (Group I: control, group II: 5 mg/L F, Group III: 10 mg/L F, Group IV: 15 mg/L F, Group V: 50 mg/L F and Group VI: 100 mg/L F). Animals were examined at 3-time points of 30, 60 and 90 days for F toxicity changes. The results indicated that the F induced dose and time-dependent diet intake, body weight gain, food efficiency ratio, dental fluorosis, urinary, serum, bone and teeth F levels and histopathological changes in liver and kidney tissue. The significant increase in exposure of F through water in groups V and VI as compared to other groups has shown alterations in their food efficiency ratio, body weight gain, and the significant increase in the urinary and serum F levels. Histopathology of the kidney showed few dilated tubules in the kidney of group VI compared to all other groups. Whereas histopathology of the liver showed microvacuolation in groups V and VI compared to groups I, II and III. However, there were no significant differences between the organ weight ratios of different organs among all the groups. In conclusion, the dose-dependent effect of F starts at 100, 50 and 15mg/L from first, second and third months respectively, however, there were no significant differences in 5 and 10 mg/L F groups after 3 months.
Distribution, Source finding, ecological hazard assessment and water-sediment exchange rate of polychlorinated biphenyl compounds (PCBs) in South Pars Industrial Zone, Iran
Authors: Ghadrshenas Alireza , Tabatabaie Tayebeh , Amiri Fazel , Pazira Abdul Rahim 
Published: March 2022

Abstract
South Pars Industrial Zone is located near an Assaluyeh city on the coast of the Persian Gulf and is known as the energy capital of Iran. In this study, environmental and health effects due to PCB components had an assessment. In this study, 10 air stations, 10 seawater, and sediments stations were systematically selected and sampled in two seasons. Air, seawater and sediment pollution made by polychlorinated biphenyls (PCBs) were evaluated. Seawater-sediment exchange conditions using the fugacity coefficient reviewed. PCB levels in marine sediments, seawater, and air-based on the analysis of the obtained data were 107.33-172.92 ng / g, ND-135.68 μg / l, and ND-4.4 ng / m3, respectively. The highest concentration was observed in the vicinity of refineries, petrochemicals, and petroleum export facilities. These values had increased significantly compared to values of studies, conducted in similar areas. The sources were electrical wastes, storage sites, power generation units, and wastewater treatment. The ecological risk of seawater was assessed to be high, while sediment risks were reported with a low to moderate risk range. The heavier compounds with more chlorine were moved from sediments to seawater and, showed that seabed acted as a secondary source of sea pollution. Lighter compounds with less chlorine moved toward the sediments, from the seawater, and the seabed acted as a sink for these compounds. It is suggested for the area to be continuously monitored while engineering and management measures should be adopted to improve the situation and also prevent the spread of pollution.
Oncogenic protein condensates suppress growth factor perception and modulate drug tolerance
Authors: Bugaj Lukasz , Gonzalez-Martinez David , Roth Lee , Mumford Thomas , Guan Juan , Huang Bo , Tulpule Asmin , Bivona Trever G. 
Published: March 2022

Abstract

Drug resistance remains a central challenge towards durable cancer therapy, including for cancers driven by the EML4-ALK oncogene. EML4-ALK and related fusion oncogenes form cytoplasmic protein condensates that transmit oncogenic signals through the Ras/Erk pathway. However, whether such condensates play a role in drug response is unclear. Here, we used optogenetics to find that condensates suppress signaling through endogenous RTKs including EGFR. Notably, ALK inhibition hypersensitized RTK signals, which are known to drive resistance. Suppression of RTKs occurred because condensates sequestered downstream adapter proteins that are required for RTK signal transmission. Strikingly, EGFR hypersensitization resulted in rapid and pulsatile Erk signal reactivation, which originated from neighboring apoptotic cells. Paracrine signals promoted survival during ALK inhibition, and blockade of paracrine signals suppressed drug tolerance. Our results uncover a regulatory role for RTK fusion condensates in cancer drug response and demonstrate the potential of optogenetics for uncovering functional biomarkers of cancer cells.
5-year adherence to adjuvant endocrine treatment in Dutch women with early stage breast cancer: a population-based database study (2006-2016).
Authors: Biggelaar Yannick van den , Kuiper Josine , Sangen Maurice J.C. vander , Luiten Ernest , Siesling Sabine , Herk-Sukel Myrthe van , Voogd Adri , Mesters Ilse 
Published: March 2022

Abstract
PurposeHormonal receptor (HR) positive breast tumors are common. Adjuvant hormonal therapy (AHT) with Tamoxifen or Aromatase Inhibitors (AIs) is beneficial depending on the stage of the tumor. Despite the fact that AHT has been shown to improve survival and recurrence, Dutch adherence rates, which were mostly dependent on Tamoxifen prescriptions until 2006, plummeted from 80% after one year to 50% after five years. Nonadherence with AHT reduces its effectiveness. This research presents more recent adherence statistics (from 2006 to 2016) as well as factors that influence AHT adherence. In addition to Tamoxifen data, AIs are now included in prescriptions.MethodsThe Netherlands Cancer Registry (NCR) was used to find women with early-stage breast cancer who started AHT within a year of surgery and were linked to the PHARMO Database Network. The Kaplan-Meier approach was used to measure AHT adherence five years after treatment was started, with a 60-day gap between refills as our primary outcome. Furthermore, the Medication Possession Rate (MPR) was determined using a cutoff of 50%. Subgroup analysis was performed on influential factors of adherence.Results The 5-year adherence rate was 46.6% when gaps of 60 days between refills were allowed. Based on MPR 80 %, the five-year adherence rate was 53.3%. Age, ≥ 70 years (HR (95% CI) 1.20 (1.10 – 1.30), 50-69 years (HR (95 % CI) 1.09 (1.02 – 1.17) vs < 49 years), and year of diagnosis, 2011-2016 (HR (95 percent CI) 2.39 (2.24-2.56) vs 2006-2010) were also linked with adherence continuance.ConclusionDutch 5-year AHT adherence has remained poor.
Evaluation of the convection-permitting regional climate model CNRM-AROME41t1 over northwestern Europe
Authors: Lucas-Picher Philippe , Brisson Erwan , Caillaud Cécile , Alias Antoinette , Nabat Pierre , Lemonsu Aude , Poncet Nils , Hernandez Virginia Edith Cortes , Michau Yohanna , Doury Antoine , Monteiro Diego , Somot Samuel 
Published: March 2022

Abstract
Since a decade, convection-permitting regional climate models (CPRCM) have emerged showing promising results, especially in improving the simulation of precipitation extremes. In this article, the CPRCM CNRM-AROME developed at the Centre National de Recherches Météorologiques (CNRM) since a few years is described and evaluated using a 2.5-km long 19-year hindcast simulation over a large northwestern European domain using different observations through an added-value analysis in which a comparison with its driving 12-km RCM CNRM-ALADIN is performed. The evaluation is challenging due to the lack of high-quality observations at both high temporal and spatial resolutions. Thus, a high spatio-temporal observed gridded precipitation dataset was built from the collection of seven national datasets that helped the identification of added value from CNRM-AROME. The evaluation is based on a series of standard climatic features that include long-term means and mean annual cycles of precipitation and near-surface temperature where CNRM-AROME shows little improvements compared to CNRM-ALADIN. Additional indicators such as the summer diurnal cycle and indices of extreme precipitation show, on the contrary, a more realistic behaviour of the CNRM-AROME model. Moreover, the analysis of snow cover shows a clear added-value in the CNRM-AROME simulation, principally due to the improved description of the orography with the CPRCM high resolution. Additional analyses include the evaluation of incoming surface longwave and shortwave radiation, and cloud cover using satellite estimates. Overall, despite some systematic biases, the evaluation indicates that CNRM-AROME is a suitable CPRCM that is superior in many aspects to the RCM CNRM-ALADIN.
Analysis of spot urine biomarkers and association with body weight in Japanese elementary school children
Authors: Takemoto Nozomi , Millman Jasmine , Yamakawa Fusae , Uema Tsugumi , Okamoto Shiki , Mori Mari , Mori Hideki , Nakamura Koshi , Yamori Yukio , Masuzaki Hiroaki 
Published: March 2022

Abstract
Purpose
Childhood obesity is rapidly increasing world-wide and is largely the consequence of adoption of unhealthy diets excessive in calories and salt (NaCl) as well as devoid in pivotal micronutrients such as potassium (K) and magnesium (Mg). Education-based programs aiming to encourage healthy food knowledge and behaviors are crucial at a young age, and for this purpose, convenient ways to assess daily dietary intake are warranted. We therefore attempted to evaluate the dietary intake of Okinawan school children in Japan by analyzing a series of biomarkers in morning spot urine samples and explore whether these biomarkers correlate with body weight and a series of metabolic parameters.
Methods
We enrolled 98 third-grade elementary school children in Okinawa, Japan. Morning spot urine samples were collected and analysed using high performance liquid chromatography (HPLC) to assess dietary intake.
Results
We found that estimated daily NaCl intake was higher in obese/overweight children as compared to healthy-weight children (p = 0.0001). There was also a significant positive correlation between body mass index (BMI) and NaCl intake (Spearman) (ρ = 0.45, p < 0.0001) and a negative correlation between BMI and Mg/Cr (ρ=-0.27, p = 0.01). Furthermore, Na/K ratio was higher in samples collected on Monday (weekend) as compared to samples collected on Thursday or Friday (weekday) (p < 0.0001).
Conclusion
Via the use of morning spot urine analyses, our results show that NaCl intake was associated with obesity, and Mg excretion negatively correlated with BMI in Japanese school children, highlighting the potential role of these micronutrients in maintaining a healthy body weight.
A novel immune-related eRNAs prognostic signature to predict the immunotherapy response for thyroid carcinoma
Authors: Wu Pu , Shi Jinyuan , Wang Zhiyuan , Sun Wei , Zhang Hao 
Published: March 2022

Abstract
Background
The functional alterations of eRNAs have been reported to be correlated with tumorigenesis. However, the roles of eRNAs in thyroid cancer (THCA) remain still unclear. This study aimed to construct an immune-related eRNA prognostic signature that could effectively predict the survival and prognosis for THCA.
Methods
The Weighted Gene Co-Expression Network Analysis (WGCNA) was performed to identify THCA-specific immune-related hub genes and immune-related eRNAs were obtained using Pearson correlation analysis. Univariate and least absolute shrinkage and selection operator (LASSO) Cox regression were conducted to construct an immune-related eRNA prognostic signature in training cohort, and the predictive capability was verified in test cohort and entire cohort. Kaplan-Meier analysis, principal component analysis (PCA), receiver operating characteristic (ROC) curves, and nomogram were used to validate the risk signature. Furthermore, CIBERSORT, ESTIMATE and ssGSEA were analyzed to explore the tumor immune microenvironment (TIME) of the risk signature, and the response of potential immunotherapeutic were also discussed.
Results
A total of 125 immune-related eRNAs were obtained and 16 immune-related eRNAs were significantly correlated with overall survival (OS). A 9-immune-related eRNA prognostic signature was constructed, and the risk score was identified as an independent predictor. High-risk groups were associated with a poorer OS. Immune microenvironment analysis indicated that low risk score was correlated with higher immuneScore, high immune cell infiltration, and the better response of immunotherapy. Additionally, we also detected 9 immune-related eRNA expression levels in sixty-two matched tumorous and non-tumorous tissues using qRT-PCR analysis.
Conclusion
Our immune-related eRNA risk signature that was an independent prognostic factor was strongly correlated with the immune microenvironment and may be promising for the clinical prediction of prognosis and immunotherapeutic responses in THCA patients.
Activity of Tixagevimab/Cilgavimab against the Omicron variant of SARS-CoV-2 in a hamster model
Authors: DRIOUICH Jean-Sélim , Lingas Guillaume , Luciani Léa , Cochin Maxime , Villarroel Paola Mariela Saba , Moureau Gregory , Petit Paul Rémi , Touret Franck , Guedj Jeremie , de Lamballerie Xavier , Nougairède Antoine 
Published: March 2022

Abstract
The efficacy of pre-exposure prophylaxis by the Tixagevimab/Cilgavimab cocktail (AZD7442) was evaluated in hamsters against a clinical BA.1 strain of SARS-CoV-2 variant Omicron. AZD7442 retains inhibitory activity against Omicron despite a substantial loss of efficacy. We estimate that Omicron virus requires about 20-times more antibodies in plasma than the ancestral B.1 strain (G614) virus to achieve a similar drug efficacy in reducing lung infectious titers.
The perinatal and childhood outcomes of children born to Indigenous women with mental health problems: A scoping review
Authors: Adane Akilew A , Shepherd Carrington CJ , Reibel Tracy , Ayano Getnet , Marriott Rhonda 
Published: March 2022

Abstract
Purpose
Maternal mental health problems are common during the perinatal period and have been associated with several negative outcomes in children. However, few studies have examined these associations among Indigenous people, and the findings across these studies have been inconsistent. This scoping review comprehensively examined the perinatal and childhood (≤ 12 years) health and development outcomes of the children of Indigenous women with perinatal mental health problems.
Methods
Following the Preferred Reporting Items for Systematic reviews and Meta-Analyses guidelines, Medline, CINAHL, PsycINFO, Joanna Briggs Institute, Wed of Science, Scopus, and other databases were searched electronically for studies examining the associations between any perinatal maternal mental health problems and perinatal and child outcomes among the Indigenous population of Australia, Canada, New Zealand, and the USA. Two authors reviewed studies for inclusion.
Results
Of 2,038 records identified, 11 were eligible. One of four studies that evaluated the associations between maternal depression, stress, and anxiety problems, and perinatal and childhood behavioural outcomes found a negative association. Six of seven studies (five used similar cohort data) that examined the associations of maternal substance use disorder and several perinatal and childhood outcomes found at least one negative association.
Conclusions
Maternal substance use disorder appears to be associated with adverse perinatal and childhood outcomes among the Indigenous population. However, there is preliminary evidence for the other common maternal mental health problems. Further research is therefore critically required to draw definitive conclusions regarding the impact of maternal mental health problems on the perinatal and childhood outcomes.
War-related Burns in Syria in the Years 2013-2017 A Cross Sectional Study in Damascus Hospital
Authors: Haffar Massa , Aissami Maen Al 
Published: March 2022

Abstract
Intorduction:Thermal injuries have always been a source of morbidity and mortality in times of war. Historically, they constitute 5% to 20% of all injuries and approximately 4% mortality.This study will review the information acquired from patient’s files on war-related thermal injuries in Damascus Hospital from 2013 to 2017. We will describe the epidemiology of burn injuries, and the assessment, and final treatment of burn patients.Methods:A cross sectional study of war-related burn injuries was conducted during the years 2013-2017 in Damascus Hospital. Chi-squre analysis was used to compare gender and mortality with sociodemographic factors and burn outcome variables. One-way analysis of variance (anova) and t-test were used to compare means of continuous variables (Age, TBSA,ABSI).Results:450 patients were included in this study. 289 (64.2%) were males and 207 (46%) were from the eastern region of Syria. Most injuries were flame injuries 197 patients (43.8%) and petroleum substance burns 93 (20.7%). Most common injury place was the four limbs 288 (28.4%), and head and neck 282 (27.8%). Mortality rates were 163 (36.2%) and the most common cause of death was respiratory failure 80 (49.1%). Gender and mortality were significantly associated with sociodemographic factors and burn outcomes (P-value<0.05).Conclusion:More details have been identified regarding war related burn injuries in Syria. This leads to conduct better health care programs and stress the importance of better triage with war circumstances. Additional studies are required to determine management and to get better insight on the treatment.
Systematic Review and Meta-analysis of Randomized Controlled Trials of Ayurvedic and Herbal dentifrices for Plaque reduction in Gingivitis
Authors: Javed Danish , Dixit Ashish Kumar , Anwar Sana , Rai Anshul 
Published: March 2022

Abstract
Background
Despite the existence of a large number of trials employing ayurvedic and herbal dentifrices to reduce plaque in gingivitis, the findings are inconsistent. Therefore, we assess the overall effect of ayurvedic and herbal dentifrices in gingivitis on dental plaque index (PI), gingival index and bacterial colony counts (CC) of debris.
Methods
We found 554 articles after searching PubMed, CAM-QUEST®, and the Cochrane Central Register of Controlled Trials. 41 randomised clinical trials were considered for meta-analysis.The Standard Mean Difference (SMD) for effect size estimation with 95% confidence intervals (CI) was estimated using RevMan 5.4.1 software.
Results
A total 2,806 patients in 41 RCTs were participated. Ayurvedic dentifrices,Plaque index (PI): SMD= -0.52, 95% CI (-0.94,-0.11); CC: SMD= -1.70, 95% CI (-5.06,1.67), (Oil pulling therapy :PI: SMD= -0.38, 95% CI (-1.45,0.68); CC: SMD= -1.04, 95% CI (-2.20,0.11), Herbal Dentifrices :PI: SMD= -0.58, 95% CI (-1.55,0.39), (Triphala :PI: SMD= -0.65, 95% CI (-1.32,0.03) were found as good as control.
Conclusions
In addition to conventional dental care, ayurvedic and herbal dentifrices may help in plaque reduction, gingival inflammation, and bacterial growth. They are safe and may be recommended in community dentistry practices.