bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using axenic and gnotobiotic insects to examine the role of different microbes on the development and reproduction of the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae)
Authors: Gilliland Carissa A. , Patel Vilas , Dombrowski Ashley C. , Mackett Bradley M. , Vogel Kevin J. 
Published: June 2022

AbstractKissing bugs (Hempitera: Reduviidae) are obligately and exclusively blood feeding insects. Vertebrate blood is thought to provide insufficient B vitamins to insects, which rely on obligate symbiotic relationships with bacteria that provision these nutrients. Kissing bugs harbor environmentally acquired bacteria in their gut lumen, without which they are unable to develop to adulthood. Early experiments identified a single bacterial species, Rhodococcus rhodnii, as a symbiont of Rhodnius prolixus, but modern studies of the kissing bug microbiome suggest that R. rhodnii is not always present or abundant in wild-caught individuals. We asked whether R. rhodnii or other bacteria alone could function as symbionts of R. prolixus. Bacteria-free (axenic) insects were produced whose microbiome could be experimentally manipulated to produce insects with known microbiomes (gnotobiotic). We found that gnotobiotic insects harboring R. rhodnii alone developed faster, had higher survival, and laid more eggs than gnotobiotic R. prolixus harboring other bacterial monocultures, including other described symbionts of kissing bugs and several related Rhodococcus species. R. rhodnii grew to high titer in the guts of R. prolixus while other tested species were found at much lower abundance. Rhodococcus species tested had nearly identical B vitamin biosynthesis genes, and dietary supplementation of B vitamins had a relatively minor effect on development and survival of gnotobiotic R. prolixus. Our results indicate that R. prolixus have a higher fitness when harboring R. rhodnii than other bacteria tested, and that symbiont B vitamin synthesis is likely a necessary but not sufficient function of gut bacteria in kissing bugs.
Helicobacter pylori provokes STING immunosurveillance via trans-kingdom conjugation
Authors: Damke Prashant P. , Wood Cecily R. , Shaffer Carrie L. 
Published: June 2022

ABSTRACTRecognition of foreign nucleic acids is an evolutionarily conserved mechanism by which the host detects microbial threats. Whereas some intracellular bacterial pathogens trigger DNA surveillance pathways following phagosomal membrane perturbation, mechanisms by which extracellular bacteria activate cytosolic nucleic acid reconnaissance systems remain unresolved. Here, we demonstrate that Helicobacter pylori exploits cag type IV secretion system (cag T4SS) activity to provoke STING signaling in gastric epithelial cells. We provide direct evidence that chromosomal fragments delivered to the host cell cytoplasm via trans-kingdom conjugation bind and activate the key DNA sensor cGMP-AMP synthase. To enable paracrine-like signal amplification, translocated H. pylori DNA is sorted into exosomes that stimulate DNA-sensing pathways in uninfected bystander cells. We show that DNA cargo is loaded into the cag T4SS apparatus in the absence of host cell contact to establish a ‘ready-to-fire’ nanomachine and provide evidence that cag T4SS-dependent DNA translocation is mechanistically coupled to chromosomal replication and replichore decatenation. Collectively, these studies suggest that H. pylori evolved mechanisms to stimulate nucleic acid surveillance pathways that regulate both pro- and anti-inflammatory programs to facilitate chronic persistence in the gastric niche.
Candidate targets of copy number deletion events across 17 cancer types
Authors: Huang Qingyao , Baudis Michael 
Published: June 2022

AbstractGenome variation is the direct cause of cancer and driver of its clonal evolution. While the impact of many point mutations can be evaluated through their modification of individual genomic elements, even a single copy number aberration (CNA) may encompass hundreds of genes and therefore pose challenges to untangle potentially complex functional effects. However, consistent, recurring and disease-specific patterns in the genome-wide CNA landscape imply that particular CNA may promote cancer-type-specific characteristics. Discerning essential cancer-promoting alterations from the inherent co-dependency in CNA would improve the understanding of mechanisms of CNA and provide new insights into cancer biology and potential therapeutic targets. Here we implement a model using segmental breakpoints to discover non-random gene coverage by copy number deletion (CND). With a diverse set of cancer types from multiple resources, this model identified common and cancer-type-specific oncogenes and tumor suppressor genes as well as cancer-promoting functional pathways. Confirmed by differential expression analysis of data from corresponding cancer types, the results show that for most cancer types, despite dissimilarity of their CND landscapes, similar canonical pathways are affected. In 25 analyses of 17 cancer types, we have identified 19 to 169 significant genes by copy deletion, including RB1, PTEN and CDKN2A as the most significantly deleted genes among all cancer types. We have also shown a shared dependence on core pathways for cancer progression in different cancers as well as cancer type separation by genome-wide significance scores. While this work provides a reference for gene specific significance in many cancers, it chiefly contributes a general framework to derive genomewide significance and molecular insights in CND profiles with a potential for the analysis of rare cancer types as well as non-coding regions.
Sub-operon promoter arrangement of disA facilitates c-di-AMP homeostasis and selective stress responses in M. smegmatis
Authors: Singla Mamta , Pal Aditya Kumar , Chaudhary Vikas , Ghosh Anirban 
Published: June 2022

AbstractBacterial second messenger signaling often plays an important role in cellular physiology. In this study, we have attempted to understand how c-di-AMP synthesis and degradation are transcriptionally regulated in M. smegmatis. We have discovered that, although c-di-AMP synthesis gene disA exists in a multi-gene operon; a sub-operon promoter arrangement plays a key role under various stress conditions, keeping its dual function property intact. Further, we learned that c-di-AMP plays a role in the autoregulation of the disA promoter to limit intracellular c-di-AMP concentration. We also identified an alternate start codon within the disA gene which can lead to the synthesis of truncated DisA protein at times using an independent stress-inducible promoter. All in all, this study was helpful to understand how c-di-AMP synthesis is regulated under normal and stress conditions linked to its physiological relevance in M. smegmatis.
Mutations in rv0678 confer low-level resistance to benzothiazinone DprE1 inhibitors in M. tuberculosis
Authors: Poulton Nicholas C. , Azadian Zachary A. , DeJesus Michael A. , Rock Jeremy M. 
Published: June 2022

ABSTRACTTuberculosis (TB) is the leading cause of death from any bacterial infection, causing 1.5 million deaths worldwide each year. Due to the emergence of drug-resistant strains of Mycobacterium tuberculosis (Mtb) there have been significant efforts aimed at developing novel drugs to treat TB. One promising drug target in Mtb is the arabinogalactan biosynthetic enzyme DprE1, and there have been over a dozen unique chemical scaffolds identified which inhibit the activity of this protein. Among the most promising lead compounds are the benzothiazinones BTZ043 and PBTZ169, both of which are currently in or have completed phase IIa clinical trials. Due to the potential clinical utility of these drugs, we sought to identify potential synergistic interactions and new mechanisms of resistance using a genome-scale CRISPRi chemical-genetic screen with PBTZ169. We found that knockdown of rv0678, the negative regulator of the mmpS5/L5 drug efflux pump, confers resistance to PBTZ169. Mutations in rv0678 are the most common form of resistance to bedaquiline and there is already abundant evidence of these mutations emerging in bedaquiline-treated patients. We confirmed that rv0678 mutations from clinical isolates confer low level cross-resistance to BTZ043 and PBTZ169. While it is yet unclear whether rv0678 mutations would render benzothiazinones ineffective in treating TB, these results highlight the importance of monitoring for clinically-prevalent rv0678 mutations during ongoing BTZ043 and PBTZ169 clinical trials.
How to elevate the quantity and quality of contributors for crowdsourcing tasks: the double-edged role of task reward
Authors: Wang Dianwen , Mou Yupeng , Ding Zhihua , Jiang Xuehui 
Published: June 2022

PurposeCrowdsourcing refers to a new business model in which enterprises or individuals publish tasks or problems, attracting freelancers or contributors to participate in solving tasks, submitting bids and allowing task seekers to choose the final solution. How to attract more quantity and quality of contributors to submit their solutions through a crowdsourcing platform has become a vital question.Design/methodology/approachIn this study, the authors use web crawling to obtain 43,265 effective tasks in EPWK website (www.epwk.com) to probe how to elevate the quantity and quality of contributors via task reward design. This study uses the hierarchical linear model to probe the research questions.FindingsResults show that, with the increase of task reward, the quantity of contributors goes up first and then goes down (inverted U shape), whereas the quality of contributors goes down first and then goes up (U sharp). Moreover, the authors investigate the moderating effects of another task design attribute, task duration. This study finds that task duration weakens the effect of task reward on the quantity of contributors while strengthening the effects of task reward on the quality of contributors.Originality/valueFirst, this study theoretically probes two key aspects of task performance, namely, the quantity and quality of contributors, which expand the scope of task performance evaluation. Second, this study reconciles previous concern about the relationship of task reward and performance, which is different from previous studies that have paid more attention to the single perspective of their relationship. Finally, the authors investigate the moderating effects of task duration, which further uncover the mechanism behind task reward and performance, that is, the quantity and quality of task contributors.
Assessing contextual factors for sustainable development: a case study of LEED-certified projects in Jordan
Authors: Ebbini Genell Wells , Al-Assaf Adel 
Published: June 2022

PurposeThe researchers analyzed factors affecting the adoption of the Leadership in Energy and Environmental Design (LEED) green-building certification system in Jordan, including financial performance of certified projects along with broader barriers that may impact developers’ interest in LEED.Design/methodology/approachThe authors first reviewed online data for all LEED registered and certified projects in Jordan, recruited LEED-certified project stakeholders, collected documents related to LEED projects and conducted LEED category credit summaries, financial cost-benefit analyses and spot-checking reported values in local markets. The authors then visited projects sites and interviewed various project stakeholders to understand better stakeholders' decision-making processes concerning LEED and relevant factors (financial, branding, cultural, political, etc.).FindingsObtaining LEED certification in Jordan was financially feasible as evinced in both the quantitative analysis and interviews. However, the authors found that there was very limited interest in LEED among Jordanian developers. Barriers included widespread cynicism toward green building concepts as well as a lack of local expertise in installing and maintaining green technologies. To overcome these barriers, the authors recommend that green building initiatives place a greater emphasis on education and public-promotion activities.Research limitations/implicationsThe research data were limited to projects that had successfully achieved LEED certification. Broader qualitative research conducted across the Jordanian building community could provide additional insights, but such an investigation is beyond the scope of the current study.Originality/valueThe complexity of adapting a Western green building standard (LEED) to a non-Western context is discussed in detail. The findings suggest that understanding regional development challenges, local markets and cultural differences is vital for successfully implementing green building certification systems.
Development of cakes with almond baru flour: chemical composition and its correlations with texture profile analysis
Authors: Ramos Ana Flávia , Mendes Gabriela da Rocha Lemos , Cruz Renato Souza , Silva Fabiane Neves , Camilloto Geany Peruch , de Souza Handray Fernandes , Lima Juliana Pinto de , Paiva Caroline Liboreiro , Brandi Igor VIana 
Published: June 2022

PurposeBaru is a fruit of the Brazilian Cerrado, important to the local economy and social culture. The use in food formulation is due to it being highly available and its nutritional profile; however, the changes in the texture of products are still unclear. In this study, an experimental design was conducted to develop cakes by replacing different levels of wheat flour with baru nut flour, and comparing them.Design/methodology/approachCakes were developed with 20, 40, 60, and 80 replacement of wheat flour by baru nut flour and compared with standard formulation (100% wheat flour). The physical-chemistry composition of the flour, nutritional composition of the cakes and texture profile analysis were evaluated.FindingsBaru nut flour showed greater protein, lipids, and ash than wheat flour, and this result was also observed in the cakes developed. The cake with 80% of almond baru flour showed an increase of 60.3% in protein. The texture profile analysis showed that the addition of baru nut flour affected the texture of products. The cakes showed more hardness and chewiness; however, the springiness and cohesiveness were decreased.Originality/valueThe findings of this study demonstrate that baru nut flour is a great option as a new ingredient for bakery products, due the increase of protein. This is the first study analyzing the effect of baru nut flour addition on the texture profile of bakery products. These results can orientate future studies, especially sensory assays.
Workaholism and work–family conflict: a moderated mediation model of psychological detachment from work and family-supportive supervisor behavior
Authors: Chang Po-Chien , Gao Xiaoxiao , Wu Ting , Lin Ying-Yin 
Published: June 2022

Purpose
The purpose of this study is to examine the effects of the workaholism on work–family conflict via the mediator of psychological detachment from work and the moderator of family-supportive supervisor behavior.

Design/methodology/approach
To avoid common method bias, the authors adopted a three-wave data collection with a one-month lagged design. A total of 500 questionnaires were distributed and 322 usable questionnaires were collected. The PROCESS macro for SPSS was applied to test the hypothesized relationships.

Findings
Findings demonstrated that workaholism is positively related to work–family conflict; psychological detachment from work mediates the relationship between workaholism and work–family conflict. Moreover, family-supportive supervisor behavior moderates the relationship between workaholism and work–family conflict and between workaholism and psychological detachment from work, respectively. Finally, family-supportive supervisor behavior moderates the indirect effect of workaholism and work–family conflict via psychological detachment from work, such that the indirect effect was weaker when family-supportive supervisor behavior was high.

Practical implications
The study suggests that it is necessary for organizations to be responsible for employee well-being in different domains because the impact of workaholism on physical and mental health may bring unexpected consequences because of the lack of recovery and the loss of resources. This study not only shows the importance for individuals to look for ways to disengage from workplace but addresses the significance of supervisory support from organizational aspects.

Originality/value
This study includes psychological detachment from work as mediator and family-supportive supervisor behavior as moderator to investigate the moderated mediation relationship in the current highly demanding workplace. By applying conservation of resource and role scarcity hypothesis regarding individual resource allocation, the results may shed lights on facilitating individuals distancing from obsessively and excessively working mentality and behaviors that further lessen incompatibility in both work and family domains.
Location optimization of emergency medical facilities for public health emergencies in megacities based on genetic algorithm
Authors: Liu Jingkuang , Li Yuqing , Li Ying , Zibo Chen , Lian Xiaotong , Zhang Yingyi 
Published: June 2022

PurposeThe purpose of this study is to discuss the principles and factors that influence the site selection of emergency medical facilities for public health emergencies. This paper discusses the selection of the best facilities from the available facilities, proposes the capacity of new facilities, presents a logistic regression model and establishes a site selection model for emergency medical facilities for public health emergencies in megacities.Design/methodology/approachUsing Guangzhou City as the research object, seven alternative facility points and the points' capacities were preset. Nine demand points were determined, and two facility locations were selected using genetic algorithms (GAs) in MATLAB for programing simulation and operational analysis.FindingsComparing the results of the improved GA, the results show that the improved model has fewer evolutionary generations and a faster operation speed, and that the model outperforms the traditional P-center model. The GA provides a theoretical foundation for determining the construction location of emergency medical facilities in megacities in the event of a public health emergency.Research limitations/implicationsFirst, in this case study, there is no scientific assessment of the establishment of the capacity of the facility point, but that is a subjective method based on the assumption of the capacity of the surrounding existing hospitals. Second, because this is a theoretical analysis, the model developed in this study does not consider the actual driving speed and driving distance, but the speed of the unified average driving distance and the driving distance to take the average of multiple distances.Practical implicationsThe results show that the method increases the selection space of decision-makers, provides them with stable technical support, helps them quickly determine the location of emergency medical facilities to respond to disaster relief work and provides better action plans for decision makers.Social implicationsThe results show that the algorithm performs well, which verifies the applicability of this model. When the solution results of the improved GA are compared, the results show that the improved model has fewer evolutionary generations, faster operation speed and better model than the intermediate model GA. This model can more successfully find the optimal location decision scheme, making that more suitable for the location problem of megacities in the case of public health emergencies.Originality/valueThe research findings provide a theoretical and decision-making basis for the location of government emergency medical facilities, as well as guidance for enterprises constructing emergency medical facilities.
The antecedents of customer mistreatment: a meta-analytic review
Authors: Liu Pei , Ma Yu , Li Xin , Peng Caodie , Li Yaoqi 
Published: June 2022

PurposeFrontline service employees are often subjected to customer mistreatment and considerable studies have tested outcomes of customer mistreatment. However, the importance of its antecedents is particularly underestimated. This meta-analytic paper aims to develop an overarching framework that identifies the antecedents of customer mistreatment as well as potential boundary conditions to account for observed variations reported in extant studies.Design/methodology/approachComprehensive electronic and manual searches were performed to retrieve relevant studies on customer mistreatment, which yielded 125 articles, including 141 independent samples. Altogether, these studies included 40,151 participants. The data were analyzed through random-effect meta-analytic methods in R using the psychmeta package.FindingsThree types of antecedents were identified. In particular, regarding employees’ demographic characteristics, age was found to be negatively correlated with customer mistreatment. Employees’ personality traits such as agreeableness, conscientiousness, positive affectivity, emotion regulation ability and self-efficacy were found to be negatively correlated with customer mistreatment, while neuroticism and negative affectivity were positively correlated with customer mistreatment. In terms of contextual factors, perceived social support and service climate were negatively related to customer mistreatment, whereas job demands were positively related to customer mistreatment. Moreover, the power distance culture and types of service industries moderated some relationships.Originality/valueThis meta-analytic research, drawing upon the perpetrator predation framework, proposed a new and comprehensive framework to explain why customer mistreatment occurs. It not only promoted the advancement of literature on customer mistreatment but also provided effective and targeted guidance for helping frontline service employees reduce such negative experience.
Towards an evolutionary view of innovation diffusion in open innovation ecosystems
Authors: Xiong Bingqing , Kuan Lim Eric Tze , Tan Chee-Wee , Zhao Zheng , Yu Yugang 
Published: June 2022

PurposeThe concept of open innovation has captured the attention of both academics and practitioners alike. However, there is a dearth of research on how innovations can be diffused within open innovation ecosystems, a critical condition for the sustainability of such ecosystems. In this regard, the study advances a research agenda for guiding future inquiries into innovation diffusion within open innovation ecosystems.Design/methodology/approachBased on a systematic review of the extant literature on open innovation, this article identifies knowledge gaps in innovation diffusion, along with recommendations for bridging these gaps in the future. The study advocates that future research should consider not only innovation generation processes, but also innovation diffusion processes, especially in light of the growing application of open innovation in the context of digital goods and services.FindingsSubscribing to an evolutionary view of innovation diffusion, the article draws on a five-phase framework – knowledge, persuasion, decision, implementation, and confirmation – to illustrate the roles played by three distinct yet interconnected parties (i.e. platforms, complementors, and individuals) within open innovation ecosystems as well as the research opportunities it brings.Originality/valueThe article examines the critical, yet underexplored role of innovation diffusion in sustaining open innovation ecosystems and outlines potential research avenues that can contribute to growing the understanding of the innovation diffusion process.