bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic and clinicopathologic significance of circZFR in multiple human cancers
Authors: Liu Zhongyue , Zhang Wenchao , Tu Chao , Li Wenyi , Qi Lin , Zhang Zhiming , Wan Lu , Yang Zhimin , Ren Xiaolei , Li Zhihong 
Published: April 2022

Abstract
Abnormally expressed in diverse cancers, circZFR has been correlated with clinical outcomes of cancer patients. Aim of this meta-analysis was to elucidate the prognostic role for circZFR in multiple human malignancies. Literature retrieval was conducted by systematically searching on Pubmed, Web of Science, and Cochrane library up to December 2, 2021. Hazard ratios (HRs) or odds ratios (ORs) with 95% confidence intervals (CIs) were pooled to evaluate the association between circZFR expression and overall survival (OS). The reliability of the pooled results was assessed by sensitivity analysis and the publication bias was measured by Begg’s and Egger’s test. A total of seventeen studies comprising 1098 patients were enrolled in this meta-analysis. Results demonstrated that high circZFR expression was correlated with an unfavorable OS (HR = 2.14, 95%CI: 1.74, 2.64). High circZFR expression predicted larger tumor size (OR = 2.79, 95%CI: 1.52, 5.12), advanced clinical stage (OR = 3.38, 95% CI: 1.49, 7.65), tendentiousness of lymph node metastasis (OR = 3.08, 95% CI: 2.01, 4.71) and malignant grade (OR = 3.18, 95%CI: 1.09, 9.30), but not related to age, gender and distant metastasis (DM). High circZFR expression was associated with unfavorable OS and clinicopathologic parameters including tumor size, clinical stage, LNM and histology grade, implicating a promising prognostic factor in cancers.
Apocynin exerts neuroprotective effects in fumonisin b1 induced neurotoxicity via attenuation of oxidative stress and apoptosis
Authors: Krupashree K , Rachitha P 
Published: April 2022

Abstract
The mycotoxin fumonisin b1 (Fb1) is produced by fusarium verticillioides, which commonly infects corn and other agricultural products. Fb1 found to be a source of vast range of toxic effects like hepatotoxicity, neurotoxicity and carcinogenicity in a number of animal species. However, the information available currently regarding neurotoxic effects exerted by Fb1 is scanty. Hence, the present study was aimed to evaluate the neurotoxic effects of Fb1 and the possible mechanisms of toxicity in mice and as well as the role of cytotoxic, oxidative stress and apoptosis in neuroblastoma (SH-SY5Y) cell line. In this context, the toxicity of Fb1 was examined in male albino mice. Apocynin with 25,50,100mg/kg b/wt was pretreated for 7 days oral administration. Fb1 6.75 mg/kg b/wt was injected subcutaneously for 5 days. A significant elevation of 5-HT was observed in mice treated with Fb1 in whole brain showed biogenic amines may reflect Fb1 neurotoxicity which was attenuated by pretreatment of apocynin. Fb1 treatment increased oxidative damage in the brain, as evidenced by a decrease in GSH level along with significant increases in ROS, lipid peroxidation, protein carbonyl. Apocynin supplementation significantly ameliorated these biological parameter changes. In addition, apocynin pretreatment normalized the degenerative changes in histology studies. The cytotoxicity of Fb1 was evaluated by MTT and LDH assay showed that, Fb1 induced dose-dependent cell death in SH-SY5Y cells and IC50 value was determined as 150µM. Fb1 showed elevated reactive oxygen species, depolarized mitochondrial membrane potential and TEM observation showed vacuolation in SH-SY5Y cells. Further, apoptotic changes revealed in DAPI staining and DNA damage in comet assay. To overcome these toxicological effects, apocynin was pretreated followed by Fb1 exposure for 24 h. Pretreatment with apocynin resulted in significant increase in cell viability, restored membrane integrity, reactive oxygen species level was maintained and neutralized apoptotic changes. The protein expression CAT, GPx downregulated with Fb1 treatment and apoptotic markers caspase-3 and caspase-8 was upregulated. Pretretment with apocynin restored the antioxidant enzymes and overexpressed apocynin markers also altered. Collectively, these results suggest that ROS is the main upstream signal leading to increased Fb1 mediated neurotoxicity in mice and SH-SY5Y cells. Use of an antioxidant apocynin reversed the toxin-induced oxidative stress and apoptosis by its antioxidant potency.
Stability is essential for insecticidal activity of Vip3Aa toxin against Spodoptera exigua
Authors: Fu Bai-Wen , Xu Lian , Zheng Mei-Xia , Chen Qing-Xi , Shi Yan , Zhu Yu-Jing 
Published: April 2022

Abstract
Vegetative insecticidal proteins 3A (Vip3A) were important insecticidal proteins for control of lepidopteran pests. Previous study demonstrated that Vip3Aa and Vip3Ad showed significant difference in insecticidal activities against Spodoptera exigua, while the molecular mechanism remained ambiguous. Here we demonstrated that the difference in insecticidal activities between Vip3Aa and Vip3Ad might be caused by the difference in stability of Vip3Aa and Vip3Ad in S. exigua midgut protease. Vip3Aa was quite stable while Vip3Ad could be further degraded. Molecular dynamics simulation revealed that Vip3Aa was more stable than Vip3Ad, with smaller RMSD and RMSF value. Amino acid sequence alignment indicated that three were three extra prolines (P591, P605 and P779) located on Vip3Aa. We further identified that residue P591 played a crucial role on stability and insecticidal activity of Vip3Aa. Taken together, our study demonstrated that the stability was essential for the insecticidal activity of Vip3A toxins, which might provide new insight into the action mode of Vip3A toxins and contribute to the design Vip3A variants with improved stability and insecticidal activity.
SARS-CoV-2-reactive IFN-γ CD4 + and CD8 + T cells, clinical severity biomarkers and mortality in unvaccinated critically ill COVID-19 patients
Authors: Olea Beatriz , Albert Eliseo , Giménez Estela , Torres Ignacio , Amat Paula , Remigia María José , Alberola Juan , Carbonell Nieves , Ferreres José , Blasco María Luisa , Navarro David 
Published: April 2022

Abstract
Objectives: We examined the relationship between peripheral blood levels of SARS-CoV-2 S1/M-reactive IFN-γ-producing CD4+ and CD8+ T cells, serum levels of biomarkers of clinical severity and mortality in critically ill COVID-19 patients. Methods: Immune responses were monitored in 71 non-consecutive patients (49 male and 22 female; median age, 65 years) by whole-blood flow cytometry (326 specimens). SARS-CoV-2 RNA loads in paired tracheal aspirates [TA] (n=147) were available from 54 patients. Serum levels of interleukin-6, ferritin, D-Dimer, lactose dehydrogenase and C-reactive protein in paired sera were known. Results: SARS-CoV-2 T cells (either CD4+, CD8+ or both) were detectable in 70 patients. SARS-CoV-2 IFN-γ CD4+ T-cell responses were documented more frequently than their CD8+ counterparts (62 vs. 56 patients) and were of greater magnitude overall. Detectable SARS-CoV-2 S1/M-reactive CD8+ and CD4+ T-cell responses were associated with higher SARS-CoV-2 RNA loads in TA. SARS-CoV-2 RNA load in TA decreased over time, irrespective of the dynamics of SARS-CoV-2-reactive CD8+ and CD4+ T cells. Conclusion: Enumeration of peripheral blood levels of SARS-CoV-2-S1/M-reactive IFN-γ CD4+ and CD8+ T cells does not predict viral clearance from the lower respiratory tract or poor clinical outcomes in critically ill COVID-19 patients.
Microglial Response in Triggering Receptor Expressed on Myeloid Cells 2 (Trem2) Knock-out Mice After Systemic Stimulation With Escherichia Coli
Authors: Hoogland Inge C.M. , Yik Jutka , Westhoff Dunja , Engelen-Lee Joo-Yeon , Seron Merche Valls , Man Wing-Kit , Houben-Weerts Judith H.P.M. , Tanck Michael W. , Westerloo David J. van , Poll Tom van der , Gool Willem A. van , de Beek Diederik van 
Published: April 2022

Abstract
Background: Systemic infection is an important risk factor for delirium, associated with neurodegeneration and subsequent cognitive impairment in older people. Microglial cell response is a known key player in this process and we hypothesize that the triggering receptor expressed on myeloid cells 2 (TREM2) plays an important role in the regulation of this response. Methods: 8- to 10-week old male wild-type (WT) and TREM2 knock-out (Trem2-/-) mice were intraperitoneally inoculated with live Escherichia coli (E. coli) or saline. After inoculation, all mice were treated with ceftriaxone (an antimicrobial drug) at 12 and 24 hours and were sacrificed after 2 and 3 days. Microglial response was determined by immunohistochemical staining with an ionized calcium-binding adaptor molecule 1 (Iba-1) antibody and flow cytometry. mRNA expression of pro- and anti-inflammatory mediators was measured to quantify the inflammatory response.Results: We observed increased Iba-1 positive cells number in thalamus of Trem2-/- mice at 3d after inoculation compared to WT mice (mean 120 cell/mm2 [SD 8] vs. 105 cell/mm2 [SD 11]; p=0.03). Flow cytometry showed no differences in forward scatter or expression of CD11b, CD45 and CD14 between WT and Trem2-/- mice. The brain mRNA expression levels of tumor necrosis factor alpha (TNF-α) of Trem2-/- mice at 2d were higher compared to WT mice (p=0.003). Higher mRNA expression of interleukin 1 beta (IL-1β), Iba-1, CD11b and mitogen-activated protein kinase 1 (MAPK-1) was found in brain of WT mice at 2d compared to Trem2-/- mice (respectively p=0.02; p=0.001; p=0.03 and p=0.02). In spleen there were no differences in inflammatory mediators, between WT and Trem2-/- mice.Interpretation: Although the loss of function of TREM2 during systemic infection led to an increased number of activated microglia in the thalamus, we did not observe a consistent increase in expression of inflammatory genes in the brain. The role of TREM2 in the neuro-inflammatory response following systemic infection therefore appears to be limited.
Investigation on pore structure regulation of activated carbon derived from sargassum and its application in supercapacitor
Authors: Li Shijie , Tan Xiaopeng , Li Hui , Gao Yan , Wang Qian , Li Guoning , Guo Min 
Published: April 2022

Abstract
In order to realize the effective regulation of the pore structure of activated carbon and optimize its pore structure properties as electrode material, the effects of activation temperature, activation time and impregnation ratio on the specific surface area, total pore volume and average pore diameter of activated carbon are studied by orthogonal experiment with sargassum as raw material. In addition, the electrochemical properties of sargassum-based activated carbon (SAC) and the relationship between the gravimetric capacitance and specific surface area of SAC are also studied. The SACs prepared under all conditions have high specific surface area (≥2227 m2 g-1) and developed pore structure, in which the micropore diameter is mainly concentrated in 0.4~0.8 nm, the mesopore diameter is mainly concentrated in 2~4 nm, and the micropore content is much higher than that of the mesopore. In the activation process, the impregnation ratio has the greatest effect on the specific surface area of SAC, the activation temperature and impregnation ratio have significant effect on the total pore volume of SAC, and the regulation of the average pore diameter of SAC is mainly realized by adjusting the activation temperature. The SACs exhibit typical electric double layer capacitance performances on supercapacitors, delivering superior gravimetric capacitance of 237.3 F g-1 in 6 mol L-1 KOH electrolyte system at current density of 0.5 A g-1 and excellent cycling stability of capacitance retention of 92% after 10000 cycles. A good linear relationship between gravimetric capacitance and specific surface area of SAC is observed.
Inverted Acetabular Labrum Causes Insufficiency-type Stress Fracture of the Femoral Head: A Biomechanical Study
Authors: Fukui Kiyokazu , Chen Mingliang , Kaneuji Ayumi , Takahishi Eiji , Takano Masahiro , Miyakawa Hiroyasu , Hirosaki Kenichi , Kawahara Norio 
Published: April 2022

Abstract
Purpose: Subchondral insufficiency-type fracture is associated with rapidly progressive osteoarthritis of the hip, but are these fractures caused by a single load event such as an injury from a fall or by stress (fatigue) from repeated loading during the activities of daily life? Our biomechanical study applied repeated loading to a simulated osteoporotic bone model to investigate the relationship between an inverted acetabular labrum and the incidence of subchondral insufficiency-type fracture of the femoral head.Methods: We performed fatigue load testing on 15 osteoporotic composite femurs, applying 2000 N at 1 Hz, 1 million times or to failure, on 5 composite femurs without silicone rubber and 10 with 10x5 mm silicone rubber. Silicone rubber was inserted as a surrogate for inversion of the acetabular labrum between the acetabulum and femoral head.Results: No breakage was noted in the 5 specimens without silicone rubber, even after the application of 1 million individual loads at 1 Hz. Fractures appeared in the anterior femoral head in all 10 specimens with 10×5 mm silicone rubber, and mean number of loads to fracture was 6389. Conclusion: Our research selected an osteoporotic bone model to investigate the effects of loading tests that were designed to mimic typical repeated loads in daily activities, using silicone rubber as a surrogate for inversion of the acetabular labrum and resulting in the development of insufficiency-type stress fracture on the femoral head.Concept diagram or graphical abstract
Performance analysis of the BDSBAS-B1C message in trial operation stage
Authors: Xin Jie , Guo Rui , Tian Yijun , Chen Jinping , Cao Yueling , Liu Yuchen , Li Xiaojie , Wang Dongxia , Cai Hongliang 
Published: April 2022

Abstract
BeiDou Satellite-based Augmentation System (BDSBAS) has come into the trial operation stage since July, 2020. To evaluate the characteristic of the augmentation message in BDSBAS-B1C signal, the effectiveness of the message content was firstly analyzed, and then the validity of the broadcasting strategy was estimated. Finally, the accuracy of the user equivalent ranging error (UERE) and the single frequency (SF) positioning error (PE) with different correction parameters in BDSBAS-B1C message were evaluated. Based on the above analysis, the effectiveness of the augmentation message was preliminarily verified with the results showing that: 1) the BDSBAS-B1C message type, information content and update interval have basically met the international standard; 2) the accuracy of the UERE obtained with the augmentation message had an obvious improvement in contrast to that of the UERE obtained with the usual navigation message of the GPS satellites, and the ionospheric delay was one of the important factors which affected the accuracy of the UERE; 3) the positioning accuracy obtained with the augmentation message was also improved, and the improvement was more obvious in the service areas with high availability of the ionospheric parameters.
Evaluation of micro-textured tool performance in Ultrasonic Elliptical Vibration-assisted turning of 304 stainless steel
Authors: Usman Mustapha Mukhtar , Zou Ping , Yang Zhenyu , Lin Tianyu , Muhammad Isyaku 
Published: April 2022

Abstract
The application of micro-textured cutting tools in various machining operations has been widely recommended as an efficient method for improving cutting performance. However, the realm of micro-textured tool utilization is rarely found in non-conventional cutting processes. This paper investigates the performance capability of a rake-faced micro-textured tool in Ultrasonic elliptical vibration cutting (UEVC) which is a typical non-conventional cutting technique. A comparative experimental turning of 304 stainless steel with the micro-textured tool was performed under UEVC and Conventional Cutting (CC) while employing variable cutting parameters as governing factors. The cutting performance of the micro-textured tool was measured in terms of Cutting forces, Surface topography, Chip morphology, and Wear mechanism. The experimental findings revealed that the micro-textured tool outperformed the non-textured tool (NTT) in the overall machining-related outputs and the combined effect of utilizing the UEVC mechanism and cutting tool texturing techniques can significantly enhance the remarkable machining benefits of UEVC.
Silencing plasmacytoma variant translocation 1 promotes the anticancer activity of sorafenib in papillary thyroid carcinoma cells
Authors: Ya Wen , Li Hong-yi , Zhai Zi-Xin , Yuan Si-Jie , Xin Yu-Wei , Yan Xiao-Hui , Hao Yan-Hong , Fan Wen-Wen , Liu Li-Ping 
Published: April 2022

Abstract
Background: Sorafenib is an effective treatment for radioiodine (RAI)-refractory differentiated thyroid carcinoma (DTC), but adverse events limit its use. We investigated the synergistic anticancer effects of silencing lncRNA-PVT1 and sorafenib in papillary thyroid carcinoma (PTC) cell lines.Methods: B-CPAP, TPC-1, IHH-4 and K1 papillary thyroid carcinoma cell lines were used. A small interfering RNA (siRNA)-PVT1 sequence was used to silence lncRNA-PVT1 expression. Cell proliferation, migration and invasion, apoptosis, and cell cycle assays were conducted following PVT1 silencing and/or sorafenib treatment.Results: Sorafenib had inhibitory effects on PTC cell lines, and the cell survival rate decreased with increasing concentrations. LncRNA-PVT1 knockdown combined with 9 μM sorafenib in B-CPAP cells and 13 μM sorafenib in TPC-1 cells synergistically reduced the number of colonies formed, reduced invasion and migration, and promoted cell apoptosis.Conclusion: Sorafenib had inhibitory effects on TPC-1, B-CPAP, IHH4, and K1 cells, and lncRNA-PVT1 knockdown combined with sorafenib in B-CPAP and TPC-1 cells synergistically reduced the number of colonies formed, reduced invasion and migration, and promoted cell apoptosis.
The Change Assessment and Analysis of Impacts From Land Use Policies on Wetland Ecosystems Based on Multitemporal Satellite Image Data: a Case Study in Dong Rui Commune, Tien Yen District, Quang Ninh Province, Vietnam
Authors: Trung Dung Ngo , Dang Hoi Nguyen , Viet Huu Nguyen Huu 
Published: April 2022

Abstract
Wetlands are one of the most important ecosystems on Earth and are an important habitat for many species of animals and plants, for disaster mitigation, for improving environmental quality, for storing carbon, and for responding to climate change. However, they are sensitive ecosystems that have been strongly affected by human economic activities, including aquaculture. In this study, we used multitemporal satellite imagery integrated with a verified field survey method to map the mantle of the wetland ecosystem in the Dong Rui commune, Tien Yen district, Quang Ninh Province, Vietnam at 5 times for 4 periods from 1975 to 2020 with high accuracy (overall accuracy = 92,3%, Kappa = 0,91). The results of the study showed that from 1975 to 2000, the area of mangrove forests declined sharply (nearly 2,000 hectares), mainly due to policies on the development and conversion of land use purposes. From 2000 to 2020, the area of mangrove forests was gradually restored, and the area of aquaculture land shrank. Human impacts, especially the effects of local economic development, conservation and development policies, have directly affected and are the main cause of continuous change in each very short period of the Dong Rui commune wetland ecosystem. Assessing the fluctuations of multitemporal wetland ecosystems and analysing the impact of development policies is the basis for planning, conservation strategies, and sustainable development of wetland ecosystems associated with the livelihoods of the council.
Timothy Syndrome and Torsades De Pointes in a Newborn - Case Report
Authors: Lucas Eliane , Bravo-Valenzuela Nathalie Jeanne Magioli , Loureiro Talita Nolasco , Madruga Anette Presciliana Boabaid , Sacramento Lívia Maria Andrade , Lima Camille Caetano 
Published: April 2022

Abstract
Timothy syndrome (TS) is a rare multisystem disease has a characterized association of long QT syndrome (LQTS) type-8 with the congenital cardiac and extracardiac malformations (neurological and dysmorphic facial features, syndactyly). It is caused by heterozygous mutations in the CACNA 1C gene, which participates in decoding the calcium channels. In this study, we describe a case of TS, which has systemic phenotypic characteristics associated with a long QT interval on the electrocardiogram.. The LQTS predisposes to a characteristic life-threatening ventricular arrhythmia known as Torsades de pointes or "twisting of the points". In this scenario, the authors describes the main clinical characteristics in a case of TS focusing on improve the ability of early diagnosis and better management of LTQS type-8.
Choroid Thickness Changes Analysis in Adolescents with Different Types of High Myopia
Authors: Ji Yanyan , Chen Song , LI Zhong-qing , Wang Jia , Cui Ying-chun , Lu Shi-lin 
Published: April 2022

Abstract
AIM: To evaluate the changes of choroid thickness in adolescence with different types of high myopia. METHODS: From January to November 2021, 134 eyes of 78 adolescents with myopia in Liaocheng Aier Eye Hospital were collected and analyzed. According to the Mean Spherical Equivalent(MSE) and Corneal Curvature(CC), subjects are divided into mild myopia or emmetical eye group (ME group), high myopia group (HM group), occult high myopia group(OHM group) and super high myopia group(SHM group). The macular foveal choroidal thickness(CT) of the four groups was analyzed by Using Zeiss frequecy domain optical coherence tomography (Cirrus HD OCT), acquire horizontal and vertical cross-sectional images of macular. The CT values were measured manually subfoveal choroidal thickness(SFCT) and 1mm,3mm from the fovea at 9 sites, including the superior, nasal, inferior, temporal. The same operator completed the measurement 3 times ,and took the average numerical value as the final reference data.RESULTS:. There were significant differences in choroid thickness at 1mm and 3mm in macular fovea (P < 0.05). Compared with the ME group, SFCT, 1mm and 3mm in the HM group, OHM group and SHM group were significantly thinner than those in ME group (P < 0.05). Compared with SHM, HM and OHM group were significant differences in CT (P < 0.05). There was no statistical significance in CT of each point in HM group and OHM group (P > 0.05). CONCLUSION: The CT of SHM is significantly lower than that of HM and OHM. Although OHM has a lower degree of myopia, the CT has become significantly thinner, and the degree of choroid thinning is almost the same as that of OHM.
“Rice Stubble: Cotton Fly Waste Composites for Acoustic Applications”
Authors: SINGH MUKESH Kumar , Kishor Kaushal 
Published: April 2022

Abstract

Management of noise in everyday life is critical task to enhance the comfort level in present environment or not to resentfully affectthe external environment. The burning of rice stubble particularly in rice growing Asian countries makes the situation bad to worst. Rice stubble, cotton fly waste were used to manufacture composite material to be used as acoustic panel. Rice stubble powder 60,70 and 80%, cotton fly waste 20,30, and 40% by weight, and polyurethane (PU) resin were used to produce composite for acoustic applications. The composites were tested for pore size, morphology, water absorbability, and noise reduction coefficient mainly. Acceptable level of NRC and other features were achieved. This study will provide a new pathway to use the discarded challenging waste materials to manufacture wealth generating products.