bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recapitulating the life cycle of the global pathogenEntamoebain mice
Authors: Cavazos Carolina Mendoza , Heredia Marienela Y. , Owens Leah A. , Knoll Laura J. 
Published: October 2022

AbstractThere are severalEntamoebaspecies that colonize humans, but onlyEntamoeba histolyticacauses severe disease.E. histolyticais transmitted through the fecal-oral route to colonize the intestinal tract of 50 million people worldwide. The current mouse model to studyE. histolyticaintestinal infection directly delivers the parasite into the surgically exposed cecum, which circumvents the natural route of infection and does not produce infectious cysts. To develop a fecal-oral mouse model, we screened our vivarium for a natural murineEntamoebacolonizer via a pan-EntamoebaPCR targeting the 18S ribosomal gene. We determined that C57BL/6 mice were chronically colonized byEntamoeba muris. This amoeba is closely related toE. histolytica, as determined by 18S sequencing and cross-reactivity with anE. histolytica-specific antibody. In contrast, outbred Swiss Webster (SW) mice were not chronically colonized byE. muris. We orally challenged SW mice with 1×105E. muriscysts and discovered they were susceptible to infection, with peak cyst shedding occurring between 5-7 days post-infection. Most infected SW mice did not lose weight significantly but trended toward decreased weight gain throughout the experiment when compared to mock-infected controls. Infected mice treated with paromomycin, an antibiotic used against non-invasive intestinal disease, do not become colonized byE. muris. Within the intestinal tract,E. murislocalizes exclusively to the cecum and colon. PurifiedE. muriscysts treated with bovine bilein vitroexcyst into mobile, pre-trophozoite stages. Overall, this work describes a novel fecal-oral mouse model for the important global pathogenE. histolytica.ImportanceInfection with parasites from theEntamoebagenus are significantly underreported causes of diarrheal disease that disproportionally impact tropical regions. There are several species ofEntamoebathat infect humans to cause a range of symptoms from asymptomatic colonization of the intestinal tract to invasive disease with dissemination. AllEntamoebaspecies are spread via the fecal-oral route in contaminated food and water. Studying the life cycle ofEntamoeba, from host colonization to infectious fecal cyst production, can provide targets for vaccine and drug development. Because there is not an oral challenge rodent model, we screened for a mouseEntamoebaspecies and identifiedEntamoeba murisas a natural colonizer. We determine the peak of infection after an oral challenge, the efficacy of paromomycin treatment, the intestinal tract localization, and the cues that trigger excystation. This oral infection mouse model will be valuable for the development of novel therapeutic options forEntamoebainfections.
Sex-specific genetic loci linked to early and late onset type 2 diabetes
Authors: Berumen Jaime , Orozco Lorena , Gallardo-Rincón Héctor , Benuto Rosa Elba , Ramos-Martinez Espiridión , Rivas Fernando , García-Ortiz Humberto , Marin-Medina Melissa , Barrera Elizabeth , Juárez-Torres Eligia , Alvarado Silva Anabel , MartÍnez-Juárez Luis Alberto , Lomelín-Gascón Julieta , Montoya Alejandra , Ortega-Montiel Janinne , Alvarez-Hernández Diego-Abelardo , Tapia-Conyer Roberto 
Published: October 2022

AbstractPurposeTo investigate the effect of sex and age on the timing of a type 2 diabetes (T2D) diagnosis and the influence T2D-related genes, parental history of T2D, and obesity on T2D development.MethodsIn this case-control study, 1012 T2D cases and 1008 healthy subjects were selected from the Diabetes in Mexico Study database. Participants were stratified by sex and age at T2D diagnosis (early, ≤45 years; late, ≥46 years). Seventy T2D-associated SNPs were explored and the percentage contribution (R2) of T2D-related genes, parental history of T2D, and obesity (body mass index [BMI] and waist-hip ratio [WHR]) on T2D development was calculated using univariate and multivariate logistic regression models.ResultsT2D-related genes influenced T2D development most in males who were diagnosed early (R2= 23.5%; females diagnosed early, R2= 13.5%; males and females diagnosed late, R2= 11.9% and R2= 7.3%, respectively). With an early diagnosis, insulin production genes were more influential in males (76.0% of R2) whilst peripheral insulin resistance genes were more influential in females (52.3% of R2). With a late diagnosis, insulin production genes from chromosome region 11p15.5 notably influenced males while peripheral insulin resistance and inflammation genes notably influenced females. Influence of parental history was higher among those diagnosed early (males, 19.9%; females, 17.5%) versus late (males, 6.4%; females, 5,3%). Unilateral maternal T2D history was more influential than paternal T2D history. BMI influenced T2D development for all, while WHR exclusively influenced males.ConclusionsThe influence of T2D-related genes, maternal T2D history, and fat distribution on T2D development was greater in males than females.
Single nucleus and spatially resolved intra-tumor subtype heterogeneity in bladder cancer
Authors: Lindskrog Sia V. , Schmøkel Sofie S. , Nordentoft Iver , Lamy Philippe , Knudsen Michael , Prip Frederik , Strandgaard Trine , Jensen Jørgen Bjerggaard , Dyrskjøt Lars 
Published: October 2022

SummaryCurrent transcriptomic classification systems for bladder cancer do not consider the level of intra-tumor subtype heterogeneity. Here we present an investigation of the extent and possible clinical impact of intra-tumor heterogeneity across early and more advanced disease stages of bladder cancer. We performed single nucleus RNA-sequencing of 48 bladder tumors and four of these tumors were additionally analyzed using spatial transcriptomics. Total bulk RNA-sequencing and spatial proteomics data were available from the same tumors for comparison, along with detailed clinical follow-up of the patients. We demonstrate that tumors display varying levels of intra-tumor subtype heterogeneity and show that a higher class 2a weight estimated from bulk RNA-sequencing data is associated with worse outcome in patients with molecular high-risk class 2a tumors. Our results indicate that discrete subtype assignments from bulk RNA-sequencing data may lack biological granularity and continuous class scores could improve clinical risk stratification of patients.HighlightsSingle nucleus RNA-sequencing of tumors from 48 bladder cancer patients.Tumors display varying levels of intra-tumor subtype heterogeneity at single nucleus and bulk tumor level.The level of subtype heterogeneity could be estimated from both single nucleus and bulk RNA-sequencing data with a high concordance between the two.High class 2a weight estimated from bulk RNA-sequencing data is associated with worse outcome in patients with molecular high-risk class 2a tumors.
Bibliometric approach to inclusive entrepreneurship: what has been written in scientific academia?
Authors: Rodrigues Margarida , Silva Rui , Franco Mário , Oliveira Cidália 
Published: October 2022

Purpose
The year 2020 was heavily marked by an unprecedented pandemic affecting society as a whole. However, under-represented groups may have seen their financial and social situation affected differently from other groups. Thus, it was found that in the literature, the term inclusive entrepreneurship, which addresses these issues, was fragmented in view of its similarity and association with social entrepreneurship, inclusive business and sustainability. In this sense, this paper aims to map the scientific knowledge on this topic.

Design/methodology/approach
To fulfil this aim, a systematic literature review was supported by bibliometrics (performance analysis and scientific mapping) and by the use of the software Bibliometrix R and VoSviewer.

Findings
The results obtained show that in the Web of Science, there are 121 documents related to this topic whose content analysis revealed that they are distributed between sustainability, entrepreneurship and inclusive entrepreneurship in the close triple association.

Practical implications
The main contributions of this study are the connection established between the three concepts and the emergence of continuing to develop research on inclusive entrepreneurship, given its binary function: employment generation for disadvantaged groups and inclusive business creation.

Originality/value
The relevance of this bibliometric analysis stands out, providing the positioning of academics on the importance of leveraging emerging research on this topic, not only in poor countries but also in others.
Trade show visitors and key technological trends: from a literature review to a conceptual framework
Authors: Vitali Veronica , Bazzani Claudia , Gimigliano Annamaria , Cristani Marco , Begalli Diego , Menegaz Gloria 
Published: October 2022

Purpose
This study proposes a literature review and, based on the findings, the authors develop a conceptual framework, attempting to explain how technology may influence visitor behavior and eventually trade show performance.

Design/methodology/approach
The present research explores the role of visitors in the trade show context. The analysis specifically focuses on the variables that influence visitors’ participation at business-to-business trade shows and how their satisfaction and perception can be related to exhibition performance. The authors also take into consideration technological trends that prior to COVID-19 pandemics were slowly emerging in the trade show industry.

Findings
The findings highlight a continuity between pre-, at and postexhibition phases. Visitors’ behavior represents a signal of how a trade show is perceived as postexhibition purchases and next visit emerge as signals of an exhibition evaluation in relation to visitors’ perception. Besides being urgent tools for the continuity of the sector due to the pandemics, emerging technological trends can be key elements in understanding visitors’ behavior and in boosting their interest and loyalty toward trade shows.

Originality/value
The paper proposes a conceptual model including top notch and innovative technological trends to improve the understandment of visitors’ behavior. Both practitioners in companies and academics might find the study useful, given the digital uplift generated by the pandemics.
Spiritual leadership and corporate social entrepreneurial orientation: the mediating role of workplace spirituality
Authors: Luu Tien Dung 
Published: October 2022

PurposeThe main aim of this study is to examine a moderated–mediated model empirically to understand whether and how spiritual leadership (SL) affects corporate social entrepreneurial orientation (SEO) with the role of workplace spirituality (WS).Design/methodology/approachThe study adopts a sample of 524 key role employees at 76 four–five-star hotels and tourism firms in Ho Chi Minh City of Vietnam, using a partial least square structural equation model (PLS-SEM).FindingsSL of social entrepreneurs can vigorously promote corporate SEO's formation and development, with WS's mediating role.Originality/valueThis study demonstrates how SL and WS can help organisations achieve higher corporate SEO levels in contradictory empirical evidence and a lack of theoretical framework.
Concordant genetic structures of sika deer and Japanese land leeches suggest that the current range expansion of Haemadipsa japonica is dependent on sika deer migration: A case study from Tochigi Prefecture, Japan
Authors: Morishima Kaori , Fukui Emiko , Aizawa Mineaki 
Published: October 2022

AbstractHaemadipsa japonica is a Japanese land leech that feeds on the blood of host animals, mainly the sika deer (Cervus nippon). The distribution of H. japonica has expanded since the 1990s, possibly due to sika deer population increases. Haemadipsa japonica populations were found in the northern and southern regions of Tochigi Prefecture, central Japan. Previous investigations reported that these populations are genetically differentiated between the north and south, although the sika deer distribution extends throughout the northern and southern regions. We hypothesized that the migration of sika deer might be limited, thus maintaining the two H. japonica genetic groups and restricting its range expansion. We evaluated this hypothesis by investigating the genetic structure of the mitochondrial DNA (mtDNA) and nuclear microsatellites (nSSR) of sika deer samples collected from Tochigi Prefecture and from H. japonica bloodmeal samples (invertebrate‐derived DNA; iDNA). The sika deer mtDNA presented two haplotypes (G‐ and A‐haplotypes) without a clear genetic structure. Conversely, the sika deer nSSR analysis indicated clear genetic differentiation between deer in the northern and southern regions, corresponding to the two H. japonica genetic groups. This indicates that sika deer movement is restricted to narrow regions in Tochigi Prefecture. Therefore, we conclude that the current H. japonica expansion in the prefecture likely depends on the migration of sika deer, only occurring within the northern and southern regions, even though this is driven by sika deer migration.
Responses of Salmonella biofilms to oxidizing biocides: Evidence of spatial clustering
Authors: Guest Kerry , Whalley Thomas , Maillard Jean‐Yves , Artemiou Andreas , Szomolay Barbara , Webber Mark A. 
Published: October 2022

AbstractThe spatial organization of biofilm bacterial communities can be influenced by several factors, including growth conditions and challenge with antimicrobials. Differential survival of clusters of cells within biofilms has been observed. In this work, we present a variety of methods to identify, quantify and statistically analyse clusters of live cells from images of two Salmonella strains with differential biofilm forming capacity exposed to three oxidizing biocides. With a support vector machine approach, we showed spatial separation between the two strains, and, using statistical testing and high‐performance computing (HPC), we determined conditions which possess an inherent cluster structure. Our results indicate that there is a relationship between biocide potency and inherent biofilm formation capacity with the tendency to select for spatial clusters of survivors. There was no relationship between positions of clusters of live or dead cells within stressed biofilms. This work identifies an approach to robustly quantify clusters of physiologically distinct cells within biofilms and suggests work to understand how clusters form and survive is needed.Significance statementControl of biofilm growth remains a major challenge and there is considerable uncertainty about how bacteria respond to disinfection within a biofilm and how clustering of cells impacts survival. We have developed a methodological approach to identify and statistically analyse clusters of surviving cells in biofilms after biocide challenge. This approach can be used to understand bacterial behaviour within biofilms under stress and is widely applicable.
Effet du mobile money sur la résilience des ménages exerçant des activités génératrices de revenus au Cameroun
Authors: Mvogo Gregory , Djouaka Christèle Gladisse Awounang 
Published: October 2022

RésuméPartant d'une enquête réalisée en mai 2020 auprès de 1136 ménages au Cameroun, cet article évalue l'effet du mobile money sur les ménages exerçant des activités génératrices de revenus. Les résultats obtenus, par l'estimation d'un modèle de probabilité linéaire à variables instrumentales, montrent que le mobile money permet aux ménages, pour ce qui est des chocs économiques, de faire face aux tensions de trésorerie liées à leur activité tout en payant les factures et à la pandémie COVID‐19. De plus, concernant les chocs sociaux, le mobile money permet aux ménages d'être résilients face à la mort d'un membre important de la famille et aux différentes sollicitations de la famille. Sur la base de cette embellie de l'effet du mobile money, les offreurs du produit gagneraient à intensifier sa diffusion surtout dans les zones rurales où les coûts de transaction sont encore importants.
When men fight with women (versus other men): Limited offending during disputes
Authors: Felson Richard B. , Berg Mark T. , Rogers Ethan M. , Krajewski Andrew T. 
Published: October 2022

AbstractWhat transpires in a dispute, even a violent dispute, is affected by the tendency for adversaries to engage in “limited offending.” We focus on one restraint: the tendency of men to limit their aggression in their disputes with women. Analyses are based on an incident‐level survey about interpersonal disputes administered to 503 men who are incarcerated and 220 men who had never been incarcerated. Using multinomial and logistic regression models, we examined the extent to which an adversary's gender predicted dispute‐related behaviors. The evidence suggests that the chivalry norm has pervasive effects on the behavior of men during their disputes with women. Men are more likely to engage in remedial actions (e.g., apologies) when their adversary is a woman, as opposed to another man. In addition, men are less likely to make violent threats and engage in physical attacks when their adversary is a woman, even after they have themselves been physically attacked. When men are violent, they are less likely to injure a woman than a man. However, the chivalry norm does not inhibit verbal aggression in these disputes: men are just as likely to engage in verbal attacks and nonviolent threats when the adversary is a woman.
High rodent abundance increases seed removal but decreases scatter‐hoarding and seedling recruitment along an elevational gradient
Authors: YU Fei , ZHANG Linjun , WANG Yang , YI Xianfeng , ZHANG Shuang , MA Jianmin , DONG Zimei , CHEN Guangwen , MA Keming 
Published: October 2022

AbstractThe distributions of small rodents in mountainous environments across different elevations can provide important information regarding the effects of climate change on the dispersal of plant species. However, few studies of oak forest ecosystems have compared the elevational patterns of sympatric rodent diversity, seed dispersal, seed bank, and seedling abundance. Thus, we tested the differences in the seed disperser composition and abundance, seed dispersal, seed bank abundance, and seedling recruitment for Quercus wutaishanica along 10 elevation levels in the Taihang Mountains, China. Our results provide strong evidence that complex asymmetric seed dispersal and seedling regeneration exist along an elevational gradient. The abundance of rodents had a significant negative correlation with the elevation and the seed removal rates peaked and then declined with increasing elevation. The seed removal rates were higher at middle and lower elevations than higher elevations but acorns were predated by 5 species of seed predators at middle and lower elevations, and thus, there was a lower likelihood of recruitment compared with those dropped beneath mother oaks at higher elevations. More importantly, the number of individual seeds in the seed bank and seedlings increased with the elevation, although dispersal services were reduced at sites lacking rodents. As conditional mutualists, the rodents could possibly act as antagonistic seed predators rather than mutualistic seed dispersers at low and middle elevations, thereby resulting in the asymmetric pattern of rodent and seedling abundance with increasing elevation to affect the community assembly and ecosystem functions on a large spatial scale.
Sensory characterization of yellow pea and ground chicken hybrid meat burgers using static and dynamic methodologies
Authors: Barker Sophie , McSweeney Matthew B. 
Published: October 2022

AbstractTo reduce animal protein consumption, new food products need to be created. Furthermore, there is a growing number of consumers who consciously act to reduce their meat consumption. Hybrid meat products (HMP) are food items that combine both plant and animal proteins. The objective of this study was to create a hybrid meat burger (HMB) using yellow pea and chicken and to evaluate the sensory properties of the new product using static (check‐all‐that‐apply [CATA]) and dynamic (temporal check‐all‐that‐apply [TCATA]) methods. Yellow pea flour was added to a chicken burger at 0% (control), 10%, 20%, 30%, and 40%. A sensory trial asked participants (n = 69) to evaluate the HMBs using hedonic scales and CATA. A second sensory trial asked experienced panelists (n = 14) to evaluate the items using TCATA. The addition of the yellow pea flour decreased the liking of the burgers, except for the 10% formulation. The burgers made with higher amounts of yellow pea were associated with off‐flavors (beany and nutty; significantly different from the control) during both CATA and TCATA tasks and detracted from consumers’ liking. Juicy, moist, meaty, salty, and soft attributes increased the consumers' liking. The study identified that the addition of yellow pea to chicken burgers is only acceptable to consumers in small quantities (10%). In addition, the yellow peas contributed off‐flavors and a dry texture that was disliked by the consumers.Practical ApplicationThe environmental impacts of livestock production have created a need to incorporate more plant‐based proteins into consumers' diets to increase sustainability. The market for meat alternatives, including hybrid meat products, is expanding; however, current products do not always meet consumers' expectations. Chicken is the fastest‐growing meat sector in North America, and therefore this study's objective was to determine the sensory properties of a burger made from chicken and pulses (yellow pea). It was found that if 10% of chicken in a burger was substituted with yellow pea, then the sensory properties and consumer liking were not significantly affected.
Connectome‐based predictive modelling of smoking severity in smokers
Authors: Lin Xiao , Zhu Ximei , Zhou Weiran , Zhang Zhibo , Li Peng , Dong Guangheng , Meng Shiqiu , Deng Jiahui , Lu Lin 
Published: October 2022

AbstractThe functional connectivity within and between networks could provide a framework to characterize the neurobiological mechanism of nicotine addiction. This study examined the brain regions that were functionally connected in response to smoking cues and established the brain–behaviour relationships in smokers. Sixty‐seven male smokers were enrolled and scanned while performing the cue‐reactivity and Stroop task. A whole‐brain analysis approach, connectome‐based predictive modelling (CPM), was conducted on the data from the cue‐reactivity task to identify the networks that could predict the smoking severity with the Shen atlas as templates. Then, the brain–behaviour relationships were verified in a different brain state (Stroop task). CPM identified the smoking severity‐related network, as indicated by a significant correlation between predicted and actual smoking severity scores (r = 0.31, p = 0.02). Identified networks mainly involved the canonical networks implicated in the reward process (motor/sensory network and salience network) and executive control (frontoparietal network). Network strength in the Stroop task marginally significantly predicted smoking severity scores (r = 0.23, p = 0.06), partially replicating the brain–behaviour relationship. The CPM results identified the whole‐brain neural network related to smoking severity, which was cross‐validated by the AAL and Shen atlas. These findings contribute to more profound insights into neural substrates underlying the smoking severity.