bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shedding of Coxiella Burnetii in Milk of Dairy Cattle and Evidence of Q Fever in Domestic Ruminants with Emphasis on Abortion Cases in Latvia
Authors: Grantiņa-Ieviņa Lelde , Šteingolde Žanete , Boikmanis Guntis , Laizāne Liene , Ringa-Ošleja Guna , Bubula Ilga , Sergejeva Maija , Mališevs Artjoms , Ķibilds Juris , Cvetkova Svetlana , Bergšpica Ieva , Eglīte Lāsma , Cīrule Dina , Kovaļenko Kaspars , Antāne Vita , Bērziņš Aivars 
Published: June 2022

Abstract
Q-fever is widespread globally. The goal of this study was to estimate the prevalence of Q-fever infection in Latvia among dairy cattle in 2018–2020, in comparison with that in 2012–2015. The shedding of Coxiella burnetii DNA in milk was assessed. Screening of blood samples of abortion cases for the presence of antibodies against Q-fever and testing of aborted fetuses for C. burnetii DNA were also carried out. Additionally, serum samples from clinically healthy cattle, sheep and goat were included. Overall, 18.34% of milk samples were positive, representing 11.02% of all tested sheds. In total, 20.62% of serum samples from the cattle that suffered abortions were positive or suspicious, representing 12.63% of all sheds. Only 3.33% of serum samples from clinically healthy cattle and 3.42% from sheep and goat were positive. The highest proportion of serologically positive and suspicious samples was observed in cattle that suffered abortion with age from three to nine years, and in the first and third gestation period. One dominant genotype of C. burnetii (MST61) was detected. Ruminant import from abroad was identified as a significant risk factor, as well as the dairy cattle population density.
Assessment of iPBS Primers Potential to Be Used in Genetic Diversity Studies of Wild Cloudberry (Rubus Chamaemorus L.) Populations
Authors: Krasņevska Nikole , Miķelsone Andra , Kruchonok Alesia , Rashal Isaak , Butkauskas Dalius , Grauda Dace 
Published: June 2022

Abstract
Inter-primer binding site (iPBS) molecular retrotransposon-based markers are a universal and simple tool to estimate genetic diversity. The goal of this study was to select highly informative iPBS primers to be used in studies of intraspecific genetic diversity of cloudberry (Rubus chamaemorus L.), a common plant species of the boreal zone, associated with raised bog habitats and transitional bogs. Initially, 75 iPBS primers were screened and three of the most informative primers (indicated by numbers 2298, 2277, and 2229) were selected based on the highest number of polymorphic bands and PIC (polymorphic information content) values. Electrophoretic analysis revealed 34 bands amplified with primer 2229, 25 bands with primer 2298, and 22 bands with primer 2277. The percentage of polymorphic loci ranged from 90.9 to 97.1% and PIC values ranged from 0.4885 to 0.4965, indicating potential of the selected primers to be applied in population genetic studies of R. chamaemorus.
Influence of the Housing System on Sperm Productivity and Reproductive Capacity of Rabbits
Authors: Kotsiubenko Anna A. , Gill Michael I. , Kotsiubenko Vladimir I. , Petrova Elena I. 
Published: June 2022

Abstract
The influence of the housing system on sperm productivity and reproductive capacity of rabbits was studied. Indicators like ejaculate volume, sperm density, activity, and concentration, as well as the percentage of fertility were measured for silver breed rabbits of three coloured lines housed in aggregates “Rabbitax-8” and in a modular rabbit house developed by Mykolayiv National Agrarian University. The highest male sperm productivity and reproductive capacity were found for rabbits housed in aggregates “Rabbitax-8”.
Insertion in Constructed Normal Numbers
Authors: Becher Verónica 
Published: June 2022

Abstract
Defined by Borel, a real number is normal to an integer base b ≥ 2 if in its base-b expansion every block of digits occurs with the same limiting frequency as every other block of the same length. We consider the problem of insertion in constructed base-b normal expansions to obtain normality to base (b + 1).
ANALISIS KUALITAS AIR DAN STRATEGI PENGENDALIAN PENCEMARAN DI DANAU BATUR MENGGUNAKAN ANALISIS FORCE-FIELD
Authors: Ayu Septiani Ni Komang , Budiarsa Suyasa I Wayan , Rai I Nyoman 
Published: June 2022

Batur is the largest lake in Bali which has the potential to experience pollution due to the high activity in the body and the surrounding environment. This condition indicates the need for serious management efforts to maintain water quality. This study aims at analysing the water quality, status of the water quality of Lake Batur and also formulate a strategy for controlling water pollution in Lake Batur. The water sample analysis method was carried out in two ways, namely in situ method and laboratory testing. The feasibility level of the results was matched with the Class 1 Water Quality Standard based on the Bali Governor Regulation number 16 of 2016, for the status of the water quality determined by the Pollution Index Method according to the Decree of the State Minister of the Environment No. 115 year 2003. In determining the control strategy, the force field analysis method was applied. The results of the study, there were several parameters that exceeded the quality standard ranging from 18.33-23 mg/l. In addition, the DO content at some points was also not classified as lightly polluted where the highest IP value is Brother with a value of 2.07. The pollution control strategy of Lake Batur produced based on force field analysis was increasing innovative socialization to the community, increasing the promotion of Lake Batur conservation, providing training and entrepreneurial skills as well as providing small-scale business capital assistance, synergizing government policies and programs and forming a pollution control unit.
Keywords: water quality; pollution index; control strategy.
POTENSI PENCEMAR DAN KUALITAS MATA AIR PENIDA DAN GUYANGAN SEBAGAI SUMBER BAKU AIR MINUM DI NUSA PENIDA
Authors: Sudipa Nyoman , Nurjani Ni Putu Suda 
Published: June 2022

Water is an essential requirement for human life. Maintain water availability, both in terms of quality and quantity, in relation to the surrounding environmental conditions. The socio-cultural conditions of the people of Nusa Penida are very much influenced by the presence of water, even social conflicts occur because of the water crisis. The main problem with drinking water raw sources is the availability of water sources which continues to decline from year to year, while water demand continues to increase due to population growth. Areas that have limited water resources will cause economic resources to be used to meet water needs. As an area that has limited water resources, Nusa Penida has springs that are mostly difficult to reach and access by the community. Two springs with quite large discharge are Penida Spring and Guyangan Spring which have been distributed to the community and to fulfill tourism needs. This study aims to determine the feasibility of Penida and Guyangan Springs as a source of drinking water based on Governor of Bali Regulation Number 16 of 2016 concerning Environmental Quality Standards and Standard Criteria for Environmental Damage and to determine the potential sources of pollutants for these two springs. This study uses a quantitative approach combined with primary data sources from direct observations in the field and uses secondary data sources from literature and previous research. The results showed that the Penida and Guyangan Springs were in good condition and met quality standards. Potential sources of pollutants from Penida Springs come from residential activities and tourism activities, while Guyangan Springs does not have pollutants because they are located in a place that is difficult to reach and is in the bowels of Nusa Penida Island.
Keywords: springs, standards, quality, pollutants, Nusa Penida
KUALITAS DAN TINGKAT PENCEMARAN AIR EMBUNG YEH KORI DI DESA JUNGUTAN, KECAMATAN BEBANDEM, KABUPATEN KARANGASEM
Authors: Suryana Wayan , Sudaryati Ni Luh Gede , Sudiartawan I Putu 
Published: June 2022

Many people use surface water for their daily needs. Reservoir water in Yeh Kori Embung is used for various purposes; however, there has not been an inspection of the quality and pollution index that allows contamination of the reservoir. This study aims to evaluate the quality and level of pollution of Yeh Kori Embung Water in Jungutan Village, Karangasem Regency. A descriptive design with a mix-method approach was used in this study. Water samples were taken at five different stations for two periods. Furthermore, physical, chemical and biological examinations are carried out and adjusted to quality standards. The results show that pH (5.2 – 5.4) is in the acidic category and does not meet the requirements while temperature (25.3oC), TSS (32.02 mg/L), DO (6.56 mg/L), BOD ( 1.36 mg/L), COD (10.4 mg/L), NO3-N (4.38 mg/L), Total P (0.021 mg/L), and Fecal Coliform (75.08 MPN/100mL) meet quality requirements. The pollution index (IP=1.085) is classified as lightly polluted. Yeh Kori Embung water does not meet the requirements of class two water quality and requires periodic evaluation and inspection.
Keywords: pH, Pollution level, Reservoir, Water quality, Yeh Kori.
PROFIL BEBERAPA KOMUNITAS PERAIRAN TELUK BANYUWEDANG
Authors: Sara Wijana I Made , Ernawati Ni Made 
Published: June 2022

Banyuwedang Bay is located in the West Bali National Park (TNBB), which is part of the conservation area. There were a lot of tourism activities developed in this area, which potentially caused disruption of the surrounding water conditions. This study aims to determine the profile of various communities in the waters of Banyuwedang Bay and as initial data to see the development of these communities due to the environmental impacts of various activities in the waters and coastal area of Banyuwedang Bay. Data was collected from five observation stations including: Community structure of Plankton, Macrozoobenthos, and seagrass, and coral reef cover. Result shows that the phytoplankton community consist of 9 genera where Nitzschia sp. (Bacillariophyceae) as the dominant species found. Phytoplankton have low diversity index ranging between 0.348 - 1.411. Zooplankton diversity index also has low value 0.693 with three genera found namely Copepoda sp., Nauplius sp. and Daphnia sp. Macrozoobenthos community is composed of 17 macrozoobenthos genera, with low diversity index ranging ??between 0.562-1.809. There are only two species of seagrass, Halophila ovalis and Cymodocea rotundata, which were found in Banyuwedang Bay. Coral reef cover seems to be ranged from poor to good conditions, with 25% - 73% coverage. Based on the result, we concluded that the Banyuwedang Bay has a moderate condition to the growth of plankton and seagrass, but good for coral reefs. Periodic monitoring is needed to find out changes in conditions that can occur in Banyuwedang Bay.
Keywords: Banyuwedang Bay; community structure; macrozoobenthos
Pengaruh Lama Penyimpanan Dengan Pemberian Perasan Jeruk Nipis Terhadap Jumlah Angka Bakteri Pada Daging Ayam Kampung
Authors: Wulansari Nadya Treesna , Padmiswari AA Istri Mas , Antari Ni Wayan Sukma 
Published: June 2022

Penelitian ini bertujuan mengidentifikasi pengaruh lama penyimpanan dengan pemberian perasan jeruk nipis terhadap jumlah angka bakteri daging ayam kampung. Eksperimen dengan model rancangan acak lengkap digunakan dalam penelitian ini. Hasil penelitian menunjukan bahwa adanya pengaruh lama penyimpanan dengan pemberian perasan jeruk nipis terhadap jumlah angka bakteri pada daging ayam kampung. Kandungan asam organik, asam sitrat, minyak atsiri dan limonene yang terkandung dalam jeruk nipis mempengaruhi total bakteri yang terdapat pada daging ayam kampung. Daging ayam kampung dengan pemberian perasan air jeruk nipis yang disimpan pada suhu <40C hingga 12 hari cenderung mengalami peningkatan jumlah cemaran bakteri. Namun, jumlah angka lempeng total bakteri pada daging ayam kampung dibawah dari aturan yang dikeluarkan oleh Badan Standarisasi Nasional Indonesia mengenai batas mutu tingkat cemaran mikroba yaitu 1 x 106 CFU/g. Jumlah bakteri pada daging ayam berdasarkan lama penyimpangan cenderung mengalami peningkatan

Kata kunci: lama penyimpanan, Citrus aurantifolia, bakteri, daging ayam kampung
Formulasi Sediaan Masker Gel Peel-off Ekstrak Daun Kelor (Moringa oleifera Lam.) dan Minyak Atsiri Serai Wangi (Cymbopogon nardus L. Rendle)
Authors: Adhayanti Erlina , Arpiwi Ni Luh , Darsini Ni Nyoman 
Published: June 2022

Masker gel peel-off adalah masker praktis, karena setelah kering masker dapat dilepaskan tanpa dibilas dengan air. Masker gel peel-off berfungsi untuk membersihkan dan mengencangkan kulit wajah. Daun kelor dan minyak atsiri serai wangi dapat digunakan sebagai masker karena memiliki aktivitas antioksidan dan antibakteri yang berperan sebagai anti penuaan serta antijerawat. Tujuan penelitian ini adalah mengetahui kombinasi minyak atsiri serai wangi dan konsentrasi ekstrak daun kelor yang menghasilkan masker terbaik. Minyak atsiri diekstraksi dengan destilasi uap, daun kelor diekstraksi dengan maserasi menggunakan etanol 96%. Masker diformulasi dengan ekstrak daun kelor sebanyak 0, 1, 2, dan 3%. Hasil rendemen minyak atsiri daun serai wangi adalah sebesar 0,36±0,07% b/b. Hasil uji organoleptik menunjukkan bahwa semua formula masker stabil selama masa penyimpanan. Hasil uji sifat fisik masker menunjukkan bahwa formula F3 (ekstrak daun kelor 1% dan minyak atsiri serai wangi 0,30%) menghasilkan formula masker terbaik. Semua formula masker memiliki hasil yang homogen dan memenuhi standar pada uji viskositas, pH dan daya sebar, tetapi formula F1 (kontrol positif) pada uji daya lekat dan formula F1 (kontrol positif) serta formula F2 (kontrol negatif) pada uji waktu mengering tidak memenuhi standar masker. Formula F3 lebih disukai oleh probandus dan semua formula masker tidak menimbulkan iritasi pada kulit.
Kata kunci: Cymbopogon nardus L. Rendle, gel, masker, Moringa oleifera Lam., peel-off
INVESTIGASI BAHAN AKTIF PEGAGAN BERDASARKAN JEJARING DENGAN PROTEIN TARGET : STUDI PENCARIAN OBAT ALZHEIMER SECARA IN SILICO
Authors: Mustakim Moch , Wulandari Vina Fitria , Khoiriyah Nabilah Miftachul , Mawardi Azis , Wulandari Rossa Martha , Fauziyah Thiya Fathiyatul , Mubarakati Nurul Jadid 
Published: June 2022

ABSTRACT
Alzheimer’s disease is a top-10 deadly neurodegenerative disease based on WHO in 2019. Its characterized by a reduction in Choline Acetyltransferase (ChAt) of a substance that acts in the production of asetikolin. One of the ingredients of nature known to play a part in the increased memory of pegagan. To learn about the line of active compound mechanisms used in the treatment of alzheimer's, the study uses an in silico approach by analyzing target proteins and the compound's active compound on a network of compounds. This study used 12 test compounds and the ChAt receptor molecules. Networking design the interactions of these compounds using the sea target and STRING databases. The results of that interactions are visualized on the cytoscape device. Furthermore, the results also suggested thateight of compounds’stest that have interactions with ChAt receptors whereas the target proteins directly linked to ChAt receptors namely Tryptophan 5-hydroxylase 1 (PDB ID: 5tpg) and Zinc finger protein GLI (PDB ID: 4kmd). Thus, the pegagan active compound that acts most closely on protein target is Asiatic Acid, Brahmic Acid, -Humulene, -Caryophyllene, Bicyclogermacrene, Germacrene B, -Pinene, Caryophyllene.
Keywords: Alzheimer's disease, Choline Acetyltransferase (ChAt), pegagan, in silico, network interaction
Carbon Estimation of Cocoa Stands (Theobroma cacao L.) in Agroforestry Area Sumberrejo Village, Pagak, Malang Regency
Authors: Rahayu Rukun , Hakim Luchman , Hayati Ari 
Published: June 2022

INTISARI
Konversi kawasan hutan menjadi perkebunan dapat menimbulkan masalah penurunan kesuburan tanah, erosi, kepunahan flora serta fauna, kekeringan, banjir bahkan perubahan lingkungan global. Masalah ini akan meningkat dari waktu ke waktu sejalan dengan banyaknya luas areal hutan menjadi lahan usaha karena dapat menurunkan serapan emisi karbon melalui fotosintesis. Penerapan konsep agroforestri adalah meningkatkan penyerapan karbon di udara dalam upaya mitigasi perubahan iklim. Tujuan penelitian ini untuk : (1) mengetahui perbedaan karakteristik fisik varietas kakao (Theobroma cacao. L), (2) potensi karbon dan (3) faktor abiotik pada lokasi pengamatan lahan agroforestri. Metode penelitian ini adalah observasi deskriptif untuk megetahui perbedaan karakteristik varietas coklat dengan mengetahui karakter fisik, warna buah, dan warna bijinya, kemudian purposive sampling. untuk mengetahui potensi karbon dilakukan analisis terhadap 120 sampel tegakan pohon coklat pada dua kebun agroforestri yaitu diameter terhadap biomassa dengan rumus allometrik dan pengamatan faktor abiotik menggunakan alat yang sesuai saat dilapang. Hasil observasi biomassa terdapat 3 varietas coklat yaitu criollo sebesar 26 kg/pohon, forastero 28 kg/pohon, dan trinitario 32.4 kg/pohon, serasah rata rata 0,02 kg/m2, distribusi penaung didapat 30 jenis. Faktor abiotik meliputi suhu, 28-29°C, altitude 344-348 m dpl, kecepatan angina 0.3-1.6, intensitas cahaya 766-1008 lux, pH tanah, 6.4-7.2, kelembapan tanah 61-70%, dan curah hujan 200-400 mm dinilai kriteria idealnya berdasarkan literatur.

Kata kunci: konversi, agroforrestri, biomassa, varietas
Potensi Bakteri Sebagai Biodegradasi Lemak Dan Minyak Pada Lingkungan Yang Tercemar Limbah Domestik
Authors: Kawuri Retno , Darmayasa Ida Bagus Gede 
Published: June 2022

Pencemaranan limbah minyak dan lemak ke lingkungan perairan yang berasal dari pengolahan makanan industri, restoran dan dapur atau oleh tumpahan minyak yang tidak disengaja saat ini semakin banyak ditemukan. Biodegradasi menggunakan konsorsium bakteri dapat menjadi salah satu cara yang efektif untuk mengatasi hal tersebut. Tujuan penelitian untuk mengisolasi dan mengidentifikasi strain bakteri dari limbah minyak dan lemak yang mampu melakukan biodegradasi serta potensi dari bakteri pendegradasi dalam mendegradasi minyak dan lemak secara invitro. Sampel diambil dari limbah minyak dan lemak. Pada seluruh sampel dilakukan teknik pengayaan diikuti dengan isolasi strain bakteri untuk menentukan strain mana yang mampu mendegradasi minyak dan lemak secara in vitro. Identifikasi bakteri menggunakan Identifikasi kit BBL Crystal System. Penghitungan total bakteri dilakukan dengan menggunakan metode pengenceran. Hasil menunjukan ditemukan 3 strain bakteri pendegradasi minyak dan lemak yaitu Bacillus licheneformis , Bacillus coagulan dan Psedomonas diminuta. Konsorsium dari ketiga jenis bakteri tersebut mampu mendegradasi limbah minyak dan lemak dengan total bakteri 624x105 CFU/ml hingga 816x105 CFU/ml dengan kontrol tanpa penambahan konsorsium bakteri yaitu sebesar 56x101 CFU/ml. Total bakteri pada berbagai limbah perlakuan dengan konsorsium bakteri berbahan dasar molase pada uji secara invitro menunjukan kemampuan hidup dan berkembang dari konsorsium bakteri yang cukup tinggi yaitu 292x104 CFU/ml hingga 904x104 CFU/ml.. Bakteri yang ditemukan mempunyai kemampuan mendegradasi minyak dan lemak yang bekerja secara sinergistik. Manfaat dari penelitian ini bakteri yang telah ditemukan dapat digunakan sebagai starter pengolahan limbah lemak dan minyak di lingkungan yang tercemar limbah lemak dan minyak domestik.
Kata kunci: konsorsium bakteri, biodegradasi, limbah minyak dan lemak
Perbanyakan Klonal Galur Jeruk Siam Kintamani (Citrus nobilis Lour) Hasil Iradiasi Sinar Gamma
Authors: Lestari Puji Wahyu , Defiani Made Ria , Kosmiatin Mia 
Published: June 2022

Jeruk siam Kintamani disukai oleh konsumen karena memiliki rasa manis, harum, dan daging buahnya lunak. Namun jeruk ini memiliki biji relatif banyak dan warna kulit kurang menarik sehingga kalah bersaing dengan jeruk yang diproduksi oleh negara lain. Alternatif untuk meningkatkan kualitas buah jeruk adalah melalui induksi mutasi in vitro menggunakan sinar gamma. Tunas putative mutan jeruk siam diperbanyak secara in vitro sehingga diperoleh duplikat dari masing-masing mutan. Penggunaan jenis media dan zat pengatur tumbuh pada konsentrasi yang tepat merupakan salah satu kunci keberhasilan dalam kultur jaringan. Tujuan penelitian ini adalah untuk memacu multiplikasi tunas dari eksplan buku. Bahan yang digunakan yaitu plantlet jeruk siam Kintamani hasil iradiasi sinar gamma dengan dosis 0, 4,5, 5, dan 5,5 Gy. Penelitian multiplikasi tunas secara in vitro menggunakan eksplan buku dengan perlakuan 7 jenis media. Hasil penelitian menunjukkan bahwa pada perlakuan media MS VMW + BAP2 + GA2 mampu memperbanyak jumlah tunas, sedangkan media MS VMW mampu meningkatkan jumlah daun dan perbanyakan jumlah akar.
Kata kunci : putative mutan, karakterisasi, multiplikasi, mutasi
Analisis Keanekaragaman, Indeks Nilai Penting dan Index of Cultural Significance Tumbuhan Upacara Ngaben Berdasarkan Tri Mandala di Desa Penglipuran, Bali.
Authors: Wirabumi Ida Bagus Made Bramasta , Kriswiyanti Eniek , Darmadi Anak Agung Ketut 
Published: June 2022

The aim of this research are to know 1)Diversty of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area; 2) Domination Index of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area; 3) Index of Cultural Significance (ICS) Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area. This research is conducted by collecting Ceremonial Plant of Ngaben exist on every quadrat level of the Mandala. The ploting method being used in this research is stratified random sampling with type of plot is single square plot. The data were analyzed using Shanon – Wienner Index, Simpson Index and ICS Turner Index. The result of the research are as follows: 1) Diversty of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area considerably high with score of 2,5302; 2) Score of Domination Index of Ceremonial Plant of Ngaben on Penglipuran Village, Bali based on Tri Mandala Area is also high, which is 0,994214; 3) Higher score of ICS were granted by Kelapa (Cocos nucifera) with score 232; Cabai (Capsicum frustecens) with score of 80; Biu (Musa paradisiaca) with score of 70
Media Environment in Georgia During the 2020 Elections
Authors: Abazov D. , Skvortsova N. 
Published: June 2022

Paper provides the analysis of state-owned and private media companies during parliamentary pre-election race in Georgia in 2020. An attempt to measure the influence of variously interpreted information by different media and how this process, precisely, affects elections was made in this work. It should be noted, the closer the elections are, the more aggressive and radical Georgian media-companies become in their relation to each other and to their audience on a whole.Political polarisation, as consequence of the fierce clash over political power, results in politization of all the social processes, and strongly affects information environment in Georgia. Despite following the democratic blueprint of state formation and announced course for the democratization of political processes, Georgian reality remains far distant from the set goals. Significant role in destabilization of the situation belongs to mass media, whose political activity prevents us from speaking about healthy political environment, nevertheless about flexible state-model in Georgia.