bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics landscape and clinical significance of Siglec family genes: Implications for risk stratification and front line therapies in cutaneous melanoma
Authors: Li Kezhu , Xu Nan 
Published: April 2022

Abstract
Background
Melanoma is widely recognized as the most aggressive and fatal type of skin cancer; however, effective prognostic markers are lacking. The sialic acid-binding immunoglobulin-type lectin (Siglec) gene family plays an important role in the development of tumors and immune escape, but its prognostic role in melanoma remains unknown.
Method
We downloaded simple nucleotide variations, transcriptome profiling, copy number variation data of cutaneous melanoma in TCGA, and transcriptome data of GSE91061 in GEO. 14 Siglecs genes, which were extracted for comprehensive analysis. We typed samples using consistent clustering and constructed risk score using LASSO to predict patient outcomes.
Results
Siglec genes have a high mutation frequency, with up to 8% in SIGLEC7. High expression levels of Siglecs in tumor bulk suggests a better prognosis. Siglecs also show a high degree of synergistic expression. We used unsupervised clustering to create a high expression of Siglec (HES) cluster and a low expression of Siglec (LES) cluster. The HES cluster correlated with high overall survival and increased expression levels of Siglec genes. The HES cluster also showed significant immune cell infiltration and activation of immune signaling pathways. We used least absolute shrinkage and selection operator (LASSO) regression analysis to reduce the dimensionality of Siglec cluster-related genes and constructed a prognostic model composed of SRGN and GBP4, which can risk-stratify patients in both the training and test datasets.
Conclusion
We conducted a multi-omics analysis of the Siglec family genes in melanoma and found that Siglecs play an important role in the occurrence and development of melanoma. Typing constructed using Siglecs can show risk stratification and derived prognostic models can predict a patient's risk score. In summary, Siglec family genes are potential targets for melanoma treatment as well as prognostic markers that can direct individualized treatments and improve overall survival.
In situ formation of MnO 2 /Ni(OH) 2 @nickel foam with porous architecture for triggering persulfate-based advanced oxidation process
Authors: Zhu Fang , Xiao Qixing , Hu Zihao , Ma Jianfeng , Komarneni Sridhar , Mei Yu 
Published: April 2022

Abstract
MnO2/Ni(OH)2 was grown in situ on three-dimensional nickel foam (NF), which was then used to activate potassium persulfate (PS) for orange II (OII) degradation. The structure and morphology of the prepared materials were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). The removal efficiency of OII was 98.68% with MnO2/Ni(OH)2@NF/PS system in 40 min. The three-dimensional hierarchical structure of mesoporous material promoted the efficient and fast electron transfer during catalytic activity. Based on classical quenching experiments and the electron spin resonance (ESR) experiments, ·OH and SO4•− were identified as the active species in MnO2/Ni(OH)2/NF/PS system for OII degradation and a degradation mechanism was proposed for this system. Compared with traditional micro-nano particle catalysts, 3D MnO2/Ni(OH)2@NF has the advantages of convenient recovery and good stability. MnO2/Ni(OH)2@NF catalyst could be recycled for at least three times, and the degradation rate was 89.06% after three cycles. This MnO2/Ni(OH)2@NF is expected to be used as a green heterogeneous catalyst.
Physiological and biochemical changes in Moroccan barley (Hordeum vulgare L.) varieties submitted to drought stress
Authors: Ferioun Mohamed , Srhio Nassira , Bouhraoua Said , Elghachtouli Naima , Louahlia Said 
Published: April 2022

Abstract
Barley (Hordeum vulgare L.) is the second cereal consumed and cultivated by the Moroccan population. However, with climate change, it is predicted that frequent drought periods can cause serious plants growth problems. Thus, the selection of drought tolerant barley varieties becomes highly required to ensure the security of barley’s need. Here, we tested drought tolerance of nine barley Moroccan varieties (ADRAR, AMALOU, AMIRA, FIRDAWS, LAANACEUR, MASSINE, OUSSAMA, TAFFA, and TAMELLALT) based on physiological and biochemical parameters. Drought stress was applied by maintaining filed capacity of pots at 40% (90% for the control), and plants were randomly arranged in greenhouse, under 25°C and natural light conditions. Drought stress reduced relative water content (RWC) and chlorophyll content (SPAD index). It also induced a significant increase of electrolytes leakage, hydrogen peroxide, malondialdehyde (MDA), water soluble carbohydrates, and soluble proteins contents. Higher RWC and proline content levels were recorded in FIRDAWS, LAANACEUR, MASSINE, OUSSAMA and TAFFA, which can be interpreted by high drought tolerance. On the other hand, ADRAR, AMALOU, AMIRA, and TAMELLALT showed higher values of MDA and H2O2 contents, which can be linked with drought sensitivity. Physiological tolerance could be a good criterion for selecting barley drought tolerant varieties.
Discovery of Neurocognitive Phenotypes of Autism By Analyzing Functional Connectivity in the Default Mode Network and Dorsolateral Prefrontal Cortex
Authors: Vasantha Amith 
Published: April 2022

Abstract
Autism spectrum disorder (ASD) is a neurodevelopmental disorder usually presenting as reduced social interaction, lessened verbal communication, and repetitive behavior. Diagnosing ASD is extremely difficult because of its wide variety of symptoms, so it can only be diagnosed through behavioral tests and analysis of developmental history. Resting-state fMRI can help researchers discover a neural substrate for ASD to diagnose it earlier. One prominent fMRI database for ASD research is the Autism Brain Imaging Data Exchange, a large-scale collection of anonymized functional MRI scans subdivided by age, gender, handedness, and scores on behavioral assessments.

This analysis focused on two brain networks: the default mode network (DMN), which is active when minds wander, and the executive network, which is active during the performance of tasks. The medial prefrontal cortex (mPFC), posterior cingulate cortex (PCC), and angular gyrus are nodes of the DMN, and the dorsolateral prefrontal cortex (DLPFC) is the main node in the executive network. Both networks are affected by ASD.

This research used preprocessed resting-state fMRI data to establish neurocognitive phenotypes for ASD. Bivariate correlation was used to compare connectivity in the DMN and DLPFC between ASD and control fMRI scans, and these differences were analyzed for correlations with each patient’s assessment scores. After the Benjamini-Hochberg procedure was applied to reduce the false discovery rate, analysis of these metrics revealed that in ASD patients there was underconnectivity between the right PCC and the right mPFC, while in control patients there was overconnectivity between the right angular gyrus and left DLPFC. ASD is extremely heritable, so phenotypic research is absolutely necessary for discovering more about the genetic causes of ASD, which will speed up ASD diagnosis and help researchers develop more targeted treatments for ASD.
Cyclone “Amphan” evacuation dilemmas of coastal households amidst COVID-19 pandemic: A study of South-Western region of Bangladesh
Authors: Alam Md. Shaharier , Chakraborty Torit , Hossain Md. Zakir , Rahaman Khan Rubayet 
Published: April 2022

Abstract
Cyclone ‘Amphan’ battered the coastal communities in the south-western part of Bangladesh in 2020 during the Covid-19 pandemic. The coastal communities experienced the situation for the first time and were in dilemma whether to stay at home to embrace the cyclone alone or risking the Covid-19 contagion in the cyclone shelters by evacuating themselves. This article intended to explore cyclone Amphan evacuation dynamics among the coastal households amidst COVID-19 pandemic. The study investigated evacuation behaviors among households and explored the impacts of COVID-19 on the evacuation processes. We adopted household survey for collecting primary information and determined 378 samples for interviews at a precision level of 0.05 in fourteen villages. Results demonstrated that despite the utmost effort from the government, 96.6% people in the coastal area received an evacuation order before the landfall, and only 42% people respected the evacuation order. Majority households choose to stay at home because of fear due to Covid-19 in the crowded shelters. Although half of the evacuees were housed in the cyclone shelter, visible COVID-19 protecting facilities were unavailable. Thus, this study would assist future government policies and enhance disaster evacuation plans by incorporating the pandemic to reduce disaster risks in the global south.
Shoulder Pain, Shoulder Disability, and Depression as Serial Mediators Between Stress and Health-related Quality of Life Among Middle-aged Women
Authors: Oh Jihyun , Lee Myung Kyung 
Published: April 2022

Abstract
This study analyzed the mediating effects of shoulder pain, shoulder disability, and depression on the relationship between stress and health-related quality of life among middle-aged women using a serial mediation model. Data on stress, health-related quality of life, shoulder pain, shoulder disability, and depression were collected from women aged 35–64 years living in Seoul, South Korea, from May 13 to 23, 2021, using a self-reported, structured survey. SPSS PROCESS macro (Model 6) and serial mediation analysis were then used to analyze the relationship between stress and health-related quality of life among participants, with shoulder pain, shoulder disability, and depression as mediators. The results indicate that stress had a statistically direct impact on health-related quality of life. In the serial mediation analysis, shoulder pain, shoulder disability, and depression were found to be statistically significant, thus affecting the relationship between stress and health-related quality of life, with an explanatory power of 33%. Therefore, the relationship between stress and health-related quality of life was partially mediated by these variables. Thus, this study suggests the need for healthcare workers to develop methods, such as exercise intervention programs based on various degrees and types of physical activity, to improve health-related quality of life and reduce stress caused by shoulder pain, shoulder disability, and depression among middle-aged women.
Trends and age-period-cohort effect on mortality of cerebrovascular diseases from 2005 to 2019 in urban and rural areas of China
Authors: Zeng Qing , Wang Binbin , Lai Xinyan , Tian Jiangnan , Yang Yulong , Mao Danyi 
Published: April 2022

Abstract
Background:Cerebrovascular diseases are the leading cause of death in China, and the China is undergoing rapid change.However, trends in cerebrovascular disease mortality remains unclear in urban-rural China.Methods:Mortality of cerebrovascular disease (2005-2019) were collected for quinquennial age group stratified by area(urban/rural) from China Statistical Yearbook.The annual changes(APC) and the average annual percent changes(AAPC) of cerebrovascular disease mortality were analyzed by joinpoint regression analysis;age,period,cohort effects on mortality were estimated by age-period-cohort model.Results:Though 2005-2019,standardized mortality significantly rose in rural areas(AAPC=3.3%,95%CI:1%,5.6%) and moderately increased in urban areas(AAPC=1.5%,95%CI:0.6%,2.4%).The age-period-cohort model indicated that there was an obviously age effect and cohort effect..The trend of age effect showed an increase with advancing age.There is a favourable throughout in cohort effects with significantly decrease of both rural and urban areas in China.Conclusions:Both urban areas and rural areas of China,the trend of cerebrovascular disease mortality substantially increased with advance of age.Cerebrovascular disease mortality trend may continue to rise in China.Respectively intervention should be conducted, especially for rural areas.Moreover,paying attention to the physical and mental health of the elderly is also unavoidable.
Rice genotypes significantly affect nitrification inhibition activity in the rhizosphere
Authors: Chen Shending , He Mengqiu , Zhao Chang , Wang Wenjie , Zhu Qinying , Dan Xiaoqian , He Xiaoxiang , Meng Lei , Zhang Shunan , Cai Zucong , Zhang Jinbo , Müller Christoph 
Published: April 2022

Abstract
Biological nitrification inhibitor (BNI) can play an important role in inhibiting nitrification and enhancing nitrogen use efficiency (NUE) in agriculture. However, most of current BNI studies have been conducted under hydroponics. Variations in genotype-mediated inhibition of soil nitrification are still unknown. In this study, two rice genotypes, i.e. Wuyujing 3 (WYJ3) and Wuyunjing 7 (WYJ7), which were reported to have weak and strong BNI capacity under hydroponics, respectively, and four soils with different pH (i.e. JX (pH 5.09), FJ (pH 6.00), SC1 (pH 7.96) and SC2 (pH 7.94)) were selected. N uptake rates (esp. NH4+ uptake, UNH4), soil N transformation rates and NUE were quantified by 15N tracing experiment to assess the effects of rice genotypes on nitrification inhibition activity. Results showed that rice genotypes with high BNI exudation (i.e. WYJ7) had lower autotrophic nitrification rate (ONH4) and higher UNH4 in rhizosphere compared to WYJ3. ONH4 in WYJ7 reduced by 0.05, 0.42, 1.14, and 0.48 mg N kg− 1 d− 1 compared with WYJ3 for JX, FJ, SC1 and SC2, respectively. Abundance of AOB in soils planted WYJ7 was lower than WYJ3, which was the key factor affecting ONH4. NUE of WYJ7 was higher than WYJ3, although difference between genotypes was not significant. NUE was negatively correlated with ONH4 (P < 0.05). Our results indicated that some rice genotypes can optimize their N acquisition by regulating soil N transformations (particularly nitrification). Developing rice genotype with strong BNI exudation capacity could be a suitable management practice to increase NUE and yield.
The relationship between Job Stress and Work-Related Quality of Life among Emergency Medical Technicians: A cross-sectional Study
Authors: Shima Hashemi , Mohammed Merzah , Mehdi Rezaei , Peyman Astaraki , Mehdi Birjandi , Firoozeh Ghazanfari 
Published: April 2022

Abstract
Background and objectives: This study was conducted to determine the relationship between job stress and WRQoL among emergency medical technicians (EMTs) in Lorestan Province, Western Iran. Methods: This is a cross-sectional study using census method among 430 emergency medical technicians who had been engaged in their respective units for more than six months from all emergency facilities in Lorestan Province. Data were collected from April to July 2019 using two standard questionnaires: Job Stress (HSE) and Work-Related Quality of Life (WRQoL). The odd ratio with 95% Confidence Interval (CI) was used to declare the statistical association (p ≤ 0.05).Results: All participants were exclusively males, with a mean age of 32±6.87 years. The overall average score of job stress using the HSE scale was 2.69±0.43; while the overall quality of working life score was 2.48±1.01.The type of working shift was found to have a significant impact on the HSE-average score, (F (3,417) = 5.26, P = 0.01); and on the WRQoL-average score, (F (3,417) = 6.89, P< 0.01).Conclusion: Two-thirds of EMTs working in governmental hospitals had job stress and a low quality of work-related life. Work shift was statistically significant associated with EMTs’ job stress and WRQoL. To improve the WRQoL among EMTs, we suggest the urgent need for organizational intermissions that aim to diminish work-related stress. Furthermore, rescheduling should be explored as a strategy for reducing stress caused by shift work.
GIS-Based Asset Mapping, Utilities Infrastructure Mapping, Consumer Mapping&amp; Studying Feasibility Parameters for Water Supply System
Authors: Dhumal shantanu , Sharma kalpeshkumar , Tambde Ashish , Chikane Sakshi , Dhage Sakshi 
Published: April 2022

Abstract
To use the application of geographical information system for data collection for augmentation and feasibility study of the existing water supply system. The use of GIS (geographical information system) in this project is to prepare a schematic map and identify water demand, which is done by several relevant parameters. Mapping of the MIDC area can be done by geo sensing or by capturing drone images. Then these images are transferred to arc GIS Where the digitization of images is done and attribute tables are filled with data such as consumers’ water demand, consumers’ water meter readings. As the available drawings and data were around 30-year-old, all the drawings are prepared on AutoCAD with the latest information. Along with satellite images and drone images of the MIDC area DGPS (Differential global positioning system) survey is also done to generate topographical data. Application\improvements: this study concludes what parameters are required for a feasibility study of the water supply system and application of GIS for carrying out the project and its application in the mapping of utilities of industrial areas in MIDC. GIS is used over another method because of its high accuracy and its capability to show all the utilities which also include underground utilities, structures, and any other such assets in the real world. With all these advantages GIS has an edge over any other method of surveying and data collection which then allows theda decisions decide on planning and execution also with the data collected augmentation can be done if required.
A Novel Lambert W Representation of PSP MOS Model for Circuit Simulators
Authors: afifi ahmed Abdel Monem 
Published: April 2022

Abstract
A new Lambert w representation for the surface potential from the PSP MOS model point of view is proposed. The resultant of this methodology is to benefit from merging of PSP model accuracy as a compact model and iterative simplicity of the Lambert W function. This increases the speed and accuracy of the circuit simulators specially for both cases of weak as well as moderate inversion. The suggested approach leans on reformatting the implicit equation of the surface potential in the form of Lambert W representation via a proper mathematical curve fitting. The fitting parameters has been tolerated for wide temperature range of about 350C for each range. This is done through making use of the dominant dependency of the normalized body effect coefficient. The proposed algorithm is validated for a typical 0.18um technology by means of MATLAB 2016 and proves an accuracy of ± 5% to the original PSP model for a temperature range of [-18 o C, 100 o C].
Functional and pasting properties of cassava starch in Ethiopian varieties: Potentiality of industrial utilization
Authors: Raya Shimeles Shumi , Kume Desta Geta , Kuffi Kumsa D. 
Published: April 2022

Abstract
Starch extracted from different sources and varieties show various functional, pasting, and physicochemical properties which affect its industrial applications. In view of, this research work was conducted to study functional and pasting properties of starches extracted from Qulle (Q) and Kello (K) cassava varieties to assess their potential industrial utilization. The starch was extracted by a standard wet extraction method. The proximate analysis showed that (K) starch has a moisture content (11.4%), ash content (1.1%), crude fat content (0.11%), protein content (0.52%), crude fiber content (0.01%) and total carbohydrate (86.85%). Whereas (Q) starch possesses moisture content (10.6%), ash content (0.13%), crude fat content (0.13%), protein content (0.35%), crude fiber content (0.09%) and total carbohydrate (88.7%). Except fat and crude fiber contents, the rest proximate parameters showed significance different between Q and K’s starch at p < 0.05. Furthermore, the amylose content of each variety of starch were calculated and found to be 26.29% (Q) while, 19.16% (K) with a significance different among them at p < 0.05. These variations directly affect the water solubility, swelling power and water absorption properties of the starch. Moreover, the pasting properties were examined and observed that the peak and breakdown viscosities were found to be high for (K) starch due to low amylose content. Indicating that the relatively high final viscosity and peak temperature nature of (Q’s) starch affect its end use applications. The functional and pasting parameters of starch showed that there is a significance different between Q and K’s starch properties at p < 0.05. Overall, the functional and pasting properties of casava varieties’ (Q and K) starch influenced by the amylose content and proximate composition which potentially impact the industrial utilization.
Mechanical acupuncture stimulation of HT7 attenuates alcohol intake in alcohol self- administration rats via the medial prefrontal cortex-habenula-ventral tegmental area pathway
Authors: Seo SU Yeon , Bang Se Kyun , Kang Suk-Yun , Cho Seong Jin , Choi Kwang-Ho , Ryu Yeonhee 
Published: April 2022

Abstract
Shenmen (HT7) acupuncture point stimulation is effective in alleviating alcoholism. Our study confirmed the effect of HT7 stimulation on reducing alcohol consumption via the up- and downregulation of habenula (Hb) microglial activity. To investigate the role of the Hb in mediating acupuncture effects, we evaluated the role of Hb, medial prefrontal cortex (mPFC) and ventral tegmental area (VTA) after acupuncture stimulation in the rat brain after alcohol intake. The number of active lever presses in alcohol (10% v/v) intake rats was significantly reduced following HT7 stimulation. HT7 stimulation not only attenuated the increased microglial activity and Sigma1 receptor level in the Hb and BDNF in the mPFC but also decreased TH in the VTA. Phospho-TrkB inhibition via ANA-12 microinjection into the mPFC reduced Hb microglial activity, decreased VTA TH expression and reduced alcohol intake. Blocking the Sigma1 receptor via BD1047 microinjection and inhibiting microglia via minocycline microinjection into the Hb reduced VTA TH expression and reduced alcohol intake. Controlling mPFC and Hb activity with acupuncture consequently regulated VTA TH neurons, reducing alcohol consumption. These results indicate that acupuncture treatment can alleviate alcohol consumption via the mPFC-Hb-VTA signaling pathway and could be a candidate for alcoholism therapy.