bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Lifestyle Modifications and Endometrial Proteome Changes of Women With Polycystic Ovary Syndrome and Obesity
Authors: Abdulkhalikova D. , Sustarsic A. , Vrtačnik Bokal Eda , Jancar N. , Jensterle M. , Burnik Papler T. 
Published: June 2022

Polycystic ovary syndrome (PCOS) is a polyendocrine disorder and the most common endocrinopathy in women of reproductive age. Affected women have an elevated prevalence of being overweight and obese. Our study sought to determine how weight loss associated with lifestyle changes affects the endometrium specific proteome, endocrine-metabolic characteristics, and motor capabilities of obese women with PCOS and infertility. A group of 12 infertile women under the age of 38 with PCOS and BMI ≥30 kg/m2were included in the study. An evaluation was performed by a gynecologist and an endocrinologist. The weight-loss program lasted 8 weeks under the guidance of a professional trainer. Endometrial sampling during a period of implantation window for proteome determination was performed before and after weight loss. In endometrial samples at the end of the study increased protein abundance was recorded for Legumain, Insulin-like growth factor-binding protein 7, Hepatocyte growth factor receptor, Keratin, type II cytoskeletal 7, and Cystatin-B, while the B-lymphocyte antigen CD20 protein abundance decreased. Our results also indicate significantly lowered fasting blood glucose level and free testosterone concentration and significant improvements in body composition and physical capacity. This study may open up the venues for investigating important biomarkers that may affect endometrial receptivity.Clinical Trial Registrationhttps://clinicaltrials.gov/ct2/show/NCT04989244?term=NCT04989244&amp;draw=2&amp;rank=1, identifier: NCT04989244.
Specific miRNAs Change After 3 Months of GH treatment and Contribute to Explain the Growth Response After 12 Months
Authors: Catellani Cecilia , Ravegnini Gloria , Sartori Chiara , Righi Beatrice , Lazzeroni Pietro , Bonvicini Laura , Poluzzi Silvia , Cirillo Francesca , Predieri Barbara , Iughetti Lorenzo , Giorgi Rossi Paolo , Angelini Sabrina , Street Maria Elisabeth 
Published: June 2022

ContextThere is growing evidence of the role of epigenetic regulation of growth, and miRNAs potentially play a role.ObjectiveThe aim of this study is to identify changes in circulating miRNAs following GH treatment in subjects with isolated idiopathic GH deficiency (IIGHD) after the first 3 months of treatment, and verify whether these early changes can predict growth response.Design and MethodsThe expression profiles of 384 miRNAs were analyzed in serum in 10 prepubertal patients with IIGHD (5 M, 5 F) at two time points before starting GH treatment (t−3, t0), and at 3 months on treatment (t+3). MiRNAs with a fold change (FC) &gt;+1.5 or &lt;-1.5 at t+3 were considered as differentially expressed. In silico analysis of target genes and pathways led to a validation step on 8 miRNAs in 25 patients. Clinical and biochemical parameters were collected at baseline, and at 6 and 12 months. Simple linear regression analysis and multiple stepwise linear regression models were used to explain the growth response.ResultsSixteen miRNAs were upregulated and 2 were downregulated at t+3 months. MiR-199a-5p (p = 0.020), miR-335-5p (p = 0.001), and miR-494-3p (p = 0.026) were confirmed to be upregulated at t+3. Changes were independent of GH peak values at testing, and levels stabilized after 12 months. The predicted growth response at 12 months was considerably improved compared with models using the common clinical and biochemical parameters.ConclusionsMiR-199a-5p, miR-335-5p, and miR-494-3p changed after 3 months of GH treatment and likely reflected both the degree of GH deficiency and the sensitivity to treatment. Furthermore, they were of considerable importance to predict growth response.
Chemical Analysis and Electrochemical Monitoring of Extremely Low-Concentration Corrosive Impurity MgOHCl in Molten MgCl2–KCl–NaCl
Authors: Gong Qing , Ding Wenjin , Chai Yan , Bonk Alexander , Steinbrecher Julian , Bauer Thomas 
Published: June 2022

MgCl2–KCl–NaCl is a promising thermal energy storage (TES) material and heat transfer fluid (HTF) with high operating temperatures of &gt;700°C for next-generation concentrating solar power (CSP) plants. One major challenge for future implementation of the molten chloride TES/HTF technology arises from the presence of some corrosive impurities, especially MgOHCl, a hydrolysis product of hydrated MgCl2. Even extremely low-concentration MgOHCl (tens of ppm O in weight) can cause unneglectable corrosion of commercial Fe–Cr–Ni alloys, which limits their service time as the structural materials in the molten chloride TES/HTF system. Thus, the chemical analysis and monitoring techniques of MgOHCl at the tens of ppm O level are vital for corrosion control. In this work, a chemical analysis technique based on direct titration and a high-precision automatic titrator was developed for an exact measurement of MgOHCl at the tens of ppm O level. It shows a standard deviation below 5 ppm O and an average error below 7 ppm O when the concentration of MgOHCl is 36 ppm O. Moreover, compared to other methods available in some literature reports, it can exclude the influence of co-existing MgO on the MgOHCl concentration measurement. This chemical analysis technique was used to calibrate the previously developed electrochemical method based on cyclic voltammetry (CV) to achieve reliable in situ monitoring of MgOHCl in the MgCl2–KCl–NaCl molten salt at a concentration as low as the tens of ppm O level. The in situ monitoring technique shows a monitoring limitation of &lt;39 ppm O. The two techniques for MgOHCl measurement developed in this work could be used to develop an in situ corrosion control system to ensure the long service time of the molten chloride TES/HTF system in next-generation CSP plants.
Predicting the Effects of Climate Change on the Suitable Habitat of Japanese Spanish Mackerel (Scomberomorus niphonius) Based on the Species Distribution Model
Authors: Yang Tianyan , Liu Xingyu , Han Zhiqiang 
Published: June 2022

Japanese Spanish mackerel (Scomberomorus niphonius) is an important commercial fish species with a seasonal migratory habit in China. At present, no study has reported the seasonal variation of its potential suitable habitat. Therefore, we used biomod2 ensemble model to predict the potential suitable habitat of S. niphonius in different seasons through its current distribution sites and future environmental variables. Our results showed that the ensemble model had a high accuracy, with true skill statistics and receiver operator characteristic values of 0.91 and 0.98, respectively. Under the Representative Concentration Pathway (RCP) 4.5 scenario, the reduction rate of suitable habitats in summer of 2050 will be 33.2%, and those for the rest of the seasons will reach 3.5%. The reduction rate in summer of 2100 will be 43.5%, and those for the remaining seasons will reach 6.5%. In addition, this reduction rate will become more serious with the increase in emission scenario and time, especially in the RCP8.5 in which the summer reduction rate is as high as 88.9%. Habitat changes in summer show a strong temperature sensitivity. The distribution centroid of S. niphonius will shift to higher latitudes under RCP 4.5 and RCP 8.5. Depth and temperature are the two most important factors that affect the distribution of this species. The results can provide basic data for understanding the adaptability of S. niphonius to climate change and establishing an elastic ecosystem.
Heterotopic Pregnancy Secondary to in vitro Fertilization-Embryo Transfer: Risk Factors and Pregnancy Outcomes
Authors: Zhu Shaomi , Fan Yiyue , Lan Ling , Deng Tianqing , Zhang Qinxiu 
Published: June 2022

BackgroundWith the widespread application of assisted reproduction technology (ART) and increased pelvic inflammatory disease, the incidence of heterotopic pregnancy (HP) has risen. However, the risk factors and treatment of HP remain indefinite.ObjectivesTo explore risk factors affecting the incidence of HP secondary to in vitro fertilization-embryo transfer (IVF-ET) and pregnancy outcomes after surgical treatment of HP.Methods29 patients with HP and 116 with an intrauterine-only pregnancy (IUP) after IVF-ET during the same period were included retrospectively from January 2015 to September 2020.ResultsPatients with HP had a higher proportion of previous ectopic pregnancies, multiple abortion history (≧2 times) and tubal indication for IVF than IUP. Besides, they had a greater possibility to end in spontaneous abortion (31.03 vs.13.79%, P = 0.028) and preterm delivery (25.00 vs. 7.00%, P = 0.035), less possibility to result in a live birth (58.62 vs. 78.45%, P = 0.028). History of multiple abortions (≥2 times) [odds ratio (OR) 3.031, 95% confidence intervals (CI) 1.087–8.453; P = 0.034], tubal infertility (OR 3.844, 95% CI 1.268–11.656; P = 0.017), previous ectopic pregnancies (OR 2.303, 95% CI 0.625–8.490; P = 0.021) and number of embryo transfer (OR 0.300, 95% CI 0.092–0.983; P = 0.037) resulted in an elevated proportion of HP in IVF treatment. Shorter operative duration, smaller size of the ectopic mass and location in the ampulla of the fallopian tube were associated with higher chance of survival in the coexistent intrauterine pregnancy after surgical treatment.ConclusionsPrevious history of ectopic pregnancy, multiple abortions, tubal infertility and multiple-embryo transfer may be considered as meaningful risk factors of subsequent HP following IVF-ET. In patients with HP treated by surgery, shorter operative duration, smaller size of the ectopic mass and location in the ampulla of the fallopian tube means better reproductive prognosis.
The Evaluation of Physiological Index Changes and Safety Work of Female Medical Staff With Different Medical Protection Standards in the Ward of COVID-19
Authors: Zhao Min , Zhao Jianhui , Yan Junbing , Gao Xiaoye 
Published: June 2022

BackgroundEffective personal protective equipment (PPE) contribute to the prevention of COVID-19 infection. However, it is necessary to evaluate the potential risk of different medical protections in the isolation ward of COVID-19.ObjectivesWe aimed to explore the dynamics in physiological indexes of medical staff under primary and secondary PPE in the isolation ward of COVID-19 and provide the scientific basis for determining the safe work strategy.Materials and MethodsIn this study, 30 female nurses were selected to simulate medical work under the primary or secondary PPE, respectively. The oral temperature, axillary temperature, heart rate, respiratory rate, blood oxygen saturation, and blood pressure were measured and recorded every 20 min. The subjective adverse symptoms were recorded every 30 min. The blood glucose and weight of the individuals were measured and recorded before and after the trial.ResultsThe results indicated that the median trial persistence time in the participants with moderate-intensity work wearing the secondary PPE (70.0 min) was much lower than that with moderate-intensity work wearing the primary PPE (180 min) and with light-intensity work wearing the primary PPE (110 min; p &lt; 0.05). Importantly, the heart rate, oral/axillary temperature, and respiratory rate of physiological indexes of the participants under moderate-intensity work wearing the secondary PPE increased significantly faster than the primary PPE (p &lt; 0.001), while blood oxygen saturation decreased significantly faster than the primary PPE (p &lt; 0.001). In addition, the proportions of subjective adverse symptoms (such as dry mouth, dizziness, palpitations, and anhelation) were much higher than primary PPE (p &lt; 0.001). The average sweat volume and blood glucose consumption of participants under moderate-intensity work wearing primary PPE were higher than secondary PPE (p &lt; 0.001).ConclusionThe combination of an exacerbated workload and secondary PPE worn by COVID-19 healthcare workers increases the change in physiological indicators, and in some cases the adverse symptoms, which can affect and even suspend their medical work. For any medical institution, there is room for improvement in terms of bioethics of a “Job Well Done” to reduce the risks of medical activities under secondary PPE.
Healthy Organs Uptake on Baseline 18F-FDG PET/CT as an Alternative to Total Metabolic Tumor Volume to Predict Event-Free Survival in Classical Hodgkin's Lymphoma
Authors: Morland David , Triumbari Elizabeth Katherine Anna , Maiolo Elena , Cuccaro Annarosa , Treglia Giorgio , Hohaus Stefan , Annunziata Salvatore 
Published: June 2022

PurposeHealthy organs uptake, including cerebellar and liver SUVs have been reported to be inversely correlated to total metabolic tumor volume (TMTV), a controversial predictor of event-free survival (EFS) in classical Hodgkin's Lymphoma (cHL). The objective of this study was to estimate TMTV by using healthy organs SUV measurements and assess the performance of this new index (UF, Uptake Formula) to predict EFS in cHL.MethodsPatients with cHL were retrospectively included. SUV values and TMTV derived from baseline 18F-FDG PET/CT were harmonized using ComBat algorithm across PET/CT systems. UF was estimated using ANOVA analysis. Optimal thresholds of TMTV and UF were calculated and tested using Cox models.Results163 patients were included. Optimal UF model of TMTV included age, lymphoma maximum SUVmax, hepatic SUVmean and cerebellar SUVmax (R2 14.0% - p &lt; 0.001). UF &gt; 236.8 was a significant predictor of EFS (HR: 2.458 [1.201–5.030], p = 0.01) and was not significantly different from TMTV &gt; 271.0 (HR: 2.761 [1.183–5.140], p = 0.001). UF &gt; 236.8 remained significant in a bivariate model including IPS score (p = 0.02) and determined two populations with different EFS (63.7 vs. 84.9%, p = 0.01).ConclusionThe Uptake Formula, a new index including healthy organ SUV values, shows similar performance to TMTV in predicting EFS in Hodgkin's Lymphoma. Validation cohorts will be needed to confirm this new prognostic parameter.
The Influence of 24-h Ambulatory Blood Pressure on Cognitive Function and Neuropathological Biomarker in Patients With Alzheimer's Disease
Authors: Li Lixia , Wang Weijia , Lian Tenghong , Guo Peng , He Mingyue , Zhang Weijiao , Li Jinghui , Guan Huiying , Luo Dongmei , Zhang Weijia , Zhang Wei 
Published: June 2022

PurposeThis study aimed to investigate the influence of 24-h ambulatory blood pressure (BP) on cognitive function and neuropathological biomarkers in patients with Alzheimer's disease (AD) at the stages of mild cognitive impairment (MCI) and dementia.MethodsThe patients with AD were divided into the MCI (AD-MCI) group and the dementia (AD-D) group. Notably, 24-h BP variables, including BP level, coefficient of variation (CV) of BP, and pulse pressure, were collected and compared between the two groups. The correlations between 24-h BP variables and the scores of cognitive domains were analyzed. The independent influencing factors of cognitive domains of patients with AD were investigated. The levels of neuropathological biomarkers of AD, including β amyloid (Aβ)1−42, phosphorylated tau (P-tau), and total tau (T-tau), in cerebrospinal fluid (CSF) were measured and compared between the two groups, and the correlations between 24-h BP variables and the levels of neuropathological biomarkers of AD were analyzed.ResultsDaytime CV of systolic BP (SBP) was significantly increased in the AD-D group compared to that in the AD-MCI group. The 24-h and daytime CV of SBP and ambulatory pulse pressure were significantly and negatively correlated with memory score. The average 24-h and average daytime SBP level and CV of SBP, daytime CV of diastolic BP (DBP), and 24-h, daytime, and night-time ambulatory pulse pressure were significantly and negatively correlated with language score. The average 24-h SBP level, daytime CV of SBP, and 24-h, daytime, and night-time ambulatory pulse pressure were significantly and negatively correlated with attention score. Further analysis indicated that daytime CV of SBP as well as age and course of disease were the independent influencing factors of language. Age was also the independent influencing factor of memory and attention of patients with AD. T-tau level in CSF in the AD-D group was significantly higher than that in the AD-MCI group, but the levels of Aβ1−42, P-tau, and T-tau in CSF were not correlated with 24-h ambulatory BP variables.ConclusionDaytime CV of SBP was the independent influencing factor of language in patients with AD. The AD-D patients had significantly severe neurodegeneration than AD-MCI patients, which was, however, not through the influence of 24-h ambulatory BP variables on neuropathological biomarkers of AD.
Correlation Between Pre-Operative Sleep Disturbance and Post-Operative Pain in Patients With Rotator Cuff Tear
Authors: Wu Hui , Su Wanying , Huang Shengtao , Xiao Yili , Lu Liang 
Published: June 2022

ObjectivesThis study aims to investigate the relationship between preoperative sleep disturbance and postoperative pain in patients with a rotator cuff tear, and to provide a theoretical basis for taking corresponding interventions to alleviate postoperative pain in patients with rotator cuff tear.MethodsA total of 87 patients, who had undergone shoulder arthroscopy due to rotator cuff injury in Hunan Provincial People‘s Hospital from January to October 2021, were selected as the research subjects. The Pittsburgh Sleep Quality Index (PSQI) was used to evaluate the sleep quality of patients with rotator cuff tears. All patients were divided into the low sleep quality group (PSQI score &gt;7 points, n = 61) and the high sleep quality group (PSQI score ≤7 points, n = 26). Postoperative pain was assessed by using the Numerical Rating Scale (NRS). General clinical data of the patients were collected 1 day, 2 days, and 1 month after surgery. Univariate and multivariate analyses of influencing factors were performed in patients with moderate or above pain at 1 month after surgery.ResultsThe score of postoperative pain of patients in the high sleep quality group was significantly lower than that of patients in the low sleep quality group (P &lt; 0.05). A total of 35 patients (40.2%) had moderate or above pain 1 month after surgery, including 5 patients (19.2%) in the high sleep quality group and 30 patients (49.2%) in the low sleep quality group. The incidence rate of the low sleep quality group was significantly higher than that of the high sleep quality group (P = 0.009). After controlling confounding factors, preoperative sleep disturbance of patients was still independently associated with the occurrence of moderate or above pain 1 month after surgery (OR = 3.794, 95% CI: 1.261–11.409, P = 0.018).ConclusionPreoperative sleep disturbance can increase the risk of postoperatively moderate or above pain threshold in patients with rotator cuff tear. Paying more attention to and actively improving preoperative sleep disturbance can effectively promote postoperative pain management in patients with rotator cuff tears.
Oncological and Reproductive Outcomes in Patients With Advanced-Stage Ovarian Immature Teratoma: Experience From a Tertiary Center
Authors: Wang Dan , Cang Wei , Zhu Shan , Jia Congwei , Cao Dongyan , Yang Jiaxin , Xiang Yang 
Published: June 2022

ObjectiveTo evaluate the oncological and reproductive outcomes in patients with advanced-stage ovarian immature teratoma (IMT).MethodsWe retrospectively reviewed the medical records of patients with advanced-stage IMT who were treated with surgery between January 1985 and December 2020. Fertility-sparing surgery (FSS) was defined as preservation of the uterus and at least one adnexa. Oncological outcomes were compared between patients who underwent FSS and radical surgery. Patients who underwent FSS were also contacted to gather information about their menstrual history and reproductive outcomes.ResultsForty-six patients fulfilled the inclusion criteria, of whom 38 underwent FSS and eight were treated with radical surgery. Fifteen patients suffered recurrence after a median follow-up time of 74.2 months (range: 4.1–434.1 months). The 5-year disease-free survival (DFS) and overall survival (OS) rates were 69.1% and 89.9%, respectively. Multivariate analysis identified suboptimal cytoreductive surgery as the only independent risk factor for recurrence. There was no significant difference in DFS or OS between patients with different surgical procedures. Ten of the 15 relapsed patients had optimal salvage surgery and all remained alive with no evidence disease. Among the 32 patients who underwent FSS, 29 resumed menstruation after surgery, and five of seven patients who designed pregnancy achieved a total of five successful pregnancies.ConclusionsOvarian IMT has a favorable prognosis, even when diagnosed at an advanced stage. FSS is feasible in patients with advanced-stage IMT who wish to preserve their fertility. Patients may benefit from optimal cytoreductive surgery during initial and salvage surgery.
Predicting Chemo-Radiotherapy Sensitivity With Concordant Survival Benefit in Non-Small Cell Lung Cancer via Computed Tomography Derived Radiomic Features
Authors: Liu Yixin , Qi Haitao , Wang Chunni , Deng Jiaxing , Tan Yilong , Lin Lin , Cui Zhirou , Li Jin , Qi Lishuang 
Published: June 2022

BackgroundTo identify a computed tomography (CT) derived radiomic signature for the options of concurrent chemo-radiotherapy (CCR) in patients with non-small cell lung cancer (NSCLC).MethodsA total of 226 patients with NSCLC receiving CCR were enrolled from public dataset, and allocated to discovery and validation sets based on patient identification number. Using CT images of 153 patients in the discovery dataset, we pre-selected a list of radiomic features significantly associated with 5-year survival rate and adopted the least absolute shrinkage and selection operator regression to establish a predictive radiomic signature for CCR treatment. We performed transcriptomic analyzes of the signature, and evaluated its association with molecular lesions and immune landscapes in a dataset with matched CT images and transcriptome data. Furthermore, we identified CCR resistant genes positively correlated with resistant scores of radiomic signature and screened essential resistant genes for NSCLC using genome-scale CRIPSR data. Finally, we combined DrugBank and Genomics of Drug Sensitivity in Cancer databases to excavate candidate therapeutic agents for patients with CCR resistance, and validated them using the Connectivity Map dataset.ResultsThe radiomic signature consisting of nine features was established, and then validated in the dataset of 73 patients receiving CCR log-rank P = 0.0005, which could distinguish patients into resistance and sensitivity groups, respectively, with significantly different 5-year survival rate. Furthermore, the novel proposed radiomic nomogram significantly improved the predictive performance (concordance indexes) of clinicopathological factors. Transcriptomic analyzes linked our signature with important tumor biological processes (e.g. glycolysis/glucoseogenesis, ribosome). Then, we identified 36 essential resistant genes, and constructed a gene-agent network including 10 essential resistant genes and 35 candidate therapeutic agents, and excavated AT-7519 as the therapeutic agent for patients with CCR resistance. The therapeutic efficacy of AT-7519 was validated that significantly more resistant genes were down-regulated induced by AT-7519, and the degree gradually increased with the enhanced doses.ConclusionsThis study illustrated that radiomic signature could non-invasively predict therapeutic efficacy of patients with NSCLC receiving CCR, and indicated that patients with CCR resistance might benefit from AT-7519 or CCR treatment combined with AT-7519.