bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of neutrophil extracellular traps in early microthrombosis and brain injury after subarachnoid hemorrhage in mice
Authors: Hao Xiaoke , Zeng Zongwei , Liang Liang , Feng Zhou , Li Wu , Xiong Binyuan , Guo Peiwen , Zhang Qiang , Chen Yujie , Feng Hua , Chen Zhi 
Published: April 2022

Abstract
Microthrombosis plays an important role in secondary brain injury after experimental subarachnoid hemorrhage (SAH), but the specific mechanism of microthrombosis remains unclear. The purpose of this study was to investigate the role of neutrophil extracellular traps (NETs) in microthrombosis after SAH. SAH was induced in male C57BL/6 mice using an endovascular perforation technique. The marker protein of NETs, citrullinated histone H3 (CitH3), was significantly elevated in the cerebral cortex after SAH, and was co-labeled with microthrombi. Both depletion of neutrophils by anti-Ly6G antibody and DNase I treatment significantly reduced the formation of NETs and microthrombi, ameliorated neurological deficits, brain edema, BBB disruption, and neuronal injury at 24 h after SAH induction. Cerebral hypoperfusion in the first hours after SAH is a major determinant of poor neurological outcome, in this study, we found that DNase I treatment significantly improved the restoration of early cortical perfusion after SAH. In addition, DNase I treatment also significantly attenuated cerebrospinal fluid (CFS) flow and glymphatic system dysfunction after SAH, which was associated with the diffusion barrier caused by microthrombi in the paravascular space after SAH. In conclusion, NETs is associated with early microthrombosis after SAH, it may be a novel therapeutic target for early brain injury (EBI) after SAH.
Endoscopic therapy strategy for upper gastrointestinal early cancer complicated with esophagogastric varices
Authors: Deng Chao , Wu Suhua , Xu Feng , Lv Lin , Li Juan , Chen Zhiji , Zhou Zhihang , He Song 
Published: April 2022

Abstract
Background: Endoscopic ligation and histoacryl injection have become the main methods for esophagogastric varices (EGV) in cirrhosis patients. And endoscopic submucosal dissection (ESD) has been widely used in the treatment of early gastrointestinal cancer. The present study aims to discuss the endoscopic therapy strategy for upper gastrointestinal early cancer in cirrhotic patients companied with EGV.Methods: This study retrospectively analyzed the clinical data and endoscopic treatment characteristics of 12 patients with cirrhosis complicated with both EGV and early tumor of the upper digestive tract.Results: The therapeutic decision depends on the relative location of early cancer to EGV and the general condition of patients. The international normalized ratio (INR) should be corrected to be no more than 1.5 and the platelet count no less than 50×106/ml. The early cancers were dissected if the lesions were far from the EGV. On the other hand, the EGV should be treated first if the early cancers were close to the varices. En bloc resection was 100% (11/11) for all ESD procedures. The average operation time was 55 min (25-180 min). No significant complications were observed during the perioperative period. During an average follow-up time of 22.2 months (16-28 months), neither local recurrence nor enlarged lymph nodes was found. The remaining one patient lost the ESD chance due to the cancer progression after transjugular intrahepatic portosystemic shunt (TIPS) treatment.Conclusions: ESD is a safe and effective way to remove early gastrointestinal cancer in patients complicated with EGV after comprehensive evaluation.
Global warming and tropical cyclones in the Arabian Sea and the Gulf of Oman
Authors: Pourkerman Majid , Marriner Nick , Hamzeh Mohammadali , Mohammadpor Gholamreza , Lahijani Hamid , Tavakoli Morteza , Morhange Christophe , Amjadi Sedigheh , Lak Razyeh 
Published: April 2022

Abstract
An increase in the frequency and intensity of tropical cyclones (TCs) under global warming is global concern. To probe this relationship, regions of where TCs are strong but rare are particularly important. Within this context, the Arabian Sea (AS) and the Gulf of Oman (GO) constitute excellent case-study examples. TCs in the AS and GO are rare phenomena but during the last two decades three strong events (Gonu, Phet and Shaheen) have occurred, leading to a total of 6.07 billion USD in damages and 159 fatalities. In this study we use historical TC records, satellite data, atmospheric and oceanographic models to probe the link between TCs and global warming. The results demonstrate that global warming has modified three important environmental parameters in the AS and GO: 1- there has been an increase in water temperature and density in the Persian Gulf (PG), 2- there has been a northward migration of the monsoon belt and an increase in upwelling on the Oman coast, 3- there has been an increase in heat advection and transfer by cyclonic/anticyclonic Mesoscale Eddy (ME) and geostrophic currents that are involved TC intensification. These processes have led to a shallow warm and high-salinity water plume from the PG to the AS, and an accumulation of the ME center between 16°N and 22°N. Warm geostrophic currents lead the TCs to the Mesoscale Eddy centers, favoring an increase in TC intensity at higher latitudes. As a result, strong TCs have made landfall on the Gulf of Oman and the Arabian Sea coasts. Our study suggests that the frequency of strong TCs will increase due to the effects of global warming. Education programs via international platforms such as the International Ocean Institute (IOI) and UNESCO are required for the countries at risk.
Aging-related changes in strength and cross-sectional area of knee flexor muscles
Authors: Park Soo Yeon , Yoon Kyoung Ho , Ko Taeg Su , Lee Hee Sung 
Published: April 2022

Abstract
Background: We aimed to study the association between flexor muscle strength in the knee joint and cross-sectional area (CSA) on magnetic resonance imaging (MRI). We also evaluated aging-related changes in flexor muscle strength and CSA. We hypothesized that muscle strength is associated with muscle CSA on MRI and that such relationships would differ among the flexor muscles. Furthermore, we hypothesized that muscle strength and CSA would decline, and the reduced rate of muscle CSA would differ among the flexor muscles with aging.Methods: A total of 252 patients (mean age, 33.6 years; range, 11–66 years) who visited the outpatient clinic with knee pain between September 2006 and August 2009 were retrospectively analyzed. The CSA of each knee joint flexor muscle (biceps femoris, sartorius, gracilis, semitendinosus, and semimembranosus) was measured on MRI axial images at the supra-patellar level. We evaluated flexor muscle strength (peak torque, N.m) using a Cybex dynamometer at 60 and 180°/s and evaluated its correlation with CSA.Results: Mean CSA was 605.4 mm2 for the semimembranosus, 444.7 mm2 for the biceps femoris, 282 mm2 for the sartorius, 55.4 mm2 for the semitendinosus, and 34.1 mm2 for the gracilis. Mean peak torque was 67.4 N.m and 52.7 N.m at 60°/s and 180°/s, respectively. CSA was positively correlated with flexion strengths of 60°/s (r = 0.363, p = 0.000) and 180°/s (r = 0.354, p = 0.000). Muscle strength was associated with CSA for all muscles, except the gracilis (r = 0.056, p = 0.375). Flexion strength decreased significantly with age after the third decade of life. Total CSA decreased with age (r = −0.247, p = 0.000). CSA of the biceps femoris, sartorius, semimembranosus, and semitendinosus decreased significantly, while CSA of the gracilis tended to decrease non-significantly with aging.Conclusions: Muscle strength was associated with total muscle CSA on MRI and the CSA of every muscle but the gracilis. Flexion strength decreased significantly with aging after the third decade of life. Total CSA decreased significantly with aging. CSA of the biceps femoris, sartorius, semimembranosus, and semitendinosus decreased significantly, whereas that of the gracilis decreased slightly with aging.Level of Evidence: Level III, retrospective comparative study
New approach for the formulation of High Performance Concrete Valorisation of local materials
Authors: Messaoud Sabah Ben 
Published: April 2022

Abstract
High performance concrete (HPC) is an innovative concrete that finds its place in modern buildings. The new high-performance concrete formulation and conception techniques have improved sustainability and acquiring remarkable mechanical properties compared to conventional concretes. The objective of this work is to make a contribution to understanding the influence of factors such as the W/ C ratio and the granular class on the mechanical and physical properties of high performance concretes. In the formulations of HPC aggregates by their high mass and volume proportion, play an important role, the Sherbrook and Dreux-Gorisse method has been used. When selecting aggregates it is necessary to know their intrinsic properties. These properties influence the performance of concrete in particular the quality of the granulate cimentary adhesion. At the end of our work, the examination of the results obtained made it possible to establish the correlations between the formulations studied and the physico-mechanical characteristics of the concrete compositions (HPC 25, HPC 16, HPC 8). The results of this study show that the use of three granular classifications (DMAX8, DMAX16 and DMAX25) and three Report W / C (0.25, 0.30, and 0.35) in two different conservation environment (EP drinking water and sea water ), clearly proves that the composition of high performance concrete is HPC25 concrete with an W/ C = 0.25 ratio has reached the largest mechanical strength of 80 MPa for different environments of conservation. When selecting aggregates it is necessary to know their intrinsic properties, these properties influence the performance of concrete. In general, there is a slight decrease in the compressive resistance of the specimens stored in seawater, it can be said that the conservation life has not had effect on the resistance (28 days). The effect of aggressive environment can appear in the long term.
Imine Reversal Mediates Charge Separation and CO2 Photoreduction in Covalent Organic Frameworks
Authors: Streater Daniel , Wang Denan , Fiankor Christian , Kennehan Eric , Liu Daohua , Kohlstedt Kevin , Luo Long , Zhang Jian , Huang Jier 
Published: April 2022

Abstract
We investigate the correlation between CO2 reduction performance in twin COFs with reversed imine connectivity and reveal that imine-linkage imparts intramolecular charge transfer (ICT) directionality – an effect we term an “ICT Tesla Valve.” This reversal also affects the coordination of Re molecular catalyst (MC) and leads to a slowdown in light driven CO2→CO photoreduction. ICT towards Re MC in Re-f-COF facilitates efficient CO2 photoreduction while performance is reduced when ICT is direct away from Re MC in Re-r-COF. Combining theory with experimental data shows that ICT directionality affects the charge separation mechanism which is particularly evident in the much longer-lived excited state of Re-f-COF compared to Re-r-COF. These findings are unprecedented, revealing not only that the linker chemistry should be recognized as a key factor to consider in the development of COF photocatalysts but also provides a unique approach to tailor photocatalysts by controlling the direction of ICT.
The Stat3 inhibitor F0648-0027 is a potential therapeutic against rheumatoid arthritis
Authors: Kaneko Yosuke , Ozawa Shin-ichiro , Sato Yuiko , Kobayashi Tami , Matsumoto Tatsuaki , Miyamoto Kana , Kobayashi Shu , Harato Kengo , Hirono Shuichi , Matsumoto Morio , Nakamura Masaya , Niki Yasuo , Miyamoto Takeshi 
Published: April 2022

Abstract
BackgroundRheumatoid arthritis (RA) is a disease characterized by chronic joint inflammation, pain and joint destruction, leading to alteration in activities of daily living, yet pathological mechanisms underlying the condition are not fully clarified. To date, various therapeutic agents have been developed as RA therapy including DMARDs and/or biological agents that target inflammatory cytokines or inhibit JAK. Here we asked whether inhibiting signal transducer and activator of transcription 3 (Stat3) activity would antagonize RA. MethodsStat3 forms dimers when activated and undergoes nuclear translocalization; thus we screened approximately 4.9 million small compounds as potential blockers of protein-protein interactions required for Stat3 dimerization using in silico screening. We then tested candidate Stat3-inhibiting activity in vitro by analyzing expression of IL-6, a Stat3 target, in compound-treated fibroblasts. We also analyzed expression of RANKL, a cytokine essential for osteoclastogenesis, in vitro. We then evaluated anti-arthritis effects of candidate compounds in vivo in collagen-induced arthritis model mice. Effects of the candidate compounds on inhibiting Stat3 phosphorylation and nuclear localization following IL-6 stimulation of fibroblasts were analyzed by an immune histocochemical analysis as well as western blotting.ResultsWe identified 15 as strong candidates as potential blockers of protein-protein interactions required for Stat3 dimerization using in silico screening from approximately 4.9 million small compounds. Four of the 15 significantly inhibited IL-6 as well as RANKL expression induced by IL-6. One compound, F0648-0027, significantly inhibited arthritis development without apparent adverse effects. F0648-0027 also significantly blocked Stat3 phosphorylation and nuclear localization following IL-6 stimulation of fibroblasts. ConclusionsThese data suggest that Stat3 is a target for RA, and that F0648-0027 could serve as a therapeutic reagent against the condition.
Effective Diagnostic Biomarkers, Immune Infiltration, miRNA and N6-methyladenosine regulators in Lupus Nephritis
Authors: Peng Zhishen , Lin Xiaobing , Liang Xiaofeng , Lin Zien , Lin Weiyi , Wei Shanshan 
Published: April 2022

Abstract
Objective
Lupus nephritis (LN) is the most common complication of systemic lupus erythematosus (SLE), which is an autoimmune disease involving multiple organs. This study aimed to explore the potential molecular mechanism of LN by bioinformatic analysis.
Method
In total, 130 LN, 25 living healthy donors were included to explore the differentially expressed genes (DEGs). The protein-protein interaction (PPI) network of DEGs were developed using the STRING database. Additionally, five algorithms were used to find the hub genes and their diagnostic effectiveness was predicted using receiver operator characteristic curve (ROC) analysis. CIBERSORT was used to evaluate the infiltration of immune cells in LN. Furthermore, the mRNA-miRNA network was constructed. Finally, we explored the landscape of N6-methyladenosine (m6A) regulators in LN.
Result
Two hub genes—FOS and IGF1—were found by the intersection of five different algorithms. FOS and IGF1 could jointly diagnose LN with the most excellent specificity and sensitivity. LN patients had lower activated and resting dendritic cells (DCs) while higher M1 macrophages and activated NK cells than HC. FOS had a positive correlation with activated mast cells and a negative correlation with resting mast cells. IGF1 had a positive correlation with activated DCs and a negative correlation with monocytes. MiR-155 is considered as the hub miRNA. And m6A modification is related to the severity of renal injury and involved in the pathogenesis of LN. MiRNA may affect the occurrence and development of LN by targeting m6A regulators.
Conclusion
Activated DCs, resting DCs, M1 macrophages, and activated NK cells may play a role in LN pathogenesis. FOS, IGF1 and miR-155 may be new potential molecular markers for the pathogenesis, progression and new molecular targets for treatment of LN. MiRNA may affect the occurrence and development of LN by targeting m6A regulators.
Isotopic Tracing of Phosphorus Fraction Distribution in Chinese Fir Seedlings with High Phosphorus Efficiency Using 32P Labelling
Authors: Zou Xianhua , Liu Qingqing , Huang Zhijun , Chen Sitong , Wu Pengfei , Ma Xiangqing 
Published: April 2022

Abstract
Purpose
The ability of plants to redistribute and transfer phosphorus (P) fractions determines their adaptability to P limitation. However, the mechanisms of P utilization and transport remain unknown in Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) from the perspective of P fraction distribution.
Methods
In this study, we investigated the distribution and translocation patterns of total P and different P fractions in the M1 Chinese fir genotype, which has high P-use efficiency (PUE), using 32P tracking that can accurately trace the migration pathways of exogenous P after plant absorption.
Results
We found that total P in roots was higher than in stems or leaves under P limitation when the amount of exogenous P absorbed by M1 reduced significantly. Under low-P, plants optimized P allocation, which led to higher PUE than under high-P, with the highest PUE in leaves, followed by stems and roots. The M1 genotype maintained a high ratio of soluble P (i.e., inorganic-P and ester-P) in its leaves and stems that increase P mobility and recycling under P limitation. In roots, P content shifted from soluble inorganic-P and ester-P to insoluble P (i.e., nucleic-P), but total P concentration was relatively stable, which may ensure root growth and exogenous P absorption under P limitation.
Conclusion
Our results confirm the high PUE of the M1 genotype, which reduces P demand, maintains aboveground productivity, and optimizes the allocation of P among P fractions in response to P-limitation.
Prevalence and anatomical characteristics of bifid and trifid mandibular canals: a computed tomography analysis
Authors: Ramaglia Luca , Blasi Andrea , Galasso Luigi , Pezzella Vitolante , Cuozzo Alessandro , Iorio-Siciliano Vincenzo 
Published: April 2022

Abstract
Purpose: To assess the prevalence and configuration of bifid (BMC) and trifid (TMC) mandibular canals using computed tomography, describing the anatomical characteristics of the accessory canals, especially of the retromolar type.Methods: Computed tomographic scans of 123 patients were analyzed. BMCs were identified and the patterns of bifurcation were classified, including trifid canals. The width of accessory canals was measured. Retromolar canals were further classified according to their course and morphology, while their position and width were evaluated using linear measurements on CT/CBCT images.Results: The majority part of patients (53.6%) presented at least one BMC or TMC. A percentage of 36.2% of mandibular canals were bifid, while 4.5% trifid. The forward canals (12.6%) and retromolar canals (10.2%) were the most common among BMCs. In relation to the retromolar canals, 60% were vertical and 40% curved, with a mean width of 1.03 ± 0.28mm.Conclusion: BMCs and TMCs are common 3D radiographic findings, so that they should be considered as anatomical variants, not anomalies. Preoperative CT or CBCT evaluation should aid to identify these variations, analyzing the position and course.
Laser wakefield acceleration of electrons using high-order Bessel-Gauss beams for driver beam and electron bunch guiding
Authors: Tomkus Vidmantas , Girdauskas Valdas , Abedi-Varaki Mehdi , Račiukaitis Gediminas 
Published: April 2022

Abstract
High-intensity laser pulse propagating through a gas target disturbs uniform plasma distribution. Plasma density structures, created by high-order Bessel-Gauss beams for guiding the Gaussian beam driver and laser wakefield acceleration of electrons, are analysed using Wake-T and Fourier-Bessel Particle-In-Cell (FBPIC) simulation tool. The utilisation of Bessel-Gauss beams increases the acceleration distance and energy of accelerated electrons up to 50% relative to the Gaussian beam of the same intensity.
Leveraging university-industry partnerships to optimise postgraduate nursing education
Authors: Theobald Karen A , Fox Robyn , Burridge Christine , Thomson Bernadette , Fox Amanda 
Published: April 2022

Abstract
Background: Industry and higher education sectors devote considerable, but independent resources to deliver postgraduate nursing education. This leads to duplication, uncertainty among students, and critical gaps in nursing education. Establishing and sustaining meaningful partnerships between invested university and industry stakeholders can strengthen workforce capability and improve patient care. Methods: To evaluate the feasibility and effectiveness of using a University-Industry Integration Framework to develop a postgraduate nursing education program. Prospective mixed methods cohort study (STROBE). A co-design approach, using an established University-Industry Integration Framework, leveraged expert stakeholder partnerships to contextualise knowledge and service need for developing a postgraduate education program for cancer care nurses. Results: All participants (n=46) were 100% satisfied with the online resources, support, and communication processes applied. Qualitative data generated three major analytical interpretations (reciprocity, flexible adaptations, authentic learning), highlighting the experiences and connections and how the partnership evolved. Program participants (n=15) undertook a six-week cancer education program with eight responding to the survey with overwhelming satisfaction (100%), increasing their knowledge and skills. While barriers were evident, three quarters (n=6) indicated these were addressed and enabled progress in the program. However, 63% (n=5) were not satisfied with the program workload. Conclusions: University and industry partners can apply the University-Industry Integration Framework and deliver a successful postgraduate education program for cancer care services. Within a co-design partnership it is possible to develop strategies and processes to overcome barriers and deliver a program for mutual benefit. The culmination of this successful education program has enhanced collaborations between partners and likely will sustain the offering of future co-design endeavours.
Treatment with camrelizumab plus tyrosine kinase inhibitors with or without TACE for intermediate-advanced hepatocellular carcinoma: A clinical efficacy and safety study
Authors: Yin Yaolin , Li Yonghe , Xiong Yongfu , Xian Yin , Meng Chun , He Yi , He Kun , Li Jingdong 
Published: April 2022

Abstract
Purpose: This research compared the curative effect and safety of camrelizumab plus tyrosine kinase inhibitors (TKIs) combined with transcatheter arterial chemoembolization (TACE) to those of camrelizumab plus TKIs for the treatment of intermediate-advanced hepatocellular carcinoma (HCC).Methods: From January 2019 to July 2021, 47 patients with intermediate-advanced HCC were included in this retrospective study. After screening, 44 eligible patients were split into two arms: the camrelizumab + TKI + TACE arm (n=28) and the camrelizumab + TKI arm (n=16). The primary endpoints were overall survival (OS) and progression-free survival (PFS), while tumor response and adverse events (AEs) served as secondary endpoints.Results: The median OS was 12.6 months for the 44 patients. The median PFS (P=0.0248, 7.2 months vs. 3 months), objective response rate (ORR) (21.43% vs. 6.25%) and disease control rate (DCR) (57.14% vs. 18.75%) were better in the camrelizumab + TKI + TACE arm than in the camrelizumab + TKI arm. After correcting for the effects of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin (TBil), the treatment effect of the camrelizumab + TKI + TACE arm (HR=0.274, 95% CI=0.096-0.784, P=0.016) was still superior. The common AEs in the two groups included reactive cutaneous capillary hyperplasia (n=17), myelosuppression (n=19) and liver dysfunction (n=12). However, AEs of grade ≥ 3 were equal between the groups.Conclusion: Camrelizumab + TKI + TACE therapy was more effective than camrelizumab + TKI therapy for intermediate-advanced HCC, and toxicity was manageable.