bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preliminary Results Regarding the Genetic History of a Native Horse Breed- Genetic Relationship between Sire Stallions and Broodmares
Authors: Maftei M. , Ianitchi D. , Raducuta I. , Nistor L. , Dronca D. , Ahmadi M. 
Published: July 2022

Background: This study is a part of an ample research concerning the genetic history of Hucul horse breed. The genetic analysis studies are part of animal genetic resources Management because just start of them we elaborate the strategies for inbreeding management. The itself genetic relationship of a population is the average genetic relationship of all individuals between them, existing at a time. Therefore, this parameter can be described three components: the genetic relationship between stallions, between mother mares themselves and the genetic relationship between stallions and mares. The last component is most important because of the average population inbreeding increases in successive generations. Methods: The research was carried out during 2012-2020. The material are represented by genealogical information from reproductive nucleus of Hucul breed from Lucina studfarm. The sample extracted from population was represented by seven sire stallions and seventy-three broodmares. The method are based on A matrix coefficients. All analysis were performed in the profile lboratories of Animal Sciences Faculties from Bucharest and Timisoara. Result: Between individual average genetic relationship values of stallions with mares, is found significant differences, highlighted by the global test of homogeneity test (F = 4.891). These differences allow us to assert that, through stallions selection, is made a reproductive discrimination much more intense than in mares case. The average genetic relationship of the population was calculated at 0.1941±0.0053.
Choose Wisely: Surgical Selection of Candidates for Outpatient Anterior Cervical Surgery Based on Early Complications Among Inpatients
Authors: Carlson Bayard C. , Dawson John M. , Beauchamp Eduardo C. , Mehbod Amir A. , Mueller Benjamin , Alcala Christopher , Mullaney Kevin J. , Perra Joseph H. , Pinto Manuel R. , Schwender James D. , Shafa Eiman , Transfeldt Ensor E. , Garvey Timothy A. 
Published: July 2022

Background:
Anterior cervical discectomy and fusion (ACDF) and cervical disc arthroplasty (CDA) are attractive targets for transition to the outpatient setting. We assessed the prevalence of rapid responses and major complications in the inpatient setting following 1 or 2-level ACDFs and CDAs. We evaluated factors that may place patients at greater risk for a rapid response or a postoperative complication.

Methods:
This was an institutional review board-approved, retrospective cohort study of adults undergoing 1 or 2-level ACDF or CDA at 1 hospital over a 2-year period (2018 and 2019). Data on patient demographic characteristics, surgical procedures, and comorbidities were collected. Rapid response events were identified by hospital floor staff and involved acute changes in a patient’s clinical condition. Complications were events that were life-threatening, required an intervention, or led to delayed hospital discharge.

Results:
In this study, 1,040 patients were included: 888 underwent ACDF and 152 underwent CDA. Thirty-six patients (3.5%) experienced a rapid response event; 22% occurred >24 hours after extubation. Patients having a rapid response event had a significantly higher risk of developing a complication (risk ratio, 10; p < 0.01) and had a significantly longer hospital stay. Twenty-four patients (2.3%) experienced acute complications; 71% occurred >6 hours after extubation. Patients with a complication were older and more likely to be current or former smokers, have chronic obstructive pulmonary disease, have asthma, and have an American Society of Anesthesiologists (ASA) score of >2. The length of the surgical procedure was significantly longer in patients who developed a complication. All patients who developed dysphagia had a surgical procedure involving C4-C5 or more cephalad. Patients with a rapid response event or complication were more commonly undergoing revision surgical procedures.

Conclusions:
Rapid response and complications are uncommon following 1 or 2-level ACDFs or CDAs but portend a longer hospital stay and increased morbidity. Revision surgical procedures place patients at higher risk for rapid responses and complications. Additionally, older patients, patients with chronic obstructive pulmonary disease or asthma, patients who are current or former smokers, and patients who have an ASA score of ≥3 are at increased risk for postoperative complications.

Level of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
FERN-ARTHROPOD INTERACTIONS FROM THE MODERN UPLAND SOUTHEAST ATLANTIC RAINFOREST REVEALS ARTHROPOD DAMAGE INSIGHTS TO FOSSIL PLANT-INSECT INTERACTIONS
Authors: CENCI ROMULO , HORODYSKI RODRIGO SCALISE 
Published: July 2022

ABSTRACTFerns were among the first broadleaved plants in the fossil record. We assessed fern-arthropod interactions in modern ferns (Monilophyta) as a model for comparison with damage on ferns in the fossil record. We found that the functional feeding groups of margin feeding, hole feeding, surface feeding, piercing and sucking, oviposition, mining, and galling was present on 13 species of ferns at elevations ranging from 750 to 900 meters along mountain slopes of the Brazilian Atlantic Rainforest. We recognized 17 damage types (DTs), including one new DT, and provide implications for interpreting damage on leaves in the fossil record. Nine fossil DTs with modern analogues were found on ferns. Evaluation of damage on modern ferns demonstrate that the variation in the abundance and damage signatures of external feeding, piercing and sucking, galling, and mining enhances understanding of damage patterns on fossil leaves. The taphonomic implications to fossil plant-insect interactions are provided based on the sampling of modern arthropod damage and the preservation biases on ferns. Arthropod remains that are poorly attached to ferns, such as silk webs, eggs, and spider egg sacs, as well as insect exuviae, pupae, and body parts, might provide an important feature for arthropod preservation associated with fern leaves in the plant fossil record. This integrative method demonstrates that DT signatures on modern ferns indicate that ferns are an important host plant for herbivorous arthropods. Consequently, the same importance of ferns as host plants of arthropod herbivores likely was present in deep time.
Evaluating exercise limitation, gas exchange inefficiency and recurrence risk in PE patients by cardiopulmonary exercise testing
Authors: Zhu Hanqing , Zhu Wei , Sun Xingxing , Cao Yuan , Xu Jianhua , Pudasaini Bigyan , Yang Wenlan , Guo Jian 
Published: July 2022

Abstract
Background
Pulmonary embolism (PE) which is a form of venous thromboembolism (VTE) can induce acute symptoms and post-PE syndrome. Cardiopulmonary exercise testing (CPET) can evaluate exercise responses of multiple systems, including respiratory, cardiovascular, hematopoietic and skeletal muscle systems.
Methods
We retrospectively collected 103 subjects’ data of demographics, hematologics, CT pulmonary angiogram (CTPA), pulmonary function test (PFT) and CPET at Shanghai Pulmonary Hospital from March 2015 to October 2021. The 103 subjects were composed of 10 healthy people as control, 25 patients who had been diagnosed with PE and 68 patients who were diagnosed with PE. The CPET performance differences of the three groups of subjects and subgroups of PE patients divided according to CTPA were analyzed. Spearman correlation test was performed to analyze the correlation between CPET and Blood test parameters of the enrolled subjects. Univariate logistic regression analysis, multivariable cox regression analysis and Kaplan–Meier cumulative survival analysis was used to find CPET parameters for predicting the risk of PE recurrence.
Results
The exercise tolerance (load @ peak), metabolic capacity (VO2 @ AT, VO2 @ Peak), ventilatory efficiency (VE/VCO2 @ AT, VE/VCO2 @ Peak, OUEP, LOWEST VE/VCO2), gas exchange (PETCO2 @ AT, PETCO2 @ Peak, PETO2 @ AT), and cardiovascular response (VO2/HR) of healthy people, Post-PE and PE subjects were different. The ventilatory efficiency (VE/VO2, VE/VCO2, LOWEST VE/VCO2, OUPE, VE/VCO2 slope), gas exchange (PETO2, PETCO2) of PE subgroups divided according to CTPA were also different. VO2 @ AT (% pred) can be used to predict the risk of PE recurrence.
Conclusion
CPET is an effective and responsible method for the assessment of pulmonary embolism.
STOP? II trial: Cluster randomized clinical trial to test the implementation of a toolbox for structured communication in the operating room: Study protocol
Authors: Keller Sandra , Tschan Franziska , Semmer Norbert , Trelle Sven , Manser Tanja , Beldi Guido 
Published: July 2022

Abstract
BackgroundSurgical care, which is performed by intensely interacting multidisciplinary teams of surgeons, anesthetists and nurses remains associated with significant morbidity and mortality. Intraoperative communication has been shown to be associated with surgical outcomes, but tools ensuring efficient intraoperative communication are lacking.In a previous study, we developed the StOP?-protocol that fosters structured intraoperative communication. Before critical phases of the operation, the responsible surgeon initiates and leads one or several StOP?s. During a StOP?, the surgeon informs about the progress of the operation (Status), next steps and proximal goals (Objectives), possible problems (Problems), and encourages all team members to voice their observations and ask questions (?). In a before-after study performed mainly in visceral surgery, we found effects of the StOP?-protocol on mortality, length of hospital stay and reoperation. We intend to assess the impact of the StOP?-protocol in a cluster randomized trial, in a wider variety of surgical specialties (i.e. general, visceral, thoracic, vascular surgery, surgical urology, and gynecology).The primary hypothesis is that the consistent use of the StOP?-protocol by the main surgeon reduces patient mortality within 30 days after the operation. The secondary hypothesis is that the consistent use of the StOP?-protocol by the main surgeon reduces unplanned reoperations, length of hospital stay, and unplanned hospital readmissions.Methods: This study is designed as a multicenter, cluster-randomized parallel group trial. Board-certified surgeons of participating clinical departments will be randomized 1:1 to the StOP? intervention group or to the standard of care (control) group. The intervention group will undergo a training to use the StOP?-protocol and receive regular feedback on their compliance with the protocol. The surgeons in the control group will communicate as usual during their operations. The unit of observation will be operations performed by cluster surgeons. Consecutive patients will be enrolled over four months per cluster. A total of 400 surgeons will be recruited, and we expect to collect patient outcome data for 14’000 surgical procedures.Discussion: The StOP?-protocol was designed as a tool to structure communication during surgical procedures. Testing its effects on patient outcomes will contribute to implement evidenced-based interventions to reduce surgical complications.Trial registration: ClinicalTrials.gov; NCT05356962
Ergonomic Design and Modeling of Reconfigurable Wheelchair for Medical Purpose
Authors: Paytyan Tatevik Ashot , Melikyan Artashes Arsen , Zirakyan Srbuhi , Stepanyan Hovsep 
Published: July 2022

Abstract
As the design of various industrial items for the disabled is associated with a number of complications, requires a great deal of medical and constructive training, all the necessary standards have been studied. These standards relate to the ergonomic requirements of disabled people, the details of problems, as well as the technologies and opportunities needed in industry. The main types of existing wheelchairs are presented. Their advantages and disadvantages have been studied. Attention was also paid to the existing market in the given sphere, taking into account the possible market competition as a result of the project implementation. Due to the constructive, ergonomic-aesthetic study of the devices, a conceptual design of an innovative product in the given field was developed. The study and usage of all this gives an opportunity to make wheelchairs designed for the disabled, accessible to a wider range of people, as well as ergonomically comfortable, technologically innovative and competitive in the commercial market.
Clinical features and differences of Miller Fisher syndrome in southern China: Retrospective analysis of 72 patients from 13 provinces in southern China
Authors: Yu Hang , Ding Man , Chu Hong , Zhou Rumeng , Yao Jiajia , Fu Rong , Liu Yue , Xiao Zheman , Lu Zuneng 
Published: July 2022

Abstract
Objectives: We aimed to analyze the clinical features of Miller Fisher syndrome (MFS) in southern China and compare it features presenting in other countries. Methods: Medical records of patients with a diagnosis of Guillain-Barré syndrome (GBS) admitted to 31 tertiary hospitals, located in 13 provinces in southern China, from 1 January 2013 to 30 September 2016, were collected and retrospectively reviewed. Results: A total of 72 patients (36 from rural area and 36 from urban area) were enrolled. Their age at onset was 47.42±15.65 years old. Winter (29.2%) and spring (41.7%) were the seasons with the peak incidence of MFS. Thirty-eight (52.8%) patients had a history of upper respiratory tract infection one week before onset, while 4 (5.6%) patients experienced gastrointestinal infections. Among the 65 patients who underwent lumbar puncture, 48 (73.8%) patients exhibited albumincytologic dissociation in the cerebrospinal fluid study. Overall, 83.5% patients presented with limited outward movement of the eye, 64.2% had bilateral symmetrical ophthalmoplegia, and only 25.4% had unilateral ophthalmoplegia. No significant differences were observed between the rural and urban areas, such as age of onset (p=0.777) or sex distribution (p=0.609). Patients with complete ophthalmoplegia were older than those with incomplete ophthalmoplegia ( 64.53±7.69 years vs 43.19±14.40 years, p<0.001). Conclusion: Winter and spring are the peaks of MFS incidence. The incidence is higher in men than in women. The most common pattern of ophthalmoplegia in MFS is bilateral symmetrical ophthalmoplegia, There is no urban-rural difference in MFS in southern China. Patients with complete ophthalmoplegia were older than incomplete ophthalmoplegia. There is no significant difference of MFS between southern China and other East Asian countries.
Correlation of a guidewire maximum insertion length with tortuous radial artery and the success rate during transradial coronary angiography
Authors: Li Hongsong , Chen Xia , Sha Shuang , Xu Xiangdong , Chen Yingmin 
Published: July 2022

Abstract
Background
Percutaneous coronary intervention (PCI) is a safe and effective therapy for patients with obstructive coronary artery disease (CAD). We aimed to assess the correlation between the success rate of angiography and the maximum insertion length and resistance of a soft-tipped guidewire.
Methods
521 patients were treated by successful radial artery puncture. According to whether the guidewire resistance, the patients were divided to three groups. 17 patients were maximum insertion length of guidewire ≤ 30 cm when resistance was encountered (group 1). 17 patients were maximum insertion length of guidewire between 30 cm and 45 cm when resistance was encountered (group 2). 487 patients were no resistance encountered (group 3).
Results
The coronary angiography success rates of group 1, 2, and 3 were 52.94%, 47.05%, 98.97%, respectively. Typically, angiography can be completed in patients with Ω-shaped, S-shape or Z-shaped tortuosity.
Conclusions
The maximum insertion length of straight guidewire and resistance can be used to determine radial artery status. The radial artery tortuosity or spasm significantly affects the success rate of coronary angiography.
Slope stability analysis method of unsaturated soil slopes considering pore gas pressure caused by rainfall infiltration
Authors: Tian Wenjing , Peiffer Herman , Malengier Benny , Xue Song , Chen Zhongtian 
Published: July 2022

Abstract
The variation of pore gas pressure caused by rainfall infiltration is an important factor that leads to slope failures. The purpose of this study is to propose a new slope stability analysis method that considers pore gas pressure and examines the effect of airflow on slope stability by using a numerical method. A water-air two-phase flow analysis was conducted to investigate the distribution of pore air pressure, pore water pressure as well as water saturation triggered by rainfall infiltration. Then the variation of the load resulting from pore gas pressure was incorporated into the slope stability analysis method on the basis of the unsaturated soil shear strength theory and the residual thrust method to simulate the influence of airflow on the Tanjiahe landslide in China. In order to study the infiltration behavior with respect to initial saturation, water and gas flow analyses were performed considering various initial statuses of saturation under similar settings. Results showed that the pore gas pressure between the slope surface and slip band clearly varied and that it decreased during the process from the slide bed to the deep direction. Then the pore water pressure formed in the saturated zone was transferred by the airflow to the slope toe. As a result, because the pore gas pressure gradient increased the thrust of the slide mass, the safety factor decreased over time. Moreover, in the first step, the magnitude of infiltration decreased with an increase in initial saturation, while when the magnitude dropped to the minimum value, then it went up with an increase in the initial saturation. The maximum value was usually reached at a saturation degree of 0% or 100%. When evaluating slope stability, the safety factor obtained by the slope stability analysis method that considered the water-gas coupling effect was much lower than when it was not considered during the process of a similar seepage. Such impact on the slope failure was significant and may provide a practical reference for a hazard assessment to control rainfall-induced landslides.
Plasma level of ATPase inhibitory factor 1 (IF1) is associated with type 2 diabetes risk in humans: a prospective cohort study
Authors: Silva Julie Pires , Wargny Matthieu , Raffin Jeremy , Croyal Mikaël , Duparc Thibaut , Combes Guillaume , Genoux Annelise , Perret Bertrand , Vellas Bruno , Guyonnet Sophie , Thalamas Claire , Langin Dominique , Moro Cédric , Viguerie Nathalie , Rolland Yves , Barreto Philipe Souto , Cariou Bertrand , Martinez Laurent O. 
Published: July 2022

Abstract
Aim: Mitochondrial dysfunction is associated with the development of type 2 diabetes (T2D). It is thus of clinical relevance to identify plasma biomarkers of mitochondrial dysfunction associated with the risk of T2D.ATPase inhibitory factor 1 (IF1) endogenously inhibits mitochondrial ATP synthase activity. Here, we analyzed association of the plasma IF1 level with markers of glucose homeostasis and with the conversion to new-onset diabetes (NOD) in individuals with prediabetes.Methods: In the IT-DIAB prospective study, the baseline plasma level of IF1 was measured in 307 participants with prediabetes. The primary outcome was the incidence of NOD within five years of follow-up. Cross-sectional analysis of the IF1 level was also done in two independent interventional studies. Correlations between plasma IF1 and metabolic parameters at baseline were assessed by Spearman’s correlation coefficients, and the association with the risk of NOD was determined using Cox proportional‐hazards models.Results: In IT-DIAB, the mean IF1 plasma level was lower in participants who developed NOD than in those who did not (537 ± 248 versus 621 ± 313 ng/mL, p=0.01). The plasma IF1 level negatively correlated with clinical variables associated with obesity and insulin resistance, including the BMI (r = -0.20, p=0.0005) and HOMA-IR. (r = -0.37, p<0.0001). Conversely, IF1 was positively associated with plasma markers of cardiometabolic health, such as HDL-C (r = 0.63, p<0.0001) and apoA-I (r = 0.33, p<0.0001). These correlations were confirmed in cross-sectional analyses. In IT-DIAB, the IF1 level was significantly associated with a lower risk of T2D after adjustment for age, sex, and fasting plasma glucose (HR [95% CI] per 1 SD = 0.76 [0.62; 0.94], p=0.012).Conclusion: We identified for the first time the mitochondrial-related biomarker IF1 as being associated with the risk of T2D.
Observing the photo-induced structural dynamics of proton transfer in o-nitrophenol by Ultrafast Electron Diffraction
Authors: Nunes J. Pedro F. , Williams Monika , Yang Jie , Wolf Thomas , Rankine Conor , Parrish Robert , Moore Bryan , Wilkin Kyle , Shen Xiaozhe , Lin Ming-Fu , Hegazy Kareem , Li Renkai , Weathersby Stephen , Martinez Todd , Wang Xijie , Centurion Martin 
Published: July 2022

Abstract
Many important biological and chemical processes are initiated by the transfer of a proton from a donor to an acceptor group within a molecule and are regulated by the interplay of nuclear and electronic dynamics. The electronic dynamics of proton transfer are well-studied, yet the nuclear dynamics have never been spatiotemporally resolved. Herein, we present a direct observation of the nuclear motions leading to and ensuing from photochemical proton transfer in o-nitrophenol using ultrafast electron diffraction. The proton transfer step is identified by the transient compression of the donor-acceptor distance and captured on the femtosecond timescale with sub-Angstrom resolution. Supported by ab initio multiple spawning simulations, our observations uncover the structural changes mediating excited-state relaxation. Our work provides an unprecedented mechanistic insight into the photochemical proton transfer mechanism and photo-relaxation dynamics of o-nitrophenol, unambiguously connecting an experimental observable to a process previously confined to theoretical models.