bluuebunny/crossref_metadata_2025_split huggingface dataset:
High CS Expression Leads to Poor Prognosis Associated with Immune Invasion of Lung Adenocarcinoma
Authors: Feng Ran , Hong Shihao , Bian Tingting , Gu Xue , Zheng Miaosen , Liu Jian , Liu Yifei 
Published: March 2022

Abstract
Background
Citrate synthase (CS) is the initial rate-limiting enzyme in tricarboxylic acid cycle. We found that its expression is up-regulated in many cancers. Previous studies on the relationship between the expression of CS and (LUAD) have not been reported till date.
Methods
we systematically analyzed several online databases, such as TCGA and GEO database, TIMER database, Kaplan Meier plotter, Ualcan database and Linkedomics database.
Results
we found that it was highly expressed in patients with LUAD. The high expression of CS caused poor prognosis in LUAD patients, and it was associated with some clinical traits. This indicates that it may be an independent prognostic factor. At the same time, we found that high CS expression was associated with the decline of immune-infiltrating B cells. While exploring the mechanism, we found that highly expressed CS mainly interacts with ATP5B and KPNB1 proteins. Moreover, CS is highly expressed in regulating the cell cycle, P53 binding site and oxygen binding pathways.
Conclusions
Our results confirmed CS was associated with LUAD and affected immune cell infiltration in LUAD. This provided a basis for further use of CS as a new therapeutic target and prognostic indicator of patients with LUAD.
Analytical prediction of cutting forces in cylindrical turning of 304 stainless steel using unequal division shear zone theory
Authors: Li BingLin , Zhang Rui 
Published: March 2022

Abstract
In order to establish a more accurate prediction model of turning forces, this paper proposed an analytical model for cylindrical turning with the consideration of the effect of the main cutting edge angle and the nose radius. Meanwhile, the unequal division shear zone theory in orthogonal free cutting is extended and applied to the oblique non-free cutting in the interaction between the chip units. To take into account the real tool nose geometry, the engaged part in the rounded nose is discretized into a set of cutting edge units. The geometrical parameters associated with the cutting edge units are analysed by using the coordinate transformation approach. Then, the improved oblique cutting model is developed for each cutting edge unit to acquire the component forces along the tool rake face. Finally, the resultant cutting forces in the turning process are calculated by the numerical integration method. To verify the effectiveness of the proposed model, the turning force experiment of 304 stainless steel was carried out by changing the cutting conditions, the main edge cutting angle and the nose radius. Through the comparative analysis between the measured results and the calculation values of the proposed model, it was found that the analytical prediction of cutting force is in good agreement with the experiment.
The first complete mitochondrial genome sequences for Ulidiidae and phylogenetic analysis of Diptera
Authors: xing kun , Kang Chen , Zhao Fei 
Published: March 2022

Abstract
Background: Tetanops sintenisi is a pest that mainly damages the roots of quinoa ( Chenopodium quinoa ) and it is first discovered in China in 2018. Methods and Results: Here, the complete mitochondrial genome (mitogenome) of T. sintenisi was sequenced and compared with the mitogenomes of other Diptera species. The results revealed that the mitogenome of T. sintenisi is 15763 bp in length (GenBank accession number: MT795181) and is comprised of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA genes, and a non-coding A+T-rich region (959 bp). The highly conserved gene arrangement of the mitogenome of T. sintenisi was identical to that of other Diptera insects. Twelve PCGs contained the typical insect start codon ATN, while cox1 had CGA as the start codon. The genes cox2 , nad4 , and nad1 contained an incomplete termination codon T; nad3 , nad5 , and cob contained the complete termination codon TAG; and the remaining seven PCGs contained the termination codon TAA. All tRNA genes were predicted to fold into the typical cloverleaf secondary structure. Phylogenetic analysis of 48 species based on the mitogenome sequence revealed that T. sintenisi clustered with the Tephritidae family, indicating that T. sintenisi and Tephritidae have a close phylogenetic relationship. Conclusions: The phylogenetic relationship of T. sintenisi based on the mitogenome was consistent with the traditional morphological taxonomy, according to which T. sintenisi belongs to the family Otitidae, which is closely related to the family Muscidae.
Drilling Fluid Properties Prediction: A Machine Learning Approach to Automate Laboratory Experiments
Authors: Aljubran Mohammad J. , AlBahrani Hussain I. , Ramasamy Jothibasu , Magana-Mora Arturo 
Published: March 2022

Abstract
Providing access to underground energy resources such as oil, gas, and geothermal energy requires drilling through subterranean rock formations. Drilling fluids are commonly used to enable this drilling process by serving a variety of functions. Chief among these is circulating into the drilled wellbore to remove the produced rock cuttings. Other functions include exerting the hydrostatic pressure necessary to prevent the flow of underground fluids into the wellbore while drilling, minimizing the invasion of solids and undesired fluids into the wellbore rock, and ensuring the fluid remains flowable within the means of the pumps available on site. To assess the ability of a drilling fluid to serve these functions, it has to have certain rheological properties, which are conventionally measured using specialized equipment. Considering that the formulation and components of these drilling fluids can vary greatly for different scenarios, the process of preparing samples and measuring their rheological properties, which is usually performed on a daily basis on a drilling site, is unavoidably time-consuming, repetitive, and error-prone. Based on this, it is apparent that there is a need for a computational model that can accurately predict drilling fluids properties based on the proposed concentration of their components without the need for further laboratory testing. This study describes a novel methodology to train a machine learning model derived from over 6,878 drilling fluid formulations to successfully predict water-based drilling fluids properties with a resulting R2 of 91.07 ± 6.35%.
Low FTO expression serves as a potential biomarker for diagnosis and prognosis of patients with liver cancer
Authors: Jiao Yan , Shi Hong Yun , Fu Chang , Quan Pan Pan , Zhang Li Yue 
Published: March 2022

Abstract
Background: As an important part of m6A modification, FTO plays a carcinogenic effect in cancer. In this study, we aim to explore whether FTO could be a new target for diagnosis and prognostic of patients with liver cancer.Material and Methods: We obtained and analysis data from TCGA database by using R software (version 3.5.1). The box plot was used to analyze the pattern of FTO expression. Receiver operating characteristic (ROC) curve was performed to evaluate diagnostic value of FTO expression in liver cancer. Fisher’s exact and chi-square tests were used to analyze relationship between FTO expression and clinical pathological characteristics. Kaplan-Meier curve and Cox regression analysis were used to explore the prognostic value of FTO in liver cancer.Results: We observed that FTO was down-regulated in liver cancer and was associated with patients’ age. ROC curve showed that FTO expression had good clinical diagnostic value. Our results also revealed that FTO expression was associated with age, histologic grade, M classification, vital status, and overall survival (OS) of patients. Low FTO expression decreased OS, and was an independent predictor of poor prognosis in patients with liver cancer.Conclusion: FTO could serves as a potential biomarker for the diagnosis and prognosis of liver cancer
Relationship between work-family conflict on work engagement in Chinese nurses: multiple mediating effects of anxiety and emotional intelligence
Authors: Chang Hongjuan , Liu Rui , Fang Xiaoxia , Li Xue 
Published: March 2022

Abstract
Background: In recent years, the work service quality of nurses has largely depended on their work engagement, which is extremely important and cannot be underestimated. Therefore, it is utmost importance to investigate the factors that can improve the work engagement of nurses, and the improvement in the level of work engagement of nurses is of great practical significance to the nursing service industry. A balance between work and family can promote the work engagement of nurses. This study aims to develop a structural model to assess the impact of work-family conflict on work engagement among Chinese clinical nurses using multiple mediators to clarify the underlying mechanisms of this relationship.Methods: This is a cross-sectional study involving 1,177 registered nurses from a hospital in China, which provided the work-family conflict, work engagement, anxiety, and emotional intelligence scales. AMOS 23.0 was used for the path analysis of multiple mediation models.Results: The total score of the work engagement of nurses was 58.49 ± 8.52, and the average score was 3.66 ± 0.53. Work-family conflict had a significant positive correlation with anxiety (r = 0.382, P < 0.001) and a significant negative correlation with work engagement (r = -0.381, P < 0.001) and emotional intelligence (r = -0.200, P < 0.001); anxiety had a significant negative correlation with work engagement (r = -0.326, P < 0.001) and emotional intelligence (r = -0.188, P < 0.001); and work engagement had a significant positive correlation with emotional intelligence (r = 0.326, P < 0.001). Emotional intelligence and anxiety partially mediated the relationship between work-family conflict and work engagement, with effect values of -0.092 and -0.075, respectively. The chain-mediating effect of anxiety and emotional intelligence exhibited a value of -0.05. The total indirect effect had a value of -0.172.Conclusions: Thus, it is suggested that hospital managers should take measures to alleviate work-family conflict, relieve anxiety, and improve the work engagement and emotional intelligence of nurses.
Simulated Interprofessional Education Program For Training Clinical Critical Thinking In Medical Nursing Students: A Mixed Method Study
Authors: Zhou Xin-yi , Wang Yan-feng , Dou Chun-xia , Tian Xiao-ying , Su Jin , Chen Yan-ya , Yan Feng-xia , Yang Qiao-hong , Wang Wenru 
Published: March 2022

Abstract
Background: Simulation teaching, which cultivates cooperation and communication in the interprofessional team, has become a suitable type of interprofessional cooperation in college students' health professional courses. This study aimed to explore the intervention effect of simulated interprofessional education (SIPE) on clinical critical thinking of medical nursing students. Methods: This study was a mixed method research including quantitative and qualitative assessment. Results: A total of 30 medical nursing students were randomly divided into a control group (18) and an experimental group (12). The mean scores on interprofessional cooperation and critical thinking of the experimental group were higher than those of the control group (P < 0.05). Quantitative evaluation was conducted using a scale, and qualitative evaluation was conducted through semi-structured interviews. There were three themes emerged: clarifying team positioning, improving team effectiveness, and optimizing the learning experience. Conclusion: This study found that the SIPE program improved medical nursing students' ability to work in interprofessional teams.
Control of the sandflies vector of leishmaniasis: Results of an entomological survey on the sandflies fauna in Morocco (El Hajeb)
Authors: El-Mouhdi Karima , Boussaa Samia , Fekhaoui Mohammed , Mehanned Smahane , Faraj Chafika , Chahlaoui Abdelkader 
Published: March 2022

Abstract
Background
Sandflies are small midges that are the exclusive vectors of cutaneous and visceral leishmaniasis. These diseases are a public health problem in Morocco. Objective: To determine the composition of the phlebotomine fauna, to study the biodiversity and seasonal distribution of phlebotomine in the province of El Hajeb.
Methods
A total of six stations were surveyed, five of which had recorded cases of indigenous leishmaniasis in recent years. Bi-monthly captures from April to December 2019 were carried out using adhesive trapping in different biotopes.
Results
A total of 14590 sand flies were collected of which males represent 80.3%. The activity of the sand fly population started in April and ended at the end of November. The periods of high abundance were in July, September and November. Morphological identification of fasting males and females (14070 specimens) shows the presence of twelve species belonging to the genus Phlebotomus and Sergentomyia: P. longicuspis (79.4%), P. sergenti (7.2%), S. minuta (4.42%), P. perniciosus (2.7%), P. papatasi (2.05%), P. bergeroti (1.1%), S. schwetzi (1%), P. alexandri (0.7%), S. fallax (0.7%), S. dreyfusi (0.5%), P. ariasi (0.2%) and S. antennata (0.1%). The in-depth analysis shows that the leishmaniasis vector species predominate the population of collected sandflies and the risk periods are spread during the summer and autumn seasons.
Conclusion
The present study is the result of an entomological survey at the level of El Hajeb in central Morocco; it gives information on the captured species of sandflies. Therefore, it offers to the decision-makers an important tool to carry out effective vector control actions to limit the risk of bites and to limit the transmission of leishmaniasis.
Implementation performance evaluation of hierarchical diagnosis and treatment system in China based on a sustainable perspective
Authors: Wu Qinde , Xie Xianyu 
Published: March 2022

Abstract
Hierarchical diagnosis and treatment system is essential for addressing the problem of 'difficult and expensive medical treatment'. But little research has focused on a sustainable perspective when evaluating the hierarchical diagnosis and treatment system performance. Using data from a total of 69 sample data in Fujian Province from 2018 to 2020 as representativeness, we explore the position of the hierarchical diagnosis and treatment policies, clarify the important restrictive factors presently, to better reflect the main contradictions in the Hierarchical Diagnosis and Treatment System in China. We find that the average value of the implementation performance of Hierarchical Diagnosis and Treatment System was > 0.5, which was at Level III. "Number of new technologies and new projects performed by hospitals and primary health technicians per 100 (B 3 )", "Number of people per 100 health technicians in the hospitals to the primary medical institutions for guidance (B 2 )", "Contracted coverage rate of family doctors (F 3 )" and "Proportion of master’s and doctor’s degrees of hospital health technicians (A6)" are important factors to promote the performance of Hierarchical Diagnosis and Treatment System at this stage. Our results suggest that policy makers need to smooth the communication and interaction channel of professional disciplines and fully play to the role of medical information technology, pay attention to the construction of family doctors and improve the service level of first diagnosis at the primary level, and improve the medical service capacity of the hospitals through supplement high-quality medical personnel.
Angiectatic nasal polyps with pleomorphism- a diagnostic pitfall
Authors: Zhao Xiaoli , Yue Changli , Xing Li , Li Rui , zhang Yong , Yin Hongyan , Liu Honggang , Piao Yingshi 
Published: March 2022

Abstract

Background Angiectatic nasal polyps with pleomorphism (PANP) are rare benign lesions of nose and paranasal sinuses. Clinical manifestation includes nose obstruction, bleeding and headache. The demonstrating bone-damaged lesion by imaging as well as the enlarged and pleomorphic stromal cells with nuclear atypia by morphology simulate a malignant process, which may be misdiagnosed. The aim of the present study is to generalise the features of PANP in case of potential clinical and pathological pitfall of diagnosis. Methods Thirteen patients diagnosed as PANP were retrospectively analyzed in the Pathology Department of Beijing Tongren Hospital Affiliated to Capital Medical University from August 2014 to December 2019. Immunohistochemical staining with CD34, CK, Vim, Calponin, Ki67,Bcl-2, and STAT-6 was performed with envision-two steps method.Results PANP is a benign tumor presenting with gross variegated tan to gray soft fleshy tissue with foci of obvious hemorrhage and necrosis. The imaging shows internal heterogeneous hyperintensity with a peripheral hypointense rim while postcontrast images display a strong nodular and patchy enhancement. Vimentin (Vim) stain was consistently positive , while negative for CD34, STAT-6 and Bcl-2 (focal positive in two cases). Calponin and CK stain was positive in nine cases, respectively . Conclusions PANP is a clinically rare tumor which may stimulate malignancy lesion. Recognizing of characteristic features in these thirteen patients would be beneficial to avoid misdiagnosis and unnecessary aggressive treatment.
Molecular Response Mechanism of Pseudomonas Nicosulfuronedens LAM1902 to A Typical Sulfonylurea Herbicide Nicosulfuron
Authors: Li Miaomiao , Li Qingqing , Yao Jun , Sunahara Geoffrey , Duran Robert , Zhang Qinghua , Ruan Zhiyong 
Published: March 2022

Abstract
The overuse of the herbicide nicosulfuron (NS) has become a global environmental concern. As a potential bioremediation technology, the microbial degradation of NS shows much promise; however, the detailed mechanisms of microbial taxa responding to NS exposure require further study. An isolated soil-borne bacteria Pseudomonas nicosulfuronedens LAM1902 displaying NS degradabilities was used to characterize the molecular responses to NS exposure, and LAM1902 can degrade exceed 95% of 50 mg/L nicosulfuron under the optimal conditions. Using transcriptomic sequencing, RNA-Seq results indicated that 1102 differentially expressed genes (DEGs) were up-regulated and 702 down-regulated in response to NS. Among them, “ABC transporters,” “sulfur metabolism,” and “ribosome” gene pathways were significantly enriched (p ≤ 0.05). Several gene pathways involved in the glycolysis and pentose phosphate pathways, a two-component regulation system, as well as in bacterial chemotaxis metabolisms were up-regulated under NS exposure. Surprisingly, NS exposure showed positive effects on the production of oxalic acid that is synthesized by genes encoding glycolate oxidase through the glyoxylate cycle pathway. The results suggest that P. nicosulfuronedens LAM1902 adopt acid metabolites production strategies in response to NS, with concomitant NS degradation. Meanwhile, strain LAM1902 likely grows and survives amid NS stress by increased energy production. The present studies provide a glimpse at the molecular response of microorganisms to sulfonylurea pesticide toxicity and a potential framework for future mechanistic studies.
TEDC2 correlated with prognosis and immune microenvironment in lung adenocarcinoma
Authors: Fang Likui , Yu Wenfeng , Zhu Pengfei , Yu Guocan , Ye Bo 
Published: March 2022

Abstract
Tubulin epsilon and delta complex 2 (TEDC2) is a protein coding gene which function is poorly identified yet. This study aimed to identify the role of TEDC2 in prognosis and immune microenvironment of lung adenocarcinoma (LUAD). Through The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, the mRNA expression of TEDC2 was upregulated in LUAD tissues compared to normal tissues. The protein level of TEDC2 was also higher in LUAD in the Human Protein Atlas. The receiver operating characteristic (ROC) curve showed that high TEDC2 level could distinguish LUAD patients from normal subjects. In addition, the impact of TEDC2 expression on prognosis was evaluated by Kaplan-Meier and Cox regression analyses, and the results suggested that high TEDC2 expression was significantly associated with poor prognosis and was the independent prognostic factor in LUAD. GO and KEGG pathway analyses indicated the co-expressed genes of TEDC2 were mainly related to mitotic cell cycle processes. Importantly, high expression of TEDC2 indicated low infiltration of immune cells, especially dendritic cells and B cells. TEDC2 was also positively correlated with immune checkpoints such as PDCD1, LAG3 and CD276. Taken together, this study preliminarily revealed the clinical significance of TEDC2 in LUAD and provided novel insights into the role of TEDC2 in immune microenvironment.
Gliosarcoma with Extensive Extracranial Metastatic Spread and Familial Coincidence: An Illustrative Case
Authors: Friker Lea L. , Tzaridis Theophilos , Enkirch Simon Jonas , Lüders Christine , Hattingen Elke , Kristiansen Glen , Goschzik Tobias , Waha Andreas , Lütter Christiana , Weller Johannes , Herrlinger Ulrich , Pietsch Torsten , Gessi Marco , Baumert Brigitta , Gielen Gerrit H. 
Published: March 2022

Abstract
Gliosarcoma is a rare histopathological subtype of glioblastoma. Metastatic spreading is very unusual. In this report, we illustrate a case of gliosarcoma with extensive extracranial metastases with confirmation of histological and molecular concordance between the primary tumor and a metastatic lesion of the lung. Moreover, the case revealed a family coincidence of glial tumors as the patient’s son was diagnosed with a high-grade glioma shortly after the patient’s death. By molecular analysis (Sanger and next generation panel sequencing), we could confirm that both patients carried mutations in the TP53 gene. Interestingly, the detected mutations were located in different exons. According to the current scientific knowledge, even further genetic findings do not explain the aggressive metastatic spread observed in the reported case nor reveal a family tumor syndrome. In conclusion, the molecular mechanisms leading to a metastatic phenotype of gliosarcoma remain unknown and should be studied further.
Evaluation of the Use of GRADE in Dentistry Systematic Reviews and its Impact on Conclusions: A Protocol for a Methodological Study
Authors: Ibrahim Sara , Azab Maria , Miroshnychenko Anna , Brignardello-Petersen Romina 
Published: March 2022

Abstract
Background: There is a growing body of evidence of systematic reviews (SRs) with varying degrees of methodological quality. The Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach allows SR authors to assess the certainty of the evidence they found and transparently relay their conclusions. As there appears to be infrequent utilization of GRADE in the field of dentistry, to identify the impact of GRADE, the aim of this study is to evaluate the use of GRADE in the dental literature and determine whether SRs that use GRADE differ from those that do not with respect to their conclusions.Methods/Design: We will search Ovid MEDLINE for SRs published from 2016 to the present. We will conduct both screening and data extraction independently and in duplicate and use pre-piloted, standardized forms for data extraction. We will determine the frequency of the use of GRADE and the varying levels of certainty in the current literature and evaluate whether GRADE is being used appropriately. We will also evaluate whether SRs not using GRADE differ from those that use GRADE with regards to methodological quality. We will also determine whether the conclusions of SRs that do not use GRADE would change had GRADE been utilized. Additionally, we will evaluate whether SRs using GRADE are more likely to formulate appropriate conclusions compared to SRs that do not use it.Discussion: This study will investigate the frequency of GRADE assessments in dentistry SRs and the impact of GRADE assessments on the conclusions of a SR. It has important implications for both SR authors and users of this type of literature.