bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic profiling and biochemical analysis of stored Hass avocado fruit by GC‐MS and UHPLC‐UV‐VIS revealed oxidative stress as the main driver of ‘blackspot’ physiological disorder
Authors: Uarrota Virgilio G. , Hernández Ignacia , Ponce Excequel , Bauer Claudia M. , Maraschin Marcelo , Pedreschi Romina 
Published: October 2022

SummaryThe goal of this study was to use GC‐MS and UHPLC‐UV‐VIS‐based metabolomic tools combined with chemometric analysis to discriminate between fruit with blackspot physiological disorder independently of storage condition (regular air or controlled atmosphere) and without blackspot (hereafter referred to as ‘Control’). The study looked at polar metabolites, enzymes, fatty acids and oxidative stress markers. Fruit with high levels of lenticel damage are more likely to develop blackspot, which appears to be exacerbated by H2O2 and peroxidation of lipids. Lower catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO), superoxide dismutase (SOD) and phenylalanine‐ammonia lyase (PAL) activities together with lower levels of polyphenols (syringic acid, epigallocatechin, quercetin and vanillic) and fatty acids (linolenic, linoleic and palmitic acid), as well as ß‐sitosterol, methyl‐3‐hydroxybutyrate, glyceric acid, hydroxyl butanoic acid, L‐threonic acid, hydroxyl glutaric acid and xylose contributed to a higher prevalence of blackspot disorder in stored Hass avocados, which was accompanied by upregulation of soluble proteins. Finally, chemometric analysis revealed a positive relationship between blackspot disorder with gluconic, palmitoleic, galactose oxime, glyceric, hydroxyglutaric, MDA, syringic and oleic acid, as well as a negative relationship with catechin, epigallocatechin, SOD, CAT and tagatose. The study concluded that oxidative stress is the main driver of ‘Blackspot’ physiological disorder.
An immunogenetic investigation of 30 autoimmune and autoinflammatory diseases and their links to psychiatric disorders in a nationwide sample
Authors: Nudel Ron , Allesøe Rosa Lundbye , Werge Thomas , Thompson Wesley K. , Rasmussen Simon , Benros Michael E. 
Published: October 2022

AbstractAutoimmune and autoinflammatory diseases (AIIDs) involve a deficit in an individual's immune system function, whereby the immune reaction is directed against self‐antigens. Many AIIDs have a strong genetic component, but they can also be triggered by environmental factors. AIIDs often have a highly negative impact on the individual's physical and mental wellbeing. Understanding the genetic underpinning of AIIDs is thus crucial both for diagnosis and for identifying individuals at high risk of an AIID and mental illness as a result thereof. The aim of the present study was to perform systematic statistical and genetic analyses to assess the role of human leukocyte antigen (HLA) alleles in 30 AIIDs and to study the links between AIIDs and psychiatric disorders. We leveraged the Danish iPSYCH Consortium sample comprising 65 534 individuals diagnosed with psychiatric disorders or selected as part of a random population sample, for whom we also had genetic data and diagnoses of AIIDs. We employed regression analysis to examine comorbidities between AIIDs and psychiatric disorders and associations between AIIDs and HLA alleles across seven HLA genes. Our comorbidity analyses showed that overall AIID and five specific AIIDs were associated with having a psychiatric diagnosis. Our genetic analyses found 81 significant associations between HLA alleles and AIIDs. Lastly, we show connections across AIIDs, psychiatric disorders and infection susceptibility through network analysis of significant HLA associations in these disease classes. Combined, our results include both novel associations as well as replications of previously reported associations in a large sample, and highlight the genetic and epidemiological links between AIIDs and psychiatric disorders.
SiC nanograins stabilized Si–C–B–N fibers with ultrahigh‐temperature resistance
Authors: Tian Yuan , Long Xin , Shao Changwei , Wang Yingde 
Published: October 2022

AbstractContinuous ceramic fibers with ultrahigh‐temperature stability are in high demand for applications in advanced space propulsion and thermal protection systems. In this study, SiC nanograins stabilized Si–C–B–N ceramic fibers were prepared using chemically modified polyborosilazane via a polymer‐derived method. The fabricated Si–C–B–N fibers exhibited a rather high tensile strength of approximately 1.8 GPa and a high strength retention of approximately 90% after annealing at 2100°C for 0.5 h under a nitrogen atmosphere. The ultrahigh‐temperature stability can be contributed to the presence of thermodynamically stable SiC nanograins and the encapsulation of SiC nanograins by the BN(C) phase and amorphous Si–C–B–N matrix. Our work offers a convenient strategy for preparing Si‐based ceramic fibers with ultrahigh‐temperature stability at beyond 2000°C.
Superiority of complete bi‐atrial ablation procedure for atrial fibrillation in rheumatic mitral valve disease
Authors: Li Haojie , Yu Chunyu , Gao Ge , Wang Shuiyun , Liu Sheng , Wang Xianqiang , Zheng Zhe 
Published: October 2022

AbstractIntroductionThe necessity of complete bi‐atrial lesion created by radiofrequency clamp and pen for nonparoxysmal atrial fibrillation (AF) in patients with rheumatic mitral valve disease (RMVD) remains unclear.MethodsFrom January 2014 to December 2018, patients with RMVD concomitant with nonparoxysmal AF who underwent mitral valve surgery concomitant surgical ablation were retrospectively enrolled. We divided patients into Group A (complete bi‐atrial lesion set created by radiofrequency clamp and pen) and Group B (simplified lesion sets created by radiofrequency clamp alone including bi‐atrial ablation with incomplete mitral isthmus line and stand‐alone left atrial ablation) according to the surgical ablation lesion sets. Propensity score matching was applied to analyze freedom from atrial tachyarrhythmias between the two groups.ResultsTwo hundred eight (38.5%) and 332 (61.5%) patients were divided into Group A and Group B, respectively. In Group B, the proportion of patients with recurrent atrial flutter in the subgroup of bi‐atrial ablation with incomplete mitral isthmus line was higher than that in Group A (p = .044). After propensity score matching, there were 203 patients in each group. Better freedom from atrial tachyarrhythmias without antiarrhythmic drugs was obtained in Group A (83.1%, 79.6%, and 65.4%) than Group B (73.1%, 68.4%, and 52.7%) at 12, 36, and 60 months after operation (p = .012).ConclusionThe application of radiofrequency clamp and pen to create complete bi‐atrial lesion set in surgical ablation for nonparoxysmal AF in RMVD was associated with superior long‐term efficacy.
Review of the spotted lizard loaches, Pseudohomaloptera (Cypriniformes: Balitoridae) with a re‐description of Pseudohomaloptera sexmaculata and description of a new species from Sumatra
Authors: Randall Zachary S. , Somarriba Gabriel A. , Tongnunui Sampan , Page Lawrence M. 
Published: October 2022

AbstractA review of the six recognized species of Pseudohomaloptera is provided. Counts in the original description of Pseudohomaloptera sexmaculata Fowler (1934) were incorrect and led to confusion in identifying populations of Pseudohomaloptera in mainland Southeast Asia, and the species is re‐described. The validity of Homaloptera septemmaculata Fowler (1934) is investigated and confirmed as a junior synonym of P. sexmaculata. P. sexmaculata and Pseudohomaloptera leonardi, similar morphologically and often misidentified, are widely distributed in mainland Southeast Asia, with P. sexmaculata in the Chao Phraya, Mae Klong and Pran Buri River basins, and P. leonardi in the Malay Peninsula and the Chao Phraya and Mekong River basins. Pseudohomaloptera yunnanensis and Pseudohomaloptera vulgaris have been reported from the Mekong basin of Thailand and Laos but appear to be restricted to Yunnan Province, China. A new species of Pseudohomaloptera is described from Sumatra. This is the southern‐most species and first record for the genus from the Indonesian island. An identification key is provided for all species of the genus.
Multifamily therapy in the community mental health network: A pragmatic randomized and controlled study
Authors: Domínguez Vázquez Iria , Nuño Gómez Laura , Freixa Fontanals Neus , Hidalgo Arjona Carmen , Cervera Gemma , Corominas Díaz Antoni , Balcells‐Oliveró Mercè 
Published: October 2022

AbstractMultifamily therapy (MFT) is a psychotherapeutic group intervention for patients with severe mental disorders (SMDs) and their families. The present study is a multicenter, randomized, and controlled trial that analyzes the benefit of MFT during outpatient treatment. The recruited patients were randomly assigned to the experimental group (n = 26), which received 24 MFT sessions in addition to their treatment as usual (TAU), or to the control group (n = 29), which received only TAU (individual and family sessions). Six months after the inclusion in the MFT, the experimental group showed a significant decrease in number of visits to the psychiatric emergency services, number of psychiatric admissions, and the days of admission. The need for hospital care 6 months after recruitment was also lower in the experimental group compared to the control group. These results suggest that the implementation of MFT during outpatient treatment facilitates community management of people diagnosed with mental health problems.
Comparison of mouth rinsing performance between adults and children using a contactless vital sensing camera
Authors: Kanada Hisako , Yamamoto Yushi , Sato Hideo , Chinju Kohei , Ariyasu Yuichi , Kawaji Maria , Iwashita Yoichiro , Hashiguchi Makiko , Yamasaki Youichi 
Published: October 2022

AbstractBackgroundEvaluating mouth rinsing skills is useful for assessing oral function, however current evaluation methods are subjective.ObjectivesThis study compared mouth rinsing between adults and children using a contactless camera to capture lip motion.MethodsThe subjects comprised 16 adults and 13 children with no oral dysfunction. A compact vital sensing camera adapted from a Microsoft Xbox One Kinect Sensor® (Kinect) was placed 100 cm from the floor and 120 cm from the subject; 5, 10 and 15 ml of water were used as samples. Participants were instructed to hold the sample in the oral cavity, close the lips and move the sample alternatively left and right for 15 s. Maximum/minimum displacement from the reference plane and rinsing cycle for each sample were analysed by one‐way analysis of variance.ResultsIn adults, there was no significant difference in the maximum/minimum displacement between the left and right sides of the angulus oris due to differences in sample amount. In children, the right maximum significantly differed between the 5‐ and 15‐ml and 10‐ and 15‐ml samples, while the left maximum significantly differed between the 5‐ and 10‐ml and 5‐ and 15‐ml samples. The right minimum significantly differed between the 5‐ and 10‐ml samples, as did the duration of mouth rinsing between the 5‐ and 15‐ml samples.ConclusionsIn children, lip movement and mouth rinsing duration tended to decrease with increasing sample volume. Evaluating lip movement using a contactless vital sensing camera is useful for assessing children's development of oral function.
Silmitasertib, a casein kinase 2 inhibitor, induces massive lipid droplet accumulation and nonapoptotic cell death in head and neck cancer cells
Authors: Su Ying‐Wen , Huang Wen‐Yu , Lin Huan‐Chau , Liao Po‐Nien , Lin Ching‐Yung , Lin Xiang‐Yu , Huang Sing‐Han , Chen Yi‐Ting , Wu Pao‐Shu 
Published: October 2022

AbstractBackgroundAccumulating evidence shows that high expression of casein kinase 2 (CK2) and phosphorylated acetyl CoA carboxylase (pACC) in patients with squamous cell carcinoma of the head and neck (SCCHN) correlates with decreased survival rates. Computational analysis has shown that ACC is a potential substrate for CK2, and its inhibition can suppress ACC phosphorylation in vitro. CX‐4945, also known as silmitasertib, is an orally administered, highly specific, ATP‐competitive inhibitor of CK2 and is under clinical investigation as a treatment for malignancies. We hypothesize that inhibition of CK2 by CX‐4945 can reduce CK2‐downstream phosphorylation of ACC as a therapeutic strategy against SCCHN.MethodsThree aggressive SCCHN cell lines (OSC‐19, FaDu and HN31) were cultured to investigate the anticancer mechanism of the CK2 inhibitor, CX‐4945. Cell cycle analysis, Annexin V/PI staining, and cleavage of PARP were performed to detect apoptosis. Western blot, electron microscopy and analysis of acidic vesicular organelle development were used to detect autophagy. Interference with cellular metabolism by CX‐4945 treatment was determined by Seahorse XF24 Extracellular Flux Analyzer and mass spectrometry.ResultsCellular metabolism was impeded by CX‐4945 in aggressive SCCHN cells by Seahorse XF24 Extracellular Flux Analyzer and mass spectrometry, and consequently time‐ and dose‐dependent lipid droplet accumulation and non‐apoptotic cell death were observed. The lipogenic enzyme ACC was demonstrated to be associated with CK2, and its repressive phosphorylation could be removed by the CK2 inhibitor CX‐4945. Overexpression of ACC resulted in impaired cell survival following transient transfection.ConclusionThe findings demonstrate that CK2 inhibition impairs normal cellular energy metabolism and may be an attractive therapy for treating aggressive SCCHN.
Beyond the brain localization of complex traits: Distributed white matter markers of personality
Authors: Stendel Moriah S. , Chavez Robert S. 
Published: October 2022

AbstractObjectiveExtensive work in personality neuroscience has shown mixed results in the ability to localize reliable relationships between personality traits and neuroimaging measures. However, recent work in translational neuroimaging has recognized that multifaceted psychological dispositions are not represented in discrete, highly localized brain areas. As such, standard univariate neuroimaging analyses may not be well‐suited for capturing broad personality traits supported by distributed networks.MethodThe present study uses an out‐of‐sample predictive modeling approach to identify multivariate signatures of Big Five personality traits within the structural integrity of white matter pathways using diffusion magnetic resonance imaging. In Study 1 (N = 491), we trained a ridge regression model to predict each of the Big Five traits and tested these models in an independent hold‐out subsample.ResultsWe found that models for both Neuroticism and Openness were significantly related to predictive accuracy in the hold‐out sample. Study 2 (N = 108) applied Study 1's predictive models to an independent set of data collected on a different scanner and using a different Big Five scale. Here, we found that the model for Neuroticism remained a significant predictor of individual difference.ConclusionOur findings provide evidence that this white matter signature of Neuroticism generalizes across differences in measurement and samples.
53Mn‐53Cr chronology and ε54Cr‐Δ17O genealogy of Erg Chech 002: The oldest andesite in the solar system
Authors: Anand Aryavart , Kruttasch Pascal M. , Mezger Klaus 
Published: October 2022

AbstractThe meteorite Erg Chech (EC) 002 is the oldest felsic igneous rock from the solar system analyzed to date and provides a unique opportunity to study the formation of felsic crusts on differentiated protoplanets immediately after metal–silicate equilibration or core formation. The extinct 53Mn‐53Cr chronometer provides chronological constraints on the formation of EC 002 by applying the isochron approach using chromite, metal–silicate–sulfide, and whole‐rock fractions as well as “leachates” obtained by sequential digestion of a bulk sample. Assuming a chondritic evolution of its parent body, a 53Cr/52Cr model age is also obtained from the chromite fraction. The 53Mn‐53Cr isochron age of 1.73 ± 0.96 Ma (anchored to D'Orbigny angrite) and the chromite model age constrained between and Ma after the formation of calcium‐aluminium‐rich inclusions (CAIs) agree with the 26Al‐26Mg ages (anchored to CAIs) reported in previous studies. This indicates rapid cooling of EC 002 that allowed near‐contemporaneous closure of multiple isotope systems. Additionally, excess in the neutron‐rich 54Cr (nucleosynthetic anomalies) combined with mass‐independent isotope variations of 17O provides genealogical constraints on the accretion region of the EC 002 parent body. The 54Cr and 17O isotope compositions of EC 002 confirm its origin in the “noncarbonaceous” reservoir and overlap with the vestoid material Northwest Africa 12217 and anomalous eucrite Elephant Moraine 92023. This indicates a common feeding zone during accretion in the protoplanetary disk between the source of EC 002 and vestoids. The enigmatic origin of iron meteorites remains still unresolved as EC 002, which is more like a differentiated crust, has an isotope composition that does not match known iron meteorite groups that were once planetesimal cores.
Comparative transcriptome analysis reveals an insight into the candidate genes involved in anthocyanin and scent volatiles biosynthesis in colour changing flowers of Combretum indicum
Authors: Ghissing U. , Kutty N. N. , Bimolata W. , Samanta T. , Mitra A. 
Published: October 2022

ABSTRACT

Combretum indicum is a widely cultivated ornamental species displaying the distinct phenomenon of floral colour change. Flowers display a gradual colour change from white to red, attributed to increased cyanidin 3‐O glucoside in petal tissues. The differently coloured flowers also emanate a complex blend of VOCs with trans‐linalool oxide (furanoid) as the major compound in the emission profile.

To understand molecular mechanisms regulating floral colour shifts and scent biosynthesis, we performed Illumina transcriptome sequencing, including de novo assembly and functional annotation, for the two stages of floral maturation (white and red). Homology analysis with functional classification identified 84 and 42 candidate genes associated with pigment and scent biosynthesis, respectively. Genes encoding transcription factors, such as MYB, ERF, WD40, WRKY, NAC, bHLH and bZIP, that play critical roles in regulating specialized metabolism were also identified in the transcriptome data.

Differences in expression of genes were consistent with accumulation patterns of anthocyanins in the two different flower colours. A clear upregulation of flavonoid biosynthesis genes in red flower tissue is associated with increased pigment content. RT‐qPCR‐based expression analyses gave results consistent with the RNA‐Seq data, suggesting the sequencing data are consistent and reliable.

This study presents the first report of genetic information for C. indicum. Gene sequences generated from RNA‐Seq, along with candidate genes identified by pathway mapping and their expression profiles, provide a valuable resource for subsequent studies towards molecular understanding of specialized metabolism in C. indicum flowers.
Gaining by ceding ‐ bounded wholesale pricing for information sharing in a supply chain
Authors: Li Tian , Zhang Hongtao 
Published: October 2022

The extant research on supply chain information sharing under wholesale pricing often assumes a model where the manufacturer can unilaterally set any wholesale price, and the retailer decides retail quantity or price while taking the wholesale price as given. Whereas this model may actually reflect the relative market power in some situations, its implementation misses out on certain win–win opportunities that could arise from information sharing. We propose a new wholesale pricing mechanism that promotes information sharing between a relatively weaker retailer and a more powerful manufacturer such that both parties become better off. The novel feature of the new mechanism is an extra stage of interaction, which happens before the retailer's information sharing decision: at the very outset the manufacturer and the retailer reach a contractual agreement on an upper bound which the wholesale price will not exceed if the retailer shares his information. This new, easily implementable, mechanism can be a win–win for both supply chain firms. In other words, facing market uncertainty, the manufacturer, while having the market power to dictate the wholesale price, can gain from a contractual restriction on the exercise of that power in order to induce the retailer, who is in a weaker position, to share useful demand information.
Oral environment and cerebral blood flow in patients with neurodegenerative dementia: comparison of Alzheimer type dementia and dementia with Lewy bodies
Authors: Inoue Makoto , Kimoto Katsuhiko , Honma Yuta , Tomita Rintaro , Manabe Yuta 
Published: October 2022

BackgroundThe number of patients with dementia is increasing worldwide. Modifying risk factors may significantly reduce the prevalence and cost of dementia. The number of remaining teeth, occlusal force, denture use, and periodontal disease status are associated with the risk of developing dementia. The oral environment may be a risk factor for dementia. This study aimed to investigate the association between the oral environment and cerebral blood flow in patients with mild cognitive impairment and dementia (either Alzheimer type dementia (ATD) or dementia with Lewy bodies (DLB)). We aimed to identify differences in the oral environment according to the underlying neurodegenerative disease.MethodsThe participants were 25 patients with ATD and 25 patients with DLB who visited the Department of Dementia and Geriatric Medicine, Kanagawa Dental University Hospital.Patients were diagnosed with ATD and DLB by well‐trained specialists certified by the Japanese Dementia Society and categorised as Level 3 or 4 by the Functional Assessment Staging of Alzheimer Disease scale. The correlation between oral environment (number of teeth, periodontal tissue, and oral function) and cerebral blood flow in each brain area calculated by single photon emission computed tomography was examined.ResultsThe DLB group showed a significant correlation between masticatory performance and cerebral blood flow in some areas, while no such correlation was observed in the ATD group. Significant correlations were found between periodontal pocket depth and bleeding on probing and cerebral blood flow in some areas in both groups, although the correlation was considerable in the ATD group.ConclusionsThe results of this study suggest that the association between changes in the oral environment and cerebral blood flow may differ among patients with ATD and DLB, depending on the underlying causative disease.
Activation through marketisation as a process of ignorancing
Authors: Roche Zach , Griffin Ray 
Published: October 2022

AbstractThis article explores the rationalities and mechanisms of outsourcing public employment service activation casework. We draw on textual and documentary data from minutes of meetings and inspection reports between the Irish Government and two market providers, over 7 years, to better understand how activation through marketisation unfolds in practice. We identify how the outsourcing contract unleashing a process of ignorancing (agnotology), where the Government wilfully and strategically seeks to overwrite existing forms of street‐level bureaucratic knowledge and practice. Our analysis reveals how activation through marketisation unfolds, first as strategic ignorance, then as involuntary ignorance as Government officials and contractors become bound by the contract until finally, the contract generates naturalised ignorance–new caseworkers without the basic knowledge, education, and training to support unemployed people. In this way, we offer a deeper understanding of the administration of activation through marketisation policies.