bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agaricus blazei Murill extract‐loaded in alginate/poly(vinyl alcohol) films prepared by Ca2+ cross‐linking for wound healing applications
Authors: Campelo Matheus da Silva , Mota Lucas Barroso , Câmara Neto João Francisco , Barbosa Maria Lucianny Lima , Gonzaga Maria Leônia da Costa , Leal Luzia Kalyne Almeida Moreira , Bastos Maria do Socorro Rocha , Soares Sandra de Aguiar , Ricardo Nágila Maria Pontes Silva , Cerqueira Gilberto Santos , Ribeiro Maria Elenir Nobre Pinho 
Published: December 2022

AbstractThis work aimed the development and evaluation of the wound healing activity of films based on sodium alginate, polyvinyl alcohol (PVA) and Ca2+ loaded with Agaricus blazei Murill hydroalcoholic extract (AbE). Firstly, AbE was prepared using a previously standardized methodology. The films were prepared by casting technique and cross‐linked with Ca2+ using CaCl2 as cross‐linking agent. The physicochemical, morphological and water vapor barrier properties of the films were analyzed and the pre‐clinical efficacy was investigated against the cutaneous wound model in mice. The films showed barrier properties to water vapor promising for wound healing. AbE showed physical and chemical interactions between both polymers, noticed by Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, and thermal analysis. The delivery of AbE in alginate/PVA films enhanced the antioxidant and wound healing properties of these polymers. Consequently, a reduction of malondialdehyde levels was observed, as well as an increase of the epidermis/dermis thickness and enhancement in collagen I deposition. Thus, these formulations are promising biomaterials for wound care and tissue repairing.
Cannabinoids inhibit ethanol‐induced activation of liver toxicity in rats through JNK/ERK/MAPK signaling pathways
Authors: Yan Lei , Luo Heng , Tang Xiaolu , Wang Haidong 
Published: December 2022

AbstractCannabinoids (CBs) are psychoactive compounds, with reported anticancer, anti‐inflammatory, and anti‐neoplastic properties. The study was aimed at assessing the hepatoprotective effects of CB against ethanol (EtOH)‐induced liver toxicity in rats. The animals were divided into seven groups: control (Group I) and Group II were treated with 50% ethanol (EtOH 5 mg/kg). Groups III, IV, and VI were treated with (EtOH + CB 10 mg/kg), (EtOH + CB 20 mg/kg), and (EtOH + CB 30 mg/kg), respectively. Groups V and VII consisted of animals treated with 20 and 30 mg/kg, of CB, respectively. Biochemical analysis revealed that Group IV (EtOH + CB 20 mg/kg) had reduced levels of ALT—alanine transferase, AST—aspartate aminotransferase, ALP—alanine peroxidase, MDA—malondialdehyde and increased levels of GSH‐reduced glutathione. Histopathological analysis of liver and kidney tissues showed that EtOH + CB (20 and 30 mg/kg) treated animal groups exhibited normal tissue architecture similar to that of the control group. ELISA revealed that the inflammatory markers were reduced in the animal groups that were treated with EtOH + CB 20 mg/kg, in comparison to the animals treated only with EtOH. The mRNA expression levels of COX‐2, CD‐14, and MIP‐2 showed a remarkable decrease in EtOH + CB treated animal groups to control groups. Western blot analysis revealed that CB downregulated p38/JNK/ERK thereby exhibiting its hepatoprotective property by inhibiting mitogen‐activated protein kinase pathways. Thus, our findings suggest that CB is a potential candidate for the treatment of alcohol‐induced hepatotoxicity.
DDIT4 promotes malignancy of head and neck squamous cell carcinoma
Authors: Zhang Zhenxing , Zhu Haoran , Zhao Chifeng , Liu Dong , Luo Jun , Ying Yukang , Zhong Yuan 
Published: December 2022

AbstractThis study investigated the cancer‐promoting effect of ferroptosis regulator DNA damage‐inducible transcript 4 (DDIT4) and its relevant mechanisms. Vital ferroptosis‐related genes were identified using bioinformatic methods on the basis of data collected from TCGA and seven other online databases. Cell Counting Kit‐8 (CCK8), colony formation, wound‐healing and transwell assays, and western blot analysis were conducted for verifying the biological role of DDIT4 in vitro. The immune score and tumor purity were calculated using R package “estimate.” The relationship was identified between DDIT4 expression and immune cell infiltration using ssGSEA and CIBERSORT algorithms. R package “Seurat” was used to perform unsupervised clustering of the single cells, and “SingleR” was utilized for annotation. R package “STUtility” was employed to plot the spatial expression of DDIT4. For trajectory analysis, monocle was used to predict cell differentiation and demonstrate the expression of DDIT4 at each state. Here, DDIT4 overexpression was observed in Head and Neck Squamous Cell Carcinoma (HNSCC) cohort, and DDIT4 upregulation showed a positive correlation with larger tumor size, lymph node metastasis, more advanced TNM stage and higher tumor mutational burden (TMB). Moreover, DDIT4 knockdown could markedly inhibit the proliferation, colony formation, invasion and migration of HNSCC cells, as well as suppress the expression of HIF‐1a, VEGF and vimentin. In comparison, DDIT4 overexpression showed a negative correlation with immune score and infiltrations of several immune cells. DDIT4 played crucial roles in the differentiation of CAFs and T cells. Collectively, this study demonstrates that DDIT4 contributes a critical role in HNSCC progression. The positive feedback regulation between DDIT4 and HIF‐1a may be a potential target for HNSCC treatment.
Somatic A‐to‐I RNA‐edited RHOA isoform 2 specific‐R176G mutation promotes tumor progression in lung adenocarcinoma
Authors: Chen Kuan‐Ju , Huang Jing‐Hsiang , Shih Jou‐Ho , Gu De‐Leung , Lee Szu‐Shuo , Shen Roger , Hsu Yu‐Hsuan , Kung Ying‐Chih , Wu Cheng‐Yen , Ho Chun‐Ming , Jen Hsin‐Wei , Lee Hsin‐Yi , Lang Yaw‐Dong , Hsiao Chen‐Hao , Jou Yuh‐Shan 
Published: December 2022

AbstractAdenosine‐to‐inosine (A‐to‐I) RNA editing is the most common posttranscriptional editing to create somatic mutations and increase proteomic diversity. However, the functions of the edited mutations are largely underexplored. To identify novel targets in lung adenocarcinoma (LUAD), we conducted a genome‐wide somatic A‐to‐I RNA editing analysis of 23 paired adjacent normal and LUAD transcriptomes and identified 26,280 events, including known nonsynonymous AZIN1‐S367G and novel RHOAiso2 (RHOA isoform 2)‐R176G, tubulin gamma complex associated protein 2 (TUBGCP2)‐N211S, and RBMXL1‐I40 M mutations. We validated the edited mutations in silico in multiple databases and in newly collected LUAD tissue pairs with the SEQUENOM MassARRAY® and TaqMan PCR Systems. We selected RHOAiso2‐R176G due to its significant level, isoform‐specificity, and being the most common somatic edited nonsynonymous mutation of RHOAiso2 to investigate its roles in LUAD tumorigenesis. RHOAiso2 is a ubiquitous but low‐expression alternative spliced isoform received a unique Alu‐rich exon at the 3′ RHOA mRNA to become an editing RNA target, leading to somatic hypermutation and protein diversity. Interestingly, LUAD patients harboring the RHOAiso2‐R176G mutation were associated with aberrant RHOA functions, cancer cell proliferation and migration, and poor clinical outcomes in transcriptome analysis. Mechanistically, RHOAiso2‐R176G mutation‐expressing LUAD cells potentiate RHOA‐guanosine triphosphate (GTP) activity to phosphorylate ROCK1/2 effectors and enhance cell proliferation and migration in vitro and increase tumor growth in xenograft and systemic metastasis models in vivo. Taken together, the RHOAiso2‐R176G mutation is a common somatic A‐to‐I edited mutation of the hypermutated RHOA isoform 2. It is an oncogenic and isoform‐specific theranostic target that activates RHOA‐GTP/p‐ROCK1/2 signaling to promote tumor progression.
A multi‐scale, multi‐region and attention mechanism‐based deep learning framework for prediction of grading in hepatocellular carcinoma
Authors: Wei Jingwei , Ji Qian , Gao Yu , Yang Xiaozhen , Guo Donghui , Gu Dongsheng , Yuan Chunwang , Tian Jie , Ding Dawei 
Published: December 2022

AbstractBackgroundHistopathological grading is a significant risk factor for postsurgical recurrence in hepatocellular carcinoma (HCC). Preoperative knowledge of histopathological grading could provide instructive guidance for individualized treatment decision‐making in HCC management.PurposeThis study aims to develop and validate a newly proposed deep learning model to predict histopathological grading in HCC with improved accuracy.MethodsIn this dual‐centre study, we retrospectively enrolled 384 HCC patients with complete clinical, pathological and radiological data. Aiming to synthesize radiological information derived from both tumour parenchyma and peritumoral microenvironment regions, a modelling strategy based on a multi‐scale and multi‐region dense connected convolutional neural network (MSMR‐DenseCNNs) was proposed to predict histopathological grading using preoperative contrast enhanced computed tomography (CT) images. Multi‐scale inputs were defined as three‐scale enlargement of an original minimum bounding box in width and height by given pixels, which correspondingly contained more peritumoral analysis areas with the enlargement. Multi‐region inputs were defined as three regions of interest (ROIs) including a squared ROI, a precisely delineated tumour ROI, and a peritumoral tissue ROI. The DenseCNN structure was designed to consist of a shallow feature extraction layer, dense block module, and transition and attention module. The proposed MSMR‐DenseCNN was pretrained by the ImageNet dataset to capture basic graphic characteristics from the images and was retrained by the collected retrospective CT images. The predictive ability of the MSMR‐DenseCNN models on triphasic images was compared with a conventional radiomics model, radiological model and clinical model.ResultsMSMR‐DenseCNN applied to the delayed phase (DP) achieved the highest area under the curve (AUC) of 0.867 in the validation cohort for grading prediction, outperforming those on the arterial phase (AP) and portal venous phase (PVP). Fusion of the results on triphasic images did not increase the predictive ability, which underscored the role of DP for grading prediction. Compared with a single‐scale and single‐region network, the DP‐phase based MSMR‐DenseCNN model remarkably raised sensitivity from 67.4% to 75.5% with comparable specificity of 78.6%. MSMR‐DenseCNN on DP defeated conventional radiomics, radiological and clinical models, where the AUCs were correspondingly 0.765, 0.695 and 0.612 in the validation cohort.ConclusionsThe MSMR‐DenseCNN modelling strategy increased the accuracy for preoperative prediction of grading in HCC, and enlightens similar radiological analysis pipelines in a variety of clinical scenarios in HCC management.
Exploring the experiences of frontline nurses during the first 6 months of the COVID‐19 pandemic: An integrated literature review
Authors: Jackson Megan R. , Porter Joanne E. , Mesagno Christopher 
Published: December 2022

AbstractAimThe aim of this study was to explore the experiences of frontline nurses caring for patients during the first 6 months of the COVID‐19 pandemic.DesignThe JBI manual for evidence synthesis and the PRISMA guidelines for reporting.Data sourcesCINAHL Complete, MEDLINE, PsycINFO (EBSCO) and Scopus (Elsevier).Review MethodsThe JBI Mixed Methods Data Extraction Tool following a Convergent Integrated Approach.ResultsNineteen studies were included in the review, comprising eight countries and 2525 frontline nurses. Six themes emerged encompassing frontline nurses' COVID‐19 experiences including emotional experiences, physical symptoms, ethical and moral challenges, professional impact, risk factors for negative emotional experiences and protective factors for future pandemic events.ConclusionFrontline nurses have faced numerous challenges during the COVID‐19 pandemic. Providing frontline nurses with the required resources and support to perform their roles in global healthcare crises allows for an empowered and resilient workforce ensuring nurses remain in their chosen profession.
Universal Cash Transfers and Labor Market Outcomes
Authors: Bibler Andrew , Guettabi Mouhcine , Reimer Matthew N. 
Published: December 2022

AbstractOne major criticism of Universal Basic Income is that unconditional cash transfers discourage recipients from working. Evidence to date has largely relied on targeted and/or conditional transfer programs. However, it is difficult to draw conclusions from such programs because universal transfers may induce a positive demand shock by distributing cash to a large portion of the population, which may in turn offset any negative labor supply responses. We estimate the causal effects of universal cash transfers on short‐run labor market activity by exploiting the timing and variation in size of a long‐running unconditional and universal transfer: Alaska's Permanent Fund Dividend. We find evidence of both a positive labor demand and negative labor supply response to the transfers. Small negative effects on the number of hours worked are found for women, especially those with young children. In contrast, we find an increase in the probability of employment for males in the months following the distribution. Altogether, a $1,000 increase in the per‐person disbursement leads to a 0.8 percent labor market contraction on an annual basis.
Poly(lactic acid) films reinforced with hybrid zinc oxide‐polyhedral oligomeric silsesquioxane nanoparticles: Morphological, mechanical, and antibacterial properties
Authors: Arshian Mahya , Estaji Sara , Tayouri Mohammad Iman , Mousavi Seyed Rasoul , Shojaei Shahrokh , Khonakdar Hossein Ali 
Published: December 2022

AbstractNanoparticles of zinc oxide (ZnO), polyhedral oligomeric silsesquioxane (POSS), and their hybrids were added to polylactic acid (PLA) using solution casting process at different weight percentages of the nanoparticles (1, 3, and 5 wt%) to form developed nanocomposites for food packaging applications. According to FTIR and XRD studies, both nanoparticles displayed fair chemical interactions with the PLA matrix. Furthermore, morphological investigations (SEM‐EDS) revealed that POSS, ZnO, and their hybrids were uniformly dispersed into the matrix despite some microaggregations at 5 wt% of nanoparticles, especially for those containing POSS. Moreover, adding 1 wt% of POSS to PLA increased its tensile strength and modulus by 20% and 149%, respectively, while decreasing the elongation‐at‐break by 54%. In addition, the tensile strength, tensile modulus, and elongation‐at‐break of PLA all increased by 7.5%, 28.7%, and 162%, respectively, with the addition of 1 wt% ZnO. Contact angle and surface free energy experiments demonstrated that after incorporating ZnO and POSS into the PLA matrix, the hydrophobicity of the samples increased, and POSS nanoparticles could minimize bacterial adherence effectively. In addition to the antibacterial properties of ZnO and POSS nanoparticles, increasing the concentration of hybrid nanoparticles up to 5 wt% expanded the bacteria's inhibitory zone, particularly in the case of Gram‐positive bacteria (Staphylococcus aureus). Finally, the hybrid nanocomposites exhibited both balanced mechanical characteristics and promising antibacterial activity compared with those with ZnO and POSS alone.
Lockdown in Chinese university dormitories: Significant increase in negative feelings and time perception distortion
Authors: Wang Juanjuan , Wang Chen , Gu Xiaoming , Sun Yi 
Published: December 2022

AbstractThe COVID‐19 pandemic has led to a global crisis resulting in lockdowns worldwide. Studies found that these could amplify negative affects predictive of disturbances in time perception. However, all existing studies on this topic concentrated on the general population during a lockdown at home. There was no research on university students living in dormitories. This study fills a gap in the literature by investigating the affective states and awareness of the passage of time of Chinese university students during an exceptional dormitory lockdown. Using a snowball sampling approach, this web‐based study recruited 225 volunteers (136 girls, 17−27 years old) during a dormitory lockdown. The online survey consisted of questions on demographic information, affective states (the Chinese version of the depression anxiety stress scales‐21 (DASS‐21) on depression, anxiety, and stress), and sense of the passage of time (the translated version of the assessment of passage of time over daily, weekly and yearly periods). The researchers shared the questionnaire link via the social media application WeChat. The study applied descriptive statistics and column charts to represent students' affective states and sense of the passage of time under extreme conditions and Pearson's correlation analysis to examine the impact of affective states on their sense of time. The study's results indicate that the dormitory lockdown caused a significant increase in all depression, anxiety, and stress categories of the DASS‐21, as well as a distortion of the sense of time passing. However, the direction of the distortion differed from that in previous studies. Moreover, a Pearson correlation analysis showed that none of the three negative affects could be associated with students' sense of time. The findings of this study indicate an increased vulnerability of students due to the lockdown. Their emotions were strongly affected, requiring particular interventions to preserve their mental well‐being. In addition, they showed a different distortion direction of time passage. Finally, they differed in the correlations between negative affect and the sense of passage of time compared to the general population in lockdown at home. This shows a need to investigate the impact of psychological states on students' routines and quality of life in emergencies. This study's scope should be expanded by incorporating additional factors pertaining to the passage of time and by integrating cross‐cultural comparisons.
Wogonin regulates colonocyte metabolism via PPARγ to inhibit Enterobacteriaceae against dextran sulfate sodium‐induced colitis in mice
Authors: Su Yulin , Liang Junjie , Zhang Meiling , Zhao Meng , Xie Xueqian , Wang Xiaojing , Pan Zengfeng , Huang Shaowei , Yan Rong , Wang Qing , Zhou Lian , Luo Xia 
Published: December 2022

AbstractTo investigate the potential effects and mechanism of wogonin on dextran sulfate sodium (DSS)‐induced colitis, 70 male mice were administered wogonin (12.5, 25, 50 mg·kg−1·d−1, i.g.) for 10 days, meanwhile, in order to induce colitis, the mice were free to drink 3% DSS for 6 days. We found that wogonin could obviously ameliorate DSS‐induced colitis, including preventing colon shortening and inhibiting pathological damage. In addition, wogonin could increase the expression of PPARγ, which not only restores intestinal epithelial hypoxia but also inhibits iNOS protein to reduce intestinal nitrite levels. All these effects facilitated a reduction in the abundance of Enterobacteriaceae in DSS‐induced colitis mice. Therefore, compared with the DSS group, the number of Enterobacteriaceae in the intestinal flora was significantly reduced after administration of wogonin or rosiglitazone by 16s rDNA technology. We also verified that wogonin could promote the expression of PPARγ mRNA and protein in Caco‐2 cells, and this effect disappeared when PPARγ signal was inhibited. In conclusion, our study suggested that wogonin can activate the PPARγ signal of the Intestinal epithelium to ameliorate the Intestinal inflammation caused by Enterobacteriaceae bacteria expansion.
Genomic imprinting in human placentation
Authors: Kobayashi Eri H. , Shibata Shun , Oike Akira , Kobayashi Norio , Hamada Hirotaka , Okae Hiroaki , Arima Takahiro 
Published: December 2022

AbstractBackgroundGenomic imprinting (GI) is a mammalian‐specific epigenetic phenomenon that has been implicated in the evolution of the placenta in mammals.MethodsEmbryo transfer procedures and trophoblast stem (TS) cells were used to re‐examine mouse placenta‐specific GI genes. For the analysis of human GI genes, cytotrophoblast cells isolated from human placental tissues were used. Using human TS cells, the biological roles of human GI genes were examined.Main findings(1) Many previously identified mouse GI genes were likely to be falsely identified due to contaminating maternal cells. (2) Human placenta‐specific GI genes were comprehensively determined, highlighting incomplete erasure of germline DNA methylation in the human placenta. (3) Human TS cells retained normal GI patterns. (4) Complete hydatidiform mole‐derived TS cells were characterized by aberrant GI and enhanced trophoblastic proliferation. The maternally expressed imprinted gene p57KIP2 may be responsible for the enhanced proliferation. (5) The primate‐specific microRNA cluster on chromosome 19, which is a placenta‐specific GI gene, is essential for self‐renewal and differentiation of human TS cells.ConclusionGenomic imprinting plays diverse and important roles in human placentation. Experimental analyses using TS cells suggest that the GI maintenance is necessary for normal placental development in humans.
Mass spectrometry: A key tool in anti‐doping
Authors: Dhurjad Pooja , Jaiswal Pooja , Gupta Kajal , Wanjari Parita , Sonti Rajesh 
Published: December 2022

AbstractPerformance enhancement drugs build muscle mass and increase stamina in athletes, making them a potential candidate for abuse in sports competitions. World Anti‐Doping Agency is an international organization responsible for developing standard guidelines, listing agents prohibited for usage in all athletic events, and taking stringent actions for any discrepancies. Sophisticated analytical instruments and statistical tools are regularly used for the chemical fingerprinting of doping agents in different biological matrices. There is an ever‐increasing demand for developing novel and sensitive mass spectrometry methods for detecting new substances of abuse. This review focuses on applying hyphenated chromatographic techniques, emphasizing advanced mass spectrometric methods to analyze different classes of prohibited substances listed by the World Anti‐Doping Agency.