bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance Analysis of Ionospheric TECmodelsoverthe Africanregion during the geomagnetic storm of March 2015
Authors: Jean de Dieu Nibigira , DEVANABOYINA VENKATA RATNAM , kondaveeti sivakrishna 
Published: July 2022

Abstract
This paper investigates the diurnal variations of modelled and observed Vertical Total Electron Content (VTEC) over the African region (40oN to + 40oS, 25oW to 65oE) obtained from ground-based Global Navigation Satellite System (GNSS) receivers. The investigations on ionospheric response during the super geomagnetic storm time (17 March 2015) is very crucial, especially over African low latitudes. Hence, the performance of ionospheric models has been evaluated in this paper. The VTEC predictability by regional/global ionospheric models (AfriTEC, IRI-2016, IRI-Plas 2017, GIM-CODE and Nequick-G) is evaluated by using root mean square error (RMSE) method and percentage deviation by comparing the GPS/GNSS-VTEC obtained from 10 IGS (International GNSS Service) stations with the modelled-VTEC values over African region. It is observed that peculiarity in VTEC values is evident during the superstorm sudden commencement when compared to the pre and post-storm periods. Northern hemisphere stations data showed/revealed a twin peak in the diurnal VTEC patterns. The enhanced VTEC values were observed over all the 10 IGS stations on the storm day than on other quiet days. Moreover, during the post-storm days (18–20 March 2015), these VTEC values decreased more than on quiet days over the IGS stations in southern hemisphere (MBAR, MAYG, HARB, SBOK). On the other hand, during the post-storm days (18–20 March 2015), the VTEC values remained high over the geomagnetic northern hemisphere (NOT1, SFER, MAS1, CPVG, NKLG). It is worth mentioning that three northern IGS stations (NOT1, SFER and MAS1) displayed a VTEC increase record of approximately 75–90%, which is due to the extension of Equatorial Ionization Anomaly (EIA) during the geomagnetic storm whereas the other northern stations at EIA trough region (CPVG, BJCO, NKLG) registered a VTEC increment of 7%, 26% and 25% respectively. Southern IGS stations registered an enhancement in VTEC of about 5%. The VTEC maps from AfriTEC, IRI-2016 and Nequick-G were able to predict the feature of Equatorial Ionospheric Anomaly (EIA) at around 200N/150S. The GPS-VTEC values at IGS stations located on the geomagnetic EIA crests (in both northern and southern hemispheres) and in the trough (equatorial stations) are higher than those of the IGS stations situated at midlatitudes. AfriTEC, which is a regional model, recorded lowest RMSE values over all the stations. The prediction results show that the regional model performance is better than the global ionospheric models (IRI-2016 and Nequick-G models) especially over EIA latitudes of African region.
DA-UNet: Deep Supervision and Atrous Inception-based U-Net Combining CRF for Automatic Liver Segmentation from CT
Authors: Lv Peiqing , Wang Jinke , Zhang Xiangyang , Shi Changfa 
Published: July 2022

Abstract
Due to low contrast and the blurred boundary between liver tissue and neighboring organs sharing similar intensity values, the problem of liver segmentation from CT images has not yet achieved satisfactory performance and remains a challenge. To alleviate these problems, we introduce deep supervision (DS) and atrous inception (AI) technologies with conditional random field (CRF) and propose three major improvements that are experimentally shown to have substantive and practical value. First, we replace the encoder's standard convolution with the residual block. Residual block can increase the depth of the network. Second, we provide AI module to connect the encoder and decoder. AI allows us to obtain multi-scale features. Third, we incorporate the DS mechanism into the decoder. This helps to make full use of information of the shallow layers. In addition, we employ the Tversky loss function to balance the segmented and non-segmented regions and perform the further refinement with a dense CRF. Finally, we extensively validate the proposed method on three public databases: LiTS17, 3DIRCADb, and SLiver07. Compared to the state-of-the-art (SOTA) methods, the proposed method achieved increased segmentation accuracy for the livers with low contrast and the fuzzy boundary between liver tissue and neighboring organs and is, therefore, more suited for automatic segmentation of these livers.
Tetramethylpyrazine Suppresses the Enhanced Ca2+ Sensitivity through Inhibiting the Expression of RhoA-ROCK in Artery of Simulated Weightlessness Rats
Authors: Wang Huiping , Yang Huajian , Kong Yong , Li Dan , Mou Rong , Zhao Zeyu , Li Yuanrui , Sun Yize , Cui Yanling , Gao Yunfang 
Published: July 2022

Abstract
Under weightlessness condition, the vasoconstriction will present the regional specific change. This study is to explore the role of myofilament Ca2+ sensitivity and its regulator RhoA-ROCK in these alterations, and the effect of tetramethylpyrazine on it. Female SD Rats tail suspending was used to simulate weightlessness. This study found that in weightless carotid artery, the vasoconstriction induced by PHE and KCl enhanced. The Ca2+ sensitivity, the protein expression of RhoA and ROCK II, and the phosphorylation level of MYPT1 and MLC increased. Tetramethylpyrazine administration for weightless rats could weaken the vasoconstriction, decrease the Ca2+ sensitivity, and reduce the above protein expressions and phosphorylation levels. Pretreatment with Y-27632 to inhibit the ROCK activity could decrease the vasoconstriction and the Ca2+ sensitivity in weightless rats, but have no effect in weightlessness plus tetramethylpyrazine administration rats. In weightless mesenteric artery, the vasoconstriction weakened. The Ca2+ sensitivity, the above protein expressions and phosphorylation levels decreased. Both tetramethylpyrazine administration and pretreatment with Y-27632 had no effects in weightlessness rats. These results suggest that the distinct alteration of myofilament Ca2+ sensitivity regulated by RhoA-ROCK possibly is the key factor resulting in the weightless vasoconstriction presenting different changes. Tetramethylpyrazine can inhibit the RhoA-ROCK protein expression to depress the enhanced weightless vasoconstriction in anterior part of body, but has no influence on the weakened vasoconstriction in posterior part of body. The present study clarifies the change of Ca2+ sensitivity and its mechanism in weightless artery, and broadens the application of tetramethylpyrazine in the treatment of vascular weightless dysfunction.
Construction of electrochemiluminescence aptasensor for acetamiprid detection using flower-liked SnO 2 nanocrystals encapsulated Ag3PO4 composite as luminophore
Authors: Su Chang , Dong Chunping , Jiang Ding , Shan Xueling , Chen ZhiDong 
Published: July 2022

Abstract
Acetamiprid is a kind of broad-spectrum pesticides that has been largely applied in agriculture and household pest control. In this paper, a simple, novel and highly sensitive electrochemiluminescence aptamer sensor (ECL aptasensor) based on Ag3PO4-NH2-SnO2 was proposed. The ECL signal was greatly amplified with the synergistic effect of Ag3PO4 and NH2-SnO2 (Ag3PO4-NH2-SnO2) which was dropped on a glass carbon electrode (GCE) and used as working electrode. We designed an “on–off” ECL sensing strategy for the sensitive and specific detection of acetamiprid. Under the optimal conditions, the linear range of the sensor for acetamiprid detection was 1.0 × 10− 11 ~ 1.0 × 10− 5 M, with a detection limit of 2.5 × 10− 12 M (S/N = 3). The method showed simple and fast operation, high sensitivity and selectivity, strong anti-interference ability and good stability. More importantly, the developed aptasensor exhibited excellent recognition ability towards residual acetamiprid in actual water samples, which might open promising avenues to develop new ECL systems for biological analysis and environmental water monitoring.
Successful allogenic bone marrow transplant post reduced-intensity conditioning chemotherapy in an adult patient with severe phenotype Glanzmann thrombasthenia: A case report
Authors: AlQurashi Wejdan Salem , AlJohani Naif I. 
Published: July 2022

Abstract
Background: In patients with severe Glanzmann thrombasthenia (GT), a genetic platelet surface receptor disorder of glycoprotein IIb/IIIa (integrin αIIbβ3) that results in prolonged and chronic bleeding, hematopoietic stem cell transplantation (HSCT) is the only curative treatment option. Unfortunately, approximately 30% of all patients undergoing HSCT die from this toxic procedure, owing to the associated risk of infection or chronic graft-versus-host disease, with adults at a higher risk.Case presentation: Herein, we describe the case of a 21-year-old woman from Saudi Arabia who had symptomatic GT for the past 15 years and underwent HSCT following a modified reduced-intensity conditioning (RIC) protocol. This is the fifth reported case of the use of HSCT for treating an adult patient with GT in which RIC HSCT has resulted in long term sustained chimerism with minimal toxicity. The patient was genetically confirmed to have GT since childhood. Owing to recurrent severe bleeding events, the patient had received multiple blood and platelet transfusions and hormonal therapy affecting her quality of life. In September 2019, the patient underwent allogeneic stem cell transplantation from her HLA-matched healthy brother, who was genetically negative for GT. A reduced-intensity conditioning chemotherapy regimen of anti-thymocyte globulin, fludarabine/busulfan (9.6 mg/kg), and a dose of 2.96 × 106 CD34+ cells were administered. The patient experienced moderate mucositis with associated oral mucosal bleeding during HSCT, which was managed. Low cytomegalovirus viremia occurred 25 days after transplant and resolved with treatment. Neutrophil engraftment occurred on day +23, and platelet engraftment on day +27. A platelet aggregation test repeated after 1 year and 10 months was negative. At 24 months following HSCT, the patient was well and graft versus host disease-free, had no bleeding tendency, and discontinued all immunosuppressant therapy.Conclusion: HSCT in adults with severe phenotype GT is possible if indicated, but careful consideration of different aspects of care should be considered and strictly followed for a successful outcome. Future research through more case reports or series utilizing similar reduced intensity conditioning HSCT is needed to fill the knowledge gap given the rarity of indication and the new protocol.
Psychological Impact Assessment of Covid-19 on Migrants in Jammu City and Peripheral Area, Jammu and Kashmir, India
Authors: Singh Harpreet , sharma sanjana 
Published: July 2022

Abstract
Introduction: In Indian economy, the internal migrants are the major role player in the informal sector. During Covid-19 pandemic and subsequent lockdown millions of internal migrants were severely affected. Migrants are more prone to social, economic, and psychological issues. It is quite relevant to know the impact of Covid-19 on the psychological health of migrants. The fear of starvation, fear of not being able to meet their family members, fear of getting infected, fear of death, loss of wages were the main psychological problems they faced. This paper focuses on the psychological assessment of impact of Covid-19 on migrants in the Jammu city and its peripheral areas, Union Territory Jammu & Kashmir, India. Methodology: Data is collected by personal interview method with the help of questionnaire. For analyzing the psychological impacts of Covid-19 on migrants, we used Patient Health Questionnaire-2 and Patient Health Questionnaire-9. 100 persons are surveyed during the study. Results: 69 percent respondent reflected that they had fear of death during pandemic period. Most of the respondent said that they were feeling nervous, helpless for not being able to attend the health needs of family even they were not able to concentrate properly and had little interest in doing things.Conclusion: the outcome of the study shows that more than half of the respondents are dealing with the psychological issues, thus along with looking at the physical needs there is need of taking care of mental health is also especially in the time of the pandemic.
Pipsqueak family genes dan/danr antagonize nuclear Pros and prevent premature decommission of neural stem cells in Drosophila third instar larval brains
Authors: An Huanping , Yu Yue , Ren Xuming , Zeng Minghua , Bai Yu , Liu Tao , Zheng Huimei , Sang Rong , Zhang Fan , Cai Yu , Xi Yongmei 
Published: July 2022

Abstract
Background
Neural stem cell fate is regulated for the systematic production of a precise quantity of neurons during the development of the central nervous system.
Results
Here we found that two Drosophila pipsqueak family genes, distal antenna (dan) and distal antenna-related (danr), promote the proliferation of neural stem cells, termed neuroblasts (NBs), in third instar larval brains. In the absence of Dan and Danr (Dan/Danr), the NBs produce fewer daughter cells with smaller MARCM clone (lineage) sizes. The larval brain NBs in dan/danr clones show premature accumulation of nuclear Prospero (Pros), which only appears in the wildtype (wt) terminating NBs at early pupal stage. The premature nuclear Pros leads to NBs cell cycle defects and NB marker loss. Removal of Pros from dan/danr MARCM clones prevents lineage size shrinkage and rescues the loss of NB marker expression. Our data show that the appearance of nuclear Pros is behind the downregulation of Dan/Danr in the wt terminating NBs. We demonstrate that Dan/Danr and nuclear Pros are mutually exclusive in NBs. In addition, Dan/Danr are partially required for the late temporal regulator, Grainyhead (Grh), in third instar larval brain NBs.
Conclusion
Together our study uncovers the novel function of Dan/Danr in NB cell fate maintenance. Dan/Danr antagonize nuclear Pros, preventing NBs from premature decommission in Drosophila third instar larval brains.
Effect of nitrate on power generation and nitrogen removal and bacterial community in Microbial fuel cell (MFC)
Authors: Wang Luyu , Xue Hongbin , Gong Lei , Jia Qinwei , Ma Xiaofan , Zhou Jun , Zhao Yuhang , Wang Jin 
Published: July 2022

Abstract
Microbial fuel cell (MFC) technology has the function of decarburization and denitrification and is considered advantageous in treating wastewater with a low carbon-nitrogen ratio. In the current study, MFC treats different concentrations of nitrate nitrogen. And compared with abiotic electrode treatment and microbial treatment only, MFC showed obvious treatment advantages. The best treatment effect was recorded when the nitrate-nitrogen (NO3-N) concentration was 414 mg/L. The running of the MFC system showed that it produced a stable output voltage up to 0.17 V within 160 h; And showed a power density up to 40.18 mW/m2, which was 1.58 times of M1 (138 mg/L, 25.49 mW/m2), 4.48 times of non-biological electrode; The removal rate of NO3-N was 97.48%, but the lack of anode electron supply resulted in the incomplete reduction of nitrate nitrogen and accumulation of nitrite-nitrogen (188.83 mg/L). Nitrate-nitrogen concentration in the study had no significant impact on microbial population diversity. However, the relative abundance of Proteobacteria increased from 47.3% to 65.4% when NO3-N concentration increased from 138 to 414 mg/L. Results from this study are promising for a theoretical basis for microbial cathode denitrification.
Establishment of animal models for NASH and utilization for mining associated gut microbiota
Authors: Sheen Lee-Yan , Panyod Suraphan , Wu Wei-Kai , Hsieh Ya-Chi , Tseng Yea-Jing , Peng Sin-Yi , Chen Rou-An , Huang Huai-Syuan , Chen Yi-Hsun , Shen Ting-Chin David , Ho Chi-Tang , Liu Chun-Jen , Chuang Hsiao-Li , Huang Chi-Chang , Wu Ming-Shiang 
Published: July 2022

Abstract
A high-fat diet (HFD) animal model has an indispensable role in the research of gut-liver axis by generating an imbalance of gut microbiota and intestinal leakage, resulting in translocation of harmful bacterial metabolites and lipopolysaccharides (LPSs) to the liver, triggering immune responses, and stimulating non-alcoholic steatohepatitis (NASH). Replacing trans-fat with palm oil in HFDs is an emerging animal model with human translatability in terms of liver biopsy phenotype and transcriptome changes. We developed the NASH and fibrosis mouse model using a palm oil-containing high-fat diet (P-HFD) combined with LPS to simulate gut dysbiosis and endotoxemia and examined the role of carbon tetrachloride (CCl4) on the gut-liver axis. The mice were fed P-HFD with or without intraperitoneal LPS/CCl4 injections. NASH/fibrosis progression and gut microbiota shift were examined at 4, 8, 12, 16, 20, and 24 weeks (n = 192). The P-HFD animal model showed obesogenic and metabolic changes, hepatomegaly, NASH phenotype, increased gut dysbiosis, changes in gut microbiota and its function, reduced beneficial bacteria, increased pathogenic related microbiome, intestinal leakage, and endotoxemia. P-HFD with LPS supplementation resulted in comparable NASH phenotypes compared with the P-HFD group, but worsened the degree of intestinal microbiota dysbiosis by increasing the occurrence of pathogenic bacteria and reducing beneficial microbiota. P-HFD with CCl4 resulted in a liver fibrosis phenotype and produced a more severe gut microbiota shift. These mouse models could be informative for a researcher focused on the gut-liver axis and could be used as valuable tool for preclinical drug testing.
A Randomised Trial of Ultrasonographic Assessment of Gastric Emptying Following Water Ingestion in Healthy Volunteers
Authors: Headdon William , Ballisat Ben , Thompson Christopher , Klepsch Peter , Leary Sam , Hunt Linda , Ness Andy , Atkinson Charlotte 
Published: July 2022

Abstract
Background:
Current guidance on pre-operative fasting for clear fluids to reduce aspiration risk may result in unnecessary dehydration and more liberal fluid regimes are being advocated.
Methods:
This randomised single blinded crossover study used an established ultrasound protocol to measure the gastric volume of 15 healthy volunteers following the 2011 European Society of Anaesthesia (ESA) ‘Perioperative Fasting in Adults and Children’ guidelines (nil-by-mouth two hours before induction of anaesthesia) and the 2005 Scandinavian guidelines (150 mL water with medication up to 1 hour before induction of anaesthesia).
Results:
We observed that gastric volumes increased following ingestion of water in all individuals compared to their fasted states. Water left the stomach within 30 minutes following ingestion in ten individuals, and within an hour in three individuals (gastric volume < 1.5 ml kg− 1). A reduction in thirst was observed in the group allowed to drink water, whereas hunger and anxiety scores were unaffected.
Multiplexed digital spatial protein profiling reveals a unique protein signature for advanced liver fibrosis
Authors: Lee Jaejun , Kim Chang Min , Cha Jung Hoon , Park Jin Young , Yu Yun Suk , Wang Hee Jung , Sung Pil Soo , Jung Eun Sun , Bae Si Hyun 
Published: July 2022

Abstract
Background and Aims:
Intrahepatic mononuclear phagocytes (MPs) are critical for the initiation and progression of liver fibrosis. In this study, using multiplexed digital spatial protein profiling, we aimed to derive a unique protein signature predicting advanced liver fibrosis.
Methods
Snap-frozen liver tissues from various chronic liver diseases were subjected to spatially defined protein-based multiplexed profiling (Nanostring GeoMX™). Single-cell RNA sequencing analysis was performed using Gene Expression Omnibus (GEO) datasets from normal and cirrhotic livers.
Results
Sixty-four portal regions of interest (ROIs) were selected for the spatial profiling. Combined analysis of single-cell RNA sequencing data from GEO datasets (GSE136103) and spatially-defined, protein-based multiplexed profiling revealed that most proteins upregulated in F0–F2 livers in portal CD68+ cells were specifically marked in tissue monocytes whereas proteins upregulated in F3 and F4 livers were marked in SAMacs and tissue monocytes. Internal validation using mRNA expression data with the same cohort tissues demonstrated that mRNA levels TREM2, CD9, and CD68 are significantly higher in livers with advanced fibrosis. Using the results from the CD68+ area, a highly sensitive and specific immune-related protein signature (CD68, HLA-DR, OX40L, phospho-c-RAF, STING, and TIM3) was developed to predict advanced (F3 and F4) fibrosis.
Conclusions
In patients with advanced liver fibrosis, portal MPs consist heterogeneous populations composed of SAMacs, Kupffer cells, and tissue monocytes. Using digital spatial protein profiling, the protein signature with high accuracy in predicting advanced fibrosis was developed.
Improving wheat yield and water use efficiency by optimizing irrigations in northern China
Authors: Zhang Xin , Xue Jiaxin , Zhang Jiangwei , Zhang Jianheng , Wang Guiyan 
Published: July 2022

Abstract
Recently, to achieve the goal of increasing both crop yield and water/nitrogen use efficiency with a better irrigation regime is a major challenge in semi-arid areas. In this study, we presented a two seasonal-field experiment that considers irrigation regimes, i.e., no irrigation (W0), irrigated in jointing (W1), both in jointing and flowering (W2) after the re-greening, and varieties (S086; J22) to compare the response of the sensitivity of wheat leaf physiological indicators, yield, water/N use efficiency and soil water consumption to irrigation regimes. The results showed that the WUE, IWUE and soil water-holding consumption (SWC) decreased with the increase in amount of irrigation. Additionally, 45.5% of the excessive irrigation water input did not promote wheat yield (W1 vs. W2). The degree of SWC in the 0–120 cm soil layer was highly related to wheat growth. S086 was beneficial for the usage of SWC under a low amount of irrigation. As well, irrigation positively affected the activities of superoxide dismutase (SOD) and catalase (CAT) in the flag leaf (P<0.05) during crop yield production. A decrease of irrigation helped to increase the concentrations of SS and Pro and decrease of amount of MDA for S086. Thus, a high yield of S086 was found under deficit irrigation (W1, a 31.3% reduction of irrigation water than that of W2). Thus, our studies suggested that one irrigation event in jointing stage for the S086 variety was essential to meet the win-win goal of high crop yield and water use efficiency with low groundwater consumption.
Naked-eye optical recognition of ammonia vapor and melamine in water using a fluorophore appended polymer matrix
Authors: Bhuin Shouvik , Chakravarty Manab 
Published: July 2022

Abstract
The generation of solid-state emitters is a challenge due to the intrinsic aggregation-caused quenching feature of the fluorophores. A conformationally twisted pyridyl π-conjugate as a solid-state emitter is appended with well-known and inexpensive poly(methylmethacrylate) [PMMA] to afford a handy, portable, and reusable solid-state emitting polymer matrix. Entrapment of the probe is noticed through non-covalent interactions, resulting in a green-emitting platform. It quickly accepts a proton upon acid vapor exposure and switches emission from green to red with a significant 107 nm redshift. This shift is reversible with red to green emissions while exposed to base vapor. Thus, polymer-blended, homogeneous red-emitting pyridyl salt is employed as potential material to detect various basic vapor optically. Among different bases, naked-eye detection of essential analytes such as ammonia vapor and melamine shows potential demands. Hence, we have established an easy detection of ammonia vapour and aqueous melamine as low as 2.5 and 0.126 ppm, respectively, using this solid-state emitter that displays an emission color change with an enhancement of emission intensity even in aqueous solution.