bluuebunny/crossref_metadata_2025_split huggingface dataset:
Probabilistic model checking of cancer metabolism
Authors: Friedenberg Meir D. , Lita Adrian , Gilbert Mark R. , Larion Mioara , Celiku Orieta 
Published: November 2022

AbstractCancer cell metabolism is often deregulated as a result of adaption to meeting energy and biosynthesis demands of rapid growth or direct mutation of key metabolic enzymes. Better understanding of such deregulation can provide new insights on targetable vulnerabilities, but is complicated by the difficulty in probing cell metabolism at different levels of resolution and under different experimental conditions. We construct computational models of glucose and glutamine metabolism with focus on the effect of IDH1/2-mutations in cancer using a combination of experimental metabolic flux data and patient-derived gene expression data. Our models demonstrate the potential of computational exploration to reveal biologic behavior: they show that an exogenously-mutated IDH1 experimental model utilizes glutamine as an alternative carbon source for lactate production under hypoxia, but does not fully-recapitulate the patient phenotype under normoxia. We also demonstrate the utility of using gene expression data as a proxy for relative differences in metabolic activity. We use the approach of probabilistic model checking and the freely-available Probabilistic Symbolic Model Checker to construct and reason about model behavior.
Increased interferon-γ levels and risk of severe malaria: a meta-analysis
Authors: Mahittikorn Aongart , Mala Wanida , Masangkay Frederick Ramirez , Kotepui Kwuntida Uthaisar , Wilairatana Polrat , Kotepui Manas 
Published: November 2022

AbstractInterferon (IFN)-γ contributes to the pathogenesis of severe malaria; however, its mechanism remains unclear. Herein, differences in IFN-γ levels between patients with severe and uncomplicated malaria were evaluated using qualitative and quantitative (meta-analysis) approaches. The systematic review protocol was registered at PROSPERO (ID: CRD42022315213). The searches for relevant studies were performed in five databases, including PubMed, Scopus, Embase, MEDLINE and Web of Science, between 1 January and 10 July 2022. A meta-analysis was conducted to pool the mean difference (MD) of IFN-γ levels between patients with severe malaria and those with uncomplicated malaria using a random-effects model (DerSimonian and Laird method). Overall, qualitative synthesis indicated that most studies (14, 58.3%) reported no statistically significant difference in IFN-γ levels between patients with severe malaria and those with uncomplicated malaria. Meanwhile, remaining studies (9, 37.5%) reported that IFN-γ levels were significantly higher in patients with severe malaria than those in patients with uncomplicated malaria. Only one study (4.17%) reported that IFN-γ levels were significantly lower in patients with severe malaria than those in patients with uncomplicated malaria. The meta-analysis results indicated that patients with severe malaria had higher mean IFN-γ levels than those with uncomplicated malaria (p < 0.001, MD: 13.63 pg/mL, 95% confidence interval: 6.98–20.29 pg/mL, I2: 99.02%, 14 studies/15 study sites, 652 severe cases/1096 uncomplicated cases). In summary, patients with severe malaria exhibited higher IFN-γ levels than those with uncomplicated malaria, although the heterogeneity of the outcomes is yet to be elucidated. To confirm whether alteration in IFN-γ levels of patients with malaria may indicate disease severity and/or poor prognosis, further studies are warranted.
Children’s perception of interpersonal coordination during joint painting
Authors: Abraham Rotem , Grinspun Noemí , Rabinowitch Tal-Chen 
Published: November 2022

AbstractInterpersonal coordination is important for many joint activities. A special case of interpersonal coordination is synchronization, which is required for the performance of many activities, but is also associated with diverse positive social and emotional attributes. The extent to which these effects are due to the reliance on synchrony for task performance or to its specific rhythmic characteristics, is not clear. To address these questions, we considered a more general form of interpersonal coordination, implemented during joint artmaking. This is a non-typical context for interpersonal coordination, not required for task success, and smoother and more loosely-structured than more standard forms of synchronous coordination. Therefore, comparing interpersonal coordination with non-coordination during shared painting, could help reveal general social-emotional reactions to coordination. To gain a more ‘naïve’ perspective we focused on children, and staged coordinated and non-coordinated art interactions between an adult and a child, asking child observers to judge various variables reflecting the perceived bond between the painters. We found an overall stronger perceived bond for the coordination condition. These results demonstrate that even a non-typical form of interpersonal coordination could be attributed with positive social and emotional qualities, a capacity revealed already in childhood, with possible implications for development.
Prevalence and associations of dry eye disease and meibomian gland dysfunction in the ural eye and medical study
Authors: Bikbov Mukharram M. , Gilmanshin Timur R. , Zainullin Rinat M. , Kazakbaeva Gyulli M. , Iakupova Ellina M. , Fakhretdinova Albina A. , Tuliakova Azaliia M. , Panda-Jonas Songhomitra , Rusakova Iuliia A. , Nuriev Ildar F. , Zaynetdinov Artur F. , Miniazeva Liana A. , Jonas Jost B. 
Published: November 2022

AbstractTo assess the prevalence of dry eye disease (DED) and Meibomian gland dysfunction (MGD) in a population in Russia. The population-based Ural Eye and Medical Study was conducted in an urban and rural region in Bashkortostan/Russia and included 5899 (80.5%) out of 7328 eligible persons, aged 40 + years. DED and MGD were assessed by Schirmer´s test, slit-lamp based examination of the Meibomian glands, and an interview with DED-related questions. The study included 5153 (87.4%) individuals with DED and MGD assessments (mean age: 58.5 ± 10.5 years). The mean Schirmer´s test result was 11.8 ± 6.8 mm and 12.5 ± 7.1 mm for the right/left eye, with a result of ≤ 5 mm measured in 1098 (21.3%; 95% confidence intervals (CI) 20.2, 22.4) of the worse eyes. The mean subjective dry eye symptoms score was 1.37 ± 1.82. MGD grade 1 (telangiectasia at the lid margin), 2, 3, 4 or any grade in the worse eye was diagnosed in 901 (21.1%), 1161 (27.1%), 158 (3.7%), 32 (0.7%), and 2252 (52.6%; 95% CI 51.1, 54.1) eyes respectively. The prevalence of DED diagnosis #1, #2 and #3 (Schirmer´s test ≤ 5 mm, and dry eye score ≥ 1, ≥ 2, and ≥ 3, respectively), #4 (dry eye score ≥ 1, Schirmer test ≤ 5 mm, MGD grade 1 +), and #5 (dry eye score ≥ 1, Schirmer test ≤ 5 mm, MGD grade 2 +) were 598/5142 (11.6%), 426/5153 (8.3%), 273/5142 (5.3%), 335/5142 (6.5%), and 186/5142 (3.6%), respectively. Mean depression score as applied was 5.1 ± 3.8. Higher DED prevalence (definition #4) was associated (multivariable analysis) with female sex (odds ratio (OR) 1.71; 95% CI 1.31, 2.22; P < 0.001), higher depression score (OR 1.04; 95% CI 101, 1.07; P = 0.009), and higher prevalence of thyroid disease history (OR 1.63; 95% CI 1.19, 2.24; P = 0.006). DED and MGD were common in this rural and urban population, and their prevalence was associated with female sex, thyroid disease, and depression.
Genomic control of inflammation in experimental atopic dermatitis
Authors: Liu Yan , Zienkiewicz Jozef , Qiao Huan , Gibson-Corley Katherine N. , Boyd Kelli L. , Veach Ruth Ann , Hawiger Jacek 
Published: November 2022

AbstractAtopic Dermatitis (AD) or eczema, a recurrent allergic inflammation of the skin, afflicts 10–20% of children and 5% adults of all racial and ethnic groups globally. We report a new topical treatment of AD by a Nuclear Transport Checkpoint Inhibitor (NTCI), which targets two nuclear transport shuttles, importin α5 and importin β1. In the preclinical model of AD, induced by the active vitamin D3analog MC903 (calcipotriol), NTCI suppressed the expression of keratinocyte-derived cytokine, Thymic Stromal Lymphopoietin (TSLP), the key gene in AD development. Moreover, the genes encoding mediators of TH2response, IL-4 and its receptor IL-4Rα were also silenced together with the genes encoding cytokines IL-1β, IL-6, IL-13, IL-23α, IL-33, IFN-γ, GM-CSF, VEGF A, the chemokines RANTES and IL-8, and intracellular signal transducers COX-2 and iNOS. Consequently, NTCI suppressed skin infiltration by inflammatory cells (eosinophils, macrophages, and CD4 + T lymphocytes), and reduced MC903-evoked proliferation of Ki-67-positive cells. Thus, we highlight the mechanism of action and the potential utility of topical NTCI for treatment of AD undergoing Phase 1/2 clinical trial (AMTX-100 CF, NCT04313400).
Influential factors of saliva microbiota composition
Authors: Wells Philippa M. , Sprockett Daniel D. , Bowyer Ruth C. E. , Kurushima Yuko , Relman David A. , Williams Frances M. K. , Steves Claire J. 
Published: November 2022

AbstractThe oral microbiota is emerging as an influential factor of host physiology and disease state. Factors influencing oral microbiota composition have not been well characterised. In particular, there is a lack of population-based studies. We undertook a large hypothesis-free study of the saliva microbiota, considering potential influential factors of host health (frailty; diet; periodontal disease), demographics (age; sex; BMI) and sample processing (storage time), in a sample (n = 679) of the TwinsUK cohort of adult twins. Alpha and beta diversity of the saliva microbiota was associated most strongly with frailty (alpha diversity: β = −0.16, Q = 0.003, Observed; β = −0.16, Q = 0.002, Shannon; β = −0.16, Q = 0.003, Simpson; Beta diversity: Q = 0.002, Bray Curtis dissimilarity) and age (alpha diversity: β = 0.15, Q = 0.006, Shannon; β = 0.12, Q = 0.003, Simpson; beta diversity: Q = 0.002, Bray Curtis dissimilarity; Q = 0.032, Weighted UniFrac) in multivariate models including age, frailty, sex, BMI, frailty and diet, and adjustment for multiple testing. Those with a more advanced age were more likely to be dissimilar in the saliva microbiota composition than younger participants (P = 5.125e−06, ANOVA). In subsample analyses, including consideration of periodontal disease (total n = 138, periodontal disease n = 66), the association with frailty remained for alpha diversity (Q = 0.002, Observed ASVs; Q = 0.04 Shannon Index), but not beta diversity, whilst age was not demonstrated to associate with alpha or beta diversity in this subsample, potentially due to insufficient statistical power. Length of time that samples were stored prior to sequencing was associated with beta diversity (Q = 0.002, Bray Curtis dissimilarity). Six bacterial taxa were associated with age after adjustment for frailty and diet. Of the factors studied, frailty and age emerged as the most influential with regards to saliva microbiota composition. Whilst age and frailty are correlates, the associations were independent of each other, giving precedence to both biological and chronological ageing as processes of potential importance when considering saliva microbiota composition.
An educational intervention based on family-centered empowerment model to modify high-risk behaviors of brucellosis via mother education
Authors: Shojaei Maryam Sadat , Tavakoly Sany Seyedeh Belin , Ghavami Vahid , Tehrani Hadi 
Published: November 2022

AbstractThe relative contribution of a theory-based intervention to modify high-risk behaviors in training programs is a major priority that remains an open question. Here, we tested whether the family-centered empowerment model used in the educational intervention was effective to modify high-risk behaviors of brucellosis via mother education. A quasi experimental study was conducted on 200 women presenting to healthcare practices in rural areas of Torbat-e Jam, Iran, from April 2020 to February 2021. Four rural areas were randomly assigned to the control and intervention groups. The intervention group received the training program, which included four 2-h sessions and consulting support via social network and messaging service. The control group did not receive any training. SPSS16was implemented to test multiple statistical analyses. Our finding showed in the intervention group compared with the control group, knowledge, attitude, self-efficacy, self-esteem, and behavior outcomes were significantly changed (P < 0.001) across time during baseline through follow-up. Likewise, there are no differences (P > 0.05) in the change in construct of the family-centered empowerment model and risk behaviors in the control group from baseline to follow-up. Intervention based on a family-centered empowerment model is possible and very acceptable to modify high-risk behaviors of brucellosis by increasing an individual’s knowledge, changing attitude, and promoting self-efficacy and self-esteem.Trial registration: Iranian Registry of Clinical Trials (IRCT), IRCT20160619028529N12. Registration date: 24/03/2020.
Design, fabrication and application of self-spiraling pattern-driven 4D-printed actuator
Authors: Zeng Siyuan , Gao Yicong , Qiu Hao , Xu Junjun , Tan Jianrong 
Published: November 2022

AbstractSelf-spiraling actuators are widely found in nature and have high research and actuator-application value in self-lock and self-assembly. Four-dimensional (4D) printing is a new generation additive manufacturing of smart materials and has shown great potential for the fabrication of multi-functional and customized structures. The microarchitecture design of a bilayer actuator could bring flexible and diversified self-spiraling behaviors and more possibilities for practical application by combing 4D printing. This work investigates the stimuli effects of fiber patterns and fabrication parameters on self-spiraling behaviors of the bilayer actuator via both experimental and theoretical methods. This work may potentially provide pattern design guidance for 4D-printed self-spiraling actuators to meet different application requirements.
Interactions between folate intake and genetic predictors of gene expression levels associated with colorectal cancer risk
Authors: Haas Cameron B. , Su Yu-Ru , Petersen Paneen , Wang Xiaoliang , Bien Stephanie A. , Lin Yi , Albanes Demetrius , Weinstein Stephanie J. , Jenkins Mark A. , Figueiredo Jane C. , Newcomb Polly A. , Casey Graham , Le Marchand Loic , Campbell Peter T. , Moreno Victor , Potter John D. , Sakoda Lori C. , Slattery Martha L. , Chan Andrew T. , Li Li , Giles Graham G. , Milne Roger L. , Gruber Stephen B. , Rennert Gad , Woods Michael O. , Gallinger Steven J. , Berndt Sonja , Hayes Richard B. , Huang Wen-Yi , Wolk Alicja , White Emily , Nan Hongmei , Nassir Rami , Lindor Noralane M. , Lewinger Juan P. , Kim Andre E. , Conti David , Gauderman W. James , Buchanan Daniel D. , Peters Ulrike , Hsu Li 
Published: November 2022

AbstractObservational studies have shown higher folate consumption to be associated with lower risk of colorectal cancer (CRC). Understanding whether and how genetic risk factors interact with folate could further elucidate the underlying mechanism. Aggregating functionally relevant genetic variants in set-based variant testing has higher power to detect gene–environment (G × E) interactions and may provide information on the underlying biological pathway. We investigated interactions between folate consumption and predicted gene expression on colorectal cancer risk across the genome. We used variant weights from the PrediXcan models of colon tissue-specific gene expression as a priori variant information for a set-based G × E approach. We harmonized total folate intake (mcg/day) based on dietary intake and supplemental use across cohort and case–control studies and calculated sex and study specific quantiles. Analyses were performed using a mixed effects score tests for interactions between folate and genetically predicted expression of 4839 genes with available genetically predicted expression. We pooled results across 23 studies for a total of 13,498 cases with colorectal tumors and 13,918 controls of European ancestry. We used a false discovery rate of 0.2 to identify genes with suggestive evidence of an interaction. We found suggestive evidence of interaction with folate intake on CRC risk for genes including glutathione S-Transferase Alpha 1 (GSTA1; p = 4.3E−4), Tonsuko Like, DNA Repair Protein (TONSL; p = 4.3E−4), and Aspartylglucosaminidase (AGA: p = 4.5E−4). We identified three genes involved in preventing or repairing DNA damage that may interact with folate consumption to alter CRC risk. Glutathione is an antioxidant, preventing cellular damage and is a downstream metabolite of homocysteine and metabolized by GSTA1. TONSL is part of a complex that functions in the recovery of double strand breaks and AGA plays a role in lysosomal breakdown of glycoprotein.
Binary THz modulator based on silicon Schottky-metasurface
Authors: Ahadi Saeedeh , Neshat Mohammad , Moravvej-Farshi Mohammad Kazem 
Published: November 2022

AbstractWe propose a metasurface THz modulator based on split-ring resonators (SRRs) formed by four interconnected horizontal Si–Au Schottky diodes. The equivalent junction capacitance of each SRR in the proposed modulator is much smaller than that of the previously reported metasurface counterparts with vertical Schottky junctions, leading to a higher modulation speed. To modulate a THz incident signal by the proposed metasurface, we vary the bias voltage externally applied to the Schottky junctions. Applying a reverse bias of VA =  − 5 V to the Au gate, two LC resonances at 0.48 THz, and 0.95 THz are excited in the metasurface. Switching the applied voltage to VA =  + 0.49 V, we diminish the oscillator strengths of the LC resonances, creating one dipole resonance at 0.73 THz in the transmission spectrum of the metasurface modulator. The modulation depths at these resonances are more than 45%, reaching 87% at 0.95 THz. The phase modulation for this THz modulator is about 1.12 rad at 0.86 THz. Furthermore, due to the particular design of the meta-atoms, the modulation speed of this device is estimated up to approximately several hundred GHz, which makes this device an appropriate candidate for high-speed applications in wireless communications systems based on external modulators.
Investigating the effects of low intensity visible light on human keratinocytes using a customized LED exposure system
Authors: Sutterby Emily , Chheang Chanly , Thurgood Peter , Khoshmanesh Khashayar , Baratchi Sara , Pirogova Elena 
Published: November 2022

AbstractPhotobiomodulation (PBM) refers to the use of light to modulate cellular processes, and has demonstrated utility in improving wound healing outcomes, and reducing pain and inflammation. Despite the potential benefits of PBM, the precise molecular mechanisms through which it influences cell behavior are not yet well understood. Inconsistent reporting of key light parameters has created uncertainty around optimal exposure profiles. In addition, very low intensities of light, < 0.1 J/cm2, have not been thoroughly examined for their use in PBM. Here, we present a custom-made compact, and modular LED-based exposure system for studying the effects of very low-intensity visible light (cell proliferation, migration, ROS production, and mitochondrial membrane potential) of three different wavelengths in a parallel manner. The device allows for six repeats of three different exposure conditions plus a non-irradiated control on a single 24-well plate. The immortalised human keratinocyte cell line, HaCaT, was selected as a major cellular component of the skin epidermal barrier. Furthermore, an in vitro wound model was developed by allowing the HaCaT to form a confluent monolayer, then scratching the cells with a pipette tip to form a wound. Cells were exposed to yellow (585 nm, 0.09 mW, ~ 3.7 mJ/cm2), orange (610 nm, 0.8 mW, ~ 31 mJ/cm2), and red (660 nm, 0.8 mW, ~ 31 mJ/cm2) light for 10 min. 48 h post-irradiation, immunohistochemistry was performed to evaluate cell viability, proliferation, ROS production, and mitochondrial membrane potential. The results demonstrate increased proliferation and decreased scratch area for all exposure conditions, however only red light increased the mitochondrial activity. Oxidative stress levels did not increase for any of the exposures. The present exposure system provides opportunities to better understand the complex cellular mechanisms driven by the irradiation of skin cells with visible light.
Simulation study on dynamic characteristics of gas diffusion in coal under nitrogen injection
Authors: Fang Xinliang 
Published: November 2022

AbstractTo reveal the mechanism of desorption of methane in coal seams by inert gas N2, the desorption behavior of CH4 after N2 injection was studied by using Giant Canonical ensemble Monte Carlo (GCMC) and Molecular Dynamics (MD) methods with wiser bituminous coal as the research object. The results show that the adsorption isotherms of CH4 and N2 in the molecular structure model of bituminous coal are in good agreement with the Langmuir adsorption isotherm model. The adsorption capacity of the two gases in the bituminous coal structure model is CH4 > N2. The higher the N2 injection pressure, the higher the temperature, and the more methane desorption. N2 can replace some adsorbed CH4 through competitive adsorption with CH4. Compared with injecting high-temperature nitrogen to desorb methane in coal seams, in high-pressure nitrogen, the diffusion effect of CH4 flowing in coal is more significant. The higher the nitrogen injection pressure, the better the effect of N2 promoting CH4 desorption. The relative concentration of CH4 in the vacuum layer gradually increases with the increase of water content. This indicates that the water in coal promotes the desorption of CH4. The mechanism of N2 injection and CH4 desorption in coal seams mainly includes gas displacement and gas dilution and diffusion. This study provides theoretical support for methane extraction technology in goaf.
A gut-derived hormone suppresses sugar appetite and regulates food choice in Drosophila
Authors: Malita Alina , Kubrak Olga , Koyama Takashi , Ahrentløv Nadja , Texada Michael J. , Nagy Stanislav , Halberg Kenneth V. , Rewitz Kim 
Published: November 2022

AbstractAnimals must adapt their dietary choices to meet their nutritional needs. How these needs are detected and translated into nutrient-specific appetites that drive food-choice behaviours is poorly understood. Here we show that enteroendocrine cells of the adult female Drosophila midgut sense nutrients and in response release neuropeptide F (NPF), which is an ortholog of mammalian neuropeptide Y-family gut-brain hormones. Gut-derived NPF acts on glucagon-like adipokinetic hormone (AKH) signalling to induce sugar satiety and increase consumption of protein-rich food, and on adipose tissue to promote storage of ingested nutrients. Suppression of NPF-mediated gut signalling leads to overconsumption of dietary sugar while simultaneously decreasing intake of protein-rich yeast. Furthermore, gut-derived NPF has a female-specific function in promoting consumption of protein-containing food in mated females. Together, our findings suggest that gut NPF-to-AKH signalling modulates specific appetites and regulates food choice to ensure homeostatic consumption of nutrients, providing insight into the hormonal mechanisms that underlie nutrient-specific hungers.