bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oviduct Epithelial Cells Constitute Distinct Lineages from the Ampulla to the Isthmus Related to High-Altitude Adaptation in Yak (Bos grunniens)
Authors: zhang jian , Cui Yan , pan yangyang , wang jinglei , wang meng , Yu Sijiu 
Published: November 2022

Abstract
High-altitude adaptation is viewed as a biologically based process during which yaks respond to the harsh condition of coldness and low oxygen. Although high-altitude adaptation has been studied intensively, there is a lack of multi-omics analysis on its physiological mechanism. Therefore, in this study, 10X Genomics single-cell technology were adopted to identify cellular heterogeneity and trajectory differentiation in different parts of the yak oviduct, thus further exploring new cell markers and ligand-receptors. Besides, based on the comparison of oviduct epithelial cells from the ampulla and isthmus between the yak and bovine, genes and pathways related to altitude adaptation were discussed by bulk-seq. Single cell RNA sequencing (scRNA-seq) revealed 14 cell types defined by multiple specific molecular markers, the main cell types are epithelial cells, ciliated cells, secretory cells, basal cells, mesenchymal stem cells, T cells, B cells, monocytes, NK cells, macrophages, and fibroblasts, among which basal cells could be differentiated into secretory cells and ciliated cells. Apart from that, immune cells were the apex products of cell development, while ciliated cells had the characteristics of de-differentiation, and the new ligand-receptors CD74-COPa and CD74-APP were found. In addition, by bulk-seq, it was found that in the ampulla, there were 5149 differential genes (1875 up-regulated genes and 3274 down-regulated genes). YME1L1 was down-regulated significantly, whereas PPIB, PCBD, CYTB, RPL24, RPS5, and UBL5 were up-regulated to a great extent. In the isthmus, there were 6313 differential genes (2146 up-regulated genes and 4167 down-regulated genes). Among them, YME1L1, GSTA4, DNTTIP2, TMCO6, LRBA, and MYH14 were down-regulated obviously, while SNRPD3, CYTB, ADIPOQ, RNH1, PRDX5, DPF1, CRYAB, and PRXL2B were up-regulated dramatically. Through the comparison, it was observed that GSTA4 and YME1L1 were down-regulated, while CYTB was up-regulated in the ampulla and the isthmus. Beyond that, the oxidative phosphorylation pathway was explored. In conclusion, it was demonstrated that ciliated cells were featured with dedifferentiation, and new ligand receptors CD74-COPa and CD74-APP were discovered. Furthermore, GSTA4, YME1L1 and CYTB were main molecules of altitude adaptation, and the oxidative phosphorylation pathway was the key pathway of high-altitude adaptation. This study provides basic data on the heterogeneity and cell trajectory of yak oviduct from the single-cell sequence, and provides the key genes and pathways of yak high-altitude adaptation, as well as the theoretical basis and the data for revealing the low pregnancy rate and genetic breeding of yaks.
The evolution of Earth’s surficial Mg cycle over the past 2 billion years
Authors: Xia Zhiguang , Li Shilei , Hu Zhongya , Bialik Or , Chen Tianyu , Weldeghebriel Mebrahtu , Fan Qishun , Fan Junxuan , Wang Xiang-dong , An Shichao , Zhang Feifei , Xu Haoran , Chen Jiayang , Ji Zhihan , Shen Shu-zhong , Lowenstein Tim , Li Weiqiang 
Published: November 2022

Abstract
The surficial cycling of Mg is directly coupled with the global carbon cycle, a predominant control of Earth’s climate. However, how Earth’s surficial Mg cycle evolved with time had been elusive. Magnesium isotope signatures of seawater (δ26Mgsw) track the surficial Mg cycle, which could provide crucial information on the carbon cycle in Earth’s history. Here, we present a reconstruction of δ26Mgsw evolution over the last 2 billion years using marine halite fluid inclusions and sedimentary dolostones. The two independent archives yield consistent evolutionary trends of δ26Mgsw for the past 430 million years, and the dolostone records extend the δ26Mgsw curve to 2 billion years ago. Modeling results of the net CO2 sequestration efficiency (EMg−CO2) by the surficial Mg cycle based on the δ26Mgsw record reveal a secular decline EMg−CO2 during the past 2 billion years, with the periods of low EMg−CO2 coinciding with ice ages in the Phanerozoic. Our work underlines a previously under-appreciated, but indispensable role of dolostones in regulating Earth’s climate on geologic time scales.
Effects of Salmonella enteritidis Infection on TLR Genes Expression and Microbial Diversity in Laying Hens
Authors: Jiang Xintong , Zhang Xiao , Sun Yuqing , Sun Zhongtao , Liu Liying , Li Xianyao 
Published: November 2022

Abstract
Background: Salmonella enteritidis (SE) is one of the critical foodborne pathogens, and mainly causes human diseases through contaminated water and food. In the current study, serological tests, histopathological observation and quantitative real-time PCR were used to assess the effect of Salmonella enteritidis infection on the immune system in chickens. To understand the influence of SE infection on the microflora profile of the cecum and oviduct, microbial community composition of the cecal contents and oviducal contents wereanalyzed through 16S rRNA sequencing.
Results: Results showed that SEinfection caused damage to the digestive organs, reproductive organs, and immune organs in laying hens. The expression of TLR1a, TLR1b, TLR2, TLR4, TLR5, TLR7 and TLR15 in the cecum were induced, and the content of IFN-γ, TNF-α, IL-2 and IL-18 in serum were increased after SE infection. The composition of the microbial community significantly changed in cecal content, the dominant bacteria of Firmicutes increased, and Bacteroidetes decreased significantly. In the oviduct, the microbial diversity became complicated, the dominant bacteria Faecalibacterium was significantly increased, and Bacteroides was significantly decreased.
Conclusions: Salmonella enteritidis infection triggered the host’s innate and adaptive response, increased the expression of TLRs and contents of cytokines and changed the microbial diversity in the cecum and oviduct. These findings willlay the foundation for further study on reducing the vertical transmission of Salmonella enteritidis, ensuring the production of clean eggs.
A rise in social media use in adolescents during the COVID-19 pandemic: the French validation of the Bergen Social Media Addiction Scale in a Canadian cohort
Authors: Dufort-Rouleau Raphaël , Beauregard Carmen , Beaudry Vincent 
Published: November 2022

Abstract

Introduction:
Social media use has grown dramatically since its inception in the early 2000s and has further increased during the COVID-19 pandemic. Problematic use of social media (PUSM) is a type of behavioural addiction which has generated increasing interest among mental health clinicians and scholars in the last decade. PUSM is associated with multiple psychiatric conditions and is known to interfere with patients’ daily functioning. There is no single accepted definition of PUSM, nor means of measuring it, in the literature. The Bergen Social Media Addiction Scale (BSMAS) is a helpful tool for identifying PUSM. This paper aims to validate BSMAS and to translate it from English into French, with the goal of making this clinical screening tool for PUSM available in French-language contexts.
Method
This study explored the psychometric validity of the French version of the BSMAS in a sample of 247 adolescents, who were either psychiatric inpatients (the hospitalized group) or recruited in local high schools (the community group).
Results
The adolescents in the sample reported an increase in their social media use during the COVID-19 pandemic. This increase was more pronounced in the hospitalized group. Confirmatory factorial analysis showed an excellent fit, very good internal consistency and established convergent validity for the French version of the BSMAS. A total of 15,4% of the hospitalization group and 6,5% of the community group met the threshold of 24 on the BSMAS, suggesting problematic use of social media.
Conclusions
the French version of BSMAS is a psychometrically validated and clinically useful tool to screen for PUSM in adolescents.
Numerical and experimental study to predict the forming process conditions of non-axisymmetric glass perfume bottles
Authors: Biosca Adrià , Borros Salvador , Clemente Vicenç Pedret , Hyre Matthew R. , Granada Andres Amador Garcia 
Published: November 2022

Abstract
After the successful validation of the axisymmetric and three-dimensional models to simulate the blow and blow forming process using two axisymmetrical bottles, the capabilities of the 3D model have been evaluated by performing simulations with more complex geometries. This article presents the results of a numerical and experimental study of a non-axisymmetrical glass bottle. In this study, a perfume bottle with a square-based prism geometry and a new set of industrial process conditions have been evaluated. To validate the numerical results, the predicted temperatures in the glass domain have been correlated with experimental infrared measurements of the glass at various stages of the forming process. A comparison of the predicted glass thickness distributions with the section profiles obtained from the manufactured glass bottles is also provided. Finally, transition from the 2Da to 3D model resulted in new difficulties and capabilities that are also discussed.
Identification of lung adenocarcinoma subtypes and a prognostic signature based on activity changes of the hallmark and immunologic gene sets
Authors: Zhou Shunkai , Zeng Dehua , Zhang Meiqing , Chen Mengmeng , Liu Yaming , Chen Qiqiang , Lin Zhenya , Yang Shengsheng , Fu Zhichao , Ying Wenmin , Lian Duohuang 
Published: November 2022

Abstract

Background: Lung adenocarcinoma (LUAD) has a complex tumor heterogeneity. This study aimed to identify LUAD subtypes and build a reliable prognostic signature based on the activity changes of the hallmark and immunologic gene sets.
Methods: Changes in the activities of the hallmark and immunologic gene sets were analyzed based on The Cancer Genome Atlas (TCGA)-LUAD dataset, followed by identification of prognosis-related differential gene sets (DGSs) and their related LUAD subtypes. Survival analysis, correlation with clinical characteristics, and immune microenvironment assessment for subtypes were performed. Moreover, the DGSs among different subtypes were identified, followed by the construction and evaluation of a prognostic risk score model and nomogram. The tumor mutation burden (TMB) of different risk groups wascompared.
Results: Two LUAD subtypes were identified based on the activity changes of the hallmark and immunologic gene sets. Cluster 2 had worse prognosis, more advanced tumorand clinical stages, and higher immune infiltration than cluster 1. Moreover, a prognostic risk score signaturewas established using two LUAD subtype-related DGSs, which could stratify patients at different risk levels. A shorter survival time and higher TMB levels were observed in the high-risk patients. The established nomogram accurately predicted the survival outcomes.
Conclusions: Our findings revealed that our constructed prognostic signature could accurately predict the survival outcomes and immune microenvironment of patients with LUAD, which was helpful in predicting the prognosis and guiding personalized therapeutic strategies for LUAD.
Psoralidin inhibits osteosarcoma function by down-regulating ITGB1 expression through FAK and PI3K/Akt signaling pathways
Authors: Cheng Shengwen , Liu Senrui , Chen Bowen , Du Chengcheng , Xiao Pengcheng , Luo Xuefeng , Wei Li , Lei Yiting , Zhao Chen , Huang Wei 
Published: November 2022

Abstract
Background
Psoralea is a medicinal plant of legume, which has been used in many diseases for a long time. Psoralidin (PSO) is the main extract of psoralea, which has antibacterial, anti-tumor, anti-inflammatory, antioxidant and other pharmacological activities. The inhibitory effect of PSO on tumor has been found, but its inhibitory effect on osteosarcoma has not been reported. Therefore, this study aimed to evaluate the inhibitory effect of PSO on osteosarcoma and its underlying molecular mechanism.
Materials and Methods
Crystal violet assay, CCK8 assay, and EdU stain assay were used to confirm the inhibitory effect of PSO on the proliferation of 143B and MG63 osteosarcoma cells. Wound healing and Transwell assays were conducted to evaluate the effects of PSO on osteosarcoma cell migration and invasion. The cell cycle and apoptosis were observed by flow cytometry. RNA sequencing was performed to determine the possible relevant molecular mechanisms, and protein expression levels were analyzed using Western blot. The inhibitory effect of PSO on osteosarcoma in vivo was analyzed by an orthotopic OS tumor animal model and immunohistochemistry.
Results
According to crystal violet assay, cck8 assay, and EdU stain assay, PSO inhibited cell proliferation in a concentration-dependent manner. Moreover, PSO inhibited the migration and invasion of the osteosarcoma cells. Flow cytometry analysis showed that PSO induces cell cycle arrest and apoptosis in OS cells. To elucidate the molecular mechanism of PSO, we performed RNA-seq analysis and found that PSO treatment significantly inhibited FAK and PI3K/Akt signaling pathways by down-regulating the expression of ITGB1 in MG63 and 143B cells. Furthermore, we confirmed that PSO restrained osteosarcoma growth In vivo mouse models.
Conclusion
PSO may play an anti-osteosarcoma role via FAK and PI3K/Akt signaling pathways by down-regulating ITGB1 expression
A Real-time Calibration Method for the Numerical Pollen Forecast Model COSMO-ART
Authors: Adamov Simon , Pauling Andreas 
Published: November 2022

Abstract
Technologies for monitoring pollen concentrations in real time made substantial advances in the past years and become increasingly available. This opens the possibility to calibrate numerical pollen forecast models in real time and make a significant step forward regarding the quality of pollen forecasts. We present a method to use real-time pollen measurements in numerical pollen forecast models. The main idea is to calibrate model parameterizations and not to assimilate measurements in a nudging sense. This ensures that the positive effect persists throughout the forecast period and does not vanish after a few forecast hours. We propose to adapt in real time both the model phenology scheme and the overall tuning factor that is present in any numerical pollen forecast model. To test this approach we used the numerical pollen forecast model COSMO-ART (COnsortium for Small-scale MOdelling - Aerosols and Reactive Trace gases) on a mesh size of 1.1 km covering the greater Alpine domain. Test runs covered two pollen seasons and included Corylus, Alnus, Betula and Poaceae pollen. Comparison with daily measurements from 13 Swiss pollen stations revealed that the model improvements are large, but fine tuning of the method remains a challenge. We conclude that the presented approach is a first valuable step towards comprehensive real-time calibration of numerical pollen forecast models.
High Resolution Melting (HRM) approach-based strategy for simultaneous differentiation of five Babesia species response for human babesiosis
Authors: Wang YanBo , Zhang Shangdi , Li Xiaoyun , Nian Yueli , Yin Hong , Liu Xinyue , Liu Junlong , Wang Jinming , Guan Guiquan 
Published: November 2022

Abstract
Background
Five Babesia species, including Babesia microti, Babesia divergens, Babesia duncani, Babesia motasi, and Babesia crassa-like, are considered to be the major pathogens responsible for human babesiosis in endemic regions. Each of these species possesses a variable degree of virulence towards its hosts. Therefore, the accurate identification of these species is critical for the adoption of appropriate therapies.
Methods
In this study, we developed a real-time PCR high-resolution melting analysis (qPCR- HRM) approach targeting 18S rRNA for distinguishing the five Babesia spp. based on melting temperature (Tm) and genotype confidence percent (GCP) values. This approach was evaluated using 429 blood samples from patients with a history of tick bites and 200 positive specimens from laboratory infections.
Results
The results showed that the sensitivity and specificity of the proposed qPCR-HRM based method were 95% and 100%, respectively, and the detection limit weas 1-100 copies of plasmid. The detection performance of the assay was assessed using clinical and laboratory infected specimens.
Conclusions
The developed detection method provides a useful tool for the epidemiological investigation of human babesiosis and pre-transfusion screening.
Sonoprinting Nanoparticles on Cellular Spheroids via Surface Acoustic Waves for Enhanced Nanotherapeutics Delivery
Authors: Tabrizian Maryam , Rasouli Reza , Paun Radu 
Published: November 2022

Abstract
Nanotherapeutics, on their path to the target tissues, face numerous physicochemical hindrances that affect their therapeutic efficacy. These barriers can limit the penetration of nanocarriers into deeper regions of densely packed tissues such as tumors, thereby preventing the efficient delivery of drug cargo. To address this challenge, we introduce a novel approach that employs surface acoustic wave (SAW) technology to sonoprint and enhance the delivery of nanoparticles to spheroids. Our SAW platform is designed to generate focused and unidirectional acoustic waves for creating vigorous acoustic streaming and Bjerknes forces. The effect of SAW excitation on cell viability as well as the accumulation and penetration of nanoparticles on human breast cancer (MCF 7) and mouse melanoma (YUMM 1.7) spheroids were investigated. The high frequency, low input voltage, and contact-free nature of the proposed SAW system ensured over 92% cells’ viability. The SAW sonoprinting enhanced the accumulation of 100 nm polystyrene particles on the spheroids to near four-fold, while improving the penetration of nanoparticles into the spheroids by up to three times. To demonstrate the effectiveness of our SAW platform for nanotherapeutics delivery, the platform was used to deliver nanoliposomes encapsulated with the anti-cancer copper diethyldithiocarbamate (CuET) to MCF 7 and YUMM 1.7 spheroids. A three-fold increase in the cytotoxic activity of the drug was observed in spheroids under SAW, compared to controls. The capacity of SAW-based devices to be manufactured as minuscule wearable patches can offer highly controllable, localized, and continuous acoustic waves to enhance drug delivery efficiency to target tissues.
Analytical Approaches to Characterize the Source of Meiyuanshi-stone Using Petrographic and Geochemical Methods
Authors: Guo Xiuwei , Zhang Yawen , Xu Xuemin , Dai Shibing , Huang Jizhong 
Published: November 2022

Abstract
The "Meiyuanshi-stone", who has witnessed the prosperous of the Maritime Silk Road, has attracted the attention of scholars in China, Japan, and South Korea in recent years. Meiyuanshi-stone was prestige material for carving, figures or building elements of temples. Not only for authentical conservation of such stone monuments, the features of Meiyuanshi-stone provide the most direct physical information for confirming similar stone materials in China, Japan, and countries around the Maritime Silk Road. Aiming to define Mieyuanshi-stone, petrographic and geochemical investigations of rocks were carried out on samples collected from quarries and the Sumeru seat in the main hall of Baoguosi (Ningbo, Zhejiang, China). The integrated chemical data acquired by inductively coupled plasma-mass spectrometry (ICP-MS) and X-Ray Diffraction (XRD) analysis, along with petrographic data, allowed us to ascertain the compositions of raw materials. Through comparison of petrographic features, major and trace elements contents of Meiyuanshi-, Xiaoxishi-, and Dayinshi-stone, it has been concluded that Meiyuanshi-stone is massive (no internal stratification), thick structureless, well sorted, and fine-grained trachytic tuff. By comparing petrographic and chemical features, we were able to confirm that the Sumeru seat in Baoguosi is made of Meiyuanshi-stone.
Sampling Point Planning for Complex Surface Inspection based on Feature Points under Area Division
Authors: Sun Jianghao , Xiang Sitong , Zhou Tao , Cheng Tao 
Published: November 2022

Abstract
Traditional sampling point planning methods usually apply the same sampling strategy to all areas of the complex surface. It is very likely to miss the extreme points in areas with large curvature changes, which leads to low fitting accuracy. This paper proposes a new sampling method for complex surfaces based on feature points under area division. First, the curvature characteristics of the complex surface is analyzed, and the complex surface is divided into flat and sharply-edged areas. Then, for the flat areas the uniform distribution method is used, and for the sharply-edged areas, the feature points are defined and searched, and the curvature adaptive planning method based on the feature points is adopted. Finally, the repeated and redundant points are optimized and adjusted. The experimental verification results show that compared with four traditional sampling methods, the maximum and mean fitting errors of the proposed method are significantly reduced and the measuring accuracy is efficiently improved.
Performance Evaluation and Analysis of Massive MIMO channels for 5G Mobile Networks
Authors: KABAOU Mohamed Ouwais , Zoghlami Nesrine , Hafidhi Intidhar , Khan Ilyas 
Published: November 2022

Abstract
The main objective of this study was to study, analyze and address the channel behavior in a mobile radio transmission chain. One of the major challenges in telecommunications networks (5G) is to minimize the propagation attenuation and consequently guarantee good Quality of Services. With the increased demand in terms of throughput and etwork coverage, a rigorous characterization of channels is demanded. In this article, a new characterization method that uses an analytical modeling approach based on Multiple Input Multiple Output (MIMO) channels is proposed. Definitely, the channel characterization and modeling for a MIMO system must take into account at the same level the spatial and temporal representations. In the spatial domain, the transmission channel characterization allows realistic and accurate modeling. In a MIMO context, this modeling is important since it gives a gain in throughput and, consequently, a good performance relatively to conventional and classical systems. Here, it is studied the behavior of these information carriers deploying the context of propagation environment as well as the effect of fading, diversity, multiplexing and multipath phenomenon. Hence, a specific study based on Rayleigh and Additive White Gaussian Noise channels is presented.
Tensor splitting preconditioners for multilinear system
Authors: Karimi Saeed , Dehdezi Eisa Khosravi 
Published: November 2022

Abstract
In this paper, we propose some new preconditioners for solving multilinear system Axm-1 = b. These preconditioners are based on tensor splitting. We also present some theorems for analyzing and converging the preconditioned Jacobi, Gauss-Seidel, and SOR-type iterative methods. Numerical examples are presented to verify the efficiency of the proposed preconditioned methods.
Mathematics Subject Classification (2010). 15A10, 15A69, 15A72, 15A99, 65F10.