bluuebunny/crossref_metadata_2025_split huggingface dataset:
GENÉTICA MOLECULAR DOS GLIOMAS CEREBRAIS: IMPORTÂNCIA NA CLASSIFICAÇÃO, SOBREVIDA E TRATAMENTO
Authors: FELIN CAROLLINA DANEZI , FELIN GIULLIANO DANEZI , FELIN GIANCARLLO DANEZI , FELIN FELLIPE DANEZI , FELIN IZABELLA PAZ DANEZI 
Published: August 2022

Introdução: No Brasil os gliomas representam 42% dos tumores cerebrais. Originam-se do parênquima cerebral e tem comportamento variado. Os de alto grau são agressivos e tem sobrevida média global de 15 meses. Os de baixo grau tem sobrevida de 2-5 anos, podem recidivar ou progredir para piores graus. Objetivos: Identificar a genética molecular dos gliomas e sua importância na classificação, sobrevida e tratamento desses tumores. Metodologia: Revisão de literatura através de pesquisa na base de dados MEDLINE, via PubMed, utilizando os termos: “glioma” [and] “molecular genetics” [and] “therapy”. Aplicados os filtros de busca: “textos completos gratuitos”, “dados associados” e “últimos 5 anos”. Foram encontrados 22 resultados e incluídos 6 para esse estudo. Os critérios de elegibilidade foram todos os artigos coincidentes com tema proposto conforme os filtros e termos de busca. Excluiu-se 16 artigos por não contemplarem os critérios elegíveis. Realizada extração de dados, análise dos resultados e redação dessa revisão. Resultados: No contexto do glioma estão os seguintes tumores: astrocitoma, oligodendroglioma, glioblastoma multiforme e ependimoma. Há marcadores genéticos específicos que caracterizam os astrocitomas de acordo com os graus de I até IV. Os glioblastomas secundários apresentam mutações nos genes IDH1 ou IDH2 diferentemente da apresentação primária. Os astrocitomas e os oligodendrogliomas podem expressar mutações dos genes IDH1 ou IDH2. Os oligodendrogliomas frequentemente tem mutação IDH1/2 e codeleção 1p/19q, enquanto que os astrocitomas tem mutação dos genes ATRX, pTP53 e IDH1/2, sem a codeleção 1p/19q. A codeleção 1p/19q é característica dos tumores oligodendrogliais, útil no diagnóstico e também como preditor de melhor sobrevida e melhor resposta à radioterapia e à quimioterapia. A presença da mutação IDH1/2 relaciona o tumor a um melhor prognóstico e melhor resposta à quimioterapia. Existem quatro graus do ependimoma e nove subtipos moleculares distintos, determinado variabilidade genotípica e fenotípica, determinando tratamento e prognóstico diversos. Conclusão: Foi possível identificar que o perfil genético molecular dos gliomas é determinante tanto para subclassificar quanto para predizer sobrevida e tratamento, pois a genética molecular heterogênea dos gliomas mostrou caracterizar doenças com fenótipos distintos. O conhecimento da genética molecular pode abrir novas oportunidades de tratamento indicando estratégias mais específica de tratamento.
ANÁLISE DE PRESENÇA E ATIVIDADE DO GENE TERT DA TELOMERASE EM CROMOSSOMOS B DE PSALIDODON PARANAE (TELEOSTEI, CHARACIDAE)
Authors: Nadai Pamela Cristina Ferreira de , VIDAL MATEUS ROSSETTO , LASMAR LUCAS FORTINO , OLIVEIRA CLAUDIO , FORESTI FAUSTO 
Published: August 2022

Introdução: Diversos organismos eucariotos possuem elementos genômicos extras, conhecidos como cromossomo B. Esses elementos são em geral heterocromáticos e compostos principalmente por sequências repetitivas de DNA, além de também possuírem genes ativamente transcritos. Os telômeros são estruturas cuja a função é manter a integridade estrutural dos cromossomos, e são constituídos por sequências não codificantes e repetitivas de DNA (TTAGGG). Neste sentido, a enzima telomerase, codificada pelo gene tert (telomerase RNA reverse transcriptase), desempenha um papel importante, atuando diretamente no elongamento dos telômeros. Considerando que os cromossomos B são elementos extras no genoma, pode-se supor que, por um mecanismo de compensação, uma maior atividade do gene tert seja induzida pela presença destes cromossomos. Objetivo: Deste modo, nosso objetivo foi analisar a presença do gene tert em cromossomo B de indivíduos Psalidodon paranae, tal como analisar se há expressão diferencial desse gene associado a presença do cromossomo B entre indivíduos 0B e B+. Metodologia: Para isso, foram coletados espécimes de P. paranae do ribeirão Cascatinha em Botucatu-SP (22°53’30”S 48°28’36”W), sendo 3 indivíduos B+ e 3 indivíduos 0B. Para análise da presença e da expressão do gene tert nesses indivíduos, foram utilizadas leituras curtas de alto rendimento de bibliotecas de DNA e RNA, obtidas através do sequenciamento Illumina. Em seguida, foi realizado o mapeamento das leituras usando o software SSAHA2 tendo a sequência codificadora do gene tert como referência. Foram usados scripts customizados para contabilizar o número de leituras mapeados e gerar os gráficos de cobertura. As análises bioinformáticas em bibliotecas de DNA e RNA não apresentaram diferença de cobertura entre as bibliotecas B+ e 0B, indicando ausência do gene tert no cromossomo B e ausência de expressão diferencial. Resultados: Nossos resultados indicam que os cromossomos B não exercem nenhuma influência na maquinaria de regulação telomérica. Pode-se especular, portanto, que estes elementos poderiam levar, em longo prazo, a efeitos deletérios. No entanto, para melhor elucidação desta hipótese estudos futuros comparando expressão gênica entre adultos e juvenis são necessários. Conclusão: Ainda, considerando que P. paranae possui no máximo dois cromossomos B, em um cenário similar estes efeitos poderiam ser intensificados em organismos com maiores números de cromossomos B.
EXPOSIÇÃO AO GLIFOSATO E OCORRÊNCIA DE ANOMALIAS CONGÊNITAS: UMA DÉCADA DE ESTUDOS EM ANIMAIS
Authors: Silva Iris Moreira da , ERRERA FLAVIA IMBROISI VALLE 
Published: August 2022

Introdução: As anomalias congênitas (AC) são uma das principais causas de mortalidade infantil e contribuem para problemas de saúde ao longo da vida. Fatores genéticos e ambientais contribuem para o desenvolvimento de AC. Dentre os fatores ambientais, a exposição a agrotóxicos pode causar inúmeros efeitos adversos para o meio ambiente, seres vivos e especialmente para a saúde dos seres humanos. Tanto antes quanto durante a gravidez, a exposição a agrotóxicos está associada à AC. Um dos agrotóxicos mais utilizados é o Glifosato, um herbicida cujas vendas e o uso aumentaram desde a introdução de novas variedades de culturas geneticamente modificadas. A associação entre a exposição ao Glifosato e a ocorrência de AC em humanos é pouco conhecida, mas vem sendo investigada, principalmente em animais e revelando não só desfechos metabólicos e reprodutivos negativos para adultos quanto também AC. Uma revisão desses achados em animais pode auxiliar nas pesquisas com seres humanos. Objetivo: realizar uma revisão sobre a associação entre a exposição ao Glifosato/Roundup e a ocorrência de anomalias congênitas. Metodologia: Foi realizada uma revisão integrativa da literatura. Foram selecionados artigos realizados em animais e escritos em Inglês, que foram publicados nos últimos 10 anos, como textos completos e livres. Os termos de busca utilizados foram: “congenital anomalies” OR “birth defects” OR “malformations” AND “pesticides” and Glyphosate. Resultados: Foram identificados 18 artigos, dos quais dois foram excluídos pela leitura do título e dois após a leitura do resumo. Ao final, 14 foram selecionados para análise nesta revisão. A maioria (13) dos artigos eram relacionados a animais, sendo apenas quatro com abordagem em humanos. Foram observados: um artigo sobre Truta, seis artigos em anuros, dois artigos com carpas, três com Danio rerio (Zebrafish), dois com ratos e apenas em coelhos. Um artigo abordou mais de uma espécie citada acima. Dentre as AC encontradas, as principais foram anormalidades cardíacas (4), craniofaciais (3), bucais (2) e associados à coluna vertebral/notocorda (2), bem como intestinais. Conclusão: Estudos em animais, embora menos escassos que em humanos mostram que a o Glifosato é associado à ocorrência de AC, indicando a importância de conhecer essa associação em humanos.
PM10 variation, composition, and source analysis in Tuscany (Italy) following the COVID-19 lockdown restrictions
Authors: Giardi Fabio , Nava Silvia , Calzolai Giulia , Pazzi Giulia , Chiari Massimo , Faggi Andrea , Andreini Bianca Patrizia , Collaveri Chiara , Franchi Elena , Nincheri Guido , Amore Alessandra , Becagli Silvia , Severi Mirko , Traversi Rita , Lucarelli Franco 
Published: August 2022

Abstract. To control the spread of COVID-19, exceptional restrictive measures were taken in March 2020 that imposed a radical change on the lifestyle of millions of citizens around the world, albeit for a short period. The national lockdown, which lasted from 10 March to 18 May 2020 in Italy, was a unique opportunity to observe the variation in air quality in urban environments under conditions comprising almost total traffic restriction and a strong reduction in work activities. In this paper, the data from 17 urban monitoring sites in Tuscany are presented, and the PM and NO2 concentrations in the 2 months before the start of the lockdown and the 2 months after lockdown are compared with the corresponding months of the previous 3 years. The results show that the total loads of PM2.5 and PM10 (particulate matter with an aerodynamic diameter smaller than 2.5 and 10 µm, respectively) decreased, but they did not exhibit significant changes compared to previous years, whereas NO2 underwent a drastic reduction. For three of these sites, the chemical composition of the collected samples was measured using thermal–optical techniques, ion chromatography, and particle-induced X-ray emission analysis, and the application of multivariate positive matrix factorization analysis also allowed for PM10 source identification and apportionment. Using these analyses, it was possible to explain the low sensitivity of PM10 to the lockdown effects as being due to different, sometimes inverse, behaviors of the different sources that contribute to PM. The results clearly indicate a decline in pollution levels related to urban traffic and an increase in the concentration of sulfate for all sites during the lockdown period.
Late Cretaceous–early Palaeogene inversion-related tectonic structures at the northeastern margin of the Bohemian Massif (southwestern Poland and northern Czechia)
Authors: Głuszyński Andrzej , Aleksandrowski Paweł 
Published: August 2022

Abstract. A brief, regional-scale review of the Late Cretaceous–early Palaeogene inversion-related tectonic structures affecting the Sudety
Mountains and their foreland at the NE margin of the Bohemian Massif is presented
and complemented with results of new seismic studies. The Sudetes expose
Variscan-deformed basement, partly overlain by post-orogenic Permo-Mesozoic
cover, containing a wide spectrum of tectonic structures, both brittle and
ductile, in the past in this area referred to as young Saxonian or Laramide.
We have used newly reprocessed legacy seismics to study these structures in
the two main post-Variscan structural units of the area: the North Sudetic
and Intra-Sudetic synclinoria. The results were discussed together with
regionally distributed examples of tectonic structures from quarries and
underground mines as well as those known from the literature. The Late
Cretaceous–early Palaeogene tectonic structures in consecutively reviewed
Sudetic tectonic units, from the north to the south, typically include gentle to
moderately tight buckle folds, locally of detachment type or fault-related
and high-angle reverse and normal faults, as well as low-angle thrusts –
often rooted in the crystalline basement. The structures termed grabens in
the local literature are at the same time frequently interpreted as bounded
by reverse faults (hence we use here the term “reverse grabens”) and
typically reveal a strongly synclinal pattern of their sedimentary fill. The
top of the crystalline basement, as imaged by seismic data in the North Sudetic
Synclinorium below the faulted and folded cover, is synformally down-warped
with a wavelength of up to 30 km, whereas on the elevated areas, where the
basement top is exposed at the surface, it is tectonically up-warped (i.e.
antiformally buckled). The compressional structures typically show an
orientation that fits the regionally known Late Cretaceous–early
Palaeogene tectonic shortening direction of NE–SW to NNE–SSW. The same
applies to the regional jointing pattern, typically comprising an orthogonal
system of steep joints of ca. NW–SE and NE–SW strikes. All the reviewed
structures are regarded as being due to the Late Cretaceous–early Palaeogene
tectonic shortening episode, although some of the discussed faults with a
strike-slip component of motion may have been modified, or even produced, by
late Cenozoic tectonism.
A FORMULATION OF MANALAGI (Pyrus malus var. sylventris. Mill) APPLE EXTRACT TOOTHPASTE GEL PREPARATION WITH VARIATIONS OF CMC NA CONCENTRATION AS ANTIBACTERIAL AGAINST Streptococcus mutans ATCC 25175
Authors: - Anita Nilawati 
Published: August 2022

Background. Dental caries can be experienced at various ages which is marked by the appearance of white spots on the tooth surface by S. mutans bacteria. Manalagi apples contain compounds that are effective as antibacterial, namely polyphenols, saponins, alkaloids, tannins and flavonoids. Gel toothpaste formula with variations in CMC-Na of F1(1.5%), F2 (2%), and F3 (2.5%).
Purpose. The purpose of this study was to determine the effect of variations in CMC-Na concentration on the physical quality and antibacterial activity of S. mutans in the Manalagi apple extract gel toothpaste.
Methods. In addition, apple simplicia was extracted using 70% ethanol. Manalagi apple extract 15% was formulated into 3 formulas F1, F2, and F3 with CMC-Na concentrations of 1.5, 2, and 2.5%, respectively. Toothpaste gel preparations were tested for physical quality for 21 days, stability test for 3 cycles and antibacterial activity test against S. mutans by disc diffusion method. The data were processed with Shapiro-wilk statistics, ANOVA and Krustal Wallis, followed by the Paired t test and Wilcoxon
Results. The study showed that F1, F2, F3 affected the physical quality, among others, caused a decrease in the dispersion area but increased viscosity, pH and vice versa, and the Manalagi apple extract gel toothpaste with varying concentrations had antibacterial activity with inhibition zones 39 , respectively. 8; 39.6 and 37.3 mm, and the best formula was F1.
Conclusion. Manalagi apple extract has activity in inhibiting the growth of S. Mutans bacteria with a strong to very strong category, statistically there is a significant difference between variations in the concentration of CMC-Na.
GAMBARAN TINGKAT KECEMASAN MAHASISWA PRODI DIII KEPERAWATAN DALAM TUGAS AKHIR MASA PANDEMI COVID-19
Authors: Aseta Pandu , Siswanto  
Published: August 2022

Latar Belakang: Setiap akhir masa pendidikan, mahasiswa Prodi DIII Keperawatan dituntut untuk mengerjakan tuga akhir yang biasa disebut dengan KTI. Salah satu hal yang menjadikan kendala dalan penyusunan kasus di masa pandemi Covid-19 adalah terbatasnya akese beberpa tempat untuk melakukan study kasus untuk memnyusun KTI..Kecemasan juga akan dialami mahasiswa yang umumnya menginjak usia remaja yang masih labil dalam menghadapi situasi tak terduga.
Tujuan : Mengetahuai tingkat kecemasan mahasiswa menghadapi tugas akhir dimasa pandemic.
Metode: penelitian ini merupakan penelitian deskriptif dengan pendekatan cross-sectional. Populasi merupakan semua mahasiswa semester VI program studi D3 Keperawatan tahun 2022 yang berjumlah 84 responden dengan tehnik total sampling.
Hasil : Gambaran tingkat kecemasan mahasiswa program studi DIII Keperawatan semester IV yang mengalamai kecemasan sedang sebanyak 40 responden (80%), mahasiswa yang mengalami kecemasan baik ringan ringan 10 responden (20 %).
Kesimpulan: Tingkat kecemasan mahasiswa Prodi DIII Keperawatan semester IV yang menyusun tugas akhir paling banyak adalah sedang sebanyak 40 (75%) responden.
Saran : Diharapkan bagi mahasiswa Politeknik Insan Husada Surakarta untuk mampu melakukan manajemen stress dan waktu sehingga mampu menghindari atau mengurangi kecemasan dalam menyusun tugas akhir.
GAMBARAN TINGKAT PENGETAHUAN IBU DALAM PENCEGAHAN STUNTING PADA ANAK USIA TOODLER DI KEDUNGTUNGKUL MOJOSONGO SURAKARTA
Authors: Lestari Sri , Siti Nur Solikah  , POLITEKNIK INSAN HUSADA SURAKARTA  
Published: August 2022

Background. Stunting is a chronic malnutrition problem characterized by short stature. Stunting sufferers are generally susceptible to disease, have a below normal level of intelligence and low productivity. Mother's knowledge is one of the factors that have a significant influence on the incidence of stunting. Surakarta City will be the locus of stunting in 2021, Based on the results of stunting mapping and analysis by the Surakarta Health Office, Mojosongo ranks 8th with 5% cases.

Purpose. This study aims to describe the level of knowledge of mothers in preventing stunting in toddlers in Kedungtungkul, Mojosongo, Surakarta. Methods. This research is a quantitative research with descriptive method, the respondents in this study are mothers who have toddler age children in Kedungtungkul, Mojosongo, Surakarta. The sampling technique used was total sampling with 37 respondents. Result. (1) Characteristics of respondents based on age, most of them are 21 – 30 years old 41 %, Most education is SMA/SMK 54%, housewife work 65%. (2) The average level of knowledge of mothers in stunting prevention is good with the result that 65% of respondents' knowledge level is good.

Conclusion. Mother's level of knowledge in preventing stunting in Kedungtungkul Mojosongo Surakarta on average is good
Effectiveness of electromyography guided botulinum toxin injection and dextrose prolotherapy in recurrent TMJ dislocation (radiographic and clinical study)
Authors: Moussa Moataz Soliman , Elhasan Mohsen Fawzy Abo , Abdullah Abdel Aziz Baiomy 
Published: August 2022

Objectives: This study was conducted to assess effectiveness of dextrose prolotherapy, Electromyography (EMG) Guided Botulinum Toxin – A (BTX-A) Injection, and combination between them in treatment of recurrent TMJ Dislocation cases. Subjects and methods: This current study was carried out on 60 adult patients suffering from recurrent TMJ Dislocation. The patients divided equally into three groups, Group I: 20 patients were treated with EMG guided BTX-A injection. Group II: 20 patients were treated with dextrose prolotherapy. Group III: 20 patients were treated with combination of EMG guided BTX-A injection and dextrose prolotherapy. The clinical evaluation preoperatively and postoperatively (at 1week , 3 months , 6 months) included visual analog scale of TMJ pain, maximum mouth opening (MMO), and frequency of luxations . Radiographic evaluation using MRI was done preoperatively, and at 6 months. The collected data were then statistically analyzed. Results: best results were recorded in group III compared with other groups; at all intervals of study with significant reduction of pain score, and frequency of luxations beside to improvement of MMO measurements. Conclusion: the combination of EMG guided BTX-A injection and dextrose prolotherapy is a promising treatment in recurrent TMJ dislocation cases.
association between body mass index and severity of coronavirus disease 2019 (COVID-19)
Authors: Hamza Adnan , Shah Naveed Nazir , Farooq Syed Suraiya 
Published: August 2022

Background and purpose: The coronavirus disease 2019 (COVID-19) pandemic presents an unprecedented health crisis to the entireworld. As reported, the bodymass index (BMI)may play an important role in COVID-19; however, this still remains unclear. The aim of this study was to explore the association between BMI and COVID-19 severity and mortality. Methods: A retrospective, single-centred study including 88 participants with laboratory-detected SARS-CoV-2 infection admitted to designated COVID-19 centre in a tertiary care hospital from March to October, 2020 was done. Clinical records, laboratory data, and radiological findings were analysed. The impact OF BMI was evaluated to analyze their association with the severity of Covid-19 infection in these patients. Results: The severity of illness tended to increase in patients with higher BMI levels and lower BMI levels; the severity of illness in patients with BMI < 18.5 kg/m2 was highest (90%) followed by BMI > 25.0 kg/m2 was 79.2% when compared to patients with BMI 18.5–22.9 kg/m2 (77.8%).
DISSERTATION NURSING
Authors: Destrebecq Anne , Parozzi Mauro , Terzoni Stefano , Ferrara Paolo , Lusignani Maura 
Published: August 2022

In un momento storico in cui gli investimenti globali su ricerca e formazione sono estremamente ridotti, vogliamo cogliere un celebre invito:
“Osare. Il progresso si ottiene solo così”. (Victor Hugo)
Upaya Meningkatkan Keterampilan Menulis Bahasa Inggris melalui teknik EGRU pada siswa Kelas X SMA Negeri 2 Dompu
Authors: Chrisnawati Chrisnawati 
Published: August 2022

Penelitian ini bertujuan untuk meningkatkan keterampilan menulis siswa serta meningkatkan penguasaan pola-pola kalimat dan kosa kata dalam pembelajaran bahasa Inggris dan meningkatkan hasil belajar menulis siswa khususnya di kelas X-IPA SMA Negeri 2 Dompu semester genap tahun pelajaran 2019/2020, yang menjadi subyek penelitian ini adalah siswa kelas X-IPA SMA Negeri 2 Dompu semester genap tahun pelajaran 2019/2020, sebanyak 39 orang. Sedangkan obyek penelitian meliputi: EGRU, aktivitas siswa, hasil belajar siswa dan tanggapan siswa terhadap penerapan EGRU. Penelitian ini dilaksanakan dalam tiga siklus yaitu siklus I menampilkan teks pesan pendek dengan topik “Pengumuman”, sedangkan siklus II dan III menampilkan teks descriptive dengan topik “Description of person or things”. Pada kedua siklus menggunakan model pembelajaran Literacy yang berbasis EGRU, hasil analisis data didapat nilai rata-rata tes menulis pada siklus I adalah 65,38, nilai rata-rata tes menulis pada siklus II adalah 70,83 dan pada siklus III adalah 74,84. Pada siklus I terdapat 56,41% siswa tergolong aktif dan sangat aktif, pada siklus II terdapat 97,44% siswa tergolong aktif dan sangat aktif, sedangkan pada silkus III semua siswa nampak aktif. Berdasarkan angket tanggapan siswa, dari 39 orang, 36 orang menyatakan setuju digunakan EGRU dalam pembelajaran bahasa Inggris khususnya menulis, Berdasarkan penelitian tindakan kelas ini, EGRU sangat berpengaruh terhadap aktivitas, keterampilan menulis dan hasil belajar menulis siswa. Karena itu diharapkan guru-guru sejenis menggunakan EGRU dengan pendekatan Literacy pada pembelajaran bahasa Inggris khususnya menulis.
A critical analysis of the cultural differences of foreign employees working in China: a case study of the UK
Authors: Guo Jinchen 
Published: August 2022

This qualitative study proposes how to help cross-cultural communicators identify and address the causes and challenges of the cultural differences they encounter through the factors of cultural differences. The study was compared through a control group and data was collected and analysed by understanding interviewees' professional experiences to date as well as their experience of living in a foreign country. The participants were then asked specific questions using Hofstede's cultural dimensions theory and the concept of culture shock as a framework to inductively categorise the data while explaining through cultural dimensions theory and folk China theory.
This study provides new insights for cross-cultural communicators on the cultural differences between China and Britain and provides a new understanding of the cultural differences between China and Britain through the cultural dimension theory and folk China theory. This study integrates the existing theories, especially proves that folk China theory can be used as theoretical support for the cultural dimension of the Sino-British data, and summarizes the current influences and underlying reasons for the Sino-British cultural communicators. In addition, the study provides effective recommendations and suggestions for a new generation of Sino-British cross-cultural communicators, both individually and collectively.