bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell profiling identifies clinically relevant interactions between tumor associated macrophages and blood endothelial cells in diffuse large B cell lymphoma
Authors: Ferrant Juliette , Le Gallou Simon , Padonou Francine , Léonard Simon , Papa Ilénia , Pangault Céline , Barthel Anne , Launay Vincent , Manson Guillaume , Hoareau Bénédicte , Deleurme Laurent , Monvoisin Céline , Albaud Benoit , Van Acker Nathalie , Laurent Camille , Llamas-Gutierrez Francisco , Michaud Henri-Alexandre , Bonnefoy Nathalie , Pécot Thierry , Tarte Karin , Roussel Mikael 
Published: December 2022

SummaryDiffuse large B cell lymphoma (DLBCL) and follicular lymphoma (FL) are the two most common B-cell lymphomas and are characterized by a dynamic crosstalk between tumor B cells and a heterogeneous tumor-supportive microenvironment, including immune, endothelial, and stromal components. Although their impact on the pathogenesis and prognosis of B-cell lymphoma has been acknowledged for years, tumor-associated macrophages (TAM) have not been extensively explored in DLBCL and FL. Herein, we investigate mononuclear phagocytes (MNP) heterogeneity at the single cell level and their potential co-regulation with the stromal and endothelial compartments in B-cell lymphoma lymph nodes compared to reactive secondary lymphoid organs, using a combination of mass cytometry, single cell RNA sequencing, andin silicoapproaches. We reveal a co-regulation between TAM and blood endothelial cells (BEC) in lymphoma. Moreover, we identify a specific interaction between Annexin A1 (ANXA1)-expressing BEC and formyl-peptide receptors (FPR1/2)-expressing monocytes/macrophages in DLBCL, which we confirmin situby multiplex immunofluorescence and imaging mass cytometry. This crosstalk is associated to an immunosuppressive tumor microenvironment and an adverse prognosis in DLBCL.
Genomic data suggest parallel dental vestigialization within the xenarthran radiation
Authors: Emerling Christopher A. , Gibb Gillian C. , Tilak Marie-Ka , Hughes Jonathan J. , Kuch Melanie , Duggan Ana T. , Poinar Hendrik N. , Nachman Michael W. , Delsuc Frédéric 
Published: December 2022

AbstractThe recent influx of genomic data has provided greater insights into the molecular basis for regressive evolution, or vestigialization, through gene loss and pseudogenization. As such, the analysis of gene degradation patterns has the potential to provide insights into the evolutionary history of regressed anatomical traits. We specifically applied these principles to the xenarthran radiation (anteaters, sloths, armadillos), which is characterized by taxa with a gradation in regressed dental phenotypes. Whether the pattern among extant xenarthrans is due to an ancient and gradual decay of dental morphology or occurred repeatedly in parallel is unknown. We tested these competing hypotheses by examining 11 core dental genes in most living species of Xenarthra, characterizing shared inactivating mutations and patterns of relaxed selection during their radiation. Here we report evidence of independent and distinct events of dental gene loss in the major xenarthran subclades. First, we found strong evidence of complete enamel loss in the common ancestor of sloths and anteaters, suggested by the inactivation of five enamel-associated genes (AMELX, AMTN, MMP20, ENAM, ACP4). Next, whereas dental regression appears to have halted in sloths, presumably a critical event that ultimately permitted adaptation to an herbivorous lifestyle, anteaters continued losing genes on the path towards complete tooth loss. Echoes of this event are recorded in the genomes of all living anteaters, being marked by a 2-bp deletion in a gene critical for dentinogenesis (DSPP) and a putative shared 1-bp insertion in a gene linked to tooth retention (ODAPH). By contrast, in the two major armadillo clades, genes pertaining to the dento-gingival junction and amelogenesis appear to have been independently inactivated prior to losing all or some enamel. These genomic data provide evidence for multiple pathways and rates of anatomical regression, and underscore the utility of using pseudogenes to reconstruct evolutionary history when fossils are sparse.
A cosmopolitan inversion drives seasonal adaptation in overwinteringDrosophila
Authors: Nunez Joaquin C. B. , Lenhart Benedict A. , Bangerter Alyssa , Murray Connor S. , Yu Yang , Nystrom Taylor L. , Tern Courtney , Erickson Priscilla A. , Bergland Alan O. 
Published: December 2022

AbstractDrosophilamelanogasterliving in temperate regions evolve as they track seasonal fluctuations. Yet, we lack an understanding of the genetic architecture of seasonal adaptive tracking. By sequencing orchard populations collected across multiple years, we characterized the genomic signal of seasonal demography and identified that the cosmopolitan inversion In(2L)t drives seasonal adaptation. In(2L)t shows footprints of selection that are inconsistent with simple explanations of genetic drift, as well as signatures of partial selective sweeps. A meta-analysis of phenotypic studies shows that seasonal loci within In(2L)t are associated with behavior, life-history, physiology, and morphology traits. Our results identify candidate regions that underlie seasonal adaptive tracking and link them to phenotype. This work supports the general hypothesis that inversions are important drivers of rapid adaptation.One-Sentence SummaryA chromosomal inversion drives adaptive evolution between seasons in wild fruit flies.
Sex-specific deubiquitylation drives immune-related neurodegeneration inDrosophila
Authors: Xia Jingnu , Pinto-Fernández Adán , Damianou Andreas , Lee Jeffery Y , Kessler Benedikt M , Davis Ilan , Elliott Paul , Ligoxygakis Petros 
Published: December 2022

SummaryRisk of neurodegenerative disease such as late onset Alzheimer’s is linked to aberrant ubiquitinylation and accumulation of non-degraded proteins in brain cells. A glial network of innate immune genes modulates inflammatory responses to such protein deposition. However, vulnerability differs between the sexes. Here, we show that theDrosophilahomologue of the deubiquitylase Trabid can align the sex-specific aspects of neurodegenerative phenotypes with changes in ubiquitylation and inflammatory activity. An enzymatically null Trabid in flies, caused sex-specific changes in locomotion, sleep patterns, brain histology and ultimately, lifespan. These changes were underscored by altered ubiquitin and proteome enrichment profiles and the same enzymatic activity as its human counterpart. When the sex-determination genetransformerwas silenced in astrocytes or immunocompetent tissues, sex differences were significantly reduced. Our results indicate that Trabid underscores sex-specificity in disease neurology, by controlling the balance between ubiquitylation and inflammation.
A Very virulent infectious bursal disease virus closely related to New York strain isolated from vaccinated small-scale broiler poultry farm in Addis Ababa, Ethiopia
Authors: Almaw Gizat , Olani Abebe , Sombo Melaku , Yalew Bekele 
Published: December 2022

AbstractInfectious bursal disease virus (IBDV) was initially identified in the USA. The first IBD case in Ethiopia was reported in 2002 and since then vaccination was employed to control the disease. However, IBD outbreaks continued to occur despite the routine vaccination. The present study investigated IBD outbreak that had occurred in a vaccinated small-scale broiler poultry farm. Eight samples (four Bursa and four spleen) were collected and all were confirmed to be IBDV positive using reverse transcription-polymerase chain reaction (RT-PCR) targeting the hypervariable region of virus protein 2 (hVP2). All the IBDV isolates of this study were identified to be very virulent IBDV (vvIBDV) strains. For the four IBDV isolates the nucleotide and deduced amino acid sequence for hVP2 was determined. The nucleotide sequence identity of the VP2 gene of the present outbreak isolates (which showed 100% homology among themselves) with the previous 19 vvIBDV characterized isolates from Ethiopia ranged from 90.8% to 96.9% but greater than 96% identity was recorded with only six isolates. The deduced amino acid (aa) sequence of the present outbreak isolates contained aa residues commonly found in vvIBDV strains: A222, I242, I256, I294 and S299 suggesting the strains belong to genogroup three (G3). The phylogenetic analysis of the isolates showed that all isolates clustered separately from classical virulent, vaccine and variant strains and also distantly related to UK661 (UK) and DV86 (Netherlands) very virulent strains but unexpectedly closely related to the New York, USA strain. In conclusion, the present study reported vvIBDV strains (G3) isolated from a vaccinated broiler flock. Further research is needed to characterize the molecular epidemiology and pathogenicity of the vvIBDV strains and evaluate protective efficacy of the current IBD vaccine used for routine vaccination.
Inhibition of translation termination by Drosocin, an antimicrobial peptide from fruit flies
Authors: Mangano Kyle , Klepacki Dorota , Ohanmu Irueosa , Baliga Chetana , Huang Weiping , Brakel Alexandra , Krizsan Andor , Polikanov Yury S. , Hoffmann Ralf , Vázquez-Laslop Nora , Mankin Alexander S. 
Published: December 2022

AbstractA 19-amino acid longproline-richantimicrobialpeptide (PrAMP) Drosocin (Dro) is encoded in the fruit fly genome. Native Dro is glycosylated at a specific threonine residue, but the non-glycosylated peptide retains antibacterial activity. Dro shows sequence similarity to several other PrAMPs that bind in the ribosomal nascent peptide exit tunnel and inhibit protein synthesis by varying mechanisms. However, the target and mechanism of action of Dro remain unknown. Here we show that the primary mode of Dro action is inhibition of termination of protein synthesis. Our in vitro and in vivo experiments demonstrate that Dro stalls ribosomes at stop codons, likely sequestering class 1 release factors associated with the terminating ribosome. As the result, Dro strongly promotes readthrough of stop codons at subinhibitory concentrations. The elucidated mode of Dro action allows assigning it as the second member of the type II PrAMPs, of which only one representative, the antimicrobial peptide apidaecin (Api) produced by honeybees, was previously known. However, despite its functional similarity with Api, Dro interacts with the target in a markedly distinct way. The analysis of a comprehensive single-amino acid substitution library of endogenously expressed Dro variants shows that binding to the ribosome involves interactions of multiple amino acid residues distributed through the entire length of the PrAMP. Our data further show that the ribosome-targeting activity of non-glycosylated Dro can be significantly enhanced by single amino acid substitutions illuminating directions for improving its antibacterial properties.
Leadership and knowledge management practices for frugal innovation of firms in the emerging market: moderating role of collaborative culture
Authors: Le Phong Ba , Tran Dat Tho , Phung Thuy Minh Thu , Vu Khoa Dinh 
Published: December 2022

PurposeThe purpose of this study is to explore the influence of transformational leadership (TL) on firm's frugal innovation. It also deepens understanding of appropriate mechanisms and conditions to improve specific aspects of frugal innovation namely frugal functionality, frugal cost and frugal ecosystem by examining the mediating role of knowledge management capability (KMC) and moderating mechanism of collaborative culture.Design/methodology/approachThe paper utilized structural equation modeling and cross-sectional design to test hypotheses in the proposed research model using data collected from 351 participants in 112 Vietnamese firms.FindingsThe findings indicate that KMC significantly mediates TL's effects on aspects of firm's frugal innovation namely frugal functionality, frugal cost and frugal ecosystem. In addition, the influence of KMC on frugal functionality is different and depended on the extent of collaborative culture in an organization.Research limitations/implicationsThe paper has significantly contributed to increasing the understanding of the link between TL and specific aspects of frugal innovation by highlighting the important role of KMC and positive effects of collaborative climate in an organization.Originality/valueThe paper is unique in the attempts to provide the valuable initiatives and integration view of leadership practices for improving specific dimensions of frugal innovation of firms in developing and emerging market.
Matchmaking under uncertainty: how hiring criteria and requirements in professional work are co-created
Authors: Tholen Gerbrand 
Published: December 2022

PurposeThe aim of the study is to understand how the hiring process develops in cases where there are no explicit or formal requirements. How do implicit and informal criteria and requirements impact the process of selecting the right candidate?Design/methodology/approachA qualitative approach was employed through the use of semi-structured interviews with 47 external recruitment consultants in the south of England.FindingsIn contrast to what is assumed in mainstream Human Resource Management literature, employers do not rely on a comprehensive implicit understanding of what is needed in cases where there are no explicit criteria and requirements. Instead, high uncertainty makes the development of criteria and requirements incremental and negotiable but also problematic. The analysis shows that three mechanisms compensate for the lack of certainty in the hiring process. First, interviews with applicants shape how the hiring criteria develop. Second, market signals of what is available in the labour market help construct the criteria and requirements. Third, criteria and requirements are interpreted and negotiated during interactions with recruiters and others.Originality/valueHiring without explicit requirements and criteria is often understood as rather unproblematic and/or not fundamentally distinct from hiring with them. The study shows that in these cases the process becomes more unpredictable and more open to interpretation and negotiation.
Do commuters intend to avail electric street cars as public transport? Evidence from urban India
Authors: Dey Oindrila , Chakravarty Debalina 
Published: December 2022

PurposeElectric street car (ESC) is a globally popular clean and safe electric transport system for urban agglomeration. India envisions achieving “all-electric transport” by 2030, yet ESC as a modal transport alternative is not distinct in the policy discussion. The emerging market for electric transportation in urban spaces requires a detailed demand study at the service user level to remove behavioural barriers and design integrated energy planning in developing economies. This paper explores the probabilistic uptake intentions of the daily public transport commuters for ESCs over e-buses from the only Indian city with operational ESCs, Kolkata.Design/methodology/approachUsing a random utility model on primary survey data from daily commuters, the authors identify demographic, psychometric and socio-economic factors influencing probabilistic uptake of ESC over e-buses.FindingsIt estimates that 38% of the commuters demand ESC over e-buses, given the alternatives' comparative details. Factors like frequent availability and technological upgradation would increase the uptake of ESCs.Social implicationsThe study highlights that even though there are infrastructural challenges in the implementation of ESC, so does any other electric transport system; it is worth considering as a decarbonising transport alternative, given the high up-take intension of the users.Originality/valueThis is the first attempt to study the demand for ESC in developing economies, identifying the factors which may be considered in the sustainable urban transportation policy perspective.
The role of start-ups as knowledge brokers: a supply chain ecosystem perspective
Authors: Magliocca Pierpaolo , Herold David Martin M. , Canestrino Rossella , Temperini Valerio , Albino Vito 
Published: December 2022

Purpose
Existing literature is limited in its ability to consider start-ups as a knowledge broker to trigger innovation in a supply chain ecosystem (SCE). In a traditional SCE, start-ups are relatively isolated, leading to structural holes that limit knowledge sharing among members. This paper aims to overcome that limitation and to build frameworks that help to illustrate the interaction between knowledge management and sharing, start-up innovation and an ecosystem from a supply chain perspective.

Design/methodology/approach
Following a qualitative approach, this study theorizes about the role of start-ups as knowledge brokers and the implications of knowledge management and sharing with members in an SCE concerning innovation. Conceptual analysis is used for examination, and this study uses a set of qualitative tactics to interpret and generate meaning from the existing literature.

Findings
This study develops two frameworks to provide insight into how start-ups can trigger innovation as knowledge brokers in an SCE. The first framework shows how start-ups, and their knowledge base, influence supply chain members and the overall ecosystem, highlighting the isolated function of start-ups and the issue of structural holes in a traditional SCE. The authors propose a model that illustrates how structural holes can be bridged within an SCE, thereby demonstrating how start-ups redefine the ecosystem architecture according to their knowledge broker position in the SCE.

Originality/value
By expanding insight into the concepts of how start-ups can trigger innovation as knowledge brokers in an SCE, this paper extends the so-far neglected area of start-ups and knowledge brokers. This study clarifies the conceptual and theoretical components and processes in an SCE and links the different roles of start-ups as knowledge brokers to the respective supply chain members to better understand the implications on the entire SCE.
Robots in tourism and Sustainable Development Goals: Tourism Agenda 2030 perspective article
Authors: Ivanov Stanislav , Duglio Stefano , Beltramo Riccardo 
Published: December 2022

Purpose
The purpose of this perspective paper is to investigate the role of robots in tourism’s contribution to the achievement of the Sustainable Development Goals (SDGs).

Design/methodology/approach
With a deep analysis of the 17 SDGs and their 169 targets, the authors provide an interpretation on both the positive and negative impacts of robots in tourism towards the achievement of the SDG.

Findings
For several SDGs, the positive impact of robots in tourism is evident, whereas for others, it can be debated as the adoption of robots can lead to both positive and negative impacts, mostly dependent on how robots will be used. In particular, robots can provide a decent work environment for tourism and hospitality (TH) employees (SDG 8) who perform dangerous tasks while decreasing the consumption of resources (SDGs 2, 6, 7, 12, 13) due to their contribution to a company’s effectiveness and efficiency. In the meantime, robots have some impacts on the environment, both in terms of the use of resources (their manufacturing relies on rare Earth materials and robots are energy demanding) and output, especially for the management of toxic waste (e.g. batteries).

Research limitations/implications
The main limitation of this perspective paper is the lack of empirical measurement of the effects of robots in tourism on the achievement of the SDGs. However, it is a necessary exploration as a foundation for future research.

Practical implications
This perspective paper may be useful for robot manufacturers in identifying the positive and negative aspects of robots when designing sustainable robots (in terms of energy efficiency, management of the end-of-life of components, etc.) to conform to the expectations of the SDGs. It can be useful to TH companies when their managers evaluate whether to adopt robots in service operations or not.

Social implications
This contribution discusses the social implications in adopting robots in TH, specifically in terms of new opportunities for employment and in the substitution of humans in dirty, dull and dangerous tasks. It helps policymakers adopt the best policies for fostering the use of robots in TH with an eye to the environmental and social ramifications of robots.

Originality/value
To the best of the authors’ knowledge, this perspective paper is one of the first studies to investigate the role that robots can play in achieving the SDGs by TH companies.
Characteristics of pediatric continuous renal replacement therapies in hospitals with pediatric intensive care units in Japan
Authors: Haga Taiki , Ide Kentaro , Tani Masanori 
Published: December 2022

AbstractIntroductionInformation on the implementation of continuous renal replacement therapy (CRRT) in pediatric intensive care units (PICUs) is limited. We investigated the real‐world practice of this therapy in Japan.MethodsWe conducted a cross‐sectional survey of 26 hospitals with PICUs in Japan. One physician per hospital responded to the questionnaire.ResultsFewer than half of the hospitals followed the CRRT practice guidelines; treatment options were often selected at a physician's discretion. PICUs varied widely in continuous renal replacement settings, circuit priming methods, frequency of circuit exchange, and policies for using CRRT in sepsis. Only two anticoagulants were used: nafamostat mesylate and unfractionated heparin. Most units did not change the nutritional dosage according to CRRT use. Many facilities were proactive concerning rehabilitation.ConclusionNafamostat‐centered anticoagulation management was unique to Japan. Our results may help identify areas where additional clinical studies are needed to standardize CRRT practice.
Law and governance in the Anthropocene
Authors: Woolley Olivia , Harrington Cameron 
Published: December 2022

AbstractThis special issue on ‘Law and Governance in the Anthropocene’ brings together scholars from the disciplines of law and international relations to examine the ramifications of the Anthropocene for global governance and international law. The predominant focus of the literature to date has been understandably on defining the Anthropocene and on assessing what it means for the validity of longstanding viewpoints. However, more attention must be given to the specific changes needed in international relations and law in practice and as disciplines to adjust to the reality of human‐driven planetary change. Thus, it aims to build upon existing scholarship by developing specific governance responses to the challenges of the Anthropocene. This introductory article provides a brief outline of the larger workshop project which led to this special issue and offers a synopsis of the included articles. It concludes with some comments on the possible future directions for both scholarship and for legal and political practice.