bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of blastomere loss on pregnancy and neonatal outcomes of vitrified-warmed Day3 embryos in single embryo transfer cycles
Authors: Jiang Shutian , Jin Wei , Zhao Xinxi , Xi Qianwen , Chen Li , Gao Yining , Li Wenzhi , Kuang Yanping 
Published: May 2022

Abstract
Background
Blastomere loss is a common phenomenon that occurs following cryopreservation. To date, studies have drawn conflicting conclusions regarding the impact of blastomere loss on pregnancy outcomes. Besides, limited information is available concerning the neonatal safety of embryos with blastomere loss. In the present study, we aimed to investigate the impact of blastomere loss on pregnancy and neonatal outcomes of vitrified/warmed Day3 cleavage-stage embryos in single embryo transfer cycles.

Methods
This retrospective cohort study included all vitrified/warmed D3 cleavage-stage single frozen-thawed embryo transfer (FET) cycles between April 2015 and February 2021. We compared pregnancy and subsequent neonatal outcomes between the intact embryos group and the blastomere loss group in single FET cycles.

Results
A total of 6287 single FET cycles were included in the study, in which 5873 cycles were classified into the intact embryo group and 414 cycles were classified into the blastomere loss group. The outcomes of the blastomere loss group were significantly inferior to those of the intact embryo group, in terms of implantation/biochemical pregnancy/clinical pregnancy/ongoing pregnancy rate and live birth rate per embryo transfer cycle/per clinical pregnancy. Further binary logistic regression confirmed that blastomere loss was negatively associated with live birth. Moreover, the blastomere loss group presented with an elevated early miscarriage rate. The neonatal conditions were broadly similar between the two groups. Additionally, multiple binary logistic regression analysis demonstrated that primary infertility and intracytoplasmic sperm injection (ICSI) were common influencing factors of blastomere loss (aOR 1.447, 95% CI 1.038–2.019, P = 0.029; aOR: 1.388, 95% CI: 1.044–51.846, P = 0.024).

Conclusions
The transfer of vitrified/warmed D3 embryos with blastomere loss is related to impaired embryo developmental potentials and reduced probabilities of conception. Moreover, even if the embryos with blastomere loss have implanted and reached clinical pregnancies, they present with a lower possibility of developing to live birth owing to a higher early miscarriage rate. However, once the embryos with blastomere loss result in a live birth, no adverse neonatal outcomes are observed.
Primary infertility and ICSI were found to be risk factors for blastomere loss.
White matter microstructural and morphometric alterations in autism: implications for intellectual capabilities
Authors: Yeh Chun-Hung , Tseng Rung-Yu , Ni Hsing-Chang , Cocchi Luca , Chang Jung-Chi , Hsu Mei-Yun , Tu En-Nien , Wu Yu-Yu , Chou Tai-Li , Gau Susan Shur-Fen , Lin Hsiang-Yuan 
Published: May 2022

Abstract
Background
Neuroimage literature of autism spectrum disorder (ASD) has a moderate-to-high risk of bias, partially because those combined with intellectual impairment (II) and/or minimally verbal (MV) status are generally ignored. We aimed to provide more comprehensive insights into white matter alterations of ASD, inclusive of individuals with II (ASD-II-Only) or MV expression (ASD-MV).

Methods
Sixty-five participants with ASD (ASD-Whole; 16.6 ± 5.9 years; comprising 34 intellectually able youth, ASD-IA, and 31 intellectually impaired youth, ASD-II, including 24 ASD-II-Only plus 7 ASD-MV) and 38 demographic-matched typically developing controls (TDC; 17.3 ± 5.6 years) were scanned in accelerated diffusion-weighted MRI. Fixel-based analysis was undertaken to investigate the categorical differences in fiber density (FD), fiber cross section (FC), and a combined index (FDC), and brain symptom/cognition associations.

Results
ASD-Whole had reduced FD in the anterior and posterior corpus callosum and left cerebellum Crus I, and smaller FDC in right cerebellum Crus II, compared to TDC. ASD-IA, relative to TDC, had no significant discrepancies, while ASD-II showed almost identical alterations to those from ASD-Whole vs. TDC. ASD-II-Only had greater FD/FDC in the isthmus splenium of callosum than ASD-MV. Autistic severity negatively correlated with FC in right Crus I. Nonverbal full-scale IQ positively correlated with FC/FDC in cerebellum VI. FD/FDC of the right dorsolateral prefrontal cortex showed a diagnosis-by-executive function interaction.

Limitations
We could not preclude the potential effects of age and sex from the ASD cohort, although statistical tests suggested that these factors were not influential. Our results could be confounded by variable psychiatric comorbidities and psychotropic medication uses in our ASD participants recruited from outpatient clinics, which is nevertheless closer to a real-world presentation of ASD. The outcomes related to ASD-MV were considered preliminaries due to the small sample size within this subgroup. Finally, our study design did not include intellectual impairment-only participants without ASD to disentangle the mixture of autistic and intellectual symptoms.

Conclusions
ASD-associated white matter alterations appear driven by individuals with II and potentially further by MV. Results suggest that changes in the corpus callosum and cerebellum are key for psychopathology and cognition associated with ASD. Our work highlights an essential to include understudied subpopulations on the spectrum in research.
Endobronchial ultrasound-guided transbronchial needle aspiration facilitating diagnosis of sarcoidosis in a breast cancer patient with multiple lymphadenopathy: a case report
Authors: Oride Yuka , Koi Yumiko , Sasada Tatsunari , Kajitani Keiko , Ohara Masahiro , Kondo Tomohiro , Daimaru Yutaka , Kawamura Shingo 
Published: May 2022

Abstract
Background
Sarcoidosis is a benign systemic granulomatous disorder of unknown etiology. Cell-mediated immunity disorder is often found in sarcoidosis patients, and an association between malignant tumors and sarcoidosis has been suggested. Sarcoidosis and malignant disease can occur simultaneously or sequentially, leading to misdiagnosis and mistreatment. Sarcoidosis is diagnosed clinically, radiologically, and histologically. We report herein a case of sarcoidosis diagnosed by endobronchial ultrasound-guided transbronchial needle aspiration from the mediastinal lymph nodes of a breast cancer patient.

Case presentation
The patient was a 70-year-old Asian woman who presented with right breast tumor. A 20-mm movable mass was identified in the inferolateral quadrant of the right breast, and mammography revealed a spiculated mass with calcification. Ultrasonography revealed a mass with internal hypoechogenicity, and biopsy revealed estrogen receptor-positive, human epidermal growth factor receptor 2-positive invasive ductal carcinoma. Positron emission tomography/computed tomography showed multiple lymphadenopathy including mediastinal lymph nodes, with fluorodeoxyglucose accumulation in those nodes suggesting breast cancer metastases. Endobronchial ultrasound-guided transbronchial needle aspiration of a mediastinal lymph node revealed noncaseous epithelioid granuloma. Due to a history of uveitis and elevated soluble interleukin 2 receptor, lymphadenopathy due to sarcoidosis and stage IIA breast cancer were diagnosed. Right partial mastectomy and axillary lymph node dissection were performed after preoperative chemotherapy. No exacerbation of sarcoidosis symptoms has been observed during treatment.

Conclusion
We report a case of breast cancer in which sarcoidosis could be diagnosed based on endobronchial ultrasound-guided transbronchial needle aspiration, a history of uveitis, and elevated soluble interleukin 2 receptor despite fluorodeoxyglucose positron emission tomography/computed tomography suggesting multiple lymph node metastases. This report emphasizes the importance of differential diagnosis of lymph node involvements in cancer patients.
A typology of smallholder livestock production systems reflecting the impact of the development of a local milk collection industry: Case study of Fatick region, Senegal
Authors: Habanabakize Ernest , Ba Koki , Corniaux Christian , Cortbaoui Patrick , Vasseur Elsa 
Published: May 2022

AbstractSenegal is a country where millions of livelihoods directly depend on smallholder livestock production activities. Unfortunately, these farmers now face the effects of a changing climate and associated societal responses. In addition, the lack of understanding of the specific farmers’ problems in their respective production systems by assuming homogeneity has led to inappropriate planning of interventions and inefficient utilization of resources. The variations in individual farming systems and local contexts require to understand each system separately to inform decision-makers and relevant stakeholders about specific entries of intervention for farmers based on customized needs. Using a Fatick region case study, different statistical clustering techniques were adopted to develop a livestock typology that reflects the above realities. This typology confirmed the co-existence of three livestock production cluster systems with differences and similarities in their production targets and quantities, market access, animal breeds, and the livestock management system of the animals. The studied region represented a new format of smallholder livestock development, where the implementation of a dairy processor and its milk collection centre created a new dynamic in production activities. Farmers confirmed that the extension services and a consistent market opportunity provided by the dairy processor increased local productivity and offered an alternative source of income but they have also reiterated concerns like the dairy processor’s low milk buying prices which need to be addressed. To harness the growth and the regional opportunities, there is a need to establish robust and continuous collaboration structures between the Government, agro-industries, and producers. These public-private partnerships will help producers lower the cost of inputs and services and increase productivity while boosting local dairy production.
Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks
Authors: Wu Yongbao , Tang Jing , Wen Zhiguo , Zhang Bo , Cao Junting , Zhao Lulu , Guo Zhanbao , Xie Ming , Zhou Zhengkui , Hou Shuisheng 
Published: May 2022

Abstract
Background
Although methionine (Met), the first-limiting dietary amino acid, has crucial roles in growth and regulation of lipid metabolism in ducks, mechanisms underlying are not well understood. Therefore, the objective was to use dietary Met deficiency to investigate the involvement of Met in lipid metabolism and fat accumulation of Pekin ducks.

Methods
A total of 150 male Pekin ducks (15-d-old, 558.5 ± 4.4 g) were allocated into 5 groups (6 replicates with 5 birds each) and fed corn and soybean meal-based diets containing 0.28%, 0.35%, 0.43%, 0.50%, and 0.58% Met, respectively, for 4 weeks. Met-deficient (Met-D, 0.28% Met) and Met-adequate (Met-A, 0.43% Met) groups were selected for subsequent molecular studies. Serum, liver, and abdominal fat samples were collected to assess the genes and proteins involved in lipid metabolism of Pekin ducks and hepatocytes were cultured in vivo for verification.

Results
Dietary Met deficiency caused growth depression and excess fat deposition that were ameliorated by feeding diets with adequate Met. Serum triglyceride and non-esterified fatty acid concentrations increased (P < 0.05), whereas serum concentrations of total cholesterol, low density lipoprotein cholesterol, total protein, and albumin decreased (P < 0.05) in Met-D ducks compared to those in Met-A ducks. Based on hepatic proteomics analyses, dietary Met deficiency suppressed expression of key proteins related to fatty acid transport, fatty acid oxidation, tricarboxylic acid cycle, glycolysis/gluconeogenesis, ketogenesis, and electron transport chain; selected key proteins had similar expression patterns verified by qRT-PCR and Western blotting, which indicated these processes were likely impaired. In vitro verification with hepatocyte models confirmed albumin expression was diminished by Met deficiency. Additionally, in abdominal fat, dietary Met deficiency increased adipocyte diameter and area (P < 0.05), and down-regulated (P < 0.05) of lipolytic genes and proteins, suggesting Met deficiency may suppress lipolysis in adipocyte.

Conclusion
Taken together, these data demonstrated that dietary Met deficiency in Pekin ducks resulted in stunted growth and excess fat deposition, which may be related to suppression of fatty acids transportation and hepatic catabolism.
Traffic and road conditions monitoring system using extracted information from Twitter
Authors: Putra Prabu Kresna , Mahendra Rahmad , Budi Indra 
Published: May 2022

AbstractCongested roads and daily traffic jams cause traffic disturbances. A traffic monitoring system using closed-circuit television (CCTV) has been implemented, but the information gathered is still limited for public use. This research focuses on utilizing Twitter data to monitor traffic and road conditions. Traffic-related information is extracted from social media using text mining approach. The methods include Tweet classification for filtering relevant data, location information extraction, and geocoding in order to convert text-based location into coordinate information that can be deployed into Geographic Information System. We test several supervised classification algorithms in this study, i.e., Naïve Bayes, Random Forest, Logistic Regression, and Support Vector Machine. We experiment with Bag Of Words (BOW) and Term Frequency - Inverse Document Frequency (TF-IDF) as the feature representation. The location information is extracted using Named Entity Recognition (NER) and Part-Of-Speech (POS) Tagger. The geocoding is implemented using the ArcPy library. The best model for Tweet relevance classification is the Logistic Regression classifier with the feature combination of unigram and char n-gram, achieving an F1-score of 93%. The NER-based location extractor obtains an F1-score of 54% with a precision of 96%. The geocoding success rate for extracting the location information is 68%. In addition, a web-based visualization is also implemented in order to display traffic information using the spatial interface.
Health of Pug dogs in the UK: disorder predispositions and protections
Authors: O’Neill Dan G. , Sahota Jaya , Brodbelt Dave C. , Church David B. , Packer Rowena M.A. , Pegram Camilla 
Published: May 2022

AbstractBackgroundPugs are a brachycephalic dog breed that has become phenomenally popular over recent decades. However, there is growing concern about serious health and welfare issues in the breed. To augment the evidence-base on the comparative health of Pugs, this study aimed to compare the odds of common disorders between Pugs and all remaining dogs under primary veterinary care in the UK during 2016.A cross-sectional study design of VetCompass clinical records was used to estimate the one-year (2016) period prevalence for the disorders most commonly diagnosed in Pugs and non-Pugs. Risk factor analysis applied multivariable logistic regression modelling methods to compare the odds of 40 common disorders between Pugs and non-Pugs.ResultsFrom a study population of 905,544 dogs, the analysis included random samples of 4308 Pugs and 21,835 non-Pugs. Pugs were younger (2.36 years, range 0.07–16.24 vs 4.44 years, range 0.01–20.46,p <  0.001) and lighter (8.95 kg, range 5.00–13.60 vs. 14.07 kg, range 1.41–85.00,p <  0.001) than non-Pugs. Pugs had 1.86 (95% confidence interval [CI] 1.72 to 2.01) times the adjusted odds of diagnosis with ≥1 disorder than non-Pugs. Pugs had significantly increased adjusted odds for 23/40 (57.5%) common disorders. These included: brachycephalic obstructive airway syndrome (odds ratio [OR] 53.92; 95% CI 36.22 to 80.28), stenotic nares (OR 51.25; 95% CI 24.93 to 105.37) and corneal ulceration (OR 13.01; 95% CI 10.50 to 16.11). Conversely, Pugs had significantly reduced adjusted odds of 7/40 (17.5%) common disorders compared to non-Pugs. These included: heart murmur (OR 0.23; 95% 0.13 to 0.14), lipoma (OR 0.24; 95% CI 0.11 to 0.55) and aggression (OR 0.31; 95% CI 0.21 to 0.47).ConclusionsThe current study highlights that predispositions outnumber protections between Pugs and non-Pugs for common disorders, suggesting some critical health welfare challenges to overcome for Pugs. Highly differing heath profiles between Pugs and other dogs in the UK suggest that the Pug has diverged substantially from mainstream dog breeds and can no longer be considered as a typical dog from a health perspective.
Stability analysis of multi-serial-link mechanism driven by antagonistic multiarticular artificial muscles
Authors: Ishikawa Yuta , Nabae Hiroyuki , Endo Gen , Suzumori Koichi 
Published: May 2022

AbstractArtificial multiarticular musculoskeletal systems consisting of serially connected links driven by monoarticular and multiarticular muscles, which are often inspired by vertebrates, enable robots to elicit dynamic, elegant, and flexible movements. However, serial links driven by multiarticular muscles can cause unstable motion (e.g., buckling). The stability of musculoskeletal mechanisms driven by antagonistic multiarticular muscles depends on the muscle configuration, origin/insertion of muscles, spring constants of muscles, contracting force of muscles, and other factors. We analyze the stability of a multi-serial-link mechanism driven by antagonistic multiarticular muscles aiming to avoid buckling and other undesired motions. We theoretically derive the potential energy of the system and the stable condition at the target point, and validate the results through dynamic simulations and experiments. This paper presents the static stability criteria of serially linked robots, which are redundantly driven by monoarticular and multiarticular muscles, resulting in the design and control guidelines for those robots.
Acute necrotizing calculous cholecystitis after treatment with ceftriaxone in an elderly patient: a case report
Authors: Shigemori Tsunehiko , Imoto Ichiro , Inoue Yasuhiro , Nishiwaki Ryo , Sugimasa Natsuko , Hamaguchi Tetsuya , Noji Midori , Takeuchi Kenji , Ito Yoshiyuki , Yasuma Taro , Gabazza Esteban C. , Kato Toshio 
Published: May 2022

Abstract
Background
Ceftriaxone, a third-generation cephalosporin antibiotic with a long plasma half-life, is widely used to treat various infections. The use of ceftriaxone can sometimes induce biliary sludge or stone formation. Although most cases of ceftriaxone-induced pseudolithiasis are asymptomatic or mild and resolve with discontinuation of the drug, we experienced an elderly case of severe acute necrotizing calculous cholecystitis after administration of ceftriaxone.

Case presentation
A 72-year-old male patient was admitted to our hospital because of acute diverticulitis in ascending colon. Ceftriaxone was administered at a dose of 2 g/day for 6 days. Although he recovered after therapy, he was readmitted about 2 weeks later because of severe pain with rebound tenderness in the right upper quadrant. An abdominal imaging study revealed stones and sludge in the gallbladder that were not observed before starting ceftriaxone therapy. Therefore, antibiotic treatment with flomoxef 2 g/day was indicated. However, on the fifth day of readmission, the peritoneal irritation symptoms in the right upper quadrant worsened, and elevated inflammatory response and liver dysfunction were observed. Cholecystectomy was performed based on these findings. The resected inflamed gallbladder showed acute necrotizing cholecystitis with sand granular gallstones. A comparative analysis of the infrared spectroscopic pattern of the composition of gallstones collected during surgery with that of the ceftriaxone powder revealed that both have very similar infrared spectroscopic patterns.

Conclusions
Ceftriaxone-related pseudolithiasis is generally reversible and mainly observed in children. Here, we report a rare case of ceftriaxone-related acute necrotizing cholecystitis in an elderly patient. We confirmed that the stones in the gallbladder are composed of ceftriaxone. The older age, dehydration, fasting, and long-time bed rest during the administration of high-dose ceftriaxone were the potential risk factors for gallstone formation.
Study protocol: investigating the feasibility of a hybrid delivery of home-based cluster set resistance training for individuals previously treated for lung cancer
Authors: Fairman C. M. , Owens O. L. , Kendall K. L. , Steele J. , Latella C. , Jones M. T. , Marcotte L. , Peddle-McIntyre C. M. J. , McDonnell K. K. 
Published: May 2022

Abstract
Background
Symptom burden remains a critical concern for individuals with non-small cell lung cancer (NSCLC) following the completion of treatment. The most common symptom clusters, dyspnea (shortness of breath) and fatigue, can contribute to physical decline, reductions in quality of life, and a higher risk of comorbidities and mortality. Dyspnea is a primary limiter of exercise capacity in individuals with lung cancer, resulting in exercise avoidance and an accelerated physical decline. As such, designing resistance training with cluster sets to mitigate symptoms of dyspnea and fatigue may result in improved exercise tolerance. Thus, maintaining the exercise stimulus via cluster sets, combined with improved tolerance of the exercise, could result in the maintenance of physical function and quality of life. The purpose of this study is to investigate the feasibility and preliminary efficacy of a hybrid-delivery home-based cluster-set resistance training program in individuals with NSCLC.

Methods
Individuals with NSCLC (n = 15), within 12 months of completion of treatment, will be recruited to participate in this single-arm feasibility trial. Participants will complete 8 weeks of home-based resistance training designed to minimize dyspnea and fatigue. The hybrid delivery of the program will include supervised sessions in the participants’ home and virtual supervision via video conferencing. The primary outcome of feasibility will be quantified by recruitment rates, retention, acceptability, and intervention fidelity. Exploratory outcomes (dyspnea, fatigue, quality of life, physical function, and body composition) will be assessed pre- and post-intervention.

Discussion
This study will provide important data on the feasibility of delivering this intervention and inform procedures for a future randomized controlled trial.

Trial registration
Record not yet public