bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonlinear SDOF Analytical Model of Mass-Timber Building with Post-Tensioned Rocking Walls
Authors: ALOISIO ANGELO , Rosso Marco Martino , Huang Da , Iqbal Asif , Fragiacomo Massimo , Pei Shiling 
Published: January 2022

Abstract
The dynamic response of post-tensioned rocking walls in a mass timber building can be reduced to a single-degree-of-freedom (SDOF) model. In this model, the rocking wall panel is simplified as a rigid block, while the base rotation represents the degree of freedom of the entire structure. The paper presents an analytical approach to develop and calibrate this nonlinear model using shake table tests of a full-scale two-story building with CLT rocking walls. The experimental data are used to estimate the parameters of the governing equation using least-squares optimization. The correlation between the obtained parameters and the cumulative dissipated energy led to a nonlinear model with degradation behavior captured. After that, the calibrated model was used to assess the fragility functions of the structure under repetitive seismic events.
Biosynthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from leaf &amp; seed extracts of Malva neglecta Wallr.
Authors: Cheperli Abdol-Majid , Mokaber-Esfahani Majid , Taleghani Akram , bahalkeh Farhad 
Published: January 2022

Abstract
Plant-based nanoparticles have a number of benefits over traditional physicochemical approaches, and they may be used in a variety of medical and biological applications. In recent study, a green synthesis approach for zinc oxide nanoparticles (ZnO NPs) using Malva neglecta Wallr. leaf (MWL) and seed (MWS) aqueous extracts were reported. MWL mediated ZnO NPs (MWL-ZnO-NPs) and MWS mediated ZnO NPs (MWS-ZnO-NPs) were synthesized using an environmentally friendly and simple technique with minimal reaction time and calcination temperature. The structural and chemical characterization of the biosynthesized ZnO NPs was performed using UV-Vis, FTIR, XRD, TGA, TEM, and EDX. Synthesized nanoparticles showed the UV–Visible spectroscopy absorption peak at 372 and 376 nm for MWL and MWS-ZnO-NPs, respectively, which confirm the synthesis of ZnO NPs. Furthermore, the FTIR spectrum indicated bioactive functional groups and metal-oxygen groups. The TEM analysis displayed that two different synthesized ZnO NPs, exhibited a somewhat spherical structure with an average particle size between 40 and 50 nm, regardless of the type of extract. EDX analysis approved the Zn and O in the synthesized ZnO NPs. The XRD results revealed that MWL-ZnO-NPs had a similar crystal structure to MWS-ZnO-NPs. The dose-dependent free radical scavenging capability of zinc oxide nanoparticles was determined using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) test. Furthermore, the antibacterial activity of ZnO NPs was investigated using the paper disc diffusion technique against two clinical strains of Escherichia coli and Staphylococcus aureus using the zone of inhibition and minimum inhibitory concentration (MIC).
Patterns of human social contact and mask wearing in representative high-risk groups in China
Authors: Zheng Bo , Zhu Wenlong , Pan Jinhua , Wang Wei-bing 
Published: January 2022

Abstract
Background: The pandemic of COVID-19 has changed human behavior in areas such as contact patterns and mask-wearing frequency. Exploring human-human contact patterns and mask-wearing habits in high-risk groups is an essential step in fully understanding the transmission of respiratory infection-based diseases. Methods: Delivery workers, medical workers, preschoolers, and students. from Qinghai, Shanghai, and Zhejiang were recruited to complete an online questionnaire that queried general information, logged contacts, and assessed the willingness to wear a mask in different settings. The numbers of contacts across different characteristics were assessed and age-specific contact matrices were established. A generalized additive mixed model was used to analyze the associations between the number of individual contacts and several characteristics. The factors influencing the frequency of mask wearing were evaluated with a logistic regression model.Results: A total of 611,287 contacts were reported by 15,635 participants. The frequency of daily individual contacts averaged 3.14 (95%CI: 3.13-3.15) people per day, while that of group contacts was 37.90 (95%CI: 37.20-38.70). Skin-to-skin contact and long-duration contact were more likely to occur at home or among family members. The contact matrices of students were the most assortative (all contacts q-index = 0.899, 95%CI: 0.894-0.904). Participants with larger household sizes reported having more contacts. A higher household income per capita was significantly associated with a greater number of contacts among preschoolers and students. In each of the public places, the frequency of mask wearing was highest in delivery workers. For preschoolers and students with more contacts, the proportion of those who reported always wearing masks was lower (P<0.05) in schools/workplaces and public transportation than preschoolers and students with less contacts.Conclusions: The rate of mask wearing must be improved among preschoolers and students, considering their susceptibility and lower mask-wearing rates. Contact screening efforts should be concentrated in the home, school, and workplace after an outbreak of an epidemic, as more than 75% of all contacts, on average, will be found in such places. Age-stratified and occupation-specific social contact research in high-risk groups could help inform policy-making decisions during the post-relaxation period of the COVID-19 pandemic.
Transcriptional Level of Cumulus-Associated GJA1, PTX3, PRSS35, and SERPINE2 Genes with Oocytes and Embryonic Development in Water Buffalo
Authors: Jeena Lalit , Singh D.K. , Rahangdale Sandeep , Singh Ajit Pratap , Sarkhel B.C. Chandra 
Published: January 2022

Abstract
Background: In the present study, the potential of different groups of cumulus-oocyte complexes (COC’s) for in-vitro maturation (IVM) and embryonic development was assessed in two groups of COC’s of water buffalo. Further, the association of the expression pattern of cumulus-associated GJA1, PTX3, PRSS35, and SERPINE2 genes and their effects on embryonic development was analyzed. Slaughterhouse-derived buffalo oocytes were graded into group A and B based on surrounding cumulus rings. Out of 1000 ovaries, an equal number of 410 COC’s were taken in both the A and B groups. In-vitro maturation (IVM) was carried out using Slaughterhouse-derived buffalo epididymis. A remarkable degree of cumulus expansion was noticed in group A (92.68%) as compared to group B (81.25%) oocytes. On IVF and embryo culture, group A COC’s produced a significantly higher rate of cleavage and blastocyst (92.682±0.7179% and 42.682±0.9683%) as compared to group B COC’s (85.365±0.7608% and 31.707±0.9688%), respectively. The transcriptional analysis of cumulus-associated GJA1, PTX3, PRSS35, and SERPINE2 genes expression by quantitative Real Time-PCR (qRT-PCR) revealed a significantly higher expression in group A as compared to group B COC’s. Result: It was revealed that oocytes having good cumulus mass had a higher developmental potential. Based on differential gene expression of cumulus-associated genes, different quality of COC’s, and the resultant embryos after IVF, it was concluded that these genes could be used as a marker for predicting the developmental competence of the oocytes. Conclusion: It concluded from the study that morphologically good quality of COC’s had a higher developmental competence. Also, the differential expressions of cumulus-associated genes in cumulus cells and embryos, we can conclude that these genes could be used as marker genes for predicting the developmental competence of buffalo’s oocytes.
Novel TUBB1 Mutation Related To Thyroid Dysgenesis In 289 Chinese Patients
Authors: Wang Fang , Sun Chunhui , Li Miaomiao , Ren Yubao , Wang Fengqi , Wang Yangang , Liu Shiguo 
Published: January 2022

Abstract
Objective: We aimed to study the types and characteristics of TUBB1 mutations in a large Chinese cohort with congenital hypothyroidism (CH) and thyroid dysgenesis (TD). Methods: Mutation of the entire coding region of TUBB1 was analysed by Sanger sequencing in 289 children with CH and TD from China. Functional studies were further used to identify the effect of novel mutations on thyroid cells. Results: Among the 289 children with CH and TD, 4 (1.4%) had a c.952C>T(p.R318W) heterozygous mutation in TUBB1, resulting in a change from tryptophan to arginine at codon 318 of the TUBB1 protein. Functional studies indicated that the p.R318W mutant decreased TUBB1 expression and inhibited cell proliferation in a human thyroid cell line. Conclusions: A novel heterozygous missense mutation of TUBB1 in children with CH and TD was identified first in China, laying the foundations to expand the genotype-phenotype spectrum of TD.
Characteristics and Perioperative Complications of Hip Fracture in the Elderly With Acute Ischemic Stroke: A Cross-Sectional Study
Authors: Zhang Yaqian , Fu Mingming , Guo Junfei , Zhao Yuqi , Wang Zhiqian , Hou Zhiyong 
Published: January 2022

Abstract
Background: Patients with acute ischemic stroke (AIS) after hip fracture in the elderly have worse prognosis. We aimed to describe the characteristics and complications of hip fracture with AIS in the elderly. Methods: This cross-sectional study selected patients with hip fracture (age ≥65 years) from January 2018 to September 2020. The collected data included age, sex, fracture types, comorbidities. In above screened patients, we further collected cerebral infarction related information of AIS patients. Risk factors for AIS after hip fracture were identified.Results: 60 patients (mean age 79.7 years; female 56.7%) occurred AIS after hip fracture in 1577 cases. Among 60 AIS patients, the most common cause of fall was limb weakness or instability(46.7%). The most common infarction type was PACI (70.0%). The majority of these infarction lesions were single (76.7%) and most infarction lesions(65.0%) were located in the left side. 81.7% of AIS patients had mild (NIHSS 0-4) AIS. Older patients with AIS after hip fracture were more frequently complicated by hypertension(73.3%), prior stroke (46.7%), diabetes(35.0%) and were more likely to have hypoproteinemia(68.3%), electrolyte disorders (66.7%), anemia (65.0%), deep vein thrombosis (51.6%), pneumonia (46.6%),cardiac complications (45.0%). Combined with hypertension (OR 2.743, 95%CI 1.471-5.115) and male sex(OR 1.946, 95%CI 1.146-3.304) were independent risk factors of AIS after hip fracture. Conclusions: Older patients combined with hypertension are more likely to have AIS after hip fracture. For these patients, early preventions should be administered. AIS patients after hip fracture are prone to have multiple complications under traumatic stress, and we should enhance the management of these patients to reduce the stress and avoid occurrence of complications.
Fertility costs of cryptic viral infections in a model social insect
Authors: Chapman Abigail , Amiri Esmaeil , Han Bin , McDermott Erin , Rueppell Olav , Tarpy David , Foster Leonard J , McAfee Alison 
Published: January 2022

Abstract
Declining insect populations emphasize the importance of understanding the drivers underlying reductions in insect fitness. Here, we investigated viruses as a threat to social insect reproduction, using honey bees as a model species. We report that in a sample of N = 93 honey bee (Apis mellifera) queens from nine beekeeping operations across a wide geographic range, high levels of natural viral infection are associated with decreased ovary mass. We confirmed this finding in an independent sample of N = 54 queens. Failed (poor quality) queens displayed higher levels of viral infection, reduced sperm viability, smaller ovaries, and altered ovary protein composition compared to healthy queens. We experimentally infected queens with Israeli acute paralysis virus (IAPV) and found that the ovary masses of IAPV-injected queens were significantly smaller than control queens, demonstrating a causal relationship between viral infection and ovary size. Queens injected with IAPV also had significantly lower expression of vitellogenin, the main source of nutrition deposited into developing oocytes, and higher levels of heat-shock proteins (HSPs), which are part of the honey bee’s antiviral response. This work together shows that viral infections occurring naturally in the field are compromising queen reproductive success.
Amlodipine and Carvedilol Exhibits Cardioprotective Effect on Cyclophosphomide Induced Cardiotoxicity in Rats.
Authors: N Akshata , KP Shivalinge Gowda 
Published: January 2022

Abstract
Cardiotoxicity is a well-known side effect of several cytotoxic drugs, especially of the anthracyclines and can lead to long term morbidity. The mechanism of anthracycline induced cardiotoxicity seems to involve the formation of free radicals leading to oxidative stress. This may cause apoptosis of cardiac cells or immunologic reactions.CYP has been recorded to be carditoxic. Amlodipine and carvedilol has been exploited to test its cardioprotective activityvon CYP induced cardiotoxicity. Objective: the aim of this study was assessment of cardioprotective effect of Amlodipine and carvedilol on CYP induced cardiotoxicity in albino wistar rats by measuring the enzymatic, non-enzymatic antioxidant levels, serum enzyme levels and study of ECG alteration. Materials Methods: Albino wistar rats were allotted in to 4 groups (6 rats/group), normal control: (i.p. injection with normal saline), CYP group (200 mg/kg ip), Alodipine and CYP group (Amlo-10 mg/kg oral & CYP- 200 mg/kg ip), carvediol and CYP group (Carve-3mg/kg oral & CYP- 200 mg/kg ip) for 10 days of duration and ECG was measured using power lab software. Results: cardioprotects of amlodipine and carvediol significantly reduced the elevated levels of serum biomarkers like CK, CK-MB, LDH, calcium when compare to CYP induced cardiotoxicity. Amlodipine and carvediol has shown significant increase in the levels of tissue biomarkers such as SOD, GSH, catalase when compare to CYP induced cardiotoxicity. In Histopathological studies, the group treated with amlodipine + CYP and carvediol + CYP has shown intact arrangement of cardiac muscle fibres, intact integrity of myocardial cell membrane, myofibrillar structure with striations and continuity with adjacent myofibrils. Considering improvement in the serum biomarker levels and tissue biomarker levels amlodipine and carvediol showed cardioprotective activity. Finally it concluded that amlodipine and carvediol has cardio protective effect on cyclophosphamide induced rats.
Dynamics of Immune Responses are Inconsistent When Trauma Patients are Grouped by Injury Severity Score and Clinical Outcomes
Authors: Yang Yawen , Wu Che-Hsiung , Tsai Huei-Ting , Chen Ying-Ru , Chang Yu-Ping , Han Yin- Yi , Wu Tiffany E. , Hu Ray-Heng 
Published: January 2022

Abstract
Background: The injury severity score (ISS) is used in daily practice to evaluate the severity of trauma patients; however, the score is not always consistent with the prognosis. After injury, systemic inflammatory response syndrome (SIRS) and compensatory anti-inflammatory response syndrome (CARS) are related to the prognosis of trauma patients. Methods: Patients who admitted to the Trauma Intensive Care Unit (ICU) were eligible. Whole blood samples were collected at admission, and then 6, 12, 24, 48 and 72 hours after admission. Natural killer (NK) cells, lymphocyte subset population and cytokines release were identified using flow cytometry. We grouped patients by their ISS (≤ 25 and > 25 as very severe injury) and ICU stay (≤ 10 days as a short ICU stay and >10 days as a long ICU stay) for evaluation. Results: Fifty-three patients were enrolled. ICU stay but not ISS was close correlated with activity daily living (ADL) at discharge. Patients with a long ICU stay had an immediate increase in NK cells followed by lymphopenia which persisted for 48 hours. Immediate activation of CD8 + T cells and then exhaustion with a higher programmed cell death-1 (PD-1) expression and suppression of CD4 + T cells with a shift to an anti-inflammatory Th2 phenotype were also observed in the patients with a long ICU stay. When the patients were grouped by ISS, the dynamics of immune responses were inconsistent to those when the patients were grouped by ICU stay. Conclusion: Immune responses are associated with the prognosis of trauma patients, however the currently used clinical parameters may not accurately reflect immune responses. Further investigations are needed to identify accurate predictors of prognosis in trauma patients.
Microcystic Meningioma Has A Worse Outcome Than Other WHO Grade 1 Subtypes
Authors: Ren Leihao , Hua Lingyang , Bao Zhongyuan , Deng Jiaojiao , Wang Daijun , Chen Jiawei , Chen Hong , Juratli Tareq A. , Wakimoto Hiroaki , Gong Ye 
Published: January 2022

Abstract
Objective: The aim is to evaluate the clinical, radiological features and long-term outcomes of microcystic meningiomas (MM) in a single neurosurgical center.Methods: A total of 87 consecutive patients underwent surgical resection of MM between 2005 to 2016 were enrolled for analysis. Clinical, pathological, radiological and prognostic information was collected and analyzed; Univariate and multivariate COX analysis was conducted to select factors affected the progression-free survival (PFS). PFS was compared among other Grade 1 subtypes in our center as well.Results: 56 females and 31 males were identified. 12 patients (13.8%) experienced tumor progression. The median PFS was unavailable, and the 5-, 10-, and 15-year PFS rates were 96.9%, 84.0%, and 73.9%, respectively. Peri-tumor brain edema (PTBE) was frequent in MMs (85%). Univariate COX analysis demonstrated that skull base location, STR, and higher Ki-67 were significant negative prognostic factors for PFS (P < 0.05), while tumor location and Ki-67 were independent factors (P < 0.01). MM had a worse prognosis when compared to other WHO Grade 1 subtypes diagnosed in our neurosurgical center during the same time period (P = 0.0087).Conclusions: MM is a rare subtype of grade 1 meningioma, PTBE and reticular enhancement were characteristic in MMs. skull base location and higher Ki-67 labeling index were independent negative prognostic factors. MMs had a shorter PFS than the other WHO Grade 1 subtypes.
Hemostatic Radiotherapy for Gastric Cancer: Relationship Between Diffusion-Weighted Magnetic Resonance Images and Tumor Markers
Authors: Tanaka Osamu , Yagi Nobuaki , Tawada Masahiro , Taniguchi Takuya , Ono Kousei , Nakaya Shuto , Makita Chiyoko , Matsuo Masayuki 
Published: January 2022

Abstract
Introduction: Pretreatment diagnosis by diffusion-weighted magnetic resonance imaging (DW-MRI) is useful to determine the effect of chemotherapy. Here we investigated the relationship among DW-MRI, endoscopy, and tumor markers. Patients: Eight included patients underwent hemostatic radiotherapy (RT) for gastric cancer in this prospective study from 2019 to 2021. The patients completed MRI, endoscopy, and blood tests before RT; MRI, endoscopy, and blood tests 1 month after RT; and MRI and blood tests 3 months after RT. Correlations between changes in apparent diffusion coefficient (ADC) derived from DW-MRI and the tumor marker carcinoembryonic antigen (CEA) were investigated. Results: Univariate analysis of overall survival showed that sex and chemotherapy treatment were statistically significant factors. The CEA values before and 1 month after RT decreased significantly. There was no statistical difference between the CEA value 1 and 3 months after RT. The ADC value before and 1 month after RT increased significantly but not between 1 and 3 months after RT. Comparing the ratio of ADC before RT to 1 (or 3) month(s) after RT with that of CEA before RT to 1 (or 3) month(s) after RT, we found an inverse relationship between the two ratios. Conclusions: Therefore, changes in ADC and CEA are correlated. Additionally, 3 months after RT, the decrease in ADC appeared earlier than the decrease in CEA. ADC may indicate a biological change earlier than CEA, and the ratios of ADC and CEA may be important factors. These aspects warrant further confirmation in a larger sample population.
Bias of the Mock Community Composed of Common Strains Isolated from Clinical Specimens
Authors: Shin Jeong Hwan , Kim Eun-Young , Kim Si Hyun , Yang Jaewook 
Published: January 2022

Abstract
Background: The mock communities (MCs) bias of common species from clinical specimens should be considered when we use a microbiome in the clinical microbiology laboratory. The aims of this study were to MCs using clinically important common species and to investigate the bias of MC use in the clinical laboratory.Results: Five MCs incorporating 32 bacterial strains isolated from clinical specimens were included. We analyzed the diversity of operational taxonomic units (OTU), the relative abundance, and the taxonomic assignment using paired-end sequencing of the 16S rRNA V3−V4 region. The Shannon index revealed the best correlation with the actual number of MC species for diversity. We determined that the OTU with relative abundance (log) of 0.001 is most appropriate to explain the community. The relative abundance was higher in Bacteroidetes and Fusobacteria; however, low relative abundance was shown in Aeromonas caviae, Burkholderia cepacia, Enterobacter cloacae, Pseudomonas aeruginosa, and Clostridioides difficile. It is suggested that the relative abundance was low in gram-positive bacteria and those with 60%−70% GC content but was not related to genome size or 16S rRNA copy number.Conclusions: We investigated the characteristics of MC composed as common clinical isolates and confirmed the bias of MC. These data could be used for microbiome research on clinical specimens.
Clinical utility of circulating pneumoproteins as diagnostic biomarkers in COVID-19: a systematic review and meta-analysis
Authors: Ke Yani , Zhu Yuqing , Hu Jie , Chen Shuaihang , Liu Shan 
Published: January 2022

Abstract
This study explored circulating pneumoproteins in the diagnosis, severity, and prognosis of COVID-19 by meta-analysis. We searched six English databases until December 16, 2021. Standardised mean difference (SMD) and 95% confidence interval (CI) were the overall outcomes. RevMan 5.3, Stata 16, and Meta-DiSc 1.4 were utilised for pooled analyses. Totally, 2902 subjects from 29 studies were included. COVID-19 patients had higher circulating KL-6, SP-D, and SP-A levels (SMD=1.34, 95% CI [0.60, 2.08]; SMD=1.74, 95% CI [0.64, 2.84]; SMD=3.42, 95% CI [1.31, 5.53], respectively) than healthy individuals. Circulating KL-6 levels were lower in survivors than in non-survivors (SMD=-1.09, 95% CI [-1.63, -0.55]). Circulating KL-6, SP-D, and RAGEs levels in mild to moderate patients were significantly lower (SMD=-0.93, 95% CI [-1.22, -0.65]; SMD=-1.67, 95% CI [-2.82, -0.52]; SMD=-1.17, 95% CI [-2.06, -0.28], respectively) than severe patients. Subgroup analysis and meta-regression suggested that age may be responsible for the heterogeneity (P=0.071) when analysing KL-6 in mild to moderate vs. severe patients. The meta-analysis of diagnostic accuracy including KL-6 for severity and mortality, and SP-D for severity demonstrated that they all had limited diagnostic values. Therefore, circulating pneumoproteins (KL-6, SP-D, and RAGEs) may reflect the diagnosis, severity, and prognosis of COVID-19 to some extent.
Trapeznikova O.N. Paradoksy severnogo zemledeliya: Istoriya i geografiya agrolandshaftov lesnoy zony Vostochno-Yevropeyskoy zony ischeznoveniya. M.: GEOS, 2019. 256 p. Review
Authors: Фролов А.А. 
Published: January 2022

рецензия на монографию О.Н. Трапезниковой
review of the monograph by O.N. Trapeznikova