bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the influence of keratinized mucosa on the evolution of non-surgical peri-implant treatment in implants with peri-implantitis
Authors: Fons-Badal Carla , Panadero Rubén Agustín , Labaig-Rueda Carlos , Solá-Ruiz Maria Fernanda , García-Selva Marina , Font Antonio Fons 
Published: May 2022

Abstract
The main objective of this study was to evaluate the response to treatment in implants with peri-implantitis depending on the presence or absence of keratinized mucosa. Materials and Methods: 100 implants with peri-implantitis were treated non-surgically at the Prosthodontics and Occlusion Teaching Unit of the University of Valencia. Records were registered at day zero (recession, bleeding on probing, suppuration, probing depth, bone loss and plaque index), at 1, 3 and 6 months. Results: In both groups a slight increase in recession and disappearance of suppuration took place, and no bone loss was observed during the 6 months. However, both probing depth, bleeding on probing and plaque index, after an initial reduction, increased again in the group without keratinized mucosa (KM). Conclusions: Implants with KM presented better results after non-surgical treatment as well as in their medium-term evolution, although it would be advisable to increase the number of samples in order to achieve greater scientific evidence and standardization in the treatment protocol. Clinical relevance: The role of keratinized mucosa in the development of peri-implantitis has been mentioned in many publications, but less has been emphasized regarding its influence on the success of the treatment of this pathology. The presence of keratinized mucosa has been found to play a determinant role in the evolution of non-surgical treatment of peri-implantitis. The amount of keratinized mucosa should be considered in the treatment of peri-implantitis, as well as when planning connective tissue grafts accompanying the decontamination of implants in the absence of keratinized mucosa.
The association between genetic variation rs2292832 and the processing efficiency of pre-miR-149 affects the risk of breast cancer
Authors: Fakhrezare Farzaneh , Ebrahimi Seyed Omar , Reiisi Somayeh 
Published: May 2022

Abstract
Background: microRNAs (miRNAs) play key roles in regulating cancer development, including breast cancer. Variation in miRNA genes can associate with the risk of cancer by alterations in the miRNA’s processing and maturation. Therefore, human blood samples and breast cancer cell line (MCF7) were analyzed to study any possible association between the genetic variant (rs2292832) in the miR-149 precursor and breast cancer susceptibility. Methods: To study the role of rs2292832 polymorphism in breast cancer, the miR-149 gene variant was genotyped using PCR-RFLP. For evaluating the effect of SNP on function and expression levels of mature miR-149, we inserted pre-miR-149 and flanking region with CC or TT genotype into a pEGFPN1 expression vector, and qPCR was accomplished. Cell survival, proliferation, and migration properties investigated by MTT and wound healing assay. Statistical analysis was carried out for data analysis.Results: T allele in variant rs2292832 is associated with an increased risk of breast cancer. Such association was also obtained in co-dominant (OR=2.37) and dominant (OR=1.90) models. The variant allele led to reduced production of mature miR-149 and resulted in increased cell proliferation and migration of MCF7 cells. Conclusion: These findings suggest that miR-149 suppresses tumor cell proliferation, and the pre-mir-149 polymorphism affects the processing of miR-149, causing an alteration in the abundance of the miRNA mature form, which can regulate tumor progression and metastasis.
Urban Ecology and Biological Studies in Brazilian cities: a systematic review
Authors: Sartori Richieri Antonio , Gomes Abraão Carvalho , Narcizo Amanda , Mata Stella , Cárcamo Anna Thereza Bezerra Mello , Matos Rayanne Moreira Andrade , Correa Angelo Antonio , Santana Mariana Henriques , Vieira Maura Andrade , Rajão Henrique 
Published: May 2022

Abstract
The number of papers focusing on ecological interactions in urban environments has increased in recent years. This review aimed to address the panorama of urban ecology and biological surveys in Brazil. A systematic search was carried out using the Web of Science and Scopus platforms for papers on urban ecology to understand which institutions, authors, themes, cities, biomes, states, and regions have addressed the theme in Brazil to date. A total of 932 articles were found, in 196 scientific journals. Most papers were published between 2010 and 2019. This involved 350 municipalities in the five Brazilian regions, with Curitiba, São Paulo, and Rio de Janeiro being the municipalities with the most papers. São Paulo was the state that presented the most papers, with 23.7% of the total, and the Southeast region was the most representative with 36.6%. The biome with the highest concentration of papers (61.2%) was the Atlantic Forest. A total of 2537 authors were registered, affiliated with a total of 413 institutions from 19 countries. The institutions with the most papers were the Federal University of Paraná and University of São Paulo. The most discussed topic was related to botany (69%), and the most used keyword was “urban afforestation”. The number of papers published was greater in municipalities with higher human development index, number of inhabitants and relative urbanized area. This review revealed the scarcity of studies in low-income areas, and the need for greater incorporation of the social aspect, landscape ecology and ecological interactions in urban ecology.
Subaqueous bioprinting: a novel strategy for fetal membrane repair with 7-axis robot-assisted minimally invasive surgery
Authors: Zhao Wenxiang , Hu Chuxiong , Xu Tao , Lin Shize , Wang Ze , Zhu Yu 
Published: May 2022

Abstract
Premature rupture of membranes (PROM), defined as breakage of the amniotic sac prior to delivery, complicates 2–4% of pregnancies, leads to one-third of preterm births, and increases the risk of perinatal mortality and morbidity in survivors. In vivo bioprinting enables the fabrication of three-dimensional (3D) structures directly for tissue repair or reconstruction in living animals but is challenging to perform in the subaqueous narrow space where fetal membranes (FMs) are located. This study reports a novel strategy consisting of an ultrafast photoresponsive hydrogel (1.5 s) and a 7-axis bioprinting robot to perform subaqueous in vivo bioprinting in a minimally invasive approach. Specially, a hydrogel patch with designed gel rivets is printed subaqueously for the therapeutic needs of PROM, and the patch displays robust tissue adhesion, favorable biocompatibility, mechanical properties resembling native tissues, and an appropriate sealing timescale to prolong pregnancy. Experiments performed in an in vitro uterus model and a mid-gestational rabbit model show a favorable sealing effect for PROM, which validates the potential of our subaqueous bioprinting approach for application. This study not only expands upon in vivo bioprinting techniques but also provides valuable insights for the treatment of PROM and other diseases involving tissue injury in clinical settings.
Multi-target Location Based on Compressed Sensing Direct Position Determination with Missing Information
Authors: Huang Xiangsong , Yuan Jia , Yang Zhigang , Su Runjia 
Published: May 2022

Abstract
It often happens that we cannot acquire all information available to locate targets as obstacles exist between observation stations and targets or there is something wrong with some observation stations. Both cases are called the circumstance of missing information where partial information can be observed, resulting in poor positioning performance of multi-target location. Considering the sparse characteristic and random subsampling of Compressed Sensing (CS) theory, we think of a possibility of applying Compressed Sensing Direct Position Determination (CSDPD) to lessen the impact of missing information on multi-target location. Orthogonal matching pursuit (OMP) algorithm is a kind of greedy algorithm of CS, which can be utilized to lessen the impact of missing information. For better positioning accuracy, an improved OMP algorithm based on DPD (IOMP-DPD) is proposed through a new method of updating index set to lessen the possibility of incomplete location. It confirms by simulations that the improved method yields more accurate positioning results than OMP algorithm and outperforms than the existed MUSIC algorithm based on DPD (MUSIC-DPD), revealed in estimating more target locations in the same positioning conditions and smaller positioning errors when locating the same targets.
Incidental learning of faces during threat: No evidence for increased autonomic arousal to “unrecognized” threat identities
Authors: Schellhaas Sabine , Schmahl Christian , Bublatzky Florian 
Published: May 2022

Abstract
Remembering an unfamiliar person and the contextual conditions of that encounter is important for adaptive future behavior, especially in a potentially dangerous situation. Initiating defensive behavior in the presence of former dangerous circumstances can be crucial. Recent studies showed selective electrocortical processing of faces that were previously seen in a threat context compared to a safety context, however, this was not reflected in conscious recognition performance. Here, we investigated whether previously seen threat-faces, that could not be remembered, were capable to activate defensive psychophysiological response systems. During an encoding phase, 50 participants with low to moderate levels of anxiety (partially inpatients) viewed 40 face pictures with neutral expressions (6s each), without an explicit learning instruction (incidental learning task). Each half of the faces were presented with contextual background colors that signaled either threat-of-shock or safety. In the recognition phase, all old and additional new faces (total of 60) were presented intermixed without context information. Participants had to decide whether a face was new or had previously been presented in a threatening or a safe context. Results show moderate face recognition independent of context conditions. Startle reflex and skin conductance response (SCR) were more pronounced for threat compared to safety during encoding. For SCR, this differentiation was enhanced with higher levels of depression and anxiety. There were no differential startle reflex or SCR effects during recognition. From a clinical perspective, these findings do not support the notion that perceptual biases and physiological arousal directly relate to threat-associated identity recognition deficits in healthy and clinical participants with anxiety and trauma-related disorders.
Rapid Vertical Exchange at Fronts in the Northern Gulf of Mexico
Authors: Qu Lixin , Thomas Leif , Wienkers Aaron , Hetland Robert , Kobashi Daijiro , Taylor John , Hsu Fucent , MacKinnon Jennifer , Shearman R. , Nash Jonathan 
Published: May 2022

Abstract
The Texas-Louisiana Shelf in the Northern Gulf of Mexico is home to the second largest human-caused dead zone in the world. Here, the nutrient-laden, stratified waters of the Mississippi River plume condition hypoxia in a bottom layer during the summer. The plume also generates strong fronts, features of the circulation that are known pathways for the exchange of water between the ocean surface and the deep. Using a combination of high-resolution observations and numerical simulations, we show that the vertical exchange at these fronts can be quite rapid and can lead to the oxygenation of bottom water when the fronts are forced by the summer land-sea breeze. These winds generate strong inertial oscillations, which set up a diurnally-pulsing vertical circulation at the fronts that draws bottom waters up to the surface mixed layer. The simulations suggest that during these “breaths” the rate of oxygenation of the bottom waters is comparable to deoxygenation by the respiration of organic matter over the shelf and hence could play an important role in the evolution of the region’s dead zone.
Identification of genes involved in the effects of hypoxia-inducible factor-2 on articular chondrocytes using bioinformatic analysis and validation in osteoarthritis
Authors: Zheng Jiaxuan , Guo Xiangjun , Zhou Kai , Cao Huiyuan , Fang Yehan , Shi Li , Huang Hui 
Published: May 2022

Abstract
Although over-expression of hypoxia-inducible factor-2 alpha (HIF-2α) can result in cartilage destruction and osteoarthritis (OA) development, the underlying mechanisms remain poorly understood. Here, we investigated the molecular mechanisms in chondrocytes over-expressing HIF-2α. The GeneCloud of Biotechnology Information platform was used to identify differentially expressed genes (DEGs). Using the GEO GSE104794 dataset of control (empty adenovirus, n = 4) and experimental (recombinant adenovirus expressing HIF-2α, n = 4) groups, we performed DEG, Gene Ontology, pathway, pathway network, and gene signal network analyses. Similarly, DEG analysis was performed for the GEO GSE51588 dataset of control (non-OA, n = 4) and experimental (OA, n = 20) groups. Thereafter, intersection of GSE104794 gene signal network analysis and GSE51588 DEG analysis was performed for the key genes, validated by quantitative reverse transcription-polymerase chain reaction. A total of 542 DEGs were identified, among which, the 10 most significant genes in the gene signal network were Nfkb1, Tlr2, Nt5e, Enpp1, Entpd3, Vegfa, Ptgs2, Socs3, Fos, and Epas1. The key genes in OA were LUM, ENTPD3, SMPD3, FGFR3, GPX3, IRAK3, EREG, HTR2A, TLR2, and CDA. Taken together, we screened key genes that are potentially involved in osteoarthritis, thereby providing a basis for identifying valuable markers for this disease.
Task prioritization modulates alpha, theta and beta EEG dynamics reflecting proactive cognitive control
Authors: Liegel Nathalie , Schneider Daniel , Wascher Edmund , Arnau Stefan 
Published: May 2022

Abstract
Most neuroscientific studies investigating mental effort apply unspecific effort allocation paradigms. In contrast, the present EEG study targets specific effort allocation during task prioritization. Twenty-eight participants performed a cued number classification task during the retention interval of a working memory task including retrospective cues. One of two possible number classifications was done per trial. Each trial started with a cue indicating which of the two tasks would be more important in the upcoming trial. Subjects were told to engage in both tasks, but to concentrate on the important one. Feedback given at the end of each trial was calculated based on task performance, with scores obtained from the relevant task being tripled.Participants performed significantly better in either task when it was important compared to when not. Task prioritization modulates theta, alpha and beta oscillations, predominantly during task preparation. Multivariate pattern analysis revealed that the exact type of the two possible number classifications was decodable, however, decoding accuracy did not depend on task importance. Hemispheric alpha power asymmetries indicating attentional orienting between working memory representations also did not depend on task importance. The findings suggest that task prioritization primarily affects proactive cognitive control on a superordinate level.
Ethanol Intoxication-Related Metabolic and Biochemical Disturbances in Adolescents: A Matched Case- Control Study
Authors: Hanalioglu Damla , Ozkocer Cansu , Ozalp Esra Can , Dikmen Zeliha Gunnur , Pinar Asli , Teksam Ozlem 
Published: May 2022

Abstract
Background: Alcohol is the most widely consumed substance by adolescents. However, only a few studies investigated metabolic and biochemical changes related with acute ethanol intoxication (AEI) with conflicting results. In this case-matched study, we aimed to investigate clinical and laboratory characteristics of the adolescents with AEI presented to pediatric emergency department (ED) at a tertiary referral center. Methods: All consecutive adolescents with AEI (serum ethanol level >50 mg/dL) (n=335) and their sex- and age-matched control subjects with undetectable ethanol levels who presented to the pediatric ED between 2006-2019 were included in this case-matched study (a total of 670 patients). Demographic, clinical and laboratory data was collected through electronic health records retrospectively. Results: Mean age was 16.0±1.5 years and M/F ratio was 1.5. Mean serum ethanol level was 156.4±58.4 mg/dl (range 50.8-341.2 mg/dl) in the AEI group. Glasgow coma scores were lower in the AEI group (14 [14-15] vs. 15 [15-15], p<0.001). Laboratory investigations revealed that acidosis (16.3%), hyperlactatemia (60.9%), hypoglycemia (1.7%), hypernatremia (2.2%), hypokalemia (12.3%), hyperchloremia (20.4%), hypocalcemia (13.9%), hypermagnesemia (9.7%), hyperalbuminemia (10.4%) were significantly more common in the AEI group as compared to the control group. Blood pH, lactate, Na+, K+, Ca++, Mg++, albumin, BUN and uric acid levels were correlated with serum ethanol levels. Conclusion: Being one of the largest case-matched study of AEI among adolescents published in the literature, this study shows that acute ethanol intoxication frequently leads to mild to moderate metabolic/biochemical derangements in adolescents. Clinicians should be aware of these findings in the management of AEI.
A Study on CBD and Δ9-THC Analysis and Telemedicine of Korean Hemp Seed Foods Manufactured by Oriental Medicine Method
Authors: Kim Namsoo Peter , Eo Yoonhyeong , Aditya Abhilash , Kim Jihye , Kwon SoonHyup 
Published: May 2022

Abstract
Background

Despite the UN's recommendation to abolish restrictions on Cannabidiol (CBD) in the medical and recreation sectors, detailed regulations worldwide on food-grade CBD & Tetrahydrocannabinol (THC) are not adequately reflected.

Methods

The methods of extracting hemp, which was practiced for thousands of years as detailed in ancient oriental medicine literature, were compared with recent Western cannabis case studies, and the possibility of delivering the extracts through food via telemedicine was evaluated. After decades of research, attempts have been made to use improvised hemp or cannabis for medical and cosmetic purposes with lower CBD and THC content, but micro-components, including CBD and THC analysis of Korean hemp seeds(Cheong-Sam), have yet to be accurately reported.

Results

The CBD and THC contents of Korean hemp seeds were measured within the HPLC 300 measurement range and analyzed by diluting 10 times or more to imitate the projected concentration in food. The CBD/THC content of Korean hemp seeds produced in the Andong Hemp Special Zone (AHSZ) was extracted by either hot water extraction or cold compression, similar to traditional medical literature, and then analyzed thoroughly to examine the applicability of traditional oriental methods to food.

Conclusion

CBD and THC contents were safe at less than 3 ppm when using Korean hemp. In the method extracted from the literature, CBD, Δ 9 -THC, tetrahydrocannabinolic acid (THCA), Cannabidiolic acid (CBDA), Cannabidivarinic acid(CBDVA), Cannabigerolic acid (CBGA), Tetrahydrocannabivarin acid (THCVA) responding to human reaction mechanisms were not detected in foods containing Korean hemp (Cheong-Sam) seed and oil used in this study.
Computational Integrated Smart HFRTB Concrete for Sustainable Constructions
Authors: Bajad Mohankumar 
Published: May 2022

Abstract
When hybrid fibre Reinforced Ternary Blended (HFRTB) concretes were exposed to sustained elevated temperatures for three hours (SET-3hrs), the effect of two cooling regimes (sudden and gradual) was examined. At 100o C intervals, the temperature ranges from 100o C to 1000o C. Traditional concrete is compared to two ternary blended (TB) concretes (Cement + fly ash + GGBFS and Cement + fly ash + silica fume), as well as hybrid fibres (Galvanized iron + polypropylene). The specimens were put through a series of strength and durability tests, with slow cooling outperforming abrupt chilling.
Integrated Computational Materials Engineering With Reliability of Green Concrete (GC) For Environmental Safety Buildings
Authors: Bajad Mohankumar N. 
Published: May 2022

Abstract
The compelling usage of left-over constituents of warm force stations, for example, fly debris and bottom ash (BA) as halfway substitution to cement and fine aggregate (FA) in concrete diminish transfer issues. In this examination, the BA is exploited to supplant the normal stream sand up to 100% and in this manner, it diminishes the practice of waterway sand and reuse of BA in concrete which is eco-friendly and can be called green concrete (GC). The most significant overseeing factor that decides the oldness of the concrete structure is durability. BA is utilized as FA (30%, 60% and 100%) in concrete to lessen its ecological contamination (air, land, and water) and to ration characteristic waterway sand which is misused for development. GC comprising BA is intended for 30 MPa with fixed water to fastener proportion and slump esteem; and is assessed for, compressive strength, and elastic modulus. elongated term drying shrinkage (DS) was assessed for 365 days and an experimental relationship was created to foresee 10 years of DS of GC. Test outcome shows that 30%BA came about high compressive strength and elastic modulus than control blend at 90 days. The DS property of BA came about better and even prevalent execution for long term durability. The test examination additionally infers that GC comprising 30% of BA for FA substitution beats control concrete for the structured strength of 30 MPa at 90 days and anticipated DS for extended term durability provided that 10 years.
Toxic Chemical Pollutants in the U.S. States
Authors: Kagawa Shigemi , Tokito Shohei , Fujii Hidemichi , Lenzen Manfred , Faturay Futu , Okamoto Shunsuke 
Published: May 2022

Abstract
Footprint indicators are used to evaluate chemical substance management. However, determining the impact of chemical restrictions on manufacturing processes and supply chains without a footprint analysis of the entire lifecycle is difficult. Here, we propose a new framework for estimating chemical toxicity footprints utilizing the risk-screening environmental indicators (RSEIs) published by the U.S. Environmental Protection Agency. We conducted an empirical analysis using multi-states input–output data from the U.S. in 2017 to demonstrate the usefulness of the framework, and made policy recommendations based on the obtained results. According to the production-based RSEI scores, Texas, Pennsylvania, Illinois, and Louisiana accounted for 43% of the total human health risk in the U.S. By contrast, California and New York ranked first and second in consumption-based RSEI score (i.e., health risk footprint), respectively; however, the District of Columbia and Alabama ranked first and second in per capita footprint. Three significant risk transfers, accounting for 6% of the total in the U.S. economy, were found: from California to Texas, from New York to Texas, and from Florida to Texas. In conclusion, specific states such as Texas and Louisiana were at considerable risk from major chemical substance emissions triggered by final demands in mega cities such as New York and California. Thus, the federal and state governments should select high priority sectors and states according to the production-based RSEI scores. Relevant taxes can be collected from states based on the consumer’s responsibility. Footprint-based inter-state financial cooperation is crucial in mitigating chemical pollutions in the U.S.