bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emergency Unit Assessment of Seven Tertiary Hospitals in Nepal using the WHO Tool: A cross-sectional study
Authors: Kharel Ramu , Thapa Ghan B. , Voor Tamara , Pant Samriddha R. , Adhikari Samir K. , Bist Bimal S. , Relan Pryanka , Lin Timmy , Lubetkin Derek , Deluca Giovanna , Shilpakar Olita , Shrestha Sanu K. , Pokharel Yagya R. , Paudel Santosh , Thapa Ajay S. , Shakya Yogendra M. , Karki Achyut R. , Dhakal Nishant , Aluisio Adam R. 
Published: November 2022

Abstract
Background
In 2021, the Nepal national emergency care system’s assessment (ECSA) identified 39 activities and 11 facility specific goals to improve care. To support implementation of the ECSA facility-based goals, this pilot study used the World Health Organization’s (WHO) Hospital Emergency Unit Assessment Tool (HEAT) to evaluate key functions of emergency care at tertiary hospitals in Kathmandu, Nepal.
Methods
This cross-sectional study used the standardized HEAT assessment tool. Data on facility characteristics, human resources, clinical services, and signal functions were gathered via key informant interviews conducted by trained study personnel. Seven tertiary referral centers in the Kathmandu valley were selected for pilot evaluation including governmental, academic and private hospitals. Descriptive statistics were generated and comparative analyses were conducted.
Results
All facilities had continuous emergency care services, but differed in the extent of availability of each item surveyed. Academic institutions had the highest rating with greater availability of consulting services and capacity to perform specific signal functions including breathing interventions and sepsis care. Private institutions were found to have the highest infrastructure availability and diagnostic testing capacity. Across all facilities, common barriers included lack of training of key emergency procedures, written protocols, point of care testing, and ancillary patient services.
Conclusion
This pilot assessment demonstrates that current emergency care capacity at representative tertiary referral hospitals in Kathmandu, Nepal is variable with some consistent barriers which preclude meeting the ECSA goals. The results can be used to inform emergency care development within the region studied and demonstrate that the WHO HEAT assessment is feasible and may be instructive in systematically advancing emergency care delivery at the national level if implemented more broadly.
Analysis of the difference between early-bolting and non-bolting roots of Angelica dahurica based on transcriptome sequencing
Authors: Wu Ping , Wang Xiaoyu , Guo Junxai , Zhang Songli , Li Qingmiao , Fang Qingmao , Luo Bin , Wang Hongsu , He Weijing 
Published: November 2022

Abstract
Angelica dahurica（Fisch. ex Hoffm.）Benth.et Hook.f.var.formosana（Boiss.）Shan et Yuan (A. dahurica) is a famous traditional Chinese medicine commonly used in clinic, with anti-inflammatory, analgesic, anti-tumor, antibacterial, whitening and other pharmacological effects. However, early bolting of A. dahurica compromises the quality of the roots and hence is a major limitation for yield of medicinal materials. To date, the molecular factors of early bolting occurrenc and its effect on the growth of A. dahurica are unexplored. Hence, we commenced a transcriptome study by using the Illumina NovaSeq 6000 at two developmental types i.e. early-bolting and non-bolting (normal) roots of A. dahurica. Overall, 2,185 up and 1,414 down-regulated genes were obtained. A large number of identified transcripts were related to genes involved in early bolting. Gene ontology analysis highlighted differentially expressed genes that play a key role in various pathways. These pathways were mainly linked to cellular, molecular and biological processes. The morphological characteristics and coumarins content were also changed signifcantly in the early bolting roots of A. dahurica. This study lays a path towards understanding the transcriptomic control of early bolting in A. dahurica, which could be further applied to enhance the medicinal value.
Scientific Reports Shore Power Load Forecasting based on a Novel Transformer Method with Feature Selection
Authors: Li Weihao , Zuo Youhong , Su Tao , Zhao Weiyou , Ma Xiaoxue , Cui Guangkai , Wu Jiabin 
Published: November 2022

Abstract
Shipping plays an important role in transporting goods, but it also brings air pollution such as nitrogen and sulfur compounds. Meanwhile, shore power can provide daily work power for ships calling at ports, and it can reduce the pollution of the port. Load forecasting of shore power plays an important role in power decision-making. In this work, we proposed a load forecasting model based on the Transformer and the conditional generation. The Firefly Algorithm (FA) was designed to extract the represented condition features. The proposed shore power transformer (SP-T) method adopts the probability distribution of the target load as the prediction results. The two sub-models, SP-T-1 and SP-T-2, were used to predict the Gaussian distribution parameters μ and σ, respectively. It allowed decision-makers to set the confidence level to obtain the range of predicted values according to the actual situation. To evaluate the proposed model, we used 328 power load data (about 18568.4 hours) of different ships that berth at Zhenjiang Port with shore power. The experimental results showed that SP-T can effectively predict the load data of shore power, with an average P50RMSE was 46.997 and an average P90RMSE was 34.822.
TRIM36 regulates neuroendocrine differentiation of prostate cancer via HK2 ubiquitination and GPx4 deficiency
Authors: Zhao Xusong , Zhou Tianren , Wang Yuhao , Bao Meilingling , Ni Chenbo , Ding Lei , Sun Shengjie , Dong Huiyu , Li Jie , liang Chao 
Published: November 2022

Abstract
Neuroendocrine prostate cancer (NEPC) arises from transdifferentiated prostate adenocarcinoma following androgen deprivation therapy (ADT), which belongs to the most lethal subtype of castration-resistant prostate cancer (PCa). ADT paradoxically promoted the incidence of NEPC, with a mechanism awaiting to be clarified. Trigred motif 36 (TRIM36), a member of the trim protein family, participates in a variety of cellular processes. Our previous experimental results have confirmed that TRIM36 was highly expressed in PCa and inhibited the invasion and proliferation of PCa. In this study, we found that TRIM36 was associated with the neuroendocrine differentiation (NED) phenotype. TRIM36 was found to inhibit the NEPC of the PCa cell lines LNCaP and PC3. The autophagic degradation of HK2 (hexokinase 2), a crucial glycolytic enzyme catalyzing the conversion of glucose to glucose-6-phosphate, was found to be involved in the regulation of glycolysis by autophagy. TRIM36 specifically bound to HK2 and inhibited the glycolysis of PCa cells through lysine 48 (lys48)-mediated ubiquitination of HK2. The degradation of HK2 reduced the expression of GPX4, a protein that inhibits ferroptosis, a novel form of nonapoptotic programmed cell death. Ferroptosis enhancement can further inhibit the NED of PCa cells. In conclusion, TRIM36 can ubiquitinate HK2, inhibit glycolysis in PCa cells, inhibit GPX4 activity, promote ferroptosis of PCa cells, and then inhibit their NED. Our study may design new strategies to retard NED and treat NEPC.
One-Pot Synthesis of Hyperbranched Polymers via Visible Light Regulated Switchable Catalysis
Authors: Zhu Shuaishuai , Zhao Maoji , Zhou Hongru , Wen Yingfeng , Wang Yong , Liao Yonggui , Zhou Xingping , Xie Xiaolin 
Published: November 2022

AbstractSwitchable catalysis promises exceptional efficiency in synthesizing polymers with ever-increasing structural complexity. However, current achievements in such attempts are limited to constructing linear block copolymers. Here we report a visible light regulated switchable catalytic system capable of synthesizing hyperbranched polymers in a one-pot/two-stage procedure with commercial glycidyl acrylate (GA) as a heterofunctional monomer. Using (salen)CoIIICl (1) as the catalyst, the ring-opening reaction under a carbon monoxide atmosphere occurs with high regioselectivity (> 99% at the methylene position), providing an alkoxycarbonyl cobalt acrylate intermediate (2a) during the first stage. Upon exposure to light, the reaction enters the second stage, wherein2aserves as a polymerizable initiator for organometallic-mediated radical self-condensing vinyl polymerization (OMR-SCVP). Given the organocobalt chain-end functionality of the resulting hyperbranched poly(glycidyl acrylate) (hb-PGA), a further chain extension process gives access to a core-shell copolymer with brush-on-hyperbranched arm architecture. Notably, the post-modification with 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) affords a metal-freehb-PGA that simultaneously improves the toughness and glass transition temperature of epoxy thermosets, while maintaining their storage modulus.
Osteoclastogenesis of human peripheral blood, bone marrow, and cord blood monocytes
Authors: Vuoti Ella , Lehenkari Petri , Tuukkanen Juha , Glumoff Virpi , Kylmäoja Elina 
Published: November 2022

AbstractOsteoclasts are multinucleated bone resorbing cells that can be differentiated from human monocytesin vitro. There are few studies comparing osteoclastogenesis of different monocyte sources. We compared monocytes from human bone marrow (BM), peripheral blood (PB), and umbilical cord blood (CB) and their osteoclastogenic potential by culturing them with osteoclastogenic agents. We also cultured cells without growth factors, as umbilical cord blood monocytes have been reported to be able to fuse spontaneously into osteoclasts. After culture with RANKL and M-CSF, all types of cell cultures developed TRACP -positive multinuclear cells that were able to form resorption pits on human bone slices. Only occasional multinuclear cells and small infrequent resorbed areas could be found in PB and CB-derived cultures without growth factors. BM-derived cells formed greater resorption areas than PB- and CB-derived monocytes. The greatest monocyte population in BM samples were intermediate (CD14++CD16+) and in PB and CB classical monocytes (76.3% and 54.4%, respectively). Our data demonstrates that osteoclast precursor origin can effect the osteoclast function and that osteoclasts can be differentiated from CB samples. Therefore CB can be used in osteoclastin vitrostudies.
Assessing sustainability of transport system through index: A state-of-the-art review
Authors: Ayadi Hana , Benaissa Mounir , Hamani Nadia , Kermad Lyes 
Published: November 2022

Abstract
The transport is one of the most important sectors that affect the economic development. However, its sustainability may be disturbed due to the remarkable increase in the number of vehicles in city centers and its bad consequences, hence the importance of assessing the transport system and the performance of its different components. Therefore, several evaluation methods were introduced to help decision-makers assess sustainability and many indicators were developed to track the progress of sustainable transport system. This work provides a literature review about the existing approaches used to assess transport sustainability through composite indicators. It aims at analyzing the trends in the existing literature, identifying gaps in evaluating sustainability and suggesting future research perspectives. A total of 47 studies conducted in the period 2002–2022 are examined. The obtained results show that few researchers focused on freight transportand most researchers considered only traditional sustainability dimensions. In addition, the performed analysis demonstrates that the use of different methods of normalization, weighting and aggregation influences the result given by composite indicator. Finally, a set of recommendations for precise and correct sustainability assessment is presented in order to develop future research.
Peripheral Sensory Stimulation of the Hand in the Treatment of Stroke. A Preliminary Study of Safety and Effectiveness
Authors: Nussbaum Eric S. , Janjua Tariq M. , Lowary Jodi , Defillo Archie , Myers Mark T. , Nussbaum Leslie A. 
Published: November 2022

Abstract
Background
Ischemic stroke impacts roughly 700,000 patients per year in the United States, yet there are limited options to improve recovery for individuals with a completed stroke and associated neurological deficit. We describe a preliminary clinical trial of a novel non-invasive device that provides peripheral somatosensory stimulation using intermittent jets of air to the affected hand in patients with ischemic stroke.
Methods
The safety and short-term impact of the use of a non-invasive device providing peripheral somatosensory stimulation were investigated in four patients after mild to moderate stroke. Perfusion MR imaging was performed with the device off and then on in each patient. Serial evaluations of the NIH stroke scale score, Glasgow Outcome Score, and Barthel Index were performed in each case.
Results
All patients tolerated treatment with the device. No complications related to the use of the device were encountered. A non-blinded neuroradiologist interpreted the MR imaging as demonstrating improved perfusion in three of four cases. All patients demonstrated neurological improvement over the course of treatment.
Conclusions
It has been suggested that peripheral somatosensory stimulation may improve recovery from stroke. We investigated the use of a novel non-invasive device that delivers intermittent jets of air to the affected forearm and hand. In this small, preliminary series, treatment was well-tolerated in all cases. A larger trial properly empowered to demonstrate clinical benefit may be warranted.
A Numerical investigation of Two Critical Conditions for the First Steady Bifurcation of Natural Convection Flow of Air Enclosed within Rectangular Cavities
Authors: zhu wenfang , wang xiaohua 
Published: November 2022

Abstract
For a given fluid, the aspect ratio and the corresponding Rayleigh number are the two critical conditions for bifurcations of natural convection flow enclosed in rectangular cavities. As none given references has directly shown the critical conditions of the natural convection flow of air encolsed in rectangular cavities, the second-order Euler-Taylor-Galerkin (ETG) finite element method developed by the authors was used for computing the critical aspect ratios and the critical Ra for the first steady bifurcation of natural convection flow of air within rectangular cavities (vertical, horizontal, and square cavities). The bisection method was used for finding the critical ratio of W/H or H/W accordingly. As the results indicate, the critical W/H for horizontal rectangular cavities is about 2.12, and the critical H/W for vertical rectangular cavities is about 3.62. Based on the investigation of the critical aspect ratios, the first steady bifurcations for the cavities having the critical aspect ratios were then computed numerically by using different Ra. It was found that for the rectangular cavities having critical aspect ratios, the first natural air convection flow bifurcation was that of an inverse pitchfork bifurcation with two given vortices converged into one core cell while the Ra was larger than the critical value. The critical Ra for a critical vertical rectangular cavity was about 434.5 when using the bisection method, and the critical Ra for a horizontal rectangular cavity was about 55.5. For a better understanding of the variation of the first steady bifurcation modes in rectangular cavities with W/H (H/W), the natural convection flow of air within a square cavity computed to investigate the first steady bifurcation in it and to find the critical Ra. It was found for the square cavity that the first steady bifurcation was a pitchfork bifurcation with one core cell broken into two vortices while Ra>31314. The critical Ra lay between (31313, 31314). It can be deduced that the aspect ratio is one key factor for the flow structure evolution of natural convection flows enclosed within the cavities. Moreover, the bifurcation diagram and the mechanism of eddy emergence for such flow needs to be numerically computed in a systematic manner.
Synonymous Sites for Accessibility around MicroRNA Binding Sites in Bacterial Spot and Speck Disease Resistance Genes of Tomato
Authors: Sophiarani Yengkhom , Chakraborty Supriyo 
Published: November 2022

Abstract
The major causes of mass tomato infections in both covered and open ground are agents of bacterial spot and bacterial speck diseases. MicroRNAs (miRNAs) are 16–21 nucleotides in length, non-coding RNAs that inhibit translation and trigger mRNA degradation. MiRNAs play a significant part in plant resistance to abiotic and biotic stresses by mediating gene regulation via post-transcriptional RNA silencing. In this study, we analyzed a collection of bacterial resistance genes of tomato and their binding sites for tomato miRNAs and Pseudomonas syringe pv. tomato miRNAs. Our study found that two genes, bacterial spot disease resistance gene (Bs4) and bacterial speck disease resistance gene (Prf), have a 7mer-m8 perfect seed match with miRNAs. Bs4 was targeted by one tomato miRNA (sly-miR9470-3p) and three Pseudomonas syringe pv. tomato miRNAs (PSTJ4_3p_27246, PSTJ4_3p_27246 and PSTJ4_3p_27246). Again, Prf gene was found to be targeted by two tomato miRNAs viz., sly-miR9469-5p and sly-miR9474-3p. The accessibility of the miRNA-target site and its flanking regions, as well as the relationship between relative synonymous codon usage (RSCU) and tRNAs were compared. Strong access to miRNA targeting regions and decreased rate of translations suggested that miRNAs might be efficient in binding to their particular targets. We also found the existence of rare codons, which suggests that it could enhance miRNA targeting even more. The codon usage pattern analysis of the two genes revealed that both were AT-rich (Bs4 = 63.2%; Prf = 60.8%). We found a low codon usage bias in both genes, suggesting that selective restriction might regulate them. The silencing property of miRNAs would allow researchers to discover the involvement of plant miRNAs in pathogen invasion. However, the efficient validation of direct targets of miRNAs is an urgent need that might be highly beneficial in enhancing plant resistance to multiple pathogenic diseases.
Peripheral tissue perfusion and associated factors in individuals with type 2 diabetes mellitus: cross-sectional study
Authors: Faria Valéria , Fernandes Juliana Simões de Alencar , Cunha Tulio Ericles de Oliveira , Pussieldi Guilherme de Azambuja , Pereira Danielle Aparecida Gomes 
Published: November 2022

Abstract

Background: Early recognition of peripheral tissue perfusion deficits can minimize secondary complications of peripheral arterial disease in individuals with diabetes.
Aims: To compare peripheral tissue perfusion of the lower limb between individuals with type 2 diabetes (T2DM) without apparent vascular disease and apparently healthy individuals and to evaluate factors associated with peripheral tissue perfusion.
Methods: 62 individuals participated in this study: 31 with T2DM and 31 apparently healthy individuals. In both groups, anthropometric measurements and physical activity levels were evaluated. Peripheral tissue perfusion was analyzed using near-infrared spectroscopy during the arterial occlusion maneuver and the Incremental Shuttle Walking Test.
Results: The tissue oxygen saturation (StO2) level during progressive effort was lower in the T2DM group (T2DM, 58.74 [56.27–61.74]; healthy, 62.15 [59.09–66.49]; p = 0.005). There were significant correlations between the StO2 level during progressive effort and physical activity level (p < 0.0001; r = -0.681), total body fat percentage (p = 0.001; r = 0.590), segmental body fat percentage (p < 0.0001; r = 0.616), total skeletal muscle mass (SMM; p < 0.0001; r = -0.628), and segmental SMM (p = 0.001; r = -0.592).
Conclusions: There is a change in tissue perfusion of the lower limb in individuals with T2DM and simple measures can help understand tissue microcirculation in this group, allowing clinical conduct to anticipate vascular complications.
Investigation of dynamic mixedness characteristics of a transverse acoustically excited turbulent jet by high-repetition-rate acetone tracer Planar laser-induced fluorescence technique
Authors: Yan Bo , Sun Yongchao , Sun Mingbo , Li Fan , Wu Ge , Wan Minggang , Tian Yifu , Li Qinyuan , Chen Shuang , Zhu Jiajian 
Published: November 2022

Abstract

The dynamic mixedness characteristics of a bluff-body stabilized turbulent jet under transverse acoustic excitations are investigated using high-repetition-rate acetone planar laser-induced fluorescence (PLIF) at 7 kHz and multipoint scanning hot-wire measurements. Acetone mixedness imaging is made for the turbulent jet to assess the interaction between the turbulent jet and the imposed transverse acoustic excitations at a driving frequency of 50 Hz. The high-repetition-rate acetone PLIF images show that the acetone mixedness distribution swings left and right frequently under the transverse acoustic excitation, and the deflection angle can reach about 6°. The mixedness area of a turbulent jet flow can also be increased by 13.3% when excited by a transverse acoustic wave. Meanwhile, the sequence of acetone instantaneous PLIF images illustrates how the wrinkled edges are generated when acoustic excitations are imposed. The curvature of the acetone PLIF interface shows that the portion of large curvatures increases to 1.6 times after applying an acoustic wave of 123 dB. Multipoint hot-wire measurements further stress that the turbulence intensity at the transverse acoustic excitation of 123 dB increases to be about 1.3 times the natural turbulence. The proper orthogonal decomposition results show that the large and small scales of the jet wrinkles both increase with the sound pressure level. RANS transient simulation also implies that a stronger turbulent kinetic energy distribution and distorted velocity streamlines can be achieved inside the turbulent jet due to the transverse acoustic excitation. They can further lead to increased mixing between the turbulent jet and the surrounding air.
Viticulture in Oltenia Region (Romania) in the New Climatic Context
Authors: Vlăduț Alina Ștefania , Licurici Mihaela , Burada Cristina Doina 
Published: November 2022

Abstract

As climate is one of the determining factors for grape production and quality, the viticultural potential of a region is greatly affected by climate changes, particularly temperature increase. Oltenia is one of the traditional Romanian winegrowing regions that is presently undergoing a progressive warming trend, which may require adaptation measures in the near future. The analysis is based on monthly values of temperature (mean, maximum and minimum), precipitation amount and sunshine duration from 10 meteorological stations located within the study area. The data cover the period 1961–2021, the modifications of the main climatic parameters and specific bioclimatic indices being analysed for the entire period and on two distinct sub-periods (1961–1990 and 1991–2021). The obtained results reveal an increase of the heliothermal resources in the region and a certain stability of the precipitation amounts during the last three decades. Thus, both Winkler and Huglin indices emphasize a northward shift of the area corresponding to quality red wines (about 60 km), which means also a shift of the quality white wines in areas considered without great potential so far. Trends detected in the climatic parameters and bioclimatic indices emphasize potential problems for viticulture in Oltenia, mainly in its southern and southwestern part, where climate suitability for quality wines is under pressure on the background of increasing temperature and reduced precipitation amounts during the growing season.