bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive assessment of four whole blood stabilizers for flow‐cytometric analysis of leukocyte populations
Authors: Nguyen Ngoc Anh , Huang Xiaobo , Cabrera Luz E. , Pekkarinen Pirkka T. , Nowlan Kirsten , Strandin Tomas , Kantele Anu , Vapalahti Olli , Heinonen Santtu , Kekäläinen Eliisa 
Published: October 2022

AbstractThough cryopreservation of cell fractions is widely used in flow cytometry studies, whole blood cryopreservation is more challenging due to the presence of erythrocytes and effects of fixatives commonly used for preservation. Here, we evaluated and compared head‐to‐head the performance of four commercial whole blood cryopreservation kits; (1) Cytodelics, (2) Stable‐Lyse V2 and Stable‐Store V2 (SLSS‐V2), (3) Proteomic stabilizer (PROT‐1), and (4) Transfix. We found that PROT‐1, Transfix, and Cytodelics maintained the distribution of major leukocyte subsets—granulocytes, T cells, natural killer cells, and B cells, on a comparable level to unpreserved samples, despite the attenuation of fluorescence intensities in flow cytometric assays. Moreover, these three stabilizers also maintained the activated phenotypes of neutrophils upon stimulation with N‐formylmethionyl‐leucyl‐phenylalanine and lipopolysaccharides. The upregulation of adhesion molecules (CD11b), Fc receptors (CD16), and granule proteins (CD66b), as well as the shedding of surface L‐selectin (CD62L), was conserved most efficiently in PROT‐1 and Cytodelics when compared to samples only treated with erythrocyte lysing. However, none of the stabilizers provided a reliable detection of CCR7 for accurate quantification of T cell maturation stages. We also evaluated the performance of Cytodelics in longitudinal clinical samples obtained from acute COVID‐19 patients, where it allowed reliable detection of lymphopenia and granulocyte expansion. These results support the feasibility of whole blood cryopreservation for immunophenotyping by flow cytometry, particularly in longitudinal studies. In conclusion, the performance of different stabilizers is variable and therefore the choice of stabilizers should depend on cell type of interest, as well as antibody clones and experimental design of each study.
Highly Flexible Graphene‐Film‐Based Rectenna for Wireless Energy Harvesting
Authors: Zhang Jingwei , Wang Yuchao , Song Rongguo , Kou Zongkui , He Daping 
Published: October 2022

Herein, we report the design, fabrication, and performance of two wireless energy harvesting devices based on highly flexible graphene macroscopic films (FGMFs). We first demonstrate that benefiting from the high conductivity of up to 1 × 106 S m−1 and good resistive stability of FGMFs even under extensive bending, the FGMFs‐based rectifying circuit (GRC) exhibits good flexibility and RF‐to‐DC efficiency of 53% at 2.1 GHz. Moreover, we further expand the application of FGMFs to a flexible wideband monopole rectenna and a 2.45 GHz wearable rectenna for harvesting wireless energy. The wideband rectenna at various bending conditions produces a maximum conversion efficiency of 52%, 46%, and 44% at the 5th Generation (5G) 2.1 GHz, Industrial Long‐Term Evolution (LTE) 2.3 GHz, and Scientific Medical (ISM) 2.45 GHz, respectively. A 2.45 GHz GRC is optimized and integrated with an AMC‐backed wearable antenna. The proposed 2.45 GHz wearable rectenna shows a maximum conversion efficiency of 55.7%. All the results indicate that the highly flexible graphene‐film‐based rectennas have great potential as a wireless power supplier for smart Internet of Things (IoT) applications.
Seismic vulnerability of reinforced concrete buildings with discontinuity in columns
Authors: Gwalani Payal , Singh Yogendra , Varum Humberto 
Published: October 2022

AbstractIrregular reinforced concrete (RC) buildings constitute a significant portion of the existing housing stock. A common type of irregularity is in the form of discontinuity in the vertical framing elements, which can exacerbate their seismic vulnerability. The design guidelines available in seismic design codes essentially cater to only regular buildings, and the safety of such buildings, even when the other guidelines of the codes are followed, is doubtful. This article evaluates the vulnerability of RC frame buildings with discontinuity in columns designed for modern seismic codes, in the form of seismic collapse capacity, collapse resistance against maximum earthquake demand level, and failure mechanism. The adequacy and limitations of the provisions of the seismic design codes are evaluated for such buildings. Analysis results show that the sequential analysis of buildings considering the construction staged effects, considerably affects the design and hence the collapse failure mechanism of even low‐ and mid‐rise buildings. The results also underline the importance of strong column–weak beam design in the seismic performance of the floating column buildings. The vertical component of ground motion is also observed to be relatively more crucial in floating column buildings.
Postdiagnosis dietary factors, supplement use and breast cancer prognosis: Global Cancer Update Programme (CUP Global) systematic literature review and meta‐analysis
Authors: Becerra‐Tomás Nerea , Balducci Katia , Abar Leila , Aune Dagfinn , Cariolou Margarita , Greenwood Darren C. , Markozannes Georgios , Nanu Neesha , Vieira Rita , Giovannucci Edward L. , Gunter Marc J. , Jackson Alan A. , Kampman Ellen , Lund Vivien , Allen Kate , Brockton Nigel T. , Croker Helen , Katsikioti Daphne , McGinley‐Gieser Deirdre , Mitrou Panagiota , Wiseman Martin , Cross Amanda J. , Riboli Elio , Clinton Steven K. , McTiernan Anne , Norat Teresa , Tsilidis Konstantinos K. , Chan Doris S. M. 
Published: October 2022

AbstractLittle is known about how diet might influence breast cancer prognosis. The current systematic reviews and meta‐analyses summarise the evidence on postdiagnosis dietary factors and breast cancer outcomes from randomised controlled trials and longitudinal observational studies. PubMed and Embase were searched through 31st October 2021. Random‐effects linear dose‐response meta‐analysis was conducted when at least three studies with sufficient information were available. The quality of the evidence was evaluated by an independent Expert Panel. We identified 108 publications. No meta‐analysis was conducted for dietary patterns, vegetables, wholegrains, fish, meat, and supplements due to few studies, often with insufficient data. Meta‐analysis was only possible for all‐cause mortality with dairy, isoflavone, carbohydrate, dietary fibre, alcohol intake and serum 25‐hydroxyvitamin D (25(OH)D), and for breast cancer‐specific mortality with fruit, dairy, carbohydrate, protein, dietary fat, fibre, alcohol intake and serum 25(OH)D. The results, with few exceptions, were generally null. There was limited‐suggestive evidence that predefined dietary patterns may reduce the risk of all‐cause and other causes of death; that isoflavone intake reduces the risk of all‐cause mortality (relative risk (RR) per 2 mg/day: 0.96, 95% confidence interval (CI): 0.92‐1.02), breast cancer‐specific mortality (RR for high vs low: 0.83, 95% CI: 0.64‐1.07), and recurrence (RR for high vs low: 0.75, 95% CI: 0.61‐0.92); that dietary fibre intake decreases all‐cause mortality (RR per 10 g/day: 0.87, 95% CI: 0.80‐0.94); and that serum 25(OH)D is inversely associated with all‐cause and breast cancer‐specific mortality (RR per 10 nmol/L: 0.93, 95% CI: 0.89‐0.97 and 0.94, 95% CI: 0.90‐0.99, respectively). The remaining associations were graded as limited‐no conclusion.
American Academy of Periodontology best evidence consensus statement on the use of biologics in clinical practice
Authors: Avila‐Ortiz Gustavo , Ambruster Jeanne , Barootchi Shayan , Chambrone Leandro , Chen Chia‐Yu , Dixon Douglas R. , Geisinger Maria L. , Giannobile William V. , Goss Katie , Gunsolley John C. , Heard Rick H. , Kim David M. , Mandelaris George A. , Monje Alberto , Nevins Marc L. , Palaiologou‐Gallis Angela , Rosen Paul S. , Scheyer E. Todd , Suarez‐Lopez del Amo Fernando , Tavelli Lorenzo , Velasquez Diego , Wang Hom‐Lay , Mealey Brian L. 
Published: October 2022

AbstractA biologic is a therapeutic agent with biological activity that is administered to achieve an enhanced regenerative or reparative effect. The use of biologics has progressively become a core component of contemporary periodontal practice. However, some questions remain about their safety, indications, and effectiveness in specific clinical scenarios. Given their availability for routine clinical use and the existing amount of related evidence, the goal of this American Academy of Periodontology (AAP) best evidence consensus (BEC) was to provide a state‐of‐the‐art, evidence‐based perspective on the therapeutic application of autologous blood‐derived products (ABPs), enamel matrix derivative (EMD), recombinant human platelet‐derived growth factor BB (rhPDGF‐BB), and recombinant human bone morphogenetic protein 2 (rhBMP‐2). A panel of experts with extensive knowledge on the science and clinical application of biologics was convened. Three systematic reviews covering the areas of periodontal plastic surgery, treatment of infrabony defects, and alveolar ridge preservation/reconstruction and implant site development were conducted a priori and provided the foundation for the deliberations. The expert panel debated the merits of published data and exchanged experiential information to formulate evidence‐based consensus statements and recommendations for clinical practice and future research. Based on an analysis of the current evidence and expert opinion, the panel concluded that the appropriate use of biologics in periodontal practice is generally safe and provides added benefits to conventional treatment approaches. However, therapeutic benefits and risks range based on the specific biologics used as well as patient‐related local and systemic factors. Given the limited evidence available for some indications (e.g., gingival augmentation therapy, alveolar ridge preservation/reconstruction, and implant site development), future clinical studies that can expand the knowledge base on the clinical use of biologics in periodontal practice are warranted.
Fibrotic and inflammatory characteristics of epidural fat adjacent to the ossification area in patients with ossification of the ligament flavum
Authors: Dou Xinyu , Mao Tianli , Ma Yunlong , Jia Fei , Liu Yu , Liu Xiaoguang 
Published: October 2022

AbstractObjectivesTo observe histological and inflammatory characteristics of epidural fat (EF) adjacent to the ossification area in patients with ossification of the thoracic ligament flavum (TOLF) and provide a preliminary research basis for investigating the impact of the EF on OLF.MethodsSamples of EF and autologous subcutaneous adipose tissue (SCAT) from TOLF patients (n = 26) and non‐TOLF patients (n = 23) were harvested during posterior thoracic spine surgery. Adipocyte size and fibrosis were measured by histology. Vascularization and inflammatory cell infiltration were evaluated by immunohistochemical staining. Adipogenesis‐related genes were assessed by real‐time quantitative PCR. Conditioned media from cultured EF were evaluated via enzyme‐linked immunosorbent assay to detect the secretion of inflammatory cytokines, including interleukin‐6 (IL‐6), tumor necrosis factor (TNF‐α), and leptin. The phosphorylated STAT3 protein level in ligament flavum (LF) was examined using Western blot.ResultsAdipocytes size in EF was similar between in the TOLF and non‐TOLF groups, but significantly smaller than that from autologous SCAT. Adipogenesis‐related mRNA expression in EF was lower than that in SCAT. More fibrosis and vascularization were found in TOLF than in non‐TOLF. EF in the TOLF group exhibited more macrophages and B lymphocytes infiltrated. The levels of cytokines such as IL‐6, TNF‐α, and leptin secreted by EF were significantly higher in the TOLF group than non‐TOLF. The level of phosphorylated STAT3 in LF was significantly upregulated in the TOLF group.ConclusionsMorphologically, EF adjacent to the ossification area is smaller and more uniform than autologous SCAT, exhibiting a characteristic similarity to visceral fat. EF in the TOLF group shows a more fibrotic, vascularized, and inflammatory phenotype, which secretes multiple cytokines. The phosphorylated STAT3 protein was significantly upregulated in the TOLF group. Whether these properties of EF directly affect the process of OLF needs to be further explored.
5‐Aminolevulinic Acid‐Mediated Photodynamic Therapy Improves Vocal Fold Wound Healing in Rats
Authors: Zhang Chi , Pang Wenting , Gong Ting , Jiang Jack J. , Silverman Matthew , Li Gelin 
Published: October 2022

ObjectiveStudies showed that photodynamic therapy (PDT) might be able to prevent vocal fold scar formation when treating laryngeal lesions. We aim to investigate if PDT improves vocal wound healing and reduces scar formation in both prophylactic and remodeling procedures performed in vivo.Study DesignIn vivo.MethodsVocal fold stripping was performed in Sprague–Dawley rats. PDT was performed with intraperitoneal injection of 100 mg/kg 5‐Aminolevulinic Acid (5‐ALA) and 635 nm laser irradiation of 20, 40, and 60 J/cm2. PDT was performed immediately after surgery to study the prophylactic effect and 4 weeks after surgery to study the remodeling effect. Gene expression was evaluated with real‐time PCR at 1 week after PDT. Histologic evaluations were performed 12 weeks after PDT, including hematoxylin–eosin, Masson, Alcian blue staining, and immunohistochemical staining of collagen I and III.ResultsPDT induced similar effects on the vocal fold wound healing outcomes in both prophylactic and remodeling procedures. Expression of MMP8, MMP13, HAS2, and TGFβ1 was significantly elevated. Histologic evaluation revealed significantly increased thickness, decreased density of collagen, and increased deposition of hyaluronic acid in the lamina propria. Immunohistochemistry also revealed better distribution and reduced density of collagen I and III. The most obvious changes were seen in the 60 J/cm2 PDT group.ConclusionPDT could significantly improve vocal wound healing by providing both prophylactic effects and remodeling effects. It may be a minimally invasive treatment for vocal fold lesions with slight vocal scarring, and may be used to treat acute or chronic vocal injury to reduce vocal scarring.Level of EvidenceN/A Laryngoscope, 133:1943–1951, 2023
SRv6‐based Time‐Sensitive Networks (TSN) with low‐overhead rerouting
Authors: Nandha Kumar Gagan , Katsalis Kostas , Papadimitriou Panagiotis , Pop Paul , Carle Georg 
Published: October 2022

AbstractTime‐Sensitive Networks (TSN) aims at providing a solid underpinning for the support of application connectivity demands across a wide spectrum of use cases and operational environments, such as industrial automation and automotive networks. However, handling network updates in TSN entails additional challenges, stemming from the need to perform both flow rerouting and TSN schedule reconfiguration. To address this issue, we propose a software‐defined network (SDN)‐based approach for low‐overhead TSN network updates, exploiting segment routing over IPv6 (SRv6) for path control. To this end, we introduce the concept of TSN subgraphs in order to quickly reschedule the flows traversing the problematic area and propose a TSN‐aware routing heuristic to minimize the convergence time. We further describe the control plane implementation and its integration into Mininet, which empowers us to conduct a wide range of performance tests. Our evaluation results indicate that our approach yields faster recovery and reduces significantly the number of required reconfigurations upon failures, at the expense of a small SRv6 encoding/decoding overhead.
Ternary structure design based on hydrogen bonding for transparent and flame retardant PMMA with good mechanical properties
Authors: Xie Wang , Liu Yuan , Yu Mingming , Wang Qi 
Published: October 2022

AbstractThe synthesis of intrinsic flame retardant copolymer by copolymerization with reactive flame retardants is the most potential method to prepare transparent and flame retardant poly (methyl methacrylate) (PMMA) at present，but the main challenge of this method is that the copolymer usually has poor mechanical properties and heat resistance. In this work, the hydrogen bond enhancement strategy is adopted, and the flame retardant PMMA with excellent comprehensive properties is obtained by ternary copolymerization with methyl methacrylate (MMA) as matrix unit, diethyl (methacryloyloxymethyl) phosphonate (DEP) as flame retardant unit and methacrylamide (MAA) as hydrogen bond unit. Due to the formation of intermolecular hydrogen bond via MAA unit, the storage modulus, flexural strength and impact strength of the terpolymer containing 15 mol% MAA are 48%, 19%, and 24% higher than those of the copolymer of MMA and DEP, and its hardness, glass transition temperature and load thermal deformation temperature (increased by 7°C) are also superior. Moreover, owing to the gas‐phase dilution and charring flame retardancy of MAA unit, the terpolymer shows increased limiting oxygen index (24.3%) and UL94 rating (V‐1). This work not only provides a promising flame retardant PMMA for practical application, but also offers a new strategy to design flame retardant polymers with good mechanical properties.
Synthesis and chemoselective crosslinking of functionalized polyesters from bio‐based epoxides and cyclic anhydrides
Authors: Haslewood Marcell N. D. , Farmer Thomas J. , North Michael 
Published: October 2022

AbstractBiomass derivable epichlorohydrin undergoes ring‐opening copolymerization with either succinic or itaconic anhydride (both biosourcable) under standard laboratory conditions using metal‐based or an entirely organic initiator to form highly functionalized, low molecular weight polyesters. The linear polyesters obtained from epichlorohydrin and itaconic anhydride contain both alkyl chlorides and α,β‐unsaturated esters and can be crosslinked to form insoluble resins by treatment with di‐ to tetra‐functional amines and thiols. Crosslinking with amines predominately occurs through the alkyl chlorides whilst crosslinking with thiols occurs through the α,β‐unsaturated esters allowing the synthesis of insoluble polyester derived resins with complementary and controllable functionalities.
Simultaneous fault‐tolerant control and disturbance rejection for systems with fault in polynomial form
Authors: Jiao Yu , Pang Guochen , Mou Xiaojian , Zhang Ancai , Qiu Jianlong , Cao Jinde 
Published: October 2022

AbstractThis article studies the simultaneous fault‐tolerant control and disturbance rejection for systems with fault in polynomial form. The unknown external disturbance is considered to be caused by the external source system, and the fault can be expressed as a polynomial function of time. Combining the fault observer and the disturbance observer, a controller is designed, which can not only diagnose the fault, but also improve the anti‐disturbance performance. Finally, a numerical example is given to verify the validity of the results.
Bet on innovation, not Environmental, Social and Governance metrics, to lead the Net Zero transition
Authors: Madden Bartley J. 
Published: October 2022

AbstractThe conventional Net Zero perspective with its emphasis on Environmental, Social and Governance (ESG) metrics represents linear cause‐and‐effect thinking. That is, implementation of the metrics will then change company behaviour with the eventual effect of a successful Net Zero transition. Different perspectives are presented, rooted in systems thinking. Numerous company examples explain why innovation, not ESG metrics, will be the prime mover in achieving Net Zero. The crux of the argument for systems thinking is that a company like Honeywell, currently given an ‘F’ ESG score, is delivering stellar innovations that will enable its customers to significantly reduce their greenhouse gas emissions. As to behaviour, boards of directors should demand new information from management with particular attention to returns on capital versus the cost of capital under scenarios that include a price (tax) on carbon. The integration of corporate finance principles with systems thinking will improve decision‐making and facilitate an efficient Net Zero transition.
Establishment and validation of a risk prediction model for postpartum stress urinary incontinence based on pelvic floor ultrasound and clinical data
Authors: Liu Wei , Qian Linxue 
Published: October 2022

Abstract
Introduction and hypothesis
This study aimed to establish a risk prediction model for postpartum stress urinary incontinence (SUI) based on pelvic floor ultrasound measurement data and certain clinical data.

Methods
Singleton pregnant women aged ≥ 18 years who underwent delivery were selected. All participants were followed up to determine the symptoms of SUI, and pregnancy-related data were collected at the time of registration. Pelvic floor ultrasound was performed at 6–12 weeks postpartum to obtain ultrasonic measurement data. Logistic regression analysis was used to select predictors and establish a nomogram to predict the risk of postpartum SUI. Area under the ROC curve (AUC) values and calibration curves were used for discrimination and calibration, respectively. Finally, external verification of the model was carried out.

Results
A total of 255 participants were included in the analysis, comprising 105 in the postpartum SUI group and 150 in the non-SUI group. Logistic regression analysis identified age, parity, vaginal delivery, bladder neck descent (BND), and angle of internal urethral orifice funnel as risk factors for postpartum SUI (all P < 0.05).

Conclusions
We constructed a prediction model for postpartum SUI based on pelvic floor ultrasound measurement data and certain clinical data. In clinical practice, this convenient and reliable tool can provide a basis for formulation of treatment strategies for patients with postpartum SUI.
Recent advances in cutaneous lymphoma—implications for current and future classifications
Authors: Goodlad JR , Cerroni L , Swerdlow SH 
Published: October 2022

Abstract
The Revised European-American Classification of mature lymphoid neoplasms published in 1994 and the 2001, 2008 and 2016 WHO classifications that followed, were the product of international collaboration and consensus amongst haematopathologists, geneticists, molecular scientists and clinicians. Primary cutaneous lymphomas were fully incorporated into this process following the publication of the WHO-EORTC classification of cutaneous lymphomas in 2005. The definition, diagnostic criteria and recommended studies for primary cutaneous lymphoma continue to be refined. The 2022 International Consensus Classification represents the most recent update and an overview of all the main entities presenting primarily in the skin, together with the major changes in classification, are summarized herein. Primary cutaneous marginal zone lymphoma is segregated from other extranodal marginal zone lymphomas of mucosa-associated lymphoid tissue (MALT lymphoma) and downgraded to a lymphoproliferative disorder in line with its markedly indolent behaviour. In addition, two subtypes are recognised, based largely but not exclusively on whether they are heavy chain class-switched or IgM positive. Similarly, in keeping with a trend to greater conservatism, primary cutaneous acral CD8 positive T cell lymphoma is now also classified as a lymphoproliferative disorder. In addition, significant new insights into the biology of primary cutaneous lymphoma have also recently been forthcoming and will be presented. These studies have enhanced our knowledge of genetic, epigenetic and transcriptional changes in this group of diseases. They not only identify potential targets for novel therapies, but also raise as yet unanswered questions as to how we categorise cutaneous lymphomas, particularly with respect to relationships with similar lymphomas at extracutaneous sites.