bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterizing restricted and unusual interests in autistic youth
Authors: Spackman Emily , Smillie Luke D. , Frazier Thomas W. , Hardan Antonio Y. , Alvares Gail A. , Whitehouse Andrew , Uljarević Mirko 
Published: December 2022

AbstractA broad range of interests characterized by unusual content and/or intensity, labeled as circumscribed interests (CI), are a core diagnostic feature of autism. Recent evidence suggests that a distinction can be drawn between interests that, although characterized by unusually high intensity and/or inflexibility, are otherwise common in terms of their content (e.g., an interest in movies or animals), labeled as restricted interests (RI), and interests that are generally not salient outside of autism (e.g., an interest in traffic lights or categorization), labeled as unusual interests (UI). The current study aimed to further characterize RI and UI by exploring their association with age, sex, IQ, and social motivation, as well as to examine differences in the adaptive benefits and negative impacts of these two subdomains. Parents of 1892 autistic children and adolescents (Mage = 10.82, SDage = 4.14; 420 females) completed an online survey including the Dimensional Assessment of Restricted and Repetitive Behaviors and the Social Communication Questionnaire. Both RI and UI were found to be highly frequent. Sex‐based differences were observed in the content, but not intensity, of CI such that females were more likely to show interests with a social component. Finally, RI and UI showed distinct patterns of association with age, sex, IQ, and social motivation, as well as metrics of adaptive benefits and negative impacts. Findings afford a more nuanced understanding of sex‐based differences in CI and, crucially, provide preliminary evidence that RI and UI represent distinct constructs that should be studied independently in future research.
Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway
Authors: Xu Qixuan , Yao Yizhou , Ni Haishun , Gu Jinrong , Wang Xuchao , Jiang Linhua , Wang Bin , Zhu Xinguo 
Published: December 2022

AbstractBackgroundGastric cancer (GC) ranks fourth among the causes of death from malignant tumors in the world. Studies have implicated the dysregulation of circRNAs with GC. However, the relationship between hsa‐circ‐0052001 and GC is unclear.MethodsIn our current study, we assessed the expression levels of hsa‐circ‐0052001 in GC cells and tissues using quantitative real‐time PCR (qPCR). The role of hsa‐circ‐0052001 expression on the proliferation and invasion of GC cells was assessed using in vitro experiments. The role of hsa‐circ‐0052001 on the proliferation of GC cells was also analyzed using in vivo models. The pathways downstream of hsa‐circ‐0052001 were identified using bioinformatics analyses, western blot (WB) assays, and qRT‐PCR.ResultsWe found that compared with normal gastric mucosa epithelial cells and adjacent paracancer tissues, hsa‐circ‐0052001 was overexpressed in GC cells and tissues. Also, the hsa‐circ‐0052001 level was linked to patient clinicopathological characteristics of GC. Cell proliferation and metastatic ability were inhibited in gastric cancer cells when hsa‐circ‐0052001 was knocked down in vitro and cancer growth in vivo. Mechanistically, hsa‐circ‐0052001 promoted the carcinogenesis of GC cells via the MAPK signal pathway.ConclusionHsa‐circ‐0052001 functions as a tumor gene in promoting the progression of GC through MAPK pathway, which has provided a promising target for patients with GC.
Transcriptional upregulation of CXCL13 is correlated with a favorable response to immune checkpoint inhibitors in lung adenocarcinoma
Authors: Park Sehhoon , Cha Hongui , Kim Hong Sook , Lee Boram , Kim Soyeon , Kim Tae Min , Jung Hyu Ae , Sun Jong‐Mu , Ahn Jin Seok , Ahn Myung‐Ju , Park Keunchil , Park Woong‐Yang , Lee Se‐Hoon 
Published: December 2022

AbstractBackgroundThe chemokine CXCL13 is known to influence local anti‐tumor immunity by recruiting immune cells and forming tertiary lymphoid structures (TLS). It has been hypothesized that TLS, led by the expression of CXCL13, could be a predictive or prognostic biomarker for immunotherapy. We investigated the predictive value of CXCL13 to immune checkpoint inhibitors (ICI) in lung adenocarcinoma.MethodsWe constructed an exploratory dataset (n = 63) and a validation dataset (n = 57) in metastatic lung adenocarcinoma patients treated with ICI. Based on the clinical response, the difference in gene expression profile, including CXCL13, was evaluated.ResultsFrom the exploratory dataset, CXCL13 expression was significantly upregulated in the ICI responders (p = 0.002). Survival analysis using a cut‐off value of the median expression value of CXCL13 showed prolonged progression‐free survival (PFS) (p = 0.004) and overall survival (OS) (p = 0.007). CXCL13 expression was correlated with other immune response genes, such as GZMA, CD8A, IFNG, PRF1, TLS‐related gene sets and its receptor, CXCR5. Notably, subgroup analyses based on CXCL13 expression and CD8A showed that CXCL13‐upregulated patients demonstrated comparably prolonged survival regardless of CD8A expression. In the validation dataset, CXCL13 upregulation also demonstrated a significant prolongation of both PFS (p = 0.050) and OS (p = 0.026).ConclusionWe observed that CXCL13 upregulation is correlated to better ICI response in lung adenocarcinoma. Our results support that CXCL13 could be an important chemokine in shaping the immunoactive tumor microenvironment which affects the anti‐tumor effect of ICI.
An integrative review of the person‐centred and experiential therapy literature on delivering individual video counselling and psychotherapy
Authors: Rodgers Brian , Tudor Keith , Sutherland Andrew 
Published: December 2022

AbstractBased on an integrative review of the relevant literature in and on person‐centred and experiential (PCE) therapy, this study identifies and discusses the findings of 13 articles on the delivery of individual video counselling and psychotherapy. Six overarching themes were identified: the discrepancy of the experiences of clients and practitioners; the possibility of good‐quality PCE therapy (psychotherapy and/or counselling); “doing” PCE therapy online; “being” online; the role of technology; and implications for training and practice. It is suggested that all these have implications for ongoing practice and education/training, and need to be considered by practitioners, educators/trainers, and professional bodies.
Photoclick and Release: Co‐activation of Carbon Monoxide and a Fluorescent Self‐reporter, COS or Sulfonamide with Fast Kinetics
Authors: Liu Miao , Wang Yuhan , Yan Zhicheng , Yang Jiabin , Wu Yongyou , Ding Dawei , Ji Xingyue 
Published: December 2022

AbstractBioorthogonal prodrugs with both fast reaction kinetics and multiple outputs are highly desirable but are only found sporadically. Herein, we report a novel photoclick‐and‐release strategy for the co‐activation of carbon monoxide and a self‐reporter, carbonyl sulfide, or sulfonamide with fast reaction kinetics (k: 1.4–22.6 M−1 s−1). Such a photoclick‐and‐release strategy was successfully applied in live cells to deliver carbon monoxide and a fluorescent self‐reporter, both of which exhibited pronounced antiproliferative activity against 4T1 cancer cells. It is conceivable that this photoclick‐and‐release strategy could find applications in other fields, in which a controlled bond cleavage is preferred.
In situ X‐ray Absorption Spectroscopy in Homogeneous Conditions Reveals Interactions Between CO2 and a Doubly and Triply Reduced Iron(III) Porphyrin, then Leading to Catalysis
Authors: Mendoza Daniela , Dong Si‐Thanh , Kostopoulos Nikolaos , Pinty Victor , Rivada‐Wheelaghan Orestes , Anxolabéhère‐Mallart Elodie , Robert Marc , Lassalle‐Kaiser Benedikt 
Published: December 2022

AbstractIron porphyrins are attractive catalysts for the electrochemical reduction of carbon dioxide (CO2), owing to their high activity and selectivity while being tunable through ligand functionalization. Iron tetraphenyl porphyrin (FeTPP) is the simplest of them, and its catalytic behavior toward CO2 has been studied for decades. Although kinetic information is available, spectroscopic signatures are lacking to describe intermediate species along the catalytic cycle. In situ UV‐Visible and X‐ray absorption near edge spectroscopy (XANES) were used to monitor the local and electronic structure of FeTPP homogeneously dissolved in dimethyl formamide (DMF) under reductive potentials. The Fe(III) starting species was identified, together with its one, two and three electron‐reduced counterparts under both argon and CO2 atmospheres. Under argon, the second and third reductions lead to species with electronic density shared between the metal and the porphyrin backbone. In the presence of CO2 and with a low amount of protons, the doubly and triply reduced species interact with CO2 at the metallic site. In light of these results, an electronic structure for a key intermediate along the catalytic cycle of the CO2‐to‐CO reduction reaction is proposed.
Chemoselective Methionine Labelling of Recombinant Trastuzumab Shows High In Vitro and In Vivo Tumour Targeting
Authors: Hashad Rania A. , Jap Edwina , Casey Joanne L. , Candace Ho Y. T. , Wright Alexander , Thalmann Claudia , Sleeman Mark , Lupton David W. , Hagemeyer Christoph E. , Cryle Max J. , Robert Remy , Alt Karen 
Published: December 2022

AbstractA highly effective 2‐step system for site‐specific antibody modification and conjugation of the monoclonal antibody Herceptin (commercially available under Trastuzumab) in a cysteine‐independent manner was used to generate labelled antibodies for in vivo imaging. The first step contains redox‐activated chemical tagging (ReACT) of thioethers via engineered methionine residues to introduce specific alkyne moieties, thereby offering a novel easy way to fundamentally change the process of antibody bioconjugation. The second step involves modification of the introduced alkyne via azide‐alkyne cycloaddition ′click′ conjugation. The versatility of this 2‐step approach is demonstrated here by the selective incorporation of a fluorescent dye but can also be applied to a wide variety of different conjugation partners depending on the desired application in a facile manner. Methionine‐modified antibodies were characterised in vitro, and the diagnostic potential of the most promising variant was further analysed in an in vivo xenograft animal model using a fluorescence imaging modality. This study demonstrates how methionine‐mediated antibody conjugation offers an orthogonal and versatile route to the generation of tailored antibody conjugates with in vivo applicability.
Yolk‐shell Co3O4@Fe3O4/C Nanocomposites as a Heterogeneous Fenton‐like Catalyst for Organic Dye Removal
Authors: Yang Ruixia , Peng Qiaohong , Ahmed Adeel , Gao Fengyuan , Yu Bing , Shen Youqing , Cong Hailin 
Published: December 2022

AbstractThe yolk‐shell Co3O4@Fe3O4/C nanocomposites with Co3O4 as the core, Fe3O4/C as the shell, and a cavity structure were synthesized by the hard template method. The physical and chemical properties of the composites were characterized by SEM, TEM, XRD, TGA, XPS, BET, and VSM. The specific surface area of yolk‐shell Co3O4@Fe3O4/C nanocomposites is 175.9 m2 g−1, showing superparamagnetic properties. The yolk‐shell Co3O4@Fe3O4/C nanocomposites were used as heterogeneous Fenton catalysts to activate peroxymonosulfate (PMS) to degrade MB, which showed high catalytic degradation performance. The degradation rate of MB reached 100 % within 30 min under the circumstances of the yolk‐shell Co3O4@Fe3O4/C nanocomposites dosage of 0.1 g L−1, the PMS dosage of 1.0 g L−1, the initial MB concentration of 100 mg L−1, an initial pH of 5.5, and a temperature of 30±2 °C. The enhanced catalytic performance of the yolk‐shell Co3O4@Fe3O4/C nanocomposites can be attributed to the synergistic effect of the two catalytically active materials and the middle cavity. The effects of different operating parameters and co‐existing anion species on MB degradation were also investigated. Electron paramagnetic resonance (EPR) analysis and quenching experiments confirmed that the formation of SO4⋅− in the yolk‐shell Co3O4@Fe3O4/C/PMS system contributes to MB degradation. In addition, yolk‐shell Co3O4@Fe3O4/C nanocomposites can be easily separated from the pollutant solution under the action of an external magnetic field, and the degradation rate of MB can still reach 98 % after five cycles, indicating that it has good stability and reusability and has broad application prospects in the field of water purification.
RGD Peptidomimetic MMAE‐Conjugate Addressing Integrin αVβ3‐Expressing Cells with High Targeting Index**
Authors: Paulus Jannik , Nachtigall Beate , Meyer Peter , Sewald Norbert 
Published: December 2022

AbstractSmall molecule‐drug conjugates (SMDCs) mimicking the RGD sequence (‐Arg‐Gly‐Asp‐) with a non‐peptide moiety require a pharmacophore‐independent attachment site. A library of 36 sulfonamide‐modified RGD mimetics with nM to pM affinity for integrin αVβ3 was synthesized and analysed via DAD mapping. The best structure of the conjugable RGD mimetic was used and a linker was attached to an aromatic ring by Negishi cross‐coupling. The product retained high affinity and selectivity for integrin αVβ3. The conjugable RGD mimetic was then attached to an enzymatically cleavable GKGEVA linker equipped with a self‐immolative PABC and the antimitotic drug monomethyl auristatin E (MMAE). The resulting SMDC preferred binding to integrin αVβ3 over α5β1 in a ratio of 1 : 4519 (ELISA) and showed selectivity for αVβ3‐positive WM115 cells over αVβ3‐negative M21‐L cells in the in vitro cell adhesion assay as well as in cell viability assays with a targeting index of 134 (M21‐L/WM115).
Green‐Emissive Copper Nanocluster with Aggregation‐Enhanced Emission for Selective Detection of Al3+
Authors: Zhang Yi , Lu Zhangdi , Feng Anrui , Lam Jacky W. Y. , Wang Zhenguang , Shi Yu‐e , Tang Ben Zhong 
Published: December 2022

AbstractSelective detection of Al3+ is of great significance both for the benefit of human health and environmental safety considerations. In this work, a sensitive and selective fluorescence assay for Al3+ was proposed based on the green‐emissive Cu nanoclusters (Cu NCs). Different from the commonly reported works, the green emissive Cu NCs showed dual emission bands at 450 and 510 nm, attributed to the reaction product between polyvinyl pyrrolidone and ascorbic acid and the Cu core, respectively. Al3+ could induce the aggregation of Cu NCs by forming covalent bonds, which results in the enhancement of photoluminescence intensity. This enhancement phenomenon is rather selective to Al3+, which endows the detection in real samples. These results provide new insights for the fluorescence mechanisms of metal NCs, which also provided a functional luminescent material for various applications, such as chemical sensing, bioimaging and photoelectric devices.
A Copper‐Catalyzed Interrupted Domino Reaction for the Synthesis of Fused Triazolyl Benzothiadiazine‐1‐oxides
Authors: Hommelsheim Renè , Bausch Sandra , Selvakumar Arjuna , Amer Mostafa M. , Truong  Khai‐Nghi , Rissanen Kari , Bolm Carsten 
Published: December 2022

AbstractCopper(I)‐catalyzed domino reactions of 2‐azido sulfoximines with 1‐iodoalkynes yield fused triazolyl‐containing benzothiadiazine‐1‐oxides. The protocol features the synthesis of two fused heterocyclic rings in one step with good to excellent yields and a broad functional group tolerance. Detailed mechanistic investigations indicate that a copper π‐complex initiates a cycloaddition and oxidative C−N coupling reaction sequence. The results suggest an interrupted domino process involving an iodinated triazole as a key intermediate.
Band Structure Engineering of Single Pt Atoms on Fe−TiO2 for Enhanced Photocatalytic Performance
Authors: Ma Xujun , Wang Donghui , Wu Jianan , Zhao Bin , Chen Feng 
Published: December 2022

AbstractSingle atomic site catalysts display the maximal atom‐utilization efficiency, unique structural properties, and remarkable enhancements on catalytic activity. Herein, single Pt atoms loaded Fe−TiO2 catalysts were prepared. Fe3+ doping leads to the formation of oxygen vacancies and improve the interaction between TiO2 and Pt. Single Pt atoms are thus anchored and effectively modify the local energy band structure of TiO2. The optimized local band structures improve the intrinsic photoexcitation of Pt/Fe−TiO2, promote the separation of photogenerated carriers, and extend the lifetime of photogenerated carriers. Meanwhile, the electrons transfer from the excited dyes to the conduction band edge of Pt/Fe−TiO2 is also facilitated due to the shift‐down of the conduction band edge. Therefore, with the increase of the Pt content (till up to 0.6 wt%), the photocatalytic performance of Pt/ Fe−TiO2 with the confined single Pt atoms is significantly boosted in either the intrinsic or the sensitized photocatalytic process.
Demographic effects of a megafire on a declining prairie grouse in the mixed‐grass prairie
Authors: Parker Nicholas J. , Sullins Daniel S. , Haukos David A. , Fricke Kent A. , Hagen Christian A. , Ahlers Adam A. 
Published: December 2022

AbstractRecent studies have documented benefits of small, prescribed fire and wildfire for grassland‐dependent wildlife, such as lesser prairie‐chickens (Tympanuchus pallidicintus), but wildlife demographic response to the scale and intensity of megafire (wildfire >40,000 ha) in modern, fragmented grasslands remains unknown. Limited available grassland habitat makes it imperative to understand if increasing frequency of megafires could further reduce already declining lesser prairie‐chicken populations, or if historical evolutionary interactions with fire make lesser prairie‐chickens resilient. To evaluate lesser prairie‐chicken demographic response to megafires, we compared lek counts, nest density, and survival rates of adults, nests, and chicks before (2014–2016) and after (2018–2020) a 2017 megafire in the mixed‐grass prairie of Kansas, USA (Starbuck fire ~254,000 ha). There was a 67% decline in attending males on leks post‐fire and a 57% decline in occupied leks post‐fire. Despite population declines as indicated by lek counts, adult female breeding season survival () was similar pre‐ ( = 0.65 ± 0.08 [SE]) and post‐fire (0.61 ± 0.08), as was chick survival (pre‐fire: 0.23 ± 0.07; post‐fire: 0.27 ± 0.11). Nest survival appeared lower post‐fire (pre‐fire: 0.38 ± 0.06; post‐fire: 0.20 ± 0.06), but did not differ at the 95% confidence interval. Nest density of marked females declined 73% in areas burned by megafire. Although lesser prairie‐chickens persisted in the study area and we documented minimal effects on most demographic rates, reduced lesser prairie‐chicken abundance and reproductive output suggests full recovery may take >3 years. Increased propensity for megafire resulting from suppression of smaller fires, compounded by climate change and woody encroachment, may impose a short‐term (3–5 year) threat to already declining lesser prairie‐chicken populations.
Temple cooling increases parasympathetic activity and decreases pressure pain on the hand
Authors: Ye Di , Vo Lechi , Fairchild Timothy J. , Drummond Peter D. 
Published: December 2022

AbstractBackgroundApplying an ice cube to the temple (the conditioning stimulus) inhibits electrically evoked pain in the forearm. The present study aimed to determine whether temple cooling also inhibits pressure‐ and heat‐pain test stimuli in the upper limb and, if so, to investigate the intra‐session test–retest reliability of this response. Additional aims were to establish whether pain inhibition evoked by temple cooling was associated with parasympathetic activity; and to explore sex differences in response.MethodsThe sample consisted of 40 healthy adults (24 females). Heart rate was recorded continuously throughout the session. An ice cube (3 × 4 cm contact area) was applied for 1 min to the temple on the dominant side. Before and immediately afterwards, the pressure pain threshold was measured from the dorsal hand and sensitivity to heat (individually adjusted at baseline to elicit moderate pain) was measured from the ventral forearm. The procedures were repeated 15 min later.ResultsTemple cooling inhibited pressure pain on the hand but not heat pain on the forearm. However, test–retest reliability of pressure pain inhibition was poor. Heart rate decreased during temple cooling, consistent with a “diving” reflex. Males had stronger pressure pain inhibition, lower heart rate and higher overall autonomic activity than females. However, cardiac parasympathetic activation during temple cooling was comparable in both sexes and was unrelated to pain inhibition.ConclusionsThese findings indicate that temple cooling evokes pain inhibition that is stronger in males than in females. Cardiac parasympathetic activity does not appear to mediate this response.SignificanceThe conditioning stimulus in the conditioned pain modulation paradigm is often applied to the upper or lower limbs. This may confound pain‐inhibitory effects in people with peripheral neuropathy who typically have enhanced or diminished sensation in the extremities. Applying an ice cube at the temple area induces pain‐inhibitory effects on the upper limb after the ice is removed. Future research examining pain modulation in people with peripheral neuropathy may consider adopting temple cooling as the conditioning stimulus.
Determination of aflatoxin B1 by biomass derived porous carbon modified electrode with molecularly imprinted polymer
Authors: Chen Longyi , Mamat Xamxikamar , Aisa Haji Akber 
Published: December 2022

AbstractAflatoxin determination is imperative among agricultural and food products as the pollution of the toxic aflatoxin could cause severe hazards towards animals and human beings. Here, we prepared Elaeagnus gum derived porous carbon material as electrode modified material. In situ electropolymerization of molecularly imprinted polymer utilizing phenol as monomer and aflatoxin B1 as target template was further processed onto the porous carbon surface to achieve aflatoxin B1 sensing ability. This sensitive MIP sensor has a detection range from 5 pM to 100 pM of aflatoxin B1, an electrochemical sensitivity of 82.4 μA log(pM)−1 cm−2 with a detection limit of 1.7 pM, and a recovery rate of real sample measurement is 98.21 %. Good selectivity, fair repeatability and stability was confirmed. This work demonstrates the promising application of Elaeagnus gum derived porous carbon modified electrode sensor for food and drug monitoring.