bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Strategy of Precise Targeting and In Situ Oxygenating for Enhanced Triple-Negative Breast Cancer Chemophototherapy
Authors: Wu Manxiang , Chen Tianxiang , Wang Lianfu , Akakuru Ozioma Udochukwu , Ma Xuehua , Xu Jinshan , Hu Jiapeng , Chen Jia , Fang Qianlan , Wu Aiguo , Li Qiang 
Published: February 2022

Abstract
The absence of effective therapeutic targets and tumor hypoxia are the main causes of failure in the treatment of triple-negative breast cancer (TNBC). Biomimetic nanotechnology and tumor microenvironment (TME)-responsiveness bring hope and opportunity to address this problem. Here, we develop a core membrane nanoplatform (HM/D-I-BL) using hollow mesoporous manganese dioxide (HM) coated with biomimetic cancer cell membrane for enhanced chemotherapy/phototherapy via the strategy of precise drug delivery and hypoxia amelioration. Cancer cell membrane modification endows HM/D-I-BL with excellent homologous targeting and immune escape performance. The cellular uptake and fluorescence imaging studies confirmed that HM/D-I-BL can be accurately delivered to tumor sites. HM/D-I-BL also features efficient in situ O2 generation in tumor upon laser irradiation, and subsequently enhanced chemotherapy/phototherapy, pointing to its usefulness as a TME-responsive nanozyme to alleviate tumor hypoxia in the presence of H2O2. In addition, HM/D-I-BL showed good fluorescence and magnetic resonance imaging performances, which offers a reliable multimodal image-guided combination tumor therapy for precision theranostics in the future. In general, this intelligent biomimetic nanoplatform with its homotypic tumor targeting, in situ alleviation of tumor hypoxia and synergetic chemophototherapy would open up a new dimension for the precision treatment of TNBC.
An improved workflow for accurate and robust healthcare environmental surveillance using metagenomics
Authors: Shen Jiaxian , McFarland Alexander , Blaustein Ryan , Rose Laura , Perry-Dow K. , Hayden Mary , Young Vincent , Hartmann Erica 
Published: February 2022

Abstract
Effective surveillance of microbial communities in the healthcare environment is increasingly important in infection prevention. However, current workflows are insufficient for proper risk assessment. Upon evaluating and optimizing techniques, we recommend best practices and introduce a well-structured workflow for metagenomics-based environmental surveillance that is appropriate for low-biomass samples, distinguishes viability, and is quantitative. The workflow was developed using a representative microbiome sample, which was created by aggregating 120 surface swabs collected from a medical intensive care unit. We recommend liquid-liquid extraction, propidium monoazide treatment coupled with internal standards and absolute abundance profiling (e.g., using qPCR), and a machine learning-based model for sequencing depth calculation. In addition, whole-cell filtration and cultivation may be valuable under particular circumstances. This workflow will contribute to more accurate and robust environmental surveillance and infection prevention. Lessons gained from this study will also benefit the continuing development of methods in relevant fields.
Ensemble of Deep Learning Enabled Modulation Signal
Authors: N Senthil Kumaran V , G Indumathi , M Vijay 
Published: February 2022

Abstract
Automated classification of underwater acoustic signals allows to effectively utilize the acoustic spectrum by certain actions such as interference circumvention and marine mammal protections. The commonly utilized modulation techniques for underwater acoustic communication are MPSK (BPSK, QPSK, and 8PSK) and MQAM. Detection of modulation type of a received waveform enables sonar and communication in a similar bandwidth with least collision and it can determine the system functioning exterior to the permitted regime. Automated modulation classification models can make utilize of machine learning (ML) and deep learning (DL) techniques, particularly for underwater acoustic communication. With this motivation, this study designs a new ensemble of deep learning based modulation signal classification (EDL-MSC) models for underwater acoustic communication. Initially, an impulsive noise pre-processor was used for eliminating the impulse from the target signal. Besides, three deep learning models namely bi-directional long short term memory (BiLSTM), gated recurrent unit (GRU), and stacked sparse autoencoder (SSAE) models are used to derive features in the temporal waveform and square spectra of the pre-processed signal. In addition, black widow optimization (BWO) is applied for the optimal hyperparameter tuning of the DL models. Lastly, an ensemble of voting schemes is applied to integrate the outcome of the three DL models. The proposed model has the ability to perform effective modulation classification process in underwater acoustic communication. The performance validation of the EDLMSC technique takes place under several aspects and the comprehensive comparative analysis ensured the supremacy of the EDL-MSC technique over the recent approaches.
Litter Treatment Using Zeolite as a Management Protocol to Enhance Broiler Performance and Control Ammonia Emission in Broiler Production in the Humid Tropics of Nigeria
Authors: EZENWOSU CELESTINE chima , ANIZOBA NNENNA WILFRED , UDEH VALIENTINE VICTOR , NWOGA CAMELIOUS CHIJIOKE , ONODUGO MATTHEW ONYEKA , ONOCHIE CHARLSE OSITA , ONYIMONYI AMSYLEM EGO 
Published: February 2022

Abstract
A 9-week study was conducted to evaluate the effect of using zeolite as a litter management protocol to enhance broiler performance and control ammonia emission from litter. A total of 140 broiler birds (arbor acre) were used for the study. After brooding them together for 2 weeks as a way of stabilizing them, they were randomly assigned to 4 treatment groups labeled A-D in a completely randomized experimental design and each treatment was replicated 5 times with 7 birds per replicate. Treat2ment of the litter were as follows: treatment A litter (control) had no zeolite treatment, B (200g zeolite kg−3 litter), C (400g zeolite kg−3 litter), and D (600g zeolite kg−3litter).The experiment was divided into two phase. Starter phahse which lasted for 3weeks and finisher phase lasted for 4 weeks. Feed and water were provided ad libitum Results showed significant differences (P<0.05) in the birds’ values for total feed intake, average daily feed intake, total beyody weight, average daily weight gain, final body weight and feed conversion ratio in both phases in favor of treatment groups. The litter chemical analysis showed significant differences (P<0.05) in the bird’s mean values for mo5isture, uric acid, pH, phosphorous, ammonium nitrate, total volatile fatty acid, ammonium sulfate, ammonia, nitrogen, and ash among the study groups in both phases. Difference in litter microbial load values among the treatments were also significant (P<0.05). In conclusion, litter treatment using zeolite as a management protocol to enhance broiler performance and control ammonia emission was able to enhance performance of the birds by exerting diminutive effect on the precursors and microbial activities that are involve in ammonia production from the litter. It is therefore recommended that farmers can adopt this litter management protocol to reduce ammonia emissions in broiler houses without negatively affecting the growth of the birds.
Serial state switches between local and global computation
in ventromedial prefrontal cortex
Authors: Mehta Priyanka , Hayden Benjamin , Yoo Seng Bum 
Published: February 2022

Abstract
Adaptive behavior requires the ability to see both the forest and the trees - to focus on local events at some times, and to assess the global context at others. We hypothesized that these computational foci are associated with distinct neuronal states within one population. We recorded from neurons in area 14 of ventromedial prefrontal cortex (vmPFC) as macaques performed a foraging task that alternated between periods of within-patch searching and long (4-second) inter-trial intervals (ITIs) We found that neural activity during and between trials occupies semi-orthogonal subspaces, with greater dimensionality between trials. We found a double dissociation: between trials, patch richness is linearly decodable; whereas during trials, the prey value is linearly decodable. Finally, subjects calibrated their accept-reject behavior during the trial according to patch richness.We conclude that ostensible downtime prioritizes global processing and highlights how neural populations can accomplish foreground and background-oriented cognition by transforming the neural subspace.
Using Allogenous Structural Bone Graft for Uncontained Tibial Bone Defects ≥ 10 mm in Depth in Primary Total Knee Arthroplasty
Authors: Iwase Dai , Metoki Yukie , Kusumoto Yasuaki , Aikawa Jun , Fukushima Kensuke , Takano Shotaro , Mukai Manabu , Uchida Kentaro , Inoue Gen , Takaso Masashi 
Published: February 2022

Abstract
Background
In primary total knee arthroplasty (TKA), tibial bone defects ≥ 10 mm in depth often become uncontained defects, a condition most surgeons find challenging to treat. Although the allogenous bone graft is a useful method, complications such as infection and nonunion are likely to occur. There are several reports on the use of allogenous bone graft in revision TKA; however, few studies have investigated its use in primary TKA. We performed primary TKA using the allogenous bone graft as a structural bone graft to treat uncontained defects ≥ 10 mm in depth. This study aimed to assess the clinical and radiographical results after primary TKA with allogenous structural bone graft (ASBG).
Methods
Seventeen patients (mean age, 69.2 years) with a follow-up period of at least 7 years, were retrospectively reviewed. All cases had been treated for medial bone defects using the ipsilateral medial tibial allogenous bone. Clinical evaluation included the assessment of the knee and function scores and knee angle, and the hip-knee-ankle (HKA) angle, bone union, and radiolucent line (RL) were assessed radiologically.
Results
The mean depth of the medial tibial defects after tibia cutting was 16.8 mm. Nonunion occurred in one case, and RL occurred in another. We observed a significant difference when the preoperative knee score and HKA angle of patients was compared with that at 1 year postoperatively and the final evaluation. No major complications were observed.
Conclusion
The ASBG technique produced favorable surgical outcomes and may be an acceptable procedure for managing uncontained tibial bone defects ≥ 10 mm in depth in primary TKA.
Prediction of Major Adverse Cardiovascular Events (MACCE) Following Percutaneous Coronary Intervention Using ANFIS-PSO Model
Authors: Karsidani Sahar Dehdar , Farhadian Maryam , Mahjub Hossein , Mozayanimonfared Azadeh 
Published: February 2022

Abstract
Background: This study aimed to use the hybrid method based on an adaptive neuro-fuzzy inference system (ANFIS) and particle swarm optimization (PSO) to predict the occurrence of major adverse cardiac and cerebrovascular events (MACCE) of patients underwent angioplasty.Method: This is a retrospective cohort study comprised a total of 220 patients (69 women and 151 men) who underwent coronary angioplasty in Ekbatan medical center in Hamadan city, Iran between March 2009 until March 2012. The occurrence and non-occurrence of MACCE, (including death, CABG, stroke, repeat revascularization) were considered as a binary outcome. The performance of ANFIS models for predicting MACCE was compared with ANFIS-PSO and logistic regression.Results: Ninety-six patients (43.6%) experienced the MACCE event after ten years of follow-up. In multivariate analysis based on logistic regression model, variables such as age (OR = 1.05), smoking (OR = 3.53), diabetes (OR = 2.17) and stent length (OR = 3.12) had a significant effect on MACCE occurrence. Comparing the prediction performance of the models showed that the ANFIS-PSO model had higher accuracy (89%) compared to the ANFIS (81%) and logistic regression (72%) in the prediction of MACCE.Conclusion: The performance of ANFIS-PSO has a minimum error and maximum accuracy compared to other models in the prediction of MACCE. Application of this model is recommended for intelligent monitoring of these patients, the classification of high-risk patients and the allocation of necessary medical and health resources based on the needs of these patients.
Implementation evaluation of a leadership strengthening intervention for improved family experience in a private paediatric care hospital, Pakistan
Authors: Rasheed Muneera A. , Hussain Ayesha , Hashwani Amin , Kedzierski Johannes , Hasan Babar S. 
Published: February 2022

Abstract
Background: A study from a tertiary care center in Pakistan demonstrated that a leadership strengthening intervention led to improved family experience of care outcomes. The objective of the current paper is to assess the implementation of this intervention and identify barriers and facilitators to inform sustainability and scalability. Methods: A working group designed the intervention using a theory-of-change model to strengthen leadership development to achieve greater employee engagement. The interventions included: i) purpose and vision through purpose-driven leadership skills trainings; ii) engaging managers via on-the-job mentorship programme for managers, iii) employee voice i.e., facilitation of upward communication to hear the employees using Facebook group and subsequently inviting them to lead quality improvement (QI) projects; and iv) demonstrating integrity by streamlining actions taken based on routine patient experience data. Implementation outcomes included acceptability, adoption, fidelity across degree & quality of execution and facilitators & barriers to the implementation. Data analyzed included project documentation records and posts on the Facebook group. Analysis indicated acceptability and adoption of the intervention by the employees as178 applications for different QI projects were received. Leadership sessions were delivered to 455 (75%) of the employees and social media communication was effective to engage employees. However, mentorship package was not rolled out nor the streamlined processes for action on patient experience data achieved the desired fidelity. Only 6 QI projects were sustained for at least a year out of the 18 approved by the working group. Facilitators included leadership involvement, real-time recognition and feedback and value-creation through participation by national and international celebrities. Challenges identified were the short length of the intervention and incentives not being institutionalized. The authors conclude that leadership strengthening through short training sessions and on-going communications facilitated by social media were the key processes that helped achieve the outcomes. However, a long-term strategy is needed for individual managerial behaviours to sustain.
Ludangshen Oral Liquid for Treatment of Convalescent COVID-19 Patients: A Randomized, Double-Blind, Placebo-Controlled Multicenter Trial
Authors: an Xuedong , peng bo , huang xiaodong , jiang hongmei , xiong zhang e , zhang hong , lian fengmei , ba yuanming , tong xiaolin 
Published: February 2022

Abstract
Objective To explore the effect of Ludangshen Oral Liquid for treatment of convalescent patients with coronavirus disease 2019 (COVID-19) with randomized, double-blind, placebo-controlled multicenter method.Methods200 convalescent COVID-19 patients who had symptoms related to decreased digestive and respiratory function were randomly divided to either receive Ludangshen Oral Liquid or placebo for 2 weeks. The severity of clinical symptoms including fatigue, anorexia, abdominal distension, loose stools, and shortness of breath were assessed by visual analogue scale and observed at before and after treatment. The improvement and resolution rates of clinical symptoms were evaluated. Full analysis set (FAS) and per-protocol set (PPS) were used for statistical analyses. Adverse events were recorded during the study. Results8 patients did not complete the study. After 2 weeks of treatment, both FAS and PPS results showed that patients in Ludangshen group had significantly lower score of fatigue, anorexia, loose stools, and shortness of breath than placebo group (P < 0.05), while there was no significant difference in distention (P > 0.05). The improvement rate of fatigue, anorexia, distension, loose stools and shortness of breath were significantly higher in Ludangshen group (P < 0.05), as well as the resolution rates (P < 0.05) except for shortness of breath (P > 0.05). There were two cases of adverse events, with one nose bleeding in Ludangshen group and one headache in placebo group. ConclusionThe study suggested that two weeks of Ludangshen Oral Liquid treatment may have certain effects for convalescent COVID-19 patients on improving digestive and respiratory symptoms including fatigue, anorexia, loose stools and shortness of breath, which may be one of the choices for management of convalescent COVID-19 patients with digestive and respiratory symptoms.
The association between dietary antioxidant indices and cardiac disease: Baseline data of Kharameh cohort
Authors: keshani parisa , jalali Maryam , ghoddusi masomeh , rezaeianzadeh ramin , Hosseini Seyed Vahid , rezaeianzadeh Abbas 
Published: February 2022

Abstract
Background:Oxidative stress contributes to the development of cardiovascular disease. Tools for evaluating the anti-inflammatory and antioxidative characteristics of an individual’s diet as a whole may be valuable for assessing the combined effects of dietary antioxidants on health. This population-based study aimed to investigate the association between dietary antioxidants and cardiac disease.Methods:In this population-based cross-sectional study, 10439 individuals aged 40-70 years were recruited during 2014-2017 in Kherameh cohort study which is a part of the Prospective Epidemiological Research Studies in Iran (PERSIAN). The food frequency questionnaire (FFQ) with 130 food items was used to assess the dietary intakes. Vitamin A, E, C, selenium, zinc and Manganese intakes were used to compute dietary antioxidant index (DAI) and dietary antioxidant quality score (DAQs).Results:The participants’ mean age was 52.1± 8.3 years. Among all, 4356 (41.7%) were overweight and 1892 (18.1%) were obese. According to the results, odds of cardiac diseases decreased by increasing DAI score (OR=0.80, Pvalue <0.001). , Odds of cardiac diseases increased by lower DAQS after adjusting for demographic variables including age, sex, BMI, Marital status and hypertension (OR=0.799, P value=0.002)Conclusion:The role of anti-oxidants in reducing the odds of cardiovascular disease is very important. Our results highlighted that DAQS and DAI had protective effect on the odds of cardiovascular disease. Therefore, it is suggested that anti-oxidants as zinc, manganese, selenium, and vitamins A, E and C should be taken through food to reduce the risk of the disease.
Low-dose Polyethylenimine-Mediated Transfection to Generate Human Induced Pluripotent Stem Cell: an optimized protocol
Authors: Shayestehfar Monir , Farahi Sara , Azar Behjat Kheiri Yeganeh , Memari Amirhossein , Baluchnejadmojarad Tourandokht , Faghihi Faeze 
Published: February 2022

Abstract
Human dermal fibroblasts (HDF) can be reprogrammed through different strategies to generate human induced pluripotent stem cells (hiPSCs). However, most of these strategies require high-cost materials and specific equipment not readily accessible in most laboratories. Hence, liposomal and virus-based techniques can replace with polyethyleneimine (PEI)-mediated transfection to overcome these challenges. However, few researchers have addressed the PEI's ability to transfect HDFs. This study used PEI reagent to transfer oriP/EBNA1-based vector into HDFs to produce hiPSC lines. We first described conditions allowing the efficient transfection of HDFs with low cytotoxicity and without specific types of equipment and optimized several parameters relevant to the transfection procedure. We then monitored the effect of different N/P ratios on transfection efficiency and cytotoxicity using flow cytometry and fluorescent microscopy. By the results, we found that transfection efficiency was greatly affected by plasmid DNA (pDNA) concentration, PEI concentration, order of combining reagents, serum presence in polyplexes, and the duration of serum starvations. Moreover, using the optimized condition, we found that the N/P ratio of 3 achieved the highest percentage of HDFs positive for pGFP (~ 40%) with minimal cell toxicity. We finally generated hiPSCs using the optimized protocol and oriP/EBNA1-based vectors. We confirmed hiPSC formation by characterizing tests: Alkaline Phosphatase (AP) staining, immunocytochemistry (ICC) assay, real-time PCR analysis, in vitro differentiation into three germ layers, and karyotyping test. In conclusion, our results indicated that 25kD branched PEI could efficiently transfect HDFs toward generating hiPSCs via a simple, cost-effective, and optimized condition.
Comparing the 1-year clinical outcomes of polymer-based paclitaxel-eluting stent implantation between hemodialysis and non-hemodialysis patients: A prospective, observational, single-center study”.
Authors: Nakama Tatsuya , Torii Sho , Kojima Shunsuke , Muraishi Makio , Arai Junya , Azumi Yuta , Asano Kazuhiro , Takafuji Hiroya , Obunai Kotaro , Watanabe Hiroyuki 
Published: February 2022

Abstract
Background: Previous studies have shown favorable clinical outcomes of polymer-based paclitaxel-eluting stents (PB-PESs) in patients with femoropopliteal artery (FPA) disease. However, the effectiveness of PB-PES in hemodialysis (HD) patients has not been reported. The aim of this study was to compare the clinical performance of PB-PESs between HD and non-HD patients with symptomatic FPA disease.Materials and Methods: Between January 2019 and February 2020, 52 consecutive patients (mean age: 75.5 years; 42 men) underwent PB-PES implantation for symptomatic FPA diseases. Of these, 29 patients received HD and were classified into the HD cohort. The remaining 23 patients were classified into the non-HD cohort. Clinical outcomes were compared between the two cohorts. The primary efficacy endpoint was the primary patency at 12 months, and the primary safety endpoint was the 12-month incidence of major adverse cardiovascular and limb events (MACLEs), which were defined as the composite of the incidence of all-cause death, major amputation, stent thrombosis, and clinically driven target lesion revascularization (CD-TLR).Results: The overall primary patency rate was 93.3%. No significant difference existed in primary patency between the HD and non-HD cohorts (91.7% vs. 95.0%, P=0.577). The overall incidence rate of MACLEs was 16.6%, and the incidence rate was not significantly different between the HD and non-HD cohorts (20.2% vs. 13.3%, P=0.954). The incidence of stent thrombosis was not different between the HD and non-HD cohorts (8.3% vs. 5.0%, P=0.956).Conclusion: PB-PESs have exceptional 1-year primary patency and acceptable safety in HD patients and non-HD patients.Level of Evidence: Level 3, nonrandomized, follow-up study