bluuebunny/crossref_metadata_2025_split huggingface dataset:
How are left-sided rib fractures associated with splenectomy in cases of splenic injury? A multicentre cohort study from urban India.
Authors: Banker Amay , Wärnberg Martin Gerdin , Gadgil Anita , Sarang Bhakti , Prajapati Ramlal , Soni Kapildev , Khajanchi Monty 
Published: May 2022

Abstract
Background
Any fracture to the rib cage, particularly the left sided ribs, implies a high impact trauma and higher predisposition to splenic injury. Our hypothesis is that injuries causing fractures to the left sided ribs may indicate a high-grade splenic injury which may require splenectomy. This would help the surgeon working in rural and limited resource settings, to plan splenectomy in hemodynamically stable patients with an undetermined grade of splenic injury. With this hypothesis in mind, we aimed to determine how fractures to the left sided ribs, in cases of splenic injury, are associated with splenectomy.
Methods
We performed a subgroup analysis of patients with splenic injury from a prospective trauma registry study named ‘Towards Improved Trauma Care Outcomes’ in India. Categorical variables were analyzed using the chi square test and a binary logistic regression was developed to assess the significance of continuous variables.
Results
During the study period, a total of 16047 patients were included. Of these, 267 patients suffered from splenic injury and 70 patients required splenectomy. Fractures of the left sided ribs was not associated with splenectomy. Injury severity score (ISS), a lower systolic blood pressure and oxygen saturation at arrival, requirement of blood transfusions within 24 hours of admission and a higher grade of splenic injury (grade 4 and grade 5) were associated with splenectomy.
Conclusion
In contradiction to our initial hypothesis, we found that left sided rib fractures were not significantly associated with splenectomy. Grade of splenic injury was an important determinant of splenectomy.
CRISPR-Cas9 genome engineering as a novel prognostic tools for Prostate cancer: long non-coding RNAs the predictive diagnostic index of targeted liposomal-coated compounds in prostate cancer therapy
Authors: Kadry mai o. , Abdel-Megeed Rehab M. 
Published: May 2022

Abstract
Background: CRISPR/Cas9 is a recently explored genomic editing system that has greatly altered the technique that scientists study genes and their functions in mammalian systems. Cas9 is regulated via guide RNAs that match the targeted DNA in cleavage to modify the respective gene. The incessant advancement in prostate cancer (PC) modeling has directed not only to innovate resources of recognizing the signaling pathways overriding prostate cell carcinoma, but it has also created a vast reservoir for complementary tools to examine therapies counteracting this type of cancer. Various cultured somatic rat models for prostate cancer have been produced that nearly resemble human prostate cancer. Nano-medicine can passively target cancer cells via the enhanced permeability and retention phenomenon or by conjugation with specific ligand, contributing to reduced systemic side-effects and increased efficacy. This article highlights the utilization of PEG-targeted liposomal loaded nano-medicine for potential treatment of prostate cancer and clarifies the CRISPR/Cas9 variation accompanied with prostate cancer.Methods and Results: PC is induced experimentally in western rat model via a diet containing 0.75 ppm of ethinyl estradiol for 3 weeks and a single SC. injection of 50 mg/kg body weight of 3,2'- dimethyl-4-aminobiphenyl estradiol (3,2'-DAE) followed by treatment via PEG-2000 targeted liposomal-coated compounds such as PEG-liposomal dexamethasone, PEG-liposomal doxorubicin and PEG-liposomal curcumin (3 mg/kg IP) for four weeks in a comparative study to their non-targeted analogue dexamethasone, doxorubicin and curcumin. 3, 2'- dimethyl-4-aminobiphenyl estradiol elicit prostate cancer in western rats within 5 months. Simultaneous supplementations with these liposomal compounds influence on prostate cancer; tumor markers were investigated via prostate-specific antigen (PSA) and CRISPR/Cas9 gene editing. Several long non-coding RNAs have been indicated to be deregulated in prostate cell carcinoma, including MALAT1. On the other hand, gene expression of apoptotic biomarkers focal adhesion kinase (AKT-1), phosphotidyl inistol kinase (PI3K) and glycogen synthase kinase 3 (GSK-3) was also investigated and further confirming these results via histopathological examination. Conclusion: PEG-liposomal loaded dexamethasone, doxorubicin and curcumin can be considered as promising therapeutic agents for prostate cancer via modulating CRISPR/Cas9 gene editing and long non coding gene MALAT1.
Long non-coding RNA PTTG3P promotes hepatocellular carcinoma growth via modulating nuclear-cytoplasm shuttle of ILF2 and stabilizing TOP2A mRNA
Authors: bai hansong , zhang hangyue , Lyu jiahua , Liang Long , Jia Hongyuan , Zeng Ming , Li Tao 
Published: May 2022

Abstract
Background: PTTG3P has been characterized as an oncogenic lncRNA in hepatocellular carcinoma (HCC). However, the underlying mechanisms have not been fully understood. This study aimed to explore the functional partner of PTTG3P in modulating HCC malignant phenotypes. Methods: Hep3B and HepG2 were used as representative HCC models. Cellular and molecular studies were performed using RNA pull-down/mass spectrometry (MS), RNA immunoprecipitation (RIP)-qPCR, Chromatin immunoprecipitation (ChIP)-qPCR, and immunofluorescent (IF) staining. Results: PTTG3P interacts with both ILF2 and ILF3. The binding between PTTG3P and ILF2 helps maintain the cytoplasmic distribution of ILF2. Together with ILF2, PTTG3P binds to the 3'UTR of TOP2A. PTTG3P depletion impairs the binding and reduces TOP2A mRNA stability. Meanwhile, PTTG3P depletion is associated with nuclear accumulation of ILF2 and increased binding of ILF2 to the promoter region of TOP2A. Dual-luciferase assay confirmed that ILF2 activates the TOP2A promoter and increases its transcription. Conclusion: this study revealed that when PTTG3P is overexpressed in HCC tumor cells, it mainly acts as a TOP2A mRNA stabilizer, via recruiting the ILF2/ILF3 complex to the 3’UTR of TOP2A mRNA. However, PTTG3P depletion results in nuclear accumulation of ILF2 and subsequent transcriptional activation of TOP2A. This might be utilized by HCC cells to compensate the loss of PTTG3P and associated TOP2A instability. These findings help expand our understanding of the oncogenic mechanisms of PTTG3P in HCC.
Therapeutic effect of silibinin on vanadium-induced chronic lung injury in mice
Authors: Im Hobin , Kim Eungyung , Kwon Hong Ju , Kim Hyeonjin , Cho Na Eun , Ko Jiwon , Sung Yonghun , Jang Soyeon , Kim Sung-Hyun , Lee Eun Jung , Yoon Duhak , Kwon Woo-Sung , Ryoo Zae Young , Yi Jun Koo , Kim Myoung Ok 
Published: May 2022

Abstract
Background: Silibinin has shown therapeutic effects in various inflammatory and cancer models. However, the therapeutic effect and underlying mechanism in V2O5-induced pulmonary inflammation, which consists of particulate matter and metal components, have not been identified. We examined the potential anti-inflammatory activity and underlying mechanism of silibinin in a vanadium-induced lung injury model. Results: Silibinin treatment resulted in a significant restoration of cell viability and reduced infiltration of inflammatory cells in a histological analysis. In addition, silibinin significantly reduced the expression of the pro-inflammatory cytokines, TNF-α, IL-6, and IL-1ꞵ. Furthermore, silibinin reduced inflammation by down-regulating the MAPK and NF-kB signaling pathways and the NLRP3 inflammasome. Treatment with silibinin significantly reduced the NLRP3 expression levels in lung tissue. Conclusion: These results indicate that silibinin exhibits anti-inflammatory effects in V2O5-induced inflammatory mice.
Does Preventive Single Dose of Intravenous Dexketoprofen Reduce Pain and Swelling After Orthognathic Surgery? A Prospective, Randomized, Double Blind Clinical Trial
Authors: Kaba Yusuf Nuri , Demirbaş Ahmet Emin , Kütük Nükhet , Canpolat Dilek Günay , Alkan Alper 
Published: May 2022

Abstract
Objectives
The purpose of this study was to evaluate the effect of a single-dose intravenous dexketoprofen administration for preventive analgesia on postoperative pain and reducing swelling in double jaw surgery.
Material and Methods
The authors designed a prospective, randomized, and double-blind cohort study. Patients who have Class III malocclusion were randomly divided in two groups. 50 mg intravenous dexketoprofen trometamol were administrated 30 minutes before incision in treatment group, while intravenous sterile saline was administrated 30 minutes before incision in placebo group. The primary predictor variable was treatment group. Primary outcomes were pain, swelling and 24-hour opioid intake. Patient- controlled analgesia with tramadol was given for management of postoperative pain. Other variables were demographic and operation related parameters. Visual analogue scale was used to evaluate postoperative pain. Stereophotogrammetry system was used to measure postoperative swelling. Data were analysed using two independent samples t test and Mann Whitney U test.
Results
The study sample was composed of 30 patients with a mean age of 20,63 years and 21 were female. Preemptive dexketoprofen administration decreased postoperative tramadol consumption by 25.9% compared to placebo group, and there was a statistically significant decrease in VAS scores (p < 0,05). There was no statistically significant difference between the groups in terms of swelling (p > 0,05).
Conclusions
Preventive administration of intravenous dexketoprofen provides adequate analgesic effect in the postoperative 24-hour period and reduces opioid consumption in orthognathic surgery.
Mental and psychosocial health among youth after their first psychiatric hospitalization: a feasibility study
Authors: Ferro Mark A , Chan Christy KY , Vanderkooy John D , Horricks Laurie , Duncan Laura , Lipman Ellen L 
Published: May 2022

Abstract
Objective: This pilot study investigated the feasibility of studying 12-month readmission of youth aged 10-16 years following their first psychiatric hospitalization and changes in youth mental and psychosocial health prospectively. Results: Inpatient youth with a first psychiatric hospitalization and their parents were recruited from a regional hospital in Canada. Data were collected at recruitment, and at 3-, 6-, and 12-months post-discharge. Repeated measures ANOVA was performed to assess changes in health outcomes. Nineteen eligible youth were approached and 15 (78.9%) consented to participate (13.9±2.0 years, 73.3% female). Eleven youth (73.3%) gave permission to contact their parents, all of whom participated (39.2±7.6 years). Four youth dropped out of the study (26.7%) and six youth-parent dyads completed all four follow-ups. The readmission rate was 20.0% (n=3) over 12 months. Significant changes in youth-reported symptoms of conduct disorder (F=3.0, p=0.06) and adverse childhood experiences (F=3.4, p=0.05) were found. Changes in parent-reported youth mental health symptoms (F=3.1, p=0.06), particularly among internalizing disorders, youth health-related quality of life (F=11.3, p<0.01), and youth disability (F=2.7, p=0.08) were significant. This preliminary work demonstrates the feasibility of, and need to, engage youth and their families to understand their mental and psychosocial health during this vulnerable time in their lives.
Hydrothermal mineralization and mineral chemistry of arsenides and sulfarsenides in the Fe-Co-Ni-As-S system and introduction of three unknown minerals from the Port Radium IOCG deposit, Northwest Territories, Canada
Authors: Somarin Alireza , Zhou Li 
Published: May 2022

Abstract
The Port Radium deposit is the result of intrusion of the Mystery Island felsic-intermediate plutonic complex into the ~ 1.885 Ga supracrustal marine sedimentary rocks and volcanic suits, which formed a variety of mineralization styles from iron oxide-copper-gold (IOCG) to epithermal. Extensive and intensive hydrothermal alterations including albitic, magnetite-actinolite-apatite, potassic ± albitic, phyllic, and propylitic prepared a ground for the main sulfide, sulfarsenide and uraninite mineralization during last stage of hydrothermal activity. Scarce and weak sulfide mineralization started from the beginning of the hydrothermal activity and reached to the strongest state during epithermal stage. In addition to the common arsenides and sulfarsenides, three unknown sulfarsenides were also found: (Co0.96Ni0.45Fe0.03)S0.27As4 which is probably a solid solution product of cobaltite and gersdorfite; (Ni2.13Co0.38)S0.97As4 and (Ni1.83Co0.83)S1.25As4 which are chemically comparable to the Port Radium rammelsbergite with substantial addition of S and Co; they could also be the solid solution product of gersdorfite-cobaltite or safflorite-rammelsbergite.
Steps dominate gas evasion from a mountain headwater stream
Authors: Botter Gianluca , Carozzani Anna , Peruzzo Paolo , Durighetto Nicola 
Published: May 2022

Abstract
Step-and-pools are dominant morphologic traits of high-energy streams, where climatically- and biogeochemically-relevant gases are processed, transported to downstream ecosystems or released into the atmosphere. Yet, capturing the signature of the small-scale morphological complexity of channel forms on large-scale river outgassing represents a fundamental unresolved challenge. Here, we combine theoretical and experimental approaches to assess the contribution of localized steps to the gas evasion from river networks. The framework was applied to a representative, 1 km-long mountain reach in Italy, where carbon dioxide concentration drops across several steps and a reference segment without steps were measured under different hydrologic conditions. Our results indicate that local steps lead the reach-scale outgassing, especially for high and low discharges.
These findings suggest that step-pools are key missing components of existing scaling laws used for the assessment of gas fluxes across water-air interfaces. Therefore, global evasion from rivers may differ substantially from previously reported estimates.
Hydrogel-extraction technique for non-invasive detection of blue fluorescent substances in plant leaves
Authors: Iwasa Shigeyuki , Kobara Yuso , Maeda Katsumi , Nagamine Kuniaki 
Published: May 2022

Abstract
This paper reports a new hydrogel extraction technique for detecting blue fluorescent substances in plant leaves. These blue fluorescent substances were extracted by placing a hydrogel film on the leaf of a cherry tomato plant infected with Ralstonia solanacearum; herein, chlorogenic acid was confirmed to be a blue fluorescent substance. The wavelength at the maximum fluorescence intensity of the film after the hydrogel extraction was similar to that of the methanolic extract obtained from the infected cherry tomato leaves. Chlorophyll was not extracted from the hydrogel film because no fluorescence peak was observed at 680 nm. Accordingly, the blue fluorescence of the substances extracted from the hydrogel film was not quenched by the strong absorption of chlorophyll in the blue light region. This hydrogel extraction technique can potentially detect small amounts of blue fluorescent substances and the changes in its amount within the leaves of infected plants. These changes in the amount of blue fluorescent substances in the early stages of infection can be used to detect presymptomatic infections. Therefore, hydrogel extraction is a promising technique for the noninvasive detection of infections before onset.
Identification of two potential biomarkers of Graves' Disease by bioinformatics analyses based on Gene Expression Omnibus datasets with immune cells infiltration.
Authors: Zhang Yihan , Wei Jia , Zhou Hong , Li Bingxin , Chen Ying , Qian Feng , Liu Jingting , Xie Xin , Xu Huanbai 
Published: May 2022

Abstract
Background: Graves' disease (GD) is an autoimmune disease, the incidence of which is increasing year by year. And it still needs long life therapy. So, the detailed pathogenesis of GD still needs further study. Method: In our study, differentially expressed genes (DEGs) were derived from online Gene Expression Omnibus (GEO) microarray expression dataset GSE71956. Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes (KEGG) analysis was used to enrichment analysis. Protein-protein interaction (PPI) network analyses were used to identify the hub genes. Then hub gene was validated by qPCR. Next CIBERSORT analysis was used to further explore the immune infiltration among hub genes. ROC curve was used to analysis the specificity and sensitivity of hub genes. Result: 44 DEGs were screened out from GEO dataset. GO and KEGG analysis of DEGs shows that GD involves immunity and metabolism. Two hub genes EEF1A1, EIF4B were recruited from PPI network, and was validated by qPCR ( p < 0.05). Immune cell infiltration analysis revealed that plasma cells, T cells CD4 memory resting, T cells follicular helper, dendritic cells activated, mast cells activated were correlated with GD. We are the first to demonstrate the expression of EEF1A1, EIF4B has significant positive correlations with infiltrating levels of T cells CD4 memory resting. The AUC of EEF1A1 ROC analysis is 0.687 and ROC of EIF4B is 0.733.Conclusion : Our study revels two hub genes, EEF1A1, EIF4B, which were associated with resting memory CD4 + T cells , may be the potential molecular biomarkers and therapeutic targets of GD.
Analysis and Risk Assessment of Pharmaceutical Residues in Fish from Three (3) Water Bodies in Ghana.
Authors: Duncan Albert . Ebo , Adoko Christian , Osei-Marfo Martha , Barnie Samuel , Sakyi Aboagye George , Adjei Joseph 
Published: May 2022

Abstract
Pharmaceutical pollution of rivers in Ghana is overlooked due to the overshadowing problem of illegal mining. However, a greater percentage of water bodies (especially rivers) in Ghana are potentially exposed to pharmaceutical waste and pollution. The uncontrolled disposal of pharmaceuticals from industry, hospitals, households, municipalities, and other consumers into rivers is very alarming. Humans continue to fish from these polluted water bodies with little concern or knowledge of the potential threat the pharmaceutical residues accumulated in these fishes pose to their health. In the present study, the residues of one (1) antibiotic (Chloramphenicol), five (5) hormones (progesterone, 17-b-estradiol, estrone, 17-a-ethynylestradiol, and testosterone), three (3) environmental contaminants (4-para-nonylphenol, 4-tert-octylphenol, and Bisphenol A), one (1) Barbiturate (Primidone) and one (1) analgesic (Diclofenac sodium salt), were investigated from fish samples from the rivers Pra, Narkwa, and the Volta. The fish samples were analyzed using high-performance liquid chromatography (HPLC). The results show a high concentration of drugs in River Pra in comparison to those in River Narkwa and Volta. The hazard quotients for the environmental contaminants were all above one (1), except Bisphenol-A. Furthermore, hazard quotients from this study suggest that consumers of fish from any of the three rivers stand a hazard risk of Chloramphenicol (19), 17-a-ethynylestradiol (4), Estrone (1.366), Diclofenac sodium salt (3.29), Progesterone (4.598), 4-tert-octylphenol (87.2) and 4-para-nonylphenol (7.252), but negligible risk against E2 (0.687), Primidone (0.014), Testosterone (0.16), and Bisphenol A (0.642). Of the fish species studied the highest concentration of all pharmaceuticals put together is found in Clarias gariepinus, then Labeo senegalensis, and Chrysichthys nigrodigitatus in that order.
Edible film production using Aronia melanocarpa for smart food packaging
Authors: Ozcan Arif , Kandirmaz Emine Arman 
Published: May 2022

Abstract
While smart packaging systems protect the food, they also provide information about the shelf life of the content. This information is provided by the formation of severe shifts in color as a result of the reaction of the gases released by the decomposition of the food, the colorant used in the smart packaging. Inks or films used in smart packaging systems may have this feature. Since both ink and film material come into direct contact with the food product in smart packaging, it is more suitable for the intended use of the packaging to be produced from edible and natural resources. In this study, it is aimed to investigate the production of films and their use in active packaging systems by using natural source starch and another bio source black chokeberry (Aronia melanocarpa) fruit in order to produce smart packaging. In this context, in this study, firstly, the coloring matter was extracted from black chokeberry (Aronia melanocarpa) fruit with ethyl alcohol. The monomeric anthocyanin and total phenol content of extracted Aronia melanocarpa were determined by pH differential method. Colorimetric edible films sensitive to pH change were produced by using starch biopolymer by adding different amounts of the obtained Aronia extract. The chemical structure of the produced films was determined by ATR-FTIR, color properties were determined by Spectrophotometer, contact angles were determined by Goniometer. In addition, swelling ability was calculated depending on pH. Disk diffusion method was applied to the obtained films against gram-positive and gram-negative bacteria, and the antibacterial properties of the films were determined. As a result; The swelling ability of the films prepared with Aronia and starch decreased compared to the blank film. The prepared films maintained their stability in all pH scales, including high acid and base. While the edible films produced have a pink-red color at acidic pHs, the color turns to dark blue at basic pHs. This shows that the color of the films will change depending on the nitrogen output from the spoiled meat and can be used as smart packaging. In addition, the fact that the films produced have inhibitory properties against gram-positive bacteria also add an extra feature to the packaging material, as it will protect the food against degradation.
The protective effect of gingival mesenchymal stem cells on radiation-induced skin damage and its mechanism
Authors: Nie Shasha , Ren Chunhua , Cai Hui , Sun Hao , Liu Fengting , Du Liqing , Wang Qin , Xu Chang , Liu Yang , He Ningning , Ji Kaihua , Wang Jinhan , Zhang Manman , Song Huijuan , Gu Yeqing , Sun Xiaohui , Wang Yan , Liu Qiang 
Published: May 2022

Abstract
Abstract
Nuclear pollution is one of the main environment pollution. And Almost all of the waste of the nuclear accident will cause human skin radiation damage because of the special physiological location and the characterics of the skin cells. Different from other skin injuries, cutaneous radiation injury (CRI) is caused by the loss of a large number of stem cells which is very sensitive to radiation. Gingiva mesenchymal stem cells (GMSCs) have the characteristics of self-renewal, pluripotent differentiation, and immune regulatory cells both in vitro and in vivo and are easy to obtain without carcinogenicity than other stem cells. To prolong the residence time of the stem cells at the site of injury, Nap-GDFDFpDY (pY-Gel) self-assembling peptide hydrogels have been used in this study. Results revealed GMSCs can obviously alleviate skin injury induced from high-dose IR in mice and promote skin healing. And in cellular experiments, GMSCs conditioned medium (CM) can promote the proliferation and migration of skin cells, enhance the ability of DNA damage repair and reduce the inflammatory response. In addition, The findings of this study suggest that the mechanism may involve the activation of the EGFR/STAT3 signaling pathway in skin cells to promote the repair of skin cells after exposure to radiation.