bluuebunny/crossref_metadata_2025_split huggingface dataset:
Early Prosperity of Iron Bacteria at the End of the Paleoproterozoic Era
Authors: Qiu Yifan , Qin Liping , Huang Fang , Zhao Taiping , Li Yiliang 
Published: April 2022

AbstractVarious iron formation (IFs) document the interplay between the geosphere and the early biosphere during their co‐evolution. At the transition between the Paleo‐ and Mesoproterozoic eras, the oxidation of the atmosphere and ocean resulted in the waning of the typical marine deposited banded IFs. Here, we report the ∼1.7 Ga Yunmengshan IF at the southern margin of the North China Craton deposited in the intertidal to upper subtidal zone belonging to river delta of coastal environments. The combined REE + Y patterns and negative δ56Fe with positive δ53Cr values reveal terrestrial precipitation of iron oxide and an enhanced continental oxidative weathering at the end of Paleoproterozoic era due to increased atmospheric oxygen level. The oxygen level happened to have facilitated the early prosperity of microaerophilic Fe(II)‐oxidation bacteria on Earth's terrestrial surface, which is recorded by the coastal shallow water iron deposits globally characterized by their small scale and subaerial features.
A new horizon of precision medicine: combination of the microbiome and extracellular vesicles
Authors: Yang Jinho , Shin Tae-Seop , Kim Jong Seong , Jee Young-Koo , Kim Yoon-Keun 
Published: April 2022

AbstractOver several decades, the disease pattern of intractable disease has changed from acute infection to chronic disease accompanied by immune and metabolic dysfunction. In addition, scientific evidence has shown that humans are holobionts; of the DNA in humans, 1% is derived from the human genome, and 99% is derived from microbial genomes (the microbiome). Extracellular vesicles (EVs) are lipid bilayer-delimited nanoparticles and key messengers in cell-to-cell communication. Many publications indicate that microbial EVs are both positively and negatively involved in the pathogenesis of various intractable diseases, including inflammatory diseases, metabolic disorders, and cancers. Microbial EVs in feces, blood, and urine show significant differences in their profiles between patients with a particular disease and healthy subjects, demonstrating the potential of microbial EVs as biomarkers for disease diagnosis, especially for assessing disease risk. Furthermore, microbial EV therapy offers a variety of advantages over live biotherapeutics and human cell EV (or exosome) therapy for the treatment of intractable diseases. In summary, microbial EVs are a new tool in medicine, and microbial EV technology might provide us with innovative diagnostic and therapeutic solutions in precision medicine.
Sphingosine-1-phosphate hinders the osteogenic differentiation of dental pulp stem cells in association with AKT signaling pathways
Authors: Choi Bongkun , Kim Ji-Eun , Park Si-On , Kim Eun-Young , Oh Soyoon , Choi Hyuksu , Yoon Dohee , Min Hyo-Jin , Kim Hyung-Ryong , Chang Eun-Ju 
Published: April 2022

AbstractSphingosine-1-phosphate (S1P) is an important lipid mediator that regulates a diverse range of intracellular cell signaling pathways that are relevant to tissue engineering and regenerative medicine. However, the precise function of S1P in dental pulp stem cells (DPSCs) and its osteogenic differentiation remains unclear. We here investigated the function of S1P/S1P receptor (S1PR)-mediated cellular signaling in the osteogenic differentiation of DPSCs and clarified the fundamental signaling pathway. Our results showed that S1P-treated DPSCs exhibited a low rate of differentiation toward the osteogenic phenotype in association with a marked reduction in osteogenesis-related gene expression and AKT activation. Of note, both S1PR1/S1PR3 and S1PR2 agonists significantly downregulated the expression of osteogenic genes and suppressed AKT activation, resulting in an attenuated osteogenic capacity of DPSCs. Most importantly, an AKT activator completely abrogated the S1P-mediated downregulation of osteoblastic markers and partially prevented S1P-mediated attenuation effects during osteogenesis. Intriguingly, the pro-inflammatory TNF-α cytokine promoted the infiltration of macrophages toward DPSCs and induced S1P production in both DPSCs and macrophages. Our findings indicate that the elevation of S1P under inflammatory conditions suppresses the osteogenic capacity of the DPSCs responsible for regenerative endodontics.
Effectiveness of medical nutrition therapy in adolescents with type 1 diabetes: a systematic review
Authors: Granado-Casas Minerva , Solà Ivan , Hernández Marta , Rojo-López Marina Idalia , Julve Josep , Mauricio Didac 
Published: April 2022

Abstract
BACKGROUND
Medical nutrition therapy (MNT) has an integral role in overall diabetes management. During adolescence, consideration of physiological and psychosocial changes is essential for implementing an optimal diabetes treatment.

OBJECTIVES
Our aim was to identify, summarize, and interpret the published literature about MNT in adolescents with type 1 diabetes.

METHODS
The Medline (PubMed) and EMBASE databases were searched from January 1959 to December 2021. The inclusion criteria were interventional studies with MNT in adolescents with type 1 diabetes with a disease duration over 1 year, including the following outcomes: dietary intake and daily eating patterns (assessed with validated tools, two or more 24 h dietary recall or 3-day dietary records), the diabetes self-management education and support (DSMES), glycemic control, lipid profile and body mass index (BMI). The exclusion criteria were studies without a control group (except for pre-post studies), the lack of randomization and those studies that assessed only a single nutrient, food or meal consumption, as well as reviews, and in-vitro/in-vivo studies. The risk of bias assessment was performed using the Cochrane risk-of-bias tool for randomized trials. A narrative synthesis was performed to present the results. The quality of evidence was assessed with the GRADE guidance.

RESULTS
From a total of 5377 records, 12 intervention studies (9 RCT and 3 pre-post intervention studies) were included. The data were assessed in order to perform a meta-analysis; however, the studies were too heterogeneous. The studies showed conflicting results about the effectiveness of MNT on dietary pattern, DSMES, glycemic control, lipid profile and BMI.

CONCLUSIONS
Clinical research studies on the effectiveness of MNT in adolescents with type 1 diabetes are scarce. The limited number of studies with a high risk of bias precludes establishing robust conclusions on this issue. Further research is warranted.
Cisplatin resistance can be curtailed by blunting Bnip3-mediated mitochondrial autophagy
Authors: Vianello Caterina , Cocetta Veronica , Catanzaro Daniela , Dorn Gerald W , De Milito Angelo , Rizzolio Flavio , Canzonieri Vincenzo , Cecchin Erika , Roncato Rossana , Toffoli Giuseppe , Quagliariello Vincenzo , Di Mauro Annabella , Losito Simona , Maurea Nicola , Scaffa Cono , Sales Gabriele , Scorrano Luca , Giacomello Marta , Montopoli Monica 
Published: April 2022

AbstractCisplatin (CDDP) is commonly used to treat a multitude of tumors including sarcomas, ovarian and cervical cancers. Despite recent investigations allowed to improve chemotherapy effectiveness, the molecular mechanisms underlying the development of CDDP resistance remain a major goal in cancer research. Here, we show that mitochondrial morphology and autophagy are altered in different CDDP resistant cancer cell lines. In CDDP resistant osteosarcoma and ovarian carcinoma, mitochondria are fragmented and closely juxtaposed to the endoplasmic reticulum; rates of mitophagy are also increased. Specifically, levels of the mitophagy receptor BNIP3 are higher both in resistant cells and in ovarian cancer patient samples resistant to platinum-based treatments. Genetic BNIP3 silencing or pharmacological inhibition of autophagosome formation re-sensitizes these cells to CDDP. Our study identifies inhibition of BNIP3-driven mitophagy as a potential therapeutic strategy to counteract CDDP resistance in ovarian carcinoma and osteosarcoma.
HJURP regulates cell proliferation and chemo-resistance via YAP1/NDRG1 transcriptional axis in triple-negative breast cancer
Authors: Mao Misha , Jia Yunlu , Chen Yongxia , Yang Jingjing , Xu Ling , Zhang Xun , Zhou Jichun , Li Zhaoqing , Chen Cong , Ju Siwei , Wang Linbo 
Published: April 2022

AbstractTriple-negative breast cancer is still a difficult point in clinical treatment at present, and a deep study of its pathogenesis has great clinical value. Therefore, our research mainly focuses on exploring the progression of triple-negative breast cancer and determines the important role of the HJURP/YAP1/NDRG1 transcriptional regulation axis in triple-negative breast cancer. We observed significantly increased HJURP expression levels in triple-negative breast cancer compared to other subtypes. HJURP could affect the level of ubiquitination modification of YAP1 protein and then regulate its downstream transcriptional activity. Mechanistically, we found that YAP1 positively regulates NDRG1 transcription by binding the promoter region of the NDRG1 gene. And HJURP/YAP1/NDRG1 axis could affect cell proliferation and chemotherapy sensitivity in triple-negative breast cancer. Taken together, these findings provide insights into the transcriptional regulation axis of HJURP/YAP1/NDRG1 in triple-negative breast cancer progression and therapeutic response.
Preparation of dual-cross network polymers by the knitting method and evaluation of their mechanical properties
Authors: Kawai Yusaku , Park Junsu , Ishii Yoshiki , Urakawa Osamu , Murayama Shunsuke , Ikura Ryohei , Osaki Motofumi , Ikemoto Yuka , Yamaguchi Hiroyasu , Harada Akira , Inoue Tadashi , Washizu Hitoshi , Matsuba Go , Takashima Yoshinori 
Published: April 2022

AbstractBulk copolymerization of alkyl acrylates and cyclodextrin (CD) host monomers produced a single movable cross-network (SC). The CD units acted as movable crosslinking points in the obtained SC elastomer. Introducing movable crosslinks into a poly(ethyl acrylate/butyl acrylate) copolymer resulted in good toughness (Gf) and stress dispersion. Here, to improve the Young’s modulus (E) and Gf of movable cross-network elastomers, the bulk copolymerization of liquid alkyl acrylate monomer swelling in SC gave another type of movable cross-network elastomer with penetrating polymers (SCPs). Moreover, the bulk copolymerization of alkyl acrylate and the CD monomer in the presence of SC resulted in dual cross-network (DC) elastomers. The Gf of the DC elastomer with a suitable weight % (wt%) of the secondary movable cross-network polymer was higher than those of the SCP or SC elastomers. The combination of suitable hydrophobicity and glass transition of the secondary network was important for improving Gf. Small-angle X-ray scattering (SAXS) indicated that the DC elastomers exhibited heterogeneity at the nanoscale. The DC elastomers showed a significantly broader relaxation time distribution than the SC and SCP elastomers. Thus, the nanoscale heterogeneity and broader relaxation time distribution were important to increase Gf. This method to fabricate SCP and DC elastomers with penetrating polymers would be applicable to improve the Gf of conventional polymeric materials.
Robust dual topological insulator phase in NaZnBi
Authors: Lee Hyunggeun , Kang Yoon-Gu , Jung Myung-Chul , Han Myung Joon , Chang Kee Joo 
Published: April 2022

AbstractTopological insulators are characterized by $${\Bbb Z}_2$$

Z

2

indices, and their metallic surface states are protected by time-reversal symmetry. In topological crystalline insulators, on the other hand, crystal symmetry plays a key role in protecting surface states. Therefore, perturbation breaking time-reversal or crystal symmetry induces a phase transition to a conventional band insulator. In dual topological insulators (DTIs), in which time-reversal and crystal symmetries coexist, the topological character is more robust against perturbation. Here, we propose that NaZnBi is a new DTI with $${\Bbb Z}_2$$

Z

2

invariants $$(\nu _0;\nu _1\nu _2\nu _3) = (1;000)$$

(

ν

0

;

ν

1

ν

2

ν

3

)

=

(

1
;
000

)

and odd mirror Chern numbers ±1. We find that the characteristic Dirac cone is preserved even if either time-reversal or mirror symmetry is broken, verifying the robustness of the DTI phase. Given that gapless surface states can be experimentally observable even under perturbations that break any lattice symmetry or time-reversal, NaZnBi can be a good candidate material for future device applications.
Supraphysiological activation of TAK1 promotes skeletal muscle growth and mitigates neurogenic atrophy
Authors: Roy Anirban , Kumar Ashok 
Published: April 2022

AbstractSkeletal muscle mass is regulated through coordinated activation of multiple signaling pathways. TAK1 signalosome has been found to be activated in various conditions of muscle atrophy and hypertrophy. However, the role and mechanisms by which TAK1 regulates skeletal muscle mass remain less understood. Here, we demonstrate that supraphysiological activation of TAK1 in skeletal muscle of adult mice stimulates translational machinery, protein synthesis, and myofiber growth. TAK1 causes phosphorylation of elongation initiation factor 4E (eIF4E) independent of mTOR. Inactivation of TAK1 disrupts neuromuscular junction morphology and causes deregulation of Smad signaling. Using genetic approaches, we demonstrate that TAK1 prevents excessive loss of muscle mass during denervation. TAK1 favors the nuclear translocation of Smad4 and cytoplasmic retention of Smad6. TAK1 is also required for the phosphorylation of eIF4E in denervated skeletal muscle. Collectively, our results demonstrate that TAK1 supports skeletal muscle growth and prevents neurogenic muscle atrophy in adult mice.
ZNF117 regulates glioblastoma stem cell differentiation towards oligodendroglial lineage
Authors: Liu Jun , Wang Xiaoying , Chen Ann T. , Gao Xingchun , Himes Benjamin T. , Zhang Hongyi , Chen Zeming , Wang Jianhui , Sheu Wendy C. , Deng Gang , Xiao Yang , Zou Pan , Zhang Shenqi , Liu Fuyao , Zhu Yong , Fan Rong , Patel Toral R. , Saltzman W. Mark , Zhou Jiangbing 
Published: April 2022

AbstractGlioblastoma (GBM) is a deadly disease without effective treatment. Because glioblastoma stem cells (GSCs) contribute to tumor resistance and recurrence, improved treatment of GBM can be achieved by eliminating GSCs through inducing their differentiation. Prior efforts have been focused on studying GSC differentiation towards the astroglial lineage. However, regulation of GSC differentiation towards the neuronal and oligodendroglial lineages is largely unknown. To identify genes that control GSC differentiation to all three lineages, we performed an image-based genome-wide RNAi screen, in combination with single-cell RNA sequencing, and identified ZNF117 as a major regulator of GSC differentiation. Using patient-derived GSC cultures, we show that ZNF117 controls GSC differentiation towards the oligodendroglial lineage via the Notch pathway. We demonstrate that ZNF117 is a promising target for GSC differentiation therapy through targeted delivery of CRISPR/Cas9 gene-editing nanoparticles. Our study suggests a direction to improve GBM treatment through differentiation of GSCs towards various lineages.
Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning
Authors: Schamber Michael R. , Vafabakhsh Reza 
Published: April 2022

AbstractTransfer of information across membranes is fundamental to the function of all organisms and is primarily initiated by transmembrane receptors. For many receptors, how ligand sensitivity is fine-tuned and how disease associated mutations modulate receptor conformation to allosterically affect receptor sensitivity are unknown. Here we map the activation of the calcium-sensing receptor (CaSR) - a dimeric class C G protein-coupled receptor (GPCR) and responsible for maintaining extracellular calcium in vertebrates. We show that CaSR undergoes unique conformational rearrangements compared to other class C GPCRs owing to specific structural features. Moreover, by analyzing disease associated mutations, we uncover a large permissiveness in the architecture of the extracellular domain of CaSR, with dynamics- and not specific receptor topology- determining the effect of a mutation. We show a structural hub at the dimer interface allosterically controls CaSR activation via focused electrostatic repulsion. Changes in the surface charge distribution of this hub, which is highly variable between organisms, finely tune CaSR sensitivity. This is potentially a general tuning mechanism for other dimeric receptors.
Chromatin profiling in human neurons reveals aberrant roles for histone acetylation and BET family proteins in schizophrenia
Authors: Farrelly Lorna A. , Zheng Shuangping , Schrode Nadine , Topol Aaron , Bhanu Natarajan V. , Bastle Ryan M. , Ramakrishnan Aarthi , Chan Jennifer C , Cetin Bulent , Flaherty Erin , Shen Li , Gleason Kelly , Tamminga Carol A. , Garcia Benjamin A. , Li Haitao , Brennand Kristen J. , Maze Ian 
Published: April 2022

AbstractSchizophrenia (SZ) is a psychiatric disorder with complex genetic risk dictated by interactions between hundreds of risk variants. Epigenetic factors, such as histone posttranslational modifications (PTMs), have been shown to play critical roles in many neurodevelopmental processes, and when perturbed may also contribute to the precipitation of disease. Here, we apply an unbiased proteomics approach to evaluate combinatorial histone PTMs in human induced pluripotent stem cell (hiPSC)-derived forebrain neurons from individuals with SZ. We observe hyperacetylation of H2A.Z and H4 in neurons derived from SZ cases, results that were confirmed in postmortem human brain. We demonstrate that the bromodomain and extraterminal (BET) protein, BRD4, is a bona fide ‘reader’ of H2A.Z acetylation, and further provide evidence that BET family protein inhibition ameliorates transcriptional abnormalities in patient-derived neurons. Thus, treatments aimed at alleviating BET protein interactions with hyperacetylated histones may aid in the prevention or treatment of SZ.
Bifunctional zeolites-silver catalyst enabled tandem oxidation of formaldehyde at low temperatures
Authors: Li Na , Huang Bin , Dong Xue , Luo Jinsong , Wang Yi , Wang Hui , Miao Dengyun , Pan Yang , Jiao Feng , Xiao Jianping , Qu Zhenping 
Published: April 2022

AbstractBifunctional catalysts with tandem processes have achieved great success in a wide range of important catalytic processes, however, this concept has hardly been applied in the elimination of volatile organic compounds. Herein, we designed a tandem bifunctional Zeolites-Silver catalyst that enormously boosted formaldehyde oxidation at low temperatures, and formaldehyde conversion increased by 50 times (100%versus2%) at 70 °C compared to that of monofunctional supported silver catalyst. This is enabled by designing a bifunctional catalyst composed of acidic ZSM-5 zeolite and silver component, which provides two types of active sites with complementary functions. Detached acidic ZSM-5 activates formaldehyde to generate gaseous intermediates of methyl formate, which is more easily oxidized by subsequent silver component. We anticipate that the findings here will open up a new avenue for the development of formaldehyde oxidation technologies, and also provide guidance for designing efficient catalysts in a series of oxidation reactions.
Spillover effects of food recalls: A milk recall scenario experiment in China
Authors: Hao Na , Zhang Yi , Zheng Qiujie , Wetzstein Michael 
Published: April 2022

AbstractFood recall is a major ingredient in food safety with existing literature focusing mainly on its direct impacts. Few studies focus on possible spillover effects. It is hypothesized that food recalls have a spillover effect on the recalled brand and purchase channel. As a test of this hypothesis, a 2-purchase channel by 3-recall strategy scenario experiment was conducted on spillover effects of a milk recall in Beijing, China. The results indicate that food-safety scares have significant negative impacts on consumers’ purchase intention on the recalled brand and purchase channel, and the impacts are more significant for online than offline marketing. However, voluntary recalls by online firms help mitigate these negative effects and restore consumers’ purchase intention more than offline voluntary recalls. An online food incident creates an issue of trust toward general online platforms. Online vendors should take greater care in guaranteeing food safety and actively take restorative actions such as voluntary recalls after a food safety incident. Results provide empirical evidence for industry organizations and governments to stipulate a strict food safety and incident resolution system for e-commerce.
Expanding energy envelope in holographic display via mutually coherent multi-directional illumination
Authors: Lee Dukho , Bang Kiseung , Nam Seung-Woo , Lee Byounghyo , Kim Dongyeon , Lee Byoungho 
Published: April 2022

AbstractHolographic display is considered as the most promising three-dimensional (3D) display due to its unique feature of reconstructing arbitrary wavefronts. However, the limited étendue, which hinders the immersive experience of observers, remains a major unresolved issue in holographic display technique. In this paper, we propose a novel approach to tweak the constraints of étendue by expanding the energy envelope in holographic display via mutually coherent multi-illumination. The proposed concept contains both a light source design for generating a mutually coherent multi-directional wave and a computer-generated hologram optimization framework for providing high-resolution 3D holograms. To verify the proposed approach, a benchtop prototype of a holographic near-eye display providing an intrinsic large exit-pupil is implemented. The experimental results clearly show that the exit-pupil is effectively expanded by four times and an appropriate viewpoint image is reconstructed according to the view position.
Clinical study of skill assessment based on time sequential measurement changes
Authors: Yamaguchi Tomoko , Nakamura Ryoichi , Kuboki Akihito , Otori Nobuyoshi 
Published: April 2022

AbstractEndoscopic sinus surgery is a common procedure for chronic sinusitis; however, complications have been reported in some cases. Improving surgical outcomes requires an improvement in a surgeon’s skills. In this study, we used surgical workflow analysis to automatically extract “errors,” indicating whether there was a large difference in the comparative evaluation of procedures performed by experts and residents. First, we quantified surgical features using surgical log data, which contained surgical instrument information (e.g., tip position) and time stamp. Second, we created a surgical process model (SPM), which represents the temporal transition of the surgical features. Finally, we identified technical issues by creating an expert standard SPM and comparing it to the novice SPM. We verified the performance of our methods by using the clinical data of 39 patients. In total, 303 portions were detected as an error, and they were classified into six categories. Three risky operations were overlooked, and there were 11 overdetected errors. We noted that most errors detected by our method involved dangers. The implementation of our methods of automatic improvement points detection may be advantageous. Our methods may help reduce the time for reviewing and improving the surgical technique efficiently.