bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of hepatocarcinogenesis by transcriptome and exome sequencing via chronic HBV X expression and aflatoxin B1 exposure in immortal hepatic cell
Authors: Wang Xiang-Kun , Zhou Xin , Yu Ting-Dong , Han Chuang-Ye , Zhu Guang-Zhi , Qin Wei , Huang Jian-Lu , Liao Xi-Wen , Chen Zi-Jun , Yang Cheng-Kun , Ye Xin-Ping , Peng Tao 
Published: June 2022

Abstract
Backgrounds:
Hepatitis B virus (HBV) and aflatoxin B1 (AFB1) exposure have synergistic effects on the occurrence and progression of hepatocellular carcinoma. In this study, we constructed HBV X (HBX) expression and AFB1 exposure models promoting hepatocarcinogenesis.
Methods
The HBX-overexpressing HL-7702 cell model was constructed by lentivirus transfection. In vitro experiments and transcriptome sequencing determined HBX functions and transcriptional traits. An interventional system containing AFB1, NAPDH, liver microsomes and medium was constructed to mimic AFB1 exposure. Transcriptome and exome sequencing identified transcriptional traits and alterations in exon regions. Differentially expressed (differential) RNAs involved in HBX overexpression, AFB1 exposure and the AFB1 + HBX exposure groups were validated by real-time polymerase chain reaction assays.
Results
HBX overexpressing showed decreased capacity for cellular migration, proliferation, invasion and clonal formation compared with control cells (P ≤ 0.05). Mutational “fingerprints” of AFB1 (TP53-R249S, AGG > AGT) and novel mutations at codons 247, 249 and 250 were unveiled in the model. Transcriptome sequencing identified the following differential RNAs: 285 mRNAs, 9 miRNAs, 78 long non-coding RNAs (lncRNAs) and 117 circular RNAs (circRNAs) in HBX group; 585 mRNAs, 143 miRNAs, 208 lncRNAs and 58 circRNAs in the AFB1 group; and 564 mRNAs, 126 miRNAs, 233 lncRNAs and 61 circRNAs in the AFB1 + HBX group. Enrichment analysis suggested the involvement of differential RNAs in angiogenesis, oxidative phosphorylation and ribosome pathways, etc. Additionally, competing endogenous RNA networks of differential mRNA-miRNA-lncRNA/circRNA cross-talk were constructed in the above groups. Exome sequencing unveiled 1061 (AFB1) and 3601 (AFB1 + HBX) single nucleotide variations, 30 (AFB1) and 40 (AFB1 + HBX) insertion and deletion alterations, 1068 (AFB1) and 138719 (AFB1 + HBX) copy number variations. The number of alterations was consistently higher in the AFB1 + HBX group compared to the AFB1 group.
Conclusions
We successfully constructed a cellular model mimicking HBX expression, AFB1 exposure and provided novel insights for understanding AFB1/HBX-related hepatocarcinogenesis.
Fractional Sumudu decomposition method for time-fractional OBBM-Burgers equation with its similarity reduction, conservation laws, and analytical solutions
Authors: AL-Denari Rasha B. , Ahmed Engy. A. , Moawad S. M. , EL-Kalaawy O. H. 
Published: June 2022

Abstract
The time-fractional Oskolkov-Benjamin-Bona-Mahony-Burgers (TF-OBBMB) equation is investigated in this paper. For this equation, we apply the Lie symmetry analysis to detect the symmetries and the vector fields provided using the definition of Riemann-Liouville (R-L) fractional derivatives. These symmetries allow us to construct the similarity reduction for the considered equation which converts it to a fractional ordinary differential (FOD) equation. Add to that, a set of solutions for the TF-OBBMB equation is obtained by the fractional sub-equation method. Also, we build a numerical solution by using the fractional Sumudu decomposition method in the sense of Caputo fractional derivatives accompanied by the absolute errors and the effect of the fractional-order α. Furthermore, we present a clear explanation for the physical meaning of both analytical and numerical solutions. Finally, we compute the conservation laws in detail in the light of the new conservation theorem.
The impact of antimicrobial management by clinical pharmacists in obstetrics and gynecology on antimicrobial indicators, bacterial resistance and drug costs from 2011 to 2021: a retrospective observational study
Authors: Jin Jing , Zhu JiaLei , Tang Jing 
Published: June 2022

Abstract
Objective: Based on the current situation of the antibiotic application in China, the clinical pharmacists continuously strengthen antibiotics application, aiming to evaluate the effectiveness and role of clinical pharmacists in antibiotics management through retrospective research from 2011 to 2021.Methods: We set up a team composed of pharmacists to carry out multi angle intervention measures, such as setting up working group, formulating plan, setting up management rules through the pre-trial system, prescription comments, jointing supervision with administrative department, carrying out training and publicity, etc. We analyzed antibiotics use, evaluated the changes of bacterial drug resistance, and counted antibiotics cost.Results: Through the pharmacists' intervention and rectification of unreasonable antibacterial drug orders, the rational utilization rate of antibiotics were significantly increased, and the cost of antibiotics was reduced. The use rate of antibiotics in clean surgery decreased from 90.22% to 11.14%, the use rate of antibiotics decreased from 63.82% to 30.26%, and the use intensity decreased from 42.75DDDs to 30.04DDDs. The varieties, time and course of antibacterials used in wards were improved to varying degrees. Bacterial drug resistance improved distinctly, such as the resistance of Escherichia coli to cephalosporins, ciprofloxacin and carbapenems decreased in varying degrees. The amount of antibacterial drugs decreased significantly.Conclusion: It is feasible and effective for pharmacists in gynecology and obstetrics to control the use of antibiotics, which plays a positive role in promoting the safe, effective and economic application of antibiotics, and provides a valuable reference on the management of antibiotics in domestic.
BVFLEMR: An Integrated Federated Learning and Blockchain Technology for Cloud-based Medical Records Recommendation System
Authors: Hai Tao , Zhou Jincheng , Srivid S.R , Jain Sanjiv Kumar , Young Praise , Agrawal Shweta 
Published: June 2022

Abstract
Blockchain is the latest boon in the world which handles mainly banking and finance. The blockchain is also used in the healthcare management system for the effective maintenance of electronic health and medical records. The technology ensures security, privacy, and immutability. Federated Learning is a revolutionary learning technique in deep learning, which supports learning from the distributed environment. This work proposes a framework by integrating the blockchain and Federated Deep Learning in order to provide a tailored recommendation system. The work focuses on two modules of blockchain-based storage for electronic health records, where the blockchain uses a Hyperledger fabric and is capable of continuously monitoring and tracking the updates in the Electronic Health Records in the cloud server. In the second module, LightGBM and N-Gram models are used in the collaborative learning module to recommend a tailored treatment for the patient’s cloud-based database after analyzing the EHR. The work shows good accuracy. Several metrics like precision, recall, and F1 scores are measured showing its effective utilization in the cloud database security.
Reducing the Scarcity of Fuel Wood by Utilizing Agro and Forestry Waste
Authors: Balamurugan T. , C Ezhilarasan , I Duraimurugan , K Prakash 
Published: June 2022

Abstract
The secure and efficient discarding of agricultural wastes is a main concern today in India. A possible solution for this waste disposal is to convert these agricultural wastes into highly compressed briquettes fuel. This research article elaborates an experimental investigation of fuel characteristics of bio-briquettes prepared by combining the palm flower with other bio wastes such as teak wood leaf, rice bran, wheat husk, tamarind shell, mango leaf and ground nut shell separately and paraffin wax as binding material. Briquettes have been made by combining 50% of palm flower with 40% of other bio wastes and 10 % of paraffin wax as binding material. Furthermore, the proximate analysis was conducted to estimate the presence of moisture, volatile matter, ash, fixed carbon along with calorific values of the biomass briquettes prepared. The results concluded that the composite biomass briquettes showed enhanced combustion properties when compared with that of different waste material. Biomass in its actual form with lower bulk density resulted in increased transportation and storage cost and it is not possible to use as it is as an efficient flammable fuel. Compacting biomass wastes under briquetting technology highly supports to minimize the above such issues and improved the combustion characteristics of the fuel which in turn may contribute to overcome the future energy crises due to the fast depletion of conventional fossil fuel.
First in-situ evidence of solar wind acceleration
Authors: Cid Consuelo , Larrodera Carlos , Flores-Soriano Manuel 
Published: June 2022

Abstract
Physical processes involved in the acceleration of stellar winds are essential in astrophysics as such winds are a mass loss process for stars. Hypothesis for stellar winds are difficult to check with experimental data in any other star but the Sun and even the processes of solar wind dynamics are poorly understood. Since the proposal by Parker of the stationary expansion of the solar atmosphere, the existence of the solar wind has been extensively demonstrated from data acquired from different missions in the Heliosphere. However, in-situ data showing that solar wind accelerates with the distance were still missing. Here we show the evolution of the solar wind speed distribution function with the heliocentric distance from 16 R⊙ to 172 R⊙ using Parker Solar Probe data. We observe that solar wind is accelerated until at least 60 R⊙, more than 20 R⊙ away than expected from previous studies. Below ∼ 25 R⊙ the subalfvenic wind dominates and is negligible from 35 R⊙ onwards. Here we find that the slow and fast winds are as mixed in the distribution function below 25 R⊙ as at 1 AU. Our findings provide empirical support for the models of solar wind dynamics.
Inter-rater reliability of the extended Composite Quality Score (CQS-2) - Protocol
Authors: Mickenautsch Steffen , Rupf Stefan , Miletić Ivana , Yengopal Veerasamy 
Published: June 2022

Abstract
Objectives: The aim of this study is to establish the inter-rater reliability of the CQS-2 and to test the null-hypotheses that it does not differ significantly from that of the first CQS version (CQS-1). Methods: Four independent raters will be selected to rate 45 clinical trial reports using CQS-1 and CQS-2. The raters will remain unaware regarding each other’s participation in this study until all rating is completed. Each rater will receive only one rating-template at a time in a random sequence for CQS-1 and CQS-2 rating. Raters will complete each template and send these back to the principal investigator via email. Each rater will receive her/his next template two weeks after submission of the completed previous template. The inter-rater reliabilities for the overall appraisal score of the CQS-1 and the CQS-2 will be established by use of the Brennan-Prediger coefficient. The coefficients of both CQS versions will be compared by use of the two-sample z-test. All data analysis will be carried out using SAS statistical software. A 5% significance level will be used. During secondary analysis, we will establish the inter-rater reliability for each single criterion and each corroboration level for both CQS versions.Reporting: The results of this study will be reported in line with the Guidelines for Reporting Reliability and Agreement Studies (GRAAS). The final report will be made available online as preprint in one of the major preprint repositories and submitted to a peer-reviewed journal.
Stress May Not Impair Higher Cognitive Functions Via Depleted Cognitive Resources
Authors: Qiu Boyu , Liu Ting , Chen Yanrong , He Xu , Zhou Cui , Zhang Wei 
Published: June 2022

Abstract
Whether stress impairs available cognitive resources and affects multiple cognitive functions is under debate. The current study used a relatively direct measure of available cognitive resources (i.e., the cognitive control capacity) to test the effect of acute stress and found that cognitive control capacity did not differ between conditions (stress and control) by using both the p-value and Bayes factor as indicators. We argue that stress might not impair higher cognitive functions through depleted cognitive resources after checking the effectiveness of the manipulations. The results potentially contribute to understanding the psychological consequence of stress-related disorders.
The Potential of Naturally Occurring Antibiotic Peptides to from Coiled-Coils
Authors: Wade David 
Published: June 2022

Abstract:
The amino acid sequences of 183 naturally occurring, antibiotic peptides were analyzed for the presence of characteristic features associated with the coiled-coil motif. Most sequences contained some of these features, but no single peptide contained all of them. The results indicate that these peptides probably cannot aggregate as coiled-coils, and they have implications for models of the mechanisms of antibiotic action.
Isolation and in vitro Cleavage Specificities of Epidermal Growth Factor Binding Proteins
Authors: Beg Obaid U. , Uddin Mukarram 
Published: June 2022

Abstract:
Results of the present investigation indicate the presence of three mouse epidermal growth factor binding proteins (EGF-BP-A, -Band -C) in BPN strain of mice . All of these proteases show specificity towards Arg­ Val and Tyr-Ser bonds which appears, inpart, to be dependent on adjacent amino acids.Additionally EGF-BP­ A also cleaves Lys-Val and Leu-Ser bonds; EGF-BP-B cleaves Lys-Ala bond and EGF-BP-C cleaves Lys-Val and Lys-Aia bonds. These cleavages distingui sh EGF-BP's from each other.
Stimulation of Non-Specific Resistance by Human Casein Fragment (54-59) and its Synthetic Analogues against Leishmania Donovani Infection+.
Authors: Sharma P. , Anuradha  , Sharan R. , Haq W. , Kundu B. , Katiyar J.C. , Mathur K.B. 
Published: June 2022

Abstract:
The prophylactic effect of human β casein fragment (54-59) and its synthetic congeners has been studied against L. donovani infection in hamsters. Maximum parasite inhibition (84%) was observed with compound 4. The activity of this compound was further confirmed in vitro
Conformational Behaviour of an Analogue of the S-Peptide: A Molecular Dynamics Study
Authors: Abruscato Vincenzo , Ranghino Graziella , Maria Villa Anna 
Published: June 2022

Abstract:
The S-peptide, derived from the hydrolysis of Ribonuclease, is a stable a.-helix in water, although it is only 20 amino acids long. In view of increasing the helical content and stability, a mutated sequence has been proposed. The present work aims to shed light on the conformational preferences and on the helix stability of the proposed sequence by means of Molecular Dynamics simulations performed with different procedures.
Three-dimensional Self-organization of Tetraphenylporphyrin and its Composites with C60: Structure and Optical Properties
Authors: Elistratova Marina , Romanov Nikolay , Zakharova Irina 
Published: June 2022

Background:
Thin films of a 5,10,15,20-tetraphenylporphyrin (H2TPP) and its metal
complexes (MeTPP, where Me = Co, Cu, Zn, Fe-Cl) were obtained by vacuum thermal evaporation
under quasi-equilibrium conditions (hot wall method) and by coating from a toluene solution
on various substrates. It was shown that H2TPP, CuTPP and CoTPP have an ability to selforganization
into linear structures during crystallization (nanowhiskers, nanowires, or nanorods),
regardless of our obtaining method.

Methods:
FeClTPP and ZnTPP crystallize into planar films with the same preparation technique.
Co-deposition with fullerene C60 allows obtaining fullerene crystallites on the surface of
tetraphenylporphyrin, subsequently creating the so-called bulk heterojunctions.

Results:
The possibility of controlling the shape of obtained crystallites by changing technological
parameters and substrates has been shown for metal free tetraphenylporphyrin. High degree
crystalline ZnTPP and H2TPP films are characterized by a red shift of photoluminescence and
absorption spectra under crystallization.

Conclusion:
A change in the relative intensity of the photoluminescence spectrum maxima of
various nature is observed during transition from amorphous to crystalline structure of H2TPP.
Role of the Gut Microbiome in Diabetes and Cardiovascular Diseases
Including Restoration and Targeting Approaches- A Review
Authors: Ahuja Alka , UM Dhanalekshmi , MP Saraswathy , S Nandakumar , F Arul Prakash , KN Gurpreet 
Published: June 2022

Abstract:
Metabolic diseases, including cardiovascular diseases (CVD) and diabetes, have become the leading cause of morbidity and mortality worldwide. Gut microbiota appears to play a
vital role in human disease and health, according to recent scientific reports. The gut microbiota
plays an important role in sustaining host physiology and homeostasis by creating a cross-talk
between the host and microbiome via metabolites obtained from the host's diet. Drug developers
and clinicians rely heavily on therapies that target the microbiota in the management of metabolic diseases, and the gut microbiota is considered the biggest immune organ in the human
body. They are highly associated with intestinal immunity and systemic metabolic disorders like
CVD and diabetes and are reflected as potential therapeutic targets for the management of metabolic diseases. This review discusses the mechanism and interrelation between the gut microbiome and metabolic disorders. It also highlights the role of the gut microbiome and microbially
derived metabolites in the pathophysiological effects related to CVD and diabetes. It also spotlights the reasons that lead to alterations of microbiota composition and the prominence of gut
microbiota restoration and targeting approaches as effective treatment strategies in diabetes and
CVD. Future research should focus onunderstanding the functional level of some specific microbial pathways that help maintain physiological homeostasis, multi-omics, and develop novel
therapeutic strategies that intervene with the gut microbiome for the prevention of CVD and
diabetes that contribute to a patient's well-being.
Metodología para medir la confiabilidad en líneas de ensamble
Authors: Orrantia Daniel Gilberto , Sánchez Leal Jaime , De la Riva Rodríguez Jorge , Reyes Martínez Rosa María , Herrera Ríos Ericka Berenice 
Published: June 2022

La medición es un aspecto clave para evaluar el resultado de cualquier proceso. Las líneas de ensamble deben medirse considerando la confiabilidad tanto del producto como del proceso de producción. El propósito de este estudio fue desarrollar una metodología que permitiera conocer el estado de una línea de ensamble y que integrara un modelo de medición para obtener un índice de confiabilidad para una línea de este tipo de la industria automotriz. Los dos productos resultantes de este estudio fueron la metodología con su estructura en etapas y el modelo matemático. La metodología está compuesta de cinco etapas y el modelo de cuatro elementos. La confiabilidad pudo ser calculada para una línea de ensamble de la industria automotriz haciendo uso de la media de la distribución de mejor ajuste. Lo anterior permitió detectar el índice que más afectó a la confiabilidad general. El índice que más afectó al valor de confiabilidad general fue el de disponibilidad con 75.55 %, seguido del índice de eficiencia con 87.25 %, continuando con el índice de calidad con 90.15 % y, por último, con el menor efecto, está el índice de capacidad de entrega con 97.75 %.
An analysis of Satire Delivered by Politic Observer Rocky Gerung in Indonesia Lawyer Club (ILC)
Authors: Andry Rafi , Syarif Hermawati 
Published: June 2022

In delivering critics, the use of satire is indispensable so that the critics can be accepted or heard, because satire is very much used as a tool to convey the critics. This study aims to analyze the types and techniques of satire introduced by Abeshaus (2018) and Luke Endley (2017). The method used in this research is descriptive method. There are two problems in this study, (1) what types and (2) technique of the satire used by Rocky Gerung in his speech. The results show that in 24 techniques of satire there are 14 techniques of satire with Inflation (38.9%), Hyperbole (14.9%) and Innuendo (14.9 %) being the highest found and from 3 types of satire were found that Juvenalian (57.3%) as the dominant type. The result of this research is that Rocky Gerung tends to use critics by using Inflation with exaggerating the mistakes and circumstances. Regarding the type, the most commonly type found in this study is the Juvenalian type. Because Rocky Gerung tends to use the type of sarcasm that is harsh and reproachful
Implementasi Program Laskar Nagari Peduli Pangan dan Gizi (LNPPG) dalam Pencegahan Stunting di Kabupaten Padang Pariaman
Authors: Yasri Atika , Yusran Ramadhani 
Published: June 2022

This research was conducted in Padang Pariaman Regency wit theaimofdescribing the implementationof the Laskar Nagari Program for Food and Nutrition Care (LNPPG) in the Prevention of Stunting. Thisstudy uses qualitativeapproach withdescriptivemethods. The research location is in the Padang Pariaman Regency. The research informants were determined by purposive sampling. Data collection techniques are carried out through interviews, observations, and documentation studies and data reduction is carried out so that accurate conclusions can be drawn from the data that has been obtained. The results of this study prove that the implementation of the LNPPG Program in Stunting Prevention is carried out in the form of counseling from the health department to the community, establishing health posts, providing additional food. However, the implementation is still experiencing various obstacles so that it has not been able to achieve the expected goals. For example, the level of community participation is low, this is due to the difficulty of the community understanding the importance of this program, the low consistency of cadres / implementers in doing their work, the credibility / quality of cadres / implementors is not all good even though there are guidance and training, and the minimum budget available and inadequate facilities, aswellasthedifficulty of accessing the location and the terrain that is difficult to pass when conducting this LNPPG program extension. This problem has resulted in the LNPPG Program's achievements not being optimally realized.
Implementasi Kebijakan Pencegahan Covid-19 di Kota Padang
Authors: Bayu Firdaus Arya , Yusran Ramadhani 
Published: June 2022

Covid-19 is a novel disease that is spread by viruses in the Corona virus family, most notably SARS-CoV-2, or Corona Virus.The main idea of this study is to ascertain the effectiveness of government interventions aimed at avoiding COVID-19.This study combines qualitative and descriptive methodologies.The investigation was conducted in Padang City, West Sumatra Province.Observation, interviewing, and documentation studies are all methods of data collection.Meanwhile, this research employs reduction of the data, display of data, and the conclusion making methodologies.This research findings indicate that the Padang City government's efforts to address community needs and resolve community problems, most notably through the issuance of Perwako policy No. 32 of 2020 in Padang City, demonstrate the government's commitment to resolving social problems and national non-natural disasters, particularly in the Padang city area.Thus, the government's efforts to avoid COVID-19 in connection with the Perwako policy are considered to be proceeding smoothly and successfully.