bluuebunny/crossref_metadata_2025_split huggingface dataset:
Berberine Suppressed the Progression of Human Glioma Cells by Inhibiting the TGF-β1/SMAD2/3 Signaling Pathway
Authors: Jin Yun , Zhang Jiawei , Pan Yunfeng , Shen Wangzhen 
Published: October 2022

Background: Previous studies have shown that berberine can inhibit glioma progression, although the underlying molecular mechanisms needed to be explored further. The aim of this study was to evaluate the suppressive effects of berberine on human glioma cells, and identify the underlying signaling pathways. Material and Methods: The cytotoxic effect of different concentrations of berberine against normal human glial cells (HEB) and 4 glioma cell lines was evaluated by the CCK-8 assay. Apoptosis was assayed by flow cytometry. In vitro migration and invasion were analyzed by the wound healing and transwell assays. The expression levels of specific proteins were measured by western blotting and ELISA. Results: Berberine significantly inhibited the proliferation of human glioma U-87 cells, and induced apoptosis in the U-87 and LN229 cells by downregulating Bcl-2, and upregulating Bax and caspase-3. In addition, berberine also inhibited migration and invasion of the glioma cells. Furthermore, berberine exerted its effects on the proliferation, migration, invasion, and apoptosis of glioma cells by inhibiting the TGF-β1/SMAD2/3 signaling pathway, and exogenous TGF-β abrogated the pro-apoptotic and anti-oncogenic effects of berberine. Conclusions: Berberine inhibits glioma progression by targeting the TGF-β1/SMAD2/3 signaling pathway.
Individual and contextual factors in the Swedish Nutrition Care Process Terminology implementation
Authors: Lövestam Elin , Orrevall Ylva , Boström Anne-Marie 
Published: October 2022

Background Standardised terminologies and classification systems play an increasingly important role in the continuous work towards high quality patient care. Currently, a standardised terminology for nutrition care, the Nutrition Care Process (NCP) Terminology (NCPT), is being implemented across the world, with terms for four steps: Nutrition Assessment (NA), Nutrition Diagnosis (ND), Nutrition Intervention (NI) and Nutrition Monitoring and Evaluation (NME). Objective To explore associations between individual and contextual factors and implementation of a standardised NCPT among Swedish dietitians. Method A survey was completed by 226 dietitians, focussing on: (a) NCPT implementation level; (b) individual factors; and (c) contextual factors. Associations between these factors were explored through a two-block logistic regression analysis. Results Contextual factors such as intention from management to implement the NCPT (OR (odds ratio) ND 15.0, 95% Confidence Interval (CI) 3.9–57.4, NME 3.7, 95% CI 1.1–13.0) and electronic health record (EHR) headings from the NCPT (OR NI 3.6, 95% CI 1.4–10.7, NME 3.8, 95% CI 1.1–11.5) were associated with higher implementation. A positive attitude towards the NCPT (model 1 OR ND 3.8, 95% CI 1.5–9.8, model 2 OR ND 5.0, 95% CI 1.4–17.8) was also associated with higher implementation, while other individual factors showed less association. Conclusion Contextual factors such as intention from management, EHR structure, and pre-defined terms and headings are key to implementation of a standardised terminology for nutrition and dietetic care. Implications for practice Clinical leadership and technological solutions should be considered key areas in future NCPT implementation strategies.
Factors associated with uncontrolled asthma among adult asthmatic patients in eastern Ethiopia: A multicenter study
Authors: Heluf Helina , Assefa Nega , Dessie Yadeta , Goshu Abel Tibebu , Fekadu Gelana , Abdisa Lemesa , Tamiru Dawit 
Published: October 2022

Objective: Asthma is a major public health problem worldwide. Despite various attempts, it is still uncontrolled in most parts of the world. Moreover, it is contributing to the national and global burden of non-communicable diseases. Studying factors associated with uncontrolled asthma in different parts of Ethiopia is crucial to control the disease and improving the quality of life of asthmatic patients. Thus, this study aimed to determine the factors associated with uncontrolled asthma among adult asthmatic patients in Eastern Ethiopia. Methods: Facility-based cross-sectional study was employed from 1st October 2020 to 30th January 2021. A total of 416 adult asthmatic patients participated in the study from six hospitals follow-up clinics. Asthma control test was used to assess the participants level of asthma control and a score of ⩽19 were regarded to have uncontrolled asthma. Data were analyzed using SPSS version 24. Bivariable and multivariable analyses were carried out to identify factors associated with uncontrolled asthma and variables with a p value of less than 0.05 were considered statistically significant. Result: The prevalence of uncontrolled asthma was 66.1 % (95% confidence interval: 61.5–70.4). Not attending scheduled medical follow-up (adjusted odds ratio: 2.54; 95% confidence interval: 1.28–4.99), poor knowledge about asthma (adjusted odds ratio = 4.59; 95% confidence interval: 2.01–10.51), negative attitude toward asthma (adjusted odds ratio = 3.72; 95% confidence interval: 1.83–7.59), and poor adherence to medications (adjusted odds ratio = 2.53; 95% confidence interval: 1.25–5.13) were significantly associated with uncontrolled asthma. Conclusion: In this study, the prevalence of uncontrolled asthma was considerably high. Not attending scheduled medical follow-up, poor knowledge about asthma, negative attitude toward asthma, and poor adherence to anti-asthma medications were associated with uncontrolled asthma. Therefore, it is crucial to focus on increasing the patients’ level of awareness about asthma control, improving medication adherence, and avoiding triggering factors.
Determining laboratory parameters in pregnant women with severe COVID-19
Authors: Djusad Suskhan , Irwinda Rima , Harzif Achmad Kemal , Surya Raymond , Wibowo Noroyono , Saroyo Yudianto Budi , Adjie JM Seno 
Published: October 2022

Objective: Coronavirus disease causes a wide spectrum of clinical picture in pregnant women. There are still a few studies concerning laboratory parameters to differentiate the severity of coronavirus disease-19 in pregnant patients. This article aims to identify the cut-off on laboratory parameters between pregnant women with asymptomatic/mild/moderate and severe/critical coronavirus disease-19 illness. Methods: All coronavirus disease-19 pregnant women coming to Dr. Cipto Mangunkusumo hospital between January and August 2021 were recruited into this cross-sectional study. All data extracted from medical records were classified into demographic characteristics and laboratory parameters. We defined demographic characteristics as age, parity, systolic, and diastolic blood pressure, heart rate, temperature, respiratory rate, and oxygen saturation. Laboratory parameters measured in this study consisted of complete blood count, renal function test including urea, creatinine, liver function test including aspartate transferase, alanine transferase, infection marker test including procalcitonin, C-reactive protein, interleukin-6, cycle threshold values, ferritin, coagulation test including d-dimer, fibrinogen, random blood glucose, albumin, electrolytes. Data analysis was performed using SPSS statistics. Results: There were 159 cases assessed in this study consisting of 130 (81.8%) patients with asymptomatic/mild/moderate illness and 29 (18.2%) patients with severe/critical illness of coronavirus disease-19. Of 28 laboratory parameters, 12 of them were significant statistically; thus, it resulted to make a cut-off using receiver operating characteristic curve and show relative risk, and 95% confidence interval. In multivariate analysis that aspartate transferase, D-dimer, and albumin were laboratory parameters impacted to the severity of coronavirus disease-19 in pregnant women. The area under receiver operating characteristic curve was 0.834 (95% CI 0.736–0.932). Conclusion: Of all laboratory parameters, urea, aspartate transferase, alanine transferase, procalcitonin, C-reactive protein, lactate dehydrogenase (LDH), ferritin, D-dimer, random blood glucose, cycle threshold values, albumin, and chloride differs between asymptomatic/mild/moderate, and severe/critical illness coronavirus disease-19 in pregnant women; aspartate transferase, D-dimer, and albumin were laboratory parameters impacted the most to the severity of coronavirus disease-19 in pregnant women.
Emergence of colistin-resistant gram-negative rods in intensive care units: A cross-sectional study from a developing country
Authors: Syed Beenish , Ishaque Sadia , Imran Abira , Muslim Osaid , Khalid Seema , Siddiqui Abdul Basit 
Published: October 2022

Objectives: To determine the clinical features, outcomes, and factors associated with the emergence of colistin-resistant gram-negative rods isolated from patients admitted to intensive care units. Methods: This cross-sectional study was conducted at the intensive care units of Liaquat National Hospital, from April 2019 to February 2020. Gram-negative rods resistant to colistin with minimum inhibitory concentrations ⩾ 4 mcg/mL according to Clinical and Laboratory Standards Institute criteria as reported in cultures were included. Clinical, demographical data and treatment given were recorded and analyzed using SPSS version 25. Results: A total of 93 patients were included; 58.1% were males. The mean age of patients was 59.48 ± 18.36 years. The most common organism isolated was Klebsiella pneumoniae (91.4%). The most common specimen was the tracheal (62.4%). Ventilator-acquired pneumonia was seen in 38.7%. The most common co-morbid disease seen in patients was diabetes (41%); 77% had a symptomatic infection and were treated with a combination of 2 or more antibiotics, most commonly meropenem plus fosfomycin. The most common susceptible antibiotics were fosfomycin (72%) and tigecycline (50.5%). Mean intensive care unit stay and total duration of hospital stay were prolonged (16.83 ± 12.93 and 23.34 ± 17.52 days, respectively). Forty-eight (62.3%) patients with symptomatic infection with colistin-resistant isolates were treated and discharged, and mortality was seen in 23 (29.9%). A significant association was found between mortality and symptomatic infection, endotracheal intubation with mechanical ventilation (p = 0.003), and a prolonged hospital stay of >20 days (p = 0.041). Conclusion: Colistin-resistant gram-negative rods pose a significant problem especially in developing countries because of limited therapeutic options. Stringent infection control and comprehensive antimicrobial stewardship programs are needed to overcome this challenge.
Exploring the sociocultural contexts in which healthcare staff respond to and use online patient feedback in practice: In-depth case studies of three NHS Trusts
Authors: Ramsey Lauren , Lawton Rebecca , Sheard Laura , O’Hara Jane 
Published: October 2022

Objectives Patients are increasingly reporting about their healthcare experiences online and NHS Trusts are adopting different approaches to responding. However, the sociocultural contexts underpinning these organisational approaches remain unclear. Therefore, we aimed to explore the sociocultural contexts underpinning three organisations who adopted different approaches to responding to online patient feedback. Methods Recruitment of three NHS Trusts was theoretically guided, and determined based on their different approaches to responding to online patient feedback (a nonresponding organisation, a generic responding organisation and an organisation providing transparent, conversational responses). Ethnographic methods were used during a year of fieldwork involving staff interviews, observations of practice and documentary analysis. Three in-depth case studies are presented. Findings The first organisation did not respond to or use online patient feedback as staff were busy firefighting volumes of concerns received in other ways. The second organisation adopted a generic responding style due to resource constraints, fears of public engagement and focus on resolving known issues raised via more traditional feedback sources. The final organisation provided transparent, conversational responses to patients online and described a 10-year journey enabling their desired culture to be embedded. Conclusions We identified a range of barriers facing organisations who ignore or provide generic responses to patient feedback online. We also demonstrated the sociocultural context in which online interactions between staff and patients can be embraced to inform improvement. However, this represented a slow and difficult organisational journey. Further research is needed to better establish how organisations can recognise and overcome barriers to engaging with online patient feedback, and at pace.
Use of Medicinal Plants by Cancer Patients Under Chemotherapy in the Northwest of Morocco (Rabat Area) : Cross-Sectional Study
Authors: El Orfi Nadia , Boutayeb Saber , Haddou Rahou Bouchra , Errihani Hassan 
Published: October 2022

Background Variety of conventional treatments are used to treat cancer. Cancer patients adopt other alternative therapies including medicinal plants. Their curative power results in the presence of secondary metabolites in its different parts. However, they can have toxic effects and interactions with conventional treatment and even chemosensitivity of the cancer cells. Objectives This study aims to determine the prevalence of the use of medecinal plants by cancer patients undergoing chemotherapy, list the medecinal plants used, identify the most consumed, present the reported adverse effects and determine the predictive factors of their use. Materials and Methods This was a cross-sectional study of 203 patients followed at the National Institute of Oncology in Rabat from 01 October 2018 to 30 November 2018. Regarding socio-demographic and clinical characteristics and data on the use of medicinal plants were collected from a questionnaire. Findings of 203 patients, 37% used medicinal plants. 30 plants also the honey were identified during this study. The “euphorbia honey"was consumed at (40%), The most used plants were garlic (13%), turmeric, fenugreek and thyme (11% each). 5% of patients presented side effects related to the consumption of medicinal plants. There is a significant association between the use of medicinal plants and socio-economic level (p  =  0.004) and duration of illness (p  =  0.048). Conclusion This study revealed a high prevalence of medicinal plants used by cancer patients receiving chemotherapy at National Institute of Oncology. The more clinical studies are desirable to demonstrate the efficacy of medicinal plants and their therapeutic effects to encourage their consumption or prohibit them.
Identification of miRNA biomarkers for breast cancer by combining ensemble regularized multinomial logistic regression and Cox regression
Authors: Li Juntao , Zhang Hongmei , Gao Fugen 
Published: October 2022

Abstract
Background
Breast cancer is one of the most common cancers in women. It is necessary to classify breast cancer subtypes because different subtypes need specific treatment. Identifying biomarkers and classifying breast cancer subtypes is essential for developing appropriate treatment methods for patients. MiRNAs can be easily detected in tumor biopsy and play an inhibitory or promoting role in breast cancer, which are considered promising biomarkers for distinguishing subtypes.

Results
A new method combing ensemble regularized multinomial logistic regression and Cox regression was proposed for identifying miRNA biomarkers in breast cancer. After adopting stratified sampling and bootstrap sampling, the most suitable sample subset for miRNA feature screening was determined via ensemble 100 regularized multinomial logistic regression models. 124 miRNAs that participated in the classification of at least 3 subtypes and appeared at least 50 times in 100 integrations were screened as features. 22 miRNAs from the proposed feature set were further identified as the biomarkers for breast cancer by using Cox regression based on survival analysis. The accuracy of 5 methods on the proposed feature set was significantly higher than on the other two feature sets. The results of 7 biological analyses illustrated the rationality of the identified biomarkers.

Conclusions
The screened features can better distinguish breast cancer subtypes. Notably, the genes and proteins related to the proposed 22 miRNAs were considered oncogenes or inhibitors of breast cancer. 9 of the 22 miRNAs have been proved to be markers of breast cancer. Therefore, our results can be considered in future related research.
Renal–hepatic–pancreatic dysplasia-1 with a novel NPHP3 genotype: a case report and review of the literature
Authors: Zhu Hui , Zhao Zhi-hui , Zhu Shu-yao , Xiong Fu , He Li-hong , Zhang Yong , Wang Jin 
Published: October 2022

Abstract
Background
Renal–hepatic–pancreatic dysplasia type 1 (RHPD1) is a rare sporadic and autosomal recessive disorder with unknown incidence. RHPD1 is caused by biallelic pathogenic variants in NPHP3, which encode nephrocystin, an important component of the ciliary protein complex.

Case presentation
In this case report, we describe a male newborn who was confirmed by ultrasound to have renal enlargement with multiple cysts, pancreatic enlargement with cysts, and increased liver echogenicity, leading to the clinical diagnosis of RHPD. In addition, a compound heterozygous pathogenic variant, namely, NPHP3 c.1761G > A (p. W587*) and the c.69delC (p. Gly24Ala24*11) variant, was detected by WES. The patient was clinically and genetically diagnosed with RHPD1. At 34 h of life, the infant died of respiratory insufficiency.

Conclusion
This is the first published case of RHPD1 in China. This study broadens the known range of RHPD1 due to NPHP3 pathogenic variants.
Evaluation of large airway specimens obtained by transbronchial lung cryobiopsy in diffuse parenchymal lung diseases
Authors: Sato S. , Yamakawa H. , Takemura T. , Nakamura T. , Nishizawa T. , Oba T. , Kawabe R. , Akasaka K. , Amano M. , Matsushima H. 
Published: October 2022

Abstract
Background
The difference in diagnostic yield between surgical lung biopsy and transbronchial lung cryobiopsy (TBLC) in diffuse parenchymal lung diseases (DPLD) has been reported to be due to differences in the rate of interpathologist agreement, specimen size, and specimen adequacy. In TBLC, the specimens containing large airway components are generally believed as inadequate specimens for histological evaluation, but the detailed characteristics of TBLC specimens including the large airway and the impact on histological diagnostic rates of DPLD have not been investigated.

Methods
We retrospectively reviewed the specimen characteristics of patients with DPLD who underwent TBLC.

Results
Between February 2018 and January 2020, 74 patients and 177 specimens were included. There were 85 (48.0%) large airway specimens (LAS) that contained bronchial gland or bronchial cartilage. The ideal specimen ratio was significantly lower in the LAS-positive group than that in the LAS-negative group (5.8% vs. 45.6%), and the proportion of bronchioles, alveoli, and perilobular area were similarly lower in the LAS-positive group. The presence of traction bronchiectasis and diaphragm overlap sign on high-resolution computed tomography (HRCT) were also significantly higher in the LAS-positive group than those in the LAS-negative group. We observed a statistically significant trend in histological diagnostic yield (40.7% in LAS positive group; 60.8% in LAS positive and negative group; 91.6% in LAS negative group) (Cochran-Armitage trend test).

Conclusion
LAS is a specimen often collected in TBLC and contains a low percentage of bronchioles, alveoli, and perilobular area. Since the histological diagnostic yield tends to be higher in cases that do not contain LAS, it may be important to determine the biopsy site that reduces the frequency of LAS collection by referring to the HRCT findings in TBLC.
Global well-posedness and large time behavior of epitaxy thin film growth model
Authors: Duan Ning , Yuan Shuang 
Published: October 2022

AbstractWe consider the global well-posedness and large time behavior of solutions for epitaxy thin film growth model in $\mathbb{R}^{d}$

R
d

with the dimensional $d\geq 3$
d
≥
3
. First, using the pure energy method and a standard continuity argument, we prove that there exists a unique global strong solution under the condition that the initial data is sufficiently small. Moreover, we also establish the suitable negative Sobolev norm estimates and obtain the optimal decay rates of the higher-order spatial derivatives of the strong solution.
Continuous estimation of coseismic and early postseismic slip phenomena via the GNSS carrier phase to fault slip approach: a case study of the 2011 Tohoku-Oki sequence
Authors: Tanaka Yusuke , Ohta Yusaku , Miyazaki Shin’ichi 
Published: October 2022

AbstractThe transition process from coseismic to early postseismic phenomena within a half-day remains a significant topic for understanding the slip budget and friction properties of the fault. However, the investigation of this phase has undergone limited advancement. This is mainly due to the lack of precision pertaining to the Global Navigation Satellite System (GNSS) analysis caused by difficulty of separation between fault slip and other unknown parameters such as tropospheric delay. Therefore, we propose an alternative approach (phase-to-slip; PTS) that detects fault slip directly from GNSS carrier phase variation without conventional positioning. Since the PTS simultaneously estimates fault slip and other unknowns, we can quantitatively evaluate their separation accuracy and contribution in the estimation. This study attempts to continuously estimate the coseismic and early postseismic slip of the 2011 Tohoku-Oki sequence using the PTS method. We analyzed 1-Hz carrier phase data for approximately 2 h before and after the mainshock origin (2011/03/11/05:46UTC). As a result, we successfully obtained the coseismic slip of the Mw9.0 mainshock and two major aftershocks in the off-Ibaraki (Mw7.8) and off Iwate (Mw7.4) regions. For all three events, the estimated slip distribution, equivalent moment magnitude, and calculated displacement field well agreed with those from the conventional positioning. Additionally, our results suggest postseismic slip mainly in the downdip area adjacent to the mainshock rupture. We obtained three major slip areas around the downdip region near Aomori, Iwate, and Miyagi. The estimated slip amount reaches approximately 0.1–0.2 m in 34 min immediately after the mainshock. The equivalent seismic moment of the slip areas near Iwate and Miyagi were approximately Mw7.4. This amount is similar or slightly greater than the estimations based on conventional positioning. Significant overlap is observed in the locations of the slip areas. In this manner, PTS continuously detected a series of coseismic and early postseismic slips with timescale ranging from a few minutes to an hour. Our results demonstrate the capability of the PTS method for broadband fault slip monitoring and its potential contribution to the investigation of early postseismic phenomena.