bluuebunny/crossref_metadata_2025_split huggingface dataset:
DoE Engineered Development and Validation of an RP-HPLC Method for Simultaneous Estimation of Temozolomide and Resveratrol in Nanostructured Lipid Carrier
Authors: Mittal Saurabh , Ali Javed , Baboota Sanjula 
Published: April 2022

Abstract

Background
Temozolomide is drug of choice for the treatment of glioblastoma, but dose-related side effects limit its use. Resveratrol suppresses tumor growth and promotes apoptosis. Many studies showed synergistic activity of resveratrol and temozolomide against glioblastoma. There are methods reported for the assessment of temozolomide and resveratrol individually, but no analytical method has been reported for assessment of temozolomide and resveratrol simultaneously.

Objective
Therefore, the present study aimed to develop and optimize an HPLC analytical method for the simultaneous assessment of temozolomide and resveratrol in a developed nanostructured lipid carrier.

Method
A Central composite rotable design was used to optimize the method. The method was developed using a C18 column. The composition of the mobile phase was 30% methanol and 70% glacial acetic acid (0.1% v/v in HPLC grade water); detecting wavelength was 310 nm. Forced degradation test was also performed to demonstrate the proposed HPLC method’s ability to indicate stability.

Results
The LOD for temozolomide and resveratrol was found to be 1.10 and 0.83 µg/mL, respectively, while LOQ was 3.33 and 2.52 µg/mL, respectively. The drug loading and entrapment efficacy of the formulation, as determined using the aforementioned method, was found to be 6.73 and 96.28% for temozolomide and 3.45 and 89.39% for resveratrol, respectively.

Conclusions
The developed HPLC method was simple, rapid, economical, precise, accurate, and reproducible, and it had high selectivity with good detection limits. Standard guidelines of ICH Q2 (R1) including linearity, specificity, system suitability, robustness, precision, accuracy, the LOQ, and LOD gave satisfactory results. Forced degradation studies showed a good stability-indicating capacity of the developed HPLC method.

Highlights
Analytical Quality by Design is a powerful tool that could be used for the development of the analytical method. Central composite rotable design was used for optimizing the method. The percent of methanol and concentration of glacial acetic acid were selected as two independent variables for optimization.
Maternal immune activation and dietary soy isoflavone supplementation influence pig immune function but not muscle fiber formation
Authors: Bryan Erin E , Chen Xuenan , Smith Brooke Nicole , Dilger Ryan Neil , Dilger Anna C 
Published: April 2022

AbstractThe goals of this study were to determine the impact of maternal PRRSV infection on offspring muscle and immune development and the potential of dietary soy isoflavones to mitigate those effects. Thirteen first-parity gilts (“gilts”) were randomly allotted into one of three treatments: not infected and fed a diet devoid of isoflavones (CON), infected with porcine reproductive and respiratory syndrome virus (PRRSV) and fed the control diet (POS) or that supplemented with 1,500 mg/kg soy-derived isoflavones (ISF). Gilts were inoculated with PRRSV intranasally on gestational day (GD) 70. After farrowing (GD 114 ± 2), 1–2 offspring (“pigs”) closest to the average litter weight were selected either at birth (3 ± 2 d of age) or weaning (21 ± 2 d of age) to determine body, muscle, and organ weights as well as muscle cell number and size. Four weaned pigs of average body weight within each litter were selected for postnatal immune challenge. At PND 52, pigs were injected with 5 µg/kg BW lipopolysaccharide (LPS) intraperitoneally. Serum was collected at 0, 4, and 8 h following LPS administration to analyze tumor necrosis factor alpha (TNF-α). At PND 59, pigs were administered a novel vaccine to elicit an adaptive immune response. At PND 59, 66, and 73, peripheral blood mononuclear cells were isolated and T-cell populations determined by flow cytometry. Both POS and ISF pigs exhibited persistent PRRSV infections throughout the study (PND 1-73). At PND 3, whole body, muscle, and organ weights were not different (P &gt; 0.22) between groups, with the exception of relative liver weight, which was increased (P &lt; 0.05) in POS compared with CON pigs. At PND 21, ISF pigs had reduced (P ≤ 0.05) whole body and muscle weights, but greater (P &lt; 0.05) kidney weight compared with CON, and greater (P &lt; 0.05) relative liver weight compared with CON and POS. Muscle fiber number and size were not different (P &gt; 0.39) between groups at birth or weaning. After LPS administration, TNF-α was greatest in ISF pigs (P &lt; 0.05) at both 0 and 8 h post-challenge. At the peak time-point of 4 h post-challenge, ISF pigs had the greatest concentration of TNF-α and CON pigs had the lowest, with POS pigs being intermediate (P = 0.01). After vaccination, ISF offspring had shifts in T-cell populations indicating an impaired immune response. These data indicate that maternal PRRSV infection may impact offspring organ growth and immune function, particularly when the dam is supplemented with isoflavones.
The effects of creep feed composition and form and nursery diet complexity on small intestinal morphology and jejunal mucosa-specific enzyme activities after weaning in pigs
Authors: Christensen Brenda , Huber Lee-Anne 
Published: April 2022

Abstract
Fifty-six litters from first-parity sows standardized to 12 piglets were used to determine the effects of creep feed composition and form and the provision of low- or high-complexity nursery diets on the evolution of small intestinal histomorphology and jejunal mucosa-specific enzyme activities postweaning. At 5 d of age, litters (initial bodyweight [BW] 2.31 ± 0.61 kg) were assigned to one of four creep feeding regimens (n = 14): 1) commercial creep feed (COM), 2) liquid milk replacer (LMR), 3) pelleted milk replacer (PMR), or 4) no creep feed (NO). At weaning (21 d of age), six pigs per litter were provided a HIGH- (contained highly digestible animal proteins) or LOW- (contained corn and soybean meal as main protein sources) complexity nursery diet (n = 7). At 21, 28, and 59 d of age, two pigs per pen (one castrated male and one female) were euthanized, and ileal and jejunal segments for histomorphological measurements and jejunal mucosal scrapings were collected to determine specific mucosa enzyme activities. At weaning, pigs provided COM had a greater ileal absorptive capacity (M) than LMR or NO, which were not different (14.1 vs. 10.4 and 10.5 ± 0.9 μm2; P &lt; 0.05); PMR was intermediate. On days 28 and 59, M was not different among pigs regardless of creep feed treatments. Pigs fed LOW had reduced jejunal villus height (VH; P &lt; 0.001) and M (P &lt; 0.001) on day 28 vs. day 21. The VH and M were not different for pigs fed HIGH or LOW by the end of the nursery period. For all dietary treatments except COM-HIGH and COM-LOW, jejunal mucosal maltase-specific activity was not different between days 21 and 28 of age but greater on day 59 (P &lt; 0.05). For pigs that received COM-HIGH, maltase-specific activity was not different between days 21 and 28 but greater on day 59 than day 28 (P &lt; 0.05). For pigs that received COM-LOW, maltase-specific activity was not different between days 21, 28, and 59. Regardless of creep or nursery treatment, sucrase-specific activity was the greatest on day 59, followed by days 21 and 28 (P &lt; 0.001), and lactase-specific activity was greater on day 21 than on days 28 and 59 (P &lt; 0.001), which were not different. Therefore, pigs that provided LOW diet had greater villus atrophy and reduced M during the first week after weaning vs. pigs that provided HIGH, regardless of creep feeding regimen, but were able to recover by the end of the nursery period.
Classification and distribution of evergreen broad-leaved forests in Jiangxi, East China
Authors: Liu Yizhen , Shen Haihua , Ge Gang , Xing Aijun , Tang Zhiyao , Fang Jingyun 
Published: April 2022

AbstractEvergreen broad-leaved forest (EBLF) is climax vegetation in East China, and Jiangxi Province is located at the center of EBLF. Although many phytosociological studies have provided detailed descriptions on this forest at local and regional scales, knowledge on the high-level classification of EBLF and factors controlling their tree species composition and distribution are still lacking. We aim at providing a high-level classification framework for EBLF in Jiangxi and exploring the roles of topographic and climatic factors in driving their distributions. We investigated 205 EBLF plots covering the main alliances across Jiangxi Province. Two-way indicator species analysis (TWINSPAN) and detrended correspondence analysis (DCA) were used to distinguish the EBLF types. Diagnostic and indicator species were detected. The nonparametric Kruskal–Wallis test was used to compare the differences between the groups of EBLFs. We also used redundancy analysis and variation partitioning to estimate the effects of climatic, geographical and topographical factors on the distribution of EBLFs in Jiangxi. The EBLFs in Jiangxi were classified into four types based on the diagnostic taxa and verified by TWINSPAN, i.e. lowland, ravine monsoon, montane typical and dwarf cloud EBLF types. These four EBLF types are associated with climatic and topographic conditions, and their distribution pattern reflects biogeographic differences. Our study provides an important basis for the syntaxonomic classification of EBLFs and further in-depth studies on humid EBLFs in East China. We also emphasize the importance of conservation practices to protect these forests as diversified Tertiary relict plants.
Cutaneous fistula on the cheek associated with oculo-auriculo-vertebral spectrum
Authors: Nishimoto Soh , Kawai Kenichiro , Fujiwara Toshihiro , Ishise Hisako , Kakibuchi Masao 
Published: April 2022

Abstract
A Japanese boy, presented with epibulbar dermoid and ipsilateral preauricular appendages, had a pit on his cheek of the same side. An atrial septal defect and vertebral fusions were also identified. He was diagnosed with a mild type of oculo-auriculo-vertebral spectrum (OAVS). At the age of 18 months, his cheek was swollen with a slight fever. An infected cyst and cutaneous fistula enveloped by the risorius muscle were extracted. It was assumed to be a remnant of the fissure between the maxillary and mandibular prominences. This was the first case of cutaneous fistula confirmed histologically with OAVS, although there seem to be more cases. The possibility of the mechanism of smiling cheek dimple is also discussed.
Veteran Suicide: Missed Opportunities for Suicide Prevention Within Concealed Pistol License Curriculum
Authors: Consolino Tara A , Yarvis Jeffrey S 
Published: April 2022

ABSTRACT

Introduction
Veteran suicide is a growing topic for public health concern. Despite enhancements from the Department of Veterans Health Administration, clinicians continue to struggle to identify and engage veterans at risk for suicide.

Materials and Methods
Examining suicide prevention efforts, epidemiology, risk factors, and barriers to care; Substance Abuse and Mental Health Services Administration and the U.S. Department of Veterans Affairs Governor’s Challenges identified lethal means as one of 3 priority areas to focus on in during this agenda.

Results
The writers identified gaps within the current literature as an area for future research on lethal means within the veteran population and opportunity for intervention with concealed pistol license curriculum.

Conclusion
This article ends with recommendations for further research on veteran suicides, acquisition of concealed pistol licenses, and advocacy for legislative change to require suicide prevention resources and education to be included in the training curriculum for concealed pistol license holders.
The extragalactic γ-ray background: imprints from the physical properties and evolution of star-forming galaxy populations
Authors: Owen Ellis R , Kong Albert K H , Lee Khee-Gan 
Published: April 2022

ABSTRACT
Star-forming galaxies (SFGs) harbour an abundant reservoir of cosmic rays (CRs). At GeV energies, these CRs undergo interactions with their environment to produce γ-rays, and the unresolved γ-ray emission from populations of SFGs form a component of the isotropic extragalactic γ-ray background (EGB). In this work, we investigate the contribution to the 0.01–50 GeV EGB from SFG populations located up to redshift z = 3. We find this is dominated by starbursts, while the contribution from main-sequence SFGs is marginal at all energies. We also demonstrate that most of the γ-ray contribution from SFGs emanates from low-mass galaxies, with over 80 per cent of the emission originating from galaxies with stellar masses below $10^8 \, {\rm M}_{\odot }$. Many of these galaxies are located at relatively high redshift, with their peak EGB contribution arising ∼700 Myr before the noon of cosmic star formation. We find that the precise redshift distributions of EGB sources at different energies imprint intensity signatures at different angular scales, which may allow their contribution to be distinguished using analyses of small-scale EGB intensity anisotropies, particularly if the diffuse EGB is dominated by hadronic CR-driven γ-ray emission from SFGs. We show that the EGB is sensitive to the evolution of low-mass populations of galaxies, particularly around z ∼ 2.5, and that it provides a new means to probe the engagement of CRs in these galaxies before the high noon of cosmic star formation.
Anti-neutrophil cytoplasmic antibody-associated vasculitis complicated by thrombotic microangiopathy with posterior reversible encephalopathy syndrome successfully treated with eculizumab: A case report
Authors: Kitamura Fumiya , Yamaguchi Makoto , Nishimura Minori , Katsuno Takayuki , Ito Mayumi , Sugiyama Hirokazu , Iwagaitsu Shiho , Nobata Hironobu , Kinashi Hiroshi , Ishimoto Takuji , Banno Shogo , Ito Yasuhiko 
Published: April 2022

ABSTRACT
Thrombotic microangiopathy is characterised by endothelial cell injury, intravascular platelet-fibrin thrombi, and vascular damage, leading to acute kidney injury, thrombocytopenia, and microangiopathic haemolytic anaemia. Among the autoimmune diseases related to thrombotic microangiopathy, anti-neutrophil cytoplasmic antibody-associated vasculitis-related thrombotic microangiopathy cases have been rarely reported; therefore, the optimal treatment for associated vasculitis-related thrombotic microangiopathy remains unknown. An 84-year-old woman without significant medical history presented with a 1-month history of general fatigue, fever, and deteriorating bilateral leg numbness and was admitted to our hospital. She had elevated myeloperoxidase anti-neutrophil cytoplasmic antibody levels, polyneuropathy, and rapid progressive glomerulonephritis because of pauci-immune crescentic glomerulonephritis, as revealed by a kidney biopsy. Accordingly, we diagnosed her with microscopic polyangiitis. After administering methylprednisolone pulse therapy, rituximab, and intravenous immunoglobulin, the patient’s mental state deteriorated, presenting signs of thrombotic microangiopathy with posterior reversible encephalopathy syndrome. Intermittent haemodialysis and plasma exchange were initiated; however, her condition did not improve, and eculizumab administration was initiated thereafter. The patient’s symptoms showed a remarkable response to eculizumab; thrombotic microangiopathy findings, kidney function, and neurological symptoms improved after only two doses of eculizumab, and she achieved sustained remission. The extremely effective course of eculizumab treatment indicated that overt complement activation affected the development of thrombotic microangiopathy. Anti-neutrophil cytoplasmic antibody-associated vasculitis-related thrombotic microangiopathy may be mediated by complement activation, and prompt induction of eculizumab therapy may be a superior strategy to prevent organ damage. Further studies should elucidate the role of complement activation in associated vasculitis-related thrombotic microangiopathy and the efficacy of eculizumab treatment.
Follow-up Blood Culture Practices for Gram-Negative Bloodstream Infections in Immunocompromised Hosts at a Large Academic Medical Center
Authors: Buzzalino Lauren Groft , Mease James , Bernhardi Ciera L , Bork Jacqueline T , Johnson J Kristie , Claeys Kimberly C 
Published: April 2022

Abstract
The role of follow-up blood cultures (FUBCs) in gram-negative bloodstream infections to improve clinical outcomes remains controversial, especially among immunocompromised patients. Among 139 patients, FUBCs were common (117, 84.2%); however, positive FUBCs were rare (3, 2.6%). Only presence of fever was associated with a positive FUBC.
SARS-CoV-2 mRNA Vaccine Effectiveness in Health Care Workers by Dosing Interval and Time Since Vaccination: Test-Negative Design, British Columbia, Canada
Authors: El Adam Shiraz , Zou Macy , Kim Shinhye , Henry Bonnie , Krajden Mel , Skowronski Danuta M 
Published: April 2022

Abstract

Background
One- and two-dose mRNA vaccine effectiveness (VE) estimates against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection by dosing interval and time since vaccination were assessed among healthcare workers (HCWs) in publicly funded acute and community (nonresidential) healthcare facilities in British Columbia, Canada.

Methods
A test-negative design was used with controls matched to cases (6:1) on epidemiological week of SARS-CoV-2 test date. mRNA vaccination was defined by receipt of the first dose ≥21 days or second dose ≥14 days before the test date. HCWs ≥18 years old tested for SARS-CoV-2 between epi-weeks 3 and 39 (January 17–October 2, 2021) were included, when varying dosing intervals and a mix of circulating variants of concern contributed, including Delta dominance provincially from epi-week 31 (August 1).

Results
Single- and two-dose analyses included 1265 and 1246 cases, respectively. The median follow-up period (interquartile range) was 49 (34–69) days for single-dose and 89 (61–123) days for two-dose recipients, with 12%, 31%, and 58% of second doses given 3–5, 6, or ≥7 weeks after the first. Adjusted mRNA VE against SARS-CoV-2 was 71% (95% CI, 66%–76%) for one dose and 90% (95% CI, 88%–92%) for two doses, similar to two heterologous mRNA doses (92%; 95% CI, 86%–95%). Two-dose VE remained &gt;80% at ≥28 weeks post–second dose. Two-dose VE was consistently 5%–7% higher with a ≥7-week vs 3–5-week interval between doses, but with overlapping confidence intervals.

Conclusions
Among HCWs, we report substantial single-dose and strong and sustained two-dose mRNA vaccine protection, with the latter maintained for at least 7 months. These findings support a longer interval between doses, with global health and equity implications.
The Value of a Longitudinal Human Immunodeficiency Virus Track for Medical Students: 10-Year Program Evaluation
Authors: Marcelin Jasmine R , Brosnihan Paul , Swindells Susan , Fadul Nada , Bares Sara H 
Published: April 2022

Abstract
We surveyed graduates of a longitudinal medical school human immunodeficiency virus curriculum to evaluate its impact. Respondents felt comfortable caring for people with human immunodeficiency virus (PWH) and found value from the curriculum regardless of ultimate career path. Programs like this contribute to the development of culturally sensitive clinicians comfortable caring for PWH.
Navigating Human Immunodeficiency Virus Screening Recommendations for People on Pre-Exposure Prophylaxis and the Need to Update Testing Algorithms
Authors: Meyerowitz Eric A , Bernardo Raffaele M , Collins-Ogle Michelle D , Czeresnia Jonathan M , Matos Cariane M , Mullis Caroline , Root Heather B , Torres-Isasiga Julián A , Tsai Helen , Zingman Barry S 
Published: April 2022

Abstract
Incident HIV infections occurring in people on PrEP may have delayed seroconversion. New CDC guidelines recommend the addition of HIV-1 viral load for screening for all on PrEP. We believe antigen/antibody screening should continue for tenofovir-based PrEP at this time.
W 49 N MCN-a: A disk-accreting massive protostar embedded in an early-phase hot molecular core
Authors: Miyawaki Ryosuke , Hayashi Masahiko , Hasegawa Tetsuo 
Published: April 2022

Abstract
We present ALMA archival data for 219–235 GHz continuum and line observations toward the hot molecular core (HMC) W 49 N MCN-a (UCHII region J1) at a resolution of ∼${0.^{\prime \prime }3}$. The dust continuum emission, showing an elongated structure of ${1.^{\prime \prime }40} \times {0.^{\prime \prime }95}$ (position angle = 43.°5) perpendicular to the outflow seen in SiO and SO, represents a rotating flattened envelope, or torus, with a radius of 7800 au inclined at 47.°5 or larger. The emissions from CH3CN, 13CS, HNCO, HC3N, SO2, DCN, H2CO, OCS, CH3OH, and C18O exhibit a consistent velocity gradient as a result of rotation. The magnitude of each velocity gradient is different, reflecting that each line samples a specific radial region. This allows us to derive a rotation curve as Vrot ∝ R0.44 ± 0.11 for 2400 au ≲  R  ≲ 14000 au, giving the dynamical mass as $M_{\rm dyn} = 57.0^{+24.5}_{-17.1}\, (R\, [{\rm au}]/3000)^{1.88}\, M_{\odot }$. The envelope mass independently estimated from the dust emission is 910 M⊙ (for Tdust = 180 K) for R ≤ 7800 au and 32 M⊙ (for Tdust = 300 K) for R ≤ 1700 au. The dynamical mass formula agrees well with these mass estimates within an uncertainty of a factor of three in the latter. The envelope is self-gravitating and is unstable to form spiral arms and fragments, allowing rapid accretion to the inner radii with a rate of the order of 10−2 M⊙ yr−1, although inward motion was not detected. The envelope may become a non-self-gravitating Keplerian disk at R  ≲  (300–1000) au. The formula is also consistent with the total mass ∼104 M⊙ of the entire HMC 0.15 pc (31000 au) in radius. Multiple transitions of CH3CN, HNCO, and CH3OH provide rotation temperatures of 278$^{+39}_{-30}$, 297$^{+52}_{-39}$, and 154$^{+73}_{-37}$ K, respectively, for R  ≲  1700 au, suggesting that the central source of MCN-a has an intrinsic bolometric luminosity of ∼106 L⊙. These results have revealed the structure and kinematics of MCN-a at its intermediate radii. With no broad-line H30α emission detected, MCN-a may be in the earliest phase of massive star formation.