bluuebunny/crossref_metadata_2025_split huggingface dataset:
Has the construction of National Independent Innovation Demonstration Zones reduced the urban carbon emissions? A quasi-natural experiment in China
Authors: Fang Lan , Tang Heyan , Mou Muge 
Published: April 2022

Abstract
This paper investigates the effects of innovation-driven development on carbon emissions reduction in Chinese urban area. We use panel data on 285 cities which covers 95.96% of Chinese cities during the period of 2003 to 2017. The time-varying difference-in-differences (DID) model is employed to estimate the impact of the creation of China’s National Independent Innovation Demonstration Zones (NIDZs) on urban carbon emission levels. Compared with non-pilot cities, our results indicate that total carbon dioxide emissions and emission intensity of pilot cities decreased by 4.8% and 5.8% respectively. The results suggest that the construction of NIDZs can significantly promote urban carbon emission reductions. Moreover, our mechanism tests indicate that this pilot policy can encourage technological innovation and optimize industrial structure, which in turn suppresses urban carbon dioxide emissions. Furthermore, heterogeneity analysis shows that the policy effect is stronger in cities with higher levels of human capital, government finance and information infrastructure. Overall, this study enriches the relevant research on carbon emission reduction of cities and puts forward some policy recommendations.
Synthesis, antimicrobial and antioxidant activity of triazole, pyrazole containing thiazole Derivatives and Molecular docking studies on COVID-19
Authors: Raghavender Matta , Pochampally Jalapathi , Dhoddi Bala Narsimha , Shankar Bhookya , Bitla Sampath , Akkiraju Anjini Gayatri 
Published: April 2022

Abstract
An innovative heterocyclic biologically active series of triazole, pyrazole contain 2,4-disubstituted thiazole analogs 12a-l derived from p-hydroxy benzaldehyde and phenylhydrazine in excellent yields and purity. All the synthesized compounds were unambiguously identified on the basis of their spectral data analyses (IR, 1H-NMR, 13C-NMR spectra, and HRMS). The final derivatives were evaluated for their in vitro anti-microbial activity after thorough purification. Among all tested compounds the compound 12e, 12f and 12k possess highest growth inhibitory activity at MIC values of 4.8, 5.1 and 4.0 μg/ml respectively. The antioxidant properties of these compounds demonstrated and revealed that remarkable activity compared to the standard antioxidant by using DPPH free radical-scavenging assay. Moreover, molecular docking studies to evaluate the probable interactions with the catalytic domain of the gram-positive S. Aureus topoisomerase IV enzyme may provide new insights for developing these new hybrids as potential antimicrobial agents. The binding affinities of compounds 12a-l were ranging from -10.0 to -11.0K.cal/mol with topoisomerase IV enzyme and with COVID-19 main protease binding affinities are ranging from-8.2 to-9.3 Kcal/mol. These docking studies reveal that the compounds 12a-l could be the best inhibitors for novel SARS Cov-2 virus and have more future in discovery of potent drug candidate.
Bulk Photovoltaic Behavior Induced in Mechanically Distorted MoTe2
Authors: Aftab Sikandar , Shehzad Muhammad Arslan , Kabir Fahmid , Ajmal Hafiz Muhammad Salman , Elahi Ehsan , Yousuf Saqlain , Iqbal Muhammad Waqas 
Published: April 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Safety of same-day discharge posterior lumbar decompression and/or discectomy: An observational study using administrative data from England
Authors: Gray William K , Day Jamie , Briggs Tim W. R. , Hutton Mike 
Published: April 2022

Abstract
Purpose
Same-day discharge is widely used in many surgical specialities. If carefully planned, it can improve patient outcomes whilst using resources efficiently. We aimed to investigate the safety of same-day discharge following a posterior lumbar decompression and/or discectomy (PLDD).
Methods
This was a retrospective analysis of administrative data. We extracted data form the Hospital Episodes Statistics database for the five years from 1st April 2014 to 31st March 2019. Patients undergoing an elective one or two level PLDD aged 19–54 years during the index stay were included. The primary exposure variable was same-day discharge or post-surgery overnight stay and the primary outcome was emergency hospital readmission within 90 days post-discharge.
Results
Data were available for 45,814 PLDD performed across 100 hospital trusts of which 7,914 (17.3%) were performed as same-day discharge. Same-day discharge rates varied from 87.7–0% across hospital trusts. Fourteen (15.6%) trusts had same-day discharge rates above 30% and 57 (63.3%) trusts had same-day discharge rats below 10%. The odds of emergency hospital readmission within 90 days were lower for same-day discharge patients (odds ratio 0.72 (95% confidence interval 0.61 to 0.85). There was no difference in outcomes for patients seen at trusts with a same-day discharge rate of ≥ 30% compared to trusts with a same-day discharge rate of ≤ 10%.
Conclusions
Based on our analysis, same-day discharge low-complexity elective PLDD is safe in patients below the age of 55 years. There is potential for many providers to substantially increase their rates of same-day discharge.
Transcriptome-based Identification and Molecular Evolution of Cytochrome P450 Genes and Expression Profiling during sycamore lace bug Corythucha ciliata Feeding on London planetree Platanus acerifolia
Authors: Tusun Abudurusuli , Li Fengqi , Dewer Youssef , wu chunyan 
Published: April 2022

Abstract
Background Plant cytochrome P450s are important in the biosynthesis of essential physiological compounds. However, the gene family of P450s in london planetree have not yet been reported.Objective To explore the sequence characteristics and expression pattern of P450s in london planetree.Methods The phylogenetic studies, evolutionary analysis,gene expression profiling, Weighted correlation network analysis of P450 genes in london planetree were conducted. The jasmonic acid pathway key synthetase gene AOS was analyzed by qRT-PCR, cloned, homology modeled, and molecular docking analysis.Results The london planetree P450 genes were divided into 8 clans, 38 families, and 50 subfamilies. Among them, 48 genes showed a strong negative selec-tion of P450 genes between london planetree and Arabidopsis thaliana. Nine P450 genes were sig-nificantly up-regulated at 24 h and 48 h after feeding induction by sycamore lace bug Corythucha ciliata. After Weighted correlation network analysis (WGCNA), the P450 genes were identified by a co-expression network mod-ule after feeding, and we identified CYP74A187 (T107931_c2_g1), CYP94A140 (T107552_c0_g3), and CYP79A208 (T94418_c0_g1) as the three hub genes for the expression module. An additional nine key P450 genes were verified by qPCR. The jasmonic acid (JA) pathway key synthetase gene AOS (CYP74A187) was cloned, homology modeled, and subjected to molecular docking analysis. Conclusion This study identified the P450 gene family members of london planetree, and its expression, evolution, and anti-insect defense response. These data increased our understanding of the bio-logical stress of london planetree.
Uncovering the biosynthetic pathway of bioactive compounds from Eclipta prostrata (L.) L. hairy roots
Authors: Lopes Adriana Aparecida , Souza Giuliana R. S. , França Suzelei de C. , Lourenço Miriam V. 
Published: April 2022

Abstract
Eclipta prostrata (L.) L. is a medicinal plant of the Asteraceae family, and several extracts and isolated compounds of Eclipta prostrata (L.) L. showed a wide range of biological activities such as antimicrobial, anticancer, hepatoprotective, neuroprotective, hair growth promoting activities, and more recently against covid-19. Eclipta prostrata (L.) L. hairy roots produce wedelolactone (WL), demethylwedelolactone (DWL) and 3,5-di-O-caffeoylquinic acid (3,5-diCQA), and there is no data in literature regarding biosynthetic pathways are involved. To verify the biosynthetic route, feeding experiments were carried out using sodium [2-13C]acetate, [3-13C]DL-phenylalanine, and 13C-labeled compounds (WL, DWL and 3,5-diCQA) were detected by ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-QTOF-MS). Analysis showed that the metabolic pathways operative of coumestans (WL and DWL) are derived from acetate and shikimate pathways, while that the phenylpropanoid (3,5-CQA) biosynthesis is exclusively from shikimate pathway.
Pervasive Intronic Polyadenylation Serves as a Potential Source of Cancer Neoantigens
Authors: Ren Xi , Zhang Bin , Li Jia , Manoharan Thamizhanban , Liu Beijia , Song Yangyang , Tian Shuye , Tan Kar-Tong , Ding Lingwen , Li Ying , An Omer , Li Ming , Wu Chan-Shuo , Liu Yang , Teo Boon Heng Dennis , Tang Sze Jing , Lu Jinhua , Hu Yuhui , Chen Wei , Chen Leilei , Chia Gloryn , Yang Henry 
Published: April 2022

Abstract

Tumor-specific neoantigens have emerged as a promising source for cancer immunotherapy. These tumor-specific neoantigens could arise from somatic mutations, aberrant splicing and RNA editing. Since intronic polyadenylation has similar potential as mutations to generate tumor-specific transcripts and peptides, it may serve as another neoantigen source, which has not been explored. We developed a novel computational pipeline for identification of tumor-specific transcripts and their translated neoantigens derived from intronic polyadenylation. Applying it to RNA-seq data from 5,654 tumor samples of various cancers and 11,000 + normal samples of different tissues, we observed widespread tumor-specific intronic polyadenylated transcripts and their corresponding neoantigens. In addition, we also discovered complementary effects of neoantigens derived from different sources, identified neoantigens arising from recurrent intronic polyadenylated transcripts, and validated their immunogenicity. Here, we have demonstrated that we were able to identify and predict neoantigens from intronic polyadenylated transcripts using RNA sequencing data, hence, allowing us to explore such neoantigens as potential candidates for cancer immunotherapy.
Two new umbravirus-like associated RNAs (ulaRNAs) discovered in maize and Johnsongrass from Ecuador
Authors: quito-avila diego fernando , Reyes-Proaño Edison , Mendoza Alma , Margaria Paolo , Menzel Wulf , Bera Sayanta , Simmon Anne E 
Published: April 2022

Abstract
Two new umbravirus-like associated RNAs (ulaRNAs) were found, respectively, in maize and Johnsongrass samples from Ecuador. The complete sequences consist of 3,053 and 3,025 nucleotides, respectively, organized in four open reading frames (ORFs). Genome comparisons showed 58% identity between each other, whereas identities to the closest related viruses ranged from 28 to 60%. Phylogenetic inferences using the full genome, or the amino acid sequence of the RNA-dependent-RNA-polymerase (RdRp) placed both sequences in a clade sharing a most recent ancestor with ulaRNAs from sugarcane and maize, suggesting a monophyletic grass-infecting lineage. Terminal regions exhibit features common to umbraviruses and ulaRNAs.
Mesoscopic mapping of the visual pathway in a female 5XFAD mouse model of Alzheimer's disease
Authors: Nam Yunkwon , Kim Sujin , Kim Jieun , Hoe Hyang-Sook , Moon Minho 
Published: April 2022

Abstract
Synaptic and network disruption is correlated with cognitive decline and loss of sensation in Alzheimer's disease (AD). Surprisingly, amyloid-β (Aβ) deposition and Aβ-induced neurodegeneration appear in the retina at the early-stage of AD. Although these Aβ-related changes in the retina could cause damage to the visual functions, no studies have yet revealed the alteration in the visual pathway of AD. Therefore, we investigated the alteration of visual circuits in the AD mouse model using anterograde neurotracer. We investigated the Aβ accumulation by immunofluorescent staining in the retina and retinorecipient areas of 4- and 12-month-old female 5XFAD transgenic mice. Following intravitreal injection of cholera toxin β subunits (CTβ) tracer, we evaluated the fluorescence intensity of accumulated CTβ in the retinorecipient areas in 4- and 12-month-old female wild-type and 5XFAD mice. Our results demonstrated that Aβ accumulation occurred in the retina and retinorecipient regions of early and late stages of the 5XFAD mice. Retinal efferents to the suprachiasmatic nucleus and lateral geniculate nucleus were impaired in the early-stage of AD. Moreover, retinal connections to the dorsal lateral geniculate nucleus and superior colliculus were degenerated in the late-stage of AD. Although these results provide the first neuroanatomical evidence for decreased visual connectivity in an AD mouse model, it is difficult to explain cell type-specific connectivity. These findings reveal the Aβ-induced visual circuit disturbances at the mesoscale level in both the early and late stages of AD and provide anatomical and functional insights into the visual circuitry of AD.
Management of primary spontaneous pneumothorax in a third-level pediatric surgical center.
Authors: Spezzotto Giovanna , Boscarelli Alessandro , Giangreco Manuela , Ceschiutti Giulia , Codrich Daniela , Scarpa Maria-Grazia , Iaquinto Marianna , Olenik Damiana , Guida Edoardo , Schleef Jurgen 
Published: April 2022

Abstract
INTRODUCTIONThe management of primary spontaneous pneumothorax (PSP) in pediatrics remains controversial. Due to a lack of standardized guidelines, it is based on evidence from adult patients. The aim of this study was to investigate difference in the length of stay (LOS) between various treatment options, the risk of recurrence after non-surgical treatment versus surgery, and the role of computed tomography (CT) in the management of PSP. METHODS We retrospectively reviewed patients admitted to our Pediatric Surgery Unit for an episode of PSP between June 2009 and July 2020. Medical records including clinical presentation at admission, diagnostics, treatments, complications, and LOS were collected. RESULTS Twenty-three patients (22 males and 1 female) were included in this study. Median age was 15.65 (range 9-18). Chest X-rays were performed in all patients and showed 5 small (22%) and 18 large (78%) PSP. Eleven patients (48%) were managed non-operatively with observation alone and a recurrence rate of 18%, chest drain was used in 11 patients with a recurrence rate of 36%, and surgery was deemed necessary as a first treatment choice in one case. Six patients (27%) had an episode of relapse after non-operative management or chest drain placement. Following surgery, a relapse occurred in 2 of the 6 patients.CONCLUSIONSAccording to our experience, small PSP or clinically stable larger PSP can be treated conservatively with observation alone. Operative management should be taken into consideration in children with large symptomatic PSP, persistent air leak, and/or relapse after chest drain insertion. Prospective studies with a greater number of patients are encouraged to establish evidence-based guidelines in the pediatric population.
Meta analysis of the diagnostic efficacy of long non-coding RNA in ischemic stroke
Authors: Zheng* Jiqing , Long* Yaobin , Liu Yun 
Published: April 2022

Abstract
Objective To evaluate the diagnostic efficacy of long non-coding RNA (lncRNA) for acute ischemic stroke (IS) by Meta analysis. Methods This research had obtained a registration number in PROSPERO (CRD 42021283867). The articles of lncRNA diagnosis of IS in PubMed, Web of Science, Cochrane, CBM, China National Knowledge Network, VIP and Wanfang were searched systematically. The papers was evaluated using the QUADAS-2 scale and a bias risk chart – RevMan5.3. Then, threshold effect, non-threshold effect, combined diagnostic efficacy and subgroup analysis of the articles were analyzed by Meta-DISC 1.4. Finally, the summary receiver operating characteristic (SROC) curve was drawn, sensitivity and publication bias were analyzed by stata16. Results This paper covers eleven studies, including 1717 patients with IS and 1652 healthy subjects. The combined sensitivity of lncRNA diagnosis of IS was 0.70 (95%: 0.69～0.72), specificity was 0.71 (CI95%: 0.69～0.72), the positive likelihood ratio was 2.84 (CI95%: 2.25～3.59), the negative likelihood ratio was 0.39 (CI95%: 0.33～0.46), the diagnostic odds ratio was 7.69 (5.2～11.37) and the area under the curve (AUC) was 80%. Subgroup analysis found that among tissue sources, serum samples had the highest comprehensive diagnostic efficiency, up to 88.5%, and the comprehensive diagnostic efficiency within 24 hours was higher than that within 24 to 72 hours, up to 82.6%. In addition, low expression was slightly higher than high expression, reaching 79.8%. Conclusion There is a high diagnosing efficiency shown in IncRNA, which could be expected to become a predictive tool for IS in the future
Sensitivity analysis of geometric error for a novel slide grinder based on improved Sobol method and its application
Authors: Wang Dong , Han Jihui , Wang Liping , Ma Fengju , Ge Ziyang , Li Xuekun 
Published: April 2022

Abstract
In order to improve both the accuracy and efficiency of grinding slide, this paper designs a novel grinder with dual-lead-dual-head. Since the geometric error is one of the major contributors causing machine inaccuracies, the sensitivity analysis is performed to identify the critical geometric error terms, and an application to accuracy self-test is also given. The volumetric model of the grinder with 38 geometric errors is built by using the homogeneous transformation matrix (HTM) and multi-body system (MBS) theory. An improved Sobol method with quasi-Monte-Carlo algorithm is utilized to perform the global sensitivity analysis (GSA) in the entire workspace. The particular sensitivity analysis is further carried out on the basis of the machining characteristics of a typical slide. All sensitivity analysis results are validated through the error compensation simulations. Besides that, some discussions are given to determine the total critical errors of the grinder considering both entire workspace and particular machining requirements. Finally, the pitch and yaw errors between the dual-grinding-head are investigated, and based on the sensitivity analysis results, a quick accuracy self-test approach is proposed to reduce the measurement load in practice.
Spatio-Temporal Modelling of Childhood Vaccine Uptake in Nigeria: A Hierarchical Bayesian Zero-Inflated Poisson Approach
Authors: Lawal Temitayo Victor , Atoloye Kehinde Adebola , Adebowale Ayo Stephen , Fagbamigbe Adeniyi Francis 
Published: April 2022

Abstract
BACKGROUND
Globally, child mortality and morbidity remain a serious health challenge and infectious diseases are the leading causes. The use of count models together with spatial analysis of the number of vaccines taken is limited in the literature. We used Spatio-temporal in a Bayesian zero-inflated Poisson regression model to assess the number of vaccine uptake among children aged 12–23 months and their associated factors.
METHODS
Data of 19,564 children from 2003, 2008, 2013 and 2018 population-based cross-sectional Nigeria Demographic and Health Survey were used. The childhood vaccines include one dose of Bacillus-Calmette-Guérin; three doses of Diphtheria-Pertussis-Tetanus; three doses of Polio and one dose of Measles. Uptake of all nine vaccines was regarded as full vaccination. We examined the multilevel factors associated with the number of vaccine uptake using descriptive, bivariable and multivariable Bayesian models. Analysis was conducted in Stata version 16 and R statistical packages, and visualization in ArcGIS.
RESULTS
Prevalence of full vaccination was 6.5% in 2003, 14.8% in 2008, 21.8% in 2013 and 23.3% in 2018. Full vaccination coverage ranged from 1.7% in Sokoto to 51.9% in Anambra. Factors associated with the number of vaccines include maternal age (adjusted Incidence “risk” Ratio (aIRR) = 1.05; 95% Credible Interval (CrI) = 1.03–1.07) for 25–34 years and (aIRR = 1.07; 95% CrI = 1.05–1.10) for 35–49 years and education: (aIRR = 1.11, 95% CrI = 1.09–1.14) for primary and (aIRR = 1.16; 95% CrI = 1.13–1.19) for secondary/tertiary education. Other significant factors are wealth status, antenatal care attendance, working status, use of skilled birth attendant, religion, mother’s desire for the child, community poverty rate, community illiteracy, and community unemployment.
CONCLUSION
Although full vaccination has remained low, there have been improvements over the years with wide disparities across the states. Improving uptake of vaccines by educating women on the benefits of hospital delivery and vaccines through radio jingles and posters should be embraced, and state-specific efforts should be made to address inequality in access to routine vaccination in Nigeria.
In-process identification of milling parameters based on digital twin driven intelligent algorithm
Authors: Zheng Charles Ming , Zhang Lu , Kang Yaw-Hong , Zhan Youji , Xu Yongchao 
Published: April 2022

Abstract
The potential benefits of Industry 4.0 have led to an increased interest in smart manufacturing. To facilitate the self-diagnosis and adaptive ability in smart milling system, a digital twin driven intelligent algorithm for monitoring in-process milling parameters is proposed here. The algorithm can extract the radial width of cut, axial depth of cut, cutter runout parameters and cutting constants in the end milling process at the same time only by using force sensor. It is an important breakthrough in this paper to converge two different force models to realize cyber-physical fusion for identifying milling parameters in the milling process. By using the convolution force model, digital twin technology can extract the approximate solution of milling parameters in the machining process in advance, so as to narrow the range of solution. Furthermore, the subsequent artificial intelligence algorithm can find the accurate solution of the current milling parameters in a short calculation time by cyber-physical fusion with the numerical force model considering cutter runout effect. Milling experiments are carried out to validate the proposed algorithm. It is shown that due to the complementary advantages of the convolution force model and numerical force model, the algorithm proposed in this paper can give consideration to the identification accuracy and calculation efficiency.