bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bifidobacterium breve MCC1274 Supplementation Increased the Plasma Levels of Metabolites with Potential Anti-Oxidative Activity in APP Knock-In Mice
Authors: Ohno Kazuya , Abdelhamid Mona , Zhou Chunyu , Jung Cha-Gyun , Michikawa Makoto 
Published: August 2022

Background: We previously reported the effects of a probiotic strain, Bifidobacterium breve MCC1274, in improving cognitive function in preclinical and clinical studies. Recently, we demonstrated that supplementation of this strain led to decreased amyloid-β production, attenuated microglial activation, and suppressed inflammation reaction in the brain of APP knock-in (AppNL - G - F) mice. Objective: In this study, we investigated the plasma metabolites to reveal the mechanism of action of this probiotic strain in this Alzheimer’s disease (AD)-like model. Methods: Three-month-old mice were orally supplemented with B. breve MCC1274 or saline for four months and their plasma metabolites were comprehensively analyzed using CE-FTMS and LC-TOFMS. Results: Principal component analysis showed a significant difference in the plasma metabolites between the probiotic and control groups (PERMANOVA, p = 0.03). The levels of soy isoflavones (e.g., genistein) and indole derivatives of tryptophan (e.g., 5-methoxyindoleacetic acid), metabolites with potent anti-oxidative activities were significantly increased in the probiotic group. Moreover, there were increased levels of glutathione-related metabolites (e.g., glutathione (GSSG)_divalent, ophthalmic acid) and TCA cycle-related metabolites (e.g., 2-Oxoglutaric acid, succinic acid levels) in the probiotic group. Similar alternations were observed in the wild-type mice by the probiotic supplementation. Conclusion: These results suggest that the supplementation of B. breve MCC1274 enhanced the bioavailability of potential anti-oxidative metabolites from the gut and addressed critical gaps in our understanding of the gut-brain axis underlying the mechanisms of the probiotic action of this strain in the improvement of cognitive function.
The denouement of ozone therapy vying to chlorhexidine in non surgical periodontal therapy
Authors: Pendyala Gowri , Joshi Saurabh , Mani Ameet , Dhole Sudhir , Kale Preeti 
Published: August 2022

BACKGROUND: Scaling and root planing [SRP] being the mainstay of treatment of periodontitis encompasses unambiguous impediments. antiseptics represent an aid to nonsurgical periodontal therapy. OBJECTIVE: This randomized controlled, split mouth study design with an observation period of three months aims to clinically evaluate the efficacy of ozonised oil and chlorhexidine as an adjunct to SRP. METHODS: Twenty-five patients of both sexes with an age range of 30–65 years diagnosed with chronic periodontitis and having a periodontal probe depth (PD)≥5 mm and CAL≥3 mm on at least 1 site in each quadrant were included in this randomised split mouth design study. Patients were allocated in 2 experimental treatment groups as SRP + chlorhexidine gel (control sites) and with SRP + ozone oil (test sites). The plaque index (PI), gingival index (GI), and periodontal pocket depth (PPD), clinical attachment level (CAL) were recorded at baseline data and after 30 days post-baseline. RESULTS: The present study showed significant results in both the groups with regards to the improvement in the clinical parameters. When comparison was made between the two groups, it has been assessed that the use of the ozonized oil in addition to SRP did not show significant differences when compared to conventional SRP + chlorhexidine. CONCLUSION: For bye to SRP, ozonized oil can be considered as a viable alternative to chlorhexidine in the treatment of periodontitis, especially considering its low toxicity compared to chlorhexidine.
Pengaruh Praktikum Perubahan Wujud Materi Berbasis Video Terhadap Pemahaman Konsep Siswa Kelas V Sekolah Dasar
Authors: Baitty Aulia Nur , Sukmawati Wati 
Published: August 2022

Video based practicum learning is very important to further develop and understanding of science concepts for elementary school students. This researcher aims to determine the effect of the video based material transformation practicum on the understanding of the concepts of 5th graders students at SDN Duren Sawit 07 Jakarta. The research used a quasi-experimental quantitative method with a Nonequivalent Control Group Design. The place and population of the research, were the 5th grade students of SDN Duren Sawit 07 Jakarta, totaling 56 students, divided into experimental class and control class. Information was collected using pretest and posttest question. This show that the typical value of the experimental class and control class using the N-Gain equation is 70,72% in the experimental class and 43,32% in the control class. The strategy of testing hypothesis information using Peireds T-Test obtained an importance value of 0,000 < 0,05. The results showed that there was an effect that video based practicum could increase understanding of science concepts for 5th grade elementary school students.
Pembelajaran praktikum berbasis video sangat penting untuk lebih mengembangkan teoritis dan pemahaman konsep sains peserta didik sekolah dasar. Penelitian ini bertujuan untuk mengetahui pengaruh praktikum perubahan wujud materi berbasis video terhadap pemahaman konsep siswa kelas V SDN Duren Sawit 07 Jakarta. Penelitian tersebut menggunakan metode kuantitatif quasi experimental dengan pendekatan Nonequivalent Control Group Design. Tempat dan jumlah populasi penelitian adalah peserta didik kelas V SDN Duren Sawit 07 Jakarta yang berjumlah 56 peserta didik, dibagi menjadi kelas eksperimen dan kelas kontrol. Informasi dikumpulkan dengan menggunakan pretest dan posttest. Menunjukkan bahwa nilai mean pemahaman konsep IPA kelas eksperimen dan kelas kontrol yang menggunakan persamaan N-Gain adalah 70,72% di kelas eksperimen dan 43,32% di kelas kontrol. Strategi pengujian informasi hipotesis dengan menggunakan uji-t Peired diperoleh nilai sig 0,000 < 0,05. Hasil penelitian menunjukkan bahwa terdapat pengaruh praktikum berbasis video dapat meningkatkan pemahaman konsep IPA bagi peserta didik kelas V SD.
Islamic Education and Socio-Cultural Development in Educational Institutions
Authors: Mansir Firman 
Published: August 2022

Religious and socio-cultural education is inseparable in peoples lives. Educational cultural proximity to religion and socio-cultural development become two interrelated and mutually needy sides in solving social problems of society. This research shows that there is a relationship between religious and cultural education that is interrelated, giving birth to changes and responding to the rapid development of the times, thus ushering in a reality of religious life that is full of educational values without losing the local culture. The success of a nation can be seen and measured by the younger generation of its nation in the present and the future. Regarding religious education with culture, it is hoped that there will be the best results from a new generation and have potential with good quality, who can develop the knowledge they have and apply it well in the fabric of education, society, and culture. Thus, religious and socio-cultural education provides answers to various problems in the social development of budaya to religion in the context of educational institutions, be it in schools or madrasas.
Pendidikan Agama Islam dan sosial budaya tidak dapat dipisahkan dalam kehidupan masyarakat. Pendekatan kultural edukatif terhadap agama dan perkembangan sosial budaya menjadi dua sisi yang saling terkait dan saling membutuhkan dalam memecahkan persoalan sosial masyarakat. Penelitian ini menunjukkan bahwa terdapat hubungan antara pendidikan Agama Islam dan budaya yang saling berkaitan, dengan melahirkan perubahan serta merespon berkembangnya zaman yang semakin pesat, sehingga mengantarkan pada sebuah kenyataan kehidupan beragama yang sarat dengan nilai-nilai pendidikan tanpa menghilangkan budaya setempat. Berhasilnya suatu bangsa dapat dilihat serta diukur dari generasi muda bangsanya pada masa kini serta pada masa yang akan datang. Dalam hubungannya Pendidikan Agama Islam dengan budaya, sangat diharapkan adanya hasil terbaik dari generasi yang baru dan memiliki potensi dengan kualitas yang baik, yang mampu untuk mengembangkan ilmu pengetahuan yang dimiliki dan mengaplikasikannya dengan baik dalam jalinan pendidikan, sosial dan budaya. Dengan demikian, Pendidikan Agama Islam dan sosial budaya memberi jawaban dari berbagai permasalahan dalam perkembangan sosial budaya terhadap agama dalam konteks lembaga pendidikan, baik itu di sekolah maupun madrasah.
Strategi Peningkatan Kualitas Pelayanan Publik Pegawai Kantor Kecamatan Malinau Barat Kabupaten Malinau
Authors: Malohing Mince Caroline , Milwan Milwan , Wibowo Soesilo 
Published: August 2022

The purpose of this research is to study & analysis increasing quality strategy for public service & do effort for increasing quality strategy for public service. This research is done by qualitative approaching so that the researcher will be able to observe carefully & deeply through direct approach. The researcher uses interviewing and observating data not only reports but also documents which are related with the research. Research analysis results show that from tangible dimension overall has not shown good results since some indicators show negative results, reliability, responsiveness, and assurance dimension shows good results, namun dimension emphaty still shows negative due to aspect that needs to be improved. It is hospitality in doing public service for public. Internal strategy through SWOT analysis placed district office West Malinau on the third quadrant so the strategy that can be used is by changing the strategy to take advantage of opportunities and minimize the weaknesses.

Tujuan penelitian ini untuk menganalisis kualitas layanan publik pegawai dan strategi internal di Kantor Kecamatan Malinau Barat, Kabupaten Malinau. Pendekatan kualitatif dan kuantitatif dipadukan supaya dapat mengobservasi secara detail, mendalam, dan rinci terhadap objek yang diamati. Teknik pengumpulan data dengan metode wawancara, observasi, serta mempelajari data berupa laporan dan dokumen yang berkaitan. Hasil analisis penelitian menunjukkan kualitas pegawai ditinjau dari dimensi tangible secara keseluruhan belum menunjukkan hasil yang positif karena beberapa indikator menunjukkan hasil negatif, sedangkan ditinjau dari dimensi reliability, responsiveness dan assurance menunjukkan hasil yang positif, namun ditinjau dari dimensi emphaty belum menunjukkan hasil positif, karena terdapat aspek yang harus dilakukan perbaikan, yaitu keramahan pegawai dalam memberikan pelayanan kepada masyarakat. Strategi internal melalui analisis SWOT menempatkan Kantor Kecamatan Malinau Barat pada kuadran ke tiga, sehingga strategi yang dapat dilakukan adalah melakukan perubahan strategis dengan memanfaatkan peluang yang dimiliki untuk meminimalisir kelemahan yang dimiliki.
Two-dimensional ultrathin Ti3C2 MXene nanosheets coated intraocular lens for synergistic photothermal and NIR-controllable rapamycin releasing therapy against posterior capsule opacification
Authors: Ye Zi , Huang Yang , Li Jinglan , Ma Tianju , Gao Lixiong , Hu Huihui , He Qing , Jin Haiying , Li Zhaohui 
Published: August 2022

Posterior capsule opacification (PCO) is one of the most frequent late-onset complications after cataract surgery. Several kinds of drug-eluting intraocular lenses (IOL) were designed for sustainable drug release to suppress ocular inflammation, the proliferation of lens epithelial cells (LECs) and the development of PCO after cataract surgery. Despite previous advances in this field, the drug-loaded IOLs were limited in ocular toxicity, insufficient drug-loading capacity, and short release time. To prevent PCO and to address these drawbacks, a novel drug-loaded IOL (Rapa@Ti3C2-IOL), prepared from two-dimensional ultrathin Ti3C2 MXene nanosheets and rapamycin (Rapa), was fabricated with a two-step spin coating method in this study. Rapa@Ti3C2 was prepared via electrostatic self-assembly of Ti3C2 and Rapa, with a loading capacity of Rapa at 92%. Ti3C2 was used as a drug delivery reservoir of Rapa. Rapa@Ti3C2-IOL was designed to have the synergistic photothermal and near infrared (NIR)-controllable drug release property. As a result, Rapa@Ti3C2-IOL exhibited the advantages of simple preparation, high light transmittance, excellent photothermal conversion capacity, and NIR-controllable drug release behavior. The Rapa@Ti3C2 coating effectively eliminated the LECs around Rapa@Ti3C2-IOL under a mild 808-nm NIR laser irradiation (1.0 W/cm−2). Moreover, NIR-controllable Rapa release inhibited the migration of LECs and suppressed the inflammatory response after photothermal therapy in vitro. Then, Rapa@Ti3C2-IOL was implanted into chinchilla rabbit eyes, and the effectiveness and biocompatibility to prevent PCO were evaluated for 4 weeks. The Rapa@Ti3C2-IOL implant exhibited excellent PCO prevention ability with the assistance of NIR irradiation and no obvious pathological damage was observed in surrounding healthy tissues. In summary, the present study offers a promising strategy for preventing PCO via ultrathin Ti3C2 MXene nanosheet-based IOLs with synergistic photothermal and NIR-controllable Rapa release properties.
Towards automatic reconstruction of 3D city models tailored for urban flow simulations
Authors: Pađen Ivan , García-Sánchez Clara , Ledoux Hugo 
Published: August 2022

In the computational fluid dynamics simulation workflow, the geometry preparation step is often regarded as a tedious, time-consuming task. Many practitioners consider it one of the main bottlenecks in the simulation process. The more complex the geometry, the longer the necessary work, meaning this issue is amplified for urban flow simulations that cover large areas with complex building geometries. To address the issue of geometry preparation, we propose a workflow for automatically reconstructing simulation-ready 3D city models. The workflow combines 2D geographical datasets (e.g., cadastral data, topographic datasets) and aerial point cloud-based elevation data to reconstruct terrain, buildings, and imprint surface layers like water, low vegetation, and roads. Imprinted surface layers serve as different roughness surfaces for modeling the atmospheric boundary layer. Furthermore, the workflow is capable of automatically defining the influence region and domain size according to best practice guidelines. The resulting geometry aims to be error-free: without gaps, self-intersections, and non-manifold edges. The workflow was implemented into an open-source framework using modern, robust, and state-of-the-art libraries with the intent to be used for further developments. Our approach limits the geometry generation step to the order of hours (including input data retrieval and preparation), producing geometries that can be directly used for computational grid generation without additional preparation. The reconstruction done by the algorithm can last from a few seconds to a few minutes, depending on the size of the input data. We obtained and prepared the input data for our verification study in about 2 hours, while the reconstruction process lasted 1 minute. The unstructured computational meshes we created in an automatic mesh generator show satisfactory quality indicators and the subsequent numerical simulation exhibits good convergence behavior with the grid convergence index of observed variables less than 5%.
The clinical impact of serum 1,5-anhydro-D-glucitol levels on coronary artery calcification and adverse outcomes assessed by coronary optical coherence tomography in diabetic patients
Authors: Teng Hsin-I , Chen Hsiang-Yao , Tsai Chuan-Tsai , Huang Wei-Chieh , Chen Ying-Ying , Hsueh Chien-Hung , Hau William K. , Lu Tse-Min 
Published: August 2022

BackgroundSerum 1,5-anhydro-D-glucitol (1,5-AG) is a novel biomarker for short-term glycemic status and postprandial hyperglycemia. The association between serum 1,5-AG levels and coronary artery calcification (CAC) through a quantitative assessment using optical coherence tomography (OCT) is unclear. We aimed to evaluate this association using OCT in patients with diabetes mellitus (DM).MethodsFrom June 2016 to December 2019, we prospectively enrolled 256 patients who underwent OCT-guided percutaneous coronary intervention (PCI). Half of the patients had diabetes. Patients were followed up for a mean period of 1.8 ± 0.8 years (median: 2.2 years). The relative calcium index and relative lipid core index measured by quantitative OCT analysis were used to evaluate the intra-plaque calcium and lipid levels of culprit plaques. We also analyzed the correlation between serum 1,5-AG levels and long-term major adverse cardiovascular events.ResultsSerum 1,5-AG levels were significantly lower in diabetic patients than in non-diabetic patients (DM vs. non-DM: 55.6 ± 27.9 μg/mL vs. 63.7 ± 26.1 μg/mL, p = 0.016), and lower in fibrocalcified lesions than in fibrotic or fibrolipidic lesions (fibrocalcified vs. fibrotic or fibrolipidic: 42.8 ± 19.1 vs. 72.9 ± 25.2 or 66.4 ± 27.5 μg/mL, p &lt; 0.001, respectively). In addition, we found a significant inverse correlation between serum 1,5-AG levels and relative calcium index (r = −0.729, p &lt; 0.001). In multivariate Cox regression analysis, low serum 1,5-AG level was identified as an independent predictor for major adverse cardiovascular events in diabetic patients (p = 0.043), but not in non-diabetic patients (p = 0.748) after adjusting for age and sex.ConclusionThis study revealed that low serum 1,5-AG levels were associated with an increased risk of CAC as assessed by OCT, especially in diabetic patients. Low serum 1,5-AG levels may predict future major adverse cardiovascular events in diabetic patients undergoing OCT-guided PCI.
Mindful practice for teachers: Relieving stress and enhancing positive mindsets
Authors: Carroll Annemaree , Hepburn Stevie-Jae , Bower Julie 
Published: August 2022

ObjectivesThe present study aimed to determine the degree of stress relief and increase in mindfulness attributes experienced by 18 participants in an 8-week Mindful Practice for Teachers (MPT) program.MethodsThe mixed-methods study design included participant reflections collected throughout the study and self-report measures: Perceived Stress Scale (PSS), Positive Mindset Index (PMI), and Five Facets of Mindfulness Questionnaire (FFMQ).Data analysisPre- and post-program paired-samples t-tests were completed for the self-report measures. Inductive coding was completed for the participant reflections.ResultsThe participants (N = 18) reported a significant decrease in perceived stress pre-program and post-program. The significant changes in the sub-scales of the FFMQ were reported for observing, non-reaction, and non-judgment pre-program and post-program. A statistically significant change was reported for the PMI sub-scale stability which increased post-program. According to participant reflections, there was an increased awareness of the physiological response during positive and challenging events/incidents. The participants reported positive satisfaction pertaining to program materials, exercises, and activities.ConclusionFindings indicated that the participants experienced a decrease in their perceived level of stress and an increase in their mindfulness attributes consequently highlighting the benefits of mindfulness-based programs for teacher stress management. The MPT program may prove to be a positive intervention for managing work-related stress and providing teachers with simple, stress management resources.
Systematic identification of aberrant non-coding RNAs and their mediated modules in rotator cuff tears
Authors: Zhang Yichong , Chen Jianhai , He Shengyuan , Xiao Yun , Liu Aiyu , Zhang Dianying , Li Xia 
Published: August 2022

Background: Rotator cuff tears (RCT) is the most common cause of shoulder dysfunction, however, its molecular mechanisms remain unclear. Non-coding RNAs(ncRNAs), such as long ncRNA (lncRNA), microRNA (miRNA) and circular RNA (circRNA), are involved in a variety of diseases, but little is known about their roles in RCT. Therefore, the purpose of this study is to identify dysregulated ncRNAs and understand how they influence RCT.Methods: We performed RNA sequencing and miRNA sequencing on five pairs of torn supraspinatus muscles and matched unharmed subscapularis muscles to identify RNAs dysregulated in RCT patients. To better comprehend the fundamental biological processes, we carried out enrichment analysis of these dysregulated mRNAs or the co-expressed genes of dysregulated ncRNAs. According to the competing endogenous RNA (ceRNA) theory, we finally established ceRNA networks to explore the relationship among dysregulated RNAs in RCT.Results: A total of 151 mRNAs, 38 miRNAs, 20 lncRNAs and 90 circRNAs were differentially expressed between torn supraspinatus muscles and matched unharmed subscapularis muscles, respectively. We found that these dysregulated mRNAs, the target mRNAs of these dysregulated miRNAs or the co-expressed mRNAs of these dysregulated ncRNAs were enriched in muscle structure development, actin-mediated cell contraction and actin binding. Then we constructed and analyzed the ceRNA network and found that the largest module in the ceRNA network was associated with vasculature development. Based on the topological properties of the largest module, we identified several important ncRNAs including hsa_circ_0000722, hsa-miR-129-5p and hsa-miR-30c-5p, whose interacting mRNAs related to muscle diseases, fat and inflammation.Conclusion: This study presented a systematic dissection of the expression profile of mRNAs and ncRNAs in RCT patients and revealed some important ncRNAs which may contribute to the development of RCT. Such results could provide new insights for further research on RCT.
A novel method to purify small RNAs from human tissues for methylation analysis by LC-MS/MS
Authors: Yang Rong , Li Jianfeng , Wu Yifan , Jiang Xinli , Qu Shuang , Wang Qiang , Liang Hongwei , Zen Ke 
Published: August 2022

Methylation modification of small RNAs, including miRNA, piRNA, and tsRNA, is critical for small RNA biogenesis and biological function. Methylation of individual small RNA can be defined by liquid chromatography-coupled with mass spectrometry (LC-MS/MS). However, LC-MS/MS analysis requires a high purity of individual small RNA. Due to the difficulty of purifying specific small RNA from tissues or cells, the progress in characterizing small RNA methylation by LC-MS/MS is limited. Here, we report a novel method that can efficiently purify small RNA from human tissues for LC-MS/MS analysis. This method includes two steps: 1) pull down the target small RNA by incubating total small RNAs (18–24 nt) extracted from human tissues with a biotinylated antisense oligonucleotide of the target small RNA, followed by capturing the binding duplex of biotinylated antisense and small RNA via streptavidin magnetic beads, and 2) protect the target small RNA by pairing it with a single-strand DNA, which sequence is complementary to the target small RNA, to form a DNA/RNA hybrid double-strand, followed by sequential digestion with exonuclease I, nuclease S1, and DNase I, respectively. Furthermore, employing a mixture of four pairs of synthetic methylated and non-methylated small RNAs, we further refined this two-step method by optimizing the nuclease S1 treatment condition. With this method, we successfully purified miR-21-5p, miR-26-5p, piR-020485, and tsRNA from human lung and sperm tissue samples and analyzed their 2′-O-methylation modification at the 3′-end by LC-MS/MS.