bluuebunny/crossref_metadata_2025_split huggingface dataset:
Living with parents, lifestyle pattern and common mental disorders in adolescents: a school-based study in Brazil
Authors: Gratão Lucia Helena Almeida , Pessoa Milene Cristine , Rocha Luana Lara , da Silva Thales Philipe Rodrigues , Santos Freitas Eloar dos , de Oliveira Tatiana Resende Prado Rangel , de Freitas Cunha Cristiane , Mendes Larissa Loures 
Published: October 2022

Abstract
Background
Mental health conditions represent 16% of the global burden of disease and injury in adolescents. Promotion, protection, and restoring the mental health must be considered indispensable, especially in adolescence. This study aims to verify the association of lifestyle pattern, living with parents and the presence of Common Mental Disorders (CMD) in Brazilian adolescents.

Methods
Cross-sectional study that analyzed data from 71,553 adolescents aged 12–17 years, from the Study on Cardiovascular Risks in Adolescents (ERICA), between 2013 and 2014. Principal Component Analysis (PCA) was performed to identify lifestyle pattern, and Logistic Regression Models were performed to identify the associations between lifestyle pattern, living with parents, and presence of CMD.

Results
To construct the Common Mental Disorders (CMD) variable, the Goldberg General Health Questionnaire was used. The Pattern of Healthy Lifestyle Practices found was characterized by higher water consumption, lower consumption of ultra-processed foods, the habit of eating breakfast, less exposure time to screens, habit of physical activity, and longer mean sleep time in hours. Adolescents belonging to the second (OR: 0.73; 95% CI 0.65–0.82) and third (OR: 0.44; 95% CI 0.39–0.50) terciles of the pattern, that is, those who had higher belonging to the pattern had lower chances of having CMD. Adolescents who lived with neither parent (OR: 1.44; 95% CI 1.16–1.78) were associated with a higher chance to present CMD.

Conclusion
Living with parents can contribute to better mental health among adolescents. In addition, the adoption of a healthy lifestyle, encouraged by parents and the community, can reduce the chances of CMD in Brazilian adolescents.
Attitude and intention of migrant populations in the Netherlands regarding female genital mutilation/cutting
Authors: Kawous Ramin , Allwood Emily , Middelburg Annemarie , van der Kwaak Anke , Geraci Diana , Bos Marthine , van den Muijsenbergh Maria E. T. C. 
Published: October 2022

Abstract
Background
Due to migration, the practice female genital mutilation/cutting (FGM/C) has become an issue of concern in high-resource countries such as the Netherlands. It was therefore of utmost importance to explore the attitude and intention of migrant populations in the Netherlands towards FGM/C, which may be leveraged to promote its elimination. Therefore, the aim of the present study was to explore the attitude and intention of migrant populations in the Netherlands regarding FGM/C.

Methods
A qualitative study design was employed using Theory of Planned Behaviour (TPB) as a framework for the analysis. Data were collected using focus-group discussions (FGDs) and individual interviews. The FGDs and individual interviews were audio-recorded and transcribed verbatim. The main topics of the interviews were based on the constructs of TPB (attitude, subjective norms, perceived behavioral control and intention). Thus, concerning the development of categories, we opted for a hybrid form using a deductive as well as an inductive approach.

Results
A total of 55 participants, 15 men and 40 women (9 born in the Netherlands) participated in the study. The findings showed that as a result of migration and regardless of country of origin and gender, many participants have changed their attitudes towards the abandonment of FGM/C. None of the participants intended to have FGM/C performed on their daughters. Generally, the social pressure to perform FGM/C seems to be lower in the Netherlands when compared to the country of origin. Most participants felt confident in their ability to resist social pressure. However, some participants feared that they might succumb to social pressure or feared that their daughters would undergo FGM/C without their consent.

Conclusion
This study aimed to explore the attitude and intention of migration populations in the Netherlands regarding FGM/C. Our findings showed that the study participants had no intention to perform FGM/C on their daughters. As a consequence of acculturation process, interest in the practice of FGM/C could wane following migration. Nonetheless, some pressure to perform FGM/C still exists after migration. Newly arrived migrants and those more vulnerable to social pressure, may benefit from educational interventions that increases knowledge and awareness about various aspects of the practice, with an emphasis on empowering those individuals in facing social pressure.
Muslim nurse’s spiritual sensitivity as a higher perception and reflection toward spiritual care: a qualitative study in southeast Iran
Authors: Akbari Omolbanin , Dehghan Mahlagha , Tirgari Batool 
Published: October 2022

Abstract
Background
Spiritually sensitive nurses perceive the spiritual attitudes and feelings of others. They play a positive role in providing spiritual care to patients. Spiritually sensitive nurses deal appropriately with suffering, frustration, and spiritual dysfunction. Therefore, the present study aimed to explain Iranian nurses’ experiences of spiritual sensitivity.

Methods
This qualitative descriptive explorative study used conventional content analysis and purposeful sampling to explain the experiences of Iranian nurses (n = 19). This study used in-depth semi-structured interviews with 19 nurses, as well as maximum variation sampling to gather rich information (age, sex, religion, work experience, level of education, marital status, type of hospital and ward) from March 2021 to January 2022. The current study also employed Guba & Lincoln criteria to increase data trustworthiness and Graneheim and Lundman approach to analyze the content.

Results
The research data showed 497 codes, 1 theme, 3 categories, and 6 subcategories. The theme of “Nurse’s spiritual sensitivity as a higher perception and reflection toward spiritual care” included three categories of the spiritual and professional character of the nurse, perception of the spiritual needs of patients and their families, and the nurse’s reflection on the religious beliefs of patients and their families.

Conclusion
Spiritual sensitivity helps a nurse to provide holistic care for patients and their families. Therefore, managers and policymakers should create guidelines to help nurses become more spiritually sensitive as well as to meet spiritual needs of patients. Further quantitative and qualitative research should confirm these results in other social and cultural contexts.
Necrotizing hepatitis caused by Clostridium novyi type B in a dog with no predisposing liver lesions: a case report
Authors: Trusiano Brie , Todd S. Michelle , Barrett Sarah , Ciepluch Michael , Fox Alexandra , McClendon Diamond , Lahmers Kevin K. , Oakes Vanessa J. , Carvallo Francisco , Corrigan Virginia , LeCuyer Tessa E. 
Published: October 2022

AbstractBackgroundInfectious necrotic hepatitis (INH) is typically a disease of ruminants caused byClostridium novyitype B. Growth of the causative agent is supported by development of an anaerobic environment within the liver. In dogs,C. novyiis rare and has only been previously reported as a post-mortem diagnosis. In one case, infection was secondary to metastatic pancreatic adenocarcinoma and the other was presumptively diagnosed on histopathology of a hepatic lesion in a dog initially presented for acute collapse.Case presentationAn 8-year-old spayed, female mixed breed dog was presented for acute onset of hyporexia and vomiting. Serum biochemistry revealed elevated hepatocellular injury and cholestatic liver enzymes. Ultrasound revealed peritoneal fluid accumulation and multiple hepatic masses. Cytologic examination of liver aspirates and peritoneal fluid revealed frequent 4 × 1 μm bacilli with a terminal endospore. Anaerobic bacterial growth isolated from the fluid sample could not be identified using typical laboratory identification techniques. Long-read, whole genome sequencing was performed, and the organism was identified asClostridium novyitype B. Antimicrobial and hepatic support treatment were initiated. The patient re-presented 27 days later, and the follow up liver aspirate with cytology revealed no appreciable bacteria and anaerobic culture was negative. The patient was presented four months later and a large hepatic mass and peritoneal fluid were again identified on abdominal ultrasound. Cytologic examination of the peritoneal fluid revealed bacilli similar to those identified on initial presentation. The patient was euthanized. The most significant finding on necropsy was necrotizing hepatitis with intralesional endospore-forming bacilli compatible with recurrence ofClostridium novyitype B. There was no identifiable cause of an anaerobic insult to the liver.ConclusionsThis case demonstrates the diagnostic utility of using cytology as part of the initial diagnostic work up for infectious hepatitis. The cytologic findings coupled with whole genome sequencing and anaerobic culture were crucial for the identification and classification of the organism identified on fine needle aspirate.Clostridium novyitype B should be considered when bacilli organisms containing a terminal endospore are identified on liver aspirates collected from canine patients.
Stem cell secretome as a mechanism for restoring hair loss due to stress, particularly alopecia areata: narrative review
Authors: Salhab Ola , Khayat Luna , Alaaeddine Nada 
Published: October 2022

Abstract
Background
Living organisms are continuously exposed to multiple internal and external stimuli which may influence their emotional, psychological, and physical behaviors. Stress can modify brain structures, reduces functional memory and results in many diseases such as skin disorders like acne, psoriasis, telogen effluvium, and alopecia areata. In this review, we aim to discuss the effect of secretome on treating alopecia, especially alopecia areata. We will shed the light on the mechanism of action of the secretome in the recovery of hair loss and this by reviewing all reported in vitro and in vivo literature.

Main body
Hair loss has been widely known to be enhanced by stressful events. Alopecia areata is one of the skin disorders which can be highly induced by neurogenic stress especially if the patient has a predisposed genetic background. This condition is an autoimmune disease where stress in this case activates the immune response to attack the body itself leading to hair cycle destruction. The currently available treatments include medicines, laser therapy, phototherapy, and alternative medicine therapies with little or no satisfactory results. Regenerative medicine is a new era in medicine showing promising results in treating many medical conditions including Alopecia. The therapeutic effects of stem cells are due to their paracrine and trophic effects which are due to their secretions (secretome).

Conclusion
Stem cells should be more used as an alternative to conventional therapies due to their positive outcomes. More clinical trials on humans should be done to maximize the dose needed and type of stem cells that must be used to treat alopecia areata.
The economic burden of malaria: a systematic review
Authors: Andrade Mônica V. , Noronha Kenya , Diniz Bernardo P. C. , Guedes Gilvan , Carvalho Lucas R. , Silva Valéria A. , Calazans Júlia A. , Santos André S. , Silva Daniel N. , Castro Marcia C. 
Published: October 2022

Abstract
Background
Quantifying disease costs is critical for policymakers to set priorities, allocate resources, select control and prevention strategies, and evaluate the cost-effectiveness of interventions. Although malaria carries a very large disease burden, the availability of comprehensive and comparable estimates of malaria costs across endemic countries is scarce.

Methods
A literature review to summarize methodologies utilized to estimate malaria treatment costs was conducted to identify gaps in knowledge.

Results
Only 45 publications met the inclusion criteria. They utilize different methods, include distinct cost components, have varied geographical coverage (a country vs a city), include different periods in the analysis, and focus on specific parasite types or population groups (e.g., pregnant women).

Conclusions
Cost estimates currently available are not comparable, hindering broad statements on the costs of malaria, and constraining advocacy efforts towards investment in malaria control and elimination, particularly with the finance and development sectors of the government.
LPAR5 confers radioresistance to cancer cells associated with EMT activation via the ERK/Snail pathway
Authors: Sun Xiao-Ya , Li Hao-Zheng , Xie Da-Fei , Gao Shan-Shan , Huang Xin , Guan Hua , Bai Chen-Jun , Zhou Ping-Kun 
Published: October 2022

Abstract
Background
Epithelial-to-mesenchymal transition (EMT) is a critical event contributing to more aggressive phenotypes in cancer cells. EMT is frequently activated in radiation-targeted cells during the course of radiotherapy, which often endows cancers with acquired radioresistance. However, the upstream molecules driving the signaling pathways of radiation-induced EMT have not been fully delineated.

Methods
In this study, RNA-seq-based transcriptome analysis was performed to identify the early responsive genes of HeLa cells to γ-ray irradiation. EMT-associated genes were knocked down by siRNA technology or overexpressed in HeLa cells and A549 cells, and the resulting changes in phenotypes of EMT and radiosensitivity were assessed using qPCR and Western blotting analyses, migration assays, colony-forming ability and apoptosis of flow cytometer assays.

Results
Through RNA-seq-based transcriptome analysis, we found that LPAR5 is downregulated in the early response of HeLa cells to γ-ray irradiation. Radiation-induced alterations in LPAR5 expression were further revealed to be a bidirectional dynamic process in HeLa and A549 cells, i.e., the early downregulating phase at 2 ~ 4 h and the late upregulating phase at 24 h post-irradiation. Overexpression of LPAR5 prompts EMT programing and migration of cancer cells. Moreover, increased expression of LPAR5 is significantly associated with IR-induced EMT and confers radioresistance to cancer cells. Knockdown of LPAR5 suppressed IR-induced EMT by attenuating the activation of ERK signaling and downstream Snail, MMP1, and MMP9 expression.

Conclusions
LPAR5 is an important upstream regulator of IR-induced EMT that modulates the ERK/Snail pathway. This study provides further insights into understanding the mechanism of radiation-induced EMT and identifies promising targets for improving the effectiveness of cancer radiation therapy.
Doppler study of portal vein and renal venous velocity predict the appropriate fluid response to diuretic in ICU: a prospective observational echocardiographic evaluation
Authors: Guinot Pierre-Grégoire , Bahr Pierre-Alain , Andrei Stefan , Popescu Bogdan A. , Caruso Vincenza , Mertes Paul-Michel , Berthoud Vivien , Nguyen Maxime , Bouhemad Belaid 
Published: October 2022

Abstract
Background
Fluid overload and venous congestion are associated with morbi-mortality in the ICU (intensive care unit). Administration of diuretics to correct the fluid balance is common, although there is no strong relationship between the consequent fluid loss and clinical improvement. The aim of the study was to evaluate the ability of the portal pulsatility index, the renal venous impedance index, and the VEXUS score (venous ultrasound congestion score) to predict appropriate diuretic-induced fluid depletion.

Methods
The study had a prospective, observational, single-center observational design and was conducted in a university-affiliated medico-surgical ICU. Adult patients for whom the clinician decided to introduce loop diuretic treatment were included. Hemodynamic and ultrasound measurements (including the portal pulsatility index, renal venous impedance index and VEXUS score) were performed at inclusion and 2 hours after the initiation of the diuretics. The patients’ characteristics were noted at inclusion, 24 h later, and at ICU discharge. The appropriate diuretic-induced fluid depletion was defined by a congestive score lower than 3 after diuretic fluid depletion. The congestive score included clinical and biological parameters of congestion.

Results
Eighty-one patients were included, and 43 (53%) patients presented with clinically significant congestion score at inclusion. Thirty-four patients (42%) had an appropriate response to diuretic-induced fluid depletion. None of the left- and right-sided echocardiographic parameters differed between the two groups. The baseline portal pulsatility index was the best predictor of appropriate response to diuretic-induced fluid depletion (AUC = 0.80, CI95%:0.70–0.92, p = 0.001), followed by the renal venous impedance index (AUC = 0.72, CI95% 0.61–0.84, p = 0.001). The baseline VEXUS score (AUC of 0.66 CI95% 0.53–0.79, p = 0.012) was poorly predictive of appropriate response to diuretic-induced fluid depletion.

Conclusion
The portal pulsatility index and the renal venous impedance index were predictive of the appropriate response to diuretic-induced fluid depletion in ICU patients. The portal pulsatility index should be evaluated in future randomized studies.
Associations of resting heart rate with incident dementia, cognition, and brain structure: a prospective cohort study of UK biobank
Authors: Deng Yue-Ting , Kuo Kevin , Wu Bang-Sheng , Ou Ya-Nan , Yang Liu , Zhang Ya-Ru , Huang Shu-Yi , Chen Shi-Dong , Guo Yu , Zhang Rui-Qi , Tan Lan , Dong Qiang , Feng Jian-Feng , Cheng Wei , Yu Jin-Tai 
Published: October 2022

Abstract
Background
Resting heart rate (RHR) has been linked with an increased risk of dementia. However, evidence characterizing the associations of RHR with different dementia subtypes and their underlying mechanisms remains scarce. This study aims to investigate the relationships of RHR with different dementia types, cognitive function, and brain structural abnormalities.

Methods
Three hundred thirty-nine thousand nine hundred one participants with no prior diagnosis of dementia from the UK biobank were analyzed. Cox regression and restricted cubic spline models examined the associations between RHR with all-cause dementia (ACD) and its major subtypes—Alzheimer’s disease (AD) and vascular dementia (VaD). Logistic regression models assessed the associations of RHR with cognitive function, and linear regression models estimated the associations with hippocampal subfield volume and white matter tract integrity indexed by magnetic resonance imaging data.

Results
During an average of 3148 (± 941.08) days of follow-up, 4177 individuals were diagnosed with dementia, including 2354 AD and 989 VaD cases. RHR ≥ 80bpm was associated with ACD (HR: 1.18, 95% CI: 1.08–1.28, P < 0.001) and VaD (HR: 1.29, 95% CI: 1.08–1.54, P = 0.005) but not AD in multi-adjusted models. A 10-bpm increment of RHR demonstrated non-linear effects in VaD, consisting of J-shape relationships. Several heterogeneities were indicated in stratified analysis, in which RHR measures only showed associations with dementia incidents in relatively younger populations (age ≤ 65) and females. Apart from dementia analysis, elevated RHR was associated with worsening performance in fluid intelligence and reaction time of cognitive tasks, decreased hippocampal subfields volume, and poor white matter tract integrity.

Conclusions
RHR is associated with increased risks of ACD and VaD but also presented with few heterogeneities across different sex and age groups. Elevated RHR also appears to have deleterious effects on cognitive function and is distinctively associated with volume reduction in hippocampal subfields and impaired white matter tract integrity.
Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions
Authors: Martínez-Álvaro Marina , Mattock Jennifer , Auffret Marc , Weng Ziqing , Duthie Carol-Anne , Dewhurst Richard J. , Cleveland Matthew A. , Watson Mick , Roehe Rainer 
Published: October 2022

AbstractBackgroundHealthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues. Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions). Possibly due to a lack of data, this strategy has never been explored.ResultsThis study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 orcis-9,trans-11C18:2 andtrans-11C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices. We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC). Of these, 372alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA. Of these 372alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy. These microbial genes were involved in microbial protein synthesis (aroFandserA), carbohydrate metabolism and transport (galT,msmX), lipopolysaccharide biosynthesis (kdsA,lpxD,lpxB), or flagellar synthesis (flgB,fliN) in certain genera within the Proteobacteria phyla (e.g.Serratia,Aeromonas). A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity). When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of −0.41±0.12 sd.ConclusionThis research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.