bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perturbation of
de novo
lipogenesis hinders MERS-CoV assembly and release, but not the biogenesis of viral replication organelles
Authors: Soultsioti M. , de Jong A. W. M. , Blomberg N. , Tas A. , Giera M. , Snijder E. J. , Bárcena M. 
Published: February 2025

ABSTRACT

Coronaviruses hijack host cell metabolic pathways and resources to support their replication. They induce extensive host endomembrane remodeling to generate viral replication organelles and exploit host membranes for assembly and budding of their enveloped progeny virions. Because of the overall significance of host membranes, we sought to gain insight into the role of host factors involved in lipid metabolism in cells infected with Middle East respiratory syndrome coronavirus (MERS-CoV). We employed a single-cycle infection approach in combination with pharmacological inhibitors, biochemical assays, lipidomics, and light and electron microscopy. Pharmacological inhibition of acetyl-CoA carboxylase (ACC) and fatty acid synthase (FASN), key host factors in
de novo
fatty acid biosynthesis, led to pronounced inhibition of MERS-CoV particle release. Inhibition of ACC led to a profound metabolic switch in Huh7 cells, altering their lipidomic profile and inducing lipolysis. However, despite the extensive changes induced by the ACC inhibitor, the biogenesis of viral replication organelles remained unaffected. Instead, ACC inhibition appeared to affect the trafficking and post-translational modifications of the MERS-CoV envelope proteins. Electron microscopy revealed an accumulation of nucleocapsids in early budding stages, indicating that MERS-CoV assembly is adversely impacted by ACC inhibition. Notably, inhibition of palmitoylation resulted in similar effects, while supplementation of exogenous palmitic acid reversed the compound’s inhibitory effects, possibly reflecting a crucial need for palmitoylation of the MERS-CoV spike and envelope proteins for their role in virus particle assembly.

IMPORTANCE
Middle East respiratory syndrome coronavirus (MERS-CoV) is the etiological agent of a zoonotic respiratory disease of limited transmissibility between humans. However, MERS-CoV is still considered a high-priority pathogen and is closely monitored by WHO due to its high lethality rate of around 35% of laboratory-confirmed infections. Like other positive-strand RNA viruses, MERS-CoV relies on the host cell’s endomembranes to support various stages of its replication cycle. However, in spite of this general reliance of MERS-CoV replication on host cell lipid metabolism, mechanistic insights are still very limited. In our study, we show that pharmacological inhibition of acetyl-CoA carboxylase (ACC), a key enzyme in the host cell’s fatty acid biosynthesis pathway, significantly disrupts MERS-CoV particle assembly without exerting a negative effect on the biogenesis of viral replication organelles. Furthermore, our study highlights the potential of ACC as a target for the development of host-directed antiviral therapeutics against coronaviruses.
Draft genome sequence of
“Burkholderia sola”
strains isolated in Mexico
Authors: Morales-Ruíz Leslie-Mariana , Larios-Serrato Violeta , Estrada-de los Santos Paulina 
Published: February 2025

ABSTRACT

This study presents the draft genomes of three strains of "
Burkholderia sola"
isolated from
Phaseolus vulgaris
L. trap plants and the rhizosphere of
Coffea arabica
L. in Mexico.
Complete genome sequences of
Yersinia pestis
6/69 strain isolated from a bubonic plague patient in Madagascar and its isogenic strain cured of pPCP1
Authors: Bougit Emelyne , Mas Fiol Guillem , Lê-Bury Pierre , Balière Charlotte , Caro Valérie , Pizarro-Cerdá Javier , Dussurget Olivier 
Published: February 2025

ABSTRACT

We report the complete genome sequences of two valuable strains to investigate plague pathogenesis: (i)
Yersinia pestis
strain 6/69, which was isolated from a bubonic plague patient in Madagascar and contains pCD1, pMT1, and pPCP1 virulence plasmids, and (ii) the 6/69 strain cured of pPCP1.
Genome assembly of
Ramulariopsis pseudoglycines
, a fungal pathogen responsible for areolate mildew on cotton
Authors: Garcia-Aroca Teddy , Kemerait Robert C. , Jimenez Madrid Alejandra M. 
Published: February 2025

ABSTRACT

The fungal species
Ramulariopsis pseudoglycines
was recently reported as a pathogen of cotton in the United States. Here, we report the first genome of this pathogen assembled using nanopore sequencing. The genome size is ~77.9 Mb, with an
N
50
of ~2.5 Mb, GC content of 52.74%, and Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 99.5%.
The impact of the social media industry as a commercial determinant of health on the digital food environment for children and adolescents: a scoping review
Authors: Lafontaine Jesse , Hanson Isabel , Wild Cervantée 
Published: February 2025

IntroductionThere is emerging evidence that the social media industry contributes to adverse health outcomes by shaping the digital food environment for children and adolescents (aged 0–18). The aim of this scoping review was to determine the extent of research on how the social media industry, including the broader online landscape, influences the digital food environment and affects child and adolescent health.MethodsA scoping review was conducted in the electronic databases of PubMed, Scopus and PsycINFO, along with forward and reverse citation searching for peer-reviewed articles published in English between 2000 and May 2023. A qualitative descriptive synthesis of the included articles was performed to identify trends, themes and research gaps in the current literature.ResultsThe review identified 36 articles for inclusion. Most research was conducted in high-income countries and publications have increased since 2021. The review found most children and adolescents are exposed to food advertisements on social media and most advertised food is ultra-processed. Heightened by a lack of social media advertising awareness, digital food marketing influences children and youth’s consumption and food behaviour. Voluntary children’s food marketing regulations are ineffective for the online environment. Countering unhealthy food marketing will require media literacy and government regulation.ConclusionThe social media industry may act as a commercial determinant of health to shape the digital food environment as an extension of the obesogenic environment. Further research should explore approaches to monitor unhealthy food marketing practices and understand social media’s role in the digital food environment.
Cost-effectiveness analysis of daratumumab plus bortezomib, melphalan and prednisone versus bortezomib, melphalan and prednisone in patients with newly diagnosed multiple myeloma ineligible for stem cell transplantation
Authors: Xie Dan , Zhang Peng-Fei 
Published: February 2025

ObjectivesThe aim of this study was to investigate the cost-effectiveness of bortezomib, melphalan and prednisone with daratumumab (D-VMP) versus bortezomib, melphalan and prednisone (VMP) in patients with newly diagnosed multiple myeloma (NDMM) who were ineligible for autologous stem-cell transplantation (ASCT).MethodsA Markov model was established and three exclusive health states, progression-free survival (PFS), progressive disease (PD) and death were included. The cycle length was set at 1.5 months and the time horizon was set at 20 years. The efficacy data were derived from the ALCYONE trial, and the utility values were obtained from previously published studies. The discount rate in the model was 3% per annum, and the willingness-to-pay (WTP) threshold was set at $150 000.00/quality-adjusted life year (QALY) in the USA. Moreover, one-way and probabilistic sensitivity analyses were performed to evaluate the parameter uncertainty.ResultsD-VMP achieved an effectiveness of 6.91 QALYs at a cost of $858 695.42, while VMP achieved an effectiveness of 4.70 QALYs at a cost of $357 387.82. The incremental effectiveness and cost of D-VMP versus VMP were 2.21 QALYs and $501 307.6, yielding an incremental cost-effectiveness ratio (ICER) of US $226 836.02/QALY. The cost of daratumumab, utility for PFS, cost of subsequent therapies in the VMP group and cost of subsequent therapies in the D-VMP group were the most influential factors, and the probabilities of D-VMP and VMP as the cost-effective option were 0% and 100% at the WTP threshold of $150 000.00/QALY, respectively.ConclusionsCompared with VMP, D-VMP is unlikely to be a cost-effective regimen for patients with NDMM who are ineligible for ASCT from the US payer’s perspective.
Is geographical variation in emergency epilepsy admissions related to variation in new epilepsy diagnoses among children and young people across England? An observational study using linked datasets
Authors: Shah Rakhee , Arora Sandeepa , Dunkley Colin , Raja Niky , Bottle Alex , Hargreaves Dougal S 
Published: February 2025

IntroductionPrevious studies have reported widespread unexplained geographical variations in emergency paediatric admissions for epilepsy.ObjectivesOur aim was to investigate the extent to which geographical variation in epilepsy admissions among children and young people (CYP) aged 0–18 years, in England, reflects variation in new epilepsy diagnoses.Design and settingA retrospective secondary analysis of Hospital Episode Statistics data for emergency admissions between April 2018 and March 2019, and Epilepsy12 audit data for new epilepsy diagnoses in England, between July and November 2018.Outcome measuresThe ratios of observed to expected epilepsy admissions and new diagnoses were calculated for each hospital Trust, based on their catchment population, and adjusted for age, sex and deprivation. Standardised ratios of observed to expected epilepsy admissions were plotted against standardised ratios of observed to expected new diagnoses of epilepsy at Trust level and the Pearson correlation coefficient was calculated.ResultsThere were 9246 emergency admissions for CYP to 134 Trusts with a primary diagnosis of epilepsy in England during the study period. 60 Trusts (44.4%) had either significantly lower or higher than expected standardised admission ratios for a primary diagnosis of epilepsy. There were 960 new diagnoses of epilepsy between July and November 2018 for 74 Trusts. 14 Trusts (18.9%) had either lower or higher standardised diagnosis ratios for a new diagnosis of epilepsy. There was no correlation between standardised emergency epilepsy admissions ratios and standardised new epilepsy diagnoses ratios at Trust level (Pearson r −0.06, p 0.63).ConclusionsWidespread unexplained variation in epilepsy admissions cannot be explained by variation in new epilepsy diagnosis. This raises concerns about the equity and accessibility of epilepsy services. Unit-level benchmarking data on new diagnoses and admissions for epilepsy can inform strategies to improve access to care and health outcomes.
Effectiveness and influencing factors of standardised training for nursing assistants: a cross-sectional study
Authors: Wu Ming , Yang Yanqing , Lin Yanqin , Chen Meizhen , Chen Ying 
Published: February 2025

ObjectivesTo investigate the impact of standardised training on nursing assistants (NAs) and explore the factors influencing training effectiveness, specifically focusing on learning motivation and learning obstacles.DesignA cross-sectional study was conducted from August 2022 to September 2022.SettingThe study was conducted in Fujian Province, China.ParticipantsA total of 412 participants were included in this study.Primary and secondary outcome measuresTraining effectiveness, learning motivation, learning obstacles and learning engagement were the primary outcome measures.MethodsA survey was conducted on research NAs who were participating in standardised training in Fujian Province, China. The survey used general information questionnaires, educational participation scales, general suitability scales for participation barriers, blended learning participation scales and training effectiveness questionnaires.ResultsThe overall score of the training effectiveness questionnaires was 98.08±15.52, with an average score per item of 4.26±0.67. Univariate analysis revealed significant associations between the number of working years, self-rated health status, acceptance of NAs work and training effectiveness (p<0.05). Multiple linear regression analysis indicated that learning motivation, learning obstacles and learning engagement were independent factors influencing training effectiveness (F=171.073, p<0.001).ConclusionLearning motivation, learning obstacles and learning engagement all affect training effectiveness, which provides a clear direction for improving the effectiveness of training for NAs. In order to improve the training effectiveness of healthcare aides, it is necessary for clinical managers to focus on their learning motivation, learning obstacles and learning engagement.
Designing vaping prevention messages for youth: a scoping review protocol
Authors: Chen Yu , Liu Shiyu , Cai Yujiang , Liu Haoyi , Zhang Rui , Yang Xinrui , Zhang Jinxiang , Jiang Youquan , Xu Jing , Yu Xinyao , Chan Kin-Sun 
Published: February 2025

IntroductionYouth vaping has become a significant public health concern globally. Effective prevention messages are essential to reduce the prevalence of vaping among young people. However, evidence on the design of youth vaping prevention messages is limited. This scoping review aims to systematically map the existing evidence, identify research gaps, and provide recommendations for future research and practice.Methods and analysisThe review will be conducted following the Joanna Briggs Institute methodology for scoping reviews and reported according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). A comprehensive search strategy has been developed focusing on the population-concept-context elements of youth, vaping, prevention, message design and global context. Six databases will be searched: Web of Science Core Collection (including MEDLINE), PubMed, Embase, Scopus, APA PsycInfo and China National Knowledge Infrastructure. Grey literature sources, including relevant organisational websites, will also be searched. Two reviewers will independently screen titles and abstracts, review full texts, and extract data using a predefined data charting form. The extracted data will be synthesised narratively.Ethics and disseminationAs this scoping review will only include published data and does not involve human participants, institutional review board approval is not required. The findings will be disseminated through peer-reviewed publications, conference presentations, academic networks, and social media.
Online physical activity resources for individuals with rheumatoid arthritis: an environmental scan and quality appraisal
Authors: Ester Manuel , Zafar Saania , Dhiman Kiran , Graveline Christine , McKinnon Annette , Hoens Alison , Barber Claire 
Published: February 2025

ObjectivesTo review publicly available physical activity (PA) resources for individuals with rheumatoid arthritis (RA). Aims were to find online print and audiovisual resources, review their characteristics and critically examine their quality from medical, exercise and behavioural science perspectives.DesignAn environmental scan was completed using the Google search engine, following a pragmatic approach to reviewing patient-facing self-care resources.Data sourcesWe used combinations of common search terms for RA and PA. The first five pages of results were reviewed for patient-facing resources.Eligibility criteriaResources were included if they were (1) included RA-specific content, (2) provided specific PA recommendations, (3) written in English and (4) freely available.Data extraction and synthesisTwo independent experts completed a medical review of resources to ensure appropriateness for RA. Data were then extracted by two reviewers using a standardised template to record resource characteristics. Two research team members and two patient partners independently evaluated resources for readability, understandability and actionability. Finally, the quality of exercise recommendations and behaviour change technique use was evaluated by an expert reviewer.ResultsThe search yielded 23 RA-specific PA resources, 17 of which passed the medical review. All 10 print resources and 7 audiovisual resources were created in English-speaking countries. The mean reading grade was 9.0±1.5. Print resources had mean understandability of 80.0±9.8% and actionability of 60.0±27.7%. Audiovisual materials had mean understandability of 86.0±9.2% and actionability of 86.9±22.9%. The quality of exercise recommendations was low. Only one resource provided comprehensive cardiovascular exercise advice, and two resources provided comprehensive strength exercise advice. 3–14 behaviour change technique groups were featured in each resource. The most common groups were ‘shaping knowledge’ and ‘natural consequences’.ConclusionsThe quality of RA-specific PA resources is variable. Some high-quality resources exist that provide actionable PA behaviour change advice. Healthcare teams may refer patients to these resources. However, more work is needed to improve the overall quality of resources. Codevelopment with patients, providers and exercise behaviour change experts is recommended, ensuring resources are actionable, contain clear exercise recommendations and promote behaviour change.
Changes in global quality of life after treatment with immune checkpoint inhibitors in patients receiving different treatment regimens for advanced stage lung cancer in the Netherlands: a 2015–2021 cohort study
Authors: Malhotra Ananya , Suazo-Zepeda Erick , Vinke Petra C , de Bock Geertruida H , Maas Willemijn J , Hiltermann Jeroen T J.N , Rachet Bernard , Leyrat Clémence , Quaresma Manuela 
Published: February 2025

BackgroundThe introduction of immune checkpoint inhibitors (ICIs) has modified treatment modalities for patients with lung cancer, offering new alternatives for treatment. Despite improved survival benefits, ICIs may cause side effects impacting patients’ quality of life (QoL). We aim to study the changes in global QoL (gQoL) of patients with advanced-stage lung cancer up to 18 months after treatment with ICIs between 2015 and 2021.Methods and analysisA longitudinal cohort study was conducted using the Oncological Life Study: Living well as a cancer survivor data-biobank from the University Medical Center Groningen. Participants completed the European Organisation for Research and Treatment of Cancer QoL 30-item questionnaire, at the beginning of their ICI treatment (baseline) and then at 6, 12 and 18 months. Using joint modelling, changes in predicted mean gQoL were studied by treatment regimens from baseline to 18 months, while accounting for the competing risk of death and adjusting for prespecified covariates.ResultsOf the 418 participants with median age of 66 years, 39% were women. Patients receiving first-line immuno-monotherapy with palliative intent had a small improvement in their gQoL within 6 months and no clinically significant change thereafter. Patients receiving first-line immune-chemotherapy with palliative intent had a small improvement in their gQoL within 12 months and no clinically significant change thereafter. Patients with second/further line immunotherapy with palliative intent or first-line chemoradiotherapy followed by durvalumab with curative intent had no clinically significant change in their gQoL over 18 months.ConclusionThe changes in gQoL over time among patients with advanced-stage lung cancer may vary by treatment regimens based on drug intensity, line and intent of treatment, which will help clinicians and patients understand the potential dynamic of treatments on QoL. It may further influence treatment decisions and patient management strategies, reflecting the practical implications of different treatment regimens.
‘The lights are on, and the doors are always open’: a qualitative study to understand challenges underlying the need for emergency care in people experiencing homelessness in rural and coastal North East England
Authors: Thirkle Steven A , Adams Emma A , Harland Jill , John Deepti A , Kaner Eileen , Ramsay Sheena E 
Published: February 2025

IntroductionPeople experiencing homelessness have high rates of emergency care attendance compared with the general population. This study explores the factors underlying the need for emergency care services among people experiencing homelessness in rural and coastal areas of North East England.MethodsThe study was conducted in Northumberland and North Tyneside (North East England). One-to-one semistructured interviews were conducted with people experiencing homelessness. Interviews and focus groups were undertaken with frontline staff from housing associations, police, ambulance services, emergency care, primary healthcare, mental health services and alcohol and drug recovery services. Discussions centred on emergency care experiences, reasons for access and underlying health and social needs.ResultsParticipants included 20 people experiencing homelessness (aged 18–56, 70% male) and 18 service professionals (aged 20–56, 56% female). Emergency care was often viewed by participants as an accessible safe place. Four key themes were found in this rural and coastal context: accessibility challenges due to limited public transport and geographic isolation; fragmented support exacerbated by widely dispersed services; service restrictions and limited alternatives having particular impact where options are few and prioritisation of immediate needs influenced by limited local resources.ConclusionChallenges in accessing primary healthcare and social care, alongside varying levels of timely support and understanding of individual contexts, can contribute to the increased use of emergency care for people experiencing homelessness in rural and coastal areas. Integrating services with a focus on flexibility could be crucial for addressing the needs of these populations. This involves adapting to the unique circumstances of multiple deprived groups who lack access to community support.