bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Coagulation Mechanism in the Development of Acute Thrombosed Hemorrhoidal Disease
Authors: ALKAN Selman , ÇAKIR Murat , VARMAN Alper , SENTÜRK Mustafa , KİSİ Omer , TOPAL Ahmet 
Published: November 2022

ABSTRACT
Background Hemorrhoidal disease is a common benign anorectal disease. Acute thrombosis that occurs during the course of hemorrhoidal disease is a painful complication. Factors affecting its etiopathogenesis are not known definitively. The aim of this study, therefore, was to assess predisposition to coagulation during the development of the disease.
Method 30 patients with acute thrombosed hemorrhoidal disease and 30 other patients with hemorrhoidal disease but no thromboses were included in the study. Samples collected from
these patients were analyzed with thromboelastography machines. The results were compared
with patients’ demographic data.
Results No statistically significant difference was found between the groups as per age, sex, diarrhea, history of a similar attack, history of surgical treatment, spicy food consumption, fibrous food consumption, and regular exercise. The results of our study revealed that the alpha angle was smaller in patients with acute
thrombosed hemorrhoidal disease.
Conclusion Hypercoagulability does not occur in patients with acute thrombosed hemorrhoidal disease
Minimal residual disease in B-precursor acute lymphoblastic leukemia: Clinical significance on post-induction treatment
Authors: Shervashidze M. A. , Valiev T. T. , Palladina A. D. , Kirgizov K. I. , Tupitsyn N. N. 
Published: November 2022

Minimal residual disease (MRD) assessment in patients with acute lymphoblastic leukemia (ALL) became an integral part of total evaluation of chemotherapy efficacy. No doubt, that it is necessary to evaluate MRD at remission induction and before allogenic stem cell transplantation, but MRD prognostic role at post-induction treatment is on-study.Aim. To evaluate a prognostic significance of MRD at post-induction treatment in pediatric patients with ALL.Materials and methods. From October 2010 to September 2022, there were 142 pediatric patients with primary diagnosed B-precursor ALL enrolled the study. All the patients were treated by ALL–IC BFM 2009 protocol.Results. 6-year overall survival (OS) for patients with MRD-positive status at post-induction treatment (78th day of therapy) was 90.8 ± 4.0 %, with MRD-negative – 91,1 ± 3.9 % (р = 0,8). Relapse-free survival (RFS) for MRD-positive – 67.4 ± 11.6 %, MRD-negative – 88.9 ± 4.3 % (р = 0,03). Event-free survival for MRD-positive – 67.4 ± 11.6 %, MRD-negative – 87,5 ± 4.5 % (р = 0,06).Conclusion. MRD level at induction remission treatment is an important risk-stratified factor in pediatric patients with ALL, but prognostic significance of MRD at post-induction therapy is under investigation. Presented our data showed increased relapse incidence on 21.5 % in patients with MRDpositive status at 78th day of therapy. OS was the same and not depended on post-induction MRD-level.
Association between migraine and cardiovascular disease: A cross-sectional study
Authors: Wang Kai , Mao Yukang , Lu Miao , Ding Yinzhang , Li Zhongming , Li Yansong , Liu Xianling , Sun Yan , Hong Jian , Xu Di , Wu Tingting 
Published: November 2022

BackgroundCardiovascular disease (CVD) poses a tremendous threat to global health, giving rise to exceedingly high morbidity and mortality among patients. A migraine is a common neurological disorder characterized by recurrent attacks of severe headache, while its cardiovascular burden remains unclear. Therefore, this study aims to investigate whether migraine is associated with CVD.MethodsThe cross-sectional data of 5,692 subjects aged 20 or above was obtained from the National Health and Nutrition Examination Survey (NHANES) 1999–2004. To determine whether migraine is associated with CVD, weighted logistic regression models were used. In a subsequent subgroup analysis, several confounding factors were also explored to investigate the association between migraine and CVD.ResultsIn total, 5,692 subjects were enrolled in this study, with the prevalence of CVD being 13.3%. Participants with CVD tended to be older, male, non-Hispanic whites, more educated, former smokers, and alcohol drinkers, and had a higher waist circumference, less physical activity, a higher level of triglyceride and creatinine as well as a lower level of high-density lipoprotein cholesterol (HDL-C) and estimated glomerular filtration rate (eGFR) (all P &lt; 0.05). Considering all potential confounders, migraine was associated with a higher risk of CVD [odds ratios (ORs) 2.77; 95% confidence intervals (CIs): 1.56–4.90]. Subgroup analysis showed a higher risk of CVD in females, those older than 60 years, with a lower body mass index (BMI) level (≤ 30 kg/m2), a higher level of eGFR (&gt; 90 mL/min/1.73 m2), hypertension and hyperlipidemia and without diabetes.ConclusionIn summary, our study revealed a positive association between migraine with CVD in a nationally representative US adult population. Our findings highlighted that migraine should be considered an important risk factor for CVD.
Temperature-controlled ablation of the mitral isthmus line using the novel DiamondTemp ablation system
Authors: Rottner Laura , My Ilaria , Schleberger Ruben , Moser Fabian , Moser Julia , Kirchhof Paulus , Ouyang Feifan , Rillig Andreas , Metzner Andreas , Reissmann Bruno 
Published: November 2022

BackgroundThe novel DiamondTemp™ (DT)-catheter (Medtronic®) was designed for high-power, short-duration ablation in a temperature-controlled mode.AimTo evaluate the performance of the DT-catheter for ablation of the mitral isthmus line (MIL) using two different energy dosing strategies.Materials and methodsTwenty patients with recurrence of atrial fibrillation (AF) and/or atrial tachycardia (AT) following pulmonary vein (PV) isolation were included. All patients underwent reisolation of PVs in case of electrical reconnection and ablation of a MIL using the DT-catheter. Application durations of 10 (group A, n = 10) or 20 s (group B, n = 10) were applied. If bidirectional block was not reached with endocardial ablation, additional ablation from within the coronary sinus (CS) was conducted.ResultsIn 19/20 (95%) patients, DT ablation of the MIL resulted in bidirectional block. Mean procedure and fluoroscopy time, and dose area product did not differ significantly between the two groups. In group B, fewer radiofrequency applications were needed to achieve bidirectional block of the MIL when compared to group A (26 ± 12 vs. 42 ± 17, p = 0.04). Ablation from within the CS was performed in 8/10 patients (80%) of group A and in 5/10 (50%) patients of group B (p = 0.34). No major complication occurred.ConclusionMitral isthmus line ablation with use of the DT-catheter is highly effective and safe. Longer radiofrequency-applications appear to be favorable without compromising safety.
A web-based predictive model for overall survival of patients with cutaneous Merkel cell carcinoma: A population-based study
Authors: Xu Wen , Le Yijun , Zhang Jianzhong 
Published: November 2022

BackgroundMerkel cell carcinoma (MCC) is an aggressive neuroendocrine carcinoma with a high mortality rate, so it is necessary to create models to predict overall survival of MCC. We developed an easy-to-use web-based calculator to predict the OS of MCC patients based on the nomogram.MethodsMCC patients between 2004 and 2015 were collected from the Surveillance, Epidemiology, and End Results (SEER) database and randomly assigned to training and validation cohorts. Patients between 2016-2017 serve as the external validation cohort. Relevant risk factors were identified by univariate and multivariate COX hazards regression methods and combined to produce nomograms. The concordance index (C-index), area under the receiver operating characteristic (AUC) curve, and calibration plots have demonstrated the predictive power of the nomograms. Decision curve analysis (DCA) was used to measure nomograms in clinical practice. Patients were divided into three groups according to the scores of the nomogram.ResultsA total of 3480 patients were randomly assigned to the training group and validation group in this study. Meaningful prognostic factors were applied to the establishment of nomograms. The C-index for OS was 0.725 (95% CI: 0.706-0.741) in the training cohort and 0.710 (95% CI: 0.683-0.737) in the validation cohort. In the external validation cohort, C-index was 0.763 (95% CI: 0.734–0.792). The C-index of training cohort, validation cohort and external validation cohort for CSS were 0.743 (95% CI:0.725-0.761), 0.739(95%CI:0.712-0.766) and 0.774 (95%CI:0.735-0.813), respectively. The AUC and calibration plots of 1-, 3-, and 5-year OS rates showed that the nomogram had good predictive power. DCA demonstrated that the nomogram constructed in this study could provide a clinical net benefit. Our calculator demonstrated excellent predictive capabilities for better risk grouping of MCC patients.ConclusionWe created novel nomograms of prognostic factors for MCC, which more accurately and comprehensively predicted 1-, 3-, and 5-year OS/CSS in MCC patients. We established a calculator which can easily and quickly calculate the risk grouping of MCC patients by inputting clinically relevant characteristics. This can help clinicians identify high-risk patients as early as possible, carry out personalized treatment, follow-up, and monitoring, and improve the survival rate of MCC patients.
Detection of ampicillin based on the fluorescence of a biocatalytic oxidation product
Authors: García-Zamora José Luis , Alonso-Arenas Jessica , Rebollar-Pérez Georgette , Pacheco-Aguirre Francisco M. , García-Diaz Esmeralda , Torres Eduardo 
Published: November 2022

Antibiotics used in humans and farmed animals are an essential source of water and soil contamination. Ampicillin is a micropollutant commonly found in water, sludge, food, flora, and fauna. However, the methods used for its detection in environmental samples are often complicated and expensive. Therefore, developing more straightforward strategies to detect well-known target antibiotics is necessary. In this context, enzyme-based detection methods have been demonstrated to be selective, sensitive, rapid, and relatively simple. In this study, a fluorescent byproduct from the ampicillin oxidation using Chloroperoxidase (CPO) enzyme was used as a pointer compound to determine ampicillin concentration in environmental water samples. We oxidized 80% ampicillin for 1h, producing a fluorescent compound with m/z 274.2517. A response surface methodology (RSM) based on a central composite design (CCD) was used to evaluate and optimize the effects of hydrogen peroxide, enzyme concentration, and time as independent variables on the maximum fluorescence signal as the response function. The methodology proposes to build a calibration curve that relates the initial concentration of ampicillin with fluorescence intensity after the reaction with CPO, which helps detect ampicillin in the concentration range from 0.035 to 40 μM, with a limit of detection of 0.026 μM. The application of the method to fortified environmental water samples allowed percentages of recovery from 86 to 140%. The formation of the fluorescent compound was not affected by the presence of salts commonly found in wastewater; however, it was affected by other antibiotics. The proposed methodology was tested in the context of water from water bodies, urban, and WWTP effluents.
Identification of biomarkers associated with CD8+ T cells in rheumatoid arthritis and their pan-cancer analysis
Authors: Zhao Zhenyu , Ren Jie , Xie Siping , Zou Longchun , Zhao Qianyue , Zeng Shan , Zha Dingsheng 
Published: November 2022

IntroductionRheumatoid arthritis (RA), a prevailing chronic progressive autoimmune disease, seriously affects the patient's quality of life. However, there is still a lack of precise treatment and management methods in clinical practice. Previous studies showed that CD8+ T cells take a lead in the progression of RA.MethodsGenes closely related to CD8+T cells in RA were identified through multiple RA datasets, CIBERSORT, and WGCNA algorithms. Further machine learning analysis were performed to identify CD8+T cell-related genes most closely related to RA. In addition, the relationship between these three key genes and 33 cancer species was also explored in this study.ResultsIn this study, 10 genes were identified to be closely related to CD8+T cells in RA. Machine learning analysis identified 3 CD8+T cell-related genes most closely related to RA: CD8A, GZMA, and PRF1.DiscussionOur research aims to provide new ideas for the clinical treatment of RA.
Atorvastatin and ezetimibe protect against hypercholesterolemia-induced lung oxidative stress, inflammation, and fibrosis in rats
Authors: Seenak Porrnthanate , Kumphune Sarawut , Prasitsak Thanit , Nernpermpisooth Nitirut , Malakul Wachirawadee 
Published: November 2022

BackgroundHypercholesterolemia is a major risk factor for interstitial lung disease (ILD). Atorvastatin and ezetimibe are antilipemic drugs that have pleiotropic effects. However, their effects on pulmonary fibrosis prevention and the mechanisms underlying hypercholesterolemia have not been fully investigated. This study aimed to evaluate the individual effects of atorvastatin and ezetimibe on lung inflammation and fibrosis in high-cholesterol diet (HCD)-fed rats.Materials and methodsMale Sprague-Dawley rats were divided into four groups — standard diet (S), standard diet + 1% cholesterol (SC), standard diet + 1% cholesterol with 30 mg/kg/day atorvastatin (SCA), and standard diet + 1% cholesterol with 10 mg/kg/day ezetimibe (SCE). At the end of an 8-week dietary schedule, serum lipid parameters and the levels of lung oxidative stress, inflammatory cytokines, and fibrotic mediators were determined.ResultsAtorvastatin and ezetimibe treatment remarkably reduced serum lipid profiles with reversed pulmonary histological alterations, in addition to reducing the levels of lung oxidative stress, inflammation, and fibrosis in hypercholesterolemic rats.ConclusionAtorvastatin and ezetimibe treatment showed a protective effect against hypercholesterolemia-induced pulmonary fibrosis in rats. This information appears potentially useful in the prevention of PF in a hypercholesterolemia model; however, further rigorous investigations are needed to prove their clinical utility on antifibrosis.
Epichloë bromicola from wild barley improves salt-tolerance of cultivated barley by altering physiological responses to salt stress
Authors: Wang Zhengfeng , Liu Jing , White James F. , Li Chunjie 
Published: November 2022

IntroductionEpichloë bromicola is a cultivable fungal endophyte that lives in symbiosis with wild barley (Hordeum brevisubulatum) to which it confers salt tolerance. This study tested the hypothesis that E. bromicola derived from wild barley has the potential to increase salt tolerance in cultivated barley under salt stress.MethodsTo test this hypothesis, the growth response, physiological parameters, and metabolic profiles of barley plants inoculated with E. bromicola (E+) and those not inoculated with E. bromicola (E–) were compared under salt stress.ResultsCompared with E– barley plants, E+ barley plants had significantly increased plant height, shoot biomass, total biomass, chlorophyll content, osmotic synthesis, and accumulation of stress adaptation metabolites. E. bromicola increased the salt stress tolerance of cultivated barley, and the positive effects correlated with different salt stress conditions.DiscussionThese results suggest that E. bromicola has promising potential for enhancing the salt tolerance of barley. New insights into the mechanisms underlying this barley–fungal endophyte association are provided, and interesting questions regarding the role of E. bromicola in fungus-enhanced tolerance to salt stress in this symbiosis are raised.
Effect of sera from elite athletes on cytokine secretion and insulin signaling in preadipocytes and skeletal muscle cells
Authors: Alheidous Sara , Al-Muraikhy Shamma , Rizk Nasser , Sellami Maha , Donati Francesco , Botre Francesco , Al-Mansoori Layla , Elrayess Mohamed A. 
Published: November 2022

Introduction: The immunomodulatory effect of physical activity can impact insulin signaling differentially in adipose tissues and skeletal muscle cells, depending on sport intensity. In this study, the effect of serum from elite athletes with varying endurance levels and playing different power sports on cytokine secretion and insulin signaling in preadipocyte and skeletal muscle cell lines was investigated.Methods: Preadipocytes (3T3-L1) and skeletal muscle cells (C2C12) were cultured in media containing pooled sera from elite athletes who play high-endurance (HE), high-power (HP), or low-endurance/low-power (LE/LP) sports for 72 h. Secreted cytokines (IL-6 and TNF-alpha) were assessed in the supernatant, and insulin signaling phosphoproteins levels were measured in lysates following treatment using cells multiplex immunoassays.Results: Sera from LE/LP and HP induced TNF-α secretion in C2C12, while serum from HE reduced IL-6 secretion compared to non-athlete serum control. All elite athlete sera groups caused decreased insulin sensitivity in 3T3-L1 cells, whereas in C2C12 cells, only HE athlete serum reduced insulin signaling, while LE/LP and HP caused increased insulin sensitivity.Conclusion: Sera from elite athletes of different sport disciplines can affect the inflammatory status and insulin signaling of preadipocytes and myoblasts differently, with risk of developing insulin resistance. Furthermore, investigation of the functional relevance of these effects on exercise physiology and pathophysiology is warranted.
Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats
Authors: Guha Suman K. , Alonso-Caraballo Yanaira , Driscoll Gillian S. , Babb Jessica A. , Neal Megan , Constantino Nicholas J. , Lintz Tania , Kinard Elizabeth , Chartoff Elena H. 
Published: November 2022

IntroductionRates of relapse to drug use during abstinence are among the highest for opioid use disorder (OUD). In preclinical studies, reinstatement to drug-seeking has been extensively studied as a model of relapse–but the work has been primarily in males. We asked whether biological sex contributes to behaviors comprising self-administration of the prescription opioid oxycodone in rats, and we calculated the relative contribution of these behavioral measures to reinstatement in male and female rats.Materials and methodsRats were trained to self-administer oxycodone (8 days, training phase), after which we examined oxycodone self-administration behaviors for an additional 14 days under three conditions in male and female rats: short access (ShA, 1 h/d), long access (LgA, 6 h/d), and saline self-administration. All rats were then tested for cue-induced reinstatement of drug-seeking after a 14-d forced abstinence period. We quantified the # of infusions, front-loading of drug intake, non-reinforced lever pressing, inter-infusion intervals, escalation of intake, and reinstatement responding on the active lever.ResultsBoth male and female rats in LgA and ShA conditions escalated oxycodone intake to a similar extent. However, males had higher levels of non-reinforced responding than females under LgA conditions, and females had greater levels of reinstatement responding than males. We then correlated each addiction-related measure listed above with reinstatement responding in males and females and ranked their respective relative contributions. Although the majority of behavioral measures associated with oxycodone self-administration did not show sex differences on their own, when analyzed together using partial least squares regression, their relative contributions to reinstatement were sex-dependent. Front-loading behavior was calculated to have the highest relative contribution to reinstatement in both sexes, with long and short inter-infusion intervals having the second greatest contribution in females and males, respectively.DiscussionOur results demonstrate sex differences in some oxycodone self-administration measures. More importantly, we demonstrate that a sex- dependent constellation of self-administration behaviors can predict the magnitude of reinstatement, which holds great promise for relapse prevention in people.
Loxhd1b inhibits the hair cell development in zebrafish: Possible relation to the BDNF/TrkB/ERK pathway
Authors: Liu Jingwen , Zhang Xu , Zhang Qingchen , Wang Rongrong , Ma Jingyu , Bai Xiaohui , Wang Dawei 
Published: November 2022

BackgroundMutations in lipoxygenase homology domain 1 (LOXHD1) cause autosomal recessive inheritance, leading to high-frequency and intermediate-frequency hearing losses in patients. To date, studies on the localization of LOXHD1 gene expression are limited. In this study, we aimed to observe the expressions of Loxhd1b in zebrafish, C57BL/6 murine cochlea, and HEI-OC1 cells.MethodsThe expression of Loxhd1b in the auditory system of zebrafish was explored by in situ hybridization experiments of zebrafish embryos. The expression of Loxhd1b in cochlear and HEI-OC1 cells of C57BL/6 mice was analyzed by immunofluorescence staining. Confocal microscopic in vivo imaging was used to detect the number and morphological characteristics of lateral line neuromasts and inner ear hair cells in zebrafish that knocked down Loxhd1b gene. The effect of knockdown Loxhd1b gene on the development of zebrafish otolith and semicircular canal was observed using microscopic. Transcriptome sequencing was used to identify downstream molecules and associated signaling pathways and validated by western blotting, immunostaining, and rescue experiments.ResultsResults of the in situ hybridization with zebrafish embryos at different time points showed that Loxhd1b was expressed in zebrafish at the inner ear and olfactory pores, while the immunostaining showed that Loxhd1 was expressed in both C57BL/6 mouse cochlea and HEI-OC1 cells. Loxhd1b knockdown causes a decrease in the number of spinal and lateral line neuromasts in the inner ear of zebrafish, accompanied by weakened hearing function, and also leads to developmental defects of otoliths and ear follicles. The results of transcriptomics analysis revealed the downstream molecule brain-derived neurotrophic factor (BDNF) and verified that Loxhd1b and BDNF regulate the formation of zebrafish hair cells by synergistic regulation of BDNF/TrkB/ERK pathway based on western blotting, immunostaining, and rescue experiments.ConclusionThis was the first time that the BDNF/TrkB/ERK pathway was identified to play a critical role in the molecular regulation of the development of zebrafish hair cells and the auditory development by Loxhd1b.