bluuebunny/crossref_metadata_2025_split huggingface dataset:
Site-specific seismic hazard and risk potential of Bengal Basin with emphasis on holistic seismic hazard microzonation and its structural impact assessment in the cities of Dhanbad and Mymensingh
Authors: Nath Sankar Kumar , Biswas Arpita , Srivastava Anand , Sengupta Arnab , Ghatak Chitralekha , Madan Jyothula 
Published: August 2022

The Bengal Basin located in the eastern part of the Indian subcontinent at the conjunction of the Eurasian, Indian, and Indo-Burma plates with two progressing deformation fronts viz. the Himalayas and the Indo-Burmese orogenic belts is one of the largest fluvio-deltaic to shallow marine sedimentary basin covered by alluvial plains of Holocene deposits extending from the Himalayas to the Bay of Bengal over thick younger alluvium comprising shallow layers of silt, clay, and sand that can have disastrous consequences due to site-specific ground motion amplification and liquefaction effects. The basin surrounded by Shillong and Assam plateaus in the Northeast is in the active tectonofabric of major active faults and lineaments triggering many devastating earthquakes in the past implicating the MM Intensity of VIII–XI in the near-source region causing widespread damage and destruction in the basin, thus bringing in the essence of assessing surface level seismic hazard and the risk imposed on the basin. Consideration of seismicity patterns, fault networks, and similarity in focal mechanisms yielded 49 areal seismogenic sources and additional active tectonic features in the 0–25 km, 25–70 km, and 70–180 km hypocentral depth ranges, which along with 14 ground motion prediction equations that include site-specific next generation spectral attenuation models pertaining to Northeast India, East-Central Himalaya, and Bengal Basin tectonic provinces yielded probabilistic peak ground acceleration (PGA) at engineering bedrock in the range of 0.08–0.58 g. Both the geophysical and geotechnical investigations at 6,000 sites provided effective shear wave velocity distribution in the range of 113–948 m/s on the geographical information system, thus classifying the basin into 11 site classes with “None” to “Severe” liquefaction hazard potential. A systematic non-linear/equivalent linear site response analysis and its spectral convolution with firm rock peak ground acceleration yielded surface-consistent hazard in the range of 0.09–1.17 g, thus opening up the issue of risk assessment and holistic seismic hazard microzonation of all the cities in the basin and their structural impact assessment using the SELENA-based capacity spectrum method on FEMA and BMTPC-regulated 11 model building types in the damage states of “none,” “slight,” “moderate,” “extensive,” and “complete” for all of those, however, in-depth studies carried out for Mymensingh and Dhanbad have been presented.
Living coralligenous as geo-historical structure built by coralline algae
Authors: Basso Daniela , Bracchi Valentina Alice , Bazzicalupo Pietro , Martini Marco , Maspero Francesco , Bavestrello Giorgio 
Published: August 2022

The most important reef of the Mediterranean is the Coralligène (Coralligenous = C), including several types of calcareous algal-invertebrate build-ups growing in normal open marine conditions. We analyzed and compared two C samples from the Ligurian Sea developed in different environmental settings: 1) off Portofino on a rocky cliff, at a depth of about 40 m and 2) in front of Bogliasco, on a sub-horizontal substrate at a depth of 10 m. The maximum AMS radiocarbon dating provided an older age for Bogliasco (about 5 ka BP) than for Portofino (about 3.6 ka BP), and the mean accumulation rate of the Portofino build-up (about 80 µm y−1) was found to be higher than the one in Bogliasco (about 65 µm y−1). The different sides of each build-up showed a remarkable heterogeneity in the dominant cover by living organisms, and the comparison between the two build-ups highlighted an evident diversity in their taxonomic composition and structure, although crustose coralline algae (CCA) are the dominant framework builder and major autogenic ecosystem engineers at both localities, in the present as in the past millennia. Other major components of the structure are bryozoans and serpulids, and an important role is played by sediment filling. In Bogliasco, extreme climate events and major peaks of fine matrix and terrigenous grains are observed, lithologically related to the drainage basin of the Poggio creek and associated with charophyte occurrence and reduced CCA abundance. The occurrence of the rareSporolithon ptychoideswas observed both in Portofino at about 750 BCE and in Bogliasco. TheseSporolithonphases are likely related to warm and humid spells punctuating the Holocene climate fluctuations in the Ligurian Sea. Because coralline algae are confirmed to be the most important habitat engineer of the Mediterranean reefs, they deserve more attention in the framework of any monitoring initiative aimed at C management and conservation.
Subseasonal forecasts of precipitation over maritime continent in boreal summer and the sources of predictability
Authors: Lyu Yang , Zhu Shoupeng , Zhi Xiefei , Dong Fu , Zhu Chengying , Ji Luying , Fan Yi 
Published: August 2022

In this study, subseasonal precipitation forecast skills over Maritime Continent in boreal summer are investigated for the ECMWF and CMA models involved in the S2S Project. Results indicate that the ECMWF model shows generally superior forecast performances than CMA, which is characterized by lower errors and higher correlations compared with the observations. Meanwhile, ECMWF tends to produce wet biases with increasing lead times, while the mean errors of CMA are revealed to be approximately constant throughout lead times of 2–4 weeks over most areas. Besides, the temporal correlations between model outputs and observations obviously decrease with growing lead times, with a high-low distribution presented from north to south. In addition, the roles of large-scale drivers like ENSO and BSISO in modulating subseasonal precipitation forecast skills are also assessed in the models. Both ECMWF and CMA can reasonably capture the ENSO related precipitation anomalies for all lead times, while their capabilities of capturing BSISO related precipitation anomalies decrease with growing lead times, which is more obvious in CMA. The enhanced subseasonal precipitation forecast skills mainly respond to the BSISO associated precipitation variability. For most MC areas such as southern Indochina, western Indonesia, Philippines and the eastern ocean, the forecast skills of both ECMWF and CMA can be improved to a great extent by enhancing the capture of BSISO related precipitation anomalies, with the temporal correlations for both ECMWF and CMA increased by about 0.15 for lead times of 3–4 weeks. It provides an opportunity window for the models to improve precipitation forecasts on the subseasonal timescale.
The burden of vitamin D deficiency in household members of children presenting with symptomatic vitamin D deficiency
Authors: Uday Suma , Högler Wolfgang 
Published: August 2022

BackgroundMicronutrient deficiencies are common among household/family members due to shared lifestyle and dietary habits. The extent of biochemical abnormalities in household members of children presenting with symptomatic vitamin D deficiency remains unknown.AimInvestigate the prevalence of vitamin D deficiency and biochemical osteomalacia in the mothers and siblings of children presenting with symptomatic vitamin D deficiency.MethodsAll mothers and sibling of children referred to a single tertiary endocrine centre between January 2018 and December 2021, with symptomatic vitamin D deficiency were investigated prospectively for vitamin D deficiency [defined as 25 hydroxyvitamin D (25OHD) &lt; 30nmol/L] and biochemical osteomalacia [vitamin D deficiency and elevated alkaline phosphatase (ALP) and/or parathormone (PTH)] as per clinical guidelines.ReultsNinety-seven family members (68 siblings and 29 mothers) of 29 index cases (median age 1.7 years, 55.5% male) were investigated. The majority (65.5%, n=19) were of Asian ethnic background. The mean (SD) 25OHD levels of the index, maternal and sibling cohorts were 15 (10), 15 (7) and 20 (10) nmol/L respectively. Vitamin D deficiency was noted in 93% of the maternal and 79% of the sibling cohorts. Biochemical osteomalacia was present in 72% of the maternal and 79% of the sibling cohorts. Mothers of infants had significantly lower mean 25OHD levels compared to mothers of older children [11 (n=12) vs 18 nmol/L (n=17) respectively, p=0.006)], most of whom were symptomatic (66.6%, n=8/12). None of the mothers had hypocalcaemia. Among the 10% (n=7) of the siblings with hypocalcaemia, 86% (n=6/7) had concurrent dietary calcium deficiency and 71.4% (n= 5/7) reported symptoms in retrospect. Hypocalcaemic siblings had significantly lower 25OHD (7 vs 15 nmol/L, p&lt;0.001), higher PTH (175 vs 58 ng/L, p&lt;0.001) and ALP (846 vs 318 IU/L, p&lt;0.001), respectively compared to normocalcaemic siblings.ConclusionsIn view of the substantial morbidity uncovered in household/family members of children diagnosed with symptomatic vitamin D deficiency, we recommend universal supplementation of all risk groups. Biochemical testing and treatment is indicated to replenish stores only in those at highest risk such as mothers of infants, individuals with concurrent dietary calcium deficiency and those with clinical symptoms.
A feasibility study of SMART reactor power performance optimizations-part 1: Steady-state and burn-up analysis
Authors: Zhong Yiming , Norman Paul , Wu Wenbin 
Published: August 2022

SMART is an integral small pressurized water nuclear reactor design with a rated power output of 100 MWe from 330 MWth, but it needs a higher power output for the United Kingdom energy market. This study applies Monte Carlo code OpenMC to build a full-core model and innovatively adjust the simulation coefficients to approach the reactor operating conditions. The analysis results point out the reasonable optimization’s technical direction. The model’s sensitivity to ENDF and JEFF nuclear data libraries and spatial division is tested and verified. Then it performs a series of simulations to obtain the core’s neutronic parameters, such as neutron energy and spatial distributions, effective neutron multiplication factor keff and its variation versus depletion. The analysis found that the initially designed core’s keff is 1.22906, and the temperature reactivity defect is 11612 pcm. In 1129 full-power operating days, the keff will decrease to 0.99126, and the reactor depletes 8.524 × 1026235U atoms. However, the outermost fuel assemblies’ 235U depletion rate is lower than 45% in this extended refuelling cycle, and their ending enrichment is higher than 2.4%. That means the fuel economy of the original design’s two-batch refuelling scheme core layout is insufficient. Improving the thermal neutron fluence in these assemblies may optimize the SMART power performance effectively.
Combining real-time fluorescence spectroscopy and flow cytometry to reveal new insights in DOC and cell characterization of drinking water
Authors: Schuster Jonas , Huber Johanna , Stumme Jakob , Grieb Anissa , Ernst Mathias 
Published: August 2022

Sudden changes in drinking water quality can cause harmful consequences for end users. Thus, real-time monitoring of drinking water quality can allow early warning and provide crucial gains for securing safe water distribution. This study investigated the advantages of simultaneous real-time measuring of flow cytometry and fluorescence spectroscopy. A contamination event was investigated in a laboratory-scale analysis by spiking drinking water samples with organic nutrients. Flow cytometric data were analyzed by creating fingerprints based on differentiation into high and low nucleic acid cells (HNA/LNA). The detailed characterization of these data showed that an increase in HNA cells indicated an increase in the bacterial growth potential even before actual TCC increases. The fluorescence data was decomposed via the PARAFAC method to reveal seven fluorescent components. Three aromatic protein-like components were associated with the microbiological condition of the drinking water cells; namely, Components 4 (λEx = 279 nm, λEm = 351 nm), 6 (λEx = 279 nm, λEm = 332 nm), and 7 (λEx = 276 nm, λEm = 302 nm). Component 6 was identified as a possible organic variable for appropriate monitoring of TCC, whereas Components 4 and 7 were identified as organic compounds representing nutrients for organisms present in drinking water. Overall, combining both methods for real-time monitoring can be a powerful tool to guarantee drinking water quality.
Genetic Characteristics and Variation Spectrum of USH2A-Related Retinitis Pigmentosa and Usher Syndrome
Authors: Li Wei , Jiang Xiao-Sen , Han Dong-Ming , Gao Jia-Yu , Yang Zheng-Tao , Jiang Li , Zhang Qian , Zhang Sheng-Hai , Gao Ya , Wu Ji-Hong , Li Jian-Kang 
Published: August 2022

Purposes: We aimed to characterize the USH2A genotypic spectrum in a Chinese cohort and provide a detailed genetic profile for Chinese patients with USH2A-IRD.Methods: We designed a retrospective study wherein a total of 1,334 patients diagnosed with IRD were included as a study cohort, namely 1,278 RP and 56 USH patients, as well as other types of IEDs patients and healthy family members as a control cohort. The genotype-phenotype correlation of all participants with USH2A variant was evaluated.Results: Etiological mutations in USH2A, the most common cause of RP and USH, were found in 16.34% (n = 218) genetically solved IRD patients, with prevalences of 14.87% (190/1,278) and 50% (28/56). After bioinformatics and QC processing, 768 distinct USH2A variants were detected in all participants, including 136 disease-causing mutations present in 665 alleles, distributed in 5.81% of all participants. Of these 136 mutations, 43 were novel, nine were founder mutations, and two hot spot mutations with allele count ≥10. Furthermore, 38.5% (84/218) of genetically solved USH2A-IRD patients were caused by at least one of both c.2802T&gt;G and c.8559–2 A&gt;G mutations, and 36.9% and 69.6% of the alleles in the RP and USH groups were truncating, respectively.Conclusion: USH2A-related East Asian-specific founder and hot spot mutations were the major causes for Chinese RP and USH patients. Our study systematically delineated the genotype spectrum of USH2A-IRD, enabled accurate genetic diagnosis, and provided East Asian and other ethnicities with baseline data of a Chinese origin, which would better serve genetic counseling and therapeutic targets selection.
In silico prediction of the animal susceptibility and virtual screening of natural compounds against SARS-CoV-2: Molecular dynamics simulation based analysis
Authors: Garg Priyanka , Vanamamalai Venkata Krishna , Jali Itishree , Sharma Shailesh 
Published: August 2022

COVID-19 is an infectious disease caused by the SARS-CoV-2 virus. It has six open reading frames (orf1ab, orf3a, orf6, orf7a, orf8, and orf10), a spike protein, a membrane protein, an envelope small membrane protein, and a nucleocapsid protein, out of which, orf1ab is the largest ORF coding different important non-structural proteins. In this study, an effort was made to evaluate the susceptibility of different animals against SARS-CoV-2 by analyzing the interactions of Spike and ACE2 proteins of the animals and propose a list of potential natural compounds binding to orf1ab of SARS-CoV-2. Here, we analyzed structural interactions between spike proteins of SARS-CoV-2 and the ACE2 receptor of 16 different hosts. A simulation for 50 ns was performed on these complexes. Based on post-simulation analysis, Chelonia mydas was found to have a more stable complex, while Bubalus bubalis, Aquila chrysaetos chrysaetos, Crocodylus porosus, and Loxodonta africana were found to have the least stable complexes with more fluctuations than all other organisms. Apart from that, we performed domain assignment of orf1ab of SARS-CoV-2 and identified 14 distinct domains. Out of these, Domain 3 (DNA/RNA polymerases) was selected as a target, as it showed no similarities with host proteomes and was validated in silico. Then, the top 10 molecules were selected from the virtual screening of ∼1.8 lakh molecules from the ZINC database, based on binding energy, and validated for ADME and toxicological properties. Three molecules were selected and analyzed further. The structural analysis showed that these molecules were residing within the pocket of the receptor. Finally, a simulation for 200 ns was performed on complexes with three selected molecules. Based on post-simulation analysis (RMSD, RMSF, Rg, SASA, and energies), the molecule ZINC000103666966 was found as the most suitable inhibitory compound against Domain 3. As this is an in silico prediction, further experimental studies could unravel the potential of the proposed molecule against SARS-CoV-2.
Stemness analysis in hepatocellular carcinoma identifies an extracellular matrix gene–related signature associated with prognosis and therapy response
Authors: Chen Lei , Zhang Dafang , Zheng Shengmin , Li Xinyu , Gao Pengji 
Published: August 2022

Background: Tumor stemness is the stem-like phenotype of cancer cells, as a hallmark for multiple processes in the development of hepatocellular carcinoma (HCC). However, comprehensive functions of the regulators of tumor cell’s stemness in HCC remain unclear.Methods: Gene expression data and clinical information of HCC samples were downloaded from The Cancer Genome Atlas (TCGA) dataset as the training set, and three validation datasets were derived from Gene Expression Omnibus (GEO) and International Cancer Genome Consortium (ICGC). Patients were dichotomized according to median mRNA expression–based stemness index (mRNAsi) scores, and differentially expressed genes were further screened out. Functional enrichment analysis of these DEGs was performed to identify candidate extracellular matrix (ECM)–related genes in key pathways. A prognostic signature was constructed by applying least absolute shrinkage and selection operator (LASSO) to the candidate ECM genes. The Kaplan–Meier curve and receiver operating characteristic (ROC) curve were used to evaluate the prognostic value of the signature. Correlations between signatures and genomic profiles, tumor immune microenvironment, and treatment response were also explored using multiple bioinformatic methods.Results: A prognostic prediction signature was established based on 10 ECM genes, including TRAPPC4, RSU1, ILK, LAMA1, LAMB1, FLNC, ITGAV, AGRN, ARHGEF6, and LIMS2, which could effectively distinguish patients with different outcomes in the training and validation sets, showing a good prognostic prediction ability. Across different clinicopathological parameter stratifications, the ECMs signature still retains its robust efficacy in discriminating patient with different outcomes. Based on the risk score, vascular invasion, α-fetoprotein (AFP), T stage, and N stage, we further constructed a nomogram (C-index = 0.70; AUCs at 1-, 3-, and 5-year survival = 0.71, 0.75, and 0.78), which is more practical for clinical prognostic risk stratification. The infiltration abundance of macrophages M0, mast cells, and Treg cells was significantly higher in the high-risk group, which also had upregulated levels of immune checkpoints PD-1 and CTLA-4. More importantly, the ECMs signature was able to distinguish patients with superior responses to immunotherapy, transarterial chemoembolization, and sorafenib.Conclusion: In this study, we constructed an ECM signature, which is an independent prognostic biomarker for HCC patients and has a potential guiding role in treatment selection.
Willingness to utilize cervical cancer screening among Ethiopian women aged 30–65 years
Authors: Desta Adugna Alemu , Alemu Fikadu Tolesa , Gudeta Moges Beya , Dirirsa Dejene Edosa , Kebede Andualem Gezahegn 
Published: August 2022

BackgroundCervical cancer screening is a means of detecting cervical cancer early, before it develops, in order to reduce disease mortality and morbidity. When women are screened for cervical cancer between the ages of 30 and 40 years at least one time in their life, their risk of cancer could be decreased by 25–36%. Despite this advantage, cervical cancer screening coverage in Ethiopia is still &lt;2%. As a result, we wanted to see how ready women in the Girar Jarso district, Ethiopia, were to get a cervical cancer test.MethodologyCommunity-based cross-sectional study was done using a stratified cluster sampling technique among 855 women aged 30–65 years in the Girar Jarso district, Ethiopia, from 1 June 2021 to 1 September 2021. A pretested and semi-structured interviewer-administered questionnaire was used to collect the data. EpiData management version 4.6 was used to enter data, which was then exported to SPSS version 23 for analysis. Logistic regression analysis was performed, and variables with ap-value of &lt;0.05 were taken as statistically significant predictors of the willingness to utilize cervical cancer screening.ResultsOf the 855 women, only 315 (46.7%, CI = 43–50.3) women were willing to be checked for cervical cancer, with 181 (21.2%) women having been screened at least one time in their life. Age of 30–39 years [AOR = 2.80 (95% CI: 1.05, 7.48)], urban resident [AOR = 2.12 (95% CI: 1.06, 4.48)], positive attitude [AOR = 1.68 (95% CI: 1.11, 2.53)], wealth status, awareness of cervical cancer, and low perceived barriers were independent predictors of the willingness to utilize cervical cancer screening.Conclusion and recommendationThe willingness to utilize cervical cancer screening services is low in the Girar Jarso district. To improve community awareness and attitude, continued and sustainable advocacy on the value of cervical cancer screening should be offered through mass media and health extension workers.