bluuebunny/crossref_metadata_2025_split huggingface dataset:
Allele variation in the ferrochelatase gene in a Chinese pedigree of erythropoietic protoporphyria
Authors: Li Dongliang , Lin Xiaoqing , Ao Xiulan , Fang Jian , Zhang Zhiqiang , Zheng Zhiyong , YAN Yongqin 
Published: August 2022

Abstract
Background: Erythropoietic protoporphyria (EPP) is characterized by photosensitive skin lesions and various liver injuries. EPP is an inherited disease closely related to single nucleotide polymorphisms (SNPs) in the ferrochelatase gene ( FECH ). We aim to analyze FECH mutations in a Chinese pedigree in order to help clarify the disease’s pattern of inheritance. Results: We genotyped the FECH gene in a male proband with EPP with liver injury and in his family members in Fujian, China. Since the FECH allele (IVS3-48C) has been associated with up to 98% of patients with EPP, we investigated this mutation in 200 unrelated controls in Fujian. The deletion shift mutation c.757_761delAGAAG was detected in the proband and his brother, father, two uncles, and grandmother. The proband, his brother and two uncles, all of whom showed photosensitive lesions, carried both the c.757_761delAGAAG allele and the IVS3-48C allele. We also detected the mutant allele IVS8-61_-62delCT in this pedigree. In the 200 unrelated subjects, 31.47% carried the IVS3-48C allele. In a pedigree of EPP, we detected the deletion shift mutation c.757_761delAGAAG, which is the first report of such a mutation in China . Conclusions: Based on our data, the mode of inheritance requires a haplotype formed by two loci, IVS3-48T>Cand c.757_761delAGAAG.
Mitigating local human impacts promotes coral reef persistence under climate change
Authors: Gove Jamison , Williams Gareth , Lecky Joey , Brown Eric , Conklin Eric , Counsell Chelsie , Davis Gerald , Donovan Mary , Falinski Kim , Kramer Lindsey , Kozar Kelly , Li Ning , Maynard Jeffrey , Mccutcheon Amanda , McKenna Sheila , Neilson Brian , Safaie Aryan , Teague Christopher , Whittier Robert , Asner Gregory 
Published: August 2022

Abstract
Coastal ecosystems are disproportionally inhabited by global human population. Consequently, human impacts originating from land and sea combine with climate-driven disturbances to fundamentally restructure nearshore marine ecosystems. These coincident human stressors are especially acute in the tropics where population centres concentrate along shorelines and marine heatwaves increasingly trigger mass coral bleaching and mortality. However, despite decades of research, we lack consensus as to whether local management supports coral reef resilience under climate change. Here we combined recurring reef surveys with a unique time series of land-sea human impacts over a 20-year period that encompassed an unprecedented marine heatwave in Hawaiʻi. Reefs with high fish biomass and reduced land-based impacts such as wastewater pollution had positive coral trajectories pre-disturbance and experienced minimal coral loss following severe heat stress. Concurrent land-sea management resulted in a 3- to 7-fold increase in the probability of a reef having high reef-builder cover four years post-disturbance. International efforts to conserve 30% of Earth’s ecosystems are gaining momentum, but do not intrinsically link land and ocean conservation targets. Our findings suggest coupled land-sea policy measures are required to realise global conservation goals and provide coral reefs with the best opportunity for persistence in our changing climate.
N6-methyladenosine reader YTHDF3 contributes to the aerobic glycolysis of osteosarcoma through stabilizing PGK1 stability
Authors: Liu Deyin , Li Zhong , Zhang Kun , Jiao Ning , Lu Daigang , Zhou Dawen , Meng Yibin 
Published: August 2022

Abstract
N6-methyladenosine (m6A) modification is pivotal transcripts chemical modification of eukaryotic, which has been identified to play critical roles on tumor metabolic reprogramming. However, the functions of m6A reading protein YTH N6-methyladenosine RNA binding protein 3 (YTHDF3) in osteosarcoma is still unclear. This research planed to investigate the bio-functions and mechanism in osteosarcoma tumorigenesis. Results indicated that YTHDF3 up-regulated in the osteosarcoma tissue samples and cells, and closely correlated to the poor prognosis of osteosarcoma patients. Functionally, gain and loss-of-functional assays illustrated that YTHDF3 promoted the proliferation and aerobic glycolysis of osteosarcoma cells in vitro, and accelerated the tumor growth in vivo. Mechanistically, a m6A-modified PGK1 mRNA functioned as the target of YTHDF3, and YTHDF3 enhanced the PGK1 mRNA stability via m6A-dependent manner. In conclusion, these findings indicated that YTHDF3 functioned as an oncogene in osteosarcoma tumorigenesis through m6A/PGK1 manner, providing a therapeutic strategy for human osteosarcoma.
Healthcare resource utilization and costs among patients with heart failure with preserved, mildly reduced, and reduced ejection fraction in Spain
Authors: Escobar Carlos , Palacios Beatriz , Varela Luis , Gutiérrez Martín , Duong Mai , Chen Hungta , Justo Nahila , Cid-Ruzafa Javier , Hernández Ignacio , Hunt Phillip , Delgado Juan F. 
Published: August 2022

Abstract
Aims
To describe healthcare resource utilization (HCRU) of patients with heart failure with preserved (HFpEF), mildly reduced (HFmrEF), and reduced ejection fraction (HFrEF) in Spain.
Methods
Adults with ≥ 1 HF diagnosis and ≥ 1 year of continuous enrolment before the corresponding index date (1/January/2016) were identified through the BIG-PAC database. Rate per 100 person-years of all-cause and HF-related HCRU during the year after the index date were estimated using bootstrapping with replacement.
Results
21,297 patients were included, of whom 48.5% had HFrEF, 38.6% HFpEF and 4.2% HFmrEF, with the rest being of unknown EF. Mean age was 78.8 ± 11.8 years, 53.0% were men and 83.0% were in NYHA functional class II/III. At index, 67.3% of patients were taking renin angiotensin system inhibitors, 61.2% beta blockers, 23.4% aldosterone antagonists and 5.2% SGLT2 inhibitors. Rates of HF-related outpatient visits and hospitalization were 968.8 and 51.6 per 100 person-years, respectively. Overall, 31.23% of patients were hospitalized, mainly because of HF (87.88% of total hospitalizations); HF hospitalization length 21.06 ± 17.49 days (median 16; 25th, 75th percentile 9–27). HF hospitalizations were the main cost component: inpatient 73.64%, pharmacy 9.67%, outpatient 9.43%, and indirect cost 7.25%. Rates of all-cause and HF-related HCRU and healthcare cost were substantial across all HF subgroups, being higher among HFrEF compared to HFmrEF and HFpEF patients.
Conclusions
HCRU and cost associated with HF are high in Spain, HF hospitalizations being the main determinant. Medication cost represented only a small proportion of total costs, suggesting that an optimization of HF therapy may reduce HF burden.
An Efficient and Intelligent Conference Management System- Desired Services and Features
Authors: Deutschland Aboli H. Patil Livanova , pune Parikshit N. Mahalle VIIT , MCERC Varsha H. Patil , MCERC Swati A. Bhavsar 
Published: August 2022

Abstract
Conferences and Journals serve as a strong platform and media for research work. Organizing a conference is an extensive, difficult and complex process involving many stakeholders. Due to the increasing number of conferences held worldwide and huge submissions at both conferences and journals, quality has become a highly coveted topic. Today number of systems and platforms for organizing conferences with a varying set of services and features are available. Conference organizer has to choose one of the Conference Management System (CMS) for managing the whole event efficiently. This paper presents a set of desired services and features of efficient CMS; and a comparative study of 30 CMS. The key features desired to be included in efficient CMS include-fair reviews, accurate, reliable, fast, online/web-based, fully automatic, secure assuring confidentiality, user-friendly, and scalable system. Though services and features contribute to overall management and performance of the event it is observed that the overall performance of any conference management mainly depends on the peer review process. For fair reviews integration of intelligent reviewer assignment module in the conference management system is need of the day.
Lipin2 ameliorates diabetic encephalopathy via suppressing JNK/ERK-mediated NLRP3 inflammasome overactivation
Authors: Zhang Xiaochen , Huang Shan , Zhuang Ziyun , Han Xiaolin , Yu Shuyan , Hua Mengyu , Liang Zhonghao , Meng Chao , Yin Ling , Zhuang Xianghua , Chen Shihong 
Published: August 2022

Abstract

OBJECTIVES: Diabetic encephalopathy (DE) is a common complication of diabetes in the central nervous system, which can cause cognitive dysfunction in patients. However, its pathophysiological mechanism has not been elucidated, and thus effective prevention and treatment methods are still lacking. Previous studies reported that neuroinflammation involved in the central neuropathy, while lipin2 plays an important role in inflammatory response. Therefore, we aimed to investigate the effects of lipin2 on regulating inflammatory response in the pathogenesis of DE. METHODS: BV2 cells were treated with high glucose and infected with lipin2 overexpression or knockdown virus to observe the cell viability. Then, we constructed a mouse model of DE, and constructed a lipin2 knockdown or overexpression model by injecting lentivirus into the brain with stereotaxis. The expression of lipin2 in inflammatory bodies and related inflammatory factor signaling pathway-related proteins were examined by western blot and quantitative real-time PCR. Morris water maze was used to evaluate the spatial learning and memory of mice.RESULTS: High glucose decreased the expression of lipin2 in BV2 cells, while overexpression of lipin2 in BV2 cells significantly suppressed the inflammatory response and apoptosis induced by high glucose. Meanwhile, the expression of lipin2 was down-regulated in the hippocampus in a DE mice model. Up-regulation of lipin2 in the hippocampus of DE mice inhibited JNK/ERK signaling pathway, reduced NLRP3 inflammasome-mediated inflammatory response, down-regulated IL-1/TNF-α expression, and improved synaptic plasticity and cognitive dysfunction in mice. Conversely, knockdown of lipin2 increased NLRP3 inflammasome overactivation, caused neuronal abnormalities and cognitive impairment in mice. CONCLUSIONS: Lipin2 may play a neuroprotective role in DE by inhibiting JNK/ERK-mediated NLRP3 inflammasome overactivation and subsequent inflammatory responses. It may be a potential therapeutic target for DE therapy.
A Pair-Task Heuristic for Scheduling Tasks in Heterogeneous Multi-Cloud Environment
Authors: Krishnasamy Kamalam Gobichettipalayam , Periasamy Suresh , Periasamy Keerthika , Veerappan Prasanna Moorthy , Thangavel Gunasekaran , Lamba Ravita , Muthusamy Suresh 
Published: August 2022

Abstract
Heterogeneous multi-cloud environments make use of a collection of varied performance rich cloud resources, linked with huge-speed, performs varied applications which are of computational nature. Applications require distinct computational features for processing. Heterogeneous multi-cloud domain well suits to satisfy the computational need of very big diverse nature of collection of tasks. Mapping problem provides an optimal solution in scheduling tasks to distributed heterogeneous clouds is termed NP-complete, which leads to the ultimate establishment of heuristic problem solving technique. Identifying the heuristic which is appropriate and best still exists as a complicated problem. In this paper, to address scheduling collection of ‘n’ tasks in two groups among a set of 'm' clouds, we propose three heuristics PTL (Pair-Task Threshold Limit), PTMax-Min, and PTMin-Max. Firstly to determine the tasks scheduling order, proposed heuristics based on the tasks attributes calculate tasks threshold value. Tasks sorted in descending value of threshold. Group G1 comprises tasks ordered in descending value of threshold. Group G2 comprises remaining tasks ordered in ascending value of threshold. Secondly, tasks form Group 1 are scheduled first based on minimum completion time, and then tasks in Group 2 are scheduled. The proposed heuristicsare compared with existing heuristics, namely MCT, MET, Min-Min using benchmark dataset. Heuristics PTL, PTMax-Min, and PTMin-Max bring out reduced makespan compared to MCT, MET, and Min-min.
Machines Sounding like Older People: Investigating how older adults perceive an emphatic voice assistant in their first interactions
Authors: Yücel Yeliz , Rızvanoğlu Kerem 
Published: August 2022

Abstract
Universal usability is an essential constituent of HCI, but new technologies are not usable by all populations. This deficit highlights the importance of improving the conversational agents' abilities for disadvantaged populations like older adults. Conversational agents hold a great potential to support older adults, which were affected mainly by the Covid-19 pandemic and were the most psychologically damaged population caused by fear and isolation during the outbreak. In this research, we present the results of a mixed study investigating the potential of voice assistants (VA) for older adults. This study aims to identify older adults’ (ranging in age from 65 to 75) perceptions, needs and challenges when interacting with a smart speaker based voice assistant (Google Home) during the first-time user interaction. We aim to explore the effects of VA’s voice characteristics (mature vs young) and the presence of empathic expression (high empathic expression vs low empathic expression) on the social (perceived support and trust) and functional (perceived self-efficacy toward voice assistants) outcomes of 60 (30 male- 30 female) older adults. This study used a voice-based CA prototype through the Wizard of Oz technique and adopted a multi-method approach. Using CASA (Computers are Social Actors) Paradigm and Similarity Attraction Theory as a foundation, our research revealed that expression of high empathy is a powerful motivator for older adults to perceive a VA as a discreet social companion due to their cultural beliefs and biases. However, we found no evidence that the similarity attraction effect works on older adults when the similarity is mediated since a mature voice has no significant impact on perceived support or trust both qualitatively and quantitatively.
Angiotensin II Type I Receptor -168A/G Polymorphism is Associated withIncreased the Risk of Glioma
Authors: TURAN Taylan , ÖZAYDIN Burak , EMMEZ Ömer Hakan , KAYMAZ Ahmet Memduh , GÖNÜL İpek Işık , BOZKURT Melih , GÖNENÇ Aymelek 
Published: August 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Design of a Quantum circuit for 4-Qubit multiplier using IBM Quantum Computer
Authors: Gudivada A Arunkumar , Panigrahy Asisa Kumar 
Published: August 2022

Abstract

Reversible gates have been one of the emerging research areas in these days. The reversible gates have several applications in the fields of low power CMOS, DNA computing, optical, nanotechnology etc. Quantum computing is one of the fastest and upcoming nanotechnologies that can implement the digital logical circuits using reversible logic gates. In this paper, CNOT, TOFFOLI, FREDKIN and PERES reversible logic gates have been implemented on IBM quantum computer. An efficient one-bit full adder is going to be proposed using the reversible gates like CNOT and TOFFOLI. Later, a 4x4 multiplier will be developed using the proposed one-bit full adder. The proposed multiplier is proved to perform faster calculations compared to the earlier multipliers. The simulation of the presented modules will be done on IBM Quantum computer and the respective probabilities can be observed for different inputs.
Too close for comfort? Attitudes of gynecologic oncologists toward caring for dying patients
Authors: Hmaidan Sarah , Goulder Alison , Bos Lia , Shen Megan , Wellman JD , Prescott Lauren , Brown Alaina 
Published: August 2022

Abstract
PurposeTo assess the association between gynecologic oncologists’ stigma and palliative care referrals among advanced cancer patients. MethodsGynecologic oncologists were surveyed using validated measures to assess stigmatizing attitudes toward palliative care, anticipated stigma of palliative care, acceptance of palliative care, and willingness to refer to palliative care. Descriptive statistics were calculated. Analysis was performed using linear regression.Results1200 physicians received the survey and 108 (9%) completed it. Most were female (69.4%) and white (82.4%). Most practiced in academics (64.8%) in urban environments (71.3%). Respondents did not have anticipated stigma surrounding palliative care referral (mean score 1.89, range 1-7, higher score indicating more stigma), were accepting of palliative care (mean score 1.45, range 1-7, higher score indicating less acceptance), and were willing to refer patients to palliative care (mean score 5.75, range 1-7, higher score indicating more willingness to refer). Linear regression demonstrated females had less anticipated stigma surrounding palliative care (B=-0.213, p=0.04) and higher acceptance of palliative care (B=-0.244, p=0.01). Most surveyed derived satisfaction from work with advanced cancer patients (83%). Nineteen percent were depressed by managing advanced cancer patients. One fourth felt emotionally burned out by dealing with too many deaths. ConclusionsMost gynecologic oncologists did not exhibit stigma surrounding palliative care and derive satisfaction from their work. Some gynecologic oncologists experience depression and burnout related to their profession. This close connection with patients as they transition to the end of life may take a toll on providers.
Factors associated with the oral health-related quality of life of patients with temporomandibular disorder after occlusal splint treatment
Authors: Limpuangthip Nareudee , Potewiratnanond Prangtip , Karunanon Varangkana , Buritep Ansaya , Thawai Athitaya 
Published: August 2022

Abstract
Objectives
To determine the oral health-related quality of life (OHRQoL) of temporomandibular disorder (TMD) patients after occlusal splint treatment, and to investigate the associated factors.
Materials and methods
This cross-sectional study comprised 227 TMD patients. The dependent variable was OHRQoL determined by telephone interview using the 14-item Oral Health Impact Profile (OHIP-14) questionnaire. The independent variables were collected from dental record, comprising age, sex, treatment duration, clinical diagnosis, clinical parameters, and pain perception. The TMD patients were diagnosed as having masticatory muscle disorders (TMDM), temporomandibular joint (TMJ) disorders (TMDJ), or combined muscle and TMJ disorders (TMDC). Bivariate analyses were performed and multivariable linear regression was used to analyze the factors associated with the OHIP-14 score.
Results
The bivariate analyses demonstrated higher OHIP-14 scores in being younger, females, having TMDC, and lower mouth opening distance. The multivariable analysis demonstrated the association of higher OHIP-14 scores with being younger and having TMDC. The participants with TMDC demonstrated greater improvement in unassisted mouth opening distance, compared with the other clinical diagnosis groups.
Conclusions
After TMD treatment, oral health impact problems remained, but with low frequency. Better OHRQoL after TMD treatment was found in older and TMDC patients.
Correlation between systolic blood pressure and alanine aminotransferase in children and adolescents with short stature based on growth hormone peak
Authors: Jiang Mingming , Zhang Xinran , Zhao Qianqian , Zhang Mei 
Published: August 2022

Abstract
Objective: This study aimed to investigate the relationship between systolic blood pressure (SBP) and alanine aminotransferase (ALT) in children and adolescents with short stature based on growth hormone peak (peak GH).Methods: In this study, 1151 patients diagnosed with short stature in the Shandong Growth and Developmental Disease Follow-up Study Cohort were included and anthropometric and biochemical parameters were measured. In accordance with the peak GH level in the growth hormone challenge test, all subjects were divided into three groups to determine the correlation between SBP and ALT in children and adolescents with schizophrenia: peak GH < 5 ng/mL, 5 ng/mL ≤ peak GH < 10 ng/mL and peak GH ≥ 10 ng/mL. Cross-sectional analysis of the correlation between SBP and ALT in children and adolescents with short stature.Result: The serum ALT levels in the GH peak <5 ng/mL and 5 ng/mL ≤GH < 10 ng/mL groups were elevated compared with those in the GH peak ≥10 ng/mL group. Univariate analysis showed a significant positive correlation between SBP and ALT in subjects with peak GH <5 ng/mL and 5 ng/mL ≤ peak GH < 10 ng/mL (P < 0.05). After adjusting for possible confounding factors, further smooth curve fitting found a nonlinear relationship between SBP and ALT. Further analysis of threshold effects showed that in the GH peak < 5 ng/mL group, the serum ALT levels increased with SBP when SBP reached 116 mmHg (β 0.35; 95% CI 0.04, 0.67; P = 0.029). In the 5 ng/mL ≤ GH < 10 ng/mL group, the serum ALT levels increased with SBP when SBP reached 106 mmHg (β 0.19; 95% CI 0.05, 0.34; P = 0.010). However, in subjects with peak GH <5 ng/mL and 5 ng/mL ≤ peak GH <10 ng/mL, no correlation was observed when the SBP levels does not reach the inflection point. The relationship between serum ALT levels and SBP was not significant in the GH peak ≥10 ng/mL group (β 0.05; 95% CI −0.02, 0.11; P = 0.140).Conclusion: The serum ALT levels in the GH peak < 5 ng/mL and 5 ng/mL ≤GH peak < 10 ng/mL groups were elevated compared with those in the GH peak ≥ 10 ng/mL group and a nonlinear relationship was found between SBP and ALT. When SBP reached the inflection point, the serum ALT levels were positively correlated with the increase in SBP. Future research is required to explore this relationship and mechanism.
Complete genome sequence of a novel mycovirus from Pleurotus citrinopileatus
Authors: Li Xuefei , Li Shiyu , Yin Weiqi , Sossah Frederick Leo , Song Bing , Li Yu , Bo Zhang 
Published: August 2022

Abstract
The complete genome sequence of a novel single-stranded [+ssRNA] positive-sense (+) RNA mycovirus, designated as "Pleurotus citrinopileatus ourmiavirus 1" (PcOV1), isolated from the Pleurotus citrinopileatus strain CCMJ2141, was determined. The complete genome of PcOV1 is composed of 2,535 nucleotides long. It contains a single open reading frame (ORF), which encodes a protein of 657 amino acids (aa) showing conserved domains of RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis based on the RdRp revealed that PcOV1 is a new member of the genus ourmiavirus in the family Botourmiaviridae. This is the first virus characterized in P. citrinopileatus.
Bilimbi (Averrhoa bilimbi) fruit derived carbon dots for dual sensing of Cu(II) and quinalphos
Authors: Venugopalan P. , Vidya N. 
Published: August 2022

Abstract
Synthesis of highly fluorescent carbon dots from an agro crop through facile one-pot microwave method has been reported. Bilimbi fruit derived carbon dots named as BCDs, exhibited excellent photoluminescent properties and stability. Fluorescence of the system selectively quenched on interaction with Cu(II), due to the complex formation between amine, hydroxyl and carboxyl groups in the surface of the BCDs with Cu(II). The non-fluorescent complex displayed a selective and sensitive turn-on fluorescence behavior on adding organophosphorus pesticide quinalphos. And hence, the prepared system is used for dual sensing purposes with nanomolar level of detection limits. The limit of detection of Cu(II) and quinalphos was estimated as 115 nM and 510 nM, respectively. The entire detection process was visible under UV light of 365 nm. Moreover, the BCDs@Cu(II) nanoprobe was effectively applied as fluorescence sensor of quinalphos in real samples of rice and tea where its presence is frequently reported, with good level of recovery percentages.