bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hermetic SuperGrain bags for controlling storage losses caused by Callosobruchus maculatus Fabricius (Coleoptera: Bruchinae) in stored mung bean (Vigna radiata)
Authors: Yewle Nileshwari Raju , Gupta Suchita V. , Patil Bhagyashree N. , Mann Sandeep , Kandasamy Palani 
Published: July 2022

AbstractMung bean is highly susceptible to insect attack during storage. Hermetic storage is an effective technique to control insect damage. This study investigated the potential of the hermetic SuperGrain bag (SGB) for controlling bruchids during storage. The dry samples were packed in SGB infested with adult bruchids (SGB-I), SGB natural field infested (SGB-N), woven polypropylene bags (WPP-I and WPP-N) and kept at room temperature for 180 days. Oxygen (O2) and carbon dioxide (CO2) concentrations were measured at 15 days intervals. Moisture content, infestation level, seed damage and weight loss were determined at 60 days intervals. Seed colour, hardness, crude protein and fat contents were analysed before and after storage. The O2 level decreased to 10.09%, whereas the CO2 level increased to 8.87% in both SGB-I and SGB-N treatments. The moisture content of mung bean was maintained as onset storage in both SGB-N and SGB-I treatments, whereas reduced in WPP-N (9.26% db) and WPP-I (9.21% db). In SGB treatments, no significant bruchids were detected, but they increased drastically in WPP-N (52 ± 9) and WPP-I (377 ± 14). Seed damage (2–3%) and weight loss (0.8–1.0%) were recorded in both SGB-N and SGB-I. Conversely, seed damage reached 26.67 and 54.17%, corresponding to weight losses of 12.33 and 20.82% in WPP-N and WPP-I, respectively. Seed colour, hardness, crude protein and fat contents in SGBs showed no significant changes than in the WPP bags. The study illustrated that the SGB is an efficient hermetic device in protecting mung beans against bruchids attacks compared to the WPP bags.
Conditions d'une démocratie en santé d'ordre maximaliste
Authors: Gross Olivia 
Published: July 2022

RésuméLes motivations des usagers du système de santé et des acteurs traditionnels à s'engager dans des pratiques participatives diffèrent. Les usagers entendent améliorer l'expérience patient en réduisant les injustices épistémiques et en augmentant les libertés de bien-être, le tout dans une perspective de justice sociale. Six logiques guidant les participations coexistent (utilitariste, méthodologique, démocratique, consumériste, épistémique et émancipatoire), logiques dont la compatibilité n'est pas évidente. Par ailleurs, la démocratie en santé est devenue protéiforme et des tensions apparaissent entre la démocratie représentative et une démocratie participative, de type indirect. Cumuler les profils épistémiques d'usagers et les différentes envies et raisons d'agir, puis les envisager sous le prisme de la complémentarité, pourrait atténuer ces tensions.
A study on the executive functioning skills of Greek–English bilingual children – a nearest neighbour approach
Authors: Papastergiou Athanasia , Sanoudaki Eirini , Tamburelli Marco , Chondrogianni Vasiliki 
Published: July 2022

AbstractFindings of bilingual participants outperforming their monolingual counterparts in executive functioning tasks have been repeatedly reported in the literature (Bialystok, 2017). However, uncontrolled factors or imperfectly matched samples might affect the reliability of these findings. This study aims to take into account a range of relevant variables in combination with innovative analyses to investigate the performance of one unstudied language group, Greek–English bilingual children in the north of England, compared to monolingual control groups. Our battery of executive function tasks taps into inhibition, updating and shifting. We use k-means nearest neighbour methods to match the groups and factor analysis to determine language proficiency. We find that bilinguals’ accuracy is on a par with their monolingual peers – however, they are faster in inhibition and working memory tasks. Our study provides strong evidence for the presence of a bilingual advantage in these domains, while making important methodological contributions to the field.
Anisotropy and Microcrack Propagation Induced by Weathering, Regional Stresses and Topographic Stresses
Authors: Xu Tingting , Shen Xianda , Reed Miles , West Nicole , Ferrier Ken L. , Arson Chloé 
Published: July 2022

AbstractThis paper presents a new model for anisotropic damage in bedrock under the combined influences of biotite weathering, regional stresses, and topographic stresses. We used the homogenization theory to calculate the mechanical properties of a rock representative elementary volume made of a homogeneous matrix, biotite inclusions that expand as they weather, and ellipsoidal cracks of various orientations. With this model, we conducted a series of finite element simulations in bedrock under gently rolling topography with two contrasting spatial patterns in biotite weathering rate and a range of biotite orientations. In all simulations, damage is far more sensitive to biotite weathering than to topographic or regional stresses. The spatial gradient of damage follows that of the imposed biotite weathering rate at all times. The direction of micro‐cracks tends to align with that of the biotite minerals. Relative to the topographic and regional stresses imparted by the boundary conditions of the model, the stress field after 1,000 years of biotite weathering exhibits higher magnitudes, wider shear stress zones at the feet of hills, more tensile vertical stress below the hilltops, and more compressive horizontal stress concentrated in the valleys. These behaviors are similar in simulations of slowing eroding topography and static topography. Over longer periods of time (500 kyr), the combined effects or weathering and erosion result in horizontal tensile stress under the hills and vertical tensile stress under and in the hills. These simulations illustrate how this model can help elucidate the influence of mineral weathering on Critical Zone evolution.
Therapy-related clonal cytopenia as a precursor to therapy-related myeloid neoplasms
Authors: Shah Mithun Vinod , Mangaonkar Abhishek A. , Begna Kebede H. , Alkhateeb Hassan B. , Greipp Patricia , Nanaa Ahmad , Elliott Michelle A. , Hogan William J. , Litzow Mark R. , McCullough Kristen , Tefferi Ayalew , Gangat Naseema , Patnaik Mrinal M. , Al-Kali Aref , He Rong , Chen Dong 
Published: July 2022

AbstractTherapy-related myeloid neoplasms (t-MN) are aggressive leukemia that develops as a complication of prior exposure to DNA-damaging agents. Clonal cytopenia of undetermined significance (CCUS) is a precursor of de novo myeloid neoplasms. Characteristics of CCUS that develop following cytotoxic therapies (therapy-related clonal cytopenia, t-CC) and outcomes following t-CC have not been described. We identified 33 patients with t-CC and compared to a cohort of the WHO-defined t-MN (n = 309). t-CC had a distinct genetic and cytogenetic profile: pathogenic variants (PV) in TET2 and SRSF2 were enriched in t-CC, whereas TP53 PV was more common in t-MN. Ten (30%) t-CC patients developed a subsequent t-MN, with a cumulative incidence of 13%, 23%, and 50% at 6 months, 1, and 5 years, respectively. At t-MN progression, 44% of evaluable patients had identifiable clonal evolution. The median survival following t-CC was significantly superior compared all t-MN phenotype including t-MDS with <5% bone marrow blasts (124.5 vs. 16.3 months, P < 0.001) respectively. The presence of cytogenetic abnormality and the absence of variants in DNMT3A, TET2, or ASXL1 (DTA-genes) were associated with a higher likelihood of developing a subsequent t-MN and an inferior survival. We describe a putative precursor entity of t-MN with distinct features and outcomes.
Interprofessional learning for dental and pharmacy professionals: learning together changes how you work together
Authors: Barraclough Caroline , Patel Jalpa , Grimes Lesley , Shaw Matthew 
Published: July 2022

AbstractCan an interprofessionally designed and facilitated learning event change the way professionals understand each other's roles, enable them to better work with each other and improve patient care? Pharmacy and dental professionals are contractors to the NHS, providing services to the public. The way both professions are funded encourages them to generally work in isolation from the wider NHS, in contrast to other areas of healthcare and NHS systems. This study explores how working collaboratively at all stages of design, development, facilitation and engagement of a learning event impacts on the professionals taking part. It also explores how learning interprofessionally can change the way dental and pharmacy professionals work together, suggesting this way of learning is beneficial to improving working relationships between the sectors.The study explored the ways that shared learning between professions could be approached and encouraged. Pharmacy professionals expressed that they felt more informed and confident giving dental advice to patients. Dental professionals recognised that pharmacy professionals could help support and manage patients. All professionals could see the importance of multidisciplinary working to improve understanding of the other professionals' role. The workshops showed that shared learning is an important aspect to help engage and integrate healthcare systems.
van der Waals integration of GaN light-emitting diode arrays on foreign graphene films using semiconductor/graphene heterostructures
Authors: Singh Anuj Kumar , Ahn Kwangseok , Yoo Dongha , Lee Seokje , Ali Asad , Yi Gyu-Chul , Chung Kunook 
Published: July 2022

AbstractWe report the van der Waals integration of micropatterned GaN light-emitting diodes (LEDs) onto foreign graphene films. GaN micro-LEDs were selectively grown on a graphene substrate using a patterned SiO2 mask, and then the whole device structure was laterally fixed by a polyimide insulator to form a united layer. After device fabrication, the LED/graphene heterostructure device was piled on the foreign graphene layers using a typical wet transfer technique of 2D crystals where the bottom graphene layer of the heterostructure was adhered to the foreign graphene only by van der Waals interactions. The transferred micro-LEDs showed well-aligned crystallographic orientations as well as reliable device performances, including strong light emissions, good rectifying behaviors of the current density–voltage curve, and good simultaneity between the electroluminescence intensity and the applied currents, ensuring reliable electrical connections and mechanical adhesions of the light-emitting layer to the foreign graphene films. Furthermore, the reliable adhesiveness allowed us to achieve device wearability, while the LEDs exhibited homogeneous light emissions under various bending conditions because of negligible external stress in the discrete micro-LEDs.
Megathrust reflectivity reveals the updip limit of the 2014 Iquique earthquake rupture
Authors: Ma Bo , Geersen Jacob , Lange Dietrich , Klaeschen Dirk , Grevemeyer Ingo , Contreras-Reyes Eduardo , Petersen Florian , Riedel Michael , Xia Yueyang , Tréhu Anne M. , Kopp Heidrun 
Published: July 2022

AbstractThe updip limit of seismic rupture during a megathrust earthquake exerts a major control on the size of the resulting tsunami. Offshore Northern Chile, the 2014 Mw 8.1 Iquique earthquake ruptured the plate boundary between 19.5° and 21°S. Rupture terminated under the mid-continental slope and did not propagate updip to the trench. Here, we use state-of-the-art seismic reflection data to investigate the tectonic setting associated with the apparent updip arrest of rupture propagation at 15 km depth during the Iquique earthquake. We document a spatial correspondence between the rupture area and the seismic reflectivity of the plate boundary. North and updip of the rupture area, a coherent, highly reflective plate boundary indicates excess fluid pressure, which may prevent the accumulation of elastic strain. In contrast, the rupture area is characterized by the absence of plate boundary reflectivity, which suggests low fluid pressure that results in stress accumulation and thus controls the extent of earthquake rupture. Generalizing these results, seismic reflection data can provide insights into the physical state of the shallow plate boundary and help to assess the potential for future shallow rupture in the absence of direct measurements of interplate deformation from most outermost forearc slopes.
Effectiveness and protection duration of Covid-19 vaccines and previous infection against any SARS-CoV-2 infection in young adults
Authors: Rennert Lior , Ma Zichen , McMahan Christopher S. , Dean Delphine 
Published: July 2022

AbstractData on effectiveness and protection duration of Covid-19 vaccines and previous infection against general SARS-CoV-2 infection in general populations are limited. Here we evaluate protection from Covid-19 vaccination (primary series) and previous infection in 21,261 university students undergoing repeated surveillance testing between 8/8/2021–12/04/2021, during which B.1.617 (delta) was the dominant SARS-CoV-2 variant. Estimated mRNA-1273, BNT162b2, and AD26.COV2.S effectiveness against any SARS-CoV-2 infection is 75.4% (95% CI: 70.5-79.5), 65.7% (95% CI: 61.1-69.8), and 42.8% (95% CI: 26.1–55.8), respectively. Among previously infected individuals, protection is 72.9% when unvaccinated (95% CI: 66.1–78.4) and increased by 22.1% with full vaccination (95% CI: 15.8–28.7). Statistically significant decline in protection is observed for mRNA-1273 (P < .001), BNT162b2 (P < .001), but not Ad26.CoV2.S (P = 0.40) or previous infection (P = 0.12). mRNA vaccine protection dropped 29.7% (95% CI: 17.9–41.6) six months post- vaccination, from 83.2% to 53.5%. We conclude that the 2-dose mRNA vaccine series initially offers strong protection against general SARS-CoV-2 infection caused by the delta variant in young adults, but protection substantially decreases over time. These findings indicate that vaccinated individuals may still contribute to community spread. While previous SARS-CoV-2 infection consistently provides moderately strong protection against repeat infection from delta, vaccination yields a substantial increase in protection.
dia-PASEF data analysis using FragPipe and DIA-NN for deep proteomics of low sample amounts
Authors: Demichev Vadim , Szyrwiel Lukasz , Yu Fengchao , Teo Guo Ci , Rosenberger George , Niewienda Agathe , Ludwig Daniela , Decker Jens , Kaspar-Schoenefeld Stephanie , Lilley Kathryn S. , Mülleder Michael , Nesvizhskii Alexey I. , Ralser Markus 
Published: July 2022

AbstractThe dia-PASEF technology uses ion mobility separation to reduce signal interferences and increase sensitivity in proteomic experiments. Here we present a two-dimensional peak-picking algorithm and generation of optimized spectral libraries, as well as take advantage of neural network-based processing of dia-PASEF data. Our computational platform boosts proteomic depth by up to 83% compared to previous work, and is specifically beneficial for fast proteomic experiments and those with low sample amounts. It quantifies over 5300 proteins in single injections recorded at 200 samples per day throughput using Evosep One chromatography system on a timsTOF Pro mass spectrometer and almost 9000 proteins in single injections recorded with a 93-min nanoflow gradient on timsTOF Pro 2, from 200 ng of HeLa peptides. A user-friendly implementation is provided through the incorporation of the algorithms in the DIA-NN software and by the FragPipe workflow for spectral library generation.
Cardiac disruption of SDHAF4-mediated mitochondrial complex II assembly promotes dilated cardiomyopathy
Authors: Wang Xueqiang , Zhang Xing , Cao Ke , Zeng Mengqi , Fu Xuyang , Zheng Adi , Zhang Feng , Gao Feng , Zou Xuan , Li Hao , Li Min , Lv Weiqiang , Xu Jie , Long Jiangang , Zang Weijin , Chen Jinghai , Gao Feng , Ding Jian , Liu Jiankang , Feng Zhihui 
Published: July 2022

AbstractSuccinate dehydrogenase, which is known as mitochondrial complex II, has proven to be a fascinating machinery, attracting renewed and increased interest in its involvement in human diseases. Herein, we find that succinate dehydrogenase assembly factor 4 (SDHAF4) is downregulated in cardiac muscle in response to pathological stresses and in diseased hearts from human patients. Cardiac loss of Sdhaf4 suppresses complex II assembly and results in subunit degradation and complex II deficiency in fetal mice. These defects are exacerbated in young adults with globally impaired metabolic capacity and activation of dynamin-related protein 1, which induces excess mitochondrial fission and mitophagy, thereby causing progressive dilated cardiomyopathy and lethal heart failure in animals. Targeting mitochondria via supplementation with fumarate or inhibiting mitochondrial fission improves mitochondrial dynamics, partially restores cardiac function and prolongs the lifespan of mutant mice. Moreover, the addition of fumarate is found to dramatically improve cardiac function in myocardial infarction mice. These findings reveal a vital role for complex II assembly in the development of dilated cardiomyopathy and provide additional insights into therapeutic interventions for heart diseases.
Cryo-EM structures of recombinant human sodium-potassium pump determined in three different states
Authors: Guo Yingying , Zhang Yuanyuan , Yan Renhong , Huang Bangdong , Ye Fangfei , Wu Liushu , Chi Ximin , shi Yi , Zhou Qiang 
Published: July 2022

AbstractSodium-Potassium Pump (Na+/K+-ATPase, NKA) is an ion pump that generates an electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. During each Post-Albers cycle, NKA exchanges three cytoplasmic sodium ions for two extracellular potassium ions through alternating changes between the E1 and E2 states. Hitherto, several steps remained unknown during the complete working cycle of NKA. Here, we report cryo-electron microscopy (cryo-EM) structures of recombinant human NKA (hNKA) in three distinct states at 2.7–3.2 Å resolution, representing the E1·3Na and E1·3Na·ATP states with cytosolic gates open and the basic E2·[2K] state, respectively. This work provides the insights into the cytoplasmic Na+ entrance pathway and the mechanism of cytoplasmic gate closure coupled with ATP hydrolysis, filling crucial gaps in the structural elucidation of the Post-Albers cycle of NKA.
Shallow subsurface heat recycling is a sustainable global space heating alternative
Authors: Benz Susanne A. , Menberg Kathrin , Bayer Peter , Kurylyk Barret L. 
Published: July 2022

AbstractDespite the global interest in green energy alternatives, little attention has focused on the large-scale viability of recycling the ground heat accumulated due to urbanization, industrialization and climate change. Here we show this theoretical heat potential at a multi-continental scale by first leveraging datasets of groundwater temperature and lithology to assess the distribution of subsurface thermal pollution. We then evaluate subsurface heat recycling for three scenarios: a status quo scenario representing present-day accumulated heat, a recycled scenario with ground temperatures returned to background values, and a climate change scenario representing projected warming impacts. Our analyses reveal that over 50% of sites show recyclable underground heat pollution in the status quo, 25% of locations would be feasible for long-term heat recycling for the recycled scenario, and at least 83% for the climate change scenario. Results highlight that subsurface heat recycling warrants consideration in the move to a low-carbon economy in a warmer world.
The cholesterol uptake regulator PCSK9 promotes and is a therapeutic target in APC/KRAS-mutant colorectal cancer
Authors: Wong Chi Chun , Wu Jian-Lin , Ji Fenfen , Kang Wei , Bian Xiqing , Chen Huarong , Chan Lam-Shing , Luk Simson Tsz Yat , Tong Samuel , Xu Jiaying , Zhou Qiming , Liu Dabin , Su Hao , Gou Hongyan , Cheung Alvin Ho-Kwan , To Ka Fai , Cai Zongwei , Shay Jerry W. , Yu Jun 
Published: July 2022

AbstractTherapeutic targeting of KRAS-mutant colorectal cancer (CRC) is an unmet need. Here, we show that Proprotein Convertase Subtilisin/Kexin type 9 (PSCK9) promotes APC/KRAS-mutant CRC and is a therapeutic target. Using CRC patient cohorts, isogenic cell lines and transgenic mice, we identify that de novo cholesterol biosynthesis is induced in APC/KRAS mutant CRC, accompanied by increased geranylgeranyl diphosphate (GGPP)─a metabolite necessary for KRAS activation. PCSK9 is the top up-regulated cholesterol-related gene. PCSK9 depletion represses APC/KRAS-mutant CRC cell growth in vitro and in vivo, whereas PCSK9 overexpression induces oncogenesis. Mechanistically, PCSK9 reduces cholesterol uptake but induces cholesterol de novo biosynthesis and GGPP accumulation. GGPP is a pivotal metabolite downstream of PCSK9 by activating KRAS/MEK/ERK signaling. PCSK9 inhibitors suppress growth of APC/KRAS-mutant CRC cells, organoids and xenografts, especially in combination with simvastatin. PCSK9 overexpression predicts poor survival of APC/KRAS-mutant CRC patients. Together, cholesterol homeostasis regulator PCSK9 promotes APC/KRAS-mutant CRC via GGPP-KRAS/MEK/ERK axis and is a therapeutic target.
A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice
Authors: Tian Xiaodong , Zhang Yiyu , Li Xinyu , Xiong Ying , Wu Tianchen , Ai Hui-Wang 
Published: July 2022

AbstractAlthough fluorescent indicators have been broadly utilized for monitoring bioactivities, fluorescence imaging, when applied to mammals, is limited to superficial targets or requires invasive surgical procedures. Thus, there is emerging interest in developing bioluminescent indicators for noninvasive mammalian imaging. Bioluminescence imaging (BLI) of neuronal activity is highly desired but hindered by insufficient photons needed to digitalize fast brain activities. In this work, we develop a luciferase prosubstrate deliverable at an increased dose and activated in vivo by nonspecific esterase. We further engineer a bright, bioluminescent indicator with robust responsiveness to calcium ions (Ca2+) and appreciable emission above 600 nm. Integration of these advantageous components enables the imaging of the activity of neuronal ensembles in awake mice minimally invasively with excellent signal-to-background and subsecond temporal resolution. This study thus establishes a paradigm for studying brain function in health and disease.
Mimicked synthetic ribosomal protein complex for benchmarking crosslinking mass spectrometry workflows
Authors: Matzinger Manuel , Vasiu Adrian , Madalinski Mathias , Müller Fränze , Stanek Florian , Mechtler Karl 
Published: July 2022

AbstractCross-linking mass spectrometry has matured to a frequently used tool for the investigation of protein structures as well as interactome studies up to a system-wide level. The growing community generated a broad spectrum of applications, linker types, acquisition strategies and specialized data analysis tools, which makes it challenging to decide for an appropriate analysis workflow. Here, we report a large and flexible synthetic peptide library as reliable instrument to benchmark crosslink workflows. Additionally, we provide a tool, IMP-X-FDR, that calculates the real, experimentally validated, FDR, compares results across search engine platforms and analyses crosslink properties in an automated manner. We apply the library with 6 commonly used linker reagents and analyse the data with 6 established search engines. We thereby show that the correct algorithm and search setting choice is highly important to improve identification rate and reliability. We reach identification rates of up to ~70 % of the theoretical maximum (i.e. 700 unique lysine-lysine cross-links) while maintaining a real false-discovery-rate of <3 % at cross-link level with high reproducibility, representatively showing that our test system delivers valuable and statistically solid results.