bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein kinase CK2 modulates the activity of Maf-family bZIP transcription factor NRL in rod photoreceptors of mammalian retina
Authors: Liang Xulong , Yadav Sharda P , Batz Zachary A , Nellissery Jacob , Swaroop Anand 
Published: October 2022

AbstractMaf-family basic motif leucine zipper protein NRL specifies rod photoreceptor cell fate during retinal development and, in concert with homeodomain protein CRX and other regulatory factors, controls the expression of most rod-expressed genes including the visual pigment gene Rhodopsin (Rho). Transcriptional regulatory activity of NRL is modulated by post-translational modifications, especially phosphorylation, and mutations at specific phosphosites can lead to retinal degeneration. During our studies to elucidate NRL-mediated transcriptional regulation, we identified protein kinase CK2 in NRL-enriched complexes bound to Rho promoter-enhancer regions and in NRL-enriched high molecular mass fractions from the bovine retina. The presence of CK2 in NRL complexes was confirmed by co-immunoprecipitation from developing and adult mouse retinal extracts. In vitro kinase assay and bioinformatic analysis indicated phosphorylation of NRL at Ser117 residue by CK2. Co-transfection of Csnk2a1 cDNA encoding murine CK2 with human NRL and CRX reduced the bovine Rho promoter-driven luciferase expression in HEK293 cells and mutagenesis of NRL-Ser117 residue to Ala restored the reporter gene activity. In concordance, overexpression of CK2 in the mouse retina in vivo by electroporation resulted in reduction of Rho promoter-driven DsRed reporter expression as well as the transcript level of many phototransduction genes. Thus, our studies demonstrate that CK2 can phosphorylate Ser117 of NRL. Modulation of NRL activity by CK2 suggests intricate interdependence of transcriptional and signaling pathways in maintaining rod homeostasis.
Targeted approaches to improve tomato fruit taste
Authors: Wang Shouchuang , Qiang Qi , Xiang Lijun , Fernie Alisdair R , Yang Jun 
Published: October 2022

Abstract
Tomato (Solanum lycopersicum) is the most valuable fruit and horticultural crop species worldwide. Compared with the fruits of their progenitors, those of modern tomato cultivars are, however, often described as having unsatisfactory taste or lacking flavor. The flavor of a tomato fruit arises from a complex mix of tastes and volatile metabolites, including sugars, acids, amino acids, and various volatiles. However, considerable differences in fruit flavor occur among tomato varieties, resulting in mixed consumer experiences. While tomato breeding has traditionally been driven by the desire for continual increases in yield and the introduction of traits that provide a long shelf-life, consumers are prepared to pay a reasonable premium for taste. Therefore, it is necessary to characterize preferences of tomato flavor and to define its underlying genetic basis. Here, we review recent conceptual and technological advances that have rendered this more feasible, including multi-omics-based QTL and association analyses, along with the use of trained testing panels, and machine learning approaches. This review proposes how the comprehensive datasets compiled to date could allow a precise rational design of tomato germplasm resources with improved organoleptic quality for the future.
The characteristics of mRNA m6A methylomes in allopolyploid Brassica napus and its diploid progenitors
Authors: Li Zeyu , Li Mengdi , Wu Xiaoming , Wang Jianbo 
Published: October 2022

Abstract
Genome duplication events, comprising whole-genome duplication and single-gene duplication, produce a complex genomic context leading to multiple levels of genetic changes. However, the characteristics of m6A modification, the most widespread internal eukaryotic mRNA modification, in polyploid species are still poorly understood. This study revealed the characteristics of m6A methylomes within the early formation and following the evolution of allopolyploid Brassica napus. We found a complex relationship between m6A modification abundance and gene expression level depending on the degree of enrichment or presence/absence of m6A modification. Overall, the m6A genes had lower gene expression levels than the non-m6A genes. Allopolyploidization may change the expression divergence of duplicated gene pairs with identical m6A patterns and diverged m6A patterns. Compared with duplicated genes, singletons with a higher evolutionary rate exhibited higher m6A modification. Five kinds of duplicated genes exhibited distinct distributions of m6A modifications in transcripts and gene expression level. In particular, tandem duplication-derived genes showed unique m6A modification enrichment around the transcript start site. Active histone modifications (H3K27ac and H3K4me3) but not DNA methylation were enriched around genes of m6A peaks. These findings provide a new understanding of the features of m 6A modification and gene expression regulation in allopolyploid plants with sophisticated genomic architecture.
A tale of two cities: the education and experiences of two ICJ judges in China and America
Authors: Chen Li 
Published: October 2022

Abstract
This article draws upon multi-archival research to rediscover the experiences and research of Judges VK Wellington Koo and Shi Jiuyong at St John’s University in Shanghai and Columbia University in New York City. The former was the first Chinese person to be elected Vice President of the International Court of Justice while the latter became the first Chinese person to helm the Court. It aims to offer crucial insights into the making of these two eminent international judges from China and their perceptions of international law. More importantly, it sheds light on the movement of people—missionaries, law professors and diplomats—and the circulation of their ideas as drivers of the expansion of international law. This article strives to examine these understudied major figures to make the legal history of international dispute settlement more geographically and racially inclusive.
A Matter of the Heart: Why It Is Time to Change How We Talk About Maternal Mortality
Authors: Lord Megan 
Published: October 2022

ABSTRACT
As the global burden of disease shifts from “diseases of poverty” such as diarrhea to “diseases of affluence” like diabetes and heart disease, a parallel shift is underway in maternal health. Maternal death from hemorrhage is decreasing, while deaths resulting from exacerbation of underlying chronic disease are on the rise.
Core orientations and magnetic fields in isolated molecular clouds
Authors: Sharma Ekta , Gopinathan Maheswar , Soam Archana , Lee Chang Won , Seshadri T R 
Published: October 2022

ABSTRACT
Molecular clouds are sites of star formation. Magnetic fields are believed to play an important role in their dynamics and shaping morphology. We aim to study any possible correlation that might exist between the magnetic fields orientation inside the clouds and the magnetic fields at envelope scales and their connection with respect to the observed morphology of the selected clouds. We examine the magnetic field orientation towards the clouds L1512, L1523, L1333, L1521E, L1544, L1517, L1780, and L183, using optical and Planck polarization observations. We also found the correlation between the ambient magnetic field and core orientations derived using Astrodendrogram on the Herschel 250 $\mu$m data. We find that the magnetic fields derived from optical and Planck agree with each other. The derived magnetic fields are aligned along the observed emission of each cloud as seen in Herschel 250 $\mu$m data. We also find that the relative orientation between the cores and the magnetic fields is random. This lack of correlation may arise due to the fact that the core orientation could also be influenced by the different magnetization within individual clouds at higher densities or the feedback effects which may vary from cloud to cloud. The estimated magnetic field strength and the mass-to-flux ratio suggest that all the clouds are in a magnetically critical state except L1333, L1521E, and L183, where the cloud envelope could be strongly supported by the magnetic field lines.
Efficiency of the top-down polycyclic aromatic hydrocarbon-to-fullerene conversion in ultraviolet irradiated environments
Authors: Murga M S , Akimkin V V , Wiebe D S 
Published: October 2022

ABSTRACT
Polycyclic aromatic hydrocarbons (PAHs) and fullerenes play a major role in the physics and chemistry of the interstellar medium (ISM). Based on a number of recent experimental and theoretical investigations we developed a model in which PAHs are subject to photo-dissociation (carbon and hydrogen loss) and hydrogenation. We take into account that dehydrogenated PAHs may fold into closed structures – fullerenes. Fullerenes, in their turn, can be also hydrogenated, becoming fulleranes, and photo-dissociated, losing carbon and hydrogen atoms. The carbon loss leads to shrinking of fullerene cages to smaller ones. We calculate the abundance of PAHs and fullerenes of different sizes and hydrogenation level depending on external conditions: the gas temperature, intensity of radiation field, number density of hydrogen atoms, carbon atoms, and electrons. We highlight the conditions, which are favourable for fullerene formation from PAHs, and we conclude that this mechanism works not only in H-poor environment but also at modest values of hydrogen density up to 104 cm−3. We found that fulleranes can be formed in the ISM, although the fraction of carbon atoms locked in them can be maximum around 10−9. We applied our model to two photo-dissociation regions, Orion Bar and NGC 7023. We compare our estimates of the fullerene abundance and synthetic band intensities in these objects with the observations and conclude that our model gives good results for the closest surroundings of ionizing stars. We also demonstrate that additional fullerene formation channels should operate along with ultraviolet (UV)-induced formation to explain abundance of fullerenes far from UV sources.
Cellular immunotherapy for medulloblastoma
Authors: Schakelaar Michael Y , Monnikhof Matthijs , Crnko Sandra , Pijnappel Emma W , Meeldijk Jan , ten Broeke Toine , Bovenschen Niels 
Published: October 2022

AbstractMedulloblastoma (MB) is the most common malignant brain tumor in children, making up ~20% of all primary pediatric brain tumors. Current therapies consist of maximal surgical resection and aggressive radio- and chemotherapy. A third of the treated patients cannot be cured and survivors are often left with devastating long-term side effects. Novel efficient and targeted treatment is desperately needed for this patient population. Cellular immunotherapy aims to enhance and utilize immune cells to target tumors, and has been proven successful in various cancers. However, for MB, the knowledge and possibilities of cellular immunotherapy are limited. In this review, we provide a comprehensive overview of the current status of cellular immunotherapy for MB, from fundamental in vitro research to in vivo models and (ongoing) clinical trials. In addition, we compare our findings to cellular immunotherapy in glioma, an MB-like intracranial tumor. Finally, future possibilities for MB are discussed to improve efficacy and safety.
Survived and disappeared intra-oceanic arcs of the Paleo-Asian Ocean: evidence from Kazakhstan
Authors: Safonova Inna , Perfilova Alina 
Published: October 2022

ABSTRACTThis paper reviews published and presents new data on U-Pb detrital zircon ages, and petrographic, geochemical and isotope (Sm-Nd, Lu-Hf) compositions obtained from greywacke sandstones of Kazakhstan in order to reconstruct fossil intra-oceanic arcs that once existed at Pacific-type convergent margins of the Paleo-Asian Ocean (PAO) in Paleozoic time. We focus on orogenic belts of central Kazakhstan (Itmurundy and Tekturmas) and eastern Kazakhstan (Zharma and Char) in the western Central Asian Orogenic belt. These orogenic belts host accretionary complexes with greywacke sandstones of early Paleozoic (central Kazakhstan) and middle-late Paleozoic (eastern Kazakhstan) ages. First, we evaluate general perspectives for studying sandstones to reconstruct survived and disappeared magmatic arcs, taking into account episodes of subduction erosion. Then we discuss the analytical data from sandstones to make conclusions about the ages and formation settings of their igneous protoliths and define maximum deposition ages. Finally, we discuss the role of serpentinite mélanges in tectonic reconstructions. We argue that sandstones hosted by accretionary complexes are typically greywackes deposited close to their igneous sources and buried rapidly. The provenances of the studied greywacke sandstones of central and eastern Kazakhstan were dominated by mafic to andesitic igneous protoliths derived from juvenile mantle sources. The igneous rocks in the provenances were emplaced in an intra-oceanic arc setting. The sandstones were deposited in fore-arc/trench basins or, to a lesser degree, in back-arc basins. The data from both sandstones and serpentinite mélanges reconstruct middle-late-Cambrian, Ordovician, late-Devonian and Carboniferous arcs of the western PAO. The middle-late Cambrian arcs were fully destroyed by subduction erosion, whereas the Ordovician and Carboniferous arcs survived. The late-Devonian arcs were also eroded, but partly. Both the early and late Paleozoic active margins of the PAO were characterized by alternating periods of accretionary growth and subduction erosion.
Type 3c
Authors: Vonderau Jennifer Shurney , Desai Chirag S. 
Published: October 2022

ABSTRACT
Type 3c diabetes, also known as pancreatogenic diabetes, occurs when primary pancreatic disorders damage the pancreatic islets of Langerhans. Although often misdiagnosed as type 2 diabetes, type 3c diabetes is different in cause, clinical presentation, treatment, and prognosis. Patients with type 3c diabetes are more likely to experience complications and death related to hypoglycemic events. This article reviews the causes and management of type 3c diabetes, which is estimated to affect 5% to 10% of all patients with diabetes.
Improving Cephalad Lag Screw Placement in the Femoral Head During Cephalomedullary Nailing Using a Novel Augmented Reality System
Authors: Konda Sanjit R. , Solasz Sara , Derken Meghan , Ganta Abhishek , Egol Kenneth A. 
Published: October 2022

Objectives:
To measure the effect of a novel augmented reality software designed to aid in lag screw placement into the femoral head for cephalomedullary nails.

Design:
Retrospective cohort study.

Setting:
Single Level I trauma center.

Patients:
Between November 2017 and December 2020, 114 consecutive patients with a hip fracture that underwent repair with a cephalomedullary nail by one of 2 orthopaedic trauma surgeons were reviewed. Fracture classifications included OTA/AO 31-A1, 31-A2, 31-A3, and 31-B3.

Intervention:
The first 57 patients underwent fracture repair without the software (control) and the subsequent 57 patients underwent repair with use of the augmented reality software (AR).

Main Outcome Measurements:
Tip apex distance (TAD) and femoral head zone (AP: superior, center, inferior; Lateral: anterior, center, posterior) were measured using standardized techniques.

Results:
The mean TAD was lower for the AR versus control cohort (10.7 ± 2.9 mm vs. 15.4 ± 3.8 mm; P < 0.001). TAD <10 mm for AR versus control: 25 (43.9%) versus 3 (5.3%), P < 0.001. TAD <15 mm for AR versus control: 50 (87.7%) versus 44 (77.2%), P < 0.001. On the AP view, center position was achieved in 50.9% versus 7.0% of cases for the AR versus control cohort, respectively. On the lateral view, center position was achieved in 68.4% versus 12.3% of cases for the AR versus control cohort, respectively.

Conclusion:
This study suggests that use of the novel augmented reality software for assistance in lag screw positioning within the femoral head improves overall TAD and ability to achieve the center–center position.

Level of Evidence:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Anxiety, Depression, and Social Skills in Girls with Fragile X Syndrome: Understanding the Cycle to Improve Outcomes
Authors: Lightbody Amy A. , Bartholomay Kristi L. , Jordan Tracy L. , Lee Cindy H. , Miller Jonas G. , Reiss Allan L. 
Published: October 2022

Abstract:

Objective:
Female patients with fragile X syndrome (FXS), a genetic condition associated with a mutation in the FMR1 gene, are at significantly elevated risk for developing anxiety and depression. This study is designed to better understand these symptoms in school-age girls, particularly as they relate to age, social skills, and functional outcomes.

Methods:
We compared 58 girls aged 6 to 16 years with FXS with 46 age-matched, sex-matched, and developmentally matched peers without FXS on measures of anxiety, depression, social skills, adaptive behavior, and quality of life.

Results:
Girls with FXS 10.5 years and older demonstrated significantly higher levels of depression, withdrawal, and social avoidance than girls younger than 10.5 years with FXS (p-values < 0.01). Girls in the comparison group did not show any age-related differences on these measures. The older FXS cohort also showed associations between social communication and interaction skills, adaptive behavior, and measures of anxiety and depression (p-values < 0.05) not seen in the comparison group, regardless of age.

Conclusion:
We found that age seems to play an important role in the development of mood symptoms and that such symptoms are uniquely correlated with social communication and reciprocal social interaction behaviors and adaptive functioning in girls with FXS after puberty. These data suggest a critical window of intervention for girls with FXS in the improvement of social interaction skills and the prevention of social avoidance and symptoms of anxiety and depression, with the ultimate goal of improving quality of life and promoting greater independence.
Association of hormone therapy and changes of objective sleep quality in women of late menopausal transition with sleep disorder: a preliminary study
Authors: Kang Soyeon , Kwon Dong Jin , Hong Jiyeon , Go Minji , Chung Youn-Jee , Kim Mee-Ran , Kim Miji , Cho Hyun Hee , Jeung In Cheul 
Published: October 2022

Abstract

Objective
The aim of this study was to investigate changes in objective sleep quality with hormone therapy (HT) in women with late menopausal transition.

Methods
Healthy midlife women with sleep difficulty who received HT were included. Those undergoing late menopausal transition were screened. Sleep patterns and self-reported questionnaires were collected before and 10 weeks after starting HT.

Results
Ten women who met the criteria (age, 50.1 ± 2.8 years) showed higher sleep efficiency and shorter wakefulness after sleep onset (WASO) 10 weeks after starting HT. However, no significant change was found in objective sleep quality after adjustment for multiple comparisons: sleep efficiency, 84.2 ± 7.7 versus 88.2% ± 4.7%, P = 0.037, adjusted P = 0.259; WASO, 59.0 ± 27.2 minutes versus 41.4 ± 17.4 minutes, P = 0.020, adjusted P = 0.140; average duration per awakening, 2.9 ± 1.0 minutes versus 2.2 ± 0.5 minutes, P = 0.033, adjusted P = 0.231. A better score of subjective sleep quality in the Pittsburgh Sleep Quality Index was observed 10 weeks after starting HT (2.0 ± 0.0 vs 1.2 ± 0.4, P = 0.006, adjusted P = 0.042), but sensitivity analysis did not show consistent results after adjustment for multiple comparisons (2.0 ± 0.0 vs 1.1 ± 0.4, P = 0.020, adjusted P = 0.140). Total scores of the Insomnia Severity Index and Menopause Rating Scale were better 10 weeks after starting HT (Insomnia Severity Index, 14.7 ± 3.0 vs 9.1 ± 3.8, P = 0.010; Menopause Rating Scale, 29.0 ± 5.2 vs 21.6 ± 3.0, P = 0.009) with consistent results in sensitivity analyses. There was no difference in the Epworth Sleepiness Scale before and after HT (7.2 ± 1.7 vs 8.6 ± 4.5, P = 0.309). The change in each objective sleep quality variable before and after HT showed strong positive or negative correlations with the change in only a few items in subjective sleep quality.

Conclusion
Women in the late menopausal transition period showed higher sleep efficiency and shorter WASO after HT; however, multiple comparisons showed no statistically significant difference in objective sleep quality between before and after HT.
Effects of resistance training on C-reactive protein in menopausal and postmenopausal women: a systematic review and meta-analysis of randomized controlled trials
Authors: Loaiza-Betancur Andrés F. , Gómez-Tomás Cinta , Blasco José María , Chulvi-Medrano Iván , Iglesias-González Lisette Ethel 
Published: October 2022

Abstract

Importance
Menopause is a biological stage associated with increased cardiovascular morbidity and mortality due to changes in sex hormone levels.

Objective
This systematic review aimed to investigate the benefits and harms of RT in menopausal and postmenopausal women.

Evidence Review
We searched PubMed, Embase, CENTRAL, Scopus, and Web of Science from inception to 2021, and clinical trial registries. Randomized controlled trials (RCT) in menopausal and postmenopausal women that compared women undergoing RT programs with a control group were included. The primary outcomes were C-reactive protein level and adverse events; and, the secondary outcomes were lipid profile and waist circumference. Two reviewers independently selected the studies, extracted data, and assessed the completeness of RT programs, risk of bias, and quality of evidence using the Grading of Recommendations, Assessment, Development, and Evaluation approach (GRADE). A random-effects model was used. Statistical significance was set at P < 0.05.

Findings
Twelve RCTs published from 2012 to 2020 met the inclusion criteria (n = 482). Four follow-up periods were assessed. RT caused reductions in C-reactive protein levels compared to those in the control group in postmenopausal women in the short- to long-term follow-up (mean difference, −0.47 mg/dL; 95% confidence interval, −0.66 to −0.29; P < 0.00001). Furthermore, RT may reduce C-reactive protein levels even at moderate and moderate-to-high intensity (P < 0.0001 and P = 0.0005, respectively). Similar findings were found for lipid profiles in the short- to long-term follow-up (P < 0.05). RT may have had little to no effect on waist circumference. The certainty of the body of evidence was assessed as very low and downgraded owing to serious study limitations, inconsistency, imprecision, and publication bias.

Conclusions and Relevance
There was very low-quality evidence supporting the benefits of RT compared with control for C-reactive protein levels and lipid profile. No benefits were found for the outcome of waist circumference in postmenopausal women with different comorbidities or risk factors. Safety data were scarce. We have little confidence in the results, and the true effect is likely to be substantially different. Further well-conducted and well-reported RCTs are warranted to strengthen the evidence.

Protocol registration
PROSPERO CRD42020213125.