bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-Time Hybrid Coiled Tubing Service Overcomes the Issue of Pipe Stuck in a Challenging Kuwait Well
Authors: Matar Khaled M. , Al-Bahar Zakaria , Al-Mutairi Abdullah B. , Al-Otaibi Khaled , Al-Azmi Mohammad , Al-Mehanna Mehanna , Alwazzan Abdulatif , Travesso Joshua , Alkreebani Mohammad 
Published: March 2022

Abstract
Many unforeseen circumstances can occur when coiled tubing (CT) is deployed into a well with differential pressure sticking being one such potential problem. Diagnosing wellbore data before operating on a well is important but does not eliminate the possibility of stuck CT. With the increase in use of CT on wells with extreme conditions, the requirement to find solutions that can help retrieve the CT and its bottom hole assembly (BHA) is crucial.
When using conventional CT equipment, the operator will have access to several parameters, such as weight, circulating pressure, wellhead pressure (WHP), and depth. However, all these are surface parameters, meaning they are obtained based on the surface conditions. Having accurate downhole information to accompany the surface data can be much more helpful when encountering differential pressure sticking situations.
This paper presents the application of real-time hybrid CT (RTHCT) technology to overcome stuck CT. The technology incorporates a 4mm hybrid cable injected into a CT string and includes a modular sensing bottom-hole assembly (MSBHA) providing key downhole information. Internal and external pressure and temperature, casing collar locator, gamma-ray, compression, and tension are all examples of the downhole data. A flow-through camera was also added to the BHA to provide 360° visibility in the multilateral well, resulting in a high-value BHA with a total length of approximately 36ft.
The RTHCT technology was initially utilized to stimulate a multi-lateral well composed of 2 legs. The CT became stuck while displacing L1 lateral, which had 6-1/8in internal diameter and 2,647ft lateral length. The reason the CT became stuck was ambiguous. But upon careful evaluation of the wellbore information, it was determined that it was likely due to the differential pressure because L1's lateral open-hole section had a large variance in reservoir pressure (up to 680psi difference).
This paper presents some lessons learned when encountering a stuck CT scenario and the benefits of utilizing the RTHCT technology in challenging well conditions to successfully retrieve the CT. With the
RTHCT technology, the bottom hole pressure (BHP) was determined allowing the downhole sticking point to be estimated. Higher overpull values were then applied along with additional pumping whilst remaining within the operating limits of the BHA.
A Rapid RT-LAMP SARS-CoV-2 Screening Assay for Collapsing Asymptomatic COVID-19 Transmission.
Authors: Allsopp Rebecca , Cowley Caroline , Barber Ruth , Jones Carolyn , Holmes Christopher , Bird Paul , Gohil Shailesh , Blackmore Claire , Tobin Martin , Brunskill Nigel , Baker Philip , Shaw Jacqui 
Published: March 2022

Abstract
Purpose: Todemonstrate the diagnostic performance of rapid SARS-CoV-2 RT-LAMP assays, comparing the performance of genomic versus sub-genomic sequence target with subsequent application in an asymptomatic screening population. Methods: RT-LAMP diagnostic sensitivity (DSe) and specificity (DSp) was determined using 114 RT-PCR clinically positive and 88 RT-PCR clinically negative swab samples processed through the diagnostic RT-PCR service within the University Hospitals of Leicester NHS Trust. A swab-based RT-LAMP SARS-CoV-2 screening programme was subsequently made available to all staff and students at the University of Leicester (Autumn 2020), implemented to ISO 15189:2012 standardsusing NHS IT infrastructure and supported by University Hospital Leicester via confirmatory NHS diagnostic laboratory testing of RT-LAMP ‘positive’ samples. Results: Validation samples reporting a Ct < 20 were detected at 100% DSe and DSp, reducing to 95% DSe (100% DSp) for all samples reporting a Ct < 30 (both genomicdual sub-genomic assays). Advisory screening identified nine positive cases in 1680 symptom free individuals (equivalent to 540 cases per 100,000) with results reported back to participants and feed into national statisticswithin 48 hours.Conclusion: This work demonstrates the utility of a rapid RT-LAMP assay for collapsing transmission of SARS-CoV-2 in an asymptomatic screening population.
Body Mass Index Is Associated with Proteinuria in Stage 2-3a but not Stage 3b-4 CKD Patients: An Observational Cross-sectional Study
Authors: Shen Jianxiao , He Yipei , He Liqun , Li Yuting , Chen Xiaonong , Xu Yaowen , Deng Yueyi , Chen Yi , Zhuang Shougang , Gu Hongwei , Liu Na , Qi Yinghui , Qiao Cheng , Ding Feng , Wu Shengbin , Peng Ai , Cheng Jiafen , Lin Qisheng , Li Shu , Zhang Zhen , Wu Jingkui , Qi Chaojun , Jin Haijiao , Wang Wanpeng , Shao Xinghua , Jiang Na , Wang Qin , Che Xiajing , Mou Shan , Gu Leyi , Ni Zhaohui 
Published: March 2022

Abstract
Background: To investigate the relationship between body mass index (BMI) and proteinuria in stage 2-4 chronic kidney disease (CKD) patients. Methods: This study conducted a multicenter, cross-sectional study of 804 stage 2-4 CKD patients. Multivariate regression analysis, One-way ANOVA and subsequent multiple comparisons analysis were used to explore the relationship between BMI and proteinuria in pre-dialysis CKD patients. Results: Among stage 2-4 CKD patients, those with excessive proteinuria accounted for 85.32% of the total. BMI was an independent risk factor for the presence of proteinuria and positively correlated with the volume of proteinuria. Subgroup studies suggested that in stage 2-3a CKD patients, BMI was closely related to the presence and volume of proteinuria. However, in stage 3b-4 CKD patients, there was non-association between BMI and proteinuria. Furthermore, in stage 2-3a but not stage 3b-4 CKD patients, those with obesity (BMI≥ 28 kg/m2) had higher levels of proteinuria, compared with those with normal weight (BMI< 24 kg/m2) and overweight (24 kg/m2≤ BMI < 28 kg/m2). Conclusion: BMI is associated with the presence and volume of proteinuria in patients with early stage (stage 2-3a) CKD but not late stage (stage 3b-4) CKD. This result suggests that only in early stage CKD patients, weight control is beneficial for reducing proteinuria.Trial registration: ChiCTR-INR-17014069, Registered December 21, 2017.
N-doped graphitic carbon&amp;Co3O4: The efficient catalyst for degradation of Rhodamine B and 2,4,6-Trichlorophenol in Peroxymonosulfate (PMS) system
Authors: AKÇAY HAKKI TÜRKER , DEMİR ADEM , ÖZÇİFÇİ ZEHRA , YUMAK TUĞRUL , Keleş Turgut 
Published: March 2022

Abstract
One of the most important issues in the world in recent years is water pollution, which is brought about by rapid industrialization. Peroxymonosulfate (PMS) oxidation is an effective technique for wastewater treatment. Transition metals such as cobalt, manganese, iron are used to activate PMS. Therefore, in this study, a novel activated carbon and cobalt phthalocyanine catalyst (Co-AC) was synthesized by the solvothermal method.Rhodamine B (RhB) and 2,4,6-Trichlorophenol (2,4,6-TCP) were used as model pollutants in the catalytic tests. The effective catalytic activity was observed at a low concentration of Co-AC and PMS compared to the literature. XRD, TEM, Raman, SEM and XPS techniques were used for the characterization of the as-synthesized catalyst. In addition, the surface area of Co-AC was calculated by BET and QS-DFT analysis. As a result, the degradation of RhB was found to be 97.07% after 16 minutes, and 100% for 2,4,6-TCP after 6 minutes. Experimental parameters such as temperature, pH, the concentration of the catalyst and PMS were studied and optimum conditions were determined In addition, TOC removal values ​​were found to be 46% for RhB and 66% for 2,4,6-TCP, respectively. The ICP-OES technique was used for the cobalt leaching and the results were found to be 1.35 mgL−1 for the RhB solution and 1.38 mgL−1 for 2,4,6-TCP. These results ​​are in good agreement with previously published works. The synthesized activated carbon-supported cobalt-based catalyst in line with these results acts as an effective catalyst especially in the treatment of wastewater containing pollutants such as RhB and 2,4,6-TCP.
TFP5, a Peptide Derived From Cdk5 Activator p35, Protects Pancreatic β-Cells From Glucose Toxicity
Authors: Liu Shunyao , Cao Shilu , Luo Hongyan , Zhang Xia , Bao Li , E Jing , Li Bo , Lan Xiaomei , Zhang Guoqing , Bao Xi , Pant Harish , Zheng Yali 
Published: March 2022

Abstract
Type 2 diabetes mellitus (T2DM) is among the most important public health challenges. The pathological hyperactivity of multifunctional cyclin-dependent kinase 5 (Cdk5) contributes to the pathogenesis of T2DM. P5, a peptide derived from the Cdk5 activator p35, shows potential as a Cdk5 activity inhibitor. However, its inhibitory effect and functional regulation of Cdk5 activity need to be confirmed. In this study, we conjugated P5 with a Fluorescein Isothiocyanate (FITC) tag at the N-terminus and a TAT protein transduction domain containing an 11 amino acid peptide at the C-terminus to synthesize TFP5, which we used to inhibit Cdk5 activity. We then evaluated the efficiency of TFP5 in treating T2DM. We demonstrated that TFP5 effectively penetrated pancreatic β-cells, inhibited the pathological hyperactivity of Cdk5, enhanced insulin secretion, and protected MIN6 cells from apoptosis in pancreatic tissues of db/db mice (Type II diabetes mice). Furthermore, we found that TFP5 reduced inflammation in pancreatic islets by reducing the expression of inflammatory cytokines (including TGF-β, TNF-α, and IL-1β). These data indicate that the TFP5 peptide is a promising candidate for T2DM treatment.
Degradation of carbamazepine by high-voltage direct current gas-liquid plasma with the addition of H2O2 and Fe2+
Authors: Yu Jinming , Yan Weiwen , Zhu Bin , Xu Zimu , Hu Shuheng , Xi Wenhao , Lan Yan , Han Wei , Cheng Cheng 
Published: March 2022

Abstract
Carbamazepine (CBZ) is a typical psychotropic pharmaceutical which is one of the most commonly detected persistent pharmaceuticals in the environment. The degradation of CBZ in the aqueous solution was studied by a direct current (DC) gas-liquid phase discharge plasma combined with different catalysts (H2O2 or Fe2+) in this study. The concentrations of reactive species (H2O2, O3, and NO3− ) and •OH radical yield in the liquid were measured during the discharge process. The various parameters that affect the degradation of CBZ, such as discharge powers, initial concentrations, initial pH values, and addition of catalysts, were investigated. The results indicated that a discharge power at 35.7W achieved the optimal balance on the degradation effect and energy efficiency, and both acidic and alkaline solution conditions were conducive to promoting the degradation of CBZ. Both H2O2 and Fe2+ at low concentration (10–100mg/L of Fe2+, 0.05–2.0 mmol/L of H2O2) were observed contributing to the improvement of the CBZ degradation rate, while the promotional effect of CBZ degradation was weakened even inhibition would occur at high concentrations (100–200 mg/L of Fe2+, 2.0–5.0 mmol/L of H2O2). Furthermore, the total organic carbon (TOC) removal efficiency of CBZ was up to 67.1% in the plasma/Fe2+ (100mg/L) system at 48 min, which suggested a high mineralization efficiency on CBZ could be achieved by employing Fe2+ as a catalyst. Based on the intermediate products identified by UPLC–MS, the possible degradation pathways were proposed. Finally, the growth inhibition assay with E. coli showed that the toxicity of plasma/Fe2+-treated CBZ solution decreased and a relatively low solution toxicity could be achieved. Thus, the plasma/catalyst could be an effective technology for the degradation of pharmaceuticals in aqueous solutions.
Facile Synthesis of Magnetic Znfe2o4/AC Composite to Activate Peroxydisulfate for Dye Degradation Under Visible Light Irradiation
Authors: Song Tingting , He Quanbao , Meng Xiaoyan , He Zhangxing , Ge Ming 
Published: March 2022

Abstract
A facile hydrothermal method was employed to synthesize magnetic ZnFe2O4/active carbon (AC) composite, which was firstly used to activate peroxydisulfate (PDS) for rhodamine B (RhB) degradation under visible LED light irradiation. The ZnFe2O4/AC(4/1)/PDS system can enhance the RhB degradation efficiency by 32.1% compared with ZnFe2O4/PDS system due to the synergistic effect between ZnFe2O4 and AC. The influence of operational parameters (catalyst dosage, PDS concentration, temperature, solution pH and coexisting inorganic anions) on RhB degradation was studied, and the complete degradation of RhB was achieved after 80 min of reaction at operational condition: 0.3 g/L of ZnFe2O4/AC(4/1) and 1.5 g/L of PDS, solution pH of 2.74, reaction temperature of 25°C and initial RhB concentration of 20 mg/L. The quenching experiments, EPR test and XPS analysis were employed to reveal the proposed mechanism, which demonstrated that 1O2 played a more important role than other reactive species (SO4•‒, •OH, O2•−, and h+) in the degradation of RhB. The generation of 1O2 via the two routes was as follows: (i) the in-situ formed active oxygen (O*) reacted with HSO5− to produce 1O2; (ii) O2•− was oxidized by h+ to form 1O2. After five consecutive cycles, the photo-degradation efficiency of RhB by ZnFe2O4/AC(4/1) catalyst slightly decreased from 84.6–79.6%, indicating the excellent reusability of ZnFe2O4/AC(4/1) in oxidation system. Under visible LED light irradiation, the performance of ZnFe2O4/AC/PDS oxidation system for the removal of RhB from the different real water matrices was also studied.
The value of CT radiomics in differentiating mycoplasma pneumoniae pneumonia from streptococcus pneumoniae pneumonia with similar consolidation in children
Authors: Wang Dongdong , Zhao Jianshe , Zhang Ran , Yan Qinghu , Zhou Lu , Han Xiaoyu , Qi Yafei , YU Dexin 
Published: March 2022

Abstract
Objective To investigate the value of CT radiomics in the differentiation of mycoplasma pneumoniae pneumonia (MPP) from streptococcus pneumoniae pneumonia (SPP) with similar CT manifestations in children. Methods 63 children with MPP (n=30) or SPP (n=33) with similar consolidation and surrounding halo on CT images in Qilu Hospital and Qilu Children's Hospital between January 2017 and July 2019 were enrolled in the retrospective study. Radiomic features of the both lesions on plain CT images were extracted including the consolidation part of the pneumonia or both consolidation and surrounding halo area which were respectively delineated at region of interest (ROI) areas on the maximum axial image. The training set (n=43) and the validation set (n=20) were established by stratified random sampling at a ratio of 7:3. By means of variance threshold, the effective radiomics features, Select Best and least absolute shrinkage and selection operator (LASSO) regression method were employed for feature selection. Six classifiers, including k-nearest neighbor(KNN), support vector machine (SVM),extreme gradient boosting(XGBoost), random forest(RF), logistic regression(LR), and decision tree(DT) were used to construct the models based on radiomic features. The diagnostic performance of these models was estimated and compared using the area under the receiver operating characteristic (ROC) curve (AUC), and the accuracy (score) matrix was established to compare and evaluate the results of different radiomics models. Results XGBoost outperformed other classifiers and was selected as the backbone in the classifier with the consolidation + the surrounding halo was taken as ROI to differentiate MPP from SPP in validation set. The AUC value of MPP in validation set was 0.889, the sensitivity and specificity were 0.89 and 0.82, respectively; and the AUC value of SPP validation set was 0.889, the sensitivity and specificity were 0.82 and 0.89, respectively. Conclusion The XGBoost model has the best classification efficiency in the identification of MPP from SPP in children, and the ROI with both consolidation and surrounding halo is most suitable for the delineation.
Performance of Advanced Oxidation Processes in Estrogen Removal: a Systematic Review
Authors: Cadena Estefanía , Díaz Jorge , Marriaga-Cabrales Nilson , Ramirez-Malule Howard 
Published: March 2022

Abstract
The presence of estrogens such as estrone (E1), 17β-estradiol (E2), estriol (E3) and 17α-ethinylestradiol (EE2) in the aquatic environments, even in low concentrations, is an ambient concern that needs an appropriate degradation process in wastewater treatment plants. In this study, a systematic review was carried out to identify the most suitable advanced oxidation processes to remove E1, E2, E3 and EE2 from aqueous effluents. A performance parameter (γ) relating electrical consumption, treated volume and, both initial and final estrogen concentrations was proposed. The initial concentration of estrogen, the kind of water (residual or synthetic) and the pH of the system were the more relevant variables to remove those estrogens. Ozonation was the best treatment for estrogen removal due to the lower γ values as 2.95 102, 6.91 103 and 2.38 103 kWh g−1 for E1, E2 and EE2, respectively in wastewater and 9.40 10−2 kWh g−1 (E1) and 8.50 10−2 kWh g−1 (EE2) in synthetic water, all of them with removal higher than 99%. The operational cost estimated of ozonation was USD$ 23.1/1000 m3 for E1 and E2 treatment in wastewater.
Morphine-induced changes in the function of microglia and macrophages after spinal cord injury
Authors: Terminel Mabel N. , Bassil Carla , Rau Josephina , Trevino Amanda , Ruiz Cristina , Alaniz Robert , Hook Michelle A. 
Published: March 2022

Abstract
Background
Opioids are among the most effective and commonly prescribed analgesics for the treatment of acute pain after spinal cord injury (SCI). However, morphine administration in the early phase of SCI undermines locomotor recovery, increases cell death, and decreases overall health in a rodent contusion model. Based on our previous studies we hypothesize that morphine acts on classic opioid receptors to alter the immune response. Indeed, we found that a single dose of intrathecal morphine increases the expression of activated microglia and macrophages at the injury site. Whether similar effects of morphine would be seen with repeated intravenous administration, more closely simulating clinical treatment, is not known.
Methods
To address this, we used flow cytometry to examine changes in the temporal expression of microglia and macrophages after SCI and intravenous morphine. Next, we explored whether morphine changed the function of these cells through the engagement of cell-signaling pathways linked to neurotoxicity using western blot analysis.
Results
Our flow cytometry studies showed that 3 consecutive days of morphine administration after an SCI significantly increased the number of microglia and macrophages around the lesion. Using western blot analysis, we also found that repeated administration of morphine increases b-arrestin, ERK-1 and dynorphin (an endogenous kappa opioid receptor agonist) production by microglia and macrophages.
Conclusions
These results suggest that morphine administered immediately after an SCI changes the innate immune response by increasing the number of immune cells and altering neuropeptide synthesis by these cells.
Genome-wide association study of aromatase inhibitor discontinuation due to musculoskeletal symptoms
Authors: Hertz Daniel L , Douglas Julie A , Miller Robert M , Kidwell Kelley M , Gersch Christina L , Desta Zeruesenay , Storniolo Anna Maria , Stearns Vered , Skaar Todd C , Hayes Daniel F , Henry N. Lynn , Rae James M 
Published: March 2022

Abstract
Objective
Aromatase inhibitors (AI) are commonly used to treat hormone receptor positive (HR+) breast cancer. AI-induced musculoskeletal syndrome (AIMSS) is a common toxicity that causes AI treatment discontinuation. The objective of this genome-wide association study (GWAS) was to identify genetic variants associated with discontinuation of AI therapy due to AIMSS and attempt to replicate previously reported associations.
Methods
In the Exemestane and Letrozole Pharmacogenetics (ELPh) study, postmenopausal patients with HR+ non-metastatic breast cancer were randomized to letrozole or exemestane. Genome-wide genotyping of germline DNA was conducted followed by imputation. Each imputed variant was tested for association with time-to-treatment discontinuation due to AIMSS using a Cox proportional hazards model assuming additive genetic effects and adjusting for age, baseline pain score, prior taxane treatment, and AI arm. Secondary analyses were conducted within each AI arm and analyses of candidate variants previously reported to be associated with AIMSS risk.
Results
Four hundred ELPh participants were included in the combined analysis. Two variants surpassed the genome-wide significance level in the primary analysis (p-value<5x10−8), an intronic variant (rs79048288) within CCDC148 (HR=4.42, 95% CI: 2.67-7.33) and an intergenic variant (rs912571) upstream of PPP1R14C (HR=0.30, 95% CI: 0.20-0.47). In the secondary analysis, rs74418677, which is known to be associated with expression of SUPT20H, was significantly associated with discontinuation of letrozole therapy due to AIMSS (HR=5.91, 95% CI: 3.16-11.06). We were able to replicate associations for candidate variants previously reported to be associated with AIMSS in this cohort, but were not able to replicate associations for any other variants previously reported in other patient cohorts.
Conclusions
Our GWAS findings identify several candidate variants that may be associated with AIMSS risk from AI generally or letrozole specifically. Validation of these associations in independent cohorts is needed before translating these findings into clinical practice to improve treatment outcomes in patients with HR+ breast cancer.
Assessment of Environmental Impact of Aquaculture Ponds in the Western Delta Region of Andhra Pradesh, India
Authors: Nagaraju Thotakura Vamsi , Malegole Sunil , Chaudhary Babloo 
Published: March 2022

Abstract
Aquaculture is persistent and well established in the delta region of Andhra Pradesh. In recent years, the expansion of aquaculture can confer positive economic growth in the newly formed state. However, the enormous expansion of aqua ponds increases the effluents from aquaculture contains a variety of chemical compounds that could cause negative impacts when released into the environment. This paper presents the effect of un-engineered aquaculture on the environment in the delta region of Andhra Pradesh. The rate of expansion of aquaculture practice in the delta region has been done by using remote sensing and geospatial information system. An experimental investigation was carried out for both soil and water samples collected in the aquaculture ponds, to evaluate the water quality parameters, soil characterization, and soil-contaminant interaction. Based on the geospatial data and field survey, the aquaculture practice in the delta region of Andhra Pradesh was intensive and extending towards the northeast from the southwest. In between 2016 and 2020, aquaculture practice was significantly increased by 6.08%. Moreover, water quality parameters and pond bottom soil show a higher concentration of the ammonia and nitrates. Further, aquaculture leachate may interact with the subsoil and have a negative impact on soil mineralogy and hydraulic conductivity. Extensive experimental data and field surveys revealed that adequate guidelines should be needed to control the pollution load on the ecosystem.