bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification of MATE family, and functional analysis of MATE involved in anthocyanin accumulation in Daucus carota
Authors: Saad Kirti R. , Kumar Gyanendra , Puthusseri Bijesh , Srini Sudhanva M. , Giridhar Parvatam , Shetty Nandini Prasad 
Published: July 2022

Abstract
Multidrug and toxic compound extrusion (MATE) is a family of membrane transporters that transport ions and secondary metabolites like anthocyanins in plants. Although various research on MATE has been carried out on different plant species, this is the first comprehensive report to exploit the Daucus carota genome to identify the MATE gene family. We investigated the chromosomal distribution, gene duplication, motif identification, cis-element analysis, in vitro cultures, stress treatment, protein docking, and simulation of DcMATEs. Our studies identified 45 DcMATEs through genome-wide analysis and detected five segmental and six tandem duplications from its genome. The chromosome distribution, phylogenetic analysis, and cis-regulatory elements revealed the structural diversity and numerous functions associated with the DcMATEs. Further, we quantified anthocyanin content and identified the co-expression of DcMATE21 with anthocyanin biosynthesis genes in in vitro cultures under ABA, SA, MJ, SNP, and PHE stress treatments. In addition, membrane dynamics of DcMATE21 with anthocyanin (cyanidin-3-glucoside) identified the binding pocket showing extensive H-bond interactions with ten amino acids present in the transmembrane helix of 7, 8, and 10 of DcMATE21. This investigation speculates the involvement of DcMATE21 in anthocyanin accumulation in in vitro cultures of D. carota.
A sutible model for drying kenitic of wood fiber using halogen moistuer analayzer
Authors: Arabi Mohammad , Ghalehno Mohammad Dahmardeh 
Published: July 2022

Abstract
Drying wood fiber is a complex, dynamic, highly nonlinear, and energy-intensive process involving simultaneous heat and mass transfer. So, the theoretical modeling of wood fiber drying because wood fiber shrinks anisotropically during a drying process is very complicated. The semi-theoretical and empirical thin-layer drying models developed on a laboratory scale are a suitable option for examining the drying kinetics of lignocellulose materials. In this study, drying kinetics of wood fibers were evaluated using 18 semi-empirical models at three temperatures of 105 °C, 120 °C, and 135 °C, utilizing a halogen moisture analyzer. The findings revealed that raising the temperature increased the drying constant while decreasing the drying time of wood fibers. According to drying curves, no constant drying rate was observed and all the drying process occurred in two falling drying rate periods. More specifically, despite the high initial moisture content of wood fiber about 170% (based on dry weight), the wood fiber drying process was mainly controlled by diffusion mechanism. The fitness of drying curves on semi-theoretical and empirical models based on statistical parameters, including root mean square error (RMSE), sum of square errors (SSE), and coefficient of determination (R2) showed that the Midilli et al. model had the highest coefficient of determination and the lowest error percentage. Also, the moisture content was more accurately predicted by ANN model than the Midilli et al. model.
Evaluation of Plasmid incidence in Bacterial and its Genetic elements in Heavy Metals Tolerant-Antibiotics Resistant Microbes from Petroleum Hydrocarbon Polluted sites
Authors: Peter Antai Sylvester , Bessong Agbor Reagan , Dickson Iwatt Godwin , Emmanuel Ubi Stanley 
Published: July 2022

Abstract
This present study was on evaluation of plasmid incidence in bacterial and its genetic elements in heavy metals tolerant-antibiotics resistant microbes from petroleum hydrocarbon polluted sites. Plasmid incidence among multiple heavy metal tolerant bacterial species from hydrocarbons polluted sites were higher than those from the pristine site but not statistically significant. Class 1 integron incidence was significantly higher in heavy metal tolerant bacterial isolates from polluted ecosystems than those from pristine ecosystems. Plasmid replicon typing of bacteria with multiple heavy metal tolerance and antibiotics resistance index revealed IncN plasmid replicon type carrying class 1 integron, encoding resistance to sulphamethazole/trimethoprim, ampicillin, and tolerance to Cd, Ni, and Cu in Klebsiella pneumoniae isolate from petroleum polluted soil. This is the first report of IncN plasmid in environmental bacteria in Nigeria, particularly from petroleum polluted environment. Conjugation experiment confirmed the possible transferability of antibiotic resistance determinants among isolates in polluted ecosystems. This study shows that petroleum hydrocarbon pollution vis-a-vis heavy metal selective pressure with the abundance of mobile genetic elements amongst isolates from polluted ecosystems could contribute to the dispersing of antibiotic resistance genes, thus posing a serious public health concern.
Prevalence and Factors Associated with Burnout among Nurses in Jeddah: A Single-Institution Cross- sectional Study
Authors: Qedair Jumanah T. , Balubaid Renad , Almadani Raghad , Ezzi Suzana , Qumosani Tarteel , Zahid Rania , Alfayea Turki 
Published: July 2022

Abstract
Background: Health care workers, especially nurses, experience significantly elevated levels of emotional and social stressors in the work environment. Therefore, nurses develop high levels of burnout as the stress persists for prolonged periods. The main purpose of this paper is to measure burnout levels amongst nurses and find a relation between burnout levels and demographic factors.Methods: This descriptive cross-sectional study was held from the 23rd of May till the 6th of September 2021 in King Abdulaziz Medical City of National Guard Health Affairs (KAMC-JD) in Jeddah. Data had been collected voluntarily from the nurses through electronic surveys that included demographic data and the Maslach Burnout Inventory (MBI) that measures three dimensions of burnout which are emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA). The association between demographic variables and burnout had been studied using the Fisher Exact test and binary logistic regression.Results: Out of the 1300 nurses working in KAMC-JD, 250 completed the survey. Burnout had been detected in 112 nurses (44.8%). Out of the 250 nurses, 26.4% were found to be at a high risk of burnout, which means they have high or moderate scores of EE and DP, with low ones in PA. The majority of the participants had high burnout levels in each of burnout components, and 99.6% of them scored high in at least one of the three dimensions. Level of burnout was significantly correlated to certain demographic factors which were the nationality (p-value= 0.01) and working unit (p-value= 0.02). On the other hand, there was no significant association between burnout and age, gender, or marital status.Conclusion: This study proves that a high percentage of nurses fell victims to burnout with a strong association between certain demographic data namely nationality as well as working unit and burnout levels. Taking into consideration the negative impact of burnout on both nurses and patients, conducting more studies about burnout among nurses, its effect on them, and the risk factors behind it is needed. Early treatment and management are also recommended to avoid the undesirable outcomes.
Modified Model Predictive Control based Adaptive Steering Strategy with Nonlinear Compensation by Using Double Deep Q-learning Network Algorithm
Authors: Lin Xinyou , Chen Qianlian , Zhou Binhao , Peng Yuhui 
Published: July 2022

Abstract
Steering control for autonomous vehicles is used for more complex scenarios, such as nonlinear scenarios and varied vehicle speeds scenarios during the actual driving process. Model Predictive Control (MPC) is known as a feasible method for multi-constraints. However, a complicated mathematical model will lead to a great computational burden. To deal with this issue, a modified MPC-based adaptive steering strategy with nonlinear compensation by using Double Deep Q-learning Network Algorithm (DDQN) is proposed. Considering the real-time requirement for steering control, MPC is used as a basic controller to calculate the linear turning case. Then a DDQN algorithm is applied for compensating the errors caused by the nonlinear feature of the vehicle and the time-varying speed. Finally, numerical validations and experimental tests in a real vehicle are conducted to verify the effectiveness of the proposed control strategy. The traditional MPC and only the DDQN method are applied as the benchmark strategies. The comparison validation results under different speed and vehicle parameters indicate that the proposed method has superior performance in adapting to time-varying speeds and vehicle nonlinear features. And the experimental tests based on actual driving scenarios also validate the remarkable stability of the developed control method.
Bayesian Earthquake Forecasting approach based on the Epidemic Type Aftershock Sequence model
Authors: Petrillo Giuseppe , Zhuang Jiancang 
Published: July 2022

Abstract
The Epidemic Type Aftershock Sequence (ETAS) model is used as a baseline model both for earthquake clustering and earthquake prediction. In most forecasting experiments, the ETAS parameters are estimated based on a short and local catalog, therefore the model parameter optimization carried out by means of a Maximum Likelihood Estimation may be not as robust as expected. We use Bayesian forecast techniques to solve this problem, where a non-informative flat prior distributions of the parameters is adopted. A Metropolis-Hasting algorithm is employed to sample the model parameters and earthquake events. We also show, through a forecasting experiment, how the Bayesian inference of the parameters allows to obtain a less confident prediction.
The oncogenic roles of JC polyomavirus T antigen in cervical cancer
Authors: Xue Hang , Zheng Huachuan , Xu Hailan , Zhang CongYu , Fan Yinjie , Ha Minwen , Cui Zhengguo 
Published: July 2022

Abstract
BackgroundAccording to pathological and transgenic data, there is a close link between JC polyomavirus (JCPyV) T antigen and various epithelial cancers. The aim of this study was to explore the role of T antigen in cervical tumorigenesis.MethodsThe phenotypes were observed in T antigen-overexpressing HeLa cells by Cell Counting Kit-8 assay, Annexin V staining, Transwell assay, and a xenograft model. Phenotype-related and T antigen partner proteins were screened by western blot. Nested, real-time, and in situ PCR were used to detect T antigen in cervical cancer.ResultsOverexpression of T antigen promoted the proliferation, anti-apoptosis, anti-pyroptosis, chemoresistance, migration, invasion, and epithelial-mesenchymal transition of HeLa cells. All mutants impaired the positively regulated effects of T antigen on proliferation. According to a general PCR, the positive rate of T antigen was higher in cervical cancer than in adjacent cervical intraepithelial neoplasia (CIN) and normal tissues (p < 0.05), in line with the JCPyV copy number (p < 0.05). T antigen-positive cells were detectable in the nuclei of cervical cancer, CIN, and squamous cells by in situ PCR. Strong nuclear immunoreactivity against T antigen was observed in cervical cancers, but not in CIN or normal squamous epithelium.ConclusionsJCPyV T antigen may insert into the genome of cervical squamous epithelial cells and encode T antigen for its oncogenesis. T antigen participates in the aggressive progression of these cells by promoting proliferation, anti-apoptosis, anti-pyroptosis, migration, invasion, epithelial-mesenchymal transition, and chemoresistance.
Lactobacillus paracasei JY062 alleviates glucolipid metabolism disorders by adipoinsular axis and gut microbiota
Authors: Jiang Yujun , Su Yue , Ren Jing , Zhang Jingwen , Zheng Jiapeng , Zhang Yu , Man Chaoxin 
Published: July 2022

Abstract
High-glucose-fat diet-induced glucolipid metabolism disorder has become a global epidemic disease. Probiotic intervention is a new strategy to alleviate metabolic syndrome. The protective effect and mechanism of L. paracasei JY062 on high-glucose-fat diets mice were investigated. After 7 weeks L. paracasei JY062 intervention, the body weight of mice in the LPH group (28.07 ± 1.07 g) was significantly lower than that of HFD group (32.03 ± 0.89 g) (P < 0.05). Among three doses of JY062, 108 and 109 CFU/mL JY062 showed the strong ability to relieve blood glucose disorders, blood lipid disorders, tissue damage. LPH group had the best effect, significantly (P < 0.05) decreased the TC (40.11%), TG (42.46%), LDL-C (39.38%), leptin (54.34%), insulin (39.95%), FFA (40.68%) concentrations and increased (P < 0.05) the HDL-C (1.05 times), adiponectin (1.53 times) and GLP-1 (1.35 times) level compared to HFD group mice. JY062 could activate the APN-AMPK pathway, increase AdipoQ, AMPK GLUT-4 and PGC-1αmRNA expression, and decrease SREBP-1c, ACC and FAS mRNA expression. L. paracasei JY062 intervention decreased the relative abundance of harmful bacteria and increased the relative abundance of beneficial bacteria, restored the imbalance of gut microbiota homeostasis caused by high-glucose-fat diet. L. paracasei JY062 could also increase the content of SCFAs in the intestines, especially butyric acid.
Elastic interfacial layer enabled the high-temperature performance of lithium-ion batteries
via utilization of synthetic fluorosulfate additive
Authors: Choi Nam-Soon , Moon Hyeongyu , Nam Huibeom , Kim Min Pyeong , Lee Seung Min , Kim Hyeongjun , Jeon Min Ho , Lee Yoon-Sung , Kim Koeun , Chun Joong-Hyun , Kwak Sang Kyu , Hong Sung You 
Published: July 2022

Abstract
Ensuring interfacial stability of Si-containing anodes and Ni-rich cathodes is the key to achieving high-energy lithium-ion cells. Herein, we present 4-(allyloxy)phenyl fluorosulfate (APFS), a multi-functional electrolyte additive that forms a mechanical strain-adaptive solid electrolyte interphase (SEI) composed of LiF and polymeric species, and a thermally stable cathode–electrolyte interface containing S–O and S–F species. Radical copolymerization of vinylene carbonate (VC) with APFS via electrochemical initiation creates a spatially deformable polymeric SEI on the Si/C-graphite (SiG-C) anode with large volume changes during cycling. Here we show that APFS-promoted interfacial layers reduce Ni dissolution and deposition and that APFS deactivates Lewis acidic PF5, inhibiting the hydrolysis that produces unwanted HF. These results show that the combined use of VC and APFS allows capacity retention of 72.5% with a high capacity of 143.5 mAh g− 1 after 300 cycles at 45°C.
LINC00472 inhibits cell migration by enhancing intercellular adhesion and regulates H3K27ac level via interacting with P300 in Renal Clear Cell Carcinoma
Authors: Wang Songmao , Luo Cheng , Li Bing , Zhang Shikuan , Liao Weijie , Xin Qilei , Xu Naihan , Xie Weidong , Zhu Yuanchang , Zhang Ya-ou 
Published: July 2022

Abstract
Renal clear cell carcinoma (RCCC) is the most common type of renal cell carcinoma, which is also difficult to diagnose and easy to metastasize. Currently, there is still a lack of effective clinical diagnostic indicators and treatment targets. This study aims to find effective diagnostic markers and therapeutic targets from the perspective of noncoding RNA. In this study, we found that the expression of Long noncoding RNA LINC00472 was significantly decreased in RCCC and showed a downward trend with the progression of cancer stage. Patients with low LINC00472 expression have poor prognosis. Inhibition of LINC00472 significantly increased cell proliferation and migration, while overexpression of LINC00472 obviously inhibited cell proliferation and enhanced intercellular adhesion. Transcriptome sequencing analysis demonstrated that LINC00472 was highly correlated with extracellular matrix and cell metastasis related pathways, and the consistent results were obtained by The Cancer Genome Atlas (TCGA) data analysis. Additionally, we discovered that the integrin family protein ITGB8 is a target gene of LINC00472. Mechanistically, we identified that LINC00472 interacts with histone acetyltransferase P300 to regulate the acetylation level of H3K27 at the ITGB8 transcription start site, which is the first time to elucidate its function in the regulation of histone modification. Our analysis results illustrated that LINC00472 can be used as a potential diagnosis marker of RCCC, and our mechanism researches provide a theoretical basis for the clinical development of new and effective treatment strategies.
Chemotherapy and targeted therapy for advanced biliary tract cancers：An umbrella review
Authors: Wang Yaoqun , Wen Ningyuan , Wang Shaofeng , Nie Guilin , Tian Yuan , Lu Jiong , Li Bei 
Published: July 2022

Abstract
Background: Malignant tumors of the biliary system have high malignancy and strong invasiveness, and are usually found in late stages with poor prognosis. For patients with advanced biliary tract cancer, chemotherapy and targeted therapy are one of the options to improve prognosis and delay tumor progression. The purpose of this study was to comprehensively evaluate the safety and efficacy of various chemotherapy regimenfor advanced biliary carcinoma. Methods : An umbrella review method was adopted, which aims to summarize the evidence from multiple studies around a research topic. Evidence from meta analyses up to 09 April 2022 were identified using PubMed, Web of Science, the Cochrane database, as well as manual screening. Eligible studies were screened according to inclusion and exclusion criteria. This study had been registered at PROSPERO (CRD42022324548).For each eligible study, we extracted the data of general characteristics and the main findings. The methodological quality of the included studies were assessed by AMSTAR2 and the quality of evidence was evaluated by the GRADE. Results : A total of 1833 articles were searched, 14 unique articles with 94 outcomes were identified by eligibility criteria. The incidence of skin rash (RR=18.11,95%CI 5.13-63.91) and diarrhea (RR=2.48,95%CI 1.2-5.10) was higher in patients receiving gemcitabine-based chemotherapy plus targeted therapy than in patients receiving gemcitabine alone. Patients receiving gemcitabine-containing chemotherapy developed leukopenia (OR=7.17, 95%CI 1.43-36.08), anemia (OR=7.04, 95%CI 2.59-19.12), thrombocytopenia (RR=2.45, 95%CI 1.39-4.32) and neutropenia (RR=3.30, 95%CI 1.04-10.50) were significantly higher than gemcitabine-free regimens. In addition, patients receiving S-1 monotherapy had significantly better ORR (RR=2.46,95%CI 1.27-4.57) than patients receiving S-1+gemcitabine. Patients receiving fluoropyrimidine-based chemotherapy had longer OS (HR=0.83, 95%CI 0.7-0.99), higher DCR (0R=5.18, 95%CI 3.3-10.23), and higher ORR (0R=3.24, 95%CI 1.18-8.92) compared with 5-FU/LV monotherapy or supportive therapy. Surprisingly, there was also evidence that gemcitabine-based chemotherapy did not improve postoperative patients' OS (HR=0.91, 95%CI 0.74-1.12) when compared with best supportive care. Conclusions: This study comprehensively evaluated the safety and efficacy of chemotherapy or targeted therapy regimens for advanced biliary tract cancer and found 11 "moderate" or "high" levels of evidence, however, most of the evidence was still at "low" or "very low" levels. More randomized controlled studies are needed in the future to further summarize high-level evidence.
SGOL2 is a novel prognostic marker and fosters disease progression via a MAD2-mediated pathway in hepatocellular carcinoma
Authors: HU Qingqing , Liu Qiuhong , Zhao Yalei , Zhang Lingjian , Li Lanjuan 
Published: July 2022

Abstract
Background: Shugoshin-like protein 2 (SGOL2) is a centromeric protein that ensures the correct and orderly process of mitosis by protecting and maintaining centripetal adhesions during meiosis and mitosis. Here, we examined the potential role of SGOL2 in cancers, especially in hepatocellular carcinoma (HCC).Methods: 199 normal adjacent tissues and 202 HCC samples were collected in this study. Human HCC cells (SK-HEP-1 and HEP-3B) were employed in the present study. Immunohistochemistry, immunofluorescence, western blot, Co-Immunoprecipitation technique, and bioinformatic analysis were utilized to assess the role of SGOL2 in HCC development process.Results: Overexpression of SGOL2 predicted an unfavorable prognosis in HCC by The Cancer Genome Atlas database (TCGA), which were further validated in our two independent cohorts. Next, 47 differentially expressed genes positively related to both SGOL2 and MAD2 were identified to be associated with the cell cycle. Subsequently, we demonstrated that SGOL2 downregulation suppressed the malignant activities of HCC in vitro and in vivo. Further investigation showed that SGOL2 promoted tumor proliferation by regulating MAD2-induced cell-cycle dysregulation, which could be reversed by the MAD2 inhibitor M2I-1. Consistently, MAD2 upregulation reversed the knockdown effects of SGOL2-shRNA in HCC. Moreover, SGOL2 regulates MAD2 by directly binding with MAD2. Conclusion: SGOL2 acts as an oncogene in HCC cells by directly regulating MAD2 and then dysregulating the cell cycle, providing a potential therapeutic target in HCC.
Evolutionary differentiation of androgen receptor ohnologs is responsible for the development of androgen-dependent unique sexual characteristics in a teleost fish
Authors: Ogino Yukiko , Ansai Satoshi , Watanabe Eiji , Yasugi Masaki , Katayama Yukitoshi , Sakamoto Hirotaka , Okamoto Keigo , Okubo Kataaki , Yamamoto Yasuhiro , HARA Ikuyo , Yamazaki Touko , Kato Ai , Kamei Yasuhiro , Naruse Kiyoshi , Ohta Kohei , Ogino Hajime , Sakamoto Tatsuya , Miyagawa Shinichi , SATO TOMOMI , Yamada Gen , Baker Michael , Iguchi Taisen 
Published: July 2022

Abstract
Teleost fishes exhibit complex unique sexual characteristics, such as fin enlargement and courtship display, in response to androgens. However, the molecular mechanisms underlying their evolutionary acquisition remain largely unknown. To address this question, we analysed medaka (Oryzias latipes) mutants deficient in androgen receptor ohnologs (ara and arb) generated by the teleost-specific whole-genome duplication event (TSGD). We discovered that both ar ohnologs are not required for spermatogenesis and appear to be functionally redundant for courtship display in males, while both copies were necessary for their reproductive success; ara was required for tooth enlargement and behavioural attractiveness, while arb for male-specific fin morphogenesis and sexual motivation. We further showed that the differences in both the transcription of the two ars, cellular localisation of their encoded proteins and their downstream genetic programs could be responsible for the phenotypic diversity between the ara and arb mutants. These findings suggest that the ar ohnologs have diverged in the teleost lineage in two different ways: First through the loss of their roles in spermatogenesis and second through the gene duplication followed by functional differentiation that has likely resolved the pleiotropic roles derived from their ancestral gene. Thus, our results provide insights into how genome duplication impacts the massive diversification of sexual characteristics in the teleost lineage.