bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative anaemia and red blood cell transfusion in patients with aneurysmal subarachnoid and intracerebral haemorrhage — a multicentre subanalysis of the German PBM Network Registry
Authors: Schmitt Elke , Meybohm Patrick , Neef Vanessa , Baumgarten Peter , Bayer Alexandra , Choorapoikayil Suma , Friederich Patrick , Friedrich Jens , Geisen Christof , Güresir Erdem , Grünewald Matthias , Gutjahr Martin , Helmer Philipp , Herrmann Eva , Müller Markus , Narita Diana , Raadts Ansgar , Schwendner Klaus , Seifried Erhard , Stark Patrick , Steinbicker Andrea U. , Thoma Josef , Velten Markus , Weigt Henry , Wiesenack Christoph , Wittmann Maria , Zacharowski Kai , Piekarski Florian ,   German PBM Network Collaborators
Published: February 2022

Abstract
Purpose
Anaemia is common in patients presenting with aneurysmal subarachnoid (aSAH) and intracerebral haemorrhage (ICH). In surgical patients, anaemia was identified as an idenpendent risk factor for postoperative mortality, prolonged hospital length of stay (LOS) and increased risk of red blood cell (RBC) transfusion. This multicentre cohort observation study describes the incidence and effects of preoperative anaemia in this critical patient collective for a 10-year period.

Methods
This multicentre observational study included adult in-hospital surgical patients diagnosed with aSAH or ICH of 21 German hospitals (discharged from 1 January 2010 to 30 September 2020). Descriptive, univariate and multivariate analyses were performed to investigate the incidence and association of preoperative anaemia with RBC transfusion, in-hospital mortality and postoperative complications in patients with aSAH and ICH.

Results
A total of n = 9081 patients were analysed (aSAH n = 5008; ICH n = 4073). Preoperative anaemia was present at 28.3% in aSAH and 40.9% in ICH. RBC transfusion rates were 29.9% in aSAH and 29.3% in ICH. Multivariate analysis revealed that preoperative anaemia is associated with a higher risk for RBC transfusion (OR = 3.25 in aSAH, OR = 4.16 in ICH, p < 0.001), for in-hospital mortality (OR = 1.48 in aSAH, OR = 1.53 in ICH, p < 0.001) and for several postoperative complications.

Conclusions
Preoperative anaemia is associated with increased RBC transfusion rates, in-hospital mortality and postoperative complications in patients with aSAH and ICH.

Trial registration
ClinicalTrials.gov, NCT02147795, https://clinicaltrials.gov/ct2/show/NCT02147795
Synthesis of [7-15N]-GTPs for RNA structure and dynamics by NMR spectroscopy
Authors: Taiwo Kehinde M. , Olenginski Lukasz T. , Nußbaumer Felix , Nam Hyeyeon , Hilber Stefan , Kreutz Christoph , Dayie T. Kwaku 
Published: February 2022

AbstractSeveral isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-15N]-guanosine-5′-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs.
Graphical abstract
Improving the microbial sampling and analysis of secondary infected root canals by passive ultrasonic irrigation
Authors: Kluge Milena , Trüschler Johanna , Elamin Fadil , Anderson Annette , Hellwig Elmar , Altenburger Markus , Vach Kirstin , Wittmer Annette , Al-Ahmad Ali 
Published: February 2022

Abstract
Objective
The persistence of pathogenic microorganisms in root canals is the most common reason for the failure of root canal treatment and the necessity of a root filling treatment, which results in an uncertain prognosis due to technical complexity and the variety of highly adaptable microorganisms.
This study evaluated the effect of passive ultrasonic irrigation (PUI) on the outcome of the microbial analysis of root canal-treated teeth with persistent or recurrent apical inflammation in vivo.

Materials and methods
Sample collection was performed after root filling removal (sample S1, control group) and after PUI with NaCl (sample S2) using sterile paper points. In total, 19 samples were obtained. Quantification was performed by means of serial dilution of the samples. Subcultivated pure cultures were identified using MALDI-TOF MS complemented by the Vitek-2-System or PCR, followed by sequencing of the 16S rRNA gene. The results of the samples (S1 and S2) were evaluated regarding their bacterial count and composition.

Results
The total count of bacteria and the number of aerobic/facultative anaerobic microorganisms significantly increased in the S2-samples after application of PUI. The number of obligate anaerobic microorganisms showed an increase after PUI, although it was not significant. We detected 12 different aerobic/facultative anaerobic microorganisms before PUI, and in 21 cases after PUI. Two different obligate anaerobic microorganisms were found in S1 samples compared to nine different species in S2 samples.

Conclusions
PUI is a powerful method for detaching bacteria in infected root canals and enables a more precise analysis of the etiology of persistent endodontic infections.

Clinical relevance
This study indicates that PUI exerts a positive cleansing effect and adds to the accessibility of microorganisms during the application of bactericidal rinsing solution in root canal treatments.
Parameterized verification of systems with component identities, using view abstraction
Authors: Lowe Gavin 
Published: February 2022

AbstractThe parameterized verification problem seeks to verify all members of some collection of systems. We consider the parameterized verification problem applied to systems that are composed of an arbitrary number of component processes, together with some fixed processes. The components are taken from one or more families, each family representing one role in the system; all components within a family are symmetric to one another. Processes communicate via synchronous message passing. In particular, each component process has an identity, which may be included in messages, and passed to third parties. We extend Abdulla et al.’s technique of view abstraction, together with techniques based on symmetry reduction, to this setting. We give an algorithm and implementation that allows such systems to be verified for an arbitrary number of components: we do this for both safety and deadlock-freedom properties. We apply the techniques to a number of examples. We can model both active components, such as threads, and passive components, such as nodes in a linked list: thus our approach allows the verification of unbounded concurrent datatypes operated on by an unbounded number of threads. We show how to combine view abstraction with additional techniques in order to deal with other potentially infinite aspects of the analysis: for example, we deal with potentially infinite specifications, such as a datatype being a queue; and we deal with unbounded types of data stored in a datatype.
Comparison of antimicrobial and wound-healing effects of silver nanoparticle and chlorhexidine mouthwashes: an in vivo study in rabbits
Authors: Moaddabi Amirhossein , Soltani Parisa , Rengo Carlo , Molaei Sahar , Mousavi Seyed Jaber , Mehdizadeh Mojdeh , Spagnuolo Gianrico 
Published: February 2022

AbstractThe objective is to formulate a silver nanoparticle mouthwash and then evaluate its antimicrobial and wound-healing effects in rabbit animal models. Microbial samples were collected from the oral cavity of 60 rabbits. Thereafter, standardized wounds were created in the lateral border of the tongue on the right side for all rabbits. After surgery, digital photographs were obtained from the wounds with standardized settings. To characterize the silver nanoparticles used in the synthetic mouthwash, transmission electron microscopy (TEM) and digital light scattering analysis were used. The animal models were then randomly divided into 4 groups: group 1 received 9.80 wt% silver nanoparticle mouthwash; group 2 received all the ingredients of the formulated mouthwash except for silver nanoparticles; group 3 received chlorhexidine 2.0% mouthwash; and the negative control group did not receive any postoperative mouthwash. Microbial samples were collected from oral cavity of the rabbits each day for four postoperative days. Colony-forming unit (CFU) counts were compared post-operatively with the pre-operative counts. In addition, standardized digital photographs were taken each day from the wounds and the area of the wounds was compared in postoperative and pre-operative images. Data were statistically analyzed using one-way ANOVA and repeated measures variance analysis (α = 0.05). TEM revealed spherical morphology of silver nanoparticles and digital light scattering showed an average size of 5 nm and optimal distribution of the nanoparticles. CFU count significantly decreased in groups 1 and 3 (P < 0.001), while it significantly increased in groups 2 and 4 (P < 0.001). Moreover, a significant difference was observed between the experimental groups (P < 0.001). In addition, wound area decreased significantly in all groups (P < 0.001). However, the difference between wound areas in the groups was not significant, except for the 4th postoperative day (P < 0.001). However, the antibacterial effects and the wound-healing characteristics of the synthetic silver nanoparticle and chlorhexidine mouthwashes were not significantly different (P > 0.05). Silver nanoparticle mouthwash possesses favorable antibacterial and wound-healing effects. The formulated 9.80 wt% silver nanoparticle mouthwash with a particle size of 5 nm can be a promising alternative for application after oral surgical procedures.
Development of 3D Printed Mitral Valve Constructs for Transcatheter Device Modeling of Tissue and Device Deformation
Authors: Vukicevic Marija , Mehta Shail Maharshi , Grande-Allen K. Jane , Little Stephen H. 
Published: February 2022

AbstractTranscatheter mitral valve repair (TMVR) therapies offer a minimally invasive alternative to surgical mitral valve (MV) repair for patients with prohibitive surgical risks. Pre-procedural planning and associated medical device modeling is primarily performed in silico, which does not account for the physical interactions between the implanted TMVR device and surrounding tissue and may result in poor outcomes. We developed 3D printed tissue mimics for modeling TMVR therapies. Structural properties of the mitral annuli, leaflets, and chordae were replicated from multi-material blends. Uniaxial tensile testing was performed on the resulting composites and their mechanical properties were compared to those of their target native components. Mimics of the MV annulus printed in homogeneous strips approximated the tangent moduli of the native mitral annulus at 2% and 6% strain. Mimics of the valve leaflets printed in layers of different stiffnesses approximated the force–strain and stress–strain behavior of native MV leaflets. Finally, mimics of the chordae printed as reinforced cylinders approximated the force–strain and stress–strain behavior of native chordae. We demonstrated that multi-material 3D printing is a viable approach to the development of tissue phantoms, and that printed patient-specific geometries can approximate the local deformation force which may act upon devices used for TMVR therapies.
Extent and hierarchy of seismic induced inelastic demands in the substructure system of bridges on piled foundation crossing waterways
Authors: Tawadros H. W. S. , Farag M. M. N. , Mehanny S. S. F. 
Published: February 2022

AbstractThe effect of the exposed length of piles supporting bridges crossing waterways on the spread of inelasticity along the pile shaft compared with the extent of plasticity in the body of the column, and hence on the precedence of the formation of plastic hinges in the substructure system is of paramount importance. Plastic hinges may form ideally and preferably at the bottom of the column just above the pile cap, or undesirably either at the pile’s head or at any other section within the pile be it along its free/exposed length immersed in water or its embedded part in soil. A set of bridges are designed according to Eurocode for multiple configurations with various relative stiffness between the column and the group of piles featuring partly exposed shaft. Pushover as well as time history inelastic analyses under a set of ten earthquake records are performed accounting for soil-structure interaction. Results demonstrate that it is likely in some cases characterized by a remarkable increase in the flexural stiffness of the column relative to the group of piles to have spread of inelasticity and plastic hinges forming in piles prior to the column. This is undesirable for a robust, reliable and resilient seismic design as devised by universal design standards for bridges on piled foundation. While there is no clear consensus on the most effective corrective measure to such undesirable response, a few proposed remedial design actions have been formulated in order to preclude (or at least improve the behavior for the case featuring) plastic hinges deplorably forming in the piles prior to the base of the column.
ATGL deficiency aggravates pressure overload-triggered myocardial hypertrophic remodeling associated with the proteasome-PTEN-mTOR-autophagy pathway
Authors: Han Xiao , Zhang Yun-Long , Lin Qiu-Yue , Li Hui-Hua , Guo Shu-Bin 
Published: February 2022

AbstractPersistent myocardial hypertrophy frequently leads to heart failure (HF). Intramyocardial triacylglycerol (TAG) accumulation is closely related with cardiac remodeling and abnormal contractile function. Adipose triglyceride lipase (ATGL), a key enzyme in TAG metabolism, regulates cardiac function. However, its associated molecular pathways have not been fully defined. Here, cardiac hypertrophy and HF were induced in wild-type (WT) or ATGL knockout (KO) mice through transverse aortic constriction (TAC) for up to 4 weeks. TAC in WT mice significantly reduced cardiac function and autophagy while enhancing left ventricular hypertrophy, interstitial fibrosis, inflammatory response, superoxide generation, and cardiomyocyte apoptosis, accompanied with upregulation of the proteasome activity, reduction of PTEN level and activation of AKT-mTOR signaling, and these effects were further aggravated in ATGL KO mice. Interestingly, ATGL KO-mediated cardiac dysfunction and remodeling were markedly reversed by proteasome inhibitor (epoxomicin) or autophagic activator (rapamycin), but accelerated by PTEN inhibitor (VO-OHpic) or autophagy inhibitor 3-MA. Mechanistically, ATGL KO upregulated proteasome expression and activity, which in turn mediates PTEN degradation leading to activation of AKT-mTOR signaling and inhibition of autophagy, thereby enhancing hypertrophic remodeling and HF. In conclusion, ATGL KO contributes to TAC-induced cardiac dysfunction and adverse remodeling probably associated with the proteasome-PTEN-mTOR-autophagy pathway. Therefore, modulation of this pathway may have a therapeutic effect potential for hypertrophic heart disease.
Graphical abstract
TAC-induced downregulation of ATGL results in increased proteasome (β1i/β2i/β5i) activity, which in turn promotes degradation of PTEN and activation of AKT-mTOR signaling and then inhibits autophagy and ATP production, thereby leading to cardiac hypertrophic remodeling and dysfunction. Conversely, blocking proteasome activity or activating autophagy attenuates these effects.
Attitudes of Catholic Priests Regarding the Participation of People with Schizophrenia and Depression in Religious Practices: Relationships with Prejudices and Community Size
Authors: Magliano Lorenza , Affuso Gaetana 
Published: February 2022

AbstractThis study investigated whether priests’ attitudes regarding individuals with schizophrenia and depression participating in religious practices varied in relation to priests’ adherence to prejudices about these mental disorders (MD). A sample of 559 Italian priests completed a questionnaire on their views of either schizophrenia or depression. Data were analyzed using a multiple-group structural equation in which the diagnostic group was a moderator and the size of the municipalities in which the churches were located was a covariate. The study revealed that: priests’ attitudes towards churchgoers with MDs are related to views of these individuals as dangerous, easy to recognize and poorly aware of their MDs; community size has a direct effect on priests’ attitudes and an indirect effect through perceived dangerousness; the above-mentioned relationships do not differ by type of disorder. Sensitizing priests on stigma may be helpful to facilitate the participation of believers with MDs to religious practices.
Development, Construct Validation, and Normalization of a New Early Childhood Self-Regulation Assessment Scale
Authors: Boyer Wanda 
Published: February 2022

AbstractAlthough there are many tools for assessing young children’s self-regulation according to varied conceptual definitions and purposes, the purpose of this study was to develop, validate, and norm a Self-Regulation Assessment Scale for Early Childhood (SASEC) for directly evaluating observed behaviors of young children in naturalistic play experiences within the normal preschool environment. An exploratory sequential mixed methods research design was used. The 315 participants included 153 parents and 15 educators for the qualitative component and 147 children ages 3–5 years for the quantitative component. The analytical steps of a qualitative grounded theory research design were applied to adult participant interviews and focus group discussions, which culminated in 12 scale items for measuring a child’s ability to initiate, modulate, and cease behaviors, tasks, or activities of varied complexities, social configurations, and limiting conditions. Children’s SASEC scores were assessed via video recordings of play behaviors in naturalistic settings. Based on factor analysis results, the SASEC items constitute a single construct. According to the results of hierarchical linear modeling and multiple linear regression, preschool children’s SASEC scores can be compared to the SASEC mean and standard deviation regardless of various demographic variables. Implications and recommendations for future work include having early childhood educators, child and youth care practitioners, counselors, parents and families, social workers, behavioral sciences researchers, and policy makers use the SASEC to measure young children’s self-regulation while developing or monitoring the efficacy of generalized enhancement programs and individualized treatment plans.
Surface Tension and Thermal Conductivity of NIST SRM 1155a (AISI 316L Stainless Steel)
Authors: Pichler Peter , Leitner Thomas , Kaschnitz Erhard , Rattenberger Johannes , Pottlacher Gernot 
Published: February 2022

AbstractNIST SRM 1155a is an AISI 316L stainless steel (Cr18–Ni12–Mo2) and Standard Reference Material (SRM) intended for use with test methods for elemental analysis. In a previous paper “Measurement of thermophysical properties of solid and liquid NIST SRM 316L stainless steel”, we already published reliable thermophysical properties of high temperature solid and of the liquid phase of this material such as temperature dependent enthalpy, density and electrical resistivity, as well as specific heat capacity for the solid and the liquid phase. In this paper, we add additional thermophysical properties obtained by ohmic pulse-heating and by the laser flash method, namely thermal conductivity and thermal diffusivity as a function of temperature. Furthermore we report surface tension measurement results of liquid SRM 1155a obtained by means of electromagnetic levitation. Simulation of processes like additive manufacturing, laser welding, laser cutting or metal casting depend on the above named quantities as input data. Ohmic pulse-heating as well as electromagnetic levitation are so called “containerless” investigation techniques and no significant chemical reactions of the hot liquid alloy with its surrounding occur. The data presented here are compared to the available literature data and are accompanied by an uncertainty analysis according to the “Guide to the Expression of Uncertainty in Measurement”.
Performance Assessment of OTFS Modulation in High Doppler Airborne Communication Networks
Authors: Chu Thi My Chinh , Zepernick Hans-Jürgen , Westerhagen Alexander , Höök Anders , Granbom Bo 
Published: February 2022

AbstractEmerging 5G and future 6G mobile networks are expected to cater for high mobility scenarios ranging from vehicle-to-vehicle communications to unmanned aerial vehicles and airborne platforms. Communications in this type of deployments suffer from severe Doppler shifts which require new modulation waveforms. Orthogonal time frequency space (OTFS) modulation has recently been proposed as a promising technology for coping with high Doppler channels. OTFS converts a time-varying fading channel into a time-independent channel in the two-dimensional delay-Doppler (DD) domain. The transmit symbols are multiplexed into a nearly constant channel with a complex channel gain in the DD domain. In this paper, we consider a high Doppler airborne communication network where relative mobile node speeds can be above 1200 m/s. The considered system represents a mobile ad-hoc network where the airborne mobile nodes can join or leave the network. Furthermore, each node is equipped with an antenna array that supports directed communication among mobile nodes. The Doppler shifts in this airborne communication network are in the order of 52-72 kHz and may potentially be even higher depending on the selected carrier frequency and the relative speed among the airborne platforms. As such, OTFS modulation is used in this work to efficiently compensate for the high Doppler shifts in the DD domain. In particular, a comprehensive performance assessment in terms of bit error rate (BER) is conducted to reveal the potential of OTFS modulation in dealing with such extreme transmission scenarios. The impact of physical layer parameters, number of delay-Doppler bins in the DD domain used for OTFS modulation, directed versus two-ray channels, and the combination of multiple-input multiple-output (MIMO) systems with OTFS modulation on the BER is assessed. It is shown that both OTFS modulation over a two-ray channel as well as MIMO-OTFS modulation provide a reliable airborne communication network with low BER.