bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gender Gaps and Family Policies in Latin America
Authors: Galván Estefanía , Parada Cecilia , Querejeta Martina , Salvador Soledad 
Published: October 2022

Abstract
Gender equality in the labor market remains a difficult challenge in Latin America and recent literature shows that child penalties play an important role in explaining these gaps. While policies to address gaps related to parenthood were introduced in recent decades, evidence of its effects is still scarce. This paper presents comparable evidence on the adoption of family legislation in 15 Latin American countries and discusses its relationship with the evolution of the gender gaps in the labor market and the prevailing gender norms. We document that from 2000 to 2019 almost all countries increased the weeks covered by maternity, paternity, or parental leaves. Following a similar approach to that of Olivetti and Petrongolo (2017), we exploit the variations over time and control for country and year-fixed effects to study the relationship between the extension of family leaves and women's outcomes. We find that these policies are successful in increasing female employment and reducing employment gaps in countries departing from a worse situation in terms of leave coverage or with more traditional perceptions of gender roles. On the other hand, for countries which have more egalitarian gender perceptions, our results suggest that the extension of family leaves contributes to reduce the income gaps.
Characterisation of a New Lightweight Lorawan Gps Biologger and Deployment on Griffon Vultures Gyps Fulvus
Authors: Gauld Jethro , Atkinson Philip W. , Silva João P , Senn Andreas , Franco Aldina M. A. 
Published: October 2022

Abstract
1. Information provided by tracking studies using satellite telemetry is providing ecologists with invaluable new insights into animal behaviour and movement strategies. Here we describe a new type of GPS (global positioning system) tracking device which makes use of a growing network of LoRaWAN (long range, low power wide area network) gateways. These tags have the potential to be a low weight and power consumption solution. 2. We characterise the GPS accuracy and data transmission range, including uplinks and downlinks, for the tracker using a series of standardised tests. Data transmission range was tested by visiting locations with line of sight to the LoRa gateway at distances up to 75km and recording whether data transmission was completed successfully from each location. These tests were complemented by a trial deployment of six devices on Griffon Vultures Gyps fulvus. 3. These LoRa tags reliably provided accurate GPS location estimates, particularly on shorter location acquisition cycles. At one-minute intervals the GPS location bias was 4.71m in the horizontal plane and 5m in the vertical plane while GPS precision, measured by standard deviation, was 3.9m in horizontal space and 7.7m in vertical space. GPS locations were less accurate on a longer acquisition cycle but still comparable with other commercially available tags. Ground based range tests revealed reliable transmission of multiple data payloads was recorded from a maximum distance of 40.7km. Initial results from a deployment on Griffon Vultures Gyps fulvus yielded useful information about flight speeds, height and transmission range during the first two weeks after tag deployment. 4. The LoRa tags demonstrated potential for effective use in the study of animal movement. The small size and power needs allows for flexibility in which combination of battery, solar panel, and housing they are paired with meaning that fully assembled tags can weigh less than 5g. The LoRaWAN gateway network does not yet allow global coverage, hence at present, this solution is best suited to the study of colonial species, where gateways can be installed on site, or species with consistent migration routes.
A new anthropometric model for body composition estimation in the assessment of metabolic risk factors of obese women
Authors: Dahlmann Nicolaus , Klingmüller² Dietrich 
Published: October 2022

Abstract

Background
Excessive body fat is associated with disorders defined as Metabolic Syndrome (MetS). The study sets out to uncover the underlying mechanisms of MetS pathogenesis comparing metabolic and inflammatory variables with increasing amounts of fat mass (FM). Aiming to solve this issue, the study refers to an anthropometric model named Dahlmann-Body-Analysis (DBA) to define the percentage of fat mass (%FM).
Methods
A data set of 61 severely obese women were analysed. All subjects had a BMI > 30 kg/m². Body weight (W), body height (Ht), hand circumference (HdC) and the circumference of the abdomen (AC) were measured and processed by the DBA model. The result is the percentage of fat mass (%FM), which is compared to data, produced by a bioelectrical impedance analysis (BIA) device. Anthropometric data are statistically compared with systolic blood pressure (SBP) and the MetS risk factors triglyceride (TG), HDL cholesterol (HDL-C), fasting plasma glucose (FPG) and the parameters C-reactive protein (CRP) and low-density lipoprotein (LDL-C) using receiver operating curves (ROC) based on sensitivity and specificity, area under curve (AUC), correlation coefficients and regression analysis.
Results
The average %FM was about 50%, meaning that 44% of subjects suffered from MetS. The overall pattern of correlation coefficients revealed that none of the adiposity indices like BMI, AC, AC/Ht and %FM (BIA or DBA) is of crucial advantage to detect metabolic risk factors. AUC values of the different obesity indices detecting MetS reached values between 0.63 and 0.75 representing a low discrimination power in the diagnose of MetS. Associations between body fat mass measured by the DBA system (%FM-DBA) and the systolic blood pressure and seven metabolic risk factors showed a significantly rising linear relationship for the parameters Insulin, HOMA-IR, HDL-C and CRP. The corresponding correlation coefficients are r > 0.30. The parameters Glucose, TG, LDL-C and SBP had correlation coefficients r < 0.12.
Conclusions
To our knowledge, it is the first time that biochemical parameters and blood pressure are associated with increasing amounts of fat mass in human adults. The waist circumference (WC) as part of the MetS definition should be replaced by direct or indirect measurements of body fat estimation to give reliable information on individuals.
Construction and validation of a cuproptosis-related lncRNA signature for the prediction of the prognosis of LUAD and LUSC
Authors: Wang Yu , Xiao Xu , Li Yan 
Published: October 2022

Abstract
Lung cancer is one of the most prevalent malignant tumors worldwide, with lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) accounting for the majority of cases. Cuproptosis, tumor immune microenvironment (TIME) and long non-coding RNA (lncRNA) have been demonstrated to be associated with tumorigenesis. The objective of the present study was to develop a novel cuproptosis-related lncRNA signature to assess the association between cuproptosis and TIME in patients with LUAD or LUSC and to predict prognosis. Based on the outputs of the least absolute shrinkage and selection operator regression model, a cuproptosis-related lncRNA signature was developed. Kaplan-Meier survival curves were generated to confirm the predictive ability of the signature. Univariate and multivariate analysis was also performed to determine the association between overall survival and this signature and other clinical characteristics, and a nomogram was created. Additionally, the relationship between the signature, TIME, tumor mutation burden and m6A methylation was established. The results of the present study revealed that 8 cuproptosis-related lncRNAs were associated with the prognosis of patients with LUAD and LUSC. This novel cuproptosis-related lncRNA signature is associated with TIME and m6A methylation in LUAD and LUSC and can predict prognosis with accuracy.
The interplay between NLRP3 and S1P in Egyptian Metastatic and Non- Metastatic Breast Cancer Patients and their potential diagnostic implications
Authors: Soltan Anas R. , kamal Amany M. , El-Shimy Reham A.A. , El-Mesallamy Hala O. 
Published: October 2022

Abstract
Background
Breast cancer (BC) is the most commonly diagnosed cancer and is considered the main cause of death in women worldwide. Metastatic BC is a state when cancerous cells spread to other tissues, including lung, bone, liver and brain. Sphingosine 1-phosphate (S1P) is emerging as a key regulator of proliferation, inflammation, vasculogenesis and resistance to apoptotic cell death. There is increasing evidence of a role of S1P receptors [e.g. sphingosine phosphate 4 (S1P4) and sphingosine kinase 1 (SK1)] in cancer, where overexpression of these proteins in estrogen receptor (ER) negative BC patients is linked to aggressive disease and poor prognosis. It has been discovered that the bioactive lipid metabolite S1P can act as a damage-associated molecular patterns (DAMP), and in vitro, it induced Nod-like receptor 3 (NLRP3) dependent activation of caspase-1 and secretion of interleukin-1b (IL-1b).
Objective
evaluate serum levels of S1P and NLRP3 to clarify the role of these markers in metastatic and non-metastatic BC patients and examine their potential as reliable novel diagnostic biomarkers.
Methods
The study involved 26 Egyptian female patients diagnosed with metastatic BC (stage 4) and 30 Egyptian female patient diagnosed with non-metastatic BC (stages 0, 1, 2 and 3). NLRP3, S1P and cancer antigen15.3 (CA 15.3) serum levels were analyzed using ELISA technique.
Results
The results revealed significantly lower serum levels of NLRP3 and S1P in non-metastatic and metastatic BC Egyptian patients compared to the healthy Egyptian female-control group (P < 0.001). ROC curve analysis showed that S1P has a promising good diagnostic utility in late-stage BC while NLRP3 has a moderate diagnostic utility in early and late BC.
Conclusion
NLRP3 and S1P could be promising novel diagnostic biomarkers of BC.
Knowledge of nosocomial infections, standard precautions, and source of information among physiotherapy undergraduates in Sri Lanka; an observational study
Authors: Rubasinghe Sahan , Priyadarshani Kokila , Wijesundara Pramodha , Ramyamala Singappulige , Lakmal Krishantha , Bandara Anuradhi , Dasanayaka Renuka 
Published: October 2022

Abstract
Background:
The physiotherapists are one of the first contact health care professionals’ and physiotherapy undergraduates should have sufficient knowledge of nosocomial infections, standard precautions, and hand hygiene even during their training period. Hence the purpose of this study was to assess the knowledge of nosocomial infections, standard precautions, and source of information among physiotherapy undergraduates in Sri Lanka.
Methods:
Descriptive cross sectional Google based survey study was conducted with the participation of 294 of physiotherapy undergraduates in University of Peradeniya (n = 103), University of Colombo (n = 103), and Kotalawala Defense University (n = 88). Self-constructed data sheets for socio-demographic and source of information and Infection Control Standardized Questionnaire consisted with 3 domains; knowledge of nosocomial infections, standard precautions and hand hygiene was used for data collection.
Results:
76.50% (n = 225) participants have achieved adequate level of total knowledge and have average knowledge 67.14 ± 16.831, 84.41 ± 14.679 and 66.41 ± 15.408 on the nosocomial infections, standard precautions and hand hygiene respectively. 28.31% (n = 83) have equally mentioned, formal teaching at faculty and informal sources as the most important source of knowledge. There was no significant impact of the university and the duration of clinical exposure on knowledge of nosocomial infections, standard precautions, hand hygiene and total knowledge. The study year have a significant impact and the final year undergraduates have higher knowledge compare with other study years. Female group has significant impact on total knowledge.
Conclusion:
Knowledge of nosocomial infections and infection control measures are satisfactory among the physiotherapy undergraduates in Sri Lanka. Nevertheless, for further development of formal sources of information on nosocomial infections are recommended.
Predictors for metamorphopsia in eyes undergoing macular hole surgery
Authors: Takeyama Asuka , Imamura Yutaka , Fujimoto Taichi , Iida Toshiya , Komiya Yuko , Shibata Masaki , Ishida Masahiro 
Published: October 2022

Abstract
Metamorphopsia is an important visual symptom of macular disease. We determined predictors for metamorphopsia investigating the relationships of macular hole (MH) diameter and retinal layer thicknesses with metamorphopsia after MH surgery. Forty-two eyes of 42 consecutive patients undergoing MH surgery were retrospectively studied. Metamorphopsia was measured with M-CHARTS. Inner nuclear layer (INL) and outer retinal layer (OR) thicknesses were measured 1000 µm away from central fovea at using Spectralis. Preoperative M-CHARTS scores correlated with MH diameters (P = 0.007–0.031) and changes of temporal OR thickness (P = 0.008–0.010). Postoperative M-CHARTS score at 3 months correlated with preoperative nasal and inferior OR thicknesses (P = 0.003 and 0.016) and with changes of superior INL at 3 and 6 months (P = 0.011 and 0.025), and score at 1 month with change of temporal OR at 6 months (P = 0.033). Postoperative improvement of M-CHARTS scores correlated with changes of temporal INL and superior OR (P = 0.026 and 0.002). Multiple regression analysis revealed that MH diameter was a significant predictor for metamorphopsia. Photoreceptor displacement and inner retinal change may generate metamorphopsia in MH undergoing surgery, however MH diameter is the most powerful predictor.
Is Ranking Advantage Equal to Market Power? —An Empirical Study of E-Commerce Platforms
Authors: Qu Chuang , Liu Hanzhen , Wang Sai 
Published: October 2022

Abstract
Given that consumers’ ordered search and sales are concentrated on leading merchants on Internet platforms, this paper theoretically discusses and empirically tests the relationship between ranking position and merchants’ market power on Internet platforms. Top-ranked merchants do indeed have stronger pricing power, which reflects the importance of location competition in the online market. The market power of mid-ranked merchants may be weaker than that of bottom-ranked merchants because ranking position has the function of screening consumer preferences. Under the ordered search, bottom-ranked merchants face fewer consumers, but their demand preferences are more concentrated.
Retroperitoneal lymphangioma as the final diagnosis of a middle-aged woman with abdominal pain
Authors: Aghdaei Hamid Asadzadeh , Rabbani Amirhassan , Sadeghi Amir , Rezvani Hamid , Sherkat Ghazal , Salarieh Naghmeh , Moghadam Pardis Ketabi 
Published: October 2022

Abstract
Lymphangiomas are lesions attributed to the congenital malformations of lymphatic system or acquired chronic obstruction of the lymphatic network due to trauma, radiation, surgical manipulation, inflammation or infection. Overall, lymaphangiomas are rare and particularly retroperitoneal lymphangiomas are far more uncommon per reported cases. In this study, we have introduced a middle-aged woman admitted to the gastroenterology unit with a progressive abdominal pain since approximately one month before her visit. She was found to have a retroperitoneal lymphangioma after a precise medical work up.
A novel in vitro senescent model of intervertebral disc degeneration induced by D-galactose
Authors: Zhang Shuwen , Ma Zhanbin , Sheng Weibin , Wang Hao 
Published: October 2022

Abstract
Background
Nucleus pulposus cell (NPCs) senescence within degenerative intervertebral discs is associated with the occurrence and progression of intervertebral disc degeneration (IVDD). However, there is no reliable method to induce NPCs senescence in vitro. The aim of this study was to establish a reliable and robust cell senescent model using D-galactose (D-Gal) to induce oxidative stress in NPCs.
Methods
NPCs were isolated from Sprague Dawley rats, cultured and incubated with increasing concentrations of D-Gal. Cell viability, cell cycle, senescence-associated makers and extracellular matrix were detected to evaluate the effect of D-Gal on NPCs. The migration and polarization of macrophage were observed by transwell and flow cytometry. Finally, the oxidative stress mechanism of D-Gal induced NPCs senescence was analysed by flow cytometry and absorbance analysis.
Results
Cell viability and cell cycle analyses revealed that D-Gal induced senescence by blocking DNA synthesis and decreasing NPCs proliferation. Furthermore, a dose-dependent increase in senescence-associated makers in D-Gal induced NPCs. RT-PCR analysis revealed an increase in mRNA expression of key senescence related secretory phenotype (SASP) components. Extracellular matrix was significantly decreased along with the cell senescence. Transwell and flow cytometry revealed that senescent NPC-conditioned media triggered macrophage migration and polarization. The increased of reactive oxygen species (ROS), advanced glycation end products (AGEs) and the lipid peroxidation product malondialdehyde (MDA), and decreased superoxide dismutase (SOD) activity were associated with the senescence of NPCs induced by D-Gal.
Conclusions
The results suggest that D-Gal treatment successfully induced NPCs senescence and SASP secretion. Thus, D-Gal-induced NPCs senescence is a reliable and robust cell model to study IVDD.
Ca2+ depletion in the ER causes store-operated Ca2+ entry via the TRPC6 channel in mouse brown adipocytes
Authors: Hayato Ryotaro , Matsumoto Takaya 
Published: October 2022

Abstract
Mitochondrial uncoupling by β3-adrenergic activation or an uncoupler (FCCP) causes Ca2+ release from the mitochondria and subsequent Ca2+ release from the endoplasmic reticulum (ER), evoking store-operated Ca2+ entry (SOCE) due to Ca2+ depletion from the ER in rodent brown adipocytes. In this study, we investigated how Ca2+ depletion from the ER elicits SOCE in mouse brown adipocytes using fluorometry of intracellular Ca2+ concentration ([Ca2+]i). The application of cyclopiazonic acid (CPA), a reversible sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) pump blocker in the ER, caused an increase in [Ca2+]i. Moreover, CPA-induced SOCE was suppressed by the application of a Ca2+-free Krebs solution and the transient receptor potential canonical 6 (TRPC6) blockers, which were 2-aminoethoxydiphenyl borate (2-APB), ML-9, and GsMTx-4. Application of TRPC6 channel analog 1-oleoyl-2-acetyl-sn-glycerol (OAG) and flufenamic acid elicited Ca2+ entry. Moreover, our RT-PCR analyses detected mRNAs for TRPC6, STIM1, and Orai1 in brown adipose tissues. In addition, western blot analyses showed the expression of the TRPC6 protein. Thus, TRPC6 is one of the Ca2+ pathways involved in SOCE, and Ca2+ entry is directly linked to mitochondrial uncoupling, which is involved in the late phase of β3-adrenergic or FCCP-induced [Ca2+]i increases. These modes of Ca2+ entry provide the basis for heat production via activation of Ca2+-dependent dehydrogenase and the expression of uncoupling protein 1 (UCP1) proteins. Enhancing thermogenic metabolism in brown adipocytes may serve as broad therapeutic utility to reduce obesity and metabolic syndrome.
Phospho heavy-labeled-spiketide FAIMS stepped-CV DDA (pHASED) provides real-time phosphoproteomics data to aid in cancer drug selection
Authors: Staudt Dilana E. , Murray Heather C. , Skerrett-Byrne David A. , Smith Nathan D. , Jamaluddin Muhammad F. , Kahl Richard G.S. , Duchatel Ryan J. , Germon Zacary P. , McLachlan Tabitha , Jackson Evangeline R. , Findlay Izac J. , Kearney Padraic S. , Mannan Abdul , McEwen Holly P. , Douglas Alicia M. , Nixon Brett , Verrills Nicole M. , Dun Matthew D. 
Published: October 2022

Abstract
Global high-throughput phosphoproteomic profiling is increasingly being applied to cancer specimens to identify the oncogenic signaling cascades responsible for promoting disease initiation and disease progression; pathways that are often invisible to genomics analysis. Hence, phosphoproteomic profiling has enormous potential to inform and improve individualized anti-cancer treatment strategies. However, to achieve the adequate phosphoproteomic depth and coverage necessary to identify the activated, and hence, targetable kinases responsible for driving oncogenic signaling pathways; affinity phosphopeptide enrichment techniques are required and often coupled with offline high-pressure liquid chromatographic (HPLC) separation prior to nanoflow liquid chromatography–tandem mass spectrometry (nLC-MS/MS). These complex and time-consuming procedures, limit the utility of phosphoproteomics for the analysis of individual cancer patient specimens in real-time, and restrict phosphoproteomics to specialized laboratories often outside of the clinical setting. To address these limitations, here we have optimized a new protocol, phospho-Heavy-labeled-spiketide FAIMS Stepped-CV DDA (pHASED), that employs online phosphoproteome deconvolution using high-field asymmetric waveform ion mobility spectrometry (FAIMS) and internal phosphopeptide standards to provide accurate label-free quantitation (LFQ) data in real-time. Compared with traditional single-shot LFQ phosphoproteomics workflows, pHASED provided increased phosphoproteomic depth and coverage (phosphopeptides = 4,617 pHASED, 2,789 LFQ), whilst eliminating the variability associated with offline prefractionation. pHASED was optimized using tyrosine kinase inhibitor (sorafenib) resistant isogenic FLT3-mutant acute myeloid leukemia (AML) cell line models. Bioinformatic analysis identified differential activation of the Serine/threonine protein kinase ataxia-telangiectasia mutated (ATM) pathway, responsible for sensing and repairing DNA damage in sorafenib-resistant AML cell line models, thereby uncovering a potential therapeutic opportunity. Herein, we have optimized a rapid, reproducible, and flexible protocol for the characterization of complex cancer phosphoproteomes in real-time; a step towards the implementation of phosphoproteomics in the clinic to aid in the selection of anti-cancer therapies for patients.