bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advanced oxidative decontamination of flax and its impacts on storage
Authors: Findlay Catherine , Singh Jaskirat , Nadimi Mohammad , Paliwal Jitendra 
Published: December 2022

Abstract
The metabolic actions of storage fungi and other microorganisms can cause spoilage and post-harvest losses in agricultural commodities, including flaxseed. These microbial contaminants are oxidized with hydroxyl radicals that are efficiently generated when ozone, hydrogen peroxide (H2O2), and ultraviolet (UV) light react in an advanced oxidative process (AOP). The present work explores what we believe is the first application of an AOP technology to reduce mould on whole brown and yellow flaxseed. The impact of AOP on storage and quality parameters was assessed by measuring the fatty acid value (FAV), germination rate, moisture content (MC), and visible mould growth after 12 weeks of storage. At 30°C and 75% relative humidity (RH), the yellow decontaminated flaxseed showed a 31% decrease in the number of seeds with visible mould without any adverse effect on germination rate, FAV and MC. In contrast, the same AOP treatment created an insignificant decrease in mould in stored brown flaxseed, at the cost of decreasing the germination rate and increasing FAV. Adverse effects of AOP on brown flaxseed were not readily apparent but became measurable after storage. Moreover, Fourier transform infrared (FTIR) spectroscopy was utilized to explore the rationale behind the different reactions of flaxseed varieties to AOP. The corresponding results indicated that the tolerance of yellow flaxseed to AOP might be related to its richness in olefins. The authors believe that technologies that harness advanced oxidative processes open new horizons in decontamination beyond ozone alone and towards increasing the shelf life of various agri-food products.
Quality Control Study for Piston Rings Using Pareto Analysis
Authors: Bhumarker Amit , Singh Abhishek 
Published: December 2022

Abstract
The manufacturing factor in the piston ring-shaped contact has drawn significant attention over the years, as seen by the numerous papers that have been written about it recently. The current manifestation conception addresses the requirements for a manufactured organization in order to create it successfully and efficiently, and it's more along the lines of a protective category. Modern manufacturing is extremely difficult, and it puts a firm's ability to provide in the shortest timeframe at the lowest possible cost without compromising the quality of the product being manufactured to the test. Organizations should use their resources to the greatest extent possible and reduce non-value-added tasks to the greatest extent possible in order to operate efficiently. An organization must implement all necessary measures to increase production capacity and decrease waste; as a result, critical factors like setup time, tool life, machine breakdown time, etc. must be carefully studied and monitored in order to carry out improvement actions and boost machine productivity. Tools for quality control are particularly helpful in identifying and evaluating the critical elements affecting productivity. To increase the overall production of the several piston ring manufacturing operations, a thorough investigation needed to be carried out. In Nashik, Maharashtra, India, Perfect Circles India Limited has performed the comprehensive work.
Bone marrow mesenchymal stem cell-derived exosomal microRNA-382 promotes osteogenesis in osteoblast via regulation of SLIT2
Authors: Su Hairong , Yang Yulan , Lv Wanchun , Li Xiaoli , Zhao Binxiu 
Published: December 2022

Abstract
Background:
Osteoporosis (OP) is a systemic skeletal disorder with increased bone fragility. Human bone marrow mesenchymal stem cells (hBMSCs), have multi-lineage differentiation ability, which may play important roles in osteoporosis. In this study, we aim to investigate the role of hBMSCs-derived miR-382 in osteogenic differentiation.
Methods: The miRNA and mRNA expression in peripheral blood monocytes between persons with high or low bone mineral density (BMD) were compared. Then we collected the hBMSCs-secreted sEV and examined the dominant components. The over-expression of the miR-382in MG63 cell and its progression of osteogenic differentiation were investigated by qRT-PCR, western blot and alizarin red staining. The interaction between miR-382and SLIT2 was confirmed by dual luciferase assay. The role of SLIT2 was also confirmed through up-regulation in MG63 cell, and the osteogenic differentiation-associated gene and protein were tested.
Results: According to bioinformatic analysis, a series of differential expressed genes between persons with high or low BMD were compared. After internalization of hBMSC-sEV in MG63 cells, we observed that the ability of osteogenic differentiation was significantly enhanced. Similarly, after up-regulation of miR-382 in MG63 cells, osteogenic differentiation was also promoted. According to the dual luciferase assay, the targeting function of miR-382 in SLIT2 was demonstrated. Moreover, the benefits of hBMSC-sEV in osteogenesis was abrogated through up-regulation of SLIT2.
Conclusion: Our study provided evidence that miR-382-contained hBMSC-sEV held great promise in osteogenic differentiation in MG63 cells after internalization by targeting SLIT2, which can be served as molecular targets to develop effective therapy.
Stability and Hopf bifurcation on an immunity delayed HBV/HCV model with intra- and extra-hepatic coinfection and saturation incidence
Authors: Song Bing , Zhang Yuru , Sang Yuan , Zhang Long 
Published: December 2022

Abstract
A hepatitis B or C virus (HBV or HCV) epidemic model with intra- and extra-hepatic coinfection, immune delay and saturation incidence, as well as antiviral therapy is proposed in this paper. The existence of equilibria (infection-free, immune-free and immune-activated), the basic reproduction numbers, i.e., R0, R1, are given respectively, by which the criteria on (local and global) stability of above equilibria are established. Furthermore, if the immune delay τ>τ0, the existence of Hopf bifurcation is obtained at the immune-activated equilibrium E*. Finally, the theoretical results are demonstrated by numerical simulations. We derive that the immune delay and intra- and extra-hepatic coinfection have significant influence on the transmission of HBV/HCV, could cause more complicated dynamics at E* from stability to unstablity untill bifurcation, which greatly increases the difficulty of disease control. While effective antiviral therapy could evidently decrease the spread of HBV/HCV.
Molecular phylogenetic and morphological analyses support recognition of a new species of Vincetoxicum (Apocynaceae, Asclepiadoideae) from eastern Thailand
Authors: Kidyoo Aroonrat , Kidyoo Manit 
Published: December 2022

Abstract
An unknown Vincetoxicum species has recently been discovered in northeastern Thailand. It is a twiner that resembles in its morphology and ecology V. flexuosum var. flexuosum, a variable species widely occurring from tropical Asia to Australia. Comprehensive morphological examination showed that despite similarities in growth habit, leaf shape, branching of the inflorescence and flower colour, these two taxa exhibit substantial differences in both vegetative and reproductive traits that clearly distinguish one from the other, particularly shape of the flower bud and indumentum on the corolla lobes. In addition, we also evaluated phylogenetic relationships based on DNA sequence data for ITS, trnT-L, trnL, and trnL-F markers of this new Vincetoxicum sp. with congeners (including, inter alia, new sequences of V. flexuosum var. flexuosum and also those of V. flexuosum var. tenuis, the other variety occurring in Thailand). The analyses demonstrated that the new Vincetoxicum sp. is not closely related to the taxa recognized as V. flexuosum. The new species was instead retrieved as sister to a clade containing the African taxa, V. caffrum, V. lycioides and V. fleckii. Therefore, integrated analyses of morphology and molecular phylogeny revealed Vincetoxicum sp. to be a well-defined species clearly distinct from V. flexuosum, as well as from all other known congeners. The morphological similarity between the new Vincetoxicum sp. and V. flexuosum var. flexuosum likely resulted from convergence, leading to various taxonomic complications. We here describe it as a new species, V. sangyojarniae, sp. nov., and provide a detailed description, illustration, photographs, and comparison to the morphologically similar V. flexuosum var. flexuosum. A preliminary taxonomic reconsideration of the infraspecific taxa under V. flexuosum is also suggested.
A Data-driven Analysis and Optimization of the Impact of Prescribed Fire Programs on Wildfire Risk in Different Regions of the United States
Authors: Jose Esther , Agarwal Puneet , Zhuang Jun 
Published: December 2022

Abstract
In the current century, wildfires have shown an increasing trend, causing a huge amount of direct and indirect losses in society. Different methods and efforts have been employed to reduce the frequency and intensity of the damages, one of which is implementing prescribed fires. Previous works have established that prescribed fires are effective at reducing the damage caused by wildfires. However, the actual impact of prescribed fire programs are dependent on factors such as where and when prescribed fires are conducted. In this paper, we propose a novel data-driven model studying the impact of prescribed fire as a mitigation technique for wildfires to minimize the total costs and losses. This is applied to states in the U.S. to perform a comparative analysis of the impact of prescribed fires from 2003-2017 and to identify the optimal scale of the impactful prescribed fire programs. The fifty U.S. states are classified into categories based on impact and risk levels. Measures that could be taken to improve different prescribed fire programs are discussed. Our results show that California and Oregon are the only severe-risk U.S. states to conduct prescribed fire programs that are impactful at reducing wildfire risks, while other southeastern states such as Florida maintain fire-healthy ecosystems with very extensive prescribed fire programs. Our research provides a novel model and insights for wildfire management.
Comparison of the results of the modified and classical bimedial recession in infantile esotropia
Authors: BEĞENDİ Diğdem , DURANOĞLU Yaşar 
Published: December 2022

Abstract
Purpose
Bi-medial rectus recession, which can also be performed using a hang-back technique, is one of the surgical treatment options for infantile esotropia (IE). This study has modified the surgical approach, with outcomes compared to the traditional hang-back technique.
Methods
The bi-medial recession was performed with a modified hang-back technique in 120 IE patients and with a traditional hang-back technique in 88 cases. Surgical outcomes were reviewed and compared retrospectively.
Results
The patients in the two groups were compared in terms of surgery time, inferior oblique weakening surgery, and the presence of refractive error. The differences between pre-operative deviation degrees and post-operative first-month, sixth-month, and first-year deviation degrees were all statistically significant (p < 0.001).
Conclusions
This modified novel technique aims to avoid unwanted muscle movement in the horizontal and vertical axes and a gap in the middle of the recessed muscle, as seen in the traditional hang-back technique. Further, the modified technique resulted in less over- and under-correction as well as alphabetic pattern deviation.
Comparison of in vitro Antifungal Activity Methods Using Aeromonas sp. BHC02 Chitinase, Whose Physicochemical Properties were Determined as Antifungal Agent Candidate
Authors: Yilmaz Gulesme , CADIRCI Bilge 
Published: December 2022

Abstract
Biological control to prevent fungal plant diseases offers and alternative approach to faciliate sustainable agriculture. Since inhibition of chitinolytic fungal cell walls synthesis is a target for antifungal agents, chitinases are one of the biocontrol agents. This study, it was aimed to investigate isolating a new bacterium from fluvial soil as a chitinase source and the antifungal activity of the characterized chitinase. During planning the in vitro antifungal activity, three common methods were preferred and compared. The bacterium with the highest chitinase activity was identified as Aeromanas caviae by 16S rRNA sequence analysis. Following the determination of the optimum enzyme production time, the enzyme was partially purified, and the physicochemical parameters of the enzyme were investigated. It was determined that the partially purified chitinase showed antifungal activity against Alternaria alternata, Fusarium solani, Botrytis cinerea, Penicillium sp. This study also conclude that the results of the antifungal activities depend on the method used. And all fungal chitins cannot be degraded with a chitinase. Depending on the variety of chitin, some fungi can be more resistant. In this context, it is necessary to conduct a detailed study on the chitins in the cell wall of the fungi.
A Methodology Framework for Bipartite Network Modeling
Authors: Liew Chin Ying , Labadin Jane , Kok Woon Chee , Eze Monday Okpoto 
Published: December 2022

Abstract

The graph-theoretic based studies employing bipartite network approach mostly focus on surveying the statistical properties of the structure and behavior of the network systems under the domain of complex network analysis. They aim to provide the big-picture-view insights of a networked system by looking into the dynamic interaction and relationship among the vertices. Nonetheless, incorporating the features of individual vertex and capturing the dynamic interaction of the heterogeneous local rules governing each of them in the studies is lacking. The methodology in achieving this could hardly be found. Consequently, this study intends to propose a methodology framework that considers the inﬂuence of heterogeneous features of each node to the overall network behavior in modeling real-world bipartite network system. The proposed framework consists of three main stages with principal processes detailed in each stage, and three libraries of techniques to guide the modeling activities. It is iterative and process-oriented in nature and allows future network expansion. Two case studies from the domain of communicable disease in epidemiology and habitat suitability in ecology employing this framework are also presented. The results obtained suggest that the methodology could serve as a generic framework in advancing the current state of the art of bipartite network approach.
Development of an Educational Program in Rational Drug Use by Primary Care Pharmacists toward Village Health Volunteers’ Literacy: A Preliminary Study in Rural Northern Areas of Thailand
Authors: Lertphanithit Sunthiwat , Panya Rujira , Maneemai Ong-art 
Published: December 2022

Abstract
Adequate rational drug use (RDU) literacy of village health volunteers (VHVs) is essential for community health. This study aimed to develop and examine the effects of educational program by primary care pharmacist on RDU literacy among VHVs. This study was one-group pre-test-and-post-test study carried out at Sawang Arom Health Promotion Hospital, Dok Khamtai district, Phayao province, Thailand. The educational program was designed based on health literacy theory. The primary outcome was RDU literacy score. The Rational Drug Use Literacy Scale was used to assess the RDU literacy score. Sixty VHVs were recruited into the study via purposive sampling. The results demonstrated that most of participants were female (n=54, 90.0%). Approximately 70% (n=43) were over the age of 50, and 80% (n=48) were had educational level under grade 9. The mean RDU literacy scores in pre-test and post-test were 18.85 ± 5.25 and 23.93 ± 2.15, respectively (p<0.001). Considering by aspects, it was found that the mean scores in compliance to the instructions on a medicine label (p<0.001), health advertising assessment (p<0.001), proper drug selection and utilization (p<0.001), and medical term comprehension (p=0.006) were statistical significantly higher than pre-test. Health information accessibility score was not significantly different from the baseline (p=0.766). In conclusion, this pharmacist-developed educational program demonstrated effectively improved RDU literacy among VHVs. It may be advantageous to use this program in other areas.
Comparing RNA extraction methods to face the variations in RNA quality using two human biological matrices
Authors: Ortega-Pinazo Jesús , Serrano-Castro Pedro Jesús , Martínez Beatriz , Pinto-Medel María Jesús , Gómez-Zumaquero Juan Miguel , Lago-Sampedro Ana , García-Díaz Beatriz , Estivill-Torrús Guillermo , Ferro Pedro 
Published: December 2022

Abstract
Nucleic acids, RNA among them, are widely used in biomedicine and biotechnology. Because of their susceptibility to degradation by RNases, the handling and extraction process of RNA from cells and tissues require specialized personnel and standardized methods to guarantee high purity and integrity. Due to the diversity of techniques found in the market, a comparative study between different RNA extraction methods is useful to facilitate the best choice for the researcher or in research service platforms such as biobanks to see the traceability of the samples. In this study, we have compared seven different RNA extraction methods: manual (TRIzol™), semiautomated (QIAGEN™, Bio-Rad, Monarch®, and Canvax™), and fully automated (QIAcube™ and Maxwell®) processes, from two biological matrices: human Jurkat T cells and peripheral blood mononuclear cells (PBMC). Results showed marked differences in the RNA quality and functionality according to the method employed for RNA extraction and the matrix used. These data contribute to facilitating researchers or research service platforms in decision-making practices and emphasize the relevance of the selection of the RNA extraction method in each experimental procedure or traceability study to guarantee both quality standards and its reproducibility.
Niobium pentaoxide (Nb2O5) as an efficient photocatalyst for photocatalytic degradation of rhodamine-B
Authors: Topare Niraj S. , Khan Anish , Khedkar Satish V. , Bokil Shantini A. 
Published: December 2022

Abstract
One of the most important problems that the world is currently facing in terms of its impact on the environment is figuring out how to properly manage textile effluents. Photocatalysis has been shown to be successful in the removal of intractable chemicals and is regarded as a viable wastewater treatment technology. This work focused on the photocatalytic degradation of rhodamine-B (RB) in an aqueous solution, as well as the photocatalytic behavior of niobium pentaoxide (Nb2O5) as a photocatalyst was tested. It is important to note that good photocatalytic efficiency is highly dependent on the operating conditions. There are several process parameters that influence RB photocatalytic degradation, including the amount of photocatalyst (Nb2O5) used, the concentration of RB at the start of the reaction, and the pH of the solution optimized under visible-light irradiation. According to the findings, the conditions in which the greatest amount of RB was degraded were those in which the concentration of the catalyst was 10 mg/l, the dosage of the catalyst was 1 mg/l, and the pH was 11. The results also revealed that after utilizing the catalyst three times in a row, catalyst efficiency was maintained, and the degradation rate was maintained at a greater level.
Anammox process for aquaculture wastewater treatment: operational condition, mechanism, and future prospective
Authors: Ismail Ismafatin Nabilah , Taufik Muhammad , Umor N. A. , Norulhuda Mohamed Ramli , Zulkarnaini Zulkarnaini , Ismail Shahrul 
Published: December 2022

AbstractTreatment of ammonia- and nitrate-rich wastewater, such as that generated in the aquaculture industry, is important to prevent environmental pollution. The anaerobic ammonium oxidation (anammox) process has been reported as a great alternative in reducing ammoniacal nitrogen concentration in aquaculture wastewater treatment compared to conventional treatment systems. This paper will highlight the impact of the anammox process on aquaculture wastewater, particularly in the regulation of ammonia and nitrogen compounds. The state of the art for anammox treatment systems is discussed in comparison to other available treatment methods. While the anammox process is viable for the treatment of aquaculture wastewater, the efficiency of nitrogen removal could be further improved through the proper use of anammox bacteria, operating conditions, and microbial diversity. In conclusion, a new model of the anammox process is proposed in this review.
Electron beam treatment for the removal of 1,4-dioxane in water and wastewater
Authors: Pearce Robert , Li Xi , Vennekate John , Ciovati Gianluigi , Bott Charles 
Published: December 2022

AbstractElectron beam (e-beam) treatment uses accelerated electrons to form oxidizing and reducing radicals when applied to water without the use of external chemicals. In this study, electron beam treatment was used to degrade 1,4-dioxane in several water matrices. Removal improved in the progressively cleaner water matrices and removals as high as 94% to 99% were observed at a dose of 2.3 kGy in secondary effluent. 1,4-dioxane removal was confirmed to be primarily through hydroxyl radical oxidation. The calculated electrical energy per order was found to be 0.53, 0.26, and 0.08 kWh/m3/order for secondary effluent (Avg. total organic carbon (TOC) 9.25 mg/L), granular activated carbon effluent (TOC 3.46 mg/L), and ultrapure water, respectively, with a 70% generation and transfer efficiency applied.
Calcium and Sodium-mediated Dynamic Assembly of Intermediate Filament-like Protein FilP
Authors: Chen Ximing , Sun Xiuxia , Fu Caihong , Zhang Shanshan , Yang Fan 
Published: December 2022

Background:Cytoskeletal elements play key roles in cell morphology, cell division, cell mobility, and DNA partitioning in all domains of life. The IF-like protein FilP was discovered in Streptomyces coelicolor, and it was found to perform a structurally important cytoskeletal role by providing direct mechanical support for the cells.Objective:This work investigated the factors influencing FilP polymerization under a variety of conditions.Methods:DLS technique was applied to real-time monitor the in vitro assembly process of Streptomyces coelicolor FilP.Results:The presence of small amounts of divalent cations, such as CaCl2 or MgCl2, enhanced the polymerization of FilP, while higher amounts suppressed its polymerization. Moreover, high concentrations of NaCl, KCl, NH4Cl, and KNO3 both suppressed the polymerization of FilP. EDTA was found to have a very prohibitive effect on FilP polymerization, and even the following addition of Ca2+ could not initiate the assembly of FilP. FilP polymerized under a range of pHs ranging from pH 6 to pH 8, while the polymerization degree was sensitive to pH. FilP formed network-like, striated filaments at neutral pH, while the filaments became more disordered or loosely packed at pH 8 and pH 6, respectively.Conclusion:FilP assembly is calcium-mediated. Ca2+ is not only required for FilP polymerization, but also required for FilP to maintain the higher-order polymer structure. The accelerative effect of Ca2+ and the suppressive effect of Na+ persisted under a wide range of conditions, suggesting that FilP might use calcium and sodium ions as a general mechanism to mediate its polymerization process.