bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low Cost Synthesis and Characterization of Donor P3HT Polymer for Fabrication of Organic Solar Cell
Authors: D S Suresh , Vandana M , Veeresh S , Ganesh H , Nagaraju Y S , Vijeth H , Basappa M , Devendrappa H 
Published: April 2022

Abstract
Over the years, the conjugated polymers acts as electron donor material in the active layer of Organic solar cells (OSCs). By altering means of doping, prepare a composite with suitable materials from Poly (p-phenylene vinylene) PPV polymer toPM6 polymer which gives Power conversion efficiency (PCE) can vary from ~ 2% to ~15%. But these are expensive, complicated synthetic procedure and less availability. To overcome this,the conjugated polymer Poly (3-Hexyl Thiophene)(P3HT) synthesized through oxidative coupling of 3-HT monomer and Ferric chloride (FeCl3) as an oxidant which is low cost, available and very easy method of synthesis. We investigated synthesized P3HT material that shows the Fourier Transform Infrared(FTIR) peaks shows that intramolecular interactions from thiophene ring, alkyl chain and polymer chain, FESEM Images reveals morphology consists of condensed layers in micrometer dimensions and Cyclic voltammetry studies gives oxidation and reduction potentials to promote the higher ionization potential of the compound to use as donor material in OSCs.
The optimized combination of aCompCor and ICA-AROMA to reduce motion and physiologic noise in task fMRI data
Authors: Van Schuerbeek P , De Wandel L , Baeken C 
Published: April 2022

Abstract
One of the main challenges in fMRI processing is filtering the task BOLD signals from the noise. Independent component analysis with automatic removal of motion artifacts (ICA-AROMA) reduces motion artifacts by identifying ICA noise components based on their location at the brain edges and cerebrospinal fluid (CSF), high frequency content and correlation with motion regressors. In anatomical component correction (aCompCor), physiological noise regressors extracted from CSF were regressed out from the fMRI time series. In this study, we compared three methods to combine aCompCor and ICA-AROMA denoising in one denoising step. In the first analysis, we regressed the temporal signals of the ICA components identified as noise by ICA-AROMA together with the noise signals determined by aCompCor from the fMRI signals. For the second and third analyses, the correlation between the temporal signals of the ICA components and the aCompCor noise signals was used as an additional criterion to identify the noise components. In the second analysis, the temporal signals of the ICA components classified as noise were regressed from the fMRI signals. In the third analysis, the noise components were removed. To compare the denoising strategies, we examined the fractional amplitude of low-frequency fluctuations (fALFF) and the overlap between the contrast maps. Our results revealed that including the aCompCor noise signals as regressors in ICA-AROMA resulted in more correctly identified noise components, higher fALFF values, and larger activation maps. Moreover, combining the temporal signals of the noise components identified by ICA-AROMA with the aCompCor signals in a noise regression matrix resulted in deactivations. These results suggest that using the correlation between the ICA component temporal signals and the aCompCor signals as noise identification criteria in ICA-AROMA is the best approach for combining both denoising methods.
Fabrication methods of glass fibre composites—a review
Authors: Kulhan Taniya , Kamboj Arun , Gupta Nitin Kumar , Somani Nalin 
Published: April 2022

AbstractThe use of polymer composite has been implemented since 3400 B.C, the very first known composite’s application is attributed to the Mesopotamians. These ancient people fabricated plywood with glued wood strips placed at various distinct angles and in the late 1930s glass fiber thin strands have been developed. Glass fibre polymer composites have a wide scope in various engineering structures submarines, spacecraft, airplanes, automobiles, sports, and many more, over traditional materials because of their superior properties including lightweight, high fracture toughness, corrosion, fatigue, wear & fire resistance, high strength to weight ratio, high modulus and low coefficient of expansion. Various technologies have been developed so far to create different types of polymer composites in accordance with their properties and applications. Glass fiber possesses better properties as great strength, better flexibility, stiffness, and chemical corrosion resistance. Glass fibers are generally in the form of cut-up strand, fabrics and mats. Every kind of glass fibers has different properties and has various applications as in polymer composites. The aim of this review paper is to provide updated technological insights regarding the evolution of composite, classification of gass fibre polymer composites, development methodology in contrast with various applications, advantages and limitations and their behavioral properties.
Lifting the Mouth Corner: A Systematic Review of Techniques, Clinical Outcomes, and Patient Satisfaction
Authors: van der Sluis Nanouk , Gülbitti Haydar A , van Dongen Joris A , van der Lei Berend 
Published: April 2022

Abstract

Background
Mouth corners are an essential part of the centrofacial area for perception of attractiveness and emotions. Downturned mouth corners are a result of aging or have a congenital origin. Different mouth corner lifting techniques are described in the literature.

Objectives
This review was performed to systematically assess and compare invasive and noninvasive mouth corner lifting techniques and their effectiveness, patient satisfaction, and adverse effects.

Methods
MEDLINE (via PubMed), EMBASE (OvidSP), and the Cochrane Central Register of controlled trials databases were searched for clinical and observational studies published in peer-reviewed academic journals with abstracts available (searched from May 18, 2019, to December 18, 2021). Outcomes of interest were aesthetic mouth corner lifting techniques, the degree of lift as well as the longevity of the lifting effect, patient satisfaction, and adverse effects. Techniques were subdivided in invasive techniques and noninvasive techniques.

Results
Out of 968 studies found from the search, 11 were included in the qualitative analysis. In general, surgical techniques seem to have a better mouth corner lifting effect than nonsurgical techniques; however, objective evidence is weak, and surgery inevitably results in a scar. Reported patient satisfaction was good for both surgical and nonsurgical techniques and no severe complications have been described.

Conclusions
Surgical techniques seem to have a better lifting effect on mouth corners than nonsurgical techniques. Nevertheless, objective evidence is weak, and a scar is inevitable.

Level of Evidence: 4
Paternal obesity induces placental hypoxia and sex-specific impairments in placental vascularization and offspring metabolism
Authors: Jazwiec Patrycja A , Patterson Violet S , Ribeiro Tatiane A , Yeo Erica , Kennedy Katherine M , Mathias Paulo C F , Petrik Jim J , Sloboda Deborah M 
Published: April 2022

Abstract
Paternal obesity predisposes offspring to metabolic dysfunction, but the underlying mechanisms remain unclear. We investigated whether this metabolic dysfunction is associated with changes in placental vascular development and is fueled by endoplasmic reticulum (ER) stress-mediated changes in fetal hepatic development. We also determined whether paternal obesity indirectly affects the in utero environment by disrupting maternal metabolic adaptations to pregnancy. Male mice fed a standard chow or high fat diet (60%kcal fat) for 8–10 weeks were time-mated with female mice to generate pregnancies and offspring. Glucose tolerance was evaluated in dams at mid-gestation (embryonic day (E) 14.5) and late gestation (E18.5). Hypoxia, angiogenesis, endocrine function, macronutrient transport, and ER stress markers were evaluated in E14.5 and E18.5 placentae and/or fetal livers. Maternal glucose tolerance was assessed at E14.5 and E18.5. Metabolic parameters were assessed in offspring at ~60 days of age. Paternal obesity did not alter maternal glucose tolerance but induced placental hypoxia and altered placental angiogenic markers, with the most pronounced effects in female placentae. Paternal obesity increased ER stress-related protein levels (ATF6 and PERK) in the fetal liver and altered hepatic expression of gluconeogenic factors at E18.5. Offspring of obese fathers were glucose intolerant and had impaired whole-body energy metabolism, with more pronounced effects in female offspring. Metabolic deficits in offspring due to paternal obesity may be mediated by sex-specific changes in placental vessel structure and integrity that contribute to placental hypoxia and may lead to poor fetal oxygenation and impairments in fetal metabolic signaling pathways in the liver.
The ectodysplasin-A receptor is a candidate gene for lateral plate number variation in stickleback fish
Authors: Laurentino Telma G , Boileau Nicolas , Ronco Fabrizia , Berner Daniel 
Published: April 2022

AbstractVariation in lateral plating in stickleback fish represents a classical example of rapid and parallel adaptation in morphology. The underlying genetic architecture involves polymorphism at the ectodysplasin-A gene (EDA). However, lateral plate number is influenced by additional loci that remain poorly characterized. Here, we search for such loci by performing genome-wide differentiation mapping based on pooled whole-genome sequence data from a European stickleback population variable in the extent of lateral plating, while tightly controlling for the phenotypic effect of EDA. This suggests a new candidate locus, the EDA receptor gene (EDAR), for which additional support is obtained by individual-level targeted Sanger sequencing and by comparing allele frequencies among natural populations. Overall, our study illustrates the power of pooled whole-genome sequencing for searching phenotypically relevant loci and opens opportunities for exploring the population genetics and ecological significance of a new candidate locus for stickleback armor evolution.
A rare frameshift mutation inSYCP1is associated with human male infertility
Authors: Nabi Soheila , Askari Masomeh , Rezaei-Gazik Maryam , Salehi Najmeh , Almadani Navid , Tahamtani Yaser , Totonchi Mehdi 
Published: April 2022

AbstractProper assembly of the synaptonemal complex is essential for successful meiosis, and impairments in the process lead to infertility. Meiotic transverse filament proteins encoded by the SYCP1 (synaptonemal complex protein 1) gene are one of the main components of the synaptonemal complex and play an important role in correct synapsis and recombination. Family-based whole-exome sequencing revealed a rare homozygous SYCP1 frameshift mutation (c.2892delA: p.K967Nfs*1) in two men with severe oligozoospermia, followed by validation and segregation through Sanger sequencing. This single nucleotide deletion not only changes lysine 967 (K) into asparagine (N) but also causes a premature stop codon, which leads to deletion of 968–976 residues from the end of the C-tail region of the SYCP1 protein. Although, sycp1 knockout male mice are reported to be sterile with a complete lack of spermatids and spermatozoa, to date no SYCP1 variant has been associated with human oligozoospermia. HADDOCK analysis indicated that this mutation decreases the ability of the truncated SYCP1 protein to bind DNA. Immunodetection of ϒH2AX signals in SYCP1 mutant semen cells, and a 40% DNA fragmentation index might indicate that a small number of DNA double-strand breaks, which require SYCP1 and/or synapsis to be repaired, are not efficiently repaired, resulting in defects in differentiation of germline cells and appearance of the oligozoospermia phenotype. To our knowledge, this is the first report of a homozygous SYCP1 mutation that decreases sperm count. Further studies are required to determine the function of the SYCP1 mutation, which is potentially associated with human oligozoospermia.
Partially functional NARROW LEAF1 balances leaf photosynthesis and plant architecture for greater rice yield
Authors: Ouyang Xiang , Zhong Xiaoyu , Chang Shuoqi , Qian Qian , Zhang Yuzhu , Zhu Xinguang 
Published: April 2022

Abstract
NARROW LEAF1 (NAL1) is an elite gene in rice (Oryza sativa), given its close connection to leaf photosynthesis, hybrid vigor, and yield-related agronomic traits; however, the underlying mechanism by which this gene affects these traits remains elusive. In this study, we systematically measured leaf photosynthetic parameters, leaf anatomical parameters, architectural parameters, and agronomic traits in indica cultivar 9311, in 9311 with the native NAL1 replaced by the Nipponbare NAL1 (9311-NIL), and in 9311 with the NAL1 fully mutated (9311-nal1). Leaf length, width, and spikelet number gradually increased from lowest to highest in 9311-nal1, 9311, and 9311-NIL. In contrast, the leaf photosynthetic rate on a leaf area basis, leaf thickness, and panicle number gradually decreased from highest to lowest in 9311-nal1, 9311, and 9311-NIL. RNA-seq analysis showed that NAL1 negatively regulates the expression of photosynthesis-related genes; NAL1 also influenced expression of many genes related to phytohormone signaling, as also shown by different leaf contents of 3-Indoleacetic acid, jasmonic acid, Gibberellin A3, and isopentenyladenine among these genotypes. Furthermore, field experiments with different planting densities showed that 9311 had a larger biomass and yield advantage under low planting density compared to either 9311-NIL or 9311-nall. This study shows both direct and indirect effects of NAL1 on leaf photosynthesis; furthermore, we show that a partially functional NAL1 allele helps maintain a balanced leaf photosynthesis and plant architecture for increased biomass and grain yield in the field.
13C-labeling reveals how membrane lipid components contribute to triacylglycerol accumulation in Chlamydomonas
Authors: Young Danielle Yvonne , Pang Na , Shachar-Hill Yair 
Published: April 2022

Abstract
Lipid metabolism in microalgae has attracted much interest due to potential utilization of lipids as feedstocks for biofuels, nutraceuticals, and other high-value compounds. Chlamydomonas reinhardtii is a model organism for characterizing the synthesis of the neutral lipid triacylglycerol (TAG), from which biodiesel is made. While much of TAG accumulation under N-deprivation is the result of de novo fatty acid (FA) synthesis, recent work has revealed that approximately one-third of FAs, especially polyunsaturated FAs (PUFAs), come from preexisting membrane lipids. Here, we used 13C-isotopic labeling and mass spectrometry to analyze the turnover of glycerol backbones, headgroups, FAs, whole molecules, and molecular fragments of individual lipids. About one-third of the glyceryl backbones in TAG are derived from preexisting membrane lipids, as are approximately one-third of FAs. The different moieties of the major galactolipids turn over synchronously, while the FAs of diacylglyceryltrimethylhomoserine (DGTS), the most abundant extraplastidial lipid, turn over independently of the rest of the molecule. The major plastidic lipid monogalactosyldiacylglycerol (MGDG), whose predominant species is 18:3α/16:4, was previously shown to be a major source of PUFAs for TAG synthesis. This study reveals that MGDG turns over as whole molecules, the 18:3α/16:4 species is present in both DAG and TAG, and the positional distribution of these PUFAs is identical in MGDG, DAG, and TAG. We conclude that headgroup removal with subsequent acylation is the mechanism by which the major MGDG species is converted to TAG during N-deprivation. This has noteworthy implications for engineering the composition of microalgal TAG for food, fuel, and other applications.
Development of the Warwick Axial Spondyloarthritis faTigue and Energy questionnaire (WASTEd)—a new patient-reported outcome measure
Authors: Pearson Nathan A , Tutton Elizabeth , Martindale Jane , Strickland George , Thompson Jean , Packham Jonathan C , Creamer Paul , Haywood Kirstie L 
Published: April 2022

Abstract

Objective
The aim was to co-produce and test a potential new patient-reported outcome measure (PROM), the Warwick Axial Spondyloarthritis faTigue and Energy questionnaire (WASTEd), providing vital qualitative confirmation of conceptual relevance, clarity and acceptability.

Methods
Informed by measurement theory, we collaborated with patient partners throughout a three-stage, iterative process of PROM development. In stage 1, informed by patient interviews, reviews exploring patients’ fatigue experiences and existing PROMs of fatigue, an initial measurement framework of axial spondyloarthritis (axSpA) fatigue and energy and candidate items were defined. In stage 2, the relevance and acceptability of the measurement framework and candidate items were assessed qualitatively by focus group participants. In stage 3, patients participated in pre-testing interviews to assess item comprehensiveness, relevance, acceptability and comprehensibility.

Results
Stage 1 informed the development of an initial five-domain measurement framework with 59 candidate items. In stage 2, five patients and seven health-care professionals participated in four focus groups to derive a 40-item model of fatigue and energy. Collaborative engagement with patient research partners supported refinement of questionnaire structure and content further. Pre-testing with ten patients across two interview rounds in stage 3 produced a four-domain, 30-item long-form questionnaire.

Conclusion
An active collaboration with patients and health-care professionals has supported the co-production of a potential new PROM of axSpA fatigue, underpinned by strong evidence of face and content validity. The WASTEd extends the assessment of fatigue beyond severity, highlighting the importance of symptom frequency, energy and self-management. Future research will involve psychometric evaluation, supporting item reduction, structural refinement and confirmation of PROM validity.
Safety and efficacy of filgotinib, lanraplenib and tirabrutinib in Sjögren’s syndrome: a randomized, phase 2, double-blind, placebo-controlled study
Authors: Price Elizabeth , Bombardieri Michele , Kivitz Alan , Matzkies Franziska , Gurtovaya Oksana  , Pechonkina Alena , Jiang Wendy , Downie Bryan , Mathur Anubhav , Mozaffarian Afsaneh , Mozaffarian Neelufar , Gottenberg J Eric 
Published: April 2022

Abstract

Objective
The aim of this study was to characterize the safety and efficacy of filgotinib, lanraplenib and tirabrutinib in patients with active SS.

Methods
This multicentre, double-blind study randomized patients with active primary or secondary SS [EULAR SS disease activity index (ESSDAI) ≥5) to receive filgotinib 200 mg (Janus kinase-1 inhibitor), lanraplenib 30 mg (spleen tyrosine kinase inhibitor), tirabrutinib 40 mg (Bruton’s tyrosine kinase inhibitor), or placebo. The composite primary end point was the week-12 proportion of patients fulfilling protocol-specified improvement criteria (based on CRP and SS-related symptoms). The EULAR SS patient-reported index (ESSPRI) and the ESSDAI change from baseline (CFB) were secondary end points. Exploratory end points included disease-related biomarkers. Treatment-emergent adverse events (AEs) represented safety outcomes.

Results
The mean of the baseline ESSDAI was 10.1, and of ESSPRI was 6.2 in the 150 patients who were treated; 125 completed the 24-week placebo-controlled treatment period. At week 12, 43.3% of the filgotinib group achieved the primary end point (P = 0.17 vs placebo) vs 42.3% (P = 0.16), 34.7% (P = 0.33), and 26.7% of lanraplenib, tirabrutinib, and placebo groups, respectively. Neither secondary end point was met. Biomarker reductions included immunoglobulins classically associated with SS disease activity. Filgotinib ESSDAI CFB appeared more pronounced in subgroups with baseline ESSDAI ≥14 or without DMARDs/CSs. Most AEs were Grade 1 or 2.

Conclusion
Three drugs with disparate mechanisms were tested, but no significant differences vs placebo in primary or secondary end points were observed. These results may be considered hypothesis-generating, given the drug tolerability, subgroup analysis, and biomarker findings.

Trial registration
ClinicalTrials.gov, https://clinicaltrials.gov, NCT03100942.
Evolutionary analyses of emerging GII.2[P16] and GII.4 Sydney [P16] noroviruses
Authors: Zheng Guo-li , Zhu Zheng-xi , Cui Jia-le , Yu Jie-mei 
Published: April 2022

Abstract
GII.2[P16] and GII.4 Sydney [P16] are currently the two predominant norovirus genotypes. This study sought to clarify their evolutionary patterns by analyzing the major capsid VP1 and RNA-dependent RNA polymerase (RdRp) genes. Sequence diversities were analyzed at both nucleotide and amino acid levels. Selective pressures were evaluated with the Hyphy package in different models. Phylogenetic trees were constructed by the maximum likelihood method from full VP1 sequences, and evolutionary rates were estimated by the Bayesian Markov Chain Monte Carlo approach. The results showed that (1) several groups of tightly linked mutations between the RdRp and VP1 genes were detected in the GII.2[P16] and GII.4[P16] noroviruses, and most of these mutations were synonymous, which may lead to a better viral fitness to the host; (2) although the pattern of having new GII.4 variants every 2–4 years has been broken, both the pre- and the post-2015 Sydney VP1 had comparable evolutionary rates to previously epidemic GII.4 variants, and half of the major antigenic sites on GII.4 Sydney had residue substitutions and several caused obvious changes in the carbohydrate-binding surface that may potentially alter the property of the virus; and (3) GII.4 Sydney variants during 2018–21 showed geographical specificity in East Asia, South Asia, and North America; the antigenic sites of GII.2 are strictly conserved, but the GII.2 VP1 chronologically evolved into nine different sublineages over time, with sublineage IX being the most prevalent one since 2018. This study suggested that both VP1 and RdRp of the GII.2[P16] and GII.4 Sydney [P16] noroviruses exhibited different evolutionary directions. GII.4[P16] is likely to generate potential novel epidemic variants by accumulating mutations in the P2 domain, similar to previously epidemic GII.4 variants, while GII.2[P16] has conserved predicted antigenicity and may evolve by changing the properties of nonstructural proteins, such as polymerase replicational fidelity and efficiency. This study expands the understanding of the evolutionary dynamics of GII.2[P16] and GII.4[P16] noroviruses and may predict the emergence of new variants.