bluuebunny/crossref_metadata_2025_split huggingface dataset:
EU Public Legitimation in the Social Media Era: Co‐ordinating the Political Communication of the European Commission
Authors: Aagaard Peter 
Published: August 2022

AbstractThis article addresses the question of how the EU gains influence over public legitimacy in the social media era. Existing theories focus either on the type of legitimacy at play without addressing strategic efforts in the digital public sphere, or they focus on self‐legitimation without considering the type of legitimacy at stake. However, public legitimation is the result of both institutional conditions and of agency. Strategic efforts in public legitimation are shaped not only by choices between forms of co‐ordination but also by the type of legitimacy these efforts attempt to influence. Concurrently, the purpose here is to suggest a new approach to analysing the EU's responses to public legitimacy challenges and to detecting features of good practice in the digital public sphere. Taking a historical approach, the article links existing literatures addressing EU legitimacy, organizational strategy, and political communication. The European Commission is used as an illustrative case example.
Establishment of a cell line derived from the gills ofGymnocypris przewalskii, an endemic Schizothoracine fish from Qinghai Lake of Tibet Plateau
Authors: Wei Fulei , Liang Jian , Yue Miao , Tian Wengen , Yu Luxian , Feng Zhaohui , Hua Qiang 
Published: August 2022

AbstractGymnocypris przewalskii(Naked carp), a native teleost, plays an important role in maintaining the ecological balance of Lake Qinghai (altitude, 3.2 km), the largest saline lake in China. In this study, a new gill cell line fromG. przewalskiiwas developed using the explant technique and named as GPG. This cell line was maintained in Dulbecco's Modified Eagle Medium (DMEM) (high glucose), supplemented with 15% fetal bovine serum (FBS), and was successfully subcultured up to 32 passages. Meanwhile, this cell line was also authenticated by sequencing themitochondrial cytochrome C oxidase subunit I(COI) and16S rRNAgenes and by chromosome analysis. With the Cytomegalovirus (CMV) promoter, the GPG cell line could express green fluorescent protein (GFP) at about 5% transfection efficiency. MTT test showed that Clostridium botulinum toxin (BTX) was toxic to the cell line. After cryopreservation with 10% dimethyl sulfoxide (DMSO), this cell line could be successfully revived at an efficiency over 70%. This study revealed that the GPG cell line could be used as materials for physio‐chemical investigation ofG. przewalskiiand also provided a tool for gene function study and toxicological reactionin vitro.
Phenotypic and genotypic analysis of Edwardsiella isolates from Taiwan indicates wide variation with a particular reference to Edwardsiella tarda and Edwardsiella anguillarum
Authors: Rahmawaty Atiek , Chen Mei‐Yun , Byadgi Omkar Vijay , Wang Pei‐Chi , Chen Shih‐Chu 
Published: August 2022

AbstractEdwardsiella spp. is a gram‐negative, facultatively anaerobic, intracellular bacteria threatening the aquaculture industry worldwide. Noticeably, E. tarda is now genotypically classified into three distinct groups (E. tarda, E. piscicida and E. anguillarum), but morphologically, it is unclear due to varying degrees of virulence in different fish hosts. Hence, to reclassify E. tarda, we investigated differences in genotypes, phenotypes and pathogenicity. We collected Edwardsiella isolates from five different counties of Taiwan between 2017 and 2021. At first, gyrB gene was amplified for a phylogenetic tree from 40 isolates from different fish and one reference isolate, BCRC10670, from the human. Thirty‐nine strains clustered into E. anguillarum, 1 strain into E. piscicida and 1 strain into E. tarda from human strain. Second, all isolates were characterized using various phenotypic (API 20E biochemical profiles) and genotypic (pulsed‐field gel electrophoresis [PFGE], and virulence‐related gene detection). SpeI digestion revealed 10 pulsotypes and I‐CeuI into 7 pulsotypes. Virulent genes (citC, gadB, katB, mukF and fimA) confirmed in 35, 31, 28, 37 and 38 isolates, respectively. Finally, in vivo challenge test in milkfish (Chanos chanos) indicated the highest mortality from E. anguillarum. Overall, results revealed unique features with Edwardsiella spp. genotypes and pathogenicity, which are relevant to the host and provide useful insights for future vaccine development.
Dermatologic facial applications of Morpheus8 fractional radiofrequency microneedling
Authors: Hendricks Aleksi J. , Farhang Sheila Z. 
Published: August 2022

AbstractDermatologic patients are expressing increasing interest in minimally invasive procedures to address a wide range of common concerns from skin laxity to acne and scarring. Fractional radiofrequency (RF) microneedling offers an effective method for addressing a variety of dermatologic conditions with reduced downtime compared with historically more invasive procedural approaches. This article aims to describe the technology utilized in fractional RF microneedling (Morpheus8, InMode Aesthetics) and its studied applications in dermatology for treatment of the face.
The impact of ageing simulation education on qualified acute care nurses' empathy towards older people: A mixed‐methods study
Authors: Bowden Alera , Wilson Valerie , Traynor Victoria , Chang Hui‐Chen (Rita) 
Published: August 2022

AbstractAims and ObjectivesTo evaluate the influence of an ageing simulation intervention on qualified acute care nurses' empathy towards older people.BackgroundLife expectancy is increasing globally with an increased incidence of older people accessing healthcare services. As such, it is essential for qualified nurses within hospital settings to connect with older people by establishing healthful relationships. Empathy is an essential component of healthful nurse‐patient relationships. Fortunately, empathy can be taught through education.DesignA convergent mixed‐methods design was utilised.MethodsNurses (N = 95) undertook an 8‐hour ageing simulation intervention. Data were collected from April 2019 to May 2020 across three time points: before the intervention (T0); immediately after (T1) and at three months follow‐up (T2). A mix of convenience (intervention) and purposeful (follow‐up) sampling techniques were utilised. Quantitative data were collected via the Interpersonal Reactivity Index survey (n = 86) and analysed using repeat measures ANOVA to compare mean scores across time points. Qualitative data were collected via debriefing discussions (N = 95) and focus groups (n = 38), and analysed using a systematic thematic analysis method. Data convergence occurred during the interpretation phase. Study reported with the TREND checklist.ResultsPrimarily, when quantitative and qualitative findings were merged they confirmed each others' empathy outcomes. Quantitative results showed a statistically significant increase in affective and cognitive empathy levels among nurses post‐intervention. Qualitative findings expanded on quantitative results and revealed an increase in nurses' affective, cognitive and behavioural empathy represented in themes ‘enhancing my empathy’, ‘impact of ageing’, ‘from self to others’ and ‘person‐centred moments’.ConclusionsThis study adds empirical evidence how a mixed‐methods design can be used to evaluate the influence of an ageing simulation intervention on nurses' empathy levels.Relevance to clinical practiceAgeing simulation interventions are a suitable experiential educational approach to improve acute care nurses' affective, cognitive and behavioural empathy towards older people.
Military Adolescent Pandemic Study 2021: MAPS21
Authors: Thomas Jennifer S. , Trimillos Amanda , Allsbrook‐Huisman Stacy 
Published: August 2022

ABSTRACTBACKGROUNDMilitary‐connected students move between 6 to 9 times throughout their K‐12 experience, creating unique challenges relating to integration, adaptation, identity development, and acceptance. However, when transitions occur during a global pandemic, isolation and disconnect with schools and the community create new challenges that impact health and well‐being. The MAPS21 study uncovers the lived experiences of military‐connected students who have experienced a transition during the pandemic and gives rise to the need for interdisciplinary care within the public‐school setting to promote mental health and academic support during and post‐pandemic transitions.METHODSMilitary adolescents and their parent(s) were interviewed and data were analyzed following an interpretive phenomenological process. A social‐ecological model served as the guide to understand the participants' experiences and to further inform future supportive measures.RESULTSSeven subthemes surface under Individual, Relationships, Community, and Society and Policy, the levels within the social‐ecological model. These subthemes included: anticipatory processes, control, growth and maturity, adapting views, isolation, school support, community support.ConclusionMilitary‐connected students may experience adverse outcomes relating to mental health and academic progression after experiencing a move during the pandemic. This study provides an avenue for teams of interdisciplinary professionals to collaborate within the public‐school setting to provide proactive support through engagement and policy development.
The Impact of Distance Education during the COVID‐19 Pandemic on Physical Activity and Well‐Being of Czech and Polish Adolescents
Authors: Frömel Karel , Groffik Dorota , Valach Petr , ŠafáŘ Michal , MitáŠ Josef 
Published: August 2022

ABSTRACTBACKGROUNDThe aim of this study was to identify the changes in the structure of weekly physical activity (PA) and well‐being among adolescent boys and girls between habitual education (HE) and distance education (DE) during the pandemic in secondary schools.METHODSThe research was carried out in 12 Czech and 18 Polish schools during 2019 to 2020 academic session for HE and 2020 to 2021 academic session for DE. The research involved 723 girls and 626 boys aged 15 to 18 years. The structure of the weekly PA was assessed using the International Physical Activity Questionnaire‐Long Form, while well‐being was assessed using the World Health Organization‐5 Well‐Being Index.RESULTSThere was a statistically significant decrease of PA among boys in school PA, transportation PA, recreation PA, vigorous PA, moderate PA, walking, and overall weekly PA during the DE resulting from the pandemic. In girls, during DE, a decrease was observed in school PA, vigorous PA, and overall PA. During DE, a high level of well‐being was reported by only 34.9% of girls (43.8% during HE) and 50% of boys (65.6% during HE).CONCLUSIONSThe observed low PA and high incidence of depressive symptoms in adolescents in distance education during pandemic constraints are a challenge for schools to change in supporting physical activity, eliminating similar negative impacts on school life in the future.
Ownership and media slant: Evidence from Swedish newspapers
Authors: Garz Marcel , Rickardsson Jonna 
Published: August 2022

AbstractThis study investigates the role of media owners for the political bias of newspapers in Sweden, using an original dataset on outlets, consumer preferences, and ownership between January 2014 and April 2019. We construct an index of slant based on similarities in the language between newspapers and speeches given by members of parliament. Our results indicate that newspapers held by the same owner tend to offer the same mix of slant, rather than aligning their bias with consumer preferences in their area of circulation. Owners are even less inclined to differentiate the slant across outlets before elections, when the political returns to persuasion are high. We find no evidence that owners impose a one‐size‐fits‐all slant because product differentiation is too costly. In addition, we find suggestive evidence of owner‐independent bias induced by the writers of opinion articles. The Swedish context illustrates that supply‐driven slant cannot be ruled out in market‐based media systems if the ties between media and politics are strong.
Plants acclimate to Photosystem I photoinhibition by readjusting the photosynthetic machinery
Authors: Lempiäinen Tapio , Rintamäki Eevi , Aro Eva‐Mari , Tikkanen Mikko 
Published: August 2022

AbstractPhotosynthetic light reactions require strict regulation under dynamic environmental conditions. Still, depending on environmental constraints, photoinhibition of Photosystem (PSII) or PSI occurs frequently. Repair of photodamaged PSI, in sharp contrast to that of PSII, is extremely slow and leads to a functional imbalance between the photosystems. Slow PSI recovery prompted us to take advantage of the PSI‐specific photoinhibition treatment and investigate whether the imbalance between functional PSII and PSI leads to acclimation of photosynthesis to PSI‐limited conditions, either by short‐term or long‐term acclimation mechanisms as tested immediately after the photoinhibition treatment or after 24 h recovery in growth conditions, respectively. Short‐term acclimation mechanisms were induced directly upon inhibition, including thylakoid protein phosphorylation that redirects excitation energy to PSI as well as changes in the feedback regulation of photosynthesis, which relaxed photosynthetic control and excitation energy quenching. Longer‐term acclimation comprised reprogramming of the stromal redox system and an increase in ATP synthase and Cytochrome b6f abundance. Acclimation to PSI‐limited conditions restored the CO2 assimilation capacity of plants without major PSI repair. Response to PSI inhibition demonstrates that plants efficiently acclimate to changes occurring in the photosynthetic apparatus, which is likely a crucial component in plant acclimation to adverse environmental conditions.
Evaluation of the immunoregulatory effect of Dicrocoelium dendriticum eggs on inflammatory and anti‐inflammatory cytokines in EAE model
Authors: Jafari Rad Mozhdeh , Navi Zahra , Heidari Amir Reza , Lavi Arab Fahimeh , Tabasi Nafiseh , Rastin Maryam , Khadem Rezaiyan Majid , Moghaddas Elham , Mahmoudi Mahmoud 
Published: August 2022

AbstractExperimental autoimmune encephalomyelitis (EAE) is an appropriate model for the study of the immunologic and pathologic mechanisms in multiple sclerosis (MS). According to the hygiene hypothesis, helminths can improve immunoregulation and have therapeutic effects on immune‐mediated diseases. In this study, we used Dicrocoelium dendriticum (Dicrocoeliidae, Platyhelminthes) eggs for the evaluation of their prophylactic and treatment effects on EAE disease. D. dendriticum eggs were extracted. Female C57BL/6 mice were immunized with the specific antigen MOG35−55, and then the egg extracts were utilized for prophylaxis and/or treatment. Clinical symptoms and other relevant parameters were assessed daily. The mRNA expression of transforming growth factor‐β (TGF‐β), interleukin‐10 (IL‐10), IL‐6, IL‐23 and IL‐17 were assessed with a real‐time polymerase chain reaction technique. Furthermore, secretion of TGF‐β and IL‐17 cytokines were determined by enzyme‐linked immunosorbent assay. Data indicated that clinical symptoms in prophylaxis and treatment groups were decreased significantly in comparison with the untreated control group (p < .001). Our results showed a significant decrease in IL‐17, as well as an increase in TGF‐β cytokine in the treatment group compared to the EAE control group (p < .01). Furthermore, in the prophylaxis and treatment groups, the mRNA expression of disease‐associated cytokines decreased and the mRNA expression of the anti‐inflammatory cytokines increased. In this study, the D. dendriticum egg ameliorates the clinical symptoms of the EAE model through the modulation of related cytokines of Th17 and Treg cells. Therefore, using this parasite egg could be a new treatment for MS.
Co‐expression networks of deacclimation‐impaired transcription factor mutants identified complex regulation of the cold stress release response
Authors: Vyse Kora , Fischer Axel , Zajancauskaite Greta , Erban Alexander , Kopka Joachim , Balazadeh Salma , Zuther Ellen 
Published: August 2022

AbstractPlants adapt to cold, non‐freezing temperatures through cold acclimation and lose acquired freezing tolerance in warmer temperatures in a process called deacclimation. This work identified a hypoxia response during early deacclimation evidenced by increased ADH activity and upregulated gene expression of hypoxia markers. Mutants defective in hypoxia response and transcription factors transiently induced during deacclimation, hra1, lbd41, mbf1c, and jub1, revealed slower deacclimation rates. Comparative transcriptome (RNA‐Seq) and co‐expression analysis of Col‐0, mbf1c, and jub1 pointed to an involvement of genes encoding thermoswitches, cell wall remodelers, ABC‐transporters, and H+‐ATPases in regulating the deacclimation rate. In mbf1c and jub1, unique increases of stress‐responsive genes and genes involved in jasmonic acid regulation were detected and linked to freezing tolerance retention during deacclimation. Data of this study are in agreement with previous observations of hypoxic niches within the plants and connected effects on growth and development during deacclimation. Regulation of deacclimation was shown to be a complex process, finely tuned to achieve an optimal rate and onset of deacclimation in plants. Candidate genes for future targeted analyses of this regulation are also suggested.
Why is there a lack of evidence regarding errors and complications in periodontal and implant therapy?
Authors: Chambrone Leandro , Zucchelli Giovanni 
Published: August 2022

AbstractThe occurrence of errors, complications, and adverse effects may occur as a consequence of single or multiple events related to the clinician and/or patient. Apparently, the amount of dental literature on these undesirable outcomes has not been as prolific as that obtained for conventional primary periodontal outcome measures. This review explores the potential reasons for the lack of studies reporting on errors and complications in periodontal and implant therapy, as well as other noteworthy methodological aspects, to enlighten their impact on the selection of the best (or most appropriate) “gold standard” periodontal/implant‐related treatment options, and on the overall decision‐making process. The following points were addressed: (a) the importance of reporting errors and complications in clinical research; (b) the adequate reporting of errors and complications in periodontology and dental implantology; and (c) efficacy trials vs effectiveness studies and their impact on the assessment and report of periodontal and implant treatment‐related risks and complications.
Anti‐Müllerian hormone as an endocrine biomarker of reproductive longevity and assessment of single nucleotide polymorphisms in AMH gene of Bos indicus breeds of cattle
Authors: Ramesha Kerekoppa P. , Jeyakumar Sakthivel , Mall Shweta , Vedamurthy Gowdar Veerappa , Kumaresan Arumugam , Devadasn Milton Joel , Kataktalware Mukund A. , Arul Dhayalan , Das Dayal Nitai , Manimaran Ayyasamy 
Published: August 2022

AbstractAnti‐Müllerian hormone (AMH) is a member of the TGF‐β superfamily produced by follicular granulosa cells in women and cattle and is considered an endocrine biomarker of ovarian follicular reserve. The study examined how age and parity influence serum AMH concentration and investigated the presence of single nucleotide polymorphisms in AMH gene in Bos indicus breeds viz Malnad Gidda Amritmahal and Hallikar. All five exons of AMH gene amplified by polymerase chain reaction were subjected to sanger sequencing and identified important SNP and its effects. We observed a highly significant relationship between parity and AMH concentration in Amritmahal cattle, whereas Malnad Gidda and Hallikar breeds did not show a significant difference. We identified one SNP located in exon 5 (rs21402788) with base change A>G, a non‐synonymous mutation resulting in a change in amino acid Q>R and the protein product. It is concluded that AMH level could be considered as an indicator of the ovarian reserve and productive herd life (longevity) irrespective of age/parity, especially in B. indicus breeds of cattle.
Extracranial schwannoma of the maxillary nerve misdiagnosed as trigeminal neuralgia in an elderly patient with schizophrenia – A diagnostic dilemma
Authors: Poorna T Anish , JR Lokesh , EK Joshna , John Bobby , S Neethu , S Shankar 
Published: August 2022

AbstractThe present paper discusses the diagnostic challenges we faced in a 60‐year‐old woman with a history of schizophrenia, presenting with left unilateral facial pain for the past three months. Based on the elaborate clinical examination and diagnostic nerve blocks, the patient was diagnosed with trigeminal neuralgia (TN) and non‐surgical therapy commenced. Further investigations with magnetic resonance imaging (MRI) and ultrasound‐guided fine needle aspiration cytology (FNAC) revealed the presence of an extracranial schwannoma involving a branch of the maxillary nerve. The patient was symptomatically relieved after surgical excision of the benign tumor under general anesthesia. Hence, we emphasize the need for special care and attention in psychiatric patients presenting with orofacial pain.
Digicel! Topap long ples ia! An international telecommunications company making itself at home in the urban landscapes of Vanuatu, Samoa and Tonga
Authors: Willans Fiona , Gure Jim , Vaifale Tereise , Veikune ' Elenoa 
Published: August 2022

AbstractMobile phone usage has increased at an unprecedented rate across the Pacific over the past 10–15 years, radically transforming the way communication takes place. The catalyst for this transformation is generally attributed to the breakdown of monopolies previously held by national telecom corporations over their own domestic markets, and the entrance of one particular new provider, Digicel. This paper examines the strategies through which Digicel has managed to insert itself into the visual landscape of the urban spaces of Vanuatu, Samoa and Tonga. Through multimodal analysis of digital photographs from the landscapes of these countries' capital cities, the paper shows how the global company makes use of a range of techniques to establish its own place and identity as a local network. These techniques include the demonstration of largesse and dominance over competitors, slogans that stake a claim to belonging, and the use of local language terms and images that juxtapose the local with the global. Through these techniques, Digicel manages to position itself simultaneously as the provider both of fast and reliable global communication technologies and of a truly local, national service, while also radically transforming the physical spaces of our cities as it has made itself at home here.