bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanobiology of the relocation of proteins in advecting cells: modeling, experiments, and simulations.
Authors: Serpelloni Mattia , Arricca Matteo , Ravelli Cosettta , Grillo Elisabetta , Mitola Stefania , Salvadori Alberto 
Published: September 2022

Abstract
Cell motility - a cellular behavior of paramount relevance in embryonic development, immunological response, metastasis, or angiogenesis - demands a mechanical deformation
of the cell membrane and influences the surface motion of molecules
and their biochemical interactions.
In this note, we develop a fully coupled multi-physics model able to capture and predict the
protein flow
on endothelial advecting plasma membranes.
The model has been validated against co-designed in vitro
experiments. The complete picture of the receptor dynamics
has been understood,
limiting factors have been identified together with the laws that regulate receptor
polarization.
This computational approach might be insightful in the prediction of endothelial cell behavior in different tumoral environments,
circumventing the time-consuming and expensive empirical characterization of each tumor.
3rd Trimester Umbilical Arterial Pulsatility Index Is Associated with Neurodevelopmental Outcomes at 2-years in Major Congenital Heart Disease.
Authors: MBBS Amol A MORAY , ROBERTSON Charlene M.T. , MN Gwen Y. BOND , ABEYSEKERA Jayani B. , MOHAMMADIAN Parsa , DINU Irina A. , ATALLAH Joseph , SWITZER Heather N. , HORNBERGER Lisa K. , Hornberger Lisa 
Published: September 2022

Abstract
Background
Major congenital heart disease (CHD) is associated with impaired neurodevelopment (ND), partly from prenatal insults. In this study we explore associations between 2nd and 3rd trimester umbilical (UA) and middle cerebral artery (MCA) pulsatility index (PI = systolic-diastolic velocities/mean velocity) in fetuses with major CHD and 2-year ND and growth outcomes.
Methods
Eligible patients included those with a prenatal diagnosis of CHD from 2007–2017 without a genetic syndrome who underwent previously defined cardiac surgeries and 2-year biometric and ND assessments in our program. UA and MCA-PI Z-scores at fetal echocardiography were examined for relationships with 2-year Bayley Scales of Infant and Toddler Development and biometric Z-scores.
Results
Data from 147 children was analyzed. Second and 3rd trimester fetal echocardiograms were performed at 22.4 ± 3.7 and 34.7 ± 2.9 weeks (mean ± SD), respectively. Multivariable regression analysis showed an inverse relationship between 3rd trimester UA-PI for all CHD and cognitive − 1.98(-3.37,-0.59), motor − 2.57(-4.15,-0.99), and language − 1.67(-3.3,-0.03) (effect size and 95th confidence interval) ND domains (p < 0.05), with the strongest relationships in the single ventricle and hypoplastic left heart syndrome subgroups. No association was found for 2nd trimester UA-PI or any trimester MCA-PI and ND or between UA or MCA-PI and 2-year growth parameters.
Conclusions
Increased 3rd trimester UA-PI, reflecting an altered late gestation fetoplacental circulation, relates to worse 2-year ND in all domains.
Preoperative Factors Affecting the Two-Year Postoperative Outcome in Single-Level Lumbar Grade I Degenerative Spondylolisthesis: Prospective, Multicenter, Patient-Preference Cohort Study using Patient-Reported Assessment
Authors: Kanchiku Tsukasa , Taguchi Toshihiko , Sekiguchi Miho , Toda Naofumi , Hosono Noboru , Matsumoto Morio , Tanaka Nobuhiro , Akeda Koji , Hashizume Hiroshi , Kanayama Masahiro , Orita Sumihisa , Takeuchi Daisaku , Kawakami Mamoru , Fukui Mitsuru , Kanamori Masahiko , Wada Eiji , Kato So , Hongo Michio , Ando Kei , Iizuka Yoichi , Ikegami Shota , Kawamura Naohiro , Takami Masanari , Yamato Yu , Takahashi Shinji , Watanabe Kei , Takahashi Jun , Konno Shinichi , Chikuda Hirotaka 
Published: September 2022

Abstract
Background
Selection of operative method for lumbar spinal stenosis with Meyerding grade I degenerative spondylolisthesis remains controversial and the preoperative factors affecting the patient-reported postoperative assessment are unknown. The objective of this study was to clarify the preoperative factors affecting the two-year postoperative outcome in Meyerding grade I degenerative spondylolisthesis by using a patient-reported assessment.
Methods
Seventy-two consecutive patients who had decompression alone (D group; 28) or with fusion (DF group; 44) were enrolled. The parameters investigated were the Japanese Orthopaedic Association back pain evaluation questionnaire, visual analog scales, and radiological evaluation of L4 slippage (L4S), lumbar lordosis (LL), and lumbar axis sacral distance (LASD) as an index of sagittal alignment. The prospectively collected data of postoperative 2 years were examined by statistical analysis.
Results
Finally, sixty-two cases (D group; 25, DF group; 37) were evaluated. There was no significant difference in JOABPEQ outcome between the two surgical groups. On the other hand, in multiple logistic regression analysis, gender, preoperative L4S, LASD, and LL were extracted as significant preoperative factors affecting the two-year postoperative outcome. Women had a lower rate of improvement in lumbar spine dysfunction due to low back pain (risk ratio = 0.17, p = 0.034) and psychological disability (risk ratio = 0.222, p = 0.045) compared to men. Patients with preoperative L4S greater than 5–6 mm have a lower rate of improvement in low back pain (risk ratio = 0.159, p = 0.049) and lumbar spine dysfunction due to low back pain (risk ratio = 0.188, p = 0.043). Patients with a preoperative LASD greater than 30 mm have a higher rate of improvement in postoperative low back pain (risk ratio = 20.905, p = 0.008) and lumbar dysfunction due to low back pain (risk ratio = 11.48, p = 0.021). Preoperative LL of less than 35 degrees was associated with a lower rate of improvement in gait disturbance due to low back pain (risk ratio of high lordosis to low lordosis = 11.638, p = 0.017).
Conclusions
In this study, the selection of operative method was not a significant factor affecting the two-year postoperative outcome and gender, preoperative L4S, LASD, and LL were extracted as significant preoperative factors.
Route of dexamethasone administration influences parasite burden in Strongyloides hyperinfection model
Authors: Corrêa Luisa Queiroz , Couto Bruna Patrícia do , de Carvalho Edson Fernando Goulart , de Sousa José Eduardo Neto , Ribeiro Vanessa da Silva , Gonzaga Henrique Tomaz , Costa-Cruz Julia Maria 
Published: September 2022

Abstract

Rodents infected withStrongyloides venezuelensisare experimental models applied to strongyloidiasis research. This study evaluated oral and subcutaneous dexamethasone (DEX) treatments to establish immunosuppression in an experimental model ofStrongyloideshyperinfection.Rattus norvegicusWistar were divided: G I (−): untreated and uninfected animals, G II (+): untreated and infected, G III (o −) orally treated and uninfected, G IV (o +) orally treated and infected, G V (sc −) subcutaneously treated and uninfected, G VI (sc +) subcutaneously treated and infected. For oral administration, DEX was diluted in sterile water (5 µg / ml) and made available to the animals on intervals in experimental days − 5 to 0, 8 to 13 and 21 to 26. For subcutaneous administration, animals received daily injections of DEX disodium phosphate (2mg / kg). Infection was established by the subcutaneous inoculation of 3000S. venezuelensisfilarioid larvae. Groups were evaluated by egg per gram of feces and parasite females counts and IgG, IgG1 and IgG2a detection. GIV (o+) had egg peaks count on days 13 and 26 and maintained egg elimination until the last experimental day. Parasitic females recovery at day 30 was significantly higher in G IV (o+) when compared to G VI (sc +). Levels of IgG, IgG1 and IgG2a of all groups, except the positive control GII (+), were below the detection threshold. Pharmacological immunosuppression induced by oral administration of DEX produced high parasitic burden, and is a noninvasive method, useful to study immunosuppression in strongyloidiasis hyperinfection model in rats.
Antimicrobial Activity of Bacillus velezensis K-9 Against Potato Scab and Its Genome-wide Analysis
Authors: Shuang Ma , Wang Yanjie , Teng Wang , Ru Jiarong , Jiang Lili , Cong Wang 
Published: September 2022

Abstract
We isolated a bacterial strain K-9, identified as a species of the genus Bacillus, from a potato tuber sample. The inhibition rate of the strain K-9 against potato scab (Streptomyces scabies) was 44.90%. The whole genome sequence of Bacillus velezensis K-9 was obtained, and genomic analyses were performed. Complete genome sequence of strain K-9 was obtained through PacBio RSII and Illumina platforms. The size of genome is 3891530 bp, with estimated 3915 coding DNA sequences, GC content of 46.45%, 79 tRNA, and 10 rRNA. In addition, functional annotation of the strain K-9 genes was performed by GO, COG, KEGG, and CAZy analyses. There were 12 gene clusters for secondary metabolite synthesis in the genome of Bacillus velezensis K-9. Except for the unknown metabolites of the T3PKS (third polyketone), terpene and lanthipeptide class-II, the other eight gene clusters are associated with the synthesis of secondary metabolites that are common in Bacillus and have antibacterial activity. Based on the functional analysis of the genome, the lipopeptide extract and crude protein extract of the strain were verified as bacteriostatic. The metabolites of its antibacterial activity were analyzed and identified by metabolomics technology. The antibacterial protein-related substances such as polyketide synthetase, plastin synthetase, bacitin-biosynthetic protein and flagellin were isolated and identified from the crude protein extract. In the future, information derived from the thorough genomic analyses of this strain may be helpful in directed genetic modification of bacterial strains for production of antibacterial substances to control potato scab.
Assigning Resistivity Values to Rock Quality Designation indices Using Integrated Unmanned Aerial Vehicle and 2D Electrical Resistivity Tomography in Granitic Rock
Authors: Junaid Muhammad , Abdullah Rini Asnida , Saa’ri Radzuan , Islam Amber 
Published: September 2022

Abstract

Rock quality designation (RQD) is a standard technique in mining and geotechnical investigation for quantifying the quality and degree of jointing of the rock mass. However, the need for expeditious and inexpensive geotechnical site characterization is the main motivation for the modification of RQD. This research work integrates 2D Electrical Resistivity Tomography (2D ERT), Unmanned Aerial Vehicle (UAV) and borehole to assign resistivity values to various RQD indexes. The UAV survey was performed to calculate RQD on the rock surface using volumetric joint counts (Jv), whereas the 2D ERT survey provides the corresponding resistivity values. A limited number of core samples were also gathered to validate the o the 2D ERT investigation. Combine 2D ERT and UAV provides resistivity values for various RQD indices such as very poor rocks (<350 Ωm), poor rock (350-1150 Ωm), fair rock (1150 – 1850 Ωm), good rock (1850 -2500 Ωm) and > 2500 Ωm excellent rock. Based on the established correlation of RQD and resistivity, the subsurface rock mass quality at Site 1 was predominantly good and excellent rock, while at Site 2, poor rock was significantly reported. This research study concluded that rock mass characterization using RQD is more rapid and inexpensive than the direct or indirect calculation of RQD.
Functional improvements in β-conglycinin by preparing edible bioconjugates with ε-polylysine and dextran
Authors: Yoshida Tadashi , Hamaji Ikumi , Hashimoto Takeshi , Matsumoto Takuya , Hattori Makoto 
Published: September 2022

Abstract
β-Conglycinin was conjugated with ε-polylysine (PL) by means of microbial transglutaminase (MTGase) to improve its function. The β-conglycinin-PL conjugate was purified by dialysis. Composition of the β-conglycinin-PL was β-conglycinin:PL = 1:18 (molar ratio) which was confirmed by amino acid analysis. The β-conglycinin-PL was further conjugated with dextran (Dex) by the Maillard reaction. The β-conglycinin-PL-Dex conjugate was purified by dialysis. Conjugation was confirmed by SDS-PAGE and PAS staining. Composition of the β-conglycinin-PL-Dex was β-conglycinin-PL:Dex = 1:41 (molar ratio) which was confirmed by UV spectra measurement and phenol sulfuric acid method. Solubility of β-conglycinin in the acidic range was much improved by conjugation with PL and further improved by further conjugation with Dex. Emulsifying property of β-conglycinin in acidic pH range was much improved by conjugation with PL and Dex. Immunogenicity of β-conglycinin was decreased by conjugation with PL and Dex.
Effectiveness of the virtual psychosocial empowerment program on the quality of life of family caregivers of chronic mental disorders: a quasi-experimental study
Authors: lohrasebi fatemeh , Maghsoudi Jahangir , Alavi Mousa , Akbari Mohammad 
Published: September 2022

Abstract
Introduction: Caring for a patient with a chronic mental disorder is a difficult and exhausting task that makes family caregivers face all kinds of psychosocial problems and ultimately reduces the quality of life of these people. This study aimed to investigate the effect of the virtual psychosocial empowerment program on the quality of life of family caregivers with chronic mental disorders.Methods: This is a quasi-experimental study with a control group and a pre-test-post-test design that was performed on 70 family caregivers of patients with chronic mental disorders in Farabi and Noor educational- medical centers in Isfahan, Iran. The participants in this study were selected by convenience sampling method. Data were collected using a demographic information form and the WHO-QOL-BREF questionnaire, before, immediately, and one month after the intervention. The experimental group received the psychosocial empowerment program in 8 sessions and 4 weeks. At the end of the intervention, and one month later the WHO-QOL-BREF questionnaire was completed by the experimental and control groups and the data were analyzed using independent t-test and RM-ANOVA.Results: In this study, 35 participants were in the intervention group and 35 participants were in the control group. Three members of the intervention group were excluded from the study and finally data analysis was performed on 67 of the samples. The average quality of life score and its dimensions in the intervention group were improved immediately after the intervention and one month after that. The results of the RMANOVA test showed that the mean changes in the intervention group were significant(P=0.000), but these changes were not significant in the control group (P=0.793). Conclusions: Using the empowerment program for family caregivers of chronically mentally ill patients in virtual form can be effective on their quality of life and can be used as one of the supported methods for these families.
Improvement effect of enzymic polypeptide from Paeonia ostii cake on protein-energy malnutrition mouse model
Authors: Wei Liang , Yang Huiying , Yu Xinxin , Pu Duowen , Lu Qi , Zhu Minghua , Zu Yuangang 
Published: September 2022

Abstract
The improvement effects of enzymatic polypeptide of Paeonia ostii cake (APHP) on model mice of protein-energy malnutrition were evaluated by body weight changes, organ indices, levels of SOD, CAT，MDA, total protein and albumin in serum, staining images of major organs and macrophages phagocytosis. The results indicated the mice body weight recovery was dose-dependent with APHP and the body weight change of model mice fed with high-dose APHP (5.0 g/kg) and normal diet showed a same recovery effect. The model mice had higher thymus and spleen indexes than other groups. And the values of SOD, CAT, MDA, total protein and albumin in serum returned to normal level after 30, 60, 45, 30 and 45 days respectively, which was better than those of normal diet. Our research will provide basic data support for new potential applications of APHP and increase the added value of Paeonia ostii processing enterprises.
Genome-Wide Identification, Evolution Analysis of PI-PLC Family and Their Expression Patterns in Response to Different Hormones and Growth in Banana (Musa L.)
Authors: Wu Qiuzhen , Fan Zhengyang , Qi Feng , Li Dan , Zhang Zihao , Chen Yukun , Huang Yuji , Lin Yuling , Lai Zhongxiong 
Published: September 2022

Abstract
PI-PLC is not only involved in the regulation of plant responses to a variety of biotic and abiotic stresses, but also plays an important role in the regulation of plant growth. banana is one of the four most famous fruits in the world, as well as one of the most important tropical fruits in the world. However, in recent years, blight and cold damage have become two major deadly hazards for banana industry, making it particularly important to improve the resistance of bananas to stress. In this study. PI-PLCs gene family of banana encodes 567–698 amino acidsand. It is mainly located in chloroplasts, mitochondria and cytoplasm. Systematic evolution and homology analysis, PI-PLCs gene family of banana is highly clustered, the structure and function are relatively consistent. There were hormones and stress response elements such as methyl jasmonate, auxin and gibberellin, in the promoter region of MaPLCs family members. The proteins coded by all MaPLCs could interact and participate in a series of life-sustaining activities. The study showed that members of the MaPLCs gene family of bananas could be induced to express by auxin, abscisic acid and salicylic acid. And the leaves appeared curl, wilting symptoms, and water-stained spots.
Penetrating Keratoplasty in Congenital Glaucoma
Authors: Tamçelik Nevbahar , Oto Bilge Batu , Bozkurt Ercüment , Arici Ceyhun , Kılıçarslan Oğuzhan , Gönen Busenur , Çelik Hacı Uğur 
Published: September 2022

Abstract
Purpose: To investigate the graft survival rate and factors affecting graft survival in patients with congenital glaucoma who underwent penetrating keratoplasty (PKP).Materials and Methods: Patients with congenital glaucoma who underwent PKP were retrospectively evaluated. The associations between age, corneal diameter, presence of ocular comorbidities, concurrent ocular surgeries with corneal graft, and visual outcomes were assessed.Results: Among the 30 eyes enrolled in the study, 6 (20%) had aniridia, 6 (20%) had Axenfeld–Rieger syndrome, and 18 (60%) were diagnosed with primary congenital glaucoma. Graft survival rates were 66.6% and 63.33% at 12 and 24 months, respectively. At the end of the follow-up, the overall graft survival rate was 60%. Statistical significance was observed between patient age at the time of surgery and graft failure (p=0.02). Graft failure was associated with a younger patient age. Functional vision was achieved in 53.3% of patients.Conclusions: Management of congenital glaucoma and its corneal complications is a delicate issue that requires great effort. PKP in congenital glaucoma was moderately successful in the present study. To provide functional vision, PKP could be the treatment of choice.
D-limonene binds the target gene odorant-binding protein 3 to control Bemisia tabaci
Authors: Wei Yan , Gao Liming , Zhang Zhanhong , Xu Lingchao , Zhang Zhuo , Zhang Deyong , Chen Jianbin , Li Kailong , Yan Shuo , Du Jiao , Zhou Xuguo , Shi Xiaobin , Liu Yong 
Published: September 2022

Abstract
Bemisia tabaci (Gennadius) is an important global invasive pest, which damages plants by feeding on phloem sap and causes great losses to agricultural production. At present, the management of B. tabaci still relies on insecticides. Based on the emergence of the insecticide resistance and the pursuit of environmental friendliness, more green control methods have been generated. Insects rely on odorant-olfactory proteins to identify specific volatiles and locate hosts, and these volatiles can be exploited in agricultural pest control. This study evaluated the control efficacy of d-limonene, a common volatile in three avoidant plants. Our results showed that d-limonene had a repelling effect on B. tabaci, and the LC50 value of d-limonene was 81.623 ppm. Moreover, the LC50 of bifenthrin and flupyradifurone were reduced by 25.2% and 38.7% when mixed with d-limonene. The underlying molecular mechanism of d-limonene recognition by B. tabaci was investigated. Treating B. tabaci with dsBtabOBP3 increased the control effect of d-limonene, and the LC50 of d-limonene, mixture (L+B) and mixture (L+F) were decreased by 32.6%, 38.4% and 33.1%. These results indicated that specific volatiles could directly bind to target genes and therefore enhance the control effect of B. tabaci.
Analysis of the curative effect of cervical spondylotic radiculopathy with osseous foraminal stenosis using ultrasonic osteotome in anterior cervical surgery
Authors: Liu Junlin , Kong Qingquan , Feng Pin , Zhang Bin , Ma Junsong , Hu Yuan 
Published: September 2022

Abstract
Purpose
To explore the clinical efficacy and operation points of cervical radiculopathy with osseous foraminal stenosis treated with ultrasonic osteotome in anterior cervical surgery.
Methods
From January 2018 to June 2021, a retrospective analysis of 23 patients with cervical radiculopathy with bony foraminal stenosis during this period was retrospectively analyzed. Anterior Cervical Discectomy and Fusion (ACDF) was used for all cases in this group. Intraoperative use of ultrasonic osteotome to decompress the nerve in the intervertebral foramina. The operation time, intraoperative blood loss and complication rate were recorded in this group of patients. Interbody fusion was evaluated using Brantigan criteria. The IC-PACS imaging system was used to measure the intervertebral foramen area (IFA) before and after surgery to evaluate the range of decompression. The VAS (Visual Analogue Scale, VAS) score and NDI (Neck Disability Index, NDI) score before and after surgery were recorded to evaluate the clinical efficacy.
Results
All enrolled patients were followed up regularly for 1 year or more. The mean operative time was 61.5 ± 8.0 minutes. The average intraoperative blood loss was 88.3 ± 12.8ml, and the average hospital stay was 8.1 ± 1.7d. 21 cases of successful fusion were followed up 1 year after operation, and the fusion rate was 91.3%. IFA expanded from 25.1 ± 4.0mm2 before operation to 57.9 ± 3.4mm2 at 1 year after operation, and the difference was statistically significant (P < 0.001). The VAS score and NDI score of patients 3 days after surgery, 3 months after surgery, and 1 year after surgery were significantly lower than those before surgery (P < 0.001). There was 1 case of dysphagia and 1 case of Cage subsidence after operation, and the complication rate was 8.6%.
Conclusion
Anterior cervical surgery using ultrasonic osteotome in the treatment of cervical radiculopathy with bony foraminal stenosis has reliable clinical efficacy and high safety, and is worthy of clinical promotion.