bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep Learning for Clustering Single-cell RNA-seq Data
Authors: Zhu Yuan , Bai Litai , Ning Zilin , Fu Wenfei , Liu Jie , Jiang Linfeng , Fei Shihuang , Gong Shiyun , Lu Lulu , Deng Minghua , Yi Ming 
Published: December 2022

Abstract:
The development of single-cell RNA sequencing (scRNA-seq) technology provides an
excellent opportunity to explore cell heterogeneity and diversity. With the growing application of
scRNA-seq data, many computational clustering methods have been developed to further uncover
cell subgroups, and cell dynamics at the group level. Due to the characteristics of high dimension,
high sparsity and high noise of the scRNA-seq data, it is challenging to use traditional clustering
methods. Fortunately, deep learning technologies characterize the properties of scRNA-seq data well
and provide a new perspective for data analysis. This work reviews the most popular computational
clustering methods and tools based on deep learning technologies, involving comparison, data collection,
code acquisition, results evaluation, and so on. In general, such a presentation points out some
progress and limitations of the existing methods and discusses the challenges and directions for further
research, which may give new insight to address a broader range of new challenges in dealing
with single-cell sequencing data and downstream analysis.
Colonic Degradation as Reverse Process to Flavone Biosynthesis in Plants:
Similarities and Differences
Authors: Sak Katrin 
Published: December 2022

Background:
For many years, it was thought that the main function of the colon is the
reabsorption of water and salt and the elimination of unused food materials. Only very recently, a
crucial role of the human intestinal microbiota in the metabolism of different food constituents,
including plant foods-derived flavonoids, was discovered. Currently, the knowledge about colonic
degradation of ingested flavonoids, involved bacteria and produced catabolites is rapidly increasing.
In general, flavonoids unabsorbed in the small intestine reach the colon, where they are exposed to
the gut microbiota.

Conclusion:
In this perspective article, colonic degradation of flavonoids is considered a reverse
process to their biosynthesis in plants, with a special focus on the subclass of flavones. According
to this approach: what is composed in plants, will be decomposed in the human colon. Several
inverse similarities are highlighted, including hydrolysis of flavonoid glycosides as the first step in
the gut degradation contrasted with the attachment of sugar moiety as the last reaction of flavonoid
biosynthesis in plants, colonic reduction contrasted with plant introduction of C2-C3 double bond
in the central heterocyclic ring, or microbial ring fission contrasted with plant ring closure of the
heterocyclic ring of flavones. Despite these inverse similarities, precursors of flavonoid pathway in
plants are different from the spectrum of gut microbial catabolites in humans. In the human colon,
a wide variety of phenolic acids are produced from the ingested flavonoids, due to the diverse enzymatic
capacity of intestinal microbiota. The bioactivities and potential health impacts of these
catabolites are still largely unknown.
Using Twitter Data to Estimate the Prevalence of Symptoms of Mental Disorders in the United States During the COVID-19 Pandemic: Ecological Cohort Study
Authors: Cai Ruilie , Zhang Jiajia , Li Zhenlong , Zeng Chengbo , Qiao Shan , Li Xiaoming 
Published: December 2022

Background
Existing research and national surveillance data suggest an increase of the prevalence of mental disorders during the COVID-19 pandemic. Social media platforms, such as Twitter, could be a source of data for estimation owing to its real-time nature, high availability, and large geographical coverage. However, there is a dearth of studies validating the accuracy of the prevalence of mental disorders on Twitter compared to that reported by the Centers for Disease Control and Prevention (CDC).

Objective
This study aims to verify the feasibility of Twitter-based prevalence of mental disorders symptoms being an instrument for prevalence estimation, where feasibility is gauged via correlations between Twitter-based prevalence of mental disorder symptoms (ie, anxiety and depressive symptoms) and that based on national surveillance data. In addition, this study aims to identify how the correlations changed over time (ie, the temporal trend).

Methods
State-level prevalence of anxiety and depressive symptoms was retrieved from the national Household Pulse Survey (HPS) of the CDC from April 2020 to July 2021. Tweets were retrieved from the Twitter streaming application programming interface during the same period and were used to estimate the prevalence of symptoms of mental disorders for each state using keyword analysis. Stratified linear mixed models were used to evaluate the correlations between the Twitter-based prevalence of symptoms of mental disorders and those reported by the CDC. The magnitude and significance of model parameters were considered to evaluate the correlations. Temporal trends of correlations were tested after adding the time variable to the model. Geospatial differences were compared on the basis of random effects.

Results
Pearson correlation coefficients between the overall prevalence reported by the CDC and that on Twitter for anxiety and depressive symptoms were 0.587 (P<.001) and 0.368 (P<.001), respectively. Stratified by 4 phases (ie, April 2020, August 2020, October 2020, and April 2021) defined by the HPS, linear mixed models showed that Twitter-based prevalence for anxiety symptoms had a positive and significant correlation with CDC-reported prevalence in phases 2 and 3, while a significant correlation for depressive symptoms was identified in phases 1 and 3.

Conclusions
Positive correlations were identified between Twitter-based and CDC-reported prevalence, and temporal trends of these correlations were found. Geospatial differences in the prevalence of symptoms of mental disorders were found between the northern and southern United States. Findings from this study could inform future investigation on leveraging social media platforms to estimate symptoms of mental disorders and the provision of immediate prevention measures to improve health outcomes.
Building Digital Literacy in Older Adults of Low Socioeconomic Status in Singapore (Project Wire Up): Nonrandomized Controlled Trial
Authors: Ngiam Nerice Heng Wen , Yee Wan Qi , Teo Nigel , Yow Ka Shing , Soundararajan Amrish , Lim Jie Xin , Lim Haikel A , Tey Angeline , Tang Kai Wen Aaron , Tham Celine Yi Xin , Tan Jamaica Pei Ying , Lu Si Yinn , Yoon Sungwon , Ng Kennedy Yao Yi , Low Lian Leng 
Published: December 2022

Background
In a rapidly digitalizing world, the inability of older adults to leverage digital technology has been associated with weaker social connections and poorer health outcomes. Despite the widespread digital adoption in Singapore, older adults, especially those of lower socioeconomic status (SES), still face difficulties in adopting information and communications technology and are typically digitally excluded.

Objective
We aimed to examine the impact of the volunteer-led, one-on-one, and home-based digital literacy program on digital literacy and health-related outcomes such as self-reported loneliness, social connectedness, quality of life, and well-being for older adults of low SES.

Methods
A nonrandomized controlled study was carried out in Singapore between July 2020 and November 2021 involving 138 digitally excluded community-dwelling older adults aged ≥55 years and of lower SES. Older adults awaiting participation in the program served as controls. Older adults under the intervention were equipped with a smartphone and cellular data, underwent fortnightly to monthly digital literacy training with volunteers to learn digital skills, and digitally connected to their existing social networks. Primary outcome was the improvement in self-reported digital literacy. Secondary outcomes included improvements in University of California, Los Angeles 3-item loneliness scale, Lubben Social Network Scale-6, EQ-5D-3L and EQ visual analogue scale scores, and Personal Wellbeing Score.

Results
There were significant improvements in digital literacy scores in the intervention group as compared to controls (mean difference 2.28, 95% CI 1.37-3.20; P<.001). Through multiple linear regression analyses, this difference in digital literacy scores remained independently associated with group membership after adjusting for differences in baseline scores, age, gender, education, living arrangement, housing type, and baseline social connectivity and loneliness status. There was no statistically significant difference in University of California, Los Angeles 3-item loneliness scale, Lubben Social Network Scale-6, Personal Wellbeing Score, or EQ-5D Utility and visual analogue scale score.

Conclusions
This study adds to the growing research on digital inclusion by showing that a volunteer-led, one-on-one, and home-based digital literacy program contributed to increase digital literacy in older adults of low SES. Future studies should look into developing more older adult–friendly digital spaces and technology design to encourage continued digital adoption in older adults and, eventually, impact health-related outcomes.
INHIBITORY EFFECT OF CURCUMIN INCORPORATED IN CHITOSAN NANOPARTICLE ON α-AMYLASE AND α-GLUCOSIDASE ACTIVITIES
Authors: WULANDARI WINDA T , TUSLINAH LILIS , APRILLIA ADE Y , ANGGRAENI RATNA , BUDIANI SHAFA A 
Published: December 2022

Objective: This research was conducted to determine inhibition activity of curcumin nanoparticles against α-amylase and α-glucosidase enzymes.
Methods: Curcumin nanoparticle was made by ionic gelation method using chitosan as cation, sodium tripolyphosphate as polyanion, and tween 80 as surfactant. Curcumin nanoparticles were tested for inhibitory activity of α-amylase and α-glucosidase enzymes using UV-Vis spectrophotometry at λ= 595 nm and 305 nm, respectively.
Results: Curcumin nanoparticles produced have 198.1 nm of particle size with PdI value of 0.349 and zeta potential value of-8,33 mV. The IC50 value of curcumin nanoparticles against α-amylase was 56.140 ppm, while acarbose was 63.32 ppm. While the IC50 value against α-glucosidase was 3.95 ppm and 4.11 ppm for curcumin nanoparticles and acarbose, respectively.
Conclusion: It can be concluded that curcumin nanoparticles have great potential as antihyperglycemic by inhibiting α-amylase and α-glucosidase enzymes.
IN SILICO STUDY OF 12 PHYTOSTEROL COMPOUNDS IN MORINGA (MORINGA OLEIFERA LAMK.) SEED OIL ON 5Α-REDUCTASE ENZYME INHIBITION ACTIVITY AS ANTI-ALOPECIA
Authors: KORASSA YULIUS BAKI , SAPTARINI NYI MEKAR , MUSTARICHIE RESMI , HENDRIANI RINI , TJITDA PUTRA JIWAMURWA PAMA 
Published: December 2022

Objective: This study aimed to determine the interaction of 12 phytosterol compounds in moringa seed oil to the 5α-reductase enzyme (PDB code: 7BW1) as anti-alopecia by in silico.
Methods: The research was conducted using a molecular docking approach using autodock Tools 1.5.6. Data analysis was carried out by looked at the binding affinity values ​​and inhibition constants (Ki) of 12 phytosterol compounds, as well as visualization of amino acid interactions using Biovia Discovery Studio 2021.
Results: 12 Phytosterol compounds had the potential to be a candidate for anti-alopecia medicines based on in silico test simulations using auto dock with high binding affinity values ​​in the range of-11.47 to-12.76 kcal/mol and stable inhibition constants in the range of 1.87. nM–4.30 nM involving hydrogen bonds with Arg179, Tyr178, Arg105, Arg114, Ser177, Tyr98, Glu57, and Tyr91 amino acids.
Conclusion: Ergostadienol compound in moringa seed oil was predicted to be a better anti-alopecia on the inhibition of 5α-reductase enzyme with binding energy value was-11.60 kcal/mol, inhibition constant was 3.17 nM and interaction of amino acid residues on the inhibition of 5α-reductase enzyme was similar with native finasteride ligands namely Glu57 and Tyr91.
FORMULATION AND CHARACTERIZATION OF FACIAL SERUM FROM ASTAXANTHIN-BETA CAROTENE NANOEMULSION AS AN ANTIOXIDANT
Authors: NURDIANTI LUSI , WULANDARI WINDA TRISNA , CAHYATI KENI IDA , SETIAWAN FAJAR 
Published: December 2022

Objective: The purpose of this study is to make facial serum from astaxanthin-beta carotene nanoemulsion as an antioxidant.
Methods: Nanoemulsions were prepared using Spontaneous Nanoemulsion (SNE) method with a ratio of 1:8:1 for (Sunflower oil): surfactant (Kolliphor® RH40): and co-surfactant (PEG 400). Physical nanoemulsion characterization includes globule size, polydispersity index, zeta potential, visual appearance, pH and entrapment efficiency test. The best results from nanoemulsion were then combined into serum preparations which were then tested for evaluation of the preparations, including organoleptic, homogeneity, viscosity, pH, adhesion and stability test (cycling test).
Results: The results showed that the nanoemulsion of astaxanthin and beta-carotene combination that had been developed had a globule size of<50 nm (with a normal globule size distribution curve), polydispersity index value was less than 0.5, zeta potential was greater than-20 mV and entrapment efficiency was ranging from 80-85%. The results of the preparation evaluation showed that serum astaxanthin-beta carotene nanoemulsion had good results in physical, chemical and stability test during storage. The best serum formula is formula 3 when viewed from the results of the evaluation of the dosage form with an IC50 value of 8.562 ppm with a very strong category as an antioxidant.
Conclusion: Facial serum from astaxanthin-beta carotene is the potential to be an antioxidant.
FORMULATION OF GARGLE FROM TRIGONA SP. PROPOLIS EXTRACT AND ITS ACTIVITY AGAINST STREPTOCOCCUS MUTANS
Authors: KURNIAWANSYAH INSAN SUNAN , CHAERUNISAA ANIS YOHANA , BUDIMAN ARIF 
Published: December 2022

Objective: In this research, the formulations of a gargle with Trigona sp. propolis extracts as an alternative of dental caries therapy and test its activity against Streptococcus mutans was carried out.
Methods: The experimental laboratory have been carried out with the following stages of work, material collection and processing, extraction of Trigona sp. propolis by maceration with 95% ethanol, phytochemical screening, determination of minimum inhibitory concentration, formulation of the gargle, activity, contact time and stability test of the formulas.
Results: Phytochemical screening showed that Trigona sp. propolis extracts containing flavonoids, polyphenols, quinones, monoterpenoids and sesquiterpenoids. Minimum inhibitory concentration was 0.25% w/v. The formulation of gargle from Trigona sp. propolis extract were a yellow solution with mint odor and taste of mint and sweet. The antibacterial activity of gargle preparation and formulas from the market as a comparison, showed that the formula with a concentration of 1% w/v gave a significant difference to the market preparation. The formulation of gargle from Trigona sp. propolis extract has good inhibition against bacteria S. mutans with a contact time of 60 seconds and is physically stable during the 35 d of storage time.
Conclusion: The formulation of gargle from Trigona sp. propolis extract is an excellent preparation to be developed in the future in the treatment of dental caries.
FORMULATION AND CHARACTERIZATION OF ZEAXANTHIN NANOEMULSION RADIANCE SERUM AS ANTIOXIDANT
Authors: SETIAWAN FAJAR , RUSDIANA TAOFIK , GOZALI DOLIH , NURDIANTI LUSI , IDACAHYATI KENI , WULANDARI WINDA TRISNA 
Published: December 2022

Objective: This study aimed to increase the effectiveness of using zeaxanthin by developed nanoemulsion containing zeaxanthin which was then formulated into radiance serum for topical use.
Methods: Nanoemulsions are made using spontaneous nano emulsification methods/techniques, which are relatively simple technologically because they rely on the right combination of selected surfactants and co-surfactants. The optimization of the formula was carried out starting from determining the oil: surfactant: co-surfactant phase ratio and concentration of zeaxanthin. Nanoemulsion characterization in the form of physical characterization includes organoleptic tests, globule size and polydispersity index, zeta potential, pH and entrapment efficiency tests. The best results from nanoemulsion were then combined into serum preparations which were then tested for evaluation of the preparations, including organoleptic, homogeneity, viscosity, pH, spreadability, and antioxidant test.
Results: The results showed that the developed zeaxanthin nanoemulsion had a globule size in the range of 20-24 nm (with a normal globule size distribution curve), polydispersity index value of less than 0.3, zeta potential greater than (-20) mV and entrapment efficiency ranging from 80-85%.
Conclusion: The results of the evaluation showed that the serum radiance of zeaxanthin nanoemulsion had good physical, chemical and stability properties during storage with an IC50 value of zeaxanthin less than 50 ppm.
NADES EXTRACT OF GEDONG MANGO LEAVES AND MULBERRY LEAVES IN SPRAY GEL AS A SUNSCREEN
Authors: RIZKIYAN YAYAN , SUHARYANI INE , FALYA YUNIARTI , AMELIA RENNY , NUH MUHAMMAD , SULASTRI LELA , INDAWATI IIN 
Published: December 2022

Objective: This study's purpose was to formulate a spray gel containing the combination extract of gedong mango leaves and mulberry leaves as a sunscreen preparation.
Methods: Each leaf was extracted by Natural Deep Eutectic Solvent-Maceration Assisted Extraction (NADES-MAE) method. The extract (gedong mango and mulberry leaves) formulated in Carbopol Spray Gel/GM-CarSG) in a ratio of GL and ML were 3:0.5 (GM-CarSG 1); 3:3 (GM-CarSG 2); and 3:6 (GM-CarSG 3). Furthermore, the spray gel was evaluated, and the SPF value was determined.
Results: The physical appearance, spreadability, pH, and viscosity of GM-CarSG 1, GM-CarSG 2, and GM-CarSG 3 meet the requirements. In addition, the three preparation have a sunscreen activity, with the SPF value of GM-CarSG 1, GM-CarSG 2, and GM-CarSG 3 being 2.08+0.03; 2.78+0.05; and 3.53+0.04, respectively.
Conclusion: The results showed that the combination of GL and ML extract is potentially used as a sunscreen in spray gel preparation. The best formulation is GM-CarSG 3, which has the highest SPF value among them.
IN SILICO STUDY OF BIOACTIVE COMPOUNDS FROM SUNGKAI (PERONEMA CANESCENS) AS IMMUNOMODULATOR
Authors: RAHARDHIAN MUHAMMAD RYAN RADIX , SUSILAWATI YASMIWAR , MUSFIROH IDA , FEBRIYANTI RADEN MAYA , MUCHTARIDI  , SUMIWI SRI ADI 
Published: December 2022

Objective: This study aims to predict a bioactive compound from Peronema canescens (PC) with mechanisms inhibitor interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) potential as an immunomodulatory using in silico approach.
Methods: Autodock 4 was used to accomplish computer-assisted drug design with molecular docking simulation to discover binding energy, inhibition constant, and interactions with an amino acid in bioactive compounds from PC against IL-6 and TNF-α receptors. Lipinski predicts the drug-likeness of a bioactive compound for the oral route of administration. ADMET profiling of bioactive compounds to predict pharmacokinetic properties with pkCSM ADMET.
Results: The results showed that the best binding energy, inhibition constant, and interactions with an amino acid of peronemin C1 against IL-6 and TNF-α receptors were (-7.19 kcal/mol; 5.39 nM; Arg 179, Arg 182, Gln 175), and (-8.86 kcal/mol; 320.42 nM; Tyr 119, Tyr 59, and Gly 121), respectively. All bioactive compounds from PC met Lipinski's rule of five requirements for oral administration. ADMET prediction results all bioactive compounds from PC are non-mutagenic, except peronemin D1 is mutagenic.
Conclusion: The peronemin C1 bioactive compounds from PC have good immunomodulatory potential, effectively inhibiting human IL-6 and TNF-α receptors using in silico approach.
Lehi’s Dream of the Tree of Life: Springboard to Prophecy
Authors: Volluz Corbin T. 
Published: December 2022

Abstract
Lehi’s dream of the tree of life is well known in Latter-day Saint circles. Its relationship to the vision of Nephi (1 Nephi 11-14), however, may not be so well known. This paper examines the proposition that Nephi’s vision was an expansive, prophetic interpretation of Lehi’s dream of the tree of life. An alternate interpretation of Lehi’s dream as a guide to the afterlife is also discussed. Finally, links between Lehi’s dream, the Garden of Eden, and the temple will be considered.
Joseph Smith’s Receipt of the Plates and the Israelite Feast of Trumpets
Authors: Read Lenet Hadley 
Published: December 2022

Abstract
Joseph Smith received the golden plates on the Israelite Day of Remembrance (or Rosh ha-Shanah). Biblical references and interpretation by Jewish sages through the centuries set this day as the day God would remember his covenants with Israel to bring them back from exile. Also called the Feast of Trumpets, on this day ritual trumpet blasts signify the issuance of revelation and a call for Israel to gather for God’s word of redemption. Set at the time of Israel’s final agricultural harvest, the day also symbolizes the Lord’s final harvest of souls. Furthermore, it initiates the completion of the Lord’s time periods, the Days of Awe, and signifies the last time to prepare for final judgment and the Messianic Age. The coming forth of the Book of Mormon is literally fulfilling such prophecies of the day.
A Short Addition to Length: Some Relative Frequencies of Circumstantial Structures
Authors: Stubbs Brian D. 
Published: December 2022

Abstract
In previous articles I have discussed the nature and prominence of certain linguistic structures in the Book of Mormon that are typical of ḥāl-clauses translated from Hebrew or Egyptian. This article compares the frequencies of those structures in three works produced through the instrumentality of Joseph Smith: the Book of Mormon, the Doctrine and Covenants, and History of the Church of Jesus Christ of Latter-day Saints, only the first of which is a translation from an ancient Near Eastern language. The results of this preliminary investigation into styles and these linguistic structures as found in these three works are worth noting.
Peningkatan Kemampuan Membaca Melalui Penggunaan Alfabet Geser pada Murid Tunagrahita
Authors: Meidina Tatiana , Sulasminah Dwiyatmi , Kasmawati St 
Published: December 2022

Penelitian ini bertujuan untuk mendeskripsikan pengaruh penggunaan alphabet geser untuk meningkatkan kemampuan membaca murid tunagrahita kelas dasar II di SLB Negeri 1 Kabupaten Gowa Provinsi Sulawesi Selatan. Jenis penelitian yang digunakan adalah penelitian eksperimen subjek tunggal (single subjek research) dengan desain penelitian A-B-A. Hasil penelitian menunjukkan bahwa penggunaan alphabet geser berpengaruh terhadap peningkatan kemampuan membaca pada murid tunagrahita. Hasil tersebut dibuktikan berdasarkan hasil analisis dalam kondisi: 1) pada fase baseline 1 (A1) kemampuan membaca berada pada kategori sangat rendah, 2) Fase intervensi (B) kemampuan membaca meningkat dan berada pada kategori cukup , 3) fase baseline 2 (A2) kemampuan membaca meningkat dan berada pada kategori tinggi. Berdasarkan analisis antar kondisi tidak terjadi data tumpang tindih sehingga dapat disimpulkan bahwa alphabet geser dapat meningkatkan kemampuan membaca pada murid tunagrahita kelas dasar II di SLB Negeri 1 Kabupaten Gowa Provinsi Sulawesi Selatan.