bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic impact of a 1-week intensive multimodal group program for adolescents with multiple adverse childhood experiences
Authors: Kaliman Perla , Cosín-Tomás Marta , Madrid Andy , Roque-López Susana , Llanez-Anaya Elkin , Papale Ligia A. , Alisch Reid S. , Davidson Richard J. 
Published: June 2022

Abstract
Adverse childhood experiences (ACEs, i.e., abuse, neglect, household dysfunction) are associated with a wide range of long-lasting diseases and shorter life expectancy. We recently described a 1-week residential group program, based on mindfulness training, artistic expression and EMDR group therapy, that significantly reduced PTSD-related symptoms and increased attention/awareness-related outcomes in adolescent girls with multiple ACEs in a randomized controlled study (Roque Lopez et al., 2021). Since epigenetic mechanisms (i.e., DNA methylation) have been associated with the long-lasting effects of ACEs, the present report extends these prior findings by exploring genome-wide DNA methylation changes following the program. Saliva samples from all participants (n=44) were collected and genomic DNA was extracted prior (T1) and following (T2) the intervention. Genome-wide DNA methylation analysis using the MethylationEPIC beadchip array (Illumina) revealed 49 differentially methylated loci (DML; p-value < 0.001; methylation change >10%) that were annotated to genes with known biological processes linked to early childhood adversity (i.e., neural, immune, and endocrine pathways, cancer and cardiovascular disease). DNA sequences flanking these DML showed significant enrichment of transcription factor binding sites involved in inflammation, cancer, cardiovascular disease, and brain development. Methylation changes in SIRT5 and TRAPPC2L genes showed associations with changes in trauma-related psychological measures. Results presented here suggest that this multimodal group program for adolescents with multiple victimization modulates the DNA methylome at sites of potential relevance for health and behavioral disorders associated with ACEs.
Antibiotic Resistance Patterns of Helicobacter pylori Strains Isolated from the Tibet Autonomous Region, China
Authors: Tang Xiaoqiong , Wang Zhonghua , Shen Yalin , Song Xiaona , Benghezal Mohammed , Marshall Barry J. , Tang Hong , Li Hong 
Published: June 2022

Abstract
Background: The prevalence of Helicobacter pylori antibiotic susceptibility in the Tibet Autonomous Region, China is not determined. This study aimed to evaluate the antibiotic resistance patterns of H. pylori isolates there. Results: A total of 153 (38.5%) H. pylori strains were successfully obtained from 397 patients in People's Hospital of Tibet Autonomous Region. The overall resistance rates were as follows: clarithromycin (27.4%), levofloxacin (31.3%), metronidazole (86.2%), amoxicillin (15.6%), tetracycline (0%), furazolidone (0.6%), and rifampicin (73.2%). Only 2.0% of H. pylori isolates were susceptible to all tested antimicrobials, with mono resistance, dual resistance, triple resistance, quadruple resistance, and quintuple resistance being 18.3%, 44.4%, 18.3%, 12.4%, and 4.6%, respectively. The resistance rates to levofloxacin (40.5%) and amoxicillin (21.5%) in strains isolated from female patients were significantly higher than those from male patients (21.6% and 9.5%, respectively). Conclusions: This study demonstrates that H. pylori resistance rates to clarithromycin, levofloxacin, metronidazole, and rifampicin were high, whereas resistance to amoxicillin, tetracycline, and furazolidone was infrequent in Chinese Tibet Autonomous Region. The high resistance to rifampicin warns further investigation of its derivative, rifabutin.
Single Tube Multiplex PCR, Liquid Hybridization, and ELISA (Multiplex NAT-ELISA) for rapid detection of HIV, HBV, and HCV in the window period.
Authors: Sharma Priyanka , Batheja Gunjan , Verma Hriday Narayan , Seth Pradeep 
Published: June 2022

Abstract

Introduction: A molecular assay based on single-tube multiplex PCR, liquid hybridization and indirect ELISA has been designed for rapid detection of HIV-1 & 2, HCV, and HBV in plasma samples (multiplex NAT-ELISA) during the window period of these infections.Methods: Nucleic acid extracted from the virus standards and blood donor plasma samples were amplified by conventional reverse transcription-PCR with a mixture of optimized concentrations of forward and biotinylated reverse primers followed by liquid hybridization of immobilized denatured biotinylated amplicons with digoxigenin-labeled specific probes and indirect ELISA to measure hybridization signals colorimetrically. The performance of Multiplex NAT-ELISA was validated by Analytical Sensitivity, Analytical Specificity, and by the “Accuracy” (Trueness) of the assay.Results: Performance characteristics of the assay were established. Analytical sensitivity (LOD95) was 17 IU/ml (10 geq/ml) for HIV-1) 13 IU/ml (73 geq /ml) for HBV, and 15 IU/ml (66 geq/ml) for HCV. A comparison of the assay with the serological screening tests for HIV/HCV antibodies and HBsAg shows a concordance of 98%. Five serologically negative samples yielded HBV DNA in 4 samples and HCV RNA in one sample by NAT-ELISA.Conclusions: Multiplex NAT-ELISA assay described here is performed on individual donations. The main advantage of the assay is the interdiction of HIV/HCV/HBV virus infections offering a significantly higher sensitivity for detecting blood-borne infections at an affordable cost (less than US$ 5 per sample). Moreover, it has analytical sensitivity that matches that of Procleix Ultrio System and Cobas TaqScreen MPX.
Improving Network Lifetime in Wireless Body Area Network for Health Monitoring
Authors: Chavva Subba Reddy , Edara Deepak Chowdary , Sreeniva Bharath 
Published: June 2022

Abstract
Wireless Body Area Networks (WBANs) is one of the promising technologies for monitoring patient health in real-time. WBANs, routing has a critical part to play to transfer data to sink node or base station. SIMPLE protocol, data transfer to sink node from sensor nodes using multi-hop routing via parent node. The election of this parent node is centered on the distance and residual energy. It has less network lifetime because more nodes die with less time. To increase the network lifetime, Sugeno-Fuzzy Inference System (SuFIS) technique used for election of parent node. The proposed method is to choose the parent node based on residual energy, distance and the alive nodes in every round of the network using SuFIS. Nodes in a network that have the highest sensor value are the parent node of the data flow in that network. The proposed method gives a better network lifetime to compare with existing state-of-art methods SIMPLE and M-ATTEMPT.
Highly anisotropic geometrical Hall effect via f-d exchange fields in doped pyrochlore molybdates
Authors: Ueda Kentaro , Fukuda Hikaru , Tokura Yoshinori , Kaneko Yoshio , Karube Kosuke , Taguchi Yasujiro , Kurihara Ryosuke , Miyake Atsushi , Tokunaga Masashi 
Published: June 2022

Abstract
When a conduction electron couples with a non-coplanar localized magnetic moment, the real-space Berry curvature is exerted to cause the geometrical Hall effect, which is not simply proportional to the magnetization. So far, it has been identified in the case mostly where the non-coplanar magnetic order is present on the sublattice of conduction electrons. Here, we demonstrate that the geometrical Hall effect shows up even without long-range magnetic order of conduction electrons, as induced by non-coplanar exchange fields from the localized magnetic moments, in hole-doped pyrochlore molybdates. We find that the geometrical Hall effect is markedly anisotropic with respect to the applied magnetic field direction, which is in good accordance with the field-dependent magnitude and sign change of the real-space scalar spin chirality of local Tb moments. These results may facilitate the understanding of emergent electromagnetic responses induced by the Kondo-like coupling between conduction electrons and local spins in a broad material class.
Serum proteomic profiling reveals MTA2 and AGO2 as novel prognostic biomarkers associated with disease activity and adverse outcomes in multiple myeloma
Authors: Apipongrat Dollapak , Roytrakul Sittiruk , Prayongratana Kannadit , Intharanut Kamphon , Laoruangroj Chonlada , Silpsamrit Panachai , Nathalang Oytip 
Published: June 2022

Abstract
Background: Multiple myeloma (MM) is an incurable plasma cell malignancy. Several studies have demonstrated usefulness of the proteomic approach to identify potential biomarkers in various malignancies; however, data in patients with MM are limited. Identifying novel biomarkers that can reliably indicate disease activity, progression and patient outcomes remains a major challenge. This study aimed to identify potential prognostic biomarkers corresponding to MM disease activity and evaluate their impact on patient outcomes.Methods: Serum proteomic profiles of patients and age-matched controls were analyzed. The expression of the candidate proteins was confirmed using an NCBI Gene Expression Omnibus dataset. In the verification and validation phases, the concentration of the candidate biomarkers was measured using an enzyme-linked immunosorbent assay. In addition, the association of the proposed biomarkers with clinical outcomes was assessed.Results: Focused on newly diagnosed MM and relapsed/refractory MM, 23 upregulated and 15 downregulated proteins were identified. The top two proteins, metastasis-associated protein-2 (MTA2) and argonaute-2 (AGO2), were selected for further verification and validation studies as candidate biomarkers. We found that both MTA2 and AGO2 showed significantly higher levels in the disease active states compared with the remission states (p < 0.001). Regardless of the patient treatment profile, high MTA2 levels were associated with shorter progression-free survival (PFS, p = 0.008, HR = 2.16, 95% CI: 1.01-5.11). Conversely, high AGO2 levels were associated with IgG and kappa light-chains isotypes and bone involvement features (p < 0.05), and associated with prolonged time to response (TTR, p = 0.046, HR = 2.92, 95% CI: 1.02-8.40) among patients with MM.Conclusion: MTA2 and AGO2 proteins were firstly identified as potential serum biomarkers providing diagnostic and prognostic values. Elevated levels of those biomarkers were associated with adverse outcomes in patients with MM. Implementing serum MTA2 and AGO2 may be beneficial due to its simplicity, shortening turn-around time, being less painful, and able to perform in a routine laboratory.
A novel target for cancer therapy on the RNA stimulation by FUBP1- interacting repressor (FIR) via TFIIH/P62
Authors: Kitamura Kouichi , Hoshino Tyuji , Okabe Atsushi , Fukuyo Masaki , Rahmutulla Bahityar , Tanaka Nobuko , Kobayashi Sohei , Tanaka Tomoaki , Shida Takashi , Ueda Mashiro , Minamoto Toshinari , Matsubara Hisahiro , Kaneda Atsushi , Matsushita Kazuyuki 
Published: June 2022

Abstract
This study indicated the novel impaired mechanism of ribosomal RNA (rRNA) transcription that causes cancer development. Human transcription factor IIH (TFIIH) is an essential factor with a seven-subunit core, including the P62 subunit, and is pivotal for DNA repair and transcription. Three RNA polymerases (RNAPs), RNAPI, II and III, possess a common RPB6 subunit that interacts with P62 of TFIIH. The FUBP1-interacting repressor (FIR) is a transcriptional suppressor of c-myc via suppressing DNA helicase activity of TFIIH. This study showed that FIR contains a highly conserved acidic string region, like the region of the RPB6 subunit of RNAPI. Knockdown of FIR by siRNA suppressed P62 expression in vivo in cancer cells. The isothermal titration calorimetry (ITC) measurements revealed the molecular interaction between the authentic FIR rather than FIRΔexon2, a dominant-negative splicing form of FIR that lacks the transcriptional repression domain exon2, and P62. Furthermore, knockdown of FIR and FIRΔexon2 by siRNA led to dynamic changes in ribosomal RNA (rRNA) expression assessed by RNA-seq analysis. A qRT-PCR analysis confirmed that FIR and FIRΔexon2 significantly changed the expression of numerous rRNAs on specific chromosomes. Moreover, small molecular chemical compounds against FIR and FIRΔexon2 were screened among 23,275 chemicals by Natural Product Depository (NPDepo) Array at RIKEN (Japan). Among them, BK697 that potentially binds FIR and FIRΔexon2 significantly inhibited tumor cell growth with the suppression of ribosomal proteins, FIR, and FIRΔexon2 expression. In summary, FIR and FIRΔexon2 provide a novel insight into cell proliferation by stimulating dynamic ribosome biosynthesis and a hint of therapeutic strategy.
K fragment, a PCR-based vector for antibiotic resistance gene hunting
Authors: Salih Hanife , Oryaşın Erman , Bozdoğan Bülent 
Published: June 2022

Abstract
Antimicrobial resistance (AMR) is an important health issue in the world. To evaluate and predict, known and unknown resistance genes that are responsible for AMR should be identified and characterized. Unlike known genes, unknown genes are difficult to detect. In this study, we generated the plasmid of K fragment (pKF) from pUC19 vector by removing multiple cloning site (MCS) and adding Prom-RBS sequence. pKF was used for amplification of K fragment that contains RBS and promoter at both directions and plasmid origin of replication. The functionality of added Prom-RBS sequence and K fragment as a cloning vector was tested by cloning chloramphenicol resistance gene amplicon and erythromycin resistance gene from genomic DNA, respectively. Cloning of them confirmed the usability of this newly developed cloning method with K fragment. K fragment is a PCR based and antibiotic resistance gene-free cloning vector. It allows cloning of resistance genes at all orientations just by changing restriction enzymes for primers and fragmentation. This method enables the microbiological and molecular characterization of cloned genes. We expect the use of K fragment for cloning of resistance genes from different environments without cultivation. It will contribute to fighting AMR by identification of resistance genes before their dissemination.
Thematic analysis of smokers’ attitudes towards lung cancer screening using spiral CT scanning
Authors: Ahmed Farheen 
Published: June 2022

Abstract
Background
Spiral CT scanning can be used to screen current smokers who may be at higher risk of developing lung cancer. However, there is little understanding on whether smokers would be more likely to attend for lung cancer screening if this option were available.
Methods
Semi-structured interviews with nine at-risk smokers were conducted.
Results
The results of the thematic analysis showed that though most participants displayed a positive attitude towards lung cancer screening, they seemed to identify more with the barriers than the facilitators and would therefore not attend for screening. Two themes were identified: (1) Smokers’ beliefs and misconceptions and (2) Fear of screening outcome. These encompassed participants lower cancer risk perceptions, fatalistic beliefs, lack of trust in health professionals and their fears associated with being screened and receiving a cancer diagnosis.
Conclusion
Therefore, in order to maximise screening uptake, greater focus is needed on understanding how these barriers affect smokers’ decision-making processes before investment into a national screening programme is made.
The implementation of anthropometric measurement in designing the ergonomics work furniture
Authors: Silviana Silviana , Hardianto Andy , Hermawan Dadang 
Published: June 2022

The productivity of an employee has a crucial role for the company. The higher the level of productivity, the higher the profit they can provide for the company. Ergonomic furniture is one of the supporting examples in increasing employee productivity. Designing ergonomic furniture is the effort taken by the company to boost the employee's productivity. The ergonomic furniture can provide comfort to the employee and minimize the occurrence of Musculoskeletal Disorders (MSDs). The provided coziness will increase the level of concentration so that the performance of the employee will be increased too. The existence of ergonomic furniture will offer benefits to the company. Therefore, companies are required to provide adequate facilities and infrastructure to support employee activities. One of the company's facilities is a table and chairs. Tables and chairs must be designed ergonomically, considering that both facilities will continually be used within 8 hours per day. This research aims to utilize anthropometric measurement as a design tool for ergonomic work furniture. The anthropometric measurement was conducted on 85 employees and used 11 body dimensions as a parameter. These body dimensions consist of a supine hand, sitting, forward pinch-grip reach, elbow height, shoulder width, pelvic width, buttock-knee length, knee height, shoulder height, sitting, length of elbow to toe, thigh clearance, and palm width. The 5th percentile was used for four dimensions in this research, while the rest used 95th. The results of this research are expected to minimize the possibility of various health problems caused by the un-ergonomic working furniture so that the employee's performance will be improved too
Performance assessment of sewage treatment plants using compliance index
Authors: Ramkumar D. , Jothiprakash V. , Patil B. N. 
Published: June 2022

Abstract
The aim of this study is to infer the performance assessment of sewage treatment plants (STPs) using the compliance index (CI). The methodology includes three steps, (1) estimation of performance indicators from the quality test, parameter test, and conformity test, (2) hypothesis testing of non-conformity, and (3) hypothesis testing using the non-parametric test. The CI integrates the results of the above nine tests through a scoring system based on individual performance. The study area, Navi Mumbai Municipal Corporation (NMMC), India, treats its domestic sewage through seven centralized STPs located in different zones. The influent and effluent parameters, such as flow rate, biochemical oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids (TSS), pH, dissolved oxygen (DO), and temperature, were studied in this work. The removal efficiency (RE) analysis shows the efficiency of BOD, COD, and TSS varies between 96.67 and 97.30%, 84.55 and 89.70%, and 94.04 and 95.81%, respectively, and indicates that all STPs are performing well. The proposed CI infers that all STPs perform well and comply with NMMC standards, except one STP, namely Airoli. Interestingly, RE analysis shows that Airoli STP's BOD is the third-best compared to all other STPs. This study shows that the CI method can infer the performance of STPs better than RE and help the administrators to plan, execute, operate, and maintain STPs to achieve total sanitation in megacities.
A framework for optimal rank identification of resource management systems using probabilistic approaches in analytic hierarchy process
Authors: Gorripati Ravi , Thakur Mainak , Kolagani Nagesh 
Published: June 2022

Abstract
A resource management system is likely to succeed if stakeholders get involved in analyzing and choosing from the alternatives. The present work deals with multi-criteria decision models to evaluate rain water harvesting (RWH) structures. Standard practice is to acquire the weights for criteria from stakeholders using analytic hierarchy process (AHP) to predict the RWH structures' performance and rank them. Challenges in this process are that the data collection is laborious and time-consuming, considers limited stakeholders' opinions, and suffers from lower confidence factors. This work proposes a probabilistic approach to AHP using Monte Carlo simulation (MCS) to model uncertainty. The proposal is to collect multiple assessments instead of a single judgment from knowledgeable stakeholders (KSH) with customized questionnaires and to compute the relative importance of criteria using pairwise comparisons. Stochastically similar assessments within the range of these samples are then generated using different distribution functions to compute the performance of the RWH structures. The computed performance correlated well with common stakeholders' (CSH) opinions in the case study involving 10 existing RWH structures with seven different criteria, for all the distributions. The mean relative error with the proposed method is approximately 21% less than the existing point estimate method.
Water quality monitoring using remote sensing, Lower Manyame Sub-catchment, Zimbabwe
Authors: Muhoyi H. , Gumindoga W. , Mhizha A. , Misi S. N. , Nondo N. 
Published: June 2022

Abstract
The Lower Manyame Sub-catchment (LMS) is one of the most heavily polluted in Zimbabwe. Its waters are valuable for irrigation, domestic and industrial purposes. LMS has serious eutrophication problems emanating from human activities within it, and lakes Manyame and Chivero upstream. Data collected from October 2018 to April 2019 were used to test an integrated methodology based on field measurements and remote sensing. This study illustrates the production of multitemporal spatialised maps of total suspended solids (TSS), chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) concentrations from satellite data acquired from Sentinel-2. The analysis confirmed the pollution (eutrophic and organic matter) status of LMS water, for the period considered by this research. As a result, careful land planning must be done through the joint operation of local authorities, regional agencies and regional institutions, since Manyame River is a tributary of the Zambezi River (a transboundary river).
Histomorphological Evaluation of Non-Neoplastic Lesions of Uterine Cervix and a Correlation of the Lesion with the Clinical Factors
Authors: Periasamy Panneerselvam , Dhanaraj Prem Charles , Ulaganathan Manohar , Renuka Remya Rajan , Pandiyan Rajesh 
Published: June 2022

Aim:
This study investigates the prevalence of non-malignant lesions of the cervix
among various biopsy samples.

Methods:
This case study consists of 50 cases of cervical biopsy over almost two years. The case
history and clinical details of the patients were obtained.

Results:
60% of the cases that participated in this study reported white discharge per vaginum as a
common clinical symptom. 4 cases (8%) showed koilocytic changes specific to the human papillomavirus
during the study. Only 2% of the non-specific cervicitis showed lymphoid aggregates.
Endocervical changes projected papillary endocervicitis with 9 cases (18%), squamous metaplasia
with 7 cases (14%), and nabothian follicle cyst with 3 cases (6%).

Conclusion:
It has been concluded that 50 cases were studied histologically, which had adequate
representation of both ecto and endocervical tissue. Moreover, 31-40 years of age of patients
showed the highest percentage of non-neoplastic lesions of the cervix when compared to other age
groups.