bluuebunny/crossref_metadata_2025_split huggingface dataset:
Handheld pH‐Meter‐based Electrochemical Aptasensing of Carcinoembryonic Antigen on Multifuctional Magnetic Beads
Authors: Chen Shaobo , Lin Shujin , Han Xiao , Han Xianlin 
Published: March 2022

AbstractMethods derived from immunoassays and aptasensors have been established for monitoring of carcinoembryonic antigen (CEA) in biological fluids, but most involved complex procedure and various reactions. We designed an uncomplicated and convenient electrochemical aptasensing platform for the quantitative monitoring of CEA on multifunctionalized magnetic beads with a handheld pH meter as the read‐out. Initially, CEA‐specific aptamers were covalently conjugated to magnetic beads, and then hybridized with glucose oxidase‐labeled complementary strands to construct multifunctional nanoprobes. After addition of target CEA, the analyte interacts with the corresponding aptamer and then release the complementary strand. After magnetic separation, the released glucose oxidase‐labeled complementary strands hydrolysed glucose, thereby leading to a change in pH of the detection solution, which was quantitatively monitored on a hand‐held pH meter. Under the optimum conditions, the aptasensor based on pH meter exhibited well responses relative to CEA concentrations inside the dynamic range between 0.01 and 100 ng mL−1 and detection limit at least 9.3 pg mL−1. Good duplicability, high specificity and ability for long‐term stable storage are obtained for the as‐prepared aptasensors. Additionally, human serum specimens were determined through pH‐meter‐based aptasensors, and the results closely matched the commercial enzyme‐linked immunosorbent assay (ELISA) of human CEA.
Gender expression is associated with selection of uterine preservation or hysterectomy for pelvic organ prolapse surgery: Novel methodology for sex‐ and gender‐based analysis in gynecologic research
Authors: Cummings Shannon , Ramage Kaylee , Scime Natalie V. , Ahmed Sofia B. , Brennand Erin A. 
Published: March 2022

AbstractObjectiveTo explore whether patient characteristics were associated with gender expression, and to further determine impact of gender expression on patient selection of hysterectomy or uterine‐preservation in pelvic organ prolapse (POP) surgery.MethodsWithin a prospective cohort, a self‐reported gender expression tool classified patients as expressing gender polar (i.e., reporting only feminine traits) or non‐polar gender scores (i.e., reporting feminine and masculine traits). Multivariate modeling explored associations of gender expression with traditional socio‐demographic variables, and with selection of hysterectomy or uterine‐preserving surgery. Descriptive statistics of socio‐demographic variables were reported by frequency, proportion and mean (SD).Results177 participants completed the gender score questionnaire. Overall, the sample had a more feminine gender expression with the majority of respondents classified as gender polar (67.23%, n = 119). Participants with non‐polar gender scores were 2.53 times (95% 1.05–6.09) more likely to choose uterine preservation versus hysterectomy‐based surgery. Gender polarity was weakly associated with age, but no other sociodemographic variables.ConclusionGender expression is not tightly associated with socio‐demographic variables, and is thus a novel measurement in gynecologic research. Gender polarity appears to be associated with choice to undergo hysterectomy. Further research is required to understand this relationship and implications in clinical outcomes.
Machine learning based on automated breast volume scanner (ABVS) radiomics for differential diagnosis of benign and malignant BI‐RADS 4 lesions
Authors: Wang Shi‐jie , Liu Hua‐qing , Yang Tao , Huang Ming‐quan , Zheng Bo‐wen , Wu Tao , Han Lan‐qing , Zhang Yong , Ren Jie 
Published: March 2022

AbstractBI‐RADS category 4 represents possibly malignant lesions and biopsy is recommended to distinguish benign and malignant. However, studies revealed that up to 67%–78% of BI‐RADS 4 lesions proved to be benign, but received unnecessary biopsies, which may cause unnecessary anxiety and discomfort to patients and increase the burden on the healthcare system. In this prospective study, machine learning (ML) based on the emerging breast ultrasound technology‐automated breast volume scanner (ABVS) was constructed to distinguish benign and malignant BI‐RADS 4 lesions and compared with different experienced radiologists. A total of 223 pathologically confirmed BI‐RADS 4 lesions were recruited and divided into training and testing cohorts. Radiomics features were extracted from axial, sagittal, and coronal ABVS images for each lesion. Seven feature selection methods and 13 ML algorithms were used to construct different ML pipelines, of which the DNN‐RFE (combination of recursive feature elimination and deep neural networks) had the best performance in both training and testing cohorts. The AUC value of the DNN‐RFE was significantly higher than less experienced radiologist at Delong's test (0.954 vs. 0.776, p = 0.004). Additionally, the accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the DNN‐RFE were 88.9%, 83.3%, 95.2%, 83.3%, and 95.2%, which also significantly better than less experienced radiologist at McNemar's test (p = 0.043). Therefore, ML based on ABVS radiomics may be a potential method to non‐invasively distinguish benign and malignant BI‐RADS 4 lesions.
Processes of frugal social innovation: Creative approaches in underserved South African communities
Authors: Lorini Maria Rosa , Ngwenyama Ojelanki , Chigona Wallace 
Published: March 2022

AbstractThe article presents three case studies of frugal social innovation developed by groups of citizens from underserved communities of Cape Town, South Africa. The processes are analyzed to highlight how innovation emerged. Three factors were crucial: lack of resources, social transformation goals, and flexibility of the approach to technologies. Their combination allowed for creativity and inclusivity to become the drivers of the processes. The information and communication technology outcomes are innovative in the context and for the participants. More innovative are the processes, which maintained a high level of participation, a collective collaboration and a focus on the social transformative impact of the digital solutions. Furthermore, while much of the literature on frugal and social innovation has a business perspective whereby users are referred to as customers, the cases present community groups as innovators. This approach contributes to the development of a theory, which expands existing ones on frugal and social innovation. The principles derived from the analysis represent the contribution to practice in the ICT4D domain. They show how in a space with limited technical and procedural knowledge, it is possible to reduce the blinders toward innovation and operate in an ecosystem where participation, inclusion, and growth develop.
Antagonism Between PEDF and TGF-β Contributes to Type VI Osteogenesis Imperfecta Bone and Vascular Pathogenesis
Authors: Kang Heeseog , Aryal AC Smriti , Barnes Aileen M , Martin Aline , David Valentin , Crawford Susan E , Marini Joan C 
Published: March 2022

ABSTRACT
Osteogenesis imperfecta (OI) is a heterogeneous genetic disorder of bone and connective tissue, also known as brittle bone disease. Null mutations in SERPINF1, which encodes pigment epithelium-derived factor (PEDF), cause severe type VI OI, characterized by accumulation of unmineralized osteoid and a fish-scale pattern of bone lamellae. Although the potent anti-angiogenic activity of PEDF has been extensively studied, the disease mechanism of type VI OI is not well understood. Using Serpinf1(−/−) mice and primary osteoblasts, we demonstrate that loss of PEDF delays osteoblast maturation as well as extracellular matrix (ECM) mineralization. Barium sulfate perfusion reveals significantly increased vessel density in the tibial periosteum of Serpinf1(−/−) mouse compared with wild-type littermates. The increased bone vascularization in Serpinf1(−/−) mice correlated with increased number of CD31(+)/Endomucin(+) endothelial cells, which are involved in the coupling angiogenesis and osteogenesis. Global transcriptome analysis by RNA-Seq of Serpinf1(−/−) mouse osteoblasts reveals osteogenesis and angiogenesis as the biological processes most impacted by loss of PEDF. Intriguingly, TGF-β signaling is activated in type VI OI cells, and Serpinf1(−/−) osteoblasts are more sensitive to TGF-β stimulation than wild-type osteoblasts. TGF-β stimulation and PEDF deficiency showed additive effects on transcription suppression of osteogenic markers and stimulation of pro-angiogenic factors. Furthermore, PEDF attenuated TGF-β-induced expression of pro-angiogenic factors. These data suggest that functional antagonism between PEDF and TGF-β pathways controls osteogenesis and bone vascularization and is implicated in type VI OI pathogenesis. This antagonism may be exploited in developing therapeutics for type VI OI utilizing PEDF and TGF-β antibody. © 2022 American Society for Bone and Mineral Research (ASBMR). This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
3D Image Registration Marginally Improves the Precision of HR-pQCT Measurements Compared to Cross-Sectional-Area Registration in Adults With Osteogenesis Imperfecta
Authors: Hosseinitabatabaei Seyedmahdi , Mikolajewicz Nicholas , Zimmermann Elizabeth A , Rummler Maximilian , Steyn Beatrice , Julien Catherine , Rauch Frank , Willie Bettina M 
Published: March 2022

ABSTRACT
Repositioning error in longitudinal high-resolution peripheral-quantitative computed tomography (HR-pQCT) imaging can lead to different bone volumes being assessed over time. To identify the same bone volumes at each time point, image registration is used. While cross-sectional area image registration corrects axial misalignment, 3D registration additionally corrects rotations. Other registration methods involving matched angle analysis (MA) or boundary transformations (3D-TB) can be used to limit interpolation error in 3D-registering micro-finite-element data. We investigated the effect of different image registration methods on short-term in vivo precision in adults with osteogenesis imperfecta, a collagen-related genetic disorder resulting in low bone mass, impaired quality, and increased fragility. The radii and tibiae of 29 participants were imaged twice on the same day with full repositioning. We compared the precision error of different image registration methods for density, microstructural, and micro-finite-element outcomes with data stratified based on anatomical site, motion status, and scanner generation. Regardless of the stratification, we found that image registration improved precision for total and trabecular bone mineral densities, trabecular and cortical bone mineral contents, area measurements, trabecular bone volume fraction, separation, and heterogeneity, as well as cortical thickness and perimeter. 3D registration marginally outperformed cross-sectional area registration for some outcomes, such as trabecular bone volume fraction and separation. Similarly, precision of micro-finite-element outcomes was improved after image registration, with 3D-TB and MA methods providing greatest improvements. Our regression model confirmed the beneficial effect of image registration on HR-pQCT precision errors, whereas motion had a detrimental effect on precision even after image registration. Collectively, our results indicate that 3D registration is recommended for longitudinal HR-pQCT imaging in adults with osteogenesis imperfecta. Since our precision errors are similar to those of healthy adults, these results can likely be extended to other populations, although future studies are needed to confirm this. © 2022 American Society for Bone and Mineral Research (ASBMR).
Structure, charge density, and Hirshfeld surface analysis of proton transfer complex 2‐amino‐4‐methylpyridinium 2‐(3‐methylphenyl)‐acetate
Authors: Anzline Chellam , Israel Samuel , Sujatha Kanagasabapathi , Sheeba Rajan Arul Jebamani Raja 
Published: March 2022

AbstractThe compounds m‐Tolylacetic acid and 2‐amino‐4‐Picoline were used as a precursor to synthesize the molecular complex 2‐amino‐4‐methylpyridinium 2‐(3‐methylphenyl)‐acetate by means of proton transfer. High‐resolution X‐ray diffraction data were collected for the crystallized complex at 100 K. The structure was solved spherically and further an aspherical model refinement was performed using Hansen and Coppens multipole formalism. Topological properties using AIM analysis were carried out for the title compound to understand the atom–atom interactions and the strength of the bond. The structure exhibits interesting patterns of N–H…O and C–H…O hydrogen bonds. A pair of N–H…O hydrogen bonds links the anion and cation of the molecular complex generating an R22(8) ring motif. These ring motifs are linked to adjacent anions and cations through another intermolecular N–H…O hydrogen bond generating a bifurcated R22(8)ring motif. Hirshfeld surface analysis was carried out to understand the types of inter‐molecular interactions and their strengths.
Long non‐coding RNA screening and identification of potential biomarkers for type 2 diabetes
Authors: Ma Qi , Wang Li , Wang Zhiqiang , Su Yinxia , Hou Qinqin , Xu Qiushuang , Cai Ren , Wang Tingting , Gong Xueli , Yi Qizhong 
Published: March 2022

AbstractBackgroundTo investigate new lncRNAs as molecular markers of T2D.MethodsWe used microarrays to identify differentially expressed lncRNAs and mRNAs from five patients with T2D and paired controls. Through bioinformatics analysis, qRT‐PCR validation, ELISA, and receiver operating characteristic (ROC) curve analysis of 100 patients with T2D and 100 controls to evaluate the correlation between lncRNAs and T2D, and whether lncRNAs could be used in the diagnosis of T2D patients.ResultsWe identified 68 and 74 differentially expressed lncRNAs and mRNAs, respectively. The top five upregulated lncRNAs are ENST00000381108.3, ENST00000515544.1, ENST00000539543.1, ENST00000508174.1, and ENST00000564527.1, and the top five downregulated lncRNAs are TCONS_00017539, ENST00000430816.1, ENST00000533203.1, ENST00000609522.1, and ENST00000417079.1. The top five upregulated mRNAs are Q59H50, CYP27A1, DNASE1L3, GRIP2, and lnc‐TMEM18‐12, and the top five downregulated mRNAs are GSTM4, PODN, GLYATL2, ZNF772, and CLTC. Examination of lncRNA‐mRNA interaction pairs indicated that the target gene of lncRNA XR_108954.2 is E2F2. Multiple linear regression analysis showed that XR_108954.2 (r = 0.387, p < 0.01) and E2F2 (r = 0.368, p < 0.01) expression levels were positively correlated with glucose metabolism indicators. Moreover, E2F2 was positively correlated with lipid metabolism indicators (r = 0.333, p < 0.05). The area under the ROC curve was 0.704 (95% CI: 0.578–0.830, p = 0.05) for lncRNA XR_108954.2 and 0.653 (95% CI: 0.516–0.790, p = 0.035) for E2F2.ConclusionsThis transcriptome analysis explored the aberrantly expressed lncRNAs and identified E2F2 and lncRNA XR_108954.2 as potential biomarkers for patients with T2D.
The prognostic effect of PNN in digestive tract cancers and its correlation with the tumor immune landscape in colon adenocarcinoma
Authors: Zhang Hui , Jin Ming , Ye Meng , Bei Yanping , Yang Shaohui , Liu Kaitai 
Published: March 2022

AbstractBackgroundThe present study investigated the expression, mutation, and methylation profile of PNN and its prognostic value in digestive tract cancers. The disparities in signaling pathways and the immune landscape in colon adenocarcinoma (COAD) based on PNN expression were specifically explored.MethodsThe expression, mutation, methylation levels of PNN, and survival data in esophageal cancer, gastric adenocarcinoma, COAD, and rectal adenocarcinoma were evaluated using several bioinformatic databases. Gene Ontology (GO) enrichment analysis and gene set enrichment analysis (GSEA) were performed to investigate the enriched biological functions and pathways in COAD. Several acknowledged bioinformatic algorithms were employed to assess the correlation between PNN expression and the tumor immune landscape in COAD.ResultsPNN was upregulated and remarkably related to tumor stage in digestive tract cancers. High expression of PNN was positively associated with poor progression‐free survival and overall survival time, specifically in COAD. PNN expression was identified as an independent prognostic factor in COAD. GO and GSEA analyses revealed that PNN participates in multiple biological processes underlying carcinogenicity in COAD. Further investigation showed that PNN expression was significantly associated with tumor‐infiltrating immune cells, immune cell functions, and several immune checkpoints in COAD. The PNN low expression group had a lower tumor immune dysfunction and exclusion (TIDE) score and a higher immunophenoscore (IPS), indicating a better response to immunotherapy.ConclusionPNN was highly expressed in digestive tract cancers and could act as an independent prognostic factor and a response predictor for immunotherapy in COAD.
Pedagogical stance in mentalization‐based treatment
Authors: Folmo Espen J. , Langjord Tuva , Myhrvold Nini C. S. , Stänicke Erik , Lind Majse , Kvarstein Elfrida H. 
Published: March 2022

AbstractBackgroundA common aspect of evidence‐based treatments for people with borderline personality disorder (BPD) is pedagogical interventions and formats. In mentalization‐based treatment (MBT) the introductory course has a clear pedagogical format, but a pedagogical stance is not otherwise defined.MethodsTreatment integrity was quantitatively assessed in a sample of 346 individual MBT sessions. Nine group sessions and 24 individual MBT sessions were qualitatively subjected to interpretative phenomenological analysis (IPA).ResultsThe dominating intervention type was MBT Item 16—therapist checking own understanding (31% of the interventions). IPA unveiled the following: (1) a pervasive, but hidden/implicit psychopedagogical agenda, (2) psychopedagogical content seemed precious for the patients, and (3) four tentative strategies for pedagogical interventions in MBT (a) independent reasoning; (b) epistemic trust; (c) mental flexibility; and (d) application of verified insights, knowledge, or strategies.ConclusionDevelopment and clarification of the pedagogical stance in MBT could further improve the quality of therapists' interventions.
Influence of social media platforms in dental education and clinical practice: A cross‐sectional survey among dental trainees and professionals
Authors: Kurian Nirmal , Varghese Ida Ann , Cherian Joe Mathew , Varghese Vinaya Susan , Sabu Aneeta Mary , Sharma Priyanka , Varghese Mevin George 
Published: March 2022

AbstractPurpose/objectivesSocial media platforms (SMPs) have become a popular portal for the acquisition and dissemination of dentistry‐related information. The study aims to identify the pattern and influence SMPs for education and practice among dental trainees and professionals.Method and materialsA cross‐sectional study involving dental trainees and clinical professionals was conducted based on a structured 20‐item survey instrument among dental trainees and professionals. The validated questionnaire was circulated among six dental training institutions in India through the internet‐based application Google form and achieved a response rate of 60%. The questionnaire focused on identifying the usage of social media in disseminating dentistry‐related content. The data from responders were extracted, sorted, and analyzed for descriptive analysis of various factors.ResultThe survey revealed the most common SMP used for dentistry‐related content as YouTube. The second most commonly used SMP was Facebook for professionals whereas it was Instagram for younger trainees. There was a highly significant difference between professionals and trainees concerning their sources of new information and the most commonly used SMP for dental education (p < 0.001).ConclusionsThere is a growing consensus among young dental trainees and professionals on SMPs being an effective open access knowledge center. The survey results call for the need for a scientific journal and dental schools to have verified pages in social media to act as flagbearers of evidence‐based dental practices. The potentials of SMPs in providing open access information collection to wider viewership across the globe and being a digital archive in attractive audio‐visual forms can be used to strengthen the conventional educational model and improvise clinical practice.
Diastolic Function Assessment of Left and Right Ventricles by MRI in Systemic Sclerosis Patients
Authors: Mousseaux Elie , Agoston‐Coldea Lucia , Marjanovic Zora , Baudet Mathilde , Reverdito Guillaume , Bollache Emilie , Kachenoura Nadjia , Messas Emmanuel , Soulat Gilles , Farge Dominique 
Published: March 2022

BackgroundHeart involvement is frequent although often clinically silent in systemic sclerosis (SSc) patients. Early identification of cardiac involvement can be improved by noninvasive methods such as MRI, in addition to transthoracic echocardiography (TTE).PurposeTo assess the ability of phase‐contrast (PC)‐MRI to detect subclinical left (LV) and right (RV) ventricular diastolic dysfunction in SSc patients.Study TypeProspective.PopulationThirty‐five consecutive SSc patients (49 ± 14 years) and 35 sex‐ and age‐matched healthy controls (48.6 ± 13.5 years) who underwent TTE and MRI in the same week.Field Strength/Sequence5 T/PC‐MRI using a breath‐hold velocity‐encoded gradient echo sequence.AssessmentLV TTE (E/E′) and LV and RV PC‐MRI indices of diastolic function (LV early and late transmitral [EM, EfM, AM, AfM] and RV transtricuspid [ET, EfT, AT, AfT] peak filling flow velocities and flow rates, as well as LV [] and RV [] peak longitudinal myocardial velocities during diastole) were measured.Statistical TestsTwo‐tailed t‐test, Wilcoxon test, or Fischer test for comparison of variables between SSc and healthy control groups; sensitivity, specificity, receiver‐operating‐characteristic (ROC) area under the curve (AUC) to assess discriminative ability of variables. A P‐value <0.05 was considered statistically significant.ResultsTTE LV E/E′ and MRI EM/ and ET/ were significantly higher in SSc patients than in controls (8.27 ± 1.25 vs. 6.70 ± 1.66; 9.43 ± 2.7 vs. 6.51 ± 1.50; 6.51 [4.70–10.40] vs. 4.13 [3.22–5.75], respectively) and separated SSc patients and healthy controls with good sensitivity (68%, 71%, and 80%), specificity (85%, 94%, and 62%), and AUC (0.787, 0.807, and 0.765). LV EfM was significantly higher in SSc patients than in controls (347.1 ± 113.7 vs. 284.7 ± 94.6) as RVAfT (277 [231–355] vs. 220 [154–253] mL/sec) with impaired relaxation pattern (EfT/AfT, 0.95 [0.87–1.21] vs. 1.12 [0.93–1.47]).Data ConclusionMRI was able to detect LV and RV diastolic dysfunction in SSc patients with good accuracy in the absence of LV systolic dysfunction at echocardiography. Use of MRI can allow to better assess the early impact of myocardial fibrosis related to SSc.Level of Evidence1Technical Efficacy Stage2