bluuebunny/crossref_metadata_2025_split huggingface dataset:
Owner‐reported outcome measures in veterinary care for companion animal orthopedic patients: An international online survey of veterinarians' expectations and practices
Authors: Radke Heidi , Zhu Tracy Y. , Knoll Christian , Allen Matthew J. , Joeris Alexander 
Published: June 2022

AbstractObjectiveTo report veterinarians' familiarity with and perceptions of owner/observer‐reported outcome measures (OROMs) and their applications in routine clinical practice.Study designCross‐sectional online survey.Animals or sample populationA total of 441 veterinarians with a caseload of companion animal orthopedic patients.MethodsRespondents answered questions regarding their familiarity with and application of OROMs. Respondents provided opinions on statements related to reasons for using or not using OROMs in routine clinical practice, prerequisites to implement OROMs in routine clinical practice, and whether they would implement OROMs in routine clinical practice if adequate tools/technologies were available.ResultsMost (293/441, 66.4%) respondents felt familiarity with at least one OROM. Only 17.5% (77 out of the 440 answering the question about the application of OROMs) applied OROMs in routine clinical practice. The two main reasons for not using OROMs in routine clinical practice were the lack of opportunity/feasibility and the lack sufficient information/knowledge/experiences. User‐friendliness, time efficiency, and interpretability were considered the most important prerequisites for implementing OROMs in routine clinical practice. If adequate tools/technologies were available, 266/439 (60.6%) respondents indicated that they would definitely implement OROMs in routine clinical practice.ConclusionAlthough most respondents were familiar with at least one OROM, fewer than one‐fifth applied them in routine clinical practice. Most respondents were willing to implement OROMs in routine clinical practice if adequate tools/technologies were available.Clinical significanceOur results justify further investigations to improve the application of OROMs in routine clinical practice in veterinary care of companion animal orthopedic patients.
The promise of small particles: extracellular vesicles as biomarkers in liver pathology
Authors: Srinivas Akshatha N. , Suresh Diwakar , Kaur Savneet , Kumar Divya P. 
Published: June 2022

AbstractExtracellular vesicles (EVs) are nanoscopic packages that are heterogeneous and bona fide players in hepatic physiology and pathology as they are involved in intercellular communication. EVs carrying bioactive cargoes rich in lipids, proteins or nucleic acids are implicated in the onset and progression of liver diseases. Liver pathology using liver biopsy has been assessed for several intricate conditions such as viral hepatitis, alcoholic and non‐alcoholic fatty liver disease, hepatic malignancies and drug‐induced liver injury. The lacunae, however, lie in early diagnosis and timely treatment of the above conditions, underscoring the need for non‐invasive, accurate diagnostic tools that could replace the gold standard method of tissue biopsy. In this regard, EVs have emerged as promising candidates that could serve as potential biomarkers. In the last two decades, EVs, owing to their multifaceted charm in bringing out cell‐free therapeutic responses and the ability of their cargoes to be applied to novel biomarkers, have drawn the great attention of researchers with the advancement and clinical application of liquid biopsy. In this review, we recapitulate the role of EVs and provide insights into the promising role of these small packages as biomarkers in liver pathology.
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Research on Multiobjective Optimization Design of Meshing Performance and Dynamic Characteristics of Herringbone Gear Pair Under 3D Modification
Authors: Li Zhibin , Wang Sanmin , Li Fei , Li Linlin , Zou HaoRan , Liu Linlin 
Published: June 2022

Abstract
Herringbone gear transmission system is widely used in high-speed overloaded fields such as ships. Tooth deformation and installation error, which can cause meshing impact, load mutation, and load uneven, will seriously affect dynamic performance of the whole transmission system. Modification technology is the most effective way to achieve damping and noise reduction. In this article, we propose a three-dimensional (3D) modified tooth surface by grinding wheel along axial to the spiral rise and along radial to the parabolic movement based on the grinding principle and a multiobjective ant lion optimizer model for optimizing meshing performance and dynamic characteristics of herringbone gear transmission system and analyze the impact of optimized modifications of tooth profile, axial, and three dimension on meshing performance, loaded transmission error, load distribution coefficient and meshing impact, meshing stiffness, and vibration acceleration by the example. The results show that the 3D modification of optimization can not only eliminate the contact between the tooth side edge and tooth top edge but also eliminate the influence of installation error on contact performance. The root mean square values of the relative comprehensive vibration acceleration of tooth profile modification, axial modification, and 3D modification are reduced by 30.11%, 49.24%, and 61.41% compared with the standard, respectively. The 3D modification can greatly reduce tooth vibration, reduce resonance peak, and achieve the goal of noise reduction.
Comparing Complementary Kalman Filters Against SLAM for Collaborative Localization of Heterogeneous Multirobot Teams
Authors: Abruzzo Benjamin , Cappelleri David J. , Mordohai Philippos 
Published: June 2022

Abstract
This paper presents an investigation of collaborative localization for heterogeneous robots, resulting in a scheme for relative localization of a heterogeneous team of low-cost mobile robots. A novel complementary Kalman filter (CKF) approach is presented to address collaborative localization and mapping by optimally estimating the error states of the team. This indirect filter optimally combines the inertial/visual proprioceptive measurements of each vehicle with stereoscopic measurements made by unmanned ground vehicles (UGVs). An analysis is presented for both CKF and simultaneous localization and mapping (SLAM) approaches on maps containing randomly placed obstacles. In both simulation and experiments, we demonstrate the proposed methodology with a heterogeneous robot team. Six behavioral strategies, specifying the role and behavior of each robot, are simulated and evaluated for both CKF and SLAM approaches on maps containing randomly placed obstacles. Results show that the sources of error, image quantization, asynchronous sensors, and a nonstationary bias were sufficiently modeled to estimate the pose of the aerial robot. The results demonstrate localization accuracies of 2–4 cm, on average, while the robots operate at a distance from each other between 1 m and 4 m. The best performing behavior for the CKF approach maintained an average positional error of 2.2 cm and a relative error of 0.30% of the distance traveled for the entire team at the conclusion of maneuvers. For all multi-UGV strategies, the CKF approach outperformed the best SLAM results by a 6.7 cm mean error (0.48% of distance traveled).
Evaluation of a randomized nutrition intervention combining nutrition education and cooking workshops on dietary intakes and psychosocial determinants of performance in university football athletes
Authors: Larose David , Panahi Shirin , Jacob Raphaëlle , Drapeau Vicky 
Published: June 2022

Although nutrition influences performance, many athletes, including football players, do not meet nutritional requirements for their sport. The objective of this study was to determine the impact of a nutrition intervention combining nutrition education and cooking workshops on nutrition knowledge, intention, and perceived behavioral control in the preparation of healthy meals, dietary intakes, and diet quality in male university football players. Athletes ( n = 23; age: 22 ± 1 year) were randomly assigned to an intervention (2 h/week) ( n = 13) or control group ( n = 10) over three weeks. Questionnaires were completed before (pre), immediately after (post 1), and two months after the intervention (post 2). Dietary intakes and diet quality were evaluated from three web-based 24-h dietary recalls per visit. Mixed linear models for repeated measures with Tukey’s post hoc test were performed to determine the effects of the intervention on all outcomes. There was a group by time interaction for nutrition knowledge ( p = 0.002) that was higher immediately after and two months after the intervention compared to the control group. No differences on other outcomes were observed. Combining nutrition education and cooking workshops improved nutrition knowledge, but did not affect intention and perceived behavioral control in the preparation of healthy meals, dietary intakes, and diet quality in male university football athletes.
Cooperative Disengagement of Fas and Intercellular Adhesion Molecule-1 Function in Neoplastic Cells Confers Enhanced Colonization Efficiency
Authors: Liu Kebin , Caldwell Sheila A. , Abrams Scott I. 
Published: June 2022

AbstractUnderstanding the mechanisms of tumor progression is crucial toward the development of therapeutic interventions. Although the loss of sensitivity to cell death is a hallmark of neoplastic progression, it is likely one of several essential features that underlie a malignantly proficient or aggressive tumorigenic phenotype. Here, we identified intercellular adhesion molecule-1 (ICAM-1) as a molecule with expression coordinately regulated with Fas and inversely correlated with malignant phenotype between matched pairs of differentially aggressive malignant subpopulations in three mouse models. To determine whether coordinate expression of Fas and ICAM-1 regulated malignant behavior, tumor sublines were produced that expressed either lower levels of both Fas and ICAM-1, lower levels of Fas, or lower levels of ICAM-1 and then assessed for metastatic lung tumor growth. Tumor sublines rendered both Fas incompetent and ICAM-1 incompetent displayed significantly higher numbers of tumor nodules compared with tumor sublines separately expressing low levels of Fas or ICAM-1. However, all tumor sublines regardless of their Fas and ICAM-1 levels comparably infiltrated the lung, suggesting that Fas- and ICAM-1–based interactions ultimately influenced lung colonization efficiency. Overall, these data suggested that both Fas and ICAM-1 pathways cooperated to regulate tumor progression and that the coordinate down-regulation of Fas and ICAM-1 intensified malignant progression at the level of colonization. Thus, a FasloICAM-1lo phenotype may be characteristic of at least certain advancing, immune-resistant neoplastic subpopulations.
Up-Regulation of the Error-Prone DNA Polymerase κ Promotes Pleiotropic Genetic Alterations and Tumorigenesis
Authors: Bavoux Clarisse , Leopoldino Andréia Machado , Bergoglio Valérie , O-Wang Jiyang , Ogi Tomoo , Bieth Anne , Judde Jean-Gabriel , Pena Sérgio Danilo Junho , Poupon Marie-France , Helleday Thomas , Tagawa Masatoshi , Machado CarlosRenato , Hoffmann Jean-Sébastien , Cazaux Christophe 
Published: June 2022

Abstract
It is currently widely accepted that genetic instability is key to cancer development. Many types of cancers arise as a consequence of a gradual accumulation of nucleotide aberrations, each mutation conferring growth and/or survival advantage. Genetic instability could also proceed in sudden bursts leading to a more drastic upheaval of structure and organization of the genome. Genetic instability, as an operative force, will produce genetic variants and the greater the instability, the larger the number of variants. We report here that the overexpression of human DNA polymerase κ, an error-prone enzyme that is up-regulated in lung cancers, induces DNA breaks and stimulates DNA exchanges as well as aneuploidy. Probably as the result of so many perturbations, excess polymerase κ favors the proliferation of competent tumor cells as observed in immunodeficient mice. These data suggest that altered regulation of DNA metabolism might be related to cancer-associated genetic changes and phenotype.
Nitric Oxide Reverts the Resistance to Doxorubicin in Human Colon Cancer Cells by Inhibiting the Drug Efflux
Authors: Riganti Chiara , Miraglia Erica , Viarisio Daniele , Costamagna Costanzo , Pescarmona Gianpiero , Ghigo Dario , Bosia Amalia 
Published: June 2022

Abstract
Multidrug resistance (MDR) is a phenomenon by which cancer cells evade the cytotoxic effects of chemotherapeutic agents. It may occur through different mechanisms, but it often correlates with the overexpression of integral membrane transporters, such as P-glycoprotein (Pgp) and MDR-associated proteins (MRPs), with resulting decrease of drug accumulation and cellular death. Doxorubicin is a substrate of Pgp; it has been suggested that its ability to induce synthesis of nitric oxide (NO) could explain, at least in part, its cytotoxic effects. Culturing the human epithelial colon cell line HT29 in the presence of doxorubicin, we obtained a doxorubicin-resistant (HT29-dx) cell population: these cells accumulated less intracellular doxorubicin, were less sensitive to the cytotoxic effects of doxorubicin and cisplatin, overexpressed Pgp and MRP3, and exhibited a lower NO production (both under basal conditions and after doxorubicin stimulation). The resistance to doxorubicin could be reversed when HT29-dx cells were incubated with inducers of NO synthesis (cytokines mix, atorvastatin). Some NO donors increased the drug accumulation in HT29-dx cells in a guarosine-3′:5′-cyclic monophosphate–independent way; this effect was associated with a marked reduction of doxorubicin efflux rate in HT29 and HT29-dx cells, and tyrosine nitration in the MRP3 protein. Our results suggest that onset of MDR and impairment of NO synthesis are related; this finding could point to a new strategy to reverse doxorubicin resistance in human cancer.
Cdk Inhibition in Human Cells Compromises Chk1 Function and Activates a DNA Damage Response
Authors: Maude Shannon L. , Enders Greg H. 
Published: June 2022

Abstract
Cyclin-dependent kinases (Cdk) promote cell proliferation, are often deregulated in human cancers, and are targets of ongoing cancer chemotherapy trials. We show here that Cdk activity is also required in human cells to maintain function of the Chk1 pathway, a key component of the response to DNA damage or stalled replication. Chk1 expression was markedly reduced in primary fibroblasts and U2OS osteogenic sarcoma cells by treatment with small molecule Cdk inhibitors or induction of a dominant-negative mutant of Cdk2. The findings of decreased Chk1 activity and accumulation of Cdc25A, a protein targeted for degradation by Chk1, confirmed that Chk1 function was impaired. Furthermore, Cdk inhibition triggered a DNA damage response, characterized by the accumulation of activated forms of ATM and Chk2 as well as nuclear foci containing phosphorylated substrates of ATM/ATR, including histone H2AX (γH2AX). Time course experiments showed that the bulk of ATM activation followed Chk1 down-regulation. Chk1 RNA interference combined with partial inhibition of DNA replication was sufficient to evoke the DNA damage response. Conversely, ectopic expression of Chk1 blunted induction of γH2AX foci by Cdk inhibitors, indicating that Chk1 down-regulation was necessary to elicit the full phenotype. Finally, both Cdk and Chk1 inhibitors enhanced the cytotoxity of etoposide, a DNA-damaging agent. These results define a pathway through which Cdk inhibition can mediate DNA damage and potentially enhance the efficacy of extant cancer chemotherapies.
Vascularization of Melanoma by Mobilization and Remodeling of Preexisting Latent Vessels to Patency
Authors: Lu Weixin , Schroit Alan Jay 
Published: June 2022

AbstractTumors must manipulate the host vasculature to provide a blood supply adequate for their proliferation. Although tumors may arise as avascular masses, there is increasing evidence that some tumors begin to proliferate by first co-opting preexisting host blood vessels. By fluorescent vascular imaging, we provide evidence that the vasculature in orthotopically implanted melanoma arises from a preexisting red cell–deficient vascular network that remodels to patency to accommodate the requirements of the expanding tumor mass. Topical application of vascular endothelial growth factor to vascular beds generated immediate and robust vascular transitions that were morphologically similar to tumor-induced transitions. Nφ-nitro-l-arginine, a nitric oxide inhibitor, significantly inhibited the growth of a syngeneic K1735M2 melanoma by reducing blood supply to the tumor by a mechanism independent of endothelial cell proliferation. These findings suggest that tumor-induced remodeling of red cell–deficient vessels to patency contributes to tumor vascularization and growth.
Abstract 6287: Comparison of multiple myeloma provider for Black Americans and Caucasian Americans
Authors: Cheung Christian S. , Sweeney Nathan W. , Tiu Zena M. , Chmielewski Cynthia , Ahlstrom Jennifer M. 
Published: June 2022

Abstract
Background: Although Black Americans have 2-3 times multiple myeloma (MM) incidence and mortality as compared to Whites, increasingly research is demonstrating that many may have genetic characteristics that have better survival outcomes. Yet delayed diagnoses and inappropriate first lines of therapy often wipe out whatever “advantages” they may have had, keeping mortality statistics higher than would be expected (PMID: 25469920). For underserved populations, a number of commonly accepted disparities exist in myeloma, and more broadly, cancer care. Patients who receive treatment by a high-volume provider (myeloma specialist) is a factor that can help patients with MM to have a longer life expectancy (PMID: 31487686). Therefore, we analyzed whether there is a disparity between race and receiving care from a myeloma specialist which may contribute to the differences in survival that is being seen.
Methods: Validated real-world (RW) MM patient data was collected through HealthTree Cure Hub for Multiple Myeloma (healthtree.org). We examined the patient’s race which was either Caucasian American or Black American and whether or not they were being treated by a myeloma specialist. The association between race and specialist was examined by a chi-squared (X2) test.
Results: In this retrospective analysis of 2,871 MM patients, we compared Caucasian Americans (n= 2716) and Black Americans (n=155). Our analysis revealed a significant association between the two races and whether or not they had a myeloma specialist, X2(1, N = 2,871) = 5.85, p = 0.015. Black Americans were less likely to be treated by a myeloma specialist.
Conclusion: The findings of this RW analysis identify a glaring disparity in Black American MM patients and receiving care from a myeloma specialist. Addressing the underlying cause of the findings could extinguish other MM-related issues, such as delays in getting a correct diagnosis, lack of access to myeloma specialists, and many other non-medical issues leading to less-than-optimal outcomes from the majority of these Black American patients.
Citation Format: Christian S. Cheung, Nathan W. Sweeney, Zena M. Tiu, Cynthia Chmielewski, Jennifer M. Ahlstrom. Comparison of multiple myeloma provider for Black Americans and Caucasian Americans [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 6287.
Abstract 6297: New biomarker in liquid biopsies from breast and ovarian cancers
Authors: Ibrahim Safaa A. , Riehl Valerie , Beaman Kenneth D. 
Published: June 2022

Abstract
Breast and ovarian cancer (BOC) are among the top ten common cancers in females worldwide and remain important causes of death in women. There are shared risk factors between breast and ovarian cancer such as, BRCA1 and BRCA2. Detecting those risk factors are not enough to detect cancer early as approximately half of breast cancers develop in women who have no identifiable breast cancer risk factor other than gender (female) and age (over 40 years). Identification of specific biomarkers in liquid biopsies is required for early detection of BOC. BOC secretes exosomes in large quantities to blood circulation to communicate with different cell types such as immune cells to support cancer growth and metastasis. We detect a significant and readily measurable expression of a specific peptide from the a2 isoform vacuolar ATPase (a2NTD) in the exosomes isolated from the plasma collected from BOC patients. We found that a2NTD is highly expressed in stage III ovarian cancer. In this stage, the tumor spread outside of the abdomen and/or nearby lymph nodes. a2NTD positively correlates with the CA125 levels in plasma. These data show that plasma a2NTD correlates with tumor cell growth and metastasis. In addition, a2NTD associated breast cancer exosome can contribute to the pathophysiology of the disease as it induces PBMCs to secrete cancer promoting factors such as IL-8, CCL-2, and MMP-9. Importantly, those secreted factors promote angiogenesis and tumor cell migration. a2NTD promotes the immunosuppressive properties of neutrophils by stimulating arginase-1 release. Together, this data shows that a2NTD can be a potential diagnostic biomarker and therapeutic target in breast and ovarian cancer.
Citation Format: Safaa A. Ibrahim, Valerie Riehl, Kenneth D. Beaman. New biomarker in liquid biopsies from breast and ovarian cancers [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 6297.