bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementasi Sistem Irigasi Cerdas Berbasis IoT dan Machine Learning pada Pembibitan Pala di Papua Barat
Authors: Basri Hasan 
Published: August 2022

Kabupaten Fakfak merupakan daerah yang memiliki kekayaan alam dibidang pertanian dan nelayan, kondisi tanah yang subur memudahkan petani untuk mengolah tanahnya. Pada tahun 2018, hasil produksi pala sebesar 1.750 ton dengan luas lahan perkebunan pala di kabupaten fakfak sebesar 17.542 Ha yang tersebar di seluruh distrik kecuali distrik Karas, distrik Tomage dan distrik Bomberay. Jika ditinjau dari segi jumlah populasi pohon pala, jumlah tanaman pala di kabupaten fakfak tidak mengalami peningkatan signifikan setiap tahunnya. Hal ini desebabkan karena banyaknya tanaman pala yang mati saat dipindahkan ke kebun serta teknik pembibitan petani masih bersifat manual. Selain itu, juga dipengaruhi oleh kondisi cuaca yang tidak menentu sehingga mempengaruhi kelembapan tanah dan menghambat pertumbuhan tanaman tersebut. Saat musim kemarau, para petani biasanya menyiram tanaman bibit pala secara manual. Hal ini menjadi kendala petani saat mereka melakukan pembibitan sebanyak ribuan pohon. Mereka harus menyiram satu persatu pohon tersebut untuk memastikan bahwa kebutuhan pasokan air dan kelembaban tanah pada bibit tanaman pala tetap terjaga. Berangkat dari permasalahan tersebut, dibutuhkan sebuah metode yang mampu melakukan automasi sistem irigasi yang dapat menjaga kebutuhan nutrisi tanah dan mengurangi tingkat kematian pohon pala akibat cuaca. Penelitian ini berfokus pada perancangan sistem irigasi cerdas berbasis Internet Of Things (IoT) dengan mengkombinasikan beberapa sensor untuk mengukur suhu tanah dan suhu udara disekitar tanaman pala. Pemanfaatan teknologi machine learning juga diterapkan pada penelitian ini, teknologi machine learning digunakan untuk memprediksi kebutuhan irigasi pada lahan pertanian menggunakan berbagai parameter penginderaan yang diperoleh dari berbagai sensor. Seperti sensor kelembaban tanah dan suhu udara lingkungan sekitar tanaman. Semua data sensor tersebut akan dikumpulkan dan diolah menggunakan algoritma SVM dalam pengambilan keputusan secara otomatis dan real-time. penelitian ini mampu mengukur intensitas volume air dan kondisi lingkungan tanaman secara akurat yang berakibat pada peningkatan jumlah populasi pala di kabupaten fakfak.
Accurate Two-Phase Flow Simulation in Faulted Reservoirs by Combining Two-Point Flux Approximation and Mimetic Finite Difference Methods
Authors: Dong Rencheng , O. Alpak Faruk , F. Wheeler Mary 
Published: August 2022

Summary
Faulted reservoirs are commonly modeled by corner-point grids (CPGs). Because the two-point flux approximation (TPFA) method is not consistent on non-K-orthogonal grids, multi-phase flow simulation using TPFA on CPGs may have significant discretization errors if grids are not K-orthogonal. We developed a novel method to improve the simulation accuracy where the faults are modeled by polyhedral cells, and mimetic finite difference (MFD) methods are used to solve flow equations. We use a cut-cell approach to build the mesh for faulted reservoirs. A regular K-orthogonal grid is first constructed, and then cells are divided where fault planes are present. Most cells remain K-orthogonal while irregular non-K-orthogonal polyhedral cells can be formed with multiple cell divisions. We investigated three spatial discretization methods for solving the pressure equation on general polyhedral grids, including the TPFA, MFD, and TPFA-MFD hybrid methods. In the TPFA-MFD hybrid method, the MFD method is only applied to the part of the domain with severe grid non-K-orthogonality, while the TPFA method is applied to the rest of the domain.
We compare flux accuracy between TPFA and MFD methods by solving a single-phase flow problem. The reference solution is obtained on a rectangular grid, while the same problem is solved by TPFA and MFD methods on a grid with non-K-orthogonal cells near a fault. Fluxes computed using TPFA exhibit larger errors in the vicinity of the fault, while fluxes computed using MFD are still as accurate as the reference solution. We also compare saturation accuracy for two-phase (oil and water) flow in faulted reservoirs when the pressure equation is solved by different discretization methods. Compared with the reference saturation solution, saturation exhibits non-physical errors near the fault when the pressure equation is solved by the TPFA method. Because the MFD method yields accurate fluxes over general polyhedral grids, the resulting saturation solutions agree with reference saturation solutions with an enhanced accuracy when the pressure equation is solved by the MFD method. Based on the results of our simulation studies, we observe that the accuracy of the TPFA-MFD hybrid method is very close to the accuracy of the MFD method, while the TPFA-MFD hybrid method is computationally cheaper than the MFD method.
Maridesulfovibrio caeruleilacunae sp. nov. and Maridesulfovibrio oucae sp. nov . , two sulfate-reducing bacteria isolated from Yongle Blue Hole of the South China Sea
Authors: Dai Wei , Sun Wen , Fu Tianyu , Jia Chao , Fu Liang , Yao Peng , Yang Zuosheng , Zhou Shun , Shi Xiaochong , Zhang Xiao-Hua 
Published: August 2022

Abstract
A reconstructed phylogenetic tree indicated that the novel strain JC010T and JC022T both formed a lineage with strains in the same genus Maridesulfovibrio. The novel strains designated JC010T and JC022T were both isolated from seawater of the Yongle Blue Hole, South China Sea. The two strains were both Gram-staining-negative, obligately anaerobic, motile, arcuation, and flagellated marine bacteria. Based on the 16S rRNA gene sequence analysis, sequence similarities of strain JC010T and JC022T were less than 97.9% and 97.3% against all validly named species in the genus Maridesulfovibrio respectively and there is a sequence similarity of 98.4% between strain JC010T and JC022T. The temperature, pH and NaCl range for growth of strain JC010T was 4–37°C (optimum 28°C), 4.0–9.0 (optimum 7.0) and 1.0-5.5% (w/v; optimum 4.0%). As for strain JC022T, growth occurred at the temperature range of 10–37°C (optimum 28°C) and pH range of 4.0–8.0 (optimum 7.0) in the presence of 2.5-6.0% (w/v) NaCl (optimum 3.0%). The predominant isoprenoid quinone of strain JC010T and JC022T were both identified as menaquinone-7 (MK-7). The dominant fatty acids (> 10%) of strain JC010T and JC022T were summed features 9 (iso-C17:1 ω9c), C16:0, iso-C15:0, summed features 3 (C16:1 ω6c/ C16:1 ω7c). The major polar lipids of strain JC010T and JC022T both included one phosphatidylcholine (PC), one phosphatidylethanolamine (PE), one phosphatidylglycerol (PG), and two phospholipids (PL1-2). For strain JC022T, it also included other three unidentified lipids (L1-3). The genomic DNA G + C content was 49.8 mol % and 48.0 mol % for strain JC010T and JC022T. Based on physiological and biochemical characteristics and whole-genome comparisons, we propose two novel species, Maridesulfovibrio caeruleilacunae sp. nov. and Maridesulfovibrio oucae sp.nov within the same genus Maridesulfovibrio. The type strain of Maridesulfovibrio caeruleilacunae and Maridesulfovibrio oucae are JC010T (= JCM 39061T = MCCC 1K03847T) and JC022T (= JCM 39062T = MCCC 1K03848T) respectively.
Genetic analysis of resistance to powdery mildew on 7Mg chromosome of Wheat–Aegilops geniculata,development and utilization of specific molecular markers
Authors: Wang Yongfu , Fan Jianzhong , Xiao Yi , Feng Xianbo , Zhang Hong , Chen Chunhuan , Ji Wanquan , Wang Yajuan 
Published: August 2022

Abstract
Background: Blumeria graminis f. sp. Tritici (Bgt) is prevalent in the main wheat-producing regions of China and result in serious yield losses in recent years. Breeding resistant cultivars is the most environmentally sound measure of disease control. Aegilops geniculata Roth, a close relative of common wheat, is an important and valuable disease resistance gene donor for wheat improvement.Results: In this study, to validate powdery mildew resistance on chromosome 7Mg, two genetic populations were constructed and analyzed. Wheat – Ae. geniculata 7Mg disomic addition line and 7Mg (7A) alien disomic substitution line crossed with susceptible Yuanfeng175 of susceptible powdery mildew respectively to form generations F1 and F2. Cytological examination, in situ hybridization (ISH), and functional molecular markers analysis showed that alien chromosomes could be inherited stably, produce different gamete types and enrich the intermediate materials for wheat genetic variation. The populations were inoculated with the physiological race E09 of powdery mildew at seedling stage. The results revealed that the plants showed high resistance to powdery mildew with chromosome 7Mg. Besides, more specific markers were developed to verify chromosome 7Mg resistance based on SLAF-seq technique. Then, 84 specific molecular markers were obtained about chromosome 7Mg. Among them, four markers were selected randomly to checked in two genetic populations. In summary, the above analysis confirmed that a dominant high powdery mildew resistance gene inherited were located on the chromosome 7Mg of Aegilops geniculate. Conclusions: The results provide a basis for resistance gene mapping and specific marker development in future.
Serum type I collagen carboxyl-terminal peptide β glypeptide was positively associated with osteoporosis and skeletal muscle loss
Authors: chen lingyan , wu jiayu , ren weiying , li xi , luo man , hu yu 
Published: August 2022

Abstract
Background
Previous studies have presented osteoporosis and sarcopenia frequently occur concomitantly in elderly. Type I collagen carboxyl-terminal peptide β (β-CTX) usually increases in osteoporosis. However, the relationship between β-CTX and muscle mass remains unclear. This study aimed to explore the association between serum β-CTX and the risk of sarcopenia in elderly inpatients.
Methods
228 patients > 65 years were recruited in this cross-sectional study. Dual-energy X-ray scanning (DXA) was used to access skeletal muscle and bone mass. Skeletal muscle mass index (SMI) was defined as appendicular skeletal muscle mass (ASM) divided by height2. Serum markers of bone remodeling were detected by electrochemical immunofluorescence assay.
Results
Serum concentration of β-CTX as well as the prevalence of osteoporosis were higher in low SMI group than in the normal SMI group. Correlation analysis showed that serum β-CTX levels negatively correlated with SMI and bone mass. Across the quartiles of serum β-CTX, total skeletal muscle mass (TSM), ASM, SMI, total bone mass (TBM) and bone mass at various sites including the limbs, spine and pelvis decreased significantly, wherea the prevalence of low SMI and osteoporosis increased. Logistic regression analysis showed the higher quartiles of serum β-CTX had an increased risk of low SMI and osteoporosis, compared to the lowest quartile (the third and fourth quartiles for low SMI and the fourth quartile for osteoporosis).
Conclusions
With increasing serum β-CTX levels, muscle and bone mass decreased in Chinese elderly inpatients. Serum β-CTX was positively associated with the risk of osteoporosis and muscle loss.
Glioblastoma Multiforme Imitating a Cavernoma: A Unique Image Case Report
Authors: Qazi Saba Saleem , Baqai Muhammad Waqas Saeed , Faisal Kunwer Sufyan , Shah Syed Muhammad Ismail , Aziz Hafiza Fatima 
Published: August 2022

Abstract
Glioblastomas multiforme (GBM) is the most common primary malignant brain tumor. We highlight an unusual case of a 54-year-old woman, neurologically intact with a diagnostically challenging lesion. The patient's Magnetic Resonance Imaging (MRI) revealed a left frontal lesion with surrounding edema and a hemosiderin ring misleading it to be a cavernoma. Intra-operatively, the lesion was found to be a solid tumor with hematoma around and was confirmed to be (GBM) on histopathology. In conclusion, the dilemma associated with our patient’s radiological findings and a longstanding history of epilepsy is rare and a diagnostic challenge
Application of modified gemcitabine-loaded solid lipid nanoparticle in the treatment of pancreatic cancer patient-derived xenograft model
Authors: Inkoom Andriana , Ndemazie Nkafu , Smith Taylor , Frimpong Esther , Bulusu Raviteja , Poku Rosemary , Zhu Xue , Han Bo , Trevino Jose , Agyare Edward 
Published: August 2022

Abstract
Purpose: Gemcitabine (Gem) remains a preferred first-line anticancer drug used for the treatment of pancreatic cancer (PCa). However, rapid metabolism and systemic instability (short half-life) have limited its therapeutic efficacy. The purpose of this study was to modify Gem to a more stable form, 4-(N)-stearoyl-gemcitabine (4NSG), and to evaluate its efficacy in patient-derived xenograft (PDX) mouse models harboring African American (AA) and Caucasian (White) patients' tumors. Methods: 4NSG was developed and characterized using high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and elemental analysis. 4NSG-loaded solid lipid nanoparticles (4NSG-SLN) were developed using the cold homogenization technique and characterized. Cytotoxicity, cell migration, and clonogenic studies were performed to determine the effectiveness of 4NSG-SLN against AA primary PCa cells (PPCL-192, PPCL-135) and White PCa primary cells (PPCL-46, PPCL-68). Pharmacokinetics (PK), and tumor efficacy studies were conducted using PDX mouse models bearing tumors from AA and white PCa patients. Results: The effective particle size of 4NSG-SLN was 82 nm and (IC50) values of 4NSG-SLN treated AA cells (PPCL-192, 9 ± 1.1 µM and PPCL-135, 11 ± 1.3 µM) and White cells (PPCL-46, 12 ± 2.1 and PPCL-68, 22 ± 2.6) were found to be significantly lower compared to Gem treated AA cells (PPCL-192, 57 ± 1.5 µM and PPCL-135, 56 ± 1.5 µM) and White cells (PPCL-46, 56 ± 1.8 µM and PPCL-68, 57 ± 2.4 µM). The area under the curve (AUC), half-life, and clearance pharmacokinetic parameters for 4NSG-SLN were 3-4-fold higher compared to that of GemHCl. 4NSG-SLN treated PDX mice exhibited a two-fold decrease in tumor growth inhibition in PDX mice bearing AA and Whites patients' tumors compared to Gem treated PDX mice bearing AA and Whites tumors. Conclusion: 4NSG-SLN significantly improved the pharmacokinetics of Gem, enhanced systemic stability of Gem, and increased its antitumor efficacy in PCa PDX mice bearing AA and White tumors.
Acceptability and expected changes in parental practices after an online behavioural parent program for ADHD during the Covid-19 pandemic
Authors: Rahali Meryem , Fongaro Erica , Franc Nathalie , Nesensohn Jessica , Purper-Ouakil Diane , Kerbage Hala 
Published: August 2022

Abstract
Background
Attention deficit hyperactivity disorder (ADHD) is a common neurodevelopmental disorder in children and adolescents, characterized by attention deficit, hyperactivity, and impulsivity. Treatments for this population should be multidisciplinary and must be initiated as soon as possible. Non-pharmacological interventions for ADHD include psychoeducation, parent behavioural training programs and school interventions and accommodations. In response to the COVID-19 pandemic, we developed an online version of a combined psychoeducation and behavioural training program to facilitate access to mental health treatment and ensure continuity of care. The study assessed the acceptability of this online parent training program, among parents and/or caretakers of children and adolescents with ADHD.
Methods
The program consisted of 10 online sessions over the course of 2 consecutive days (5 sessions a day). Satisfaction, usefulness and general comments about the program were assessed with open-ended questions and visual-analogous scales. Parents/caretakers’ use of strategies to manage behavioural problems was assessed using the Parenting and Family Adjustment Scales.
Results
A total of 206 parents participated in the online program and 175 of them completed the evaluation. Overall, participants were satisfied with the content of the program and found it beneficial for their child. The engagement was high and no major obstacles were identified to participation, except some internet connection issues. More than half of participants had already used some of the strategies included in the program. Despite this, some difficulties in implementing new strategies were observed.
Conclusions
Our study contributes to the growing body of papers evaluating online behavioral training program to improve their efficacy and content. With these measures, we hope to improve engagement in online psychoeducation and behavioural therapy programs. Future research evaluating online behavioural training programs should focus on ways to make them more accessible and adaptable to families’ obstacles.
Novel Domain Knowledge Encoding Enables Machine Learning of Rapid, Expert-level Segmentation of Cardiac Computed Tomography
Authors: Feng Ruibin , Deb Brototo , Ganesan Prasanth , Tjong Fleur , Rogers Albert , Ruipérez-Campillo Samuel , Somani Sulaiman , Rodrigo Miguel , Clopton Paul , Zou James , Zaharia Matei , Narayan Sanjiv 
Published: August 2022

Abstract
Ablation is a common therapeutic procedure for atrial fibrillation (AF), that is guided to specific targets in the heart often after laborious segmentation of computed tomography. Machine learning (ML) can automate such tasks but requires large training datasets. Inspired by natural intelligence, which builds conceptual models to learn without large datasets, we mathematically encoded domain knowledge of atrial geometry to accelerate ML segmentation. In test cohorts (N=160) at 2 institutions, Dice scores were 96.7% (IQR: 95.3% to 97.7%) and 93.5% (IQR: 91.9% to 94.7%) with similar anatomic agreement to experts (r=0.99; p<0.0001). In a prospective study of patients undergoing AF ablation (N=42), our approach reduced segmentation time by 85% (2.28±0.8 vs 15.0±6.9 minutes; p<0.0001), with similar Dice (p=0.07) versus experts. This approach may broaden the availability of AF ablation, and more broadly shows that encoding of domain knowledge may reduce the dependence of ML on large training datasets.
Effects of protein levels in commercial diets on growth performance and survival rate of rabbitfish (Siganus guttatus) at the nursing stage
Authors: Linh Nguyen Quang , Phuong Tran Vinh , Tuan Nguyen Anh , Tram Nguyen Duy Quynh , Thuan Nguyen Duy 
Published: August 2022

Abstract
This study aims to determine how the levels of protein in commercial diets affect growth and survival rates of Siganus guttatus from the fry to fingerling stage. The experiment allocated 3 levels of protein in the diets: 35% (trial 1); 40% (trial 2) and 45% (trial 3) and each trial was repeated three times. The 30-day-old fry in this study had an initial length and weight of 18.25 ± 0.15 mm fish -1 and 0.036 ± 0.50 g fish -1 , respectively. In experimental setup, the stocking density was set to 1,000 fish m -3, and all the experimental fish were arranged in a composite tank with a volume of 1.0 m 3 . After 30 days of rearing, the weight of fingerlings in formula trial 1 reached 1.52 ± 0.01 g fish -1 , which was lower than that of trial 2, where fingerling weight was reported to be 1.69 ± 0.01 g fish -1 but was showed no difference from trial 3, which obtained a weight of 1.58 ± 0.08 g fish -1 . The length of fingerlings in trial 1 reached 36.92 ± 0.60 mm fish -1 , which was lower than that in trial 3 (45% CP) with lengths of 38.89 ± 0.43 mm fish -1, but not significantly different in trial 2 (40% CP) with a length of 37.97 ± 0.35 mm fish -1 . The survival rate of fingerlings ranged from 67.27 to 72.33%. Meanwhile, the herd distribution coefficient variation (CVw) in the treatment using 45% protein (trial 3) was the highest at 7.42% (p < 0.05).
A Drug Delivery System Constructed by a Fusion Peptide Capturing Exosomes Targets to Titanium Implants Accurately Resulting the Enhancement of Osseointegration Peri-implant
Authors: Li Xuewen , Ma Shiqing , Xu Shendan , Ma Xinying , Zhao Zhezhe , Hu Han , Deng Jiayin , Peng Cheng , Liu Zihao , Wang Yonglan 
Published: August 2022

Abstract
Background
Exosomes derived from bone marrow mesenchymal stem cells (BMSC-exos) have been shown triggering osteogenic differentiation and mineralization of MSCs,but exosomes administered via bolus injections are rapidly sequestered and cleared. Therefore, we considered the implant as a new organ of patient's body and expected to find a method to treat implant with BMSC-exos in vivo directly.
Methods
A fusion peptide (PEP), as a drug delivery system (DDS) which contained a titanium-binding peptide (TBP) possessing the ability to selectively bind to the titanium surface and another peptide CP05 being able to capture exosomes expertly, is constructed to modify the titanium surface.
Results
Both in vitro and in vivo experiments prove PEP retains the ability to bind titanium and exosome simultaneously, and the DDS gain the ability to target exosomes to titanium implants surface following enhancing osseointegration post-implantation. Moreover, the DDS constructed by exosomes of diverse origins shows the similar combination rate and efficiency of therapy.
Conclusion
This drug delivery system demonstrates the concept that EXO-PEP system can offer an accurate and efficient therapy for treating implants with long-term effect.
Electroconductive PEDOT Nanoparticle Integrated Scaffolds for Spinal Cord Tissue Repair
Authors: Serafin Aleksandra , Rubio Mario Culebras , Carsi Marta , Ortiz-Serna Pilar , Sanchis Maria J. , Garg Atul K. , Oliveira J. Miguel , Koffler Jacob , Collins Maurice N 
Published: August 2022

Abstract
Background
Hostile environment around the lesion site following spinal cord injury (SCI) prevents the re-establishment of neuronal tracks, thus significantly limiting the regenerative capability. Electroconductive scaffolds are emerging as a promising option for SCI repair, though currently available conductive polymers such as polymer poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) present poor biofunctionality and biocompatibility, thus limiting their effective use in SCI tissue engineering (TE) treatment strategies.
Methods
PEDOT NPs were synthesized via chemical oxidation polymerization in miniemulsion. The conductive PEDOT NPs were incorporated with gelatin and hyaluronic acid (HA) to create gel:HA:PEDOT-NPs. Morphological analysis of both PEDOT NPs and scaffolds was conducted via SEM. Further characterisation included dielectric constant and permittivity variances mapped against morphological changes after crosslinking, Young’s modulus, FTIR, DLS, swelling studies, rheology, in-vitro, and in-vivo biocompatibility studies were also conducted.
Results
Incorporation of PEDOT NPs increased the conductivity to 8.3×10− 4±8.1×10− 5 S/cm. The compressive modulus of the scaffold was tailored to match the native spinal cord at 1.2 ± 0.2 MPa, along with controlled porosity. Rheological studies of the hydrogel showed excellent 3D shear-thinning printing capabilities and shape fidelity post-printing. In-vitro studies showed the scaffolds are cytocompatible and an in-vivo assessment in a rat SCI lesion model shows glial fibrillary acidic protein (GFAP) upregulation not directly in contact with the lesion/implantation site, with diminished astrocyte reactivity. Decreased levels of macrophage and microglia reactivity at the implant site is also observed. This positively influences the re-establishment of signals and initiation of healing mechanisms. Observation of axon migration towards the scaffold can be attributed to immunomodulatory properties of HA in the scaffold caused by a controlled inflammatory response. HA limits astrocyte activation through its CD44 receptors and therefore limits scar formation. This allows for a superior axonal migration and growth towards the targeted implantation site through the provision of a stimulating microenvironment for regeneration.
Conclusions
Based on these results, the incorporation of PEDOT NPs into Gel:HA biomaterial scaffolds enhances not only the conductive capabilities of the material, but also the provision of a healing environment around lesions in SCI. Hence, gel:HA:PEDOT-NPs scaffolds are a promising TE option for stimulating regeneration for SCI.