bluuebunny/crossref_metadata_2025_split huggingface dataset:
AAMP is a binding partner of costimulatory human B7-H3
Authors: Ciprut Sara , Berberich Anne , Knoll Maximilian , Pusch Stefan , Hoffmann Dirk , Furkel Jennifer , Ward Gahlawat Aoife , Kahlert-Konzelamnn Lena , Sahm Felix , Warnken Uwe , Winter Martin , Schnölzer Martina , Pusch Sonja , von Deimling Andreas , Abdollahi Amir , Wick Wolfgang , Lemke Dieter 
Published: June 2022

Abstract

Background
Targeted immunotherapies are of growing interest in the treatment of various cancers. B7 homolog 3 protein (B7-H3), a member of the co-stimulatory/-inhibitory B7-family, exerts immunosuppressive and pro-tumorigenic functions in various cancer types and is under evaluation in ongoing clinical trials. Unfortunately, interaction partner(s) remain unknown which restricts the druggability.

Methods
Aiming to identify potential binding partner(s) of B7-H3, a yeast two-hybrid and a mass spectrometry screen were performed. Potential candidates were evaluated by bimolecular fluorescence complementation (BiFC) assay, co-immunoprecipitation (co-IP), and functionally in a 3H-thymidine proliferation assay of Jurkat cells, a T-cell lineage cell line. Prognostic value of angio-associated migratory cell protein (AAMP) and B7-H3 expression was evaluated in isocitrate dehydrogenase 1 wildtype (IDH1wt) glioblastoma (GBM) patients from The Cancer Genome Atlas (TCGA)-GBM cohort.

Results
Of the screening candidates, CD164, AAMP, PTPRA, and SLAMF7 could be substantiated via BiFC. AAMP binding could be further confirmed via co-IP and on a functional level. AAMP was ubiquitously expressed in glioma cells, immune cells, and glioma tissue, but did not correlate with glioma grade. Finally, an interaction between AAMP and B7-H3 could be observed on expression level, hinting toward a combined synergistic effect.

Conclusions
AAMP was identified as a novel interaction partner of B7-H3, opening new possibilities to create a targeted therapy against the pro-tumorigenic costimulatory protein B7-H3.
Materials, electrodes and electrolytes advances for next-generation lithium-based anode-free batteries
Authors: Pal Shubhadeep , Zhang Xiaozhe , Babu Binson , Lin Xiaodong , Wang Jiande , Vlad Alexandru 
Published: June 2022

Abstract
The high volumetric stack energy density (∼750 Wh L−1) is a must for grasping the practical application of electric vehicles with more than 100 km per day driving range. Such achievement requires significant advances in state-of-the-art battery technologies. The anode-free, derived from the metal-battery concept, germinates as one of the future potential battery configurations due to the highest, nearly theoretical gravimetric and volumetric energy density. Thus, moving from the graphite-based anode, where lithium is stored as ions, to anode-free cells, wherein lithium is plated as metal, can change the scenario of the electrochemical energy storing devices both in terms of energy density and fundamental mechanism. Although an anode-free battery theoretically provides higher stack energy density than a Li-ion battery, current developments are still underoptimized as these can barely hold for several cycles at room temperature due to the absence of an active lithium reservoir and still severe losses at the anode side. Hence, electrolyte engineering with suitable electrode material choice is highly desirable and extremely challenging in realizing next-generation anode-free batteries. Herein, we summarize the current developments and achievements in the direction of anode-free batteries. Central emphasis is set on electrolyte chemistries that should on one hand allow for high-efficiency initial nucleation, followed by subsequent electrodeposition and electrodissolution of lithium metal, while also forming stable anodic interphases with the high energy cathode materials currently in use. We also prospect for better batteries with higher energy density beyond the present status.
Cash-like vouchers improve psychological well-being of vulnerable and displaced persons fleeing armed conflict
Authors: Quattrochi John , Bisimwa Ghislain , van der Windt Peter , Voors Maarten 
Published: June 2022

AbstractThe psychological burden of conflict-induced displacement is severe. Currently, there are 80 million displaced persons around the world, and their number is expected to increase in upcoming decades. Yet, few studies have systematically assessed the effectiveness of programs that assist displaced persons, especially in settings of extreme vulnerability. We focus on eastern Democratic Republic of Congo, where myriad local armed conflicts have driven cycles of displacement for over 20 years. We conducted a within-village randomized field experiment with 976 households, across 25 villages, as part of the United Nations’ Rapid Response to Population Movements program. The program provided humanitarian relief to over a million people each year, including vouchers for essential nonfood items, such as pots, pans, cloth, and mattresses. The vouchers led to large improvements in psychological well-being: a 0.32 standard deviation unit (SDU) improvement at 6 weeks, and a 0.18 SDU improvement at 1 year. There is no evidence that the program undermined social cohesion within the village, which alleviates worries related to programs that target some community members but not others. Finally, there was no improvement in child health.
Sharing Burdensome Work
Authors: Kandiyali Jan 
Published: June 2022

Abstract
In this paper, I defend the proposal that certain forms of work—specifically forms that are socially necessary but involve the imposition of considerable burdens—be shared between citizens. I argue that sharing burdensome work would achieve several goals, including a more equal distribution of the benefits and burdens of work, a greater appreciation of each other's labour contributions, and an amelioration of problematic inequalities of status. I conclude by considering three objections: that sharing burdensome work would (1) involve morally unacceptable constraints on freedom, (2) be prohibitively inefficient, and (3) forbid mutually advantageous trades. I argue that none of these objections succeed.
Screening Mungbean Accessions for Susceptibility to Soybean Fungal Diseases in Iowa
Authors: Batzer Jean C. , Singh Arti , Platner Evelyn , Kandel Yuba , Mueller Daren S. 
Published: June 2022

United States’ sales of mungbean ( Vigna radiata L. Wilczek) products and other plant-based protein sources exceeded $1 billion in 2020. Most mungbean consumed in the United States is imported, but it has considerable potential as a domestic crop. Its heat and drought tolerance give U.S. farmers an alternative to soybean and corn crop rotation. Mungbean is a 60- to 90-day crop with similar equipment needs, chemical inputs, and storage requirements to those of soybean and has a developed market. Its vulnerability to diseases in the United States is unknown. Thus, we compared the susceptibility of 10 mungbean genotypes to 8 soybean diseases in the lab. We also monitored disease occurrence in sentinel field plots in Iowa. Mungbean accessions showed a wide range of susceptibility to Cercospora kikuchii, Colletotrichum gloeosporioides, Diaporthe caulivora, Fusarium oxysporum, F. virguliforme, Macrophomina phaseolina, Pythium sylvaticum, and Sclerotinia sclerotiorum. Symptoms of frogeye leaf spot, sudden death syndrome, and Rhizoctonia root and crown rot, common soybean diseases, were observed on mungbean in the field; isolates were identified as Cercospora sojina, F. virguliforme, and Rhizoctonia solani, and Koch's postulates were conducted. C. sojina and F. virguliforme are newly reported as causing disease in field-grown mungbean.
A case report: subacute combined degeneration of the spinal cord and pernicious anemia caused by autoimmune gastritis
Authors: Sun Zhihuan , Yu Xiaofei 
Published: June 2022

Introduction:
Autoimmune gastritis causing both subacute combined degeneration of the spinal cord and pernicious anemia is rare in clinical practice. Here, we report a case of subacute combined degeneration of the spinal cord and pernicious anemia resulting from vitamin B12 deficiency due to autoimmune gastritis.

Patient concerns:
A 66-year-old woman presented with a 2-month history of numbness in her extremities.

Diagnoses:
The diagnoses were (1) autoimmune gastritis (2) subacute combined degeneration of the spinal cord (3) pernicious anemia (4) hypergastrinemia (5) chronic lymphocytic thyroiditis.

Interventions:
The patient received intramuscular methylcobalamin treatment for 5 days, followed by oral methylcobalamin daily.
Outcomes: Symptoms improved, and anemia recovered in the second month after discharge. She discontinued her medication afterward, and the neurological symptoms recurred.

Conclusions:
Autoimmune gastritis can lead to several diseases if not intervened in the early course. Neuropathy and hematopathy recur with treatment discontinuity. Methylcobalamin and adenosylcobalamin are unlikely to be more effective than vitamin B12.
I-131 false-positive uptake in a thymic cyst with expression of the sodium-iodide symporter: A case report
Authors: Yano Erina , Nakajo Masatoyo , Jinguji Megumi , Tani Atsushi , Kitazono Ikumi , Yoshiura Takashi 
Published: June 2022

Rationale:
I-131 radioiodine false-positive findings in postoperative patients with differentiated thyroid cancer (DTC) should be recognized to avoid unnecessary therapies.

Patient concerns and diagnoses:
A 50-year-old man underwent I-131 therapy 3 times, including the initial ablative therapy after total thyroidectomy for papillary thyroid cancer. The initial I-131 posttherapeutic whole-body scintigraphy showed 2 cervical and one superior mediastinal focal I-131 positive uptake lesions. The serum thyroglobulin level was negative every time when the radioiodine therapy was performed. Although the 2 cervical positive uptake lesions disappeared after the second therapy, the superior mediastinal I-131 positive uptake persisted even after the third therapy, and this lesion was suspicion of I-131 therapy-resistant node metastasis.

Interventions and outcomes:
The lesion was resected, and the pathological diagnosis with immune-histochemical analysis was a thymic cyst with thymic epithelial cells having a weak expression of the sodium-iodide symporter (NIS).

Lessons:
The false-positive result may be attributed to the NIS expression in the thymic cyst epithelial cells. It is necessary to include a thymic cyst in the differential diagnosis, when I-131 uptake is noted in the superior mediastinal region on I-131 posttherapeutic scans of patients with postoperative DTC. Although the I-131 positive uptake in a thymic cyst may be influenced by the I-131 administered dose and scan timing after I-131 administration, the NIS expression may be essential to the false-positive uptake in a thymic cyst.
How physical techniques improve the transdermal permeation of therapeutics: A review
Authors: Gao Yan , Du Lina , Li Qian , Li Qi , Zhu Lin , Yang Meiyan , Wang Xiu , Zhao Bonian , Ma Shan 
Published: June 2022

Background:
Transdermal delivery is very important in pharmaceutics. However, the barrier function of the stratum corneum hinders drugs absorption. How to improve transdermal delivery efficiency is a hot topic. The key advantages of physical technologies are their wide application for the delivery of previously nonappropriate transdermal drugs, such as proteins, peptides, and hydrophilic drugs. Based on the improved permeation of drugs delivered via multiple physical techniques, many more diseases may be treated, and transdermal vaccinations become possible. However, their wider application depends on the related convenient and portable devices. Combined products comprising medicine and devices represent future commercial directions of artificial intelligence and 3D printing.

Methods:
A comprehensive search about transdermal delivery assisted by physical techniques has been carried out on Web of Science, EMBASE database, PubMed, Wanfang Database, China National Knowledge Infrastructure, and Cochrane Library. The search identified and retrieved the study describing multiple physical technologies to promote transdermal penetration.

Results:
Physical technologies, including microneedles, lasers, iontophoresis, sonophoresis, electroporation, magnetophoresis, and microwaves, are summarized and compared. The characteristics, mechanism, advantages and disadvantages of physical techniques are clarified. The individual or combined applicable examples of physical techniques to improve transdermal delivery are summarized.

Conclusion:
This review will provide more useful guidance for efficient transdermal delivery. More therapeutic agents by transdermal routes become possible with the assistance of various physical techniques.
Plasma neurofilament light chain: A biomarker predicting severity in patients with acute ischemic stroke
Authors: Wu Jixia , Wu Daqing , Liang Youbao , Zhang Zhen , Zhuang Lei , Wang Zhaoping 
Published: June 2022

Neurofilament light chain (NfL) levels have proved to be a good biomarker in cerebrospinal fluid (CSF) correlating with the degree of neuronal injury and neurodegeneration. However, little is known about the value of plasma neurofilament light chain (pNfL) levels in predicting the clinical prognosis of patients with acute cerebral infarction. This study aimed to explore whether pNfL could be used as a biomarker to predict the severity of the outcomes of acute ischemic stroke (AIS).
Patients with AIS were included from the Department of Neurology of the First People’s Hospital of Bengbu City from January 2018 to May 2019, as well as health control (HC). The plasma levels of NfL in patients with AIS (n = 60) at 2 days, 7 days, and 6 months after stroke, as well as in HCs (n = 60) were measured by electrochemiluminescence immunoassay(ECL) on the Meso Scale Discovery platform. Stroke severity was analyzed at admission using the National Institutes of Health Stroke Scale score. Functional outcomes were assessed at different times using the modified Rankin Scale (mRS) and Barthel Index.
The mean level of pNfL in patients with ischemic stroke (IS) at 2 days (225.86 pg/L) after stroke was significantly higher than that in HC (107.02 pg/L) and gradually increased 7 days after stroke (316.23 pg/L) (P < .0001). The mean level of pNfL in patients with IS at 6 months after stroke was 173.38 pg/L, which was still significantly higher than that of HC. The levels of pNfL at 7 days after stroke independently predicted modified Rankin Scale scores (mRS) (R = 0.621, P < .001), Barthel Index (R = –0.716, P < .001), and National Institutes of Health Stroke Scale (R = –0.736, P < .001). The diagnostic severity and prognosis were evaluated by ROC curve, an area under the receiver operator curve of 0.812 (P = .001, 95% CI: 0.69–0.93) at 7 days.
Plasma NfL levels reflect neuronal injury after AIS. It changes with time and has a certain relationship with prognosis and may be a promising biomarker for predicting the severity of neuroaxonal injury in patients with acute IS.
Ultrasound-guided dorsal penile nerve block is a safe block in hypospadias surgery: A retrospective clinical study
Authors: Yiğit Doğakan , Özen Volkan , Kandirici Aliye , Dokucu Ali İhsan 
Published: June 2022

Caudal and dorsal penile nerve blocks are commonly used regional anesthesia methods in hypospadias surgery. Some studies have reported that regional anesthesia methods are risk factor for the development of postoperative complications following hypospadias surgery. The aim of the current study is to evaluate the relationship between postoperative complications and regional anesthesia methods used in distal hypospadias surgery.
Forty-nine distal hypospadias patients were included. Patients had either received caudal or ultrasound (US)-guided dorsal penile nerve block. The age, type of hypospadias, regional anesthesia method, operation time, and postoperative complications were recorded. Fisher exact test and Mann–Whitney U tests were used to compare the data.
Caudal epidural block was used in 25 (51%) patients and US-guided dorsal penile nerve block in 24 (49%) patients. There was no statistically significant difference between the groups regarding the types of hypospadias, operation time, and age. Fistula developed in 4 (16%) patients in the caudal block group and in none of the patients in the dorsal penile nerve block group. Fistula rates were statistically significantly different between the groups (P = .030).
Conflicting data are found in the literature on the long-term postoperative complications of the regional anesthesia techniques used in hypospadias surgery. In our study, all patients with urethrocutaneous fistula were in the caudal block group. We believe that our study will contribute to the literature as it is the only study comparing caudal block with US-guided dorsal penile nerve block using in-plane technique in terms of postoperative complications in hypospadias surgery.
Decorin inhibits proliferation and metastasis in human bladder cancer cells by upregulating P21
Authors: Chen Hongjie , Wang Ziyi , Yang Ninggang , Zhang Jun , Liang Zhong 
Published: June 2022

Migration of bladder cancer (BC) cells poses a substantial threat to human health. It is critical to elucidate the mechanism of BC invasion and progression for surgical treatment and the prognosis of patients. Decorin is of interest as an anticancer treatment that can play a vital role in regulating tumorigenesis.
The effect of decorin expression on survival in clinical patients was screened and analyzed using bladder urothelial carcinoma data from the Cancer Genome Atlas (TCGA) database. The differential expression of transforming growth factor-β1 (TGF-β1) in tumors was compared against that of normal samples to analyze the correlation between them. MTT, flow cytometry, and Wound/Transwell assays were used to detect cell proliferation, cycle arrest, apoptosis, migration, and invasion.
Analysis of TCGA data showed that decorin expression was significantly lower in bladder urothelial carcinoma samples than in normal tissues, while TGF-β1 expression did not change significantly. We found that decorin was correlated with TGF-β1 expression in bladder urothelial cancer. In addition, decorin blocked the G1/S phase by upregulating p21 protein and inhibiting the expression of TGF-β1 and MMP2, promoting the occurrence of apoptosis and inhibiting the proliferation of human BC T24 cells. Moreover, decorin increased the adhesion of tumor cells in vitro, and effectively inhibited cell metastasis.
Decorin regulated the expression of TGF-β1 and MMP2 through p21 protein, promoted apoptosis and adhesion, and inhibited the proliferation and metastasis of BC cells.
Factors affecting clinical outcomes after IVF-ET for infertile young patients with ovarian endometrioma: A 5-year retrospective cohort study
Authors: Zhou Feng , Zhao Fanxuan , Jin Xiaoying , Li Chao , Zhang Songying 
Published: June 2022

This study aimed to compare ovarian reserve function and outcomes after in vitro fertilization and embryo transfer (IVF-ET) for young women with pelvic endometriosis with or without ovarian endometrioma. We explored the main factors influencing pregnancy outcomes in young patients with endometrioma.
A total of 619 patients ≤38 years of age who underwent IVF-ET in our reproductive center between January 2011 and December 2015 were recruited. Among these patients, 398 had pelvic endometriosis with ovarian endometrioma and 221 had pelvic endometriosis without ovarian endometrioma. Patients underwent ovulation induction during IVF-ET. The general conditions and clinical outcomes of IVF-ET treatment were compared. Key factors affecting the success of IVF-ET treatment for endometriomas were analyzed.
During IVF-ET treatment, the numbers of retrieved oocytes and 2-pronuclei (2PN) embryos in all age groups (P < .01), and the number of 2PN high-quality embryos in patients under 30 years of age was lower in the pelvic endometriosis with ovarian endometrioma group than in the pelvic endometriosis alone group (P < .05). Logistic regression analysis showed the number of antral follicles, basal follicle-stimulating hormone (bFSH) levels, number of oocytes, number of 2PN embryos, and number of 2PN high-quality embryos were significantly related to the successful outcome of IVF-ET. Among these, the number of 2PN high-quality embryos was the only independent predictive factor.
Ovarian endometrioma significantly impairs ovarian reserve function and ultimately affects the therapeutic efficacy of IVF-ET. Obtaining more 2PN high-quality embryos was important for IVF-ET treatment of young patients with ovarian endometriomas.
Functional outcomes of COVID-19 patients with acute ischemic stroke: A prospective, observational, single-center study in North Jordan
Authors: Al Qawasmeh Majdi , Ahmed Yaman B. , Nsour Omar A. , Qarqash Aref A. , Al-Horani Sami S. , Hazaimeh Ethar A. , Jbarah Omar F. , Yassin Ahmed , Aldabbour Belal , Alhusban Ahmed , El-Salem Khalid 
Published: June 2022

We assessed whether stroke severity, functional outcome, and mortality in patients with ischemic stroke differed between patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and those without.
We conducted a prospective, single-center cohort study in Irbid, North Jordan. All patients diagnosed with ischemic stroke and SARS-CoV-2 infection were consecutively recruited from October 15, 2020, to October 16, 2021. We recorded demographic data, vascular risk factors, National Institutes of Health Stroke Scale (NIHSS) score, stroke subtype according to the Trial of ORG 10172 in Acute Stroke Treatment Criteria (TOAST), treatments at admission, and laboratory variables for all patients. The primary endpoint was the functional outcome at 3 months assessed using the modified Rankin Score. Secondary outcomes involved in-hospital mortality and mortality at 3 months.
We included 178 patients with a mean (standard deviation) age of 67.3 (12), and more than half of the cases were males (96/178; 53.9%). Thirty-six cases were coronavirus disease 2019 (COVID-19) related and had a mean (standard deviation) age of 70 (11.5). When compared with COVID-19-negative patients, COVID-19-positive patients were more likely to have a higher median NIHSS score at baseline (6 vs 11; P = .043), after 72 hours (6 vs 12; P = .006), and at discharge (4 vs 16; P < .001). They were also more likely to have a higher median modified Rankin Score after 3 months of follow-up (P < .001). NIHSS score at admission (odds ratio = 1.387, 95% confidence interval = 1.238–1.553]; P < .001) predicted having an unfavorable outcome after 3 months. On the other hand, having a concomitant SARS-CoV-2 infection did not significantly impact the likelihood of unfavorable outcomes (odds ratio = 1.098, 95% confidence interval = 0.270–4.473; P = .896).
The finding conclude that SARS-CoV-2 infection led to an increase in both stroke severity and in-hospital mortality but had no significant impact on the likelihood of developing unfavorable outcomes.