bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D printing of injury-preconditioned secretome/collagen/heparan sulfate scaffolds for neurological recovery after traumatic brain injury in rats
Authors: Liu Xiao-Yin , Chang Zhe-Han , Chen Chong , Liang Jun , Shi Jian-Xin , Fan Xiu , Shao Qi , Meng Wei-Wei , Wang Jing-Jing , Li Xiao-Hong 
Published: December 2022

Abstract
Background
The effects of traumatic brain injury (TBI) can include physical disability and even death. The development of effective therapies to promote neurological recovery is still a challenging problem. 3D-printed biomaterials are considered to have a promising future in TBI repair. The injury-preconditioned secretome derived from human umbilical cord blood mesenchymal stem cells showed better stability in neurological recovery after TBI. Therefore, it is reasonable to assume that a biological scaffold loaded with an injury-preconditioned secretome could facilitate neural network reconstruction after TBI.

Methods
In this study, we fabricated injury-preconditioned secretome/collagen/heparan sulfate scaffolds by 3D printing. The scaffold structure and porosity were examined by scanning electron microscopy and HE staining. The cytocompatibility of the scaffolds was characterized by MTT analysis, HE staining and electron microscopy. The modified Neurological Severity Score (mNSS), Morris water maze (MWM), and motor evoked potential (MEP) were used to examine the recovery of cognitive and locomotor function after TBI in rats. HE staining, silver staining, Nissl staining, immunofluorescence, and transmission electron microscopy were used to detect the reconstruction of neural structures and pathophysiological processes. The biocompatibility of the scaffolds in vivo was characterized by tolerance exposure and liver/kidney function assays.

Results
The excellent mechanical and porosity characteristics of the composite scaffold allowed it to efficiently regulate the secretome release rate. MTT and cell adhesion assays demonstrated that the scaffold loaded with the injury-preconditioned secretome (3D-CH-IB-ST) had better cytocompatibility than that loaded with the normal secretome (3D-CH-ST). In the rat TBI model, cognitive and locomotor function including mNSS, MWM, and MEP clearly improved when the scaffold was transplanted into the damage site. There is a significant improvement in nerve tissue at the site of lesion. More abundant endogenous neurons with nerve fibers, synaptic structures, and myelin sheaths were observed in the 3D-CH-IB-ST group. Furthermore, the apoptotic response and neuroinflammation were significantly reduced and functional vessels were observed at the injury site. Good exposure tolerance in vivo demonstrated favorable biocompatibility of the scaffold.

Conclusions
Our results demonstrated that injury-preconditioned secretome/collagen/heparan sulfate scaffolds fabricated by 3D printing promoted neurological recovery after TBI by reconstructing neural networks, suggesting that the implantation of the scaffolds could be a novel way to alleviate brain damage following TBI.

Graphical abstract
High pulse pressure is associated with an increased risk of diabetes in females but not in males: a retrospective cohort study
Authors: Jia Sheng , Wang Xinyue , Yao Qing , Gao Jian 
Published: December 2022

Abstract
Objective
Accumulating evidence suggests a close relationship between metabolic disturbance and increased arterial stiffness. However, whether there is an association between pulse pressure (PP) and diabetes and how this association might be impacted by sex is not clear.

Methods
A total of 209,635 adult Chinese individuals > 20 years old across 32 sites and 11 cities in China (Shanghai, Beijing, Nanjing, Suzhou, Shenzhen, Changzhou, Chengdu, Guangzhou, Hefei, Wuhan, Nantong) were included in the study; participants were free of diabetes at baseline. In the present study, we analyzed the relationship between PP at baseline and incident diabetes using the Cox proportional hazard model.

Results
During a median follow-up of 2.99 years, a total of 3971 participants (2885 men and 1086 women) developed diabetes, and the incidence was 6.3 per 1000 person-years. With each 10 mmHg increase in PP, the multivariable adjusted hazard ratio (HR) (95% confidence interval) for incident diabetes was 1.117 (1.061, 1.176) in females and 0.981 (0.951, 1.012) in males. Using the lowest quartile of PP as the reference category, the hazard ratio (HR) (95% CI) of the highest quartile of PP for incident diabetes was 1.494 (1.225, 1.822) in females and 0.939 (0.843, 1.045) in males. Smooth plots revealed a significant difference between males and females in the HRs for new-onset diabetes according to PP.

Conclusion
Higher PP was related to future diabetes development in females but not in males and further research is needed to explore the mechanism.
Sex and out-of-hospital cardiac arrest survival: a systematic review
Authors: Lakbar Ines , Ippolito Mariachiara , Nassiri Aviv , Delamarre Louis , Tadger Philippe , Leone Marc , Einav Sharon 
Published: December 2022

Abstract
Background
The literature is unresolved on whether female receive advanced cardiac life support less than do male and on whether female have a survival advantage over male after cardiopulmonary resuscitation.

Methods
We systematically searched PubMed, Embase and Web of Science databases (from inception to 23-April-2022) for papers reporting outcomes in adult male and female after out-of-hospital cardiac arrest. The main study outcome was the rate of adjusted survival to hospital discharge or 30 days. Secondary outcomes included unadjusted survival to hospital discharge and favourable neurological outcome.

Results
A total of 28 studies were included, involving 1,931,123 patients. Female were older than male, their cardiac arrests were less likely to be witnessed and less likely to present with a shockable rhythm. Unadjusted analysis showed that females had a lower likelihood of survival than males (OR 0.68 [0.62–0.74], I2 = 97%). After adjustment, no significant difference was identified between male and female in survival at hospital discharge/30 days (OR 1.01 [0.93–1.11], I2 = 87%). Data showed that male had a significantly higher likelihood of favorable neurological outcome in unadjusted analysis but this trend disappeared after adjustment. Both the primary outcome (adjusted for several variables) and the secondary outcomes were associated with substantial heterogeneity. The variables examined using meta-regression, subgroup and sensitivity analyses (i.e., study type, location, years, population, quality of adjustment, risk of bias) did not reduce heterogeneity.

Conclusions
The adjusted rate of survival to hospital discharge/30 days was similar for male and female despite an initial seeming survival advantage for male. The validity of this finding is limited by substantial heterogeneity despite in-depth investigation of its causes, which raises concerns regarding latent inequalities in some reports nonetheless. Further study on this topic may require inclusion of factors not reported in the Utstein template and in-depth analysis of decision-making processes.
Risk factors and mortality of carbapenem-resistant Klebsiella pneumoniae bloodstream infection in a tertiary-care hospital in China: an eight-year retrospective study
Authors: Chen Jie , Ma Hua , Huang Xiaoming , Cui Yanhui , Peng Wenzhong , Zhu Fei , Ma Shiyang , Rao Minjun , Zhang Peipei , Yang Hang , Su Longxiang , Niu Ruichao , Pan Pinhua 
Published: December 2022

Abstract
Background
The prevalence of carbapenem-resistant Klebsiella pneumoniae bloodstream infection (CRKP-BSI) is increasing worldwide. CRKP-BSI is associated with high rates of morbidity and mortality due to limited antibiotic choices. Here, we aim to identify the prevalence and risk factors for infection and mortality of CRKP BSI.

Methods
This was a retrospective study of the past data from January 1st, 2012 to December 31st, 2019 of adult patients with KP-BSI in Xiangya Hospital, China.

Results
Among the 706 incidences included in this study, 27.4% of them (212/753) being CR-KP strains. The occurrence of CRKP-BSI was increased from 20.69 to 37.40% from 2012 to 2019. Hematologic malignancies and ICU acquired infection were identified to be substantial risk factors of carbapenem resistance. The overall 28-day mortality rates of CRKP-BSI patients was significantly higher than that of CSKP-BSI (P < 0.001). Logistic regression analysis identified severe sepsis or septic shock incidents, inadequate empirical antimicrobial therapy and corticosteroids use preceding infection onset as the independent predictors of 28-day mortality of CRKP-BSI patients. However, high dose carbapenem combination therapy was identified as anticipated factors of low 28-day mortality.

Conclusion
The occurrence of CRKP-BSI was significantly increased during the study period. Hematologic malignancies and ICU acquired infection were associated with the development of CRKP BSI. Severe sepsis or septic shock incidents, inadequate empirical antimicrobial therapy and corticosteroids use preceding infection onset caused significant increase of mortality rates in CRKP-BSI patients. High dose carbapenem combination therapy was associated with better outcome.
Improved quantification of catechin and epicatechin in red rice (Oryza sativa L.) using stable isotope dilution liquid chromatography-mass spectrometry
Authors: Kim Tae Jin , Kim Ye Jin , Seo Woo Duck , Park Sang Un , Kim Jae Kwang 
Published: December 2022

AbstractEpimerization can change the catechin content and composition of samples during extraction and analytical analyses. To control the effect of epimerization, we developed a novel and stable isotope dilution liquid chromatography-mass spectrometry (LC–MS) method using catechin-2,3,4-13C3 and epicatechin-2,3,4-13C3 as stable-isotope-labeled internal standards (SIL-ISs). When the SIL-ISs were used, the catechin and epicatechin contents were stable (104–109% and 100–109% of the initial concentration, respectively) despite long storage times. In contrast, when L-2-chlorophenylalanine was used as an internal standard, catechin and epicatechin concentrations of 88–97% and 164–277% of the initial concentration, respectively, were obtained after long storage times. Furthermore, the least significant epimerization effect and highest extractability were observed when extraction was performed at 70 ℃ for 30 min. The recoveries for red rice using the developed isotope dilution LC–MS method at two different concentrations were between 100.72 and 118.67%, with relative standard deviations less than 3.67%.
Experimental and Numerical Investigations on Crack Intersection and Propagation of Concrete Structures
Authors: Wang Siyao , Zhuang Mei-Ling , Xue Xiang 
Published: December 2022

AbstractThis study presents experimental and numerical methods to reveal concrete structures’ crack propagation and intersection laws under static and dynamic loads. Firstly, a numerical simulation method was established using the user-defined material subroutines to solve the free crack surface contact problem of concrete structures. Secondly, three-point bending tests of concrete beams containing double cracks of different approaching angles were carried out based on the digital image correlation (DIC) technology to study the intersection and propagation of cracks. Finally, the fracture processes of double-crack concrete beams and concrete gravity dams with and without a longitudinal crack were simulated and analyzed under static and dynamic loads. The numerical results were compared with the test results to verify the effectiveness and accuracy of the proposed method in simulating crack intersection and propagation in concrete structures. Results indicate that the crack intersection affects the fracture path of concrete structures, weakens their bearing capacity, and accelerates the failure of the structures. The proposed simulation method provides an effective technical approach for crack propagation prediction and safety evaluation of engineering structures.
The impact of COVID-19 on the treatment of opioid use disorder in carceral facilities: a cross-sectional study
Authors: Saunders Elizabeth C. , Satcher Milan F. , Monico Laura B. , McDonald Ryan D. , Springer Sandra A. , Farabee David , Gryczynski Jan , Nyaku Amesika , Reeves Donald , Kunkel Lynn E. , Schultheis Alysse M. , Schwartz Robert P. , Lee Joshua D. , Marsch Lisa A. , Waddell Elizabeth Needham 
Published: December 2022

AbstractWhile the COVID-19 pandemic disrupted healthcare delivery everywhere, persons with carceral system involvement and opioid use disorder (OUD) were disproportionately impacted and vulnerable to severe COVID-associated illness. Carceral settings and community treatment programs (CTPs) rapidly developed protocols to sustain healthcare delivery while reducing risk of COVID-19 transmission. This survey study assessed changes to OUD treatment, telemedicine use, and re-entry support services among carceral and CTPs participating in the National Institute on Drug Abuse (NIDA)-funded study, Long-Acting Buprenorphine vs. Naltrexone Opioid Treatments in Criminal Justice System-Involved Adults (EXIT-CJS) study. In December 2020, carceral sites (n = 6; median pre-COVID 2020 monthly census = 3468 people) and CTPs (n = 7; median pre-COVID 2020 monthly census = 550 patients) participating in EXIT-CJS completed a cross-sectional web-based survey. The survey assessed changes pre- (January–March 2020) and post- (April–September 2020) COVID-19 in OUD treatment, telemedicine use, re-entry supports and referral practices. Compared to January–March 2020, half of carceral sites (n = 3) increased the total number of persons initiating medication for opioid use disorder (MOUD) from April–September 2020, while a third (n = 2) decreased the number of persons initiated. Most CTPs (n = 4) reported a decrease in the number of new admissions from April–September 2020, with two programs stopping or pausing MOUD programs due to COVID-19. All carceral sites with pre-COVID telemedicine use (n = 5) increased or maintained telemedicine use, and all CTPs providing MOUD (n = 6) increased telemedicine use. While expansion of telemedicine services supported MOUD service delivery, the majority of sites experienced challenges providing community support post-release, including referrals to housing, employment, and transportation services. During the COVID-19 pandemic, this small sample of carceral and CTP sites innovated to continue delivery of treatment for OUD. Expansion of telemedicine services was critical to support MOUD service delivery. Despite these innovations, sites experienced challenges providing reintegration supports for persons in the community. Pre-COVID strategies for identifying and engaging individuals while incarcerated may be less effective since the pandemic. In addition to expanding research on the most effective telemedicine practices for carceral settings, research exploring strategies to expand housing and employment support during reintegration are critical.
Variations in the TAS2R38 gene among college students in Hubei
Authors: Wang Xiaojun , Wang Lin , Xia Mengwei , Teng Feng , Chen Xuejiao , Huang Rufeng , Zhou Jiahao , Xiao Juan , Zhai Lihong 
Published: December 2022

Abstract
Background
The bitter taste receptor gene TAS2R38 is a member of the human TAS2R gene family. Polymorphisms in TAS2R38 affect the ability to taste the bitterness of phenylthiourea (PTC) compounds, thus affecting an individual’s food preference and health status.

Methods
We investigated polymorphisms in the TAS2R38 gene and the sensitivity to PTC bitterness among healthy Chinese college students in Hubei province. The association of TAS2R38 polymorphisms and PTC sensitivity with body mass index (BMI), food preference, and health status was also analyzed. A total of 320 healthy college students were enrolled (male: 133, female: 187; aged 18–23 years). The threshold value method was used to measure the perception of PTC bitterness, and a questionnaire was used to analyze dietary preferences and health status. Polymerase chain reaction (PCR) was used to analyze polymorphisms at three common TAS2R38 loci (rs713598, rs1726866, and rs10246939).

Results
In our study population, 65.00% of individuals had medium sensitivity to the bitterness of PTC; in contrast, 20.94% were highly sensitive to PTC bitterness, and 14.06% were not sensitive. For the TAS2R38 gene, the PAV/PAV and PAV/AAI diplotypes were the most common (42.19% and 40.63%, respectively), followed by the homozygous AVI/AVI (8.75%) and PAV/AVI (5.00%) diplotypes.

Conclusion
There was a significant correlation between the sensitivity to PTC bitterness and sex, but there was no correlation between the common diplotypes of TAS2R38 and gender. Polymorphisms in the TAS2R38 gene were associated with the preference for tea, but not with one’s native place, BMI, health status, or other dietary preferences. There was no significant correlation between the perception of PTC bitterness and one’s native place, BMI, dietary preference, or health status. We hope to find out the relationship between PTC sensitivity and TAS2R38 gene polymorphisms and dietary preference and health status of Chinese population through this study, providing relevant guidance and suggestions for dietary guidance and prevention of some chronic diseases in Chinese population.
The digitalisation in chartering business: special reference to the role of e-bill of lading in the bulk and liner markets
Authors: Plomaritou Evi , Jeropoulos Sotiris 
Published: December 2022

AbstractPrevious research offering a comprehensive overview of digitalisation in maritime transport is limited. In this paper, the authors use several cases to examine digitalisation’s role in the chartering business. The applications of innovative digitalisation to enhance efficiency in the shipping business are presented analytically. Special emphasis is given to the role of the e-bill of lading in the bulk and liner markets. The advantages, disadvantages and legal barriers of the e-bill of lading are examined thoroughly for both markets (bulk and liner markets). The research follows a qualitative case study approach. It shows that although digital technologies offer important advantages in the chartering business, many legal barriers still need to be overcome. The findings fill the gap in the literature and assist maritime professionals and shipping companies in understanding the necessity of digitalisation in chartering business.
The perceived affordances of simulation-based learning: online student teachers’ perspectives
Authors: Dittrich Lynn , Aagaard Toril , Hjukse Hjørdis 
Published: December 2022

AbstractIn this study, we explore the perceived affordances of simulation-based learning (SBL) among online student teachers in Norway. We investigated how simulations mediate professional learning when coupled with educator-led debriefings and peer-led focus groups. Using an exploratory qualitative design, we examined 21 pre-service teachers’ perspectives on their experiences of this SBL intervention. We analysed peer-led focus group data, as well as written reflection logs. Our findings reveal that despite experienced technical challenges, the overall intervention afforded work-life relevant practice, prompting collaborative reflection. In addition to expert feedback, the peer-led focus group discussions are found to be a key component of the SBL design, as these enabled collaborative reflection over a shared practice teaching experience. The findings imply that SBL can be used to bridge the theory-practice gap, a recurring challenge in the field. Consequently, SBL has transformative potential in teacher education.
First report of preimplantation genetic diagnosis of mucopolysaccharidoses IVA and HLA typing for hematopoietic stem cell transplantation
Authors: Khordadpoor Deilamani Faravareh , Akbari Mohammad Taghi 
Published: December 2022

Abstract
Background
Mucopolysaccharidoses IVA is an autosomal recessive lysosomal storage disease resulting in skeletal and cartilage dysplasia. Hematopoietic stem cell transplantation is a good therapeutic option for MPS IV. Here we report the first application of PGD test for MPS IVA and HLA with the purpose of HSCT for the affected son in a family with consanguineous marriage. Haplotype analysis of linked STR markers in GALNS gene and HLA loci as well as variant detection by cycle sequencing were included in our PGD test.

Results
Two out of nine embryos were transferrable. The second embryo transfer was successful and resulted in the pregnancy of one healthy and HLA matched girl.

Conclusions
Preimplantation genetic diagnosis could be considered as a noninvasive clinical option for families with a mucopolysaccharidoses IVA patient to have a healthy child that is HLA-matched with the patient in need of hematopoietic stem cell transplantation. In lack of an appropriate hematopoietic stem cell donor the importance of preimplantation genetic diagnosis is much more significant too.
Long-term recovery of Mexican spotted owl nesting habitat after fire in the Lincoln National Forest, New Mexico
Authors: Durboraw Tara D. , Boal Clint W. , Fleck Mary S. , Gill Nathan S. 
Published: December 2022

Abstract

Background
Dry mixed-conifer forests of the southwestern United States are experiencing rapid, anthropogenically driven fire regime change. Prior to the Euro-American settlement, most of these forests experienced frequent surface fires but are now vulnerable to uncharacteristically large, high-severity fires. Fire directly influences the structure and composition of these forests and, in turn, the wildlife that inhabit them. Changing fire regimes result in a certain decline of some species and uncertain consequences for others. The Mexican spotted owl (Strix occidentalis lucida) is a federally listed threatened species of particular note in southwestern mixed-conifer forests. High-severity fire is cited as the owl’s primary threat in the revised species recovery plan, but uncertainties surround the impacts of high-severity fire on the habitat of the threatened owl, particularly across a timeframe longer than a few years. Our objective was to explore the long-term (100-year) effects of fire severity on elements of forest structure vital for Mexican spotted owl nesting. We quantified structural attributes for nest/roost habitat across mixed-conifer forests that burned at varying severity levels and time periods in the last century. We then examined the drivers of structural attributes by detecting statistical differences between severity classes and time periods through permutational multivariate analysis of variance.

Results
High-severity fire has the strongest deleterious impact on elements of forest structure (total basal area, percent medium tree basal area, percent large tree basal area, large tree density, and canopy cover) vital to Mexican spotted owl nesting, and although the structural differences between severity classes diminish with time, it took ≥ 80–100 years to reach the structural conditions desired for Mexican spotted owl nesting after stand-replacing fires. The most important attribute measured, canopy cover, required 90–100 years after high-severity fires to reach levels most suitable for Mexican spotted owls in the Lincoln National Forest.

Conclusions
As fires increase in frequency, severity, and size compared to the last century, the Lincoln National Forest is projected to face an overall decrease in the structural conditions needed for Mexican spotted owl nesting habitat in this region. Short intervals between uncharacteristically high-severity fires in particular pose an imminent threat to nesting habitat.