bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overview of meningococcal epidemiology and national immunization programs in children and adolescents in 8 Western European countries
Authors: Pinto Cardoso Gaëlle , Lagrée-Chastan Marion , Caseris Marion , Gaudelus Joël , Haas Hervé , Leroy Jean-Philippe , Bakhache Pierre , Pujol Jean-François , Werner Andreas , Dommergues Marie-Aliette , Pauquet Emilie , Pinquier Didier 
Published: November 2022

BackgroundIn Europe, meningococcal (Men) vaccines are available against 5 of the 6 serogroups responsible of nearly all cases of invasive meningococcal disease (IMD). Meningococcal vaccination has been introduced in the national immunization programs (NIPs) for children and adolescents of numerous European countries, but with no consistent strategy across countries.ObjectivesTo describe IMD epidemiology, NIPs, and vaccination coverage rates (VCRs) in children and adolescents in 8 Western European countries.MethodsEpidemiological data (from 1999 to 2019), NIPs regarding meningococcal vaccination status, and VCRs were collected from the European Centre for Disease Prevention and Control (ECDC) and/or national websites.ResultsMenB was the most common serogroup. In Belgium, Spain, France, the Netherlands, the United Kingdom (UK), and Portugal, incidence was greater for MenW than MenC. In 2019, MenB risk was covered in 2 countries (Italy, UK). MenC risk was covered in all countries, via MenC only (countries: N = 3), MenACWY only (N = 2), or MenC (infants/children) and MenACWY (adolescents) (N = 3) vaccination. VCRs were higher in children than adolescents.ConclusionOur study confirmed the diversity of NIPs, including in neighboring European countries with similar factors like economic resources and epidemiological risk, thus indicating that other factors underlie NIPs. Convergence toward a more common immunization program including MenACWY and MenB vaccination would promote equity and safe travel regarding infectious diseases for young people, and possibly improve the understanding of vaccination by patients and healthcare professionals.
The role and mechanism of NLRP3 inflammasome-mediated astrocyte activation in dehydrocorydaline against CUMS-induced depression
Authors: Fang Yu , Guo Hong , Wang Qiannan , Liu Congcong , Ge Shuyi , Yan Bohua 
Published: November 2022

Background: Depression is a common and potentially life-threatening mental illness, and currently, there is a lack of effective treatment. It has been reported that dehydrocorydaline (DHC) can inhibit monoamine transporter uptake in depressed CUMS mice, but more possible mechanisms of action remain to be further studied.Methods: C57BL/6 mice were exposed to chronic unpredictable mild stress (CUMS) for five consecutive weeks. The mice were administrated with dehydrocorydaline or fluoxetine (FLU) for four consecutive weeks. Behavioral tests including sucrose preference test (SPT), tail suspension test (TST), and forced swimming test (FST) were applied. In parallel, hematoxylin and eosin (H&amp;E) staining and Nissl staining were used to explore the effect of DHC on pathological changes in the hippocampus. The concentrations of depression-related factors (5-HT and DA) and inflammatory factors (TNF-α, IL-6, and IL-1β) in the hippocampus and serum were assessed by ELISA assay. NLRP3 inflammasome pathway-related proteins (NLRP3, IL-18, IL-1 IL-1α, and caspase-1) were detected by western blot. The activation of microglia and astrocytes was subjected to immunofluorescent staining. Additionally, microglia were treated with DHC (100 mg/L) for 24 h following incubation with 100 ng/ml LPS for 12 h. ov-NC or ov-NLRP3 plasmid was transfected into microglia 6 h before LPS induction for exploring the effect of NLRP3 overexpression on DHC-inhibited microglia activation. Then, conditioned media of microglia were collected from each group, followed by intervention of astrocytes for 24 h to explore the effect of NLRP3 overexpression of microglia on astrocyte activation.Results:In vivo administration of DHC was found to ameliorate depressive-like behaviors and attenuate neuron damage of CUMS mice. DHC increased neurotransmitter concentration, reduced the proinflammatory factor levels, attenuated NLRP3 inflammasome activation, and decreased A1 astrocyte and microglia activation in the hippocampus of CUMS mice. Furthermore, in vivo results showed that activated microglia induced activation of A1 astrocytes but not A2 astrocytes.Conclusion: Taken together, we provided evidence that DHC exhibited antidepressive effects on CUMS mice possibly via NLRP3 inflammasome-mediated astrocyte activation.
Exploration of the effect of Celastrol on protein targets in nasopharyngeal carcinoma: Network pharmacology, molecular docking and experimental evaluations
Authors: Ling Junjun , Huang Yu , Sun Zhen , Guo Xiaopeng , Chang Aoshuang , Pan Jigang , Zhuo Xianlu 
Published: November 2022

Background: Celastrol, an important extract of Tripterygium wilfordii, shows strong antitumor activity in a variety of tumors including nasopharyngeal carcinoma (NPC). However, little is known about its targets in NPC. We aimed to screen the key gene targets of Celastrol in the treatment of NPC by means of in silico analyses (including network pharmacology and molecular docking) and experimental evaluations.Methods: The main target genes of Celastrol and the genes related to NPC were obtained by retrieving the relevant biological databases, and the common targets were screened. Protein-protein interaction analysis was used to screen the hub genes. Then, a “compound-target-disease” network model was created and molecular docking was used to predict the binding of Celastrol to the candidate hub proteins. Afterward, the expression changes of the candidate genes under the administration of Celastrol were verified in vitro and in vivo.Results: Sixty genes common to Celastrol and NPC were screened out, which may be related to numerous biological processes such as cell proliferation, apoptosis, and tube development, and enriched in various pathways such as PI3K- Akt, EGFR tyrosine kinase inhibitor resistance, and Apoptosis. The tight binding ability of the candidate hub proteins (TNF, VEGFA, and IL6) to Celastrol was predicted by molecular docking [Docking energy: TNF, −6.08; VEGFA,−6.76; IL6,−6.91(kcal/mol)]. In vitro experiments showed that the expression of TNF and VEGFA decreased while the expression of IL6 increased in NPC cells (CNE2 and HONE1) treated with Celastrol. In vivo experiments suggested that Celastrol significantly reduced the weight and volume of the transplanted tumors in tumor-bearing mice in vivo. The expression of TNF, VEGFA, and IL6 in the transplanted tumor cells could be regulated by using Celastrol, and the expression trends were consistent with the in vitro model.Conclusion: Several gene targets have been filtered out as the core targets of Celastrol in the treatment of NPC, which might be involved in a variety of signaling pathways. Hence, Celastrol may exert its anti-NPC activity through multiple targets and multiple pathways, which will provide new clues for further research. Future experiments are warranted to validate the findings.
Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum
Authors: Li Chunchun , Ul Haq Inzamam , Khurshid Aroosa , Tao Yan , Quandahor Peter , Zhou Jing-Jiang , Liu Chang-Zhong 
Published: November 2022

Insect chitinases play a crucial part to digest chitin in the exoskeleton during the molting process. However, research on insect chitinase related to the environmental stress response is very limited. This study was the first conducted to expression analysis of chitinase- related genes in A. pisum under abiotic stresses. Here, we identified five chitinase-like proteins (ApIDGF, ApCht3, ApCht7, ApCht10 and ApENGase), and clustered them into five groups (group II, III, V, Ⅹ, and ENGase). Developmental expression analysis revealed that the five A. pisum chitinase-related genes were expressed at whole developmental stages with different relative expression patterns. When aphids were exposed to various abiotic stresses including temperature, insecticide and the stress 20-hydroxyecdysone (20E), all five chitinase genes were differentially expressed in A. pisum. The results showed that insecticide such as imidacloprid down-regulated the expression of these five Cht-related genes. Analysis of temperature stress of A. pisum chitinase suggested that ApCht7 expression was high at 10°C, which demonstrates its important role in pea aphids under low temperature. Conversely, ApCht10 was more active under high temperature stress, as it was significantly up-regulated at 30°C. Besides, 20E enhanced ApCht3 and ApCht10 expression in A. pisum, but reduced ApCht7 expression. These findings provide basic information and insights for the study of the role of these genes under abiotic stress, which advances our knowledge in the management of pea aphids under multiple stresses.
Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm
Authors: Esuma Williams , Eyoo Oscar , Gwandu Francisca , Mukasa Settumba , Alicai Titus , Ozimati Alfred , Nuwamanya Ephraim , Rabbi Ismail , Kawuki Robert 
Published: November 2022

IntroductionThe intrinsic high heterozygosity of cassava makes conventional breeding ineffective for rapid genetic improvement. However, recent advances in next generation sequencing technologies have enabled the use of high-density markers for genome-wide association studies, aimed at identifying single nucleotide polymorphisms (SNPs) linked to major traits such as cassava mosaic disease (CMD) resistance, dry matter content (DMC) and total carotenoids content (TCC). A number of these trait-linked SNPs have been converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers for downstream application of marker assisted selection.MethodsWe assayed 13 KASP markers to evaluate their effectiveness in selecting for CMD, DMC and TCC in 1,677 diverse cassava genotypes representing two independent breeding populations in Uganda.ResultsFive KASP markers had significant co-segregation with phenotypes; CMD resistance (2), DMC (1) and TCC (2), with each marker accounting for at least 30% of the phenotypic variation. Markers located within the chromosomal regions for which strong marker-trait association loci have been characterised (chromosome 12 markers for CMD, chromosome 1 markers for DMC and TCC) had consistently superior ability to discriminate the respective phenotypes.DiscussionThe results indicate varying discriminatory abilities of the KASP markers assayed and the need for their context-based use for MAS, with PSY2_572 particularly effective in selecting for high TCC. Availing the effective KASP markers on cost-effective genotyping platforms could facilitate practical implementation of marker-assisted cassava breeding for accelerated genetic gains for CMD, DMC and provitamin A carotenoids.
Response of physiological characteristics of ecological restoration plants to substrate cement content under exogenous arbuscular mycorrhizal fungal inoculation
Authors: Shu Qian , Xia Dong , Ma Yueyang , Zhang Yang , Luo Ting , Ma Jiaxin , Liu Fang , Yan Shuxing , Liu Daxiang 
Published: November 2022

IntroductionIn order to solve the inhibition of alkaline environment on plants growth at the initial stage of Eco-restoration of vegetation concrete technology, introducing AMF into vegetation concrete substrate is an effective solution. MethodsIn this study, Glomus mosseae (GM), Glomus intraradices (GI) and a mixture of two AMF (MI) were used as exogenous inoculation agents. Festuca elata and Cassia glauca were selected as host plants to explore the relationship between the physiological characteristics of plants and the content of substrate cement under exogenous inoculation of AMF.ResultsThe experiment showed that, for festuca elata, the maximum mycorrhizal infection rates of inoculation with GM, MI were when the cement contents ranged 5–8% and that of GI inoculation was with the cement contents ranging 5–10%. Adversely, for Cassia glauca, substrate cement content had little effect on the root system with the exogenous inoculation of AMF. Compared with CK, the effects of AMF inoculation on the physiological characteristics of the two plants were different. When the cement content was the highest (10% and 8% respectively), AMF could significantly increase(p&lt;0.05) the intercellular CO2 concentration (Ci) of Festuca elata. Moreover, for both plants, single inoculation was more effective than mixed inoculation. When the cement content was relatively low, the physiological characteristics of Cassia glauca were promoted more obviously by the inoculation of GI. At higher cement content level, inoculation of GM had a better effect on the physiological characteristics of the two plants. ConclusionThe results suggest that single inoculation of GM should be selected to promote the growth of Festuca elata and Cassia glauca in higher alkaline environment.
Potential distribution of three types of ephemeral plants under climate changes
Authors: Lan Zhang , Huiliang Liu , Hongxiang Zhang , Yanfeng Chen , Lingwei Zhang , Kudusi Kawushaer , Taxmamat Dilxadam , Yuanming Zhang 
Published: November 2022

BackgroundArid and semi-arid regions account for about 40% of the world’s land surface area, and are the most sensitive areas to climate change, leading to a dramatic expansion of arid regions in recent decades. Ephemeral plants are crucial herbs in this area and are very sensitive to climate change, but it is still unclear which factors can determine the distribution of ephemeral plants and how the distribution of ephemeral plants responds to future climate change across the globe.AimsUnderstanding the impact of climate change on ephemeral plant distribution is crucial for sustainable biodiversity conservation.MethodsThis study explored the potential distribution of three types of ephemeral plants in arid and semi-arid regions (cold desert, hot desert, and deciduous forest) on a global scale using the MaxEnt software. We used species global occurrence data and 30 environmental factors in scientific collections.ResultsOur results showed that (1) the average value of the area under the receiver operating curve (AUC) of each species was higher than 0.95, indicating that the MaxEnt model’s simulation accuracy for each species was good; (2) distributions of cold desert and deciduous forest species were mainly determined by soil pH and annual mean temperature; the key factor that determines the distribution of hot desert species was precipitation of the driest month; and (3) the potential distribution of ephemeral plants in the cold desert was increased under one-third of climate scenarios; in the hot desert, the potential suitable distribution forAnastatica hierochunticawas decreased in more than half of the climate scenarios, butTrigonella arabicawas increased in more than half of the climate scenarios. In deciduous forests, the ephemeral plantCrocus alatavicusdecreased in nearly nine-tenths of climate scenarios, andGagea filiformiswas increased in 75% of climate scenarios.ConclusionsThe potential suitable distributions of ephemeral plants in the different ecosystems were closely related to their specific adaptation strategies. These results contribute to a comprehensive understanding of the potential distribution pattern of some ephemeral plants in arid and semi-arid ecosystems.
A meta-analysis of the effects of cognitive behavioral therapy on quality of life and negative emotions of informal cancer caregivers
Authors: Zhou Shuang , Wang Yumei , Wang Qiang , Yang Guodong , Ren Huipeng , Bao Yanping 
Published: November 2022

ObjectiveThis study aimed to systematically review the effect of cognitive behavioral therapy (CBT) in terms of improving the quality of life (QOL) and negative emotion of informal cancer caregivers.MethodsPubMed, Cochrane Library, EMBASE, Web of Science, MEDLINE, China National Knowledge Infrastructure (CNKI), and China Biology Medicine disc (CBMdisc) were searched from the database establishment to October 2021. Literature screening, data extraction, and quality evaluation were conducted based on inclusion and exclusion criteria. Stata 14.0 software was used for data analysis.ResultsA total of 17 randomized controlled trials (RCTs) involving 2,348 cancer informal caregivers (CGs) were included in this study, with an overall loss rate of 13.3%. Meta-analysis showed no statistically significant difference in the impact of CBT on QOL (SMD = 0.28, 95%CI: −0.09–0.65, P &lt; 0.001). However, the improvement of depression in CBT intervention group was significantly better than that in control group (SMD = −0.32, 95%CI: −0.56 to −0.07, P = 0.010). The HADS subgroup showed differences in depression scores (SMD = −0.80, 95%CI: −1.30 to −0.29, P = 0.002). The overall effect of CBT on anxiety was statistically different, the improvement of anxiety in CBT intervention group was significantly better than that in control group (SMD = −0.36, 95%CI: −0.720–0.004, P = 0.047).ConclusionsCBT had a positive effect on reducing depression and anxiety in informal cancer caregivers, and the effect on quality of life was not statistically significant, but showed a positive trend.Systematic Review Registrationhttps://inplasy.com/generate-invoice/, identifier: INPLASY202230120.
Impact of COVID-19 pandemic on catheter ablation in China: A spatiotemporal analysis
Authors: Jiang Jiang , Zhao Shuang , Cheng Chendi , Lin Na , Li Ping , Ning Xiaohui , Zhang Shu 
Published: November 2022

BackgroundThe COVID-19 pandemic has significantly impacted routine cardiovascular health assessments and services. We aim to depict the temporal trend of catheter ablation (CA) and provide experience in dealing with the negative impact of the COVID-19 pandemic.MethodsData on CA between January 2019, and December 2021, were extracted from the National Center for Cardiovascular Quality Improvement platform. CA alterations from 2019 to 2021 were assessed with a generalized estimation equation.ResultsA total of 347,924 patients undergoing CA were included in the final analysis. The CA decreased remarkably from 122,839 in 2019 to 100,019 (−18.58%, 95% CI: −33.40% to −3.75%, p = 0.02) in 2020, and increased slightly to 125,006 (1.81%, 95% CI: −7.01% to 3.38%, p = 0.49) in 2021. The CA experienced the maximal reduction in February 2020 (−88.78%) corresponding with the peak of monthly new COVID-19 cases and decreased by 54.32% (95%CI: −71.27% to −37.37%, p &lt; 0.001) during the 3-month lockdown and increased firstly in June 2020 relative to 2019. Since then, the CA in 2020 remained unchanged relative to 2019 (−0.06%, 95% CI: −7.01% to 3.38%, p = 0.98). Notably, the recovery of CA in 2021 to pre-COVID-19 levels was mainly driven by the growth of CA in secondary hospitals. Although there is a slight increase (2167) in CA in 2021 relative to 2019, both the absolute number and proportion of CA in the top 50 hospitals nationwide [53,887 (43.09%) vs. 63,811 (51.95%), p &lt; 0.001] and top three hospitals in each province [66,152 (52.73%) vs. 72,392 (59.28%), p &lt; 0.001] still declined significantly.ConclusionsThe CA experienced a substantial decline during the early phase of the COVID-19 pandemic, and then gradually returned to pre-COVID-19 levels. Notably, the growth of CA in secondary hospitals plays an important role in the overall resumption, which implies that systematic guidance of secondary hospitals with CA experience may aid in mitigating the negative impact of the COVID-19 pandemic.
What do we know about the effect of night-shift work on cardiovascular risk factors? An umbrella review
Authors: Boini Stephanie , Bourgkard Eve , Ferrières Jean , Esquirol Yolande 
Published: November 2022

ObjectiveTo evaluate the existing evidence on the effect of night-shift work and its subtypes (permanent and rotating) on cardiovascular risk factors: diabetes, lipid disorders, being overweight, hypertension, smoking habits, sedentariness, and occupational psychosocial stressors.MethodA Web of Sciences and Cochrane review library search was conducted to identify systematic reviews with or without meta-analysis dealing with the quantification of the link between night-shift work and the studied cardiovascular risk factors in working populations. We used the AMSTAR 2 to evaluate the quality of each review. The main results of the included systematic reviews were compiled in a summary structured around the different cardiovascular risk factors.ResultsAfter selection, 33 systematic reviews were included: nine for diabetes, four for lipid disorders, nine for being overweight, four for hypertension, two for smoking habits, three for occupational psychosocial stressors and two for sedentariness. The results confirmed an excess risk of diabetes of about 10% regardless of the type of night work. A stated excess risk of being overweight at around 25% was also highlighted for shift workers overall, which could reach 38% among night-shift workers. An increased risk of obesity, estimated at 5% for night-shift workers and at 18% for rotating shift workers, was observed. An excess risk of hypertension was estimated at around 30% when considering the broad definition of shift work and when night periods were included in rotating shifts. The literature provided inconsistent results for the link between lipid disorders and night-shift work. Shift workers appeared to be more likely to smoke. The link between shift work and occupational psychosocial stressors was scarcely explored in the available studies. Sedentariness was scarcely considered in systematic reviews, which prevents any firm conclusions.ConclusionThe consequences of night work in terms of diabetes, being overweight/obesity and hypertension are established. Monitoring of these cardiovascular risk factors for these night-shift workers could be implemented by practitioners. In contrast, the links with lipid disorders, sedentariness, smoking habits, and occupational psychosocial stressors warrant further investigation.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021275212, PROSPERO (ID CRD42021275212)
Staging study of single-channel sleep EEG signals based on data augmentation
Authors: Ling Huang , Luyuan Yao , Xinxin Li , Bingliang Dong 
Published: November 2022

IntroductionAccurate sleep staging is an essential basis for sleep quality assessment and plays an important role in sleep quality research. However, the occupancy of different sleep stages is unbalanced throughout the sleep process, which makes the EEG datasets of different sleep stages have a class imbalance, which will eventually affect the automatic assessment of sleep stages.MethodIn this paper, we propose a Residual Dense Block and Deep Convolutional Generative Adversarial Network (RDB-DCGAN) data augmentation model based on the DCGAN and RDB, which takes two-dimensional continuous wavelet time–frequency maps as input, expands the minority class of sleep EEG data and later performs sleep staging by Convolutional Neural Network (CNN).Results and discussionThe results of the CNN classification comparison test with the publicly available dataset Sleep-EDF show that the overall sleep staging accuracy of each stage after data augmentation is improved by 6%, especially the N1 stage, which has low classification accuracy due to less original data, also has a significant improvement of 19%. It is fully verified that data augmentation by improving the DCGAN model can effectively improve the classification problem of the class imbalance sleep dataset.