bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Circulating Proteins with Death or Lung Transplant in Patients with Idiopathic Pulmonary Fibrosis in the IPF-PRO Registry Cohort
Authors: Todd Jamie L. , Neely Megan L. , Overton Robert , Mulder Hillary , Roman Jesse , Lasky Joseph A. , de Andrade Joao A. , Gulati Mridu , Huang Howard , Leonard Thomas B. , Hesslinger Christian , Noth Imre , Belperio John A. , Flaherty Kevin R. , Palmer Scott M. ,   the IPF-PRO Registry investigators
Published: January 2022

AbstractIdiopathic pulmonary fibrosis (IPF) is a progressive and ultimately fatal disease with a variable clinical course. Biomarkers that predict patient outcomes are needed. We leveraged data from 300 patients in the multicenter IPF-PRO Registry to determine associations between circulating proteins and the composite outcome of respiratory death or lung transplant. Plasma collected at enrollment was analyzed using aptamer-based proteomics (1305 proteins). Over a median follow-up of 30.4 months, there were 76 respiratory deaths and 26 lung transplants. In unadjusted univariable analyses, 61 proteins were significantly associated with the outcome (hazard ratio > 2 or < 0.5, correctedp ≤ 0.05). In multivariable analyses, a set of 4 clinical measures and 47 unique proteins predicted the probability of respiratory death or lung transplant with an optimism-corrected C-index of 0.76. Our results suggest that select circulating proteins strongly associate with the risk of mortality in patients with IPF and confer information independent of clinical measures.
Orexin A peptidergic system: comparative sleep behavior, morphology and population in brains between wild type and Alzheimer’s disease mice
Authors: Zhao Peng , You Yaqian , Wang Zhe , Zhou Yanjun , Chai Gaoshang , Yan Gen , Jin Zhewu , Wang Qing , Sun Hongxu 
Published: January 2022

AbstractSleep disturbance is common in patients with Alzheimer’s disease (AD), and orexin A is a pivotal neurotransmitter for bidirectionally regulating the amyloid-β (Aβ) deposition of AD brain and poor sleep. In the present study, we examined the characteristic of sleep–wake architecture in APPswe/PSldE9 (APP/PS1) and Aβ-treated mice using electroencephalogram (EEG) and electromyographic (EMG) analysis. We compared the expression of orexin A, distribution, and morphology of the corresponding orexin A-positive neurons using innovative methods including three-dimensional reconstruction and brain tissue clearing between wild type (WT) and APP/PS1 mice. Results from our study demonstrated that increased wakefulness and reduced NREM sleep were seen in APP/PS1 and Aβ treated mice, while the expression of orexin A was significantly upregulated. Higher density and distribution of orexin A-positive neurons were seen in APP/PS1 mice, with a location of 1.06 mm–2.30 mm away from the anterior fontanelle compared to 1.34 mm–2.18 mm away from the anterior fontanelle in WT mice. These results suggested that the population and distribution of orexin A may play an important role in the progression of AD.
The changing face of maxillofacial trauma during the 2020 COVID-19 lockdowns in Melbourne, Australia
Authors: Nhongo Sipho Simon , Sklavos Anton , Lee Kai , Chan Steven T. F. , Austin Stephen 
Published: January 2022

Abstract
Purpose
To compare the incidence, aetiology, and patterns of maxillofacial fracture presentations during the various stages of the 2020 Melbourne COVID-19 lockdown restrictions to periods outside lockdown in 2019 and 2020.

Methods
This is a retrospective study of 344 subjects. The patterns of facial trauma presentations to a tertiary hospital in metropolitan Melbourne during the 2020 COVID-19 restrictions were compared to periods with no restrictions over 22 months from March 2019 to December 2020.

Results
The incidence of maxillofacial fractures decreased by 28% during lockdown (0.41 vs. 0.57 injuries/day, P = 0.0003). Falls overtook interpersonal violence as the leading cause of fractures (44% of lockdown presentations vs. 25.7% of presentations outside lockdown, P = 0.002), while sporting injuries dropped drastically (4% vs. 17.1%, P = 0.005). Lockdowns saw an increase in the proportion of female patients (40% vs. 26.8%, P = 0.03) and a fivefold increase in proportion of domestic violence-related fractures (6.7% vs. 1.1%, P = 0.006). Alcohol-related injuries decreased significantly (11% vs. 21%, P = 0.03).

Conclusions
While restrictions reduced rates of interpersonal violence and alcohol-related maxillofacial trauma, there was a higher proportion of injuries to females, increased falls, and domestic violence-related injuries.
Study of the biological characteristics of human umbilical cord mesenchymal stem cells after long-time cryopreservation
Authors: Zhang Mingqi , Zhao Yan , Wang Le , Zheng Yuqiang , Yu Hui , Dong Xiaoming , He Wei , Yin Zhengqin , Wang Zhuoshi 
Published: January 2022

Abstract
Human umbilical cord mesenchymal stem cells (hUC-MSCs) have considerable potential in cell therapy. Cryopreservation represents the gold standard in cell storage, but its effect on hUC-MSCs is still not well understood. The aim of this study was to investigate the effect of one year of cryopreservation and thawing on the biological characteristics of hUC-MSCs from the same donors. Fresh hUC-MSCs were cryopreserved in commercial freezing medium (serum-free CellBanker 2) at passage 2. After one year of cryopreservation, the hUC-MSCs were thawed and subcultured to passage 4. The comparison was performed in terms of followings: cell count, viability, morphology, proliferation capacity, differentiation potential and chromosomal stability. The total cell count and viability of hUC-MSCs before and after one year of cryopreservation were 1 × 107 and 96.34% and 0.943 × 107 and 93.81%, respectively. Cryopreserved and fresh hUC-MSCs displayed a similar cell doubling times, expressed the markers CD73, CD90, CD105 and were negative for the markers CD34, CD45, and HLA-DR. Karyotypes were found to be normal after one year of cryopreservation. The trilineage differentiation properties were maintained after cryopreservation. However, when compared to freshly isolated hUC-MSCs from the same donor, cryopreserved hUC-MSCs exhibited decreased expression of osteogenesis- and chondrogenesis-related genes including Runx2, Sox9, and Col1a1, and increased expression of adipogenesis-related genes. These results demonstrated that cryopreservation did not affect cell morphology, surface marker expression, cell viability, proliferative capacity, or chromosomal stability. However, the osteogenic and chondrogenic differentiation capacities of cryopreserved hUC-MSCs were slightly reduced compared with those of fresh cells from the same donor.

Graphical abstract
Importance of IGF-I levels in IVF: potential relevance for growth hormone (GH) supplementation
Authors: Gleicher Norbert , Darmon Sarah K. , Molinari Emanuela , Patrizio Pasquale , Barad David. H. 
Published: January 2022

AbstractPurposeGrowth hormone (GH) supplementation in association with in vitro fertilization (IVF) is worldwide again increasing, even though study outcomes have been discrepant. Since GH acts via insulin-like growth factor-1 (IGF-1), its utilization in IVF would only seem to make sense with low IGF-1. We, therefore, determined whether IGF-I levels affect IVF outcomes.MethodsRetrospectively, 302 consecutive first fresh, non-donor IVF cycles were studied, excluding patients on GH supplementation. Patients were divided into 3 subgroups: IGF-1 in lower 25th percentile (group A, < 132 ng/mL,n = 64); 25th–75th percentile (B, 133–202 ng/mL,n = 164), and upper 25th percentile (C, > 202 ng/mL,n = 74). IGF-1 was tested immunochemiluminometric with normal range at 78–270 ng/mL. Because of the study patients’ adverse selection and low pregnancy chances, the main outcome measure for the study was cycle cancellation. Secondary outcomes were oocyte numbers, embryos transferred, pregnancies, and live births.ResultsGroup A was significantly older than B and C (P = 0.019). IGF-1 decreased with increasing age per year by 2.2 ± 0.65 ng/mL (P = 0.0007). FSH was best in group B and worst in A (trend,P = 0.085); AMH was best in B and worst in A (N.S.). Cycle cancellations were lowest in C (11.6%) and highest in A (25.0%;P = 0.042). This significance further improved with age adjustment (P = 0.021). Oocytes, embryo numbers, pregnancies, and live birth rates did not differ, though oocyte numbers trended highest in B.ConclusionsHere presented results support the hypothesis that IGF-1 levels affect IVF outcomes. GH treatments, therefore, may be effective only with low IGF-1.
Spontaneous Fluctuation-Symmetry Breaking and the Landauer Principle
Authors: Buffoni Lorenzo , Campisi Michele 
Published: January 2022

AbstractWe study the problem of the energetic cost of information erasure by looking at it through the lens of the Jarzynski equality. We observe that the Landauer bound, $$\langle W \rangle \ge kT \ln 2$$

⟨
W
⟩
≥
k
T
ln
2

, on average dissipated work $$\langle W \rangle $$

⟨
W
⟩

associated to an erasure process, literally emerges from the underlying second law bound as formulated by Kelvin, $$\langle W \rangle \ge 0$$

⟨
W
⟩
≥
0

, as consequence of a spontaneous breaking of the Crooks–Tasaki fluctuation-symmetry, that accompanies logical irreversibility. We illustrate and corroborate this insight with numerical simulations of the process of information erasure performed on a 2D Ising ferromagnet.
Detection and Sequencing of Multiple Human Norovirus Genotypes from Imported Frozen Raspberries Linked to Outbreaks in the Province of Quebec, Canada, in 2017
Authors: Raymond Philippe , Paul Sylvianne , Perron André , Bellehumeur Christian , Larocque Émilie , Charest Hugues 
Published: January 2022

AbstractHuman noroviruses are among the main causes of acute gastroenteritis worldwide. Frozen raspberries have been linked to several norovirus food-related outbreaks. However, the extraction of norovirus RNA from frozen raspberries remains challenging. Recovery yields are low and PCR inhibitors limit the sensitivity of the detection methodologies. In 2017, 724 people from various regions of the Province of Quebec, Canada, were infected by noroviruses and the outbreak investigation pointed to frozen raspberries as a putative source. A new magnetic silica bead approach was used for the extraction of viruses from different outbreak samples. The RNA extracts were tested by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and five samples were confirmed positive for norovirus by RT-qPCR amplicon sequencing. A multiplex long-range two-step RT-PCR approach was developed to amplify norovirus ORF2 and ORF3 capsid genes from the positive frozen raspberry RNA extracts and other sequencing strategies were also explored. These capsid genes were sequenced by Next-Generation Sequencing. Phylogenetic analyses confirmed the presence of multiple genotypes (GI.3, GI.6, and GII.17) and intra-genotype variants in some of the frozen raspberry samples. Variants of genotype GI.3 and GI.6 had 100% homology with sequences from patient samples. Similar strains were also reported in previous outbreaks. Confirmation approaches based on sequencing the norovirus capsid genes using Next-Generation Sequencing can be applied at trace level contaminations and could be useful to assess risk and assist in source tracking.
A novel compact 4-channel beam splitter based on a Kösters-type prism
Authors: Greiner J. , Laux U. 
Published: January 2022

AbstractWe introduce a novel compact 4-channel beam splitter which is based on a combination of dichroic coatings and internal total reflection, similar in concept to the interference double prism invented by Kösters 90 years ago [1]. Used with a rapidly-slewing 50 cm telescope in space, this would allow to double the presently known gamma-ray bursts at high (> 5) redshift within 2 years.
Migraine Is Associated with High Risk of Erectile Dysfunction: A Systematic Review and Cumulative Analysis
Authors: He Weicheng , Yang Yi , Liang Hongyi , Huang Zhonghua , Jiang Jiehong 
Published: January 2022

ABSTRACTBackgroundMigraine, a common chronic primary headache, has been found to be associated with a high risk of erectile dysfunction (ED).AimThe present study aims to summarize all the evidence related to this topic and demonstrate a quantified result on the association between migraine and ED, which has not been reported in the literature.MethodsMEDLINE, Excerpta Medica Database, and Cochrane Library were systematically searched for identifying the eligible studies (2000–2021). This study was registered in the PROSPERO (ID: CRD42021248013).OutcomesThe combined effects were synthesized with the relative risks (RR) or standard mean differences (SMD) with 95% confidence intervals (CI).Results6 trials with a total of 51,657 participants were included, of which 6,175 were men with migraine. The pooled analysis indicated that migraine was associated with a significantly higher risk of ED as compared to the non-migraine general population (RR = 1.63, 95%CI: 1.34 to 2.0, P &lt; .001). Consistently, men with migraine have a significantly lower IIEF-5 score than healthy controls (SMD = -3.64, 95%CI: -6.4 to -0.89, P = .01). Stratification analysis on the mean age indicated that the association between migraine and ED was much stronger in the migraine patients with age &lt; 40 years (RR = 32.29, 95% CI: 6.41–162.64, P &lt; .001; I2 = 0.0 %, P = .837) than in those with age &gt; 40 years (RR = 1.75, 95% CI: 1.11–2.78, P = .017; I2 = 89.2%, P = .002). Sensitivity analysis indicated that no single study had dominated the combined RR and the heterogeneity.Clinical ImplicationsED is a common disease among migraine men, especially those patients whose age is under 40 years old. It shows a 32-fold increased risk of ED compared to the healthy controls. Migraine-induced ED may be correlated with multiple factors, that is, chronic illnesses, chronic pain, and psychosocial causes (like anxiety and depression). Since phosphodiesterase-5 inhibitors (ie, sildenafil) might induce or exacerbate migraine, thus it is not recommended to prescribe these drugs for patients with migraine-mediated ED.ConclusionThe present study provides evidence that migraine is associated with a significantly high risk of ED, especially in those aged &lt; 40 years. The pathophysiological mechanisms of this action deserve further study.
Reversible data hiding for JPEG images with a cascaded structure
Authors: Zhan Wuyue , Yao Heng 
Published: January 2022

AbstractSince Joint Photographic Experts Group is a typical image compression standard, so the reversible data hiding research on Joint Photographic Experts Group has received a lot of attention. There are two main research methods in Joint Photographic Experts Group‐reversible data hiding: the method based on modifying DCT coefficients and the method based on variable length coding mapping. The disadvantage of the first one is that the visual loss is always inevitable during the DCT‐coefficient modification process, while the second method often has insufficient embedding capacity and excessive file expansion. In order to solve the above problems, this paper proposes a cascaded reversible data hiding scheme, which builds a distortion minimisation scheme based on the DCT coefficients, and then cascades a file expansion suppression scheme based on the variable length coding mapping. For a particular case during the payload distribution, in this paper, all the secret information is embedded into DCT coefficients with a distortion minimisation scheme, and then the auxiliary information generated in the previous process is embedded in the variable length coding sequence with a scheme to optimise the file size expansion. The experimental results demonstrate the superiority of the proposed method in high‐quality factor circumstances in terms of visual quality and file expansion.
Ende gut, alles gut?
Authors: Heeser Alexandra-Corinna 
Published: January 2022

ZusammenfassungLernen hat sich aufgrund von Corona verändert – digitales Lernen ist seit der Pandemie gefragter denn je. Doch ist diese Form besser oder schlechter als die altehrwürdigen Präsenzveranstaltungen? Sowohl als auch.
Optimized Flow Cytometry Strategy for Phenotyping Intramuscular Leukocytes: Application to the Evaluation of Myopathological Processes
Authors: Saidj Tassadit , Baba Amer Yasmine , Plonquet Anne , Henry Adeline , Souvannanorath Sarah , Relaix Frederic , Beldi-Ferchiou Asma , Authier François Jérôme 
Published: January 2022

Abstract
Phenotyping intramuscular immune cells is essential for the characterization of dysimmune/inflammatory myopathies (DIM). Flow cytometry (FC) is the most reliable technique for analyzing leukocyte subpopulations and evaluating their activation levels. We developed a purely mechanical protocol for extracting cells from muscle tissue allowing us to preserve cell surface epitopes and determined its applicability to experimental pathology in mice and myopathological diagnosis in human. Skeletal muscle regeneration in mice was associated with a transient enrichment of macrophages (CD11bhighGr-1+), myeloid dendritic cells (CD3−C8+CD11bhigh), CD8+ T cells (CD3+C8+), and NK cells (CD3− CD11bhighNKp46+). In murine models of inherited muscle dystrophies, leukocytes represented 23%–84% of intramuscular mononuclear cells, with a percentage of CD8+ T cells (4%–17%) mirroring that of all CD45+ cells, while MDCs remained a minority. In human 16 samples (DIM: n = 9; nonimmune conditions: n = 7), DIM was associated with intramuscular recruitment of CD8+ T cells, but not CD4+ T cells and NK cells. FC allowed concomitant quantification of HLA-DR, CD25, CD38, and CD57 activation/differentiation biomarkers and showed increased activation levels of CD4+ and CD8+ T cells in DIM. In conclusion, FC is an appropriate method for quantifying intramuscular leukocyte subpopulations and analyzing their activation states.
Inhibition of Ferroptosis Attenuates Glutamate Excitotoxicity and Nuclear Autophagy in a CLP Septic Mouse Model
Authors: Xie Zhenxing , Xu Mang , Xie Jie , Liu Tao , Xu Xie , Gao Wei , Li Zhanfei , Bai Xiangjun , Liu Xinghua 
Published: January 2022

ABSTRACT
Sepsis-associated encephalopathy (SAE) often manifests in severe diffuse cerebral dysfunction due to an aberrant systemic immune response to infection. The underlying pathophysiology of SAE is not entirely understood but is likely a multifactorial process that involves disruption in cell death mechanism. Ferroptosis is a novel form of programmed cell death characterized by iron accumulation and lipid peroxidation, leading to inflammatory cascade and glutamate release. We hypothesized that ferroptosis is involved in the glutamate-mediated excitotoxic neuron injury during the uncontrolled neural inflammatory process of SAE. Inhibiting ferroptosis with ferrostatin-1 (Fer-1) could alleviate glutamate excitotoxicity and reduce neuron death of SAE, potentially improving prognosis. We found that in the cecal ligation and puncture (CLP) sepsis model, ferroptosis occurred increasingly in the cerebrum, characterized by glutathione-dependent antioxidant enzyme glutathione peroxidase 4 (GPX4) inactivation, transferrin upregulation, mitochondria shrink and malondialdehyde (MDA) increased. Fer-1 treatment downregulated cerebral ferroptosis and alleviated glutamate excitotoxicity via dampening system xc-(SXC) and glutamate receptor N-methyl-D-asperate receptor subunit 2. Combined with an observed reduction in calcium transporter PLCG and PLCB activation, these processes ultimately protected the integrities of synapses and neurons during SAE. Fer-1 treatment also rescued sepsis-induced nuclear autophagy and improved the behaviors of tail suspension test and novel object recognition test in septic mice. Conclusively, our results suggested that inhibition of ferroptosis could attenuate glutamate excitotoxicity and SAE outcomes.
Clinical Characteristics and Outcomes of Neutropenic Sepsis: A Multicenter Cohort Study
Authors: Na Soo Jin , Oh Dong Kyu , Park Sunghoon , Lee Yeon Joo , Hong Sang-Bum , Park Mi-Hyun , Ko Ryoung-Eun , Lim Chae-Man , Jeon Kyeongman 
Published: January 2022

ABSTRACT

Background:
Sepsis is a leading cause of mortality in patients with neutropenia; however, data on whether neutropenic sepsis is associated with distinct clinical characteristics and outcomes are limited. Thus, this study was designed to clarify the clinical characteristics and outcomes of patients with neutropenic sepsis compared with those of patients without neutropenic sepsis diagnosed based on the Third International Consensus Definitions for Sepsis and Septic Shock criteria.

Methods:
We analyzed data from the Korean Sepsis Alliance, a nationwide prospective multicenter cohort study evaluating the clinical characteristics, management, and outcomes of patients with sepsis from September 2019 to February 2020. Eligible patients were divided into the neutropenic (absolute neutrophil count of less than 1,500/mL) and non- neutropenic groups. The characteristics and outcomes were compared between the two groups.

Results:
During the study period, 2,074 patients were enrolled from 16 tertiary referral or university-affiliated hospitals. Of them, 218 (10.5%) had neutropenia. The neutropenia group was younger and had a lower proportion of patients with chronic diseases compared with the non-neutropenia group. However, solid tumors (50.0% vs. 34.1%; P
> 0.001) and hematological malignancies (40.8% vs. 3.8%; P
< 0.001) were more common in the neutropenia group. The neutropenia group had a higher incidence of septic shock (43.6% vs. 22.9%; P
< 0.001) and higher Sequential Organ Failure Assessment score (7 vs. 5; P
< 0.001) than the nonneutropenia group. However, no significant differences in microbiologically confirmed infections and its pathogen distribution and the incidence of multidrug resistance were observed between the two groups. The neutropenic group had a higher hospital mortality than the non-neutropenic group (42.2% vs. 26.3%; P
< 0.001), and the Kaplan-Meier survival curve demonstrated a significant difference in survival within 1 week after diagnosing sepsis (log-rank test, P = 0.002). The incidence of adverse events during intensive care unit admission was not different between the two groups. Among hospital survivors, the neutropenic group was more frequently discharged to home (72.2% vs. 57.8%; P = 0.002).

Conclusions:
Neutropenic sepsis is associated with a higher-grade organ dysfunction during the diagnosis of sepsis and higher mortality without difference in the pathogen isolated.