bluuebunny/crossref_metadata_2025_split huggingface dataset:
An intersectional analysis of LncRNAs and mRNAs reveals the potential therapeutic targets of Bi Zhong Xiao Decoction in collagen-induced arthritis rats
Authors: He Cailin , Wang Yang , Wen Yuqi , Li Teng , Hu En , Zeng Siqing , Yang Bo , Xiong Xingui 
Published: September 2022

AbstractBackgroundBi Zhong Xiao decoction (BZXD), a traditional Chinese herbal formula, has been used clinically for many years to treat rheumatoid arthritis (RA). Both clinical and experimental studies have revealed that BZXD is effective in treating RA, but the mechanism remains unclear. In this study, we aimed to explore the mechanism of efficacy of BZXD through transcriptomic analysis of lncRNA and mRNA.MethodsThe combination method of ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry was used to assess the quality of BZXD. The efficacy of BZXD in treating collagen-induced arthritis (CIA) was evaluated by clinical assessment, weight changes, hematoxylin–eosin and safranin o-fast green staining, and Micro-CT. Arraystar rat lncRNA-mRNA chip technology was used to determine the lncRNA and mRNA expression profiles of the Control, CIA and BZXD groups, and to screen gene expression profiles related to the curative effect of BZXD. A lncRNA-mRNA co-expression network was constructed for the therapeutic efficacy genes. Through GO function and KEGG pathway enrichment analysis, the biological functions and signaling pathways of therapeutic efficacy genes were determined. Based on fold change and functional annotation, key differentially expressed lncRNAs and mRNAs were selected for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation. The functions of lncRNAs targeting mRNAs were verified in vitro.ResultsWe demonstrated that BZXD could effectively reverse bone erosion. After BZXD treatment, up to 33 lncRNAs and 107 mRNAs differentially expressed genes were reversely regulated by BZXD. These differentially expressed lncRNAs are mainly involved in the biological process of the immune response and are closely related to the ECM-receptor interaction, MAPK signaling pathway, Focal adhesion, Ras signaling pathway, Antigen processing and presentation, and Chemokine signaling pathway. We identified four lncRNAs (uc.361−, ENSRNOT00000092834, ENSRNOT00000089244, ENSRNOT00000084631) and three mRNAs (Acvr2a, Cbx2, Morc4) as potential therapeutic targets for BZXD and their microarray data consistent with the RT-qPCR. In vitro experiments confirmed that silencing the lncRNAs ENSRNOT00000092834 and ENSRNOT00000084631 reversed the expression of target mRNAs.ConclusionsThis study elucidates the possible mechanism of BZXD reversing bone erosion in CIA rats from the perspective of lncRNA and mRNA. To provide a basis and direction for further exploration of the mechanism of BZXD in treating RA.
Mortality of patients with hospital-onset sepsis in hospitals with all-day and non-all-day rapid response teams: a prospective nationwide multicenter cohort study
Authors: Hyun Dong-gon , Lee Su Yeon , Ahn Jee Hwan , Huh Jin Won , Hong Sang-Bum , Koh Younsuck , Lim Chae-Man ,   the Korean Sepsis Alliance (KSA) Investigators, Oh Dong Kyu , Suh Gee Young , Jeon Kyeongman , Ko Ryoung-Eun , Cho Young-Jae , Lee Yeon Joo , Lim Sung Yoon , Park Sunghoon , Heo Jeongwon , Lee Jae-myeong , Kim Kyung Chan , Lee Yeon Joo , Chang Youjin , Jeon Kyeongman , Lee Sang-Min , Hong Suk-Kyung , Cho Woo Hyun , Kwak Sang Hyun , Lee Heung Bum , Ahn Jong-Joon , Seong Gil Myeong , Lee Song-I , Park Sunghoon , Park Tai Sun , Lee Su Hwan , Choi Eun Young , Moon Jae Young 
Published: September 2022

AbstractBackgroundHospital-onset sepsis is associated with a higher in-hospital mortality rate than community-onset sepsis. Many hospitals have implemented rapid response teams (RRTs) for early detection and timely management of at-risk hospitalized patients. However, the effectiveness of an all-day RRT over a non-all-day RRT in reducing the risk of in-hospital mortality in patient with hospital-onset sepsis is unclear. We aimed to determine the effect of the RRT’s operating hours on in-hospital mortality in inpatient patients with sepsis.MethodsWe conducted a nationwide cohort study of adult patients with hospital-onset sepsis prospectively collected from the Korean Sepsis Alliance (KSA) Database from 16 tertiary referral or university-affiliated hospitals in South Korea between September of 2019 and February of 2020. RRT was implemented in 11 hospitals, of which 5 (45.5%) operated 24-h RRT (all-day RRT) and the remaining 6 (54.5%) had part-day RRT (non-all-day RRT). The primary outcome was in-hospital mortality between the two groups.ResultsOf the 405 patients with hospital-onset sepsis, 206 (50.9%) were admitted to hospitals operating all-day RRT, whereas 199 (49.1%) were hospitalized in hospitals with non-all-day RRT. A total of 73 of the 206 patients in the all-day group (35.4%) and 85 of the 199 patients in the non-all-day group (42.7%) died in the hospital (P = 0.133). After adjustments for co-variables, the implementation of all-day RRT was associated with a significant reduction in in-hospital mortality (adjusted odds ratio 0.57; 95% confidence interval 0.35–0.93;P = 0.024).ConclusionsIn comparison with non-all-day RRTs, the availability of all-day RRTs was associated with reduced in-hospital mortality among patients with hospital-onset sepsis.
Remimazolam besylate versus propofol for long-term sedation during invasive mechanical ventilation: a pilot study
Authors: Tang Yun , Yang Xiaobo , Yu Yuan , Shu Huaqing , Yuan Yin , Liu Hong , Zou Xiaojing , Yuan Shiying , Shang You 
Published: September 2022

AbstractObjectiveThe aim of this study was to evaluate the efficacy and safety of remimazolam besylate compared with propofol in maintaining mild-to-moderate sedation in patients receiving long-term mechanical ventilation.MethodsIn this single-centered randomized pilot study, adult patients mechanically ventilated longer than 24 h were randomized to receive remimazolam besylate or propofol. The target sedation range was − 3 to 0 on the Richmond Agitation and Sedation Scale (RASS). The primary outcome was the percentage of time in the target sedation range without rescue sedation. The secondary outcomes were ventilator-free days at day 7, the length of ICU stay and 28-day mortality.ResultsThirty patients were assigned to each group. No difference was identified between the remimazolam group and propofol group in median age [60.0 (IQR, 51.5–66.3) years vs. 64.0 (IQR, 55.0–69.3) years, respectively,p = 0.437] or the median duration of study drug infusion [55.0 (IQR, 28.3–102.0) hours vs. 41.0 (IQR, 24.8–74.3) hours, respectively,p = 0.255]. The median percentage of time in the target RASS range without rescue sedation was similar in remimazolam and propofol groups [73.2% (IQR, 41.5–97.3%) vs. 82.8% (IQR, 65.6–100%),p = 0.269]. No differences were identified between the two groups in terms of ventilator-free days at day 7, length of ICU stay, 28-day mortality or adverse events.ConclusionsThis pilot study suggested that remimazolam besylate was effective and safe for long-term sedation in mechanically ventilated patients compared with propofol.
Study protocol for the management of impacted maxillary central incisors: a multicentre randomised clinical trial: the iMAC Trial
Authors: Seehra Jadbinder , DiBiase Andrew T. , Patel Shruti , Stephens Rachel , Littlewood Simon J. , Spencer Richard J. , Frawley Tom , Benson Philip E. , Ireland Anthony J. , Parvizi Farnaz , Atack Nikki , Kidner Giles , Wojewodka Gabriella , Ward Christopher , Papageorgiou Spyridon N. , Newton Jonathon T. , Cobourne Martyn T. 
Published: September 2022

Abstract
Background
Failure of eruption of the maxillary permanent incisor teeth usually presents in the mixed dentition between the ages of 7 and 9 years. Missing and unerupted maxillary incisors can be regarded as unattractive and have a potentially negative impact on facial and dental aesthetics. The presence of a supernumerary tooth (or odontoma) is commonly responsible for failed eruption or impaction of the permanent maxillary incisors. The primary objective of this trial is to investigate the success of eruption associated with maxillary incisor teeth that have failed to erupt because of a supernumerary tooth in the anterior maxilla.

Methods
This protocol describes an interventional multicentre two-arm randomised clinical trial. Participants meeting the eligibility criteria will be randomised (unrestricted equal participant allocation [1:1]) to either space creation with an orthodontic appliance, removal of the supernumerary tooth and application of direct orthodontic traction or space creation with an orthodontic appliance, removal of the supernumerary tooth and monitoring. The primary outcome of this trial is to determine the prevalence of successfully erupted maxillary central permanent incisors at 6 months following removal of the supernumerary tooth. Secondary outcome measures include (1) the effect of initial tooth position (assessed radiographically) on time taken for the tooth to erupt, (2) time taken to align the unerupted tooth to the correct occlusal position, (3) gingival aesthetics and (4) changes in the self-reported Oral Health Related-Quality of Life (OHRQoL) (pre-and post-treatment).

Discussion
There is a lack of high-quality robust prospective studies comparing the effectiveness of interventions to manage this condition. Furthermore, the UK national clinical guidelines have highlighted a lack of definitive treatment protocols for the management of children who present with an unerupted maxillary incisor due to the presence of a supernumerary tooth. The results of this trial will inform future treatment guidelines for the management of this condition in young children.

Trial registration
ISRCTN Registry ISRCTN12709966. Registered on 16 June 2022.
Role of DNA dioxygenase Ten-Eleven translocation 3 (TET3) in rheumatoid arthritis progression
Authors: Kawabe Akio , Yamagata Kaoru , Kato Shigeaki , Nakano Kazuhisa , Sakata Kei , Tsukada Yu-ichi , Ohmura Koichiro , Nakayamada Shingo , Tanaka Yoshiya 
Published: September 2022

Abstract
Background
Rheumatoid arthritis (RA) patients present with abnormal methylation patterns in their fibroblast-like synoviocytes (FLS). Given that DNA demethylation is critical for producing DNA methylation patterns, we hypothesized that DNA demethylation may facilitate RA progression. Therefore, we designed this study to examine the role of DNA dioxygenase family, Ten-Eleven translocation (TET1/2/3), in the pathological process of RA.

Methods
Synovial tissues and FLS were obtained from patients with RA and Osteoarthritis. K/BxN serum-induced arthritis was induced in Wild-type (WT) and TET3 heterozygous-deficient (TET3+/−) C57BL/6 mice.

Results
We found that both TET3 and 5-hydroxymethylcytosine (5hmC) were upregulated in synovitis tissues from RA patients and confirmed this upregulation in the cultured FLS derived from synovitis tissues. Tumor necrosis factor α (TNFα) upregulated TET3 and 5hmC levels in cultured FLS, and the stimulated FLS exhibited high cell mobility with increased transcription of cellular migration-related factors such as C-X-C motif chemokine ligand 8 (CXCL8) and C-C motif chemokine ligand 2 (CCL2) in a TET3-dependent manner. In addition, TET3 haploinsufficiency lowered RA progression in a mouse model of serum-induced arthritis.

Conclusions
Based on these findings, we can assume that TET3-mediated DNA demethylation acts as an epigenetic regulator of RA progression.
Development of a 64Cu-labeled CD4+ T cell targeting PET tracer: evaluation of CD4 specificity and its potential use in collagen-induced arthritis
Authors: Clausen Anne Skovsbo , Christensen Camilla , Christensen Esben , Cold Sigrid , Kristensen Lotte Kellemann , Hansen Anders Elias , Kjaer Andreas 
Published: September 2022

Abstract
Background
CD4+ T cells are central inflammatory mediators in the pathogenesis of autoimmune rheumatoid arthritis (RA), as they are one of the dominating cell types in synovial inflammation. Molecular imaging of CD4+ T cells has potential role for early detection and monitoring of RA. Here, we developed a new radiotracer for in vivo immunoPET imaging of murine CD4+ T cells and tested it in the collagen-induced arthritis (CIA) mouse model of human RA.

Results
The tracer, [64Cu]Cu-NOTA-CD4-F(ab)’2 ([64Cu]Cu-NOTA-CD4), was generated from F(ab)’2 fragments of R-anti-mouse CD4 antibodies conjugated to the 2-S-(isothiocyanatbenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA) chelator and radiolabeled with copper-64. Accumulation of the tracer and isotype control was evaluated in the CIA model and mice receiving whole-body irradiation (WBI) (5 Gy). The potential of [64Cu]Cu-NOTA-CD4 for response assessment was evaluated in CIA induced mice treated with dexamethasone (DXM). Imaging data were compared with flow cytometry and immunohistochemistry (IHC) of inflammatory cells including CD4+ T cells. [64Cu]Cu-NOTA-CD4 showed increased accumulation in T cell-rich tissues compared with isotype control (p < 0.0001). In addition, reduced accumulation of [64Cu]Cu-NOTA-CD4 was observed in T cell-depleted tissue (p < 0.0001). Flow cytometry and IHC confirmed the increased infiltration of CD4+ T cells in CIA mice.

Conclusions
We developed and evaluated a new radiotracer, [64Cu]Cu-NOTA-CD4, for immunoPET imaging of murine CD4+ T cells. [64Cu]Cu-NOTA-CD4 was successfully synthesized by F(ab)’2 fragments of R-anti-mouse CD4 antibodies conjugated to a chelator and radiolabeled with copper-64. We found that our novel CD4 PET tracer can be used for noninvasive visualization of murine CD4+ T cells.
Trade-offs in German wind energy expansion: building bridges between different interests, values and priorities
Authors: Reitz Sybille , Goshen Lauren , Ohlhorst Dörte 
Published: September 2022

AbstractBackgroundTo achieve climate targets, a transition to low-carbon energy production is necessary. However, conflicts between different interests, values and priorities, particularly at the community level, can constrain this transition. This paper aims to analyze lines of conflict and opportunities to build bridges between conflicting interests in the expansion of wind energy in Germany at the local level, to achieve successful implementation of wind energy projects.ResultsOur analysis of four cases of local-level wind energy projects in Germany shows that limited local options for action reinforce the need for local actors to maximize the benefits of energy transition projects. In addition to the conflict over scarce space, the lines of conflict at the local level run primarily along the dimensions of costs and benefits, winners and losers. Real or perceived procedural and distributive injustices had the potential to fuel resistance to wind energy projects in the analyzed cases. However, wind energy projects were successfully implemented despite the presence of local opposition.ConclusionsThe results show that, by integrating procedural and distributive justice into the project planning and implementation and offering tailored solutions, community support for expansion of renewable energy projects can be enhanced. The paper advances the concept of societal ownership (“gesellschaftliche Trägerschaft”), which suggests the willingness of members of a community to tolerate decisions even when some conflicts related to the decision remain unresolved. Societal ownership is presented as an alternative to the concept of simple acceptance; it implies a more positive, more supportive community attitude, where members aim to address conflict as a normal aspect of decision making. Rather than sweeping alternative opinions aside, the community addresses alternative viewpoints, seeking to achieve greater procedural and distributive justice. In this way, a sense of societal ownership of a project can develop, enhancing its likelihood of success.
Association between abnormal lipid profile and inflammation and progression of myelodysplastic syndrome to acute leukemia
Authors: Qiao Wei , Young Elliana , Feng Chun , Liu Suyu , Jin Jeff , Noor Laila , Rojas Hernandez Cristhiam M. , Borthakur Gautam , Gorlova Olga , Afshar-Kharghan Vahid 
Published: September 2022

AbstractClonal hematopoiesis of indeterminate potential (CHIP) is associated with a small risk of developing hematologic malignancies and a higher risk of cardiovascular diseases (CVD). We asked whether the reverse correlation exists and cardiometabolic risk factors have an impact on the progression of myelodysplastic syndrome (MDS) to acute myeloid leukemia (AML). We investigated the association between abnormal lipid profiles and inflammation in MDS, which shares many genetic mutations with CHIP, and the risk of developing acute leukemia. We examined the medical records of 11071 MDS patients. Among them, 5422 had at least one lipid profile or C-reactive protein (CRP) measurement. In univariate and multivariate analyses, elevated triglyceride and high-sensitive C-reactive protein (HS-CRP) were significantly associated with a diagnosis of acute leukemia in MDS patients. Next, we examined these associations in patients with available MDS prognostic scores (International Prognostic Scoring System, IPSS, or its revised version IPSS/R) (n = 2786 patients). We found that the statistical association between CRP and the progression of MDS to leukemia was independent of other variables in the scoring system. MDS patients with elevated CRP in both the high-risk and low-risk groups had a higher risk of progression to AML than those with a lower CRP. We speculate that inflammation might be a common denominator in developing hematologic malignancies and CVD in patients with clonal hematopoiesis.
Use of Serendipita indica to improve soybean growth, physiological properties, and soil enzymatic activities under different Cd concentrations
Authors: Wang Xiaohui , Fan Xiaoxu , Wang Weidong , Song Fuqiang 
Published: September 2022

Abstract
Background
Plants-microbial technology has been regarded as a popular and applied new technique for the remediation of cadmium (Cd) polluted soils.

Methods
Therefore, a pot experiment was conducted to explore the effect of Serendipita indica (S. indica) on soybean growth and the soil enzyme activities, pH and Cd chemical fractions in the soil in 0, 3, 6, and 9 mg·kg−1 Cd concentrations.

Results
Results reflected that compared to uninoculated treatment, S. indica inoculation can still enhance the dry weight (66.57%) and shoot length (90.35%) and promote the net photosynthesis rate (72.18%), transpiration ratio (80.73%), and stomatal conductance (119.05%) of soybean under 9 mg·kg−1 Cd stress. Furthermore, the soil pH, phosphatase (116.39%), and catalase (4.17%) activities in the S. indica treatments were increased under 3 mg·kg−1 Cd concentration. Meanwhile, S. indica could shift Cd from exchangeable fraction to other stable fractions, primarily decreased Cd contamination degree/risk in 6 mg·kg−1 Cd concentration.

Conclusions
This work suggested that S. indica may be a potential biotechnology for low Cd-contaminated farmland, S. indica can not only alleviate Cd phytotoxicity and promote plant growth but also improve soil quality in Cd-contaminated soils.

Graphical Abstract
Global exposure: an alternative pathway to understanding cultural omnivorousness in East Asian societies
Authors: Du Shichao 
Published: September 2022

AbstractPrevious studies on cultural taste build a class-omnivorousness framework. However, the conceptualization and measure of cultural omnivorousness are highly Western. To examine how cultural omnivorousness is shaped in non-Western societies, this study develops two dimensions of cultural omnivorousness and expands the meaning of social class from socioeconomic status to global exposure. Using data from the East Asian Social Survey, this study finds that the level of global exposure is significantly correlated with vertical cultural omnivorousness (i.e., the appreciation of both highbrow and lowbrow music) in China, Japan, and South Korea; however, the correlation between the level of global exposure and horizontal cultural omnivorousness (i.e., the appreciation of both transnational and traditional music) varies among the three countries. The findings show the diverse nature of cultural consumption in East Asia and challenge the Western discourse in the cultural sphere.
Right thyrocervical trunk rupture after right internal jugular vein puncture: a case report and systematic review of the literature
Authors: Ono Yuko , Ueshima Eisuke , Nakanishi Nobuto , Shinohara Kazuaki , Yamada Isamu , Kotani Joji 
Published: September 2022

Abstract
Background
Thyrocervical trunk rupture is an unusual, but critical, complication associated with central venous catheter (CVC) placement. The management of this complication has not been fully determined because it is rare.

Case presentation
A 53-year-old Japanese woman with anorexia nervosa developed refractory ventricular fibrillation. After returning spontaneous circulation, a CVC was successfully placed at the initial attempt in the right internal jugular vein using real-time ultrasound guidance. Immediately after CVC placement, she developed enlarging swelling around the neck. Contrast-enhanced computed tomography showed massive contrast media extravasation around the neck and mediastinum. Brachiocephalic artery angiography showed a “blush” appearance of the ruptured right thyrocervical trunk. After selective arterial embolization with 33% N-butyl-2-cyanoacrylate, the extravasation completely disappeared and hemostasis was achieved.

Conclusion
Our findings suggest that severe vascular complications arising from CVC placement can occur in patients with a fragile physiological state. Endovascular embolization is an effective treatment for such complications.
Risk factors for wasting among hospitalised children in Nepal
Authors: Inoue Aiko , Dhoubhadel Bhim Gopal , Shrestha Dhruba , Raya Ganendra Bhakta , Hayashi Yumiko , Shrestha Sudeep , Edwards Tansy , Parry Christopher Martin , Ariyoshi Koya , Cox Sharon Elizabeth 
Published: September 2022

Abstract
Background
Malnutrition has various adverse effects in children. This study aimed to determine risk factors for malnutrition among hospitalised children, changes in nutritional status at admission and discharge and effects of use of systematic anthropometric measurement in identification of malnutrition.

Methods
We enrolled 426 children, aged between 6 months and 15 years, admitted to Siddhi Memorial Hospital, Bhaktapur, Nepal, from November 2016 to June 2017. Anthropometric measurements were performed at the time of admission and discharge. Risk factors were assessed by multivariable logistic regression models.

Results
Median age of children was 26 months (IQR: 13–49), and males were 58.7%. The prevalence of wasting was 9.2% (39/426) at admission and 8.5% (36/426) at discharge. Risk factors associated with wasting at admission were ethnic minority (aOR: 3.6, 95% CI 1.2–10.8), diarrhoeal diseases (aOR = 4.0; 95% CI 1.3–11.8), respiratory diseases (aOR: 3.4, 95% CI 1.4–8.1) and earthquake damage to house (aOR = 2.6; 95% CI 1.1–6.3). Clinical observation by care providers identified only 2 out of 112 malnutrition cases at admission and 4 out of 119 cases at discharge that were detected by the systematic anthropometric measurement.

Conclusions
Ethnic minority, diarrhoeal diseases, respiratory infections and house damage due to the earthquake were risk factors associated with wasting. Systematic anthropometric examination can identify significantly more malnourished children than simple observation of care providers.