bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analisis Permasalahan Transportasi di Perkotaan: Studi Kasus pada Kawasan Perkotaan Yogyakarta
Authors: Kurniawan Galih Puji , Shalikhah Salsabila Zahra , Shofiati Hanifah , Azizah Nuha Nur , Mochtar Mahmud 
Published: April 2022

Penelitian ini bertujuan untuk menganalisis permalasahan transportasi di perkotaan studi kasus pada kawasan perkotaan yogyakarta. Adapun jenis penelitian ini adalah penelitian kualitatif dengan sumber data diperoleh dari sumber data primer dan sumber data sekunder. Data penelitian yang terkumpul kemudian dianalisis dengan pendekatan ilmu sains. Dari penelitian yang dilakukan diperoleh hasil bahwa permasalahan transportasi di perkotaan khususnya di Kawasan Perkotaan Yogyakarta disebabkan oleh yang pertama belum adanya sarana dan prasana yang memadai di Kawasan Perkotaan Yogyakarta. Kedua, rendahnya kesadaran masyarakat terhadap pemanfaatan fasilitas lalu lintas dan perlengkapan jalan. Kemudian, di Kawasan Perkotaan Yogyakarta belum mengimplementasikan dengan baik tentang manajemen kebutuhan lalu lintas untuk meningkatkan efesiensi dan efektifitas penggunaan lalu lintas dan mengendalikan pergerakan lalu lintas. Disamping itu, laju pertumbungan penduduk yang cukup tinggi di Kawasan Perkotaan Yogyakarta berakibat pada kemacetan lalu lintas di wilayah tersebut. Permasalahan transportasi yang memiliki eksternalitas lintas daerah seperti daerah Kawasan Perkotaan Yogyakarta tidak mungkin dilakukan dan ditangani oleh daerah sendiri
Kata Kunci: Analisis Permasalahan Perkotaan, Studi Kasus Kawasan, Permasalahan Transportasi
Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium
Authors: Guo Qi , Chang Yajie , Li Jingjie , Zhou Chuanchuan , Huang Rui , Yang Xing , Liu Guihua , Liang Xiaoyan 
Published: April 2022

Stem cell–based therapy plays a pivotal role in the regeneration of damaged endometrium. Previous studies have demonstrated the therapeutic potential of bone marrow mesenchymal stem cells (BMSCs) through diverse administration ways. However, the homing, survival, and differentiation potential of these differently administered BMSCs are poorly defined, and the best route of administration is not well-defined. Herein, we aim to compare the engraftment, retaining time, and therapeutic efficiency of differently administered BMSCs. To achieve this, GFP/Luc-labeled BMSCs administered in two modes were assessed in a thin endometrium rat model: either into the damaged horns directly or through the ipsilateral iliac artery. The retaining time and hemi-quantitative distribution were evaluated by in vivo bioluminescence imaging and immunohistological analysis. Locally administered BMSCs were strongly detected in the abdomen at the first 4 days post treatment but underwent a rapid decrease in luminescent signal afterward and were rarely found 28 days after treatment. In contrast, the retaining time of BMSCs injected through the iliac artery was longer, reflected by more GFP-positive cells detected in the uterine section 28 days post treatment. Differentiation toward endometrial stromal cells was observed. Both routes of administration contributed to the restoration of the damaged endometrium, showing a comparable increase in the endometrial thickness and a decrease in fibrosis. However, more importantly, higher expression of LIF and VEGF, better recruitment, and longer retainment were found in the intra-arterial administration, contributing to the establishment of the optimal administration mode in clinical practice.
Production of Hexanol as the Main Product Through Syngas Fermentation by Clostridium carboxidivorans P7
Authors: Oh Hyun Ju , Ko Ja Kyong , Gong Gyeongtaek , Lee Sun-Mi , Um Youngsoon 
Published: April 2022

The production of hexanol from syngas by acetogens has gained attention as a replacement for petroleum-derived hexanol, which is widely used in the chemical synthesis and plastic industries. However, acetogenic bacteria generally produce C2 compounds (e.g., acetate and ethanol) as the main products. In this study, the gas fermentation conditions favorable for hexanol production were investigated at different temperatures (30–37°C) and CO gas contents (30–70%) in batch gas fermentation. Hexanol production increased from 0.02 to 0.09 g/L when the cultivation temperature was lowered from 37 to 30°C. As the CO content increased from 30 to 70%, the CO consumption rate and hexanol production (yield, titer, and ratio of C6 compound to total products) increased with the CO content. When 70% CO gas was repeatedly provided by flushing the headspace of the bottles at 30°C, the total alcohol production increased to 4.32 g/L at the expense of acids. Notably, hexanol production (1.90 g/L) was higher than that of ethanol (1.20 g/L) and butanol (1.20 g/L); this is the highest level of hexanol produced in gas fermentation to date and the first report of hexanol as the main product. Hexanol production was further enhanced to 2.34 g/L when 2 g/L ethanol was supplemented at the beginning of 70% CO gas refeeding fermentation. Particularly, hexanol productivity was significantly enhanced to 0.18 g/L/day while the supplemented ethanol was consumed, indicating that the conversion of ethanol to acetyl-CoA and reducing equivalents positively affected hexanol production. These optimized culture conditions (gas fermentation at 30°C and refeeding with 70% CO gas) and ethanol supplementation provide an effective and sustainable approach for bio-hexanol production.
Nationwide Initiation of Cardiovascular Risk Treatments During the COVID-19 Pandemic in France: Women on a Slippery Slope?
Authors: Gabet Amélie , Grave Clémence , Tuppin Philippe , Lesuffleur Thomas , Guenancia Charles , Nguyen-Thanh Viêt , Guignard Romain , Blacher Jacques , Olié Valérie 
Published: April 2022

ObjectivesThis study examines the initiation of prescribed medication treatments for cardiovascular risk (antihypertensives, lipid-lowering drugs, oral anticoagulants in atrial fibrillation, and smoking cessation medications) during the COVID-19 pandemic in the French population.MethodsFor each year between 2017 and 2021, we used the French National Insurance Database to identify the number of people with at least one reimbursement for these medications but no reimbursement in the previous 12 months. We computed incidence rate ratios (IRRs) between 2017–2019 and, respectively 2020 and 2021 using Poisson regression adjusted for age and 2017–2019 time trends. We recorded the number of lipid profile blood tests, Holter electrocardiograms, and consultations with family physicians or cardiologists.ResultsIn 2020, IRR significantly decreased for initiations of antihypertensives (−11.1%[CI95%, −11.4%;−10.8%]), lipid-lowering drugs (−5.2%[CI95%, −5.5%;−4.8%]), oral anticoagulants in atrial fibrillation (−8.6%[CI95%, −9.1%;−8.0%]), and smoking cessation medications (−50.9%[CI95%, −51.1%;−50.7%]) compared to 2017–2019. Larger decreases were found in women compared to men except for smoking cessation medications, with the sex difference increasing with age. Similar analyses comparing 2021 to 2017–2019 showed an increase in the initiation of lipid-lowering drugs (+ 11.6%[CI95%, 10.7%;12.5%]) but even lower rates for the other medications, particularly in women. In addition, the 2020 number of people visiting a family physician or cardiologist decreased by 8.4 and 7.4%. A higher decrease in these visits was observed in those over 65 years of age compared to those under 65 years of age. A greater use of teleconsultation was found in women.ConclusionThe COVID-19 pandemic heavily impacted the initiation of medication treatments for cardiovascular risk in France, particularly in women and people over 65 years.
Predictive Value of Left Atrial and Ventricular Strain for the Detection of Atrial Fibrillation in Patients With Cryptogenic Stroke
Authors: Bufano Gabriella , Radico Francesco , D'Angelo Carolina , Pierfelice Francesca , De Angelis Maria Vittoria , Faustino Massimiliano , Pierdomenico Sante Donato , Gallina Sabina , Renda Giulia 
Published: April 2022

Background and AimsCryptogenic stroke (CS) is associated with a high rate of recurrences and adverse outcomes at long-term follow-up, especially due to its unknown etiology that often leads to ineffective secondary prevention. Asymptomatic atrial fibrillation (AF) could play an important pathophysiological role. Some studies have pointed to left atrial (LA) and left ventricular (LV) systolic and diastolic dysfunction as surrogate markers of AF. The aim of the study is to evaluate the relationship between echocardiographic parameters of LA and LV function, and the occurrence of AF revealed by continuous ECG monitoring in a cohort of patients with CS.MethodsSingle-center prospective cohort study. Seventy-two patients with CS with insertable cardiac monitors (ICM) underwent transthoracic echocardiography (TTE). TTE was focused on LA and LV function, including both standard and longitudinal strain-derived parameters. All detected AF episodes lasting more than 2 min were considered.ResultsContinuous ECG monitoring revealed subclinical AF in 23 patients (32%) at an average of 6.5 months after ICM implantation. Many echocardiographic parameters, indicating LA volume and LV systolic/diastolic function, were significantly associated with the occurrence of AF, suggesting the worst atrial function in the AF group. Furthermore, multivariable regression analysis revealed that peak atrial contraction strain and left ventricular strain were independently associated with AF (adjusted OR = 0.72, CI 95% 0.48–0.90, p = 0.005, and adjusted OR = 0.69, CI 95% 0.46–0.95, p = 0.041, respectively).ConclusionIn patients with CS, LA and LV strain analysis add predictive value for the occurrence of AF over clinical and morpho-functional echocardiographic parameters. Impaired booster pump strain and LV longitudinal strain are strong and independent predictors of AF.
Feasibility and Safety of Drug-Coated Balloon-Only Angioplasty for De Novo Ostial Lesions of the Left Anterior Descending Artery: Two-Center Retrospective Study
Authors: Li Chuang , Ding Xuebo , Wang Lefeng , Li Kuibao , Yang Xinchun , Liu Liping , Xu Li 
Published: April 2022

IntroductionThere is limited evidence regarding the effectiveness of drug-coated balloon (DCB) angioplasty in the percutaneous treatment of complex de novo ostial coronary lesions. This study primarily aimed to explore the feasibility and safety of this innovative approach for ostial lesions in the left anterior descending artery (LAD).MethodsPatients treated with paclitaxel DCB or second-generation drug-eluting stents (DES) were retrospectively enrolled from two large centers. The primary endpoints were major adverse cardiovascular events (MACE), including cardiovascular death, target lesion revascularization (TLR), target vessel revascularization, and recurrent myocardial infarction related to target artery occlusion. Cox regression analysis was used to identify risk factors for MACE, and propensity score matching was performed to minimize selection bias.ResultsA total of 388 patients were included; among them, 52 were treated with paclitaxel DCB, and 336 with DES for ostial LAD lesions. Using propensity score matching, 49 patients were treated with DCB-only and 49 with the DES strategy. The average follow-up time was 12 months; subsequently, a non-significant decrease in MACE rate was observed in the DCB-only angioplasty treatment group (MACE: 6 vs. 6%, p = 1.0; TLR: 2 vs. 4%, p = 0.56). Cox regression analysis indicated that DCB-only angioplasty was not an independent risk factor for adverse events after adjusting for confounding risk factors (hazard ratio: 1.713, p = 0.43).ConclusionThe use of the DCB-only approach is an innovative and optional strategy in the treatment of isolated ostial LAD disease. A further randomized trial is of necessity to confirm the feasibility and safety of drug-coated balloon-only angioplasty for LAD de novo ostial lesions.
Unification of the Mechanical Model and Parameter Analysis of the Elastic Foundation Beam of Pipe-Roof
Authors: Xu Xiaojing , Song Zhanping , Li Hui , Tian Xiaoxu , Zhou Guannan 
Published: April 2022

Based on the existing model of pipe-roof considering the arching effect, combined with the mechanical model of pipe-roof when the tunnel is excavated to the end, a unified mechanical model of the elastic foundation beam for pipe-roof is established. Deflection and internal force calculation formulas of the model were derived. Combined with actual engineering cases, the model was compared and analyzed, and the parameters affecting the pipe-roof were analyzed by taking the unified model as an example. The results show that the established unified elastic foundation beam model can better represent the actual stress state of a pipe-roof and the model has strong applicability. The stress state of the pipe-roof at the end of excavation can be calculated by changing the boundary conditions of the model. When the diameter of the steel pipe is 108–114 mm, the supporting effect of the pipe-roof is similar. When Ec (elastic modulus of converging) &gt; 40.0Eg (elastic modulus of ground rock), the excavation footage and the diameter of the steel pipe have little effect on the deflection and bending moment of the pipe-roof. Therefore, increasing the elastic modulus of the reinforced area is the most effective method to reduce the deflection and internal force of the pipe-roof. The longer the residual length of the pipe-roof in surrounding rock, the safer the tunnel will be during excavation. The economically reasonable value of the residual length of the pipe-roof in surrounding rock is 2–3 m.
Cenozoic Cichlids of Africa (Cichlidae: Pseudocrenilabrinae) With the Description of a New Species From the Oligocene of Somalia
Authors: Murray Alison M. 
Published: April 2022

Cichlid fishes are an important component of the African freshwater ecosystem and the evolution of this group has captured the interest of ichthyologists for many decades. The distribution of cichlid fishes in both Africa and South America, and the apparent reciprocal monophyly of the cichlids in the two areas, has led to differences in opinion as to whether their modern distribution reflects a dispersal event from Africa to South America or was the result of a vicariant event–the break-up of the two continental masses in the mid-Cretaceous. If the vicariant event were the cause of the modern distribution, this would indicate an age for the family of at least 120 million years; however, the fossil record indicates a younger, Cenozoic, age for the family. The known fossil taxa in Africa often are difficult to assign to extant tribes which creates inaccuracies when they are used to date lineages within Cichlidae. The difficulty of assigning fossil cichlids to modern lineages is caused by most of these lineages being recognized based on DNA or soft-tissue characters, which are generally not preserved in fossils. This hampers our understanding of the early history of the family. Despite this, new African cichlid fossils that have been described in the past two decades and a new technique to determine relationships of these fossils are beginning to elucidate the early history of the family in Africa. Here the Palaeogene (Eocene and Oligocene) cichlids of Africa are reviewed and a new taxon from the Oligocene of Somalia, Somalichromis hadrocephalus gen. et sp. nov. is described.
Vertical Sand Flux Density and Grain-Size Distributions for Wind-Blown Sand Over a Gobi Surface in Milan, Southern Xinjiang, China
Authors: Tan Lihai , Zhang Kai , Wang Hongtao , An Zhishan , Wang Tao 
Published: April 2022

Vertical sand flux density and grain-size distributions of wind-blown sand over gobi are an essential way for examining the complex grain-bed collisions over gobi surfaces and then understanding aeolian saltation dynamics. However, compared with sand surfaces, relatively few studies have reported regarding how sand flux density and grain size vary with elevation for wind-blown sand over gobi, especially in a field scale. Here, vertical sand flux density and grain-size distributions for wind-blown sand over a typical gobi surface during three transport events were revealed. The results show that the sand flux density exponentially decreased with elevation, which is different from the previous wind tunnel studies, and 99% of the sand transport amount was concentrated in the near-surface layer of 0.6 m. The mean grain size (Dm) first increased with elevation until an inflection in grain-size trends occurred at 0.175 m or 0.285 m above the ground, and then Dm decreased with height, which is significantly different from the vertical grain-size profile patterns of sand surfaces. The grain-bed collision process of medium sand over the gobi surface caused the increase of the mean grain size with height. Sorting was dominated by moderately sorted, skewness by symmetrical or fine skewed, and kurtosis by mesokurtic. The results of this study are significant for future numerical modeling studies of aeolian saltation over rough surfaces on Earth and even on Mars.
Carbon Emissions With Forest Cover Change and Wood Harvest in the Dry Temperate Region of Pakistan Between 1908 and 2015
Authors: Ahmad Adnan , Ahmad Shahid , Nabi Ghulam , Zeb Alam , Rajpar Muhammad Nawaz , Ullah Sami , Khalid Faisal , Rahman Mujibur , Liu Qijing , Zu Kuiling , Guo Xinle , Wanghe Kunyuan 
Published: April 2022

Regional carbon emissions related to forest cover change (FCC) and wood harvest (WH) are critical for the accurate estimates of global carbon balance over an extended time period. Using remote sensing and inventory data, this study provides a comprehensive record of FCC, WH, and their integrated carbon emissions between 1908 and 2015 in the dry temperate regions of Pakistan. Results demonstrate a significant decline in forest area (21,034 ha) at an annual rate of 0.56% from 1973 to 2015. The total WH was 24.84 million m3 (0.23 million m3 yr−1) between 1908 and 2015. Deforestation was responsible for a net loss of 1.39 million Mg C (0.018 million Mg C yr−1), while WH-related carbon emissions accounted for 11.29 million Mg C (0.52 million Mg C yr−1). The present results indicate that under the existing FCC and WH harvest scenario, the forests are acting as a net source of 0.29 million Mg C yr−1. Agriculture expansion and the heavy dependency of local communities on the forest’s resources, exclusion of conservation and local communities from forest management, insufficient monitoring, and weak law-enforcement were the striking drivers of FCC, WH, and their related emissions. These findings suggest that to maintain forest carbon and meet the communities’ requirements, counter approaches such as agriculture incentives, agroforestry, trophy hunting, alternative energy sources, and inclusion of conservation and secure community-based management are needed.
Evaluating a Panel of Autoantibodies Against Tumor-Associated Antigens in Human Osteosarcoma
Authors: Luo Manli , Wu Songmei , Ma Yan , Liang Hong , Luo Yage , Gu Wentao , Fan Lijuan , Hao Yang , Li Haiting , Xing Linbo 
Published: April 2022

Background: The aim of this study was to identify a panel of candidate autoantibodies against tumor-associated antigens in the detection of osteosarcoma (OS) so as to provide a theoretical basis for constructing a non-invasive serological diagnosis method in early immunodiagnosis of OS.Methods: The serological proteome analysis (SERPA) approach was used to select candidate anti-TAA autoantibodies. Then, indirect enzyme-linked immunosorbent assay (ELISA) was used to verify the expression levels of eight candidate autoantibodies in the serum of 51 OS cases, 28 osteochondroma (OC), and 51 normal human sera (NHS). The rank-sum test was used to compare the content of eight autoantibodies in the sera of three groups. The diagnostic value of each indicator for OS was analyzed by an ROC curve. Differential autoantibodies between OS and NHS were screened. Then, a binary logistic regression model was used to establish a prediction logistical regression model.Results: Through ELISA, the expression levels of seven autoantibodies (ENO1, GAPDH, HSP27, HSP60, PDLIM1, STMN1, and TPI1) in OS patients were identified higher than those in healthy patients (p &lt; 0.05). By establishing a binary logistic regression predictive model, the optimal panel including three anti-TAAs (ENO1, GAPDH, and TPI1) autoantibodies was screened out. The sensitivity, specificity, Youden index, accuracy, and AUC of diagnosis of OS were 70.59%, 86.27%, 0.5686, 78.43%, and 0.798, respectively.Conclusion: The results proved that through establishing a predictive model, an optimal panel of autoantibodies could help detect OS from OC or NHS at an early stage, which could be used as a promising and powerful tool in clinical practice.
Identification of Neoantigens and Construction of Immune Subtypes in Prostate Adenocarcinoma
Authors: Gao Yukui , Wang Guixin , Chen Yanzhuo , Zhang Mingpeng , Gao Wenlong , Shang Zhiqun , Niu Yuanjie 
Published: April 2022

Background: Messenger ribonucleic acid (mRNA) vaccine has been considered as a potential therapeutic strategy and the next research hotspot, but their efficacy against prostate adenocarcinoma (PRAD) remains undefined. This study aimed to find potential antigens of PRAD for mRNA vaccine development and identify suitable patients for vaccination through immunophenotyping.Methods: Gene expression profiles and clinical information were obtained from TCGA and ICGC. GEPIA2 was used to calculate the prognostic index of the selected antigens. The genetic alterations were compared on cBioPortal and the correlation between potential antigen and immune infiltrating cells was explored by TIMER. ConsensusClusterPlus was used to construct a consistency matrix, and identify the immune subtypes. Graph learning-based dimensional reduction was performed to depict immune landscape. Boruta algorithm and LASSO logistic analysis were used to screen PRAD patients who may benefit from mRNA vaccine.Results: Seven potential tumor antigens selected were signiﬁcantly positively associated with poor prognosis and the antigen-presenting immune cells (APCs) in PRAD, including ADA, FYN, HDC, NFKBIZ, RASSF4, SLC6A3, and UPP1. Five immune subtypes of PRAD were identified by differential molecular, cellular, and clinical characteristics in both cohorts. C3 and C5 had immune “hot” and immunosuppressive phenotype, On the contrary, C1&amp;C2 had immune “cold” phenotype. Finally, the immune landscape characterization showed the immune heterogeneity among patients with PRAD.Conclusions: ADA, FYN, HDC, NFKBIZ, RASSF4, SLC6A3, and UPP1 are potential antigens for mRNA vaccine development against PRAD, and patients in type C1 and C2 are suitable for vaccination.