bluuebunny/crossref_metadata_2025_split huggingface dataset:
The development of a droplet digital PCR for accurate detection of SARS-CoV- 2 by simultaneous determination of dual gene targets
Authors: Zhang Juanmei , Han Jingru , Liang Yixuan , Bai Chunyang , Liu Wenhua , Wang Xiaoyan , Liao Ziqian , Guo Jianghao , Zhu Qingguo , Liu Hongliang , Bao Dengke , Zhang Yijie 
Published: November 2022

Abstract

Real-time fluorescent quantitative PCR is considered as the gold standard for diagnosing COVID-19 worldwide now. But RT-PCR which could yield semi-quantitative results only, and the sensitivity of this method is usually not sufficient to discriminate patients in early stages of infection or with a very low viral load. So, the false-negative phenomenon often brings difficulties to epidemic prevention and diagnosis. More accurate and robust determine method are required for accurate SARS-CoV-2 diagnosis. To offer an attractive platform for SARS-CoV-2 RNA quantification, we report a droplet digital PCR technique for efficient, accurate, and quantitative detection of SARS-CoV-2 RNA. Two pairs of primer and two double-quenched probes targeting the region of ORF1ab and N protein coding genes of SARS-CoV-2 were designed to develop ddPCR assay. The sensitivity, specificity, repeatability, and reproducibility were tested by standard cDNAs and clinical specimens. First, we found the optimal annealing temperature was 53.6℃, the optimal primer concentration was 500 nM per reaction. When both probes of ORF1ab and N protein are applied simultaneously, their concentrations are100 nM and 50 nM, respectively. The optimal concentration of SARS-CoV-2 standard cDNA ranged from 1×103 to 1×104 copies/µL. And under this condition, the trend line for the measured concentration is Y = 0.9978x-0.4435. Further, the ddPCR were approximately 10 folds greater sensitivity than RT-qPCR assay. The established ddPCR assay with both probes showed to be a highly effective, accurate and reliable method for the sensitive detection of SARS-CoV-2. And the ddPCR method is the most potential and valuable method for clinical detection of SARS-CoV-2 virus.
Design and properties of alginate/gelatin/cellulose nanocrystals interpenetrating polymer network composite hydrogels based on in situ cross-linking
Authors: Li Zhengyue , Liao Yuqing , Li Dongze , Wang Hongcai , Sun Xiuying , Chen Xiuqiong , Yan Huiqiong , Lin Qiang 
Published: November 2022

Abstract
Alginate (Alg) hydrogels have attracted extensive attention in the biomedical field due to their biocompatibility. However, single Alg hydrogels exhibit weak mechanical strength, poor stability and cell adhesion, which severely restricts their biomedical application. For this reason, we designed alginate/gelatin/cellulose nanocrystals (Alg/G/CNCs) composite hydrogels by combining interpenetrating network (IPN) technology, cellulose nanocrystals (CNCs) reinforcement and in situ cross-linking method to improve the functional defects of Alg hydrogels. The structure and properties of the resultant Alg/G/CNCs composite hydrogels were comprehensively evaluated by FT-IR, TGA, XRD, swelling and degradability measurements, and cytocompatibility experiments. Alg/G/CNCs composite hydrogels with regular three-dimensional porous network (3D) structures was successfully fabricated through the ionic cross-linking of alginate and the covalent cross-linking of gelatin, followed by the reinforcement of colloidal cellulose nanocrystals (CNCs) that were prepared by sulfuric acid hydrolysis of microcrystalline cellulose (MCC). The addition of CNCs could generate interaction force with the polymer in the IPN matrix, which was able to regulate the physicochemical properties of the composite hydrogel to a certain extent. Moreover, with the increase of gelatin (G) content, the compressive strength of Alg/G/CNCs composite hydrogels gradually increased, while the swelling property decreased gradually. Meanwhile, Alg/G/CNCs composite hydrogels exhibited good cell adhesion, proliferation and differentiation properties. In particular, Alg/0.5G/CNCs composite hydrogels displayed the best cell proliferation effect, while Alg/2G/CNCs composite hydrogels revealed the most significant cell differentiation effect. Therefore, Alg/G/CNCs composite hydrogels could exhibit good mechanical properties and biocompatibility, which possessed great application potential in the field of tissue engineering.
Analysis of Clinical Efficacy and Quality of Life of Braun Anastomosis in Gastrointestinal Reconstruction in Totally Laparoscopic Distal Gastrectomy: A Single-center Retrospective Study
Authors: Fu Yayan , Ren Jun , Ma Yue , Zhou Jiajie , Shao Wenzhe , Sun Guowei , Sun Qiannan , Wang Daorong 
Published: November 2022

Abstract
Objective
This study aimed to compare the clinical efficacy and quality of life of B-IIB( Billroth-II with Braun anastomosis) and B-II (Billroth-II anastomosis) in the alimentary tract reconstruction postoperative totally laparoscopic distal gastrectomy (TLDG) for gastric cancer.
Methods
From February 2016 to January 2022, 158 patients underwent totally laparoscopic distal gastrectomy and D2 lymphadenectomy in Northern Jiangsu People’s Hospital, with Billroth-II with Braun anastomosis for 93 patients and Billroth-II anastomosis for 65 patients. The patients’s data were collected prospectivly and reviewed retrospectively.
Results
In this study, the post-op hospital stay for B-IIB group were shorter than for B-II group (12.70 ± 3.08 days in the B-IIB group versus 14.12 ± 4.90 days in the B-II group, p < 0.05) and the first post-op anal wind time for the B-IIB group were shorter than for B-II group (3.49 ± 1.02 days versus 4.08 ± 1.85 days, p < 0.05). Two groups did differ significantly in hemoglobin on postoperative 3 months, albumin at 3 months after operation and serum sodium on postoperative 3 days and 3 months (p < 0.05), and the B-IIB had an advantage; the incidence of complications of Clavien-Dindo grade II or higher in the B-IIB group and B-II were 10.75% and 29.23%, respectively. There being a statistical difference between the two groups. The B-IIB group and the B-II group both had different degrees of weight loss at 3 months after operation compared with preoperative weight. The weight of Billroth-II combined with Braun reconstruction group was 4.04 ± 1.33 kg, which was less than 8.08 ± 1.47kg of Billroth-II reconstruction group.The difference was statistically significant (p < 0.05). According to the PGSAS ( Postgastrectomy Syndrome Assessment Scale), the score of the Billroth-II with Braun reconstruction group is lower than that of the Billroth-II reconstruction group for esophageal reflux gastritis, dyspepsia and dumping syndrome group (1.84 ± 0.92 VS 2.15 ± 0.85, P = 0.031; 1.86 ± 1.10 VS 2.22 ± 0.91, P = 0.034; 1.98 ± 1.06 VS 2.32 ± 0.94, P = 0.037, respectively).
Conclusion
Totally laparoscopic distal gastrectomy with Billroth-II Braun reconstruction is a safe and technically feasible method for gastric cancer patients, which can reduce the incidence of postoperative reflux esophagitis and dumping syndrome. Compared with Billroth-II reconstruction,it has advantages in maintaining postoperative nutritional status and electrolyte balance and improving quality of life.
Research on the construction and functions of intelligent food safety supervision systems
Authors: Wang Guanzhao , Liu Shijia , Cao Jing , Wang Yajie , Ren Pingping , Wu Yongning , He Qinghua 
Published: November 2022

Abstract
This study investigated the existing problems and the optimal solutions in the construction of intelligent food safety supervision systems globally. Through browsing manually and searching by keywords about systems using the Internet as a medium, a total of 35 intelligent systems were collected and analyzed from the aspects of the servers, system performance, user groups, client construction, main functions, and characterized functions. The results showed that the current situation was a lack of unified data standards, the food safety risk early-warning model, sufficient system functional design and food safety professionals' participation, and systematic rules and regulations for system operation. Therefore, improvement measures including developing unified data standards, strengthening the cooperation and sharing of data resources, improving the information-sharing mechanism, building the multi-step dynamic risk early-warning models, and optimizing and improving the subfunctions and characteristic functions should be taken in the future.
Dietary phytoestrogen, diosgenin interrupts metabolism, physiology and reproduction of the Swiss albino mice: possible mode of action as an emerging environmental contaminant, endocrine disruptor and reproductive toxicant
Authors: Khushboo Maurya , Sanjeev Sanasam , Murthy Meesala Krishna , Sunitadevi Maibam , Dinata Roy , Bhanushree Baishya , Bidanchi Rema Momin , Nisa Nisekhoto , Lalrinzuali Sailo , Manikandan Bose , Saeed Ahmed-Laskar , Abinash Giri , Pori Buragohain , Arati Chettri , Roy Vikas Kumar , Gurusubramanian Guruswami 
Published: November 2022

Abstract
Dietary phytoestrogens are the main sources of environmental contamination because of their estrogen-mimic and endocrine-disrupting effects and pose a threat to microbial, soil, plant and animal health. Diosgenin, a phytosteroid saponin, is being used as nutraceuticals, dietary supplements, contraceptive, and in the hormone replacement therapy against numerous diseases and disorders. Due to the lack of research on the safety and probable adverse side effects of diosgenin, this work aimed to evaluate the endocrine disrupting and reproductive toxicity of diosgenin in the albino mice with respect to acute toxicity (OECD-423), repeated dose 90 days oral toxicity (OECD-468), and F1 extended one generation reproductive toxicity (OECD-443) studies. Diosgenin is a slightly toxic compound and LD50 for male and female mice is 546.26 and 538.72 mg/kg, respectively. Chronic exposure of diosgenin (10, 50, 100, and 200 mg/kg) generated oxidative stress, depleted antioxidant enzymes, disturbed homeostasis of the reproductive hormones, and interrupted steroidogenesis, germ cell apoptosis, gametogenesis, sperm quality, estrous cycle and reproductive performance in the F0 and F1 offspring. Long-term oral exposure of diosgenin to the mice interrupted the endocrine and reproductive functions, and triggered the transgenerational reproductive toxic effects in F0 and F1 offspring, validating the possible mode of action as an emerging environmental contaminant, endocrine disruptor and reproductive toxicant in Swiss albino mice.
Design of Interactive Music Teaching System for Wireless Communication Application by Convolutional Neural Network Optimization and Edge-Cloud Computing
Authors: Zeng Nani 
Published: November 2022

Abstract
This study aims to improve the effectiveness of online music teaching and optimize the current interactive system of online teaching. Firstly, the basic connotation of Convolutional Neural Network (CNN), edge-cloud computing and their application principles are discussed. Then, the basic principles of wireless communication and its comprehensive optimization methods are discussed. Finally, the Softmax Convolutional Neural Network-Long Term Evolution (SCNN-LTE) model based on CNN to optimize wireless communication technology is designed. Model skills are comprehensively assessed. The results show that, compared with other models, the comprehensive performance of the designed CNN model has been greatly improved. The evaluation found that the recall value of the Softmax Convolutional Neural Network (SCNN) model is around 0.9-1.0, and the precision value is around 0.8–0.9. Additionally, CNN models are applied to wireless communication technologies for performance evaluation. The accuracy of the SCNN-LTE model is generally between 0.7 and 0.9. The designed model not only optimizes the CNN model to a certain extent but also deeply optimizes the wireless communication technology. Therefore, the model can be better applied to the online music teaching interactive system, providing important technical support for its effect optimization. This study not only provides a technical reference for the optimization of wireless communication technology but also contributes to the performance enhancement of the online music teaching interactive system.
Curriculum Design of Sports Humanities and Sociology Based on Intelligent Edge Cloud Computing and Artificial Intelligence
Authors: Zhang Liangyu , Zhao Jinkun 
Published: November 2022

Abstract
The course of sports humanities and sociology occupies an important position in the contemporary education system. However, in the classroom teaching process, the current teaching of sports humanities and sociology is far from the expected effect, which is not conducive to the play of the value and role of the curriculum. In this context, it is very necessary to innovate and explore the teaching mode of sports humanities and sociology courses. Under the artificial intelligence wireless network environment, the professional courses of sports humanities and sociology have been researched and set up, and a reconstruction decision algorithm is proposed. In the course setting, the intelligent edge cloud computing theory designed the information management system for massive course data. When the system is deployed, edge nodes are introduced into each data collection area. The system consists of data collection, transmission, and query and analysis modules. Firstly, the network parameters that need to be changed are obtained by analyzing the factors that affect the performance, and the data are reconstructed and deployed. These changes are mapped into the actual network structure. This not only ensures that the system can adapt to the dynamically changing environment but also saves time of system operation. Two results are obtained: on the one hand, the artificial neural network is good at processing complex multi-domain information; on the other hand, artificial intelligence introduces a learning mechanism into reconstruction decision-making, which ensures the correctness and optimization of reconstruction decisions. The course data management system based on intelligent edge computing can meet the storage and query requirements of millions of data items. Based on this algorithm, the sports humanities and sociology courses are set up to maintain a balanced state that ensures the course runs well. Ultimately, sports humanities and sociology courses can better serve teachers and students and then serve social progress.
Managing Chronic Pain in Cancer Survivorship: Communication Challenges and Opportunities as Described by Cancer Survivors
Authors: O'Regan Amy , Fish Laura J , Makarushka Christina , Somers Tamara , Jones Katie Fitzgerald , Merlin Jessica , Dinan Michaela , Oeffinger Kevin , Check Devon K 
Published: November 2022

Abstract
Purpose

Many cancer survivors experience chronic pain after completing curative-intent treatment. Based on available data, chronic pain may be undertreated in this context; however, little is known about cancer survivors’ experiences with clinical management of chronic pain. The purpose of this study was to better understand cancer survivors’ pain management experiences after curative-intent treatment.

Methods

We conducted 13 semi-structured interviews with a convenience sample of cancer survivors who had completed treatment for stage I-III breast, head/neck, lung or colorectal cancer. We used a thematic approach to qualitative data analysis.

Results

Participants described that chronic pain often goes unrecognized by their providers, potentially due to limitations in how pain is assessed clinically and the tendency of both cancer survivors and some providers to minimize or invalidate the pain experience. To improve communication, participants suggested that providers ask more open-ended questions about their pain, help them to establish functional goals, and provide patients with options for pain management.

Conclusions

This study demonstrates the importance of provider-initiated communication around pain management for cancer survivors to make them feel more supported in their care.

Implications for Cancer Survivors

Communication and shared decision-making interventions may improve cancer survivor-provider communication around chronic pain management, addressing an important gap in survivorship care.
Urban/Rural Spatial Price Discrimination with Online Competition
Authors: Guo Wen-Chung , Lai Fu-Chuan 
Published: November 2022

Abstract

We contribute to the previous offline spatial price discrimination by adding an online entrant that results in partially equalized prices, and the urban (rural) segments are served by the offline (online) firms. Online competition induces the offline firms to move closer to the market center, which causes the equilibrium locations to no longer be the social optimum due to the online price distortion. The greater the online advantage is, the higher the online price, the less dispersed the offline locations, and the lower the offline prices will be in urban areas. Finally, zoning policies and two extensions of online price discrimination and multiple offline firms with free entry are offered.
JEL Classification Numbers. R30, L13
A Novel Compact, Tunable Positive and Negative Impedance Simulator and Impedance Multiplier
Authors: absi Muneer Al 
Published: November 2022

Abstract
This paper presents a novel CMOS tunable positive and negative active inductor simulator (AIs), positive capacitance and resistance multiplier and negative capacitance and resistance simulator. The proposed designs use only one second-generation voltage-mode conveyor (VCII) and three passive elements. Applications to the proposed design in the design of different types of tunable filters are also presented. The functionality of the design is confirmed using Tanner Tspice in 0.18µm TSMC CMOS technology.
Graphitized lignin-based activated carbon fibers and their application in supercapacitors
Authors: You Xiangyu , Wang Lingjie , Zhou Linfei , Uraki Yasumitsu , Gele Aori , Kang Jia , Li Xinhong , Zhang Hui Jie 
Published: November 2022

Abstract
As an abundant bioresource of carbon, lignin has the potential to form carbon fibers (CFs), which can then be used as electrode materials in supercapacitors. However, most studies have only focused on the morphology of CFs, such as the specific surface area and pore-size distribution, while ignoring the influence of the carbon structure in CFs. In this study, Ni(acac)2 was used as a graphitization catalyst in CFs preparation and to further modify the morphology of the fibers. Meanwhile, the addition of Ni(acac)2 remarkably shortened the thermostabilization period from 76 h to 2.25 h, which significantly reduced the energy consumption. The resulting lignin-based activated carbon fibers (LACFs) with graphitized structures presented a controllable pore-size distribution and large surface areas up to 582.7 m2 g− 1. These LACFs were then used as electrode materials in supercapacitors, and the assembled devices displayed high energy and power densities of up to 22.1 Wh kg− 1 and 239 kW kg− 1, respectively, using organic electrolytes.
Tetrahedrite-(Ni), Cu6(Cu4Ni2)Sb4S13, the first nickel member of tetrahedrite group mineral from Luobusa chromite deposits, Tibet, China
Authors: Wang Yanjuan , Chen Rujun , Gu Xiangping , Nestola Fabrizio , Hou Zengqian , Yang Zhusen , Dong Guochen , Guo Hu , Qu Kai 
Published: November 2022

Abstract
Tetrahedrite-(Ni) (IMA2021-031), ideally Cu6(Cu4Ni2)Sb4S13, is the first natural Ni-member of tetrahedrite group mineral found in Luobusa chromite deposit, Tibet, China. The new species occurs as anhedral grains 2 to 20 μm in size, associated with gersdorffite, vaesite, and chalcostibite, which are disseminated in a matrix of dolomite, magnesite, quartz, Cr-rich mica, and Cr-bearing clinochlore. Tetrahedrite-(Ni) is black in color with a reddish-black streak and metallic luster. It is brittle with uneven fractures and has a calculated density of 5.073 g·cm–3. The mean values of 9 electron microprobe analyses (wt%) are Cu 39.83, Ni 5.67, Fe 1.45, Sb 21.69, As 5.45, S 25.39, total 99.48, and the empirical formula calculated on the basis of cation = 16 apfu is M(2)Cu6.00M(1)[Cu4.03(Ni1.55Fe0.42)Σ1.97]Σ6.00X(3)(Sb2.85As1.16)Σ4.01S12.67. Tetrahedrite-(Ni) is cubic, with space group I43m, a = 10.3478(4) Å, V = 1108.00(14) Å3, and Z = 2. Its crystal structure has been solved by X-ray single-crystal diffraction on the basis of 188 independent reflections, with a final R1 = 0.0327. Tetrahedrite-(Ni) is isostructural with tetrahedrite group minerals. It represents the first natural tetrahedrite-group mineral with a Ni-dominated charge-compensating constituent. Tetrahedrite-(Ni) may be the product of late-serpentinization at moderately high-temperature conditions around 350 °C. In this case, tetrahedrite-(Ni) and its mineral paragenesis record an entire geological process of nickel enrichment, migration, activation, precipitation, and alteration from deep mantle to shallow crust.
Penerapan Konsep Continuous Review (Q,r) Pada Model Economic Order Quantity (EOQ) Uutuk Mengoptimalkan Persediaan Bahan Baku Minuman
Authors: Romariardi Adi Wahyu , Mustafid Mustafid , Suryono Suryono 
Published: November 2022

The challenge in controlling the supply of beverage ingredients is strongly influenced by the stable condition of supply, prices and differences in the shelf life of the beverage ingredients themselves. The high cost of beverage ingredients such as milk, flavoring powder and the difficulty of finding supplies of certain beverage ingredients, all of the increase in prices and difficulties will certainly affect operations. There are three choices, either increase the price, reduce the profit, or reduce the portion size. All these choices will not be easy to decide. This study aims to build an information system by applying the concept of continuous review (Q,r) on the economic order quantity (EOQ) model to optimize the supply of beverage ingredients. With the application of the concept of continuous review (Q, r) in the economic order quantity (EOQ) model on robusta coffee beans produces the order quantity is 521.15 grams each time an order is at the inventory level at 746 grams, palm sugar produces the order quantity is 1,430.71 grams every time you order at a stock level at 1124 grams, UHT milk produces the order quantity is 1,188.4 ml each time you order at a stock level at 7462 ml, and sweetened condensed milk produces an order amount of 802,6 grams each time you order at a stock level of 758 grams.
Towards explicit discrete holography: Aperiodic spin chains from hyperbolic tilings
Authors: Basteiro Pablo , Di Giulio Giuseppe , Erdmenger Johanna , Karl Jonathan , Meyer Rene , Xian Zhuo-Yu 
Published: November 2022

We propose a new example of discrete holography that provides a new
step towards establishing the AdS/CFT duality for discrete spaces. A
class of boundary Hamiltonians is obtained in a natural way from regular
tilings of the hyperbolic Poincaré disk, via an inflation rule that
allows to construct the tiling using concentric layers of tiles. The
models in this class are aperiodic spin chains, whose sequences of
couplings are obtained from the bulk inflation rule. We explicitly
choose the aperiodic XXZ spin chain with spin 1/2 degrees of freedom as
an example. The properties of this model are studied by using strong
disorder renormalization group techniques, which provide a tensor
network construction for the ground state of this spin chain. This can
be regarded as discrete bulk reconstruction. Moreover we compute the
entanglement entropy in this setup in two different ways: a
discretization of the Ryu-Takayanagi formula and a generalization of the
standard computation for the boundary aperiodic Hamiltonian. For both
approaches, a logarithmic growth of the entanglement entropy in the
subsystem size is identified. The coefficients, i.e. the effective
central charges, depend on the bulk discretization parameters in both
cases, albeit in a different way.