bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postoperative hemorrhage following pancreatic injury: Risk factors and clinical outcomes
Authors: Wang Kai , Li Kaiwei , Deng Yunxuan , Wang Xinyu , Yang Chao , Chu Chengnan , Li Weiqin , Li Jieshou , Ding Weiwei 
Published: October 2022

AbstractBackgroundPostoperative hemorrhage (POH) is a severe adverse event following pancreatic injury. The present study aimed to investigate the risk factors and outcomes of POH after pancreatic injury.MethodsAll patients with a confirmed diagnosis of pancreatic injury who underwent surgical intervention between January 2010 and December 2018 were identified and extracted from the trauma database. Logistic regression was performed to identify the risk factors for POH and specific outcomes.ResultsA total of 88 cases that underwent surgical intervention were analyzed, and POH occurred in 31 (35.23%) patients. After multivariable analysis, independent predictors of POH were intra‐abdominal abscess (IAA) (p = .002), intestinal fistula (p = .008), shock on admission (p = .003), absence of abdominal suction drainage (p = .005), and higher body mass index (BMI) (p = .005). In addition, patients with POH after pancreatic injury also showed more complications, prolonged hospital and ICU durations, and a significantly higher mortality rate (p = .004).ConclusionsPatients with IAA, intestinal fistula, shock on admission, absence of abdominal suction drainage, and higher BMI score were associated with POH after pancreatic injury. Moreover, POH was associated with a poor prognosis.Clinical Trial RegisterThe study was registered on ClinicalTrials.gov (Unique identifier: NCT03681041).
MRI of Lymphedema
Authors: Salehi Betsa Parsai , Sibley Robert Carson , Friedman Rosie , Kim Geunwon , Singhal Dhruv , Loening Andreas Markus , Tsai Leo L. 
Published: October 2022

Lymphedema is a devastating disease that has no cure. Management of lymphedema has evolved rapidly over the past two decades with the advent of surgeries that can ameliorate symptoms. MRI has played an increasingly important role in the diagnosis and evaluation of lymphedema, as it provides high spatial resolution of the distribution and severity of soft tissue edema, characterizes diseased lymphatic channels, and assesses secondary effects such as fat hypertrophy. Many different MR techniques have been developed for the evaluation of lymphedema, and the modality can be tailored to suit the needs of a lymphatic clinic. In this review article we provide an overview of lymphedema, current management options, and the current role of MRI in lymphedema diagnosis and management.Evidence Level5Technical EfficacyStage 5
Development and characterization of a new monoclonal antibody against SARS‐CoV‐2 NSP12 (RdRp)
Authors: Meng Wen , Guo Siying , Cao Simon , Shuda Masahiro , Robinson‐McCarthy Lindsey R. , McCarthy Kevin R. , Shuda Yoko , Paniz Mondolfi Alberto E. , Bryce Clare , Grimes Zachary , Sordillo Emilia M. , Cordon‐Cardo Carlos , Li Pengfei , Zhang Hu , Perlman Stanley , Guo Haitao , Gao Shou‐Jiang , Chang Yuan , Moore Patrick S. 
Published: October 2022

AbstractSARS‐CoV‐2 NSP12, the viral RNA‐dependent RNA polymerase (RdRp), is required for viral replication and is a therapeutic target to treat COVID‐19. To facilitate research on SARS‐CoV‐2 NSP12 protein, we developed a rat monoclonal antibody (CM12.1) against the NSP12 N‐terminus that can facilitate functional studies. Immunoblotting and immunofluorescence assay (IFA) confirmed the specific detection of NSP12 protein by this antibody for cells overexpressing the protein. Although NSP12 is generated from the ORF1ab polyprotein, IFA of human autopsy COVID‐19 lung samples revealed NSP12 expression in only a small fraction of lung cells including goblet, club‐like, vascular endothelial cells, and a range of immune cells, despite wide‐spread tissue expression of spike protein antigen. Similar studies using in vitro infection also generated scant protein detection in cells with established virus replication. These results suggest that NSP12 may have diminished steady‐state expression or extensive posttranslation modifications that limit antibody reactivity during SARS‐CoV‐2 replication.
Monkeypox viral detection in semen specimens of confirmed cases: A systematic review and meta‐analysis
Authors: Reda Abdullah , Abdelaal Abdelaziz , Brakat Aml M. , Lashin Basant Ismail , Abouelkheir Moustafa , Abdelazeem Basel , Rodriguez‐Morales Alfonso J. , Sah Ranjit 
Published: October 2022

AbstractThe current literature shows increasing concerns about potential seminal transmission of monkeypox virus (MPXV). Accordingly, we aimed to understand better the potential presence of MPXV in the seminal fluids and others specimens obtained from MPX cases. On June 26, 2022, a systematic search of the literature was conducted to find articles that examine the presence of MPXV in the seminal fluid of confirmed cases. The search was updated once on August 12 and another on October 12, 2022, to include newly published articles. The prevalence of MPXV DNA presence in the seminal fluid and other specimens was pooled in a meta‐analysis (from studies with sample size > 5 to reduce overestimation) and results were presented as effect sizes (ES) and their corresponding 95% confidence intervals (CI). Nine articles were included. Only five studies were eligible for a meta‐analysis, and the pooled prevalence of MPXV DNA in semen specimens was 72.4% (95% CI: 55.7%−84.5%) among 115 patients. The positive rate of MPXV viral polymerase chain reaction (PCR) was higher among skin samples (89%; 95% CI: 78.2%−94.8%; N = 62; studies = 2), followed by anogenital/rectal samples (74.3%; 95% CI: 60.4%−84.5%; N = 54; studies = 2). On the other hand, the positivity rate was lower in nasopharyngeal (62.4%; 95% CI: 20.4%−91.5%; N = 587; studies = 3), urine (21.1%; 95% CI: 4.3%−61.1%; N = 617; studies = 4), and blood/plasma (14.3%; 95% CI: 11.3%−18.1%; N = 609; studies = 3) samples. Besides, MPXV can be detected in semen early from Day 1 and up to 19 days after symptoms onset. Finally, two articles investigated the infectivity of MPXV particles detected in seminal specimens by testing their replication competence. Culturing MPXV was successful in two out of four patients included in these studies. MPXV is highly prevalent in seminal specimens of MPX cases, further corroborating the role of sexual transmission of the disease. However, further evidence is still needed to shed more light on the replication competence of these particles.
Isolation and characterization of bacteriophage Ib_pec2 against shigatoxigenic Escherichia coli
Authors: Inbaraj Sophia , Angappan M. , Thomas Prasad , Kumar Manish , Irungbam Karuna , Verma Med Ram , Viswas K. Nagaleekar , Abhishek  , Rawat Mayank , Chaudhuri Pallab 
Published: October 2022

AbstractThis study was aimed to isolate and characterize bacteriophage against drug‐resistant, shigatoxigenic Escherichia coli (STEC), one of the zoonotic, food‐borne organisms associated with ruminants, mainly cattle. STEC were isolated (n = 35) from neonatal calves, dairy workers, and the surrounding environment and their antimicrobial resistance pattern was studied. Out of the 35 isolates tested, 17 isolates were found to be multidrug resistant to important antibiotics like ampicillin, amoxicillin‐clavulanate, ciprofloxacin, streptomycin, and tetracycline. Bacteriophage namely Ib_pec2 was isolated against one of the STEC isolates and its morphology, genetic and proteomic characterization was done. Morphological analysis by TEM revealed bacteriophages belonging to myoviridae family. The genetic characterization of g23 gene revealed that the bacteriophage belonged to Tequatrovirus of myoviridae family. Proteomic analysis was able to identify five proteins identical to Tequatrovirus of myoviridae family. One‐step growth curve experiment revealed a latency period of 40 min and a burst size of 893 pfu/bacteria. Temperature and pH ranging from 40°C to 50°C, pH 6–8, respectively. Phage could able to lyse majority of the STEC isolates. STEC are commensal organisms in the gastrointestinal tract of ruminants but are pathogenic in humans. Bacteriophages can be used as alternatives to antibiotics to control bacterial growth in ruminants and prevent its further spillage in the environment.
Development and validation of a liquid chromatography‐tandem mass spectrometry method for CPUL1, a novel antitumor candidate compound, and its application to pharmacokinetic studies
Authors: Lu Yuanyuan , Zhang Yanjun , Yuan Man , Chen Jiaqin , Xu Yifan , Jie Qiong , An Zhilong , Liao Jianmin , Liu Yunxin , Li Jun , Zhu Yubing , Zou Jianjun , Ge Chun , Feng Dong , Wang Ying 
Published: October 2022

An active substance of pyrano[3,2‐a]phenazine, also called CPUL1, is a synthesized phenazine derivative and displays broad‐spectrum anticancer activities. Quantitative assessment of CPUL1 in biological samples has not been well established, hindering pharmaceutical development and application. According to international guidelines, a sensitive and selective liquid chromatography‐tandem mass spectrometry method in negative ion mode was developed and validated for quantification of CPUL1 in human plasma, colorectal cancer cell lines, and rat plasma, whereby linearity and accuracy were demonstrated for the range of 1–1000 ng/ml. The validated liquid chromatography‐tandem mass spectrometry method was successfully employed in pharmacokinetic studies of CPUL1 in vitro and in vivo. Notably, the cellular pharmacokinetic behavior of CPUL1 varies in colorectal cancer cell lines. Regarding the pharmacokinetic processes in vivo, oral absorption was less effective than an injection, with a bioavailability of 23.66%. CPUL1 was linearly eliminated after a single administration; however, it could accumulate in tissues (heart, liver, spleen, lung, and kidney) after multiple injections. In summary, this study established a capable bioanalytical method for CPUL1 and provided exploratory pharmacokinetic data, paving the way for use of this promising derivative in disease models.
Platelets lipidomics study of blood stasis rat model by using liquid chromatography‐tandem mass spectrometry
Authors: Sun Mingqian , Li Ying , Hu Guang , Yang Huizhen , Zhang Guoyuan , Miao Lan , Zhang Ying , Lin Li , Liu Jianxun 
Published: October 2022

This study analyzed a lipidomic profile of platelets from blood stasis rats by liquid chromatography‐tandem mass spectrometry. The blood stasis rat was established by low‐dose continuous subcutaneous injection of adrenaline, and the evaluation indexes included hemorheology and platelet aggregation. Principal component analysis and partial least‐squares discriminant analysis were used to analyze platelet lipidomics, and p‐value < 0.05, fold change > 1.5, and variable importance plot > 2 were used to screen potential biomarkers. Then, the biomarkers were optimized by the receiver operating characteristic curve. Compared with the normal rat, the blood stasis model group's whole blood viscosity and platelet aggregation rate were also significantly increased at different shear rates (p < 0.05). Twenty‐four potential lipid biomarkers showed significant changes in platelets between the two groups. Among them, six long‐chain acylcarnitine components and three sphingosine components showed a consistent downward trend, suggesting that these two kinds of components may play an essential role in the process of platelet aggregation. Liquid chromatography‐tandem mass spectrometry‐based lipidomics studies provide much information to understand the pathology of platelets in blood stasis.
Expression of Glial Cell‐Derived Neurotrophic Factor Receptors Within Nucleus Ambiguus During Rat Development
Authors: Blount Quinton , Hernandez‐Morato Ignacio , Moayedi Yalda , Pitman Michael J. 
Published: October 2022

ObjectiveThe nucleus ambiguus (NAmb) is a column of neurons in the medulla oblongata, involved in bulbar functions. Expression of Glial Cell‐Derived Neurotrophic Factor (GDNF) and its receptors (GDNFR) is observed within the cell bodies during reinnervation following recurrent laryngeal nerve (RLN) injury. Little is known regarding GDNFR expression in the formation of the NAmb and the laryngeal innervation during embryogenesis. Understanding the timing and pattern of GDNFR expression in embryogenesis versus after RLN injury may provide insights into therapeutic targets for regeneration after RLN injury.Study DesignLaboratory experiment.MethodsRat brainstems at E14.5/E16.5/E18.5/E20.5/adult were stained for GDNFR: GFRα‐1/GFRα‐2/GFRα‐3/Ret. Islet1 and choline acetyltransferase were used as cell body markers. Sections were observed using fluorescent microscopy and quantified through manual cell counting.ResultsExpression of GFRα‐1, GFRα‐3, and Ret was identified within the NAmb, hypoglossal, and facial nuclei of the adult medulla. During development, GFRα‐1 immunoreactivity was seen at E20.5. GFRα‐2 expression was not observed at any timepoint. GFRα‐3 expression began at E16.5. Ret expression within nerve fibers in the NAmb were observed beginning at E14.5, but never in the cell bodies.ConclusionEmbryonic GDNFR expression in the NAmb differs from that of the adult after RLN injury. The developing brainstem experienced upregulation at discrete timepoints with signaling sustained through adulthood. In contrast, adult RLN‐transected rats experienced patterns of up and down regulation. GFRα‐1 may contribute to muscle targeting and neuromuscular junction maturation, GFRα‐3 may contribute to both, as well as axon guidance. It is likely that GDNF is functioning via a Ret‐independent pathway.Level of EvidenceNA Laryngoscope, 133:2240–2247, 2023
Abnormal Laryngopharyngeal Sensation in Adductor Laryngeal Dystonia Compared to Healthy Controls
Authors: Young VyVy N. , Kidane Joseph , Gochman Grant E. , Bracken David J. , Ma Yue , Rosen Clark A. 
Published: October 2022

Background/ObjectivesLaryngeal sensory abnormality has been implicated as a component of adductor laryngeal dystonia (AdLD). The study objective was to assess laryngopharyngeal sensation in AdLD utilizing a calibrated, tactile aesthesiometer to deliver differential stimuli to lateral pyriform sinus (LPS), aryepiglottic fold (AEF), and false vocal fold (FVF).MethodsPatients with known Botox‐responsive AdLD underwent sensory testing using a previously‐validated methodology involving calibrated tactile stimuli (6–0, 5–0, 4.5–0, 4–0 nylon monofilaments). Laryngeal adductor reflex (LAR) and participant‐rated perceptual strength of stimulI were evaluated. Responses were compared to normative controls (n = 33). Two‐samples, Mann–Whitney and Fisher exact tests compared mean strength ratings and LAR between AdLD and control groups. Mixed‐effects logistic regression and linear models assessed association of filament size, stimulus site, age, sex, and LD status on LAR and perceptual strength rating respectively.ResultsThirteen AdLD patients (nine women, mean age 60+/−15 years) completed testing. Average LAR response rates were higher amongst all filament sizes in AdLD versus controls at LPS (56.3% vs. 35.7%) and AEF (96.1% vs. 70.2%) with comparable rates at FVF (90.2% vs. 91.7%). AdLD had 3.3 times the odds of observed LAR compared to controls (p = 0.005), but differences in subjective detection of stimuli, perceptual strength ratings, and cough/gag rates were insignificant on multivariate modeling (p > 0.05).ConclusionsThis is the first study to objectively assess laryngopharyngeal sensation in AdLD. Findings demonstrated increased laryngopharyngeal sensation in AdLD compared to controls. The identification of increased laryngeal hypersensitivity in these patients may improve understanding of AdLD pathophysiology and identify future targets for intervention.Level of Evidence2 Laryngoscope, 133:2271–2278, 2023
Virtual planning and 3D‐printed guides for mandibular reconstruction: Factors impacting accuracy
Authors: Annino Donald J. , Sethi Rosh K. , Hansen Elisabeth E. , Horne Sylvia , Dey Tanujit , Rettig Eleni M. , Uppaluri Ravindra , Kass Jason I. , Goguen Laura A. 
Published: October 2022

AbstractObjectivesExamine accuracy and factors impacting accuracy for mandibular reconstruction with virtual surgical planning, 3D printed osteotomy guides and preoperatively bent mandibular reconstruction plate (VSP/3Dprinted‐guide/plate).MethodRetrospective review of osseous‐free‐flap mandibular reconstructions with VSP/3Dprinted‐guide/plate between January 2015 and July 2020 at a single academic medical center.Patient demographics, disease, and treatment variables were extracted. Accuracy was assessed by 3D‐model‐overlay with cephalometric and donor‐bone segment length measurements. Multivariate analyses were performed to determine factors impacting cephalometric accuracy.Results60 cases met criteria: 41 (68%) cancer, 14 (23%) osteoradionecrosis (ORN), 5 (8%) secondary mandibular reconstruction. Thirteen cases (22%) were Brown class III or IV. Thirty‐nine cases (65%) had ≥2 flap bone segments. Average donor‐bone length was 82 mm (SD: 28). 3D‐model‐overlay accuracy demonstrated minimal deviation between planned and actual reconstruction: intercondylar distance = 2.10 mm (SD: 2.2); intergonial distance = 2.23 mm (SD: 1.9); anterior–posterior distance (APD) = 1.76 mm (SD: 1.5); gonial angle (GA) = 3.11 degrees (SD: 2.4). Mean change in donor‐bone segment length inferiorly was 2.67 mm (SD: 2.6) and superiorly 3.27 mm (SD: 3.2). Higher number of donor‐bone segments was associated with decreased accuracy in GA (p = .023) and longer donor‐bone length was associated with decreased accuracy in APD (p = .031).ConclusionTo our knowledge this is the largest series assessing surgical accuracy of VSP/3Dprinted‐guide/plate for osseous‐free‐flap mandibular reconstruction. We demonstrate highly accurate results, with increased number of donor‐bone segments and donor‐bone length associated with decreased accuracy. Our findings further support VSP/3Dprinted‐guide/plate as a reliable and accurate tool for mandibular reconstruction.Level of EvidenceLevel 4.
Laryngeal complications after endotracheal intubation and prone positioning in patients with coronavirus disease 2019
Authors: Aibara Shiori , Okada Masahiro , Tanaka‐Nishikubo Kaori , Asayama Rie , Sato Eriko , Sei Hirofumi , Aoishi Kunihide , Takagi Taro , Teraoka Masato , Mukai Naoki , Konishi Saki , Okita Mitsuo , Ogawa Siro , Annen Suguru , Ohshita Muneaki , Matsumoto Hironori , Murata Satoru , Harima Yutaka , Kikuchi Satoshi , Takeba Jun , Sato Norio , Hato Naohito 
Published: October 2022

AbstractObjectivesLaryngeal complications have been reported after endotracheal intubation and prone positioning in patients with critical coronavirus disease 2019 (COVID‐19), but their association is unclear. In this study, we investigated the rate of laryngeal complications in patients with COVID‐19 compared to an alternative condition (control group).MethodsWe retrospectively analyzed the data of 40 patients who underwent endotracheal intubation for either COVID‐19 or an alternative condition (control group). Data on age, sex, body mass index (BMI), cardiovascular disease (CVD) risk factors, use of prone therapy, duration of endotracheal intubation, and duration from extubation/tracheostomy to laryngeal evaluation were collected from medical records.ResultsThere were no significant differences in BMI, frequency of CVD risk factors, duration of endotracheal intubation, or duration from extubation/tracheostomy to laryngeal evaluation between the two groups. In the COVID‐19 group, all patients adopted the prone position. In comparison, only one patient in the control group adopted the prone position. Significant differences were observed between the two groups regarding the incidence of vocal fold immobility and laryngeal granuloma.ConclusionLaryngeal complications were more common in the COVID‐19 group than in the control group. Prone positioning may be a risk factor for these complications.Level of Evidence4.
Dynamic Covalent Structure for the Design of Recyclable Polyurethane Based on the Diketone Chemistry
Authors: Bai Jing , Shi Zixing 
Published: October 2022

AbstractHerein, the rational design of an enaminone compound is reported which can be easily and conveniently designed and obtained via the reaction of amine and diketone. The dynamic enaminoneamide structure is formed via the reaction between isocyanate and enaminone in fabricating a novel dynamic crosslinked polyurethane. The new kind of polyurethane can be efficiently recycled via the shifting between crosslinked structure and oligomer for the reversible dissociation of the enaminoneamide structure. Besides remolding itself via the thermal treatment, oligomers liberated from recycled‐polyurethane can directly take part in constructing a new polyurethane without further purification. Therefore, the recyclability of this polyurethane shows high‐value characteristics. The ease with which polyurethane can be produced, used, recycled, and reused without losing value offers a new green solution in designing sustainable polymer materials with a high economic value and a minimal environmental burden.
Ionic COF Composite Membranes for Selective Perfluoroalkyl Substances Separation
Authors: Zhang Wenxiang , Li Yinhui , Xing Zheng , Zhao Minghan , Fu Yu , Wang Shanshan , Wu Yue , Zeng Jiahui , Li Xiaoyu , Ma Heping 
Published: October 2022

AbstractHigh‐performance membranes are critical to membrane separation technology. In recent years, 2D covalent organic frameworks (2D COFs) have attracted extensive attention in the field of membrane separation due to their high porosity, ordered channels, and fine‐tuned pore sizes, which are considered as excellent candidate to solve the trade‐off between membrane selectivity and permeability. Herein, two kinds of ionic 2D COFs with different charge properties (termed as iCOFs) are integrated into polyacrylonitrile (PAN) substrates to form two composite membranes (PAN@iCOFs) with excellent selective perfluoroalkyl substances (PFASs) separation performance with high solvent permeability and good mechanical properties. The as‐prepared PAN@iCOFs composite membranes can selectively reject more than 99.0% of positively and negatively charged PFASs in wastewater while maintaining good stability and recyclability.
Electrospun Nanofiber Electrodes for Lithium‐Ion Batteries
Authors: Zhao Yun , Yan Jianhua , Yu Jianyong , Ding Bin 
Published: October 2022

AbstractElectrospun nanofiber materials have the advantages of good continuity, large specific surface areas, and high structural tunability, which provide many desirable characteristics for lithium‐ion battery electrodes. Here, the principles and advantages of electrospinning technology are first elaborated, then the previous studies on high‐performance nanofibrous electrode materials prepared by electrospinning technology are comprehensively summarized, and the correlation between 1D nanostructured materials and electrode performances is discussed. Finally, the remaining challenges of nanofibrous electrodes are proposed and some future study directions of this particular area are pointed out. This review provides new enlightenment for the design of nanofibrous electrodes toward high‐performance lithium‐ion batteries.