bluuebunny/crossref_metadata_2025_split huggingface dataset:
Respon Sistem Homeostasis Kalsium Tikus Ovariektomi yang Mengkonsumsi Kombinasi Calcitriol dengan Raloxifene
Authors: Hartiningsih Hartiningsih , Anggraeni Devita 
Published: November 2022

Normal range of calcium (Ca) level in the blood is maintained by Ca homeostasis in the intestinal, kidney and bone. The objective of this research was to study the response of calcium homeostasis system ovariectomized rats consuming combination of calcitriol and raloxifene by calcium balance study. Twenty five female Wistar rats at 8 weeks of age were divided into five groups of five: normal control (NK), ovariectomy control (OVK), ovariectomy+calcitriol (OVD ), ovariectomy+ raloxifene (OVR), and ovariectomy+calcitriol+ 20raloxifene supplementation (OVDR), of five each. Seven weeks after the surgery, each rats was placed into individual metabolic cages for Ca balance studies (Ca consumption, feces and urine Ca excretion, and intestinalCa absorption). In 5 to 8 days of the balance studies, the remaining food, urine, and feces were collected every day for Ca analyses. The results showed that Ca consumption, feces and urine Ca excretion, and intestinal Ca absorption of OVDR rats were higher than OVK rats. Consumption and feces Ca excretion of OVDR rats were higher than OVK rats which informed the decrease of estrogen hormone. Intestinal Ca absorption of OVDR rats was higher than OVK rats which informed the decrease of parathyroid hormone. Meanwhile, urine Ca excretionof OVDR rats was higher than OVK rats which informed the decrease of parathyroid hormone and estrogen. In conclusions, the response of calcium homeostasis system in ovariectomized rats consuming combination ofcalcitriol and raloxifene for 8 weeks was marked by increasing Ca absorption in intestine and Ca excretion in urine.
Penggunaan Antigen Mycoplasma gallisepticum Freeze-Thawing dalam Teknik Enzyme-Linked Immunosorbent Assay
Authors: Rachmawati Faidah Rachmawati , Wibawan I Wayan Teguh , Mayasari Ni Luh Putu Ika 
Published: November 2022

Chronic Respiratory Disease (CRD) is a bacterial infection in chickens caused by Mycoplasma gallisepticum. CRD may result in economic loss in the livestock industry, decreasing of egg production and feed efficiency, increasing of medication costs, downgrading of carcass and egg qualities. The aim of this study was to develop the Enzyme-Linked Immunosorbent Assay (ELISA) kit for detecting antibodies of M. gallisepticum using freeze-thawing antigens. The cut-off point was determined by S/N method. Chicken antiserum againts M. gallisepticum was collected every week on 1st – 6th week post immunization of whole cell antigen of M. gallisepticum. Optimization of antigen, serum and conjugate were 10 μg/ml protein concentrations, 1:800 serum dilution and 1:10000 conjugate dilution. The validation results on developed ELISA kit (MyGELISA) analyzed by ROC curve using MedCalc statistical software revealed that developed ELISA kit (MyGELISA) had 100% sensitivity and 86.21% specificity with 95% confidence interval. The results of RSA, MyGELISA and commercial ELISA kit showed antibodies positif againts M. gallisepticum. In conclusion, MyGELISA using freeze-thawing antigen can be used to detect antibodies of M. gallisepticum.
Peran Makromineral pada Reproduksi Ruminansia
Authors: Yanuartono Yanuartono , Indarjulianto Soedarmanto , Nururrozi Alfarisa , Purnamaningsih Hary 
Published: November 2022

Macromineral is one component of a nutrient that has an important role in the growth, health, production,reproduction and immune system of animals. Ruminants need makromineral such as Ca, P, Mg, K, Na , Cl and S. Mineral needs of ruminant affected by several factors such as their age, pregnancy and lactation status.Mineral deficiency can cause disturbances in reproduction ruminant. Ca and P have direct influence while Mg, K, Na, Cl and S acted indirectly on reproductive function. A complete understanding of the role macromineral on ruminant reproductive function is indispensable for the prevention of their reproductive disorders due toimproper feeding minerals.
The Effectiviness of Ketapang (Terminalia cattapa L.) Leave Extract for the Treatment of Aeromonas salmonicida Infection in Catfish (Pangasioniodon hypophthalmus)
Authors: . Sumino , Supriyadi Asep , . Wardiyanto 
Published: November 2022

Abstract Catfish is one of the fish species with certain advantages that attract the farmers interest to culture. The species becomes popular following the successful spawning in the pond. Additionally, catfishes have high economic value due to their rapid growth and response to good artificial feed. Their farming activities are inseparable from concerns about their potential diseases. Diseases in farmed fish can be caused by fungi, parasites, bacteria and viruses. One type of bacterium to take serious control is Aeromonas salmonicida. This pathogenic bacteria may cause furuncle and death of farmed fish. The use of ketapang leaves is effective in inhibiting or killing the pathogenic A. salmonicida in catfish. In the present study, each test used a sample of 10 catfishes of approximately 10 cm in size. Parameters of the test consist of survival rates and pathologic lesions. The feed treatment consisted of ketapang leaf extract in concentrations of 100, 200, and 300 mg / ml, and a positive control and a negative control. Each treatment consisted of 3 replicates, and were analyzed statistically. The results indicated that the LD50 (Lethal Dose), MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) are 107 cfu/ml, 50 mg/ml and 100 mg/ml, respectively. The most effective concentration of ketapang leaf for the treatment of A. salmonicida infection in the catfish is 200 mg/ml. This is indicated by the highest level of catfish survival and the lowest rate of the pathologic lesions.
Influence of Palm Sugar Water in the Native Chicken Performance
Authors: Aryanti Fera , Aji Muhammad Bayu , Budiono Nugroho 
Published: November 2022

Abstract Palm sugar containing 66,18% sukrose is an additional source of energi quickly available to the chicken. A study to examine the effect of palm sugar in the native chicken performance was held in animal health training center, Cinagara-Bogor lasted from August until November 2012. This present experiment using 1274 native chicken that were kept starting DOC. Palm sugar concentrations given in the drinking water as much as one percent started to be given to the chcken when they were still DOC. After that, the palm sugar water was given continously intermittent interval by administering multivitamin. The control group was not given the palm sugar. Chicken aged 0 to 6 weeks were given palm sugar twice a day. After reaching over 6 weeks old, palm sugar water was only given onece a day. Result obtained showed that the administration of palm sugar water affected the increase in body weight gain, feed consumption and lower mortality as well. The result also showed that the FCR is lower than that of the control group until 6 weeks old.
The Effect of Red Fruit's (Pandanus conoideus) Extract to Eritrocytes, Hemoglobin, PCV and TPP Concentrations of Mice (Mus musculus) Infected by Toxoplasma gondii
Authors: Utami Anis Dwi , Priyowidodo Dwi 
Published: November 2022

Toxoplasmosis is a zoonotic parasites caused by Toxoplasma gondii. The red fruit’s extract contains tocopherol and betacarotene as antioxidant and it can repair the bodies immune system. This research was aimed to study the effect of red fruit’s extract to erithrocytes, hemoglobine, the values of PCV and TPP concentration of mice infected with T. gondii, so that the red fruit extract can be used as alternative herbal medicine for eliminating Toxoplasmosis. 15 of Balb/C mice, male, three months, 30 gr of body weight were used as experimental animals. The mice were then devided into three groups. Group I was a control, group II was tachyzoite infected and group III was tachyzoite infected and given red fruit’s extract. Group III given red fruit’s extract on first day with 0,53 g/kg BW/mice/day. Mice in group II and III were infected by injection Toxoplasma gondii tachyziote 1x103 a mice intraperitoneally. The blood sample was taken from five mice in each group at 7th and 12th day pass through cantus medialis. The blood was examinated in Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Data were analyzed by using Factorial 2 x 3. The result showed no significant difference (P>0,05) in hemoglobin, and PCV’s value between groups I, II and III before and after infection of T. gondii’s tachyzoite. The TPP’s value between groups I, II and III before and after infection of T. gondii’s tachyzoite was significantly difference (P<0,05).
Cholesterol Levels In Bligon Goats During Estrous Cycle
Authors: Kartikasari Nadia , Ummami Risa 
Published: November 2022

The study was aimed to assess the status of cholesterol levels during the estrous cycle in goats. The present study was conducted by using 4 female cycling bligon goats (Capra hircus), aged 2-3 years, weighed 27-45 kgs, and clinically healthy and Venous blood sampling was done on days 1 (estrus), 3-4 (early luteal), 10-16 (late luteal), 17-20 (proestrus) using a syringe and collected in tubes containing lithium heparin. Plasma wasseparated by centrifugation, put into 1.5 ml Eppendorf tube and stored at a temperature of -20° C until analysis performed. Cholesterol levels were measured by using spectrophotometric method. Cholesterol concentrations during the estrous cycle were analyzed by General Linear Model Repeated Measure. The average of cholesterollevels in bligon goats is 52.20 ± 11.11 – 64.50 ± 18.46 mg/dl. The results showed that the concentration of cholesterol was between phases during the estrous cycle was not significantly different (P>0.05). However, there was a decreasing tendency of cholesterol concentration at the early luteal phase (52.20 mg/dl) and at the late luteal (53.19 mg/dl) compared to the levels at the follicular phase (65.50 mg / dl at estrus and 59.22 mg / dl at proestrus ). It can be concluded that the plasma level of cholesterol does not change significantly during theestrous cycle.
Prevalency of Nematode in Bali Cattle at Manokwari Regency
Authors: Junaidi Muhammad , Sambodo Priyo , Nurhayati Dwi 
Published: November 2022

This study was conducted from August to October 2006, a disease caused by worms digestive tract is one of the causes of the decline in beef production . The purpose of this study was to assess the number of infected cattle nematode, the nematode types and amount of each type of nematodes in Bali cattle in Manokwari. Based onobservations on the worm eggs, total of 23 individuals (44.23 %) of Bali cattle ( puppies and adults ) infected with nematodes. Most of the infected cow is a cow that as many as 16 puppies cattle (69.56 %), while the remaining 7 animals (30.44 %) is the mother cow. Types of nematodes that infect cattle puppies and adult cattle are Cooperiasp., Bunostomum sp., Mecistocirrus sp. and Trichuris sp. The frequency of attacks in a row is as much as 13 cattle Cooperia sp, Mecistocirrus sp as many as 10 head of cattle, as many as 8 sp bunostomum cows and Trichuris sp. as much as 5 cows. The highest number of eggs in a row is Bunostomum sp. (1520), Cooperia sp. (1280), Mecistocirrus sp. ( 1200) and Trichuris sp. (280). The average number of eggs per gr feces ( EPG ) of 4 species of parasites that infect a row is Bunostomum sp . (190 EPG), Mecistocirrus sp. (120 EPG) Cooperia sp. (98.46 EPG)and Trichuris sp. (56 EPG). As many as 44.23 % of Bali cattle in three districts (Masni, Oransbari dan Prafi) attacked nematodes which are predominantly cattle aged less than one year .
Corrosion resistance of composite NI-P coatings in various aggressive media
Authors: Polukhin D. S. , Goikhenberg Yu. N. , Bodrov E. G. 
Published: November 2022

The paper studies corrosion resistance in highly aggressive media of composite nickel-phosphorus coatings after isothermal annealing at different temperatures accompanied by crystallization. The phase composition of chemically deposited Ni-P coating containing about 1% dispersed SiC was analyzed. Gravimetric method was used to determine the mass loss of the samples as a result of daily exposure to various acids and in a solution of nitric acid with a concentration from 5 to 65%, which is extremely aggressive for Ni-P coatings. At each heat treatment, the steel witness samples were used to determine the microhardness by the Vickers method at a load of 100 g. The dependence of the parameters of the corrosion process on the presence of a dispersed additive and the phase composition of the coating has been established. At low holding times the dispersed phase exhibits a barrier effect reducing crystalline phosphide Ni3P formation during annealing and corrosion resistance; meanwhile, prolonged holding at lower temperatures produces about 70% Ni3P, stable high hardness values and improved corrosion resistance values. Lowering the coating heat treatment temperature in an oxidizing environment reduces the intensity of phosphorus burn-off from the surface and decreases all coating properties. The concentration of nitric acid in the solution at the level of 5-15% is critical and contributes to the dissolution of all coatings, regardless of their composition.The conducted research and revealed regularities made it possible to determine the contribution of the phase composition and presence of the dispersed additive to the formation of the main service characteristics of the nickel-phosphorus coatings - microhardness and resistance to aggressive media, as well as to determine the technological modes of heat treatment that allow the formation of optimum properties of products used in the oil and gas industry.
Sustained Upregulation of Endothelial Nox4 Mediates Retinal Vascular Pathology in Type 1 Diabetes
Authors: Tang Xixiang , Wang Jinli , Abboud Hanna E. , Chen Yanming , Wang Joshua J. , Zhang Sarah X. 
Published: November 2022

<p>NADPH oxidase 4 (Nox4) is a major source of reactive oxygen species (ROS) in retinal endothelial cells (ECs) and is upregulated under hyperglycemic and hypoxic conditions. However, the role of endothelial Nox4 upregulation in long-term retinal blood vessel damage in diabetic retinopathy (DR) remains undefined. Here, we attempted to address this question using humanized EC-specific <em>Nox4</em> transgenic (<em>hNox4</em>EC-Tg) and EC-specific <em>Nox4</em> knockout (<em>Nox4</em>EC-KO) mouse models. Our results show that, <em>hNox4</em>EC-Tg mice at age of 10-12 months exhibited increased tortuosity of retinal blood vessels, focal vascular leakage, and acellular capillary formation. <em>In vitro</em> study revealed enhanced apoptosis in brain microvascular ECs (BMECs) derived from <em>hNox4</em>EC-Tg mice, concomitant with increased mitochondrial ROS, elevated lipid peroxidation, decreased mitochondrial membrane potential (ΔΨm), and reduced mitochondrial respiratory function. In contrast, EC-specific deletion of Nox4 decreased mitochondrial ROS generation, alleviated mitochondrial damage, reduced EC apoptosis, and protected the retina from acellular capillary formation and vascular hyperpermeability in a streptozotocin-induced diabetes mouse model. These findings suggest that sustained upregulation of Nox4 in the endothelium contributes to retinal vascular pathology in diabetes, at least in part, through impairing mitochondrial function. Normalization of Nox4 expression in ECs may provide a new approach for prevention of vascular injury in DR. </p>
Effect of the Viscosity and Application Time of Different Phosphoric Acids on the Bond Strength and Nanoleakage of Fiber Posts to Root Dentin
Authors: Ñaupari-Villasante R , Ocampo JB , Cardenas AFM , Muniz LP , Siqueira FSF , Moreira ACL , Feitosa VP , Loguercio AD 
Published: November 2022

SUMMARY

Purpose
To evaluate the effect of application time and viscosity of meta-phosphoric acid (MPA) and ortho-phosphoric acid (OPA) on the push-out resin-dentin bond strength (PBS) and nanoleakage (NL) at the adhesive-root dentin interface.

Methods and Materials
Ninety-six roots of premolars were endodontically prepared and randomly assigned into groups according to the (i) acid (OPA and MPA), (ii) viscosity (gel and liquid), and (iii) application time (7 and 15 seconds). Fiber posts were cemented to the roots, which were then transversally sectioned into serial slices. The slices (cervical, medium, and apical) were subjected to PBS or NL. Analysis of the dentin etching pattern was performed using scanning electron microscopy (SEM). The PBS (MPa) and NL (%) data were subjected to three-way repeated-measures analysis of variance (ANOVA) and Tukey’s test (α=0.05).

Results
The cross-product interaction was significant for both PBS and NL (p&lt;0.001). No significant difference in the PBS was observed with the use of OPA compared to MPA (p&gt;0.05). Higher PBS values were observed in the groups etched for 15 seconds compared to 7 seconds, regardless of the acid, viscosity, or root third (p&lt;0.05). Lower NL was observed for MPA etching when compared to OPA etching (p&lt;0.05), and these values were not affected by increasing the application time or acid viscosity (p&gt;0.05). A more pronounced etching pattern was observed with OPA than MPA regardless of the acid viscosity.

Conclusion
The use of 40% meta-phosphoric acid promoted adequate bond strength without increasing dentin demineralization or void spaces in the hybrid layer.
Development of Interactive Multimedia for Room Service Subject
Authors: Sikumbang Syaidina Putri , Yulianti Yeni , Riska Nur 
Published: November 2022

In order to create optimal learning, learning media with new variations that are interesting but effective and advanced technology are needed. This study aims to develop learning media in the form of interactive multimedia based on Adobe Flash CS6 that is suitable for room service on course restaurant setting and service. This media is developed by research and development (R&D) and using the APPED (Analysis, Planning, Production, Evaluation, Dissiminate) as the development model. The results which is quantitative data are then converted into desctiptive analysis. The validation results by experts such as: 1) media expert the percentage is 99.2% which means that it is excellent quality, 2) by subject expert the percentage is 95% which means that it is excellent quality, 3) by language expert the percentage is 77.5% which means that it is good quality. For student acceptance responses, the percentage is 89.83% which means it is very positive In conclusion this media is validated to be used with good quality
TEKNOLOGI PENGOLAHAN BUAH JENGKOL DAN PEMASARAN BAGI MASYARAKAT DI DESA SIDO MAKMUR KECAMATAN SIPORA UTARA KABUPATEN KEPULAUAN MENTAWAI
Authors: Thressia Merry , Mulyadi Mulyadi 
Published: November 2022

Jengkol merupakan tanaman asli daerah tropis yang banyak dijumpai di seluruh wilayah Indonesia. Tanaman jengkol termasuk jenis tanaman semak berkayu dengan ketinggian rata-rata mencapai 20 meter dan dapat hidup di daratan tinggi dan dataran rendah dengan buah berwarna coklat dan berbentuk bundar. Desa Sido Makmur merupakan salah satu desa penghasil tanaman jengkol dengan kualitas terbaik yang terkenal sampai ke luar Kabupaten Kepulauan Mentawai, karena rasanya yang enak namun tidak berbau. Setiap musim jengkol di Desa Sido Makmur akan dijumpai buah jengkol yang berserakan di tepi jalan, karena buah jengkol hanya dikonsumsi untuk lauk pauk sehari-hari dan sebagai pembatas pekarangan rumah saja dan tidak termanfaatkan secara maksimal. Melihat kondisi ini, perlu dilakukan pengolahan buah jengkol sehingga bermanfaat dan berdaya guna dan dapat menambah penghasilan masyarakat setempat. Tujuan dari kegiatan ini adalah untuk memberikan pemahaman dan menambah pengetahuan masyarakat sebagai pelaku usaha buah jengkol tentang tata cara pengelolaannya serta cara pemasarannya menggunakan TTG (Teknologi Tepat Guna), seperti; Market Place, facebook, WhatsApp, Telegram, YouTube, dan Instagram, sehingga dari hasilnya dapat meningkatkan pendapatan masyarakat. Pada kegiatan ini metode yang digunakan adalah metode ceramah dan brainstorming dengan memaparkan dan menjelaskan materi pelatihan tentang manfaat tanaman jengkol dan tata cara pengolahannya sekaligus bertukar pikiran dan pengalaman antara narasumber dengan peserta, sehingga dapat menghasilkan berbagai macam kuliner versi buah jengkol seperti kerupuk jengkol, sate jengkol, rendang jengkol, dan nugget jengkol. Kegiatan ini dilaksanakan di aula kantor Desa Sido Makmur yang dihadiri oleh 15 (lima belas) orang peserta yang tergabung dari 3 (tiga) dusun. Hasil dari kegiatan ini adalah 1). Peserta dapat memahami manfaat dari pengolahan buah jengkol, 2). Peserta mampu mengolah buah jengkol menjadi beberapa jenis produk kuliner seperti kerupuk jengkol, sate jengkol, rendang jengkol, dan nugget jengkol, 3). Peserta memiliki keingintahuan yang tinggi untuk perkembangan TTG (Teknologi Tepat Guna), sehingga dapat memasarkan hasil produksi buah jengkol ke luar Kabupaten Kepulauan Mentawai yakni Kota Padang.
Increase of Unsteady Pressure at a Trailing Edge due to Inflow Turbulence
Authors: Botero-Bolívar Laura , dos Santos Fernanda L. , Venner Cornelis H. , de Santana Leandro 
Published: November 2022

This paper presents new insights into the penetration of the freestream turbulence in a turbulent boundary layer. Experiments evidence an increase in the velocity fluctuations and integral length scale across the boundary layer in addition to an increase of the surface pressure fluctuations close to the airfoil trailing edge. This paper proposes a new empirical model to predict the integral length scale inside the boundary layer at the trailing edge of an airfoil subjected to inflow turbulence. The potential increase of the airfoil trailing noise is discussed. The flow mechanism studied in this paper is relevant to the noise prediction of many applications, including vertical-axis wind turbines subjected to high-inflow turbulence winds, generally observed in urban zones. Experiments performed on a NACA 0012 airfoil subjected to inflow turbulence of 10 and 20% intensity measured the boundary-layer turbulence and the surface pressure fluctuations close to the airfoil trailing edge. Measurements showed that the high-inflow turbulence penetrates the turbulent boundary layer reaching the inner sublayer. A freestream turbulence of 10% increases the velocity fluctuations inside the boundary layer caused by the larger turbulent structures, whereas an inflow of 20% turbulence intensity causes the increase of the velocity fluctuations caused by all turbulent scales. Furthermore, the freestream turbulence significantly increases the surface pressure fluctuations at the trailing edge. For an inflow with 10% turbulence intensity, the surface pressure spectrum increases by more than 6 dB in the entire frequency range. The increase is above 10 dB if the inflow turbulence intensity is 20%.