bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors
Authors: Crulhas Bruno P. , Basso Caroline R. , Castro Gustavo R. , Pedrosa Valber A. 
Published: June 2022

Background:
Prostate cancer cells have very high PCA3 messenger RNA levels,
which turns them into one of the new biomarkers for prostate cancer prognosis and diagnosis.

Objective:
Our goal here is to develop a new aptasensor to detect PCA3 release by the
cancer cell.

Methods:
DNA hairpin containing PCA3 aptamer was thiolated, conjugated to methylene
blue (MB) redox probe, and immobilized on gold electrode through self-assembly to
detect label-free cancer cells.

Results:
Our data have evidenced stable and sensitive sensors presenting a wide linear detection
range (0-150ng/mL). In addition, monitoring PCA3 released by different types of
prostate cells can provide in-depth knowledge about prostate cancer dynamics; therefore,
it is a powerful platform for earlier clinical diagnostic. The released PCA3 can vary depending
on the type of adopted prostate cells.

Conclusion:
PCA3 release was monitored in a group of cells for 2 h; it showed significantly
higher expression in both LNCaP and PC-3 cells. This strategy provides a unique
and simple methodology to achieve more sensitive and specific PCA3 detection; thus, it
emerged as a promising tool for early cost-effective diagnosis
Development of a WeChat-based Mobile Messaging Smoking Cessation Intervention for Chinese Immigrant Smokers: Qualitative Interview Study
Authors: Jiang Nan , Rogers Erin S , Cupertino Paula , Zhao Xiaoquan , Cartujano-Barrera Francisco , Lyu Joanne Chen , Hu Lu , Sherman Scott E 
Published: June 2022

Background
Smoking remains a major public health issue among Chinese immigrants. Smoking cessation programs that focus on this population are scarce and have a limited population-level impact due to their low reach. Mobile messaging interventions have the potential to reach large audiences and expand smokers’ access to smoking cessation treatment.

Objective
This study describes the development of a culturally and linguistically appropriate mobile messaging smoking cessation intervention for Chinese immigrant smokers delivered via WeChat, the most frequently used social media platform among Chinese people globally.

Methods
This study had 2 phases. In phase 1, we developed a mobile message library based on social cognitive theory and the US Clinical Practice Guidelines for Treating Tobacco Use and Dependence. We culturally adapted messages from 2 social cognitive theory-based text messaging smoking cessation programs (SmokefreeTXT and Decídetexto). We also developed new messages targeting smokers who were not ready to quit smoking and novel content addressing Chinese immigrant smokers’ barriers to quitting and common misconceptions related to willpower and nicotine replacement therapy. In phase 2, we conducted in-depth interviews with 20 Chinese immigrant smokers (including 7 women) in New York City between July and August 2021. The interviews explored the participants’ smoking and quitting experiences followed by assessment of the text messages. Participants reviewed 17 text messages (6 educational messages, 3 self-efficacy messages, and 8 skill messages) via WeChat and rated to what extent the messages enhanced their motivation to quit, promoted confidence in quitting, and increased awareness about quitting strategies. The interviews sought feedback on poorly rated messages, explored participant preferences for content, length, and format, discussed their concerns with WeChat cessation intervention, and solicited recommendations for frequency and timing of messages.

Results
Overall, participants reported that the messages enhanced their motivation to quit, offered encouragement, and made them more informed about how to quit. Participants particularly liked the messages about the harms of smoking and strategies for quitting. They reported barriers to applying some of the quitting strategies, including coping with stress and staying abstinent at work. Participants expressed strong interest in the WeChat mobile messaging cessation intervention and commented on its potential to expand their access to smoking cessation treatment.

Conclusions
Mobile messages are well accepted by Chinese immigrant smokers. Research is needed to assess the feasibility, acceptability, and efficacy of WeChat mobile messaging smoking cessation interventions for promoting abstinence among Chinese immigrant smokers.
Efficacy and Safety of Intensive Versus Nonintensive Supplemental Insulin With a Basal-Bolus Insulin Regimen in Hospitalized Patients With Type 2 Diabetes: A Randomized Clinical Study
Authors: Vellanki Priyathama , Cardona Saumeth , Galindo Rodolfo J. , Urrutia Maria A. , Pasquel Francisco J. , Davis Georgia M. , Fayfman Maya , Migdal Alexandra , Peng Limin , Umpierrez Guillermo E. 
Published: June 2022

OBJECTIVE
Administration of supplemental sliding scale insulin for correction of hyperglycemia in non–intensive care unit (ICU) patients with type 2 diabetes is frequently used with basal-bolus insulin regimens. In this noninferiority randomized controlled trial we tested whether glycemic control is similar with and without aggressive sliding scale insulin treatment before meals and bedtime in patients treated with basal-bolus insulin regimens.

RESEARCH DESIGN AND METHODS
Patients with type 2 diabetes with admission blood glucose (BG) 140–400 mg/dL treated with basal-bolus insulin were randomized to intensive (correction for BG &gt;140 mg/dL, n = 108) or to nonintensive (correction for BG &gt;260 mg/dL, n = 107) administration of rapid-acting sliding scale insulin before meals and bedtime. The groups received the same amount of sliding scale insulin for BG &gt;260 mg/dL. Primary outcome was difference in mean daily BG levels between the groups during hospitalization.

RESULTS
Mean daily BG in the nonintensive group was noninferior to BG in the intensive group with equivalence margin of 18 mg/dL (intensive 172 ± 38 mg/dL vs. nonintensive 173 ± 43 mg/dL, P = 0.001 for noninferiority). There were no differences in the proportion of target BG readings of 70–180 mg/dL, &lt;70 or &lt;54 mg/dL (hypoglycemia), or &gt;350 mg/dL (severe hyperglycemia) or total, basal, or prandial insulin doses. Significantly fewer subjects received sliding scale insulin in the nonintensive (n = 36 [34%]) compared with the intensive (n = 98 [91%] [P &lt; 0.0001]) group with no differences in sliding scale insulin doses between the groups among those who received sliding scale insulin (intensive 7 ± 4 units/day vs. nonintensive 8 ± 4 units/day, P = 0.34).

CONCLUSIONS
Among non-ICU patients with type 2 diabetes on optimal basal-bolus insulin regimen with moderate hyperglycemia (BG &lt;260 mg/dL), a less intensive sliding scale insulin treatment did not significantly affect glycemic control.
Analisis Sistem Pentanahan di Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang
Authors: Hambali Hambali , Sari Yeza Permata 
Published: June 2022

The ideal grounding resistance to avoid from lightning strikes is R < 1 Ω . This study aims to measure grounding resistance and analyze the resistance grounding at different depths. The research was conducted in the Integrated Class Building and Chemistry Laboratory Building, Faculty of Mathematics and Natural Sciences, Padang State University (FMIPA UNP). The type of research used is an experiment by direct measurement to measure the grounding resistance at the grounding terminal on the box electrical and measuring ground resistance using solid electrodes with a diameter of 5/8 inches at different depths. Measurements were carried out using the three-point method using an Earth Tester. Based on research conducted, the average value of ground resistance in the Integrated Class Building, Department of Mathematics1and1Science, Padang State University is 1.85 Ω and the average value of ground resistance in the Chemical Laboratory Building is 9.38 Ω. The recommended ground system was installed is grounding single1rod with ground resistance 0.91 Ω at the Integrated Class Building, Faculty of Mathematics and Natural Sciences, Padang State University and R = 0.95Ω at the Chemistry Laboratory Building, Faculty of Mathematics and Natural Sciences. Padang State University.
Perancangan Sistem Pencampuran Cat Berbasis Mikrokontroler
Authors: Rahmandika Try Andio , Eliza Fivia 
Published: June 2022

Sistem kendali (control system) adalah suatu alat yang digunakan untuk mengendalikan, memerintah, dan mengatur keadaan dari suatu sistem. Banyak peralatan industri yang memanfaatkan kemajuan di bidang pengendali sebagai upaya pengaplikasian kendali otomatis, hasil yang didapatkan dari peralatan yang bekerja secara otomatis mempunyai kualitas dan ketelitian sehingga didapatkan hasil yang konstan dan sesuai dengan yang diinginkan. Tugas akhir ini bertujuan untuk merancang sistem yang dapat memadukan warna cyan, magenta, yellow, black menjadi warna yang bervariasi. Rancangan Tugas Akhir ini mengunakan Arduino Uno 328 sebagai pengendali dan pengontrol pencampuran warna cat, untuk pengatur keluaran aliran cat pada masing-masing warna dasar di atur menggunakan Visual Basic dan motor servo bertindak sebagai katup untuk mengatur keluaran cat. Perancangan alat pencampuran cat ini dilakukan pada komputer (PC) dengan menggunakan software visual basic sebagai media tampilan proses pencampuran cat dan pengontrolan pilihan warna cat yang akan dilakukan pencampuran. Berdasarkan hasil pengujian dan analisa Dari hasil pembuatan tugas akhir Perancangan pencampuran cat otomatis berbasis mikrokontroler Atemega328 bahwa Setelah dilakukannya pengujian sebanyak tiga kali dari beberapa warna, maka hasil warna yang didapat tidak berbeda/ mendekati dan Software dalam rancangan sistem pencampurat cat mampu melakukan pencampuran cat sesuai dengan nilai set point dengan pemberian delay pada buku/tutup katup didapatkan volume yang sesuai dengan yang ditentukan serta mampu mencampur warna cat sesuai yang dikehendaki.
Strategi Pelatih Futsal Dalam Komunikasi Kelompok Untuk Mengangkat Citra Futsal Wanita Indonesia Di Tifosi AMFC Club
Authors: Siwi Shandra Mutiara , Dunan Amri 
Published: June 2022

Penelitian ini membahas mengenai strategi pelatih futsal dalam komunikasi kelompok untuk mengangkat citra futsal putri indonesia di Tifosi Amfc Club. Penulis ingin mengetahui dan menganalisis bagaimana strategi pelatih futsal putri dalam komunikasi kelompok untuk mengangkat citra futsal putri indonesia di Tifosi Amfc. Sebagai landasan dari penelitian ini, teori yang digunakan yaitu Teori Development Model dan teori citra Frank Jefskins. Teori ini membantu peneliti dalam mencari tahu lebih jauh tentang strategi pelatih futsal putri Tifosi Amfc dan memperkuat hasil penelitian. Penulisan ini menggunakan metode kualitatif dan untuk pengumpulan data menggunakan wawancara secara mendalam, observasi, dokumentasi dan studi pustaka. Di dalam pelaksanaan strategi pelatih futsal putri Tifosi Amfc yang dilakukan adalah menjalankan teori citra yang terdiri dari persepsi, kognisi, motivasi dan sikap dan juga menerapkan komunikasi kelompok sebagai metode yang digunakan untuk mencari informasi mengenai kepercayaan, kualitas, dan cara berkomunikasi dalam mengembangkan citra futsal putri. Pada perencanaan ini, pelatih futsal putri Tifosi Amfc Jakarta menunjukan perannya sebagai fasilitator komunikasi dengan menerapkan berbagai upaya agar dapat mengangkat citra futsal putri. Berdasarkan hasil yang didapat dari wawancara bahwa dalam mengangkat citra futsal wanita melalui komunikasi kelompok seorang pelatih harus memiliki kepercayaan, kualitas, kemampuan penyampaian komunikasi.
DZIKRUL GHOFILIN SEBAGAI MEDIA DAKWAH GUS MIEK
Authors: Setiawan Muhamad Agung 
Published: June 2022

One way to get closer to Allah is through dhikr. Dhikr may let the mind calm and the heart becomes peaceful. One of the islamic preachers who uses dhikr as his da'wah media is Gus Miek, the son of the great cleric of the Al-Falaah Ploso Islamic boarding school in Kediri, namely KH. Djazuli Uthman. The dhikr media that was initiated by Gus Miek which is still eloquently enlivened is dhikrul ghofilin. The purpose of this study is to explain how dhikrul ghofilin is a media for Gus Miek's da'wah. To get the results of this study, the researcher uses a qualitative method and literature review as the source of the study. The results of this study state that Gus Miek in establishing dhikrul ghofilin as his da'wah media was very successful in attracting Mad'u from all society, the ordinary people, the clerics to the sinners, although there were previously twists and turns that happened because of the controversial founder.
Keywords: Dhikr, Da'wah, Media
Utilization of Gonadotropin-Releasing Hormone Agonists in the Treatment of Metastatic and Locally Advanced Prostate Cancer - Comparisons of Practices from Three European Countries
Authors: Milovanović Ivan , Milutinović Filip , Đorđević Đorđe , Mihajlović Filip , Bančević Vladimir , Milovanović Dragan 
Published: June 2022

Abstract
Introduction: Prostate cancer is one of the most common health threats for men in the developed world. With the advent of prostate cancer screening using serum prostate-specific antigen (PSA) tests, prostate cancer mortality has declined at the expense of substantial disease overtreatment. Modern prostate cancer therapy is performed according to certain guidelines. Antiandrogens are compounds that inhibit the action of androgens in prostate cancer cells by blocking receptors and preventing the binding of hormones to them. Aim: The aim of this research is to analyze the use of registered forms of LHRH agonists used in the treatment of locally advanced and metastatic prostate cancer in the last five years to examine trends in prescribing this group of drugs in Serbia whose patients gravitate towards the Urology Clinic at the UCC Kragujevac. Material and method: Using the ATC/DDD methodology, the use of LHRH agonists at the Urology Clinic of the UCC Kragujevac. A retrospective study of the use of this group of drugs according to the ATC classification was performed on the basis of data obtained from the hospital pharmacy for the period from year 2016 to year 2021, and the results are expressed by the number of DDD per 1000 inhabitants per day (1000/inhabitants/day). In the observed period, 1361 patients with a diagnosis of C61 (malignant prostate tumor) were treated at the Clinic of Urology. Results: In the observed period, a preparation containing triptorelin in a dose sufficient for one month of therapy was most often used. The total consumption of gonadorelin was lower compared to the rest of Serbia and EU countries, which was expected due to the protocol and the number of patients who gravitated towards the UCC Kragujevac. Conclusion: Despite certain limitations, this evaluation represents the first attempt to summarize the available evidence on the prescribing of LHRH agonists in Serbia. It was found that the consumption in UCCKG is lower compared to the consumption of these drugs in Serbia, Croatia and Italy at the same time intervals, for the same observed diagnosis.
Efficacy of shockwave C2 coronary intravascular lithotripsy for management of severely calcified left anterior descending stenosis
Authors: Sharma Sanjay Kumar` 
Published: June 2022

Severely calcified coronary lesions remain a formidable hurdle for percutaneous coronary intervention as it averts the stent delivery and complete stent apposition. To overcome this Achilles heel of an interventional cardiology, a current US FDA approved method named shockwave intravascular lithotripsy (S-IVL; Shockwave Medical, Inc) is used for its treatment. Herein, we report a case of a 53-year-old dyslipidemic male with severely calcified left anterior descending artery (LAD) lesion that was successfully treated with S-IVL and drug-eluting stent implantation. At first, the patient was presented with complaints of chest pain and breathlessness. His coronary angiogram revealed heavily calcified stenosis in diffuse mid LAD—completely totally occluded mid-diagonal (D) 1 that was checked through retrograde filling via collateral and calcified stenosis in ostial D2, mild stenosis in ostial left circumflex and mild stenosis at distal before crux 50% stenosis in dominant right coronary artery. Following this, it was decided to proceed with primary percutaneous coronary intervention with S-IVL to LAD. After successful delivery of S-IVL to LAD, a Tetriflex sirolimus eluting stent (Sahajanand Medical Technologies Ltd., Surat, India) was successfully implanted at LAD. Finally, the patient’s condition was stabilized and discharged after two days of index procedure.
BIOLOGIA DEL MINADOR DE LAS HOJAS DELCRISANTEMO Liriomyza trifolii (Burgess)
Authors: MORA-M. HOMERO R. , MOSQUERA-P. FELIPE 
Published: June 2022

Estudios sobre Ia biologia del minador del crisantemo Liriomyza trifolii (Bur­gess) mostraron que los huevos son puestos debajo de la epidermis del haz de la hoja; la eelosiOn de las larvas de primer instar ocurre entre 6-8 dias des­pues de la oviposiciOn. La fase larvaria tiene tres instares, los cuales pueden diferenciarse por el tamano de los gan­chos bucales; las larvas completan su desarrollo en 14-31 dias; la prepupa tiene una duraciOn de 5-8 horas y, la pupa de 15-18 dias. La longevidad de las hembras adultas fue en promedio de 11 dias y, la de los machos de 5 dias. Las hembras copulan desde el d fa de su emergencia y pueden realizar varias copulas con diferentes machos. La oviposiciOn se inicia a partir del Se­gundo dia, alcanzando el pico al quin­to dia. Entre el dia 5o. y 100., las hembras depositan el 74% de los hue­vos viables.
Comportamiento de Epidinocarsis diversicornis (Howard) y Acerophagus coccois Smith (Hymenoptera: Encyrtidae), parasitoides de dos piojos harinosos de la yuca, Phenacoccus herreni y Ph. gossypii (Townsend & Cockerell) (Homoptera: Pseudococcidae)
Authors: BELLOTTI ANTHONY C. , HERRERA-F. CARLOS J. 
Published: June 2022

Los parasitoides E. diversicornis y A. coccois se han encontrado en forma abundante en los Departamentos del Meta (Carimagua) y Valle del Cauca (CIAT-Palmira), respectivamente, ata­cando P. herreni y P. gossypii en el cultivo-de la yuca. Con el objetivo de comparar el com­portamiento de los dos himenopteros, se analizaron las siguientes situaciones: estado de desarrollo de P. herreni preferido por los citados parasitoides, respuesta de encapsulación de los pio­jos, mortalidad de P. herreni por el efecto del parasitismo en condiciones de laboratorio (25°C y 70% HR), el efecto de la temperatura (20°, 25° y 30°C) en el desarrollo de ambos ene­migos naturales para estimar el tiempo fisiológic.3 o calor acumulado que ne­cesita para completar el desarrollo dado en grados-día (°D) y el umbral mínimo de desarrollo. El estudio permitió comprobar que el estado de desarrollo del piojo preferi­do por los pai asitoides es el tercer ins­tar y la hembra adulta. En cuanto a la encapsulación, se verificó que P. he­rreni encapsula a E. diversicornis, pero no a A. coccois, y que P. gossypii no encapsula ninguna de las dos especies de parasitoides E. diversicornis produ­ce mortalidad en el primer instar de P. herreni, mientras que A. coccois no causa este efecto sobre el piojo. El tiempo desde la oviposíción hasta la emergencia del adulto fue de 35,7; 20,2 y 18,1 días en promedio para E. diversicornis y, de 39.5; 18,5 y 19,7 días en promedio para A. coccois a no, 25° y 30°C, respectivamente. Además, se encontró que, para su desa­rrollo, E. diversicornis requiere 230°D y E. coccois, 175°D.
EVALUACION DEL HONGO Sporothrix insectorum (Hoog, Evans) EN LA CHINCHE DE ENCAJE Leptopharsa gibbicarina (Froeschner) EN PALMA AFRICANA DE ACEITE (Elaeis guineensis Jacq.)
Authors: ORDÓÑEZ ANA ISABEL , GENTY PHILLIPE 
Published: June 2022

El complejo Leptopharsa pestalotiop­sis es un problema endémico en las plantaciones de Palma Africana de Aceite las zonas Central y Norte de Colombia y es responsable, hasta del 60%, de la pérdida en el rendimiento del cultivo. En las plantaciones de caucho del Bra­sil, se encontró que el hongo entorno-patógeno Sporothrix insectorum con­trola, en un 98%, las poblaciones de L. hevea (Tingidea). Este hongo fue introducido a Colombia por Indupal­ma (San Alberto, Cesar), para probar su patogenicidad en Leptopharsa gibbi­carina. Se comparó el crecimiento del hongo en cuatro medios de cultivo (Sabou­rand - maltosa - agar más 1°/o de ex­tracto de levadura (SMAE); papa - dex­trosa - agar (PDA) más Panvit (sales minerales); medio semisintético enri­quecido con sales y el medio comercial 5467, específico para hongos entorno-patógenos). Se usó un diseño comple­tamente al azar con cuatro repeticio­nes y arreglo factorial 2 (cepas de hon­gos) x 3 (temperaturas: 20, 37 y 32°C). S. insectorum crece bien en todos los medios probados a 27 y 30°C (65°/o HR) siendo la óptima a 27°C. Para las pruebas de patogenicidad bajo condiciones de laboratorio, se usaron 5 concentraciones del hongo (10, 105, 106, 107, 108), tratando 100 adultos de L. gibbicarina por tratamiento, bajo un diseño completamente al azar. Se probó la alta acción patogénica del hongo en todas las concentraciones en­sayadas y en comparación con el tes­tigo (10 ml de agua). Los porcentajes de mortalidad fueron: 1,3; 73,5; 73,4 y 72.20/o, los cuales no muestran dife­rencias significativas entre las distintas concentraciones del hongo que se uti­lizaron. Bajo condiciones de campo, se probó la patogenicidad de 2 cepas del hongo (una en colección y otra recientemente importada), usando un diseño comple­tamente al azar con cuatro repeticio­nes a las mismas concentraciones del experimento anterior y se depositaron en una hoja de palma por planta den­tro de jaulas de tul con cremalleras (1,20 m de largo x 40 cm de diáme­tro). Se usaron 20 palmas distribuidas en 20 líneas de la parcela. Las diferen­cias en porcentaje de mortalidad entre los tratamientos (concentraciones) y el testigo fueron altamente significativas para ambas cepas de hongo. Para aplicaciones de campo, es conve­niente usar concentraciones del hongo a 107 y 108 conidias/ml. Los resulta­dos obtenidos muestran las amplias po­sibilidades del uso de S. insectorum para control de ninfas y de adultos de L. gibbicarina en palma africana de aceite. Sin embargo, se deberán hacer más investigación en otras áreas agro-ecológicas y el hongo, posiblemente, pueda utilizarse contra otros Tingidae de importancia económica en otros cultivos.
AISLAMIENTO, IDENTIFICACION Y CARACTERIZACION DE UN VIRUS EN LARVAS DE Euprosterna elaeasa Dyar. (Lepidoptera: Limacodidae)
Authors: PEDRAZA-V. JORGE , LUQUE-Z. EMILIO , FRANCO-B. PEDRO NEL 
Published: June 2022

De larvas Euprosterna elaeasa Dyar, con síntomas de una infección bacte­rial y/o viral, recolectadas en la planta­ción de Palmas Oleaginosas Bucarelia S.A." (Puerto Wilches, Colombia), se efectuó un estudio bacteriológico y vi­rológico de los agentes causantes de dichos síntomas. En el caso de infección bacterial, se aislaron los gérmenes Serratia marces­cens Bizio y Bacillus subtilis Cohn, los cuales se caracterizaron e identificaron mediante observaciones macroscópicas y la realización de pruebas biquímicas. Por primera vez en Colombia, se aisló un Virus de la Poliedrosis Nuclear de tipo Múltiple (M V P N) en E. elaeasa, mediante ultracentrifugación en gra­dientes de sacarosa y se identificó por microscopía electrónica y eiectrofore­sis. Así mismo, se realizó una secuen­cia general de la infección a nivel histo­pato lógico.Para probar la patogenicidad del virus aislado, se realizó un bioensayo con larvas de III, IV y V instar de E. elaea­sa, las cuales se inocularon con 6 con­centraciones del virus. Con los porcen­tajes de mortalidad obtenidos, se reali­zó un análisis de regresión por el método "probit" y se calculó la dosis letal media (DL50), obteniéndose un valor de 1,24 pol/mm2 y 13,49 pol/mm2, para larvas de IV y V instar, respecti­vamente. Se confirmó la patogenicidad del virus y se concluye que, a medida que au­menta el instar larval, se requiere de un DL 50 mayor.