bluuebunny/crossref_metadata_2025_split huggingface dataset:
JAK/STAT3 Pathway Promotes Proliferation of Ovarian Aggregate-derived Stem Cells in vitro
Authors: Saber Maryam , Shekari Faezeh , Mousavi Seyed-Ahmad , Moini Ashraf , Miri Monireh-Sadat , Esfandiari Fereshteh 
Published: April 2022

Abstract
Background
The accurate identification and isolation of ovarian stem cells from mammalian ovaries remain a major challenge because of the lack of specific surface markers and suitable in vitro culture systems. Optimized culture conditions for in vitro expansion of ovarian stem cells would allow for identifying requirements of these stem cells for proliferation and differentiation that would pave the way to uncover role of ovarian stem cells in ovarian pathophysiology. Here, we used three-dimensional (3D) aggregate culture system for enrichment of ovarian stem cells and named them aggregate-derived stem cells (ASCs). We hypothesized that mimicking the ovarian microenvironment in vitro by using an aggregate model of the ovary would provide a suitable niche for the isolation of ovarian stem cells from adult mouse and human ovaries and wanted to find out the main cellular pathway governing the proliferation of these stem cells.
Results
We showed that ovarian aggregates take an example from ovary microenvironment in terms of expression of ovarian markers, hormone secretion and supporting the viability of the cells. We found that aggregates-derived stem cells proliferate in vitro as long-term while remained expression of germline markers. These ovarian stem cells differentiated to oocyte like cells in vitro spontaneously. Transplantation of these stem cells in to chemotherapy mouse ovary could restore ovarian structure. RNA-sequencing analysis revealed that interleukin6 is upregulated pathway in ovarian aggregate-derived stem cells. Our data showed that JAK/Stat3 signaling pathway which is activated downstream of IL6 is critical for ovarian stem cells proliferation.
Conclusions
We developed a platform that is highly reproducible for in vitro propagation of ovarian stem cells. Our study provides a primary insight into cellular pathway governing the proliferation of ovarian stem cells.
Early Change In Platelet Count And MPV Levels Of Patients Who Received Hemodialysis For The First Time: Mogadishu Somalia Experience
Authors: Sarı Öznur , Bashir Ahmed Muhammad 
Published: April 2022

Abstract
IntroductionMean platelet volume (MPV) is a marker used to assess the platelet's size and is also an indicator of platelet reactivity and prothrombic status.ObjectiveIn this study, we aimed to determine the relationship between MPV and biochemical parameters in patients who had received HD for the first time and then in respect of those same patients after their fourth HD.Method151 HD patients were enrolled in this study. Patients were eligible for inclusion if they had received their first HD session during this study protocol. Blood samples for measurement were collected pre-hemodialysis. Most laboratory values, including mean platelet volume (MPV) level and Plt count, were measured before the first HD and after the 4th HD session for each patient.ResultsAmong the patients in our study, the mean age profile of the male patients (n= 103; 68.2%) was found to be higher than that of the female patients (n = 48; 31.8%) (53.62±18.19 vs. 46.17±17.9 years) (p = 0.019).In the patients' laboratory results after the fourth HD session, MPV, MPV/Plt, and Na values had ​​increased to those after the first HD session (p<0.001). When age and gender status were taken into account, the level of weak positive correlation with WBC, neutrophile, and RDW was found, while the weak negative correlation with platelet to lymphocyte ratio (PLR) was found (p<0.001). ConclusionsIn our study, we found that increase in MPV and MPV/PLT levels was significant in the fourth HD session of patients with CKD. It is also debatable that there are findings indicating an increase in platelet reactivity in the first weeks of the onset of HD. This could be an early indicator of the early prevention of cardiovascular diseases.
Topical application of cream containing enriched-schizandrin from Schisandra chinensis fruit exert anti-photoaging efficacy in ultraviolet B- irradicated hairless mice
Authors: Yun Mi-Young , Kim Nam-San , Choi Hwa-Jung 
Published: April 2022

Abstract
Excessive ultraviolet (UV) B irradiation induced skin photoaging. The aim of this study was to evaluate the anti-photoaging efficacy of enriched-schizandrin from Schisandra chinensis fruit in UVB-induced hairless mice. The cream containing enriched-schizandrin from S. chinensis fruit manufactured with another cosmetic ingredient. UVB-photoaged hairless mice topically applied with the cream once a day for 5 weeks. Application of the cream showed normal changes of body weight and food efficiency in the UVB-photoaged hairless mice. The cream application also was decreased interleukin (IL)-1β, matrix metalloproteinase (MMP)-2 and MMP-9 mRNA expressions and then it inhibited MMP-2 protein expression in UVB-photoaged hairless mice. Furthermore, the cream application inhibited epidermal wrinkle formation and decreased wrinkle depth and it restored to wrinkle thickness and collagen degradation of skin in UVB-photoaged hairless mice. Therefore, the cream could recover photoaging generated by UVB irradiation via downregulation of IL-1β, MMP-2, MMP-9 mRNA expressions and suppression of expression of MMP-2 proteins.
Rheological behavior of oil- silicon dioxide- multi walled carbon nanotube hybrid nanofluid: Experimental study and neural network prediction
Authors: Sepehrnia Mojtaba , Mohammadzadeh Kazem , Rozbahani Masoud Hemmati , Ghiasi Mohammad Javad , Amani Mohammad 
Published: April 2022

Abstract
Hybrid nanofluids have great potential for use in thermal systems due to their improved thermal properties. In this paper, the rheological behavior of oil (5w30)- 10% multi walled carbon nanotubes (MWCNT)- 90% silicon dioxide (SiO2) is experimentally examined in the temperature range of 5°C to 65°C. The volume fractions (VFs) are in the range of 0.05 to 1 vol.% and the shear rate (SR) range is 665.5-13330 1/s. Measuring viscosity at different SRs indicated a pseudoplastic rheological behavior of the nanofluids in all VFs and temperatures. Measurement results show that the dynamic viscosity in different volume fractions is reduced when the temperature is increased from 5°C to 65°C. In addition, when the VF is increased from zero to 1%, the dynamic viscosity is augmented between 30.43% and 70.55%. Based on obtained data, a novel three-variable correlation for relative viscosity is proposed which estimates experimental results with a good accuracy. Then, the correlation results are compared to available correlations for hybrid nanofluids in the literature. Finally, a GMDH-type neural network model based on experimental data is developed to predict the relative viscosity of oil (5W30)/SiO2- MWCNT hybrid nanofluids which reveals the predictability of studied hybrid nanofluid using GMDH-type neural network.
First investigation of the effect of strontium oxide on the structure of phosphate glasses using molecular dynamics simulations
Authors: Bouabdalli El Mahdi , Jouad Mohamed El , Hajjaji Abdelowahed , Touhtouh Samira 
Published: April 2022

Abstract

Phosphate glasses are characterized by properties distinguished by comparison with those of others such as borates, silicates, and tellurides that can be used in many fields such as optics, electronics, medicine, environmental protection, etc. On the other hand, these pure phosphate glasses have low chemical durability, which limits their application. The addition of strontium to the glass considerably improves its durability. Using classical molecular dynamics simulations, this work investigated the influence of SrO addition on a glass phosphate structure with a nominal chemical composition of (100- x) P2O5 – x SrO (where x = 0, 5, 10, 15, ... 45 mol %). According to the progressive increase in the SrO content, the density of the simulated glass increased from 2.400 g/cm3 to 2.937 g/cm3, while its molar volume values reduced from 59.143 to 42.449 cm3/mol. The simulated glasses' short-range structure is described, and it agrees well with prior experimental and theoretical research. The increased SrO level in the glass resulted in the depolymerization of the phosphate network, according to simulations. Furthermore, we evaluated the aggregation parameters of the Sr-Sr pair, and it was most intense for the low SrO content, which can lead to the inhomogeneity of the phosphate glass network.
Component content and antioxidant activity of different extracts of cigarette ethanol extract
Authors: LI Wu-na , RAO Gui-wei 
Published: April 2022

Abstract

In this paper, the contents of total flavonoids, total phenols, and antioxidant activity of different extracts of cigarette ethanol extract were studied, and their correlation was to be analyzed. The contents of total flavonoids were determined by Al(NO3)3-NaNO2 colorimetric method, and the contents of total phenols were determined by the Folin-phenol method. 1,1-diphenyl-2-picrylhydrazyl(DPPH) method and pyrogallol autoxidation method were used to study the antioxidant activities of each extract. Last, the correlation between antioxidant activities and the contents of each extract was analyzed. The results showed that the contents of total flavonoids (28.34 ± 0.20mg/g, 29.49 ± 0.14mg/g) and total phenols (24.89 ± 0.06mg/g, 24.44 ± 0.08mg/g) in ethyl acetate and n-butanol fractions were the highest. The scavenging rates of DPPH and superoxide anion of ethyl acetate and n-butanol fractions in IC50 were also the best. Correlation analysis showed that there was a positive correlation between the antioxidant activity and its content. Different extracts of cigarette ethanol extract contain total phenols, total flavonoids and antioxidant activity, among which the ethyl acetate extract is the best.
Parasitic Contamination of Soil, Irrigation Water and Rawly Consumed Vegetables in Farmlands of Asmara, Eritrea
Authors: Fessehaye Nahom , Kesete Yafet , Mohommed-Shifa Sara , Adhanom Samuel , Amanuel Danait , Weldu Million , Mengsteab Naemi , Suleiman Omer , Tesfamariam Adiam , Tseggai Ruth 
Published: April 2022

Abstract
Background
About one third of the world population are infected with intestinal parasites. Eating raw vegetables is customary in many parts of the world including Eritrea posing a great risk for transmission of pathogens. Therefore, the aim of this study was to determine the prevalence and risks of parasitic contamination in raw vegetables, soil and irrigation water samples.
Methods and materials:
A cross-sectional study was carried out on different types of 336 vegetable samples (lettuce, carrot, cabbage and arugula), 60 soil and water samples each using sedimentation technique. Simultaneously, socio demographic and farmer practice data of 60 farmers were collected using structured questionnaire. The obtained data were entered and analysed using SPSS version 20.
Result
The magnitude of contamination of vegetable, soil and water samples was 144(42.9%), 21(35%) and 30(50%) respectively. Lettuce was the most contaminated sample (51.2%) and arugula (50.9%), carrot (42.7%) and cabbage (34.1%). The most prevalent parasites detected was S. stercolaris 30.6% followed by A. lumbricoides (11.8%) and hookworm (11.8%). The main findings also include the statistically significant difference in rate of contamination observed among various farmlands, vegetable types, and farmer practices. Also, significant association were observed between vegetable contamination and source of irrigation water (p-value = 0.02) and area of collection (p-value < 0.001).
Conclusion
This study conclusively reveals the high burden of vegetable, soil and water contamination and the impact of wastewater irrigation system as major risk factor in transmission of intestinal parasitosis among farmers and communities in Asmara.
Assessment and appliction ofWhole-herd-Sampling, qPCR-based-Testing, and Precision-Removal method in Eliminating ASFv in Four Large Swine Herds in China
Authors: Yan Zhichun Jason , Li Xiaowen , Wu Weisheng , Li Peng , Li Junxian , Gao Wenchao , Yu Jincheng , Fan Mingyu , Kang Lixiang , Wang Yunzhou , Yuan Guiqiang , Yu Qiannan , Ren Jing , Yan Zhong , Zhu Liqiang , Li Jintao , Zhang Xiaoyang , Shao Zhenwen , Liu Qingyuan , Zheng Weiwei , Fan Shiran , Huang Yakuan , Chen Jing , Deen John 
Published: April 2022

Abstract
Background Since the first report of ASFV in China in 2018, conventional whole herd depopulation method to control ASF has proved unwieldly because of high production intensity and complex trade network. To provide an alternative to conventional methods, we evaluated the feasibility of implementating an extensive sampling method and qPCR tests to determine the status of ASFV in herds, with a rapid response to identified outbreaks. Case presentations By assessing and applying these methods without whole herd depopulation, we successfully controlled ASF and eliminated the virus from 4 large swine herds. The time to negative herd was 19, 28, 14, and 1 days in farms 1-4 respectively. Retention rates of pigs of farm 1 to farm 4 was 69.7% , 65%, 99.4% and 99.72% respectively.Conclusions We anticipated that this innovative method would replace the conventional stamping out one and greatly facilitate the control and eradication of ASFV in China and worldwide.
IMPLEMENTASI MANAJEMEN HATI SEBAGAI UPAYA PENINGKATAN SUMBER DAYA MANUSIA DI MAYANGKARA GROUP
Authors: Bariroh Muflihatul 
Published: April 2022

Penelitian ini berupaya memaparkan salah satu strategi dalam peningkatan manajemen sumber daya manusia di Mayangkara Group yakni praktik manajemen hati. Jenis penelitian ini adalah kualitatif dengan menggunakan studi pendekatan studi kasus. Hasil penelitian menunjukkan bahwa terdapat beberapa program kegiatan yang wajib diikuti oleh seluruh karyawan sebagai wujud dari implementasi manjamen hati. Manajemen hati merupakan suatu upaya pengelolaan hati untuk seluruh karyawan yang bertujuan untuk membentuk karaktek SDM yang berbudaya dan bernilai ketuhanan. Hati yang terkelola secara baik, akan terlahir SDM berkualitas yang menjadikan bekerja sebagai prioritas beribadah dan tidak hanya untuk mencari profit semata. SDM yang memiliki kualitas hati yang baik, maka segala apa yang menjadi kewajiban karyawan akan menjadi suatu pekerjaan yang menyenangkan, tanpa paksaan dan tentunya bernilai ibadah.
Niobium speciation in minerals revealed byL2,3-edges XANES spectroscopy
Authors: Bollaert Quentin , Chassé Mathieu , Elnaggar Hebatalla , Juhin Amélie , Courtin Alexandra , Galoisy Laurence , Quantin Cécile , Retegan Marius , Vantelon Delphine , Calas Georges 
Published: April 2022

AbstractThe systematic mineralogy of niobium (Nb) is complex, with more than one hundred species dominated by multicomponent oxides of similar chemistry. The determination of Nb speciation in solids (i.e., the distribution between the phases and the crystal-chemical environment of Nb) is thus a challenge in geological contexts. Here, we present the first Nb L2,3-edges X-ray absorption near-edge structure (XANES) measurements on various Nb minerals and synthetic oxides with geological relevance. The interpretation of Nb L2,3-edges XANES spectra in the light of crystal-field theory shows the sensitivity of spectra to local site symmetry and electronic environment around Nb atoms. Crystal-field multiplet simulations give estimates of the 10Dq crystal-field parameter values for Nb5+, which range from 2.8 to 3.9 eV depending on Nb coordination and Nb—O distances. Rather than a 10Dq vs. R–5 relationship (where R represents the average Nb-O bond distance) expected in a point-charge model, we find a R–3 dependence with the crystal-field splitting for reference materials with octahedrally coordinated Nb. Complementary ligand-field multiplet simulations provide evidence of charge transfer between Nb and O. The contribution of the ionic and covalent characters to the Nb-O bonds is equivalent, unlike more ionic 3d metal–O bonds. This systematic characterization of the L2,3-edges XANES spectral properties of Nb provides information on the mechanisms by which Nb5+ substitutes for Fe3+, Ti4+, or Ce4+ in oxides common in geological contexts. Whereas the substitution of Nb5+ for Ce4+ does not modify the local structure of the cation site in cerianite, the substitution of Nb5+ for Ti4+ in rutile and anatase results in an increase of the cation-ligand distance and a decrease in the symmetry of the cation site. Conversely, the substitution of Nb5+ for Fe3+ in hematite and goethite results in a smaller cation site distortion. Our study demonstrates the usefulness of L2,3-edges XANES spectroscopy to determine Nb speciation in minerals to understand the processes of enrichment of this critical metal.
Assessment of accessibility and hydraulic performance of the water distribution system of Ejere Town
Authors: Tufa Girma , Abate Brook 
Published: April 2022

AbstractWater is necessary for human health, economic success, and happiness, as well as other developmental goals such as proper diet, gender equality, education, and poverty reduction. Drinking water is a common problem in Ejere Town. The primary goal of this study was to investigate the accessibility of the town's drinking water distribution system. To achieve this research goal, the hydraulic performance of the town's water distribution system and the level of customer satisfaction were assessed. 374 sampled households were randomly chosen from a total of 13,380 to measure the degree of customer satisfaction with the town's water supply services, and the hydraulic performance of the town's water distribution system was analyzed using the WaterGEMS software. Of the 374 customers surveyed for the study, 85 (22.7%) were satisfied and 289 (77.3%) were dissatisfied, implying that the satisfaction levels were below average. The WaterGEMS hydraulic model was calibrated (R2 = 0.94) using measured data from nine randomly chosen nodes. According to the model results, a great amount of the velocity and pressure in the water distribution system was below the minimum recommended limits. These minimum pressures and velocity levels indicate that there is not enough water pressure in the distribution network to reach all parts of Ejere Town. Finally, it was concluded that there was insufficient access to drinking water in the town.
Modelling annual maximum daily rainfall with the STORAGE (STOchastic RAinfall GEnerator) model
Authors: Petroselli Andrea , De Luca Davide Luciano , Młyński Dariusz , Wałęga Andrzej 
Published: April 2022

Abstract
In this work, the capability of STORAGE (STOchastic RAinfall GEnerator) model for generating long and continuous rainfall series for the upper Vistula basin (southern Poland) is tested. Specifically, in the selected area, only parameters of depth–duration–frequency curves are known for sub-daily rainfall heights (which are usually estimated in an indirect way by using Lambor's equations from daily data), while continuous daily series with a sufficient sample size are available. Attention is focused on modelling the sample frequency distributions of daily annual maximum rainfall. The obtained results are promising for further elaborations, concerning the use of STORAGE synthetic continuous rainfall data as input for a continuous rainfall-runoff approach, to be preferred with respect to classical event-based modelling.
Hybrid modelling of water resource recovery facilities: status and opportunities
Authors: Schneider Mariane Yvonne , Quaghebeur Ward , Borzooei Sina , Froemelt Andreas , Li Feiyi , Saagi Ramesh , Wade Matthew J. , Zhu Jun-Jie , Torfs Elena 
Published: April 2022

Abstract
Mathematical modelling is an indispensable tool to support water resource recovery facility (WRRF) operators and engineers with the ambition of creating a truly circular economy and assuring a sustainable future. Despite the successful application of mechanistic models in the water sector, they show some important limitations and do not fully profit from the increasing digitalisation of systems and processes. Recent advances in data-driven methods have provided options for harnessing the power of Industry 4.0, but they are often limited by the lack of interpretability and extrapolation capabilities. Hybrid modelling (HM) combines these two modelling paradigms and aims to leverage both the rapidly increasing volumes of data collected, as well as the continued pursuit of greater process understanding. Despite the potential of HM in a sector that is undergoing a significant digital and cultural transformation, the application of hybrid models remains vague. This article presents an overview of HM methodologies applied to WRRFs and aims to stimulate the wider adoption and development of HM. We also highlight challenges and research needs for HM design and architecture, good modelling practice, data assurance, and software compatibility. HM is a paradigm for WRRF modelling to transition towards a more resource-efficient, resilient, and sustainable future.
Methods of PARP-1 Determination and its Importance in Living
Organisms
Authors: Zielińska Zuzanna , Ołdak Łukasz , Gorodkiewicz Ewa 
Published: April 2022

Abstract:
PARP-1 is one of the 18 PARP enzymes that are involved in important processes at the
cellular level. The most important tasks of PARP-1 are to detect and repair DNA damage and to
prevent processes of apoptosis. By finding and using new strategies for marking and detecting the
activity of this protein, it is possible to identify more and more tasks in which it participates. In
pathological states, PARP-1 activity increases significantly. Since the 1980s, scientists have been
searching for and discussing substances that may inhibit PARP-1 activity and disrupt DNA damage
response pathways. In this way, unwanted cells could be destroyed. The paper presents a short
description of the methods used in the determination of PARP-1 by various research groups. A
critical approach to each of them was also made by pointing to the advantages and disadvantages of
the described analytical methods. The literature review contains information on methods useful for
PARP-1 determination, such as SPR, QCM, CL and FL, DPV, SDS-PAGE with MS, MALDI MS,
Western Blot, ELISA and ATR-FTIR spectroscopy. It also includes analysis of the results of
research on inhibitors that may be effective in the diagnosis and treatment of cancer and other
diseases.
Multimodal Medical Image Fusion Based on Intuitionistic Fuzzy Sets and
Weighted Activity Measure in NSST Domain
Authors: Kamarthi Vanitha , Satyanarayana Donthi , Ninjappa Giri Prasad Mahendra 
Published: April 2022

Background:
In the extraction of information from multimodality images, anatomical and functional image fusion became an effective tool in the applications of clinical imaging.

Objective:
A new approach to fuse anatomical and functional images that use the combination of activity measure and intuitionistic fuzzy sets in the NSST domain is presented.

Methods:
First, the high and low-frequency sub-images of source images are obtained by utilizing NSST decomposition, which represents them in multi-scale and multi-directions. Next, the high-fre-quency sub-images are applied to intuitionistic fuzzy sets, in which the fused coefficients are selected using an activity measure called fuzzy entropy.

Results:
The multiplication of weighted local energy and weighted sum modified Laplacian is used as an activity measure to fuse the low-frequency sub-images. At last, the reconstruction of the final fused image is done by applying the inverse NSST on the above-fused coefficients.

Conclusion:
The efficacy of the proposed fuzzy-based method is verifiable by five different modali-ties of anatomical and functional images. Both subjective and objective calculations showed better results than existing methods.