bluuebunny/crossref_metadata_2025_split huggingface dataset:
Talking methods, talking about methods: Invoking the transformative potential of social methods through animals, objects and how‐to instructions
Authors: Pottinger Laura , Barron Amy , Hall Sarah Marie , Ehgartner Ulrike , Browne Alison L. 
Published: March 2022

AbstractThis paper focuses on "talking" methods, noting their wide application across the social sciences, and identifies potential spaces for innovation in this field. Drawing on interview material from the Methods for Change project, we argue that researching methods requires creative approaches to talk. With research methods as our focus, we draw on data collected from online interviews with 36 academics, which aimed to explore the transformative potential of social science research methods. We make three contributions. First, we consider challenges and potentials for talking about methods and communicating the transformative potential of social science methods to diverse audiences. Second, we elaborate on the detail of doing talking methods, identifying potential spaces for innovation. Third, we suggest there is value in supplementing interviews with creative techniques when talking with and about method. We highlight three such techniques used in our project as a means of eliciting conversation about the transformative potential of methods: how‐to instructions; object interviewing; and methods as animals. The conceptual underpinnings, practical applications and obstacles encountered with each technique are discussed, including our own reflections on creative interviewing in a context where face‐to‐face research was restricted. In doing so, we respond to and advance recent debates about the need to talk more about the doing of talking methods. We argue that academics need to articulate why methods matter in creating change to global challenges, and that creative techniques can play a pivotal role.
Efficacy and safety of preoperative embolization in carotid body tumor treatment: A propensity score matching retrospective cohort study
Authors: Wu Zhaoyu , Qiu Peng , Pu Hongji , Ye Kaichuang , Liu Guang , Li Weimin , Liu Xiaobing , Yin Minyi , Jiang Mier , Qin Jinbao , Lu Xinwu , Zhao Zhen 
Published: March 2022

AbstractBackgroundTo assess the efficacy and safety of preoperative embolization (PE) in patients with carotid body tumor (CBTs).MethodsIn a single‐center retrospective cohort study, 127 patients underwent surgical resection of CBTs from January 2003 to December 2019. One‐to‐one propensity score matching was conducted between patients with or without PE.ResultsThirty‐two (25.2%) patients received PE. After propensity score matching, no statistically significant differences were found in the baseline characteristics of 28 patients in each group. Compared with NPE group, operative time and estimated blood loss (EBL) were significantly reduced in the PE group. The incidence of stroke, perioperative complications, intraoperative blood transfusion, vascular reconstruction, hospital stay, tumor recurrence, and all‐cause mortality were not different between the PE and NPE group.ConclusionsPreoperative embolization was efficient and safe with a reduction of intraoperative blood loss and operative time during CBT resection.
Protection and antibody levels 35 years after primary series with hepatitis B vaccine and response to a booster dose
Authors: Bruce Michael G. , Bruden Dana , Hurlburt Debby , Morris Julie , Bressler Sara , Thompson Gail , Lecy Danielle , Rudolph Karen , Bulkow Lisa , Hennessy Thomas , Simons Brenna C. , Weng Mark K. , Nelson Noele , McMahon Brian J. 
Published: March 2022

Abstract

Background and Aims
The duration of protection from hepatitis B vaccination in children and adults is not known. In 1981, we used three doses of plasma‐derived hepatitis B vaccine to immunize a cohort of 1578 Alaska Native adults and children from 15 Alaska communities who were ≥6 months old.

Approach and Results
We tested persons for antibody to hepatitis B surface antigen (anti‐HBs) levels 35 years after receiving the primary series. Those with levels <10 mIU/ml received one booster dose of recombinant hepatitis B vaccine 2–4 weeks later and were then evaluated on the basis of anti‐HBs measurements 30 days postbooster. Among the 320 recruited, 112 persons had not participated in the 22‐ or 30‐year follow‐up study (group 1), and 208 persons had participated but were not given an HBV booster dose (group 2). Among the 112 persons in group 1 who responded to the original primary series, 53 (47.3%) had an anti‐HBs level ≥10 mIU/ml. Among group 1, 73.7% (28 of 38) of persons available for a booster dose responded to it with an anti‐HBs level ≥10 mIU/ml at 30 days. Initial anti‐HBs level after the primary series was correlated with higher anti‐HBs levels at 35 years. Among 8 persons who tested positive for antibody to hepatitis B core antigen, none tested positive for HBsAg or HBV DNA.

Conclusions
Based on anti‐HBs level ≥10 mIU/ml at 35 years and a 73.7% booster dose response, we estimate that 86% of participants had evidence of protection 35 years later. Booster doses are not needed in the general population at this time.
CSDL‐Net: An iterative network based on compressed sensing and deep learning
Authors: Bian Cong Chao , Cao Ning , Mao Ming He 
Published: March 2022

AbstractCompressed sensing magnetic resonance imaging (CS‐MRI), as a traditional model‐based method, provides a theoretical framework for MRI reconstruction. The problems with traditional CS‐MRI reconstruction are solved by iterative numerical solvers, which have expensive computational cost and are long time consuming, limiting its application in MRI reconstruction. At present, deep learning (DL) as a new data‐driven method has made rapid progress in the field of computer vision and image processing. In this article, we apply DL network model based on CS (CSDL‐Net), a DL method with compressed sensing thory, for MR image reconstruction to generate high‐quality images in a short time with a lower sampling rate. It includes three blocks: data fusion block, image reconstruction block, and image fusion block. We utilize single‐coil part from FastMRI dataset, a widely known public database. Results show that the validation set. The average peak signal‐to‐noise ratio (PSNR) was 32.31 dB, and the structural similarity (SSIM) was 0.729, with a processing time of 1.21 s on the graphics processing unit (GPU). Therefore, the proposed method has a shorter reconstruction time and better image quality than the existing methods.
Oxidative stress in patients with asthma and its relation to uncontrolled asthma
Authors: Ammar Mariam , Bahloul Najla , Amri Oumayma , Omri Ribh , Ghozzi Hanene , Kammoun Samy , Zeghal Khaled , Ben Mahmoud Lobna 
Published: March 2022

AbstractThis study aims to evaluate markers of oxidative stress in Tunisian asthmatic patients and investigate whether their markers are correlated with uncontrolled asthma.This prospective cohort study was conducted on 48 healthy subjects and 60 patients with asthma (34 patients with controlled asthma and 26 patients with uncontrolled asthma). The levels of malondialdehyde (MDA), advanced oxidation protein products (AOPP), and glutathione (GSH), as well as the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD), were estimated in plasma by spectrophotometry.Asthmatic patients have significantly higher plasmatic levels of MDA and AOPP than healthy controls (p < 0.001). Lower GSH level and GPx activity were found in patients with asthma compared to controls (p < 0.001). In contrast, higher SOD activity was noted in asthmatic patients (p < 0.001).The comparison among the patients with controlled asthma and uncontrolled asthma revealed increased MDA and AOPP levels and SOD activity (p < 0.001) as well as a decreased GSH level and GPx activity (p = 0.004, p = 0.019) in patients with uncontrolled asthma. Spirometry level was significantly correlated with SOD activity (r = 0.447; p = 0.010), whereas no significant correlations were found with the other parameters (MDA, AOPP, GSH, and GPx).Asthmatic patients, especially those with uncontrolled asthma, suffer a high degree of reactive oxygen species (ROS) formation causing considerable oxidative stress. Increased MDA level and SOD activity and reduced GPx activity were predictors of poorly controlled asthma.
Screening and identification of biomarkers associated with the immune infiltration of intracerebral hemorrhage
Authors: Guo Hao , Zhang Yanjun , Hu Zhanfei , Wang Li , Du Hongyin 
Published: March 2022

AbstractBackgroundRecent studies showed that inflammation and immunity might play essential roles in the progression of intracerebral hemorrhage (ICH). However, the underlying mechanisms for changes at the cellular and molecular levels after ICH remain unclear.MethodsWe downloaded the microarray dataset of ICH from the Gene Expression Omnibus (GEO) database. The differential expression gene analysis was obtained by weighted gene co‐expression network analysis (WGCNA). We got the hub genes and performed the biological functions and signaling pathways of these genes by Metascape. GSVA algorithm was used to evaluate the potential physical function of time‐varying ICH samples. We used single‐sample gene set enrichment analysis (ssGSEA) to assess the immune signatures infiltration and analyzed the correlation between hub genes and immune signatures.ResultsThe data sets of all 22 ICH samples in GSE125512 were examined by the WGCNA R package. We finally screened five hub genes (GAPDH, PF4, SELP, APP, and PPBP) in the royal blue module. Metascape analysis displayed the biological processes related to inflammation and immunology. Cell adhesion molecule binding, myeloid leukocyte activation, CXCR chemokine receptor binding, and regulation of cytokine production were the most enriched pathophysiological process. The immune signatures infiltration analyses showed that ICH patients’ early and late samples had different activity and abundance of immune‐related cells and types.ConclusionsGAPDH, PF4, SELP, APP, and PPBP are identified as potential biomarkers for predicting the progression of ICH. This study may help us better understand the immunologic mechanism and shed new light on the promising approaches of immunotherapy for ICH patients.
Modeling of Pharmacokinetic Profiles of Insulin Aspart and Biphasic Insulin Aspart 30/70
Authors: Kulesh Victoria S. , Drai Roman V. , Zinnatulina Bella R. , Makarenko Igor E. , Pilyus Fedor G. , Khokhlov Alexander L. 
Published: March 2022

AbstractThis study includes modeling and simulation of insulin aspart pharmacokinetics (PK). The authors used PK data of biosimilar insulins—insulin aspart and biphasic insulin aspart 30/70—to develop a predictive population PK model for the insulins. The model was built via Monolix software, taking into account the weight‐based dosing and the dose and body‐weight effects on the parameters. The model‐based simulations were performed using the R package mlxR for various administered doses and various ratios of insulin aspart forms for a better understanding of the insulin behavior. The optimal model was a 1‐compartment model with a combination of zero‐ and first‐order absorptions, with absorption lag for the soluble form of insulin aspart and first‐order absorption for the insulin aspart protamine suspension. The assumption of identical behavior of 2 insulins at the distribution and elimination phases was made. The developed PK model was fitted successfully to the experimental data, and all fitted parameters displayed a moderate coefficient of variation. The PK model allows us to predict PK profiles for various doses and formulations of insulin aspart and can be used to improve the accuracy, safety, and ethics of novel clinical trials of insulin.
A negative feedback loop between fibroadipogenic progenitors and muscle fibres involving endothelin promotes human muscle fibrosis
Authors: Bensalah Mona , Muraine Laura , Boulinguiez Alexis , Giordani Lorenzo , Albert Victorine , Ythier Victor , Dhiab Jamila , Oliver Alison , Hanique Valentine , Gidaro Teresa , Perié Sophie , Lacau St‐Guily Jean , Corneau Aurélien , Butler‐Browne Gillian , Bigot Anne , Mouly Vincent , Negroni Elisa , Trollet Capucine 
Published: March 2022

AbstractBackgroundFibrosis is defined as an excessive accumulation of extracellular matrix (ECM) components. Many organs are subjected to fibrosis including the lung, liver, heart, skin, kidney, and muscle. Muscle fibrosis occurs in response to trauma, aging, or dystrophies and impairs muscle function. Fibrosis represents a hurdle for the treatment of human muscular dystrophies. While data on the mechanisms of fibrosis have mostly been investigated in mice, dystrophic mouse models often do not recapitulate fibrosis as observed in human patients. Consequently, the cellular and molecular mechanisms that lead to fibrosis in human muscle still need to be identified.MethodsCombining mass cytometry, transcriptome profiling, in vitro co‐culture experiments, and in vivo transplantation in immunodeficient mice, we investigated the role and nature of nonmyogenic cells (fibroadipogenic progenitors, FAPs) from human fibrotic muscles of healthy individuals (FibMCT) and individuals with oculopharyngeal muscular dystrophy (OPMD; FibMOP), as compared with nonmyogenic cells from human nonfibrotic muscle (MCT).ResultsWe found that the proliferation rate of FAPs from fibrotic muscle is 3–4 times higher than those of FAPs from nonfibrotic muscle (population doubling per day: MCT 0.2 ± 0.1, FibMCT 0.7 ± 0.1, and FibMOP 0.8 ± 0.3). When cocultured with muscle cells, FAPs from fibrotic muscle impair the fusion index unlike MCT FAPs (myoblasts alone 57.3 ± 11.1%, coculture with MCT 43.1 ± 8.9%, with FibMCT 31.7 ± 8.2%, and with FibMOP 36.06 ± 10.29%). We also observed an increased proliferation of FAPs from fibrotic muscles in these co‐cultures in differentiation conditions (FibMCT +17.4%, P < 0.01 and FibMOP +15.1%, P < 0.01). This effect is likely linked to the increased activation of the canonical TGFβ‐SMAD pathway in FAPs from fibrotic muscles evidenced by pSMAD3 immunostaining (P < 0.05). In addition to the profibrogenic TGFβ pathway, we identified endothelin as a new actor implicated in the altered cross‐talk between muscle cells and fibrotic FAPs, confirmed by an improvement of the fusion index in the presence of bosentan, an endothelin receptor antagonist (from 33.8 ± 10.9% to 52.9 ± 10.1%, P < 0.05).ConclusionsOur data demonstrate the key role of FAPs and their cross‐talk with muscle cells through a paracrine signalling pathway in fibrosis of human skeletal muscle and identify endothelin as a new druggable target to counteract human muscle fibrosis.
Synthesis of mesoporous Pt@KIT−6/SAPO−11 VIA in situ encapsulation to catalyze the decarboxylation of oleic acid to C8–C17 alkanes
Authors: Liu Xiaolong , Ma Jingye , Wang Minxin , Yuan Hong 
Published: March 2022

AbstractBACKGROUNDA series of mesoporous Pt@KIT−6/SAPO−11 catalysts with SiO2:Al2O3 molar ratios of 0.3, 0.4, or 0.5 and Pt loadings of 0.4, 0.8, or 1.2 wt% were prepared via an in situ encapsulation process using a PEO − PPO − PEO triblock copolymer (EO20PO70EO20, P123) as the mes. China 750001 oporous template and KIT−6 molecular sieves as the silicon source.RESULTSThese catalysts were characterized by transmission electron microscopy (TEM), specific surface area analysis, and pyridine–infrared spectroscopy (Py − IR). The results showed that Pt nanoparticles with particle sizes of 3–7 nm were uniformly dispersed on the supports. These materials also exhibited good mesoporous structures with specific surface areas of 138–197 m2/g and average pore sizes of 7.1–9.9 nm. The specimens having a SiO2:Al2O3 molar ratio of 0.4 also showed an orderly strip−shaped pore structure. The Py − IR results indicated that the catalysts had both Brønsted acid and Lewis acid sites, with total acid contents in the range of 77.8–131.1 μmol/g.CONCLUSIONThe decarboxylation of oleic acid using these materials under CO2 was examined. The catalyst with a SiO2:Al2O3 molar ratio of 0.4 and a Pt loading of 0.8 exhibited the best catalytic performance and a C8 − C17 alkanes yield as high as 85.1% at 340 °C by using a catalyst−to−oil mass ratio of 1:10 and a reaction time of 5 h. This performance was attributed to the ordered mesoporous structure of this catalyst along with its high surface area (173 m2/g), good crystallinity (92.8%), suitable acid content (98.6 μmol/g), and abundant Pt metal active sites. © 2022 Society of Chemical Industry (SCI).
Active removal of anionic azo dyes (MO,CR,EBT) from aqueous solution by potential adsorptive capacity of zinc oxide quantum dots
Authors: Karim Samina , Ahmad Nisar , Hussain Dilshad , Mok Young Sun , Siddiqui Ghayas Uddin 
Published: March 2022

AbstractBACKGROUNDOrganic dyes are extremely hazardous waste toxins because they can harm all living organisms as well as the environment. Among them, azo dyes are the most harmful, containing a non‐degradable azo group (NN); these dyes are highly hazardous for both humans and animals, even in low concentration. This research study aims to remove hazardous anionic azo dyes from aqueous solutions by a simple adsorption method using zinc oxide quantum dots (ZnO QDs) synthesized by a facile precipitation method.RESULTSAs synthesized, ZnO QDs were characterized by transmittance electron microscopy, atomic force microscopy, X‐ray powder diffraction, Fourier transform infrared spectroscopy, zeta potential and Brunauer–Emmett–Teller. The azo dyes Methyl Orange (MO), Congo Red (CR) and Eriochrome Black‐T (EBT) have been removed actively from an aqueous solution using the high adsorption characteristic of ZnO QDs. The results demonstrated that the adsorbent was efficient even at a low concentration at 0.05 g L−1. The maximum adsorption capacity of ZnO QDs at 27 ± 1 °C was 102.9, 124.3 and 60 mg L−1for MO, CR and EBT, respectively, and all these results have been observed in just 30 min. A pseudo‐second‐order and Langmuir isotherm model satisfactorily suited the dye adsorption data and, up to three cycles, the ZnO QDs demonstrated outstanding reusability.CONCLUSIONZnO QDs showed not only efficient removal of individual dyes but also simultaneous removal of azo dyes from mixture solution and proved to be an effective, eco‐friendly, low‐cost adsorbent for dye decontamination in wastewater. © 2022 Society of Chemical Industry (SCI).
Design, synthesis, and fungicidal activities of novel ethylenediamine bridged thiazole derivatives containing oxime ether or oxime ester moieties
Authors: Tian Xue‐Rong , Peng Xing‐Jie , Zhao Tong‐Tong , Bian Qiang , Zhao Wei‐Guang 
Published: March 2022

AbstractTo study the influence of changing the piperidine ring on the fungicidal activity, at the same time based on the successful experience of opening the oxazoline ring, a series of novel ethylenediamine bridged thiazole derivatives containing oxime ether and oxime ester moieties were designed, synthesized, and first evaluated for their fungicidal activities against phytophthora capsaicae in vitro. The bioassays results showed that the target compounds possessed moderate fungicidal activities against phytophtho‐ra capsaicae. Compound 28b showed 53.6% fungicidal activity at 50 μg/ml. Oxime ether and oxime ester derivatives showed the similar level of activities. However, the activities of these compounds were lower than that of oxathiapiprolin, suggesting the piperidine ring was essential to maintain the fungicidal activities of these compounds. For the oxime ether derivatives, 16b, 22a, 25b, and 28b, the substituents on ethylenediamine have certain influence on the activity. Increasing the chain length of substituents is beneficial to the fungicidal activity. Meanwhile, these compounds were tested their fungicidal activities against other 9 fungi, and these compounds showed broad spectrum fungicidal activities. Some compounds exhibited more than 70% fungicial activities against specific fungi.
Flaxseed‐derived peptides ameliorate hepatic cholesterol metabolism in Sprague–Dawley rats fed a high‐cholesterol and high‐fat diet
Authors: Yuan Xingyu , Bao Xiaolan , Liu Xiaojing , Li Xuexin 
Published: March 2022

AbstractBACKGROUNDPlant peptides have been reported to have cholesterol‐lowering activities. Previous research has found that ≤1 kDa flaxseed peptide (FP5) reduces cholesterol absorption and synthesis in vitro. In this research, we investigated the cholesterol‐lowering activity of FP5 in Sprague–Dawley (SD) rats fed a high‐cholesterol and high‐fat diet. In addition, amino acid sequences of FP5 were determined by high‐performance liquid chromatography–electrospray ionization–Orbitrap mass spectrometry.RESULTSFP5 supplement significantly decreased the serum and hepatic cholesterol levels and modulated the hepatic gene and protein expression of cholesterol metabolism‐related enzymes or regulators (3‐hydroxy‐3‐methylglutaryl coenzyme A reductase, Low‐Density Lipoprotein Receptor (LDLR), Cholesterol 7 α‐hydroxylase, Niemann–Pick C1‐like 1, ATP‐binding cassette transporters G5 and G8). Eleven peptides were identified from FP5. These peptides were characterized as hydrophobic amino acids such as leucine (L), proline (P), glycine (G), isoleucine (I) and continuous sequences, including LP, LL, LG and II, with low molecular weights.CONCLUSIONFP5 has a certain cholesterol‐lowering activity in SD rats fed a high‐cholesterol and high‐fat diet. The possible mechanism for ameliorating hepatic cholesterol metabolism of FP5 includes inhibiting hepatic cholesterol de novo synthesis, promoting the synthesis and excretion of bile acids, and inhibiting the reabsorption of bile acids during enterohepatic circulation. © 2022 Society of Chemical Industry.
Detection of near‐infrared autofluorescence from adrenal neoplasms: An initial experience
Authors: Noureldine Salem I. , Nazli Avci Seyma , Isiktas Gizem , Ergun Onuralp , Elshamy Mohammed , Berber Eren 
Published: March 2022

AbstractBackground and ObjectivesFluorescence from adrenal tumors can be detected with near‐infrared imaging after injection with indocyanine green. However, it is unknown if adrenal tumors exhibit autofluorescence. The aim of this study was to determine whether adrenal tumors emit near‐infrared autofluorescence (NIRAF).MethodsThis was a prospective study of patients who underwent minimally invasive adrenalectomy at a tertiary center. Intraoperative images were analyzed to detect NIRAF with a 750 nm camera. Descriptive and comparative statistical analyses were performed.ResultsTwenty‐five adrenalectomies were examined. Only 11 tumors (44%), that originated from the cortex exhibited autofluorescence. A contrast distinction between the tumor and retroperitoneum was observed in 23 patients, whereas a contrast distinction between the tumor and normal adrenocortical tissue was seen in 12 patients. The overall fluorescence intensity of adrenal tumors was found to be variable and ranging between 0.3 and 5.6 times that of the background tissue. Pheochromocytoma, malignancy and adrenal cyst did not demonstrate NIRAF.ConclusionThis is the first study to show that adrenocortical tissue can demonstrate NIRAF. The pattern of fluorescence was similar to that observed after indocyanine green injection in our historical experience. NIRAF has a potential to be used as an intraoperative optical adjunct during adrenalectomy.