bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery a novel hybrid with resveratrol and hans ester derivatives as activators induce autophagic cell death in tumoral NCI-H460 cells through production of ROS
Authors: Du yuting , Wang Hong-Liang , zhao San-Hu , zhang Xiao-Juan 
Published: February 2022

Abstract
Based on the integrated fragment-based drug design, synthesis, and in vivo evaluations, a series of novel resveratrol and hans ester (ReHa) derivatives are discovered as autophagic death inducer. Compound ReHa-2 is the most potent inducer with IC50 values as low as 9.9 mM compare with the molecule (NIP) in autophagic cell death. Compound ReHa-2 lead to autophagic cells death instead of necrosis or apoptosis in human NCI-H460 cells. Mechanistic study uncovers that ReHa-2 is capable of increasing protein LC3-II (a marker of autophagy) and reducing p62 in a time- and dose-dependent manner. Furthermore, ReHa-2 can activate MAPKs and Akt signal pathway. In addition, we identified that ReHa-2 triggered more ROS generation compare with NIP in NCI-H460 cells. Noticeably, the cytotoxicity induced by ReHa-2 against NCI-H460 cells can be significantly revised by pretreatment of the cells with the CAT (a specific scavenger of H2O2) and DTT (a sulfhydrylcontaining nucleophile for quenching ROS), suggesting that the ROS (mainly including H2O2) induced by ReHa-2 is responsible for its cytotoxicity against NCI-H460 cells. Our results suggest that ReHa-2 displays potent activators by inducing autophagic cell death through production of ROS.
Iron Isotopes Constrain Sub-Seafloor Hydrothermal Processes at the TAG Active Sulfide Mound, 26°N Mid-Atlantic Ridge
Authors: Sahlström Fredrik , Troll Valentin , Palinkaš Sabina Strmić , Kooijman Ellen , Zheng Xin-Yuan 
Published: February 2022

Abstract
Sub-seafloor hydrothermal processes along volcanically active plate boundaries are integral to the formation of seafloor massive sulfide (SMS) deposits and to oceanic Fe cycling, yet their nature is poorly understood. Here we apply Fe isotope analysis to sulfides from the TAG mound and underlying stockwork, 26°N Mid-Atlantic Ridge, to trace hydrothermal processes inside an actively-forming SMS deposit in a sediment-free mid-ocean ridge setting. We show that data for recently formed chalcopyrite imply fluid–mound interactions cause small negative shifts (< -0.1‰) to the δ56Fe signature of dissolved Fe released from TAG into the North Atlantic Ocean. Texturally resolved pyrite, in turn, preserves a δ56Fe range from − 1.27 to + 0.56‰ that reflects contrasting modes of formation (fluid–seawater mixing vs. fluid conductive cooling) and variable degrees of progressive hydrothermal maturation during the > 20 kyr evolution of the TAG complex. The identified processes may explain Fe isotope variations found in fossil onshore sulfide deposits.
COVID-19 Associated Rhino-Orbito-Cerebral Mucormycosis : A Single Tertiary Care Center Experience of Imaging Findings in COVID-19 Associated Mucormycosis with A Special Focus on the Intracranial Manifestations of Mucormycosis.
Authors: Nair Megha Gireesan , Susheelkumar Shilpa Sankhe , M Gayatri Autkar 
Published: February 2022

Abstract
Context: The current COVID-19 pandemic and mucormycosis epidemic in India has made research on radiological findings of COVID-19 associated mucormycosis imperative.Aim: To describe the imaging findings in COVID-19 associated mucormycosis with a special focus on the intracranial manifestations.Materials and methods: MRI scans of all patients with laboratory proven mucormycosis and post COVID-19 status, over a period of two months, at an Indian Tertiary Care Referral Centre, were retrospectively reviewed and descriptive statistical analysis was carried out.Results: 58 patients (47 men and 11 women) were evaluated. Deranged blood glucose levels were observed in 81% of cases. Intracranial invasion was detected in 31 patients (53.4%). The most common finding in cases with intracranial invasion was pachymeningeal enhancement (90.3% i.e., 28/31). This was followed by infarcts (17/31 i.e., 55%), cavernous sinus thrombosis (11/58 i.e., 18.9%), fungal abscesses (11/31 i.e., 35.4%), and intracranial hemorrhage (5/31 i.e., 16.1% cases). Perineural spread was observed in 21.6% (11/51) cases.Orbital findings included extraconal fat and muscle involvement, intraconal involvement, orbital apicitis, optic neuritis, panophthalmitis, and orbital abscess formation in decreasing order of frequency. Cohen’s kappa coefficient of inter-rater reliability for optic nerve involvement and cavernous sinus thrombosis was 0.7. Cohen’s coefficient value for all other findings was 0.8-0.9.Conclusions: COVID-19 associated Rhinocerebral mucormycosis has a plethora of orbital and intracranial manifestations. MRI, with its superior soft-tissue resolution, is the imaging modality of choice to expedite the initial diagnosis, accurately map out disease extent, and for prompt identification and scrupulous management of its complications.
Relationship Between Workplace Bullying, Spirituality And Job Burnout In Pediatric Nurses: A Cross-Sectional Study
Authors: Fan Ying , Cao Mi , Zhou Yumei , Duan Peng , Xing Limin 
Published: February 2022

Abstract
Background: Workplace bullying threatens public health as a serious social problem globally. The nursing field is one of the hardest hit areas where bullying is breeding. The purpose of this study was to investigate the relationships and pathways between workplace bullying, workplace spirituality and job burnout in Chinese pediatric nurses. Methods: The sample of this cross-sectional study consists of 402 pediatric nurses who worked at six hospitals in Hubei Province, China. The data were collected using a sociodemographic data questionnaire, Negative Acts Questionnaire-Revised, Maslach Burnout Inventory-General Survey and Workplace Spirituality Scale. The model was tested using path analysis techniques within structural equation modeling.Result: The research data supported the proposed model. Workplace bullying had positive and direct effects on the burnout of pediatric nurses. Workplace spirituality partially mediated the relationship between workplace bullying and burnout.Conclusions: Workplace spirituality plays an important role in correctly recognizing and evaluating work stressors. Workplace spirituality may reduce the incidence of bullying and burnout in pediatric nurses. Nursing managers should first acknowledge the existence of workplace bullying. Next, they need to consider and cultivate the workplace spirituality of pediatric nurses, with the aim of creating a healthy working environment and ensuring the stability of the nursing team.
Quantitative Analysis of Retained Austenite in Nb Added Fe-based Alloy
Authors: Ko Kwang Kyu , Jang Jin Ho , Tiwari Saurabh , Bae Hyo Ju , Sung Hyo Kyung , Kim Jung Gi , Seol Jae Bok 
Published: February 2022

Abstract
Use of Pipe lines for long-distance transportation of crude oil, natural gas etc., increasing and have pivotal importance in recent times. To improve transport efficiency through increased pressure and improved laying efficiency through reduced diameter and weight of line pipes, high specific strength plays a crucial role. TRIP-based high-strength and high-ductility alloys comprise a mixture of ferrite, bainite, and retained austenite that provide excellent mechanical properties such as dimensional stability, fatigue strength, and impact toughness. In this study, microstructure analysis performs using both Nital etching and LePera etching methods. At the time of Nital etching, it is difficult to distinctly observe second phase. However, using LePera etching conditions it is possible to distinctly measure M / A phase and ferrite matrix. The fraction measurement was done using OM and SEM images which gives similar results for the average volume fraction. Although it is possible to distinguish the M / A phase from the SEM image of the sample subjected to LePera etching, but using Nital etching it is impossible. Nital etching is good at specific phase analysis than LePera etching through the SEM images.
Are Hyperlipidemia and Lipid-Lowering Drugs Risk Factors for Diabetic Peripheral Neuropathy?
Authors: Chang Kuo-Cheng , Pai Yen-Wei , Lin Ching-Heng , Lee I-Te , Chang Ming-Hong 
Published: February 2022

Abstract
Aims
Previous studies showed conflicting relationship between hyperlipidemia, lipid-lowering therapy and diabetic peripheral neuropathy (DPN). As most of these works emerges from the Western and Australian countries, our study aims to investigate whether hyperlipidemia or lipid-lowering drugs is associated with the risk of DPN in Taiwanese patients with type 2 diabetes (T2D).
Methods
A cross-sectional, hospital-based observation study in adults with T2D was conducted from January to October 2013. DPN was screened using the Michigan Neuropathy Screening Instrument. Diagnosis of hyperlipidemia was based on serum levels of lipid profiles. Patients taking lipid-lowering drugs were identified at enrollment.
Results
2,448 participants were enrolled, 524 (21.4%) of whom had DPN. Patients with DPN had lower plasma total cholesterol (TC; 185.6 ± 38.6mg/dL vs 193.4 ± 42.3mg/dL, P < 0.001) and low-density lipoprotein cholesterol levels (LDL-C; 114.6 ± 32.7mg/dL vs 119 ± 30.8mg/dL, P = 0.019). Multivariate analysis demonstrated that neither hyperlipidemia nor lipid-lowering drugs was associated with a risk of DPN. Subgroup analysis revealed that high TC, LDL-C, and statin or fibrate use were not associated with DPN occurrence.
Conclusion
Our results suggests that neither hyperlipidemia nor lipid-lowering medication was associated with DPN in adults with T2D. DPN is a multifactorial disease, and our findings indicate that lipid metabolism may play a minor role in its pathogenesis.
Risk Factors for Recurrence in Elderly Patients with Stage II Colorectal Cancer: A Multicenter Retrospective Study
Authors: Yagyu Takuki , Yamamoto Manabu , Tanio Akimitsu , Hara Kazushi , Sugezawa Ken , Uejima Chihiro , Kihara Kyoichi , Tatebe Shigeru , Kurisu Yasuro , Shibata Shunsuke , Yamamoto Toshio , Nishie Hiroshi , Shiota Setsujo , Saito Hiroaki , Naka Takuji , Sugamura Kenji , Katano Kuniyuki , Fujiwara Yoshiyuki 
Published: February 2022

Abstract
BackgroundAdjuvant chemotherapy for stageII colorectal cancer (CRC)is considered appropriate for patients with risk factorsfor recurrence, rather than for all patients uniformly. However, the risk factors for recurrence remain controversial, and there is limited information, especially for elderly patients.The Geriatric Nutritional Risk Index (GNRI) is widely used as a simple nutritional screening tool in the elderly and is associated with cancer prognosis and recurrence. This study aimed to investigate the risk factors for recurrence in the elderly with stageII CRC, focusing on the GNRI.MethodsWe enrolled 348elderly patients (≥ 75 years) with stageII CRC who underwent curative resection at the Department of Surgery, Tottori University and our 10 affiliated institutions.The patients were divided into GNRIhigh (≥93.465) and GNRIlow (<93.465) groups.ResultsThe GNRIlow group showed a significantly worse overall survival (OS), cancer-specific survival (CSS), and relapse-free survival (RFS) (P<0.001, P<0.001, and P<0.001, respectively).In a multivariate analysis, GNRIlow (hazard ratio [HR]:2.543, P<0.001), pathologic T4 stage (HR:2.380, P=0.001), and moderate to severe lymphatic or venous invasion (HR:2.194, P=0.001) were independent factors affecting RFS. By using these three factors to score the risk of recurrence from 0 to 3 points, the prognosis was significantly stratified in terms of OS, CSS, and RFS (P<0.001, P<0.001, and P<0.001, respectively). The recurrence rate for each score was as follows: 0 points,9.8%; 1 point, 22.0%; 2 points, 37.3%; and 3 points,61.9%.ConclusionsGNRIlow, pathologic T4 stage, and moderate to severe lymphatic or venous invasion are high-risk factors for recurrence in the elderly with stageII CRC. The scoring system using these three factors appropriately predicted their recurrence and outcome.
Surveillance of SARS-CoV-2 in the environment and animal samples of the Huanan Seafood Market
Authors: Gao George , Liu William , Liu Peipei , Lei Wenwen , Jia Zhiyuan , He Xiaozhou , Liu Lin-Lin , Shi Weifeng , Tan Yun , Zou Shumei , Zhao Xiang , Wong Gary , Wang Ji , Wang Feng , Wang Gang , Qin Kun , Gao Rongbao , Zhang Jie , Li Min , Xiao Wenling , Guo Yuanyuan , Xu Ziqian , Zhao Yingze , Song Jingdong , Zhang JIng , Zhen Wei , Zhou Wenting , Ye Beiwei , Song Juan , Yang Mengjie , Zhou Weimin , Bi Yuhai , Cai Kun , Wang Dayan , Tan Wenjie , Han Jun , Xu Wenbo , Wu Guizhen 
Published: February 2022

Abstract
Emerging in December 2019, coronavirus disease 2019 (COVID-19) eventually became a pandemic and has posed a tremendous threat to global public health. However, the origins of SARS-CoV-2, the causative agent of COVID-19, remain to be determined. It has reported that a certain number of the early case clusters had a contact history with Huanan Seafood Market. Therefore, surveillance of SARS-CoV-2 within the market is of vital importance. Herein, we presented the SARS-CoV-2 detection results of 1380 samples collected from the environment and the animals within the market in early 2020. By SARS-CoV-2-specific RT-qPCR, 73 environmental samples tested positive for SARS-CoV-2 and three live viruses were successfully isolated. The viruses from the market shared nucleotide identity of 99.980% to 99.993% with the human isolate HCoV/Wuhan/IVDC-HB-01. In contrast, no virus was detected in the animal swabs covering 18 species of animals in the market. The SARS-COV-2 nucleic acids in the positive environmental samples showed significant correlation of abundance of Homo sapiens with SARS-CoV-2. In summary, this study provided convincing evidence of the prevalence of SARS-CoV-2 in the Huanan Seafood Market during the early stage of COVID-19 outbreak.
Immunological Characteristics of MAV/06 strain of Varicella-Zoster Virus Vaccine in an Animal model
Authors: Shin Duckhyang , Shin Younchul , Kim Eunmi , Nam Hyojung , Nan Haiyan , Lee Jaewoo 
Published: February 2022

Abstract
Background: Varicella-zoster virus (VZV) is a pathogen that causes chickenpox and shingles in humans. Different types of the varicella vaccines derived from the Oka and MAV/06 strains are commercially available worldwide. Although the MAV/06 vaccine was introduced in 1990s, little was known about immunological characteristics. Results: Here, we evaluated B and T cell immune response in animals inoculated with the Oka and MAV/06 vaccines as well as a new formulation of the MAV/06 vaccine. A variety of test methods were applied to evaluate T and B cell immune response. Plaque reduction neutralization test (PRNT) and fluorescent antibody to membrane antigen (FAMA) assay were conducted to measure the MAV/06 vaccine-induced antibody activity against various VZVs. Glycoprotein enzyme-linked immunosorbent assay (gpELISA) was used to compare the degree of the antibody responses induced by the two available commercial VZV vaccines and the MAV/06 vaccine. Interferon-gamma enzyme-linked immunosorbent spot (IFN-γ ELISpot) assays and cytokine bead array (CBA) assays were conducted to investigate T cell immune responses. Antibodies induced by MAV/06 vaccination showed immunogenicity against a variety of varicella-zoster virus and cross-reactivity among the virus clades. Conclusions: It is indicating the similarity of the antibody responses induced by commercial varicella vaccines and the MAV/06 vaccine. Moreover, VZV-specific T cell immune response from MAV/06 vaccination was increased via Th1 cell response. MAV/06 varicella vaccine induced both humoral and cellular immune response via Th1 cell mediated response.
Identification of molecular pathways and prognostic biomarkers with survival analysis associated with UV-mediated skin cancer by bioinformatics approaches
Authors: Rahman Md Habibur , Hossain Arju , Ahsan Asif , Hasan Imran , Sohel Md , Khan Arif , Miah Md Sipon 
Published: February 2022

Abstract
Skin cancer (SC) referred to as cutaneous carcinoma is a serious public health concern on a global scale particularly for those with fair skin. Ultraviolet (UV) radiation causes skin cancer, but the exact mechanism by which occurs and the most effective methods of intervention to prevent it are yet unknown. Using bioinformatic approaches several biomarkers have been identified to determine the severity of skin cancer. This study will use bioinformatics and pharmacology approaches to discover potential biomarkers of SC for early diagnosis, prevention of disease, and therapeutic target identification.
This study compared gene expression and protein levels in UV-mediated cultured keratinocytes and adjacent normal skin tissue using RNA sequencing data from the NCBI-GEO database. Then we employed GO and signalling pathway database, selection of hub genes from protein-protein interaction (PPI) network, survival and expression profile, and gene regulatory network analysis to screen potential clinical biomarkers.
In the study, we identified 32 shared differentially expressed genes (DEGs) including 19 upregulated genes and 13 downregulated genes by analyzing three different subsets of the GSE85443 dataset. Skin cancer development is related to control of several DEGs through cyclin-dependent protein serine/threonine kinase activity, cell cycle regulation and activation of the NIMA kinase pathways. The cytohubba plugin in Cytoscape identified twelve hub genes from PPI; among these three DEGs namely, AURKA, CDK4, and PLK1 were shown to be significantly associated with survival (p $<$ 0.05) and highly expressed in SC tissues. Transcriptional, post-transcriptional, and protein-chemical also indicates the hub genes bound to several molecules.

Further investigation and clinical experiments will be needed to evaluate the expression of these identified biomarkers regarding the prognosis of SC patients.
Association of metabolic syndrome with TyG index and TyG-related parameters in an urban Chinese population: a 15-year prospective study
Authors: Zhang Xin , Jia Shanshan , zhang zhipeng , ye runyu , yang xiangyu , chen xiaoping 
Published: February 2022

Abstract
Background: The metabolic syndrome(Mets) is a multiplex risk factor for atherosclerotic cardiovascular diseases．The aims of the study were to assess the association of the Mets with TyG index and TyG-related parameters in an urban Chinese population.Methods and Results: The data were collected in 1992 and then again in 2007 from the same group of 590 individuals (363 males and 227 females ) without Mets in 1992. During 15 years’ follow-up, TyG-waist to height ratio (TyG-WHtR) shows the largest AUC for MS detection (0.686) followed by TyG-waist circumference (TyG-WC) (0.660), TyG-waist-to-hip ratio (TyG-WHpR) (0.564), and TyG index (0.556) in all participants. Gender analysis revealed that TyG-WHtR and TyG-WC have the largest AUC in both genders. TyG-WHtR significantly predicted Mets in all participants, with an unadjusted odds ratio of 5.63 (95% CI: 3.23-9.83 P＜0.001). Associations remained significant after adjustment for smoking, drinking, physical exercise and components of Mets. Conclusions: TyG-WHtR might be a strong and independent predictor for Mets in all participants in an urban Chinese population. TyG-related markers that combine obesity markers with TyG index are superior to other parameters in identifying Mets in both genders.
Enhanced Structural, Optical, And Photocatalytic Activities Of Cuo/Tio2:Zro2 Catalysts For Degrading Methyl Orange (MO) Dye Under UV–Visible Irradiation.
Authors: BENSOUYAD Hanene , Bensaha Rabah 
Published: February 2022

Abstract
On latest years, an emergency requirement for clean methods to improvement of environment worth has attracted attention in several researches’ fields. The use of films on photocatalysis process is one of the most interesting alternatives due to its easily on separation of residual water and recovery of photocatalyst. In this study, CuO/TiO2:ZrO2 catalysts with various molar ratios of CuO were synthesized by sol-gel dip-coating process. The dip-coated thin films have been examined at different annealing temperatures 500, 800 and 1000 °C. The crystalline structures, morphology, constituent contents, optical and photocatalytic properties of the as-prepared catalysts were analyzed by X–ray diffraction (XRD), Raman spectroscopy, FT-IR spectra, UV-VIS, Photoluminescence spectroscopy (PL) techniques. X-ray diffraction (XRD) and Raman results indicated that an increase in annealing temperature from 500°C to 1000°C caused a transformation of crystallinity from anatase and brookite at 500°C to rutile and monoclinic CuO phases at 1000°C, accompanied by an increase in the particle sizes. The optical transmittance of the films, increased to over 90% in the visible region, indicate that copper loading decreases the band gap from 3.53 to 3.39eV. The PL experiments revealed that the recombination of photoinduced electrons and holes is hindered by copper adding effectively, which is favorable for photocatalytic performance. 5%CuO/TiO2:ZrO2 exhibits the highest photocatalytic activity and the degradation degree of methyl orange (MO) reaches more than 99% after 3h. Photocatalytic experiments confirmed that our catalysts displayed highly efficient and durable activity for the photodegradation of methyl orange under visible light.
Noise self-cancelling metrology using twisted light
Authors: Munjal Pooja , Chaudhary Komal , Singh Kamal 
Published: February 2022

Abstract
Noise fundamentally limits the ultimate optical precision. Here, we unveil a real-time noise self-cancelling metrology by exploiting the unique spiral phase of light possessing orbital angular momentum (OAM). With a compact twisted interferometer and a novel measurement scheme exploiting an ensemble of N phase-orthogonal single-pixel intensity pairs on petals of the daisy flower-like interference pattern, we cancel various noises (intensity noise, interferometer noise, and detector noise) by three orders of magnitude, enabling detection of a weak input signal, in excellent agreement with our theoretical analysis. Remarkably, our approach allows detection of an aperiodic, non-repetitive dynamic event masked by 100x larger noise background faithfully with picoscale resolution, which is otherwise undetectable. Because no upper limit on the quantum numbers of OAM state exists, in principle, the noise cancellation scales up indefinitely. These results open promising ways to enhance precision metrology, secure communication, and develop analogous ideas for twisted acoustic waves, electron beams, and matter waves.