bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased expression of pro‐inflammatory cytokines at the fetal–maternal interface in bovine pregnancies produced by cloning
Authors: Rutigliano Heloisa M. , Thomas Aaron J. , Umbaugh Janae J. , Wilhelm Amanda , Sessions Benjamin R. , Kaundal Rakesh , Duhan Naveen , Hicks Brady A. , Schlafer Donald H. , White Kenneth L. , Davies Christopher J. 
Published: January 2022

AbstractProblemA significant rate of spontaneous abortion is observed in cattle pregnancies produced by somatic cell nuclear transfer (SCNT). Major histocompatibility complex class I (MHC‐I) proteins are abnormally expressed on the surface of trophoblast cells from SCNT conceptuses.Method of studyMHC‐I homozygous compatible (n = 9), homozygous incompatible (n = 8), and heterozygous incompatible (n = 5) pregnancies were established by SCNT. Eight control pregnancies were established by artificial insemination. Uterine and trophoblast samples were collected on day 35 ±1 of pregnancy, the expression of immune‐related genes was examined by qPCR, and the expression of trophoblast microRNAs was assessed by sequencing.ResultsCompared to the control group, trophoblast from MHC‐I heterozygous incompatible pregnancies expressed increased levels of CD28, CTLA4, CXCL8, IFNG, IL1A, IL2, IL10, IL12B, TBX21, and TNF, while GNLY expression was downregulated. The MHC‐I homozygous incompatible treatment group expressed increased levels of IFNG, IL1A, and IL2 while the MHC‐I homozygous compatible group did not differentially express any genes compared to the control group. In the endometrium, relative to the control group, MHC‐I heterozygous incompatible pregnancies expressed increased levels of CD28, CTLA4, CXCL8, IFNG, IL10, IL12B, and TNF, while GATA3 expression was downregulated. The MHC‐I homozygous incompatible group expressed decreased amounts of CSF2 transcripts compared with the control group but did not have abnormal expression of any other immune‐related genes. MHC‐I incompatible pregnancies had 40 deregulated miRNAs compared to control pregnancies and 62 deregulated microRNAs compared to MHC‐I compatible pregnancies.ConclusionsMHC‐I compatibility between the dam and fetus prevented an exacerbated maternal immune response from being mounted against fetal antigens.
Fasciculoventricular pathways—A rare and innocent variant: A Retrospective study focusing on clinical and electrophysiologic characteristics
Authors: Gormel Suat , Yasar Salim 
Published: January 2022

AbstractBackgroundFasciculoventricular pathways (FVPs) are variants of pre‐excitation syndrome which were investigated insufficiently because of its rarity.ObjectiveThis report aimed to represent one of the largest series of FVP, focusing on its clinical and electrophysiological properties.MethodsWe analyzed retrospectively 26 consecutive patients who underwent electrophysiological study (EPS) for FVP between January 1998 and June 2020.ResultsAmong 1437 patients with accessory pathways, 26 had FVP (1.80%). All the 26 patients (100%) were males, with a mean age of 22.15 ± 3.50 years (range, 20–34 years). In the baseline electrocardiograms of the patients with FVP, pre‐excitation and transitional zone were seen in leads V2–V4. During EPS procedures, normal AH interval and shortened HV interval were detected. All the patients had AH prolongation after atrial pacing due to atrioventricular (AV) nodal delay without change in pre‐excitation degree. Five of the FVP patients (19.2%) had extra accessory pathways, all of which were ablated successfully while the FVPs were followed clinically.ConclusionFasciculoventricular pathways are uncommon variants of pre‐excitation syndrome; therefore, they should be diagnosed correctly and followed up noninvasively to avoid damages.
National trends and outcomes of heart‐kidney transplantation using hepatitis C positive donors
Authors: Diaz‐Castrillon Carlos E. , Huckaby Lauren V. , Witer Lucas , Pope Nicolas H. , Katz Marc R. , Baliga Prabhakar K. , Kilic Arman 
Published: January 2022

AbstractBackgroundThis study evaluated the outcomes of combined heart‐kidney transplantation in the United States using hepatitis C positive (HCV+) donors.MethodsAdults undergoing combined heart‐kidney transplantation from 2015 to 2020 were identified in the United Network for Organ Sharing registry. Patients were stratified by donor HCV status. Kaplan‐Meier curves with multivariable Cox regression models were used for risk‐adjustment in a propensity‐matched cohort.ResultsA total of 950 patients underwent heart‐kidney transplantation of which 7.8% (n = 75) used HCV+ donors; 68% (n = 51) were viremic and 32% (n = 24) were non‐viremic donors. Unadjusted 1‐year recipient survival was similar between HCV+ versus HCV‐ donors (84% vs 88%, respectively; P = .33). Risk‐adjusted analysis in the propensity‐matched cohort showed HCV+ donor use did not confer increased risk of 1‐year mortality (hazard ratio .63, 95% CI .17‐2.32; P = .49). Sub‐group analysis showed viremic and non‐viremic HCV+ donors had similar 1‐year survival as well (84% vs 84%; P = .95).ConclusionsCompared with recipients of HCV‐ donor dual heart‐kidney transplants, recipients of HCV+ organs had comparable 1‐year survival and clinical outcomes after combined transplantation. Although future studies should evaluate other outcomes related to HCV+ donor use, this practice appears safe and should be expanded further in the heart‐kidney transplant population.
Bioactivity Evaluation of Leucas zeylanica and its Phytochemicals
Authors: Fui Lin Lee , Mohd Salleh Noor Aida Fazira , Abdullah Faizuan , Hashim Siti Ernieyanti 
Published: January 2022

Leucas zeylanica belongs to the Lamiaceae family has high medicinal value. The phytochemicals screening results showed that terpenoids, flavonoids, tannins, saponins, phenols and alkaloids are present in aerial part of L. zeylanica.Vacuum liquid chromatography (VLC) and column chromatography (CC) techniques were used in fractionation and purification of the crude extracts.
A Rudimentary X band CW-EPR Spectrometer Based on FPGA
Authors: Eow Wei Siang , Yap Yung Szen 
Published: January 2022

A rudimentary Electron Paramagnetic Resonance (EPR) spectrometer is design using a field programmable gate array (FPGA) equipped with two digital-to-analog (DAC) and two analog-to-digital (ADC) channels. The single stage heterodyne setup operates at X band frequencies and is used to detect EPR signals from 2,2-diphenyl-1-picrylhydrazyl (DPPH) in a loop-gap resonator. We design the loop gap resonator with 3 loops 2 gaps for high field homogeneity and moderate Q-factor. The resonator is coupled capacitively to the coaxial cable and is designed to have an unloaded resonant frequency of 8.856 GHz with a Q-factor of 646.0 when critically coupled. The loaded resonant frequency is reported to be 8.668 GHz with a Q-factor of 615.8. Using this setup, EPR signal is successfully detected at 311.4 mT and 8.688 GHz with an experimental g-factor of 1.99450.0012, which is very near to the standard value for DPPH.
Multi-objective regression modeling for natural gas prediction with ridge regression and CMARS
Authors: Ozmen Ayse 
Published: January 2022

Residential customers are the main users generally need a great quantity of natural gas in distribution systems, especially, in the wintry weather season since it is particularly consumed for cooking and space heating. Hence, it ought to be non-interruptible. Since distribution systems have a restricted ability for supply, reasonable planning and prediction through the whole year, especially in winter seasons, have emerged as vital. The Ridge Regression (RR) is formulated mainly to decrease collinearity results through shrinking the regression coefficients and reducing the impact in the model of variables. Conic multivariate adaptive regression splines ((C)MARS) model is constructed as an effective choice for MARS by using inverse problems, statistical learning, and multi-objective optimization theories. In this approach, the model complexity is penalized in the structure of RR and it is constructed a relaxation by utilizing continuous optimization, called Conic Quadratic Programming (CQP). In this study, CMARS and RR are applied to obtain forecasts of residential natural gas demand for local distribution companies (LDCs) that require short-term forecasts, and the model performances are compared by using some criteria. Here, our analysis shows that CMARS models outperform RR models. For one-day-ahead forecasts, CMARS yields a MAPE of about 4.8%, while the same value under RR reaches 8.5%. As the forecast horizon increases, it can be seen that the performance of the methods becomes worse, and for a forecast one week ahead, the MAPE values for CMARS and RR are 9.9% and 18.3%, respectively.
Approaching Things that Trigger Things: A Review of Three Shifts in the Character of Things and Their Implications for Design
Authors: Wiberg Mikael 
Published: January 2022

Abstract
In this article I focus on the changing character of things. In doing so, I review and draw together a set of existing sources, and emerging strands and conceptions of design, to discuss three fundamental shifts that have profoundly changed the character of the things we surround ourselves with, and I discuss their implications for design. I contribute to existing design research by bringing these strands together, and by making this accessible in the form of the three shifts.
Materializing the Agency of Design in Innovation
Practices
Authors: Neubauer Ruth M. 
Published: January 2022

Abstract
This article critically reviews how we think about design in technology and service innovation. Human-centered design has emerged in this science-driven field as a way to include the interests of humans and their needs in production processes. As such, design has a considerable effect on the development of new technologies and services. Making visible the agency of design in these practices thus is of immense importance. A gap remains in the ability of current concepts of design to visualize and conceptualize design agency. Therefore, drawing on concepts of materiality in design and practice, this article proposes a framework that makes design agency visible.
Merging existing practices with new ones: the adjustment of organizational routines to using cancer patient pathways in primary healthcare
Authors: Fjällström Petter , Coe Anna-Britt , Lilja Mikael , Hajdarevic Senada 
Published: January 2022

Abstract
Background
The introduction of new tools can bring unintended consequences for organizational routines. Cancer Patient Pathways (CPP) were introduced into the Swedish healthcare system in 2015 to shorten time to diagnosis and treatment. Primary healthcare (PHC) plays a central role since cancer diagnosis often begins in PHC units. Our study aimed to understand how PHC units adjusted organizational routines to utilizing CPPs.

Method
Six PHC units of varied size from both urban and rural areas in northern Sweden were included. Grounded theory method was used to collect and analyse group interviews at each unit. Nine group interviews with nurses and physicians, for a total of 41 participants, were performed between March and November 2019. The interviews focused on CPPs as tools, the PHC units’ routines and providers’ experiences with using CPPs in their daily work.

Results
Our analysis captured how PHC units adjusted organizational routines to utilizing CPPs by fusing existing practices with new practices to offer better quality of care. Specifically, three overarching organizational routines within the PHC units were identified. First, Manoeuvring diverse patient needs with easier patient flow, the PHC units handled the diverse needs of the population while simultaneously drawing upon CPPs to ease the patient flow within the healthcare system. Second, (Dis) integrating internal know-how, the PHC units drew upon internal competence even when PHC know-how was not taken into account by those driving the CPP initiative. Third, Coping with unequal relationships toward secondary care, the PHC units dealt with being in an unequal position while adopting CPPs instead further decreased possibilities to influence decision-making between care-levels.

Conclusion
Adopting CPPs as a tool within PHC units brought various unintended consequences in organizational routines. Our study from northern Sweden illustrates that the PHC know-how needs to be integrated into the healthcare system to improve the use of new tools as CPP. Further, the relationships between different levels of care should be taken in account when introducing new tools for healthcare. Also, when adopting innovations, unintended consequences need to be further explored empirically in diverse healthcare contexts internationally in order to generate deeper knowledge in the research area.
A data quality assessment of the first four years of malaria reporting in the Senegal DHIS2, 2014–2017
Authors: Muhoza Pierre , Tine Roger , Faye Adama , Gaye Ibrahima , Zeger Scott L. , Diaw Abdoulaye , Gueye Alioune Badara , Kante Almamy Malick , Ruff Andrea , Marx Melissa A. 
Published: January 2022

Abstract
Background
As the global burden of malaria decreases, routine health information systems (RHIS) have become invaluable for monitoring progress towards elimination. The District Health Information System, version 2 (DHIS2) has been widely adopted across countries and is expected to increase the quality of reporting of RHIS. In this study, we evaluated the quality of reporting of key indicators of childhood malaria from January 2014 through December 2017, the first 4 years of DHIS2 implementation in Senegal.

Methods
Monthly data on the number of confirmed and suspected malaria cases as well as tests done were extracted from the Senegal DHIS2. Reporting completeness was measured as the number of monthly reports received divided by the expected number of reports in a given year. Completeness of indicator data was measured as the percentage of non-missing indicator values. We used a quasi-Poisson model with natural cubic spline terms of month of reporting to impute values missing at the facility level. We used the imputed values to take into account the percentage of malaria cases that were missed due to lack of reporting. Consistency was measured as the absence of moderate and extreme outliers, internal consistency between related indicators, and consistency of indicators over time.

Results
In contrast to public facilities of which 92.7% reported data in the DHIS2 system during the study period, only 15.3% of the private facilities used the reporting system. At the national level, completeness of facility reporting increased from 84.5% in 2014 to 97.5% in 2017. The percentage of expected malaria cases reported increased from 76.5% in 2014 to 94.7% in 2017. Over the study period, the percentage of malaria cases reported across all districts was on average 7.5% higher (P < 0.01) during the rainy season relative to the dry season. Reporting completeness rates were lower among hospitals compared to health centers and health posts. The incidence of moderate and extreme outlier values was 5.2 and 2.3%, respectively. The number of confirmed malaria cases increased by 15% whereas the numbers of suspected cases and tests conducted more than doubled from 2014 to 2017 likely due to a policy shift towards universal testing of pediatric febrile cases.

Conclusions
The quality of reporting for malaria indicators in the Senegal DHIS2 has improved over time and the data are suitable for use to monitor progress in malaria programs, with an understanding of their limitations. Senegalese health authorities should maintain the focus on broader adoption of DHIS2 reporting by private facilities, the sustainability of district-level data quality reviews, facility-level supervision and feedback mechanisms at all levels of the health system.
A public health value-based healthcare paradigm for HIV
Authors: Vermeersch Sebastian , Demeester Rémy P. , Ausselet Nathalie , Callens Steven , De Munter Paul , Florence Eric , Goffard Jean-Christophe , Henrard Sophie , Lacor Patrick , Messiaen Peter , Libois Agnès , Seyler Lucie , Uurlings Françoise , Vandecasteele Stefaan J. , Van Wijngaerden Eric , Yombi Jean-Cyr , Annemans Lieven , De Wit Stéphane 
Published: January 2022

Abstract
Background
HIV patients face considerable acute and chronic healthcare needs and battling the HIV epidemic remains of the utmost importance. By focusing on health outcomes in relation to the cost of care, value-based healthcare (VBHC) proposes a strategy to optimize quality of care and cost-efficiency. Its implementation may provide an answer to the increasing pressure to optimize spending in healthcare while improving patient outcomes. This paper describes a pragmatic value-based healthcare framework for HIV care.

Methods
A value-based HIV healthcare framework was developed during a series of roundtable discussions bringing together 16 clinical stakeholder representatives from the Belgian HIV reference centers and 2 VBHC specialists. Each round of discussions was focused on a central question translating a concept or idea to the next level of practical implementation: 1) how can VBHC principles be translated into value-based HIV care drivers; 2) how can these value-based HIV care divers be translated into value-based care objectives and activities; and 3) how can value-based HIV care objectives and activities be translated into value-based care indicators. Value drivers were linked to concrete objectives and activities using a logical framework approach. Finally, specific, measurable, and acceptable structure, process and outcomes indicators were defined to complement the framework.

Results
Our framework identifies 4 core value areas where HIV care would benefit most from improvements: Prevention, improvement of the cascade of care, providing patient-centered HIV care and sustaining a state-of-the-art HIV disease management context. These 4 core value areas were translated into 12 actionable core value objectives. For each objective, example activities were proposed. Indicators are suggested for each level of the framework (outcome indicators for value areas and objectives, process indicators for suggested activities).

Conclusions
This framework approach outlines how to define a patient- and public health centered value-based HIV care paradigm. It proposes how to translate core value drivers to practical objectives and activities and suggests defining indicators that can be used to track and improve the framework’s implementation in practice.
A qualitative analysis of the barriers and enablers faced by Australian rural general practitioners in the non-pharmacological management of congestive heart failure in community dwelling patients
Authors: Kwan Trevor , Chua Benjamin , Pires David , Feng Olivia , Edmiston Natalie , Longman Jo 
Published: January 2022

Abstract
Background
Congestive heart failure (CHF) is a significant health problem in Australia, and disproportionately affects rural Australians. Management of CHF in Australia is heavily centred around the general practitioner (GP). Australian and international literature indicates there is a gap between current and best practice in CHF management. There is little known about the non-pharmacological aspects of management, or CHF management in a rural Australian context. This study aimed to identify what Australian GPs practicing in the Northern Rivers Region of New South Wales, Australia, perceived were the barriers and enablers in the non-pharmacological management of CHF amongst community dwelling patients, to inform healthcare access, resourcing and delivery in Australian rural environments.

Methods
Qualitative study involving a realist thematic analysis of data collected from semi-structured face-to-face interviews.

Results
Fifteen GPs and GP trainees participated. Four interlinked key themes underpinning GPs’ experiences with non-pharmacological management of CHF were interpreted from the interview data: (1) resources, (2) complexity of heart failure, (3) relationships, and (4) patient demographics, priorities and views affect how patients engage with non-pharmacological management of CHF.

Conclusion
Rural Australian GPs face considerable barriers to non-pharmacological management of CHF. The data suggests that increased rural Australian health services and community transportation, multidisciplinary management, and stronger professional networks have the potential to be invaluable enablers of CHF management. Further research exploring non-pharmacological management of CHF in other rural contexts may provide additional insights to better inform rural healthcare access and resourcing.
The roles, activities and impacts of middle managers who function as knowledge brokers to improve care delivery and outcomes in healthcare organizations: a critical interpretive synthesis
Authors: Boutcher Faith , Berta Whitney , Urquhart Robin , Gagliardi Anna R. 
Published: January 2022

Abstract
Background
Middle Managers (MMs) are thought to play a pivotal role as knowledge brokers (KBs) in healthcare organizations. However, the role of MMs who function as KBs (MM KBs) in health care is under-studied. Research is needed that contributes to our understanding of how MMs broker knowledge in health care and what factors influence their KB efforts.

Methods
We used a critical interpretive synthesis (CIS) approach to review both qualitative and quantitative studies to develop an organizing framework of how MMs enact the KB role in health care. We used compass questions to create a search strategy and electronic searches were conducted in MEDLINE, CINAHL, Social Sciences Abstracts, ABI/INFORM, EMBASE, PubMed, PsycINFO, ERIC and the Cochrane Library. Searching, sampling, and data analysis was an iterative process, using constant comparison, to synthesize the results.

Results
We included 41 articles (38 empirical studies and 3 conceptual papers) that met the eligibility criteria. No existing review was found on this topic. A synthesis of the studies revealed 12 MM KB roles and 63 associated activities beyond existing roles hypothesized by extant theory, and we elaborate on two MM KB roles: 1) convincing others of the need for, and benefit of an innovation or evidence-based practice; and 2) functioning as a strategic influencer. We identified organizational and individual factors that may influence the efforts of MM KBs in healthcare organizations. Additionally, we found that the MM KB role was associated with enhanced provider knowledge, and skills, as well as improved organizational outcomes.

Conclusion
Our findings suggest that MMs do enact KB roles in healthcare settings to implement innovations and practice change. Our organizing framework offers a novel conceptualization of MM KBs that advances understanding of the emerging KB role that MMs play in healthcare organizations. In addition to roles, this study contributes to the extant literature by revealing factors that may influence the efforts and impacts of MM KBs in healthcare organizations. Future studies are required to refine and strengthen this framework.

Trial registration
A protocol for this review was not registered.