bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribution of anxiety and depression among different subtypes of temporomandibular disorder: A systematic review and meta‐analysis
Authors: Reis Pedro Henrique Feres , Laxe Laisa Araújo Cortines , Lacerda‐Santos Rogério , Münchow Eliseu Aldrighi 
Published: April 2022

AbstractBackgroundThe evidence on the relationship between anxiety and depression and patients with distinct subtypes of temporomandibular disorder (TMD) is uncertain, so a thorough review study on the topic is still missing.ObjectivesThis systematic review investigated the distribution and severity of anxiety and depression in patients diagnosed with different subtypes of TMD.MethodsThe study is registered in PROSPERO (CRD42020150562) and it followed the PRISMA 2020 Statement. We searched in PubMed, Web of Science, Scopus and SciELO databases (last search: 12 March 2021) and the reference list from the included studies. Study eligibility criteria consisted of: (i) patients diagnosed with TMD using the Research Diagnostic Criteria (RDC/TMD) or Diagnostic Criteria (DC/TMD) instruments; (ii) assessment of anxiety and/or depression with validated psychological instruments and (iii) allocation of patients into a minimum of two distinct TMD subtypes with at least one group having myofascial pain (comparison group). Analyses were carried out using RevMan 5.3.5 statistical package and random‐ or fixed‐effects models (α = 0.05). The quality of evidence was assessed based on review authors' judgment derived from a 10‐item appraisal tool for prevalence studies and with the Newcastle–Ottawa scale.ResultsOf the 4086 records identified in total, 24 were eligible for inclusion; meta‐analyses were conducted with 20 studies. In total, 3678 subjects were included in the review. Most of the studies found that patients with myofascial pain showed similar occurrence and severity of anxiety/depression as compared to other subtypes of TMD, although the average prevalence seemed to be higher among the diagnoses consisting of myofascial pain (muscular TMD). Despite the moderate‐to‐high heterogeneity, anxiety and depression were more frequently distributed within patients with myofascial pain (p = .001). TMD patients without myofascial pain presented less severe levels of anxiety and depression than patients with only myofascial pain (p ≤ .01). The type of psychological instrument seems to affect the assessment of both anxiety and depression emotional states.ConclusionThe findings of this review suggest that patients with myofascial pain are more anxious and more depressed than patients with other subtypes of TMD.ImplicationsConsidering that anxiety and depression are differently distributed within the TMD population, a proper assessment of the psychological state of patients seems essential to offer an adequate treatment and management of each specific subtype of TMD.
Differential temperature effects on photoperiodism in female voles: A possible explanation for declines in vole populations
Authors: van Rosmalen Laura , Riedstra Bernd , Beemster Nico , Dijkstra Cor , Hut Roelof A. 
Published: April 2022

AbstractMany mammalian species use photoperiod as a predictive cue to time seasonal reproduction. In addition, metabolic effects on the reproductive axis may also influence seasonal timing, especially in female small, short‐lived mammals. To get a better understanding of how annual cycling environmental cues impact reproductive function and plasticity in small, short‐lived herbivores with different geographic origins, we investigated the mechanisms underlying integration of temperature in the photoperiodic‐axis regulating female reproduction in a Northern vole species (tundra vole, Microtus oeconomus) and in a Southern vole species (common vole, Microtus arvalis). We show that photoperiod and temperature interact to determine appropriate physiological responses; there is species‐dependent annual variation in the sensitivity to temperature for reproductive organ development. In common voles, temperature can overrule photoperiodical spring‐programmed responses, with reproductive organ mass being higher at 10°C than at 21°C, whereas in autumn they are less sensitive to temperature. These findings are in line with our census data, showing an earlier onset of spring reproduction in cold springs, while reproductive offset in autumn is synchronized to photoperiod. The reproductive organs of tundra voles were relatively insensitive to temperature, whereas hypothalamic gene expression was generally upregulated at 10°C. Thus, both vole species use photoperiod, whereas only common voles use temperature as a cue to control spring reproduction, which indicates species‐specific reproductive strategies. Due to global warming, spring reproduction in common voles will be delayed, perhaps resulting in shorter breeding seasons and thus declining populations, as observed throughout Europe.
Population genomic evidence of selection on structural variants in a natural hybrid zone
Authors: Zhang Linyi , Chaturvedi Samridhi , Nice Chris C. , Lucas Lauren K. , Gompert Zachariah 
Published: April 2022

AbstractStructural variants (SVs) can promote speciation by directly causing reproductive isolation or by suppressing recombination across large genomic regions. Whereas examples of each mechanism have been documented, systematic tests of the role of SVs in speciation are lacking. Here, we take advantage of long‐read (Oxford nanopore) whole‐genome sequencing and a hybrid zone between twoLycaeidesbutterfly taxa (L.melissaand Jackson HoleLycaeides) to comprehensively evaluate genome‐wide patterns of introgression for SVs and relate these patterns to hypotheses about speciation. We found >100,000 SVs segregating within or between the two hybridizing species. SVs and SNPs exhibited similar levels of genetic differentiation between species, with the exception of inversions, which were more differentiated. We detected credible variation in patterns of introgression among SV loci in the hybrid zone, with 562 of 1419 ancestry‐informative SVs exhibiting genomic clines that deviated from null expectations based on genome‐average ancestry. Overall, hybrids exhibited a directional shift towards Jackson HoleLycaeidesancestry at SV loci, consistent with the hypothesis that these loci experienced more selection on average than SNP loci. Surprisingly, we found that deletions, rather than inversions, showed the highest skew towards excess ancestry from Jackson HoleLycaeides. Excess Jackson HoleLycaeidesancestry in hybrids was also especially pronounced for Z‐linked SVs and inversions containing many genes. In conclusion, our results show that SVs are ubiquitous and suggest that SVs in general, but especially deletions, might disproportionately affect hybrid fitness and thus contribute to reproductive isolation.
Lichen holobionts show compositional structure along elevation
Authors: Rolshausen Gregor , Dal Grande Francesco , Otte Jürgen , Schmitt Imke 
Published: April 2022

AbstractHolobionts are dynamic ecosystems that may respond to abiotic drivers with compositional changes. Uncovering elevational diversity patterns within these microecosystems can further our understanding of community‐environment interactions. Here, we assess how the major components of lichen holobionts—fungal hosts, green algal symbionts, and the bacterial community—collectively respond to an elevational gradient. We analyse populations of two lichen symbioses, Umbilicaria pustulata and U. hispanica, along an elevational gradient spanning 2100 altitudinal metres and covering three major biomes. Our study shows (i) discontinuous genomic variation in fungal hosts with one abrupt genomic differentiation within each of the two host species, (ii) altitudinally structured bacterial communities with pronounced turnover within and between hosts, and (iii) altitude‐specific presence of algal symbionts. Alpha diversity of bacterial communities decreased with increasing elevation. A marked turnover in holobiont diversity occurred across two altitudinal belts: at 11°C–13°C average annual temperature (here: 800–1200 m a.s.l.), and at 7°C–9°C average annual temperature (here: 1500–1800 m a.s.l.). The two observed zones mark a clustering of distribution limits and community shifts. The three ensuing altitudinal classes, that is, the most frequent combinations of species in holobionts, approximately correspond to the Mediterranean, cool‐temperate, and alpine climate zones. We conclude that multitrophic microecosystems, such as lichen holobionts, respond with concerted compositional changes to climatic factors that also structure communities of macroorganisms, for example, vascular plants.
Eumycetoma causative agents are inhibited in vitro by luliconazole, lanoconazole and ravuconazole
Authors: Nyuykonge Bertrand , Lim Wilson , van Amelsvoort Lukas , Bonifaz Alexandro , Fahal Ahmed , Badali Hamid , Abastabar Mahdi , Verbon Annelies , van de Sande Wendy 
Published: April 2022

AbstractIntroductionEumycetoma is a subcutaneous mutilating disease that can be caused by many different fungi. Current treatment consists of prolonged itraconazole administration in combination with surgery. In many centres, due to their slow growth rate, the treatment for eumycetoma is often started before the causative agent is identified. This harbours the risk that the causative fungus is not susceptible to the given empirical therapy. In the open‐source drug program MycetOS, ravuconazole and luliconazole were promising antifungal agents that were able to inhibit the growth of Madurella mycetomatis, the most common causative agent of mycetoma. However, it is currently not known whether these drugs inhibit the growth of other eumycetoma causative agents.Materials and methodsHere, we determined the in vitro activity of luliconazole, lanoconazole and ravuconazole against commonly encountered eumycetoma causative agents. MICs were determined for lanoconazole, luliconazole and ravuconazole against 37 fungal isolates which included Madurella species, Falciformispora senegalensis, Medicopsis romeroi and Trematosphaeria grisea and compared to those of itraconazole.ResultsRavuconazole, luliconazole and lanoconazole showed high activity against all eumycetoma causative agents tested with median minimal inhibitory concentrations (MICs) ranging from 0.008–2 µg/ml, 0.001–0.064 µg/ml and 0.001–0.064 µg/ml, respectively. Even Ma. fahalii and Me. romeroi, which are not inhibited in growth by itraconazole at a concentration of 4 µg/ml, were inhibited by these azoles.ConclusionThe commonly encountered eumycetoma causative agents are inhibited by lanoconazole, luliconazole and ravuconazole. These drugs are promising candidates for further evaluation as potential treatment for eumycetoma.
Exploring primary school years interactions around child weight: A qualitative meta‐synthesis of school staff, parent, and child views and experiences
Authors: Chisholm Anna , Coupe Nia , Ujhelyi Gomez Katalin , Hart Jo , Peters Sarah 
Published: April 2022

SummaryInteractions about children's weight and weight‐related behaviors occur from an early stage in school settings between various stakeholders and are often intended to facilitate weight‐related behavioral change in children and/or families. This meta‐synthesis (PROSPERO ‐ CRD42019133231) aimed to investigate stakeholder reported experiences and challenges of these encounters. Studies were eligible if they included school stakeholders (teaching or nonteaching staff, parents, caregivers, or children), explored communication topics related to child obesity (weight, diet or activity), were conducted within an early school setting (primary school stage or international equivalent), and used qualitative methods. Database searches conducted March–July 2019 (updated November 2020) identified 40 studies (2324 participants) from seven countries. Included studies were assessed for quality using the Critical Appraisal Skills Programme. Using inductive thematic analysis, we identified four core themes across this database: (1) “conversation characteristics and consequences,” (2) “missing components,” (3) “avoiding stigma,” and (4) “school responsibilities.” Overall, stakeholders recognized that schools are well‐positioned to provide positive influential messages about childhood obesity and reported that discussions on this topic do occur in early school settings but that stakeholders find them difficult, complex, and lack the necessary skills to deliver the nonjudgmental, consistent, and tailored support that they desire.
In vitro virucidal activity of mouthwashes on SARS‐CoV‐2
Authors: Buonavoglia Alessio , Lanave Gianvito , Marchi Serena , Lorusso Pantaleo , Montomoli Emanuele , Martella Vito , Camero Michele , Prati Carlo , Trombetta Claudia Maria 
Published: April 2022

AbstractObjectivesThe objective of the study was to evaluate the in vitro virucidal activity of commercial mouthwashes against SARS‐CoV‐2 and variants of concern.Materials and MethodsAntiviral activity was assessed at different time intervals, based on common use of these products by titrating residual viral infectivity on Vero E6 cells.ResultsAll the mouthwashes were effective to reduce the infectious titers of SARS‐CoV‐2 and its tested variants. Mouthwashes Listerine® Cool Mint milder taste and Listerine® Cavity Protection milder taste reduced the infectious viral titer by up to 3.9 log10 after 30 s, while mouthwash Cetilsan® Sugar Free was able to reduce the viral titer by 2.2–2.9 log10 at all tested time intervals. Mouthwash Curasept® ADS DNA Intensive treatment was less effective to decrease viral infectivity (0.7–2.2 log10 TCID50/ml at all tested time intervals). Interestingly, the Gamma variant appeared more resistant to treatment in vitro with the different mouthwashes.ConclusionsIn this study, we were able to assess the ability of different mouthwashes to in vitro decrease the infectivity of SARS‐CoV‐2 and its variants, and we observed that Gamma variant of concern was more resistant to treatment with mouthwashes.
Image collection and annotation platforms to establish a multi‐source database of oral lesions
Authors: Rajendran Senthilmani , Lim Jian Han , Yogalingam Kohgulakuhan , Kallarakkal Thomas George , Zain Rosnah Binti , Jayasinghe Ruwan Duminda , Rimal Jyotsna , Kerr Alexander Ross , Amtha Rahmi , Patil Karthikeya , Welikala Roshan Alex , Lim Ying Zhi , Remagnino Paolo , Gibson John , Tilakaratne Wanninayake Mudiyanselage , Liew Chee Sun , Yang Yi‐Hsin , Barman Sarah Ann , Chan Chee Seng , Cheong Sok Ching 
Published: April 2022

AbstractObjectiveTo describe the development of a platform for image collection and annotation that resulted in a multi‐sourced international image dataset of oral lesions to facilitate the development of automated lesion classification algorithms.Materials and MethodsWe developed a web‐interface, hosted on a web server to collect oral lesions images from international partners. Further, we developed a customised annotation tool, also a web‐interface for systematic annotation of images to build a rich clinically labelled dataset. We evaluated the sensitivities comparing referral decisions through the annotation process with the clinical diagnosis of the lesions.ResultsThe image repository hosts 2474 images of oral lesions consisting of oral cancer, oral potentially malignant disorders and other oral lesions that were collected through MeMoSA® UPLOAD. Eight‐hundred images were annotated by seven oral medicine specialists on MeMoSA®ANNOTATE, to mark the lesion and to collect clinical labels. The sensitivity in referral decision for all lesions that required a referral for cancer management/surveillance was moderate to high depending on the type of lesion (64.3%–100%).ConclusionThis is the first description of a database with clinically labelled oral lesions. This database could accelerate the improvement of AI algorithms that can promote the early detection of high‐risk oral lesions.
Perceptions and experiences of functional mobility for community‐dwelling older people: A focused ethnography
Authors: Wisnesky Uirá Duarte , Paul Pauline , Olson Joanne , Dahlke Sherry 
Published: April 2022

AbstractBackgroundMobility challenges may indicate functional decline and are a relevant problem faced by older people. An individual’s perception of mobility is affected and moderated by several factors and varies significantly from one community to another. Providing an improved understanding of how older people perceive and experience mobility is a step towards better preparing nurses for roles they are increasingly expected to play.PurposeThe purpose of the study was to investigate perceptions and experiences of mobility in a group of Brazilian community‐dwelling older people living with mobility challenges.MethodData were generated between June and October 2018 through in‐depth interviews, socio‐demographic surveys and field notes. Purposive sampling strategies were used to recruit 23 Brazilian older people to participate in this focused ethnographic study. Data were analysed using qualitative content analysis.FindingsPerceptions of Brazilian community‐dwelling older people living with mobility challenges were culturally learned and influenced by the social, physical and health environments. Participants’ previous experiences with mobility had significant implications on values they attributed to mobility challenges. In addition, older people’s experiences with mobility challenges were an integral part of sense of self and strongly related to contextual factors.ConclusionsThis study contributes new information to the field of inquiry on mobility of older people as it focuses on the perceptions of a specific age group and culture and attends to the interrelationships between health conditions and contextual factors affecting the mobility of older people.Implications for practiceThis study’s particular contributions to the nursing discipline are related to improving care by revealing the significance of the context of mobility in which nursing actions are applied.
Compatibility of intravenous acetaminophen with morphine, fentanyl and ketamine in acute pediatric pain setting
Authors: O'Loughlin Edmond , Peng Yan Ghee , Cheaib Angela , Chan Matthew T. V. , Williams Robin 
Published: April 2022

AbstractBackgroundIntravenous acetaminophen and opioid analgesics are routinely given concurrently to children after major surgery, where intravenous access can be limited. There is limited information about the compatibility of acetaminophen with opioid analgesics and ketamine in concentrations commonly used in pediatric setting.AimsWe aimed to determine the physical and chemical compatibility in mixtures of intravenous acetaminophen 10 mg/mL with morphine (0.025, 0.05, 0.1, 0.2, 1, and 2 mg/ml), fentanyl (0.5, 0.75, 1, 1.5, 2, and 5 mcg/ml) and ketamine (0.01, 0.1, 0.5, 1, 1.5, and 3 mg/ml).MethodsAcetaminophen was mixed with all 18 solutions and was examined at time 0, 15, 30, and 60 min. In Phase one of the study, we used a colorimetric method to assess preliminary feasibility and acetaminophen recovery. In Phase two study, we used high‐performance liquid chromatography to evaluate the recovery of all components of the mixtures.ResultsAll solutions tested, for both acetaminophen and the three analgesics, had more than 90% of the drug recovery, up to 60 min after mixing.ConclusionOur data demonstrated the stability of acetaminophen, in combination with fentanyl, morphine, and ketamine at clinical concentrations used in acute pediatric pain setting.
PPVED: A machine learning tool for predicting the effect of single amino acid substitution on protein function in plants
Authors: Gou Xiangjian , Feng Xuanjun , Shi Haoran , Guo Tingting , Xie Rongqian , Liu Yaxi , Wang Qi , Li Hongxiang , Yang Banglie , Chen Lixue , Lu Yanli 
Published: April 2022

SummarySingle amino acid substitution (SAAS) produces the most common variant of protein function change under physiological conditions. As the number of SAAS events in plants has increased exponentially, an effective prediction tool is required to help identify and distinguish functional SAASs from the whole genome as either potentially causal traits or as variants. Here, we constructed a plant SAAS database that stores 12 865 SAASs in 6172 proteins and developed a tool called Plant Protein Variation Effect Detector (PPVED) that predicts the effect of SAASs on protein function in plants. PPVED achieved an 87% predictive accuracy when applied to plant SAASs, an accuracy that was much higher than those from six human database software: SIFT, PROVEAN, PANTHER‐PSEP, PhD‐SNP, PolyPhen‐2, and MutPred2. The predictive effect of six SAASs from three proteins in Arabidopsis and maize was validated with wet lab experiments, of which five substitution sites were accurately predicted. PPVED could facilitate the identification and characterization of genetic variants that explain observed phenotype variations in plants, contributing to solutions for challenges in functional genomics and systems biology. PPVED can be accessed under a CC‐BY (4.0) license via http://www.ppved.org.cn.
Effect of Photobiomodulation on Structure and Function of Extracellular Vesicle Secreted from Mesenchymal Stem Cells
Authors: Gholami Leila , Khorsandi Khatereh , Nooshabadi Vajihe Taghdiri , Shahabi Shiva , Jazaeri Marzieh , Esfahani HomaSadat , Faradonbeh Davood Rabiei , Malekshahi Ziba Veisi , Afsartala Zohreh , Mostafa Nesrine 
Published: April 2022

ABSTRACTThe current study intended to evaluate the effect of photobiomodulation on the morphology and function of EVs secreted from mesenchymal stem cells (MSCs) derived from periodontal ligament (PDL) and the adipose tissue (ADSCs) (from buccal fat pad) in vitro. These cells were irradiated at 660 nm or kept in dark as control. EVs were then isolated from each group using ultracentrifugation. EVs were defined by flow cytometry and Western blot. Electron microscopy (SEM) was used to study the morphology of EVs. Then, MTT and wound‐healing scratch assays were applied to compare the cell survival and migration of human dermal fibroblast (HDF) cells treated with the EVs obtained from the four groups. According to SEM images, isolated EV were round and cup‐shaped in all groups showing no destructive effects of laser irradiation on EV morphology. MTT test results revealed a statistically significant difference between the HDF cells treated with different EV groups from hPDLSCs‐Dark in comparison with control (0 μg/mL) (P < 0.05) and treated with exosome from hPDLSCs‐Irradiation cells compared with dark group (P < 0.05). However, scratch wound‐healing assay did not show a significant difference between various groups (P ˃ 0.05). Further studies with different irradiation protocols are recommended to find an optimal strategy.