bluuebunny/crossref_metadata_2025_split huggingface dataset:
KSHV infection of endothelial precursor cells with lymphatic characteristics as a novel model for translational Kaposi’s sarcoma studies
Authors: Tuohinto Krista , DiMaio Terri A. , Kiss Elina A. , Laakkonen Pirjo , Saharinen Pipsa , Karnezis Tara , Lagunoff Michael , Ojala Päivi M. 
Published: July 2022

AbstractKaposi’s sarcoma herpesvirus (KSHV) is the etiologic agent of Kaposi’s sarcoma (KS), a hyperplasia consisting of enlarged malformed vasculature and spindle-shaped cells, the main proliferative component of KS. While spindle cells express markers of lymphatic and blood endothelium, the origin of spindle cells is unknown. Endothelial precursor cells have been proposed as the source of spindle cells. We previously identified two types of circulating endothelial colony forming cells (ECFCs), ones that expressed markers of blood endothelium and ones that expressed markers of lymphatic endothelium. Here we examined both blood and lymphatic ECFCs infected with KSHV. Lymphatic ECFCs are significantly more susceptible to KSHV infection than the blood ECFCs and maintain the viral episomes during passage in culture while the blood ECFCs lose the viral episome. Only the KSHV-infected lymphatic ECFCs grew to small multicellular colonies in soft agar whereas the infected blood ECFCs and all uninfected ECFCs failed to proliferate. The lymphatic ECFCs express high levels of SOX18, which supported the maintenance of high copy number of KSHV genomes. When implanted subcutaneously into NSG mice, the KSHV-infected lymphatic ECFCs persisted in vivo and recapitulated the phenotype of KS tumor cells with high number of viral genome copies and spindling morphology. These spindle cell hallmarks were significantly reduced when mice were treated with SOX18 inhibitor, SM4. These data suggest that KSHV-infected lymphatic ECFCs can be utilized as a KSHV infection model for in vivo translational studies to test novel inhibitors representing potential treatment modalities for KS.Author summaryKaposi’s sarcoma herpesvirus (KSHV) is the etiologic agent of Kaposi’s sarcoma (KS). The main proliferative component of KS, spindle cells, express markers of lymphatic and blood endothelium. Endothelial precursor cells, which are circulating endothelial colony forming cells (ECFCs), have been proposed as the source of spindle cells. Here we examined both blood and lymphatic ECFCs infected with KSHV. Lymphatic ECFCs are readily infected by KSHV, maintain the viral episomes and show minimal transformation of the cells, which the infected blood ECFCs and all uninfected ECFCs failed to show. The lymphatic ECFCs express SOX18, which supported the maintenance of high copy numbers of KSHV genomes. The KSHV-infected lymphatic ECFCs persisted in vivo and recapitulated the phenotype of KS tumor cells such as high number of viral genome copies and spindling morphology. These KS tumor cell hallmarks were significantly reduced by SOX18 chemical inhibition using a small molecule SM4 treatment. These data suggest that KSHV-infected lymphatic ECFCs could be the progenitors of KS spindle cells and are a promising model for the translational studies to develop new therapies for KS.
Poor sensitivity of iPSC-derived neural progenitors and glutamatergic neurons to SARS-CoV-2
Authors: Zivaljic Marija , Hubert Mathieu , Grzelak Ludivine , Sansone Giulia , Maskos Uwe , Schwartz Olivier 
Published: July 2022

AbstractCOVID-19 is a respiratory disease affecting multiple organs including the central nervous system (CNS), with a characteristic loss of smell and taste. Although frequently reported, the neurological symptoms remain enigmatic. There is no consensus on the extent of CNS infection. Here, we derived human induced pluripotent stem cells (hiPSC) into neural progenitor cells (NPCs) and cortical excitatory neurons to study their permissiveness to SARS-CoV-2 infection. Flow cytometry and western blot analysis indicated that NPCs and neurons do not express detectable levels of the SARS-CoV-2 receptor ACE2. We thus generated cells expressing ACE2 by lentiviral transduction to analyze in a controlled manner the properties of SARS-CoV-2 infection relative to ACE2 expression. Sensitivity of parental and ACE2 expressing cells was assessed with GFP- or luciferase-carrying pseudoviruses and with authentic SARS-CoV-2 Wuhan, D614G, Alpha or Delta variants. SARS-CoV-2 replication was assessed by microscopy, RT-qPCR and infectivity assays. Pseudoviruses infected only cells overexpressing ACE2. Neurons and NPCs were unable to efficiently replicate SARS-CoV-2, whereas ACE2 overexpressing neurons were highly sensitive to productive infection. Altogether, our results indicate that primary NPCs and cortical neurons remain poorly permissive to SARS-CoV-2 across the variants’ spectrum, in the absence of ACE2 expression.
Brainstem fMRI signaling of surprise across different types of deviant stimuli
Authors: Mazancieux Audrey , Mauconduit Franck , Amadon Alexis , de Gee Jan Willem , Donner Tobias , Meyniel Florent 
Published: July 2022

SummaryThe detection of deviant stimuli is crucial to orient and adapt our behavior. Previous work showed that infrequent (hence deviant) stimuli elicit phasic activation of the brainstem locus coeruleus (LC), which releases noradrenaline and controls central arousal. However, it is unclear whether the detection of behaviorally-relevant deviant events selectively trigger LC responses, or also other neuromodulatory systems related to dopamine, acetylcholine, and serotonin. Here, we combined human fMRI recordings optimized for brainstem imaging with pupillometry (a peripheral marker of central arousal) to perform a mapping of deviant-related responses in subcortical structures. Participants had to detect deviant items in a “local-global” paradigm that distinguishes between deviance based on the stimulus probability and the sequence structure. fMRI responses to deviant stimuli were quite distributed, detected in the LC but also other subcortical nuclei and many cortical areas. Both types of deviance elicited responses in the pupil, LC and other neuromodulatory systems. Our results reveal that the detection of task-relevant deviant items recruits the same multiple subcortical systems across computationally different types of deviance.
Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells
Authors: Seita Yasunari , Cheng Keren , McCarrey John R. , Yadu Nomesh , Cheeseman Ian , Bagwell Alec , Ross Corinna N. , Santana-Toro Isamar , Yen Li-Hua , Vargas Sean , Navara Christopher S. , Hermann Brian P. , Sasaki Kotaro 
Published: July 2022

ABSTRACTReconstitution of germ cell fate from pluripotent stem cells provides an opportunity to understand the molecular underpinnings of germ cell development. Here, we established robust methods for pluripotent stem cells (iPSCs) culture in the common marmoset (Callithrix jacchus, cj), which stably propagate in an undifferentiated state. Notably, iPSCs cultured on a feeder layer in the presence of a WNT signaling inhibitor upregulated genes related to ubiquitin-dependent protein catabolic processes and enter a permissive state that enables differentiation into primordial germ cell-like cells (PGCLCs) bearing immunophenotypic and transcriptomic similarities to pre-migratory cjPGCsin vivo. Induction of cjPGCLCs is accompanied by transient upregulation of mesodermal genes culminating in the establishment of a primate specific germline transcriptional network. Moreover, cjPGCLCs can be expanded in monolayer while retaining the germline state. Upon co-culture with mouse testicular somatic cells, these cells acquire an early prospermatogonia-like phenotype. Our findings provide a framework for understanding and reconstituting marmoset germ cell development in vitro, thus providing a comparative tool and foundation for a preclinical modeling of human in vitro gametogenesis.
Robustness of the neuro-muscular octopaminergic system in the face of cold stress
Authors: Kaya-Zeeb Sinan , Delac Saskia , Wolf Lena , Marante Ana Luiza , Scherf-Clavel Oliver , Thamm Markus 
Published: July 2022

ABSTRACTIn recent decades, our planet has undergone dramatic environmental changes resulting in the loss of numerous species. This contrasts with species that can adapt quickly to rapidly changing ambient conditions, which require physiological plasticity and must occur rapidly. The Western honeybee (Apis mellifera) apparently meets this challenge with remarkable success, as this species is adapted to numerous climates, resulting in an almost worldwide distribution. Here, coordinated individual thermoregulatory activities ensure survival at the colony level and thus the transmission of genetic material. Recently, we showed that shivering thermogenesis, which is critical for honeybee thermoregulation, depends on octopamine signaling. In this study, we tested the hypothesis that the thoracic neuro-muscular octopaminergic system strives for a steady-state equilibrium under cold stress to maintain endogenous thermogenesis. We can show that this applies for both, octopamine provision by flight muscle innervating neurons and octopamine receptor expression in the flight muscles. Additionally, we discovered alternative splicing forAmOARβ2and our results suggest that at least one isoform is needed to survive cold stress conditions. We assume that the thoracic neuro-muscular octopaminergic system is finely tuned in order to contribute decisively to survival in a changing environment.
The evolutionary dynamics that retain long neutral genomic sequences in face of indel deletion bias: a model and its application to human introns
Authors: Loewenthal Gil , Wygoda Elya , Nagar Natan , Glick Lior , Mayrose Itay , Pupko Tal 
Published: July 2022

AbstractInsertions and deletions (indels) of short DNA segments are common evolutionary events. Numerous studies showed that deletions occur more often than insertions in both prokaryotes and eukaryotes. It raises the question why neutral sequences are not eradicated from the genome. We suggest that this is due to a phenomenon we termborder-induced selection. Accordingly, a neutral sequence is bordered between conserved regions. Deletions occurring near the borders occasionally protrude to the conserved region and are thereby subject to strong purifying selection. Thus, for short neutral sequences, an insertion bias is expected. Here, we develop a set of increasingly complex models of indel-dynamics that incorporate border-induced selection. Furthermore, we show that short conserved sequences within the neutrally evolving sequence help explain: (1) the presence of very long sequences; (2) the high variance of sequence lengths; (3) the possible emergence of multimodality in sequence length distributions. Finally, we fitted our models to the human intron length distribution, as introns are thought to be mostly neutral and bordered by conserved exons. We show that when accounting for the occurrence of short conserved sequences within introns, we reproduce the main features, including the presence of long introns and the multimodality of intron distribution.
Challenges for lean construction adoption in the Brazilian industry: a study in construction companies, universities and class organizations
Authors: Silva Edson Neves da , Brasil de Brito Mello Luiz Carlos , Pinto Gustavo Oliveira 
Published: July 2022

Purpose
This paper aims to evaluate the current level of knowledge about lean construction (LC) practices in construction companies, universities and class organizations in Brazil and identify the barriers to implementing LC in the Brazilian construction industry and prioritize them through comparison with global challenges.

Design/methodology/approach
This multiple case study was carried out through a questionnaire answered by 56 construction companies, 15 universities and 10 professional associations. The questionnaire evaluation allowed the authors to determine the level of knowledge about the LC, its adoption and barriers to its adoption in the Brazilian industry. The results were statistically analyzed using the weighted average (MIS) of results using the Kruskal–Wallis test, which compares sample distribution among groups, and Cronbach’s alpha test to measure the questionnaire reliability.

Findings
This study points to universities as the main ones responsible for the low adoption of LC in Brazil and points to the need for an integrated action among construction companies, universities and class organizations to overcome the traditional project management culture and raise the level of knowledge about LC.

Originality/value
This study evaluated the current LC situation in an emerging country, Brazil, and compared it with the principal international challenges observed in studies of other developing countries – China, India, Morocco, Saudi Arabia and Singapore – least developed countries – Bangladesh and Nigeria – and developed country – UK. These studies evaluated construction companies located in the listed countries. Based on that, 22 international challenges were established for the LC adoption. Additionally, this research also evaluated universities and working-class organizations in addition to construction companies. This addition can contribute to a better result understanding that can be explored by Brazil and other countries.
“Live within the messiness”: how a digitally mediated inquiry community supported ELA teachers in cultivating adaptive repertoires
Authors: Monea Bethany , Burrows-Stone Katie , Dunbar Jennifer Griffith , Freed Jennifer , Stornaiuolo Amy , Griffin Autumn A. 
Published: July 2022

PurposeAdaptivity has long been recognized as a key aspect of teaching and shown to be particularly important for English Language Arts (ELA) teachers leading discussions about texts. Teachers' abilities to make such adjustments are especially important when facilitating discussions in digital contexts, as was made clear with the shift to virtual teaching caused by the COVID-19 pandemic. The purpose of this paper is to explore how the structure and process of teacher inquiry supported ELA teachers in enacting and cultivating adaptive repertoires for facilitating discussion in online contexts during the disruptions of the 2020–2021 school year.Design/methodology/approachAs an inquiry team comprising teacher-researchers from secondary and university-based contexts, the authors used practitioner inquiry methods in the context of a multi-year, multi-sited study involving, design-based and teacher-research methodologies.FindingsThis paper shows how teachers’ engagement in digital teacher inquiry groups supported their willingness to be playful and adapt their practices in response to one another, creating conditions for powerful teacher learning through relational inquiry online. This paper identified three specific relational practices that were critical for cultivating adaptive repertories in teachers’ learning with and from each other: cultivating empathy; attuning to silences and actively listening; and decentering authority across multiple platforms and modalities. The authors discuss how teachers carried these practices to and from their digital discussions with their students and with each other, demonstrating how this recursive cycle of inquiry and practice deepened their learning, relationships and adaptive repertoires.Originality/valueThe authors discuss the implications of these practices for equity-oriented and dialogic teacher learning that can transform classroom practice, illustrating the power of online teacher inquiry groups for developing ELA teachers’ adaptive expertise – something urgently important for teaching in digitally mediated contexts and through unsettled times.
How employees leverage psychological capital and perform, even in the presence of rude co-workers: an empirical study from Pakistan
Authors: Azeem Muhammad Umer , De Clercq Dirk , Haq Inam Ul 
Published: July 2022

Purpose
This study aims to unpack the link between co-worker incivility and job performance, by detailing a mediating role of psychological detachment and a moderating role of psychological capital.

Design/methodology/approach
The research hypotheses are tested with three-wave, time-lagged data collected from Pakistani-based employees and their supervisors.

Findings
An important reason that disrespectful co-worker treatment curtails job performance, with respect to both in-role and extra-role work efforts, is that employees detach from their work environment. This mediating role of psychological detachment is less salient to the extent that employees possess high levels of psychological capital.

Practical implications
For organizations, this study pinpoints a key mechanism, a propensity to distance oneself from work, by which convictions that co-workers do not show respect direct employees away from productive work activities. This study also shows how this mechanism can be subdued by ensuring that employees exhibit energy-enhancing personal resources.

Originality/value
This study expands extant research on the dark side of interpersonal co-worker relationships by revealing pertinent factors that explain why and when co-worker incivility can escalate into diminished performance-enhancing activities.
Internal factors affecting the performance of employee-based savings and credit cooperatives: evidence from Nigeria
Authors: Otache Innocent , Echukwu Ifeoma Jeraldine , Umar Kadiri , Yunusa Acho , Audu Samson 
Published: July 2022

Purpose
Drawing upon stewardship and resource-based view theories, the purpose of this study is to empirically examine the impacts of management committee effectiveness (MCE), member economic participation (MEP), innovation (INNOV) and internal control systems (ICS) on the performance of employee-based savings and credit cooperatives (SACCOs) in Nigeria.

Design/methodology/approach
This study adopted a survey research design. Thus, a structured questionnaire was used to collect data from a sample of 295 members of six employee-based SACCOs in Nigeria. To test the study hypotheses, partial least squares structural equation modelling (PLS-SEM), through SmartPLS version 2, was used.

Findings
The results show that MCE, MEP, INNOV and ICS have significant positive links with the performance of employee-based SACCOs. Further analysis reveals that MCE has the greatest impact on performance, followed by MEP, ICS and INNOV, respectively.

Practical implications
The findings provide practical and managerial implications for members and management committees of employee-based SACCOs.

Originality/value
There is a paucity of studies on the impacts of MCE, MEP, INNOV and ICS on cooperative performance. This study contributes to the literature on cooperatives by demonstrating the positive impacts of MCE, MEP, INNOV and ICS on cooperative performance in a single study.
Beauty lies in the eye of the beholder: consumers' and jobseekers' interpretations of the family business brand
Authors: Jaufenthaler Philipp 
Published: July 2022

Purpose
Brands are relevant to multiple stakeholders and, as such, can have multiple meanings. Drawing on branding and stakeholder marketing theory, the purpose of this study was to provide in-depth insights into knowledge dynamics related to the so-called family business brand across different stakeholder perspectives.

Design/methodology/approach
This study combines advanced brand concept mapping (BCM) with a scenario technique to graphically capture consumers’ and jobseekers’ brand knowledge related to the family business concept in the form of association networks.

Findings
Findings show both a shared core meaning (e.g. associations such as “tradition” or “owner-managed”) and stakeholder-specific associations (e.g. consumer perspective: “high product quality,” “handmade”; jobseeker perspective: “appreciation within the company,” “outdated”). Significantly, the results reveal detailed insights into cross-stakeholder knowledge dynamics regarding the associations’ interconnections, strength and favorability.

Originality/value
By combining BCM with a scenario technique, this study adopts an approach that is aimed at better understanding and comparing brand knowledge with respect to the family business brand across multiple stakeholder perspectives. Given the prevalence of family businesses, this research enhances the theoretical and practical understanding of a branding resource that is often available but rarely exploited.
Living with tensions in the workplace: a grounded theory of paradoxical leadership in cultivating subordinates' paradox mindset
Authors: Yin Jun 
Published: July 2022

PurposeThe purpose of this paper is to investigate the process of how paradoxical leadership cultivates subordinates' paradox mindset.Design/methodology/approachThis study used the grounded theory approach. Data collection included semi-structured interviews and observations from eleven top managers and their six subordinates in five Chinese organizations. Managers and their subordinates were asked how they perceived and dealt with tensions in the workplace.FindingsParadoxical leadership promotes subordinates' paradox mindset by helping them learn to face, cope, and live with tensions. First, paradoxical leaders acted as role models for their subordinates and directly influenced followers' attitudes, motivation, and positive psychological states towards tensions. Moreover, when managers help their subordinates reduce stress and anxiety and build confidence, followers learn to cope with tensions. Consequently, subordinates' capacity to live with tensions is increased and they develop the paradox mindset.Practical implicationsThe results imply that reducing subordinates' stress and anxiety and building their confidence can be integrated into work practices and training, which provide insights for managers to cultivate their subordinates' paradox mindset.Originality/valueThis paper investigates how paradoxical leadership impacts and cultivates subordinates' paradox mindset in a tension-setting work environment. The findings not only provide fresh insights into the detailed process of developing employees' paradox mindset but also deepen the understanding of the leader–follower relationship.