bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the social dynamics of urban regeneration: A qualitative analysis of community members' experiences
Authors: Heath Stacey C. , Rabinovich Anna , Barreto Manuela 
Published: September 2022

AbstractThe present paper explores psychological processes that underpin the success of community change in the context of urban regeneration schemes. We adopt a social identity approach to develop an understanding of the ways in which social identity dynamics may impact upon peoples' experiences of regeneration, and what influence these identity processes have on the creation of new communities. Qualitative interviews, using thematic analysis as an analytic technique, were conducted with community members (n = 14) in a recently (2001–2011) regenerated area in the South‐West of England. Three overarching themes were identified: Patterns of identification, willingness to engage, and the notion of regeneration as an event. The research overall highlights the central role of group‐based identity in understanding the processes of regeneration and how this is experienced by different community members. Findings are discussed in relation to the impact regeneration schemes have on community members' sense of collective self, unity, and engagement. The research highlights the pivotal role of social identity processes in delivering successful and sustainable strategies of urban regeneration.
Marked oestrous cycle‐dependent regulation of rat arterial KV7.4 channels driven by GPER1
Authors: Baldwin Samuel N. , Forrester Elizabeth A. , Homer Natalie Z. M. , Andrew Ruth , Barrese Vincenzo , Stott Jennifer B. , Isakson Brant E. , Albert Anthony P. , Greenwood Iain A. 
Published: September 2022

Background and PurposeKcnq‐encoded KV7 channels (termed KV7.1–5) regulate vascular smooth muscle cell (VSMC) contractility at rest and as targets of receptor‐mediated responses. However, the current data are mostly derived from males. Considering the known effects of sex, the oestrous cycle and sex hormones on vascular reactivity, here we have characterised the molecular and functional properties of KV7 channels from renal and mesenteric arteries from female Wistar rats separated into di‐oestrus and met‐oestrus (F‐D/M) and pro‐oestrus and oestrus (F‐P/E).Experimental ApproachRT‐qPCR, immunocytochemistry, proximity ligation assay and wire myography were performed in renal and mesenteric arteries. Circulating sex hormone concentrations were determined by liquid chromatography–tandem mass spectrometry. Whole‐cell electrophysiology was undertaken on cells expressing KV7.4 channels in association with G‐protein‐coupled oestrogen receptor 1 (GPER1).Key ResultsThe KV7.2–5 activators S‐1 and ML213 and the pan‐KV7 inhibitor linopirdine were more effective in arteries from F‐D/M compared with F‐P/E animals. In VSMCs isolated from F‐P/E rats, exploratory evidence indicates reduced membrane abundance of KV7.4 but not KV7.1, KV7.5 and Kcne4 when compared with cells from F‐D/M. Plasma oestradiol was higher in F‐P/E compared with F‐D/M, and progesterone showed the converse pattern. Oestradiol/GPER1 agonist G‐1 diminished KV7.4 encoded currents and ML213 relaxations and reduced the membrane abundance of KV7.4 and interaction between KV7.4 and heat shock protein 90 (HSP90), in arteries from F‐D/M but not F‐P/E.Conclusions and ImplicationsGPER1 signalling decreased KV7.4 membrane abundance in conjunction with diminished interaction with HSP90, giving rise to a ‘pro‐contractile state’.
Multiomics analyses and machine learning of nuclear receptor coactivator 6 reveal its essential role in hepatocellular carcinoma
Authors: Fang Yinghao , Xu Yuyan , Liao Wei , Ji Tao , Yu Linyuan , Li Longhai , Pan Mingxin , Yang Dinghua 
Published: September 2022

AbstractNuclear receptor coactivator 6 (NCOA6), a coactivator of numerous nuclear receptors and transcription factors, regulates multiple critical cellular functions. Nuclear receptor coactivator 6 is dysregulated in various cancers, including hepatocellular carcinoma (HCC); however, its role remains largely unknown. Here we reported that NCOA6 was highly expressed in HCC compared to the adjacent liver tissue, and NCOA6 overexpression was significantly correlated with poor HCC prognosis. Experiments revealed that the knockdown of NCOA6 damaged the proliferation, migration, and invasion of HCC cells. Multiomics and immune infiltration analyses showed a close relationship between NCOA6 expression, multiple cancer‐related malignant pathways, and the immunosuppressive microenvironment. Finally, we established an effective NCOA6‐related microRNA (miRNA) signature to distinguish HCC from hepatitis\liver cirrhosis patients. To the best of our knowledge, this study is the first to provide a comprehensive analysis of NCOA6 expression in HCC. We found that NCOA6 plays an important role in HCC development and has a potential mechanism of action. Establishing an NCOA6‐related miRNA signature will help develop novel diagnostic strategies for HCC patients.
N6‐methyladenosine‐modified HOTAIRM1 promotes vasculogenic mimicry formation in glioma
Authors: Wu Zhangyi , Lin Yihai , Wei Nan 
Published: September 2022

AbstractVasculogenic mimicry (VM) has been reported to accelerate angiogenesis in malignant tumors, yet the mechanism underlying VM has not been fully elucidated. N6‐methyladenosine (m6A) mainly modulates mRNA fate and affects multiple tumorigenesis. Here, we aimed to investigate m6A‐modified HOXA transcript antisense RNA myeloid‐specific 1 (HOTAIRM1) in the regulation of glioma‐associated VM formation. Gene expression was analyzed by quantitative RT‐PCR. Cell viability, metastases, and VM formation capacity were determined by CCK‐8, migration and invasion, as well as tube formation assays, respectively. The function and mechanisms of m6A‐modified HOTAIRM1 were defined through liquid chromatography–tandem mass spectrometry m6A quantification, methylated RNA immunoprecipitation sequencing, RNA stability assays, and RNA pull‐down experiments. A glioma xenograft mouse model was further established for VM evaluation in vivo. The results showed that HOTAIRM1, methyltransferase‐like 3 (METTL3), and insulin‐like growth factor binding protein 2 (IGFBP2) were upregulated in glioma tissues and cell lines. HOTAIRM1 functions as an oncogene in glioma progression; however, knockdown of HOTAIRM1 significantly reduced cell viability, migration, invasion, and VM formation. Notably, METTL3‐dependent m6A modification enhanced HOTAIRM1 mRNA stability, whereas knockdown of METTL3 deficiency significantly suppressed VM in glioma. Moreover, HOTAIRM1 was found to bind IGFBP2, and HOTAIRM1 deficiency blocked glioma progression and VM formation in vivo. Our results indicated that METTL3‐dependent m6A‐modified HOTAIRM1 promoted VM formation in glioma.
The expression of miR‐181b, CYLD, CBX‐7, BCL2, and p53 in osteosarcoma patients and correlation with clinicopathological factors
Authors: Fazli Jafar , Fattah Khashayar , Moliani Afshin , Valizadeh Amir , Bazavar Mohammadreza 
Published: September 2022

AbstractOsteosarcoma is a common human malignancy with a high mortality rate worldwide. Recent studies have been focused on understanding the involvement of microRNA (miRNAs) in the pathogenesis of osteosarcoma. Therefore, the present study aimed to measure the expression levels of miR‐181a, cylindromatosis (CYLD), chromo box homolog 7 (CBX7), B‐cell lymphoma 2 (BCL2), and tumor protein p53 in tumor tissue and adjacent normal tissues in patients with osteosarcoma and its relationship with clinicopathological factors. The expression levels of miR‐181a, CYLD, CBX7, BCL2, and p53 were measured in 60 patients with osteosarcoma using quantitative real‐time polymerase chain reaction. Finally, we compared the relationship between these gene levels and clinicopathological factors in tumor and healthy tissues. Our results showed that the expression levels of miR‐181a, BCL2, and p53 were significantly higher in osteosarcoma tissue in comparison with normal tissues (p < .05). On the contrary, CYLD and CBX7 were downregulated in osteosarcoma tumor tissues compared to adjacent healthy tissues (p < .05). In addition, the expression levels of miR‐181a in tumor tissues were strongly correlated with patients' age, tumor size, clinical stage, cancer grade, and lymph node metastasis (p < .05). Our findings highlight new insights into understanding the role of miR‐181a in the pathogenesis of osteosarcoma. However, further studies are needed to elucidate miRNA as therapeutic targets for osteosarcoma.
An in vitro assessment of the thermoreversible PLGA‐PEG‐PLGA copolymer: Implications for Descemet's membrane endothelial keratoplasty
Authors: Tint Naing L. , Cheng Kelvin K. W. , Dhillon Amritpaul S. , Keane Pearse A. , Alexander Philip , Kennedy David , Chau David Y. S. , Rose Felicity R. A. J. , Allan Bruce D. S. 
Published: September 2022

AbstractBackgroundTo explore the use of a thermoreversible copolymer gel coating to prevent donor tissue scrolling in Descemet's membrane endothelial keratoplasty (DMEK).MethodsPLGA‐PEG‐PLGA triblock copolymer was synthesised via ring opening polymerisation. Two formulations were fabricated and gelation properties characterised using rheological analyses. Endothelial cytotoxicity of the copolymer was assessed using a Trypan Blue exclusion assay. Thickness of the copolymer gel coating on the endothelial surface was analysed using anterior segment optical coherence tomography (OCT) (RTVue‐100, Optovue Inc.). Gold nanoparticles were added to the copolymer to aid visualisation using OCT. Prevention of Descemet membrane donor scrolling was represented via a novel, in vitro, immersion of copolymer coated donor graft material.ResultsTwo different formulations of PLGA‐PEG‐PLGA copolymer were successfully fabricated and the desired peak gelling temperature of 24°C was achieved by polymer blending. Application of 20%, 30% and 40% (wt/vol) polymer concentrations resulted in a statistically significant increase in polymer thickness on the endothelium (p < 0.001). There was no detectable endothelial cytotoxicity. The polymer was easy to apply to the endothelium and prevented scrolling of the DMEK graft.ConclusionThis PLGA‐PEG‐PLGA thermoreversible copolymer gel could be exploited as a therapeutic aid for preventing DMEK graft scrolling.
Characteristics and prognosis analysis of bilateral sudden sensorineural hearing loss: A retrospective case–control study
Authors: Wang Yingjun , Xiong Wenping , Sun Xiao , Liu Wenwen , Fan Zhaomin , Wang Haibo , Wang Mingming 
Published: September 2022

AbstractObjectivesTo evaluate the clinical characteristics and prognostic factors of simultaneous and sequential bilateral sudden sensorineural hearing loss (Si‐BSSNHL and Se‐BSSNHL, respectively).DesignRetrospective case–control study.SettingA single tertiary referral centre.ParticipantsPatients diagnosed with unilateral sudden sensorineural hearing loss (USSNHL), Si‐BSSNHL, or Se‐BSSNHL between September 2018 and November 2019.Main outcome measuresDemographic and clinical characteristics, audiological features, laboratory results and hearing recovery were analysed for intergroup comparisons. Prognostic factors for BSSNHL were analysed using univariate and multivariate logistic analyses between the overall and no‐recovery groups.ResultsCompared to the USSNHL group, a larger final pure‐tone average (PTA) (H = 38.0 and 53.8, respectively, both adjusted p‐value (p adj) <.05), lower hearing gain (H = −70.8 and − 74.6, respectively, both p adj <.001) and higher homocysteine levels (H = 46.8, 58.8, respectively, both p adj <.05) were observed in the Si‐BSSNHL and Se‐BSSNHL groups, while the rate of positive vestibular tests and proportion of tinnitus were lower in the Se‐BSSNHL group (χ2 = 8.5 and 38.1, respectively, both p adj <0.05). The USSNHL group showed a significant difference in the degree of deafness and therapeutic outcome in the Se‐BSSNHL and Si‐BSSNHL groups, respectively (χ2 = 12.4, p adj <.05; χ2 = 13.6; p adj <.05). Hypertension (95% confidence interval, 1.014–28.623, p < .05) and onset days (95% confidence interval, 0.007–0.626, p < .05) were associated with the therapeutic effects of BSSNHL.ConclusionsHigher homocysteine levels in BSSNHL may implicate microvascular disorders as a causative factor of BSSNHL. Hypertension and onset days were associated with the prognosis of BSSNHL.
Quantitative tools and measurements for assessing the implementation of regulatory policies in reducing alcohol consumption and alcohol‐related harms: A scoping review
Authors: Jankhotkaew Jintana , Casswell Sally , Huckle Taisia , Chaiyasong Surasak , Phonsuk Payao 
Published: September 2022

AbstractIssuesImplementation of alcohol control policy is a global priority as alcohol contributes to negative individual health and societal impacts. However, there are no available reviews that comprehensively provide tools and measurements for assessing the implementation of alcohol control policy. This study reviews tools and measurements for assessing alcohol policy implementation. Policies considered include alcohol pricing and taxation, alcohol marketing control, physical availability control and drink‐driving policy.ApproachWe conducted a scoping review from Scopus, Web of Science and the World Health Organization's website. We included studies on policy implementation for the four most effective prevention policies published worldwide between 2000 and 2021.Key FindingsThe search yielded 11,654 articles and these were narrowed down to 39 included studies. Of these 39 studies, almost half assessed the implementation of a drink‐driving policy (n = 19), followed multipolicy (n = 12) and physical availability control (n = 8). There was no single study assessing policy implementation of pricing and taxation or alcohol marketing control. The majority of the studies were conducted in high‐income countries (n = 31). Globally, there is no standardised tool or guidelines for measuring the policy implementation of these four policies. The tools for measuring policy implementation mostly focused on a single policy, and few covered multiple policies.ImplicationsWe recommend developing standardised tools and measurements to monitor policy implementation across multiple policies at country levels.ConclusionThis review highlighted a lack of comprehensive and standardised tools to assess policy implementation and the limited number of studies on alcohol policy implementation in low‐ and middle‐income countries.
Peripheral neurostimulation for encoding artificial somatosensations
Authors: Valle Giacomo 
Published: September 2022

AbstractThe direct neural stimulation of peripheral or central nervous systems has been shown as an effective tool to treat neurological conditions. The electrical activation of the nervous sensory pathway can be adopted to restore the artificial sense of touch and proprioception in people suffering from sensory‐motor disorders. The modulation of the neural stimulation parameters has a direct effect on the electrically induced sensations, both when targeting the somatosensory cortex and the peripheral somatic nerves. The properties of the artificial sensations perceived, as their location, quality and intensity are strongly dependent on the direct modulation of pulse width, amplitude and frequency of the neural stimulation. Different sensory encoding schemes have been tested in patients showing distinct effects and outcomes according to their impact on the neural activation. Here, I reported the most adopted neural stimulation strategies to artificially encode somatosensation into the peripheral nervous system. The real‐time implementation of these strategies in bionic devices is crucial to exploit the artificial sensory feedback in prosthetics. Thus, neural stimulation becomes a tool to directly communicate with the human nervous system. Given the importance of adding artificial sensory information to neuroprosthetic devices to improve their control and functionality, the choice of an optimal neural stimulation paradigm could increase the impact of prosthetic devices on the quality of life of people with sensorimotor disabilities.
Signs and symptoms of COVID‐19 in patients with multiple sclerosis
Authors: Schiavetti Irene , Carmisciano Luca , Ponzano Marta , Cordioli Cinzia , Cocco Eleonora , Marfia Girolama Alessandra , Inglese Matilde , Filippi Massimo , Radaelli Marta , Bergamaschi Roberto , Immovilli Paolo , Capobianco Marco , De Rossi Nicola , Brichetto Giampaolo , Scandellari Cinzia , Cavalla Paola , Pesci Ilaria , Confalonieri Paolo , Perini Paola , Trojano Maria , Lanzillo Roberta , Tedeschi Gioacchino , Comi Giancarlo , Battaglia Mario Alberto , Patti Francesco , Salvetti Marco , Sormani Maria Pia ,   MuSC‐19 Study Group
Published: September 2022

AbstractBackground and purposeClinical outcomes of multiple sclerosis (MS) patients affected by coronavirus disease 2019 (COVID‐19) have been thoroughly investigated, but a further analysis on main signs and symptoms and their risk factors still needs attention. The objective of this study was to group together and describe based on similarity the most common signs and symptoms of COVID‐19 in MS patients and identify all factors associated with their manifestation.MethodLogistic and linear regression models were run to recognize factors associated with each pooled group of symptoms and their total number.ResultsFrom March 2020 to November 2021, data were collected from 1354 MS patients with confirmed infection of COVID‐19. Ageusia and anosmia was less frequent in older people (odds ratio [OR] 0.98; p = 0.005) and more in smoker patients (OR 1.39; p = 0.049). Smoke was also associated with an incremental number of symptoms (OR 1.24; p = 0.031), substance abuse (drugs or alcohol), conjunctivitis and rash (OR 5.20; p = 0.042) and the presence of at least one comorbidity with shortness of breath, tachycardia or chest pain (OR 1.24; p = 0.008). Some disease‐modifying therapies were associated with greater frequencies of certain COVID‐19 symptoms (association between anti‐CD20 therapies and increment in the number of concomitant symptoms: OR 1.29; p = 0.05). Differences in frequencies between the three waves were found for flu‐like symptoms (G1, p = 0.024), joint or muscle pain (G2, p = 0.013) and ageusia and anosmia (G5, p < 0.001). All cases should be referred to variants up to Delta.ConclusionSeveral factors along with the choice of specific therapeutic approaches might have a different impact on the occurrence of some COVID‐19 symptoms.
Correlation between patient‐reported manual ability and three objective measures of upper limb function in people with multiple sclerosis
Authors: Solaro Claudio , Di Giovanni Rachele , Grange Erica , Brichetto Giampaolo , Mueller Margit , Tacchino Andrea , Bertoni Rita , Patti Francesco , Pappalardo Angelo , Prosperini Luca , Castelli Letizia , Rosato Rosalba , Cattaneo Davide , Marengo Davide 
Published: September 2022

AbstractBackground and purposeUpper limb (UL) function is often affected in people with multiple sclerosis (PwMS) and is typically assessed through objective measures, including the Nine Hole Peg Test (9‐HPT), Box and Block Test (BBT), and Hand Grip Strength (HGS). It is important to include the subjective perspective of PwMS in the assessment. This study aims to evaluate associations between Manual Ability Measure‐36 (MAM‐36) and 9‐HPT, BBT, and HGS in MS.MethodsThe cross‐sectional study included five Italian centers. Inclusion criteria were age ≥ 18 years, MS diagnosis, and stable disease course. Exclusion criteria were bilateral UL paralysis, and concomitant orthopedic or neurological diseases.ResultsA total of 199 PwMS were included: 128 female, mean age = 50.7 ± 13.0 years, 119 relapsing–remitting MS (RRMS), 31 primary and 49 secondary progressive MS, mean disease duration = 14.0 ± 10.4, years, mean Expanded Disability Status Scale (EDSS) = 4.6 ± 2.0.The MAM‐36 showed small correlations with 9‐HPT, BBT, and HGS. Correlations between MAM‐36 and 9‐HPT and BBT were highest among subjects with EDSS ≥ 6 and progressive MS. MAM‐36 and HGS showed the highest correlations in subjects with EDSS ≤ 5 and RRMS. Combining 9‐HPT and HGS provided the strongest predictive power over the MAM‐36.ConclusionsCorrelations between objective measures and MAM‐36 were small to moderate, meaning that objective measures do not match subjects' perception of UL function. The combination of 9‐HPT and HGS measures can help improve the assessment of UL function in activities of daily living.
Value preferences of spouses and division of domestic labor in the family: A dyadic study
Authors: Tartakovsky Eugene 
Published: September 2022

AbstractObjectiveThis study investigated the effect of the spouses' value preferences on the division of domestic labor in the family.BackgroundPersonal value preferences reflect general motivations and thus affect individuals' perception of reality and direct behavior. In the present study, I assumed that the personal value preferences of spouses motivate them to participate in different domains of domestic labor to varying degrees.MethodThe study was conducted in Israel using a sample of heterosexual couples having at least one child (N = 479). I measured the value preferences of the spouses and division of domestic labor in the family in four domains: traditionally female chores, traditionally male chores, childcare, and emotion work. Dyadic data analysis was conducted using structural equation modeling.ResultsAmong husbands, a higher preference for the self‐transcendence values was associated with a more equal division of domestic labor in the family; that is, with a larger share of husbands in traditionally female chores, childcare, and emotion work, and with their smaller share in traditionally male chores. Among wives, higher preferences for openness to change and self‐enhancement values were associated with a more equal division of domestic labor in the family.ConclusionThe results confirmed that the spouses' general motivational goals expressed in their value preferences affected their participation in different domains of domestic labor.ImplicationsThe study advances researchers' and practitioners' understanding of the intrapersonal motivational factors affecting the division of domestic labor and provides a solid basis for further research and counseling work with families.
Are separated/divorced same sex families more likely to establish equal coparenting? A preliminary outcome from a court‐based program
Authors: Zhu Tianmei , Alschech Jonathan , Pruett Marsha Kline 
Published: September 2022

AbstractIn this article, we examine the impact of inconsistent laws and possible judicial bias on children's opportunities to experience interaction and support from all parents, when those same parents separate/divorce. The Family Resolutions Specialty Court (FRSC) in Massachusetts provides a model through which procedures support all parents and minimize bias by engaging parental involvement collaboratively. Using exploratory data collected and analyzed from separated/divorced same sex and heterosexual parenting plans developed by families in FRSC (N = 50; 86% heterosexual, 14% same sex), we examine children's living arrangements regarding daytime parenting time and the number of overnights with the non‐primary parent, as well as the consistency of parenting schedules. Results identified a tendency among same sex parents to establish parenting plans that were more equal between partners and consistent across weeks. This small study adds to the emerging literature exploring coparenting among same sex parents post‐separation/divorce by contextualizing this preliminary finding and setting the stage for more rigorous analysis of differences in parenting plans and coparenting post separation/divorce between heteronormative and queer families.