bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model
Authors: Paul Sudip , Sone Masamitsu , Patil Ashwini , Kato Hisaya , Maezawa Yoshiro , Oshima Motohiko , Fukuyo Masaki , Rahmutulla Bahityar , Ouchi Yasuo , Tsujimura Kyoko , Nakanishi Mahito , Kaneda Atsushi , Iwama Atsushi , Yokote Koutaro , Eto Koji , Takayama Naoya 
Published: May 2022

Abstract

The underlying mechanisms of the second leading cause of death among Werner syndrome (WS) patients, atherosclerosis, is not fully understood. Here, we established an in vitro co-culture system using macrophages (iMφs), vascular endothelial cells (iVECs), and vascular smooth muscle cells (iVSMCs) derived from induced pluripotent stem cells. WS-iMφs induced endothelial dysfunction and characteristics of phenotype switching in WS-iVECs and WS-iVSMCs, respectively. RNA-seq and ATAC-seq revealed accelerated activation of type I interferon signaling and reduced chromatin accessibility of several transcriptional binding sites required for cellular homeostasis in WS-iMφs. Furthermore, we found higher expression of retrotransposable elements in WS-iMφs and WS peripheral blood-derived-Mφs and evidence that retrotransposable element-derived dsRNA may activate the DHX58-dependent cytoplasmic RNA sensing pathway in WS-iMφs. Conversely, silencing type I interferon signaling in WS-iMφs suppressed cellular senescence and inflammation. These findings suggest that Mφ-specific inhibition of type I interferon signaling could be targeted to treat atherosclerosis in WS patients.
Teenage Girls’ Interpersonal Communication with Mothers after Experiencing Menarche in Bangladesh
Authors: Onia Maksuda Akter , Zakaria Muhammad , Jahan Nusrat , Akter Dilruba , Zhou Yuyin , Cheng Feng , Xu Junfang 
Published: May 2022

Abstract
Background
In Bangladesh, particularly in rural areas, it is not common for teenage girls to openly discuss sexual and reproductive health (SRH) issues including menarche with their mothers because of cultural taboos and traditional beliefs. In light of this, we aim to explore mother-daughter interpersonal communication in regards to menarche and influencing determinants in Chattogram, Bangladesh.
Methods
A cross-sectional questionnaire survey was conducted among younger adolescent girls (N = 870) attending grade 7 and grade 8 aged 12–14 years in six high schools. In addition, mothers were also interviewed to obtain more in-depth insights into the issue.
Results
Among adolescents, 81.6% communicated with their mothers followed by sisters, relatives and friends after experiencing menarche, while 59.9% reported that it was mothers who first commenced interaction with them. More than half (51.7%) stated that they felt shy during interaction with their mothers after menarche. Many mothers transmitted different myths and misconceptions with regards to menstruation, with this more prevalent in rural areas compared to urban settings. Being urban respondents, use of Facebook by mothers, mothers as the prime source of SRH knowledge, and prior knowledge of menarche were reported as the significant predictors (p < 0.05) for the extent of communication between adolescents and mothers.
Conclusion
The type of communication regarding menarche displayed an unhealthy interactional process and behavioral pattern. Therefore, providing more detailed and comprehensive SRH education in the school curriculum, developing and distributing behavioral change communication materials among mothers and adolescents are urgently required.
Exogenous Salicylic Acid Enhanced Resistance of Foxtail Millet (Setaria italica) to Sclerospora graminicola
Authors: Hou Shupei , Liu Zhaowen , Li Yaqiong , Yang Mingzhen , Hou Siyu , Han Yuanhuai , Zhao Yaofei , Sun Zhaoxia 
Published: May 2022

Abstract
To ward off pathogens, plants establish innate immunity system in which salicylic acid (SA) plays an important role. The downy mildew of foxtail millet (Setaria italica) is an oomycete disease caused by biotrophic pathogen Sclerospora graminicola, which seriously affects the yield and quality of foxtail millet. However, it is not clear how SA regulating the defense responses of foxtail millet. In this study, we found that spraying exogenous SA of low concentration (1 mM and 3 mM) had good effect on enhancing the resistance while high concentration (6 mM and 9 mM) did not significantly affect the defense of foxtail millet. Excess SA inhibited the growth and development of plants and we found spraying exogenous SA reduced the plant height, fresh weight and dry weight of foxtail millet. The low concentration of exogenous SA promoted the accumulation of Chlorophyll, soluble sugar and proline, promoted the activity of Phenylalanine Ammonia-Lyase and suppressed the accumulation of Malondialdehyde. After the treatment with a suitable concentration of SA, the expression of related to disease resistance was increased at the early stage after treatment. The results indicated exogenous SA could enhance the resistance of foxtail millet and provided a basis for improving the control methods of downy mildew.
Prediction model of delayed graft function based on clinical characteristics combined with serum IL-2 levels
Authors: Zhao Shitao , Liu Yuan , Zhou Cheng , Chen Zide , Cai Zeyu , Han JiaLiang , Xiao Jiansheng , Xiao Qi 
Published: May 2022

Abstract
Background
Kidney transplantation is an effective treatment for end-stage renal disease (ESRD). Delayed graft function (DGF) is a common complication after kidney transplantation and exerts substantial effects on graft function and long-term graft survival. Therefore, the construction of an effective model to predict the occurrence of DGF is particularly important.
Methods
Seventy-one patients receiving their first kidney transplant at the First Affiliated Hospital of Nanchang University from October 2020 to October 2021 were enrolled in the discovery cohort. Based on clinical characteristics and serum markers, a logistic regression model was used to simulate the risk of DGF in the discovery cohort. The DGF prediction model was named the prediction system and was composed of risk factors related to DGF. Thirty-two patients receiving a kidney transplant at the First Affiliated Hospital of Nanchang University from October 2021 to February 2022 were enrolled in the validation cohort. The validation cohort was used to verify the accuracy and reliability of the prediction model.
Results
Cold ischemia time (CIT), donor history of diabetes mellitus, donor interleukin-2 (IL-2) level and donor terminal creatinine level constitute the prediction system. In the validation test, the area under the receiver operating characteristic curve (AUC) was 0.867 for the prediction system, and good calibration of the model was confirmed in the validation cohort.
Conclusions
This study constructed a reliable and highly accurate prediction model that provides a practical tool for predicting DGF. Additionally, IL-2 participates in the kidney injury process and may be a potential marker of kidney injury.
Perceptions of barriers to and facilitators of cardiac rehabilitation in healthcare providers and policy stakeholders: a questionnaire survey
Authors: Kim Chul , Kwak Hae-Bin , Sung Jidong , Han Jae-Young , Lee Jang Woo , Lee Jong Hwa , Kim Won-Seok , Bang Heui Je , Baek Sora , Joa Kyung Lim , Kim Ae Ryoung , Lee So Young , Kim Jihee , Kim Chung Reen , Kwon Oh Pum , Sohn Min Kyun , Moon Chang-Won , Lee Jae-In , Jee Sungju 
Published: May 2022

Abstract
Background: In Korea, cardiac rehabilitation is poorly established in hospitals and communities. Therefore, this study aimed to determine the perceptions of the need for cardiac rehabilitation programs and relevant health policies among hospital directors and health policy managers.Methods: We sent a questionnaire to 592 public health policy managers and directors of selected hospitals, 132 of whom returned a completed questionnaire (response rate: 22.3 %). The participants were categorized into five types of organizations depending on their practice of PCI(Percutaneous Coronary Intervention), establishment of cardiac rehabilitation, director of hospital, and civil servant. The responses were analyzed using a Kruskal-Wallis test. Differences in the opinions between directors of hospitals that perform/do not perform PCI, directors of hospitals with/without cardiac rehabilitation, and between hospital directors and health policy managers were analyzed with Mann-Whitney tests for post-hoc comparisons. Results: Responses about targeting diseases for cardiac rehabilitation, patients’ roles in cardiac rehabilitation, hospitals’ roles in cardiac rehabilitation, and governmental health policies’ roles in cardiac rehabilitation were more positive among hospitals that perform PCI than those that do not. Responses to questions about the effectiveness of cardiac rehabilitation and hospitals’ roles in cardiac rehabilitation tended to be more positive in hospitals with cardiac rehabilitation than in those without. Hospital directors responded more positively to questions about targeting diseases for cardiac rehabilitation and governmental health policies’ roles in cardiac rehabilitation than civil servants, and both hospitals and public organizations provided negative responses to the question about patients’ roles in cardiac rehabilitation. Responses to questions about targeting diseases for cardiac rehabilitation, patients’ roles in cardiac rehabilitation, and governmental health policies’ roles in cardiac rehabilitation were more positive in hospitals that perform PCI than those that do not and public organizations.Conclusions: Hospitals must ensure timely referral, provide education, and promote the need for cardiac rehabilitation. In addition, governmental socioeconomic support is needed for incentives, nighttime cardiac rehabilitation programs, and continuum of service into community-based programs.
A Study on lubrication and cooling performance and machining characteristics of magnetic field assisted minimum quantity lubrication used Fe3O4 nanofluid as cutting fluid
Authors: Lv Tao , Xu Xuefeng , Weng Haizhou , Yu Aibing , Niu Chengcheng , Hu Xiaodong 
Published: May 2022

Abstract
As an effective alternative to traditional pouring lubrication method, minimum quantity lubrication (MQL) has been recognized by more and more scholars and applied in factories. However, there are still some technical shortcomings in the application of traditional oil-based MQL technology, such as poor cooling capacity and low lubrication efficiency. Aiming at the technical defects of oil-based MQL, a magnetic field assisted minimum quantity lubrication (mMQL) technology used Fe3O4 nanofluid as cutting fluid was proposed. Firstly, MQL equipment with controllable magnetic field was built, and the contact angle, surface tension and dynamic viscosity of Fe3O4 nanofluid droplets were measured under different magnetic induction intensity. Based on these variation parameters of physical properties of the magnetic nanofluid droplets, the influence of magnetic induction on the droplet penetrability was subsequently analyzed. Finally, the heat transfer performance and machining characteristics of the nanofluid droplets under different magnetic induction were compared and investigated. Results showed that the Fe3O4 nanofluid droplets exhibited lower contact angle and higher viscosity under the influence of magnetic field, thus showing better penetrability and heat transfer performance. Compared with traditional vegetable oil MQL, the cutting temperature, tool wear and cutting force under mMQL were reduced by ~ 35.5%, ~ 52.4% and ~ 43.2%, respectively. Under the influence of magnetic field, the contact angle of magnetic droplets was decreased, and the contact area between the droplets and heat transfer surface was larger, which led to the improvement of droplet evaporation heat transfer efficiency. In addition, the penetrability of Fe3O4 nanofluid assisted by magnetic field was improved, more nanofluid could penetrate into the tool-chip contact area, and finally improved its lubrication and cooling efficiency.
Origanum vulgare prevents kaolin–induced hydrocephalus via regulation of GFAP and lba1 proteins expression in the rat brain
Authors: Vahabzadeh Gelareh , Moazam Ashrafsadat , Rahimi Mandana , Khajehasani Fatemeh , Motevalian Manijeh 
Published: May 2022

Abstract
Background:The reactive gliosis contributes to patterns of hydrocephalus injury and recovery. The study aimed at evaluating Origanum vulgare essential oil (OEO) effects on reactive astrogliosis and reduced lateral ventricle thickness in kaolin-induced hydrocephalic rats. Methods and Results:Thirty male Wistar rats received kaolin via a direct injection into the cisterna magna and compared to control animals. After 21 day post-injection of kaolin, the hydrocephalic rats were randomly divided to four groups and experimental groups underwent magnetic resonance imaging to determine the lateral ventricle volume. Rats received different doses of OEO for 10 days. The lateral ventricular dilatation was evaluated by MRI. Also to assess the Iba-1 and GFAP proteins’ expression level, immunohistochemistry test and western blot analysis were performed 10 days after the injection. The results showed lateral ventricular size in the OEO-treated hydrocephalic rats was smaller than that of those receiving kaolin without OEO. The hydrocephalic rats increased reactive astrocytes and reactive microglia compared to the OEO-treated animals based on GFAP and Iba-1 immunoreactivity results. The findings were confirmed by western blot. There were significant differences between the hydrocephalus control and the 200 mg/kg/day OEO-treated rats in terms of GFAP and Iba-1 expression levels. Conclusions:In nonclusions, the OEO treated groups had stabilized ventricular expansion compared to hydrocephalus rats. Findings of the study showed that OEO can restrain the hydrocephalus-associated damage in rat brain induced by kaolin, likely by reducing GFAP and Iba-1 and therefore the OEO intervention, might be useful as a new treatment strategy.
Multiple MicroRNAs are involved in regulating peanut (Arachis hypogaea L.) resistance to Sclerotium rolfsii at the early stage
Authors: Xu Yongju , Zhang Xiaojun , Hou Rui , Zhang Xiaohong , Li Shuang , Yue Fuliang , Zhang Xiangqiong , Zhu Xunlu 
Published: May 2022

Abstract
Stem rot, caused by soilborne pathogen Sclerotium rolfsii, is one of the most destructive diseases of peanut (Arachis hypogaea L.) worldwide. Although microRNAs (miRNAs) are indispensable regulators for plant defense, the miRNA species have not been explored for peanut immunity against the soilborne pathogen Sclerotium rolfsii. Here, we report a miRNA comparative analysis of the durably resistant peanut variety Rizhaohua-1 (Rzh) and the susceptible peanut variety Jitian-1 (JT) in response to infection by Sclerotium rolfsii. We identified a group of known and novel miRNAs that were differentially expressed upon S. rolfsii infection. The predicted target genes included receptor kinases, transcription factors, and genes involved in the production and transport of metabolites. In this study, we have shown that miRNAs regulate a broad range of genes to respond to the pathogen, and eventually establish a systemic defense network to combat disease.
Excellent removal of knob-into-hole bispecific antibody byproducts and impurities in a single capture chromatography
Authors: Chen Serene W , Hoi Kong Meng , Mahfut Farouq Bin , Yang Yuansheng , Zhang Wei 
Published: May 2022

Abstract
Bispecific antibodies (bsAbs) are therapeutically promising due to their ability to bind to two different antigens. However, the bsAb byproducts and impurities, including mispaired homodimers, half antibodies, light chain mispairings, antibody fragments and high levels of high molecular weight (HMW) species, all pose unique challenges to their downstream processing. Here, using two knob-into-hole (KiH) constructs of bsAbs as model molecules, we demonstrate the excellent removal of bsAb byproducts and impurities in a single Protein A chromatography under optimized conditions, including hole-hole homodimer mispaired products which are physicochemically very similar to the target bsAbs and still present even with the use of the KiH format, though at reduced levels. The removal occurs through the incorporation of an intermediate low pH wash step and optimal elution conditions, achieving ~ 60% improvement in monomeric purity in a single Protein A step, without the introduction of sequence specific bsAb modifications to specifically induce differential Protein A binding. Our results also suggest that the higher aggregation propensity of bsAbs may cause aggregation during the column process, hence an optimisation of the appropriate loading amount, which may be lower than that of monoclonal antibodies (mAbs), is required. With the use of loading at 50% of 10% breakthrough (QB10) at 6 min residence time, we show that an overall high monomer purity of 92.1–93.2% can be achieved with good recovery of 78.4–90.6% within one capture step, which is a significant improvement from a monomer purity of ~ 30% in the cell culture supernatant (CCS). The results presented here would be an insightful guidance to all researchers working on the purification process development to produce bispecific antibodies, especially for knob-into-hole bispecific antibodies.
A pentaploid endosperm and a Penaea-type embryo sac are likely synapomorphies of Azorella (Apiaceae, Azorelloideae)
Authors: Ptáček Jan , Sklenář Petr , Pinc Jan , Urfusová Romana , Calviño Carolina I. , Urfus Tomáš 
Published: May 2022

Abstract
About 80% of angiosperms form a monosporic Polygonum-type embryo sac whereas in the remaining species eleven other types of embryo sac are found. Evidence as to the type of embryo sac is lacking for many plant species, and the role of higher-ploidy endosperm is unknown. In contrast to the rest of the Apiaceae, where a Polygonum-type embryo sac (3n endosperm) has been reported, the few species of the Azorelloideae studied form a Drusa-type embryo sac (3n endosperm) or a Penaea-type embryo sac (5n endosperm). This variation within Azorelloideae makes this subfamily, and its genus Azorella in particular, a good candidate for studying the evolutionary importance of the embryo sac and endosperm in diversification. We studied the variation in the type of embryo sac and the ploidy level of the endosperm in Andean-Patagonian Azorella and closely related Pozoa on a sample of 101 individuals from 31 populations of 21 species. We employed flow cytometric seed screening and calibrated the results of ploidy level estimation against embryological observations. In addition, we examined the genome size variation of the species sampled. All species of Azorella formed Penaea-type embryo sacs and a pentaploid endosperm whereas one species of Pozoa formed triploid and the other tetraploid endosperms. Variations in the type of embryo sac and endosperm ploidy have probably shaped the evolution of the different lineages of Azorelloideae in the southern Andes. A Penaea-type embryo sac, which represents a likely synapomorphy of Azorella, is a feature of underestimated significance in the evolution of angiosperms.
miR-451a-5p/miR-34a-5p restoration could suppress human breast cancer cell proliferation and migration through Wnt/β- catenin and ERK/P-ERK signaling pathways
Authors: Khordadmehr Monireh , Jigari-Asl Farinaz , Baradaran Behzad , Baghbani Elham , Noorolyai Saeed , Rahmani Shima 
Published: May 2022

Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs of 21–25 nucleotides in length and display an essential role in regulating cancer initiation, development, and progression. In this study, the impacts of microRNA-451a-5p and miR-34a-5p (tumor suppressors), individually and combined, transfections were conducted on the apoptosis, proliferation, and migration of breast cancer cells in vitro. For carrying out this research, malignant breast cancer cells (MDA-MB-231) were transfected with miR-451a-5p and miR-34a-5p mimics. Then, apoptotic rate, proliferation, and migration of cancer cells were assessed by MTT (cell viability), flow cytometry (apoptosis and cell cycle arrest), q-RT-PCR (expression levels of caspase-3, caspase-8, MMP9, ROCK, vimentin, c-Myc genes), wound healing, and western blot (protein expression of β- catenin, ERK, and P-ERK) assays. Our findings indicated that miR-34a-5p and miR-451a-5p could additionally induce apoptosis and cell cycle arrest in the sub-G1-phase, repress the proliferation and migration in the breast cancer cells, and could also decrease β- catenin, ERK/P-ERK protein expressions. Taken to gather, miR-451a and miR-34a have a considerable role in breast cancer cell proliferation and migration ability via Wnt/β-catenin and ERK/P-ERK signaling pathways. Therefore, the simultaneous restoration of the presented tumor suppressor miRNAs may be proposed as a valuable and potential therapeutic strategy in breast cancer treatment. However, further study should be meaningful.
Comparison of mitochondrial genetic variation of Taenia hydatigena cysticerci from China and Mongolia
Authors: Qurishi Sayed Ajmal , Yan Hong-Bin , Li Li , Ohiolei John Aeskhaen , Alvi Mughees Aizaz , Zhang Lin-Sheng , Da Ha , Qiao Hong-Mei , Shumuye Nigus Abebe , Hua Bao , Bai Bing-Xin , Tian Wen-Jun , Xu Ju-Mei , Fu Bao-Quan , Jia Wan-Zhong 
Published: May 2022

Abstract
Parasitic infection is one of the many challenges facing livestock production globally. Cysticercosis tenuicollis is a common parasitic disease in domestic and wild ruminants (intermediate host) caused by the larval stage of Taenia hydatigena primarily through infecting dogs (definitive host). Although there are handful of genetic studies on this parasite, there is no available genetic data for the parasite in Mongolia. So, the main aim of this study was to identify the nuclear differences in ovine isolates of Cysticercus tanuicollis entering China from Mongolia and comparison with existing Chinese isolates from sheep and goats based on the recently described PCR-RFLP method and mitochondrial genes of NADH dehydrogenase subunit 4 (nad4) and the NADH dehydrogenase subunit 5 (nad5). Sixty nine isolates were collected during routine veterinary meat inspections from sheep that originated from Mongolia, at the modern Slaughterhouses in Erenhot City, Inner Mongolia. Additional 114 cysticerci were also retrieved from sheep and goats from northern (Inner Mongolia Autonomous Region, Ningxia Hui Autonomous Region and Gansu Province), western (Tibet Autonomous Region), and southern (Jiangxi Province and Guangxi Province) China. The PCR-RFLP approach of the nad5 showed nine mitochondrial clusters such as A1, A2, A3, A5, A8, A9, A10, A11 and B of T. hydatigena isolates from sheep and goats from Mongolia and China. Meanwhile, RFLP profile of Haplogroup A1 was extensively spread than other Haplogroups. Therefore, these data provide supplementary information on the molecular epidemiology of T. hydatigena for China and Mongolia as animal movement between both countries is constantly occurring. This study also demonstrates that the genetic variants of T. hydatigena existing in China and Mongolia are closely similar.