bluuebunny/crossref_metadata_2025_split huggingface dataset:
Femoral Rollback at High-Flexion During Squatting is Related to the Improvement of Sports Activities After Bicruciate-Stabilized Total Knee Arthroplasty: An Observational Study
Authors: Kono Kenichi , Inui Hiroshi , Kage Tomofumi , Tomita Tetsuya , Yamazaki Takaharu , Taketomi Shuji , Yamagami Ryota , Kawaguchi Kohei , Tanaka Sakae 
Published: February 2022

Abstract
Background
In bicruciate-stabilized total knee arthroplasty (BCS-TKA), the relationship between the postoperative kinematics and sports subscales in patient-reported outcome measures (PROMs) remains unknown. The purpose of this study was to determine the relationship between kinematics and sports subscales using the PROMs after BCS-TKA.
Methods
Sixty-one patients with severe knee osteoarthritis were examined at 13.5 ± 7.8 months after BCS-TKA. The patients performed squattings under single fluoroscopic surveillance in the sagittal plane. Range of motion of the knee, axial rotation of the femur relative to the tibial component, and anteroposterior (AP) translation of the medial and lateral femorotibial contact points were measured using a 2D-to-3D registration technique. In addition, the relationship between the kinematics and improvement of the sports subscales in the Knee Injury and Osteoarthritis Outcome Score (KOOS) was evaluated.
Results
In rotation angle, the femoral external rotation was observed from 0–50° of flexion. The amount of femoral external rotation did not correlate with PROMs-SP. In medial AP translation, posterior translation was observed from 0–20° and 80–110° of flexion. The mild anterior translation was observed from 20–80° of flexion. Beyond 80° of flexion, posterior translation was positively correlated with squatting. In lateral AP translation, posterior translation was observed from 0–20° and 80–110° of flexion. Beyond 80° of flexion, posterior translation was positively correlated with running, jumping, twisting/pivoting, and kneeling.
Conclusion
Femoral rollback at high flexion during squatting is related to the improvement in sports activities after BCS-TKA.
Individualized Enhanced recovery after surgery (ERAS) protocols for patients with hepatocellular carcinoma: a retrospective study
Authors: Zhang Yu , Cheng Xin , Zhang Lei , Xu Zhongling , Feng Zihan , Chen Zeyin , Chen Zhong 
Published: February 2022

Abstract
Background:Hepatocellular carcinoma (HCC) patients often undergo curative liver resection to treat this form of cancer. Hepatectomy is, however, a form of major surgery associated with many significant risks including prolonged hospitalization, high costs, impaired physiological function, and high postoperative complication rates. Enhanced recovery after surgery (ERAS) is a multidisciplinary approach that seeks to expedite postoperative recovery in patients undergoing major surgeries in order to lower postoperative complication rates. Prior studies have successfully employed ERAS approaches in the context of gynecological, urological, and cardiovascular surgeries. However, HCC is a complex disease and affected patients may also suffer from pre-existing liver disease, making it essential that they be administered appropriate individually tailored treatments. This study was thus designed to assess the efficacy and safety of individualized ERAS approaches in patients undergoing hepatectomy. Methods:In total, we retrospectively analyzed data from 90 HCC patients that underwent hepatectomy between October 2018 and August 2019. All patients met the study enrollment criteria and provided written informed consent to participate. All studies were approved by the Hospital Research Ethics Committee and were consistent with the Declaration of Helsinki. Patients were randomly divided into two groups (n=45 each) based on the employed perioperative treatment strategies: a conventional treatment group and an ERAS treatment group. Key outcomes were then compared between groups, including postoperative pain scores, duration of postoperative hospitalization, medical costs, and rates of readmission. Quantitative data are given as `x±s and were compared via Student’s t-tests, whereas categorical data were compared via chi-squared tests and Fisher's exact test.Results:ERAS treatment was associated with lower postoperative pain scores at 24, 48, and 72 h post-treatment (P<0.05), with a shorter postoperative hospitalization duration (8.16 days vs.10.49 days; P<0.004), and with lower medical costs (P<0.004) as compared to traditional treatment. No significant differences in complication rates (P>0.05) or readmission rates (P>0.557) were observed between these groups. Conclusions:Individualized ERAS improves patient postoperative recovery more effectively than traditional treatment in patients undergoing hepatectomy.
Thermal and Chemical Exhaust Gas Recirculation Potential of Punnai Oil Biodiesel Fuelled Diesel Engine For Environmental Sustainability
Authors: Bibin Chidambaranathan , Devan Ponnusamy Kumarasami , Sheeja Raghavan , Madhu Sundaram 
Published: February 2022

Abstract
Pollution from diesel engines affects negatively the environment. As a result, there is a worldwide concern about reducing the pollutants emitted by diesel engines. In comparison to diesel fuel, biodiesel combustion produces reduced carbon monoxide (CO2) and unburned hydrocarbon (UHC) emissions, but higher nitrogen oxides (NOx) emissions. The current study is to investigate the thermal and chemical effect of exhaust gas recirculation (EGR) on features of a diesel engine for environmental sustainability. The punnai oil was produced from kernels of punnai seeds and transesterified in two phases using alcohol with the existence of a catalyst. The higher viscosity of punnai oil biodiesel is diluted by mixing it with diesel fuel. Our previous investigation indicated that neat punnai oil biodiesel is a potential fuel; however, the findings showed that the addition of diesel is necessary to obtain acceptable engine performance. In this study, punnai oil biodiesel was mixed at a rate of 20% with diesel (B20) and run in a diesel engine with varied EGR rates under five different engine loads. This combined impact enhanced the maximum heat release rate (HRR) and maximum combustion pressure, according to the findings. The premixed burning fractions were commonly higher at all engine loads, whereas the diffusion combustion fractions were lower. When the centre of the HRR changed toward the top dead centre (TDC), combustion durations remained rather constant. The experimental results revealed B20 blend at 10% EGR flow rate produced 6.57% lower BTE, 37.04% higher BSEC, 2.47% higher EGT, 5.13% lower CO, 31.11% higher CO2, 3.13% higher UHC, 8.36% lower NOx and 4% higher smoke opacity when compared with diesel in a standard diesel engine.
Does the experience of the first assistant affect organ injuries in laparoscopic hysterectomy for benign diseases?
Authors: TSUZUKI YOKO , Hirata Takumi , Tsuzuki Shinya , Wada Shinichiro , Tamakoshi Akiko 
Published: February 2022

Abstract
Purpose: This study sought to explore whether the experience level of the first assistant surgeon influences perioperative organ injuries (ureteral, bladder, and intestinal injuries) in patients undergoing total laparoscopic hysterectomy (TLH) for benign diseases. We defined an experienced surgeon as a surgeon certified by the Skill Qualification Committee of the Japan Society of Gynecologic and Obstetric Endoscopy and Minimally Invasive Therapy or a surgeon with equivalent surgical skills.Methods. We reviewed our surgical registry database of TLH for benign indications between 2014 and 2020 and only selected cases performed by an experienced primary surgeon. Patients were divided into two groups based on the experience level of the first assistant. Inverse probability of treatment weighting by propensity score, which was adjusted for patient and procedure characteristics, was used to examine differences in perioperative organ injuries according to the experience level of the first assistant.Results. Among 1,682 patients who underwent TLH, 18 organ injuries were found (0.83%). In the propensity score inverse probability of treatment weighting models, less experience of the first assistant had no significant impact on the occurrence of perioperative organ injuries (p = 0.348).Conclusion. In TLH for benign indications at our hospital, given an experienced primary surgeon, the inclusion of a less experienced first assistant does not negatively affect the occurrence of perioperative organ injuries.
A Novel Nonsense Mutation(c.1499C&gt;G) in CRB1 Caused Leber Congenital Amaurosis-8 in a Chinese Family and Literature Review
Authors: Duan Wenhua , Zhou Taicheng , Jiang Huawei , Zhang Minhui , Hu Min , Zhang Liwei 
Published: February 2022

Abstract
Background:Leber’s congenital amaurosis (LCA) is a severe hereditary retinopathy disease that is characterized by early and severe reduction of vision,nystagmus,sluggish or absent pupillary responses. To date, the pathogenesis of LCA remains unclear,and the majority cases are caused by autosomal recessive inheritance. In this study, we explored the mutation in the Crumbs homologue 1(CRB1) gene in a Chinese family with LCA. Methods:We conducted comprehensive ocular examinations and collected 5 ml of blood samples from members of a Chinese family with LCA. The pathogenic gene was identified by capturing and sequencing the related genes of ocular diseases.Results: We found a nonsense mutation(c.1499C>G) in the 6th exon of CRB1 in a Chinese family with LCA,which predicted a change of the protein p.S500X,may lead to loss of gene function.Conclusions: This study reported a novel mutation(c.1499C>G,p.S500X) of the CRB1 gene occurred in a Chinese family with LCA, thus expanding the spectrum of CRB1 mutations causing LCA. And we summarize the 76 mutations reported so far in CRB1 that caused LCA8.
Environmental coastal management for groundwater resources using different aquifer bed slopes considering sea level rise risk
Authors: Abd-Elaty Ismail , Polemio Maurizio 
Published: February 2022

Abstract
Todays, coastal groundwater management is a challenging research topic due the relevance of these water resources and the huge suffered risks due to the global change in the coastal areas. The geometrical features of coastal aquifers play a control role on salt water intrusion (SWI). Seawater level rise (SLR), groundwater over pumping and aquifer recharge reduction promoting SWI. All these key factors are considered are considered with two different numerical approaches defining schematic management criteria. The former approach adopts the well-known Henry's problem; the latter is based on the real study case of the Gaza aquifer (Palestine). Different aquifer bed slopes, hydraulic and physical methods, including physical subsurface barriers, earth fill, check dam, abstraction, and natural or artificial recharge are considered together with SLR, recharge reduction, and over pumping. The different scenarios are compared in terms of position of saltwater intrusion interface, measured as distance from the coastline at the aquifer bottom using the numerical code SEAWAT. Useful comparing tables and considerations are defined with the purpose to guide the preliminary selections of new management solutions for reducing the effect of the global change on coastal groundwater resources around the coastal world. The land side bed slope cases show more SWI than sea side and horizontal bed slopes. Cut-off walls and check dams are effective technique to manage SWI in horizontal bed aquifers slopes more than other slopes, also the subsurface dams, earth fill and recharge of freshwater are good methods to control the SWI in land bed slopes while abstraction of brackish water and combine of freshwater recharge with brackish water abstraction are better to mitigate of SWI in sea side slopes.
The genetic consequences of captive breeding, environmental change and human exploitation in the endangered peninsular pronghorn
Authors: Klimova Anastasia , Sánchez-Sotomayor Victor , Rivera Jesus Gutiérrez , Hoffman Joseph Ivan 
Published: February 2022

Abstract
Endangered species with small population sizes are susceptible to genetic erosion, which can be detrimental to long-term persistence. Consequently, monitoring and mitigating the loss of genetic diversity are key to successful conservation. The peninsular pronghorn (Antilocapra americana peninsularis) is an endangered pronghorn subspecies that is almost entirely held in captivity. Captive breeding has increased the number of pronghorns from 25 founders in 1997 to around 700 individuals today, but it is unclear how the genetic diversity of the captive herd may have changed over time. We therefore generated and analysed microsatellite data spanning 2009–2021. We found a decline in heterozygosity and an increase in the proportion of inbred individuals over time. However, these trends appear to have abated in response to a genetically informed selective breeding attempt undertaken in 2018. We also reconstructed the recent demographic history of the peninsular pronghorn, revealing two sequential population declines putatively linked to the desertification of the Baja California peninsula around 6,000 years ago, and hunting and habitat loss around 500 years ago. Our results provide insights into the genetic diversity of an endangered antelope and highlight the potential for selective breeding to have positive conservation outcomes.
Recurrent Torsion of Otherwise Normal Adnexa: Oophoropexy Does Not Prevent Recurrence
Authors: Smorgick Noam , Mor Matan , Eisenberg Neta , Dovev Maya Naor , Vaknin Zvi 
Published: February 2022

Abstract
Purpose
Recurrence of adnexal torsion involving otherwise normal adnexa is not rare. Various oophoropexy (ovarian fixation) procedures have been suggested to prevent recurrence, however, long-term information of their efficacy is lacking. The aim of this study was to investigate the recurrence rate of adnexal torsion following oophoropexy.
Methods
Retrospective cohort study, including all consecutive patients who underwent an oophoropexy procedure for the prevention of recurrent torsion of “normal adnexa” in our department from 2008 to 2021. Oophoropexy techniques included shortening of the utero-ovarian ligament (between 2008 to 2019) or combined utero-ovarian and round ligament fixation (from 2020).
Results
Twenty-one patients (age range 7-35 years) with a mean follow-up of 82.7 ± 60.6 months were identified. Fifteen of them (71.4%) were re-operated for recurrent torsion following an oophoropexy procedure, while six (28.6%) did not experience recurrence. Twelve (57.1%) torsion recurrences following an oophoropexy occurred within the first 2 postoperative years. There were no differences in mean age and menarchal status )pre- or post-menarchal) at the time of the first torsion event, age at the time of oophoropexy, oophoropexy side, number of adnexal torsion events before oophoropexy, and follow-up duration between those with and those without post-oophoropexy recurrences.
Conclusion
Oophoropexy procedures may not prevent recurrent torsion of otherwise normal adnexa. Further studies to determine whether combined fixation (utero-ovarian plication and round ligament) is more efficacious than isolated utero-ovarian plication for the prevention of recurrent torsion are warranted.
Potential Predictive Factors for Clinical Outcomes Following Progressive Pain Reduction Associated With Conservative Treatment for Recently Diagnosed Osteoporotic Vertebral Fractures: A Case Control Study
Authors: Shibao Yosuke , Koda Masao , Takeuchi Yosuke , Asada Tomoyuki , Sato Kosuke , Kono Mamoru , Eto Fumihiko , Miura Kousei , Noguchi Hiroshi , Takahashi Hiroshi , Funayama Toru , Tatsumura Masaki , Yamazaki Masashi 
Published: February 2022

Abstract
IntroductionThe purpose of the present study was to elucidate a potential predictive factor for poor clinical outcomes in the conservative treatment of osteoporotic vertebral fractures.Materials and MethodsThis is a study of the vertebrae of 41 patients selected from 63 patients with recent osteoporotic fractures admitted to our hospital from April 2013 to March 2015. Patients with dementia were excluded. We analyzed visual analog scale (VAS: 0‒100 mm) scores and dynamic radiographic images over time for up to 12 weeks after admission. Our statistical analyses were designed to identify factors that were significantly associated with pain prolongation.ResultsLogistic regression analysis using a stepwise method revealed that the independent factor significantly associated with failure for 50% pain reduction at the final follow-up visit was VAS reduction rate at week 3 (OR = 9.0, 95% CI 0.5–17.6, p = 0.04).ConclusionsPatients who responded well to treatment reported a reduction in pain during the first 3 weeks of treatment versus nonresponders who did not. It may be possible to predict the prognosis of osteoporotic vertebral fractures early during the first 3 weeks after hospital admission.
Evaluation of therapeutic effects of KC7F2, temozolomide, and its combination on genes involved in the Wnt signal pathway in the U87MG glioma cell line
Authors: Korkmaz Hakan , Korkmaz Bulent , Bagca Bakiye Goker , Takanlou Leila Sabour , Takanlou Maryam Sabour , shademan behrouz , Abbaszade Zaka , Özateş Neslihan Pınar , Avci Cıgır Biray 
Published: February 2022

Abstract
Glioma is the most common malignant primary brain tumor that survives less than 12 months after diagnosis. The Wnt signaling pathway creates a complex network of proteins that have different effects on cells, so they can be involved in many cancers, including gliomas. Drug resistance is seen in many cancer treatments. Therefore, we investigated the combined effect of Temozolomide (TMZ) and KC7F2 on cell survival and changes in gene expression in the Wnt signal pathway. The effects of TMZ and KC7F2 on cancer cell viability and proliferation were investigated by WST-1 method. Isobologram analysis was performed to evaluate the combined effects of TMZ and KC7F on U87MG glioma cell line. Also, Quantitative RT-PCR was used to evaluate the mRNA expression level of genes related to the Wnt signal pathway in treated cancer cells and control groups. IC50 TMZ and KC7F2 values in U87MG cell lines were determined to be 461µM and 19µM in 48 hours, respectively. Combined effects of TMZ and KC7F2 on the U87MG cell line show the CI values for TMZ and KC7F2 were found as 0.746. It was found that KC7F2+TMZ increased APC, CCND1, CXXC4, DAAM1, FBXW11, FRZB, FZD3, FZD8, LEF1, LRP5, LRP6, PYGO1, RHOA, RHOU, RUVBL1, VANGL2, WIF1, WNT10A, WNT16, WNT3A, and WNT6 while decreasing NKD1, PRICKLE1, DKK1, WNT1 expressions in U87MG cell lines. The results show that the combination of TMZ and KC7F2 can be a promising anti-cancer agent for the treatment of glioblastoma through the Wnt / β-catenin signaling pathway. However, further studies are needed to understand all angles.
Antibody-Dependent Enhancement of IL-6 Production by SARS-CoV-2 Nucleocapsid Protein
Authors: Nakayama Emi E. , Kubota-Koketsu Ritsuko , Sasaki Tadahiro , Suzuki Keita , Uno Kazuko , Shimizu Jun , Okamoto Toru , Matsumoto Hisatake , Matsuura Hiroshi , Hashimoto Shoji , Tanaka Toshio , Harada Hiromasa , Tomita Masafumi , Kaneko Mitsunori , Yoshizaki Kazuyuki , Shioda Tatsuo 
Published: February 2022

Abstract
A cytokine storm induces acute respiratory distress syndrome, the main cause of death in coronavirus disease 2019 (COVID-19) patients. However, the detailed mechanisms of cytokine induction due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain unclear. To examine the cytokine production in COVID-19, we mimicked the disease in SARS-CoV-2-infected alveoli by adding the lysate of SARS-CoV-2-infected cells to cultured macrophages or induced pluripotent stem cell-derived myeloid cells. The cells secreted interleukin (IL)-6 after the addition of SARS-CoV-2-infected cell lysate. Screening of 25 SARS-CoV-2 protein-expressing plasmids revealed that the N protein-coding plasmid alone induced IL-6 production. The addition of anti-N antibody further enhanced IL-6 production, but the F(ab’)2 fragment did not. Sera from COVID-19 patients also enhanced IL-6 production, and sera from patients with severer disease induced higher levels of IL-6. These results suggest that anti-N antibody promotes IL-6 production in SARS-CoV-2-infected alveoli, leading to the cytokine storm of COVID-19. (150 words)
Torsional And Lateral Eigenmode Oscillations For Atomic Resolution Imaging Of HOPG In Air Under Ambient Conditions
Authors: Eichhorn Anna L. , Dietz Christian 
Published: February 2022

Abstract
Combined in-plane and out-of-plane multifrequency atomic force microscopy techniques have been demonstrated to be important tools to decipher spatial differences of sample surfaces at the atomic scale. The analysis of physical properties perpendicular to the sample surface is routinely achieved from flexural cantilever oscillations, whereas the interpretation of in-plane sample properties via force microscopy is still challenging. Besides the torsional oscillation, there is the additional option to exploit the lateral oscillation of the cantilever for in-plane surface analysis. In this study, we used different multifrequency force microscopy approaches to attain better understanding of the interactions between a super-sharp tip and an HOPG surface focusing on the discrimination between friction and shear forces. We found that the lateral eigenmode is suitable for the determination of the shear modulus whereas the torsional eigenmode provides information on local friction forces between tip and sample. Based on the results, we propose that the full set of elastic constants of graphite can be determined from combined in-plane and out-of-plane multifrequency atomic force microscopy if ultrasmall amplitudes and high force constants are used.
A Simple Pooling Method for Variable Selection in Multiply Imputed Datasets Outperformed Complex Methods
Authors: Heymans MW , Panken AM 
Published: February 2022

Abstract
Background. For the development of prognostic models, after multiple imputation, variable selection is advised to be applied from the pooled model. The aim of this study is to evaluate by using a simulation study and practical data example the performance of four different pooling methods for variable selection in multiple imputed datasets. These methods are the D1, D2, D3 and recently extended Median-P-Rule (MPR) for categorical, dichotomous, and continuous variables in logistic regression models. Methods. Four datasets (n=200 and n=500), with 9 variables and correlations of respectively 0.2 and 0.6 between these variables, were simulated. These datasets included 2 categorical and 2 continuous variables with 20% missing at random data. Multiple Imputation (m=5) was applied, and the four methods were compared with selection from the full model (without missing data). The same analyzes were repeated in five multiply imputed real-world datasets (NHANES) (m=5, p=0.05, N=250/300/400/500/1000). Results. In the simulated datasets, the differences between the pooling methods were most evident in the smaller datasets. The MPR performed equal to all other pooling methods for the selection frequency, as well as for the P-values of the continuous and dichotomous variables, however the MPR performed consistently better for pooling and selecting categorical variables in multiply imputed datasets and also regarding the stability of the selected prognostic models. Analyzes in the NHANES-dataset showed that all methods mostly selected the same models. Compared to each other however, the D2-method seemed to be the least sensitive and the MPR the most sensitive, most simple, and easy method to apply. Conclusions. Considering that MPR is the most simple and easy pooling method to use for epidemiologists and applied researchers, we carefully recommend using the MPR-method to pool categorical variables with more than two levels after Multiple Imputation in combination with Backward Selection-procedures (BWS). Because MPR never performed worse than the other methods in continuous and dichotomous variables we also advice to use MPR in these types of variables.