bluuebunny/crossref_metadata_2025_split huggingface dataset:
Latent class analysis of imaging and clinical respiratory parameters from patients with COVID-19-related ARDS identifies recruitment subphenotypes
Authors: Filippini Daan F. L. , Di Gennaro Elisa , van Amstel Rombout B. E. , Beenen Ludo F. M. , Grasso Salvatore , Pisani Luigi , Bos Lieuwe D. J. , Smit Marry R. 
Published: November 2022

Abstract
Background
Patients with COVID-19-related acute respiratory distress syndrome (ARDS) require respiratory support with invasive mechanical ventilation and show varying responses to recruitment manoeuvres. In patients with ARDS not related to COVID-19, two pulmonary subphenotypes that differed in recruitability were identified using latent class analysis (LCA) of imaging and clinical respiratory parameters. We aimed to evaluate if similar subphenotypes are present in patients with COVID-19-related ARDS.

Methods
This is the retrospective analysis of mechanically ventilated patients with COVID-19-related ARDS who underwent CT scans at positive end-expiratory pressure of 10 cmH2O and after a recruitment manoeuvre at 20 cmH2O. LCA was applied to quantitative CT-derived parameters, clinical respiratory parameters, blood gas analysis and routine laboratory values before recruitment to identify subphenotypes.

Results
99 patients were included. Using 12 variables, a two-class LCA model was identified as best fitting. Subphenotype 2 (recruitable) was characterized by a lower PaO2/FiO2, lower normally aerated lung volume and lower compliance as opposed to a higher non-aerated lung mass and higher mechanical power when compared to subphenotype 1 (non-recruitable). Patients with subphenotype 2 had more decrease in non-aerated lung mass in response to a standardized recruitment manoeuvre (p = 0.024) and were mechanically ventilated longer until successful extubation (adjusted SHR 0.46, 95% CI 0.23–0.91, p = 0.026), while no difference in survival was found (p = 0.814).

Conclusions
A recruitable and non-recruitable subphenotype were identified in patients with COVID-19-related ARDS. These findings are in line with previous studies in non-COVID-19-related ARDS and suggest that a combination of imaging and clinical respiratory parameters could facilitate the identification of recruitable lungs before the manoeuvre.
CXCL1 promotes colon cancer progression through activation of NF-κB/P300 signaling pathway
Authors: Zhuo Changhua , Ruan Qiang , Zhao Xiangqian , Shen Yangkun , Lin Ruirong 
Published: November 2022

Abstract
Background
The upregulated expression of CXCL1 has been validated in colorectal cancer patients. As a potential biotherapeutic target for colorectal cancer, the mechanism by which CXCL1 affects the development of colorectal cancer is not clear.

Methods
Expression data of CXCL1 in colorectal cancer were obtained from the GEO database and verified using the GEPIA database and the TIMER 2.0 database. Knockout and overexpression of CXCL1 in colorectal cancer cells by CRISPR/Cas and "Sleeping Beauty" transposon-mediated gene editing techniques. Cell biological function was demonstrated by CCK-8, transwell chamber and Colony formation assay. RT-qPCR and Western Blot assays measured RNA and protein expression. Protein localization and expression were measured by immunohistochemistry and immunofluorescence.

Results
Bioinformatics analysis showed significant overexpression of CXCL1 in the colorectal cancer tissues compared to normal human tissues, and identified CXCL1 as a potential therapeutic target for colorectal cancer. We demonstrate that CXCL1 promotes the proliferation and migration of colon cancer cells and has a facilitative effect on tumor angiogenesis. Furthermore, CXCL1 elevation promoted the migration of M2-tumor associated macrophages (TAMs) while disrupting the aggregation of CD4+ and CD8+ T cells at tumor sites. Mechanistic studies suggested that CXCL1 activates the NF-κB pathway. In the in vivo colon cancer transplantation tumor model, treatment with the P300 inhibitor C646 significantly inhibited the growth of CXCL1-overexpressing colon cancer.

Conclusion
CXCL1 promotes colon cancer development through activation of NF-κB/P300, and that CXCL1-based therapy is a potential novel strategy to prevent colon cancer development.
A novel design for biodiesel production from methanol + mutton bone fat mixture
Authors: Farokhnia Ali , Jokar Seyyed Mohammad , Parvasi Payam , Kim Albert S. 
Published: November 2022

AbstractBioenergy plays a significant role in the green transition. In this work, the conversion of methanol and mutton bone fat oil (as a low-cost feedstock) for bioenergy production was studied. The five-level, three-factor response surface methodology (RSM) was used to optimize the transesterification reaction conditions for produced biodiesel. Twenty ultrasonic-assisted experiments at the frequency of 25 kHz were conducted to investigate the effects of methanol/oil molar ratio (M/O) and concentrations of KOH and NaOH as catalysts on biodiesel yield. A second-order polynomial equation was developed by fitting the RSM experimental data using Design-Expert software. Results showed that the optimum biodiesel yield of 90.087% could be achieved by the KOH catalyst with 2.5 wt% concentration and 15:1 M/O during 3 h of the reaction. Furthermore, the biofuel analyses showed that methanol and mutton bone fat oil can be used as a proper feedstock for biofuel production. In the following, a membrane filtration package system is proposed and modeled. The reaction kinetics was determined based on experimental data. The results of the mathematical modeling showed the reaction time appears to be 6 times shorter in a membrane setup (30 min). Consequently, membrane application is highly recommended for biodiesel production from mutton bone fat oil.
An algorithm for solving the Navier–Stokes problem with mixed boundary conditions
Authors: Abdelwahed Mohamed , Chorfi Nejmeddine , Mezghani Najeh , Ouertani Henda 
Published: November 2022

AbstractWe consider a time-dependent Navier–Stokes problem in dimension two and three provided with mixed boundary conditions. We propose an iterative algorithm and its implementation for resolving this considered problem. The discretization is based on a backward Euler scheme with respect to the time variable and the spectral method with respect to the space variables. We present some numerical experiments which confirm the interest of the discretization.
Antimicrobial resistance and rational use of medicine: knowledge, perceptions, and training of clinical health professions students in Uganda
Authors: Kanyike Andrew Marvin , Olum Ronald , Kajjimu Jonathan , Owembabazi Shebah , Ojilong Daniel , Nassozi Dianah Rhoda , Amongin Joan Fidelia , Atulinda Linda , Agaba Kenneth , Agira Drake , Wamala Nicholas Kisaakye , Buule Richard , Nabukeera Germinah , Kyomuhendo Robert , Luwano Rehema , Owobusingye Whitney , Matovu Dissan , Musoke Philip , Bongomin Felix , Kiyimba Kenedy 
Published: November 2022

Abstract
Background
Antimicrobial resistance (AMR) is an important global health concern, projected to contribute to significant mortality, particularly in developing countries. This study aimed to determine the knowledge, perceptions of clinical health professions students towards antimicrobial resistance and rational use of medicine and confidence level to prescribe antimicrobials.

Methods
An online descriptive cross-sectional survey was conducted among clinical health professions students across 9 medical schools in Uganda. A semi-structured questionnaire using Kobo Toolbox form was shared among participants via WhatsApp Messenger (Meta, California, USA). Knowledge was categorized using modified Bloom’s cut-off. One-way ANOVA, Chi-square or Fisher’s exact test, and logistic regression were used to assess the association between dependent and independent variables. A p < 0.05 was considered statistically significant.

Results
We surveyed 681 participants, most were pursuing a Bachelor of Medicine and Surgery degree (n = 433, 63.6%), with a mean age of 24 (standard deviation: 3.6) years. Most participants (n = 596, 87.5%) had sufficient knowledge about antimicrobial resistance with a mean score of 85 ± 14.2%. There was a significant difference in mean knowledge scores of year 4 (86.6%) compared to year 3 (82.4%) (p = 0.002) and year 5 (88.0%) compared to year 3 (82.4%) (p < 0.001). Most participants (n = 456, 66.9%), were confident on making an accurate diagnosis of infection, and choosing the correct antimicrobial agent to use (n = 484, 71.1%).

Conclusion
Health profession students exhibited good knowledge on antimicrobial resistance and high self-perceived confidence that should be leveraged to foster better future antimicrobial prescription practices. However, they still agreed that a separated course unit on AMR is necessary which responsible authorities should consider to consolidate the efforts.
Social media analysis of Twitter tweets related to ASD in 2019–2020, with particular attention to COVID-19: topic modelling and sentiment analysis
Authors: Corti Luca , Zanetti Michele , Tricella Giovanni , Bonati Maurizio 
Published: November 2022

Abstract
Background
Social media contains an overabundance of health information relating to people living with different type of diseases. Autism spectrum disorder (ASD) is a complex neurodevelopmental condition with lifelong impacts and reported trends have revealed a considerable increase in prevalence and incidence. Research had shown that the ASD community provides significant support to its members through Twitter, providing information about their values and perceptions through their use of words and emotional stance. Our purpose was to analyze all the messages posted on Twitter platform regarding ASD and analyze the topics covered within the tweets, to understand the attitude of the various people interested in the topic. In particular, we focused on the discussion of ASD and COVID-19.

Methods
The data collection process was based on the search for tweets through hashtags and keywords. After bots screening, the NMF (Non-Negative Matrix Factorization) method was used for topic modeling because it produces more coherent topics compared to other solutions. Sentiment scores were calculated using AFiNN for each tweet to represent its negative to positive emotion.

Results
From the 2.458.929 tweets produced in 2020, 691.582 users were extracted (188 bots which generated 59.104 tweets), while from the 2.393.236 total tweets from 2019, the number of identified users was 684.032 (230 bots which generated 50.057 tweets). The total number of COVID-ASD tweets is only a small part of the total dataset. Often, the negative sentiment identified in the sentiment analysis referred to anger towards COVID-19 and its management, while the positive sentiment reflected the necessity to provide constant support to people with ASD.

Conclusions
Social media contributes to a great discussion on topics related to autism, especially with regards to focus on family, community, and therapies. The COVID-19 pandemic increased the use of social media, especially during the lockdown period. It is important to help develop and distribute appropriate, evidence-based ASD-related information.
Evaluation the effects of red yeast rice in combination with statin on lipid profile and inflammatory indices; a randomized clinical trial
Authors: Tavan Ali , Noroozi Saam , Zamiri Bardia , Gholchin Vafa Reza , Rahmani Mohammadhossein , Mehdizadeh Parizi Mohammadjavad , Ahmadi Amin , Heydarzade Reza , Montaseri Mohammad , Hosseini Seyed Ali , Kojuri Javad 
Published: November 2022

Abstract
Background
Dyslipidemia is a prominent cause of cardiovascular disease as it leads to inflammation and plaque deposition within arteries. Treatment includes lifestyle modifications and lipid-lowering medications. We aimed to assess the therapeutic effects of red yeast rice (RYR) alongside statin therapy.

Methods
This triple-blind randomized clinical trial involved 92 dyslipidemia patients and was performed in 2019. Standard laboratory tests were used to assess the serum LDL cholesterol (LDL-C), HDL cholesterol (HDL-C), total cholesterol, triglyceride (TG), and high sensitivity C-reactive protein (hs-CRP) levels. Subsequently, patients randomly received one daily RYR or placebo tablet for 1 month beside routine single statin therapy. Subsequently, blood tests were repeated and compared against the baseline. Liver function tests were also requested.

Results
Total cholesterol significantly (P = 0.019) decreased in the treatment group (− 10.2 mg/dL) compared with the placebo group (− 1.3 mg/dL). HDL cholesterol decreased by 2.19 mg/dL in the treatment group but increased by 0.53 mg/dL in the treatment group (P = 0.083). LDL cholesterol declined in both placebo (− 5.09) and treatment (− 0.73) groups (P = 0.187). TG increased by about 7 mg/dL in the treatment group but fell by roughly 1 mg/dL in the placebo group (P = 0.386). Hs-CRP increased by 0.28 mg/dL in the treatment group but decreased by 0.09 mg/dL in the placebo group (P = 0.336).

Conclusions
We found that adding RYR (Lesstat®) to statin medications significantly decreases total cholesterol. However, no significant effect was seen on other lipid profile components or Hs-CRP. Finally, we showed that RYR is safe to add to statins considering liver function (clinicaltrials.gov: NCT05095480).
Effectiveness of home-based “egg-suji” diet in management of severe acute malnutrition of Rohingya refugee children
Authors: Roy S. K. , Jahan Khurshid , Khatoon Soofia , Alam Nurul , Tasnim Saria , Parveen Shahana , Ferdaus Ambrina , Cubra Khadijatul 
Published: November 2022

Abstract
Background
Prevalence of severe acute malnutrition (SAM) among Rohingya children aged 6–59 months who took shelter in refugee camp in Cox’s Bazar District, Bangladesh, was found to be 7.5%.

Objective
To measure the effectiveness of homemade diet in the management of severe acute malnutrition of Rohingya refugee children.

Methods
In total, 645 SAM children (MUAC < 11.5 cm) aged 6–59 months were selected and fed the homemade diet for 3 months by their caregivers and followed up for next 2 months. Nutrition counseling, demonstration of food preparation and the ingredients of food (rice powder, egg, sugar and oil) were provided to the families for 3 months to cook “egg-suji” diet to feed the children.

Results
The study children were assessed for nutritional status. After intervention, energy intake from diet increased from 455.29 ± 120.9 kcal/day to 609.61 ± 29.5 kcal/day (P = 0.001) in 3 months. Frequency of daily food intake improved from 4.89 ± 1.02 to 5.94 ± 0.26 (P = 0.001). The body weight of children increased from 6.3 ± 1.04 kg to 9.93 ± 1.35 kg (P = 0.001), height increased from 67.93 ± 6.18 cm to 73.86 ± 0.35 (P = 0.001) cm, and MUAC improved from 11.14 ± 1.35 cm to 12.89 ± 0.37 cm (P = 0.001). HAZ improved from − 3.64 ± 1.35 to − 2.82 ± 1.40 (P = 0.001), WHZ improved from − 2.45 ± 1.23 to 1.03 ± 1.17 (P = 0.001), WAZ improved from − 3.8 ± 0.61 to − 0.69 ± 0.78, and MUACZ improved from − 3.32 ± 0.49 to 1.8 ± 0.54 (P = 0.001) from the beginning to the end of observation. Morbidity was found in 5.12% children in the first month which reduced to 0.15% at the end of follow-up.

Conclusions
Nutritional counseling and supply of food ingredients at refugee camps resulted in complete recovery from severe malnutrition for all children which was sustainable.
Increased Meal Size but Reduced Meal-Stimulated Plasma Cholecystokinin Concentrations in Women With Obesity
Authors: Geary Nori , Asarian Lori , Graf Gwendolyn , Gobbi Susanna , Tobler Philippe N , Rehfeld Jens F , Leeners Brigitte 
Published: November 2022

AbstractTo better understand the physiological basis of obesity in women, we investigated whether obesity or menstrual cycle phase affects laboratory test-meal size or meal-stimulated plasma cholecystokinin (CCK) concentration. Women with healthy weight (body mass index [BMI] of 18.5-24.9 kg/m2, N = 16) or obesity (BMI 30-39.9 kg/m2, N = 20) were tested once in the late-follicular or peri-ovulatory phase (LF/PO) and once in the mid-luteal phase (ML). Meals of ham sandwiches were offered and blood was sampled. Menstrual cycle phases were verified with participants’ reports of menses and measurements of progesterone and luteinizing hormone (LH) concentrations. Women with obesity ate significantly larger meals than women with healthy weight, (mean, 711 [95% CI, 402-1013] kJ, P = 0.001, during the LF/PO and 426 [105-734] kJ, P = 0.027, larger during the ML). Women with healthy weight ate smaller meals during LF/PO than ML (decrease, 510 [192-821 kJ], P = 0.008), but women with obesity did not (decrease, 226 [−87-542] kJ, P = 0.15). CCK concentrations 18 to 30 minutes after meal onset were lower in women with obesity than in women with healthy weight during LF/PO (3.6 [3.1-4.1] vs 6.1 [4.5-7.7] pmol/L; P = 0.004), but not during ML, with a significant interaction effect (1.8 [1.2-2.4] pmol/L, P = 0.048). Women with obesity consumed larger meals than women with healthy weight but displayed reduced meal-stimulated plasma CCK concentrations. These data are consistent with the hypothesis that a defect in CCK secretion compromises satiation in obese women and contributes to the development or maintenance of obesity.
POU1F1/Pou1f1 c.143-83A &gt; G Variant Disrupts the Branch Site in Pre-mRNA and Leads to Dwarfism
Authors: Akiba Kazuhisa , Hasegawa Yukihiro , Katoh-Fukui Yuko , Terao Miho , Takada Shuji , Hasegawa Tomonobu , Fukami Maki , Narumi Satoshi 
Published: November 2022

Abstract
POU Class 1 Homeobox1 (POU1F1/Pou1f1) is a well-established pituitary-specific transcription factor, and causes, when mutated, combined pituitary hormone deficiency in humans and mice. POU1F1/Pou1f1 has 2 isoforms: the alpha and beta isoforms. Recently, pathogenic variants in the unique coding region of the beta isoform (beta domain) and the intron near the exon–intron boundary for the beta domain were reported, although their functional consequences remain obscure. In this study, we generated mice carrying the Pou1f1 c.143-83A&gt;G substitution that recapitulates the human intronic variant near the exon–intron boundary for the beta domain. Homozygous mice showed postnatal growth failure, with an average body weight that was 35% of wild-type littermates at 12 weeks, which was accompanied by anterior pituitary hypoplasia and deficiency of circulating insulin-like growth factor 1 and thyroxine. The results of RNA-seq analysis of the pituitary gland were consistent with reduction of somatotrophs, and this was confirmed immunohistochemically. Reverse transcription polymerase chain reaction of pituitary Pou1f1 mRNA showed abnormal splicing in homozygous mice, with a decrease in the alpha isoform, an increase in the beta isoform, and the emergence of the exon-skipped transcript. We further characterized artificial variants in or near the beta domain, which were candidate positions of the branch site in pre-mRNA, using cultured cell–basis analysis and found that only c.143-83A&gt;G produced transcripts similar to the mice model. Our report is the first to show that the c.143-83A&gt;G variant leads to splicing disruption and causes morphological and functional abnormalities in the pituitary gland. Furthermore, our mice will contribute understanding the role of POU1F1/Pou1f1 transcripts in pituitary development.
Piglet cardiopulmonary bypass induces intestinal dysbiosis and barrier dysfunction associated with systemic inflammation
Authors: Salomon Jeffrey D. , Qiu Haowen , Feng Dan , Owens Jacob , Khailova Ludmila , Osorio Lujan Suzanne , Iguidbashian John , Chhonker Yashpal S. , Murry Daryl J. , Riethoven Jean-Jack , Lindsey Merry L. , Singh Amar B. , Davidson Jesse A. 
Published: November 2022

ABSTRACTThe intestinal microbiome is essential to human health and homeostasis, and is implicated in the pathophysiology of disease, including congenital heart disease and cardiac surgery. Improving the microbiome and reducing inflammatory metabolites may reduce systemic inflammation following cardiac surgery with cardiopulmonary bypass (CPB) to expedite recovery post-operatively. Limited research exists in this area and identifying animal models that can replicate changes in the human intestinal microbiome after CPB is necessary. We used a piglet model of CPB with two groups, CPB (n=5) and a control group with mechanical ventilation (n=7), to evaluate changes to the microbiome, intestinal barrier dysfunction and intestinal metabolites with inflammation after CPB. We identified significant changes to the microbiome, barrier dysfunction, intestinal short-chain fatty acids and eicosanoids, and elevated cytokines in the CPB/deep hypothermic circulatory arrest group compared to the control group at just 4 h after intervention. This piglet model of CPB replicates known human changes to intestinal flora and metabolite profiles, and can be used to evaluate gut interventions aimed at reducing downstream inflammation after cardiac surgery with CPB.
Temperature and Light Multiphysical Fields Modulate the Propagation of Chemical Waves on Multiple Length Scales in a System
Authors: Zhang Baoying 
Published: November 2022

Abstract
The phenomenon of multiple length scales exists in plants during growth, which is influenced not only by light but also by other physical fields such as substance concentration and temperature, and the combined effects of various physical fields form a complex multi-scale phenomenon. However, the mechanism of the multi-physical interaction of temperature and light on the formation of multiple length scales in plants has not been investigated. In this paper, we coupled a gel with a Belousov-Zhabotinsky (BZ) chemical reaction system, constructed a one-dimensional open reaction diffusion system, and investigated the changes in the multiple length scale periodic structure of chemical waves in the one-dimensional gel system under the combined effect of multiple physical fields of light and temperature. It was found that when increasing the light intensity at T = 26 °C or decreasing the temperature at the light intensity I ≤ 50 µW·cm−2, the chemical wave multiple length scale period structure of the system changed as follows: P8 → P16 → P8 → P4 → P2 → P1, and the chemical wave period structure complexity showed a non-linear change of first increasing and then decreasing. When increasing the light intensity at T ≤ 24 °C or decreasing the temperature at the light intensity I &gt; 50 µW·cm−2, the chemical wave period structure complexity of the system shows a linear decrease.