bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipase production from Aniba canelilla endophytic fungi, characterization and application of the enzymatic extract
Authors: Matias Rosiane Rodrigues , Rodrigues Juliana Gisele Corrêa , Procópio Rudi Emerson de Lima , Matte Carla Roberta , Duvoisin Junior Sergio , Ayub Marco Antonio Zachia , Soares Rosane Michele Duarte , Albuquerque Patrícia Melchionna 
Published: September 2022

Endophytic fungi (EF) have a notable capacity to produce active molecules of industrial importance, such as hydrolytic enzymes. In this study we investigated the production of lipase by EFs isolated from the Amazonian species Aniba canelilla (Lauraceae), characterized the enzymatic extract obtained from the most promising fungus, and applied the lipolytic extract as a biocatalyst in the transesterification reaction for biodiesel production. The fungi were submitted to enzymatic screening in solid medium and in submerged fermentation to assess their lipase production. A total of 292 fungi were tested in solid media. Lipolytic activity was detected in 74% of the fungi cultivated in liquid media, 18 of which showing promising enzymatic production. The best lipase producer, Endomelanconiopsis endophytica QAT_7AC, was identified by sequencing of the ITS region. After adjusting the bioprocess conditions, E. endophytica QAT_7AC produced 2,415.5 U/mL of lipase after 72 h. The enzymatic extract showed higher lipolytic activity under pH 8.0 and 40 oC. The extract was applied as a biocatalyst in a transesterification reaction performed at 40 oC, with ethanol and waste cooking oil (3:1). The biodiesel yield was found to be 87% after 2 h rection when the fungal enzyme was used and 89% with the commercial biocatalyst. The endophytic fungi isolated from A. canelilla proved themselves to be biotechnologically relevant, as they can be explored as potential producers of lipases. The lipolytic extract can be applied in the synthesis of biodiesel using waste cooking oil.
Manifestações orais em pacientes portadores da Diabetes mellitus
Authors: Fernandes Jefferson Douglas Lima , Bomfim Rafael Meneses , França Caroline Reis , Carneiro Grace Kelly Martins , Silva Ana Carolina Resende da , Nascimento Neto Edvaldo Vieira do , Santos Müller Gomes dos , Silva Genilson Campos da , Cabral Esther Chrystyane Costa , Santos Ana Lethícia Leão 
Published: September 2022

O termo “Diabetes mellitus” é usado para identificar um grupo de distúrbios caracterizados por níveis elevados de glicose no sangue. Essa elevação é resultado de uma deficiência na secreção de insulina ou de um aumento da resistência celular às ações da insulina, levando a uma variedade de anormalidades metabólicas envolvendo carboidratos, gorduras e proteínas. Diante do exposto, esse estudo objetivou revisar a literatura acerca dos anestésicos locais utilizados na odontologia, destacando informações necessárias quanto à escolha e quantidade de anestésica, prevenindo a ocorrência de complicações. Para a construção deste trabalho foi feito um levantamento bibliográfico nas bases de dados SciVerse Scopus, Scientific Eletronic Library Online (Scielo), U.S. National Library of Medicine (PUBMED) e ScienceDirect, utilizando o gerenciador de referências Mendeley. As principais manifestações orais e complicações relacionadas ao quadro da Diabetes Mellitus envolvem a xerostomia, disgeusia, infecções orais, cárie dentária e doenças periodontais. Também há relatos de lesões na mucosa oral, como estomatite, língua geográfica, glossite migratória benigna, língua fissurada, úlcera traumática, líquen plano, reação liquenóide oral e quelite angular. A prevalência e susceptibilidade de desenvolver essas lesões apresenta-se maiores em pacientes com DM em comparação com aqueles considerados controles. O Diabetes Mellitus é uma doença crônica, não transmissível e endêmica, que resultam em múltiplas complicações, que aumentam a medida quando o controle glicêmico do paciente é inadequado. Assim, médicos e dentistas devem estar atentos às diversas manifestações orais do diabetes para fazer um diagnóstico precoce.
O papel do enfermeiro na auditoria hospitalar sobre o controle de infecções relacionadas à assistência em saúde
Authors: Antunes Pamela Soares Santana de Macedo , Sá Bruna Letícia Carvalho de , Leal Evelin Peixoto Braga Vieira , Oliveira Larissa da Silva Oliveira , Figueiredo Rafaela Muniz Pinto de , Vieira Raphael Pessoa Custodio , Silva Samya Pureza da 
Published: September 2022

Introdução: A auditoria visa o controle de custos, a qualidade do atendimento ao paciente, pagamento justo da conta hospitalar, a transparência da negociação. Na enfermagem, a auditoria é uma atividade dedicada à eficácia de serviços. As infecções relacionadas à assistência em saúde adquiridas durante a prestação dos cuidados de saúde são exemplos de eventos adversos que impacta na qualidade dos serviços prestados. Objetivo: Descrever o papel do enfermeiro na auditoria hospitalar sobre o controle de infecções relacionadas à assistência em saúde. Metodologia: Trata-se de um estudo de abordagem qualitativa, do tipo revisão integrativa, onde será abordado auditoria em saúde sobre o controle de infecções relacionadas à assistência em saúde. Foi realizado por meio de busca em base de dados disponíveis na Biblioteca Virtual de Saúde (BVS) através dos descritores em saúde: enfermeiros, infecções relacionadas à assistência em saúde e auditoria em enfermagem, no idioma português, respeitando o marco temporal de 5 anos (2019-2022). Resultados: Para o desenvolvimento do estudo foram realizadas leituras e análises minuciosas de cinco documentos encontrados a partir da busca realizada previamente. Considerações Finais: Infecções relacionadas à assistência em saúde são apenas um dos muitos aspectos relevantes para a auditoria em saúde, representam baixa qualidade na assistência em saúde. O enfermeiro auditor faz parte de uma necessidade a instituição de saúde fiscalizando como estão sendo realizados os registros, tendo uma visão crítica e propositora, desempenhando suas atribuições que visam à melhoria e qualidade no atendimento e a redução de perdas.
Paracoccidioidomicose em um adulto imunocompetente no Sul de Minas Gerais: Relato de caso
Authors: Raimundo Ana Paula de Araújo , Maia Beatriz Gianini de Oliveira Burza , Marotti Jaqueline Brandão Guerreiro 
Published: September 2022

A Paracoccidioidomicose (PCM), é uma infecção instaurada após a inalação de partes fúngicas, dispersas na atmosfera. Acomete principalmente o sexo masculino e moradores da zona rural, visto que há maior incidência do fungo nesse meio. Apresenta diversas manifestações clínicas, dificultando assim o desfecho do diagnóstico. Nesse relato em questão, é descrito o caso de um senhor de 59 anos com histórico de síncope, seguido de diversas convulsões do tipo tônico-clônica generalizadas. Após internação e com diversos exames complementares, a investigação culminou na análise anatomopatológica da ressecção transretal da próstata, investigando a presença de fungos, na qual resultou positiva pela coloração de PAS e Gragott da Paracoccidioides brasiliensis. O paciente foi submetido ao tratamento com Sulfametoxazol 400 mg +Trimetoprima 80 mg, por dois anos, até que se obteve uma melhora clínica e dos resultados de exames complementares solicitados. O paciente está sob monitoramento e não apresenta recidivas da doença, até o momento.
Sağlık Çalışanları Penceresinden Pandemi Süreci
Authors: ONAN Nevin , DİNÇ Sevgi , DEMİR Zeynep 
Published: September 2022

Amaç: Araştırmada sağlık çalışanlarının pandemi sürecini ve bu süreçte sağlık çalışanı olmayı nasıl algıladıklarının metafor analizi yöntemi ile belirlenmesi amaçlandı.
Gereç ve Yöntemler: Fenomonolojik desenin kullanıldığı nitel tipteki araştırma, Nisan- Mayıs 2021 tarihleri arasında bir araştırma hastanesinde çalışan 264 sağlık çalışanı ile yapıldı. Veriler çevrim içi anket tekniği ile Google formlar aracılığıyla toplandı. Nitel verilerin değerlendirilmesinde içerik analizi ve betimsel analiz tekniği kullanıldı.
Bulgular: Sağlık çalışanlarının pandemiye yönelik 219 metafor oluşturduğu ve bunlardan 104 metaforun frekanslarının n=1 olduğu belirlendi. Pandemiye yönelik olarak deprem, savaş, karanlık, ateş ve fırtına metaforlarının ilk beş sırada yer aldığı belirlendi. Pandemide sağlık çalışanı olmaya ilişkin oluşturulan 99 metafordan 70 tanesinin frekansının n=1 olduğu belirlendi. Pandemide sağlık çalışanı olmaya yönelik asker, köle, melek, yıldız ve aydınlık/güneş/ışık metaforlarının ilk beş sırada yer aldığı belirlendi. Oluşturulan metaforlar değerlendirildiğinde, pandemiyi algılamaya yönelik altı ve pandemide sağlık çalışanı olmanın algılanmasına yönelik beş kategori belirlendi. Oluşturulan metaforların sayısına bakıldığında sağlık çalışanlarının pandemi sürecini çok farklı olarak algıladıkları söylenebilir.
Sonuç: Araştırma bulgularına göre; pandemi sürecinin sağlık çalışanlarını fiziksel, ruhsal, sosyal açılardan çok boyutlu olarak etkilediği ve bu sürecin genel anlamda olumsuz algılandığı söylenebilir. Sağlık çalışanlarının pandemi sürecine ve bu süreçte mesleklerine ilişkin algıları göz önünde bulundurularak sağlık çalışanlarına yönelik psikososyal müdahale programlarının planlanıp uygulanmasının önemli olacağı düşünülmektedir.
High-efficiency solution-processed OLED based on trivalent europium complex by modifying the composition of the multiple-host system
Authors: Li Xiaofang , Yin Jiaxuan , Wang Jingyu , Wu Ruixia , Li Shuaibing , Sun Weidong , Zhou Liang 
Published: September 2022

In this work, di-[4-(N,N-ditolylamino)-phenyl]cyclohexane (TAPC); 4,4′,4″-tri (9-carbazoyl)triphenylamine (TcTa); 9-(4-tert-butylphenyl)-3,6-bis(triphenylsilyl)-9H-carbazole (CzSi); and 1,3,5-tri (m-pyrid-3-yl-phenyl)benzene (TmPyPB) were used to constitute the multiple-host system and fabricate solution-processed organic light-emitting diodes (s-OLEDs) with europium complex Eu(DBM)3Phen (DBM, 1,3-diphenylpropane-1,3-dione; Phen,1,10-phenanthroline) as emitter. In order to determine the optimal composition of the multiple-host system, a series of devices with different light-emitting layers (EMLs) were fabricated and compared. Experimental results revealed that removing TmPyPB out of the multiple-host system greatly reduces the turn-on voltage, whereas the addition of TcTa to the multiple-host system helps facilitate the transfer of holes from TAPC to Eu(DBM)3Phen molecules, thus increasing the recombination probability of carriers on emitter molecules. Finally, high performance solution-processed red OLED (turn-on voltage of 3.8 V) based on the europium complex doped multiple-host system obtained the maximum current efficiency of 2.07 cd A−1, power efficiency of 1.54 lm W−1, external quantum efficiency of 1.2%, and brightness of 945 cd m−2.
Association between parity and markers of inflammation: The multi-ethnic study of atherosclerosis
Authors: Ezeigwe Angelica , Ogunmoroti Oluseye , Minhas Anum S. , Rodriguez Carla P. , Kazzi Brigitte , Fashanu Oluwaseun E. , Osibogun Olatokunbo , Kovell Lara C. , Harrington Colleen M. , Michos Erin D. 
Published: September 2022

IntroductionMultiparity has been associated with increased risk of cardiovascular disease (CVD). Inflammation may be a mechanism linking parity to CVD. We investigated the association between parity and later-life markers of inflammation.MethodsWe studied 3,454 female MESA participants aged 45–84, free of CVD, who had data on parity and inflammatory markers. Parity was categorized as 0 (reference), 1–2, 3–4, or ≥5. Linear regression was used to evaluate the association between parity and natural log-transformed levels of fibrinogen, D-dimer, GlycA, high sensitivity C-reactive protein (hsCRP), and interleukin-6 (IL-6).ResultsMean age was 62 ± 10 years. The proportion of women with nulliparity, 1–2, 3–4, and ≥5 live births were 18, 39, 29, and 14%, respectively. There was no association between parity and fibrinogen. Women with grand multiparity (≥5 live births) had 28, 10, and 18% higher levels of hsCRP, IL-6 and D-dimer, respectively, compared to nulliparous women, after adjustment for demographic factors. After additional adjustment for CVD risk factors, women with 1–2 and 3–4 live births had higher hsCRP and women with 1–2 live births had higher GlycA.ConclusionIn this diverse cohort of middle-to-older aged women, we found that higher parity was associated with some inflammatory markers; however, these associations were largely attenuated after adjustment for CVD risk factors. There was no clear dose-response relationship between parity and these inflammatory markers. Future studies are needed to evaluate how inflammation may influence the link between parity and CVD and whether healthy lifestyle/pharmacotherapies targeting inflammation can reduce CVD risk among multiparous women.Clinical trial registrationThe MESA cohort design is registered at clinicaltrials.gov as follows: https://clinicaltrials.gov/ct2/show/NCT00005487.
Case report: Spontaneous bilateral intraocular lens dislocation in a patient with homocystinuria
Authors: Yao Bangtao , Chen Xujian , Liu Gang , Zhao Xiaogui 
Published: September 2022

BackgroundSpontaneous bilateral intraocular lens dislocation of the vitreous cavity is a rare ocular disorder. This article aims to comprehensively describe bilateral spontaneous intraocular lens dislocation with unilateral lamellar macular hole and retinoschisis in a Chinese woman with homocystinuria.Case presentationA 72-year-old Chinese woman with homocystinuria presented with a painless bilateral blurring of vision. The slit lamp showed the absence of lenses in both eyes. B-ultrasound and orbital computed tomography (CT) demonstrated bilateral posterior dislocation of the crystalline lenses, and spectral-domain optical coherence tomography (SD-OCT) revealed a lamellar macular hole and retinoschisis in the right eye. Biochemical examination demonstrated that the total homocysteine level was moderately elevated.ConclusionThis report is the first to present an extensive and valuable description of bilateral intraocular lens dislocation with unilateral lamellar macular hole and retinoschisis secondary to homocystinuria. We have demonstrated that this case was spontaneous and chronic. CT is an effective diagnostic tool for patients with ectopia lentis. Early diagnosis and suitable management of patients with homocystinuria are essential to prevent these complications.
Geological, geophysical, and geochemical constraints on the time-space evolution of Akan composite caldera, Hokkaido, Japan
Authors: Hasegawa Takeshi , Nakagawa Mitsuhiro , Kamiyama Hiroyuki , Yamamoto Akihiko 
Published: September 2022

Some calderas are geometrically complex that may be related not to a single eruption, magma body, or structure. In order to reveal their forming processes, multidisciplinary methods should be applied. Akan volcano has E-W elongated and irregular-shaped caldera (24 × 13 km), implying a complex mechanism of formation. Akan caldera results from successive explosive eruptions from 1.4 to 0.1 Ma. On the basis of duration of dormancy and petrological features (mainly whole-rock major element compositions) of juvenile materials, these eruptions have been grouped into 17 eruptive groups (Ak1–Ak17), each of which consists of a single or a sequential phase. In order to investigate the processes of caldera formation, we focus on the younger eruptive groups (Ak1–Ak7: 0.8 to 0.2 Ma) that have relatively large magnitudes (&gt;10 km3) and likely control the present caldera shape. We performed component analysis of lithic fragments from pyroclastic fallout deposits, whole-rock trace element analysis of juveniles, and gravitational survey of the caldera. We grouped Ak1–Ak7 into three types, namely, type A (Ak1, Ak2), type B (Ak3–Ak5), and type C (Ak6, Ak7), based on the lithic componentry, most of which are accessary and accidental fragments from vent and conduit areas. The characteristic lithic component in each type is as follows: altered rock in type A, aphyric dacite in type B, and pyroxene andesite in type C. These data suggest that explosive eruptions of each type are derived from distinct sources. The whole-rock composition of juvenile materials of each type also shows distinct trends on Harker diagrams. These three chemical trends are nearly parallel, suggesting that three different magma systems were active. This is consistent with the lithic componentry showing three spatially distinct vent sources. The geological and petrological evidence is supported by a Bouguer anomaly map. Akan caldera is characterized by three circular closed minima, indicative of three depressed segments that correspond to the source regions, each of which possibly discharged the three types of magma. Caldera-forming eruptions of Akan caldera occurred from at least three distinct sources with distinct magma systems. In conclusion, Akan caldera is a composite caldera, and its shape reflects the distribution of multiple source regions. The case study of Akan caldera shows a possible time-space evolutionary pathway for a caldera complex where several smaller calderas are nested.
Clinical, hormonal and pathomorphological markers of somatotroph pituitary neuroendocrine tumors predicting the treatment outcome in acromegaly
Authors: Tomasik Agnieszka , Stelmachowska-Banaś Maria , Maksymowicz Maria , Czajka-Oraniec Izabella , Raczkiewicz Dorota , Zieliński Grzegorz , Kunicki Jacek , Zgliczyński Wojciech 
Published: September 2022

BackgroundTranssphenoidal adenomectomy (TSS) of somatotroph pituitary neuroendocrine tumor (PitNET) is the first-line treatment of acromegaly. Pharmacological treatment is recommended if surgery is contraindicated or did not lead to disease remission. The choice of treatment best fitting each patient should be based on thorough investigation of patients’ characteristics. The current analysis attempts to create a tool for personalized treatment planning.AimThis study aimed to assess whether clinical, biochemical, imaging and pathological characteristics can predict surgical remission and response to first-generation somatostatin receptor ligands (SRLs) and pasireotide-LAR in acromegaly.Patients and methodsA retrospective study of 153 acromegaly patients, treated in the Department of Endocrinology in Bielanski Hospital in Warsaw, Poland was performed. Data on demographics, hormonal and imaging results, pathological evaluation, and treatment outcome was extracted from the Polish Acromegaly Registry collecting information from 11 endocrinology centers in Poland and analyzed.ResultsPatients with surgical remission had lower GH and IGF-1 concentrations at diagnosis (median GH 5.5 µg/L [IQR: 3.1-16.0]vs. 19.9 µg/L [IQR: 9.8-42.4], p=&lt;0.001 and mean IGF-1 3.1xULN ± SD=1.2 vs. 3.7xULN ± SD=1.2, p=0.007, respectively) and smaller tumors (median 12.5mm [IQR: 9-19] vs. 23mm [IQR: 18-30], p&lt;0.001). These tumors were more often densely granulated (DG) (73.2% vs. 40.0%, p=0.001) with positive staining for alpha-subunit (α-SU) (58.3% vs. 35.5%, p=0.021) and lower Ki-67 index (p=0.002). Patients responding well to SRLs were more often male (55.6% vs 44.4%, p=0.026), presented lower GH concentration (median GH 17.2 µg/L [IQR: 6.2-29.0] vs. 23.8 µg/L [IQR: 11.2-49.5], p=0.048) and had more often DG tumors (63.0% vs. 14.3%, p&lt;0.001). No significant differences between good and poor-response to pasireotide-LAR groups were found. In multivariate logistic regression analysis fasting GH concentration &lt;8.63 µg/L, maximal tumor diameter &lt;15.5mm, normoprolactinemia and DG tumor turned out to be independent predictors of surgical remission (OR=0.92, p=0.026; OR=0.87, p=0.069, OR=3.86, p=0.096 and OR=3.05, p=0.181, respectively). Fasting GH concentration &lt;36.6 µg/L and DG tumor turned out to be independent predictors of good response to first-generation SRLs (OR=0.96, p=0.06 and OR=10.68, p=0.002, respectively).ConclusionsYounger age at diagnosis, male sex, lower GH, IGF-1 and PRL concentrations, smaller tumor size at diagnosis as well as positive α-SU staining, lower Ki-67 index and DG tumors predicted better treatment outcome in acromegaly patients.
Reactogenicity and immunogenicity of the second COVID-19 vaccination in patients with inborn errors of immunity or mannan-binding lectin deficiency
Authors: Göschl Lisa , Mrak Daniel , Grabmeier-Pfistershammer Katharina , Stiasny Karin , Haslacher Helmuth , Schneider Lisa , Deimel Thomas , Kartnig Felix , Tobudic Selma , Aletaha Daniel , Burgmann Heinz , Bonelli Michael , Pickl Winfried F. , Förster-Waldl Elisabeth , Scheinecker Clemens , Vossen Matthias Gerhard 
Published: September 2022

BackgroundPatients with inborn errors of immunity (IEI) are at increased risk for severe courses of SARS-CoV-2 infection. COVID-19 vaccination provides effective protection in healthy individuals. However, it remains unclear whether vaccination is efficient and safe in patients with constitutional dysfunctions of the immune system. Thus, we analyzed the humoral response, adverse reactions and assessed the disease activity of the underlying disease after COVID-19 vaccination in a cohort of patients suffering from IEIs or mannan-binding lectin deficiency (MBLdef).MethodsVaccination response was assessed after basic immunization using the Elecsys anti-SARS-CoV-2 S immunoassay and via Vero E6 cell based assay to detect neutralization capabilities. Phenotyping of lymphocytes was performed by flow cytometry. Patient charts were reviewed for disease activity, autoimmune phenomena as well as immunization status and reactogenicity of the vaccination. Activity of the underlying disease was assessed using a patient global numeric rating scale (NRS).ResultsOur cohort included 11 individuals with common variable immunodeficiency (CVID), one patient with warts hypogammaglobulinemia immunodeficiency myelokathexis (WHIM) syndrome, two patients with X-linked agammaglobulinemia (XLA), one patient with Muckle Wells syndrome, two patients with cryopyrin-associated periodic syndrome, one patient with Interferon-gamma (IFN-gamma) receptor defect, one patient with selective deficiency in pneumococcal antibody response combined with a low MBL level and seven patients with severe MBL deficiency. COVID-19 vaccination was generally well tolerated with little to no triggering of autoimmune phenomena. 20 out of 26 patients developed an adequate humoral vaccine response. 9 out of 11 patients developed a T cell response comparable to healthy control subjects. Tested immunoglobulin replacement therapy (IgRT) preparations contained Anti-SARS-CoV-2 S antibodies implicating additional protection through IgRT.SummaryIn summary the data support the efficacy and safety of a COVID-19 vaccination in patients with IEIs/MBLdef. We recommend evaluation of the humoral immune response and testing for virus neutralization after vaccination in this cohort.