bluuebunny/crossref_metadata_2025_split huggingface dataset:
FAM83A: A Prognostic Biomarker and Its Correlation with Immune Infiltration in Lung Adenocarcinoma
Authors: Sun Xue , Bai Li-Hong , Xu Pei-Hang , Kuang Yu-Kun , Zheng Yi-Fan , Wang Ji-Yu , Li Zi-Mu , Gan Run-Jing , Tang Ke-Jing 
Published: April 2022

Abstract
Background: FAM83A (Family with sequence similarity 83, member A) has been found to promote tumor cell proliferation and accelerate the progression of several cancer types. However, integrated studies on the prognostic value of FAM83A and its function as an immunotherapy target in pan-cancers remain limited.Methods: Multiple online databases, including ONCOMINE, PrognoScan, Kaplan-Meier plotter, GEPIA, Lung Cancer Explorer, and TIMER, were accessed to evaluate the clinical prognostic value of FAM83A and its association with tumor-infiltrating immune cells across different cancer types.Results: Compared with normal tissues, the expression of FAM83A was higher in breast, colorectal, gastric, head and neck, and lung cancers. High FAM83A expression significantly correlated with inferior overall survival (OS) in lung cancer including lung adenocarcinoma (LUAD). In patients with LUAD, overexpressed FAM83A was negatively associated with infiltration levels of B cells and dendritic cells. In addition, the FAM83A expression level was positively associated with PD-L1, STAT1, STAT3, and JAK1 in LUAD.Conclusions: The high expression level of FAM83A was significantly correlated with a worse prognosis, which was also associated with a decreased level of infiltration of B cells and dendritic cells. FAM83A might increase the expression of PD-L1 through JAK / STAT pathway in the tumor microenvironment of lung adenocarcinoma.
A comparative assessment of the novel water pollution index and the water quality index using empirical data: Improving water quality evaluation
Authors: Amuah Ebenezer Ebo Yahans , Kpiebaya Prosper 
Published: April 2022

Abstract
Different water assessment indices have been employed in the evaluation of water quality for human use. However, these methods have their peculiar drawbacks. This study applied the novel water pollution index (WPI) against the water quality index using empirical data (packaged and groundwater sources) from some parts of Ghana. The study showed that the WPI method is flexible and easy to compute compared to WQI. In this study area, WPI ranged from 0.22–0.31 and 0.23–0.32 for packaged water obtained from production and vending sites respectively. However, the WQI outputs were 0.12–0.36 (production site) and 0.27–0.42 (vending sites). Though the water samples from both sources were excellent for drinking, the wide variation in the WQI suggests the biases resulting from the selection of the weighting factors for the various parameters. Considering the groundwater sources applied in this study, 74% as against 93% and 19% as against 5% of the samples were excellent and good water sources based on the WPI and WQI respectively. These disparities are largely attributed to the biases posed by the quality rating and weighting factors which are researcher-described.
In silico screening of minocycline as an Mpro inhibitor and the adjunctive therapy value for the treatment of COVID-19
Authors: Xiao Bin , Wu Si , Han Yaru , Ren Liwen , Wang Jinhua , Luan Xiurong , Du Guanhua , She Zhanfei 
Published: April 2022

Abstract
To explore potential inhibitors of Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) for the treatment of novel coronavirus disease (COVID-19), in silico screening of 135 clinical drugs was performed targeting on 3-chymotrypsin-like protease (3CLpro, or Mpro). Six drugs including anti-HIV drug (raltegravir), antibacterial drugs (cefonicid, cefoperazone, minocycline), and antidiabetic drugs (canaglifozin, glyburide) showed high binding affinities (≤ -8.5 kcal/mol) and interesting binding conformations compared with the designed co-crystal ligand N3 (-7.7 kcal/mol). In which the antibiotic minocycline, an inhibitor of bacterial ribosomal rRNA, showed the highest binding affinity (-9.6 kcal/mol). Valuable hydrogen bonding and hydrophobic interactions were found between minocycline and Mpro active site. Beside the hydrogen bond with Cys145, minocycline formed a Pi-Cation with His41, which strongly supported minocycline as a Michael Addition acceptor to bind with the catalytic site of Mpro. The structure-affinity relationship was studied based on molecular docking of minocycline analogues. Minocycline showed in vitro Mpro inhibitory activity with IC50 of 5 mM. More importantly, the literature review found that minocycline had both in vitro and in vivo broad-spectrum antiviral as well as anti-inflammatory activities. Minocycline deserves in vivo evaluations as well as randomized controlled trial to investigate the efficacy for the treatment of COVID-19.
The effects of Bone marrow mesenchymal stem cells on m6A RNA methylation modification profile of aged granulosa cells
Authors: Tian Chuan , An Yuanyuan , Zhu Xiangqing , Wei Wei , Ruan Guangping , Zhao Jing , Li Ye , pan xinghua 
Published: April 2022

Abstract
Background
Ovarian ageing causing endocrine disturbances and degenerates systemic tissue and organ functions, seriously affects women's physical and mental health, effective treatment methods are urgently needed. On the basis of our previous studies on juvenile rhesus monkey bone marrow mesenchymal stem cells(BMMSCs) treated ovarian ageing of rhesus monkey, found that BMMSCs improved the ovarian structure and function. This study continues to explore the mechanism of BMMSCs reversd granulosa cell(GC) ageing.
Methods
Established GC ageing model and coculture system of BMMSCs, detected m6A methylation modification changes, performed m6A modified RNA immunoprecipitation sequencing (MeRIP-seq), corrected between m6A peaks with mRNA expression, and identified the expression of hub genes through q-PCR, Immunofluorescence staining, and western blot .
Results
Our results showed that H2O2 induced GC aging successfully and that BMMSCs reversed measures of GC ageing, BMMSCs promoted the expression of FTO and reduced the overall methylation level of m6A. We identified 797 m6A peaks (348 hypomethylated, 449 hypermethylated) and 817 differentially expressed genes (DEGs) (412 upregulated, 405 downregulated) after aged GCs cocultured with BMMSCs, which associated with ovarian function and epigenetic modification. Lysine Demethylase 8 (KDM8) involve in the negative regulation of osteoblast differentiation which was contracted with the positive regulation of osteoblast differentiation to mediate cellular senescence, and downregulated after treated with BMMSCs.
Conclusions
KDM8 may become new target in BMMSCs reversed ovarian ageing.
Catabolic reprogramming of Brassica rapa leaf mesophyll protoplasts during the isolation procedure.
Authors: Watanabe Masami , Nakayama Yoshihiro , Kusano Miyako , Manabe Riichiro 
Published: April 2022

Abstract
In this study, profiling, clustering, co-expression analysis, and KEGG pathway analysis of primary metabolites and transcriptomes of Brassica rapa leaves and leaf mesophyll protoplasts were performed. Primary metabolite profiling indicated that the metabolism of B. rapa protoplasts was converted to catabolism. The number of down-regulated differentially expressed genes (DEGs) increased during protoplast isolation. An analysis of GO overexpression revealed activation of genes involved in catabolism and immune system processes and inactivation of chloroplasts and photosynthetic genes. KEGG pathway analysis showed that the activation of autophagy and the proteasome accounted for senescence-associated proteolysis during protoplast isolation. It also revealed activation of the genes involved in endoplasmic reticulum stress and disease resistance responses during the first three hours of isolation. This suggests that elicitor receptor-mediated signal transduction stimulated the pathogen-associated molecular patterns (PAMPs), which triggered immunity.There were more down-regulated than up-regulated transcription factors during the protoplast isolation. AT5G39610 (ANAC092, ATNAC2) gene expression was significantly activated throughout the entire period of protoplast isolation. ANAC092 was co-expressed with up-regulated AT5G26340 (STP13), which encodes a protein with high affinity, i.e., hexose-specific/H+ symporter activity. AT1G50030 encodes TOR (target of rapamycin) proteins, and the expression of TOR decreased during protoplast isolation. Ethylene and photosynthesis-related gene inhibition by glucose and sucrose promoted senescence-associated gene expression of protoplasts. Since a decrease in glucose down-regulates glucose TOR signaling, inactivated TOR signaling promotes catabolic reprogramming, senescence, and autophagy in protoplasts during protoplast isolation. We concluded that the initialization of protoplasts requires the blocking of these complicated crosstalk signaling pathways.
Heat-stress induced sesquiterpenes of Chrysanthemum nankingense attract herbivores but repel herbivore feeding
Authors: Wen Dian , Guan Yaqin , Chen Sumei , Chen Fadi , Liu Bin , Niinemets Ülo , Jiang Yifan 
Published: April 2022

Abstract
Plants are frequently exposed to heat stress as a result of global warming. Heat stress leads to a series of physiological responses including stress volatile elicitation, but how heat stress-induced volatile cues affect the behavior of herbivores is poorly understood. In this study, the polyphagous herbivore Spodoptera litura (tobacco cutworm, TCW) and perennial forb Chrysanthemum nankingense were selected as the model to elucidate the interactions between herbivore behavior and heat stress-induced plant physiological changes. Photosynthetic characteristics and volatile emissions were measured in C. nankingense control plants (25 ℃) and in plants exposed to moderate (35 ℃ for 3 h), and severe (45 ℃ for 3 h) heat stresses. Net photosynthetic rate (An) decreased by more than two-fold after exposure to 45 ℃ due to non-stomatal inhibition of photosynthesis. 45 ℃ treatment induced emissions of the monoterpene camphor and sesquiterpene (E)-β-caryophyllene. Exposure to 35 ℃ had minor effects on photosynthetic characteristics and did not induce terpene emissions. Using dual-choice olfactometer bioassays, we found that 45 ℃ treatment enhanced the attractiveness of the plants to TCW. Moreover, the leaf concentrations of 9 sesquiterpenes were increased and the feeding of TCW was strongly inhibited after 45 ℃ treatment compared with control plants. Taken together, our study highlights the impact of heat stress on the behavior of the herbivore mediated by the accumulation and emission of sesquiterpenes and suggests altered pest-host interactions under future warmer climates. Modulation of terpenoid emissions and contents should be considered in developing future ecological pest control strategies in agricultural fields.
Expression and Prognostic Significance of Stem Cell Marker CD133 in Survival Rate of Colon Cancer Patients
Authors: Tolooei Mohammad , Ehteram Hassan , Kashani Hamed Haddad 
Published: April 2022

Abstract
Background: Today, colon cancer is one of the most common types of gastrointestinal cancers CD133 as a known cancer stem cell marker has been found effective in cell proliferation and differentiation in various cancers, including colon. We aimed to investigate the relationship between the CD133 expression in colon cancer with prognostic factors and the survival rate of colon cancer patients by immunohistochemistry. Materials and Methods: Formalin- fixed-paraffinembedded (FFPE) tissues was taken from colon patients. Histopathology examination was done using hematoxylin and eosin staining. Immunohistochemistry was performed to determine CD133 expression. An association between CD133 expression and clinic pathological profile was then analyzed.Results: There was a statistically significant difference between CD133 protein expression and sex, cancer stage and lymphatic invasion (p=0.044, p=0.131 and p=0.002, respectively). However, no significant correlation was identified between the CD133 expression and other factors such as: age of CRC patients (p-value=0.267), tumor location (p-value=0.494), tumor differentiation grade (p-value=0.263), neural tissue invasion and 5 years’ survival (p value=0.054). Conclusion: CD133 is a useful predictive or prognostic biomarker for CRC in clinical assessment and may serve as a potential therapeutic target for CRC.
Clinical value of combination detection of direct antiglobulin test and serum albumin globulin ratio in severe hyperbilirubinemia caused by ABO hemolytic disease of the newborn: A single-center retrospective analysis
Authors: Hu Hongbing , Duan Ling , Chen Ping , Tu Na 
Published: April 2022

Abstract
Purpose To analyze clinical application of combination detection of direct antiglobulin test(DAT) and albumin globulin ratio(AGR) in severe hyperbilirubinemia caused by ABO hemolytic disease of the newborn(ABO-HDN). Methods The measurement of DAT, AGR and combination detection of DAT and AGR was done to predict severe ABO-HDN hyperbilirubinemia in 270 full-term infants based on whether the infants received transfusions of blood components. The infants were divided into three groups according to the results of DAT and ARG and compared the differences of phototherapy day and hospitalization day of the three groups. Results Of 270 cases enrolled in this study, 69 infants were DAT positive. ROC curve analysis showed that DAT cutoff value > ± with a sensitivity of 39.4% and a specificity of 83.9% and AGR cutoff value < 2.05 with a sensitivity of 54.1% and a specificity of 85.7%, the AUCs for DAT, AGR and combination detection of DAT and AGR were 0.621, 0.740 and 0.750 respectively. The phototherapy day and hospitalization day were significantly longer in group of AGR < 2.05 and DAT > ±than that of group of AGR < 2.05 and group of DAT > ±. Conclusions DAT and ARG could be early predictors for the severity ABO-HDN hyperbilirubinemia and combination detection of DAT and AGR could further increase its predictive value.
Lactiplantibacillus plantarum DLPT4 protects against cyclophosphamide-induced immunosuppression in mice by regulating immune response and intestinal flora
Authors: Song Yinglong , Sun Mengying , Ma Fenglian , Xu Dongxue , Mu Guangqing , Jiao Yang , Yu Ping , Tuo Yanfeng 
Published: April 2022

Abstract
In this study, the strain Lactiplantibacillus plantarum DLPT4 was investigated for the immunostimulatory activity in cyclophosphamide (CTX)-induced immunosuppressed BALB/c mice. L. plantarum DLPT4 was administered to BALB/c mice by oral gavage for 30 days, and CTX was injected intraperitoneally from 25th to 27th days. Intraperitoneally CTX injection caused the damage to the thymic cortex and intestines, and the immune dysfunction of the BALB/c mice. While L. plantarum DLPT4 oral administration exerted immunoregulating effects evidenced by increasing the serum immunoglobulin A (IgA), IgG and IgM levels, and reducing the expression of pro-inflammatory factors (IL-6, IL-1β and TNF-α) of the BALB/c mice with intraperitoneally CTX injection. The results of the BALB/c mice feces metagenome-sequencing showed that L. plantarum DLPT4 oral administration can regulate the intestinal microbial community by changing the ratio of Lactiplantibacillus and Bifidobacterium. Meanwhile, the abundance of carbohydrate enzyme (CAZyme), immune diseases metabolic pathways and p38 and AP-1 MAPK signaling pathways were enriched in the BALB/c mice with L. plantarum DLPT4 oral administration. In conclusion, L. plantarum DLPT4 administration ameliorated CTX-induced immunosuppression in BALB/c mice by regulating gut microbiota, and further influencing the abundance of carbohydrate esterase in the intestinal flora and increasing the immune metabolic activity. L. plantarum DLPT4 could be used as promising probiotics to regulate immune response.
The influence of agroclimatic factors on the abundance of Anastrepha ludens and A. obliqua (Diptera: Tephritidae), in “Barranqueño” mango (Mangifera indica).
Authors: Flores Aidé Monserrat Martínez , Rodríguez William David , Salcido Paulina Guadalupe Hernández , Mora Luis Antonio Guapo , Herrera Manuel Valderrama , Maya Rosa Navarrete 
Published: April 2022

Abstract
The objective of this research was to evaluate the influence of agroclimatic factors on the abundance of Anastrepha ludens and A. obliqua, in orchards of creole “Barranqueño” mango (Mangifera indica) in the municipalities of Amatitán, San Cristóbal de la Barranca, and Tequila (Jalisco, Mexico). Eighty-four multilure traps were established to sample specimens from May to September 2021.The most abundant species was A. ludens (98.7%). The greatest abundance of A. ludens occurred during June and July in all the municipalities, while for A. obliqua it was in June. The index of flies captured per trap per day had values greater than 0.010, which showed that the study area has a high prevalence of the pest. There is a significant correlation (P-value = 0.001) between the abundance of A. ludens with precipitation (19.30-260.60 mm) and minimum temperature (16.7–22.20°C). The abundance of A. obliqua was very low (504 individuals in total) and did not correlate with any variable. The altitude range (842-1151.75 m asl) of the study area explains the differences in the abundance of these species. These results are evidence that precipitation and the survival of adults of these species, does not have a cause-effect relationship, and that population fluctuations among both Anastrepha species may be correlated with altitude.
The role of dual-task, self-efficacy and sensation seeking in high and low risk climbing sports
Authors: Towey Giulia Emma , Andricciola Federica , Fabio Rosa Angela 
Published: April 2022

Abstract
High-risk sports are influenced by contextual features and by personality traits such as self-efficacy and sensation seeking. The aim of this study was to investigate if dual-task, self-efficacy and sensation seeking influence sport performance in high and low-risk climbing disciplines. Sixty-nine climbers who practice low-risk (N = 34) and high-risk climbing (N =35) completed questionnaires on generalized self-efficacy and sensation seeking. The subjects carried out respectively the low or the high-risk tasks twice: with and without dual-task demands. The parameters of sport performance were: the total length covered and the number of mistakes. Results showed that climbers with high levels of sensation seeking demonstrated also high levels of self-efficacy, nevertheless, these personality traits do not affect directly the sport performance. Moreover, dual-task condition influences sport performance by worsening it. Results were discussed in light of the interference that concurrent demands may have on sport performance.
Extreme Non-Wetting of Condensation-Induced Droplets on Smooth Monolayer Suspended Graphene
Authors: Wang Haidong , Orejon Daniel , Song Dongxing , Zhang Xing , McHale Glen , Takamatsu Hiroshi , Takata Yasuyuki , sefiane Khellil 
Published: April 2022

Abstract
Superhydrophobicity has only ever been achieved by a combination of chemical hydrophobicity and surface topography due to our inability to attain complete non-wetting on the smooth surface of any known material. Here, we report extreme non-wetting of condensation-induced droplets with contact angles approaching 180° on a smooth surface using suspended monolayers of graphene experimentally. Such extreme non-wetting droplets are reported exclusively on suspended monolayer graphene open to the water vapour saturated environment on both sides. Simultaneous observations of droplets condensing on monolayer and multilayer supported and suspended graphene demonstrates that this extreme non-wetting behaviour is unique to suspended monolayer graphene. These results anticipate that interactions between liquid molecules across a suspended monolayer isolated from a bulk substrate may induce extreme non-wetting beyond that possible on smooth hydrophobic or atomically flat supported monolayer surfaces.
Controller and loop gains are decreased by oropharyngeal obstruction in pediatric obstructive sleep apnea syndrome
Authors: Bokov Plamen , Boujemla Imene , Matrot Boris , Spruyt Karen , Gallego Jorge , Delclaux Christophe 
Published: April 2022

Abstract
Instable ventilatory control is an endotypic trait of obstructive sleep apnea syndrome (OSAS). The study aimed to evaluate the relationships between the anatomical compromise of the upper (oro- and naso-pharynx) and lower airways and ventilatory control (measured by chemical loop gain) in otherwise healthy children suffering from moderate to severe OSAS (apnea hypopnea index ≥ 5/hour). The children underwent ear, nose and throat examination, measurement of impedance of the respiratory system that allowed characterizing peripheral lung mechanics using the extended Resistance-Inertance-Compliance model. Physiologically constrained analytical model based on tidal breathing analysis allowed the computation of steady-state plant gain (PG0), steady-state controller gain (CG0) and steady-state loop gain (LG0). Medium-frequency components of feedback control system were then deduced. Fifty children (median age 11.2 years, 17 females) were enrolled. Oropharyngeal obstruction was associated with decreased CG0 (0.6 [0.2; 1.0] vs 1.5 [0.5; 6.6] L.s− 1.mmHg− 1, p = 0.038) and LG0 (0.4 [0.2; 1.1] vs 1.2 [0.4; 9.3], p = 0.027), while nasal obstruction did not modify ventilatory control parameters. In a multivariate analysis Medium-Frequency PG was negatively related to minute ventilation and respiratory system compliance. Both upper (tonsil hypertrophy) and lower (compliance of respiratory system) airways are linked to ventilatory control in children with moderate to severe OSAS.
Distinct neural activation pattern of age on subcomponents of inhibitory control: a fMRI meta-analysis
Authors: Long Jixin , Song Xiaoqi , Wang You , Wang Chanyu , Huang Ruiwang , Zhang Ruibin 
Published: April 2022

Abstract
Inhibitory control (IC) is a fundamental cognitive function showing age-related change across the healthy lifespan. Since different cognitive load of two subcomponents of IC was existed, that is cognitive inhibition and response inhibition, there are regions differentially activated during two subcomponents of IC. In this study, we aimed to characterize whether there is distinct age-related activation pattern in these two subcomponents. A total of 278 fMRI articles were included in the current analysis. Multilevel kernel density analysis was used to derive the brain activation under each subcomponent of IC. Contrast analyses were conducted to capture the distinct activated brain regions for two subcomponents and meta-regression analyses were performed to obtain brain regions with distinct age-related activation patterns in two subcomponents of IC. The results showed that the right inferior frontal gyrus and the bilateral insula were activated during two IC subcomponents. Contrast analyses revealed stronger activation in the superior parietal lobule during cognitive inhibition, whereas greater activation was observed during response inhibition primarily in the right inferior frontal gyrus, bilateral insula and angular gyrus. Furthermore, regression analyses showed that activation of the left anterior cingulate cortex, left inferior frontal gyrus, bilateral insula, and left superior parietal lobule increased and decreased with age during cognitive inhibition and response inhibition, respectively. Results showed distinct activation patterns of aging for the two subcomponents of IC, which may be related to the differential cognitive loads of the two subcomponents of IC. These findings may help to enhance our knowledge of age-related changes in activation patterns of IC.