bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic activity of pomegranate rind extract and Zn (II) against Candida albicans under planktonic and biofilm conditions, and a mechanistic insight based upon intracellular ROS induction
Authors: Celiksoy Vildan , Moses Rachael L. , Sloan Alastair J. , Moseley Ryan , Heard Charles M. 
Published: July 2022

Abstract
Candida albicans is an opportunistic pathogen, which causes superficial infection and can lead to mortal systemic infections, especially in immunocompromised patients. The incidence of C. albicans infections is increasing and there are a limited number of antifungal drugs used in treatment. Therefore, there is an urgent need for new and alternative antifungal drugs. Pomegranate rind extract (PRE) is known for its broad-spectrum antimicrobial activities, including against C. albicans and recently, PRE and Zn (II) have been shown to induce synergistic antimicrobial activity against various microbes. In this study, the inhibitory activities of PRE, Zn (II) and PRE in combination with Zn (II) were evaluated against C. albicans. Antifungal activities of PRE and Zn (II) were evaluated using conventional microdilution methods and the interaction between these compounds was assessed by in vitro checkerboard and time kill assays in planktonic cultures. The anti-biofilm activities of PRE, Zn (II) and PRE in combination with Zn (II) were assessed using confocal laser scanning microscopy, with quantitative analysis of biofilm biomass and mean thickness analysed using COMSTAT2 analysis. In addition, antimicrobial interactions between PRE and Zn (II) were assayed in terms reactive oxygen species (ROS) production by C. albicans. PRE and Zn (II) showed a potent antifungal activity against C. albicans, with MIC values of 4 mg/mL and 1.8 mg/mL, respectively. PRE and Zn (II) in combination exerted a synergistic antifungal effect, as confirmed by in vitro checkerboard and time kill assays. PRE, Zn (II) and PRE and Zn (II) in combination induced significant reductions in biofilm biomass, but only PRE caused a substantial reduction in biofilm mean thickness. PRE and Zn (II) in combination caused the highest levels of ROS production by C. albicans, in both planktonic and biofilm forms. PRE, Zn (II) and PRE and Zn (II) in combination possess potent antifungal activity against C. albicans in both planktonic and biofilm forms. PRE and Zn (II) further exhibited a synergistic antifungal activity via the induction of excessive ROS accumulation in C. albicans under both planktonic and biofilm forms, which may contribute to their antifungal effects.
Impact of health education on controlling and preventing the transmission of cystic echinococcosis
Authors: Cui Qianqian , He Yiwei , Wen Buyu , Hu Zengyun , Li Shizhu 
Published: July 2022

Abstract
Background: Cystic echinococcosis (CE), which is one of the most severe helminth zoonosis, can seriously threaten human health and hinder the development of livestock industry. Health education has widely disseminated and does play an important role in controlling and eliminating the spread of CE. Assessing the crucial factors of health education are essential for optimizing health education strategies to mitigate this disease.Methods: A compartment model involving health education was formulated. The next generation matrix approach was adopted to calculate the basic reproduction number R0, and the Lyapunov Direct method was used to investigate the global asymptotically stability of equilibria. Partial rank correlation coefficient (PRCC), a global sensitively analysis method, was employed to determine the key factors of R0. Numerical simulations were further given to evaluate the effects of health education on the transmission of CE.Results: The dynamics of the model is completely determined by the basic reproduction number R0. If R0<1, the disease goes extinct and if R0>1 the disease persists. The positive effect of health education was theoretically verified. And the disposal of infected sheep entrails (deeply burial and no feeding dogs) are the most important factors that affect the value of R0 and the spread of CE.Conclusions: Health education is an effective strategy to prevent and control the spread of CE. The disposal of infected sheep entrails (especially, deeply burial and no feeding dogs) should be emphasized in health education products. This can strengthen herdsmen's awareness of disposal infected sheep entrails and then mitigate the spread of CE.
Oxidation driven reversal of PIP2-dependent gating in GIRK2 channels
Authors: Lee Sun-Joo , Nichols Colin 
Published: July 2022

Abstract
Physiological activity of G protein gated inward rectifier K+ (GIRK, Kir3) channel activity is dynamically regulated by three key ligands, phosphoinositol-4,5-bisphosphate (PIP2), Gβγ, and Na+, which underlies cellular electrical response to multiple hormones and neurotransmitters in myocytes and neurons. In a reducing environment, matching that inside cells, purified GIRK2 (Kir3.2) channels demonstrated low basal activity, and appropriate sensitivity to the above ligands. However, under oxidizing conditions, anomalous behavior emerged, including rapid loss of agonist dependent activation, and high basal activity in the absence of any agonists that was paradoxically inhibited by PIP2. Mutagenesis identified two cysteine residues (C65 and C190) as being responsible for the loss of agonist dependent activation and the elevated basal activity, respectively. The results explain anomalous findings from earlier studies and illustrate the potential pathophysiologic consequences of oxidation on GIRK channel function, as well as providing insight to reversed ligand-dependence of gating in related Kir and KirBac channels.
Effects of Fatigue, Rumination Thinking and Sleep Quality on the Hopelessness of Malignant Tumor Patients‘ Family Caregiver
Authors: Cao Zhaoming , Wang Yingchun , ZHANG Huijun 
Published: July 2022

Abstract
Introduction:The heavy care burden, the long-term dependence of the malignant tumor patients, and the changing social roles may put family caregivers at high risk for mental disorders even hopelessness. This study aimed to explore the relationship between fatigue, sleep quality, rumination thinking and hopelessness, and to provide suggestions for maintaining the physical and mental health of caregivers. Results:The family caregivers of patients with malignant tumor had a higher degree of hopelessness, and fatigue, sleep quality and rumination were positively correlated with the sense of hopelessness (r = 0.483–0.906; r = 0; P < 0.05); Fatigue and sleep quality can indirectly affect hopelessness through rumination thinking, and hopelessness has a direct effect on sleep. Discussion:There are multiple action paths between fatigue and sleep quality, rumination thinking and hopelessness, we can maintain family caregivers‘ physical and mental health and avoid hopelessness by improving their sleep quality, alleviating their fatigue and psychological counselling. Methods:A total of 536 families‘ caregivers were investigated,Family caregivers of malignant tumor patients who met the inclusion criteria in three Grade-A hospitals in Liaoning Province were investigated with Beck hopelessness scale (BHS), fatigue Scale-14 (FS-14), Nolen-Hoeksema ruminative responses scale (RRS) and Pittsburgh sleep quality index (PSQI), SPSS 26.0 was used to analyze the data, and Amos 22.0 was used to fit the structural equation model.
Therapeutic effects of Eucommia water extract on periodontitis in experimental rats
Authors: Wang Yueyue , Fan Qin , Xu Yanglong , Zeng Fengjiao , Liu Xia , Zhao Dan , Zhang Lei , Bai Guohui 
Published: July 2022

Abstract
Herein, we evaluated the potential therapeutic effects of water extracts from Eucommia on periodontitis in experimental rats. We ligated the maxillary second molars of SD rats with 4.0 silk threads and locally smeared Porphyromonas gingivalis to induce gingivitis and periodontitis. After successfully establishing the model, we exposed the rats to Eucommia water extracts through topical smearing and intragastric administration and evaluated the therapeutic effect of the extracts on gingivitis (for a 2 week treatment period) and periodontitis (over 4 weeks). We recorded the alveolar bone resorption levels and analyzed histopathological sections of the periodontal tissue, molecules related to periodontal oxidative stress, and periodontal inflammatory factors to understand the feasibility of Eucommia in treating gingivitis and periodontitis. Results showed reduced damage to the periodontal tissue after treatment with extracts, indicating that Eucommia has a positive effect in treating gingivitis and periodontitis in experimental rats. These findings are expected to provide the foothold for future research on secondary metabolites derived from Eucommia and guide the development of novel approaches for preventing and treating periodontal disease.
Research on milling chatter identification of thin-walled parts based on incremental learning and multi-signal fusion
Authors: Zhao Mingwei , Yue Caixu , Liu Xianli 
Published: July 2022

Abstract
Chatter is a kind of self-excited vibration that often occurs in the milling process of thin-walled parts. It has become the main factor restricting production efficiency and quality. Due to the occurrence of chatter, the signal becomes more complex and unstable. To realize milling chatter detection of thin-walled parts, a method based on variational modal decomposition (VMD) and nonlinear dimensionless index is proposed by using the multi-sensor signal fusion and complementarity method to analyze the characteristics of signals from the time-frequency domain. Firstly, VMD decomposes the force signal and acceleration signal to obtain a series of intrinsic mode function (IMF) components. When chatter occurs, the energy is transferred to the chatter frequency band. Each IMF signal's nonlinear energy entropy (EE) is extracted to construct the feature vector. A chatter identification model is established based on multi-sensor signal fusion and support vector machine. To solve the problem of model updating, supervised learning and unsupervised learning are combined to provide a method for chatter detection.
Photoluminescence and Optical Properties of CuO Thin Films Deposited via Spray Pyrolysis: Influence of Substrate Temperature
Authors: Asl Hassan Zare , Rozati Seyed Mohammad 
Published: July 2022

Abstract
CuO thin films were spray-deposited onto a glass substrate and the deposition temperature was varied from 450 to 550 ℃ with 25 ℃ steps. X-ray diffraction (XRD) and field-emission scanning electron microscopy (FESEM) techniques were employed to respectively record and study the structural and morphological characteristics of the resulting CuO thin films. The 450 ℃ deposition temperature was found to be the optimum condition for crystal growth. However, the FESEM images revealed that the grain size increases with raising the deposition temperature. To investigate the optical properties, the transmittance and reflectance data of the resulting films were recorded, and their optical constants, including absorption coefficient, band gap, Urbach energy, refractive index, extinction coefficient, and real and imaginary parts of the dielectric constant were calculated. All the prepared CuO thin films exhibited band gap values around 2.00 eV, which slightly declined with increasing the substrate temperature. The general trend of real and imaginary parts of the dielectric constant was increment and decrement, respectively, with increasing the substrate temperature. The spray-deposited CuO thin films were also excited at 486 nm, and the resulting photoluminescence (PL) spectra were recorded at room temperature. Three main and three shoulder peak emissions were detected in the recorded PL of all CuO thin films. The lowest peak intensity was related to the CuO thin film deposited at 500 ℃.
A simple Schiff base as fluorescent probe for detection of Al 3+ in aqueous media and its application in cells imaging
Authors: Sang Fuming , Wang Weijie , Pan Jianxin , Shi Huahua , Zhao Yan 
Published: July 2022

Abstract
A novel fluorescence probe for the detection of Al3+ was developed based on methionine protected gold nanoclusters (Met-AuNCs). A fluorescent Schiff base (an aldimine) is formed between the aldehyde group of salicylaldehyde (SA) and the amino groups of Met on the AuNCs, and developed for selective detection of Al3+ in aqueous solution. Al3+ can strongly bind with the Schiff base ligands, accompanied by the blue-shift and an obvious fluorescence emission enhancement at 455 nm. The limits of detection (LODs) of the probe are 2 pmol L− 1 for Al3+. Moreover, the probe can successfully be used in fluorescence imaging of Al3+ in living cells (SHSY5Y cells), suggesting that the simple fluorescent probe has great potential use in biological imaging.
Co-culture of the Potential First Reported Streptomyces flavalbus of Sonneratia alba from Ujung Piring, Jepara, Indonesia
Authors: Azhar Nuril , Yudiati Ervia 
Published: July 2022

Abstract
Background: Marine Actinobacteria from mangrove are rich in novel secondary metabolites resource as a promising antibacterial agent. The silent gene assessment by gen co-cuture fermentation of Actino-Actino, Actino-other bacteria and Actino-fungi is an alternative approach to create the novel product. This research aims to discover and identify the potential Actinobacteria from the mangrove sediment and root with co-culture as an antibacterial agent.Results: 28 strains of Actinobacteria were found, composed of 17 strains from the sediment and 11 strains from the endophyte root of Sonneratia alba. Out of 580 fermentation, 540 fermenter co-cultures and 34 single fermenter cultures were assessed. Twenty-one pairs of co-culture were resulted positively against Staphylococcus aureus, and 16 pairs were positive against Pseudomonas aeruginosa. The highest clearance zone against S. aureus was obtained from 10 (Actino)-Aspergillus fumigatus (dead body) extract (21.57±0.90 mm) while against P. aeruginosa was resulted from co-culture strains 3/37 extract (19.18±0.80 mm). Strains 41 was identified as Streptomyces flavalbus (98.07%) with accession number MZ540332. This is the first report of Streptomyces flavalbus, a novel species from mangrove sediments, and the second one from natural habitat. Other Actinobacterias (6, 10, and 37) strains were identified as Streptomyces albogriseolus (99.79%), Streptomyces labedae (99%), and Streptomyces rochei (93.52%).Conclusion: It is concluded that co-culture is a valuable strategy to develop the new Actinobacteria product as a powerful antibacterial agent.
Exploring the eco-efficiency of cultivated land utilization and its influencing factors in black soil region of Northeast China under the goal of reducing pollution emission and increasing carbon sink
Authors: Feng Lin , Lei Guoping , Nie Ying 
Published: July 2022

Abstract
To realize maximum benefits and minimize environment pollution, the eco-efficiency of cultivated land utilization(ECLU) is becoming a vital indicator in weighing the rationality of regional land use. Scientific analysis of spatial-temporal pattern variations, as well as factors influencing the ECLU, is of great significance to cultivated land protection, economic development and ecological environment protection in black soil region of Northeast China, and has become a global strategic issue related to the sustainable development. However, assessments of the indicators are still incomplete, the lack of information may inhabit planning guideline for the sustainable development of cultivated land resources. Thus, this study attempts to fill the gaps by incorporating the net carbon sink and non-point source pollution emissions into the measurement framework of ECLU. The super-efficiency SBM model with undesirable output and the Malmquist-Luenberger(ML) index were used to measure the ECLU and changes in the total factor productivity(TFP) of cultivated land use in the Songnen Plain from 1989 to 2019. Moran index and LISA clustering was used to reveal spatital correlation of ECLU, and Kernel density estimation and trend surface analysis maps were drawn to analyze variation tendency of the ECLU. Geographical detector model was employed to further analyze the influencing factors of ECLU. The study results revealed that:(1)The ECLU showed an overall development trend of "rising first and then declining", all of which were below the frontier efficiency. (2)The polarization of ECLU is prominent, and the ECLU had a significant heterogeneity and spatital correlation. (3)The TFP showing an increasing trend, technological progress is the main driving force to promote the progress of TFP, while the technological efficiency is the bottlenecks for the increasing the TFP of the cultivated land use. (4)According to the magnitude of influencing factor, increasing agricultural science and technology investments, lowering carbon emission, and controlling agrochemical investment could significantly enhance the ECLU. These findings have important implications for promoting high-efficient, low-carbon utilization of cultivated land resources and sustainable regional development in black soil region of Northeast China.
Transformation of amorphous aluminum oxide in the catalytic dehydration reaction of aromatic alcohol
Authors: Boretskaya Avgustina , Farid Mostafa Esmail , Egorova Svetlana , Lamberov Alexander 
Published: July 2022

Abstract
The wide use of aluminum oxides as catalysts in petrochemicals and oil refining applications necessitates determination of factors affecting the catalytic systems efficiency. However, there are no studies showing the amorphous phase effect on the composition of aluminum oxide catalysts and the catalytic performance. At the same time, the uncontrolled presence of an amorphous phase in aluminum oxide catalyst can significantly worsen the catalyst efficiency. This paper shows for the first time, the transformation of amorphous aluminum oxide during the reaction of vapor-phase dehydration of aromatic alcohol, which leads to a decrease in conversion from 88 (for fresh) to 64% (after regeneration). Crystallization of amorphous aluminum oxide by high-temperature treatment may be impractical, due to a significant decrease in the catalyst textural characteristics.
Decoupling of K isotope from Sr-Nd isotope in the Saima alkaline complex, NE China: interactions of cratonic roots and asthenosphere
Authors: Gao Nan , Zhu Dan , Xu Yingkui , Luo Jincheng , Li Yang , Li Xiongyao , Liu Jianzhong 
Published: July 2022

Abstract
We report high-precision K isotopes, apatite U-Pb ages, whole-rock elements, and Sr-Nd isotopes of Saima nepheline syenite in the North China Craton. Trace element and Sr-Nd-Hf-O isotope data indicate the presence of subducting sediments in source region, while K isotopic compositions showed a narrow range between –0.54 ‰ and –0.31 ‰, with an average of –0.41 ± 0.06 ‰, identical to the value of asthenosphere. The nearly identical K isotopic compositions are low probability events compared with K isotopic compositions of the island arc lavas reported previously (–1.55 ‰ to +0.2 ‰). Although crustal contamination is consistent with Sr-Nd-K isotopic data, alternatively we propose the isotopic data also reconcile to the interaction between cratonic roots and underlying convective asthenosphere, if this interaction is over prolonged periods of time. Numerical simulations successfully reproduced the observed data, if the metasomatized lithospheric mantle root, the source of the Saima, occurred 500 Ma ago. Our study reveals that the isotopic compositions of fast-diffusion components in a metasomatized lithospheric mantle by ancient subducting melts can be effectively homogenized by convective asthenosphere through diffusion in a long-time interval.
Taxonomic assessment of two wild house mouse subspecies using whole-genome sequencing
Authors: Lawal Raman Akinyanju , Mathis Verity L. , Barter Mary E. , Charette Jeremy R. , Garretson Alexis , Dumont Beth L. 
Published: July 2022

Abstract
House mice (Mus musculus) are comprised of three primary subspecies. A large number of secondary subspecies have also been suggested on the basis of divergent morphology and molecular variation at limited numbers of markers. While the phylogenetic relationships among the primary M. musculus subspecies are well-defined, the relationships among the secondary subspecies, and their relationships to primary subspecies, remain poorly understood. Here, we integrate de novo genome sequencing of museum-stored specimens of house mice from one secondary subspecies (M. m. bactrianus) and publicly available genome sequences of house mice previously characterized as M. m. helgolandicus, with whole-genome sequences from diverse representatives of the three primary house mouse subspecies. We show that mice assigned to the secondary M. m. bactrianus and M. m. helgolandicus subspecies are not genetically differentiated from the contemporary M. m. castaneus and M. m. domesticus, respectively. Overall, our work suggests that the M. m. bactrianus and M. m. helgolandicus subspecies are not well-justified taxonomic entities, emphasizing the importance of leveraging whole-genome sequence data to inform subspecies designations. Additionally, our investigation has established experimental procedures for generating whole-genome sequences from air-dried museum specimens and provides key genomic resources to inform future genomic investigations of wild mouse diversity.
Enhanced recovery after surgery in EMS lithotripsy for percutaneous nephrolithotomy: a retrospective cohort study
Authors: Gao Meng , Zhu Zewu , Liu Minghui , Chen Jinbo , Chen Hequn , Zeng Feng 
Published: July 2022

Abstract
Background
The enhanced recovery after surgery (ERAS) protocols for many urological procedures are well developed, but application of an ERAS protocol in PCNL is still limited. The objective of this study was to investigate postoperative recovery following PCNL combined with EMS Trilogy lithotripsy using our institutional ERAS protocols.
Materials and Methods
From January 2018 to July 2021, a perioperative ERAS protocol was applied in 104 patients who underwent PCNL combined with EMS Trilogy. A matched cohort of 104 patients for whom a traditional protocol was used between 2012 and 2018 was selected, and the stone-free rate, incidence of complications, time from surgery to first flatus, postoperative bed rest time, postoperative hospitalization and expenses were compared between the two groups.
Results
The mean time from surgery to first flatus was 14.8 hours in the ERAS cohort and 26.4 hours in the control group (P < 0.001). Patients in the ERAS group also had a significantly shorter postoperative bed rest time (23.8 vs. 47.2 hours, P < 0.001) and postoperative hospitalization (4.49 vs. 6.64 d, P < 0.001). The average hospitalization cost for the ERAS group was 8.5% lower than that for the traditional group (19749 vs. 21584 CNY, P < 0.001).
Conclusions
Patients in the ERAS group had earlier anal exhaust and out-of-bed times, fewer hospitalization days, lower hospitalization costs, and no increased risks of perioperative complications or residual stones.
Trial registration:
26/11/2021, Chinese Clinical Trial Registry (ChiCTR2100053652).