bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of a split‐Cre system for high‐capacity adenoviral vector amplification
Authors: Gonzalez‐Aparicio Manuela , Bunuales Maria , de Landazuri Iñaki Ortiz , Prieto Jesus , Hernandez‐Alcoceba Ruben 
Published: December 2022

AbstractBackground and AimsHigh‐capacity adenoviral vectors (HC‐AdV) show extended DNA payload and stability of gene expression in vivo due to the absence of viral coding sequences. However, production requires methods to trans‐complement viral proteins, usually through Helper Viruses (HV). The Cre/loxP system is frequently employed to remove the packaging signal in HV genomes, in order to avoid their encapsidation. However, chronic exposure to the Cre recombinase in packaging cells is detrimental. We have applied the dimerizable Cre system to overcome this limitation.Methods and ResultsCre was split in two fragments devoid of recombinase function (N‐terminal 244 and C‐terminal 99 amino‐acids). In one version of the system, interaction with both moieties was favored by rapamycin‐dependent heterodimerization domains (DiCre). Other version contained only Cre sequences (oCre). We generated packaging cells and HVs expressing the complementary fragments and studied their performance for HC‐AdV production. We found that both conformations avoided interference with the growth of packaging cells, and the oCre system was particularly suitable for HC‐AdV amplification.ConclusionsThe split‐Cre system improves the performance of packaging cells and can reduce the time and cost of HC‐AdV amplification up to 30% and 15%, respectively. This may contribute to the standardization of HC‐AdV production.
Capacitance spectroscopy enables real‐time monitoring of early cell death in mammalian cell culture
Authors: Wu Suyang , Ketcham Stephanie A , Corredor Claudia C. , Both Douglas , Drennen James K. , Anderson Carl A. 
Published: December 2022

AbstractBackground/AimsPrevious work developed a quantitative model using capacitance spectroscopy in an at‐line setup to predict the dying cell percentage measured from a flow cytometer. This work aimed to transfer the at‐line model to monitor lab‐scale bioreactors in real‐time, waiving the need for frequent sampling and enabling precise controls.Methods and ResultsDue to the difference between the at‐line and in‐line capacitance probes, direct application of the at‐line model resulted in poor accuracy and high prediction bias. A new model with a variable range and offering similar spectral shape across all probes was first constructed, improving prediction accuracy. Moreover, the global calibration method included the variance of different probes and scales in the model, reducing prediction bias. External parameter orthogonalization, a preprocessing method, also mitigated the interference from feeding, which further improved model performance. The root‐mean‐square error of prediction of the final model was 6.56% (8.42% of the prediction range) with an R2 of 92.4%.ConclusionThe culture evolution trajectory predicted by the in‐line model captured the cell death and alarmed cell death onset earlier than the trypan blue exclusion test. Additionally, the incorporation of at‐line spectra following orthogonal design into the calibration set was shown to generate calibration models that are more robust than the calibration models constructed using the in‐line spectra only. This is advantageous, as at‐line spectral collection is easier, faster, and more material‐sparing than in‐line spectra collection.
Collagen scaffolds derived from bovine skin loaded withMSCoptimizedM1macrophages remodeling and chronic diabetic wounds healing
Authors: Liu Hengdeng , Yang Ronghua , Zhao Shixin , Zhou Fei , Liu Yiling , Zhou Ziheng , Chen Lei , Xie Julin 
Published: December 2022

AbstractOwing to the persistent inflammatory microenvironment and unsubstantial dermal tissues, chronic diabetic wounds do not heal easily and their recurrence rate is high. Therefore, a dermal substitute that can induce rapid tissue regeneration and inhibit scar formation is urgently required to address this concern. In this study, we established biologically active dermal substitutes (BADS) by combining novel animal tissue‐derived collagen dermal‐replacement scaffolds (CDRS) and bone marrow mesenchymal stem cells (BMSCs) for the healing and recurrence treatments of chronic diabetic wounds. The collagen scaffolds derived from bovine skin (CBS) displayed good physicochemical properties and superior biocompatibility. CBS loaded with BMSCs (CBS‐MCSs) could inhibit M1 macrophage polarization in vitro. Decreased MMP‐9 and increased Col3 at the protein level were detected in CBS‐MSCs‐treated M1 macrophages, which may be attributed to the suppression of the TNF‐α/NF‐κB signaling pathway (downregulating phospho‐IKKα/β/total IKKα/β, phospho‐IκB/total IκB, and phospho‐NFκB/total NFκB) in M1 macrophages. Moreover, CBS‐MSCs could benefit the transformation of M1 (downregulating iNOS) to M2 (upregulating CD206) macrophages. Wound‐healing evaluations demonstrated that CBS‐MSCs regulated the polarization of macrophages and the balance of inflammatory factors (pro‐inflammatory: IL‐1β, TNF‐α, and MMP‐9; anti‐inflammatory: IL‐10 and TGF‐β3) in db/db mice. Furthermore, CBS‐MSCs facilitated the noncontractile and re‐epithelialized processes, granulation tissue regeneration, and neovascularization of chronic diabetic wounds. Thus, CBS‐MSCs have a potential value for clinical application in promoting the healing of chronic diabetic wounds and preventing the recurrence of ulcers.
Effects of grain storage time and storage temperature on flour quality parameters of “5 + 10” wheat cultivar and “2 + 12” wheat cultivar
Authors: Liu Hongfei , Zhang Yingquan , Don Clyde , Zhang Bo 
Published: December 2022

AbstractBackgrounds and ObjectivesPhysicochemical changes that take place inside the wheat kernel during storage make the quality stable. Storage temperature will affect the different mechanisms of changes. The newly harvested “2 + 12” wheat cultivar (H4399) and “5 + 10” wheat cultivar (XM26) grains were stored under ambient temperature and 4°C for 120 days, respectively, and sampled for grinding every 30 days.FindingsWith the extension of storage time, the content of crude protein and glutenin macropolymer (GMP), Gʹ and G" of GMP gel, maximum resistance, and extensibility of both varieties increased to higher value and then decreased slightly. The higher value appeared at 90 or 120 days under ambient temperature, and at 30 or 60 days under 4°C, approximately 30–60 days earlier than under ambient temperature. During storage, the relative increasing of protein and GMP content, sedimentation index, G", tan δ, stability, maximum resistance, and extendibility of dough of “2 + 12” wheat cultivar (H4399) were bigger than “5 + 10” wheat cultivar (XM26). The relative increasing of Gʹ, water absorption, development time of dough were smaller than “5 + 10” wheat cultivar (XM26).ConclusionsIt is concluded that low‐temperature storage can accelerate protein aggregation in wheat grains and shorten the time to steady state. Increasing in GMP stiffness and dough development time is more sensitive to postharvest storage in a “5 + 10” wheat cultivar (XM26) versus a “2 + 12” wheat cultivar (H4399).Significance and NoveltyThe changes of protein physicochemical properties of different varieties under different storage conditions explained the main mechanism of protein aggregation during wheat storage.
Engineering a Medium‐chain Alcohol Dehydrogenase for Efficient Synthesis of (S)‐N−Boc‐3−pyrrolidinol by Adjusting the Conformational Dynamics of Loops
Authors: Ye Wenjie , Zhang Yuxin , Wang Yilin , Xie Jingwen , Liu Yan , Yang Lin , Wang Hualei , Wei Dongzhi 
Published: December 2022

AbstractLoops typically allosterically communicate with active sites, and their conformational dynamics can affect the catalytic properties of enzymes. Herein, by manipulating the loop conformational dynamics via adjusting loop‐loop interactions, a medium‐chain alcohol dehydrogenase (Syn94) was engineered to enhance the activity without enantioselectivity reduction. Syn94, from Synechocystis sp. PCC 6803 was identified, and it demonstrated good enantioselectivity (>99.9 % ee) for synthesizing (S)‐N−Boc‐3−pyrrolidinol ((S)‐Boc−PL), a key intermediate of darifenacin, but with low catalytic efficiency. To enhance its activity, three regions for loop‐loop interactions near the active pocket were identified for mutagenesis. After the iteration of two effective sites, the best mutant, S89T/P282R, with a 94.5‐fold enhancement in catalytic efficiency, was successfully obtained. By establishing a co‐expression system of S89T/P282R and glucose dehydrogenase (GDH), up to 1.0 M (180.2 g/L) substrate could be completely reduced to (S)‐Boc−PL within 7 h, with 617 g/L/d space‐time‐yield. Molecular dynamics simulations revealed that the enhanced activity was related to the stabilization of two loops, D41‐V58 and W87‐T112.
Circularly polarized and total luminescence as probes of nucleation and growth in chiral nanocrystals
Authors: Schwartz Gal , Hananel Uri , Markovich Gil 
Published: December 2022

AbstractNucleation of crystals as well as their growth is difficult to study experimentally. We have recently demonstrated that chiral Eu3+‐doped terbium phosphate nanocrystals are an interesting system for studying nanocrystal formation mechanisms and chiral symmetry breaking, occurring during their formation, directed by chiral ligands, such as tartaric acid. In this paper, we show how simultaneous, in situ monitoring of both total emission intensity and circularly polarized luminescence magnitude and sign versus time during nanocrystal formation provides considerable information on the mechanisms of nanocrystal nucleation and growth. Specifically, we show that the presence of tartaric acid leads to the formation of chiral prenucleation clusters, which deterministically transform into nanocrystals of a specific handedness. Additionally, we demonstrate that both unseeded and seeded nanocrystal syntheses behave differently mechanistically and that the addition of seed nanocrystals catalyses both enantio‐specific (also called secondary nucleation) as well as nonspecific nucleation.
PROTOCOL: Exploring education to support vaccine confidence amongst healthcare and long‐term care staff amidst the COVID‐19 pandemic: A protocol for a living scoping review
Authors: Reed Anna C. , Murmann Maya , Ramzy Amy , Scott Mary , Skidmore Becky , Welch Vivian , Hsu Amy T. 
Published: December 2022

AbstractDespite the demonstrated effectiveness of vaccines, varying levels of hesitancy were observed among healthcare and long‐term care workers, who were prioritized in the roll out of COVID‐19 vaccines due to their high risk of exposure to SARS‐CoV‐2 transmission. However, the evidence around the measurable impact of various educational interventions to improve vaccine confidence is limited. The proposed scoping review is intended to explore any emerging research and experiences of delivering educational interventions to improve COVID‐19 vaccine confidence among health and long‐term care workforces. We aim to identify characteristics of both informal and formal educational interventions delivered during the pandemic to support COVID‐19 vaccine hesitancy. Using the guidance outlined by the Joanna Briggs Institute, we intend to search five databases including, Ovid MEDLINE and Web of Science, as well as grey literature. We will consider all study designs and reports in an effort to include a breadth of sources to ensure our review will capture preliminary evidence, as well as more exploratory experiences of COVID‐19 vaccine education delivery. Articles will be screened by three reviewers independently and the data will be charted, and results described narratively.
Novel predictive estimators using ranked set sampling
Authors: Bhushan Shashi , Kumar Anoop 
Published: December 2022

AbstractThis article suggests some novel logarithmic predictive estimators using ranked set sampling for the computation of population mean. The bias and mean square error of the proposed predictive estimators (PPEs) are determined up to the approximation of order one. The performance of the PPEs is examined regarding the contemporary existing predictive estimators (EPEs) utilizing simple random sampling (SRS) and ranked set sampling (RSS) and the efficiency conditions are obtained. Subsequently, a comprehensive computational examination is accomplished based on natural and artificially rendered symmetric and asymmetric populations to extend the theoretical results.
Instillation checking using long short‐term memories for ophthalmology patients
Authors: Ishigami Tomohiro , Isokawa Teijiro , Kamiura Naotake , Masumoto Hiroki , Tabuchi Hitoshi 
Published: December 2022

AbstractIn this paper, we propose a method of checking the eye lotion instillation for ophthalmology patients. Our method first estimates tilt angles of an eye dropper bottle from acceleration values measured by a triaxial sensor attached to the bottle. It next prepares data each of which is equal to a sequence of standardized tilt values, as data to be presented to a discrimination model. It employs either a long short‐term memory (LSTM for short) or a bidirectional long short‐term memory (B_LSTM for short) to construct the model. Once we present the data to be checked to our model, it produces a certainty degree indicating whether a patient corresponding to the presented data applies eye lotion at the time zone in which a sequence of the tilt values used to prepare the presented data was measured. The final judgement for the instillation depends on thresholding of the certainty degree. It is established, from experimental results using practical data, that adopting B_LSTM‐based models is useful in improving metric values compared to adopting LSTM‐based models, and that our models can cope well with the situation specified by the comparatively long time interval of measuring tilt values while some metric values slightly degrade.
Association rule‐based multilevel regression pricing and artificial neural networks based land selling price prediction based on market value
Authors: Krishnasamy Srinivasan , Rajiah Murugasan , SenthilKumar Krishna Kumari , Nagalingam Rajendiran Shanker 
Published: December 2022

SummaryIn Tamil Nadu, India, the guideline value (GV) of land for registration typically corresponds to the market price. After registration of land, Tamil Nadu's land registration office determines which areas have GVs that are lower or higher than the government‐fixed value, and depending on the area. Based on determination of price difference, each are GV is raised or lowered. In other countries expect India, different models, including the hedonic price model, quantile, and multivariate regression are used in the current GV fixation. These models have complex relationships with the many different variables that affect the GV of the land. In this article, novel optimized models for fixing land value according to utilitarian land ethics are proposed, including artificial neural networks (ANN) and associative rule‐based multilevel regression pricing models (AMLP). Increase the GV from the current GV in different percentages using the proposed ANN association rule mining method, and then optimize with spatial and infrastructure parameters from the GIS model builder for real‐time GV generation on the website. For real‐time GV generation on the website, the ANN model uses association rule mining and various neural network architectures. Buyer acceptance test validation of the live GV generation reveals 85% accuracy for the proposed model. The proposed models such as ANN and AMLP, prevent problems with GV fixation and boost revenue for the Indian government.
Optimally combined application of organic and chemical fertilizers increases grain yield and improves rhizosphere microecological properties in rice ratooning
Authors: Lin Manhong , Yang Songwei , Chen Hongfei , Letuma Puleng , Khan Muhammad Umar , Huang Jinwen , Shen Lihua , Lin Wenxiong 
Published: December 2022

AbstractField tests were conducted at experimental station of Chongluo, Nanping city, Fujian province, China in 2018–2019 to explore the action and physiological mechanism of organic nitrogen in rice ratooning under the configurations with different proportions of organic and inorganic nitrogen. The results showed that sole chemical fertilizer (CF) led to a significant decrease in soil nutrient availability, especially at late growth stage, and reduced grain yield of the main and ratooning crops compared with the optimal fertilizer configuration (GM2) in 30% organic nitrogen with 70% chemical nitrogen fertilizer in the premise of total application rate at 225 kg N/ha. Further studies have revealed that GM2 relative to CF treatment promoted to produce more productive panicles of the main crop and had carryover effect on the regeneration rate of ratooning rice due to national C13‐assimilates partitioning in rice–rhizosphere systems, which resulted in increased soil microbial diversity and soil fertility, thereby enhanced yields of the main and ratooning rice crops. These findings help to better understand the action and mechanism of the proper combined application of organic and inorganic nitrogen fertilizer via improving remobilization of 13C assimilates in rice–rhizosphere system, and ultimately increasing grain yield in rice ratooning.
The soursop (Annona muricata): Botany, horticulture, and utilization
Authors: Leal Freddy , Paull Robert E. 
Published: December 2022

AbstractThe South American soursop, guanabana or graviola fruit, was unknown to Europeans until the voyages of Christopher Columbus. The tree is now grown commercially and in home gardens throughout the tropics. The fruit is consumed as a fresh fruit and juice, and the frozen pulp is used in the preparation of ice creams, sherbets, and juices in many world markets. The juice is sometimes mixed with other fruit juices. The pulp is also used in the preparation of drinks, wines, cakes, mousses, gelatins, and puddings or consumed green as a vegetable. This review discusses the taxonomy and morphology of the soursop, cultivar collection, and their use in selection and breeding programs as progenitors for developing new varieties. The environmental requirements, cultural practices, and plant propagation methods for soursop are reviewed. Orchard management that includes planting density, plant nutrition, pest and disease occurrence and control, harvest methods, and yields are discussed. Future prospects are considered.
A comparative study on transmission performance of multi‐stage wireless power transfer systems using SPS compensation and LCC compensation
Authors: Li Jiangui , Wang Chen , Wang Longyang , Ding Yishuo , Yu Tianqi , Cheng Yuying 
Published: December 2022

SummaryThe new multi‐stage wireless power transfer (WPT) systems based on series–parallel–series (SPS) and inductor–capacitor–capacitor (LCC) compensations (SPS‐WPT and LCC‐WPT) are characterized by constant output current and high transmission efficiency. To obtain applicable conditions and performance differences of these two systems, their output characteristics have been studied by theoretical derivation and experimental verification. First, the equivalent models of double‐stage SPS‐WPT and LCC‐WPT systems have been built. Second, comparative analysis of two key compensation components that affect the output performance of the two systems has been completed. Third, the influence of each stage load and coupling conditions on the output performance of the two systems has been further analyzed and compared. Finally, output performance of the two systems has been tested and compared on a newly built experimental platform. Experimental results show that the output power and transmission efficiency of the SPS‐WPT system reach 180 W and 80.39% with offset of 20 mm, which is more suitable for high power applications with low and fixed coupling condition. In addition, the output power and transmission efficiency of the LCC‐WPT system reach 127 W and 85.92% with offset of 10 mm, which is more suitable for high efficiency applications at medium and variable coupling conditions.
Light controls alpine meadow community assembly during succession by affecting species recruitment from the seed bank
Authors: Ma Miaojun , Baskin Carol C. , Zhao Yunpeng , An Hang 
Published: December 2022

AbstractSome research indicates that soil seed banks can promote species coexistence through storage effects. However, the seed bank mechanism that maintains plant assembly and its role in degraded grassland restoration are still not clear. We collected seed bank samples from early, mid and late secondary successional stages of an abandoned subalpine meadow on the Tibetan Plateau, and samples from each stage were exposed to full (i.e., natural), mid, and low light treatments in the field to represent light availability at the bottom/understory (soil surface) of a plant community in the early, mid and late stages of succession, respectively. Species richness, seed density, species composition, and community weighted mean values (CWMs) of seed mass of the species whose seeds germinated in soil samples were evaluated. In response to the light treatments, species richness increased significantly with increased light only for the late successional stage, seed density increased significantly with increased light only in the early and mid successional stages, and seed mass decreased significantly with increased light only in the mid and late successional stages. Species composition differed significantly among the light treatments only in the late successional stage. For the successional series, species richness and seed mass of the species that germinated increased significantly with succession only under mid and full light treatments. Seed density decreased significantly with succession in each light treatment. Species composition differed significantly between the early‐ and late stage and between the mid and late stage in each light treatment. Both the abiotic (light) and biotic (seed mass) factors influence seed bank recruitment to the plant community. Regeneration of small‐seeded species in the seed bank was inhibited under low light in the late successional stage. The balance of stochastic and deterministic processes along a successional gradient was determined by regeneration from the seed bank depending on light intensity change. Differences in seed response to light intensity change largely determined plant community assembly. Our findings should help in the development of effective conservation and restoration strategies.