bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral hygiene behaviours and their associated factors in Chinese adolescents: Evidence from the National Oral Health Survey
Authors: Wang Wenhui , Cui Zhiying , Du Shuo , Yu Yizhen , Xu Kaige , Wang Mingyue , Wang Xing , Feng Xiping , Tai Baojun , Hu Deyu , Lin Huancai , Wang Bo , Wang Chunxiao , Zheng Shuguo , Liu Xuenan , Rong Wensheng , Wang Weijian , Si Yan 
Published: November 2022

Abstract
Objectives To investigate oral hygiene behaviours and their correlates among Chinese adolescents.
Materials and Methods This cross-sectional study recruited 118,601 subjects aged 12-15 years from the National Oral Health Survey using multistage cluster sampling. Questionnaire data were used for descriptive and multivariate analyses.
Results A total of 32.6% of subjects brushed their teeth twice daily, 7.4% used fluoridated toothpaste, 3.9% cleaned their teeth twice daily with fluoridated toothpaste, and 9.7% used dental floss. Multivariable logistic regression showed that the probability of good oral hygiene practices (twice-a-day tooth brushing with fluoridated toothpaste) was higher among these groups: females (AOR: 1.141; 95%CI: 1.072-1.214), 15-year-olds (AOR: 1.786; 95%CI: 1.634-1.952), from urban areas (AOR: 1.389; 95%CI: 1.288-1.497), without siblings (AOR: 1.351; 95%CI: 1.259-1.450), with an educated father (AOR: 1. 605; 95%CI: 1.442-1.788) and mother (AOR: 1.706; 95%CI: 1.530-1.903), having dental visit experiences (AOR: 1.702; 95%CI: 1.589-1.823), rating one’s oral health as good (AOR: 2.341; 95%CI: 2.083-2.631), having fluoride knowledge (AOR: 4.345; 95%CI: 4.034-4.678) and having a positive attitude towards regular dental care (AOR: 1.589; 95%CI: 1.460-1.729).
Conclusion The oral hygiene behaviours of Chinese adolescents were undesirable. Good oral hygiene practices are significantly associated with sociodemographic factors, fluoride knowledge, and attitudes towards regular dental care.
Clinical Relevance Professionals, parents and adolescents should pay attention to adolescents' oral hygiene behaviours. It should be emphasized that twice-a-day tooth brushing with fluoridated toothpaste is essential when designing the content of oral health education and when counselling adolescents.
A Anoikis-Related Risk Model: Predicts Prognosis and Immunotherapy Response for Hepatocellular Carcinoma
Authors: Ren Haitao , Kang Na , Yin Shuan , Xu Chen , Qu Tengfei , Dai Dongdong 
Published: November 2022

Abstract
Hepatocellular Carcinoma (HCC) is the leading cause of cancer-related deaths globally. Most HCC patients are already in advanced stages of the disease when a confirmed diagnosis was made with prone to metastasis and a poor prognosis. Anoikis resistance plays a critical role in tumor invasion and metastasis. whereas the role of anoikis in HCC remains unclear. According to univariate Cox regression and the least absolute shrinkage and selection operator (LASSO) analysis, anoikis-related genes (ARGs) associated with the overall rate (OS) were selected. Then, 3 prognostic ARGs (PDK4, STK11 and TFDP1) were identified by multivariate Cox regression, and to establish a risk model. According to the risk score, HCC patients were divided into high- and low-risk group. The OS rate and immune infiltration between two groups were evaluated by Kaplan-Meier, CIBERSORT and ssGSEA analysis. The OS rate of HCC patients in low-risk group was longer than that in the high-risk group. The results of nomogram showed that the ARGs prognostic signature was an independent prognostic predictor. In addition, consensus clustering analysis could cluster the patients into two subgroups with different immune infiltration. Besides, functional enrichment and drug sensitivity were also conducted between high- and low-risk groups. This study was the first to integrate multiple ARGs to establish a risk-predictive model, and might provide a new perspective for individualized and accurate therapy strategies for HCC patients.
The Coexistence of Pituitary Adenoma and Rathke’s Cleft Cyst with Situs Inversus Totalis: A case presentation and review of literature
Authors: Zhao Guohong , Heng Lijun , Li Junting , Wang Hang , Zhang Nanyan , Yu Xinwen , Gao Bin 
Published: November 2022

Abstract
Background

Situs inversus totalis (SIT) is a rare anatomical condition affecting the positioning of internal organs. Rathke’s cleft cyst (RCC) and pituitary adenoma (PA) are the most common brain diseases, but the coexistence of PA and RCC is extremely rare. To our knowledge, SIT with PA and RCC has never been reported.

Case presentation

We retrospectively analyzed a case of SIT with Cushing's disease and RCC in a 65-year-old woman. The main complaints were centripetal obesity and limb weakness for two months. Heart ultrasonography (USG), chest, and abdominal CT revealed a mirror image of internal organs. In view of the elevated cortisol level, the cortisol suppression tests were applied to confirm the diagnosis of Cushing’s disease. Magnetic resonance imaging (MRI) demonstrated a pituitary microadenoma and a cystic lesion (RCC). Endoscopic endonasal surgery was done and the final pathology confirmed both lesionsascorticotroph adenoma and RCC respectively. However, whole-exome sequencing (WES), including mitochondria, did not return a positive result for adrenocorticotropic hormone (ACTH). At the three months post-operative follow-up, the patient’s weight had decreased by 3.5 kg and symptoms were significantly reduced. PA with co-occurrence of RCC is very rare. Of the patients who underwent PA surgery at the Tangdu hospital from 2015 to 2020, only five cases were reported to also have RCC (0.5%).

Conclusions

In this single-case study, we describe a woman with confirmed SIT who also has manifestations of hypercortisolism, which was eventually confirmed to be PA combined with RCC. In view of the possibility of a gene mutation leading to SIT during embryonic development, and since RCC and PA are also considered to occur in this period, although this patient did not find any abnormal genetic variations, we still can't rule out that the abnormality in this case is caused by abnormal development of early embryos.
Pharmacoinformatics-based prediction of potential Keap1 inhibitors from Hemidesmus indicus (L) R.Br. against oxidative stress-induced diseases
Authors: Vellur Senthilkumar , Pavadai Parasurman , Palanichamy Chandrasekar , Kabilan Shanmugampillai Jeyaraguru , Pandian Sureshbabu Ram Kumar , Kunjiappan Selvaraj 
Published: November 2022

Abstract
The Keap1-Nrf2 pathway plays a prominent role in activating cytoprotective genes, detoxification and antioxidative defense enzymes against oxidative stress and xenobiotics-induced damage. Oxidative stress is involved in the initiation and progression of numerous health complications. The present study investigated the antioxidant potential of aqueous methanolic extract of Hemidesmus indicus (L) R.Br., followed by a pharmacoinformatics-based screening of novel Keap1 protein inhibitors. Initially, the antioxidant potential of this target plant was assessed by antioxidant assays (DPPH, ABTS radical scavenging and FRAP). H. indicus (L) R.Br. extract (100 µg mL− 1) showed 85 ± 2.917%, 78.783 ± 0.24% of DPPH, ABTS radicals scavenging activity, and 161 ± 4 µg mol (Fe (II)) g− 1 ferric ion reducing power. Further, a total of sixty-nine phytocompounds were derived from this plant through the IMPPAT database, and their three-dimensional structures were obtained from the PubChem database. The chosen sixty-nine phytocompounds were docked against the Kelch-Neh2 complex protein (PDB entry ID: 2flu, resolution 1.50 Å) along with the standard drug (CPU192018). The top scored three hits were selected, namely Hemidescine (− 11.30 Kcal mol− 1), Beta-Amyrin (− 10.00 Kcal mol− 1), and Quercetin (− 9.80 Kcal mol− 1) based on their binding affinities. The selected three hits showed significant drug-likeness properties with the least toxicity profile. Molecular dynamics simulation studies showed that all the protein-ligand complexes (Keap1-HEM, Keap1-BET and Keap1-QUE) were highly stable during the entire simulation period, compared to standard CPUY192018-Keap1complex. Based on these findings, the top-scored three phytocompounds may be used as a significant and safe Keap1 inhibitor and could potentially use for oxidative stress-induced health complications.
Deep Belief Structure Learning for Finger Vein Verification by Solving the Biometric Recognition Model
Authors: Muthusamy Dharmalingam , Ponnusamy Rakkimuthu 
Published: November 2022

Abstract
Vein authentication is a novel biometric method to authenticate the individuality of a person. The conventional biometric technique employs shape images and exact segments of finger veins for the verification process. To improve the verification accuracy, a novel Anisotropic Filtered Stromberg Feature Transform based on Tucker’s Congruence Deep Belief Structure Learning (AFSFT-TCDBSL) technique is intended. The proposed AFSFT-TCDBSL technique comprises one input, three hidden, and one output layers. The numbers of images are collected in the input, and input images are preprocessed using anisotropic diffusion filtering in the first hidden layer. Finally, the verification process is performed using Tucker’s congruence correlation coefficient (TCCC). Based on the correlation, the verification outputs are getting to the output layer. In this way, accurate finger vein verification is performed with superior accuracy and with a minimum false rate. We performed experimental assessments with different factors, such as PSNR, FVVA, FPR, and CT. The proposed ADFSFT-TCDBSL technique offers better finger-vein verification results than the state-of-the-art methods.
Exploration on Challenges which Hinder Sexuality Education in Public Secondary Day Schools in Tanzania
Authors: Mboneko Ntacho Edina , Iramba Iramba Freddie 
Published: November 2022

Abstract
The purpose of this paper was to explore the challenges which hinder sexuality education in public day secondary schools in Tanzania. The study was conducted in Geita region and covered three secondary schools of Geita district. Phenomenological research design with purposive and non-random sampling was used to select obtain 30 informants, 24 students from form one to form four and 6 guidance and counselling participant teachers. The study employed semi-structure interview to teachers and focus group discussion to students at capturing views on the challenges hinder effective implantation of comprehensive sexuality education in secondary day schools. Data which was collected were analyzed thematically. The findings revealed that sexuality education challenges which were identified were those related to economic, cultural, institutional, globalization, and teachers’ knowledge. Economic factors included family poverty and lack of students’ basic needs. Cultural factors include those from religious beliefs and taboos which prohibit talking sexuality issues to young people while those related to institution include limited sexuality time and unfavorable environment. Those challenges related to globalization included social Medias and high exposure to phonographs from the internet. Therefore, it was discovered that uncontrolled economic, cultural, institutional, social Medias and teachers’ training related challenges led adolescent students to engage in risky sex behaviors and experience its side effects including early pregnancies.
Physical and Mechanical Properties of Papers Produced From Different Cellulosic Pulps Blending
Authors: Pego¹ Matheus Felipe Freire , Bianchi² Maria Lúcia , Yasumura³ Patrícia Kaji 
Published: November 2022

AbstractThis study aimed to evaluate the effect of different fiber blending in the physical-mechanical properties of papers and understand to what extent the fiber blending influence produced paper quality. Three different commercial cellulosic pulps were used: eucalyptus, sisal, and pine pulp. Fiber morphological analyses were performed after refining in each pulp. The pulps were blended two by two in 5/95%, 25/75%, and 45/55% ratio in all possible combinations. Handsheets were formed (2% consistency) in a lab papermaking machine and tested by physical and mechanical properties. Virgin pulps (without blending) were also used for handsheet production. Fibers presented different features regarded to morphological properties and indexes. Most significant differences were related to fiber length. Statistical differences occurred in all physical and mechanical properties. Differences were due to morphological features. The highest and lowest values were pointed out for each property. Thickness tended to decrease with fiber blending in all proportion. Thickness and grammage were not related. For all mechanical properties, the lowest values were obtained in eucalyptus treatment and blending involving it. The highest values were obtained in pine, sisal, and blending treatments. A small addition of sisal (5%) in eucalyptus pulp improved the tensile strength, tensile index, stretch, bursting index, tear index, and fold endurance in approximately 41.5, 54.8, 51.4, 28.9, 37.5, and 33.3%, respectively. The same addition using pine resulted in an improvement of 15.9, 22.7, 22.7, 37.4, 46.7, and 133.3%. Fiber blending presented a synergetic effect for physical and mechanical properties.
Proteomic landscape of primary and metastatic brain tumors for heterogeneity discovery
Authors: Yang Shuang , Zhou Chengbin , Zhang Lei , Xiong Yueting , Zheng Yongtao , Bian Liuguan , Liu Xiaohui 
Published: November 2022

Abstract
Background
Brain tumors, whether primary or secondary, have limited information about proteomic changes despite advances in the understanding of the driver gene mutations and heterogeneity within tumor cells. In this study, we for the first time systematically analyzed the proteome of tumor tissues from patients with brain metastases (BrMs) and gliomas.
Methods
We assembled the most common primary tumors as follows: gliomas from WHO grade II to IV with or without IDH1 mutations; and BrMs with a wide range, including lung cancer (L.C), breast cancer (B.C), ovarian cancer (O.C), and colorectal cancer (C.C). A total of 29 tissue samples were analyzed by label free quantitative mass spectrometry-based proteomics.
Results
In total, 8,370 protein groups were identified, and approximately 4,000 quantified protein groups were adopted for further analysis. Proteomic analysis of metastatic tumors reveals conserved features across multiple cancers. While proteomic heterogeneities were found for discriminating low- and high-grade of gliomas, as well as IDH1 mutant and wild-type gliomas. And distinct pathway-level differences among these two types of brain malignancies were revealed. The characteristic pathways of BrMs focused on proliferation and immunomodulation after colonizing the brain, whereas invasion processes were notably activated in gliomas.
Conclusion
We elucidated an extensive proteomic landscape of BrMs and gliomas, providing information for the development of potential therapeutic and diagnostic strategies for type-specific brain tumors.
Enolase inhibition reduces autophagy and promotes neurological function recovery after spinal cord injury
Authors: Zhou Zhiqiang , Wu Jie , Yang Xiaohui , Pan Wenming , Li Xigong , Zhang Qin 
Published: November 2022

Abstract
Spinal cord injury (SCI) is a highly debilitating condition that impairs neuronal function and triggers a complex cascade of primary and secondary damage events. Autophagy can result in cell death, which is a phenomenon in eukaryotic cells and plays an important role in the development of SCI. Thus, this provides a practical and potential target for therapeutic intervention against SCI. Enolase inhibition (ENOblock) has been demonstrated to provide neuroprotection and functional recovery in a rat model of SCI. However, it remains unclear whether ENOblock can mediate neuronal autophagy after SCI. For rat model of SCI, All rats underwent laminectomy from T8 to T10. Sci rats were subjected to dorsal spinal cord exposure and SCI was injured using a modified Allen’s weight-drop apparatus. Locomotor function was assessed using the Basso, Beattie, and Bresnahan(BBB) rating scores. Thereafter we investigated the expression levels of autophagy-related proteins and Enolase by by western blot, immunofluorescence and quantitative polymerase chain reaction analysis. We found that the expression levels of autophagy-related proteins (LC3-Ⅱ and Beclin-1) and Enolase were significantly increased after acute SCI. In contrast, ENOblock could down-regulate the expression of these proteins and improve motor function after SCI. The difference between the SCI group and ENOblock group was most pronounced on the third and seventh day. Based on the above mentioned data, we speculate that ENOblock may play a role in inhibiting autophagy activity and promoting functional recovery after SCI in rats.
Landowner preferences pave the way for the incorporation of tree configurations on private land in rural Costa Rica
Authors: Lehnert Savannah L. , Grimm Kerry E. , Aslan Clare E. , Frey Sarah J.K. , Mark Melissa M. 
Published: November 2022

Abstract
Agroecology is gaining increasing attention in tropical regions as a solution to maintaining robust ecosystem services for rural communities and down-stream users. The incorporation of tree configurations into working landscapes is a specific agroecology practice, that to be effective, must be driven by regional values and preferences. In rural Costa Rica, we used a photo-based survey to determine landowner preferences for tree configurations that could be incorporated into landscape design on private property. We examined landowner motivations for having trees on private land, as well as community-identified barriers to maintaining tree cover. We found that landowners were very interested in the incorporation of large tree configurations (e.g., wide riparian buffer zones; large, forested patches) and that preferences did not vary significantly by demographics (respondent’s property size; income source). Respondents identified various regulating, supporting, and cultural ecosystem services as important motivating factors for maintaining trees on private land, whereas provisioning services and legal reasons were not as strong motivating factors. While preferred tree configurations supported the values that landowners identified as important (e.g., habitat provisioning), barriers may inhibit the actual implementation of such configurations, including limited space and financial resources. To overcome such barriers, results indicate that a shift may be needed within Costa Rica’s Payment for Ecosystem Service program to increase participation among small landholders interested in tree conservation. Increasing dialog with local communities can generate a deeper understanding of potential challenges and landowner interest in tree incorporation, which can then inform regional agroecology recommendations and policy.
Drought legacy regulates the stability of greenhouse gases emissions of aquatic sediments
Authors: Bonhomme Camille , Leroy Céline , Céréghino Régis , Marinho Claudio C. , Figueiredo-Barros Marcos Paulo , Farjalla Vinicius 
Published: November 2022

Abstract
Although climate change models predict more frequent and severe climatic events such as droughts, the effects of drought on greenhouse gas (GHG) emissions from lentic ecosystems are still poorly understood. Moreover, little is known of the extent to which drought effects on GHG emissions depends on the occurrence and intensity of previous droughts (legacy effect). Here, we examined the temporal stability of methane (CH4) and carbon dioxide (CO2) emissions during rewetting after a drought, and how drought legacy modulates this response. Laboratory microcosms containing sediments from natural lentic ecosystems were pre-exposed to drying-rewetting treatments (hereafter “D-RW event”) that differed by the durations of both the drying and rewetting periods. Then, we analyzed the resistance and the recovery rates of CH4 and CO2 emissions following a second drying-rewetting event equally established in all treatments. Water column was kept constant in controls. We found a pulse of CH4 and CO2 emissions upon the second dry-rewetting event, followed by a progressive recovery toward control emissions. This response was regulated by the drought legacy effect: the recovery of GHG emissions was faster in microcosms that had been subjected to a previous drought. For CO2 emissions, the drought legacy effect was stronger (faster recovery) where previous drought was longer. We therefore emphasize the incorporation of GHG emissions during D-RW events into GHG emission estimates, especially because climate models predict scenarios of drought intensification. While contemporary climate should be the dominant driver of GHGs from lentic ecosystems, previous drought can also regulate their response to D-RW events.
PacBio Full-length Transcriptome Analysis Provides New Insights into Transcription of Chloroplast Genomes
Authors: Gao Shan , Shi jinsong , Yan Shuangyong , Li Wenjing , Yang Xiurong , Cui Zhongqiu , Li Junling , Li Guangsheng , Li Yuejiao , Hu Yanping 
Published: November 2022

Abstract
Background: Chloroplast and mitochondrial DNA (cpDNA and mtDNA) are apart from nuclear DNA (nuDNA) in a eukaryotic cell. The transcription system of chloroplasts is different from those of mitochondria and eukaryotes. In contrast to mtDNA and nuDNA, the transcription of cpDNA remains unclear, mainly because the identification of the cp transcription initiation sites (TISs) and transcription termination sites (TTSs)remains unresolved.
Results: In the present study, we characterized the transcription of cp genes with more comprehensive and accurate information using PacBio full-length transcriptome data of Arabidopsis thaliana. The main results included the discovery of four types of artifacts, the validation and correction of cp gene annotations, the discovery of polyA-like sites as TTSs and the exact identification of “GA”- and “GT”-type TISs on the genome scale. Notably, we proposed a new model to explain how the cptranscription initiates and terminates at the genome level.
Conclusions: Four types of artifacts, degraded RNAs and splicing intermediates deserve the attention of every scientist who is working with PacBio full-length transcriptome data, as these contamination sequences could mislead the downstream analysis. The cp transcription initiates at multiple promoters and terminates at polyA-like sites. Our study provides new insights into the cp transcription and new clues to reveal the origins of promoters, TISs, TTSs and polyA tails of eukaryotic genes.