bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular detection of a novel Ancylostoma sp. by whole mtDNA sequence from pangolin Manis javanica
Authors: Tuli Merga Daba , Li Hongyi , Li Song , Zhai Junqiong , Wu Yajiang , Huang Wanyi , Feng Yaoyu , Chen Wu , Yuan Dongjuan 
Published: March 2022

Abstract
Background
Ancylostoma species are hematophagous parasites that cause chronic hemorrhage in various animals and humans. Pangolins, also known as scaly anteaters, are mammals that live in soil environments where they are readily exposed to soil-borne parasitic nematodes. However, only a limited number of helminth species have been identified in this animal host so far.

Methods
Ancylostoma sp. was isolated from a wild pangolin, and the complete mitochondrial (mt) genome of Ancylostoma sp. was obtained by Illumina sequencing of total genomic DNA.

Results
The circular complete mt genome that was assembled had a total length of 13,757 bp and comprised 12 protein-coding genes (PCGs), 22 transfer ribosomal RNAs, two ribosomal RNAs (rRNAs), two non-coding regions and one AT-rich region, but lacked the gene coding for ATPase subunit 8 (atp8). The overall AT content of the mt genome of Ancylostoma sp. was 76%, which is similar to that of other nematodes. The PCGs used two start codons (ATT and TTG) and three stop codons (TAA, TAG, and T). The nucleotide identity of the 12 PCGs ranged from 83.1% to 89.7% and had the highest sequence identity with Ancylostoma caninum among species in the Ancylostomatidae family. Also, the pangolin-derived Ancylostoma sp. lacked repeat sequences in the non-coding regions and in the unique sequence of the short non-coding regions, which differentiated it from other Ancylostoma species. In addition, phylogenetic analyses of 18S rRNA and mtDNA sequences revealed that the Ancylostoma sp. was positioned in a separate branch in the subfamily Ancylostomatinae along with other Ancylostoma species.

Conclusions
The Ancylostoma sp. isolated from a pangolin in this study was identified as a possible new Ancylostoma species. The identification of this Ancylostoma sp. from pangolin enriches our knowledge of the species in the Ancylostomatidae family and provides information that will lead to a better understanding of the taxonomy, diagnostics, and biology of hookworms.

Graphical Abstract
RNA profiling of blood platelets noninvasively differentiates colorectal cancer from healthy donors and noncancerous intestinal diseases: a retrospective cohort study
Authors: Xu Luming , Li Xinbo , Li Xiangchun , Wang Xingyue , Ma Qian , She Dan , Lu Xiaohuan , Zhang Jiao , Yang Qianqian , Lei Shijun , Wang Lin , Wang Zheng 
Published: March 2022

Abstract
Background
The RNA profiles of tumor-educated platelets (TEPs) possess pathological features that could be used for early cancer detection. However, the utility of TEP RNA profiling in detecting early colorectal cancer (CRC) versus noncancerous colorectal diseases has not yet been investigated. This study assesses the diagnostic capacity of TEP RNA profiles in a cohort of patients with CRC and noncancerous diseases.

Methods
Transcriptome sequencing for platelets isolated from 132 patients with CRC at early and late stages and 190 controls consisting of healthy donors and patients with ulcerative disease, Crohn’s disease, polyps, and adenomas was performed and analyzed using binary particle swarm optimization coupled with support vector machine to identify genes that contributed to the classification of CRC patients versus controls. The area under the receiver operating curves (AUROCs) and the accuracy of TEP RNA profiles in CRC diagnosis were assessed.

Results
TEP RNA profiling achieved high performance in distinguishing and staging CRC patients from the controls. Using the swarm intelligence algorithm, the 921 most contributive genes that classified CRC patients from the controls were identified. AUROCs of 0.928 for the training set via leave-one-out cross-validation and 0.92 for the validation set were achieved, both of which were significantly higher than the clinically utilized serum biomarkers: carcinoembryonic antigen and cancer antigen 19-9. Notably, an AUROC of 0.915 in an external validation set was achieved. For predicting different CRC stages, an AUROC of 0.984 was achieved in the training set and 1.000 in the internal validation set.

Conclusions
RNA profiles of TEPs are of potential diagnostic value for identifying early CRC from noncancerous diseases. Prospective studies are needed to validate its clinical relevance.
Rhizophagus intraradices improves arsenic tolerance in Sophora viciifolia Hance
Authors: Zhang Qiaoming , Gong Minggui , Xu Shanshan , Wei Yanan , Yuan Jiangfeng , Chang Qingshan 
Published: March 2022

Abstract
Purpose
Arbuscular mycorrhizal fungi (AMF) are capable of protecting host plants against heavy metal toxicity, whereas rare knowledge has been acquired on the underlying effects of AMF on woody leguminous species under arsenic (As) stress. This study was aimed that whether AMF inoculation improves the As tolerance in Sophora viciifolia (a king of leguminous shrub).

Methods
S. viciifolia seedlings were inoculated with AMF Rhizophagus intraradices, and then the seedlings were grown at three levels (0, 50, and 100 mg As kg−1 soil) of As-polluted soil by performing the potted experiments. The plant growth, photosynthetic parameter, oxidative damage, antioxidant enzyme activities and gene expression of phytochelatin synthase in R. intraradices- and non-inoculated S. davidii seedlings under the different levels of As stress were analyzed.

Result
Compared with non-inoculated S. viciifolia at the identical As level, R. intraradices-inoculated S. viciifolia were higher in the shoot and root dry weight, plant height, root length, photosynthetic rate, stomatal conductance, transpiration rate, maximal photochemical efficiency of PSII photochemistry, actual quantum yield, and photochemical quenching values, while the intercellular CO2 concentration and non-photochemical quenching values were lower. As-induced oxidative stress generating malondialdehyde, hydrogen peroxide and superoxide in the S. viciifolia leaves and roots reduced significantly by R. intraradices inoculation, whereas the activities of antioxidative enzymes (e.g., superoxide dismutase, peroxidase, and catalase) in S. viciifolia leaves and roots were increased by R. intraradices inoculation. Notably, R. intraradices inoculation up-regulated the gene expression of S. viciifolia phytochelatins in the leaves and roots.

Conclusion
These results demonstrated that R. intraradices inoculation enhanced the As tolerance in S. viciifolia seedlings by improving the plant growth, gas exchange, chlorophyll fluorescence, reactive oxygen species, antioxidant enzymes and gene expression of S. viciifolia phytochelatins. The present study verified a multifarious positive role of AMF for woody leguminous species under As stress.
Downlink transmission and channel estimation for cell-free massive MIMO-OFDM with DSDs
Authors: Guo Yunxiang , Fan Zhenqi , Lu An , Wang Pan , Liu Dongjie , Xia Xinjiang , Wang Dongming 
Published: March 2022

AbstractIn a cell-free massive MIMO system, multiple users arrive at multiple access points at separate times, while in an OFDM system, different delays can be equivalent to delay spread differences (DSDs). Since DSDs are not all the same, in the downlink transmission process, it is necessary to consider its impact on transmission techniques, such as channel estimation and downlink precoding. In this paper, aiming at the performance loss caused by DSDs in cell-free massive MIMO-OFDM system, we propose a multi-RB precoding optimization algorithm that maximizes the downlink sum rate. We derive the sum rate maximization problem into an iterative second-order cone programming form to achieve convex approximation. Then, considering the impact of DSDs on the accuracy of cell-free massive MIMO-OFDM channel estimation, we propose a downlink channel estimation method, which jointly uses channel state information reference signal and demodulation reference signal. The simulation results show that the proposed multi-RB optimal precoding can effectively improve the downlink sum rate, and the proposed downlink channel estimation can obtain accurate multi-RB frequency-domain channel parameters.
Blockchain localization spoofing detection based on fuzzy AHP in IoT systems
Authors: Lv Wenzhe , Qiu Xuesong , Meng Luoming 
Published: March 2022

AbstractLocation spoof detection is a major component of location proofing mechanisms in internet of things (IoT), and it is significant for the system to assess the trustworthiness of the location data associated with the user. Unlike the work that employs physical layer features, we interest in building the infrastructure for a solution to establish location spoofing detection capabilities in blockchain-based IoT systems. In detail, at the node and the mobile trajectory level, we create an IoT system for evaluating the trustworthiness of location proofs with blockchain location system features. A blockchain-based multilayer fuzzy hierarchical analysis process (AHP) evaluation method is contemplated to detect location spoofing in the IoT system. Simulation results indicate the proposed method has a superior performance and provides a basis for the trustworthiness assessment of location proofs.
A new iterative approximation scheme for Reich–Suzuki-type nonexpansive operators with an application
Authors: Ofem Austine Efut , Işık Hüseyin , Ali Faeem , Ahmad Junaid 
Published: March 2022

AbstractIn this article, we propose a faster iterative scheme, called the AH iterative scheme, for approximating fixed points of contractive-like mappings and Reich–Suzuki-type nonexpansive mappings. We show that the AH iterative scheme converges faster than a number of existing iterative schemes for contractive-like mappings. The $w^{2}$

w
2

-stability result of the new iterative scheme is established and a supportive example is provided to illustrate the notion of $w^{2}$

w
2

-stability. Then, we prove weak and several strong convergence results of our new iterative scheme for fixed points of Reich–Suzuki-type nonexpansive mappings. Further, we carry out a numerical experiment to illustrate the efficiency of our new iterative scheme. As an application, we use our main result to prove the existence of a solution of a mixed-type nonlinear integral equation. An illustrative example to validate the result in our application is also given. Our results extend and generalize several related results in the existing literature.
Resistant starch (RS), a novel endogenous inert marker for detecting glucose absorption of small intestine with sweeteners administration in mice
Authors: Wu Yaran , Cai Lei , Xie Xingzi , Yang Shuying , Shi Qing , Jia Hongzhe , Gu Xuqiang , Deng Jingmin , Shi Mingzhao , Chen Qiuping , Cao Shaoqian , Cai Shuangfeng 
Published: March 2022

AbstractResistant starch could be degraded by the fermentation of colonic microorganisms in the large intestine of mammals, but not in the small intestine. In this study, we established a novel strategy by using resistant starch as an endogenous marker to determine the glucose absorption of the small intestine of laboratory animals. By optimization of the classical enzymatic method of starch measurement, the demand for the sample weight was reduced by 90%. Moreover, the amount of resistant starch in normal feed was detectable without any extra addition. The value of small intestine glucose absorption of mice was similar when using resistant starch and titanium dioxide as inert markers. The fermentation of resistant starch by intestinal microorganisms in the small intestine was demonstrated not disturbing the detection of glucose absorption significantly. Artificial sweeteners exposed ICR mice showed different glucose absorption which indicated, first, resistant starch can be used as a novel endogenous marker in the small intestine of small animals; second, although glucose tolerance did not change in mice after short-term exposure to artificial sweeteners, there were significant changes in glucose absorption associated with it; third, the short-term exposure resulted in no significant change in glucose tolerance.
Buprenorphine/naloxone access for people with opioid use disorder in correctional facilities: taking steps to support knowledge translation
Authors: Regenstreif Lori , Sadik Marina , Beaulieu Erin , Bodkin Claire , Kiefer Lori , Guenter Dale , Lee Patsy W. P. , Kouyoumdjian Fiona G. 
Published: March 2022

AbstractPeople with opioid use disorders are overrepresented in correctional facilities, and are at high risk of opioid overdose. Despite the fact that buprenorphine/naloxone is the first line treatment for people with opioid use disorder, there are often institutional, clinical, and logistical barriers to buprenorphine/naloxone initiation in correctional facilities. Guided by the knowledge-to-action framework, this knowledge translation project focused on synthesizing knowledge and developing a tool for buprenorphine/naloxone initiation that was tailored to correctional facilities, including jails. This information and tool can be used to support buprenorphine/naloxone access for people in correctional facilities, in parallel with other efforts to address barriers to treatment initiation in correctional facilities.
Mental health, learning behaviour and perceived fatigue among university students during the COVID-19 outbreak: a cross-sectional multicentric study in the UAE
Authors: Mosleh Sultan M. , Shudifat Raed M. , Dalky Heyam F. , Almalik Mona M. , Alnajar Malek K. 
Published: March 2022

Abstract
Background
The rapid shift to online education due to COVID-19 quarantine challenged students’ ability to accept pure online learning without negative consequences for their physical, emotional and mental health. Some educational institutions introduced new strategies to reduce the psychosocial burden associated with online learning during home confinement. Thus, the primary aims were to determine the consequences of COVID-19 for the psychological well-being and fatigue levels of higher education students and to explore the effects of a new academic assessment approach in reducing home confinement stress.

Method
A cross-sectional online survey was conducted among students, from 30 August to 30 September 2020, of 7 disciplines in all 16 higher colleges of technology in the United Arab Emirates (UAE). The Mental Well-being and Learning Behaviours Scale and the modified Copenhagen Burnout Inventory were used to evaluate students’ psychological well-being and fatigue levels. A Welch t-test and Welch ANOVA were performed to determine the differences in perceived psychological well-being associated with students’ characteristics. Second, Kruskal_Wallis and Mann_Whitney were performed to determine the differences in fatigue level based on students’ characteristics.

Results
One thousand four hundred students participated. The majority were female (78.5%) and aged from 21–25 years (58.1%). Around 14% of respondents were married with children. Nearly 40% were satisfied with the new assessment approach introduced during the COVID pandemic and 45.5% perceived it as having reduced their home confinement stress. The mean psychological distress score of 3.00 (SD ± 0.71) indicates a moderate impact of COVID-19 on psychological well-being. Students’ psychological distress was positively correlated with fatigue level (0.256, p < 0.001) and negatively correlated with the perceived impact of the new assessment approach on student lifestyle (− 0.133, p < 0.001), physical health (− 0.149, p < 0.001) and coping with stress (− 0.125, p < 0.001). Male students experienced significantly lower fatigue and better psychological well-being than female students.

Conclusion
The study reveals that new assessment approaches which emerged during home confinement reduced students’ perception of stress and of impaired lifestyle. However, students still had a considerable burden of psychological distress, requiring further preventive measures to maintain their psychological well-being during future outbreak events. Educational institutions should consider additional strategies to improve students’ preparedness for online teaching, which could help maintain their psychological well-being.
Alternative migratory strategies related to life history differences in the Walleye (Sander vitreus)
Authors: McKee Graydon , Hornsby Rachael L. , Fischer Friedrich , Dunlop Erin S. , Mackereth Robert , Pratt Thomas C. , Rennie Michael 
Published: March 2022

AbstractBackgroundWhile Pace of Life Syndrome predicts behavioural differences between individuals with differential growth and survival, testing these predictions in nature is challenging due to difficulties with measuring individual behaviour in the field. However, recent advances in acoustic telemetry technology have facilitated measurements of individual behaviour at scales not previously possible in aquatic ecosystems.MethodsUsing a Walleye (Sander vitreus) population inhabiting Black Bay, Lake Superior, we examine whether life history characteristics differ between more and less mobile individuals as predicted by Pace of Life Syndrome. We tracked the movement of 192 individuals from 2016 to 2019 using an acoustic telemetry study, relating patterns in annual migratory behaviour to individual growth, and seasonal changes in optimal thermal-optical habitat.ResultsWe observed two consistent movement patterns in our study population—migratory individuals left Black Bay during late summer to early fall before returning to the bay, whereas residents remained within the bay year-round. The average maximum length of migrant Walleye was 5.5 cm longer than residents, and the sex ratios of Walleye caught during fall surveys was increasingly female-biased towards the mouth of Black Bay, suggesting that a majority of migrants were females. Further, Walleye occupancy outside of Black Bay was positively associated with increasing thermal-optical habitat.ConclusionsWalleye in Black Bay appear to conform to Pace of Life Syndrome, with migrant individuals gaining increased fitness through increased maximum size, which, given size-dependent fecundity in this species, likely results in greater reproductive success (via greater egg deposition vs. non-migrants). Further, apparent environmental (thermal) controls on migration suggest that migratory Walleye (more so than residents) may be more sensitive to changing environmental conditions (e.g., warming climate) than residents.
Management of COVID-19 vaccines cold chain logistics: a scoping review
Authors: Fahrni Mathumalar Loganathan , Ismail Intan An-Nisaa’ , Refi Dalia Mohammed , Almeman Ahmad , Yaakob Norliana Che , Saman Kamaliah Md , Mansor Nur Farhani , Noordin Noorasmah , Babar Zaheer-Ud-Din 
Published: March 2022

Abstract
Background
Successful mass vaccination programmes are public health achievements of the contemporary world. While pharmaceutical companies are actively developing new vaccines, and demonstrating results of effectiveness and safety profiles, concerns on COVID-19 vaccine management are under-reported. We aimed to synthesise the evidence for efficient cold chain management of COVID vaccines.

Methods
The scoping review’s conduct and reporting were based on the PRISMA–ScR 2018 checklist. We searched from April 2020 to January 2022 for publications in PubMed (LitCovid), Scopus and ScienceDirect. All review stages were pilot-tested to calibrate 2 reviewers. Articles on cold chain logistics and management were included, while publications solely describing COVID vaccines, their development and clinical aspects of the vaccine, were excluded. To capture relevant data, charting was conducted by one reviewer and verified by another. Results were analysed thematically and summarised descriptively in a table and in-text.

Results and discussion
We assessed 6984 potentially relevant citations. We included 14 publications originating from USA (n = 6), India (n = 2), Finland, Spain, Bangladesh, Netherlands, Switzerland and Ethiopia. They were reported as reviews (4), policy or guidance documents (3), experimental studies (2), case reports (2), expert commentary (1), phenomenological study (1), and decision-making trial and evaluation laboratory trial (1). The findings were presented in three themes: (i) regulatory requirements for cold-chain logistics, (ii) packaging and storage, and (iii) transportation and distribution. A conceptual framework emerged linking regulatory requirements, optimal logistics operation and formulation stability as the key to efficient cold chain management. Recommendations were made for improving formulation stability, end-product storage conditions, and incorporating monitoring technologies.

Conclusion
COVID-19 vaccines require special end-to-end supply cold chain requirements, from manufacture, and transportation to warehouses and healthcare facilities. To sustain production, minimise wastage, and for vaccines to reach target populations, an efficient and resilient vaccine supply chain which is assisted by temperature monitoring technologies is imperative.
Global distribution of magnetic ripples and electron density fluctuations as observed by the Swarm satellites on the dayside and their relation to the rainfall estimated by the GSMaP
Authors: Iyemori Toshihiko , Aoyama Tadashi , Yokoyama Yoshihiro 
Published: March 2022

AbstractIn mid- and low-latitude ionospheric F-region on the dayside, magnetic field and electron density (Ne) fluctuations with amplitude smaller than a few nT and 1–2% of Ne, respectively, are commonly observed. Their spatial scale along satellite orbit is around 70–250 km. It is presumed that they are generated by the waves propagated from lower atmosphere. However, the mode of waves (acoustic wave or internal gravity wave) and their source are not yet clear. Among the possible sources, cumulus convection and/or associated rainfall are considered to be the strong candidates for the atmospheric wave generation. We use the rainfall estimated by the hourly Global Satellite Mapping of Precipitation (JAXA/GSMaP) as a proxy of lower atmospheric disturbance as the wave source, and compare the rainfall with the amplitude of magnetic fluctuations (magnetic ripples) and electron density fluctuations observed by the Swarm satellites. The data from April 2014 to July 2020 are used. The global distribution of rainfall estimated by the GSMaP and its seasonal variation have similarities with amplitude distribution of magnetic ripples and electron density fluctuations on the dayside. We calculate the ratio of their magnitude, i.e., amplitude of magnetic ripples or electron density fluctuations in rainfall cases to those in no-rainfall cases. Although the longitudinally averaged ratio is not very large but around 1.1–1.2 in ± 10– ± 50° Apex latitudes, it is clearly larger than 1.0. The ratio increases when the intensity of rainfall (mm/h) increases. These results indicate that a cumulous convection which causes rainfall is one of the main sources of atmospheric waves that produce magnetic ripples and electron density fluctuations commonly observed in the dayside ionosphere. Anticipating acoustic waves as the driver of magnetic ripples and electron density variations, a difference in the generation mechanism of electron density fluctuations from that of magnetic ripples is suggested even if their sources are common.
Graphical abstract