bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversity, community structure and potential functions of root-associated bacterial communities of different wheat (Triticum aestivum) cultivars under field conditions
Authors: Acuña Jacquelinne , Rilling Joaquin Ignacio , Inostroza Nitza , Manquian Javiera , Zhang Qian , Vadakattu Gupta , Jorquera Milko 
Published: November 2022

Abstract

Aim
Wheat (Triticum aestivum) microbiome is essential to its growth and adaptation under the current climatic crisis. Wheat breeding programs are mainly focused on developing pest and stress resistant cultivars; thus, plant genotype-by-microbiome interactions have gained attention. Thus, local wheat cultivars represent an opportunity to examine recruited and supporting bacterial communities under field conditions.
Method
In this study, we used Illumina MiSeq to explore the diversity, community structure and potential functions of root-associated bacterial communities of four wheat cultivars under field conditions.
Results
Proteobacteria was the most abundant phylum in root endosphere (51.1 to 74.4%) and rhizosphere samples (39.3 to 44.9%) across cultivars. Differences in alpha and beta diversity were observed in root endosphere and rhizosphere, independently of cultivar. Potassium was identified as the main factor driving rhizosphere microbiomes of wheat. Higher proportion of shared OTUs were found in rhizosphere (mainly Pseudomonas, Flavobacterium, and Janthinobacterium) compared with root-endosphere (dominated by Delftia, Acinetobacter, Stenotrophomonas, Kaistobacter) across cultivars. General predicted functional activities revealed chemoheterotrophy and aerobic chemoheterotrophy as more observed in the root endosphere environment, whereas nitrogen cycling was the more predicted in rhizosphere. Co-occurrence analysis revealed complex bacterial interactions in niche microbiomes identifying three (Comamonadaceae, Enterobacteraceae, Micrococcaceae) and four (Corynebacteraceae, Dermabacteraceae, Xanthomonadaceae, Staphylococcaceae) families as keystone taxa for root endosphere and rhizosphere, respectively.
Conclusions
Our findings suggest that under the same field conditions community differences are driven by niche, rather than cultivar. This might contribute to the development of new cultivars able to recruit specific bacterial communities.
Impact of Environmental Pollution on the Residents of Sharada From Tannery Industries, Kano, Northwestern Nigeria
Authors: Ekpa Dave Eleojo 
Published: November 2022

Abstract
Environmental pollution has become a worldwide challenge due to industrialisation and anthropogenic activities. Industrial effluents cause a wide scope of environmental problems with health hazards and these challenges are more complex and critical now than before, in developing countries like Nigeria. This study assessed the impact of environmental pollution and determined levels of heavy metals (HMs) in the effluents from tannery industries in Sharada phase I and II. The data used include laboratory test to determine heavy metals (Cd, Cr, Cu, Pb, Zn, Ni, Mn, Fe), Electrical Conductivity (EC) and Total Dissolved Solid (TDS) from industrial effluents. Interview was conducted and systematic field observation was employed to ascertain the perceptible impacts of the pollution on the environment. The finding reveals that concentrations of the physicochemical parameters are within the FEPA acceptable limits but the EC and TDS are above the limit. The pH value of the effluents is neutral. The major conspicuous and dangerous environmental challenges in the study area include air pollution and open drainages. The economic activities of the residents are negatively affected due to stink from the effluents and untidy environment. The environment has become detrimental to people’s health and the passerby. Thus, FEPA laws should be enforced, the industries should construct closed drainage, study on environmental impact assessment (EIA) should be conducted often to curtail the environmental challenges in the area in order to make the environment conducive for living, avoid further deterioration and ensure a sustainable hygienic environment.
Methodological Rigour in Preclinical Urological Studies: a 14-year systematic review to increase research quality and value
Authors: Park Seung Hyun , Lee Se Bee , Park Seoyeon , Kim Eunyoung , Pizzol Damiano , Trott Mike , Barnett Yvonne , Koyanagi Ai , Jacob Louis , Soysal Pinar , Veronese Nicola , Ippoliti Simona , Ghayda Ramy Abou , Thirumavalavan Nannan , Hijaz Adonis , Sheyn David , Pope Rachel , Conroy Britt , Nevo Amihay , Jaeger Irina , Shubham Gupta , Ilie Petre-Cristian , Lee Seung Won , Yon Dong Keon , Han Hyunho , Hong Sung Hwi , Shin Jae Il , Ponsky Lee , Smith Lee 
Published: November 2022

Abstract
Aims: Methodological rigour enhances reproducibility in preclinical studies and translation from preclinical to clinical studies. We aimed to investigate the prevalence and the trends of essential study design elements in preclinical urological studies, as well as key factors which may improve methodological rigour.
Methods and Results: PubMed database was searched, and all the resulting articles in preclinical urological articles published over the past 14-years were reviewed. Total 3768 articles met inclusion criteria. Data on study design elements and animal model used were collected. Citation density and journal impact factor was also examined as a surrogate marker of study influence. We performed analysis on prevalence of seven critical study design elements, and temporal patterns over 14 years. Randomization was reported in 50.0%, blinding in 15.0%, sample size estimation in 1.0%, inclusion of both sexes in 5.7%, statistical analysis in 97.1%, housing and husbandry in 47.7%, and inclusion/exclusion criteria in 5.0%. Temporal analysis showed that the implementation of these study design elements has increased, except for inclusion of both sexes and inclusion/exclusion criteria. Reporting study design elements were not associated with increased citation density.
Conclusions: The risk of bias is prevalent in 14-year publications describing preclinical urological research, and the quality of methodological rigour is poorly related to the journal impact factor or the citation of the article. Yet guidelines seem helpful in improving the research quality, because five study design elements (randomization, blinding, sample size estimation, statistical analysis, housing and husbandry) proposed by both NIH and ARRIVE guidelines have been either well reported or improved.
Systematic review registration: PROSPERO CRD42022233125
One-sentence summary: Research bias still exists in the fields in preclinical urology, but it is gradually improving.
Glycated Haemoglobin and Cardiovascular Complications in Non-Diabetic Haemodialysis Patients
Authors: Salem Ibrahim M. , Nawara Abdallah M. , Awadalla Mohamed M. , Ghonemy Tarek A. , Zanaty Mohamed A. , Borai Maher B. , Salem Mohamed I. , Sakla Niveen S. 
Published: November 2022

Abstract

Background
Cardiovascular disease is considered a common cause of morbidity and mortality in patients with end-stage renal disease on hemodialysis. Elevated levels of glycated hemoglobin (HbA1c) are associated with CVD in diabetic patients. However, there is lack of information regarding HbA1c and CVD risk in non-diabetic HD patients. This study aims to investigate the relationship between glycated hemoglobin levels and the risk for CVD in non-diabetic HD patients.
Methods
A prospective, cross-sectional study, which included 60 non-diabetic HD patients, 30 patients taking erythrocyte stimulating agents (ESA), and 30 patients with no-ESA. Each group was subdivided according to the presence or absence of CVD. All participants were subjected to full history taking, clinical examination, and laboratory investigations.
Results
Non-diabetic HD patients with CVD had higher mean ± SD HbA1c% (5.8 ± 0.2) and HOMA-IR (4.7 ± 0.7), than those with no-CVD (5.5 ± 0.3), and (4.2 ± 0.3) (p ˂0.05). Non-diabetic HD patients with CVD had longer dialysis duration, lower serum albumin, and HDL, but higher total cholesterol, CRP, Hb%, HbA1c%, and insulin resistance than those with no-CVD. HD patients who received higher doses of ESA therapy mean ± SD (80.8 ± 22.4) IU/Kg/Week had significantly lower HbA1c% (˂5%) than those who received lower doses (53.9 ± 23.8), who had HbA1c% (≥ 5.5–6%) (p˂0.05). There was a significantly positive correlation between the presence of CVD in HD patients and the HbA1c% levels (r = 0.492) (p˂0.05).
Conclusion
HbA1c can predict CVD in non-diabetic HD patients, and its lower levels are associated with lower CVD.
Statistical analysis for EUV dynamic spectra and their impact on the ionosphere during solar flares
Authors: Nishimoto Shohei , Watanabe Kyoko , Jin Hidekatsu , Kawai Toshiki , Imada Shinsuke , Kawate Tomoko , Otsuka Yuichi , Shinbori Atsuki , Tsugawa Takuya , Nishioka Michi 
Published: November 2022

Abstract

The X-rays and extreme ultraviolet (EUV) emitted during solar flares can rapidly change the physical composition of Earth's ionosphere, causing space weather phenomena. It is important to develop an accurate understanding of solar flare emission spectra to understand how it affects the ionosphere. We reproduced the entire solar flare emission spectrum using an empirical model and physics-based model, and input it into the earth’s atmospheric model, GAIA to calculate the total electron content (TEC) enhancement due to solar flare emission. We compared the statistics of nine solar flare events and calculated the TEC enhancements with the corresponding observed data. The model used in this study was able to estimate the TEC enhancement due to solar flare emission with a correlation coefficient greater than 0.9. The results of this study indicate that the TEC enhancement due to solar flare emission is determined by soft X-ray and EUV emission with wavelengths below 35 nm. The TEC enhancement is found to be largely due to the change in the soft X-ray emission and EUV line emissions with wavelengths such as Fe XVII 10.08 nm, Fe XIX 10.85 nm and He II 30.38 nm.
Microfacies analysis and paleolimnology of latest Pleistocene - Holocene lacustrine sediments of Faiyum paleolake, Egypt
Authors: Hamdan M.A , Afify A. B. , Osman R. , Hamed R.A. , Hassan F.A. , Flower R.J. , Mabrouk M.S. 
Published: November 2022

Abstract
The geomorphological and geological settings of Faiyum Depression make its lake sediments relevant to both the paleoclimate and paleohydrology of North Africa in general and Egypt in particular. Faiyum Depression is an endorheic basin that received fresh water from the Nile River during seasonal high flows throughout most of the late Pleistocene and Holocene. A detailed analysis of the textural and microfacies of lacustrine sediments were carried out. The sediment at the base of core F3-08, represent the last shirking phase of the previously huge late Pleistocene Lake phase with high beaches at c. 45m ASL. This phase represents ended by complete dissociation of the lake and prevailing of desert condition with accumulation of aeolian sand. An early Holocene freshwater mega-lake initially filled almost the whole Faiyum Depression and their beaches are existed at elevation of c. 25m ASL and since that time lake levels have fluctuated markedly leading to the small saline lake (Lake Qarun). During the Early and Middle Holocene, lake level changes were largely influenced by changing Nile floods driven by African monsoons and Westerlies affecting the African Nile headwaters. Detailed examination of sediment structures using high resolution microfacies differentiation in a continuous Holocene sediment core revealed the fine detail of sedimentological complexities. These are primarily attributed to changes in sediment supply, sedimentological conditions and sediment accumulation rates as the lake system responded to environmental modifications caused by both climate and by ancient people.
hMSH2 Coordinated with the Expression of E2F1 Promotes Platinum Response in Epithelial Ovarian Cancer
Authors: Hua Tian , Zhang Bao-ying 
Published: November 2022

Abstract
Purpose Previous studies have demonstrated that hMSH2 deficiency decreased platinum response in EOC. This study is to explore the underlying mechanism that regulates hMSH2 expression and drug susceptibility.
Methods Transcription factors (TFs) that potentially regulate hMSH2 were predicted by bioinformatics analysis. RT-qPCR, Western blot, and luciferase assay were carried out in ovarian cancer cell lines to verify the identified TF. Expressions of the identified TF were modulated via overexpression or knockdown, and the corresponding cellular responses to the cisplatin were examined. Kaplan-Meier analysis was used to verify the correlation between the identified TF and EOC patients’ clinical outcomes.
Results The common transcription factor E2F1 was identified to bind the promoter region of the hMSH2 gene and presented a positive correlation with the expression of hMSH2 in the public database. RT-PCR and Western blot verified the coordinate expression of E2F1 and hMSH2 in ovarian cancer cells. The luciferase assay also validated the regulation of hMSH2 by E2F1. The expression level of E2F1 is also found to correlate with cisplatin susceptibility in vitro. The Kaplan-Meier analyses of 77 EOC patients showed that low E2F1 expression was associated with worse survival.
Conclusion E2F1 regulated MSH2 expression could play a vital role in the drug resistance of platinum-based treatments for ovarian cancer patients. The complex role of E2F1 in various cancers still needs to be further explored.
A quantitative interpretation of oxidative protein folding activity in Escherichia coli
Authors: Rettenbacher Lukas A. , Haar Tobias von der 
Published: November 2022

Abstract
Background
Escherichia coli is of central interest to biotechnological research and a widely used organism for producing proteins at both lab and industrial scales. However, many proteins remain difficult to produce efficiently in E. coli. This is particularly true for proteins that require post translational modifications such as disulfide bonds.
Results
In this study we develop a novel approach for quantitatively investigating the ability of E. coli to produce disulfide bonds in its own proteome. We summarise the existing knowledge of the E. coli disulfide proteome and use this information to investigate the demand on this organism’s quantitative oxidative folding apparatus under different growth conditions. Furthermore, we built an ordinary differential equation-based model describing the cells oxidative folding capabilities. We use the model to infer the kinetic parameters required by the cell to achieve the observed oxidative folding requirements. We found that the cellular requirement for disulfide bonded proteins changes significantly between growth conditions. Fast growing cells require most of their oxidative folding capabilities to keep up their proteome while cells growing in chemostats appear limited by their disulfide bond isomerisation capacities.
Conclusion
This study establishes a novel approach for investigating the oxidative folding capacities of an organism. We show the capabilities and limitations of E. coli for producing disulfide bonds under different growth conditions and predict under what conditions excess capability is available for recombinant protein production.
Immunomodulatory, anti-infammatory and antioxidant activities of aqueous and ethanolic extracts of Cissus quadrangularis Linn. (Vitaceae) in chronic pain
Authors: Feigni Olga Youyi Marcelle , Mbiantcha Marius , Yousseu William Nana , Tsafack Gonzal Eric , Djuichou Flore Nguepnang Stephanie , Noungoua Chrétien Mbeugangkeng , Atsafack Gisèle Mboudem Lylie , Ateufack Gilbert 
Published: November 2022

Abstract

Cissus quadrangularis Linn. (Vitaceae) is a plant used to treat injured tendons, broken bones, asthma, stomach ache, scurvy and digestive disorders. The present study purposed to evaluate the antihyperalgesic effects (in vivo) and the immunomodulatory, antioxidant and anti-inflammatory properties (in vitro) of aqueous and ethanolic extracts of Cissus quadrangularis (C. quadrangularis). Immunomodulatory (chemiluminescence, cytokines and cell proliferation), anti-inflammatory (protein denaturation, 5-lipoxygenase, cyclooxygenase 1 and 2) and antioxidant (DPPH, ABTS and NO) tests were performed in vitro, while the anti- hyperalgesic (vincristine) investigations were conducted in vivo on Wistar rats. The results revealed that extracts developed immunomodulatory activity by inhibiting the production of ROS (intracellular/extracellular), of TNFα, IL-1β, IL-6 as well as inhibiting cell proliferation, and by stimulating the production of IL-10. The anti-inflammatory activity of the extracts was demonstrated by an inhibition of 5-LOX, protein denaturation and cyclooxygenases 1 and 2. In addition, extracts showed interesting scavenging effects, attesting their antioxidant potential. The extracts administered to the animals (180 and 360 mg/kg) inhibited (p < 0.001) hyperalgesia and allodynia in animals. These extracts also led to the reduction in serum and sciatic nerve levels of TNFα, IL-1β and IL-6, as well as to an increase in cell growth factors (NGF and IGF) production of treated animals. These results suggest that extracts of C. quadrangularis use immunomodulatory, anti-infammatory and antioxidant capacity to prevent and/cure painful neuropathy after vincristine administration. C. quadrangularis is therefore a promising natural substance for the management of neuropathic pain.
Atomic-level Chemical Reaction Promoting External Quantum Efficiency of Organic Photodetector Exceeding 10^8% for Weak-Light Detection
Authors: Shi Linlin , Zhu Yizhi , Guohui Li , Ji Ting , Wang Wenyan , Zhang Ye , Wu Yukun , Hao Yuying , Wang Kaiying , Zhang Fujun , Yuan Jun , zou yingping , Ong Beng , Zhu Furong , Cui Yanxia 
Published: November 2022

Abstract
Low-cost, solution-processed photomultiplication organic photodetectors (PM-OPDs) with external quantum efficiency (EQE) above unity have attracted enormous attention. However, their weak-light detection is unpleasant because the anode Ohmic contact causes exacerbation in dark current. Here, we introduce atomic-level chemical reaction in PM-OPDs which can simultaneously suppress dark current and increase EQE via depositing a 0.8 nm thick Al2O3 by atomic layer deposition. Suppression in dark current mainly originates from the built-in anode Schottky junction as a result of work function decrease of hole-transporting layer of which the chemical groups can react chemically with the bottom surface of Al2O3 layer at the atomic-level. Such strategy of suppressing dark current is not adverse to charge injection under illumination; instead, responsivity enhancement is realized because charge injection can shift from cathode to anode, of which the neighborhood possesses increased photogenerated carriers. Consequently, weak-light detection limit of the Al2O3 treated PM-OPD reaches a remarkable level of 2.5 nW/cm2, while that of the control is 25 times inferior. Meanwhile, the PM-OPD yields a record high EQE and responsivity of 4.31×108% and 1.85×106 A/W, respectively, outperforming all other polymer-based PM-OPDs.
Real-time Implementation and Experimental Verification of Dual-Switch Coupled Inductor (DSCI) Converter based BLDC Drive for Electric Vehicle Applications
Authors: Chandran Praveenkumar , Mylsamy Kaliamoorthy , Umapathy Prabha S 
Published: November 2022

Abstract

Large heat output and burning have recently emerged as critical challenges in electric vehicles (EV). The main causes of this issue are the use of spark-propelled motors and in-built complicated circuits. This research attempts to address the aforementioned issues by providing a reduced switch converter for Brush-Less DC (BLDC) motors. A Dual-Switch Coupled Inductor (DSCI) converter is proposed based on the BLDC drive for electric vehicle (EV) applications. Because the DSCI converter has only two switches, the need for a gate-driven circuit is greatly minimized, which is responsible for high heat dissipation and complicates the circuit. Theoretical analysis is performed on the operating principles, switching frequencies, component design concerns, maximum voltage gain, and efficiency, as well as simulations using Matlab® Simulink. The efficiency of the DSCI converter obtained is 98.23%. The converter is used to drive a 0.75-kW BLDC motor, and the simulation results are validated with a hardware prototype model at 3000 rpm. At the end of the paper, the explored results for both hardware and simulation and comparative studies with earlier work are described in detail.
Copper(II) coordination polymer based on L-arginine as a supramolecular hybrid inorganic-organic material - synthesis, structural, spectroscopic and magnetic properties
Authors: Musioł Karol , Janczak Jan , Helios Katarzyna , Witwicki Maciej , Fitta Magdalena , Pełka Robert , Wojciechowska Agnieszka 
Published: November 2022

Abstract
We report the synthesis and structural, spectroscopic and magnetic properties of new 1D coordination polymeric complex of the formulae {[Cu(µ−l−Arg)2]SO4⋅1.5H2O}n (1) that contains asymmetric µ − O,O’ carboxylic bridge linking distorted square-pyramidal [Cu(µ−l−Arg)2]2+ coordination units. In 1D the syn − anti − µ2 − η1:η1 zig-zag polymer conformation, the adjacent Cu(II) ions are distanced by 5.707 Å, and the subsequent Cu∙∙∙Cu proximity in 1D− coordination chain equals 6.978 Å. Detailed interpretation of IR and Raman spectra of l−arginine and 1 has been performed. The values of EPR parameters (gx=2.059, gy=2.075, gz=2.228) indicate nearly of axial local symmetry of Cu(II) coordination sphere (elongated square pyramidal geometry) and correspond to dx2−y2 orbital of unpaired electron ground state. The single broad band at 16200 cm–1, characteristic for d − d transition, is assigned to the dominant dublet-dublet 2B1g(dx2−y2)→2Eg(dyz≈dxz) transition. Magnetic susceptibility measurement has revealed ferromagnetic coupling between the Cu(II) ions within the 1D−coordination chain, while the intermolecular coupling is antiferromagnetic.
Genetic structure and variation of Liobagrus nigricauda of the upper Yangtze River basin based on cytochrome b
Authors: Jia Xiang-Yang , Li Wei , Tu Bin 
Published: November 2022

Abstract
Liobagrus nigricauda is endemic to the upper Yangtze River basin. By using the fast isolation by amplified fragment polymorphism of sequences containing cytochrome b, we identified the genetic variation in 6 populations of L. nigricauda. A total 21 nucleotide sites were detected in 1086 bp from cytochrome b, and 16 haplotypes were defined from 145 sequences. Nucleotide diversity index was 0.00087, and haplotype diversity index was 0.26858. Hierarchical analysis of molecular variance (AMOVA) showed that the variation from within populations was 93.03%. And the mainstream populations of the upper Yangtze River had preliminary genetic differentiation with tributary.