bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a Dedicated Software “Elements™ Spine SRS, Brainlab®” for Target Volume Definition in the Treatment of Spinal Bone Metastases With Stereotactic Body Radiotherapy
Authors: Rogé Maximilien , Henni Ahmed Hadj , Neggaz Yasmine Adda , Mallet Romain , Hanzen Chantal , Dubray Bernard , Colard Elyse , Gensanne David , Thureau Sébastien 
Published: May 2022

IntroductionStereotactic body radiotherapy (SBRT) is a treatment option for spine metastases. The International Spine Radiosurgery Consortium (ISRC) has published consensus guidelines for target delineation in spine SBRT. A new software called Elements™ Spine SRS by Brainlab® that includes the module Elements SmartBrush Spine (v3.0, Munich, Germany) has been developed specifically for SBRT treatment of spine metastases, and the latter provides the ability to perform semiautomatic clinical target volume (CTV) generation based on gross tumor volume (GTV) localization and guidelines. The aims of our study were to evaluate this software by studying differences in volumes between semiautomatic CTV contours compared to manual contouring performed by an expert radiation oncologist and to determine the dosimetric impact of these differences on treatment plans.MethodsA total of 35 volumes (“Expert GTV” and “Expert CTV”) from 30 patients were defined by a single expert. A semiautomatic definition of these 35 CTVs based on the location of “Expert GTV” and following ISRC guidelines was also performed in Elements SmartBrush Spine (“Brainlab CTV”). The spatial overlap between “Brainlab” and “Expert” CTVs was calculated using the Dice similarity coefficient (DSC). We considered a threshold of 0.80 or above to indicate that Elements SmartBrush Spine performed very well with adequate contours for clinical use. Two dosimetric treatment plans, each corresponding to a specific planning target volume (PTV; Expert PTV, Brainlab PTV), were created for 11 patients.ResultsWe showed that “Brainlab CTV” and “Expert CTV” mean volumes were 29.8 ± 16.1 and 28.7 ± 15.7 cm3, respectively (p = 0.23). We also showed that the mean DSC for semiautomatic contouring relative to expert manual contouring was 0.85 ± 0.08 and less than 0.80 in five cases. For metastases involving the vertebral body only (n = 13,37%), the mean DSC was 0.90 ± 0.03, and for ones involving other or several vertebral regions (n = 22.63%), the mean DSC was 0.81 ± 0.08 (p &lt; 0.001). The comparison of dosimetric treatment plans was performed for equivalent PTV coverage. There were no differences between doses received by organs at risk (spinal cord and esophagus) for Expert and Brainlab PTVs, respectively.ConclusionThe results showed that the semiautomatic method had quite good accuracy and can be used in clinical routine even for complex lesions.
A Novel and Robust Prognostic Model for Hepatocellular Carcinoma Based on Enhancer RNAs-Regulated Genes
Authors: Zhang Wei , Chen Kegong , Tian Wei , Zhang Qi , Sun Lin , Wang Yupeng , Liu Meina , Zhang Qiuju 
Published: May 2022

Evidence has demonstrated that enhancer RNAs (eRNAs) play a vital role in the progression and prognosis of cancers, but few studies have focused on the prognostic ability of eRNA-regulated genes (eRGs) for hepatocellular carcinoma (HCC). Using gene expression profiles of HCC patients from the TCGA-LIHC and eRNA expression profiles from the enhancer RNA in cancers (eRic) data portal, we developed a novel and robust prognostic signature composed of 10 eRGs based on Lasso-penalized Cox regression analysis. According to the signature, HCC patients were stratified into high- and low-risk groups, which have been shown to have significant differences in tumor immune microenvironment, immune checkpoints, HLA-related genes, DNA damage repair-related genes, Gene-set variation analysis (GSVA), and the lower half-maximal inhibitory concentration (IC50) of Sorafenib. The prognostic nomogram combining the signature, age, and TNM stage had good predictive ability in the training set (TCGA-LIHC) with the concordance index (C-index) of 0.73 and the AUCs for 1-, 3-, and 5-year OS of 0.82, 0.77, 0.74, respectively. In external validation set (GSE14520), the nomogram also performed well with the C-index of 0.71 and the AUCs for 1-, 3-, and 5-year OS of 0.74, 0.77, 0.74, respectively. In addition, an important eRG (AKR1C3) was validated using two HCC cell lines (Huh7 and MHCC-LM3) in vitro, and the results demonstrated the overexpression of AKR1C3 is related to cell proliferation, migration, and invasion in HCC. Altogether, our eRGs signature and nomogram can predict prognosis accurately and conveniently, facilitate individualized treatment, and improve prognosis for HCC patients.
Identification of LSM Family Members as Novel Unfavorable Biomarkers in Hepatocellular Carcinoma
Authors: Zhuang Hongkai , Chen Bo , Tang Chenwei , Chen Xinming , Tan Wenliang , Yang Lei , Xie Zhiqin , Ma Xiaowu , Wang Qingbin , Zhang Chuanzhao , Shang Changzhen , Chen Yajin 
Published: May 2022

BackgroundSmith-like (LSM) family members play critical roles in multiple oncologic processes in several types of malignancies. The study on LSM family members of HCC might provide new insights into the tumorigenesis and therapeutic strategies of HCC.MethodsThe clinical significance and oncologic biological functions of LSM family members were assessed through multiple bioinformatics methods and in vitro studies. The potential correlation between LSM family members and tumor immunity was also investigated using single sample gene set enrichment analysis (ssGSEA) and the ESTIMATE algorithm.ResultsLSM family member overexpression in HCC was significantly correlated with poor clinical outcomes such as higher TNM stage, advanced histologic grade, and worse prognosis. A risk score system based on LSM5, LSM10, LSM12, and LSM14B showed a reliable predictive ability for OS of HCC patients. Functional enrichment analysis demonstrated that LSM family members overexpressed were all involved in cell cycle related biological processes. Besides, LSM12, LSM14A, and LSM14B were found to be significantly associated with PI3K-Akt-mTOR and T cell receptor signaling pathways. Tumors with LSM12, LSM14A, and LSM14B overexpression exhibited lower infiltration of activated CD8+ T cells with declined cytolytic activity and immune score, but increased infiltration of Th2 cells and Th2/Th1. LSM12, LSM14A, and LSM14B overexpression is also associated with higher tumor-related immune checkpoints (e.g., PD-L1, B7-H3, and PVR) expression and increased therapeutic insensitivity to immune checkpoint blockade (ICB). Moreover, the knockdown of LSM12, LSM14A, and LSM14B significantly inhibited the proliferation and invasion of HCC cells.ConclusionThis study systematically investigated the expression pattern and biological values of LSM family members in HCC and identified LSM family members as novel therapeutic targets in HCC.
Development and Validation of a Prediction Model for Positive Margins in Breast-Conserving Surgery
Authors: Zhao Rong , Xing Jun , Gao Jinnan 
Published: May 2022

BackgroundThe chances of second surgery due to positive margins in patients receiving breast-conversing surgery (BCS) were about 20-40%. This study aims to develop and validate a nomogram to predict the status of breast-conserving margins.MethodsThe database identified patients with core needle biopsy-proven ductal carcinoma in situ (DCIS) or invasive breast carcinoma who underwent BCS in Shanxi Bethune Hospital between January 1, 2015 and December 31, 2021 (n = 573). The patients were divided into two models: (1) The first model consists of 398 patients who underwent BCS between 2015 and 2019; (2) The validation model consists of 175 patients who underwent BCS between 2020 and 2021. The development of the nomogram was based on the findings of multivariate logistic regression analysis. Discrimination was assessed by computing the C-index. The Hosmer-Lemeshow goodness-of-fit test was used to validate the calibration performance.ResultsThe final multivariate regression model was developed as a nomogram, including blood flow signals (OR = 2.88, p = 0.001), grade (OR = 2.46, p = 0.002), microcalcifications (OR = 2.39, p = 0.003), tumor size in ultrasound (OR = 2.12, p = 0.011) and cerbB-2 status (OR = 1.99, p = 0.042). C-indices were calculated of 0.71 (95% CI: 0.64-0.78) and 0.68 (95% CI: 0.59-0.78) for the modeling and the validation group, respectively. The calibration of the model was considered adequate in the validation group (p &gt; 0.05).ConclusionWe developed a nomogram that enables the estimation of the preoperative risk of positive BCS margins. Our nomogram provides a valuable tool for identifying high-risk patients who might have to undergo a wider excision.
Vision-Related Quality of Life in Patients With Atypical Optic Neuritis
Authors: Jiang Zhaocai , Qian Haiyan , Wei Shihui 
Published: May 2022

AimTo evaluate the vision-related quality of life (QOL) in patients with atypical optic neuritis through the 39-item National Eye Institute Visual Function Questionnaire (NEI VFQ-39).MethodsFifty-seven patients with atypical optic neuritis were scheduled in the research unit from June 1 to December 31, 2019. Besides collecting the clinical data, NEI VFQ-39, the Chinese version, was applied to all subjects. The NEI VFQ-39 subscale item scores were compared among subgroups divided by different classifications, and a correlation analysis of the NEI VFQ-39 scores and best-corrected visual acuity (BCVA) for better-seeing and worse-seeing eyes was performed.ResultsThe mean age of scheduled patients was 34.3 ± 12.4 years, with the majority being female (70.2%). The mean composite score was 58.62 ± 17.62. Twenty-nine (50.9%) patients were affected binocularly, and the most subscale scores and composite scores of binocular incidences were lower than those of monocular incidence significantly. However, there was no statistically significant difference in composite scores between patients with and without periocular pain. The patients with worse BCVA in the better-seeing eye have lower scores than those with better BCVA in the better-seeing eye. Most NEI VFQ-39 scores have strong correlations with the BCVA for better-seeing and worse-seeing eyes.ConclusionAtypical optic neuritis has a significant and comprehensive influence on patients' visual function outcomes and quality of life associated with vision. Improving their BCVA in the better-seeing eye can improve their vision-related QOL.
Stillbirth and Associated Factors Among Women Who Gave Birth at Hiwot Fana Specialized University Hospital, Harar, Eastern Ethiopia
Authors: Mengistu Seble , Debella Adera , Mulatu Teshale , Mesfin Firehiwot , Danusa Kababa Temesgen , Dheresa Merga 
Published: May 2022

BackgroundStillbirth, which accounts for half of all the perinatal mortality, is not counted on policy, program, and investment agendas around the globe. It has been underestimated public health burden, particularly in developing countries. Ethiopia is among the top countries with a large prevalence of stillbirth in the world. However, there is a dearth of study on the current magnitude of stillbirth in the study area. Therefore, this study intended to assess the prevalence of stillbirth and its associated factors to bridge the gap.MethodsA hospital-based retrospective study was conducted from 1 to 28 February 2019 and data were collected by reviewing the chart records of all the women who gave birth in the past 2 years (January 2016 to December 2018) at Hiwot Fana Specialized University Hospital. Data were entered into EpiData version 4.2.0.0 software and transported to SPSS version 23 for analysis. Descriptive statistics such as frequency, mean, and SDs were generated. Determinants of stillbirth were analyzed using a binary logistic regression and presented by adjusted odds ratio (AOR) with a 95% CI.ResultsThe prevalence of stillbirth was 14.5% (95% CI: 11.7%, 17.6%). Low birth weight (AOR = 2.42, 95% CI: 1.23–4.76), prematurity (AOR = 2.10, 95% CI: 1.10–4.01), premature rupture of membranes (AOR = 2.08, 95% CI: 1.14–3.77), antepartum hemorrhage (AOR = 3.33, 95% CI: 1.66–6.67), obstructed labor (AOR = 2.87, 95% CI: 1.48–5.56), and preeclampsia (AOR = 2.91, 95% CI: 1.28–6.62) were an independently associated with stillbirth.ConclusionThe prevalence of stillbirth in this study was high. Low birth weight, preterm birth, premature rupture of membranes, antepartum hemorrhage, obstructed labor, and preeclampsia were independently associated with a stillbirth. Therefore, much study is needed involving different stakeholders to reduce stillbirths by improving the health status of women through the provision of quality maternal care including referral systems.
Antimicrobial Dosing Recommendations in Pediatric Continuous Renal Replacement Therapy: A Critical Appraisal of Current Evidence
Authors: Stitt Gideon , Dubinsky Samuel , Edginton Andrea , Huang Yuan-Shung V. , Zuppa Athena F. , Watt Kevin , Downes Kevin 
Published: May 2022

ObjectivesContinuous renal replacement therapy (CRRT) is commonly employed in critically ill children and is known to affect antimicrobial pharmacokinetics. There is a lack of readily available, evidence-based antimicrobial dosing recommendations in pediatric CRRT. This study aims to quantify commonly used antimicrobial drugs in pediatric CRRT and identify gaps between contemporary literature-based dosing recommendations and those presented in a frequently used dosing reference.MethodsThe Pediatric Health Information System (PHIS) database was queried from July 1, 2018 through June 30, 2021 to identify admissions in which antimicrobials were billed on the same day as CRRT. Drugs of interest were selected if at least 10% of admission involved administration on at least one CRRT day, with additional clinically important antimicrobials selected by the authors. A comprehensive literature search was performed to identify antimicrobial pharmacokinetic (PK) studies in children for each selected drug. For identified articles, dosing recommendations were extracted and compared to those in a popular tertiary dosing reference (Lexi-Comp Online database). The level of agreement of the dosing recommendations was assessed.Results77 unique antimicrobial agents were identified amongst 812 admissions from 20 different PHIS hospitals. Fifteen antimicrobials were billed on the same day as CRRT in ≥10% of admissions, with 4 additional drugs deemed clinically relevant by the authors. Twenty PK studies were identified for these 19 drugs, and dosing recommendations were included in 8 (42.1%) of them. Seventeen agents (89.5%) had some type of CRRT-specific dosing guidance in Lexi-Comp, with only 1 directly based on a pediatric CRRT study. For the 8 agents with PK data available, Lexi-Comp recommendations matched primary literature dosing guidance in 3 (37.5%). Two (25%) lacked agreement between the Lexi-Comp and primary literature, and the remaining 3 (37.5%) had partial agreement with multiple dosing regimens suggested in the primary literature and at least one of these regimens recommended by Lexi-Comp.ConclusionSignificant gaps exist in the data supporting antimicrobial dosing recommendations for children receiving CRRT. Future studies should focus on antimicrobial dosing in pediatric CRRT, emphasizing provision of robust data from which dosing recommendations can be promptly incorporated into tertiary dosing references.
A Novel Egg-In-Cube System Enables Long-Term Culture and Dynamic Imaging of Early Embryonic Development
Authors: Dave Mohit , Levin Joshua , Ruffins Seth Walter , Sato Yuki , Fraser Scott , Lansford Rusty , Kawahara Tomohiro 
Published: May 2022

The avian egg is a closed system that protects the growing embryo from external factors but prevents direct observation of embryo development. Various culture systems exist in the literature to study the development of the embryo for short periods of incubation (from 12 h up to a maximum of 60 h of egg incubation). A common flaw to these culture techniques is the inability to culture the unincubated avian blastoderm with intact tissue tensions on its native yolk. The goal of this work is to create a unique novel egg-in-cube system that can be used for long-term quail embryo culture initiated from its unincubated blastoderm stage. The egg-in-cube acts as an artificial transparent eggshell system that holds the growing embryo, making it amenable to microscopy. With the egg-in-cube system, quail embryos can be grown up to 9 days from the unincubated blastoderm (incubated in air, 20.9% O2), which improves to 15 days on switching to a hyperoxic environment of 60% O2. Using transgenic fluorescent quail embryos in the egg-in-cube system, cell movements in the unincubated blastoderm are imaged dynamically using inverted confocal microscopy, which has been challenging to achieve with other culture systems. Apart from these observations, several other imaging applications of the system are described in this work using transgenic fluorescent quail embryos with upright confocal or epifluorescence microscopy. To demonstrate the usefulness of the egg-in-cube system in perturbation experiments, the quail neural tube is electroporated with fluorescent mRNA “in cubo”, followed by the incubation of the electroporated embryo and microscopy of the electroporated region with the embryo in the cube. The egg-in-cube culture system in combination with the “in cubo” electroporation and dynamic imaging capabilities described here will enable researchers to investigate several fundamental questions in early embryogenesis with the avian (quail) embryo on its native yolk.
Borderline Pathological Celebrity Worship and Impulsive Buying Intent: Mediating and Moderating Roles of Empathy and Gender
Authors: Chen Outong , Zhao Xiaojing , Ding Dongxing , Zhang Yifan , Zhou Hongbo , Liu Ranran 
Published: May 2022

The present study constructed a moderated mediation model to examine whether borderline pathological celebrity worship may be associated with higher levels of impulsive buying intent. The mediating role of empathy and the moderating role of gender were also examined. A total of 1,319 participants recruited from a college through the campus network. The results indicated that (1) borderline pathological celebrity worship could positively predict individuals’ impulsive buying intent; (2) the predictive effect of borderline pathological celebrity worship on impulsive buying intent could be mediated by empathy; and (3) gender moderated the predictive effect of borderline pathological celebrity worship on impulsive buying intent. Specifically, relatively higher levels of borderline pathological celebrity worship were found to be associated with higher impulsive buying intent among men. However, this was relatively less significant among women.ConclusionThe results contribute to previous findings by demonstrating that borderline pathological celebrity worship could affect impulsive buying intent. Moreover, the mediating role of empathy and the moderating role of gender were also revealed.
Adaptation of Work Values Instrument in Indonesian Final Year University Students
Authors: Sulistiobudi Rezki Ashriyana , Hutabarat Harlin Nikodemus 
Published: May 2022

BackgroundOne of the preferences working in the Generation Z is based on their motivational work values. The relevance of job choices with the work values will contribute to student career planning. The work value instrument among generations is one of the popular instruments used to measure final year students' work value, yet few studies of the psychometric properties of non-English language versions of this instrument. This study's objectives were to adapt a questionnaire of work value in Indonesian final year university students.MethodsThe number of participants in this study was 316 students in Indonesia, comprised of final year students from various majors who were selected by quota sampling. The instrument consisted of 5 dimensions of value, including leisure, extrinsic rewards, intrinsic rewards, altruistic rewards, and social rewards. The reliability analysis was performed using McDonald's Omega, the evidence of validity was obtained from test content, internal structure through confirmatory factor analysis (CFA), and evidence-based in relation to other variable has conducted the correlation between work value and career development learning using the Pearson's correlation coefficient.ResultsThe results showed that the work values instrument had good psychometric properties, including good reliability, good content validity, and internal structure. In CFA, the two-factor structure showed satisfactory model fit. Moreover, the correlation of work value with career development learning builds stronger validity evidence on this instrument.ConclusionThe adapted instrument can be used practically to identify work value preferences of final year students to help them choose a work preference and setup the career planning before graduating. The result could be of interest for the researcher in work value, motivational work, and career areas in higher education. To the best of our knowledge, there have been no reports about the adaptation of work value instruments in Indonesian final year university students.
COVID-19 Preventive Behaviors and Influencing Factors in the Thai Population: A Web-Based Survey
Authors: Rojpaisarnkit Kunwadee , Kaewpan Wonpen , Pengpid Supa , Peltzer Karl 
Published: May 2022

ObjectivesTo identify factors influencing COVID-19 preventive behaviors among the Thai population.MethodsA cross-sectional web-based survey was used. A total of 6,521 Thai people completed the survey. The multiple linear regression analysis was performed to identify factors that influenced coronavirus disease 2019 (COVID-19) preventive behaviors. The Predisposing, Reinforcing, and Enabling Constructs in Educational Diagnosis and Evaluation (PRECEDE) model was applied to propose factors influencing COVID-19 preventive behaviors.ResultsThe factors that mostly influenced COVID-19 prevention behaviors when controlling for the other variables are social support (β = 0.173, p &lt; 0.001) follow by age (β = 0.162, p &lt; 0.001), flu-like symptoms (β = 0.130, p &lt; 0.001), gender (β = −0.084, p &lt; 0.001), perceived risk of exposure (β = 0.035, p &lt; 0.05), lock down policy (β = 0.029, p &lt; 0.05), and residential area (β = −0.027, p &lt; 0.05), respectively. These factors explained 52% of the COVID-19 preventive behaviors in Thai population.ConclusionThe result of this study was a foundation for further studies on different groups of people to develop different strategies to adopt preventive behaviors to reduce the spread of the COVID-19.