bluuebunny/crossref_metadata_2025_split huggingface dataset:
COPING STRATEGIES AMONG HISPANIC AND LATINX DEMENTIA CAREGIVERS
Authors: Colmenares Nury Rodriguez , Wang Danny , Alvarez Loreli , Estes Katie , Bibriescas Natashia , Puga Frank 
Published: January 2025

Abstract
The objective of this study was to examine coping strategies employed by Hispanic and Latinx (H&L) family caregivers (FCGs) of people living with dementia (PLWD) and associations with sociodemographic characteristics. A nonparametric analysis was used to analyze data from an ongoing study on the daily experiences of H&L dementia caregivers, entitled Nuestros Días. A total of 52 H&L FCGs were assessed using Brief-COPE. Most H&L FCGs identified as female (92.2%), with a mean age of 56 years (SD = 8.6), were adult children caring for biological parent (59.6%), and provided care for 3 or more years (61.5%). Most PLWD were female (68%) with a mean age of 78 years (SD= 8.6). Overall coping strategies most frequently utilized by H&L FCGs included acceptance (M= 3.14, SD= 1), religion (M= 2.98, SD= 1), positive reframing (M=2.91, SD=0.9), and planning (M=2.8, SD=1). Conversely, substance use (M=1.13, SD=0.3), behavioral disengagement (M=1.39, SD=0.72), denial (M=1.44, SD=0.8), and humor (M=1.47, SD=0.8) were the least utilized by H&L FCGs. The use of acceptance as a coping strategy was found to have a significant association with employment status with working caregivers more frequently endorsing acceptance as a coping strategy (p=.04; Cramer’s V=.409). These findings suggest that H&L caregivers utilize specific coping strategies and that sociodemographic factors, such as employment, may influence coping strategies use d. Additional studies are needed to examine the relationship between additional contextual factors (e.g., caregiving-related stressors and burden) and types of coping strategies used by H&L FCGs to inform ecologically valid and cultural interventions.
ACTIVE IMMUNIZATION AGAINST MYOSTATIN AND ACTIVIN A IMPROVES SKELETAL MUSCLE STRENGTH DESPITE GH/IGF-1 DEFICIENCY
Authors: Mansoor Mishfak Abdullah Mohamed , Ashiqueali Sarah , Alam Md Tanjim , Lasseter Amanda , Thibodeaux Brett , Masternak Michal 
Published: January 2025

Abstract
Muscle wasting is a significant hurdle in patients due to immobilization, prolonged bed rest, and injury. In particular, the older population develops sarcopenia, characterized by progressive loss of muscle strength due to natural aging. However, there are no FDA-approved drugs for sarcopenia, and it is critical to explore novel therapeutics. Myostatin inhibition is one such strategy that has had varying degrees of success previously, yet the desired levels of muscle strength have not been achieved. Here, we employed a novel vaccine (V13) that generates anti-myostatin antibodies and another (V07) that generates anti-activin A; myostatin and activin A regulate muscle growth negatively. We administered seven doses of V13, V07, and V35 (generates both anti-myostatin and anti-activin A) to long-living, GH-deficient Ames dwarf mice (df/df) and their normal littermate controls (N/df). By week 24 post-first dose, we saw increased forelimb grip strength in V13, V07, and V35 vaccinated df/df mice and V07 and V35 vaccinated N/df mice. Additionally, almost 40% of the vaccinated df/df mice completed the accelerating rotarod trials, compared to the 20% of placebo-treated counterparts. We observed increased lean body mass and reduced fat mass in vaccinated N/df mice, yet surprisingly there were no changes in lean and fat mass in df/df mice. In summary, our results indicate that while myostatin and activin A inhibition play an intimate role in fat metabolism in the presence of GH/IGF-1, this immunization strategy improves muscle motor performance in GH/IGF-1 deficient mice providing promising treatments in older animals regardless age-associated suppression of GH/IGF-1 axis.
ACUTE NAD+ SUPPLEMENTATION MODULATES SYSTEMIC INFLAMMATION IN A LPS-INDUCED DELIRIUM MOUSE MODEL
Authors: Chui M K Kirsten , Kumaar Prasanna Vadhana Ashok , Verdin Eric , Newman John 
Published: January 2025

Abstract
Delirium is a serious neuropsychiatric condition that lacks an effective treatment intervention. A confusional state often brought on by acute illness, delirium is associated with acute inflammation and metabolic dysfunction. NAD+ is a metabolite involved in both cellular energy generation and immunomodulation, that has previously been found to promote brain health, metabolic functions, and reduce inflammation. Whether NAD+ supplementation may be beneficial for delirium has not been explored yet. In the present study, we investigate the effect of acute supplementation of NMN, a direct precursor of NAD+, in a LPS-induced acute inflammation mouse model for delirium. While NAD+ did not rescue the LPS-induced sickness behavior and metabolic dysfunction in mice, a comprehensive cytokine profile analysis did reveal rescue of plasma IFNγ levels by NMN supplementation. We also observed partial improvement on the levels of IL-12p40, LIX, and IL-17 which were sex dependent. Overall, we demonstrated that acute NAD+ boosting can lessen the LPS-induced cytokine storm in aged mice.
ARABINOSE MITIGATE METABOLIC STRESS IN INFLAMMAGING
Authors: Schmitt Brenda Landvoigt , da Pantoja Patricia Silva , Pence Brandt D 
Published: January 2025

Abstract
Arabinose (Ab) is a monosaccharide found in vegetables, fruits, and grains. It has been showed that Ab can manage blood sugar levels, improve gut health, potential anti-aging, antidepressant, antioxidants. Inflammaging is a chronic low graded inflammation commonly seen in aging, contribute significantly to age related disease. We aimed to investigate the impact of Ab on the gene expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) which are implicated in inflammaging. Additionally, we investigate if the Ab impacted the bioenergetic cell metabolism using Seahorse methodology to assess mitochondrial oxidative phosphorylation (OxPhos) activity, measured as the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) mitigating inflammation induced by lipopolysaccharide (LPS). U937 cell line was cultured in RPMI media with Pen-Strep 1% and FBS 10%. RTq-PCR and mito-stress-test using Seahorse XF technology were performed. The data analysis was performed in Prism 9. Cells were pre-treated with Ab 30 min following the addition of LPS 3h incubation time at 37°C, 5% CO2 A dose response test was performed were the pre-treatment showed significative response with Ab 50uM in pro-inflammatory cytokines genes downregulating IL-1β, IL-6, and TNF-α. The OCR metabolic phenotype and maximal respiration, under stress conditions, was improved by pre-treatment with Ab in presence of LPS. Ab protected cell to increase ECAR even in presence of LPS. Overall, these results suggest an improvement on mitochondrial OxPhos capacity maintaining the glycolysis performance of monocytic cells under inflammatory stress offering potential benefits for managing inflammaging in older populations.
AWE-INDUCTION IMPROVES RECOVERY FROM STRESS AND IMPROVES COGNITIVE PERFORMANCE
Authors: Hash Kristina , Bolling S Alison , Tower Leslie , Patrick Julie Hicks 
Published: January 2025

Abstract
Portable, affordable and nonpharmacological interventions are needed to support physical and cognitive wellbeing across the lifespan. A promising psychosocial intervention to accomplish such goals includes awe inductions. Using our previously-validated video inductions, data are provided by 61 adults (M age = 28, range 18-81 yrs). Continuous measures of heart rate variability (HRV), including respiration, Baevsky’s stress index and other indices of sympathetic and parasympathetic functioning were collected via Polar H10 heart rate monitors. Participants completed flanker and choice reaction time (RT) tasks. Manipulation checks verified adherence to the protocol. As expected, performance was slower in the cognitively challenging incongruent flanker conditions relative to the easier congruent condition. A series of moderated regressions examined whether age altered the relations between HRV and cognitive performance. Differences in Baevsky’s stress index between the Neutral and Awe conditions interacted with age to explain 27% of the variance in post-awe incongruent flanker reaction time, F (3, 51)= 6.30, p&lt;.001. Both age and the interaction term uniquely contributed to the equation. Probing the interaction revealed that although younger adults increased their speed of response under the awe condition, older adults increased their accuracy and slowed their response times accordingly. HRV indices suggested that across age, the awe induction resulted in decreased parasympathetic functioning, suggesting that awe might improve health and recovery from stress.
CANINE COGNITION ASSESSMENTS IN THE TEST OF RAPAMYCIN IN AGING DOGS (TRIAD)
Authors: Holland Sydney , Ferguson Anna , Creevy Kate , Anderson Rozalyn , Reed May 
Published: January 2025

Abstract
The primary objective of the Dog Aging Project’s Test of Rapamycin in Aging Dogs (TRIAD), is to assess whether rapamycin treatment extends lifespan in healthy, middle-aged, medium-to-large companion dogs. A secondary objective is to determine whether rapamycin impacts cognitive function in aging dogs. This placebo-controlled, double-blinded, randomized clinical trial is underway; however, baseline data allows us to investigate relationships between demographic factors and cognitive function. Dogs who met medical records eligibility criteria for TRIAD were assessed during an in-person examination by veterinary neurologists. Baseline cognitive functional assessment involved two validated clinical assessments: the Sustained Gaze test (possible scores 1 to 60 seconds; higher scores indicate greater attention span), and the CAnine DEmentia Scale (CADES) (possible scores of 0-85; higher scores indicate greater impairment). Thirty-five dogs were examined for inclusion in TRIAD, including baseline assessment of cognitive function. The median age of evaluated dogs was 9.2 years (8.1-10.2) and the median weight was 27.7 kg (24.5-30.2). The median CADES score was 1.0 (0.0-4.0) and median Sustained Gaze score was 9.0 (5.3-14.0). Post hoc analysis did not identify significant correlations between age, weight, sex, or breed and scores for either cognitive test. CADES and Sustained Gaze tests do not reveal an influence of demographic factors on cognition at middle-age in dogs being assessed for enrollment in TRIAD. Accordingly, the TRIAD clinical trial will enable robust assessment of rapamycin’s impact on age-related cognitive decline.
COMMUNITY CENTERED PERSPECTIVES ON ALZHEIMER’S DISEASE: A SYNTHESIS OF DATA FROM FOUR COMMUNITY ADVISORY BOARDS
Authors: Ahmed Ivy , DeVincentis Diane , Cronin Teresa , Pettis Jennifer 
Published: January 2025

Abstract
As the number of people with Alzheimer’s disease in the United States continues to grow, they and their caregivers face increasingly complex challenges in the health care ecosystem. To understand the impact of Alzheimer’s disease on communities, Eisai with participation of the Gerontological Society of America convened four Community Advisory Boards (CABs) inviting a variety of communities of interest across multiple disciplines that serve the community. These informative day-long meetings shed light on the local impact of this potentially devastating disease and allowed for meeting participants to share many creative solutions in place through grassroots dedication. The team identified four different communities based on their unique population and location: Dade County, Florida; Summit County, Ohio; Greater Atlanta, Georgia; Los Angeles, California. During the meetings, participants reflected on the myriad needs within their community; highlighting their most pressing unmet needs; and spotlighted successful programs, initiatives, and interactions that occur within the community to support their community members with AD and their caregivers. While each community’s needs were unique, several common themes emerged uniting these four communities illustrating shared issues and creative solutioning. Shared themes included the need for materials that address brain health to raise awareness of AD prevention and the importance of early detection, culturally tailored resources, and information delivered by trusted community champions in settings that leverage existing social outlets/meeting places. This poster will illustrate key findings from each individual meeting and highlight commonalities identified among them.
DEFINING A NOVEL HOMEOSTAT THAT SENSES THE BARRIERS OF PARENTAL REPRODUCTIVE-SPAN AND SETS PROGENY STRESS CAPACITY
Authors: Van Camp Bennett , Curran Sean 
Published: January 2025

Abstract
The ability to respond to stressors quickly and adequately is a core component to survival, and dysregulation of these resistance pathways can be debilitating. The cytoprotective transcription factor SKN-1 plays a critical role in response to multiple types of stress that can impact Caenorhabditis elegans fitness (e.g., developmental timing, reproductive output). However, activation of SKN-1 is antagonistically pleiotropic, driving multiple age-dependent pathologies. For example, as compared to wildtype animals, SKN-1 activation in early adulthood provides enhanced resistance to oxidative stress, but greater sensitivity in midlife and an early loss of motility, imbalanced lipid homeostasis and diminished lifespan. Because the negative phenotypes associated with constitutive SKN-1 activation manifest at the end of reproduction, we use long-term parental age selection of late progeny (last quartile of total progeny) as a tool to investigate the differential health of early and late progeny across the reproductive span. Selection for &gt;50 generations resulted in significant decreases of multiple SKN-1 age-related measures of health; most prominently, acute oxidative stress resistance, lipid homeostasis, movement, developmental timing, starvation resistance, and lifespan. Perhaps most surprising, acutely releasing the age selection resulted in instantaneous consequences on some, but not all, health parameters, of the immediate brood. This observation suggests the existence of a novel homeostatic set point that can bookmark the temporal boundaries of the parental reproductive span. These findings reshape the way we think about SKN-1 and the parental role in homeostatic control in offspring and provide additional details of its complex role in the regulation of healthspan.
DIETARY PROTEIN RESTRICTION IN AGED MALE MICE REDUCES MARKERS OF SASP IN ADIPOSE TISSUE
Authors: Easter Molly , Young Khristina , Sothers Hanna , Aldridge Jolaiya , Batamack Yvann , Vaddi Prerana , Morrison Christopher D , Hill Cristal 
Published: January 2025

Abstract
Aging is associated with cellular senescence and metabolic impairment of adipose tissue. Moreover, the detriments of aging are further exacerbated by obesity on metabolic function in adipose tissue. Our lab and others have shown that dietary protein restriction (DPR), without reducing calorie intake, protects against age-related metabolic decline and improves lifespan. To determine if DPR reduces cellular senescence in adipose tissue during aging and aging with obesity, 16 month old male C57BL/6 mice were fed either a normal-protein (20%), low-protein (5%), high-fat (60% fat) or a low-protein/high fat (5% protein; 60%fat) diet for 24-weeks, then adipose depots were analyzed via real time quantitative PCR (RT-qPCR) for cellular senescence markers. We found significant decreases in p21, senescence associated secretory phenotype markers(SASP) and reduced SA-Bgal staining in adipose tissue of mice that were fed a low protein diet compared to normal protein diet fed mice. Additionally, mice on a low protein diet and high fat diet demonstrated significant decreases in cellular senescence markers compared to mice on a normal protein and high fat diet, suggesting that a low protein diet decreases the burden of cellular senescence on adipose tissue in aged mice and aged obese mice. Overall, these data suggests that DPR reduces the burden of cellular senescence on adipose tissue function during aging and aging with obesity. Given the negative impact of cellular senescence on adipose tissue, DPR offers a potential dietary intervention to prevent the detriments of cellular senescence on adipose tissue function during aging and aging with obesity.
DIFFERENTIAL MICRORNAS PROFILING OF MARSHALLESE IN ARKANSAS IS ASSOCIATED WITH CHRONIC DISEASES OF AGING
Authors: Azhar Gohar , Verma Ambika , Patyal Pankaj , Riklon Sheldon , Kabua Philmar M , McElfish Pearl , Wei Jeanne Y 
Published: January 2025

Abstract
Arkansas is home to one of the largest populations from the Republic of the Marshall Islands (RMI), who have settled in the United States. Marshallese communities have a disproportionately high prevalence of many chronic diseases including diabetes, obesity, cardiovascular disease and inflammatory conditions. In the era of RNomics, miRNAs are critical in determining the cellular fate in many pathological conditions. Here we report for the first time, miRNAs expression profiling and differential expression in the Marshallese population. Blood samples were collected from 47 Marshallese subjects after obtaining informed consent and subjected to Illumina based next generation RNA sequencing. According to the sequencing reads annotation in the miRBase database, the most significantly expressed miRNAs were screened using log2 fold-change values selected with Bonferroni corrected p-value&lt; 0.01. A total of 63 human miRNAs were differentially expressed in Marshallese subjects, and among them, a total of 52 human miRNAs were significantly upregulated and 11 were significantly downregulated in males versus female subjects. Two most abundant human miRNAs families were significantly upregulated in Marshallese population including hsa-miR-548 and hsa-let-7, which play important role in regulating several inflammatory responses. Interestingly, the differentially expressed miRNAs have known associations with diabetes mellitus (hsa-miR-21, hsa-miR-26, hsa-miR-93, hsa-miR-96, hsa-miR-126), hypertension (hsa-miR-17, hsa-miR-21), Alzheimer’s disease and related dementias (AD/ADRD) (hsa-miR-144-3p, hsa-miR-144-5p) and also cardiovascular diseases (hsa-miR-548j-5p, hsa-miR-584-3p, has-let-7a-5p). Diabetes mellitus remains a major health concern in Marshallese and might accelerate co-morbidities with cardiovascular conditions and ADRD. Hence, the role of specific miRNAs associated with these conditions merits further investigation.
EVALUATING BIOLOGICAL AGE METRICS: ROBUST PREDICTORS OF AGING PROCESSES IN A CLINICAL COHORT
Authors: Stephens Elizabeth , Stubbs Brianna , Senadheera Chatura , Alexander Laura , Silverman-Martin Wendie , Peralta Sawyer , Diaz Stephanie Roa , Newman John 
Published: January 2025

Abstract
Biological age (BA) metrics, constructed by physical and biochemical markers, are increasingly recognized as powerful tools to quantify the biological processes associated with aging. Health and function in a population of the same chronological age (CA) varies between individuals. BA metrics, such as Klemera-Doubal Method (KDM), PhenoAge, and Homeostatic Dysregulation (HD) provide an age independent assessment of health status. Here, we aimed to examine the robustness and utility of BA metrics using data from a 12-week, randomized, parallel group, double-blind, placebo-controlled pilot study evaluating the safety and tolerability of a ketone ester (n=23; M=14, F=9; age=76y, range 65-90y). KDM and PhenoAge, and their residuals, showed strong correlations with CA (r=0.80 and r=0.89, respectively). In contrast, HD and its residual exhibited minimal correlation with CA (r=0.04). Using LASSO regression models, we identified several significant predictors of BA, including red cell distribution width, monocyte percentage, blood urea nitrogen, pulse and blood pressure, C-reactive protein, and total cholesterol and high-density lipoprotein. The models’ reliability was confirmed by minimizing prediction errors through cross-validated mean squared error paths, validating their robustness and generalizability. Our results validate KDM and PhenoAge as reliable BA metrics, emphasizing their potential use in clinical settings to assess aging and predict health outcomes. The biomarkers we found to predict BA reflect key biologic processes, such as inflammation, immune, kidney, and cardiac function, and metabolism, underscoring the relevance of BA as a complement to CA in assessing aging and age-related health risks.
HOW CAREGIVING SHAPES THE RELATIONSHIP BETWEEN ACTIVITY DIVERSITY AND COGNITION
Authors: Doyle Cassidy , Small Brent 
Published: January 2025

Abstract
Research indicates frequent participation in leisure activities may buffer age-related cognitive declines but activity variety (activity diversity) may have a stronger relationship with cognitive health. The activity diversity-cognition relationship is understudied in caregivers, who may be susceptible to cognitive declines and therefore benefit from leisure activity participation. This study examines cognitive differences between caregivers and non-caregivers and explores the roles of activity diversity and frequency in this relationship. The Health and Retirement Study was used to investigate the relationship between activity diversity, activity frequency, and cognition among caregivers (N=6,472) and non-caregivers (N=4,343). From the Psychosocial and Lifestyle Questionnaire, 17 leisure activities were assed to create activity diversity and frequency measures. Activity diversity was quantified using Shannon’s entropy, scores ranging from 0 to 1, with higher scores indicating greater diversity. Activity frequency was measured by summing the frequencies of 17 activities (0=not at all to 5=daily). Moderation analysis assessed the relationships between activity diversity-cognition and activity frequency-cognition by caregiving status. The sample was predominantly female, White, educated, and engaged in diverse activities (M=0.49). Caregivers had lower cognition than non-caregivers (p &lt;.001). Moderation analyses showed that higher activity diversity and frequency were associated with better cognition for both groups, with stronger effects for non-caregivers (p &lt;.001). Greater leisure frequency and diversity were related to better cognition. Unexpectedly, caregivers engaged in more frequent and diverse leisure compared to caregivers, but associations between activity engagement-cognition were stronger in non-caregivers. Investigating the potential for activity engagement to benefit multiple domains (wellbeing, stress, cognition) among caregivers is warranted.