bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 screening in patients in need of urgent inpatient treatment in the Emergency Department (ED) by digitally integrated point-of-care PCR: A clinical cohort study
Authors: Möckel Martin , Bolanaki Myrto , Hofmann Jörg , Stein Angela , Hitzek Jennifer , Holert Fabian , Fischer-Rosinský Antje , Slagman Anna 
Published: January 2022

AbstractPatients in need of urgent inpatient treatment were recruited prospectively. A rapid point of care PCR test (POC-PCR; Liat®) for SARS-CoV2 was conducted in the ED and a second PCR-test from the same swab was ordered in the central laboratory (CL-PCR). POC-PCR analyzers were digitally integrated in the laboratory information system.Overall, 160 ED patients were included. A valid POC-PCR-test result was available in 96.3% (n=154) of patients. N=16 patients tested positive for SARS-CoV-2 (10.0%). The POC PCR test results were available within 102 minutes (median, IQR: 56-211), which was significantly earlier compared to the CL PCR (811 minutes; IQR: 533-1289, p < 0.001). The diagnostic accuracy of the POC-PCR test was 100%. The implementation and digital LIS integration was successfully done. Staff satisfaction with the POC process was high.The POC-PCR testing in the emergency department is feasible and shows a very high diagnostic performance.Trial registration: DRKS00019207
Up-regulated tumor intrinsic growth potential and decreased immune function orchestrate the evolution of lung adenocarcinoma
Authors: Zhao Yue , Shang Jun , Gao Jian , Han Han , Gao Zhendong , Yan Yueren , Zheng Qiang , Ye Ting , Fu Fangqiu , Deng Chaoqiang , Ma Zelin , Zhang Yang , Zheng Difan , Zheng Shanbo , Li Yuan , Cao Zhiwei , Shi Leming , Chen Haiquan 
Published: January 2022

AbstractIntroductionLung adenocarcinoma is the most common pathological subtype of lung cancer. Precursors of lung adenocarcinoma, namely adenocarcinoma in situ and minimally invasive adenocarcinoma, have a superb 5-year survival rate after surgical resection. A deeper understanding of the key genetic changes driving the progression of lung adenocarcinoma is needed.MethodsIn this study, we performed whole-exome sequencing and RNA-sequencing on surgically resected 24 AIS, 74 MIA, 99 LUAD specimens and their adjacent paired normal tissues. Radiological, clinical, and pathological characteristics were recorded. Gene expression patterns were identified to find key pathways driving the progression of lung adenocarcinoma. Furthermore, genomic alterations and differential expression analyses were performed to compare tumors with different radiological manifestations. Finally, a progressive index was developed to quantitatively measure the level of imbalance between tumor intrinsic growth potential and immune microenvironment.Results12 patterns of gene expression were identified. Pathways associated with tumor growth and metastasis were found to be up-regulated as tumors progressed, while pathways associated with immune function were found to be down-regulated. Deconvolution of RNA-seq data also showed a decrease of CD8+ T cells and an increase of Tregs as the tumors progressed. Furthermore, tumors with more solid components on CT scan had a higher mutation frequency of tumor suppressor genes, higher tumor mutation burden and higher frequency of somatic copy number alterations. Finally, tumor progressive index demonstrated an increasing trend with the progression of lung adenocarcinoma.DiscussionOur results reveal the imbalance of tumor intrinsic factors and immune function orchestrate the progression of lung adenocarcinoma.
Use of Andrographis paniculata (Burm.f.) Wall. ex Nees and risk of pneumonia in hospitalised patients with mild COVID-19: a retrospective cohort study
Authors: Tanwettiyanont Jeeranan , Piriyachananusorn Napacha , Sangsoi Lilit , Boonsong Benjawan , Sunpapoa Chamlong , Tanamatayarat Patcharawan , Na-Ek Nat , Kanchanasurakit Sukrit 
Published: January 2022

AbstractBackgroundAndrographis paniculata (Burm.f.) Wall. ex Nees (AP) has been widely used in Thailand to treat mild COVID-19 infections since early 2020; however, supporting evidence is scarce and ambiguous. Thus, this study aimed to examine whether the use of AP is associated with a decreased risk of pneumonia in hospitalised mild COVID-19 patients.MethodsWe collected data between March 2020 and August 2021 from COVID-19 patients admitted to one hospital in Thailand. Patients whose infection was confirmed by real-time polymerase chain reaction, had normal chest radiography and did not receive favipiravir at admission were included and categorised as either AP (deriving from a dried and ground aerial part of the plant), given as capsules with a total daily dose of 180 mg of andrographolide for five days or standard of care. They were followed for pneumonia confirmed by chest radiography. Multiple logistic regression was used for the analysis controlling for age, sex, diabetes, hypertension, statin use, and antihypertensive drug use.ResultsA total of 605 out of 1,054 patients (mostly unvaccinated) were included in the analysis. Of these, 59 patients (9.8%) developed pneumonia during the median follow-up of 7 days. The incidence rates of pneumonia were 13.93 (95% CI 10.09, 19.23) and 12.47 (95% CI 8.21, 18.94) per 1,000 person-days in the AP and standard of care groups, respectively. Compared to the standard of care group, the odds ratios of having pneumonia in the AP group were 1.24 (95% CI 0.71, 2.16; unadjusted model) and 1.42 (95% CI 0.79, 2.55; fully adjusted model). All sensitivity analyses were consistent with the main results.ConclusionsThe use of AP was not significantly associated with a decreased risk of pneumonia in mild COVID-19 patients. While waiting for insights from ongoing trials, AP’s use in COVID-19 should be done with caution.
The NILS study protocol - a retrospective validation study of a preoperative decision-making tool for non-invasive lymph node staging in women with primary breast cancer [ISRCTN14341750]
Authors: Skarping Ida , Dihge Looket , Bendahl Pär-Ola , Huss Linnea , Ellbrant Julia , Ohlsson Mattias , Rydén Lisa 
Published: January 2022

AbstractBackgroundRoutine preoperative axillary ultrasonography has proven insufficient for detecting low-burden nodal metastatic deposits. For the majority of newly diagnosed breast cancer patients presenting with clinical T1-T2 N0 disease, the standard axillary staging by sentinel lymph node biopsy is not therapeutic. The pilot non-invasive lymph node staging (NILS) artificial neural network (ANN) model to predict nodal status was published in 2019. The aim of the current study is to assess the performance measures of the model for the prediction of healthy lymph nodes in clinically N0 breast cancer patients at two breast cancer centers in Sweden.MethodsThis bicenter, observational, retrospective study has been designed to validate the NILS prediction model for nodal status using preoperatively collected clinicopathological and radiological data. A web-based implementation of the nodal status classifier has been developed and will be used in this study, resulting in an estimated probability of healthy lymph nodes for each study participant. Our primary endpoint is to report on the performance of the NILS prediction model to distinguish between healthy and metastatic lymph nodes (discrimination, N0 vs. N+) and compare the observed and predicted event rates of benign axillary nodal status (calibration).DiscussionInternationally, there are numerous artificial intelligence projects involving non-invasive identification of N0 breast cancer. Here, we present a robust validation study based on external cohorts of our ANN model. Although validation is necessary to show generalizability, it is often overlooked. If the accuracy and discrimination reach a satisfactory level, our prediction tool can be implemented to assist medical professionals and breast cancer patients in shared decision-making on omitting sentinel node biopsy in patients predicted to be node-negative. In future, this may potentially save healthcare resources and reduce costs and adverse side effects. In addition, our study might prompt future studies of nodal metastases of malignancies in other organs, and thus might have implications beyond breast cancer.Trial registrationThis study has been prospectively registered in the ISRCTN registry, identification number: 14341750
Large household reduces dementia mortality
Authors: You Wenpeng , Henneberg Maciej 
Published: January 2022

AbstractBackgroundLarge households/families create more positive psychological well-being which may offer a life course protection against dementia development and deliver more comprehensive healthcare to dementia patients.MethodsDementia specific mortality rates of the 183 member states of World Health Organization were calculated and matched with the respective country data on household size, Gross Domestic Product (GDP), urban and ageing. Scatter plots were produced to explore and visualize the correlations between household size and dementia mortality rates. Pearson’s and nonparametric correlations were used to evaluate the strength and direction of the associations between household size and all other variables. Partial correlation of Pearson’s moment-product approach was used to identify that household size protects against dementia regardless of the competing effects from ageing, GDP and urbanization. Multiple regression identified large household was a significant predictor of dementia mortality.ResultsHousehold size was in a negative and moderately strong correlation (r = -0.6034, p < 0.001) with dementia mortality. This relationship was confirmed in both Pearson r (r= - 0.524, p<0.001) and nonparametric (rho□=□-0.579, p□<□0.001) analyses. Regardless of the contribution of ageing, SES and urban lifestyle to dementia mortality, large household was an independent predictor of dementia mortality (r = -0.331, p <0.001) in partial correlation analysis. Stepwise multiple regression analysis selected large household as the variable having the greatest contribution to dementia mortality with R2 = 0.263 while ageing was placed second increasing R2 to 0.259. GDP and urbanization were removed as having no statistically significant influence on dementia mortality.ConclusionsIndependent of ageing, urbanization and GDP, large household protects against dementia mortality. As part of dementia prevention, healthcare practitioners should encourage people to increase their positive interactions with persons from their neighbourhood or other fields where large household/family size is hard to achieve.
Cognitive predictors of vaccine hesitancy and COVID-19 mitigation behaviors in a population representative sample
Authors: Hudson Anna , Hall Peter A. , Hitchman Sara C. , Meng Gang , Fong Geoffrey T. 
Published: January 2022

AbstractWith the continued threat of COVID-19, predictors of vaccination hesitancy and mitigation behaviors are critical to identify. Prior studies have found that cognitive factors are associated with some COVID-19 mitigation behaviors, but few studies employ representative samples and to our knowledge no prior studies have examined cognitive predictors of vaccine hesitancy. The purpose of the present study, conducted among a large national sample of Canadian adults, was to examine associations between cognitive variables (executive function, delay discounting, and temporal orientation) and COVID-19 mitigation behaviors (vaccination, mask wearing, social distancing, and hand hygiene). Findings revealed that individuals with few executive function deficits, limited delay discounting and who adopted a generally future-orientation mindset were more likely to be double-vaccinated and to report performing COVID-19 mitigation behaviors with high consistency. The most reliable findings were for delay discounting and future orientation, with executive function deficits predicting mask wearing and hand hygiene behaviors but not distancing and vaccination. These findings identify candidate mediators and moderators for health communication messages targeting COVID-19 mitigation behaviors and vaccine hesitancy.
Optogenetic Manipulation of Cell Migration with High Spatiotemporal Resolution Using Lattice Lightsheet Microscopy
Authors: Tang Wei-Chun , Liu Yen-Ting , Yeh Cheng-Han , Lin Yi-Ling , Lin Yu-Chun , Hsu Tsui-Ling , Gao Liang , Chang Shu-Wei , Chen Peilin , Chen Bi-Chang 
Published: January 2022

AbstractLattice lightsheet microscopy (LLSM) is modified with the aim of manipulating cellular behavior with subcellular resolution through three-dimensional (3D) optogenetic activation. In this study, we report a straightforward implementation of the activation source in LLSM in which the stimulating light can be generated by changing the spatial light modulator (SLM) patterns and the annual masks. As a result, a Bessel beam as a stimulation source is integrated into the LLSM without changing the optical configuration, achieving better spatiotemporal control of photoactivation. We show that the energy power required for optogenetic reactions is lower than 1 nW (or 24 mW/cm2), and membrane ruffling can be activated at different locations within a cell with subcellular resolution. We also demonstrate guided cell migration using optogenetic stimulation for up to 6 h, where 463 imaging volumes are collected, without noticeable damage to cells.
The Layer 7 Cortical Interface: A Scalable and Minimally Invasive Brain–Computer Interface Platform
Authors: Hettick Mark , Ho Elton , Poole Adam J. , Monge Manuel , Papageorgiou Demetrios , Takahashi Kazutaka , LaMarca Morgan , Trietsch Daniel , Reed Kyle , Murphy Mark , Rider Stephanie , Gelman Kate R. , Byun Yoon Woo , Hanson Timothy , Tolosa Vanessa , Lee Sang-Ho , Bhatia Sanjay , Konrad Peter E. , Mager Michael , Mermel Craig H. , Rapoport Benjamin I. 
Published: January 2022

AbstractProgress toward the development of brain–computer interfaces has signaled the potential to restore, replace, and augment lost or impaired neurological function in a variety of disease states. Existing approaches to developing high-bandwidth brain–computer interfaces rely on invasive surgical procedures or brain-penetrating electrodes, which limit addressable applications of the technology and the number of eligible patients. Here we describe a novel approach to constructing a neural interface, comprising conformable thin-film electrode arrays and a minimally invasive surgical delivery system that together facilitate bidirectional communication with large portions of the cortical surface (enabling both recording and stimulation). We demonstrate the feasibility and safety of delivering reversible implants containing over 2,000 microelectrodes to multiple functional regions in both hemispheres of the brain simultaneously, without requiring a craniotomy or damaging the cortical surface, at an effective insertion rate faster than 40 ms per channel. We further evaluate the performance of this system immediately following implantation for high-density neural recording and visualizing cortical surface activity at spatial and temporal resolutions and extents not previously possible in multiple preclinical large animal studies as well as in a five-patient pilot clinical study involving both anesthetized and awake neurosurgical patients. We characterize the spatial scales at which sensorimotor activity and speech are represented at the cortical surface, demonstrate accurate neural decoding of somatosensory, visual, and volitional walking activity, and achieve precise neuromodulation through cortical stimulation at sub-millimeter scales. The resulting system generates 90 Gb/h of electrophysiologic data, and demonstrates the highly scalable nature of micro-electrocorticography and its utility for next-generation brain-computer interfaces that may expand the patient population that could benefit from neural interface technology.
Retrieval context determines whether event boundaries impair or enhance temporal order memory
Authors: Wen Tanya , Egner Tobias 
Published: January 2022

AbstractMeaningful changes in context create “event boundaries”, segmenting continuous experience into distinct episodes in memory. A foundational finding in this literature is that event boundaries impair memory for the temporal order of stimuli spanning a boundary compared to equally spaced stimuli within an event. This seems surprising in light of intuitions about memory in everyday life, where the order of within-event experiences (did I have coffee before the first bite of bagel?) often seems more difficult to recall than the order of events per se (did I have breakfast or do the dishes first?). Here, we aimed to resolve this discrepancy by manipulating whether stimuli carried information about their encoding context during retrieval, as they often do in everyday life (e.g., bagel-breakfast). In Experiments 1 and 2, we show that stimuli inherently associated with a unique encoding context produce a “flipped” order memory effect, whereby temporal memory was superior for cross-boundary than within-event item pairs. In Experiments 3 and 4, we added context information at retrieval to a standard laboratory event memory protocol where stimuli were encoded in the presence of arbitrary context cues (colored frames). We found that whether temporal order memory for cross-boundary stimuli was enhanced or impaired relative to within-event items depended on whether the context was present or absent during the memory test. Taken together, we demonstrate that the effect of event boundaries on temporal memory is malleable, and determined by the availability of context information at retrieval.
Intestinal Mucin Is a Chaperone of Multivalent Copper
Authors: Reznik Nava , Gallo Annastassia D. , Rush Katherine W. , Javitt Gabriel , Fridmann-Sirkis Yael , Ilani Tal , Nairner Noa A. , Chacón Kelly N. , Franz Katherine J. , Fass Deborah 
Published: January 2022

SUMMARYMucus protects the body by many mechanisms, but a role in managing toxic transition metals was not previously known. Here we report that secreted mucins, the major mucus glycoproteins coating the respiratory and intestinal epithelia, are specific copper-binding proteins. Most remarkably, the intestinal mucin, MUC2, has two juxtaposed copper binding sites, one that accommodates Cu2+and the other Cu1+, which can be formedin situby reduction with vitamin C. Copper is an essential trace metal because it is a cofactor for a variety of enzymes catalyzing electron transfer reactions, but copper damages macromolecules when unregulated. We observed that MUC2 protects against copper toxicity while permitting nutritional uptake into cells. These findings introduce mucins, produced in massive quantities to guard extensive mucosal surfaces, as extracellular copper chaperones and potentially important players in physiological copper homeostasis.
Determinants of entrepreneurial alertness: towards sustainable agribusiness development
Authors: Shiri Nematollah , Mehdizadeh Hossein , Khoshmaram Mojgan , Azadi Hossein 
Published: January 2022

PurposeEntrepreneurship is known to be important to the economy, and many scholars across the globe have researched it from a number of viewpoints. Currently, there is a need for an academic study to explore this area by combining sustainability value creating practices and the efforts of current entrepreneurs towards the said target, particularly in the case of the agricultural sector. While the entrepreneurship studies have mostly focused on the determinants of entrepreneurial opportunity recognition, few studies have attempted to analyze the factors influencing the entrepreneurial alertness (EA) of students, especially in relation to agricultural students. To fill this gap, this work investigated the impact of human and social capital on EA among the students of agricultural higher education in Iran.Design/methodology/approachThe sample consisted of 254 agricultural students in higher education from Ilam province in the Islamic Republic of Iran, selected by the stratified random sampling method for the study. Modeling of structural equations was used in inferential statistics.FindingsAccording to the results of the trial, human resources and social capital (SC) have been seen to have a strong, optimistic and measurable impact on EA. Key findings also show that human capital (HC) has an indirect, optimistic and important effect on EA through the mediator role of SC. Establishing higher education science teams, groups, networks and associations can foster opportunities to create and develop relationships and communication between agricultural students and entrepreneurs.Originality/valueThese findings illustrate the value of human and social resources in fostering entrepreneurship alertness among Iranian students of agricultural higher education. Considering the research results, the authors recommend some theoretical and realistic implications and suggestions for ways of promoting and increasing EA among farm students to encourage sustainable growth of agricultural careers in western Iran.
The emotional cost-of-carry: Chinese investor sentiment and equity index futures basis
Authors: Cao Song , Li Ziran , Koedijk Kees G. , Gao Xiang 
Published: January 2022

PurposeWhile the classic futures pricing tool works well for capital markets that are less affected by sentiment, it needs further modification in China's case as retail investors constitute a large portion of the Chinese stock market participants. Their expectations of the rate of return are prone to emotional swings. This paper, therefore, explores the role of investor sentiment in explaining futures basis changes via the channel of implied discount rates.Design/methodology/approachUsing Chinese equity market data from 2010 to 2019, the authors augment the cost-of-carry model for pricing stock index futures by incorporating the investor sentiment factor. This design allows us to estimate the basis in a better way that reflects the relationship between the underlying index price and its futures price.FindingsThe authors find strong evidence that the measure of Chinese investor sentiment drives the abnormal fluctuations in the basis of China's stock index futures. Moreover, this driving force turns out to be much less prominent for large-cap stocks, liquid contracting frequencies, regulatory loosening periods and mature markets, further verifying the sentiment argument for basis mispricing.Originality/valueThis study contributes to the literature by relying on investor sentiment measures to explain the persistent discount anomaly of index futures basis in China. This finding is of great importance for Chinese investors with the intention to implement arbitrage, hedging and speculation strategies.