bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA PEBP1P2 Suppresses the Progression of Clear Cell Renal Cell Carcinoma Cells by Regulating the miR-27a-3p/PEBP1 Signal
Authors: Mu Jiao , Tang Jianying , Su Yuanyuan , Yang Lu , Wang Jing 
Published: March 2022

Abstract
Background: Long noncoding RNA (lncRNA) PEBP1P2 has been shown to suppress the proliferation of vascular smooth muscle cells, but its role and underlying mechanisms in the development of clear cell renal cell carcinoma (ccRCC) remain largely unclear. Methods: Gene expression profiling interactive analysis (GEPIA) database was used to analyze the aberrant expression of lncRNAs in ccRCC tissues. Expression of RNAs and proteins was detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blotting, respectively. Ectopic expression of PEBP1P2 and miR-27a-3p was performed by pcDNA3.1 vector and miR-27a-3p mimics, respectively. Knockdown of PEBP1 mRNA was conducted by PEBP1 siRNA. Cell proliferation, migration, and invasion were analyzed using a CCK8 reagent and a trans-well assay, respectively. The sponge of PEBP1P2 to miR-27a-3p was investigated using Luciferase report system and RNA pull-down assays. Results: PEBP1P2 expression was decreased in ccRCC tissues compared with that in normal tissues and correlated with the size, TNM stage, and lymph node metastasis of the tumors, as well as the poor survival rates of ccRCC patients. Functionally, the enforced expression of PEBP1P2 inhibited the ability of ccRCC cells to proliferate, migrate, and invade in vitro . Mechanistically, PEBP1P2 suppressed the biological functions of ccRCC cells via increasing the mRNA and protein expression of PEBP1 and their expression had a positive correlation in ccRCC tissues. Furthermore, PEBP1P2 upregulated PEBP1 expression through acting as a competing endogenous RNA (ceRNA) to sponge miR-27a-3p, which directly target PEBP1 mRNA 3’UTR. Moreover, miR-27a-3p mimics rescued the decrease in the ability of ccRCC cells to proliferate, migrate, and invade mediated by PEBP1P2. Conclusion: Our findings indicated that PEBP1P2 suppressed ccRCC cell proliferation via regulating the miR-27a-3p/PEBP1 signal, suggesting that the PEBP1P2-miR-27a-3p/PEBP1 axis may be a potential therapeutic target for ccRCC.
Acute exposure of minimally ox-LDL elicits survival responses by downregulating the mediators of NLRP3 inflammasome in cultured RAW 264.7 macrophages
Authors: Thankam Finosh , Khwaja Bisma , Nguyen Megan , Ahsan Osama , Agrawal Devendra 
Published: March 2022

Abstract
Lipid burden in macrophages driven by oxidized LDL (oxLDL) accelerates the foam cell formation and the activation of sterile inflammatory responses aggravating the atherosclerosis. However, there is limited information on the mediators and the pathways involved in the possible survival responses, especially at the initial phase, by lipid-burden in macrophage cells on encountering oxLDL. Hence, the present study was designed to assess the expression status of major mediators involved in the NLRP3 inflammasome pathway of sterile inflammation and the cellular responses in oxLDL-challenged cultured RAW 264.7 macrophage cells. The ox-LDL-treated RAW 264.7 macrophage cells displayed a decreased expression of the key sterile inflammatory mediators, TLR4, TLR2, ASC, NLRP3 and IL-18 at the protein and transcript level; however, displayed increased level of IL-1β, RAGE and TREM1 at the protein level. The biological responses including lipid uptake, lipid peroxidation, superoxide production and mitochondrial membrane potential were significantly decreased in oxLDL-treated macrophages. Moreover, cellular hypertrophy and mitochondrial density were significantly decreased in the oxLDL-treated macrophages compared to the control. Overall, the findings revealed that the expression status of key sterile mediators and the macrophage response during the initial phase of oxLDL exposure tend toward the prevention of inflammation. Further understanding would open novel translational opportunities in the management of atherosclerosis.
A novel defined necroptosis-associated gene signature for predicting the prognosis of hepatocellular carcinoma
Authors: Li Yiming , Chen Wenxiang , Wang Yu , He Jun , Zhou Jianyin 
Published: March 2022

Abstract
Background
Hepatocellular carcinoma (HCC) is a highly heterogeneous disease with a poor prognosis and insignificant immunotherapeutic effects. This project attempted to identify new potential biomarkers for necroptosis-associated genes to predict prognosis and improve the situation.
Method
The mRNA expression profiles of HCC patients and their clinical data were downloaded from public databases. Finding differential signature genes (DSGs) after subtyping patients according to the necroptosis-related genes. Gene set enrichment analysis was used to conduct GO terms and KEGG pathways. DSGs were analyzed in TCGA cohort to build a predictive model. Validation was performed on HCC patients from ICGC cohort. The model was evaluated using Kaplan–Meier analysis, time-dependent receiver operating characteristics (ROC), uni-Cox regression, and multi-Cox regression. The immune cell infiltration score and related pathway activity were calculated using single-sample gene set enrichment analysis.
Result
A signature model of necroptosis-associated genes was created. The low-risk group had a more prolonged OS when different risk subgroups were compared. The prognostic model's predictive ability was confirmed by ROC analysis. The risk score was an independent predictor of OS in a multi-Cox analysis. According to functional analysis, immune status differed between the two risk groups. The risk score was significantly related to tumor grade, tumor stage, and immune infiltrate type.
Conclusion
A novel signature made up of four necroptosis-associated genes can be used to predict prognosis and influence immune status in HCC. Furthermore, these genes could be used as immunotherapy targets.
High Intensity Interval Training Is More Beneficial to Regulate Free Radicals and mtDNA Oxidative Damage in Aged Rats.
Authors: Askarian Ali , Kordi Mohammadreza , Choobineh Siroos , Gaeini AbbasAli 
Published: March 2022

Abstract
Background: The aim of this study was to compare the effect of 12 weeks of high intensity interval training (HIIT) and continues training (CT) on SIRT3 protein level, FOXO3 gene expression, and mtDNA oxidative damage in gastrocnemius of female aged rats. Methods and Results: Female wistar rats (18 months, 250-300 g, n=36) were divided into three groups (HIIT, CT, control). Based on aerobic capacity determined using the ramp protocol, HIIT intensity corresponded to 85-90% and CT was 65-70% of VO2max. Rats trained three times per week for 12 weeks. Forty-eight hours after the last training session, rats were sacrificed and gastrocnemius muscle excised. Our results showed that both exercise protocol increased SIRT3 protein content and FOXO3 gene expression compared to control. However, no significant difference was observed between HIIT and CT. In addition, our data showed that MnSOD activity was significantly higher in HIIT compared to both CT and control and CT versus control. Finally, the level of 8-OHdg was significantly lower in HIIT and CT compered to control and there was no significant difference between HIIT and CT. Conclusion: Our results showed that 12 weeks of HIIT and CT increase SIRT3 protein content, improve antioxidant defense of aged female waster rats. Therfore, HIIT is a time efficient protocol for improving some aspects of mitochondrial function.
Cerebral Processing of Emotions in Phantom Eye Pain Patients: An Event Related Potential Study
Authors: Lou Lixia , Wang Yijie , Zhang Bingren , Jia Yanli , Wang Wei , Ye Juan 
Published: March 2022

Abstract
Background: Phantom eye pain (PEP) is a major clinical problem after eye removal with no standard treatment protocol to date. As pain is a multidimensional experience associated with emotional and cognitive components, this study aimed to explore the possible neuropsychological mechanisms of PEP in a perspective of emotional cognition, in order to provide a basis for clinical treatment.Methods: Visual oddball event-related potentials (ERPs) under different external emotional stimuli (Disgust, Fear, Sadness, Happiness, Erotica and Neutral) were tested in 12 patients and 12 healthy volunteers. Participants’ affective states were measured with the Mood Disorder Questionnaire (MDQ), the Hypomania Checklist-32 (HCL-32), and the Plutchik–van Praag Depression Inventory (PVP). The amplitudes and latencies of N1, P2, N2 and P3 components were analyzed by three-way ANOVA, i.e., group (2) × emotion (6) × electrode (3). Multiple comparisons were performed using Bonferroni’s test.Results: Longer N1 latencies, increased N1 amplitudes; shorter P2 latencies under Disgust and Happiness, decreased P2 amplitudes; shorter N2 latencies under Erotica, increased N2 amplitudes were found in patients compared with controls. There was no main effect of group or interaction effect on P3 latencies and P3 amplitudes. The MDQ and HCL-32 scores were lower, and the N1 latencies under Sadness were negatively correlated with PVP scores in patients.Conclusions: PEP patients showed reversed patterns in exogenous attention allocation and enhanced involuntary attention to emotional stimuli compared with controls. This study demonstrated cortical processing of emotions in PEP patients and could provide a basis for developing emotional intervention therapy.
Pasteurella multocidia toxin aggravates ligatured-induced periodontal bone loss and inflammation via NOD-like receptor protein 3 inflammasome
Authors: Han Yineng , Gao Pengfei , Yang Qiaolin , Huang Yiping , Jia Lingfei , Zheng Yunfei , Li Weiran 
Published: March 2022

Abstract
NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome is reportedly involved in periodontal pathogenesis. Pasteurella multocida toxin (PMT) is the major virulence factor of Pasteurella multocida strains, which belongs to the GNFR. The existence of GNFR and their toxin may aggravate periodontitis. Therefore, it’s important to unclose the regulatory mechanisms of PMT in periodontitis. However, the involvement of NLRP3 inflammasome and PMT in periodontitis remain unclear. The results showed that NLRP3 expression was increased in human and mice gingivae from periodontitis patients and periodontitis mice by immunohistochemical staining and quantitative reverse transcription polymerase chain reaction (qRT-PCR). Nlrp3-/- mice showed less periodontal bone loss and lower abundances of Pasteurella multocida by 16S rRNA sequencing. PMT promoted NLRP3 expressions by activating nuclear factor kappa light chain enhancer of B cells (NF-κB) pathway and activated NLRP3 inflammasome. This effect was reversed by NLRP3 inhibitor MCC950. Furthermore, PMT aggravated periodontal bone loss and inflammation in WT mice while MCC950 attenuated periodontal bone loss and inflammation. The Nlrp3-/- periodontitis models with PMT local injection showed less bone loss and inflammation compared with WT periodontitis mice after PMT treatment. Taken together, our results showed that PMT aggravates periodontal response to the ligature by promoting NLRP3 expression and activating NLRP3 inflammasome, suggesting that NLRP3 may be an effective target for the treatment of periodontitis caused by GNFR and MCC950 may be a potential drug against this disease.
Asymptomatic Mesh Infection 6 Years After Laparoscopic Totally Extraperitoneal Inguinal Hernia Repair: a Rare Case Report
Authors: Li Zhiwang , Zeng Haijing , Liu Yuping , Wu Zuguang , Li En 
Published: March 2022

Abstract
Background: Totally extraperitoneal(TEP) inguinal hernia repair is a minimally invasive procedure. Mesh infection is one of the challenging complications after TEP inguinal hernia repair. However, the cases of delayed asymptomatic mesh infection caused by hernia mesh is extremely rare. This report aims to reveal that the risk of mesh infection may occur after a long period of this surgery. This report is also intended to suggest surgeons pay more attention to the follow-up of such patients and to be aware of the possibility of asymptomatic mesh infection to assist in early diagnosis and treatment.Case presentation: A 63-year-old male patient, who underwent TEP inguinal hernia repair 6 years ago for right inguinal hernia, fell down accidentally 2 weeks ago. Enhanced computed tomography(CT) showed lower right abdomen space-occupying lesions. So he underwent laparoscopic surgery during which abscess caused by delayed mesh infection was found. After removing the mesh and abscess, he was discharged.Conclusion: The risk of mesh infection after TEP inguinal hernia repair is low, but it can last for more than 6 years and can even be asymptomatic as long as the mesh remains in the body.
Difficult Airway Management in a Patient With Upper Airway Obstruction Due to Alkali Ingestion: a Case Report
Authors: Taguchi Azusa , Fukaguchi Kiyomitsu , Fukui Hiroyuki , Sekine Ichiro , Yamagami Hiroshi 
Published: March 2022

Abstract
Background: Ingestion of alkaline substances can cause mucosal damage and upper airway obstruction. This is a serious and potentially fatal complication that occurs within the first few hours after ingestion. There has been no established algorithm for emergent airway management in these situations. We present a case of difficult airway management in a patient with upper airway obstruction caused by alkali ingestion, wherein we opted for intubation with video laryngoscopy and successfully treated the patient.Case presentation: An 80-year-old patient accidentally ingested a small amount of alkaline liquid and presented to our hospital with complaints of burning sensation in the throat. Within two hours of arrival at the hospital, the edema in the epiglottis rapidly worsened, resulting in upper airway obstruction. Assuming that intubation and ventilation would be difficult in this situation, we attempted to intubate the patient using fiberoptic bronchoscopy, but it was difficult. Finally, after two unsuccessful attempts, we successfully intubated the patient using a video laryngoscope.Conclusions: The patient in our case had acute upper airway obstruction due to alkali ingestion, which caused difficulty in tracheal intubation. Our experience, in this case, would likely help emergency physicians to intubate more safely in patients with emergent upper airway obstruction due to alkali ingestion. For intubation in such situations, it would be a reasonable option to use video laryngoscopy.
Photo-screening for Retinoblastoma: A Proof-of-Concept Study
Authors: Vempuluru Vijitha S , Jonnadula Ganesh Babu , Bharadwaj Shrikant , Kaliki Swathi 
Published: March 2022

Abstract
Purpose: The potential of Infrared (IR) photo-screening for detection of retinoblastoma has not been assessed. This study aimed to assess the IR reflex formed across the pupil in eyes with retinoblastoma (RB), vis-à-vis eyes with no intraocular tumor.Methods: Central gaze IR-reflex assessment in eyes with and without RB.Results: Of 30 eyes from 21 subjects, 20 eyes had RB and 10 eyes had no tumors. Image frames were extracted from IR video recordings of 30 eyes, of which 71 central gaze images were eligible for measurement of pixel intensity (0 to 255). Of these, 49 images had RB and 22 images had no tumor. The mean pixel intensity (MPI) within the pupil, in images of eyes with RB was 115 (median, 92; range, 38 to 254), in images of eyes with no tumor was 73 (median, 68; range, 26 to 125) and the difference was statistically significant (Odds ratio 1.018; 95 % CI: 1.004 – 1.032; p, .010;) on bivariate analysis. Of 57 images from eyes with RB, 35 were captured with un-dilated pupils and 22 with dilated pupils. The MPI in images from un-dilated eyes with RB was 107 (median, 79; range, 38 to 254), in images from dilated eyes with RB was 128 (median, 114; range, 46 to 215). However, the difference was not statistically significant (Odds ratio 1.006; 95 % CI: 0.996– 1.015; p, .248).Conclusion: Technology combining IR imaging and MPI measurement within the pupil can translate into a potential tool for early detection of RB.
A new heuristics algorithm for solving MAX-SPA-P problem of large sizes
Authors: Nguyen Giang L. , Thi Uyen Nguyen , Tran Sang X. , Huu Viet Hoang 
Published: March 2022

Abstract
The purpose of the MAX-SPA-P problem is to establish stable pairs between students and the projects so that the number of matched students is maximum. The MAX-SPA-P problem has been attracting research attention because the theory of stable matching in the MAX-SPA-P problem is widely applied in the fields of education and economics such as finding matching between students and universities, buyers and sellers, suppliers and carriers, etc or any matching problems which have preference list constraints and the maximum number of matchings. So far, several approaches are proposed to solve this problem such as approximation algorithm, min-conflict search, and local search. However, those approaches still have drawbacks in computation time and solution quality for solving the MAX-SPA-P problem of large sizes. This paper presents a new heuristic search to improve performance to solve the MAX-SPA-P problem of large sizes. Our proposed method is to start from a blank matching, we find a maximum stable matching based on Gale-Shapely’s idea by assigning active students to projects which they prefer most on their rank list. If a project or lecturer is over-subscribed, we define a heuristic function for selecting a suitable student to remove from current matching. This assigning process will stop when all students are inactive. The experimental outcomes confirm the better efficiency of our algorithm than both SPA-P-AP and SPA-P-MCH ones based on computation time and solution quality for solving MAX-SPA-P problem of large sizes.
Blastocystis is widely distributed in mammals and aves
Authors: Liu Liwen , Sun Hanzhi , Wang Yuwei , Feng Gang , Zhang Yongbin , Cao Mengjuan , Lu Xin , Ju Huidong , Zhang Luping , Ma Lei 
Published: March 2022

Abstract
Background: Blastocystis sp. is a unicellular protozoan and one of the most common gastrointestinal eukaryotic parasites in humans and animals. It is distributed worldwide, but its pathogenic role is still controversial. Blastocystis sp. is subdivided into 33 subtypes, and existing different subtypes imply host specificity in various species. The study aimed to investigate in detail the host range of Blastocystis sp., given the limited studies. Methods: Many fresh fecal samples were randomly collected from zoo animals and tested by polymerase chain reaction (PCR) assay. Sequence alignment and phylogenic analysis were performed to confirm the subtypes of Blastocystis sp.. In the end, determine the relationship between the parasite subtypes and the host by contrasting and analyzing.Results and Conclusion: A total of 720 fecal samples from 132 animal species, 140 (19.44%) were PCR positive for Blastocystis sp., with 43.67% (31/71), 5.89% (7/119), 34.87% (53/152), 2.56% (1/39), 16.67% (2/12), 22.22% (4/18), 37.50% (3/8) and 12.96% (39/301) in Non-human primates (NHP), Carnivora, Artiodactyla, Perissodactyla, Proboscidea, Diprotodontia, Rodentia and aves, respectively. Sequence and phylogenetic analysis showed ten known subtypes (ST1, ST2, ST3, ST4, ST5, ST7, ST8, ST10, ST14, and ST23) of Blastocystis sp. in the present study, with ST10 as the predominant subtype in all animals. However, there were differences in dominant subtypes among the eight groups of animals, and ST2 of NHP, ST3 of Carnivora, ST10 of Artiodactyla, ST23 of Proboscidea, ST1 of Diprotodontia, ST1 and ST4 of Rodentia and ST5 of aves, respectively. This is the first report of Blastocystis sp. infection in 48 species to the best of our knowledge. These results not only enriched the host range of Blastocystis sp. and filled the data of vacant animals but also provided a reference for preventing and controlling Blastocystis sp. infection between animals and humans.
The Distribution of COVID 19 Variants by Daily Cases in California, United States
Authors: Zargar Roshanak 
Published: March 2022

Abstract
Background. The new Covid-19 variants' emergence and rapid global spread pose a daunting public health emergency. The Covid-19 virus continues mutating by the emergence of new variants with high transmissibility. This paper illustrates the timing and lifespan of COVID-19 variants in California from January 2021 to February 2022 to provide a window showing the time frames of active variants.Methods: This study used publicly available data on COVID-19, including covid variants of daily cases from the California Department of Health. Data from January 1, 2021, to February 28, 2022, were retrieved.Results: The findings showed that the distribution of the variants could be divided into two main phases: January 2021 to June 2021 and from mid-summer 2021 to February 2022 from December to March. The first phase has more variations, while Omicron and Delta dominate the second phase.Conclusions: This research indicates that variants have different characteristics and life spans. Recent variants have mutated faster than other older precedents. The lag between variants' international initiation for the first variants was longer than Omicron and Delta.
Outcome of Gynecologic Cancer Patients With The Covid-19 Infection : A Systematic Review And Meta Analysis
Authors: Winata I Gde Sastra , Simatupang Januar , Polim Arie A , Togar Yakob , Tondang Advenny Elisabeth 
Published: March 2022

Abstract
Objective
Cancer is comorbidity, which can lead to progressive worsening of Covid-19 with increased mortality. This is a systematic review and meta-analysis to get evidence of adverse outcomes of Covid-19 in gynecologic cancer.
Methods
Searches through PubMed, Google Scholar, ScienceDirect, and medRxiv to find articles on the outcome of gynecologic cancer with Covid-19 (24 July 2021-19 February 2022). Newcastle-Ottawa Scale tool used to evaluate the quality of included studies. Pooled odds ratio (OR), 95% confidence interval (CI), random-effects model were presented. This study was registered to PROSPERO (CRD42021256557).
Results
We accepted 49 studies with (1994 gynecologic cancer with Covid-19). Covid-19 infection was lower in gynecologic cancer vs hematologic cancer (OR 0.71, CI 0.56–0.89, p 0.003). Severe Covid and death were lower in gynecologic cancer vs lung and hematologic cancer (OR 0.36, CI 0.16–0.80, p 0.01), (OR 0.26, CI 0.10–0.67 p 0.005), (OR 0.52, CI 0.43–0.63, p < 0.0001), (OR 0.65, CI 0.49–0.87, p 0.003) respectively. Increased Covid death is seen in gynecologic cancer vs breast, non-covid cancer, and non-cancer covid (OR 1.51, CI 1.20–1.90, p 0.0004), (OR 12.21, CI 8.39–17.77, p < 0.0001), (OR 3.06, CI 2.32–4.04, p < 0.0001) respectively.
Conclusion
Gynecologic cancer had increased Covid-19 adverse outcomes compared to non-cancer, breast cancer, non-metastatic, and Covid-19 negative population. Gynecologic cancer had lowered Covid-19 adverse outcomes compared to other cancer types, lung cancer, and hematologic cancer. Lack of age and comorbidities stratification due to limited data were limitations. These findings may aid health policies and services during the ongoing global pandemic.