bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of suicide decedents with no federally funded mental health service contact in the 12 months before death in a population‐based sample of Australians 45 years of age and over
Authors: Carter Gregory , Sperandei Sandro , Spittal Matthew J. , Chitty Kate , Clapperton Angela , Page Andrew 
Published: November 2022

AbstractIntroductionMore than half of suicide decedents have no contact with mental health services 12 months before death. It is uncertain if they have different characteristics than decedents who use mental health services.MethodsA case‐series design. Participants 45 years and older, who died by suicide (2006–2018). Comparisons were made between those who did and did not have contact with mental health services, using individually linked data from federal services in the Medicare Benefits Schedule (MBS) and Pharmaceutical Benefits Scheme (PBS).ResultsOf 186 cases, 71% had no contact with mental health services. Physical health services were used equally by 75%. Psychiatric medication use was uncommon, except for antidepressants, 50% with mental health service contact and 20% with no contact. Older age, lower income, involuntarily unemployed, firearms as suicide method, greater physical disability, less functional impairment due to emotional problems and lesser proportions with mental illness, were associated with no contact with mental health services.ConclusionsFor suicide prevention, middle‐older aged adults may have less requirement for mental health intervention, and greater requirement for the development of complementary interventions focused on physical health and social issues, which are not necessarily best delivered by clinical mental health services.
Theory of mind, gender, gains in friendships versus peer acceptance and anxious solitude from middle childhood through early adolescence
Authors: Gazelle Heidi , Lundin Jessie K. Shafer , Bosacki Sandra L. 
Published: November 2022

AbstractThis investigation proposes that theory of mind (ToM) may be related more strongly to change in friendships than peer acceptance in late middle childhood through early adolescence, and examines the relation between ToM and anxious solitude. Fourth grade ToM was tested as a predictor of change in reciprocated friendships, peer acceptance, and anxious solitude from 4th to 7th grade, and, conversely, reciprocated friendships, peer acceptance, and anxious solitude in 3rd grade were tested as predictors of 4th grade ToM. Gender moderation of these relations was evaluated. Participants were 688 American public‐school children (51.5% girls), 193 of whom completed a ToM questionnaire in 4th grade. In 3rd–7th grade children and their peers reported reciprocated friendship, and peers reported peer acceptance and anxious solitude annually. A multi‐group (gender split) autoregressive cross‐lagged panel analysis modeled relations between ToM and reciprocated friendship, peer acceptance, and anxious solitude over time. Consistent with hypotheses, girls’ more advanced 4th grade ToM predicted incremental gains in their number of friendships two years later, but not their peer acceptance. In contrast, boys’ more advanced 4th grade ToM did not predict change in their number of friendships or peer acceptance over time. Gender differences in the relation between ToM and friendship are discussed in the context of gender‐specific peer relations patterns in late middle childhood and early adolescence. Additionally, more advanced 4th grade ToM predicted gains in anxious solitude in middle school for both genders. This somewhat surprising result is discussed in relation to ToM assessment and peer relations in anxious solitary children.
Pan‐genome and multi‐parental framework for high‐resolution trait dissection in melon (Cucumis melo)
Authors: Oren Elad , Dafna Asaf , Tzuri Galil , Halperin Ilan , Isaacson Tal , Elkabetz Meital , Meir Ayala , Saar Uzi , Ohali Shachar , La Thuy , Romay Cinta , Tadmor Yaakov , Schaffer Arthur A. , Buckler Edward S. , Cohen Roni , Burger Joseph , Gur Amit 
Published: November 2022

SUMMARYLinking genotype with phenotype is a fundamental goal in biology and requires robust data for both. Recent advances in plant‐genome sequencing have expedited comparisons among multiple‐related individuals. The abundance of structural genomic within‐species variation that has been discovered indicates that a single reference genome cannot represent the complete sequence diversity of a species, leading to the expansion of the pan‐genome concept. For high‐resolution forward genetics, this unprecedented access to genomic variation should be paralleled and integrated with phenotypic characterization of genetic diversity. We developed a multi‐parental framework for trait dissection in melon (Cucumis melo), leveraging a novel pan‐genome constructed for this highly variable cucurbit crop. A core subset of 25 diverse founders (MelonCore25), consisting of 24 accessions from the two widely cultivated subspecies of C. melo, encompassing 12 horticultural groups, and 1 feral accession was sequenced using a combination of short‐ and long‐read technologies, and their genomes were assembled de novo. The construction of this melon pan‐genome exposed substantial variation in genome size and structure, including detection of ~300 000 structural variants and ~9 million SNPs. A half‐diallel derived set of 300 F2 populations, representing all possible MelonCore25 parental combinations, was constructed as a framework for trait dissection through integration with the pan‐genome. We demonstrate the potential of this unified framework for genetic analysis of various melon traits, including rind color intensity and pattern, fruit sugar content, and resistance to fungal diseases. We anticipate that utilization of this integrated resource will enhance genetic dissection of important traits and accelerate melon breeding.
Multiplexed cellular profiling identifies an organoselenium compound as an inhibitor of CRM1‐mediated nuclear export
Authors: Jimenez Lucia , Mayoral‐Varo Victor , Amenábar Carlos , Ortega Judit , Sequeira João G. N. , Machuqueiro Miguel , Mourato Cristiana , Silvestri Romano , Angeli Andrea , Carta Fabrizio , Supuran Claudiu T. , Megías Diego , Ferreira Bibiana I. , Link Wolfgang 
Published: November 2022

AbstractChromosomal region maintenance 1 (CRM1 also known as Xpo1 and exportin‐1) is the receptor for the nuclear export controlling the intracellular localization and function of many cellular and viral proteins that play a crucial role in viral infections and cancer. The inhibition of CRM1 has emerged as a promising therapeutic approach to interfere with the lifecycle of many viruses, for the treatment of cancer, and to overcome therapy resistance. Recently, selinexor has been approved as the first CRM1 inhibitor for the treatment of multiple myeloma, providing proof of concept for this therapeutic option with a new mode of action. However, selinexor is associated with dose‐limiting toxicity and hence, the discovery of alternative small molecule leads that could be developed as less toxic anticancer and antiviral therapeutics will have a significant impact in the clinic. Here, we report a CRM1 inhibitor discovery platform. The development of this platform includes reporter cell lines that monitor CRM1 activity by using red fluorescent protein or green fluorescent protein‐labeled HIV‐1 Rev protein with a strong heterologous nuclear export signal. Simultaneously, the intracellular localization of other proteins, to be interrogated for their capacity to undergo CRM1‐mediated export, can be followed by co‐culturing stable cell lines expressing fluorescent fusion proteins. We used this platform to interrogate the mode of nuclear export of several proteins, including PDK1, p110α, STAT5A, FOXO1, 3, 4 and TRIB2, and to screen a compound collection. We show that while p110α partially relies on CRM1‐dependent nuclear export, TRIB2 is exported from the nucleus in a CRM1‐independent manner. Compound screening revealed the striking activity of an organoselenium compound on the CRM1 nuclear export receptor.
Modeling Fluid Inertia Effects in External Gear Machines Through Lumped Parameter Approach
Authors: Mistry Zubin , Vacca Andrea , Dhar Sujan , Rigosi Manuel 
Published: November 2022

Abstract
Lumped parameter modeling is a consolidated technique for analyzing the fluid dynamic behavior of positive displacement machines, owing to their computational swiftness and the ease to integrate other physical domains affecting the operation of the machine. With some very limited exceptions, this modeling technique typically neglects fluid inertia and momentum effects. This paper proposes an approach to study the effects of fluid inertia affecting the pressurization and depressurization of the tooth space volumes of an external gear pump. The approach is based on considering the fluid inertia in the pressurization grooves and, inside the control volumes with a peculiar sub-division. Further, frequency dependent friction is also modeled to provide realistic damping of the fluid inside these channels. Validation of the model has been performed by comparing the lumped parameter model with a full three-dimensional Navier Stokes solver. The quantities compared such as tooth space volume pressures and outlet volumetric flowrate show a good match between the two approaches for varying operating speeds. The paper lastly, also discusses what operating conditions and geometries play a significant role that governs the necessity to model such fluid inertia effects in the first place.
A Cylinder Enabling Algorithm for Reduction in Low Frequency Pulsation From Digital Displacement Pumps
Authors: Dumnov Daniil , Caldwell Niall 
Published: November 2022

Abstract
Digital Displacement® hydraulic pumps are a type of radial-piston machine with solenoid operated on/off valves used to individually control the pressurization of each cylinder on a stroke-by-stroke basis, thus adjusting the pump’s overall displacement. Previously developed cylinder enabling strategies based on using full strokes can lead to low frequency vibration, whereas partial stroke strategies pose challenges in audible noise and component lifetime. A new enabling algorithm is proposed, Quantized Part Stroke (QPS), which seeks to minimize the low frequency content in the pump output, as well as limiting noise due to actuating the valve near mid-stroke. The operating displacement fraction is quantized such that only integer fractions of displacement are permitted, the fraction’s denominator relating directly to a minimum allowable frequency in the pump output. This quantization is applied such that the chosen displacement fraction is higher than the demand and then part strokes are used to exactly achieve the desired flow. Simulation results are presented comparing this algorithm with well-known alternatives, as well as test data from a 12-cylinder pump showing a clear decrease in low frequency pulsation in the hydraulic system pressure, without a significant change in audible noise from the pump.
Practical Evaluation of a Control Concept for a Remote Controlled 1.8T Excavator Using a 3D Input Device
Authors: Haas Christian , Schneider Arne , Opgenoorth Andreas , Schmitz Katharina 
Published: November 2022

Abstract
Tele-remote control of excavators poses challenges for operators. While conventional operation already requires complex motor skills and thus extensive training, both the feeling for the machine and the 3D view are missing in a tele-remote scenario. This can result in operators being unable to estimate distances and performing movements more slowly and less accurately. Possible solutions to facilitate the operation of remote-controlled construction machinery are to improve visibility conditions through more sophisticated camera systems, or to provide motion feedback to the operator station. However, these solutions may be limited, for example, by higher demands on data transmission. Another solution could be to adapt the control of the machine. Therefore, this paper presents a control concept for a tele-remote operated excavator using a 3D-input device. First the test machine and the general structure of the control concept are introduced. For controlling the tool-position, an approach with direct position control and one with secondary velocity control are presented. For evaluation, both approaches are tested and compared. Finally, operator trials are presented and the usability of the concept is evaluated.
State-of-the-art of Variable Displacement Technologies for Radial Piston Hydraulic Machines
Authors: Darnet Justin , Bideaux Éric 
Published: November 2022

Abstract
The hydraulic motor is one of the main parts of hydrostatic transmission implemented in some off-road vehicles for instance. It uses hydraulic power to convey energy from the generator of the machine to the wheels and other actuators. As the hydraulic circuit may contain several hydraulic actuators, each of them needs to be controlled independently since the only control of the pump displacement may impact the entire circuit and all its actuators simultaneously.
To properly drive the vehicle, the hydraulic actuators have to be accurately controlled. This paper aims to present a state-of-the-art of the different ways of changing hydraulic pumps/motors displacement ratios because it is one of the most important parameter to control the torque/speed (resp. pressure/flow rate). Some hydraulic machines enable a continuous change of this displacement ratio thanks to their specific architectures, whereas other machines use hydraulic valves to pilot this ratio. Most of the technologies, from variable displacement axial solutions to new digitally modulated displacement machines will be presented and compared. Digital Displacement solutions and their valve timing control solutions will then be depicted. Finally, technological locks in Digital Displacement will be exposed based on the modeling of a radial piston motor.
Ear magnetic discs to prevent cauliflower ear: a case gone wrong
Authors: Ang Wan Wei , Foley Gary , Laycock Juliet , McKay-Davies Iain 
Published: November 2022

We present a case of pinna pressure necrosis secondary to the use of ear magnetic discs, used in the management of auricular haematoma. A man in his 20s sustained a left auricular haematoma while wrestling. His coach aspirated the haematoma and advised the use of commercially available compression magnets on either side of the pinna to prevent haematoma reaccumulation. 5 days later, he presented to accident and emergency with left ear pain and swelling. Perichondritis was evident on examination and the compression magnets were removed to reveal significant pressure necrosis of the pinna. The skin underlying the placement of magnets (both anteriorly and posteriorly) was black and necrosed, with erosion of the underlying cartilage. In addition to this, the haematoma had reaccumulated in the surrounding pinna. The haematoma was drained via an incision, and pressure dressing applied with dental rolls. The patient was given a course of oral antibiotics to manage the perichondritis. He was reviewed regularly in the ear, nose and throat emergency clinic to monitor wound healing. 3 months later, he was reviewed in the otology clinic; there was persistent helical rim deformity, and delayed cartilage augmentation was advised.This case highlights the importance of prompt and effective management of auricular haematoma, to prevent long-term deformity. Commercially available pinna magnets for auricular haematoma should be used with caution, and patients should be counselled as such.
Homozygous truncating variant inMAN2A2causes a novel congenital disorder of glycosylation with neurological involvement
Authors: Mahajan Sonal , Ng Bobby George , AlAbdi Lama , Earnest Paul Daniel James , Sosicka Paulina , Patel Nisha , Helaby Rana , Abdulwahab Firdous , He Miao , Alkuraya Fowzan S , Freeze Hudson H 
Published: November 2022

BackgroundEnzymes of the Golgi implicated in N-glycan processing are critical for brain development, and defects in many are defined as congenital disorders of glycosylation (CDG). Involvement of the Golgi mannosidase, MAN2A2 has not been identified previously as causing glycosylation defects.MethodsExome sequencing of affected individuals was performed with Sanger sequencing of theMAN2A2transcript to confirm the variant. N-glycans were analysed in patient-derived lymphoblasts to determine the functional effects of the variant. A cell-based complementation assay was designed to assess the pathogenicity of identified variants using MAN2A1/MAN2A2 double knock out HEK293 cell lines.ResultsWe identified a multiplex consanguineous family with a homozygous truncating variant p.Val1101Ter in MAN2A2. Lymphoblasts from two affected brothers carrying the same truncating variant showed decreases in complex N-glycans and accumulation of hybrid N-glycans. On testing of this variant in the developed complementation assay, we see the complete lack of complex N-glycans.ConclusionOur findings show that pathogenic variants in MAN2A2 cause a novel autosomal recessive CDG with neurological involvement and facial dysmorphism. Here, we also present the development of a cell-based complementation assay to assess the pathogenicity of MAN2A2 variants, which can also be extended to MAN2A1 variants for future diagnosis.
Rate advancement measurement for lung cancer and pleural mesothelioma in asbestos-exposed workers
Authors: Azzolina Danila , Consonni Dario , Ferrante Daniela , Mirabelli Dario , Silvestri Stefano , Luberto Ferdinando , Angelini Alessia , Cuccaro Francesco , Nannavecchia Anna Maria , Oddone Enrico , Vicentini Massimo , Barone-Adesi Francesco , Cena Tiziana , Mangone Lucia , Roncaglia Francesca , Sala Orietta , Menegozzo Simona , Pirastu Roberta , Tunesi Sara , Chellini Elisabetta , Miligi Lucia , Perticaroli Patrizia , Pettinari Aldo , Bressan Vittoria , Merler Enzo , Girardi Paolo , Bisceglia Lucia , Marinaccio Alessandro , Massari Stefania , Magnani Corrado 
Published: November 2022

IntroductionExposure to asbestos increases the risk of lung cancer and mesothelioma. Few studies quantified the premature occurrence of these diseases in asbestos-exposed workers. Focus on premature disease onset (rate advancement or acceleration) can be useful in risk communication and for the evaluation of exposure impact. We estimated rate advancement for total mortality, lung cancer and pleural mesothelioma deaths, by classes of cumulative asbestos exposure in a pooled cohort of asbestos cement (AC) workers in Italy.MethodThe cohort study included 12 578 workers from 21 cohorts, with 6626 deaths in total, 858 deaths from lung cancer and 394 from pleural malignant neoplasm (MN). Rate advancement was estimated by fitting a competitive mortality Weibull model to the hazard of death over time since first exposure (TSFE).ResultAcceleration time (AT) was estimated at different TSFE values. The highest level of cumulative exposure compared with the lowest, for pleural MN AT was 16.9 (95% CI 14.9 to 19.2) and 33.8 (95% CI 29.8 to 38.4) years at TSFE of 20 and 40 years, respectively. For lung cancer, it was 13.3 (95% CI 12.0 to 14.7) and 26.6 (95% CI 23.9 to 29.4) years, respectively. As for total mortality, AT was 3.35 (95% CI 2.98 to 3.71) years at 20 years TSFE, and 6.70 (95% CI 5.95 to 7.41) at 40 years TSFE.ConclusionThe current study observed marked rate advancement after asbestos exposure for lung cancer and pleural mesothelioma, as well as for total mortality.
Ordovician of the Bohemian Massif
Authors: Kraft Petr , Linnemann Ulf , Mergl Michal , Bruthansová Jana , Laibl Lukáš , Geyer Gerd 
Published: November 2022

Abstract
The lower Paleozoic succession of central Europe exposed in the Bohemian Massif is a classic area of geology with a long-standing tradition of research dating back to the eighteenth century. The Ordovician rocks form parts of sections in several units that sit on the Cadomian basement. These sedimentary and volcano-sedimentary fills of partial depressions in the basement are relics of the system of rift basins in the Gondwanan margin reflecting the rifting of the Rheic Ocean. The Ordovician sections are related to the subsidence period during the extensional regime accompanied by volcanism. They are underlain by Neoproterozoic or Cambrian rocks and continue up usually without breaks. After closure of the Rheic Ocean owing to the Gondwana–Laurussia collision, the Ordovician successions were incorporated into the Variscan Orogen belt and preserved in denudation relics such as the Bohemian Massif and its units. Ordovician strata with Gondwanan shelf affinities can be traced along the Variscans from Spain to central Europe, and are reflected in the regional stratigraphic scale based mainly on the succession in the Prague Basin. The Ordovician fill of this accumulation centre, together with relics of another preserved in the Schwarzburg Anticline, represents the main exposures in the Bohemian Massif. The individual features of the Ordovician successions, such as facies developments, fossil associations and volcanism, make them model areas both for understanding the palaeogeographic and geotectonic evolution of the peri-Gondwanan margin and a stratigraphic standard for a cool-water regime.
Cargo-Specific Role for Retriever Subunit VPS26C in Hepatocyte Lipoprotein Receptor Recycling to Control Postprandial Triglyceride-Rich Lipoproteins
Authors: Vos Dyonne Y. , Wijers Melinde , Smit Marieke , Huijkman Nicolette , Kloosterhuis Niels J. , Wolters Justina C. , Tissink Joël J. , Pronk Amanda C.M. , Kooijman Sander , Rensen Patrick C.N. , Kuivenhoven Jan Albert , van de Sluis Bart 
Published: November 2022

Background:
The copper metabolism MURR1 domains/coiled-coil domain containing 22/coiled-coil domain containing 93 (CCC) complex is required for the transport of low-density lipoprotein receptor (LDLR) and LRP1 (LDLR-related protein 1) from endosomes to the cell surface of hepatocytes. Impaired functioning of hepatocytic CCC causes hypercholesterolemia in mice, dogs, and humans. Retriever, a protein complex consisting of subunits VPS26C, VPS35L, and VPS29, is associated with CCC, but its role in endosomal lipoprotein receptor transport is unclear. We here investigated the contribution of retriever to hepatocytic lipoprotein receptor recycling and plasma lipids regulation.

Methods:
Using somatic CRISPR/Cas9 gene editing, we generated liver-specific VPS35L or VPS26C-deficient mice. We determined total and surface levels of LDLR and LRP1 and plasma lipids. In addition, we studied the protein levels and composition of CCC and retriever.

Results:
Hepatocyte VPS35L deficiency reduced VPS26C levels but had minimal impact on CCC composition. VPS35L deletion decreased hepatocytic surface expression of LDLR and LRP1, accompanied by a 21% increase in plasma cholesterol levels. Hepatic VPS26C ablation affected neither levels of VPS35L and CCC subunits, nor plasma lipid concentrations. However, VPS26C deficiency increased hepatic LDLR protein levels by 2-fold, probably compensating for reduced LRP1 functioning, as we showed in VPS26C-deficient hepatoma cells. Upon PCSK9 (proprotein convertase subtilisin/kexin type 9)-mediated LDLR elimination, VPS26C ablation delayed postprandial triglyceride clearance and increased plasma triglyceride levels by 26%.

Conclusions:
Our study suggests that VPS35L is shared between retriever and CCC to facilitate LDLR and LRP1 transport from endosomes to the cell surface. Conversely, retriever subunit VPS26C selectively transports LRP1, but not LDLR, and thereby may control hepatic uptake of postprandial triglyceride-rich lipoprotein remnants.