bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of an Algorithm for Cervical High-Grade Squamous Intraepithelial Lesion Based on Breath Print Analysis
Authors: Dokter Laura A. , à Nijeholt Jeanine H. , Rigterink Bernadette M. , de Lange Natascha M. , de Haan Harm H. , van Eijndhoven Hugo W.F. , Joostens Mieke , Kruse Arnold-Jan 
Published: October 2022

Objectives
This study was designed to develop an algorithm for the diagnosis of cervical high-grade squamous intraepithelial lesions (HSIL), based on patterns of volatile organic compounds, evaluated using an e-nose.

Methods
For this pilot study, the study population consisted of a group of 25 patients with histologically confirmed HSIL and a group of 26 controls. Controls consisted of women visiting the outpatient department for gynecological complaints unrelated to cancer. Women had a negative high-risk human papillomavirus and/or normal cytology (negative for intraepithelial lesions of malignancy) of their most recent test performed in the context of participation in routine cervical cancer screening. Breath tests were performed and labeled with the correct diagnosis. Machine-learning techniques were used to develop a model for predicting HSIL. Based on the receiver operating characteristics curve, both sensitivity and specificity were calculated.

Results
Individual classifications of all patients with HSIL and controls, as calculated by the model, showed a sensitivity of 0.88 (95% CI = 0.68–0.97) and specificity of 0.92 (95% CI = 0.73–0.99). The positive predictive value and the negative predictive value were 0.92 (95% CI = 0.72–0.99) and 0.89 (95% CI = 0.70–0.97), respectively. The Cohen κ coefficient was 0.80.

Conclusions
E-nose can detect distinctive patterns of volatile organic compounds between cervical HSIL patients and controls. Validation of the algorithm in further studies is necessary before possible implementation into daily practice.
Synchronous invasive ductal breast cancer with clear cell renal carcinoma: a rare case report with review of literature
Authors: Bapir Rawa , Hammood Zuhair D. , Omar Sami S. , Salih Abdulwahid M. , Kakamad Fahmi H. , Najar Kayhan A. , Salih Karzan M. 
Published: October 2022

Introduction:
Multiple cancer refers to the emergence of 2 or more cancers in a single patient. This study aims to report a rare case of clear cell renal cancer associated with invasive breast ductal carcinoma.

Case presentation:
A 56-year-old female presented with a left breast mass. Clinical examination revealed a mobile left side breast mass at the left upper quadrant. An irregular hypoechoic mass was seen on ultrasound, and mammography showed a unifocal spiculated mass seated on the pectoralis muscle. Computed tomography of the chest and abdomen revealed a cystic mass lesion in the cortex of the lower pole of the left kidney. Partial nephrectomy and wide local breast mass excision with sentinel lymph node biopsy were done. Histopathologic results confirmed associated invasive ductal breast carcinoma with clear renal cell carcinoma.

Discussion:
In the treatment of renal cell carcinoma with multiple tumors, several factors should be interpreted, including the tumor stage, evaluation for the presence of metastasis, and whether the kidney tumor is accessible for operation.

Conclusion:
The term multiple cancer refers to the development of more than one tumor in a single patient that could be synchronous or metachronous. The association of invasive ductal breast cancer with clear renal cell carcinoma is a sparse entity.
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Authors: Bhaskar M Maanasa , Rajamanikam Soundarya , Raj Sruthi , Sistla Sujatha , Nichanahalli Kubera Siddappa 
Published: October 2022

Introduction : Melioidosis is an emerging life threatening infectious disease caused by Gram negative bacilli Burkholderia pseudomallei found in contaminated soil and surface ground water . It often presents with varied clinical manifestations and has a high mortality if left untreated due to lack of clinical suspicion. Here we report a rare and fatal case of puerperal sepsis due to Burkholderia pseudomallei in a young woman.
Case report: A 25-year-old female comes to a medical consultation at day 43 post partum with complains of fever on and off for the past 40 days associated with vomiting. On per speculum examination, greenish discharge from the cervix was seen and a right adnexal mass was felt which was cystic in consistency, non-tender and pushing cervix to the left side. Exploratory laparotomy was carried out and pus was drained from below the rectus sheath, and from right tubo-ovarian mass. Peripheral blood sample and the pus samples collected intraoperatively grew Burkholderia pseudomallei . The patient expired on the 5 th post operative day due to septic shock with disseminated intravascular coagulation secondary to puerperal sepsis.
Conclusion: Melioidosis is a fatal but treatable disease when it is promptly diagnosed. In a country like India, where tuberculosis is highly endemic, underdiagnosis of melioidosis can be a common consequence. Clinicians and Microbiologists should have high index of suspicion especially in individuals with underlying illness.
A Multi-Center, Controlled Human Infection Study of Influenza A (H1N1)pdm09 in Healthy Adults
Authors: Ortiz Justin R. , Bernstein David I. , Hoft Daniel F. , Woods Christopher W. , McClain Micah T. , Frey Sharon E. , Brady Rebecca C. , Bryant Christopher , Wegel Ashley , Frenck Robert W , Walter Emmanuel B. , Abate Getahun , Williams Sarah R. , Atmar Robert L. , Keitel Wendy A. , Rouphael Nadine , Memoli Mathew J. , Makhene Mamodikoe K. , Roberts Paul C. , Neuzil Kathleen M. 
Published: October 2022

ABSTRACTBackgroundInfluenza controlled human infection model (CHIM) studies can advance development of vaccines and therapeutics. Our objective was to evaluate the associations between baseline challenge virus-specific hemagglutination inhibition (HAI) and microneutralization (MN) titers and subsequent symptomatic influenza virus infection.MethodsWe enrolled healthy adults aged 18 through 49 years in a multisite CHIM study using influenza A/Bethesda/MM2/H1N1, an A/California/04/2009/H1N1pdm-like virus (NCT04044352). We excluded persons vaccinated against influenza within the previous six months. We collected serial safety labs, serum for HAI and MN, and nasopharyngeal swabs for RT-PCR testing. Analyses used the putative seroprotective titer of ≥40 for HAI and MN. The primary clinical outcome was mild-to-moderate influenza disease (MMID), defined as ≥1 post-challenge positive qualitative RT-PCR test with a qualifying symptom/clinical finding.FindingsOf 76 participants given influenza virus challenge, 54 (71.1%) experienced MMID. Clinical illness post-challenge was generally very mild. MMID attack rates among participants with baseline titers ≥40 by HAI and MN were 64.9% and 67.9%, respectively, while MMID attack rates among participants with baseline titers <40 by HAI and MN were 76.9% and 78.3%, respectively. The estimated odds of developing MMID decreased by 19% (odds ratio=0.81; 95% CI: 0.62, 1.06; p=0.126) for every two-fold increase in baseline HAI. There were no deaths, serious adverse events, or other significant adverse events.InterpretationIn a multi-site influenza CHIM study, we assured the safety of our participants and achieved a 71.1% attack rate of MMID. High baseline HAI and MN were associated with protection from illness.
Evaluating the Evidence Quality Supporting the Role of Acupuncture for Herpes Zoster Pain: An Umbrella Review of Systematic Reviews and Meta-analyses
Authors: Li Jianhong , Liu Kun , Ruan Wenjia , Lin Guohua 
Published: October 2022

ObjectiveAcupuncture is one of the most effective means to treat herpes zoster, especially during the acute stage. It can shorten the duration of pain, with a low incidence of residual neuralgia. The aim of this review was to assess the methodological and evidence quality of systematic reviews and meta-analyses on acupuncture for herpes zoster pain and provide clinical suggestions.MethodsEight databases were searched from their inception to Feb 2022. Methodological quality was evaluated by using the Assessment of Multiple Systematic Reviews 2, and the Grading of Recommendations Assessment Development and Evaluation was applied to evaluate the quality of the evidence.ResultsNineteen SRs/MAs were included according to the inclusion criteria. Assessment of Multiple Systematic Reviews 2 results showed that the methodological quality was low for 16 studies and very low for 3 studies. According to the Grading of Recommendations Assessment Development and Evaluation, 43 outcome indicators were evaluated in this review, and the results showed that the evidence quality was high in three, medium in twelve, low in twenty-three, and very low in five. Compared with drugs for herpes zoster, acupuncture can reduce pain and the incidence of postherpetic neuralgia. Acupuncture at Jia ji acupoint was generally one of the most effective methods.ConclusionsOur study showed that fire needle, acupuncture at Jia ji, pricking collaterals and bloodletting are beneficial therapies for the clinical practice of herpes zoster. However, the methodological and evidence quality of the included studies were low. More rigorous design and comprehensive investigations by systematic reviews and meta-analyses are needed.Strengths and limitations of this studyThis study is a comprehensive review to assess the methodological and evidence quality of recent SRs/MAs on acupuncture for herpes zoster pain, we mainly focused on pain symptoms, for example VAS score, time for pain relief, incidence of PHN, with qualitative analysis of efficiency. Only systematic reviews published in Chinese and English were included, so the conclusions of this study may not be generalizable. Some gray documents may be omitted. Subjective factors could not be completely eliminated during screening and evaluation.PROSPERO registration number CRD42022304247.
The Long-term Mediation Role of Cytokines on the Causal Pathway from Maternal Gestational Age to Offspring Visual System: Lifecourse-Network Mendelian Randomization
Authors: Hou Lei , Li Yunxia , Kang Lili , Li Xiaoying , Li Hongkai , Xue Fuzhong 
Published: October 2022

AbstractBackgroundGestational duration has a non-negligible impact on eye diseases. However, the long-term role of cytokines on the causal relationship of maternal gestational age on offspring visual impairment remains unclear.MethodsWe perform a lifecourse-network Mendelian randomization (MR) to explore the causal relationships among maternal gestational duration (from EGG and iPSYCH, N=84,689), neonatal/adult cytokines (from the NHGRI-EBI Catalog, N=764/4,618) and adult eye diseases (from FinnGen consotium, N=309,154) using summary-level data from large genome-wide association studies. Multiplicative random effects inverse variance weighted (IVW) and multivariable-IVW method are the main analysis methods and the other 15 pleiotropy-robust methods, weak IV-robust methods and outliers-robust methods are performed as auxiliary methods.ResultsWe find that maternal gestational age (early preterm birth, preterm birth, gestational duration and postterm birth) has causal relationships with 42 eye diseases. Specially, four neonatal cytokines: TNF-α, IL10, GROA and CTACK, as well as four adult cytokines: CTACK, IL10, IL12p70 and IL6.26 are mediators in the causal relationships between early preterm birth and preterm birth to 8 eye diseases. However, after adjusting for these mediators, null direct causal effect of early preterm birth and preterm birth on 8 eye diseases can be found. In addition, there is no mediator in the causal relationships from gestational duration and postterm birth to eye diseases.ConclusionThe influences of maternal gestational duration on the offspring eye diseases through cytokines are long-term and lifecourse.
Genetic, demographic and clinical variables act synergistically to impact neurodevelopmental outcomes in children with single ventricle heart disease
Authors: Miller Thomas A. , Hernandez Edgar J. , Gaynor J. William , Russell Mark W. , Newburger Jane W. , Chung Wendy , Goldmuntz Elizabeth , Cnota James F. , Zyblewski Sinai C. , Mahle William T. , Zak Victor , Ravishankar Chitra , Kaltman Jonathan R. , McCrindle Brian W. , Clarke Shanelle , Votava-Smith Jodie K. , Graham Eric M. , Seed Mike , Rudd Nancy , Bernstein Daniel , Lee Teresa M. , Yandell Mark , Tristani-Firouzi Martin 
Published: October 2022

ABSTRACTRecent large-scale sequencing efforts have shed light on the genetic contribution to the etiology of congenital heart defects (CHD); however, the relative impact of genetics on clinical outcomes remains less understood. Outcomes analyses using genetic data are complicated by the intrinsic severity of the CHD lesion and by interactions with conditionally dependent clinical variables. Here we apply Bayesian Networks, an explainable Artificial Intelligence solution, to describe the intertwined relationships between clinical variables, demography, and genetics in a cohort of children with single ventricle CHD. As isolated variables, a damaging genetic variant in a gene related to abnormal heart morphology and prolonged ventilator support following stage I palliative surgery increased the probability of having a low Mental Developmental Index (MDI) score at 14 months of age by 1.9- and 5.8-fold, respectively. However, in combination, these variables acted synergistically to further increase the probability of a low MDI score by 10-fold. Likewise, genetic information was predictive of a favorable neurodevelopmental outcome. For example, the absence of a damaging variant in a known syndromic CHD gene and a shorter post-operative ventilator support increased the probability of a normal MDI score 1.7- and 2.4-fold, respectively, but in combination increased the probability of a good outcome by 59-fold. Our analyses suggest a modest genetic contribution to neurodevelopmental and growth outcomes as isolated variables, similar to known clinical predictors. By contrast, genetic, demographic, and clinical variables interact synergistically to markedly impact clinical outcomes. These findings underscore the importance of capturing and quantifying the impact of damaging genomic variants in the context of multiple, conditionally dependent variables, such as pre- and post-operative factors, and demography.
Gene expression profiling and protein-protein network analysis revealed prognostic hub biomarkers linking cancer risk in type 2 diabetic patients
Authors: Kasera Harshita , Shekhawat Rajveer Singh , Yadav Pankaj , Singh Priyanka 
Published: October 2022

AbstractType 2 diabetes mellitus (T2DM) and cancer are highly prevalent diseases imposing major health burden globally. Several epidemiological studies indicate increased susceptibility to cancer in T2DM patients. However, genetic factors linking T2DM with cancer are poorly studied so far. We used computational approach on the raw gene expression data of peripheral blood mononuclear cells of Homo sapiens available at the gene expression omnibus (GEO) database, to identify shared differentially expressed genes (DEGs) in T2DM and three common cancer types namely, pancreatic (PC), liver (LC) and breast cancer (BC). Additional functional and pathway enrichment analysis of identified common DEGs highlighted involvement of important biological pathways including cell cycle events, immune system process, cell morphogenesis, gene expression and metabolism. Furthermore, we retrieved the PPI network for crucial DEGs obtained from above analysis to deduce molecular level interactions. Based on the result of network analysis, we found 8, 5 and 9 common hub genes in T2DM vs PC, T2DM vs LC and T2DM vs BC, respectively. Overall, our analysis identified important genetic markers potentially able to predict the chances of pancreatic, liver and breast cancer onset in T2DM patients.
Evaluating the potential of marine invertebrate and insect protein hydrolysates to reduce fetal bovine serum in cell culture media for cultivated fish production
Authors: Batish Inayat , Zarei Mohammad , Nitin Nitin , Ovissipour Reza 
Published: October 2022

AbstractThe use of fetal bovine serum (FBS) and the price of the cell culture media are the key constraints for developing serum-free cost-effective media. This study aims to replace or reduce the typical 10% serum application in fish cell culture media by applying protein hydrolysates from insects and marine invertebrate species for the growth of Zebrafish embryonic stem cells (ESC) as the model organism. Protein hydrolysates were produced from Black soldier fly (BSF), cricket, oyster, mussel, and lugworm with high protein content, suitable functional properties, adequate amino acids composition, and the degree of hydrolysis from 18.24 to 33.52%. Protein hydrolysates at low concentrations from 0.001 to 0.1 mg/mL in combination with 1 and 2.5% serum significantly increased cell growth compared to the control groups (5 and 10% serum) (P < 0.05). All protein hydrolysates with concentrations of 1 and 10 mg/mL were found to be toxic to cells and significantly reduced cell growth and performance (P < 0.05). However, except for cricket, all hydrolysates were able to restore or significantly increase cell growth and viability with 50% less serum at a concentration of 0.001, 0.01, and 0.1 mg/mL. Although cell growth was enhanced at lower concentrations of protein hydrolysates, cell morphology was altered due to the lack of serum. Lactate dehydrogenase (LDH) activity results indicated that BSF and lugworm hydrolysates did not alter the cell membrane. In addition, light and fluorescence imaging revealed that cell morphological features were comparable to the 10% serum control group. Overall, lugworm and BSF hydrolysates reduced serum by up to 90% while preserving excellent cell health.
Topological incongruence between Median-Joining Networks and Bayesian inference phylogenies
Authors: Kong Sungsik , Sánchez-Pacheco Santiago J. , Murphy Robert W. 
Published: October 2022

1AbstractInferring phylogenies among intraspecific individuals often yields unresolved relationships (i.e., polytomies). Consequently, methods that compute distance-based abstract networks, like Median-Joining Networks (MJNs), are thought to be more appropriate tools for reconstructing such relationships than traditional trees. MJNs visualize all routes of relationships in the form of cycles, if needed, when traditional approaches cannot resolve them. However, the MJN method is a distance-based phenetic approach that does not involve character transformations and makes no reference to ancestor–descendant relationships. Although philosophical and theoretical arguments challenging the implication that MJNs reflect phylogenetic signal in the traditional sense have been presented elsewhere, an empirical comparison with a character-based approach is needed given the increasing popularity of MJN analysis in evolutionary biology. Here, we use the conservative Approximately Unbiased (AU) test to compare 85 cases of branching patterns of cycle-free MJNs and Bayesian Inference (BI) phylogenies using datasets from 55 empirical studies. By rooting the MJN analyses to provide directionality, analyses find substantial disagreement between computed MJNs and posterior distributions on BI phylogenies. The branching patterns in MJNs and BI phylogenies show significantly different relationships in 37.6% of cases. Among the relationships that were not significantly different, 96.2% show different sets of relationships. Our results indicate that the two methods provide different measures of relatedness in a phylogenetic sense. Finally, our analyses also support previous observations of the statistical hypothesis testing by reconfirming the over-conservativeness of the Shimodaira–Hasegawa test versus the AU test.
Tunable image-mapping optical coherence tomography
Authors: Lee Jaeyul , Du Xiaoxi , Park Jongchan , Cui Qi , Iyer Rishyashring R. , Boppart Stephen A. , Gao Liang 
Published: October 2022

AbstractWe present tunable image-mapping optical coherence tomography (TIM-OCT), which can provide optimized imaging performance for a given application by using a programmable phase-only spatial light modulator in a low-coherence full-field spectral-domain interferometer. The resultant system can provide either a high lateral resolution or a high axial resolution in a snapshot without moving parts. Alternatively, the system can achieve a high resolution along all dimensions through a multiple-shot acquisition. We evaluated TIM-OCT in imaging both standard targets and biological samples. Additionally, we demonstrated the integration of TIM-OCT with computational adaptive optics in correcting sample-induced optical aberrations.
Assessing a multivariate model of brain-mediated genetic influences on disordered eating in the ABCD cohort
Authors: Westwater Margaret L. , Mallard Travis T. , Warrier Varun , Bethlehem Richard A.I. , Scheinost Dustin , Grillon Christian , Fletcher Paul C. , Seidlitz Jakob , Ernst Monique 
Published: October 2022

AbstractEating disorders (EDs) are complex psychiatric conditions that often emerge during adolescence, and affected individuals frequently demonstrate high rates of psychiatric comorbidity, particularly with depressive and anxiety disorders. Although risk for EDs reflects both genetic and neurobiological factors, knowledge of how genetic risk for EDs relates to neurobiology and psychiatric symptoms during critical developmental periods remains limited. We therefore implemented a novel multivariate framework, which sought to advance knowledge of the etiology of EDs by simultaneously estimating associations between genetic risk, brain structure and ED-related psychopathology symptoms in over 4,500 adolescents of European ancestry from the Adolescent Brain and Cognitive Development study (M(SD)age=119.29(7.49) months). Polygenic scores for anorexia nervosa (AN PGS) and body mass index (BMI PGS) were generated and related to three morphometric brain features— cortical thickness, surface area and subcortical grey matter volume—and to latent psychopathology factors using structural equation modeling. We identified a three-factor structure of ED-related psychopathology symptoms: eating, distress and fear factors. Increased BMI PGS were uniquely associated with greater eating factor scores, whereas AN PGS were unrelated to psychopathology factors. Moreover, genetic risk for high BMI and for AN had distinct neural correlates, where greater BMI PGS predicted widespread increases in cortical thickness and reductions in surface area while AN PGS were nominally related to reduced caudate volume. Altered default mode and visual network thickness was associated with greater eating factor scores, whereas distress and fear factor scores reflected a shared reduction in somatomotor network thickness. Our novel findings indicate that greater genetic risk for high BMI and altered cortical thickness of canonical brain networks underpin ED symptomatology in early adolescence. As neurobiological factors appear to shape disordered eating earlier in the life course than previously thought, these results underscore the need for early detection and intervention efforts for EDs.