bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive profiling and characterization of cellular microRNAs in response to Coxsackievirus A10 infection in bronchial epithelial cells
Authors: Hu Yajie , Wang Lan , Zhong Mingmei , Zhao Wei , Wang Yujue , Song Jie , Zhang Yunhui 
Published: March 2022

Abstract
Coxsackievirus A10 (CV-A10), the causative agent of hand, foot, and mouth disease (HFMD), caused a series of outbreaks in recent years and often leads to neurological impairment, but a clear understanding of the disease pathogenesis and host response remains elusive. Cellular microRNAs (miRNAs), a large family of non-coding RNA molecules, have been reported to be key regulators in viral pathogenesis and virus-host interactions. However, the role of host cellular miRNAs defensing against CV-A10 infection is still obscure. To address this issue, we systematically analyzed miRNA expression profiles in CV-A10-infected 16HBE cells by high-throughput sequencing methods in this study. It allowed us to successfully identify 312 and 278 miRNAs with differential expression at 12 h and 24 h post-CV-A10 infection, respectively. Among these, 4 miRNAs and their target genes were analyzed by RT-qPCR, which confirmed the sequencing data. Gene target prediction and enrichment analysis revealed that the predicted targets of these miRNAs were significantly enriched in numerous cellular processes, especially in regulation of basic physical process, host immune response and neurological impairment. And the integrated network was built to further indicate the regulatory roles of miRNAs in host-CV-A10 interactions. Consequently, our findings could provide a beneficial basis for further studies on the regulatory roles of miRNAs relevant to the host immune responses and neuropathogenesis caused by CV-A10 infection.
Contraceptive Preferences and Adoption Following Female Genital Fistula Surgery in Uganda: A Mixed- Methods Study
Authors: Ayadi Alison M. El , Nalubwama Hadija , Painter Caitlyn , Kakaire Othman , Miller Suellen , Barageine Justus , Byamugisha Josaphat , Obore Susan , Korn Abner , Harper Cynthia C. 
Published: March 2022

Abstract
Background: Female genital fistula, largely caused by prolonged obstructed labour, is treated by surgical repair. Avoiding pregnancy for a minimum period post-repair is generally recommended to ensure adequate healing, so contraceptive preferences and use are important for optimizing post-repair outcomes. Methods: We examined contraceptive preferences and use in the year following genital fistula surgery using mixed-methods. 60 Ugandan women were enrolled starting in December 2014 with data collection completed in August 2016. Sociodemographic characteristics, fistula-related incontinence, sexual activity, contraceptive use and pregnancy status were recorded quarterly for 12 months after surgery. In-depth interviews among purposively selected participants (n=30) explored intimate relationships, sexual experiences, reproductive intentions, and contraceptive use. Results: Median participant age was 28 years (interquartile range [IQR]: 21–36 years), and almost half (48%) were married or living with partners. Counselling was provided to 97% of participants on delaying sexual intercourse and 59% received counselling on contraception. Sexual activity was reported by 7% after 3 months and 50% after 12 months. 6 (10%) women became pregnant during the study period. Eighty-three percent reported not trying for pregnancy. Among sexually active participants, contraceptive use was low at baseline (36%) but increased (to 75%) at 12 months. Interview participants who desired children immediately were young, had fewer children, experienced stillbirth at fistula development, and felt pressure from partners. Women adopted contraception to fully recover from fistula surgery and avoid adverse outcomes. Others simply preferred to delay childbearing. Reasons cited for not adopting contraception included fecundity perceptions, family planning misconceptions, and healthcare provider advice.Conclusions: Contraceptive use following genital fistula repair increased over time, but unmet need still existed, and misconceptions were prevalent despite some counselling. Provision of comprehensive patient-centred contraceptive counselling at the time of fistula surgery and beyond is important for helping women to optimize healing from fistula and minimize recurrence, as well as to meet their own reproductive preferences.
The acceleration degradation processes of different aged refuses with the forced aeration for landfill reclamation
Authors: Lou Ziyang , Liu Yihang , Huang Qiujie , Cheng Zhaowen , Cao Weihua , Wang Xianghui , Yang Changfu , Liu Hui , Song Jia , Wang Luochun 
Published: March 2022

Abstract
Forced aeration is one of the promising ways to accelerate landfill reclamation, and understanding the relation between aeration rates and waste properties is the prerequisite to implementing forced aeration under the target of energy saving and carbon reduction. In this work, landfill reclamation processes with forced aeration were simulated using aged refuses (ARs) of 1, 4, 7, 10, and 13 disposal years, and the potential of field application was also investigated based on a field project, to identify the degradation rate of organic components, the O2 consumption efficiency and their correlations to microbes. It was found that the removal rate of organic matters declined from 20.3% (AR1) to 12.6% (AR13), and that biodegradable matter (BDM) decreased from 5.2–2.4% at the set aeration rate of 0.12 L O2/kg DM/day, respectively. A linear relationship between degradation rate constant (K) of BDM and disposal ages (x) was established: K=-0.0002193x + 0.0091 (R2 = 0.854), suggesting that BDM might be a suitable indicator to reflect the stabilization of ARs. The cellulose/ lignin ratio decrease rate for AR1 (18.3%) was much higher than AR13 (3.1%), while the corresponding humic-acid/ fulvic-acid ratio increased from 1.44 to 2.16. The dominant bacteria shift from Corynebacterium (9.2%), Acinetobacter (6.6%), and Fermentimonas (6.5%), genes related to the decompose of biodegradable organics, to Stenotrophomonas (10.2%) and Clostridiales (3.7%), which were associated with the humification. The aeration efficiencies of lab-scale tests were at the range of 5.4–11.8 g BDM/ L O2 for ARs with disposal ages of 1–13 years, and in situ landfill reclamation, ARs with disposal age of 10–18 years were around 1.9–8.8 g BDM/ L O2, as the disposal age decreased. The increased discrepancy was observed in ARs of lab-scale and field scale, indicating that the forced aeration rate should be adjusted based on aged refuses and the unit compartment combined, to reduce the operation cost.
3D finite element simulation for tool temperature distribution and chip formation during drilling of Ti6Al4V alloy
Authors: Zhu Zhaoju , Yunqi Zhu , Xinhui Sun , Chuhang Gao , Zhimao Lin , Bingwei He 
Published: March 2022

Abstract
A comprehensive 3D finite element simulation study has been conducted to investigate the tool temperature distribution along cutting edge and chip formation during drilling of Ti6Al4V alloy by using commercial finite element software Abaqus/Explicit. The Johnson-Cook material constitutive model and material failure criterion were implemented across the formulations to achieve the tool temperature and chip formation in the drilling process. The effect of different mesh sizes on simulation results was also analyzed. To assess and verify the accuracy of the simulation model, the corresponding experiment studies were carried out by measuring the thrust force, tool temperature, and chip morphology. Compared results show that the predicted thrust force, temperature distribution, and chip morphology are in good agreement with those tested from experiments. According to the combination of both simulations and experiments, it can be not only found the whole varying pattern of the thrust force but also reveal that the temperature decreases from drill center to outer corner along the primary cutting edge. In addition, it can provide a more detailed and profound knowledge about chip formation. All these are assumed to be recommendations for the optimization of tool geometry and drilling processing.
In-memory mathematical operations with spin-orbit torque devices
Authors: You Long 
Published: March 2022

Abstract
Analogue arithmetic operations are the most fundamental mathematical operations used in image and signal processing as well as artificial intelligence (AI). In-memory computing offers high performance and energy-efficient computing paradigm. To date, in-memory analogue arithmetic operation with emerging nonvolatile devices were usually implemented using discrete components, which limits the scalability and blocks large scale integration. Here, we experimentally demonstrate a prototypical implementation of in-memory analogue arithmetic operations (summation, subtraction and multiplication), based on in-memory electrical current sensing units using spin-orbit torque (SOT) devices. The proposed analogue arithmetic operation structures are smaller than the state-of-the-art CMOS counterparts by several orders of magnitude. Moreover, data to be processed and computing results can be locally stored, or the analogue computing can be done in the nonvolatile SOT devices, which were exploited to experimentally implement image edge detection and signal amplitude modulation with simple structure. Furthermore, we constructed an artificial neural network (ANN) with SOT devices based synapses to realize pattern recognition with high accuracy of ~95%.
Responsive nucleus accumbens deep brain stimulation restores eating control in severe obesity
Authors: Halpern Casey , Shivacharan Rajat , Rolle Cammie , Barbosa Daniel , Cunningham Tricia , Feng Austin , Johnson Noriah , Safer Debra , Bohen Cara , Keller Corey , Buch Vivek , Parker Jonathan , Azagury Dan , Tass Peter , Bhati Mahendra , Malenka Robert , Lock James 
Published: March 2022

Abstract
Cravings that precede loss of control (LOC) over food consumption present an opportunity for intervention in patients suffering from binge eating disorder (BED). Here, we used responsive deep brain stimulation (DBS) to record NAc electrophysiology during food cravings preceding LOC eating in two patients with BED and severe obesity (NCT03868670). Increased NAc low-frequency oscillations prominent during food cravings were used to guide DBS delivery. Over 6 months, we observed improved self-control of food intake and weight loss. These findings provide early support for restoring inhibitory control with electrophysiologically-guided NAc DBS. Further work is required to determine scalability of this approach. Trial Registration # NCT03868670.
Analysis Of Influencing Factors Of Plasma Concentration Of Oxcarbazepine Monotherapy In Children With Epilepsy
Authors: Yao Nannan , Huang Shan , Huang Aiwen , Song Hongtao 
Published: March 2022

Abstract
Purpose: The aim of this study was to investigate the factors affecting the plasma concentration of oxcarbazepine (OXC) monotherapy in children with epilepsy. Methods: We recruited 125 children with epilepsy who received OXC monotherapy. 27 single nucleotide polymorphisms were detected by MassARRAY genotyping technology to evaluate the influence of related factors on the plasma concentration of OXC monotherapy. The plasma concentration of 10-hydroxycarbamazepine (MHD), the main active metabolite of OXC, was determined by high performance liquid chromatography (HPLC). Results: The weight was found to be associated with concentration-dose ratio and maintenance dose. The duration of seizure was found to be associated with concentration-dose ratio. Carriers of the UGT1A4 rs2011425 mutant allele A or G had higher MHD plasma concentration than wild homozygous TT type. Carriers of the UGT2B15 rs1902023 mutant allele C had higher plasma concentration than wild homozygous AA types. The concentration-dose ratio of patients with ABCB1 rs1045642 mutation homozygous GG type is higher than heterozygous AG type. The concentration-dose ratio of patients with CACNA1H rs2753325 heterozygous AG type was higher than mutant homozygous GG type. We established a concentration prediction equation for OXC monotherapy in children with epilepsy. Conclusion: This study clarified the association of weight, duration of seizure, and gene polymorphisms of UGT1A4 rs2011425, UGT2B15 rs1902023, ABCB1 rs1045642 and CACNA1H rs2753325 with MHD plasma concentration. The concentration prediction equation of OXC monotherapy in children with epilepsy was constructed, which provides a reference for individualized clinical administration.
Pb-doped CuO thin films synthetized by sol-gel method
Authors: touka nassim , TABLI D. , TOUKA N. , BADARI K. , SELMI N. 
Published: March 2022

Abstract
Sol-gel thin films of undoped CuO and Pb-doped CuO were deposited using the spin coating method. In three thin layers, the lead was introduced at two different concentrations (5 and 7%). The films produced were in the tenorite phase, with high purity and crystallinity, according to XRD structural characterization. The grain size of CuO films is affected by the Pb concentration and decreases as the Pb concentration increases, although the strain and dislocation density values ​​increase as the Pb doping ratio increases. The structure of CuO was confirmed in all films by Raman and FTIR studies. In the presence of Pb dopants, SEM examinations revealed cubic and spherical nanostructured surfaces. With the value of the optical band gap increasing from 1.92 to 2.74 eV, there was a significant association between increasing Pb doping concentration and widening of the optical band gap.
Protection of insect neurons by erythropoietin/CRLF3-mediated regulation of pro-apoptotic acetylcholinesterase
Authors: Knorr Debbra Y. , Schneider Kristin , Büschgens Luca , Förster Jan , Georges Nadine S. , Geurten Bart R.H. , Heinrich Ralf 
Published: March 2022

Abstract
Cytokine receptor-like factor 3 (CRLF3) is a highly conserved but largely uncharacterized orphan cytokine receptor that shares structural similarity with vertebrate classical erythropoietin receptor. CRLF3-mediated neuroprotection in insects can be stimulated with human erythropoietin and involves partly similar anti-apoptotic mechanisms as erythropoietin-mediated neuroprotection in mammals. To identify potential mechanisms of CRLF3-mediated neuroprotection we studied the expression and function of acetylcholinesterase which promotes apoptosis in different cell types, including mammalian neurons. We exposed primary brain neurons from Locusta migratoria and Tribolium castaneum to apoptogenic stimuli and/or dsRNA to interfere with acetylcholinesterase gene expression and compared survival and/or acetylcholinesterase expression in the presence or absence of the CRLF3 ligand erythropoietin. Hypoxia increases both apoptotic cell death and expression of both acetylcholinesterase-coding genes ace-1 and ace-2. Both ace genes give rise to single transcripts in both normal and apoptogenic condictions. Pharmacological inhibition of both acetylcholinesterases and RNAi-mediated knockdown of either ace-1 or ace-2 expression prevent hypoxia-induced apoptosis of primary brain neurons. Activation of CRLF3 with protective concentrations of rhEpo prevents the increased expression of pro-apoptotic acetylcholinesterase with larger impact on ace-1 than on ace-2. In contrast, high concentrations of rhEpo that commonly (and seemingly paradoxically) cause death of insect and mammalian neurons induced ace-1 expression and hence promoted apoptosis in insect neurons. Our study confirms the cell-intrinsic role of acetylcholinesterase as a major regulator of apoptotic death, that was previously described in mammalian neurons only. Moreover, we identify a mechanism (prevention of upregulation of pro-apoptotic acetylcholinesterase), by which CRLF3 activation mediates neuroprotection under apoptogenic conditions. Since both apoptosis and CRLF3 are conserved throughout the animal kingdom, the direct link between cytokine/CRLF3 activation and suppression of increased acetylcholinesterase expression underlying neuroprotection in insects may also be present in other cell types and other non-insect species.
Conducting Community-Capacity-Based Evaluation Framework of Importance and Performance for Earthquake Disaster Management
Authors: Lin Bih-Chuan , Lee Chun-Hung 
Published: March 2022

Abstract
The frequency and intensity of natural disasters have been increasing in recent decades, especially earthquakes are one of the causes of major natural disasters. Improving community adaptive against disasters based on community capacity has gradually become an effective means of coping with disaster risks and improve residents' well-being. However, few studies have explored the correlation between community adaptive to disasters and awareness of disaster preparedness. In the study, we establish an evaluation adaptive framework of development community earthquake disaster management (CEDM) based on community-capacity and their perspectives under importance-performance analysis (IPA) method in the eastern Taiwan, and identifies the factors. Features that affect the differentiation of community residents' participation on CEDM program are divided into four dimensions: 1) risk; 2) learn; 3) cope; and 4) change, revealing the degree of disaster prevention ability and awareness of urban and suburban residents, as well as residents' satisfaction with the government's community disaster prevention measures. The higher the residents' awareness of disaster prevention, the stronger the ability to adapt to disasters; the higher the ability of the government and non-profit organizations to cooperate with the community to deal with disasters, the stronger the community's ability to manage disasters. The research contributes a novel conceptual framework and the findings point to directions for the sustainable development of CEDM project management in Taiwan.
The Development And Psychometric Evaluation Of COVID-19 Staff Questionnaire For Infectious Disease Outbreak Readiness And Preparedness (SQIDORP)
Authors: Jokwiro Yangama , Urbanavicius Tracy , Robinson Ainsley M , Scott Cathy , Islam Md Rafiqul 
Published: March 2022

Abstract
Background: The COVID-19 pandemic has inundated the capacity of hospitals across the globe, exhausting resources, and placing extreme burden on health care workers (HCWs). Hospital preparedness during infectious disease outbreak involves development and implementation of appropriate strategies, procedures, and adequate training for HCWs. Reliable and valid tools to evaluate the perception of HCWs on the effectiveness of hospital preparedness strategies are imperative and literature is yet to fill that gap. Methods: Items for ‘The Staff Questionnaire for Infectious Disease Outbreak Readiness and Preparedness (SQIDORP)’ were selected from literature that addressed hospital preparedness during novel pandemic outbreaks. The SQIDORP was distributed within a regional hospital in Victoria, Australia. Psychometric evaluation included estimates of reliability and factor analysis while factors associated with the questionnaire were explored using regression analysis. Results: Omega coefficient of 0.89, Cronbach’s alpha coefficient of 0.88 and item-total correlations (>0.3) indicated adequate reliability of the SQIDORP. Factor Analysis yielded three meaningful latent factors that are effectiveness of training (Factor 1), self-confidence (Factor 2) and risk to self and stress (Factor 3). Demographic factors did not influence the correlation with SQIDORP. However, rating ‘the current plan for management of COVID-19 in your ward’ and ‘personal knowledge/skills in caring for patients with COVID-19’ had significant positive correlation and accounted for 33% of the variance in readiness and preparedness using SQIDORP (R2=0.33, F=10.227, P<0.001). Conclusion: Most of the items of SQIDORP questionnaire achieved adequate internal consistence reliability. This is a valuable tool that can be utilized by hospitals to explore aspects of preparedness and give insights to the knowledge, skills, and mental health of HCWs, as perceived by the HCW themselves.
Repellent Activity Of Carvacrol And Timol Encapsulated With Yeast Cell Wall Against Amblyomma Sculptum And Rhipicephalus Sanguineus (Sensu Lato) Nymphs
Authors: Costa Jhone Robson Silva , Vale Tassia Lopes , Silva Geovane Ferreira , Cavalho Naylene Silva Sales , Lima Aldilene Silva , Costa-Junior Lívio Martins , Luz Hermes Ribeiro 
Published: March 2022

Abstract
The main way to avoid contact with ticks and consequently tick-borne disease is the use of synthetic repellents. The search of new repellent compounds to increase the possibilities of use in strategies controls are necessary. The present study evaluated the repellent activity of two natural terpenes carvacrol and thymol in each one two different formulation (encapsulated and nonencapsulated with yeast cell wall) against the ticks Amblyomma sculptum and Rhipicephalus sanguineus sensu lato nymphs. Nymphs of A. sculptum and R. sanguineus s.l. of a single generation were used. The vertical filter paper repellency assay were performed with different concentration of both terpenes encapsulated and nonencapsulated in yeast cell wall. The repellent concentration 50% (CR50) were calculated to each compound formulation. Both carvacrol and thymol (encapsulated and nonencapsulated), had a repellent activity against A. sculptum and R. sanguineus s.l nymphs. Amblyomma sculptum was more sensitive to nonencapsulated carvacrol (RC50 values: 0.0032 to 0.0082 mg/cm2 after 1 and 15 min) (P < 0.05), while R. sanguineus s.l. was more sensitive to encapsulated carvacrol (RC50 values: 0.00008 to 0.0035 mg/cm2 after 1 and 15 min) (P < 0.05). Among tick species, R. sanguineus s.l. was more sensitive for most compounds than A. sculptum (P < 0.05). Although with distinct repellent activities, carvacrol and thymol encapsulated can be a promising alternative to synthetic repellents against A. sculptum and R. sanguineus s.l.