bluuebunny/crossref_metadata_2025_split huggingface dataset:
P-513 Quality of life assessment of women undergoing in vitro fertilization treatment in Kazakhstan
Authors: Suleimenova M , Lokshin V , Karibayeva S , Glushkova N , Terzic M 
Published: June 2022

Abstract

Study question
What is the quality of life of Kazakhstani women with infertility and what additional factors can affect it?

Summary answer
The FertiQoL questionnaire showed the low quality of life of Kazakhstani women and a significant affect some factors.

What is known already
Studies showed that infertility problems are among the saddest events in people's lives. It is reported that women undergoing IVF procedures are experiencing various types of psychological burdens like stress, anxiety and depression.

Study design, size, duration
This cross-sectional study involved women of reproductive age undergoing an IVF cycle at a large IVF center in Kazakhstan in the period from September 1, 2020 to September 31, 2021.

Participants/materials, setting, methods
The survey was carried out using the Google Form program and was sent to patients of large IVF clinic of Kazakhstan by the attending physician for an online survey. Out of 500 women who were given questionnaires, 453 women completely filled out. Questionnaire results data were entered into SPSS version 26.0 for aggregation and statistical analysis. Two-sample Student's t-test or one-way analysis of variance (ANOVA) was used to test for differences between groups.

Main results and the role of chance
Total FertiQoL was 59.6±11.3. 292 women with primary infertility and 161 with secondary infertility. According to the main indicators as Total FertiQoL and Core FertiQoL, the results of the scales were significantly higher (p &lt; 0.001). The source of income for infertility treatment affects the level of Treatment FertiQoL. Cronbach's α coefficients of the FertiQoL subscale averaged 0.8. Cronbach's α coefficients of the FertiQoL subscale averaged 0.8.
We examined the satisfaction scores by FertiQoL, dividing them into two groups according to the presence of children (Table 3). FertiQoL scores were statistically significantly higher in the group with children than in the group with primary infertility in all areas (p &lt; 0.05), except for Relation, Tolerability (p = 0.65, p = 0.59).

Limitations, reasons for caution
This study cross-sectional and was conducted in the countries of the Commonwealth of Independent States (CIS) for the first time. We want to continue research in cooperation with other CIS countries.

Wider implications of the findings
The duration of infertility, age and low economic status negatively affect the quality of life of women. The availability of subsidies from the state for infertility treatment improves the quality of life. The health expenditure percentage of GDP may affect the overall level of satisfaction of women.

Trial registration number
IRB-A108
P-530 tRNA fragments in follicle fluid can be explored in Diminished Ovarian Reserve patients as a marker of IVF outcomes
Authors: Dawkins J , Donelson F , Bossa D , Zhang C , Sathe A , Owen D , Nam Y 
Published: June 2022

Abstract

Study question
Can tRNA fragments in follicle fluid be explored for markers that predict blastocyst formation in patients with diminished ovarian reserve?

Summary answer
tRNA-Ser-CGA-4 is up-regulated in the follicle fluid of patients with diminished ovarian reserve (DOR)

What is known already
tRNA fragments are a novel class of small non-coding RNAs that have been shown to play regulatory roles in reproductive biology.

Study design, size, duration
Non-Randomized case control study in patients presenting for IVF treatment at a University affiliated fertility clinic from September 2020- January 2021. Thirty three patients were included: 15 patients with DOR and 18 controls.

Participants/materials, setting, methods
Patients were stimulated by appropriate protocols according to their age, diagnosis and AMH.
At retrieval, non-bloody follicle fluid (FF) was retrieved for after removing the cumulus-oophorus complex. FF was centrifuged at 1600 g x 10 mins and filtered with 80 nm filter for analysis.

Main results and the role of chance
There were no differences between the two groups with respect to age, BMI and duration of infertility. The mean age was 35.1 years
As anticipated, the mean AMH, antral follicle count and number of eggs retrieved were statistically lower in the DOR groups compared with controls (&lt;0.05)
The fertilization rate was 61.2% in DOR vs 79.5% in controls
The blastocyst rate was 39.5% in DOR patients and 50.6% in controls
Total RNA yield was lower in DOR patients, as 50% of the DOR samples had undetectable levels (p &lt; 0.001).
Samples were predominantly processed in duplicate. Input into the pipeline was paired end data which showed an overall tRNA fragment count that was higher in the DOR population. Log2FC showed an up-regulation of the tRNA fragments tRNA-Ser-CGA-4, tRNA-Cys-GCA-15, tRNA-Cys-GCA-10 and tRNA-Cys-GCA-19 in the DOR population.

Limitations, reasons for caution
Undetectable levels in follicle fluid of some of the DOR patients introduced selection bias
Larger sample size and broader applicability across various ethnic groups.

Wider implications of the findings
Follicle fluid can be explored to give insight into the signaling pathways of ovarian biology
Non-invasive method of assessing oocyte competence
Useful in explaining the pathophysiology of DOR
Can these findings assist is in the identification of therapeutic targets to delay the progression of age related/non-age related DOR?

Trial registration number
not applicable
P-539 Effects of gender of chromosomal translocation's carrier on pre-implantation genetic testing for structural rearrangement (PGT-SR) outcomes
Authors: Houissa Ediss H , Reignier A , Lammers J , Moradkhani K , Freour T , Loubersac S 
Published: June 2022

Abstract

Study question
Do the outcomes of PGT-SR cycles vary according to the gender of chromosomal translocation’s carrier?

Summary answer
The rate of balanced embryos was significantly higher when translocation carrier was a male as compared to female, but clinical outcomes were not significantly different.

What is known already
It has been well described that chromosomal translocations lead to very high rate of unbalanced embryos, and have a subsequent negative impact on blastulation, implantation, miscarriage and live birth rates.PGT-SR allows selecting balanced embryos and increases the chances of pregnancy, while decreasing the risk of micarriage in couples with chromosomal translocations. However, whether the gender of translocation carrier is associated with significantly different impact on PGT-SR outcomes is not known.

Study design, size, duration
This is a monocentric retrospective cross-sectional study conducted in all PGT-SR cycles performed between 2015 and 2020.
A total of 603 cycles, carried out in 283 couples, were included in the analysis.

Participants/materials, setting, methods
Two groups have been formed according to the gender of translocation carrier, Group 1 for female carriers (107 couples, 229 cycles), and group 2 for male (175 couples, 373 cycles).
The rate of balanced embryos, ovarian stimulation parameters, number of oocytes, biopsy rate, clinical pregnancy rate and live birth rate were compared between both groups.

Main results and the role of chance
Female age and infertility etiologies were comparable between both groups.
Mean total dose of gonadotrophin and peak E2 levels were not significantly different in both groups (2422± 895 units vs 2290 ± 858 respectively, p &gt; 0.05) (2460± 1346 pg/ml vs 2497 ± 1397, p &gt; 0.05). There was no significant difference in the number of oocytes and biopsied embryos rate between Group 1 and Group 2.
The rate of balanced embryo after PGT-SR was significantly lower in group 1 (female carriers) than in group 2 (21.1% vs 24.8% respectively, p = 0.036). Finally, biochemical pregnancy rate and live birth rate per transfer were not statistically different between both groups (46.2% vs 43.2% and 20.3% vs 18.5% respectively, p &gt; 0.05).

Limitations, reasons for caution
The retrospective nature of the study was its main limitation. PGT-A was not used, preventing from concluding on embryo euploidy rate.

Wider implications of the findings
Further fundamental studies should be performed to explore the respective mechanisms involved in chromosomal rearrangements according to gender. Our study suggests that a higher number of oocytes could be targeted in female translocation carriers in order to increase the probability of obtaining at least one balanced embryo.

Trial registration number
2
P-562 Extended carrier screening using the qCarrier Plus® test in the field of reproductive medicine: retrospective results after five years of experience
Authors: Forne's Garcia N , Garcia Cruz R , Carreño Gago L , Borgia A , Segura Puimedon M , Armengol Dulcet L 
Published: June 2022

Abstract

Study question
To determine the reproductive risk of women and couples for the tested recessive and X-linked diseases.

Summary answer
An increased reproductive risk has been identified in 6% of women and in 6% of couples due to the presence or coincidence of pathogenic variants.

What is known already
Carrier testing is an established method to determine the reproductive risk of couples undergoing reproduction techniques. This risk assessment may differ depending on the analyzed diseases and the studied population.

Study design, size, duration
Observational retrospective study in more than 10,000 samples from gamete donors and patients undergoing assisted reproduction treatment, referred to our laboratory during 5 years for carrier screening using the qCarrier Plus® test.

Participants/materials, setting, methods
This test uses massive sequencing technology to screen for mutations causing 328 autosomal recessive and X-linked diseases. For the present study, only pathogenic or probably pathogenic variants were considered and variants of uncertain significance (VUS) were excluded.

Main results and the role of chance
Our data indicate that around 80% of the individuals in our cohort are carriers of at least one pathogenic variant, while 50% are carriers for multiple diseases. An increased reproductive risk has been identified in approximately 6% of women tested for being carriers of pathogenic variants in the X chromosome genes, and in 6% of couples due to coincidence of pathogenic variants causing the same disease. Furthemore, 40 of the genes included in our panel have a carrier frequency greater than 1%.

Limitations, reasons for caution
There are both technical and human limitations that might interfere in the analytical process, and that might hamper the identification of some genetic variants.

Wider implications of the findings
Not applicable.

Trial registration number
Not applicable
P-573 Women's age is the best success predictor in a cohort of poor responders: A retrospective analysis according to POSEIDON criteria
Authors: Amaral M.E , Monteleone P.A.A , Ejzenberg D , Baracat E.C 
Published: June 2022

Abstract

Study question
Are there differences between patients classified according to the Poseidon criteria regarding IVF sucess rates?

Summary answer
POSEIDON groups differ from each other. Woman's age, more than the ovarian reserve, is the main factor of poor prognosis in IVF cycles.

What is known already
Poor ovarian response to stimulation in an IVF cycle has an estimated incidence of 9-24%. The criteria used to characterize this group of patients are heterogeneous, making it difficult to address risk factors, to determine prognosis and to propose novel treatment options. The most used diagnostic criteria are the Bologna criteria and more recently the Poseidon classification.

Study design, size, duration
An observational, retrospective cohort study, based on 558 ART cycles performed between 2015 and 2018 at a single center in Brazil.

Participants/materials, setting, methods
Group division was acording to POSEIDON criteria: groups 1A (n = 15), 1B (n = 19), 2A(n = 48), 2B (n = 59), 3 (n = 17) and 4 (n = 50). Two control groups were stablished for comparison purposes: C1 with no previous poor response, normal ovarian reserve and women's age &lt; 35 years (n = 157) and C2 with women's age ≥ 35 years (n = 193). Data was obteined from clinical records and analyzed with SPSS program.

Main results and the role of chance
Groups 3, 4 and 2A of Poseidon had less oocytes recovered (p &lt; 0,001). Group 4 formed fewer embryos (p = 0,001). A previous history of poor response in younger patients wasnt't found to translate in a poor response in a second cycle, since there was no difference between groups 1A and 1B compared to group C1 regarding number of oocytes retrieved. Groups 1B and C1 had a higher clinical pregnancy rate (p = 0,028) and groups 2 and 4 had lower pregnancy rates per started cycle (p &lt; 0,001).

Limitations, reasons for caution
Retrospective and observational study.

Wider implications of the findings
The findings of this study play a role in improving patients counselling. The results are encouraging for young women with normal reserve and a previous bad response, since they weren't found to have poor outcomes in a new cycle. Woman's age should be the main criteria to determine patient prognosis.

Trial registration number
not applicable
P-574 Ovarian Revitalisation using Platelet Rich Plasma
Authors: Singh P , Rastogi R 
Published: June 2022

Abstract

Study question
Can we rejuvinate ovaries (with low ovarian reserve) by injecting fresh platelet rich plasma ?

Summary answer
With ovarian PRP injections, Ovarian reserve will increase and number of follicles increases in treated ovaries .

What is known already
Till date no definitive treatment is available to restore function of ovaries. Few drugs e.g. granulocyte colony stimulating factor etc tried for the same but no satisfactory result was achieved. Oral ovulogens and gonadotropins cannot produce satisfactory number of ova in these patients. So they were forced to go for donar ova. By using ovarian PRP injections couple can have a chance of their own biological child.

Study design, size, duration
we conducted the pilot study involving injection of autologous fresh platelet rich plasma injection into the ovaries of subfertile females by comparison of their fertility parameters viz. hormonal profile involving measurements of blood levels of follicular stimulating hormone (FSH), luteinizing hormone (LH), anti-Mullerian hormone (AMH), prostaglandin E2 levels (PGE2); antral follicular count (AFC); number of mature follicles (NMF) and number of retrieved oocytes (NRO) in both pre-PRP and post-PRP states.

Participants/materials, setting, methods
Forty-five subfertile females of more than 20 years were included in our study . All the patients underwent fresh autologous PRP injection under transvaginal ultrasonographic guidance. Besides the demographic data, each patient’s hormonal profile (including blood levels of AMH, FSH, LH, PGE2), AFC, NMF and NRO in both pre-PRP and post-PRP state were recorded in a predesigned proforma. Statistical evaluation of the recorded data was done using appropriate methods and tools.

Main results and the role of chance
Mean age, weight, height, body mass index and years of subfertility of the patients in our study was 31.27yrs, 58.38kg, 154.84cm, 24.37kg/m2 and 8.44years respectively. The mean blood levels of AMH increased significantly in post-PRP state (1.6 vs 2.4pg/ml) in contrast to significant decrease in blood level of FSH (21.4 vs 12.5mIU/ml), LH (15.1 vs 5.9mIU/ml) & PGE2 (162.7 vs 66pg/ml). The AFC, NMF & NRO also increased in post-PRP state relative to pre-PRP state. The differences in above levels and numbers were statistically significant.

Limitations, reasons for caution
Autologous PRP injection into ovaries improves hormonal profile, AFC and number of mature follicles as well as retrieved oocytes. Furthur followup is needed to know weather our patients concieve spontaneously or they will need some kind of assisted tecniques to concieve a pregnency.

Wider implications of the findings
Autologous PRP injection into ovaries improves hormonal profile, AFC and number of mature follicles as well as retrieved oocytes, it can be utilized as a revitalisation tool to boost fertility. Our study results reinforce the above concept. However, outcome-based studies are needed to further validate the results of our study.

Trial registration number
TMU/IEC/19-20/0113
P-605 Biomechanical characteristics of the ovarian cortex in POI patients and functional outcomes after drug-free IVA
Authors: Méndez M , Ferreri J , Calafell J.M , Otero J , Farré R , Manau D , Carmona F , Fàbregues F 
Published: June 2022

Abstract

Study question
Is there a relationship between the biomechanical characteristics of ovarian cortex of POI patients and the resumption ovarian function after Drug-Free IVA?

Summary answer
Physical properties of ovarian cortex in POI are different. A significant ovarian stiffness increase was observed when resumption of ovarian function occurred after Drug-Free IVA.

What is known already
There is increasing evidence that the ovarian extracellular matrix (ECM) plays a critical role in follicle development. Primordial follicles are localized at the collagen-rich ovarian cortex, which offers a rigid physical environment that supports follicular architecture and increases survival. Therefore, premature loss of ovarian function would be associated with a physically less rigid ovarian cortex.

Study design, size, duration
Prospective observational cohort study at a tertiary-care university hospital including POI patients according ESHRE criteria who underwent Drug-Free IVA by laparoscopy between January 2018 and December 2019 and were follow-up for a year after intervention.

Participants/materials, setting, methods
Nineteen patients were included. Resumption of ovarian function was defined as resumption of menstrual cycles (at least three consecutive episodes of menstrual bleeding) and/or the presence of follicles &gt; 10 mm on ultrasound. A sample of ovarian cortex taken during the intervention was analyzed by atomic force microscopy (AFM) in order to quantitatively measure mechanical properties at the nanometer scale (Young’s elastic modulus, E).

Main results and the role of chance
Median data at intervention were: age = 35 years (28–39); length of amenorrhea = 2 years (1–10); FSH level = 104.4 mIU/ml (38.9–176); and AMH = 0.02 ng/ml (0.01–0.1). Resumption of ovarian function was observed in 10 patients (52.6 %), achieving 2 pregnancies (one spontaneous and one after an IVF cycle). Median stiffness (E) measured by AFM was 2583 Pa (999–11296). There were no differences in clinical and hormonal parameters as a function of resumption of ovarian activity. Remarkably, ovarian cortex stiffness was significantly increased in patients with ovarian activity after Dug-Free VIA: 5519 (2260–11296] vs 1501 [999–3474], p value &lt;0.001.

Limitations, reasons for caution
The main limitations of the study are the unavailability of ovarian tissue samples from a control group of patients without POI and the inability to perform histological studies on the same samples in which the biomechanical study was carried out.

Wider implications of the findings
These findings show the high variability of ovarian cortex stiffness in POI and that the increase of this stiffness entails a more favorable status after Drug-Free IVA. This may be related to an ovary with more residual follicles, which would explain a greater chances of ovarian follicular reactivations after treatment.

Trial registration number
not applicable
P-630 Optimal route of progesterone administration for luteal phase support in frozen embryo transfer cycle : systematic review
Authors: Almohammadi A , Black M , Maheshwari A 
Published: June 2022

Abstract

Study question
The optimal route of progesterone administration for luteal phase support in a frozen embryo transfer: A systematic review

Summary answer
Due to the heterogeneity of the results, it was not possible to determine which route had the best outcome.

What is known already
Luteal phase support (LPS) with progesterone is used to optimize outcomes in frozen embryo transfer (FET) cycles, though there is uncertainty about optimal route of progesterone administration.

Study design, size, duration
Objective: To systematically review evidence from randomized controlled trials (RCTs) comparing the various routes (oral, vaginal, and intramuscular) of progesterone administration for LPS in women undergoing FETs.

Participants/materials, setting, methods
In a systematic review of RCTs Medline, Embase, Web of Science and Cochrane controlled trials register were searched without language restrictions from database inception to June 2021. Data were extracted and quality assessed for risk of bias. Individual results were computed as relative risks (RR) with 95% confidence intervals (CI). (Prospective registration, PROSPERO No. CRD4202251017)

Main results and the role of chance
There were 4 eligible trials including 2790 participants. Risk of bias was low concerning randomization process, but there were concerns with respect to intended interventions, missing data, outcome measurement and selective reporting. Interventions were heterogeneous with respect doses and types of progesterone. Only one study (at high risk of bias) showed a statistically significant result in favour of vaginal vs oral progesterone for clinical pregnancy rates (RR 0.45, 95% CI 0.22-0.92); for other route comparisons, individual results were not statistically significant.

Limitations, reasons for caution
The comparison of route of administration of progesterone for LPS needs robust multicentre trials, and advanced data syntheses including indirect comparisons and network meta-analyses in the future.

Wider implications of the findings
The assessment of progesterone supplemention needs a universal agreed protocol to be implemented in guideline for clincian to follow

Trial registration number
Not applicable