bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of biochar on mycorrhizal fungi mediated nutrient uptake by coconut (Cocos nucifera L.) seedlings grown on a Sandy Regosol
Authors: Nirukshan Gertrude Scynthya , Ranasinghe Sanathanie , Sleutel Steven 
Published: December 2022

AbstractBiochar amendment of soil may ameliorate inherently infertile soils, such as in the typical coconut (Cocos nucifera L.) growth areas along tropical coasts, where, moreover, temporary moisture stress commonly occurs. We conducted a pot experiment to evaluate the effects of biochar soil amendment (1% w/w) produced from Gliricidia sepium stems (BC-Gly) and rice husks (BC-RiH) on the growth of coconut seedlings and on N and P uptake mediated by mycorrhizae under wet or dry conditions in a Sandy Regosol. The pots were divided into root and hyphal zones by a nylon mesh, where 15N labelled N and P nutrients were only provided in the hyphal zone. Under wet conditions, biochar application did not affect plant growth, while under dry conditions, the BC-Gly increased root and plant growth similar to that under wet conditions. BC-Gly increased the acidic pH of the soil to a neutral level, and the microbial community shifted towards a higher fungal abundance. The P accumulated (Pacc) in roots was higher with BC-Gly and BC-RiH under dry and wet conditions, respectively. Pacc weakly correlated with the abundance of arbuscular mycorrhizal fungi (AMF) in the hyphal zone. With BC-Gly roots showed lower N derived from fertilizer. We conclude that biochar application has no impact on crop growth under wet conditions, while under dry conditions, BC-Gly stimulates crop growth and P uptake, probably through liming induced P availability but also possibly by some enhancement of AMF growth. The shift in the fungal-oriented microbial community and reduced plant fertilizer N uptake suggested that BC-Gly acted as an additional N source.

Graphical Abstract
The genome loading model for the origin and maintenance of sex in eukaryotes
Authors: Tóth András , Székvölgyi Lóránt , Vellai Tibor 
Published: December 2022

AbstractUnderstanding why sexual reproduction—which involves syngamy (union of gametes) and meiosis—emerged and how it has subsisted for millions of years remains a fundamental problem in biology. Considered as the essence of sex, meiotic recombination is initiated by a DNA double-strand break (DSB) that forms on one of the pairing homologous chromosomes. This DNA lesion is subsequently repaired by gene conversion, the non-reciprocal transfer of genetic information from the intact homolog. A major issue is which of the pairing homologs undergoes DSB formation. Accumulating evidence shows that chromosomal sites where the pairing homologs locally differ in size, i.e., are heterozygous for an insertion or deletion, often display disparity in gene conversion. Biased conversion tends to duplicate insertions and lose deletions. This suggests that DSB is preferentially formed on the “shorter” homologous region, which thereby acts as the recipient for DNA transfer. Thus, sex primarily functions as a genome (re)loading mechanism. It ensures the restoration of formerly lost DNA sequences (deletions) and allows the efficient copying and, mainly in eukaryotes, subsequent spreading of newly emerged sequences (insertions) arising initially in an individual genome, even if they confer no advantage to the host. In this way, sex simultaneously repairs deletions and increases genetic variability underlying adaptation. The model explains a remarkable increase in DNA content during the evolution of eukaryotic genomes.
Genetic architecture and molecular regulation of sorghum domestication
Authors: Ge Fengyong , Xie Peng , Wu Yaorong , Xie Qi 
Published: December 2022

AbstractOver time, wild crops have been domesticated by humans, and the knowledge gained from parallel selection and convergent domestication-related studies in cereals has contributed to current techniques used in molecular plant breeding. Sorghum (Sorghum bicolor (L.) Moench) is the world’s fifth-most popular cereal crop and was one of the first crops cultivated by ancient farmers. In recent years, genetic and genomic studies have provided a better understanding of sorghum domestication and improvements. Here, we discuss the origin, diversification, and domestication processes of sorghum based on archeological discoveries and genomic analyses. This review also comprehensively summarized the genetic basis of key genes related to sorghum domestication and outlined their molecular mechanisms. It highlights that the absence of a domestication bottleneck in sorghum is the result of both evolution and human selection. Additionally, understanding beneficial alleles and their molecular interactions will allow us to quickly design new varieties by further de novo domestication.
Policies and practices of climate change education in South Asia: towards a support framework for an impactful climate change adaptation
Authors: Mbah Marcellus F. , Shingruf Ayesha , Molthan-Hill Petra 
Published: December 2022

AbstractSouth Asia is one of the most vulnerable regions to the impacts of climate change. While it has contributed the least to global greenhouse gas emissions, the people in the region face imminent threats to their health and well-being due to extreme weather events and a range of other climate-induced challenges, such as biodiversity loss and monsoon floods. Climate change education (CCE), if properly implemented, can prove to be vital in addressing such challenges and in helping people make informed decisions on adaptation strategies. To this end, this paper explores how CCE is addressed in the climate policies of South Asian countries. The paper also explores the implementation and operationalization of these policies within the respective countries. A content analysis of the climate policies of member states was conducted for the period 2007–2021. The findings revealed that climate change education has been highlighted within individual policies pertaining to formal and informal education, research and development, and public awareness initiatives. However, a gap analysis between policies and practices reveals that significant work is still needed for the effective implementation of policies supporting CCE at local levels. The current study seeks to contextualize these gaps by situating them within the relevant theoretical backdrops. In doing so, the research aims to make a meaningful contribution to the understanding of how CCE can be effectively deployed and delivered towards an impactful climate change adaptation, building on a support framework.Graphical Abstract
High angle mass flux of an electrospray plume
Authors: Collins Adam L. , Wright Peter L. , Uchizono Nolan M. , Wirz Richard E. 
Published: December 2022

AbstractHigh-resolution mass flux measurements of an electrospray plume are reported at high (>30$$^{\circ }$$

∘

) angles, which are relevant to direct impingement of downstream electrodes. Interrogation of the plume edge greatly reduces uncertainty in electrospray device lifetime estimation related to mass flux to electrode surfaces. An angularly-actuated Thermoelectric Quartz Crystal Microbalance (TQCM) provides resolution down to $$\sim$$
∼
2 pg cm$$^{-2}$$

-
2

s$$^{-1}$$

-
1

, allowing the highest resolution mass flux measurements of an electrospray plume to be reported herein. In-situ microscopy of the electrospray meniscus revealed changes to the electrode lines-of-sight of approximately 2°–3° due to the increasing meniscus tip height with beam current. Using the TQCM measurements and previous QCM results, a data-driven model is proposed for estimating electrode impingement as a function of beam current and aperture line-of-sight, which quantitatively captures the rapid increase in mass flux at higher beam currents or at low angles. The results show it is possible to guarantee negligible electrode impingement within a specified range of throttle levels.
Credibility, transparency, and sustainability in fashion: a game-theoretic perspective
Authors: Nandkeolyar Olivia , Chen Frederick 
Published: December 2022

AbstractWe present a game-theoretic model of a firm’s production decision to analyze the conditions under which the firm would engage in sustainable practices when there exists a certification agency that can audit the firm. Our results show that when the certification agency is firm-owned or when it is an independent, profit-maximizing entity, then there is no equilibrium in which the firm chooses to produce in a sustainable manner. We also present real-world examples from the apparel and footwear industry, as well as the mining industry, that are consistent with our theoretical results. We consider what would happen if the certification agency is government-operated or a non-profit organization with a mandate to monitor the firm’s production process. We show that – combined with tax incentives or subsidies for the firm if necessary, and greater specificity regarding what is sustainable – there exists an equilibrium in which the firm would choose to engage in sustainable production in this case. We analyze extensions of the model to examine conditions under which the phenomenon of greenwashing can arise as an equilibrium outcome. We also propose a “bounty system” that the government can implement to incentivize monitoring of firms’ production processes, and we show how such a policy can induce more sustainable production practices by the firm.
ISOMORPHISM OF LOCALLY COMPACT POLISH METRIC STRUCTURES
Authors: MALICKI MACIEJ 
Published: December 2022

AbstractWe study the isomorphism relation on Borel classes of locally compact Polish metric structures. We prove that isomorphism on such classes is always classifiable by countable structures (equivalently: Borel reducible to graph isomorphism), which implies, in particular, that isometry of locally compact Polish metric spaces is Borel reducible to graph isomorphism. We show that potentially
$\boldsymbol {\Pi }^{0}_{\alpha + 1}$
isomorphism relations are Borel reducible to equality on hereditarily countable sets of rank
$\alpha $
,
$\alpha \geq 2$
. We also study approximations of the Hjorth-isomorphism game, and formulate a condition ruling out classifiability by countable structures.
La estructura del cenáculo: Las redes intelectuales ante la literatura latinoamericana
Authors: Rodríguez-Alfonso Adriana 
Published: December 2022

ResumenEl siguiente artículo analiza el estado actual de la perspectiva de redes intelectuales, especialmente en el cruce con los estudios literarios en América Latina. Partiendo de sus primeros trabajos, discute las mentadasestabilidadytransdisciplinariedaddel campo de estudio, proponiendo un corpus de referencia para las estructuras de sociabilidad literaria. Posteriormente, el artículo revisa críticamente los tópicos, métodos y restricciones, así como las actuales indeterminaciones conceptuales y metodológicas, que sugieren que este es aún un campo en construcción. Finalmente, se proponen algunas ideas novedosas que podrían contribuir al desarrollo interdisciplinario del campo, extendiendo las potencialidades y ventajas de esta perspectiva a los estudios de literatura latinoamericana.
Landscape response to hydroclimate variability shown by the post-Bonneville Flood (ca. 18 ka) fluvial-geomorphic history of the middle Snake River, Idaho, USA
Authors: Bacon Steven N. , Bullard Thomas F. , Kimball Vaughn , Neudorf Christina M. , Baker Shane A. 
Published: December 2022

AbstractThe fluvial geomorphology and stratigraphy on the middle Snake River at Bancroft Springs, Idaho, provide evidence for numerous episodes of Snake River aggradation and incision since the Bonneville Flood at ca. 18 ka. A suite of seven terraces ranging from 20–1 m above modern bankfull elevation records multiple cut-and-fill cycles during the latest Pleistocene and Holocene in response to local base-level controls, variations in sediment supply, and hydroclimate change. Radiocarbon and luminescence dating show that the ages of fluvial aggradation generally coincide with increased sediment supply and likely wetter hydroclimate during onset of the Younger Dryas stadial (ca. 13.2 ka), deglaciation and termination of the Younger Dryas stadial (ca. 11.3 ka), Early Holocene cooling (ca. 8.8 ka), and Neoglacial (ca. 4.5, 2.9, 1.1 ka). Six intervening periods of incision and channel stability may also reflect either reduced sediment supply, drier hydroclimate, or both. The terrace chronology can be correlated to a variety of local and regional paleoclimate proxy records and corresponds well with periods of continental- and global-scale rapid climate change during the Holocene. The fluvial record demonstrates the geomorphic response and sensitivity of large river systems to changes in hydroclimate variability, which has important implications for inferring paleoenvironmental conditions in the region.
Nanoformulation approach for improved antimicrobial activity of bovine lactoperoxidase
Authors: Singh Anamika , Singh Jiya , Singh Tej P. , Sharma Pradeep , Sharma Sujata 
Published: December 2022

AbstractLactoperoxidase (LPO) is a glycosylated antimicrobial protein present in milk with a molecular mass of 78 kDa. LPO is included in many biological processes and is well-known to have biocidal actions, acting as an active antibiotic and antiviral agent. The wide spectrum biocidal activity of LPO is mediated via a definite inhibitory system named lactoperoxidase system which plays a potent role in the innate immune response. With the current advancement in nanotechnology, nanoformulations can be developed for stabilizing and potentiating the activity of LPO for several applications. In the research described in this Research Communication, fresh LPO purified from bovine mammary gland secretions was used for nanoparticle synthesis using a simple thermal process at different pH and temperatures. The round-shaped nanoparticles (average size 229 nm) were successfully synthesized at pH 7.0 and a temperature of 75°C. These nanoparticles were tested against four different bacterial species namely S. flexineri, P. aeruginosa, S. aureus, and E. coli. The prepared nanoparticles exhibited strong inhibition of the growth against all four bacterial species as stated by their MIC and ZOI values. These results may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO in drug discovery and industrial processes.
La isla de Atitlán: un nuevo yacimiento de obsidiana en el Occidente de México
Authors: Blanco Morales Ericka Sofía , Acosta Ochoa Guillermo , Esparza López Rodrigo 
Published: December 2022

ResumenDurante la época prehispánica, la obsidiana se caracterizó por ser una materia prima de primera necesidad para la elaboración de gran variedad de objetos. La tecnología de talla para la extracción de diversos artefactos fue una importante actividad económica. En el occidente de México, la obsidiana resultó un recurso fundamental debido a su abundancia y a la diversidad de yacimientos presentes. Sin embargo, no todos los vidrios volcánicos disponibles tienen las características idóneas para la talla especializada. En la región Valles, dentro de las cuencas centrales del estado de Jalisco, se encuentran dispersos nódulos de obsidiana, con un alto grado de inclusiones, que ha sido referida meramente como “obsidiana de baja calidad”. Hasta ahora, en pocas ocasiones se le ha dado la importancia debida en cuanto al estudio de su composición geoquímica, a pesar de que este sirva para contrastarlo con aquellas que sí fueron empleadas como materia prima. El siguiente trabajo busca establecer las características de la obsidiana disponible en el sitio Atitlán, ubicado en la antigua Cuenca de Magdalena, para compararla con los desechos de un espacio en el que son evidentes talleres especializados de talla intensiva para la extracción de láminas que sirvieron para elaborar piezas útiles. Entre los objetivos primordiales del artículo, es comparar sus particularidades con las de la obsidiana empleada en el proceso productivo proveniente del sitio La Joya. Gracias a esta investigación, demostramos que la obsidiana disponible en la isla no fue aprovechada debido al tamaño de los nódulos disponibles y al alto grado de porosidad e inclusiones.
DNA methylation and gene expression analysis in adipose tissue to identify new loci associated with T2D development in obesity
Authors: Baca Paulina , Barajas-Olmos Francisco , Mirzaeicheshmeh Elaheh , Zerrweck Carlos , Guilbert Lizbeth , Sánchez Ernesto Carlos , Flores-Huacuja Marlen , Villafán Rafael , Martínez-Hernández Angélica , García-Ortiz Humberto , Contreras-Cubas Cecilia , Centeno-Cruz Federico , Orozco Lorena 
Published: December 2022

AbstractBackgroundObesity is accompanied by excess adipose fat storage, which may lead to adipose dysfunction, insulin resistance, and type 2 diabetes (T2D). Currently, the tendency to develop T2D in obesity cannot be explained by genetic variation alone—epigenetic mechanisms, such as DNA methylation, might be involved. Here, we aimed to identify changes in DNA methylation and gene expression in visceral adipose tissue (VAT) that might underlie T2D susceptibility in patients with obesity.MethodsWe investigated DNA methylation and gene expression in VAT biopsies from 19 women with obesity, without (OND = 9) or with T2D (OD = 10). Differences in genome-scale methylation (differentially methylated CpGs [DMCs], false discovery rate < 0.05; and differentially methylated regions [DMRs],pvalue < 0.05) and gene expression (DEGs,pvalue <0.05) between groups were assessed. We searched for overlap between altered methylation and expression and the impact of altered DNA methylation on gene expression, using bootstrap Pearson correlation. The relationship of altered DNA methylation to T2D-related traits was also tested.ResultsWe identified 11 120 DMCs and 96 DMRs distributed across all chromosomes, with the greatest density of epigenomic alterations at the MHC locus. These alterations were found in newly and previously T2D-related genes. Several of these findings were supported by validation and extended multi-ethnic analyses. Of 252 DEGs in the OD group, 68 genes contained DMCs (n = 88), of which 24 demonstrated a significant relationship between gene expression and methylation (pvalues <0.05). Of these, 16, includingATP11A,LPLandEHD2also showed a significant correlation with fasting glucose and HbA1c levels.ConclusionsOur results revealed novel candidate genes related to T2D pathogenesis in obesity. These genes show perturbations in DNA methylation and expression profiles in patients with obesity and diabetes. Methylation profiles were able to discriminate OND from OD individuals; DNA methylation is thus a potential biomarker.
Venetoclax with decitabine versus decitabine monotherapy in elderly acute myeloid leukemia: a propensity score-matched analysis
Authors: Kwag Daehun , Cho Byung-Sik , Bang Su-Yeon , Lee Jong Hyuk , Min Gi-June , Park Sung-Soo , Park Silvia , Yoon Jae-Ho , Lee Sung-Eun , Eom Ki-Seong , Kim Yoo-Jin , Lee Seok , Min Chang-Ki , Cho Seok-Goo , Lee Jong Wook , Kim Hee-Je 
Published: December 2022

AbstractVenetoclax (VEN) combined with azacitidine (AZA) or decitabine (DEC) has been approved for older adults with acute myeloid leukemia (AML) unfit for intensive chemotherapy based on the pivotal VIALE-A trial. However, this trial only compared AZA + VEN with AZA monotherapy. Therefore, we compared the outcomes of consecutive older adults (65 years or older) with newly diagnosed AML who received DEC (n = 230) or DEC + VEN (n = 74) after propensity score matching to construct a one-to-one matched cohort by the nearest neighbor algorithm. The median overall survival was longer in the DEC + VEN group than in the DEC group (13.4 months vs. 8.3 months, p = 0.01). The median event-free survivals were 8.6 and 5.8 months in the DEC + VEN and DEC groups, respectively (p = 0.02). The response rate (complete response, complete response with incomplete hematologic recovery, and morphologic leukemia-free state) was significantly higher in the DEC + VEN group than in the DEC group (70.3% vs. 24.3%, p < 0.01). The 30-day (2.7% vs. 9.5%, p = 0.17) and 60-day (9.5% vs. 18.9%, p = 0.16) mortality rates did not differ between the two groups, nor did the median hospitalization and transfusion rates (hospitalization: 23 days vs. 21 days, p = 0.20; red blood cells: 3.2 units/month vs. 3.5 units/month, p = 0.73; platelets: 2.7 units/month vs. 2.3 units/months, p = 0.48). Of those who received DEC + VEN and became leukemia-free, 29% underwent allogeneic stem cell transplantation and had excellent survival outcomes (one-year survival: 79.4%; one-year non-relapse mortality: 13.3%). This study is the first to provide real-world evidence that DEC + VEN has superior outcomes to DEC monotherapy.
Endoplasmic reticulum stress-mediated cell death in liver injury
Authors: Zhang Jian , Guo Jiafu , Yang Nannan , Huang Yan , Hu Tingting , Rao Chaolong 
Published: December 2022

AbstractThe endoplasmic reticulum is an important intracellular organelle that plays an important role in maintaining cellular homeostasis. Endoplasmic reticulum stress (ERS) and unfolded protein response (UPR) are induced when the body is exposed to adverse external stimuli. It has been established that ERS can induce different cell death modes, including autophagy, apoptosis, ferroptosis, and pyroptosis, through three major transmembrane receptors on the ER membrane, including inositol requirement enzyme 1α, protein kinase-like endoplasmic reticulum kinase and activating transcription factor 6. These different modes of cell death play an important role in the occurrence and development of various diseases, such as neurodegenerative diseases, inflammation, metabolic diseases, and liver injury. As the largest metabolic organ, the liver is rich in enzymes, carries out different functions such as metabolism and secretion, and is the body’s main site of protein synthesis. Accordingly, a well-developed endoplasmic reticulum system is present in hepatocytes to help the liver perform its physiological functions. Current evidence suggests that ERS is closely related to different stages of liver injury, and the death of hepatocytes caused by ERS may be key in liver injury. In addition, an increasing body of evidence suggests that modulating ERS has great potential for treating the liver injury. This article provided a comprehensive overview of the relationship between ERS and four types of cell death. Moreover, we discussed the mechanism of ERS and UPR in different liver injuries and their potential therapeutic strategies.
A comparison of the genes and genesets identified by GWAS and EWAS of fifteen complex traits
Authors: Battram Thomas , Gaunt Tom R. , Relton Caroline L. , Timpson Nicholas J. , Hemani Gibran 
Published: December 2022

AbstractIdentifying genomic regions pertinent to complex traits is a common goal of genome-wide and epigenome-wide association studies (GWAS and EWAS). GWAS identify causal genetic variants, directly or via linkage disequilibrium, and EWAS identify variation in DNA methylation associated with a trait. While GWAS in principle will only detect variants due to causal genes, EWAS can also identify genes via confounding, or reverse causation. We systematically compare GWAS (N > 50,000) and EWAS (N > 4500) results of 15 complex traits. We evaluate if the genes or gene ontology terms flagged by GWAS and EWAS overlap, and find substantial overlap for diastolic blood pressure, (gene overlap P = 5.2 × 10−6; term overlap P = 0.001). We superimpose our empirical findings against simulated models of varying genetic and epigenetic architectures and observe that in most cases GWAS and EWAS are likely capturing distinct genesets. Our results indicate that GWAS and EWAS are capturing different aspects of the biology of complex traits.