bluuebunny/crossref_metadata_2025_split huggingface dataset:
PIK3CA mutations in breast cancer
Authors: Reinhardt Kristin , Stückrath Kathrin , Kaufhold Sandy , Uleer Christoph , Hanf Volker , Lantzsch Tilmann , Peschel Susanne , John Jutta , Pöhler Marleen , Bauer Marcus , Bürrig Friedrich Karl , Weigert Edith , Buchmann Jörg , Kantelhardt Eva Johanna , Thomssen Christoph , Vetter Martina 
Published: April 2022

Abstract
Purpose: The phosphatidylinositide-3-kinase (PI3K) pathway regulates intracellular processes in relation to proliferation and apoptosis, which can be activated by somatic PIK3CA mutations. The aim of this work was to analyze the prevalence of PIK3CA mutations in an unselected cohort of patients with early stage breast cancer and their association with survival.Patients and methods: From a prospective, multicentre cohort of 1,270-breast cancer patients (PiA, Prognostic Assessment in routine application, NCT 01592825) 1,123 tumors were tested for PIK3CA-mutations in three hotspots C775 (H1047R), C763 (E545K) and C760 (E542K) by quantitative PCR. The primary objectives were the prevalence of somatic PIK3CA mutations and the associations between PIK3CA mutations and clinical and histopathological parameters. Secondary objectives were the association of PIK3CA mutations with recurrence free interval (RFI) and overall survival.Results: The overall PIK3CA mutation rate was 26.7% (300 out of 1,123). PIK3CA mutations were significantly more frequent in tumors with more favorable factors like steroid hormone receptor (HR)-positive and HER2-negative (31.4%), and G1/ G2 tumours (32.8%). We found a significant detrimental prognostic impact of PIK3CA-mutations on RFI for patients with HR-positive BCs who had only aromatase inhibitors as adjuvant therapy (adjusted HR 3.71, 95% CI 1.24-11.11), whereas PIK3CA mutations in HR-negative BCs were insignificantly associated with improved RFI (adjusted HR=0.43; 95% CI 0.10-1.82).Conclusion: The impaired prognosis for HR-positive BCs and concurrent PIK3CA mutations supports the hypothesis that the activation of PI3K might lead to endocrine resistance. Conversely, patients with HR-negative tumors and PIK3CA mutations might benefit from chemotherapy compared to patients with wildtype PIK3CA gene.
The circular RNA 0010117 promotes the aggressive behavior of glioblastoma by regulating miRNA-6779-5p/SPEN axis
Authors: Yang Xuanyong , Liu Yue , Zhou Xinhui , Chen Kang , Xu Jiang , Xu Shan 
Published: April 2022

Abstract
Background: Noncoding RNAs (ncRNAs) play important roles in cancer biology, providing potential targets for cancer intervention. As a new class of endogenous noncoding RNAs, circular RNAs (circRNAs) have been recently identified in cell development and function, and certain types of pathological responses, to contribute to cancer progression including glioblastoma. However, the potential mechanisms underlying the relationship between circRNAs and glioblastoma progression are still largely unknow. Methods: The expression and roles of circular RNA 0010117 (circ-0010117) were examined in vitro and in vivo. Quantitative RT-PCR and western blotting were used to measure the expression of circ RNA, miRNA, each gene, or relative proteins. Cell biology experiments were performed to detect the biological function of circ-0010117 in glioblastoma cell lines. Moreover, bioinformatics analysis, luciferase reporter assay, and functional complementation analysis were carried out to investigate the target gene. Tumorigenesis was also evaluated by xenografting cells into nude mice. Results: In this study, we find that circ-0010117 is down-regulated in glioblastoma compared with corresponding paratumoral tissues. Subsequently, we observe that circ-0010117 can regulate aggressiveness in glioblastoma cells through miR-6779-5p. Furthermore, SPEN is verified as a direct target of miR-6779-5p and also contributes to circ-0010117 regulatory network. In addition, we identify that overexpression of circ-0010117 can suppresses tumorigenesis in nude mice. Conclusion: Our findings indicate that the circular RNA 0010117 promotes the aggressive behavior of glioblastoma by regulating miRNA-6779-5p/SPEN axis. And our results provide the rationale for the use of circ-0010117 as a novel potential therapeutic target in glioblastoma.
Study of the Accuracy of a Radial Arterial Pressure Waveform Cardiac Output Measurement Device After Cardiac Surgery
Authors: Ordoñez-Rufat Pilar , Fora Núria Mancho , Cordomí Cristian Tebé , Martínez Victoria Polit , Lencina Ricardo Abellán Abellán , Álvarez Joaquin Fernandez Fernandez , Esteve-Urbano Francisco , Lopez-Delgado Juan Carlos 
Published: April 2022

Abstract
Purpose: To evaluate the accuracy of ProAQT® sensor in patients who underwent cardiac surgery. Less-invasive monitoring, such as arterial pulse contour analysis (ProAQT® sensor), represents an alternative when hemodynamic monitoring is necessary for guiding postoperative resuscitation but invasive monitoring is not technically possible. Methods: Prospective observational study in a Surgical Intensive Care Unit of a tertiary university hospital. Ten patients who underwent cardiac surgery were included. We compared cardiac index (CI) measured simultaneously by ProAQT® sensor (i.e., radial arterial pressure waveform) with pulmonary arterial catheter (PAC). Parameters were assessed at predefined time points during early postoperative courses (6h, 12h, 24h, 48h and 72h). The degree of agreement with CI between PAC and ProAQT® sensors across time points was measured by the concordance correlation coefficient, Bland-Altman and four-quadrant plot analyses.Results: Sixty-three pairs of measurements were analyzed. We showed that measurements of CI were slightly higher with PAC (β ̂=-0.146, p-value=0.094). The concordance correlation coefficient for the additive model of CI was 0.64 (95% Confidence Interval: 0.36, 0.82), indicating a high concordance between both sensors. Bland-Altmann analysis showed a mean bias of 0.45 L·min-1·m-2, limits of agreement from -1.65 to 2.3 L·min-1·m-2, and percentage of error was 82.5%. Four-quadrant plot of changes in CI showed a good concordance rate (75%), which increases after applying the exclusion zone (87%).Conclusion: In patients undergoing cardiac surgery, the ProAQT® sensor may be useful to monitor CI and it may provide a reliable estimate of its absolute value during postoperative period.
Intraseasonal variability of global land monsoon precipitation and its recent trend
Authors: Liu Fei , Wang Bin , Ouyang Yu , Wang Hui , Qiao Shaobo , Chen Guosen , Dong Wenjie 
Published: April 2022

Abstract

Accurate prediction of global land monsoon rainfall on a sub-seasonal (2-8 weeks) time scale has become a worldwide demand. Current forecasts of weekly-mean rainfall in most monsoon regions, however, have limited skills beyond two weeks, calling for a more profound understanding of monsoon intraseasonal variability (ISV). We show that the high-frequency (HF; 8-20 days) ISV, crucial for the Week 2 and Week 3 predictions, accounts for about 53-70% of the total (8-70 days) ISV, generally dominating the sub-seasonal predictability of various land monsoons, while the low-frequency (LF; 20-70 days)’s contribution is comparable to HF only over Australia (AU; 47%), South Asia (SA; 43%), and South America (SAM; 40%). The leading modes of HFISVs in Northern Hemisphere (NH) monsoons primarily originate from different convectively coupled equatorial waves, while from mid-latitude wave trains for Southern Hemisphere (SH) monsoons and East Asian (EA) monsoon. The Madden-Julian Oscillation (MJO) directly regulates LFISVs in Asian-Australian monsoon and affects American and African monsoons by exciting Kelvin waves and mid-latitude teleconnections. During the past four decades, the HF (LF) ISVs have considerably intensified over Asian (Asian-Australian) monsoon but weakened over American (SAM) monsoon. Sub-seasonal to seasonal (S2S) prediction models exhibit higher sub-seasonal prediction skills over AU, SA, and SAM monsoons that have larger LFISV contributions than other monsoons. These results suggest an urgent need to improve the simulation of convectively coupled equatorial waves and two-way interactions between regional monsoon ISVs and mid-latitude processes and between MJO and regional monsoons, especially under the global warming scenarios.
Godunov-type solutions for free surface transient flow in pipeline incorporating unsteady friction
Authors: Hu Yinying , Zhou Ling , Pan Tianwen , Fang Haoyu , Li Yunjie , Liu Deyou 
Published: April 2022

AbstractA finite-volume second-order Godunov-type scheme (GTS) combining the unsteady friction model (UFM) is introduced to simulate free surface flow in pipelines. The exact solution to the Riemann problem calculates the mass and momentum fluxes while considering the Brunone unsteady friction factor. One simple boundary treatment with double virtual cells is proposed to ensure the whole computation domain with second-order accuracy. Results of various transient free-surface flows achieved by the proposed models are compared with exact solution, experimental data, the four-point implicit Preissmann scheme solution, as well as predictions by the classic Method of Characteristics (MOC). Results show that the proposed second-order GTS UFMs are accurate, efficient, and stable even for Courant numbers less than one and sparse grid. The four-point implicit Preissmann scheme may produce severe numerical attenuation in the case of large time steps and unsuitable weighting factors, while the MOC scheme may produce severe numerical attenuation in the case of a low Courant number and could not maintain mass conservation. The numerical simulations considering the unsteady friction factor are closer to the measured water depth variations. The effect of unsteady friction becomes more important as the initial water depth difference increases significantly.
Multi-step prediction of dissolved oxygen in rivers based on random forest missing value imputation and attention mechanism coupled with recurrent neural network
Authors: Huan Juan , Li Mingbao , Xu Xiangen , Zhang Hao , Yang Beier , Jianming Jiang , Shi Bing 
Published: April 2022

Abstract
Accurately predicting dissolved oxygen is of great significance to the intelligent management and control of river water quality. However, due to the interference of external factors and the irregularity of its changes, this is still a ticklish problem, especially in multi-step forecasting. This article mainly studies two issues: we first analyze the lack of water quality data and propose to use the random forest algorithm to interpolate the missing data. Then, we systematically discuss and compare water quality prediction methods based on attention-based RNN, and develop attention-based RNN into a multi-step prediction for dissolved oxygen. Finally, we applied the model to the canal in Jiangnan (China) and compared eight baseline methods. In the dissolved oxygen single-step prediction, the attention-based GRU model has better performance. Its measure indicators MAE, RMSE, and R2 are 0.051, 0.225, and 0.958, which are better than baseline methods. Next, attention-based GRU was developed into multi-step prediction, which can predict the dissolved oxygen in the next 20 hours with high prediction accuracy. The MAE, RMSE, and R2 are 0.253, 0.306, and 0.918. Experimental results show that attention-based GRU can achieve more accurate dissolved oxygen prediction in single-neural network and multi-step predictions.
Recycling iron from pickling sludge to activate peroxydisulfate for the degradation of phenol
Authors: Yang Shaopeng , Wang Dongzhe , Xue Gang , Wang Li , Duan Xinhui , Gong Cairong 
Published: April 2022

Abstract
In this work, iron was recovered from a kind of iron-rich pickling sludge by an acid leaching process, and the recycled iron was used as a catalyst to activate peroxydisulfate (PS) for the degradation of phenol. Different kinds of sludge catalysts were prepared by different drying methods such as ordinary drying (Ods), freeze drying (Fds) and vacuum drying (Vds). The degradation performance of the different catalysts/PS system under different conditions was explored, the vacuum drying sludge catalyst (Vds) has the best activity in a wide pH range (pH = 3–10) and a wide temperature range (0–40 °C). At the same time, the effect of a series of chelating agents (Oxalic acid (OA), Citric acid (CA), Tartaric acid (TA), Malic acid (DL-MA) and Ethylenediaminetetraacetic acid (EDTA)) on Vds/PS system was verified, and TA was selected as the best chelating agent to promote the degradation of the second stage where the degradation rate is limited. The quenching experiment and electron paramagnetic resonance (EPR) analysis indicated that hydroxyl radical (·OH) and sulfate radical (SO4̇−) were responsible for the abatement of the organic contaminant with ·OH playing a more important role. In summary, this study proposed an environmentally-friendly approach for the application of iron-rich pickling sludge in the remediation of phenol-contaminated water.
Advances and Prospects of Ultrasound Targeted Drug Delivery Systems Using Biomaterial-modified Micro/Nanobubbles for Tumor Therapy
Authors: Lin Chen , Chen Ye-Zi , Wu Bo , Yang Meng-Ting , Liu Chao-Qi , Zhao Yun 
Published: April 2022

Abstract:
The incidence of malignant tumors is rising rapidly and tends to be in the
younger, which has been one of the most important factors endangering the safety of human
life. Ultrasound micro/nanobubbles, as a noninvasive and highly specific antitumor
strategy, can reach and destroy tumor tissue through their effects of cavitation and acoustic
perforation under the guidance of ultrasound. Meanwhile, micro/nanobubbles are now
used as a novel drug carrier, releasing drugs at a target region, especially on the
prospects of biomaterial-modified micro/nanobubbles as a dual modality for drug delivery
and therapeutic monitoring. Successful evaluation of the sonoporation mechanism(s),
ultrasound parameters, drug type, and dose will need to be addressed before translating
this technology for clinical use. Therefore, this paper collects the literature on the experimental
and clinical studies of ultrasound biomaterial-modified micro/nanobubbles therapy
in vitro and in vivo in recent years.
Overcoming Skin Damage from Pollution via Novel Skincare Strategies
Authors: Charitakis Alexandros , Assi Sulaf , Yousaf Sakib , Khan Iftikhar 
Published: April 2022

Abstract:
Urban pollution is one of the main problems encountered worldwide, with a major impact on public
health as well as the environment. The health impact of urban pollution is not limited to respiratory conditions
but also encompasses major skin problems, including irritation, skin ageing, and skin cancer. Toxic gases and
particulate matter are the main pollutants that exhibit extensive local variability. The aforementioned pollutants
are small particles that attach to the skin or penetrate it, enhancing free radicals’ production inside the inner skin
layers. This urges the need to propose cosmetic products that help prevent and/or minimise pollutants’ effects
on the skin, whether irritation, ageing, and cancer. Furthermore, intrinsic and extrinsic factors contribute to skin
irritation and ageing. Intrinsic factors are within skin factors and include genetic and physiological characteristics
of individuals. Moreover, extrinsic factors comprise environmental factors such as humidity, temperature,
and smoke. Subsequently, active ingredients with anti pollutant properties addressed the intrinsic and extrinsic
factors by four mechanisms: free radical neutralisation, film-forming ability, skin barrier enhancement, and fortification.
Such ingredients include vitamin A derivatives, vitamin C derivatives, carbohydrates, and plantbased
products. Yet, very limited studies have evaluated the effectiveness of the aforementioned active ingredients
against irritation or ageing, which should be considered in future work.
Curcumin Prevents the Glycation of Tricarboxylic Acid Cycle and Cell Respiration
Proteins in the Heart of Mice Fed with a High-fructose Diet
Authors: León-García María Cristina , Silva-Gaona Oscar Gerardo , Hernández-Ortiz Magdalena , Vargas-Ortiz Katya , Ramírez-Emiliano Joel , Garay-Sevilla Ma Eugenia , Encarnación-Guevara Sergio , Pérez-Vázquez Victoriano 
Published: April 2022

Background:
A high fructose diet (HFD) induces protein glycation. The latter is related to a higher
risk of cardiovascular disease. Curcumin is a natural pleiotropic compound that may possess antiglycant properties.

Objective:
The study aims to analyze the effect of curcumin on the content of glycated proteins in the hearts of
6-week-old mice fed with a HFD for 15 weeks.

Methods:
Mice were allocated into four groups (n = 6/group): a control group that received a standard diet
(CT); a group that received 30% w/v fructose in water (F); a group that received 0.75% w/w curcumin supplemented
in food (C); a group that received 30% w/v fructose in water and 0.75% w/w curcumin supplemented in
food (F+C). The content of glycated proteins in the heart was determined by Western Blot (whereas the spots
were detected by 2D-PAGE) using anti-AGE and anti-CML antibodies. Densitometric analysis was performed
using the ImageLab software. Glycated proteins were identified by MALDI-TOF-MS, and an ontological analysis
was performed in terms of biological processes and molecular function based on the STRING and DAVID
databases.

Results:
Fourteen glycated protein spots were detected, two of them with anti-AGE and the other 12 with anti-
CML. In total, eleven glycated proteins were identified, out of which three had decreased glycation levels due
to curcumin exposure. The identified proteins participate in processes such as cellular respiration, oxidative
phosphorylation, lipid metabolism, carbohydrate metabolism, the tricarboxylic acid cycle (TAC), and the organization
of intermediate filaments.

Conclusions:
Curcumin decreased the fructose-induced glycation level of the ACO2, NDUFS7, and DLAT proteins.
From Elusive Monomeric Metaphosphates to Oligomeric Metaphosphate Reagents:
New Avenue to Halogen-free Phosphorylation of Biomolecules
Authors: Romanenko Vadim D. 
Published: April 2022

Abstract:
This perspective highlights a new environmentally benign strategy for the introduction
of phosphate functionality into organic substrates via activated cyclic oligometaphosphates.
The use of these novel phosphorylating reagents greatly simplify the synthesis of nucleoside
mono- and polyphosphates and provides a platform to access diverse phosphorylated
amino acids, peptides, sugars, and other phosphorus-containing fine chemicals. The new phosphorylation
protocol represents an advance in terms of atom-efficiency, regioselectivity, functional
tolerance and safety for the environment.
Functional Ability of Dental Materials After Inclusion of Nanocarriers
Containing Functional Substances: A Systematic Review
Authors: Dutra Borges Boniek Castillo , Costa Moan Jéfter Fernandes , de Oliveira Francisca Jennifer Duarte , Wanderley e Lima Renally Bezerra 
Published: April 2022

Background:
Nanocarriers are nanoparticles used to transport functional substances.

Objective::
This study aimed to perform a systematic review analyzing the functional ability of dental
materials after the inclusion of nanocarriers containing functional substances.

Materials and Methods:
We searched PubMed, Web of Science, EMBASE, SciELO, and Science
Direct from (02/03/21) to (02/06/21) without restriction on the year of publication. We included studies
evaluating the incorporation of Chitosan, PEG-PCL (poly(ethylene glycol)-poly(ε-caprolactone),
PLGA (poly(lactic-co-glycolic acid), and mesoporous silica into dental materials. We analyzed the
risk of bias with CRIS guidelines and consolidated all analyses using Microsoft Office Excel.

Results:
In total, we found 656 studies and included 11. The articles investigated materials to eliminate
microorganisms, interference with materials’ mechanical properties, elution of remineralizing
substances, and reduction of oral anesthetic toxicity. Chitosan and mesoporous silica were the most
prevalent nanocarriers. Among all properties, the antibacterial activity was the most analyzed. The
functional ability of dental materials provided by all nanocarriers improved.

Conclusion:
The use of nanocarriers may be a useful way to produce dental materials with improved
functional abilities. We registered this manuscript in the Open Science Framework platform - DOI
10.17605/OSF.IO/RP5XK, available at https://osf.io/qg49x.
MicroRNAs and Efferocytosis: Implications for Diagnosis and Therapy
Authors: Gheibihayat Seyed Mohammad , Mahmoudi Ali , Moadab Fatemeh , Safdarian Esmat , Navashenaq Jamshid Gholizadeh , Rezaee Mehdi 
Published: April 2022

Abstract:About 10-100 billion cells are generated in the human body in a day, and accordingly, 10- 100 billion cells predominantly die for maintaining homeostasis. Dead cells generated by apoptosis are also rapidly engulfed by macrophages (Mθs) to be degraded. In case of the inefficient engulfment of apoptotic cells (ACs) via Mθs, they experience secondary necrosis and thus release intracellular materials, which display damage-associated molecular patterns (DAMPs) and result in diseases. Over the last decades, researchers have also reflected on the significant contribution of microRNAs (miRNAs) to autoimmune diseases through the regulation of Mθs functions. Moreover, miRNAs have shown intricate involvement with completely adjusting basic Mθs functions, such as phagocytosis, inflammation, efferocytosis, tumor promotion, and tissue repair. In this review, the mechanism of efferocytosis containing "Find-Me", "Eat-Me", and "Digest-Me" signals is summarized and the biogenesis of miRNAs is briefly described. Finally, the role of miRNAs in efferocytosis is discussed. It is concluded that miRNAs represent promising treatments and diagnostic targets in impaired phagocytic clearance, which leads to different diseases.
DNA Nanobots – Emerging Customized Nanomedicine in Oncology
Authors: Deshmukh Rohitas , Singh Rajesh 
Published: April 2022

Abstract:
Cancer is one of the most lethal diseases of the twenty-first century. Many medicines, including
antitumor antibiotics, deliver tedious and severe chemotherapy and radiation treatment, both of
which have significant side effects. DNA nanorobots, as an alternative, might be used as a cancer treatment
method that is both safer and more precise than current treatments. DNA nanobots are being
praised as a major milestone in medical research. The major goal of these nanobots is to find and destroy
malignant cells in the human body. A unique strand of DNA is folded into the systematic form to
create these nanobots. DNA origami has magnified passive tumor-targeting and prolonged properties at
the tumor location. The triangle-like DNA origami, in particular, shows excellent accumulation on passive
targeting of the tumor. Self-built DNA origami nanostructures were utilized to deliver the anticancer
drug doxorubicin into tumors, and the approach was found to be highly successful in vivo. In
another demonstration, a robot was made with the help of DNA origami and aptamer for folding a 90nm
long tube-like apparatus. It was carried out to transport the blood coagulation protease thrombin in the
interior portion guarded against blood plasma protein and circulating platelets. The robot unfolded once
the aptamer was identified and attached to its tumor-specific target molecule, delivering thrombin to the
circulation, stimulating coagulation of the regional malignant cells, and proceeding to tumor necrosis
and tumor growth inhibition. Various studies revealed the effectiveness of DNA nanobots in cancer
therapy.