bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined effect of sarcopenia and osteoporosis on the prevalence of mild cognitive impairment in Chinese community-dwelling old adults
Authors: Sun Yuechao , Zheng Yue , Chen Xiaoyu , Han Peipei , Zhang Xinyue , Meng Shixing , Zhao Chenyang , Xu Che , Zheng Yaqing , Zhang Ziwei , Li Ming , Guo Qi 
Published: October 2022

Abstract
Purpose: The study aimed to explore the single and combined effects of sarcopenia and osteoporosis on the prevalence of mild cognitive impairment (MCI) in a Chinese community-dwelling elderly population.
Patients and methods: A total of 1268 community-dwelling participants aged ≥ 60 in Shanghai of China from 2019 to 2020. The dual-energy X-ray image of forearm bone of each participant whose T score is less than -2.5 is defined as osteoporosis. Sarcopenia was diagnosed according to the 2019 Asian sarcopenia working group (AWGS) standard. The Mini Mental State Examination (MMSE) and the Instrumental Activities of Daily Living (IADL) scales were used to diagnose MCI patients.
Results: This study was conducted on 1,268 (30.4% male) Chinese community-dwelling participants with a mean age of 71.8±5.8 years. The prevalence of MCI was significantly greater in the cooccurring group (OR=3.11, 95% CI=1.88-5.17) than in the sarcopenia alone group (OR=1.80, 95% CI=1.04-3.10) or osteoporosis alone group (OR=1.67, 95% CI=1.16-2.41) in unadjusted model. After adjusting for potential confounding factors, the results remained the same (OR=2.51, 95% CI=1.36-4.64).
Conclusion: In our study, although sarcopenia and osteoporosis did not independently affect the prevalence of MCI, the joint of the osteoporosis and sarcopenia can synergistically increase the prevalence of MCI in Chinese community-dwelling older adults.
HSP90/c-Myc Axis regulated by lncRNA LINC00461 upregulation inhibited the ubiquitination of c-Myc and promotes glucose Metabolism and proliferation through LDHA in Triple-negative Breast Cancer
Authors: Feng Xiulai , Liu HuiFang , Lu Zinan , Qiao Lei , Ma Bin , Guo Wenjia , Sun Gang 
Published: October 2022

Abstract
LINC00461 represents a new long noncoding RNA. However, it is unclear whether LINC00461 is associated with glucose metabolism and proliferation in triple-negative breast cancer. Here, we show that LINC00461 overexpression induces glucose metabolism and proliferation in TNBC, whereas its downregulation markedly reduces glucose metabolism and proliferation. Mechanistically, LINC00461 might function in TNBC by binding with HSP90. Then, it enhances the interaction between HSP90 and c-Myc and inhibits ubiquitination and degradation of c-Myc to regulate c-Myc target genes-LDHA. Clinically, LINC00461 has tight associations with tumor grade and TNM in cancer patients. There is compelling evidence LINC00461 may be exploited as a possible novel molecular marker and therapeutic target in TNBC.
The Ecuadorian extract -BIRM- induces in vitro antitumor effects on CF41.Mg canine mammary carcinoma cells
Authors: Gallmeier Michelle , Guzmán Sofía , Cruz Pamela , Reyes Fernando , Torres Cristian G. 
Published: October 2022

Abstract
Mammary tumors are the most frequent tumor disease in female dogs. Since conventional therapies as surgery and chemo are of partial utility, especially in histological high-grade carcinomas, are non-selective and can trigger potential adverse effects, it is necessary to study new and harmless antitumor treatment alternatives. Commercial herbal extract derived from Kalanchoi gastonis-bonnieri -BIRM- has been used empirically for cancer, however, there are few studies that reveal its antitumor potential. BIRM induces an antiproliferative and pro-apoptotic effect, mainly on androgen-dependent human prostate carcinoma cells. It attractive to analyze whether BIRM also induces antiproliferative effects on CF41.Mg canine mammary carcinoma cells, a cell line representative of high-grade mammary tumors. For this purpose, proliferation of CF41.Mg and non-tumorigenic MDCK cells were studied in the presence of herbal extract. In addition, the effect of BIRM on apoptosis and invasive ability of tumor cells was analyzed. BIRM decreased cell proliferation on both cell types, effects that were concentration dependent. Moreover, the extract induced apoptosis and a decrease in the invasiveness of CF41.Mg cells. In conclusion, BIRM triggers a non-selective antiproliferative effect on CF41.Mg cells, diminishing their invasion ability. These outcomes may support future clinical trials involving this plant extract in dogs with high-grade mammary carcinoma.
Ultrafast, autonomous self-healable iontronic skin exhibiting piezo-ionic dynamics
Authors: Boahen Elvis K. , Pan Baohai , Kweon Hyukmin , Kim Joo Sung , Choi Hanbin , Kong Zhengyang , Zhu Jin , Ying Wu Bin , Lee Kyung Jin , Kim Do Hwan 
Published: October 2022

Abstract
The self-healing properties and ionic sensing capabilities of the human skin offer inspiring groundwork for the designs of stretchable iontronic skins. However, from electronic to ionic mechanosensitive skins, simultaneously achieving autonomously superior self-healing properties, superior elasticity, and effective control of ion dynamics in a homogeneous system is rarely feasible. Here, we report a Cl-functionalized iontronic pressure sensitive material (CLiPS), designed via the introduction of Cl-functionalized groups into a polyurethane matrix, which realizes an ultrafast, autonomous self-healing speed (4.3 µm/min), high self-healing efficiency (91% within 60 min), and mechanosensitive piezo-ionic dynamics. This strategy promotes both an excellent elastic recovery (100%) and effective control of ion dynamics because the Cl groups trap the ions in the system via ion-dipole interactions, resulting in excellent pressure sensitivity (7.36 kPa-1) for tactile sensors. The skin-like sensor responds to pressure variations, demonstrating its potential for touch modulation in future wearable electronics and human–machine interfaces.
Temporal Evolution and Differential Patterns of Cellular Reconstitution after Therapy for Childhood Cancers
Authors: Hofmann Gina , Zierk Jakob , Sobik Bettina , Wotschofsky Zofia , Sembill Stephanie , Krumbholz Manuela , Metzler Markus , Karow Axel 
Published: October 2022

Abstract
The cellular reconstitution after childhood cancer therapy is associated with the risk of infection and efficacy of revaccination. Many studies have described the reconstitution after stem cell transplantation (SCT). The recovery after cancer treatment in children who have not undergone SCT has mainly been investigated in acute lymphoblastic leukemia (ALL), less for solid tumors. Here, we have examined the temporal evolution of total leukocyte, neutrophil and lymphocyte counts as surrogate parameters for the post-therapeutic immune recovery in a cohort of n = 52 patients with ALL in comparison to n = 58 patients with Hodgkin`s disease (HD) and n = 22 patients with Ewing sarcoma (ES). Patients with ALL showed an efficient increase in blood counts reaching the age-adjusted lower limits of normal between 4 and 5 months after the end of maintenance therapy. The two groups of patients with HD and ES exhibited a comparably delayed recovery of total leukocytes due to a protracted post-therapeutic lymphopenia which was most pronounced in patients with HD after irradiation. Our results underline that the kinetics of cellular reconstitution after therapy for HD and ES differ significantly from ALL and depend on treatment regimens and modalities. This suggests a need for disease- and treatment-specific recommendations concerning the duration of infection prophylaxis and the timing of revaccination.
Prevalence of arterial stiffness and factors associated with arterial stiffness in rheumatoid arthritis patients without cardiovascular disease symptoms: a cross- sectional study
Authors: Nilmoje Thanapon , Aiewruengsurat Duangkamol , Tirasathit Pawita , Juthong Siriporn , Hiranyachattada Siriphun , Chongsuvivatwong Virasakdi 
Published: October 2022

Abstract
Asymptomatic peripheral arterial stiffness is an intermediary outcome of atherosclerosis which leads to cardiovascular morbidity and mortality in rheumatoid arthritis (RA) patients. We conducted this study to determine the prevalence of arterial stiffness as measured by cardio-ankle vascular index (CAVI) in RA patients compared to controls, and to assess factors associated with arterial stiffness in RA patients. This stratified cross-sectional study was performed on 48 confirmed RA patients without overt cardiovascular disease and 51 controls matched for age and sex. All subjects underwent CAVI and ankle-brachial index (ABI). Arterial stiffness prevalence measured by CAVI was significantly high in the RA group (18.8%) than that in the control (17.6%). So was the respective difference in mean (SD) 8.2 (1.1) vs 7.7 (1.2). No subject in either group had abnormal ABI. Independent variables associated with high CAVI values included low and moderate disease activity, high age and high systolic blood pressure (SBP). This study may imply cardiovascular benefit to reduce RA disease activity especially among those with existing other cardiovascular risk factor.
Diagnosis of Serious Conditions Delayed in Association with Ondansetron Treatment for Vomiting in the Pediatric Emergency Department
Authors: Soffer Gili Palnizky , Schnapp Zeev , Miroluz Dana , Rimon Ayelet 
Published: October 2022

Abstract
We evaluated the characteristics and sought risk factors for hospitalization in children who return to the emergency department (ED) within 7 days of discharge after oral or intravenous ondansetron treatment for vomiting. The secondary aim was to determine whether the diagnosis of any serious condition had been delayed as the result of discharge after ondansetron treatment. This retrospective analysis of the medical records of children who had been treated for vomiting with ondansetron in a tertiary care pediatric ED and revisited the ED within 7 days was performed between 2017 and 2019. We compared demographic and clinical features as well as management between hospitalized and discharged patients, focusing upon potentially delayed diagnoses of serious conditions. Fifty of the 89 ondansetron treated children (56.2%) who revisited the ED were discharged home after their second ED visit and the remaining 39 (43.8%) were hospitalized. No parameter of the management of the first visit was predictive of the outcome of the revisit. Five revisit patients (5.6%) were newly diagnosed with a serious condition, with intussusception and ovarian torsion being the most substantial time-sensitive delays (the other diagnoses were pneumonia and aseptic meningitis).
Conclusion: Physicians assessing patients who had been treated with ondansetron as supportive care for vomiting at an earlier visit to the pediatric ED should consider alternative diagnoses despite initial clinical improvement. No definitive risk factor for readmission was identified, but a high level of alertness to a possible meningeal or acute abdominal source is imperative.
Overlapping filter-bank convolutional neural network for multisubject multicategory motor imagery BCI
Authors: Luo Jing , Mao Qi , Shi Zhenghao , Ren Xiaorong , Hei Xinhong 
Published: October 2022

Abstract
Background
Recently, CNN-based models have been widely used in motor imagery brain-computer interfaces (BCIs) due to their powerful feature representation ability. However, in multisubject motor imagery BCI, the discriminative frequency bands vary from subject to subject. Thus, using CNNs to extract discriminative features from EEG signals of different frequency components is a promising method in multisubject EEG recognition.
Methods
This paper presents a novel overlapping filter-bank CNN to incorporate discriminative information from multiple frequency components in multisubject motor imagery recognition. Specifically, two overlapping filter banks with fixed low-cut frequency or sliding low-cut frequency are employed to obtain multiple frequency component representations of EEG signals. Then, multiple CNN models are trained separately. Finally, the output probabilities of multiple CNN models are integrated to determine the predicted EEG label.
Results
Experiments were conducted based on three popular CNN backbone models and two public datasets. We compared the performance of overlapping filter-bank CNN with the state-of-the-art methods and traditional nonoverlapping-based CNN, and the results showed that the overlapping filter-bank CNN was efficient and universal in improving multisubject motor imagery BCI performance.
Conclusion
The proposed overlapping filter bank CNN framework with fixed low-cut frequency is an efficient and universal method to improve the performance of multisubject motor imagery BCI.
A randomized clinical trial of innate immunity restoration by branched-chain amino acid granules in cirrhosis patients
Authors: Chamroonkul Naichaya , Rujeerapaiboon Natthapat , Sripong Pimsiri , Kaewdech Apichat , Piratvisuth Teerha 
Published: October 2022

Abstract
Background

Infection among cirrhotic patients results in major morbidity and mortality. Reduction in phagocytic activation, as part of immunoparesis, is a distinctive key component of cirrhosis-associated immune dysfunction (CAID) and predicts the development of infection. However, there are limited data on immunotherapeutic approaches to restore phagocytosis. We aimed to determine the effect of branched-chain amino acid (BCAA) granules on phagocytic activity in patients with CAID.

Method

In this double-blind randomized controlled trial, participants were randomly assigned (1:1 ratio stratified by Child–Pugh status) to receive either BCAA granules or placebo. In the 3rd and 6th months, phagocytic activity was assessed by flow cytometry. The primary endpoint was the restoration of innate immunity at the 6th month, defined as ≥ 75% phagocytic activity; the secondary endpoints were the accretion of phagocytic activity and hospitalization due to infection.

Results

Thirty-seven patients were included. There were no differences among the patients in the baseline characteristics and phagocytic activity. At the 6th month, a higher proportion of patients with phagocytic restoration was observed in the BCAA granule group than in the placebo group (68% vs. 5.6%, p < 0.001). The mean phagocytic activity was 75.4% and 63.4% in the BCAA granule and placebo groups, respectively (p < 0.001). Progressive accretion of phagocytic activity was observed during the 3rd and 6th months. There was no difference in hospitalization due to infection (3 vs. 2 events, p = 0.487).

Conclusion

BCAA granules significantly restore phagocytic activity across various stages of cirrhosis. A longer follow-up period is required to demonstrate infection prevention.
Trial registration: www.clinicaltrials.in.th (TCTR20190830005)
Health oil preparation from gardenia seeds by aqueous enzymatic extraction combined with puffing pre-treatment and its properties analysis
Authors: Jin Chengyu , Wang Lingyun , Liu Xiaoying , Lu Yuanchao , Yu Ningxiang , Ye Qin , Nie Xiaohua , Meng Xianghe 
Published: October 2022

Abstract
Gardenia jasminoides Ellis, a typical representative for “homology of medicine and food”, can be used to produce gardenia yellow and edible oil. Here, aqueous enzymatic extraction (AEE) combined with puffing pre-treatment was explored to prepare oil from gardenia seeds. Free oil yield (FOY) by AEE was evaluated in term of selection of enzyme, enzyme combination, as well as puffing ways and conditions. Results suggested that seeds taken as raw materials were better than whole fruit, sequential hydrolysis was superior to single enzymatic hydrolysis. Both wet-heating puffing at 90℃ and dry-heating puffing at 1.0MPa facilitated the release of free oil by AEE, while FOY produced the highest oil yields of 21.8% and 23.2% within 3h, and 58.3%, 68.4% higher than that of un-puffed group, active crocin and geniposide were also completely released. The FOY obtained in this study was far higher than pressing method (10.44%), and close to solvent extraction (25.45%). Also, microstructure analysis indicated that gardenia seeds expanded by dry-heating puffing (1.0MPa) had larger and rougher surface and porous structure than other groups. Moreover, there was no significant difference between oils prepared by various methods in term of nutritional and safety characteristics. AEE coupled with puffing pre-treatment developed is an eco-friendly extraction technology with high efficiency that can be employed to oil preparation enriched with active crocin and geniposide from gardenia seeds.
Cost analysis of hospital-at-home services vs. in-patient medical wards
Authors: Frvd Natalia , Weinstein Orly , shiber shachaf 
Published: October 2022

Abstract
Objective: Hospital-at-home schemes have been widely adopted as an alternative to in-hospital treatment for acutely ill patients. However, their impact on hospital costs remains unclear.
Study setting: we calculated the costs during the index admission and 30 days later of 131 randomly selected acutely ill patients attending a tertiary medical center in 2011-2021 who met the inclusion criteria for hospital-at-home care. study design: findings were compared to a simulation of the same cohort based on assumptions of potential costs and outcomes under a hospital-at-home program.
principal findings: hospital-at-home care was found to incur higher costs during the index admission (+30%) and 30 days after (+14%). It remained costlier on most subpopulation and sensitivity analyses, except when patients were readmitted within 30 days of the index admission (-27%), owing to 30% lower hospital-at-home labor costs, and if the predicted index admission days were decreased by 10% in the hospital-at-home scheme (-11%).
Conclusions: under the assumptions made, there may not be a clear and robust cost reduction to hospitals by the mere application of a hospital-at-home scheme.
Development and application of hepatocellular carcinoma risk prediction model based on clinical characteristics and liver related indexes
Authors: Liu Zhijie , Xu Yue , Wang Wenxuan , Guo Bin , Guo Xiaolan , Zhang Guoyuan , Luo Guangcheng , Wang Qiang 
Published: October 2022

Abstract
Background and aims

Hepatocellular carcinoma (HCC) is difficult to diagnose and has a low survival rate. The survival of patients with HCC is closely related to the stage of diagnosis. Using logistic regression model, this study aimed to identify risk factors associated with HCC and establish a risk prediction model based on clinical characteristics and liver-related indicators to predict the presence of HCC.

Methods

The clinical data of patients in Affiliate Hospital of North Sichuan Medical College from 2016 to 2020 were collected. Based on the time of admission, the cases were divided into training cohort (n = 1739) and validation cohort (n = 467). Using HCC as a dependent variable, the research indicators were incorporated into logistic univariate and multivariate analysis. An HCC risk prediction model, which was called NSMC-HCC model, was then established in training cohort and verified in validation cohort.

Results

The area under receiver operating characteristic curve (AUC) of NSMC-HCC model in HCC diagnosis was 0.960, with sensitivity 94.40% and specificity 95.35% in training cohort, and AUC was 0.966, with sensitivity 90.00% and specificity 94.20% in validation cohort. In early-stage HCC diagnosis, the AUC of NSMC-HCC model was 0.946, with sensitivity 85.93% and specificity 93.62% in training cohort, and AUC was 0.947, with sensitivity 89.10% and specificity 98.49% in validation cohort.

Conclusions

NSMC-HCC model was an effective risk prediction model in HCC and early-stage HCC diagnosis.
Phosphorylation disrupts long-distance electron transport in cytochrome c
Authors: Gomila Alexandre , Pérez-Mejías Gonzalo , Hill Alba Nin , Guerra Alejandra , Casas-Ferrer Laura , Ortiz-Tescari Sthefany , Díaz-Quintana Antonio , Samitier Josep , Rovira Carme , Rosa Miguel A. De la , Díaz-Moreno Irene , Gorostiza Pau , Giannotti Marina , Lagunas Anna 
Published: October 2022

Abstract
It has been recently shown that electron transfer (ET) between mitochondrial cytochrome c (Cc) and the cytochrome c1 subunit of the cytochrome bc1 (Cc1) and Cc can proceed at long-distance through the aqueous solution. Cc is thought to adjust its activity by changing the affinity for its partners via Tyr48 phosphorylation, but it is unknown how it impacts the nanoscopic environment, interaction forces, and long-range ET. Here, we constrain the orientation and separation between Cc1 and Cc or the phosphomimetic Y48pCMF Cc, and deploy an array of single-molecule, bulk, and computational methods to investigate the molecular mechanism of ET regulation by Cc phosphorylation.. We demonstrate that phosphorylation impairs long-range ET, shortens the long-distance charge conduit between the partners, strengthens their interaction, and departs it from equilibrium. These results unveil a nanoscopic view of the interaction between redox protein partners in electron transport chains and its mechanisms of regulation.
Age and Sex Differences in the Eμ-TCL1 adoptive transfer mouse model of CLL
Authors: Sharpe Chia , Perry Elizabeth , Muthusamy Natarajan , Hertlein Erin , Byrd John C. 
Published: October 2022

Abstract
Background:The Eμ-TCL1 syngeneic model is the most widely used mouse model of chronic lymphocytic leukemia and has been extensively used to understand the pathogenesis of select genes, the effect of the immune microenvironment and for pre-clinical drug development studies. Recently there has been an increasing awareness of the impact of age and sex differences on not only the development of cancers but also the efficacy and toxicity of specific cancer therapies. However, despite the predominance of older males in CLL patient demographics, the Eμ-TCL1 adoptive transfer studies have used almost exclusively young female recipient mice.
Methods:In this study we performed primary and secondary adoptive transfer experiments in order to understand the impact of recipient age and sex on the development of disease as assessed by flow cytometry and survival in the Eμ-TCL1 adaptive transfer model.
Results:We found that young female recipients had pro-longed survival in a primary adoptive transfer, however sex differences were not observed in a subsequent secondary adoptive transfer experiment. Furthermore, while recipient age did not have a significant effect on the rate of disease establishment or survival in female mice, aged male mice had a significantly decreased rate of Eμ-TCL1 tumor engraftment.
Conclusions:These findings suggest that age and sex differences must be considered in the experimental design of studies using the Eμ-TCL1 adaptive transfer model of CLL.