bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches
Authors: Taques Barbara , Gamba Humberto , Menegaz Danusa , Silva Fátima , Suzuki Daniela 
Published: June 2022

Abstract
Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses. The responses of Sertoli cells in culture to hormone stimulation were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools. A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na+/K + pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells collected from intact seminiferous tubules and of Sertoli cells in culture. The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na+/K + pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells. Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of non-excitable cells to hormonal stimuli.
Age-varying susceptibility to the Omicron variant (B.1.1.529) of SARS-CoV-2
Authors: Chun June Young , Kim Yongdai , Jeong Hwichang 
Published: June 2022

Abstract
The Omicron variant (B.1.1.529) is estimated to be more transmissible than previous strains of SARS-CoV-2 especially among children, potentially resulting in croup which is characteristic disease in children. However, there have been no reports confirming the relative susceptibility of different age groups to SARS-CoV-2. In this study, we estimated the age-specific susceptibility during the 5th (Omicron driven), 4th (Delta driven), and 3rd (pre-Delta) waves in South Korea, using the age-structured compartmental model. Even after adjusting for contact pattern, vaccination status, and waning of vaccine effectiveness, the susceptibility among age group 0–19 years was approximately 5 times higher during the 5th wave (Omicron driven) than that during the 3rd wave (pre-Delta), and 3 times higher than that during the 4th wave (Delta driven). Here, we found that children are indeed more susceptible to the Omicron variant compared with the previous strains of SARS-CoV-2.
Fusion Classification of Stroke Patients' Biosignals by Weighted Cross-Validation-based Feature Selection (W-CVFS) Method
Authors: Pan Xuejiao , Chen Xiaojuan , Ji Tiecheng , Yu Suli , Sun Yue 
Published: June 2022

Abstract
A multi-source information fusion-based disease class classification of stroke patients was implemented to address the low classification accuracy of pure input motion and electromyographic signals. sEMG sensor MYO arm ring and wearable wireless motion sensor Shimmer were used as data acquisition devices. The Butterworth high-pass filter filtering and envelope thresholding method detected the activity segment. Detection and FIR filtering using the window function method remove interference from the motion signal. A weighted cross-validation-based feature selection (W-CVFS) method is proposed for feature fusion selection. The top 10 features selected by the W-CVFS method and all 18 features are input to the deep neural network for training and testing, and the feature classification result of the W-CVFS method is 79.17%, which is better than the existing mRMR method (66.67%) and ILFS method (62.50%). The classification accuracy of multi-source information fusion was 95.385%, which was higher than that of a single input motion signal or sEMG. The experiments showed that the proposed method can retain the features that have more influence on the classification results and can improve the classification accuracy of the rehabilitation model for stroke patients.
L1-boundedness of rough Fourier integral operators.
Authors: Sindayigaya Joachim 
Published: June 2022

Abstract
In this paper, we study the L1-boundedness of Fourier integral operator T_{\phi,a} with rough symbol a\in L^{\infty}S^{m}_{\rho} and a new class of rough phase \phi. In this class, we extend the L^{\infty}\phi^{2} and non-degeneracy conditions to some generalized derivative estimation and some measure condition respectively. Our main result substantially extends and emproves some known results about L1-boundedness of Fourier integral operator. Moreover, the result in this paper can be used to prove the boundedness of the maximal wave operator.
Petrochemical attributes of glazed architectural elements from Middle- Elamite to Achaemenid excavation sites in Iran
Authors: Raith Michael M. , Abdali Negar , Yule Paul A. 
Published: June 2022

Abstract
Glazed decoration in Iran from the Middle Elamite to the Achaemenid periods includes world art milestones. With the exception of Hasanlu IVB, for most sites comprehensive chemical and mineralogical data are lacking, owing to the generally profound alteration of the vitreous material. To bridge the information gap, and to enable to reconstruct operation production chains in a diachronic fashion, thirty-six glazed artefacts from Qalaichi, Rabat, Hasanlu, Ziwiye, Chogha Zanbil, Susa and Persepolis are studied. The microstructural make-up and alteration phenomena of glazes are characterized by petrographic microscopy, back scattered electron (BSE) imaging, and electron probe microanalysis (WDS-EPMA) on high-quality polished thin sections. Pristine glaze domains at all sites show plant ash soda-lime glass compositions and indicate the use of regionally specific halophyte species for soda production. A distinct feature in the composition of white and turquoise glazes from Qalaichi, Ziwiye and Achaemenid Susa is the employment of sodium-antimonate as white colourant and opacifier. Inter-diffusion and dissolution-precipitation are identified as regionally and temporally contrasting alteration processes.
A creep prediction model for concrete made from pit sand with low silica content
Authors: Pasalli Daud Andang , Tudjono Sri , Nurhuda Ilham 
Published: June 2022

Abstract
Pit sand generally has a lower silica content than sand sourced from rivers or crushed stones. The effect of this sand on the creep of concrete has not yet been fully studied. Understanding the creep on concrete can help to estimate the behaviour of structures in the future. This study aims to investigate the effect of the properties of pit sand with different chemical compositions and grain size distributions on the creep of concrete, and to develop a creep prediction model for concrete made of pit sand with low silica content. This investigation employs laboratory testing to obtain the physical and chemical properties of the sand, as well as its relationship to shrinkage and creep of concrete. The results indicate that shrinkage in concrete is influenced by the water absorption of sand. It is also found that sand with a large Calcium Oxide (CaO) compound has a smaller shrinkage compared to those with small CaO content. This study shows that sand with a high percentage of fine grains and mud content exhibit a higher creep. A concrete creep prediction model is proposed based on the modification of the B3 Model. Modifications are made by proposing a material constant obtained from short creep tests. The creep parameter obtained from the test can be better used to predict the creep of concrete.
Serine/threonine kinase TBK1 promotes Intrahepatic cholangiocarcinoma progression via direct regulation of β-catenin
Authors: Gao Chongqing , Chu Zhenzhen , Xiao Yang , Zhou Xingyan , Wu Junru , Yuan Hui , Jiang Yuchuan , Chen Dong , Zhang Jichun , Yao Nan , Chen Kaiyun , Hong Jian 
Published: June 2022

Abstract

Intrahepatic cholangiocarcinoma (ICC) is an aggressive liver bile duct malignancy with limited therapeutic options. Metastasis is one of the main contributors to ICC progression and poor prognosis. However, the underlying mechanism of ICC metastasis remains largely unknown. Here, we showed that TANK-binding kinase 1 (TBK1), a serine/threonine-protein kinase, was significantly upregulated in tumor tissues of ICC patients with larger tumor diameter, lymph node metastasis, and advanced TNM stage. Consistently, during the different stages of cholangiocarcinoma (CCA) carcinogenesis (hyperplasia, dysplasia, and CCA), TBK1 showed a dynamic increase in spontaneous rat and mouse models. Functional studies showed that enforced expression of TBK1 promoted metastasis both in vitro and in vivo. Mechanistically, TBK1 directly interacts with β-catenin and stimulates its nuclear translocation, further activating the β-catenin-mediated epithelial-mesenchymal transition (EMT) process. Moreover, we demonstrate that the S172 site of TBK1 kinase domain was essential for the interaction between TBK1 and β-catenin as well as for TBK1 mediated β-catenin activation. In addition, high levels of TBK1 in clinical ICC tissues were correlated with elevated nuclear β-catenin levels and predicted worse overall and disease-free survival. A TBK1 inhibitor GSK-8612 and the liver-specific accumulation of DNA/RNA heteroduplex oligonucleotide (TBK1-HDO) significantly reduced TBK1 expression of ICC and inhibited its intrahepatic metastasis. In summary, our study demonstrated that TBK1 could activate β-catenin via protein-protein interaction, then promote EMT and ICC metastasis, which might serve as a potential therapeutic target for patients with cholangiocarcinoma.
Multi-market demand response from pump-controlled open canal systems: an economic MPC approach to pump-scheduling
Authors: van der Heijden Ties , Lugt Dorien , van Nooijen Ronald , Palensky Peter , Abraham Edo 
Published: June 2022

Abstract
Participation in demand response (DR) has been explored for many large energy-using assets based on day ahead electricity markets. In this manuscript, we propose the use of multiple electricity spot markets to enable price-based DR for open canal systems in the Netherlands, where many large pumping stations are used for flood mitigation and control of groundwater levels. In the new strategy for pump-scheduling, we combine the day ahead and intraday electricity markets to be used in a hierarchical receding horizon economic Model Predictive Control (MPC). We formulate the decision problem as a Mixed-Integer Quadratic Problem (MIQP), to be solved to near global optimality. A cost-potential analysis was performed for multiple market strategies and the automatic Frequency Restoration Reserves (aFRRs), using actual market and water system data. We show new insights into the trade-off between CO2 emissions and operating cost, the difference between the German and Dutch markets, and temporal changes in market conditions due to renewable energy integration. We observe that the German energy market is rewarding DR more than the Dutch equivalent, due to the higher renewable energy market penetration. The proposed multi-market strategy leads to a cost decrease of 10 and 16% in the Netherlands in 2017 and 2019, respectively. When applying German market scenarios, we found a cost-saving potential of 56 and 50% in 2017 and 2019, respectively. The cost-saving potential for the aFRR market was found to be up to 12% in the Netherlands and 28% in Germany, through a conservative analysis. The results suggest that the proposed control system, optimising costs over the day ahead, intraday and possibly the aFRR markets, is profitable compared to the current strategy in both the current and future electricity market.
Beyond scarcity and its management: Sociocultural dimensions of the water crisis in the Atacama Desert
Authors: Cantillana Raphael , Iniesta-Arandia Irene 
Published: June 2022

Abstract
The depletion of freshwater sources by indiscriminate industrial actions is one of the direct causes of the current water crisis. One of the emblematic cases of this problem is the Atacama Desert, a unique hyper-arid zone in which an extensive copper industry depletes its scarce freshwater reserves. This territory, occupied for millennia by indigenous groups, is experiencing a water crisis that threatens its very subsistence. This paper uses the ethnographic method and the Water Justice framework to approach the everyday and often invisible issues of this crisis within the Mamiña community. This Quechua community holds an ambivalent position in front of the copper mine project because of its control of the water rights, the economic interests of some of their members, and the role of the Aymara minority. We suggest that researchers in this field pay more attention to the communities’ internal diversity and its relations with the copper industry to understand how the water crisis is detached from its biophysical conceptions to be socioculturally constructed.
Preparation and photocatalytic performance of UIO-66/La-MOF composite
Authors: Tong Haixia , Ji Yu , He Tenghui , He Ruidong , Chen Maolong , Zeng Julan , Wu Daoxin 
Published: June 2022

Abstract
To improve the photocatalytic degradation efficiency of photocatalytic materials UIO-66 and La-MOFs under visible-light irradiation, a series of photocatalytic materials with La and Zr as metal centers and terephthalic acid (H2BDC) and 2-amino terephthalic acid (H2ATA) as organic ligands were prepared by solvothermal method. The photocatalytic materials were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible (UV-vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and Mott-Schottky test. The photocatalytic degradation performance to Rhodamine B of the catalysts was fully investigated. Results show that the H2ATA series had stronger visible-light absorption capacity and better photocatalytic performance. The 0.35 La/Zr-H2ATA composite showed the best photocatalytic degradation. The quenching experiments confirmed that the active species in the photocatalytic degradation were the holes and superoxide radicals. The possible mechanisms of the carrier migration paths in the energy level matching for La/Zr-H2BDC and La/Zr-H2ATA were also discussed in detail.
Information Support of Statistic Research of Budget Investments’ Impact on Socio-Ecological and Economic Development of the Russian Federation regions
Authors: Kovalenko N. N. 
Published: June 2022

Purpose of the study is to estimate the information support on budget investments and analyze the sources of their formation from the standpoint of completeness and reliability of information for their use as an information base for a statistical study of the impact of budget investments on the social, environmental and economic development of the regions of the Russian Federation. The main directions for assessing the information security of the research are: studying the requirements and basic standards for information; specifics and structures of formed information flows; analysis of information resources, including their parameters and characteristics describing their properties and purpose; determination of units of main information elements, their grouping by formation sources, by main users, by formation system.Materials and methods. The research used the theoretical methods: analysis, synthesis, grouping of sources of information on budget investments.Results. The conducted research allowed making a conclusion on a high degree of information security of a rather extensive, multidimensional set of information sources and resources on budget investments. This paper is the basis for the formation of a categorical instrument of statistical research on budget investments. The information array of data on budget investments is aimed at analytical procedures that can be carried out by types, sorts, forms of implementation, budget levels and budget investments realization stages including regional sectioning.Conclusion. The conducted research has a high degree of practical value due to its aim at direction on elaboration of tools for state management of budget investments. Rapid development of forms and resource chances of the formed information flows on budget investments determined by realization of requirements to the publication of data by state bodies and local self-government bodies which have become more often placed on the Internet as data arrays in the format ensuring their automatic processing to be reused in terms of free use.
Establishment of a High Throughput Screening System for GABAA1
Modulators in Living Cells
Authors: Wang Chen , Li Liqin , Zhang Yi , Shi Tong , Chen Xuejun , Zhang Ruihua , Shi Jingjing , Jin Qian , Xu Jianfu 
Published: June 2022

Background:
The incidence of sleep disorders is more than 27% in the worldwide, and
the development of novel sleep drugs that target GABAA receptors is of great interest. Traditional
drug screening methods restrict the discovery of lead compounds, the high-throughput screening
system is a powerful means for the lead compounds discovery of sleep drug.

Methods:
The GABAA1-CHO cell line stably expressing α1β2γ2L was constituted by cotransfection
of α1, β2 and γ2L subunits into CHO-T-Rex cells. The high-throughput screening
method of membrane potential targeting GABAAR was established and optimized. The optimized
method was used to screen the compound library, and the compounds with high activity were obtained.
The active compounds were confirmed in vitro by electrophysiological detection technique, and the
sleep effects of compounds in vivo were detected by pentobarbital sodium sleep model in mice.

Results:
A stable cell line expressing human GABAA1 receptor in CHO-T-Rex cells was generated
and used to establish a functional high-throughput screening assay based on the measurement of
membrane potential changes in living cells by fluorometric imaging plate reader (FLIPR). The assay
was further used to detect the dose-effect relationships of tool compounds, the EC50 values of
agonist GABA (137.42 ± 26.31 nM), positive allosteric modulator diazepam (3.22 ± 0.73 μM), and
antagonist gabazine (0.16 ± 0.04 μM), blocking agents bicuculine (0.47 ± 0.06 μM) and PTX (6.39
± 1.17 μM). In the meanwhile, the compounds were screened from a compound library (10000) by
the membrane potential dye assay. Selected 4 active compounds were further identified for their
EC50 values in vitro by electrophysiological method, the EC50 values of 4 compounds were further
determined as 1.37 ± 0.43 μM, 0.69 ± 0.17 μM, 0.77 ± 0.16 μM, and 1.62 ± 0.29 μM. Furthermore,
the pentobarbital sleep rate and the sleep time of mice pretreated with 4 active compounds by oral
administration were significantly increased compared with mice pretreated with a negative control
in vivo experiment.

Conclusion:
We successfully generated a stable CHO cell line expressing human GABAA1 by induced
expression strategy which decreased cytotoxicity. Then, developed an efficient membrane
potential detection method for high-throughput screening, the assay based on the stable cell line
could distinguish different types of GABAA1 modulators, which would be an effective in vitro system
to screen the GABAAR-targeted compounds. Compared with the patch clamp electrophysiological
detection method, the membrane potential detection method has higher detection flux for
compounds and higher detection sensitivity for active compounds.
Vitamin D Metabolism Gene Polymorphisms and Their Associated Disorders: A
Literature Review
Authors: Abouzid Mohamed , Główka Franciszek , Kagan Leonid , Karaźniewicz-Łada Marta 
Published: June 2022

Background:
Vitamin D is a fat-soluble vitamin, and it is a potential key factor to maintain a healthy status. Various observational studies have reported the association between vitamin D deficiency and an elevated risk of osteoporosis, cardiovascular disease, diabetes mellitus, and certain types of cancers. The number of studies that investigated the genetic determinants of vitamin D hydroxy metabolism has been growing, still, its association with the genetic variants remains unclear, particularly those genes related to vitamin D metabolism.

Aim:
This work is a comprehensive review of available evidence of the effect of genetic variants on vitamin D metabolism and their impact on vitamin D status in the human body, disorders including coronavirus disease 2019 infection, and its importance for clinical investigators and public health.

Results:
Genome-wide association studies and candidate gene studies show that circulating levels of vitamin D are being influenced by genetic factors. These genetic changes are implicated in various pathways of vitamin D, such as metabolism and transport. It is also involved in the formation of the ternary complex (vitamin D receptor - retinoid receptor - transcription factor II B).

Conclusion:
Linkage studies may fail to identify replicated genetic architecture of vitD metabolism, Genome-wide association studies and the candidate gene approach have shown reproducible influences of gene control on vitD status.
The Combination of Baicalin with Knockdown of mir148a Gene
Suppresses Cell Viability and Proliferation and Induces the Apoptosis and
Autophagy of Human Glioblastoma Multiforme T98G and U87MG Cells
Authors: Paul-Samojedny Monika , Liduk Emilia , Kowalczyk Małgorzata , Borkowska Paulina , Zielińska Aleksandra , Suchanek-Raif Renata , Kowalski Jan 
Published: June 2022

Background:
Glioblastoma multiforme (GBM) is a heterogeneous and highly vascularized
brain tumor that avoids apoptosis due to P-glycoprotein (P-gp) mediated multidrug resistance.
Therefore, the development of new therapeutic strategies that induce apoptosis and inhibit proliferation
is urgently warranted.

Objectives:
We examined the efficacy of the combination of baicalin (BAI) and knockdown of
miR-148a gene in human glioblastoma T98G and U87MG cell lines.

Methods:
T98G and U87MG cells were transfected with miR148a siRNA. The influence of miR-
148a siRNA in combination with BAI on T98G and U87MG cell viability, proliferation, apoptosis,
and autophagy was evaluated as well. Alterations in the mRNA expression of autophagy-related
genes were analyzed using RT-qPCR.

Results:
The transfection of T98G and U87MG cells with miR148a specific siRNA and exposition
on baicalin led to a significant reduction in cell viability and proliferation, the accumulation of sub
G1-phase cells and a reduced population of cells in the S and G2/M phases (only in U87MG cell
line), increased population of cells in the S phase in T98G cell line and apoptosis or necrosis induction
and induction of autophagy for both cell lines.

Conclusion:
The siRNA-induced miR-148a mRNA knockdown in combination with baicalin may
offer a novel therapeutic strategy to more effectively control the growth of human GBM cells.
Thus, knockdown of this gene in combination with baicalin inhibits proliferation (cell cycle arrest
in the S phase in T98G but not in U87MG cells), induces apoptosis, and regulates autophagy in
T98G and U87MG cells. However, further studies are urgently needed to confirm a positive phenomenon
for the treatment of GBM.