bluuebunny/crossref_metadata_2025_split huggingface dataset:
The antileukemic activity of decitabine upon PML/RARA-negative AML blasts is supported by all-trans retinoic acid: in vitro and in vivo evidence for cooperation
Authors: Meier Ruth , Greve Gabriele , Zimmer Dennis , Bresser Helena , Berberich Bettina , Langova Ralitsa , Stomper Julia , Rubarth Anne , Feuerbach Lars , Lipka Daniel B. , Hey Joschka , Grüning Björn , Brors Benedikt , Duyster Justus , Plass Christoph , Becker Heiko , Lübbert Michael 
Published: August 2022

AbstractThe prognosis of AML patients with adverse genetics, such as a complex, monosomal karyotype and TP53 lesions, is still dismal even with standard chemotherapy. DNA-hypomethylating agent monotherapy induces an encouraging response rate in these patients. When combined with decitabine (DAC), all-trans retinoic acid (ATRA) resulted in an improved response rate and longer overall survival in a randomized phase II trial (DECIDER; NCT00867672). The molecular mechanisms governing this in vivo synergism are unclear. We now demonstrate cooperative antileukemic effects of DAC and ATRA on AML cell lines U937 and MOLM-13. By RNA-sequencing, derepression of >1200 commonly regulated transcripts following the dual treatment was observed. Overall chromatin accessibility (interrogated by ATAC-seq) and, in particular, at motifs of retinoic acid response elements were affected by both single-agent DAC and ATRA, and enhanced by the dual treatment. Cooperativity regarding transcriptional induction and chromatin remodeling was demonstrated by interrogating the HIC1, CYP26A1, GBP4, and LYZ genes, in vivo gene derepression by expression studies on peripheral blood blasts from AML patients receiving DAC + ATRA. The two drugs also cooperated in derepression of transposable elements, more effectively in U937 (mutated TP53) than MOLM-13 (intact TP53), resulting in a “viral mimicry” response. In conclusion, we demonstrate that in vitro and in vivo, the antileukemic and gene-derepressive epigenetic activity of DAC is enhanced by ATRA.
SOCS2-enhanced ubiquitination of SLC7A11 promotes ferroptosis and radiosensitization in hepatocellular carcinoma
Authors: Chen Qianping , Zheng Wang , Guan Jian , Liu Hongxia , Dan Yao , Zhu Lin , Song Yimeng , Zhou Yuchuan , Zhao Xinrui , Zhang Yuhong , Bai Yang , Pan Yan , Zhang Jianghong , Shao Chunlin 
Published: August 2022

AbstractRadioresistance is a principal culprit for the failure of radiotherapy in hepatocellular carcinoma (HCC). Insights on the regulation genes of radioresistance and underlying mechanisms in HCC are awaiting for profound investigation. In this study, the suppressor of cytokine signaling 2 (SOCS2) were screened out by RNA-seq and bioinformatics analyses as a potential prognosis predictor of HCC radiotherapy and then were determined to promote radiosensitivity in HCC both in vivo or in vitro. Meanwhile, the measurements of ferroptosis negative regulatory proteins of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), intracellular lipid peroxidation and Fe2+ concentration suggested that a high level of ferroptosis contributed to the radiosensitization of HCC. Moreover, SOCS2 and SLC7A11 were expressed oppositely in HCC clinical tissues and tumour xenografts with different radiosensitivities. Mechanistically, the N-terminal domain of SLC7A11 was specifically recognized by the SH2-structural domain of SOCS2. While the L162 and C166 of SOCS2-BOX region could bind elongin B/C compound to co-form a SOCS2/elongin B/C complex to recruit ubiquitin molecules. Herein, SOCS2 served as a bridge to transfer the attached ubiquitin to SLC7A11 and promoted K48-linked polyubiquitination degradation of SLC7A11, which ultimately led to the onset of ferroptosis and radiosensitization of HCC. In conclusion, it was demonstrated for the first time that high-expressed SOCS2 was one of the biomarkers predicting radiosensitivity of HCC by advancing the ubiquitination degradation of SLC7A11 and promoting ferroptosis, which indicates that targeting SOCS2 may enhance the efficiency of HCC radiotherapy and improve the prognosis of patients.
Ultra-strong spin–orbit coupling and topological moiré engineering in twisted ZrS2 bilayers
Authors: Claassen Martin , Xian Lede , Kennes Dante M. , Rubio Angel 
Published: August 2022

AbstractWe predict that twisted bilayers of 1T-ZrS2 realize a novel and tunable platform to engineer two-dimensional topological quantum phases dominated by strong spin-orbit interactions. At small twist angles, ZrS2 heterostructures give rise to an emergent and twist-controlled moiré Kagome lattice, combining geometric frustration and strong spin-orbit coupling to give rise to a moiré quantum spin Hall insulator with highly controllable and nearly-dispersionless bands. We devise a generic pseudo-spin theory for group-IV transition metal dichalcogenides that relies on the two-component character of the valence band maximum of the 1T structure at Γ, and study the emergence of a robust quantum anomalous Hall phase as well as possible fractional Chern insulating states from strong Coulomb repulsion at fractional fillings of the topological moiré Kagome bands. Our results establish group-IV transition metal dichalcogenide bilayers as a novel moiré platform to realize strongly-correlated topological phases in a twist-tunable setting.
Comparison of the 2021 COVID-19 roadmap projections against public health data in England
Authors: Keeling Matt J. , Dyson Louise , Tildesley Michael J. , Hill Edward M. , Moore Samuel 
Published: August 2022

AbstractControl and mitigation of the COVID-19 pandemic in England has relied on a combination of vaccination and non-pharmaceutical interventions (NPIs). Some of these NPIs are extremely costly (economically and socially), so it was important to relax these promptly without overwhelming already burdened health services. The eventual policy was a Roadmap of four relaxation steps throughout 2021, taking England from lock-down to the cessation of all restrictions on social interaction. In a series of six Roadmap documents generated throughout 2021, models assessed the potential risk of each relaxation step. Here we show that the model projections generated a reliable estimation of medium-term hospital admission trends, with the data points up to September 2021 generally lying within our 95% prediction intervals. The greatest uncertainties in the modelled scenarios came from vaccine efficacy estimates against novel variants, and from assumptions about human behaviour in the face of changing restrictions and risk.
Double-Bilayer polar nanoregions and Mn antisites in (Ca, Sr)3Mn2O7
Authors: Miao Leixin , Hasin Kishwar-E , Moradifar Parivash , Mukherjee Debangshu , Wang Ke , Cheong Sang-Wook , Nowadnick Elizabeth A. , Alem Nasim 
Published: August 2022

AbstractThe layered perovskite Ca3Mn2O7(CMO) is a hybrid improper ferroelectric candidate proposed for room temperature multiferroicity, which also displays negative thermal expansion behavior due to a competition between coexisting polar and nonpolar phases. However, little is known about the atomic-scale structure of the polar/nonpolar phase coexistence or the underlying physics of its formation and transition. In this work, we report the direct observation of double bilayer polar nanoregions (db-PNRs) in Ca2.9Sr0.1Mn2O7using aberration-corrected scanning transmission electron microscopy (S/TEM). In-situ TEM heating experiments show that the db-PNRs can exist up to 650 °C. Electron energy loss spectroscopy (EELS) studies coupled with first-principles calculations demonstrate that the stabilization mechanism of the db-PNRs is directly related to an Mn oxidation state change (from 4+ to 2+), which is linked to the presence of Mn antisite defects. These findings open the door to manipulating phase coexistence and achieving exotic properties in hybrid improper ferroelectric.
Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering
Authors: Ling Chen , Peabody George L. , Salvachúa Davinia , Kim Young-Mo , Kneucker Colin M. , Calvey Christopher H. , Monninger Michela A. , Munoz Nathalie Munoz , Poirier Brenton C. , Ramirez Kelsey J. , St. John Peter C. , Woodworth Sean P. , Magnuson Jon K. , Burnum-Johnson Kristin E. , Guss Adam M. , Johnson Christopher W. , Beckham Gregg T. 
Published: August 2022

AbstractMuconic acid is a bioprivileged molecule that can be converted into direct replacement chemicals for incumbent petrochemicals and performance-advantaged bioproducts. In this study, Pseudomonas putida KT2440 is engineered to convert glucose and xylose, the primary carbohydrates in lignocellulosic hydrolysates, to muconic acid using a model-guided strategy to maximize the theoretical yield. Using adaptive laboratory evolution (ALE) and metabolic engineering in a strain engineered to express the D-xylose isomerase pathway, we demonstrate that mutations in the heterologous D-xylose:H+ symporter (XylE), increased expression of a major facilitator superfamily transporter (PP_2569), and overexpression of aroB encoding the native 3-dehydroquinate synthase, enable efficient muconic acid production from glucose and xylose simultaneously. Using the rationally engineered strain, we produce 33.7 g L−1 muconate at 0.18 g L−1 h−1 and a 46% molar yield (92% of the maximum theoretical yield). This engineering strategy is promising for the production of other shikimate pathway-derived compounds from lignocellulosic sugars.
Global systematic review with meta-analysis reveals yield advantage of legume-based rotations and its drivers
Authors: Zhao Jie , Chen Ji , Beillouin Damien , Lambers Hans , Yang Yadong , Smith Pete , Zeng Zhaohai , Olesen Jørgen E. , Zang Huadong 
Published: August 2022

AbstractDiversified cropping systems, especially those including legumes, have been proposed to enhance food production with reduced inputs and environmental impacts. However, the impact of legume pre-crops on main crop yield and its drivers has never been systematically investigated in a global context. Here, we synthesize 11,768 yield observations from 462 field experiments comparing legume-based and non-legume cropping systems and show that legumes enhanced main crop yield by 20%. These yield advantages decline with increasing N fertilizer rates and crop diversity of the main cropping system. The yield benefits are consistent among main crops (e.g., rice, wheat, maize) and evident across pedo-climatic regions. Moreover, greater yield advantages (32% vs. 7%) are observed in low- vs. high-yielding environments, suggesting legumes increase crop production with low inputs (e.g., in Africa or organic agriculture). In conclusion, our study suggests that legume-based rotations offer a critical pathway for enhancing global crop production, especially when integrated into low-input and low-diversity agricultural systems.
Ferritin-mediated iron detoxification promotes hypothermia survival in Caenorhabditis elegans and murine neurons
Authors: Pekec Tina , Lewandowski Jarosław , Komur Alicja A. , Sobańska Daria , Guo Yanwu , Świtońska-Kurkowska Karolina , Małecki Jędrzej M. , Dubey Abhishek Anil , Pokrzywa Wojciech , Frankowski Marcin , Figiel Maciej , Ciosk Rafal 
Published: August 2022

AbstractHow animals rewire cellular programs to survive cold is a fascinating problem with potential biomedical implications, ranging from emergency medicine to space travel. Studying a hibernation-like response in the free-living nematode Caenorhabditis elegans, we uncovered a regulatory axis that enhances the natural resistance of nematodes to severe cold. This axis involves conserved transcription factors, DAF-16/FoxO and PQM-1, which jointly promote cold survival by upregulating FTN-1, a protein related to mammalian ferritin heavy chain (FTH1). Moreover, we show that inducing expression of FTH1 also promotes cold survival of mammalian neurons, a cell type particularly sensitive to deterioration in hypothermia. Our findings in both animals and cells suggest that FTN-1/FTH1 facilitates cold survival by detoxifying ROS-generating iron species. We finally show that mimicking the effects of FTN-1/FTH1 with drugs protects neurons from cold-induced degeneration, opening a potential avenue to improved treatments of hypothermia.
Phylogenetically and catabolically diverse diazotrophs reside in deep-sea cold seep sediments
Authors: Dong Xiyang , Zhang Chuwen , Peng Yongyi , Zhang Hong-Xi , Shi Ling-Dong , Wei Guangshan , Hubert Casey R. J. , Wang Yong , Greening Chris 
Published: August 2022

AbstractMicrobially mediated nitrogen cycling in carbon-dominated cold seep environments remains poorly understood. So far anaerobic methanotrophic archaea (ANME-2) and their sulfate-reducing bacterial partners (SEEP-SRB1 clade) have been identified as diazotrophs in deep sea cold seep sediments. However, it is unclear whether other microbial groups can perform nitrogen fixation in such ecosystems. To fill this gap, we analyzed 61 metagenomes, 1428 metagenome-assembled genomes, and six metatranscriptomes derived from 11 globally distributed cold seeps. These sediments contain phylogenetically diverse nitrogenase genes corresponding to an expanded diversity of diazotrophic lineages. Diverse catabolic pathways were predicted to provide ATP for nitrogen fixation, suggesting diazotrophy in cold seeps is not necessarily associated with sulfate-dependent anaerobic oxidation of methane. Nitrogen fixation genes among various diazotrophic groups in cold seeps were inferred to be genetically mobile and subject to purifying selection. Our findings extend the capacity for diazotrophy to five candidate phyla (Altarchaeia, Omnitrophota, FCPU426, Caldatribacteriota and UBA6262), and suggest that cold seep diazotrophs might contribute substantially to the global nitrogen balance.
Temperature-dependent dual-mode thermal management device with net zero energy for year-round energy saving
Authors: Zhang Quan , Lv Yiwen , Wang Yufeng , Yu Shixiong , Li Chenxi , Ma Rujun , Chen Yongsheng 
Published: August 2022

AbstractReducing needs for heating and cooling from fossil energy is one of the biggest challenges, which demand accounts for almost half of global energy consumption, consequently resulting in complicated climatic and environmental issues. Herein, we demonstrate a high-performance, intelligently auto-switched and zero-energy dual-mode radiative thermal management device. By perceiving temperature to spontaneously modulate electromagnetic characteristics itself, the device achieves ~859.8 W m−2 of average heating power (∼91% of solar-thermal conversion efficiency) in cold and ~126.0 W m−2 of average cooling power in hot, without any external energy consumption during the whole process. Such a scalable, cost-effective device could realize two-way temperature control around comfortable temperature zone of human living. A practical demonstration shows that the temperature fluctuation is reduced by ~21 K, compared with copper plate. Numerical prediction indicates that this real zero-energy dual-mode thermal management device has a huge potential for year-round energy saving around the world and provides a feasible solution to realize the goal of Net Zero Carbon 2050.
Phenotypic correlates of the working dog microbiome
Authors: Craddock Hillary A. , Godneva Anastasia , Rothschild Daphna , Motro Yair , Grinstein Dan , Lotem-Michaeli Yuval , Narkiss Tamar , Segal Eran , Moran-Gilad Jacob 
Published: August 2022

AbstractDogs have a key role in law enforcement and military work, and research with the goal of improving working dog performance is ongoing. While there have been intriguing studies from lab animal models showing a potential connection between the gut microbiome and behavior or mental health there is a dearth of studies investigating the microbiome-behavior relationship in working dogs. The overall objective of this study was to characterize the microbiota of working dogs and to determine if the composition of the microbiota is associated with behavioral and performance outcomes. Freshly passed stools from each working canine (Total n = 134) were collected and subject to shotgun metagenomic sequencing using Illumina technology. Behavior, performance, and demographic metadata were collected. Descriptive statistics and prediction models of behavioral/phenotypic outcomes using gradient boosting classification based on Xgboost were used to study associations between the microbiome and outcomes. Regarding machine learning methodology, only microbiome features were used for training and predictors were estimated in cross-validation. Microbiome markers were statistically associated with motivation, aggression, cowardice/hesitation, sociability, obedience to one trainer vs many, and body condition score (BCS). When prediction models were developed based on machine learning, moderate predictive power was observed for motivation, sociability, and gastrointestinal issues. Findings from this study suggest potential gut microbiome markers of performance and could potentially advance care for working canines.
Recent advances in biodegradable polymers for sustainable applications
Authors: Samir Aya , Ashour Fatma H. , Hakim A. A. Abdel , Bassyouni Mohamed 
Published: August 2022

AbstractThe interest in producing biodegradable polymers by chemical treatment, microorganisms and enzymes has increased to make it easier to dispose after the end of its use without harming the environment. Biodegradable polymers reported a set of issues on their way to becoming effective materials. In this article, biodegradable polymers, treatment, composites, blending and modeling are studied. Environmental fate and assessment of biodegradable polymers are discussed in detail. The forensic engineering of biodegradable polymers and understanding of the relationships between their structure, properties, and behavior before, during, and after practical applications are investigated.
Community lung health service design for COPD patients in China by the Breathe Well group
Authors: Pang Hui , Pan Zihan , Adams Rachel , Duncan Eleanor , Chi Chunhua , Kong Xia , Adab Peymané , Cheng Kar Keung , Cooper Brendan G. , Correia-de-Sousa Jaime , Dickens Andrew P. , Enocson Alexandra , Farley Amanda , Gale Nicola , Jolly Kate , Jowett Sue , Maglakelidze Mariam , Maghlakelidze Tamaz , Martins Sonia , Sitch Alice , Stavrik Katarina , Stelmach Raphael , Turner Alice , Williams Siân , Jordan Rachel E. 
Published: August 2022

AbstractCOPD is increasingly common in China but is poorly understood by patients, medications are not used as prescribed and there is no access to recommended non-pharmacological treatment. We explored COPD patients’ and general practitioners’ (GPs) knowledge of COPD, views on its management and the acceptability of a flexible lung health service (LHS) offering health education, exercise, self-management, smoking cessation and mental health support. Using a convergent mixed methods design, data were collected from patients and GPs using focus groups (FGs) in four Chinese cities, questionnaires were also used to collect data from patients. FGs were audio-recorded and transcribed. Quantitative data were analysed descriptively, thematic framework analysis was used for the qualitative data. Two-hundred fifty-one patients completed the questionnaire; 39 patients and 30 GPs participated in ten separate FGs. Three overarching themes were identified: patients’ lack of knowledge/understanding of COPD, current management of COPD not meeting patients’ needs and LHS design, which was well received by patients and GPs. Participants wanted COPD education, TaiChi, psychological support and WeChat for social support. 39% of survey responders did not know what to do when their breathing worsened and 24% did not know how to use their inhalers. 36% of survey respondents requested guided relaxation. Overall, participants did not fully understand the implications of COPD and current treatment was sub-optimal. There was support for developing a culturally appropriate intervention meeting Chinese patients’ needs, health beliefs, and local healthcare delivery. Further research should explore the feasibility of such a service.
Ab initio predictions link the neutron skin of 208Pb to nuclear forces
Authors: Hu Baishan , Jiang Weiguang , Miyagi Takayuki , Sun Zhonghao , Ekström Andreas , Forssén Christian , Hagen Gaute , Holt Jason D. , Papenbrock Thomas , Stroberg S. Ragnar , Vernon Ian 
Published: August 2022

AbstractHeavy atomic nuclei have an excess of neutrons over protons, which leads to the formation of a neutron skin whose thickness is sensitive to details of the nuclear force. This links atomic nuclei to properties of neutron stars, thereby relating objects that differ in size by orders of magnitude. The nucleus208Pb is of particular interest because it exhibits a simple structure and is experimentally accessible. However, computing such a heavy nucleus has been out of reach for ab initio theory. By combining advances in quantum many-body methods, statistical tools and emulator technology, we make quantitative predictions for the properties of208Pb starting from nuclear forces that are consistent with symmetries of low-energy quantum chromodynamics. We explore 109different nuclear force parameterizations via history matching, confront them with data in select light nuclei and arrive at an importance-weighted ensemble of interactions. We accurately reproduce bulk properties of208Pb and determine the neutron skin thickness, which is smaller and more precise than a recent extraction from parity-violating electron scattering but in agreement with other experimental probes. This work demonstrates how realistic two- and three-nucleon forces act in a heavy nucleus and allows us to make quantitative predictions across the nuclear landscape.
Dopamine D1-like receptor blockade and stimulation decreases operant responding for nicotine and food in male and female rats
Authors: Chellian Ranjithkumar , Behnood-Rod Azin , Wilson Ryann , Lin Karen , King Grace Wing-Yan , Ruppert-Gomez Marcella , Teter Alexandria Nicole , Febo Marcelo , Bruijnzeel Adriaan W. 
Published: August 2022

AbstractDopamine has been implicated in the reinforcing effects of smoking. However, there remains a need for a better understanding of the effects of dopamine D1-like receptor agonists on nicotine intake and the role of sex differences in the effects of dopaminergic drugs on behavior. This work studied the effects of D1-like receptor stimulation and blockade on operant responding for nicotine and food and locomotor activity in male and female rats. The effects of the D1-like receptor antagonist SCH 23390 (0.003, 0.01, 0.03 mg/kg) and the D1-like receptor agonist A77636 (0.1, 0.3, 1 mg/kg) on responding for nicotine and food, and locomotor activity were investigated. The effects of SCH 23390 were investigated 15 min and 24 h after treatment, and the effects of the long-acting drug A77636 were investigated 15 min, 24 h, and 48 h after treatment. Operant responding for nicotine and food and locomotor activity were decreased immediately after treatment with SCH 23390. Treatment with SCH 23390 did not have any long-term effects. Operant responding for nicotine was still decreased 48 h after treatment with A77636, and food responding was decreased up to 24 h after treatment. Treatment with A77636 only decreased locomotor activity at the 48 h time point. There were no sex differences in the effects of SCH 23390 or A77636. In conclusion, the D1-like receptor antagonist SCH 23390 reduces nicotine intake and causes sedation in rats. Stimulation of D1-like receptors with A77636 decreases nicotine intake at time points that the drug does not cause sedation.