bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-22 and miR-205 Drive Tumor Aggressiveness of Mucoepidermoid Carcinomas of Salivary Glands
Authors: Naakka Erika , Barros-Filho Mateus Camargo , Adnan-Awad Shady , Al-Samadi Ahmed , Marchi Fábio Albuquerque , Kuasne Hellen , Korelin Katja , Suleymanova Ilida , Brown Amy Louise , Scapulatempo-Neto Cristovam , Lourenço Silvia Vanessa , Castilho Rogério Moraes , Kowalski Luiz Paulo , Mäkitie Antti , Araújo Vera Cavalcanti , Leivo Ilmo , Rogatto Silvia Regina , Salo Tuula , Passador-Santos Fabricio 
Published: February 2022

ObjectivesTo integrate mRNA and miRNA expression profiles of mucoepidermoid carcinomas (MECs) and normal salivary gland (NSGs) tissue samples and identify potential drivers.Material and MethodsGene and miRNA expression arrays were performed in 35 MECs and six NSGs.ResultsWe found 46 differentially expressed (DE) miRNAs and 3,162 DE mRNAs. Supervised hierarchical clustering analysis of the DE transcripts revealed two clusters in both miRNA and mRNA profiles, which distinguished MEC from NSG samples. The integrative miRNA-mRNA analysis revealed a network comprising 696 negatively correlated interactions (44 miRNAs and 444 mRNAs) involving cell signaling, cell cycle, and cancer-related pathways. Increased expression levels of miR-205-5p and miR-224-5p and decreased expression levels of miR-139-3p, miR-145-3p, miR-148a-3p, miR-186-5p, miR-338-3p, miR-363-3p, and miR-4324 were significantly related to worse overall survival in MEC patients. Two overexpressed miRNAs in MEC (miR-22 and miR-205) were selected for inhibition by the CRISPR-Cas9 method. Cell viability, migration, and invasion assays were performed using an intermediate grade MEC cell line. Knockout of miR-205 reduced cell viability and enhanced ZEB2 expression, while miR-22 knockout reduced cell migration and invasion and enhanced ESR1 expression. Our results indicate a distinct transcriptomic profile of MEC compared to NSG, and the integrative analysis highlighted miRNA-mRNA interactions involving cancer-related pathways, including PTEN and PI3K/AKT.ConclusionThe in vitro functional studies revealed that miR-22 and miR-205 deficiencies reduced the viability, migration, and invasion of the MEC cells suggesting they are potential oncogenic drivers in MEC.
Prevalence and Risk Factors of Asthma in Preschool Children in Shanghai, China: A Cross-Sectional Study
Authors: Ren Jie , Xu Jing , Zhang Pingbo , Bao Yixiao 
Published: February 2022

BackgroundPrevious studies have shown the increasing prevalence of childhood asthma around the world as well as in China. Nevertheless, little is known about the epidemiology of asthma in preschool children. Thus, the present study investigated the prevalence and severity of asthma in Shanghai, China, and identified related risk factors for asthma in children at the age of 3–6.MethodsInformation was obtained through the International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire. Risk factor analysis was carried out using univariate and multivariate logistic regression. The odds ratio (OR)/adjusted odds ratio (aOR) and the 95% confidence interval (CI) were determined.ResultsA total of 6,183 children (3,165 boys and 3,018 girls) covering 12 communities were included in our study, with an average age of 4.2 ± 0.7 years. The prevalence of ever asthma, current asthma, and physician-diagnosed asthma was 16.0, 11.2, and 5.3%, respectively. Parental allergic history, including rhinitis and asthma, was significantly associated with asthma symptoms. The strongest association with current asthma was paternal asthma (aOR = 5.91, 95% CI 3.87–9.01), and maternal asthma had the second strongest association with current asthma (3.85; 2.40–6.17). Among personal factors, allergic rhinitis history, eczema history, food allergy history, and antibiotic use in the first year of life were significantly associated with current asthma (aOR = 1.89, 95% CI 1.52–2.34; aOR = 1.34, 95% CI 1.09–1.64; aOR = 1.68, 95% CI 1.37–2.06; aOR = 1.53, 95% CI 1.25–1.87, respectively). More than once paracetamol use per year and per month were associated with current asthma in a dose–response manner. Additionally, female sex was an independent protective factor for ever asthma (0.82; 0.70–0.96). Among environmental factors, dampness or mildew at home was an independent risk factor for ever asthma (1.50; 1.15–1.97) and current asthma (1.63; 1.21–2.19). Floor heating system was significantly associated with ever asthma (1.57; 1.25–1.98) and current asthma (1.36; 1.04–1.78). Furthermore, dampness or mildew, infrequent house cleaning, and truck traffic in residential streets were significantly associated with asthma symptoms only in old communities, while paracetamol use in the first year of life and flooring materials were significant factors only in new communities.ConclusionThe prevalence of asthma has increased among preschool children in Shanghai over the past three decades. The identified risk factors indicated the combined effects of genetic, personal, and environmental factors on asthma symptoms. Differentiated strategies should be taken for preventing asthma in old and new communities.
Electrocardiographic Characteristics and Catheter Ablation of Ventricular Arrhythmias Originating From the Moderator Band in Children
Authors: Jiang Diandong , Lv Jianli , Han Bo , Yang Xiaofei , Zhao Lijian , Yi Yingchun , Long Deyong , Sang Caihua 
Published: February 2022

AimsTo investigate the electrocardiographic (ECG) characteristics and catheter ablation of ventricular arrhythmias (VAs) originating from the moderator band (MB) in children.MethodsA total of six children who had VAs originating from the MB—as confirmed by electrophysiological study—and who underwent catheter ablation between January 2016 and December 2020 were retrospectively reviewed. During the procedure, a three-dimensional electroanatomic mapping system was used to facilitate three-dimensional anatomical reconstruction, mapping and ablation. Patients' clinical characteristics, ECG features and procedural data were collected and analyzed.ResultsThe mean age was 8.4 ± 2.6 years (range: 5.3–11 years) and mean weight was 27.7 ± 11.4 kg (range: 17–47 kg). Four patients presented with frequent premature ventricular contraction (PVC), one patient presented with frequent PVC and non-sustained ventricular tachycardia, and one patient presented with sustained monomorphic ventricular tachycardia. The QRS duration averaged 126.3 ± 4.6 ms. In all patients, the VAs had left bundle branch block QRS with left superior frontal plane axes, rapid downstrokes of the QRS in the precordial leads, and late precordial transitions (&gt;V4). During the same period, 10 cases of VAs originated from the posterior-lateral wall of the tricuspid annulus, with a mean QRS duration of 152.8 ± 6.4 ms. Compared to that, VAs of MB origin have narrower QRS widths, downstroke slopes in the inferior lead, sharper downstroke slopes in the precordial lead, and smaller R-wave amplitudes in the V6 lead. All patients experienced immediate ablation success with activations earlier than QRS by 26.0 ± 3.5 ms, and no procedural complications occurring. Only one case had recurrent PVC during a follow-up period ranging from 6 to 36 months.ConclusionMB VAs in children have distinctive ECG morphology and electrophysiological characteristics. Catheter ablation using a three-dimensional electroanatomic mapping system is safe and effective in these patients.
Additional Use of Prostacyclin Analogs in Patients With Pulmonary Arterial Hypertension: A Meta-Analysis
Authors: Wang Pengwei , Deng Jiaxin , Zhang Quanying , Feng Hongyan , Zhang Yongheng , Lu Yizhong , Han Lizhu , Yang Pengfei , Deng Zhijian 
Published: February 2022

Background: Combination therapy has become an attractive option in pulmonary arterial hypertension (PAH) treatment. The aim of this study was to investigate whether additional use of prostacyclin analogs could exert any additional benefits over background targeted therapies in PAH patients.Methods: Searches were performed on PubMed, Embase, and ClinicalTrials.gov from inception to 1 October 2021. Randomized controlled trials were included if patients had been treated with prostacyclin analog-containing combination therapy and compared with the use of other PAH-specific background therapies. The bias risk and statistical analysis of the enrolled studies were performed with RevMan 5.1. Sensitivity analysis and funnel plot were used to evaluate the stability and publication bias, respectively. PROSPERO registered number CRD42021284196.Results: Ten trials involving 1828 patients were included. Prostacyclin analog treatment was associated with greater improvement in clinical worsening (risk ratio [RR], 0.70; 95% confidence interval [CI], 0.57–0.86), 6-min walk distance (mean difference [MD], 37.17 m; 95% CI, 3.01–71.33 m), NYHA/WHO functional class (RR, 1.58; 95% CI, 1.21–2.05), mean pulmonary artery pressure (MD, −9.23 mmHg; 95% CI, −17.44 to −1.03 mmHg), and cardiac index (MD, 0.41 L/min/m2; 95% CI, 0.26–0.55 L/min/m2) than the control group. No significant differences in pulmonary vascular resistance (MD, −137.22 dyn·s/cm5; 95% CI, −272.61 to −1.84 dyn·s/cm5) and all-cause mortality (RR, 0.96; 95% CI, 0.57–1.61) were found between the prostacyclin analog group and control group. Of note, more adverse events (RR, 1.07; 95% CI, 1.02–1.13) occurred in the prostacyclin analog group but no significant increase in serious adverse events (RR, 1.25; 95% CI, 0.75–2.11).Conclusion: Additional prostacyclin analog treatment exerted benefits on clinical worsening, exercise capacity, functional class, mean pulmonary artery pressure, and cardiac index in PAH patients, but it was associated with overall risk of adverse events.Clinical Trial Registration:https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021284196, identifier CRD42021284196.
Rapid Weight Loss Practices in Grapplers Competing in Combat Sports
Authors: Ranisavljev Marijana , Kuzmanovic Jovan , Todorovic Nikola , Roklicer Roberto , Dokmanac Milorad , Baic Mario , Stajer Valdemar , Ostojic Sergej M. , Drid Patrik 
Published: February 2022

BackgroundGrappling is a wrestling style that combines different techniques such as freestyle wrestling, jiu-jitsu, judo, sambo, and others. As with other combat sports, it requires categorizing the athletes in weight classes, which leads to the use of certain methods to lose body weight in a short amount of time which poses a serious threat to athletes’ health and wellbeing. Therefore, the objective of this study was to investigate the most widespread rapid weight loss (RWL) methods and sources of influence used by grappling athletes.MethodsA total of 145 athletes took part in the study by voluntarily filling out a questionnaire regarding their weight loss techniques and methods. They were divided into two groups, male (27.7 ± 5.2 years, 1.76 ± 0.13 m, and 82.1 ± 20 kg) and female (27.33 ± 6.3 years, 1.65 ± 0.08 m, and 64.3 ± 10.4 kg), for further statistical analysis. After calculating descriptive statistics for all the variables, a t-test was conducted for gender differences in weight loss and weight regain, and a chi-square test measured the diversity in techniques and source of influence.ResultsOut of 145 participants, 120 athletes (85.2%) reported engaging in rapid weight loss prior to weigh-in. Coaches (52.4 and 59%) and teammates (42.6 and 22.1%) seemed to be the most influential on their rapid weight-loss strategies, whereas physicians (17.1 and 17.9%) and parents (23.2 and 23.1%) were the least influential. A statistical difference between men and women (p = 0.05) was found when teammates were a source of influence (42.6 and 21.1%, respectively). Regarding the methods used, both groups practiced gradual dieting (85.4 and 79.5%) as the most prevalent, followed by increased exercise (79 and 66.6%) and sauna (78.7 and 66.6%). Moreover, men trained in plastic suits significantly more often than women (67.1 vs. 41%, p = 0.01).ConclusionRapid weight loss is detrimental to athletes’ health and wellbeing. Hence, it is crucial to find and implement methods that will control and ultimately limit its use in combat sports. Physicians and nutritionists need to be closely linked with the staff, collaborate and supervise the weight cutting.
Are Psychiatrists Trained to Address the Mental Health Needs of Young People Transitioning From Child to Adult Services? Insights From a European Survey
Authors: Russet Frederick , Humbertclaude Veronique , Davidovic Vrljicak Nikolina , Dieleman Gwen C. , Dodig-Ćurković Katarina , Franic Tomislav , Gerritsen Suzanne E. , de Girolamo Giovanni , Hendrickx Gaelle , Kerbage Hala , McNicholas Fiona , Maras Athanasios , Paramala Santosh , Paul Moli , Schandrin Aurélie , Schulze Ulrike M. E. , Street Cathy , Tuomainen Helena , Wolke Dieter , Singh Swaran P. , Tremmery Sabine , Purper-Ouakil Diane 
Published: February 2022

BackgroundIn mental health, transition refers to the pathway of young people from child and adolescent to adult services. Training of mental health psychiatrists on transition-related topics offers the opportunity to improve clinical practice and experiences of young people reaching the upper age limit of child and adolescent care.MethodsNational psychiatrist's organizations or experts from 21 European countries were surveyed 1/ to describe the status of transition in adult psychiatry (AP) and child and adolescent psychiatry (CAP) postgraduate training in Europe; 2/ to explore the amount of cross-training between both specialties. This survey was a part of the MILESTONE project aiming to study and improve the transition process of young people at the service boundary.ResultsTransition was a mandatory topic in the AP curriculum of 1/19 countries (5%) and in the CAP curriculum of 4/17 countries (24%). Most topics relevant for transition planning were addressed during AP training in 7/17 countries (41%) to 10/17 countries (59%), and during CAP training in 9/11 countries (82%) to 13/13 countries (100%). Depending on the training models, theoretical education in CAP was mandatory during AP training in 94% (15/16) to 100% of the countries (3/3); and in AP during CAP training in 81% (13/16) to 100% of the countries (3/3). Placements were mandatory in CAP during AP training in 67% (2/3) to 71% of the countries (12/17); and in AP during CAP training in 87% (13/15) to 100% of the countries (3/3).Discussion and ConclusionSpecific training about transition is limited during CAP and AP postgraduate training in Europe. Cross-training between both specialties offers a basis for improved communication between child and adult services but efforts should be sustained in practical training. Recommendations are provided to foster further development and meet the specific needs of young people transitioning to adult services.
Paroxetine Repositioning – New Facets
Authors: Мангуби В.А. , Хаустова Е.А. 
Published: February 2022

В статье рассмотрены некоторые исследования, посвященные репозиционированию лекарственных препаратов, в частности пароксетина в области онкологии, а также комбинированная терапия с противоопухолевыми препаратами и применение пароксетина как препарата выбора при тревоге и депрессии у онкологических пациентов.
The article discusses some studies on the repositioning of drugs, in particular paroxetine, in the field of oncology. The combination therapy with anticancer drugs and the use of paroxetine as the drug of choice for anxiety and depression in cancer patients.
ЎҚУВ АДАБИЁТЛАРИНИНГ ТУРЛАРИ ВА УЛАРДА МАТННИ ИНФОГРАФИКА КЎРИНИШИДА БЕРИШ ИМКОНИЯТЛАРИ
Authors: IBRAGIMOVA Hilola 
Published: February 2022

Ахборот асрида маълумотлар сарҳадининг кенглиги аудиториянинг ахборот олишга бўлган талабини ҳам ўзгартирди. Бугун аудиториянинг алоҳида матн ёки одатий, овозли тасвир ва сўз уйғунлигидаги содда тузилишдаги материаллар билан чекланмай, ўзига хос қабул қилиниши осон, қулай, қийин ахборотни ҳам енгил, ҳам эсда қоларли ва кўркам тарзда олиш истаги юқори.	Бу талаб таъсирида ўқув адабиётларига бўлган талаб ҳам ўзгарди. Дарсликларга енгил ва тез ўзлаштириш имконини берувчи форматларни киритиш тақозо этилади. Шу жиҳатдан, эндиликда ўқув адабиётларини тайёрлашга янгича ёндашиш зарур.
Мазкур тадқиқотда биринчидан, ўқув адабиётларининг таркибий тузилиши ва турлари, электрон дарсликлар ва уларнинг ўзига хос жиҳатларини намоён этишида инфографиканинг ўрни каби масалалар ўрганилган ва таҳлил этилган. Иккинчидан, инфографика ўқув адабиётларини бойитиши, уни таъсирчанлигини ошириши ва дарсликни ўзлаштирилиши жиҳатдан соддалаштириш имконини бериши ҳақида фикр-мулоҳазалар келтирилган. Тадқиқот натижалари шуни кўрсатдики, инфографикани нафақат ўқув адабиётларида, ўз навбатида, дарс жараёнларида ҳам бир неча усулларда қўллаш самарали. Ўрганишлар натижаси инфографика ўқув адабиётлари, умуман, дарс жараёнларини визуаллаштириш, соддалаштириш ва қизиқарли қилишда жуда самарали формат эканлигини тасдиқлади.
Analisi di un risultato inatteso: le elezioni regionali del 2005 in Puglia
Authors: Giaffreda Marco 
Published: February 2022

Le elezioni regionali del 3-4 aprile 2005 in Puglia possono essere annoverate di diritto tra quelle di maggior interesse politologico sia per gli aspetti innovativi presenti, sia per il sorprendente esito. In una complessa tornata elettorale, per la prima volta in Puglia, un candidato alla carica di presidente della Regione è stato scelto attraverso elezioni primarie che hanno coinvolto la base dei partiti del centrosinistra, fornendo, come vedremo, una delle chiavi per il successo finale. Inoltre, dopo le elezioni del 2000, come del resto prevedeva il lungo processo di decentramento delle funzioni amministrative e di maggiore autonomia degli organi periferici dello Stato, si era aperta nella Regione una fase riformatrice che ha portato all’approvazione del nuovo Statuto regionale e di una nuova legge elettorale per l’elezione del presidente e del consiglio. Sul fronte strettamente elettorale i due principali candidati, Nichi Vendola per la Grande alleanza democratica (GAD), formata dall’Ulivo più Rifondazione comunista, e Raffaele Fitto, per la Casa delle Libertà (CDL), hanno dato vita ad una campagna fortemente personalizzata e speculare in cui sono emerse le profonde differenze tra i due e tra due modi (e concezioni) diversi di fare politica. La vittoria di Vendola, tutt’altro che scontata ma in qualche modo prevedibile, ha rappresentato, inoltre, un forte elemento di discontinuità rispetto al passato e alla tradizione politico-elettorale della Puglia. Per tutti questi motivi, quindi, sulle elezioni pugliesi si sono accesi i riflettori dei mass media nazionali.
Questo contributo ha l’obiettivo di descrivere e fornire una prima e parziale analisi del “terremoto pugliese”, dapprima attraverso una breve carrellata sulla storia del voto regionale in Puglia e sul contesto in cui si sono svolte le elezioni del 2005, per poi passare all’esame della nuova legge elettorale regionale, delle elezioni primarie nel centrosinistra e della loro influenza sull’esito finale della competizione, della campagna elettorale ed infine del risultato e delle possibili cause che l’hanno determinato.
La disciplina delle campagne elettorali nella prospettiva comparata: l'omaggio che il vizio rende alla virtù?
Authors: Fusaro Carlo 
Published: February 2022

Il 9 gennaio 2003 il primo ministro israeliano Ariel Sharon stava tenendo una conferenza stampa, ripresa in diretta radiotelevisiva, per difendersi da accuse di corruzione. Ma dopo nemmeno dieci minuti, il suo intervento veniva interrotto. Un annuncio comunicava subito dopo che la trasmissione era stata sospesa per ordine del presidente del Comitato elettorale centrale, giudice Mishael Cheshin, perché le parole di Sharon costituivano “propaganda elettorale”. Era infatti in svolgimento la campagna per le elezioni parlamentari del 28 gennaio e, durante questo periodo, la propaganda è vietata dalla legge in tutti i programmi su radio e tv tranne che negli appositi spazi a disposizione dei partiti.
Un’esagerazione? Un’assurdità? Forse. Questa certo può essere l’impressione a prima vista. Ma è opportuno, invece, sfuggire alla tentazione di subire una sorta di crisi di rigetto.
Questo lavoro presenta, con qualche osservazione generale, il quadro giuridico-formale all’interno del quale si sviluppano (o si dovrebbero oggi sviluppare) le campagne elettorali, intendendo per “campagne elettorali” tutte quelle attività specificamente volte a conquistare voti alle elezioni con particolare riferimento al periodo più o meno lungo (formalmente o sostanzialmente) che precede il giorno delle elezioni. Escludo la campagna elettorale referendaria che ha comunque caratteri propri. Vorrei escludere anche (ma alcune legislazioni lo includono, per cui qualcosa dovrò necessariamente dire al riguardo) il periodo di tempo che va dalle elezioni fino alla campagna elettorale successiva. È quello che la legge americana chiama electoral cycle, a indicare che in realtà il lavoro indirizzato alla conquista dei suffragi non si interrompe che per qualche ora, subito dopo un’elezione: ma ricomincia subito dopo in vista delle elezioni successive quando che siano. Ovviamente vi sono comunque nessi stretti con la disciplina dei partiti politici e, in particolare, la disciplina del loro finanziamento, nonché la disciplina dei mezzi di comunicazione di massa, in particolare del settore radiotelevisivo. Tuttavia, nessuna di queste materie è oggetto specifico di questo scritto, anche se non potrò evitare riferimenti anche frequenti. Pure stretti sono i nessi con la disciplina del sistema elettorale e con la struttura e il formato della lotta politica. Va da sé che queste delimitazioni hanno, come si dice in questi casi, carattere meramente stipulativo e nessuna pretesa di intrinseca validità.
Functional Results of Cephalomedullary Nail Osteosynthesis of Transtrochanteric Fractures in Elderly Patients
Authors: Sulyma V.S. , Valovina Yu.D. , Sabsai O.V. , Makarov V.B. , Valovina N.Yu. , Bihun R.R. , Kuz U.V. 
Published: February 2022

Summary. Relevance. Despite many surgical techniques that use modern metal fixators, treatment of elderly patients with fractures of the proximal part of the hip still remains relevant.
Objective: to evaluate the functional results of surgical treatment of elderly patients with transtrochanteric fractures of the femur using a cephalomedullary locking nail (PFNA).
Materials and Methods. The study involved 193 elderly patients (mean age 76.2±10.3 years) with transtrochanteric fractures of the femur type 31 A1-A2.1-1.3 (AO/OTA) after osteosynthesis by PFNA cephalomedullar fixator. The functional outcome of the treatment was determined after 1, 3, 12, and 36 months by Harris Hip Score (HHS), radiographic signs of bone union, and the presence of complications. The risk of recurrent trochanteric fractures over the next 10 years was calculated by adapted FRAX scale.
Results. Positive changes were found by HSS, in the dynamics: 51.6 after 1 month, 60.2 after 3 months, 79.7 after 12 months, and 84.4 after 36 months. Within 6 months after the surgery, the following changes were found: secondary displacement of bone fragments with the “cut-out” eruption of the cervical blade in 2.3% (n=3), migration of the cervical component outward in 4.7% (n=6), and inflammation of surgical wound in 3.9% (n=5) of cases. Retrospectively, the average FRAX score was assessed (22.12±1.76).
Conclusions. Stable functional osteosynthesis of transtrochanteric fractures of the femur using PFNA combined with early dosed loading on the injured limb allowed to obtain satisfactory functional results in 95.4% of cases. Secondary displacement of bone fragments with the “cut-out” effect requires a detailed analysis of all possible endo- and exogenous factors and identification of the most significant ones. The 10-year risk of recurrent fractures in patients with trochanteric fractures significantly increases over the age of 70, especially in females, and accounts for the majority of osteoporotic fractures.