bluuebunny/crossref_metadata_2025_split huggingface dataset:
MYTime: A Mindfulness and Yoga Program to Promote Health Outcomes in Parents of Children With Autism Spectrum Disorder
Authors: Ketcheson Leah R , Wengrovius Carissa M , Staples Kerri L , Miodrag Nancy 
Published: August 2022

Background Parenting a child with Autism Spectrum Disorder can be stressful and result in health consequences for the caregivers, a population that is often overlooked and underserved. While mind-body interventions such as mindfulness yoga can help promote positive health outcomes, limited research has explored the impact of such a program on caregivers of children on the autism spectrum. Objective The primary objective of this pilot study was to evaluate the effects of a 12-week mindfulness yoga program (MYtime) on perceived stress, anxiety, and depression among urban caregivers of children on the autism spectrum. Methods Twenty-seven parents were randomized into either MYTime (n = 13) or the control group (n = 14). Perceived Stress Scale (PSS) and Depression, Anxiety, and Stress Scale (DASS) were administered. Results The MYTime group reported lower stress, depression and anxiety while the control group maintained similar scores across pre- and post-assessment. Following 12 weeks of participation the PSS and DASS scores improved by 25% and 49% respectively for parents in the MYTime group. In comparison, PSS scores worsened by 5% and DASS scores improved by 17% for the Control group. Differences in pre-and post-assessment scores were not significant for either group ( P > .05). Conclusion The results from this pilot study indicate that a mindfulness yoga program could be considered among feasible interventions aimed at improving the health of parents of children on the autism spectrum.
Color Change Evaluation of Three Universal Resin Composites after Storage in Water: An In Vitro Study
Authors: Akgül Sinem , Gündoğdu Ceyda 
Published: August 2022

Aim: The aim of this in vitro study was to evaluate the color changes of three different universal resin composites after immersion in distilled water for one month. Materials and Methods: Omnichroma, Estelite Σ Quick, and Filtek Universal Restorative were investigated. Ten disk shaped specimens (6 mm diameter, 2 mm thickness) were prepared according to the manufacturers’ instructions for each material. After polishing, initial color measurements were taken followed by individual storage of the specimens in distilled water at 37 °C for one month when color measurements were repeated. The change in the color of each resin composites (Δ E00) was calculated with a digital method, using the CIEDE2000 color difference formula. The data were analyzed using one-way ANOVA followed by Tukey post-hoc test ( P < .05). Results: All resin composites exhibited acceptable color change after one month. Omnichroma exhibited the highest color change (2.26 ± 0.48) value while Filtek Universal Restorative showed the lowest (1.14 ± 0.38) value. All color parameters influenced the Δ E00 values in all groups except Filtek Universal for Δ L* and Δ b values. Conclusion: When performing composite resin restorations, especially for esthetic purposes, polymerization may lead to change in the color of restorative material over time, but it should be considered whether this is within acceptable limits.
Arthroscopic Posterior Glenoid Augmentation With Distal Tibial Allograft
Authors: Dean Robert S. , Dennis Eric J. , Torres LeeAnne F. , Rider Danielle E. , Trasolini Nicholas A. , Gehrman Max D. , Waterman Brian R. 
Published: August 2022

Background: Posterior glenohumeral instability is much less common than anterior instability, and there is a paucity of studies looking at glenoid bone loss as it relates to posterior instability. However, while the data are not as robust, posterior glenoid bone loss can lead to recurrent instability and failed soft tissue procedures. Arthroscopic posterior glenoid augmentation with distal tibial allograft (DTA) is a minimally invasive option to restore stability and preserve function. Indications: The primary indication for posterior glenoid augmentation is posterior instability with >20% to 25% posterior glenoid bone loss or recurrent posterior instability after prior stabilization procedure. In this case, the patient is a 21-year-old man with recurrent instability after 2 prior soft tissue stabilization procedures. Technique Description: The patient was positioned in lateral decubitus, and portals were established. Arthroscopic evaluation was performed to assess the labrum, biceps, rotator cuff, glenoid, and humeral head. Glenoid mobilization was performed, and an incision was made for introduction of the bone block. The glenoid was prepared, and a trial was used to guide preparation of the graft, which was harvested from the articular cartilage of the distal tibia. The graft was irrigated and bathed in platelet-rich plasma (PRP) and then introduced and positioned for maximal coverage of the defect. Screw fixation was performed with two 3.75-mm screws. The posterior capsule was reapproximated, and a layered closure was performed. Results: Previous studies have reported significant improvements in patient-reported outcomes, high rates of healing, and no cases of recurrent instability after DTA for anterior glenoid bone loss. Additional studies have reported few patients with recurrent instability and no instances of partial or non-union. Significant loss of range of motion has not been reported in the most recent case series. One previous study reported significantly improved patient-reported outcomes and near-complete osseous reabsorption with DTA after failed Latarjet procedure. Discussion/Conclusion: Arthroscopic posterior glenoid augmentation with DTA is a viable treatment option for patients with shoulder pain and instability with >20% to 25% posterior glenoid bone loss and/or following prior stabilization procedures.
Differential DNA methylation in Pacific oyster reproductive tissue in response to ocean acidification
Authors: Venkataraman Yaamini R. , White Samuel J. , Roberts Steven B. 
Published: August 2022

AbstractBackgroundThere is a need to investigate mechanisms of phenotypic plasticity in marine invertebrates as negative effects of climate change, like ocean acidification, are experienced by coastal ecosystems. Environmentally-induced changes to the methylome may regulate gene expression, but methylome responses can be species- and tissue-specific. Tissue-specificity has implications for gonad tissue, as gonad-specific methylation patterns may be inherited by offspring. We used the Pacific oyster (Crassostrea gigas)— a model for understanding pH impacts on bivalve molecular physiology due to its genomic resources and importance in global aquaculture— to assess how low pH could impact the gonad methylome. Oysters were exposed to either low pH (7.31 ± 0.02) or ambient pH (7.82 ± 0.02) conditions for 7 weeks. Whole genome bisulfite sequencing was used to identify methylated regions in female oyster gonad samples. C- > T single nucleotide polymorphisms were identified and removed to ensure accurate methylation characterization.ResultsAnalysis of gonad methylomes revealed a total of 1284 differentially methylated loci (DML) found primarily in genes, with several genes containing multiple DML. Gene ontologies for genes containing DML were involved in development and stress response, suggesting methylation may promote gonad growth homeostasis in low pH conditions. Additionally, several of these genes were associated with cytoskeletal structure regulation, metabolism, and protein ubiquitination — commonly-observed responses to ocean acidification. Comparison of these DML with otherCrassostreaspp. exposed to ocean acidification demonstrates that similar pathways, but not identical genes, are impacted by methylation.ConclusionsOur work suggests DNA methylation may have a regulatory role in gonad and larval development, which would shape adult and offspring responses to low pH stress. Combined with existing molluscan methylome research, our work further supports the need for tissue- and species-specific studies to understand the potential regulatory role of DNA methylation.
Accumulation and regulation of anthocyanins in white and purple Tibetan Hulless Barley (Hordeum vulgare L. var. nudum Hook. f.) revealed by combined de novo transcriptomics and metabolomics
Authors: Yao Xiaohua , Yao Youhua , An Likun , Li Xin , Bai Yixiong , Cui Yongmei , Wu Kunlun 
Published: August 2022

AbstractBackgroundColored barley, which may have associated human health benefits, is more desirable than the standard white variety, but the metabolites and molecular mechanisms underlying seedcoat coloration remain unclear.ResultsHere, the development of Tibetan hulless barley was monitored, and 18 biological samples at 3 seedcoat color developmental stages were analyzed by transcriptomic and metabolic assays in Nierumuzha (purple) and Kunlun10 (white). A total of 41 anthocyanin compounds and 4186 DEGs were identified. Then we constructed the proanthocyanin-anthocyanin biosynthesis pathway of Tibetan hulless barley, including 19 genes encoding structural enzymes in 12 classes (PAL,C4H,4CL,CHS,CHI,F3H,F3’H,DFR,ANS,ANR,GT, andACT). 11 DEGs other thanANRwere significantly upregulated in Nierumuzha as compared to Kunlun10, leading to high levels of 15 anthocyanin compounds in this variety (more than 25 times greater than the contents in Kunlun10).ANRwas significantly upregulated in Kunlun10 as compared to Nierumuzha, resulting in higher contents of three anthocyanins compounds (more than 5 times greater than the contents in Nierumuzha). In addition, 22 TFs, includingMYBs,bHLHs,NACs,bZips, andWD40s, were significantly positively or negatively correlated with the expression patterns of the structural genes. Moreover, comparisons of homologous gene sequences between the two varieties identified 61 putative SNPs in 13 of 19 structural genes. A nonsense mutation was identified in the coding sequence of theANSgene in Kunlun10. This mutation might encode a nonfunctional protein, further reducing anthocyanin accumulation in Kunlun10. Then we identified 3 modules were highly specific to the Nierumuzha (purple) using WGCNA. Moreover, 12 DEGs appeared both in the putative proanthocyanin-anthocyanin biosynthesis pathway and the protein co-expression network were obtained and verified.ConclusionOur study constructed the proanthocyanin-anthocyanin biosynthesis pathway of Tibetan hulless barley. A series of compounds, structural genes and TFs responsible for the differences between purple and white hulless barley were obtained in this pathway. Our study improves the understanding of the molecular mechanisms of anthocyanin accumulation and biosynthesis in barley seeds. It provides new targets for the genetic improvement of anthocyanin content and a framework for improving the nutritional quality of barley.
Moderate sedation with single-dose remimazolam tosilate in elderly male patients undergoing transurethral resection of the prostate with spinal anesthesia: a prospective, single-arm, single-centre clinical trial
Authors: Zhao Tang-yuan-meng , Chen Di , Sun Hu , Xu Zhi-xin , Lyu Song , Wang Tao , Liu Li-li 
Published: August 2022

Abstract
Background
Remimazolam tosilate (RT) is a newly listed benzodiazepine for sedation and anesthesia featuring quick onset of effects, short maintenance and recovery times, which is currently under research. This trial was conducted to determine the median effective dose (ED50) and the 95% effective dose (ED95) of single-dose remimazolam for moderate sedation in elderly patients undergoing transurethral resection of the prostate (TURP) under spinal anesthesia, and to evaluate its efficacy and safety.

Methods
Thirty male patients aged 65–80 years old were recruited for selective TURP. Remimazolam was administered intravenously to pain-free patients (VAS score < 1) within 1 min of successful spinal anesthesia by the same anesthesiologist. We used modified Dixon’s up-and-down sequential allocation method to determine the ED50 and ED95 of the agent with an initial dosage of 0.1 mg/kg. Successful sedation was defined as an MOAA/S score ≤ 3 and above 1. A score of > 3 was deemed as failed sedation. Recruitment continued until ten independent pairs (from successful sedation to failed sedation) would give a reliable estimation of the ED50 and ED95 of RT and their 95% confidence intervals.

Results
The ED50 of remimazolam was 0.063 (95% C.I. 0.045–0.073) mg/kg. Its ED95 was 0.079 (95% C.I. 0.07–0.137) mg/kg. Remimazolam was safe in its application.

Conclusions
A single-dose of RT proves to be safe for assisted sedation during TURP in elderly male patients under spinal anesthesia with a lower incidence of adverse events. Its ED50 and ED95 were 0.063 mg/kg and 0.079 mg/kg, respectively.

Trial registration
http://www.chictr.org.cn (ChiCTR2100051912).
Catastrophic health expenditure among Chinese adults living alone with cognitive impairment: findings from the CHARLS
Authors: Li Chenxi , Jin Shuyi , Cao Xingqi , Han Ling , Sun Ning , Allore Heather , Hoogendijk Emiel O. , Xu Xin , Feng Qiushi , Liu Xiaoting , Liu Zuyun 
Published: August 2022

Abstract
Background
The catastrophic health expenditure of older adults results in serious consequences; however, the issue of whether cognitive status and living situations contribute to such financial burdens is uncertain. Our aim was to compare the differences in catastrophic health expenditure between adults living alone with cognitive impairment and those adults living with others and with normal cognition.

Methods
We identified 909 observations of participants living alone with cognitive impairment (cases) and 37,432 observations of participants living with others and with normal cognition (comparators) from the 2011/2012, 2013, 2015 and 2018 waves of the China Health and Retirement Longitudinal Study (CHARLS). We used propensity score matching (1:2) to create matched cases and comparators in a covariate-adjusted logistic regression analysis. Catastrophic health expenditure was defined as an out-of-pocket cost for health care ≥40% of a household’s capacity to pay.

Results
In comparison with participants living with others and with normal cognition, those adults living alone with cognitive impairment reported a higher percentage of catastrophic health expenditure (19.5% vs. 11.8%, respectively, P < 0.001). When controlling for age, sex, education, marital status, residence areas, alcohol consumption, smoking status and disease counts, we found that this subpopulation had significantly higher odds of having catastrophic health expenditure (odds ratio [OR] = 1.89, 95% confidence interval [CI]: 1.40, 2.56). Additional analyses confirmed the robustness of the results.

Conclusions
This study demonstrated that adults living alone with cognitive impairment in the CHARLS experienced a high burden of catastrophic health expenditure. Health care policies on social health insurance and medical assistance should consider these vulnerable adults.
Effect of an exercise intervention on global cognition after transient ischemic attack or minor stroke: the MoveIT randomized controlled trial
Authors: Deijle Inger A. , Hemmes Roelofjan , Boss H. Myrthe , de Melker Edwin C. , van den Berg Bob T. J. , Kwakkel Gert , van Wegen Erwin , Bosboom Wendy M. , Weinstein Henry C. , van Schaik Sander M. , Van den Berg-Vos Renske M. 
Published: August 2022

Abstract
Background
Patients with a transient ischemic attack (TIA) or ischemic stroke are at increased risk of developing cognitive impairment in the subacute phase. At present, the effects of exercise on cognitive functioning following a TIA or stroke are not fully known. The purpose of this trial was to investigate the effect of exercise on global cognition.

Methods
The MoveIT trial is a single-centre, observer-blinded, randomized controlled trial involving a 1-year exercise intervention consisting of a 12-week group exercise program, combined with three counselling visits to the physiotherapists over a 9-month period. The control group received standard care. The primary outcome was global cognitive functioning, assessed at one year, using the Montreal Cognitive Assessment (MoCA). Secondary outcomes included cardiorespiratory fitness, the cardiovascular profile, and attainment of secondary prevention targets, anxiety, depression and fatigue at one and two years.

Results
The experimental group consisted of 60 patients, while the control group consisted of 59 patients. The mean age was 64.3 years and 41% were female. No between-group differences were found on global cognitive functioning (MD, 0.7 out of 30, 95% CI, − 0.2 to 1.6) or on secondary outcome measures at 12 months. The only significant between-group difference was found for fatigue, in favour of the experimental group at 12 months (MD, 0.6 out of 63, 95% CI, 0.1 to 1.1).

Conclusions
No benefit of this exercise intervention was found regarding global cognition. Future studies need to focus on optimizing rehabilitation strategies for this vulnerable group of patients.

Trial registration
http://www.trialregister.nl. Unique identifier: NL3721.
Date first registration: 06-03-2013.
Self-reported vomiting during pregnancy in North-east Nigeria: perceptions, prevalence, severity and impacts
Authors: Yargawa Judith , Hill Zelee , Fottrell Edward 
Published: August 2022

Abstract
Background
Vomiting is a common ailment during pregnancy, often linked to negative impacts on women’s quality of life. Very little is known about the issue in low income settings, particularly Sub-Saharan Africa, with studies from high income settings predominantly informing the evidence base. This study aimed to explore how women perceive vomiting during pregnancy and to measure its prevalence, severity and impacts in North-east Nigeria.

Methods
Qualitative in-depth interviews, focus group discussions, family interviews and a cross-sectional household survey were carried out between December 2015 and November 2016 with women who had given birth within the past two years. Purposive sampling and thematic analysis were used in the qualitative studies. A three-staged cluster sampling with 640 women and descriptive analyses were used in the survey.

Results
Women in the qualitative studies reported that vomiting was a normal part of pregnancy, unless a woman vomits after eating, has poor appetite, is not well-nourished, cannot perform chores, is overwhelmed by it or has to go to hospital. In the survey, 35.4% (95% CI 26.5–45.5) of women reported any vomiting during their last pregnancies and of these only 21.1% said it had stopped entirely within the first trimester. Over half of women who reported vomiting did so at least three times per day most days and 34.7% were vomiting five or more times per day during the most severe period. Care-seeking was reported by 61.5%. Both the qualitative and quantitative data found that vomiting impacted women in multiple ways including nutritionally, physiologically, mentally, financially and martially; 50.8% of women with any vomiting in the survey perceived the overall severity of the condition negatively.

Conclusion
Vomiting during pregnancy is dually seen as normal and problematic depending on its characteristics and impacts. The burden appears to be high with many women seeking care for the condition.
Neoadjuvant nivolumab + T-VEC combination therapy for resectable early stage or metastatic (IIIB-IVM1a) melanoma with injectable disease: study protocol of the NIVEC trial
Authors: Rohaan Maartje W. , Stahlie Emma H. A. , Franke Viola , Zijlker Lisanne P. , Wilgenhof Sofie , van der Noort Vincent , van Akkooi Alexander C. J. , Haanen John B. A. G. 
Published: August 2022

Abstract
Background
Trials investigating neoadjuvant treatment with immune checkpoint inhibitors (ICI) in patients with melanoma have shown high clinical and pathologic response rates. Treatment with talimogene laherparepvec (T-VEC), a modified herpes simplex virus type-1 (HSV-1), is approved for patients with unresectable stage IIIB-IVM1a melanoma and has the potential to make tumors more susceptible for ICI. Combination ICI and intralesional T-VEC has already been investigated in patients with unresectable stage IIIB-IV disease, however, no data is available yet on the potential benefit of this combination therapy in neoadjuvant setting.

Methods
This single center, single arm, phase II study aims to show an improved major pathologic complete response (pCR) rate, either pCR or near-pCR, up to 45% in 24 patients with resectable stage IIIB-IVM1a melanoma upon neoadjuvant combination treatment with intralesional T-VEC and systemic nivolumab (anti-PD-1 antibody). Patients will receive four courses of T-VEC up to 4 mL (first dose as seroconversion dose) and three doses of nivolumab (240 mg flatdose) every 2 weeks, followed by surgical resection in week nine. The primary endpoint of this trial is pathologic response rate. Secondary endpoints are safety, the rate of delay of surgery and event-free survival. Additionally, prognostic and predictive biomarker research and health-related quality of life evaluation will be performed.

Discussion
Intralesional T-VEC has the capacity to heighten the immune response and to elicit an abscopal effect in melanoma in combination with ICI. However, the potential clinical benefit of T-VEC plus ICI in the neoadjuvant setting remains unknown. This is the first trial investigating the efficacy and safety of neoadjuvant treatment of T-VEC and nivolumab followed by surgical resection in patients with stage IIIB-IVM1a melanoma, with the potential of high pathologic response rates and acceptable toxicity.

Trial registration
This trial was registered in the European Union Drug Regulating Authorities Clinical Trials Database (EudraCT- number: 2019–001911-22) and the Central Committee on Research Involving Human Subjects (NL71866.000.19) on 4th June 2020.
Secondary identifying number: NCT04330430.
Association between metabolic syndrome, C-reactive protein, and the risk of primary liver cancer: a large prospective study
Authors: Song Mengmeng , Liu Tong , Liu Hai , Zhang Qi , Zhang Qingsong , Wang Yiming , Ma Xiangming , Cao Liying , Shi Hanping 
Published: August 2022

AbstractBackground and aimsHigh-sensitivity C-reactive protein (hs-CRP) levels and metabolic syndrome (MetS) are known to be associated with an increased incidence of different cancers. We aimed to evaluate the effect of MetS combined with high hs-CRP levels on the risk of primary liver cancer (PLC).MethodsParticipants were recruited from the Kailuan cohort study and were classified into four groups according to the presence or absence of MetS and inflammation (hs-CRP ≥ 3 or < 3 mg/L). The associations of MetS and inflammation with the risk of PLC were assessed using Cox proportional hazards models.ResultsThis study included 92,770 participants. The mean age was 51.4 years old. Over a median follow-up of 13.02 years, 395 participants were diagnosed as PLC. Compared to the control participants without inflammation (hs-CRP < 3 mg/L) and MetS (n = 69,413), participants with high hs-CRP levels combined with MetS (n = 2,269) had a higher risk of PLC [hazard ratios (HR) 2.91; 95% confidence interval (CI), 1.77–4.81], and participants with high hs-CRP levels and without MetS (n = 14,576) had the same trend (HR, 1.36; 95%CI, 1.05–1.75). However, participants with low hs-CRP levels and MetS (n = 6,512) had no significant association with an elevated risk of PLC (HR, 1.18; 95%CI, 0.76–1.82). After excluding participants who had cancer during the first year of follow-up, sensitivity analysis showed the same trend. In addition, co-occurrence of MetS and high hs-CRP levels had significant interactive effects on the risk of PLC between the sexes (P < 0.001) and the patients with HBV infection (P = 0.012).ConclusionsParticipants with co-occurrence of MetS and high hs-CRP levels have an elevated risk of PLC.Trial registrationKailuan study, ChiCTR–TNRC–11001489. Registered 24 August, 2011-Retrospectively registered,http://www.chictr.org.cn/showprojen.aspx?proj=8050