bluuebunny/crossref_metadata_2025_split huggingface dataset:
Superinfections caused by carbapenem-resistant Enterobacterales in hospitalized patients with COVID-19: a multicentre observational study from Italy (CREVID Study)
Authors: Falcone Marco , Suardi Lorenzo Roberto , Tiseo Giusy , Galfo Valentina , Occhineri Sara , Verdenelli Stefano , Ceccarelli Giancarlo , Poli Melita , Merli Marco , Bavaro Davide , Carretta Anna , Nunnari Giuseppe , Venanzi Rullo Emmanuele , Trecarichi Enrico Maria , Papalini Chiara , Franco Antonina , Del Vecchio Rosa Fontana , Bianco Vincenzo , Punzi Rodolfo , Francisci Daniela , Rubino Raffaella , Torti Carlo , Puoti Massimo , Carbonara Sergio , Cascio Antonio , Saracino Annalisa , Santantonio Teresa , Venditti Mario , Menichetti Francesco 
Published: June 2022

Abstract

Objectives
To describe clinical characteristics and outcomes of COVID-19 patients who developed secondary infections due to carbapenem-resistant Enterobacterales (CRE).

Methods
Retrospective observational study including COVID-19 patients admitted to 12 Italian hospitals from March to December 2020 who developed a superinfection by CRE. Superinfection was defined as the occurrence of documented bacterial infection &gt;48 h from admission. Patients with polymicrobial infections were excluded. Demographic, clinical characteristics and outcome were collected. Isolates were classified as KPC, metallo-β-lactamase (MBL) and OXA-48-producing CRE. A Cox regression analysis was performed to identify factors independently associated with 30 day mortality.

Results
Overall, 123 patients (median age 66 years, IQR 59–75) were included. The majority of infections occurred in the ICU (81, 65.9%), while 42 (34.1%) in medical wards. The most common types of infection were bloodstream infections (BSI) (n = 64, 52%), followed by urinary-tract infections (UTI) (n = 28, 22.8%), hospital-acquired/ventilator-associated pneumonia (HAP/VAP) (n = 28, 22.8%), intra-abdominal infections (n = 2, 1.6%) and skin infections (n = 1, 0.8%). Sixty-three (51.2%) infections were caused by KPC-, 54 (43.9%) by MBL-, and 6 (4.8%) by OXA-48-producing CRE. Thirty-day mortality was 33.3% (41/123). On Cox regression analysis, HAP/VAP compared with UTI (HR 7.23, 95% CI 2.09–24.97, P = 0.004), BSI compared with UTI (HR 3.96, 95% CI, 1.33–11.77, P = 0.004), lymphopenia on admission (HR 3, 95% CI 1.44–6.26, P = 0.003) and age (HR 1.05, 95% CI 1.02–1.08, P = 0.002) were predictors of 30 day mortality.

Conclusions
Superinfections by CRE were associated with high risk of 30 day mortality in patients with COVID-19. HAP/VAP was the strongest predictor of death in these patients.
Impact of housing environment and management on pre-/post-weaning piglet productivity
Authors: Ramirez Brett C , Hayes Morgan D , Condotta Isabella C F S , Leonard Suzanne M 
Published: June 2022

AbstractThe complex environment surrounding young pigs reared in intensive housing systems directly influences their productivity and livelihood. Much of the seminal literature utilized housing and husbandry practices that have since drastically evolved through advances in genetic potential, nutrition, health, and technology. This review focuses on the environmental interaction and responses of pigs during the first 8 wk of life, separated into pre-weaning (creep areas) and post-weaning (nursery or wean-finish) phases. Further, a perspective on instrumentation and precision technologies for animal-based (physiological and behavioral) and environmental measures documents current approaches and future possibilities. A warm microclimate for piglets during the early days of life, especially the first 12 h, is critical. While caretaker interventions can mitigate the extent of hypothermia, low birth weight remains a dominant risk factor for mortality. Post-weaning, the thermoregulation capabilities have improved, but subsequent transportation, nutritional, and social stressors enhance the requisite need for a warm, low draft environment with the proper flooring. A better understanding of the individual environmental factors that affect young pigs as well as the creation of comprehensive environment indices or improved, non-contact sensing technology is needed to better evaluate and manage piglet environments. Such enhanced understanding and evaluation of pig–environment interaction could lead to innovative environmental control and husbandry interventions to foster healthy and productive pigs.
Review: Physiology and nutrition of late gestating and transition sows
Authors: Theil Peter Kappel , Farmer Chantal , Feyera Takele 
Published: June 2022

AbstractThe physiology during late gestation and the transition period to lactation changes dramatically in the sow, especially during the latter period. Understanding the physiological processes and how they change dynamically as the sow approaches farrowing, nest building, giving birth to piglets, and producing colostrum is important because these processes greatly affect sow productivity. Glucose originating from assimilated starch accounts for the majority of dietary energy, and around farrowing, various organs and peripheral tissues compete for plasma glucose, which may become depleted. Indeed, physical activity increases shortly prior to farrowing, leading to glucose use by muscles. Approximately ½ to 1 d later, glucose is also needed for uterine contractions to expel the piglets and for the mammary gland to produce lactose and fat for colostrum. At farrowing, the sow appears to prioritize glucose to the mammary gland above the uterus, whereby insufficient dietary energy may compromise the farrowing process. At this time, energy metabolism in the uterus shifts dramatically from relying mainly on the oxidation of glucogenic energy substrates (primarily glucose) to ketogenic energy supplied from triglycerides. The rapid growth of mammary tissue occurs in the last third of gestation, and it accelerates as the sow approaches farrowing. In the last 1 to 2 wk prepartum, some fat may be produced in the mammary glands and stored to be secreted in either colostrum or transient milk. During the first 6 h after the onset of farrowing, the uptake of glucose and lactate by the mammary glands roughly doubles. Lactate is supplying approximately 15% of the glucogenic carbon taken up by the mammary glands and originates from the strong uterine contractions. Thereafter, the mammary uptake of glucose and lactate declines, which suggests that the amount of colostrum secreted starts to decrease at that time. Optimal nutrition of sows during late gestation and the transition period should focus on mammary development, farrowing performance, and colostrum production. The birth weight of piglets seems to be only slightly responsive to maternal nutrition in gilts; on the other hand, sows will counterbalance insufficient feed or nutrient intake by increasing mobilization of their body reserves. Ensuring sufficient energy to sows around farrowing is crucial and may be achieved via adequate feed supply, at least three daily meals, high dietary fiber content, and extra supplementation of energy.
Genetic aspects of piglet survival and related traits: a review
Authors: Knol Egbert F , van der Spek Dianne , Zak Louisa J 
Published: June 2022

AbstractIn livestock, mortality in general, and mortality of the young, is societal worries and is economically relevant for farm efficiency. Genetic change is cumulative; if it exists for survival of the young and genetic merit can be estimated with sufficient accuracy, it can help alleviate the pressure of mortality. Lack of survival is a moving target; livestock production is in continuous change and labor shortage is a given. There is now ample evidence of clear genetic variance and of models able to provide genomic predictions with enough accuracy for selection response. Underlying traits such as birth weight, uniformity in birth weight, gestation length, number of teats, and farrowing duration all show genetic variation and support selection for survival or, alternatively, be selected for on their own merit.
Functional Reconstruction for Severe Heat Press Injury of the Proper Digits Using Immediate Preserved Subdermal Vascular Network Skin Graft: A Case Report
Authors: Hihara Masakatsu , Kakudo Natsuko , Mitsui Toshihito , Kuro Atsuyuki , Kusumoto Kenji 
Published: June 2022

Abstract
The patient was a 57-year-old woman who suffered third-degree burns to the dorsal side of her left fingers (non-dominant hand) when it was caught in a press machine heated to 200°C while she was cleaning clothes. At 20 hours after the injury, the necrotic tissue was excised and covered with a preserved subdermal vascular network (PSVN) skin graft, which allowed the patient to regain a very good hand function. Even for severe heat press injuries of the hand, the combination of immediate surgical intervention, a thick skin graft with PSVN, and early rehabilitation from the first postoperative week leads to good functional and cosmetic reconstruction outcomes.
Exploring the Insecticidal Potential of Gaseous and Aqueous Ozone to Control Spotted-Wing Drosophila,Drosophila suzukii(Diptera: Drosophilidae)
Authors: Savage Benjamin A , Masten Susan , Chung Henry , Grieshop Matthew 
Published: June 2022

AbstractOver the last decade, numerous companies have marketed aqueous ozone sprayers for insect and disease management, but little to no data has been published on their efficacy. Thus, we evaluated the potential of both gaseous and aqueous ozone as a potential preharvest insecticide against the adult life stage of the invasive fruit pest, spotted-wing drosophila, Drosophila suzukii (Matsumura). Gaseous ozone was applied at two dosages, 14,600 and 30,100 ppmv, for varying durations and the respective concentration-time (CT) exposure responses were modeled for sex-specific mortalities recorded at 0, 24, 48, and 72 h following treatment. We found that gaseous ozone primarily caused mortality immediately following exposure, with slight increases 72 h following ozone treatments. The female and male lethal concentration-time (LCT) 50 estimates were significantly different at 0, 24, 48, and 72 h after 30,100 ppmv treatments, where males observed an increased mortality response. However, the LCT 99 estimates confidence intervals (95%) of adult female and male D. suzukii were similar at 0, 24, 48, and 72 h after 14,600 or 30,100 ppmv ozone treatments. In contrast, ozone dissolved in distilled water at 18.52 ppm (mg/L) did not provide any mortality after total immersion of subjects for 30 s. While gaseous ozone may have some utility as a fumigant for D. suzukii in closed vessels where concentrations could be maintained, we did not identify any insecticidal potential for ozone dissolved in aqueous solution when simulating a preharvest treatment under optimal laboratory conditions.
A Near-Deterministic Mutational Hotspot in Pseudomonas fluorescens Is Constructed by Multiple Interacting Genomic Features
Authors: Shepherd M J , Horton J S , Taylor T B 
Published: June 2022

Abstract
Mutation—whilst stochastic—is frequently biased toward certain loci. When combined with selection, this results in highly repeatable and predictable evolutionary outcomes. Immotile variants of the bacterium Pseudomonas fluorescens (SBW25) possess a “mutational hotspot” that facilitates repeated occurrences of an identical de novo single nucleotide polymorphism when re-evolving motility, where ≥95% independent lines fix the mutation ntrB A289C. Identifying hotspots of similar potency in other genes and genomic backgrounds would prove valuable for predictive evolutionary models but to do so we must understand the genomic features that enable such a hotspot to form. Here, we reveal that genomic location, local nucleotide sequence, gene strandedness, and presence of mismatch repair proteins operate in combination to facilitate the formation of this mutational hotspot. Our study therefore provides a framework for utilizing genomic features to predict and identify hotspot positions capable of enforcing near-deterministic evolution.
The retrograde signaling regulator ANAC017 recruits the MKK9–MPK3/6, ethylene, and auxin signaling pathways to balance mitochondrial dysfunction with growth
Authors: He Cunman , Liew Lim Chee , Yin Lingling , Lewsey Mathew G , Whelan James , Berkowitz Oliver 
Published: June 2022

Abstract
In plant cells, mitochondria are ideally positioned to sense and balance changes in energy metabolism in response to changing environmental conditions. Retrograde signaling from mitochondria to the nucleus is crucial for adjusting the required transcriptional responses. We show that ANAC017, the master regulator of mitochondrial stress, directly recruits a signaling cascade involving the plant hormones ethylene and auxin as well as the MAP KINASE KINASE (MKK) 9–MAP KINASE (MPK) 3/6 pathway in Arabidopsis thaliana. Chromatin immunoprecipitation followed by sequencing and overexpression demonstrated that ANAC017 directly regulates several genes of the ethylene and auxin pathways, including MKK9, 1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2, and YUCCA 5, in addition to genes encoding transcription factors regulating plant growth and stress responses such as BASIC REGION/LEUCINE ZIPPER MOTIF (bZIP) 60, bZIP53, ANAC081/ATAF2, and RADICAL-INDUCED CELL DEATH1. A time-resolved RNA-seq experiment established that ethylene signaling precedes the stimulation of auxin signaling in the mitochondrial stress response, with a large part of the transcriptional regulation dependent on ETHYLENE-INSENSITIVE 3. These results were confirmed by mutant analyses. Our findings identify the molecular components controlled by ANAC017, which integrates the primary stress responses to mitochondrial dysfunction with whole plant growth via the activation of regulatory and partly antagonistic feedback loops.
Perceptions and Experiences of Individuals With Neck Pain: A Systematic Critical Review of Qualitative Studies With Meta-Summary and Meta-Synthesis
Authors: Falsiroli Maistrello Luca , Zanconato Laura , Palese Alvisa , Geri Tommaso , Turolla Andrea , Gianola Silvia , Dell’Isola Andrea , Rossettini Giacomo 
Published: June 2022

Abstract

Objective
This study aimed at exploring the perspectives and experiences of individuals with neck pain by synthesizing all available qualitative studies.

Methods
A systematic, qualitative meta-summary and meta-synthesis was conducted following the 5-step methodology proposed by Sandelowski and Barroso. A systematic search of 9 electronic databases was conducted in May 2021. Methodological quality was assessed using the Critical Appraisal Skills Programme tool.

Results
Nine studies were included in the meta-synthesis for a total of 103 participants (73 women). Eleven categories were identified as belonging to 3 main themes: physical (“My neck has gone wrong”); psychological (“I am worried about my recovery”); and social (“Pain limits my life”). In the physical theme, the categories with the highest frequency were symptoms (67%) followed by body perception (44%). The psychological theme frequencies from highest to lowest were psychological consequences (100%), coping strategies (100%), mindset (67%), expectations from health care professionals (44%), and gender influence (22%). In the social theme, frequencies from highest to lowest were social relationships (56%), work, and activities of daily living and physical activity (44%, respectively).

Conclusion
An individual’s experience with neck pain is a multidimensional phenomenon in which physical, psychological, and social dimensions influence each other. These findings suggested that health care professionals should be aware of recognizing and evaluating all of the individual’s experiences to offer a truly patient-centered care pathway.

Impact
This qualitative meta-synthesis responded to a call to action to explore perspectives and experiences of individuals with neck pain. These findings can provide crucial guidance for clinicians as they plan and implement evidence-based recommendations for neck pain.
Plastid development of albino viviparous propagules in the woody mangrove species of Kandelia obovata
Authors: Hao Saiqi , Hu Wei , Ye Congting , Shen Yingjia , Li Qingshun Q 
Published: June 2022

Abstract
The process of plastids developing into chloroplasts is critical for plants to survive. However, this process in woody plants is less understood. Kandelia obovata Sheue, Liu & Yong is a viviparous mangrove species; the seeds germinate on the maternal tree, and the hypocotyls continue to develop into mature propagules. We identified rare albino propagules through field observation among normal green and brown ones. Toward unveiling the propagule plastid development mechanism, albino propagule leaves only have etioplasts, low photosynthesis rates, and drastically reduced chlorophyll a/b and carotenoid contents, but with increased superoxide dismutase activities. To identify candidate genes controlling propagule plastid development, a genome-wide association study (GWAS) was performed between the albino and green propagules. Twenty-five significant single nucleotide polymorphisms (SNPs) were associated with albino propagule plastid development, the most significant SNPs being located on chromosomes 1 and 5. Significant differentially expressed genes were identified in porphyrin and chlorophyll metabolisms, carotenoid and flavonoid biosynthesis by combining transcriptome and GWAS data. In particular, KoDELLAs, encoding a transcription factor and KoCHS, encoding chalcone synthase, may be essential to regulate the albino propagules plastid development through weakened chlorophyll and flavonoid biosynthesis pathways while promoting chlorophyll degradation. Our results provide insights into genetic mechanisms regulating propagule plastid development in woody plants.
Limited acclimation of leaf traits and leaf temperatures in a subtropical urban heat island
Authors: Kullberg Alyssa T , Feeley Kenneth J 
Published: June 2022

Abstract
The consequences of rising temperatures for trees will vary between species based on their abilities to acclimate their leaf thermoregulatory traits and photosynthetic thermal tolerances. We tested the hypotheses that adult trees in warmer growing conditions (i) acclimate their thermoregulatory traits to regulate leaf temperatures, (ii) acclimate their thermal tolerances such that tolerances are positively correlated with leaf temperature and (iii) that species with broader thermal niche breadths have greater acclimatory abilities. To test these hypotheses, we measured leaf traits and thermal tolerances of seven focal tree species across steep thermal gradients in Miami’s urban heat island. We found that some functional traits varied significantly across air temperatures within species. For example, leaf thickness increased with maximum air temperature in three species, and leaf mass per area and leaf reflectance both increased with air temperature in one species. Only one species was marginally more homeothermic than expected by chance due to acclimation of its thermoregulatory traits, but this acclimation was insufficient to offset elevated air temperatures. Thermal tolerances acclimated to higher maximum air temperatures in two species. As a result of limited acclimation, leaf thermal safety margins (TSMs) were narrower for trees in hotter areas. We found some support for our hypothesis that species with broader thermal niches are better at acclimating to maintain more stable TSMs across the temperature gradients. These findings suggest that trees have limited abilities to acclimate to high temperatures and that thermal niche specialists may be at a heightened risk of thermal stress as global temperatures continue to rise.
Low temperature and short daylength interact to affect the leaf senescence of two temperate tree species
Authors: Wang Huanjiong , Gao Chengxi , Ge Quansheng 
Published: June 2022

Abstract
Temperature and photoperiod are two major environmental cues shaping the leaf senescence of temperate tree species. However, how the control of leaf senescence is split between photoperiod and temperature is unknown for many ecologically important species. Here, we conducted a growth chamber experiment to test the effects of temperature (6, 9, 18 and 21°C) and photoperiod (8 and 16 h daylength) on leaf senescence of two temperate tree species (Quercus mongolica Fisch. and Larix principis-rupprechtii Mayr.) distributed in montane forest of China. The results showed that low temperature (LT) alone could induce leaf senescence of both species under long daylength (LD) conditions, but the leaf senescence of L. principis-rupprechtii was more sensitive to the decrease in temperature than that of Q. mongolica under the LD condition. Short daylength (SD) alone could only induce the leaf senescence of L. principis-rupprechtii, suggesting that the photoperiod sensitivity varies between species. SD could accelerate the LT-induced senescence, but the effect of SD reduced with the decrease in temperature. Based on these findings, we developed a new autumn phenology model by incorporating interspecific differences in the photoperiod sensitivity of leaf senescence. Compared with the three existing process-based autumn phenology models, the new model was more robust in simulating the experimental data. When employing these models to available long-term phenological data, our new model also performed best in reproducing the observed leaf senescence date of two closely related species (Quercus robur L. and Larix decidua Mill.). These results enhance our understanding of how LT and SD control leaf senescence. The prediction of the climate change impacts on forest carbon uptake could be improved by incorporating this new autumn phenological model into the terrestrial biosphere models.
Coexisting Iron Deficiency Anemia and Thalassemia Traits in Infants: Implication for an Anemia Screening Program
Authors: Boonrusmee Sasivara , Thongkhao Arunwan , Wongchanchailert Malai , Mo-Suwan Ladda , Sangsupawanich Pasuree 
Published: June 2022

Abstract

Objectives
To study the prevalence of anemia among healthy infants, and outcomes of giving a therapeutic trial of iron to anemic infants in thalassemia-endemic area.

Methods
A cross sectional study was conducted in 6–9-month-old, full-term healthy infants who attended the well child clinics at 2 tertiary care centers in southern Thailand. Complete blood count and serum ferritin were performed in every infant, and hemoglobin typing was performed only in anemic cases. All anemic infants were given a therapeutic trial of iron and categorized into either; iron responder (hemoglobin increased ≥ 1 g/dL) or iron non-responder (hemoglobin increased &lt;1 g/dL) groups after one month of the therapeutic trial. Mean levels of hematological parameters, including the Mentzer index, were compared within the groups.

Results
A total of 620 infants were included in the study. From this, 230 infants (37%) were anemic for which iron deficiency contributed for 80% of the etiology. The iron responder group showed significant improvement in hematological parameters after a trial of iron, while there was no improvement in the iron non-responder group. Among iron responders, there were 31 out of 186 infants (16.6%) who had coexisting abnormal hemoglobin typing, and their post-treatment complete blood count still showed a mean corpuscular volume &lt; 70, with a Mentzer index &lt; 13.

Conclusion
Iron deficiency remains a major cause of anemia among infants, and a therapeutic trial of iron is beneficial in this age group, even though thalassemia trait/hemoglobinopathy can co-exist.