bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Malnutrition and Inflammatory Status in Cervical Cancer Patients attending Tikur Anbessa Specialized Hospital
Authors: Assefa Etsegenet , Degef Maria , Tigeneh Wondemagegnhu , Gnanasekaran Ñatesan , Legesse Mezegebu , Lejisa Tadesse 
Published: July 2022

Abstract
Gynecological cancer patients experience malnutrition and Inflammation that play a pivotal role in the progression of cervical cancer. The aim of the present study was to assess malnutrition and inflammatory status in cervical cancer patients through measurement of biochemical markers. Hospital based comparative cross-sectional study was conducted on 50 cervical cancer patients and 50 healthy individuals. Blood was collected and analyzed to gather biochemical and hematological data. Demographic and anthropometric data were also collected and data were statistically analyzed.The mean age was participants 52.4 years. Patients had decreased Albumin, Hemoglobin and Lymphocyte to Monocyte ratio and increased Total Protein, Ferritin, Red cell distribution width (RDW), Neutrophils to Lymphocytes ratio and Platelet to Lymphocytes ratio levels than controls group and also those parameters showed in cervical cancer from stage II to stage IV. Albumin negatively correlated with serum ferritin (r=-0.120*, p=0.002) and RDW (r=-0.018*, p= 0.001) in cervical cancer patient and negatively correlated with serum total protein (r=0.943*, P<0.001) in control group. NLR positively correlated with PLR (r=0.764**, p=0.000) and LMR (R=1.000**, P=0.000) in cervical cancer patients.Albumin, Total Protein and Ferritin also Hemoglobin, NLR, RDW LMR and PLR may serve as markers for assessment of malnutrition and inflammation and may also use as a prognostic factor in cervical cancer patients.
Applying genomic approaches to delineate conservation strategies: a case study using the freshwater mussel Margaritifera margaritifera in the Iberian Peninsula
Authors: Perea Silvia , Mendes Sofia , Sousa-Santos Carla , Ondina Paz , Amaro Rafaela , Castro Jaime , San-Miguel Eduardo , Lima Cristina , Garcia Maria , Velasquez Victor , Garcia-Roves Pedro , Fernández Diego , Araujo Rafael , Sousa Vitor , Reis Joaquim 
Published: July 2022

Abstract
Effective conservation actions to counteract the current decline of populations and species require a deep knowledge on their genetic structure. We used Single Nucleotide Polymorphisms (SNPs) to infer the population structure of the highly threatened freshwater pearl mussel Margaritifera margaritifera in the Iberian Peninsula. A total of 130 individuals were collected from 26 locations belonging to 16 basins. We obtained 31,692 SNPs through Genotyping by Sequencing (GBS) and used this dataset to infer population structure. Genetic diversity given as observed heterozygosity was low. Pairwise FST comparisons revealed low levels of genetic differentiation among geographically close populations. Up to 3 major genetic lineages were determined: Atlantic, Cantabrian and Douro. This structure suggests a close co-evolutionary process with brown trout (Salmo trutta), the primordial fish host of this mussel in the studied area. Some sub-basins showed some genetic structuring, whereas in others no intrapopulation differentiation was found. Our results show that genetic conservation units do not match individual basins, and that knowledge about the genetic structure is necessary before planning recovery plans that may involve relocation or restocking. The same reasoning should be applied to strictly freshwater species that are sessile or have restricted dispersal abilities and are currently imperiled worldwide.
Temporal Trend Analysis of Climate Data by using Innovative Polygon Trend Analysis method for Hardwar, India
Authors: Sharma Vishal , Ghosh Sanjay Kumar 
Published: July 2022

Abstract
Nowadays, Global warming is one of the important concerns due to which rapid change is occurring in climate throughout the world. This climate change has remarkable effects on living beings as well as on biotic and non-biotic. The climatic parameters, create a never-ending urge for the robust investigation to counter the unwanted harsh effects of change in the climate. As per the past studies, the trend analysis method is considered one of the most common and effective methods. Innovative Polygon Trend Analysis (IPTA) is an innovative, non-parametric technique of analysis for estimating trends in recent years. It almost avoids the difficulties faced in the parametric approach. The unique feature of this method is its dependence on time-series data and correspondingly comparison of data series with each other. Therefore, in this article, the average temperature (monthly) and total rainfall data (monthly) of the district of Hardwar is fetched and analyzed for the period-interval of 40 years (1981 to 2020). The result obtained consist of Polygon graphics (arithmetic and standard deviation) along with trend slope and trend length. According to the results, it is observed that no regular polygon is seen in the graphics of minimum temperature and rainfall data while regular polygon is seen in arithmetic mean of maximum rainfall. This means that minimum temperature and rainfall data varies by years and maximum temperature data has homogenous behavior. Inferring this, reciprocated incremental and decrement trends were observed in some months when the statistical parameters standard deviation and arithmetic mean are analyzed.
Focal venous malformations and risk of muscle involvement: A retrospective cohort study
Authors: Zhou Jie , Guo Lei , Song Dan , Wang Liang , Li Jing , Liu Zhuang , Niu Yanli , Sun Jiali , Wu Changhua 
Published: July 2022

Abstract
Background: Venous malformations (VMs) are the most common vascular malformations. VMs can be classified as focal, multifocal, or diffuse types. Focal venous malformations with muscle involvement is not well defined.Objective: The aim of our study was to characterize characteristics of focal VMs and classify them based on position that are associated with invasion of muscle.Methods: This is a single-center, retrospective review of patients treated for focal VMs between February 2021 and February 2022.Results: We assessed 26 patients focal VMs with 47 lesions; 18 (69%) were unifocal, 3 (12%) were dual-focal and 5（19%）were multifocal type VMs, and 29（62%) were intramuscular VMs. Of all focal VMs, 32 (68%) lesions arose in the extremities, 10 (21%) in the head and neck region and 5 (11%) in the torso. The three groups were no statistical difference for the intramuscular VMs, 19 (68%) arose in extremities, 6 (21%) in the head and neck region and 3 (11%) in the torso. But the lower limbs intramuscular VMs had a signifificantly elevated risk of focal VMs(relative risk [RR],1.7; 95% confidence interval [CI], 1.148-2.394). Limitations: Retrospective, single-institution study.Conclusion: Intramuscular involvement of the body should be considered in focal VMs. The lower limbs intramuscular VMs had a signifificantly elevated risk of focal VMs.
Ingestion of whey protein and beta-conglycinin exerts opposite effects on intestinal FGF15-mediated hepatic bile acid synthesis in mice.
Authors: Nonogaki Katsunori , Kaji Takao 
Published: July 2022

Abstract
Farnesoid X receptor (FXR), a BA receptor, and fibroblast growth factor 15 (FGF15) regulate enterohepatic circulation of BAs, glucose, and lipid metabolism. Here we show that ingestion of whey protein isolate, a milk protein, significantly increased plasma FGF15 levels and the expression of FXR and FGF15 in mouse ileum. In addition, ingestion of whey protein isolate significantly suppressed the expression of hepatic cholesterol-7α hydroxylase (CYP7A1), which induces BA synthesis, and decreased the primary BAs, including cholic acid, taurocholic acid, glycocholic acid, and taurochenodeoxycholic acid in the liver compared with controls. Moreover, ingestion of whey protein isolate significantly decreased the expression of Takeda G protein-coupled Receptor 5 (TGR5), glucagon-like peptide 1 (GLP-1), and tryptophan hydroxylase1 (Tph1) in the small intestine, and plasma serotonin and insulin levels. On the other hand, ingestion of the soy protein β-conglycinin significantly decreased the expression of FGF15 in the ileum and plasma FGF15 levels, leading to increased expression of hepatic CYP7A1 and BA synthesis. Moreover, ingestion of β-conglycinin significantly increased the expression of intestinal TGR5, GLP-1, and Tph1, and plasma 5-HT and insulin levels. These findings suggest that whey protein and β-conglycinin have opposite effects on intestinal FGF15-mediated hepatic BA synthesis and intestinal serotonin secretion in mice.
The Beneficiary of bone marrow-derived mesenchymal stem cells primed with oestradiol in alleviating collagen-induced arthritis​
Authors: Jahantigh Monireh , Froushani Seyyed Meysam Abtahi , Ahangaran Nahideh Afzale 
Published: July 2022

Abstract
This study was conducted to investigate the effects of the oestradiol (ES)pulsed bone marrow-derived mesenchymal stem cell (BM-MSC) to treat adjuvant-induced arthritis in Wistar rats. BM-MSCs were pulsed with ES (0, 10,100 and 1000 nM) for 24 hours. RA induced collagen and Freund's Complete Adjuvant into the base of the tail of Wistar rats. In vitro results showed that the least effective concentration of ES that can promote potent anti-inflammatory properties in the MSC population is 100 nM. At this concentration, ES increases the inhibition of the poly-clonal T lymphocyte proliferation, production of IDO, IL-10, Nitric oxide, TGF-β, and expression of CXCR4 and CCR2 mRNA in the MSC population. Based on In vitro results, the RA rats were treated with 2×106 MSCs or ES-pulsed MSCs (100nM) on day 10 when all animals had developed signs of RA. The in vivo results showed that the treatment with ES-pulsed BM-MSCs reduced the severity of the RA more profoundly than treatment with BM-MScs alone. The ability of ES-pulsed BM-MSCs to reduce symptoms and RA markers like CRP, RF, and nitric oxide was comparable to that of prednisolone. Prednisolone was more successful in reducing inflammatory cytokines than treatment with ES-pulsed BM-MSCs. ES-pulsed BM-MSCs were more successful in increasing anti-inflammatory cytokines than treatment with Prednisolone. The ability of ES-pulsed BM-MSCs to decrease the level of nitric oxide was comparable to that of prednisolone. Collectively, ES-pulsed BM-MSCs may be a helpful strategy in controlling RA.
Single-cell Landscape Reveals the Immune Heterogeneity of Bone Marrow Involvement in Peripheral T-cell lymphoma
Authors: Liu Jun , Xia Baijing , Jiang Xinmiao , Cao Lixue , Xi Zhihui , Liang Liting , Zhang Shaojun , Zhang Hui , Li Wenyu 
Published: July 2022

Abstract
Evaluation of Bone marrow involvement (BMI) in Peripheral T-cell lymphoma (PTCL) patients is quite important for determining the prognosis and the tumor microenvironment of BM in PTCL remains unclear. We collected 11 fresh BM samples from the patients with BMI and performed the single-cell RNA-seq (scRNA-seq) analysis to reveal the immune landscape and genetic variation of different prognosis of PTCL. There were more effector T cells, exhausted lymphocyte cells and inflammatory response in AITL compare with PTCL-NOS. The immune heterogeneity in AITL was associated with prognosis. More specific TCR-T cells enriched was observed in patients with better response to anti-CD30 therapy. RHOA mutation associated neoantigen was observed. In those treated with Chidamide, more CD4 + regulate cells resulted in better response. In the progressive group, T cell enrichment progressed to secondary B cells enrichment was found and later developed diffuse large B cell lymphoma. Moreover, the AITL patient with lymphoma-associated hemophagocytic syndrome contained the precursor exhausted cells with the copy number variation in Chr5. Overall, this study revealed the first single cell landscape depicting BM microenvironment heterogeneity in PTCL with BMI. It is innovative in applying the scRNA-seq technology to investigate the immune heterogeneity and the genetic variation of AITL.
Opinions of Turkish medical oncologists about the participation of patients and/or patient caregivers in multidisciplinary tumor boards
Authors: Eren Orhan Onder , Zerey Mehmet Murat , Körez Muslu Kazım , Ata Ozlem 
Published: July 2022

Abstract
Purpose
Multidicplinary tumor board(MTBs) are an an important component of cancer patient care. The participation of patients or caregivers in MTBs is an contentious issue and is uncommon in Turkey. This study aimed to determine what Turkish medical oncologists think about the participation of patients and caregivers in MTBs.
Method
The study was conducted during 8th Turkish Congress Of Medical Oncology in Antalya İn November 2021. The medical oncologists that participated completed a 9-item questionnaire. The relationships between the participants’ sociodemographic characteristics and their questionnaire responses concerning MTBs were analyzed using Pearson’s chi-square test or the Fisher-Freeman-Halton test.
Results
No statistically significant correlation was found between the socio-demographic characteristics of the participants and the opinions of cancer patients or their relatives about their participation in multidisciplinary tumor boards. 75% of the participants do not approve participation of patient or caregivers in MTBs. 12% of participants approve the participation of both in the MTBs. 3% of participants approve only the participation of caregivers where as only the 6% of medical oncologist approve only the participation of patients in tumor board.
Conclusion
Among 109 Turkish medical oncologists, most didn’t approve of the participation of patients or caregivers in MTBs. The 2 major reasons for this lack of approval are fear that patients and caregivers will not understand medical terminology, and the emotional stress their participation can cause MTB members.
Working with a medical quality register increases nurses’ clinical and organisational competence – a qualitative study
Authors: Bjørngaard Hilde , Koksvik Hege Svean , Jakobsen Bente , Grønning Kjersti 
Published: July 2022

Abstract
Background
RevNatus is a consent-based, nationwide medical quality register that collects data on patients with inflammatory rheumatic diseases during pregnancy and one year postpartum. The entering of data takes place in outpatient clinics in rheumatology wards in hospitals. The aim of this study is to explore how rheumatology nurses experience organizing and working with the medical quality register RevNatus in addition to their normal clinical patient-care tasks.
Methods
Qualitative focus group interviews and individual in-depth interviews were conducted to gain insights into how nurses organize performing quality register work and clinical work simultaneously. Data were analysed using systematic text condensation.
Results
The informants represented seven different rheumatology outpatient clinics in Norway. The analyses showed that working with RevNatus increased the nurses’ knowledge about pregnancy and rheumatic diseases, improved the content of their nurse consultations and found the ‘register form’ as a useful template to structure the nurse consultations. The nurses took the main responsibility for RevNatus, but lack of routines and uncoordinated collaboration with the rheumatologists and secretaries made the nurses spend too much time verifying the accuracy of data or post-registering missing data.
Conclusion
The nurses experienced work with RevNatus as time-consuming, but the register work increased both their clinical and organisational competences. Routines and collaboration within the registry team are important to ensure the data quality and reduce the workload.
Characterization of a new anti-LAG3 monoclonal for immuno-oncology
Authors: Warren P. Daniel , Dodson Mark S. , Smith Margaret H. , Landowski Terry H. , Palting John Douglas , Towne Penny 
Published: July 2022

Abstract
Background
Lymphocyte activation gene 3 (LAG3) (CD223) is a T cell inhibitory receptor that promotes tumor cell immune escape, is associated with signaling pathways in multiple immune disorders and cancer, and is considered as a potential target for cancer diagnostic and immunotherapeutic applications. Targeting LAG3 towards these ends requires developing additional new antibodies. Here we compare the binding characteristics of a new anti-LAG3 rabbit antibody, the SP464 clone, with the thirty-year old and extensively used anti-LAG3 mouse antibody, the 17B4 clone.
Methods
The three orthologous techniques of automated capillary electrophoresis (ACE), surface plasmon resonance (SPR), and immunohistochemistry (IHC) were used to assess the relative binding properties of each clone for their shared immunogen and its sub-fragments and to map the relative location of their respective epitopes within the immunogen. Alanine scanning substitution of the SP464 epitope was used to further refine the epitope boundaries and to identify amino acids essential for binding.
Results
The rabbit SP464 clone exhibited between 20x to 30x greater binding to the target than did the mouse 17B4 clone. The minimal epitopes of the SP464 and 17B4 clones were localized to separate, but overlapping, sub-fragments within the amino-terminal fifteen acids of the original thirty-mer peptide immunogen used to generate both antibodies. Further application of this orthologous approach towards quantifying the effects of sequential alanine substitutions along the minimal epitope of the rabbit SP464 clone were in close agreement and identified two amino acids within the SP464 epitope that are essential for binding and four additional amino acids that likely contribute towards binding.
Conclusions
Our results show that the combined application of ACE, SPR, and IHC constitute a powerful orthologous approach both for comparing antibody-binding characteristics and for fine mapping of linear epitopes within an immunogen. The results of this approach indicate that the rabbit clone SP464 may serve as an additional useful antibody for assessing LAG3 expression.
The morphological characteristics of paraspinal muscles in young patients with unilateral neurological symptoms of lumbar disc herniation.
Authors: Zhao Xuan , Liang Huiqiang , Hua Zijian , Li Wenshuai , Li Jia , Wang Linfeng , Shen Yong 
Published: July 2022

Abstract
Objective:
The objective of this study was to explore the morphological characteristics of paraspinal muscles in young patients with unilateral neurological symptoms of lumbar disc herniation.
Methods:
This study retrospectively analyzed young patients aged 18–40 years who were hospitalized for lumbar disc herniation in our hospital from June 2017 to June 2020. The data of gender, age, body mass index (BMI), duration of symptoms, degree of lumbar disc herniation, (Visual Analogue Scale) VAS score for LBP, relative cross-sectional area (RCAS) of paravertebral muscles (psoas major, PM, multifidus, MF and erector spinae, ES), and degree of fat infiltration (DFF) of paravertebral muscles were collected. According to whether the VAS score > 4, patients were divided into low back pain (LBP) group and no-low back pain (NLBP) group. The demographic characteristics, bilateral and ipsilateral paravertebral muscles of the two groups were compared and analyzed.
Result:
A total of 129 patients were included in this study, 52 with LBP and 77 with NLBP. There were no significant differences in gender, BMI, and Pfirrmann grade of lumbar disc herniation between the two groups (P > 0.05). The duration of symptoms in the LBP group(9.15 ± 0.31 months) was longer than that in the NLBP group (3.72 ± 0.48 months)(P < 0.05), and the age (33.58 ± 2.98 years) was longer than that in the NLBP group (24.13 ± 2.15 years) (P = 0.002).At L3/4 and L5/S1,there was no significant difference in RCSA and DFF of bilateral and ipsilateral paraspinal muscles between the LBP group and the NLBP group. At L4/5, there was no significant difference in RCSA and DFF of the paraspinal muscles on both sides of the LBP group (P > 0.05); In the NLBP group, the RCSA of the MF muscle on the diseased side was smaller than that on the normal side (P < 0.05), and the DFF of the MF muscle on the diseased side was larger than that on the normal side(P < 0.05), there was no significant difference in the ES and PM muscle on both sides (P > 0.05).At L4/5, the RCSA of the normal side MF muscle was signifcantly smaller in the LBP group than in the NLBP group (P < 0.05), the DFF of the normal side MF muscle was signifcantly larger in the LBP group than in the NLBP group (P < 0.05). There was no significant difference in the ES and PM muscle on the same side between the two groups (P > 0.05).
Conclusion:
In young patients with unilateral neurological symptoms of lumbar disc herniation, symmetrical atrophy of the bilateral MF muscle is more prone to cause low back pain. Longer duration of symptoms, older age, and greater atrophy of the normal side MF muscle could increase the incidence of low back pain in young patients with unilateral neurological symptoms of lumbar disc herniation.
Bioinformatic analysis identifying Arylsulfatase B as a novel biomarker for cancer prognosis with a highlight on stomach adenocarcinoma
Authors: Tian Mengyue , Xue Chaorong , Zhang Shiqi , Yang Yifei , Lin Shizhao , Ao Lu , Li Suyu , Lin Xinjian 
Published: July 2022

Abstract
Arylsulfatases are lysosomal enzymes with important function in the cell metabolism. Studies have demonstrated that the lysosomal protein arylsulfatase B (ARSB) may play roles in tumorigenesis and metastatic potential of tumor cells. However, the relationship between ARSB and stomach adenocarcinoma (STAD) has not yet been established. The aim of this study was to investigate the prognostic value of ARSB in cancers, particularly STAD and explore the potential underlying mechanisms. Gene expression data and detailed clinical information of cancer cases were obtained from multiple public databases. Subsequently, a series of bioinformatics analyses were used to elucidate the effects of ARSB on malignant tumors including STAD. The results demonstrated that ARSB was up-regulated in 15 cancers including STAD, and down-regulated in 10 cancers. In most cancers, ARSB expression was significantly related with CNA, methylation levels, and overall survival time. In STAD, ARSB was positively related with ESTIMATE Score (r = 0.54, p < 0.001), Immune Score (r = 0.35, p < 0.001), Stromal Score (r = 0.65, p < 0.001), and negatively related with Tumor Purity (r = -0.54, p < 0.001), TMB value (r = -0.13, p = 0.0092) and MSI value (r = -0.16, p = 0.0015). Meanwhile, ARSB are related with 11 tumor-infiltrating immune cells and immune checkpoint genes including LAG3, TIGIT and PDCD1. TNN mutation widely occurred in both high- and low-ARSB expression group. ARSB was found to be related to a variety of drug sensitivities, including lapatinib (r = 0.19, P < 0.001), acetalax (r = 0.18, P < 0.001), osimertinib (r = 0.17, P < 0.001), ABT737 (r = 0.14, P < 0.001), afatinib (r = 0.14, P < 0.001), spitinib (r = 0.13, P < 0.001). Thus, ARSB might be a potential therapeutic target and prognostic biomarker for cancers, especially STAD.