bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineered CaM2 modulates nuclear calcium oscillation and enhances legume root nodule symbiosis
Authors: del Cerro Pablo , Cook Nicola M. , Huisman Rik , Dangeville Pierre , Grubb Lauren E. , Marchal Clemence , Ho Ching Lam Anson , Charpentier Myriam 
Published: March 2022

Significance
Oscillations in intracellular calcium concentration play an essential role in the regulation of multiple cellular processes. In plants capable of root endosymbiosis with nitrogen-fixing bacteria and/or arbuscular mycorrhizal fungi, nuclear localized calcium oscillations are essential to transduce the microbial signal. Although the ion channels required to generate the nuclear localized calcium oscillations have been identified, their mechanisms of regulation are unknown. Here, we combined proteomics and engineering approaches to demonstrate that the calcium-bound form of the calmodulin 2 (CaM2) associates with CYCLIC NUCLEOTIDE GATED CHANNEL 15 (CNGC15s), closing the channels and providing the negative feedback to sustain the oscillatory mechanism. We further unraveled that the engineered CaM2 accelerates early endosymbioses and enhanced root nodule symbiosis but not arbuscular mycorrhization.
Leisure Education, Poverty and Recreation Participation
Authors: Hiebert Chelsey , Oancescu Jacquelyn 
Published: March 2022

Abstract
Individuals living in poverty face a number of challenges that interfere with their ability to create or engage in meaningful leisure and recreation experiences. Although limited access to finances is often the most cited constraint to leisure and recreation participation among those living in poverty, there are a wide range of other constraints on participation: limited leisure skills; knowledge and interests; discriminatory policies; feelings of guilt and shame; and poor physical, social and mental wellbeing. Despite the vast array of policies, subsidized programming and supports that the recreation profession and allied professionals have made available, low-income families continue to struggle to access and create leisure experiences. This case study highlights the innovation of a community-based leisure education delivery system to help low-income families learn about leisure, develop the necessary skills, knowledge, capacities and resources, and gain access to a wide range of leisure and recreation experiences to enhance their leisure repertoire. The success of the agency is embedded in their invested stakeholders, their ability to utilize existing community leisure recreation resources, and their focus on teaching for and through leisure. Because of this agency’s unique approach, the application of leisure education at the community level has positively impacted not only the child, but the family unit and community as well.

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Information
Vancouver Island University World Leisure Centre of Excellence
© C. Hiebert and J. Oncescu 2015
Convexity of a ratio of the modified Bessel functions of the second kind with applications
Authors: Yang Zhen-Hang , Tian Jing-Feng 
Published: March 2022

LetKνK_{\nu }be the modified Bessel functions of the second kind of orderν\nu. The ratioQν(x)=xKν−1(x)/Kν(x)Q_{\nu }\left ( x\right ) =xK_{\nu -1}\left ( x\right ) /K_{\nu }\left ( x\right )appeared in physics and probability. In this paper, we collate properties of this ratio, and prove the conjecture that(−1)nQν(n)(x)>(>)0\left ( -1\right ) ^{n}Q_{\nu }^{\left ( n\right ) }\left ( x\right ) >\left ( >\right ) 0forx>0x>0andn=2,3n=2,3if|ν|>(>)1/2\left \vert \nu \right \vert >\left ( >\right ) 1/2holds forn=2n=2. This yields several new consequences and improves some known results. Finally, two conjectures are proposed.
Do Hypertensive Men Spy With an Angry Little Eye? Anger Recognition in Men With Essential Hypertension - Cross-sectional and Prospective Findings
Authors: Auer Alisa , von Känel Roland , Lang Ilona , Thomas Livia , Zuccarella-Hackl Claudia , Degroote Cathy , Gideon Angelina , Wiest Roland , Wirtz Petra H 
Published: March 2022

Abstract

Background
Higher trait anger has inconsistently been associated with hypertension and hypertension development, but social context in terms of recognition of other persons’ anger has been neglected in this context.

Purpose
Here, we investigated anger recognition of facial affect and trait anger in essential hypertensive and normotensive men in addition to prospective associations with blood pressure (BP) increases.

Methods
Baseline assessment comprised a total of 145 participants including 57 essential hypertensive and 65 normotensive men who were otherwise healthy and medication-free. Seventy-two eligible participants additionally completed follow-up assessment 3.1 (±0.08 SEM) years later to analyze BP changes over time. We assessed emotion recognition of facial affect with a paradigm displaying mixed facial affect of two morphed basic emotions including anger, fear, sadness, and happiness. Trait anger was assessed with the Spielberger trait anger scale.

Results
Cross-sectionally, we found that with increasing BP, hypertensive men overrated anger displayed in facial expressions of mixed emotions as compared to normotensive men (ps ≤ .019) while there were no differences in trait anger (p = .16). Prospectively, the interaction between mean anger recognition and trait anger independently predicted BP increases from baseline to follow-up (ps ≤ .043), in that overrating displayed anger predicted future BP increases only if trait anger was high.

Conclusions
Our findings indicate an anger recognition bias in men with essential hypertension and that overrating displayed anger in combination with higher trait anger seems to predict future BP increases. This might be of clinical relevance for the development and progression of hypertension and cardiovascular disease.
Increased resolution in the face of conflict: phylogenomics of the Neotropical bellflowers (Campanulaceae: Lobelioideae), a rapid plant radiation
Authors: Lagomarsino Laura P , Frankel Lauren , Uribe-Convers Simon , Antonelli Alexandre , Muchhala Nathan 
Published: March 2022

Abstract

Background and Aims
The centropogonid clade (Lobelioideae: Campanulaceae) is an Andean-centred rapid radiation characterized by repeated convergent evolution of morphological traits, including fruit type and pollination syndromes. While previous studies have resolved relationships of lineages with fleshy fruits into subclades, relationships among capsular species remain unresolved. This lack of resolution has impeded reclassification of non-monophyletic genera, whose current taxonomy relies heavily on traits that have undergone convergent evolution.

Methods
Targeted sequence capture using a probe-set recently developed for the centropogonid clade was used to obtain phylogenomic data from DNA extracted from both silica-dried and herbarium leaf tissue. These data were used to infer relationships among species using concatenated and partitioned species tree methods, and to quantify gene tree discordance.

Key Results
While silica-dried leaf tissue resulted in longer assembled sequence data, the inclusion of herbarium samples improved taxonomic representation. Relationships among baccate lineages are similar to those inferred in previous studies, although they differ for lineages within and among capsular clades. We improve the phylogenetic resolution of Siphocampylus, which forms ten groups of closely related species which we informally name. Two subclades of Siphocampylus and two individual species are rogue taxa whose placement differs widely across analyses. Gene tree discordance (including cytonuclear discordance) is rampant.

Conclusions
This first phylogenomic study of the centropogonid clade considerably improves our understanding of relationships in this rapid radiation. Differences across analyses and the possibility of additional lineage discoveries still hamper a solid and stable reclassification. Rapid morphological innovation corresponds with a high degree of phylogenomic complexity, including cytonuclear discordance, nuclear gene tree conflict and well-supported differences between analyses based on different nuclear loci. Together, these results point to a potential role of hemiplasy underlying repeated convergent evolution. This hallmark of rapid radiations is probably present in many other species-rich Andean plant radiations.
Dorsal Preservation Surgery: A Novel Modification for Dorsal Shaping and Hump Reduction
Authors: Tuncel Umut , Kurt Alper , Saban Yves 
Published: March 2022

AbstractBackgroundA new technique for dorsal preservation rhinoplasty that minimizes recurrent hump deformity is introduced.ObjectivesThe authors sought to present a novel dorsal roof flap (DRF) technique for modifying the nasal hump and dorsum while reducing morbidity.MethodsTwenty-two primary and 3 secondary rhinoplasty patients had 1 of 2 types of nasal DRF depending on hump composition. A triangular roof flap was created utilizing 2 incisions from W-point to lateral Keystone junction in cartilaginous humps (cartilaginous DRF) and to the nasion in osseocartilaginous humps (osseocartilaginous DRF). Then, septal strip excisions were conducted to lower the profile followed by nasal base narrowing. The DRF was then lowered to the desired profile line, any excess lateral wall height trimmed, and the DRF sutured to the upper lateral cartilages. Preoperative and postoperative nasolabial and nasoglabellar angles were measured on the photos.ResultsMean follow-up was 10.3 (standard deviation, 4.41) months (range, 6-20 months). A total 22 humps were V-shape and 3 were S-shape. The composition of the hump was cartilaginous in 5 cases, bone in 7 cases, and combined in 13 cases. A cartilaginous DRF was utilized for the 5 cases with a cartilaginous hump and osseocartilaginous DRF was employed in the other 20 cases. There were no complications observed in the follow-up period and thus no revisions.ConclusionsThe DRF technique allows preservation of the nasal dorsum and elimination of the dorsal hump while minimizing hump recurrence.Level of Evidence: 4
RabbitV: fast detection of viruses and microorganisms in sequencing data on multi-core architectures
Authors: Zhang Hao , Chang Qixin , Yin Zekun , Xu Xiaoming , Wei Yanjie , Schmidt Bertil , Liu Weiguo 
Published: March 2022

Abstract

Motivation
Detection and identification of viruses and microorganisms in sequencing data plays an important role in pathogen diagnosis and research. However, existing tools for this problem often suffer from high runtimes and memory consumption.

Results
We present RabbitV, a tool for rapid detection of viruses and microorganisms in Illumina sequencing datasets based on fast identification of unique k-mers. It can exploit the power of modern multi-core CPUs by using multi-threading, vectorization and fast data parsing. Experiments show that RabbitV outperforms fastv by a factor of at least 42.5 and 14.4 in unique k-mer generation (RabbitUniq) and pathogen identification (RabbitV), respectively. Furthermore, RabbitV is able to detect COVID-19 from 40 samples of sequencing data (255 GB in FASTQ format) in only 320 s.

Availability and implementation
RabbitUniq and RabbitV are available at https://github.com/RabbitBio/RabbitUniq and https://github.com/RabbitBio/RabbitV.

Supplementary information
Supplementary data are available at Bioinformatics online.
S100B inhibition protects from chronic experimental autoimmune encephalomyelitis
Authors: Barros Catarina , Barateiro Andreia , Neto Alexandre , Soromenho Beatriz , Basto Afonso P. , Mateus Joana M. , Xapelli Sara , Sebastião Ana M. , Brites Dora , Graça Luís , Fernandes Adelaide 
Published: March 2022

Abstract
Studies have correlated excessive S100B, a small inflammatory molecule, with demyelination and associated inflammatory processes occurring in multiple sclerosis. The relevance of S100B in multiple sclerosis pathology brought an emerging curiosity highlighting its use as a potential therapeutic target to reduce damage during the multiple sclerosis course, namely during inflammatory relapses. We examined the relevance of S100B and further investigated the potential of S100B-neutralizing small-molecule pentamidine in chronic experimental autoimmune encephalomyelitis. S100B depletion had beneficial pathological outcomes and based on promising results of a variety of S100B blockade strategies in an ex vivo demyelinating model, we choose pentamidine to assay its role in the in vivo experimental autoimmune encephalomyelitis. We report that pentamidine prevents more aggressive clinical symptoms and improves recovery of chronic experimental autoimmune encephalomyelitis. Blockade of S100B by pentamidine protects against oligodendrogenesis impairment and neuroinflammation by reducing astrocyte reactivity and microglia pro-inflammatory phenotype. Pentamidine also increased regulatory T cell density in the spinal cord suggesting an additional immunomodulatory action. These results showed the relevance of S100B as a main driver of neuroinflammation in experimental autoimmune encephalomyelitis and identified an uncharacterized mode of action of pentamidine, strengthening the possibility to use this drug as an anti-inflammatory and remyelinating therapy for progressive multiple sclerosis.
MRI tracking of human Wharton’s jelly stem cells seeded onto acellular dermal matrix labeled with superparamagnetic iron oxide nanoparticles in burn wounds
Authors: Mehrabani Davood , Nazempour Mehra , Mehdinavaz-Aghdam Rouhollah , Hashemi Seyedeh-Sara , Jalli Reza , Moghadam Mahdi Saeedi , Zare Shahrokh , Jamhiri Iman , Moayedi Javad , Karimi-Busheri Feridoun 
Published: March 2022

Abstract

Background
In vivo cell tracking after transplantation in regenerative medicine remains an unmet challenge and limits current understanding of the wound healing mechanism through cell-based therapies. This study investigated tracking of human Wharton’s jelly stem cells (hWJSCs) seeded onto an acellular dermal matrix (ADM) and labeled with superparamagnetic iron oxide nanoparticles (SPIONs) by magnetic resonance imaging (MRI) in burn injury.

Method
The hWJSCs were characterized and assessed for growth kinetics. A total of 30 rats were enrolled in three equal groups. Group 1 underwent scald burn injury left without treatment, the group 2 was treated by an ADM that was prepared from cosmetic surgery skin samples and the group 3 received hWJSCs labeled with SPIONs seeded onto an ADM. Tensile strength was evaluated before and after interventions, real time PCR assessed apoptosis, and Prussian blue staining, scanning electron microscopy (SEM) and MRI were used for the tracking of labeled cells.

Results
The hWJSCs exhibited mesenchymal stem cell properties. Population doubling time was 40.1 hours. SPIONs did not show any toxic effect. The hWJSCs seeded onto an ADM decreased Bax and increased Bcl-2 gene expression. Internalization of SPIONs within hWJSCs was confirmed by Prussian blue staining, SEM and MRI until day 21. There was a significant difference between the Young’s moduli of normal skin and the group receiving hWJSCs seeded onto an ADM. Histological observations and SEM imaging confirmed that MRI is an accurate method to track SPION-labeled hWJSCs in vivo.

Conclusions
This study showed that SPION labeling coupled with MRI can be used to further understand the fate of stem cells after transplantation in a burn model.
Large pulmonary artery pseudoaneurysm after CardioMEMS implantation: a case report
Authors: Garg Tushar , Raikhelkar Jayant , Gilkeson Robert , Tavri Sidhartha 
Published: March 2022

Abstract

Background
CardioMEMS heart failure (HF) system is an implantable wireless pressure sensor that is placed in a branch of the pulmonary artery (PA) for remote monitoring of PA pressures in patients with HF. Pulmonary artery injury/haemoptysis can occur during the sensor placement.

Case summary
An 80-year-old male patient with HF with reduced ejection fraction (20%) underwent CardioMEMS HF system implantation for recurrent shortness of breath. He developed haemoptysis and dyspnoea during the procedure, which was managed with furosemide. The patient’s computerized tomographic angiography showed a 3.4 cm pseudoaneurysm with active extravasation from the superior segmental branch of the left PA due to injury during device placement. The decision to embolize the pseudoaneurysm was made after a multi-disciplinary team meeting and discussion with the patient. The embolization procedure was carried out successfully with the final left pulmonary angiogram showed complete stasis and no further filling of the pseudoaneurysm sac.

Discussion
The incidence of mortality in patients with PA injury from CardioMEMS devices is high, and therefore prompt diagnosis and management are critical. Pulmonary artery pseudoaneurysms are uncommon and present with haemoptysis. Transcatheter embolization has been shown to be a practical, effective, and safe therapeutic option in stable patients.
A case report of a long-term survivor after inadvertent ligation of the left pulmonary artery during intended ductal ligation
Authors: Zeng Qi-xian , Liu Zhihong , Xi Qun-Ying , Xiong Changming 
Published: March 2022

Abstract

Background
Patent ductus arteriosus (PDA) can close on its own during childhood. Patent ductus arteriosus with left pulmonary artery (LPA) occlusion is rare. Here, we describe possible aetiologies of this condition and treatment strategies based on recent guidelines.

Case summary
A 35-year-old man experienced shortness of breath for 20 years. Physical examination revealed pitting oedema, digital clubbing, and bi-phasic murmur along the left sternal border at the 2nd and 3rd intercostal space. Congenital heart disease was suspected. Echocardiography revealed a severely dilated pulmonary trunk and PDA; however, the LPA was not visible. The patient has undergone PDA ligation surgery &gt;30 years ago, which may have caused accidental LPA ligation; however, extreme elevation of pulmonary pressure led to increased difficulties in performing LPA reconstruction and PDA division. Therefore, pulmonary arterial hypertension (PAH) initial combination therapy with parenteral prostanoids was prescribed. The patient’s condition improved gradually.

Discussion
Inadvertent ligation of the LPA instead of PDA is a rare and usually fatal complication during ductal ligation. Patients who survive this catastrophic complication usually develop the progressive pulmonary vascular disease with increased pulmonary pressure and impaired lung parenchyma resulting in right heart and respiratory failure. Early and regular follow-up with cardiac imaging studies is important to diagnose this complication. Reconstruction of the ligated LPA and PDA late in the disease course is difficult due to the development of pulmonary arterial hypertension. Initial PAH combination therapy may be valuable for relieving the patients’ symptoms at that stage. Heart and lung transplantation can also be considered in suitable patients.
A case report of a novel mutation in lamin A/C gene related with risk of sudden death
Authors: Sanchez Vallejo Carlos Andres , Rodriguez Ariza Carlos Daniel , Restrepo Urbina Jose Alfredo , Callegari Osorio Santiago 
Published: March 2022

Abstract

Background
Lamin A/C-associated heart disease is a group of clinical entities characterized by a mutation in the LMNA gene. Multiple cardiac phenotypes have been described, including a higher risk of sudden death.

Case summary
A 23-year-old asymptomatic patient with an extensive history of heart disease in the family consulted the clinic. He had a genetic test performed when he was born revealing a new frameshift mutation in the LMNA gene. Numerous cardiac function tests were ordered, which initially were normal. After a year of follow-up, Holter monitoring was positive for episodes of nonsustained ventricular tachycardia (NSVT). Because of the risk factors and higher likelihood of sudden death, a decision was made to offer an implantable cardiac defibrillator (ICD), which was performed without complications. The patient continues the follow-up with cardiology and electrophysiology consisting of yearly cardiac imaging and device recordings.

Discussion
Lamins are nuclear proteins involved in various cellular processes in myocardial cells. Therefore, mutations are associated with wide phenotypic alterations. The mutation described here was not previously reported in the literature. In the face of an undescribed mutation, the decision to use an ICD for primary prevention of sudden death is challenging. Because of the episodes of NSVT and a higher likelihood of risk of sudden death due to male sex and first-degree atrioventricular block, the decision to use an ICD was made for this patient, with no complications.
Divergent regulatory roles of NuRD chromatin remodeling complex subunits GATAD2 and CHD4 in Caenorhabditis elegans
Authors: Golden Nicole L , Foley Michaela K , Kim Guisbert Karen S , Guisbert Eric 
Published: March 2022

Abstract
During proteotoxic stress, a pathway known as the heat shock response is induced to maintain protein-folding homeostasis or proteostasis. Previously, we identified the Caenorhabditis elegans GATAD2 ortholog, dcp-66, as a novel regulator of the heat shock response. Here, we extend these findings to show that dcp-66 positively regulates the heat shock response at the cellular, molecular, and organismal levels. As GATAD2 is a subunit of the nucleosome remodeling and deacetylase chromatin remodeling complex, we examined other nucleosome remodeling and deacetylase subunits and found that the let-418 (CHD4) nucleosome repositioning core also regulates the heat shock response. However, let-418 acts as a negative regulator of the heat shock response, in contrast to positive regulation by dcp-66. The divergent effects of these two nucleosome remodeling and deacetylase subunits extend to the regulation of other stress responses including oxidative, genotoxic, and endoplasmic reticulum stress. Furthermore, a transcriptomic approach reveals additional divergently regulated pathways, including innate immunity and embryogenesis. Taken together, this work establishes new insights into the role of nucleosome remodeling and deacetylase subunits in organismal physiology. We incorporate these findings into a molecular model whereby different mechanisms of recruitment to promoters can result in the divergent effects of nucleosome remodeling and deacetylase subunits.