bluuebunny/crossref_metadata_2025_split huggingface dataset:
Convergent star products on cotangent bundles of Lie groups
Authors: Heins Michael , Roth Oliver , Waldmann Stefan 
Published: April 2022

AbstractFor a connected real Lie group G we consider the canonical standard-ordered star product arising from the canonical global symbol calculus based on the half-commutator connection of G. This star product trivially converges on polynomial functions on $$T^*G$$

T
∗

G

thanks to its homogeneity. We define a nuclear Fréchet algebra of certain analytic functions on $$T^*G$$

T
∗

G

, for which the standard-ordered star product is shown to be a well-defined continuous multiplication, depending holomorphically on the deformation parameter $$\hbar $$
ħ
. This nuclear Fréchet algebra is realized as the completed (projective) tensor product of a nuclear Fréchet algebra of entire functions on G with an appropriate nuclear Fréchet algebra of functions on $${\mathfrak {g}}^*$$

g

∗

. The passage to the Weyl-ordered star product, i.e. the Gutt star product on $$T^*G$$

T
∗

G

, is shown to preserve this function space, yielding the continuity of the Gutt star product with holomorphic dependence on $$\hbar $$
ħ
.
Impact of a Microbial Pest Control Product Containing Bacillus thuringiensis on Brood Development and Gut Microbiota of Apis mellifera Worker Honey Bees
Authors: Steinigeweg Charlotte , Alkassab Abdulrahim T. , Erler Silvio , Beims Hannes , Wirtz Ina P. , Richter Dania , Pistorius Jens 
Published: April 2022

AbstractTo avoid potential adverse side effects of chemical plant protection products, microbial pest control products (MPCP) are commonly applied as biological alternatives. This study aimed to evaluate the biosafety of a MPCP with the active organism Bacillus thuringiensis ssp. aizawai (strain: ABTS-1857). An in-hive feeding experiment was performed under field-realistic conditions to examine the effect of B. thuringiensis (B. t.) on brood development and the bacterial abundance of the core gut microbiome (Bifidobacterium asteroids, Gilliamella apicola, the group of Lactobacillus and Snodgrasella alvi) in Apis mellifera worker bees. We detected a higher brood termination rate and a non-successful development into worker bees of treated colonies compared to those of the controls. For the gut microbiome, all tested core members showed a significantly lower normalized abundance in bees of the treated colonies than in those of the controls; thus, a general response of the gut microbiome may be assumed. Consequently, colony exposure to B. t. strain ABTS-1857 had a negative effect on brood development under field-realistic conditions and caused dysbiosis of the gut microbiome. Further studies with B. t.–based products, after field-realistic application in bee attractive crops, are needed to evaluate the potential risk of these MPCPs on honey bees.
Intraoperative MET-receptor targeted fluorescent imaging and spectroscopy for lymph node detection in papillary thyroid cancer: novel diagnostic tools for more selective central lymph node compartment dissection
Authors: Jonker Pascal K. C. , Metman Madelon J. H. , Sondorp Luc H. J. , Sywak Mark S. , Gill Anthony J. , Jansen Liesbeth , Links Thera P. , van Diest Paul J. , van Ginhoven Tessa M. , Löwik Clemens W. G. M. , Nguyen Anh H. , Coppes Robert P. , Robinson Dominic J. , van Dam Gooitzen M. , van Hemel Bettien M. , Fehrmann Rudolf S. N. , Kruijff Schelto 
Published: April 2022

Abstract

Purpose
Patients undergoing prophylactic central compartment dissection (PCLND) for papillary thyroid cancer (PTC) are often overtreated. This study aimed to determine if molecular fluorescence-guided imaging (MFGI) and spectroscopy can be useful for detecting PTC nodal metastases (NM) and to identify negative central compartments intraoperatively.

Methods
We used a data-driven prioritization strategy based on transcriptomic profiles of 97 primary PTCs and 80 normal thyroid tissues (NTT) to identify tumor-specific antigens for a clinically available near-infrared fluorescent tracer. Protein expression of the top prioritized antigen was immunohistochemically validated with a tissue microarray containing primary PTC (n = 741) and NTT (n = 108). Staining intensity was correlated with 10-year locoregional recurrence-free survival (LRFS). A phase 1 study (NCT03470259) with EMI-137, targeting MET, was conducted to evaluate safety, optimal dosage for detecting PTC NM with MFGI, feasibility of NM detection with quantitative fiber-optic spectroscopy, and selective binding of EMI-137 for MET.

Results
MET was selected as the most promising antigen. A worse LRFS was observed in patients with positive versus negative MET staining (81.9% versus 93.2%; p = 0.02). In 19 patients, no adverse events related to EMI-137 occurred. 0.13 mg/kg EMI-137 was selected as optimal dosage for differentiating NM from normal lymph nodes using MFGI (p < 0.0001) and spectroscopy (p < 0.0001). MFGI identified 5/19 levels (26.3%) without NM. EMI-137 binds selectively to MET.

Conclusion
MET is overexpressed in PTC and associated with increased locoregional recurrence rates. Perioperative administration of EMI-137 is safe and facilitates NM detection using MFGI and spectroscopy, potentially reducing the number of negative PCLNDs with more than 25%.

Clinical trial registration.
NCT03470259.
Sweet cherry flesh cells burst in non-random clusters along minor veins
Authors: Brinkmann Tobias , Kuhnke Felix , Grimm Eckhard , Knoche Moritz 
Published: April 2022

Abstract
Main conclusion
Sweet cherry flesh cells burst when exposed to water but they do so in clusters indicating heterogeneity with respect to osmotic concentration, which depends on proximity to a minor vein.

Abstract
Water plays a key role in cracking in sweet cherry fruit. Magnetic resonance imaging has previously indicated preferential partitioning of water along veins. A more negative osmotic potential along veins seems the likely explanation. Here we establish if cell bursting in mature sweet cherry fruit is also associated with the veins. Cell bursting was identified by a novel light microscope technique involving exposure of a cut fruit surface to water or to sucrose solutions. Upon exposure to water there was no bursting of skin cells but for cells of the flesh (mesocarp) bursting increased with time. When the cut surface was exposed to sucrose solutions of decreasing osmotic concentrations (increasing water potentials) the incidence of cell bursting increased from hypertonic (no bursting), to isotonic, to hypotonic. Cell bursting in the outer mesocarp occurred primarily in the vicinity of minor veins that in the inner mesocarp was primarily between radial veins. The median distance between a minor vein and a bursting cell (mean diameter 0.129 mm) was about 0.318 mm that between a radial vein and a bursting cell was about 0.497 mm. In contrast, the distance between adjacent minor veins averaged 2.57 mm, that between adjacent radial veins averaged 0.83 mm. Cell bursting tends to occur in clusters. Mapping of cell bursting indicates (1) that a seemingly uniform population of mesocarp cells actually represents a heterogeneous population with regard to their cell osmotic potentials and (2) cell bursting afflicts clusters of neighbouring cells in the vicinities of minor veins.
Molecular mechanisms of skin wound healing in non-diabetic and diabetic mice in excision and pressure experimental wounds
Authors: Baldassarro Vito Antonio , Lorenzini Luca , Giuliani Alessandro , Cescatti Maura , Alastra Giuseppe , Pannella Micaela , Imbimbo Bruno Pietro , Villetti Gino , Calzà Laura , Giardino Luciana 
Published: April 2022

AbstractExperimental models for chronic skin lesions are excision and pressure ulcer, defined as “open” and “closed” lesions, respectively, only the latter characterized by tissue hypoxia. Moreover, systemic diseases, such as diabetes mellitus, affect wound repair. Thus, models for testing new therapies should be carefully selected according to the expected targets. In this study, we present an extensive and comparative histological, immunohistochemical, and molecular characterization of these two lesions in diabetic (db/db) and non-diabetic (C57BL/6 J) mice. In db/db mice, we found significant reduction in PGP9.5-IR innervation, reduction of capillary network, and reduced expression of NGF receptors. We found an increase in VEGF receptor Kdr expression, and the PI3K-Akt signaling pathway at the core of the altered molecular network. Db/db mice with pressure ulcers showed an impairment in the molecular regulation of hypoxia-related genes (Hif1a, Flt1, and Kdr), while extracellular matrix encoding genes (Itgb3, Timp1, Fn1, Col4a1) were upregulated by hyperglycemia and lesions. Overall, the molecular analysis suggests that db/db mice have a longer inflammatory phase of the wound repair process, delaying the progression toward the proliferation and remodeling phases.
Gamma-convergence of a gradient-flow structure to a non-gradient-flow structure
Authors: Peletier Mark A. , Schlottke Mikola C. 
Published: April 2022

AbstractWe study the asymptotic behaviour of a gradient system in a regime in which the driving energy becomes singular. For this system gradient-system convergence concepts are ineffective. We characterize the limiting behaviour in a different way, by proving $$\Gamma $$
Γ
-convergence of the so-called energy-dissipation functional, which combines the gradient-system components of energy and dissipation in a single functional. The $$\Gamma $$
Γ
-limit of these functionals again characterizes a variational evolution, but this limit functional is not the energy-dissipation functional of any gradient system. The system in question describes the diffusion of a particle in a one-dimensional double-well energy landscape, in the limit of small noise. The wells have different depth, and in the small-noise limit the process converges to a Markov process on a two-state system, in which jumps only happen from the higher to the lower well. This transmutation of a gradient system into a variational evolution of non-gradient type is a model for how many one-directional chemical reactions emerge as limit of reversible ones. The $$\Gamma $$
Γ
-convergence proved in this paper both identifies the ‘fate’ of the gradient system for these reactions and the variational structure of the limiting irreversible reactions.
SPINE20 recommendations 2021: spine care for people’s health and prosperity
Authors: Costanzo Giuseppe , Misaggi Bernardo , Ricciardi Luca , AlEissa Sami I. , Tamai Koji , Alhelal Fahad , Alqahtani Yahya , Alsobayel Hana I. , Arand Markus , Balsano Massimo , Blattert Thomas R. , Brayda-Bruno Marco , Busari Jamiu O. , Campello Marco , Chhabra Harvinder S. , Tamburrelli Francesco Ciro , Côté Pierre , Darwono Bambang , Kandziora Frank , La Maida Giovanni A. , Muehlbauer Eric J. , Mulukutla Raghava D. , Pereira Paulo , Rajasekaran Shanmuganathan , Rothenfluh Dominique A. , Sullivan William J. , Truumees Eeric , Dohring Edward J. , Pigott Tim , Shetty Ajoy P. , Teli Marco G. A. , Wang Jeffrey C. , Ames Christopher , Anema Johannes R. , Bang Anand , Cheung Kenneth M. C. , Gross Douglas P. , Haldeman Scott , Minisola Salvatore , Mullerpatan Rajani , Negrini Stefano , Salmi Louis-Rachid , Spinelli M. Silvia , Vlok Adriaan , Yankey Kwadwo P. , Zaina Fabio , Alturkistany Ahmed , Franke Jörg , Liljenqvist Ulf R. , Piccirillo Michael , Nordin Margareta 
Published: April 2022

Abstract
Purpose
The focus of SPINE20 is to develop evidence-based policy recommendations for the G20 countries to work with governments to reduce the burden of spine disease, and disability.

Methods
On September 17–18, 2021, SPINE20 held its annual meeting in Rome, Italy. Prior to the meeting, the SPINE20 created six proposed recommendations. These recommendations were uploaded to the SPINE20 website 10 days before the meeting and opened to the public for comments. The recommendations were discussed at the meeting allowing the participants to object and provide comments.

Results
In total, 27 societies endorsed the following recommendations. SPINE20 calls upon the G20 countries: (1) to expand telehealth for the access to spine care, especially in light of the current situation with COVID-19. (2) To adopt value-based interprofessional spine care as an approach to improve patient outcomes and reduce disability. (3) To facilitate access and invest in the development of a competent rehabilitation workforce to reduce the burden of disability related to spine disorders. (4) To adopt a strategy to promote daily physical activity and exercises among the elderly population to maintain an active and independent life with a healthy spine, particularly after COVID-19 pandemic. (5) To engage in capacity building with emerging countries and underserved communities for the benefit of spine patients. (6) To promote strategies to transfer evidence-based advances into patient benefit through effective implementation processes.

Conclusions
SPINE20’s initiatives will make governments and decision makers aware of efforts to reduce needless suffering from disabling spine pain through education that can be instituted across the globe.
Participatory design and evaluation of virtual reality physical rehabilitation for people living with dementia
Authors: Matsangidou Maria , Frangoudes Fotos , Schiza Eirini , Neokleous Kleanthis C. , Papayianni Ersi , Xenari Katerian , Avraamides Marios , Pattichis Constantinos S. 
Published: April 2022

AbstractEmerging research confirms the need for technologically enhanced solutions to support non-pharmacological interventions which can improve the quality of life, the mental and physical health of demented people. Several types of research examined if virtual reality can be an effective solution. This paper aims to present the cyclic process of prototyping, testing, analysing, and refining the VR system in real-world clinical settings. Seven people with moderate to severe dementia were recruited. The experiment required the patients to attend three virtual reality iterations of rapid prototyping with user testing. All three iterations involved training activities with upper body movements similar to their usual physical training. A mixed-methods design measured affect and emotional behaviour using the Observed Emotion Rating Scale and the Visual Analog Scale. Content analysis was conducted following observations and interviews. During each iteration of rapid prototyping with user testing, quantitative measurements of performance, independence and time were recorded. Eye tracking and movement information were captured by the system. Finally, a simplified version of the presence and usability scales evaluated the system. The results of this study provide further evidence that virtual reality can play a significant role in the improvement of people’s with dementia physical training and emotional health when is appropriately designed. The results present the vital factors which should be incorporated in a virtual reality system which are: 1) a simple interactions modality; 2) visible visual targets and continuous feedback; 3) personalized virtual environments; 4) personalized range of movements.
Numerical Investigation of the Effect of Open Holes on the Impact Response of CFRP Laminates
Authors: Rezasefat M. , Giglio M. , Manes A. 
Published: April 2022

AbstractThe presence of open holes changes the behaviour of composite laminates when subjected to mechanical loads creating critical zones with a high probability of interlaminar and intralaminar damage initiation. While open holes in composite laminates are a requirement in many situations such as assembly needs, wiring, and maintenance access, their influence on the impact response of composite laminates is still poorly understood. In this paper, a numerical study was performed on Carbon Fibre Reinforced Polymer (CFRP) composite laminates with open holes subjected to low velocity impacts. The influence of the distance between open holes to impact origin, hole diameter, and the number of open holes on mechanical response and failure was studied using a FE model based on the inter-fibre failure criterion of Cuntze to account for the progressive intralaminar failure. The interlaminar failure was considered by using zero thickness cohesive elements based on the cohesive zone model. The results showed that i) open holes change the shape and size of the damage caused by low velocity impact and ii) that the presence of an open hole close to the impact origin in-plane spread of damage is stopped resulting in more severe damage and a smaller projected damage area compared to the control specimen. In addition, the presence of open holes in most cases did not change the locality of the low velocity impact but rather changed the severity of the damage in the local impact zone.
Insight into Crosstalk Between Mitophagy and Apoptosis/Necroptosis: Mechanisms and Clinical Applications in Ischemic Stroke
Authors: Yang Yan-di , Li Zi-xin , Hu Xi-min , Wan Hao , Zhang Qi , Xiao Rui , Xiong Kun 
Published: April 2022

AbstractIschemic stroke is a serious cerebrovascular disease with high morbidity and mortality. As a result of ischemia-reperfusion, a cascade of pathophysiological responses is triggered by the imbalance in metabolic supply and demand, resulting in cell loss. These cellular injuries follow various molecular mechanisms solely or in combination with this disorder. Mitochondria play a driving role in the pathophysiological processes of ischemic stroke. Once ischemic stroke occurs, damaged cells would respond to such stress through mitophagy. Mitophagy is known as a conservatively selective autophagy, contributing to the removal of excessive protein aggregates and damaged intracellular components, as well as aging mitochondria. Moderate mitophagy may exert neuroprotection against stroke. Several pathways associated with the mitochondrial network collectively contribute to recovering the homeostasis of the neurovascular unit. However, excessive mitophagy would also promote ischemia-reperfusion injury. Therefore, mitophagy is a double-edged sword, which suggests that maximizing the benefits of mitophagy is one of the direction of future efforts. This review emphasized the role of mitophagy in ischemic stroke, and highlighted the crosstalk between mitophagy and apoptosis/necroptosis.
Fast and Reliable Ag–Sn Transient Liquid Phase Bonding by Combining Rapid Heating with Low-Power Ultrasound
Authors: Rheingans Bastian , Jeurgens Lars P. H. , Janczak-Rusch Jolanta 
Published: April 2022

AbstractAg–Sn transient liquid phase (TLP) bonding is capable of producing joints with excellent mechanical and thermal properties, and with operation temperatures at or even above the processing temperature. However, reduction of the comparatively long processing times required to achieve high-quality joints is highly desirable. To this end, the use of fast heating and low-power ultrasonic (US) pulses for accelerated Ag–Sn TLP bonding is investigated with particular focus on defect generation and avoidance. Employing rapid heating with$$20\,{\text {K s}}^{-1}$$20K s-1, the process time can indeed be strongly reduced, yielding a high average shear strength of$$(58 \pm 22)$$(58±22)MPa. The large variance of the shear strength is caused by gas entrapment and pronounced lateral squeeze-out of liquid Sn, facilitated by the suppression of$${\text{Ag}}_{3} {\text{Sn}}$$Ag3Snformation upon rapid heating. Introduction of a weak US pulse leads to enhanced$${\text{Ag}}_{3} {\text{Sn}}$$Ag3Snformation and efficient removal of entrapped gas, and thus to avoidance of large-scale bond defects, which results in an enhanced shear strength of$$(104 \pm 8)$$(104±8)MPa.
Material dependent surface and subsurface properties of hybrid components
Authors: Budde Laura , Prasanthan Vannila , Merkel Paulina , Kruse Jens , Faqiri Mohamad Yusuf , Lammers Marius , Kriwall Mareile , Hermsdorf Jörg , Stonis Malte , Hassel Thomas , Breidenstein Bernd , Behrens Bernd-Arno , Denkena Berend , Overmeyer Ludger 
Published: April 2022

AbstractTailored forming is used to produce hybrid components in which the materials used are locally adapted to the different types of physical, chemical and tribological requirements. In this paper, a Tailored Forming process chain for the production of a hybrid shaft with a bearing seat is investigated. The process chain consists of the manufacturing steps laser hot-wire cladding, cross-wedge rolling, turning and deep rolling. A cylindrical bar made of mild steel C22.8 is used as the base material, and a cladding of the martensitic valve steel X45CrSi9-3 is applied in the area of the bearing seat to achieve the strength and hardness required. It is investigated how the surface and subsurface properties of the hybrid component, such as hardness, microstructure and residual stress state, change within the process chain. The results are compared with a previous study in which the austenitic stainless steel X2CrNiMo19-12 was investigated as a cladding material. It is shown that the residual stress state after hot forming depends on the thermal expansion coefficients of the cladding material.
Development of a Novel Technique for the Measurement of Neuromuscular Junction Functionality in Isotonic Conditions
Authors: Forconi Flavia , Apa Ludovica , Pisu Simona , Casola Irene , Musarò Antonio , Rizzuto Emanuele , Del Prete Zaccaria 
Published: April 2022

Abstract
Introduction
The neuromuscular junction (NMJ) is a chemical synapse responsible for converting electrical pulses generated by the motor neuron into electrical activity in muscle fibers, and is severely impaired in various diseases, such as Amyotrophic Lateral Sclerosis (ALS). Here, we proposed a novel technique to measure, for the first time, NMJ functionality in isotonic conditions, which better reflect muscle physiological activity.

Methods
We employed the in-situ testing technique, studied a proper placing of two pairs of wire electrodes for nerve and muscle stimulation, developed an extensive testing protocol, and proposed a novel parameter, the Isotonic Neurotransmission Failure (INF), to properly capture the impairments in neurotransmission during isotonic fatigue. We employed wild-type mice to assess the feasibility of the proposed technique, and the ALS model SOD1G93A mice to demonstrate the validity of the INF.

Results
Results confirmed the measurement accuracy in term of average value and coefficient of variation of the parameters measured through nerve stimulation in comparison with the corresponding values obtained for membrane stimulation. The INF values computed for the SOD1G93A tibialis anterior muscles pointed out an impairment of ALS mice during the isotonic fatigue test, whereas, as expected, their resistance to fatigue was higher.

Conclusions
In this work we devised a novel technique and a new parameter for a deep assessment of NMJ functionality in isotonic conditions, including fatigue, which is the most crucial condition for the neuronal signal transmission. This technique may be applied to other animal models, to unravel the mechanisms behind muscle-nerve impairments in other neurodegenerative pathologies.