bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new sensitive and selective detection of Ga3+ by thiophene‐based ‘turn‐on’ fluorescent chemosensor
Authors: Heo Jae Sung , Gil Dongkyun , Kim Cheal 
Published: February 2022

AbstractWe designed a thiophene‐based fluorescent chemosensor DHTC ((E)‐2‐([3,5‐dichloro‐2‐hydroxybenzylidene]amino)thiophene‐3‐carboxamide) for detecting gallium (Ga3+). DHTC could probe Ga3+ using fluorescence enhancement. The limit of detection for Ga3+ by DHTC was 0.39 μM. The binding mode of DHTC to Ga3+ was determined as a 1:1 ratio from analysis by Job’s plot and electrospray ionization‐mass spectrometry (ESI‐MS). In addition, DHTC could selectively detect Ga3+ using test kits. The sensing process of Ga3+ by DHTC was presented using ultraviolet–visible light titration, Job’s plot, ESI‐MS, 1H nuclear magnetic resonance titration, and density functional theory calculation.
Utilization of N,S‐doped carbon dots as a fluorescent nanosensor for determination of cromolyn based on inner filter effect: application to aqueous humour
Authors: Abd Elhaleem Shymaa M. , Elsebaei Fawzi , Shalan Shereen , Belal Fathalla 
Published: February 2022

AbstractA simple and eco‐friendly hydrothermal technique is used to prepare water soluble N‐ and S‐co‐doped carbon quantum dots probes (N,S‐CQDs) from thiosemicarbazide and citric acid. Several characterization techniques were performed to ensure the successful synthesis of highly luminescent N,S‐CQDs. The prepared probe exhibited analytical potential as an optical nanosensor for the spectrofluorimetric determination of cromolyn sodium (CRO) in its pharmaceutical dosage forms and aqueous humour. The emission intensity of the synthesized N,S‐CQDs was measured at 411 nm after excitation at 345 nm. Addition of increasing concentrations of CRO to N,S‐CQDs led to quenching of its fluorescence intensity. CRO was investigated within a wide concentration range 10.0–150.0 μM with a limit of detection of 2.0 μM and a limit of quantification of 6.0 μM. The quenching of fluorescent N,S‐CQDs occurred through the inner filter effect (IFE). The developed spectrofluorimetric method was successfully optimized and validated according to the International Council of Harmonisation guidelines (ICH). The method greenness is proved through using both Eco‐Scale and AGREE approaches.
The role of the CRISPR‐Cas system in cancer drug development: Mechanisms of action and therapy
Authors: Chandrasekaran Arun Pandian , Karapurkar Janardhan Keshav , Chung Hee Yong , Ramakrishna Suresh 
Published: February 2022

AbstractBackgroundThe recent emergence of gene editing using Clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR associated system (Cas) tools and advances in genomics and proteomics has revolutionized drug discovery and personalized medicine.Purpose and ScopeThe CRISPR‐Cas system has enabled gene and cell‐based therapies, screening for novel drug targets, a new generation of disease models, elucidation of drug resistance mechanisms, and drug efficacy testing. Here, we summarized recent investigations and strategies involved in cancer‐related drug discovery using the CRISPR‐Cas system.ConclusionCRISPR‐Cas‐mediated gene editing has shown great potential in the development of next generation drugs for treatment of Mendelian disorders and various cancer types. In this review, we focused on the impact of the CRISPR‐Cas system in drug discovery and its application to biomarker identification and validation, high‐end target genes, and breakthrough anticancer cell therapies. We also highlighted the role of CRISPR‐Cas in precision disease modeling and functional drug screening.
Metabolomic comparison between natural Huaier and artificial cultured Huaier
Authors: Jian Tongtong , Zhang Yu , Zhang Guoying , Ling Jianya 
Published: February 2022

AbstractVanderbylia robiniophila (Murrill) B.K. (Huaier) is a kind of higher fungal fruiting body that is parasitic on the trunk of Sophora japonica and Robinia pseudoacacia L.. As a traditional Chinese medicine with a history of more than 1,600 years, Huaier has attracted wide attention for its excellent anticancer activity. A systematic study on the metabolome differences between natural Huaier and artificial cultured Huaier was conducted using liquid chromatography–mass spectrometry in this study. Principal component analysis and orthogonal projection on latent structure‐discriminant analysis results showed that cultured Huaier evidently separated and individually separated from natural Huaier, indicating metabolome differences between natural and cultured Huaier. Hierarchical clustering analysis was further performed to cluster the differential metabolites and samples based on their metabolic similarity. The higher contents of amino acids, alkaloids and terpenoids in natural Huaier make it an excellent choice as a traditional Chinese medicine for anticancer or nutritional supplementation. The results of the Bel‐7,402 and A549 cell cytotoxicity tests showed that the anticancer activity of natural Huaier was better than that of cultured Huaier. This may be due to the difference in chemical composition, which makes the anticancer activities of natural and cultured Huaier different.
Transgender identity and experiences of sexual harassment in adolescence
Authors: Kaltiala Riittakerttu , Ellonen Noora 
Published: February 2022

AbstractGender‐diverse adolescents have reported excessive experiences of sexual harassment, but studies on this are few and suffer from methodological problems. Our aim was to compare experiences of sexual harassment between adolescents belonging to different gender‐identity groups. A nationally representative cross‐sectional survey of 127,210 adolescents aged 14–20 in Finland in 2017 was used. The respondents were pupils in the eighth and ninth grades of the compulsory nine‐year comprehensive school with mean (sd) age of 14.83 (0.82) years and students in upper secondary school (age: mean (sd) 16.84 (0.83) years) and vocational school (17.29 (2.43) years). Cisgender, opposite sex‐identifying and non‐binary adolescents were compared. Self‐reports of experiences of sexual harassment, emotional symptoms and externalising behaviours (bullying perpetration, frequent alcohol use, truancy, drug use) were elicited. The data were analysed using cross‐tabulations and logistic regression. When confounders were controlled for, odds ratios were increased for gender harassment (OR 2.0), unwelcome sexual attention (OR 1.7) and sexual coercion (OR 2.0) among adolescents with non‐binary gender identity, and for gender harassment (OR 2.1) among those identifying with the opposite sex. Subjection to sexual harassment is particularly associated with non‐binary gender identity. This may reflect that others use sexual harassment as a form of control of gender‐nonconforming self‐expression.
Design, Synthesis, in Vitro, and in Silico Evaluation of N‐Phenylacetamide‐Oxindole‐Thiosemicarbazide Hybrids as New Potential Tyrosinase Inhibitors
Authors: Yari Boroujeni Shahriar , Haghighijoo Zahra , Mohammadi‐Khanaposhtani Maryam , Mosadeghkhah Ali , Moazzam Ali , Yavari Ali , Hajimahmoodi Manan , Sabourian Reyhaneh , Hosseini Samesadat , Larijani Bagher , Hamedifar Halleh , Ansari Samira , Mahdavi Mohammad 
Published: February 2022

AbstractA novel series of N‐phenylacetamide‐oxindole‐thiosemicarbazide hybrids were synthesized and evaluated for their tyrosinase inhibitory activity. According to tyrosinase inhibition results, all the synthesized compounds showed high tyrosinase inhibitory activity with IC50 values ranging from 0.8 to 3.88 μM in comparison to positive control kojic acid with IC50 value of 36.32 μM. Among tested compounds, analog 7o, containing the 2‐methyl‐4‐nitrophenyl on N‐phenylacetamide moiety displayed superior tyrosinase inhibition. This compound was around 45‐fold more potent than kojic acid. The kinetic analysis of compound 7o demonstrated that this compound is a competitive inhibitor against tyrosinase. Docking study of this compound demonstrated that compound 7o interacted with critical histidine residues within tyrosinase active site.
Usefulness of robot‐assisted total knee arthroplasty in patients with retained hardware: A report of two cases
Authors: Baek Ji‐Hoon , Lee Su Chan , Ahn Hye Sun , Nam Chang Hyun 
Published: February 2022

AbstractTotal knee arthroplasty (TKA) is technically demanding in patients with pre‐existing hardware around the knee. We report two cases of knee osteoarthritis in patients with retained hardware who would have difficulty undergoing conventional TKA. Robot‐assisted TKA can be a useful treatment for knee osteoarthritis in patients with retained hardware.
Peculiar thousand leaves soft‐tissue mass: Elastofibroma dorsi
Authors: Limaiem Faten , Baccouch Seifeddine , Hajri Mohamed 
Published: February 2022

AbstractElastofibroma dorsi is an uncommon benign soft‐tissue tumor with uncertain pathogenesis (Medicina (Kaunas), 2021; 57, 370). Surgical excision is the therapeutic option for symptomatic patients. A pathological study confirms the diagnosis after surgery. The prognosis is excellent with extremely rare recurrence cases (Anticancer Res, 2021, 41, 2211).
Microkinetic Modeling of Acetylene Hydrogenation Under Periodic Reactor Operation
Authors: Urmès Caroline , Daniel Cécile , Schweitzer Jean‐Marc , Cabiac Amandine , Julcour Carine , Schuurman Yves 
Published: February 2022

AbstractDynamic reactor operations will gain interest with the increase of intermittent electricity from renewable sources. Periodic operation of catalytic reactors can also be used for kinetic studies with better parameter estimation than steady‐state operation. This methodology is exemplified for the case of acetylene hydrogenation over Pd/α‐Al2O3 using small amplitude flow oscillations. It allows the evaluation of several elementary kinetic parameters from one set of dynamic data for a reaction mechanism based on a single catalytic site. The optimized transient model is able to reproduce the time‐evolution of the reactant and product molar fractions at the outlet of a fixed bed operated with modulations of different amplitudes and frequencies. It further shows that the acetylene conversion is controlled by two steps, the hydrogen adsorption step and the hydrogenation of the surface vinyl intermediate into adsorbed ethylene.
Drug‐Drug Interaction of Letermovir and Atorvastatin in Healthy Participants
Authors: McCrea Jacqueline B. , Menzel Karsten , Adedoyin Adedayo , Cho Carolyn R. , Fox‐Bosetti Sabrina , Macha Sreeraj , Zhao Tian , Liu Fang , Panebianco Deborah , Stoch S. Aubrey , Iwamoto Marian 
Published: February 2022

AbstractLetermovir (MK‐8228/AIC246) is a cytomegalovirus (CMV) DNA terminase complex inhibitor for CMV prophylaxis in adult patients undergoing hematopoietic stem cell transplant. It is cytochrome P450 (CYP) 3A inhibitor and inhibits organic anion transporting polypeptide 1B1/3 and breast cancer resistance protein transporters. Atorvastatin (ATV), a commonly used treatment for hypercholesterolemia, is a substrate of organic anion transporting polypeptide 1B1, potentially breast cancer resistance protein, and CYP3A. As letermovir may be coadministered with ATV, the effect of multiple‐dose letermovir 480 mg once daily on the pharmacokinetics of single‐dose ATV 20 mg and its metabolites (ortho‐hydroxyatorvastatin [o‐OH‐ATV] and para‐hydroxyatorvastatin [p‐OH‐ATV]) was evaluated in an open‐label trial in healthy female adults (N = 14). ATV area under the plasma concentration–time curve from time 0 to infinity and maximum plasma concentration (Cmax) increased ≈3‐fold with letermovir coadministration. The time to ATV Cmax also increased, while apparent clearance decreased. The exposures of o‐OH‐ATV and p‐OH‐ATV were comparable in the presence versus absence of letermovir; however, o‐OH‐ATV Cmax decreased by 60% with coadministration, while p‐OH‐ATV Cmax was similar. Due to the increase in ATV exposure with letermovir coadministration, statin‐associated adverse events such as myopathy should be closely monitored following coadministration. The dose of ATV should not exceed 20 mg daily when coadministered with letermovir.
Four‐Way Crossover Phase 1 Thorough QT Study to Assess Cardiac Safety of Domperidone and Consideration for Design and Conduct of QT/QTc Study in China
Authors: Wang Fangfang , Nie Jing , Yang Li , Yang Zhenhua , Xu Lin , Wang Liqun , Liao Huimin , Li Mengyu , Wang Ping , Li Gailing , Li Haiyan 
Published: February 2022

AbstractThis study aimed to evaluate the QT prolongation potential of domperidone in healthy Chinese participants and explore the possibility of a thorough QT (TQT) study in China with a smaller sample size using concentration‐QT (C‐QT) modeling. Part 1 was a randomized, placebo‐ and positive‐controlled, multiple‐dose, 4‐way crossover TQT study in healthy Chinese participants; 44 participants were randomized to either domperidone 10/20 mg or placebo 3 times daily, on days 1 to 3, followed by a single dose of either 10/20 mg domperidone/domperidone‐placebo/domperidone‐placebo plus 400 mg of moxifloxacin, on day 4. Twelve‐lead electrocardiograms were recorded in triplicate at predefined time points with pharmacokinetic sampling. The results were that change from baseline in QT interval corrected for heart rate (QTc) using the Fridericia formula (QTcF) between domperidone and placebo was 1.3 milliseconds and 2.7 milliseconds for 10 and 20 mg 3 times daily, and upper limits of 2‐sided 90%CI for all time points were below regulatory threshold of 10 milliseconds. In part 2, resampling analysis using C‐QT modeling for moxifloxacin showed false‐negative rates of <5% with sample sizes ≥6. We could conclude that no clinically relevant effect on corrected QT interval or new safety signals was observed with domperidone. A dedicated TQT study with C‐QT modeling could assess drug effects on QT/corrected QT intervals for novel drug development in China.
Smart Drug Delivery Systems Based on DNA Nanotechnology
Authors: Fan Qin , He Zhimei , Xiong Jinxin , Chao Jie 
Published: February 2022

AbstractThe development of DNA nanotechnology has attracted tremendous attention in biotechnological and biomedical fields involving biosensing, bioimaging and disease therapy. In particular, precise control over size and shape, easy modification, excellent programmability and inherent homology make the sophisticated DNA nanostructures vital for constructing intelligent drug carriers. Recent advances in the design of multifunctional DNA‐based drug delivery systems (DDSs) have demonstrated the effectiveness and advantages of DNA nanostructures, showing the unique benefits and great potential in enhancing the delivery of pharmaceutical compounds and reducing systemic toxicity. This Review aims to overview the latest researches on DNA nanotechnology‐enabled nanomedicine and give a perspective on their future opportunities.
Comparing self‐compassion versus body exposure for adult women with moderate to severe body dissatisfaction: A feasibility and pilot trial
Authors: Turk Fidan , Kellett Stephen , Waller Glenn 
Published: February 2022

AbstractThis study assessed the feasibility and effectiveness of two brief online interventions for body shame for women with moderate to severe negative body image, to inform the design of a future randomized controlled trial. The primary feasibility outcomes were recruitment, measure completion rates, retention rates and internet connection failure rates. The secondary pilot outcomes were change on clinical measures and state shame ratings during the interventions. Participants were randomized to either online (40‐min single session) body exposure or self‐compassion interventions. Five validated nomothetic outcome measures (body dissatisfaction, appreciation, eating disorder, external shame and anxiety) were taken at three time points (preintervention, postintervention and 2‐week follow‐up). Subjective units of body shame (SUBS 0–100 scale) were rated every 5 min during the interventions. The target of recruiting 30 participants in 60 days was successfully achieved. The measure completion rate was high (100%), and retention rates (80% to 100%) showed moderate‐to‐high acceptability of the interventions. Online delivery was moderately viable with a 12.5% session disconnection rate. The self‐compassion intervention significantly reduced SUBS ratings during the course of the intervention, but there was no significant improvement or difference between the interventions on nomothetic outcome measures. Findings suggest that a fully powered trial is viable, and sample size calculation and methodological requirements are provided.
Oligo‐FISH Paints in Triticeae
Authors: Li Guangrong , Yang Zujun 
Published: February 2022

AbstractWe developed seven oligonucleotide (oligo) pools based on single‐copy sequences, targeting chromosomes 1 to 7 of barley (Hordeum vulgare L.) and wheat (Triticum aestivum) for chromosomal Oligo‐FISH painting methods. The probes were applied to high‐throughput karyotyping for the Triticeae tribe of over 350 species including 30 genera such as Triticum, Hordeum, Secale, Aegilops, Thinopyrum, and Dasypyrum, as well as several wheat alien–derived lines. In combination with other nondenaturing FISH (ND‐FISH) procedures using tandem‐repeat oligos, the newly developed Oligo‐FISH painting technique provides an efficient tool for the identification of individual chromosomes with homologous linkage groups to establish standard karyotypes, particularly with any wild Triticeae species having nonsequenced genomes for chromosome evolutionary analysis. © 2022 Wiley Periodicals LLC.Basic Protocol 1: Oligo‐pool probe developmentBasic Protocol 2: Nondenaturing FISHBasic Protocol 3: Oligo‐FISH painting
CRISPR‐Act3.0‐Based Highly Efficient Multiplexed Gene Activation in Plants
Authors: Pan Changtian , Qi Yiping 
Published: February 2022

AbstractCRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein)‐mediated genome editing has revolutionized fundamental research and plant breeding. Beyond gene editing, CRISPR/Cas systems have been repurposed as a platform for programmable transcriptional regulation. Catalytically inactive Cas variants (dCas), when fused with transcriptional activation domains, allow for specific activation of any target gene in the genome without inducing DNA double‐strand breaks. CRISPR activation enables simultaneous activation of multiple genes, holding great promise in the identification of gene regulatory networks and rewiring of metabolic pathways. Here, we describe a simple protocol for constructing a dCas9‐mediated multiplexed gene activation system based on the CRISPR‐Act3.0 system. The resulting vectors are tested in rice protoplasts. © 2022 Wiley Periodicals LLC.Basic Protocol 1: sgRNA design and construction of CRISPR‐Act3.0 vectors for multiplexed gene activationBasic Protocol 2: Determining the activation efficiency of CRISPR‐Act3.0 vectors using rice protoplasts
Through‐Space Electrostatic Effects of Positively Charged Substituents on the Hydrogen Evolution Reaction
Authors: Zhang Qingxin , Lei Haitao , Guo Hongbo , Wang Yabo , Gao Yimei , Zhang Wei , Cao Rui 
Published: February 2022

AbstractElucidating the effects of various structural components on energy‐related small molecule activation is of fundamental and practical significance. Herein the inhibition effect of positively charged substituents on the hydrogen evolution reaction (HER) was reported. With the use of Cu porphyrins1–5containing different numbers and locations of positively charged substituents, it was demonstrated that their electrocatalytic HER activities significantly decreased when more cationic units were located close to the Cu ion: theicat/ip(icatis the catalytic peak current,ipis the one‐electron reduction peak current) value decreased from 38 with zero cationic unit to 15 with four closely located cationic units. Inspired by this result, Cu porphyrin6, with fourmeso‐phenyl groups each bearing a negatively chargedpara‐sulfonic substituent, was designed. With these anionic units,6outperformed the other Cu porphyrins for electrocatalytic HER under the same conditions.
Modal analysis of an arch dam combining ambient vibration measurements, advanced fluid‐element method and modified engineering approach
Authors: Guo Xiangfeng , Dufour Frédéric , Humbert Nicolas 
Published: February 2022

AbstractModal analysis, aiming at estimating modal characteristics such as natural frequencies and mode shapes, is fundamental for studying the dynamic behaviours of a structure. This paper presents a modal analysis of an arch dam using three different techniques. The reference one is based on the statistical analysis of the ambient vibration data collected from the dam crest. The two other approaches are both numerical but with different methods (fluid‐element and Westergaard) for the modelling of the reservoir and its interactions with the dam. By applying the three techniques to the studied dam and comparing their results, it is demonstrated that: (1) analysing the ambient vibration data through an operational modal analysis method is able to extract the dam modal characteristics; (2) the fluid‐element method is effective for arch dams since the first 10 natural frequencies can be accurately predicted once the material parameters are calibrated on the first three modes; and (3) the Westergaard method, a technique with only additional masses, produces significantly under‐estimated frequencies for the first few modes if same parameters are used as the fluid‐element method; the underestimation can be corrected for several modes by using a higher stiffness parameter but the required value is unrealistic for the case study. Furthermore, a modified Westergaard method is introduced in this paper by using a reduced added‐mass coefficient. This method, once the coefficient is calibrated on the 1st mode, is able to well predict the partially coupled modes as illustrated with the case study of the Saint‐Guérin dam.
Rekiske: Interdisciplinary platform for sharing knowledge and experience of Japanese historical documents
Authors: Ichino Mika , Masuda Kooiti 
Published: February 2022

AbstractHistorical materials contain descriptions of various events involved in natural processes and human societies (such as weather, earthquakes, volcanic eruptions, harvests, market prices, and famines). They have been used for studies in diverse fields, including natural sciences (such as seismology, climatology, and astronomy), social sciences, and humanities. Hence, researchers have made various efforts, such as identifying and obtaining the materials, reading handwritten characters and transcribing them into digital texts, standardizing formats, extracting descriptions of relevant phenomena, and constructing integrated datasets, to use documentary information. Because scientists, other than historians, find it quite challenging even to ascertain where to find appropriate research materials, the Rekiske Development Group has, therefore, developed a system called Rekiske to facilitate interdisciplinary sharing of information available from historical materials. Rekiske is designed for sharing bibliographic and scientific information of historical materials, along with researchers’ experience and knowledge, through interdisciplinary collaboration. Rekiske can assist researchers in identifying and obtaining useful historical materials for research purposes. It also facilitates collaboration among experts to share their expertise and knowledge of historical information, materials, and recent studies. Using Rekiske, we register various types of information on historical materials regarding weather or earthquakes considered in geoscience research to utilize researchers’ experiences and to produce new knowledge through interdisciplinary collaborations. The developed system is designed to be used as a metadata portal and pathfinder to promote and preserve historical data and facilitate an understanding of the spatiotemporal connection between nature and human society. We have applied Rekiske to historical climatic studies and discuss its usage, benefits, usability, and other remaining issues.