bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stratifin indicates worse prognosis and promotes hepatocellular carcinoma proliferation, migration, invasion and EMT by modulating Wnt/β-catenin pathway
Authors: Ye Shanping , Li Taiyuan , Shi Jun , Cheng Xiaoye 
Published: January 2022

Abstract
Purpose: Stratifin (SFN) is closely related to the development of cancers, but the biological role and mechanism of SFN in hepatocellular carcinoma (HCC) remain largely unknown and its function in Wnt/β-catenin/EMT pathway in HCC is currently unclear. Methods: Protein and mRNA expression levels of SFN were detected in HCC and corresponding adjacent non-tumor tissues by western blot and RT-qPCR. The correlation of clinicopathological features and SFN was analyzed. The univariate and multivariate analysis were performed to investigate the prognosis value of SFN in HCC patients based on online database. Artificial modulation of SFN was conducted to explore the role of SFN on HCC cells’ proliferation, migration, invasion in vitro, tumor growth in vivo, epithelial mesenchymal transformation (EMT) and activation of Wnt/β-catenin signaling. Results: SFN was up-regulated in HCC cell lines and tissues. Clinically, SFN was positively associated with tumor size, degree of differentiation, TNM stage and vascular invasion. Patients with high SFN levels had worse OS and DFS. Biologically, knockdown of SFN repressed tumor cell proliferation, migration, invasion in vitro and tumor growth in vivo. Conversely, over-expression of SFN promoted these effects. Mechanistically, SFN induced EMT and activated Wnt/β-catenin pathway, while depletion of SFN inhibited EMT and activation of Wnt/β-catenin pathway. Conclusions: SFN indicates worse survival in HCC patients and promotes HCC cells’ proliferation, migration and invasion. Mechanistically, SFN induces EMT by activating Wnt/β-catenin pathway. Our results clarify the role and potential mechanism of SFN in HCC, which may provide a prognostic factor and therapeutic target for HCC patients.
Transcription Analysis of Chicken Embryo Fibroblast Cells Infected with a Recombinant Avian Leukosis Virus Isolate GX14FF03
Authors: Wang Peikun , Li Qiuhong , Wang Jing , Deng Qiaomu , Li Min , Wei Ping 
Published: January 2022

Abstract
Infection with recombinant avian leukosis virus (ALV) has previously been linked to malignancies and immunosuppression. However, the processes behind recombinant ALV's unique pathophysiology are poorly understood. The study aims to investigate the gene expression patterns of a recombinant ALV isolate GX14FF03 infected chicken fibroblast cells (CEFs) and used the RNA-Sequence technique to undertake a complete analysis of the mRNAs transcriped. As a consequence, a total of 907 significant differentially expressed genes (SDEGs) were identified. Among these SDEGs, the most significantly up-regulated gene was interleukin 8-like 1 (IL8L1), while the most significantly down-regulated gene was fibroblast growth factor 16 (FGF16). The 907 SDGEs were highly enriched (p < 0.05) for 252 Gene Ontology (GO) terms, including 197 BP, 3 CC, and 52 MF. According to the KEGG data analysis, SDEGs are implicated in eight significant pathways (p < 0.05). Furthermore, PPI network analyses revealed that IL8L1 interacts with 17 genes. These findings provide light on the molecular mechanisms of the recombinant ALV infection by explaining the mRNA expression profile in CEFs infected with GX14FF03 virus.
Outcomes in terms of morbidity and mortality among elderly head injury patients on pre-injury anticoagulation: A systematic review protocol
Authors: Ogbu Ikenna Idika 
Published: January 2022

Abstract
Background: The global burden of traumatic brain injuries continues to remain grossly underestimated and is thought to cost about US$400 billion annually. Elderly individuals have the highest age-specific rates of TBIs and are more likely to take anticoagulant medication than other age groups and are therefore theoretically at a higher risk of morbidity and mortality following head injuries. However, the evidence for these worse outcomes is at best unclear, and this has a significant impact on decision-making in clinical settings, sometimes resulting in unnecessary delays to treatment.Methods: This protocol has been developed using the PRISMA-P guidelines. We will search the main clinical indexing databases (including PubMed/MEDLINE, EMBASE, Cochrane reviews, and ClinicalTrials.gov) for studies that evaluated outcomes in terms of morbidity and mortality among anticoagulated individuals aged over 65, to identify all English language studies published between the years 2000 and 2021. The identified studies will then be screened using pre-determined eligibility criteria detailed in the body of this work. Finally, two independent reviewers will screen, select, evaluate the included studies for quality, and extract data related to the use of anticoagulants in the elderly from all relevant sources. A logically sound analytic framework will then be employed to determine the impact of the use of anticoagulation on outcomes as outlined. Discussion: This study aims to identify the existing evidence for outcomes in this population, contribute to the current body of knowledge, and provide more explicit guidance for clinical practice. We envisage that the findings surrounding outcomes in the head-injured, anticoagulated elderly population will guide clinicians towards better decision-making in both the in-patient and out-patient setting and contribute to the development of clinical guidelines. Systematic review registration: This systematic review is registered on the PROSPERO database with registration number: CRD42021220974
Identifying and addressing conflicting results across multiple discordant systematic reviews on the same question: Protocol for a replication study of the Jadad algorithm
Authors: Lunny Carole , Thirugnanasampanthar Sai Surabi , Kanji Salman , Ferri Nicola , Pieper Dawid , Whitelaw Sera , Thabet Pierre , Tasmin Sara , Nelson Harrison , Reid Emma , Zhang Jia He (Janet) , Kalkat Banveer , Chi Yuan , Thompson Jacqueline Y , Abdoulrezzak Reema , Zheng Di Wen (Wendy) , Pangka Lindy R.S. , Wang Dian (Xin Ran) , Safavi Parisa , Sooch Anmol , Kang Kevin T. , Tricco Andrea C 
Published: January 2022

Abstract
Introduction: An increasing growth of systematic reviews (SRs) presents notable challenges for decision-makers seeking to answer clinical questions. In 1997, an algorithm was created by Jadad to assess discordance in results across SRs on the same question. Our study aims to (a) replicate assessments done in a sample of studies using the Jadad algorithm to determine if the same SR would have been chosen, (b) evaluate the Jadad algorithm in terms of utility, efficiency, and comprehensiveness, and (c) describe how authors address discordance in results across multiple SRs. Methods and Analysis: We will use a database of 1218 overviews (2000-2020) created from a bibliometric study as the basis of our search for studies assessing discordance (called Discordant Reviews). This bibliometric study searched MEDLINE (Ovid), Epistemonikos, and Cochrane Database of Systematic Reviews for overviews. We will include any study using Jadad (1997) or another method to assess discordance. The first 30 studies screened at the full-text stage by two independent reviewers will be included. We will replicate the authors’ Jadad assessments. We will compare our outcomes qualitatively and evaluate the differences between our Jadad assessment of discordance and the authors’ assessment. Ethics and Dissemination: No ethics approval was required as no human subjects were involved. In addition to publishing in an open-access journal, we will disseminate evidence summaries through formal and informal conferences, academic websites, and across social media platforms. This is the first study to comprehensively evaluate and replicate Jadad algorithm assessments of discordance across multiple SRs.
Development of a Nanoformulation for Oral Protein Administration: Characterization and Preclinical Orofacial Antinociceptive Effect
Authors: Damasceno Marina de Barros Mamede Vidal , Santos Sacha Aubrey Alves Rodrigues , Araújo João Ronielly Campêlo , Barroso Lana Karine Vasconcelos , Benevides Samara Casemiro , Magalhães Francisco Ernani Alves , Tavares Kaio César Simiano , Moreira Renato de Azevedo , Monteiro-Moreira Ana Cristina de Oliveira , Silva Angelo Roncalli Alves e , Campos Adriana Rolim 
Published: January 2022

Abstract
Nanoencapsulation is a valid alternative for the oral administration of peptide drugs and proteins, as nanoparticles protect them from proteolytic degradation in the gastrointestinal tract and promote the absorption of these macromolecules. The orofacial antinociceptive effect of frutalin (FTL), through the intraperitoneal route, has already been proven. This study aimed to develop, characterize, and evaluate the orofacial antinociceptive activity of an oral formulation containing FTL in acute and neuropathic preclinical tests. The analyzed sample did not show any cytotoxicity; 52.2% of the FTL was encapsulated, the size of the nanocapsules was less than 200 nm, the polydispersion was 0.361 and the zeta potential was - 5.87 millivolts. Nanoencapsulated FTL administered by oral route had an orofacial antinociceptive effect in acute and neuropathic rodent models. The antinociceptive effect of FTL was prevented by ruthenium red, but not by camphor. FTL reduced Trpv1 gene expression. FTL promotes orofacial antinociception probably due to the antagonism of TRPV1 channels and the nanoformulation represents an effective method of administering this protein orally.
Strength and vibration analysis of transonic compressor under multiple field loads
Authors: Feng Yu Ru , Wang Jun Li , Li Jin Yang 
Published: January 2022

The transonic axial flow compressor Rotor 37 is subjected to the combined action of thermal load, centrifugal load, and aerodynamic load in real work. The difference in load will cause the blade deformation and the size and distribution of stress to change. And it will cause the vibration frequency of the blade to change compared with the static state at room temperature. This paper studies the compressor blades based on the fluid-thermal-structure coupling method, and comprehensively considers the combined effects of thermal load, centrifugal load and aerodynamic load on the compressor blades. The results of the study show: that when the thermal load acts on the blade alone, it will have less impact on the blade deformation and greater impact on the stress, but when coupled with other loads, it will have a greater impact on the blade deformation and stress. In the case of fluid-thermal-structure coupling, the deformation of the compressor blade will first increase and then decrease with the increase of mass flow, and the change of stress will not decrease with the increase of mass flow. Rotation speed will affect the change trend of blade stress with mass flow. Compared with the static state at room temperature, the natural frequency of the blade under fluid-thermal-structure coupling has undergone a huge change. The first-order vibration frequency has increased by 25.77 %. It can be seen from the Campbell diagram that the compressor blade has a resonance at about 63 % and 98 % Point, in this case, the blade is prone to resonance, and more attention should be paid.
Evaluation and projection of a snow coverage rate over the Upper Yarkant River Basin, China
Authors: Wei Renjuan , Peng Liang , Wu Yuxin , Liang Chuan , Zhao Lu 
Published: January 2022

Abstract
Based on snow coverage remote sensing data NOAA, the simulation ability of the snow coverage rate from the core experiments of the 5th Coupled Model Intercomparison Project (CMIP5) over the Upper Yarkant River Basin was evaluated. The ensemble mean of the eight models was used to project future changes in the snow coverage rate over the basin under the three different representative concentration pathways (RCP2.6, RCP4.5 and RCP8.5). The results show that the snow coverage rate of the eight models can basically simulate a sharp drop of the spatial distribution from the west to the northeast; all the eight models have the capacity to simulate the snow coverage rate of the Yarkant River Basin analyzed from the correlation analysis (R), the ratio of spatial standard deviation (SDR) and the S index. The simulation capacity of the eight models in spring, autumn and winter is stronger than the one in summer, and the ensemble mean of the eight models simulates the best. Under the scenarios of RCP2.6 and RCP4.5, changes in the snow coverage rate show negative differences in most areas over the basin, only the local part shows positive differences. Under the scenario of RCP8.5, changes in the snow coverage rate in all seasons present negative differences in the whole basin, and the decreasing trends are more obvious. Under the same emission scenario, the negative differences in winter are weaker than those of other seasons, and the most significant negative difference appears in spring and autumn. The projection scenarios indicate a clearly decreasing trend under the different emission scenarios. Under the scenario of RCP2.6, the decreasing trend is not obvious as it fails the 90% confidence level test. Under the scenarios of RCP4.5 and RCP8.5, the decreasing trend of the snow coverage rate is significant, passing the 99% confidence level test.
Investigating streamflow variability and hydroclimatic teleconnections: a comparison of regulated and unregulated streamflow stations
Authors: Pant Tarun , Ganapathy Abinesh , Agarwal Ankit 
Published: January 2022

Abstract
This study evaluates the relationship between flow variability of unregulated and regulated streamflow stations and global climate indicators. Mann–Kendall and change-point analysis is applied to investigate the gradual and abrupt changes in streamflow data, followed by the investigation of multi-scale fluctuations in streamflow data using Continuous Wavelet Analysis. Linkages between streamflow and global climate indicators are examined using Cross-Wavelet and Wavelet Coherence Analysis. Results showed contrasting trend values for unregulated and regulated streamflow stations. Surprisingly, all unregulated stations experienced a significant abrupt shift in change point contrary to the regulated streamflow. Further, for unregulated stations, streamflow variability and hydroclimatic teleconnections were observed at a lower scale, indicating that variations in streamflow are more frequent and generally occur on an intra-annual to inter-annual scale. Contrary to this, regulated stations observed the streamflow variability and hydroclimatic teleconnections at a larger scale (8–10 years), indicating that all the fluctuations are smoothed out. Thus, unregulated stations cannot be used as a proxy for regulated stations in any given basin. Indeed, for better water resource planning and management, both regulated and unregulated streamflow should be investigated.
Experimental study on the water–rock interaction mechanism in a groundwater heat pump reinjection process
Authors: Yang Jie , Tao Yuezan , Gao Yulan , Wang Lijuan , Kang Bo 
Published: January 2022

Abstract
Groundwater heat pump (GWHP) is a clean new energy technology. However, recharge clogging has always affected the operational efficiency of GWHP systems. The mechanism of the water–rock interaction inducing the chemical blockage of aquifers in particular is not clear enough. In this study, a sand column device was designed to simulate the recharge of GWHP, and the geothermal water and aquifer sand of the actual GWHP project were collected. Moreover, we have characterized the sand using SEM-EDS, XRD and FT-IR; meanwhile, the evolution of the hydrochemical components, the relationship between TDS and mineral dissolution and the concentration variation trend of [Na+ + K+] and [Mg2+ + Ca2+] were analysed. The results showed that the maximum reduction of the albite content in the column, except for P4 and P6, was 13.97%, while the calcite content in the P3–P4 and P7–P10 segments increased by 1.2%. The anhydrite content was reduced in the whole interval. Therefore, the precipitation and dissolution of minerals might occur in the process of recharge, which was more significant in the front of the column. In addition, the water–rock reaction induced by GWHP recharge is a process that also involves the cation exchange adsorption of Na+ with Mg2+ and Ca2+.
A scientometric study on quantitative microbial risk assessment in water quality analysis across 6 years (2016–2021)
Authors: Nyika Joan , Dinka Megersa 
Published: January 2022

Abstract
Water contamination by microbes is a growing environmental challenge that has exacerbated the apparent scarcity of safe drinking water. To alleviate this challenge, it is important to screen water for pathogens to reduce the risk of contracting waterborne diseases among consumers. The use of the quantitative microbial risk assessment (QMRA) tool to approximate illness possibility from exposure to microorganisms using dose–response models between pathogens and their associated health impacts is, therefore, recommended. The aim of this study was to explore the use of QMRA in water quality assessment using a scientometric approach and data sourced from the Web of Science (WoS) database for the period between 2016 and 2021. Articles were searched in the WoS databases before downloading the content for a bibliometric analysis using the VOSviewer software. Additionally, Microsoft Excel was used to analyze the inter-relationships of the searched results. Journal articles had the highest results from the searched query at 90.6% compared to other forms of output. High-impact journals such as the Science of the Total Environment and Water Research had the highest number of publications at 11.056 and 7.5%, respectively. Keyword analysis showed the multidisciplinary nature of the search query based on the clustered themes. The USA and China showed greater publication output with a total of 51.7% of total publications compared to developing countries due to their high research potential and extensive collaborative networks. A similar trend was evident in the institutional analysis with the University of California, USA, and the Chinese Academy of Sciences being the highest research contributors with 8% of the searched output. The study highlighted the need to extend research inputs to developing countries of Africa and Asia to improve their research and development capacity, productivity and share knowledge on QMRA in water quality assessment.
Environmental and social consequences for moving beyond archaic legislation and policy: delay and disjoint in water governance, Malawi
Authors: Chunga Brighton A. , Kusi-Appiah Andy , Masangano Clement , Mwamsamali Oswald K. 
Published: January 2022

Abstract
As in many sub-Saharan African countries with increasing demand for natural resources, how to move from archaic water legislation to new and up-to-date policies that cover both environmental and social consequences of water governance has become an issue. This study reviews Malawi's current governance framework and recommends a framework that takes Malawi's developmental needs into account. Our review of the state of water legislation shows that there is a lack of enforcement of the policies themselves and the public is not even aware of its existence. Lack of enforcement and public awareness have resulted in environmental degradation which is creating a lot of environmental problems for the citizens of Malawi. This work recommends future efforts in rationale evidence-based policy and legislation review that involves and is supported by multiple stakeholders. We also recommend the need for routine policy and legislation reviews in order to take advantage of cutting-edge solutions to water management issues. This will not only enhance general awareness of key environmental policies and legislation but benefit from coordinated efforts from various players to arrest the ongoing environmental degradation resulting from an incoherent policy and apathy from Malawians.
Understanding Engagement and the Potential Impact of an Electronic Drug Repository: Multi-Methods Study
Authors: Soobiah Charlene , Phung Michelle , Tadrous Mina , Jamieson Trevor , Bhatia R Sacha , Desveaux Laura 
Published: January 2022

Background
Centralized drug repositories can reduce adverse events and inappropriate prescriptions by enabling access to dispensed medication data at the point of care; however, how they achieve this goal is largely unknown.

Objective
This study aims to understand the perceived clinical value; the barriers to and enablers of adoption; and the clinician groups for which a provincial, centralized drug repository may provide the most benefit.

Methods
A mixed methods approach, including a web-based survey and semistructured interviews, was used. Participants were clinicians (eg, nurses, physicians, and pharmacists) in Ontario who were eligible to use the digital health drug repository (DHDR), irrespective of actual use. Survey data were ranked on a 7-point adjectival scale and analyzed using descriptive statistics, and interviews were analyzed using qualitative descriptions.

Results
Of the 161 survey respondents, only 40 (24.8%) actively used the DHDR. Perceptions of the utility of the DHDR were neutral (mean scores ranged from 4.11 to 4.76). Of the 75.2% (121/161) who did not use the DHDR, 97.5% (118/121) rated access to medication information (eg, dose, strength, and frequency) as important. Reasons for not using the DHDR included the cumbersome access process and the perception that available data were incomplete or inaccurate. Of the 33 interviews completed, 26 (79%) were active DHDR users. The DHDR was a satisfactory source of secondary information; however, the absence of medication instructions and prescribed medications (which were not dispensed) limited its ability to provide a comprehensive profile to meaningfully support clinical decision-making.

Conclusions
Digital drug repositories must be adjusted to align with the clinician’s needs to provide value. Ensuring integration with point-of-care systems, comprehensive clinical data, and streamlined onboarding processes would optimize clinically meaningful use. The electronic provision of accessible drug information to providers across health care settings has the potential to improve efficiency and reduce medication errors.
Analisis Kepuasan Kerja Pada Guru Di Smk Negeri 3 Singaraja (Sebuah Kajian Dari Perspektif Manajemen SDM)
Authors: Safhira Dea , Suarmanayasa I Nengah 
Published: January 2022

Abstract This study aims to: (1) describe the causes of low job satisfaction, (2) describe the impact of low job satisfaction, and (3) describe efforts to increase job satisfaction. The subjects of this research were all contract teachers at SMK Negeri 3 Singaraja, and the object of research was the low job satisfaction. The population in this study were all informants who would provide information about the low job satisfaction felt by contract teachers, consisting of the contract teachers themselves, the principal and students at SMK Negeri 3 Singaraja. This research is a qualitative descriptive study. This study uses purposive sampling and uses the Snowball technique which is useful for covering a wider sample as supporting data. The findings from the results of this study indicate that the causes of low job satisfaction experienced by contract teachers are due to several factors such as the lack of teaching hours for JTM contract teachers, delays in giving salaries, and teaching that is not in accordance with the competence of their skills.