bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transition Motion Synthesis for Object Interaction based on Learning Transition Strategies
Authors: Hwang Jaepyung , Park Gangrae , Kwon Taesoo , Ishii Shin 
Published: March 2022

AbstractIn this study, we focus on developing a motion synthesis framework that generates a natural transition motion between two different behaviours to interact with a moving object. Specifically, the proposed framework generates the transition motion, bridging from a locomotive behaviour to an object interaction behaviour. And, the transition motion should adapt to the spatio‐temporal variation of the target object in an online manner, so as to naturally connect the behaviours. To solve this issue, we propose a framework that combines a regression model and a transition motion planner. The neural network‐based regression model estimates the reference transition strategy to guide the reference pattern of the transitioning, adapted to the varying situation. The transition motion planner reconstructs the transition motion based on the reference pattern while considering dynamic constraints that avoid the footskate and interaction constraints. The proposed framework is validated to synthesize various transition motions while adapting to the spatio‐temporal variation of the object by using object grasping motion, and athletic motions in soccer.
Activation of P2X4 receptor exacerbates acute brain injury after intracerebral hemorrhage
Authors: Wu Si‐Ting , Han Jin‐Rui , Yao Nan , Li Yu‐Lin , Zhang Fang , Shi Yao , Shi Fu‐Dong , Li Zhi‐Guo 
Published: March 2022

AbstractIntroductionIntracerebral hemorrhage (ICH) accounts for 10%–15% of all strokes and culminates in high mortality and disability. After ICH, brain injury is initiated by the mass effect of hematoma, followed by secondary cytotoxic injury from dying brain cells, hematoma disintegration, and cascading brain immune response. However, the molecular mechanism of secondary cytotoxic brain injury in ICH is not completely understood. The sensitive purinergic receptor, P2X4 receptor (P2X4R), was known to recognize extracellular free ATP released by dying cells during tissue injury.AimsIn this study, we aim to understand the role of P2X4R in acute brain injury triggered by ICH.ResultsIn this study, we found that the sensitive purinergic receptor, P2X4R, was upregulated in the brain of patients with ICH as well as in a mouse model of ICH induced by collagenase injection. P2X4R blockage with the specific inhibitor 5‐BDBD attenuated brain injury in ICH mice by significantly reducing brain edema, blood–brain barrier leakage, neural death, and ultimately acute neurodeficits. Further study indicated that the protective effect of P2X4R inhibition is related to decreased pro‐inflammatory activity of microglia and recruitment of peripheral immune cells into the hemorrhagic brain.ConclusionsThese results suggest that the P2X4 receptor is activated by ICH stimuli which worsen brain injury following ICH.
Dnmt1 is required for the development of auditory organs via cell cycle arrest and Fgf signalling
Authors: Tang Dongmei , Zheng Shimei , Zheng Zhiwei , Liu Chang , Zhang Jiner , Yan Renchun , Wu Cheng , Zuo Na , Wu Lijuan , Xu Hongfei , Liu Shaofeng , He Yingzi 
Published: March 2022

AbstractObjectivesTo explore the role of DNA methyltransferase 1 (DNMT1) in the development of auditory system using zebrafish as experimental model.MethodsMorpholino oligonucleotide was used to induce Dnmt1 deficiency. RNA sequencing, in situ hybridization (ISH), whole genomic bisulfide sequencing (WGBS) and immunostaining were used to investigate the morphologic alterations and mechanisms.ResultsWe found that downregulation of Dnmt1 induced decreased number of neuromasts and repressed cell proliferation of primordium in the developing posterior lateral line system of zebrafish. The ISH data uncovered that Fgf signalling pathway was inhibited and the expression of chemokine memberscxcr4b,cxcr7bandcxcl12awere interfered, whilelef1expression was increased after inhibiting Dnmt1. Additionally, Dnmt1 downregulation led to malformed otoliths and deformed semicircular canals, and hair cell differentiation in utricle and saccule was inhibited severely. The in situ staining of otic placode markerspax2/5andfgf 3/8/10was decreased when Dnmt1 downregulated. The WGBS analysis demonstrated that the global methylation status was markedly downregulated, and cell cycle genes were among those most differently expressed between Dnmt1 morphants and the controls. Further ISH analysis confirmed the findings by RNA‐seq and WGBS assay thatcdkn1aandtp53were both upregulated after knockdown of Dnmt1.ConclusionOur results revealed that Dnmt1 is essential for the development of zebrafish auditory organ through regulating cell cycle genes together with Wnt and Fgf signalling pathways.
Neurostimulation in people with drug‐resistant epilepsy: Systematic review and meta‐analysis from the ILAE Surgical Therapies Commission
Authors: Touma Lahoud , Dansereau Bénédicte , Chan Alvin Y. , Jetté Nathalie , Kwon Churl‐Su , Braun Kees P. J. , Friedman Daniel , Jehi Lara , Rolston John D. , Vadera Sumeet , Wong‐Kisiel Lily C. , Englot Dario J. , Keezer Mark R. 
Published: March 2022

AbstractObjectiveSummarize the current evidence on efficacy and tolerability of vagus nerve stimulation (VNS), responsive neurostimulation (RNS), and deep brain stimulation (DBS) through a systematic review and meta‐analysis.MethodsWe followed the Preferred Reporting Items of Systematic reviews and Meta‐Analyses reporting standards and searched Ovid Medline, Ovid Embase, and the Cochrane Central Register of Controlled Trials. We included published randomized controlled trials (RCTs) and their corresponding open‐label extension studies, as well as prospective case series, with ≥20 participants (excluding studies limited to children). Our primary outcome was the mean (or median, when unavailable) percentage decrease in frequency, as compared to baseline, of all epileptic seizures at last follow‐up. Secondary outcomes included the proportion of treatment responders and proportion with seizure freedom.ResultsWe identified 30 eligible studies, six of which were RCTs. At long‐term follow‐up (mean 1.3 years), five observational studies for VNS reported a pooled mean percentage decrease in seizure frequency of 34.7% (95% confidence interval [CI]: −5.1, 74.5). In the open‐label extension studies for RNS, the median seizure reduction was 53%, 66%, and 75% at 2, 5, and 9 years of follow‐up, respectively. For DBS, the median reduction was 56%, 65%, and 75% at 2, 5, and 7 years, respectively. The proportion of individuals with seizure freedom at last follow‐up increased significantly over time for DBS and RNS, whereas a positive trend was observed for VNS. Quality of life was improved in all modalities. The most common complications included hoarseness, and cough and throat pain for VNS and implant site pain, headache, and dysesthesia for DBS and RNS.SignificanceNeurostimulation modalities are an effective treatment option for drug‐resistant epilepsy, with improving outcomes over time and few major complications. Seizure‐reduction rates among the three therapies were similar during the initial blinded phase. Recent long‐term follow‐up studies are encouraging for RNS and DBS but are lacking for VNS.
Effects of nucleos(t)ide analogs on hepatitis B surface antigen reduction with interferon‐lambda 3 induction in chronic hepatitis B patients
Authors: Umemura Machiko , Ogawa Koji , Morikawa Kenichi , Kubo Akinori , Tokuchi Yoshimasa , Yamada Ren , Kitagataya Takashi , Shigesawa Taku , Shimazaki Tomoe , Kimura Megumi , Suzuki Kazuharu , Nakamura Akihisa , Ohara Masatsugu , Kawagishi Naoki , Izumi Takaaki , Nakai Masato , Sho Takuya , Suda Goki , Natsuizaka Mitsuteru , Ono Kota , Murata Kazumoto , Sugiyama Masaya , Mizokami Masashi , Sakamoto Naoya 
Published: March 2022

AbstractBackground & AimsBenefits of nucleos(t)ide analogs (NAs) on hepatitis B surface antigen (HBsAg) reduction and interferon‐lambda3 (IFN‐λ3) induction are still not known. This study aimed to investigate the effects of NAs on HBsAg reduction and association with serum IFN‐λ3 levels in chronic hepatitis B (CHB) patients.MethodsA total of 91 patients [51 treated with nucleoside analog entecavir hydrate (ETV) and 40 treated with nucleotide analog adefovir dipivoxil (ADV) or tenofovir disoproxil fumarate (TDF)] with clinically evident CHB (chronic hepatitis, 57; liver cirrhosis, 34) were enrolled in this study. Serum IFN‐λ3 levels among patients receiving ETV and ADV/TDF were measured before the initiation of therapy and 1, 3, and 5 years post‐therapy.ResultsThe change (mean ± standard deviation) in serum HBsAg levels from baseline to year five was −0.38 ± 0.46 and −0.84 ± 0.64 log10 IU/ml in ETV and ADV/TDF groups, respectively (p = 0.0004). Higher serum IFN‐λ3 levels were observed in ADV/TDF group compared with ETV group during treatment (p < 0.001). Serum IFN‐λ3 levels showed negative correlation with HBsAg reduction in ADV/TDF group (r = −0.386, p = 0.038) at week 48. Nucleotide analogs (ADV/TDF) treatment has associated factors with −0.3 log HBsAg decline at 1 year, −0.5 log HBsAg decline at 3 years, and −0.8 log HBsAg decline at 5 years after NAs treatment on multivariate analysis.ConclusionsNucleotide analog (ADV/TDF) treatment reduced HBsAg levels greater compared with nucleoside analog (ETV) in parallel with IFN‐λ3 induction.
Santonian‐Campaniandinoflagellate cyst biostratigraphy and paleoenvironment of theKrishna‐GodavariBasin, India
Authors: Mishra Ashish K. , Singh Arun Deo , Prasad Vandana 
Published: March 2022

AbstractThe present study deals with the biostratigraphic framework of Late Cretaceous sediments of the subsurface Well (DNG) “A” (2085–1840 m depth) from the Raghavapuram/Chintalapalli Shale of the Krishna‐Godavari Basin and analyzed the palynological assemblage. The recorded palynological assemblage is dominated by marker dinoflagellate cyst viz.Areoligera coronata, Areoligera senonensis, Heterosphaeridium spinaconjunctum, Nelsoniella aceras, Nelsoniella semireticulata, Odontochitina porifera, Xenascus ceratioides, andXenascus gochtiispecies. The last occurrence (LO) of significant species of dinoflagellate cysts is considered for the biostratigraphic establishment. On the basis of dinoflagellate cyst biostratigraphy, the examined sequences correspond to the Santonian‐Campanian age. The dominance of gonyaulacoid dinoflagellate cysts is interpreted in terms of sea‐level fluctuation and other paleoenvironmental signals through this biostratigraphic establishment. The recorded dinoflagellate cysts indicate the outer neritic, cool depositional environment. The sea‐level rise, recorded in our study is linked with the transgressive phase during the Late Cretaceous (Santonian‐Campanian) period.
Vesicoureteral reflux treatment following kidney transplantation potentially prevents graft function deterioration and allows long‐term graft survival
Authors: Hirose Takayuki , Hotta Kiyohiko , Harada Hiroshi , Tanabe Tatsu , Sasaki Hajime , Shinohara Nobuo 
Published: March 2022

ObjectivesThe impact of vesicoureteral reflux post‐kidney transplantation on graft survival remains unclear, and guidelines on appropriate vesicoureteral reflux management post‐kidney transplantation are lacking. For this reason, we conducted a retrospective study on the impact of vesicoureteral reflux and its treatment on graft survival.MethodsWe evaluated 347 consecutive kidney transplantation recipients, who also underwent a ureteroneocystostomy, between 1996 and 2012.ResultsVesicoureteral reflux was diagnosed in 55 cases (15.9%), with a median post‐kidney transplantation duration of 50 months (range 0–172 months). Among these, 22 were monitored, 17 underwent transurethral collagen injections, and 16 received a ureteroneocystostomy. The 10‐year graft survival rate was significantly lower in recipients with vesicoureteral reflux (68.9%) than in those without vesicoureteral reflux (84.4%) (P = 0.0165). Moreover, among the vesicoureteral reflux recipients, the 10‐year graft survival rate was significantly higher in those whose vesicoureteral reflux was cured (80.1%) than in those whose vesicoureteral reflux persisted (53.6%) (P = 0.0062). Multivariate analysis showed that vesicoureteral reflux was significantly associated with both overall and death‐censored graft loss (odds ratio 3.737 and 3.685; P = 0.0015 and P = 0.0052, respectively). Lastly, the incidence of interstitial fibrosis and tubular atrophy was higher in recipients with vesicoureteral reflux than in those without vesicoureteral reflux (P = 0.0009).ConclusionsPost‐kidney transplantation vesicoureteral reflux has a negative impact on long‐term graft survival, and that treatment prevents graft deterioration. From the perspective of maintaining long‐term graft function in kidney recipients, vesicoureteral reflux may be one of the most important complications to be addressed.
Frailty in chronic kidney disease: challenges in nephrology practice. A review of current literature
Authors: Kennard Alice , Glasgow Nicholas , Rainsford Suzanne , Talaulikar Girish 
Published: March 2022

AbstractFrailty is a multidimensional clinical syndrome characterised by low physical activity, reduced strength, accumulation of multi‐organ deficits, decreased physiological reserve and vulnerability to stressors. Frailty pathogenesis and ‘inflammageing’ is augmented by uraemia, leading to a high prevalence of frailty potentially contributing to adverse outcomes in patients with advanced chronic kidney disease (CKD), including end‐stage kidney disease (ESKD). The presence of frailty is a stronger predictor of CKD outcomes than estimated glomerular filtration rate and more aligned with dialysis outcomes than age. Frailty assessment should form part of routine assessment of patients with CKD and inform key medical transitions. Frailty screening and interventions in CKD/ESKD should be a research priority.
Host range and virulence diversity of Pectobacterium carotovorum subsp. brasiliense strain RDKLR infecting radish in India, and development of a LAMP-based diagnostics
Authors: Chandrashekar Byalahalli Subramani , PrasannaKumar Mothukapalli Krishnareddy , Parivallal Perumal Buela , Pramesh Devanna , Banakar Sahana Nagaraj , Patil Swathi Shivanagouda , Mahesh Hirehally Basavarajegowda 
Published: March 2022

AbstractAimThis work aimed at determining the pathogenicity, molecular characterization, host range and rapid detection of Pectobacterium carotovorum subsp. brasiliense (Pcb) causing soft rot disease in radish.Methods and ResultsThe four isolated isolates were inoculated to radish, typical soft rot symptoms were observed and Koch’s postulates were proved. The most virulent strain RDKLR was morphologically and biochemically distinct. Pcb showed a positive potato soft rot test and elicited hypersensitivity response on Nicotiana tobaccum. The genes Pel2 and pmrA were used for subspecies characterization of Pcb. It has a wide host range and infection was observed on slices of carrot, tomato, radish, potato, cauliflower, cabbage, chilli, knol-khol, bell pepper and cucumber. Infectivity was also seen in seedlings under glasshouse conditions. Pcb produced cell wall degrading enzymes in semi-quantification assay and is a strong biofilm producer. The LAMP technique was standardized to help rapid detection and take prophylactic measures to manage the disease.ConclusionThis work reports Pcb as a new soft rot causing organism of radish in India. Pcb is highly virulent with a broad host range. The LAMP technique helps in rapid detection.Significance and impact of the studyPcb-induced soft rot causes significant yield loss, decreased market value, damage in transit, storage and the market. Disease characterization and early identification aid in disease management and prevention in the field.
Antrodia camphorata polysaccharide improves inflammatory response in liver injury via the ROS/TLR4/NF‐κB signal
Authors: Yang Yi , Han Chenyang , Sheng Yongjia , Wang Jin , Li Wenyan , Zhou Xiaohong , Ruan Shuiliang 
Published: March 2022

AbstractAntrodia Camphorata Polysaccharide (ACP) refers to a kind of polysaccharide extracted from the natural porous fungus Antrodia camphorata. This study investigated the mechanism of action of ACP in protecting the liver. The results showed that ACP suppressed the LPS‐induced KC cell activation, reduced the expression of inflammatory factors, increased the SOD level and suppressed ROS expression. In addition, N‐acetylcysteine (NAC) was adopted for pre‐treatment to suppress ROS. The results indicated that NAC synergistically exerted its effect with ACP, suggesting that ACP played its role through suppressing ROS. Further detection revealed that ACP activated the Nrf2 signal. It was discovered in the mouse model that, ACP effectively improved liver injury in mice, decreased ALT and AST levels, and suppressed the expression of inflammatory factors. This study suggests that ACP can exert its effect against oxidative stress via the Nrf2‐ARE signalling, which further improves the production of ROS and the activation of TLR4‐NF‐κB signalling, and protects the liver against liver injury.
Testing the polar auxin transport model with a selective plasma membrane H+‐ATPase inhibitor
Authors: Yang Yongqing , Liu Xiaohui , Guo Wei , Liu Wei , Shao Wei , Zhao Jun , Li Junhong , Dong Qing , Ma Liang , He Qun , Li Yingzhang , Han Jianyong , Lei Xiaoguang 
Published: March 2022

AbstractAuxin is unique among plant hormones in that its function requires polarized transport across plant cells. A chemiosmotic model was proposed to explain how polar auxin transport is derived by the H+ gradient across the plasma membrane (PM) established by PM H+‐adenosine triphosphatases (ATPases). However, a classical genetic approach by mutations in PM H+‐ATPase members did not result in the ablation of polar auxin distribution, possibly due to functional redundancy in this gene family. To confirm the crucial role of PM H+‐ATPases in the polar auxin transport model, we employed a chemical genetic approach. Through a chemical screen, we identified protonstatin‐1 (PS‐1), a selective small‐molecule inhibitor of PM H+‐ATPase activity that inhibits auxin transport. Assays with transgenic plants and yeast strains showed that the activity of PM H+‐ATPases affects auxin uptake as well as acropetal and basipetal polar auxin transport. We propose that PS‐1 can be used as a tool to interrogate the function of PM H+‐ATPases. Our results support the chemiosmotic model in which PM H+‐ATPase itself plays a fundamental role in polar auxin transport.
BAK1 plays contrasting roles in regulating abscisic acid‐induced stomatal closure and abscisic acid‐inhibited primary root growth in Arabidopsis
Authors: Deng Jinping , Kong Lingyao , Zhu Yinhua , Pei Dan , Chen Xuexue , Wang Yu , Qi Junsheng , Song Chunpeng , Yang Shuhua , Gong Zhizhong 
Published: March 2022

AbstractThe mechanisms that balance plant growth and stress responses are poorly understood, but they appear to involve abscisic acid (ABA) signaling mediated by protein kinases. Here, to explore these mechanisms, we examined the responses of Arabidopsis thaliana protein kinase mutants to ABA treatment. We found that mutants of BRASSINOSTEROID INSENSITIVE 1‐ASSOCIATED RECEPTOR KINASE 1 (BAK1) were hypersensitive to the effects of ABA on both seed germination and primary root growth. The kinase OPEN STOMATA 1 (OST1) was more highly activated by ABA in bak1 mutant than the wild type. BAK1 was not activated by ABA treatment in the dominant negative mutant abi1‐1 or the pyr1 pyl4 pyl5 pyl8 quadruple mutant, but it was more highly activated by this treatment in the abi1‐2 abi2‐2 hab1‐1 loss‐of‐function triple mutant than the wild type. BAK1 phosphorylates OST1 T146 and inhibits its activity. Genetic analyses suggested that BAK1 acts at or upstream of core components in the ABA signaling pathway, including PYLs, PP2Cs, and SnRK2s, during seed germination and primary root growth. Although the upstream brassinosteroid (BR) signaling components BAK1 and BR INSENSITIVE 1 (BRI1) positively regulate ABA‐induced stomatal closure, mutations affecting downstream components of BR signaling, including BRASSINOSTEROID‐SIGNALING KINASEs (BSKs) and BRASSINOSTEROID‐INSENSITIVE 2 (BIN2), did not affect ABA‐mediated stomatal movement. Thus, our study uncovered an important role of BAK1 in negatively regulating ABA signaling during seed germination and primary root growth, but positively modulating ABA‐induced stomatal closure, thus optimizing the plant growth under drought stress.
‘Paper care not patient care’: Nurse and patient experiences of comprehensive risk assessment and care plan documentation in hospital
Authors: Paterson Catherine , Roberts Cara , Bail Kasia 
Published: March 2022

AbstractAims and ObjectivesTo explore organisation‐wide experiences of person‐centred care and risk assessment practices using existing healthcare organisation documentation.BackgroundThere is increasing emphasis on multidimensional risk assessments during hospital admission. However, little is known about how nurses use multidimensional assessment documentation in clinical practice to address preventable harms and optimise person‐centred care.DesignA qualitative descriptive study reported according to COREQ.MethodsMetropolitan tertiary hospital and rehabilitation hospital servicing a population of 550,000. A sample of 111 participants (12 patients, 4 family members/carers, 94 nurses and 1 allied health professional) from a range of wards/clinical locations. Semi‐structured interviews and focus groups were conducted at two time points. The audio recording was transcribed, and an inductive thematic analysis was used to provide insight from multiple perspectives.ResultsThree main themes emerged: (1) ‘What works well in practice’ included: efficiency in the structure of the documentation; the Introduction, Situation, Background Assessment, Recommendation (ISBAR) framework and prompting for clinical decision‐making were valued by nurses; and direct patient care is always prioritised. (2) ‘What does not work well in practice’: obtaining the patient's signature on daily care plans; multidisciplinary (MDT) involvement; duplication of paperwork and person‐centred goals are not well‐captured in care plan documentation. (3) ‘Experience of care’; satisfaction of person‐centred care; communication in the MDT was important, but sometimes insufficient; patients had variable involvement in their daily care plan; and inadequate integration of care between MDT team which negatively impacted patients.ConclusionsEfficient and streamlined documentation systems should herald feedback from nurses to address their clinical workflow needs and can support, and capture, their decision‐making that enables partnership with patients to improve the individualisation of care provision.Relevance to clinical practiceThe integration of effective MDT involvement in clinical documentation was problematic and resulted in unmet supportive care from the patient's perspective.