bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fleeting: a Novel Ultrasonic Vocalization in Mice That Allows to Define Altered Developmental Milestones in Autism Spectrum Disorder
Authors: Ferreira Helena , Santos Sofia , Martins João , Castelo-Branco Miguel , Gonçalves Joana 
Published: January 2022

Abstract
A major mode of rodent communication occurs through ultrasonic vocalizations (USVs), which are influenced by environmental factors, mouse strain or genetic background and, importantly, by developmental stage. However, few studies have looked into the age-dependent evolution of spectral features of mouse USVs. Here, we report the existence of a novel vocalization, previously unreported, which we named “Fleeting” consisting of two acoustic elements produced with a narrow silent temporal interval between them. Strikingly, this vocalization pattern was extinguished after the second postnatal week, and this temporal pattern was associated with increased emission of Complex vocalizations, by gradual loss of the inter-element interval, suggesting a maturation process occurring at this time point. Importantly, the Fleeting vocalization was analyzed in a mouse model (Tsc2+/-) of Autism Spectrum Disorder (ASD), and showed an abnormal persistence, in particular in females which presented delayed conversion of Fleeting into Complex vocalizations compared with males. The identification of this novel vocalization represents an important insight into the maturation of mouse vocal repertoire and may be used as a developmental milestone in studies on neurodevelopmental disorders with communication impairments.
pH-Regulated Carbon Dots Fluorescence Sensor Array Detection of Multiple Heavy Metal Ions Under Stepwise Prediction
Authors: Xu Zijun , Liu Yuying , Chen Jiao , Wang Xiyuan , Liu Hao , Shi Qingdong , Wei Jianjie 
Published: January 2022

Abstract
As a large amount of heavy metals leaches into water sources from industrial effluents, heavy metal pollution has become an important factor affecting water quality. To enable the detection of multiple heavy metals, we constructed a pH-regulation fluorescence sensor array. Firstly, by adding a metal chelating agent as receptor, metal ions and carbon quantum dots (CDs) were connected to distinguish between Cr6+, Fe3+, Fe2+, and Hg2+ ions. Thus, the lack of affinity between the indicator functional groups and the analyte was solved. Secondly, by adjusting the pH environment of the solution system, an economical and simple array sensing platform is established, which effectively simplified the array construction. In this study, the SX-model was used in the field of fluorescence sensor array detection for metal ion recognition. Based on the strategy of stepwise prediction, combined with the classification and concentration models, the bottleneck of the unified model in previous studies was broken. This sensor array demonstrated sensitive detection of four heavy metal ions within a concentration range from 1 to 50 µM, with an accuracy of 95.45%. Moreover, it displayed the ability to efficiently identify binary mixed samples with an accuracy of 95.45%. Furthermore, metal ions in 15 real samples (lake water) were effectively discriminated with 100% accuracy. A chelating agent was used to improve the sensitivity of heavy metal ion detection and eventually led to high-precision prediction using the SX-model.
RNA Sequencing Analysis Between Ruptured and Un-Ruptured Brain AVM
Authors: Li Hao , Yan Zihan , Huo Ran , Ya Xiaolong , Xu Hongyuan , Jiao Yuming , Weng Jiancong , Wang Jie , Wang Shuo , cao yong 
Published: January 2022

Abstract
BackgroundBrain arteriovenous malformation (BAVM) arises as congenital vascular abnormalities with a significant risk for intracerebral hemorrhage (ICH). RNA sequencing technology has been recently used to investigate the mechanism of diseases owing to its ability to identify the gene changes on a transcriptome-wide level. In this study, we aimed to gain insights into the potential mechanism involved in BAVM rupture. MethodsSixty-five BAVM nidus samples were collected among which 28 were ruptured and 37 were un-ruptured. Then next-generation RNA sequencing were performed on all of them to obtain differential expressed genes (DEGs) between these two groups. In addition, bioinformatics analysis was performed to evaluate the involved biological processes and pathways by GO and KEGG analysis. Finally, univariate Cox regression analysis was utilized to obtain the early rupture-prone DEGs. Results: A total of 951 genes were differentially expressed between ruptured and un-ruptured BAVM group, of which 740 genes were up-regulated and 211 genes were down-regulated in ruptured BAVMs. Then bioinformatics analysis showed the biological processes and pathways related to the inflammatory processes and extracellular matrix organization were significantly enriched. Meanwhile, some of down-regulated genes are involved in cell adhesion and genes participating in response to muscle activity, as well as the terms about nervous system development. Finally, one hundred and twenty-five genes, a large number of which were involved in inflammation, were correlated with the early rupture of BAVMs. Conclusions: The up-regulated genes in ruptured BAVM group were involved in inflammatory processes and extracellular matrix organization while some of the down-regulated genes were participating in cell adhesion and myofibril assembly, indicating the role of enhanced inflammation and reduced vessel strength in BAVMs rupture.
Comparison of Natural and BNT162b2 Vaccine-induced Immunity, with and without an Enhancer or Booster Dose, on the Risk of COVID-19-Related Hospitalization in Israel
Authors: Waxman Jacob , Makov-Assif Maya , Reis Ben , Nezer Doron , Balicer Ran , Dagan Noa , Barda Noam 
Published: January 2022

Abstract
Introduction:
With the COVID-19 pandemic ongoing, accurate assessment of population immunity and the effectiveness of booster and enhancer vaccines is critical.
Methods
We compare COVID-19-related hospitalization incidence rate ratios, adjusted for potential demographic, clinical and health-seeking-behavior confounders, in 2,412,755 individuals (235,552,274 person-days), across four exposures: 2 BNT162b2 doses, 5 or more months prior ("non-recent vaccine immunity"); 3 BNT162b2 doses (“boosted vaccine immunity”); previous COVID-19, with or without a subsequent BNT162b2 dose (“natural immunity” and “enhanced natural immunity” respectively).
Results
Compared with non-recent vaccine immunity, COVID-19-related hospitalization incidence rate ratios are 11% (9%-13%) for boosted vaccine immunity, 34% (23%-50%) for natural immunity and 25% (17%-39%) for enhanced natural immunity.
Conclusion
We demonstrate that natural immunity (enhanced or not) provides better protection against COVID-19-related hospitalization than non-recent vaccine immunity, but less protection than that attained from booster vaccination. Additionally, our results suggest that vaccinating individuals with natural immunity further enhances their protection.
High Times and Dry Times In a Salt Marsh: Budgeting For Supplemental Freshwater Needs at the Big Boggy National Wildlife Refuge, USA
Authors: Madewell Matthew , Feagin Rusty , Huff Thomas , Balboa Bill 
Published: January 2022

Abstract
Salt marshes can be vulnerable to reduced freshwater input. Reduced freshwater inflows, particularly during a hot or dry summer season, can be catastrophic for vegetation productivity, organic accretion and inorganic sedimentation, and the ability of a marsh to maintain a sustainable elevation facing relative sea level rise (RSLR). Unfortunately, it is challenging for scientists to obtain inflow records for ungauged watersheds and link them with historical trends of salt marsh loss. We sought to address this challenge in Big Boggy National Wildlife Refuge (NWR), a small watershed in East Matagorda Bay, Texas. Our objective was to link the quantity of freshwater inflow with salt marsh sustainability and recommend management actions for the NWR. We first explored land cover trends and found that this watershed lost more than one-third of its low marsh since 1953. We then measured the streamflow into and out of the watershed, created a water budget, and modeled historical and future inflows from 1953 to 2100. Freshwater inflows have been increasing on average since 1953, but a combination of RSLR, sediment starvation, and punctuated seasonal droughts are likely responsible for the loss of salt marsh. We also estimated supplemental water needs during potential droughts out to 2100. We conclude that managers cannot fundamentally alter the accretion versus RSLR balance in this basin except by modifying freshwater input. Thus, during droughts, they should focus on providing these inputs and avoiding vegetation loss. Our work points to both water purchases and land management options that can achieve this goal.
Diatomite Modified With Alkyl Ketene Dimer For Hydrophobicity of Cellulosic Paper
Authors: Chen Zicheng , Fan Guangyuan , He Xiangyang , Xu Lei , Zhang Xuefeng , Zhang Lanhe 
Published: January 2022

Abstract
Multi-functionalization of papermaking chemicals is one of its main developing strategies. Fillers and internal sizing agents are often mutually restricted in practice. Therefore, it is feasible to prepare a new papermaking chemical by combining its functions. A process of diatomite modified with Alkyl ketene dimer (AKD) was developed in this study. The modified diatomite (AD) can concurrently play the role of mineral filler and sizing agent in the papermaking process. With the equal dosage of AKD, the AD had better sizing and retention performance than the commercial AKD emulsion in the case of cationic polyacrylamide (CPAM) and the CPAM/ bentonite retention system. The sizing mechanism of the AD can be interpreted as numerous hydrophobic sites and micro-surface structure of the paper sheet caused by the AD. Since the ester linkages were not detected in FT-IR spectra of the paper sheet filled by the AD, the chemical reaction may not be indispensable for its sizing performance. What’s more, an interesting “sticky” hydrophobicity phenomenon was observed when filling with AD. The approach in this study to prepare the “sticky” hydrophobic paper sheet can find its applications in some non-traditional application fields of cellulosic paper.
Prognostic Value of The Ratio of Maximum To Minimum Diameter of Primary Tumor In Metastatic Clear Cell Renal Cell Carcinoma
Authors: Shi Hongzhe , Cao Chuanzhen , Wen Li , Zhang Lianyu , Zhang Jin , Ma Jianhui , Shou Jianzhong , Li Changling 
Published: January 2022

Abstract
Background: Several models and markers were developed and found to predict outcome of advanced renal cell carcinoma. This study aimed to evaluate the prognostic value of the ratio of maximum to minimum tumor diameter (ROD) in metastatic clear cell renal cell carcinoma (mccRCC).Methods: Patients with mccRCC (n=213) treated with sunitinib from January 2008 to December 2018 were identified. Cut-off value for ROD was determined using receiver operating characteristic. Patients with different ROD scores were grouped and evaluated. Survival outcomes were estimated by Kaplan-Meier method.Results: The optimal ROD cutoff value of 1.34 was determined for progression free survival (PFS) and overall survival (OS). Patients in ROD≥1.34 group had shorter PFS (9.6 versus 17.7 months, p<0.001) and OS (25.5 versus 32.6 months, p<0.001) than patients in ROD<1.34 group. After adjustment for other factors, multivariate analysis showed ROD≥1.34 was an independent prognostic factor for PFS (p<0.001) and OS (p=0.006). Patients in ROD³1.34 group presented higher proportions of T3/4 stage (92.9% versus 7.1%, p=0.012), WHO/ISUP grade III/IV (72.0% versus 28.0%, p=0.010), tumor necrosis (71.0% versus 29.0%, p=0.039), sarcomatoid differentiation (79.1% versus 20.9%, p=0.007), poor MSKCC risk score (78.4% versus 21.6%, p<0.001) and poor IMDC risk score (74.4% versus 25.6%, p<0.001) than ROD<1.34 group.Conclusion: Primary tumor with higher ROD was an independently prognostic factor for both PFS and OS in patients with mccRCC who received targeted therapy. Higher ROD was also associated with high T stage, high WHO/ISUP grade, sarcomatoid features, tumor necrosis, poor MSKCC and IMDC risk score.
Analysis of the Efficacy of Metformin Against Doxorubicin Cardiotoxicity and Exploration of its Molecular Mechanism
Authors: Wei Jia , Wei Jin 
Published: January 2022

Abstract
Doxorubicin is a very effective broad-spectrum anti-tumor drug, but it can cause dose-dependent cardiotoxicity and ultimately lead to heart failure. Previous studies have found that metformin exerts a cardioprotective effect through AMP-activated protein kinase (AMPK), but its effect on doxorubicin cardiotoxicity is still unclear. In order to investigate whether and how AMPK affects the ability of metformin to regulate the cardiotoxicity of doxorubicin, we have conducted two parts: clinical research and basic research. We found that metformin can reduce doxorubicin cardiotoxicity through clinical retrospective case-control study. Based on this, animal experiments were conducted to explore the molecular mechanism, and it was found that metformin was not associated with AMPK pathway, an important pathway of energy metabolism in the body, and this pathway did not play a protective role in doxorubicin induced cardiotoxicity. The reason may be related to decreased glucose utilization and mitochondrial autophagy of cardiomyocytes.
Psychometric Validation of The Cognitive Abilities Screening Instrument Using Rasch Analysis In People With Dementia
Authors: Hsu Fang-Hsiao , Lee Ya-Chen , Chiu En-Chi 
Published: January 2022

Abstract
Background: The Cognitive Abilities Screening Instrument (CASI) is one of the most commonly used cognitive screening tools to assess overall cognitive function in people with dementia. However, the unidimensionliaty of the CASI using Rasch analysis has not been evaluated in people with dementia, limiting its utility in clinical and research settings. Unidimensionality verifies whether all items of a measure reflect a single theoretical construct, which is necessary to determine whether clinicians and researchers can appropriately use the sum scores of the CASI to describe overall cognitive function. This study aimed to examine unidimensionality of the CASI using Rasch analysis and estimate Rasch person reliability in people with dementia. Methods: CASI data of people with dementia was collected from medical records of one general hospital in northern Taiwan. A total of 506 people with dementia were recruited from the Department of Neurology. Unidimensionality was confirmed through two assumptions: (1) the infit and outfit mean square (MnSq) were 0.6-1.4, and (2) residual variance of the first principal component in principal component analysis was ≤ 20%. Rasch person reliability was estimated after undimensionality was supported. Results: One item from the list-generating fluency dimension was misfitted (outfit MnSq=1.42) and was deleted. The unidimensionality of the remaining 45 items (referred to as the CASI-45) was supported with an infit and outfit MnSq (0.85-1.24 and 0.84-1.28, respectively) and low residual variance of the first principal component (12.8%). The Rasch person reliability of the CASI-45 was 0.62. Conclusion: The CASI-45 showed a unidimensional construct and had acceptable Rasch person reliability in people with dementia.
Investigating Orthorexia Nervosa With The ORTO-R in a Sample of University Students With Or Without Subthreshold Autism Spectrum: Focus On Dietary Habits and Sex Differences
Authors: Dell’Osso Liliana , Cremone Ivan Mirko , Chiarantini Ilaria , Arone Alessandro , Casagrande Danila , Massimetti Gabriele , Carmassi Claudia , Carpita Barbara 
Published: January 2022

Abstract
Purpose: The aim of the present study was to investigate the presence of ON symptoms, measured by means of the ORTO-R, in a sample of University students with or without AT, specifically focusing on evaluating the role of sex and of dietary habits in the association between ON and autism spectrum. Methods: Subjects were requested to anonymously fulfil the ORTO-R and the Adult Autism Subthreshold Autism Spectrum (AdAS Spectrum) through an online form. Results: 2140 students participated in the study. Subjects with significant AT reported significantly higher ORTO-R scores than subjects without AT. Females and subjects following a vegetarian/vegan diet reported significantly higher ORTO-R scores than males and than subjects following an omnivorous diet, respectively. Significant positive correlations were found between ORTO-R and AdAS Spectrum scores. A decision tree model, with sex, type of diet and presence of AT as independent variables and ORTO-R score as dependent variable, showed in the first step the presence of significantly higher ORTO-R scores among females than among males, and in the second step showed in both sexes the presence of higher ORTO-R scores among subjects with AT than in those without. A significant interaction of sex and presence/absence of AT was reported on ORTO-R score, with a higher increasing trend of ORTO-R score with the increase of AdAS Spectrum score among females than among males. Conclusions: Our results further highlighted the association between AT and ON, in particular among females.
Probing Perovskite Carrier Dynamics under Sunlight
Authors: Li Bo-Han , Li Huang , Xuan Zhipeng , Zeng Wen , Wang Jia-Cheng , Zhou Chuanyao , Wang Xingan , Zhang Jingquan , Ren Zefeng , Yang Xueming 
Published: January 2022

Abstract
Understanding the nature of photogenerated carriers and their subsequent dynamics in perovskites is important for the development of related materials and devices. Most ultrafast dynamic measurements on the perovskite materials were conducted under high carrier densities, which likely obscures the genuine dynamics at low carrier densities under solar illumination conditions. In this study, we presented a detailed experimental study of the carrier density-dependent dynamics in hybrid lead iodide perovskites using a highly sensitive transient absorption spectrometer. We found that the carrier lifetime was about a hundred nanosecond in the linear response range, representing sunlight excitation, which was much longer than under high carrier densities. We also elucidated that the fast carrier decay (<1 ps) and the medium decay processes (tens of ps) occurred via the defect state trapping, and we determined its effects on the utilization percentage of photogenerated carriers through quantitative analysis. Furthermore, we obtained the Shockley-Queisser limit that took into account the carrier trapping effect, which directly reflected the material performance.
NEAT1 Enhances MPP+-Induced Pyroptosis In SH-SY5Y Cells Via Targeting Mir-5047/YAF2 Signaling
Authors: Shen Hong , Song Hui , Wang Songlin , Su Daojing , Sun Qiang 
Published: January 2022

Abstract
Parkinson’s disease (PD) is the second most frequent neurodegenerative disease. The aim of our study is to explore the role and regulatory mechanism of long non-coding RNA (lncRNA) NEAT1 in the MPP+-induced neuron pyroptosis. The levels of miR-5047 and YAF2 mRNA were determined through qRT-PCR. TUNEL staining was carried out to analyze neuronal apoptosis. Luciferase activity assay was accomplished to analyze the combination of miR-5047 and NEAT1 and YAF2 3’-UTR. Besides, the concentrations of IL-1β and IL-18 in supernatant were analyzed by ELISA assay. The levels of protein were examined through Western blot. NEAT1 and YAF2 expression were increased, while miR-5047 level was declined in the SH-SY5Y cells treated with MPP+. NEAT1 was a positively regulator for the SH-SY5Y cells pyroptosis induced by MPP+. In addition, YAF2 was a downstream target of miR-5047. NEAT1 promoted YAF2 expression via inhibiting miR-5047. Importantly, the promotion of NEAT1 to SH-SY5Y cells pyroptosis induced by MPP+ was recused by miR-5047 mimic transfection and YAF2 downregulation. In conclusion, NEAT1 was increased in the SH-SY5Y cells treated with MPP+, and it promoted the MPP+-induced pyroptosis through facilitating YAF2 expression by sponging miR-5047.
LAMTOR1 Phosphorylation of Exo70 Facilitates Tumor-Associated Macrophage Polarization to Restrain CD8+ T Cell Function in HCC
Authors: Wu Bo , Wang Qian , Li Bowen , Wang Xiaonan , Zhan Xiaoni , Shi Xiang , Wen Gehua , Wu Lingli , Liu Mei , Jiang Meixi 
Published: January 2022

Abstract
The tumor microenvironment controls the progression of tissue homeostasis leading to cancer.Accumulation of anti-inflammatory tumor-associated macrophages (TAM) has also been linked to worsening clinical outcomes as well as resistance to treatment in hepatocellular carcinoma(HCC).The current immune landscape for regulation by the presence of TAMs has been studies.It is known that LAMTOR1 undergoes phosphorylation to bind to Exo70 and other exocyst components and is enhancing the secretion of TGFB1 to facilitate the polarization of TAMs.The tumor-conditioned macrophages(TCM) numbers also correlated with enhanced number of regulatory T cells(Tregs) and decreased CD8+T cells in HCC.Mechanistically,TCM enhanced IL-10 production to diminished CD8+T cell activities.Our data demonstrate a novel immune therapeutic approach targeting TAMs immune suppression of T cell anti-tumor activities.
Posterior Laminectomy and Lateral Mass Screw Fixation for the Treatment of Severe Cervical Spondylotic Myelopathy
Authors: Li Chuang , Wu Bin , Wu Yongchao , Pan Haitao , Zheng Qixin 
Published: January 2022

Abstract
Background
Severe and complex cervical spondylotic myelopathy(CSM) requires surgical treatment. The common methods of posterior cervical spine surgery are laminoplasty and laminectomy with lateral mass screw internal fixation. However, the operative effect of this surgical approach is unclear owing to the complexity and severity of CSM in patients who undergo this surgical treatment.Therefore, we aimed to evaluate the clinical effects of posterior cervical laminectomy and lateral mass screw internal fixation in patients with severe and complex CSM.
Methods
We retrospectively analysed 60patients (48men,12women; mean age59.7 years) with severe and complex CSM who underwent posterior cervical laminectomy and lateral mass screw internal fixation from May 2013 to June 2020. Forty-eight patients underwent laminectomy and lateral mass screw internal fixation;12 patients underwent laminectomy and lateral mass screw internal fixation with 1–2 segmental laminoplasty. C-spine radiographs, computed tomography (CT) scans, and magnetic resonance imaging (MRI) were used to detect any curvature and fusion of the cervical spine, restenosis, and loose/broken internal fixation screws.Clinical efficacy was evaluated using Japanese Orthopaedic Association (JOA)scores, neck disability index(NDI), Odom’s classification, and the visual analogue scale(VAS) for pain.
Results
Overall effect was satisfactory at the last follow-up (average, 3.6 years [range, 6 months–6 years]), with no restenosis or loosened, slipped, or broken internal fixations. The anterior curvature angle of lordosis (n=46)was not significantly different from baseline at the last follow-up(P>0.05). The cervical kyphosis angle (n=14)was significantly improved at the last follow-up compared with the baseline value (P<0.05), as were the JOA, NDI, and VAS scores(all P<0.05). Odom’s classifications at the last follow-up were excellent, good, and fair in 45, 12, and three patients, respectively.
Conclusions
Posterior cervical laminectomy and lateral mass screw internal fixation achieved satisfactory clinical results in severe and complex CSM cases.Combining this surgical method with laminoplasty of 1–2 spinal segments stabilised the cervical spine and provided spinal decompression while preventing excessive backward drift of the cervical spinal cord, resulting in fewer complications.