bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxia-induced carbonic anhydrase mediated dorsal horn neuron activation and induction of neuropathic pain
Authors: Da Vitoria Lobo Marlene E. , Weir Nick , Hardowar Lydia , Al Ojaimi Yara , Madden Ryan , Gibson Alex , Bestall Samuel M. , Hirashima Masanori , Schaffer Chris B. , Donaldson Lucy F. , Bates David O. , Hulse Richard Philip 
Published: March 2022

Abstract
Neuropathic pain, such as that seen in diabetes mellitus, results in part from central sensitisation in the dorsal horn. However, the mechanisms responsible for such sensitisation remain unclear. There is evidence that disturbances in the integrity of the spinal vascular network can be causative factors in the development of neuropathic pain. Here we show that reduced blood flow and vascularity of the dorsal horn leads to the onset of neuropathic pain. Using rodent models (type 1 diabetes and an inducible endothelial-specific vascular endothelial growth factor receptor 2 knockout mouse) that result in degeneration of the endothelium in the dorsal horn, we show that spinal cord vasculopathy results in nociceptive behavioural hypersensitivity. This also results in increased hypoxia in dorsal horn neurons, depicted by increased expression of hypoxia markers such as hypoxia inducible factor 1α, glucose transporter 3, and carbonic anhydrase 7. Furthermore, inducing hypoxia through intrathecal delivery of dimethyloxalylglycine leads to the activation of dorsal horn neurons as well as mechanical and thermal hypersensitivity. This shows that hypoxic signalling induced by reduced vascularity results in increased hypersensitivity and pain. Inhibition of carbonic anhydrase activity, through intraperitoneal injection of acetazolamide, inhibited hypoxia-induced pain behaviours. This investigation demonstrates that induction of a hypoxic microenvironment in the dorsal horn, as occurs in diabetes, is an integral process by which neurons are activated to initiate neuropathic pain states. This leads to the conjecture that reversing hypoxia by improving spinal cord microvascular blood flow could reverse or prevent neuropathic pain.
Analysis of correlation factors influencing the outcome of initial 131I remnant ablative therapy in intermediate- to high-risk patients with papillary thyroid microcarcinoma
Authors: Wu Ruixin , Liu Wei , Li Ning , Wang Xuan , Sun Danyang , Ji Yanhui , Jia Qiang , Tan Jian , Zheng Wei 
Published: March 2022

Objective
To investigate the factors influencing the outcome of initial 131I remnant ablative therapy in intermediate- to high-risk patients with papillary thyroid microcarcinoma (PTMC).

Methods
We divided 99 patients with PTMC who underwent total thyroidectomy into two groups according to their response to initial 131I remnant ablative therapy: excellent response (ER) and non-ER groups. Clinical and laboratory characteristics were collected and retrospectively analyzed using univariate and multivariate binary logistic regression. Receiver operator characteristic (ROC) curves and diagnostic cutoff values were analyzed to evaluate the predictive value of significant quantitative influencing factors for 131I treatment outcomes. A prognostic nomogram model based on the above independent risk factors was established.

Results
Of the 99 eligible patients who accepted the initial 131I treatment following total thyroidectomy, 76 (76.7%) were classified into the ER group and 23 (23.3%) into the non-ER group. The univariate and multivariate analyses showed that extrathyroidal extension [ETE; odds ratio (OR) = 4.769; P = 0.041], preablative thyrotropin (TSH; OR = 0.972; P = 0.017), and stimulated thyroglobulin (sTg; OR = 1.614; P = 0.040) were independent predictors for the therapeutic effect of 131I treatment. Patients with higher sTg (>1.37 ng/ml) and lower TSH (<67.97 mU/l) and ETE tended to have a poor response to initial 131I treatment. The quantification of the therapeutic effect of initial 131I therapy in patients with PTMC using our newly constructed nomogram showed that ETE, preablative sTg, and TSH were contributors to non-ER.

Conclusion
Intermediate- to high-risk patients with PTMC after total thyroidectomy who had low pretreatment sTg and high preablative TSH levels and negative ETE were more likely to achieve satisfactory response to initial 131I remnant ablative therapy. Our prognostic nomogram is a valuable tool to enable patients and clinical professionals to be better informed about patients’ therapeutic response to initial 131I remnant ablative therapy.
Association Between Cardiovascular Disease and Cognitive Dysfunction in Breast Cancer Survivors
Authors: Von Ah Diane , Crouch Adele , Arthur Elizabeth , Yang Yesol , Nolan Timiya 
Published: March 2022

Background
Breast cancer survivors (BCSs) may have a greater risk for cardiovascular disease (congestive heart failure and hypertension), which in turn, can affect cognitive dysfunction, a frequent, bothersome, and potentially debilitating symptom.

Objective
The purpose of this study was to examine the relationship of cardiovascular disease on cognitive function in BCSs.

Methods
Baseline data from a double-blind randomized controlled trial for cognitive training of BCSs were examined. Early-stage BCS (stages I-IIIA) who were 21 years or older, completed adjuvant therapy (≥6 months), and reported cognitive concerns completed questionnaires and a brief neuropsychological assessment, including tests of memory, attention and working memory, speed of processing, and verbal fluency. Descriptive statistics, Pearson correlation coefficient, and separate linear regression models for each cognitive domain were conducted.

Results
Forty-seven BCSs, who were on average 57.3 (SD, 8.1) years old, were 58% White, and had some college education (75%), completed the study. Furthermore, 44.7% of the BCS had cardiovascular disease (congestive heart failure or hypertension). In linear regression models, cardiovascular disease was significantly related to immediate and delayed memory and attention and working memory (P < .01-.05).

Conclusion
Breast cancer survivors who have cardiovascular disease may also be at a greater risk for cognitive dysfunction post treatment. Results from this study inform both clinical practice and future research, specifically by examining the intersection between cancer, cardiovascular disease (cardiotoxicity), and cognition.

Implications for Practice
Nurses should be aware that BCSs with co-occurring cardiovascular disease are at a higher risk for cognitive dysfunction and work within the multidisciplinary team to optimize BCS health and function.
Elevated Expression of SATB1 Predicts Unfavorable Clinical Outcomes in Colon Adenocarcinoma
Authors: Li Yujiang , Liu Cuixia , Fu Yiwei , Zhai Hengyong , Chen Zhuyong , Yang Bin , Zhang Dongli 
Published: March 2022

Backgrounds:
Special AT-rich sequence-binding protein 1 (SATB1) belongs to the chromatin-remodeling protein which regulates different genes expression. High expression of SATB1 was found to be associated with the development of certain carcinomas. However, the functions of SATB1 in colon adenocarcinoma (CAC) remains unclear yet. Our study aims to investigate the potential role of SATB1 in CAC and whether it is associated with the unfavorable symptoms of CAC patients.

Methods:
The expression pattern of SATB1 was measured in CAC samples and adjacent noncancerous samples through quantitative real-time polymerase chain reaction and immunohistochemistry staining. We performed univariate and multivariate analyses to evaluate the clinical role of SATB1 in enrolled patients. The Kaplan-Meier analyses and log-rank tests were carried out to assess the clinicopathologic characteristics. The effect of SATB1 in human colon cancer cells was examined through cellular experiments.

Results:
The expression level of SATB1 in CAC tissues was significantly elevated compared with adjacent control tissues. High expression of SATB1 in tumor tissue was found to be associated with lymph node metastasis and advanced TNM stage. Higher SATB1 level in CAC patients indicated a worse 5-year survival time. Moreover, high SATB1 was defined as an independent poor prognostic factor. Cellular experiments showed that inhibition of the SATB1 protein level in human colon cells could suppress the migration and invasion capabilities.

Conclusions:
Our findings revealed that high expression of SATB1 was significantly correlated with the poor clinical features and prognosis of CAC patients. It indicated that SATB1 might serve as a potential prognostic predictor and novel drug target for CAC treatment.
QI Project Promoting NP Compliance with an AOM Bundle in Pediatric Hospital-owned Retail Clinic
Authors: Joo Kimberly R. , Sandberg Kelly , Albertini Bonnie , Sowar Lisa , Ziemnik Lisa S. 
Published: March 2022

Introduction:
The most common diagnosis for pediatric antibiotic prescriptions is acute otitis media (AOM). Therefore, it is logical to focus on AOM when striving to improve antibiotic prescribing in pediatrics. This quality improvement project aimed to improve documentation of diagnostic criteria and physical examination findings in the medical record and improve adherence to recommended antibiotic prescribing recommendations for AOM by nurse practitioners at a children’s hospital-owned pediatric retail clinic.

Methods:
We used The Institute for Healthcare Improvement’s Quality Improvement methodology to introduce an AOM quality bundle to providers in a pediatric retail clinic. We created Plan-Do-Study-Act ramps and implemented interventions, including NP education, electronic medical record improvements, and parent engagement. The percentage of compliant bundles (all five specific predetermined criteria successfully met) was measured for all patients diagnosed with AOM.

Results:
Baseline AOM bundle compliance began at a mean of 42%. Pareto analysis of baseline data indicated that antibiotic choice and duration were key failure modes. Antibiotic choice or duration errors occurred in 48% of reviewed charts at project inception. The interventions introduced throughout the project led to steady improvement in the percent of compliant bundles. The goal of 95% compliant bundles was achieved and maintained. At the project’s conclusion, 98% of antibiotic prescriptions were accurate.

Conclusions:
Implementation of multiple interventions with increasing levels of reliability improved the overall quality of documentation and increased the appropriate antibiotic prescriptions provided for patients diagnosed with AOM and seen by nurse practitioners at the retail clinic.
Implementation of a Provider’s Asthma-specific Note to Optimize Billing in the Pediatric Emergency Department
Authors: Hegamyer Emily N. , Zomorrodi Arezoo , Nelson Courtney E. 
Published: March 2022

Introduction:
There is broad variability in provider documentation for asthma encounters within the pediatric emergency department. Inadequate provider documentation leads to discrepancies between the ideal current procedural terminology (CPT) code and the assigned CPT code based on the care provided. Multiple studies demonstrate improvement in medical provider documentation after implementing standardized documentation templates and educational programs. The primary aim of this project was to improve the concordance between the ideal CPT code and assigned CPT code from a baseline of 71% to 85% in 12 months.

Methods:
We introduced an asthma-specific note template in January 2018. We reviewed a random sample of 20 encounters per month to compare the ideal and assigned CPT codes in the baseline and intervention periods. The primary outcome measure was the percentage of encounters with agreement between ideal and assigned billing. The secondary outcome measure was the percentage of encounters with intravenous magnesium that were billed for critical care. The process measure was asthma note usage. Provider education and Plan-Do-Study-Act (PDSA) cycles continued throughout the intervention period. We used statistical process control to measure changes over time.

Results:
We reviewed 740 patient encounters over a 12-month baseline and 25-month intervention period. The average agreement between ideal and assigned CPT code increased from 71% to 89%, with 84% usage of the asthma note template. The percentage of critical care billing for intravenous magnesium increased from 15% to 55%.

Conclusion:
Implementation of an asthma-specific provider note template in the pediatric emergency department improved billing optimization and critical care billing.
Improving Diagnostic Accuracy for Distinguishing Buckle Fractures From Other Distal Radius Fractures in Children
Authors: Ruess Lynne , Chmil Margarita , Singh Satbir , Samora Julie B. 
Published: March 2022

Introduction:
Accurately distinguishing between stable and unstable isolated distal radius fractures (DRF) in children allows for appropriate fracture-specific treatment. Although fractures with cortical disruption, displacement, or angulation are unstable, distinguishing stable buckle fractures (BF) from more subtle potentially unstable DRF is challenging. Our quality improvement project aimed to improve radiology reporting accuracy for these subtle fractures from 23% to 90% in a large tertiary pediatric hospital.

Methods:
Exams with a reported isolated distal radius fracture during baseline (January–March 2016) and intervention (April 2016–June 2019) were reviewed for accuracy. We introduced 3 types of interventions: radiologist education (self-directed learning modules and individual feedback), a new standardized report template, and a measurement tool (“The 1 cm Rule”). In addition, a statistical process control chart tracked accuracy data to study process changes over time.

Results:
During the baseline and intervention period, 22 and 480 radiographs, respectively, had either a stable BF or a potentially unstable isolated DRF. Each intervention type created a centerline shift. Overall, reporting accuracy increased from 23% to 90%. Most reports (95%, 639/676) used the template and standard terminology for reporting DRF.

Conclusions:
Radiology reporting diagnostic accuracy for distinguishing between stable BF and potentially unstable DRF in children increased to 90% through education, standardized reporting, and a measurement tool to enhance radiologist performance. Our institution plans to expand fracture-specific treatment practices with improved radiology reporting accuracy, including bracing and home management of stable BF diagnosed during an acute care visit.
Psychosocial Interventions for Patients With Heart Failure and Their Impact on Depression, Anxiety, Quality of Life, Morbidity, and Mortality: A Systematic Review and Meta-Analysis
Authors: Chernoff Robert Alan , Messineo Gabrielle , Kim Sungjin , Pizano Demetria , Korouri Samuel , Danovitch Itai , IsHak Waguih William 
Published: March 2022

ABSTRACT

Objective
The purpose of this systematic review and meta-analysis was to evaluate the ability of psychosocial interventions to reduce depression and anxiety, improve quality of life, and reduce hospitalization and mortality rates in patients with heart failure.

Methods
Studies of psychosocial interventions published from 1970 to 2021 were identified through four databases (PubMed, Ovid MEDLINE, PsycINFO, Cochrane). Two authors independently conducted a focused analysis and reached a final consensus on the studies to include, followed by a quality check by a third author. A risk of bias assessment was conducted.

Results
Twenty-three studies were identified, but only 15 studies of mostly randomized controlled trials with a total of 1370 patients with heart failure were included in the meta-analysis. Interventions were either cognitive behavioral therapy (CBT) or stress management. The pooled intervention effect was in favor of the intervention for depression (combined difference in standardized mean change [DSMC]: −0.41; 95% confidence interval [CI] = −0.66 to −0.17; p = .001) and anxiety (combined DSMC: −0.33; 95% CI = −0.51 to −0.15; p < .001) but was only a trend for quality of life (combined DSMC: 0.14; 95% CI = −0.00 to 0.29; p = .053). Evidence was limited that interventions produced lower rates of hospitalization (5 of 5 studies showing a beneficial effect) or death (1 of 5 with a beneficial effect).

Conclusions
CBT and stress management interventions significantly reduced depression and anxiety compared with control conditions. CBT significantly improved quality of life compared with controls, but stress management did not. Longer treatment duration seemed to be an important factor related to treatment success.
Unusual 68Ga-DOTATATE Uptake in Primary Nodal Hemangioma of Axillary Lymph Node
Authors: Sariyildiz Gumusgoz Hatice , Burak Zeynep , Karasah Erkek Burcin 
Published: March 2022

Abstract68Ga-DOTATATE PET/CT is a well-known modality in diagnosis, staging, and in predicting therapy response of neuroendocrine tumors. We present a case of 63-year-old woman with diagnosis of pancreas neuroendocrine tumor showing68Ga-DOTATATE uptake in a lymph node in left axilla. It was diagnosed as capillary hemangioma after biopsy.68Ga-DOTATATE uptake in bone hemangiomas is well-known; however, its uptake in a nodal hemangioma is worth to know as it might change the clinical perspective.
An Unusual False-Positive Uptake of Radioiodine Caused by Breast Implants
Authors: Wang Yu , Jiang Lisha , Xu Yangmengyuan , Zhang Xinyue , Liu Bin 
Published: March 2022

Abstract
A 40-year-old woman with a history of papillary thyroid cancer underwent ablative 131I therapy. Postablation whole-body 131I scintigraphy revealed not only increased activity in the thyroid bed but also in the anterior part of the chest. SPECT/CT images localized the activity in the bilateral breast implants.
High-density mapping of QTL controlling agronomically important traits in quinoa (Chenopodium quinoaWilld.)
Authors: Maldonado-Taipe Nathaly , Barbier Federico , Schmid Karl , Jung Christian , Emrani Nazgol 
Published: March 2022

AbstractQuinoa is a pseudocereal originating from the Andean regions. In spite of quinoa’s long cultivation history, genetic analysis of this crop is still in its infancy. We aimed to localize QTL contributing to the phenotypic variation of agronomically important traits. We crossed the Chilean accession PI-614889 and the Peruvian accession CHEN-109, which depicted significant differences in days to flowering, days to maturity, plant height, panicle length, thousand kernel weight (TKW), saponin content, and mildew susceptibility. We observed sizeable phenotypic variation across F2plants and F3families grown in the greenhouse and in the field, respectively. We used Skim-seq to genotype the F2population and constructed a high-density genetic map with 133,923 SNPs. Fifteen QTL were found for ten traits. Two significant QTL, common in F2and F3generations, depicted pleiotropy for days to flowering, plant height, and TKW. The pleiotropic QTL harbored several putative candidate genes involved in photoperiod response and flowering time regulation. This study presents the first high-density genetic map of quinoa that incorporates QTL for several important agronomical traits. The pleiotropic loci can facilitate marker assisted selection in quinoa breeding programs.Key messageSkim-sequencing enabled the construction a high-density genetic map (133,923 SNPs) and fifteen QTL were detected for ten agronomically important traits.
Fast Evolution of SOS-Independent Multi-Drug Resistance in Bacteria
Authors: Zhang Le , Guan Yunpeng , Cheng Yuen Yee , Cokcetin Nural N. , Bottomley Amy L. , Robinson Andrew , Harry Elizabeth J. , van Oijen Antoine , Su Qian Peter , Jin Dayong 
Published: March 2022

AbstractThe killing mechanism of many antibiotics involves the induction of DNA damage, either directly or indirectly, which triggers the SOS response. RecA, the master regulator of the SOS response, plays a crucial role in driving the evolution of resistance to fluoroquinolone antibiotics treated with a single dose of ciprofloxacin. However, the precise roles of RecA and SOS responses in the development of resistance under short-term β-lactam exposure remain unclear. In the present study, we observed a fast evolution of β-lactam resistance (20-fold increase in MIC in 8 hours) inE. coliafter deleting RecA and exposing the bacteria to a single dose of ampicillin. Notably, once this type of resistance is established, it remains stable and can be passed on to subsequent generations. Unlike earlier studies, we found that the rapid development of resistance relies on the hindrance of DNA repair, a mechanism that operates independently of the SOS response. Additionally, we identified the rapid emergence of drug resistance associated mutations in the resistant bacterial genome, indicating the impairment of DNA repair. Through comprehensive transcriptome sequencing, we discovered that the expression of numerous antioxidative response genes is repressed inrecAmutant resistant isolates, resulting in an excessive accumulation of ROS within the cells. This suggests that the induction of ROS drives the fast evolution of antibiotic resistance in RecA-deficient bacteria. Collectively, we show that the hindrance of DNA repair hampers cellular fitness, provides bacteria with genetic adaptability to survive in diverse stressful environments, and accelerates the evolution of antibiotic resistance.
TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thaliana
Authors: Stitz Michael , Kuster David , Reinert Maximilian , Schepetilnikov Mikhail , Berthet Béatrice , Janocha Denis , Artins Anthony , Boix Marc , Henriques Rossana , Pfeiffer Anne , Lohmann Jan , Gaquerel Emmanuel , Maizel Alexis 
Published: March 2022

AbstractPlants post-embryonic organogenesis requires matching the available metabolic resources to the developmental programs. The root system is determined by the formation of lateral roots (LR), which in Arabidopsis thaliana entails the auxin-induced activation of founder cells located in the pericycle. While the allocation of sugars to roots influences root branching, how sugar availability is sensed for auxin-triggered formation of LRs remains unknown. Here, we combine metabolic profiling with cell-specific genetic interference to show that LR formation is an important sink for carbohydrate accompanied by a switch to glycolysis. We show that the target-of-rapamycin (TOR) kinase is locally activated in the pericycle and the founder cells and that both chemical and genetic inhibition of TOR kinase lead to a block of LR initiation. TOR marginally affects the auxin-induced transcriptional response of the pericycle but modulates the translation of ARF19, ARF7 and LBD16, three key targets of auxin signalling. These data place TOR as a gatekeeper for post-embryonic LR formation that integrates local auxin-dependent pathways with systemic metabolic signals, modulating the translation of auxin induced gene expression.