bluuebunny/crossref_metadata_2025_split huggingface dataset:
DPYSL2/CRMP2isoform B knockout in human iPSC-derived glutamatergic neurons confirms its role in mTOR signaling and neurodevelopmental disorders
Authors: Feuer Kyra L. , Peng Xi , Yovo Christian , Avramopoulos Dimitri 
Published: November 2022

ABSTRACTDPYSL2/CRMP2is a microtubule-stabilizing protein crucial for neurogenesis and associated with numerous psychiatric and neurodegenerative disorders.DPYSL2has multiple RNA and protein isoforms, but few studies have differentiated between them or explored their individual functions. We previously demonstrated in HEK293 cells that a schizophrenia -associated variant in theDPYSL2B isoform (DPYSL2-B) reduced the length of cellular projections, created a transcriptomic disturbance that captured schizophrenia etiology, and was acted upon by the mTOR pathway. In the present study, we follow up on these results by creating, to our knowledge, the first models of endogenousDPYSL2-Bknockout in human induced pluripotent stem cells and excitatory glutamatergic neurons. We use CRISPR/Cas9 to specifically knock outDPYSL2-Band observe corresponding reduction of its RNA and protein. The average length of dendrites in knockout neurons was reduced up to 58% compared to controls. Transcriptome analysis reveals disruptions in pathways highly relevant to psychiatric disease including mTOR signaling, cytoskeletal dynamics, immune function, calcium signaling, and cholesterol biosynthesis. We also observed a significant enrichment of our differentially expressed genes in schizophrenia GWAS-associated loci. Our findings clarify the functions of the humanDPYSL2-Bisoform and confirm its involvement in molecular pathologies shared between many psychiatric diseases.
Bridging the light-electron resolution gap with correlative cryo-SRRF and dual-axis cryo-STEM tomography
Authors: Kirchweger Peter , Mullick Debakshi , Swain Prabhu Prasad , Wolf Sharon G. , Elbaum Michael 
Published: November 2022

AbstractCryo-electron tomography (cryo-ET) is the prime method for cell biological studies in three dimensions (3D) at high resolution. We have introduced cryo-scanning transmission electron tomography (CSTET), which can access larger 3D volumes, on the scale of 1 micron, making it ideal to study organelles and their interactionsin situ. Here we introduce two relevant advances: a) we demonstrate the utility of multi-color super-resolution radial fluctuation light microscopy under cryogenic conditions (cryo-SRRF), and b) we extend the use of deconvolution processing for dual-axis CSTET data. We show that cryo-SRRF nanoscopy is able to reach resolutions in the range of 100 nm, using commonly available fluorophores and a conventional widefield microscope for cryo-correlative light-electron microscopy (cryo-CLEM). Such resolution aids in precisely identifying regions of interest before tomographic acquisition and enhances precision in localizing features of interest within the 3D reconstruction. Dual-axis CSTET tilt series data and application of entropy regularized deconvolution during post-processing results in close-to isotropic resolution in the reconstruction without averaging. We show individual protein densities in a mitochondrion-ER contact in a cell region 850 nm thick. The integration of cryo-SRRF with deconvolved dual-axis CSTET provides a versatile workflow for studying unique objects in a cell.
Mortality associated with different influenza subtypes in France between 2015-2019
Authors: Goldstein Edward 
Published: November 2022

AbstractBackgroundHigh levels of excess mortality during periods of active influenza circulation in France were observed in the years preceding the COVID-19 pandemic. Some of the factors that affect the rates of influenza associated mortality are influenza vaccination coverage levels in different population groups and practices for testing for influenza and related use of antiviral medications for various illness episodes (including pneumonia hospitalizations) during periods of active influenza circulation in the community.MethodsData on sentinel ILI surveillance and sentinel virological surveillance in France were combined in a framework of a previously developed regression model to estimate the number of deaths associated with the circulation of the major influenza subtypes (A/H3N2, A/H1N1, B/Yamagata and B/Victoria) in France between 2015-2019.ResultsBetween week 3, 2015 and week 2, 2020, there were on average 15403 (95% CI (12591,18229)) annual influenza-associated deaths, of which 60.3% (49.9%,71.9%) were associated with influenza A/H3N2, and 29.5% (13.3%,45.5%) were associated with influenza B/Yamagata. During weeks when levels of ILI consultation in mainland France were above 50 per 100,000 persons, 7.9% (6.5%,9.4%) of all deaths in France were influenza-associated.ConclusionsHigh rates of influenza-associated mortality in France prior to the COVID-19 pandemic suggest that boosting influenza vaccination coverage in different population groups and testing for influenza in respiratory illness episodes (including pneumonia hospitalizations) during periods of active influenza (particularly influenza A/H3N2) circulation in combination with the use of antiviral medications is needed to mitigate the impact of influenza epidemics.
Impact of sustainable supply chain management on cost performance: empirical evidence from manufacturing companies of Bangladesh
Authors: Khanam Zobaida , Ghosh Ratan 
Published: November 2022

PurposeThe aim of the study has been performed to investigate the relationship between sustainable supply chain management (SSCM) practices and the cost performance of manufacturing firms in Bangladesh. Moreover, this paper highlights the key environment-friendly approaches and their association with financial performance in Bangladesh.Design/methodology/approachThe paper empirically assesses sustainable supply chain performance using four major supply chain practices, including sustainable procurement, sustainable production, sustainable distribution and investment recovery, and compares it with the cost performance. Twenty-four variables were identified through different literature and distributed as a structured questionnaire among the managers appointed in different manufacturing firms in Bangladesh. An empirical study was conducted using the Partial Least Square-Structural Equation Modeling (PLS-SEM) technique to examine the hypothesized relationships.FindingsThe results find a positive relationship in two variables of sustainable supply chain practices, including sustainable procurement and investment recovery, while sustainable distribution negatively impacted cost performance. In addition, sustainable production found no effect on cost performance.Research limitations/implicationsThe paper emphasizes the financial perspective of a sustainable supply chain without explicit consideration of sustainability's environmental and social dimensions.Practical implicationsThis study has implications for the literature on the SSCM approaches of manufacturing firms in the least developed economies. In addition, this study could work as a guideline for some manufacturing industries that prefer a policy or standard to alter their traditional supply chain management system to a sustainable supply chain.Originality/valueThe paper provides a comprehensive framework for evaluating the coordinated effect of SSCM practices on cost performance where variables of four specific activities of SSCM and cost performance are adopted from different studies. Further studies could be initiated, including some other eco-friendly supply chain variables, and the effect could be evaluated from an environmental perspective.
Marketing and performance in small firms: the role of networking
Authors: McCartan Aodheen 
Published: November 2022

Purpose
The purpose of the paper is to advance knowledge of small firm performance by explicating how networking helps small firms carry out marketing and perform better.

Design/methodology/approach
An online survey of small firm owner-managers in a regional economy of the UK was executed. Hypotheses were tested including the proposition that networking proactiveness moderates the relationship between market orientation (MO) and performance.

Findings
It was found that networking is undertaken by all small firms across a spectrum of business types. Networking is seen as applicable, it results in valuable outcomes and these outcomes contribute to marketing. The contribution increases with firm size and is valued more in small firms with a dedicated marketing function. Proactive networking creates greater value than reactive networking, but proactive networking in and of itself does not lead to greater performance. Rather, networking proactiveness moderates the relationship between MO and performance.

Practical implications
It is argued that networking should not be dismissed as “not quite proper” marketing and should be harnessed as a way of compensating for marketing activities that are outside the reach of the small firm. Small firms are advised to adopt a proactive approach to their networking activities, as without a reasonable level of proactiveness, there is likely to be no benefit in being market oriented.

Originality/value
There has been little research that has examined the specific ways in which networking contributes to marketing and none that probes if and to what extent this contribution makes a difference to overall firm performance. This paper addresses this gap.
Interactions between protea plants and their animal mutualists and antagonists are structured more by energetic than morphological trait matching
Authors: Neu Alexander , Cooksley Huw , Esler Karen J. , Pauw Anton , Roets Francois , Schurr Frank M. , Schleuning Matthias 
Published: November 2022

Abstract

Traits mediate mutualistic and antagonistic interactions between plants and animals, and should thus be useful for predicting trophic species interactions. Studies to date have examined the importance of morphological trait matching for plant–animal interactions, but have rarely explored the extent to which these interactions are shaped by matching between energetic provisions of plants and energetic demands of animals.

We tested whether energetic and/or morphological trait matching shapes interactions between Protea plant species and their interacting animal mutualists and antagonists in the Cape Floristic Region, South Africa.

We recorded interactions between 22 Protea species, pollinating insects and vertebrates as well as seed predators (endophagous insect larvae in protea cones) at 21 study sites. To relate species interactions to matching trait pairs, we measured key morphological traits (shape and size of flower heads and seed cones, and mouth part length as well as body length) and quantified the animals' energetic demands (metabolic rate) together with the plants' energetic provisions (nectar sugar amount, seed‐to‐cone mass ratio). We calculated log ratios of both energetic and morphological traits between animals and plants as predictor variables for the number of observed interactions between Protea species and their animal interaction partners.

For both mutualistic and antagonistic interactions, we found significant effects of morphological and energetic trait ratios on the interactions between plants and animals. Trait ratios accounted for 11% to 22% of variation in species interactions. Consistent with energetic trait matching, we found a hump‐shaped relationship between interaction frequency and log ratios of energetic traits of animals and plants, indicating that interactions were most frequent at intermediate log ratios between energetic demand and provision. Effects of morphological trait ratios on interactions were statistically supported in most cases, but were variable in the magnitude and shape of the predicted relationships.

Across animal taxa and interaction types, energetic traits had more consistent effects on interactions between plants and animals than morphological traits. This suggests that energy can function as an important interaction currency and facilitate the understanding and prediction of trophic species interactions.

Read the free Plain Language Summary for this article on the Journal blog.
Keeping connected: Family therapists' experiences of working online during the COVID‐19 pandemic
Authors: Beet Naomi , Ademosu Temitope 
Published: November 2022

AbstractFor this qualitative study, eight family therapists were interviewed about their experiences of practising online during the pandemic. Findings are organised using a framework of problems, possibilities, resources and restraints (PPRR, Neden & Burnham, 2007). Despite variation in therapist confidence in online practice, all participants found new possibilities in this way of working, including connecting family members across distance, increased co‐construction within therapeutic relationships and engaging clients who would not usually attend appointments. Therapeutic alliance was generally possible to establish online, though felt more challenging with whole families than individuals. Problems and restraints included therapist fatigue, risk and safety management, and attuning to nuanced expression of emotion. Implications for practice and future research are proposed. It is suggested that systemic practice has unique qualities to offer the field of online psychotherapy.
Effects of 3,4‐methylenedioxymethamphetamine and methamphetamine on motor vehicle driving performance: A systematic review of experimental and observational studies
Authors: McGrane Ian R. , Ramsbacher Nathan C. , Rook William C. , Omar Faddy A. 
Published: November 2022

AbstractMethamphetamine (METH) and 3,4‐methylenedioxymethamphetamine (MDMA) are common drugs of abuse and driving under their influence may occur in 1 million people yearly in the United States. This systematic review fills the currently unmet need in understanding the effects of METH and MDMA on motor vehicle driving performance (MVP) and provides insight into the forensic community. A PubMed search on September 24, 2020, for experimental and observational studies, which evaluated the impact of METH and MDMA on MVP was performed. After a review of 208 abstracts, 103 were considered potentially interesting and full texts were obtained. After the exclusion of non‐English articles, review articles, single case reports, and articles which did not evaluate METH or MDMA on MVP, a total of nine experimental studies, 10 traditional observational studies, and 35 case series were included. The clinical rigor of experimental studies was evaluated using the Jadad scale. Experimental studies often demonstrated no significant MVP safety signals for METH or MDMA use, which was contrary to the overwhelming MVP safety risks found in observational studies. Common driving behaviors while using METH or MDMA include: errors in judgment, traveling at high speeds, failure to stop, merging inappropriately, lane weaving, and crashes. Limitations of experimental studies that led to dissimilar MVP outcomes from observational studies include: the common use of driving simulators, as opposed to actual driving examinations, and doses of METH or MDMA administered may not be representative of blood concentrations seen in observational studies. This systematic review has no funding source and was not registered.
Emoji meanings (pleasure–arousal–dominance dimensions) in consumer research: Between‐country and interpersonal differences
Authors: Schouteten Joachim J. , Llobell Fabien , Chheang Sok L. , Jin David , Jaeger Sara R. 
Published: November 2022

AbstractIn line with the increasing popularity of emoji, the need for methodological research into these pictorial representations of emotion remains. The present research contributes to this goal by continuing to establish the meaning of emoji and exploring these according to between‐country and interpersonal differences. The emoji (n = 12) were selected to span the valence × arousal emotion space, and the PAD model (Pleasure–Arousal–Dominance) was used to establish emoji meaning for the three dimensions, operationalized as measurement on 6 × 3 semantic differentials. Participants in the main study came from three countries—Germany, Singapore, and Malaysia (n = 2465), and a supplementary study included the United Kingdom and New Zealand (n = 600) (subset of four emoji). The results confirmed that emoji meanings according to the PAD model were largely similar between countries (albeit not identical). There were multiple minor significant differences for individual emoji, and where these existed, they often related to the dimension of Arousal, prompting a need for further investigation. Interpersonal differences were examined for gender (men and women), age group (18–45 and 46–69 years old), and frequency of emoji use. Again, significant differences were smaller rather than larger and supported the notion that emoji are generally applicable for multicountry research. However, caution regarding the participants who use emoji infrequently may be warranted.Practical ApplicationThe findings from this research will help academics and practitioners who are interested in using emoji for sensory and consumer research (or are already doing so) with more robust interpretations of their findings. For a set of 12 emoji that provide broad coverage of the valence × arousal emotional space, meanings are provided on the three dimensions of the PAD model. The data is collected in five countries and contributes to increased confidence that emoji meanings are by and large similar in these countries.
Haplotype‐phased and chromosome‐level genome assembly of Puccinia polysora, a giga‐scale fungal pathogen causing southern corn rust
Authors: Liang Junmin , Li Yuanjie , Dodds Peter N. , Figueroa Melania , Sperschneider Jana , Han Shiling , Tsui Clement K. M. , Zhang Keyu , Li Leifu , Ma Zhanhong , Cai Lei 
Published: November 2022

AbstractRust fungi are characterized by large genomes with high repeat content and have two haploid nuclei in most life stages, which makes achieving high‐quality genome assemblies challenging. Here, we described a pipeline using HiFi reads and Hi‐C data to assemble a gigabase‐sized fungal pathogen, Puccinia polysora f.sp. zeae, to haplotype‐phased and chromosome‐scale. The final assembled genome is 1.71 Gbp, with ~850 Mbp and 18 chromosomes in each haplotype, being currently one of the two giga‐scale fungi assembled to chromosome level. Transcript‐based annotation identified 47,512 genes for the dikaryotic genome with a similar number for each haplotype. A high level of interhaplotype variation was found with 10% haplotype‐specific BUSCO genes, 5.8 SNPs/kbp, and structural variation accounting for 3% of the genome size. The P. polysora genome displayed over 85% repeat contents, with genome‐size expansion and copy number increasing of species‐specific orthogroups. Interestingly, these features did not affect overall synteny with other Puccinia species having smaller genomes. Fine‐time‐point transcriptomics revealed seven clusters of coexpressed secreted proteins that are conserved between two haplotypes. The fact that candidate effectors interspersed with all genes indicated the absence of a “two‐speed genome” evolution in P. polysora. Genome resequencing of 79 additional isolates revealed a clonal population structure of P. polysora in China with low geographic differentiation. Nevertheless, a minor population differentiated from the major population by having mutations on secreted proteins including AvrRppC, indicating the ongoing virulence to evade recognition by RppC, a major resistance gene in Chinese corn cultivars. The high‐quality assembly provides valuable genomic resources for future studies on disease management and the evolution of P. polysora.
Cysteine restriction‐specific effects of sulfur amino acid restriction on lipid metabolism
Authors: Nichenametla Sailendra N. , Mattocks Dwight A. L. , Cooke Diana , Midya Vishal , Malloy Virginia L. , Mansilla Wilfredo , Øvrebø Bente , Turner Cheryl , Bastani Nasser E. , Sokolová Jitka , Pavlíková Markéta , Richie John P. , Shoveller Anna K. , Refsum Helga , Olsen Thomas , Vinknes Kathrine J. , Kožich Viktor , Ables Gene P. 
Published: November 2022

AbstractDecreasing the dietary intake of methionine exerts robust anti‐adiposity effects in rodents but modest effects in humans. Since cysteine can be synthesized from methionine, animal diets are formulated by decreasing methionine and eliminating cysteine. Such diets exert both methionine restriction (MR) and cysteine restriction (CR), that is, sulfur amino acid restriction (SAAR). Contrarily, SAAR diets formulated for human consumption included cysteine, and thus might have exerted only MR. Epidemiological studies positively correlate body adiposity with plasma cysteine but not methionine, suggesting that CR, but not MR, is responsible for the anti‐adiposity effects of SAAR. Whether this is true, and, if so, the underlying mechanisms are unknown. Using methionine‐ and cysteine‐titrated diets, we demonstrate that the anti‐adiposity effects of SAAR are due to CR. Data indicate that CR increases serinogenesis (serine biosynthesis from non‐glucose substrates) by diverting substrates from glyceroneogenesis, which is essential for fatty acid reesterification and triglyceride synthesis. Molecular data suggest that CR depletes hepatic glutathione and induces Nrf2 and its downstream targets Phgdh (the serine biosynthetic enzyme) and Pepck‐M. In mice, the magnitude of SAAR‐induced changes in molecular markers depended on dietary fat concentration (60% fat >10% fat), sex (males > females), and age‐at‐onset (young > adult). Our findings are translationally relevant as we found negative and positive correlations of plasma serine and cysteine, respectively, with triglycerides and metabolic syndrome criteria in a cross‐sectional epidemiological study. Controlled feeding of low‐SAA, high‐polyunsaturated fatty acid diets increased plasma serine in humans. Serinogenesis might be a target for treating hypertriglyceridemia.