bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of β3 -adrenoceptor Activation on interaction of Renal Adrenoceptors and Angiotensin II Receptors in Aging ApoE-/- mice
Authors: Wang Wei , Li Fengde , Li Yanfang , Jiang Zhili , Song Junying 
Published: June 2022

Abstract
To investigate the effect of β3-adrenoceptor (β3-AR) activation on the interaction of renal adrenoceptor (AR) and angiotensin II receptor (ATR) subtypes in aging apolipoprotein E knockout (apoE-/-) mice. 10 C57BL/6J mice served as normal control group (group A) and 50 age-matched apoE-/- mice randomly divided into hyperlipidaemia model group (group B), atorvastatin treatment group (group C), low-dose β3-AR agonist group (group D), high-dose β3-AR agonist group (group E) and β3-AR antagonist group (group F) were fed with high fat diet for 38 weeks, treatment in each group was given during the last 12 weeks of the high-fat diet. Then serum non-high density lipoprotein cholesterol (nHDL-C), blood glucose and insulin levels in each group were measured using an automatic biochemical detector. Expression of α1A-AR, α1B-AR, α2A-AR, β1-AR, β2-AR, β3-AR and angiotensin II type 1 (AT1R) and type 2 (AT2R) receptors in renal tissues were detected by RT-PCR and Western blot, respectively. Results showed that the serum nHDL-C, blood glucose and insulin levels significantly increased in hyperlipidaemia model mice (P<0.01), and the expression of α1A-AR, AT1R and AT2R in renal tissues were significantly upregulated (P<0.01), with the expression of α1B-AR, α2A-AR, β1-AR, β2-AR and β3-AR downregulated (P<0.01). Compared with group B, nHDL-C, glucose and insulin in aged apoE-/- mice decreased in group D and E (P<0.01). β3-AR agonist treatment could down-regulate the expression of α1A-AR and AT1R (P<0.05), and increased α1B-AR, α2A-AR, β1-AR, β3-AR and AT2R expression in group D and E (P<0.05), and thus resulted in improved lipid and glucose metabolism in aging hyperlipidaemia ApoE-/- mice, indicating that there are interaction between β3- AR and different subtypes of AR, and between β3-AR and ATRs, which may play a protective role in renal function injury caused by hyperlipidemia.
Single Nucleotide Polymorphism in Deoxyribonuclease I (DNase I, Q222R/rs1053874 A/G) is associated with Systemic Lupus Erythematosus (SLE)
Authors: Zedan Mohamed M. , Mutawi Thuraya M. , Salah Mona T. , Attia Zeinab R. 
Published: June 2022

Abstract
Deoxyribonuclease I (DNase I) is a potential endonuclease enzyme implicated in several immune diseases such as SLE. The objective of this study was to investigate the influence of rs1053874 A/G DNase I polymorphism on SLE susceptibility and to study its influence on serum DNase I levels. The genomic DNA for all participants was genotyped utilizing TaqMan™ assay and Serum DNase I levels were determined by enzyme-linked immunosorbent (ELISA) assay. Individuals with the GA, AA, GA + AA genotypes, and A allele of rs1053874 A/G in the DNase I gene had decreased SLE risk. Also, regression analysis revealed that patients with the genotype "GA + AA" and decreased DNase I levels had decreased risk for prediction of SLE activity (P < 0.05). In Conclusion, The DNase I rs1053874 A/G reduced the incidence of SLE in the Egyptian population. Moreover, “GA + AA” in the same SNP is thought to be an independent predictor of the progression of SLE by reducing serum DNase I levels.
Budd–Chiari syndrome versus Pyrrolizidine alkaloids-induced hepatic sinusoidal obstructive syndrome: A multi-center study
Authors: Tong Yaru , Zhuge Yuzheng , Zhang Ming , Qi Zexue , Wu Wei , Chen Jinjun , He Fuliang , Zhang Xiaofeng , Zhang Feng , Li Taishun , Zhang Bin , Zhang Wei , Yin Qin , Ding Pengxu , Wang Guangchuan 
Published: June 2022

Abstract
Background
Budd-Chiari syndrome (BCS), especially BCS-hepatic vein occlusion type (BCS-HV), and Pyrrolizidine alkaloids-induced hepatic sinusoidal obstructive syndrome (PA-HSOS) share many clinical characteristics. In this study, we comprehensively compare the two diseases to identify key characteristics.
Methods
139 patients with BCS-HV and 257 PA-HSOS patients admitted to six hospitals between January 2014 and October 2021 were retrospectively analyzed. The two groups were then comprehensively compared and analyzed clinically.
Results
Among the 139 BCS-HV and 257 PA-HSOS patients, the mean age and the median time from symptom onset to the first visit have a statistical difference. 257 PA-HSOS patients all had a history of taking PA-containing plants, while no patients in the BCS-HV group were exposed to PA-containing plants (p < 0.05). BCS-HV and PA-HSOS were found in abdominal distension, abdominal pain, ascites, abdominal wall varicose veins and jaundice were significantly different (p < 0.05). The prevalence of local stenosis or occlusion of the hepatic vein in BCS-HV group was higher than that in the PA-HSOS group (P < 0.05). On the three characteristics of hepatic vein traffic branches, enlarged caudate lobe of the liver and early liver enhancement nodules, the prevalence in BCS-HV was 73.90%, 47.70%, and 8.50%, while those of the PA-HSOS group were zero (P < 0.05).
Conclusion
The history of PA intake, local stenosis or occlusion of the hepatic vein, a communicating branch of the hepatic vein, and caudate lobe hypertrophy are the key factors to distinguish the two diseases.
Novel vitamin K3 analogues containing 3-N-substituted aromatic and piperazine rings with selective in vitro anticancer activity against HeLa, U87 MG, and MCF-7
Authors: Abdassalam Aesha FSH. , Deniz Nahide Gulsah , SAYIL CIGDEM , Onay-Ucar Evren , Mertoglu Elif , Arda Nazli 
Published: June 2022

Abstract
In this study, firstly, five novel vitamin K3 analogues were synthesized by the reactions of vitamin K3 (2-methyl-1,4-naphthoquinone, also known as menadione) with some aromatic and heterocyclic ring substituted nucleophiles such as 2,4-dimethoxyaniline, 4-methoxyaniline, 4-benzylpiperidine and 1-(2-aminoethyl)piperazine in ethanol/Na2CO3, and 1-(diphenylmethyl)piperazine in chloroform/triethylamine (TEA) at room temperature. Their structures were elucidated by Fourier transform infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (1H NMR), attached proton test nuclear magnetic resonance (APT-NMR) and mass spectrometry (MS). Secondly, in vitro cytotoxic effects of vitamin K3 analogues were investigated by MTT assay against three cancer cell lines (HeLa, U87 MG, and MCF-7) to evaluate their anticancer activity and a human embryonic kidney cell line (HEK-293) to check their cancer cell selectivity. One of the compounds, namely 2-((2,4-dimethoxyphenyl)amino)-3-methylnaphthalene-1,4-dione, was found to inhibit the growth of HeLa cervical cancer cells selectively, even better than vitamin K3, at a non-toxic concentration for healthy cells. The selectivity index of this compound for HeLa cells was calculated approximately as 3. Vitamin K3 was more effective against U87 MG and MCF-7 cells than its derivatives, moreover it was the only compound, which was significantly toxic to breast cancer cells, but its selectivity was poor. Furthermore, anticancer properties of piperazine derivatives of vitamin K3 were investigated by us for the first time in this study.
Image Segmentation Based on GOA for Finding Deformities in Medical and Aura Images
Authors: poojary manjula , Yarramalle Srinivas 
Published: June 2022

Abstract
Image segmentation is one of the challenging task in the area of digital image processing. Many methods are available for undergoing segmentation among which clustering techniques are assumed to be more outstanding. In this article the Expectation Maximization (EM) based Gaussian Mixture Model (GMM) is considered for segmentation. In order to identify the feature this article proposes a new Grasshopper Optimization Algorithm (GOA) based approach for segmentation along with BGMM and EM algorithm. The results are carried out on benchmark dataset namely UCI for medical images and aura images from Bio-well data sets. The results are evaluated using image quality metrics.
Clinicopathological significances and prognostic value of PPFIA4 in colorectal cancer
Authors: Fu Fangmei , Zheng Minying , Yang Xiaohui , Fan Linlin , Chen Shuo , Gao Ming , Zhang Shiwu 
Published: June 2022

Abstract
Purpose
The PPFIA gene family (PPFIA1, PPFIA2, PPFIA3, and PPFIA4) is associated with multiple human diseases, particularly malignant tumors. However, the expression and prognostic value of the PPFIA family in human colorectal cancers (CRCs) have not been reported.
Materials and methods
In this study, several databases, including Oncomine, UALCAN, and the cancer cell line encyclopedia, were used to compare differences in PPFIA1, PPFIA2, PPFIA3, and PPFIA4 expression between normal colon samples and CRCs. The expression levels of these four proteins were used to evaluate the survival of patients with CRC, as determined by the Cancer Genome Atlas Program (TCGA) portal and gene expression profiling interactive analysis (GEPIA) databases. Western blotting and reverse transcription-polymerase chain reaction were performed to detect protein and mRNA levels of PPFIA1, PPFIA3, and PPFIA4, respectively. Immunohistochemical (IHC) staining was used to detect the correlation between PPFIA4 expression and the degree of CRC malignancy. Furthermore, potential miRNAs targeting PPFIA4 in CRCs were studied and confirmed.
Results
Bioinformatic analysis showed that the mRNA levels of PPFIA1, PPFIA3, and PPFIA4 were higher in CRC tissue samples than in normal colon tissue. Both mRNA and protein expression of PPFIA1, PPFIA3, and PPFIA4 were increased in the CRC cell lines LoVo and Hct116 compared with the normal colon epithelial cell line. Only PPFIA4 was associated with the prognosis of patients with CRC, which was confirmed by TCGA portal and GEPIA. IHC staining confirmed that the expression of PPFIA4 was higher in CRC tissues than in normal colon tissues and also increased in poorly differentiated CRC tissues and lymph node metastatic foci in comparison with well-differentiated CRC tissues and moderately differentiated CRC tissues. Functional annotation enrichment analysis indicated that the top 100 genes co-expressed with PPFIA4 were enriched in the G-protein coupled peptide receptor activity, leukotrience B4 receptor activity, and peroxisome proliferator-activated receptors and hypoxia-inducible factor-1 signaling pathways. In addition, miR-485-5p negatively regulates the expression of PPFIA4.
Conclusion
PPFIA4 expression is associated with the development of CRCs and may be a novel potential prognostic marker for human CRCs.
Research on scientific evaluation method of thermal characteristics of CNC machine tools based on robustness
Authors: Ran Jing , Miao Enming , Luo Hui , Tang Guangyuan 
Published: June 2022

Abstract
For the thermal test of CNC machine tools, the existing scientific researchers measured their main axis thermal deformation characteristics as a test index based on international standard ISO 230-3: 2020. However, according to the team's research, it is found that the thermal characteristics of machine tools reflected by the positions of different fixed points of different types of machine tools are quite different, and cannot fully and accurately reflect the thermal characteristics of machine tools. The thermal characteristics of modern machine tools are not only closely related to the structure, but also and the compensation model are inseparable. Therefore, on the basis of international standards, this paper selects three-axis and five-axis vertical machining centers as the research objects, And based on the current international advanced machining equipment robustness theory, combining the robustness model prediction effect and machine tool thermal characteristics, a scientific machine tool thermal characteristics detection method and evaluation standard are proposed, which can effectively demonstrate the actual performance of the machine tool. The research results show that the rigid and stable spindle of the three-axis CNC machining center can choose the fixed position of the worktable to evaluate the thermal characteristics of the machine tool, but it is necessary to optimize the position of the fixed point of the worktable according to the thermal characteristics prediction model. However, the rigidity of the spindle of the five-axis machining center is poor, which requires the modeling of the whole working area and the evaluation of thermal characteristics to be more scientific. Simply using the three-axis CNC machining center to select the fixed point of the worktable as the evaluation of the thermal characteristics of the machine tool has a large error.
Historical Landscape Evolution and Pattern Characteristics of Water System in Yuanmingyuan Garden
Authors: Zhang Shuai , Xi Xuesong , Huang Shiwei , Liang Shifan 
Published: June 2022

Abstract
Yuanmingyuan Garden embodies the outstanding wisdom and cultural and artistic pursuit of Chinese classical royal gardens in water management. The water system has rich morphological changes, vivid waterscape and profound water culture. The overall water system design realises the perfect combination of water function, culture, ecology and landscape. The current water system area accounts for about 30% of the total area of the park, which is a pattern formed after continuous construction, improvement, change and governance in various historical periods. This study adopts the method of combining historical documents, map and modern spatial information system to restore and overlay the water system pattern in the typical historical periods of Yuanmingyuan, such as the initial period, the peak period, the ruin period and the contemporary development period in the same spatial coordinate system. The pattern characteristics and evolution laws of the water system in each historical period were quantitatively analysed and explained by landscape pattern indexes, such as the type area of water patches and the proportion of water patches in water patch number. The causes and cultural connotation of water system pattern characteristics in various historical periods are interpreted in combination with the historical background and design concept.
Identification of Potential Inhibitors for Beta-Lactamase in Methicillin-
Resistant Staphylococcus aureus from Flavonoids Using a Computational
Drug Discovery Approach
Authors: Abdollahian Amirreza , Hazhirkamal Maryam , Taheri Mohammad , Nouri Fatemeh , Taherkhani Amir 
Published: June 2022

Background:
Staphylococcus aureus (S. aureus) is a Gram-positive bacterium causing a wide
range of human infections, leading to life-threatening invasive disorders, hospitalization, and mortality.
Producing β-lactamase enzymes in S. aureus is one of the main mechanisms of the pathogen that makes
the bacteria resistant to beta-lactam antibiotics, resulting in methicillin-resistant S. aureus (MRSA)
strains. Therefore, it is crucial to identify novel β-lactamase inhibitors to combat infections caused by
MRSA strains.

Methods:
In silico virtual screening approach was executed to evaluate the binding affinity of several
natural flavonoids to the MRSA β-lactamase active site. After that, the stability of interactions between
top inhibitors and the residues incorporated inside the β-lactamase was examined by molecular dynamics
(MD) simulation. Moreover, the most connected amino acid within the catalytic domain of the enzyme
was determined.

Results:
Rutin, isoquercitrin, nicotiflorin, quercetin-3-rhamnoside, vicenin-2, quercitrin, and orientin
demonstrated a salient binding affinity with the β-lactamase active site (ΔG binding < −10 kcal/mol). Interestingly,
the inhibition constant value (Ki) for rutin was estimated at the picomolar scale. The docked
poses of these compounds were demonstrated to be stable. Moreover, Gln237 was revealed to be the most
crucial residue involved in ligand binding.

Conclusion:
Rutin, isoquercitrin, nicotiflorin, quercetin-3-rhamnoside, vicenin-2, quercitrin, and orientin
may be potent inhibitors of β-lactamase and may be helpful for the treatment of several invasive infections
caused by MRSA strains. However, experimental studies are needed in the future to validate our
findings.
Machine-learning Algorithms for Ischemic Heart Disease Prediction:
A Systematic Review
Authors: Bani Hani Salam H. , Ahmad Muayyad M. 
Published: June 2022

Purpose:
This review aims to summarize and evaluate the most accurate machine-learning algorithm used to predict ischemic heart disease.

Methods:
This systematic review was performed following PRISMA guidelines. A comprehensive search was carried out using multiple databases such as Science Direct, PubMed\ MEDLINE, CINAHL, and IEEE explore.

Results:
Thirteen articles published between 2017 to 2021 were eligible for inclusion. Three themes were extracted: the commonly used algorithm to predict ischemic heart disease, the accuracy of algorithms to predict ischemic heart disease, and the clinical outcomes to improve the quality of care. All methods have utilized supervised and unsupervised machine-learning.

Conclusion:
Applying machine-learning is expected to assist clinicians in interpreting patients’ data and implementing optimal algorithms for their datasets. Furthermore, machine-learning can build evidence-based that support health care providers to manage individual situations who need invasive procedures such as catheterizations.
Diagnostic Role and Methods of Detection of Cardiac Troponins: An Opinion
from Historical and Current Points of View
Authors: Michailovich Chaulin Aleksey 
Published: June 2022

Abstract:
The laboratory methods for the determination of cardiac troponins (cTnI, cTnT) used nowadays are extremely diverse, which has a significant impact on our understanding of the biology and di¬agnostic the value of cTnI and cTnT as biomarkers. The main classifica¬tion of methods for the determination of cTnI and cTnT is based on the sensitivity of the immunoassay. Low- and moderately sensitive detec¬tion methods are known to be relatively low sensitive, which leads to a relatively late confirmation of cardiomyocyte death. Due to new highly sensitive methods used for the determination of cTnI and cTnT, designated as highly or ultrasensitive immunoassays (hs-TnT and hs-TnT), we received new, revised data about the biology of cardiac tro¬ponin molecules. In particular, it became clear that they can be considered products of normal myocardium metabolism since hs-TnT and hs-TnT are detected in almost all healthy patients. It also turned out that hs-TnT and hs-TnT are differ by gender (in men, troponin con¬centration in the blood is higher than in women), age (in elderly pa¬tients, the levels of troponins are higher than in young ones) and circadian cycles (morning concentrations of troponins are higher than in the evening). A large variety of methods for determining cTnI and cTnT, differing in their diagnostic capabilities, creates the need for tests to perform an unbiased assessment of the analytical charac¬teristics of each method. This review focuses on the most pressing is¬sues related to the discussion of the biological characteristics of cardiac troponin and the analytical characteristics of troponin immunoassays from a historical and contemporary point of view.
Development of a Fast and Highly Sensitive UPLC–MS/MS Technique for
Simultaneous Estimation of Artemether and Dihydroartemisinin with its
Application to Pharmacokinetic and Biodistribution Studies
Authors: Rahman Mohammad Akhlaquer 
Published: June 2022

Background:
Artemether (ART) has been recognized as a potent and rapidly acting
antimalarial agent metabolized by cytochrome P450 isoenzyme 3A4 into a more active form dihydroartemisinin
(DHA). Hence, it was required to study the pharmacokinetics and biodistribution
of ART and DHA for the optimization of dose.

Objectives:
The current research aims to develop an ultra-performance liquid chromatographytandem
mass spectrometry (UPLC-MS/MS) technique with high sensitivity for quantification of
ART and DHA simultaneous in the biological fluid.

Methods:
An isocratic elution mode was applied for delivery of mobile phase (A) ammonium acetate
(2mM) in UPLC grade water and (B) acetonitrile (20:80, v/v) comprising 0.1% v/v formic
acid in each solvent system with the rate of flow maintained at 400 μL/min. For analyte detection
and parent to daughter ion transition tracking, multiple reaction monitoring system was adopted.
Artemisinin was selected as the internal standard (IS) for its structural similarity with the analytes.

Results:
The linearity range of the calibration curve was between 0.5-200 ng/mL. The regression
equation indicated r2 = 0.996 for ART and r2 = 0.997 for DHA. Precision and accuracy set in
terms of relative error (RE%: ±15) and relative standard deviation (RSD%: ±15) expressed in
percentage were within the acceptability range. The % recoveries were within the acceptable limit
(90–110%). The detection and quantification limits of the analyte were 0.2 ng/mL and 0.5 ng/mL,
respectively.

Conclusion:
The method demonstrated a useful technique for quantification of ART and DHA
for evaluating the pharmacokinetics and biodistribution studies in rat plasma following orally
administered nanoformulation.
Novel Potent EGFR-JAK3 Dual-Target Inhibitor that Overcomes KRAS Mutation
Resistance in Colorectal Cancer
Authors: Chen Lixue , Ma Xiaodong , Sun Xiuli , Wu Tingyu , Yu Jiawen , Wang Changyuan , Jin Yue , Zheng Xu 
Published: June 2022

Background:
In-depth and clear mechanistic study is a prerequisite for new drugs to enter clinical research.

Method:
New chemical entity BY4008 was identified by our lab as a novel and highly potent EGFR and JAK3 dualtarget
inhibitor. A cell-based test exhibited strong antiproliferative activities against SW620 and HCT116 colon cancer
cells harboring KRAS mutation with IC50 of nanomolar potency. Furthermore, acridine orange/ethidium bromide
(AO/EB), Hematoxylin-Eosin (H&E) and DAPI staining assays and flow cytometry analyses indicated that BY4008
has the function of pro-apoptosis and arresting the cell cycle. In addition, BY4008 inhibited the autophosphorylation of
EGFR and blocked the activation of downstream signaling and the JAK-STAT3 pathway.

Result:
Meanwhile, a decreased level of reactive oxygen species (ROS) and an increased level of malondialdehyde
(MDA) in SW620 and HCT116 cells were observed after exposure to BY4008.

Conclusion:
In summary, this study provides an important structural basis and mechanistic study for future effective
treatment of colorectal cancer.