bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative dual-energy CT as a nondestructive tool to identify indicators for fossilized bone in vertebrate paleontology
Authors: Hamm Charlie A. , Hampe Oliver , Mews Jürgen , Günter Christina , Milke Ralf , Witzmann Florian , Savic Lynn J. , Hecht Lutz , Meister Sabine , Hamm Bernd , Asbach Patrick , Diekhoff Torsten 
Published: September 2022

AbstractDual-energy computed tomography (DECT) is an imaging technique that combines nondestructive morphological cross-sectional imaging of objects and the quantification of their chemical composition. However, its potential to assist investigations in paleontology has not yet been explored. This study investigates quantitative DECT for the nondestructive density- and element-based material decomposition of fossilized bones. Specifically, DECT was developed and validated for imaging-based calcium and fluorine quantification in bones of five fossil vertebrates from different geological time periods and of one extant vertebrate. The analysis shows that DECT material maps can differentiate bone from surrounding sediment and reveals fluorine as an imaging marker for fossilized bone and a reliable indicator of the age of terrestrial fossils. Moreover, the jaw bone mass of Tyrannosaurus rex showed areas of particularly high fluorine concentrations on DECT, while conventional CT imaging features supported the diagnosis of chronic osteomyelitis. These findings highlight the relevance of radiological imaging techniques in the natural sciences by introducing quantitative DECT imaging as a nondestructive approach for material decomposition in fossilized objects, thereby potentially adding to the toolbox of paleontological studies.
Muscle function and functional performance after pulmonary rehabilitation in patients with chronic obstructive pulmonary disease: a prospective observational study
Authors: Pancera Simone , Bianchi Luca N. C. , Porta Roberto , Villafañe Jorge H. , Buraschi Riccardo , Lopomo Nicola F. 
Published: September 2022

AbstractThis study aimed to measure changes in different properties of skeletal muscles and evaluate their contribution and relationship to changes in functional performance after pulmonary rehabilitation (PR) in patients with chronic obstructive pulmonary disease (COPD). COPD outpatients attending 5 weeks of conventional PR were recruited. Functional performance [5-repetitions sit-to-stand (5STS), and 4-m gait speed (4mGS)], and muscle function (maximal isometric strength, power, force control, and relative concentric and eccentric activation during 5STS) were assessed after PR and 3 months of follow-up. Twenty patients (71 years; 52% of predicted FEV1) completed the study. 4mGS and relative concentric activation during 5STS decreased respectively by 7.7% and 26% between the beginning of PR and follow-up. Quadriceps strength, power, and force control improved by 10.4%, 27.3%, and 15.2%, respectively, from the beginning of PR to follow-up the relative eccentric activation during 5STS explained 31% of the variance in 4mGS changes. In conclusion, functional performance appeared to decline after conventional PR, whereas several properties of skeletal muscles were maintained at follow-up in COPD outpatients. Of note, eccentric contractions might play a role in the improvement of functional performance. Therefore, future studies with interventional design should include eccentric training in PR programs during clinical COPD practice.
Lipidic profiles of patients starting peritoneal dialysis suggest an increased cardiovascular risk beyond classical dyslipidemia biomarkers
Authors: Lluesa Julia Hernández , López-Romero Luis Carlos , Monzó José Jesús Broseta , Marugán Marta Roca , Boyano Iris Viejo , Rodríguez-Espinosa Diana , Gómez-Bori Aina , Orient Amparo Soldevila , Such Ramón Devesa , Perez Pilar Sánchez , Jaras Julio Hernández 
Published: September 2022

AbstractPatients on peritoneal dialysis (PD) have an increased risk of cardiovascular disease (CVD) and an atherogenic lipid profile generated by exposure to high glucose dialysis solutions. In the general population, the reduction of classic lipids biomarkers is associated with improved clinical outcomes; however, the same results have not been seen in PD population, a lack of data this study aims to fulfill. Single-center prospective observational study of a cohort of CKD patients who started renal replacement therapy with continuous ambulatory peritoneal dialysis. The differences in the lipid profile and analytical variables before and 6 months after the start of peritoneal dialysis were analyzed. Samples were analyzed on an Ultra-Performance Liquid Chromatography system. Thirty-nine patients were enrolled in this study. Their mean age was 57.9 ± 16.3 years. A total of 157 endogenous lipid species of 11 lipid subclasses were identified. There were significant increases in total free fatty acids (p < 0.05), diacylglycerides (p < 0.01), triacylglycerides, (p < 0.01), phosphatidylcholines (p < 0.01), phosphatidylethanolamines (p < 0.01), ceramides (p < 0.01), sphingomyelins (p < 0.01), and cholesterol esters (p < 0.01) from baseline to 6 months. However, there were no differences in the classical lipid markers, neither lysophosphatidylcholines, monoacylglycerides, and sphingosine levels. 6 months after the start of the technique, PD patients present changes in the lipidomic profile beyond the classic markers of dyslipidemia.
Effects of different doses of synthetic oxytocin on neonatal instinctive behaviors and breastfeeding
Authors: Zhou Yun , Liu Wenwen , Xu Yang , Zhang Xiaoyan , Miao Yiqun , Wang Aihua , Zhang Yuanyuan 
Published: September 2022

AbstractSynthetic oxytocin is the current domestic first-line agent of induced labor and labor augmentation, and its potential effects on neonatal neurobehavioral development is currently attracting increased attention. To explore the effect of different doses of synthetic oxytocin on neonatal instinctive breastfeeding behavior and breastfeeding by observing neonatal behaviors during skin-to-skin contact with mothers after delivery. Observations and comparisons of neonatal instinctive behaviors were conducted by using Widström's 9 Stages method. According to the total dosage of oxytocin administered during labor, participants were divided into a low dose group (≤ 2.5 U) of 39 pairs, a medium dose group (> 2.5 U) of 38 pairs, a high dose group (> 7.5 U) of 38 pairs and a control group (no synthetic oxytocin use) of 39 pairs. The occurrence time of newborns' instinctive movements and the duration of each behavior stage for the four groups were also analyzed. The number of exclusive breastfeeding sessions within 3 days after birth and the rate of exclusive breastfeeding at 3 months were collected and compared. There were significant differences among the four groups in the occurrence time of raising head or turning head (p = 0.004), eating hands (p = 0.011), moving body (p = 0.001), locating areola (p < 0.001), licking nipples (p = 0.002), containing nipple (p = 0.001), sucking (p < 0.001). There were significant differences among the four groups in the duration of activity (p = 0.004), clawing (p = 0.001), familiarization (p = 0.001), and sucking (p < 0.001). There was also a significant difference in the number of exclusive breastfeeding sessions of 24 h (p = 0.011), 48 h (p < 0.001), 72 h (p = 0.001) after birth among the four groups, but there was no statistical difference in the rate of exclusive breastfeeding at 3 months after birth. The intrapartum administration of synthetic oxytocin was associated with the expression of neonatal instinctive breastfeeding. With increases in drug dose, the effect of breast seeking activity and breast attachment was more significant, and the association of synthetic oxytocin on sucking and breastfeeding was dose-dependent.
Mutations conferring SO42− pumping ability on the cyanobacterial anion pump rhodopsin and the resultant unique features of the mutant
Authors: Doi Yuhei , Watanabe Jo , Nii Ryota , Tsukamoto Takashi , Demura Makoto , Sudo Yuki , Kikukawa Takashi 
Published: September 2022

AbstractMembrane transport proteins can be divided into two types: those that bind substrates in a resting state and those that do not. In this study, we demonstrate that these types can be converted by mutations through a study of two cyanobacterial anion-pumping rhodopsins, Mastigocladopsis repens halorhodopsin (MrHR) and Synechocystis halorhodopsin (SyHR). Anion pump rhodopsins, including MrHR and SyHR, initially bind substrate anions to the protein center and transport them upon illumination. MrHR transports only smaller halide ions, Cl- and Br-, but SyHR also transports SO42−, despite the close sequence similarity to MrHR. We sought a determinant that could confer SO42− pumping ability on MrHR and found that the removal of a negative charge at the anion entrance is a prerequisite for SO42− transport by MrHR. Consistently, the reverse mutation in SyHR significantly weakened SO42− pump activity. Notably, the MrHR and SyHR mutants did not show SO42− induced absorption spectral shifts or changes in the photoreactions, suggesting no bindings of SO42− in their initial states or the bindings to the sites far from the protein centers. In other words, unlike wild-type SyHR, these mutants take up SO42− into their centers after illumination and release it before the ends of the photoreactions.
Comparison of image registration methods for combining laparoscopic video and spectral image data
Authors: Köhler Hannes , Pfahl Annekatrin , Moulla Yusef , Thomaßen Madeleine T. , Maktabi Marianne , Gockel Ines , Neumuth Thomas , Melzer Andreas , Chalopin Claire 
Published: September 2022

AbstractLaparoscopic procedures can be assisted by intraoperative modalities, such as quantitative perfusion imaging based on fluorescence or hyperspectral data. If these modalities are not available at video frame rate, fast image registration is needed for the visualization in augmented reality. Three feature-based algorithms and one pre-trained deep homography neural network (DH-NN) were tested for single and multi-homography estimation. Fine-tuning was used to bridge the domain gap of the DH-NN for non-rigid registration of laparoscopic images. The methods were validated on two datasets: an open-source record of 750 manually annotated laparoscopic images, presented in this work, and in-vivo data from a novel laparoscopic hyperspectral imaging system. All feature-based single homography methods outperformed the fine-tuned DH-NN in terms of reprojection error, Structural Similarity Index Measure, and processing time. The feature detector and descriptor ORB1000 enabled video-rate registration of laparoscopic images on standard hardware with submillimeter accuracy.
SNP discovery and association study for growth, fatness and meat quality traits in Iberian crossbred pigs
Authors: Óvilo C. , Trakooljul N. , Núñez Y. , Hadlich F. , Murani E. , Ayuso M. , García-Contreras C. , Vázquez-Gómez M. , Rey A. I. , Garcia F. , García-Casco J. M. , López-Bote C. , Isabel B. , González-Bulnes A. , Wimmers K. , Muñoz M. 
Published: September 2022

AbstractIberian pigs and its crosses are produced to obtain high-quality meat products. The objective of this work was to evaluate a wide panel of DNA markers, selected by biological and functional criteria, for association with traits related to muscle growth, fatness, meat quality and metabolism. We used 18 crossbred Iberian pigs with divergent postnatal growth patterns for whole genome sequencing and SNP discovery, with over 13 million variants being detected. We selected 1023 missense SNPs located on annotated genes and showing different allele frequencies between pigs with makerdly different growth patterns. We complemented this panel with 192 candidate SNPs obtained from literature mining and from muscle RNAseq data. The selected markers were genotyped in 480 Iberian × Duroc pigs from a commercial population, in which phenotypes were obtained, and an association study was performed for the 1005 successfully genotyped SNPs showing segregation. The results confirmed the effects of several known SNPs in candidate genes (such as LEPR, ACACA, FTO, LIPE or SCD on fatness, growth and fatty acid composition) and also disclosed interesting effects of new SNPs in less known genes such as LRIG3, DENND1B, SOWAHB, EPHX1 or NFE2L2 affecting body weight, average daily gain and adiposity at different ages, or KRT10, NLE1, KCNH2 or AHNAK affecting fatness and FA composition. The results provide a valuable basis for future implementation of marker-assisted selection strategies in swine and contribute to a better understanding of the genetic architecture of relevant traits.
Optical heating and luminescence thermometry combined in a Cr3+-doped YAl3(BO3)4
Authors: Elzbieciak-Piecka K. , Marciniak L. 
Published: September 2022

AbstractThe possibility of optical heating with simultaneous control of the generated light within a single phosphor is particularly attractive from the perspective of multiple applications. This motivates the search for new solutions to enable efficient optical heating. In response to these requirements, based on the high absorption cross-section of Cr3+ ions, the optical heater based on YAl3(BO3)4:Cr3+ exhibiting highly efficient heating is developed. At the same time, the emission intensity ratio of 2E(g) → 4A2(g) and 4T2(g) → 4A2(g) of Cr3+ bands, thanks to the monotonic temperature dependence, enables remote temperature readout of the phosphor using luminescence thermometry technique. The combination of these two functionalities within a single phosphor makes YAl3(BO3)4:Cr3+ a promising, self thermally controlled photothermal agent.
Evaluation of electrolyte element composition in human tissue by laser-induced breakdown spectroscopy (LIBS)
Authors: Winnand Philipp , Boernsen K. Olaf , Bodurov Georgi , Lammert Matthias , Hölzle Frank , Modabber Ali 
Published: September 2022

AbstractLaser-induced breakdown spectroscopy (LIBS) enables the direct measurement of cell electrolyte concentrations. The utility of LIBS spectra in biomarker studies is limited because these studies rarely consider basic physical principles. The aim of this study was to test the suitability of LIBS spectra as an analytical method for biomarker assays and to evaluate the composition of electrolyte elements in human biomaterial. LIBS as an analytical method was evaluated by establishing KCl calibration curves to demonstrate linearity, by the correct identification of emission lines with corresponding reference spectra, and by the feasibility to use LIBS in human biomaterial, analyzing striated muscle tissues from the oral regions of two patients. Lorentzian peak fit and peak area calculations resulted in better linearity and reduced shot-to-shot variance. Correct quantitative measurement allowed for differentiation of human biomaterial between patients, and determination of the concentration ratios of main electrolytes within human tissue. The clinical significance of LIBS spectra should be evaluated using peak area rather than peak intensity. LIBS might be a promising tool for analyzing a small group of living cells. Due to linearity, specificity and robustness of the proposed analytical method, LIBS could be a component of future biomarker studies.
Isolation and characterization of a Vibrio owensii phage phi50-12
Authors: Lin Ling-Chun , Tsai Yu-Chuan 
Published: September 2022

AbstractVibrio owensii is a widely distributed marine vibrio species that causes acute hepatopancreatic necrosis in the larvae of Panulirus ornatus and Penaeus vannamei, and is also associated with Montipora white syndrome in corals. We characterized V. owensii GRA50-12 as a potent pathogen using phenotypic, biochemical, and zebrafish models. A virulent phage, vB_VowP_phi50-12 (phi50-12), belonging to the N4-like Podoviridae, was isolated from the same habitat as that of V. owensii GRA50-12 and characterized. This phage possesses a unique sequence with no similar hits in the public databases and has a short latent time (30 min), a large burst size (106 PFU/infected cell), and a wide range of pH and temperature stabilities. Moreover, phi50-12 also demonstrated a strong lysis ability against V. owensii GRA50-12. SDS-PAGE revealed at least nine structural proteins, four of which were confirmed using LC–MS/MS analysis. The size of the phi50-12 genome was 68,059 bp, with 38.5% G + C content. A total of 101 ORFs were annotated, with 17 ORFs having closely related counterparts in the N4-like vibrio phage. Genomic sequencing confirmed the absence of antibiotic resistance genes or virulence factors. Comparative studies have shown that phi50-12 has a unique genomic arrangement, except for the well-conserved core regions of the N4-like phages. Phylogenetic analysis demonstrated that it belonged to a group of smaller genomes of N4-like vibrio phages. The therapeutic effect in the zebrafish model suggests that phi50-12 could be a potential candidate for application in the treatment of V. owensii infection or as a biocontrol agent. However, further research must be carried out to confirm the efficacy of phage50-12.
Cross-sectional study of passive opiate smoking in relation to stroke and some of stroke attributable risk factors in women
Authors: Jalali Nazanin , Khalili Parvin , Bahrampour Saeed , Mahmoudabadi Mohammad , Esmaeili Nadimi Ali , Jalali Zahra 
Published: September 2022

AbstractOpiate use is related to neuropathological disorders, stroke and stroke attributable risk factors. However, secondary exposure to opiate in relation to the above-mentioned complications is studied only in animal models and remains to be evaluated in human populations. We tested whether passive exposure to opiate is associated with stroke and the known stroke predictive factors. We carried out a cross-sectional study of 1541 never smoker women who participated in the Rafsanjan Cohort Study (RCS) with their husbands (2015–2017 recruitment phase). RCS is one of the 19 geographic districts of the Prospective Epidemiological Research Studies in Iran (PERSIAN cohort study). Unadjusted and adjusted multiple logistic regression analyses were performed to evaluate the relationship between second-hand opiate exposure (husband opiate smoking after marriage) and the odds ratio of stroke and the following stroke risk factors and predictive parameters: overweight/obesity (BMI > 25), cholesterol (chol) > 200 mg/dl, fasting blood sugar (FBS) > 125 mg/dl, low density lipoprotein (LDL) > 100 mg/dl, triglyceride (TG) >  = 150 mg/dl, hypertension, diabetes, and chronic headache. We observed a significant increased adjusted odds ratio (OR) of stroke (OR = 3.43, 95% CI:1.33–8.82) and its risk factors LDL > 100 mg/dl (OR = 1.37, 95% CI:1.01–1.87) and FBS > 125 mg/dl (OR = 1.58, 95% CI:1.08–2.30) in women associated with husbands’ opiate smoking. This relationship was observed after adjusting for the confounding parameters including age, education years, and first-degree family history of the relevant diseases. The increased odds ratio for stroke and high LDL displayed a dose-sensitive trend with years of husband’s opiate smoking after marriage (respective p-trends: 0.02 & 0.01). We did not observe a significant association between passive opiate smoking and high TG, high Chol or the diseases diabetes, hypertension and chronic headache. However, 89% increased odds ratio of chronic headache was observed to be associated with passive opiate smoking for more than 10 years (OR = 1.89, 95% CI:1.02–3.50). We found an increased risk of stroke and high LDL and FBS in women associated with passive opiate smoking. Furthermore, a dose-sensitive connection was found between the risks of stroke, high LDL and chronic headache with the years of passive opiate exposure. Our results point to the necessity of the future analyses, which further assess whether passive opiate exposure could be considered as an independent risk factor for stroke and metabolic diseases.
Spatial distribution characteristics and evaluation of soil pollution in coal mine areas in Loess Plateau of northern Shaanxi
Authors: Wang Na , Luo Yuhu , Liu Zhe , Sun Yingying 
Published: September 2022

AbstractThe ecological environment in Loess Plateau of Northern Shaanxi is fragile, so the soil pollution caused by the exploitation of coal resources cannot be ignored. With Shigetai Coal Mine in Loess Plateau of Northern Shaanxi as the object of study for field survey and sampling, the content of heavy metals in soil is analyzed, the environmental pollution in the research area is evaluated by the single factor pollution index method, comprehensive pollution index method and potential ecological risk index method, and the spatial distribution characteristics of heavy metals are discussed by the geostatistics method. According to the study results, the average contents of heavy metals Hg, Cd, Pb and Cr are 2.03, 1.36, 1.11 and 1.23 times of the soil background values in Shaanxi Province respectively and the average contents of other heavy metals are lower than the soil background values in Shaanxi Province; Hg and Cd show moderate variation while As, Pb, Cr, Zn, Ni and Cu show strong variation; the skewness coefficients and kurtosis coefficient of Cd, As and Cu in the soil within the research area are relatively high, and these elements are accumulated in large amounts. Single factor pollution index (Pi) and potential ecological risk index (E) indicate that heavy metal Hg is the main pollution factor and mainly distributed in the east and north of the research area. The comprehensive index of potential ecological risk (RI) of the research area is 1336.49, showing an extremely high ecological risk, and the distribution characteristics of potential ecological risk are consistent with that of potential ecological risk index (E) of Hg. The results of ecological risk warning show that Hg is in a slight warning status, while Cd, Pb and Cr are in a warning status. The areas with high ecological risk warning values are mainly distributed in the east and north, and the whole research area shows relatively obvious zonal distribution law. The soil is disturbed greatly during the coal mining, so the ecological governance of the mine area shall adapt to the local natural conditions and regional environmental characteristics and follow the principle of “adjusting governance measures based on specific local conditions and classifications”. An environmentally sustainable governance manner shall be adopted to realize the protection of the ecological environment and high-quality development of coal resources.