bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhyzopertha dominica (F., 1792) (Coleoptera: Bostrichidae)’nın Türkiye popülasyonlarındaki fosfin direncinde bioassay ile moleküler markörün karşılaştırılması
Authors: YILMAZ Abdullah , KOÇAK Erhan 
Published: November 2022

Fosfin gazı depolanmış hububattaki böceklere karşı dünya genelinde kullanılan ana pestisittir. Türkiye’de de 1950’lerden itibaren kullanılmaktadır. Bu fumiganta karşı dünya genelinde böceklerde direnç artışı önemli bir problemdir. Bu çalışmada ekin kambur böceği, Rhyzoperta dominica (F., 1792) (Coleoptera: Bostrichidae)’nın ülkemizde 12 ilden 18 popülasyonundaki fosfin dirençleri 2013-2017 yılları arasında belirlenmiştir. Ayırıcı doz çalışmaları 15 popülasyonda fosfin direnci geliştiğini göstermiştir. Bu popülasyonlarda doz-yanıt bioassayleri, direnç oranlarının 96-533 kat arasında değiştiğini göstermiştir. Ayrıca, fosfin direncine sahip bu popülasyonlarda DLD (dihydrolipoamide dehydrogenase) geninde amino asit mutasyonunu gösteren mevcut moleküler direnç markörü P49S test edilmiştir. R direnç alleli bu 15 popülasyonda yüksek frekansta (%83.7) belirlenmişken hassas olan üç popülasyonda ise belirlenmemiştir. Dirençli popülasyonlardaki 324 bireyden elde edilen genetic sonuçlara göre homozigot direnç, heterozigot direnç ve homozigot hassas allel oranları sırasıyla %62.0, 18.9 ve 19.1 olarak belirlenmiştir. Türkiye R. dominica popülasyonlarında genetik markör ile fosfin direncini belirleme sonuçlarının bioassay sonuçlarıyla kıyaslanabilir olduğu görülmüştür. Sonuçta, fosfin direncinin genetik olarak testlenmesi Türkiye’de direnç yönetimini kolaylaştıracaktır.
THE ROLE OF CHINA AND RUSSIA IN THE ASIAN-PACIFIC REGION AND TRENDS IN CHINESE-RUSSIAN RELATIONS IN THE CONTEXT OF THE UKRAINIAN CRISIS
Authors: Zhao Lu , Wu Yanbin 
Published: November 2022

Aim. To study the role of China and Russia in the Asia-Pacific region and trends in the development of Sino-Russian relations in the context of the Ukrainian crisis. Research objective is to explain the current political interaction between China and Russia, to analyze the strategic goals of China, Russia and the United States in the Asia-Pacific region, and also to interpret the perception of China and Russia of the global political situation in the context of the Ukrainian crisis, especially in the Asia-Pacific region, in order to generalize the role of China and Russia in this region and the trends of Sino-Russian relations in the context of the Russian-Ukrainian conflict.Methodology. Methods of comparative analysis of data contained in various sources, such as the literature method of research and content analysis, were applied. The methodological basis of the work is the empirical method of research.Results. Based on the analysis of the literature and the media, it was concluded that Russia plays an indispensable role in maintaining security and strategic stability in the Asia-Pacific region, and China has a decisive influence on the political, economic and security spheres of the Asia-Pacific region. At the same time, in the context of the Ukrainian crisis, Sino-Russian relations will continue to be based on national strategic interests and will hardly change due to the Russian-Ukrainian conflict and the EU and USA provocations.Research implications. The materials of the article are new and correspond to current political hotspots, they have an auxiliary reference effect for relevant researchers.
Autologous Costal Cartilage Grafting for a Large Osteochondral Lesion of the Femoral Head
Authors: Zhang Chang-Qing , Du Da-Jiang , Hsu Pei-Chun , Song Yan-Yan , Gao Yun , Zhu Zhen-Zhong , Jia Wei-Tao , Gao You-Shui , Zheng Ming-Hao , Zhu Hong-Yi , Hsiang Fu-Chou , Chen Sheng-Bao , Jin Dong-Xu , Sheng Jia-Gen , Huang Yi-Gang , Feng Yong , Gao Jun-Jie , Li Guang-Yi , Yin Ji-Min , Yao Chen , Jiang Chen-Yi , Luo Peng-Bo , Tao Shi-Cong , Chen Chun , Zhu Jin-Yu , Yu Wei-Bin 
Published: November 2022

Background:
There is currently no ideal treatment for osteochondral lesions of the femoral head (OLFH) in young patients.

Methods:
We performed a 1-year single-arm study and 2 additional years of follow-up of patients with a large (defined as >3 cm2) OLFH treated with insertion of autologous costal cartilage graft (ACCG) to restore femoral head congruity after lesion debridement. Twenty patients ≤40 years old who had substantial hip pain and/or dysfunction after nonoperative treatment were enrolled at a single center. The primary outcome was the change in Harris hip score (HHS) from baseline to 12 months postoperatively. Secondary outcomes included the EuroQol visual analogue scale (EQ VAS), hip joint space width, subchondral integrity on computed tomography scanning, repair tissue status evaluated with the Magnetic Resonance Observation of Cartilage Repair Tissue (MOCART) score, and evaluation of cartilage biochemistry by delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) and T2 mapping.

Results:
All 20 enrolled patients (31.02 ± 7.19 years old, 8 female and 12 male) completed the initial study and the 2 years of additional follow-up. The HHS improved from 61.89 ± 6.47 at baseline to 89.23 ± 2.62 at 12 months and 94.79 ± 2.72 at 36 months. The EQ VAS increased by 17.00 ± 8.77 at 12 months and by 21.70 ± 7.99 at 36 months (p < 0.001 for both). Complete integration of the ACCG with the bone was observed by 12 months in all 20 patients. The median MOCART score was 85 (interquartile range [IQR], 75 to 95) at 12 months and 75 (IQR, 65 to 85) at the last follow-up (range, 24 to 38 months). The ACCG demonstrated magnetic resonance properties very similar to hyaline cartilage; the median ratio between the relaxation times of the ACCG and recipient cartilage was 0.95 (IQR, 0.90 to 0.99) at 12 months and 0.97 (IQR, 0.92 to 1.00) at the last follow-up.

Conclusions:
ACCG is a feasible method for improving hip function and quality of life for at least 3 years in young patients who were unsatisfied with nonoperative treatment of an OLFH. Promising long-term outcomes may be possible because of the good integration between the recipient femoral head and the implanted ACCG.

Level of Evidence:
Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Incremental constraint projection algorithm for strongly pseudomonotone variational inequalities *
Authors: Ma Beibei , Wang Wanyu 
Published: November 2022

Abstract
In this paper, we propose a new incremental constraint projection method for strongly pseudomonotone variational inequalities with special structure , which the constraint set X is the intersection of a large number of closed convex sets. Our method only computes two halfspace projections rather than the projections of the full or single constraint set at each iteration. We prove that the sequence generated by our method converges almost surely to a solution of the variational inequalities without the strongly monotonicity or monotone plus assumption of the mapping. We also analyze the convergence rate of the iterative sequence. Numerical experiments are reported to illustrate the effectiveness of the proposed method.
Achieving CD8+ T cell-dependent lethality by targeting cancer USP14 in hepatocellular carcinoma
Authors: Li Binkui , Yuan Yichuan , Niu Yi , Huang Zhenkun , Shi Yunxing , Lin Zhu , Zuo Dinglan , Zhong Chengrui , Li Kai , Yuan Yun-Fei , Guan Xin-Yuan 
Published: November 2022

Abstract
Background & aims: The application of immunotherapy in hepatocellular carcinoma has been hindered by resistance to therapeutics. Cancer cell-induced CD8+ T cell malfunction may account for the failure of tumor immunotherapies. The crucial regulators in HCC that mediate resistance to CD8+ T cell-based cancer immunotherapy are still obscure.
Methods: Three independent CRISPR genome-wide screening was constructed. HCC cell-CD8+T cell co-culture model and mouse HCC models were performed to explore the effect of cancer USP14 in vitro and in vivo. Metabolomics, glucose up-take analysis and immune co-precipitation were performed for mechanismstudy. scRNA-seq, HCC PDO and PDX models were used to validate the therapeutic potential of targeting cancer USP14.
Results: USP14 was identified as an essential driver of HCC cells tolerating to CD8+ T cells. Cancer USP14 was upregulated and positively correlated with resistance to anti-PD1 therapy and poor prognoses in HCC patients. Inhibition of cancer USP14 reduced the growth of HCC cell with CD8+ T cell co-culturing and enhanced the function of co-cultured CD8+ T cells. Targeting cancer USP14 inhibited HCC tumor growth and boosted anti-PD1-induced tumor immunity in vivo. USP14 highly-expressed HCC cells were avidly consumed and outcompeted CD8+ T cells for glucose by stabilizing GLUT1, resulting in CD8+ T cells starving and malfunction. These HCC cells also lead to acidification in the TME, causing CD8+ T cell dysfunction. The PDO and PDX models confirmed the above results.
Conclusions:Our data defined a pro-immunoevasiverole for cancer USP14 in CD8+ T cell-based tumor immunity. Combining USP14 inhibition and PD1 blockade constitutes a potential therapy for HCC.
Lay summary: Cancer USP14 sever as a potential therapeutic target for HCC.
What do we know about research on carbon capture, utilization and storage (CCUS) in China and the United States? Insights from the systematic review
Authors: ren qiang , Wei Shan sen , Du Jian hui , wu peng 
Published: November 2022

Abstract
Carbon dioxide capture, utilization and storage (CCUS) technology is an emerging technology with large-scale emission reduction potential and an essential component of the global response to climate change to achieve carbon neutrality goals. As the two most important countries in global climate governance, it is an exciting task to systematically review and examine the current status and trends of research in the field of CCUS in China and the United States. This paper used bibliometric tools to systematically sort out and analyze the peer-reviewed articles on the Web of Science in China and the United States during 2000–2022. It provides the first quantitative analysis of the current status and recent trends of CCUS technology in China and the United States regarding research institutions, major groups and core authors, highly cited papers, influential journals, disciplinary distribution, research hotspots, and topic clustering. The results show a significant increase in research interest in this topic among scholars from both countries. The number of publications in the CCUS field in China and the United States was 1196 and 1302, respectively, showing an increasing trend. China and the United States have become the most influential countries in the area of CCUS. And the United States has a more significant academic influence on a global scale. Based on the differences in the national conditions of the two countries, the research hotspots in the field of CCUS in China and the United States show the characteristics of diversification and differentiation. China and the United States pay attention to different research hotspots or have different focuses in different periods. This paper also finds that new capture materials and technology development, geological storage monitoring and early warning, CO2 utilization and new energy development, sustainable business models, incentive policies and measures, and public awareness are critical directions for future research in the field of CCUS. To provide a comprehensive review and comparison of CCUS technology development in China and the United States. It helps to gain insight into the research differences and linkages between the two countries in the field of CCUS and identify the research gaps between them. And place some consensus that policymakers can use.
Pan-cancer analysis identifies SEC11A as an immunological and prognostic biomarker
Authors: Wu qingqing , Liu chengdong , Huang guangzhao , Lu xinyan , Zhang yu , Li na , Lv xiaozhi , Shao tingru 
Published: November 2022

Abstract
Objective: Limited evidence suggests a vital role for SEC11A in carcinogenesis. Our study conducted an integrated analysis on the molecular features and clinical relevance of SEC11A across pan-cancer based on multi-omics data.
Materials and Methods: SEC11A expression was analyzed in 33 types of tumor and normal specimens utilizing transcriptome profiles from TCGA-GTEx project and protromic data from CPTAC project. Copy number alterations and methylation of SEC11A were investigated across pan-cancer with cBioPortal. Clinical relevance and prognostic implications of SEC11A were evaluated in TCGA cohorts. GSEA of SEC11A was conducted in head and neck squamous cell carcinoma (HNSCC) with clusterProfiler package. Correlation of SEC11A with immune cell infiltrations was estimated with EPIC, TIMER, xCELL, MCP-counter, quanTIseq, CIBERSORT, and ImmuCellAI algorithms. IC50 values of anti-cancer agents were curated from GDSC project and their correlations to SEC11A were calculated.
Results: We found the expression of SEC11A was upregulated in most of cancer types (24/33), while was downregulated in 3 types of cancers. Genetic amplification in SEC11A was widespread across pan-cancer. There were negative correlations of SEC11A RNA expression with methylation level. The upregulation of SEC11A indicated unfavorable prognosis in adrenocortical carcinoma and HNSCC. Moreover, SEC11A was closely involved in cell cycle, TP53 regulation and antigen processing. SEC11A was positively associated with tumor-associated macrophage and fibroblast but negatively associated with CD8+ T cell. The expression of SEC11A was positively correlated to drug resistance to anticancer drugs.
Conclusion: These findings suggested that SEC11A could act as a prognostic biomarker across pan-cancer. Upregulation of SEC11A was in relation to tumor immunosuppressive status. Collectively, our results offered a valuable resource that might guide mechanistic and therapeutic analysis of SEC11A across cancers.
Identification and characterization of two salmon louse (Lepeophtheirus salmonis, Krøyer, 1838) heme peroxidases and their potential as vaccine antigens
Authors: Gislefoss Elisabeth , Gamil Amr Ahmed Abdelrahim , Øvergård Aina-Cathrine , Evensen Øystein 
Published: November 2022

Abstract
Salmon louse, Lepeophtheirus salmonis, represents a major challenge for salmon farming as current treatments impose welfare issues and are costly, and no prophylactic measures are available. Two salmon louse heme peroxidases (LsPxtl-1 and LsPxtl-2) were tested for their importance in parasite development in vitro and as potential vaccine candidates. LsPxtl-1 possesses two heme peroxidase domains and is expressed in ovaries and gut. LsPxtl-1 knockdown in nauplius II stage resulted in poor swimming performance and parasite death when reaching the copepodid stage, indicating LsPxtl-1 being indispensable for parasite development. LsPxtl-2 encodes one peroxidase domain and was predicted to contain an N-terminal signal peptide and an Eph receptor ligand binding domain. LsPxtl-2 knockdown did not impact survival or phenotype. Immunization with DNA plasmids encoding the peroxidases by single or combined injections, gave non-significant reduction in lice numbers post challenge which correlated with low specific antibody levels post vaccination. Combined injections of both plasmids followed by an LsPxtl-2 protein boost induced higher antibody response with reduced lice numbers post challenge but not significantly different from controls. The findings suggest LsPxtl-1 to play a role for parasite development, current formulations and vaccination modalities did not give significant reduction in lice infestation.
Shift current photovoltaic efficiency of 2D materials
Authors: Pedersen Thomas , Sauer Mikkel , Taghizadeh Alireza , Holguin Urko , Ovesen Martin , Thygesen Kristian , Olsen Thomas , Cornean Horia 
Published: November 2022

Abstract
Shift current photovoltaic devices are potential candidates for future cheap, sustainable, and
efficient electricity generation. In the present work, we calculate the solar-generated shift current
and efficiencies in 326 different 2D materials obtained from the computational database C2DB. We
apply, as metrics, the efficiencies of monolayer and multilayer samples. The monolayer efficiencies
are generally found to be low, while the multilayer efficiencies of infinite stacks show great promise.
Furthermore, the out-of-plane shift current response is considered, and material candidates for
efficient out-of-plane shift current devices are identified. Among the screened materials, MXY
Janus and MX2 transition metal dichalchogenides (TMDs) constitute a prominent subset, with
chromium based MXY Janus TMDs holding particular promise. Finally, in order to explain the
band gap dependence of the PV efficiency, a simple gapped graphene model with a variable band
gap is established and related to the calculated efficiencies.
HIV specific Th1 responses are altered in Ugandans with schistosoma mansoni coinfection
Authors: Obuku Andrew Ekii , Lugemwa Jacqueline Kyosimiire , Abaasa Andrew , Joloba Moses , Ding Song , Pollara Justin , Pantaleo Giuseppe , Ferrari Guido , Harari Alexandre , Kaleebu Pontiano 
Published: November 2022

Abstract
Fishing communities surrounding Lake Victoria in Uganda have HIV prevalence of 28% and incidence rates of 5 per 100 person years. More than 50% of the local fishermen are infected with Schistosoma mansoni (S. mansoni). We investigated the role of S. mansoni coinfection as a possible modifier of immune responses against HIV. Using polychromatic flow cytometry and Gran-ToxiLux assays, HIV specific responses, T cell phenotypes, antibody-dependent cell-mediated cytotoxic (ADCC) potency and titres were compared between participants with HIV-S. mansoni coinfection and participants with HIV infection alone. S. mansoni coinfection was associated with a modified pattern of anti-HIV responses, including lower frequency of bifunctional (IFNγ + IL-2 − TNF-α+) CD4 T cells, higher overall CD4 T cell activation and lower HIV ADCC antibody titres, compared to participants with HIV alone. These results support the hypothesis that S. mansoni infection affects T cell and antibody responses to HIV in coinfected individuals.
Constructing a Knowledge Graph from OpenStatistical Data: The Case of Nova ScotiaDisease Datasets
Authors: Rajabi Enayat , Midha Rishi , de Souza Jairo Francisco 
Published: November 2022

Abstract
The majority of available datasets in open government dataare statistical. They are widely published by various governments to beused by the public and data consumers. However, most open data por-tals do not provide the five-star Linked Data standard datasets. Thepublished datasets are isolated from one another while conceptually con-nected. Through this paper, a knowledge graph is constructed for thedisease-related datasets of a Canadian government data portal, NovaScotia Open Data. We leverage the Semantic Web technologies to trans-form the disease-related datasets into the Resource Description Frame-work (RDF) standard and enrich them with semantic rules. An RDFdata model using the RDF Cube vocabulary is designed in this work todevelop the graph that adheres to best practices and standards, allowingfor expansion, modification and flexible re-use 3. The study also discussesthe lessons learned during the cross-dimensional knowledge graph con-struction and integrating open statistical datasets from multiple sources.
Specific Codons Control Cellular Resources and Fitness
Authors: Love Aaron , Nair Nikhil 
Published: November 2022

Abstract
There is a degeneracy in codons – but they are not equivalent. While there is an understanding that codon use is unequal in native genes, there is less knowledge of how this usage bias modulates the supply and demand of protein translation resources. Here we investigate how the partitioning of microbial translational resources, specifically through allocation of tRNA by incorporating dissimilar codon usage bias, can drastically alter expression of proteins and reduce the burden on the host resources. By isolating individual codons experimentally, we find heterologous gene expression can trans-regulate fitness of the host and other heterologous genes. Interestingly, specific codons drive profitable or catastrophic phenotypic outcomes. We correlate codon usage patterns with genetic fitness and empirically derive a novel coding scheme for multi-gene expression called Codon Harmony Index (CHI, χ). CHI enables the design of harmonious multi-gene expression systems while avoiding catastrophic cellular burden.
TMJ degenerative joint disease: Relationships between CBCT findings, clinical symptoms, and signs
Authors: Yap Adrian Ujin , Lei Jie , Zhang Xiao-Han , Fu Kai-Yuan 
Published: November 2022

Abstract
Objectives: The relationships between CBCT findings, Temporomandibular disorder (TMD) symptoms, and signs were investigated in patients with TMJ degenerative joint disease (DJD).
Materials and Methods: Adult patients with DC/TMD-defined intra-articular conditions were enrolled and subjected to CBCT assessment. The participants were organized into three groups, namely no (NT), early (ET), and late (LT) TMJ DJD based on radiographic findings. TMD symptoms/signs were appraised using the DC/TMD Symptom Questionnaire and examination protocol respectively. Statistical analyses were performed using Chi-square/non-parametric tests and Kappa statistics (α = 0.05).
Results: The mean age of the participants (n = 877) was 30.60 ± 11.50 years (86.6% women). NT, ET, and LT were observed in 39.7%, 17.0%, and 43.3% of the study sample. Significant differences in the prevalence of TMD symptoms (TMD pain, TMJ sounds, opening, and closing difficulty) and signs (TMD/TMJ pain, TMJ clicking/crepitus, and opening limitation) were discerned among the three groups. TMD/TMJ pain and opening difficulty/limitation were more prevalent in early rather than late degenerative changes. While moderate agreements between symptoms and signs were observed for TMD pain/opening limitation, the concurrence for TMJ sounds was fair.
Conclusions: Patients with TMJ sounds and pain should be routinely examined with CBCT to establish the extent/progress of osseous changes.
Clinical Relevance:
Patients with TMJ sounds and TMD pain, particularly TMJ crepitus and pain, should be routinely examined with CBCT so that early and accurate TMJ DJD diagnoses can be made. This will facilitate timely therapeutic interventions to minimize further TMJ deterioration and encourage TMJ repair/regeneration.