bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear lamina strain states revealed by intermolecular force biosensor
Authors: Danielsson Brooke E. , Abraham Bobin George , Mäntylä Elina , Cabe Jolene I. , Mayer Carl R , Rekonen Anna , Ek Frans , Conway Daniel E. , Ihalainen Teemu O. 
Published: March 2022

AbstractNuclear lamins have been considered to be an important structural element of the nucleus. The nuclear lamina is thought both to shield DNA from excessive mechanical forces and to transmit mechanical forces onto the DNA. However, to date there is not yet a technical approach to directly measure mechanical forces on nuclear lamins at the protein level. To overcome this limitation, we developed a nanobody-based intermolecular tension FRET biosensor capable of measuring the mechanical strain of lamin filaments. Using this sensor, we were able to show that the nuclear lamina is subjected to significant force. These forces are dependent on nuclear volume, actomyosin contractility, functional LINC complex, chromatin condensation state, cell cycle, and EMT. Interestingly, large forces were also present on nucleoplasmic lamins, indicating that these lamins may also have an important mechanical role in the nucleus. Overall, we demonstrate that nanobody-based approach allows construction of novel force biosensors for mechanobiology studies.
The CIpP activator, TR-57, is highly effective as a single agent and in combination with venetoclax against CLL cells in vitro
Authors: Fatima Narjis , Shen Yandong , Crassini Kyle , Iwanowicz Edwin J. , Lang Henk , Karanewsky Donald S. , Christopherson Richard I , Mulligan Stephen P , Best O. Giles 
Published: March 2022

AbstractDespite advances in treatment, a significant proportion of patients with chronic lymphocytic leukaemia (CLL) will relapse with drug-resistant disease.Recent studies demonstrate that the imipridones ONC-201 and ONC-212 and the more potent TR-compounds are effective against a range of different cancers, including acute myeloid leukaemia and tumours of the brain, breast, and prostate. These drugs induce cell death through inhibition of mitochondrial function and activation of the mitochondrial protease, caseinolytic protease (CIpP), and the unfolded protein response (UPR).Here we demonstrate that a drug in this class, TR-57, has efficacy as a single agent and is synergistic with venetoclax against CLL cells cultured under in vitro conditions that mimic the tumour microenvironment. The inhibitory effects of TR-57 on cell survival, proliferation and migration were irrespective of poor-risk features, including aberrations of TP53. Changes in protein expression suggest the mechanisms of action of TR-57 and its synergy with venetoclax involve activation of the UPR, inhibition of the AKT and ERK1/2 pathways and a pro-apoptotic shift in expression of proteins of the BCL-2 family.The study suggests TR-57, as a single agent and in combination with venetoclax, may represent an effective treatment option for CLL, including for patients with poor-risk disease.
Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag
Authors: Bartosovic Marek , Castelo-Branco Gonçalo 
Published: March 2022

AbstractProbing epigenomic marks such as histone modifications at a single cell level in thousands of cells has been recently enabled by technologies such as scCUT&Tag. Here we developed a multimodal and optimized iteration of scCUT&Tag called nano-CT (for nano-CUT&Tag) that allows simultaneous probing of three epigenomic modalities at single-cell resolution, using nanobody-Tn5 fusion proteins. nano-CT is compatible with starting materials as low as 25 000 cells and has significantly higher resolution than scCUT&Tag, with a 16-fold increase in the number of fragments per cells. We used nano-CT to simultaneously profile chromatin accessibility, H3K27ac and H3K27me3 in a complex tissue - juvenile mouse brain. The obtained multimodal dataset allowed for discrimination of more cell types/states that scCUT&Tag, and inference of chromatin velocity between ATAC and H3K27ac in the oligodendrocyte (OL) lineage. In addition, we used nano-CT to deconvolute H3K27me3 repressive states and infer two sequential waves of H3K27me3 repression at distinct gene modules during OL lineage progression. Thus, given its high resolution, versatility, and multimodal features, nano-CT allows unique insights in epigenetic landscapes in different biological systems at single cell level.
Maintaining the stability of the housing market in the event of an economic shock
Authors: Stundziene Alina , Pilinkiene Vaida , Grybauskas Andrius 
Published: March 2022

Purpose
This paper aims to identify the economic stimulus measures that ensure stability of the Lithuanian housing market in the event of an economic shock.

Design/methodology/approach
The econometric analysis includes stationarity test, Granger causality test, correlation analysis, autoregressive distributed lag models and cointegration analysis using ARDL bounds testing.

Findings
The econometric modelling reveals that the housing price in Lithuania correlates with quarterly changes in the gross domestic product and approves that the cycles of the real estate market are related to the economic cycles. Economic stimulus measures should mainly focus on stabilizing the economics, preserving the cash and deposits of households, as well as consumer spending in the case of economic shock.

Originality Value
This study is beneficial for policy makers to make decisions to maintain stability in the housing market in the event of any economic shock.
Workplace spirituality, knowledge-hiding and the mediating role of organizational identification: evidence from Pakistan
Authors: Muavia Muhammad , Hussain Ghulam , Sahibzada Umar Farooq , Wan Ismail Wan Khairuzzaman 
Published: March 2022

PurposeThis research aims to investigate relationship between workplace spirituality and employees' knowledge-hiding as mediated by organizational identification.Design/methodology/approachIn this research, a time-lagged design is employed and multi-source data are collected through self-administered questionnaires. A sample of 305 focal respondents and 1,048 of the respondents' peers is used to test the hypotheses using AMOS (analysis of moment structures) 24.0.FindingsThe results reveal that two dimensions of workplace spirituality – meaningful work and values alignment –play significant roles in reducing knowledge-hiding in terms of workplace spirituality's three dimensions of evasive hiding, rationalized hiding and “playing dumb.” However, contrary to expectations, a sense of community has significant positive effects on the dimensions of knowledge-hiding. The study also reveals that organizational identification significantly mediates the relationships between the dimensions of workplace spirituality and those of knowledge-hiding.Originality/valueThis pioneer study introduces workplace spirituality (which differs from religious spirituality) and the significance of workplace spirituality in the workplace in the religious and conservative society of Pakistan. This study uses the lens of social identity theory (SIT) to establish for the first-time organizational identity as a mediating mechanism between workplace spirituality and knowledge-hiding to offer new insights for theory and practice.
An assessment tool for stakeholder integration excellence and project delivery optimisation
Authors: Ominde Diana , Ochieng Edward G. , Omwenga Vincent O. 
Published: March 2022

PurposeThe aim of this study was to appraise the delivery of information communication technology (ICT) projects and identify key determinants for stakeholder integration.Design/methodology/approachGiven that empirically, little was known about stakeholder integration in the ICT sector and its influence or effect on project delivery; qualitative method was used. Forty-seven semi-structured interviews were carried out to derive senior project practitioners and policymakers' constructs of stakeholder integration and infrastructure performance improvement of ICT projects. The verification and validation of the proposed assessment tool were achieved through the use of focus group discussion.FindingsAs established in this research study, there is a need for project delivery teams to evaluate the level of stakeholder integration, the formulation of a project business case, the project processes and issues of compliance and regulation in ICT projects. What is evident in the findings of the study is that the management model adopted for the stakeholders in the Kenyan ICT sector ought to make communication the fulcrum of their engagement.Originality/valueThe inferences made herein are critical in contributing to knowledge regarding the ICT infrastructure project management terrain in developing countries. There is evidence in the study to conclude that the concept of stakeholder management and integration has implications for the sustainability of ICT projects. One of the issues that predominantly featured in the research was the input of stakeholder integration in terms of project sustainability.
Spherical robot with spring energy storage type hopping mechanisms: design, dynamics and experimental evaluation
Authors: Dong Yangyang , Zhang Tongle , Han Shaojie , Guo Yipan , Zeng Bo , Wang Yongbin , Zhang Zijian 
Published: March 2022

Purpose
Spherical robot plays an essential role in the field of mobile robot because of its unique shape and omni-directional mobility, especially in the application of planet detection. Although spherical robot has many advantages over leg robot, its obstacle climbing performance is still not satisfactory, that is exactly the motivation of this paper. The purpose of this paper is to propose a high-performance hopping mechanism for spherical robot, which can adapt to different terrain and effectively cross obstacles.

Design/methodology/approach
The hopping system uses torque spring as part of the energy storage mechanism, and converts the kinetic energy of rotation into elastic potential energy with a particularly designed turntable. Moreover, the track of the turntable, based on the Archimedes spiral principle, has the attributes of equidistance and equivelocity that enable better stability of energy storage process.

Findings
Experiments show that the proposed hopping mechanism can make a 250 g spherical robot jump up to 58 cm with the take-off angle of 60°. Finally, the influence of friction and take-off angle on the hopping height and distance of the robot is also analyzed, which provides a prior guidance for optimizing its jumping process.

Originality/value
This paper shows how to easily design a lightweight, compact and embedded spring hopping structure so that a spherical hopping robot with detection ability can be developed.
Differential spillover effects of different non-audit fees on audit report lag
Authors: Lai Kam-Wah 
Published: March 2022

PurposeRegulators treat all non-audit services the same by using a broad-brush approach which is reflected in the study of total non-audit fees in the same analyses or different non-audit fees in isolation by prior studies. To know whether the non-audit services have different effects and hence, should be regulated separately, this paper compares their effects on audit report lag and examines whether they follow the implied hierarchy of the Securities and Exchange Commission.Design/methodology/approachThe effects of audit-related non-audit fees, tax fees and other non-audit fees are compared in an audit report lag model to determine whether they are the same statistically. Supporting tests for audit quality use discretionary accruals and the reporting of a small profit or small positive change in profit.FindingsThis paper finds that different non-audit fees do not have the same effects on report lag and partial support for the implied hierarchy of the Commission. Specifically, for large accelerated filers, audit-related fees and tax fees have the same negative effects on report lag but other non-audit fees are unrelated to report lag. Tests of audit quality suggest that auditors do not compromise audit quality.Research limitations/implicationsDifferent non-audit services are unique in their spillover effects and deserve individual attention. Audit practitioners could be more comfortable in providing audit-related non-audit or tax services for audit clients since these services could facilitate audit work without compromising independence. On the other hand, they should be cautious about the provision of other non-audit services because the services do not enhance the efficiency of audit work and without such a benefit to audit clients, the provision may create issues of perceived independence.Practical implicationsInsight is limited by the types of disclosure of non-audit fees available and the lack of internal measures of audit efficiency.Originality/valueThe results provide deeper insight into the knowledge spillover theory and prior studies which implicitly assume all non-audit services having the same effect. The results suggest that the services should be regulated each on its own but not in a bundle. Last, this paper provides the first evidence that audit-related non-audit fees reduce report lag.
Information experience as an object of LIS research: a definition based on concept analysis
Authors: Yu Liangzhi , Liu Yijun 
Published: March 2022

PurposeThis study aims to contribute to the clarification of core concepts in information experience research and to the consolidation of information experience as a distinctive research object.Design/methodology/approachThis study adopts a series of techniques from Wilson's toolkit of concept analysis.FindingsThis study finds that there exist tensions between different uses of the term information experience, giving rise to two fundamentally different conceptions of this particular human experience which this study names, respectively, the posterior conception and the a priori conception. It also finds that it is linguistically more useful, practically more consonant with LIS's concerns and unitarily more consistent to define information experience following the a priori conception. It postulates that information experience can be defined as a person's subjective, pre-reflective living through of his/her life as an information user in the information sphere of the lifeworld.Research limitations/implicationsIf adopted by future research, the concept proposed in this study is likely to push information experience research toward a more prominent phenomenological turn on the one hand, and a return to conventional LIS concerns on the other.Practical implicationsThe clarified concept may help user experience librarians and system designers to see the relevance of information experience research for their work more clearly.Originality/valueBy identifying, comparing and discussing different existing uses of information experience, and by suggesting a redefinition of the concept, this study has brought the core concepts of information experience research to a new level of clarity, and has verified information experience as a distinctive object for LIS research.
Predicting purchase behaviour of Indonesian and French Muslim consumers: insights from a multi-group analysis
Authors: Firdaus Farah Syahida , Ikhsan Ridho Bramulya , Fernando Yudi 
Published: March 2022

Purpose
This paper aims to model Muslim consumers' purchase behaviour that predicts the impacts of behavioural factors of spirituality, emotional value, image, trust and satisfaction on Halal-labelled food products. The model was used among Muslim consumers in Indonesia and France.

Design/methodology/approach
A survey was conducted among Indonesian and French Muslim consumers who had bought Halal-labelled food products. The model was examined using Partial Least Squares Structural Equation Modeling (PLS-SEM) with multi-group analysis (PLS-MGA) to test specific differences between sample groups.

Findings
All proposed hypotheses were accepted, except for the trust in purchasing behaviour. It was not significantly different in the two sample groups. The linkage from image to purchasing behaviour was not significantly related to the French sample group, and emotional value did not influence Halal-labelled food product purchase behaviour in the Indonesian sample group. The MGA results found a significant difference in spirituality, emotional value image and trust among Indonesian and French Muslim consumers.

Practical implications
The guarantee of Halal food through a Halal label can fulfil the spirituality of Muslim consumers in carrying out Allah’s (SWT) command to consume Halal food, creating a product image, trust, satisfaction and emotional value that encourages positive buying behaviour. The finding shows that Muslim spirituality has extended the Islamic marketing literature to predict Muslim consumer behaviour. The company can emphasise in advertisements that the Halal-certified logo reflects the quality of products.

Originality/value
To the best of the authors’ knowledge, this research is among the early study empirically confirming that spirituality and emotional value are critical domains to predict purchase behaviour between two different groups of Indonesian and French Muslim consumers.
Emission and sectoral energy intensity: a variance decomposition analysis
Authors: Mukhopadhyay Ujjaini , Pani Ratnakar 
Published: March 2022

PurposeIn the backdrop of growing global concern on escalating CO2 emission leading to climate disorder and controversy between economic growth and environment, this study undertakes a decomposition analysis of the top 20 emitters of the world during 1992–2016 with two objectives: to identify the relative contribution of the major driving factors in CO2 emission and to comprehend the role and performance of sectoral energy consumption pattern in changing the emission level.Design/methodology/approachThe paper uses variance analysis method to perform two stage decomposition: first, it decomposes emission into the major driving factors, and, secondly, it also decomposes fossil fuel intensity of different sectors into fuel mix and energy intensity effects, which are new in the literature.FindingsThe results indicate that aggressive pursuit for economic growth, particularly by developing countries, is the major reason behind unprecedented emission growth, with income effect, fossil fuel intensity effect and population effect having substantial roles. Considerable decline in dependence on fossil fuel, coupled with rising emissions, signifies that emission intensity is still to be harnessed. Sectoral decomposition shows that while fossil fuel intensity has declined in residential sector, it has remarkably shot up in industry, transport and commercial sectors. On the other hand, sectoral energy intensity has declined, particularly due to favourable performances of transport and commercial sectors.Research limitations/implicationsThe detailed country-wise sectoral analysis identifies the sectors with favourable contribution in curtailing emission and lends a direction to other countries for policy making.Originality/valueThis study contributes by incorporating multi-country sectoral segregation in decomposition analysis. It focuses not only on energy intensity, but on the effect of energy substitution in each sector as well. It identifies the sectors that have lowered their dependence on fossil fuel to highlight that emission can effectively be dealt with through a prudent choice of fuel mix.
Administrators within personality disorder services: their role and contribution in maintaining the overall structure and treatment approach
Authors: Larsson-Thomas Emma , Ruprai Sukhi , Manonga Louise , Lee Tennyson 
Published: March 2022

Purpose
People with personality disorders often present with interpersonal difficulties which affect their relationship with significant others but also with staff involved in their care. Administrators work in “frontline positions” where they are required to face challenging situations yet their role has not been studied. This study aims to describe the role and contribution of an administrator in a personality disorder service.

Design/methodology/approach
A mixed-methods design was used. All incoming calls to a specialist personality disorder service over three months were documented. A semi-structured focus group (n = 7) with clinicians working in the service was conducted. The data was analysed using thematic analysis. Clinical vignettes are presented to highlight typical interactions.

Findings
The qualitative results highlighted that the administrator is key in psychological preparations, managing pressure and maintaining clinical boundaries. Traits identified as useful in an administrator working in a personality disorder service are flexibility, consistency and assertiveness. Tensions between administrators and clinicians were related to the role definition of the administrator, boundaries, countertransference and process interaction. The majority of incoming calls were from patients scheduling and cancelling appointments. Only 3% of calls evoked negative feelings in the administrator such as feeling “annoyed” or “drained”.

Practical implications
Results highlight a need for careful selection, training and supervision of staff. A key recommendation is the need for integration and close coordination of the administrator within the clinical team.

Originality/value
This study represents one of the first efforts to explore the contribution of administrators within personality disorder services. It explores the impact of the administrator on the team.
Severe skin inflammation leads to salivary gland atrophy and dysfunction
Authors: Matsushima Yoshiaki , Mizutani Kento , Iida Shohei , Ichishi Masako , Nakanishi Takehisa , Okada Karin , Umaoka Ai , Kondo Makoto , Habe Koji , Watanabe Masatoshi , Yamanaka Keiichi 
Published: March 2022

AbstractPsoriasis and atopic dermatitis are inflammatory skin diseases, and these patients have an increased risk of cardiovascular events and other medical complications. It has been clarified that skin inflammation affects internal organs. Additionally, dental caries tends to occur more frequently in patients with psoriasis and atopic dermatitis. In this study, we aim to investigate the effects of dermatitis on the salivary glands using an inflammation model mouse. Salivary secretion stimulated with pilocarpine was reduced in dermatitis mice. Histologically, dermatitis mice showed amyloid deposition, glandular atrophy, and fibrosis in the salivary glands. Expression of inflammatory cytokines in the salivary glands was higher in dermatitis mice; however, secretion of cytokines in saliva was not significantly different. Dermatitis mice showed decreased salivary secretion and histological changes, which may cause periodontal disease. Therefore, appropriate control of skin inflammation is essential.
Ethical public typology: How does moral foundation theory and anticorporatism predict changes in public perceptions during a crisis?
Authors: Hong Seoyeon , Shim Kyujin 
Published: March 2022

AbstractThis study proposes a new public typology utilising moral foundation theory and anticorporatism. The current paper conceptualised four ethical types of public (e.g., moralists, antagonists, optimists, and pragmatists) and conducted an online survey using a national sample (N = 1124) to test the applicability of the new typology. Our results suggest that different types of publics react differently in attributing crisis responsibility, expressing their emotional responses, and showing boycott intentions in evaluating a corporate crisis.