bluuebunny/crossref_metadata_2025_split huggingface dataset:
H3K36 demethylase JMJ710 negatively regulates drought tolerance by suppressing MYB48-1 expression in rice
Authors: Zhao Weijie , Wang Xiaoyan , Zhang Qian , Zheng Qian , Yao Haitao , Gu Xiangyang , Liu Dongliang , Tian Xuemin , Wang Xiaoji , Li Yongqing , Zhu Zhengge 
Published: March 2022

Abstract
The homeostasis of histone methylation is maintained by histone methyltransferases and demethylases, which are important for the regulation of gene expression. Here, we report a histone demethylase from rice (Oryza sativa), Jumonji C domain-containing protein (JMJ710), which belongs to the JMJD6 group and plays an important role in the response to drought stress. Overexpression of JMJ710 causes a drought-sensitive phenotype, while RNAi and clustered regularly interspaced short palindromic repeats (CRISPR)-knockout mutant lines show drought tolerance. In vitro and in vivo assays showed that JMJ710 is a histone demethylase. It targets to MYB TRANSCRIPTION FACTOR 48 (MYB48-1) chromatin, demethylates H3K36me2, and negatively regulates the expression of MYB48-1, a positive regulator of drought tolerance. Under drought stress, JMJ710 is downregulated and the expression of MYB48-1 increases, and the subsequent activation of its downstream drought-responsive genes leads to drought tolerance. This research reports a negative regulator of drought stress-responsive genes, JMJ710, that ensures that the drought tolerance mechanism is not mis-activated under normal conditions but allows quick activation upon drought stress.
Ready for a Woman President?
Authors: DeMora Stephanie L , Lindke Christian A , Merolla Jennifer L , Stephenson Laura B 
Published: March 2022

AbstractEven though a record number of women ran for the Democratic nomination in 2020, Clinton’s loss in 2016 led pundits, party elites, and voters to worry about whether the country would be willing to support a woman for president, and polling organizations regularly asked questions that tapped into such concerns. While the vast majority expressed willingness to vote for a woman for president in polls, people were more skeptical about how their neighbors felt. Our research question cuts to the heart of this issue: How does polling information about comfort with the idea of a woman president affect perceptions of the electability of actual women running for their party’s nomination, and in turn voting decisions? We expect that exposure to signals of low comfort with a woman president will reduce perceptions of electability, and in turn dampen support for women at the nomination stage, but there are competing hypotheses for how signals of high comfort will be received. We further expect that Democratic women will be most affected by such information. We test these expectations with an experiment fielded on the 2019 Cooperative Congressional Election Study (CCES). Our findings have important implications for media coverage of polls related to women running for executive office.
Climate Change Risk and the Cost of Mortgage Credit
Authors: Nguyen Duc Duy , Ongena Steven , Qi Shusen , Sila Vathunyoo 
Published: March 2022

Abstract
We show that lenders charge higher interest rates for mortgages on properties exposed to a greater risk of sea level rise (SLR). This SLR premium is not evident in short-term loans and is not related to borrowers’ short-term realized default or creditworthiness. Further, the SLR premium is smaller when the consequences of climate change are less salient and in areas with more climate change deniers. Overall, our results suggest that mortgage lenders view the risk of SLR as a long-term risk and that attention and beliefs are potential barriers through which SLR risk is priced in residential mortgage markets.
Preferred policy responses to technological change: Survey evidence from OECD countries
Authors: Busemeyer Marius R , Gandenberger Mia , Knotz Carlo , Tober Tobias 
Published: March 2022

AbstractHow do the labor market risks associated with technological change affect policy preferences? We argue that higher perceptions of technology-related risks should increase support for compensation and decrease support for social investment. We expect the opposite effect for individuals who use technology constantly at work, have a university degree and earn higher incomes. However, as the perception of technology-related employment risks in the latter group of individuals increases, so does their preference for compensatory and protective policy solutions to technological change. Our expectations are confirmed by novel data from a survey of 24 diverse Organisation for Economic Co-operation and Development (OECD) countries that includes specifically designed questions on technology-related risks and policy preferences. The results suggest that technology-related risks not only correlate with certain demographic and occupational characteristics, but also cross-cut them. Thus, technology-related risks might not only become a source of new cleavages between the losers and winners of technological change, but also the basis for new cross-class coalitions.
Novel Pedicle Navigator Based on Micro Inertial Navigation System (MINS) and Bioelectric Impedance Analysis (BIA) to Facilitate Pedicle Screw Placement in Spine Surgery: Study in a Porcine Model
Authors: Lin Wentao , Xie Faqin , Zhao Shuofeng , Lin Songhui , He Chaoqin , Wang Zhiyun 
Published: March 2022

Study Design.
A porcine model.

Objective.
The study aims to design a novel pedicle navigator based on micro-inertial navigation system (MINS) and bioelectrical impedance analysis (BIA) to assist place pedicle screw placement and validate the utility of the system in enhancing pedicle screw placement.

Summary of Background Data.
The incidence of pedicle screw malpositioning in complicated spinal surgery is still high.
Procedures such as computed tomography image-guided navigation, and robot-assisted surgery have been used to improve the precision of pedicle screw placement, but it remains an unmet clinical need.

Methods.
The miniaturized integrated framework containing MINS was mounted inside the hollow handle of the pedicle finder. The inner core was complemented by a high-intensity electrode for measuring bioelectric impedance. Twelve healthy male Wuzhishan minipigs of similar age and weight were used in this experiment and randomized to the MINS-BIA or freehand (FH) group. Pedicle screw placement was determined according to the modified Gertzbein–Robbins grading system on computed tomography images. An impedance detected by probe equal to the baseline value for soft tissue was defined as cortical bone perforation.

Results.
A total of 216 screws were placed in 12 minipigs. There were 15 pedicle breaches in the navigator group and 31 in the FH group; the detection rates of these breaches were 14 of 15 (93.3%) and 25 of 31 (80.6%), respectively, with a statistically significant difference between groups. The mean offsets between the planned and postoperatively measured tilt angles of the screw trajectory were 4.5° ± 5.5° in the axial plane and 4.8° ± 3.3° in the sagittal plane with the navigator system and 7.0° ± 5.1° and 7.7° ± 4.7°, respectively, with the FH technique; the differences were statistically significant.

Conclusion.
A novel and portable navigator based on MINS and BIA could be beneficial for improving or maintaining accuracy while reducing overall radiation exposure.
Management of prostatosymphyseal fistula following photoselective vaporization of the prostate: Case series and systematic review of the literature
Authors: Lundy Scott D. , Hauser Nicholas , Wood Hadley , Fergany Amr , Angermeier Kenneth 
Published: March 2022

Abstract

Background:
This study is aimed to describe our institutional experience and review the literature to date on prostatosymphyseal fistula (PSF), or puboprostatic fistula, following bladder outlet procedures such as transurethral resection of the prostate (TURP) or laser photoselective vaporization of the prostate (PVP).

Materials and methods:
We retrospectively queried our institutional experience for management of PSF following PVP performed for symptomatic benign prostatic hyperplasia. We also performed a systematic literature review for PSF following PVP or TURP. Finally, we describe our surgical approach to the management of this challenging condition.

Results:
We identified 7 cases of PSF following PVP from our institution, as well as an additional 7 cases following PVP and 9 cases following TURP from literature review. The diagnosis of PSF was made between 0.5 and 24 months following PVP, and the most specific symptoms were pubic pain and difficulty ambulating. Most patients requiring several evaluations before the diagnosis was made using appropriate imaging studies. Seventy percent of patients required surgical intervention including fistula repair or prostatectomy. Our surgical approach has evolved, and we now routinely perform robotic fistula repair with Y-V plasty and interposition flap with excellent results.

Conclusions:
Puboprostatic fistula is a rare and poorly described complication of PVP or TURP. To the best of our knowledge, this case series of PSF following PVP represents the largest series to date and doubles the number of reported cases in the literature. Robotic fistula repair with interposition of either peritoneal or perivesical fat flaps appears to be a viable management strategy.
Influences of achieved SBP on age and sex-related left ventricular structural alteration in community hypertensive populations
Authors: Cai Anping , Liu Lin , Zhou Dan , Tang Songtao , Zhou Yingling , Feng Yingqing 
Published: March 2022

Objective:
To evaluate age and sex-related left ventricular (LV) structural alteration in community hypertensive populations, and to explore whether achieved systolic blood pressure (SBP) (< 120, < 130 and <140 mmHg) would modify the association between age and sex with LV structural alteration.

Method:
A total of 1419 community hypertensive people (mean age 61 years, women 50.5%) without cardiovascular disease and left ventricular hypertrophy (LVH) at baseline were included. LV structural measures were assessed using transthoracic echocardiography at baseline and at follow-up. Age and sex-related LV structural alteration was evaluated using linear and Cox regression analysis as appropriate.

Results:
Per 10 years increase in age was associated with positive alteration of LV mass (LVM) index (coefficient-β: 2.63) and LV mass-to-volume (LVMV) ratio (coefficient-β: 0.04). Female sex was associated with positive alteration of LVM index (coefficient-β: 4.87) and LVMV ratio (coefficient-β: 0.05). After a median follow-up of 4years, compared to men, women had a higher rate of LVH (36.8% vs. 11.5%, P
< 0.001). The association between age and incident LVH was nonsignificant when achieved SBP <120 mmHg. The association between female sex and LVM index alteration was nonsignificant when achieved SBP <120mmHg and <130mmHg.

Conclusion:
Age and female sex were associated with a phenotype of LV remodeling which was featured by increased LVM index and concentric remodeling. Women had a higher risk of developing LVH. A lower achieved SBP conferred greater effects on mitigating age and sex-related LV structural alteration.

Video abstract:

http://links.lww.com/HJH/B907
.
CENTRAL RETINAL ARTERY OCCLUSION AS PRESENTATION OF BARTONELLA ENDOCARDITIS
Authors: Chebolu Apoorv P. , Wallsh Josh O. , Falk Naomi , Bhatnagar Pawan 
Published: March 2022

Background/Purpose:
To describe a rare ocular presentation of a systemic illness and the important lifesaving diagnosis made by a complete eye examination.

Methods:
The patient was evaluated with a comprehensive ophthalmic examination and followed closely in the outpatient setting with optical coherence tomography, fluorescein angiography, and color fundus photos.

Results:
A 66-year-old man presented with acute vision loss of the left eye. A complete eye examination revealed that he had a central retinal artery occlusion. Systemic workup revealed that he had a mitral valve vegetation and blood cultures grew Bartonella henselae. His kidney biopsy showed membranoproliferative glomerulonephritis, which is often seen with septic emboli. Furthermore, the patient lacked any ocular inflammatory signs. This constellation of findings was diagnostic for a thromboembolic etiology causing his central retinal artery occlusion. At follow-up, the optical coherence tomography demonstrated inner retinal hyperreflectivity and the fluorescein angiogram showed segmented flow and no neovascularization. On follow-up, the patient had a stable examination with light perception vision and completed antibiotic therapy for bartonella endocarditis.

Conclusion:
The detection of a fatal systemic illness was made promptly with a thorough ocular examination. We highlight the importance of a multidisciplinary approach in making a lifesaving diagnosis.
Occlusive retinal vasculitis: novel insights into causes, pathogenesis and treatment
Authors: Arepalli Sruthi R. , Thomas Akshay S. 
Published: March 2022

Purpose of review
Occlusive retinal vasculitis (ORV) has a large differential diagnosis and varied therapeutic approaches. This review highlights existing and novel causes and treatment options for ORV.

Recent findings
Mutations in CAPN5, TREX1, and TNFAIP3 have been associated with dominantly inherited forms of ORV. Various intraocular therapeutics have been shown to occasionally cause ORV; the most recent medications associated with ORV are vancomycin and brolucizumab. In cases of ORV linked to Behçet's disease, clinical trials support the use of tumor necrosis factor alpha inhibitors.

Summary
Identification of the underlying etiology of ORV is critical to help guide treatment. Treatment in ORV involves both treatment any underlying infection or autoimmune condition, cessation of the any offending causative agent and local treatment of neovascular complications.
Ophthalmologists' attitudes toward immediate sequential bilateral cataract surgery: Dutch national survey
Authors: Spekreijse Lindsay S. , Veldhuizen Claudette A. , Henry Ype P. , van den Biggelaar Frank J.H.M. , Dirksen Carmen D. , Nuijts Rudy M.M.A. 
Published: March 2022

Purpose:
To evaluate current practice patterns of immediate sequential bilateral cataract surgery (ISBCS) in the Netherlands and assess ophthalmologists' attitudes toward performing ISBCS in future cataract care.

Setting:
Dutch ophthalmic society members.

Design:
Cross-sectional study (national survey).

Methods:
An electronic survey on ISBCS was sent as part of an annual survey on cataract practice patterns to members of the Dutch ophthalmic society. Questions regarding current ISBCS practice patterns, willingness to perform ISBCS routinely in future care, reasons for performing ISBCS, and reasons for not performing ISBCS were included. Data were analyzed using descriptive statistics.

Results:
237 (45.6%) of 520 survey recipients responded to the overall survey. Data on the ISBCS questions were available from 227 respondents. 62 ophthalmologists (27.3%) currently performed ISBCS, predominantly in low patient volumes (90.3% on 1 to 5 patients per month). However, 108 (47.6%) of 227 ophthalmologists considered performing ISBCS routinely in future practice. Procedures for which ISBCS was mainly considered included age-related cataract surgery using topical and general anesthesia. Availability of separate products and instruments for both eyes and patient advantages were considered of high importance when performing ISBCS. Main reasons for not performing ISBCS included the risk for endophthalmitis and potential medicolegal aspects.

Conclusions:
Although ISBCS is currently not a routine procedure in the Netherlands, it is considered by almost 50% of surgeons. To improve implementation on a national level, potential barriers identified in this survey (fear of bilateral endophthalmitis, potential medicolegal issues, and a lack of availability of separate products for both eyes) should be addressed.
To Use the Back-up Cochlear Implant or Not? Using Intra-operative Impedance to Guide Your Decisions
Authors: Mehta Nishchay , Chu Philip , Birman Catherine S. 
Published: March 2022

Introduction:
Intra-operative electrophysiological testing is being increasingly used to determine device functionality. Impedance abnormalities (open or short circuits) measured at time of surgery pose a dilemma: is it likely to resolve or is it a permanent fault? There is little in the literature on how to manage these intraoperative finding and if, at time of surgery, the back-up device should be used.

Methods:
We routinely undertake impedance testing twice intraoperatively, as well as at switch on, 1 and 3 months postoperatively. Retrospective impedance thresholds were analysed for one surgeon's cases between January 2018 and December 2019.

Results:
There were 235 cochlear implants performed for 217 patients (5,020 electrode contacts) analysed. Thirty-three electrodes had abnormal impedance thresholds on first intraoperative cycle of testing, 76% resolving with the second testing cycle electrode contacts that demonstrated abnormal impedance during both intraoperative test cycles were 16.54 times (95%CI 2.55–107.13, p = 0.003) more likely to be abnormal at three months. Fifty percent resolved by switch on. The intraoperative abnormalities made up 26% of electrode abnormalities seen at 3 months postoperatively.

Discussion:
This study demonstrates the utility of 2 cycles of intraoperative impedance testing, with persistently abnormal electrodes having 16 times the likelihood of persistent abnormalities of impedance, and 50% resolution. These persistent intra-operative abnormal electrodes are responsibly for 26% of electrode abnormalities at 3 months. This information is useful for the surgeon when considering use of the backup cochlear implant device.
Optical Clearing Agents for Optical Imaging Through Cartilage Tympanoplasties: A Preclinical Feasibility Study
Authors: Wang Junzhe , Chawdhary Gaurav , Yang Xiaojie , Morin Francis , Khalid-Raja Mamoona , Farrell Joshua , MacDougall Dan , Chen Fangyi , Morris David P. , Adamson Robert B. A. 
Published: March 2022

Hypothesis:
Optical clearing agents (OCAs) can render cartilage tympanoplasty grafts sufficiently transparent to permit visualization of middle ear structures in an operated ear using optical coherence tomography (OCT) imaging.

Methods:
Pieces of human tragal cartilage were treated with glycerol, a commonly used OCA. A reference reflector was imaged with OCT through the tympanoplasty as it cleared and the optical attenuation of the graft was measured. The reversibility of clearing and the dimensional changes associated with glycerol absorption were also measured. In a separate experiment, a human cadaveric temporal bone was prepared to simulate an ossiculoplasty surgery with cartilage replacement of the tympanic membrane. A partial ossicular replacement prosthesis (PORP) inserted in the ear was imaged with OCT through a 0.4mm cartilage graft optically cleared with glycerol.

Main Outcome Measure:
The optical attenuation of 0.4mm cartilage grafts decreased at 2.3+/−1.1 dB/min following treatment with glycerol, reaching a total decrease in attenuation of 13.6+/−5.9 dB after 7 minutes. The optical and dimensional effects of glycerol absorption were reversable following saline washout. In the temporal bone preparation, treatment of a cartilage graft with glycerol resulted in a 13 dB increase in signal-to-noise ratio and a 13 dB increase in contrast for visualizing the PORP through the graft with OCT.

Conclusions:
Optical clearing agents offer a potential pathway towards optical coherence tomography imaging of the middle ear in post-surgical ears with cartilage grafts.
Extracorporeal Membrane Oxygenation as a Bridge to Surgical Repair of Postinfarct Ventricular Septal Defect
Authors: Doi Atsuo , Negri Justin C. , Marasco Silvana F. , Gooi Julian H. , Zimmet Adam , Pellegrino Vincent A. , Nanjayya Vinodh B. , McGloughlin Steve A. , McGiffin David C. 
Published: March 2022

Postinfarct ventricular septal defect (PIVSD) is associated with high mortality and the management of these patients has been a challenge with little improvement in outcomes. We commenced a protocol of veno-arterial extracorporeal membrane oxygenation (VA ECMO) for those patients who present in cardiogenic shock with the aim to improve end-organ function before definitive surgical repair to reduce postoperative mortality. This study reviewed the results of this strategy. This was a single-center, retrospective review of all patients who were admitted to our institution with PIVSD in cardiogenic shock from September 2015 to November 2019. Clinical and investigative data were evaluated. Eight patients were referred with PIVSD during this period in cardiogenic shock. One patient had an anterior PIVSD and the other seven had inferior PIVSD. Six patients underwent surgical repair at a median (interquartile range, IQR) of 7 (5–8) days after initiation of VA ECMO. Two patients did not undergo surgical repair. Five patients survived after surgery and one patient died postoperatively due to multiorgan failure.
Preoperative use of VA ECMO is a feasible strategy for PIVSD and may improve the results of repair.
Cone-Beam Computed Tomography-Based Spatial Prediction of Drug Dose After Transarterial Chemoembolization Using Radiopaque Drug-Eluting Beads in Woodchuck Hepatocellular Carcinoma
Authors: Mikhail Andrew S. , Pritchard William F. , Negussie Ayele H. , Inkiyad Gazi , Long Dilara J. , Mauda-Havakuk Michal , Wakim Paul G. , van der Sterren William , Levy Elliot B. , Lewis Andrew L. , Karanian John W. , Wood Bradford J. 
Published: March 2022

Objectives
The aims of this study were to develop a model to estimate drug dose delivered to tumors after transarterial chemoembolization (TACE) with radiopaque drug-eluting beads (DEBs) based on DEB density on cone-beam computed tomography (CT) and to evaluate drug penetration into tissue in a woodchuck hepatoma model.

Materials and Methods
Transarterial chemoembolization was performed in woodchucks with hepatocellular carcinoma (N = 5) using DEBs (70–150 μm, LC Bead LUMI) loaded with doxorubicin. Livers were resected 45 minutes after embolization, immediately frozen, and cut using liver-specific, 3D-printed sectioning molds. Doxorubicin levels in tumor specimens were measured by high-performance liquid chromatography and correlated with DEB iodine content that was measured using prototype cone-beam CT-based embolization treatment planning software. Doxorubicin penetration into tissue surrounding DEBs was assessed by fluorescence microscopy of tumor sections. Fluorescence intensity was converted into doxorubicin concentration using calibration standards. Intensity-thresholded color heatmaps were generated representing extravascular drug penetration.

Results
Consistent segmentation of DEBs on cone-beam CT was achieved using a semiautomated intensity thresholding method. A positive linear correlation (0.96) was found between DEB iodine content measured on cone-beam CT and the amount of doxorubicin measured in tumor specimens. Prediction of doxorubicin levels in tumor sections that were not included in model development was accurate, with a root-mean-square error of 0.08 mg of doxorubicin. Tumor penetration of eluted doxorubicin resulted in concentration gradients where drug content decreased with increasing distance from blood vessels containing DEBs. Drug penetration was greater for blood vessels containing DEB clusters compared with single DEB, with higher doxorubicin concentrations extending further away from the vessels.

Conclusions
Estimation of drug dose delivered during transarterial chemoembolization in a woodchuck hepatocellular carcinoma model was possible using DEB radiopacity on cone-beam CT as a surrogate marker. Doxorubicin penetration was greatest adjacent to vessels containing DEB clusters compared with single DEB. Intraprocedural estimation of the spatial distribution of drug dose within the tumor could enable real-time adjustments to DEB delivery, to maximize treatment coverage or identify regions of tumor at risk for undertreatment.