bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between neonatal uterine bleeding and endometriosis-related symptoms later in life
Authors: Ogawa Kanae , Khan Khaleque N , Kuroboshi Haruo , Koshiba Akemi , Horiguchi Go , Teramukai Satoshi , Fujishita Akira , Itoh Kyoko , Kitawaki Jo , Mori Taisuke 
Published: August 2022

Abstract
Background: Neonatal uterine bleeding (NUB) is reported to be involved in early onset endometriosis. There is no information whether young women with a history of NUB may manifest various symptoms related to endometriosis. In the present study we investigated the possible association between NUB and endometriosis-related symptoms later in life who are now adults. Methods: This is a retrospective case-controlled cohort study and prospective evaluation of questionnaire-based symptoms related to endometriosis among young women born with and without NUB. The prevalence of NUB was calculated and multiple logistic regression analysis with variable confounding factors was conducted to identify risk factors of NUB. The results of web-based questionnaire survey were analyzed by continuous variables using Wilcoxon rank sum test and categorical variables using Fisher’s exact test to compare endometriosis-related symptoms. Results: Among 807 female babies born at our hospital between 2013 and 2017, NUB occurred in 25 cases with a prevalence of 3.1%. Twenty-five NUB cases showed overt bleeding 4.5±1.8 days after birth (median 4 days, range 1-8 days) and it lasted for 1.4±0.8 days (median 1 day, range 1-4 days). Multiple logistic regression analysis indicated that younger age of the mother ((OR, 0.92, 95% CI 0.85-1.00, P=0.048)) and longer gestational age of 39 weeks (OR 3.04, 95% CI 1.43-6.45, P=0.004) and ≥40 weeks (OR 4.54, 95% CI 2.20-9.39, P<0.0001) of gestation were significantly associated with the occurrence of NUB. Young women with or without a history of NUB during the period of 1996-2000 manifested various endometriosis-related symptoms.Conclusion: We confirmed the validity of the reported prevalence and risk factors of NUB. Young mothers and longer gestational age are the risk factors of NUB and newborn females with or without NUB similarly suffer from various endometriosis-related symptoms later in life. Future prospective cross-sectional study is warranted to confirm the endometriosis-related symptoms in NUB+ cases by physical examination/ultrasound/MRI or laparoscopy. Trial registration: not applicable
Plant-Extract-Assisted Eco-friendly synthesis of Iron oxide nanoparticles using cape gooseberry Extract and their Antibacterial study
Authors: Birusanti Arun Babu , Espenti Chandra Sekhar , Mala Sreenivasulu 
Published: August 2022

Abstract
Cape gooseberry plant leaf extract mediated iron oxide nanoparticles (CG-IONPs) were prepared for the antibacterial study. The optimum precursor salt concentration, pH of the reaction mixture, the ratio between reducing agent and precursor salt, and time for the synthesis of iron nanoparticles were found to be 5 mM, 9.0, 3:7, and 0th, respectively. The synthesized iron nanoparticles were characterized by UV/Vis absorption spectroscopy, Photoluminescence Spectroscopy (PL), Transmission Electron Microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Thermal Analysis (TG/DTA), and Fourier transform infrared spectroscopy (FTIR). A FTIR measurement was carried out to identify the possible molecules like carbonyl, CH, and OH bands which were responsible for the reduction and formation of the nanoparticles. From the X-ray diffraction (XRD) method, it was found that the average particle size of magnetite nanoparticles was found to be 24.18 nm. TEM analysis has been carried out to determine the size and shape of the synthesized CG-IONPs. The thermal stability of the synthesized CG-IONPs was tested by performing TG/DTA analysis, using a nitrogen atmosphere. XPS analysis was carried out to measure the elemental composition and oxidation states of the elements present in CG-IONPs. The PL analysis has been employed in the present study to explore the luminescent character of CG-IONPs. The synthesized CG-IONPs had antibacterial activity against pathogenic bacteria like Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli, and Salmonella enterica by the well diffusion method. This biosynthesis method has been found to be cost-effective, good for the environment, and likely to be useful in many fields.
Metabarcoding Inventory of an Arctic Tundra Soil Ecosystem Reveals Highly Endemic Communities
Authors: Jorna Jesse , Vandenbrink Bryan , Hogg Ian D. , Wall Diana H. , Adams Byron J. 
Published: August 2022

Abstract
Biodiversity surveys of Arctic soil ecosystems are limited. Here, we provide a sequence-based inventory of soil fauna from an Arctic tundra ecosystem near Iqaluktuutiaq (Cambridge Bay), Nunavut. Invertebrate communities were extracted from soil at three sites with vegetation cover and three non-vegetated sites and inventoried using 18S metabarcode sequencing. A total of 361 Amplicon Sequence Variants (ASV) were identified and assigned to the closest matching taxonomic orders, most of which belonged to the Nematoda and Arthropoda. Vegetated soils showed no significantly higher ASV richness relative to non-vegetated soils although they contained a significantly higher diversity of arthropod taxa including insects, mites and springtails. The majority of taxa were found only at a single location and communities were distinct between soils even for sites with vegetation cover, suggesting that belowground species richness in Arctic tundra habitats is highly endemic and heterogeneous. Preserving endemicity is essential for Inuit communities who rely on intact tundra ecosystems for their health and wellbeing.
Handgrip strength conditional tolerance regions suggest the need for personalized sarcopenia definition: An analysis of the American NHANES database
Authors: Matabuena Marcos , Abdalla Pedro Pugliesi , Machado Dalmo Roberto Lopes , Bohn Lucimere , Mota Jorge 
Published: August 2022

Abstract
Background : Handgrip strength (HGS) is a well-established clinical biomarker that assesses functional capacity in older populations. In addition, HGS is a diagnostic tool that forecasts aging health conditions, such as sarcopenia. Aims : This paper provides HGS statistical tolerance regions and presents the need to establish HGS reference values according to patients' characteristics. Methods : For this purpose, we used a conditional tolerance algorithm for HGS, and we observed the tolerances regions in different age strata and sex of non-sarcopenic individuals from the National Health and Nutrition Examination Survey (NHANES, wave 2011-2012). Results and Discussion : Our results have critical implications for sarcopenia since conventional and available HGS cut-offs do not consider age range. Conclusions : This paper offers new perspectives on the evolution of traditional definitions of sarcopenia according to the principles of precision medicine.
Application of weather research and forecasting (WRF) model in evaluating depth–duration–frequency estimates under a future climate scenario
Authors: Mohebbi Amin , Adams Katlynn Rose , Akbariyeh Simin , Maruf Montasir , Hosseini Baghanam Aida 
Published: August 2022

Abstract
Point precipitation frequency estimates (PPFEs) are used in the design of stormwater drainage infrastructures. The PPFEs for 1-year to 1,000-year recurrence intervals with durations ranging from 5 min to 60 days are published in the National Oceanic and Atmospheric Administration (NOAA) Atlas 14. NOAA's published PPFEs are accepted as a reliable resource for the design of urban drainage infrastructures, yet they are based on the stationary climate assumption. However, future climate change may affect the distribution, frequency, and intensity of precipitation events. To evaluate the potential impacts of climate change on the stationary climate assumption, a weather research and forecasting (WRF) model was developed based on the medium climate change emission scenario. Then, the PPFEs were determined for 2010–2015, 2030–2035, 2050–2055, and 2070–2075 at six different locations in the state of Arizona. The comparison of the published and simulated PPFEs revealed a varying trend in depth and frequency values dependent on location and climate. Additionally, depth values were diminished for &lt;3-h events at a majority of the stations.
Modern vision for critical ﬂow in an egg-shaped section (Part II)
Authors: Soraya Bouslah , Lamia Hachemi-Rachedi , Moussa Lakehal , Bachir Achour 
Published: August 2022

Abstract
The main aim of this research was to examine the simultaneous effects of all flow parameters on the critical regime occurrence frequency in an egg-shaped conduit. Furthermore, one may first study the impacts of the conduit's generator diameter, then the influence of the generator diameter/longitudinal slope, and finally the effect of all parameters: the generator diameter, longitudinal slope, roughness and viscosity. It has been concluded that all these parameters, combined, have limit values that can generate a single critical state from which a diagram can be drawn showing different states of the critical flow that can be observed. Notably, this diagram is established according to the following practical ranges: relative roughness 0 ≤ ε/D ≤ 5.10−2, generator diameter 0.25 m ≤ D ≤ 4 m and Galileo number 2.105 ≤ ≤ 4.107. This revealed that the critical regime can develop smooth, rough and transition domains. Critical flow is very unstable; therefore, it is to be avoided in practice, which means that the dimensions and the slope of the conduit must be such that they do not cause such a state of flow. This explains why knowledge of the characteristics of the critical flow, including the frequency of its occurrence for a given installation, is essential.
The Mechanism of Action of Lactoferrin - Nucleoside Diphosphate Kinase
Complex in Combating Biofilm Formation
Authors: Sikarwar Juhi , Singh Jiya , Singh Tej P. , Sharma Pradeep , Sharma Sujata 
Published: August 2022

Background:
The ESKAPE group of pathogens which comprise of multidrug resistant
bacteria, namely Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae,
Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species are the cause of
deadly nosocomial infections all over the world. While these pathogens have developed robust
strategies to resist most antibiotics, their ability to form biofilms is one of their most combative
properties. Hence there is an urgent need to discover new antibacterial agents which could prevent
or destroy the biofilms made by these bacteria. Though it has been established that lactoferrin (LF),
a potent iron binding antibacterial, antifungal, and antiviral protein displays anti-biofilm properties,
its mechanisms of action, in addition to its iron chelation property, still remains unclear.

Objective:
The binding and inhibition studies of LF with the enzyme Nucleoside diphosphate
Kinase (NDK) and its elastase cleaved truncated 12 kDa fragment (12-NDK).

Methods:
The characterization studies of NDK and 12-NDK using florescence spectroscopy,
dynamic light scattering, size exclusion chromatography and ADP-glo Kinase Assay. Inhibition
studies of LF-NDK using ADP-glo kinase assay, Surface Plasmon Resonance and Biofilm
inhibition studies.

Results:
NDK and 12-NDK were cloned, expressed and purified from Acinetobacter baumannii and
Pseudomonas aeruginosa. The characterization studies revealed NDK and 12-NDK from both
species are stable and functional. The inhibition studies of LF-NDK revealed stable binding and
inhibition of kinase activity by LF.

Conclusion:
The binding and inhibition studies have shown that while LF binds with both the NDK
and their truncated forms, it tends to have a higher binding affinity with the truncated 12 kDa
fragments, resulting in their decreased kinase activity. This study essentially gives a new direction
to the field of inhibition of biofilm formation, as it proves that LF has a novel mechanism of action
in other than iron sequestration.
Chlorogenic Acid: A Dietary Phenolic Acid with Promising Pharmacotherapeutic
Potential
Authors: Singh Amit Kumar , Singla Rajeev Kumar , Pandey Abhay Kumar 
Published: August 2022

Abstract:
Phenolic acids are now receiving a great deal of interest as pervasive human dietary
constituents that have various therapeutic applications against chronic and age-related
diseases. One such phenolic acid that is being utilized in traditional medicine is chlorogenic
acid (CGA). It is one of the most readily available phytochemicals that can be isolated
from the leaves and fruits of plants, such as coffee beans (Coffea arabica L.), apples
(Malus spp.), artichoke (Cynara cardunculus L.), carrots (Daucus carota L.), betel
(Piper betle L.), burdock (Arctium spp.), etc. Despite its low oral bioavailability (about
33%), CGA has drawn considerable attention due to its wide range of biological activities
and numerous molecular targets. Several studies have reported that the antioxidant
and anti-inflammatory potentials of CGA mainly account for its broad-spectrum pharmacological
attributes. CGA has been implicated in exerting a beneficial role against dysbiosis
by encouraging the growth of beneficial GUT microbes. At the biochemical level, its
therapeutic action is mediated by free radical scavenging efficacy, modulation of glucose
and lipid metabolism, down-regulation of pro-inflammatory cytokines, such as tumor necrosis
factor-alpha (TNF-α), interleukin-6 (IL-6), IL-1β, and interferon-gamma (IFN-γ),
upregulation of nuclear factor erythroid 2-related factor 2 (Nrf-2), and inhibition of the
activity of nuclear factor- κβ (NF-κβ), thus helping in the management of diabetes, cardiovascular
diseases, neurodegenerative disorders, cancer, hypertension etc. This review
highlights the natural sources of CGA, its bioavailability, metabolism, pharmacotherapeutic
potential, and underlying mechanisms of action for the clinical usefulness of CGA in
the management of health disorders.
A Review on the Current Status of Homeopathy in the Clinical Manage-ment of Cancer
Authors: Ghosh Dipanjan , Tangelloju Ajay , Chakravarti Rudra , Singh Rajveer , Bhattacharya Bireswar , Ghosh Arijit , Bhutia Sujit Kumar , Ravichandiran Velayutham 
Published: August 2022

Abstract:
Homeopathy is a widely practiced alternate system of medicine around the world that employs small doses of various medicines to promote auto-regulation and self-healing. It is among the most commonly used alternative approaches in cancer and other diseases and alternative thera-peutic systems. It is widely used as palliative and as supportive therapy in cancer patients. Few cas-es have been reported on patients using homeopathy after surgery, radiotherapy, and chemotherapy, generally for overcoming side effects. The dose of Homoeopathic medicines and their mechanism of action in cancer has also been documented, while clinical trials on the effects of Homoeopathy in cancer treatment are rare. It is found that the anticancer potential of homeopathic medicines is re-ported for different cancer types, which show their efficacy through apoptosis and immune system modulation. Homeopathic treatment is an add-on to conventional therapy, with almost no interac-tion with the conventional drugs due to the small dose, and is largely attributed to improving lives by providing symptomatic relief, increasing survival time and boosting patient immunity. This re-view explores the accountability of the homeopathic system of medicine by highlighting some of the most commonly used homeopathic drugs for different types of cancers.
Iridoids as a Potential Hepatoprotective Class: A Review
Authors: de Carvalho Meirelles Gabriela , Bridi Raquel , von Poser Gilsane Lino 
Published: August 2022

Background:
Liver disease rates are gradually increasing over the years, becoming a severe
public health problem. The indiscriminate use of drugs associated with a rich fat diet, high consumption
of alcoholic beverages, and exposure to viral infections and lipid peroxidative products are
considered the chief factors for developing hepatic disorders. Owing to the absence of reliable hepatoprotective
drugs in the therapeutic arsenal, since they present a high incidence of adverse reactions
and/or lack of efficacy in some cases, liver diseases are widely treated with medicinal plants.
Among them are the plants producing iridoids, which are believed to be good remedies for liver disease
due to their bitter taste. The hepatoprotective effect of iridoids and extracts, rich in these compounds,
has been demonstrated, both in vitro and in vivo.

Objective:
This review aims to scrutinize the available literature related to the hepatoprotective activity
of iridoids.

Methods:
The information was obtained from scientific databases (Science Direct, PubMed, Web of
Science, Scopus, ACS Publications, Wiley Online Library) until December, 2021.

Results and Conclusion:
A total of 63 hepatoprotective iridoids were found, including aucubin, catalpol
and picroliv, a mixture of two iridoids. They are the target of a high number of studies, which
revealed their protective action against different hepatotoxic agents and detailed action mechanisms.

Results and Conclusion:
A total of 63 hepatoprotective iridoids were found, including aucubin, catalpol
and picroliv, a mixture of two iridoids. They are the target of a high number of studies, which
revealed their protective action against different hepatotoxic agents and detailed action mechanisms.
Psoralidin Induced Differentiation from Adipose-derived Stem Cells
to Nucleus Pulposus-like Cells by TGF-β/Smad Signaling
Authors: Li Shuofu , Liu Xiaorong , Nie Ying , Yang Lei , Zhang Chao , Guo Yantao , Yang Shaofeng , Li Zhaoyong 
Published: August 2022

Background:
Psoralidin (PL) could affect the differentiation of bone marrow
mesenchymal stem cells (BMSCs). The role of PL is still unclear in adipose-derived
stem cells (ADSCs).

Aims:
This study aimed to investigate the effects of PL on ADSCs differentiation into
nucleus pulposus-like cells and the TGF-β/Smad signaling pathway.

Methods:
The proliferation and apoptosis of ADSCs were detected. The nucleus
pulposus cell-related markers (CD24, BASP1, KRT19, and Aggrecan) and TGF-β/Smad
signaling pathway indexes were analyzed.

Results:
The results showed that compared to the control group, the cell activity was
increased in the PL group, and the apoptosis rate was decreased. The mRNA and
protein levels of nucleus pulposus cells markers (CD24, BASP1, KRT19, Aggrecan, and
Collagen Type II) and TGF-β/Smad signaling pathway-related indexes (TGF-β, SMAD2,
and SMAD3) were increased in PL group. After treatment with PL and TGF-β silencing,
the TGF-β/Smad signaling pathway-related indicators (TGF-β, SMAD2, and SMAD3)
and nucleus pulposus cells markers (CD24, BASP1, KRT19, Aggrecan, and Collagen
Type II) were found to be higher in the sh-TGF-β +PL group than in the sh-TGF-β group.

Conclusion:
In conclusion, our study showed that PL might induce the differentiation of
ADSCs to nucleus pulposus cells through the TGF-β/Smad signaling pathway. It might
have the potential application value in the treatment of intervertebral disc degeneration.
Synthesis and Characterization of New Bistriazole Quinazolinediones and
their Urease Inhibition Activities
Authors: Akyüz Gülay , Menteşe Emre 
Published: August 2022

Abstract:
A new series of bisthiosemicarbazide and bistriazole molecules bridged quinazolinedione
was synthesized and characterized by FT-IR, 1
H and 13C-NMR spectral data. Antiurease activities
of all new compounds were tested according to the phenol-hypochlorite method by Weatherburn. All
compounds have effective urease inhibition activities compared to the standard inhibitor thiourea and
previously synthesized quinazolinones as potential inhibitors. Bistriazoles containing quinazolinedione
have the best inhibition results. 1,3-Bis[(4-ethyl-5-mercapto-4H-1,2,4-triazol-3-yl)methyl]quinazoline2,4(1H,3H)-dione (5b) has the best inhibitory activity with 1.25 ± 0.45 µg/mL IC50 value.
Synthesis and Biological Evaluation of 4-substituted Aryl-piperazine Derivatives
of 1,7,8,9-tetrachloro-10,10-dimethoxy-4-azatricyclo [5.2.1.02,6]
dec-8-ene-3,5-dione as Anti-cancer and Anti-angiogenesis Agents
Authors: Guo Lifang , Xu Benshan , Hu Bin , Liu He , Song Nan 
Published: August 2022

Background:
A series of aryl-piperazine derivatives of 1,7,8,9-tetrachloro-10,10-dimethoxy-4-
azatricyclo [5.2.1.02,6] dec-8-ene-3,5-dione were designed, synthesized, and their biological activities
were evaluated.

Methods:
The chemical structures of the desired compounds were identified by 1H NMR, ESI-MS and
elementary analysis. The anti-cancer and anti-angiogenesis activities of the newly synthesized compounds
were evaluated by proliferation and migration assays, respectively.

Results:
The screening results demonstrated that compounds 2 and 5 showed potent anti-tumor activity
(IC50 values ranging from 7.1 to 15.9μM) with low cytotoxic activities (IC50＞79.3μM). Although compound
5 had no effects on endothelial proliferation (IC50=65.3μM), it significantly abrogated endothelial
cell migration (IC50=6.7μM).

Conclusion:
This work may impart new direction for the investigations of aryl-piperazine derivatives and
lead to the development of potent novel anti-tumor and anti-angiogenesis agents.
Unravelling the Mechanistic Role of ACE2 and TMPRSS2 in Hypertension: A Risk Factor for COVID-19
Authors: Govender Nalini , Khaliq Olive , Moodley Jagidesa , Naicker Thajasvarie 
Published: August 2022

Background:This review explores the mechanistic action of angiotensin-converting enzyme-2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) in the renin-angiotensin-aldosterone system (RAAS) that predisposes hypertensive patients to adverse outcome of severe COVID-19.Methods and Results:Entry of SARS-CoV-2 into the host cell via ACE2 disrupts the RAAS system, creating an imbalance between ACE and ACE2, and this together with an increased inflammatory response leads to hypertension (HTN), pulmonary vasoconstriction and acute respiratory distress. SARS-CoV-2 may also predispose infected individuals with existing HTN to a greater risk of severe COVID-19 complications. In the duality of COVID-19 and HTN, the imbalance of ACE and ACE2 results in an elevation of AngII and a decrease in Ang (1-7), a hyperinflammatory response and endothelial dysfunction. Endothelial dysfunction is the main factor that predisposes hypertensive patients to severe COVID-19 and vice-versa.Conclusion:Conclusion: Despite the increase in ACE2 expression in hypertensive SARS-CoV-2 infected pa-tients, ARBs/ACE inhibitors do not influence their severity and clinical outcomes, implicating continued usage. Future large scale clinical trials are warranted to further elucidate the association between HTN and SARS-CoV-2 infection; as well as the use of ARBs/ACEIs in SARS-CoV-2 hy-pertensive patients.
Possible Long-Term Cardiovascular Effects of COVID-19
Authors: Lugo Gloria Anahi , Nizami Hamasah , Haniff Fiyad , Su Lilly , Marsh Denise , Gupta Shefali , Jain Rohit , Goel Heenam 
Published: August 2022

Abstract:
Coronavirus Disease 2019 is caused by the severe acute respiratory syndrome coronavirus
2 (SARS-CoV-2) and has become a worldwide pandemic. Since 2019, the virus has mutated
into multiple variants that have made it harder to eradicate and have increased the rate of infection.
This virus can affect the structure and the function of the heart and can lead to cardiovascular
symptoms that can have long-lasting effects despite recovery from COVID-19. These symptoms
include chest pain, palpitations, fatigue, shortness of breath, rapid heartbeat, arrhythmias, cough
and hypotension. These symptoms may persist due to myocardial injury, cardiac inflammation or
systemic damage that may have been caused during infection. If these symptoms persist, the patient
should visit their cardiologist for diagnosis and treatment plan for any type of cardiovascular
disease that may have developed Post-COVID 19.