bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors associated with good quality of life among chronic objective pulmonary disease patients in Zhejiang province, China: a cross-sectional study
Authors: Ying Yubing , Khunthason Siriyaporn , Nilvarangkul Kessarawan 
Published: September 2022

Abstract
Background: Chronic obstructive pulmonary disease (COPD) is a chronic, progressive and debilitating disease that affects an individual's quality of life (QOL), especially those patients living in poor living environments. This study aimed to assess the level of good QOL and determine the factors associated with good QOL among COPD patients living in Zhejiang Province, China.
Methods: An analytical cross-sectional study was conducted to collect information about COPD patients in six tertiary hospitals in Zhejiang province. A validated questionnaire was used to collect general information, environmental factors, COPD stage, and treatment for the included patients. The standardized St. George's Respiratory Questionnaire (SGRQ) was used to assess QOL. Random sampling was used to recruit 420 participants. Questionnaires were completed by interviews. Binary logistic regression was used to predict influencing factors of good QOL among COPD patients living in Zhejiang Province, China at a significance level of α = 0.05.
Results: The overall good QOL among COPD patients was 25.7%. In the multivariable logistic regression, six variables were found to be associated with good QOL; airflow limitation severity (adjusted odds ratio (AOR)=8.69, 95% CI=2.65-28.46), duration of illness (AOR=8.25, 95% CI=1.98-34.29), number of hospitalisations within the past 3 months (AOR 5.39, 95% CI=1.07-27.24), annual treatment expenses (AOR=3.68, 95% CI=1.31-10.34), educational level (AOR=3.20, 95% CI=1.11-9.22), and type of medical insurance (AOR=2.21, 95% CI=1.04-4.71).
Conclusions: It is evident from this study that good QOL among COPD patients is strongly related to the stage of COPD and the financial burden on treatment. Early detection and treatment including exacerbations strategies are crucial to improving COPD patients’ QOL.
Development and Validation of an Individualized Immune Prognostic Signature in HNSCC
Authors: Yu Jintao , Sun Xia , Zhao Yichen , Zheng Chang , Zhou Baosen 
Published: September 2022

Abstract
Head and neck cancer is the seventh most common type of cancer worldwide, and the development of immunotherapy is conducive to the preservation of function and the improvement of prognosis. Based on immune gene database ImmPort and InnateDB, we investigated prognostic value of 546 head and necks squamous cell carcinoma samples from The Cancer Genome Atlas. We intersected of the two databases, selected specific module by WGCNA, and then performed univariate and multivariate Cox regression analyses to screen their prognostic roles in HNSCC patients. Three prognostic immune genes were identified as prognostic gene, including DEFB1, PTX3and SEMA3G. According to the risk scores of patients, we divided HNSCC patients into low- and high-risk subgroups by the median value of risk scores in each dataset. In addition, we calculated immune cells and immune infiltration of the two groups and constructed K-M survival curve plot based on immune infiltration result. Enrichment analysis indicated that cytokines and cytokine receptors interaction was more common in the specific immune gene module. Moreover, we constructed a nomogram based on the results of multivariate cox analysis and clinical information and confirmed the survival of the two groups by using two GEO database. We also established a correlation analysis between immune prognostic signature genes and immune checkpoints. Finally, we believed that PTX3 was the key gene, and the expression levels of PTX3 in 30 clinical samples was detected by immunohistochemical method. In conclusion, PTX3 is a key immune gene, which may contribute to poor prognosis of HNSCC by affecting TME.
Petrography and Stable Isotope Geochemistry of Middle Eocene Garampani Limestone (Assam), India: Implications for the Depositional Environment and Diagenesis
Authors: Singh Y. Raghumani , Devi Kh. Radhapiyari , Abbott Mark B. , Singh T. Lucky , Tongbram Guneshwar , Singh Ninthoujam Surdas 
Published: September 2022

Abstract

Petrography and stable isotopic (carbon and oxygen) studies of the Middle Eocene of Garampani limestone from Jamunagar limestone quarry of the Umrangso area, North Cachar Assam, were carried out to determine the environment of deposition and the level of diagenesis to improve our understanding of the climate at the time of formation. The major petrographic types documented here are include wackestone, packstone and grainstone which fall into the type I and II categories of the triangular classification scheme indicating formation in a short-lived, high-energy environment that was dissipating at the time of deposition. The samples have a distinct signature that includes extensive diagenesis with varying intensities of compaction, recrystallization, pressure solution, and neomorphism. Most of the samples produced positive δ13C ‰ ranges from 1.64 to 0.76‰ (PDB) and correlated with a transgressive phase. The negativeδ18Ovalues range from − 6.25 to -4.67‰ (PDB) suggesting that the Middle Eocene was generally characterized by a warmer climate.
The relation between the serum trough concentration of paracetamol and pain reduction in preterm and term neonates: a retrospective observational study
Authors: Berg Roland Benjamin van den , Laarman A. R. Céleste , Dijkstra Jacob A. , Veldkamp Agnes I. , Allegaert Karel , Swart Eleonora L. , Weissenbruch Mirjam M. van 
Published: September 2022

Abstract
Background Measuring concentrations of paracetamol could be a strategy to better understand the concentration-response profile for pain treatment in preterm and term neonates. It is not known if the serum trough concentration of paracetamol at steady state conditions is associated with a decrease in pain in this population. Low trough concentration could result in inadequate pain relief, like end-of-dose pain. Aim The aim of this study was to determine the association between the serum trough concentration of paracetamol and pain reduction in preterm and term neonates.
Method In this retrospective observational study a hospital database was used to select neonates who were treated with paracetamol intravenously or rectally for at least 48 hours. Linear regression was performed to determine if serum trough concentration of paracetamol at steady state conditions was a predictor for pain reduction. Pain reduction was defined as the difference between COMFORTneo scores before start and after the fifth dose of paracetamol.
Results 21 neonates were included for determining the association between serum trough concentration paracetamol and pain reduction. The median (IQR) of serum trough concentration of paracetamol after the fifth dose was 4.5 mg/L (2.7–8.5 mg/L). At steady state conditions the serum trough concentration of paracetamol was not a significant predictor of pain reduction in preterm and term neonates (p = 0.79 for preterm neonates and p = 0.49 for term neonates).
Conclusion No association was found between the serum trough concentration of paracetamol at steady state conditions and pain reduction in preterm and term neonates.
Fabrication of Chitosan Monolith by Thermally Induced Phase Separation of Chemically Modified Chitin to Remove Cu(II) Ions from the Water Solutions
Authors: Hajili Emil , Sugawara Akihide , Asoh Taka-Aki , Uyama Hiroshi 
Published: September 2022

Abstract
Chitosan (CS), an amino-polysaccharide, has applications in various areas, particularly in drinking water and wastewater treatment, with highly efficient properties for binding metal ions. Monoliths have been developed continuously for several decades, and today they hold an impressively strong position in highly efficient separation, ion exchange, catalysis, and chromatography. In our previous study, hierarchical chitin (CT) monolith was fabricated using chemically modified CT through the thermally induced phase separation (TIPS) method. This report generated highly effective, stable, and reusable CS monoliths by deacetylation of CT monoliths. The acquired monoliths demonstrated efficient removal of metal ions from the aqueous solution in a flow system. In order to gain durability in acidic and basic environments, epichlorohydrin (ECH) was used as a crosslinking agent. The crosslinked monoliths exhibited excellent performance in the adsorption of Cu(II) ions from the solution, and good reusability in multiple adsorption-desorption cycles without losing significant performance. The fabricated CS monolith can be modified and applied to various fields such as protein separation, catalyst, and drug delivery.
The Role of Body Appreciation in the Decision to Complete Metabolic and Bariatric Surgery Among Ethnically Diverse Patients
Authors: Kapera Olivia , Xie Luyu , Marroquín Elisa M. , Ngenge Sophia , Mathew M. Sunil , Almandoz Jaime P , Schellinger Jeffrey , Kukreja Sachin S. , Schneider Benjamin , McAdams Carrie , Messiah Sarah E. 
Published: September 2022

Abstract
Purpose. Metabolic and bariatric surgery (MBS) is an evidence-based safe, effective treatment for obesity. However, only half of referred or eligible persons complete the procedure for unknown reasons. The proposed study examined the association between the degree of body appreciation and the decision to complete MBS by ethnicity.
Methods. This prospective cohort study included 409 participants who had been referred to a bariatric surgeon or an obesity medicine program between August 2019 to May 2022. Participants completed a survey about health behaviors and psychosocial characteristics, including body appreciation by MBS completion status (Y/N). Crude and adjusted odd ratios of predictors of MBS completion were compared by ethnicity using logistic regression.
Results. The sample mean age was 47.18 years (SD 11.63), 86% were female. 40% identified as non-Hispanic White (NHW), 37.81% as non-Hispanic Black (NHB), and 18.08% as Hispanic. Over a third of the sample (31.2%, n=127) completed MBS. Overall, body appreciation did not differ between MBS completers and non-completers (p-value=0.70). Hispanic and NHB participants had greater body appreciation compared to NHWs (OR: 1.33, 95% CI: 1.16-1.51, p-value <.001; OR: 1.53, 95% CI: 1.27-1.86, p-value <.001, respectively). Multivariable logistic regression models revealed no significant association between body appreciation and MBS completion.
Conclusion. Although NHB and Hispanic MBS candidates had greater body appreciation than NHWs, body appreciation did not correlate with MBS completion.
Level of evidence
Level III: Evidence obtained from cohort or case-control analytic studies.
'I had nothing…and drugs don’t leave': A qualitative study exploring the drug use trajectories of young people who offend
Authors: Deans Emily Grace , Abdo Joseph , Ravulo Jioji , Conroy Elizabeth 
Published: September 2022

Abstract
Background
Alcohol and other drug use is heavily implicated in youth offending, with a significant proportion of young people who offend meeting the criteria for dependence and problematic substance use. Understanding the drivers of drug use behaviours among this sample has important implications for recognising the needs of young people and preventing recidivism.
Methods
Qualitative inquiry through semi-structured focus group discussions (n = 9, 30 participants) was employed to privilege the young people’s perspectives. Discussions were audio recorded and a constant comparative approach to data analysis was used to identify similarities and differences in participants accounts.
Results
Findings were consistent with Agnew’s [1992] General Strain Theory (GST), as many participants had experienced significant disadvantage which influenced their use of illicit substances. Participants’ experience of AOD use as a means of mitigating negative emotion was cascaded across the loss of something of value, unjust treatment, and the inability to achieve ‘success’ in life.
Conclusions
Our understanding of the way in which strain can drive drug use behaviours provides insight into strategies to support young people in contact with the justice system. We must work hard to see a more connected system, to advocate for more helpful discourses around youth offending, and to see the equitable distribution of health education and early intervention for all young people.
Structural, Magnetic and Thermal Characteristic Analysis of Synthesized Magnetite Spinel Ferrite Nanoparticles
Authors: Gogoi Bandana , Das Upamanyu 
Published: September 2022

Abstract
Spinel ferrite nanoparticles are potential candidates for multiple biomedical applications. Spinel ferrite nanoparticles have been studied extensively for understanding physical, chemical, electro-optical as well as magnetic properties which are fascinating due to cationic distributions corresponding to tetrahedral sites and octahedral sites in a cubic phase. Biocompatibility and large magnetic moment are basic requirements in spinel ferrite nanoparticles for efficient functioning in specific application purpose. Fe3O4 (magnetite) is an important member of spinel ferrite group with high chemical stability and ferrimagetic material property at nanodimension. Superparamagnetic state and biocompatibility of magnetite (Fe3O4) spinel ferrite nanoparticle has already been proven. Spinel ferrite magnetite nanoparticles have been developed based on precipitation of iron oxide using ferric and ferrous ions at the ratio 2:1 in alkaline media at and above 1000C. The experimental parameters have been set to synthesize pure and uniformly sized magnetite nanoparticles. No other phases of iron oxides were detected other than magnetite spinel phase in the XRD result. The average crystal size has been determined from XRD peak broadening. Absorption spectra were investigated using UV-Vis Spectrometer and FTIR. Thermal and magnetic measurements were also been carried out. One sample of the prepared nanoparticles with polymer coating of polyvinyl alcohol has been studied for superparamagnetic nature. Superparamagnetic particles show saturation value of magnetization 51.26 emu/g at 100 K. ZFC-FC curves for two samples with polymer coating of polyvinyl alcohol and hydroxy-propyl methyl cellulose have also been studied.
Regional Dialect Diversity and Corporate Cash Holdings: Evidence from China
Authors: Guo Lian , Chen Diqiang 
Published: September 2022

Abstract

Using the sample of listed private firms in China from 1999 to 2019, We examine the relation between the regional dialect diversity and corporate cash holdings. The main finding reveals that firms in higher dialect diversity city hoard more cashes. More importantly, further analyses indicate that the positive relation between dialect diversity and cash holdings are more pronounced among financing constrained firms. Overall, we find evidence in favor of precautionary savings motive predicts that firms located in city with higher dialect diversity will hoard more cash to compensate for reduced access to capital markets.
Discrepant effects of flooding on assembly processes of abundant and rare communities in riparian soils
Authors: Ye Fei , Sun Zhaohong , Moore Selina Sterup , Wu Jiapeng , Hong Yiguo , Wang Yu 
Published: September 2022

Abstract
Numerous rare species coexist with a few abundant species in microbial communities, and together play an essential role in riparian ecosystems. Relatively little is understood, however, about the nature of assembly processes of these communities and how they respond to a fluctuating environment. In this study, drivers controlling the assembly of abundant and rare subcommunities for bacteria and archaea in a riparian zone were determined, and their resulting patterns on these processes analyzed. Abundant and rare bacteria and archaea showed a consistent variation on the community structures along the riparian elevation gradient, which was closely associated with flooding frequency. The community assembly of abundant bacteria was not affected by any measured environmental variables, while soil moisture and amount of time submerged were the two most decisive factors determining rare bacterial community. Assembly of abundant archaeal community was also determined by these two factors, whereas rare archaea was significantly associated with soil carbon-nitrogen ratio and total carbon content. The assembly processes of abundant and rare bacterial subcommunity were driven respectively by dispersal limitation and variable selection. Undominated processes and dispersal limitation dominated the assembly of abundant archaea, whereas homogeneous selection primarily driven rare archaea. Flooding may therefore play a crucial role in determining the community assembly processes by imposing disturbances and shaping soil niches. Overall, this study reveals the assembly patterns of abundant and rare communities in the riparian zone and provides further insight into the importance of their respective roles in maintaining a stable ecosystem during times of environmental perturbations.
Multi-proteomic analysis of 5xFAD mice reveals new molecular signatures for early-stage Alzheimer’s disease
Authors: Jo Yeon Suk , Park Geuna , Jang Kuk-In , Bae Sungwon , Kwon Yang Woo , Lee Seulah , Jang Jin-Hyeok , Lee Chany , Oh Yong-Seok , Yoon Jong Hyuk 
Published: September 2022

Abstract
Background
Alzheimer’s disease (AD) early diagnosis remains difficult due to limitations in clinical exams and amyloid plaque imaging.
Methods
In the present study, the hippocampus, cortex, and blood plasma extracellular vesicles (EVs) from 3- and 6-month-old 5xFAD mice were analysed by reliable quantitative proteomics approach.
Results
The 3- and 6-month-old hippocampus and cortex proteome in both the age groups showed similar features in functional annotation and canonical pathway analysis, but the significantly changed proteins were rarely overlapped. Furthermore, the plasma EVs proteome showed significantly different informatic features compared with other proteomes. Depending on the AD stage, proteomic profiles undergo drastic changes in brain subregion- and in tissue-specific manners. Notably, regulations of several canonical pathways, including PI3K/Akt signalling, were differing between the hippocampus and cortex. Furthermore, we identified eight potential biomarkers that can detect early-stage AD (integrin alpha-IIb (ITGA2B), sulfhydryl oxidase 1, phospholipid transfer protein, talin (TLN), heat shock 70 kDa protein 1 (HSPA1L), alpha-2 macroglobulin (A2M), platelet factor 4, and filamin A (FLNA)) and validated them, using plasma EVs of stage-divided patients with AD.
Conclusion
ITGA2B, TLN, HSPA1L, and A2M were finally selected by machine learning modelling as distinguishing biomarkers for normal and early-stage AD with 85% accuracy. The present study provides insights into AD pathogenesis and identifies novel early-stage AD biomarkers.
Binding behavior and in vitro cytotoxicity of inclusion complexes between aminopterin and cucurbit[7]uril
Authors: Chang Juan , Wang Ke , Chen Jue , Chang Yinxia X. 
Published: September 2022

Abstract
Aminopterin (AMT) is a kind of universal antineoplastic drugs, but it has severe toxic and side effects, leaving it rarely used in clinic. Herein, we found that cucurbit[7]uril (CB[7]) and AMT can form stable inclusion complexes, and the formation of CB[7]-AMT 2:1 supramolecular inclusion complex was confirmed by UV-visible absorption spectra, fluorescence spectra, and molecular modeling calculations in aqueous solution. Binding stability constants (Ks) were determined by UV-visible and fluorescence spectra method, with 1.97 × 105L·mol–1and 2.29 × 105 L·mol–1, respectively. The binding energy was calculated to be 28.8 kcal·mol–1 for the CB[7]-AMT complex. And then, through a series of cell experiments of CCK8 assay, DAPI staining and hoechst33342/PI double staining, we fully proved that the CB[7]-AMT complex can reduce the toxicity of AMT to normal cells such as hepatocyte line LO2, and improve its anticancer effect on cancer cells overexpressing spermine, typically like human colon cancer cell line HCT116. It confirmed that the CB[7]-AMT complex had the effect of reducing toxicity and increasing efficiency. These results indicated that CB[7]-AMTinclusion complex might be a promising novel formulation of AMT for its clinical development.
Research on Multi-Dimensional Pricing Model of UBI Auto Insurance Based on Fuzzy Controller
Authors: Wang Xindong , Yan Chun , Liu Wei , Liu Xinhong 
Published: September 2022

Abstract
Aiming at the problem of usage-based insurance (UBI) auto insurance pricing, this paper proposes a multi-dimensional pricing model based on fuzzy controllers. In order to comprehensively consider the influencing factors of the rate, an index system of autonomous underwriting coefficient is proposed from four dimensions of driving distance, driving time, driving speed and driving behavior. A sub-fuzzy controller based on dimension index is proposed. The adaptive weight and learning factor are introduced into simulated annealing particle swarm optimization (SAPSO) algorithm, and combined with Metropolis criterion, the adaptive SAPSO algorithm is improved. In order to reduce the subjective defects of fuzzy controller, standard particle swarm optimization (PSO), parameter adaptive PSO and SAPSO algorithms are used for optimization respectively. The fitness function is improved to incorporate stability into the screening criteria. After comparison, it is found that SAPSO algorithm can get the optimal results in fuzzy controllers with lower dimensions, and parameter adaptive PSO algorithm in fuzzy controllers with higher dimensions can get better results. The optimal output result is selected and input into the total fuzzy controller to calculate the autonomous underwriting coefficient. The experimental results show that the multi-dimensional auto insurance pricing model based on fuzzy controller in this paper can better measure the driving risk comprehensively. It has higher accuracy and stability in rate determination.
CRISPR/Cas9 and FLP-FRT mediated multi-modular engineering of the cis-regulatory landscape of the bithorax complex of Drosophila melanogaster
Authors: Hajirnis Nikhil , Pandey Shubhanshu , Mishra Rakesh 
Published: September 2022

Abstract

The Homeotic genes or Hox define the anterior-posterior (AP) body axis formation in bilaterians and are often present on the chromosome in an order which is collinear to their function across the AP axis. However, there are many cases wherein the Hox are not collinear, but their expression pattern is conserved across the AP axis. The expression pattern of Hoxis attributed to the cis-regulatory modules (CRMs) consisting of enhancers, initiators, or repressor elements that together regulate the genes in a segment-specific manner. In the Drosophila melanogaster Hoxcomplex, the bithorax complex (BX-C), even the CRMs are organized in an order that is collinear to their function in the thoracic and abdominal segments. In the present study, we performed in-silico analysis of the available ChIP data followed by systematic curation of experimentally validated regions of the BX-C gene, Abd-B, to generate functionally relevant map of the regulatory landscape. Next, the regulatorily inert regions were targeted using CRISPR/Cas9 to generate a series of transgenic lines with the insertion of FRT sequences. Further, these FRT lines are repurposed to shuffle the CRMs associated with Abd-B to generate modular deletion, duplication, or inversion of multiple CRMs. The rearrangements yielded entirely novel phenotypes in the fly suggesting the requirement of such complex manipulations to address the significance of higher order arrangement of the CRMs. The functional map and the transgenic flies generated in this study are important resource to decipher the collective ability of multiple regulatory elements in eukaryotic genome to function as complex modules.