bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the accessibility of sport stadia for people with disability: towards the development of a Stadium Accessibility Scale (SAS)
Authors: Kitchin Paul J. , Paramio-Salcines Juan Luis , Darcy Simon , Walters Geoff 
Published: January 2022

PurposeThe aim of this paper is to synthesise existing literature on sports stadia, spectators with disabilities (SwD) and accessibility to identify themes and to highlight the gaps in the literature. This review subsequently develops two propositions that will enable research in this emerging area to further develop.Design/methodology/approachThe research was guided by two research questions: First, what does the peer reviewed evidence tell us about SwD and the accessibility of sporting stadia?; Second, how can this information be used to develop a Stadium Accessibility Scale (SAS)? The authors conducted a rapid review of the literature across three databases that identified 34 papers for synthesis.FindingsThe synthesis revealed three research themes: a focus on legislative compliance, the need to enhance resources (both physical and human) and research that focusses on moving beyond the stadium experience. The latter can be subdivided into two streams – studies that look at accessibility as a social legacy of major events and studies that seek to understand the whole journey that SwD's must make to attend sport events.Research limitations/implicationsThe study makes two key recommendations. The first is to encourage further research aligned to the HOPES framework (Paramio-Salcines et al., 2016) that explicitly recognises the importance of understanding the broader approach to the customer experience. The second is the need for the development and validation of a reliable SAS.Originality/valueGreater accessibility provides a foundation for inclusive environments in sport. The findings are relevant for all stakeholders in sport as universal accessibility benefits more than people with disabilities.
Sweep netting samples, but not sticky trap samples, indicate beneficial arthropod abundance is negatively associated with landscape wide insecticide use
Authors: Bakker Lieneke , van der Werf Wopke , Bianchi Felix J. J. A. 
Published: January 2022

Abstract

Insecticide use and landscape context are major drivers for the abundance of beneficial arthropods, such as predators, parasitoids and pollinators. However, the relative importance of local and landscape‐wide insecticide use is not well understood, and it is unclear to what extent impacts of insecticides on beneficial arthropod populations are moderated by landscape composition.

We collected and analysed data on beneficial arthropod abundance and local and landscape‐wide insecticide use across 38 Dutch landscapes. We used regression to study the associations between beneficial arthropod abundance (response), insecticide use and landscape factors.

Insects were sampled by sweep netting and yellow sticky traps in field margins (‘sampling sites’) in the summer of 2017 and 2018. We paired organic and conventional sites in the same landscape setting. We used three indicators of insecticide use: management (organic vs. conventional), Treatment Frequency Index of insecticides applied in the field adjacent to the sampling site, and the estimated landscape‐wide average quantity of insecticides applied per ha within a 1 km radius around the sampling site.

Abundances of beneficial arthropods in field margins, assessed by sweep netting, were negatively associated with the estimated quantity insecticide applied in the surrounding landscape, while arthropod sampling by sticky traps did not show this relationship. Pollinator abundance was negatively associated with conventional management and with the frequency of insecticide applications in the adjacent field, while predator and parasitoid abundance did not show significant associations with local insecticide use indicators. Negative impacts of insecticide use were not moderated in more diverse landscapes with higher proportions of semi‐natural habitats.

Policy implications. Insecticide impacts on arthropod populations extend beyond the fields in which they are used. Awareness of the potential landscape‐wide effects of pesticides, either through drift of pesticides or insect movement, may encourage land managers to adopt practices to reduce insecticide drift and the frequency of insecticide applications, thereby reducing the impacts of landscape‐wide exposure on arthropods that provide services to agriculture. Risk assessments of insecticides and pesticide use regulations need to account for potential spill‐over effects and consider landscape‐scale consequences associated with the source–sink dynamics of arthropods.
Isolation and characterization of a novel Lambda‐like phage infecting the bloom‐forming cyanobacteria Cylindrospermopsis raciborskii
Authors: Laloum Emmanuelle , Cattan‐Tsaushu Esther , Schwartz Daniel A. , Shaalan Hanaa , Enav Hagay , Kolan Dikla , Avrani Sarit 
Published: January 2022

SummaryCylindrospermopsis raciborskii is a central bloom‐forming cyanobacteria. However, despite its ecological significance, little is known of its interactions with the phages that infect it. Currently, only a single sequenced genome of a Cylindrospermopsis‐infecting phage is publicly available. Here we describe the isolation and characterization of Cr‐LKS3, a second phage infecting Cylindrospermopsis. Cr‐LKS3 is a siphovirus with a higher genome similarity to prophages within heterotrophic bacteria genomes than to any other cyanophage/cyano‐prophage, suggesting that it represents a novel cyanophage group. The function, order and orientation of the 72 genes in the Cr‐LKS3 genome are highly similar to those of Escherichia virus Lambda (hereafter Lambda), despite the very low sequence similarity between these phages, showing high evolutionary convergence despite the substantial difference in host characteristics. Similarly to Lambda, the genome of Cr‐LKS3 contains various genes that are known to be central to lysogeny, suggesting it can enter a lysogenic cycle. Cr‐LKS3 has a unique ability to infect a host with a dramatically different GC content, without carrying any tRNA genes to compensate for this difference. This ability, together with its potential lysogenic lifestyle shed light on the complex interactions between C. raciborskii and its phages.
Enzyme‐constrained models predict the dynamics of Saccharomyces cerevisiae growth in continuous, batch and fed‐batch bioreactors
Authors: Moreno‐Paz Sara , Schmitz Joep , Martins dos Santos Vitor A. P. , Suarez‐Diez Maria 
Published: January 2022

SummaryGenome‐scale, constraint‐based models (GEM) and their derivatives are commonly used to model and gain insights into microbial metabolism. Often, however, their accuracy and predictive power are limited and enable only approximate designs. To improve their usefulness for strain and bioprocess design, we studied here their capacity to accurately predict metabolic changes in response to operational conditions in a bioreactor, as well as intracellular, active reactions. We used flux balance analysis (FBA) and dynamic FBA (dFBA) to predict growth dynamics of the model organism Saccharomyces cerevisiae under different industrially relevant conditions. We compared simulations with the latest developed GEM for this organism (Yeast8) and its enzyme‐constrained version (ecYeast8) herein described with experimental data and found that ecYeast8 outperforms Yeast8 in all the simulations. EcYeast8 was able to predict well‐known traits of yeast metabolism including the onset of the Crabtree effect, the order of substrate consumption during mixed carbon cultivation and production of a target metabolite. We showed how the combination of ecGEM and dFBA links reactor operation and genetic modifications to flux predictions, enabling the prediction of yields and productivities of different strains and (dynamic) production processes. Additionally, we present flux sampling as a tool to analyse flux predictions of ecGEM, of major importance for strain design applications. We showed that constraining protein availability substantially improves accuracy of the description of the metabolic state of the cell under dynamic conditions. This therefore enables more realistic and faithful designs of industrially relevant cell‐based processes and, thus, the usefulness of such models.
Association between IGF‐1 levels ranges and all‐cause mortality: A meta‐analysis
Authors: Rahmani Jamal , Montesanto Alberto , Giovannucci Edward , Zand Hamid , Barati Meisam , Kopchick John J. , Mirisola Mario G. , Lagani Vincenzo , Bawadi Hiba , Vardavas Raffaele , Laviano Alessandro , Christensen Kaare , Passarino Giuseppe , Longo Valter D. 
Published: January 2022

AbstractThe association between IGF‐1 levels and mortality in humans is complex with low levels being associated with both low and high mortality. The present meta‐analysis investigates this complex relationship between IGF‐1 and all‐cause mortality in prospective cohort studies. A systematic literature search was conducted in PubMed/MEDLINE, Scopus, and Cochrane Library up to September 2019. Published studies were eligible for the meta‐analysis if they had a prospective cohort design, a hazard ratio (HR) and 95% confidence interval (CI) for two or more categories of IGF‐1 and were conducted among adults. A random‐effects model with a restricted maximum likelihood heterogeneity variance estimator was used to find combined HRs for all‐cause mortality. Nineteen studies involving 30,876 participants were included. Meta‐analysis of the 19 eligible studies showed that with respect to the low IGF‐1 category, higher IGF‐1 was not associated with increased risk of all‐cause mortality (HR = 0.84, 95% CI = 0.68–1.05). Dose–response analysis revealed a U‐shaped relation between IGF‐1 and mortality HR. Pooled results comparing low vs. middle IGF‐1 showed a significant increase of all‐cause mortality (HR = 1.33, 95% CI = 1.14–1.57), as well as comparing high vs. middle IGF‐1 categories (HR = 1.23, 95% CI = 1.06–1.44). Finally, we provide data on the association between IGF‐1 levels and the intake of proteins, carbohydrates, certain vitamins/minerals, and specific foods. Both high and low levels of IGF‐1 increase mortality risk, with a specific 120–160 ng/ml range being associated with the lowest mortality. These findings can explain the apparent controversy related to the association between IGF‐1 levels and mortality.
Accounting and First Nations: A Systematic Literature Review and Directions for Future Research
Authors: Norris Ellie , Kutubi Shawgat , Greenland Steven 
Published: January 2022

AbstractThis paper presents a synthesis of academic research focused on First Nations peoples, contrasting First Nations versus non‐Indigenous understandings of accounting and accountability. Key themes and trends in past research are identified across 51 publications spanning four decades, and directions for future research are proposed. The need for more culturally responsive accounting is well established, and past studies highlight the inadequacies of reporting practices which do not appear to capture the priorities and nuances of First Nations entities. The focus and execution of accounting research is shifting towards more contemporary experiences with accounting, and the contribution of First Nations worldviews to advances in non‐financial reporting. This paper systematically explains the inadequacies of contemporary reporting practices and encourages the accounting community to reflect on future opportunities. It is therefore relevant to both academics and practitioners seeking to uphold the rights of First Nations peoples to self‐determination in line with the United Nations Declaration on the Rights of Indigenous Peoples. Further work is urgently required to ensure First Nations organisations are adequately supported in their reporting practices, to incorporate traditional knowledges and to achieve positive outcomes for their communities.
Lost mutualisms: Seed dispersal by Sumatran rhinos, the world’s most threatened megafauna
Authors: McConkey Kim R. , Aldy Firmann , Ong Lisa , Sutisna Deden Jaya , Campos‐Arceiz Ahimsa 
Published: January 2022

AbstractDiverse assemblages of seed‐dispersing megafauna once existed in Asian rainforests, but are now almost solely represented by elephants. Asia's rhinos persist in remnant, ecologically extinct populations and the most threatened of these is the Sumatran rhino, Dicerorhinus sumatrensis. To understand the seed dispersal role of Sumatran rhinos, we consolidated information on fruit consumption, seed dispersal, and fruit traits from a 2‐month field study (Sumatra), local ecological knowledge (Peninsular Malaysia), and published and unpublished accounts. We evaluated differences between the taxa and traits of fruits dispersed by rhinos and elephants, and identified other dispersers of megafaunal‐syndrome fruits that were rhino‐dispersed. At least 79 plant species were dispersed by rhinos: overstorey plants (trees and climbers; 78% of species) had large, usually “mammal‐colored,” fruits and seeds, and were mainly drupes and berries; 61% of these were megafaunal‐syndrome fruits (>4 cm wide). Understorey plants (herbs, shrubs, and small trees) had small, often capsular, fruits and seeds that are potentially dispersed following the “foliage‐is‐the‐fruit” hypothesis. Rhinos were the only known disperser for 35% of the megafaunal‐fruit genera. The highest dispersal overlap shown was with elephants: fruits dispersed by rhinos tended to be capsular and were smaller than fruits dispersed by both elephants and rhinos. Given these findings and the different foraging and ranging behavior of Sumatran rhinos and elephants, we suggest that these megafauna had important differences in their seed dispersal roles. Asian rainforests have, therefore, lost an important seed dispersal mutualist. Conservation efforts should aim to protect and restore the ecological function of these unique creatures.Abstract in Indonesian is available with online material.
Recruitment limitation in three large‐seeded plant species in a tropical moist forest
Authors: Garzon‐Lopez Carol X. , Barcenas Eduardo Medina , Ordoñez Alejandro , Jansen Patrick A. , Bohlman Stephanie A. , Olff Han 
Published: January 2022

AbstractRecruitment limitation—the failure of a species to establish recruits at an available site—is a potential determinant of plant communities’ structure, causing local communities to be a limited subset of the regional species pool. Recruitment limitation results from three mechanisms: (i) lack of seed sources (i.e., source limitation), (ii) failure of available seeds to reach recruitment sites (i.e., dispersal limitation), and (iii) failure of arrived seeds to establish at a location (i.e., establishment limitation). Here, we evaluated the relative importance of these mechanisms in three co‐occurring tree species (Dipteryx oleifera,Attalea butyracea, andAstrocaryum standleyanum) that share seed dispersers/predators. The study was set up on Barro Colorado Island (Panama) at 62 one‐ha sites with varying tree densities. Source limitation was estimated as the proportion of sites that would be reached by seeds if seeds were distributed uniformly. Dispersal limitation was estimated from the number of sites with seeds in the soil bank. Establishment limitation was evaluated by measuring germination and 1‐year survival in seed addition experiments. The effect of conspecific and heterospecific densities on the mechanisms was evaluated at three spatial scales (1, 5, and 9 ha). For all species, seed predation was the most important recruitment component (~80% decrease in seed survival). Establishment varied among species and was affected by conspecific and heterospecific species densities across spatial scales. Given that species identity, distribution, and seed dispersal/predation affect recruitment at multiple scales, multiscale studies are required to understand how recruitment limitation determines community structure in tropical forests.Abstract in Spanish is available with online material.
Exosomes from M1‐polarized macrophages promote apoptosis in lung adenocarcinoma via the miR‐181a‐5p/ETS1/STK16 axis
Authors: Wang Xuan , Huang Renhong , Lu Zhouyi , Wang Zheng , Chen Xiaofeng , Huang Dayu 
Published: January 2022

AbstractSerine/threonine kinase 16 (STK16) is crucial in on regulating tumor cell proliferation, apoptosis, and prognosis. Activated M1 macrophages regulate lung adenocarcinoma (LUAD) growth by releasing exosomes. This study aims to investigate the role of STK16 and then focus on the possible mechanisms through which exosomes derived from M1 macrophages play their roles in LUAD cells by targeting STK16. Clinical LUAD samples were used to evaluate the expression of STK16 and its association with prognosis. Exosomes were isolated from M0 and M1 macrophages by ultracentrifugation and were then identified by electron microscopy and western blotting. In vitro gain‐ and loss‐of‐function experiments with LUAD cells were performed to elucidate the functions of miR‐181a‐5p, ETS1, and STK16, and mouse xenograft models were used to verify the function of STK16 in vivo. Western blotting, quantitative real‐time PCR, CCK‐8 assay, cell apoptosis, immunohistochemistry staining, luciferase assay, ChIP assay, and bioinformatics analysis were performed to reveal the underlying mechanisms. High expression of STK16 was observed in LUAD tissues and cells, and higher expression of STK16 was associated with worse prognosis. Silencing STK16 expression inhibited cell proliferation and promoted apoptosis via the AKT1 pathway. Exosomes from M1 macrophages inhibited viability and promoted apoptosis by inhibiting STK16. Moreover, miR‐181a‐5p is the functional molecule in M1 macrophage‐derived exosomes and plays a vital role in inhibiting cell proliferation and promoting apoptosis by targeting ETS1 and STK16. Hence, exosomes derived from M1 macrophages were capable of inhibiting viability and promoting apoptosis in LUAD via the miR‐181a‐5p/ETS1/STK16 axis.
Contemporary selection on MHC genes in a free‐living ruminant population
Authors: Huang Wei , Dicks Kara L. , Hadfield Jarrod D. , Johnston Susan E. , Ballingall Keith T. , Pemberton Josephine M. 
Published: January 2022

AbstractGenes within the major histocompatibility complex (MHC) are the most variable identified in vertebrates. Pathogen‐mediated selection is believed to be the main force maintaining MHC diversity. However, relatively few studies have demonstrated contemporary selection on MHC genes. Here, we examine associations between MHC variation and several fitness measurements including total fitness and five fitness components, in 3400 wild Soay sheep (Ovis aries) monitored between 1989 and 2012. In terms of total fitness, measured as lifetime breeding success of all individuals born, we found haplotypes named C and D were associated with decreased and increased male total fitness respectively. In terms of fitness components, juvenile survival was associated with haplotype divergence while individual haplotypes (C, D and F) were associated with adult fitness components. Consistent with the increased male total fitness, the rarest haplotype D has increased in frequency throughout the study period more than expected under neutral expectations. Our results demonstrate that contemporary natural selection is acting on MHC class II genes in Soay sheep and that the mode of selection on specific fitness components can be different mode from selection on total fitness.
The perceptions of general practice among Central and Eastern Europeans in the United Kingdom: A systematic scoping review
Authors: Poppleton Aaron , Howells Kelly , Adeyemi Isabel , Chew‐Graham Carolyn , Dikomitis Lisa , Sanders Caroline 
Published: January 2022

AbstractBackgroundAround 2 million people have migrated from Central and Eastern Europe to the UK since 2004. The UK Central and Eastern European Community (UK‐CEE) are disproportionately exposed to the social determinants of poor physical and mental health. Their health and healthcare beliefs remain under‐researched, particularly regarding primary care.ObjectiveThis review explores UK‐CEE community members' use and perceptions of UK general practice.MethodsA systematic search of nine bibliographic databases identified 2094 publications that fulfilled the search criteria. Grey literature searches identified 16 additional relevant publications. Screening by title and abstract identified 201 publications of relevance, decreasing to 65 after full‐text screening. Publications were critically appraised, with data extracted and coded. Thematic analysis using constant comparison allowed generation of higher‐order thematic constructs.ResultsFull UK‐CEE national representation was achieved. Comparatively low levels of GP registration were described, with ability, desire and need to engage with GP services shaped by the interconnected nature of individual community members' cultural and sociodemographic factors. Difficulties overcoming access and in‐consultation barriers are common, with health expectations frequently unmet. Distrust and dissatisfaction with general practice often persist, promoting alternative health‐seeking approaches including transnational healthcare. Marginalized UK‐CEE community subgroups including Roma, trafficked and homeless individuals have particularly poor GP engagement and outcomes. Limited data on the impact of Brexit and COVID‐19 could be identified.ConclusionsReview findings demonstrate the need for codesigned approaches to remove barriers to engagement, culturally adapt and develop trust in GP care for UK‐CEE individuals.Community InvolvementCommunity members and stakeholders shaped the conceptualisation of the review question and validation of emergent themes.
Brunsting‐Perry pemphigoid: a systematic review
Authors: Aromolo Italo Francesco , Maronese Carlo Alberto , Moltrasio Chiara , Genovese Giovanni , Marzano Angelo Valerio 
Published: January 2022

AbstractBackgroundBrunsting‐Perry pemphigoid (BPP) is a rare, autoimmune bullous skin disorder classified within the spectrum of mucous membrane pemphigoid (MMP).Materials and MethodsAn a priori protocol was designed based on PRISMA guidelines. PubMed and Scopus databases were searched for English‐language articles concerning BPP published between 1950 and July 2021.ResultsThirty‐six articles including 63 BPP patients were analyzed. The mean age at diagnosis was 62.9 years (range: 27‐86). BPP was shown to be characterized by vesiculobullous lesions (46/63, 73.0%) on an erythematous base, erosions or ulcerations (27/63, 42.9%), atrophic scars (49/63, 77.8%), and milia (4/63, 6.3%). Exclusive oral mucosal involvement was documented in 22.2% of cases, usually manifesting after the cutaneous onset of the disease. Subepidermal blistering was a constant finding, often with an eosinophil‐rich inflammatory infiltrate (21/58, 36.2%). Positive direct immunofluorescence was found in 92.0% of patients, almost always with linear IgG ± C3 deposits along the basement membrane (43/46, 93.5%). BP180 (12/15, 80.0%), BP230 (5/15, 33.3%), and laminin 332 (3/15, 20.0%) were the most frequently identified target antigens.ConclusionsBPP nosologic position remains uncertain, given the overlap with other autoimmune bullous diseases, such as MMP, bullous pemphigoid, and epidermolysis bullosa acquisita, particularly in its BPP‐like variant. Nonpredominant oral mucosal lesions may appear during the course of the disease, generally after cutaneous manifestations. Positivity of DIF and anti‐BP180/230 autoantibodies detected on ELISA/immunoblotting in the absence of anticollagen VII antibodies may provide guidance in diagnosing BPP.