bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential interactions of resting, activated, and desensitized states of the α7 nicotinic acetylcholine receptor with lipidic modulators
Authors: Zhuang Yuxuan , Noviello Colleen M , Hibbs Ryan E , Howard Rebecca J , Lindahl Erik 
Published: April 2022

AbstractThe α7 nicotinic acetylcholine receptor is a pentameric ligand-gated ion channel that modulates neuronal excitability, largely by allowing Ca2+ permeation. Agonist binding promotes transition from a resting state to an activated state, and then rapidly to a desensitized state. Recently, cryo-EM structures of the human α7 receptor in nanodiscs were reported in multiple conformations. These were selectively stabilized by inhibitory, activating, or potentiating compounds. However, the functional annotation of these structures, and their differential interactions with unresolved lipids and ligands, remain incomplete. Here, we characterized their ion permeation, membrane interactions, and ligand binding using computational electrophysiology, free-energy calculations, and coarse-grained molecular dynamics. In contrast to non-conductive structures in apparent resting and desensitized states, the structure determined in the presence of the potentiator PNU-120596 was consistent with an activated state permeable to Ca2+. Transition to this state was associated with compression and rearrangement of the membrane, particularly in the vicinity of the peripheral MX helix. An intersubunit transmembrane site was implicated in selective binding of either PNU-120596 in the activated state, or cholesterol in the desensitized state. This substantiates functional assignment of all three lipid-embedded α7-receptor structures with ion permeation simulations. It also proposes testable models of their state-dependent interactions with lipophilic ligands, including a mechanism for allosteric modulation at the transmembrane subunit interface.
Modeling and mechanical perturbations reveal how spatially regulated anchorage gives rise to spatially distinct mechanics across the mammalian spindle
Authors: Suresh Pooja , Galstyan Vahe , Phillips Rob , Dumont Sophie 
Published: April 2022

ABSTRACTDuring cell division, the spindle generates force to move chromosomes. In mammals, microtubule bundles called kinetochore-fibers (k-fibers) attach to and segregate chromosomes. To do so, k-fibers must be robustly anchored to the dynamic spindle. We previously developed microneedle manipulation to mechanically challenge k-fiber anchorage, and observed spatially distinct response features revealing the presence of heterogeneous anchorage (Suresh et al. 2020). How anchorage is precisely spatially regulated, and what forces are necessary and sufficient to recapitulate the k-fiber’s response to force remain unclear. Here, we develop a coarse-grained k-fiber model and combine with manipulation experiments to infer underlying anchorage using shape analysis. By systematically testing different anchorage schemes, we find that forces solely at k-fiber ends are sufficient to recapitulate unmanipulated k-fiber shapes, but not manipulated ones for which lateral anchorage over a 3 μm length scale near chromosomes is also essential. Such anchorage robustly preserves k-fiber orientation near chromosomes while allowing pivoting around poles. Anchorage over a shorter length scale cannot robustly restrict pivoting near chromosomes, while anchorage throughout the spindle obstructs pivoting at poles. Together, this work reveals how spatially regulated anchorage gives rise to spatially distinct mechanics in the mammalian spindle, which we propose are key for function.
Redox regulation of yeast Hsp70 modulates protein quality control while directly triggering an Hsf1-dependent cytoprotective response
Authors: Santiago Alec , Morano Kevin A. 
Published: April 2022

AbstractNeurodegenerative disease affects millions of Americans every year, through diagnoses such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. One factor linked to formation of these aggregates is damage sustained to proteins by oxidative stress. Cellular protein homeostasis (proteostasis) relies on the ubiquitous Hsp70 chaperone family. Hsp70 activity has been previously shown to be modulated by modification of two key cysteines in the ATPase domain by oxidizing or thiol-modifying compounds. To investigate the biological consequences of cysteine modification on the Hsp70 Ssa1 in budding yeast, we generated cysteine null (cysteine to serine) and oxidomimetic (cysteine to aspartic acid) mutant variants of both C264 and C303 and demonstrate reduced ATP binding, hydrolysis and protein folding properties in both the oxidomimetic as well as hydrogen peroxide-treated Ssa1. In contrast, cysteine nullification rendered Ssa1 insensitive to oxidative inhibition. The oxidomimetic ssa1-2CD (C264D, C303D) allele was unable to function as the sole Ssa1 isoform in yeast cells and also exhibited dominant negative effects on cell growth and viability. Ssa1 binds to and represses Hsf1, the major transcription factor controlling the heat shock response, and the oxidomimetic Ssa1 failed to stably interact with Hsf1, resulting in constitutive activation of the heat shock response. Consistent with the in vitro findings, ssa1-2CD cells were compromised for de novo folding, post-stress protein refolding and in regulated degradation of a model terminally misfolded protein. Together these findings pinpoint Hsp70 as a key link between oxidative stress and proteostasis, information critical to understanding cytoprotective systems that prevent and manage cellular insults underlying complex disease states.
Service guarantees in an e-commerce platform: proposition of a framework based on customers' expectations, negative experiences and behavioural responses
Authors: Oliveira Alessandro Silva de , Souki Gustavo Quiroga , Silva Dirceu da , Rezende Daniel Carvalho de , Batinga Georgiana Luna 
Published: April 2022

PurposeCompanies' relationship with their customers through e-commerce platforms has increased considerably in the past few years, bringing new challenges concerning service guarantees (SG). This study aims to propose a framework of the relations between customers' expectations on SG, their negative experiences and their attitudes and behavioural intentions towards an e-commerce platform.Design/methodology/approachThe research had a qualitative and descriptive approach. Testimonials from clients of an online e-commerce platform were obtained through interviews via videoconference and non-participant observation on a complaints website in Brazil. The testimonies were analysed through content analysis.FindingsThe customer expectations regarding the SG offered by the e-commerce platform are congruent with the five categories of the theory that support this research. Customer testimonials on the complaints site show that their negative experiences with the e-commerce platform generated negative emotional, cognitive and behavioural responses towards the company. A framework was proposed, including customers' expectations regarding SG, their negative experiences and their repercussions on clients' attitudes and behavioural intentions.Originality/valueThis article is the only that contemplates customers' expectations about SG in an e-commerce platform, relating them to attitudes and behavioural intentions. Thus, its framework demonstrates the relationships between customer expectations about SGs, their negative experiences and attitudinal and behavioural repercussions. This article brings academic and managerial contributions for companies and managers of e-commerce platforms. It contributes to clients and consumer protection associations by revealing problems they face with SG on e-commerce platforms. This research can be used by those responsible for elaborating laws and public policies to regulate and inspect the relationships between e-commerce platforms and their customers.
Further evolving the critical incident technique (CIT) by applying different contemporary approaches for analyzing qualitative data in CIT studies
Authors: Watkins Karen E. , Ellinger Andrea D. , Suh Boyung , Brenes-Dawsey Joseph C. , Oliver Lisa C. 
Published: April 2022

Purpose
The critical incident technique (CIT) is widely used in many disciplines; however, scholars have acknowledged challenges associated with analyzing qualitative data when using this technique. Therefore, the purpose of this article is to address the data analysis issues that have been raised by introducing some different contemporary ways of analyzing qualitative critical incident data drawn from recent dissertations conducted in the human resource development (HRD) field.

Design/methodology/approach
This article describes and illustrates different contemporary qualitative re-storying and cross-incident analysis approaches with examples drawn from previously and recently conducted qualitative HRD dissertations that have used the CIT.

Findings
Qualitative CIT analysis comprises two processes: re-storying and cross-incident analysis. The narrative inquiry–based re-storying approaches the authors illustrate include poetic narrative and dramatic emplotting. The analytical approaches we illustrate for cross-incident analysis include thematic assertion, grounded theory, and post-structural analysis/assemblages. The use of the aforementioned approaches offers researchers contemporary tools that can deepen meaning and understanding of qualitative CIT data, which address challenges that have been acknowledged regarding the difficulty of analyzing CIT data.

Research limitations/implications
The different contemporary qualitative approaches that we have introduced and illustrated in this study provide researchers using the CIT with additional tools to address the challenges of analyzing qualitative CIT data, specifically with regard to data reduction of lengthy narrative transcripts through re-storying as well as cross-incident analyses that can substantially deepen meaning, as well as build new theory and problematize the data through existing theory.

Practical implications
A strength of the CIT is its focus on actual events that have occurred from which reasoning, behaviors, and decision-making can be examined to develop more informed practices.

Originality/value
The CIT is a very popular and flexible method for collecting data that is widely used in many disciplines. However, data analysis can be especially difficult given the volume of narrative qualitative data that can result from data collection. This paper describes and illustrates different contemporary approaches analyzing qualitative CIT data, specifically the processes of re-storying and cross-incident analysis, to address these concerns in the literature as well as to enhance and further evolve the use of the CIT method.
Barriers and reliability of patient experience evaluation in Ontario: perspectives of healthcare providers, managers, and policymakers
Authors: Zakkar Moutasem A. , Janes Craig , Meyer Samantha 
Published: April 2022

PurposePatient experience (PE) evaluation can identify critical issues in healthcare quality. Various methods are used for PE evaluation in the healthcare system in Ontario; however, evidence suggests that PE evaluation is not systematically performed and has not received substantial buy-in from healthcare providers. This study explores the perspectives of healthcare providers, managers and policymakers in Ontario on PE evaluation methods, barriers, utility and reliability.Design/methodology/approachThe study used a qualitative descriptive design. Twenty-one semistructured interviews were conducted with healthcare providers, managers and policymakers in Ontario between April 2018 and May 2019. The authors used thematic analysis to analyze the data. The Consolidated Criteria for Reporting Qualitative Research quality criteria were used.FindingsBarriers to PE evaluation include evaluation cost and the time and effort required to collect and analyze the data. Several factors affect the reliability of the evaluation, resulting in an unrealistically high level of patient satisfaction. These include the inclusivity of evaluation, the subjective nature of patient feedback, patients' concerns about health service continuity and the anonymity of evaluation. Participants were skeptical about the meaningfulness of evaluation because it may only yield general information that cannot be acted upon by healthcare providers, managers and policymakers for quality improvement.Originality/valueThis paper reveals that many healthcare providers, managers and policymakers do not see a tangible value in PE evaluation, regardless of Ontario's patient-centeredness and “patient first” rhetoric. An improvement in evaluation methods and a cultural change in the healthcare system regarding the value of PE are required to foster a better appreciation of the benefits of PE evaluation.
How university students are taught about sustainability, and how they want to be taught: the importance of the hidden curriculum
Authors: Turner Lucy Millicent , Hegde Smitha , Karunasagar Indrani , Turner Rebecca 
Published: April 2022

PurposeIndia is unique, having enshrined in law the teaching of sustainability education (SE) within all levels of formal education. This study aims to examine the integration and perceptions of SE within the higher education (HE) sector in India and identify any lessons that can be exported about the teaching of SE from the Indian HE environment.Design/methodology/approachFocusing on a science-based teaching and research institute at a private university in India, a quantitative, cross-sectional study examined the extent to which SE was integrated into the university and how it was perceived by students and staff. Data were collected through two online questionnaires administered to lecturers and undergraduate students during the 2017 academic year.FindingsMost students reported that their university experiences had contributed significantly to their knowledge about sustainability. Results also showed there was a positive association between the teaching and learning about sustainability, although staff and students reported that this could be improved by including more active, student-centred teaching and learning approaches. However, students felt that they had learnt the most about sustainability from the informal “hidden” rather than the “formal” curriculum. This suggests that research is now required into ways to capitalise on this as a medium to further develop, not just Indian, but students’ worldwide sustainability literacy.Originality/valueTo the best of the authors’ knowledge, this paper is the first to present a detailed study of the perceptions of the contribution of the “formal” and the informal “hidden” curriculum to SE by students and staff at an Indian university.
Consumers' willingness to share digital footprints on social media: the role of affective trust
Authors: Muhammad Syed Sardar , Dey Bidit Lal , Syed Alwi Sharifah Faridah , Kamal Muhammad Mustafa , Asaad Yousra 
Published: April 2022

PurposeDespite consumers' widespread use of social media platforms, there is scant research on the underlying factors that influence their willingness to share digital footprints on social media. The purpose of this study is to address this research gap by examining consumers' cognitive and affective attitudes simultaneously.Design/methodology/approachThis research used quantitative method by using online survey administered to a sample of 733 social media users.FindingsThe findings indicate both cognitive and affective attitudes jointly influence consumers' behavioural intentions with trust as a key construct mediating the relationship between attitudinal antecedents and consumers' willingness to share digital footprints on social media.Research limitations/implicationsThis study contributes to the information systems (IS) literature by offering a comprehensive framework constituting the joint attitudinal components as antecedents to consumers' behavioural intention for sharing digital footprints while trust works as a mediator.Practical implicationsThis paper has important managerial implications. It helps marketers and IS managers in profiling consumers, understanding consumption patterns, sharing of digital footprints, which are useful for effective market segmentation, product development and future design of social media platforms. It informs social media providers of the importance of not only focussing on functional aspects but also underscores the essence of paying attention to consumers' affect towards social media platforms, especially trust.Originality/valueThe paper presents an original framework that explains the influence of joint attitudinal components on behavioural intention, with trust as a mediator.
A review of business model research: what next for industrial marketing scholarship?
Authors: Coombes Philip 
Published: April 2022

Purpose
The purpose of this paper is to present the findings of a bibliometric analysis of the evolution and structure of business model research in industrial marketing scholarship during the period between 2011 and 2020 and to discuss potential directions for future empirical research.

Design/methodology/approach
Bibliometric methodologies are deployed to objectively evaluate the business model research that has made the most impact within industrial marketing scholarship as well as the prominent scholars and key topics driving the discipline at points in time.

Findings
The findings demonstrate the formative but increasing engagement that industrial marketing scholarship has had with business model literature and the limited but increasing degree that business models have influenced industrial marketing literature. Potential directions for the empirical development of business model literature are argued to lie in the areas of collaboration and coopetition by examining the notion of value within the relationships, interactions and/or networks evidenced in European seaports business models.

Research limitations/implications
Bibliometric analysis is retrospective in nature so developments in the literature appear only after some time has elapsed. Different keyword selection when formulating search strings for sampling may have brought some deviations in the analysis.

Originality/value
Research that investigates the link between business models and industrial marketing is still scarce. This paper is among the few that analyze objectively the evolution and structure of business model literature in industrial marketing scholarship from a longitudinal perspective with a particular emphasis on the period between 2011 and 2020.
Integrating Syrian refugee workers in global supply chains: creating opportunities for stable trade
Authors: Cole Rosanna , Al-Ma'aitah Noor , Al Hasan Rima 
Published: April 2022

PurposeThis paper presents an empirical study of a Syrian refugee workforce in textile export from Jordan. The purpose of this study is to determine the challenges of integrating Syrian refugees into the local workforce and to consider the implications of these challenges for the global supply chain aiming to create stable trade.Design/methodology/approachData were collected via three face-to-face focus group interviews with refugee workers and managers at a clothing factory site in Al-Duleil, Zarqa. Data were analysed using the Gioia methodology.FindingsWorker attitudes, factory environment, and government support are important factors for refugees' workforce participation. The success of integrating Syrian refugees into the Jordanian workforce was largely a matter of their attitudes, commitment and motivations for taking up manufacturing work. Misconceptions about the roles refugees will undertake were identified, which results in fewer people becoming self-sufficient through employment.Research limitations/implicationsThis research contributes to understanding refugees' long-term integration in the host country by investigating refugee workers' and their managers' perspectives. Considering the views of multiple stakeholders enrichs the literature on refugees' integration.Social implicationsUnderstanding refugees' perspectives facilitates their integration in the host country which leads to improvement in their wellbeing and quality of life. More broadly, Jordan's approach to integrating refugees into the economy is seen as a development opportunity rather than a crisis to be handled.Originality/valueRather than focus on the procedures of integrating refugees in the host country, this study provides the voices of refugee migrant workers themselves, thereby offering a more complete picture of those factors shaping refugees' (dis)integration in local communities.
How can leaders help? A mediated moderation influence of leader consideration and structure initiation on employee learning from work failures
Authors: Zhou Qiwei , Mao Jih-Yu , Xiang Shuting , Huang Ran , Liu Bowei 
Published: April 2022

Purpose
Encountering work failures is not uncommon for employees. Employee learning from work failures is critical to knowledge management and employee development. This study aims to examine leaders’ role in facilitating employee learning from work failures.

Design/methodology/approach
A two-wave survey featuring full-time employees in China was conducted to test the hypotheses. Linear regression analyses were adopted.

Findings
Leader consideration and leader structure initiation are positively related to employee learning from work failures. Leader structure initiation strengthens the positive relationship between leader consideration and employee learning from work failures. Employee intrinsic motivation mediates the interactive effect of leader consideration and leader structure initiation on employee learning from work failures, such that leader structure initiation strengthens the positive relationship between leader consideration and employee learning from work failures through employee intrinsic motivation.

Originality/value
Despite the importance of employee learning from work failures, little is known about leaders’ role in facilitating such behavior. Whereas leader consideration and structure initiation are distinct and sometimes even competing, this study suggests that they complement each other to exert positive influences on employee learning from work failures. In addition, this study identifies an underlying influence mechanism.
Exploring the leadership development journey of SME owner-managers
Authors: Vuhuong Jen , Edwards Gareth 
Published: April 2022

PurposeThis research aimed to investigate influences on and opinions of leadership development in small and medium-sized enterprise (SME) business owner-managers. Therefore, the objective of this study was to respond to the research question – How do SMEs’ founder-owner-managers develop their leadership over time?Design/methodology/approachThis study applies a history narrative qualitative method to explore the leadership development journey of SMEs’ founder-owner-managers throughout their lifetime. Fifteen founder-owner-managers were interviewed.FindingsFive main themes emerge reflecting a social contextual process starting from early childhood: (1) the dominant influence of parents on leadership qualities and behaviours; (2) the importance of sports activities in shaping leadership qualities and identities; (3) the dominant influence of role models especially bad role models on leadership perceptions and behaviours; (4) the importance of self-learning, experimentation and self-reflection in developing entrepreneurship capability and (5) the importance of community-based social networks in gaining support and practicing leadership capability.Originality/valueThe originality of this research lies within the methodology used whereby a history narrative qualitative method is employed to develop data for analysis purposes. Using this methodology, this study contributes to a broader understanding of SMEs’ founder-owner-managers’ leadership development journey by taking a more expansive view to explore the development process throughout their lifetime.