bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of the COVID-19 pandemic on ovarian tissue cryopreservation planning in the Bologna clinical center
Authors: Magnani Valentina , Vicenti Rossella , Fabbri Raffaella , Paradisi Roberto , De Meis Lucia , Raimondo Diego , Casadio Paolo , Seracchioli Renato 
Published: December 2022

IntroductionTreatment of patients with COVID-19 has been a priority by competing with the treatment of any other disease due to limited hospital resources. The current pandemic situation has focused the attention of healthcare providers around the world away from all other non-emergency health problems, including oncofertility. The aim of the study was to evaluate the repercussions of the COVID-19 pandemic on the activity levels of ovarian tissue cryopreservation (OTC) in the our center.MethodsThe study analyzed the number of patients treated for OTC in our center during three periods: pre-pandemic period: March 2019-February 2020, pandemic period: March 2020-February 2021 and post-pandemic period: March 2021-February 2022.ResultsIn our center routine hospital operation was completely reorganized, allowing only urgent interventions. Continuing to urgently preserve fertility during the pandemic required rapid changes to our standard practices for the care of these vulnerable patients. Despite the modifications, there was no difference in the number of OTC performed among the periods analyzed. Similarly, the number of patients who did not perform OTC was the same over the three years analyzed.DiscussionDespite the local and national restructuring of care to conserve resources and protect the community, it is significant to continue offering fertility-sparing treatment to cancer patients. This emphasis on the importance of preserving fertility despite the pandemic further highlights the essential and urgent nature of this procedure.
Characterization of orthotopic xenograft tumor of glioma stem cells (GSCs) on MRI, PET and immunohistochemical staining
Authors: Liu Yufei , Zhu Wenzhen , Zhu Hongquan , Zhang Jiaxuan , Zhang Ju , Shen Nanxi , Jiang Jingjing , Xue Yunjing , Jiang Rifeng 
Published: December 2022

IntroductionThe orthotopic xenograft tumors of human glioma stem cells (GSCs) is a recent glioma model with genotype and phenotypic characteristics close to human gliomas. This study aimed to explore the imaging and immunohistochemical characteristics of GSCs gliomas.MethodsThe rats underwent MRI and 18F-FDG PET scan in 6th–8th weeks after GSCs implantation. The MRI morphologic, DWI and PET features of the tumor lesions were assessed. In addition, the immunohistochemical features of the tumor tissues were further analyzed.ResultsTwenty-five tumor lesions were identified in 20 tumor-bearing rats. On structural MRI, the average tumor size was 30.04±17.31mm2, and the intensity was inhomogeneous in 76.00% (19/25) of the lesions. The proportion of the lesions mainly presented as solid, cystic and patchy tumor were 60.00% (15/25), 16.00% (4/25) and 24.00% (6/25), respectively. The boundary was unclear in 88.00% (22/25), and peritumoral mass effect was observed in 92.00% (23/25) of the lesions. On DWI, 80.00% (20/25) of the lesions showed increased intensity. Of the 14 lesions in the 11 rats underwent PET scan, 57.14% (8/14) showed increased FDG uptake. On immunohistochemical staining, the expression of Ki-67 was strong in all the lesions (51.67%±11.82%). Positive EGFR and VEGF expression were observed in 64.71% (11/17) and 52.94% (9/17) of the rats, whereas MGMT and HIF-1α showed negative expression in all the lesions.DiscussionGSC gliomas showed significant heterogeneity and invasiveness on imaging, and exhibited strong expression of Ki-67, partial expression of EGFR and VEGF, and weak expression of MGMT and HIF-1α on immunohistochemical staining.
Case report: The art of anesthesiology—Approaching a minor procedure in a child with MPI-CDG
Authors: Chang En-Che , Chang Yu-Hsuan , Tsai Yu-Shiun , Hung Yi-Li , Li Min-Jia , Wong Chih-Shung 
Published: December 2022

Background: Protein glycosylation plays an important role in post-translational modification, which defines a broad spectrum of protein functions. Accordingly, infants with a congenital disorder of glycosylation (CDG) can have N-glycosylation, O-glycosylation, or combined N- and O-glycosylation defects, resulting in similar but different multisystem involvement. CDGs can present notable gastrointestinal and neurologic symptoms. Both protein-losing enteropathy and hypotonia affect the decision of using anesthetics. We reported a case of MPI-CDG with protein-losing enteropathy and muscular hypotonia that underwent different anesthesia approach strategies of vascular access. Here, we highlight why intubation with sevoflurane anesthesia and sparing use of muscle relaxants is the optimal strategy for such a condition.Case presentation: A 25-month-old girl, weighing 6.6 kg and 64 cm tall, suffered chronic diarrhea, hypoalbuminemia, and hypotonia since birth. Protein-losing enteropathy due to MPI-CDG was documented by whole-exome sequencing. She underwent three sedated surgical procedures in our hospital. The sedation was administered twice by pediatricians with oral chloral hydrate, intravenous midazolam, and ketamine, to which the patient showed moderate to late recovery from sedation and irritability the following night. The most recent one was administered by an anesthesiologist, where endotracheal intubation was performed with sevoflurane as the main anesthetic. The patient regained consciousness immediately after the operation. She had no complications after all three sedation/anesthesia interventions and was discharged 7 days later, uneventful after the third general anesthesia procedure.Conclusion: We performed safe anesthetic management in a 25-month-old girl with MPI-CDG using sevoflurane under controlled ventilation. She awoke immediately after the procedure. Due to the disease entity, we suggested bypassing the intravenous route to avoid excess volume for drug administration and that muscle relaxant may not be necessary for endotracheal intubation and patient immobilization when performing procedures under general anesthesia in CDG patients.
Endocannabinoid metabolism inhibition has no effect on spontaneous fear recovery or extinction resistance in Lister hooded rats
Authors: Warren William G. , Papagianni Eleni P. , Hale Ed , Brociek Rebecca A. , Cassaday Helen J. , Stevenson Carl W. 
Published: December 2022

Endocannabinoid transmission is emerging as a target for treating anxiety-related disorders, given its regulation of fear extinction. Boosting anandamide levels via inhibition of its metabolism by fatty acid amide hydrolase (FAAH) can enhance extinction, whereas inhibiting monoacylglycerol lipase (MAGL) to elevate 2-arachidonoylglycerol levels can impair extinction. However, whether endocannabinoids regulate fear relapse over time or extinction resistance remains unclear. In two experiments using auditory fear conditioned rats, we examined the effects of the FAAH inhibitor URB597 and the MAGL inhibitor JZL184 administered systemically on 1) spontaneous fear recovery after delayed extinction, and 2) extinction resistance resulting from immediate extinction [the immediate extinction deficit (IED)]. In Experiment 1, URB597 or JZL184 was given immediately after delayed extinction occurring 24 h after conditioning. Extinction recall and spontaneous fear recovery were tested drug-free 1 and 21 days later, respectively. We found no effects of either drug on extinction recall or spontaneous fear recovery. In Experiment 2, URB597 or JZL184 was given before immediate extinction occurring 30 min after conditioning and extinction recall was tested drug-free the next day. We also examined the effects of propranolol, a beta-adrenoceptor antagonist that can rescue the IED, as a positive control. JZL184 enhanced fear expression and impaired extinction learning but we found no lasting effects of URB597 or JZL184 on cued extinction recall. Propranolol reduced fear expression but, unexpectedly, had no enduring effect on extinction recall. The results are discussed in relation to various methodological differences between previous studies examining endocannabinoid and adrenergic regulation of fear extinction.
Simulation of hybrid silicon nitride/polymer Mach-Zehnder optical modulator beyond 170 GHz
Authors: Huang Beiju , Zhang Zanyun , Li Meixin , Zhang Kaixin , Liu Tianjun , Jiang Hao , Wang Qixin , Xing Jiaming 
Published: December 2022

In this paper, a hybrid Mach-Zehnder optical modulator is proposed based on silicon nitride/organic polymer waveguides, which is expected to break through the performance bottleneck of the silicon-based optical modulator by exploiting the low-loss optical transmission property of silicon nitride waveguides and the excellent modulation performance of organic polymers. For reduction of the optical loss and ease of photonic packaging, perfectly vertical silicon nitride bidirectional grating couplers are utilized for both input/output optical coupling and power splitting/combining. Thus, a Mach-Zehnder interferometer can be constructed with a back-to-back configuration of such grating couplers. With grating apodization, the coupler can achieve a simulated coupling efficiency of 70%. To bridge the silicon nitride waveguides and the polymer waveguides, a longitudinal adiabatic mode-spot converter with a transmission efficiency of 99.2% was designed. In this paper, high-β donor-π bridge-accepter molecule YLD-124 combined with HD-BB-OH as the host polymer is utilized for a design example. The polymer waveguides with inverted ridge structure can be realized through the processes of silica cladding etching, spin coating or microfluidic trench filling of polymer. Following this design, we numerically demonstrate a hybrid silicon nitride-polymer Mach-Zehnder modulator with modulation efficiency of 1.57 Vcm and Electric-Optical bandwidth of 174 GHz. The total insertion loss is less than 5.74 dB, including two grating coupler losses of about 3.08 dB.
Grafting promoted antioxidant capacity and carbon and nitrogen metabolism of bitter gourd seedlings under heat stress
Authors: Liang Le , Tang Wen , Lian Huashan , Sun Bo , Huang Zhi , Sun Guochao , Li Xiaomei , Tu Lihua , Li Huanxiu , Tang Yi 
Published: December 2022

IntroductionHeat stress can limit vegetable growth, and this can lead to constraints on agricultural production. Grafting technologies, however, can be used to alleviate various plant stresses.MethodsIn this study, the differences in the heat stress impacts and recovery abilities of pumpkin and luffa rootstocks for bitter gourd were analyzed in terms of their antioxidant activity and carbon and nitrogen metabolism.ResultsCompared with the un-grafted and self-grafted bitter gourd, which suffered from heat stress at 40°C for 24 h, heterologously grafted bitter gourd showed higher heat stability of the cell membrane (relative conductivity and malondialdehyde content were reduced), reduced oxidative stress (antioxidant enzyme activity was increased and the reactive oxygen species content reduced), and increased enzyme activity (sucrose phosphate synthase, sucrose synthase, neutral invertase, and acid invertase) and sugar content (soluble sugar, sucrose, fructose, and glucose) in carbon metabolism. The enzyme activity (nitrate reductase, nitrite reductase, and glutamine synthetase) and product content (nitrate and nitrite) of nitrogen metabolism were also found to be increased, and this inhibited the accumulation of ammonium ions. After the seedlings were placed at 25°C for 24 h, the heterogeneous rootstocks could rapidly restore the growth of the bitter gourd seedlings by promoting the antioxidant and carbon and nitrogen metabolism systems. When luffa was used as rootstock, its performance on the indexes was better than that of pumpkin. The correlation between the various indicators was demonstrated using a principal component and correlation analysis.DiscussionThe luffa rootstock was found to be more conducive to reducing cell damage and energy loss in bitter gourd seedlings caused by heat induction through the maintenance of intracellular redox homeostasis and the promotion of carbon and nitrogen metabolism.
Baseline emotional state influences on the response to animated short films: A randomized online experiment
Authors: Negrão Juliana Gioia , Bazán Paulo Rodrigo , de Azevedo Neto Raymundo Machado , Lacerda Shirley Silva , Ekman Eve , Kozasa Elisa Harumi 
Published: December 2022

IntroductionConsidering the relevance of the emotional state, it is necessary to understand how daily stimuli can modulate the emotions. Animated short films are common stimuli, but it is unknown how they can modulate the emotional state. The study aimed to evaluate: how participants’ emotional state changed after watching animated short films with positive or negative emotional valence in an online experiment; the relationship between participants’ baseline score on an Emotional Intensity Scale and their potential change in the main emotion after watching the films; and the association between the initial main emotion valence and the potential change in this emotion with participants’ sociodemographic information.MethodsA sample of 2,269 participants recruited during COVID-19 pandemic were randomly assigned to either watch a negative or positive animated short film.ResultsThe results showed that, after watching a film with negative valence, participants were in a more negative emotional state than at baseline and compared with those who watched the film with positive valence. Also, individuals who had a negative baseline emotion and maintained the same emotion after the film had presented higher baseline emotional state scores (more negative emotion) than those who changed their emotions. In addition, the individuals who kept the baseline emotion had an association with age, marital status, level of education and psychiatric disorders, use of medication, and emotional awareness, while the individuals who changed the baseline emotion had an association with age, gender, and following or not social distancing recommendations.ConclusionBaseline emotional state may influence the response to animated short films and sociodemographic characteristics are associated with the initial main emotion valence and its potential change in this emotion.
Alterations of theta power and synchrony during encoding in young adult binge drinkers: Subsequent memory effects associated with retrieval after 48 h and 6 months
Authors: Huang Siyuan , White David R. , Marinkovic Ksenija 
Published: December 2022

IntroductionYoung emerging adults commonly engage in binge drinking which is associated with a range of neurocognitive deficits, including memory impairments. However, evidence on neural oscillations mediating episodic memory in this population is lacking. To address this gap, we recorded theta oscillatory activity in young binge (BDs) and light drinkers (LDs) during memory encoding and analyzed it prospectively as a function of subsequent retrieval. Theta underlies successful encoding of novel items in memory through corticolimbic integration. Subsequent memory effects (SMEs) are reflected in stronger theta activity during encoding of the items that are later remembered compared to those that are later forgotten.MethodsIn the present study, 23 BDs (age: 23.3 ± 3.3) and 24 LDs (age: 23.4 ± 3.3) rated emotionally evocative images with negative, positive, and neutral themes during implicit encoding. They performed a recognition memory task on two follow-up occasions after a short (48 h), and long retention delay (6 months). Electroencephalography (EEG) signal was recorded during the encoding session and analyzed in time-frequency domain with Morlet wavelets in theta band (4–7 Hz). To evaluate SMEs, the event-related theta oscillations acquired during encoding were analyzed based on recognition outcomes after the two retention intervals.ResultsThe BD and LD groups did not differ on recognition memory. However, BDs showed attenuated event-related theta power during encoding of images that were successfully retained after 6 months compared to LDs. In addition, theta synchronous activity between frontal and left posterior regions during encoding successfully predicted recognition of the images after both retention delays in LDs but not in BDs. These SMEs on theta power and synchrony correlated negatively with high-intensity drinking in the previous 6 months. No differences between men and women were observed for any analysis.DiscussionIt has been well established that long-range neural synchrony between cortical and limbic nodes underlies successful memory encoding and retention which, in turn, depends on neural excitation/inhibition (E/I) balance. Given that binge drinking is associated with E/I dysregulation, the observed SME deficiencies are consistent with other evidence of neural hyperexcitability in BDs, and may be indicative of increased risk of developing alcohol use disorders.
Voices to be heard: Understanding family perspectives in forensic care trajectories
Authors: Rowaert Sara , De Pau Marjolein , De Meyer Florian , Nicaise Pablo , Vander Laenen Freya , Vanderplasschen Wouter 
Published: December 2022

IntroductionDe-institutionalization of psychiatric care has greatly increased the role of family members in the recovery pathways of Persons labeled as Not Criminally Responsible (PNCR). However, the role of family members in supporting PNCR in forensic psychiatric care remains understudied. Scarce evidence indicates that PNCR have to deal with stigma and endure specific burdens (i.e., symptom-specific, financial, social, and emotional). Recovery-focused research showed that recovery in both persons with a severe mental illness and family members develop in parallel with each other and are characterized by similar helpful principles (e.g., hope and coping skills). As such, the recovery pathways of PNCR often goes hand in hand with the recovery pathway of their family members. During the family recovery process, family members often experience not being listened to or being empowered by professionals or not being involved in the decision-making process in the care trajectory of their relative. Therefore, the aim of this study is to capture how family members experience the care trajectories of their relatives, more specifically by looking at family recovery aspects and personal advocacy of family members.MethodsSemi-structured interviews were conducted with 21 family members of PNCR from 14 families. A thematic analysis confirms that family members suffer from stigma and worry significantly about the future of their relative.ResultsRegarding the care trajectory of PNCR, family members experienced barriers in multiple domains while trying to support their relative: involvement in care and information sharing, visiting procedures, transitions between wards, and the psychiatric and judicial reporting by professionals. In addition, family members emphasized the importance of (social) support for themselves during the forensic psychiatric care trajectories and of a shared partnership.DiscussionThese findings tie in with procedural justice theory as a precondition for family support and family recovery within forensic psychiatric care.
A borderline personality assessment for adolescents: Validity and reliability of the Chinese languages borderline personality features scale (short form version) for adolescents/children
Authors: Zhuo Chuanjun , Chen Guangdong , Lin Chongguang , Jia Feng , Yang Lei , Zhang Qiuyu , Chen Jiayue , Tian Hongjun , Jiang Deguo 
Published: December 2022

BackgroundBorderline personality disorder (BPD) is characterized by behavioral patterns that promote suffering in many adolescents and their guardians. Currently, early diagnosis of BPD mainly depends on the effective assessment of pathological personality traits (i.e., borderline personality features) and using the indicated scales. The Borderline Personality Features Scale for Children-Short Form (BPFSC-SF) is widely used and the introduction of a Chinese version of the BPFSC-SF, can improve the diagnosis and prognosis of Chinese patients with BPD.ObjectiveThe aim of the present study was to assess the validity and reliability of the Chinese version of the BPFSC-SF.Method120 adolescents with BPD were enrolled in the present study and completed the BPFSC-SF and the Personality Belief Questionnaire-Short Form (PBQ-SF) assessments. Confirmatory factor analysis (CFA) was used to test assessment validity. Test-retest correlations and the Cronbach's α coefficients were used to determine reliability.ResultsCFA analysis identified primary factors of BPFSC, with each item ranging from 0.597~0.899. The Spearman rank correlation coefficient was 0.877 between CL-BFSFC-SF and the state vs. trait loneliness scale. The Cronbach's α of the scale was 0.854 in the clinical group. The test-retest reliability correlation coefficient (interclass correlation coefficients.ICC) was 0.937.ConclusionThe Chinese version of BPFSC-SF is a valid and reliable tool for adolescent Chinese patients with BPD.
Exploring the predictive properties of the Hayes Ability Screening Index subtest background information in identifying individuals with MBID among in-patients with SUD
Authors: Braatveit Kirsten J. , Assmus Jörg , Hove Oddbjørn 
Published: December 2022

IntroductionFor individuals with substance use disorder (SUD), mild to borderline intellectual disability (MBID) goes undetected in treatment clinics. The Hayes Ability Screening Index (HASI) has been found to be a valid, time-saving screening instrument for MBID in SUD treatment. MBID can have significant implications for treatment planning and outcomes. Therefore, it is important to have methods for the early recognition of these comorbid conditions. Because of less sensitivity to recent or ongoing substance use, the HASI subtest background information may be particularly valuable as an early screening of MBID. The main aim was to investigate the convergent, predictive, and discriminant validity of the HASI subtest background information in identifying in-patients with SUD as MBID or non-MBID.MethodsEighty-four in-patients with SUD aged 19–64 participated in this multicentre study. MBID was diagnosed according to the ICD-10 using WAIS-IV, Vineland II, and self-reported childhood learning difficulties.ResultsThe main finding was that, among the HASI subtests, background information was the strongest predictor. A HASI background information cut between 6 and 7 showed a sensitivity of 78% and a specificity of 72%.ConclusionThe HASI subtest background information has acceptable convergent, predictive, and discriminant validity as a screening for MBID among in-patients in SUD treatment.