bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromosomal position of ribosomal protein genes impacts long term evolution ofVibrio cholerae
Authors: Larotonda Leticia , Mornico Damien , Khanna Varun , Bernal Joaquín , Ghigo Jean Marc , Val Marie-Eve , Comerci Diego , Mazel Didier , Soler-Bistué Alfonso 
Published: May 2022

AbstractIt is unclear how gene order within the chromosome influences bacterial evolution. The genomic location of genes encoding the flow of genetic information is biased towards the replication origin (oriC) in fast-growing bacteria. To study the role of chromosomal location on cell physiology we relocated theS10-spec-αlocus (S10), harboring half of ribosomal protein genes, to different chromosomal positions in the fast-growing pathogenV. cholerae. We found that growth rate, fitness and infectivity inversely correlated the distance between S10 andoriC. To gain insight into the evolutionary effect of ribosomal protein genomic position, we evolved strains bearing S10 at its currentoriC-proximal location or derivatives where the locus far from it, at the chromosomal termini. All populations increased their growth rate along the experiment regardless S10 genomic location. However, the growth rate advantage of anoriC-proximal location persisted along experimental evolution indicating that suppressor mutations cannot compensate S10 genomic position. An increment in biofilm forming capacity was another common trait observed along the experiment. Deep sequencing of populations showed on average 1 mutation fixed each 100 generations, mainly at genes linked to flagellum biosynthesis regulation, lipopolysaccharide synthesis, chemotaxis, biofilm and quorum sensing. We selected fast-growing clones displaying a ∼10% growth rate increment. We found that they harbored inactivating mutations at, among other sites, the flagellum master regulatorsflrAB. The introduction of these mutations into naïveV. choleraestrains resulted in a ∼10% increase of growth rate. Our study therefore demonstrates that the location of ribosomal protein genes conditions the evolutionary trajectory of growth rate in the long term. While genomic content is highly plastic in prokaryotes, gene order is an underestimated factor that conditions cellular physiology and lineage evolution. The lack of suppression enables artificial gene relocation for genetic circuit reprogramming.
Hyperinsulinemia acts via acinar insulin receptors to initiate pancreatic cancer by increasing digestive enzyme production and inflammation
Authors: Zhang Anni M.Y. , Xia Yi Han , Lin Jeffrey S.H. , Chu Ken H. , Wang Wei Chuan K. , Ruiter Titine J.J. , Yang Jenny C.C. , Chen Nan , Chhuor Justin , Patil Shilpa , Cen Haoning Howard , Rideout Elizabeth J. , Richard Vincent R. , Schaeffer David F. , Zahedi Rene P. , Borchers Christoph H. , Johnson James D. , Kopp Janel L. 
Published: May 2022

SummaryThe rising pancreatic cancer incidence due to obesity and type 2 diabetes is closely tied to hyperinsulinemia, an independent cancer risk factor. Previous studies demonstrated reducing insulin production suppressed pancreatic intraepithelial neoplasia (PanIN) pre-cancerous lesions inKras-mutant mice. However, the pathophysiological and molecular mechanisms remained unknown and in particular it was unclear whether hyperinsulinemia affected PanIN precursor cells directly or indirectly. Here, we demonstrate that insulin receptors (Insr) inKrasG12D-expressing pancreatic acinar cells are dispensable for glucose homeostasis, but necessary for hyperinsulinemia-driven PanIN formation in the context of diet-induced hyperinsulinemia and obesity. Mechanistically, this was attributed to amplified digestive enzyme protein translation, triggering of local inflammation and PanIN metaplasiain vivo.In vitro, insulin dose-dependently increased acinar-to-ductal metaplasia formation in a trypsin– andInsr-dependent manner. Collectively, our data shed light on the mechanisms connecting obesity-driven hyperinsulinemia and pancreatic cancer development.
Weaving a 2D net of hydrogen and halogen bonds: cocrystal of a pyrazolium bromide with tetrafluorodiiodobenzene
Authors: van Terwingen Steven , Ebel Ben , Wang Ruimin , Englert Ulli 
Published: May 2022

Hydrohalides of Lewis bases may act as halogen bond (XB) acceptors and combine two directional interactions, namely, hydrogen bonds (HB) and XBs in the same solid. 3-(1,3,5-Trimethyl-1H-pyrazol-4-yl)acetylacetone (C11H16N2O2, HacacMePz) was protonated with HX(X = Cl or Br) to afford the hydrohalides, C11H17N2O2+·X−or H2acacMePz+·X−(1,X = Cl;2,X = Br). Hydrohalides1and2are isomorphous and adopt a classical dipole packing. Consistent with the observation for most β-diketones, the enol form with an intramolecular HB is observed. Additional noteworthy interactions are HBs of the protonated pyrazolium towards theX−anion at donor–acceptor distances of 2.9671 (17) Å for1and 3.159 (4) Å for2. Cocrystallization of hydrobromide2with the XB donor tetrafluorodiiodobenzene (TFDIB) leads to the adduct C11H17N2O2+·Br−·0.5C6F4I2·H2O or (H2acacMePz+·Br−)2·(H2O)2·TFDIB (3), in which the XB donor TFDIB is situated on a crystallographic centre of inversion. Classical HBs link organic cations, water molecules and Br−anions into chains along [010]. Almost orthogonal to this interaction, XBs with Br...I = 3.2956 (4) Å connect neighbouring chains along [102] into two-dimensional sheets in the (10-2) plane. Assisted by their negative charge, halide anions represent particularly good nucleophiles towards XB donors.
The FIX-up mix-up; undue façadism or adaptive reuse? Examining the former FIX brewery transformation into the National Museum of Contemporary Art in Athens
Authors: Tsilika Evangelia , Vardopoulos Ioannis 
Published: May 2022

PurposeThis study aims to examine the rehabilitation project of the iconic urban industrial building in Athens, “FIX” brewery, and the practices followed, so as to initiate a discussion on the role of the façades in such a process. In particular, this study suggests that by choosing to restore just two of the façades out of the whole building, while placing emphasis on creating a new face for the new use, frontality is promoted against the pre-existing homogeneity approach, and façadism is introduced. However, both façadism and frontality distance this project from the rationale behind the adaptive reuse and redevelopment of built heritage.Design/methodology/approachA thorough architectural analysis of the FIX brewery building's long history and its consecutive transformations, besides providing adequate evidence for the hypothesis set, provided the opportunity to broaden the scope of this research and explore the role of the façades in adaptive reuse practice. Methodology-wise, this research was further strengthened by a comparative analysis of the Weverij De Ploeg adaptive reuse project in Bergeijk.FindingsIn the light of this critical analysis, the current study first highlights the importance of a building's façades in shaping public perception and establishing a connection to the city, by transmitting information and meanings about the building's structure, function, character and era. Accordingly, it stretches the need for façades' retention when adapting an industrial building of cultural heritage to a new use. Second, it emphasizes the need of fostering a holistic perspective toward a historic industrial building of such merit, respecting the building as a whole and in all its depth.Originality/valueThis in-depth analysis provides a solid ground for rethinking adaptive reuse, concretizing the appropriate approaches to industrial buildings of cultural heritage from parties involved (inter aliagovernment leaders, legislators, property developers, historians, urban planners, architects and other engineers), to ensure both the building's continuity and longevity, and an efficient and sustainable urban regeneration.
A two-study examination of age discrimination, work meaningfulness, and bridge employment intentions
Authors: Peng Yisheng 
Published: May 2022

PurposeBased on the role theory, this study examines whether workplace age discrimination indirectly relates to older workers' bridge employment intentions through work meaningfulness.Design/methodology/approachStudy 1 used two-wave time-lagged survey data from one hundred and seventy nurses (≥45 years old) from the Midwestern United States. Study 2 used three-wave time-lagged survey data from one hundred and eighty-six employees from a wide range of occupations in the United States. The online survey contains various self-reports on workplace age discrimination, work meaningfulness, affective commitment, and bridge employment intentions.FindingsResults in Study 1 found that workplace age discrimination was negatively and indirectly related to older nurses' bridge employment intentions through their experiences of work meaningfulness. Results in Study 2 further confirmed the mediating role of work meaningfulness in the relationship between age discrimination and bridge employment intentions, above and beyond the role of affective commitment.Originality/valueThis study contributes to the research by testing the indirect relationship between workplace age discrimination and older workers' bridge employment intentions through work meaningfulness, further raising our awareness of the importance of social and interpersonal experiences in older workers' preretirement jobs to their late-career development.
A bibliometric and content analysis of policy instruments on facilitating the development of prefabricated construction in China
Authors: Gan Xiao-Long , Xie Kexin , Liu Hanqiu , Rameezdeen Raufdeen , Wen Tao 
Published: May 2022

PurposeThe purpose of this paper is to analyse the policy instruments used by the China's Government on prefabricated construction (PC) development, with the aim to clarify the pattern and discover the focus of the policy instruments using the methods of bibliometric analysis and content analysis.Design/methodology/approachThrough a thorough screening by all researchers, 25 policies were identified from 1995 to 2021 were selected and analysed. The external attribute analysis clarified the issuing year, organisation, and the format of each policy. The content analysis was adopted firstly to analyse the policy instrument. An analysis framework including environmental instruments, supply instruments and demand instruments was applied in categorising and counting of each policy instrument. The bibliometric analysis was then carried out to extract keywords of the policy instruments, construct a co-word network and complete the cluster analysis.FindingsThe results indicate that the policies lack consistency and had relatively low authority. The results of content analysis show that the policy system relies heavily on environmental and supply instruments, whilst the use of demand instruments seems to be grossly inadequate. The results of the bibliometric analysis identify the core ideas of each policy instrument category, discuss the existing problems and then put forward the corresponding countermeasures.Research limitations/implicationsThis study comprehensively and systematically analysed the policy instruments on facilitating the development of PC in China which broaden the research perspectives of the PC development by introducing the methods of political science. The research findings afford helpful information for future policy adjustments and elucidate the effective delivery of policies to facilitate PC development for developing countries.Originality/valueThis study provides an in-depth understanding of policy instruments used by China's Government on PC development. By integrating the policy instrument analysis framework into the mixed methods of content analysis and bibliometric analysis, this study provides an innovative way to conduct policy instrument analysis.
Disruptive technology and AI in the banking industry of an emerging market
Authors: Omoge Akinyemi Paul , Gala Prachi , Horky Alisha 
Published: May 2022

PurposeAs disruptive technologies, such as the use of artificial intelligence (AI)-enabled customer relationship management (CRM) systems, alter the processes and strategies that banks use in service delivery models, the impact of such technologies on consumer acceptance and buying behavior must continue to be examined. This research studies the impact of technology usage and acceptance of AI-enabled banking CRM systems in Nigeria on consumer buying behavior via the mediation of customer satisfaction and service quality. The study also investigates the negative impact of technology downtime, a frequent phenomenon in the emerging market, which has not, to this point, been studied on a large scale.Design/methodology/approachThe authors collect quantitative data via a face-to-face administered questionnaire from four hundred customers of ten different Nigerian banks regarding their perceptions of technology use in the banking sector.FindingsWhile the research finds that technology usage has positive and direct effects on service quality, customer satisfaction and consumer buying behavior, service quality was found not to have a significant effect on consumer buying behavior. The study also establishes that technology downtime has a moderating effect on technology usage, consumer buying behavior and customer satisfaction in the banking context.Originality/valueScant literature exists that explores the importance of culture in technology usage and acceptance, specifically in developing countries like Nigeria. This study explores the impact of technology usage along with acceptance in the Nigerian setting on Nigerian consumers and their resulting satisfaction. Technology usage has been known to impact customer satisfaction in various ways, but no study has looked specifically at how technology in the banking sector can further be of help or harm from a Nigerian perspective. This study explores the technology usage in banking sector of Nigeria and its impact on the consumer buying behaviour. No studies in our knowledge have been known to consider the role of technology downtime, a frequent phenomenon in emerging market, as a factor, which will affect the customer satisfaction and buying behavior. Thus, this study (1a) explores the negative outcomes of technology downtime on both service quality and customer satisfaction, (b) explores the moderating relationship of technology downtime on the technology usage and consumer-related outcomes.
Developing an Islamic crowdfunding model: a new innovative mechanism to finance SMEs in the Middle East
Authors: Abdeldayem Marwan , Aldulaimi Saeed 
Published: May 2022

Purpose
The purpose of this study is to investigate and discuss the viability of Islamic crowdfunding (ICF) as an alternative form of financing small and medium enterprises (SMEs) in the Middle Eastern and Islamic business environment. This study raised essential questions: what is the perception of ICF in the Middle East? Does the Middle East region really need an Islamic crowdfunding model to support SMEs? Is it possible to create a crowdfunding platform complaint with Sharia? What are the requirements for developing an Islamic crowdfunding model?

Design/methodology/approach
The methodology followed to answer these questions is a qualitative research design depends on in-depth interviews, literature review, historical analysis and critical discussion. Data analysis was conducted using NVivo to analyze 25 in-depth interviews with Islamic scholars, Sharia board members and Islamic finance experts from different Middle East countries such as Saudi Arabia, Kuwait, Egypt, Iraq, UAE and Bahrain. In addition, more than 115 transcriptions, memos and research articles were used.

Findings
The study provides a new Islamic Sharīʿah-compliant crowdfunding model as the main outcome of this study. In addition, the content analysis revealed four main themes to be the essential pillars to develop the ICF model. These provisions of Islamic Sharia are: Project Idea (Halal) (28.5%), Funding Goal (36%), Return and Risk (14%) and Funding Commitments (21.5%). The findings also revealed that the four types of crowdfunding (reward-based crowdfunding, donation-based crowdfunding, loan-based crowdfunding and equity-based crowdfunding) are legal and supported by evidence from Quran and Sunnah.

Originality/value
Despite the critical development in Islamic finance and the expanding number of young Muslims slanting digital Islamic services, empirical studies exploring this issue in the Middle East is still inadequate. Further, ICF has increased attention and there is an urgent need for financing new SMEs in the Middle East.
Identifying the key areas for benchmarking towards the improvement of small and medium scale enterprises (SMEs) construction firms in developing countries: the case of Ghana
Authors: Kissi Ernest , Aigbavboa Clinton , Babon-Ayeng Prosper 
Published: May 2022

PurposeThe purpose of this study was to identify key areas for benchmarking (BM) towards the improvement of small and medium scale enterprises (SMEs) construction firms in developing countries.Design/methodology/approachThe study employed the use of the quantitative research method in the collection and analysis of primary data collected from field surveys using a piloted close-ended questionnaire created following a review of available literature on BM. Based on 63 solicited views of professionals with SMEs (quantity surveyors, project managers and architects) data collected were statistically analysed using a one-sample t-test.FindingsThe findings of the study indicate that the key areas for BM towards the improvement of SME construction firms in developing countries in order of relevance are “Financial Performance”, “Competitiveness”, “Customer Satisfaction”, “Technology Advancement”, “Communication Skills”, “Collaboration”, “Employee Satisfaction” and “Product orientation”.Practical implicationsThe study has given more insight into the areas that need more attention for SMEs BM to achieve improvement. It can therefore be suggested firms that adapted identified areas will derive the benefits of BM. It is further opined that more BM education should be provided to various SMEs construction firms to facilitate an effective and efficient BM regime aiming at performance enhancement in project delivery.Originality/valueThis forging research attempts to identify the key areas for BM towards the improvement of SMEs construction firms in developing countries. Although there have been several efforts to create BM tools for the construction industry.
Determinants of performance drivers in online food delivery platforms: a dynamic performance management perspective
Authors: Bivona Enzo 
Published: May 2022

PurposeThis study aims to demonstrate that the Dynamic Performance Management (DPM) framework, integrating performance management with system dynamics modelling, enables decision-makers to identify sustainable strategies in online food delivery platforms, thereby avoiding company failure.Design/methodology/approachThis study undertakes a multistep methodological approach. After the literature review, a retrospective case study approach was used. To build the DPM framework and the system dynamics simulation model, primary and secondary data were collected and analysed.FindingsThis study by adopting the DPM perspective highlights the critical role performance drivers play to assess the viability of alternative growth strategies in food delivery digital platforms. As such, the findings complement extant studies which highlighted the need for adopting a dynamic perspective in Performance Measurement and Management (PMM), particularly in complex and turbulent environments. Findings also highlight that in food delivery platforms, network effects may result insufficient to reach a critical volume of users and factors such as key drivers impacting platform attractiveness must be considered to design effective PMM.Research limitations/implicationsFuture studies may apply the DPM framework here suggested to multiple digital platforms, to validate this study's findings.Practical implicationsThis paper offers a guidance to practitioners and scholars to design effective PMM in food delivery digital platforms.Originality/valueThis study offers an innovative perspective to analyse the interdependences among main mechanisms underpinning the performance of food delivery platforms. As such, it contributes to enrich prior PMM literature and addresses the call for more empirical and theoretical PMM contributions in fast-changing and turbulent environments.
The role of privacy and emotion in ARBS continuing use intention
Authors: Yin Ya , Hsu Carol 
Published: May 2022

PurposeToday, contactless businesses are becoming part of the “new normal” in daily life. Augmented reality-based services (ARBS) thus provide a mechanism for contactless commerce, offering customers access to sensory experiences, especially during the COVID-19 pandemic. However, privacy can be a key concern when consumers decide whether to continue using ARBS. Thus, drawing on the Appraisal Tendency Framework (ATF), the study aims to examine how augmentation quality (Aug-Q), discrete emotions (joy and frustration) and privacy perceptions influence users' ARBS continuing use intention.Design/methodology/approachA survey methodology with a well-designed online questionnaire was used for data collection. The data were analyzed using a structural equation model with Amos v. 22.0 software.FindingsThis study demonstrated that Aug-Q had a significant positive impact on joy and a significant negative impact on frustration. Additionally, joy was positively associated with the perception of privacy benefits and ARBS continuing use intention, while frustration was negatively associated with the perception of privacy benefits and ARBS continuing use intention. The results also indicate that (perceived privacy risks) PPR–benefits predict the likelihood of ARBS continuing use intention.Originality/valueThis study enhances understanding of users' ARBS continuing use intention from an integrative perspective based on the ATF, thus identifying the Aug-Q-induced emotions that subsequently influence privacy trade-offs and predict users' ARBS continuing use intention. The results provide evidence that privacy and emotions can be key determinants when consumers decide whether to continue using ARBS. The findings of this research may be beneficial for commercial companies in preventing the loss of ARBS users.