bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic comparison of primary human lung cells with lung tissue samples and the human A549 lung cell line highlights cell type specific responses during infections with influenza A virus
Authors: Bertrams Wilhelm , Hönzke Katja , Obermayer Benedikt , Tönnies Mario , Bauer Torsten T. , Schneider Paul , Neudecker Jens , Rückert Jens C. , Stiewe Thorsten , Nist Andrea , Eggeling Stephan , Suttorp Norbert , Wolff Thorsten , Hippenstiel Stefan , Schmeck Bernd , Hocke Andreas C. 
Published: November 2022

AbstractInfluenza A virus (IAV) causes pandemics and annual epidemics of severe respiratory infections. A better understanding of the molecular regulation in tissue and cells upon IAV infection is needed to thoroughly understand pathogenesis. We analyzed IAV replication and gene expression induced by IAV strain H3N2 Panama in isolated primary human alveolar epithelial type II cells (AECIIs), the permanent A549 adenocarcinoma cell line, alveolar macrophages (AMs) and explanted human lung tissue by bulk RNA sequencing. Primary AECII exhibit in comparison to AM a broad set of strongly induced genes related to RIG-I and interferon (IFN) signaling. The response of AECII was partly mirrored in A549 cells. In human lung tissue, we observed induction of genes unlike in isolated cells. Viral RNA was used to correlate host cell gene expression changes with viral burden. While relative induction of key genes was similar, gene abundance was highest in AECII cells and AM, while weaker in the human lung (due to less IAV replication) and A549 cells (pointing to their limited suitability as a model). Correlation of host gene induction with viral burden allows a better understanding of the cell-type specific induction of pathways and a possible role of cellular crosstalk requiring intact tissue.
Comparison of a pre-bariatric surgery very low-calorie ketogenic diet and the Mediterranean diet effects on weight loss, metabolic parameters, and liver size reduction
Authors: Erdem Nihal Zekiye , Ozelgun Demet , Taskin Halit Eren , Avsar Fatih Mehmet 
Published: November 2022

AbstractThis study compared the effects on weight as well as on metabolic parameters and liver size of a very low-calorie ketogenic diet versus a Mediterranean diet in patients with morbid obesity preparing to undergo bariatric surgery. This prospective comparison study evaluated patients 18–65 years of age who enrolled for bariatric surgery. Study duration was limited to an immediate preoperative period of 15 days. The very low-calorie ketogenic diet incorporated 10–12 kcal/kg/day of energy and 1–1.2 g/kg of protein using Kalibra (Societa Dietetica Medica) (VLCKD-SDM). The Mediterranean diet (MD) included 15–20% protein, 45–50% carbohydrate, and 25–35% fat. Changes in body mass index (BMI), liver size, and anthropometric and metabolic measurements were assessed. Between January 2016 and March 2017, of 45 patients enrolled, 30 completed the study (VLCKD-SDM, n = 15; MD, n = 15). Respective median BMI loss after VLCKD-SDM was 2.7 kg/m2 versus MD 1.4 kg/m2 (p < 0.05); median fat percentage reduction was 3.2 units versus 1.7 units (p < 0.05). Median liver size decreased 5.5% in the VLCKD-SDM group versus 1.7% in the MD group (p < 0.05). Median total cholesterol, and LDL levels decreased in both groups (p < 0.05), with greater relative decreases in the VLCKD-SDM group. Short-term preoperative diet-based weight loss in patients with morbid obesity preparing for bariatric surgery was significantly greater following a very low-calorie ketogenic diet versus a Mediterranean diet. The very low-calorie diet also significantly improved anthropometric and metabolic parameters and reduced preoperative liver size above that of the MD.
Confounding effects of heart rate, breathing rate, and frontal fNIRS on interoception
Authors: Candia-Rivera Diego , Sappia M. Sofía , Horschig Jörn M. , Colier Willy N. J. M. , Valenza Gaetano 
Published: November 2022

AbstractRecent studies have established that cardiac and respiratory phases can modulate perception and related neural dynamics. While heart rate and respiratory sinus arrhythmia possibly affect interoception biomarkers, such as heartbeat-evoked potentials, the relative changes in heart rate and cardiorespiratory dynamics in interoceptive processes have not yet been investigated. In this study, we investigated the variation in heart and breathing rates, as well as higher functional dynamics including cardiorespiratory correlation and frontal hemodynamics measured with fNIRS, during a heartbeat counting task. To further investigate the functional physiology linked to changes in vagal activity caused by specific breathing rates, we performed the heartbeat counting task together with a controlled breathing rate task. The results demonstrate that focusing on heartbeats decreases breathing and heart rates in comparison, which may be part of the physiological mechanisms related to “listening” to the heart, the focus of attention, and self-awareness. Focusing on heartbeats was also observed to increase frontal connectivity, supporting the role of frontal structures in the neural monitoring of visceral inputs. However, cardiorespiratory correlation is affected by both heartbeats counting and controlled breathing tasks. Based on these results, we concluded that variations in heart and breathing rates are confounding factors in the assessment of interoceptive abilities and relative fluctuations in breathing and heart rates should be considered to be a mode of covariate measurement of interoceptive processes.
Fibrin glue does not promote migration and proliferation of bone marrow derived mesenchymal stem cells in collagenic membranes: an in vitro study
Authors: Migliorini Filippo , Prinz Julia , Eschweiler Jörg , Schenker Hanno , Weber Christian , Maffulli Nicola , Lecouturier Sophie , Hildebrand Frank , Greven Johannes 
Published: November 2022

AbstractDuring Autologous Matrix-Induced Chondrogenesis (AMIC), the membrane is often glued into the chondral defect. However, whether fibrin glue influences cells proliferation and migration remain unclear. This study evaluated the impact of fibrin glue addition to biologic membranes loaded with bone marrow-derived mesenchymal stem cells (B-MSCs). A porcine derived collagen membrane (Cartimaix, Matricel GmbH, Germany) was used. B-MSCs were harvested from three different unrelated donors. The membranes were embedded in mounting medium with DAPI (ABCAM, Cambridge, UK) and analysed at 1-, 2-, 3-, 4-, 6-, and at 8-week follow-up. The DAPI ties the DNA of the cell nucleus, emitting blue fluorescence. DAPI/nuclei signals were analysed with fluorescence microscopy at 100-fold magnification. The group without fibrin glue demonstrated greater migration of the B-MSCs within the membrane at week 4 (P < 0.001), 6 (P < 0.001), and 8 (P < 0.001). No difference was found at week 1, 2, and 3. The group without fibrin glue demonstrated greater proliferation of B-MSCs within the membrane. These differences were significant at week 1 (P = 0.02), 2 (P = 0.008), 3 (P = 0.0009), 4 (P < 0.0001), 6 (P < 0.0001), 8 (P < 0.0001). Concluding, in the present setting, the use of fibrin in a collagenic biomembrane impairs B-MSCs proliferation and migration in vitro.
Inter-individual variability in mechanical pain sensation in patients with cervicogenic headache: an explorative study
Authors: Mingels Sarah , Dankaerts Wim , Bruckers Liesbeth , Granitzer Marita 
Published: November 2022

AbstractCurrently, evidence for effective physiotherapy interventions in patients with cervicogenic headache (CeH) is inconsistent. Although inter-individual variability in pain response is predictive for successful physiotherapy interventions, it was never explored in patients with CeH. Therefore the objective of the current study was to explore inter-individual variability in mechanical pain sensation, and its association with biopsychosocial-lifestyle (BPSL) characteristics in patients with CeH. A cross-sectional explorative analysis of inter-individual variability in mechanical pain sensation in 18 participants with CeH (29–51 years) was conducted.Inter-individual variability in mechanical pain sensation(standard deviations (SDs), F-statistics, Measurement System Analysis) was deducted from bilateral pressure pain thresholds of the suboccipitals, erector spine, tibialis anterior.BPSL-characteristicsdepression, anxiety, stress (Depression Anxiety Stress Scale-21), quality of life (Headache Impact Test-6), sleep-quality (Pittsburgh Sleep Quality Index), and sedentary time (hours/week) were questioned. Inter-individual variability in mechanical pain sensation explained 69.2% (suboccipital left), 86.8% (suboccipital right), 94.6% (erector spine left), 93.2% (erector spine right), 91.7% (tibialis anterior left), and 82% (tibialis anterior right) of the total variability in patients with CeH. The significant p-values and large F-statistic values indicate inter-individual differences in SDs. Significant associations between (1) lower quality of life and lower SDs of the suboccipital left PPT (p.005), and (2) longer sedentary time and higher SDs of the suboccipital left PPT (p.001) were observed. Results from our explorative study could suggest inter-individual variability in mechanical pain sensation at the left suboccipitals which associates with quality of life and sedentary time. These novel findings should be considered when phenotyping patients and ‘individually’ match interventions.
Essential oils block cellular entry of SARS-CoV-2 delta variant
Authors: Torres Neto Luiz , Monteiro Maria Lúcia Guerra , Fernández-Romero José , Teleshova Natalia , Sailer James , Conte Junior Carlos Adam 
Published: November 2022

AbstractAiming to fill a gap in the literature, we aimed to identify the most promising EOs blocking in vitro cellular entry of SARS-CoV-2 delta variant without conferring human cytotoxicity and provide insights into the influence of their composition on these activities. Twelve EOs were characterized by gas chromatography coupled to mass spectrometry. The antiviral and cytotoxicity activities were determined using the cell-based pseudoviral entry with SARS-CoV-2 delta pseudovirus and the XTT assay in HeLa cells expressing human angiotensin-converting enzyme 2 (HeLa ACE-2), respectively.Syzygium aromaticum,Cymbopogon citratus,Citrus limon,Pelargonium graveolens,Origanum vulgare, “Illicium verum”, andMatricaria recutitashowed EC50lowered or close to 1 µg/mL but also the lowest CC50(0.20–1.70 µg/mL), except “I. verum” (30.00 µg/mL). Among these, “I. verum”,C. limon,P. graveolensandS. aromaticumproved to be promising alternatives for SARS-CoV-2 delta variant inhibition (therapeutic index above 4), which possibly was related to the compounds (E)-anetole, limonene and beta-pinene, citronellol, and eugenol, respectively.
Has Esophageal Speech Returned as an Increasingly Viable Postlaryngectomy Voice and Speech Rehabilitation Option?
Authors: Doyle Philip C. , Damrose Edward J. 
Published: November 2022

Purpose:
The literature on postlaryngectomy voice and speech rehabilitation is long-standing. Although multiple rehabilitation options have existed over the years, the acquisition and use of esophageal speech (ES) has decreased significantly over the past 40 years. This reduction coincides with the increased application of tracheoesophageal puncture (TEP) voice restoration. The literature suggests that voice acquisition failures observed secondary to TEP may represent a similar phenomenon that led to ES acquisition failures.

Method:
A comprehensive review of the literature on ES and TEP voice/speech was conducted. Specific attention was directed toward information on ES and TEP speech failures. Information on pharyngoesophageal segment (PES) spasm in the context of ES and TEP voicing failures was of specific importance.

Results:
Similarities between voicing failures with both ES and TEP were identified. In order to resolve spasm in TEP speech, proactive efforts to eliminate it were undertaken, and regardless of the method used, voicing improvements were observed. These data suggest that both ES and TEP speech acquisition failures may be related to the same control mechanisms influencing the PES.

Conclusions:
The elimination of PES spasm provides evidence that justifies the reconsideration of ES. Consequently, ES may return as an increasingly viable postlaryngectomy voice and speech rehabilitation option.
Tribological and mechanical behaviours of resin‐based friction materials based on microcrack filling
Authors: Li Lekai , Gao Guixiong , Tong Jin , Zhuang Jian , Song Wei , Ma Yunhai , Liu Guoqin , Cao Feipeng , Yuan Shengwang , Zhang Qifeng 
Published: November 2022

AbstractTo enhance the friction performance of resin‐based friction materials, five types of specimens with different polymer ether ketone (PEEK) contents were fabricated and their physiomechanical behaviours were tested and, their tribological properties were investigated using a JF150F‐II constant‐speed tester. It was found that the addition of PEEK had a positive influence on the properties of the friction materials, and sample FM‐3 (the shorthand of ‘Friction Materials‐3’, containing 2 wt% PEEK) exhibited improved friction performance with a fade ratio and recovery ratio of 8.6% and 101.1% respectively. Among all samples, FM‐4 (the shorthand of ‘Friction Materials‐4’, containing 3 wt% PEEK) had the lowest specific wear rate with a value of 0.622 × 10−7 cm3 (N·m)−1 at 350°C. The PEEK can fill the microcracks in the composite at a high temperature and can also cover the hard abrasive particles to prevent them from directly damaging the composite. The findings from this study afford a foundation for studies to further improve the properties of resin‐based friction materials.
Liebe Leserinnen und Leser,
Authors: Treudler Regina 
Published: November 2022

Über das eigene Fachgebiet hinaus denken!Unter Komorbiditäten verstehen wir Erkrankungen, die zusätzlich zur Grundkrankheit
auftreten, und die sich deutlich auf die Gesamt-Krankheitslast bei Betroffenen
auswirken können.
Vergleichende Untersuchung der posturalen Kontrolle bei 20–40-Jährigen und Karate-Kaderathleten mittels eines neuromuskulären Trainingsgerätes
Authors: Osmanski-Zenk Katrin , Ningel Amelie , Tischer Thomas , Mittelmeier Wolfram 
Published: November 2022

Zusammenfassung

Hintergrund Die posturale Kontrolle dient dem Erhalt der aufrechten Körperhaltung und trägt zur Gleichgewichtsregulation bei. Die im höheren Alter zunehmenden neuromuskulären Defizite führen zu einer Verschlechterung der posturalen Kontrolle, die durch neuromuskuläres Training verbessert werden kann. In der Annahme, dass neuromuskuläre Defizite bereits früh auftreten, wurden die posturalen Fähigkeiten von 20–40-Jährigen untersucht. Zudem wurde der Einfluss von Leistungssport auf die posturale Kontrolle durch die Untersuchung von Karate-Kaderathleten geprüft.
Methodik Für einen Altersvergleich wurden 123 altershomogene Gesunde zwischen 20 und 40 Jahren untersucht. 24 Karate-Kaderathleten wurden mit den 20–25-jährigen Gesunden der „Normalbevölkerung“ verglichen. Zur Datenerhebung wurde ein neuromuskuläres Trainingsgerät, der Human Body Equilibrium 360 (Huber 360), verwendet.
Ergebnisse Im Altersvergleich zeigten sich für alle 7 Messungen keine signifikanten Unterschiede. Die Probanden waren zu jung, um Defizite zu erkennen. Jedoch zeigten sich einige allgemeine Erkenntnisse. Im beidbeinigen Stand war bei allen Gruppen eine signifikante Zunahme der Körperschwerpunkt (KSP) -Schwankungen nach Augenschluss zu sehen. Der Einfluss der Füßigkeit bzw. Händigkeit zeigte sich im Einbeinstand und Krafttest. Geschlechtsspezifische Unterschiede waren zu verzeichnen. Bei der Untersuchung der Karate-Kaderathleten zeigten sich verschiedene trainingsspezifische Anpassungen. So konnten Karatesportler den Wegfall der visuellen Information besser kompensieren. Karatesportler wiesen außerdem ein signifikant breiteres Gangbild und ein größeres Stabilitätsareal auf. Sportler konnten ihren KSP weiter verlagern, ohne aus dem Gleichgewicht zu geraten.
Diskussion Der Huber 360 ist als Rehabilitations- und Trainingsgerät aufgrund der geringen Trennschärfe der Tests in seinem gesamten Anwendungsspektrum nur bedingt geeignet, da signifikante Unterschiede zwischen den Gruppen schwer zu detektieren sind. Einzelne trainingsspezifische Anpassungen durch leistungsorientiertes Karatetraining sind erkennbar und unterstreichen den Einfluss des Trainings auf die posturale Kontrolle. Zusammenfassend lässt sich sagen, dass die Anwendung des Huber 360 zur Beurteilung von posturalen Fähigkeiten im Altersvergleich und bei Unterscheidung trainingsspezifischer Anpassungen einen differenzierten und selektiveren Einsatz der einzelnen Tests bedarf. Weitere Untersuchungen mit älteren Patienten mit unterschiedlichen Erkrankungen sind geplant.
Vom Nationalsozialisten zum Hitler-Erklärer: Die
autobiografischen Umdeutungen des Psychiaters Johann Recktenwald
(1882–1964)
Authors: Groß Dominik , Schmidt Mathias , Engels Pascal , Kaiser Stephanie 
Published: November 2022

Zusammenfassung
Ziel der Studie Im Mittelpunkt dieses Beitrags steht der bislang wenig
beleuchtete Psychiater Johann Recktenwald (1882–1964): Vom Vorwurf des
„Verbrechens gegen die Menschlichkeit“ freigesprochen, trat er
im Nachkriegsdeutschland mit einer neuropsychiatrischen Abhandlung über
Hitler an die Öffentlichkeit. Doch steht diese Selbstdarstellung eines
Hitler-kritischen Nervenarztes im Einklang mit den überlieferten
historischen Quellen? Wie war Recktenwalds Verhältnis zum NS-Regime, und
wie verhielt er sich im „Dritten Reich“ gegenüber den
ihm anvertrauten Patienten?
Methodik Der Aufsatz basiert im Wesentlichen auf z.T. erstmals
ausgewerteten Dokumenten verschiedenster Archive sowie auf Gerichtsprotokollen.
Diese werden ergänzt und abgeglichen mit den Schriften Recktenwalds und
der verfügbaren Sekundärliteratur.
Ergebnisse Recktenwald diente sich im „Dritten Reich“ in
mehrfacher Hinsicht dem NS-Regime an, trug Mitverantwortung für
zahlreiche Patientenmorde und rückte so in die Rolle eines
NS-Täters. Nach seinem Freispruch im Nachkriegsdeutschland war er
bestrebt, durch die kritische Auseinandersetzung mit der Psychopathologie
Hitlers eine persönliche Distanz zum Nationalsozialismus zu
konstruieren, die zugleich der eigenen Exkulpation diente.
Schlussfolgerung Recktenwald ist ein besonders eindrucksvolles Beispiel
für den Versuch von NS-Tätern, die eigene Rolle im
„Dritten Reich“ retrospektiv umzuschreiben – und
zugleich Spiegelbild einer Nachkriegsgesellschaft, die bereit war, derartige
biografische Umdeutungen zu akzeptieren, um der Aufarbeitung der
NS-Vergangenheit ausweichen zu können.
Predictive Value of Inflammatory Cytokines in Early Pregnancy for
Liver Dysfunction in Pregnant Women with Hepatitis B
Authors: Wang Wei , Jia Hongyan , Wang Yue , Sun Weize , Yang Can 
Published: November 2022

AbstractThe purpose of this study is to explore the predictive value of cytokine levels
in the first trimester of pregnancy on abnormal liver function of pregnant women
with hepatitis B in the third trimester of pregnancy. A total of 111 pregnant
women with HBV infection at 12 weeks gestation participated in the study. The
levels of IL-6, IL-8, TNF-α in peripheral blood of the patients and
liver function indexes were detected. Subsequently, the pregnant women were
followed up, and the liver function was detected at 36 weeks of gestation.
According to liver function indexes, patients were divided into normal liver
function group and abnormal liver function group to determine the correlation
between cytokines in early pregnancy and abnormal liver function in late
pregnancy. Kaplan–Meier survival curve and multivariate Cox analysis
were used to evaluate the predictive value of cytokines for liver dysfunction.
At 12 weeks of gestation, cytokine levels in the normal liver function group
were significantly lower than that in the abnormal liver function group.
Kaplan–Meier survival analysis showed that the increased IL-6 level was
associated with abnormal liver function in late pregnancy. Multivariate Cox
regression analysis revealed that IL-6 level was an independent predictor of
abnormal liver function in patients with normal liver function in the late
pregnancy. The high expression level of cytokine IL-6 at 12 weeks of pregnancy
has noteworthy predictive significance for the abnormal liver function of
hepatitis B pregnant women in third trimester of pregnancy.
Differences in color between early gastric cancer and cancer-suspected non-cancerous mucosa on linked color imaging
Authors: Kanzaki Hiromitsu , Kawahara Yoshiro , Satomi Takuya , Okanoue Shotaro , Hamada Kenta , Kono Yoshiyasu , Iwamuro Masaya , Kawano Seiji , Okada Hiroyuki 
Published: November 2022

Abstract
Background and study aims Linked color imaging (LCI) can enhance the original color of each area and may useful to detect tumorous lesions during esophagogastroduodenoscopy. However, LCI may also enhance cancer-suspected non-cancerous regional color change. We conducted a retrospective image analysis to investigate the color characteristics of early gastric cancer (EGC) and cancer-suspected non-cancerous mucosa (CSM) in LCI.
Methods LCI images of both EGC and CSM were retrospectively collected from the database of the institution. Fifteen endoscopists individually judged each image as EGC or CSM. The color difference between the inside and outside of the lesions was measured by CIE-Lab analysis in both groups and compared.
Results A total of 245 LCI images of EGC (169) and CSM (76) were extracted and randomly lined for image collection. The test by the endoscopists showed accuracy, sensitivity, and specificity of 64.0 %, 63.7 %, and 64.0 %, respectively. Although the color difference between EGC and CSM was almost the same (12.5 vs. 12.9, not significant), each parameter of ΔL (bright: –0.3 vs. –2.7, P  < 0.001), Δa (Reddish: 7.2 vs. 9.6, P = 0.004), and Δb (Yellowish: 6.4 vs. 3.8, P  < 0.001) was significantly different in the groups. The color feature of both positive ΔL and Δb to EGC showed accuracy, sensitivity, and specificity of 54.7 %, 39.6 %, 88.2%, respectively.
Conclusions The total color difference was almost the same between EGC and CSM; however, their color tones were different on linked color imaging. Although the color characteristics of EGC had high specificity, they also had low sensitivity.
Ultrasound Measurements and Physical Fitness of Elite Youth
Basketball Players
Authors: Lisón Juan Francisco , García-Herreros Sergio , Ricart Borja , Godoy Eduardo Jorge , Nozal Sara , Cotolí-Suarez Pedro , Jordán-López Jaime , Amer-Cuenca Juan José , Salvador-Coloma Pablo 
Published: November 2022

AbstractThe countermovement jump, the V-cut test, the muscle thickness and the adjacent
subcutaneous fat thickness of the gastrocnemius medialis and rectus femoris are
important physiological indicators for success in basketball. The aims of this
study were to evaluate between-age-category and between-gender differences in
these indicators and examine the relationships between physical tests and
ultrasound measurements. The measurements were recorded in a sample of 131 elite
basketball players (66 males) who played in three age-categories (U14, U16, or
U18). We performed two-way analysis of covariance tests and age-adjusted partial
correlation analyses. U16 and U18 males showed better performance in the
countermovement jump and V-cut tests and lower adjacent subcutaneous fat
thickness of the gastrocnemius medialis and rectus femoris compared to the U14
males (p≤.001) and to age-category equivalent female players
(p≤.001). Comparisons between the age categories in females did not show
significant differences in any of the study variables. Adjacent subcutaneous fat
thickness of the gastrocnemius medialis explained 22.3% of the variation
for the countermovement jump result and 12.9% of the variation for the
V-cut result in males (p<.01). This study is the first to show the
association and predictive role of subcutaneous fat thickness measured by
ultrasound in physical performance of male and female elite youth basketball
players.