bluuebunny/crossref_metadata_2025_split huggingface dataset:
Moist convection drives an upscale energy transfer at Jovian high latitudes
Authors: Siegelman Lia , Klein Patrice , Ingersoll Andrew P. , Ewald Shawn P. , Young William R. , Bracco Annalisa , Mura Alessandro , Adriani Alberto , Grassi Davide , Plainaki Christina , Sindoni Giuseppe 
Published: January 2022

AbstractJupiter’s atmosphere is one of the most turbulent places in the solar system. Whereas observations of lightning and thunderstorms point to moist convection as a small-scale energy source for Jupiter’s large-scale vortices and zonal jets, this has never been demonstrated due to the coarse resolution of pre-Juno measurements. The Juno spacecraft discovered that Jovian high latitudes host a cluster of large cyclones with diameter of around 5,000 km, each associated with intermediate- (roughly between 500 and 1,600 km) and smaller-scale vortices and filaments of around 100 km. Here, we analyse infrared images from Juno with a high resolution of 10 km. We unveil a dynamical regime associated with a significant energy source of convective origin that peaks at 100 km scales and in which energy gets subsequently transferred upscale to the large circumpolar and polar cyclones. Although this energy route has never been observed on another planet, it is surprisingly consistent with idealized studies of rapidly rotating Rayleigh–Bénard convection, lending theoretical support to our analyses. This energy route is expected to enhance the heat transfer from Jupiter’s hot interior to its troposphere and may also be relevant to the Earth’s atmosphere, helping us better understand the dynamics of our own planet.
Observation of a reduced-turbulence regime with boron powder injection in a stellarator
Authors: Nespoli F. , Masuzaki S. , Tanaka K. , Ashikawa N. , Shoji M. , Gilson E. P. , Lunsford R. , Oishi T. , Ida K. , Yoshinuma M. , Takemura Y. , Kinoshita T. , Motojima G. , Kenmochi N. , Kawamura G. , Suzuki C. , Nagy A. , Bortolon A. , Pablant N. A. , Mollen A. , Tamura N. , Gates D. A. , Morisaki T. 
Published: January 2022

AbstractIn state-of-the-art stellarators, turbulence is a major cause of the degradation of plasma confinement. To maximize confinement, which eventually determines the amount of nuclear fusion reactions, turbulent transport needs to be reduced. Here we report the observation of a confinement regime in a stellarator plasma that is characterized by increased confinement and reduced turbulent fluctuations. The transition to this regime is driven by the injection of submillimetric boron powder grains into the plasma. With the line-averaged electron density being kept constant, we observe a substantial increase of stored energy and electron and ion temperatures. At the same time, the amplitude of the plasma turbulent fluctuations is halved. While lower frequency fluctuations are damped, higher frequency modes in the range between 100 and 200 kHz are excited. We have observed this regime for different heating schemes, namely with both electron and ion cyclotron resonant radio frequencies and neutral beams, for both directions of the magnetic field and both hydrogen and deuterium plasmas.
A taxonomic, genetic and ecological data resource for the vascular plants of Britain and Ireland
Authors: Henniges Marie C. , Powell Robyn F. , Mian Sahr , Stace Clive A. , Walker Kevin J. , Gornall Richard J. , Christenhusz Maarten J. M. , Brown Max R. , Twyford Alex D. , Hollingsworth Peter M. , Jones Laura , de Vere Natasha , Antonelli Alexandre , Leitch Andrew R. , Leitch Ilia J. 
Published: January 2022

AbstractThe vascular flora of Britain and Ireland is among the most extensively studied in the world, but the current knowledge base is fragmentary, with taxonomic, ecological and genetic information scattered across different resources. Here we present the first comprehensive data repository of native and alien species optimized for fast and easy online access for ecological, evolutionary and conservation analyses. The inventory is based on the most recent reference flora of Britain and Ireland, with taxon names linked to unique Kew taxon identifiers and DNA barcode data. Our data resource for 3,227 species and 26 traits includes existing and unpublished genome sizes, chromosome numbers and life strategy and life-form assessments, along with existing data on functional traits, species distribution metrics, hybrid propensity, associated biomes, realized niche description, native status and geographic origin of alien species. This resource will facilitate both fundamental and applied research and enhance our understanding of the flora’s composition and temporal changes to inform conservation efforts in the face of ongoing climate change and biodiversity loss.
Influence of diurnal phase on behavioral tests of sensorimotor performance, anxiety, learning and memory in mice
Authors: Tsao Chi-Hui , Flint Jonathan , Huang Guo-Jen 
Published: January 2022

AbstractBehavioral measurements in mice are critical tools used to evaluate the effects of interventions. Whilst mice are nocturnal animals, many studies conduct behavioral tests during the day. To better understand the effects of diurnal rhythm on mouse behaviors, we compared the results from behavioral tests conducted in the active and inactive phases. C57BL/6 mice were used in this study; we focus on sensorimotor performance, anxiety, learning and memory. Overall, our results show mice exhibit slightly higher cutaneous sensitivity, better long-term contextual memory, and a greater active avoidance escape response during the active phase. We did not observe significant differences in motor coordination, anxiety, or spatial learning and memory. Furthermore, apart from the elevated-O-maze, there was no remarkable sex effect among these tests. This study provides information on the effects of different diurnal phases on types of behavior and demonstrates the importance of the circadian cycle on learning and memory. Although we did not detect differences in anxiety and spatial learning/memory, diurnal rhythm may interact with other factors to influence these behaviors.
Protein interaction networks define the genetic architecture of preterm birth
Authors: Uzun Alper , Schuster Jessica S. , Stabila Joan , Zarate Valeria , Tollefson George A. , Agudelo Anthony , Kothiyal Prachi , Wong Wendy S. W. , Padbury James 
Published: January 2022

AbstractThe likely genetic architecture of complex diseases is that subgroups of patients share variants in genes in specific networks sufficient to express a shared phenotype. We combined high throughput sequencing with advanced bioinformatic approaches to identify such subgroups of patients with variants in shared networks. We performed targeted sequencing of patients with 2 or 3 generations of preterm birth on genes, gene sets and haplotype blocks that were highly associated with preterm birth. We analyzed the data using a multi-sample, protein–protein interaction (PPI) tool to identify significant clusters of patients associated with preterm birth. We identified shared protein interaction networks among preterm cases in two statistically significant clusters, p < 0.001. We also found two small control-dominated clusters. We replicated these data on an independent, large birth cohort. Separation testing showed significant similarity scores between the clusters from the two independent cohorts of patients. Canonical pathway analysis of the unique genes defining these clusters demonstrated enrichment in inflammatory signaling pathways, the glucocorticoid receptor, the insulin receptor, EGF and B-cell signaling, These results support a genetic architecture defined by subgroups of patients that share variants in genes in specific networks and pathways which are sufficient to give rise to the disease phenotype.
A new severity classification of lower limb secondary lymphedema based on lymphatic pathway defects in an indocyanine green fluorescent lymphography study
Authors: Shinaoka Akira , Kamiyama Kazuyo , Yamada Kiyoshi , Kimata Yoshihiro 
Published: January 2022

AbstractMost protocols for lymphatic imaging of the lower limb conventionally inject tracer materials only into the interdigital space; however, recent studies indicate that there are four independent lymphatic vessel groups (anteromedial, anterolateral, posteromedial, and posterolateral) in the lower limb. Thus, three additional injection sites are needed for lymphatic imaging of the entire lower limb. We aimed to validate a multiple injection designed protocol and demonstrate its clinical benefits. Overall, 206 lower limbs undergoing indocyanine green fluorescent lymphography with the new injection protocol were registered retrospectively. To assess the influence of predictor variables on the degree of severity, multivariable logistic regression models were used with individual known risk factors. Using a generalized linear model, the area under the curve (AUC) of the conventional clinical model, comprising known severity risk factors, was compared with that of the modified model that included defects in the posterolateral and posteromedial groups. Multivariable logistic regression models showed a significant difference for the posteromedial and posterolateral groups. The AUC of the modified model was significantly improved compared to that of the conventional clinical model. Finding defects in the posteromedial and posterolateral groups is a significant criterion for judging lymphedema severity and introducing a new lymphedema severity classification.
Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice
Authors: Dimet-Wiley Andrea , Wu Qinglong , Wiley Jerrin T. , Eswar Aditya , Neelakantan Harshini , Savidge Tor , Watowich Stan 
Published: January 2022

AbstractTreatment with a nicotinamide N-methyltransferase inhibitor (NNMTi; 5-amino-1-methylquinolinium) combined with low-fat diet (LD) promoted dramatic whole-body adiposity and weight loss in diet-induced obese (DIO) mice, rapidly normalizing these measures to age-matched lean animals, while LD switch alone was unable to restore these measures to age-matched controls in the same time frame. Since mouse microbiome profiles often highly correlate with body weight and fat composition, this study was designed to test whether the cecal microbiomes of DIO mice treated with NNMTi and LD were comparable to the microbiomes of age-matched lean counterparts and distinct from microbiomes of DIO mice maintained on a high-fat Western diet (WD) or subjected to LD switch alone. There were minimal microbiome differences between lean and obese controls, suggesting that diet composition and adiposity had limited effects. However, DIO mice switched from an obesity-promoting WD to an LD (regardless of treatment status) displayed several genera and phyla differences compared to obese and lean controls. While alpha diversity measures did not significantly differ between groups, beta diversity principal coordinates analyses suggested that mice from the same treatment group were the most similar. K-means clustering analysis of amplicon sequence variants by animal demonstrated that NNMTi-treated DIO mice switched to LD had a distinct microbiome pattern that was highlighted by decreased Erysipelatoclostridium and increased Lactobacillus relative abundances compared to vehicle counterparts; these genera are tied to body weight and metabolic regulation. Additionally, Parasutterella relative abundance, which was increased in both the vehicle- and NNMTi-treated LD-switched groups relative to the controls, significantly correlated with several adipose tissue metabolites’ abundances. Collectively, these results provide a novel foundation for future investigations.
Endothelial cells are an important source of BDNF in rat skeletal muscle
Authors: Cefis Marina , Chaney Remi , Quirié Aurore , Santini Clélia , Marie Christine , Garnier Philippe , Prigent-Tessier Anne 
Published: January 2022

AbstractBDNF (brain-derived neurotrophic factor) is present in skeletal muscle, controlling muscular metabolism, strength and regeneration processes. However, there is no consensus on BDNF cellular source. Furthermore, while endothelial tissue expresses BDNF in large amount, whether endothelial cells inside muscle expressed BDNF has never been explored. The aim of the present study was to provide a comprehensive analysis of BDNF localization in rat skeletal muscle. Cellular localization of BDNF and activated Tropomyosin-related kinase B (TrkB) receptors was studied by immunohistochemical analysis on soleus (SOL) and gastrocnemius (GAS). BDNF and activated TrkB levels were also measured in muscle homogenates using Western blot analysis and/or Elisa tests. The results revealed BDNF immunostaining in all cell types examined with a prominent staining in endothelial cells and a stronger staining in type II than type I muscular fibers. Endothelial cells but not other cells displayed easily detectable activated TrkB receptor expression. Levels of BDNF and activated TrkB receptors were higher in SOL than GAS. In conclusion, endothelial cells are an important and still unexplored source of BDNF present in skeletal muscle. Endothelial BDNF expression likely explains why oxidative muscle exhibits higher BDNF levels than glycolytic muscle despite higher the BDNF expression by type II fibers.
Rapid syndromic PCR testing in patients with respiratory tract infections reduces time to results and improves microbial yield
Authors: Serigstad S. , Markussen D. , Grewal H. M. S. , Ebbesen M. , Kommedal Ø. , Heggelund L. , van Werkhoven C. H. , Faurholt-Jepsen D. , Clark T. W. , Ritz C. , Ulvestad E. , Bjørneklett R. , Knoop S. T. ,   The CAPNOR Study Group, Bjørneklett R. , Clark T. W. , Ebbesen M. , Faurholt-Jepsen D. , Grewal H. M. S. , Heggelund L. , Knoop S. T. , Kommedal Ø. , Markussen D. , Ravn P. , Ritz C. , Serigstad S. , Ulvestad E. , van Werkhoven C. H. 
Published: January 2022

AbstractLack of rapid and comprehensive microbiological diagnosis in patients with community acquired pneumonia (CAP) hampers appropriate antimicrobial therapy. This study evaluates the real-world performance of the BioFire FilmArray Pneumonia panel plus (FAP plus) and explores the feasibility of evaluation in a randomised controlled trial. Patients presenting to hospital with suspected CAP were recruited in a prospective feasibility study. An induced sputum or an endotracheal aspirate was obtained from all participants. The FAP plus turnaround time (TAT) and microbiological yield were compared with standard diagnostic methods (SDs). 96/104 (92%) enrolled patients had a respiratory tract infection (RTI); 72 CAP and 24 other RTIs. Median TAT was shorter for the FAP plus, compared with in-house PCR (2.6 vs 24.1 h, p < 0.001) and sputum cultures (2.6 vs 57.5 h, p < 0.001). The total microbiological yield by the FAP plus was higher compared to SDs (91% (162/179) vs 55% (99/179), p < 0.0001). Haemophilus influenzae, Streptococcus pneumoniae and influenza A virus were the most frequent pathogens. In conclusion, molecular panel testing in adults with CAP was associated with a significant reduction in time to actionable results and increased microbiological yield. The impact on antibiotic use and patient outcome should be assessed in randomised controlled trials.
GPR120 prevents colorectal adenocarcinoma progression by sustaining the mucosal barrier integrity
Authors: Rubbino Federica , Garlatti Valentina , Garzarelli Valeria , Massimino Luca , Spanò Salvatore , Iadarola Paolo , Cagnone Maddalena , Giera Martin , Heijink Marieke , Guglielmetti Simone , Arena Vincenzo , Malesci Alberto , Laghi Luigi , Danese Silvio , Vetrano Stefania 
Published: January 2022

AbstractGPR120 (encoded by FFAR4 gene) is a receptor for long chain fatty acids, activated by ω-3 Polyunsaturated Fatty Acids (PUFAs), and expressed in many cell types. Its role in the context of colorectal cancer (CRC) is still puzzling with many controversial evidences. Here, we explored the involvement of epithelial GPR120 in the CRC development. Both in vitro and in vivo experiments were conducted to mimic the conditional deletion of the receptor from gut epithelium. Intestinal permeability and integrity of mucus layer were assessed by using Evans blue dye and immunofluorescence for MUC-2 protein, respectively. Microbiota composition, presence of lipid mediators and short chain fatty acids were analyzed in the stools of conditional GPR120 and wild type (WT) mice. Incidence and grade of tumors were evaluated in all groups of mice before and after colitis-associated cancer. Finally, GPR120 expression was analyzed in 9 human normal tissues, 9 adenomas, and 17 primary adenocarcinomas. Our work for the first time highlights the role of the receptor in the progression of colorectal cancer. We observed that the loss of epithelial GPR120 in the gut results into increased intestinal permeability, microbiota translocation and dysbiosis, which turns into hyperproliferation of epithelial cells, likely through the activation of β -catenin signaling. Therefore, the loss of GPR120 represents an early event of CRC, but avoid its progression as invasive cancer. these results demonstrate that the epithelial GPR120 receptor is essential to maintain the mucosal barrier integrity and to prevent CRC developing. Therefore, our data pave the way to GPR120 as an useful marker for the phenotypic characterization of CRC lesions and as new potential target for CRC prevention.
Magnetoencephalography resting-state correlates of executive and language components of verbal fluency
Authors: Oswald Victor , Zerouali Younes , Boulet-Craig Aubrée , Krajinovic Maja , Laverdière Caroline , Sinnett Daniel , Jolicoeur Pierre , Lippé Sarah , Jerbi Karim , Robaey Philippe 
Published: January 2022

AbstractVerbal fluency (VF) is a heterogeneous cognitive function that requires executive as well as language abilities. The purpose of this study was to elucidate the specificity of the resting state MEG correlates of the executive and language components. To this end, we administered a VF test, another verbal test (Vocabulary), and another executive test (Trail Making Test), and we recorded 5-min eyes-open resting-state MEG data in 28 healthy participants. We used source-reconstructed spectral power estimates to compute correlation/anticorrelation MEG clusters with the performance at each test, as well as with the advantage in performance between tests, across individuals using cluster-level statistics in the standard frequency bands. By obtaining conjunction clusters between verbal fluency scores and factor loading obtained for verbal fluency and each of the two other tests, we showed a core of slow clusters (delta to beta) localized in the right hemisphere, in adjacent parts of the premotor, pre-central and post-central cortex in the mid-lateral regions related to executive monitoring. We also found slow parietal clusters bilaterally and a cluster in the gamma 2 and 3 bands in the left inferior frontal gyrus likely associated with phonological processing involved in verbal fluency.
Assessing the impact of human mobility to predict regional excess death in Ecuador
Authors: Cuéllar Leticia , Torres Irene , Romero-Severson Ethan , Mahesh Riya , Ortega Nathaniel , Pungitore Sarah , Ke Ruian , Hengartner Nicolas 
Published: January 2022

AbstractCOVID-19 outbreaks have had high mortality in low- and middle-income countries such as Ecuador. Human mobility is an important factor influencing the spread of diseases possibly leading to a high burden of disease at the country level. Drastic control measures, such as complete lockdown, are effective epidemic controls, yet in practice one hopes that a partial shutdown would suffice. It is an open problem to determine how much mobility can be allowed while controlling an outbreak. In this paper, we use statistical models to relate human mobility to the excess death in Ecuador while controlling for demographic factors. The mobility index provided by GRANDATA, based on mobile phone users, represents the change of number of out-of-home events with respect to a benchmark date (March 2nd, 2020). The study confirms the global trend that more men are dying than expected compared to women, and that people under 30 show less deaths than expected, particularly individuals younger than 20 with a death rate reduction between 22 and 27%. The weekly median mobility time series shows a sharp decrease in human mobility immediately after a national lockdown was declared on March 17, 2020 and a progressive increase towards the pre-lockdown level within two months. Relating median mobility to excess deaths shows a lag in its effect: first, a decrease in mobility in the previous two to three weeks decreases excess death and, more novel, we found an increase of mobility variability four weeks prior increases the number of excess deaths.
Deep robust residual network for super-resolution of 2D fetal brain MRI
Authors: Song Liyao , Wang Quan , Liu Ting , Li Haiwei , Fan Jiancun , Yang Jian , Hu Bingliang 
Published: January 2022

AbstractSpatial resolution is a key factor of quantitatively evaluating the quality of magnetic resonance imagery (MRI). Super-resolution (SR) approaches can improve its spatial resolution by reconstructing high-resolution (HR) images from low-resolution (LR) ones to meet clinical and scientific requirements. To increase the quality of brain MRI, we study a robust residual-learning SR network (RRLSRN) to generate a sharp HR brain image from an LR input. Due to the Charbonnier loss can handle outliers well, and Gradient Difference Loss (GDL) can sharpen an image, we combined the Charbonnier loss and GDL to improve the robustness of the model and enhance the texture information of SR results. Two MRI datasets of adult brain, Kirby 21 and NAMIC, were used to train and verify the effectiveness of our model. To further verify the generalizability and robustness of the proposed model, we collected eight clinical fetal brain MRI 2D data for evaluation. The experimental results have shown that the proposed deep residual-learning network achieved superior performance and high efficiency over other compared methods.
Psychological factors underpinning vaccine willingness in Israel, Japan and Hungary
Authors: Goodwin Robin , Ben-Ezra Menachem , Takahashi Masahito , Luu Lan-Anh Nguyen , Borsfay Krisztina , Kovács Mónika , Hou Wai Kai , Hamama-Raz Yaira , Levin Yafit 
Published: January 2022

AbstractThe spread of SARS-CoV-2 led to rapid vaccine development. However, there remains considerable vaccine hesitancy in some countries. We investigate vaccine willingness in three nations with very different vaccine histories: Israel, Japan and Hungary. Employing an ecological-systems approach we analyse associations between health status, individual cognitions, norms, trust in government, COVID-19 myths and willingness to be vaccinated, with data from three nationally representative samples (Israel, Jan. 2021, N = 1011; Japan, Feb. 2021, N = 997; Hungary, April 2021, N = 1130). Vaccine willingness was higher in Israel (74%) than Japan (51%) or Hungary (31%). In all three countries vaccine willingness was greatest amongst who would regret not being vaccinated and respondents who trusted their government. Multi-group latent class analysis identified three groups of COVID myths, with particular concern about alteration of DNA (Israel), allergies (Hungary) and infection from the vaccine (Japan). Intervention campaigns should address such cultural myths while emphasising both individual and social benefits of vaccination.