bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contradictions in Experiences of Compulsory Sexuality and Pathways to Asexual Citizenship
Authors: Hart-Brinson Peter , Tlachac ML , Lepien Emily 
Published: December 2022

Abstract
Compulsory sexuality refers to the ways that social institutions both assume and privilege sexualities while marginalizing asexuality—the relative lack of sexual attraction. However, experiences of compulsory sexuality are not uniform. This paper documents how the institutions of compulsory sexuality can variously impede or facilitate the development of asexual citizenship, sometimes simultaneously. Data come from exploratory, semi-structured interviews with young adults who identify as asexual in the central U.S. Informants talk about their experiences with intimate relationships, religion, media, and LGBTQIA + groups in contradictory ways: each institution figures into discourses of both citizenship and alienation. We argue that there are multiple pathways to sexual citizenship for aces, which depend not only on how compulsory sexuality intersects with other structures, like race and gender, but also according to one’s experiences with the institutions of compulsory sexuality.
Understanding the Regionality and Diurnal Cycles of Precipitation in the Lake Victoria Basin during Boreal Fall
Authors: Cook Kerry H. , Vizy Edward K. 
Published: December 2022

Abstract
The diurnal cycle of rainfall in the Lake Victoria Basin of East Africa results from the super positioning of regional circulations driven by lake/land temperature differences and topography in the presence of the large-scale flow. Analysis of a triple-nested regional model simulation with a convective-permitting inner domain shows how these elements combine to produce the observed regionality of precipitation, including diurnal cycling, in boreal fall. A single diurnal rainfall peak occurs throughout the basin, but the time of maximum rainfall varies within the basin. The rainy period over the lake begins with precipitation over the northern part of the lake near 02Z (0500 LT), so it is not simply nighttime rain driven by lake/land breezes. Onset of the rainy period occurs only when low geopotential heights over the relatively warm lake cause a southward branch of the Turkana Jet to form. The formation of the jet depends on nighttime cooling over the Eastern Rift Mountains, which acts to direct the large-scale, moist flow around the topography. Topography also plays a role in the daytime rainy period over land in the Lake Victoria Basin. Moist divergence over the lake supports convergence and precipitation over the shore regions. Precipitation rates are twice the magnitude over the eastern shore compared with the western shore because daytime warming of the high elevations of the Eastern Rift Mountains allows the large-scale easterly flow to go over the mountains. This easterly flow converges with the lake/land circulation and doubles precipitation rates over the eastern shore.
Catheterization of pulmonary and carotid arteries for concurrent measurement of mean pulmonary arterial (mPAP) and systemic arterial pressure (mSAP) in PAH rats
Authors: Ahsan Fakhrul , Sarkar Tanoy , Isbatan Ayman , Moinuddin Sakib M. , Chen Jiwang , Saha Dipongkor 
Published: December 2022

Abstract

Pulmonary hypertension (PH) is a group of pulmonary vascular disorders in which mean pulmonary arterial pressure (mPAP) becomes abnormally high because of various pathological conditions, including remodeling of the pulmonary arteries, lung and heart disorders, or congenital conditions. Various animal models, including mouse and rat models, have been used to recapitulate elevated mPAP observed in PH patients. However, the measurement and recording of mPAP and mean systemic arterial pressure (mSAP) in small animals require microsurgical procedures and a sophisticated data acquisition system. In this paper, we described the surgical procedures for right heart catheterizations (RHC) to measure mPAP in rats. We also explained the catheterization of the carotid artery for simultaneous measurement of mPAP and mSAP using the PowerLab Data Acquisition system. We enumerated the surgical steps involved in exposing the jugular vein and the carotid artery for catheterizing these two blood vessels. We listed the tools used for microsurgery in rats, described the methods for preparing catheters, and illustrated the process for inserting the catheters in the pulmonary and carotid arteries. Finally, we delineated the steps involved in the calibration and setup of the PowerLab system for recording both mPAP and mSAP. This is the first protocol wherein we meticulously explained the surgical procedures for RHC in rats and the recording of mPAP and mSAP. We believe this protocol would be essential for PH research. Investigators with little training in animal handling can reproduce this microsurgical procedure for RHC in rats and measure mPAP and mSAP in rat models of PH. Further, this protocol is likely to help master RHC in rats that are performed for other conditions such as heart failure, congenital heart disease, heart valve disorders, and heart transplantation.
The Emerging Role of MRI Neurography in the Diagnosis of Chronic Inguinal Pain
Authors: Hagopian Alexa De la Fuente , Vazquez Sebastian Guadarrama-Sistos , Farhat Souha , Reddy Narainsai K. , Trakhtenbroit Michael A. , Echo Anthony 
Published: December 2022

Abstract

Chronic pain is a frequent and notable complication after inguinal hernia repair, it has been extensively studied but its management and diagnosis are still difficult. The cause of chronic pain following inguinal hernia surgery is usually multifactorial. This case series highlights the utility of MRI neurography (MRN) in evaluating the damage to inguinal nerves after a hernia repair, with surgical confirmation of the preoperative imaging findings. A retrospective review was performed on patients who underwent inguinal mesh removal and triple denervation of the groin. Inclusion criteria included MRI Neurography. All patients underwent surgical exploration of the inguinal canal for partial or complete mesh removal and triple denervation of the groin by the same senior surgeon. A total of nine patients who underwent triple denervation were included in this case series. MRN was then performed on 100% of patients. The postoperative mean VAS score adjusted for all patients was 1.6 (SD p), resulting in a 7.5 score difference compared to the preoperative VAS score (p). Since chronic groin pain can be a severely debilitating condition, diagnosis and treatment become imperative. MRN can detect direct and indirect signs of neuropathy even in the absence of a detectable compressive cause aids in management and diagnosis by finding the precise site of injury, and grading nerve injury to aid pre-operative assessment for the nerve surgeon. Thus, it is a valuable diagnostic tool to help with the diagnosis of nerve injuries in the setting of post-inguinal hernia groin pain.
Engineering extracellular vesicles with macrophage membranes fusion ameliorated psoriatic skin inflammation in imiquimod‐treated mice
Authors: Wang Zeng , Qin Zhizhen , Wang Jiadie , Xu Xinqi , Zhang Mengxin , Liang Yuyue , Huang Yukun , Yu Zengyang , Gong Yu , Zhou Luxian , Qiu Yiran , Ma Minglu , Li Dan , Li Bin 
Published: December 2022

Abstract
Backgrounds: Psoriasis is an autoimmune skin disease that is difficult to cure and easy to relapse after drug withdrawal. Innate macrophage polarization reprogramming has a considerable impact on psoriasis prognosis. Herein, we introduce a method to ameliorate inflammatory responses based on macrophage membrane-engineered extracellular vesicles.
Results: Engineered extracellular vesicles inherited the high stability of M2 macrophage membrane and retained the macrophage reprogramming potential of Annexin A1 overexpressing T cell-derived exosomes. In the psoriasis-like skin mouse model, engineered extracellular vesicles successfully ameliorated inflammatory responses in the skin and spleen with high biosafety.
Conclusions: Our findings indicated that the M2 macrophage-like fusogenic extracellular vesicle-delivery platform had high inflammation-regulating ability and provided new insights and potential strategies for immunotherapy.
Structural and molecular insight into the disintegration of BRPs released by massive wasp stings using serratiopeptidase
Authors: Dhiman Ankita , Purohit Rituraj 
Published: December 2022

Abstract
Objective and design: Serratiopeptidase a multifaceted therapeutic enzyme renowned for its anti-inflammatory, analgesic, anti-biofilm, fibrinolytic and anti-edemic properties. It is vital to uncover more about the assets of such efficacious enzyme in order to facilitates their contribution in all health-related issues, notably inflammatory ailments. The current study sought to determine whether serratiopeptidase would disintegrate bradykinin related peptides (BRPs) from wasp venom in the same manner as it does with human bradykinin. Methods: To accomplish this objective, we used molecular modeling, docking, MD simulation, MMG/PBSA along with the SMD simulations. Results: We docked selected BRPs on to the binding pocket of wild and previously identified mutant (N412D) of serratiopeptidase. Based on their docked scores, top two BRPs were selected and their conformational behavior was analyzed employing molecular dynamics studies. Additionally, thermodynamics end-state energy analysis reported that both the complexes exhibited higher stability and analogous ∆G values when compared to the reference complex. Further, to understand the unbinding mechanism, we condemned external pulling force on both peptides and observed that BRP-7 peptide was tightly anchored and laid out the highest pulling force to get detach from the active pocket of serratiopeptidase. Conclusion: The current study endorses up the current findings and paves the way for serratiopeptidase to be used as an anti-angioedemic as well as fixed dose combination in hypotensive drugs.
Controlled Fabrication of RuOxSey Composites for Enhanced Acidic Oxygen Evolution
Authors: Du Peng , Lin Chaoliang , He Xian , Zheng Zhichuan , Xie Xinyu , Huang Kai , Lei Ming , Tang Haolin 
Published: December 2022

Abstract

Developing relatively cost-effective and high-performance Ru-based composites as electrocatalysts for acidic oxygen evolution has become the critical step toward emerging renewable energy conversion, in which exploring controllable synthetic strategies and investigating the intrinsic nature by non-metallic interfacial modulation is highly desirable. In this work, a facile tandem process is proposed by ball-milling and thermal annealing for the scalable synthesis of RuOxSey nanocomposites with in-situ formed crystalline RuSe2 and metallic Ru heterostructures on RuO2 substrate. The optimized RuOxSey-800 sample has been proved to an excellent OER activity and long-term stability in an acidic electrolyte due to its suitable microscopic morphology and phase composition, along with a relatively smaller Tafel slope of 45.4 mV dec− 1 compared with commercial RuO2 and most similar catalysts reported in the literature. The outstanding acidic OER performance can be ascribed to the enhanced electron transfer and more active site exposure by controllable selenization. This work paves a novel way for the design and large-scale production of various non-metallic modified composite catalysts for extensive applications of energy conversion and storage.
NAM-NAD+-ADO metabolic reprogramming is a key factor for DNMT3A mutation to promote leukemia development through regulating cell cycle and immune microenvironment
Authors: Wang Yueying , Yang Xuejiao , Wang Xiao , Yang Ying , Li Zhiyang , Chen Yunshuo , Shang Siqi 
Published: December 2022

AbstractDNMT3A-R882H mutation is a frequent epigenetic mutation in acute myeloid leukemia (AML)-M4 and M5, participating in the regulation of cell growth and differentiation by blocking the binding of transcription factor complexes to DNA. However, effective approaches that directly target this inactivating mutation for therapy remain lacking. For the first time, we show that the DNMT3A-R882H mutation can switch leukemia cells to undergo nicotinamide (NAM) and nicotinamide adenine dinucleotide (NAD+) metabolism and influence the formation of cyclin-CDK complexes by affecting the deacetylation function of SIRT6. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) can reduce the NAD + required for SIRT6 function, degrade CDKN1A/CDKN1B, and effectively induce cell cycle arrest and apoptosis. The NAMPT inhibitor FK866 can optimize the immune-infiltrating microenvironment by reducing the production of the downstream metabolite adenosine (ADO). Animal experiments have shown that NAMPT inhibition or knockdown can significantly inhibit tumor cell growth, reduce spleen infiltration of tumor cells, and prolong mouse lifespan. Our findings provide a potential direction for a targeted therapy at the metabolic level in AML with DNMT3A mutations.
Global mapping reveals more intensively exploited inland than coastal wetlands
Authors: Feng Lian , Xu Yang , Fang Hongwei , Song Xiao-Peng , Gieseke Fabian , Kariryaa Ankit , Oehmcke Stefan , Gibson Luke , Jiang Xiating , Lin Ruimin , Xu Wang , Zheng Chunmiao , Brandt Martin , Fensholt Rasmus 
Published: December 2022

Abstract
Losses of coastal wetlands have been observed worldwide, but the extent to which inland wetlands have been exploited by humans is currently unknown on a global scale. Here, we map the distribution of land reclamation over global inland and coastal wetlands using high-resolution satellite observations. Results show that the total area of reclaimed wetlands was 132,886 km2 globally, with about 2/3 contributed from inland wetlands. From 1984 to 2020, the net gain of reclaimed inland wetlands (26,385 km2) was 1.6 times that of coastal wetlands (16,371 km2), due primarily to reduced coastal exploitation in the last decade. We identified significant contributions of wetland reclamation to fishery production, and further revealed greater reclamation rates within than outside protected areas. Our study highlights that the more intensively reclaimed inland wetlands should be highly prioritized, and our global synthesis provides a critical benchmark for assessing potential consequences of wetland exploitation and for future restoration efforts.
On Demand Deployment of Edge Cloud Infrastructures for Federated Learning
Authors: Huedo Eduardo , Moreno-Vozmediano Rafael , Montero Rubén S. , Llorente Ignacio M. 
Published: December 2022

Abstract
Federated learning on the edge allows the use of more powerful servers and more complex training models. This paper presents the deployment of a real federated learning framework on top of a real geo-distributed edge computing infrastructure, based on a commercial edge provider, using the OpenNebula cloud platform. Results show the feasibility, performance and cost efficiency of the solution.
A prediction nomogram based on machine learning methods for acute respiratory distress syndrome in patients with acute pancreatitis: A retrospective study
Authors: Hu Hongjie , Wang Yuxin , Song Yaqin , Wu Shuhui , Li Dayong , Jing Liang , Qin Lei , Xia Zhaohui , Zhu Wei 
Published: December 2022

Abstract
To construct a predictive nomograph for acute pancreatitis (AP) complicated with acute respiratory distress syndrome (ARDS) admitted to the intensive care unit (ICU) using machine learning methods. This study was designed as a retrospective investigation of data from patients enrolled with AP. These patients were divided into a training cohort and a testing cohort. Machine learning methods were used to select independent predictive factors and to establish a nomogram model, and the feasibility of the nomogram model was evaluated by the test set. The area under the receiver operating characteristic curve (AUC), calibration curve, and decision curve analysis (DCA) were used to assess the discriminatory ability, precision, and clinical applicability of the model. A total of 427 patients were included, 344 of whom were in the training cohort and 83 in the testing cohort. The support vector machine (SVM) model showed the best performance among the six machine-learning models. Age, sex, the sequential organ failure score (SOFA), C-reactive protein (CRP), platelet count (PLT), total serum bilirubin (TBIL), and direct bilirubin (DBIL) levels were the best predictors for patients with AP presenting ARDS through the SVM learning model. These seven variables were incorporated to construct a nomogram. The C-index of the model was 0.7977 in the training cohort and 0.8484 in the testing cohort. The calibration curve for predictive probability showed that the nomogram-based predictions were in good agreement with the actual observations. The DCA plot demonstrated a good net benefit for this model, and external validation confirmed its reliability. The prediction nomogram constructed based on the SVM model in this study can effectively predict the probability of AP complicated by ARDS.
Molecular diagnosis of urogenital schistosomiasis in pre-school children, school-aged children and women of reproductive age at community level in central Senegal
Authors: Sow Doudou , Sylla Khadime , Dieng Ndeye Marème , Senghor Bruno , Gaye Papa Mouhamadou , Fall Cheikh B. , Goumballa Ndiaw , Diallo Aldiouma , Ndiaye Jean Louis A. , Parola Philippe , Sokhna Cheikh , Doucouré Souleymane , Faye Babacar 
Published: December 2022

Abstract
Background: Urogenital schistosomiasis is a major public health concerns in Sub-Saharan Africa. In Senegal, the disease is endemic in all the regions of the country. Recently, WHO strongly recommend including the pre-school children and the women of reproductive age during the mass drug administration campaign. So, it is important to describe the burden of the disease in these group at risk using innovative diagnosis tools. This study aimed to assess the use of real time PCR in the detection of schistosomiasis cases at community level in a seasonal transmission area.
Materials and methods: A cross sectional survey was carried out in Niakhar located in the centre of Senegal. Pre-schoolchildren, school-aged children and female adolescents and adults were invited to participate in the study in April 2018. Urine samples were collected and examined using hemastix reagents strips, filtration technique and real-time PCR. Schistosoma haematobium was detected identified by targeting the Dra1 gene. The prevalence of urogenital schistosomiasis was determined for each group and the performance of the real-time PCR was compared with the conventional techniques.
Results: A total of 428 participants were enrolled in this study including 87 (20.3%) pre-school children (1-5 years), 262 (61.2%) school-aged children between (5-14 years), 17 (3.9%) adolescents (15-17 years) and 62 (14.4%) female adults. The comparison of the diagnostic techniques has shown that the prevalence of urogenital schistosomiasis is more important using molecular technique (34.6%) compared to the microscopy (20.3%). The percentage rate of hematuria using the hemastix was 23.1%. School-aged children between 5 and 14 years old were the most affected with 29.0% and 43.1% under microscopy and RT-PCR respectively. In female participants, the microscopic prevalence decreases with the age, from 21.4% in school-aged children to 17.6% in adolescents and 9.7% in adults. There was a good correlation between the number of eggs per 10 ml and the cycle threshold range.
Conclusion: These results show the importance of the use of molecular tools in the surveillance of schistosomiasis particularly in pre-school children and women of reproductive age.
Revealing Corynebacterium glutamicum proteoforms through top- down proteomics
Authors: Melo Reynaldo Magalhães , de Souza Jaques Miranda Ferreira , Williams Thomas Christopher Rhys , Fontes Wagner , de Sousa Marcelo Valle , Ricart Carlos André Ornelas , Vale Luis Henrique Ferreira do 
Published: December 2022

Abstract
Corynebacterium glutamicum is a bacterium widely employed in the industrial production of amino acids as well as a broad range of other biotechnological products. The present study describes the characterization of C. glutamicum proteoforms, and their post-translational modifications (PTMs) employing top-down proteomics. Despite previous evidence of PTMs having roles in the regulation of C. glutamicum metabolism, this is the first top-down proteome analysis of this organism. We identified 1125 proteoforms from 273 proteins, with 60% of proteins presenting at least one mass shift, suggesting the presence of PTMs, including several acetylated, oxidized and formylated proteoforms. Furthermore, proteins relevant to amino acid production, protein secretion, and oxidative stress were identified with mass shifts suggesting the presence of uncharacterized PTMs and proteoforms that may affect biotechnologically relevant processes in this industrial workhorse. For instance, the membrane proteins mepB and SecG were identified as a cleaved and a formylated proteoform, respectively. While in the central metabolism, OdhI was identified as two proteoforms with potential biological relevance: a cleaved proteoform and a proteoform with PTMs corresponding to a 70 Da mass shift.
Atenolol oxidation by Zeolite X encapsulated Cu (II)-L complex (L = a Schiff base ligand)
Authors: Hasani Fatmeh , Tabatabaee Masoumeh , Sharif Mahboubeh A. , Mahmoodi Mhboubeh 
Published: December 2022

Abstract
In this research zeolite‑X encapsulated M(II)-L complex (M = Co, Cu and L = 6,6'-((1E,1'E)-(ethane-1,2-diylbis(azanylylidene))bis(methanylylidene))bis(2-methoxyphenol)) was selected as an efficient heterogeneous catalytic for the oxidation of atenolol as beta-blocker. The reaction is monitored spectrophotometrically by measuring the absorbance of oxidation product at λ = 350 nm and thin layer chromatography (TLC). Some parameters including concentration of catalyst, concentration of H2O2 and reaction time were investigated and optimized. Approximately all atenolol has been oxidized after 120 min in the presences of M-L-X as catalyst, H2O2 as oxidant and optimizes conditions. Study of recovering and reusing of catalyst show the regenerated catalyst can be used for several times with negligible activity losses compared to first time.