bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of the Catalytic Properties of Immobilized Recombinant Lipase from Thermomyces lanuginosus in the Reaction of Esterification by the Selection of an Adsorbent
Authors: Kovalenko G. A. , Perminova L. V. , Beklemishev A. B. , Pykhtina M. B. , Holyavka M. G. , Buchelnikova V. A. , Artyukhov V. G. 
Published: September 2022

Abstract
Biocatalysts with lipase activity (BLAs) were prepared by adsorptive immobilization of recombinant lipase (rPichia/lip) from thermophilic microscopic fungi Thermomyces lanuginosus produced by a genetically engineered strain of methylotrophic yeast Komagataella phafii (Pichia pastoris). Supports with different physicochemical properties were used as adsorbents: mesoporous hydrophilic silica (SiO2) and macroporous hydrophobic carbon aerogel (MCA). The enzymatic activity, substrate specificity and operational stability of BLAs were studied in the esterification of saturated fatty acids with aliphatic alcohols differing in the number of carbon atoms in the molecule from 2 to 18. Matrices of relative activities were compiled for more than 60 pairs of substrates, an acid and an alcohol, by comparing the reaction rates of the esterification under identical conditions, which allowed us to reveal differences in the specificity of adsorbed lipase depending on the chemical nature of the support. It was found that for both types of biocatalysts, rPichia/lip on SiO2 (PLSi) and rPichia/lip on MCA (PLC), the maximum reaction rate was observed under esterification of heptanoic acid (C7) with butyl alcohol (C4). Under the same conditions of the synthesis of esters (20 ± 2°C, 1 bar, a mixture of hexane and diethyl ether as an organic solvent), including the synthesis of butylheptanoate, rPichia/lip adsorbed on silica showed an order of magnitude lower activity than lipase adsorbed on carbon aerogel. The catalytic constants, equal to 3.7 s–1 and 1.1 × 102 s–1, respectively, differed by 30 times. It was found that esters of short chain fatty acids C4–C7 and ethyl alcohol C2 were synthesized 2–3 times faster using the hydrophobic PLC type than using the hydrophilic PLSi type of BLAs. At the same time, esters of high-molecular-weight acids С9, C10, С18 and alcohols С8–С16 with pronounced hydrophobicity were synthesized 1.5–2 times faster using of PLSi type BLAs. The operational stability of the biocatalysts was quite high: the prepared BLAs retained 82–99% of their initial activity after more than 30 reaction cycles, while the duration of each cycle to reach an acid conversion above 85% was several hours (4–6 h).
Enzymatically Synthesized Polyaniline Doped with Copper Ions: Physico-Chemical and Antimicrobial Properties of the Product
Authors: Vasil’eva I. S. , Shumakovich G. P. , Morozova O. V. , Yaropolov A. I. 
Published: September 2022

AbstractEnzymatic synthesis of the polyaniline (PANI)/sodium polystyrenesulfonate (PSS) interpolyelectrolyte complex, in which PANI is doped with Cu(II) ions, has been developed. The biocatalyst for aniline (ANI) polymerization was the fungal laccaseTrametes hirsutaand the oxidizing agent was atmospheric oxygen. The resulting PANI-Cu/PSS complex was studied by UV–visible and FTIR-ATR spectroscopy, and X-ray fluorescence analysis. The copper content in PANI‑Cu/PSS was ~8 wt %. The minimum inhibitory concentration (MIC) of the PANI-Cu/PSS complex against gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria was 2.65 and 0.66 mg/mL, respectively.
Politics and Strategy of the United States, the Russian Federation, and China in the Middle East
Authors: Batyuk V. I. , Morozov Yu. V. 
Published: September 2022

Abstract
The “triangle” of America–China–Russia formed during the Cold War of 1945–1991 still has a huge impact on modern world politics. And the balance of power within this “triangle” has been determined and will be determined by the ability of Washington, Moscow, and Beijing to promote their interests in various regions of the planet. Of course, one of the most important of these regions was, is, and will be the Middle East, where the economic, geostrategic, and ideological interests of the leading centers of power in the modern world collide.
Countermeasures for Hybrid Threats: The Experience of the European Union and Its Member States
Authors: Bazarkina D. Yu. 
Published: September 2022

Abstract
The methods and tools used by the European Union to counter hybrid threats are identified: from the fight against terrorism to measures aimed at combating economic competitors and political opponents (mainly, to squeeze Russia and China out of European markets). It is concluded that it is not by chance that neither EU institutions nor the research community have worked out a comprehensive definition of operations to combat hybrid threats. A broad understanding of hybrid threats as practically any (depending on the political situation) actions of the opponent serves to justify the application of any counteraction tool. In the fight against global threats such as terrorism, cybercrime, and the spread of false medical data, the EU takes a systemic approach, which makes it possible to assess the level and degree of the convergence of threats to critical infrastructure and the infosphere, as well as the possibilities of counteraction. At the same time, attempts to use economic, legislative, political, and informational tools to achieve one-sided economic, political, and military advantages do not reduce the degree of tension in the EU’s relations with Russia, China, and some other countries, only increasing the number and strength of hybrid threats. This reduces the EU’s ability to achieve strategic autonomy.
Coronary atherosclerotic burden assessed by SYNTAX scores and outcomes in surgical, percutaneous or medical strategies: a retrospective cohort study
Authors: Scudeler Thiago Luis , Farkouh Michael E , Hueb Whady , Rezende Paulo C , Campolina Alessandro G , Martins Eduardo Bello , Godoy Lucas C , Soares Paulo Rogério , Ramires Jose A F , Kalil Filho Roberto 
Published: September 2022

IntroductionCoronary atherosclerotic burden and SYNTAX Score (SS) are predictors of cardiovascular events.ObjectivesTo investigate the value of SYNTAX scores (SS, SYNTAX Score II (SSII) and residual SYNTAX Score (rSS)) for predicting cardiovascular events in patients with coronary artery disease (CAD).DesignRetrospective cohort study.SettingSingle tertiary centre.ParticipantsMedicine, Angioplasty or Surgery Study database patients with stable multivessel CAD and preserved ejection fraction.InterventionsPatients with CAD undergoing coronary artery bypass graft (CABG), percutaneous coronary intervention (PCI) or medical treatment (MT) alone from January 2002 to December 2015.Primary and secondary outcomesPrimary: 5-year all-cause mortality. Secondary: composite of all-cause death, myocardial infarction, stroke and subsequent coronary revascularisation at 5 years.ResultsA total of 1719 patients underwent PCI (n=573), CABG (n=572) or MT (n=574) alone. The SS was not considered an independent predictor of 5-year mortality in the PCI (low, intermediate and high SS at 6.5%, 6.8% and 4.3%, respectively, p=0.745), CABG (low, intermediate and high SS at 5.7%, 8.0% and 12.1%, respectively, p=0.194) and MT (low, intermediate and high SS at 6.8%, 6.9% and 6.5%, respectively, p=0.993) cohorts. The SSII (low, intermediate and high SSII at 3.6% vs 7.9% vs 10.5%, respectively, p<0.001) was associated with a higher mortality risk in the overall population. Within each treatment strategy, SSII was associated with a significant 5-year mortality rate, especially in CABG patients with higher SSII (low, intermediate and high SSII at 1.8%, 9.7% and 10.0%, respectively, p=0.004) and in MT patients with high SSII (low, intermediate and high SSII at 5.0%, 4.7% and 10.8%, respectively, p=0.031). SSII demonstrated a better predictive accuracy for mortality compared with SS and rSS (c-index=0.62).ConclusionsCoronary atherosclerotic burden alone was not associated with significantly increased risk of all-cause mortality. The SSII better discriminates the risk of death.Trial registration numberISRCTN66068876.
’It stayed there, front and centre’: perspectives on community pharmacy’s contribution to front-line healthcare services during the COVID-19 pandemic in Northern Ireland
Authors: Patterson Susan M , Cadogan Cathal A , Barry Heather E , Hughes Carmel M 
Published: September 2022

ObjectivesTo explore community pharmacists and key stakeholders’ perspectives and reflections on the community pharmacy workforce’s preparedness for, and response to, COVID-19, including lessons for future public health crises.Design, setting and participantsQualitative study using semistructured interviews (via telephone or online videoconferencing platform), with community pharmacists and a range of key stakeholders (representing other health professions, professional/governing organisations concerned with community pharmacy and patient advocacy groups) from across Northern Ireland. Data were analysed using thematic analysis and constant comparison.ResultsThirty interviews were conducted with community pharmacists (n=15) and key stakeholders (n=15). Four themes were identified: (1) adaptation and adjustment (reflecting how community responded quickly to the need to maintain services and adjusted and adapted services accordingly); (2) the primary point of contact (the continuing accessibility of community pharmacy when other services were not available and role as a communication hub, particularly in relation to information for patients and maintaining contact with other healthcare professionals); (3) lessons learnt (the flexibility of community pharmacy, the lack of infrastructure, especially in relation to information technology, and the need to build on the pandemic experience to develop practice); and (4) planning for the future (better infrastructure which reinforced concerns about poor technology, coordination of primary care services and preparing for the next public health crisis). There was a general view that community pharmacy needed to build on what had been learnt to advance the role of the profession.ConclusionsThe strengths of community pharmacy and its contribution to healthcare services in the COVID-19 pandemic were noted by community pharmacists and acknowledged by key stakeholders. The findings from this study should inform the policy debate on community pharmacy and its contribution to the public health agenda.
Academic-practice partnerships in evidence-based nursing practice: a scoping review protocol
Authors: Halili Xirongguli , Välimäki Maritta , Li Zeen , Tang Siyuan , Wang Honghong , Chen Qirong 
Published: September 2022

IntroductionAcademic-practice partnerships are a promising strategy that could strengthen the promotion and innovation of evidence-based nursing practice (EBNP). However, there is little evidence of how academic and clinical institutions and individuals should collaborate in each process of EBNP and the factors that influence academic-practice partnerships in EBNP. There is a pressing need to explore the extent of the literature on academic-practice partnerships in EBNP, as well as to classify, compare and summarise the results or opinions obtained from various types of literature to identify both existing knowledge and gaps in the research.Methods and analysisThe scoping review will be conducted following the methodological guidelines provided by the JBI. The scoping review will be reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews checklist. Seven databases, including the Cochrane Library, PubMed, Web of Science, CINAHL, EMBASE, SCOPUS, Educational Resource Information Center and two Chinese databases (ie, CNKI and WANFANG DATA), will be searched. The grey literature will also be searched using the American Association of Colleges of Nursing, American Nurses Association, Open Grey, Grey Literature Report and the official website of JBI. The literature screening and data extraction will be conducted independently by two researchers. A third researcher will be involved when a consensus is needed.Ethics and disseminationEthics approval is not required. The findings of the scoping review will be disseminated in a conference and a peer-reviewed journal.
Clinical detection of ‘cold stress’ is overlooked: an online survey of healthcare workers to explore the gap in neonatal thermal care in low-resource settings
Authors: Kyokan Michiko , Jirapaet Veena , Rosa-Mangeret Flavia , Pisoni Giorgia Brambilla , Pfister Riccardo E 
Published: September 2022

BackgroundNeonatal hypothermia has been widely regarded as a major contributory factor to neonatal mortality and morbidity in low-resource settings. The high prevalence of potentially preventable hypothermia today urges an investigation into why neonates still become hypothermic despite awareness of the problem and established thermal care guidelines. This study aimed to explore the gaps in knowledge and practices of neonatal thermal care among healthcare workers in low-resource settings.MethodsA cross-sectional, questionnaire-based survey was performed online among healthcare workers in low-resource settings. We applied a purposive and snowballing sampling method to recruit participants through a two-round international online survey. Questionnaires were developed using themes of neonatal thermal care extracted from existing neonatal care guidelines.Results55 neonatal care professionals participated in the first-round survey and 33 in the second. Almost all participants (n=44–54/55) acknowledged the importance of the WHO’s warm chain to keep a neonate warm. However, fewer participants (n=34–46/55) responded to practice them. When asked about cold stress, defined as a condition in which neonates are below optimum environmental temperature and using more oxygen and energy while maintaining normal body temperature, 15 out of 55 participants answered that checking extremity temperatures by hand touch was useless. Some participants reported concern about the extremity temperature’s inaccuracy compared with core temperature. Opinions and preferences for rewarming methods differed among participants, and so did the availability of warming equipment at their institutions.ConclusionAn inadequate understanding of cold stress underestimates the potential benefits of extremity temperatures and leads to missed opportunities for the timely prevention of hypothermia. The current thermal care guidelines fail to highlight the importance of monitoring cold stress and intervening before hypothermia occurs. Therefore, we urge introducing the concept of cold stress in any neonatal thermal care guidelines.
Endovascular therapy in acute anterior circulation large vessel occlusive patients with a large infarct core (ANGEL-ASPECT): protocol of a multicentre randomised trial
Authors: Huo Xiaochuan , Ma Gaoting , Zhang Xuelei , Pan Yuesong , Tong Xu , Sun Dapeng , Liu Liping , Wang Yilong , Liebeskind David S , Wang Yongjun , Pereira Vitor Mendes , Ren Zeguang , Miao Zhongrong 
Published: September 2022

BackgroundThe benefit of stroke thrombectomy for large infarct core still lacks robust randomised controlled studies.AimTo demonstrate the design of a clinical trial on endovascular therapy for acute anterior circulation large vessel occlusion (LVO) patients with large infarct core volume.DesignANGEL-ASPECT is a multicentre, prospective, randomised, open-label, blinded End-point trial to evaluate whether best medical management (BMM) combined with endovascular therapy improves neurological functional outcomes as compared with BMM alone in acute LVO patients with Alberta Stroke Program Early CT Score (ASPECTS) of 3–5 on non-contrast CT or infarct core volume range of 70–100 mL (defined as rCBF <30% on CT perfusion or ADC <620 on MRI) up to 24 hours from symptom onset or last seen well.Study outcomesThe primary efficacy outcome is 90 (±7) days modified Rankin Scale. Symptomatic intracranial haemorrhage within 48 hours from randomisation is the primary safety outcome.DiscussionThe ANGEL-ASPECT trial will screen patients with large infarct core (ASPECTS 3–5 or 70–100 mL) through image evaluation criteria within 24 hours and explore the efficacy and safety of endovascular therapy compared with BMM.
Event-shape engineering analysis of D meson in ultrarelativistic heavy-ion collisions
Authors: Sambataro Maria Lucia , Sun Yifeng , Minissale Vincenzo , Plumari Salvatore , Greco Vincenzo 
Published: September 2022

AbstractWe describe the propagation of charm quarks in the quark-gluon plasma (QGP) by means of an event-by-event transport approach. In our calculations the non-perturbative interaction between heavy quarks and light partons has been taken into account through a quasi-particle approach with thermal light quark masses tuned to reproduce lQCD thermodynamics. We found that the flow observables $$v_2$$

v
2

and $$v_3$$

v
3

of D mesons are comparable with the experimental measurements for Pb + Pb collisions at 5.02 TeV in different ranges of centrality selections. The results are analyzed with event-shape engineering technique. The comparison of the anisotropic flow coefficients $$v_n$$

v
n

with experimental data show a quite good agreement with experimental data for different flow vector $$q_2$$

q
2

selections, which confirms the strong coupling between charm quarks and light quarks in the QCD matter. Furthermore, we present here a novel study of the event-by-event correlations between flow harmonics of D mesons and soft hadrons at LHC energy with the event-shape engineering technique that can put further constraints on heavy quark transport coefficients toward a solid comparison between the phenomenological determination and the lattice QCD calculations.
Some identities involving generalized (α,β)-derivations in prime and semiprime rings
Authors: Bera Manami , Dhara Basudeb , Kar Sukhendu 
Published: September 2022

Let [Formula: see text] be a prime or semiprime ring, [Formula: see text] a nonzero left-sided ideal of [Formula: see text] and [Formula: see text] be two automorphisms of [Formula: see text]. Let [Formula: see text] and [Formula: see text] be two generalized [Formula: see text]-derivations of [Formula: see text] associated with [Formula: see text]-derivations [Formula: see text] and [Formula: see text], respectively. The purpose of this paper is to investigate the following identities: (1) [Formula: see text]; (2) [Formula: see text]; (3) [Formula: see text]; (4) [Formula: see text]; (5) [Formula: see text]; (6) [Formula: see text]; (7) [Formula: see text]; (8) [Formula: see text]; (9) [Formula: see text]; (10) [Formula: see text]; (11) [Formula: see text]; (12) [Formula: see text], for all [Formula: see text].
Mortality during In-Hospital Treatment for Head and Neck Cancer in Germany: A Diagnosis-Related Group-Based Nationwide Analysis, 2005–2018
Authors: Hermanns Isabel , Kouka Mussab , Schlattmann Peter , Guntinas-Lichius Orlando 
Published: September 2022

Background. Data on in-hospital MR (IHMR) of head and neck cancer (HNC) are sparse. Methods. IHMR was determined in Germany between 2005 and 2018 using nationwide population-based diagnosis-related group (DRG) data of 1,090,596 HNC. Results. The overall average IHMR was

0.04
±
0.02

. IHMR increased with older age to

0.04
±
0.01

for patients of 65-79 years of age (relative risk [RR] in relation to patients of 35-49 years of

age
=
1.767

;

95
%
confidence

interval

CI

=
1.040

to3.001) to a maximum of

0.07
±
0.01

for patients of 80 years and older (

RR
=
2.826

;

CI
=
1.663

to 4.803). IHMR was the highest when no HNC-specific treatment, i.e., best supportive and palliative care, was applied (

0.11
±
0.01

; RR in relation to

tumor

biopsy

surgery
=
7.241

;

CI
=
3.447

to 5.211). IHMR was not different between surgery, radiotherapy, or chemotherapy/biologicals. Conclusions. IHMR did not change over time. Efforts are needed to decrease the IHMR for HNC.
Satisfactions of Customers by Using Online Food Application Services During Covid-19 Pandemic
Authors: Rosa Honeylyn R. Dela , Separa Lenis Aislinn C. 
Published: September 2022

Food businesses benefit from an online food ordering and delivery framework. The purpose of this study is to identify the factors that influence overall consumer satisfaction with online food delivery providers. The demographic information of the respondents was discussed in this study. Furthermore, this study also revealed a significant difference between respondents' perceptions of e-services and the variables listed in the study's objectives.
The researchers used a quantitative approach to identify the factors that influence consumers' overall satisfaction with online food delivery services. To collect information from the respondents, the researchers used a convenience and snowball sampling technique. Descriptive statistics were used to depict the respondent profile. The Mann-Whitney U and Wilcoxon tests were used to determine the difference in e-satisfaction between female and male respondents, while the Kruskal-Wallis test was used to determine the difference in e-satisfaction of respondents when grouped by age.
The Spearman rho was used to see if there was a link between personal aspect, E-Service Quality, and Perceived Food Quality and e-satisfaction. The study found a significant relationship between personal aspect, E-Service Quality, and Perceived Food Quality and e-satisfaction.
In terms of gender, most of the respondents are male, with 51.3%, and for the age of respondents, 29 to 40 years old. In terms of employment status, employed with a total of 162 and 61.60% has participated in the study. income salary, 50,000 and above are the highest, with a frequency of 64 and a percentage of 27.71%.
LncRNA PSR Regulates Vascular Remodeling Through Encoding a Novel Protein Arteridin
Authors: Yu Junyi , Wang Wei , Yang Jining , Zhang Ye , Gong Xue , Luo Hao , Cao Nian , Xu Zaicheng , Tian Miao , Yang Peili , Mei Qiao , Chen Zhi , Li Zhuxin , Li Chuanwei , Duan Xudong , Lyu Qing Rex , Gao Chen , Zhang Bing , Wang Yibin , Wu Gengze , Zeng Chunyu 
Published: September 2022

Rationale:
Vascular smooth muscle cells (VSMCs) phenotype switch from contractile to proliferative phenotype is a pathological hallmark in various cardiovascular diseases. Recently, a subset of long noncoding RNAs was identified to produce functional polypeptides. However, the functional impact and regulatory mechanisms of long noncoding RNAs in VSMCs phenotype switching remain to be fully elucidated.

Objectives:
To illustrate the biological function and mechanism of a VSMC-enriched long noncoding RNA and its encoded peptide in VSMC phenotype switching and vascular remodeling.

Results:

We identified a VSMC-enriched transcript encoded by a previously uncharacterized gene, which we called phenotype switching regulator (
PSR
), which was markedly upregulated during vascular remodeling. Although PSR was annotated as a long noncoding RNA, we demonstrated that the lncPSR (PSR transcript) also encoded a protein, which we named arteridin. In VSMCs, both arteridin and lncPSR were necessary and sufficient to induce phenotype switching. Mechanistically, arteridin and lncPSR regulate downstream genes by directly interacting with a transcription factor YBX1 (Y-box binding protein 1) and modulating its nuclear translocation and chromatin targeting. Intriguingly, the PSR transcription was also robustly induced by arteridin. More importantly, the loss of PSR gene or arteridin protein significantly attenuated the vascular remodeling induced by carotid arterial injury. In addition, VSMC-specific inhibition of lncPSR using adeno-associated virus attenuated Ang II (angiotensin II)–induced hypertensive vascular remodeling.

Conclusions:
PSR is a VSMC-enriched gene, and its transcript IncPSR and encoded protein (arteridin) coordinately regulate transcriptional reprogramming through a shared interacting partner, YBX1. This is a previously uncharacterized regulatory circuit in VSMC phenotype switching during vascular remodeling, with lncPSR/arteridin as potential therapeutic targets for the treatment of VSMC phenotype switching–related vascular remodeling.