bluuebunny/crossref_metadata_2025_split huggingface dataset:
GiantScope: A simulation microscopy for middle school biological experiment education
Authors: Zhang Haoyang , Zhang Mingmin , Pan Zhigeng , Cao Mingliang , Li Yongheng , Zhong Yong , Chen Ganglin , Liu Xin 
Published: June 2022

AbstractWe present a simulation microscope device called GiantScope, which combines virtual microscope, cloud computing, and embedded technologies. Users can complete most of the microscope‐based experiments in biology courses by operating our device, while learning the operating skills of microscopes at the same time. Our device supports most of the operation functions of optical microscopes, including quasifocus screw adjustment, slide movement recognition and so on, and also has auxiliary enhancement functions including manual measurement, annotation and so on. In addition, we have built a cloud‐based digital slide database, which enables users to select experimental observations through digital slides, including static cell specimens or dynamic cell activities. After user study, we found that using GiantScope for biological experiments has better learning efficiency and user experience than traditional microscopes.
STAT Protein Thermal Shift Assays to Monitor Protein‐Inhibitor Interactions
Authors: Iliev Petar , Hanke Danielle , Page Brent D. G. 
Published: June 2022

AbstractSTAT3 protein is a sought‐after drug target as it plays a key role in the progression of cancer. Many STAT3 inhibitors (STAT3i) have been reported, but accumulating evidence suggests many of these act as off‐target/indirect inhibitors of STAT signaling. Herein, we describe the STAT protein thermal shift assay (PTSA) as a novel target engagement tool, which we used to test the binding of known STAT3i to STAT3 and STAT1. This revealed STATTIC, BP‐1‐102, and Cpd188 destabilized both STATs and produced unique migratory patterns on SDS‐PAGE gels, suggesting covalent protein modifications. Mass spectrometry experiments confirmed that these compounds are nonspecifically alkylating STATs, as well as an unrelated protein, NUDT5. These experiments have highlighted the benefits of PTSA to investigate interactions with STAT proteins and have helped reveal the novel reactivity of Cpd188. The described PTSA represents a promising chemical biology tool that could be applied to an array of other protein targets.
Unusual presentation of aggressive high‐grade B‐cell lymphoma of colonic origin: A case report and review of the literature
Authors: Kosto Amit , Gozlan‐Talmor Aya , Levy Etai , Nalbandyan Karen , Fuchs Lior , Galante Ori 
Published: June 2022

AbstractColonic lymphoma is a rare disease. The presented case is unique, being manifested with abrupt onset, including circulatory shock and lactic acidosis as the initial presentation.
Cephem‐Pyrazinoic Acid Conjugates: Circumventing Resistance in Mycobacterium tuberculosis
Authors: Cole Malcolm S. , Howe Michael D. , Buonomo Joseph A. , Sharma Sachin , Lamont Elise A. , Brody Scott I. , Mishra Neeraj K. , Minato Yusuke , Thiede Joshua M. , Baughn Anthony D. , Aldrich Courtney C. 
Published: June 2022

AbstractTuberculosis (TB) is a leading source of infectious disease mortality globally. Antibiotic‐resistant strains comprise an estimated 10 % of new TB cases and present an urgent need for novel therapeutics. β‐lactam antibiotics have traditionally been ineffective against M. tuberculosis (Mtb), the causative agent of TB, due to the organism's inherent expression of β‐lactamases that destroy the electrophilic β‐lactam warhead. We have developed novel β‐lactam conjugates, which exploit this inherent β‐lactamase activity to achieve selective release of pyrazinoic acid (POA), the active form of a first‐line TB drug. These conjugates are selectively active against M. tuberculosis and related mycobacteria, and activity is retained or even potentiated in multiple resistant strains and models. Preliminary mechanistic investigations suggest that both the POA “warhead” as well as the β‐lactam “promoiety” contribute to the observed activity, demonstrating a codrug strategy with important implications for future TB therapy.
Late‐Stage Functionalisation of Peptides on the Solid Phase by an Iodination‐Substitution Approach
Authors: Werner Marius , Pampel Julius , Pham Truc Lam , Thomas Franziska 
Published: June 2022

AbstractThe functionalisation of peptides at a late synthesis stage holds great potential, for example, for the synthesis of peptide pharmaceuticals, fluorescent biosensors or peptidomimetics. Here we describe an on‐resin iodination‐substitution reaction sequence on homoserine that is also suitable for peptide modification in a combinatorial format. The reaction sequence is accessible to a wide range of sulfur nucleophiles with various functional groups including boronic acids, hydroxy groups or aromatic amines. In this way, methionine‐like thioethers or thioesters and thiosulfonates are accessible. Next to sulfur nucleophiles, selenols, pyridines and carboxylic acids were successfully used as nucleophiles, whereas phenols did not react. The late‐stage iodination‐substitution approach is not only applicable to short peptides but also to the more complex 34‐amino‐acid WW domains. We applied this strategy to introduce 7‐mercapto‐4‐methylcoumarin into a switchable ZnIIresponsive WW domain to design an iFRET‐based ZnIIsensor.
Development of a workflow process mapping protocol to inform the implementation of regional patient navigation programs in breast oncology
Authors: Casanova Nicole L. , LeClair Amy M. , Xiao Victoria , Mullikin Katelyn R. , Lemon Stephenie C. , Freund Karen M. , Haas Jennifer S. , Freedman Rachel A. , Battaglia Tracy A. ,   the Translating Research Into Practice (TRIP) Consortium
Published: June 2022

BACKGROUNDImplementing city‐wide patient navigation processes that support patients across the continuum of cancer care is impeded by a lack of standardized tools to integrate workflows and reduce gaps in care. The authors present an actionable workflow process mapping protocol for navigation process planning and improvement based on methods developed for the Translating Research Into Practice study.METHODSKey stakeholders at each study site were identified through existing community partnerships, and data on each site's navigation processes were collected using mixed methods through a series of team meetings. The authors used Health Quality Ontario's Quality Improvement Guide, service design principles, and key stakeholder input to map the collected data onto a template structured according to the case‐management model.RESULTSData collection and process mapping exercises resulted in a 10‐step protocol that includes: 1) workflow mapping procedures to guide data collection on the series of activities performed by health care personnel that comprise a patient's navigation experience, 2) a site survey to assess program characteristics, 3) a semistructured interview guide to assess care coordination workflows, 4) a site‐level swim lane workflow process mapping template, and 5) a regional high‐level process mapping template to aggregate data from multiple site‐level process maps.CONCLUSIONSThis iterative, participatory approach to data collection and process mapping can be used by improvement teams to streamline care coordination, ultimately improving the design and delivery of an evidence‐based navigation model that spans multiple treatment modalities and multiple health systems in a metropolitan area. This protocol is presented as an actionable toolkit so the work may be replicated to support other quality‐improvement initiatives and efforts to design truly patient‐centered breast cancer treatment experiences.LAY SUMMARY

Evidence‐based patient navigation in breast cancer care requires the integration of services through each phase of cancer treatment.
The Translating Research Into Practice study aims to implement patient navigation for patients with breast cancer who are at risk for delays and are seeking care across 6 health systems in Boston, Massachusetts.
The authors designed a 10‐step protocol outlining procedures and tools that support a systematic assessment for health systems that want to implement breast cancer patient navigation services for patients who are at risk for treatment delays.
What makes for successful patient navigation implementation in cancer prevention and screening programs using an evaluation and sustainability framework
Authors: Dwyer Andrea J. , Staples Elsa S. , Harty Nicole M. , LeGrice Kirsten E. , Pray Shannon L. H. , Risendal Betsy C. 
Published: June 2022

BackgroundIntegrating patient navigation into cancer prevention and early detection efforts has been effective in increasing cancer screening rates for the medically underserved. Few publications have focused on how cancer screening interventions in the primary care setting using patient navigation as a primary strategy are implemented, adapted, and sustained. In 2006, the University of Colorado Cancer Center established a statewide colorectal cancer screening program to reduce disparities and improve colorectal cancer (CRC) screening for the medically underserved in partnership with the safety net system. The program, known as the Colorado Cancer Screening Program (CCSP), has provided navigation into preventive screening for more than 35,000 patients.MethodsTo evaluate sustainability and implementation, the Practical, Robust Implementation and Sustainability Model was used to examine the implementation and impact. To complement this model, the CCSP adapted the Program Sustainability Framework and Clinical Sustainability Framework and Assessment Tools to measure the capacity of participating clinics to sustain the program beyond grant funding.ResultsThe domains most frequently targeted for improvement in the sustainability plans were Workflow Integration (8 clinics), Communication, Planning, and Implementation (8 clinics), and Funding Stability (5 clinics). Activities in the implementation plans included revising workflows for efficiencies, incorporating quality improvement strategies, and building a business case for patient navigation.ConclusionsFindings from this sustainability planning process provide insight about program outcomes, satisfaction, and illustrate that although funding remains one of the key sustainability questions, many clinic systems have prioritized other specific domains to continue sustainability beyond grant funding.
Pediatric oncologists' perspectives on the use of complementary medicine in pediatric cancer patients in Switzerland: A national survey‐based cross‐sectional study
Authors: Pirson Léopold , Lüer Sonja C. , Diezi Manuel , Kroiss Sabine , Brazzola Pierluigi , Schilling Freimut H. , von der Weid Nicolas , Scheinemann Katrin , Greiner Jeanette , Zuzak Tycho Jan , von Bueren André O. 
Published: June 2022

AbstractBackgroundThere is a widespread use of complementary therapies among pediatric cancer patients. Previous studies provided evidence that communication between pediatric oncologists (POs) and patients/families about the use of these therapies is often incomplete. Furthermore, nationwide studies on this topic are rare.AimsWe assessed POs' perspectives on the use of complementary medicine (CM) in Switzerland, on the basis of an edited survey previously used in a nationwide study.Methods and ResultsA link to an online survey was sent by e‐mail to each of the fifty‐two eligible pediatric oncologists in all nine Swiss Pediatric Oncology Group (SPOG) centers. Eligible respondents were board‐certified (Switzerland or abroad) POs currently working at a SPOG center. The survey was available for a total period of 2 months.We received 29 filled questionnaires (overall response rate: 56%). Most POs (59%) indicated that they ask more than 50% of their patients about CM use. Frequent reasons for not asking about the use of CM were i) forgetting to ask (55%), ii) lack of knowledge on the subject (31%), and iii) lack of time (24%). More than every second PO (55%) reported having a lack of knowledge on the subject. A majority of POs (66% to 76%) indicated interest in learning more about specific CM topics (cannabinoids, hypnosis and relaxation, music therapy, herbal medicine, acupuncture, meditation, and yoga). More information and specific training opportunities on the use of CM was deemed important by 76% to 97% of POs.ConclusionPOs working in Switzerland identify complementary therapies as an important subject. Swiss POs are willing to acquire more knowledge on CM. More training seems to be necessary in order to increase awareness about the topic, to enhance communication about complementary therapies and thus to improve patient care.
Corporate social responsibility (environment, product, diversity, employee, and community) and the hotel employees' job performance: Exploring the role of the employment types
Authors: Yu Jongsik , Lho Linda Heejung , Han Heesup 
Published: June 2022

AbstractThis study attempted to verify the influence of hotel corporations' social responsibility activities on perceived job performance. To achieve the purpose of this study, five new sub‐factors (environment, product, diversity, employee, and community) were suggested for the social responsibility activities of hotel corporations. A quantitative analysis was performed to obtain research objectives using SPSS 22 and AMOS 22. Our results evidenced that corporations' social responsibility activities enhanced perceived job performances of the hotel employees. Additionally, employment type of the employees in the relationship between CSR and the job performance showed significant moderating influence. This study provides a very meaningful and important insight that the social responsibility activities of hotel corporations have a significant influence on the improved performance of the hotel employees.
Implementing microwell slides for detection and isolation of single circulating tumor cells from complex cell suspensions
Authors: Yang Liwen , Rivandi Mahdi , Franken André , Hieltjes Maarten , van der Zaag Pieter Jan , Nelep Constantin , Eberhardt Jens , Peter Stefan , Niederacher Dieter , Fehm Tanja , Neubauer Hans 
Published: June 2022

AbstractCell loss during detection and isolation of circulating tumor cells (CTCs) is a challenge especially when label‐free pre‐enrichment technologies are used without the aid of magnetic particles. Although microfluidic systems can remove the majority of “contaminating” white blood cells (WBCs), their remaining numbers are still impeding single CTC isolation, thus making additional separation steps needed. This study aimed to develop a workflow from blood‐to‐single CTC for complex cell suspensions by testing two microwell formats. In the first step, different cell lines were used to compare the performances of Sievewell™ 370 K (TOK, Japan) and CellCelector™ Nanowell U25 (ALS Automated Lab Solutions, Germany) slides for cell labelling and single‐cell micromanipulation. Confounding levels of auto‐fluorescence inherent to different plastic materials used to cast the microwells, staining recovery rates, and cell isolation rates were determined. In the second step, three different blood preservation tubes were tested for RNA analysis. Lastly, the established workflow was applied to isolate CTCs from peripheral blood samples obtained from metastasized breast cancer (mBC) patients for single‐cell DNA and RNA analysis. The detection of CTCs in Sievewell slides profit from better signal‐to‐noise ratios in the fluorescence channels mainly used for CTC detection. In addition, due to its design, Sievewell supports direct in situ CTC labelling, which minimizes cell loss and leads to single‐cell recovery rates after staining of approx. 94%. Detection of PIK3CA mutations in single CTCs verified the applicability of the workflow for the analysis of genomic DNA of CTCs. Furthermore, combined with blood preservation up to 48 h at room temperature in LBguard tubes, panel RT‐PCR transcript analysis was successful for single cell line cells and CTCs, respectively. The combined use of Sievewell microwell slides and CellCelector™ automated micromanipulation system improves single CTC detection, labelling and isolation from complex cell suspensions. This approach is especially valuable when samples of high cellular content are processed.
Organocatalytic One‐Pot Synthesis of Pseudo‐Terpenoids
Authors: Peraka Swamy , Gorachand Badaraita , Hussain Akram , Sravanthi Revoju , Ramachary Dhevalapally B. 
Published: June 2022

AbstractStructurally complex cedrane scaffolds were synthesized in very good yields with high chemo‐ and diastereoselectivities in a sequential one‐pot manner. A combination of intermolecular olefination, intramolecular Michael and Michael reactions or intermolecular olefination, intramolecular Michael and aldol reactions were used as the key steps from the readily available hydroxy‐p‐quinone butanals and phosphoranes with catalytic amounts of quinine at ambient temperatures for a few hours. This is a unique one‐pot combination of coupling and annulation routes for the green synthesis of a library of tricyclic pseudo‐terpenoids (cedrane scaffolds) with high selectivity and yields. Organocatalytic ring isomerization was highlighted through transforming one ring into another one by retro‐cyclization. We have discussed thoroughly mechanistic aspects of these tandem coupling/annulation and ring isomerization reactions based on control experiments and X‐ray crystal structure analysis.
HS‐UNET‐ID: An approach for human skin classification integrating between UNET and improved dense convolutional network
Authors: Nguyen Hai Thanh , Luong Huong Hoang , Phan Phat Tan , Nguyen Hung Huy Duc , Ly Duong , Phan Duc Minh , Do Tin Trung 
Published: June 2022

AbstractThe Sun's ultraviolet radiation, toxic chemicals from industry, and other factors can cause problems to human skin. These factors have boosted the risk of humans contracting skin cancer. In identifying and detecting signals of skin cancer diseases, recent advancements in deep learning techniques significantly improve the accuracy of diagnosis. This work delves into processing techniques for human skin images with MorphologyEx (blackhat), thresholding, semantic segmentation with UNET, and several famous convolutional neural network architectures to improve skin classification performance. If skin cancer can be stopped in the first stage, the survival rate of the patient could be more than 95%, while it drops to just 5% if the detection is carried lately in Stage IV4. Due to its seriousness, the healthcare and research community has received considerable attention, and its most important goal is to be able to diagnose it at an early stage. However, the challenge due to the similarity in melanocytic and non‐melanoma skin lesions is inevitable. We achieved skin classification performances of 0.8560, 0.9672, 0.8063, and 0.8560 measured by overall accuracy, area under curve, Matthews correlation coefficient, and F1‐score, respectively. The proposed approach has been deployed into applications on Mobile and Web platforms.
Hsa_circ_0008726 regulates the proliferation, migration, and invasion of trophoblast cells in preeclampsia through modulating the miR‐1290‐LHX6 signaling pathway
Authors: Zhang Yongyan , Fang Shuai , Wang Jiayi , Chen Siqian , Xuan Rongrong 
Published: June 2022

AbstractBackgroundPreeclampsia (PE) is a serious complication of pregnancy, with a global incidence of about 2%–8%. It is one of the important causes of morbidity and mortality among the pregnant women and perinatal infants. Circular RNA (circRNA) has been confirmed to play an important regulatory role in PE. This study aimed to evaluate the role of hsa_circ_0008726 in the occurrence and development of PE.MethodsThe expression of hsa_circ_0008726 in PE placental tissue and blood was detected by qRT‐PCR. CCK‐8, wound closure, and Transwell assay were used to measure cell proliferation, migration, and invasion. Bioinformatics prediction, Western blotting, and dual‐luciferase reporter gene detection were used to explore the mechanism of hsa_circ_0008726 in HTR8/SVneo cells.ResultsThe expression level of circ_0008726 in the placental tissue and blood samples of PE patients was significantly higher than that of normal controls. The overexpression of circ_0008726 can significantly inhibit the proliferation, migration, and invasion ability of HTR‐8/SVneo cells. While the silence of circ_0008726 showed an opposite effect. Furthermore, hsa_circ_0008726 can modulate the expression of LHX6 by adsorbing miR‐1290.ConclusionHsa_circ_000872 can regulate LHX6 by adsorbing miR‐1290 to inhibit PE progression, thus establishing hsa_circ_000872 as a potential target for predicting and treating PE.
Community participation in a low‐income neighborhood: The salience of sociodemographic characteristics, perceptions and experience of violence, and neighborhood attachment
Authors: Swart Lu‐Anne , Ismail Ghouwa , Seedat Mohamed 
Published: June 2022

AbstractWhile community participation may serve as a vehicle to improve health and socioeconomic outcomes for residents in poor communities, little research exists on the individual factors that influence community participation, particularly in contexts where violence is exceptionally high. This study examined the relationship between sociodemographic characteristics, perceptions, and experiences of violence, neighborhood attachment, and community participation in a low‐income, under‐resourced neighborhood in Johannesburg South Africa. The study used baseline data from 300 randomly selected households and multiple linear regression to assess the relationship between individual factors and community participation. The results suggest a high level of community participation, with religious organizational membership the most common. Being female, employed, and fear of violent crime were positively associated with community participation. Future research should include mixed methods and transdisciplinary research approaches for building our understandings of social justice and transformation‐oriented community participation.
Unusually swift response of relapsed Burkitt leukemia to R‐DHAP
Authors: Harrer Dennis Christoph , Denk Alexander , Keil Felix , Menhart Karin , Mayer Stephanie , Wolff Daniel , Edinger Matthias , Herr Wolfgang , Grube Matthias 
Published: June 2022

AbstractBurkitt leukemia (BL) represents a highly aggressive lymphoma characterized by proliferation rates of around 100%, and a frequent spread into the central nervous system. If standard frontline chemotherapy fails, the prognosis is usually dismal, and reports on successful effective salvage therapy strategies for patients with relapsed/refractory BL are scant. Here, we report on a 40‐year‐old female patient who suffered an early relapse of BL three months after the completion of frontline chemoimmunotherapy. Strikingly, after only one cycle of R‐DHAP chemotherapy, the patient showed CR of BL enabling swift transition to a consolidating allogeneic stem cell transplantation. A 40‐year‐old previously healthy woman presented to the hospital with fatigue and incessant epistaxis, and a diagnosis of BL was made upon histological examination of a bone marrow biopsy. Treatment was initiated according to the GMALL 2002 B‐NHL/ALL protocol, which could induce complete molecular remission. Nevertheless, three months after chemotherapy, the patient exhibited BL relapse in the bone marrow, and on Fluorodeoxyglucose (FDG)–PET‐imaging. The relapse therapy was started with R‐DHAP, and after only one cycle, the patient once again entered complete remission (CR) paving the way for allogeneic stem cell transplantation. Unfortunately, the patient again relapsed five months after transplantation prompting salvage therapy with R‐DHAC and the execution of the second stem cell transplantation. However, one month after the second transplantation the patient presented with chemorefractory meningeosis leukemia resulting in the initiation of palliative care treatment. In summary, we report on rapid CR of relapsed BL after a single cycle of rituximab‐DHAP. Given a paucity of clinical trials on the treatment of patients with r/r BL, we intend to highlight the potential efficacy of rituximab‐DHAP as salvage therapy in those patients.