bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diversity and distribution of lacustrine diatoms along the Trans‐Mexican Volcanic Belt
Authors: Diana Avendaño , Margarita Caballero , Gabriela Vázquez 
Published: December 2022

Abstract

The Trans‐Mexican Volcanic Belt is recognised as a highly biodiverse zone and we present a study of diatom diversity and species distributions in its lakes. We aimed to identify diatom taxa with the highest regional occupancy, explore diversity at a regional level, identify the main variables associated with species distributions, and present weighted average optima for variables that could provide a valuable reference for future palaeoenvironmental research.

We used the relative abundances of diatoms in surficial lake sediments to estimate different diversity metrics and alpha, beta and gamma diversities. Groups of lakes with similar diatom assemblages were identified by means of cluster analysis whereas canonical correspondence analysis (CCA) was used to evaluate the association between diatom composition and climatic, morphometric, hydrochemical and trophic status related variables. Weighted average optima were estimated for salinity and temperature.

From a total of 184 diatom taxa, three were endemic to one or two lakes and eight had the largest regional occupancy, two of which were associated with high salinity environments (Stephanocyclus meneghinianus, Nitzschia frustulum) and the rest with freshwater conditions (Aulacoseira ambigua, A. granulata, A. granulata var. angustissima, Discostella stelligera, Fragilaria crotonensis, Achnanthidium minutissimum). Surprisingly, endemic taxa as well as those with high regional occupancy were mostly planktic or epipelic motile forms, with high dispersal potential. Our results showed 13 clusters of lakes with similar diatom assemblages, in close agreement with beta diversity which predicted the presence of 12 communities. There was high species turnover (βSIM = 0.97) and CCA showed not only that the main environmental variable defining these groups was salinity, but also that temperature, precipitation, depth, and soluble reactive phosphorus were meaningful variables. There was no evidence of an altitude–diversity pattern.

This study emphasises the strong influence that climatic variables have on species distribution when relatively broad geographical areas, with large climatic gradients are analysed.
Clinicopathological and molecular features of indolent natural killer‐cell lymphoproliferative disorder of the gastrointestinal tract
Authors: Yi Hongmei , Li Anqi , Ouyang Binshen , Da Qian , Dong Lei , Liu Yingting , Xu Haimin , Zhang Xiaoyun , Zhang Wei , Jin Xiaofen , Gu Yijin , Wang Yan , Liu Zebing , Wang Chaofu 
Published: December 2022

AimsIndolent natural killer (NK) cell lymphoproliferative disorder of the gastrointestinal (GI) tract (iNKLPD) is a rare, recently recognised neoplasm. Most of the reported tumours are confined to the GI tract, while a small subset of the tumours harbour JAK3 mutations. We collected four cases of iNKLPD with the goal of adding additional information to the current knowledge of this disease regarding the clinicopathological, immunohistochemical and molecular features.Methods and resultsSimilar features including medium‐ to large‐sized lymphoid cells with variable amounts of pale or slightly eosinophilic cytoplasm, and no evidence of EBER, TCR rearrangement were found in four cases. JAK3 K563_C565del mutation was found in one of three cases that were subjected to targeted next‐generation sequencing. Unique findings of our study include one iNKLPD encountered for the first time in nasopharynx, where lesions could be inadvertently diagnosed as extranodal NK/T cell lymphoma, and one iNKLPD located in the gallbladder extended deeply into muscular and adventitial layers. Exceptional CD8‐positive expression was observed in one iNKLPD. In addition, positive staining of phospho‐STAT5, phospho‐STAT3 and phospho‐p38 were found in our cases. None of the four patients received therapy for lymphoma, but all had a benign clinical outcome during a follow‐up time of 20–99 months.ConclusionsWe present four iNKLPDs with clinical, immunohistochemical and molecular features similar to the reported cases, as well as some unusual characters, which expand our knowledge on this disease, and further support the neoplastic nature of iNKLPDs.
The activated partial thromboplastin time‐clot waveform analysis in hemophilia: Does it help in differentiation?
Authors: Mannan Khalid Abdul , Kumar Narender , Ahluwalia Jasmina , Hans Chander , Kler Anita , Prakash Gaurav , Jain Richa 
Published: December 2022

AbstractIntroductionThe clot waveform analysis (CWA) provide valuable information beyond clotting time. The present study was planned to assess whether the activated partial thromboplastin time (aPTT)‐CWA can differentiate between hemophilia A (HA), hemophilia B (HB), or hemophilia A with inhibitors (HAWI).MethodsThe aPTT‐CWA was generated by an optical detection system (ACL‐TOP™ 500 coagulation analyzer) and the other tests were performed as per instructions from the manufacturer in the kit.ResultsA total of 75 samples (47‐HA, 16‐HAWI, and 12‐HB) with prolonged aPTT were recruited. On analyzing the quantitative aPTT‐CWA data of HA (non‐inhibitors) and HB samples, the width of acceleration 1 [+] peak was the differentiating finding. Among the significant parameters, the second derivative [+] peak was lower in both mild and moderate HA, equating to HB. The time for the height of 1/2 fibrin formation and width of velocity was significantly higher in mild, moderate and severe HA. The study did not show any significant differentiating finding while comparing HAWI and hemophilia A non‐inhibitors (HANI). In the subgroups of HAWI and HANI with aPTT <70 s and 70–100 s, the second derivative [+] peak (2A) was higher and the time for the height of 1/2 fibrin formation (1C) was lesser in HAWI.ConclusionThe aPTT‐CWA parameters may be supportive for the differentiation of hemophilia including its severity and the existence of inhibitors.
Knowledge of intensive care unit nurses about medical device‐related pressure injury and analysis of influencing factors
Authors: Fu Fang , Zhang Lanlan , Fang Jiexin , Wang Xiaoli , Wang Fangfang 
Published: December 2022

AbstractTo investigate ICU nurses' knowledge level with regard to device‐related pressure injuries in northern, central, and southern China and analyse its influencing factors. A total of 261 ICU nurses participated in this cross‐sectional survey A convenience sampling method was used to select ICU nurses as respondents from one hospital in each of the six cities of Taiyuan, Wuhan, Xianning, Guangzhou, Foshan, and Huizhou. Data were collected using the MDRPI Knowledge Questionnaire. The questionnaire was developed by the investigators based on a summary of evidence of MDRPI, which has been reviewed and validated by experts. The obtained data were analysed using SPSS software. The average rate of the correct response about MDRPI was 60.54% (15.74 ± 2.90). The lowest percentage of correct responses was on the “concept and staging” dimension rated 28% (0.56 ± 0.67). The “skin assessment” dimension rated 39.2% (1.57 ± 0.84). Multiple linear regression analysis showed that the factors influencing the MDRPI knowledge of ICU nurses included hospital grade, the highest educational attainment, whether or not they had wound care certification, when they last attended MDRPI training or lectures, and whether or not they had attended MDRPI training or lectures. The level of knowledge of nurses about MDRPI was insufficient. Training of ICU nurses on MDRPI should be emphasised at the institutional level. MDRPI training contents should be based on clinical evidence and updated timely. There is a need to focus on the training of wound care certification and education.
Microstructure and property evolution of high‐entropy rare‐earth silicate T/EBCs during thermal aging
Authors: Fan Dong , Zhong Xin , Zhang Zhenzhong , Huang Liping , Niu Yaran , Wang Lujie , Li Qilian , Zheng Xuebin 
Published: December 2022

AbstractWith the increasing service temperature of aeroengines, the development of thermal/environmental barrier coatings (T/EBCs) has been considered to be a great challenge, which requires high‐temperature stability and excellent thermal and mechanical properties. In this work, high‐entropy design was adopted to change the crystal structure of rare‐earth monosilicates used for T/EBCs. The microstructure evolution, thermal and mechanical properties of the plasma‐sprayed (Lu0.25Yb0.25Er0.25Y0.25)2SiO5and (Lu0.2Yb0.2Er0.2Ho0.2Y0.2)2SiO5coatings before and after thermal aging at 1350°C for 50 h were investigated. Results showed that the as‐sprayed coatings exhibited dense structure with amorphous and decomposed phases. The change of microstructure like amorphous crystallized and defect healing occurred after thermal aging, which had obvious influence on thermomechanical properties. Compared with single‐component RE2SiO5(RE = Yb, Er, Y) coatings, the thermal‐aged high‐entropy coatings exhibited lower thermal conductivity, similar thermal expansion coefficient, and better toughness. The work will provide a foundation for the design and application of T/EBCs materials.
Enhancement of piezoelectric properties of BF–BT ceramics by using ZrO2 powder bed during the sintering process
Authors: Zhang Yi , Huang Rongxia , Zhang Fan , Liang Zhihao , Xie Tianbao , Lin Hua‐Tay , Dai Yejing 
Published: December 2022

AbstractZrO2 powders of various particle sizes (0.15, 0.7, 500 µm) were used to simulate loose powder bed sintering to prepare BF–BT piezoelectric ceramics. The phase structure, dielectric properties, ferroelectric properties, and piezoelectric properties were compared with the samples sintered by the conventional powder bed method (i.e., powder of the same composition as the sample). Results showed that the use of loose ZrO2 powder bed could improve the heat conduction rate and the sintering quality of bulk BF–BT piezoelectric ceramics. The XPS results showed that the samples sintered with 500 µm ZrO2 powder beds had the lowest concentration of Fe2+, exhibited the largest piezoelectric coefficients (d33 = 201 pC/N). In contrast, the sample sintered with a conventional powder bed under the same sintering conditions had a piezoelectric coefficient d33 of 156 pC/N.
Structure and properties of cerium phosphate and silicophosphate glasses
Authors: Xia Yang , Calahoo Courtney , Rodrigues Bruno Poletto , Griebenow Kristin , Graewe Lennart , Wondraczek Lothar 
Published: December 2022

AbstractWe report on the fabrication, properties, and structure of cerium pyrophosphate glasses and partially substituted cerium silicophosphates. In those glasses, cerium occurs predominantly as Ce(III). A combination of dynamic nuclear magnetic resonance and electrical impedance spectroscopy is used to overcome the problem of assessing cerium speciation. While optical spectroscopy is unable to quantify the ratio of Ce(III)/Ce(IV) due to spectral overlap, proxy observations of the effect of silica‐for‐cerium substitution on optical extinction and the shape and width of the UV band gap corroborate vibrational spectroscopic data of the structural roles of cerium and silica. While silica bonding to phosphate units appears to stabilize Ce(IV), it also impedes the polaron transport, leading to higher polaron activation energy and lower electronic conductivity. On the other hand, Ce(III) is stabilized by coordinating to P = O.
Metabolomic and transcriptomic studies of improvements in myocardial infarction due to Pycr1 deletion
Authors: Xue Zhimin , Pan Yiwen , Kong Xugang , Zhang Jiefang , Wu Danyu , Zhou Binquan 
Published: December 2022

AbstractMyocardial infarction (MI) remains a major challenge to cardiovascular health worldwide, with poor healing leaving a direct impact on patients' quality of life and survival. Metabolic abnormalities after MI are receiving increasing attention. Our previous studies showed that enhancing proline catabolism ameliorates hypoxic damage to myocardial cells; therefore, we sought to determine whether reducing the synthesis of endogenous proline also affects MI. We analysed GEO datasets associated with MI and western blot of mouse heart tissue in an MI model to demonstrate pyrroline‐5‐carboxylate reductase 1 (Pycr1) expression level after MI. We constructed Pycr1 KO mice by CRISPR/Cas9 technology to explore the effect of Pycr1 gene KO after MI using transcriptomic and metabolomic techniques. In this study, we found reduced mRNA and protein expression levels of Pycr1 in the hearts of mice after MI. We observed that Pycr1 gene KO has a protective effect against MI, reducing the area of MI and improving heart function. Using transcriptomics approaches, we found 215 upregulated genes and 247 downregulated genes after KO of the Pycr1 gene, indicating that unsaturated fatty acid metabolism was affected at the transcriptional level. Metabolomics results revealed elevated content for 141 metabolites and decreased content for 90 metabolites, among which the levels of fatty acids, glycerol phospholipids, bile acids, and other metabolites increased significantly. The changes in these metabolites may be related to the protective effect of Pycr1 KO on the heart after MI. Pycr1 gene KO has a protective effect against MI and our research will lay a solid foundation for the development of future Pycr1‐related drug targets.
Association of cardiovascular health metrics with annual incidence of prediabetes or diabetes: Analysis of a nationwide real‐world database
Authors: Okada Akira , Kaneko Hidehiro , Matsuoka Satoshi , Itoh Hidetaka , Suzuki Yuta , Fujiu Katsuhito , Michihata Nobuaki , Jo Taisuke , Takeda Norifumi , Morita Hiroyuki , Yamaguchi Satoko , Node Koichi , Yamauchi Toshimasa , Yasunaga Hideo , Komuro Issei 
Published: December 2022

ABSTRACTAims/IntroductionLittle is known about the relationship between cardiovascular health (CVH) metrics and the risk of developing prediabetes or diabetes. We examined the association of CVH metrics with the annual risk of developing prediabetes or diabetes.Materials and MethodsWe carried out this study including 403,857 participants aged 18–71 years with available data on fasting plasma glucose (FPG) data for five consecutive years and with normal FPG (<100 mg/dL) at the initial health checkup. We identified the following ideal CVH metrics: non‐smoking, body mass index of <25 kg/m2, maintaining physical activity, taking breakfast, untreated blood pressure of <120/80 mmHg and untreated total cholesterol of <200 mg/dL. We defined the primary end‐point as prediabetes (FPG 100–125 mg/dL) or diabetes (FPG ≥126 mg/dL or use of antihyperglycemic medications). We examined the relationship of CVH metrics with the annual incidence of prediabetes or diabetes. Additionally, we examined the association of 1‐year changes in CVH metrics with the risk for prediabetes or diabetes.ResultsThe median age was 44 years, and 65.6% were men. An increasing number of non‐ideal CVH metrics was associated with an elevated risk of prediabetes or diabetes. A non‐ideal body mass index was most strongly associated with the risk of prediabetes or diabetes. The risk of developing prediabetes or diabetes rose as the number of non‐ideal CVH metrics increased over 1 year.ConclusionsCVH metrics could stratify the risk of the annual development of prediabetes or diabetes. The risk of developing prediabetes or diabetes might be reduced by improving CVH metrics.
Can markers of disease severity improve the predictive power of claims‐based multimorbidity indices?
Authors: Rizzo Anael , Jing Bocheng , Boscardin W. John , Shah Sachin J. , Steinman Michael A. 
Published: December 2022

AbstractBackgroundClaims‐based measures of multimorbidity, which evaluate the presence of a defined list of diseases, are limited in their ability to predict future outcomes. We evaluated whether claims‐based markers of disease severity could improve assessments of multimorbid burden.MethodsWe developed 7 dichotomous markers of disease severity which could be applied to a range of diseases using claims data. These markers were based on the number of disease‐associated outpatient visits, emergency department visits, and hospitalizations made by an individual over a defined interval; whether an individual with a given disease had outpatient visits to a specialist who typically treats that disease; and ICD‐9 codes which connote more versus less advanced or symptomatic manifestations of a disease. Using Medicare claims linked with Health and Retirement Study data, we tested whether including these markers improved ability to predict ADL decline, IADL decline, hospitalization, and death compared to equivalent models which only included the presence or absence of diseases.ResultsOf 5012 subjects, median age was 76 years and 58% were female. For a majority of diseases tested individually, adding each of the 7 severity markers yielded minimal increase in c‐statistic (≤0.002) for outcomes of ADL decline and mortality compared to models considering only the presence versus absence of disease. Gains in predictive power were more substantial for a small number of individual diseases. Inclusion of the most promising marker in multi‐disease multimorbidity indices yielded minimal gains in c‐statistics (<0.001–0.007) for predicting ADL decline, IADL decline, hospitalization, and death compared to indices without these markers.ConclusionsClaims‐based markers of disease severity did not contribute meaningfully to the ability of multimorbidity indices to predict ADL decline, mortality, and other important outcomes.
Details matter in quality science. Adopting a checklist for magnetic resonance spectroscopy can help you get them right
Authors: Rae Caroline D. , Williams Stephen R. 
Published: December 2022

AbstractThe ISMRM study group on magnetic resonance spectroscopy has produced recommendations for reporting methods. The Journal of Neurochemistry has decided to encourage the use of the checklist for these standards by authors and reviewers in order to improve reproducibility and reliability of the science, make it easier for reviewers and to help educate the scientific community. Here, we explain why getting the details right is important.image
A cautionary tale: Alien prolactins may induce lesser, no, or opposite effects to homologous hormone!
Authors: Barbee Sadie , Radecki Kelly C. , Lorenson Mary Y. , Walker Ameae M. 
Published: December 2022

AbstractCost and availability have often dictated the use of heterologous/alien prolactins in experiments, particularly in vivo. The assumption has been that what is initiated in the target cell is representative of the homologous hormone since many heterologous mammalian prolactins bind to and activate rodent receptors. Here, we examined gene expression in mouse liver in response to a 7‐day treatment with recombinant mouse prolactin (mRecPRL), recombinant ovine prolactin (oRecPRL) and pituitary extract ovine prolactin (oPitPRL). Having established mouse ribosomal protein S9 as the most stable reference gene in the liver in the absence and presence of prolactin treatment, we examined expression of the two most highly expressed prolactin receptors (PRLRs) and three members of the Cyp3a group of cytochrome P450 isoenzymes by qRTPCR. For short form (SF) 3 PRLR, mRecPRL doubled expression while for oRecPRL and oPitPRL expression was only 1.3‐fold control. For the long form (LF) PRLR, changes were similar to those seen for SF 3 PRLR, such that the SF3:LF PRLR ratio remained the same. Expression of the Cyp3as was also dependent on the prolactin origin and, although mRecPRL always stimulated, the other PRLs caused varying results. Compared to control, Cyp3a16 was stimulated 12‐fold by mRecPRL, 3‐fold by oRecPRL, and 6‐fold by oPitPRL. For Cyp3a41, mRecPRL was 3.7‐fold control, oRecPRL was without effect, and oPitPRL was 2‐fold control. Importantly, for Cyp3a44, mRecPRL stimulated 2‐fold, whereas both oRecPRL and oPitPRL had an opposite, inhibitory effect, with expression at 0.5‐fold control. We conclude that homologous hormone had the largest stimulatory effect on expression of all measured genes and that by contrast heterologous hormone showed reduced activity, no activity, or opposite activity, depending on the gene being analyzed. Thus, experimentation using alien heterologous PRL may lead to inaccurate conclusions.
The effect of macro‐control and organizational support perception on nurses and physicians intention to quit during the COVID‐19 pandemic
Authors: Çetin Aslan Emine , Türkmen İbrahim , Top Mehmet 
Published: December 2022

AbstractAimThe aim of this study is to determine the effect of organizational support and COVID‐19 macro‐control perception levels on intention to quit in physicians and nurses.BackgroundDuring the pandemic process, healthcare workers faced great health risks and workloads. Many health workers are considering quitting their job.MethodA cross‐sectional and explanatory research. The sample of the study consisted of 300 nurses and 73 physicians. To collect data, COVID‐19 perception of macro‐control, perceived organizational support, and intention to quit scales were used. Structural equation modeling was used.ResultsCOVID‐19 macro‐control perception and perceived organizational support have a negative effect on the intention to quit. The perceived organizational support has a partial mediating role, on the COVID‐19 macro‐control perception and the intention to quit.ConclusionThis study demonstrated that the macro measures taken against the pandemic and the organizational support received by the nurses and physicians, who are the main actors in the fight against the epidemic, negatively affected their intention to leave.Implications for nursing practice and clinical relevanceTo reduce the intention to quit health workers during the COVID‐19 pandemic, scientific‐based macro‐control measures should be taken, and organizational support should be provided.