bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction and Motif Analysis of 2’-O-methylation Using a Hybrid Deep
Learning Model from RNA Primary Sequence and Nanopore Signals
Authors: Pan Shiyang , Zhang Yuxin , Wei Zhen , Meng Jia , Huang Daiyun 
Published: August 2022

Background:
2’-O-Methylation (2’-O-Me) is a post-transcriptional RNA modification that
occurs in the ribose sugar moiety of all four nucleotides and is abundant in both coding and non-coding
RNAs. Accurate prediction of each subtype of 2’-O-Me (Am, Cm, Gm, Um) helps understand their role
in RNA metabolism and function.

Objective:
This study aims to build models that can predict each subtype of 2’-O-Me from RNA sequence
and nanopore signals and exploit the model interpretability for sequence motif mining.

Methods:
We first propose a novel deep learning model DeepNm to better capture the sequence features
of each subtype with a multi-scale framework. Based on DeepNm, we continue to propose HybridNm,
which combines sequences and nanopore signals through a dual-path framework. The nanopore signalderived
features are first passed through a convolutional layer and then merged with sequence features
extracted from different scales for final classification.

Results:
A 5-fold cross-validation process on Nm-seq data shows that DeepNm outperforms two stateof-
the-art 2’-O-Me predictors. After incorporating nanopore signal-derived features, HybridNm further
achieved significant improvements. Through model interpretation, we identified not only subtypespecific
motifs but also revealed shared motifs between subtypes. In addition, Cm, Gm, and Um shared
motifs with the well-studied m6A RNA methylation, suggesting a potential interplay among different
RNA modifications and the complex nature of epitranscriptome regulation.

Conclusion:
The proposed frameworks can be useful tools to predict 2’-O-Me subtypes accurately and
reveal specific sequence patterns.
A Review of Structural Features, Biological Functions and Biotransformation
Studies in Adipose Tissues and an Assessment of Progress and Implications
Authors: Yang Mao Sheng , Chu Ting 
Published: August 2022

Abstract:
Roles for adipose tissues in energy metabolism, health maintenance and disease onset
have been established. Evidence indicates that white, brown and beige fats are quite different in
terms of their cellular origin and biological characteristics. These differences are significant in targeting
adipocytes to study the pathogenesis and prevention strategies of related diseases. The biotransformations
of white, brown and beige fat cells constitute an intriguing topic worthy of further
study, and the molecular mechanisms underlying the biotransformations of white, brown and beige
fat cells remain to be elucidated. Hence, we herein collected evidence from studies on adipose tissue
or adipocytes, and we extracted the structural features, biologic functions, and biotransformations
of adipose tissue/adipocytes. The present review aimed to summarize the latest research progress
and propose novel research directions with respect to adipose tissue and adipocytes. We posit that
this work will provide new insights and opportunities in the effective treatment strategies for obesity,
diabetes and other lipid-related diseases. It will also contribute to our knowledge of the basic
biologic underpinnings of adipocyte biology.
Exploring Cytokines as Potential Target in Peptic Ulcer Disease: A Systematic
Update
Authors: Doshi Gaurav Mahesh , Ansari Alveera Zubair , Bhatia Nirav Yogesh , Gharat Sankalp Ashok , Godad Angel Pavalu 
Published: August 2022

Abstract:
Peptic ulcer disease (PUD) is a widespread condition that affects millions of people
each year, with an incidence rate of 0.1%-1.5%, and has a significant impact on human health.
A range of stimuli, such as Helicobacter pylori, non-steroidal anti-inflammatory drugs, hyperacidity,
stress, alcohol, smoking, and idiopathic disease states, can produce a sore in the gastrointestinal
mucosal layer. For individuals infected with H. pylori, 2%-3% remain asymptomatic
throughout their life. Although PUD treatments are available, genetic variations occurring in
individuals because of geographical dissimilarity and antibiotic resistance pose limitations. Specifically,
inflammatory cytokine gene polymorphisms have received immense attention in recent
years because they appear to affect the severity and duration of stomach inflammation,
which is induced by H. pylori infection, contributing to the initiation of PUD. In such a context,
in-depth knowledge of interleukins may aid in the discovery of new targets and provide precautionary
approaches for the treatment of PUD. This review aims to give insights into the importance
of several interleukins that cognate with PUD and contribute to ulcer progression or
healing by activating or dampening the host immunity. Furthermore, the available targets with
clinical evidence have been explored in this review.
The COVID 19 Pandemic-the Final Straw for Irish Rheumatology Services?
Authors: Mulkerrin Patrick , Durcan Laura , Chavrimootoo Shawn , Kane David , Killeen Gary 
Published: August 2022

Introduction:
The COVID-19 pandemic has caused disruption to the worldwide provision of acute and chronic care to patients. The effect has been particularly marked in rheumatology in Ireland, where the ability to provide acute and chronic care has been dramatically curtailed due to the combined effects of social distancing, staff redeployment and the repurposing of rheumatology units. Prior to the pandemic, there were significant challenges from an infrastructural and staffing level in Irish rheumatology.

Methods:
Using a questionnaire, the authors evaluated the effect of the first wave of the COVID 19 pandemic on rheumatology services.

Results:
Responses from 87% of Rheumatology specialist services in Ireland indicate that 83% of review appointments were remote, with 87% of new patient assessments in OPD were “in person”. Only 41% of usual outpatient activity could occur within existing infrastructure given guidelines re social distancing which would result a shortfall of 42000 patient appointments. Significant numbers of staff were reassigned from Rheumatology to services elsewhere.

Conclusion:
The COVID-19 pandemic has had a significant negative impact on access to struggling Irish Rheumatology services. Implementation of agreed recommendations for augmenting services must occur urgently.
Problematic Social Media Use and Academic Performance among University Students: An Evaluation from The Middle East
Authors: Alhusban Ahmed , Mismar Thabet , Al Husban Abdalla , Alzoubi Karem H. 
Published: August 2022

Background:
The widespread use of social media applications generated a problematic behavior of excessive and inappropriate use that has been associated with mental health problems. Available data assessed this behavior using different surrogate markers in certain university majors. This study aims to assess the effect of this behavior on academic performance, using a validated tool, across different majors.

Methods:
A cross-sectional study that randomly recruited university students from three Middle Eastern countries. using an online survey. The study included 277 participants with an average age of 21.53±2.1 years. The problematic social media use (PSMU) was evaluated using the Bergen Social Media Addiction Scale (BSMAS) and academic performance was evaluated using the GPA. Data regarding the demographics and the characteristic of social media use were collected.

Results:
PMSU was identified as an independent predictor of academic performance. The low academic performance group was more likely to use social media applications during the night, which negatively affected the ability to wake up the next day and be ready for exams.

Conclusion:
There is a need for a more large-scale systematic evaluation of the extent of PSMU and its effect on academic performance among university students at both the regional and international levels. These analyses will help in building effective interventions to reduce the impact of PSMU on university students.
Online Customer Behavior: An Analysis of the Effects of Cognitive and
Affective Trust
Authors: Foroutan Reza Alizadeh , Sarokolaei Mahmoud Gholami , Rezaeian Javad 
Published: August 2022

Background:
Although many scholars have argued that the key factor for success in the
e-commerce is the deployment of a trusted and reliable process for the buyer, the meaning of customer
trust in e-commerce and the factors affecting it are not still well-known and to be investigated.
Understanding how customers build their trust and developing a trust model is beneficial to increase
the ability to do so.

Objective:
The aim of this study was to investigate whether the factors such as cognitive and affective
trust can affect customer behavior in online shopping or not.

Method:
Questionnaire was used to collect data and to check their normality, and the Kolmogorov–
Smirnov test was applied. Cronbach’s alpha was employed to test the reliability, and the Structural
Equation Model (SEM) was utilized to confirm or reject the hypotheses. Inferential statistical
analysis was performed using SmartPLS software.

Results:
While perceived website quality can affect cognitive trust, variables like security and privacies
policy, and shared value of a website can influence affective trust. In addition, there are variables
like prior-interaction experience, and perceived reputation of an e-tail which can impact on
both cognitive and affective trust. On the other hand, it was shown that both cognitive and affective
trust can influence customer satisfaction; a variable which along with internet usage experience,
and online shopping experience affect loyalty intention.

Conclusion:
The empirical results show that cognitive and affective trust are prominent variables
in online retailing. They mediate between PWQ, SPP, PIE, PETR, and SV on the one hand and CS
on the other hand. In addition, CS influences LI. The same is true for OSE and IE.
Viral Infectivity Inhibition and Viral Biological Elements Destruction using Safe and Low Power Electrons Generated by Life Restoration Device
(LRD): An In Vitro Study
Authors: Madboly Waleed Ezzat , Abu-Dief Ahmed M. 
Published: August 2022

Background:
Recently, different side effects have been observed after using antiviral
drugs before activation of the immune system. Therefore, it is very important to use effective and
non-invasive therapy with fewer side effects for infected virus treatment.

Methods:
In this study, we designated a new device termed a Life Restoration Device (LRD). The
main function of LRD is to generate electric frequencies with lower and safer potential. These frequencies
can effectively destroy the biological elements in the viruses, such as nucleic acid materials
and viral cell membranes, but not the cellular plasma membrane of the infected eukaryotic cells.

Results:
A designated glass tube was prepared for this purpose. The infected cell culture was located
in the cell culture media, and propagated viruses were poured into the glass tube. Additionally,
two nickel-coated copper rods were inserted into both ends of the tube inside the cell culture media.
Afterward, the two nickel-coated copper rods were connected to the LRD. Using LRD, lower potential
electric frequencies were generated and applied for 30 min and 60 min time points. The treatment
of the cell culture containing MERS-CoV and SARS-CoV-2 with LRD for 30 min significantly
reduced the viral infectivity by 83% and 22%, respectively. After 60 min of treatment with LRD,
the infectivity of MERS-CoV and SARS-CoV-2 viruses was reduced by 21% and 1%, respectively.
Furthermore, HIV and HBV-infected blood showed a 95.5% and 100% viral inhibition rate after 2 h
exposure to LRD. Additionally, based on the results of the electron microscopy of treated H5N1 virus
and western blot analysis data of different types of viruses, the nucleic acid components of
the treated viruses were reduced compared to the non-treated viruses. The low-power electric frequencies
produced by LRD can reduce the fluidi ty and osmosis of the viral envelope but not
the plasma membrane of the infected cells.

Conclusion:
Treatment of different types of pathogenic viruses with electric stimulation produced
by LRD is a new alternative to safe therapy but needs further investigations. The results of
this study are important to develop an effective, safe, and alternative viral therapy.
An Updated Review on Traditional and Modern Aspects of Vitex negundo
Authors: Husain Syed Akhtar , Perveen Summaya , Khan Mohammad Aasif , Parveen Rabea , Insaf Areeba , Parveen Bushra , Ahmad Sayeed 
Published: August 2022

Abstract:
Vitex negundo is a traditional herb known for its medicinal properties in Unani, Ayurveda,
Siddha, Chinese, Roman, and other traditional systems of medicine. Traditionally, it is used as
anthelminthic, antitoxin, aphrodisiac, contraceptive, antimalarial, analgesic, antiinflamatory, antiasthmatic,
vermifuge, etc. All parts of V. negundo contain a number of phytoconstituents like alkaloids,
fatty acids, flavonoids, glycosidic irridoids, lignans, phenols, steroids, tannins and di- and sesquiterpenes.
Due to the presence of a variety of secondary metabolites, V. negundo is used in different
types of diseases or disorders by traditional practitioners for the treatment of spermatorrhoea,
stomachache, asthma, cold, diarrhoea, indigestion, gallstone, hernia, eye disorders, rheumatism, irritable
bladder and dysmenorrhea, headache, migraine, kwashiorkor, neck gland sores, tubercular
neck swelling, reddened, arthritis, jaundice, urticaria, eczema and liver disorders. It is most widely
used for curing disorders of the reproductive system like vital power, depression, frail erection
without libido, self-contempt for sexual abuse, stool containing prostatic fluid, and testicle pain.
Facts were gathered from databases such as PubMed, Google Scholar, Springer, Scopus, and ScienceDirect,
as well as literature found in books on medicinal plants. It has recently been reported to
have cytotoxic properties against various types of cancer cells. It works by inducing apoptosis via
the mitochondrial and TRIAL pathways and stopping the cell cycle. This review sheds light upon
the ethnomedicinal uses, phytochemistry, different extraction procedures, and pharmacology of V.
negundo. In conclusion, it was found that this traditional herb had an emerging past, exciting present
and promising future for the treatment of several diseases.
Study on Intelligent Analysis and Processing Technology of Computer Big
Data Based on Clustering Algorithm
Authors: Kumar ER Rakesh , Liu Xiaoming , Rokunojjaman Md , Nazila Ragimova , Vugar Abdullayev 
Published: August 2022

Aim:
Clustering belongs to unsupervised learning, which divides the data objects into the
data set into multiple clusters or classes, so that the objects in the same cluster have high similarity

Background:
The clustering of spatial data objects can be solved by optimization based on the clustering objective function.

Objective:
Study on intelligent analysis and processing technology of computer big data based on
clustering algorithm.

Methods:
First, a new dynamic self-organizing feature mapping model is proposed, and the training
algorithm of the model is given. Then, the spectral clustering technology and related concepts are
introduced. The spectral clustering algorithm is studied and analyzed, and a spectral clustering algorithm that automatically determines the number of clusters is proposed. Furthermore, an algorithm
for constructing a discrete Morse function to find the optimal solution is proposed, proving that the
constructed function is the optimal discrete Morse function. At the same time, two optimization
models based on the discrete Morse theory are constructed. Finally, the optimization model based
on discrete Morse theory is applied to cluster analysis, and a density clustering algorithm based on
the discrete Morse optimization model is proposed.

Results:
This study is focused on designing and implementing a partitional-based clustering algorithm based on big data, that is suitable for clustering huge datasets to meet low computational requirements. The experiments are conducted in terms of time and space complexity and it is observed that the measure of clustering quality and the run time is capable of running in very less time
without negotiating the quality of clustering. The results show that the experiments are carried out
on the artificial data set and the UCI data set.

Conclusion:
The efficiency and superiority of the new model, are verified by comparing it with the
clustering results of the DBSCAN algorithm.
Feigning Capgras Syndrome and Developmental Prosopagnosia:
A Rare Case of Pediatric Factitious Disorder
Authors: Colizzi Marco , Antolini Giulia , Puttini Elena , Bortoletto Riccardo , Begnini Carolina , Zoccante Leonardo 
Published: August 2022

Background:
Factitious Disorder is a condition included among psychiatric disorders,
where symptoms are intentionally produced to seek medical attention. Its epidemiology,
treatment, and outcome are largely unknown.

Objective:
To increase knowledge of the condition, we discuss a pediatric case where the patient
feigned neuropsychiatric symptoms.

Methods:
A 15-year-old female adolescent presented with neuropsychiatric symptoms suggestive
of rare conditions such as Delusional Misidentification Syndrome (i.e., Capgras and
Fregoli syndromes) and Developmental Prosopagnosia as well as epileptic seizures.

Results:
When Delusional Misidentification Syndrome was suspected, she was treated with
Risperidone. Due to suboptimal response, Alprazolam and Sertraline were added and then
Risperidone was cross-titrated to Haloperidol. The condition severity led to her admission to an
inpatient Child and Adolescent Neuropsychiatry Unit. MRI scan and electroencephalographic
recording were normal. The self- and parent-reported psychological assessment indicated several
psychiatric symptoms. Instead, the clinician-administered Minnesota Multiphasic Personality
Inventory-Adolescent and Structured Inventory of Malingered Symptomatology suggested
a fake-bad profile and neuropsychiatric symptom simulation. Along with evidence of incongruent
symptoms between the patient’s interviews, such investigations led to the diagnosis of
Factitious Disorder. Psychopharmacological treatment was discontinued, and psychological
support was offered to the patient and her parents.

Conclusion:
Increasing knowledge about Factitious Disorder is needed.
Probiotic influences on motor skills
Authors: Lalonde Robert , Strazielle Catherine 
Published: August 2022

Abstract:
Probiotics are bacteria whose favorable physiological properties have mostly been demonstrated in regard to anxiety and stress. More recent experiments indicate potential use of single- and multi-strain probiotics on motor-related diseases. Initial studies in patients with Parkinson’s disease and Prader-Willi syndrome are concordant with this hypothesis. In experiments with rodents, probiotics improved motor coordination in normal animals and models of Parkinson’s disease, multiple sclerosis, and spinal cord injury as well as grip strength in hepatic encephalopathy. Further studies should delineate the bacterial profile of each condition and their experimental models.
Mechanism-Based Inhibition of CYP3A Subfamilies by Macrolide
Antibiotics and Piperine
Authors: Niwa Toshiro , Ishii Risa 
Published: August 2022

Objective:
The mechanism-based inhibition of macrolide antibiotics, such as erythromycin and
clarithromycin, and piperine on testosterone 6β-hydroxylation activities by cytochrome P450 (CYP) 3A4,
polymorphically expressed CYP3A5, and fetal CYP3A7 were compared.

Methods:
6β-Hydroxy
testosterone was determined by high-performance liquid chromatography.

Results:
Although
preincubation with erythromycin and clarithromycin decreased CYP3A4-meditaed testosterone 6β-
hydroxylation in a time-dependent manner, and the estimated maximum inactivation rate constant (k inact )
and the inactivation rate constant reaching half of k inact (K i ) for erythromycin were approximately 1/2 and 1/5, respectively, of those for clarithromycin. Obvious preincubation time-dependent inhibition of
erythromycin against CYP3A5 and CYP3A7 was not observed. Piperine exhibited preincubation time-
dependent inhibition, and the calculated K i and k inact values for CYP3A4 were approximately 1/7 and 1/2,
respectively, of those for CYP3A5.

Conclusion:
It is speculated that the preincubation-dependent
inhibition by piperine would be more potent in CYP3A5 non-expressors than CYP3A5-expressors.
Efecto de una intervención educativa virtual para promover el inicio temprano de la lactancia materna en embarazadas de Lima, Perú
Authors: Ortiz Romaní Katherine Jenny , Vásquez Carbajal Angella Maricielo , Escobedo Encarnación Josselyne Rocío , Ortiz Montalvo Yonathan Josué 
Published: August 2022

Objetivo: El presente trabajo de investigación consistió en evaluar el efecto de una intervención educativa virtual para promover el inicio temprano de la lactancia materna (ITLM) en gestantes usuarias del centro de salud Los Libertadores, ubicado en la ciudad de Lima (Perú), en el año 2021. Materiales y métodos: Se realizó un estudio analítico, longitudinal y prospectivo de método intervención educativa virtual a gestantes de un establecimiento de salud de Lima en el período de 26 de enero a 9 de febrero de 2021. La muestra estuvo conformada por 40 gestantes residentes en Lima. Para la recolección de datos se empleó un cuestionario conformado por 12 preguntas, este fue previamente validado por cinco jueces expertos y con buena confiabilidad: el Alfa de Cronbach resultó 0.90. Asimismo, el rotafolio tuvo un Alfa de Cronbach de 0.831. Resultados: Hubo un aumento en los conocimientos tras la intervención educativa (p<0.05). Y el 83.33 % de las gestantes que pertenecieron al grupo experimental realizaron el inicio temprano de lactancia materna después de la intervención (p<0.001). Conclusión: La intervención educativa virtual tuvo un impacto positivo en el aumento de conocimientos de las embarazadas sobre el inicio temprano de la lactancia materna en el grupo experimental, y cuando fueron madres realizaron el ITLM. Por ende, se recomienda que se debe promover esta temática desde la atención prenatal.
Impact of a Novel Diabetes Prevention Intervention for Early Slow Weight Loss Responders Among Adults With Prediabetes: An Adaptive Trial
Authors: Miller Carla K. , Nagaraja Haikady N. , Cheavens Jennifer , Fujita Kentaro , Lazarus Sophie 
Published: August 2022

OBJECTIVE
Some people are slower to respond during lifestyle interventions. An adaptive “rescue” intervention may improve outcomes among slow responders. The impact of a worksite rescue intervention for early slow responders was evaluated.

RESEARCH DESIGN AND METHODS
Employees ≥21 years old with prediabetes were stratified to intervention using a 2.5% weight loss (%WL) threshold at week 5. Outcomes were assessed at baseline and at 4 months using mixed-effect and linear regression models.

RESULTS
Significant improvement occurred in mean %WL, glycemia, total cholesterol, and triglycerides in the standard compared with the adaptive (Group Lifestyle Balance Plus [GLB+]) intervention (all P≤ 0.01). However, GLB+ participants also experienced a significant reduction in %WL and glycemia (all P &lt; 0.01). The %WL at week 5 significantly predicted %WL at 4 months (P &lt; 0.0001). The between-group difference of 4-month %WL was not significant for someone achieving 2.5%WL at week 5.

CONCLUSIONS
Diabetes prevention programs should consider weight loss success following 1 month of treatment and offer a rescue intervention to early slow weight loss responders.