bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic potential of recombinant human basic fibroblast growth factor on postoperative patients with chronic rhinosinusitis with nasal polyps
Authors: Wu Jian , Lai Yinyan , Wang Kanghua , Lai Ming , Jiang Lijie , Xu Zhaofeng , Shi Jianbo , Gao Wenxiang 
Published: July 2022

AbstractObjectivesTo explore the effect of intranasal administration of recombinant human basic fibroblast growth factor (rh‐bFGF) on postoperative chronic rhinosinusitis with nasal polyps (CRSwNP) patients.DesignA prospective, randomised, controlled, single‐blinded trial.Setting and ParticipantsSeventy‐five hospitalised patients who met the criteria of primary bilateral CRSwNP were enrolled from March 2020 to January 2021.Main outcome measuresVisual analogue scale, 22‐item Sino‐Nasal Outcome Test, Lund–Kennedy (L‐K) system and scanning electron microscopy and quantitative real‐time polymerase chain reaction.ResultsSeventy‐five patients with CRSwNP were randomly assigned to three groups, and 72 patients completed the 1‐month medication regimen and 1‐year follow‐up. Rh‐bFGF nasal‐spray and drop application reduced general nasal VAS scores within 2 weeks after endoscopic sinus surgery (ESS) compared to the control group. In contrast, only rh‐bFGF nasal‐drops reduced SNOT‐22 scores at 2 weeks and 1 year compared with the control group. A significant reduction in the endoscopic L‐K score was observed in the rh‐bFGF nasal‐spray and drop group compared with the control group. This is primarily because rh‐bFGF promotes cilia growth in the nasal mucosal epithelium after the operation, as illustrated by scanning electron microscopy and expression of CP110, Tap73 and Foxj1 mRNA. For eosinophilic CRSwNP, the general VAS score of rh‐bFGF nasal‐drops was more obviously reduced compared to the control group after ESS. A similar trend was observed for L‐K score.ConclusionsRh‐bFGF nasal‐drops and sprays can quickly and effectively relieve postoperative symptoms and improve long‐term prognosis of patients with CRSwNP. Moreover, rh‐bFGF nasal‐drops is also an effective method for postoperative patients with eosinophilic CRSwNP.
Tetrahedral framework nucleic acids‐based delivery promotes intracellular transfer of healing peptides and accelerates diabetic would healing
Authors: Lin Shiyu , Zhang Qi , Li Songhang , Qin Xin , Cai Xiaoxiao , Wang Huiming 
Published: July 2022

AbstractObjectivesPeptide‐based therapeutics are natural candidates to desirable wound healing. However, enzymatic surroundings largely limit its stability and bioavailability. Here, we developed a tetrahedral framework nucleic acids(tFNA)‐based peptide delivery system, that is, p@tFNAs, to address deficiencies of healing peptide stability and intracellular delivery in diabetic wound healing.Materials and MethodsAGEs (advanced glycation end products) were used to treat endothelial cell to simulate cell injury in diabetic microenvironment. The effects and related mechanisms of p@tFNAs on endothelial cell proliferation, migration, angiogenesis and ROS (reactive oxygen species) production have been comprehensively studied. The wound healing model in diabetic mice was photographically and histologically investigated in vivo.ResultsEfficient delivery of healing peptide by the framework(tFNA) was verified. p@tFNAs helped overcome the angiogenic obstacles induced by AGEs via ERK1/2 phosphorylation. In the meantime, p@tFNA exhibited its antioxidative property to achieve ROS balance. As a result, p@tFNA improved angiogenesis and diabetic wound healing in vitro and in vivo.ConclusionsOur findings demonstrate that p@tFNA could be a novel therapeutic strategy for diabetic wound healing. Moreover, a new method for intracellular delivery of peptides was also constructed.
Neutralizing antibody responses against SARS‐CoV‐2 in patients with plasma cell disorders who are on active treatment after two doses of mRNA vaccination
Authors: Abdallah Al‐Ola , Mahmoudjafari Zahra , Atieh Tahani , Ahmed Nausheen , Cui Wei , Shune Leyla , Mohan Meera , McGuirk Joseph , Remker Cassie , Foss Margaret , Karloff Ellie , Fitch Heather , Atrash Shebli 
Published: July 2022

AbstractMany patients with plasma cell disorder (PCD) on active treatment with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) require hospitalization, with an increased mortality rate over healthy adults. The FDA approved two mRNA vaccines against SARS‐CoV‐2: BNT162b2 and mRNA‐1273. To assess the efficacy of vaccination in patients with PCD, retrospectively, we identified all patients on active treatment. A total of 149 patients were included. Neutralizing antibodies (NAbs) levels against SARS‐CoV‐2 adequate, intermediate, and no response were observed in 42%, 32%, and 26%, respectively. Low NAbs were seen in patients on daratumumab combinations or anti‐BCMA therapy, low lymphocytes, and low IgG levels. Twenty‐three (15%) patients have SARS CoV‐2, while 8% required hospitalization, majority of these patients had intermediate or no response based on NAbs levels. Therefore, checking NAbs may be clinically helpful in identifying patients' responses. Further prospective studies should ascertain the value of a third vaccine dose in this population.
A retrospective study of treosulfan versus busulfan‐based conditioning in pediatric patients
Authors: Olivas‐Mazón Raquel , Bueno David , Sisinni Luisa , Mozo Yasmina , Casado‐Abad Gema , Martínez Antonio Pérez 
Published: July 2022

AbstractObjectivesTo compare the outcomes of treosulfan‐based vs busulfan‐based conditioning regimens in allogeneic hematopoietic stem cell transplantation (HSCT) in pediatric patients.MethodsRetrospective study of all consecutive patients (2012–2019) treated with allogenic HSCT and treosulfan‐ or busulfan‐based conditioning regimens at a single center.ResultsA total of 101 HSCT were included: 66 HSCT with busulfan and 35 with treosulfan. In malignant diseases (n = 62), busulfan‐based conditioning was more commonly employed than treosulfan: 82.3% vs 17.7%. However, the use of treosulfan for malignant diseases increased over time: 6.5% of HSCT in 2012–2015 vs 29% of HSCT in 2015–2019 (p = .02). The cohort of treosulfan had more children under 1‐year of age than the busulfan cohort (31 vs 13%; p = .033). The percentage of patients who received serotherapy was 73 and 89% in the nonmalignant and malignant groups, respectively. The engraftment, time to neutrophil, and platelet engraftment were not significantly different between the busulfan and the treosulfan cohorts. Rate of grade II‐IV acute GvHD was significantly higher in the busulfan cohort than the treosulfan cohort (39% vs 15%; p = .016). No differences were observed in endothelial damage complications, chronic GvHD, relapse, overall survival, and transplant‐related mortality.ConclusionsBusulfan‐based conditioning regimens are used more frequently for children undergoing allogenic HSCT, but treosulfan‐based conditioning is gaining acceptance. Treosulfan‐based conditioning is associated with lower rates of acute GvHD, and no significant differences on overall survival were observed compared with busulfan.
Software business process adaptive approach supporting organization architecture evolution
Authors: Li Youhuizi , Yin Yuyu , Li Yu , Hu Haijie , Lu Linyang , Cao Jie 
Published: July 2022

AbstractSoftware maintenance and evolution play an important role in the software engineering field, especially when current software becomes more and more complex and powerful. As an entity to implement business processes and gain revenue, valuable software is composed of business logic and corresponding organization role interaction interfaces. With the enterprise development, the organization architecture also evolves, like expanding, cross department cooperation, and so on. However, existing software process adaptive approaches mainly focus on handling the change of the business (program) logic instead of organization structure. Therefore, we propose an adaptive software business process approach that supports organization architecture evolution and automatically migrates the run‐time process instances to the latest version. First, a business process adaptation model is designed, which includes the organization layer, business process layer and event layer that connects the two. Based on the model, the organization changing impact and business process model modification are formalized. Besides, the business process adaptation approach is designed. According to the dependence between the organization architecture and the business process activities, the affected domain detection algorithms for three basic business process structures and the business process instance migration algorithm are developed. Finally, the feasibility and stability of the proposed system are comprehensively evaluated with the synthetic data sets.
Comparing growth and body condition of wild and hatcheryMYYbrook trout in streams and alpine lakes
Authors: Unsworth James S. , Roth Curtis J. , Meyer Kevin A. 
Published: July 2022

AbstractBiologists have theorized that stocking YY males (created via in‐hatchery hormonal sex‐reversal and selective breeding; hereafter MYYfish) could be used to eradicate unwanted non‐native vertebrate populations, but little is known about the fitness of MYYindividuals once released into the wild. We compared growth and body condition of stocked hatchery‐reared MYYbrook trout (Salvelinus fontinalisMitchell) to wild conspecifics in two streams and two alpine lakes. Maximum age for wild fish was age 6 at one stream and age 4 or 5 at the remaining waters, whereas for hatchery MYYfish, maximum age was age 5 at one stream and age 4 at the remaining waters. Total length ranged from 103 to 359 mm for wild brook trout and 115 to 353 mm for hatchery MYYbrook trout. Growth rates and body condition of stocked MYYbrook trout did not differ from wild fish in the same waters. Given that the success of MYYeradication programs is primarily contingent upon MYYindividuals having fitness characteristics similar to wild conspecifics, our results provide further evidence that the stocking of hatchery‐reared MYYfish may be a viable tool for eradicating unwanted non‐native fish populations.
(Hf0.25Zr0.25Sn0.25Ti0.25)O2 high‐entropy ceramics and their microwave dielectric characteristics
Authors: Ding Yi Han , Liu Lu , Guo Rui Ze , Li Lei , Chen Xiang Ming 
Published: July 2022

Abstract(Hf0.25Zr0.25Sn0.25Ti0.25)O2 high‐entropy ceramics were designed and prepared, and their microwave dielectric characteristics were investigated together with the microstructures. The single‐phase structure in space group Pbcn was determined, and the excellent microwave dielectric characteristics were achieved in the present ceramics: εr = 25.6, Qf = 74 600 GHz at 6.5 GHz and τf = −47 ppm/°C. The significant improvement of Qf value was achieved with a prolonged sintering, where the dielectric constant increased slightly while the negative τf decreased in the absolute value at first and then turned to increase. The variation tendency of microwave dielectric characteristics was deeply linked with the microstructure homogeneity, and the introduction of Hf and Sn elements to create the high‐entropy ceramics resulted in the disordered state, and finally led to the improved Qf value. The present work provided an effective approach to achieve enhanced Qf value in microwave dielectric ceramics.
Construction of miRNA‐lncRNA‐mRNA co‐expression network affecting EMT‐mediated cisplatin resistance in ovarian cancer
Authors: Naghsh‐Nilchi Amirhosein , Ebrahimi Ghahnavieh Laleh , Dehghanian Fariba 
Published: July 2022

AbstractPlatinum resistance is one of the major concerns in ovarian cancer treatment. Recent evidence shows the critical role of epithelial–mesenchymal transition (EMT) in this resistance. Epithelial‐like ovarian cancer cells show decreased sensitivity to cisplatin after cisplatin treatment. Our study prospected the association between epithelial phenotype and response to cisplatin in ovarian cancer. Microarray dataset GSE47856 was acquired from the GEO database. After identifying differentially expressed genes (DEGs) between epithelial‐like and mesenchymal‐like cells, the module identification analysis was performed using weighted gene co‐expression network analysis (WGCNA). The gene ontology (GO) and pathway analyses of the most considerable modules were performed. The protein–protein interaction network was also constructed. The hub genes were specified using Cytoscape plugins MCODE and cytoHubba, followed by the survival analysis and data validation. Finally, the co‐expression of miRNA‐lncRNA‐TF with the hub genes was reconstructed. The co‐expression network analysis suggests 20 modules relating to the Epithelial phenotype. The antiquewhite4, brown and darkmagenta modules are the most significant non‐preserved modules in the Epithelial phenotype and contain the most differentially expressed genes. GO, and KEGG pathway enrichment analyses on these modules divulge that these genes were primarily enriched in the focal adhesion, DNA replication pathways and stress response processes. ROC curve and overall survival rate analysis show that the co‐expression pattern of the brown module's hub genes could be a potential prognostic biomarker for ovarian cancer cisplatin resistance.
Sildenafil improves radiation‐induced oral mucositis by attenuating oxidative stress, NF‐κB, ERK and JNK signalling pathways
Authors: Ala Moein , Mohammad Jafari Razieh , Ala Mahan , Hejazi Sedigheh Marjaneh , Tavangar Seyed Mohammad , Mahdavi Seied Rabi , Dehpour Ahmad Reza 
Published: July 2022

AbstractRadiation‐induced oral mucositis is a common and dose‐limiting complication of head and neck radiotherapy with no effective treatment. Previous studies revealed that sildenafil, a phosphodiesterase 5 inhibitor, has anti‐inflammatory and anti‐cancer effects. In this study, we investigated the effect of sildenafil on radiation‐induced mucositis in rats. Two doses of radiation (8 and 26 Gy X‐ray) were used to induce low‐grade and high‐grade oral mucositis, separately. A control group and three groups of sildenafil citrate‐treated rats (5, 10, and 40 mg/kg/day) were used for each dose of radiation. Radiation increased MDA and activated NF‐κB, ERK and JNK signalling pathways. Sildenafil significantly decreased MDA level, nitric oxide (NO) level, IL1β, IL6 and TNF‐α. The most effective dose of sildenafil was 40 mg/kg/day in this study. Sildenafil also significantly inhibited NF‐κB, ERK and JNK signalling pathways and increased bcl2/bax ratio. In addition, high‐dose radiation severely destructed the mucosal layer in histopathology and led to mucosal cell apoptosis in the TUNEL assay. Sildenafil significantly improved mucosal structure and decreased inflammatory cell infiltration after exposure to high‐dose radiation and reduced apoptosis in the TUNEL assay. These findings show that sildenafil can improve radiation‐induced oral mucositis and decrease the apoptosis of mucosal cells via attenuation of inflammation and oxidative stress.
Impact of a leaf‐cutting ant (Atta sexdens L.) on a Pinus taeda plantation: A 6 year‐long study
Authors: Scherf Abel Nicolas , Corley Juan Carlos , Gioia Carlos David , Eskiviski Edgar Rafael , Carazzo Cristhian , Patzer Hernán Raul , Dimarco Romina Daniela 
Published: July 2022

AbstractLeaf‐cutting ants are the most economically important pest of plantation forests in the Neotropics. These social insects can defoliate whole trees, reducing their growth ability and occasionally causing tree mortality. Control of leaf‐cutting ants in plantations is mainly carried out by using insecticides such as sulfluramid or fipronil. Foresters that adhere to voluntary sustainability certification systems are requested to implement integrated pest management programmes that reduce the use of insecticides. However, this approach is much delayed since it requires a better understanding of ant ecology, including quantitative data on the long‐term impact that leaf‐cutting ants have on tree plantations. In this study, we show, for the first time, the results on the long‐term impact of Atta sexdens L. on a Pinus taeda L. plantation in NE Argentina. The annual growth in diameter, height, volume and mortality of P. taeda trees in two 11 ha stands were evaluated up to the middle of the harvest cycle (i.e. 6 years). One of the stands had no control of A. sexdens nests, and in the other stand, all ant nests were removed (total control). After six years since planting, P. taeda trees show significant reductions in total height (19%), diameter at breast height (4.4%) and wood volume (21%) in sites with A. sexdens present. Moreover, tree mortality reached 31.2% when ants were present. Forest managers could use these results to improve their integrated management programmes, by better adjusting their economic damage threshold parameters.
Presence and distribution of the African citrus psyllid in São Tomé island
Authors: Benhadi‐Marín Jacinto , Félix‐Oliveira Diogo , Carvalho Miclay Dos Reis Pereira , Mendes José Luis , Baptista Paula , Pereira José A. 
Published: July 2022

AbstractThe African citrus psyllid, Trioza erytreae (Hemiptera: Triozidae), is a natural vector of Candidatus Liberibacter spp., the causal agents of the citrus greening disease or Huanglongbing (HLB). Despite the global losses of the Citrus industry due to HLB, the vector is rapidly spread to new areas threatening citrus crops and raising concern for stakeholders. Until now, there was a single old register of T. erytreae from the São Tomé island (Bulletin of the British Museum of Natural History, Entomology, 49, 1–102). In this work, we provide up‐to‐date occurrence data on the presence of T. erytreae throughout the isle of São Tomé. The identity of the captured adults was assessed and confirmed through molecular identification by sequencing a portion of the mitochondrial COI. The psyllid was found in lemon, orange and tangerine orchards. Further monitoring is advisable to manage potential outbreaks.
Growth, consumption and digestive enzyme activities of Spodoptera littoralis (Boisd) on various mung bean cultivars reveal potential tolerance traits
Authors: Zamani Fard Shabnam , Hemmati Seyed Ali , Shishehbor Parviz , Stelinski Lukasz L. 
Published: July 2022

AbstractSpodoptera littoralis (Boisd) is a worldwide pest of economic importance for agricultural crops. We investigated nutritional efficiency and digestive enzyme activity of S. littoralis on various mung bean cultivars, including Parto, Simite1, Simite2, VC6173A, VC3960, VC6368 and VC6371. Furthermore, various biochemical traits of mung bean cultivars, including protein, starch, total phenolic and flavonoid content, were measured to investigate their relationships with nutritional physiology of S. littoralis. The nutritional indices, as well as proteolytic and amylolytic activities of S. littoralis were significantly affected by feeding of the pest on various mung bean cultivars. Larvae reared on VC6371 exhibited the highest efficiency of conversion of digested food, while the lowest was recorded in those fed on Simite2. Spodoptera littoralis larvae fed on the Simite1 cultivar exhibited the lowest relative growth rate, while those reared on Simite2 were characterized by the lowest efficiency of conversion of ingested food. The highest proteolytic activity of sixth‐instar larvae was on VC6371, while the lowest activity was on VC3960, Simite1 and Simite2 cultivars. Amylolytic activity was the lowest in those larvae fed on VC6368 and VC6371 cultivars, while the highest activity occurred in larvae reared on Simite2. Significant variation in phytochemical contents was quantified among tested mung bean cultivars. Moreover, significant correlations were found between the nutritional indices and digestive enzyme activities of S. littoralis with biochemical properties of the cultivars. Our results suggest that VC6371 is a suitable cultivar for S littoralis, while Simite2 and Simite1 cultivars are less suitable and may be useful selections to minimize damage caused by this pest.
How innovations in methodology offer new prospects for volume electron microscopy
Authors: Kievits Arent J. , Lane Ryan , Carroll Elizabeth C. , Hoogenboom Jacob P. 
Published: July 2022

AbstractDetailed knowledge of biological structure has been key in understanding biology at several levels of organisation, from organs to cells and proteins. Volume electron microscopy (volume EM) provides high resolution 3D structural information about tissues on the nanometre scale. However, the throughput rate of conventional electron microscopes has limited the volume size and number of samples that can be imaged. Recent improvements in methodology are currently driving a revolution in volume EM, making possible the structural imaging of whole organs and small organisms. In turn, these recent developments in image acquisition have created or stressed bottlenecks in other parts of the pipeline, like sample preparation, image analysis and data management. While the progress in image analysis is stunning due to the advent of automatic segmentation and server‐based annotation tools, several challenges remain. Here we discuss recent trends in volume EM, emerging methods for increasing throughput and implications for sample preparation, image analysis and data management.
Comparison of three different surgical approaches for the treatment of large/giant congenital melanocytic nevus
Authors: Cheng Xialin , Wang Wenxuan , He Yan , Bao Wu , Jia Chiyu , Dai Tao 
Published: July 2022

AbstractObjectiveTreating large/giant congenital melanocytic nevus (L/GCMN) is challenging for surgeons. Operative approaches commonly used to remove L/GCMN include serial excision, tissue expansion, and skin grafting. Thus, we retrospectively compared these three operations' applications and therapeutic effects.MethodsThe clinical data of 97 L/GCMN patients from June 1, 2015, to June 1, 2019, were collected and divided into three groups according to the operations used: serial excision group (SE group, n = 18), tissue expansion group (TE group, n = 23), and skin grafting group (SG group, n = 56). The location and size of the lesion, the number of operations, duration of each operation, preoperative preparation time, postoperative hospital stay, complications, and clinical outcomes of all patients were collected and assessed.ResultsThe SE group had the most times of operation (3.9 and 6.0 for LCMN and GCMN, respectively), the shortest surgery length (56.3 min), and the shortest postoperative hospital stay (10.0d). The SE and SG groups required much less time to prepare for surgery and had a lower rate of complications than the TE group. During the 11.9‐month median follow‐up period, the SE and TE groups had better postoperative outcomes than the SG group.ConclusionEach of the three operations has different advantages and disadvantages, and the specific surgical strategy should be decided based on the patient's unique circumstances.