bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adverse Childhood Experiences and Their Impact on Sleep in Adults
Authors: Vadukapuram Ramu , Shah Kaushal , Ashraf Sahar , Srinivas Sushma , Elshokiry Amir Bishay , Trivedi Chintan , Mansuri Zeeshan , Jain Shailesh 
Published: May 2022

Abstract
Adverse childhood experiences (ACEs) before the age of 18 years are pervasive and noteworthy public health concerns. The ACEs are associated with sleep disorders in later life. In this study, we conduct a systematic review to explore the effects of ACEs on sleep in adulthood. Using Medical Subject Headings keywords, we searched Medline, PubMed, PubMed Central, the American Psychological Association PsycArticles, and PsychInfo databases to evaluate the association between ACEs and sleep disturbances. ACEs increase the odds of developing chronic short sleep duration, that is, <6 hours of sleep per night compared with optimal sleep duration of 7–9 hours per night during adulthood. The ACEs are positively associated with poor sleep characteristics such as short sleep duration and long-term sleep problems. Clinicians should pay close attention to developmental trauma care, access community health programs, and help develop better coping skills, resiliency, and good sleep habits in their patients.
The How and the Why of Including Children: Experiential Learning in Teaching Physical Therapist Students
Authors: Wynarczuk Kimberly D. , Gagnon Kendra , Fiss Alyssa LaForme , Kendall Eydie , Schreiber Joseph , Rapport Mary Jane 
Published: May 2022

Purpose:
This study describes experiential learning (EL) activities with children and the rationale for using EL. Experiential learning with children in entry-level doctor of physical therapy (DPT) education has not been described.

Methods:
Eighteen pediatric educators from accredited DPT programs participated in semistructured, in-depth interviews. Researchers analyzed written interview transcriptions using an iterative, consensus-building, thematic analysis approach.

Results:
Educators described their EL activities. Educators' rationale for using EL grouped around 4 themes: (1) EL is valued by multiple stakeholders, (2) EL provides an additional learning modality, (3) EL introduces students to pediatric physical therapy, and (4) EL addresses standards and competencies for DPT education.

Conclusions:
This research provides a rich description of EL with children and rationale for integrating EL in DPT education. These findings may inform educators and program directors regarding the value of EL and the investment of resources to support EL in DPT education.

Supplemental Video Abstract available
at: http://links.lww.com/PPT/A386
Volumetric Assessment of Hippocampus and Subcortical Gray Matter Regions in Alzheimer Disease and Amnestic Mild Cognitive Impairment
Authors: Topkan Tuğberk Andaç , Erdogan Nesrin , Barutcu Berrak , Cindil Emetullah , Tali E. Turgut , Karaman Yahya 
Published: May 2022

Background:
Quantitative MRI assessment methods have limited utility due to a lack of standardized methods and measures for Alzheimer disease (AD) and amnestic mild cognitive impairment (aMCI).

Objective:
To employ a relatively new and easy-to-use quantitative assessment method to reveal volumetric changes in subcortical gray matter (GM) regions, hippocampus, and global intracranial structures as well as the diagnostic performance and best thresholds of total hippocampal volumetry in individuals with AD and those with aMCI.

Method:
A total of 74 individuals—37 with mild to moderate AD, 19 with aMCI, and 18 with normal cognition (NC)—underwent a 3T MRI. Fully automated segmentation and volumetric measurements were performed.

Results:
The AD and aMCI groups had smaller volumes of amygdala, nucleus accumbens, and hippocampus compared with the NC group. These same two groups had significantly smaller total white matter volume than the NC group. The AD group had smaller total GM volume compared with the aMCI and NC groups. The thalamus in the AD group showed a subtle atrophy. There were no significant volumetric differences in the caudate nucleus, putamen, or globus pallidus between the groups.

Conclusion:
The amygdala and nucleus accumbens showed atrophy comparable to the hippocampal atrophy in both the AD and aMCI groups, which may contribute to cognitive impairment. Hippocampal volumetry is a reliable tool for differentiating between AD and NC groups but has substantially less power in differentiating between AD and aMCI groups. The loss of total GM volume differentiates AD from aMCI and NC.
Pseudophaeobacter flagellatus sp. nov., isolated from coastal water
Authors: Guan Yong , Jiang Yue , Kim Young-Min , Yu Seung Yeob , Choi Seung-Hyeon , Choe Hanna , Li Zhun , Lee Mi-Kyung 
Published: May 2022

A Gram-stain-negative, aerobic, motile, rod-shaped novel bacterial strain, designated as MA21411-1T, was isolated from the Korean coast. The colonies were white-yellow-coloured, smooth, convex and entire, spherical and 1.0–1.8 mm in diameter. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain MA21411-1T is closely related to species of the genus

Pseudophaeobacter

. The 16S rRNA gene sequence similarities between strain MA21411-1T and

Pseudophaeobacter arcticus

DSM 23566T,

Phaeobacter porticola

DSM 103148T and

Pseudophaeobacter leonis

DSM 25627T were 98.31, 97.80 and 97.28 %, respectively. Strain MA21411-1T has a draft genome size of 4 294 042 bp, annotated with 4125 protein-coding genes, and 53 tRNA, three rRNA and one tmRNA genes. The genomic DNA G+C content was 59.2 mol%. Comparative genome analysis revealed that the average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values among strain MA21411-1T and other related species were below the cut-off levels of 95, 70 and 95.5 %, respectively. The growth temperature range for growth was 15–28 °C (optimum, 25 °C), pH range was 6.0–9.0 (optimum, pH 6.0), and salt tolerance range was 0.5–4 % (optimum 0.5 %). Ubiquinone-10 was the sole quinone present in MA21411-1T and all three closely related strains. The major cellular fatty acid (>10 %) of the strain was summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c). The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and four unidentified polar lipids. Based on the phylogenetic tree, as well as phenotypic, chemotaxonomic and genomic features, strain MA21411-1T represents a novel species of the genus

Pseudophaeobacter

, for which the name Pseudophaeobacter flagellatus sp. nov. is proposed. The type strain is MA21411-1T (=KCTC 92095T=GDMCC 1.2988T).
Accelerating single-cell genomic analysis with GPUs
Authors: Nolet Corey , Lal Avantika , Ilango Rajesh , Dyer Taurean , Movva Rajiv , Zedlewski John , Israeli Johnny 
Published: May 2022

AbstractSingle-cell genomic technologies are rapidly improving our understanding of cellular heterogeneity in biological systems. In recent years, technological and computational improvements have continuously increased the scale of single-cell experiments, and now allow for millions of cells to be analyzed in a single experiment. However, existing software tools for single-cell analysis do not scale well to such large datasets. RAPIDS is an open-source suite of Python libraries that use GPU computing to accelerate data science workflows. Here, we report the use of RAPIDS and GPU computing to accelerate single-cell genomic analysis workflows and present open-source examples that can be reused by the community.
Coupled Mutual Inhibition and Mutual activation motifs as tools for cell-fate control
Authors: Sabuwala Burhanuddin , Hari Kishore , Abhishek S V , Jolly Mohit Kumar 
Published: May 2022

AbstractMultistability is central to biological systems as it plays a key role in adaptation, evolvability, and differentiation. Multistability can be achieved by integrating positive feedback loops into the regulation. The simplest of such feedback loops are a) a mutual inhibition (MI) loop, b) a mutual activation (MA) loop, and, c) self-activation. While it is established that all three motifs can give rise to bistability, the characteristic differences in the bistability exhibited by each of these motifs is relatively less understood. Here, we use ODE-based simulations across a large ensemble of parameter sets and initial conditions to study the characteristics of the bistability of these motifs and their limits in the parameter space. We also examine the behavior of these motifs under mutual degradation and self-activation. Finally, we investigate the utility of these motifs for achieving coordinated expression through cyclic and parallel coupling. Through our analysis, we found that MI-based architectures offer robustness in maintaining distinct multistability and allow for coordination among multiple genes. This coordination paves way for understanding the naturally occurring gene regulatory network and may also help in the better design of robust and controllable synthetic networks.
Types of occupational accidents and their predictors at construction sites in Lusaka city
Authors: Simutenda P. , Zambwe M. , Mutemwa R. 
Published: May 2022

ABSTRACTObjectiveTo determine the types of occupational accidents and their predictors at construction sites in Lusaka city.MethodsA qualitative research design which explored the views, experiences, and beliefs of construction workers. A sample size of seven participants who were previously involved in at least one construction accident was drawn purposively. Data was collected by interviewing participants using a semi-structured interview schedule and a voice recorder. The data from the voice recorder was then transcribed in verbatim format. Thematic evaluation was used for data analysis.FindingsTwo themes were identified:- types of occupational accidents and predictors of accidents. The types of construction accidents included crane or hoist accidents, slips, and falls from heights, gas leaks, fires and explosions, forklift, trench, electrocutions, machinery, moving or failing object, caught-between and exposure to dangerous chemicals. Predictors of construction site accidents were mainly human factors and site conditions.ConclusionThe study established the types of accidents and their predictors The types of accidents established can cause immerse human suffering and even death. Therefore, effective accident prevention policies need to be devised, adhered to and continuously reviewed.
Investigation of a SARS-CoV-2 outbreak in a Texas summer camp resulting from a single introduction
Authors: Swetnam Daniele M. , Alvarado R. Elias. , Sotcheff Stephanea , Mitchell Brooke M. , McConnell Allan , Machado Rafael R.G. , Saada Nehad , Haseltine Florence P. , Maknojia Sara , Smith Anajane , Ren Ping , Keiser Philip , Weaver Scott C. , Routh Andrew 
Published: May 2022

AbstractSARS-CoV-2 is the etiological agent responsible for the COVID-19 pandemic. It is estimated that only 10 aerosol-borne virus particles are sufficient to establish a secondary infection with SARS-CoV-2. However, the dispersal pattern of SARS-CoV-2 is highly variable and only 10– 20% of cases are responsible for up 80% of secondary infections. The heterogeneous nature of SARS-CoV-2 transmission suggests that super-spreader events play an important role in viral transmission. Super-spreader events occur when a single person is responsible for an unusually high number of secondary infections due to a combination of biological, environmental, and/or behavioral factors. While super-spreader events have been identified as a significant factor driving SARS-CoV-2 transmission, epidemiologic studies have consistently shown that education settings do not play a major role in community transmission. However, an outbreak of SARS-CoV-2 was recently reported among 186 children (aged 10-17) and adults (aged 18 +) after attending an overnight summer camp in Texas in June 2021. To understand the transmission dynamics of the outbreak, RNA was isolated from 36 nasopharyngeal swabs collected from patients that attended the camp and 19 control patients with no known connection to the outbreak. Genome sequencing on the Oxford Nanopore platform was performed using the ARTIC approaches for library preparation and bioinformatic analysis. SARS-CoV-2 amplicons were produced from all RNA samples and >70% of the viral genome was successfully reconstructed with >10X coverage for 46 samples. Phylogenetic methods were used to estimate the transmission history and suggested that the outbreak was the result of a single introduction. We also found evidence for secondary transmission from campers to the community. Together, these findings demonstrate that super-spreader events may occur during large gatherings of children.
Subthreshold Nanosecond Laser Treatment of Intermediate AMD without Reticular Pseudodrusen: Real-world Three-year Follow-up Study
Authors: Worsley David R. , Louise Jennie , Bull Susan P. 
Published: May 2022

AbstractPurposeSubthreshold nanosecond laser (SNL) has been proposed to reduce the risk of intermediate age-related macular degeneration (iAMD) progressing to late AMD. The phase 3 LEAD Study post-hoc analysis indicates a potentially large benefit from SNL treatment of eyes without reticular pseudodrusen. This real-world study reports the three-year outcomes of SNL treatment of iAMD without RPD.MethodsAn observational retrospective single-centre cohort study of all patients with iAMD, centre-involving very large soft drusen (≥250 μm diameter) without RPD, treated with SNL and with three-year follow up. The primary outcome measured was progression to late AMD; neovascular AMD (nAMD) or geographic atrophy (GA).ResultsThere were 120 eyes of 64 patients. At baseline, the cohort had a high risk profile; drusen median area, volume and largest diameter were 0.70mm2(IQR: 0.20 to 1.50), 0.03mm3(IQR: 0.01 to 0.08) and 835μm (IQR: 446.50 to 1398.50) respectively; hyperreflective foci were present in 56.67%; and hyporeflective drusen cores in 25.83%. Eyes had a mean of 3.03 treatments. By three years, progression to late AMD occurred in 5.83% of eyes, all to GA. Visual acuity was stable or improved in 80% of eyes.ConclusionThe three-year progression rate was low compared with published 36-month natural histories of iAMD without RPD. The progression rate was similar to the LEAD study SNL-treated group of iAMD without RPD. This study supports the hypothesis that SNL for iAMD without RPD may reduce progression to late AMD. Further investigation is warranted.
A completeness indicator of gestational and congenital syphilis information in Brazil
Authors: de Oliveira Guilherme Lopes , Ferreira Andrêa JF , Guilherme Santana José , Lana Raquel Martins , Cardoso Andrey Moreira , Teles Carlos , Fiaccone Rosemeire L. , Aquino Rosana , Santos Soares Maria Auxiliadora , Paixão Enny S. , dos Santos Idália Oliveira , Salvi Leonardo , Barreto Mauricio L. , Ichihara Maria Yury 
Published: May 2022

AbstractObjectiveTo evaluate the quality of information on gestational syphilis (GS) and congenital syphilis (CS) on the Notifiable Diseases Information System (SINAN-Syphilis Brazil) by compiling and validating completeness indicators between 2007 and 2018.Materials and methodsOverall, care and socioeconomic completeness scores were compiled based on selected variables, using weights assigned by specialists. The completeness scores were analysed, considering the region and area of residence, pregnant race/colour, and year of case notification. Pearson’s correlation coefficients were used to validate the scores obtained by the weighted average method, compared with the values obtained through principal component analysis (PCA).ResultMost of the variables selected presented a good or excellent degree of completeness for GS and CS, except for clinical classification, pregnant woman’s level of education, partner’s treatment, and child’s race/colour, which were classified as poor or very poor. The overall (89.93% versus 89.69%) and socioeconomic (88.71% versus 88.24%) completeness scores for GS and CS, respectively, were classified as regular, while the care score (GS-90, 88%, and CS-90, 72%) was good, although there were improvements over time. Differences in the overall, care and socioeconomic completeness scores according to region, area of residence, and ethnic-racial groups were reported for syphilis notifications. A strong linear correlation (>0.90) was observed between the completeness scores estimated through the weighted average method and PCA.ConclusionImprovements in the completeness of GS and CS notifications have been observed in recent years, highlighting the variables which form the care score, compared to the socioeconomic scores, despite differences between regions, area of residence, and ethnic-racial groups. The weighted average was a viable methodological alternative easily operationalised to estimate data completeness scores, allowing routine monitoring of the completeness of gestational and congenital syphilis records.
Specifying uniform eligibility criteria to strengthen causal inference studies of long-term outcomes of COVID-19
Authors: Erqou Sebhat , Zullo Andrew R , Jiang Lan , Khetpal Vishal , Berkowitz Julia , Shah Nishant R. , Echouffo-Tcheugui Justin B. , Rudolph James L. , Choudhary Gaurav , Wu Wen-Chih 
Published: May 2022

AbstractBackgroundCausal interpretation of findings from existing epidemiological studies on long-term clinical outcomes of coronavirus disease 2019 (COVID-19) may be limited by the choice of comparator (control) group.ObjectiveWe compare two approaches to control group selection (based on requirement for negative SARS-CoV-2 test for eligibility) in long-term clinical outcomes after COVID-19 in patients with history of heart failure (HF).DesignRetrospective cohort study using data from February 1, 2020 to July 31, 2021. Setting: Veteran Health Administration (VHA).ParticipantsWe studied two cohorts of Veterans with COVID-19 and history of HF which selected comparison group using two different approaches. In Cohort I, Veterans with HF who tested for positive for SARS-CoV-2 were age, sex, and race matched to Veterans with no evidence of COVID-19 in 1:5 ratio. In Cohort II Veterans with HF who tested positive for SARS-CoV-2 were age, sex, and race matched with Veterans with HF who tested negative for SARS-CoV-2 within +/-15 days of the positive test date within the same VHA facility.ExposureCOVID-19 as determined by a positive SARS-CoV-2 test.Main Outcomes and Measures1-year all-cause mortality and hospital admissions beyond the first 30 days after COVID-19 diagnosis. Adjusted hazard ratios (HRs) accounting for comorbidity and 95% confidence intervals were calculated.ResultsCohort I comprised 13,722 Veterans with HF with COVID-19 (mean [SD] age 72.0 [10.2] years, 2.4% female, 71.1% White) and 60,956 matched controls not known to have COVID-19. Cohort II comprised 6,725 Veterans with HF with COVID-19 (mean [SD] age 72.5 [7.5] years, 0.1% female, 80.8% White) and 6,726 matched controls with negative SARS-CoV-2 test. The adjusted HRs for 1-year mortality and hospital admission beyond the first 30 days after diagnosis of COVID-19 were 1.40 (1.32-1.49) and 1.34 (1.28-1.41), respectively, in analysis of Cohort-I (where the comparator group was not required to test negative for SARS-CoV-2). However, in Cohort-II (using the second comparator group specifying negative SARS-CoV-2 test for eligibility), the associations were markedly attenuated; adjusted HRs 1.05 (0.95-1.17) and 1.07 (0.96-1.19), respectively.ConclusionsWe found significant attenuation of associations between COVID-19 and long-term risk of mortality and hospital admissions beyond the first 30 days among patient with existing HF, when comparing with a control group selected based on a negative SARS-CoV-2 test versus control group not known to have COVID-19. The findings have implications for the design of studies of long-term CVD (and non-CVD) outcome of COVID-19.
Adverse PFAS effects on mouse oocyte in vitro maturation are associated with carbon-chain length and inclusion of a sulfonate group
Authors: Feng Jianan , Soto-Moreno Edgar J. , Prakash Aashna , Balboula Ahmed Z. , Qiao Huanyu 
Published: May 2022

AbstractPer- and polyfluoroalkyl substances (PFAS) are man-made chemicals that are used in products such as non-stick cookware, stain-resistant coating, and food packaging. PFAS are characterized by their fluorinated carbon chains that make them hard to degrade and bioaccumulate in human and animals. Toxicological studies have shown PFAS toxic effects: cytotoxicity, immunotoxicity, neurotoxicity, and reproductive toxicity. Two major categories of PFAS are perfluoroalkyl carboxylic acid (PFCA) and perfluoroalkyl sulfonic acid (PFSA). In this study, we used a mouse-oocyte-in-vitro-maturation (IVM) system to study how the structures of PFAS, such as carbon-chain length and functional groups, determine their reproductive toxicity. We found the toxicity of PFAS is elevated with increasing carbon-chain length and the inclusion of the sulfonate group. Specifically, at 600 µM, perfluorohexanesulfonic acid (PFHxS) and perfluorooctanesulfonic acid (PFOS) reduced the rates of both germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) as well as induced the formation of relatively large polar bodies. However, the shorter PFSA, perfluorobutanesulfonic acid (PFBS), and all PFCA did not show similar adverse cytotoxicity. We further examined mitochondria and cytoskeleton, two essential factors for cell division, in PFOS- and PFHxS-treated oocytes. We found that 600 µM PFHxS and PFOS exposure induced excess reactive oxygen species (ROS) and decreased mitochondrial membrane potential (MMP). Cytoskeleton analysis revealed that PFHxS and PFOS exposure induced chromosome misalignment, abnormal F-actin organization, elongated the spindle formation, and symmetric division in the treated oocytes. Together, our study provides new information on the structure-toxicity relationship of PFAS.SynopsisReproductive toxicity of PFAS, a group of persistent organic pollutants, is determined by their chemical structures.
The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
Authors: Navarro-Quiles Carla , Mateo-Bonmatí Eduardo , Candela Héctor , Robles Pedro , Martínez-Laborda Antonio , Fernández Yolanda , Šimura Jan , Ljung Karin , Rubio Vicente , Ponce María Rosa , Micol José Luis 
Published: May 2022

SUMMARYATP-Binding Cassette E (ABCE) proteins dissociate cytoplasmic ribosomes after translation terminates, and contribute to ribosome recycling, thus linking translation termination to initiation. This function has been demonstrated to be essential in animals, fungi, and archaea, but remains unexplored in plants.In most species, ABCE is encoded by a single-copy gene; by contrast,Arabidopsis thalianahas twoABCEparalogs, of whichABCE2seems to conserve the ancestral function. We isolatedapiculata7-1 (api7-1), a viable, hypomorphic allele ofABCE2,which has a pleiotropic morphological phenotype reminiscent of mutations affecting ribosome biogenesis factors and ribosomal proteins. We also studiedapi7-2, a null, recessive lethal allele ofABCE2.Co-immunoprecipitation experiments showed that ABCE2 physically interacts with components of the translation machinery. An RNA-seq study of theapi7-1mutant showed increased responses to iron and sulfur starvation. We also found increased transcript levels of genes related to auxin signaling and metabolism.Our results support a conserved role for ABCE proteins in ribosome recycling in plants, as previously shown for the animal, fungal, and archaeal lineages. In plants, the ABCE2 protein seems important for general growth and vascular development, likely due to an indirect effect through auxin metabolism.