bluuebunny/crossref_metadata_2025_split huggingface dataset:
Delay of switch from hypo- to hyperperfusion in migraine aura evidenced by arterial spin labeling (ASL): case series and pooled-data analysis
Authors: Benzakoun Joseph , Bouc Raphaël Le , Law-ye Bruno 
Published: February 2022

Abstract
Introduction: Arterial Spin Labeling (ASL) is an MRI technique used to non-invasively assess cerebral blood flow in clinical practice. In migraine aura, previous studies have shown both cortical hypoperfusion and hyperperfusion patterns. Sequential perfusion changes with alternation of hypoperfusion and hyperperfusion has been scarcely reported, only in children. Our goal was to determine the delay of conversion between hypoperfusion and hyperperfusion at acute phase, using ASL technique, in adult patients presenting migrainous aura.Materials and methods: Six local patients with migrainous aura presenting as stroke mimics were explored with ASL sequence. Two had repeated ASL exploration. Literature search allowed to include 9 additional patients. Mann-Whitney-U test, logistic regression and ROC curve analysis were conducted on pooled data with a total of 15 patients.Results: Median age was 30 years (range 17-87). Hypoperfusion was present on 7 ASL acquisitions and hyperperfusion on 10. Median onset-to-MRI delay was higher in hyperperfused pattern group than in hypoperfused pattern group (17.6 vs 3.5 hours, p<0.001). Best cut-off for separating hypoperfused and hyperperfused patterns was 4.5 hrs (95% confidence interval: 3.75-8.5-hrs).Conclusion: Cerebral blood perfusion pattern in ASL switches from hypoperfusion to hyperperfusion 4.5 hours after symptom onset time in adult population. Repeated ASL exploration is an asset for the differential diagnosis between stroke and migraine.
The Use of Borderline Personality Disorder Severity Index-IV Feedback in Adjusting Borderline Personality Disorder Treatment: Therapists and Patients Perspectives
Authors: de Wilde Odette Brand - , Clarke Sharon , Arntz Arnoud 
Published: February 2022

Abstract
Background: Previous research has emphasized the importance of therapists giving Routine Outcome Monitoring (ROM) feedback to their patients. It has been shown that several factors influence therapists’ tendency to provide ROM feedback to their patients.Methods: In this qualitative study, using a semi-structured interview followed by thematic analysis, we focused on experiences of therapists and patients with a disorder specific ROM instrument: the Borderline Personality Disorder Index-IV (BPDSI-IV). Ten patients with a borderline personality disorder who had been in Mentalization Based Treatment (MBT) and ten MBT-therapists treating patients with a borderline personality disorder were interviewed. Qualitative analysis in Atlas.ti revealed that patients experienced benefits of ROM using the BPDSI-IV.Results: Patients gained more insight in and recognition of their borderline personality disorder symptoms. They also felt more understood by the therapist because they got an opportunity to explain their symptoms in a different way than in a regular therapy session. Therapists shared they didn’t always use all the ROM outcomes as serious feedback for adjusting treatment. They preferred to use the BPDSI-IV over the other ROM instruments, because the BPDSI-IV is disorder specific, which gives insight into the treatment course of the patient. Conclusions: Experiences of both patients and therapists with the BPDSI-IV were positive. It seems to be valuable and promising for healthcare institutions to evaluate treatment with a disorder specific ROM instrument. Trial registration: The study was approved by the ethical committee of the University of Amsterdam (number 2017-CP-8000).
Study on the vertical release characteristics of alkali and fluoride in red mud with different compaction degrees
Authors: Ou Xiaoduo , chen shengjin , Jiang Jie , Qin Jinxi , Tan Zhijie , Su Wei 
Published: February 2022

Abstract
In this study, the vertical migration and release characteristics of alkali and fluoride in Bayer red mud with different compaction degrees were investigated by conducting constant head vertical leaching tests. The experimental results showed that the breakthrough time of leaching liquid across the red mud column increased with the increase of compaction degree, the alkali and fluoride concentrations of the leaching outflow decreased fast as the compaction degree of red mud column increased, as well as the time required to reach the leaching equilibrium. Moreover, for all the leaching red mud columns, the cumulative release of alkali and fluorine from the increased with the increase of leaching durations. However, the total release of major pollutants decreased with the increase of red mud compactness. After leaching, the soluble alkali and water-soluble fluorine (Ws-F) residual in the red mud gradually increased from top to bottom along the leaching column, and at the identical depth, the higher the compactness of the leaching column, the greater the soluble alkali and water-soluble fluorine (Ws-F) remaining in the column after leaching. These phenomena indicated that improving the compactness can effectively slow down the release of main pollutants in the red mud. The results were expected to provide a scientific basis for the prevention and control of alkali and fluoride pollutants in red mud yard.
Psychometric Properties Of Medical Professionals Resilience Scale (Mers) For Medical Doctors In Pakistan
Authors: Sohail Marium , Yusoff Muhamad Saiful Bahri Bin , Hadie Siti Nurma Hanim Bt , Othman Azizah Binti , Rauf Haris 
Published: February 2022

Abstract
Background: Medical Professionals Resilience Scale (MeRS) is a four-point Likert scale for measuring resilience in the domains of control, resourcefulness, involvement, and growth. This 37-item inventory was previously validated among Malaysian health professionals. To increase the evidence of validity of MeRS, this study attempted to cross validate and perform psychometric analysis of MeRS among medical doctors of Pakistan. Methods: A cross sectional study was conducted in two different hospitals of Pakistan. Prior to the study, ethical approval was obtained from the ERC/IRB committee of SKBZAN/AK CMH Rawalakot. The MeRS was distributed to 201 medical doctors, comprising of 102 house officers and 99 medical officers. Data was analyzed for descriptive statistics, reliability analysis and confirmatory factor analysis using the Statistical Package for the Social Sciences software version 26, and the Analysis of a Moment Structures software version 26 respectively. Results: The four-domain, 37-item MeRS has a good internal consistency with Cronbach’s alpha value of 0.90. The reliability of growth, control, involvement and resourceful domains are 0.76, 0.86, 0.79 and 0.79 having 15, 6, 12 and 4 items respectively. The confirmatory factor analysis yields the same four-domain with 14-items (3 each for growth and resourceful and 4 each for control and involvement) version as the best fit model for MeRS, with X2= 99.556, P-value=0.008, GFI=0.934, RMSEA=0.048, TLI=0953, CFI=0.965, NFI=0.899 and X2/df=1.464. Cross validation across two different hospitals for Pakistani doctors yields stable constructs of MeRS to measure resilience among medical doctors. Conclusion: This study concludes that MeRS is a valid and reliable tool for measurement of resilience in health professionals. The confirmatory factor analysis A performed on the original 37-item MeRS exhibited stable constructs. The new Modified MeRS Model with 14 items and same four domains showed high goodness of fit indices.
The relationship of fetal adrenal gland size with fetal liver length and fetal abdominal wall fat thickness in mothers with and without gestational diabetes
Authors: pouya Ensi khalili , Keshavarz Elham , moradpour moein , ghomi Zahra , Eshaghzadeh Mehdi , khazaei mehdi , mohammadi shahnaz , abolghasemzadeh farshid , zeidabadi hamideh 
Published: February 2022

Abstract
Background: Given the possible association between maternal diabetes status and metabolic disorders in the fetus, such as hepatic dysfunction or subcutaneous fat mass, it is expected that there is a close relationship between fetal adrenal gland volume and these two indicators. Aim: We decided to examine the relationship of fetal adrenal gland size with fetal liver length and fetal abdominal wall fat thickness in mothers with gestational diabetes and also to compare it with non-diabetic mothers.Methods: This cross-sectional study was performed on 40 pregnant women in the third trimester of pregnancy suffering gestational diabetes under insulin therapy as the cases and 40 age and gestational age-matched pregnant women without diabetes as the controls. An ultrasound was performed for all participants in the third trimester of pregnancy to assess adrenal gland size, liver dimensions and fetal abdominal wall fat thickness.Results: The fetuses in diabetic group was found to have adrenal glands with significantly higher mean width, but with lower height leading similarity in mean adrenal volume as compared to non-diabetic group. The mean fetal abdominal wall fat thickness was significantly higher in diabetic than in non-diabetic groups. In diabetic groups, we found a direct association between height of adrenal gland and fetal abdominal wall fat thickness (p = 0.001). The length of adrenal gland was also positively associated with abdominal wall fat thickness (p = 0.001). However in non-diabetic group, we found no association between adrenal height and fetal abdominal wall fat thickness. Measuring liver length in the two groups showed significantly higher mean value in diabetic than in non-diabetic groups. No association was revealed between adrenal volume and liver length in diabetic group. In non-diabetic group, there was a significant direct association between adrenal volume and liver length (p = 0.008). Conclusion: Gestational diabetes is directly associated with increasing fetal abdominal wall fat thickness and liver length in fetus. The dimensions of adrenal gland in fetus are also associated with fetus abdominal wall fat thickness and liver length probably independent to maternal diabetic status.
The influence of Discoidin Domain Receptor 1 expression on angiogenic factors: VEGF-A and FGF-2 in non-small cell lung cancer
Authors: Damavandi Zahra , Riahi Pardis , Majidizadeh Tayebeh , houshmand massoud 
Published: February 2022

Abstract
Purpose: Lung cancer is responsible for more cancer-related death worldwide Most lung cancer patients have non-small cell lung cancers (NSCLC), with low survival rate. Therefore, it is necessary to study new and effective targeted therapies. Discoidin domain receptor1 (DDR1) is a tyrosine kinase receptor that reacts to various collagens. Expression of DDR1 can be promoted by collagen type I, and dysregulation of DDR1 signaling are involved in several phases of tumorigenesis. Tumor growth and metastasis depend on angiogenesis caused by angiogenic factors, such as vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) secreted by tumor cells. Thus, Angiogenesis plays a critical role in cancer progression and inhibition of angiogenesis is essential for cancer treatment. The aim of the current study was to evaluate the alteration of DDR1 expression and its effects on VEGF-A, FGF-1, and FGF2 in NSCLC.Methods: A549 and Calu-3 cell lines, were treated with collagen type I and transfected with DDR1 small interfering RNA (siRNA). Then, the relative expression of DDR1, VEGF-A, FGF-1, and FGF-2 was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). The concentration of angiogenic factors was calculated by Human Angiogenesis 17-plex discovery assay. Results: According to our data, the collagen type I could stimulate DDR1 expression and DDR1 upregulation resulted in a significant increase of VEGF-A and FGF-2 expression, but did not induce FGF-1 expression. Moreover, DDR1 downregulation significantly decreased VEGF-A and FGF-2 expression. Conclusion: our findings suggest that alteration of DDR1 expression may affect VEGF-A and FGF-2 expression in NSCLC associated with tumor angiogenesis and represent a new treatment approach.
Diversity Among Synthetic Back-Derived Wheat (Triticum Aestivum L.) Lines for Drought Tolerance
Authors: Sohail Muhammad , Qamar Maqsood , Hussain Imtiaz 
Published: February 2022

Abstract
Wheat (Triticum aestivum L.) is predominantly growing in areas where terminal drought stress is the foremost yield limiting factor. Since genetic variability for climate resilience is weakened in modern wheat cultivars, exploring drought tolerance potential of the synthetic back-derived wheat lines (SBL) might help to widen genetic diversity for wheat breeders. The variation among 45 selected SBLs in response to drought stress was assessed by measuring physio-agronomic traits. The results showed a significant variation among genotypes under induced drought stress at reproductive stage in comparison to commercial wheat varieties as check. The variability among genotypes was verified by correlations, principal component analysis, heat map and clustering methods using Ward`s method. The study selected 12 synthetic back-derived wheat lines with significantly higher grain yields than 5 local checks under drought stress. The drought tolerance ability of these selected genotypes was strongly associated with proline, leaf water contents, membrane stability and better leaf chlorophyll contents under drought stress. The identified tolerant synthetic back-derived wheat lines may prove useful for wheat breeders to evolve climate resilient wheat varieties.
Development Of A Novel Light-Up Probe For Detection Of G-Quadruplexes In Stress Granules
Authors: Iida Keisuke , Sasaki Ayano , Ishida Shunsuke , Arai Takayoshi 
Published: February 2022

Abstract
G-quadruplexes (G4s) regulate various biological processes in cells. However, cellular imaging of dynamically forming G4s in biomolecular condensates using small molecules has been poorly investigated. Herein, we present a fluorescent light-up probe with the ability to selectively stabilize G4s and enhance fluorescence upon G4 binding. The foci of the probe were mainly observed in the nucleoli. These were co-localized with anti-fibrillarin antibodies and anti-G4 antibodies (BG4). Moreover, we tested detection of G4 in stress granules using the developed probe. Stress granules were induced through treatment with not only thapsigargin, but also known G4 ligands (pyridostatin, RHPS4, and BRACO-19). In the stress granules, co-localization between the probe, BG4, and stress granule markers (TIA1 and G3BP1) was detected. We present a practical light-up probe for G4s in stress granules, providing potential targets for G4 ligands.
Aucubin promoted neuron functional recovery by suppressing inflammation and neuronal apoptosis in a spinal cord injury model
Authors: Xiao Shining , Zhong Nanshan , Yang Quanming , Li Anan , Tong Weilai , Zhang Yu , Yao Geliang , Wang Shijiang , Liu Jiaming , Liu Zhili 
Published: February 2022

Abstract
Spinal cord injury (SCI) can cause severe motor impairment. Post-SCI treatment has focused primarily on secondary injury, with neuroinflammation and neuronal apoptosis as the primary therapeutic targets. Aucubin (Au), a Chinese herbal medicine, exerts anti-inflammatory and neuroprotective effects. The therapeutic effects of Au in SCI have not been reported. We showed that Au can promote functional recovery after SCI. Recovery may occur through the toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) pathway to promote M2/M1 polarization in microglia and inhibit mitochondrial dysfunction to reduce neuronal apoptosis. These biochemical changes result in reduced secondary injury and facilitate axon regeneration. Therefore, Au may be a promising post-SCI therapeutic medication.
The Primary Cilium is a Microtubule-Driven Electrical Antenna. The Case of Renal Epithelial Cells
Authors: Cantiello Horacio F , Scarinci Noelia , Gutierrez Brenda C , Cantero Maria del Rocio 
Published: February 2022

Abstract
The primary cilium is a non-motile sensory organelle that acts as a transducer of environmental cues into cellular responses. It comprises an axoneme, which is a core of microtubules (MTs), coated by a specialized membrane populated by receptors and a high density of ion channels. Dysfunctional primary cilia generate several diseases known as ciliopathies. However, the nature of ciliary signaling remains largely unknown. Herein, we determined by the patch-clamp technique, the electrical activity of cytoplasmic and axonemal MTs from LLC-PK1 renal epithelial cells. We observed electrical oscillations with fundamental frequencies at ~39 Hz and ~93 Hz in sheets of cytoplasmic MTs. We also studied isolated and in situ intact and Triton X-permeabilized primary cilia, observing electrical oscillations with peak frequencies at either 29-49 (non-permeabilized) or ~40-49 Hz (permeabilized) and ~93 Hz (both). The axonemal electrical oscillations changed with maneuvers that modify ciliary length, including addition of external Ca2+ and Li+. We also applied Continuous Wavelet Transform (CWT) and cross-correlation analyses in the Time-Frequency domain to assess the coherence between cytoplasmic and axonemal MT electrical oscillations. The evidence indicates that the primary cilium is an electrical antenna that produces MT-based electrical oscillations regulated by ciliary channels and receptors.
Feature Selection with Missing Values in DNA Microarray Gene Expression Data
Authors: Rabiei Niloofar , Soltanian Ali Reza , Farhadian Maryam , Bahreini Fatemeh 
Published: February 2022

Abstract
BackgroundImputation is one of the strategies for dealing with Missing values (MVs) in microarray data. Employing the best subset of genes for imputation is very important. In this study, we used mutual information gene selection before imputation to select the best subset of genes for imputation MVs and then classified imputed data. Two datasets were used, and we generate MVs with missing rates from 1 to 7 percent. K nearest neighbor, row mean imputation, and the method contains Feature Selection with Missing data by Mutual Information (FSM-MI) were employed. We used Root Mean Square Error (RMSE) for evaluating the performance of the methods. We classified complete and imputed data by random forest classifier and compare them by accuracy.ResultsFSM-MI imputation method with 0.0364 and 0.0083 mean RMSE value in GSE510 and GSE1063 datasets had the best performance, respectively. The classification accuracy of complete and imputed data in GSE510 and GSE1063 datasets were 100 and 80, and 100, 100, 83.3, and 66.7, and 80, 80, 70, and 70 percent in missing rates 1, 3, 5, and 7, respectively.ConclusionFeature selection before imputation MVs is as important as the selection of the best imputation method in improving the result of subsequent analyzes.
Geographic variation in top-10 prescribed medication and potentially inappropriate prescription in Portugal: An ecological study of 2.2 million older adults
Authors: Roque Fátima , Rocha Vânia , Plácido Ana , Rodrigues Daniela , Tavares Ana Barbara , Figueiras Adolfo , Herdeiro Maria Teresa 
Published: February 2022

Abstract
Background: The use of multiple medications by older adults is considered a Public Health concern since it is associated to a higher risk of adverse drug reactions and potentially inappropriate medication (PIM). This study aimed to describe the top-10 prescribed active substances in older adults considering geographical distribution and PIM prescription.Methods: A retrospective ecological study was conducted using data on the prescribed active substances during 2020 to people with 65 years or older. Information on active substances and defined daily doses (DDD) by age group, sex and region were retrieved from a Portuguese health administrative database. The average number of prescribed packages and DDD per 1000 inhabitants per day of top-10 active substances were calculated. Results: A total of 2228090 older adults (58% females) were included. The active substances with higher prescription rates (mean DDD/1000 inhabitants/day) in all ARS were furosemide and atorvastatin in both males and females, compared to the other active substances of the top-10. Geographic differences in prescription were observed (higher prescription in ARS North and Centre and lower in ARS Algarve). In females, 2/10 most prescribed active substances were PIM (benzodiazepines and opioids) with geographic disparities across regions. Conclusions: Most prescribed active substances to older adults belong to the cardiovascular system. The prescription of benzodiazepines and opioids in females, classified as PIM, alert for the need of public health policies to reduce inappropriate prescribing. Geographic differences in the top-10 most prescribed active substances and in PIM highlighted the importance of medication optimisation across regions.
Infectiousness of Places: Impact of Multiscale Human Activity Places in the Transmission of COVID-19
Authors: Liu Lun , Wang Hui , Zhang Zhu , Zhang Weiyi , Zhuang Shengsheng , Wang Shenhao , Silva Elisabete A , Lv Tingmiao , Chio Chi On , Wang Yifan , Dao Rina , Tang Chuchang , Ao-Ieong On Ieng 
Published: February 2022

Abstract
COVID-19 raises attention to epistemological risks related to everyday human activities. Our work quantifies infection transmission risks at different human activity places, including different types of settlements at macro-scale and establishments (restaurants, bars, etc.) at micro-scale, using evidences from COVID-19 in 906 urban areas across four continents. Relatively stable rules of how infection risks are distributed across human settlements and establishments are found. At micro-scale, the infection transmission risks at various establishments differ across countries, but generally, physical activity, entertainment and catering establishments lead to more infections than other activity places. At macro-scale, contrary to common beliefs, we find consistent pattern that transmission does not increase with settlement size and density. When considering interaction between the two scales, there is also consistent pattern that a smaller proportion of infections take place at specific establishments in larger settlements, suggesting that general public spaces such as streets play a greater role in transmission due to longer trips. Though with limitations, our work provides the first steps towards a system of knowledge on the linkage between places, human activities and disease transmission.
Prevalence Of Malaria; A Retrospective Study On The Trend Of Malaria From 2015 To 2021 In A Tertiary Care Centre, Puducherry
Authors: Kannambath Rachana , Rajkumari Nonika , Sivaradjy Monika 
Published: February 2022

Abstract
Battle against malaria has been going on since time immemorial. Understanding the true burden of disease and the determinants of its transmission are important for implementing adequate control measures. This study intends to explore the local epidemiology and burden of malaria in Puducherry, a coastal Union territory located in Southern part of India over a period of 7 years.A retrospective record-based study was conducted from 2015 to 2021, where details from all samples tested positive for malaria by peripheral blood examination or rapid card test, from suspected cases were collected and analyzed. The overall prevalence of malaria over the 7 years was 1.7% (257/14888). Majority of the patients were males (75.88%) and the major age group affected was from 21 to 40 years (56.03%). Disease was maximum seen during the monsoon season followed by the post monsoon season. Vivax malaria predominated irrespective of the gender, seasonal change and different age groups except in children <10 years were both falciparum and vivax malaria were seen in equivalence. Major species to cause infection among infants were Plasmodium falciparum (3/4).This study shows a declining trend of malaria transmission over the years. There is no change in the predominant species affected or seasonal trends over the years. The possibility of underestimation of cases due to various factors cannot be ignored.