bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hearing Health Perceptions and Literacy Among Primary Healthcare Providers in the United States: A National Cross-Sectional Survey
Authors: Sydlowski Sarah A. , Marinelli John P. , Lohse Christine M. , Carlson Matthew L. ,   Hearing Health Collaborative
Published: July 2022

Objective
To characterize current awareness, perceptions, and literacy surrounding hearing loss among United States primary healthcare professionals.

Study design
National cross-sectional survey study.

Setting
United States.

Participants
Four hundred six healthcare professionals.

Results
Survey respondents included 205 primary care physicians and 201 nurse practitioners or physician assistants. When compared with 10 other common health conditions, only 1% of respondents ranked hearing loss as a “most important” health condition to manage. Less than half of providers reported recommending hearing testing for their patients at least once per year, whereas evaluation of blood pressure, total cholesterol, body mass index, and blood glucose levels are recommended at least annually by more than 80% of providers. Although 95% of respondents indicated that it is somewhat important or very important for patients to know the standard definition for normal hearing, only 57% of surveyed providers know of a standard definition themselves, and only 28% reported familiarity with the concept of “20/20 hearing.” Conversely, more than 80% of respondents know the “normal” metric for blood pressure, total cholesterol, body mass index, blood glucose, and vision. Most respondents realize that hearing is important to overall health and hearing loss can impact personal safety, lead to social isolation, and negatively impact quality of life. Fifty-four percent also acknowledged a link between hearing loss and depression, but a majority were not very aware of the relationship of hearing loss to risk of falling and dementia, reduced income and job opportunities, and type 2 diabetes. Importantly, only 40% of providers believe hearing loss is treatable, and only 17% believe it is preventable.

Conclusion
Despite widespread literacy of what constitutes normal blood pressure, total cholesterol, body mass index, blood glucose, and vision metrics, healthcare providers exhibit a poor understanding of normal hearing levels. Few providers prioritize hearing health or regularly recommend for annual hearing evaluation. Most providers believe that options for people with hearing loss are limited, which may have important implications for prioritizing discussion of hearing loss with patients.
Assessing Outcomes After Adrenalectomy for Primary Aldosteronism – Early is Accurate
Authors: Suurd Diederik P.D. , Vorselaars Wessel M.C.M. , Van Beek Dirk-Jan , Borel Rinkes Inne H.M. , Spiering Wilko , Valk Gerlof D. , Vriens Menno R. 
Published: July 2022

Objective:
We aimed to investigate the postoperative trend in blood pressure (BP)-related outcomes [BP and antihypertensive (AHT) drug use] during the year following adrenalectomy for primary aldosteronism (PA) to determine the optimal timing for outcome assessment and to determine the necessary follow-up length.

Background:
Since the course of BP-related outcomes after adrenalectomy is unknown, the optimal timing of outcome assessment and follow-up duration are not clear.

Methods:
In this retrospective single center cohort study, we used a prospectively collected database with all patients referred for difficult-to-control-hypertension-analysis. All patients diagnosed with PA who underwent adrenalectomy were included. AHT drug use [in defined daily dose (DDD)] and home blood pressure measurements (HBPMs) during the first postoperative year were collected. A mixed-effects model was developed to assess the stability of DDD and HBPM over time and adjust for potential confounders.

Results:
In total 1784 patients were assessed for difficult-to-control-hypertension of whom 41 were included. Both the DDD and HBPM showed the strongest decrease in the first postoperative month (mean 1.6DDD; mean 140/85 mm Hg) compared with preoperative values (4.5DDD; 153/92 mm Hg). Thereafter, both outcomes showed a stable course from 4 to 6 months (1.6DDD; 136/86 mm Hg) up to 12 months postoperatively (2.0DDD; 136/83 mm Hg).

Conclusions:
This study showed that AHT drug use and HBPM decreased substantially within the first month after adrenalectomy for PA and afterwards generally remained stable during the year following adrenalectomy. We propose that BP-related outcomes can be assessed reliably early after adrenalectomy and question the need for routine long-term follow-up in referral centers.
In silico modelling human VPS13 proteins associated with donor and target membranes suggests lipid transfer mechanisms
Authors: Dall’Armellina Filippo , Stagi Massimiliano , Swan Laura E. 
Published: July 2022

AbstractThe VPS13 protein family constitutes a novel class of bridge-like lipid transferases. Autosomal recessive inheritance of mutations in VPS13 genes is associated with the development of neurodegenerative diseases in humans. Bioinformatic approaches previously recognised the domain architecture of these proteins. In this study, we model the first ever full-length structures of the four human homologs VPS13A, VPS13B, VPS13C, and VPS13D in association with model membranes, to investigate their lipid transfer ability and potential structural association with membrane leaflets. We analyse the evolutionary conservation and physicochemical properties of these proteins, focusing on conserved C-terminal amphipathic helices that disturb organelle surfaces and that, adjoined, resemble a traditional Venetian gondola. The gondola domains share significant structural homology with lipid droplet surface-binding proteins. We introduce in silico protein-membrane models displaying the mode of association of VPS13A, VPS13B, VPS13C, and VPS13D to donor and target membranes, and present potential models of action for protein-mediated lipid transfer.
GENOMIC INVESTIGATIONS OF ACUTE HEPATITIS OF UNKNOWN AETIOLOGY IN CHILDREN
Authors: Morfopoulou Sofia , Buddle Sarah , Torres Montaguth Oscar Enrique , Atkinson Laura , Guerra-Assunção José Afonso , Storey Nathaniel , Roy Sunando , Lennon Alexander , Lee Jack C D , Williams Rachel , Williams Charlotte A , Tutill Helena , Bayzid Nadua , Bernal Luz Marina Martin , Moore Catherine , Templeton Kate , Neill Claire , Holden Matt , Gunson Rory , Shepherd Samantha J , Shah Priyen , Cooray Samantha , Voice Marie , Steele Michael , Fink Colin , Whittaker Thomas E , Santilli Giorgia , Gissen Paul , Brown Rachel , Kaufer Benedikt B , Reich Jana , Andreani Julien , Simmonds Peter , Alrabiah Dimah K. , Hereza Sergi Castellano ,   DIAMONDS and PERFORM consortia, Andrade Catarina , Anderson Glenn , Kelgeri Chayarani , Waddington Simon N , Diaz Juan F Antinao , Hatcher James , De Surjo , Chiozzi Riccardo Zenezini , Thalassinos Konstantinos , Jacques Thomas S , Hoschler Katja , Talts Tiina , Celma Cristina , Gonzalez Suam , Gallagher Eileen , Simmons Ruth , Watson Conall , Mandal Sema , Zambon Maria , Chand Meera , Campos Luis , Martin Joanne , Thomson Emma , Ushiro-Lumb Ines , Levin Michael , Brown Julianne R , Breuer Judith 
Published: July 2022

SummarySince the first reports of hepatitis of unknown aetiology occurring in UK children, over 1000 cases have been reported worldwide, including 268 cases in the UK, with the majority younger than 6 years old. Using genomic, proteomic and immunohistochemical methods, we undertook extensive investigation of 28 cases and 136 control subjects. In five cases who underwent liver transplantation, we detected high levels of adeno-associated virus 2 (AAV2) in the explanted livers. AAV2 was also detected at high levels in blood from 10/11 non-transplanted cases. Low levels of Adenovirus (HAdV) and Human Herpesvirus 6B (HHV-6B), both of which enable AAV2 lytic replication, were also found in the five explanted livers and blood from 15/17 and 6/9 respectively, of the 23 non-transplant cases tested. In contrast, AAV2 was detected at low titre in 6/100 whole bloods from child controls from cohorts with presence or absence of hepatitis and/or adenovirus infection. Our data show an association of AAV2 at high titre in blood or liver tissue, with unexplained hepatitis in children infected in the recent HAdV-F41 outbreak. We were unable to find evidence by electron microscopy, immunohistochemistry or proteomics of HAdV or AAV2 viral particles or proteins in explanted livers, suggesting that hepatic pathology is not due to direct lytic infection by either virus. The potential that AAV2, although not previously associated with disease, may, together with HAdV-F41 and/or HHV-6, be causally implicated in the outbreak of unexplained hepatitis, requires further investigation.
Allosteric Regulation of Aptamer Affinity through Mechano-Chemical Coupling
Authors: Qu Hao , Zheng Manyi , Ma Qihui , Wang Lu , Mao Yu , Eisenstein Michael , Soh Hyongsok Tom , Zheng Lei 
Published: July 2022

AbstractThe capacity to precisely modulate aptamer affinity is important for a wide variety of applications. However, most such engineering strategies entail laborious trial-and-error testing or require prior knowledge of an aptamer’s structure and ligand-binding domain. We describe here a simple and generalizable strategy for allosteric modulation of aptamer affinity by employing a double-stranded molecular clamp that destabilizes aptamer secondary structure through mechanical tension. We demonstrate the effectiveness of the approach with a thrombin-binding aptamer and show that we can alter its affinity by as much as 65-fold. We also show that this modulation can be rendered reversible by introducing a restriction enzyme cleavage site into the molecular clamp domain and describe a design strategy for achieving even more finely-tuned affinity modulation. This strategy requires no prior knowledge of the aptamer’s structure and binding mechanism and should thus be generalizable across aptamers.
Transcriptome analysis of protein kinase MoCK2 affect acetyl-CoA metabolism and import of CK2 interacting mitochondrial proteins into mitochondria in rice blast fungus Magnaporthe oryzae
Authors: Zhang Lianhu , Shan Chonglei , Zhang Yifan , Miao Wenjing , Bing Xiaoli , Kuang Weigang , Wang Zonghua , Cui Ruqiang , Olsson Stefan 
Published: July 2022

AbstractThe rice pathogen Magnaporthe oryzae causes severe losses to rice production. Previous studies have shown protein kinase MoCK2 essential for pathogenesis, and this ubiquitous eukaryotic might affect several processes in the fungus needed for infection. To better understand which cellular processes are affected by the MoCK2 activity, we performed a detailed RNAseq analysis of deletions MoCK2-beta1 and beta2 components in relation to the background strain Ku80 and connected this analysis with the abundance of substrates for proteins in a previous pulldown of the essential CKa subunit of CK2 to estimate effects on proteins directly interacting with CK2. The results showed that MoCK2 seriously affected carbohydrate metabolism, fatty acid metabolism, amino acid metabolism and related transporters and reduced acetyl-CoA production. CK2 phosphorylation can affect the folding of proteins and especially the effective formation of protein complexes by intrinsically disordered or mitochondrial import by destabilizing soluble alpha helixes. Upregulated genes found in the pulldown of the b1 and b2 mutants indicate that proteins directly interacting with CK2 are compensatory upregulated depending on their pulldown. A similar correlation was found for mitochondrial proteins. Taken together, the classes of proteins and the change in regulation in the b1 and b2 mutants suggest that CK2 has a central role in mitochondrial metabolism, secondary metabolism, and ROS resistance, in addition to the previously suggested role in the formation of new ribosomes, all processes central to efficient non-self responses as innate immunity.ImportanceThe protein kinase CK2 is highly expressed and essential for plants, animals, and fungi affecting fatty acid-related metabolism. In addition, it directly affects the import of essential mitochondrial proteins into mitochondria. These effects mean CK2 is essential for lipid metabolism and mitochondrial function and, as shown before, crucial for making new translation machinery proteins. Taken together, our new results combined with previous published indicate that CK2 is an essential protein necessary for the capacity to launch efficient innate immunity responses and withstand the negative effect of such responses necessary for general resistance against invading bacteria and viruses as well as to interact with plants and withstand plant immunity responses and kill plant cells.Protein kinase CK2, highly expressed and essential for plants, animals, and fungi, affects fatty acid-related metabolism and mitochondrial proteins, making it essential for the capacity to launch efficient innate immunity responses and plant pathogenicity
Is conflict useful after all? A scenario experiment on the antecedents and consequences of task and relationship conflict in military settings
Authors: Telecan Maria Ioana , Rus Claudia Lenuța , Curșeu Petru Lucian 
Published: July 2022

Purpose
Using a within-subject vignette experiment, this study aims to disentangle the differential effect of task and relationship conflict on team effectiveness and interpersonal outcomes. In addition, the authors aimed to test the moderating role of the conflict inducing person’s gender on task and relationship conflict outcomes.

Design/methodology/approach
Data were collected using four vignettes administered to 151 participants from an Air Force military organization.

Findings
Relationship conflict was more damaging than task conflict for team effectiveness, the harmonious interpersonal relations and the expected positive affective team climate. Task conflict triggers more positive emotions than relationship conflict and, in consequence, team effectiveness is higher in scenarios with the task rather than relationship conflict. The groups in which conflict is induced by a woman are not expected to perform better than groups in which the conflict is induced by a man. The similarity between participant’s gender and the gender of the person that generated the conflict accentuates rather than attenuates the effectiveness decline associated with relationship conflict. Task conflict triggers cooperative, while relationship conflict triggers assertive conflict management strategies.

Research limitations/implications
This study provided only a snapshot on how task and relationship conflict are perceived to relate to the various team and individual-level outcomes, particularly in military settings.

Originality/value
The findings evidence the differential effect of task and relationship conflict not only on team effectiveness but also on attitudinal and emotional team and individual-level outcomes.
All that glistens is not (green) gold: historicising the contemporary chlorophyll fad through a multimodal analysis of Swedish marketing, 1950–1953
Authors: O'Hagan Lauren Alex 
Published: July 2022

Purpose
This paper aims to historicise the contemporary chlorophyll trend through the first academic study of its early marketing in Sweden (1950–1953). Using multimodal critical discourse analysis, it demonstrates how brands used advertisements to convince female consumers of chlorophyll’s necessity to fulfil certain aspirational goals.

Design/methodology/approach
In all, 150 advertisements for chlorophyll products were collected from the Swedish Historical Newspaper Archive, as well as 600 additional advertisements for the three most popular products (toothpaste/mouthwash, sanitary towels and soap) from 1940 to 1950 and from 1954 to 1964. Then, multimodal critical discourse analysis was used to investigate how the products were marketed before, during and after the chlorophyll trend, identifying the general themes and linguistic/semiotic structures of the advertisements.

Findings
This paper shows how the commercial use of chlorophyll offered a lucrative opportunity for marketers, acting as a “tabula rasa” on which they could use discourses of science, nature, idealised femininity and luxury to draw connections with health, modernity and beauty, despite the product having no real purpose or value.

Originality/value
Viewing this fad from a historical perspective emphasises how brands, marketers and influencers continue to capitalise on the anxieties of female consumers with promises around beauty, hygiene and health. It, thus, offers us critical distance to reflect on contemporary claims about chlorophyll’s health benefits to make informed choices.
Understanding the key drivers in using mobile payment among Generation Z
Authors: Lisana Lisana 
Published: July 2022

Purpose
This quantitative study aims to examine the determinants that impact the behavioral intention to use mobile payment (m-payment) among Generation Z (Gen Z) customers in Indonesia.

Design/methodology/approach
The theoretical model comprises seven latent variables: effort expectancy, performance expectancy, social influence, facilitating conditions, promotional activities, perceived security and behavioral intention. In addition, the two moderating factors of education and gender are used to investigate the significant effect of the determinants on intention to adopt m-payment. This study obtained the final data size of 430 respondents. The data analysis is conducted using structural equation modeling.

Findings
The results substantiate the significance of promotional activities, perceived security, performance expectancy, effort expectancy and social influence, on the behavioral intention to accept m-payment systems. Gender is revealed to significantly moderate two constructs: social influence and promotional activities, on the m-payment usage intention. Meanwhile, education moderates the effect of perceived security on behavioral intention.

Originality/value
This research is expected to fill the gap because only a few studies discuss the determinants affecting m-payment usage in Indonesia, especially among Gen Z-ers. Furthermore, the new findings associated with the role of two moderating factors become important practical implications because most of the prior studies often ignore the moderating factors.
Cotton fibre selection based on quality value using measurement of alternatives and ranking according to compromise solution (MARCOS) method
Authors: Mitra Ashis 
Published: July 2022

Purpose
Cotton fibre lots are graded and selected for yarn spinning based on their quality value which is a function of certain fibre properties. Cotton grading and selection has created a domain of emerging interest among the researchers. Several researchers have addressed the said issue using a few exponents of multi-criteria decision-making (MCDM) technique. The purpose of this study is to demonstrate a cotton selection problem using a recently developed measurement of alternatives and ranking according to compromise solution (MARCOS) method which can handle almost any decision problem involving a finite number of alternatives and multiple conflicting decision criteria.

Design/methodology/approach
The MARCOS method of the MCDM technique was deployed in this study to rank 17 cotton fibre lots based on their quality values. Six apposite fibre properties, namely, fibre bundle strength, elongation, fineness, upper half mean length, uniformity index and short fibre content are considered as the six decision criteria assigning weights previously determined by an earlier researcher using analytic hierarchy process.

Findings
Among the 17 alternatives, C9 secured rank 1 (the best lot) with the highest utility function (0.704) and C7 occupied rank 17 (the worst lot) with the lowest utility function (0.596). Ranking given by MARCOS method showed high degree of congruence with the earlier approaches, as evidenced by high rank correlation coefficients (Rs > 0.814). During sensitivity analyses, no occurrence of rank reversal is observed. The correlations between the quality value-based ranking and the yarn tenacity-based rankings are better than many of the traditional methods. The results can be improved further by adopting other efficient method of weighting the criteria.

Practical implications
The properties of raw cotton have significant impact on the quality of final yarn. Compared to the traditional methods, MCDM is reported as the most viable solution in which fibre parameters are given their due importance while formulating a single index known as quality value. The present study demonstrates the application of a recently developed exponent of MCDM in the name of MARCOS for the first time to address a cotton fibre selection problem for textile spinning mills. The same approach can also be extended to solve other decision problems of the textile industry, in general.

Originality/value
Novelty of the present study lies in the fact that the MARCOS is a very recently developed MCDM method, and this is a maiden application of the MARCOS method in the domain of textile, in general, and cotton industry, in particular. The approach is very simple, highly effective and quite flexible in terms of number of alternatives and decision criteria, although highly robust and stable.
Commentary on “Development of LDPAQ: learning disability physical activity questionnaire”
Authors: Brooker Katie , Hill Jessica 
Published: July 2022

Purpose
This paper aims to discuss the importance of delivering high-quality education to allied health and fitness professionals around physical activity for people with intellectual disabilities.

Design/methodology/approach
The clinical training and experience needs of allied health and fitness professionals are examined in relation to knowledge and skills around physical activity levels and people with intellectual disabilities.

Findings
The training of allied health and fitness professionals may not be adequate. Current training does not appear to provide professionals with the skill set and suitable experiences to appropriately support people with intellectual disabilities to be active.

Originality/value
Key training areas for allied health and fitness professionals are outlined.
Customer responses to airline companies' service failure and recovery strategies: the moderating role of service failure habit
Authors: Elbaz Ahmed Mohamed , Soliman Mohammad , Al-Alawi Alamir , Al-Romeedy Bassam Samir , Mekawy Moustafa 
Published: July 2022

Purpose
The purpose of this study is to investigate the perceived severity of service failure (PSSF) and service recovery strategies (SRSs) as antecedents of customer forgiveness (CF), customer reconciliation, negative word-of-mouth and re-patronage intention as outcomes across two Middle Eastern countries: Egypt and Oman.

Design/methodology/approach
A quantitative technique was used, using a questionnaire, to collect data from customers of the national airline companies of Egypt and Oman. The perspectives of 1,084 airline customers were analysed using partial least squares-structural equation modelling.

Findings
The results of this study show that service failure severity has a negative influence on the effectiveness of SRSs. In turn, proper SRSs strongly and positively affect both Egyptian and Omani CF. Furthermore, the findings of this study indicate that service failure habit plays an important role in increasing the negative influence of perceived service failure severity on Egyptian, but not Omani, CF. Overall, results suggest that CF differs considerably between Egyptians and Omanis.

Originality/value
This study developed and empirically examined a comprehensive conceptual model of the drivers and outcomes of CF of airline companies. This study provides academics with meaningful insights into how social and cultural differences between customers in different countries can result in sometimes dramatically different behaviours following a service failure.