bluuebunny/crossref_metadata_2025_split huggingface dataset:
Access to Health and Mortality Rate for Pancreatic Cancer.
Authors: Bernardes Ellen , Sousa Luiz , Adami Fernando , Paiva Laercio da Silva , Abreu Luiz 
Published: June 2022

Abstract
OBJECTIVE: Among all types of cancers diagnosed in Brazil, pancreatic cancer corresponds to 2%, about 1 in 65 individuals. To evaluate rates of hospital admissions and mortality from pancreatic cancer in Brazilian regions. RESULTS: In the analysis of the Brazilian regions, there were 64,587 hospitalizations between men and women aged 54, over 80 years old. The incidence of pancreatic cancer increased, resulting in deaths and hospitalizations in the Southeast and South regions, respectively. In the analysis of the Brazilian regions, there were 64,587 hospitalizations between men and women, aged 54 years to over 80 years. The incidence of pancreatic cancer showed an increase in deaths and hospitalizations in the Southeast and South regions, respectively. The age range of death between men and women was the same. In men over 80 years old, within a percentage of 17.02% and in women aged 23.19%. In the Northeast region, all age groups among men showed significance above 80 years (β = 3.01; p <0.000;) and also among women over 80 years (β = 3.73; p <0.000;).
The growth and lipid accumulation of Scenedesmus quadricauda under nitrogen starvation stress during xylose mixotrophic/heterotrophic cultivation
Authors: Mou Yiwen , Liu Na , Su Kunyang , Li Xue , Lu Tianxiang , Yu Ze , Song Mingming 
Published: June 2022

Abstract
In order to conquer the block of high cost and low yields which limit to realize the commercialization of microalgal biodiesel, the mixotrophic and heterotrophic cultivation of Scenedesmus quadricauda FACHB-1297 fed on xylose was studied separately employing six forms of media: phosphorus sufficient, phosphorus restricted and phosphorus starvation were combined with nitrogen sufficient and nitrogen starvation conditions. The maximum oil percentage (about 41% of dry weight) was obtained on the 5th day (heterotrophic cultivation) and 8th day (mixotrophic cultivation) under the nitrogen starved and phosphorus sufficient (N0&P) conditions, which was about 2-fold in comparison to the initial lipid content on the sufficient nitrogen condition (control). Under mixotrophic and heterotrophic modes, the highest lipid productions were both achieved in the N0&P trial, with the value of 274.97 mg/L and 193.77 mg/L, successively. Xylose utilization rate of 30%-96% under heterotrophic modes was apparently higher than that of 20%-50% in mixotrophic modes. In contrast, phosphorus uptake rate of 100% under mixotrophic modes was significantly more than that of 60%-90% in heterotrophic cultivation. Furthermore, under xylose heterotrophic cultivation, the phosphorus had a positive impact on microalgae cell synthesis, and the oil content enhanced with the augmentation in phosphorus concentrations. We suggest that sufficient phosphorus should be supplied for obtaining more microalgal lipid production in the lack of nitrogen for xylose heterotrophic and mixotrophic conditions. This is a highly effective way to obtain efficient microalgae biofats production.
Prevenance of SCC mec type V, Novel ST 243 Methicillin-Resistant Staphylococcus aureus strain from Indian seafood products
Authors: Sivaraman Gopalan Krishnan , Gupta Sobin , Sivam Visnuvinayagam , T Muthulakshmi , Elanovan Ravikrishnan , Perumal Vivekanandan , Balasubramanium Ganesh , Lodha Tushar , Yadav Amit 
Published: June 2022

Abstract
Compared to the clinical sector, the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in the food sector is relatively low. However, their presence in seafood is a significant public health concern. In India, fish and fishery products are maximally manually handled compared to other food products. In this study, 498 fish samples were collected under various conditions (fresh, chilled or dressed) and representatives from their surroundings. These samples were screened for the incidence of coagulase-positive Staphylococcus aureus (CPS), determining its antimicrobial resistance, MRSA and genetic profile. It is observed that 15.0% and 3.0% of the total samples were screened positive for CPS and MRSA. The S. aureus strain MRSARF-10 showed higher resistance to linezolid, co-trimoxazole, cefoxitin, ofloxacin, gentamicin, rifampicin, ampicillin/sulbactam and Piperacillin–tazobactam. This MRSA, spa type t021 and SCC mec type V strain isolated from dried ribbon fish (Family Trachipteridae) carried virulence factors for exoenzymes such as aureolysin, serine, toxin genes and a novel MLST ST 243, as revealed from its draft-genome sequence. This highly pathogenic, multidrug-resistant and virulent S. aureus novel strain is circulating in the environment with chances of spreading among the seafood workers and the environment. It is further suggested that Good Hygienic Practices recommended by World Health Organization need to be followed during the different stages of seafood processing to provide pathogen-free fish and fishery products to the consumers.
Flexoelectric polarizing and control of a ferromagnetic metal
Authors: Peng Wei , Park Se Young , Roh Chang Jae , Mun Junsik , Ju Hwiin , Kim Jinkwon , Ko Eun Kyo , Liang Zhengguo , Hahn Sungsoo , Zhang Jinfeng , Si Liang , Jo Yong Jin , Jo Yongjoo , Kim Tae Heon , Kim Changyoung , Wang Lingfei , Kim Miyoung , Lee Jong Seok , Noh Tae Won , lee Daesu 
Published: June 2022

Abstract
Electric polarization can only be well defined in insulators, not in metals, and there is no general scheme to induce and control bulk polarity in metals. Here, we circumvent this limitation by utilizing a pseudo-electric field generated by inhomogeneous lattice strain, namely a flexoelectric field, as a universal means of polarizing and controlling a metal. Using heteroepitaxy and atomic-scale imaging, we show that flexoelectric fields polarize the bulk of an otherwise centrosymmetric metal SrRuO3, with remarkable off-center displacements of Ru ions. This further impacts the electronic bands and lattice anisotropy of the flexo-polar SrRuO3, potentially leading to an enhancement of electron correlation, ferromagnetism, and its anisotropy. Beyond conventional electric fields, flexoelectric fields may universally engender novel electronic states and their control via pure atomic displacements.
Exploring Optimal Reaction Conditions Guided by Graph Neural Networks and Bayesian Optimization
Authors: Choi Youn-Suk , Kwon Youngchun , Lee Dongseon , Kim Jin Woo , Kim Sun 
Published: June 2022

Abstract
The optimization of organic reaction conditions to obtain the target product in high yield is crucial to avoid expensive and time-consuming chemical experiments. Advancements in artificial intelligence have enabled various data-driven approaches to predict suitable chemical reaction conditions. However, for many novel syntheses, experimental optimization of the reaction conditions is inevitable. Bayesian optimization (BO), an iterative global optimization algorithm, demonstrated exceptional performance to identify suitable reagents compared to synthesis experts. However, BO requires several initial randomly selected experimental results (yields) to train yield prediction surrogate models (approximately 10 experimental trials). In addition, parts of this process, such as the cold-start problem in recommender systems, are inefficient. Here, we present an efficient BO algorithm for reaction optimization with a message passing neural network trained on vast amounts of experimental data. We demonstrated the effectiveness of the proposed algorithm on benchmarks and in-house datasets with reference to optimization methods and human experts.
Possibilities of Using the Duplex System Plasma Nitriding + CrN Coating for Special Components
Authors: Dobrocky David , Pokorny Zdenek , Joska Zdenek , Vitek Roman , Prochazka Jiri , Studeny Zbynek , Sedlak Josef , Slany Martin 
Published: June 2022

Abstract
The purpose of material selection and surface treatment for a given component is to assure the weapon's failure-free and dependable operation throughout its life time. At the same time, the material and surface technology chosen must take into account the chosen manufacturing method in terms of material attributes, as well as the economics and ecology of production. A gas piston is one of the most visible parts of high-performance small weapons (automatic rifles) that provides its own drive (automation of the functional cycle). The pressure and temperature of the propellant gases, as well as dynamic shocks and wear, put a lot of strain on the gas piston, which puts a lot of strain on the piston material. Steel C55, 80Mn4, 31NiCr14, 42CrMo4, X30Cr13, and X30WCrV9.3 are the most common materials for gas pistons, which are hard chrome plated. Hard chromium plating, which is extremely damaging to the environment and human health, is currently being replaced by PVD or CVD methods. PVD coating deposition processes, for example, have shown to be viable options. Surface treatments including the application of layers and subsequent deposition of coatings are one option (duplex system). The article discusses the use of CrN coating to replace hard chromium plating and the duplex system plasma nitriding + CrN coating (hence PN + CrN). For testing, 42CrMo4 steel was chosen. The strong chrome coating as well as the CrN coating itself were compared to the PN + CrN duplex system. The mechanical and tribological properties, as well as the shape and texture of the surface, were all examined. For exposed sections of small weapons, the PN + CrN duplex system has proven to be a viable alternative to hard chrome coating.
TMSG1/LASS2 inhibits cell proliferation, invasive ability through a novel transcriptional regulation way mediated by Homeodomain Running title: a novel transcription regulation role of TMSG1
Authors: Wang Xin , Xia Yan , Li Jingjing , Xiang Fenggang , Yu Wenjuan 
Published: June 2022

Abstract
Background: TMSG1/LASS2 is emerging as an effective tumor suppressor as a transmembrane protein since it has been first cloned. We for the first time found by accident in our previous study TMSG1/LASS2 could be located in the nucleus, and it contained Homeodomain and nuclear localization signal (NLS). We aimed to investigate whether TMSG1/LASS2 possessed the function of transcription factor and identify the target genes regulated by the transcriptional regulation function of TMSG1/LASS2.Methods: We constructed and transfected three variants containing different functional domains of TMSG1/LASS2 to human prostate cancer cell PC-3M-1E8. Immunofluorescence was performed to detect the cellular localization of the three variants and identified its functional domain mediating it entering the nucleus. Chromatin immunoprecipitation (ChIP) associated with high-throughput sequencing was performed to select the target genes binding to TMSG1/LASS2 protein. Then we investigated the transcriptional regulation effect of TMSG1/LASS2 on one of the target genes Neural Proliferation, Differentiation and Control 1 (NPDC1) by dual-luciferase reporter system, and examined the expression of NPDC1 upon the overexpression or knockdown of TMSG1/LASS2, as well as the cell proliferation ability, apoptosis and cell cycle. Results: Immunofluorescence result revealed the variants deleted Homeodomain or NLS could not enter the nucleus. Neural Proliferation, Differentiation and Control 1 (NPDC1) gene, whose promoter fragment was obtained from ChIP using TMSG1/LASS2 antibody for further study. Dual-Luciferase reporter system revealed TMSG1/LASS2, as well as the variant with the deletion of NLS could enhance the transcription of NPDC1 promoter rather than the variant deleted Homeodomain. RT-qPCR and Western blot results demonstrated the gene and protein levels of NPDC1 were significantly increased upon TMSG1/LASS2 overexpression other than the variants without Homeodomain or NLS. Moreover, the level of NPDC1 were markedly decreased upon TMSG/LASS2 knockdown. Furthermore, the full length TMSG1/LASS2 was more able to inhibit cell proliferation, invasive ability and promote the cell apoptosis than the variants without Homeodomain or NLS. Conclusions: TMSG1/LASS2 could enter the nucleus to play transcription factor role mediated by its Homeodomain and NLS. TMSG1/LASS2 could promote the transcription and subsequent expression of NPDC1, one of its target genes. TMSG-1/LASS2 might inhibit cell proliferation, invasive ability, promote cell apoptosis and G0/G1 cell cycle arrest partially mediated by its Homeodomain and NLS. We for the first time propose a new transcriptional regulation function of TMSG1/LASS2 in addition to the known tumor suppressor function as a transmembrane protein.
In vivo and in vitro validation of GATA-3 suppression for correcting impaired adipogenesis, restoring insulin sensitivity, and lowering risk of T2D
Authors: Al-Jaber Hend , Mohamed Nura A , Govindharajan Vijay K , Taha Samir , John Jomon , Halim Sharique , Alser Maha , Al-Muraikhy Shamma , Anwardeen Najeha , Agouni Abdelali , Elhissi Abdelbary , Al-Naemi Hamda A. , Al-Mansoori Layla , Elrayess Mohamed A 
Published: June 2022

Abstract
Purpose
Impaired adipogenesis is associated with development of insulin resistance and increased risk of type 2 diabetes (T2D). GATA Binding Protein 3 (GATA3) is implicated in impaired adipogenesis and the onset of insulin resistance. Therefore, we hypothesize that inhibition of GATA3 could promote adipogenesis, restore healthy fat distribution, and enhance insulin signaling.
Methods
BALB/c mice were treated with GATA3 inhibitor (DNAzyme hgd40) over a period of two weeks. Liposomes loaded with DNAzyme, pioglitazone, or vehicle controls were administered subcutaneously every two days, at the right thigh. At the end of the study, adipose tissues were collected and weighed from the site of injection, the opposite side, and the omental tissues. Anti-oxidant enzyme (SOD and catalase) activities were assessed in animals’ sera, and gene expression was measured using well-established protocols. Primary human preadipocytes were treated with DNAzyme hgd40. Cell proliferation, adipogenic capacity, gene expression, and insulin signaling were measured following well-established protocols.
Results
In vivo GATA3 inhibition promoted adipogenesis at the site of injection and reduced MCP-1 expression. GATA3 inhibition also reduced omental tissue size and PPARγ expression. Additionally, in vitro GATA3 inhibition induced adipogenesis of primary human preadipocytes, and enhanced insulin signaling through reduced expression of p70S6K.
Conclusions
These findings suggest that modulating GATA3 expression offers a potential therapeutic benefit by correcting impaired adipogenesis, promoting healthy fat distribution, restoring insulin sensitivity and lowering the risk of T2D.
Advanced tailings dam performance monitoring with seismic noise and stress models
Authors: Ouellet Susanne , Dettmer Jan , Olivier Gerrit , de Wit Tjaart , Lato Matthew 
Published: June 2022

Abstract
Tailings dams retain the waste by-products of mining operations and are amongst the world’s largest engineered structures. Recent tailings dam failures highlight important gaps in current monitoring methods and a pressing need to advance research on tailings dam monitoring technologies, considering growth predictions for the mining of metals. At an active tailings dam in northern Canada, we combine ambient noise interferometry with a quantitative stress model to monitor shear wave velocity (Vs) changes. Changes in seismic velocities of less than 1% correlate strongly with water level fluctuations at the adjacent tailings pond. A stress model, calibrated using pond level recordings and Vs profiles obtained from cone penetration tests, demonstrates that the seismic velocity changes obtained with ambient noise interferometry are predominantly changes in Vs. Furthermore, this model constrains Vs changes to a depth of ~16 m, corresponding to uncompacted tailings below the dam. As Vs is used to assess the liquefaction potential of soils, this method provides important advances for understanding changes in dam performance over time.
Associations between serum mitokine levels and outcomes in stable COPD
Authors: Amado Carlos A. , Martín-Audera Paula , Agüero Juan , Lavín Bernardo A , Guerra Raúl A , Muñoz Pedro , Berja Ana , Casanova Ciro , García-Unzueta Mayte 
Published: June 2022

Abstract
Background: Mitokines (Humanin (HN), GDF15 and FGF21) are produced as a result of mitochondrial dysfunction and may have major roles in chronic inflammation, malnutrition and exercise capacity in people with COPD. Methods: A total of 165 patients with stable COPD and 49 smokers without COPD were enrolled. We assessed their serum mitokine levels and clinical characteristics at baseline. We recorded moderate and severe exacerbation for the next 12 months.Results: Baseline serum HN (p=0.037) and GDF15 (p= 0.013) levels were higher in the COPD group. High HN levels were associated with a high risk of exacerbation (HRE) (p=0.011), malnutrition (p=0.004) 6MWD (p=0.008), and future moderate (p=0.007) and severe exacerbations (p=0.009). High GDF15 levels were associated with HRE (p=0.027), 6MWD (p=0.017) and desaturation in 6MWT (p=0.006). High FGF21 levels were predicted future severe exacerbation (p=0.008).Conclusions: The mitokine levels were higher in patients with COPD than controls, and were associated with important clinical outcomes. Among the mitokines, HN showed the strongest relationship with COPD and may serve as a future risk biomarker in this disease.Trial registry: ClinicalTrials.gov; No.: NCT04449419; URL: www.clinicaltrials.gov. Date of registration: June 26, 2020.
Hankel Norm based Strict Passivity Performance of Digital Filter using Saturation Nonlinearities
Authors: S Ronica B I , P Grace Kanmani 
Published: June 2022

Abstract
The work proposes a new technique to examine and reduce the unusual memory effects in digital filters with saturation nonlinearity when external interference is present via strict passivity approach. Novel criterion is given to show the performance of Hankel norm in interfered digital filters by using strict passivity approach. The unusual behaviour of digital filters about previous excitations can be checked by using this proposed criterion. Given criterion ensures the asymptotic stability nature when there is no external input. The dominance of the proposed method is represented by a mathematical example.
Study on the critical groundwater depth of controlling risk management in a semi-arid irrigated area in China based on recharge-discharge relationship
Authors: jing haihua , Zhang Jing , Dong Kebao B. , Jin Zexu , Ma Jiaqi , Yan Yuru 
Published: June 2022

Abstract
The imbalance between exploitation and recharge and the yearly decline in water tables is a common water resource problem in semi-arid areas in China. To ensure the sustainable use of groundwater and the stability of the regional hydrological cycle, a reasonable groundwater control table need to be determined to achieve a balance between groundwater exploitation and recharge. Based on the characteristics of the vertical hydrological cycle in semi-arid irrigation areas, this paper used a combination of in situ field experiments and software simulations to calculate the groundwater infiltration recharge under three types of subsurface conditions, namely, drip irrigation under mulch, border irrigation, and bare area, and to analyze the relationship between groundwater infiltration recharge and groundwater table. Based on the relationship between groundwater recharge and discharge, the critical groundwater depth of maintaining exploitation and recharge balance (CGDM) for drip irrigation under mulch was calculated to be 131.52-187.15 cm, and the critical groundwater depth of disrupting exploitation and recharge balance (CGDD) was 307.66-363.67 cm. For border irrigation, the CGDM was 80.00-84.34 cm and the CGDD was 198.87-248.44 cm. It also proposed a groundwater table management strategy to address the risk of imbalance in regional groundwater exploitation and recharge. The critical groundwater depth of controlling risk management (CGDC) can be used for regional groundwater management and guidance for agricultural irrigation, providing technical support for achieving regional groundwater exploitation and recharge balance and maintaining a stable hydrological cycle.
PVA/CMC/Attapulgite clay composite hydrogel membranes for biomedical applications: Factors affecting membranes crosslinking and bio-evaluation tests
Authors: Farid Eman , Kamoun Elbadawy A , Taha Tarek H. , El-Dissouky Ali , Khalil Tarek E. 
Published: June 2022

Abstract
Herein, polyvinyl alcohol-carboxymethyl cellulose (PVA-CMC) composite hydrogel membranes were prepared using solution-casting method, where citric acid (CA) was added as crosslinker in different ratios of (7, 10 and12 wt.%). Attapulgite clay extracted from Northwestern Desert of Borg El-Arab, Egypt; was incorporated as nanofiller (1, 2, 4, and 5 wt.%) into membranes for improving their mechanical/ thermal stability. Results revealed that, physicochemical properties of membranes e.g. swelling%, tensile strength and morphology of membranes affected significantly by different clay concentrations and citric acid crosslinker. Also, attapulgite clay with concentration 1 (wt.%) enhanced mechanical strength of composite membranes, compared to other clay concentrations. Furthermore, protein adsorption %, hydrolytic degradation, hemolysis (%) and antimicrobial activity significantly affected by clay contents and CA concentrations. Four bacterial pathogens e.g. Candida albicans, Escherichia coli, Klebsiella pneumoniae, and Bacillus cereus were used for testing antimicrobial activity of prepared membranes. Results referred to increasing of clay contents led to a high hemolysis %; however, increasing CA concentration significantly reduced hemolysis %. Meanwhile, membranes with low clay contents offered the most effective resistance against tested microbes. These findings are referring to the ability of using PVA-CMC-attapulgite composite membranes crosslinked by CA as good candidate of biomaterials for dermal wound dressings.
Water Soluble and Insoluble Components of PM2.5 in Nanchang City and Their Cytotoxic Research on Epithelial Cells (A549)
Authors: Liu Xiaozhen , Wang Lan , Ren Yufeng 
Published: June 2022

Abstract
To investigate the toxic effects of PM2.5 on human lung epithelial cells A549 and the potential mechanism of action, this research intends to prepare water-soluble (WS-PM2.5) and insoluble components (WIS-PM2.5) of PM2.5 by collecting atmospheric fine particles. The samples of atmospheric PM2.5 at the Qianhu Campus of Nanchang University in Nanchang city were collected on this subject. The desiccated PM2.5 was prepared into water-soluble and insoluble components, The cell toxicity of WS-PM2.5 and WIS-PM2.5 was assessed by the MTT method measuring the cell survival rate. According to the results of the MTT test, the exposure time was selected 24 h for all subsequent tests. The lactate dehydrogenase (LDH) and intracellular superoxide dismutase (SOD) in the cell supernatant fluid were measured by the trace enzyme method. Cell reactive oxygen species (ROS) were measured by flow cytometry, and the expression of cell inflammatory factors IL-6 and TNF-α were measured by ELISA and RT-QPCR. The results showed that WS-PM2.5 and WIS-PM2.5 can inhibit the activity of A549 cells, destroy the integrity of cell membranes, promote oxidative stress in cells, and induce inflammatory reactions in cells, thereby producing toxic effects on A549 cells. However, their toxic mechanism is different, and the toxicity of the cells is significantly different. The toxic effect of WIS-PM2.5 is significantly stronger than that of WS-PM2.5 in inhibiting cell viability, cell membrane damage, and oxidative stress, while significantly lower than that of WS-PM2.5 in causing inflammatory damage.