bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dysfunction of parvalbumin-expressing cells in the thalamic reticular nucleus induces cortical spike-and-wave discharges and an unconscious state
Authors: Abdelaal Manal S. , Midorikawa Mitsuharu , Suzuki Toru , Kobayashi Kenta , Takata Norio , Miyata Mariko , Mimura Masaru , Tanaka Kenji F. 
Published: January 2022

Abstract
Spike-and-wave discharges and an accompanying loss of consciousness are hallmarks of absence seizure, which is a childhood generalized epilepsy disorder. In absence seizure, dysfunction of the cortico-thalamo-cortico circuitry is thought to engage in abnormal cortical rhythms. Previous studies demonstrated that the thalamic reticular nucleus has a critical role in the formation of normal cortical rhythms; however, whether thalamic reticular nucleus dysfunction leads directly to abnormal rhythms, such as epilepsy, is largely unknown. We found that expressing the inhibitory opsin, archaerhodopsin, including in the thalamic reticular nucleus, caused abnormal cortical rhythms in Pvalb-tetracycline transactivator::tetO-ArchT (PV-ArchT) double transgenic mice. We validated the PV-ArchT line as a new mouse model of absence seizure through physiological and pharmacological analyses, as well as through examining their behavioural features. We then discovered that archaerhodopsin expression exclusively in thalamic reticular nucleus parvalbumin-positive neurons was sufficient to induce cortical spike-and-wave discharges using adeno-associated virus-mediated thalamic reticular nucleus targeting. Furthermore, we found that archaerhodopsin expression impaired rebound burst firing and T-current in thalamic reticular nucleus parvalbumin-positive cells by slice physiology. Although T-current in the thalamic reticular nucleus was impaired, the T-current blocker ethosuximide still had a therapeutic effect in PV-ArchT mice, suggesting a gain of function of T-type calcium channels in this absence seizure model. However, we did not find any over- or misexpression of T-type calcium channel genes in the thalamus or the cortex. Thus, we demonstrated that thalamic reticular nucleus dysfunction led to an absence seizure-like phenotype in mice. In a final set of experiments, we showed that the archaerhodopsin-mediated absence seizure-like phenotype disappeared after the removal of archaerhodopsin by using a time-controllable transgenic system. These data may provide a hint as to why many absence seizures naturally regress.
Spatiotemporal target selection for intracranial neural decoding of abstract and concrete semantics
Authors: Nagata Keisuke , Kunii Naoto , Shimada Seijiro , Fujitani Shigeta , Takasago Megumi , Saito Nobuhito 
Published: January 2022

Abstract
Decoding the inner representation of a word meaning from human cortical activity is a substantial challenge in the development of speech brain–machine interfaces (BMIs). The semantic aspect of speech is a novel target of speech decoding that may enable versatile communication platforms for individuals with impaired speech ability; however, there is a paucity of electrocorticography studies in this field. We decoded the semantic representation of a word from single-trial cortical activity during an imageability-based property identification task that required participants to discriminate between the abstract and concrete words. Using high gamma activity in the language-dominant hemisphere, a support vector machine classifier could discriminate the 2-word categories with significantly high accuracy (73.1 ± 7.5%). Activities in specific time components from two brain regions were identified as significant predictors of abstract and concrete dichotomy. Classification using these feature components revealed that comparable prediction accuracy could be obtained based on a spatiotemporally targeted decoding approach. Our study demonstrated that mental representations of abstract and concrete word processing could be decoded from cortical high gamma activities, and the coverage of implanted electrodes and time window of analysis could be successfully minimized. Our findings lay the foundation for the future development of semantic-based speech BMIs.
Variational dimensions of cingulate cortex functional connectivity and implications in neuropsychiatric disorders
Authors: Lam Yin-Shing , Li Jiaxin , Ke Ya , Yung Wing-Ho 
Published: January 2022

Abstract
Significant variations in brain functional connectivity exist in the healthy population, rendering the identification and characterization of their abnormalities in neuropsychiatric disorders difficult. Here, we proposed a new principal component analysis (PCA) approach to study variations in functional connectivity, focusing on major hubs of the salience network and default mode network, namely the anterior and posterior cingulate cortices. We analyzed the intersubject variability of human functional magnetic resonance imaging connectivity obtained from healthy, autistic, and schizophrenic subjects. Utilizing data from 1000 Functional Connectomes Project, COBRE, and ABIDE 1 database, we characterized the normal variations of the cingulate cortices with respect to top PCA dimensions. We showed that functional connectivity variations of the 2 cingulate cortices are constrained, in a parallel manner, by competing or cooperating interactions with different sensorimotor, associative, and limbic networks. In schizophrenic and autistic subjects, diffuse and subtle network changes along the same dimensions were found, which suggest significant behavioral implications of the variational dimensions. Furthermore, we showed that individual dynamic functional connectivity tends to fluctuate along the principal components of connectivity variations across individuals. Our results demonstrate the strength of this new approach in addressing the intrinsic variations of network connectivity in human brain and identifying their subtle changes in neuropsychiatric disorders.
Symptoms Compatible With Long Coronavirus Disease (COVID) in Healthcare Workers With and Without Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Infection—Results of a Prospective Multicenter Cohort
Authors: Strahm Carol , Seneghini Marco , Güsewell Sabine , Egger Thomas , Leal-Neto Onicio , Brucher Angela , Lemmenmeier Eva , Meier Kleeb Dorette , Möller J Carsten , Rieder Philip , Ruetti Markus , Rutz Remus , Schmid Hans Ruedi , Stocker Reto , Vuichard-Gysin Danielle , Wiggli Benedikt , Besold Ulrike , Kuster Stefan P , McGeer Allison , Risch Lorenz , Friedl Andrée , Schlegel Matthias , Schmid Dagmar , Vernazza Pietro , Kahlert Christian R , Kohler Philipp 
Published: January 2022

Abstract

Background
The burden of long-term symptoms (ie, long COVID) in patients after mild COVID-19 is debated. Within a cohort of healthcare workers (HCWs), frequency and risk factors for symptoms compatible with long COVID are assessed.

Methods
Participants answered baseline (August/September 2020) and weekly questionnaires on SARS-CoV-2 nasopharyngeal swab (NPS) results and acute disease symptoms. In January 2021, SARS-CoV-2 serology was performed; in March, symptoms compatible with long COVID (including psychometric scores) were asked and compared between HCWs with positive NPS, seropositive HCWs without positive NPS (presumable asymptomatic/pauci-symptomatic infections), and negative controls. The effect of time since diagnosis and quantitative anti-spike protein antibodies (anti-S) was evaluated. Poisson regression was used to identify risk factors for symptom occurrence.

Results
Of 3334 HCWs (median, 41 years; 80% female), 556 (17%) had a positive NPS and 228 (7%) were only seropositive. HCWs with positive NPS more frequently reported ≥1 symptom compared with controls (73% vs 52%, P &lt; .001); seropositive HCWs without positive NPS did not score higher than controls (58% vs 52%, P = .13), although impaired taste/olfaction (16% vs 6%, P &lt; .001) and hair loss (17% vs 10%, P = .004) were more common. Exhaustion/burnout was reported by 24% of negative controls. Many symptoms remained elevated in those diagnosed &gt;6 months ago; anti-S titers correlated with high symptom scores. Acute viral symptoms in weekly questionnaires best predicted long-COVID symptoms. Physical activity at baseline was negatively associated with neurocognitive impairment and fatigue scores.

Conclusions
Seropositive HCWs without positive NPS are only mildly affected by long COVID. Exhaustion/burnout is common, even in noninfected HCWs. Physical activity might be protective against neurocognitive impairment/fatigue symptoms after COVID-19.
Comparison of Intra-Familial Transmission of Influenza Virus From Index Patients Treated With Baloxavir Marboxil or Oseltamivir Using an Influenza Transmission Model and a Health Insurance Claims Database
Authors: Miyazawa Shogo , Takazono Takahiro , Hosogaya Naoki , Yamamoto Kazuko , Watanabe Hideaki , Fujiwara Masakazu , Fujita Satoki , Mukae Hiroshi 
Published: January 2022

Abstract

Background
Influenza affects approximately a billion people globally, including &gt; 10 million Japanese individuals every year. Baloxavir marboxil (baloxavir [BXM]; a selective cap-dependent endonuclease inhibitor) is approved for influenza treatment in Japan. We compared the incidence of intra-familial transmission of influenza between BXM and oseltamivir (OTV) treatments using a simulation model.

Methods
Using the JMDC Claims Database, we identified index case (IC) as the first family member diagnosed with influenza during the 2018–19 influenza season, and classified the families into BXM or OTV group per the drug dispensed to ICs. Using a novel influenza intra-familial infection model, we simulated the duration of influenza infection in ICs based on agent-specific virus shedding periods. Intra-familial infections were defined as non-IC family members infected during the agent-specific viral shedding period in ICs. The virus incubation periods in the non-IC family members were considered to exclude secondary infections from potentially external exposure. The primary endpoint was proportion of families with intra-familial infections. For between-group comparisons, we used a multivariate logistic regression model.

Results
The median proportion of families with intra-familial transmission was 9.57% and 19.35% in the BXM (N = 84 672) and OTV (N = 62 004) groups, respectively. The multivariate odds ratio of 1.73 (2.5th–97.5th percentiles, 1.68–1.77) indicated a substantially higher incidence of intra-familial infections in the OTV group versus the BXM group. Subgroup analyses by ICs’ age category, virus type, and month of onset revealed similar trends favoring BXM.

Conclusions
BXM treatment of ICs may contribute to a greater reduction in intra-familial influenza transmission than OTV treatment.
Rural Policing in the Long Trecento: An Urban Project and Its Obstruction
Authors: Geltner G 
Published: January 2022

Abstract
The intricacies of urban–rural relations surface with rare detail from the records of Italian field wardens (campari) from the late thirteenth to the mid-fifteenth century. Focusing on the region of Piedmont, in the peninsula’s north-west, this article traces the policing of numerous sites and species by the campari as part of an urban bio- and agropolitical project. The office reflects the growing desire of towns to control their surrounding countryside, not only for military defence, but also as an essential source of calorific and hydraulic energy, a sink for waste and a stage on which to showcase their power before internal and external audiences. Reconstructing the remits, norms and actions of field wardens thus illuminates power negotiations that were shaped by a range of environmental factors and the era’s thinking about hygiene. Yet analysing the activities and responsibilities of the campari also reveals the tactics that rural dwellers devised to contest urban discipline, for instance through self-help, concealment and the embellishment of charges made against them. From the perspective of the area’s historiography, the dynamics captured by these records challenge the centrality of landed aristocracies in narratives of political centralisation. And from a broader perspective, the granular view they afford of middling officials at work invites historians to explore what urbanisation meant at the ground level, on either side of the city walls.
Tricuspid valve repair concomitant with the Norwood operation among babies with hypoplastic left heart syndrome
Authors: Wamala Isaac , Friedman Kevin G , Saeed Mossab Y , Gauvreau Kimberlee , Gellis Laura , Borisuk Michele , Kaza Aditya , Emani Sitaram , del Nido Pedro J , Baird Christopher W 
Published: January 2022

Abstract

OBJECTIVES
Among patients with hypoplastic left heart syndrome (HLHS), tricuspid valve regurgitation (TR) portends a poor prognosis. Our goal was to describe the outcomes of tricuspid valve reconstruction (TVR) concomitant with the Norwood operation and using two-dimensional echocardiography and evaluate the structural factors associated with successful functional correction.

METHODS
We performed a retrospective, single-centre study of patients with HLHS undergoing TVR at the time of the Norwood operation. Structural echocardiographic parameters were compared between patients with successful correction (≤ mild TR) and those with ≥ moderate regurgitation at discharge. Preoperative dimensions of matched HLHS controls with ≤ trivial TR were used as a reference.

RESULTS
Of 205 patients with HLHS undergoing the Norwood operation, 18 patients had a concomitant TVR. Ten (56%) patients had an improved TR grade postoperatively, 8 (44%) of whom had ≤ mild TR at discharge. Improvement in TR grade (P = 0.001) and having ≤ mild TR at discharge (P = 0.011) were associated with an improved reintervention and TR-free survival. Patients with successful functional correction had smaller preoperative tricuspid annulus lateral dimensions (P = 0.023), tricuspid valve area (P = 0.007) and right ventricle mid-width (P = 0.064). Preoperatively, the successful TVR cases tended to have had higher anterior leaflet excursion (80 ± 20 vs 55 ± 11, P = 0.010), and a higher proportion of anterior leaflet prolapse (63% vs 10%, P = 0.043) compared to cases where TVR was not successful.

CONCLUSIONS
Patients with HLHS with significant tricuspid regurgitation undergoing the stage 1 operation were more likely to have successful concomitant tricuspid valve repair if they had less tricuspid annular dilation, less-severe RV enlargement and predominantly anterior leaflet prolapse. Successful tricuspid valve repair was associated with improved mid- and long-term outcomes.
What Drives Anti-Immigrant Sentiments Online? A Novel Approach Using Twitter
Authors: Menshikova Anastasia , van Tubergen Frank 
Published: January 2022

AbstractMost studies use survey data to study people’s prejudiced views. In a digitally connected world, research is needed on out-group sentiments expressed online. In this study, we show how one can elaborate on existing sociological theories (i.e. group threat theory, contact theory) to test whether anti-immigrant sentiments expressed on Twitter are related to sociological conditions. We introduce and illustrate a new method of collecting data on online sentiments, creating a panel of 28,000 Twitter users in 39 regions in the United Kingdom. We apply automated text analysis to quantify anti-immigrant sentiments of 500,000 tweets over a 1-year period. In line with group threat theory, we find that people tweet more negatively about immigrants in periods following more salient coverage of immigration in the news. We find this association both for national news coverage, and for the salience of immigration in the personalized set of outlets people follow on Twitter. In support of contact theory, we find evidence to suggest that Twitter users living in areas with more non-western immigrants, and those who follow a more ethnically diverse group of people, tweet less negatively about immigrants.
Understanding the pathways leading to gut dysbiosis and enteric environmental dysfunction in infants: the influence of maternal dysbiosis and other microbiota determinants during early life
Authors: Moya-Alvarez Violeta , Sansonetti Philippe J 
Published: January 2022

AbstractMaternal environmental enteric dysfunction (EED) encompasses undernutrition with an inflammatory gut profile, a variable degree of dysbiosis and increased translocation of pathogens in the gut mucosa. Even though recent research findings have shed light on the pathological pathways underlying the establishment of the infant gut dysbiosis, evidence on how maternal EED influences the development of gut dysbiosis and EED in the offspring remains elusive. This review summarizes the current knowledge on the effect of maternal dysbiosis and EED on infant health, and explores recent progress in unraveling the mechanisms of acquisition of a dysbiotic gut microbiota in the offspring. In Western communities, maternal inoculum, delivery mode, perinatal antibiotics, feeding practices and infections are the major drivers of the infant gut microbiota during the first 2 years of life. In other latitudes, the infectious burden and maternal malnutrition might introduce further risk factors for infant gut dysbiosis. Novel tools, such as transcriptomics and metabolomics, have become indispensable to analyze the metabolic environment of the infant in utero and postpartum. Human milk oligosaccharides have essential prebiotic, antimicrobial and anti-biofilm properties that might offer additional therapeutic opportunities.
A Chromosome-Level Genome Assembly of the Parasitic Wasp Chelonus formosanus Sonan 1932 (Hymenoptera: Braconidae)
Authors: Liu Jian-Feng , Zhao Hai-Yan , Song Yan-Fei , Yu Yuan-Chan , Yang Mao-Fa 
Published: January 2022

Abstract
Chelonus formosanus Sonan 1932 (Hymenoptera: Braconidae) is a wasp capable of parasitizing a variety of lepidopteran pests at the “egg-larval” stage which distributes throughout Taiwan, Guangdong, Zhejiang, and Hainan provinces of China. This wasp has been successfully used to control pests such as Spodoptera litura Fabricius, 1775, Spodoptera frugiperda (JE Smith, 1797), Spodoptera exigua (Hübner, 1808), and Helicoverpa armigera (Hübner, 1808). So far, there is only one genome assembled from the Chelonus genus [Chelonus insularis (Cresson, 1865)] and it is fragmented with 455 scaffolds. Here, we report a chromosome-level genome assembly of C. formosanus, which was sequenced using PacBio, Illumina, and Hi-C technologies. The long reads were 35.4 Gb (∼150× coverage) with an average length of 15.23 kb. The size of the genome assembly was 139.59 Mb. More than 99.46% of the assembled sequences were anchored to seven pseudochromosomes (138.84 Mb). The Benchmarking University Single-Copy Orthologs (BUSCO) assessment results showed 99.0% of the 1,367 genes (insect_odb10 database) were completely present. We annotated 11,242 protein-coding genes including 98.6% of BUSCO complete genes that were recovered. Nearly one-fourth of the genome assembly (22.25%) was annotated as repetitive sequences and 324 noncoding RNAs were predicted. There were 58 gene families found with significant expansion including allelopathic families (odorant receptors and ionotropic receptors), which may play a crucial role in efficiently locating a wide range of hosts. This high-quality genome assembly and annotation could provide a highly valuable resource of parasitic wasp for the biological control of Lepidoptera pest.
Characteristics of the North Anatolian Fault at the eastern end of Marmara seismic gap based on multidisciplinary field evidence
Authors: Kozacı Özgür , Altunel Erhan 
Published: January 2022

SUMMARYThe northern branch of the North Anatolian Fault (NAF) is the primary source of the greatest natural hazard for the Marmara Megapolis and specifically the İstanbul Metropolitan area. The Sea of Marmara section of the NAF is a ∼125-km-long seismic gap bound by the M7.4 1912 Mürefte earthquake rupture in the west and M7.4 1999 İzmit earthquake rupture in the east. Terminations of these ruptures define the length of the seismic gap in the Marmara Sea and consequently the magnitude and rupture extent of the expected large magnitude earthquake. The Hersek Peninsula is the last location where the NAF can be studied on land before it enters the Sea of Marmara. In addition, it is a geologically key location for the western termination of the M7.4 İzmit surface rupture. This study integrates high-resolution onshore and offshore data from Hersek Peninsula and its vicinity in İzmit Bay to assess the seismic hazard for the Marmara region. Detailed geological and geomorphic mapping, palaeoseismic trenching, geophysical and geotechnical subsurface data and archeoseismologic investigations demonstrate that the 1999 İzmit earthquake surface rupture died off east of the Hersek Peninsula due to structural complexity of the NAF at this location and the most recent earthquake to rupture the surface at Hersek Peninsula was the historical 1509 CE event. A restraining bend and bifurcation of the NAF at Hersek Peninsula suggests that this location might be a persistent asperity forming a surface rupture segmentation point at least since Pleistocene. Surface ruptures documented in palaeoseismic trenches north of the Hersek Lagoon and an offset 6th century East Roman aqueduct; however, suggests that this section of the NAF has ruptured the surface multiple times in the past and is highly likely to rupture again during the expected Marmara earthquake. The aqueduct; which crosses the surface trace of the NAF, is offset 14 ± 1 m and yields 13.6 ± 1 mm yr–1 dextral slip rate indicating that the northern branch of the NAF accrues and releases the majority of the stress between Eurasia and Anatolian block in the Marmara region. If the ∼125-km-long seismic gap between the 1912 and 1999 ruptures fail during a single event the expected Marmara earthquake can reach M7.4.
Extraction of GRACE/GRACE-FO observed mass change patterns across Antarctica via independent component analysis (ICA)
Authors: Shi Tianyan , Fukuda Yoichi , Doi Koichiro , Okuno Jun’ichi 
Published: January 2022

SUMMARY
Here we qualitatively analyse the mass change patterns across Antarctica via independent component analysis (ICA), a statistics-based blind source separation method to extract signals from complex data sets, in an attempt to reduce uncertainties in the glacial isostatic adjustment (GIA) effects and improve understanding of Antarctic Ice Sheet (AIS) mass-balance. We extract the six leading independent components from gravimetric data acquired during the Gravity Recovery and Climate Experiment (GRACE) and GRACE Follow-On (GRACE-FO) missions. The results reveal that the observed continental-scale mass changes can be effectively separated into several spatial patterns that may be dominated by different physical processes. Although the hidden independent physical processes cannot be completely isolated, some significant signals, such as glacier melt, snow accumulation, periodic climatic signals, and GIA effects, can be determined without introducing any external information. We also observe that the time period of the analysed data set has a direct impact on the ICA results, as the impacts of extreme events, such as the anomalously large snowfall events in the late 2000s, may cause dramatic spatial and temporal changes in the ICA results. ICA provides a unique and informative approach to obtain a better understanding of both AIS-scale mass changes and specific regional-scale spatiotemporal signal variations.