bluuebunny/crossref_metadata_2025_split huggingface dataset:
Participatory interventions for collective action and sustainable resource management: linking actors, situations and contexts through the IAD, NAS and SES frameworks
Authors: Ortiz-Riomalo Juan Felipe , Koessler Ann-Kathrin , Miranda-Montagut Yaddi , Cardenas Juan Camilo 
Published: September 2022

AbstractOvercoming complex environmental challenges demands different forms of stakeholder participation and collective action. While informative and relevant for participatory interventions, the literatures on collective action and participatory governance have largely remained disconnected. We illustrate how the institutional analysis and development (IAD), network of (adjacent) action situation (NAS) and social–ecological system (SES) frameworks can be combined to provide a coherent approach that integrates these literatures, applies their insights and bridges this disconnect. We compare two similar participatory interventions, one in Colombia and one in Peru, whose design and implementation we supported. Transdisciplinary in nature, both sought to foster collective action for watershed management. The frameworks allow us to demarcate, characterise and reflect upon the action situations (ASs) for the collective choice, coordination and knowledge generation that constituted each participatory intervention (i.e. the constituent NAS) and other relevant operational and institutional ASs that lay outside the boundaries of the participatory interventions. These other ASs may not be linked to one another or to the intervention’s constituent NAS, but they influence the outcomes of interest nevertheless, thereby shaping the potential of the participatory interventions for collective action and sustainable natural resource management. The framework then suggests, and our comparative analysis illustrates, that organisers and researchers of participatory interventions, such as multi-actor deliberative platforms and transdisciplinary research projects, should carefully consider, reflect upon and address the constellation of relevant actors, ASs and contexts co-determining the outcomes of interest. Our study demonstrates how the IAD, SES and NAS frameworks can support that endeavour.
Irisin promotes the proliferation and tenogenic differentiation of rat tendon-derived stem/progenitor cells via activating YAP/TAZ
Authors: Xu Langhai , Chen Zhonggai , Geng Tingting , Ru Bin , Wan Quan , Zhang Jianbin , Li Shun , Cai Wenjun 
Published: September 2022

AbstractTendinopathy is a common tendon disorder characterized by pain, swelling, and dysfunction. Current evidence has demonstrated that the depletion of stem cell pool and non-tenogenic differentiation of tendon-derived stem/progenitor cells (TSPCs) might account for the pathogenesis of tendinopathy. FNDC5/Irisin, as a novel exercise-induced myokine, is proved to be involved in the exercise-induced protective effects on musculoskeletal disorders. However, whether irisin can affect TSPCs fate is still unknown. To ascertain the roles of irisin on the proliferation and tenogenic differentiation of TSPCs, rat TSPCs were isolated and incubated with irisin. Cell viability, phenotypic changes, and related signaling pathways were evaluated by CCK-8 assay, colony formation assay, real-time PCR, Western blot, immunofluorescence, and proteasome activity assay. We found that irisin treatment increased the proliferative and colony-forming abilities, and promoted the tenogenic differentiation of TSPCs by upregulating the expression of YAP/TAZ. In conclusion, our work showed for the first time that irisin promotes the proliferation and tenogenic differentiation of rat TSPCs in vitro by activating YAP/TAZ, and the process was associated with a ubiquitin-proteasome proteolytic pathway. In conclusion, irisin and agents targeting YAP/TAZ may be promising therapeutic options for tendinopathy.
A new empirical model for predicting flue gas miscibility for light oils
Authors: Nekekpemi Prosper , Boukadi Fathi , Olayiwola Olatunji 
Published: September 2022

AbstractIt is typical to observe a decline in production rate and a decrease in reservoir pressure after oil reservoirs have been allowed to produce for a long time. Miscible flooding is a tertiary recovery method for enhancing the reservoirs’ sweep efficiency. During miscible injection, gasses such as carbon dioxide, natural gas, and nitrogen are employed to increase production levels. Carbon dioxide is commonly used as a miscible gas, but less abundant and more expensive than nitrogen. Flue gas, a mixture of carbon dioxide and nitrogen gas, is often used to replace pure carbon dioxide. For this study, flue gasses with the compositions, 15% of carbon dioxide, 85% of nitrogen gas and 30% of carbon dioxide,70% of nitrogen gas, are used as the injection gas to develop an empirical correlation for minimum miscibility pressure (MMP) for candidate light oil reservoirs that have been previously waterflooded. In data analysis and data analytics, the dataset was separated into two groups at random: the training set, which consists of 80% of the entire dataset, and the testing set, which made up 20% of the total dataset. The independent variables employed for model development include temperature, oil sample oil gravity, molecular percentage of carbon dioxide in the injection gas, the molecular weight of hexane plus in the oil, and the molecular percentage of intermediates. The findings reveal that the newly built model is more accurate and delivers better predictions than the existing correlations. For the testing dataset, the new model predicts flue gas MMP with an average absolute percentage error of 5.5519% and a correlation coefficient of 0.92.
Wahlrechtsreform: Ist der gordische Knoten endlich geplatzt?
Authors: Behnke Joachim 
Published: September 2022

ZusammenfassungSeit Anfang der 1990er Jahre gibt es eine Debatte über eine notwendige Reform des Wahlrechts. Anlass war die sprunghaft gestiegene Anzahl sogenannter Überhangmandate. Fast alle in der dreißigjährigen Debatte vorgebrachten Vorschläge lassen sich danach charakterisieren, welche von drei grundlegenden Bedingungen sie aufzugeben oder einzuschränken bereit sind, um die Regelgröße des Bundestags weitgehend einzuhalten. Diese drei Bedingungen sind: Die Aufrechterhaltung des Interparteienproporzes, die Unantastbarkeit der Wahlkreismandate und die Unantastbarkeit der Listenmandate. Mit der Wahlgesetzänderung von 2013 wurde die Einführung von Ausgleichsmandaten beschlossen, die den durch die Überhangmandate verletzten Proporz zwischen den Parteien wiederherstellen sollten. Allerdings führten diese zu einer extremen Vergrößerung des Bundestags bei den folgenden Wahlen. Maßnahmen zur Dämpfung des Vergrößerung im Wahlgesetzes von 2020 erwiesen sich als weitgehend wirkungslos. Das Wahlgesetz von 2020 sah des weiteren die Einsetzung einer Wahlrechtskommission vor, die über effektive Maßnahmen zur Verkleinerung des Bundestags beraten sollte. Im Sommer 2022 liegen nun zwei Vorschläge aus dem Kontext der Kommission vor. Der Vorschlag der Ampel sieht vor, die Regelgröße strikt einzuhalten, indem nur soviele Wahlkreismandate an die Parteien in den einzelnen Bundesländern zugeteilt werden, wie ihnen dort nach den Zweitstimmen zustehen. Die personale Besetzung der Wahlkreismandate soll durch eine Ersatzstimmenkonstruktion gewährleistet sein. Der Vorschlag der Unionsparteien sieht einen grundlegenden Systemwechsel zu einem Grabenwahlsystem vor. Soll Fairness eine weitere Bedingung an den Reformprozess sein, dann erfüllt nur der Ampelvorschlag die entsprechenden Anforderungen.
Systematische Literaturanalyse zum KI-Einsatz und KI-basierten Geschäftsmodellen in produzierenden kleinen und mittleren Unternehmen
Authors: Merkel-Kiss Mirjam , von Garrel Jörg 
Published: September 2022

ZusammenfassungDie Arbeitswelt befindet sich im Umbruch und intensiviert die Debatte zum Thema „Zukunft der Arbeit“, vor allem zu den Möglichkeiten und Auswirkungen künstlicher Intelligenz. Der Beitrag stellt die Ergebnisse einer systematischen, datenbankbasierten Literaturanalyse zum aktuellen Stand des Einsatzes von Künstlicher Intelligenz in kleinen und mittleren Unternehmen dar. Vorgestellt werden die in den identifizierten empirischen Studien genannten Vorteile des Einsatzes von KI, die genutzten Technologien sowie Hemmnisse bei der Nutzung bzw. Einführung von KI in KMU. Ergebnis der Literaturanalyse ist, dass kaum empirische Untersuchungen zur Nutzung von KI speziell in KMUs vorhanden sind.Praktische Relevanz: Dieser Artikel stellt nicht nur den Anteil der Unternehmen, welche bereits Systeme Künstlicher Intelligenz einsetzen dar, sondern auch deren Vorteile, Hinderungsgründe sowie mögliche Einsatzgebiete in kleineren und mittleren Unternehmen. Die im Rahmen des Projekts entstehende Wissensplattform WiWiEn soll darüber hinaus Unternehmen in die Lage versetzen, selbst entsprechende KI-basierende Systeme im eigenen Unternehmen zu etablieren.
Distinctive phosphorylation pattern during mitotic exit network (MEN) regulation is important for the development and pathogenicity of Magnaporthe oryzae
Authors: Feng Wanzhen , Wang Jiansheng , Liu Xinyu , Wu Haowen , Liu Muxing , Zhang Haifeng , Zheng Xiaobo , Wang Ping , Zhang Zhengguang 
Published: September 2022

AbstractThe mitotic exit network (MEN) pathway is a vital kinase cascade regulating the timely and correct progress of cell division. In the rice blast fungus Magnaporthe oryzae, the MEN pathway, consisting of conserved protein kinases MoSep1 and MoMob1-MoDbf2, is important in the development and pathogenicity of the fungus. We found that deletion of MoSEP1 affects the phosphorylation of MoMob1, but not MoDbf2, in contrast to what was found in the buddy yeast Saccharomyces cerevisiae, and verified this finding by in vitro phosphorylation assay and mass spectrometry (MS) analysis. We also found that S43 residue is the critical phosphor-site of MoMob1 by MoSep1, and proved that MoSep1-dependent MoMob1 phosphorylation is essential for cell division during the development of M. oryzae. We further provided evidence demonstrating that MoSep1 phosphorylates MoMob1 to maintain the cell cycle during vegetative growth and infection. Taken together, our results revealed that the MEN pathway has both distinct and conservative functions in regulating the cell cycle during the development and pathogenesis of M. oryzae.
Suppression of the insect cuticular microbiomes by a fungal defensin to facilitate parasite infection
Authors: Hong Song , Sun Yanlei , Chen Haimin , Wang Chengshu 
Published: September 2022

Abstract
Insects can assemble defensive microbiomes on their body surfaces to defend against fungal parasitic infections. The strategies employed by fungal pathogens to combat host cuticular microbiotas remains unclear. Here, we report the identification and functional characterization of the defensin-like antimicrobial gene BbAMP1 encoded by the entomopathogenic fungus Beauveria bassiana. The mature peptide of BbAMP1 can coat fungal spores and can be secreted by the fungus to target and damage Gram-positive bacterial cells. Significant differences in insect survival were observed between the wild-type and BbAMP1 mutant strains during topical infection but not during injection assays that bypassed insect cuticles. Thus, BbAMP1 deletion considerably reduced fungal virulence while gene overexpression accelerated the fungal colonization of insects compared with the wild-type strain in natural infections. Topical infection of axenic Drosophila adults evidenced no difference in fly survivals between strains. However, the gnotobiotic infections with the addition of Gram-positive but not Gram-negative bacterial cells in fungal spore suspensions substantially increased the survival of the flies treated with ∆BbAMP1 compared to those infected by the wild-type and gene-overexpression strains. Bacterial colony counts and microbiome analysis confirmed that BbAMP1 could assist the fungus to manipulate insect surface bacterial loads. This study reveals that fungal defensin can suppress the host surface defensive microbiomes, which underscores the importance to extend the research scope of fungus-host interactions.
Emerging roles of ferroptosis in cardiovascular diseases
Authors: Wang Kai , Chen Xin-Zhe , Wang Yun-Hong , Cheng Xue-Li , Zhao Yan , Zhou Lu-Yu , Wang Kun 
Published: September 2022

AbstractThe mechanism of cardiovascular diseases (CVDs) is complex and threatens human health. Cardiomyocyte death is an important participant in the pathophysiological basis of CVDs. Ferroptosis is a new type of iron-dependent programmed cell death caused by excessive accumulation of iron-dependent lipid peroxides and reactive oxygen species (ROS) and abnormal iron metabolism. Ferroptosis differs from other known cell death pathways, such as apoptosis, necrosis, necroptosis, autophagy and pyroptosis. Several compounds have been shown to induce or inhibit ferroptosis by regulating related key factors or signalling pathways. Recent studies have confirmed that ferroptosis is associated with the development of diverse CVDs and may be a potential therapeutic drug target for CVDs. In this review, we summarize the characteristics and related mechanisms of ferroptosis and focus on its role in CVDs, with the goal of inspiring novel treatment strategies.
Structural insights into cyanobacterial RuBisCO assembly coordinated by two chaperones Raf1 and RbcX
Authors: Li Qiong , Jiang Yong-Liang , Xia Ling-Yun , Chen Yuxing , Zhou Cong-Zhao 
Published: September 2022

AbstractRuBisCO is the most abundant enzyme in nature, catalyzing the fixation of CO2 in photosynthesis. Its common form consists of eight RbcL and eight RbcS subunits, the assembly of which requires a series of chaperones that include RbcX and RuBisCO accumulation factor 1 (Raf1). To understand how these RuBisCO-specific chaperones function during cyanobacterial RbcL8RbcS8 (L8S8) holoenzyme formation, we solved a 3.3-Å cryo-electron microscopy structure of a 32-subunit RbcL8Raf18RbcX16 (L8F8X16) assembly intermediate from Anabaena sp. PCC 7120. Comparison to the previously resolved L8F8 and L8X16 structures together with biochemical assays revealed that the L8F8X16 complex forms a rather dynamic structural intermediate, favoring RbcS displacement of Raf1 and RbcX. In vitro assays further demonstrated that both Raf1 and RbcX function to regulate RuBisCO condensate formation by restricting CcmM35 binding to the stably assembled L8S8 holoenzymes. Combined with previous findings, we propose a model on how Raf1 and RbcX work in concert to facilitate, and regulate, cyanobacterial RuBisCO assembly as well as disassembly of RuBisCO condensates.
Silicone oil residual after vitrectomy for rhegmatogenous retinal detachment
Authors: Zhao Hongmei , Cheng Tongjie , Wu Kaicheng , Yu Jian , Zong Yuan , Chen Qian , Jiang Chunhui , Zhu Haohao , Xu Gezhi 
Published: September 2022

Abstract
Objective
To explore the presence of residual emulsified silicone oil (SO) droplets in patients with rhegmatogenous retinal detachment (RRD) and their possible risk factors.

Methods
Patients who underwent primary pars plana vitrectomy with SO injection for RRD and SO removal at the same eye centre were included. Approximately 10 weeks after SO removal, B-scan ultrasonography was performed, and using ImageJ, the silicone oil index (SOI) was measured, and its possible correlations with other clinical factors were explored.

Results
A total of 101 eyes were included. Residual SO particles were found in all the patients (100%), and the mean SOI was 4.04% ± 5.16% (range 0.06%–19.88%). Multiple linear regression revealed that, among all the clinical factors, axial length (AL) and ocular hypertension (intraocular pressure (IOP) > 21 mmHg or the use of antiglaucoma medications) before SO removal were positively and significantly associated with the SOI (all P < 0.05). Patients with ocular hypertension after SO removal had a higher SOI, a longer SO duration, a higher IOP before SO removal and a longer AL than those without (all P < 0.05).

Conclusions
Patients with a larger AL and higher IOP before SO removal were more prone to have more residual SO droplets, which might in turn lead to an elevated IOP. In these eyes, thorough irrigation or repeated fluid-air exchange might be necessary.
Apatinib plus vinorelbine versus vinorelbine for metastatic triple-negative breast cancer who failed first/second-line treatment: the NAN trial
Authors: Li Dou-Dou , Tao Zhong-hua , Wang Bi-Yun , Wang Lei-Ping , Cao Jun , Hu Xi-Chun , Zhang Jian 
Published: September 2022

AbstractWhile therapies such as chemotherapy combined with immunotherapy, sacituzumab govitecan, and PARP inhibitors are available for metastatic TNBC, on disease progression after these therapies, the mainstay of therapy is chemotherapy. Apatinib is a small-molecule tyrosine kinase inhibitor that has promising anti-angiogenesis and antitumor activity for TNBC. We aimed to evaluate the safety and efficacy of adding apatinib to chemotherapy in patients with advanced TNBC with failed first/second-line treatment. A total of 66 patients were randomly assigned, in a 1:1 ratio, to receive vinorelbine or vinorelbine with apatinib in 28-day cycles. The primary endpoint was progression-free survival (PFS). Secondary endpoints included overall survival (OS), overall response rate (ORR) and safety. 33 received apatinib plus vinorelbine and 32 received vinorelbine (1 was withdrawal). Median PFS was significantly longer in the apatinib plus vinorelbine group than in the vinorelbine group (3.9 months vs. 2.0 months; hazard ratio, 1.82; 95% confidence interval [CI], 1.06 to 3.11; P = 0.026). Median OS was 11.5 months with apatinib plus vinorelbine and 9.9 months with vinorelbine (HR,1.01; 95% CI, 0.51 to 1.97; P = 0.985). The ORR was 9.1% in the apatinib plus vinorelbine group and 6.3% in the vinorelbine group (P = 0.667). The most common treatment-related hematologic grade 3–4 adverse events in apatinib plus vinorelbine group, were leukopenia, granulocytopenia, anemia, and thrombocytopenia. no treatment-related nonhematologic grade 4 adverse events or treatment-related deaths were observed. Collectively, adding apatinib to vinorelbine shows a promising benefit in PFS compared to vinorelbine monotherapy, with an excellent toxicity profile, warranting further exploration.
Haploinsufficiency of the lysosomal sialidase NEU1 results in a model of pleomorphic rhabdomyosarcoma in mice
Authors: Machado Eda R. , van de Vlekkert Diantha , Sheppard Heather S. , Perry Scott , Downing Susanna M. , Laxton Jonathan , Ashmun Richard , Finkelstein David B. , Neale Geoffrey A. , Hu Huimin , Harwood Frank C. , Koo Selene C. , Grosveld Gerard C. , d’Azzo Alessandra 
Published: September 2022

AbstractRhabdomyosarcoma, the most common pediatric sarcoma, has no effective treatment for the pleomorphic subtype. Still, what triggers transformation into this aggressive phenotype remains poorly understood. Here we used Ptch1+/−/ETV7TG/+/− mice with enhanced incidence of rhabdomyosarcoma to generate a model of pleomorphic rhabdomyosarcoma driven by haploinsufficiency of the lysosomal sialidase neuraminidase 1. These tumors share mostly features of embryonal and some of alveolar rhabdomyosarcoma. Mechanistically, we show that the transforming pathway is increased lysosomal exocytosis downstream of reduced neuraminidase 1, exemplified by the redistribution of the lysosomal associated membrane protein 1 at the plasma membrane of tumor and stromal cells. Here we exploit this unique feature for single cell analysis and define heterogeneous populations of exocytic, only partially differentiated cells that force tumors to pleomorphism and promote a fibrotic microenvironment. These data together with the identification of an adipogenic signature shared by human rhabdomyosarcoma, and likely fueling the tumor’s metabolism, make this model of pleomorphic rhabdomyosarcoma ideal for diagnostic and therapeutic studies.
Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer’s disease mouse models
Authors: Dejanovic Borislav , Wu Tiffany , Tsai Ming-Chi , Graykowski David , Gandham Vineela D. , Rose Christopher M. , Bakalarski Corey E. , Ngu Hai , Wang Yuanyuan , Pandey Shristi , Rezzonico Mitchell G. , Friedman Brad A. , Edmonds Rose , De Mazière Ann , Rakosi-Schmidt Raphael , Singh Tarjinder , Klumperman Judith , Foreman Oded , Chang Michael C. , Xie Luke , Sheng Morgan , Hanson Jesse E. 
Published: September 2022

AbstractMicroglia and complement can mediate neurodegeneration in Alzheimer’s disease (AD). By integrative multi-omics analysis, here we show that astrocytic and microglial proteins are increased in TauP301S synapse fractions with age and in a C1q-dependent manner. In addition to microglia, we identified that astrocytes contribute substantially to synapse elimination in TauP301S hippocampi. Notably, we found relatively more excitatory synapse marker proteins in astrocytic lysosomes, whereas microglial lysosomes contained more inhibitory synapse material. C1q deletion reduced astrocyte–synapse association and decreased astrocytic and microglial synapses engulfment in TauP301S mice and rescued synapse density. Finally, in an AD mouse model that combines β-amyloid and Tau pathologies, deletion of the AD risk gene Trem2 impaired microglial phagocytosis of synapses, whereas astrocytes engulfed more inhibitory synapses around plaques. Together, our data reveal that astrocytes contact and eliminate synapses in a C1q-dependent manner and thereby contribute to pathological synapse loss and that astrocytic phagocytosis can compensate for microglial dysfunction.
Random Forest classification of Gaia DR3 white dwarf-main sequence spectra: A feasibility study
Authors: Echeverry David , Torres Santiago , Rebassa-Mansergas Alberto , Ferrer-Burjachs Aina 
Published: September 2022

Aims. The third Gaia data release provides low-resolution spectra for around 200 million sources. It is expected that a sizeable fraction of them contain a white dwarf (WD), neither isolated, or in a binary system with a main-sequence (MS) companion, that is a white dwarf-main sequence (WDMS) binary. Taking advantage of a consolidated Random Forest algorithm used in the classification of WDs, we extend it to study the feasibility of classifying Gaia WDMS binary spectra.
Methods. The Random Forest algorithm is first trained with a set of synthetic spectra generated by combining individual WD and MS spectra for the full range of effective temperatures and surface gravities. Moreover, with the aid of a detailed population synthesis code, we simulate the Gaia spectra for the abovementioned populations. For evaluating the performance of the models, a set of metrics are applied to our classifications.
Results. Our results show that for resolving powers above ~300 the accuracy of the classification depends exclusively on the S/R of the spectra, while below that value the S/R should be increased as the resolving power is reduced to maintain a certain accuracy. The algorithm is then applied to the already classified SDSS WDMS catalog, revealing that the automated classification exhibits an accuracy comparable (or even higher) to previous classification methods. Finally, we simulate the Gaia spectra, showing that our algorithm is able to correctly classify nearly 80% the synthetic WDMS spectra.
Conclusions. Our algorithm represents a useful tool in the analysis and classification of real Gaia WDMS spectra. Even for those spectra dominated by the flux of the MS stars, the algorithm reaches a high degree of accuracy (60%).