bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the mechanism of action of licorice in the treatment of COVID-19 through bioinformatics analysis and molecular dynamics simulation
Authors: Cao Jun-Feng , Gong Yunli , Wu Mei , Yang Xingyu , Xiong Li , Chen Shengyan , Xiao Zixuan , Li Yang , Zhang Lixin , Zan Wang , Zhang Xiao 
Published: September 2022

Purpose: The rapid worldwide spread of Corona Virus Disease 2019 (COVID-19) has become not only a global challenge, but also a lack of effective clinical treatments. Studies have shown that licorice can significantly improve clinical symptoms such as fever, dry cough and shortness of breath in COVID-19 patients with no significant adverse effects. However, there is still a lack of in-depth analysis of the specific active ingredients of licorice in the treatment of COVID-19 and its mechanism of action. Therefore, we used molecular docking and molecular dynamics to explore the mechanism of action of licorice in the treatment of COVID-19.Methods: We used bioinformatics to screen active pharmaceutical ingredients and potential targets, the disease-core gene target-drug network was established and molecular docking was used for verification. Molecular dynamics simulations were carried out to verify that active ingredients were stably combined with protein targets. The supercomputer platform was used to measure and analyze stability of protein targets at the residue level, solvent accessible surface area, number of hydrogen bonds, radius of gyration and binding free energy.Results: Licorice had 255 gene targets, COVID-19 had 4,628 gene targets, the intersection gene targets were 101. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene ontology (GO) analysis showed that licorice played an important role mainly through the signaling pathways of inflammatory factors and oxidative stress. Molecular docking showed that Glycyrol, Phaseol and Glyasperin F in licorice may playe a role in treating COVID-19 by acting on STAT3, IL2RA, MMP1, and CXCL8. Molecular dynamics were used to demonstrate and analyze the binding stability of active ingredients to protein targets.Conclusion: This study found that Phaseol in licorice may reduce inflammatory cell activation and inflammatory response by inhibiting the activation of CXCL8 and IL2RA; Glycyrol may regulate cell proliferation and survival by acting on STAT3. Glyasperin F may regulate cell growth by inhibiting the activation of MMP1, thus reducing tissue damage and cell death caused by excessive inflammatory response and promoting the growth of new tissues. Therefore, licorice is proposed as an effective candidate for the treatment of COVID-19 through STAT3, IL2RA, MMP1, and CXCL8.
Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative
Authors: Mohmad Masrat , Agnihotri Nivedita , Kumar Vikas , Azam Mohammad , Wabaidur Saikh Mohammad , Kamal Raj , Kumar Rakesh , Alam Mahboob , Kaviani Sadegh 
Published: September 2022

A comprehensive aqueous phase spectrophotometric study concerning the trace level determination of iridium (III) by its reaction with benzopyran-derived chromogenic reagent, 6-chloro-3-hydroxy-7-methyl-2-(2′-thienyl)-4-oxo-4H-1-benzopyran (CHMTB), is performed. The complexing reagent instantly forms a yellow complex with Ir (III) at pH 4.63, where metal is bound to the ligand in a ratio of 1:2 as deduced by Job’s continuous variations, mole ratio, and equilibrium shift methods. The complex absorbs maximally at 413–420 nm retaining its stability for up to 4 days. An optimum set of conditions have been set with respect to the parameters governing the formation of the complex. Under the set optimal conditions, the Ir (III)-CHMTB complex coheres to Beer’s law between 0.0 and 1.5 µg Ir (III) mL−1. The attenuation coefficient and Sandell’s sensitivity are, respectively, 1.18×105 L mol−1 cm−1 and 0.00162 μg cm−2 at 415 nm. The correlation coefficient (r) and standard deviation (SD) were 0.9999 and ± 0.001095, respectively, whereas the detection limit as analyzed was 0.007437 μg ml−1. The interference with respect to analytically important cations and complexing agents has been studied thoroughly. It is found that the majority of the ions/agents do not intervene with the formation of the complex, thus adding to the versatility of the method. The results obtained from the aforesaid studies indicate a simple, fast, convenient, sensitive, and versatile method for microgram analysis of iridium (III) using CHMTB as a binding ligand. Furthermore, the studied complex is subjected to the evaluation of antibacterial and antioxidant capacity by employing the Agar Diffusion assay and DPPH. radical scavenging method, respectively. The results obtained from the mentioned assays reveal that the investigated complex possesses significant potency as an antibacterial and antioxidant agent. Finally, the computational approach through DFT of the formed complex confirmed the associated electronic properties of the studied complex.
Therapeutic effects and mechanisms of N-(9,10-anthraquinone-2-ylcarbonyl) xanthine oxidase inhibitors on hyperuricemia
Authors: Gao Tianshu , Xu Jin , Xiao Yuxiao , Li Jiaqi , Hu Weifeng , Su Xiaoyu , Shen Xudong , Yu Wan , Chen Zhen , Huang Baosheng , Li Honglei , Wang Xing 
Published: September 2022

Objective: To observe the antioxidative effects of N-(9,10-anthraquinone-2-ylcarbonyl) xanthine oxidase inhibitors (NAY) in vitro and in vivo models of hyperuricemia and explore the mechanism.Methods: A classical experimental method of acute toxicity and a chronic toxicity test were used to compare the toxic effects of different doses of NAY in mice. The hyperuricemia mouse model was established by gavage of potassium oxonate in vivo. After treatment with different doses of NAY (low dose: 10 mg/kg, medium dose: 20 mg/kg, and high dose: 40 mg/kg) and allopurinol (positive drug, 10 mg/kg), observe the levels of uric acid (UA), creatinine (CRE), and urea nitrogen (BUN) in urine and serum, respectively, and detect the activities of xanthine oxidase in the liver. The hyperuricemia cell model was induced by adenosine and xanthine oxidase in vitro. The cells were given different doses of NAY (50, 100, and 200 μmol/L) and allopurinol (100 μmol/L). Then the culture supernatant UA level of the medium was measured. The next step was to detect the xanthine oxidase activity in the liver and AML12 cells, and the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) inflammatory factors in the kidney and serum of mice. Western blot was used to detect xanthine oxidase protein expression in mouse liver tissue and AML12 cells, ASC, Caspase-1, NLRP3, GLUT9, OAT1, and OAT3 protein expression in mouse kidney tissue and HK-2 cells. Hematoxylin–eosin staining was used to stain the liver and kidney tissues of mice and observe the tissue lesions.Results: NAY had little effect on blood routine and biochemical indexes of mice, but significantly reduced the serum UA level. NAY significantly reduced the level of UA in hyperuricemia mice and cells by inhibiting xanthine oxidase activity and reduced the levels of TNF-α, IL-6, and other inflammatory factors in serum and kidney of mice. NAY can inhibit inflammation by inhibiting the NLRP3 pathway. In addition, NAY can downregulate GLUT9 protein expression and upregulate OAT1 and OAT3 protein expression to reduce the UA level by promoting UA excretion and inhibiting UA reabsorption.Conclusion: These findings suggested that NAY produced dual hypouricemic actions. On the one hand, it can inhibit the formation of UA by inhibiting xanthine oxidase inhibitors activity, and on the other hand, it can promote the excretion of UA by regulating the UA transporter. It provides new ideas for the development of hyperuricemia drugs in the future.
Effects of supplemental tannic acid on growth performance, gut health, microbiota, and fat accumulation and optimal dosages of tannic acid in broilers
Authors: Choi Janghan , Yadav Sudhir , Wang Jinquan , Lorentz Benjamin J. , Lourenco Jeferson M. , Callaway Todd R. , Kim Woo Kyun 
Published: September 2022

This study was conducted to investigate the effects of different dosages of tannic acid (TA) on growth performance, nutrient digestibility, gut health, immune system, oxidative status, microbial composition, volatile fatty acids (VFA), bone mineral density, and fat digestion and accumulation in broilers and to find optimal dosages of TA for efficient growth and gut health in broilers. A total of 320 male Cobb500 broilers were randomly distributed to 4 treatments with 8 replicates including 1) tannic acid 0 (TA0): basal diet without TA; 2) tannic acid 0.5 (TA0.5): basal diet with 0.5 g/kg TA; 3) tannic acid 1.5 (TA1.5); and 4) tannic acid 2.5 (TA2.5). Supplemental TA at levels greater than 972 mg/kg tended to reduce BW on D 21 (p= 0.05). The TA2.5 had significantly lower apparent ileal digestibility (AID) of crude protein compared to the TA0 group. The AID of ether extract tended to be reduced by TA at levels greater than 525 mg/kg (p= 0.08). The jejunal lipase activities tended to be reduced by TA at levels less than 595.3 mg/kg (p= 0.09). TA linearly decreased goblet cell density in the crypts of the jejunum (p&lt; 0.05) and reduced mRNA expression of mucin two at levels less than 784.9 mg/kg and zonula occludens two at levels less than 892.6 mg/kg (p&lt; 0.05). The TA0.5 group had higher activities of liver superoxide dismutase compared to the TA0 group (p&lt; 0.05). Bone mineral density and contents tended to be linearly decreased by TA (p= 0.05), and the ratio of lean to fat was linearly decreased (p&lt; 0.01). Total cecal VFA production tended to be linearly reduced by TA at levels greater than 850.9 mg/kg (p= 0.07). Supplemental TA tended to increase the relative abundance of the phylum Bacteroidetes (p= 0.1) and decrease the relative abundance of the phylum Proteobacteria (p= 0.1). The relative abundance of the family Rikenellaceae was the lowest at 500 mg/kg TA, and the relative abundance of the family Bacillaceae was the highest at 1,045 mg/kg TA. Collectively, these results indicate that the optimum level of supplemental TA would range between 500 and 900 mg/kg; this range of TA supplementation would improve gut health without negatively affecting growth performance in broilers under antibiotic-free conditions.
Intersection of anxiety and negative coping among Asian American medical students
Authors: Moore Michelle B. , Yang David , Raines Amanda M. , Bailey Rahn Kennedy , Beg Waania 
Published: September 2022

PurposeAsian Americans comprise 21% of matriculating medical students in the United States but little is known about their mental health. With the growing focus on addressing the mental health of medical students, this systematic, nationwide survey assesses the relationship between anxiety and depression symptoms and coping skills among Asian American medical students.Materials and methodsA survey tool comprised of Patient Health Questionnaire-9, General Anxiety Disorder-7, and questions related to coping were emailed to members of the Asian Pacific American Medical Students Association enrolled in a United States medical school during the 2016–2017 academic year. We evaluated associations between anxiety and coping as well as depression and coping.ResultsA total of 511 Asian American medical students completed the survey. Anxiety symptoms were positively correlated with an increase in negative coping skills. Depressive symptoms were not correlated with an increase in negative coping skills.ConclusionProfessionals and medical schools that aim to improve the mental health of medical students should be aware of the needs of specific populations. Asian American students who experience anxiety were more likely to utilize avoidant or negative coping strategies. In addition, Asian American students who experience depressive symptoms were not more likely to utilize these negative coping strategies. Further research must be done to evaluate the factors that influence the use of negative coping strategies to better address anxiety within the Asian American medical student population.
Mandarin Stroke Social Network Scale and Item Response Theory
Authors: Feng Chun , Lai Qing-Ling , Ferland Amanda , Lin Feng 
Published: September 2022

BackgroundStroke survivors may have difficulty in social integration due to physical or mental disorders across the entire continuum of disease. Higher perceived social support can promote social participation for persons following a stroke. However, no scale is available to investigate the perceived social support among the Chinese post-stroke population.ObjectivesThe current study addresses this gap by developing the Mandarin version of the Stroke Social Network Scale (M-SSNS) and examining the reliability and validity of this scale. This study also utilizes the Item Response Theory (IRT) model as a bridge between social ability and functional status.MethodsThe convenience sampling method was followed to recruit 71 inpatient post-stroke individuals. All participants were evaluated by the modified Barthel Index (MBI), M-SSNS, and the Extended International Classification of Functioning, Disability, and Health (ICF) Core Set for Stroke. The reliability of M-SSNS was explored based on the IRT model. The validity of the scale was further confirmed by assessing the correlation between estimated personal social competence and the final score of the 19-item M-SSNS, and the correlation between estimated personal social competence and functional status based on the ICF.ResultsA total of 71 patients with stroke (53 males and 18 females) were included in this research. Fourteen items of M-SSNS were selected to represent personal social ability according to the unidimensional 3-parameter logistic (3PL) IRT model. The Cronbach's α of the 14-item scale was 0.7192, with the Guttman's λ2 = 0.7567, Molenaar Sijtsma ρ = 0.7491, and latent class reliability coefficient = 0.8657, indicating that the M-SSNS had great internal consistency. The estimated individual social competence by the 14-item 3PL model was highly related to the final score of the 19-item M-SSNA (p &lt; 0.001, r = 0.79). The correlation between the personal functional status and social ability was 0.23 (p = 0.049, r = 0.23).ConclusionThe 14-item M-SSNS manifests great reliability and acceptable validity. Based on the IRT, the 14-item M-SSNS is also a promising tool to assess the social structure and provide customized relationship consulting, education, and advice among the Chinese stroke population.
Management of borderline ovarian tumors: A tertiary referral center experience in Egypt
Authors: Gaballa Khaled , Abdelkhalek Mohamed , Fathi Adel , Refky Basel , Belal Khaled , elaraby Moustafa , Zuhdy Mohammad 
Published: September 2022

BackgroundIn this retrospective study, we discuss our experience as a large tertiary referral center in Egypt in the management and follow-up of borderline tumorsPatients and methodsThis is a retrospective cohort study where all patients diagnosed with a borderline ovarian tumor at Oncology Center Mansoura University from November 2014 to June 2020 were included. Demographics, preoperative, operative, postoperative, pathologic, and oncologic follow-up data were retrieved from a prospectively maintained electronic database. The included patients were followed until April 2022.ResultsWe included 27 patients with borderline ovarian tumors. The mean age of the study patients was 47.67 ± 16.39 years. The median CA 125 was 33 (6–304 U/ml). Frozen section examination was utilized in 13 patients (48.14%), where a diagnosis of borderline ovarian tumors was revealed in 8 patients. Recurrence was reported in one patient with serous type after approximately 26 months. The most common pathological type in our cohort was the mucinous borderline type reported in 14 patients (51.9%), followed by the serous type reported in 11 patients (40.7%), and the seromucinous type in 1 patient only. Patients with mucinous borderline type were significantly younger (40.083 ± 18.47 vs. 53.73 ± 11.91 years, p = 0.028). Interestingly, Cancer Antigen 125 levels were significantly higher in mucinous than serous and seromucinous types [67(16–304) vs. 20(6–294.6) U/ml, p = 0.027]. On the other hand, the radiological tumor size of serous and seromucinous types was larger than that of the mucinous type [23(19–31) cm vs. 8(5–20) cm, p = 0.001]. Over a median follow-up period of 58.66 (54.16–63.16) months, only one postoperative mortality was reported, while only one recurrence was reported.ConclusionBorderline ovarian tumors still represent a dilemma either in diagnosis or management. A frozen section examination could help to reach a preliminary diagnosis. Total abdominal hysterectomy and bilateral salpingo-oophorectomy are the cornerstone of surgical management; however, fertility-sparing surgery could be a valid option for women desiring fertility.
Codification of legislation in various legal systems
Authors: Goncharova Larisa N , But'ko Lyudmila V , Dzidzoev Ruslan M , Petrishin Alexey P , Thabisimov Husen A 
Published: September 2022

This current article makes an attempt to provide a comparative analysis of the codification of legislation in various legal systems. The authors analyze the features of the systematization of legislation in the countries of the Romano-Germanic and Anglo-Saxon legal systems. Several general scientific methods and the methods of logical cognition are utilized in the study, including analysis and synthesis, systemic, functional and formal-logical approaches. Based on the results of the study, it can be concluded that it is the continental legal tradition that is most conducive to codification activity. The low volume of statutory legal norms in the Anglo-Saxon legal system impedes codification, but provides adequate regulatory flexibility. At the same time, recently in common law countries there has been a tendency to increase the share of statutory law in the regulation of public relations. In this regard, the importance of codification work increases in the systematization of legislation.
What is Queer Heritage?
Authors: Immonen Visa 
Published: September 2022

The present article explores the possibility of queer heritage. In 2017, artists Kalle Hamm and Dzamil Kamanger organised a community art project called Queer- cache in Helsinki, and I use it as a case study on the heritagisation of queer pasts. Queercache borrowed its form from geocaching, presenting twelve caches with urban stories and memories. It asked whether phenomena which deviate from norms – the rejected past – can be recognised as heritage. As a means of analys- ing heritage discourse, i.e., the process of identifying and treating something
as heritage, I use the concept of the epistemology of the closet. It describes and questions heteronormatively conditioned ways of knowing. The epistemology, and heritage discourse have commonalities, since both are processes of signification characteristic of modernity, the core of which are identification, valuation and knowing. However, Queercache nurtured queer memories without the stories necessarily meeting criteria associated with heritage such as continuity, authen- ticity and expertise.
New Authoritative Changes with Blockchain an Emphasis Production Network
Authors: Ramadhan Tarisya , Nur Wahid Wahyu 
Published: September 2022

The paper means to introduce a deliberate writing survey showing the advantages, difficulties and future examination of blockchain innovation for the store network, likewise recommending how the elements of blockchain innovation can change the hierarchical parts of the store network. This paper appears through 31 factors ordered into positive, negative and future headings of innovation for the supply network. For example, blockchain innovation will diminish tedious of tasks the board and installments utilizing brilliant agreements. Moreover, incorporating blockchain innovation with different advances will permit item following and maintainable creation of the board. The choice of papers is restricted to supply networkopus data set and explicitly to the Administration Diary. Practical implications – blockchain innovation makes cooperative shared and business-to-business markets. The innovation mechanizes a few assignments, for example, request the board, installment for products, squander decrease and interaction control. In this manner, its utilization inside the supply networks will work on the usefulness and benefits of the members. This paper is centered around blockchain innovation for the supply network region with 60 articles examined. Likewise, 13 factors on benefits, eight factors on difficulties and 12 focuses on future examination bearings were investigated. This work will help specialists and business visionaries to extend about the progressions that blockchain innovation offers in supply network.
KINERJA PEMERINTAH DAERAH DI MATA PUBLIK: STUDI KASUS PENANGANAN PANDEMI COVID-19 DI BALI TAHUN 2020
Authors: Kadek Dwita Apriani  
Published: September 2022

Bali is one of the region in Indonesia experiencing an upmost severe impact of Covid-19 pandemy. Policies formulation and implementation were done in unideal condition. The evaluations from the public opinion are really needed. This study captures the public's response over the policy of pandemic handling in Bali which is focused on the aspects of: (1) health, (2) tourism economy, and (3) social welfare. The method used in this study is survey conducted on 1.540 respondents spread all over Bali. The margin of error is 3% at the confidence level of 95%. The results showed that public values the performance of Bali Government is lower than national scores (66%). Good satisfaction numbers are shown over the health aspect (62%). Balinese rate over economic and public welfare sectors are valued as 42%. This research also found that public's response over Bali Province government policies in pandemic handling are related with the pandemic impact on respondents household financial situations.
Singing Beat Tracking With Self-supervised Front-end and Linear Transformers
Authors: Heydari Mojtaba , Duan Zhiyao 
Published: September 2022

<p>Tracking beats of singing voices without the presence of musical accompaniment can find many applications in music production, automatic song arrangement, and social media interaction. Its main challenge is the lack of strong rhythmic and harmonic patterns that are important for music rhythmic analysis in general. Even for human listeners, this can be a challenging task. As a result, existing music beat tracking systems fail to deliver satisfactory performance on singing voices. In this paper, we propose singing beat tracking as a novel task, and propose the first approach to solving this task. Our approach leverages semantic information of singing voices by employing pre-trained self-supervised WavLM and DistilHuBERT speech representations as the front-end and uses a self-attention encoder layer to predict beats. To train and test the system, we obtain separated singing voices and their beat annotations using source separation and beat tracking on complete songs, followed by manual corrections. Experiments on the 741 separated vocal tracks of the GTZAN dataset show that the proposed system outperforms several state-of-the-art music beat tracking methods by a large margin in terms of beat tracking accuracy. Ablation studies also confirm the advantages of pre-trained self-supervised speech representations over generic spectral features.</p>