bluuebunny/crossref_metadata_2025_split huggingface dataset:
CST1, as a prognostic biomarker for gastric cancer, is associated with tumor immune cells infiltration, invasion, and cell migration
Authors: Wei Jianming , Wei Zhiwang , Gao Xibo , Lu Li , Liu Tong 
Published: March 2022

Abstract
Background CST1 , acting as an oncogene, plays a critical role in tumor microenvironment (TME) remodeling. However, the function of CST1 in gastric cancer (GC) remains elusive. The purpose of this study was to explore the prognostic significance, tumor immune cell infiltration and biological functions of CST1 as a novel therapeutic candidate biomarker for GC.Methods Differentially expressed genes (DEGs) were identified using the library (“limma”) package of R software. Gene Set Enrichment Analysis (GSEA) was applied to explore the molecular function of CST1 . CST1 expression in GC cell lines was detected using quantitative real-time PCR (RT-qPCR) in vitro. Cell proliferation, migration, and invasion were measured using the cell counting kit-8 assay and Transwell assay.Results A total of 142 common DEGs were screened out in four datasets. Four overlapped DEGs ( CST1 , CLDN4 , CLDN7 and CLDN1 ) were identified and validated in TCGA database. CST1 was the most significant to be selected for further study. Bioinformatics analysis revealed that CST1 expression was associated with in tumor immune cell infiltration and clinical factors in GC. The survival analysis showed that CST1 overexpression predicted poor outcome in GC patients. In Cancer Cell Line Encyclopedia (CCLE) database, the expression of SULT1C4 , PAGE4 and FPR3 was significant positively related to CST1 . CST1 was also upregulated in GC cells AGS by qRT-PCR. Finally, we found that CST1 promoted cell proliferation, invasion, and migration in GC.Conclusion This study reveals that CST1 is a promising candidate biomarker for prognosis and potential therapy target for GC.
Diversity and population structure of Nordic potato cultivars and breeding clones grown under long daylength in Europe
Authors: Selga Catja , Chrominski Pawel , Carlson-Nilsson Ulrika , Andersson Mariette , Chawade Aakash , Ortiz Rodomiro 
Published: March 2022

Abstract
The genetic diversity and population structure of breeding germplasm is central knowledge for crop improvement. To gain insight into the genetic potential of the germplasm used for potato breeding in a Nordic breeding program as well as all av available accessions from the Nordic genebank (NordGen), 133 potato genotypes were genotyped using the Infinium Illumina 20K SNP array. After SNP filtering, 11 610 polymorphic SNPs were included in the analysis. In addition, data from three important breeding traits – percent dry matter and uniformity of tuber shape and eye – were scored to measure the variation be-tween groups. The genetic diversity among the genotypes was estimated using principal coordinate analysis based on the genetic distance between individuals, as well as by using the software STRUCTURE. Both methods suggest that the collected breeding material and the germplasm from the gene-bank are closely related, with a low degree of population structure between the groups. The phenotypic distribution among the genotypes revealed significant differences, especially between farmer’s cultivars and released cultivars and breeding clones. The percent heterozygosity was similar between the groups, with a mean average of 58–60%. Overall, the breeding germplasm and the accessions from the Nordic genebank seems to be closely related with similar genetic background. This gains insight that the genetic potential of available Nordic potato breeding germplasm is low, and for genetic hybridization purposes, genotypes from outside the Nordic region should be employed.
A Social-Ecological Perspective for Emerging Cities: The Case of the Corniche Promenade, ‘Urban Majlis’ of Doha
Authors: Furlan Raffaello , Grosvald Michael 
Published: March 2022

Abstract
In the past decade the capital city of Doha of the State of Qatar has experienced rapid urban expansion and other changes due to globalization, which has caused (i) the loss of a compact urban pattern, (ii) landscape fragmentation, and (iii) a deficiency of green spaces. Therefore, as envisioned by Qatar National Vision 2030 (QNV-2030), the State of Qatar plans to invest substantial funds into the urban regeneration of the built environment, along with the development of large areas of public parks as a means of promoting more sustainable urban development and enhancing city residents’ well-being. Accessibility contributes to the usability of public facilities on the part of the neighborhood community, thus enhancing city dwellers’ well-being. Nonetheless, the authors argue, the urban network along Doha’s Corniche promenade, the dominant open public space and spine of the city, lacks connectivity at various scales of space. Therefore, this research study aims to assess the existing conditions of Doha’s Corniche and recommends strategies for implementing its integration into the newly emerging city’s urban fabric. The findings, revealed through a network-analysis investigation based on graph theory, allows us to generate a framework for shaping open public spaces, promoting higher living standards through a green-network system planned at the city scale. The proposed framework addresses social-ecological challenges of the distinctive public open space and helps define an approach for (i) tailoring the accessibility of public open spaces to their surroundings, and (ii) enhancing city dwellers’ well-being.
The Health Education Packages using Android Applications to Improve Knowledge and Attitudes in Pregnant, Post-partum and Lactating Women in Preventing COVID-19 Transmission and Self-Care.
Authors: Setyowati Setyowati , Afiyanti Yati , Nurahmawati Imami , Sari Erna Puspita 
Published: March 2022

Abstract
Background: The Covid-19 outbreak is rapidly increasing as evidenced by the number of cases, deaths and countries affected. However, limited data is available on pregnant women with Covid-19 on which to base recommendations for pregnancy and post-partum care. Study aims: identify the effectiveness of nursing intervention with health education via an android application about self-care and prevention of Covid-19 transmission toward the knowledge and attitudes of pregnant, post-partum and lactating women. Method: This study was done in three stages. Stage one was identifying the existing nursing intervention for respondents with Covid-19, stage two involved the health education formulation and built it in to an android application, stage three measured the differences in knowledge attitude of respondents before and after health education by using the android application. 152 respondents pregnant, post-partum and lactating with Covid-19 women participated in this study. Result: Stage one showed that nursing intervention has been done comprehensively. Stage two established the health education: http://cegahcovidbumil.000webhostapp.com ; Stage three found that there were differences between pre and post health education via an android application in the knowledge and attitudes during pregnancy, post-partum and lactating women in self-care and prevention of Covid-19 transmission. Significancy was found among post-partum and lactating women (p=0.018*). Conclusion: There was a difference in knowledge and attitudes among respondents in self-care and prevention regarding the spread of Covid-19 before and after health education via an android application. Study recommended that health care providers use this method in their intervention to improve health status and prevent further spread of Covid-19. Further research to explore more health indicators is needed.
Factors Associated With Pharmacological And Non-Pharmacological Treatments Adherence In Patients With Borderline Personality Disorder In Iran
Authors: Mohammadabadi Motahareh Sadat Mirhaj , Mohammadsadeghi Homa , Adrebili Mehrdad Eftekhar , Kolour Zahra Partovi , Kashaninasab Fatemeh , Shalbafan Mohammadreza 
Published: March 2022

Abstract
Background: Borderline personality disorder is a major mental illness characterized by a sustained relationship instability, impulsive behavior and intense affects. Adherence is a complex behavior, from minor refusals of treatment to inappropriate use of health services or even abandonment of treatment, which can be affected by various factors. Therefore, the present study aimed to investigate the factors affecting pharmacological and non-pharmacological adherence in patients with borderline personality disorder referred to an outpatient referral clinic in Tehran, Iran.Methods: The study was a retrospective cohort. The files of patients with borderline personality disorder referred to the outpatient clinic of the Tehran Psychiatric Institute were reviewed as the first step. In the next step, we contacted the patients and asked them to fill out the questionnaires. Data were collected using the Drug Attitude Inventory (DAI) questionnaire and a researcher made questionnaire to determine the attitude of patients toward pharmacological and non-pharmacological treatment and therapeutic adherence. After collecting data, patients’ therapeutic acceptance was divided into three groups: poor, partial, good compliance. The data were analyzed by SPSS software version-22.Results: Ninety-four patients were involved in the study and fifty four of them were women. In terms of psychotherapy adherence, patients with higher education and hospital admission history have better compliance. Medication attitudes were negative in 54 patients (57.4%), while 40.4% of them stated that psychotherapy or counselling did not help their condition and showed a negative attitude toward non-pharmacological treatment. Additionally, psychotherapy good adherence of the patients (44.7%) was higher than medication good adherence (31.9%). The most common reasons for discontinuation of treatment were medication side effects (53.1%), dissatisfaction with the therapist (40.3%) and then fear of medication dependence (40%). The results showed no relationship between other demographic factors and treatment adherence.Conclusions: Results of the current study show that attitude toward psychotherapy is more positive than pharmacotherapy. In addition, according to the results, working on changeable factors such as patients’ fear of dependence to medication, dissatisfaction with the therapist, and medication side effects may improve patients' treatment adherence.
Disrupting a socioecological system: how Traditional Ecological Knowledge could be the key to preserve Araucaria Forest in Brazil under climate change?
Authors: Tagliari Mario Muniz , Bogoni Juliano André , Blanco Graziela Dias , Cruz Aline Pereira , Peroni Nivaldo 
Published: March 2022

Abstract
Socioecological systems (SES) hinge on human groups and ecosystems, promoting interdependence and resilience to environmental disturbances. Climate change effects propagate from organism to biomes, likely influencing SES. In southern Brazil, Araucaria Forest is a typical SES due to the historical interaction between humans and biodiversity. We thus aimed to evaluate empirically and theoretically how climate change could disrupt this system by interviewing 97 smallholders and assessing their Traditional Ecological Knowledge (TEK). We evaluated and measured: (i) socioeconomic impact of araucaria’s nut-like seed (pinhão) trade; (ii) ethnoecological knowledge about climate change; and (iii) generated an ecosystem services network. We projected these empiric data with a projected loss of 50-70% of the Araucaria Forest due to climate change to quantify the risks of the potential disruption of this socioecological system. We found evidence that to avoid the disruption of the Araucaria Forests is paramount to value TEK holders, safeguard the historical socioecological interaction, and promote non-mutually exclusive measures in an integrative response to maintain the Araucaria Forests resilient to future disturbances.
Fourth thoracic vertebra as landmark for depth of right internal jugular vein catheterization in infants
Authors: Liu Guoliang , Zhang Jianmin , Wang Fang , Liu Heqi 
Published: March 2022

Abstract
Backgroundː The carina is considered a reliable marker for the depth of right internal jugular vein catheterization in infants on chest radiograph. In adult anatomy, the carina is typically located at the level of the fifth thoracic vertebra. We are not aware of a positional relationship between infant carina and thoracic vertebrae. Thus, we evaluated that a vertebral body may be at the same level as carina and can be as radiographic landmarks for the depth of right internal jugular vein catheterization in infants.Methodsː In this retrospective analysis, 108 infants (aged 1–12 months) who underwent congenital heart surgery between January 1, 2019 and June 30, 2019 were included. We analyzed the post-operative chest radiographs of those who underwent right internal jugular vein catheterization and assessed the positional relationship of the carina and vertebral bodies.Resultsː In total, 95 children were enrolled; The carina was located at the third thoracic vertebra in 2 cases (2%) and at the fourth thoracic vertebra in 93 cases (98%). There was a significant difference in the position of the carina (P=0.01, P<0.05; respectively).Conclusionsː Most fourth thoracic vertebrae were at the same level as the carina on chest radiographs. Therefore, it has potential as a radiographic landmark for the depth of right internal jugular vein catheterization in infants on chest radiograph.
Color Transparent Monitor using Si/SiO2 Core-Shell Nanoparticles: Optimum Color Selection
Authors: Seyyedi Maryam , Rostami Ali 
Published: March 2022

Abstract
In this paper, core-shell nanoparticles are used to provide a transparent high-quality display. To realize this idea, Si-SiO2 core-shell nanoparticles have been used to achieve the most suitable nanoparticle composition for a transparent color display with high transparency and contrast. For color transparent monitor the superimposed QDs are used. There is a basic question for the realization of the proposed transparent monitor. What is the ratio of nanoparticles (ratio of three QDs) for three colors in the proposed monitor? In this paper, we are going to acknowledge this question. As it is clear, the scattering peaks for red, green, and blue are different because of the physics of the scattering that is wavelength-dependent. Thus, the optimum ratio for each color should be obtained. The numerical method of Finite-Difference Time-Domain is used to simulate and calculate the optical properties of the proposed transparent monitor. The effect of different combinations of nanoparticles to have the maximum dispersion at blue, green, and red wavelengths and the minimum absorption at other wavelengths has been investigated. Finally, the optimum ratio and arrangement of nanoparticles for all colors are reported and numerically evaluated.
El Niño controls the evolution of shorelines worldwide
Authors: Almar Rafael , Graffin Marcan , Boucharel Julien , Abessolo Gregoire Ondoa , Thoumyre Gregoire , Papa Fabrice , Montano Jennifer , Bergsma Erwin , Baba Mohamed Wassim , Jin Fei-Fei , Ranasinghe Roshanka 
Published: March 2022

Abstract
Coastal zones are fragile and complex dynamical systems that are increasingly under threat from the combined effects of anthropogenic pressure and climate change. Yet, the key environmental factors that drive regional coastline changes remain poorly quantified. Here, using global satellite derived shoreline positions from 2000 to 2017 and a variety of reanalysis products, we demonstrate that coastlines are under the influence of 3 main drivers: the sea-level, and also ocean waves and fluvial inputs. The relative contribution of each of the drivers vary across the global coastline, with about a third exhibiting a clear dominance of one of the drivers (60% for sea level, 30% for rivers and 10% for waves). Furthermore, by establishing that these environmental forcing are all substantially constrained by El Niño Southern Oscillation (ENSO) at interannual time scales, we derive a conceptual global model of yearly shoreline changes that integrates the complex and diverse planetary climate influence of ENSO on each of these 3 drivers. This model reproduces well the observed shoreline changes with a global correlation of 0.4 and up to 0.6 in the tropical belt. We believe it represents a new solid physical and mathematical framework for understanding ENSO-driven littoral hazards as well as an efficient yet simple approach for their prediction.
Multi-Omics To Predict Changes During Cold Pressor Test
Authors: Kogelman Lisette J.A. , Ernst Madeleine , Falkenberg Katrine , Mazzoni Gianluca , Courraud Julie , Lundgren Li Peng , Laursen Susan Svane , Cohen Arieh , Olesen Jes , Hansen Thomas Folkmann 
Published: March 2022

Abstract
Background: The cold pressor test (CPT) is a widely used pain provocation test to investigate both pain tolerance and cardiovascular responses. We hypothesize, that performing multi-omic analyses during CPT gives the opportunity to home in on molecular mechanisms involved. Twenty-two females were phenotypically assessed before and after a CPT, and blood samples were taken. RNA-Sequencing, steroid profiling and untargeted metabolomics were performed. Each ‘omic level was analyzed separately at both single-feature and systems-level (principal component [PCA] and partial least squares [PLS] regression analysis) and all ‘omic levels were combined using an integrative multi-omics approach, all using the paired-sample design. Results: We showed that PCA was not able to discriminate time points, while PLS did significantly distinguish time points using metabolomics and/or transcriptomic data, but not using conventional physiological measures. Transcriptomic and metabolomic data revealed at feature-, systems- and integrative- level biologically relevant processes involved during CPT, e.g. lipid metabolism and stress response. Conclusion: Multi-omics strategies should be exploited in intervention studies, such as pain provocation tests, to gain knowledge on the biological mechanisms involved in complex traits.
ImageJ Assessment of Argon Laser Efficacy in Treatment of Mild Lower Eyelid Ectropion with Punctal Eversion
Authors: Salamah Moustafa , Enany Hesham , Hegab Mohammed Ahmed , bialy Hani AL 
Published: March 2022

Abstract
Purpose: To describe a new, simple and minimally invasive technique for correction of mild medial lower lid ectropion with punctal evrsion and measuring the efficacy of the technique by measuring the tear film meniscus pre and post argon laser treatment using imagej software.Methods: This study included 23 eyes, 19 patients with lower eyelid punctal eversion. All patients were treated by argon laser of lower medial conjunctiva. Tear film thickness was assessed using imagej software pre and post argon laser treatment.Results: There was a highly significant difference between before and after argon laser treatment as regarding the height of tear film which was found to be significantly lower after argon laser treatment compared to before it (81.1pixels versus 193.1pixels respectively) (p< 0.001). Conclusion: In conclusion, argon laser to lower medial palpebral conjunctiva is a simple, easy, safe, and effective procedure which can be applied as a sole treatment in early cases of punctal eversion with no or mild medial canthal tendon laxity.Clinical Trials Registration no clinical trial registration.
Microarray analysis of mRNA expression profiles in liver of ob/ob mice with real-time atmospheric PM2.5 exposure
Authors: Lin Lisen , tian li , Li tianyu , sun mengqi , duan junchao , Yu Yang , sun zhiwei 
Published: March 2022

Abstract
Epidemiological studies have demonstrated the association between exposure to fine particulate matter (PM2.5) and the onset of Non-alcoholic fatty liver disease (NAFLD). However, the potential biological mechanism is largely unknown. Our study was aimed to explore the impact of PM2.5 on the transcriptome level in the liver of ob/ob mice by atmosphere PM2.5 whole-body dynamic exposure system, and meanwhile preliminarily investigated the effects of metformin intervention in this process. 3,574 differentially expressed genes (DEGs) was screened out by microarray analysis (p<0.05, FC>1.5). KEGG pathway enrichment analysis showed that these DEGs were mainly enriched in cancers, infectious diseases and signal transduction, and the most significant pathway were thyroid hormone signaling pathway, chronic myeloid leukemia and metabolic pathways. Then, 12 hub genes were gained through weighted gene correlation network analysis (WGCNA) and verified by qRT-PCR. The expression of 5 genes in darkslateblue module (cd53, fcer1g, cd68, ctss, laptm5) increased after PM2.5 exposure, and decreased after metformin intervention. They were related to insulin resistance, glucose and lipid metabolism and other liver metabolism, and also neurodegenerative diseases. This study provided valuable clues and possible protective measures to the liver damage in ob/ob mice caused by PM2.5 exposure, and further research is needed to explore the related mechanism in detail.
Genetic dissection of behavioral traits related to successful training in drug-detection dogs
Authors: Matsumoto Yuki , Konno Akitsugu , Ishihara Genki , Inoue-Murayama Miho 
Published: March 2022

Abstract
Drug detection dogs play integral roles in society; however, the interplay between their behaviors and genetic characteristics remains uninvestigated. To profile the genetic traits associated with various behaviors related to the successful training of drug detection dogs, we collected and analyzed more than 230,000 genetic variants from 326 dogs belonging to the German Shepherd or Labrador Retriever breeds. Behavioral breed differences were observed in ‘friendliness to humans’ and ‘tolerance to dogs’. A strong positive correlation was observed in the genomic heritability of behavioral traits between breeds (Pearson’s r = 0.964, P = 0.008), indicating a similar degree of genetic influence on the behavioral traits between breeds. A genome-wide association study identified 18 single nucleotide polymorphisms potentially associated with drug detection abilities and three behavioral traits (interest in the dummy, tolerance to dogs, and friendliness to humans) related to drug detection abilities. Among them, 61 protein coding genes, including those associated with anxiety-related or exploration behavior in mice, such as Atat1 and Pfn2, were located surrounding the candidate polymorphisms. These findings highlight genetic characteristics associated with behavioral traits that are important for the successful training of drug detection dogs, which might support improved breeding and training of these dogs.
Highly Integrated Bionic Prostheses Resolve The Thermal Asymmetry Between Residual Amputated And Contralateral Limbs
Authors: Lang Victoria , Munoz-Novoa Maria , Ortiz-Catalan Max 
Published: March 2022

Abstract
Residual limbs after amputation present colder temperatures than unaffected contralateral limbs. This temperature asymmetry has been attributed to autonomic and cognitive factors, such as changes in body representation. An ideal limb replacement should restore the body representation and resolve the temperature asymmetry, but conventional prostheses, commonly characterized as disembodied, fail to do so. Neuromusculoskeletal prostheses are a new concept of artificial limbs that directly interface with the user’s nerves, muscles, and skeleton, and are operated in daily life by bidirectionally transferring control and somatosensory information. Here, we show that the temperature asymmetry is resolved in neuromusculoskeletal but not conventional prostheses. Increased ownership and agency inducing embodiment of the neuromusculoskeletal prostheses and could be a potential explanation of the phenomenon, suggesting a cognitive neural basis of the temperature asymmetry commonly found with amputation.