bluuebunny/crossref_metadata_2025_split huggingface dataset:
192Ir brachytherapy combined with external beam radiation and biliary stenting can improve the local control rate of unresectable hilar cholangiocarcinoma
Authors: Yang Wenbo , Li Yang , Xiao Li , Liu Fei , Sun Yunchuan 
Published: June 2022

Abstract
Objective
To evaluate the prognostic efficacy and safety of 192Ir brachytherapy combined with external beam radiation in the treatment of unresectable hilar cholangiocarcinoma.
Methods
The clinical data of patients with unresectable hilar cholangiocarcinoma admitted to Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine in Hebei Province from January 2014 to March 2019 were retrospectively analyzed. The treatment mode was concurrent chemoradiotherapy + biliary stent +/- brachytherapy. Survival was analyzed using the Kaplan–Meier method.
Results
This study included 62 patients; 32 received 192Ir brachytherapy combined with external beam radiation and percutaneous hepatobiliary stenting as the treatment group, and 30 received external beam radiation and percutaneous hepatobiliary stenting as the control group. After 3 months of treatment, the complete remission rates (CRs) of the treatment group and the control group were 15.6% and 6.7%, respectively, the partial remission rates (PRs) were 78.1% and 63.3%, and the objective remission rates (ORRs) were 93.7% and 70%, respectively. The median progression-free survival (PFS) of the treatment group and the control group was 11.0 months and 8.5 months, respectively. The 1-year PFS rates for the treatment group and the control group were 37.5% and 6.3%, respectively, and the 2-year PFS rates were 16.7% and 0%, respectively. There was a statistically significant difference in the progression-free survival rate between the two groups (P < 0.05). The median overall survival (OS) of the treatment group and the control group was 14.5 months and 12.2 months, respectively. The 1-year OS rates for the treatment group and control group were 58.8% and 27.9%, respectively; the 2-year OS rates were 51.6% and 14.7%, respectively. There was no significant difference in the overall survival rate between the two groups (P > 0.05). TBIL, DBIL, ALT, AST and CA19-9 in the treatment group were significantly lower than those before treatment and 3 months after treatment in the control group, and the difference was statistically significant (P < 0.05). No serious complications, such as bile leakage or biliary tract hemorrhage, occurred.
Conclusion
192Ir brachytherapy combined with external beam radiation and biliary stenting might improve the local control rate of unresectable hilar cholangiocarcinoma, with reliable prognostic efficacy and tolerable adverse reactions, providing a feasible and safe treatment method for clinical practice.
The impact of cell culture media on the interaction of biopolymer-functionalized gold nanoparticles with cells: mechanical and toxicological properties
Authors: Meesaragandla Brahmaiah , Komaragiri Yesaswini , Schlüter Rabea , Otto Oliver , Delcea Mihaela 
Published: June 2022

Abstract
Understanding the nanoparticle-cell interactions in physiological media is vital in determining the biological fate of the nanoparticles (NPs). These interactions depend on the physicochemical properties of the NPs and their colloidal behavior in cell culture media (CCM). Furthermore, the impact of the bioconjugates made by nanoparticle with proteins from CCM on the mechanical properties of cells upon interaction is unknown. Here, we analyzed the time dependent stability of gold nanoparticles (AuNPs) functionalized with citrate, dextran-10, dextrin and chitosan polymers in protein poor- and protein rich CCM. Further, we implemented the high-throughput technology real-time deformability cytometry (RT-DC) to investigate the impact of AuNP-bioconjugates on the cell mechanics of HL60 suspension cells. We found that dextrin-AuNPs form stable bioconjugates in both CCM and have a little impact on cell mechanics, ROS production and cell viability. In contrast, positively charged chitosan-AuNPs were observed to form spherical and non-spherical aggregated conjugates in both CCM and to induce increased cytotoxicity. Citrate- and dextran-10-AuNPs formed spherical and non-spherical aggregated conjugates in protein rich- and protein poor CCM and induced at short incubation times cell stiffening. We anticipate based on our results that dextrin-AuNPs can be used for therapeutic purposes as they show lower cytotoxicity and insignificant changes in cell physiology.
Development of mechanical strengthened, UV-shielding and antibacterial hydroxyethyl cellulose-based composite films
Authors: Zhang Xueqin , Guo Haoqi , Luo Wenhan , Chen Guojian , Xiao Naiyu , Xiao Gengsheng , Liu Chuanfu 
Published: June 2022

Abstract
Hydroxyethyl cellulose (HEC)-based semi-transparent, mechanical strengthened, UV-shielding and antibacterial ternary composite films were developed. Polyvinyl alcohol (PVA) and ε-polylysine (ε-PL) were respectively used as reinforcing agent and antibacterial agent, and covalent bonds among these three components were constructed with epichlorohydrin (ECH) to achieve chemical cross-linking. The mechanical properties of the composite films were largely enhanced by chemical cross-linking, and the maximum tensile strength and elongation at break were 95.9 ± 4.1 MPa and 148.8 ± 2.6%, respectively. FT-IR and XRD analyses indicated that chemical structure of the composite films could be well controlled by varying component proportion. From UV-Vis analysis, the optimum values of the percentage of blocking from UV-A and UV-B and UPF values were 98.35%, 99.99% and 60.25, respectively. Additionally, the composite films showed moderate water vapor permeability and swelling behavior. Moreover, the presence of ε-PL contributed to the good antibacterial activity of the composite films.
Post translational modifications in the complement system associated with idiopathic membranous nephropathy
Authors: Zhang Shaolei , Liu Dongwei , Zhang Yu , Tian Fei , Zhao Zihao , Chen Fengmei , Liu Zhangsuo , Yang Jing-Hua 
Published: June 2022

Abstract
Objective: To explore the molecular pathogenesis of idiopathic membranous nephropathy (IMN) at the level of post-translational modifications (PTMs) using the shotgun proteomics and a modified open search analysis. Methods: Serum proteins from 41 IMN patients and 92 healthy people were analyzed by high resolution mass spectrometry. The mass differences between the coding and observed amino acid residues crossing the proteomes, termed the amino acid variations or polymorphisms, were determined with the open search engine followed by multivariate clustering, Gaussian regression and amino acid localization. The biological functions and interaction networks of these amino acid variants were correlated with the pathogenesis of IMN using bioinformatics analysis. Results: A total of 2627 clustered delta masses were identified, spreading over 27597 sites on 457 proteins. Among them, the levels of amino acid variants at 159 proteins were upregulated in patients with IMN, and 20 downregulated. The proteins with dysregulated amino acid variants were predominantly related to LXR/RXR activation, acute phase response signaling, coagulation system as well as the complement system. Markedly, the complement factors such as C3, C4B, C4BPA, C8g and CFH were the top proteins with Leu/Ile>Val and Asn>Asp substitutions, binding with calcium ions, and unknown PTMs, such as C4BPA@372N>D, C4B@141L>V, C7@389S>T, and CFH@339X>T. The functional analysis of proteins showed that nephrotoxicity (Global injury, renal inflammation, renal nephritis, kidney failure, renal damage) was associated with kidney disease, involving 28 kinds of proteins, including C4A/C4B. Through IPA analysis, we found that C4A/C4B interacted with many molecules, such as extracellular, cytoplasmic and nuclear. Conclusion: Complement pathway plays an important role in the pathogenesis of membranous nephropathy. The post-translational modification of C4B may be the most important pathogenesis of membranous nephropathy in the complement pathway.
Phytoremediation of Methylene blue dye (triarylmethane) and Congo red (azo) by Trachyspermum ammi: Kinetic studies
Authors: Kaur Navjeet , Kaushal Jyotsna , Mahajan Pooja 
Published: June 2022

Abstract
Phytoremediation developed as a green-clean, solar-driven and innovative technique for the eradication of contaminants from the environment from last two decades. In the present research work, phytoremediation potential of an ornamental plant Trachyspermum ammi L. (T. ammi) was examined for the removal of a triarylmethane-Methylene blue (MB) and di-azo-Congo red (CR) dye. The various parameters such as initial dye concentration, contact time, and pH were focused to explore the dye removal capability of T. ammi. in wastewater. The increase in initial dye concentration affects the decolorization potential of the T. ammi. The decolorization potential of T. ammi found to be reduced with enhancing the initial dye concentration of both the dyes. The plant achieved condition of equilibrium after the 40 h of contact time with both MB and CR dyes. The kinetics of phytoremediation reaction with both MB and CR dyes were studied with the help of pseudo-first order, pseudo-second order, and Elovich models and the results were well appropriated to pseudo-first order with the correlation value R2 ≥ 0.99 for MB and R2 ≥ 0.97 for CR. The Langmuir and Freundlich isotherms studies of phytoremediation reaction found in favor of Freundlich equilibrium isotherm with correlation R2 ≥ 0.99 for both MB and CR dyes. The finding of analytical studies disclosed that the phytoremediation mechanisms of MB and CR by T. ammi firstly, involves phytoadsorption through electrostatic interaction and hydrogen bonding and then degradation of dyes occur through desulfurization and denitrification.
Dynamic alternations in mother-infant dyad’s gut microbiota over the period of six months
Authors: Yang Lan , Sakandar Hafiz Arbab , Sun Zhihong , Zhang Heping 
Published: June 2022

Abstract
Since birth, the human microbiome plays a pivotal role in health. It is intriguing to understand the establishing roles of intestinal microbiota in infants. This study aimed to investigate the dynamic changes in maternal and infant’s gut microbiota, the interaction between intestinal microbiota, and the function of intestinal microbiota. This study recruited one pregnant woman > 35 weeks gestational age. Faeces of the mother-infant dyad were regularly collected until six months of infant’s age for metagenomic analysis. The results showed the infant’s gut microbiota was different from the mother’s, majorly, encompassing Bacteroides fragilis, Ruminococcus gnavus, Klebsiella pneumoniae and Klebsiella michiganensis. Infant- or mother-specific differential metabolic pathways were found between the mother and infant’s gut microbiome, implicating differences in the intestinal metagenomic potential/function. In conclusion, the gut microbes and functions were gradually established as infant grew.
WITHDRAWN: Pembrolizumab combined with chemotherapy to treat parotid adenocarcinoma with pulmonary metastasis: a case report
Authors: Wu YaDi , Zhang HouYun , Wang He , Zhang LiFang , Liu YanTing , Zhou HongYan , Lu Ping , Zhang Min 
Published: June 2022

Abstract
Background: Our research for treating parotid adenocarcinoma was relatively slow due to its rare incidence. The consensus for treating pulmonary metastasis from parotid adenocarcinoma remains unclear as modern medicine is ineffective for patients with distant metastasis of primary parotid adenocarcinoma.Case presentation: A male patient with pulmonary metastasis from primary parotid adenocarcinoma was presented and received three courses of pembrolizumab combined with chemotherapy as a first-line treatment. After which, there was an opportunity for surgery. No tumor cells were found in postoperative pathology. The patient achieved a complete response (CR) that continued on a follow-up of more than one year.Conclusions: This case emphasizes a definitive multimodal approach, with immunotherapy being a promising treatment in patients with pulmonary metastasis from parotid adenocarcinoma.
Synthesis of a novel poly-carboxylic acids via click reaction and its application for easy-care treatment of cotton fabrics
Authors: Hu Hanchang , Li Fuqiang , Dong Xia , Zhao Qiangqiang , Wu Rongliang , Meng Chen , Xu Jiani , Cai Tingwei , He Jinxin 
Published: June 2022

Abstract
Maleic acids (MA) have been studied to replace formaldehyde-based crosslinking agents for anti-wrinkle treatment of cotton fabrics due to its low cost. However, the resilience of cotton fabrics treated by MA is not acceptable for human being. In this study, a novel poly-carboxylic acid of Ethyldimercaptan Disuccinic Acid (EDMDSA) was synthesized by MA and 1,2-Dimercaptoethane via click reaction and applied on anti-wrinkle finishing of cotton fabrics. The chemical structure of EDMDSA was confirmed by NMR and MS spectra. The results indicated that the recommended anti-wrinkle finishing condition were 0.3 mol. L-1 EDMDSA, 0.3 mol. L-1 SHP, curing temperature of 170°C and curing time of 90 s. After treating, the Wrinkle Recovery Angle of cotton fabrics finished by EDMDSA increased from 137.2 ° of untreated fabrics to 251.3 °, which was significantly higher than 216 ° of those treated by MA. Besides, due to more flexible structure of EDMDSA, the finished cotton fabrics have slightly weaker anti-wrinkle performance but higher strength retention than those treated by BTCA. Positively, facile synthesis and purification of EDMDSA as well as its excellent crosslinking performance indicates it has a potential industrial-scale application in the field of non-formaldehyde anti-wrinkle treatment.
Radiologic findings of immunocompromised and immunocompetent COVID-19 patients with semi-quantitative chest CT score system
Authors: Ghadery Abdolkarim Haji , Abbasian Ladan , Jafari Fatemeh , Yazdi Niloofar Ayoobi , Ahmadinejad Zahra 
Published: June 2022

Abstract
Background: As effects of immunosuppression are not still clear on COVID-19 patients, we conducted this study to identify clinical and laboratory findings associated with pulmonary involvement in both immunocompromised and immunocompetent patientsMethods: 107 immunocompromised and 107 immunocompetent COVID-19 patients matched for age and sex with either positive RT-PCR or clinical-radiological findings suggestive of COVID-19 enrolled in the study. Their initial clinical features, laboratory findings and chest CT scans, and short-term outcomes (hospitalization time and ICU admission) were recorded. In addition, pulmonary involvement was assessed with the semi-quantitative scoring system (0-25).Results: Pulmonary involvement was significantly lower in immunocompromised patients in contrast in immunocompetent patients especially in RLL (p-value=0.001), LUL (p-value=0.023), and both central and peripheral (p-value=0.002), and peribronchovascular (p-value=0.004) sites of lungs. Patchy (p-value<0.001), wedged (p-value=0.002), confluent (p-value=0.002) lesions with both ground glass and consolidation pattern (p-value<0.001) were significantly higher among immunocompetent patients.Initial sign and symptoms of immunocompromised patients including dyspnea (p-value=0.008) and hemoptysis (p-value=0.036*), respiratory rate of over 25 (p-value <0.001), and spo2 of below 93% (p-value= 0.01) was associated with higher pulmonary involvement. Total chest Ct score was also associated with longer hospitalization (p-value=0.016) and ICU admission (p-value=0.04) among immunocompromised patients.Conclusions: Immunocompromised patients had less severe pulmonary involvement, and initial clinical findings (dyspnea, hemoptysis, higher RR, and lower Spo2) could better predict pulmonary involvement than laboratory findings.
The Role of Meteorin-Like Peptide and TRPM2 in Differential Diagnosis in Benign, Premalignant and Malignant Lesions of the Endometrium
Authors: Balta Hilal , Kocaman Nevin 
Published: June 2022

Abstract
Endometrial cancer is the most common gynecological cancer in developed countries, and Type 1 endometrial adenocarcinoma develops from the background of atypical hyperplasia. Early diagnosis is effective on mortality and morbidity, and immunohistochemical biomarkers are the most helpful diagnostic method.The samples of this retrospective study were obtained from the pathology laboratory of the Fırat University, Faculty of Medicine, Department of Pathology. A total of 60 patient samples were used, with 10 samples in each group. The cases of proliferative endometrium, simple endometrial hyperplasia without atypia, simple atypical endometrial hyperplasia, complex endometrial hyperplasia without atypia, complex atypical endometrial hyperplasia, and endometrial endometrioid adenocarcinoma were included in the study. The tissue samples were treated and evaluated with Meteorin-like peptide (METRNL) and TRPM2 in all groups.Statistically significant relationships were detected between endometrial hyperplasia without complex atypia, endometrial hyperplasia with complex atypia, and Type-1 endometrial adenocarcinoma in terms of METRNL and TRPM2 immunoreactivity.The study results support the presence of a significant association in terms of the expression of METRNL and TRPM2 in endometrial tumors. The results of the present study confirm that both markers have diagnostic value for endometrioid carcinoma.
Biochemical and Microbiological Aspects of the Oral Cavity of Children and Young People With Neurological Impairment and Oropharyngeal Dysphagia
Authors: Duarte Janaina Costa Marangon , Costa Isabela Bazzo , Teixeira Daniel De Bortoli , Fregatto Luiz Fernando , Mendes Claudemir Gregorio , Mascarin Aline Maria Noli , Junior Salum Bueno Silveira , Serva Bianca Eduarda Bapstitella Mesquita , Comar Livia Picchi , Silva Roberta Gonçalves , Agostinho Francisco , Cola Paula Cristina 
Published: June 2022

Abstract
Objective
To compare the oral hygiene, biochemical, and microbiological aspects of the oral cavity of children and young people with neurological impairment and oropharyngeal dysphagia.
Method
Forty children and young people with neurological impairment and oropharyngeal dysphagia were enrolled and divided into two groups: group I, including 20 participants fed via gastrostomy; and group II, including 20 participants fed via the oral route. Oral hygiene and salivary pH and flow were assessed, and polymerase chain reaction was performed to evaluate the messenger RNA expressions of Porphyromonas gingivalis, Tanerella forsythia, and Treponema denticola.
Results
In groups I and II, the mean Oral Hygiene Index - Simplified scores were 4 and 2, respectively, showing a significant difference; the mean Calculus Index scores were 2 and 0, respectively, showing a significant difference; and the mean pH was 7.5 and 6.0, respectively, showing a significant difference. Bacterial analysis indicated no association between the two groups.
Conclusion
Children and young people who use gastrostomy had a poorer oral hygiene, greater dental calculus deposition, and higher salivary pH. Saliva of patients in both groups contained Porphyromonas gingivalis, Tanerella forsythia, and Treponema denticola.
Learning Curve of Microwave Ablation for Liver Cancers
Authors: Dou Jianping , Yu Jie , Cheng Wen , Wei Qiang , Luo Yanchun , Han Zhiyu , Cheng Zhigang , Liu Fangyi , Yu Xiaoling , Liang Ping 
Published: June 2022

Abstract
Background
Microwave ablation (MWA) has been increasing accepted as a curative therapy in small liver tumors but its learning curve is still unknown. This study aims to evaluate MWA efficacy and safety by cumulative MWA volume and interventional experience.
Materials
This study included 3113 primary liver cancers treated by MWA by 7 operators. Conditional logistic regression was used to estimate within-operators effects of increasing cumulative MWA volume per year on major complication, technical success (TS) and local tumor progression (LTP) rates were adjusted for treatment-level characteristics. Changes were also evaluated by subgroups of tumor size and tumor location.
Results
Lower severe complication rate was detected only in higher MWA volume (P = 0.02). Previous interventional experience had no influence on major complication rate. TE rates increased with the increase of MWA volume (all P < 0.00) per year. Similar results were found in subgroup analysis of interventional experience. LTP rates were similar regardless of MWA volume and interventional experience. MWA volume per year larger than 150 cases could reduce the major complication rate for tumors smaller than 5cm (P = 0.03) and tumors in higher risk location (P = 0.03). The increase of MWA volume per year could significantly increase the TS rate in all tumor size, expect for tumors in high-risk location.
Conclusion
Early and intensive performance of MWA procedures would reduce major complication rates regardless of tumor size and tumor location, but could not improve TS rate in high-risk locations.
Prevalence and risk factors of pelvic floor disorders after delivery in Japanese women using the PFDI-20: A single-center retrospective cohort survey
Authors: Suemitsu Tokumasa , Mikuni Kazumi , Matsui Hiroki , Suzuki Makoto , Takahashi Tomoko 
Published: June 2022

Abstract
Background: Female pelvic floor disorders (PFD) include various clinical conditions, such as urinary and fecal incontinence and pelvic organ prolapse. PFD assessment has been facilitated by developing disease-specific questionnaires, such as the Pelvic Floor Distress Inventory-20 (PFDI-20). We aimed to investigate the prevalence of PFD in Japanese women after delivery and the association of PFD with epidural anesthesia.Methods: This retrospective study included women who gave birth at our institution. The PFDI-20 questionnaire (validated in Japanese) was used to evaluate PFD symptoms 6–15 months postpartum. Results: Of 212 postpartum women, 156 (73.6%) manifested PFD symptoms. The most prevalent PFD symptom was urinary distress in 114 (53.8%) women, wherein 79 (37.3%) women experienced urine leakage related to increased abdominal pressure.Conclusion: A comparison of the epidural and non-epidural groups regarding the association between PFD and delivery mode revealed a higher disease burden score by 8.67 points in the epidural group. Therefore, correct diagnosis of women along with appropriate and regular follow-up until improvement of their symptoms would be significant. Healthcare workers should, therefore, inform pregnant women whether to choose vaginal delivery with or without anesthesia. To the best of our knowledge, our study is the first to investigate whole postpartum PFD in Japan. Trial registration: Retrospectively registered.