bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Viral Genotyping Reveals Prognostic Viral Phylogenetic Groups in HPV16-Associated Squamous Cell Carcinoma of the Oropharynx
Authors: Schrank Travis P. , Landess Lee , Stepp Wesley H. , Rehmani Hina , Weir William H. , Lenze Nicholas , Lal Asim , Wu Di , Kothari Aditi , Hackman Trevor G. , Sheth Siddharth , Patel Shetal , Jefferys Stuart R. , Issaeva Natalia , Yarbrough Wendell G. 
Published: June 2022

Abstract

Human papillomavirus–positive (HPV+) squamous cell carcinoma of the oropharynx (OPSCC) is the most prevalent HPV-associated malignancy in the United States and is primarily caused by HPV subtype 16 (HPV16). Favorable treatment outcomes have led to increasing interest in treatment deescalation to reduce treatment-related morbidity. Prognostic biomarkers are needed to identify appropriately low-risk patients for reduced treatment intensity. Targeted DNA sequencing including all HPV16 open reading frames was performed on tumors from 104 patients with HPV16+ OPSCC treated at a single center. Genotypes closely related to the HPV16-A1 reference were associated with increased numbers of somatic copy-number variants in the human genome and poor recurrence-free survival (RFS). Genotypes divergent from HPV16-A1 were associated with favorable RFS. These findings were independent of tobacco smoke exposure. Total RNA sequencing was performed on a second independent cohort of 89 HPV16+ OPSCC cases. HPV16 genotypes divergent from HPV16-A1 were again validated in this independent cohort, to be prognostic of improved RFS in patients with moderate (less than 30 pack-years) or low (no more than 10 pack-years) of tobacco smoke exposure. In summary, we show in two independent cohorts that viral sequence divergence from the HPV16-A1 reference is correlated with improved RFS in patients with moderate or low tobacco smoke exposure.

Implications:
HPV16 genotype is a potential biomarker that could be easily adopted to guide therapeutic decision-making related to deescalation therapy.
Activation of Tumor-Cell STING Primes NK-Cell Therapy
Authors: Knelson Erik H. , Ivanova Elena V. , Tarannum Mubin , Campisi Marco , Lizotte Patrick H. , Booker Matthew A. , Ozgenc Ismail , Noureddine Moataz , Meisenheimer Brittany , Chen Minyue , Piel Brandon , Spicer Nathaniel , Obua Bonje , Messier Cameron M. , Shannon Erin , Mahadevan Navin R. , Tani Tetsuo , Schol Pieter J. , Lee-Hassett Anna M. , Zlota Ari , Vo Ha V. , Ha Minh , Bertram Arrien A. , Han Saemi , Thai Tran C. , Gustafson Corinne E. , Venugopal Kartika , Haggerty Timothy J. , Albertson Thomas P. , Hartley Antja-Voy , Eser Pinar O. , Li Ze-Hua , Cañadas Israel , Vivero Marina , De Rienzo Assunta , Richards William G. , Abu-Yousif Adnan O. , Appleman Vicky A. , Gregory Richard C. , Parent Alexander , Lineberry Neil , Smith Eric L. , Jänne Pasi A. , Miret Juan J. , Tolstorukov Michael Y. , Romee Rizwan , Paweletz Cloud P. , Bueno Raphael , Barbie David A. 
Published: June 2022

Abstract

Activation of the stimulator of interferon genes (STING) pathway promotes antitumor immunity but STING agonists have yet to achieve clinical success. Increased understanding of the mechanism of action of STING agonists in human tumors is key to developing therapeutic combinations that activate effective innate antitumor immunity. Here, we report that malignant pleural mesothelioma cells robustly express STING and are responsive to STING agonist treatment ex vivo. Using dynamic single-cell RNA sequencing of explants treated with a STING agonist, we observed CXCR3 chemokine activation primarily in tumor cells and cancer-associated fibroblasts, as well as T-cell cytotoxicity. In contrast, primary natural killer (NK) cells resisted STING agonist–induced cytotoxicity. STING agonists enhanced migration and killing of NK cells and mesothelin-targeted chimeric antigen receptor (CAR)-NK cells, improving therapeutic activity in patient-derived organotypic tumor spheroids. These studies reveal the fundamental importance of using human tumor samples to assess innate and cellular immune therapies. By functionally profiling mesothelioma tumor explants with elevated STING expression in tumor cells, we uncovered distinct consequences of STING agonist treatment in humans that support testing combining STING agonists with NK and CAR-NK cell therapies.
Escuchando a los abuelos: transdisciplina, aves y gente para cultivar la memoria biocultural
Authors: Ibarra José Tomás , Caviedes Julián , Barreau Antonia , Pessa Natalia , Valenzuela Jeannette , Navarro-Manquelef Silvia , Pizarro J. Cristóbal 
Published: June 2022

(analítico)Presentamos una iniciativa y proponemos una metodología transdisciplinaria para cultivar la memoria biocultural, basada en los procesos de participación y materialización en comunidades de práctica (educativas). Presentamos el proyecto «Escuchando a los abuelos», que buscó facilitar diálogos intergeneracionales en tres escuelas mapuche (~ 90 niños y niñas) en Wallmapu, Chile. «Escuchando a los abuelos» utilizó a las aves como protagonistas de narrativas locales sobre el territorio. Cocreamos un ciclo de cinco pasos para promover la participación y la materialización. Los niños y niñas desarrollaron un ejercicio de abstracción para dar significado a las narrativas que ellos mismos recopilaron para crear memes positivos sobre las aves. Estos memes fueron comunicados dentro y más allá de sus comunidades. Concluimos que la experiencia de los abuelos debe ser honrada para contrarrestar la actual extinción de la experiencia biocultural.
Palabras clave: Arte-ciencia; comunidades de práctica; educación ambiental; etnoornitología; extinción de la experiencia; mapuche; socio-ornitología.
Endothelial Cell Insulin Signaling Regulates CXCR4 (C-X-C Motif Chemokine Receptor 4) and Limits Leukocyte Adhesion to Endothelium
Authors: Rathjen Thomas , Kunkemoeller Britta , Cederquist Carly T. , Wang Xuanchun , Lockhart Sam M. , Patti James C. , Willenbrock Hanni , Olsen Grith Skytte , Povlsen Gro Klitgaard , Beck Hans Christian , Rasmussen Lars Melholt , Li Qian , Park Kyoungmin , King George L. , Rask-Madsen Christian 
Published: June 2022

Background:
An activated, proinflammatory endothelium is a key feature in the development of complications of obesity and type 2 diabetes and can be caused by insulin resistance in endothelial cells.

Methods:
We analyzed primary human endothelial cells by RNA sequencing to discover novel insulin-regulated genes and used endothelial cell culture and animal models to characterize signaling through CXCR4 (C-X-C motif chemokine receptor 4) in endothelial cells.

Results:
CXCR4 was one of the genes most potently regulated by insulin, and this was mediated by PI3K (phosphatidylinositol 3-kinase), likely through FoxO1, which bound to the CXCR4 promoter. CXCR4 mRNA in CD31+ cells was 77% higher in mice with diet-induced obesity compared with lean controls and 37% higher in db/db mice than db/+ controls, consistent with upregulation of CXCR4 in endothelial cell insulin resistance. SDF-1 (stromal cell–derived factor-1)—the ligand for CXCR4—increased leukocyte adhesion to cultured endothelial cells. This effect was lost after deletion of CXCR4 by gene editing while 80% of the increase was prevented by treatment of endothelial cells with insulin. In vivo microscopy of mesenteric venules showed an increase in leukocyte rolling after intravenous injection of SDF-1, but most of this response was prevented in transgenic mice with endothelial overexpression of IRS-1 (insulin receptor substrate-1).

Conclusions:
Endothelial cell insulin signaling limits leukocyte/endothelial cell interaction induced by SDF-1 through downregulation of CXCR4. Improving insulin signaling in endothelial cells or inhibiting endothelial CXCR4 may reduce immune cell recruitment to the vascular wall or tissue parenchyma in insulin resistance and thereby help prevent several vascular complications.
Relationship of Sodium Intake With Granulocytes, Renal and Cardiovascular Outcomes in the Prospective EPIC‐Norfolk Cohort
Authors: Wenstedt Eliane F. E. , Peters Sengers Hessel , Boekholdt S. Matthijs , Khaw Kay‐Tee , Wareham Nicholas J. , van den Born Bert‐Jan H. , Vogt Liffert 
Published: June 2022

Background
Experimental studies show that high‐sodium intake affects the innate immune system, among others with increased circulating granulocytes. Whether this relationship exists on a population level and whether this relates to disease outcomes is unclear. We aimed to test the hypotheses that (1) sodium intake is associated with granulocytes on a population level; (2) granulocytes are associated with the presence of hypertension and both cardiovascular and renal outcomes; and (3) the relation between high‐sodium intake and these outcomes is mediated by granulocytes.

Methods and Results

We performed an analysis in 13 804 participants from the prospective EPIC (European Prospective Investigation into Cancer)‐Norfolk cohort, with a mean age of 58 years and median follow‐up of 19.3 years. Analyses were carried out using calculated estimated sodium intake and sodium‐to‐potassium ratios from spot urines at baseline. The main outcomes were hypertension at baseline, and composite cardiovascular (mortality or cardiovascular events) and renal (mortality or renal events) outcomes during follow‐up. Sodium intake and urine sodium‐to‐potassium ratio were positively associated with circulating granulocyte concentrations after adjustment for confounders (β=0.03;
P
=0.028 and β=0.06;
P
<0.001, respectively). Granulocytes significantly mediated the associations of, respectively, sodium intake and urine sodium‐to‐potassium ratio with hypertension at baseline, and cardiovascular and renal outcomes.

Conclusions
Sodium intake is positively associated with circulating granulocyte concentrations, and higher granulocyte concentrations associate with worse long‐term cardiovascular and renal outcomes. Given the recently established immune‐modulating effects of sodium and the role of immune cells in both cardiovascular and renal disease, causality for this pathway may need consideration in further studies.
Carotid Stenting Versus Endarterectomy for Asymptomatic Carotid Artery Stenosis: A Systematic Review and Meta-Analysis
Authors: Wang Jie , Bai Xuesong , Wang Tao , Dmytriw Adam A. , Patel Aman B. , Jiao Liqun 
Published: June 2022

Background:
To compare safety and efficacy between carotid artery stenting (CAS) and carotid endarterectomy (CEA) in treating asymptomatic carotid artery stenosis based on results from randomized controlled trials.

Methods:
Randomized controlled trials comparing CAS and CEA in treating asymptomatic carotid artery stenosis were searched from databases of the EMBASE, PubMed, MEDLINE, and Cochrane libraries. Two independent reviewers identified eligible studies, extracted relevant data, and used the Cochrane risk of bias tool to assess quality. Mantel-Haenszel method random-effects models were used to estimate odds ratio (OR) regarding perioperative risks between CAS and CEA. Kaplan-Meier curve data were extracted and analyzed through Exp[(O-E)/Var] fixed-effect models to calculate the Peto odds ratio (OR) regarding long-term outcomes.

Results:

Sixteen articles from 7 randomized controlled trials were included, reporting relevant outcomes for 7118 asymptomatic carotid artery stenosis patients (CAS: n=3920; CEA: n=3198). Compared with the CEA group, CAS group had no difference in perioperative composite end point events including stroke, death, and myocardial infarction (MI; OR, 1.13 [95% CI, 0.87–1.47];
P
=0.37, I
2
=0%). Compared with CEA, CAS had a higher risk of any stroke during the perioperative period (OR, 1.62 [95% CI, 1.16–2.24];
P
=0.004, I
2
=0%) and an increased risk of nondisabling stroke (OR, 1.81 [95% CI, 1.23–2.65];
P
=0.003, I
2
=0%), but there was no significant difference in disabling stroke and death between groups (OR, 0.91 [95% CI, 0.50–1.65];
P
=0.76, I
2
=0%). For long-term outcomes, no difference regarding the composite outcome of any stroke, death, and myocardial infarction existed between CEA and CAS (Peto OR, 1.18 [95% CI, 0.94–1.48];
P
=0.14, I
2
=0%). Individual-level patient data would be important to verify the long-term outcome results.

Conclusions:
When treating asymptomatic carotid artery stenosis, CAS has comparable perioperative and long-term composite outcomes compared with CEA. However, CAS may have a higher risk of any stroke and nondisabling stroke in the perioperative period.
Investigation of Microphysical Properties within Regions of Enhanced Dual-Frequency Ratio during the IMPACTS Field Campaign
Authors: Finlon Joseph A. , McMurdie Lynn A. , Chase Randy J. 
Published: June 2022

Abstract
Multifrequency airborne radars have become instrumental in evaluating the performance of satellite retrievals and furthering our understanding of ice microphysical properties. The dual-frequency ratio (DFR) is influenced by the size, density, and shape of ice particles, with higher values associated with the presence of larger ice particles that may have implications regarding snowfall at the surface. The Investigation of Microphysics and Precipitation for Atlantic Coast-Threatening Snowstorms (IMPACTS) field campaign involves the coordination of remote sensing measurements above winter midlatitude cyclones from an ER-2 aircraft to document the fine-scale precipitation structure spanning four radar (X-, Ku-, Ka-, and W-band) frequencies and in situ microphysical measurements from a P-3 aircraft that provide additional insight into the particle size distribution (PSD) behavior and habits of the hydrometeors related to the DFR. A novel approach to identify regions of prominently higher Ku- and Ka-band DFR at the P-3 location for five coordinated flights is presented. The solid-phase mass-weighted mean diameter (Dm) was 58% larger, the effective density (ρe) 37% smaller, and the liquid-equivalent normalized intercept parameter (Nw) 74% lower in regions of prominently higher DFR. Microphysical properties within a triple-frequency framework suggest signatures consistent with aggregation and riming as in previous studies. Last, a pretrained neural network radar retrieval is used to investigate the vertical structure of microphysical properties associated with the larger DFR signatures and provides the spatial context for inferring certain microphysical processes.

Significance Statement
The purpose of this study is to better understand what radar measurements from multiple frequencies can tell us about the sizes, shapes, and concentrations of ice particles within winter snowstorms, and how these observations are related to banded precipitation structures since they can have implications for snowfall at the surface. Our results show that ice particles are on average larger and less dense when the reflectivity difference between two radars operating at different wavelengths is larger and supports the process by which crystals aggregate to form larger particles. These findings aim to improve how satellites and forecasting models represent precipitation in the cloud and at the surface.
Can Customized YouTube Videos Improve ABSITE Performance?
Authors: Hanson Nicole , Diaz Graal , Romero Javier , Steen Shawn 
Published: June 2022

Introduction The COVID-19 pandemic decreased in-person didactic sessions in our general surgery residency. We piloted a program to develop online tutorials posted to a YouTube channel designed to prepare our residents for the ABSITE examination. Methods Study participants were General Surgery residents in our five-year ACGME accredited general surgery residency program who took the ABSITE in-service exam between 2019 and 2021. We compared raw scores and overall programmatic percentile scores for three academic years (2018-2019, 2019-2020, and 2020-2021). Results From academic year 2018-2019 to 2019-2020, before introducing the YouTube teaching platform, average raw scores and percentile scores among our general surgery residents remained unchanged (raw scores 64 - 65% ( p = ns), percentile scores 39 to 37% ( p = ns)). However, raw ABSITE scores increased significantly after introducing the YouTube teaching platform. A one-way ANOVA showed a statistically significant difference in raw ABSITE scores for AY 2020-2021 compared to the two prior years (F(2, 30) = [1.193], P < 0.01). Mean percentile ranking scores (program-wide) increased 15% from AY 2018-2019 to AY 2020-2021 (Figure1). Conclusion Teaching via an online platform such as YouTube allows residents to review material at their own pace and schedule. It is other our hypothesis is that the online YouTube material presented in the Ventura Surgery School channel contributed, in part, to this relative improvement compared to resident peers in other institutions. Teaching via an online platform could be a valuable tool to educate surgical learners in our ever-changing teaching environment.
Physics-based modeling and multi-objective parameter optimization of excitation coil for giant magnetostrictive actuator used on fuel injector
Authors: Rong Ce , He Zhongbo , Xue Guangming , Zhou Jingtao , Zhao Zhenglong 
Published: June 2022

Serving as one of the core component, the excitation coil can exert remarkable influence on the output performance of giant magnetostrictive actuator (GMA) for electronic controlled fuel injector. In this paper, a multi-objective coil optimization scheme is proposed to balance the conflicting response speed and magnetic field intensity determined by the coil parameters. Firstly, a physics-based coil model is established for optimization, whose parameters can be directly calculated by the coil dimensions. Then, with the current response of the coil calculated, a multi-objective optimization framework is conducted attempting to get the selection guideline for the enameled wire diameter of the coil. The optimal choices exist when the outer diameter of the enameled wire falls in 0.9∼1.6 mm, and the inner/outer diameter ratio is relatively high. Finally, a series of experiments are conducted and the results indicate that the proposed model is able to accurately describe the current response throughout the operating frequencies, and the optimization scheme can provide a valuable roadmap to design coil for high performance GMA.
Integrating Qualitative Inquiry and Critical Whiteness in Psychology Research Methods Courses
Authors: Jordan Lorien S. 
Published: June 2022

Introduction This paper merges two neglected components within the psychological sciences broadly and research methods courses specifically: Critical whiteness and qualitative methodologies. Statement of the Problem In psychology programs, regardless of discipline, research courses remain one area where issues of race and racism, such as critical whiteness, are deemphasized. Similarly, methods courses rarely integrate qualitative inquiry and critical theory. Literature Review First, I briefly review the relevant literature on the state of qualitative research in psychology. I then discuss critical whiteness, contextualizing the idea of whiteness, before moving into a review of the current research on whiteness in psychology. Teaching Implications I present three experiential learning activities that further students’ skill development in qualitative methods while learning about three specific aspects of whiteness. Practicing observations, photovoice, and qualitative coding, students can reflect on the pervasiveness of white culture, colorblind racism, and racial microaggressions. Conclusion The activities described in this article provide instructors one avenue to engage various aspects of whiteness and qualitative methods, phenomena routinely overlooked in graduate training.
Thyroglossal duct cyst with hoarseness as the sole symptom and an intralaryngeal extension masquerading as a laryngeal mass: Clinical experience and literature review
Authors: Bao Yang-Yang , Ge Yan-ping , Zhang Xiong-Jian , Guo Yu , Dai Li-Bo , Zhou Shui-Hong , Lin Wei 
Published: June 2022

A thyroglossal duct cyst is the most common congenital disease in the neck. There are two age groups usually associated with thyroglossal duct cysts: 1–11 years in children and 30–60 years in adults. These midline neck masses are typically located anteriorly in the neck, inferior to the hyoid bone. We report an extremely rare case of an intralaryngeal thyroglossal duct cyst without a neck mass, presenting with hoarseness as the sole symptom. A 64-year-old man presented with a 3-month history of hoarseness. On physical examination, no neck mass or swelling was observed during cervical palpation. Laryngostroboscopy revealed a large submucosal mass in the right glottis and supraglottis, and mobility of the right vocal cord was restricted. Surgery was performed via an external approach to completely resect the cyst, together with the middle part of the hyoid bone. Histopathologic examination of the cyst led to a diagnosis of thyroglossal duct cyst. The patient recovered well and his voice returned to normal. Attention should be paid to the occurrence of rare types of thyroglossal duct cyst in unusual clinical sites. Adequate radiological examinations should be performed, and reading the computed tomography or magnetic resonance imaging scans carefully before surgery is important to avoid misdiagnosis.
Shifting Authority: Indigenous Law-Making and State Governance
Authors: Sarson Leah 
Published: June 2022

How can Indigenous law-making affect state authority? I examine this in the context of Canada’s extractive sector, where I question how and when Indigenous laws prevail over state laws to challenge colonial authority and reassert Indigenous self-determination. Although the state claims supreme authority, Indigenous governments are enacting their own laws, laws that are sometimes in tension with those of the state. I demonstrate that while the state usually responds to contentious Indigenous law-making with conflict, under certain conditions Indigenous communities can incite the state to reverse its position to one of acquiescence or cooperation. By drawing on insights from three Indigenous communities in Canada, I offer four conditions under which Indigenous peoples and governments may be able to compel the state to relent and accrue authority, including by preparing to engage in a long-term project, by fomenting community cohesion, by exploiting evolving international norms related to Indigenous rights, and by offering viable governance alternatives to colonial legislation. By highlighting new sites of authority and resistance, this work underscores the transformative possibilities of Indigenous politics. Une autorité en évolution : pouvoir législatif autochtone et gouvernance publique