bluuebunny/crossref_metadata_2025_split huggingface dataset:
MO382: Validation of a Handheld Point-Of-Care Creatinine/EGFR Meter for Evaluating Renal Function
Authors: Begos Dennis , Milojkovic Bogdan 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Measurement of glomerular filtration rate (GFR) is a cumbersome, time consuming, and expensive process, thus estimated GFR (eGFR) has become a commonly accepted surrogate or screening tool for individuals suspected to have kidney disease. Until recently this required testing through phlebotomy and a large laboratory analyzer. Nova Biomedical (Waltham, MA, USA) has developed a point-of-care (POC) handheld device, the NovaMax Cr/eGFR System that is able to measure creatinine (Cr) and calculate an eGFR using a fingerstick (1.2 µL) of whole blood.

METHOD
A group of 517 subjects (48% male) were recruited for study at three different Clinical Research Organizations (CRO's). The patient population was from both healthy cohorts and those known to have CKD. Creatinine and eGFR was tested on all subjects using a finger stick sample with the NovaMax System and compared with venous blood on a Siemens RXL (Siemens Medical Solutions, Malvern, PA, USA). The CKD-EPI formula was used to calculate eGFR.

RESULTS
The average age was 61.1 years (range 18–91). The range of Cr in the groups were as follows: NovaMax 0.57—&gt;7 mg/dL (7 is the maximum reading), and for the Siemens 0.36–16.79 mg/dL. The range of eGFR was &lt;7–127 mL/min/1.73 m2 on the NovaMax, and 4.3–137 mL/min/1.73 m2 on the Siemens.
See Table 1 for sensitivity and specificity results.

CONCLUSION
The NovaMax Cr/eGFR System is an acceptable alternative for eGFR measurement in patients with a wide range of renal function. Its ability to measure renal function in a variety of settings make it a very useful device for several patient populations: screening patients at risk for CKD or AKI, telenephrology, population screening in low- and middle-income countries, following patients post kidney transplant, and evaluating patients prior to and after contrast imaging studies, among other indications.
MO394: Health Related Quality of Life in Patients With End-Stage Renal Disease Before and After Starting Dialysis During Pandemic Covid-19
Authors: Pana Camelia , Tuta Liliana-Ana 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Patients with end-stage renal disease (ESRD) have complex functional and social changes that affect their quality of life (QoL), leading to an increased cardiovascular risk, mortality and overall morbidity. Several studies showed that general QoL worsened after dialysis initiation but improved by 12 weeks. The influence of coronavirus (COVID-19) among pre-dialysis CKD patients starting dialysis during pandemic COVID-19 is challenging and still largely unexplored.
To evaluate the quality of life in patients with ESRD before and after initiating dialysis during pandemic COVID-19, in Constanta County, a south-eastern Romanian region.

METHOD
We assessed 59 patients (mean age = 61.65year ± 11.36, Male : Female = 3:1, 13.79% employee) with ESRD before and after dialysis started (T1–12 weeks and T2–24 weeks), using KDQOL–36™, in the interval of time of 1 year (May 2020–May 2021).

RESULTS
QoL was severely affected in patients with diabetes and hypertension, especially because of diet restrictions (58.6%, P &lt; 0.01), fatigue (67.5%, P &lt; 0.01), concerns about the kidney disease (72.4%, P &lt; 0.05) and permanent need for medical services (48.6%, P = NS).
Most of the patients required acute initiation of haemodialysis on CVC (66.1%) due to uraemic manifestations and late referral, 34.4% started planned haemodialysis on AV fistula and 8.47% started planned peritoneal dialysis. An overall improvement of QoL was seen both in patients with urgent and planned dialysis initiation, in women group less than in males (P &lt; 0.05). Among dialysis patients at T2, we have found significantly lower levels for mean Mental Component Summary (MCS), calculated as 45.9 ± 14.1, about 3.1 points less than the majority of other studies. The following mental health-related symptoms were claimed during the COVID-19 pandemic ‘second and third waves’: feeling sad, worrying, feeling nervous and trouble falling asleep, even though dialysis patients seemed less impacted by social distancing measures.

CONCLUSION
The initiation of dialysis was an important factor in improving the QoL in our ESRD patients and the type of dialysis and planned initiation contribute substantially to the patient's perception of the disease and influence the main psycho-social parameters of QoL. COVID-19 pandemic affected the QoL of patients that started dialysis in the established interval of time, especially the mean MCS score, probably associated with the high morbidity and mortality of Romanian ESRD and dialysis patients.
MO397: Association of Serum NT-PRO-BNP Level With Cardiometabolic Risk Factors in Different Stages of Chronic Kidney Disease Patients
Authors: Alam Ripa Roksana , Chowdhury Maa , Shahnoor Alam Kazi , Alam Babrul , Talukder Ashaduzzaman , Chaudhury Sr , Iqbal Masud 
Published: May 2022

Abstract

BACKGROUND AND AIMS
The role of cardiac biomarker, NT-pro-BNP and its rising over time may identify chronic kidney disease (CKD) patients at increased risk of cardiovascular morbidity and mortality. Additionally, this may be correlated with altered cardio-metabolic risk factors predicting adverse outcomes. In this study, serum NT-pro-BNP level at different stages of CKD patients was measured to observe its association with different cardiac and metabolic risk factors

METHOD
This was a cross-sectional study carried out on stable CKD patients, stages 1–5, attending the outpatient department of a tertiary renal care facility. Serum creatinine, estimated glomerular filtration rate (eGFR) and urinary ACR were measured as renal function tests. While cardio-metabolic risk factors included blood pressure, body mass index (BMI), lipid profile, serum albumin, uric acid and hsCRP. Statistical analyses of the results were obtained by using window-based computer software devised with Statistical Packages for Social Sciences (SPSS-22).

RESULTS
Patients of CKD 5 (30%), stage 4 (23%), stage 3 (26%), stage 2 (18%) and in stage 1 (6.7%) included 149 subjects. In stages 1–5 respectively, they were matched for age 48 ± 12, 48 ± 12, 51 ± 14, 50 ± 12, and 50 ± 11 years, (P = NS); BMI 24 ± 0.9 24 ± 1, 24 ± 1, 24 ± 1 and 24 ± 1 kg/m2, (P = NS) and serum creatinine was variable as 1.5 ± 1.5, 1.7 ± 0.7, 2.6 ± 2.0, 3.0 ± 1.6 and 5.7 ± 2.7mg% (P &lt; 0.001). The mean NT-proBNP had a rising pattern in stages 1–5 showing 335 ± 674, 790 ± 1717, 1221 ± 1994, 2875 ± 6139 and 15645 ± 14741 pg/mL, (P &lt; 0.001). There were positive correlations of NT-pro-BNP with serum creatinine (P &lt; 0.001); CKD stages (P &lt; 0.001). TG (P &lt; 0.003); phosphate (P &lt; 0.008) and negative correlation with serum albumin (P &lt; 0.001).

CONCLUSION
Higher levels of NT-pro-BNP are seen in advancing stages of CKD. The positive association of NT-pro-BNP with lipids, phosphate and negative relation with albumin indicates its possible prognostic value for cardio-metabolic risk assessment.
MO404: Autophagy and Fibrotic Response is Genetically Determined in Mouse Mesangial Cells
Authors: Manzeger Anna , Mikone Krisztina , Mozes Miklos , Kokeny Gabor 
Published: May 2022

Abstract

BACKGROUND AND AIMS
C57Bl6/J (B6) mouse strain is resistant to several experimental models of renal fibrosis. Mesangial cells play an important role in the pathogenesis of glomerulosclerosis. Also, autophagy dysregulation was recently related to fibrosis. However, it is unclear whether autophagy dysregulation could be genetically determined in mice. Thus, we investigated autophagy and fibrotic response in primary mesangial cells isolated from fibrosis resistant (B6) and fibrosis prone (CBA) inbred mouse strains.

METHOD
Primary mesangial cells were isolated from 6-weeks old B6 and CBA male mice via magnetic bead separation technique. Glomerular outgrowths were maintained in RPMI medium for 21 days when cells were subcultured and characterized for mesangial markers. Mesangial cells with passage numbers P5 to P8 were seeded onto 24-well plates. Gene expressions were analyzed after 48-h of PBS (CTL) or TGF-β (10 ng/mL) treatment (n = 6/group). Data (mean ± SD) were analyzed using Kruskal–Wallis test.

RESULTS
Tgfb1 and Col1a1 expressions increased by 2.5-fold and 8-fold in TGF-β treated CBA cells as compared to B6 (P &lt; 0.01), respectively. The expression of Egr1 (early growth response factor-1) that participates in both fibrosis and autophagy increased by 2-fold in TGF-β treated CBA cells but remained unchanged in B6 cells (P &lt; 0.05). Similarly, the expression of core autophagy molecule Lc3b did not change in TGF-β treated B6 cells, but increased by 60% in CBA cells (P &lt; 0.05), accompanied by 1.5-fold increase of Sqstm1/p62. On the other hand, the expression of Mtmr14 that negatively regulates autophagy decreased by 30% in TGF-β treated CBA cells but remained unchanged in B6.

CONCLUSION
We conclude that genetic background of mice highly influences the TGF-β induced fibrotic and autophagy response of mesangial cells. Our results corroborate previous reports on autophagy dysregulation associated to fibrosis.
FUNDING: Hungarian Academy of Sciences BO/00 304/20/5, ÚNKP-21–5-SE-1, NTP-NFTÖ-21-B-0045
MO418: The Risk of Renal Co-Morbidities in Celiac Disease Patients Depends on the Phenotype of Celiac Disease
Authors: Nurmi Rakel , Pasternack Camilla , Salmi Teea , Hervonen Kaisa , Koskinen Inka , Järvelin Jutta , Huhtala Heini , Collin Pekka , Mustonen Jukka , Kaukinen Katri , Mäkelä Satu 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Albeit the association between renal disorders and celiac disease is still somewhat contradictory, an elevated risk of kidney diseases in patients with celiac disease has been shown [1, 2, 3]. Only case reports have described the link between glomerulonephritis and dermatitis herpetiformis, a cutaneous manifestation of celiac disease [4]. This study aimed to evaluate whether patients with various phenotypes of celiac disease have an increased risk of renal co-morbidities compared with matched references.
METHODS: The diagnoses of glomerulonephritis, diabetic nephropathy, interstitial nephritis and end-stage renal disease were collected from the Finnish National Hospital Discharge Register from 1970 to 2015 among 1440 celiac disease patients and 4296 age- and sex-matched references. Further, patients were divided into two cohorts, dermatitis herpetiformis and any other phenotype of celiac disease. We used Cox proportional-hazards model to compare the risk of renal co-morbidities between patients and reference individuals.

RESULTS
Celiac disease was positively associated with renal disorders even after adjusting for type 1 diabetes [hazard ratio (HR) 1.81, 95% confidence interval (CI) 1.18–2.76]. A 3-fold and 12-fold risk increase were seen for glomerulonephritis and IgA nephropathy, respectively. A similar risk increase for renal co-morbidities in dermatitis herpetiformis was not shown.
CONCLUSIONS: Celiac disease was associated with an increased risk of renal co-morbidities, especially glomerulonephritis. The risk depends on the phenotype of celiac disease. The possible association between renal disorders and celiac disease should be investigated more in the future and kept in mind also in clinical practice.
MO423: Does Renal Dysfunction Improve the Predictive Value of Hatch Score for Postoperative Atrial Fibrillation?
Authors: Gjerakaroska Radovikj Marija , Severova- Andreevska Galina , Jovev Sashko 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Postoperative atrial fibrillation (POAF) is a frequent arrhythmic complication in cardiac surgery. Studies of this potential complication have not been able to elucidate its aetiology nor to identify the predictors of its occurrence. The aim of this study was to evaluate the predictive role of a renal dysfunction for POAF in patients undergoing cardiac surgery interventions. We also aimed to determine the role of renal dysfunction in improving the diagnostic accuracy of hypertension, age, transitory attack/stroke, chronic obstructive pulmonary disease, heart failure (HATCH) score for POAF.

METHOD
This prospective monocentric observational study included 178 consecutive patients who underwent cardiac surgery interventions in the State University Clinic for Cardiac Surgery of Skopje, Republic of North Macedonia, between September 2017 and September 2018. Patients were divided into two groups, according to the absence or occurrence of POAF. HATCH score was calculated and recorded for each patient. A new R-HATCH score was derived by the addition of renal dysfunction to the existing HATCH score and its predictive value for POAF was tested and compared to HATCH.

RESULTS
POAF occurred in 90/178 patients (50.56%). The average estimated glomerular filtration rate (eGFR) in the group developing POAF was significantly lower, 78.4 ± 26.9 versus 93.0 ± 29.0 mL/min/1,73m2 (P = 0.0005). Patients with GFR &lt; 60mL/min/1.73m2 had almost a 5 times greater risk of developing POAF. The addition of renal dysfunction to HATCH score improved its predictive accuracy in our study. The area under the receiver operating characteristic curve increased from 0.73 to 0.76 (P &lt; 0.001).

CONCLUSION
The addition of preoperative renal dysfunction could improve the diagnostic accuracy of HATCH score for POAF.
MO426: Elucidation of Mechanism of Low-Birth-Weight-Related Fsgs in Rats by Rna-Seq Analysis of Cultured Podocytes From Low-Birth-Weight Rats
Authors: Imasawa Toshiyuki , Hirano Daishi , Unoki-Kubota Hiroyuki 
Published: May 2022

Abstract

BACKGROUND AND AIMS
The pathogenesis of low-birth-weight (LBW)-related nephropathy has been considered to be the result of intra-glomerular hyperfiltration during growth due to the presence of low nephron numbers. On the other hand, we hypothesized that there may be pathogenic factor(s) other than low nephron number in LBW individuals [1]. We reported the results of proteomic analysis of the renal cortex of LBW rats to show that metabolic abnormalities should exist in the kidneys of LBW individuals [2]. However, our result of the previous study was obtained by analysis using the renal cortex, which did not allow us to interpret the mechanism of focal segmental glomerulosclerosis (FSGS) formation, the most typical pathological picture of LBW-related nephropathy.

METHOD
A total of 5 LBW rats (birth weight 4.38±0.08) were obtained by 55% caloric restriction to pregnant maternal rats. The normal-birth-weight (NBW) rats (birth weight 6.48±0.18) were obtained by feeding normal diets to pregnant maternal rats. Kidneys were removed at 4 weeks of age, and after isolation of the glomeruli by sieving of kidneys, primary culture of glomerular epithelial cells (podocytes) was performed. Then, mRNAs were extracted from the cultured podocytes after 7 days and RNA-seq analysis was performed using these mRNAs from LBW podocytes and NBW podocytes.

RESULTS
Gene expression of Nphs1 (nephrin), Nphs2 (podocin) Podxl (podocalyxin) was detected in all samples of both groups, confirming that podocytes were cultured. The number of transcripts detected was 17 967 genes. In addition, by comparison of mRNA expression levels between LBW podocytes and NBW podocytes, 107 genes (102 genes decreased in the LBW group and 5 genes increased in the LBW group) showed a False Discovery Rate (FDR) P -value (q-value) &lt; 0.05 between the two groups. Furthermore, IPA (Ingenuity® Pathway Analysis) by using these 107 genes was performed. As a result, five candidates for the upstream master regulator were identified: TNF, SIRT1, SH2B1, C5 and GDF15 as shown in the Figure. The downstream of all these pathways contain TCS2. This gene has been implicated in FSGS formation, as we recently reported [3].

CONCLUSION
It is possible that TCS2 is involved in the mechanism of FSGS formation in LBW-related nephropathy.
MO432: The ESRD Progression Delayed by Transplanting 3D Printed Omental Patch
Authors: Jo Hyunwoo , Choi Boyoung , Kim Boyun , Ryu Jina 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Renal fibrosis is one of the pathological hallmarks of chronic kidney disease (CKD) that can further progress to end-stage renal diseases (ESRD). Haemodialysis and kidney transplantation are the only feasible treatments of ESRD. In this study, a new treatment with autologous omental tissue was assessed to delay the progression of renal fibrosis. The omental tissue, a fatty structure, has been widely used in surgical procedures due to its immunologic, angiogenic characteristics and anti-fibrotic factors that enhance tissue repairs, cell proliferation and regeneration in a damaged tissue. Finally, we hypothesized that 3D printed omental patch can prevent renal fibrosis and help regenerate the damaged kidney in the animal model. The 3D printing can print cells and extracellular matrix uniformly to promote cell survival and differentiation during transplantation. The printing also can adjust the patch stiffness, providing a suitable environment for kidney recovery.

METHOD
Animal models of CKD were randomly divided into four groups: (A) sham-operated, (B) sham-operated + omental patch, (C) unilateral ureteral obstruction (UUO)-operated (Control) and (D) UUO-operated + omental patch. Micronized omental tissue as bio-ink was 3D printed in the form of a regular patch deformation. Then, the 3D printed omental patch was transplanted subcapsular layer of the kidney. All of the patches used in those groups were transplanted after 3 days of modelling. Kidneys were assessed histologically [Masson's Trichrome (MT), Sirius Red], by immunohistochemistry (α-SMA), molecular work (Bulk RNA sequencing, western blot) after 2 weeks of transplantation.

RESULTS
Omental patch treated group has been shown histologically less tubulointerstitial fibrosis and tubule atrophy by MT, Sirius Red staining. Particularly, the TGF beta mRNA expression level of the 3D printed omental treated group was decreased in the kidney, whereas the HGF level was significantly increased. Also, in terms of western blotting, pro-inflammatory and renal fibrosis markers (TGF-b, α-SMA) were all decreased. Moreover, in bulk RNA sequencing, we confirmed that STAT 3 kidney repair pathway and TF-beta signalling. From all those assessments we suggest that the induction of myofibroblasts and collagen deposition are all inhibited in the omental patch treated kidney model. The autologous 3D omental patch can prevent tubular atrophy and decreased tubulointerstitial fibrosis and this further delays the progression of ESRD.

CONCLUSION
The study demonstrated that the omental patch delays fibrosis and repairs tubule structure in the damaged kidney by providing protective effects to kidney disease. These studies indicate the feasibility of the use of the autologous omental patch in chronic kidney disease therapeutic effects and new treatment of renal replacement therapy (RRT).
MO433: Nitric-Oxide Precursors and Dimethylarginines as Risk Factors for Accelerated GFR Decline in the Non-Diabetic General Population
Authors: Balteskard Rinde Nikoline , Therese Enoksen Inger , Melsom Toralf , Fuskevag Ole-Martin , Odvar Eriksen Bjørn , Viljar Norvik Jon 
Published: May 2022

Abstract

BACKGROUND AND AIMS
Nitric oxide (NO) deficiency is associated with endothelial dysfunction, hypertension, vasoconstriction, atherosclerosis and chronic kidney disease (CKD) [1]. Reduced NO bioavailability is hypothesized to play a vital role in kidney function impairment and CKD. The worldwide prevalence of CKD is increasing and is a major contributor to morbidity and mortality. Differences in glomerular filtration rate (GFR) are only partly explained by conventional CKD risk factors [2]. Therefore, we investigated the association of serum levels of endogenous inhibitors of NO, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) and precursors of NO, arginine, citrulline and ornithine, with a decline in GFR and new-onset CKD.

METHOD
In a prospective cohort study of 1407 participants in the Renal Iohexol Clearance Survey (RENIS) without self-reported kidney disease, cardiovascular disease, or diabetes, GFR was measured repeatedly with iohexol clearance during a median follow-up time of 11 years [3]. GFR decline rates were analysed using a linear mixed model, new-onset CKD (GFR &lt; 60 ml/min/1.73 m2) with interval-censored Cox regression and accelerated GFR decline (the 10% with the steepest GFR decline) with logistic regression.

RESULTS
Neither ADMA nor arginine was associated with any outcomes. Higher SDMA was associated with a slower annual GFR decline and a lower arginine/dimethylarginine ratio with a steeper GFR decline. Higher levels of citrulline and ornithine were associated with accelerated GFR decline with an odds ratio (OR) of 1.43 [95% confidential interval (CI) 1.16–1.76] per standard deviation (SD) higher citrulline and an OR of 1.23 (95% CI 1.01–1.49) per SD increase in ornithine (Table 1). Higher citrulline was associated with new-onset CKD, with a hazard ratio of 1.31 (95% CI 1.05–1.63) per SD higher citrulline.

CONCLUSION
Associations between NO precursors and the outcomes suggest that NO-metabolism plays a significant role in the pathogenesis of age-related GFR decline, and the development of CKD in middle-aged people. Our findings warrant further investigation into the pathogenesis of NO-metabolism and the possible therapeutic targets and preventive measures.