bluuebunny/crossref_metadata_2025_split huggingface dataset:
The novel proteomic signature for cardiac allograft vasculopathy
Authors: Wei Dongmei , Trenson Sander , Van Keer Jan M. , Melgarejo Jesus , Cutsforth Ella , Thijs Lutgarde , He Tianlin , Latosinska Agnieszka , Ciarka Agnieszka , Vanassche Thomas , Van Aelst Lucas , Janssens Stefan , Van Cleemput Johan , Mischak Harald , Staessen Jan A. , Verhamme Peter , Zhang Zhen‐Yu 
Published: January 2022

AbstractAimsCardiac allograft vasculopathy (CAV) is the major long‐term complication after heart transplantation, leading to mortality and re‐transplantation. As available non‐invasive biomarkers are scarce for CAV screening, we aimed to identify a proteomic signature for CAV.Methods and resultsWe measured urinary proteome by capillary electrophoresis coupled with mass spectrometry in 217 heart transplantation recipients (mean age: 55.0 ± 14.4 years; women: 23.5%), including 76 (35.0%) patients with CAV diagnosed by coronary angiography. We randomly and evenly grouped participants into the derivation cohort (n = 108, mean age: 56.4 ± 13.8 years; women: 22.2%; CAV: n = 38) and the validation cohort (n = 109, mean age: 56.4 ± 13.8 years; women: 24.8%, CAV: n = 38), stratified by CAV. Using the decision tree‐based machine learning methods (extreme gradient boost), we constructed a proteomic signature for CAV discrimination in the derivation cohort and verified its performance in the validation cohort. The proteomic signature that consisted of 27 peptides yielded areas under the curve of 0.83 [95% confidence interval (CI): 0.75–0.91, P < 0.001] and 0.71 (95% CI: 0.60–0.81, P = 0.001) for CAV discrimination in the derivation and validation cohort, respectively. With the optimized threshold of 0.484, the sensitivity, specificity, and accuracy for CAV differentiation in the validation cohort were 68.4%, 73.2%, and 71.6%, respectively. With adjustment of potential clinical confounders, the signature was significantly associated with CAV [adjusted odds ratio: 1.31 (95% CI: 1.07–1.64) for per 0.1% increment in the predicted probability, P = 0.012]. Diagnostic accuracy significantly improved by adding the signature to the logistic model that already included multiple clinical risk factors, suggested by the integrated discrimination improvement of 9.1% (95% CI: 2.5–15.3, P = 0.005) and net reclassification improvement of 83.3% (95% CI: 46.7–119.5, P < 0.001). Of the 27 peptides, the majority were the fragments of collagen I (44.4%), collagen III (18.5%), collagen II (3.7%), collagen XI (3.7%), mucin‐1 (3.7%), xylosyltransferase 1 (3.7%), and protocadherin‐12 (3.7%). Pathway analysis performed in Reactome Pathway Database revealed that the multiple pathways involved by the signature were related to the pathogenesis of CAV, such as collagen turnover, platelet aggregation and coagulation, cell adhesion, and motility.ConclusionsThis pilot study identified and validated a urinary proteomic signature that provided a potential approach for the surveillance of CAV. These proteins might provide insights into CAV pathological processes and call for further investigation into personalized treatment targets.
Nafion® Coated Electropolymerised Flavanone‐based pH Sensor
Authors: Miranda Monica , Carvetta Clara , Sisodia Neel , Shirley Luke , Day Cristina D. , McGuinness Kay L. , Wadhawan Jay D. , Lawrence Nathan S. 
Published: January 2022

AbstractThis work summarizes the electrochemical response of flavanone carbon composite electrodes in comparison with Nafion®‐coated flavanone carbon composite electrodes, for use as voltammetric pH sensors in both buffered and low‐buffered media. Square wave voltammetric measurements suggest the peak potential achieved from the electrochemical polymerization after the electron‐proton oxidation responds with accuracy to buffered pH solutions for both coated and non‐coated electrodes, with a potential shift of 55.1 mV and 54.6 mV per pH unit respectively. However, a considerable improvement in stability, accuracy and sensitivity is provided by the proton‐transfer Nafion® layer in CO2 bubbled sea water. Furthermore, Nafion®‐coated flavanone carbon composite electrodes predicted a pH of 8.04 for the commercial seawater, which is in excellent agreement with the measured pH 8.05 value.
Adverse childhood experiences among a treatment‐seeking sample of adults with eating disorders
Authors: Rienecke Renee D. , Johnson Craig , Mehler Philip S. , Le Grange Daniel , Manwaring Jamie , Duffy Alan , McClanahan Susan , Blalock Dan V. 
Published: January 2022

AbstractObjectiveThe purpose of the current study was to examine the prevalence of adverse childhood experiences (ACEs) among adults with eating disorders (EDs), to assess whether experiencing a greater number of ACEs is associated with more severe ED psychopathology, and to determine whether ACEs predict treatment outcome.MethodParticipants were 1819 patients (88.5% female, ages 18–72) admitted to one of two treatment facilities at inpatient, residential, or partial hospitalisation levels of care. The Adverse Childhood Experiences Survey and the Eating Pathology Symptom Inventory (EPSI) were completed at admission and the EPSI at discharge.ResultsFemale patients reported higher ACEs than males (p = 0.03), and all diagnoses except avoidant/restrictive food intake disorder had significantly higher ACEs than patients with anorexia nervosa‐restricting type (AN‐R) (p's < 0.01). Across diagnoses, higher ACEs were associated with decreases in binge eating scores during treatment, but were not associated with changes in purging or restricting. Within diagnoses, higher ACEs scores were associated with decreases in purging for patients with AN‐R and increases in purging for patients with binge eating disorder.ConclusionsResults partially supported the hypothesis that higher ACEs would be associated with more severe ED psychopathology.
Phenolic profile and antioxidant activity of free/bound phenolic compounds of sesame and properties of encapsulated nanoparticles in different wall materials
Authors: Esmaeilzadeh Kenari Reza , Razavi Razie 
Published: January 2022

AbstractThis study aimed to evaluate the antioxidant activity of free and bound flavonoid or phenolic compounds extracted from the sesame seed (Oltan and Yekta varieties) as natural antioxidants and to demonstrate the properties of nanoparticles. The total phenolic content (TPC) of Oltan was higher (864.70 mg GAE/100 g seed) than that of Yekta (629.23 mg GAE/100 g seed). Oltan took up higher amounts of free (516.86 mg GAE/100 g seed) and bound (347.83 mg GAE/100 g seed) phenolics than Yekta. Also, the Yekta variety exhibited lower amounts of free (45.89 mg CE/100 g seed) and bound flavonoids (21.51 mg CE/100 g seed) and the total flavonoid content (TFC) (67.40 mg CE/100 g seed). Chlorogenic acid was the major phenolic compound present in the sesame seed. In both the DPPH and ferric reducing antioxidant power (FRAP) assays, the highest antioxidant activity was observed in the Oltan variety. Free phenolics showed the highest antioxidant activity, followed by bound phenolics, free and bound flavonoids. Therefore, free phenolics of the Oltan variety were encapsulated in Portulaca oleracea and Trigonella foenum‐graecum seed gums. All nanoparticles showed nanometric size from 236.1 to 680.7 nm, negative zeta potential from −35.4 to −18.3 mV, high encapsulation efficiency from 61.35% to 74.49%, and desirable polydispersity index (PDI) between 0.315 and 0.332. Higher release of phenolics and sedimentation rate were observed in phenolic compounds encapsulated in Khorfeh and Shanbalileh, respectively. The gradual release of phenolic compounds, as well as sedimentation rate of composite coating during 40 days of storage, demonstrated that nanoencapsulated phenolics of sesame within the composite gum coating could be used as natural antioxidants in food systems.
Inhibition of histamine accumulation by novel histamine‐degrading species of Staphylococcus sp. isolated from goats and sheep milk
Authors: Pashangeh Safoora , Shekarforoush Seyed Shahram , Aminlari Mahmoud , Hosseinzadeh Saeid , Nizet Victor , Dahesh Samira , Rahmdel Samane 
Published: January 2022

AbstractHistamine is an active amine compound that occurs in various fermented foods that may cause adverse effects on the human health. Certain microorganisms are able to degrade histamine by an oxidative deamination reaction. Therefore, the present study aimed to quantify histamine‐forming and/or ‐degrading activity of the isolates derived from milk of goat and sheep herds, in Iran, by the capillary zone electrophoresis (CZE) method; and we evaluated the molecular characteristics of staphylococcal isolates. Among 243 staphylococcal isolates, 29 histamine‐degrading bacteria were identified. One of these isolates, identified as Staph. epidermidis, No. 605, exhibited the highest activity compared to others, degrading available histamine to 58.33% within 24 h. By polymerase chain reaction (PCR) analysis, the isolate, No. 605 that exhibited remarkable histamine‐degrading activity lacked the genes encoding coagulase and DNase, nor did it harbor any of the five classical enterotoxin genes. This is the first report to show that seven Staphylococcus species, including Staph. chromogenes, Staph. aureus, Staph. haemolyticus, Staph. epidermidis, Staph. pseudintermedius, Staph. agnetis, and Staph. hyicus, were able to degrade histamine, which were hitherto not known to have this capacity. Therefore, histamine‐degrading activity is a definite criterion to introduce fermenting organisms able to decrease histamine content in different food products.
Prenatal air pollution exposure and neonatal health
Authors: Palma Alessandro , Petrunyk Inna , Vuri Daniela 
Published: January 2022

AbstractAir pollution has been shown to have adverse effects on many health outcomes including respiratory effects, cardiovascular effects, and mortality. However, evidence on the effects of prenatal exposure is still limited. We investigate the causal impact of prenatal exposure to air pollution on neonatal health in Italy in the 2000s. We exploit variation in rainfall shocks to instrument for non‐random air pollution exposure. Our empirical setting combines detailed information on mother's residential location from birth certificates with PM10 concentrations from air pollution monitors. Ten additional units in the average PM10 level (approximately one standard deviation) would decrease birth weight by about 0.5% and gestational age by 0.16%; it would increase the prevalence of low birth weight by 22% and of preterm birth by 16%. The effects are stronger in magnitude for third trimester exposure and for less educated mothers. These findings suggest that the health impacts of air pollution on newborns are unequally distributed in the population.
Notch‐triggered maladaptation of liver sinusoidal endothelium aggravates nonalcoholic steatohepatitis through endothelial nitric oxide synthase
Authors: Fang Zhi‐Qiang , Ruan Bai , Liu Jing‐Jing , Duan Juan‐Li , Yue Zhen‐Sheng , Song Ping , Xu Hao , Ding Jian , Xu Chen , Dou Guo‐Rui , Wang Lin 
Published: January 2022

Abstract

Background and Aims
Although NASH can lead to severe clinical consequences, including cirrhosis and hepatocellular carcinoma, no effective treatment is currently available for this disease. Increasing evidence indicates that LSECs play a critical role in NASH pathogenesis; however, the mechanisms involved in LSEC‐mediated NASH remain to be fully elucidated.

Approach and Results
In the current study, we found that LSEC homeostasis was disrupted and LSEC‐specific gene profiles were altered in methionine–choline‐deficient (MCD) diet‐induced NASH mouse models. Importantly, Notch signaling was found to be activated in LSECs of NASH mice. To then investigate the role of endothelial Notch in NASH progression, we generated mouse lines with endothelial‐specific Notch intracellular domain (NICD) overexpression or RBP‐J knockout to respectively activate or inhibit Notch signaling in endothelial cells. Notably, endothelial‐specific overexpression of the NICD accelerated LSEC maladaptation and aggravated NASH, whereas endothelial cell–specific inhibition of Notch signaling restored LSEC homeostasis and improved NASH phenotypes. Furthermore, we demonstrated that endothelial‐specific Notch activation exacerbated NASH by inhibiting endothelial nitric oxide synthase (eNOS) transcription, whereas administration of the pharmacological eNOS activator YC‐1 alleviated hepatic steatosis and lipid accumulation resulting from Notch activation. Finally, to explore the therapeutic potential of using Notch inhibitors in NASH treatment, we applied two gamma‐secretase inhibitors—DAPT and LY3039478—in an MCD diet‐induced mouse model of NASH, and found that both inhibitors effectively ameliorated hepatic steatosis, inflammation, and liver fibrosis.

Conclusions
Endothelial‐specific Notch activation triggered LSEC maladaptation and exacerbated NASH phenotypes in an eNOS‐dependent manner. Genetic and pharmacological inhibition of Notch signaling effectively restored LSEC homeostasis and ameliorated NASH progression.
Numerical simulation of MHD mixed convection flow of Al2O3–water nanofluid over two hot obstacles
Authors: Hosseini Abadshapoori M. , Saidi M. H. 
Published: January 2022

AbstractThe problem of cooling two hot blocks in a novel geometry using magnetohydrodynamic flow of Al2O3–water nanofluid has been studied utilizing a D2Q9 Lattice Boltzmann Model. While the Hartmann number (Ha) takes 0, 50, or 100 values, the Richardson number (Ri) varies between 0.02 and 20. Four variations of the geometry are selected. The gravity angle is set to be either , , or . Results reveal that the Nusselt number (Nu) increases as Ri increases for all cases. Furthermore, the Hartmann number has a deteriorating effect on the Nusselt number except for low Ri numbers. In addition, the results indicate that while the geometrical configuration is having a considerable impact on the average Nusselt number at low and high Richardson numbers, it has a negligible effect at the mixed convection flow. The best angle for the gravitational force is also between 0 and . A new correlation for the Nu number based on all parameters is also presented.
Simulated event‐scale flow and sediment generation responses to agricultural land cover change in lowland UK catchments
Authors: Escobar‐Ruiz Veronica , Smith Hugh G. , Macdonald Neil , Peñuela Andres 
Published: January 2022

AbstractAgricultural land use can increase runoff and erosion leading to detrimental downstream impacts. This paper examines the impact of agricultural land cover change on runoff and sediment generation at event scales using a model‐based approach. SHETRAN, a physically based, spatially distributed model, was applied in two southwest England catchments to represent: (a) changes in the land cover (cropland extent and spatial arrangement), (b) changes in crop type and (c) use of riparian buffer strips. A total of 84 simulated events within a 4‐year period were used to quantify impacts on flow and sediment generation. We found past changes in land cover resulted in significant differences in sediment yield (p < 0.05). Linear regression showed an increase in flow and sediment yield proportional to increases in arable crop area (p < 0.001). The spatial arrangement of cropped fields and riparian buffer strips produced no significant differences in event flow or sediment yield (p > 0.05). However, buffer strip scenarios compared with the base run showed sediment load reductions for specific events, up to 20% and 15% for woodland and grass riparian buffers, respectively. When comparing crop types with and without the use of post‐harvest cover crops, we observed non‐significant differences for event flow and sediment yield. However, large reductions in modelled sediment yields occurred for some events (e.g., up to 60% for winter cereals, 50% for maize and 74% for spring cereals). For these scenarios, examination of rainfall event magnitude emphasized the importance of ground cover in mitigating soil erosion for maize and spring cereals, but not for winter cereals. Our findings indicate that significant changes in sediment delivery at the event scale over a multi‐year period are associated with variations in cropland extent and crop types, depending on rainfall magnitude, but not on the spatial arrangement of cropped fields or the use of riparian buffer strips.
Polymerizing small molecular acceptors for efficient all‐polymer solar cells
Authors: Kong Yuxin , Li Yuxiang , Yuan Jianyu , Ding Liming 
Published: January 2022

AbstractAll‐polymer solar cells (all‐PSCs) have received attention due to their morphological stability under thermal and mechanical stresses. Currently, the highest reported power conversion efficiency of all‐PSCs is over 17%, achieved by utilizing polymerized small molecular acceptors (PSMAs). However, the need for higher regiospecificity to avoid forming isomers during polymerization of SMAs still challenges the further applications of all‐PSCs. From this perspective, we focus on some recent studies and highlight the importance of controlling the regioregularity of PSMAs. In particular, integrating PSMAs with regioregularity endows the polymer acceptors with good absorption, superior backbone ordering, and optimal blend morphology compared with those obtained from regiorandom one. Moreover, the distinctive features that are derived from these regioregular PSMAs, such as the possibility of repeatable synthesis and reproducible device performance, herald a brighter future for scaling‐up and commercializing all‐PSCs. We expect this integrated strategy will inspire researchers to devote more efforts to further narrow the efficiency gap between the PSCs based on SMAs and PSMAs. Finally, we discuss the existing challenges and future prospects of PSMAs as new platform for further advancing all‐PSCs.
Efficacy of the Developmental Trauma and Attachment Program in a residential setting
Authors: Lenz A. Stephen , Buckwalter Karen Doyle , Pester Danielle A. , Green Kelly , Reed Debbie 
Published: January 2022

AbstractThis evaluation inspected the degree of symptom improvement associated with the Developmental Trauma and Attachment Program for decreasing symptoms of posttraumatic stress disorder (PTSD) among youth in residential treatment following complex trauma scenarios associated with domestic and international adoption/foster care. Participants were 61 youth (Mage = 13.23 years) who were predominately born in the United States (n = 41) with 20 identifying with international domiciles. Results indicated statistically significant decreases in symptoms from admission to discharge associated with medium‐effect sizes across PTSD symptoms. Nearly one in three participants reported clinically significant changes associated with treatment, whereas two in five reported improvements but not in the clinically significant range. Female participants reported greater decreases in negative cognitions; however, no other differences in treatment response based on participant characteristics were detected. Limitations of the generalization of findings and recommendations for future researchers are provided.
Combined strategy of knowledge‐based rule selection and historical data percentile‐based range determination to improve an autoverification system for clinical chemistry test results
Authors: Zhu Jing , Wang Hao , Wang Beili , Hao Xiaoke , Cui Wei , Duan Yong , Zhang Yi , Ming Liang , Zhou Yingchun , Ding Haitao , Ou Hongling , Lin Weiwei , Lu Liu , Shang Yuanjiang , Yang Yong , Liang Xianming , Ma Jiangtao , Sun Wenhua , Chen Te , Han Guang , Han Meng , Yu Weiting , Pan Baishen , Guo Wei 
Published: January 2022

AbstractBackgroundCurrent autoverification, which is only knowledge‐based, has low efficiency. Regular historical data analysis may improve autoverification range determination. We attempted to enhance autoverification by selecting autoverification rules by knowledge and ranges from historical data. This new system was compared with the original knowledge‐based system.MethodsNew types of rules, extreme values, and consistency checks were added and the autoverification workflow was rearranged to construct a framework. Criteria for creating rules for extreme value ranges, limit checks, consistency checks, and delta checks were determined by analyzing historical Zhongshan laboratory data. The new system's effectiveness was evaluated using pooled data from 20 centers. Efficiency improvement was assessed by a multicenter process.ResultsEffectiveness was evaluated by the true positive rate, true negative rate, and overall consistency rate, as compared to manual verification, which were 77.55%, 78.53%, and 78.3%, respectively for the new system. The original overall consistency rate was 56.2%. The new pass rates, indicating efficiency, were increased by 19%‒51% among hospitals. Further customization using individualized data increased this rate.ConclusionsThe improved system showed a comparable effectiveness and markedly increased efficiency. This transferable system could be further improved and popularized by utilizing historical data from each hospital.
A call for unity in DEIJ efforts using a proposed framework for Education, Recruitment, Retainment, Research, and Active outreach (ERA) for genetic counselors in the United States
Authors: Mercado Joanna , Lawrence Janette Z. , Moore Michelle , Neogi Arpita , Vig Hetal 
Published: January 2022

AbstractGenetic counselors, like many other healthcare providers, play a vital role in genomic health care. As a profession, we, along with our colleagues and students, have recognized the need to improve and incorporate diversity, equity, inclusion, and justice (DEIJ) within our daily ways of practice to help create access to genomic technologies. In order to create systemic change and focus on unity, open communication, and transparency, we introduce a suggested framework called ERA (Education, Recruitment, Retainment, Research, and Active Outreach). This framework would benefit a genetic counselor throughout various stages of their career, from student to practicing genetic counselor, and can be broadly applied to all specialties of genomic medicine. Different iterations of DEIJ efforts have arisen in the National Society of Genetic Counselors (NSGC), from the D&I (Diversity and Inclusion) Task Force to the J.E.D.I (justice, equity, diversity, and inclusion) committee. The lack of recorded history of these previous efforts and the lack of communication between current DEIJ organizations is one of the many reasons the ERA framework demands unity and transparency to achieve sustainable positive change. Genetic counselors must unite and work collaboratively to conduct and promote DEIJ efforts, so the benefits of genomic medicine can be realized by all.