bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonlocal nonlinear phononics
Authors: Henstridge M. , Först M. , Rowe E. , Fechner M. , Cavalleri A. 
Published: March 2022

AbstractNonlinear phononics relies on the resonant optical excitation of infrared-active lattice vibrations to induce targeted structural deformations in solids. This form of dynamical crystal structure design has been applied to control the functional properties of many complex solids, including magnetic materials, superconductors and ferroelectrics. However, phononics has so far been restricted to protocols in which structural deformations occur within the optically excited volume, sometimes resulting in unwanted heating. Here, we extend nonlinear phononics to propagating polaritons, spatially separating the functional response from the optical drive. We use mid-infrared optical pulses to resonantly drive a phonon at the surface of ferroelectric LiNbO3. Time-resolved stimulated Raman scattering reveals that the ferroelectric polarization is reduced over the entire 50 µm depth of the sample, far beyond the micrometre depth of the evanescent phonon field. We attribute this effect to the anharmonic coupling between the driven mode and a polariton that propagates into the material. For high excitation amplitudes, we reach a regime in which the ferroelectric polarization is reversed, as revealed by a sign change in the Raman tensor coefficients of all the polar modes.
A method for direct estimation of left ventricular global longitudinal strain rate from echocardiograms
Authors: Meyers Brett A. , Brindise Melissa C. , Kutty Shelby , Vlachos Pavlos P. 
Published: March 2022

AbstractWe present a new method for measuring global longitudinal strain and global longitudinal strain rate from 2D echocardiograms using a logarithmic-transform correlation (LTC) method. Traditional echocardiography strain analysis depends on user inputs and chamber segmentation, which yield increased measurement variability. In contrast, our approach is automated and does not require cardiac chamber segmentation and regularization, thus eliminating these issues. The algorithm was benchmarked against two conventional strain analysis methods using synthetic left ventricle ultrasound images. Measurement error was assessed as a function of contrast-to-noise ratio (CNR) using mean absolute error and root-mean-square error. LTC showed better agreement to the ground truth strain $${({\varvec{R}}}^{2}=0.91)$$

(

R

2

=
0.91
)

and ground truth strain rate $${({\varvec{R}}}^{2}=0.85)$$

(

R

2

=
0.85
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compared with agreement to ground truth for two block-matching speckle tracking algorithms (one based on sum of square difference and the other on Fourier transform correlation; strain $${({\varvec{R}}}^{2}=0.70)$$

(

R

2

=
0.70
)

, strain rate $${({\varvec{R}}}^{2}=0.70)$$

(

R

2

=
0.70
)

). A 200% increase in strain measurement accuracy was observed compared to the conventional algorithms. Subsequently, we tested the method using a 53-subject clinical cohort (20 subjects diseased with cardiomyopathy, 33 healthy controls). Our method distinguished between normal and abnormal left ventricular function with an AUC = 0.89, a 5% improvement over the conventional GLS algorithms.
Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury
Authors: Hong Yu-ri , Lee Eun-hee , Park Ki-su , Han Mun , Kim Kyoung-Tae , Park Juyoung 
Published: March 2022

AbstractSpinal cord injury (SCI) is associated with limited functional recovery. Despite advances in neuroscience, realistic therapeutic treatments for SCI remain unavailable. In this study, the effects of non-invasive ultrasound (US) treatment on behavior and inflammatory responses were evaluated in a rat model of SCI. Adult female Sprague–Dawley rats were subjected to spinal cord contusion injury. Two different US parameters (SCIU5: 5% and SCIU40: 40% duty cycle) were applied, and their effects on behavioral recovery after SCI were quantified. Tissue and neuronal responses were detected. Immunofluorescence was used to detect inflammatory markers. In the rat model of SCI, motor function was more effectively restored, and the lesion cavity area was smaller in the SCIU5 group. Furthermore, the SCIU5 protocol elicited an anti-inflammatory response at the injury site by reducing degenerative FJC-labeled neurons, macrophage/microglia activation, and infiltration. Thus, the lesion area decreased, and tissue density increased. Meanwhile, the SCIU40 protocol did not improve motor function or induce an anti-inflammatory response at the injury site. The SCIU5 protocol effectively accelerated the rate of improved exercise performance in the rat model while reducing inflammation. Accordingly, appropriate US stimulation may represent a promising treatment modality for SCI with beneficial anti-inflammatory effects.
Shared genetic loci for body fat storage and adipocyte lipolysis in humans
Authors: Kulyté Agné , Lundbäck Veroniqa , Arner Peter , Strawbridge Rona J. , Dahlman Ingrid 
Published: March 2022

AbstractTotal body fat and central fat distribution are heritable traits and well-established predictors of adverse metabolic outcomes. Lipolysis is the process responsible for the hydrolysis of triacylglycerols stored in adipocytes. To increase our understanding of the genetic regulation of body fat distribution and total body fat, we set out to determine if genetic variants associated with body mass index (BMI) or waist-hip-ratio adjusted for BMI (WHRadjBMI) in genome-wide association studies (GWAS) mediate their effect by influencing adipocyte lipolysis. We utilized data from the recent GWAS of spontaneous and isoprenaline-stimulated lipolysis in the unique GENetics of Adipocyte Lipolysis (GENiAL) cohort. GENiAL consists of 939 participants who have undergone abdominal subcutaneous adipose biopsy for the determination of spontaneous and isoprenaline-stimulated lipolysis in adipocytes. We report 11 BMI and 15 WHRadjBMI loci with SNPs displaying nominal association with lipolysis and allele-dependent gene expression in adipose tissue according to in silico analysis. Functional evaluation of candidate genes in these loci by small interfering RNAs (siRNA)-mediated knock-down in adipose-derived stem cells identified ZNF436 and NUP85 as intrinsic regulators of lipolysis consistent with the associations observed in the clinical cohorts. Furthermore, candidate genes in another BMI-locus (STX17) and two more WHRadjBMI loci (NID2, GGA3, GRB2) control lipolysis alone, or in conjunction with lipid storage, and may hereby be involved in genetic control of body fat. The findings expand our understanding of how genetic variants mediate their impact on the complex traits of fat storage and distribution.
Seasonal variation in space use and territoriality in a large mammal (Sus scrofa)
Authors: Schlichting Peter E. , Boughton Raoul K. , Anderson Wes , Wight Bethany , VerCauteren Kurt C. , Miller Ryan S. , Lewis Jesse S. 
Published: March 2022

AbstractAn individual’s spatial behavior is shaped by social and environmental factors and provides critical information about population processes to inform conservation and management actions. Heterogeneity in spatial overlap among conspecifics can be evaluated using estimates of home ranges and core areas and used to understand factors influencing space use and territoriality. To understand and test predictions about spatial behavior in an invasive large mammal, the wild pig (Sus scrofa), we examined variation in space use between sexes and seasons. We predicted that if animals were territorial that there would be a reduction in space-use overlap when comparing overlap of home ranges (HR–HR), to home ranges and core areas (HR–CA), and in-turn between core areas (CA–CA). Home ranges and core areas were estimated for 54 wild pigs at Buck Island Ranch, FL from GPS telemetry data. Overlap indices were calculated to estimate the strength (space-use overlap) and number of potential interactions within three wet seasons (June–October) and two dry seasons (December–April). Among sexes, home range size did not vary seasonally, and males exhibited larger home ranges compared to females (M = 10.36 ± 0.79 km2 (± SE), F = 3.21 ± 0.16 km2). Strength of overlap varied by season with wild pig home ranges overlapping more during the dry season. Males interacted with a greater number of individuals of both sexes, compared to females, and exhibited greater strength of overlap during the dry season. Consistent with our predictions, wild pigs appeared to exhibit territorial behavior, where strength of overlap decreased when comparing HR–HR to HR–CA and HR–CA to CA–CA. Our framework can be used to understand patterns of space use and territoriality in populations, which has important implications in understanding intraspecific interactions and population processes, such as how pathogens and parasites might spread within and among populations.
A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
Authors: Kim Benjamin B. , Wu Haodi , Hao Yukun A. , Pan Michael , Chavarha Mariya , Zhao Yufeng , Westberg Michael , St-Pierre François , Wu Joseph C. , Lin Michael Z. 
Published: March 2022

AbstractA ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in the presence of sample motion. We performed combinatorial multi-site mutagenesis on a cyan-excitable red fluorescent protein to create the bright and monomeric mCyRFP3, which proved to be uniquely non-perturbing when fused to the GEVI ASAP3. The green/red ratio from ASAP3-mCyRFP3 (ASAP3-R3) reported voltage while correcting for motion artifacts, allowing the visualization of membrane voltage changes in contracting cardiomyocytes and throughout the cell cycle of motile cells.
Modeling solubility of CO2–N2 gas mixtures in aqueous electrolyte systems using artificial intelligence techniques and equations of state
Authors: Nakhaei-Kohani Reza , Taslimi-Renani Ehsan , Hadavimoghaddam Fahime , Mohammadi Mohammad-Reza , Hemmati-Sarapardeh Abdolhossein 
Published: March 2022

AbstractDetermining the solubility of non-hydrocarbon gases such as carbon dioxide (CO2) and nitrogen (N2) in water and brine is one of the most controversial challenges in the oil and chemical industries. Although many researches have been conducted on solubility of gases in brine and water, very few researches investigated the solubility of power plant flue gases (CO2–N2mixtures) in aqueous solutions. In this study, using six intelligent models, including Random Forest, Decision Tree (DT), Gradient Boosting-Decision Tree (GB-DT), Adaptive Boosting-Decision Tree (AdaBoost-DT), Adaptive Boosting-Support Vector Regression (AdaBoost-SVR), and Gradient Boosting-Support Vector Regression (GB-SVR), the solubility of CO2–N2mixtures in water and brine solutions was predicted, and the results were compared with four equations of state (EOSs), including Peng–Robinson (PR), Soave–Redlich–Kwong (SRK), Valderrama–Patel–Teja (VPT), and Perturbed-Chain Statistical Associating Fluid Theory (PC-SAFT). The results indicate that the Random Forest model with an average absolute percent relative error (AAPRE) value of 2.8% has the best predictions. The GB-SVR and DT models also have good precision with AAPRE values of 6.43% and 7.41%, respectively. For solubility of CO2present in gaseous mixtures in aqueous systems, the PC-SAFT model, and for solubility of N2, the VPT EOS had the best results among the EOSs. Also, the sensitivity analysis of input parameters showed that increasing the mole percent of CO2in gaseous phase, temperature, pressure, and decreasing the ionic strength increase the solubility of CO2–N2mixture in water and brine solutions. Another significant issue is that increasing the salinity of brine also has a subtractive effect on the solubility of CO2–N2mixture. Finally, the Leverage method proved that the actual data are of excellent quality and the Random Forest approach is quite reliable for determining the solubility of the CO2–N2gas mixtures in aqueous systems.
Deprogramming metabolism in pancreatic cancer with a bi-functional GPR55 inhibitor and biased β2 adrenergic agonist
Authors: Wnorowski Artur , Dudzik Danuta , Bernier Michel , Wójcik Jakub , Keijzers Guido , Diaz-Ruiz Alberto , Mazur Karolina , Zhang Yongqing , Han Haiyong , Scheibye-Knudsen Morten , Jozwiak Krzysztof , Barbas Coral , Wainer Irving W. 
Published: March 2022

AbstractMetabolic reprogramming contributes to oncogenesis, tumor growth, and treatment resistance in pancreatic ductal adenocarcinoma (PDAC). Here we report the effects of (R,S′)-4′-methoxy-1-naphthylfenoterol (MNF), a GPR55 antagonist and biased β2-adrenergic receptor (β2-AR) agonist on cellular signaling implicated in proliferation and metabolism in PDAC cells. The relative contribution of GPR55 and β2-AR in (R,S′)-MNF signaling was explored further in PANC-1 cells. Moreover, the effect of (R,S′)-MNF on tumor growth was determined in a PANC-1 mouse xenograft model. PANC-1 cells treated with (R,S′)-MNF showed marked attenuation in GPR55 signal transduction and function combined with increased β2-AR/Gαs/adenylyl cyclase/PKA signaling, both of which contributing to lower MEK/ERK, PI3K/AKT and YAP/TAZ signaling. (R,S′)-MNF administration significantly reduced PANC-1 tumor growth and circulating l-lactate concentrations. Global metabolic profiling of (R,S′)-MNF-treated tumor tissues revealed decreased glycolytic metabolism, with a shift towards normoxic processes, attenuated glutamate metabolism, and increased levels of ophthalmic acid and its precursor, 2-aminobutyric acid, indicative of elevated oxidative stress. Transcriptomics and immunoblot analyses indicated the downregulation of gene and protein expression of HIF-1α and c-Myc, key initiators of metabolic reprogramming in PDAC. (R,S′)-MNF treatment decreased HIF-1α and c-Myc expression, attenuated glycolysis, shifted fatty acid metabolism towards β-oxidation, and suppressed de novo pyrimidine biosynthesis in PANC-1 tumors. The results indicate a potential benefit of combined GPR55 antagonism and biased β2-AR agonism in PDAC therapy associated with the deprogramming of altered cellular metabolism.
Evaluating COVID-19 control measures in mass gathering events with vaccine inequalities
Authors: Al-Shaery Ali M. , Hejase Bilal , Tridane Abdessamad , Farooqi Norah S. , Al Jassmi Hamad 
Published: March 2022

AbstractWith the increasing global adoption of COVID-19 vaccines, limitations on mass gathering events have started to gradually loosen. However, the large vaccine inequality recorded among different countries is an important aspect that policymakers must address when implementing control measures for such events. In this paper, we propose a model for the assessment of different control measures with the consideration of vaccine inequality in the population. Two control measures are considered: selecting participants based on vaccine efficacy and restricting the event capacity. We build the model using agent-based modeling to capture the spatiotemporal crowd dynamics and utilize a genetic algorithm to assess the control strategies. This assessment is based on factors that are important for policymakers such as disease prevalence, vaccine diversity, and event capacity. A quantitative evaluation of vaccine diversity using the Simpson’s Diversity Index is also provided. The Hajj ritual is used as a case study. We show that strategies that prioritized lowering the prevalence resulted in low event capacity but facilitated vaccine diversity. Moreover, strategies that prioritized diversity resulted in high infection rates. However, increasing the prioritization of participants with high vaccine efficacy significantly decreased the disease prevalence. Strategies that prioritized ritual capacity did not show clear trends.
Changes of the retinal and choroidal vasculature in cerebral small vessel disease
Authors: Geerling Clara F. , Terheyden Jan H. , Langner S. Magdalena , Kindler Christine , Keil Vera C. , Turski Christopher A. , Turski Gabrielle N. , Wintergerst Maximillian W. M. , Petzold Gabor C. , Finger Robert P. 
Published: March 2022

AbstractCerebral small vessel disease (CSVD) is associated with changes in the retinal vasculature which can be assessed non-invasively with much higher resolution than the cerebral vasculature. To detect changes at a microvascular level, we used optical coherence tomography angiography which resolves retinal and choroidal vasculature. Participants with CSVD and controls were included. White matter lesions were determined on magnetic resonance imaging (MRI). The retinal and choroidal vasculature were quantified using swept-source optical coherence tomography angiography. Data were analysed using linear regression. We included 30 participants (18 females; patients, n = 20; controls, n = 10) with a mean age of 61 ± 10 years. Patients had a higher mean white matter lesion index and number of lesions than controls (p ≤ 0.002). The intraindividual deviation of choriocapillaris reflectivity differed significantly between age-matched patients (0.234 ± 0.012) and controls (0.247 ± 0.011; p = 0.029). Skeleton density of the deep retinal capillaries was significantly associated with the number of lesions on MRI (β = − 5.3 × 108, 95%-confidence interval [− 10.3 × 108; − 0.2 × 108]) when controlling for age. The choroidal microvasculature and the deep retinal vascular plexus, as quantified by optical coherence tomography angiography, are significantly altered in CSVD. The value of these findings in diagnosing or monitoring CSVD need to be assessed in future studies.
Characterisation of the volatile profile of microalgae and cyanobacteria using solid-phase microextraction followed by gas chromatography coupled to mass spectrometry
Authors: Moran Lara , Bou Gemma , Aldai Noelia , Ciardi Martina , Morillas-España Ainoa , Sánchez-Zurano Ana , Barron Luis Javier R. , Lafarga Tomas 
Published: March 2022

AbstractMicroalgae and microalgae-derived ingredients are one of the top trends in the food industry. However, consumers’ acceptance and purchase intention of a product will be largely affected by odour and flavour. Surprisingly, the scientific literature present a very limited number of studies on the volatile composition of microalgae and cyanobacteria. In order to fill the gap, the main objective of the present study was to elucidate the volatile composition of seven microalgal and cyanobacterial strains from marine and freshwaters, with interest for the food industry while establishing its potential impact in odour. Among the seven selected strains, Arthrospira platensis showed the highest abundance and chemical diversity of volatile organic compounds (VOCs). Aldehydes, ketones, and alcohols were the families with the highest diversity of individual compounds, except in Arthrospira platensis and Scenedesmus almeriensis that showed a profile dominated by branched hydrocarbons. Marine strains presented a higher abundance of sulfur compounds than freshwater strains, while the ketones individual profile seemed to be more related to the taxonomical domain. The results of this study indicate that the VOCs composition is mainly driven by the individual strain although some volatile profile characteristics could be influenced by both environmental and taxonomical factors.
Link between the EZH2 noncanonical pathway and microtubule organization center polarization during early T lymphopoiesis
Authors: Deshayes Frederique , Fradet Magali , Kaminski Sandra , Viguier Mireille , Frippiat Jean-Pol , Ghislin Stephanie 
Published: March 2022

AbstractEZH2 plays an essential role at the β-selection checkpoint of T lymphopoiesis by regulating histone H3 lysine 27 trimethylation (H3K27me3) via its canonical mode of action. Increasing data suggest that EZH2 could also regulate other cellular functions, such as cytoskeletal reorganization, via its noncanonical pathway. Consequently, we investigated whether the EZH2 noncanonical pathway could be involved in early T-cell maturation, which requires cell polarization. We observed that EZH2 localization is tightly regulated during the early stages of T-cell development and that EZH2 relocalizes in the nucleus of double-negative thymocytes enduring TCRβ recombination and β-selection processes. Furthermore, we observed that EZH2 and EED, but not Suz12, colocalize with the microtubule organization center (MTOC), which might prevent its inappropriate polarization in double negative cells. In accordance with these results, we evidenced the existence of direct or indirect interaction between EED and α-tubulin. Taken together, these results suggest that the EZH2 noncanonical pathway, in association with EED, is involved in the early stages of T-cell maturation.
Association of physical activity and positive thinking with global sleep quality
Authors: Chen Morgan , He Zhonghui , Zhang Zhanjia , Chen Weiyun 
Published: March 2022

AbstractThis study examined the association of different intensity levels of physical activity and positive thinking with a global sleep quality among college students. The research question was: to what degree were the different intensity levels of physical activity and positive thinking significantly associated with the global sleep quality among college students? We recruited students, who enrolled in regular physical education classes during a fall semester at a major public university. 553 students signed the consent form and indicated their voluntary participation in this study. The final data set for analysis consisted of 403 college students with the mean age of 19.01 years ± 1.559 years (217 males vs. 186 females) based on the results of data screening. They completed three questionnaires: International Physical Activity Questionnaire (IPAQ), Pittsburgh Sleep Quality Index (PSQI), and Positive Thinking Scale (PTS) during a regular physical education class. The multiple regression model revealed that vigorous-intensity physical activity, positive thinking, and negative thinking were collectively and individually associated with the Global PSQI sleep quality (F = 19.389, p = .000), explaining 12.8% of the total variance in the Global PSQI sleep quality for the total sample. College students’ engaging in vigorous intensity level of physical activity, and having a good level of positive thinking and a low level of negative thinking were both collectively and individually linked to the Global PSQI sleep quality.
The influence of the pH on the incorporation of caffeic acid into biomimetic membranes and cancer cells
Authors: Naumowicz Monika , Kusaczuk Magdalena , Zając Marcin , Jabłońska-Trypuć Agata , Mikłosz Agnieszka , Gál Miroslav , Worobiczuk Mateusz , Kotyńska Joanna 
Published: March 2022

AbstractCaffeic acid (CA) is a phenolic compound synthesized by all plant species. It constitutes the main hydroxycinnamic acid found in human diet and presents a variety of beneficial effects including anticancer activity. Current data suggests essential role of the interplay between anticancer drugs and the cell membrane. Given this, biophysical interactions between CA and cancer cells or biomimetic membranes were investigated. Glioblastoma cell line U118MG and colorectal adenocarcinoma cell line DLD-1, as well as lipid bilayers and liposomes, were used as in vitro models. Electrophoretic light scattering was used to assess the effect of CA on the surface charge of cancer cells and liposomal membranes. Electrochemical impedance spectroscopy was chosen to evaluate CA-dependent modulatory effect on the electrical capacitance and electrical resistance of the bilayers. Our results suggest that CA fulfills physicochemical criteria determining drug-like properties of chemical compounds, and may serve as a potential cytostatic agent in cancer treatment.