bluuebunny/crossref_metadata_2025_split huggingface dataset:
Andrographolide protects bone marrow mesenchymal stem cells against glucose and serum deprivation under hypoxia via the NRF2 signaling pathway
Authors: Sun Yanting , Xu Hao , Tan Bin , Yi Qin , Liu Huiwen , Chen Tangtian , Xiang Han , Wang Rui , Xie Qiumin , Tian Jie , Zhu Jing 
Published: July 2022

AbstractBackgroundBone marrow mesenchymal stem cell (BMSCs) therapy is an important cell transplantation strategy in the regenerative medicine field. However, a severely ischemic microenvironment, such as nutrient depletion and hypoxia, causes a lower survival rate of transplanted BMSCs, limiting the application of BMSCs. Therefore, improving BMSCs viability in adverse microenvironments is an important means to improve the effectiveness of BMSCs therapy.ObjectiveTo illustrate the protective effect of andrographolide (AG) against glucose and serum deprivation under hypoxia (1% O2) (GSDH)-induced cell injury in BMSCs and investigate the possible underlying mechanisms.MethodsAn in vitro primary rat BMSCs cell injury model was established by GSDH, and cellular viability, proliferation and apoptosis were observed after AG treatment under GSDH. Reactive oxygen species levels and oxidative stress-related genes and proteins were measured by flow cytometry, RT-qPCR and Western blotting. Mitochondrial morphology, function and number were further assessed by laser confocal microscopy and flow cytometry.ResultsAG protected BMSCs against GSDH-induced cell injury, as indicated by increases in cell viability and proliferation and mitochondrial number and decreases in apoptosis and oxidative stress. The metabolic status of BMSCs was changed from glycolysis to oxidative phosphorylation to increase the ATP supply. We further observed that the NRF2 pathway was activated by AG, and treatment of BMSCs with a specific NRF2 inhibitor (ML385) blocked the protective effect of AG.ConclusionOur results suggest that AG is a promising agent to improve the therapeutic effect of BMSCs.
The Campbell Collaboration’s systematic review of school-based anti-bullying interventions does not meet mandatory methodological standards
Authors: Littell Julia H. , Gorman Dennis M. 
Published: July 2022

Abstract
Background
Many published reviews do not meet the widely accepted PRISMA standards for systematic reviews and meta-analysis. Campbell Collaboration and Cochrane reviews are expected to meet even more rigorous standards, but their adherence to these standards is uneven. For example, a newly updated Campbell systematic review of school-based anti-bullying interventions does not appear to meet many of the Campbell Collaboration’s mandatory methodological standards.

Issues
In this commentary, we document methodological problems in the Campbell Collaboration's new school-based anti-bullying interventions review, including (1) unexplained deviations from the protocol; (2) inadequate documentation of search strategies; (3) inconsistent reports on the number of included studies; (4) undocumented risk of bias ratings; (5) assessments of selective outcome reporting bias that are not transparent, not replicable, and appear to systematically underestimate risk of bias; (6) unreliable assessments of risk of publication bias; (7) use of a composite scale that conflates distinct risks of bias; and (8) failure to consider issues related to the strength of the evidence and risks of bias in interpreting results and drawing conclusions. Readers who are unaware of these problems may place more confidence in this review than is warranted. Campbell Collaboration editors declined to publish our comments and declined to issue a public statement of concern about this review.

Conclusions
Systematic reviews are expected to use transparent methods and follow relevant methodological standards. Readers should be concerned when these expectations are not met, because transparency and rigor enhance the trustworthiness of results and conclusions. In the tradition of Donald T. Campbell, there is need for more public debate about the methods and conclusions of systematic reviews, and greater clarity regarding applications of (and adherence to) published standards for systematic reviews.
Benchmarking public policies to create healthy food environments compared to best practice: the Healthy Food Environment Policy Index in Guatemala
Authors: Sánchez-Nóchez Carmen María , Ramirez-Zea Manuel , Vandevijvere Stefanie , Kroker-Lobos María Fernanda 
Published: July 2022

Abstract
Background
Benchmarking the implementation of healthy food environment public policies against international best practices may accelerate the government response to prevent obesity and non-communicable diseases (NCDs) in the countries. The aim of the study was to determine the extent of food environment policy implementation in Guatemala and to identify and prioritize actions for the government to accelerate their implementation.

Methods
The INFORMAS Healthy Food Environment Policy Index (Food-EPI from the International Network for Food and Obesity/NCDs Research, Monitoring and Action Support) was used. Evidence of implementation for 50 good practice indicators within the seven food policies and six infrastructure support domains was compiled, and subsequently validated by Guatemalan government officials. A national civil society expert panel on public health and nutrition performed an online assessment of the implementation of healthy food environment policies against best international practices. The level of agreement among evaluators was measured using the Gwet second order agreement coefficient (AC2). The expert panel recommended actions for each indicator during on-site workshops and those actions were prioritized by importance and achievability.

Results
The expert panel rated implementation at zero for 26% of the indicators, very low for 28% of indicators, low for 42%, and medium for 4% of indicators (none were rated high). Indicators at medium implementation were related to the use of evidence for developing policies and ingredient list/nutrition information panels on packaged foods. Seventy-seven actions were recommended prioritizing the top 10 for immediate action. The Gwet AC2 was 0.73 (95% CI 0.67–0.80), indicating a good concordance among experts.

Conclusions
In the Food-EPI of Guatemala, almost all indicators of good practice had a low or less level of implementation. The expert panel proposed 12 priority actions to accelerate policy implementation to tackle obesity and NCDs in the country.
Serum and plasma levels of brain-derived neurotrophic factor in individuals with eating disorders (EDs): a systematic review and meta-analysis
Authors: Shobeiri Parnian , Bagherieh Sara , Mirzayi Parsa , Kalantari Amirali , Mirmosayyeb Omid , Teixeira Antônio L. , Rezaei Nima 
Published: July 2022

Abstract
Background
Brain-derived neurotrophic factor (BDNF) is essential for neuronal survival, differentiation, development, and plasticity. Evidence suggests that fluctuations in peripheral levels (i.e., plasma or serum) of BDNF are associated with eating behaviors. Nevertheless, the findings are inconsistent. The purpose of this study is to determine if serum or plasma levels of BDNF are altered in individuals with eating disorders (EDs) compared to controls.

Methods
We conducted a systematic search of the core electronic medical databases from inception to March 2022 and identified observational studies that compared individuals with EDs to controls without EDs on serum or plasma levels of BDNF. R version 4.0.4 was used for all visualizations and calculations.

Results
The current meta-analysis comprised 15 studies that fulfilled the inclusion criteria. Subjects with EDs (n = 795) showed lower BDNF levels compared to non-EDs controls (n = 552) (SMD: − 0.49, 95% CI [− 0.89; − 0.08], p-value = 0.01). Moreover, subgroup analysis was conducted based on the specimen (plasma and serum), which revealed no statistically significant difference in the levels of BDNF between the two subgroups (p-value = 0.92). Additionally, meta-regression results revealed that publication year, mean age of the individuals with EDs, NOS scores, and the number of individuals with EDs collectively accounted for 25.99% percent of the existing heterogeneity.

Conclusion
Lower BDNF levels are associated with EDs.
Qualitative study of patient experiences of mental distress during TB investigation and treatment in Zambia
Authors: Mainga T. , Gondwe M. , Mactaggart I. , Stewart R. C. , Shanaube K. , Ayles H. , Bond V. 
Published: July 2022

Abstract
Background
The mental health and TB syndemic is a topic that remains under-researched with a significant gap in acknowledging and recognizing patient experiences, particularly in the sub-Saharan African region. In this qualitative study conducted in Zambia, we aimed to explore the lived mental health experiences of TB patients focusing on their multi-layered drivers of distress, and by so doing highlighting contextual factors that influence mental distress in TB patients in this setting.

Methods
The study draws on qualitative data collected in 2018 as part of the Tuberculosis Reduction through Expanded Antiretroviral Treatment and Screening for active TB trial (TREATS) being conducted in Zambia. The data was collected through in-depth interviews with former TB patients (n = 80) from 8 urban communities participating in the TREATS trial. Thematic analysis was conducted. Additional quantitative exploratory analysis mapping mental distress symptoms on demographic, social, economic and TB characteristics of participants was conducted.

Results
Most participants (76%) shared that they had experienced some form of mental distress during their TB investigation and treatment period. The reported symptoms ranged in severity. Some participants reported mild distress that did not disrupt their daily lives or ability to adhere to their TB medication, while other participants reported more severe symptoms of distress, for example, 15% of participants shared that they had suicidal ideation and thoughts of self-harm during their time on treatment. Mental distress was driven by unique interactions between individual, social and health level factors most of which were inextricably linked to poverty. Mental distress caused by individual level drivers such as TB morbidity often abated once participants started feeling better, however social, economic and health system level drivers of distress persisted during and beyond TB treatment.

Conclusion
The findings illustrate that mental distress during TB is driven by multi-layered and intersecting stresses, with the economic stress of poverty often being the most powerful driver. Measures are urgently needed to support TB patients during the investigation and treatment phase, including increased availability of mental health services, better social security safety nets during TB treatment, and interventions targeting TB, HIV and mental health stigma.
Trial registration ClinicalTrials.gov NCT03739736. Trial registration date: November 14, 2018.
Plant diversity and community analysis of Sele-Nono forest, Southwest Ethiopia: implication for conservation planning
Authors: Kefalew Alemayehu , Soromessa Teshome , Demissew Sebsebe 
Published: July 2022

AbstractBackgroundStudying the floristic diversity of a certain forest is a basic aspect of the design and management of forest vegetation; and consequently this study focused on the plant diversity and community analysis of the Sele-Nono forest. For the current study, plants were sampled from 90 plots using a stratified random sampling technique along the established strata of the study forest. In all the plots, both floristic and environmental data that were relevant to the study were collected following the state of the art. Based on the collected data, the community types, ordination, floristic diversity, and threats to the forest were analyzed using R-package and SPSS software.ResultsCluster analysis produced seven distinct community types which significantly differed among themselves (Cophentic correlation coefficient = 0.785, P < 0.001) of which community types 2 and 6 were relatively poor; whereas communities 1 and 4 were rich in terms of their species richness and diversity. In addition, Canonical Correspondence Analysis (CCA) suggests that a number of environmental factors such as altitude and slope (topographic factor), OM and N (edaphic factors) and disturbance were the main drivers for the current distribution of plant species and disparity in plant community composition in Sele-Nono forest. Moreover, the study revealed high beta diversity ($${\beta }_{w}$$βw>12) of plant species at the landscape level (i.e., throughout the study forest). Deforestation for agricultural land expansion and degradation through selective logging are the main threats to the Sele-Nono forest.ConclusionsThe present study revealed that the Sele-Nono forest is a large and heterogenous forest at the landscape level (150, 325.27 ha;$${\beta }_{w}$$βw>12). Moreover, it is one of the richest and diverse forest ecosystems in terms of plant biodiversity, and it could qualify to be labeled as a keystone ecosystem. However, currently it is exposed to a variety of threats. We recommend the forest to be developed into a biosphere reserve. We also recommend the prioritization of areas belonging to community types 2 and 6 of the forest for any possible conservation actions so as to maximize species richness and diversity of the native plants of the area.
Virtual reality-based training for mental health staff: a novel approach to increase empathy, compassion, and subjective understanding of service user experience
Authors: Riches Simon , Iannelli Hannah , Reynolds Lisa , Fisher Helen L. , Cross Sean , Attoe Chris 
Published: July 2022

Abstract
Background
Mental health service users report that staff empathy is key to developing positive therapeutic relationships but promoting empathy in staff training is challenging. Staff may struggle to maintain their compassion, particularly in challenging settings, and have limited clinical confidence when treating conditions of which they lack subjective understanding. Novel interventions are required to address these needs.

Main body of the text
Virtual reality-based simulation training has been shown to be an effective training modality for healthcare professionals; it has the potential to deliver crucial empathy-building learning for frontline mental health staff due to its capacity to increase staff understanding of service users’ experiences. Virtual reality and simulation technology take interactivity and experiential learning to a level beyond which we have seen in teaching and training before. Subjective understanding is elicited because this is a technology for enhanced experiential learning, which in turn fosters greater empathy and compassion. Increased empathy in the workforce is likely to yield significant benefits for service users. Greater empathy in nursing is linked with reduced restrictive practices and reduced conflict between staff and service users. Restrictive practices, including restraint and seclusion, are widely used in mental health settings within the UK, and are an aspect of mental health nursing that is at odds with the therapeutic role of nursing. Despite these innovative developments, there are challenges ahead. Many nurses feel that complete eradication of restrictive practices is impossible and that barriers include a limitation of resources, communication, management, and lack of education. There is a need to make simulation training economically viable so that it can be upscaled and widely available. Therefore, greater investment and resources are needed to bring this innovative training to the wider workforce to support staff and to realise the benefits for service users.

Short conclusion
Virtual reality-based training has great potential for mental health staff, which could have important consequences in terms of improved staff empathy and reductions in harmful restrictive practices. Further research and funding for such training is necessary so that it can be more widely available.
Core–shell structured gold nanoparticles as carrier for 166Dy/166Ho in vivo generator
Authors: Wang Runze , Ponsard Bernard , Wolterbeek Hubert , Denkova Antonia 
Published: July 2022

Abstract
Background
Radionuclide therapy (RNT) has become a very important treatment modality for cancer nowadays. Comparing with other cancer treatment options, sufficient efficacy could be achieved in RNT with lower toxicity. β− emitters are frequently used in RNT due to the long tissue penetration depth of the β− particles. The dysprosium-166/holmium-166 (166Dy/166Ho) in vivo generator shows great potential for treating large malignancies due to the long half-life time of the mother nuclide 166Dy and the emission of high energy β− from the daughter nuclide 166Ho. However, the internal conversion occurring after β− decay from 166Dy to 166Ho could cause the release of about 72% of 166Ho when 166Dy is bound to conventional chelators. The aim of this study is to develop a nanoparticle based carrier for 166Dy/166Ho in vivo generator such that the loss of the daughter nuclide 166Ho induced by internal conversion is prevented. To achieve this goal, we radiolabelled platinum-gold bimetallic nanoparticles (PtAuNPs) and core–shell structured gold nanoparticles (AuNPs) with 166Dy and studied the retention of both 166Dy and 166Ho under various conditions.

Results
The 166Dy was co-reduced with gold and platinum precursor to form the 166DyAu@AuNPs and 166DyPtAuNPs. The 166Dy radiolabelling efficiency was determined to be 60% and 70% for the two types of nanoparticles respectively. The retention of 166Dy and 166Ho were tested in MiliQ water or 2.5 mM DTPA for a period of 72 h. In both cases, more than 90% of both 166Dy and 166Ho was retained. The results show that the incorporation of 166Dy in AuNPs can prevent the escape of 166Ho released due to internal conversion.

Conclusion
We developed a chelator-free radiolabelling method for 166Dy with good radiolabelling efficiency and very high stability and retention of the daughter nuclide 166Ho. The results from this study indicate that to avoid the loss of the daughter radionuclides by internal conversion, carriers composed of electron-rich materials should be used.
Augmented reality in architecture and construction education: state of the field and opportunities
Authors: Hajirasouli Aso , Banihashemi Saeed 
Published: July 2022

AbstractOver the past decade, the architecture and construction (AC) industries have been evolving from traditional practices into more current, interdisciplinary and technology integrated methods. Complex and intricate digital technologies and mobile computing such as simulation, computational design and immersive technologies, have been exploited for different purposes such as reducing cost and time, improving design and enhancing overall project efficiency. Immersive technologies and augmented reality (AR), in particular, have proven to be extremely beneficial in this field. However, the application and usage of these technologies and devices in higher education teaching and learning environments are yet to be fully explored and still scarce. More importantly, there is still a significant gap in developing pedagogies and teaching methods that embrace the usage of such technologies in the AC curricula. This study, therefore, aims to critically analyse the current state-of-the-art and present the developed and improved AR approaches in teaching and learning methods of AC, addressing the identified gap in the extant literature, while developing transformational frameworks to link the gaps to their future research agenda. The conducted analysis incorporates the critical role of the AR implications on the AC students’ skillsets, pedagogical philosophies in AC curricula, techno-educational aspects and content domains in the design and implementation of AR environments for AC learning. The outcomes of this comprehensive study prepare trainers, instructors, and the future generation of AC workers for the rapid advancements in this industry.
Cotton stomatal closure under varying temperature and vapor pressure deficit, correlation with the hydraulic conductance trait
Authors: WEDEGAERTNER Kurt , SHEKOOFA Avat , PURDOM Sam , WALTERS Kellie , DUNCAN Lori , RAPER Tyson B. 
Published: July 2022

Abstract
Background
Cotton (Gossypium hirsutum L.) is often grown in locations characterized by high atmospheric evaporative demand. It has been hypothesized that plants which resist hydraulic flow under this condition will limit water use and conserve soil water. Therefore, in a series of controlled environment experiments ten cotton cultivars were exposed to two different temperature and vapor pressure deficit (VPD) conditions (i.e., 38 °C, > 3 kPa and 32 °C, 1∼1.5 kPa) as well as a progressive soil drying. Then, individual differences in shoot hydraulic conductance (Kshoot) was measured using a hydraulic conductance flow meter (HCFM). Physiological parameters were reported included leaf area, dry leaf weight, stomatal conductance (gs), and water use efficiency coefficient (WUEk).

Results
Differences were observed in Kshoot among cultivars under the 38 °C, > 3 kPa but not the 32 °C, 1∼1.5 kPa environment. Under the 38 °C, > 3 kPa environment, correlations were found between Kshoot, stomatal conductance (gs), VPD breakpoint, WUEk, total leaf area, dry leaf weight, fraction transpirable soil water (FTSW) threshold, and slope of TR decline after FTSW threshold.

Conclusion
Results show that the ability of some cotton cultivars to restrict water loss under high evaporative demand through early stomatal closure is associated with the cultivars’ Kshoot. The Kshoot is influential in the limitation of TR trait under high temperature and VPD.