bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs scaffolds for efficient neural network reconstruction in canines with traumatic brain injury
Authors: Liu Xiaoyin , Zhang Guijun , Wei Pan , Zhong Lin , Chen Yaxing , Zhang Jianyong , Chen Xuyi , Zhou Liangxue 
Published: June 2022

Abstract
The secretome secreted by stem cells and bioactive material has emerged as a promising therapeutic choice for traumatic brain injury (TBI). We aimed to determine the effect of 3D-printed collagen/chitosan/secretome derived from human umbilical cord blood mesenchymal stem cells scaffolds (3D-CC-ST) on the injured tissue regeneration process. 3D-CC-ST was performed using 3D printing technology at a low temperature (−20°C), and the physical properties and degeneration rate were measured. The utilization of low temperature contributed to a higher cytocompatibility of fabricating porous 3D architectures that provide a homogeneous distribution of cells. Immediately after the establishment of the canine TBI model, 3D-CC-ST and 3D-CC (3D-printed collagen/chitosan scaffolds) were implanted into the cavity of TBI. Following implantation of scaffolds, neurological examination and motor evoked potential detection were performed to analyze locomotor function recovery. Histological and immunofluorescence staining were performed to evaluate neuro-regeneration. The group treated with 3D-CC-ST had good performance of behavior functions. Implanting 3D-CC-ST significantly reduced the cavity area, facilitated the regeneration of nerve fibers and vessel reconstruction, and promoted endogenous neuronal differentiation and synapse formation after TBI. The implantation of 3D-CC-ST also markedly reduced cell apoptosis and regulated the level of systemic inflammatory factors after TBI.
Why do Big Science projects exist? The role of social preferences
Authors: Vincenzi Marco 
Published: June 2022

AbstractRecent work has excluded sociocultural factors among the determinants of Big Science projects. This paper empirically tests the role of four different measures of social preferences, namely altruism, trust, negative reciprocity, and positive reciprocity, in increasing the likelihood of sustaining international cooperation in Big Science projects. Using a novel database of cross-sectional observations from seventy-six countries, this study finds evidence of a positive and statistically significant relationship between negative reciprocity and both time and risk preferences, namely patience and risk-taking. The science policy implication of this study is that a broader theory of clubs can guide meta-organizations in establishing, maintaining, or denying membership in Big Science projects based on the long-term orientation and reputation as a committed cooperator of a country.
Acetabular Reaming Is a Reliable Model to Produce and Characterize Periarticular Heterotopic Ossification of the Hip
Authors: Negri Stefano , Wang Yiyun , Li Zhao , Qin Qizhi , Lee Seungyong , Cherief Masnsen , Xu Jiajia , Hsu Ginny Ching-Yun , Tower Robert Joel , Presson Bradley , Levin Adam , McCarthy Edward , Levi Benjamin , James Aaron W 
Published: June 2022

Abstract
Heterotopic ossification (HO) is a pathologic process characterized by the formation of bone tissue in extraskeletal locations. The hip is a common location of HO, especially as a complication of arthroplasty. Here, we devise a first-of-its-kind mouse model of post-surgical hip HO and validate expected cell sources of HO using several HO progenitor cell reporter lines. To induce HO, an anterolateral surgical approach to the hip was used, followed by disclocation and acetabular reaming. Animals were analyzed with high-resolution roentgenograms and micro-computed tomography, conventional histology, immunohistochemistry, and assessments of fluorescent reporter activity. All the treated animals’ developed periarticular HO with an anatomical distribution similar to human patients after arthroplasty. Heterotopic bone was found in periosteal, inter/intramuscular, and intracapsular locations. Further, the use of either PDGFRα or scleraxis (Scx) reporter mice demonstrated that both cell types gave rise to periarticular HO in this model. In summary, acetabular reaming reproducibly induces periarticular HO in the mouse reproducing human disease, and with defined mesenchymal cellular contributors similar to other experimental HO models. This protocol may be used in the future for further detailing of the cellular and molecular mediators of post-surgical HO, as well as the screening of new therapies.
The Divergence Between Hospital Charges and Reimbursements For Primary 1–2-level Lumbar Fusion Has Increased Over Time
Authors: Bogdan Paulina , Walocha Daniel , Gordon Adam M. , Lam Aaron , Ng Mitchell K. , Saleh Ahmed , Razi Afshin E. 
Published: June 2022

Study Design:
A retrospective cohort study was performed for patients undergoing 1–2-level lumbar fusion (1–2LF) from 2005 to 2014 using an administrative claims database.

Objective:
The objective of this study was to determine changes in: (1) annual charges; (2) annual reimbursement rates; and (3) annual difference (charges minus reimbursements) in patients undergoing 1–2LF.

Summary of Background Data:
With implementation of value-based care in orthopaedics, coupled with the rise in number of patients undergoing 1–2LF, understanding the discordance in hospital charges and reimbursements is needed. The difference in hospital charges to reimbursements specifically for 1–2LF for degenerative disc disease has not been studied.

Materials and Methods:
A Medicare administrative claims database was queried for patients undergoing primary lumbar fusion using ICD-9 procedural code 81.04–81.08. Patients specifically undergoing 1–2LF were filtered from this cohort using ICD-9 procedural code 81.62. The query yielded 547,067 patients who underwent primary 1–2LF. Primary outcomes analyzed included trends in charges, reimbursement rates, and net difference in cost over time and per annual basis. Linear regression evaluated the change in costs over time with a P-value less than 0.05 considered significant.

Results:
From 2005 to 2014, total charges increased from $6,085,838,407 to $19,621,979,956 and total reimbursements increased from $1,677,764,831 to $4,656,702,685 (all P<0.001). Per patient charges increased 92.10% from 2005 to 2014 for patients undergoing primary 1–2LF from $129,992 to $249,697 (P<0.001). Similarly, an increase in reimbursement per patient of 65.35% from $35,836 to $59,258 (P<0.001) was noted. The annual difference in charges to reimbursements increased 102.26% during the study interval from $94,155 to $190,439 (P<0.001).

Conclusions:
Per patient charges and reimbursements both increased over the study period; however, charges increased 30% more than reimbursements. Further breakdown of hospital, surgeon, and anesthesiologist reimbursements for 1–2LF is needed.

Level of Evidence:
Level III.
Hem-o-lok-free and Retzius-sparing robot-assisted laparoscopic radical prostatectomy with retrograde release of the neurovascular bundle
Authors: Wang Yong , Liu Zihao , Huang Hua , Ma Yuan , Niu Yuanjie 
Published: June 2022

Abstract

Background:
Robot-assisted laparoscopic radical prostatectomy (RARP) has gradually become the standard surgical procedure for prostate cancer. This video, http://links.lww.com/CURRUROL/A10 aimed to demonstrate a new and improved surgical approach for prostate cancer that passes through the pouch of Douglas and allows retrograde release of the neurovascular bundle (NVB). The technique is Hem-o-lok-free and Retzius-sparing and is performed without opening the pelvic floor fascia during RARP.

Methods:
A 69-year-old man was diagnosed with prostate cancer (clinical stage T2bN0M0). We followed the process of Retzius-sparing RARP [published by Galfano et al. in 2013] and did some modification. The critical technical points associated with this technique included locating the fascial layer at the prostatic apex and performing complete retrograde release of the NVB along the anatomical structure. We used diathermy to perform precision hemostasis on large vessels, especially those around the basal part of prostate, with relatively less damage. In addition, Hem-o-lok was not used in this procedure.

Results:
Negative surgical margin and stage T2bN0M0 were confirmed. Continence was achieved immediately after cathter removal. One month after surgery, prostate specific antigen was undetectable. Erectile function was preserved 3 months postoperatively.

Conclusions:
Hem-o-lok-free and Retzius-sparing RARP is a feasible surgical approach that combines the benefits of complete retrograde release of the NVB, preserving the nerves and vascular structures around the prostate, protecting the pelvic floor structure, and ensuring rapid recovery of micturition control.
Coronavirus disease 2019 (COVID-19) and priapism: An unexplored association
Authors: Malinga Dominic Mpumelelo , Laher Abdullah E. , McDowall Jared , Adam Ahmed 
Published: June 2022

Abstract

Background:
Coronavirus disease 2019 (COVID-19) has an established impact on multiple organ systems, including the vascular and urogenital systems. Vascular effects may include venous thromboembolic disease, which could theoretically be a precursor to priapism—a urological emergency defined as an abnormal condition of prolonged penile erection lasting >4 hours. To better explore this association, we critically appraised all the published COVID-19 cases associated with priapism.

Materials and methods:
After PROSPERO registration (CRD42021245257), a systematic search of Google Scholar, Scopus, Embase, Web of Science, PubMed, Cumulative Index to Nursing and Allied Health Literature, Global Index Medicus, and Cochrane Database of Systematic Reviews was performed using specific search terms. The following study metadata were extracted: age, requirement for respiratory support, cavernous blood gas findings, management of priapism, and patient outcomes.

Results:
Fifteen single-patient case reports were included in this review. Of these, all of the patients presented with ischemic priapism, 9 patients (60.0%) were >60 years of age, 4 (26.7%) reported more than a single episode of priapism, 11 (73.3%) presented with pneumonia, 8 (53.3%) required mechanical ventilation, D-dimer was elevated in 5 of the 6 (83.3%) patients in whom this was reported, and among the 13 patients in whom mortality was reported, 4 (30.8%) died.

Conclusions:
Early reports suggest a prognostic relationship between COVID-19 and coexisting priapism. However, owing to commonalities in their pathophysiology and the small dataset reported in the literature, the probable association between COVID-19 and priapism is still theoretical. Further research is needed to confirm this association.
Expert Consensus on Diagnosis and Treatment of End-Stage Liver Disease Complicated with Infections
Authors: Ning Qin , Chen Tao , Wang Guiqiang , Xu Dong , Yu Yanyan , Mao Qing , Li Taisheng , Li Lanjuan , Li Jun , Lu Xiaoju , Li Jiabin , Li Zhiwei , Zhang Wenhong , Xiao Yonghong , Meng Qinghua , Mi Yuqiang , Shang Jia , Yu Yunsong , Zhao Yingren , Zhao Caiyan , Zhao Hong , Huang Jianrong , Peng Jie , Tang Hong , Tang Xiaoping , Hu Jinhua , Hu Bijie , Guo Wei , Zheng Bo , Chen Baiyi , Zhang Yuexin , Wei Jia , Sheng Jifang , Chen Zhi , Wang Minggui , Xie Qing , Wang Yuming , Wang Fu-Sheng , Hou Jinlin , Duan Zhongping , Wei Lai , Jia Jidong ,   Chinese Society of Infectious Disease of Chinese Medical Association
Published: June 2022

Abstract
End-stage liver disease (ESLD) is a life-threatening clinical syndrome that markedly increases mortality in patients with infections. In patients with ESLD, infections can induce or aggravate the occurrence of liver decompensation. Consequently, infections are among the most common complications of disease progression. There is a lack of working procedure for early diagnosis and appropriate management for patients with ESLD complicated by infections as well as local and international guidelines or consensus. This consensus assembled up-to-date knowledge and experience across Chinese colleagues, providing data on principles as well as working procedures for the diagnosis and treatment of patients with ESLD complicated by infections.
Examination of Homologies between COVID-19 Vaccines and Common Allergens: The Potential for T Cell-mediated Responses for Allergic Rhinitis and Asthma
Authors: Hartwell Micah , Greiner Benjamin H. , Nicks Savannah 
Published: June 2022

Abstract
As the SARS-CoV-2 virus shares relatively large protein sequences homologous to grass pollens, dust mites, and molds, our objective was to assess the potential overlap between the COVID-19 mRNA vaccines from Pfizer-BioNtech and Moderna and known allergens. We found 7 common allergens with potential for cross-reactivity with the Pfizer vaccine and 19 with the Moderna vaccine, including common grasses, molds, and dust mites. T-cell mediated antigen cross-reactivity between viruses and allergens is a relatively new area of study in clinical immunology; a discipline that may be particularly useful regarding the SARS-CoV-2 virus and the allergic response in humans. These results suggest that vaccination with the Pfizer-BioNtech and Moderna COVID-19 vaccines may contribute to T-cell cross-reactivity with allergens that impact allergic asthma and allergic rhinitis. Further research should assess the clinical implications of COVID-19 vaccination on the severity and symptomatology of the allergic disease, in addition to natural viral infection.
Advances in HIV Eradication Strategies
Authors: Mei Qingmin , Wang Wei , Wu Jianjun , Gao Yong 
Published: June 2022

Abstract
Although the advent of combination antiretroviral therapy can efficiently suppress human immunodeficiency virus (HIV) replication, a complete cure for HIV infection cannot be achieved due to the existence of latent viral reservoirs. In recent years, investigation of HIV cure strategies has become a hot topic in the field. In this article, we review the major barriers to HIV cure, compare the progress and challenges of non-specific and specific latent reversal agents in curing HIV, and discuss possible solutions to the current problems.
MicroRNAs and their signaling pathway in mycosis fungoides
Authors: Sun Zhiyuan , Yao Xiaona , Ding Xing , Li Xun , Tian Xuewen 
Published: June 2022

Abstract

Background:
Oncogenic microRNAs, a kind of stable epigenetic inhibitors, often deregulated in Mycosis fungoides (MF) which affect the skin and tend to transform and spread.

Results:
Previous studies investigating the de-expression of microRNA in MF patients skin biopsies identified that they were not only regulated by signaling pathway, but also regulated other signaling pathway. Furthermore, studies have elucidated the molecular mechanisms of the STAT signaling pathway that can promote a great diversity of miRNA expression via cytokine binding receptors, activating Janus kinase-3 and STAT proteins. But some non-STAT signaling pathway with mircoRNA de-expression in MF was incomplete.

Conclusion:
Taken together, these studies demonstrate that microRNA may be used as the prognosis, progression and diagnose of MF, as they can not only control MF cell proliferation, but also induce MF cell apoptosis.
Analyzing adverse drug reaction using statistical and machine learning methods
Authors: Kim Hae Reong , Sung MinDong , Park Ji Ae , Jeong Kyeongseob , Kim Ho Heon , Lee Suehyun , Park Yu Rang 
Published: June 2022

Abstract

Background:
Adverse drug reactions (ADRs) are unintended negative drug-induced responses. Determining the association between drugs and ADRs is crucial, and several methods have been proposed to demonstrate this association. This systematic review aimed to examine the analytical tools by considering original articles that utilized statistical and machine learning methods for detecting ADRs.

Methods:
A systematic literature review was conducted based on articles published between 2015 and 2020. The keywords used were statistical, machine learning, and deep learning methods for detecting ADR signals. The study was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement (PRISMA) guidelines.

Results:
We reviewed 72 articles, of which 51 and 21 addressed statistical and machine learning methods, respectively. Electronic medical record (EMR) data were exclusively analyzed using the regression method. For FDA Adverse Event Reporting System (FAERS) data, components of the disproportionality method were preferable. DrugBank was the most used database for machine learning. Other methods accounted for the highest and supervised methods accounted for the second highest.

Conclusions:
Using the 72 main articles, this review provides guidelines on which databases are frequently utilized and which analysis methods can be connected. For statistical analysis, >90% of the cases were analyzed by disproportionate or regression analysis with each spontaneous reporting system (SRS) data or electronic medical record (EMR) data; for machine learning research, however, there was a strong tendency to analyze various data combinations. Only half of the DrugBank database was occupied, and the k-nearest neighbor method accounted for the greatest proportion.
Proposal of a novel protocol using estimated cardiac index fractional dose to improve aortic contrast enhancement for early-phase dynamic CT
Authors: Kuba Tadashi , Tokushige Akihiro , Murayama Sadayuki , Ueda Shinichiro 
Published: June 2022

Abstract
Maximum aortic computed tomography value (CTV) is difficult to control because of variations in cardiac function and patient physique. Therefore, to improve early-phase aortic enhancement on dynamic computed tomography (CT), we developed an estimated cardiac index fractional dose (eciFD). The eciFD protocol is a novel and original protocol for administering fractional dose (FD), representing the amount of iodine per unit body weight per injection duration, based on cardiac index (cardiac output divided by body surface area) as estimated by age in early-phase dynamic CT. At the time of administration, by selecting FD based on the patient's age and selecting a parameter that can achieve this FD, an aortic CTV ≥300 HU (ACTV≥300) can be obtained. This study aimed to investigate aortic enhancement on CT angiography using the eciFD protocol.
This retrospective study investigated 291 consecutive patients who underwent dynamic CT from neck to abdomen after recommendation of the eciFD protocol at our institution. We compared early-phase aortic CTV distributions by scan delay between an eciFD group (eciFD applied, n = 135) and a non-eciFD group (eciFD not applied, n = 80). The effect of eciFD on early-phase ACTV≥300 was evaluated using logistic regression analysis adjusted for several potentially meaningful clinical confounders related to aortic CTV, namely male sex, heart rate ≤80 beats/min, estimated glomerular filtration rate ≤40 mL/min, use of eciFD, bolus tracking (BT), history of myocardial infarction, and order from the emergency center.
The eciFD protocol was a significant factor for early-phase ACTV≥300 after adjusting for several confounders (odds ratio 3.03; 95% confidence intervals 1.59–5.77; P = .001). No interaction was seen between BT and eciFD protocol (p for interaction = 0.76). In terms of CTV distribution, with both a fixed scan delay time and BT, the eciFD group showed a high aortic CTV. The combination of eciFD protocol with BT provided a particularly high percentage of patients with ACTV≥300 (86.4%).
The eciFD protocol was useful for improving aortic contrast enhancement. These findings need to be validated in a randomized controlled study.
Contribution of interleukin 27 serum level to pathogenesis and prognosis in children with immune thrombocytopenia
Authors: Hassan Tamer , Abdel Rahman Doaa , Raafat Nermin , Fathy Manar , Shehab Mohamed , Hosny Ahmed , Fawzy Reham , Zakaria Marwa 
Published: June 2022

Abstract
Concepts surrounding the mechanisms of thrombocytopenia in ITP have shifted from the traditional view of autoantibody mediated platelet destruction to more complex mechanisms in which impaired platelet production, T-cell-mediated effects, and disturbed cytokine profiles play a role. Interleukin 27 (IL-27) plays pleiotropic roles in immunomodulation and autoimmune diseases.
We aimed to determine the level of IL-27 in patients with ITP and its relationship to patient and disease characteristics as well as disease chronicity and response to treatment.
Sixty childrens with primary immune thrombocytopenia were consequetively enrolled in this study as well as 20 age and sex matched healthy controls.
ITP patients had significantly higher levels of IL-27 than controls (770.6 and 373.8 pg/ml, respectively). Patients with acute ITP had the highest levels of IL-27 among patient groups, while patients in remission had the lowest IL-27 levels (860.1and 622.9 pg/ml, respectively). Patients who received IVIG and combined steroids plus IVIG had significantly higher IL-27 levels than others. Patients who received Eltrombopag had significantly lower IL-27 levels than others.
IL-27 seems to play a role in pathogenesis of childhood ITP. IL-27 can be used as a predictor for disease occurrence as well as responsiveness to treatment.
Initial preclinical evaluation of 68Ga-DOTA-(Ser)3-LTVSPWY peptide as a PET radiotracer for glioblastoma targeting and imaging
Authors: Ranjbar Venousheh , Molavipordanjani Sajjad , Biabani Ardakani Javad , Akhlaghi Mehdi , Nikkholgh Babak , Hosseinimehr Seyed Jalal 
Published: June 2022

Purpose
Imaging of glioblastoma multiform (GBM) tumor using 68-Galium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraaceticacid-Ser-Ser-Ser-Leu-Thr-Val-Ser-Pro-Trp-Tyr (68Ga-DOTA-(Ser)3-LTVSPWY) as a PET radiotracer for HER2 receptor due to fact that this receptor plays a pivotal role in the tumorigenesis and tumor progression in a wide range of cancer.

Methods

68Ga-DOTA-(Ser)3-LTVSPWY was produced with high radiochemical purity. The affinity and specificity of this radiotracer toward HER2 receptor on the surface of glioma glioblastoma (U-87 MG) cell line were evaluated. Furthermore, the biodistribution and PET imaging of this radiolabeled peptide were investigated on xenografted U-87 MG tumor-bearing mice.

Results
The in-vitro specific binding study revealed that the 68Ga-DOTA-(Ser)3-LTVSPWY binds to different cell lines with respect to their level of HER2 expression. The calculated KD and Bmax of radiolabeled peptide toward U-87 MG cell line were 5.5 ± 2.4 nmol/l and (2.4 ± 0.3) × 105 receptors per cell, respectively. The highest tumor uptake was observed at 30-min postinjection, whereas the tumor-to-muscle ratio was about four-fold. The acquired PET images distinctively show tumor site, which was blocked with excess nonlabeled peptide that revealed specific in-vivo targeting of 68Ga-DOTA-(Ser)3-LTVSPWY for glioma.

Conclusion

68Ga-DOTA-(Ser)3-LTVSPWY specifically recognizes HER2 receptors and could be a potential candidate for GBM imaging.