bluuebunny/crossref_metadata_2025_split huggingface dataset:
From Null to Midline: Changes in Head Posture do Not Predictably Change Head Tremor in Cervical Dystonia
Authors: Vu Jeanne P. , Cisneros Elizabeth , Zhao Jerry , Lee Ha Yeon , Jankovic Joseph , Factor Stewart A. , Goetz Christopher G. , Barbano Richard L. , Perlmutter Joel S. , Jinnah Hyder A. , Pirio Richardson Sarah , Stebbins Glenn T. , Elble Rodger J. , Comella Cynthia L. , Peterson David A. 
Published: September 2022

Introduction: A common view is that head tremor (HT) in cervical dystonia (CD) decreases when the head assumes an unopposed dystonic posture and increases when the head is held at midline. However, this has not been examined with objective measures in a large, multicenter cohort.Methods: For 80 participants with CD and HT, we analyzed videos from examination segments in which participants were instructed to 1) let their head drift to its most comfortable position (null point) and then 2) hold their head straight at midline. We used our previously developed Computational Motor Objective Rater (CMOR) to quantify changes in severity, amplitude, and frequency between the two postures.Results: Although up to 9% of participants had exacerbated HT in midline, across the whole cohort, paired t-tests reveal no significant changes in overall severity (t = −0.23, p = 0.81), amplitude (t = −0.80, p = 0.43), and frequency (t = 1.48, p = 0.14) between the two postures.Conclusion: When instructed to first let their head drift to its null point and then to hold their head straight at midline, most patient’s changes in HT were below the thresholds one would expect from the sensitivity of clinical rating scales. Counter to common clinical impression, CMOR objectively showed that HT does not consistently increase at midline posture in comparison to the null posture.
Elevated ApoB/ApoA-I ratio is associated with acute anti-N-Methyl-D-aspartate receptor encephalitis, but not disease outcomes
Authors: Liu Yingying , Ma Xiaomeng , Ma Lili , Su Zhumin , Li Donghong , Chen Xiaohong 
Published: September 2022

ObjectiveThe purpose of the present study is to clarify the relationship between the apolipoprotein B100/apolipoprotein A-I (ApoB/ApoA-I) ratio and anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis.MethodsA total of 71 patients with anti-NMDAR encephalitis were included in this study, and their ApoB/ApoA-I ratios in baseline and follow-up were retrospectively analyzed.ResultsThe ApoB/ApoA-I ratio was closely correlated with the baseline-modified Rankin scale (mRS) score of &gt;3 in patients with anti-NMDAR encephalitis. A subgroup analysis showed obvious differences between the high and low ApoB/ApoA-I ratio groups. The ApoB/ApoA-I ratio was positively correlated with intensive care unit (ICU) treatment, length of hospital stay, baseline mRS score, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR). The ratios of the high and low ApoB/ApoA-I groups both improved in the follow-up.ConclusionThe increased ApoB/ApoA-I ratio is associated with acute anti-NMDAR encephalitis, but not disease outcomes. Serum ApoB/ApoA-I ratio was related to inflammation and immunity in peripheral blood. The findings might provide a new idea for further exploration of the pathogenesis and treatment of anti-NMDAR encephalitis.
Global, regional, and national neck pain burden in the general population, 1990–2019: An analysis of the global burden of disease study 2019
Authors: Shin Dong Woo , Shin Jae Il , Koyanagi Ai , Jacob Louis , Smith Lee , Lee Heajung , Chang Yoonkyung , Song Tae-Jin 
Published: September 2022

BackgroundThis study describes the global epidemiology and trends associated with neck pain. Global Burden of Disease data collected between 1990 and 2019 were used to determine the global burden of neck pain in the general populations of 204 countries.MethodsGlobal, regional, and national burdens of neck pain determined by prevalence, incidence, and years lived with a disability (YLD) from 1990 to 2019 were comprehensively analyzed according to age, gender, and socio-demographic index using the Global Burden of Disease Study 1990 and 2019 data provided by the Institute for Health Metrics and Evaluation.ResultsGlobally, in 2019, the age-standardized rates for prevalence, incidence, and YLD of neck pain per 100,000 population was 2,696.5 (95% uncertainty interval [UI], 2,177.0 to 3,375.2), 579.1 (95% UI, 457.9 to 729.6), and 267.4 (95% UI, 175.5 to 383.5) per 100,000 population, respectively. Overall, there was no significant difference in prevalence, incidence, or YLD of neck pain between 1990 and 2019. The highest age-standardized YLD of neck pain per 100,000 population in 2019 was observed in high-income North America (479.1, 95% UI 323.0 to 677.6), Southeast Asia (416.1, 95% UI 273.7 to 596.5), and East Asia (356.4, 95% UI 233.2 to 513.2). High-income North America (17.0, 95% UI 9.0 to 25.4%) had the largest increases in YLD of neck pain per 100,000 population from 1990 to 2019. At the national level, the highest age-standardized YLD of neck pain was found in the Philippines (530.1, 95% UI 350.6 to 764.8) and the highest change age-standardized YLD between 1990 and 2019 was found in the United States (18.4, 95% UI 9.9 to 27.6%). Overall, the global burden of neck pain increased with age until the age of 70–74 years, and was higher in women than men. In general, positive associations between socio-demographic index and burden of neck pain were found.ConclusionsBecause neck pain is a major public health burden with a high prevalence, incidence, and YLD worldwide, it is important to update its epidemiological data and trends to cope with the future burden of neck pain.
Deciphering the combination mechanisms of Gualou–Xiebai herb pair against atherosclerosis by network pharmacology and HPLC-Q-TOF-MS technology
Authors: Liu Yarong , Zhong Hua , Xu Pengbo , Zhou An , Ding Lidan , Qiu Jingwen , Wu Hongfei , Dai Min 
Published: September 2022

Introduction: Gualou (Trichosanthes kirilowii Maxim)–Xiebai (Allium macrostemon Bunge) (GLXB) is a well-known herb pair against atherosclerosis (AS). However, the combination mechanisms of GLXB herb pair against AS remain unclear.Objective: To compare the difference in efficacy between GLXB herb pair and the single herbs and to explore the combination mechanisms of GLXB against AS in terms of compounds, targets, and signaling pathways.Methods: The combined effects of GLXB were evaluated in AS mice. The main compounds of GLXB were identified via quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) and UNIFI informatics platforms. The united mechanisms of GLXB in terms of nodes, key interactions, and functional clusters were realized by network pharmacology. At last, the anti-atherosclerotic mechanisms of GLXB were validated using enzyme-linked immunosorbent assay (ELISA) and Western blot in AS mice.Results: The anti-atherosclerotic effects of the GLXB herb pair (6 g/kg) were more significant than those of Gualou (4 g/kg) and Xiebai (2 g/kg) alone. From the GLXB herb pair, 48 main components were identified. In addition, the GLXB herb pair handled more anti-atherosclerotic targets and more signaling pathways than Gualou or Xiebai alone, whereas 10 key targets of GLXB were found using topological analysis. Furthermore, the GLXB herb pair (6 g/kg) could suppress the inflammatory target levels of IL-6, IL-1β, TNF-α, ALOX5, PTGS2, and p-p38 in AS mice. GLXB herb pair (6 g/kg) could also ameliorate endothelial growth and function by regulating the levels of VEGFA, eNOS, p-AKT, VCAM-1, and ICAM-1 and reducing macrophage adhesion to vascular wall in AS mice. GLXB herb pair (6 g/kg) could improve the blood lipid levels in AS mice. In addition, the regulating effects of GLXB herb pair (6 g/kg) on levels of IL-1β, TNF-α, ALOX5, VEGFA, eNOS, VCAM-1, ICAM-1, and blood lipids were more significant than those of Gualou (4 g/kg) or Xiebai alone (2 g/kg).Conclusion: The combination mechanisms of the GLXB herb pair were elucidated in terms of components, targets, and signaling pathways, which may be related to suppressing inflammation, regulating vascular endothelial growth/function, and improving blood lipid levels.
Empagliflozin induces apoptotic-signaling pathway in embryonic vasculature: In vivo and in silico approaches via chick’s yolk sac membrane model
Authors: Mosallanejad Saeedeh , Mahmoodi Mehdi , Tavakkoli Hadi , Khosravi Ahmad , Salarkia Ehsan , Keyhani Alireza , Dabiri Shahriar , Gozashti Mohammad Hossein , Pardakhty Abbas , Khodabandehloo Hadi , Pourghadamyari Hossein 
Published: September 2022

The present investigation was conducted to evaluate the vascular-toxicity of empagliflozin (EMP) in embryonic vasculature. Firstly, the vascular-toxicity of the drug as well as its interaction with apoptotic regulator proteins was predicted via in silico approach. In the next step, the apoptotic-signaling pathway in embryonic vasculature was evaluated using a chick’s YSM model. In silico simulation confirmed vascular-toxicity of EMP. There was also an accurate affinity between EMP, Bax and Bcl-2 (−7.9 kcal/mol). Molecular dynamics assay revealed complex stability in the human body conditions. Furthermore, EMP is suggested to alter Bcl-2 more than BAX. Morphometric quantification of the vessels showed that the apoptotic activity of EMP in embryonic vasculature was related to a marked reduction in vessel area, vessel diameter and mean capillary area. Based on the qPCR and immunohistochemistry assays, enhanced expression level of BAX and reduced expression level of Bcl-2 confirmed apoptotic responses in the vessels of the YSM. We observed that induction of an apoptotic signal can cause the embryonic defect of the vascular system following EMP treatment. The acquired data also raised suspicions that alteration in apoptotic genes and proteins in the vasculature are two critical pathways in vascular-toxicity of EMP.
Beluga (D. leucas), harbor porpoise (P. phocoena), and killer whale (O. orca) acoustic presence in kotzebue sound, alaska: Silence speaks volumes
Authors: Castellote Manuel , Small Robert J. , Stafford Kathleen M. , Whiting Alex , Frost Kathryn J. 
Published: September 2022

Prior to 1984, belugas (Delphinapterus leucas) were seen in large numbers during spring and summer in Kotzebue Sound, Alaska, and provided an important subsistence resource to coastal residents. Sightings and harvest declined sharply beginning in 1984: the average annual harvest dropped from 84/yr (1977–1983) to 16/yr (1984–2021). To examine the current seasonal and spatial occurrence of belugas in Kotzebue Sound, passive acoustic moorings were deployed in summer 2013 and year-round in 2014–2016. Three moorings were deployed off Cape Krusenstern, northwestern Kotzebue Sound, to monitor cetaceans traveling nearshore. A mooring was also deployed near Chamisso Island, southeastern Kotzebue Sound. We used automatic detectors to process the recordings for echolocation and tonal signals, and all detections were manually validated. Belugas, harbor porpoises (Phocoena), and transient killer whales (Orcinus orca) were detected in both areas, primarily from June to November. Detections extended into early winter for belugas, and sporadic detections were confirmed for porpoises from January to March. Belugas were detected on a total of 20 days, killer whales on 96 days, and porpoises on 179 days. All beluga detections were echolocation signals; the absence of social signals likely reflects an anti-predator response to transient killer whales and possibly to subsistence hunters. Killer whale detections were composed of echolocation signals, limited to very short click trains, double clicks, and single clicks, a known cryptic acoustic behavior used when targeting prey. Killer whales also emitted high frequency whistles (17–51 kHz) providing the first evidence of these types of signals for transients. Our results suggest transient killer whales in predation mode scouting harbor porpoise and beluga habitat, concurrent with belugas in silent anti-predation mode. This anti-predation acoustic behavior by belugas was also evident when killer whales were not present, conveying a continued perception of predation risk for this habitat. The combined natural and anthropogenic predation pressure in Kotzebue Sound could be playing an important role in the continued low occurrence of belugas.
Effects of remnant preservation in anterior cruciate ligament reconstruction: A systematic review and meta-analysis
Authors: Xie Huanyu , Fu Zicai , Zhong Mingjin , Deng Zhenhan , Wang Chen , Sun Yijia , Zhu Weimin 
Published: September 2022

BackgroundCompared with standard anterior cruciate ligament (ACL) reconstruction, it is controversial whether anterior cruciate ligament reconstruction (ACLR) with remnant preservation can lead to better clinical outcomes. We conducted a systematic study and meta-analysis to assess the differences in clinical efficacy between the two.MethodWe searched for clinical randomized controlled studies and cohort studies included in the Cochrane library, PubMed, and Embase from March 2012 to March 2022 in English. The included studies were ACLR with or without remant preservation, and the data were extracted and the quality of the included studies was assessed by two authors, respectively. Revman 5.4 was used for statistical analysis and conclusions were presented.ResultTen articles containing a total of 777 patients were finally included. There was no significant difference in postoperative Lachman test [OR = 1.66, 95%CI (0.79, 3.49), P = 0.18 &gt; 0.05], Tegner score [SMD = −0.13, 95%CI (−0.47, 0.22), P = 0.46 &gt; 0.05], synovial coverage rate by second-look arthroscopy [OR = 1.55, 95%CI (0.66, 3.65), P = 0.32 &gt; 0.05], the rate of cyclops lesion [OR = 3.92, 95%CI (0.53, 29.29), P = 0.18 &gt; 0.05], joint range of motion [SMD = 0.27, 95%CI (−0.13, 0.68), P = 0.19 &gt; 0.05] and re-injury rate [OR = 0.57, 95%CI (0.18, 1.74), P = 0.32 &gt; 0.05] between the two groups. There were statistically significant differences in postoperative Lysholm score [SMD = 0.98, 95% CI (0.32, 1.64), P = 0.004 &lt; 0.05], International Knee Documantation Committee grade (IKDC grade) [OR = 2.19, 95%CI (1.03, 4.65), P = 0.04 &lt; 0.05], Pivot shift test [OR = 1.71, 95%CI (1.06, 2.77), P = 0.03 &lt; 0.05], KT1000/2000 arthrometer side-to-side difference [SMD = −0.22, 95%CI (−0.42, −0.03), P = 0.02 &lt; 0.05], operation time [SMD = 11.69, 95%CI (8.85, 14.54), P = 0.00001 &lt; 0.05] and degree of tibial tunnel enlargement [SMD = −0.66, 95%CI (−1.08, −0.23), P = 0.002 &lt; 0.05].ConclusionThis meta-analysis concluded that remnant preservation significantly had better results in terms of patient functional score (Lysholm, IKDC), knee stability (Pivot shift test, postoperative side-to-side anterior laxity) and tibial tunnel enlargement. In terms of complications (incidence of Cyclops lesions, range of motion, re-injury rate), no significant differences were seen between the two groups. Although many studies concluded that remnant preservation could bring better synovial coverage, this meta-analysis indicated that there is insufficient evidence to support it, possibly due to different remnant preservation procedures.The potential risks associated with longer operation times are also worth considering.
Programmed cell death 1 inhibitor plus chemotherapy vs. chemotherapy in advanced drive-gene-negative non-small-cell lung cancer patients: A real-world study
Authors: Li Ying , Yang Peng , Zhou Xiao , Yang Xuefeng , Wu Shijie 
Published: September 2022

ObjectiveProgrammed cell death 1 (PD-1) inhibitor has been in the market in China for several years, which lacks sufficient domestic evidence regarding its application in lung cancer. Thus, this study intended to assess the treatment outcome and tolerance of PD-1 inhibitor plus chemotherapy in advanced, driver-gene-negative, nonsquamous, non-small-cell lung cancer (NSCLC) patients in a real clinical setting.MethodsThis retrospective cohort study analyzed 68 advanced driver-gene-negative nonsquamous NSCLC patients, among which 38 cases received PD-1 inhibitor plus chemotherapy and 30 cases adopted chemotherapy alone. Disease control rate (DCR), objective response rate (ORR), progression-free survival (PFS), overall survival (OS), and adverse events were reviewed.ResultsGenerally, PD-1 inhibitor plus chemotherapy achieved a more satisfying ORR compared with chemotherapy alone (52.6% vs. 30.0%, P = 0.061), while the DCR did not vary between PD-1 inhibitor plus chemotherapy and chemotherapy (84.2% vs. 73.3%, P = 0.271). Patients receiving PD-1 inhibitor plus chemotherapy exhibited favorable PFS (median: 10.1 vs. 7.1 months, P = 0.040) and OS (median: 17.4 vs. 13.9 months, P = 0.049) than patients adopting chemotherapy alone. Additionally, after adjustment using multivariable Cox's analyses, PD-1 inhibitor plus chemotherapy (vs. chemotherapy) could independently realize prolonged PFS (P = 0.020) and OS (P = 0.029). Moreover, the majority of adverse events were manageable; meanwhile, grade 3–4 adverse events included leukopenia (13.2%), neutropenia (13.2%), nausea and vomiting (7.9%), anemia (5.3%), elevated transaminase (5.3%), thrombopenia (2.6%), anorexia (2.6%), peripheral neuropathy (2.6%), and rash (2.6%).ConclusionPD-1 inhibitor plus chemotherapy exhibits a better efficacy and equal tolerance compared with chemotherapy alone in advanced driver-gene-negative nonsquamous NSCLC patients.
Biopreservation of Chocolate Mousse with Lactobacillus helveticus 2/20: Microbial Challenge Test
Authors: Goranov Bogdan , Teneva Desislava , Denkova-Kostova Rositsa , Shopska Vesela , Oulahal Nadia , Denkova Zapryana , Kostov Georgi , Degraeve Pascal , Pagan Rafael 
Published: September 2022

Probiotic bacteria are used for food biopreservation because their metabolic products might contribute to ensuring food microbiological safety and/or increase its shelf life without the addition of chemical preservatives. Moreover, biopreserved foods are excellent vehicles for the delivery of probiotic bacteria. The aim of the study was to investigate the potential of chocolate mousse food matrix for the delivery of the probiotic strain Lactobacillus helveticus 2/20 (Lb. helveticus 2/20) and to investigate its capacity to inhibit the growth of two foodborne pathogenic bacteria (Staphylococcus aureus and Escherichia coli). Therefore, the populations of free or encapsulated in calcium alginate Lb. helveticus 2/20 cells and/or of each pathogen (used to voluntarily contaminate each sample) were monitored both in complex nutrient medium (MRS broth) and in chocolate mousse under refrigeration conditions and at room temperature. Lb. helveticus 2/20 alone in free or encapsulated state effectively inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 in chocolate mousse when stored at 20 ± 2 °C. Practically no viable unwanted bacteria were identified on the 7th day from the beginning of the process. High viable Lb. helveticus 2/20 cell populations were maintained during storage under refrigerated conditions (4 ± 2 °C) and at room temperature. Chocolate mousse is thus a promising food matrix to deliver probiotic Lb. helveticus 2/20 cells, which could also protect it from contamination by unwanted bacteria.
COVID-19: sequelas pós-infecção
Authors: Da Silva Roseane Maria Ferreira , Melo Natassy Fragoso 
Published: September 2022

Introdução: A pandemia de COVID-19 pegou a todos de surpresa, tornando-se um problema de saúde pública em poucos meses, os internamentos por tal patologia foram de números alarmantes, adoecendo a população que teve que lidar com os sintomas durante a infecção e com sequelas após a cura. Objetivo: Descrever as sequelas pós infecção por COVID-19 e quais os prejuízos para a volta a rotina das pessoas acometidas. Metodologia: trata-se de um estudo de revisão integrativa da literatura que tem como caráter quantitativo e qualitativo. Resultados e discussão: Pessoas acometidas pelo COVID-19 mesmo após alta hospitalar ou exames para detecção do vírus negativo, sentem que a vida não voltou a ser a mesma, as sequelas deixadas por essa patologia são visíveis, dentre elas podemos citar: Fadiga, cansaço, fraqueza, mal-estar. Falta de ar (ou dificuldade para respirar, respiração curta) Fibrose nos pulmões e/ou rins. Perda de paladar e olfato (temporária ou duradoura). Conclusão: O COVID-19 causou uma devasta destruição desde o inicio da pandemia até os dias atuais, milhares de mortes e caos no sistema de saúde, porém várias pessoas através de tratamentos com respostas positivas poderão vencer o vírus, mesmo notando que as sequelas eram presentes na sua vida atual, mediante acompanhamento com especialistas espera-se que aos poucos os sintomas das sequelas regridam e as pessoas consigam viver com qualidade de vida.
Tratamiento de las fracturas supracondíleas de húmero en la Infancia
Authors: Romero Michelle María Loor , Govea Michael Alfredo Cobeña 
Published: September 2022

En el presente estudio se aborda el tratamiento de las fracturas supracondíleas de húmero en la infancia y la terapia más adecuada teniendo en cuenta que estas pueden ser de resolución quirúrgica y no quirúrgica, dependiendo del grado de lesión. En la definición, las fracturas supracondíleas de húmero se precisan como la falta de continuidad de la porción más distal del húmero en la parte superior de los cóndilos cercanos y proximales a la línea fisaria. El objetivo de este artículo de revisión es analizar la evidencia bibliográfica disponible sobre el manejo terapéutico de las fracturas supracondíleas de húmero en la infancia. La obtención de información se realizó a partir de la búsqueda en bases de datos de Ciencias de la Salud: Elsevier, Scielo, Pubmed, Dialnet, MedLine, además de otras revistas médicas, de enfermería y repositorios bibliotecarios universitarios en Google Academic. Se seleccionaron documentos nacionales e internacionales con resumen y disposición completa del texto gratuito, publicados en los últimos 5 años (entre el 2018 -2022), en inglés y español. A través de una matriz bibliográfica se abordan 20 estudios en donde se estudia la temática de esta investigación. Dentro de los principales resultados obtenidos, se observa que hubo un predominio mayor en el sexo masculino; siendo la causa de lesión una hiperextensión de la articulación del codo; producido por caídas en el hogar. En cuanto a la terapéutica, se observa que la mejor forma de establecer un tratamiento adecuado en este tipo de fracturas se relaciona con la clasificación de la escala de Gartland, la cual valora el desplazamiento de la fractura y los resultados desde la parte clínica y radiológica. Basado en esto se concluye que la intervención quirúrgica sigue siendo en la mayoría de los estudios el mejor plan de tratamiento en este tipo de patologías.