bluuebunny/crossref_metadata_2025_split huggingface dataset:
RHD exon 5, 7 and 10 targeted non-invasive prenatal screening of fetal Rhesus-D (RhD) in selected RhD negative pregnant women in Ethiopia
Authors: Niguse Birhanu , Ermias Mihertab , Berhanu Solomon , Abayneh Lemma , Chakiso Bekele , Rather Riyaz Ahmad 
Published: March 2022

Background
A majority of non-invasive prenatal screening studies determining fetal RhD status have been tested on Caucasian and Asian populations, but limited or no studies have been conducted on the Ethiopian population. In the current study, we carried non-invasive prenatal screening of fetal RHD genotype in selected RhD negative Ethiopian pregnant women.

Methods
Cell-free DNA was extracted from the plasma samples of 117 RhD pregnant women between 9 and 38 weeks of gestation. Fetal RHD genotypes were detected by targeting exons 5, 7 and 10 of the RHD gene by using real-time PCR assay. RHD genotypic results were confirmed by neonatal cord blood serology.

Results
Fetal RHD genotyping was conclusive in all 117 subjects. RHD genotype was correctly predicted in 115 of 117 cases, thus the test yielded 98.3% accuracy (95%CI: 97.3–99.1%). Among 115 cases, 105 were genotyped as RHD positive and 12 were genotyped as RHD negative. The sensitivity and specificity of the test were 99.1% (95% CI: 94.8–99.9%) and 91.7% (95%CI: 61.5–99.7%) respectively. The negative and positive predictive values were 99.9% (95%CI: 99.2–99.9%) and 54.0% (95% CI: 15.2–88.4%) respectively. SRY genotyping results were in complete concordance with fetal sex.

Conclusion
Multi exon targeted non-invasive prenatal screening test for fetal RhD determination exhibited high accuracy and sensitivity. A confirmatory study with a bigger size of study subjects is warranted before enabling clinical implementation.
Association between osteoporosis and periodontal disease among menopausal women: The 2013–2015 Korea National Health and Nutrition Examination Survey
Authors: Lee Yunhee 
Published: March 2022

BackgroundThis cross-sectional study aimed to investigate the association between osteoporosis and periodontal disease among Korean menopausal women, as well as the association between osteoporosis and periodontal disease according to duration after menopause.MethodsOf a total of 22,948 subjects who participated in the Korea National Health and Nutrition Examination Survey, from 2013 to 2015 the final study population was limited to 2,573 subjects with no missing values. The subjects were divided into two groups, normal bone mineral density (BMD) and osteoporosis, according to the T-score obtained from bone densitometry. Scores of ≥ 3 points for the community periodontal index of treatment needs were reclassified as periodontal disease. Moreover, after stratification of the variable ‘duration after menopause’ into 0–4, 5–9, and ≥10 years, binary logistic regression analysis was performed to investigate the association between osteoporosis and periodontal disease according to the duration after menopause.ResultsThere was an association between osteoporosis and periodontal disease. The osteoporosis group had an adjusted odds ratio [OR] of 1.25 (95% confidence interval [CI]: 1.00–1.56) for periodontal disease compared to the normal BMD group. Of note, the osteoporosis group in the menopausal transition stage (0–4 years after menopause) showed an adjusted OR of 2.08 (95% CI: 1.15–3.77) for developing periodontal disease.ConclusionsOsteoporosis was associated with periodontal disease and the association was strongest among women in the menopausal transition stage, 0–4 years after menopause. Oral health promotion, including regular oral examination and oral hygiene care, is particularly useful for menopausal transition women with osteoporosis.
Higher Quality Colonoscopy: Worth the Wait?
Authors: Gawron Andrew J. , Dominitz Jason A. 
Published: March 2022

AbstractTo meet the high demand for colonoscopy, the Veterans Health Administration often refers veterans to community practices. Petros et al. compared colonoscopy quality at a Veterans Affairs Medical Center with that of local community practices. Although community providers performed more timely colonoscopy than the Veterans Affairs medical center (mean 25 days earlier), colonoscopy quality was significantly lower for other important quality metrics, including adenoma detection, advanced adenoma detection, adenomas per colonoscopy, and appropriate surveillance recommendations. This study highlights the need for continued efforts to assure high-quality colonoscopy in all settings.
Enrichment of Motilin Receptor Loss-of-Function Variants in Gastroparesis
Authors: Smieszek Sandra P. , Carlin Jesse L. , Xiao Changfu , Birznieks Gunther , Polymeropoulos Christos M. , Polymeropoulos Mihael H. 
Published: March 2022

Introduction:
Gastroparesis is a serious medical condition characterized by delayed gastric emptying and symptoms of nausea, vomiting, bloating, fullness after meals, and abdominal pain.

Methods:
To ascertain the genetic risk factors for gastroparesis, we conducted the largest thus far whole-genome sequencing study of gastroparesis. We investigated the frequency and effect of rare loss-of-function variants in patients with both idiopathic and diabetic gastroparesis enrolled in a clinical study of gastroparesis.

Results:
Among rare loss-of-function variants, we reported an increased frequency of a frameshift mutation p.Leu202ArgfsTer105, within the motilin receptor gene, variant rs562138828 (odds ratio 4.9). We currently replicated this finding in an independent large cohort of gastroparesis samples obtained from patients participating in the ongoing phase III gastroparesis clinical study.

Discussion:
Motilin receptor is an important therapeutic target for the treatment of hypomotility disorders. The identified genetic variants may be important risk factors for disease as well as may inform treatments, especially those targeting motilin receptor.
Comparative Safety, Efficacy and Survival Outcome of Anti-PD-1 Immunotherapy in Colorectal Cancer Patients With vs Without Hepatitis B Virus Infection: A Multicenter Cohort Study
Authors: Cheng Yi-Kan , Chen Ping , Chen Dong-Wen , Lin Zhen-sen , Ye Shu-Biao , Lan Ping 
Published: March 2022

INTRODUCTION:
Antiprogrammed cell death protein-1 (PD-1) immunotherapy has substantially broadened in scope for the treatment of colorectal cancer (CRC). However, comparative safety, efficacy and survival outcome of anti-PD-1 therapy in CRC patients with and without hepatitis B virus (HBV) infection remain unclear.

METHODS:
This multicenter, retrospective cohort study included 180 advanced-stage CRC patients with available serological markers for HBV infection treated with anti-PD-1 therapy at the Sixth Affiliated Hospital, Sun Yat-sen University and Sun Yat-sen University Cancer Center between December 2016 and December 2019. A propensity score-matched analysis was performed to compare the safety, efficacy, and survival outcome between HBV and non-HBV groups.

RESULTS:
The incidences of deficient mismatch repair and metastatic disease were significantly different between HBV and non-HBV groups (both P < 0.05). After propensity score-matched analysis, any grade immune-related adverse events and grade ≥ 3 immune-related adverse events were 47% vs 38% (P = 0.25) and 5% vs 6% (P = 1.0) between HBV and non-HBV groups, respectively. The overall response rate was 39% with 17 complete responses and 13 partial responses for the HBV infection cohort and 39% with 11 complete responses and 19 partial responses for the non-HBV infection cohort (P = 1.0). Two-year progression-free survival rates were 38% vs 40% (P = 0.596) and 2-year overall survival rates were 55% vs 63% (P = 0.401) for HBV vs non-HBV infection cohorts.

DISCUSSION:
The incidences of toxicity, efficacy and survival outcome were similar between patients with HBV infection and non-HBV patients receiving anti-PD-1 therapy, which supports to include CRC patients with HBV in clinical trials of anti-PD-1 therapy.
Pemetaan Jalur Jalan Wisata Bukit Jamur di Kabupaten Bengkayang Menggunakan Sistem Informasi Geografis (SIG)
Authors: Yuniarti Erni , Fitriani Meta Indah , Khairiyah Tita 
Published: March 2022

Bukit Jamur is located in a strategic area near Bengkayang Urban and is an urban tourism icon that is popular with visitors to do light-scale hiking. Although located near urban areas, there are no information boards so visitors are not aware of the existence of tourist sites and the lack of road directions both in the village and on the hiking trails. In addition, inadequate road infrastructure makes visitors feel uncomfortable. The delivery of information with a map is expected to make it easier for visitors to know the initial condition of the tourist location. The purpose of this research is to map the road to tourist sites equipped with road signs and information boards so that visitors can know the route clearly. The analysis method in this research uses the method of implementing Geographic Information System (SIG). The findings in the field stated that there are 2 village road paths, namely the Belangko Hamlet road and the Dusun Jaku Bawah road path that is still lacking road directions and there are 3 hiking paths, namely stairways cliff paths and weeds that have not been equipped with directions and information boards so that there needs to be additional directions and information boards in the village and climbing.
A new EPS beads strengthening technology and its influences on axial compressive properties of concrete
Authors: Ji Yuan , Linbing Wang , Weilong Li , Hailu Yang , Jianjun Wang , Wenhua Zhang , Zhenzhen Xiong 
Published: March 2022

Abstract
In this article, a new technology of surface wrapping and strengthening of expandable polystyrene (EPS) beads is proposed. Three wrapping materials including magnesium phosphate cement (MPC), ultra-high performance concrete (UHPC), and water-borne polyurethane (WPU) were evaluated. Four groups of uniaxial compression tests of EPS concrete specimens were conducted: the failure process and the characteristics of the specimens were analyzed; and the complete stress–strain equation is proposed based on these analyses. The research shows that the EPS beads were strengthened by wrapping, and the axial compressive strength of concrete is increased by 50–75%; the concrete with EPS beads wrapped by MPC and WPU has obvious brittleness; the concrete with EPS beads wrapped by UHPC has axial compressive strength close to that of EPS concrete wrapped with MPC and WPU but has a larger ductility coefficient (150%), a larger toughness coefficient (50–90% larger), a higher energy dissipation coefficient (40% larger), and larger residual stress. The proposed stress–strain model is in good agreement with the experimental results, which can accurately describe the stress and deformation characteristics of EPS concrete under uniaxial compression and has good applicability. The discovery of this study provides background knowledge for the structural analysis and design of this kind of concrete; it provides a basis for selecting EPS concrete as structural materials.
Large variation in timing of follow-up visits after hip replacement: a review of the literature
Authors: Loppini Mattia , Gambaro Francesco Manlio , Nelissen Rob G H H , Grappiolo Guido 
Published: March 2022

The study investigated the existing guidelines on the quality and frequency of the follow-up visits after total hip replacement surgery and assessed the level of evidence of these recommendations.
The review process was carried out according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Additional works were retrieved by direct investigation of the available guidelines of the most important orthopedic societies and regulatory agencies.
The current systematic review of the literature resulted in zero original papers, four guidelines for routine follow-up and three guidelines for special cases. Concerning the quality of evidence behind them, these guidelines were not evidence based but drafted from expert consensus.
The most important finding of this review is the large variation of recommendations in the follow-up schedule after total hip arthroplasty and the lack of evidence-based indications. Indeed, all the above-reported guidelines are the result of a consensus among experts in the field (level of recommendation class D ‘very low’) and not based on clinical studies.
Editorial
Authors: Moreno Ospina Manuel Ignacio 
Published: March 2022

La pandemia mundial de COVID-19 ha sumido al mundo en una crisis sin precedentes. En muchos países la enfermedad ha llevado al límite la capacidad de los proveedores de servicios de salud y las restricciones impuestas por los Gobiernos han perturbado considerablemente la economía mundial.
La Oficina de las Naciones Unidas Contra la Droga y el Delito (UNODC) ha alertado la complejidad del consumo de SPA en contexto de epidemia. Este escenario ha supuesto un importante impacto en la vida diaria de las personas y un reto para los servicios que prestan atención a las personas con adicción. Esta situación ha podido condicionar cambios en las pautas de consumo de sustancias psicoactivas y otras adicciones. Es probable que se aumenten los riesgos vinculados al tráfico de drogas y al abuso de las mismas, teniendo en cuenta que, como afirmó Waly Gahada, directora ejecutiva de la UNODC, “nuestros sistemas sociales y sanitarios están al borde del abismo y nuestras sociedades penan para hacer frente” (2020).
Editorial
Authors: Lozano Juan Ignacio 
Published: March 2022

En el presente Dossier temático propusimos abordar el debate sobre política de drogas en Latinoamérica desde un punto de vista interdisciplinario y crítico. La convocatoria se dirigió a producciones que promuevan debates en torno a aportes históricos, jurídicos, geopolíticos, sanitarios y sociológicos, con el objeto de relevar experiencias, investigaciones o ensayos acerca de los debates actuales a nivel local, regional e internacional. Intuimos que, al referirnos a experiencias y procesos, hacíamos relevancia a abordajes que desarrollaran reducción de daños, despenalización del consumo, regulación de sustancias, usos medicinales, indultos a personas procesadas o encarceladas, como así también a las consecuencias de políticas punitivistas que derivan en militarización, en escenarios de tráfico a gran escala, presencia de crimen organizado, corrupción, lavado de activos, encarcelamientos masivos, desproporción penal, temas que trascienden lógicas locales, y que tienen impacto en los derechos humanos.
SOCIOBIODIVERSIDADE E CONSERVAÇÃO DO CERRADO EM SÃO RAIMUNDO DAS MANGABEIRAS, MARANHÃO
Authors: Arouche-Lima Ione Marly , Costa Filho José Da Luz , De Sousa Antônia Pereira , Lima Júnior Lacídio Pereira 
Published: March 2022

A falta de créditos que contempla a produção familiar agroextrativista, a escassez de assistência técnica especializada e o impacto negativo gerado pela monocultura de grãos vêm provocando problemas ambientais, econômicos e sociais que são destacados na área de estudo deste trabalho. Portanto, objetivou-se identificar e refletir sobre a organização das comunidades Bacuri, Assentamento Nova Descoberta e Descanso, no estado do Maranhão, sobre o modo como os saberes locais são aplicados ao uso e manejo dos recursos naturais, nesse caso, no bioma cerrado. Buscou-se refletir sobre os aspectos socioeconômicos e a imbricada relação com a Sociobiodiversidade e saberes locais para a conservação ambiental. Ademais, analisou-se como esses saberes podem dialogar com os saberes acadêmicos na busca de uma estratégia viável de conservação das espécies do cerrado.Palavras-chaveAgricultura Familiar. Conservação. Produção.
​Pathogenicity of Root Knot Nematode (Meloidogyne incognita) and its Effect on Yield of Cowpea [Vigna unguiculata (L.) Walp]
Authors: Thakur D. , Pradhan P. , Sahoo J.P. , Subudhi R.P. , Bastia R. , Pradhan J. , Das M.P. 
Published: March 2022

Background: Cowpea [Vigna unguiculata (L.) Walp.] is an important food legume in the drier region and an essential component of the tropics and subtropics for the cropping system. Heavy infestation of cowpea by root knot nematode Meloidogyne incognita led to early senescence of the crop. The experiment was carried out to determine the potential level and the extent of yield loss in cowpea due to root knot nematode. Methods: A pot culture study and a field experiment were conducted to determine pathogenic potential level of root knot nematode and to determine the yield loss in cowpea with various treatment of 10, 100, 1000 and 10000 freshly hatched juveniles. Result: The treatment with nematicide, Carbofuran 3G @ 3 kg a.i./ha, reduced the final nematode population i.e., 50.94% in soil, 46.12% on root and 46.51% decrease in root knot index. The present study revealed that 1000 J2/pot was the ETL level that reduced all the growth parameters of cowpea and avoidable yield loss of 15.78% was recorded in Ccowpea due to M. incognita.
Weed Dynamics and Productivity of French Bean (Phaseolus vulgaris L.) as Influenced by Organic Sources of Nutrients and Weed Management
Authors: Sharma Ashu , Kachroo Dileep , Puniya R. , Stanzen Lobzang 
Published: March 2022

Background: Balancing productivity, profitability and environmental health is a key challenge for maintaining agricultural sustainability. The use of locally available agro-inputs in agriculture by avoiding or minimizing the use of synthetically compounded agro-chemicals appears to be one of the probable options to sustain the agricultural productivity. Widespread use of herbicides has resulted in purported environmental and health problems as well as residual toxicity issues in succeeding crops. At present, the awareness on safe food is increasing. So, keeping this point in view the present investigation was carried out to evaluate the influence of organic sources of nutrients and weed management on growth, yield and weed flora of Frenchbean. Methods: A field experiment was conducted during summer season of 2016 and 2017. The present investigation was laid out in split plot design with six sources of nutrients in main plot and four weed management treatments in sub plots. Result: Application of treatment T5 recorded significantly higher growth parameters, yield attributes, fresh pod yield, net returns and B:C ratio of French bean which was statistically at par with T3 and T2. Amongst the weed management treatments, treatment W1 resulted in significantly lowest species wise and total weed density and biomass, highest weed control efficiency, lowest weed index, highest growth, yield attributes and fresh pod yield of French bean which was statistically at par with W2 and W0. However, the highest net returns and B:C ratio were obtained in weed free plots.
Chinese localisation of wine aroma descriptors: an update of <i> Le Nez du Vin </i> terminology by survey, descriptive analysis and similarity test
Authors: Jin Gang , Lv Xi , Wei Linsheng , Xu Laichao , Zhang Junxiang , Chen Yanping , MA Wen 
Published: March 2022

Aroma is an important criterion for describing and evaluating wine.
The aroma descriptor system currently used in wine analysis is based on Western culture and is thus often inappropriate for use by other cultures to enhance their enjoyment of wine. The objective of the present study was to update this widely applied system to facilitate Chinese participation. The 54 wine aroma descriptors of the original Le Nez du Vin Master Kit were updated based on the results of a survey of 150 untrained Chinese wine consumers who identified the aromas unfamiliar to them. A descriptive sensory analysis was then performed by a panel of 22 untrained wine tasters to identify hypothesised equivalents of the remote lexicons. New aroma reference standards were proposed to verify the hypothesised equivalents between local and non-local terms based on the similarity test. Three unfamiliar descriptors were kept owing to their similarity to the revised attributes and no suitable substitutions were found for four aromas. In the new system, 13 terms are replaced by Chinese local aroma attributes. To the best of our knowledge, this study is the first to propose a Chinese-localised wine aroma wheel consisting of 7 categories, 16 sub-categories and 53 descriptors.
Modelling survival and Factors associated with HIV infected and uninfected patients of Prostate cancer at the University Teaching Hospital Lusaka Zambia
Authors: Mwangilwa Kelvin , Mwale Moses , Msadabwe Susan Citonje , Vinikool Michael , Musonda Patrick 
Published: March 2022

Abstract
Background
Prostate cancer continues to have a high incidence and fatality rate over the world, especially among those infected with the Human Immunodeficiency Virus (HIV), with higher mortality in Africa than on other continents. Zambia has one of the highest anticipated prostate cancer death rates, despite modest progress and a little higher than normal prevalence of prostate cancer. As a result, this study examined the survival rates of HIV-infected and HIV-uninfected prostate cancer patients, as well as the variables that influence mortality.
Methods
We used data from the cancer disease hospital (CDH) in Lusaka, Zambia, to perform a 5-year retrospective cohort analysis of prostate cancer registry data. The data was analysed using a Weibull hazard model at multivariate analysis after doing multiple imputation to deal with missing information in the data. Patients were followed up using mobile phone calls to estimate the contribution of their patients' time from the moment of diagnosis to death or the conclusion of the research period. Those who were lost to follow-up were censored at the date of their last known hospital follow-up.
Results
662 cases were evaluated. The total amount of time at risk was 7548 months. 290 (43.8%) deaths suggest a death rate of 430 people per 10,000 people per year. The average length of survival was 16 months. In an adjusted model, the following variables had a statistically significant impact on the hazard of death: one year increase in age, increased the hazard of death by about 3%, AHR 1.03 (95% CI: 1.02, 1.05, p= 0.001); HIV positive had reduced hazard of death by about 41%, AHR 0.59 ( 95% CI: 0.44, 0.79, p= 0.001); Gleason scoreless or equal to eight, the patients with Gleason score greater than eight had increased hazard of death by about 43%, AHR 1.43 (95% CI: 1.27, 1.59, p= 0.001); those on hormonal therapy had reduced hazard of death by about 28% AHR 0.72 (95% CI: 0.54, 0.94, p= 0.018); and those presented with tumour stage one and two had reduced hazard of death by about 82%, AHR 0.18 (95% CI: 0.04, 0.78), p= 0.021).
Conclusion
Survival from death after learning whether a patient was HIV positive or negative was higher in HIV positives than in HIV negatives, which could be related to HIV negatives having higher Gleason scores than HIV positives who are regularly seen by clinicians for screening. Prostate cancer screening must be advocated for, especially among HIV negative patients, as evidenced by the increased hazard of death compared to HIV positives.