bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-world effectiveness of anti-interleukin-23 antibodies in chronic plaque-type psoriasis of patients from the Austrian Psoriasis Registry (PsoRA)
Authors: Graier T. , Weger W. , Jonak C. , Sator P. , Zikeli C. , Prillinger K. , Sassmann C. , Gruber B. , Saxinger W. , Ratzinger G. , Painsi C. , Mlynek A. , Häring N. , Sadoghi B. , Trattner H. , Müllegger R. , Quehenberger F. , Salmhofer W. , Wolf Peter 
Published: September 2022

AbstractWith the introduction of the latest class of biologic drugs targeting interleukin (IL)-23p19, three new, highly effective drugs can be used for the treatment of chronic plaque psoriasis. However, poorer skin improvement as well as higher rates of serious adverse events have been reported for patients under real-world conditions (outside clinical trials). This accounts especially for patients who have already been treated with biologic drugs. We therefore aimed to determine effectiveness and safety of IL-23p19 inhibitors in real-world patients by analysing data from the Psoriasis Registry Austria (PsoRA) in this observational, retrospective, multicentre cohort study. Data for 197 patients (52.3% biologic-non-naïve), who were treated with anti-IL-23p19 antibodies (127 guselkumab, 55 risankizumab and 15 tildrakizumab) for at least 3 months, were eligible for analysis. In general, biologic-non-naïve patients displayed a less favourable response to anti-IL-23 treatment as compared to biologic-naïve patients. However, after correction for previous biologic exposure, few differences in PASI improvement were detected among biologic-naïve and -non-naïve patients treated with different IL-23p19 inhibitors. This indicates that treatment effectiveness is not related to the class of the previously administered therapy in biologic-non-naïve patients. Therefore, IL-23p19 inhibitors represent a promising treatment alternative for patients who have not responded to previous biologics. However, as with other biologic agents (including IL-17 inhibitors), we did not observe an entirely satisfactory treatment response (i.e. PASI < 3 and/or PASI 75) to anti-IL-23 treatment in one out of four to five patients. Adverse events (mainly non-severe infections) were observed in 23 (11.7%) patients with no major differences regarding the administered IL-23 inhibitor or previous biologic exposure.
Gender specific somatic symptom burden and mortality risk in the general population
Authors: Atasoy Seryan , Hausteiner-Wiehle Constanze , Sattel Heribert , Johar Hamimatunnisa , Roenneberg Casper , Peters Annette , Ladwig Karl-Heinz , Henningsen Peter 
Published: September 2022

AbstractGender specific all-cause mortality risk associated with ahighsomatic symptom burden (SSB) in a population-based cohort was investigated. The study population included 5679 women and 5861 men aged 25–74 years from the population-based MONICA/KORA Cohort. SSB was assessed following the Somatic Symptom Scale-8 and categorized asvery high(≥ 95th percentile),high(60–95th percentile),moderate(30–60th percentile), andlow(≤ 30th percentile). The impact of SSB on all-cause mortality risk within a mean follow-up period of 22.6 years (SD 7.1; 267,278 person years) was estimated by gender-specific Cox regression models adjusted for sociodemographic, lifestyle, somatic and psychosocial risk factors, as well as pre-existing medical conditions. Approximately 5.7% of men and 7.3% of women hadvery highSSB. During follow-up, 3638 (30.6%) mortality cases were observed. Men with avery-highSSB had 48% increased relative risk of mortality in comparison to men with alowSSB after adjustment for concurrent risk factors (1.48, 95% CI 1.20–1.81,p< .0001), corresponding to 2% increased risk of mortality for each 1-point increment in SSB (1.02; 95% CI 1.01–1.03;p= 0.03). In contrast, women with avery highSSB had a 22% lower risk of mortality (0.78, 95% CI 0.61–1.00,p= 0.05) and women withhighSSB had an 18% lower risk of mortality (0.82; 95% CI 0.68–0.98,p= 0.03) following adjustment for concurrent risk factors. The current findings indicate that an increasing SSB is an independent risk factor for mortality in men but not in women, pointing in the direction of critical gender differences in the management of SSB, including women’s earlier health care utilization than men.
Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice
Authors: Li Chenhao , Gao Qingyuan , Jiang Hao , Liu Chengrun , Du Yujun , Li Lisha 
Published: September 2022

AbstractImmune cells play an important role in the development of inflammation in type 1 diabetes mellitus, so we want to explore the changes of CD4+T cells and macrophages in vivo, which can provide an experimental basis for immunotherapy based on CD4+T cells and macrophages. The intraperitoneal injection of streptozocin was used to induce a type 1 diabetes mellitus mouse model; the blood glucose, body weight, and the expression of inflammatory factors in the kidney were measured. Immunohistochemistry was applied to determine and analyze the infiltration of CD4+T cells and macrophages in the spleen, pancreas, and kidney. The subtypes of macrophages in the kidney and CD4+T cells in the spleen were analyzed by flow cytometry. Our study suggests that CD4+T cells and macrophages increase, while the inflammatory immune response system is activated in the development of T1DM. CD4+T cells positively correlated with macrophages in the pancreas and kidney of T1DM. CD4+T cells turn to pro-inflammatory subtypes in the spleen of T1DM, while macrophages turn to pro-inflammatory subtypes in the kidney of T1DM. Therefore, regulation of CD4+T cells and macrophages may be a potential target for T1DM and kidney complications.
Distinct histone H3 modification profiles correlate with aggressive characteristics of salivary gland neoplasms
Authors: Lam-Ubol Aroonwan , Phattarataratip Ekarat 
Published: September 2022

AbstractPost-translational modification of histones is the crucial event that affect many tumor-specific traits. A diverse type of histone modifications had been reported in different cancers with prognostic implications. This study aimed to examine the degree of histone H3 modifications in salivary gland neoplasms and their associations with tumor pathologic characteristics and proliferative activity. The expression of H3K9Ac, H3K18Ac, H3K9Me3 and Ki-67 in 70 specimens of salivary gland neoplasms, consisting of 30 mucoepidermoid carcinoma (MEC), 20 adenoid cystic carcinoma (ACC) and 20 pleomorphic adenoma (PA), were investigated immunohistochemically. The immunohistochemical scoring of 3 histone modification types and Ki-67 labeling index were determined. Overall, MEC demonstrated elevated H3K9Ac level compared with benign PA. Increased H3K9Me3 in MEC was positively correlated with small nest invasion at tumor front, advanced pathologic grade, and elevated proliferative index. In addition, the significant upregulation of all 3 types of histone H3 modification was noted in solid subtype of ACC and associated with increased cell proliferation. This study indicates that salivary gland neoplasms differentially acquire distinct patterns of histone H3 modification, which impact prognostically relevant cancer phenotypes. The hyperacetylation and methylation of histone H3 could be underpinning the prognostically worsen solid type of ACC, and the trimethylation of H3K9 may be involved in aggressive characteristics of MEC.
Compellingly high SARS-CoV-2 susceptibility of Golden Syrian hamsters suggests multiple zoonotic infections of pet hamsters during the COVID-19 pandemic
Authors: Blaurock Claudia , Breithaupt Angele , Weber Saskia , Wylezich Claudia , Keller Markus , Mohl Björn-Patrick , Görlich Dirk , Groschup Martin H. , Sadeghi Balal , Höper Dirk , Mettenleiter Thomas C. , Balkema-Buschmann Anne 
Published: September 2022

AbstractGolden Syrian hamsters (Mesocricetus auratus) are used as a research model for severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Millions of Golden Syrian hamsters are also kept as pets in close contact to humans. To determine the minimum infective dose (MID) for assessing the zoonotic transmission risk, and to define the optimal infection dose for experimental studies, we orotracheally inoculated hamsters with SARS-CoV-2 doses from 1 * 105 to 1 * 10−4 tissue culture infectious dose 50 (TCID50). Body weight and virus shedding were monitored daily. 1 * 10−3 TCID50 was defined as the MID, and this was still sufficient to induce virus shedding at levels up to 102.75 TCID50/ml, equaling the estimated MID for humans. Virological and histological data revealed 1 * 102 TCID50 as the optimal dose for experimental infections. This compelling high susceptibility leading to productive infections in Golden Syrian hamsters must be considered as a potential source of SARS-CoV-2 infection for humans that come into close contact with pet hamsters.
Loneliness and diurnal cortisol levels during COVID-19 lockdown: the roles of living situation, relationship status and relationship quality
Authors: Hopf Dora , Schneider Ekaterina , Aguilar-Raab Corina , Scheele Dirk , Morr Mitjan , Klein Thomas , Ditzen Beate , Eckstein Monika 
Published: September 2022

AbstractLoneliness and social isolation have become increasing concerns during COVID-19 lockdown through neuroendocrine stress-reactions, physical and mental health problems. We investigated living situation, relationship status and quality as potential moderators for trait and state loneliness and salivary cortisol levels (hormonal stress-responses) in healthy adults during the first lockdown in Germany. N = 1242 participants (mean age = 36.32, 78% female) filled out an online questionnaire on demographics, trait loneliness and relationship quality. Next, N = 247 (mean age = 32.6, 70% female) completed ecological momentary assessment (EMA), collecting twelve saliva samples on 2 days and simultaneously reporting their momentary loneliness levels. Divorced/widowed showed highest trait loneliness, followed by singles and partnerships. The latter displayed lower momentary loneliness and cortisol levels compared to singles. Relationship satisfaction significantly reduced loneliness levels in participants with a partner and those who were living apart from their partner reported loneliness levels similar to singles living alone. Living alone was associated with higher loneliness levels. Hierarchical linear models revealed a significant cross-level interaction between relationship status and momentary loneliness in predicting cortisol. The results imply that widowhood, being single, living alone and low relationship quality represent risk factors for loneliness and having a partner buffers neuroendocrine stress responses during lockdown.
The moderating effect of physical activity on the association between screen-based behaviors and chronic diseases
Authors: Araujo Raphael H. O. , Werneck André O. , Barboza Luciana L. , Silva Ellen C. M. , Silva Danilo R. 
Published: September 2022

AbstractWe analyzed the associations of screen-based behaviors with obesity, hypertension, and diabetes, and the moderation of different physical activity (PA) domains in these associations. We used data from the 2019 Brazilian National Health Survey, including data from 80,940 adults (mean age of 32.6 years). TV viewing, other screens (PC, tablet, and cell phone), PA domains (leisure-time, occupational, and transport) were collected via interview. Logistic regression models were used. There was a dose–response association of higher TV viewing with diabetes. Within the groups with medium and higher time spent on other screens, those with < 150 min/week in leisure-time PA increased the odds for obesity [1–2.9 h/day: OR = 1.18 (1.01, 1.39)] and hypertension [1–2.9 h/day: OR = 1.29 (1.08, 1.53); ≥ 6 h/day: OR = 1.47 (1.03, 2.09)]. Likewise, among the participants who spent ≥ 6 h/day of TV viewing, those with < 150 min/week of occupational PA presented higher odds for hypertension [OR = 1.61 (1.03, 2.53)]. In the group with higher use of other screens, < 150 min per week of occupational PA was associated with lower odds for obesity [1–2.9 h/day: OR = 0.81 (0.68, 0.97)] and hypertension [≥ 6 h/day: OR = 0.65 (0.44, 0.98)]. In conclusion, the associations of other screens with obesity and hypertension were strongest among those without leisure-time PA, while the moderator role of occupational PA was not clear.
1D solitons in cubic-quintic fractional nonlinear Schrödinger model
Authors: Stephanovich V. A. , Olchawa W. , Kirichenko E. V. , Dugaev V. K. 
Published: September 2022

AbstractWe examine the properties of a soliton solution of the fractional Schrö dinger equation with cubic-quintic nonlinearity. Using analytical (variational) and numerical arguments, we have shown that the substitution of the ordinary Laplacian in the Schrödinger equation by its fractional counterpart with Lévy index $$\alpha$$
α
permits to stabilize the soliton texture in the wide range of its parameters (nonlinearity coefficients and $$\alpha$$
α
) values. Our studies of $$\omega (N)$$

ω
(
N
)

dependence ($$\omega$$
ω
is soliton frequency and N its norm) permit to acquire the regions of existence and stability of the fractional soliton solution. For that we use famous Vakhitov-Kolokolov (VK) criterion. The variational results are confirmed by numerical solution of a one-dimensional cubic-quintic nonlinear Schrödinger equation. Direct numerical simulations of the linear stability problem of soliton texture gives the same soliton stability boundary as within variational method. Thus we confirm that simple variational approach combined with VK criterion gives reliable information about soliton structure and stability in our model. Our results may be relevant to both optical solitons and Bose-Einstein condensates in cold atomic gases.
Analysis of flow rate and pressure in syringe-based wound irrigation using Bernoulli's equation
Authors: Lee Hanna , Lee Ye-kyung , Park Ji-Yun , Han Jeong-won 
Published: September 2022

AbstractThe objective of this study was to examine the dependence of the pressure level in the wound area on the height of the syringe needle from the wound, the gauge of the needle, and the flow rate using the Bernoulli equation. This study was the control-volume analysis using the Bernoulli equation. At a given height of the syringe needle from the wound, the gauge of the syringe needle was fixed, and the pressure in the wound area, which depended on the flow rate of the irrigation solution discharged from the tip of the needle, was calculated according to the Bernoulli equation and the definition of the flow rate. At a constant flow rate of the irrigation solution, the velocity of the irrigation solution discharged through the syringe needle decreased (7.80 → 0.80) with an increase in the diameter of the needle (18G → 14G). At a constant inner diameter of the needle, the velocity of the irrigation solution increased with a reduction in the flow rate of the solution. As the velocity of the irrigation solution increased, the pressure in the wound area increased. As the height of the syringe needle from the wound area increased, the pressure in the wound area increased. In order to maintain the pressure of 8–15 psi when nurses perform syringe-based irrigation, it is necessary to set the flow rate of the cleaning solution from 3.5 cc/s to less than 4.8 cc/s for 19G. In addition, 20G maintains the flow rate of the solution at 2.6 cc/s or more and less than 3.5 cc/s, 22G maintains the flow rate of solution at 1.3 cc/s or more and less than 1.8 cc/s, and 25G maintains the flow rate of solution at 0.5 cc/s. This study provides nurses with a reference for the flow rate at which syringe-based irrigation can be performed while maintaining the appropriate pressure based on fluid dynamics, which can be used as the basis for wound nursing standards.
Phenol-soluble modulin α and β display divergent roles in mice with staphylococcal septic arthritis
Authors: Hu Zhicheng , Kopparapu Pradeep Kumar , Ebner Patrick , Mohammad Majd , Lind Simon , Jarneborn Anders , Dahlgren Claes , Schultz Michelle , Deshmukh Meghshree , Pullerits Rille , Nega Mulugeta , Nguyen Minh-Thu , Fei Ying , Forsman Huamei , Götz Friedrich , Jin Tao 
Published: September 2022

AbstractPhenol-soluble modulin α (PSMα) is identified as potent virulence factors in Staphylococcus aureus (S. aureus) infections. Very little is known about the role of PSMβ which belongs to the same toxin family. Here we compared the role of PSMs in S. aureus-induced septic arthritis in a murine model using three isogenic S. aureus strains differing in the expression of PSMs (Newman, Δpsmα, and Δpsmβ). The effects of PSMs on neutrophil NADPH-oxidase activity were determined in vitro. We show that the PSMα activates neutrophils via the formyl peptide receptor (FPR) 2 and reduces their NADPH-oxidase activity in response to the phorbol ester PMA. Despite being a poor neutrophil activator, PSMβ has the ability to reduce the neutrophil activating effect of PSMα and to partly reverse the effect of PSMα on the neutrophil response to PMA. Mice infected with S. aureus lacking PSMα had better weight development and lower bacterial burden in the kidneys compared to mice infected with the parental strain, whereas mice infected with bacteria lacking PSMβ strain developed more severe septic arthritis accompanied with higher IL-6 and KC. We conclude that PSMα and PSMβ play distinct roles in septic arthritis: PSMα aggravates systemic infection, whereas PSMβ protects arthritis development.
Clonal dynamics underlying the skewed CD4/CD8 ratio of mouse thymocytes revealed by TCR-independent barcoding
Authors: Iwanami Norimasa , Petersen Malte , Diekhoff Dagmar , Boehm Thomas 
Published: September 2022

AbstractT cell differentiation in the thymus generates CD4+helper and cytotoxic CD8+cells as the two principal T cell lineages. Curiously, at the end of this complex selection process, CD4+cells invariably outnumber CD8+cells. Here, we examine the dynamics of repertoire formation and the emergence of the skewed CD4/CD8 ratio using high-resolution endogenous CRISPR/Cas9 barcoding that indelibly marks immature T cells at the DN2/DN3 pre-TCR stage. In wild-type mice, greater clone size of CD4+cells and an intrinsically greater probability of Tcr β clonotypes for pMHCII interactions are major contributors to the skewed CD4/CD8 ratio. Clonal perturbations of thymocyte differentiation following the precocious expression of a rearranged iNKT invariant TCR α chain are due to loss of Tcr β clonotypes from the CD4 lineage-committed pre-selection repertoire. The present barcoding scheme offers a novel means to examine the clonal dynamics of lymphocyte differentiation orthogonal to that using TCR clonotypes.
CPEB2 m6A methylation regulates blood–tumor barrier permeability by regulating splicing factor SRSF5 stability
Authors: Zhang Mengyang , Yang Chunqing , Ruan Xuelei , Liu Xiaobai , Wang Di , Liu Libo , Shao Lianqi , Wang Ping , Dong Weiwei , Xue Yixue 
Published: September 2022

AbstractThe blood–tumor barrier (BTB) contributes to poor therapeutic efficacy by limiting drug uptake; therefore, elevating BTB permeability is essential for glioma treatment. Here, we prepared astrocyte microvascular endothelial cells (ECs) and glioma microvascular ECs (GECs) as in vitro blood–brain barrier (BBB) and BTB models. Upregulation of METTL3 and IGF2BP3 in GECs increased the stability ofCPEB2mRNA through its m6A methylation. CPEB2 bound to and increasedSRSF5mRNA stability, which promoted the ETS1 exon inclusion. P51-ETS1 promoted the expression of ZO-1, occludin, and claudin-5 transcriptionally, thus regulating BTB permeability. Subsequent in vivo knockdown of these molecules in glioblastoma xenograft mice elevated BTB permeability, promoted doxorubicin penetration, and improved glioma-specific chemotherapeutic effects. These results provide a theoretical and experimental basis for epigenetic regulation of the BTB, as well as insight into comprehensive glioma treatment.
Endogenous oxytocin exerts anti-nociceptive and anti-inflammatory effects in rats
Authors: Nishimura Haruki , Yoshimura Mitsuhiro , Shimizu Makiko , Sanada Kenya , Sonoda Satomi , Nishimura Kazuaki , Baba Kazuhiko , Ikeda Naofumi , Motojima Yasuhito , Maruyama Takashi , Nonaka Yuki , Baba Ryoko , Onaka Tatsushi , Horishita Takafumi , Morimoto Hiroyuki , Yoshida Yasuhiro , Kawasaki Makoto , Sakai Akinori , Muratani Masafumi , Conway-Campbell Becky , Lightman Stafford , Ueta Yoichi 
Published: September 2022

AbstractOxytocin is involved in pain transmission, although the detailed mechanism is not fully understood. Here, we generate a transgenic rat line that expresses human muscarinic acetylcholine receptors (hM3Dq) and mCherry in oxytocin neurons. We report that clozapine-N-oxide (CNO) treatment of our oxytocin-hM3Dq-mCherry rats exclusively activates oxytocin neurons within the supraoptic and paraventricular nuclei, leading to activation of neurons in the locus coeruleus (LC) and dorsal raphe nucleus (DR), and differential gene expression in GABA-ergic neurons in the L5 spinal dorsal horn. Hyperalgesia, which is robustly exacerbated in experimental pain models, is significantly attenuated after CNO injection. The analgesic effects of CNO are ablated by co-treatment with oxytocin receptor antagonist. Endogenous oxytocin also exerts anti-inflammatory effects via activation of the hypothalamus-pituitary-adrenal axis. Moreover, inhibition of mast cell degranulation is found to be involved in the response. Taken together, our results suggest that oxytocin may exert anti-nociceptive and anti-inflammatory effects via both neuronal and humoral pathways.
The impact of protozoa addition on the survivability of Bacillus inoculants and soil microbiome dynamics
Authors: Mawarda Panji Cahya , Le Roux Xavier , Acosta Melissa Uribe , van Elsas Jan Dirk , Salles Joana Falcão 
Published: September 2022

Abstract
Protists’ selective predation of bacterial cells is an important regulator of soil microbiomes, which might influence the success of bacterial releases in soils. For instance, the survival and activity of introduced bacteria can be affected by selective grazing on resident communities or the inoculant, but this remains poorly understood. Here, we investigated the impact of the introduction in the soil of two protozoa species, Rosculus terrestris ECOP02 and/or Cerocomonas lenta ECOP01, on the survival of the inoculants Bacillus mycoides M2E15 (BM) or B. pumilus ECOB02 (BP). We also evaluated the impact of bacterial inoculation with or without protozoan addition on the abundance and diversity of native soil bacterial and protist communities. While the addition of both protozoa decreased the survival of BM, their presence contrarily increased the BP abundance. Protists’ selective predation governs the establishment of these bacterial inoculants via modifying the soil microbiome structure and the total bacterial abundance. In the BP experiment, the presence of the introduced protozoa altered the soil community structures and decreased soil bacterial abundance at the end of the experiment, favouring the invader survival. Meanwhile, the introduced protozoa did not modify the soil community structures in the BM experiment and reduced the BM + Protozoa inoculants’ effect on total soil bacterial abundance. Our study reinforces the view that, provided added protozoa do not feed preferentially on bacterial inoculants, their predatory behaviour can be used to steer the soil microbiome to improve the success of bacterial inoculations by reducing resource competition with the resident soil microbial communities.