bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asian Horntail Eriotremex formosanus (Matsumura) (Insecta: Hymenoptera: Symphyta: Siricidae: Tremicinae)
Authors: Li You , Hulcr Jiri 
Published: October 2022

Since it was introduced to North America, the Asian woodwasp has become the most common wood wasp in Florida. It is not considered an economically important pest because it only attacks dying or dead trees, but the species may someday prove to be a pest and its ecological impacts in North American forests remain unknown. This 4-page fact sheet was written by You Li and Jiri Hulcr, and published by the UF Department of Entomology and Nematology, June 2015. (Photo credit: You Li, UF/IFAS)
EENY 628/IN1095: Asian Horntail Eriotremex formosanus (Matsumura) (Insecta: Hymenoptera: Symphyta: Siricidae: Tremicinae) (ufl.edu)
Spondias Growing in the Florida Home Landscape
Authors: Crane Jonathan H. , Wasielewski Jeff 
Published: October 2022

Spondias species (whose common names among English speakers include ambarella, Ataheite apple, mombins, and hog plums) are flowering trees native to tropical and subtropical regions. They are known for their sweet fruit and grow well in the warmest parts of Florida. This revised 8-page fact sheet discusses biology, distribution and uses, as well as guidelines for propagation and maintenance. Written by Jonathan H. Crane and Jeffrey Wasielewski, and published by the UF Department of Horticultural Sciences, April 2015. (Photo by Forest & Kim Starr, CC BY 3.0)
HS63/MG059: Spondias Growing in the Florida Home Landscape (ufl.edu)
Gastrointestinal Parasites of Farmed White-tailed Deer (Odocoileus virginianus) in Florida
Authors: Cottingham Sydney L. , Walden Heather D.S. , Wisely Samantha M. , Campos-Krauer Juan M. 
Published: October 2022

White-tailed deer are susceptible to infection by numerous gastrointestinal parasites. Not all parasite infections cause clinical disease, but even subclinical gastrointestinal parasitism may reduce the health and productivity of infected deer. An understanding of parasite epidemiology is critical to improving captive deer health through effective parasite control strategies. This publication will serve as a guide to common parasites of farmed white-tailed deer in Florida and will provide producers with recommendations for management of gastrointestinal parasites in farmed deer.
Theoretical and Experimental Gas Volume Quantiﬁcation of Micro- and Nanobubble Ultrasound Contrast Agents
Authors: Abenojar Eric C. , Bederman Ilya , de Leon Al C. , Zhu Jinle , Hadley Judith , Kolios Michael , Exner Agata A. 
Published: October 2022

<p> The amount of gas in ultrasound contrast agents is related to their acoustic activity. Because of this relationship, gas volume has been used as a key variable in normalizing the in vitro and in vivo acoustic behavior of lipid shell-stabilized bubbles with different sizes and shell components. Despite its importance, bubble gas volume has typically only been theoretically calculated based on bubble size and concentration that is typically measured using the Coulter counter for microbubbles and nanoparticle tracking analysis (NTA) for nanoscale bubbles. However, while these methods have been validated for the analysis of liquid or solid particles, their application in bubble analysis has not been rigorously studied. We have previously shown that resonant mass measurement (RMM) may be a better-suited technique for sub-micron bubble analysis, as it can measure both buoyant and non-buoyant particle size and concentration. Here, we provide validation of RMM bubble analysis by using headspace gas chromatography/mass spectrometry (GC/MS) to experimentally measure the gas volume of the bubble samples. This measurement was then used as ground truth to test the accuracy of theoretical gas volume predictions based on RMM, NTA (for nanobubbles), and Coulter counter (for microbubbles) measurements. The results show that the headspace GC/MS gas volume measurements agreed well with the theoretical predictions for the RMM of nanobubbles but not NTA. For nanobubbles, the theoretical gas volume using RMM was 10% lower than the experimental GC/MS measurements; meanwhile, using NTA resulted in an 82% lower predicted gas volume. For microbubbles, the experimental gas volume from the GC/MS measurements was 27% lower compared to RMM and 72% less compared to the Coulter counter results. This study demonstrates that the gas volume of nanobubbles and microbubbles can be reliably measured using headspace GC/MS to validate bubble size measurement techniques. We also conclude that the accuracy of theoretical predictions is highly dependent on proper size and concentration measurements. </p>
PMMA-Fe3O4 for internal mechanical support and magnetic thermal ablation of bone tumors
Authors: Yu Kexiao , Liang Bing , Zheng Yuanyi , Exner Agata , Kolios Michael , Xu Tiantian , Guo Dajing , Cai Xiaojun , Wang Zhigang , Ran Haitao , Chu Lei , Deng Zhongliang 
Published: October 2022

<p>Background: Minimally invasive modalities are of great interest in the field of treating bone tumors. However, providing reliable mechanical support and fast killing of tumor cells to achieve rapid recovery of physical function is still challenging in clinical works. </p>
<p>Methods: A material with two functions, mechanical support and magnetic thermal ablation, was developed from Fe3O4 nanoparticles (NPs) distributed in a polymethylmethacrylate (PMMA) bone cement. The mechanical properties and efficiency of magnetic field-induced thermal ablation were systematically and successfully evaluated in vitro and ex vivo. CT images and pathological examination were successfully applied to evaluate therapeutic efficacy with a rabbit bone tumor model. Biosafety evaluation was performed with a rabbit in vivo, and a cytotoxicity test was performed in vitro. </p>
<p>Results: An NP content of 6% Fe3O4 (PMMA-6% Fe3O4, mFe: 0.01 g) gave the most suitable performance for in vivo study. At the 56-day follow-up after treatment, bone tumors were ablated without obvious side effects. The pathological examination and new bone formation in CT images clearly illustrate that the bone tumors were completely eliminated. Correspondingly, after treatment, the tendency of bone tumors toward metastasis significantly decreased. Moreover, with well-designed mechanical properties, PMMA-6�3O4 implantation endowed tumor-bearing rabbit legs with excellent bio-mimic bone structure and internal support. Biosafety evaluation did not induce an increase or decrease in the immune response, and major functional parameters were all at normal levels. </p>
<p>Conclusion: We have presented a novel, highly efficient and minimally invasive approach for complete bone tumor regression and bone defect repair by magnetic thermal ablation based on PMMA containing Fe3O4 NPs; this approach shows excellent heating ability for rabbit VX2 tibial plateau tumor ablation upon exposure to an alternating magnetic field (AMF) and provides mechanical support for bone repair. The new and powerful dual-function implant is a promising minimally invasive agent for the treatment of bone tumors and has good clinical translation potential.</p>
Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study
Authors: Moore Michael
J. , Sebastian Joseph A. , Kolios Michael 
Published: October 2022

<p>While the nucleus-to-cytoplasmic (N:C) ratio has traditionally been used for assessing cell malignancy, most N:C measurement techniques are time-consuming and performed on thin histological sections, which prohibit assessment of three-dimensional cell structure. A combined ultrahigh frequency ultrasound (US) and photoacoustic (PA) technique was used to assess the size and N:C ratio of cultured cancer cells in three dimensions (3D). The diameters of the cells and their stained nuclei were obtained by fitting the power spectrum of backscattered US pulses and emitted PA waves, respectively, to well-established theoretical models. For comparison, an imaging flow cytometer (IFC) was also used to determine the two-dimensional cell and nucleus sizes from large cell populations using brightfield and fluorescence images, respectively. An N:C ratio was calculated for each cell using the quotient of the measured nucleus diameter and the total cell diameter. The mean N:C ratios calculated using the sound-based approach were 0.68, 0.66, and 0.54 for MCF-7, PC-3, and MDA-MB-231 cells, respectively, and were in good agreement with the corresponding values of 0.68, 0.67, and 0.68 obtained using the IFC. The combined US and PA technique, which assesses cellular N:C ratio in 3D, has potential applications in the detection of circulating tumor cells in liquid biopsies. </p>
Biomechanical evaluation of a novel repair strategy for intervertebral disc herniation in an ovine lumbar spine model
Authors: Page Mitchell I. , Easley Jeremiah T. , Bonilla Andres F. , Patel Vikas V. , Puttlitz Christian M. 
Published: October 2022

Following herniation of the intervertebral disc, there is a need for advanced surgical strategies to protect the diseased tissue from further herniation and to minimize further degeneration. Accordingly, a novel tissue engineered implant for annulus fibrosus (AF) repair was fabricated via three-dimensional fiber deposition and evaluated in a large animal model. Specifically, lumbar spine kinetics were assessed for eight (n = 8) cadaveric ovine lumbar spines in three pure moment loading settings (flexion-extension, lateral bending, and axial rotation) and three clinical conditions (intact, with a defect in the AF, and with the defect treated using the AF repair implant). In ex vivo testing, seven of the fifteen evaluated biomechanical measures were significantly altered by the defect. In each of these cases, the treated spine more closely approximated the intact biomechanics and four of these cases were also significantly different to the defect. The same spinal kinetics were also assessed in a preliminary in vivo study of three (n = 3) ovine lumbar spines 12 weeks post-implantation. Similar to the ex vivo results, functional efficacy of the treatment was demonstrated as compared to the defect model at 12 weeks post-implantation. These promising results motivate a future large animal study cohort which will establish statistical power of these results further elucidate the observed outcomes, and provide a platform for clinical translation of this novel AF repair patch strategy. Ultimately, the developed approach to AF repair holds the potential to maintain the long-term biomechanical function of the spine and prevent symptomatic re-herniation.
New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments
Authors: Spinner Siân A. M. , Barnes Zoe H. , Puinean Alin Mirel , Gray Pam , Dafa’alla Tarig , Phillips Caroline E. , Nascimento de Souza Camila , Frazon Tamires Fonseca , Ercit Kyla , Collado Amandine , Naish Neil , Sulston Edward , Ll. Phillips Gwilym C. , Greene Kelleigh K. , Poletto Mattia , Sperry Benjamin D. , Warner Simon A. , Rose Nathan R. , Frandsen Grey K. , Verza Natalia C. , Gorman Kevin J. , Matzen Kelly J. 
Published: October 2022

For more than 60 years, efforts to develop mating-based mosquito control technologies have largely failed to produce solutions that are both effective and scalable, keeping them out of reach of most governments and communities in disease-impacted regions globally. High pest suppression levels in trials have yet to fully translate into broad and effective Aedes aegypti control solutions. Two primary challenges to date–the need for complex sex-sorting to prevent female releases, and cumbersome processes for rearing and releasing male adult mosquitoes–present significant barriers for existing methods. As the host range of Aedes aegypti continues to advance into new geographies due to increasing globalisation and climate change, traditional chemical-based approaches are under mounting pressure from both more stringent regulatory processes and the ongoing development of insecticide resistance. It is no exaggeration to state that new tools, which are equal parts effective and scalable, are needed now more than ever. This paper describes the development and field evaluation of a new self-sexing strain of Aedes aegypti that has been designed to combine targeted vector suppression, operational simplicity, and cost-effectiveness for use in disease-prone regions. This conditional, self-limiting trait uses the sex-determination gene doublesex linked to the tetracycline-off genetic switch to cause complete female lethality in early larval development. With no female progeny survival, sex sorting is no longer required, eliminating the need for large-scale mosquito production facilities or physical sex-separation. In deployment operations, this translates to the ability to generate multiple generations of suppression for each mosquito released, while being entirely self-limiting. To evaluate these potential benefits, a field trial was carried out in densely-populated urban, dengue-prone neighbourhoods in Brazil, wherein the strain was able to suppress wild mosquito populations by up to 96%, demonstrating the utility of this self-sexing approach for biological vector control. In doing so, it has shown that such strains offer the critical components necessary to make these tools highly accessible, and thus they harbour the potential to transition mating-based approaches to effective and sustainable vector control tools that are within reach of governments and at-risk communities who may have only limited resources.
The relationships between cholesterol crystals, NLRP3 inflammasome, and coronary atherosclerotic plaque vulnerability in acute coronary syndrome: An optical coherence tomography study
Authors: Xue Chao , Chen Qizhi , Bian Ling , Yin Zhaofang , Xu Zuojun , Zhang Huili , Zhang Qingyong , Zhang Junfeng , Wang Changqian , Du Run , Fan Li 
Published: October 2022

BackgroundCholesterol crystals (CCs) in lesions are the hallmark of advanced atherosclerotic plaque. Previous studies have demonstrated that CCs could activate NLRP3 inflammasome, which played an important role in atherosclerotic lesion progression. However, the relationship between CCs, NLRP3 inflammasome pathway, and plaque vulnerability in patients with ACS is still not elucidated.MethodsTwo hundred sixty-nine consecutive acute coronary syndrome (ACS) patients with 269 culprit lesions were included in this study. CCs and other plaque characteristics within the culprit lesion segment were evaluated by optical coherence tomography (OCT) before percutaneous coronary intervention (PCI). The NLRP3 mRNA expression in peripheral blood mononuclear cells (PBMCs) and the serum levels of interleukin (IL)-1β, IL-18, and other biological indices were measured.ResultsCholesterol crystals were observed in 105 (39%) patients with 105 culprit lesions. There were no significant differences in baseline clinical characteristics between the patients with CCs (CCs group, n = 105) and the patients without CCs (non-CCs group, n = 164) within the culprit lesion segment except for lipoprotein(a) [Lp(a)]. The CCs group had a higher level of NLRP3 mRNA expression in PBMCs and higher levels of serum cytokine IL-1β and IL-18. OCT showed that the CCs group had longer lesion length, more severe diameter stenosis, and less minimum luminal area (MLA) than the non-CCs group (all p &lt; 0.05). The frequency of thin-cap fibroatheroma (TCFA), thrombus, accumulation of macrophages, plaque rupture, micro-channel, calcification, spotty calcification, and layered plaque was higher in the CCs group than in the non-CCs groups (all p &lt; 0.05). Multivariate logistic analysis revealed that the level of NLRP3 expression (OR = 10.204), IL-1β levels (OR = 3.523), IL-18 levels (OR = 1.006), TCFA (OR = 3.593), layered plaque (OR = 5.287), MLA (OR = 1.475), macrophage accumulation (OR = 2.881), and micro-channel (OR = 3.185) were independently associated with CCs.ConclusionAcute coronary syndrome patients with CCs in culprit lesions had a higher expression of NLRP3, IL-1β, and IL-18, and had more vulnerable plaque characteristics than patients without CCs. CCs might have interacted with NLRP3 inflammasome activation in patients with ACS, which could contribute to plaque vulnerability in culprit lesions.
Spatial and temporal variation of aseismic creep along the dilational jog of the West Valley Fault, Philippines: Hazard implications
Authors: Rimando Rolly E. , Kurita Katsumi , Kinugasa Yoshihiro 
Published: October 2022

Accelerated creep, primarily through vertical displacement, has been occurring along 15 en-echelon faults belonging to a 15 km-long and 1.5 km-wide, N-S-trending dilational jog of the active West Valley Fault (or West Marikina Valley Fault), on the southeastern part of Metro Manila, Philippines. The much-larger-than-known tectonic slip rates had been the only reliable evidence in support of excessive groundwater withdrawal as the trigger of creep in the 1990s. Recently available groundwater extraction data (1977–2019) could more directly and consistently link groundwater withdrawal to accelerated creep in the 1990s. Twenty years (1999–2019) of precise displacement measurements could also reveal significant spatial and temporal links between slip rate changes and patterns of groundwater extraction. Our analysis shows that greater rates of vertical displacement are related to the timing of faster extraction rates. Variations in slip rates between the northern and southern measurement sites are primarily due to regional differences in groundwater extraction, which are influenced by differences in the implementation of water extraction reduction regulations. Proximity to the key source of groundwater recharge (Laguna Lake) is also an influencing factor. Although there are many unknowns inherent to this type of study, continued depressurization could induce static stress changes that could modulate the timescale of earthquake occurrence due to the natural course of stress loading driven by regional tectonics. The current and potential effects of continued depressurization in the areas with high slip rates and surrounding regions are paramount considerations in crafting and implementing tighter and extended groundwater extraction regulations.
The critical BMI hypothesis for puberty initiation and the gender prevalence difference: Evidence from an epidemiological survey in Beijing, China
Authors: Liu Meijuan , Cao Bingyan , Luo Qipeng , Wang Qiao , Liu Min , Liang Xuejun , Wu Di , Li Wenjing , Su Chang , Chen Jiajia , Gong Chunxiu 
Published: October 2022

BackgroundAlthough previous studies suggested that there is a certain level of body fat mass before puberty can be initiated, most studies have focused on girls.ObjectiveTo investigate the relationship between precocious puberty and physical growth in school-aged children in Beijing, China.Methods7590 Chinese children (3591 girls and 3999 boys) aged 6–11 years were recruited in Beijing, China. Body mass index (BMI) categories were defined by WHO Child Growth Standards and central obesity were defined by sex-specific waist-to-height ratio cut-offs (≥0.46 for girls, ≥0.48 for boys). Sexual development was assessed using Tanner criteria.ResultsThe prevalence of general obesity and central obesity among boys was higher than that in girls. Girls had a significantly higher precocious puberty rate than boys (5.93% vs. 0.87%), particularly in those aged 7 years old (9.20%). Children in the general obesity and central obesity groups have a higher prevalence of precocious puberty and earlier median ages for the attainment of Tanner B2/T2. For girls with Tanner stages≥II at 6-year-old and 7-year-old, the mean BMI was equivalent to the 50th centile of a normal 9.9-year-old and 11.9-year-old girl, respectively. The mean BMI of boys with Tanner stages≥II at 7-year-old and 8-year-old was correspondent to the 50th centile of a normal 14-year-old and 15.3-year-old boy, respectively. For girls, general obesity appears to contribute to the risk of the development of precocious puberty to a greater extent than central obesity does. For boys, central obesity, but not general obesity, was an independent risk factor for precocious puberty.ConclusionsThe prevalence of childhood obesity and precocious puberty was high in China. Precocious puberty was correlated with a large BMI. Boys had a higher threshold of BMI for puberty development than girls. Children with precocious puberty, particularly those with central obesity, should be aware of adverse cardiovascular events.
Immunogenic cell death mediation patterns reveal novel paradigm for characterizing the immune microenvironment and immunotherapeutic responses in bladder cancer
Authors: Fu Jialei , Zhang Wei , Jiang Tao 
Published: October 2022

Background: Immunogenic cell death (ICD) plays an important role in several malignancies. However, the role of ICD-mediated patterns in bladder cancer (BCA) remains unknown.Methods: For assessing the ICD-mediated patterns based on the expression of IRGs, 4 large BCA cohorts were obtained. The ICD-mediated patterns of individual samples were quantified as an ICD score by principal component analysis. The correlations of the ICD-mediated patterns with the tumor immune microenvironment (TIME) and responses to immunotherapy were comprehensively evaluated. The IRGs with predictive prognostic values were further validated by in vitro loss of function assays.Results: Two distinct ICD-mediated patterns were established, showing distinct clinical features and immune microenvironment features. Although ICD cluster A was associated with a poor prognosis with a high ICD score, it showed an immune activation state with a more favorable response to immunotherapy and treatment that induced ICD. The ICD-related gene, CALR, was significantly upregulated in the T24 BCA cell line relative to the control SV-HUC-1 cells. Knocking down CALR suppressed T24 cell viability and caused ER stress.Conclusion: We identified the existence of distinct ICD-mediated patterns in BCA closely associated with the remodeling of the TIME. Further in-depth examination of ICD-related features is warranted to obtain a broader prospect for therapeutic innovations and improved prognosis of BCA.