bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of hypoxia-related genes for prognosis, immune features, and drugs treatment strategy in gastric cancer using bulk and single-cell RNA-sequencing
Authors: Tao Guoqiang , Jiao Chengwen , Wang Yong , Zhou Qi 
Published: December 2022

AbstractHypoxia is one of the malignant characteristics of solid tumors and is related to the multiple malignant characteristics of the tumor. No study has not yet reported a systematical analysis of the characteristics of hypoxia from single-cell resolution in gastric cancer. In our research, we investigated the hypoxia features of various types of cells in single-cell resolution, identified hypoxia-related genes by the weighted gene co-expression network analysis method. Through the hypoxia-related genes from single-cell levels, we screened out 13 genes and established a prognostic model. This model performs well in the training dataset and multiple independent verification data sets. We thought that tumor hypoxia might affect the DNA methylation of cells and promote the transcription of genes associated with malignant features, thereby promoting tumor progression. We found that the more tumor associated genes in the high-risk group showed hypomethylation and high hypoxia-risk score group have more tumor-related genes, more immunosuppressive immune cells and more enrichment of cancer -related pathways. The lower risk group is more sensitive to three chemotherapy drugs for gastric cancer. Our study illustrates the crucial role of hypoxia in gastric cancer. Hypoxia-related gene prognostic model has been established and has good performance. Hypoxia-related risk score can also be used to guide a patient’s drug treatment strategy.
A robust and resilience machine learning for forecasting agri-food production
Authors: Lotfi Reza , Gholamrezaei Amin , Kadłubek Marta , Afshar Mohamad , Ali Sadia Samar , Kheiri Kiana 
Published: December 2022

AbstractThis research proposes a new framework for agri-food capacity production by considering resiliency and robustness and paying attention to disruption and risk for the first time. It is applied robust stochastic optimization by adding robustness to the constraint's objective function and resiliency situation. This research minimizes the mean absolute deviation and coefficient of standard deviation errors by linear function in the agri-food capacity production. This study suggests agri-food managers and decision-makers use this mathematical method to forecast and improve production management. The results of this research lead to better decision-making and are compared with other sine functions. The main model's Robust and Resiliency Mean Absolute Deviation (RRMAD) value is 1.28% lower than other sine-type functions. The conservativity coefficient, confidence level, weight factor, resiliency coefficient, and probability of the scenario vary. The main model's RRMAD value is 1.28% lower than other sine-type functions. Growing the weight factor will result in an increase in RRMAD and a smooth decline inR-squared. Additionally, as the resilience coefficient rises, the RRMAD function increases while theR-squareddeclines. By altering the probability of the scenario, the RRMAD function drops, and theR-squaredgoes up.
COVID-19 plasma exosomes promote proinflammatory immune responses in peripheral blood mononuclear cells
Authors: Chen Lechuang , Chen Rui , Yao Min , Feng Zhimin , Yuan Guoxiang , Ye Fengchun , Nguyen Kien , Karn Jonathan , McComsey Grace A. , McIntyre Thomas M. , Jin Ge 
Published: December 2022

AbstractElevated serum cytokine production in COVID-19 patients is associated with disease progression and severity. However, the stimuli that initiate cytokine production in patients remain to be fully revealed. Virus-infected cells release virus-associated exosomes, extracellular vesicles of endocytic origin, into the blood to deliver viral cargoes able to regulate immune responses. Here, we report that plasma exosomes of COVID-19 patients contain SARS-CoV-2 double stranded RNA (dsRNA) and stimulate robust production of interleukin-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α), and other inflammatory cytokines and chemokines by human peripheral mononuclear cells. Exosome depletion abolished these stimulated responses. COVID-19 plasma exosomes induced proinflammatory responses in CD4+ T cells, CD8+ T cells, and CD14+ monocytes but not significantly in regulatory T cells, Th17 T cells, or central memory T cells. COVID-19 plasma exosomes protect the SARS-CoV-2 dsRNA cargo from RNase and deliver the dsRNA into recipient cells. These exosomes significantly increase expression of endosomal toll-like receptor 3 (TLR3), TLR7, TLR8, and TLR9 in peripheral T cells and monocytes. A pharmacological inhibitor of TLR3 considerably reduced cytokine and chemokine production by CD4+ and CD8+ T cells but not by CD14+ monocytes, highlighting divergent signaling pathways of immune cells in response to COVID-19 plasma exosomes. Our results identify a novel model of intercellular crosstalk following SARS-CoV-2 infection that evoke immune responses positioned to contribute to elevated cytokine production associated with COVID-19 progression, severity, and long-haul symptoms.
Vaximap: route optimisation for housebound vaccination
Authors: Kirk Thomas F. , Barker Adam J. , Bodossian Armen , Staruch Robert 
Published: December 2022

AbstractDuring the United Kingdom’s Covid-19 vaccination campaign, general practitioners (GPs) have held responsibility for vaccinating housebound patients. This presented them with a large, complex and unfamiliar logistical challenge, namely determining the most time-efficient route to visit multiple patients at their home address. In response to a lack of existing solutions tailored specifically to vaccination, and in light of overwhelming demand, Vaximap (https://www.vaximap.org) was created in January 2021 to automate the process of route planning. It is free of charge for all users and has been used to-date to plan vaccinations for over 470,000 patients. This article analyses usage data to estimate the time savings (3 work years) and financial savings (£110,000) the service has yielded for GP surgeries, thus demonstrating that it helped to accelerate the UK’s Covid-19 vaccination campaign at critical moments.
Shapeshifting proteins: the role of structural disorder and conformational plasticity in physiology and disease
Authors: Mukhopadhyay Samrat 
Published: December 2022

Abstract
Intrinsically disordered proteins (IDPs) defy the conventional structure–function paradigm and do not autonomously fold up into unique 3D structures for carrying out functions. They exist as rapidly interconverting conformational ensembles and are thought to expand the functional repertoire of proteins. Such shapeshifting proteins are associated with a multitude of biological functions and a wide range of human diseases. The thematic issue on ‘Shapeshifting Proteins’ in Essays in Biochemistry includes some exciting and emerging aspects of this class of proteins. Articles in this issue provide current trends and contemporary views on various intriguing features of these proteins involving their unique structural and dynamical characteristics, misfolding and aggregation behavior, and their phase transitions into biomolecular condensates. I hope that this thematic issue will be of considerable interest to the practitioners in protein biochemistry and biophysics as well as to the researchers in other allied areas involving cell and molecular biology, neuroscience, virology, pathophysiology, and so forth.
Teaching Caregivers to Support Social Communication: Results From a Randomized Clinical Trial of Autistic Toddlers
Authors: Roberts Megan Y. , Stern Yael S. , Grauzer Jeffrey , Nietfeld Jennifer , Thompson Suzanne , Jones Maranda , Kaat Aaron J. , Kaiser Ann P. 
Published: December 2022

Background:
Studies of early caregiver-mediated interventions targeting social communication of young autistic children have yielded variable child outcomes. This study examined the effects of combining two caregiver-mediated interventions on caregiver strategy use and child social communication and language outcomes.

Method:

This was a multisite parallel randomized controlled trial. Participants included 120 caregivers and their autistic children between 24 and 36 months of age. Dyads were randomly assigned to receive a hybrid intervention that combined Enhanced Milieu Teaching (EMT) and Joint Attention, Symbolic Play, Engagement, and Regulation (JASPER) or to a behavior management control condition, each delivered over 6 months. Caregivers in the JASP-EMT group received twice-weekly, in-home, and hour-long sessions. Outcomes were measured at baseline, the end of intervention (T1), and 6 months later (T2) and included a naturalistic language sample procedure, standardized measures, and caregiver report measures. This trial was registered at
clinicaltrials.gov
(NCT02595697).

Results:
Child outcomes did not differ between conditions at T1 or T2 for child primary (social communication) or secondary (language, play, and autism symptoms) outcomes. Relative to control group caregivers, intervention group caregivers demonstrated significantly higher use of JASP-EMT strategies at T1 and T2, with the exception of two strategies (Responsiveness and Matched Responsiveness), which were used significantly more by control group caregivers. Neither autism severity nor baseline caregiver responsiveness moderated outcomes. Post hoc analyses revealed significant correlations between specific strategies and all child outcomes.

Conclusions:
Twice-weekly caregiver-mediated intervention that taught caregivers of autistic children to use social communication support strategies did not yield significant child outcomes. Future studies should examine possible sources for the lack of main effects including unexpected differences in linguistic features of caregiver input, changes in control group caregiver behavior, and insufficient intervention dosage.

Supplemental Material:

https://doi.org/10.23641/asha.21714278
Resultados do manejo de fraturas do rádio distal nos últimos 5 anos: metanálise de ensaios clínicos controlados e randomizados
Authors: Asmara Anak Agung Gede Yuda , Karna Made Bramantya , Meregawa Putu Feryawan , Deslivia Maria Florencia 
Published: December 2022

Resumo
Objetivo Nas últimas décadas, a placa volar bloqueada (PVB) tem sido o tratamento principal para fraturas do rádio distal (FRDs). Com o crescente conjunto de evidências, revisamos sistematicamente estudos sobre modificações recentes na PVB.
Métodos Uma pesquisa sistemática foi realizada utilizando o banco de dados PubMed/MEDLINE por estudos publicados em inglês nos últimos cinco anos. Os critérios de inclusão foram ensaios clínicos controlados e randomizados (ECCRs) sobre o tratamento cirúrgico de FRDs. Excluímos ensaios e estudos em andamento que não abordavam diretamente a FRD. Os desfechos primários avaliados foram desfechos clínicos subjetivos (como as pontuações no questionário de Deficiências do Braço, Ombro e Mão [Disabilities of the Arm, Shoulder and Hand, DASH, em inglês], na Avaliação do Punho Classificada pelo Paciente [Patient-rated Wrist Evaluation, PRWE, em inglês], no questionário Qualidade de Vida Europeia – 5 Dimensões [European Quality of Life–5 Dimensions, EQ-5D, em inglês], na Pesquisa de Saúde por Formulário Curto de 36 Itens [36-item Short Form Health Survey, SF-36, em inglês], e na Escala Visual Analógica [EVA]) e objetivos (taxa de complicações).
Resultados Identificamos 29 artigos publicados entre 2016 e 2020 com alta qualidade de evidência, exceto por um, de qualidade moderada. Ao todo, foram analisadas 3.079 FRDs neste estudo, Todos os estudos analisados, exceto por um, tinham maior proporção de participantes do gênero feminino, e somente em 1 estudo a idade média da amostra foi < 40 anos. Não houve diferença significativa entre a PVB e fixação externa (FE) em termos das pontuações no DASH (p = 0,18) e na PRWE (p = 0,77). Os resultados da PVB isolada, sem qualquer reparo do pronador quadrado (PQ), foram significativamente melhores.
Conclusão Em fraturas instáveis, a PVB e a FE produziram resultados comparáveis no longo prazo. Não há um benefício claro em se adicionar reparo do PQ à técnica cirúrgica atual da PVB.
Nível de Evidência Nível I
Frozen Elephant Trunk Sizing: A 30,000-Feet Perspective with Thoraflex Hybrid Stent Graft
Authors: Tan Sven Z. C. P. , Mohammed Idhrees , Bashir Mohamad 
Published: December 2022

AbstractThere is currently no standard, evidence-based approach for sizing the Thoraflex Hybrid prosthesis in frozen elephant trunk surgery. We present regional data on 906 Thoraflex grafts implanted in the United Kingdom between December 2012 and August 2021 to emphasize the heterogeneity in sizing practices and evaluate the impact this may have on clinical outcomes. Highlighting this heterogeneity will help develop an evidence-based approach to prosthesis sizing, thereby aiding decision-making for arch repair.
Alveolar Bone Loss in Diabetic Patients: A Case–Control Study
Authors: Tabassum Afsheen 
Published: December 2022

Abstract
Objectives Based on literature, very few case–control studies have been executed to confirm the relationship between diabetes mellitus and the severity of mean alveolar bone loss. Therefore, the aim was to assess the differences in mean alveolar bone loss among diabetic (type 2) and nondiabetic patients in the Saudi population.
Materials and Methods Nine-hundred eighty-two patient records were seen in this retrospective study. Patient demographic data and medical records were examined. The mean alveolar bone loss was measured in posterior teeth by calculating the distance from the base of cementoenamel junction to the alveolar crest using the bitewing radiographs. SPSS 20.0 was used for data analysis. An unpaired t-test was utilized to analyze the mean alveolar bone loss across multiple variables. p-Value less than or equal to 0.05 was contemplated as significant.
Results The overall mean alveolar bone loss for all 124 patients was 2.83 ± 1.13 mm. Diabetic patients had greater mean alveolar bone loss measured in millimeters than nondiabetic patients (3.07 ± 1.14mm vs. 2.59 ± 1.08mm, respectively), and the difference was significant (p = 0.018). In terms of the severity of mean alveolar bone loss, diabetic patients experience statistically higher mean alveolar bone loss as compared with nondiabetic patients.
Conclusion In our study population, the overall mean alveolar bone loss prevalence was greater in diabetes patients than in nondiabetic individuals. According to the severity of bone loss, the distribution of moderate and severe periodontitis was higher in diabetic patients. To enhance patients' quality of life, the awareness and education among patients regarding the association among diabetes mellitus and oral health, particularly periodontal disease, should be promoted.
Prevalence, Clinical Characteristics, and Treatment Response in Patients with Post Cricoid Inlet Patch—A Descriptive Retrospective Study
Authors: Ali Irshad H. , Bale Abhijith , Jalihal Umesh , C. Praveen Kumar A. , Bale Ajay , Sreenath Meghana 
Published: December 2022

Abstract
Objectives The aim of this study was to determine the incidence of post cricoid inlet patch (PC-IP) and to assess the clinical characteristics, pathological features, and treatment response.
Materials and Methods A retrospective cross-sectional study was conducted from April 2016 to April 2021 in the tertiary gastroenterology unit of urban India. All patients with symptoms of globus sensation, chronic cough, heartburn, hoarseness, throat pain, dysphagia, and acid regurgitation after a thorough clinical examination underwent esophagogastroduodenoscopy by experienced endoscopists including biopsy. Sociodemographic data, symptoms and its duration, previous hospital visits, and chronic proton pump inhibitor (PPI) use were noted during the study.
Results  Three-thousand two-hundred fifty upper gastrointestinal endoscopies were performed during the study period. The prevalence of PC-IP was 2.7%, comprising 36.3% males and rest females. Mean age was 36.2 ± 17years. The most common symptom among these patients was globus sensation (81.8%) followed by acid regurgitation (75%), dyspepsia (64.7%), dysphagia (48.8%), throat pain (29.54%), chronic cough (22.72%), hoarseness (22.72%), and others (6.81%). Mean diameter of PC-IP was 1.5 ± 0.5 cm, more than one patch was found in 23.76% of patients. Histopathological examination showed heterotrophic gastric mucosa in 77.27%, out of which 38.23% had oxyntic type, 44.11% had mucoid type, and the rest had mixed cell type. On median follow-up after 20 months, 68.18% of patient had persistent symptoms, 77.27% were PPI responsive, and 22.73% were PPI refractory. On follow-up, 38.63% of patients had a repeat endoscopic procedure and biopsy (20.45%); none showed any changes in size or dysplasia.
Conclusion Prevalence of PC-IP could be higher than the estimated. Careful examination of upper esophagus and use of narrow band imaging will increase the possibility of identifying IP. Those symptomatic patients need treatment with PPI, sometimes for long term. Ablative therapy with radiofrequency or argon plasma coagulation needs further clarification.
The Mediterranean diet and One Health: A study in synergies
Authors: Tangredi Basil P. 
Published: December 2022

Abstract
Food consumption patterns (diets) lie at the nexus of human wellness and environmental conservation. The Mediterranean diet, the traditional food and lifestyle patterns of the Mediterranean populations, is a plant-based whole foods cultural model of a healthy diet. The environmental benefits are rooted in the agro-silvo-pastoral farming systems promoting sustainable productivity, and conserving biodiversity and water resources, while also decreasing greenhouse gas emissions and future land clearing. Pasture-based husbandry results in improved animal health and welfare. Understanding the ancient hierarchy of synergies in this complex system can aid policy-makers, public health planners, agriculturists, and animal advocates meet present and future challenges.

One Health Impact Statement
Food choice can have a profound effect on human health and the environment. Less obvious is the impact on the health and welfare of food-producing animals. The Mediterranean dietary and lifestyle pattern is a plant-based whole foods diet, arising from sustainable agroecological practices, resulting in tangible benefits in all three domains. The synergistic aspects of each domain assemble into a complex system exemplifying the interdisciplinary concept of One Health that is crucial to meeting the challenges of today.
Innovative trends in reducing food waste and ensuring a more sustainable food system and environment
Authors: Areche Franklin Ore , Gondal Aqarab Husnain , Landeo Oliver Taype , Flores Denis Dante Corilla , Rodríguez Alfonso Ruiz , Pérez Peter Llimpe , Huaman Jovencio Ticsihua , Roman Almer Ventura , Correo Rafael Julian Malpartida Yapias 
Published: December 2022

Abstract
Food waste is one of the most important issues affecting modern food systems, with estimates indicating that almost one-third of food is either lost or wasted throughout the food supply chain, resulting in substantial environmental and socioeconomic consequences. We generate tonnes of edible and non-food wastes each year that is lost through inadequate storage or transportation, improper handling, adverse weather conditions, customer behaviour, a lack of cold storage or rejection in relation to aesthetic standards. Reduction in food loss and waste may result in more food for everyone, lower greenhouse gas emissions, less strain on water and land resources, higher productivity, economic development and more sustainable communities. This can be attained by adopting green, digital, innovative and enhanced technologies that are increasingly being used to prevent, reuse and recycle food waste. It has also been explored briefly how food waste might be recycled, waste minimised at source by technological options including machine learning etc. and utilised as a source of energy, biofuels, and nutrients in the agricultural industry. The present study discusses the technological advancement in nanotechnology, digital and green extraction technologies that are promising in the battle to decrease food waste and increase food security. It also explores the digital innovations in novel apps, platforms, social media for preventing and monitoring food waste and loss to raise public awareness. Finally, proposals for reducing waste through public awareness and necessary measures for governments are also presented.
Successful hospitality micro-entrepreneurs in Bandung, Indonesia
Authors: Abdullah Taufik , Carr Neil , Lee Craig 
Published: December 2022

Abstract
This case study investigates factors that contribute to the success of street food vendors based on interviews with vendors in Bandung, Indonesia. The study found that success for street food vendors is not exclusively related to gaining more financial and/or material possessions. Success can also be related to their business, individual and family well-being, and contribution to the community. The findings also show that achieving success for street food vendors can be attributed to several factors: networks, product and service quality, positive attitudes, and the utilization of technology.
Wronskian structures of planar symplectic ensembles
Authors: Byun Sung-Soo , Ebke Markus , Seo Seong-Mi 
Published: December 2022

Abstract
We consider the eigenvalues of non-Hermitian random matrices in the symmetry class of the symplectic Ginibre ensemble, which are known to form a Pfaffian point process in the plane. It was recently discovered that the limiting correlation kernel of the symplectic Ginibre ensemble in the vicinity of the real line can be expressed in a unified form of a Wronskian. We derive scaling limits for variations of the symplectic Ginibre ensemble and obtain such Wronskian structures for the associated universality classes. These include almost-Hermitian bulk/edge scaling limits of the elliptic symplectic Ginibre ensemble and edge scaling limits of the symplectic Ginibre ensemble with boundary confinement. Our proofs follow from the generalised Christoffel–Darboux formula for the former and from the Laplace method for the latter. Based on such a unified integrable structure of Wronskian form, we also provide an intimate relation between the function in the argument of the Wronskian in the symplectic symmetry class and the kernel in the complex symmetry class which form determinantal point processes in the plane.
Multi-step phase transitions and gravitational waves in the inert doublet model
Authors: Benincasa Nico , Delle Rose Luigi , Kannike Kristjan , Marzola Luca 
Published: December 2022

AbstractThe inert doublet model is a well-motivated extension of the Standard Model that contains a dark matter candidate and modifies the dynamics of the electroweak symmetry breaking. In order to detail its phenomenology, we perform a comprehensive study of cosmic phase transitions and gravitational wave signals implied by the framework, accounting for the latest results of collider experiments. We require the neutral inert scalar to constitute, at least, a subdominant part of the observed dark matter abundance. While most of the phase transitions proceed through a single step, we identify regions of the parameter space where the electroweak vacuum is reached after multiple phase transitions. The resulting gravitational wave spectrum is generally dominated by single-step transitions and, in part of the parameter space, falls within the reach of future gravitational wave detectors such as LISA, BBO or DECIGO. We find that direct detection experiments efficiently probe the part of parameter space associated with multi-step phase transitions, which remain unconstrained only in the Higgs resonance region testable with future monojet searches. The implications of the new determination of theWboson mass are also discussed.