bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interrogation of TERT promoter hotspot mutations in ameloblastoma and ameloblastic carcinoma
Authors: Magalhães Maria Carolina Silva Versieux , Felix Fernanda Aragão , Guimarães Letícia Martins , dos Santos Jean Nunes , de Marco Luiz Armando , Gomez Ricardo Santiago , Gomes Carolina Cavaliéri , de Sousa Sílvia Ferreira 
Published: September 2022

AbstractBackgroundTERT promoter mutations increase telomerase activity, conferring cell immortality. The coexistence of TERT promoter mutations with BRAFV600E is associated with aggressiveness. Ameloblastoma and ameloblastic carcinoma are infiltrative neoplasms that harbor BRAFV600E; however, it remains unknown if these odontogenic tumors also show TERT promoter mutations.MethodsGenomic DNA of paraffin‐embedded ameloblastomas (n = 6) and ameloblastic carcinomas (n = 3) were Sanger‐sequenced to assess the hotspot TERT promoter mutations C228T and C250T. BRAFV600E status was screened by TaqMan allele‐specific quantitative polymerase chain reaction.ResultsNone of the samples harbored TERT promoter mutations. The BRAFV600E mutation was positive in 3 of 6 of ameloblastomas and in 1 of 3 of ameloblastic carcinomas.ConclusionThe absence of TERT promoter mutation in the samples indicates that this molecular event is not relevant to the tumors' pathogenesis. Further studies are necessary to explore undefined genetic or epigenetic mechanisms related to TERT‐upregulation in ameloblastoma, and the telomerase activity in ameloblastic carcinoma.
A complete digital approach for facially generated full arch diagnostic wax up, guided surgery, and implant‐supported interim prosthesis by integrating 3D facial scanning, intraoral scan and CBCT
Authors: Amin Sara A. , Hann Sean , Elsheikh Ahmad Khater , Boltchi Farhad , Zandinejad Amirali 
Published: September 2022

AbstractContinuous innovation in digital dental technology offers new prospects for creating a complete virtual environment. The technique described adds a facial approach to the conventional digital workflow by incorporating 3D face scans to cone beam computed tomography and intraoral scans. Using this workflow, clinicians can obtain a complete virtual patient for facially generated diagnostic wax up and plan and implement a predictable implant placement and interim prosthesis. This technique provides a full digital workflow for restoratively‐driven computer‐aided implant planning, guided surgery, and 3D printing of an interim complete‐arch fixed implant‐supported prosthesis.
Expression of periodontitis susceptibility genes in human gingiva using single‐cell RNA sequencing
Authors: Caetano Ana J. , D'Agostino Eleanor M. , Sharpe Paul , Nibali Luigi 
Published: September 2022

AbstractObjectiveSingle‐cell transcriptomics was used to determine the possible cell‐type specificity of periodontitis susceptibility genes.BackgroundThe last decade has witnessed remarkable advances in the field of human genomics. Despite many advances, the genetic factors associated with or contributing to the periodontitis pathogenesis have only been identified to a limited extent and are often poorly validated. Confirming whether a given single nucleotide polymorphism has an association with periodontitis requires a robust mechanistic explanation on the functional consequences of a given genetic variant.MethodsWe globally assessed the expression of 26 disease‐associated genes identified by GWAS within the gingival mucosa. A total of 12 411 cells from 4 different donors were analysed. Differentially expressed genes were analysed using Seurat, a non‐parametric Wilcoxon rank sum test. The minimum threshold for significance was defined as p < .05.ResultsThis exploration at a cellular‐level suggests diverse populations contributing to disease pathogenesis, with macrophages expressing a higher number of the analysed disease‐associated genes. IL1B, PTGS2, FCGR2A, IL10 and IL1A specifically showed a more restricted expression in the myeloid lineages.ConclusionThis short report combines human genetics and single‐cell genomics to better understand periodontitis by mapping variants to predict their cells of action and putative functions. These findings seem to suggest that innate cell dysfunction is linked to disease susceptibility.
A prospective examination of swallow and cough dysfunction after lung transplantation
Authors: Dallal‐York Justine , Croft Kayla , Anderson Amber , DiBiase Lauren , Donohue Cara , Vasilopoulos Terrie , Shahmohammadi Abbas , Pelaez Andres , Pipkin Mauricio , Hegland Karen W. , Machuca Tiago N. , Plowman Emily K. 
Published: September 2022

AbstractObjectivesSwallow and cough dysfunction are possible surgical complications of lung transplantation (LT). We examined voluntary cough strength, sensorimotor reflexive cough integrity, and swallow‐related respiratory rate (RR) across swallowing safety and aspiration response groups in recovering LT recipients.MethodsForty‐five LT recipients underwent flexible endoscopic evaluation of swallowing indexed by the validated Penetration Aspiration Scale. RR before and after a 3‐ounce water drinking task was measured. Voluntary and reflexive cough screening were performed to index motor and sensory outcomes. T‐tests, one‐way ANOVAs, and chi‐square (odds ratios) were used.Results60% of patients exhibited laryngeal penetration (n = 27) and 40% demonstrated tracheal aspiration (n = 18); 72% (n = 13) demonstrated silent aspiration. Baseline RR was higher in aspirators versus non‐aspirators (26.5 vs. 22.6, p = 0.04) and in silent aspirators compared to non‐silent aspirators (27.9 vs. 20.7, p = 0.01). RR change post‐swallowing did not differ between aspiration response groups; however, it was significantly higher in aspirators compared to non‐aspirators (3 vs. −2, p = 0.02). Compared to non‐silent aspirators, silent aspirators demonstrated reduced voluntary cough peak expiratory flow (PEF; 166 vs. 324 L/min, p = 0.01). PEF, motor and urge to cough reflex cough ratings did not differ between aspirators and non‐aspirators. Silent aspirators demonstrated a 7.5 times higher odds of failing reflex cough screening compared to non‐silent aspirators (p = 0.07).ConclusionsDuring the acute recovery period, all LT participants demonstrated some degree of unsafe swallowing and reduced voluntary cough strength. Silent aspirators exhibited elevated RR, reduced voluntary cough physiologic capacity to defend the airway, and a clinically distinguishable blunted motor response to reflex cough screening.
Differential sensitivity of metabolic pathways in sugar beet roots to combined salt, heat, and light stress
Authors: García‐Caparrós Pedro , Vogelsang Lara , Persicke Marcus , Wirtz Markus , Kumar Vijay , Dietz Karl‐Josef 
Published: September 2022

AbstractPlants in nature commonly encounter combined stress scenarios. The response to combined stressors is often unpredictable from the response to single stresses. To address stress interference in roots, we applied salinity, heat, and high light to hydroponically grown sugar beet. Two main patterns of metabolomic acclimation were apparent. High salt of 300 mM NaCl considerably lowered metabolite amounts, for example, those of most amino acids, γ‐amino butyric acid (GABA), and glucose. Very few metabolites revealed the opposite trend with increased contents at high salts, mostly organic acids such as citric acid and isocitric acid, but also tryptophan, tyrosine, and the compatible solute proline. High temperature (31°C vs. 21°C) also frequently lowered root metabolite pools. The individual effects of salinity and heat were superimposed under combined stress. Under high light and high salt conditions, there was a significant decline in root chloride, mannitol, ribulose 5‐P, cysteine, and l‐aspartate contents. The results reveal the complex interaction pattern of environmental parameters and urge researchers to elaborate in much more detail and width on combinatorial stress effects to bridge work under controlled growth conditions to growth in nature, and also to better understand acclimation to the consequences of climate change.
Tweakments: Non‐surgical beauty technologies and future directions for the sociology of the body
Authors: Dowrick Anna , Holliday Ruth 
Published: September 2022

AbstractScience, aesthetics, the body, and the concerns they attach to, such as gender, ‘race’, class, age and consumer culture, are key objects of sociological investigation. These discourses have been reignited in recent years by changes in the availability, accessibility and affordability of medicalised cosmetic procedures. The most popular of these procedures are non‐surgical ‘tweakments’ to the shape and/or appearance of the face, usually through use of ‘injectables’ such as Botox and dermal fillers. This shift in focus from surgical procedures to minimally invasive injections has led more practitioners to join the market of potential providers and a wider variety of consumers to seek them out. This has been accompanied by panic about the risks to bodies and aesthetic standards if stewardship of beauty should fall into the wrong hands. We trace the history of medicalised cosmetic practices and academic discourses on the body, particularly how cosmetic practices are understood to produce the body as gendered and racialised. We then suggest future approaches for exploring the sociological significance of new cosmetic practices. We encourage researchers to explore how imaginaries of (un)desirable bodies shape debates about appropriate use of non‐surgical cosmetic procedures, alongside investigation of the situated intersections of identity that are inscribed on bodies.
Zygomatic arch reconstruction with apatient‐specificpolycaprolactone/beta‐tricalciumphosphate scaffold after parosteal osteosarcoma resection in a dog
Authors: Kang Kyu‐Won , Shim Jin‐Hyung , Kim Hyun‐Jung , Kang Byung‐Jae 
Published: September 2022

AbstractObjectiveTo describe the surgical application of a 3D–printing‐based, patient‐specific, biocompatible polycaprolactone/beta‐tricalcium phosphate (PCL/β‐TCP) scaffold to reconstruct the zygomatic arch after tumor resection in a dog.AnimalA 13 year old female spayed Maltese.Study designCase reportMethodsThe dog's presenting complaint was swelling ventral to her right eye. A round mass arising from the caudal aspect of the right zygomatic arch was identified by computed tomography (CT). The histopathologic diagnosis was a low‐grade spindle‐cell tumor. Surgical resection was planned to achieve 5 mm margins. A patient‐specific osteotomy guide and polycaprolactone/beta‐tricalcium phosphate (PCL/β‐TCP) scaffold were produced. Osteotomy, including 30% of total zygomatic arch length, was performed using an oscillating saw aligned with the guide. The scaffold was placed in the defect. Parosteal osteosarcoma was diagnosed based on histopathological examination. Excision was complete, with the closest margin measuring 0.3 mm.ResultsMild epiphora, due to surgical site swelling, subsided after 20 days. Tissue formation within and around the porous scaffold was noted on CT 10 months postoperatively, with no evidence of metastasis or local recurrence. Facial conformation appeared symmetrical, and no complications were noted 16 months postoperatively.ConclusionThe use of a 3D–printing‐based, patient‐specific, biocompatible PCL/β‐TCP scaffold successfully restored the structure and function of the zygomatic arch without complications, even following wide zygomectomy for complete tumor removal.
Indirect Tool Condition Monitoring Using Ensemble Machine Learning Techniques
Authors: Schueller Alexandra , Saldaña Christopher 
Published: September 2022

Abstract
Tool condition monitoring (TCM) has become a research area of interest due to its potential to significantly reduce manufacturing costs while increasing process visibility and efficiency. Machine learning (ML) is one analysis technique which has demonstrated advantages for TCM applications. However, the commonly studied individual ML models lack generalizability to new machining and environmental conditions, as well as robustness to the unbalanced datasets which are common in TCM. Ensemble ML models have demonstrated superior performance in other fields, but have only begun to be evaluated for TCM. As a result, it is not well understood how their TCM performance compares to that of individual models, or how homogeneous and heterogeneous ensemble models’ performances compare to one another. To fill in these research gaps, milling experiments were conducted using various cutting conditions, and the model groups were compared across several performance metrics. Statistical t-tests were also used to evaluate the significance of model performance differences. Through the analysis of four individual ML models and five ensemble models, all based on the processes’ sound, spindle power, and axial load signals, it was found that on average, the ensemble models performed better than the individual models, and that the homogeneous ensembles outperformed the heterogeneous ensembles.
Constructing Three-Dimensional Honeycomb Structures Based on Origami Geometry
Authors: Saito Kazuya , Fujimoto Akinobu , Okabe Yoji 
Published: September 2022

Abstract
Origami has shown the potential to design unique mechanical properties and complex three-dimensional shapes by folding through designed crease patterns on flat materials. The authors investigated a new honeycomb-based origami metamaterial called “kirigami honeycomb.” Resembling origami, kirigami honeycomb allows a single flat sheet of material with periodic slits to be folded into a honeycomb shape. Previous studies have reported successful use of this method to create various honeycomb shapes, changing only the folding line diagrams (FLDs). These previous studies have, however, considered only one-directional cross-sectional modifications; the core thickness and curvature changed only in the W-direction. This study proposes a new method that will support complex 3D honeycomb designs made from single flat sheets. A newly defined crease pattern conversion method provides arbitrary scaling of the honeycomb shape in the L-direction. The combined FLD and pattern conversion design methods encourage the cost-effective manufacture of 3D shaped honeycombs from single flat paper sheets. The proposed method is implemented to the design software, enabling to design arbitrary cross-sectional honeycomb cores with optional L-directional scaling.
Level of education is associated with coronary heart disease and chronic kidney disease in individuals with type 2 diabetes: a population-based study
Authors: Slåtsve Kristina B , Claudi Tor , Lappegård Knut Tore , Jenum Anne Karen , Larsen Marthe , Nøkleby Kjersti , Tibballs Katrina , Cooper John G , Sandberg Sverre , Buhl Esben Selmer , Løvaas Karianne Fjeld , Berg Tore Julsrud 
Published: September 2022

IntroductionTo study the relationship between education level and vascular complications in individuals with type 2 diabetes in Norway.Research design and methodsMultiregional population-based cross-sectional study of individuals with type 2 diabetes in primary care. Data were extracted from electronic medical records in the period 2012–2014. Information on education level was obtained from Statistics Norway. Using multivariable multilevel regression analyses on imputed data we analyzed the association between education level and vascular complications. We adjusted for age, sex, HbA1c, low-density lipoprotein cholesterol, systolic blood pressure, smoking and diabetes duration. Results are presented as ORs and 95% CIs.ResultsOf 8192 individuals with type 2 diabetes included, 34.0% had completed compulsory education, 49.0% upper secondary education and 16.9% higher education. The prevalence of vascular complications in the three education groups was: coronary heart disease 25.9%, 23.0% and 16.9%; stroke 9.6%, 7.4% and 6.6%; chronic kidney disease (estimated glomerular filtration rate <60 mL/min/1.73 m2) 23.9%, 16.8% and 12.6%; and retinopathy 13.9%, 11.5% and 11.7%, respectively. Higher education was associated with lower odds for coronary heart disease (OR 0.59; 95% CI 0.49 to 0.71) and chronic kidney disease (OR 0.75; 95% CI 0.60 to 0.93) compared with compulsory education when adjusting for age, sex, HbA1c, low-density lipoprotein cholesterol, systolic blood pressure, smoking and diabetes duration.ConclusionsIn a country with equal access to healthcare, high education level was associated with lower odds for coronary heart disease and chronic kidney disease in individuals with type 2 diabetes.
Efficacy of low-level laser therapy in patients with lower extremity tendinopathy or plantar fasciitis: systematic review and meta-analysis of randomised controlled trials
Authors: Naterstad Ingvill Fjell , Joensen Jon , Bjordal Jan Magnus , Couppé Christian , Lopes-Martins Rodrigo Alvaro Brandão , Stausholm Martin Bjørn 
Published: September 2022

ObjectivesWe investigated the effectiveness of low-level laser therapy (LLLT) in lower extremity tendinopathy and plantar fasciitis on patient-reported pain and disability.DesignSystematic review and meta-analysis.Data sourcesEligible articles in any language were identified through PubMed, Embase and Physiotherapy Evidence Database (PEDro) on the 20 August 2020, references, citations and experts.Eligibility criteria for selection of studiesOnly randomised controlled trials involving participants with lower extremity tendinopathy or plantar fasciitis treated with LLLT were included.Data extraction and synthesisRandom effects meta-analyses with dose subgroups based on the World Association for Laser Therapy treatment recommendations were conducted. Risk of bias was assessed with the PEDro scale.ResultsLLLT was compared with placebo (10 trials), other interventions (5 trials) and as an add-on intervention (3 trials). The study quality was moderate to high.Overall, pain was significantly reduced by LLLT at completed therapy (13.15 mm Visual Analogue Scale (VAS; 95% CI 7.82 to 18.48)) and 4–12 weeks later (12.56 mm VAS (95% CI 5.69 to 19.42)). Overall, disability was significantly reduced by LLLT at completed therapy (Standardised Mean Difference (SMD)=0.39 (95% CI 0.09 to 0.7) and 4–9 weeks later (SMD=0.32 (95% CI 0.05 to 0.59)). Compared with placebo control, the recommended doses significantly reduced pain at completed therapy (14.98 mm VAS (95% CI 3.74 to 26.22)) and 4–8 weeks later (14.00 mm VAS (95% CI 2.81 to 25.19)). The recommended doses significantly reduced pain as an add-on to exercise therapy versus exercise therapy alone at completed therapy (18.15 mm VAS (95% CI 10.55 to 25.76)) and 4–9 weeks later (15.90 mm VAS (95% CI 2.3 to 29.51)). No adverse events were reported.ConclusionLLLT significantly reduces pain and disability in lower extremity tendinopathy and plantar fasciitis in the short and medium term. Long-term data were not available. Some uncertainty about the effect size remains due to wide CIs and lack of large trials.PROSPERO registration numberCRD42017077511.
Profile and treatment outcomes among young patients with tuberculosis aged 15–24 years in Faridabad district of Haryana, India
Authors: Kamble Bhushan Dattatray , Malhotra Sumit 
Published: September 2022

ObjectiveThis study examined profile and treatment outcomes of young patients with tuberculosis (TB) registered at a district TB centre under the National TB Elimination Programme in Faridabad district of Haryana state in India.MethodsIn this secondary data analysis, we studied the records of young TB patients aged 15–24 years registered under a TB programme during October 2013–December 2017 in Faridabad district of Haryana state.ResultsWe analysed records of 5257 young patients with TB. Majority (58.7%) were patients with pulmonary TB and most of them (83.6%) were registered as new patients. Majority of the young patients with TB (93.2%) had a successful treatment outcome, and patients having sputum result 2+ or less and patients who did not have a previous history of TB were found to be significantly associated with a successful treatment outcome on multivariable analysis.ConclusionThere was a high treatment success rate noted in young patients with TB. More focus is needed to patients having a history of TB and sputum result >2+. Targeted interventions can be designed for these groups in future programmatic strategies to reduce disease burden in this section of young population.
What is the feasibility and patient acceptability of a digital system for arm and hand rehabilitation after stroke? A mixed-methods, single-arm feasibility study of the ‘OnTrack’ intervention for hospital and home use
Authors: Fusari Gianpaolo , Gibbs Ella , Hoskin Lily , Lawrence-Jones Anna , Dickens Daniel , Fernandez Crespo Roberto , Leis Melanie , Crow Jennifer , Taylor Elizabeth , Jones Fiona , Darzi Ara 
Published: September 2022

ObjectivesArm weakness is common after stroke; repetitive activity is critical for recovery but people struggle with knowing what to do, volume, and monitoring progress. We studied the feasibility and acceptability of OnTrack, a digital intervention supporting arm and hand rehabilitation in acute and home settings.DesignA mixed-method, single-arm study evaluating the feasibility of OnTrack for hospital and home use. An independent process evaluation assessed the intervention’s fidelity, dose and reach. Amendments to the protocol were necessary after COVID-19.SettingAcute stroke services and home settings in North West London.Participants12 adults with a stroke diagnosis <6 months previously (first or recurrent) requiring arm rehabilitation in hospital and/or home.Intervention12 weeks using the OnTrack system comprising arm tracking and coaching support for self-management.Primary and secondary outcome measuresRecruitment, retention and completion rates; compliance and adherence to the intervention; reasons for study decline/withdrawal.Intervention fidelity and acceptability, evaluated through an independent process evaluation.Patient measures including activity baseline, healthcare activation, arm function and impairment collected at baseline, week 7 and week 14 of participation to assess suitability for a randomised controlled trial (RCT).Results181 individuals screened, 37 met eligibility criteria, 24 recruited (65%); of these, 15 (63%) were recruited before COVID-19, and 9 (37%) during. 12 completed the intervention (50%). Despite COVID-19 disruptions, recruitment, retention and completion were in line with prestudy expectations and acceptable for a definitive trial. Participants felt the study requirements were acceptable and the intervention usable. Fidelity of delivery was acceptable according to predetermined fidelity markers. Outcome measures collected helped determine sample size estimates and primary outcomes for an RCT.ConclusionsThe intervention was found to be usable and acceptable by participants; study feasibility objectives were met and demonstrated that a definitive RCT would be viable and acceptable.Trial registration numberNCT03944486.