bluuebunny/crossref_metadata_2025_split huggingface dataset:
METTL3‐mediated maturation of miR‐589‐5p promotes the malignant development of liver cancer
Authors: Liu Jie , Jiang Kai 
Published: March 2022

AbstractMiR‐589‐5p could promote liver cancer, but the specific mechanisms are largely unknown. This study examined the role and mechanisms of miR‐589‐5p in liver cancer. The expressions of miR‐589‐5p, METTL3 and m6A in liver cancers were determined by RT‐qPCR. The relationship between miR‐589‐5p and METTL3‐mediated m6A methylation was examined by m6A RNA immunoprecipitation. After transfection, the viability, migration, invasion and expressions of METTL3 and miR‐589‐5p in liver cancer cells were detected by CCK‐8, wound‐healing, transwell and RT‐qPCR. After the xenograft tumour was established in mice, the tumour volume was determined and the expressions of METTL3, miR‐589‐5p, MMP‐2, TIMP‐2, E‐cadherin, N‐cadherin and Vimentin in tumour tissue were detected by RT‐qPCR and Western blotting. In vitro study showed that miR‐589‐5p and METTL3 were highly expressed in liver cancer. METTL3 was positively correlated with miR‐589‐5p. METTL3 up‐regulated the expression of miR‐589‐5p and promoted the maturation of miR‐589‐5p. Overexpressed miR‐589‐5p and METTL3 promoted the viability, migration and invasion of liver cancer cells, while the effects of silencing miR‐589‐5p and METTL3 on the cells were the opposite. The effects of METTL3 overexpression and silencing were reversed by miR‐589‐5p inhibitor and mimic, respectively. In vivo study showed that METLL3 silencing inhibited the growth of xenograft tumour and the expressions of METTL3, MMP‐2, N‐cadherin and Vimentin, promoted the expressions of TIMP‐2 and E‐cadherin, while miR‐589‐5p mimic caused the opposite results and further reversed the effects of METLL3 silencing. In summary, this study found that METTL3‐mediated maturation of miR‐589‐5p promoted the malignant development of liver cancer.
OsDMI3‐mediated OsUXS3 phosphorylation improves oxidative stress tolerance by modulating OsCATB protein abundance in rice
Authors: Ni Lan , Wang Qingwen , Chen Chao , Wang Shuang , Shen Tao , Jiang Jingjing , Cui Zhenzhen , Li Kaiyue , Yang Qiqing , Jiang Mingyi 
Published: March 2022

AbstractCalcium (Ca2+)/calmodulin (CaM)‐dependent protein kinase (CCaMK) is an important positive regulator of antioxidant defenses and tolerance against oxidative stress. However, the underlying molecular mechanisms are largely unknown. Here, we report that the rice (Oryza sativa) CCaMK (OsDMI3) physically interacts with and phosphorylates OsUXS3, a cytosol‐localized UDP‐xylose synthase. Genetic and biochemical evidence demonstrated that OsUXS3 acts downstream of OsDMI3 to enhance the oxidative stress tolerance conferred by higher catalase (CAT) activity. Indeed, OsUXS3 interacted with CAT isozyme B (OsCATB), and this interaction was required to increase OsCATB protein abundance under oxidative stress conditions. Furthermore, we showed that OsDMI3 phosphorylates OsUXS3 on residue Ser‐245, thereby further promoting the interaction between OsUXS3 and OsCATB. Our results indicate that OsDMI3 promotes the association of OsUXS3 with OsCATB to enhance CAT activity under oxidative stress. These findings reveal OsUXS3 as a direct target of OsDMI3 and demonstrate its involvement in antioxidant defense.
Professional identity and supporting willingness of nurses during the COVID‐19 epidemic in China
Authors: Li Li , Gao Xiaoyue , Wang Yanbo , Zeng Changjuan , Hou Lili , Xi Qinghong 
Published: March 2022

AbstractAimTo investigate the professional identities and the willingness of nurses to respond to the call for support during the COVID‐19 epidemic.BackgroundThe COVID‐19 epidemic has resulted in nearly 300 million cases worldwide, causing more than five million deaths. However, the professional identities and the willingness of nurses to provide support during the COVID‐19 epidemic in China remain unclear.MethodsA total of 1,505 eligible nurses from 120 hospitals during the COVID‐19 outbreak in China were included. Questionnaires were used to evaluate the willingness of these nurses to participate in epidemic control efforts. The Nurses' Professional Identity Scale was used to measure their sense of professional identity.ResultsAbout 90% of the nurses were willing to lend support in Hubei Province during the epidemic. The most common reason (93.84%) was found to be their beliefs as medical personnel in helping others in need. Nearly 10% of the nurses were unwilling to go to Hubei, primarily due to family reasons. The average total score of the Professional Identity Scale for all nurses reached a moderate to high level as indication of professional identity (116–125). Nurses who were willing to go to Hubei had a significantly higher total score than those who were not.ConclusionsThe professional identity of nurses in China improved during the epidemic, and those with higher professional identities were more likely to respond to calls for support during the epidemic.
The ins and outs of the caudal nucleus of the solitary tract: An overview of cellular populations and anatomical connections
Authors: Holt Marie K. 
Published: March 2022

AbstractThe body and brain are in constant two‐way communication. Driving this communication is a region in the lower brainstem: the dorsal vagal complex. Within the dorsal vagal complex, the caudal nucleus of the solitary tract (cNTS) is a major first stop for incoming information from the body to the brain carried by the vagus nerve. The anatomy of this region makes it ideally positioned to respond to signals of change in both emotional and bodily states. In turn, the cNTS controls the activity of regions throughout the brain that are involved in the control of both behaviour and physiology. This review is intended to help anyone with an interest in the cNTS. First, I provide an overview of the architecture of the cNTS and outline the wide range of neurotransmitters expressed in subsets of neurons in the cNTS. Next, in detail, I discuss the known inputs and outputs of the cNTS and briefly highlight what is known regarding the neurochemical makeup and function of those connections. Then, I discuss one group of cNTS neurons: glucagon‐like peptide‐1 (GLP‐1)‐expressing neurons. GLP‐1 neurons serve as a good example of a group of cNTS neurons, which receive input from varied sources and have the ability to modulate both behaviour and physiology. Finally, I consider what we might learn about other cNTS neurons from our study of GLP‐1 neurons and why it is important to remember that the manipulation of molecularly defined subsets of cNTS neurons is likely to affect physiology and behaviours beyond those monitored in individual experiments.
Development of severe pemphigus vulgaris following ChAdOx1 nCoV‐19 vaccination and review of literature
Authors: Singh Ajeet , Bharadwaj Sujana J. , Chirayath Anju G. , Ganguly Satyaki 
Published: March 2022

AbstractVaccines are indeed a boon for tackling the present COVID‐19 pandemic. In India, ChAdOx1 nCoV‐19 (Covishield) is the most commonly used vaccine in the government vaccination program for adults more than 18 years of age. It is a recombinant vaccine developed by Oxford‐Astra Zeneca and manufactured in India by Serum Institute of India (SSI). Here, we report a case of severe pemphigus vulgaris following the second dose of ChAdOx1 nCoV‐19 vaccination in an adult male. The patient developed septicemia during the course of hospital stay, and he was managed with systemic steroids, parenteral antibiotics, and intravenous immunoglobulins (IVIg) along with proper wound care. Patient started improving within 1 month of therapy. This case is being reported in view of the rarity of pemphigus vulgaris following ChAdOx1 nCoV‐19 vaccine.
Hepatitis C virus infection could be a risk factor for adult‐onset vitiligo in Egyptian patients: A cross‐sectional study
Authors: Fawzy Manal M. , Hammad Noha M. , Sharaf Ahmed L. , Khattab Fathia 
Published: March 2022

AbstractBackgroundVitiligo is a common skin disorder resulting from the destruction of melanocytes. Hepatitis C virus (HCV) infection has been linked to a variety of extrahepatic manifestations, including skin diseases.AimTo measure the prevalence of HCV‐seroreactivity among vitiligo patients.MethodsThis cross‐sectional study included 108 vitiligo patients. Serum anti‐HCV antibodies were detected by chemiluminescence immunoassay.ResultsEighteen patients (16.7%) out of 108 were HCV‐reactive; all of them had adult‐onset vitiligo. They represented approximately 34.6% of the total patients with adult‐onset vitiligo (52 patients). On the contrary, all patients with childhood‐onset vitiligo were HCV‐non‐reactive. Moreover, adult‐onset vitiligo was significantly associated with HCV‐seroreactivity (p < .001). Also, there was a significant difference between HCV‐reactive and HCV‐non‐reactive vitiligo patients regarding the age of patients and their ages at the onset of vitiligo (p < .001).ConclusionsHCV may be the triggering factor for adult‐onset vitiligo, particularly in regions with a high prevalence of HCV. Therefore, patients with adult‐onset vitiligo, rather than childhood‐onset disease, should be screened for associated HCV infection in HCV‐endemic regions.
Adverse effects of non‐intubated airway suctioning: a clinical data‐based study
Authors: Chang Wen‐Pei , Chen Hsiao‐Mei , Wu Jia‐Rong , Tsai Hsiu‐Ting , Ho Chiung‐Fang , Lin Yu‐Huei 
Published: March 2022

AbstractAims and objectivesThe purpose of this study was to investigate the adverse effects of non‐intubated suctioning.BackgroundAirway hygiene aims to maintain a patent airway to ensure adequate ventilation. Nasopharyngeal, oropharyngeal or nasotracheal suctioning may be used in patients who require airway suctioning but do not have an artificial airway. However, no studies till date provide insight into the adverse effects of non‐intubated airway suctioning.DesignA clinical data‐based retrospective design.MethodUsing institutional and clinical databases of three university hospitals in 2008–2016, we conducted a study with a propensity score matching method of 3,326 hospitalised patients who had undergone suction therapy with or without a tracheotomy. Conditional logistic regression analyses were performed to investigate the link between suctioning and the probabilities of adverse effects. STROBE checklist was used to report the current study.ResultsPatients who required nasopharyngeal, oropharyngeal or nasotracheal suctioning had a higher risk of gastrointestinal ulcers than tracheotomised patients (adjusted OR 1.99; 95% CI, 1.24–3.20). Patients who received non‐intubated suction had a higher risk of developing pneumonia (adjusted OR 1.59; 95% CI, 1.26–2.00), and the risk of aspiration pneumonia was three times higher than tracheotomised patients (adjusted OR 3.04; 95% CI, 1.40–6.60).ConclusionsNon‐intubated patients who require suctioning for airway clearing are more susceptible to gastrointestinal ulcers, pneumonia and aspiration pneumonia. The findings would facilitate in alerting healthcare professionals to this group of patients. However, more clinical research is needed to elucidate the mechanisms of adverse effects in non‐intubated patients who require suctioning.Relevance to Clinical PracticeThe adverse effects of suctioning can easily be overlooked in debilitated patients with no intubation. Professionals must be aware of the discomfort and risks that patients may experience.
The oral mycobiome: Oral epithelial dysplasia and oral squamous cell carcinoma
Authors: Theofilou Vasileios Ionas , Alfaifi Areej , Montelongo‐Jauregui Daniel , Pettas Efstathios , Georgaki Maria , Nikitakis Nikolaos G. , Jabra‐Rizk Mary‐Ann , Sultan Ahmed S. 
Published: March 2022

AbstractFungi, a diverse group of eukaryotic organisms, play distinct roles in health and disease. Recent advances in the field of mycobiology have enabled the characterization of the “human mycobiome.” The human mycobiome has extensively been studied in various disease models. However, to date, the role of the oral mycobiome in oral carcinogenesis has yet to be elucidated. Candida albicans, the most common oral colonizer, has been speculated to display tumorigenic effects; however, the literature lacks consistent documentation from mechanistic studies on whether oral mycobiota act as drivers, facilitators, or passive colonizers of oral premalignancy and cancer. This review article provides an overview of existing hypothesis‐driven mechanistic models that outline the complex interplay between the oral mycobiome and oral epithelial dysplasia as well as their potential clinical implications.
Oral and maxillofacial manifestations of IgG4‐related disease: A clinicopathological study
Authors: Pereira Glauce Guimarães , Pontes Flávia Sirotheau Corrêa , Soares Ciro Dantas , de Carvalho Maria Goretti Freire , da Silva Tarcília Aparecida , Calderaro Débora Cerqueira , Ferreira Gilda Aparecida , Tanure Leandro Augusto , de Souza Lucas Lacerda , Rodrigues‐Fernandes Carla Isabelly , de Almeida Oslei Paes , Fonseca Felipe Paiva , Pontes Hélder Antônio Rebelo 
Published: March 2022

AbstractBackgroundIgG4‐related disease is a fibroinflammatory and immune‐mediated condition, which has extremely variable clinical manifestations. In this study, we aim to investigate the clinicopathological features of IgG4‐related disease involving the oral and maxillofacial region.MethodsCases of IgG4‐related disease manifesting in the oral and maxillofacial region were retrieved from three Brazilian institutions. Clinical and serological data were obtained from the patients' medical charts, while microscopic and immunohistochemical findings were revised by oral pathologists. Diagnosis followed the American College of Rheumatology/European League against Rheumatism criteria.ResultsSeven patients diagnosed with IgG4‐related disease were included in this study. Women were affected in all analysed cases, with a mean age of 55.4 years. Two patients presented with the clinical involvement of more than one oral and maxillofacial anatomic site. Therefore, our sample comprised nine oral and maxillofacial anatomic sites affected by IgG4‐related disease. The submandibular gland was affected in four cases, the tongue and the parotid gland in two cases each, and the palate in one case. In a few cases, exploratory lower lip biopsy was used as a diagnostic approach. A moderate‐to‐severe lymphoid infiltrate containing plasma cells and lymphocytes, with an increased IgG4/IgG ratio, was common. Treatment varied and steroids were the most frequently used (57.4%). Six patients remained alive, while one died from unknown causes.ConclusionAlthough major salivary glands are commonly affected by IgG4‐related disease, the oral cavity can also be involved, and lower lip biopsy may be an auxiliary diagnostic tool.
Effects of reminiscence therapy in people with dementia: A systematic review and meta‐analysis
Authors: Saragih Ita Daryanti , Tonapa Santo Imanuel , Yao Ching‐Teng , Saragih Ice Septriani , Lee Bih‐O 
Published: March 2022

Accessible SummaryWhat is known about on the subject?

Alternative option was developed to improve care due to the increasing costs of care cost and the number of people diagnosed with dementia.

Reminiscence therapy is a commonly implemented alternative option used in long‐term care facilities.

Reminiscence therapy is designed for cognitive decline that is also known as life review.

What this paper adds to existing knowledge?

Reminiscence therapy, known as psychosocial interventions in dementia care, can be used to assist people with dementia recollect prior events, activities, and experiences in order to improve their cognitive, mood, and overall well‐being.

Reminiscence therapy increased cognitive function and quality of life and reduced depressive and neuropsychiatric symptoms among people with dementia.

Reminiscence therapy may be considered a useful non‐pharmacological intervention for people with dementia living in nursing homes or other long‐term care facilities.

The development of a global standard protocol for the application of reminiscence therapy may be necessary for future randomized controlled trials (RCTs).

AbstractIntroductionReminiscence therapy is an alternative to pharmaceutical intervention provided during long‐term care, especially for individuals with dementia. However, the effects of reminiscence therapy in dementia care remain inconclusive.AimThe goal of this study is to examine the effects of reminiscence therapy implementation in people with dementia.DesignSystematic literature review and meta‐analysis were conducted in accordance with the PRISMA guidelines.MethodsThis study searched systematically using 6 databases. The eligibility criteria included patients with dementia, applied reminiscence therapy, randomized controlled trials or quasi‐experimental studies, and published in the English language. The PEDro scale was used to assess the methodological quality of the included studies. The meta‐analysis was performed using a random‐effects model to calculate the pooled effects of reminiscence therapy. Stata 16.0 was used for statistical analysis.ResultA total of 29 studies met the eligibility criteria, including 3102 participants. Overall, reminiscence therapy increased cognitive functions and quality of life and decreased depression and neuropsychiatric symptoms.Implication for PracticeReminiscence therapy may be considered a useful non‐pharmacological intervention for people with dementia living in nursing homes or other long‐term care facilities. A standard protocol for reminiscence therapy may be necessary for future studies.
Amyloid neuropathy and autonomic dysfunction
Authors: Ikeda Shu‐ichi 
Published: March 2022

AbstractThe clinical phenotype of amyloid neuropathy is not uniform. Especially in patients with hereditary amyloid neuropathy, the age at onset and severity of autonomic neuropathy are strongly influenced by the patient’s family history and the mutant gene of transthyretin. In the past 30 years, liver transplantation has been actively performed in the hereditary amyloid neuropathy patients with an early onset, resulting in much longer survival with this disease. Furthermore, new information on the pathogeneses of transthyretin‐derived amyloidosis has been accumulating via the analysis of tissue samples biopsied or autopsied from post‐transplanted hereditary amyloid neuropathy patients. The recent introduction of non‐invasive drug therapies has given rise to the possibility of treating amyloid neuropathy. The concept of amyloid neuropathy, including autonomic dysfunction, has changed. This review discusses the clinical picture, pathology, diagnosis, therapy, and post‐treatment changes of amyloid deposition in patients with amyloid neuropathy and autonomic neuropathy.
Importation of chloroplast proteins under heat stress is facilitated by their SUMO conjugations
Authors: Zheng Xiao‐Ting , Wang Caijuan , Lin Wenxiong , Lin Chufang , Han Danlu , Xie Qi , Lai Jianbin , Yang Chengwei 
Published: March 2022

Summary

Chloroplasts are hypersensitive to heat stress (HS). SUMOylation, a critical post‐translational modification, is conservatively involved in HS responses. However, the functional connection between SUMOylation and chloroplasts under HS remains to be studied.

The bioinformatics, biochemistry, and cell biology analyses were used to detect the SUMOylation statuses of Arabidopsis nuclear‐encoded chloroplast proteins and the effect of SUMOylation on subcellular localization of these proteins under HS. PSBR, a subunit of photosystem II, was used as an example for a detailed investigation of functional mechanisms.

After a global SUMOylation site prediction of nuclear‐encoded chloroplast proteins, biochemical data showed that most of the selected candidates are modified by SUMO3 in the cytoplasm. The chloroplast localization of these SUMOylation targets under long‐term HS is partially maintained by the SUMO ligase AtSIZ1. The HS‐induced SUMOylation on PSBR contributes to the maintenance of its chloroplast localization, which is dependent on its chloroplast importation efficiency correlated to phosphorylation. The complementation analysis provided evidence that SUMOylation is essential for the physiological function of PSBR under HS.

Our study illustrated a general regulatory mechanism of SUMOylation in maintaining the chloroplast protein importation during HS and provided hints for further investigation on protein modifications associated with plant organelles under stress conditions.
Mandibular condyle changes in rats with unilateral masticatory function
Authors: De Carli Enrico , Lagou Aikaterini , Kiliaridis Stavros , Denes Balazs J. 
Published: March 2022

AbstractObjectiveCertain malocclusions or unilateral tooth loss can lead to asymmetric functional load of the two mandibular sides during mastication and induce skeletal asymmetries to the condylar process of growing individuals. However, in adults, asymmetric function may have a different impact. The aim of the present study was to investigate three‐dimensionally the effects of unilateral masticatory function on the condylar process morphology in growing and adult rats and the adaptive processes to differential condylar loading.Materials and MethodsFifty‐six growing and adult Wistar rats aged 4 and 26 weeks respectively were obtained. The maxillary right molars of the experimental animals were extracted and all animals were followed for 12 weeks. Three‐dimensional images were obtained by an in‐vivo microcomputed tomography (micro‐CT) examination. The following measurements were studied: condylar process height, condylar base width, and condylar cross‐sectional surface.ResultsWhile no differences were found with regards to condylar process height and base width, the cross‐section of the condyle on the extraction side did not increase during growth in the young rats. No such differences were found in adults. Young rats had statistically significantly shorter condylar height, base width and cross‐sectional surface than the adult rats and showed significant growth of these structures during the experimental period.ConclusionCondylar height and base width growth are not hindered by reduced occlusal function, contrary to the average cross‐sectional surface, which implies that the condyle form of growing individuals becomes thinner while maintaining its length, in the absence of occlusal stimuli. The condyle of adult rats with extractions is less affected by occlusion changes.
Effect of telmisartan on an experimental model of periodontitis in mice
Authors: Jeon So Jung , Lee Yohan , Keum Bo Ram , Choi Eun‐Young , Choi In Soon , Kim Sung‐Jo 
Published: March 2022

AbstractObjectiveThis study was performed to explore the impact of telmisartan on experimental periodontitis in mice, in terms of alveolar bone destruction, by using micro‐computed tomography analysis.Materials and methodsMale BALB/c mice were divided into four groups of 7 to 9 mice each: control (C) group; experimental periodontitis (E) group; experimental periodontitis‐plus‐telmisartan 5 mg/kg (ET5) group; and experimental periodontitis‐plus‐telmisartan 10 mg/kg (ET10) group. The mice in Group C were not subjected to experimental periodontitis. The other mice from Groups E, ET5 and ET10 were exposed to periodontitis. Periodontitis was induced by inoculation with Porphyromonas gingivalis.ResultsTelmisartan significantly suppressed both the reduction in alveolar bone height and increase of root exposure caused by P. gingivalis infection. When mice were treated with telmisartan, the decrease in the bone volume fraction induced by the infection was notably recovered. In addition, telmisartan reversed P. gingivalis‐induced alterations in the microstructural parameters of trabecular bone, except trabecular thickness.No significant difference was evident between Groups ET5 and ET10 in both the extent of alveolar bone loss and microstructural parameters assessed, except bone volume fraction and trabecular number.ConclusionTelmisartan may have potential benefits as a host modulation agent for the therapy of periodontitis.