bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ca2+-responsive phospholipid-binding BONZAI genes confer a novel role for cotton resistance to Verticillium wilt
Authors: Hao Xiaoyan , Gao Shengqi , Chen Guo , Li Jianping , Zhao Zhun , Hu Wenran , Huang Quansheng 
Published: September 2022

Abstract

Verticillium wilt which produced by the soil-borne fungus Verticillium dahliae is an important biotic threat that limits cotton (Gossypium hirsutum) growth and agricultural productivity. It is very essential to explore new genes for the generation of V. dahliae resistance or tolerance cotton varieties. Ca2+ signaling as a secondary messenger is involved in pathogen stress response. Despite Ca2+-responsive phospholipid-binding BONZAI (BON) genes have intensively been investigated in Arabidopsis, their function has not still been characterized in cotton. Here, we show that three copies of GhBON1, two copies of GhBON2 and GhBON3 were found from the genome sequences of upland cotton. The expression of GhBON1 is inducible to both V. dahliae and salicylic acid (SA), jasmonic acid (JA) and H2O2 stresses. Knocking down of GhBON1, GhBON2 and GhBON3 using virus induced gene silencing (VIGS) each increases up-regulation of defense responses in cotton. These GhBON1, GhBON2 and GhBON3-silenced plants enhance resistance to V. dahliae accompanied by higher burst of hydrogen peroxide and increased cell death and have more effect on the up-regulation of defense response genes. Further analysis revealed that GhBON1 could interacts with BAK1-interacting receptor-like kinase 1 (GhBIR1) and pathogen-associated molecular pattern (PAMP) receptor regulator BAK1 (GhBAK1) at plasma membrane. Our study further reveals that plant Ca2+-responsive phospholipid-binding BONZAI genes negatively regulate Verticillium wilt with the conserved function in response to disease resistance or plant immunity.
Loss of Lkb1 disturbs adipose tissue dendritic cells and protects mice from obesity under high-fat feeding condition
Authors: Sun Yunyan , Wang Bing , Hu Qianwen , Lai Xun , Wang Tier , Zhao Chunxiao , Wang Jiali , Zhang Xi , Niu Qing , He Baolin , Shi Mingxia , Feng Xiaoming , Luo Yuechen 
Published: September 2022

Abstract
Adipose tissue CD11c + myeloid cell is an independent risk factor associated with obesity and metabolic disorders. However, the underlying molecular basis remains elusive. Here, we demonstrated that liver kinase B1 (Lkb1), a key bioenergetic sensor, is involved in CD11c + cell-mediated immune responses in diet-induced obesity. Loss of Lkb1 in CD11c + cells results in obesity resistance but lower glucose tolerance, which accompanies tissue-specific immune abnormalities. The accumulation and CD80’s expression of Lkb1 deficient adipose-tissue specific dendritic cells but not macrophages is restrained. Additionally, the balance of IL-17A and IFN-γ remarkably tips towards the latter in fat T cells and CD11c - macrophages. Mechanistically, IFN-γ promotes apoptosis of preadipocytes and inhibits their adipogenesis, which might account in part for the fat-resistant phenotype. In summary, these findings reveal that Lkb1 is essential for fat CD11c + dendritic cells responding to HFD exposure and provides new insights into the IL-17A/IFN-γ balance in HFD-induced obesity.
Flexible kesterite thin-film solar cells under stress
Authors: Jo William , Park Ha Kyung , Cho Yunae , Kim Juran , Kim Sammi , Kim Sungjun , Kim Jeha , Yang Kee-Jeong , Kim Dae-Hwan , Kang Jin-Kyu 
Published: September 2022

Abstract
Understanding the stress-induced physical phenomena is essential for improving the long-term application of flexible solar cells to non-flat surfaces on buildings and in mobile utilities. Here, we investigated the electronic band structure and carrier transport mechanism of Cu2ZnSn(S,Se)4 (CZTSSe) photovoltaic devices under mechanical stress. We fabricated highly efficient flexible CZTSSe devices on a Mo metal foil substrate by controlling the incorporation of Na. When mechanical stress was applied to the CZTSSe material, electronic structure of CZTSSe was deformed as the band gap, valence band edge, and work function increased or decreased. Additionally, electrical properties of bent CZTSSe surface were probed by Kelvin prove force microscopy and the CZTSSe with Na doping showed less degraded carrier transport in the bending state (either concave or convex) compared to the CZTSSe without Na. Furthermore, the local open-circuit voltage (VOC) on the CZTSSe surface decreased under mechanical stress due to limited carrier excitation. The reduction of local VOC with mechanical bending occurred larger with convex bending than in concave bending, which is consistent with the degradation of device parameters with bending. This study paves the way for understanding the stress-induced optoelectronic changes in flexible photovoltaic devices beyond the kesterite-based devices.
Donor cord blood aging accelerates in recipients after transplantation
Authors: Onizuka Makoto , Imanishi Tadashi , Harada Kaito , Aoyama Yasuyuki , Amaki Jun , Toyosaki Masako , Machida Shinichiro , Kikkawa Eri , Yamada Sanetoshi , Nakabayashi Kazuhiko , Hata Kenichiro , Higashimoto Ken , Soejima Hidenobu , Ando Kiyoshi 
Published: September 2022

Abstract
Cord blood stem cell transplantation is an important alternative for patients needing hematopoietic stem cell transplantation. However, it is unclear how cord blood cells, which are 0-year-old, age in the recipient’s body after allogeneic transplantation. We performed DNA methylation (DNAm) age analysis to measure the age of cells using post-transplant peripheral blood in 50 cases of cord blood transplantation. The median chronological age (the time elapsed from the date of the cord blood transplant to the day the sample was taken for DNAm analysis) of donor cells was 4.0 years (0.2 – 15.0 years), while the median DNAm age was 10.0 years (1.3 – 30.3 years), and the ratio of DNAm age to chronological age (AgeAccel) was 2.7 (1.2 – 8.2). When comparing the mean values of AgeAccel in cord blood transplant cases and controls, the values were significantly higher in cord blood transplant cases. The characteristics of patients and transplant procedures were not associated with AgeAccel in this analysis, nor were they associated with the development of graft-versus-host disease. However, this analysis revealed that transplanting 0-year-old cord blood into a recipient resulted in cells aging more than twice as quickly as the elapsed time. The results shed light on the importance of the mismatch between cord blood stem cells and donor environmental factors in stem cell aging.
Investigation into a practical approach and application of cotton fiber elongation
Authors: Delhom Christopher D , Wanjura John D. , Pelletier Mathew G. , Holt Gregory A , Hequet Eric F. 
Published: September 2022

Abstract
The strength of cotton fiber has been extensively studied and significantly improved through selective breeding, but fiber elongation has largely been ignored, even though elongation contributes to determining the energy needed to break fibers. Recent developments to calibrate the High Volume Instrument (HVI) for elongation has renewed interest in elongation. However, it is not understood how best to utilize yet another fiber property which has the potential to add to the complexity of fiber selection. To explore a practical approach to applying elongation, cotton samples were tested using single fiber methods, the Stelometer, and the HVI. Comparison of strength, elongation, and combined properties such as modulus were explored. HVI testing was shown to be sensitive enough to characterize elongation differences but unlike single fiber testing it was unable to capture within-sample variation. Fiber bundle testing, like Stelometer and HVI was shown to reduce bias due to fiber selection. The use of secant modulus, an intrinsic material property, allowed for one value to represent both strength and elongation. Secant modulus was shown to contain more useful information than either elongation or work-to-break. Work-to-break was shown to be more influenced by a specific value of breaking force or elongation rather than the intrinsic behavior of the sample being tested.Exploring the influence of genetics and environment on elongation, and its interaction with other fiber properties, requires additional work. Secant modulus, by combining strength and elongation into one value, shows the potential to incorporate elongation values into fiber characterization without increasing the complexity of current fiber selection processes.
Experimental investigation on the expansive characteristics of Gansu clay-sulfate rocks in China subjected to confined compression and cyclic drying-wetting
Authors: Cheng Shufan , Zeng Yawu , Ye Yang , Gao Rui 
Published: September 2022

Abstract

Gansu clay-sulfate rock is a special red-bed rock that formed in an evaporation environment with strong oxidation. Due to the high content of hydrophilic minerals (including clay mineral, sulfate, and carbonate), tunnel in clay-sulfate rock stratum has a potential risk of large expansive deformation after the erosion of groundwater. In this study, a loadable cyclic drying-wetting instrument was designed to simulate the occurrence of the surrounding rock mass of a tunnel experiencing a periodic change in groundwater level. Firstly, a series of drying-wetting cycle tests with different normal pressure were carried on to obtain the expansion regulation of clay-sulfate rock. Then, computed tomography (CT) and scanning electron microscope (SEM) tests were carried on to explain the corresponding mechanism in mesoscopic. The test results show that large expansive deformation was generated in the first water immersion and only part of the deformation can be recovered during the later drying process. During the 2–5 cycles, expansion-shrink deformation is much less than in the first cycle, and the volume of the sample tends to be constant. Meanwhile, the degeneration of the clay-sulfate rock was observed in the first cycle by the CT and SEM test. Then the mesostructure of the sample is stable during the 2–5 cycle. It indicates the clay rock, in large part, degenerated to clay soil during the first drying-wetting cycle. Structural degradation and hydration of anhydrite is the main source of irreversible expansive deformation. Moreover, normal stress and confined restraint can reduce or even eliminate the expansive deformation, however, cannot avoid the degeneration of mesostructure. The shear strength of Gansu clay-sulfate rock also decreased after the first drying-wetting cycle, which manifested as the attenuation in both cohesion and internal friction angle.
KDM6 demethylases integrate DNA repair gene regulation and loss of KDM6A sensitizes human acute myeloid leukemia to PARP and BCL2 inhibition
Authors: Boila Liberalis Debraj , Ghosh Subhadeep , Bandyopadhyay Subham , Lin Liqing , Murison Alexander , Zeng Andy , Shaikh Wasim , Bhowmik Satyaki , Muddineni Siva Sai Naga Anurag , Biswas Mayukh , Sinha Sayantani , Chatterjee Shankha Subhra , Mbong Nathan , Gan Olga , Bose Anwesha , Chakraborty Sayan , Arruda Andrea , Kennedy James , Mitchell Amanda , Lechman Eric , Banerjee Debasis , Milyavsky Michael , Minden Mark , Dick John , Sengupta Amitava 
Published: September 2022

Abstract
Acute myeloid leukemia (AML) is a heterogeneous, aggressive malignancy with dismal prognosis and with limited availability of targeted therapies. Epigenetic deregulation contributes to AML pathogenesis. KDM6 proteins are histone-3-lysine-27-demethylases that play context-dependent roles in AML. We inform that KDM6-demethylase function critically regulates DNA-damage-repair-(DDR) gene expression in AML. Mechanistically, KDM6 expression is regulated by genotoxic stress, with deficiency of KDM6A-(UTX) and KDM6B-(JMJD3) impairing DDR transcriptional activation and compromising repair potential. Acquired KDM6A loss-of-function mutations are implicated in chemoresistance, although a significant percentage of relapsed-AML has upregulated KDM6A. Olaparib treatment reduced engraftment of KDM6A-mutant-AML-patient-derived-xenografts, highlighting synthetic lethality using Poly-(ADP-ribose)-polymerase-(PARP)-inhibition. Crucially, a higher KDM6A expression is correlated with venetoclax tolerance. Loss of KDM6A increased mitochondrial activity, BCL2 expression, and sensitized AML cells to venetoclax. Additionally, BCL2A1 associates with venetoclax resistance, and KDM6A loss was accompanied with a downregulated BCL2A1. Corroborating these results, dual targeting of PARP and BCL2 was superior to PARP or BCL2 inhibitor monotherapy in inducing AML apoptosis, and primary AML cells carrying KDM6A-domain-mutations were even more sensitive to the combination. Together, our study illustrates a mechanistic rationale in support for a novel combination therapy for AML based on subtype-heterogeneity, and establishes KDM6A as a molecular regulator for determining therapeutic efficacy.
Delay in healthcare seeking and associated factors among caregivers of children aged 2-59 months with pneumonia in public health facilities ofNekemte city, Western Ethiopia, 2022
Authors: Temsesgen Dereje , Wordofa Berhanu , Tesfaye Tewodros , Etafa Werku 
Published: September 2022

Abstract
Background:Pneumonia is an infectious disease that is the predominant cause of death among under-five children (U5C) in low- and middle-income countries. It is the second leading cause of death in Ethiopia. Delay in healthcare seeking is one of the contributing factors to pneumonia-associated mortality. There is a limitation to the study aimed at identifying health-seeking behavior and risk factors in the Western part of Ethiopia. The study aims to identify the level of delay in healthcare seeking and associated factors among caregivers of U5C in public health facilities in Nekemte city, Western Ethiopia.
Methods: A health facility-based cross-sectional study was conducted from 1st March to 5th April 2022 using a structured interviewer-administered questionnaire from 410 caregivers of children aged 2–59 months. We used a Systematic sampling technique for collecting the data. Data were entered into Epi Data version 4.6.06 and exported to SPSS version 25 for analysis. Binary logistic regression was used to identify the associated factors of delay in healthcare seeking at a p-value < 0.05 using a 95 % confidence interval (CI).
Results: A proportion of delay in healthcare seeking was 62.2%.Rural residence (AOR= 2.77, 95% CI: 2.48-5.17), child aged ≥12months (AOR: 5.4; 95 %CI: 4.17-7.20), family’s
monthly income of less than 1000 ETB (AOR: 6.11, 95% CI: 2.16-17.26, p=0.001), not using health insurance (AOR: 8.93; 95 %CI: 5.43-14.68), use of self-medication (AOR: 10.97; 95% CI: 1.85-65.3), poor knowledge (AOR: 4.63; 95% CI: 1.35-15.9), perceiving illness as mild (AOR:14.97; 95% CI: 9.76-22.9) and no previous admission history (AOR: 2.85; 95 % CI 1.77-4.56).
Conclusion: The study emphasizes that caregivers' delays in healthcare seeking are high. Creating caregivers' awareness or providing adequate health education to develop early healthcare seeking behavior and encouraging caregivers to use health insurance is essential.
SWAT model application for sediment yield modeling and parameters analysis in Wadi K’sob (Northeast of Algeria)
Authors: Maref Noureddine , Baahmed Djelloul , Bemmoussat Kader , Mahfoud Zakaria 
Published: September 2022

Abstract

A comprehension of the erosion processes and sediment transport in the watershed is essential for the sustainable management of the water resources and soil fertility.
In this study, the Water Assessment Tool (SWAT) model was applied to demonstrate its ability to modeling the suspended sediment transport in the Wadi K’sob basin (Northeast of Algeria). The data used to set up the SWAT model are the Digital Elevation Model (DEM), land use, soil types and weather data.
The calibration was carried out using daily sediment yield data corresponding to two (2) periods( 10/10/2006 to 06/17/2007 and 10/29/2012 to 10/22/2013) and validation was performed using two (2) other periods (06/18/2007 to 11/19/2007 and 10/23/2013 to 09/13/2014) of daily sediment yield. R-SWAT code was used for calibration process and sensitivity analysis. The performance of the SWAT model was evaluated by the Nash–Sutcliffe criterion (NSE) and coefficient of determination (R2).
The results obtained after calibration oscillating between good and satisfactory where (NSE = 0.67 and R2 = 0.73) in the first calibration period and (NSE = 0.65 b and R2 = 0.67) in the second period. For validation, the performance of the SWAT model was very good (NSE = 0.78 and R2 = 0.79) in the first period while in the second period the prediction of the model was satisfactory (NSE = 0.52 and R2 = 0.54).
In addition, the validation process revealed that some parameters are stable and related on watershed characteristics while other unstable parameters depend on soil properties especially soil permeability and soil erodibility.
Does intercropping of legumes with oilseeds modify mycorrhizal colonisation?
Authors: Dowling Alyce , Roberts Penny , Zhou Yi , Denton Matt 
Published: September 2022

Abstract

Background and Aims Legume-oilseed intercrops are increasingly grown in mechanised agricultural systems for their improved nutrient use efficiency. However, the mechanisms that underpin this advantage are not well known. This study aimed to investigate the effect of intercropping and species mixture on the arbuscular mycorrhizal fungi (AMF) colonisation of oilseed and legume crops, and subsequent effects on crop phosphorus nutrition.
Methods We sampled legume-oilseed intercrops in field experiments and measured the level of AMF root colonisation and shoot phosphorus. Additionally, we grew legume-oilseed intercrops in the glasshouse using AMF-inoculated ( Rhizophagus irregularis ) and mock-inoculated treatments. Measurements included mycorrhizal colonisation, root and shoot biomass, and shoot phosphorus.
Results Mycorrhizal colonisation and the subsequent effect on phosphorus nutrition was host plant dependent. Lentil was the most mycorrhizal plant, followed by linseed, chickpea, and then canola. Only in lentil in the glasshouse was there a correlation between mycorrhizal colonisation and shoot phosphorus (R = 0.79, p<0.001). Intercropping reduced mycorrhizal colonisation of lentil in the glasshouse but not in the field; intercropping did not affect AMF colonisation in any other species. The interaction between intercropping and AMF had a limited effect on crop growth and shoot phosphorus, while intercropping alone increased canola shoot phosphorus.
Conclusion The role of AMF in the growth and phosphorus nutrition of legume-oilseed intercropping systems appears host specific, and lacks a “one size fits all” solution. Research should be directed towards host plant-AMF specificity, and field studies using diverse soil P profiles.
Giving and receiving thanks: A mixed methods pilot study of a gratitude intervention for palliative patients and their carers
Authors: Bernard Mathieu , Poncin Emmanuelle , Bovet Emilie , Tamches Emmanuel , Cantin Boris , Pralong Josiane , Borasio Gian Domenico 
Published: September 2022

Abstract
Background
Psychological research examining the nature and workings of gratitude has burgeoned over the past two decades. However, few studies have considered gratitude in the palliative care context. Based on an exploratory study which found that gratitude was correlated with better quality of life and less psychological distress in palliative patients, we designed and piloted a gratitude intervention where palliative patients and a carer of their choice wrote and shared a gratitude letter with each other. The aims of this study are to establish the feasibility and acceptability of our gratitude intervention and provide a preliminary assessment of its effects.
Methods
This pilot intervention study adopted a mixed-methods, pre-post evaluation design. To assess the intervention’s effects, we employed quantitative questionnaires on quality of life, quality of relationship, psychological distress, and subjective burden, as well as semi-structured interviews. To assess feasibility, we considered patients and carers’ eligibility, participation and attrition rates, reasons for refusal to participate, appropriateness of intervention timeframe, modalities of participation, and barriers and facilitators. Acceptability was assessed through post-intervention satisfaction questionnaires.
Results
Thirty-nine participants completed the intervention. We did not find any statistically significant pre/post intervention changes for patients, but found significant decrease in psychological distress for carers in terms of depression (median = 3 at T0, 1.5 at T1, p = .034) and total score (median = 13 at T0, 7.5 at T1, p = .041). Qualitative analysis indicates that overall, the intervention had: (1) multiple positive outcomes for 11 participants, in the form of positive emotional, cognitive, and relational effects; (2) simple positive outcomes for 14 participants, who experienced emotional or cognitive effects; (3) no effect on two patients; and (4) negative emotional effects on two patients. Feasibility and acceptability indicators suggest that the intervention was well received by participants, and that it should adopt flexible modalities (e.g. writing or dictating a gratitude message) to ensure that it is feasible and adapted to individual needs and preferences.
Conclusions
Larger scale deployment and evaluation of the gratitude intervention, including a control group, is warranted in order to have a more reliable evaluation of its effectiveness in the palliative care setting.
Physical, mechanical and biological properties of composite bone cements containing silver-doped bioactive and antibacterial glass particles with different particles size
Authors: Miola Marta , Lucchetta Giovanni , Verné Enrica 
Published: September 2022

Abstract

In the present work, an antibacterial composite bone cement is designed introducing a bioactive and antibacterial glass into a commercial formulation. The effect of glass particles insertion on the curing parameters is evaluated; moreover, the influence of the glass particle size (< 20 µm or between 20 and 45 µm) on the glass dispersion, compressive and bending strength, bioactivity and antibacterial effect is estimated. The results evidence a delay in the polymerization kinetics of composite cements, which nevertheless comply with the requirements of the ISO standard. The morphological characterization evidence a good dispersion of the glass in the polymeric matrix and its exposition on the cement surface. The different glass grain size does not affect the composites bioactivity and compression strength; while a slight reduction on bending strength is observed for samples containing glass powders between 20 and 45 µm. The size of the glass particles also appears to have an effect on antibacterial properties, since the composites containing larger glass particles do not produce inhibition halo towards S. aureus strain. The obtained results demonstrate that, by carefully tailoring the glass amount and size, a multifunctional device for the artificial joints fixing, temporary prostheses or spinal surgery can be obtained.