bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the Application of Noiseless Steganography and Elliptic Curves Cryptography Digital Signature Algorithm Methods in Securing Text Messages
Authors: Juhari Juhari , Andrean Mohamad Febry 
Published: November 2022

Elliptic curve cryptography includes symmetric key cryptography systems that base their security on mathematical problems of elliptic curves. There are several ways that can be used to define the elliptic curve equation that depends on the infinite field used, one of which is the infinite field prima ( where ). Elliptic curve cryptography can be used for multiple protocol purposes, digital signatures, and encryption schemes.The purpose of this study is to determine the process of hiding encrypted messages using the Noiseless Steganography method as well as the generation of private keys and public keys and the process of verifying the validity of the Elliptic Curves Cryptography Digital Signature Algorithm (ECDSA). The result of this thesis is that a line graph is obtained that store or hides a message using the steganography method and a message authenticity from the process of key generation and verification of validity using the ECDSA method. By selecting three samples consisting of one test sample and two differentiating samples, a line graph, an MD5 hash value, and a value at the point are obtained different. Successively obtained values to message “Matematika 2018”, “MATEMATIKA 2018”, and “2018 matematika” are and . The discussion in this thesis only covers the elliptic curves on prime finite field. So, for the next thesis, the next researcher can do a discussion about the elliptic curve on the finite field or the application of elliptic curve cryptography and other steganographic methods
Apport de la Fracturation et des Méthodes d’analyses Statistiques à l’etude Hydrogéochimique des Eaux Souterraines du Massif Calcareux de la Région de Tlata Taghramt, Chaîne Calcaire du Haouz (Rif Septentrional, Maroc)
Authors: Barhi Hassan , Stitou El Messari Jamal Eddine , Oulad Mansour Mohamed Reda 
Published: November 2022

L’objectif de cette étude vise à établir les caractéristiques hydrogéologiques et hydrochimiques des eaux souterraines des formations carbonatées de la région de Tlata Taghramt. Au total, 16 échantillons d'eau ont été prélevés pour l’analyse chimique. Le traitement des données hydrochimiques a été réalisé à l'aide de diagrammes de Piper, les indices de saturation en minéraux et à base des méthodes d’analyses statistiques. Les paramètres physiques tels que la conductivité électrique, le potentiel hydrogène et les températures ont été mesurés sur le terrain à savoir que : la conductivité électrique varie entre 403μS/cm et 925μS/cm, le potentiel hydrogène varie entre 7.55 et 8.04 et les températures varient entre 15°C et 18°C. L'analyse hydrochimique montre une abondance en éléments majeurs (Anions et Cations) respectivement par ordre décroissant : (HCO3-> Cl- > SO42- > NO3-) et (Ca2+ > Mg2+ >Na+ > K+). Les bicarbonates représentent 70% du total des anions, tandis que le calcium représente 12% du total des cations. Cet enrichissement en ces deux éléments traduit la nature carbonatée des formations géologiques traversées par les eaux souterraines ainsi l'interaction de l'eau avec la matrice du système aquifère. L'étude de la fracturation indique une dominance de fractures orientées N-S favorisant l’apparition des sources via les joints et les sutures affectant les roches réservoirs. De point de vue chimique, les eaux des sources ont une nature généralement bicarbonatée calcique et magnésienne.

This study aims to establish the hydrogeological and hydrochemical characteristics of groundwater of carbonate rocks in the Tlata Taghramt region. A total of 16 water samples were collected for chemical analysis. Hydrochemical data processing was carried out using Piper diagrams, mineral saturation indices and statistical analysis methods. Physical parameters such as Electrical Conductivity, PH and Temperatures were measured in the field. The EC varies between 403μS/cm and 925μS/cm, the PH varies between 7.55 and 8.04 and the temperatures vary between 15°C and 18°C. Hydrochemical analysis shows an abundance of major elements (Anions and Cations) respectively in descending order: (HCO3-> Cl- > SO42- > NO3-) and (Ca2+ > Mg2+ >Na+ > K+). Bicarbonates account for 70% of total anions, while calcium accounts for 12% of total cations. This enrichment in these two elements reflects the carbonate nature of the geological formations crossed by the groundwater and the interaction of water with the aquifer system matrix. The study of fracturing indicates a dominance of N-S oriented fractures favoring the appearance of sources via joints and sutures affecting reservoir rocks. From a chemical point of view, spring waters have a calcium and magnesium bicarbonate-type facies.
KNOWLEDGE DEFICIT ABOUT DIETARY NEED IN FAMILIES WITH GOUT ARTHRITIS: A CASE STUDY
Authors: Wijayanti Endah Sri , Iswatun Iswatun , Sairozi Ali , Lestari Feby Diah , Lutfiandini Cherlys Tin 
Published: November 2022

Introduction: At this time, people with gout arthritis are not only found in the elderly because lifestyles that pay less attention to their health also have a risk of increased uric acid levels in the blood. Gout arthritis is a joint disease caused by increased uric acid levels in the blood, causing several problems. One of the causes of gout is the patient's lack of knowledge about the rules of the gout arthritis diet. The purpose of this study was to carry out family nursing care with a deficiency of understanding of case gout arthritis in Balungtawun Village, Sukodadi District of Lamongan Regency. Methods: The research method used is case study research. The research subjects used five respondents from five family members who had gouty arthritis with knowledge deficit problems in Balungtawun Village, Sukodadi District of Lamongan Regency, using a family nursing process approach. The data was collected through interviews, observation, physical examination, and documentation. The data obtained were analyzed by comparing the theory with cases. Results: The results of the case studies were analyzed, and it was found that there was a match in the process of assessment, diagnosis, intervention, and evaluation. Meanwhile, there are gaps in implementation that are not by the planned intervention. Conclusions: It is expected that families and patients can apply the proper diet for gout arthritis to reduce recurrence and uric acid levels in the patient's blood.
THE RELATIONSHIP OF HAND WASHING BEHAVIOR TOWARDS DIARRHEA CASES IN SCHOOL-AGE CHILDREN
Authors: Ade Nuraeni  , Rosiah Rosiah , Megga Putri Kemala Supendi  , Adhan Efendi  
Published: November 2022

Introduction: Hand washing is one of the current clean and healthy living behaviours that is a world concern. The problem of bad-hand washing behaviour not only occurs in developing countries but also in developed countries where most people still forget the importance of washing hands. The prevalence of diarrhoea among children aged 5-14 years reaches 182.338 (6.2%). The objective of this study was to determine the relationship between hand-washing behaviour and cases of diarrhoea in school-age children at Angkasa 2 Elementary School. Methods: This research method is quantitative, with a descriptive correlation research design, and uses a cross-sectional approach. The sample in this study was 63 respondents taken by purposive sampling. Data collection used questionnaires and observation sheets, analyzing data using a Chi-square test. Results: 73.01% of respondents had good hand-washing behaviour, while 26.98% had less hand-washing behaviour. Then, the respondents with good diarrhoea cases were 69.84%, and those with fewer diarrhoea cases were 30.15%. The statistical test showed a relationship between the variables of hand-washing behaviour with cases of diarrhoea (p =<0.001). Conclusions: There is a relationship between hand-washing behaviour and cases of diarrhoea in school-age children at Angkasa 2 elementary school. Hand washing is the most important basic technique in preventing and controlling the transmission of diarrheal diseases. It is necessary to have good facilities and knowledge about hand-washing, and the delivery of messages must be done recur so that understanding can align with these behavioural practices.
Assessment of the Variation Range of Agglutinability in <i>Vibrio cholerae</i> Strains Isolated in the Course of Monitoring Studies
Authors: Levchenko D. A. , Kruglikov V. D. , Arkhangel’skaya I. V. , Yakusheva O. A. , Alekseeva L. P. , Vodop’yanov S. O. , Ezhova M. I. , Noskov A. K. 
Published: November 2022

The aim of the study was to retrospectively analyze the range of variability of antigenic properties and genotypic characteristics of Vibrio cholerae R-variant strains atypical in terms of agglutinability.Materials and methods. 169 strains of V. cholerae R-variant with atypical agglutinability have been studied using the “AmpliSens® Vibrio cholerae-FL” test-system. The determination of O1 antigen was carried out using the “Ig-V. cholerae О1/О139 – ELISA/dot-ELISA” reagent kit.Results and discussion. A retrospective analysis of the complex of phenoand genotypic characteristics of strains isolated from surface water bodies in the territories of three former Soviet republics and 13 constituent entities of the Russian Federation in the course of 30-year monitoring and identified upon isolation as nontoxigenic V. cholerae R-variant strains has been performed. Upon re-identification, it was found that the strains belong to both epidemically dangerous (3.0 %) and non-dangerous strains (97.0 %). The range of variability was expressed in their distribution into three groups and consisted in retaining of agglutinability only with cholera RO serum in the first group (34.5 % of strains); the loss of this trait, but the acquisition of the ability to agglutinate in different combinations with O1, Ogawa or Inaba sera – in the second (16.7 %); and also in the loss of agglutinability with all diagnostic cholera sera – in the third (48.8 %). The presence of the wbeT gene in the compared V. cholerae classical R-variant strain does not exclude the presence of the genomic region for O1 antigen biosynthesis in other R-strains, possibly in a modified form, which can be clarified in further molecular-genetic studies. Alternatively, such strains are likely to be attributed to V. cholerae nonO1/nonO139. Strains of V. cholerae R-variant with different amounts of surface antigen (optical density range – from 0.088±0.002 to 1.226±0.003) have been identified. The data obtained can be used for monitoring of cholera in laboratories of regional and federal levels.
Anthrax in the Republic of Tatarstan (1920–2020)
Authors: Savitskaya T. A. , Trifonov V. A. , Milova I. V. , Isaeva G. Sh. , Reshetnikova I. D. , Serova I. V. , Lopushov D. V. , Ziatdinov V. B. 
Published: November 2022

The aim of the work was to characterize the epidemiological and epizootic situation on anthrax among population and animals in the Republic of Tatarstan over a period of 1920–2020.Materials and methods. The analysis of the epidemiological and epizootic situation is based on the archival data, epidemiological maps of anthrax patients, results of epizootiological-epidemiological survey of anthrax foci conducted by the Rospotrebnadzor Administration in the Republic of Tatarstan and Center of Hygiene and Epidemiology in the Republic of Tatarstan, materials of the Main Directorate of Veterinary Medicine of the Republic of Tatarstan. Microbiological studies of samples from patients and environmental objects were performed in accordance with the requirements of MR 4.2.2413-08 “Laboratory diagnostics and detection of anthrax pathogen”, real-time PCR was set using the AmpliSense Bacillus anthracis-FRT test-system (Central Research Institute of Epidemiology, Moscow). Statistical data processing was carried out using the quantile ranking method.Results and discussion. There are more than 1000 anthrax soil foci in the Republic of Tatarstan, which territorially belongs to the Volga Federal District. Analysis of the epizootic and epidemiological situation in the Republic of Tatarstan over the period of 1920–2020 has revealed that it has undergone significant changes, from mass diseases in animals and humans in early 20th century to sporadic cases of infection among population and animals at the beginning of the 21st century, primarily due to preventive veterinarysanitary measures, including veterinary and sanitary examination of animal products, mass specific immunization of animals against anthrax, arrangement of anthrax cattle burial grounds. In view of the improvement of epizootiological situation and implementation of preventive measures, there was a decrease in the incidence of anthrax among the population. The regions of the Republic have been ranked by the number of animal anthrax cases.
Comparative Analysis of Potential Determinants of Resistance to Aminoglycosides in <i>Burkholderia pseudomallei</i> Strains with Different Level of Sensitivity to Gentamicin
Authors: Chirskov P. R. , Bui T. L.A. , Ustinov D. V. , Viktorov A. D. , Zakharova I. B. 
Published: November 2022

The aim of the study was to identify and compare potential determinants of aminoglycoside resistance in gentamicin susceptible Burkholderia pseudomallei strains.Materials and methods. A bioinformatics analysis of whole genome shotgun sequences of three B. pseudomallei strains having different levels of sensitivity to gentamicin was carried out.Results and discussion. B. pseudomallei is intrinsically resistant to aminoglycosides. Such strains, as a rule, are not taken into account in the classical scheme of isolation and identification. At the same time, there were no significant differences in the clinical manifestations of melioidosis during infection with gentamicin-resistant and sensitive strains. In B. pseudomallei strains of different sequence types (ST70, ST948, and ST1566), point missense mutations were found in the genes of three efflux pumps of the RND family: AmrAB-OprA, BpeAB-OprB, BpeEF-OprC, and one with unknown functions, as well as in the gene aminoglycoside-6’-N-acetyltransferase AAC(6’)-III. All three strains had amino acid substitutions in the AmrA periplasmic linker: ARG160SER, Arg116Gln and Gly237Arg, Thr317Lys, respectively. In moderately sensitive strains (ST948 and ST1566), an identical Val222Met substitution was found in the repressor of the AmrAB-OprA operon, AmrR. It is likely that the intermediate level of sensitivity to gentamicin in the studied strains is mediated by the constitutive expression of the AmrAB-OprA operon, which partially compensates for the structural defects. It is also possible that a dinucleotide deletion in the AAC (6’)-III aminoglycoside-6’-N-acetyltransferase gene, as well as detected mutations in the homologues of the periplasmic linker (BPSL2234) of an uncharacterized efflux operon of the RND family, are involved in the loss of resistance to gentamicin.
Pathogenicity of the SARS-CoV-2 Virus Variants of Concern for the Syrian Golden Hamster
Authors: Shipovalov A. V. , Kudrov G. A. , Tomilov A. A. , Bodnev S. A. , Boldyrev N. D. , Ovchinnikova A. S. , Zaikovskaya A. V. , Taranov O. S. , Ivleva E. K. , P’yankov O. V. , Maksyutov R. A. 
Published: November 2022

The aim of the work was to study the pathogenicity of newly emerging variants of SARS-CoV-2 on the model of the Syrian golden hamster.Materials and methods. We used the strains of SARS-CoV-2 virus related to the VOC circulating in the territory of the Russian Federation. The experiments were carried out on outbreed Syrian hamsters obtained from the nursery of the SSC VB “Vector”. The infectious titer of coronavirus in tissue samples collected from infected laboratory animals was determined on a Vero E6 cell culture. The Ct in RT-PCR was considered an additional parameter for monitoring the viral load in the samples. The severity of lung tissue damage in Syrian hamsters with COVID-19 was assessed by histological preparations.Results and discussion. 50 % infecting doses in case of the intranasal infection have been determined, histological analysis of lung tissues performed. The pathogenicity of various variants of the SARS-CoV-2 virus for the Syrian hamster has been evaluated, differences in infecting doses and pathological changes in the lungs have been revealed. SARS-CoV-2 viruses belonging to Beta genetic variant have the highest virulence, while Alpha variant has the lowest one when comparing the studied strains by the ID50 value. The Delta and Omicron variants have a matched ability to cause specific damage to the tissues of the respiratory tract, while being inferior only to the Beta variant. It has been demonstrated that Syrian hamsters are an adequate model for assessing the pathogenicity of the SARS-CoV-2 virus variants of concern. Variants of SARS-CoV-2 virus during intranasal infection has shown different degree of pathogenicity in the Syrian hamster model.
Classification of Municipalities in the Republic of Buryatia by the Level of Tick-Borne Viral Encephalitis Incidence
Authors: Ayugin N. I. , Andaev E. I. , Nikitin A. Ya. , Khankhareev S. S. , Istomina T. F. 
Published: November 2022

Tick-borne viral encephalitis (TBVE) is one of the most significant natural-focal infections in the Russian Federation.The aim of the study was to analyze the current epidemiological situation on TBVE in the Buryat Republic in 2010–2020 with a subsequent differentiation of municipalities by epidemiological risk groups in order to elaborate proposals for optimization of preventive measures.Materials and methods. A retrospective analysis of the epidemiological situation on TBVE in the Buryat Republic was carried out using forms of federal statistical surveillance No. 2 “Information on infectious and parasitic diseases” over 2010–2020 and the data from the Reference Center of the Irkutsk Research Anti-Plague Institute of Siberia and Far East of the Rospotrebnadzor on the epidemiological situation and preventive measures in the municipalities of the constituent entity. Statistical processing was performed applying conventional methods of variation statistics. Based on calculated 95 % parametric confidence interval for the data on variability of the long-term average TBVE incidence in the municipalities of the Republic of Buryatia over a 10-year period, the entities were differentiated by epidemiological risk groups. QGis 2.18.28 and a set of open geodata OpenStreetMap were used for mapping.Results and discussion. All municipalities have been classified into five groups by the level of epidemiological risk: with zero TBVE incidence – 2 districts, with a low level – 4, medium – 8, high – 5, very high – 2. In addition, the administrative center has been placed into a separate group. Each individual group of municipalities is characterized by the number of TBVE cases, the level of morbidity, the frequency of seeking medical aid because of tick bites, the scope of specific and non-specific prevention measures. Recommendations for optimizing the tactics of TBVE prevention in certain municipal districts have been provided.
Exposure of Food Samples to Pulsed Microwave Radiation to Increase their Microbiological Safety and Shelf Life
Authors: Gulyaev Yu. V. , Meshchanov V. P. , Kats B. M. , Koplevatsky N. A. , Lopatin A. A. , Sayapin K. A. , Elkin V. A. , Komarov V. V. , Bayburin V. B. , Rytik A. P. 
Published: November 2022

The aim of the study was to increase the efficiency of decontamination of biological material and media (by the example of food products) by pulsed (non-thermal) radio emission and asses the prospects of its application in medicine and biology.Materials and methods. To achieve the goal an experimental setup has been designed, manufactured and tested, which makes it possible to study the process of exposure of biological materials and media to pulsed (non-thermal) radio emission, in particular, by the example of food products. The basis of the method is optimum control of the electro-physical parameters of the irradiating radio signal, depending on the type of the irradiated object. We used pulsed magnetrons with operating frequency of (2.45±0.05) GHz, authorized for bio-medical research, generating pulsed radiation with an adjustable power within the range of 0.1...10 kW. The pulse repetition rate with a duty cycle of 500...10000 is 0.1...5 kHz. The setup has an operating chamber into which the test sample is placed, as well as additional elements of magnetron protection and measuring the parameters of the microwave power incident on biological object.Results and discussion. The setup has been successfully used to irradiate various food samples with pathogenic micro flora (Salmonella spp. etc.) with pulsed microwave radiation. In particular, as shown by the studies, the arithmetic mean number of pathogenic bacteria in the irradiated samples of minced meat decreased by 27.5 times after 28 days of storage as compared to the control group of non-irradiated samples. Preliminary conducted experiments in the field of investigating the effect of microwave radiation on the process of cell division and other aspects of electromagnetic field influence on pathological microorganisms confirm the prospects and the expediency of continuing the ongoing studies in medicine and biology.
Epizooty of Tularemia, Detected in the Population of the Common Vole in the Natural Focus of Steppe Type in the South-East of the Rostov Region in 2020
Authors: Zabashta M. V. , Pichurina N. L. , Khametova A. P. , Zabashta A. V. , Orekhov I. V. , Dobrovol’sky O. P. , Stakheev V. V. , Fomina E. S. , Kovalev E. V. , Fedchenko A. V. , Noskov A. K. 
Published: November 2022

The aim of study was to conduct epizootiological monitoring of natural tularemia foci of the steppe type and investigate epizootic activity in the south-east of the Rostov Region.Materials and methods. An epizootiological survey was carried out on the territory of Remontnensky, Sal’sky and Peschanokopsky districts of the Rostov Region in 2019–2021. To capture and collect mammals, Ixodidae ticks and to study the samples of field material conventional methods were used.Results and discussion. Habitation of 16 species of small mammals, 6 species of Ixodidae ticks has been found. Molecular-genetic analysis of the voles has revealed the presence of the species Microtus arvalis obscurus in the studied area of the region. The circulation of the tularemia agent has been established in the population of common and social voles, forest mouse, hare, rook, Dermacentor marginatus, Hyalomma marginatum, removed from rooks. In May 2020, a high increase in the number of the social vole in the Remontnensky district (up to 21 %), in July 2020 – the common vole in the agrocenoses of the Sal’sky district (up to 33 %) was observed. An extensive epizooty of tularemia was detected in the population of common vole in the south-east of the Rostov Region and in adjacent territories in the Republic of Kalmykia and the Stavropol territory. Two cultures of the pathogen were isolated from the fallen and captured social voles, and four cultures – from the common vole. The isolated strains belong to the Holarctic subspecies of Francisella tularensis EryR. The results obtained attest to the activation of the natural tularemia focus in the south-east of the Rostov Region and its high epizootic activity.