bluuebunny/crossref_metadata_2025_split huggingface dataset:
Broad misappropriation of developmental splicing profile by cancer in multiple organs
Authors: Singh Arashdeep , Rajeevan Arati , Gopalan Vishaka , Agrawal Piyush , Day Chi-Ping , Hannenhalli Sridhar 
Published: December 2022

AbstractOncogenesis mimics key aspects of embryonic development. However, the underlying mechanisms are incompletely understood. Here, we demonstrate that the splicing events specifically active during human organogenesis, are broadly reactivated in the organ-specific tumor. Such events are associated with key oncogenic processes and predict proliferation rates in cancer cell lines as well as patient survival. Such events preferentially target nitrosylation and transmembrane-region domains, whose coordinated splicing in multiple genes respectively affect intracellular transport and N-linked glycosylation. We infer critical splicing factors potentially regulating embryonic splicing events and show that such factors are potential oncogenic drivers and are upregulated specifically in malignant cells. Multiple complementary analyses point to MYC and FOXM1 as potential transcriptional regulators of critical splicing factors in brain and liver. Our study provides a comprehensive demonstration of a splicing-mediated link between development and cancer, and suggest anti-cancer targets including splicing events, and their upstream splicing and transcriptional regulators.
Charge-transfer interface of insulating metal-organic frameworks with metallic conduction
Authors: Sindhu Pooja , Ananthram K. S. , Jain Anil , Tarafder Kartick , Ballav Nirmalya 
Published: December 2022

AbstractDownsizing materials into hetero-structured thin film configurations is an important avenue to capture various interfacial phenomena. Metallic conduction at the interfaces of insulating transition metal oxides and organic molecules are notable examples, though, it remained elusive in the domain of coordination polymers including metal-organic frameworks (MOFs). MOFs are comprised of metal centers connected to organic linkers with an extended coordination geometry and potential void space. Poor orbitals overlap often makes these crystalline solids electrical insulators. Herein, we have fabricated hetero-structured thin film of a Mott and a band insulating MOFs via layer-by-layer method. Electrical transport measurements across the thin film evidenced an interfacial metallic conduction. The origin of such an unusual observation was understood by the first-principles density functional theory calculations; specifically, Bader charge analysis revealed significant accumulation and percolation of charge across the interface. We anticipate similar interfacial effects in other rationally designed hetero-structured thin films of MOFs.
An optimized antimicrobial peptide analog acts as an antibiotic adjuvant to reverse methicillin-resistant Staphylococcus aureus
Authors: Chen Xuan , Wu Xiaoping , Wang Shaoyun 
Published: December 2022

AbstractThe misuse of antibiotics in animal protein production has driven the emergence of a range of drug-resistant pathogens, which threaten existing public health security. Consequently, there is an urgent need to develop novel antimicrobials and new infection treatment options to address the challenges posed by the dramatic spread of antibiotic resistance. Piscidins, a class of fish-specific antimicrobial peptides (AMPs), are regarded as promising therapies for biomedical applications. Progress towards potential analogs from the piscidin family has been hampered by unenforceable structural optimization strategies. Here, we leverage a strategy of bioinformatics analysis combined with molecular dynamics (MD) simulation to identify specific functional hotspots in piscidins and rationally design related analogues. As expected, this approach yields a potent and non-toxic PIS-A-1 that can be used as an antibiotic adjuvant to reverse methicillin-resistant Staphylococcus aureus (MRSA) pathogens. Remarkably, the structural optimization scheme and application strategy proposed here will contribute richer therapeutic options for the safe production of animal protein.
Establishment of a meta-analysis based novel aortic dissection mouse model
Authors: Jiang Hongcheng , Liu Wanjun , He Xingwei , Zeng Hesong 
Published: December 2022

AbstractAortic dissection (AD) is a life-threatening disease and the detailed mechanism remains unclear. Thus, proper animal models are urgently required to better understand its pathogenesis. Our current study aims to establish a reliable, time and cost-effective mouse AD model. To conduct the meta-analysis, we searched PubMed for related studies up to 2021 and statistical analysis was conducted using Review Manager 5.4. For the animal experiment, 6-week-old male ApoE−/− mice were given β-aminopropionitrile (BAPN) at a concentration of 1 g/L for 3 weeks before being infused with saline, 1000 ng/kg/min or 2500 ng/kg/min angiotensin II (AngII) via osmotic mini pumps for 2 or 4 weeks. To determine the presence of AD, we performed B-ultrasonography, hematoxylin and eosin (H&E) staining, and van Gieson staining. The result of the meta-analysis showed that the use of BAPN and more than 2000 ng/kg/min AngII can increase the rate of AD formation, whereas administrating Ang II for more than 28 days has no significant effect on the rate of AD formation when compared with the less than 14 days group. In the present study, mice treated with BAPN combined with 2500 ng/kg/min AngII for 2 weeks (12/20) had a significantly higher AD formation rate than mice treated with BAPN combined with 1000 ng/kg/min Ang II for 4 weeks (2/10), and had a similar model formation rate compared with the mice treated withβ-aminopropionitrile combined with 2500 ng/kg/min AngII for 4 weeks (6/10). There were 3 mice (3/10) and 6 mice (6/20) who died in the group treated with β-aminopropionitrile combined with 2500 ng/kg/min AngII for 4 weeks and 2 weeks respectively, and only one mouse (1/10) died in the group treated with β-aminopropionitrile combined with 1000 ng/kg/min AngII for 4 weeks. In 6-week-old male ApoE−/− mice that received with 1 g/L BAPN in the drinking water for 3 weeks along with 2500 ng/kg/min AngII infusion via osmotic mini pumps for 2 weeks, the highest model formation rate and relative lower cumulative mortality were noted.
Text-based automatic personality prediction using KGrAt-Net: a knowledge graph attention network classifier
Authors: Ramezani Majid , Feizi-Derakhshi Mohammad-Reza , Balafar Mohammad-Ali 
Published: December 2022

AbstractNowadays, a tremendous amount of human communications occur on Internet-based communication infrastructures, like social networks, email, forums, organizational communication platforms, etc. Indeed, the automatic prediction or assessment of individuals’ personalities through their written or exchanged text would be advantageous to ameliorate their relationships. To this end, this paper aims to propose KGrAt-Net, which is a Knowledge Graph Attention Network text classifier. For the first time, it applies the knowledge graph attention network to perform Automatic Personality Prediction (APP), according to the Big Five personality traits. After performing some preprocessing activities, it first tries to acquire a knowing-full representation of the knowledge behind the concepts in the input text by building its equivalent knowledge graph. A knowledge graph collects interlinked descriptions of concepts, entities, and relationships in a machine-readable form. Practically, it provides a machine-readable cognitive understanding of concepts and semantic relationships among them. Then, applying the attention mechanism, it attempts to pay attention to the most relevant parts of the graph to predict the personality traits of the input text. We used 2467 essays from the Essays Dataset. The results demonstrated that KGrAt-Net considerably improved personality prediction accuracies (up to 70.26% on average). Furthermore, KGrAt-Net also uses knowledge graph embedding to enrich the classification, which makes it even more accurate (on average, 72.41%) in APP.
Galleria mellonella immune melanization is fungicidal during infection
Authors: Smith Daniel F. Q. , Dragotakes Quigly , Kulkarni Madhura , Hardwick J. Marie , Casadevall Arturo 
Published: December 2022

AbstractA key component of the insect immune response is melanin production, including within nodules, or aggregations of immune cells surrounding microbes. Melanization produces oxidative and toxic intermediates that limit microbial infections. However, a direct fungicidal role of melanin during infection has not been demonstrated. We previously reported that the fungusCryptococcus neoformansis encapsulated with melanin within nodules ofGalleria mellonellahosts. Here we developed techniques to study melanin’s role duringC. neoformansinfection inG. mellonella. We provided evidence that in vivo melanin-encapsulation was fungicidal. To further study immune melanization, we applied tissue-clearing techniques to visualize melanized nodules in situ throughout the larvae. Further, we developed a time-lapse microscopy protocol to visualize the melanization kinetics in extracted hemolymph following fungal exposure. Using this technique, we found that cryptococcal melanin and laccase enhance immune melanization. We extended this approach to study the fungal pathogensCandida albicansandCandida auris. We find that the yeast morphologies of these fungi elicited robust melanization responses, while hyphal and pseudohyphal morphologies were melanin-evasive. Approximately 23% of melanin-encapsulatedC. albicansyeast can survive and breakthrough the encapsulation. Overall, our results provide direct evidence that immune melanization functions as a direct antifungal mechanism inG. mellonella.
Topologically protected vortex knots and links
Authors: Annala Toni , Zamora-Zamora Roberto , Möttönen Mikko 
Published: December 2022

AbstractIn 1869, Lord Kelvin found that the way vortices are knotted and linked in an ideal fluid remains unchanged in evolution, and consequently hypothesized atoms to be knotted vortices in a ubiquitous ether, different knotting types corresponding to different types of atoms. Even though Kelvin’s atomic theory turned out incorrect, it inspired several important developments, such as the mathematical theory of knots and the investigation of knotted structures that naturally arise in physics. However, in previous studies, knotted and linked structures have been found to untie via local cut-and-paste events referred to as reconnections. Here, in contrast, we construct knots and links of non-Abelian vortices that are topologically protected in the sense that they cannot be dissolved employing local reconnections and strand crossings. Importantly, the topologically protected links are supported by a variety of physical systems such as dilute Bose-Einstein condensates and liquid crystals. We also propose a classification scheme for topological vortex links, in which two structures are considered equivalent if they differ from each other by a sequence of topologically allowed reconnections and strand crossings, in addition to the typical continuous transformations. Interestingly, this scheme produces a remarkably simple classification.
Balint – Psychodrama – Eine Einführung
Authors: Nagel-Brotzler Almut , Bachmann Jean-Pierre 
Published: December 2022

ZusammenfassungIn Balint-Psychodrama-Gruppen treffen sich ÄrztInnen, PsychologInnen und
Angehörige anderer medizinischer Berufsgruppen in Kleingruppen unter der
Leitung von ein oder zwei in der Methode ausgebildeten GruppenleiterInnen
(PsychoanalytikerInnen, psychodynamisch ausgebildete KollegInnen).Das Balint-Psychodrama unterscheidet sich weder im Geist, in den Zielen, noch in
den Ergebnissen von einer anderen Balintgruppe. Der Unterschied liegt im
Wesentlichen in der Art, wie der Fall bearbeitet wird: die Gruppenleitung bittet
den oder die Fallvorstellende/-n (Referenten/-in), nicht nur
über die Begegnung zu berichten, sondern zusammen mit der Gruppe und dem
Gruppenleiter wichtige Momente seiner oder ihrer Beziehung zum Patienten zu
finden, diese in Szene zu setzen und sie mit Hilfe des psychodramatischen Spiels
wieder zu erleben. Das psychodramatische Spiel stellt nur einen Aspekt der
Arbeit dar; vor und nach jeder Spielszene ähnelt die Gruppenarbeit in
Bezug auf den Referenten und die Gruppe jeder anderen Balintgruppe.
Outcomes after Anatomic Double-Bundle Posterior Cruciate Ligament Reconstructions Using Transtibial and Tibial Inlay Techniques
Authors: Temperato Joseph , Rucinski Kylee , Cook James L. , Meers Aaron , Albuquerque João Bourbon de , Stannard James P. 
Published: December 2022

AbstractSurgical reconstruction is recommended for symptomatic posterior cruciate ligament (PCL) deficiency. While anatomic double-bundle PCL reconstruction (PCLR) has been reported to be associated with biomechanical and clinical advantages over other methods, there is still debate regarding the optimal technique for tibial positioning and fixation. Based on reported advantages and disadvantages, we employed two tibial fixation techniques, transtibial (TT) and tibial inlay (TI) for anatomic double-bundle PCLR with technique selection based on body mass index, comorbidities, and primary versus revision surgery. This study aimed to compare clinical outcomes following PCLR utilizing either TT or TI techniques to validate relative advantages, disadvantages, and indications for each based on the review of prospectively collected registry data. For 37 patients meeting inclusion criteria, 26 underwent arthroscopic TT PCLR using all-soft- tissue allograft with suspensory fixation in the tibia and 11 patients underwent open TI PCLR using an allograft with calcaneal bone block and screw fixation in the tibia. There were no significant preoperative differences between cohorts. Success rates were 96% for TT and 91% for TI with all successful cases documented to be associated with good-to-excellent posterior stability and range of motion in the knee at the final follow-up. In addition, patient-reported outcome scores were within clinically meaningful ranges for pain, function, and mental health after PCLR in both cohorts, suggesting similarly favorable functional, social, and psychological outcomes. Patient-reported pain scores at 6 months postoperatively were significantly (p = 0.042) lower in the TT cohort, which was the only statistically significant difference in outcomes noted. The results of this study support the use of TT and TI techniques for double-bundle anatomic PCLR in restoring knee stability and patient function when used for the treatment of isolated and multiligamentous PCL injuries. The choice between tibial fixation methods for PCLR can be appropriately based on patient and injury characteristics that optimize respective advantages for each technique.
Gene variants previously associated with reduced soft‐tissue injury risk: Part 2 – Polygenic associations with elite status in Rugby
Authors: Brazier Jon , Antrobus Mark R. , Herbert Adam J. , Callus Peter C. , Khanal Praval , Stebbings Georgina K. , Day Stephen H. , Heffernan Shane M. , Kilduff Liam P. , Bennett Mark A. , Erskine Robert M. , Raleigh Stuart M. , Collins Malcolm , Pitsiladis Yannis P. , Williams Alun G. 
Published: December 2022

ABSTRACTPart 1 of this genetic association series highlighted several genetic variants independently associated with elite status in rugby. However, it is highly likely that the genetic influence on elite status is polygenic due to the interaction of multiple genes. Therefore, the aim of the present study was to investigate whether polygenic profiles of elite rugby athletes differed from non‐athletes utilising 13 genetic polymorphisms previously associated with tendon/ligament injury. Total genotype score (TGS) was calculated and multifactor dimensionality reduction (MDR) was used to calculate SNP‐SNP epistasis interactions. Based on our elite rugby data from Part 1, mean TGS was significantly higher in elite rugby athletes (52.1 ± 10.7) than non‐athletes (48.7 ± 10.8). There were more elite rugby athletes (54%) within the upper TGS quartile, and fewer (46%) within the lower quartile, compared to non‐athletes (31% and 69%, respectively; P = 5·10−5), and the TGS was able to distinguish between elite rugby athletes and non‐athletes (area under the curve = 0.59; 95% confidence interval 0.55–0.63; P = 9·10−7). Furthermore, MDR identified a three‐SNP model of COL5A1 rs12722, COL5A1 rs3196378 and MIR608 rs4919510 that was best able to predict elite athlete status, with a greater frequency of the CC‐CC‐CC genotype combination in elite rugby athletes (9.8%) than non‐athletes (5.3%). We propose that elite rugby athletes possess “preferable” musculoskeletal soft‐tissue injury‐associated polygenic profiles that have helped them achieve success in the high injury risk environment of rugby. These data may, in future, have implications for the individual management of musculoskeletal soft‐tissue injury.
Spherically symmetric counter examples to the Penrose inequality and the positive mass theorem under the assumption of the weak energy condition
Authors: Jaracz Jaroslaw S 
Published: December 2022

Abstract
Of the various energy conditions which can be assumed when studying mathematical general relativity, intuitively the simplest is the weak energy condition

μ
⩾
0

which simply states that the observed mass-energy density must be non-negative. This energy condition has not received as much attention as the so-called dominant energy condition. When the natural question of the Penrose inequality in the context of the weak energy condition arose, we could not find any results in the literature, and it was not immediately clear whether the inequality would hold, even in spherical symmetry. This led us to constructing a spherically symmetric asymptotically flat initial data set satisfying the weak energy condition which violates the ‘usual’ formulations of the Penrose conjecture. This does not contradict (Malec and Murchadha 1994 Phys. Rev. D
49 6931–4), as there the data is assumed to be maximal. Our result is unexpected and interesting because the heuristic argument behind the Penrose inequality relies on the black hole area law which holds when the weak energy condition is satisfied. Our methods also lead to a counter example to the positive mass theorem assuming the weak energy condition.
Production of runaway electrons and x-rays during streamer inception phase
Authors: Contreras-Vidal Luis , Silva Caitano L da , Sonnenfeld Richard G 
Published: December 2022

Abstract
Streamers play a key role in the formation and propagation of lightning channels. In nature streamers rarely appear alone. Their ensemble behavior is very complex and challenging to describe. For instance, the intricate dynamics within the streamer zone of negative lightning leaders give rise to space stems, which help advance the stepped-leader. Another example is how the increasing morphological complexity of sprites can lead to higher sprite current and greater energy deposition in the mesosphere. Insights into the complex dynamics of a streamer corona can be obtained from laboratory experiments that allow us to control the conditions of streamer formation. Based on simultaneous nanosecond-temporal-resolution photography, and measurements of voltage, current, and x-ray emissions, we report the characteristics of negative laboratory streamers in 88 kPa of atmosphere. The streamers are produced at peak voltages of 62.2 ± 3.8 kV in a point-to-plane discharge gap of 6 cm. While all discharges were driven to the same peak voltage, the discharges occurred at different stages of the relatively slow voltage rise (177 ns), allowing us to study discharge properties as a function of onset voltage. The onset voltage ranged between 24 and 67 kV, but x-ray emissions were observed to only occur above 53 kV, with x-ray burst energies scaling quadratically with voltage. The average delay between the current pulse and x-ray emission was found to be 3.5 ± 0.5 ns, indicating that runaway electrons are produced during the streamer inception phase or no later than the transition stage, when the inception cloud is breaking into streamer filaments. During this short time span, runaway electrons can traverse the gap, hit the ground plate and produce bremsstrahlung x-ray photons. However, streamers themselves cannot traverse more than 3.5 mm across the gap, which supports the idea that runaway electron production is not associated to streamer connection to the ground electrode.
Research on surface roughness prediction in turning Inconel 718 based on Gaussian process regression
Authors: Hao Zhaopeng , Cheng Gang , Fan Yihang 
Published: December 2022

Abstract
Nickel-based alloy Inconel 718 is widely used in aircraft engine industry because of its good mechanical properties. Inconel 718 is a typical difficult-to-machine material and its price is relatively expensive. Therefore, accurate prediction of Inconel 718 machined surface roughness with small sample space can improve machining efficiency, optimize process parameters and reduce machining cost. In this paper, a method is proposed to characterize the influence of cutting parameters on roughness by stablishing the corresponding relationship between the proportional hyperparameters in the multivariate kernel function and the cutting speed, cutting deep, feed rate and the rake angle of the tool. A multi input single output (MISO) multivariate Gaussian process regression (GPR) surface roughness prediction model with cutting speed, cutting depth, feed rate and tool rake angle as input variables and surface roughness as output variables is established. The model can not only output the predicted value of surface roughness, but also give the reliability of the predicted value. Experimental results show that the proportional hyperparameter has an independent adjustment function, and the influence of the process parameters characterized by the proportional hyperparameter on the surface roughness is consistent with the experimental results. The experimental results show that the average relative error of MISO multivariate GPR surface roughness prediction model proposed in this paper is 1.5%, which can accurately predict the surface roughness in small sample space.
The flowering of chrysanthemum (Chrysanthemum sp) growing under various concentrations of liquid organic fertilizer
Authors: Yusuf R. , Syakur A. , Kalaba Y. , Rostiati R. , Ramadani S’ 
Published: December 2022

Abstract
Chrysanthemum is a mainstay commodity in the horticultural industry with bright market prospects. The chrysanthemum plant is one of the horticultural commodities in great demand because it has a variety of colors and shapes; besides that, ornamental plants help beautify the environment. The purpose of this study was to determine the effect of several concentrations of liquid organic fertilizer on the growth and flowering of chrysanthemum plants. This research was conducted at the Screen House of the Faculty of Agriculture, Tadulako University, Palu, Central Sulawesi. This study was arranged using a Randomized Block Design (RBD) consisting of 5 treatments, and each treatment was repeated three times so that there were 15 units of trials. The data obtained were analyzed using ANOVA analysis. The results showed that the liquid organic fertilizer (LOF) of 80 mL/1 liters of water significantly affected the vegetative phase, namely the number of leaves, plant height, and branches. The flowering has no significant effect on the number of flowers, flower diameter, and the time to flower blooming.