bluuebunny/crossref_metadata_2025_split huggingface dataset:
D2d Self-Organization in IOT Via Triple Modular Redundancy Based MDS Code
Authors: Ramamoorthi S , B Muthu Kumar. 
Published: October 2022

Abstract
Device-to-Device (D2D) communication is a key enabling technology to replace base station-to-device(B2D) communication. D2D with IoT realization has spectral and energy-efficient features for on-time quality data transfer. However, the rapid growth of such networks and the dynamic quality of service demands of user equipment require robust and efficient service quality in disasters, outages, and adversarial conditions. To address this challenge, this paper proposes a triple modular redundancy based on the maximum distance separable (TMR-MDS) technique has been proposed, which modifies the repair bandwidth by utilizing more storage occupation and improving the D2D link availability in the system. The proposed TMR-MDS technique will be used, which is composed of two phases: trust management modules and triple modular redundancy storage modules. As part of the initial stage of trust management, a fuzzy logic model is employed to determine the user's trust value after the user's behavior is examined. Second, using the majority voting scheme to improve triple modular redundancy storage. Furthermore, if any problem occurs in the node, then it will self-organize. MATLAB is used to evaluate the performance of the proposed system. The overall system performance is evaluated in terms of the network’s trust level, and the communication cost of the proposed strategy. As per the experimental results, the proposed TMR-MDS method has a better performance of 12.67%, 23.68%, and 25.87% than HBP [4], DR-MDS [5], and URL-RFC [6].
Antifungal activities of vineyard-habitat wild yeast for grape gray-mold disease and its initial promotion of alcohol fermentation in spontaneous winemaking
Authors: Hudagula Hu , Maeno Naoko , Minami Noriko , Takahashi Soichiro , Yoshida Kuniko , Ohtani Katsuki , Funatsu Yasuhiro , Kondo Ryosuke , Yamaguchi Akihiro 
Published: October 2022

Abstract
Microorganisms, including native yeasts, are abundant in vineyard fields. Herein, we studied the possibility of using vineyard-derived wild yeast as a microbial pesticide against Botrytis cinerea, a pathogen that causes grape gray mold disease, to boost the initial alcohol production of spontaneously fermented wine. We identified the Saccharomyces cerevisiae strain KONDO170908, which showed the most effective antifungal activity in an ex vivo yeast dripping experiment on grape berries. This strain was utilized in an in vivo spray test on grape bunches in vineyard fields and was proven to significantly suppress gray mold disease on the grape berries in test plot #16 when the yeast was sprayed during both the flowering and ripening periods (morbidity 11.2% against 15.3% of the control plot, χ2 test, P < 0.0001). However, in test plot #17, spraying the yeast during only the ripening period had no effect (morbidity 16.3%). The grapes from each test plot were also submitted for spontaneous wine fermentation. Alcoholic fermentation of the grapes from test plot #16 provided the most active bubbling of CO2 gas and the highest ethanol production and colony counts over seven days of fermentation. Unique changes in the different strains of S. cerevisiae among the plots were observed throughout the early fermentation stage. Thus, yeast spraying during the flowering period might trigger modification of the entire microbiota and could ultimately contribute to promoting alcohol production in the spontaneously fermented wine.
An Algorithm for Real-Time Sub-Nanosecond Level Time Synchronization Based on RTK Timing Inverse Correction and Kalman Filtering
Authors: Fang Yunlong 
Published: October 2022

Abstract
This article proposes a new algorithm for real-time sub-nanosecond level time synchronization based on GNSS RTK technique and the Kalman Filtering. It is able to improve the performance of time synchronization system as well as a sub-nanosecond level, with a real time response. Although the IGS products theoretically improve the accuracy up to a sub-nanosecond or even higher level, the weakness of more pool performance in a shorter duration and of a lower update rate make us scarcely possibly get an accurate timekeeping upper than nanosecond level only relying on the IGS products. The RTK TIC algorithm uses the RTK position results of (xrtk, yrtk, zrtk) to inversely and iteratively update the time keeping results from pseudorange PVT equations to a high level accuracy. Meanwhile, in order to suppress the error coming from ephemeris change, the FS725 Stanford Rubidium Atomic Clock are used as a compensation equipment for coupling the GNSS timekeeping results via the Kalman Filtering. Then, this paper gives the validation of the RTK TIC algorithm with a two-channel system and compares mean values and standard deviations between our system and two SYN4505As, the results shows our system has a better timekeeping accuracy than the SYN4505As. At last, this paper calibratesthe accuracy of our system via the standard clock source in the NTCS, and gives the results that our system has an excellent performance on the real-time sub-nanosecond level time synchronization.
Predictive value of noninvasive peripheral atherosclerosis measurement for coronary artery disease in patients with long T2DM duration
Authors: Chen Wanjiao , Wang Li , Hu Bin , Zheng Ying , Zhang Shuya , Zhou Zhong , Mai Yifeng 
Published: October 2022

Abstract
Background
Vascular ultrasound is a commonly used tool to predict the existence and severity of coronary artery disease (CAD) by detecting the atherosclerosis of peripheral arteries. However, most studies on atherosclerosis are based on the general population, and the predictive efficacy of carotid or femoral ultrasound in type 2 Diabetes mellitus (T2DM) and its correlation with CAD severity are less elucidated. This study aimed to compare the predictive value of carotid or femoral artery ultrasound for CAD in T2DM patients free from known CAD, and to assess the relationship with the severity of coronary artery stenosis.
Methods
Cross-sectional study in adults with a T2DM duration of at least 5 years and without established CAD. All participants underwent carotid and femoral ultrasound and underwent coronary angiography for the first time. The carotid plaque score (CPS) was calculated by the sum of the maximum thickness of plaques on three segments of the carotid arteries on both sides. The Gensini score was used to assess the severity of coronary stenosis, and patients were divided into no or mild group, moderate group and severe group according to the tertile of the score.
Results
Multiple regression analysis showed that carotid plaque score, femoral plaque and smoking were independently and positively correlated with CAD. Carotid plaque score yielded the highest area under the curve for detecting significant coronary disease (AUC = 0.7323). In contrast, the area under the curve of femoral artery plaque and carotid intima-media thickness was lower than 0.7, which was at a lower prediction level. In the no or mild, moderate and severe groups, the CPS value gradually increased, and the difference between the groups was statistically significant (p < 0.05). The positive rate of femoral artery plaque in patients with moderate and severe CAD was significantly higher than that in the no or mild group (p < 0.0001).
Conclusion
In patients with long T2DM duration, carotid plaque score has a higher ability to predict the occurrence and severity of CAD. However, femoral artery plaque has special value in predicting moderate to severe coronary artery disease in patients with long-term T2DM.
Randomized in situ evaluation of surface polishing protocols on the caries-protective effect of resin Infiltrant
Authors: Esteves-Oliveira Marcella , Passos Vanara Florencio , Russi Tereza M.A.Z.C. , Fernandes Argus R. R. , Terto Caroline N.N. , Mendonça Juliano S. , Campus Guglielmo , Wierichs Richard J. , Meyer-Lückel Hendrik , Lima Juliana P.M. 
Published: October 2022

Abstract
The aim of this placebo-controlled randomized in situ study was to evaluate the effect of different surface polishing protocols on enamel roughness, bacterial adhesion and caries-protective effect of a resin infiltrant. Eighty-four bovine enamel samples having artificial caries lesions were treated with a resinous infiltrant and afterwards randomly dividided into five polishing protocols: aluminum oxide flexible disks (Al2O3-Disks), silicon carbide tips (SIC-Tips), silicon carbide brush (SIC-Brush), silicon carbide polyester strips (SIC-Strips) or no polishing [negative control (NC)]. Average surface roughness (Ra) was assessed by profilometry. Samples were mounted in palatal appliances under a mesh for biofilm accumulation. Fifteen volunteers wore the intraoral appliances (14-days) and cariogenic challenge was triggered by sucrose solutions. Biofilm formed was collected for microbiological analysis of caries-related bacteria (Streptococcus mutans, Lactobacillus acidophilus) and demineralization was assessed by cross-sectional microhardness. Mean Knoop hardness numbers (Kg/mm2) were plotted over lesion depth (µm) and area under the lesion curve was subtracted from sound enamel to determine demineralization (ΔS, Kg/mm2xµm). Data were analyzed by ANOVA and post-hoc comparisons (α=0.05). NC resulted in significantly higher Ra means than Al2O3-Disks and SIC-Strips. Bacterial counts were not significantly different between the groups (p>0.05). Regards ΔS means, however none of the groups were significantly different to NC (6,983.3 Kg/mm2xµm /CI:4,246.1–9,720.5, p>0.05). Conclusions: Polishing protocols (Al2O3-Disks, SIC-Strips) significantly decreseased roughness of infiltrated-enamel, however none of the polishing protocols could signicantly decrease bacterial counts nor resulted in significant less demineralization.
Surgical treatment versus non-surgical treatment for Carpal Tunnel Syndrome: A Systematic Review
Authors: Cao Manting , Hu Hantong , Chen Jing 
Published: October 2022

Abstract
Study design: Systematic review.
Introduction: Carpal tunnel syndrome (CTS) is a common and painful compressive peripheral neuropathy with complex symptoms, caused by pressure on the median nerve as it travels through the carpal tunnel.
Purpose of the Study: This review aimed to develop a critical systematic review of the research evidence to evaluate the effectiveness of non-surgical intervention compared to surgical intervention in the treatment of patients with CTS.
Methods: Four electronic databases (PEDro, PubMed/MEDLINE, Cochrane Library, and Web of Science) and two grey literature databases (US ClinicalTrials. gov, and OpenGrey) were used to perform a comprehensive search of the relevant data.
Results: A total of 686 potentially related studies were found in the four databases, and no relevant studies were found in the two gray literature databases. 10 studies finally met the inclusion criteria and were included in this review.Five studies compared the effect of local steroid injection and surgical treatment on patients with CTS. Two studies compared manual therapy with surgical treatment.; One study compared splinting with surgical treatments; One study make a comparison between a combined non-surgical treatment program and non-surgical treatment; One study compared the effectiveness of splinting, splinting combined with local steroid injection and surgical treatment.
Conclusion:In conclusion, critical analysis of the findings shows that both surgical treatment and non-surgical treatment are effective for the management of patients with CTS. Indeed, surgical treatment shows better effectiveness.
Critical analysis of the findings showed that both surgical and non-surgical treatments are effective for the management of patients with CTS. Thus, surgical treatment is more effective.
Clinical significance of mutational variants in beta and alpha genes in patients with hemoglobinopathies from two large greek centres: a complex interplay between genotype and phenotype
Authors: Diamantidis Michael D. , Karanikola Rebecca-Anastasia , Polyzoudi Chrysoula , Delicou Sophia , Manafas Achilles , Savera Helen , Xydaki Aikaterini , Kotsiafti Angeliki , Tsangalas Evangelos , Ikonomou Georgia , Mani Eirini , Ntoulas Konstantinos , Alexiou Evangelos , Koskinas John , Fotiou Paraskevi 
Published: October 2022

Abstract
Hemoglobinopathies affect patients in the wider Mediterranean area and consist of 4 distinct subgroups: beta thalassemia major (TM), beta thalassemia intermedia (TI), sickle cell disease syndromes (SCD) (homozygous SCD, SCD/beta thalassemia trait) and hemoglobinopathy H (alpha thalassemia). The clinical spectrum of these syndromes varies from mild to severe. Complex interactions between genes and environmental factors form the clinical manifestations in hemoglobinopathies. There is an unmet need to clarify these multifactorial mechanisms. This is the first Greek study, describing mutational alleles (variants in the HBB and HBA1/HBA2 genes, type of mutation and prevalence) in 217 patients with hemoglobinopathies of two large centers in Greece (Larissa and Athens) and associating particular genotypes or gene variants with clinical manifestations (transfusion frequency, complications). Thus, the complex interplay between corresponding genotypes and phenotypes was investigated. The present study results are in accordance with previous national studies with limited variations, due to regional prevalence of specific gene variants, as expected. The type and prevalence of variants in beta and alpha globin genes differ significantly among countries. In the beta thalassemic or SCD patients of our cohort, co-inheritance of variants in the alpha globin genes, leading to absence or reduction of alpha globin synthesis were associated with milder clinical course, whereas the inheritance of additional alpha genes (triplication) led to a more severe clinical phenotype. In cases in whom the genotype and phenotype did not correlate, other factors such as the function or modification of possible regulatory genes or additional nutritional or environmental effects should be investigated.
Does joint-sparing tumor resection jeopardize oncologic and functional outcomes in non-metastatic high-grade osteosarcoma around the knee?
Authors: Huang Mengquan , Ma Ziyang , Yu Jie , Lu Yajie , Chen Guojing , Fan Jian , Li Minghui , Ji Chuanlei , Xiao Xin , Li Jing 
Published: October 2022

Abstract
Background:We previously reported joint-sparing tumor resection for osteosarcoma with epiphyseal involvement in which transepiphyseal osteotomy went through the in situ ablated epiphysis. However, we do not know whether this is a safe approach when compared with joint-sacrificed tumor resection. Our objective was to compare oncologic and functional outcomes between patients who underwent joint preservation (JP) and joint replacement (JR) tumor resection. Furthermore, we identified the risk factors of local recurrence, metastasis and survival.
Methods: Eighty-nine patients with non-metastatic high-grade osteosarcoma around the knee were treated with limb-salvage surgery (JP in 47 and JR in 42). Age, gender, tumor location, pathologic fracture, plain radiographic pattern, limb diameter change, perivascular space alteration, surgical margin, local recurrence, metastasis, death and the Musculoskeletal Tumor Society (MSTS)-93 scores were extracted from the records. Univariate analysis was performed to compare oncologic and functional outcomes. Binary logistic and cox regression models were used to identify predicted factors for local recurrence, metastasis, and survival.
Results:Local recurrence, metastasis and overall survival were similar in the JP and JR group (p =0.3; p=0.211; p=0.143). Major complications and limb survival were also similar in the JR and JP group (p=0.14; p=0.181). The MSTS score of 27.06 ±1.77 in the JP group was higher than that of 25.88±1.79 in the JR group (p=0.005). The marginal margin of soft tissue compared with a wide margin was the only independent predictor of local recurrence (p=0.006). Limb diameter increase and perivascular fat plane disappearance during neoadjuvant chemotherapy were independent predictors for metastasis (p=0.002; p=0.000) and worse survival (p=0.000; p=0.001).
Conclusions: Joint-sparing tumor resection with the ablative bone margin offers advantage of native joint preservation with favorable functional outcomes while not jeopardizing oncologic outcomes compared with joint-sacrificed tumor resection. Surgeon should strive to obtain adequate soft tissue surgical margin decreasing risk of local recurrence. Novel drug regimens might be reasonable options for patients with obvious limb diameter increase and perivascular fat disappearance during chemotherapy.
Genome-wide analysis of histone trimethylation reveals a global impact of bisphenol A on telomeric binding proteins and histone acetyltransferase factors: Complementing in vitro and human data from the INMA cohort.
Authors: D’Cruz Shereen Cynthia , Hao Chunxiang , Labussiere Martin , Mustieles Vicente , Freire Carmen , Legoff Louis , Magnaghi-Jaulin Laura , Olivas-Martinez Alicia , Rodriguez-Carrillo Andrea , Jaulin Christian , David Arthur , Fernández Mariana F. , Smagulova Fatima 
Published: October 2022

Abstract
Objective: To assess the genetic and epigenetic effects promoted by Bisphenol A(BPA) exposure in adolescent males from the Spanish INMA-Granada birth cohort, as well as in human cells.
Methods: DNA methylation was analysed using MEDIP. Repeat number variation in genomic DNA was evaluated, along with the analysis of H3K4me3 by using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). All experiments were performed with material extracted from whole blood of adolescents from INMA. The epidemiological study was complemented by in vitro assessments of human (HeLa) cells exposed to BPA, specifically, immunofluorescence evaluation of histone modification levels, gene expression analysis and ChIP‒qPCR analysis.
Results: Adolescents in the high urinary BPA group presented higher genetic instability of Satellite A (SATA) repetitive region compared to those in the low BPA group. We also observed decreased DNA methylation at the promoters of the imprinted genes H19, KCNQ1, and IGF2; at LINE1 retroelements; and at the ARID2, EGFR1 and ESRRA genes. Genome-wide sequencing revealed increased H3K4me3 occupancy at the promoters of genes encoding histone acetyltransferases, telomeric DNA binding factors and DNA repair genes. These results were supported by studying HeLa cells exposed to 10 nMBPA in vitro. Exposure of cells to BPA caused a global increase in histone H4 acetylation and a decrease in H3K9me3 levels. In exposed cells, changes in the expression of genes encoding DNA repair factors (ATM, ARID2) were observed, and the expression of several genesencoding telomeric DNA binding factors (SMG7, TERT, TEN1, UPF1, ZBTB48) increased. Moreover, increased binding of ESR1 to KAT5, KMT2E and TERF2IP promoters and decreased ESR1 binding at the RARA promoter were observed.
Conclusion: Genome-wide analysis of histone trimethylation and BPA exposure in the in adolescents from the INMA cohort revealed a global impact of BPA on the expression of genes encoding telomeric binding proteins and histone acetyltransferase factors, which showed parallels with HeLa cells exposed to a human-relevant dose.
FORWARDS-1; An adaptive, single-blind, placebo-controlled ascending dose study of acute baclofen on safety parameters in opioid dependence during methadone-maintenance treatment; a pharmacokinetic-pharmacodynamic study.
Authors: Paterson Louise M , Barker Dominic , Cro Suzie , Mozgunov Pavel , Phillips Rachel , Smith Claire , Nahar Limon K , Paterson Susan , Lingford-Hughes Anne R 
Published: October 2022

Abstract
Background

Treatment of opiate addiction with opiate substitution treatment (e.g. methadone) is beneficial. However some individuals desire or would benefit from abstinence but there are limited options to attenuate problems with opiate withdrawal. Preclinical and preliminary clinical evidence suggests that the GABA-B agonist, baclofen, has the desired properties to facilitate opiate detoxification and prevent relapse. This study aims to understand whether there are any safety issues in administering baclofen to opioid dependent individuals receiving methadone.

Methods

Opiate dependent individuals (DSM-5 severe opioid use disorder) maintained on methadone will be recruited from addiction services in northwest London (NHS and third sector providers). Participants will be medically healthy with no severe chronic obstructive pulmonary disease or Type 2 respiratory failure, no current dependence on other substances (excluding nicotine), no current severe DSM-5 psychiatric disorders, and no contraindications for baclofen or 4800IU vitamin D (placebo). Eligible participants will be randomised in a 3:1 ratio to receive baclofen or placebo in an adaptive, single-blind, ascending dose design. A Bayesian dose-escalation model will inform the baclofen dose (10, 30, 60 or 90mg) based on the incidence of ‘dose-limiting toxicity’ (DLT) events and participant-specific methadone dose. A range of respiratory, cardiovascular and sedative measures including the National Early Warning Score (NEWS2) and Glasgow Coma Scale will determine DLT. On the experimental day, participants will consume their usual daily dose of methadone followed by an acute dose of baclofen or placebo (vitamin D3) ~ 1 hour later. Measures including oxygen saturation, transcutaneous CO2, respiratory rate, QTc interval, subjective effects (sedation, drug liking, craving), plasma levels (baclofen, methadone) and adverse events will be obtained using validated questionnaires and examinations periodically for 5 hours after dosing.

Discussion

Study outcomes will determine what dose of baclofen is safe to prescribe to those receiving methadone, to inform a subsequent proof-of-concept trial of the efficacy baclofen to facilitate opiate detoxification. To proceed, the minimum acceptable dose is 30mg of baclofen in patients receiving ≤ 60mg/d methadone based on clinical experience of baclofen’s use in alcoholism and guidelines for the management of opiate dependence.
Trial registration: Clinicaltrials.gov: NCT05161351, registration date: 16 Dec 2021.
Pre-T cell receptor signaling drives leukemogenesis and is a therapeutic target in T-cell acute lymphoblastic leukemia
Authors: Meter Michael Van , Gao Jie , Wang Wei , Kunz Arthur , Bay Andres Perez , Nittoli Thomas , Thurston Gavin , Kuhnert Frank 
Published: October 2022

AbstractT-cell acute lymphoblastic leukemia (T-ALL) is an aggressive lymphoid neoplasm resulting from the malignant transformation of T-cell progenitors. T-ALL leukemogenesis is strongly influenced by its developmental origins.NOTCH1, a critical regulator of early thymocyte development, is mutated in ~ 60% of T-ALL. Less is known about how other determinants of thymocyte development participate in T-ALL leukemogenesis. Using a thymus transplantation-based model of T-ALL, we found that multiple β-selection checkpoint factors were upregulated in leukemic T cells, including Ptcra, a subunit of the pre-T cell receptor (pre-TCR). Genetic ablation ofPtcradramatically reduced the occurrence of T-ALL in this model. In human T-ALL cell lines, knockout ofPTCRAreduced proliferative capacity and the ability to form tumors in vivo, supporting a driver oncogenic role. T-cell antigen-directed immunotherapies face the potential hurdle of inducing severe immunosuppression due to depletion of normal T cell populations. Analysis of clinical T-ALL samples demonstrated thatPTCRAis highly and specifically expressed in leukemic T cells but not in normal, mature T cells. Finally, we demonstrate that PTCRA-directed antibody-drug conjugates have anti-leukemic activity in vitro and in vivo. Cumulatively, our findings highlight an important role for pre-TCR in driving T-ALL and support further evaluation of PTCRA-directed therapies.