bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and Biochemical Studies of Gamma Irradiated Rates Treated by Mesenchymal Stem Cells and Natural Antioxidant
Authors: Mahmoud Ashraf Z. , El.Sawi M. R. , Habza M. N. 
Published: February 2022

Abstract
Purpose: Therapeutic effect of mesenchymal stem cells transplantation (MSCs) and natural antioxidant such as silymarin have been postulated as hepato-protectors against ionizing radiation induced harm. The present study was undertaken to evaluate the protective effect of (MSCs) and silymarin to ameliorate damage caused by gamma radiation. Materials and methods: Three days before irradiation, rats were given silymarin at a dose of 70 mg/kg dissolved in distilled water and continued for another 21 days. One day after gamma irradiation at a dose level of 4Gy, male rats were transplanted by bone marrow mesenchymal stem cells (BMMSCs) through intravenous injection. Results: One and three weeks after irradiation, irradiated animals receiving transplanted mesenchymal stem cells (TMSCs) and silymarin seperatly or with each other exhibited a pronounced elevation in liver antioxidant such as Glutathione (GSH) superoxide dismutase (SOD), glutathione –S- transferase (GST), total antioxidant capacity (TAC), catalase (CAT) and glucose-6-phosphate dehydrogenase (G-6-PDase) activity accompanied with significantdecline in lipid peroxidation and hydrogen peroxide levels in comparing with irradiated rats. Moreover, RAPD-PCR with primers OP-B10 and OP-B14 exhibited strong and different banding patterns in all treated rats compared to untreated control rats after 1 and 3 weeks of treatment.Conclusion: Treatment with MSCs and silymarin possess a radio protective capacity against ionizing-radiation induced oxidative stress and organ injury.
Oral etoposide and trastuzumab in HER2-positive metastatic breast cancer: a retrospective study at Institut Curie Hospitals.
Authors: Lerebours Florence , Chalumeau Clelia , Carton Matthieu , Eeckhoutte Alexandre , Ballet Stelly , Vincent-Salomon Anne , Vuagnat Perrine , Bellesoeur Audrey , Pierga Jean-Yves , Stern Marc-Henri , Bidard Francois-Clement 
Published: February 2022

Abstract
Background: The TOP2A and ERBB2 genes are co-amplified in about 40% of HER2 positive (HER2+) breast cancers. Oral etoposide (VP16), an inhibitor of topoisomerase-II (encoded by TOP2A), has demonstrated clinical activity in metastatic breast cancer (MBC). However, the clinical benefit of oral VP16 combined with trastuzumab (VP16-T) in HER2+ MBC has not been evaluated.Methods: Patients treated at Institut Curie Hospitals with VP16-T for HER2+ MBC were retrieved by an in-silico search. Trained medical oncologists retrospectively assessed progression-free survival (PFS), overall survival (OS), response rate, prolonged PFS (defined as a duration of at least 6 months), 6 months clinical benefit rate and toxicity. Co-amplification of ERBB2 and TOP2A was assessed by shallow whole genome sequencing on tumor tissue whenever available.Results: Forty-three patients received VP16-T after a median number of six prior treatment lines for HER2+ MBC. Median PFS and OS were 2.9 months (95% CI [2.4-4.7]) and 11.3 months (95% CI [8.3-25.0]), respectively. Three patients had a complete response while 12/40 (30%) had a clinical benefit. Only 3 patients stopped treatment for toxicity. Median PFS in the population with and without TOP2A/ERBB2 co-amplification was respectively 4.7 months (95% CI [2.3-NA]) and 2.9 months (95% CI [1.2-NA]; p=0.36).Conclusion: Our analysis suggests a favorable efficacy and toxicity profile for VP16-T in patients with heavily pretreated HER2+ MBC.
Synecological response of desert spring benthic prokaryotes and macroinvertebrates to Sierra Nevada roof pendant-derived calcium
Authors: Friel Ariel D. , Pordel Khaled , Meyers Zach , Seymour Cale O. , Thomas Nicole J. , Phillips Fred M. , Knott Jeffrey R. , Sada Donald W. , Rademacher Laura , Frisbee Marty , Hedlund Brian P. 
Published: February 2022

Abstract
Despite the ecological significance of desert springs, little is known about relationships between spring hydrogeochemistry and ecology, particularly over multiple trophic levels. Here, we surveyed microbial communities (bacteria and archaea) and benthic macroinvertebrate (BMI) communities in springs that recharge through granitoid rocks in Owens Valley, CA, to determine whether subtle geochemical differences imparted by Paleozoic roof pendant weathering in the source area affects spring ecosystems. Relative to other springs, roof pendant-recharged springs were characterized by elevated: (i) Ca2+/Na+, Ca2+/Mg2+, and divalent/monovalent cation ratios, (ii) relative abundance of benthic aerobes/facultative anaerobes and holdfast/stalked bacteria, and (iii) abundance and diversity of shredder and collector-gatherer BMIs. These BMI feeding groups graze on biofilm communities and stimulate bacterial degradation of particulate organic matter, consistent with extensive bacteria-BMI connections in co-occurrence networks of these springs. Springs not impacted by roof pendants were instead enriched with anaerobes and chemolithotrophs and low-diversity BMI communities with poor bacteria-BMI network connectivity. We speculate that excess Ca2+ derived from roof pendant weathering plays a key role in biofilm formation on coarse granite substrates, with subsequent synergy between benthic microbial biofilms and diverse BMI shredders/collector-gatherers to degrade allochthonous organic material.
Deep brain stimulation re-imagined: An ultra-low frequency spike timing dependent plasticity-based approach for treating alcohol use disorder
Authors: Asp Anders , Boschen Suelen Lucio , Chang Su-Youne , Lujan J. Luis 
Published: February 2022

Abstract
Alcohol use disorder (AUD) is a chronic relapsing brain disorder characterized by an impaired ability to stop or control alcohol consumption despite adverse social, occupational, or health consequences. AUD affects nearly one-third of adults at some point during their lives, with an associated cost of approximately $249 billion annually in the U.S. alone. The effects of alcohol consumption are expected to increase significantly during the COVID-19 pandemic, with alcohol sales increased by approximately 54%, potentially exacerbating health concerns and risk-taking behaviors. Unfortunately, existing pharmacological and behavioral therapies for AUD have historically been associated with poor success rates, with approximately 40% of individuals relapsing within three years of treatment. Pre-clinical studies have shown that chronic alcohol consumption leads to significant changes in synaptic function within the dorsal medial striatum (DMS), one of the brain regions associated with AUD and responsible for mediating goal-directed behavior. Specifically, chronic alcohol consumption has been associated with hyperactivity of dopamine receptor 1 (D1) medium spiny neurons (MSN) and hypoactivity of dopamine receptor 2 (D1) MSNs within the DMS. Optogenetic, chemogenetic, and transgenic approaches have demonstrated that reducing the D1/D2 MSN signaling imbalance decreases alcohol self-administration in rodent models of AUD. Here, we present an electrical stimulation approach that uses ultra-low (≤ 1Hz) frequency (ULF) spike-timing dependent plasticity (STDP) in mouse models of AUD to reduce DMS D1/D2 MSN signaling imbalances by stimulating D1-MSN afferents into the GPi and ACC glutamatergic projections to the DMS in a time-locked stimulation sequence. Our data suggest that GPi/ACC ULF-STDP selectively decreases DMS D1-MSN hyperactivity leading to reduced alcohol consumption without evoking undesired affective behaviors using electrical stimulation rather than approaches requiring genetic modification. This work represents a step towards fulfilling the unmet need for a reliable method of treating severe AUD through cell-type specific control with clinically available neuromodulation tools.
Incidence and non-union rate of tibia fractures in adults with Osteogenesis Imperfecta: a retrospective cohort study of an expert clinic of 402 patients with 42 fractures.
Authors: Munk Simone , Harsevoort Gerrit Jan , Gooijer Koert , Edens Mireille , Franken Antonius , Janus Guus 
Published: February 2022

Abstract
Purpose Tibia fractures are the most common fractures seen in adults and also lead to the most non-unions. Osteogenesis Imperfecta (OI) is characterized by an increased bone fragility and a higher risk of fractures. No previous studies have been published concerning the incidence of tibia fractures and non-unions in adults with OI. This study aims to give an overview of the incidence of tibia fractures and non-unions in adult OI patients. MethodsIt is a retrospective, descriptive study where all medical charts of adult patients in the OI database of our OI expert clinic were analyzed for tibia fractures between 2008 and 2020. Tibia fracture incidence, non-union rate, treatment modality and potential risk factors were determined.ResultsThe database consisted of 402 patients of which 34 patients had suffered one or more tibia fractures, resulting in 42 fractures. The incidence of a tibia fracture in OI adults is 870 per 100.000 person-years. Two out of 42 fractures led to a non-union (5%). It was not possible to adjust for risk factors or type of treatment. ConclusionThere is a higher incidence of tibia fractures in OI patients, but a comparable non-union rate to the general population. With only 2 non-unions it is not possible to draw conclusions on the influence of risk factors or treatment of tibia fractures in OI.
Daily blood pressure profile and blood–brain barrier permeability in patients with cerebral small vessel disease
Authors: Dobrynina L. A. , Shamtieva K. V. , Kremneva E. I. , Zabitova M. R. , Akhmetzyanov B. M. , Gnedovskaya E. V. , Krotenkova M. V. 
Published: February 2022

Abstract
BackgroundCerebral small vessel disease (CSVD) plays an important role in cognitive impairment (CI), stroke, disability and death. Arterial hypertension (AH) is the main risk factor for CSVD. The use of antihypertensive therapy (AHT) has not resulted in the expected decrease in CSVD complications, which may be related to the underestimation of significance of daily blood pressure profile for blood–brain barrier (BBB) permeability. Methods53 patients with CSVD of varying severity (mean age 60.086.8 years, 69.8% women, subjects with long-standing AH vs. normotensive subjects – 84.8% vs. 15.2%) and 17 healthy volunteers underwent ambulatory blood pressure monitoring (ABPM) and MRI, including T1-weighted dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for assessing BBB permeability. ResultsMost of ABPM parameters in CSVD patients did not differ from controls, but were associated with the severity of white matter hyperintensity (WMH) and the total CSVD score. BBB permeability in normal-appearing white matter (NAWM) and grey matter (GM) was significantly higher in CSVD patients, and the severity of BBB permeability remained similar in patients with different stages of WMH. Among BBB permeability parameters, the area under the curve, corresponding to an increase in the contrast transit time in NAWM, had the greatest number of correlations with deviations of ABPM parameters. ConclusionBBB permeability in CSVD is a universal mechanism of NAWM and GM damage associated with a slight increase in ABPM parameters. It is obvious that the treatment of AH in patients with not severe WMH should be more aggressive and carried out under the control of ABPM.
Postoperative hyperprogression disease of pancreatic ductal adenocarcinoma after curative resection: A retrospective cohort study.
Authors: Zou Siyi , Wang Xinjing , Chen Haoda , Lin Jiewei , Wen Chenlei , Zhan Qian , Chen Hao , Lu Xiongxiong , Deng Xiaxing , Shen Baiyong 
Published: February 2022

Abstract
Background: Prognosis for patients recurred rapidly after resection of pancreatic ductal adenocarcinoma (PDAC) was extremely poor. We proposed the concept of postoperative hyper-progression disease (PO-HPD) to define recurrence within 2 months after surgery, explored the role of surgery for postoperative HPD patients and determined the predictive preoperative risk factors and genomic features of PO-HPD. Methods: 976 patients undergoing curative resection of PDAC were enrolled. Survival data of 1733 stage IV patients from the US Surveillance, Epidemiology and End Results database was also collected. Patients relapsed were grouped into 3 groups regarding of the recurrence time (within 2 months were PO-HPD, within 2 to 12 months were early recurrence (ER) and within >12 months were late recurrence (LR)). Risk factors for PO-HPD were explored with logistic regression models. Genomic features of 113 patients were investigated using next-generation sequencing-based gene panel testing. Results: 718 of 976 cases relapsed, 101were PO-HPD, 418 were ER and 199 were LR. Total survival of PO-HPD was 12.5 months, shorter than that of ER (16.7 months) and LR (35.1 months), and verged on that of stage IV patients (10.6 months). Preoperative risk factors for PO-HPD included red blood cell count <3.94*10^12/L, CA19-9≥288.6 U/mL, CA125≥22.3 U/mL and tumor size≥3.45cm. Mutations of CEBPA, ATR and JAK1 were only identified in PO-HPD and they owned lower level of CN gain compared to others. Conclusions: Prognosis of PO-HPD was extremely poor and the role of surgery for PO-HPD should be prudently assessed.
Efficient World-Line-Based Quantum Monte Carlo Method Without Hubbard-Stratonovich Transformation
Authors: Wang J. , Pan W. , Sun D. Y. 
Published: February 2022

Abstract
By precisely writing down the matrix element of the local Boltzmann operator (e-th, where h is the Hermitian conjugate pairs of off-diagonal operators), we have proposed a new path integral formulation for quantum field theory and developed a corresponding Monte Carlo algorithm. With current formula, the Hubbard–Stratonovich transformation is not necessary, and is not based on the determinant approach, which can improve the computational efficiency. The results show that, the simulation time has the square-law scaling with system sizes, which is comparable with the usual first-principle calculation. The current formula also improves the accuracy of the Suzuki–Trotter decomposition. As an example, we have studied the one-dimensional half-filled Hubbard model at finite temperature. The obtained results are in excellent agreement with the known solutions. The new formula and Monte Carlo algorithm can be used in various studies.
Early Assessment of Ventricular Synchronization and Function After Left Bundle-Branch-Area Pacing with Right Bundle-Branch Block
Authors: Zhao Ruohan , Xiong Feng , Deng Xiaoqi , Wang Shuzhen , Liu Chunxia , Xu Min , Tan Kunyue , Wang Xiuxiu 
Published: February 2022

Abstract
AimTo evaluate ventricular synchronization and function in patients with right bundle-branch block after left bundle-branch-area pacing (LBBAP) by echocardiography.MethodsForty patients who successfully received LBBAP were selected and divided into the right bundle-branch block group (RBBB group) and the non-RBBB group by pre-operation ECG. Echocardiography and follow-up were performed 1 month after operation. Interventricular synchronization was evaluated by tissue Doppler (TDI), tissue mitral annular displacement (TMAD), and interventricular mechanical delay (IVMD). The tricuspid annular plane systolic excursion (TAPSE), right ventricular fractional area change(RVFAC), tricuspid annulus sidewall systolic velocity (TV-s’) ,global ventricular longitudinal strain(GLS) ,right ventricular free wall longitudinal strain(LS-RV) , standard deviation of left ventricular 18 segments peak time difference(SDt-L) and standard deviation of right ventricular free wall 3 segments peak time difference(SDt-R) were applied to evaluate intraventricular synchronization and ventricular function.ResultsThe deviation of displacement peak time of the tricuspid and mitral valves, namely ΔPTTV-MV measured by TMAD, the deviation of systolic time to peak of the tricuspid and mitral valves, namely ΔTsTV-MV measured by TDI, were statistically different between the two groups (P < 0.05). (2) Compared with the non-RBBB group, there were no statistically significant differences in the GLS, RVFAC, LS-R,TAPSE, TV-s’, SDt-L, SDt-R (P > 0.05).ConclusionEchocardiography technology including two-dimensional speckle tracking imaging (2D-STI), TDI, and TMAD can effectively analyze interventricular synchronization, intraventricular synchronization, and ventricular function. Although the movement of the right ventricular myocardium in the RBBB group was slightly later than that of the left ventricular myocardium after LBBAP, LBBAP could still be applied in RBBB patients with pacing indication.
Measurement Of Energy Poverty In The Colombian Caribbean Region: Comparative Analysis.
Authors: Bayona-Velásquez Etna , Pirela-Ríos Ana , Alvarez José Ricardo Núñez , Marín-Giraldo Ever 
Published: February 2022

Abstract
This research aims to analyze the level of energy poverty and its consequences on the quality of life of the population of the Colombian Caribbean, comparing the results obtained in this region with the data corresponding to the people of Bogotá, the capital of Colombia, and the rest from the country. Energy poverty is the deprivation of goods and services that meet basic needs for the non-use of electricity, which affects the health, education, and social inclusion of the affected population. This study applied the method of meeting absolute energy needs, determining the Energy Poverty in the Home (PEH) index. The results indicate that energy-poor households exceed 60% in urban areas and 96% in rural areas, linking energy poverty and school dropout.
A Study of Isothermal Mechanical Fatigue of Welded Reactor Steel (15H2MFA)
Authors: Keba Degu Fedesa , Thanganadar Ashokkumar , Abishini A. H. 
Published: February 2022

Abstract
In material science and engineering the fatigue of metal is quite difficult to deal with and that of welded joint is an even more complex phenomenon. This complexity arises from different main factors: thermal cycles during welding, which will strongly affect the base material and induce residual stresses and distortion, the fusion process with the filler metal leads to heterogeneity to microstructure which will affect or change the original mechanical properties and chemical composition through out of the welded joint. In this study isothermal mechanical fatigue of welded reactor steel has been contemplated. The samples of the welded joint have used in this study is 15H2MFA steel type of VVER-440 reactor pressure vessel operating at Paks Nuclear Power Plant, Hungary. The reactor vessel is made of low-alloy heat resistant steel and austenitic steel with a corrosion-resistant inner (clad) surface. Reactor pressure vessel (RPV) made of 2 ¼ Cr -1Mo-V bainitic steel having a good mechanical strength and creep resistance.
Ex vivo effect of JAK inhibition on JAK-STAT1 pathway hyperactivation in patients with dominant negative STAT3 mutations
Authors: Neth Olaf , Lobo Pilar Blanco , Hernández Paloma Guisado , Casaled Isabel Villaoslada , Carrillo Beatriz de Felipe , Carreras Carmen , Rodriguez Hector , Carazo-Gallego Begoña , Echevarria Ana Méndez- , Lucena José Manuel , Aljaro Pilar Ortiz , Castro María José , Noguera-Uclés José Francisco , Milner Joshua D. , McCann Katelyn , Zimmerman Ofer , Freeman Alejandra F. , Lionakis Michail S. , Holland Steven M. , Olbrich Peter 
Published: February 2022

Abstract
Purpose: STAT1 gain-of-function (GOF) and dominant negative (DN) STAT3 syndromes share clinical manifestations including infectious and inflammatory manifestations. Targeted treatment with Janus-kinase (JAK) inhibitors show promising results in treating STAT1 GOF-associated symptoms whilst management of DN STAT3 patients has been largely supportive. We here assessed the impact of ruxolitinib on the JAK-STAT1/3 pathway in DN STAT3 patients’ cells. Methods: Using flow cytometry, immunoblot, qPCR and ELISA techniques, we examined the levels of basal STAT1 and phosphorylated STAT1 (pSTAT1) of cells obtained from DN STAT3, STAT1 GOF patients and healthy donors following stimulation with type I/II interferons (IFNs) or interleukin (IL)-6. We also describe the impact of ruxolitinib on cytokine-induced STAT1 signaling in these patients.Results: DN STAT3 and STAT1 GOF resulted in a similar phenotype characterized by increased STAT1 and pSTAT1 levels in response to IFNα (CD3+ cells) and IFNg (CD14+ monocytes). STAT1-downstream gene expression and C-X-C motif chemokine 10 secretion were higher in most DN STAT3 patients upon stimulation compared to healthy controls. Ex vivo treatment with the JAK1/2-inhibitor ruxolitinib reduced cytokine responsiveness and normalized STAT1 phosphorylation in DN STAT3 and STAT1 GOF patient’ cells. In addition, ex vivo treatment was effective in modulating STAT1 downstream signaling in DN STAT3 patients.Conclusion: In the absence of effective targeted treatment options for AD-HIES at present, modulation of the JAK/STAT1 pathway with JAK inhibitors may be further explored particularly in those AD-HIES patients with autoimmune and/or autoinflammatory manifestations.
Efficacy of early goal-directed therapy using FloTrac/EV1000 to improve postoperative outcomes in patients undergoing off-pump coronary artery bypass surgery: a randomized controlled trial
Authors: Tribuddharat Sirirat , Sathitkarnmanee Thepakorn , Ngamsangsirisup Kriangsak , Sornpirom Sanpicha 
Published: February 2022

Abstract
Background: Early goal-directed therapy (EGDT) using FloTrac reduced length of stay (LOS) in intensive care (ICU) and hospital among patients undergoing coronary artery bypass graft (CABG) with a cardiopulmonary bypass (CPB). However, this platform in off-pump CABG (OPCAB) has received scant attention, so we evaluated the efficacy of EGDT using FloTrac/EV1000 as a modality for improving postoperative outcomes in patients undergoing OPCAB.Methods: Forty patients undergoing OPCAB were randomized to the EV1000 or Control group. The Control group received fluid, inotropic, or vasoactive drugs (at the discretion of the attending anesthesiologist) to maintain a mean arterial pressure 65-90 mmHg; central venous pressure 8-12 mmHg; urine output ≥ 0.5 mL·kg-1·h-1; SpO2 > 95%; and hematocrit ≥ 30%. The EV1000 group achieved identical targets using information from the FloTrac/EV1000. The goals included stroke volume variation (SVV) < 13%; stroke volume index (SVI) of 33-65 mL·beat-1·m-2 and cardiac index (CI) of 2.2-4.0 L·min-1·m-2; and systemic vascular resistance index (SVRI) of 1,500‑2,500 dynes s-1·cm-5·m-2.Results: The EV1000 group had a shorter LOS in ICU (mean difference -31.6 h, 95% CI -42.8 to -20.3; P < 0.001). The ventilator time for both groups was comparable (P = 0.316), but the hospital stay for the EV1000 group was shorter (mean difference -1.4 d, 95% CI -2.1 to -0.6; P < 0.001).Conclusions: EGDT using FloTrac/EV1000 compared to conventional protocol reduces LOS in ICU and hospital among patients undergoing OPCAB.Trial registration: This study was retrospectively registered at www.ClinicalTrials.gov (NCT04292951) on 3 March 2020.