bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel missense variant of LAMC1 as a possible cause of premature ovarian insufficiency in a Chinese family
Authors: Xu Huanfang , Wang Chunyan , Wei Han , Li Tengyan , Fang Yigong , Wang Binbin 
Published: February 2022

Abstract
Background: Premature ovarian insufficiency (POI) results in the loss of ovarian function and reproductive impairment. The cause of most cases of POI is unclear, although genetic factors are thought to be involved. This study aimed to identify novel pathogenic genes and variants in a Chinese family with POI.Results: An assessment of the family pedigree suggested that POI was inherited in an autosomal dominant manner in this family. Whole-exome sequencing of the proband and her affected mother identified a novel heterozygous missense variant in the laminin subunit gamma-1 gene (LAMC1; NM_002293.4:c.A3281T:p.D1094V). This variant was not found in any public databases, and was highly conserved among mammals. Online software predicted it to be deleterious with respect to protein function. Its presence in the POI family was confirmed by Sanger sequencing.Conclusions: We report a novel heterozygous missense variant in LAMC1 in a Chinese POI family, which was inherited in an autosomal dominant manner. This variant may result in the development of POI. Our results provide supporting evidence for a causative role for LAMC1 variants in POI.
Extraventricular Neurocytoma: a Case Report and Literature Review
Authors: Wang Liping , Dai Xiaoxiao , Ping Yifang , Chen Ping , Chen Yanming , Cheng Shan , Li Yongdong , Li Suwen , Li Haoran , Yuan Jiaqi , Hou Yanghao , Dong Jun 
Published: February 2022

Abstract
Extraventricular neurocytoma (EVN) is a rare intracranial tumor. The most common locations of EVN were frontal lobe and cerebellum, followed by temporal lobe. Heterogeneous contrast enhancement, cystic changes, perilesional edema and calcification were a few distinct imaging characteristics. EVN is a primary tumor with either glial or neuronal differentiation or potential for atypical changes. The lack of characteristic manifestations in histopathology makes EVN difficult to distinguish from other low-grade neuronal and mixed neuronal-glial tumors. Herein, we describe a case of frontal extraventricular neurocytoma in a 37-year-old male presenting with dizzy and review the literature on this rare tumor. Pathological examination reveals that the tumor mainly composed of oligodendroglia-like cells, with expression of synaptophysin (SYN) and Olig2 (oligodendrocyte transcription factor 2), and microcalcifications. Methylation profile and t-SNE analysis show our sample cluster with the other two reference EVNs. Next-generation sequencing (NGS) shows FGFR1-TACC1 fusion. We report a case of EVN with expression of Olig2, FGFR1-TACC1 fusion, methylation of MGMT promoter, and discuss our experience along with a review of the published literature.
Mechanism of novel enoxaparin sodium high viscosity bone cement reducing thrombosis by regulating the expression of CD40 in endothelial cells
Authors: Sang Linchao , Hao Kangning , Ding luobin , Shen Xiaoyu , Fu Dehao , Qi Xiangbei 
Published: February 2022

Abstract
Objective: To demonstrate, in a simple and reproducible animal model, that ordinary high viscosity bone cements develop local thrombi; To reduce the occurrence of thrombus in joint arthroplasty, we develop a new material, enoxaparin sodium high viscosity bone cement, to reduce local thrombosis and preliminarily explored the molecular mechanism by which it reduced local thrombus occurrence compared with ordinary high viscosity bone cement. Methods: We used New Zealand rabbits to establish two groups of animal models: ordinary high viscosity bone cement group and new enoxaparin sodium high viscosity bone cement group on the basis of carotid artery vein extracorporeal shunt. There were 3 rabbits in each group. The experimental samples were collected 4 hours after modeling, and the amount of thrombosis between the two groups was compared, The collected vascular tissue samples were quantitatively detected for endothelial cell related thrombomodulin.Results: we successfully established the animal model on the basis of arteriovenous shunt in New Zealand rabbits. We found that both ordinary high viscosity bone cement and new enoxaparin sodium high viscosity bone cement could form thrombosis. The weight of thrombosis in ordinary high viscosity bone cement group was 0.00706 ± 0.00136g/cm, The weight of thrombosis in the new enoxaparin sodium high viscosity bone cement group was 0.00551 ± 0.00115g/cm. The amount of thrombosis in the new enoxaparin sodium high viscosity bone cement group was significantly reduced. We detected by RT qPCR and Western blot that the expression of CD40, a related protein that can regulate thrombosis in vascular endothelial cells, was significantly lower in the new enoxaparin sodium high viscosity bone cement group than that in the normal high viscosity bone cement group.Conclusion: We confirm that the new enoxaparin sodium high viscosity bone cement forms less thrombus than common high viscosity bone cement and preliminarily elucidate that the molecular mechanism by which this new material reduces thrombus formation is to decrease the expression of thrombus associated regulatory protein CD40 in vascular endothelial cells.
Finite-time synchronization of different dimensional chaotic systems with uncertain parameters and external disturbances
Authors: Li Juan , Zheng Jiming 
Published: February 2022

Abstract
This paper proposes a new control scheme using two scaling matrices that realizes the finite-time synchronization of different-dimensional chaotic systems with parameter uncertainties and external disturbances. Firstly, based on Lyapunov stability theorem and finite-time stability theorem, the definition of finite-time synchronization of chaotic systems with different dimensions is introduced. Secondly, in the case of external disturbance and parameter uncertainty, an adaptive feedback hybrid controller and parameter adaptive laws are designed to synchronize different dimensional uncertain chaotic systems in finite-time. Then, according to the characteristics of the unknown parameters of the system, a transformation matrix is constructed to meet the needs of chaotic systems with different dimensions, and a simplified synchronization control scheme is designed. Finally, two numerical experiments are carried out to verify the effectiveness of the proposed methods.
Magnetocaloric Effect in ScGdHo Medium-entropy Alloy
Authors: Uporov Sergey , Sterkhov Evgenii , Balyakin Iliya 
Published: February 2022

Abstract
The search for eﬀective solid-state refrigerant for magnetocaloric application is one of the main-stream topics in materials science. Rareearth multi-principal element alloys seem to be promising candidates for these purposes. In this study, we address the issues of structure formation, magnetic and magnetocaloric properties in ScGdHo medium entropy alloy. We perform the ab initio molecular dynamics simulations for the liquid and solid phases in this system and reveal a strong tendency to form a single-phase solid solu-tion. The experimentally fabricated alloy demonstrates a single-phase HCP crystalline structure with high density of defects. The magnetic measurements reveal a complicated magnetic behaviour in the material. The alloy exhibits antiferromagnetic or ferromagnetic behavior depending on the magnetic ﬁeld. We have found that AFM order is preferable under a weak magnetic ﬁeld and FM order occurs when the ﬁeld strength exceeds 12 kOe. The alloy reveals a rather large coercive force in a wide temperature range up to the Neel point. The values of the magnetic entropy change and relative cooling power calculated for a magnetic ﬁeld change of 0-5 T are 8.93 Jkg−1K−1 and 938 J/kg, respectively. The examined ScGdHo alloy demonstrates the best magnetocaloric properties among similar rareearth metal materials discovered to date.
MEEMD Decomposition-Prediction-Reconstruction Model of Precipitation
Authors: Wang Yongtao , Liu Jian , Li Rong , Suo Xinyu , Lu EnHui 
Published: February 2022

Abstract
Aiming at the low prediction accuracy of precipitation time series data, proposes an Modified Ensemble Empirical Mode Decomposition-prediction-reconstruction model（MDPRM）. First,The non-stationary precipitation time series is decomposed into multiple decomposition terms by MEEMD. Then, according to the characteristics of different decomposition items,the particle swarm optimization support vector machine (PSO-SVM), convolutional neural network (CNN) and recurrent neural network (RNN) models are used for prediction respectively. Finally, the prediction results of each decomposition item are superimposed and reconstructed to form the final prediction results. The summer precipitation in the Wujiang River Basin in Guizhou Province from 1961 to 2018 was used as an example to verify the results. The results show that:compared with BP, SVM, RNN and other prediction methods, the MDPRM proposed in this paper has higher prediction accuracy, and can effectively guide the regional water resources allocation and scheduling, drought relief and disaster reduction.
Immortalized mesenchymal stem cells: a safe cell source for cellular or cell membrane-based treatment of glioma
Authors: Zhang Yuxuan , Liu Jie , Mo Yunzhao , Chen Zetao , Chen Taoliang , Li Yan , Zheng Yaofeng , Deng Shaokang , Xu Xiangdong , Chen Huajian , He Haoqi , Chen Jiansheng , Jin Tao , Sun Xinlin , Ke Yiquan , Wang Jihui 
Published: February 2022

Abstract
Mesenchymal stem cells (MSCs) have emerged as putative therapeutic tools due to their intrinsic tumour tropism, and anti-tumour and immunoregulatory properties. The limited passage and self-differentiation abilities of MSCs in vitro hinder preclinical studies on them. In this study, we focused on the safety of immortalized mesenchymal stem cells (im-MSCs) and, for the first time, studied the feasibility of im-MSCs as candidates for the treatment of glioma. The im-MSCs were constructed by lentiviral transfection of genes. The proliferative capacity of im-MSCs and the proliferative phenotype of MSCs and MSCs co-cultured with glioma cells (U87) were measured using CCK-8 or EdU assays. After long-term culture, karyotyping of im-MSCs was conducted. The tumorigenicity of engineered MSCs was evaluated using soft agar cloning assays. Next, the engineered cells were injected into the brain of female BALB/c nude mice. Finally, the cell membranes of im-MSCs were labelled with DiO or DiR to detect their ability to be taken up by glioma cells and target in situ gliomas using the IVIS system. Engineered cells retained the Immunophenotype of MSC; im-MSCs maintained the ability to differentiate into mesenchymal lineages in vitro; and im-MSCs showed stronger proliferative capacity than unengineered MSCs but without colony formation in soft agar, no tumorigenicity in the brain, and normal chromosomes. MSCs or im-MSCs co-cultured with U87 cells showed enhanced proliferation ability, but did not show malignant characteristics in vitro. Immortalized cells continued to express homing molecules. The cell membranes of im-MSCs were taken up by glioma cells and targeted in situ gliomas in vivo, suggesting that im-MSCs and their plasma membranes can be used as natural drug carriers for targeting gliomas, and providing a safe, adequate, quality-controlled, and continuous source for the treatment of gliomas based on whole-cell or cell membranes carriers.
Living with a Frozen Shoulder – A Phenomenological Inquiry.
Authors: Lyne Suellen Anne , Goldblatt Fiona Mary , Shanahan Ernst Michael 
Published: February 2022

Abstract
Background : Frozen shoulder (adhesive capsulitis) is an inflammatory condition affecting the capsule of the glenohumeral joint. It is characterised by a painful restricted range of passive and active movement in all planes of motion. The impact of frozen shoulder on affected individuals remains poorly characterised. In this study we sought to better understand the lived experience of people suffering from frozen shoulder to characterise the physical, psychological and socioeconomic impact of the condition. Methods: A qualitative study using a phenomenological approach was undertaken. Purposeful sampling was used to identify individuals for interview. Semi-structured interviews were performed and continued until saturation was achieved. A biopsychosocial framework was used during the analysis in order to generate themes which best described the phenomenon and reflected the lived experience of individuals’ suffering from this condition. Results: Five main themes emerged including; the severity of the pain experience, a loss of independence, an altered sense of self, the significant psychological impact, and the variable experience with healthcare providers. Conclusions: These findings offer an insight into the lived experience of individuals with frozen shoulder, both on a personal and sociocultural level. The pain endured has profound impacts on physical and mental health, with loss of function resulting in a narrative reconstruction and altered sense of self. Our findings illustrate that frozen shoulder is much more than a benign self-limiting musculoskeletal condition and should be managed accordingly.Trial Registration: ANZCTR 12620000677909 Registered 28/04/2020 https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=379719&isReview=true
Identification of pterygium-related conjunctival epithelium microRNA and mRNA expression profiles by high-throughput screening analysis
Authors: Jianfeng Yu , Pengfei Li , Chen Xiaojuan , Zhang Guowei , Huaijin Guan 
Published: February 2022

Abstract
Purpose. To detect the hub mRNAs and construct a microRNA (miRNA)-mRNA regulatory network by comparing pterygium-related conjunctival epithelium (PCE) and normal conjunctival epithelium (NCE) patient samples by high-throughput sequencing.Methods. Bulbar conjunctival epithelium samples were obtained from patients with primary pterygium and from donated normal eyeballs. Bioinformatics analysis, which included differential gene expression, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis, was applied for the functional enrichment analysis of differentially expressed mRNAs (DEmRNAs). Protein-protein interaction (PPI) analysis and gene set enrichment analysis (GSEA) were performed on DEmRNAs whose pathways were related to the development of pterygium. Potential miRNAs binding to these hub DEmRNAs were predicted using software, and a hub mRNA-miRNA regulatory network was constructed. MiRNAs were verified through RT-qPCR.Results. Overall, the results showed 280 upregulated and 256 downregulated DEmRNAs. To explore the potential functions of DEmRNAs, we performed bioinformatics analysis to detect the potential functions of DEmRNAs and the relevant pathways related to the pathogenesis of pterygium in PCE. In addition, we enriched six pathways that were involved in the development of pterygium using a heatmap. Then, the GSEA results showed that galactose metabolism, epithelial mesenchymal transition and so on were significantly enriched among upregulated mRNAs (P＜0.001, and NES ≥ 1). Cytokine-cytokine receptor interaction, primary immunodeficiency, MAPK signalling pathway and so on were significantly enriched among downregulated mRNAs (P＜0.001, and NES ≤ -1). Then, a miRNA/mRNA regulatory network was constructed with 20 miRNAs and 48 differentially expressed genes (DEGs). The RT-qPCR validation study confirmed nine downregulated miRNAs (miR-653-3p, miR-3617-5p, miR-4681, miR-4802-3p, miR-888-3p, miR-7106-3p, miR-543, miR-200b-3p and miR-203a-3p) and two upregulated miRNAs (miR-5579b-3p and miR-6844).Conclusion. Our study shows the mRNA expression profile and miRNA/mRNA regulatory network of the PCE and provides a new perspective on the pathogenesis of pterygium.
Neuroprotective Effects of Propylgallate Against Oxidative Stress in Retinal Ganglion Cells
Authors: Xiao Qin , Lu Zhanjun , Lu Jinsong , Tao Chun , Ma Ruitong , Wang Eerdun 
Published: February 2022

Abstract
Background: Diabetic retinopathy is a group of eye diseases which result in damage to the optic nerve and vision loss, it has seriously affect peoples' health. The purpose of this study is to contrast the neuroprotective effects of curcumin, gastrodin, propylgallate, adenosine. At the same time, we preliminarily explore the molecular mechanism of protective drugs.Methods: In this study, we used 500μM H2O2 treated RGC-5 cells to induce a cellular oxidative stress model. We treated this cell model with four drug monomers: Propylgallate, Curcumin, Gastrodin and Adenosine to find drug monomers with neuroprotective effect. We used apoptosis PCR array to obtain apoptosis related genes regulated by neuroprotective drugs.Results: We found the Propylgallate treated RGC-5 cells had highest survival rate when compared to Curcumin, Gastrodin, Adenosine treated RGC-5 cells.In addition, it had lowest cell cytotoxicity and apoptotic rate when compared to Curcumin, Gastrodin, Adenosine treated RGC-5 cells.Moreover, the expression of ROS in Propylgallate treated RGC-5 cells was lowest when compared to Curcumin, Gastrodin, Adenosine treated RGC-5 cells. We found that Caspase-3, Caspase-8, and Caspase-9 are the main target genes of Propylgallate which can preliminarily explain the neuroprotective mechanism of Propylgallate against apoptosis..Conclusion: The present study revealed that the propylgallate has best neuroprotective effects, it may provide a promissing drug to prevent and improve the damage of optic nerve. In this article, we also preliminarily expounded the neuroprotective molecular mechanism of Propylgallate.
An Adaptive On-demand Routing Protocol With QoS Support for urban-MANETs
Authors: Khanh Quy Vu 
Published: February 2022

Abstract
Mobile Mobile Ad Hoc Networks (MANETs) are organizations of mobile network devices that ability of self-configuring and self-setting parameters for effective in-network communication. Thanks to the flexibility and convenience in establishing connections and transfer data, MANETs have demonstrated outstanding capabilities and abilities in a wide range of fields serving humanity, such as healthcare, intelligent transportation systems, smart agriculture, smart retail, and IoT ecosystems. Due to the mobile characteristics of network nodes, the structure of MANETs changes frequently. Moreover, the operating principle of MANETs is distributed, not relying on central devices such as base stations, leading to the guarantee QoS problem is one of the main challenges of MANETs. In this study, we proposed a QoS-aware on-demand routing protocol (QoS-ADRP) for urban-MANET applications. To enhance the feasibility of the proposed solution, we establish a mechanism so that the proposed protocol can work on both Adaptive and Admission modes. The experiment results demonstrated that the QoS-ADRP protocol improved in terms of the QoS flows, packet delivery ratio, latency, throughput compared to existing protocols.
The gut bacterial microbiome of Nile tilapia (Oreochromis niloticus) from lakes across an altitudinal gradient
Authors: Bereded Negash Kabtimer , Abebe Getachew Beneberu , Fanta Solomon Workneh , Curto Manuel , Waidbacher Herwig , Meimberg Harald , Domig Konrad J 
Published: February 2022

Abstract
BackgroundMicroorganisms inhabiting the gut play a significant role in supporting fundamental physiological processes of the host, which contributes to their survival in varied environments. Several studies have shown that altitude affects the composition and diversity of intestinal microbial communities in terrestrial animals. However, little is known about the impact of altitude on the gut microbiota of aquatic animals. The current study examined the variations in the gut microbiota of Nile tilapia (Oreochromis niloticus) from four lakes along an altitudinal gradient in Ethiopia by using 16S rDNA Illumina MiSeq high-throughput sequencing. ResultsThe results indicated that low-altitude samples typically displayed greater alpha diversity. The results of principal coordinate analysis (PCoA) showed significant differences across samples from different lakes. Firmicutes was the most abundant phylum in the Lake Awassa and Lake Chamo samples whereas Fusobacteriota was the dominant phylum in samples from Lake Hashengie and Lake Tana. The ratio of Firmicutes to Bacteroidota in the high-altitude sample (Lake Hashengie, altitude 2440 m) was much higher than the ratio of Firmicutes to Bacteroidota in the low altitude population (Lake Chamo, altitude 1235 m). We found that the relative abundances of Actinobacteriota, Chloroflexi, Cyanobacteria, and Firmicutes were negatively correlated with altitude, while Fusobacteriota showed a positive association with altitude. Despite variability in the abundance of the gut microbiota across the lakes, some shared microbial communities were detected. ConclusionsIn summary, this study showed the indirect influence of altitude on gut microbiota. Altitude has the potential to modulate the gut microbiota composition and diversity of Nile tilapia. Future work will be needed to elucidate the functional significance of gut microbiota variations based on the geographical environment. Significance and Impact of the Study: Our study determined the composition and diversity of the gut microbiota in Nile tilapia collected from lakes across an altitude gradient. Our findings greatly extend the baseline knowledge of fish gut microbiota in Ethiopian lakes that plays an important role in this species sustainable aquaculture activities and conservation.
Impact of COVID-19 Pandemic on the Epidemiology of STDs in China: Based on the GM (1,1) Model
Authors: Yan Jingmin , Li Yanbo , Zhou Pingyu 
Published: February 2022

Abstract
Background: It has been reported that COVID-19 pandemic has greatly impacted on the epidemiological trend of Sexually Transmitted Diseases (STDs) in many countries. However, relevant researches are limited in China. We aimed to analyze the extent of COVID-19 pandemic impact on STDs in China.Methods: The incidence of HIV infection, syphilis and gonorrhea in China from 2008 to 2020 was collected. But only data of these three STDs from 2013 to 2018 were used to establish the Grey Model (1,1) considering about the impact of policies of China. Then we calculated the predictive incidence of each STD in 2019, 2020 and 2021 by the established Model. And then we estimated the extent of the impact of COVID-19 on the epidemiological changes of STDs by analyzing the difference between the absolute percentage error (APE) of the predictive incidence and actual rate in 2019 and 2020. Results: The incidence of HIV infection and syphilis showed an increasing trend from 2008 to 2019 in China, but that for gonorrhea was fluctuant. Of note, the incidence of these three STDs decreased significantly in 2020 compared with that in 2019. The APE of HIV infection, syphilis and gonorrhea in 2020 (20.54%, 15.45% and 60.88%) were about 7 times, 4 times and 2 times of that in 2019 (2.94%, 4.07% and 30.41%). The incidence of HIV infection, syphilis and gonorrhea would be 5.77/100,000, 39.64/100,000 and 13.19/100,000 in 2021 based on our model.Conclusions: Our analysis revealed that the epidemiological trend of STDs in China was significant influenced by COVID-19 pandemic. The result highlights the critical need for transformative strategies in STDs control in the context of the long-term effects of COVID-19 pandemic in the future.