bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dissociative disorder in Mayer Rokitansky Küster Hauser syndrome with pulmonary agenesis: a case report
Authors: Joshi Sulochana , Bhandari Anup Raj , Shrestha Prashant , Shakya Rabi 
Published: January 2022

Abstract
Background
The Mayer Rokitansky Küster Hauser (MRKH) syndrome is a rare congenital disorder characterized by the absence of uterus and vagina in a patient who is phenotypically a female, with 46 XX karyotypes. It affects 1 in 4000 to 5000 female new-borns. Pulmonary agenesis is a rare association in this MRKH syndrome. Females with MRKH face various mental health issues and psychological disturbances, including dissociative disorder which is a stress-related psychiatric disorder. Dissociative disorder in MRKH syndrome is under-recognized and under-treated.

Case presentation
A 23-year-old unmarried woman presented to the emergency forabnormal behaviour attack. Recurrent episodes of dissociative convulsions were present most of the time with MRKH syndrome as the pertinent stressor. Both antidepressants and psychotherapy helped to decrease the frequency of dissociative convulsions and come interms with the syndrome.

Conclusion
This case describes dissociative disorder as the presentation and comorbid condition of MRKH syndrome and the impact of MRKH syndrome on the patient. We attempt to explain the occurrence of dissociative disorder in MRKH syndrome and the results of under-recognition and under-treatment of the same. We aim to highlight the presence of commonly treatable conditions associated with a rare syndrome and its effect when untreated and unrecognized.
Arabidopsis NLP7 improves nitrogen use efficiency and yield in cotton
Authors: JAN Sami Ullah , LIAQAT Ayesha , ZHU Yonghong , LI Jing , ZHANG Huangyang , ABDALLA Mohnad , WU Jie , XIANG Chengbin , WU Shenjie , ALFATIH Alamin 
Published: January 2022

Abstract
Background
Nitrogen (N) is a required macronutrient for cotton growth and productivity. Excessive N fertilizers are applied in agriculture for crop yield maximization, which also generates environmental pollution. Improving crop N use efficiency (NUE) is the most economical and desirable way of reducing fertilizer application and environmental pollution. NUE has been an important issue in cotton. So far there is no report on cotton NUE improvement via transgenic approach. Nin-like proteins (NLP) are transcription factors regulating NUE. We previously demonstrated that AtNLP7 improved NUE and biomass when overexpressed in Arabidopsis. However, it is not known whether AtNLP7 can be used to improve NUE in crops.

Results
To test the feasibility, we expressed AtNLP7 in cotton and evaluated NUE and yield of the transgenic cotton in the field. Transgenic cotton showed improved NUE and yield under both low and high N conditions. In addition, plant biomass, amount of absorbed N, N contents, activities of N-assimilating enzymes, and the expression of N-related marker genes were significantly increased in transgenic cotton compared with the wild type control, suggesting that AtNLP7 enhances NUE in cotton.

Conclusion
Together, our results demonstrate that AtNLP7 is a promising candidate to improve NUE and yield in cotton.
Weight-loss in obese dogs promotes important shifts in fecal microbiota profile to the extent of resembling microbiota of lean dogs
Authors: Macedo Henrique Tobaro , Rentas Mariana Fragoso , Vendramini Thiago Henrique Annibale , Macegoza Matheus Vinicius , Amaral Andressa Rodrigues , Jeremias Juliana Toloi , de Carvalho Balieiro Júlio César , Pfrimer Karina , Ferriolli Eduardo , Pontieri Cristiana Ferreira Fonseca , Brunetto Marcio Antonio 
Published: January 2022

AbstractBackgroundAmong the undesirable changes associated with obesity, one possibility recently raised is dysbiosis of the intestinal microbiota. Studies have shown changes in microbiota in obese rats and humans, but there are still few studies that characterize and compare the fecal microbiota of lean, obese and dogs after weight loss. Thus, this study aimed to evaluate the effects of a weight loss program (WLP) in fecal microbiota of dogs in addition to comparing them with those of lean dogs. Twenty female dogs of different breeds, aged between 1 and 9 years were selected. They were equally divided into two groups: Obese group (OG), with body condition score (BCS) 8 or 9/9, and body fat percentage greater than 30%, determined by the deuterium isotope dilution method, and lean group (LG) with BCS 5/9, and maximum body fat of 15%. Weight loss group (WLG) was composed by OG after loss of 20% of their current body weight. Fecal samples were collected from the three experimental groups. Total DNA was extracted from the feces and these were sequenced by the Illumina methodology. The observed abundances were evaluated using a generalized linear model, considering binomial distribution and using the logit link function in SAS (p < 0.05).ResultsThe WLP modulated the microorganisms of the gastrointestinal tract, so that, WLG and LG had microbial composition with greater biodiversity than OG, and intestinal uniformity of the microbiota (Pielou’s evenness index) was higher in OG than WLG dogs (P = 0.0493) and LG (P = 0.0101). In addition, WLG had values of relative frequency more similar to LG than to OG.ConclusionThe fecal microbiota of the studied groups differs from each other. The weight loss program can help to reverse the changes observed in obese dogs.
The F-18 FDG PET/CT evaluation of the metastatic adrenal lesions of the non-lung cancer tumors compared with pathology results
Authors: Koç Zehra Pınar , Özcan Pınar Pelin , Sezer Emel , Eser Kadir , Kara Tuba 
Published: January 2022

Abstract
Background
The aim of this study was to evaluate the role of F-18 FDG PET/CT imaging in the diagnosis of primary and metastatic adrenal tumors that originate from non-lung cancer primary tumors.

Results
F-18 FDG PET/CT images of patients (8 male and 6 female; mean: 55.36 ± 16.2 years old) who attended with the diagnosis of primary or adrenal metastatic lesions other than lung cancer metastasis were evaluated in a retrospective manner. The diameter of the adrenal lesions was mean: 23.93 ± 36.6 mm with SUVmax levels of mean: 9.98 ± 7.8. The primary site of 2/3 of the patients with unknown primary was the adrenal gland, and in one of the patients primary tumor remained unknown during follow-up.

Conclusions
According to the results of this study, F-18 FDG PET/CT has high diagnostic performance in the diagnosis of the adrenal gland primary and metastatic lesions, which originate from non-lung cancer tumors. Further studies in the larger series are warranted.
The diagnostic value of digital breast tomosynthesis with complementary ultrasound in comparison with magnetic resonance imaging in assessment of postoperative changes and locoregional recurrence of breast cancer
Authors: Barakat Mai M. K. , Mohamed Eman K. A. E. , Habib Lobna A. E. , Ahmed Mortada Elsayed 
Published: January 2022

Abstract
Background
Digital breast tomosynthesis with complementary ultrasound is a powerful imaging modality in detection of breast cancer. Magnetic resonance imaging has many limitations due to its low specificity multiple pitfalls especially signal-to-noise ratio, as well as the spatial resolution. Our purpose of the study is to evaluate the role of digital breast tomosynthesis with complementary ultrasound compared to magnetic resonance imaging in the assessment of postoperative changes and locoregional recurrence of breast cancer.

Results
Our prospective study included thirty women who underwent conservative breast surgery. Digital breast tomosynthesis with complementary ultrasound revealed greater specificity (95.2%) than Magnetic resonance imaging (90.5%) in the detection of locoregional recurrence of breast cancer. On the other hand, it revealed the same sensitivity (88.9%). Subsequently, digital breast tomosynthesis with complementary ultrasound showed higher accuracy (93.3%) and higher positive predictive value (88.9%) than magnetic resonance imaging (90%), (80%), respectively. However, both of them revealed a comparable negative predictive value (95%).

Conclusion
Digital breast tomosynthesis with complementary ultrasound is a powerful imaging modality that can be used to detect any recurrence in patients who are surgically treated for breast cancer with higher specificity, accuracy than magnetic resonance imaging. Additionally, the use of these modalities enhances the diagnosis of surgically treated breast cancer for early detection of recurrence.
Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species
Authors: Magdy Mariam M. , Gaber Yasser , Sebak Mohamed , Azmy Ahmed F. , AbdelGhani Sameh 
Published: January 2022

Abstract
Background
Polycyclic aromatic hydrocarbons (PAHs) such as anthracene are one of the most toxic contaminants to our environment. Microbial biodegradation of these xenobiotics is a cost-effective technological solution. The present study aimed to recover some bacterial isolates from Beni-Suef Governorate in Egypt with high capabilities of anthracene biodegradation. The selected isolates were molecularly characterized by 16S rRNA gene sequencing, the degree of anthracene biodegradation was monitored using optical density (OD) and high-performance liquid chromatography (HPLC), PCR amplification of some selected genes encoding biodegradation of PAHs was monitored, and gas chromatography–mass spectrometry (GC–MS) analysis was applied for detecting the resulted metabolites.

Result
Three bacterial isolates were studied, the 16s rRNA sequences of the isolates showed homology of the first isolate to Brevibacillus sp. (94.58 %), the second isolates showed homology to Pseudomonas sp. (94.53%) and the third isolate showed homology to Methylocystis sp. (99.61 %), all isolates showed the ability to degrade anthracene. PCR amplification of some selected genes encoding biodegradation of PAHs revealed the presence of many biodegrading genes in the selected strains. Gas chromatography-mass spectrometry (GC–MS) analysis of the metabolites resulted from anthracene biodegradation in the present study suggested that more than one biodegradation pathway was followed by the selected isolates.

Conclusions
The selected strains could represent a potential bioremediation tool in solving the PAHs problem in the Egyptian environment with a clean and cost-effective technique.

Graphical Abstract
Pneumomediastinum: retrospective analysis of 19 cases and an innovation proposal in classification
Authors: Çetin Mehmet , Türk İlteriş , Fındık Göktürk , Aydoğdu Koray , Gülhan Selim Şakir Erkmen , Bıçakçıoğlu Pınar , Kaya Sadi 
Published: January 2022

Abstract
Background
Guidelines to standardize treatment and follow-up strategies in pneumomediastinum cases are lacking. The aim of the study was to evaluate the etiology in pneumomediastinum cases and the results of treatment and follow-up.

Results
Nineteen patients with pneumomediastinum who were followed up in our clinic between 2015 and 2020 comprised the study population. Among the patients, 16 (84.2%) were male, and the mean age was 31.15 years. The chief presenting complaints were chest pain and dyspnea. Pneumomediastinum was spontaneous in 15/19 patients (including spontaneous pneumomediastinum with an underlying pathology in 3/15), traumatic in 3/19, and iatrogenic in 1/19. Spontaneous pneumomediastinum without underlying pathology was seen in younger adults (mean age: 23 years). Surgical intervention in traumatic and iatrogenic pneumomediastinum cases was compared with spontaneous cases and no statistically significant difference was observed (p=0.178). The mean hospital stay of all patients was 3.15 days. Only one patient had a recurrence and died, which was later determined to be a secondary spontaneous pneumomediastinum case.

Conclusion
Pneumomediastinum often occurs with an underlying pathology in advancing age and as spontaneous in younger patients. Therefore, “secondary spontaneous pneumomediastinum” subclass should be evaluated in the classification to facilitate to create a standard guideline and prevent overdiagnosis and overtreatment.
Development of impact attenuator analysis tools in crash scenario using Euler method and finite element analysis
Authors: Athafarras Malik , Djamari Djati Wibowo , Fikri Muhamad Rausyan , Budiman Bentang Arief , Triawan Farid , Veza Ibham 
Published: January 2022

AbstractThe problem considered in this work is the development of simulation method for simulating car crash which utilizes simple car—impact attenuator model developed in MATLAB. Usually, car crash simulation is done using full finite element simulation which could take hours or days depending on the model size. The purpose of proposed method is to achieve quick results on the car crash simulation. Past works which utilizes simple car—impact attenuator model to simulate car crash use continuous time model and the impact attenuator parameter is obtained from the experimental results. Different from the related works, this work uses discrete time model, and the impact attenuator parameter is obtained from finite element simulation. Therefore, the proposed simulation method is not only achieving quick simulation results but also minimizing the cost and time in obtaining the impact attenuator parameter. The proposed method is suitable for parametric study of impact attenuator.
Co-creation methodology with Smart technologies in Health and well-being to enable communication between isolated and disperse small communities: a literature review
Authors: RAMOS Victoria ,   MELTIC partners, de San Pedro Iris , Casado Elvira , Santacruz Esmeralda , Hernández Coral , Alcoceda Jose A. , de Tena-Davila M.Jose , Burgos Alfredo , Perez de la Camara Santiago , Marina-Boillos Pablo , Pascual Mario 
Published: January 2022

Objective: The objective is to determine reported cases of co-creation methodology about the use of smart technologies in public spaces in order to create new forms of social interactions and practices, which in turn creates new socio-spatial relations and promotes interactions and communication between isolated and disperse communities. Methods: The literature published in the last 5 years (2016-2020) has been reviewed. Searches on Co-creation methodology and ICTs in Health and Biomedicine, on topics such as interaction among users, ICT and social behaviour, spatial analyses, planning methodologies and public involvement, on-line gaming, self‐learning, and the prevention of risky habbits are made manually. Results: Search strategies developed through electronic databases and manual search identified a total of 180 references, included in the supplementary material. They have been divided by the technologies used in the studies, co-creation methodology, and according to the type of socio-medical application. This research highlights the penetration of ICT in social and healthcare environments and clearly demonstrates the high number of publications that have come out over recent years and a lack of publications that evaluate co-creation methodology in this field. Conclusions: Most of the papers included only partially cover the subject matter of ICT in Health and Biomedicine and how to use smart technologies to transform public spaces in small communities into people-friendly human environments. The research carried out for this paper clearly demonstrates the high number of publications concerning technology assessment. However, there is a distinct lack of publications that evaluate co-creation methodology.
Passively mode-locked Er-doped fiber laser based on a ferromagnetic insulator Cr2Si2Te6 as a saturable absorber
Authors: Yang Fuhao , Sun Shuo , Chen Si , Wang Yanjuan , Sui Zhiqi , Zhu Mingxiao , Hong Zhifeng , Xu Jianghao , Tang Shouqian , Lu Cheng , Wang Guomei , Fu Shenggui , Chen Xiaohan , Zhang Huanian , Zhang Wenfei 
Published: January 2022

In our work, a new-type, to the best of our knowledge, ferromagnetic insulator and its nonlinear optical absorption characteristics and related ultrafast modulation applications were investigated.

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with excellent saturable absorption characteristics can be used as a SA to obtain ultrafast pulse lasers.
Phlebotomine sand flies (Diptera: Psychodidae) of the Maghreb region: A systematic review of distribution, morphology, and role in the transmission of the pathogens
Authors: Benallal Kamal Eddine , Garni Rafik , Harrat Zoubir , Volf Petr , Dvorak Vít 
Published: January 2022

BackgroundPhlebotomine sand flies (Diptera: Psychodidae) are important vectors of various human and animal pathogens such asBartonella bacilliformis,Phlebovirus, and parasitic protozoa of the genusLeishmania, causative agent of leishmaniases that account among most significant vector-borne diseases. The Maghreb countries Mauritania, Morocco, Algeria, Tunisia, and Libya occupy a vast area of North Africa and belong to most affected regions by these diseases. Locally varying climatic and ecological conditions support diverse sand fly fauna that includes many proven or suspected vectors. The aim of this review is to summarize often fragmented information and to provide an updated list of sand fly species of the Maghreb region with illustration of species-specific morphological features and maps of their reported distribution.Materials and methodsThe literature search focused on scholar databases to review information on the sand fly species distribution and their role in the disease transmissions in Mauritania, Morocco, Algeria, Tunisia, and Libya, surveying sources from the period between 1900 and 2020. Reported distribution of each species was collated using Google Earth, and distribution maps were drawn using ArcGIS software. Morphological illustrations were compiled from various published sources.Results and conclusionsIn total, 32 species of the generaPhlebotomus(Ph.) andSergentomyia(Se.) were reported in the Maghreb region (15 from Libya, 18 from Tunisia, 23 from Morocco, 24 from Algeria, and 9 from Mauritania).Phlebotomus mariaeandSe. africanasubsp.asiaticawere recorded only in Morocco,Ph. mascitti,Se. hirtus, andSe. tiberiadisonly in Algeria, whereasPh. duboscqi,Se. dubia,Se. africana africana,Se. lesleyae,Se. magna, andSe. freetownensiswere reported only from Mauritania. Our review has updated and summarized the geographic distribution of 26 species reported so far in Morocco, Algeria, Tunisia, and Libya, excluding Mauritania from a detailed analysis due to the unavailability of accurate distribution data. In addition, morphological differences important for species identification are summarized with particular attention to closely related species such asPh. papatasiandPh. bergeroti,Ph. chabaudi, andPh. riouxi, andSe. christophersiandSe. clydei.
A standardised Phase III clinical trial framework to assess therapeutic interventions for Lassa fever
Authors: Olayinka Adebola Tolulope , Bourner Josephine , Akpede George O. , Okoeguale Joseph , Abejegah Chukwuyem , Ajayi Nnennaya A. , Akude Christian , Ayodeji Oluwafemi , Bausch Daniel G. , de Clerck Hilde , Dan-Nwafor Chioma , Dunning Jake , Erameh Cyril , Eze Justus Ndulue , Formenty Pierre , Gillesen Annelies , Jalloh Sulaiman , Jaspard Marie , Jegede Tolulope , Maikere Jacob , Malvy Denis , Ogbaini-Emovon Ephraim , Ojo Olalekan Ezekial , Okogbenin Sylvanus , O’Neill Kwame , Orji Maria-Lauretta , Owhin Sampson Omagbemi , Ramharter Michael , Samuels Robert J. , Shehu Nathan , Merson Laura , Salam Alex Paddy , Kayem Nzelle Delphine , Horby Peter , Ihekweazu Chikwe , Olliaro Piero 
Published: January 2022

BackgroundOnly one recommendation currently exists for the treatment of Lassa fever (LF), which is ribavirin administered in conjunction with supportive care. This recommendation is primarily based on evidence generated from a single clinical trial that was conducted more than 30 years ago–the methodology and results of which have recently come under scrutiny. The requirement for novel therapeutics and reassessment of ribavirin is therefore urgent. However, a significant amount of work now needs to be undertaken to ensure that future trials for LF can be conducted consistently and reliably to facilitate the efficient generation of evidence.MethodologyWe convened a consultation group to establish the position of clinicians and researchers on the core components of future trials. A Core Eligibility Criteria (CEC), Core Case Definition (CCD), Core Outcome Set (COS) and Core Data Variables (CDV) were developed through the process of a multi-stakeholder consultation that took place using a modified-Delphi methodology.ResultsA consensus position was achieved for each aspect of the framework, which accounts for the inclusion of pregnant women and children in future LF clinical trials. The framework consists of 8 core criteria, as well as additional considerations for trial protocols.ConclusionsThis project represents the first step towards delineating the clinical development pathway for new Lassa fever therapeutics, following a period of 40 years without advancement. Future planned projects will bolster the work initiated here to continue the advancement of LF clinical research through a regionally-centred, collaborative methodology, with the aim of delineating a clear pathway through which LF clinical trials can progress efficiently and ensure sustainable investments are made in research capacity at a regional level.
Serum miR-34a-5p and miR-199a-3p as new biomarkers of neonatal sepsis
Authors: Abdelaleem Omayma O. , Mohammed Shereen Rashad , El Sayed Hassan S. , Hussein Sherin Khamis , Ali Doaa Y. , Abdelwahed Mostafa Y. , Gaber Sylvana N. , Hemeda Nada F. , El-Hmid Rehab G. Abd 
Published: January 2022

Background
Neonatal sepsis is a serious condition. Recent clinical studies have indicated that microRNAs (miRNAs) are key players in the pathogenesis of sepsis, which could be used as biomarkers for this condition.

Patients and methods
A total of 90 neonates with sepsis and 90 healthy neonates were enrolled in this study. qRT-PCR was performed to measure the expression levels of serum miR-34a-5p and miR-199a-3p.

Results
miR-34a-5p and miR-199a-3p serum levels were significantly reduced in neonates with sepsis compared with those in healthy neonates (P = 0.006 and P = 0.001, respectively). Significant correlations of miR-34a-5p and miR-199a-3p with each of TLC, RDW, RBS, and C-reactive protein (CRP) as well as SNAPII were observed, indicating their associations with the severity of neonatal sepsis.

Conclusion
miR-34a-5p and miR-199a-3p may be useful as novel biomarkers in neonatal sepsis and may provide a new direction for its treatment.
Spontaneous Infection of a Nonmalignant Intraductal Papillary Mucinous Neoplasm
Authors: Ashhab Ashraf A. , Podboy Alexander , Lo Simon K. 
Published: January 2022

ABSTRACT
Intraductal papillary mucinous neoplasm (IPMN) is a pancreatic tumor that originates from the epithelium of the pancreatic duct. Although IPMN cysts can be complicated by infection, this has been reported to involve cysts that have ruptured, fistulized into surrounding organs, undergone malignant transformation, or were recently sampled. We present a 76-year-old man with a history of an IPMN who developed spontaneous cyst infection which was managed with fine-needle aspiration and antibiotics. To the best of our knowledge, this is the first reported case of spontaneous infection of a nonmalignant IPMN. Cyst infection should be considered as a very rare cause of unexplained fevers in patients with history of IPMN.