bluuebunny/crossref_metadata_2025_split huggingface dataset:
CNBP restricts SARS-CoV2 by regulating IFN and disrupting RNA-protein condensates
Authors: Fitzgerald Katherine , Chen Yongzhi , Lei Xuqiu , Jiang Zhaozhao , Humphries Fiachra , Mustone Nicholas , Ramos Irene , Mutetwa Tinaye , Fernandez-Sesma Ana 
Published: May 2022

Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) evades antiviral immunity through the expression of viral proteins that block detection, signaling, interferon (IFN) induction, and IFN-stimulated gene (ISG) expression. Weak induction of type I IFNs is associated with a hyperinflammatory response in patients that develop severe COVID-19. Here we uncover a role for cellular nucleic acid-binding protein (CNBP) in restricting SARS-CoV-2. Typically, CNBP resides in the cytosol and, in response to RNA sensing pathways, undergoes phosphorylation, nuclear translocation, and IFNβ enhancer DNA binding to turn on IFNβ gene transcription. In SARS-CoV-2-infected cells CNBP coordinates IFNβ gene transcription. In addition, CNBP binds SARS-CoV-2 viral RNA directly. CNBP competes with the nucleocapsid (N) protein and prevents viral RNA and nucleocapsid protein from undergoing liquid-liquid phase separation (LLPS) forming condensates critical for viral replication. Consequently, cells and animals lacking CNBP have higher viral loads and CNBP-deficient mice succumb rapidly to infection. Altogether, these findings identify CNBP as a key antiviral factor for SARS-CoV-2, functioning both as a regulator of antiviral IFN gene expression and a cell intrinsic restriction factor that disrupts LLPS to limit viral replication and spread.
Low skeletal muscle mass may contribute to the progression of non-alcoholic fatty liver disease in men
Authors: Pan Xiao-Yan , Liu Wen-Yue , Zhu Pei-Wu , Li Gang , Tang Liang- Jie , Gao Feng , Huang Ou-Yang , Yuan Hai-Yang , Targher Giovanni , Byrne Christopher D. , Wang Xiao-Dong , Zheng Ming-Hua 
Published: May 2022

Abstract
Background/Purpose of the study: Although low skeletal muscle mass is associated with non-alcoholic fatty liver disease (NAFLD), it is currently uncertain whether there are associations between weight-adjusted appendicular skeletal muscle (ASM%), severity of histological features of NAFLD, and the patatin-like phospholipase domain-containing 3 (PNPLA3) rs738409 polymorphism. Our aim was to test for a modifying influence of the PNPLA3 rs738409 variant on the associations between ASM% and severity of NAFLD histological features.Methods: We enrolled 401 Chinese men with biopsy-proven NAFLD. Using a bioelectrical-impedance body composition analyzer (BIA, Inbody 720, Japan Inc., Tokyo), we calculated the ASM% as the percentage of total appendicular skeletal muscle mass (ASM, kg)/total body mass (kg) ×100.Results: Compared to those with high ASM%, patients with low ASM% (≤30.6) had a greater severity of individual histological features of NAFLD. These patients also had a higher risk of severe steatosis and non-alcoholic steatohepatitis (NASH) (adjusted-odds ratio [OR] 2.34, 95%CI 1.39-3.93, and adjusted-OR 2.22, 95%CI 1.30-3.77) even after adjustment for age, body mass index, diabetes, and serum creatinine levels. Carriage of the G allele of PNPLA3 rs738409 plus low ASM% was associated with an increased risk of severe steatosis and presence of liver fibrosis (OR 3.02, 95%CI 1.46-6.26, P=0.003 and OR 2.18, 95%CI 1.03-4.60, P=0.041 respectively), and there was a non-significant but borderline increased risk of NASH (OR 2.00, 95%CI 0.98-4.06, P=0.056).Conclusions: Low ASM% in the presence of carriage of G allele of PNPLA3 rs738409 is associated with more severe NAFLD.
Microvascular changes and correlation with visual loss in pituitary adenoma
Authors: Chen Yaying , Li Xiaojiao , Song Xiangyuan , Cong Lin , Zhang Yuyan 
Published: May 2022

Abstract
Purpose To determine microvascular changes and the correlation between microvascular interception and optic nerve damage in pituitary adenoma (PA) patients.Methods The vessel density of optic disc and macular retinal vessels was measured, using optical coherence tomography angiography (OCTA). It was performed that correlation between microvascular interception and optic nerve damage, including visual field and structural measurements. Results Seventy four eyes from 40 pituitary adenoma patients and 36 eyes from 24 healthy voluntary controls were recruited prospectively. Optic disc vessel density from PA patients with visual field defect (perimetric PA) was reduced and macular vessel density at superficial retinal capillary plexus (SCP) level from both perimetric and preperimetric PA patients was increased, compared to controls. Optic retinal vessel density was inversely correlated with visual field mean deviation and positively correlated with retinal structural measurements. Conclusions The retinal microvascular changes in pituitary adenoma patients presented as significantly decreased optic vessel density in perimetric stage and significantly increased SCP macular vessel density in preperimetric stage. Optic retinal vessel density in PA patients was associated with advanced visual field damage and optic nerve structural impairments. OCTA seems to be a reliable method to detect retinal microvascular changes and access the severity of visual loss in chiasmal compression caused by pituitary adenoma.
The link between the consumption of dairy products with 10-year Framingham risk Score among women
Authors: Salehi Zahra , Namazi Nazli , Azadbakht Leila 
Published: May 2022

Abstract
Background
Given that in Iran women are more at risk of cardiovascular diseases (CVDs) than men, the aim of the present study was to investigate the consumption of dairy products in relation to Framingham risk Score (FRS) and cardiovascular risk factors in women.
Methods
371 women aged 18 to 50 years were recruited in this cross-sectional study. A validated and reliable food frequency questionnaire was used to measure diet. A 10-year odds of developing CVD between participants was predicted using FRS.
Results
Higher intake of total dairy, low-fat and high-fat dairy products was associated with higher FRS, but receiving each dairy, in particular, had nothing to do with FRS. Doogh consumption was directly associated with fasting blood sugar (FBS) (Odds ratio (OR): 14.11, 95% confidence interval (CI): 1.97, 101.03; P = 0.999). Also intake of high-fat dairy was directly correlated with serum levels of triglyceride (TAG) (OR: 4.47, 95% CI: 1.75, 11.43; P = 0.999). However, no correlation was observed between dairy products and other CVD risk factors.
Conclusions
Overall, consumption of doogh and high-fat dairy products was correlated with greater serum levels of FBS and TAG, respectively. Intake of total dairy, low-fat and high-fat dairy products was also associated with higher FRS. Future studies are needed to elucidate the link between dairy consumption and risk factors of CVD to characterize gender differences.
Programmable positive and negative chemotaxis of polymeric vesicles driven by degradation
Authors: Wang Danni , Luan Jiabin , Zhang Shaohua , White Paul , Wilson Daniela 
Published: May 2022

Abstract
Chemotaxis enables organisms and cells to move either toward or away from specific cues. Artificial protocells capable of integrating both positive and negative chemotaxis into one system are still very rare outside the biological realm and often require elaborately designed catalytic reactions with complex adaptation processes. Furthermore, the chemotactic mechanism of artificial systems is not yet fully investigated and understood especially at the molecular level. Here, we introduce degradation as a new propulsion mechanism for chemotactic polymeric vesicles without any external modification of the system. The vesicles can be programmed to exhibit either positive or negative chemotaxis by sensing the degrading catalyst as the attractant or the degradation products as the repellents. We systematically examined the possible mechanisms of the chemotaxis by a flow-chart toolbox approach and highlight the degradation-driven mechanism involved at the molecular level. The programmable chemotaxis driven by degradation represents a conceptually novel way to guide the design of artificial chemotactic protocells and opens a new route for biodegradable systems in fields such as the active delivery or artificial communication under out-of-equilibrium conditions.
Microwave-Assisted Digestion Using Diluted Nitric Acid for determination of nutrients elements in different waste fruit of agroindustry by inductively coupled plasma optical emission spectrometry (ICP OES)
Authors: Guedes Wesley Nascimento , Costa Vinicius Câmara , Santos Ingrid Alves , Tavares Iasnaia Maria de Carvalho , Pimetel Adriana Bispo , de Oliviera Julieta Rangel , Franco Marcelo 
Published: May 2022

Abstract
The current study describes an analytical procedure of microwave-assisted digestion using diluted nitric acid for determination nutrients elements in different waste fruit of agroindustry by inductively coupled plasma optical emission spectrometry (ICP OES). The optimization step was performed using the Doehlert matrix being that the studied variables were nitric acid concentration (2.0 mol L− 1) and hydrogen peroxide volume (3.0 mL). The accuracy of the proposed procedure was accessed by analysis of four certified reference material (CRM) of apple leaves (NIST 1515), spinach leaves (NIST 1570a), rice flour (NIST 1568a) and tomato leaves (NIST 1573a). The proposed procedure was successfully applied to the determination of Ca, Mg, K, Na, P, Fe, Zn, Mn and Cu in different waste fruit of agroindustry. Considering some critical parameters such as cost, green chemistry, and analyst safety, the proposed procedure has advantages compared to classical microwave-assisted digestion procedures based on concentrated acids.
Initiation of Renin-Angiotensin System Inhibitors and the Incidence of First Complete Remission in Patients with Primary Nephrotic Syndrome: A Nationwide Cohort Study
Authors: Shimizu Sayaka , Niihata Kakuya , Nishiwaki Hiroki , Shibagaki Yugo , Yamamoto Ryohei , Nitta Kosaku , Tsukamoto Tatsuo , Uchida Shunya , Takeda Asami , Okada Hirokazu , Narita Ichiei , Isaka Yoshitaka , Kurita Noriaki 
Published: May 2022

Abstract
Background
Renin-angiotensin system inhibitors (RASis) are recommended as the first-line therapy to reduce proteinuria in primary nephrotic syndrome. It is unclear whether RASis can induce complete remission (CR) in patients on immunosuppressive therapy.
Methods
This retrospective cohort study included adults with primary nephrotic syndrome from causes other than minimal change disease who were enrolled in the Japanese Nephrotic Syndrome Cohort Study from January 2009 to December 2010, with a 5-year follow-up period, and were not on RASis at enrolment. The exposure was a new RASi prescription, and the outcome was the first CR, defined as a urinary protein level < 0.3 g/day. Marginal structural models and Poisson regression were used to account for time-varying covariates and to estimate the causal effects of RASis on CR.
Results
Of the 84 eligible patients (median age, 65 years; males, 57%), 51 (61%) had membranous nephropathy. At baseline (1 month after renal biopsy and/or starting immunosuppressive therapy), 87% and 33% of patients were prescribed corticosteroids and calcineurin inhibitors, respectively. RASis were prescribed to 66% of patients during the study period. Sixty-five patients experienced CR during a median follow-up of 9.0 months (incidence remission rate, 5.05/100 person-months). RASi use was associated with a higher (adjusted incidence rate ratio [aIRR]: 2.27, 95% confidence interval [CI]: 1.06–4.84), and a lower (aIRR: 0.17, 95% CI: 0.04–0.68) CR rate in patients with membranous nephropathy and other pathologies, respectively.
Conclusion
RASis are beneficial as an adjuvant therapy for inducing remission in patients with membranous nephropathy.
COV-Score predicts mortality in SARS-CoV-2 infection
Authors: Lazar Mihai , Barbu Constanta Ecaterina , Chitu Cristina , Anghel Ana-Maria , Niculae Cristian , Manea Eliza , Damalan Anca , Bel Adela , Patrascu Raluca , Hristea Adriana , Ion Daniela 
Published: May 2022

Abstract
Purpose to present the importance of quantitative evaluation of radiological changes in SARS-CoV-2 pneumonia, including an alternative way to evaluate the lung involvement using normal density clusters. Based on these elements we have developed a more accurate new predictive score which includes quantitative radiological parameters. The current evolution models used in the evaluation of severe cases of COVID-19 include only qualitative or semi-quantitative evaluations of pulmonary lesions which lead to a less accurate prognosis and assessment of pulmonary involvement. Methods We performed a retrospective observational cohort study that included 100 adult patients admitted with confirmed severe COVID19. The patients were divided into two groups: group A (76 survivors) and group B (24 non-survivors). Results We found associated with higher mortality a lower percentage of normal lung densities, PaO2/FiO2 ratio, lymphocytes, platelets, hemoglobin and serum albumin, respectively a higher percentage of interstitial lesions, oxygen flow, FiO2, Neutrophils/lymphocytes ratio, lactate dehydrogenase, creatine kinase MB, myoglobin, and serum creatinine. The most accurate regression model included as predictors: age, lymphocytes, PaO2/FiO2 ratio, and percent of lung involvement, lactate dehydrogenase, serum albumin, D-dimers, oxygen flow, and myoglobin. Based on these parameters we developed a new score (COV-Score).Conclusion COV-Score represents a viable alternative to current prediction scores, demonstrating a better sensitivity and specificity in predicting a poor outcome at the time of admission. Quantitative assessment of lung lesions improves the prediction algorithms compared to the semi-quantitative parameters. The cluster evaluation algorithm increases the non-survivor and overall accuracy prediction.
Transforming growth factor-β1 as a biomarker in COPD patients with pulmonary fibrosis
Authors: Liu Jie , Wang Chun , Li Yongchun , Wang Jiahui , Hao Wanming , Li Qinghai , Zhu Wenjing , Han Wei 
Published: May 2022

Abstract
Background: TGF-β1 has been reported to be associated with COPD and pulmonary fibrosis, however, there is a lack of research on TGF-β1 in COPD patients with pulmonary fibrosis. In our study, we studied the influence of TGF-β1 and its single nucleotide polymorphism (SNP) on COPD and COPD complicated with pulmonary fibrosis. Methods: In this research, six GEO datasets were included to screen dysregulated genes in COPD patients. The dysregulated genes were detected by PCR-DNA sequencing based on 98 COPD patients and 90 healthy volunteers. Results: Five genes were upregulated in COPD patients. However, only TGF-β1 highly expressed in 1-4 stage of COPD versus 0 stage. Moreover, allele C of TGF-β1 +869 locus was associated with the susceptibility of COPD. The predicted value of FEV1% (FEV1, Forced Expiratory Volume in One Second) in patients with CC of +869 locus was significantly lower than that in patients with TT (P < 0.05). The genotype frequencies of CC, CT and TT were 6.5%, 58.7% and 34.8% in Mild-to-Moderate airflow restriction patients, however, they were 23.1%, 57.7% and 19.2% in severe airflow restriction patients. The distribution of CC genotype in severe airflow restriction COPD patients was significantly higher than that in Mild-to-Moderate airflow restriction COPD patients (P = 0.037). Further, the frequency of C allele was higher in patients with severe airflow restriction than that patients with Mild-to-Moderate airflow restriction (P = 0.024). Overall, TGF-β1 upregulated in COPD patients and its SNP at +869 is closely related to the susceptibility and airflow restriction of COPD patients.Conclusion: This study provides the basis for early prevention, targeted intervention and gene targeted therapy of COPD susceptible population.
Accuracy of gallbladder polyp size in predicting and detecting gallbladder cancer-a single-center study on gallbladder polyp
Authors: Liu Shuwang , Wang Yan , Zhang Lifen , Xu Jing , Lu Xiaofang , Wu Tianchong , Deng Lingna , Jiang Jiling , An Tailai 
Published: May 2022

Abstract
Background: Despite the fact that most gallbladder polyps are benign, malignant lesions are not so rare as thought. We performed this study to evaluate size distribution of gallbladder polyps and assess efficiency of reported risk factors in predicting malignancy. Methods: In this study, medical records of patients having undergone laparoscopic cholecystectomy for gallbladder polyps at Department of Hepatobiliary and Pancreatic Surgery, Shenzhen People,s Hospital were reviewed. Chi-square test and binary logistic regression analysis were adopted to determine risk factors for malignant lesions. Additionally, receiver operating curve (ROC) analysis was accomplished to identify the optimal cutoff size. Results: A total of 1012 patients were included in this study. Of the 1012 patients, 977 ones were diagnosed with benign gallbladder polyps while 35 ones with malignant polyps. Diameters of the 977 benign gallbladder polyps were between 1 and 50 mm while the 35 malignant polyps were between 4 and 54 mm. Incidence of gallbladder cancer among lesions ≥10mm was 15.5%, 31.5% for lesions ≥15mm and 49.0% for lesions ≥20mm. Among polyps in the following four size ranges (≤5mm, 6-9mm, 10-14mm, and 15-19mm), cholesterol polyps were the most common. While among polyps between 20 and 24mm, adenomas were the most common and among polyps ≥25mm, malignant tumors were the most common. Sessile morphology was significantly more common in malignant lesions (91.4%) than in benign lesions (3.7%, P<0.001). Multiple polyps were frequently diagnosed not only as cholesterol polyps (82%) but also as adenomas (4.3%) and gallbladder cancer (0.3%). We demonstrated that sessile morphology, blood flow signal on ultrasonography and single polyps were independent predictive factors for gallbladder malignancy. Conclusions: Detection rate of malignant lesions increased significantly with the increase in diameter. Risk factors such as sessile morphology, blood flow signal on ultrasonography and single polyps were effective factors predicting malignant lesions. It was difficult for us to accurately confirm the pathological diagnoses of gallbladder polyps solely based on preoperative examinations given the fact that cholesterol polyps and adenomas were quite common, even among large gallbladder polyps.
Self-adaptive Salp Swarm Algorithm for Optimization Problems
Authors: Kassaymeh Sofian , Abdullah Salwani , Al-Betar Mohammed , Alweshah Mohammed , Al-Laham Mohamad , Othman Zalinda 
Published: May 2022

Abstract
In this paper, an enhanced version of the Salp Swarm Algorithm (SSA) for global optimization problems was developed. Two improvements have been proposed: (i) diversification of the SSA population referred as SSAstd, (ii) SSA parameters are tuned using a self-adaptive technique based Genetic Algorithm (GA) referred as SSAGA−tuner. The novelty of developing a self-adaptive SSA is to enhance its performance through balancing search exploration and exploitation. The enhanced SSA versions are evaluated using twelve benchmark functions. The diversified population of SSAstd enhances convergence behavior, and self-adaptive parameter tuning of SSAGA−tuner improves the convergence behavior as well, thus improving performance. The comparative evaluation against nine well-established methods shows the superiority of the proposed SSA versions. The enhancement amount in accuracy was between 2.97% and 99% among all versions of algorithm. In a nutshell, the proposed SSA version shows a powerful enhancement that can be applied to a wide range of optimization problems.
Joint application of multiple genes in the diagnosis and pathogenesis of metastatic peochormocytoma/paraganglioma based on bioinformatics
Authors: Zhang Chunlei , Kang Yindong , Jiao Hongmei , Hu You , Chang Dehui , Yang Qi 
Published: May 2022

Abstract
Background: Metastatic pheochromocytomas and paragangliomas (PPGLs) are rare types of PPGLs with a worse prognosis. However, there are no effective diagnostic molecular markers and the specific mechanism is still unclear. In this study, we screened the molecular diagnostic markers and predicted the metastasis mechanism of metastatic PPGLs through mRNA sequencing results in the Gene Expression Omnibus (GEO) dataset GSE67066 by bioinformatics. Methods and results: Data were obtained from GEO dataset GSE67066. R software and packages were used for differentially expressed genes (DEGs) analysis, receiver operating characteristic curve (ROC) assessment, logistic model construction, correlation analysis. Gene Data in The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) database were included in the pan-cancer analysis. Finally, 35 up-regulated genes and 38 down-regulated genes were identified. All malignant tumors can be distinguished from the benign group by logistics regression equation (= 957.8354 + 49.148*IRX3 + -14.1748*GALNT10 + -30.2242*BAIAP3 + -34.7723*RAPGEF4 + -9.3218*PACRG + -51.3402*PRKCZ + 7.5959*CAMK2N1 + -43.7049*RD3). CNTN4, PAPPA, LPL, TG, FGF14, OPCT, ABAT, GSTO2, SLC24A2, SGSM1, ATCAY and BAIAP3 may be important genes for the metastasis of PPGLs through NORCH signaling and TNF/HIF1α/VEGF signaling pathways. KCNG3, PXMP2, SHROOM3, KIF18B and FAM49A expression was related to mutation types. Many DEGs’ expression presented the anti-tumor potential correlated with immunity checkpoints in the metastatic process of PPGLs.Conclusions: Combined gene mRNA expression model increases the diagnostic efficiency of the metastatic potential of PPGLs. Multiple genes including CNTN4, PAPPA, LPL, TG, FGF14, OPCT, ABAT, GSTO2, SLC24A2, SGSM1, ATCAY and BAIAP3 may participate in the metastasis of PPGLs through NORCH signaling and TNF/HIF1α/VEGF signaling pathways.