bluuebunny/crossref_metadata_2025_split huggingface dataset:
Основні сучасні теоретичні аспекти взаємозв’язку економічного потенціалу та економічної могутності держави
Authors: Semenenko Oleh , Onofriichuk Petro , Vodchyts Oleksandr , Taran Olha , Cherevatyi Taras , Romanchenko Olha 
Published: November 2022

Мета роботи: є розкриття основних сучасних теоретичних аспектів та особливостей у взаємозв’язку діалектичних понять економічного потенціалу та економічної могутності держави в рамках сьогодення.
Дизайн/Метод/Підхід дослідження: основними методами досліджень є діалектичні методи, аналізу та синтезу економічних процесів, методи економічної теорії, методи економічної логіки.
Результати дослідження: розкриті сутності та зміст понять економічного потенціалу та економічної могутності держави, визначено теоретичні погляди щодо їх взаємозв’язку один з одним, розкрито можливі шляхи підвищення ефективності нарощування економічного потенціалу та економічної могутності держави.
Теоретична цінність дослідження: основними результатами досліджень за тематикою статті є: розкриття основних сучасних аспектів взаємозв’язку економічного потенціалу та економічної могутності держави, розкрито їх структурні елементи та сформована залежність між ними, а також визначено погляди щодо подальшого дослідження висвітлених у статті питань.
Тип статті: описовий, теоретичний, діалектичний.
Про завдання органів досудового розслідування в умовах подолання наслідків агресії рф на деокупованих територіях: до постановки проблеми
Authors: Бондар В.С. , Яковенко М. О. 
Published: November 2022

Стаття присвячена діяльності органів досудового розслідування в умовах подолання наслідків агресії рф на деокупованих територіях. Розглянуто ключові аспекти організації діяльності підрозділів слідства та дізнання в частині проведення стабілізаційних заходів. Проаналізовано нормативне регулювання відновлення втрачених матеріалів кримінального провадження. Розроблено відповідні організаційно-тактичні рекомендації, а також теоретичні положення, спрямовані на вдосконалення нормативно-правового регулювання досліджуваного інституту. Проаналізовано ефективність запроваджених змін до Кримінального процесуального кодексу України щодо удосконалення порядку здійснення кримінального провадження в умовах воєнного стану. Констатовано, що існуючий механізм відновлення втрачених матеріалів кримінального провадження та діяльності органів досудового розслідування на деокупованих територіях потребує вдосконалення. Висвітлено питання взаємодії органів досудового розслідування з підрозділами, які здійснюють оперативно-розшукову діяльність. Систематизовано процесуальні та організаційні форми взаємодії слідчого з оперативними підрозділами з урахуванням особливостей досудового розслідування, що провадиться в умовах воєнного стану. Обґрунтовано, що під час відновлення кримінальної юстиції на звільнених територіях, крім слідчо-оперативних груп, мають бути створені спеціальні інвентаризаційні комісії, до складу яких можуть входити працівники органів прокуратури (процесуальні керівники), керівники органів досудового розслідування (їх заступники), представники органів Державного бюро розслідувань, працівники підрозділів інформаційно-аналітичного забезпечення та режимно-секретних відділів. Розкрито питання щодо забезпечення принципу невідворотності покарання та розслідування кримінальних правопорушень, що вчинялися в період окупації як з боку комбатантів військ російської федерації, так і звичайними місцевими мешканцями. Проаналізовано особливості обстеження приміщень, у яких розташовувалися так звані «органи поліції» чи «комендатури». Охарактеризовано значення документів, виданих окупаційною владою, як джерел криміналістично значущої інформації.Р озглянуто аспекти, пов’язані з проведенням перевірок щодо причин смерті людей, померлих у період тимчасової окупації. Запропоновано залучення представників закладів охорони здоров’я та спеціальних груп, які виконують гуманітарну місію, спрямовану на вилучення тіл (останків) померлих (загиблих).
PERANAN ORGANISASI KEPRAMUKAAN DALAM MENGEMBANGKAN KARAKTER, SEMANGAT KEBANGSAAN DAN KETERAMPILAN HIDUP SISWA DI SMA NEGERI 1 KULISUSU
Authors: Nurlaila Nurlaila 
Published: November 2022

Tujuan penelitian adalah untuk mendeskripsikan dan menganalisis Peran organisasi kepramukaan dalam mengembangan semagat kebangsaan siswa SMA Negeri 1 Kulisusu di Kabupaten Buton Utara. Penelitian ini menggunakan metode penelitian kualitatif dengan desain studi kasus. Penentuan subjek penelitian menggunakan teknik purposive yaiatu kepala sekolah, guru, para pembina pramuka, dan siswa di SMAN 1 Kulisusu. Pengumpulan data menggunakan teknik observasi, wawancara, dan dokumen. Hasil penelitian menunjukkan bahwa: (1) Peran organisasi kepramukaan dalam mengembangkan karakter siswa SMA Negeri 1 Kulisusu, yaitu (a) sebagai ekstrakulikuler wajib. (b) Golongan Pramuka Penegak dijalankan dengan fokus penempuhan SKU dan (c) Kegiatan Pramuka sebagai sarana pembentukan karakter. (2) Peran organisasi kepramukaan mengembangan semagat kebangsaan siswa SMA Negeri 1 Kulisusu di Kabupaten Buton Utara dilakukan melalui keteladanan, penegakan kedisiplinan, penugasan, pembiasaan, ceramah, dan permainan edukatif. (3) Peran organisasi kepramukaan membekali keterampilan hidup siswa SMA Negeri 1 Kulisusu di Kabupaten Buton Utara yaitu: (a) Pengamalan kode kehormatan gerakan pramuka. Kode kehormatan pramuka untuk golongan penegak terdiri atas janji yang disebut Tri Satya dan ketentuan moral yang disebut Dasa Dharma. (b) Belajar sambil melakukan (learning by doing). (c) Sistem beregu (patrol system). (d) Kegiatan di alam terbuka. (e) Kemitraan dengan anggota dewasa dalam setiap kegiatan. (f) Sistem satuan terpisah untuk putra dan putri. (f) Sistem among. (g) Rekreasi dan mengisi waktu dengan kegiatan yang positif.Kata Kunci: Kepramukaan; Pengembangan Karakter
Synthesis of activity evaluation of flavonoid derivatives as ɑ-glucosidase inhibitors
Authors: Zhu Hua , Zhong Xin 
Published: November 2022

Six flavonoid derivatives were synthesized and tested for anti-α-glucosidase activities. All derivatives were confirmed using NMR and HRMS and exhibited excellent inhibitory effects on α-glucosidase. Derivative four exhibited the highest anti-α-glucosidase activity (IC50: 15.71 ± 0.21 μM). Structure-activity relationship results showed that bromine group would be the most beneficial group to anti-α-glucosidase activity. Inhibitory mechnism and inhibition kinetics results showed derivative four was a reversible and mixed-type inhibitor. Molecular docking revealed that derivative four was tightly bind to the amino acid residues of active pocket of α-glucosidase and formed hydrogen bond, π-π stacking, and Pi-Donor hydrogen with α-glucosidase. Moreover, the physicochemical parameters of all derivatives were assessed using SwissADME software. This results also showed that the hybridization of flavonoid and phenylpropionic acid would be a useful strategy for the development of α-glucosidase inhibitors.
Clinical features and metabolic reprogramming of atherosclerotic lesions in patients with chronic thromboembolic pulmonary hypertension
Authors: Liu Jixiang , Chang Ziyi , Zhang Zhu , Wang Bei , Xie Wanmu , Gao Qian , Zhang Shuai , Zhang Yunxia , Tian Han , Fu Zhihui , Li Yishan , Zhen Kaiyuan , Ma Shuangshuang , Zhong Dingrong , Yang Peiran , Zhai Zhenguo 
Published: November 2022

BackgroundChronic thromboembolic pulmonary hypertension (CTEPH) patients may present with atherosclerotic lesions in their pulmonary arteries, but their clinical characteristics remain unclear. The metabolic pathways associated with the atherosclerotic lesions may explain their occurrence and have implications for interventions, but they have not been investigated.MethodsWe collected pulmonary endarterectomy (PEA) samples of CTEPH patients from December 2016 to August 2021. Following a detailed pathological examination of the PEA specimen, the patients were divided into those with and without lesions, and age- and sex matching were performed subsequently using propensity score matching (n = 25 each). Metabolomic profiling was used to investigate the metabolites of the proximal lesions in the PEA specimens.ResultsIn our study population, 27.2% of all PEA specimens were found to contain atherosclerotic lesions. CTEPH patients with atherosclerotic lesions were more likely to have a history of symptomatic embolism and had a longer timespan between embolism and surgery, whereas the classic risk factors of systemic and coronary circulation could not distinguish CTEPH patients with or without atherosclerotic lesions. Metabolomic profiling revealed that the formation of atherosclerotic lesions in CTEPH was closely related to altered glycine, serine, and threonine metabolic axes, possibly involved in cellular senescence, energy metabolism, and a proinflammatory microenvironment.ConclusionThe occurrence of atherosclerotic lesions in the pulmonary arteries of CTEPH was associated with symptomatic thromboembolic history and prolonged disease duration. The results revealed a new link between atherosclerotic lesions and aberrant amino acid metabolism in the context of CTEPH for the first time. This study has characterized the clinical and metabolic profiles of this distinct group of CTEPH patients, providing new insights into disease pathogenesis and potential interventions.
Development of a 3D printed surgical guide for Brugada syndrome substrate ablation
Authors: Talevi Giacomo , Pannone Luigi , Monaco Cinzia , Bori Edoardo , Cappello Ida Anna , Candelari Mara , Ramak Robbert , La Meir Mark , Gharaviri Ali , Chierchia Gian Battista , Innocenti Bernardo , de Asmundis Carlo 
Published: November 2022

BackgroundBrugada syndrome (BrS) is a disease associated with ventricular arrhythmias and sudden cardiac death. Epicardial ablation has demonstrated high therapeutic efficacy in preventing ventricular arrhythmias. The purpose of this research is to define a workflow to create a patient-specific 3D-printed tool to be used as a surgical guide for epicardial ablation in BrS.MethodsDue to their mechanical properties and biocompatibility, the MED625FLX and TPU95A were used for cardiac 3D surgical guide printing. ECG imaging was used to define the target region on the right ventricular outflow tract (RVOT). CT scan imaging was used to design the model based on patient anatomy. A 3D patient-specific heart phantom was also printed for fitting test. Sterilization test was finally performed.Results3D printed surgical models with both TPU95A and MED625FLX models were in agreement with pre-specified imputed measurements. The phantom test showed retention of shape and correct fitting of the surgical tool to the reproduced phantom anatomy, as expected, for both materials. The surgical guide adapted to both the RVOT and the left anterior descending artery. Two of the 3D models produced in MED265FLX showed damage due to the sterilization process.ConclusionsA 3D printed patient-specific surgical guide for epicardial substrate ablation in BrS is feasible if a specific workflow is followed. The design of the 3D surgical guide ensures proper fitting on the heart phantom with good stability. Further investigations for clinical use are eagerly awaited.
Is there a secular trend regarding puberty in children with down syndrome?
Authors: Erdoğan Furkan , Güven Ayla 
Published: November 2022

IntroductionThere are very few studies on the age of onset and end of puberty in children with Down syndrome (DS). Also, data regarding the course of puberty in these children compared to their healthy peers is limited. Moreover, there is limited information regarding the effects of factors such as obesity and hypothyroidism on the puberty process in children with DS. Our aim in our study is to determine whether the pubertal development of children with DS differs from their healthy peers and from previous studies conducted with DS children.MethodsThe medical records of DS children were examined retrospectively. The anthropometric measurements and the age of onset of pubertal stages, and menarche were recorded. The patients’ age at puberty onset, the puberty processes, and age at menarche were compared with their healthy peers and previously published data on children with DS.ResultsOf the 140 Down syndrome patients followed in our clinic, 51 of whom with puberty constituted the study group. The mean age of onset of puberty was 10.3 ± 1.0 years in our group (10.0 ± 0.8 years for girls, 10.6 ± 1.2 years for boys, respectively). Obesity occurred in 46% of pubertal girls with DS. The age of menarche in girls with DS was 11.8 ± 0.7 years. The menarche age of girls with DS was significantly different from healthy girls. In the DS boys, only the Tanner V stage ages were different from the healthy children. True- precocious-puberty was detected in three children.ConclusionAlthough breast development begins later in females with DS than in their healthy peers; menarche is detected earlier than in their peers and a tendency towards obesity in the whole population. While the age of pubertal onset was similar to healthy children in male patients, our findings suggest that their puberty duration is longer.
Analysis of relative factors and prediction model for optimal ovarian response with gonadotropin-releasing hormone antagonist protocol
Authors: Jiang Wenwen , Zheng Beihong , Liao Xiuhua , Chen Xiaojing , Zhu Suqin , Li Rongshan , Zhang Huale 
Published: November 2022

ObjectiveTo explore the relative factors for best ovarian response in patients undergoing assisted reproductive technology with the gonadotropin-releasing hormone antagonist protocol and to establish a nomogram prediction model of ovarian response.MethodsA retrospective cohort analysis of the clinical data of 1,944 patients who received assisted reproductive treatment in the Center for Reproductive Medicine of Fujian Maternity and Child Health Hospital from April 1, 2018, to June 30, 2020. According to the number of oocytes obtained, there were 659 cases in the low ovarian response group (no more than five oocytes were retrieved), 920 cases in the normal ovarian response group (the number of retrieved oocytes was &gt;5 but ≤18), and 365 cases in the high ovarian response group (&gt;18 oocytes retrieved). Independent factors affecting ovarian responsiveness were screened by logistic regression, which were the model entry variables, and a nomogram prediction model was established based on the regression coefficients.ResultsThere were statistically significant differences in age, anti-Mullerian hormone, antral follicle count, the diagnosis of endometriosis, decreased ovarian reserve, polycystic ovary syndrome, basal follicle-stimulating hormone and basal luteinizing hormone among the three groups (P &lt; 0.001). Multifactorial stepwise regression analysis showed that female age (0.95 [0.92–0.97], P = 0.000), decreased ovarian reserve (0.27 [0.19-0.38]), P = 0.000), endometriosis (0.81 [0.56-0.86], P = 0.000), antral follicle count (1.09 [1.06-1.12], P = 0.000), basal follicle-stimulating hormone (0.90 [0.85-0.96], P = 0.001), Anti-Mullerian hormone (1.19 [1.13–1.26], P= 0.000) and luteinizing hormone on trigger day (0.73 [0.66–0.80], P= 0.000), were independent factors for the occurrence of different ovarian responses during ovarian hyperstimulation. The predictive model of ovarian responsiveness was constructed based on the above factors, and the model was verified with 589 patients’ data from July 1, 2020, to December 31, 2020, at this center. The predicted ovarian response (number of eggs obtained) of a total of 450 patients was consistent with the actual results, with a coincidence degree of 76.4%, and the consistency index of the model is 0.77.ConclusionThe nomogram model was successfully developed to effectively, intuitively, and visually predict the ovary reactivity in the gonadotropin-releasing hormone antagonist protocol and provide guidance for clinical practice.
Case report: Autosomal recessive bestrophinopathy with macular cysts and MNV over 13-year follow-up
Authors: Zhang Lei , Wang Hai-Yan , Jia Wei , Wang Ru , Wang Yu-Sheng , Cui Yang-Yang 
Published: November 2022

Purpose: To describe the phenotype and genotype of a patient with autosomal recessive bestrophinopathy (ARB) over a 13-year follow-up period.Methods: The phenotype of the subject was described after a complete ophthalmological examination, which included fundus photography, optical coherence tomography (OCT), fundus autofluorescence, fluorescein angiography (FA), indocyanine green angiography (ICGA), electroretinogram (EOG), electroretinography (ERG), and multifocal electroretinogram (mfERG). Genetic analyses were carried out by screening the variations via whole-exome sequencing.Results: This patient presented with retinoschisis and cystic changes when he was 7 years old and was diagnosed with X-linked retinoschisis. In the 13th year after the first presentation, enlarged macular cysts with retinoschisis, macular neovascularization (MNV), and subretinal fluid were displayed on OCT. Autofluorescence showed hyperfluorescence corresponding to the area of retinal pigment epithelium (RPE) change. EOG showed no light peak, and the Arden ratio was less than 2.0. Whole-exome sequencing revealed compound heterozygous sequence variations (p. [Arg47Leu; Trp287*]) in the coding sequence of the BEST1 allele inherited from his parents. Thus, a diagnosis of ARB combined with secondary MNV was made.Conclusion: Patients with compound heterozygous BEST1 mutations developed ARB, which could show significant retinoschisis at a young age. Genetic analyses, autofluorescence, and EOG are essential to diagnose ARB correctly in consequence of considerable phenotypic variations.
Comprehensive analyses of the microRNA–messenger RNA–transcription factor regulatory network in mouse and human renal fibrosis
Authors: Deng Le , Xu Gaosi , Huang Qipeng 
Published: November 2022

Objective: The aim of this study was to construct a microRNA (miRNA)–messenger RNA (mRNA)–transcription factor (TF) regulatory network and explore underlying molecular mechanisms, effective biomarkers, and drugs in renal fibrosis (RF).Methods: A total of six datasets were downloaded from Gene Expression Omnibus. “Limma” and “DESeq2” packages in R software and GEO2R were applied to identify the differentially expressed miRNAs and mRNAs (DEmiRNAs and DEmRNAs, respectively). The determination and verification of DEmiRNAs and DEmRNAs were performed through the integrated analysis of datasets from five mouse 7 days of unilateral ureteral obstruction datasets and one human chronic kidney disease dataset and the Human Protein Atlas (http://www.proteinatlas.org). Target mRNAs of DEmiRNAs and TFs were predicted by prediction databases and the iRegulon plugin in Cytoscape, respectively. A protein–protein interaction network was constructed using STRING, Cytoscape v3.9.1, and CytoNCA. Functional enrichment analysis was performed by DIANA-miRPath v3.0 and R package “clusterProfiler.” A miRNA–mRNA–TF network was established using Cytoscape. Receiver operating characteristic (ROC) curve analysis was used to examine the diagnostic value of the key hub genes. Finally, the Comparative Toxicogenomics Database and Drug-Gene Interaction database were applied to identify potential drugs.Results: Here, 4 DEmiRNAs and 11 hub genes were determined and confirmed in five mouse datasets, of which Bckdha and Vegfa were further verified in one human dataset and HPA, respectively. Moreover, Bckdha and Vegfa were also predicted by miR-125a-3p and miR-199a-5p, respectively, in humans as in mice. The sequences of miR-125a-3p and miR-199a-5p in mice were identical to those in humans. A total of 6 TFs were predicted to regulate Bckdha and Vegfa across mice and humans; then, a miRNA–mRNA–TF regulatory network was built. Subsequently, ROC curve analysis showed that the area under the curve value of Vegfa was 0.825 (p = 0.002). Finally, enalapril was identified to target Vegfa for RF therapy.Conclusion: Pax2, Pax5, Sp1, Sp2, Sp3, and Sp4 together with Bckdha-dependent miR-125a-3p/Vegfa-dependent miR-199a-5p formed a co-regulatory network enabling Bckdha/Vegfa to be tightly controlled in the underlying pathogenesis of RF across mice and humans. Vegfa could act as a potential novel diagnostic marker and might be targeted by enalapril for RF therapy.
Age-associated global DNA hypermethylation augments the sensitivity of hearts towards ischemia-reperfusion injury
Authors: Boovarahan Sri Rahavi , Ali Nemat , AlAsmari Abdullah F. , Alameen Alaa Alnoor , Khan Rehan , Kurian Gino A. 
Published: November 2022

Most pre-clinical studies in cardiac ischemia-reperfusion injury (I/R) are carried out in young or old animals, which does not cater to the adult age in humans who encounter I/R. Not many studies in the literature are available that emphasize the sensitivity of the adult heart to injury from the young heart, where there exist distinct alterations in DNA methylation and mitochondrial function that contribute to injury. In the present study, we utilized young (8 weeks old) and adult (24 weeks old) rat hearts to evaluate distinct DNA methylation alterations that contribute to I/R injury. The cardiac basal physiological activities in young and adult rat hearts were insignificantly changed from normal. But the DNA hypermethylation and expression level of mitochondrial genes were slightly higher in adult rat hearts. The consequential effect of these changes was measured in the I/R heart to understand its response to additional stress. Accordingly, we noted an increase in global DNA hypermethylation levels by 40% and 62% in young and adult I/R hearts, respectively, from their respective control. Subsequently, a decline in mitochondrial genes (ND1, ND4L, ND6, Cyt B, COX1, COX2, and ATP8) that regulate cardiac contractility was observed in adult I/R hearts. These changes, in turn, reduced hemodynamics (Rate pressure product) by 51% and 32% in adult and young I/R hearts, respectively, from their controls. Besides, the I/R-linked infarct size was higher in adult hearts (58%) than in young hearts (37%). Correlation analysis showed a significant negative correlation of global DNA methylation with the MT-ND1 expression (r = −0.7591), MFN2 expression (r = −0.8561) and cardiac RPP (r = −0.8015) in adult I/R hearts. Based on the above observations, we concluded that age promoted DNA methylation and deteriorated cardiac responsive ability to resist I/R injury.