bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zelquistinel Is an Orally Bioavailable Novel NMDA Receptor Allosteric Modulator That Exhibits Rapid and Sustained Antidepressant-Like Effects
Authors: Burgdorf Jeffrey S , Zhang Xiao-Lei , Stanton Patric K , Moskal Joseph R , Donello John E 
Published: July 2022

Abstract

Background
The role of glutamatergic receptors in major depressive disorder continues to be of great interest for therapeutic development. Recent studies suggest that both negative and positive modulation of N-methyl-D-aspartate receptors (NMDAR) can produce rapid antidepressant effects. Here we report that zelquistinel, a novel NMDAR allosteric modulator, exhibits high oral bioavailability and dose-proportional exposures in plasma and the central nervous system and produces rapid and sustained antidepressant-like effects in rodents by enhancing activity-dependent, long-term synaptic plasticity.

Methods
NMDAR-mediated functional activity was measured in cultured rat brain cortical neurons (calcium imaging), hNR2A or B subtype-expressing HEK cells, and synaptic plasticity in rat hippocampal and medial prefrontal cortex slices in vitro. Pharmacokinetics were evaluated in rats following oral administration. Antidepressant-like effects were assessed in the rat forced swim test and the chronic social deficit mouse model. Target engagement and the safety/tolerability profile was assessed using phencyclidine-induced hyperlocomotion and rotarod rodent models.

Results
Following a single oral dose, zelquistinel (0.1–100 µg/kg) produced rapid and sustained antidepressant-like effects in the rodent depression models. Brain/ cerebrospinal fluid concentrations associated with zelquistinel antidepressant-like activity also increased NMDAR function and rapidly and persistently enhanced activity-dependent synaptic plasticity (long-term potentiation), suggesting that zelquistinel produces antidepressant-like effects by enhancing NMDAR function and synaptic plasticity. Furthermore, Zelquistinel inhibited phencyclidine (an NMDAR antagonist)-induced hyperlocomotion and did not impact rotarod performance.

Conclusions
Zelquistinel produces rapid and sustained antidepressant effects by positively modulating the NMDARs, thereby enhancing long-term potentiation of synaptic transmission.
Asymptotic solutions of the SIR and SEIR models well above the epidemic threshold
Authors: Kozyreff Gregory 
Published: July 2022

Abstract
A simple and explicit expression of the solution of the SIR epidemiological model of Kermack and McKendrick is constructed in the asymptotic limit of large basic reproduction numbers ${\mathsf R_0}$. The proposed formula yields good qualitative agreement already when ${\mathsf R_0}\geq 3$ and rapidly becomes quantitatively accurate as larger values of ${\mathsf R_0}$ are assumed. The derivation is based on the method of matched asymptotic expansions, which exploits the fact that the exponential growing phase and the eventual recession of the outbreak occur on distinct time scales. From the newly derived solution, an analytical estimate of the time separating the first inflexion point of the epidemic curve from the peak of infections is given. Finally, we use the same method on the SEIR model and find that the inclusion of the ‘exposed’ population in the model can dramatically alter the time scales of the outbreak.
Framework for integrating electronic patient-reported data in routine cancer care: an Oncology Intake Questionnaire
Authors: McCleary Nadine J , Haakenstad Ellana K , Cleveland Jessica L F , Manni Michael , Hassett Michael J , Schrag Deb 
Published: July 2022

Abstract

Objective
As part of ongoing implementation of electronic patient-reported outcome tools at the Dana-Farber Cancer Institute, here we describe the development of the electronic New Patient Intake Questionnaire.

Materials and Methods
The original New Patient Intake Questionnaire includes a review of symptoms, oncology history, family history, health behaviors, health and social status, health literacy and numeracy, which was modified for integration into the EHR using content determination, build and configuration, implementation, analytics, and interventions. The engagement of key stakeholders, including patients, clinical staff, and providers, throughout the development and deployment of the electronic Questionnaire was crucial to producing a successful tool. Continual modifications based on input of stakeholders (such as mode of tool deployment) were made to ensure the utility and usability of the tool for both patients and providers.

Results
Implementation of the EHR-integrated electronic New Patient Intake Questionnaire improved collection of the PRD by increasing questionnaire accessibility for patients, while also providing all available data to clinicians and researchers. Careful consideration of the content and configuration of the questionnaire allowed for a successful, institute-wide implementation of the tool.

Discussion
This effort demonstrates the feasibility of implementation of a system-wide electronic questionnaire, emphasizing the importance of iterative refinement to create a tool that is both patient-centric and usable for clinicians.

Conclusions
The electronic New Patient Intake Questionnaire allows for systematic collection of the PRD, which should benefit cancer care outcomes through innovative care delivery and healthcare interventions.
Sanitizing Equipment and Personnel to Prevent the Spread of Hibiscus Mealybug [Nipaecoccus viridis (Hemiptera: Pseudococcidae)] in Florida Citrus
Authors: Middleton Eric G , Diepenbrock Lauren M 
Published: July 2022

Abstract
Hibiscus mealybug (Nipaecoccus viridis, Newstead) is an emerging and serious pest of citrus in Florida. Preventing the spread of hibiscus mealybug by sanitizing personnel and equipment such as individual protective covers can help limit new infestations. Two mealybug life stages, first instars (crawlers) and eggs inside ovisacs, were tested as they are the most likely to be accidentally transported on people and tools. Isopropanol sprays in 3 commercially available concentrations (50%, 70%, and 90%) were tested against crawlers and ovisacs, and steam treatments and hot water immersion were tested against ovisacs. All concentrations of isopropanol resulted in &gt;90% incapacitation of mealybug crawlers, especially when two sprays of isopropanol were applied. However, no concentration of isopropanol reliably killed all mealybug eggs within ovisacs. Steam treatments and hot water immersion for 10 min or longer at 49°C and for 5 min or longer at 54.5°C resulted in 100% mortality of mealybug adults and eggs inside ovisacs. However, adults and ovisacs insulated inside of individual protective covers did not experience 100% mortality until 30 min or longer at 49°C, 10 min or longer at 54.5°C, and 5 min or longer at 60°C for steam treatments. Isopropanol sprays can effectively incapacitate the majority of hibiscus mealybug crawlers on personnel and tools, but are not effective against ovisacs. Steam treatments and hot water immersion can reliably lead to 100% mortality of ovisacs, but longer times and higher temperatures must be used for steam treatments if ovisacs are insulated by equipment.
New Identification Tools for the Nymphs of Three Amblyomma spp. Koch (Ixodida: Ixodidae) in South Texas
Authors: Alfred J T , Mertins J W 
Published: July 2022

Abstract
Taxonomic uncertainties and morphologic and molecular similarities may lead to incorrect or incomplete species identifications of immature ticks. This problem can be compounded when the diagnostic morphological features used are unreliable, unstable, or difficult to view. Our study attempts to sort out the similar morphologies of three Amblyomma spp. nymphs with partially overlapping geographic distributions in South Texas. Amblyomma americanum (L.), Amblyomma mixtum Koch, and Amblyomma tenellum Koch are three closely related species with similar nymphs and larvae. Previous attempts to morphologically distinguish among nymphs of these species have relied on difficult-to-observe and unreliable characters. Over the course of decades of routine diagnostic work with these ticks, we have observed and discovered consistent and useful morphologic features sufficient to practically distinguish among these nymphs. We confirmed our morphological studies with molecular genetic evidence (i.e., 12S mitochondrial genes), and we propose that practical, rapid morphological species-level identification of nymphs of the three named species is now possible with confidence.
MTH-3 sensitizes oral cancer cells to cisplatin via regulating TFEB
Authors: Tsai Shih-Chang , Yang Jai-Sing , Lu Chi‑Cheng , Tsai Fuu-Jen , Chiu Yu‑Jen , Kuo Sheng-Chu 
Published: July 2022

Abstract

Objectives
MTH-3, a curcumin derivative, exhibits improved water solubility. This study aims to elucidate the mechanisms underlying the anticancer effects of MTH-3 on human oral squamous cell carcinoma CAL27 cisplatin-resistant (CAR) cells.

Methods
To evaluate the biological functions of MTH-3 in CAR cells, flow cytometry, staining, and western blot analyses were used.

Key findings
MTH-3 reduced CAR cell viability and significantly induced autophagy in the presence of 10 and 20 μM MTH-3. Transcription factor EB was identified as the potential target of MTH-3. Autophagy-related proteins were upregulated after 24 h of MTH-3 incubation. MTH-3 treatment increased caspase-3 and caspase-9 enzyme activities. Mitochondrial membrane potential was decreased after MTH-3 treatment. MTH-3 triggered the intrinsic apoptotic pathway.

Conclusions
MTH-3 induces autophagy and apoptosis of CAR cells via TFEB. MTH-3 might be an effective pharmacological agent for treating oral cancer cells.
Military Resident Physician Training Burden: Quantifying Requirements Across the Military Health Care System
Authors: Medvescek Eli D , Foglia Lisa M , Thagard Andrew S 
Published: July 2022

ABSTRACT

Introduction
The Military Health Care System trains approximately 1,500 resident physicians in over 100 specialties. In addition to requirements for their specific program, active duty military trainees must complete military-specific trainings that vary by the branch of service. Excessive training requirements could contribute to physician burnout and/or negatively affect patient care. Therefore, the objective of this study was to quantify the time active duty resident physicians dedicate to this training, stratified by the branch of service.

Materials and Methods
The study protocol was submitted to the Clinical Investigations Department at Naval Medical Center Portsmouth (Portsmouth, VA, USA) and deemed exempt from the Institutional Review Board review. We conducted a descriptive study in 2021 wherein lists of all training requirements were obtained from a military treatment facility in the Army, Navy, and Air Force supporting residency training. Individual requirements were reviewed and sorted into military-specific and general categories. Information was gathered on duration, frequency, and platform for applicable requirements.

Results
Residents are required to complete a mean of 17.2 hours of training annually, of which 11.2 hours were military-specific. This consisted of 50, 57, and 53 individual requirements for Army, Navy, and Air Force personnel, respectively. Army resident physicians had the greatest time burden of military-specific training at 14.8 hours/year, followed by the Air Force and Navy (10.2 and 8.7 hours/year, respectively).

Conclusions
Annually, active duty resident physicians spend the equivalent of more than two work days completing additional training requirements on multiple platforms. Standardizing training requirements and platforms across the Military Health Care System and aligning required trainings with job responsibilities could free up additional time for patient care, potentially decreasing fatigue and burnout.
The positive evidence bias in perceptual confidence is unlikely post-decisional
Authors: Samaha Jason , Denison Rachel 
Published: July 2022

Abstract
Confidence in a perceptual decision is a subjective estimate of the accuracy of one’s choice. As such, confidence is thought to be an important computation for a variety of cognitive and perceptual processes, and it features heavily in theorizing about conscious access to perceptual states. Recent experiments have revealed a “positive evidence bias” (PEB) in the computations underlying confidence reports. A PEB occurs when confidence, unlike objective choice, overweights the evidence for the correct (or chosen) option, relative to evidence against the correct (or chosen) option. Accordingly, in a perceptual task, appropriate stimulus conditions can be arranged that produce selective changes in confidence reports but no changes in accuracy. Although the PEB is generally assumed to reflect the observer’s perceptual and/or decision processes, post-decisional accounts have not been ruled out. We therefore asked whether the PEB persisted under novel conditions that addressed two possible post-decisional accounts: (i) post-decision evidence accumulation that contributes to a confidence report solicited after the perceptual choice and (ii) a memory bias that emerges in the delay between the stimulus offset and the confidence report. We found that even when the stimulus remained on the screen until observers responded, and when observers reported their choice and confidence simultaneously, the PEB still emerged. Signal detection-based modeling showed that the PEB was not associated with changes to metacognitive efficiency, but rather to confidence criteria. The data show that memory biases cannot explain the PEB and provide evidence against a post-decision evidence accumulation account, bolstering the idea that the PEB is perceptual or decisional in nature.
Real-time PACS-integrated longitudinal brain metastasis tracking tool provides comprehensive assessment of treatment response to radiosurgery
Authors: Cassinelli Petersen Gabriel , Bousabarah Khaled , Verma Tej , von Reppert Marc , Jekel Leon , Gordem Ayyuce , Jang Benjamin , Merkaj Sara , Abi Fadel Sandra , Owens Randy , Omuro Antonio , Chiang Veronica , Ikuta Ichiro , Lin MingDe , Aboian Mariam S 
Published: July 2022

AbstractBackgroundTreatment of brain metastases can be tailored to individual lesions with treatments such as stereotactic radiosurgery. Accurate surveillance of lesions is a prerequisite but challenging in patients with multiple lesions and prior imaging studies, in a process that is laborious and time consuming. We aimed to longitudinally track several lesions using a PACS-integrated lesion tracking tool (LTT) to evaluate the efficiency of a PACS-integrated lesion tracking workflow, and characterize the prevalence of heterogenous response (HeR) to treatment after Gamma Knife (GK).MethodsWe selected a group of brain metastases patients treated with GK at our institution. We used a PACS-integrated LTT to track the treatment response of each lesion after first GK intervention to maximally seven diagnostic follow-up scans. We evaluated the efficiency of this tool by comparing the number of clicks necessary to complete this task with and without the tool and examined the prevalence of HeR in treatment.ResultsA cohort of eighty patients was selected and 494 lesions were measured and tracked longitudinally for a mean follow-up time of 374 days after first GK. Use of LTT significantly decreased number of necessary clicks. 81.7% of patients had HeR to treatment at the end of follow-up. The prevalence increased with increasing number of lesions.ConclusionsLesions in a single patient often differ in their response to treatment, highlighting the importance of individual lesion size assessments for further treatment planning. PACS-integrated lesion tracking enables efficient lesion surveillance workflow and specific and objective result reports to treating clinicians.
Dynamic confinement of SAPO-17 cages on the selectivity control of syngas conversion
Authors: Wang Haodi , Jiao Feng , Ding Yi , Liu Wenjuan , Xu Zhaochao , Pan Xiulian , Bao Xinhe 
Published: July 2022

Abstract
The OXZEO (oxide−zeolite) bifunctional catalyst concept has enabled selective syngas conversion to a series of value-added chemicals and fuels such as light olefins, aromatics and gasoline. Herein we report for the first time a dynamic confinement of SAPO-17 cages on the selectivity control of syngas conversion observed during an induction period. Structured illumination microscopy, intelligent gravimetric analysis, UV-Raman, X-ray diffraction, thermogravimetry and gas chromatography-mass spectrometer analysis indicate that this is attributed to the evolution of carbonaceous species as the reaction proceeds, which gradually reduces the effective space inside the cage. Consequently, the diffusion of molecules is hindered and the hindering is much more prominent for larger molecules such as C4+. As a result, the selectivity of ethylene is enhanced whereas that of C4+ is suppressed. Beyond the induction period, the product selectivity levels off. For instance, ethylene selectivity levels off at 44% and propylene selectivity at 31%, as well as CO conversion at 27%. The findings here bring a new fundamental understanding that will guide further development of selective catalysts for olefin synthesis based on the OXZEO concept.
Epidemiology and Factors Associated With Discharging Patients After Blood Culture Collection in the Emergency Department: A Case-Control Study in Japan
Authors: Miwa Toshiki , Takamatsu Akane , Honda Hitoshi 
Published: July 2022

Abstract

Background
Some patients receive the diagnosis of bloodstream infection (BSI) after discharge from the emergency room (ER). Because the safety of discharging patients after a blood culture collection is unknown, the present study aimed to investigate the prevalence, outcomes, and factors associated with BSI diagnosed after ER discharge.

Methods
This monocentric, case-control study compared patients who received a BSI diagnosis after ER discharge with those who were admitted for BSI. Factors associated with ER discharge after a blood culture collection were identified using multivariate logistic regression analysis.

Results
Between January 2014 and December 2020, 5.5% (142/2575) of patients with BSI visiting the ER were initially discharged. This occurred more commonly during the coronavirus disease 2019 (COVID-19) pandemic in 2020. On multivariate analysis, factors independently associated with the discharge of patients with BSI were the absence of hypotension (adjusted odds ratio [aOR], 15.71 [95% confidence interval {CI}, 3.45–71.63]), absence of altered mental status in the ER (aOR, 8.99 [95% CI, 3.49–23.14]), unknown origin at ER discharge (aOR, 4.60 [95% CI, 2.43–8.72]), and low C-reactive protein (aOR, 3.60 [95% CI, 2.19–5.93]). No difference in 28-day mortality was observed between the groups.

Conclusions
BSI is occasionally diagnosed after ER discharge. The prevalence of BSI diagnosed after ER discharge may have increased during the COVID-19 pandemic. Normal vital signs, unknown origin at ER discharge, and low C-reactive protein were important considerations leading to the discharge of these patients.
Sensitivity of the Elecsys Nucleocapsid Assay for the Detection of Preceding SARS-CoV-2 Infections
Authors: Grunau Brian , Tom Janessa , Asamoah-Boaheng Michael , O’Brien Sheila F , Drews Steven J , Sediqi Sadaf , Lavoie Pascal M , Barakauskas Vilte , Goldfarb David M 
Published: July 2022

Abstract
Nucleocapsid serological assay sensitivity to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections among vaccinees and for Omicron cases is unclear. In this prospective study, the Elecsys nucleocapsid assay was 89% sensitive in identifying SARS-CoV-2 infections 14–607 days pre–blood collection. Sensitivity was similar when comparing by vaccination status, and in Omicron (vs pre-Omicron) cases.
Thoraco-abdominal duplication cyst of the jejunum presenting as respiratory distress in a young infant—a case report
Authors: Harputluoğlu Nilgün , Çelik Tanju , Temir Günyüz , Hoşgör Münevver 
Published: July 2022

ABSTRACT
Thoraco-abdominal duplication cysts are rare congenital anomalies that can accompany vertebral and spinal cord pathologies and occur most frequently in the small intestines. Symptoms such as respiratory distress, dyspnea, tachypnea, cough, hemoptysis, cyanosis, vomiting and dysphagia may develop depending on the location. The cyst has several clinical and radiological dilemmas. We present a rare case of thoraco-abdominal duplication cyst in a 3-month-old male patient presenting with respiratory distress in the neonatal period. Thoraco-abdominal duplications require a high index of suspicion and meticulous clinical management. Thus, patients can be successfully managed without any unnecessary interventions, complications and loss of time.
Extensive aortic thrombosis and testicular infarction – a rare complication of biventricular cardiac thrombi
Authors: Ezad Saad M , Salmon Andrew , Cheema Hooria , Swallow Rosie 
Published: July 2022

ABSTRACT
Heart failure is a heterogenous syndrome which is increasing in prevalence, with a prognosis worse than many malignancies. Morbidity and mortality most commonly occur secondary to pump failure or ventricular arrhythmias; however, a more infrequently seen complication is the formation of mural thrombi. More commonly seen within the left ventricle, thrombi can embolize leading to stroke or end organ infarction. We present the case of a male who presented with decompensated heart failure. The presence of biventricular thrombi was found on echocardiography and subsequent cross-sectional imaging revealed these had embolized resulting in the rare complication of extensive abdominal aortic thrombosis with renal and testicular infarction. Biventricular thrombi are rare but high risk due to the potential for embolization as demonstrated in this case. Prompt recognition and management with anti-coagulation are essential, followed by treatment of the underlying pathology, which resulted in the formation of thrombi to prevent recurrence.
Emergency surgical decompression and long-term survival of glioblastoma presenting in coma in old age: case report and review of prognostic factors
Authors: McKinley Aidan , Lammy Simon , James Allan , Stan Alexandru , Barrett Chris 
Published: July 2022

ABSTRACT
We present a case demonstrating that older age does not exclude long-term survival with glioblastoma. This is a malignant neoplasm with a median life expectancy of 14 months in patients treated with radical intent. Survival is dependent on several independent and interacting prognostic factors of which advancing age is a negative factor. We present a septuagenarian with a 3.5-year survival following aggressive management. The potential to improve glioblastoma survival in an elderly population by examination of additional prognostic factors and identifying biomarkers warrants further research.