bluuebunny/crossref_metadata_2025_split huggingface dataset:
The assessment of mesenchymal stem cells characteristics in cultured amniotic fluid cells
Authors: SEZER Ozlem 
Published: June 2022

OBJECTİVES: To evaluate the morphological characteristics, immunophenotypic features, osteogenic, adipogenic, and chondrogenic differentiation capacities of in-vitro cultured amniotic cells during the culture process based on mesenchymal stem cell (MSC).
MATERİAL/METHOD: This study used waste cells remaining after fetal karyotype determination with second-trimester amniotic cell culture. Cultured cells were morphologically evaluated daily. Selective subculture (SSC) was produced by the scraping method. Immunophenotypic features of primary and subculture amniotic cells were evaluated based on specific surface antigens. Specific growth mediums were used to assess their osteogenic, adipogenic, and chondrogenic differentiation abilities. Differentiation was confirmed using specific staining.
RESULTS: Fifteen cases with 46,XY fetal cytogenetic analyses at 16+3-19+5 gestational weeks were included in the study. Amniotic fluid cells were evaluated morphologically in three classes as Amniotic fluid specific cell-type(AF), Fibroblastoid cell-type(FB1, FB2), and Epithelioid cell-type(E). The first cell adhesions occurred in the first 24-48 hours(36%) of cell culture, while the first colonies within three days(82%). SSC was produced in AF-SSC(n=4) and FB2-SSC(n=6) directions by mechanical scraping. Primary amniotic fluid cells and AF/FB-SSCs were positive for MSC immunophenotypic markers CD29,CD73,CD166,CD44,CD49e,CD90, while negative for CD34,CD45, and HLA-DR. Cells were shown to have osteogenic (Alizarin Red-S) and chondrogenic (Alcian Blue) differentiation potentials based on histochemical staining, while adipogenic (Oil Red-O) differentiation was not obtained.
CONCLUSION: We believe that preliminary protocols and experiences to obtain MSCs from amniotic fluid-derived cells produced under routine prenatal diagnosis cell culture conditions can enable many promising pre-clinical/clinical studies and be the pioneer of "stem cell therapy applications" in many "incurable" diseases in the clinic.
ANALISIS PENGGUNAAN KOSAKATA PADA AKUN INSTAGRAM RESMI UIN SAYYID ALI RAHMATULLAH TULUNGAGUNG
Authors: Rianto Krisna Dian 
Published: June 2022

Bahasa merupakan alat dan sarana dalam berkomunikasi dikehidupan sehari-hari. Kosakata adalah himpunan kata yang diketahui oleh seseorang atau entitas lain, atau merupakan bagian dari suatu bahasa tertentu. Sedangkan bahasa gaul adalah bahasa yang biasanya muncul karena sering digunakannya istilah-istilah baru oleh pengguna bahasa. Bahasa gaul termasuk salah satu variasi bahasa yang digunakan masyarakat terutama dari kalangan selebritis dan kalangan muda sebagai bahasa santai dalam komunikasi sehari-hari untuk menambah rasa keakraban dan keintiman di antara mereka. Tujuan penulis dalam artikel ini yaitu untuk menganalisis penggunaan kosakata pada akun media sosial Instagram UIN Sayyid Ali Rahmatullah Tulungagung.
MAKNA KONOTATIF DALAM ANTOLOGI PERJAMUAN KHONG GUAN KARYA JOKO PINURBO (KAJIAN SEMANTIK)
Authors: Cahyono Koko , Palupi Muncar Tyas , Kusumaningrum Rika Novita 
Published: June 2022

Tujuan dari penelitian ini, yaitu untuk mendeskripsikan analisis makna konotatif dalam antologi Perjamuan Khong Guan Karya Joko Pinurbo.Penelitian ini termasuk ke dalam jenis penelitian kualitatif deskriptif. Data yang digunakan dalam penelitian ini adalah penggalan baris kata, frasa, klausa atau kalimat dalam bait puisi kaleng keempat pada antologi Perjamuan Khong Guan karya Joko Pinurbo. Teknik pengumpulan data yang digunakan dalam penelitian ini adalah teknik baca dan teknik catat. Hasil penelitian ini diperoleh dari 22 puisi dalam kaleng keempat antologi Perjamuan Khong Guan. Kemudian dari 22 puisi tersebut, ditemukan 28 data makna konotatif yang terdiri dari 14 makna konotatif positif, 8 makna konotatif negatif, dan 6 makna konotatif netral. Dalam 28 data tersebut juga meliputi 47 makna konotatif yang dapat berupa kata, frasa, klausa atau kalimat yang bermakna konotatif.
METODE OBSERVASI MENINGKATKAN KOMPETENSI MENYUSUN TEKS EKSPOSISI DAN PUISI BEBAS PARA SISWA KELAS VIII SMP N 1 KASIHAN
Authors: Jayati Siti Sri 
Published: June 2022

Penelitian ini bertujuan meningkatkan keterampilan menyusun teks eksposisi dan puisi bebas para siswa Kelas VIII C SMP Negeri 1 Kasihan semester 1, tahun pelajaran 2021/2022 dengan metode observasi. Jenis penelitian adalah penelitian tindakan kelas dengan subjek penelitian 32 siswa. Prosedur penelitian dilakukan dengan tahap perencanaan, tindakan, observasi, dan refleksi. Penelitian dilaksanakan dua siklus. Teknik pengumpulan data dengan cara observasi, wawancara, dan analisis dokumen. Analisis dilakukan secara kualitatif dan kuantitatif. Hasil penelitian menunjukkan bahwa metode observasi dapat meningkatkan keaktifan para siswa dalam menyusun teks eksposisi dan puisi bebas. Pada Siklus 1 menyusun teks eksposisi, guru menerapkan tindakan pada siswa untuk mengobservasi potretan tanaman dan benda di lingkungan rumah terkait dengan pisang klutuk dan jeruk nipis yang di-share di Grup KBM Online. Pada siklus 2 menyusun teks puisi bebas, guru menerapkan metode observasi agar para siswa mengamati potretan benda atau tanaman di lingkungan rumah yang berbeda dengan siklus 1 yaitu benda-benda alat-alat makan dan tanaman-tanaman hias yang di-share di Grup KBM Online. Pada siklus 1 metode ini meningkatkan keterampilan menyusun teks eksposisi terkait dengan penyusunan kalimat/bahasa pada struktur tesis, rangkaian argumen, dan penegasan ulang, dengan rerata nilai 77, 34. Ketuntasan belajar pada Siklus 1 mencapai 78, 12 %. Pada Siklus 2 metode ini meningkatkan keterampilan menyusun teks puisi bebas dalam menyusun larik, diksi, majas, dan citraan dengan rerata nilai 79,66. Ketuntasan belajar pada siklus 2 mencapai 100 %.
Туристичні ресурси Кривого Рогу як основа розвитку екологічного туризму
Authors: Кельзікова Г.К. , Шипунова В.О. 
Published: June 2022

Традиційно екологічний туризм розуміється переважно як відвідування найбільш екологічно врівноважених, незабруднених, заповідних територій. Такій позиції сприяла потреба людини вирватись з міст, насичених технікою, урбанізованих і промислових ландшафтів. Об’єктами туризму в такому випадку є заповідні території - національні парки, заповідники, ботанічні сади, зоологічні парки, заказники, пам’ятки природи, дендрологічні парки тощо. Інша сторона екологічних процесів і станів територій - антропогенні порушення природи як потенційні альтернативні об’єкти спостережень, залишаються осторонь. Тому постала задача переосмислення традиційного підходу до визначення змісту екологічного туризму. Всі сучасні ландшафти в тій чи іншій мірі перетворені людиною. Ми вважаємо, що до змісту екологічного туризму слід відносити й подорожі до регіонів і об’єктів, які зазнали якісних змін з боку людини.
Використання інтерактивних технологій при викладанні біології та екології
Authors: Нікола А.А. 
Published: June 2022

З-поміж запропонованої сьогоденням великої кількості інноваційних технологій навчання, визначають переваги роботи в режимі інтерактиву, який найбільш повно забезпечує комфортні, безконфліктні умови розвитку студентів. Тому в наш час актуальною проблемою є використання інтерактивних вправ при викладанні біології та екології.
FEMALE MASCULINITY IN THE NOVEL THE SPY BY PAULO COELHO: A REVIEW ON THE MAIN CHARACTER
Authors: Rahayu Dewi , Widisanti S Ni Made , Yukesti Tetty 
Published: June 2022

ABSTRACTThis study aims at denoting the image of female masculinity through the study of the main character in the novel The Spy by Paulo Coelho. The main character, Mata Hari, works as a dancer and hetaira, but is accused of spying because of her profession. The method used in this research is descriptive qualitative with library research technique. The results of this study indicate that female masculinity described in the main character is considered hegemonic masculinity, that is, the kind of masculinity formed through behavior, personal experience, and cultural processes. This masculinity is denoted in the presence of the main character’s characteristics, such as self-nature, adventurous, free, selfish, reckless, active, and courageous.Keyword : Female masculinity, Hetaira, Masculinity-femininity.
THE RELATIONSHIP BETWEEN SYMBOL AND MEANING IN THE FILM ZOOTOPIA
Authors: Septiani Dwi Lestari , Rejeki Sari , Widisanti S Ni Made 
Published: June 2022

ABSTRACTThis research discusses the relationship between the symbol and meaning in Zootopia, a film by Jared Bush and Phil Johnston. The objectives of this research is to show the types of symbol in Zootopia and its meaning by identifying the types of symbol and its meaning based on the speaker’s utterance and the context of the utterance itself. The method used in this research is descriptive analysis with library research technique. The results of this research shows that there are 15 symbols in this movie. From 15 symbols mentioned, all the symbols in Zootopia are private symbol. The meaning of symbol are determined by its reference, which refers to the words and sentences, and also by supportive context which explains the reference. Symbols and meanings in this movie are closely related and become a unity that builds storylines and characters. The symbols in this movie are intentionally used to help in conveying the content and message of the movie. Keywords: Symbol, meaning, movie.
Gender-Based Violence Againsts The Female Main Character in Colleen Hoover’s It Ends With Us
Authors: Lestari Taria Ayu , Setyowati Agnes , Yukesti Tetty 
Published: June 2022

Abstract This research is a study of Colleen Hoover’s novel It Ends With Us. The purpose of this study is to reveal forms and impacts of gender-based violence and efforts of the female main character to overcome all the hardship. The main issue in this novel is gender-based violence againts the female main character named Lily Bloom. In this research, descriptive analysis method is used in studying the intrinsic theories, and extrinsic theories such as gender and feminism for supporting elements. The findings of this research points that Lily experiences several forms of gender-based violences such as child abuse, physical attacks, domestic violence, pornography violence, and an act of rape. Besides that, the physical and psychological impacts are also identified. Eventually, Lily can overcome her hardship by improving her financial condition, living independently, divorcing her abusive partner, and becoming a single parent. Both internal and external factors are in support of her measure to survive and live a better life. Keywords: feminism, gender, gender-based violence, women
SOCIAL DILEMMA OF THE MAIN CHARACTER IN THE NOVEL THE HEIR BY KIERA CASS
Authors: Nugroho Ryan Adi , Suharyati Henny , Natawiria Ade S 
Published: June 2022

In this thesis, the problem discussed focuses on the influence of the social dilemma of the main character in the novel The Heir by Kiera Cass. Eadlyn's dilemma was a problem she faced in her own family that forced her to complete The Selection and her dream of becoming a royal ruler. This research uses descriptive analyst method, based on the psychological approach of ID, ego, super ego and research techniques based on library research. The results of the research prove that dilemma can influence the decision of the main character that makes the main character sacrifice her dreams to her parents for the sake of the kingdom rather than her own desires. The dilemma of exogenous factors experienced by the main character in the novel The Heir by Kiera Cass has a greater role in influencing decisions taken by the main character and it also affects the social life of the main character in the form of conflict between the main character and her family and changes in her nature, from being stubborn to being caring and compassionate.Keywords: dilemma, conflict, main character
PRAGMATIC ANALYSIS OF TEACHER UTTERANCES IN A VIRTUAL CLASSROOM
Authors: Rejeki Sari 
Published: June 2022

This article is interested in studying the utterances used by a teacher in a virtual classroom. The study is conducted in Kindergarten 2 class, James Education Centre located in Bogor. The method applied is descriptive qualitative, using recordings and note taking as a technique to attain data. Looking into the illocutionary act, which is the meaning of utterances proposed by Searle, the data reveals that 69% of the teacher utterances are directives, 18% assertives and 12% expressives. The meaning of directive speech act is commiting interlocuters to do something. Assertives are used as statements and reports, while expressives as compliments and expressing gratitude. Keywords: utterance; speech act; illocutionaryact; directives.
Analisis Penggunaan Kanjou Hyougen Dalam Drama Mouichido Kimi Ni Propose
Authors: Febriani Gita , Sudjianto Sudjianto , Karyati Alo 
Published: June 2022

Abstrak Penelitian ini membahas tentang penggunaan kanjou hyougen yang terdapat dalam drama Jepang. Kanjou hyougen adalah ungkapan emosional. Ungkapan emosional disini dapat berupa ungkapan senang, sedih, takut, suka, marah, malu, dan sebagainya. Objek penelitian ini adalah kanjou hyougen yang terdapat dalam drama Mouichido Kimi Ni Propose. Pembahasannya lebih fokus kepada kanjou hyougen dalam drama tersebut, kategori beserta maknanya.Kata kunci: hyougen, kanjou hyougen
Analisis Penggunaan Wakamono Kotoba yang Populer di Kalangan Wanita Jepang Dalam Media Sosial Twitter
Authors: Sayekti Sapta Inderawan , Fitriana Rina , Karyati Alo 
Published: June 2022

Abstrak Penelitian ini membahas penggunaan dari wakamono kotoba yang populer di kalangan wanita Jepang. Fokus utama dari skripsi ini adalah jenis-jenis kelas kata, pembentukan kata, perubahan makna kata, dan fungsi dari masing-masing wakamono kotoba yang populer di kalangan wanita Jepang berdasarkan survei popularitas penggunaan wakamono kotoba yang dilakukan oleh situs Jikitourai.net. Teori yang digunakan adalah teori morfologi dan semantik di mana sebuah kata dianalisa makna dan pembentukannya. Kata Kunci : Wakamono Kotoba, Makna, Pembentukan kata
Analisis Penggunaan Bahasa Sopan dalam Serial Animasi Hyouka
Authors: Rachmadita Rachmadita , Fitriana Rina , Karyati Alo 
Published: June 2022

Abstrak Skripsi ini membahas tentang penggunaan ragam jenis bahasa sopan atau dalam bahasa Jepang disebut keigo, yang dianalisis berdasarkan dengan kondisi, situasi, usia, dan juga status sosial. Objek dalam penilitian ini adalah susunan pembentuk kalimat dan hal yang melatarbelakangi penggunaan keigo yang dihubungkan dengan keadaan sosial masyarakat oleh karakter Chitanda Eru. Keigo yang dituturkan oleh karakter Chitanda ini ada tiga jenis yaitu sonkeigo, kenjougo, dan juga teineigo. Dan hal yang melatarbelakangi karakter Chitanda tersebut selalu menggunakan keigo antara lain karena status kedudukan Chitanda yang memiliki latar belakang keluarga ternama dan cukup berpengaruh di kotanya, selain itu Chitanda juga merupakan seorang siswi berprestasi di sekolahnya, untuk menjaga kehormatan dan martabat keluarga, lalu Chitanda ingin menunjukan jati dirinya sebagai anak satu-satunya dari keluarga bangsawan, dan yang terakhir adalah rasa tanggung jawab terhadap keluarganya sangat besar. Karena di Jepang, dengan merendahkan diri dengan bahasa atau perilaku, tidak membuat pembicara akan dipandang rendah oleh lawan bicaranya, melainkan akan dipandang sebagai orang yang beretika dan sopan. Kata kunci: Keigo, Sonkeigo, Kenjougo, Teineigo, bahasa sopan, Chitanda Eru
ANALISIS PERTENTANGAN PANDANGAN ANTARA GENERASI X DENGAN GENERASI MILENIAL TENTANG BUDAYA POPULER (Studi Semiotika Roland Barthes Pada Film My Generation)
Authors: Ramadhanti Widia , Firdaus Dwi Rini Sovia , Trikusumaningtias Intan 
Published: June 2022

This research aims to identify conflicting views between generation x figures with millennials in My Generation film that shows the dynamics of millennial life in the era of technological development and popular culture development interpreted through adegans and dialogues in adegans certain. This study uses a qualitative method with Roland Barthes's semiotic analysis which has the concepts of denotation, connotation and myth. Analysis of the whole adegan, six adegans have been taken that represent the conflicting views between x generation with millennial generation in the film my generation. The six adegans are analyzed through denotation and connotation and a myth appears in several adegans. The results of this study can be concluded that the conflict of views between generation x (as parents), and millennial generation (as children) towards popular culture, occurs because of differences in lifestyle and differences in the way in responding to the cycle of cultural movement.
Multi-Objective Optimization Design of Ladle Refractory Lining Based on Genetic Algorithm
Authors: Sun Ying , Huang Peng , Cao Yongcheng , Jiang Guozhang , Yuan Zhongping , Bai Dongxu , Liu Xin 
Published: June 2022

Genetic algorithm is widely used in multi-objective mechanical structure optimization. In this paper, a genetic algorithm-based optimization method for ladle refractory lining structure is proposed. First, the parametric finite element model of the new ladle refractory lining is established by using ANSYS Workbench software. The refractory lining is mainly composed of insulating layer, permanent layer and working layer. Secondly, a mathematical model for multi-objective optimization is established to reveal the functional relationship between the maximum equivalent force on the ladle lining, the maximum temperature on the ladle shell, the total mass of the ladle and the structural parameters of the ladle refractory lining. Genetic algorithm translates the optimization process of ladle refractory lining into natural evolution and selection. The optimization results show that, compared with the unoptimized ladle refractory lining structure (insulation layer thickness of 0 mm, permanent layer thickness of 81 mm, and working layer thickness of 152 mm), the refractory lining with insulation layer thickness of 8.02 mm, permanent layer thickness of 76.20 mm, and working layer thickness of 148.61 mm has the best thermal insulation performance and longer service life within the variation of ladle refractory lining structure parameters. Finally, the results of the optimization are verified and analyzed in this paper. The study found that by optimizing the design of the ladle refractory lining, the maximum equivalent force on the ladle lining, the maximum temperature on the ladle shell and the ladle mass were reduced. The thermal insulation performance and the lightweight performance of the ladle are improved, which is very important for improving the service life of the ladle.
Design, Synthesis, and Quorum Quenching Potential of Novel Catechol–Zingerone Conjugate to Find an Elixir to Tackle Pseudomonas aeruginosa Through the Trojan Horse Strategy
Authors: Mangal Surabhi , Dua Tamanna , Chauhan Monika , Dhingra Neelima , Chhibber Sanjay , Singh Vasundhara , Harjai Kusum 
Published: June 2022

To address the issue of multidrug resistance in Pseudomonas aeruginosa, a novel catechol–zingerone conjugate (1) linked via a non-hydrolyzable 1,2,3-triazole linker was synthesized and subjected to biological evaluation based on the Trojan horse strategy. To enhance the efficacy, catechol, a xenosiderophore, utilized by P. aeruginosa for iron assimilation, and the dietary phytochemical zingerone, known for its anti-virulent activity against Pseudomonas aeruginosa, were exploited in the present study. Theoretical validation of conjugate (1) was conducted by in silico molecular docking analysis to determine the interaction with outer membrane transport receptor PirA and quorum sensing signal receptors. In addition, nine-fold binding affinity of Conjugate (1) toward PirA (5FP2) in comparison to its natural ligand catechol with D-score −1.13 Å authenticated the designed Trojan horse drug. Conjugate (1) showed stronger anti-virulent activity than zingerone; hence, it exhibited a promising anti-biofilm efficacy as assessed by crystal violet assay and visualized by FESEM toward P. aeruginosa. Encouraging results against P. aeruginosa in terms of quorum sensing regulated virulence factors, motility phenotypes, and biofilm formation with no cell cytotoxicity and could help open hitherto unexplored possibilities of establishing Trojan horse drugs as a successful approach against multidrug resistance in P. aeruginosa.
Clinical Insights to Complete and Incomplete Surgical Revascularization in Atrial Fibrillation and Multivessel Coronary Disease
Authors: Pasierski Michal , Staromłyński Jakub , Finke Janina , Litwinowicz Radoslaw , Filip Grzegorz , Kowalówka Adam , Wańha Wojciech , Kołodziejczak Michalina , Piekuś-Słomka Natalia , Łoś Andrzej , Stefaniak Sebastian , Wojakowski Wojciech , Jemielity Marek , Rogowski Jan , Deja Marek , Jagielak Dariusz , Bartus Krzysztof , Mariani Silvia , Li Tong , Matteucci Matteo , Ronco Daniele , Jiritano Federica , Fina Dario , Martucci Gennaro , Meani Paolo , Raffa Giuseppe Maria , Słomka Artur , Malvidni Pietro Giorgio , Lorusso Roberto , Zembala Michal , Suwalski Piotr , Kowalewski Mariusz 
Published: June 2022

ObjectivesAlthough endorsed by international guidelines, complete revascularization (CR) with Coronary Artery Bypass Grafting (CABG) remains underused. In higher-risk patients such as those with pre-operative atrial fibrillation (AF), the effects of CR are not well studied.MethodsWe analyzed patients’ data from the HEIST (HEart surgery In AF and Supraventricular Tachycardia) registry. Between 2012 and 2020 we identified 4770 patients with pre-operative AF and multivessel coronary artery disease who underwent isolated CABG. We divided the cohort according to the completeness of the revascularization and used propensity score matching (PSM) to minimize differences between baseline characteristics. The primary endpoint was all-cause mortality.ResultsMedian follow-up was 4.7 years [interquartile range (IQR) 2.3–6.9]. PSM resulted in 1,009 pairs of complete and incomplete revascularization. Number of distal anastomoses varied, accounting for 3.0 + –0.6 vs. 1.7 + –0.6, respectively. Although early (&lt; 24 h) and 30-day post-operative mortalities were not statistically different between non-CR and CR patients [Odds Ratio (OR) and 95% Confidence Intervals (CIs): 1.34 (0.46–3.86); P = 0.593, Hazard Ratio (HR) and 95% CIs: 0.88 (0.59–1.32); P = 0.542, respectively] the long term mortality was nearly 20% lower in the CR cohort [HR (95% CIs) 0.83 (0.71–0.96); P = 0.011]. This benefit was sustained throughout subgroup analyses, yet most accentuated in low-risk patients (younger i.e., &lt; 70 year old, with a EuroSCORE II &lt; 2%, non-diabetic) and when off-pump CABG was performed.ConclusionComplete revascularization in patients with pre-operative AF is safe and associated with improved survival. Particular survival benefit with CR was observed in low-risk patients undergoing off-pump CABG.