bluuebunny/crossref_metadata_2025_split huggingface dataset:
The underlying mechanism of Guillain-Barré syndrome in a young patient suffered from Japanese encephalitis virus infection: a case report
Authors: Liu Sheng , Wang Jinyong , Yang Jun , Wen Ying 
Published: September 2022

Abstract
Background
The presentation of Guillain–Barré syndrome (GBS) caused by Japanese encephalitis virus (JEV) is uncommon, although clusters of GBS cases were observed in China in 2018. The underlying mechanism is unclear, particularly in individuals vaccinated against Japanese encephalitis in childhood.

Case presentation
We report a patient with acute flaccid paralysis involving four extremities and respiratory muscles, while magnetic resonance imaging of the brain and spine were standard. Electrophysiological examination displayed slowed motor nerve conduction speed and reduced evoked velocity amplitude. GBS was finally considered which was related to JEV infection verified by positive anti-JEV immunoglobulin M antibody and positive immunoglobulin G antibody in the serum. Unfortunately, the patient refused intravenous immunoglobulin and declined the use of mechanical ventilation again. He voluntarily withdrew from the hospital and died on the 36th day after the onset of illness. We also performed a review of previously reported related cases and discussed the underlying mechanism.

Conclusion
JEV infection-associated GBS is unusual. We should pay attention to the atypical manifestations of JEV infection and explore possible pathogenesis in particular individuals.
Eight-year operation status and data analysis of the first human milk bank in East China
Authors: Xiaoshan Hu , Xue Chu , Jun Zhang , Feng Liu , Xiaohui Chen , Zhangbin Yu , Shuping Han 
Published: September 2022

Abstract
Background
Human milk banks (HMBs) are essential facilities for the selection, collection, testing, transportation,storage, and distribution of DHM for special medical needs. The aim of this analysis was to analyze the operation status and data over the last 8 years of operation of the first human milk bank (HMB) in East China.

Methods
Data related to the costs, donors, donation, pasteurization, and recipients were extracted from the web-based electronic monitoring system of the HMB for the period August 1, 2013 to July 31, 2021.

Results
Over the 8 years of operation, 1,555 qualified donors donated 7,396.5L of qualified milk at a cost of ¥1.94 million($306,051), with the average cost per liter of donor human milk being ¥262.3($41.4). The donors were between 25 and 30 years of age, and the majority (80.1%) were primipara. All the donated milk was pasteurized and subjected to bacteriological tests before and after pasteurization: 95.4% passed the pre-pasteurization tests, and 96.3% passed the post-pasteurization tests. A total of 9,207 newborns received 5,775.2L of pasteurized donor milk. The main reason for the prescription of donor human milk was preterm birth. As a result of continuous quality improvements, January 2016 witnessed a significant increase in the volume of qualified DHM and the number of qualified donors. However, in 2020, as a result of the restrictions related to the COVID-19 pandemic, the volume of qualified DHM and the number of qualified donors decreased.

Conclusions
Over its 8 years of operation, our HMB has made steady quality improvements in its screening and information processes. Continuous quality improvement is on ongoing need, along with recruiting more qualified donors and collecting donor human milk for vulnerable newborns.
A gene expression profile for the lower osteogenic potent of bone-derived MSCs from osteoporosis with T2DM and the potential mechanism
Authors: Xia Sheng-li , Ma Zi-yuan , Wang Bin , Gao Feng , Guo Sheng-yang , Chen Xu-han 
Published: September 2022

Abstract
Background
Osteoporosis (OP) patients complicated with type II diabetes mellitus (T2DM) has a higher fracture risk than the non-diabetic patients, and mesenchymal stem cells (MSCs) from T2DM patients also show a weaker osteogenic potent. The present study aimed to provide a gene expression profile in MSCs from diabetic OP and investigated the potential mechanism.

Methods
The bone-derived MSC (BMSC) was isolated from OP patients complicated with or without T2DM (CON-BMSC, T2DM-BMSC). Osteogenic differentiation was evaluated by qPCR analysis of the expression levels of osteogenic markers, ALP activity and mineralization level. The differentially expressed genes (DEGs) in T2DM-BMSC was identified by RNA-sequence, and the biological roles of DEGs was annotated by bioinformatics analyses. The role of silencing the transcription factor (TF), Forkhead box Q1 (FOXQ1), on the osteogenic differentiation of BMSC was also investigated.

Results
T2DM-BMSC showed a significantly reduced osteogenic potent compare to the CON-BMSC. A total of 448 DEGs was screened in T2DM-BMSC, and bioinformatics analyses showed that many TFs and the target genes were enriched in various OP- and diabetes-related biological processes and pathways. FOXQ1 had the highest verified fold change (abs) among the top 8 TFs, and silence of FOXQ1 inhibited the osteogenic differentiation of CON-BMSC.

Conclusions
Our study provided a comprehensive gene expression profile of BMSC in diabetic OP, and found that downregulated FOXQ1 was responsible for the reduced osteogenic potent of T2DM-BSMC. This is of great importance for the special mechanism researches and the treatment of diabetic OP.
Decreased renal perfusion during acute kidney injury in critical COVID-19 assessed by magnetic resonance imaging: a prospective case control study
Authors: Luther Tomas , Eckerbom Per , Cox Eleanor , Lipcsey Miklos , Bülow Sara , Hultström Michael , Torrente Francisco Martinez , Weis Jan , Palm Fredrik , Francis Susan , Frithiof Robert , Liss Per 
Published: September 2022

Abstract
Background
Renal hypoperfusion has been suggested to contribute to the development of acute kidney injury (AKI) in critical COVID-19. However, limited data exist to support this. We aim to investigate the differences in renal perfusion, oxygenation and water diffusion using multiparametric magnetic resonance imaging in critically ill COVID-19 patients with and without AKI.

Methods
A prospective case–control study where patients without prior kidney disease treated in intensive care for respiratory failure due to COVID-19 were examined. Kidney Disease: Improving Global Outcomes Creatinine criteria were used for group allocation. Main comparisons were tested using Mann–Whitney U test.

Results
Nineteen patients were examined, ten with AKI and nine without AKI. Patients with AKI were examined in median 1 [0–2] day after criteria fulfillment. Age and baseline Plasma-Creatinine were similar in both groups. Total renal blood flow was lower in patients with AKI compared with patients without (median 645 quartile range [423–753] vs. 859 [746–920] ml/min, p = 0.037). Regional perfusion was reduced in both cortex (76 [51–112] vs. 146 [123–169] ml/100 g/min, p = 0.015) and medulla (28 [18–47] vs. 47 [38–73] ml/100 g/min, p = 0.03). Renal venous saturation was similar in both groups (72% [64–75] vs. 72% [63–84], ns.), as was regional oxygenation (R2*) in cortex (17 [16–19] vs. 17 [16–18] 1/s, ns.) and medulla (29 [24–39] vs. 27 [23–29] 1/s, ns.).

Conclusions
In critically ill COVID-19 patients with AKI, the total, cortical and medullary renal blood flows were reduced compared with similar patients without AKI, whereas no differences in renal oxygenation were demonstrable in this setting.
Trial registration ClinicalTrials ID: NCT02765191, registered May 6 2014 and updated May 7 2020.

Graphic Abstract
Decreased renal cortical perfusion, independent of changes in renal blood flow and sublingual microcirculatory impairment, is associated with the severity of acute kidney injury in patients with septic shock
Authors: Watchorn James , Huang Dean , Bramham Kate , Hutchings Sam 
Published: September 2022

Abstract
Background
Reduced renal perfusion has been implicated in the development of septic AKI. However, the relative contributions of macro- and microcirculatory blood flow and the extent to which impaired perfusion is an intrinsic renal phenomenon or part of a wider systemic shock state remains unclear.

Methods
Single-centre prospective longitudinal observational study was carried out. Assessments were made at Day 0, 1, 2 and 4 after ICU admission of renal cortical perfusion in 50 patients with septic shock and ten healthy volunteers using contrast-enhanced ultrasound (CEUS). Contemporaneous measurements were made using transthoracic echocardiography of cardiac output. Renal artery blood flow was calculated using velocity time integral and vessel diameter. Assessment of the sublingual microcirculation was made using handheld video microscopy. Patients were classified based on the degree of AKI: severe = KDIGO 3 v non-severe = KDIGO 0–2.

Results
At study enrolment, patients with severe AKI (37/50) had prolonged CEUS mean transit time (mTT) (10.2 vs. 5.5 s, p < 0.05), and reduced wash-in rate (WiR) (409 vs. 1203 au, p < 0.05) and perfusion index (PI) (485 vs. 1758 au, p < 0.05); differences persisted throughout the entire study. Conversely, there were no differences in either cardiac index, renal blood flow or renal resistive index. Sublingual microcirculatory variables were not significantly different between groups at study enrolment or at any subsequent time point. Although lactate was higher in the severe AKI group at study enrolment, these differences did not persist, and there were no differences in either ScvO2 or ScvCO2-SaCO2 between groups. Patients with severe AKI received higher doses of noradrenaline (0.34 vs. 0.21mcg/kg/min, p < 0.05). Linear regression analysis showed no correlation between mTT and cardiac index (R-0.18) or microcirculatory flow index (R-0.16).

Conclusion
Renal cortical hypoperfusion is a persistent feature in critically ill septic patients who develop AKI and does not appear to be caused by reductions in macrovascular renal blood flow or cardiac output. Cortical hypoperfusion appears not be associated with changes in the sublingual microcirculation, raising the possibility of a specific renal pathogenesis that may be amenable to therapeutic intervention.
Trial Registration Clinical Trials.gov NCT03713307, 19 Oct 2018.
MIRTH: Metabolite Imputation via Rank-Transformation and Harmonization
Authors: Freeman Benjamin A. , Jaro Sophie , Park Tricia , Keene Sam , Tansey Wesley , Reznik Ed 
Published: September 2022

AbstractOut of the thousands of metabolites in a given specimen, most metabolomics experiments measure only hundreds, with poor overlap across experimental platforms. Here, we describe Metabolite Imputation via Rank-Transformation and Harmonization (MIRTH), a method to impute unmeasured metabolite abundances by jointly modeling metabolite covariation across datasets which have heterogeneous coverage of metabolite features. MIRTH successfully recovers masked metabolite abundances both within single datasets and across multiple, independently-profiled datasets. MIRTH demonstrates that latent information about otherwise unmeasured metabolites is embedded within existing metabolomics data, and can be used to generate novel hypotheses and simplify existing metabolomic workflows.
Prediction of evolutionary constraint by genomic annotations improves functional prioritization of genomic variants in maize
Authors: Ramstein Guillaume P. , Buckler Edward S. 
Published: September 2022

AbstractBackgroundCrop improvement through cross-population genomic prediction and genome editing requires identification of causal variants at high resolution, within fewer than hundreds of base pairs. Most genetic mapping studies have generally lacked such resolution. In contrast, evolutionary approaches can detect genetic effects at high resolution, but they are limited by shifting selection, missing data, and low depth of multiple-sequence alignments. Here we use genomic annotations to accurately predict nucleotide conservation across angiosperms, as a proxy for fitness effect of mutations.ResultsUsing only sequence analysis, we annotate nonsynonymous mutations in 25,824 maize gene models, with information from bioinformatics and deep learning. Our predictions are validated by experimental information: within-species conservation, chromatin accessibility, and gene expression. According to gene ontology and pathway enrichment analyses, predicted nucleotide conservation points to genes in central carbon metabolism. Importantly, it improves genomic prediction for fitness-related traits such as grain yield, in elite maize panels, by stringent prioritization of fewer than 1% of single-site variants.ConclusionsOur results suggest that predicting nucleotide conservation across angiosperms may effectively prioritize sites most likely to impact fitness-related traits in crops, without being limited by shifting selection, missing data, and low depth of multiple-sequence alignments. Our approach—Prediction of mutation Impact by Calibrated Nucleotide Conservation (PICNC)—could be useful to select polymorphisms for accurate genomic prediction, and candidate mutations for efficient base editing. The trained PICNC models and predicted nucleotide conservation at protein-coding SNPs in maize are publicly available in CyVerse (https://doi.org/10.25739/hybz-2957).
Yogurt and molasses can alter microbial-digestive and nutritional characteristics of pomegranate leaves silage
Authors: Kazemi Mohsen , Valizadeh Reza , Ibrahimi Khoram Abadi Elias 
Published: September 2022

AbstractFewer studies in recent years have been conducted on the nutritional potential and fermentation quality of silage prepared from pomegranate leaves (PL). So, we investigated the nutritional-fermentation quality of PL before and after ensiling with or without yogurt containing mainly lactic acid-producing bacteria (Lactobacillus bulgaricusandStreptococcus thermophiles) and molasses (at two levels of 2 and 4% of dry matter) in the polyethylene microsilos for 60 days. A range of dry matter (29.1–39.1%), crude protein (3.85–4.83%), ash (5.33–8.60%), and non-fiber carbohydrates (53.2%–58.6%) contents were observed among the treatments. A significant increase in calcium, potassium, magnesium, manganese, iron, and zinc was observed in PL after ensiling compared to before ensiling (p < 0.05). The PL ensiled with 4% yogurt exhibited the highest ammonia nitrogen, lactic and acetic acids, but the lowest butyric acid among the ensiled PL (p < 0.05). The ensiling of PL without additive (control) significantly decreased potential gas production, dry matter digestibility, organic matter digestibility, total volatile fatty acids, metabolizable energy, net energy for lactation, base-buffering capacity, titratable alkalinity, and acid–base buffering capacity compared to before ensiling (p < 0.05). According to the present results, the nutritional value of PL before ensiling was higher than after ensiling. The addition of yogurt and molasses to PL at the ensiling process especially at 4% of dry matter, improved the fermentation and nutritional characteristics. In general, the addition of yogurt or molasses as two cheap and available additives is recommended to improve the digestive-fermentation parameters of PL in silo and ruminal environments.
Acupuncture for pain control after degenerative lumbar spine surgery
Authors: Chen Bo-An , Deng Wen-Chun , Chen Mao-Yu 
Published: September 2022

AbstractBackgroundWound pain after surgery for lumbar spine disease may interfere with patients’ recovery. Acupuncture is commonly used for pain management, but its efficacy for postoperative pain control is unclear. This study aimed to evaluate the effectiveness of acupuncture for adjuvant pain control after surgery for degenerative lumbar spine disease.MethodsWe retrospectively reviewed the records of consecutive patients who received surgery for degenerative lumbar spine disease at our institution from 2013 to 2014. Surgical procedures included open laminectomy, discectomy, and trans-pedicle screw instrumentation with posterior-lateral fusion. Patients were grouped by pain control methods, including routine analgesia, patient-controlled analgesia (PCA), and acupuncture. The routine analgesia group received oral acetaminophen/non-steroidal anti-inflammatory drugs with meperidine as needed for immediate pain control. The PCA group received a basal dose of morphine and subsequent user-demand doses. The acupuncture group received acupuncture every other day after surgery.ResultsNinety-six patients were included, of whom 37 received acupuncture, 27 received PCA, and 32 received routine analgesics for pain control. Visual analog scale (VAS) pain scores in all 3 groups decreased significantly, and to the same degree, from the first postoperative day to the second day. No significant differences were found in VAS scores over the next 6 postoperative days; however, the scores of patients treated with PCA were slightly but still significantly higher (p = 0.026) on postoperative day 4 than scores of patients treated with acupuncture and traditional analgesia, a difference likely due to PCA being discontinued on postoperative day 3. No major complications were noted in the acupuncture group, but 2 patients dropped out because of fear of needle insertion.ConclusionsAcupuncture may be as effective as traditional analgesia and PCA for adjuvant pain control after surgery for degenerative lumbar spine disease.
The effect of prior exposure on the lightfastness of early synthetic dyes on textiles
Authors: Hagan Eric , Poulin Jennifer 
Published: September 2022

Abstract
Many studies have investigated light-induced damage to colourants in heritage collections using prepared samples of materials such as artist paints and dyed textiles. The body of research focuses primarily on the response of virgin materials, where colour change is assessed with respect to the original colour as light dose increases. From a practical perspective, most objects in museum collections have accumulated a significant light dose from illumination before acquisition, and subsequent years of exhibit lighting. When considering the risk of further degradation, it is often stated by heritage professionals that fugitive colourants with past light exposure are no longer as sensitive due to the slowing rate of visual damage. This is evident by studying ‘fading curves’, where the rate of colour change typically diminishes with increasing light dose. It remains unclear, however, to what degree the lightfastness of remaining colour changes with ongoing exposure. To address the issue, the light sensitivity of residual colour was investigated as a function of prior dose using a published dataset of colour measurements from textiles dyed with early synthetic colourants in the period of 1874–1905. The CIELAB colour values (D65/2°) for each material and dose increment were used to determine the future dose that causes a just-noticeable difference (JND), ΔE
00 = 1.7, starting at different amounts of past exposure. This involved resetting the reference CIELAB values to that of the residual colour after each prior dose. The analysis provides an extension of our earlier work, where only the initial lightfastness was reported. A summary of the findings illustrates the shifting distribution of lightfastness, toward higher Blue Wool (BW) ratings with increasing exposure. As the dose progresses, dyes that start at BW1 sensitivity progress to BW2, then BW3 and so on. The findings from this work may assist with boundary approximations of object sensitivity when prior light exposure is known, or a reasonable estimate is available. An analysis of experimental data is summarised as a tool for this type of decision-making process.
Research on mechanical characteristics and energy dissipation of traditional ramming technology
Authors: Pei Qiangqiang , Liu Xiaoying , Hou Zhichun , Zhao Guojing , Zhang Bo , Guo Qinglin 
Published: September 2022

Abstract
The ramming technique is a traditional method used to improve the compactness of soil with impact and vibration energy. Based on the measured data of ramming force transmission and dissipation, this study reveals the mechanism of energy transfer and dissipation of single-layer ramming as well as the law of variation with ramming quality, laying thickness, and ramming times. It also establishes the ramming model and empirical formula of ramming quality, laying thickness, and ramming times, introduces the ramming layer changes associated with the constitutive relationship (elastic to elastoplastic parts) as a function of ramming times, and determines the influence depth and horizontal range based on real-time data monitoring. It was found that as the mass of the rammer increases, the range of influence of the impact stress inside the earth also increases proportionally, and this effect is more pronounced vertically. However, the dissipation rate of energy in horizontal direction is much greater than in the vertical direction. The traditional stacked ramming technique of “chong hai wo, hang yin ding” can effectively eliminate the defects of horizontal reversal upwelling impact and unevenness of the ramming layer during the ramming process. The research results provide a theoretical basis for evaluating the quality of the traditional ramming technique.
Controlling skin microbiome as a new bacteriotherapy for inflammatory skin diseases
Authors: Ito Yoshihiro , Amagai Masayuki 
Published: September 2022

AbstractThe skin serves as the interface between the human body and the environment and interacts with the microbial community. The skin microbiota consists of microorganisms, such as bacteria, fungi, mites, and viruses, and they fluctuate depending on the microenvironment defined by anatomical location and physiological function. The balance of interactions between the host and microbiota plays a pivotal role in the orchestration of skin homeostasis; however, the disturbance of the balance due to an alteration in the microbial communities, namely, dysbiosis, leads to various skin disorders. Recent developments in sequencing technology have provided new insights into the structure and function of skin microbial communities. Based on high-throughput sequencing analysis, a growing body of evidence indicates that a new treatment using live bacteria, termed bacteriotherapy, is a feasible therapeutic option for cutaneous diseases caused by dysbiosis. In particular, the administration of specific bacterial strains has been investigated as an exclusionary treatment strategy against pathogens associated with chronic skin disorders, whereas the safety, efficacy, and sustainability of this therapeutic approach using isolated live bacteria need to be further explored. In this review, we summarize our current understanding of the skin microbiota, as well as therapeutic strategies using characterized strains of live bacteria for skin inflammatory diseases. The ecosystem formed by interactions between the host and skin microbial consortium is still largely unexplored; however, advances in our understanding of the function of the skin microbiota at the strain level will lead to the development of new therapeutic methods.