bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide
Authors: Boncukcu Nilbeste , Akgul Busra , Akmayan Ilkgul , Berber Hale , Abamor Emrah Sefik , Ozbek Tulin , Derman Serap 
Published: November 2022

AbstractWound healing is a process getting affected by internal and external factors and might be interrupted by infections. To overcome infections during wound healing, novel antibacterial agents such as antimicrobial peptides have gained popularity because of the rising antibiotic resistance. Therefore, in this study, a three‐dimensional polymeric scaffold was designed for the controlled release of HF‐18 peptide, with the contribution of hyaluronic acid, chondroitin sulfate, and chitosan polymers with the crosslinker genipin. The obtained scaffold structure (OPT) was found to have interconnected pores, was pH‐responsive and swelled more in acidic conditions (5446.5% at pH: 5.0). It was observed that HF‐18‐loaded OPT (P‐OPT) was able to release HF‐18 peptide both in acidic and neutral conditions in a controlled release manner. This study also demonstrated that both OPT and P‐OPT were biocompatible and promoted L929 cell attachment and migration. Antimicrobial activity assessments demonstrated that P‐OPT was effectively bactericidal on Staphylococcus aureus and methicillin‐resistant S. aureus. Moreover, OPT produced a synergistic effect on the antimicrobial activity of HF‐18 peptide, as P‐OPT showed activity below the reported MIC value. As a result, OPT is considered a promising scaffold as a carrier for HF‐18 for wound healing.
Chemical Characterization, Antioxidant, Antimicrobial, Cytotoxicity and in Silico Studies of Hexane Extract and Essential Oils from Citrus limon Leaves
Authors: Riaz Muhammad , Qadir Rahman , Tahir Akhtar Muhammad , Misbah ur Rehman Muhammad , Anwar Farooq , Eman Rida , Fayyaz ur Rehman Muhammad , Safwan Akram Muhammad 
Published: November 2022

AbstractThe present study investigates the chemical composition, antioxidant and antimicrobial bioactivities of essential oil and hexane extract from Citrus limon leaves. The isolation of essential oil was carried out using the Clevenger apparatus. The percentage yield of essential oil and hexane extract from Citrus limon leaves was 0.59 and 0.50 %, respectively. 2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH) scavenging assay highlighted that Citrus limon leaves essential oil (CLEO) and hexane extract exhibited the significant antioxidant potential of 69.64 and 67.55 %, respectively, compared to the BHT standard. Similarly, a significant inhibition in linoleic acid peroxidation was recorded in both CLEO (81.93 %) and hexane extract (50.34 %). Characterization of chemical constituents in CLEO and extract was executed using GC/MS, where Limonene was detected as a major compound in CLEO (60.52 %) and hexane extract (73.62 %). The haemolytic activity ranged from 2.46 to 5.75 % revealing negligible cytotoxicity of CLEO and hexane extract. In silico studies agree with the in vitro antimicrobial studies, where vinimalol, taraxasterol, and moretenol present in CLEO showed strong interactions/inhibition against dihydroorotase and DNA gyrase from E. coli, and the tyrosyl‐tRNA synthetase and DNA gyrase from S. aureus. Based on the current data, it may be concluded that both CLEO and hexane extract possessed significant bioactivities, such as antimicrobial and antioxidant activity, with minimal cytotoxicity.
In Vitro Inhibitory Effects of Maqian Essential Oil against Ectopic Endometrial Stromal Cells and LPS‐Induced Endometrial Epithelial Cells
Authors: Li Li‐ping , Luo Yong , Huang Cheng , Wang Xin‐rong , Huang Ting‐ting , Zou Yong‐yi , Huang Shu‐hui , Liu Yan‐qiu , Yang Bi‐cheng 
Published: November 2022

AbstractPrevious studies revealed that MQEO (Maqian fruits essential oil), which is extracted from the fruit of Maqian (Zanthoxylum myriacanthum var. Pubescens), had a good anti‐inflammatory effect, but the effect on endometriosis in vitro remains unknown. In the present study, the inhibitory effects of MQEO against the EESCs (ectopic endometrial stromal cells) were investigated. Cells were treated with a concentration gradient (from 0.025 % to 0.15 %) of MQEO for 24 h and cell viability was detected by CCK‐8. In addition, apoptotic rates were investigated using flow cytometry. The effect of MQEO on cell migration was determined by wound‐healing and transwell assay. The expression of apoptosis‐associated and cell adhesion‐related proteins was assessed by western blotting. The transcriptional levels of IL‐1, IL‐6 and TNF‐α were determined by Real‐time qPCR. RNA‐seq was used to identify the DEGs (differentially expressed genes) in MQEO‐pretreated EESCs. We found that the MQEO condition dosage‐dependently reduced the cell viability of EESCs. Based on flow cytometry results, the number of apoptotic cells increased significantly with dosage. The wound‐healing and transwell results showed that MQEO group exhibited a significantly decreased cell motility and migration ability in comparison with the normal group. Western blotting results showed that MQEO down‐regulated the expression of Bcl‐2, ICAM‐1 (intercellular adhesion molecule 1) and CD44, but up‐regulated the cleaved caspase‐3 expression in EESCs. What's more, MQEO also inhibited the LPS‐induced inflammation in human EECs (endometrial epithelial cells). RNA‐seq revealed that 221 DEGs were up‐regulated genes and 284 DEGs were down‐regulated in MQEO‐pretreated EESCs. Our data uncovered the beneficial effects of MQEO in endometriosis and provided new insights into the mechanism of the effect of MQEO on EESCs, suggesting MQEO could be a promising new therapeutic agent for endometriosis.
Ozone therapy restores immune dysfunction in refractory idiopathic granulomatous mastitis as a novel potential therapeutic approach
Authors: Cabioglu Neslihan , Cetin Aktas Esin , Emiroglu Selman , Tukenmez Mustafa , Ozkurt Enver , Muslumanoglu Mahmut , Igci Abdullah , Ozmen Vahit , Deniz Gunnur , Dinccag Ahmet S. , Guven Yusuf I. 
Published: November 2022

AbstractImmunological dysfunction has been suggested to play a major role in the pathogenesis of idiopathic granulomatous mastitis (IGM). We recently showed that ozone therapy was effective in patients with steroid‐resistant IGM. This study assessed alterations in intracellular cytokine expression patterns in different T‐lymphocyte subsets after ozone therapy in refractory IGM. Peripheral blood T lymphocyte subsets (CD8+, CD4+, CD4+CD25+CD127−) were analyzed via flow‐cytometry for intracellular cytokine expressions IFN‐γ, TNF‐α, IL‐10, and TGF‐β before and after completion of 4‐month systemic ozone therapy. Ozone therapy significantly increased the CD4+IFN‐γ+ (p = 0.032), CD4+TNF‐α+ (p = 0.028), and the CD8+TNF‐α+ (p = 0.012) T cells. In contrast, significant decreases in CD4+ IL‐10+ (p = 0.047) and CD8+IL‐10+ T cells (p = 0.022) and CD4+CD25+CD127−//low Treg cells secreting TGF‐β (p = 0.005) were found after ozone therapy. When patients were analyzed according to the response to ozone therapy, patients with a complete remission were more likely to have increased CD3−CD16+CD56+ natural killer cells (p = 0.0027) and decreased CD19+ B lymphocytes (p = 0.046) following ozone therapy. Our results suggest that ozone therapy stimulated a T‐helper‐1 response associated with IFN‐γ production and downregulation of TGF‐β expression in CD4+CD25+CD127− Treg cells. These alterations in the immune system following ozone therapy can improve wound healing and restore immune dysfunction in patients with refractory IGM.
Membranous septum length may not be the answer
Authors: Ueyama Hiroki , Block Peter C. 
Published: November 2022

Key Points

Two different methods for measurement of membranous septum length have weak correlation and poor agreement.
Both approaches have significant interobserver variability in membranous septum length measurements.
Membranous septum length alone or in combination with implantation depth appears to provide no incremental value in predicting new pacemaker implantation after TAVR over implantation depth alone.
Clinical and echocardiographic outcomes with new‐onset left bundle branch block after SAPIEN‐3 transcatheter aortic valve replacement
Authors: Sammour Yasser M. , Lak Hassan , Chahine Johnny , Abushouk Abdelrahman , Chawla Sanchit , Kadri Amer , Alkhalaileh Feras , Kumar Sachin , Ghimire Bindesh , Svensson Lars , Popovic Zoran , Tarakji Khaldoun , Wazni Oussama , Reed Grant W. , Yun James , Puri Rishi , Krishnaswamy Amar , Kapadia Samir R. 
Published: November 2022

AbstractBackgroundNew‐onset left bundle branch block (LBBB) can develop after transcatheter aortic valve replacement (TAVR) resulting in worse outcomes.AimsDescribe clinical and echocardiographic outcomes with new‐onset LBBB after TAVR.MethodsWe included consecutive patients who underwent transfemoral‐TAVR with SAPIEN‐3 (S3) valve between April 2015 and December 2018. Exclusion criteria included pre‐existing LBBB, right BBB, left anterior hemiblock, left posterior hemiblock, wide QRS ≥ 120ms, prior permanent pacemaker (PPM), and nontransfemoral access.ResultsAmong 612 patients, 11.4% developed new‐onset LBBB upon discharge. The length of stay was longer with new‐onset LBBB compared with no LBBB [3 (2–5) days versus 2 (1–3) days; p < 0.001]. New‐onset LBBB was associated with higher rates of 30‐day PPM requirement (18.6% vs. 5.4%; p < 0.001) and 1‐year heart failure hospitalizations (10.7% vs. 4.4%; p = 0.033). There was no difference in 3‐year mortality between both groups (30.9% vs. 30.6%; p = 0.829). Further, new‐onset LBBB was associated with lower left ventricular ejection fraction (LVEF) at both 30 days (55.9 ± 11.4% vs. 59.3 ± 9%; p = 0.026) and 1 year (55 ± 12% vs. 60.1 ± 8.9%; p = 0.002). These changes were still present when we stratified patients according to baseline LVEF (≥50% or <50%). New‐onset LBBBs were associated with a higher 1‐year LV end‐diastolic volume index (51.4 ± 18.6 vs. 46.4 ± 15.1 ml/m2; p = 0.036), and LV end‐systolic volume index (23.2 ± 14.1 vs. 18.9 ± 9.7 ml/m2; p = 0.009). Compared with resolved new‐onset LBBB, persistent new‐onset LBBB was associated with worse LVEF and higher PPM at 1 year.ConclusionsNew‐onset LBBB after S3 TAVR was associated with higher PPM requirement, worse LVEF, higher LV volumes, and increased heart failure hospitalizations, with no difference in mortality.
Hypertrophic cardiomyopathy secondary to tacrolimus therapy in a kidney transplant patient: A case report and focused review of the literature
Authors: Hernández Silva Guillermo , Puerto Chaparro Ricardo Giovanni , Martínez Melo Javier Álvaro , Porras Bueno Cristian Orlando , Martínez Rodríguez Javier Eduardo , González Trillos Sharon Julieth 
Published: November 2022

AbstractCalcineurin inhibitors have become a pillar of immunosuppressive treatment in solid organ transplant recipients. Several case reports have shown hypertrophic and dilated cardiomyopathy as an adverse effect to tacrolimus therapy. We present the case of a kidney transplant recipient woman who developed hypertrophic cardiomyopathy due to tacrolimus therapy.
Ectopic abdominal pregnancy on the ascending colon
Authors: Suleiman Riham , Adwan Dema , Safadi Mhd Firas 
Published: November 2022

AbstractA 25‐year‐old woman presented to the emergency department with acute abdominal pain and amenorrhea. The vaginal ultrasound showed a right para‐adnexal mass with a living embryo. An abdominal pregnancy on the ascending colon was found in the laparotomy. The management included removing the gestational sac and treatment with methotrexate.
Iterative re‐weighted covariates selection for robust feature selection modelling in the presence of outliers (irCovSel)
Authors: Mishra Puneet , Liland Kristian Hovde 
Published: November 2022

AbstractA new method for feature selective modelling in the presence of outliers is presented. The method is a combination of iterative re‐weighted partial least squares and the covariates selection approach. The method relies on iterative down‐weighting of the outlying samples prior to estimating the squared covariance for covariates selection. In this way, the outlying samples carrying low weights have minimal influence on the squared covariance estimation, while the inliers have the maximum influence on the squared covariance estimation. The method allows selecting robust features, and models based on such features in general perform better in terms of prediction accuracy (lower error) than selecting features using equal sample weights for all samples. The algorithm description and tests of the method in single and multiple response scenarios are presented. Method performance is also demonstrated on a real spectral data set.
Kinetic study of crude oil‐to‐chemicals via steam‐enhanced catalytic cracking in a fixed‐bed reactor
Authors: Xu Qi , Akah Aaron C. , AlHerz Mansour , Aitani Abdullah , Qureshi Ziyauddin S. , Siddiqui M. Abdul Bari , Abo‐Ghander Nabeel 
Published: November 2022

AbstractThis study presents new experimental results on the direct conversion of crude oil to chemicals via steam‐enhanced catalytic cracking. We have organized the experimental results with a kinetics model using crude oil and steam co‐feed in a fixed‐bed flow reactor at reaction temperatures of 625, 650, and 675°C over the Ce‐Fe/ZSM‐5 catalyst. The model let us find optimum conditions for crude oil conversion, and the order of the steam cracking reaction was 2.0 for heavy oil fractions and 1.0 for light oil fractions. The estimated activation energies for the steam cracking reactions ranged between 20 and 200 kJ/mol. Interestingly, the results from kinetic modelling helped in identifying a maximum yield of light olefins at an optimized residence time in the reactor at each temperature level. An equal propylene and ethylene yield was observed between 650 and 670°C, indicating a transition from dominating catalytic cracking at a lower temperature to a dominating thermal cracking at a higher temperature. The results illustrate that steam‐enhanced catalytic cracking can be utilized to effectively convert crude oil into basic chemicals (52.1% C2‐C4 light olefins and naphtha) at a moderate severity (650°C) as compared to the conventional high‐temperature steam cracking process.
Optimization of deep learning models for the prediction of gene mutations using unsupervised clustering
Authors: Chen Zihan , Li Xingyu , Yang Miaomiao , Zhang Hong , Xu Xu Steven 
Published: November 2022

AbstractDeep learning models are increasingly being used to interpret whole‐slide images (WSIs) in digital pathology and to predict genetic mutations. Currently, it is commonly assumed that tumor regions have most of the predictive power. However, it is reasonable to assume that other tissues from the tumor microenvironment may also provide important predictive information. In this paper, we propose an unsupervised clustering‐based multiple‐instance deep learning model for the prediction of genetic mutations using WSIs of three cancer types obtained from The Cancer Genome Atlas. Our proposed model facilitates the identification of spatial regions related to specific gene mutations and exclusion of patches that lack predictive information through the use of unsupervised clustering. This results in a more accurate prediction of gene mutations when compared with models using all image patches on WSIs and two recently published algorithms for all three different cancer types evaluated in this study. In addition, our study validates the hypothesis that the prediction of gene mutations solely based on tumor regions on WSI slides may not always provide the best performance. Other tissue types in the tumor microenvironment could provide a better prediction ability than tumor tissues alone. These results highlight the heterogeneity in the tumor microenvironment and the importance of identification of predictive image patches in digital pathology prediction tasks.
A classy energy efficient spider monkey optimization based clustering and data aggregation models for wireless sensor network
Authors: Arunachalam Gnana Soundari , S Vimal , Ramalingam Gomathi , Nanjappan Rajendran 
Published: November 2022

SUMMARYEstablishment of energy efficient and reliable data routing in wireless sensor network (WSN) is one of the most critical and challenging task in the recent days. Also, the overall performance and lifetime of WSN is highly depends on the energy level of sensor nodes, hence it is most essential to save the energy of network. For this purpose, the different types of clustering and data aggregation mechanisms are developed in the conventional works, which are focusing on improving both the energy conservation and lifetime of network. Yet, it facing the challenges of increased computational complexity, inefficient routing of data, high controlling overhead, and reduced reliability. Thus, the proposed work objects to develop a novel energy efficient mechanism by integrating the functionalities of advanced clustering, path selection, and data aggregation methodologies. Here, the spider monkey optimization based energy efficient routing protocol is developed for optimally selecting the cluster head (CH) based on certain parameters of energy, distance, and weight value. In this framework, the data transmission is performed between the source to destination nodes through the relay nodes and CHs, which helps to minimize the energy consumption of network. Then, the Classy Bellman‐Ford algorithm is deployed for identifying the best paths having shortest distance with the sink nodes. Consequently, an anticipated data aggregation mechanism is utilized for ensuring the security and reliability of data transmission in WSN. For evaluation assessment, various performance metrics have been utilized to validate the results of proposed methodology, and also the obtained values are compared with some other recent state‐of‐the‐art models for proving the betterment of proposed mechanism.
Improving Solubility and Avoiding Hygroscopicity of Tetrahydropalmatine by Forming a Pharmaceutical Salt Cocrystal via CAHBs
Authors: Zhang Yunan , Shi Jingwen , Liu Lixin , Su Xin , Peng Bihui , Sun Weitong , Li Jinjing , Feng Yanru , Geng Yiding , Cheng Guangdong 
Published: November 2022

AbstractIn order to improve the solubility of tetrahydropalmatine (THP), an unreported THP‐sulfosalicylic acid (THP‐SSA) pharmaceutical salt cocrystal is designed and synthesized. Structural analysis shows that the building unit of THP‐SSA is composed of two THP cations, two SSA anions and one ethyl acetate molecule. Meanwhile, 1D chain, 2D layered structure, 3D hydrogen bond network, and Supramolecular motif R1110(48) of THP‐SSA are connected by stable charge‐assisted hydrogen bonds (CAHBs) N+‐H···O‐, π–π packing, and hydrogen bond. As expected, the maximum solubility of THP‐SSA in water is about 60 times that of THP, probably due to the protonation of the N atom on the piperidine ring in THP forms a pharmaceutical salt cocrystal, which greatly improves the solubility of THP. However, to the surprise, the hygroscopicity of THP‐SSA is also effectively reduced compared with THP, probably because the hydrogen bonds between THP and SSA have been formed, preventing the entry of water molecules. In addition, THP‐SSA also has higher antibacterial activity than THP. This suggests that forming a pharmaceutical salt cocrystal by cocrystal technology can not only improve the solubility of THP and avoid the hygroscopic property, but also improve the antibacterial activity of THP in vitro.
Glasgow‐Blatchford score combined with nasogastric aspirate as a new diagnostic algorithm for patients with nonvariceal upper gastrointestinal bleeding
Authors: Wakatsuki Toshiyuki , Mannami Tomohiko , Furutachi Shinichi , Numoto Hiroki , Umekawa Tsuyoshi , Mitsumune Mayu , Sakaki Tsukasa , Nagahara Hanako , Fukumoto Yasushi , Yorifuji Takashi , Shimizu Shin'ichi 
Published: November 2022

AbstractObjectivesThe Glasgow‐Blatchford score (GBS) is a widely used risk assessment tool for patients with upper gastrointestinal bleeding. However, it only identifies a relatively low proportion of patients at low risk for adverse events and poor outcomes. We developed a simple diagnostic algorithm combining the GBS and nasogastric aspirate and evaluated its diagnostic performance.MethodsA total of 115 consecutive patients with suspected nonvariceal upper gastrointestinal bleeding who underwent nasogastric tube placement and upper endoscopy at our emergency department were prospectively evaluated. We compared the diagnostic accuracy of the GBS and our algorithm for predicting high‐risk endoscopic lesions (HRELs) using receiver operating characteristic curve analysis.ResultsThirty‐five patients had HRELs. Compared with the GBS, our algorithm showed superior performance with respect to the prediction of HRELs (area under the curve, 0.639 and 0.854, respectively; p < 0.001). With set optimal threshold values, the algorithm identified a significantly higher proportion of patients who did not have HRELs than the GBS (23.5% vs. 2.6%, p < 0.001).ConclusionsThe novel algorithm has improved the diagnostic performance of the GBS and predicted more patients who did not have HRELs than the GBS alone. After further validation, it may be a useful tool for making clinical management decisions for patients with nonvariceal upper gastrointestinal bleeding.
Outpatient treatment of worsening heart failure with intravenous diuretics: first results from a multicentre 2‐year experience
Authors: Wierda Eric , van Maarschalkerwaart Willemijn (W.A.) , van Seumeren Esther , Dickhoff Cathelijne , Montanus Irina , de Boer Dominique , Kop Esther , de Mol Bas A.J.M. , Schroeder‐Tanka Jutta M. , van Heerbeek Loek 
Published: November 2022

AbstractAimsThe aim of this study is to examine the safety and efficacy of outpatient treatment of worsening heart failure (WHF) with intravenous diuretics.Methods and resultsThis is a multicentre retrospective observational research study. Patients with all types of heart failure (HF) were included: heart failure with reduced ejection fraction (HFrEF), heart failure with mildly reduced ejection fraction (HFmrEF), and heart failure with preserved ejection fraction (HFpEF). Patients included in this study were 18 years or older, had symptoms of WHF, had weight gain of more than 2 kg, and were not responding to uptitrating of oral diuretic therapy. Patients were treated for one or more days at the outpatient department with administration of intravenous loop diuretics with or without a bolus. In this study, 259 patients were included (mean age of 76 years, mean left ventricular ejection fraction of 41%). Rehospitalization rates for HF were 30.5% and 53.3%, respectively, at 30 days and 1 year. All‐cause mortality was 5.8% and 26.3%, respectively, at 30 days and 1 year. Rehospitalization rates for HF and all‐cause mortality were highest in patients with HFrEF. In a total of 322 individual outpatient treatments with intravenous diuretics, only one adverse event was registered.ConclusionsOutpatient treatment with intravenous diuretics of patients with WHF is a safe alternative strategy compared with the same treatment in hospitalized patients. However, only non‐randomized data are available and rehospitalization rates for this group with WHF are high. No data are available on the best selection criteria and the cost‐effectiveness of outpatient treatment with intravenous diuretics.
The Perfluoroalkylthiolation Reaction of Indoles and Activated Arenes with Perfluoroalkanesulfenic Acids
Authors: Li Jia‐Hui , Xu Jia‐Hong , Jiang Min , Song Li‐Ping , Liu Jin‐Tao 
Published: November 2022

AbstractThe perfluoroalkylthiolation reaction of indoles with perfluoroalkanesulfenic acids was achieved under mild conditions, while CH3COOH or TfOH was used as additive to promote the perfluoroalkylthiolation of activated benzene and pyrrole derivatives. A series of perfluoroalkylthiolated benzenes and heteroaromatic compounds were synthesized in moderate to good yields under transition‐metal‐free conditions.