bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeted therapies in CLL/SLL and the cumulative incidence of infection: A systematic review and meta-analysis
Authors: Vassilopoulos Stephanos , Shehadeh Fadi , Kalligeros Markos , Tran Quynh-Lam , Schiffman Fred , Mylonakis Eleftherios 
Published: September 2022

Background: Patients with chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL) are prone to infections.Aims: Provide a pooled estimate of the cumulative incidence for infections that fulfilled the criteria associated with severe infectious adverse events for grade 3 or higher (including pneumonia, febrile neutropenia and sepsis) in patients who receive targeted therapies.Methods: We searched PubMed and EMBASE for randomized controlled trials (RCT) that included patients with CLL/SLL who received targeted therapies and performed a random-effects meta-analysis to estimate the cumulative incidence of infections.Results: Of 2,914 studies screened, we retrieved 31 which evaluated 11,660 patients. The pooled cumulative incidence of infections for patients who received treatment regimens based on a BTK inhibitors was 19.86%. For patients who received treatment based on rituximab and second generation anti-CD20 monoclonal antibodies, the pooled cumulative incidence of infections was 19.85 and 13.46%, respectively. Regarding PI3K inhibitor-based regimens the cumulative incidence of severe infections was 30.89%. BCL-2 inhibitors had a cumulative incidence of infections of 17.49% while lenalidomide and alemtuzumab had an incidence of 13.33 and 45.09%, respectively. The cumulative incidence of pneumonia ranged from 3.01 to 8.45% while febrile neutropenia ranged from 2.68 to 10.80%. Regarding sepsis, the cumulative incidence ranged from 0.9 to 4.48%.Conclusion: Patients with CLL/SLL who receive targeted therapies may develop severe infections at significant rates that, in addition to disease stage and other complications, depend on the mechanism of action of the used drug. Surveillance for infections and development of effective prophylactic strategies are critical for patients with CLL/SLL who receive targeted therapies.Systematic Review Registration: [https://systematicreview.gov/], identifier [registration number]
Identification of lncRNA DLEU2 as a potential diagnostic biomarker and anti-inflammatory target for ulcerative colitis
Authors: Lin Qiuling , Zhang Dingguo , Zhang Jian , Luo Weixiang , Xu Zhenglei , Yao Jun , Wang Lisheng 
Published: September 2022

The incidence of ulcerative colitis (UC) in China has significantly increased over the past 10 years. Here we aim to explore potential diagnostic biomarkers and anti-inflammatory targets associated with UC. Patients with UC were enrolled in this study. The expression of lncRNAs and mRNAs in the nidus of the gut mucosa and adjacent normal mucosa samples was evaluated by RNA sequencing. The role of DLEU2 in inflammation and NF-κB signaling pathway was examined by RT-qPCR, Western blotting, and ELISA with human macrophage-like cells derived from THP-1. 564 lncRNAs and 859 mRNAs are significantly altered in the nidus of the gut mucosa of UC patients. Among the differentially expressed lncRNAs, DLEU2 changes the most. The expression of DLEU2 is negatively associated with inflammatory factors such as TNF-α, IL-1α, IL-1β, IL-6, and NLRP3. Mechanistically, DLEU2 exerts anti-inflammatory activity by inhibiting the NF-κB signaling pathway. In conclusion, the lncRNA DLEU2 in the intestinal mucosa is dysregulated upon gut inflammation and may act as a diagnostic biomarker and a therapeutic target for UC.
Incremental prognostic value of lung ultrasound on contemporary heart failure risk scores
Authors: Maestro-Benedicto Alba , Rivas-Lasarte Mercedes , Fernández-Martínez Juan , López-López Laura , Solé-González Eduard , Brossa Vicens , Mirabet Sonia , Roig Eulàlia , Cinca Juan , Álvarez-García Jesús , Sionis Alessandro 
Published: September 2022

Introduction: Over the last decades, several scores have been developed to aid clinicians in assessing prognosis in patients with heart failure (HF) based on clinical data, medications and, ultimately, biomarkers. Lung ultrasound (LUS) has emerged as a promising prognostic tool for patients when assessed at discharge after a HF hospitalization. We hypothesized that contemporary HF risk scores can be improved upon by the inclusion of the number of B-lines detected by LUS at discharge to predict death, urgent visit, or HF readmission at 6- month follow-up.Methods: We evaluated the discrimination improvement of adding the number of B-lines to 4 contemporary HF risk scores (Get with the Guidelines -GWTG-, MAGGIC, Redin-SCORE, and BCN Bio-HF) by comparing the change in the area under the receiver operating curve (AUC), the net reclassification index (NRI), and the integrated discrimination improvement (IDI). The population of the study was constituted by the 123 patients enrolled in the LUS-HF trial, adjusting the analyses by the intervention.Results: The AUC of the GWTG score increased from 0.682 to 0.789 (p = 0.02), resulting in a NRI of 0.608 and an IDI of 0.136 (p &lt; 0.05). Similar results were observed when adding the number of B-lines to the MAGGIC score, with an AUC that increased from 0.705 to 0.787 (p &lt; 0.05). This increase translated into a NRI of 0.608 and an IDI of 0.038 (p &lt; 0.05). Regarding Redin-SCORE at 1-month and 1-year, the AUC increased from 0.714 to 0.773 and from 0.681 to 0.757, although it did not reach statistical significance (p = 0.08 and p = 0.06 respectively). Both IDI and NRI were significantly improved (0.093 and 0.509 in the 1-month score, p &lt; 0.05; 0.056 and 0.111 in the 1-year score, p &lt; 0.05). Lastly, the AUC for the BCN Bio-HF score increased from 0.733 to 0.772, which was statistically non-significant, with a NRI value of 0.363 (p = 0.06) and an IDI of 0.092 (p &lt; 0.05).Conclusion: Adding the results of LUS evaluated at discharge improved the predictive value of most of the contemporary HF risk scores. As it is a simple, fast, and non-invasive test it may be recommended to assess prognosis at discharge in HF patients.
A pilot investigation of the efficacy and safety of magnesium chloride and ethanol as anesthetics in Loligo vulgaris embryos
Authors: Sprecher Marta , Sprecher Simon G. , Spadavecchia Claudia 
Published: September 2022

The inclusion of cephalopods in the legislation related to the use of animals for experimental purposes has been based on the precautionary principle that these animals have the capacity to experience pain, suffering, distress, and lasting harm. Recent studies have expanded this view and supported it. Handling cephalopod mollusks in research is challenging and whenever more invasive procedures are required, sedation and/or anesthesia becomes necessary. Therefore, finding adequate, safe, and effective anesthetics appears mandatory. Several substances have been considered in sedating cephalopods, in some instances applying those utilized for fish. However, species-specific variability requires more detailed studies. Despite long-lasting experience being linked to classic studies on squid giant axons, evidence of action on putative anesthetic substances is scarce for Loligo vulgaris and particularly for their embryos. The aim of the current study was to evaluate effects elicited by immersion of squid embryos in anesthetic solutions and examine whether these forms display a similar reaction to anesthetics as adults do. Different concentrations of ethanol (EtOH; 2, 2.5, and 3%) and magnesium chloride (MgCl2; 1, 1.5, and 1.8%) were tested by adopting a set of indicators aimed at exploring the physiological responses of squid embryos. Forty-two embryos of the common squid Loligo vulgaris (stages 27–28) were assigned to three conditions (EtOH, MgCl2, and controls) and video recorded for 15 min (5 min before, 5 min during, and 5 min after immersion in the anesthetic solutions). In each group, the heart rate, respiratory rate, buoyancy, chromatophore activity, and tentacles/arms responses were assessed to evaluate the embryos' vitality and responsiveness to stimulation. Both substances provoked a decrease in heart and respiratory rates and inhibited buoyancy, chromatophores, and tentacles/arms responses; no adverse effects were observed. EtOH had a faster onset of action and faster recovery than MgCl2, being potentially more adequate as an anesthetic for shorter procedures. Even though MgCl2 caused a longer muscle relaxation, the reversibility was not confirmed for the 1.8% concentration; however, lower concentrations triggered similar results as the ones obtained with the highest EtOH concentrations. We have shown that the late developmental stages of Loligo vulgaris embryos could represent a good model to evaluate anesthetics for cephalopods since they can display similar reactions to anesthetics as adults animals do.
Deadly scents: Exposure to plant volatiles increases mortality of entomopathogenic nematodes during infection
Authors: Gaffke Alexander M. , Shapiro-Ilan David , Alborn Hans T. 
Published: September 2022

Plants attacked by insects commonly mobilize various defense mechanisms, including the biosynthesis and release of so-called herbivore-induced plant volatiles (HIPVs). Entomopathogenic nematodes (EPNs) can be attracted to these belowground HIPVs, which can enhance biocontrol services from EPNs. However, recent research has also demonstrated that HIPVs can induce and initiate insect immune responses, decreasing the insect’s susceptibility to pathogens and parasites. Therefore, experiments were conducted to test the impact of HIPVs on insects and EPNs during the initial stage of EPN infection. Compounds that can impact EPN attraction and infectivity such as pregeijerene, β-caryophyllene, and α-pinene, and compounds that have been determined to increase or decrease susceptibility of insects to pathogens, such as (Z)-3-hexenyl acetate, linalool, and β-ocimene, were selected. Exposure of Galleria mellonella larvae to pregeijerene, linalool, β-ocimene and α-pinene during invasion significantly increased mortality of Steinernema diaprepesi and Heterorhabditis bacteriophora after 48 h. Larval treatment with β-caryophyllene only increased mortality for Heterorhabditis bacteriophora. (Z)-3-hexenyl acetate did not cause differential mortality from the controls for either nematode species. In additional experiments, we found that EPNs exposed to α-pinene and linalool were more readily recognized by the insects’ immune cells compared to the control treatment, thus the observed increased mortality was likely due to HIPVs-EPN interactions with the insect’s immune system. These results show that the presence of HIPVs can impact EPN survival in the model host, G. mellonella.
Your health is in your mouth: A comprehensive view to promote general wellness
Authors: Barranca-Enríquez Antonia , Romo-González Tania 
Published: September 2022

BackgroundEven though various studies recognize the importance of the oral cavity to have general health, in multidisciplinary professional practice it is almost always excluded and on an individual basis, very commonly neglected. Oral diseases are preventable, still, they are highly prevalent. Although some studies consider oral health within integral health, currently, there is no model in which the mouth is integrated within other levels for the achievement of well-being. The objective of this article was to review the importance of oral health and its connection with well-being and, based on these findings, propose a complex and comprehensive perspective for approach and care.MethodsThe databases MEDLINE, PubMed, and Google Scholar were revised for randomized controlled trials and reviews that included search terms related to oral health and its relationship with the general health in its different levels (physical, psychological, social and environmental).ResultsThe review shows that oral health is critical, as the teeth and mouth are not only an integral part of the body, but also, they also support and enable essential human functions. That is, oral health has a multidimensional nature, as it includes the physical, psychological, social, and environmental domains that are essential for overall health and well-being. Likewise, the mouth is the psychological seat of the first physiological needs and emotional gratifications, with it we take a taste of the world around us. Thus, the mouth plays an important role in the feeling of unity and in the constitution of the self. Based on these results we propose an integrative model in which the mouth is the first step for well-being and from this integrative model we build a multidisciplinary approach which could be used in the clinical practice for the promotion of oral care and general health.ConclusionThe effort on the part of oral health professionals is essential for people's well-being and must be integrated as part of health promotion. Dental treatments alone cannot solve this problem, it requires a comprehensive and approach in which the bio-psychological, behavioral, and socio-environmental determinants are included to face this global oral health challenge. That is, without a comprehensive and multidisciplinary approach to medical science that includes dental and oral health, our public policies cannot provide the best answers to health promotion, disease prevention, early detection, and treatment.
Antibiotic consumption and time to recovery from uncomplicated urinary tract infection: secondary analysis of observational data from a point-of-care test trial
Authors: Gadalla Amal , Wise Hannah , Farewell Daniel , Hughes Kathryn , Llor Carl , Moore Michael , Verheij Theo JM , Little Paul , Butler Christopher C , Francis Nick A 
Published: September 2022

BackgroundRandomised trials provide high-quality evidence on the effects of prescribing antibiotics for urinary tract infection (UTI) but may not reflect the effects in those who consume antibiotics. Moreover, they mostly compare different antibiotic types or regimens but rarely include a ‘no antibiotic’ group.AimTo estimate the effect of antibiotic consumption, rather than prescription, on time to recovery in females with uncomplicated UTI.Design and settingSecondary analysis of 14-day observational data from a point-of-care test trial for UTI in primary care in England, the Netherlands, Spain, and Wales, which ran from 2012 to 2014. Clinicians treated patients using their own judgement, providing immediate, delayed, or no antibiotic.MethodUTI-symptomatic females who either consumed or did not consume antibiotics during a 14-day follow-up were included. Antibiotic consumption was standardised across participants and grouped into either ≤3 or >3 standardised antibiotic days. To account for confounders, a robust propensity score matching analysis was conducted. Adjusted Kaplan–Meier and Cox proportional hazard models were employed to estimate time to recovery and hazard ratios, respectively.ResultsA total ofn= 333 females who consumed antibiotics andn= 80 females who did not consume antibiotics were identified and included in the study. The adjusted median time to recovery was 2 days longer among patients who did not consume antibiotics (9 days, 95% confidence interval [CI] = 7 to 12) compared with those who did (7 days, 95% CI = 7 to 8). No difference was found between those who consumed ≤3 (7 days, 95% CI = 7 to 8) compared with >3 standardised antibiotic days (7 days, 95% CI = 6 to 9).ConclusionConsuming antibiotics was associated with a reduction in self-reported time to recovery, but more antibiotics exposure was not associated with faster recovery in this study.
Pendampingan Pastoral bagi Pasangan yang Bercerai
Authors: Setiawan Tjutjun , Lasmaria Fitry Riny , Hermanto Yanto Paulus , Utomo Karyo 
Published: September 2022

Setiap orang pasti tidak menginginkan bahtera rumah tangganya hancur dan berujung dengan perceraian. Dalam realita hidup banyak dijumpai pasangan-pasangan yang melakukan perceraian dengan berbagai alasan dan itu memberikan dampak bukan hanya terhadap anak-anak, keluarga tetapi juga terhadap pasangan yang bercerai tersebut. Gereja pada umumnya memberikan pendampingan pada waktu pasangan menikah itu hendak bercerai, tetapi setelah keluarnya putusan pengadilan yang mensahkan perceraian mereka, pendampingan pastoral tidak lagi dilakukan. Penelitian ini mengkaji bagaimana pentingnya pendampingan pastoral bagi pasangan bercerai sehingga dapat mengurangi dampak dalam kehidupan mereka yang sedang dalam pra-perceraian ataupun pasca perceraian. Metode yang digunakan adalah studi kepustakaan dan menggunakan instrumen wawancara dengan salah satu pasangan yang bercerai, dan kesimpulan yang didapat adalah betapa penting gereja melakukan pendampingan pastoral bagi pasangan yang melakukan perceraian agar mereka tetap dapat menjalankan dan melanjutkan kehidupan sehari-hari dan membangun kehidupan rohani dengan lebih baik lagi, mencegah terjadinya perzinahan karena kebutuhan biologis yang tidak tersalurkan sebagai konsekuensi logis dari sebuah perceraian, serta tidak tertutup kemungkinan pasangan bercerai ini diarahkan untuk dapat rujuk kembali.apabila mereka tidak tahan bertarak. Kata-kata kunci: Perceraian; pendampingan pastoral; perkawinan Kristen
Dosa Anak Lembu Emas dan Citra Diri Harun: Refleksi Kajian Biblis Keluaran 32:1-35 tentang Kepemimpinan Kristiani
Authors: Joswanto Andreas , Anjaya Carolina Etnasari , Arifianto Yonatan Alex 
Published: September 2022

Kontribusi Harun dalam membangun patung tuangan anak lembu emas menjadi preseden buruk bagi kepemimpinan Kristen. Harun memiliki anugerah pengalaman, kesempatan dan kemampuan untuk menghindarkan bangsa Israel dari dosa besar, namun dia tidak melakukannya. Artikel ini bertujuan mendalami makna tindakan Harun dalam peristiwa patung tuangan anak lembu, memberikan analisis terhadapnya dan memberikan deskripsi refleksi peristiwa tersebut terhadap kepemimpinan Kristen saat ini. Metode artikel menggunakan kualitatif melalui sebuah pendekatan studi literatur tentang kepemimpinan Harun dan refleksinya. Hasil simpulan ditemukan bahwa kesalahan Harun diakibatkan karena motivasi kepemimpinan didasarkan kepada citra diri yang berujung pada sikap kompromi terhadap kebenaran atau tidak memiliki keberanian dalam menentang ketidakbenaran. Dampak dari hal tersebut adalah perbuatan dosa di mata Tuhan dan hukuman Tuhan atasnya.
The Development of Edu-Detective Thinking Learning Model For Biology Students
Authors: Priyambodo Panggih , Saputri Wulandari , Winarto Winarto , Firdaus Firdaus 
Published: September 2022

This study aims to: (1) produce an appropriate Edu-Detective Thinking learning model, (2) produce a practical Edu-Detective Thinking learning model, and (3) produce an effective Edu-Detective Thinking learning model to improve environmental character, science processes skill, and students' cognitive processing abilities. This type of Research Development research refers to the Borg Gall development model, which includes: (1) preliminary study and information gathering, (2) planning, (3) initial product development, (4) preliminary field testing, (5) product revision main field, (6) main field testing, (7) operational product, (8) operational field testing, (9) final product revision, (10) dissemination implementation. Data on product validity and practicality were collected using questionnaires and observation sheets. Then, the data were analyzed descriptively quantitatively. Meanwhile, the effectiveness data were collected by test questions and analyzed using the MANOVA test. The results of the study are as follows: (1) the Edu-Detective Thinking learning model is feasible to improve the environmental character, science process skills, and students' cognitive processing abilities, (2) the practical Edu-Detective Thinking learning model to improve environmental character, science process skills, and students' cognitive processing abilities, and (3) the Edu-Detective Thinking learning model is effective for improving the environmental character, science process skills, and students' cognitive processing abilities. Thus, it can be concluded that the Edu-Detective Thinking model can be an alternative learning model to improve the quality of biology learning to be more challenging and fun for students.
Implementation of Online Teaching During the Covid-19 Pandemic: Teachers’ Experiences
Authors: Hidayat Rintani , Syamsi Kastam 
Published: September 2022

This study aims to analyze the learning process online, how is the level of readiness of teachers in organizing online learning, how is the level of readiness of students in receiving online learning. The supporting factors and inhibiting in implementing the courage system learning daring the COVID-19 pandemic. This study was conducted at one of Senior Hight School in Merangin. This research is qualitative. The data subjects in the study were all Indonesian language teachers at Senior Hight School in Merangin totaling six teachers. The data collection techniques in this study were in the form of observation and interviews. The collected data is then processed and analyzed to be presented in the form of narrative paragraphs. The data analysis process begins with data reduction, data analysis, interpretation, and conclusion. The results of this study reveal that online learning has been carried out quite well by Indonesian language teachers during the COVID-19 pandemic. This is because the teacher has prepared lesson plans, learning activities, and learning evaluations. The benefits of online learning that most teachers feel are increased competence in using technology for learning. Teachers’ adaptations to the implementation of online learning during the COVID-19 pandemic include gathering information to increase their understanding of online learning through internet media such as Google and YouTube. However, online learning has constraints in the implementation of which is unstable tissue conditions and learners’ difficulties in understanding learning materials are unique challenges in online learning.
E-Test: Alternative Online Assessment for Online Learning Classes in Vocational High Schools
Authors: Rahayu Sri , Friyatmi Friyatmi 
Published: September 2022

This study aims to develop a moodle – based online test to asses the student learning outcome in vocational high school. It is expected to help and facilitate the teacher in correcting out the evaluation of learning at school. This study was research and development which in included 4 stages : define, design, develop and disseminate. The subjeck of this research is accounting and instititional finance majoring students at grade XI. The results of the research the Moodle-based online test which was developed based on the results of validation by IT experts obtained a score of 5 with the criteria of 'Very Good' for the display aspect and for the programming aspect. Meanwhile, for the practicality test, the application obtained a score of 88% which was in the range of 81%-100%. So it can be concluded that the Moodle online test application which was developed is "Very Good" so that, it can be used to carry out the learning evaluation activities