bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Surgical Margins with Intraoperative Frozen Section in Patients Undergoing Breast-Conserving Surgery: Our clinical experience.
Authors: Koca Bulent , Yıldırım Murat , Saglam Ali İhsan 
Published: July 2022

Background: Breast cancer is the second most common cancer in women. Breast-conserving surgery (BCS) has increasingly been applied to eligible patients. One of the most important points in BCS is to provide clean surgical margins. In this study, it is aimed to share the re-excision results of those having positive surgical margins in intra-operative evaluation among BCS patients, and to discuss the intra-operative evaluation in the light of the literature. MATERIALS AND METHODS:The data (patient files, surgery notes and pathology reports) of 203 patients who had undergone BCS for breast cancer between January 2016 and January 2022 and whose excision materials had been evaluated with intra-operative frozen sections in terms of surgical margins, were scanned retrospectively. Absence of ink on tumoral cells (“no ink on tumor”) for invasive ductal carcinoma (IDC), and a margin width of ≥ 2mm for ductal carcinoma in situ (DCIS) were considered as a clean surgical margin.RESULTS: Median age was 51 years (range, 22-75). There were reportedly 27 with positive surgical margins, five of whom with positive margins in re-excision. Second re-excision specimens of all these 5 cases (18.5%) had been reported to have no positive surgical margins. No patients had been reported to require a second re-excision or mastectomy.CONCLUSION: Intraoperative surgical margin assessment eliminates the need for repetitive surgery. We recommend routine application of intra-operative surgical margin assessment.
HUBUNGAN DUKUNGAN KELUARGA DENGAN KEPATUHAN DIET RENDAH GARAM PADA PENDERITA HIPERTENSI SISTOLIK
Authors: InaKii Maria , Soelistyoningsih Dwi , Jayanti Nicky Danur 
Published: July 2022

Hipertensi sistolik merupakan kondisi tekanan darah sistolik >140 mmHg dan tekanan darah diastolik <90 mmHg. Kepatuhan diet rendah garam memerlukan waktu yang cukup lama sehingga dibutuhkan dukungan keluarga agar tercapainya kepatuhan diet rendah garam pada pasien Hipertensi. Tujuan penelitian ini adalah menganalisis hubungan dukungan keluarga dengan kepatuhan diet rendah garam pada penderita hipertensi sistolik di Puskesmas Dinoyo Kota Malang. Metode berupa penelitian deskriptif korelasi dengan pendekatan cross sectional. Sampel penelitian sebesar 52 responden menggunakan teknik accidental sampling, dengan kriteria inklusi responden usia >18 tahun, pasien hipertensi sistolik dalam tiga bulan terakhir di Puskesmas Dinoyo Kota Malang. Instrumen penelitian menggunakan kuisioner dan dianalisis dengan uji kendall’s tau-b. Hasil penelitian didapatkan 22 responden memiliki dukungan keluarga dalam kategori baik (42,3%), memiliki kepatuhan diet rendah garam sebanyak 30 (57,2%) responden. Dari hasil uji statistic didapatkan hubungan signifikan (p=0,000) dukungan keluarga dengan kepatuhan diet rendah garam pada penderita hipertensi sistolik di Puskesmas Dinoyo Kota Malang, dengan kekuatan korelasi sedang (r= 0,467). Kesimpulan dari penelitian ini adalah terdapat hubungan dukungan keluarga dengan kepatuhan diet rendah garam di Puskesmas Dinoyo Kota Malang, sehingga penting untuk meningkatkan kepatuhan program diet rendah garam penderita hipertensi
GAGASAN HAK INGKAR DALAM PELAKSANAAN JABATAN NOTARIS DI INDONESIA: STUDI ANALISIS DAMPAK PUTUSAN MAHKAMAH KONSTITUSI NO. 49/PUU.X/2012
Authors: Fidhira Fitrah , Ryan Bakry Mohammad , Yusuf Chandra 
Published: July 2022

Notaris dalam menjalankan jabatannya berdasarkan pasal 16 ayat (1) huruf f Undang-Undang Nomor 30 Tahun 2004 mempunyai kewajiban untuk merahasiakan isi Akta, Putusan Mahkamah Konstitusi Nomor 49/PUU.X/2012 memberikan dasar bahwa pasal 66 bertentangan dengan Undang-Undang Dasar Pasal 27 ayat (1) dan Pasal 28D (1). Konsekuensi logis putusan ini yakni terbukanya argumentasi perihal konsep dan penerapan hukum hak ingkar dalam pelaksanaan jabatan Notaris. Penelitian ini menggunakan metode penelitian hukum normatif dengan pendekatan statute dan analytical jurisprudence. Analisis dilakukan terhadap Putusan Mahkamah Konstitusi Nomor 49/PUU.X/2012 dan Undang-Undang Jabatan Notaris Nomor 30 Tahun 2004. Hasil analisis menemukan bahwa: Pertama, Pasca dikeluarkannya Putusan Mahkamah Konstitusi Nomor 49/PUU.X/2012, maka Undang-Undang Jabatan Notaris Nomor 30 Tahun 2004 perihal hak ingkar, dapat dikecualikan jika berkaitan dengan due Process of law, akibat hukumnya adalah pemanggilan seorang Notaris tidak memerlukan lagi persetujuan Majelis Pengawas Daerah; Kedua, secara konseptual terdapat dua substansi utama yang menjadi argumentasi penting pasca- putusan Mahkamah Konstitusi Nomor 49/PUU.X/2012, yaitu prinsip equal before the law akan sejalan dengan prinsip due process of law, dan perlakuan yang berbeda terhadap jabatan Notaris yang mengedepankan peran Majelis Pengawas Daerah harus dipahami dalam kerangka Kode Etik Notaris, bukan pada tataran fungsi peradilan.
EFEKTIVITAS PELAKSANAAN KEBIJAKAN BERDASARKAN PERGUB NO 66 TAHUN 2020 TENTANG UJI EMISI KENDARAAN BERMOTOR DI JAKARTA
Authors: Michelle Elizabeth , Jusuf Melvin , Julian Jenni 
Published: July 2022

Di era modern saat ini banyak orang yang memilih pindah ke perkotaan hal ini mengakibatkan semakin padatnya daerah perkotaan dan yang paling nyata terlihat yaitu di jalan raya Tidak bisa dipungkiri bahwa kemacetan pasti selalu ada khususnya di ibukota Jakarta selain kemacetan polusi udara juga merupakan dampak dari padatnya penduduk. Efek dari polusi udara sangatlah buruk bagi kesehatan polusi udara dapat menimbulkan berbagai macam penyakit seperti gangguan pernapasan, jantung dan dapat menyebabkan kanker selain kesehatan polusi udara juga dapat menyebabkan pemanasan global.Salah satu penyebab utama polusi udara yang terjadi di Jakarta adalah dari kontribusi pembuangan gas emisi kendaraan bermotor hal ini dikarenakan banyak sekali masyarakat Indonesia terutama masyarakat di wilayah jakarta yang menggunakan kendaraan bermotor. Pemerintah Provinsi DKI Jakarta melihat permasalahan terkait buruknya kualitas udara di Jakarta karena itu dikeluarkan PERGUB NO 66 Tahun 2020 yang mengatur tentang uji emisi kendaraan bermotor. peraturan yang dikeluarkan oleh Pemerintah Daerah tersebut merupakan langkah yang sangat tepat demi memperbaiki kualitas udara yang ada di Jakarta ini. Oleh karena itu penelitian ini bertujuan untuk mengetahui efektivitas pelaksanaan uji emisi yang diatur di dalam PERGUB NO 66 Tahun 2020.
KEPASTIAN HUKUM DALAM PENYELESAIAN PELANGGARAN ETIKA RANGKAP JABATAN NOTARIS OLEH MAJELIS PENGAWAS DAERAH
Authors: Almuslimah Alidatussadiyah , Ryan Bakry Mohammad , Yusuf Chandra 
Published: July 2022

Dalam menjaga marwah suatu jabatan profesi notaris diperlukan adanya lembaga pengawas notaris. Lembaga ini dituntut untuk dapat menegakkan disiplin terhadap penyelenggaraan profesi notaris juga dalam hal penegakan hukum dalam terjadi pelanggaran kode etik notaris. Teknis pasti terhadap penindakan pelanggaran etik rangkap jabatan oleh Majelis Pengawas perlu dielaborasi agar menjadi jelas kepastian hukumnya. Penelitian ini memiliki tujuan untuk memahami dan menganalisis bagaimana peranan Majelis Pengawas Wilayah dalam penyelesaian pelanggaran etika rangkap jabatan menurut Undang-Undang Jabatan Notaris dan wujud impementasi kepastian hukum dalam putusan tindakan Majelis Pengawas Wilayah terhadap pelanggaran kode etik rangkap jabatan oleh notaris. Metode yang digunakan adalah metode pendekatan yuridis-normatif. Referensi yang digunakan dalam penelitian ini adalah data primer, buku-buku, jurnal, sumber dari media elektronik, dokumen akta, dan peraturan perundang-undangan serta peraturan kebijakan. Berdasarkan hasil penelitian, diketahui bahwa Majelis Pengawas Wilayah dalam penyelesaian pelanggaran etika rangkap jabatan terhadap notaris terikat pada wilayah yurisdiksinya. Terhadap notaris yang terbukti bersalah dapat diberikan sanksi peringatan tertulis, pemberhentian sementara, pemberhentian dengan hormat, hingga pemberhentian dengan tidak hormat. Wujud impementasi kepastian hukum dalam putusan tindakan Majelis Pengawas Wilayah dapat diukur melalui: benchmarking kejelasan aturan (jernih), konsisten, mudah diperoleh, diterbitkan oleh pemerintah, dan diakui
LOCUS OF CONTROL, TURNOVER INTENTION, KINERJA AUDITOR, ETIKA AUDITOR, KOMITMEN ORGANISASI DAN DYSFUNCTIONAL AUDIT BEHAVIOR
Authors: Medina Lusia Elvira , Challen Auliffi Ermian 
Published: July 2022

Penelitian ini bertujuan untuk menguji pengaruh Locus Of Control, Turnover Intention, Kinerja Auditor, Etika Auditor dan Komitmen Organisasi terhadap Dysfunctional Audit Behavior pada Kantor Akuntan Publik (KAP) di wilayah DKI Jakarta. Metode penentuan sampel dalam penelitian ini adalah dengan teknik purposive sampling berdasarkan pertimbangan. Responden dalam penelitian ini berjumlah 146 auditor di seluruh KAP yang berada di wilayah DKI Jakarta. Data yang digunakan adalah data primer berupa kuesioner yang dibagikan kepada responden.Penelitian ini diuji dengan menggunakan analisis linier regresi berganda. Berdasarkan hasil analisis dapat disimpulkan bahwa Etika Auditor berpengaruh positif terhadap Dysfunctional Audit Behavior dan Komitmen Organisasi berpengaruh negatif terhadap Dysfunctional Audit Behavior, sedangkan Locus Of Control, Turnover Intention dan Kinerja Auditor tidak berpengaruh terhadap Dysfunctional Audit Behavior.
Penggunaan Obat Asma Pada Pasien Asma di Puskesmas Karang Rejo Tarakan
Authors: Yuansafikri Reysaharif , Permana Dharma 
Published: July 2022

Latar belakangAsma adalah penyakit heterogen yang disebabkan pajanan alergen, ditandai dengan inflamasi jalan napas kronis dengan prevalensinya di Indonesia menurut Riset Kesehatan Dasar mencapai 4,5% dan untuk mengatasinya diperlukan obat – obat asma.TujuanPenelitian ini bertujuan untuk mengetahui penggunaan obat asma pada pasien asma di Puskesmas Karang Rejo Tarakan pada periode Januari – April 2017MetodeMetode penelitian yang dilakukan adalah deskriptif dengan mengumpulkan data sekunder dari rekam medik pasien asma memenuhi kriteria inklusi baru pertama kali mendapat terapi asma dengan data rekam medik yang lengkap.Hasil dan DiskusiJumlah pasien puskesmas sebanyak 26.947 dengan pasien yang memenuhi kriteria inklusi sebanyak 225 pasien terdiri dari laki – laki (56,8%) dan perempuan (43,2%) pada rentang usia terbanyak anak – anak usia 0-5 tahun (32,8%) dan usia 20-44 tahun (29,3%). Obat yang paling banyak digunakan adalah Salbutamol (42%) dan Dexsamethasone (21,2%). Monoterapi yang paling sering digunakan adalah Salbutamol (14,2%), kombinasi 2 obat yaitu Salbutamol dan Dexamethasone (27,1%), kombinasi 3 obat yaitu Salbutamol, Gliseril Guaiakolat, dan Dexsamethasone (13,7%), dan kombinasi 4 obat yaitu Salbutamol, Dexamethasone, Gliseril Guaiakolat dan Klorfeniramin Maleat (2,6%).KesimpulanSalbutamol merupakan obat utama dalam penanggulangan asma baik sebagai monoterapi, kombinasi 2 obat, kombinasi 3 obat, dan kombinasi 4 obat.
Differences in Linear Epitopes of Ara h 9 Recognition in Peanut Allergic and Tolerant, Peach Allergic Patients
Authors: Sánchez-Ruano L. , Fernández-Lozano C. , Ferrer M. , Gómez F. , de la Hoz B. , Martínez-Botas J. , Goikoetxea M. J. 
Published: July 2022

BackgroundPeanut-allergic patients from the Mediterranean region are predominantly sensitized to the lipid transfer protein (LTP) Ara h 9, and the peach LTP Pru p 3 seems to be the primary sensitizer. However, LTP sensitization in peanut allergy is not a predictive marker for clinically relevant symptoms.ObjectiveWe aimed to identify sequential epitopes of IgE and IgG4 from Pru p 3 and Ara h 9 in peach-allergic patients sensitized to peanuts. We also sought to determine the differences in IgE and IgG4 binding between patients who had developed peanut allergy and those tolerating peanuts.MethodsA total of 46 peach-allergic patients sensitized to peanuts were selected. A total of 35 patients were allergic to peanuts (peanut-allergic group) and 11 were tolerant to peanuts (peanut-tolerant group). We measured sIgE and sIgG4 in peanut, peach, and their recombinant allergen (Ara h 1, Ara h 2, Ara h 3, Ara h 8, and Ara h 9) with fluorescence enzyme immunoassay. We examined the IgE and IgG4 binding to sequential epitopes using a peptide microarray corresponding to linear sequences of the LTPs Ara h 9 and Pru p 3 with a library of overlapping peptides with a length of 20 amino acids (aa) and an offset of 3 aa.ResultsThe frequency and the intensity of IgE recognition of Ara h 9 and Pru p 3 peptides were higher in the peanut-tolerant group than in the peanut-allergic group. We found four Ara h 9 peptides (p4, p14, p21, and p25) and four Pru p 3 peptides (p1, p3, p21, and p24) with a significantly elevated IgE recognition in peanut-tolerant patients. Only one peptide of Ara h 9 (p4) recognized by IgG4 was significantly elevated in the peanut-tolerant group. The IgG4/IgE ratio of Ara h 9 peptide 4 was significantly higher in peanut-tolerant patients than in peanut-allergic patients, while no significant differences were observed in the IgG4/IgE ratio of this peptide in Pru p 3.ConclusionAlthough we found significant differences in IgE and IgG4 recognition of Ara h 9 and Pru p 3 between peanut-tolerant and peanut-allergic patients (all of whom were allergic to peach), polyclonal IgE peptide recognition of both LTPs was observed in peach-allergic patients tolerating peanuts. However, the IgG4 blocking antibodies against Ara h 9 peptide 4 could provide an explanation for the absence of clinical reactivity in peanut-tolerant peach-allergic patients. Further studies are needed to validate the usefulness of IgG4 antibodies against Ara h 9 peptide 4 for peanut allergy diagnosis.
Transfer mechanism of cell-free synthesized membrane proteins into mammalian cells
Authors: Umbach Simon , Levin Roman , Neumann Sebastian , Steinmetzer Torsten , Dötsch Volker , Bernhard Frank 
Published: July 2022

Nanodiscs are emerging to serve as transfer vectors for the insertion of recombinant membrane proteins into membranes of living cells. In combination with cell-free expression technologies, this novel process opens new perspectives to analyze the effects of even problematic targets such as toxic, hard-to-express, or artificially modified membrane proteins in complex cellular environments of different cell lines. Furthermore, transferred cells must not be genetically engineered and primary cell lines or cancer cells could be implemented as well. We have systematically analyzed the basic parameters of the nanotransfer approach and compared the transfer efficiencies from nanodiscs with that from Salipro particles. The transfer of five membrane proteins was analyzed: the prokaryotic proton pump proteorhodopsin, the human class A family G-protein coupled receptors for endothelin type B, prostacyclin, free fatty acids type 2, and the orphan GPRC5B receptor as a class C family member. The membrane proteins were cell-free synthesized with a detergent-free strategy by their cotranslational insertion into preformed nanoparticles containing defined lipid environments. The purified membrane protein/nanoparticles were then incubated with mammalian cells. We demonstrate that nanodiscs disassemble and only lipids and membrane proteins, not the scaffold protein, are transferred into cell membranes. The process is detectable within minutes, independent of the nanoparticle lipid composition, and the transfer efficiency directly correlates with the membrane protein concentration in the transfer mixture and with the incubation time. Transferred membrane proteins insert in both orientations, N-terminus in and N-terminus out, in the cell membrane, and the ratio can be modulated by engineering. The viability of cells is not notably affected by the transfer procedure, and transferred membrane proteins stay detectable in the cell membrane for up to 3 days. Transferred G-protein coupled receptors retained their functionality in the cell environment as shown by ligand binding, induction of internalization, and specific protein interactions. In comparison to transfection, the cellular membrane protein concentration is better controllable and more uniformly distributed within the analyzed cell population. A further notable difference to transfection is the accumulation of transferred membrane proteins in clusters, presumably determined by microdomain structures in the cell membranes.
Combining Metabolic Engineering and Multiplexed Screening Methods for 3-Hydroxypropionic Acid Production in Pichia pastoris
Authors: Fina Albert , Heux Stephanie , Albiol Joan , Ferrer Pau 
Published: July 2022

Production of 3-hydroxypropionic acid (3-HP) in Pichia pastoris (syn. Komagataella phaffii) via the malonyl-CoA pathway has been recently demonstrated using glycerol as a carbon source, but the reported metrics were not commercially relevant. The flux through the heterologous pathway from malonyl-CoA to 3-HP was hypothesized as the main bottleneck. In the present study, different metabolic engineering approaches have been combined to improve the productivity of the original 3-HP producing strains. To do so, an additional copy of the gene encoding for the potential rate-limiting step of the pathway, i.e., the C-terminal domain of the malonyl-CoA reductase, was introduced. In addition, a variant of the endogenous acetyl-CoA carboxylase (ACC1S1132A) was overexpressed with the aim to increase the delivery of malonyl-CoA. Furthermore, the genes encoding for the pyruvate decarboxylase, aldehyde dehydrogenase and acetyl-CoA synthase, respectively, were overexpressed to enhance conversion of pyruvate into cytosolic acetyl-CoA, and the main gene responsible for the production of the by-product D-arabitol was deleted. Three different screening conditions were used to classify the performance of the different strains: 24-deep-well plates batch cultures, small-scale cultures in falcon tubes using FeedBeads® (i.e., slow release of glycerol over time), and mini bioreactor batch cultures. The best two strains from the FeedBeads® screening, PpHP8 and PpHP18, were tested in bioreactor fed-batch cultures using a pre-fixed exponentially increasing feeding rate. The strain PpHP18 produced up to 37.05 g L−1 of 3-HP at 0.712 g L−1 h−1 with a final product yield on glycerol of 0.194 Cmol−1 in fed-batch cultures. Remarkably, PpHP18 did not rank among the 2-top producer strains in small scale batch cultivations in deep-well plates and mini bioreactors, highlighting the importance of multiplexed screening conditions for adequate assessment of metabolic engineering strategies. These results represent a 50% increase in the product yield and final concentration, as well as over 30% increase in volumetric productivity compared to the previously obtained metrics for P. pastoris. Overall, the combination of glycerol as carbon source and a metabolically engineered P. pastoris strain resulted in the highest 3-HP concentration and productivity reported so far in yeast.
Effect of biochar on antibiotics and antibiotic resistance genes variations during co-composting of pig manure and corn straw
Authors: Tong Zhenye , Liu Fenwu , Tian Yu , Zhang Jingzhi , Liu Hui , Duan Jiaze , Bi Wenlong , Qin Junmei , Xu Shaozu 
Published: July 2022

Pig manure is a reservoir of antibiotics and antibiotic resistance genes (ARGs). The effect of biochar on the variations in physicochemical properties, bacterial communities, antibiotics, ARGs, and mobile genetic elements (MGEs) of compost product during co-composting of pig manure and corn straw have been investigated in this study. Compared with the control treatment (CK), biochar addition accelerated the increase in pile temperature and prolonged the high temperature period (&gt;55°C) for 2 days. Under biochar influence, organic matter degradation, NH4+-N conversion and NO3−-N production was accelerated, and dissolved total organic carbon (DOC) and dissolved total nitrogen (DTN) utilization by microorganisms were enhanced. Biochar addition altered the microbial community and promoted the vital activity of Actinobacteria in the later composting stage. The antibiotics removal efficiency (except danofloxacin and enrofloxacin) was accelerated in the early composting stage (1–14 days) by biochar addition, the pile temperature had a positive effect on antibiotics removal, and the total antibiotics removal efficiency in CK and CK+Biochar treatments was 69.58% and 78.67% at the end of the composting process, respectively. The absolute abundance of most of the ARGs in the CK+Biochar treatment was lower than that in the CK treatment during composting, and the ARGs removal mainly occurred in the early (1–14 days) and later (28–50 days) stages. Biochar addition reduced the absolute abundance of MGEs (intI1, intI2) in the compost product, and most of the ARGs had a significant positive correlation with MGEs. Network analysis and redundancy analysis showed that ARGs and MGEs occurred in various host bacteria (Firmicutes, Actinobacteria, Bacteroidetes, Proteobacteria, and Halanaerobiaeota), and that DTN and NH4+-N are the main factors regulating the changes in bacterial communities, antibiotics, ARGs, and MGEs during composting. Moreover, MGEs contributed the most to the variation in ARGs. In summary, biochar addition during composting accelerated antibiotics removal and inhibited accumulation and transmission of ARGs. The results of this study could provide theoretical and technical support for biochar application for antibiotics and ARGs removal during livestock and poultry manure composting.
Effect of expanded polytetrafluoroethylene thickness on paclitaxel release and edge stenosis in stent graft
Authors: Zhu Qing , Ye Ping , Niu Haifeng , Chang Zhaohua 
Published: July 2022

Stent grafts have been widely used to treat lower extremity arterial stenosis or occlusion. However, there are major issues with edge stenosis and loss of patency over time. Paclitaxel-coated stent grafts have been proven to be effective in preventing edge stenosis, but the insufficient amounts of paclitaxel released may limit the effectiveness of drug-eluting stent grafts. In this study, we examined whether paclitaxel-coated expanded polytetrafluoroethylene (ePTFE) stent graft thickness influences paclitaxel release properties and inhibits edge stenosis. Low-, medium-, and high-thickness paclitaxel-coated stent grafts were prepared by varying the thickness of inner and outer ePTFE layers. Surface morphologies of the stent grafts were analyzed using a scanning electron microscope. The stent grafts were then implanted in the iliac arteries of 20 healthy swine. Twelve pigs were used to assess edge stenosis, and digital subtraction angiography was performed at day 30 (n = 4), 90 (n = 4), and 180 (n = 4). Histological evaluation of the treated arteries was also performed. Eight pigs were used for pharmacokinetic analysis, and the treated arteries were obtained at day 1 (n = 2), 30 (n = 2), 90 (n = 2) and 180 (n = 2). Scanning electron microscopy confirmed that the mean pore size of the stent grafts decreased with increasing thickness. The results of angiographic and histological evaluation demonstrated that low-thickness ePTFE-stent grafts resulted in edge stenosis and apparent intimal hyperplasia at 180 days, whereas for medium-thickness ePTFE-stent grafts, no obvious edge stenosis and intimal hyperplasia was noted in the similar time period. The results of pharmacokinetic evaluation showed that at 180 days, the paclitaxel concentration of treated arteries of the medium group was 36 ± 53 ng/g, while concentrations in the low group was not detectable. Stent grafts with increased ePTFE thickness appear to allow for more delayed release of paclitaxel compared to low-thickness ePTFEs.