bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decoding the role of serum cholinesterase among type 2 diabetes mellitus patients at RIMS, Ranchi – A hospital-based study
Authors: Saha Avijit , Kumari Abha , Kumar Santosh , Kumar Abhay , Munni Chandrakanta 
Published: February 2025

ABSTRACT

Introduction:
Diabetes mellitus (DM) type 2 is a group of conditions which is defined by persistent hyperglycemia along with various metabolic disturbances in carbohydrate, protein, and lipid metabolism. This condition is characterized by variable levels of insulin resistance, impaired insulin secretion, and elevated glucose production. There are various complications like cardiovascular diseases, neuropathy, nephropathy, and retinopathy which are associated with type 2 DM (T2DM). Serum cholinesterase (ChE) is an alpha glycoprotein enzyme which is formed in the liver and associated with various chronic inflammatory conditions like diabetes, hypertension, and cardiovascular diseases. So, we want to evaluate the correlation between serum cholinesterase concentrations in T2DM and also to evaluate the utility of serum cholinesterase as a diagnostic and prognostic marker in T2DM.

Material and Methods:
A cross-sectional study was conducted involving 176 participants, divided into two groups: 88 patients diagnosed with T2DM and 88 nondiabetic individuals matched for age and sex who attended at OPD and IPD of Medicine department of RIMS, Ranchi. The height and weight are taken, BMIs are calculated, and blood pressures are recorded. Blood samples are collected from all participants to measure serum cholinesterase levels; FBS, PPBS, HbA1c, serum urea, creatinine, lipid profile, thyroid profile, liver function tests (total bilirubin, direct bilirubin, AST, ALT, alkaline phosphatase), and urine protein creatinine ratio are assessed.

Results and Discussion:
The activity of serum cholinesterase raised in T2DM compared to the nondiabetic group. The cholinesterase levels in the diabetic group revealed 9272.18 ± 3062.92, median 9661.65, and in nondiabetics, it was 3504.93 ± 1520.74, median 3267.50, with a P value of < 0.001, which is statistically significant. Our study showed a positive correlation between serum cholinesterase and HbA1c with a correlation coefficient of 0.357 and a P value of 0.001, which is statistically significant. There is also a statistically significant correlation found between serum cholinesterase and serum triglyceride and also between serum cholinesterase and very low-density lipoprotein. But in our study, we have found that there is no statistical significant correlation between serum cholinesterase and overweight (BMI ≥ 25.0). The ROC plot analysis in this study demonstrates the higher diagnostic accuracy, 89.77, of serum cholinesterase with a sensitivity of 96.59 and a specificity of 82.95.

Conclusion:
So, serum cholinesterase concentrations are significantly increased in patients with T2DM and are also associated with poor glycemic control with increased insulin resistance. These study findings demonstrate the potential of serum cholinesterase as a biomarker to monitor the disease progression and its therapeutic effectiveness for interventions in T2DM.
Prognostic markers and molecular pathways in primary colorectal cancer with a high potential of liver metastases: a systems biology approach
Authors: Bahramibanan Fatemeh , Taherkhani Amir , Najafi Rezvan , Alizadeh Neda , Ghadimipour Hamidreza , Barati Nastaran , Derakhshandeh Katayoun , Soleimani Meysam 
Published: February 2025

Background and purpose:
Colorectal cancer (CRC) holds the position of being the third most prevalent cancer and the second primary cause of cancer-related fatalities on a global scale. Approximately 65% of CRC patients survive for 5 years following diagnosis. Metastasis and recurrence frequently occur in half of CRC patients diagnosed at the late stage. This study used bioinformatics analysis to identify key signaling pathways, hub genes, transcription factors, and protein kinases involved in transforming primary CRC with liver metastasis potential. Prognostic markers in CRC were also identified.

Experimental approach:
The GSE81582 dataset was re-analyzed to identify differentially expressed genes (DEGs) in early CRC compared to non-tumoral tissues. A protein interaction network (PIN) was constructed, revealing significant modules and hub genes. Prognostic markers, transcription factors, and protein kinases were determined. Boxplot and gene set enrichment analyses were performed.

Findings/Results:
This study identified 1113 DEGs in primary CRC compared to healthy controls. PIN analysis revealed 75 hub genes and 8 significant clusters associated with early CRC. The down-regulation of SUCLG2 and KPNA2 correlated with poor prognosis. SIN3A and CDK6 played crucial roles in early CRC transformation, affecting rRNA processing pathways.

Conclusion and implications:
This study demonstrated several pathways, biological processes, and genes mediating the malignant transformation of healthy colorectal tissues to primary CRC and may help the prognosis and treatment of patients with early CRC.
A novel hybrid approach to overcome defects of CE-SELEX and cell-SELEX in developing aptamers against aspartate β-hydroxylase
Authors: Bakhtiari Hadi , Naghoosi Hamed , Sattari Sina , Vahidi Mahmoud , Khomartash Mehdi Shakouri , Faridfar Ali , Rajaeinejad Mohsen , Nikandish Mohsen 
Published: February 2025

Background and purpose:
Aptamers, a new category of molecular probes, are overthrowing antibodies in molecular diagnostics. However, there are serious problems with using aptamers for this application including poor or non-specific binding in vivo conditions. Systematic evolution of aptamers is achieved through various approaches including CE-SELEX and Cell-SELEX, each suffering its inevitable weaknesses. The shortcomings of negative selection and the lengthy procedure are Cell-SELEX’s main problems, while CE-SELEX is deprived of native targets. Here, we introduced a kind of hybrid CE-Cell-SELEX, named CEC hybrid-SELEX, for addressing these limitations in creating aptamer probes detecting human aspartate β-hydroxylase (ASPH), which is a well-established tumor biomarker, in cancer diagnostic investigations.

Experimental approach:
In our approach, the selected oligomer pool from the last cycle of CE-SELEX was sequenced and then subjected to 3 additional rounds of Cell-SELEX which provides native ASPH (CEC hybrid-SELEX). High-throughput sequencing was applied to achieve a comprehensive sight of the enriched pools. Further confirmatory investigations on oligomers with higher copy numbers were performed using flow cytometry.

Findings/Results:
Three selected oligomers, AP-CEC 1, AP-CEC 2, and AP-CEC 3, showing Kd values of 43.09 nM, 34.85 nM, and 35.92 nM, respectively, were achieved based on the affinity assessment of the ASPH-expressing cells.

Conclusion and implications:
Our research suggested that CEC hybrid-SELEX could help recognize which oligomers from CE-SELEX are more capable of binding native ASPH in vivo.
The Adverse Impact of Waterpipe Dependence on Emotions: The Obesity Factor
Authors: Alomari Mahmoud A. , Khabour Omar F. , Bani-Ahmed Ali Ahmed 
Published: February 2025

Abstract

Introduction:
Obesity and tobacco, including waterpipe smoking, are associated with diminished emotional well-being. Although concurrent obesity and waterpipe smoking consumption have been reported, the combined effect of these lifestyle components on emotional well-being is unknown. Therefore, the current study compared the positive and negative emotions in adults with waterpipe dependency and obesity.

Methods:
The study was cross-sectional (n = 691) and conducted between July 2022 and February 2023 using a convenient sampling procedure. Waterpipe dependency was measured using the Lebanese Waterpipe Dependency Scale, whereas obesity was estimated using the calculated body mass index (BMI). The Positive and Negative Affect Schedule (PANAS) was used to obtain emotions. BMI-stratified one-way ANCOVA was used for statistical analysis.

Results:
Revealed a main effect of waterpipe dependency among the overly obese individuals for both positive and negative PANAS scores (P < 0.05). Post hoc analysis showed lower positive PANAS scores in the heavy versus the none, mild, and moderate waterpipe dependency groups (P < 0.05). In addition, a greater negative PANAS score in the heavy versus none and mild groups (P < 0.01) and in the moderate versus mild (P < 0.05) dependency group. Finally, the BMI-stratified one-way ANCOVA revealed no main effect of waterpipe dependency among the rest of the BMI groups (P > 0.05).

Conclusion:
The results suggest that dependency on waterpipe smoking was associated with lower positive emotions and greater negative emotions, especially among overly obese individuals. Programs to restrain waterpipe and manage obesity are needed, especially when both are concurrently present.
Physicochemical Characterization of Gum Isolated from Sterculia setigera for Potential Application as Pharmaceutical Excipient in Tablet Formulations
Authors: Limenh Liknaw Workie , Demeke Chilot Abiyu , Birhanu Gebremariam , Selam Muluken Nigatu , Belete Anteneh 
Published: February 2025

Sterculia setigera gum, also known as gum karaya, is a vegetable gum produced as an exudate. No teratogenic and mutagenic effects were reported from gum karaya toxicity studies. The aim of this study was to extract the gum fraction of S. setigera and to characterize its properties for potential application in pharmaceutical formulations. The colour of the extracted powder was grayish white, and its loss on drying was 1.2 ± 0.7%, which showed its suitability for formulating stable tablets, especially for moisture-sensitive drugs. The total ash value of S. setigera gum was 6.98 ± 0.41%, which was within the British Pharmacopeia specification. The solubility determination of S. setigera gum showed that it was sparingly soluble in water and insoluble in organic solvents, but soluble in 1:1 hydroalcoholic mixtures of ethanol and water or methanol and water. The swelling ratio was high, which increases with temperature. Scanning electron microscope images showed irregularly shaped (near to triangular), non-spherical, and of varying sized particles. Moisture sorption increased with increased relative humidity (RH) ranging from 0.40 ± 0.32% at 20% RH to 3.13 ± 0.24% at 100% RH. The Carr’s Index (25.00 ± 0.65) and Hausner Ratio (1.33 ± 0.14) values showed passable flow properties of the gum powder. The DSC and X-ray diffraction studies showed the amorphous nature of the gum. The findings of this study showed that S. setigera gum’s physicochemical properties were within pharmacopoeial specifications and showed its potential applications as a pharmaceutical excipient such as a binder or a sustained release excipient in tablet formulations.
Atypical Antipsychotics Medication Side Effects and its Associated Factors among Patients with Schizophrenia at Amanuel Mental Specialized Hospital, Addis Ababa, Ethiopia
Authors: Beyene Melak Gedamu , Teferra Solomon , Fenta Teferi Gedif 
Published: February 2025

Schizophrenia is a severe, common mental disorder that affects around 1% of the world population. The use of atypical antipsychotics has substantially increased in recent years. This study aimed to determine the prevalence of atypical antipsychotic medication side effects and its associated factors. Cross-sectional study was conducted from 3 October 2022 to 31 August 2023. An ordinal logistic regression model was used and the analysis was done using SPSS version 25. P-value less than 0.05 was deemed statistically significant. A total of 271 patients were included for analysis. The majority of patients reported they experienced absent/ mild side effects, 200 (73.8%). The most common side effects reported were extrapyramidal side effects (136, 50.2%) and sedation and central nervous system side effects (125, 46.1%). Individuals who underwent a medication switch (adjusted odds ratio, 2.6; CI: 1.357, 5.074; p = 0.004) and substance use were experiencing higher levels of severe side effects (adjusted odds ratio, 1.9; CI: 1.027, 3.538; p = 0.041). Additionally, the odds of experiencing higher side effects among patients with comorbidity was 4.1 times higher than without comorbidity (adjusted odds ratio, 4.1; CI: 1.261, 13.496; p = 0.019). Similarly, age (adjusted odds ratio, 0.96, CI: 0.923, 0.992; p = 0.018) and total score of positive and negative syndrome scale (adjusted odds ratio, 1.01, CI: 1.003, 1.020; p = 0.011) were predictors of side effects. Overall, antipsychotic medication side effects were prevalent. The most common were extrapyramidal side effects, anticholinergic side effects, sedations, and central nervous system side effects. Clinicians should discuss treatment options and associated side effects with patients before initiating therapy and explore strategies to reduce and manage side effects.
An assessment of a possible relationship between level of frenal attachment and the severity of midline diastema among selected group of Nigerians.
Authors: OPEODU Olanrewaju Ige , TEMISANREN Orighoye Tosan , OKECHUKWU Esther Adanma 
Published: February 2025

Background: There are divergent opinions concerning the coexistence of midline diastema with high frenal attachment. While some have reported high frenal attachment in association with wide midline diastema, others have reported otherwise.
Objective: To assess the relationship between the level of frenal attachment and the severity of midline diastema
Materials and Methods: Midline diastema was measured to the nearest millimeters in a cross sectional study among one hundred and sixty-four selected group of Nigerians. The level of attachment of the median labial frenum was assessed and classified into the mucosal, gingival and the papillary attachment types. The mean width of the midline diastema was compared with the height of the frenal attachment using t-test and level of statistical significance was set at p < 0.05.
Results: One hundred and sixty-four participants consisting of 16 (9.8%) males and 148 (90.2%) females were assessed. A larger percentage (12/18) of papillary type of frenal attachment had diastema greater than 1 millimeter, while one of those with mucosal attachment had midline diastema. There was a statistically significant relationship between the presence of midline diastema and the level of frenal attachment (p<0.000). Similar findings were observed when mean width of midline diastema was compared with the frenal height (p<.000) and when mean differences of midline diastema of the various frenal heights were compared.
Conclusion: Within the limitation of this study, frenal height was found to be associated with the presence and severity of midline diastema. This may be essential in the management of midline diastema, especially in its orthodontic closure as it is considered as a malocclusion trait, which may necessitate surgical excision of the frenum.
Correlação entre desordem temporomandibular e hábitos parafuncionais em crianças na fase de dentadura mista
Authors: VIGNOLI DANIELA CARVALHO , NAKAO MARCELO , DE SOUZA BRUNO JÚLIO , FERNANDES ADNREA CARAM , DE OLIVEIRA ANTONIO GERALDO , SILVEIRA CLAYTON ALEXANDRE , VIGNOLI VINICIUS VIEIRA 
Published: February 2025

Objetivou-se neste trabalho analisar a prevalência doshábitos parafuncionais como bruxismo e sucção digital associados adisfunção temporomandibular (DTM) em crianças com dentadura mista(período intertransitório e segundo período transitório). Foramexaminadas 397 crianças das cidades de Belo Horizonte e Brumadinhocom idade entre 7 e 9 anos sem história de tratamento ortodôntico. Sinaise sintomas de DTM e hábitos como bruxismo e sucção digital foramrelatados pelos responsáveis através de questionário. Outros sinais esintomas de DTM foram detectados pelo examinador através de exameclínico intra e extra-oral. Realizou-se palpação articular e muscular, detecção de ruídos articulares e observação dos movimentos de abertura efechamento da mandíbula. Concluiu-se que a prevalência dos hábitosparafuncionais como bruxismo e sucção digital estavam presentes em35% da amostra, sendo que das crianças portadoras de hábitos 22,4%possuíam bruxismo, 7,3% possuíam o hábito de sucção digital e 5,3%possuíam os hábitos de sucção digital e bruxismo. A correlação entrehábitos parafuncionais e DTM mostrada nesta pesquisa foi baixa, sendode apenas 5%; no entanto esta correlação foi maior quando os doishábitos estavam presentes simultaneamente (14,3%).
Personal Diet, intervenção nutricional em âmbito domiciliar: relato de caso
Authors: CARNEVALLI DENISE BERTIN 
Published: February 2025

O presente estudo teve por objetivo avaliar o tratamentonutricional destinado a dois adolescentes obesos atendidos por umnutricionista especialista em Personal Diet. Através de um estudolongitudinal dos pacientes, sob orientação nutricional. Como parâmetrosde avaliação realizou-se a avaliação nutricional através do Índice deMassa Corpórea (IMC), prega subescapular (PCSE) e prega cutâneaTricipital (PCT) para a idade e verificação da quantidade de óleoutilizada na preparação, freqüência alimentar, e número de refeições dosadolescentes. Os resultados obtidos ao início do tratamentodemonstraram por meio da análise antropométrica que os doisadolescentes eram obesos. No sexo masculino, a quantidade de docesconsumidos era elevada, com uma freqüência baixa de legumes ehortaliças. Ao longo do tratamento os hábitos foram se modificando, taiscomo a diminuição do óleo utilizado na preparação. Ao final dotratamento a adolescente do sexo feminino encontrava-se estado eutróficoe o adolescente do sexo masculino encontrava-se no estado de sobrepeso.Concluindo se assim que os resultados obtidos demonstraram que otrabalho do Personal Diet contribui para a mudança de estilo de vida,levando a família a adquirir hábitos saudáveis.
Atendimento primário de vítima de projétil de arma de fogo: relato de caso
Authors: SOARES PAULO ROGÉRIO DE MEDEIROS , MORAES MARCIO RONEY EL DAHER , FAVILLA EDUARDO ESBERARD , PINTO LEONARDO AUGUSTUS PERAL FERREIRA , GANDELMANN ÍTALO HONORATO ALFREDO , CAVALCANTE MARIA APARECIDA DE ALBUQUERQUE 
Published: February 2025

As feridas por projétil de arma de fogo constituem um graveproblema de saúde pública. O Rio de Janeiro é a terceira cidade do Brasilem que mais morrem pessoas vítimas de projéteis de arma de fogo. Osferimentos por arma de fogo na face são infligidos por uma variedade dearmas, das quais as de maior incidência no Rio de Janeiro são osrevólveres e os fuzis. O tratamento dos ferimentos por projétil de arma defogo é dividido em 3 fases: inicial, intermediária e reconstrutiva. Fazemparte do tratamento inicial esforços ressuscitatórios com o objetivo deassegurar a vida do paciente. O intuito deste trabalho é relatar o casoclínico de um paciente atendido no Hospital do Andaraí, na cidade do Rio de Janeiro, vítima de projétil de arma de fogo na face, apresentando feridadilacerante, com risco de óbito pelo volume de sangue perdido, conduta etratamento realizado com finalidade de preservar a vida.
AVALIAÇÃO DA MICROINFILTRAÇÃO MARGINAL CORONÁRIA DE QUATRO MATERIAIS UTILIZADOS NA RESTAURAÇÃO PROVISÓRIA EM ENDODONTIA
Authors: DINIZ RICARDO DA SILVA , RANGEL LUIZ FELIPEL GILSON DE OLIVEIRA , GOULART RHUANA MARQUES , DA SILVEIRA JOAQUIM CARLOS FEST , NORA MÁRCIA BATITUCCI , CARDOSO CARLOS EDUARDO 
Published: February 2025

Objetivo: O objetivo do trabalho foi verificar qual material restaurador provisório apresenta melhor comportamento em relação à infiltração marginal com quatro tipos de ci-mentos provisório utilizados entre as sessões do tratamento endodôntico.Materiais e Métodos: Foram utilizados 30 molares hígidos, isentos de cáries e defeitos estruturais.Os dentes foram divididos em cinco grupos com seis dentes cada e separados da seguinte forma: grupo I - Coltosol; grupoII - Cimento Ionômero de Vidro - Vidrion®; grupo III - Bioplic; grupo IV - Villevie e grupo V - Controle. Após o preparo e colocação dos cimentos, os dentes foram imersos em solução de azul de metileno a 2% e termociclados. Após o período de 168 horas os dentes de cada grupo foram sec-cionados para avaliação. Resultados: Comparando os gru-pos entre si, não houveram diferenças estatisticamente sig-nificantes. Conclusão: Concluiu que o Bioplic® obteve o melhor selamento provisório, mas todos tiveram um grau de infiltração, mas comparando entre os outros cimentos não teve diferença estatisticamente.
Business Intelligence Adoption in Diverse Economies: A Clustering Study of Five European Countries
Authors: Ghazaryan Armênia , Khachatryan Mare , Moghadam Soomela Moosa , Tarkhanyan Lili , Hovhannisyan Davit 
Published: February 2025

Objective: This paper explores the factors that influences Business Intelligence (henceforth, BI) tool adoption and its impact on Armenian IT enterprises' performance.

Theoretical Framework: Grounded in organizational shift, technology adoption (e.g., TOE framework), and competitive advantage theories, the research employs SWOT analysis, K-Means clustering, Logistic Regression, and the Elbow method to examine competitive pressures and BI's performance impact.

Method: A mixed-methods approach was used, combining surveys of Armenian tech companies with logistic regression analysis to evaluate BI adoption's relation to performance indicators such as revenue growth, market share, and customer satisfaction. K-Means clustering compared Armenia's BI adoption levels to Lithuania, Romania, Estonia, and Denmark, based on digital infrastructure and regulatory environments.

Results and Discussion: Findings disclosed low BI adoption rates in Armenia in comparison to Estonia and Denmark. Logistic regression connected strategic planning with advanced efficiency and customer needs, while K-Means clustering grouped Armenia with Romania in lower adoption clusters. Obstacles such as the digital divide, skills gaps, and regulations were discussed.

Research Implications: The study highlights the need for Armenian policymakers to enhance digital infrastructure, foster data analysis expertise, and create favorable regulations. For businesses, it emphasizes investing in training, data governance, and aligning BI strategies with objectives.

Originality/Value: This research paper uniquely benchmarks BI adoption in Armenia against European peers, offering actionable insights for improving adoption in emerging economies and enriching BI literature
Morbimortalidad perioperatoria de las cirugías cardíacas en el Instituto Nacional Cardiovascular, Lima, Perú, durante el año 2023
Authors: Silva-Tejada Harod , Sisniegas-Razón Josué , Martínez-Ninanqui Franklin , Díaz-Chávez Zoé , Ríos-Ortega Josías C. 
Published: February 2025

Objetivo. Determinar la mortalidad posoperatoria y las complicaciones posoperatorias de pacientes sometidos a cirugía cardiaca en el Servicio de Cirugía Cardiovascular del Instituto Nacional Cardiovascular, EsSalud, Lima, Perú en el año 2023. Materiales y métodos. Estudio descriptivo, retrospectivo, de las historias clínicas de los pacientes. Resultados. En el año 2023 se realizaron 538 cirugías cardiacas en nuestro centro, la mortalidad global fue del 5,6%; sin embargo, la mortalidad de cirugías electivas fue del 4,1%. La mortalidad de la cirugía coronaria aislada fue del 1,9% y de la cirugía valvular asilada fue de 0,7%. El Stroke se presentó en el 1,5% de pacientes, la reoperación por sangrado se realizó en el 8,1% de los casos. La cirugía más frecuentemente realizada fue la valvular (ya sea aislada o multivalvular) con el 40,1% de los casos, seguida de la coronaria con el 28,6%. La estancia hospitalaria de la cirugía coronaria tuvo una mediana de 9,9 días (8-12) y de la cirugía valvular aislada fue 12,8 días (10-14). Conclusiones. Los resultados posoperatorios de la cirugía cardíaca en el Instituto Nacional Cardiovascular son aceptables y comparables a los de otros centros internacionales de alto volumen de cirugías.