bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of cytochalasin B-induced membrane vesicles isolated from IL-2 overexpression M14 melanoma cells on human peripheral blood mononuclear cells in vitro
Authors: Filin Ivan Y. , Kitaeva Kristina V. , Chulpanova Daria S. , Rizvanov Albert A. , Akhmetzyanova Elvira R. , Solovyeva Valeriya V. 
Published: July 2022

Abstract
Nowadays, immunotherapy, in particular immunotherapeutic vaccines, is a promising approach in the treatment of cancer which has already demonstrated its effectiveness. Extracellular vesicles (EVs), which are capable of delivering biologically active agents to the cells, can be a promising candidate for such vaccines. M-14 human melanoma cells were transduced with lentivirus encoding interleukin (IL)-2. Membrane vesicles from M-14 cells expressing IL-2 were isolated using cytochalasin B. The interaction of membrane vesicles with human peripheral blood mononuclear cells has been analysed. Activation of T-cells was shown, as well as a decrease in the number of NK cells, after cultivation with tumor-derived vesicles. However, no cytotoxic activity of T-cells after cultivation with tumor-derived vesicles was observed, which can be explained by their immunosuppressive properties. On the other hand, such vesicles can be a promising source of tumor-specific antigens for dendritic vaccines. Therefore, further studies are required in view of the possible use of tumor-derived vesicles as a target antigen for dendritic cells.
Spinal cord from body donors is suitable for multicolor immunofluorescence
Authors: REISSIG Lukas F. , CARRERO-ROJAS Genova , MAIERHOFER Udo , MOGHADDAM Atieh SEYEDIAN , HAINFELLNER Andreas , GESSLBAUER Bernhard , HAIDER Thomas , STREICHER Johannes , ASZMANN Oskar C. , PASTOR Angel M. , WENINGER Wolfgang J. , BLUMER Roland 
Published: July 2022

Abstract
Immunohistochemistry is a powerful tool to study neuronal tissue from humans at the molecular level. To obtain fresh neuronal tissue from human organ donors is difficult and sometimes impossible. In anatomical body donations, neuronal tissue is dedicated to research purposes and because of the easier availability, it may be an alternative source for research. In the present study, we harvested spinal cord from a single organ donor 2 hours (h) postmortem and spinal cord from body donors 24, 48, and 72 h postmortem and tested how long after death, valid multi-color immunofluorescence or horseradish peroxidase (HRP) immunohistochemistry is possible. We used general and specific neuronal markers as well as glial markers to visualize different neurons. Here we showed that it is possible to visualize molecularly different neuronal elements with high precision in the body donor spinal cord 24h postmortem and the quality of the image data was comparable to those from the fresh organ donor spinal cord. High contrast multicolor images of 24h spinal cords allowed accurate automated quantification of different neuronal elements in the same sample. Although there was antibody specific signal reduction over postmortem intervals, the signal quality was acceptable for most antibodies at 48h but no longer at 72h postmortem. In conclusion, our study has defined a postmortem time window of more than 24h during which valid immunohistochemical information can be obtained from body donor spinal cord. Due to the easier availability, neuronal tissue from body donors is an alternative source for basic and clinical research.
Dietary and lifestyle associations with microbiome diversity
Authors: Watson Katherine M. , Siemens MBSc Kyla N. , Anand Sudarshan , Gardner Ivy H. , Sharpton Thomas J. , Dewey MS Elizabeth N. , Martindale MD Robert , Gaulke Christopher A. , Tsikitis  
Published: July 2022

Abstract
BackgroundMicrobial dysbiosis has been closely linked with colorectal cancer development. However, data is limited regarding the relationship of the mucosal microbiome, adenomatous polyps and dietary habits. Understanding these associations may elucidate pathways for risk stratification according to diet.ResultsPatients undergoing screening colonoscopy were included in our prospective, single center study and divided into adenoma or no adenoma cohorts. Oral, fecal, and mucosal samples were obtained. Microbial DNA was extracted, and amplicon libraries generated using primers for the 16S rRNA gene V4 region. Patient and dietary information was collected. Of 104 participants, 44% presented with polyps, which were predominantly tubular adenomas (87%). Adenoma formation and multiple patient dietary and lifestyle characteristics were associated with mucosal microbiome diversity. Lifestyle factors included age, body mass index, adenoma number, and dietary consumption of red meats, processed meats, vegetables, fruit, grain, fermented foods and alcohol. ConclusionIn this study we showed associations between dietary habits, adenoma formation and the mucosal microbiome. These early findings suggest that ongoing research into diet modification may help reduce adenoma formation and subsequently the development of CRC.
Growth, abundance, and diversity of black mangrove (Avicennia germinans) pneumatophores in deforested and sand-filled mangrove forest at Eagle Island, Niger Delta Nigeria
Authors: Numbere Aroloye , Camilo Gerardo 
Published: July 2022

Abstract
Pneumatophore is a major channel of oxygen circulation in mangrove forest. It is hypothesized that soil condition, canopy cover and stagnant pool of water can influence pneumatophore growth. Pneumatophore abundance, diversity indices, microbial and chemical composition in sand-filled mangrove forest was studied. Six plots were established within a rectangular area measuring 4152.24m2 in a random block design to investigate the effect of soil conditions on pneumatophore growth. A total of 9,586 pneumatophores were physically counted and differentiated into four types namely 1-branch, 2-branch, 3-branch, and 4-branch pneumatophores. The ANOVA results shows that there is significant difference in the abundance of pneumatophore types (F3, 20 = 7.61, p < .001). The most abundant pneumatophore type is the one branch pneumatophore (n = 4747) while the least is the four branched pneumatophores. The stagnant pool site with silty and muddy soil has the most abundant pneumatophore growth whereas plots in the seashore site with sandy soil has the least abundant pneumatophores. In contrast, the seashore site has the highest diversity (H = 1.367) while the stagnant pool has the lowest diversity (H = 0.956). Metal concentration was higher in the stagnant pool site while microbial count is higher in the seashore site. The study shows that soil condition, canopy cover, stagnant pool plus tidal action influenced pneumatophore growth.
Learning Attentive Attribute-Aware Node Embeddings in Dynamic Environments
Authors: Ahmed Nourhan , Rashed Ahmed , Schmidt-Thieme Lars 
Published: July 2022

Abstract
Learning node embeddings is fundamentally essential for numerous applications , such as link prediction and node classification. The purpose of node embedding techniques is to learn a low-dimensional representation for each node in the graph. Many existing node representation learning methods for dynamic attributed graphs focus on preserving the temporal proximity of the nodes with relatively shallow models. However, real-life graphs are complex, and usually exhibit evolutionary patterns of node attributes and graph structure. Therefore, the current state-of-the-art models fail to capture the information in the dynamic attributed graphs and settle for sub-optimal results. In this paper, we propose a novel model for embedding nodes in dynamic attributed graphs that captures the full extent of all relevant node information and the graph interrelations as well as graph evolutionary patterns. During model training, attribute-aware node embedding is learned using both graph and node properties in a dynamic context. Experiments demonstrate that our proposed method is superior to the state-of-the-art models in link prediction tasks. In addition, it introduces a novel way for learning richer representations by fully exploiting node attributes, graph structure, and evolutionary patterns in dynamic attributed graphs.
An Efficient photocatalyst based on Polyaniline Decorated MWCNT/CeO2 for degradation of Methylene Blue (MB) Dye
Authors: Ali Abdu Hussen 
Published: July 2022

Abstract
This industrial waste contain organic dye was dropped without previous treatment which result a serious environmental pollution. Among many organic dye, methylene blue (MB) dye is an aromatic heterocyclic chemical compound and is harmful if swallowed or inhaled and also causes skin and eye irritation on contact. This study aims the degradation of this harmful dye by unique PANI-MWCNT/CeO2 nanocomposite. The PANI decorated binary nanocomposite was synthesized by polymerization of aniline on the surface of MWCNT/CeO2. The optical, structures, surface morphology and functional groups of the developed composites were studied using UV–Vis diffuse reflectance, powdered X-ray diffraction (XRD), SEM and FT-IR spectroscopic techniques respectively. The synthesized nanocomposite shows effective degradation of MB. This is due to unique properties of PANI which is the delocalized π-conjugated structures is beneficial to rapid charge separation, thus the separation efficiency of photogenerated electron (e-)– hole (h+) pairs. The operational parameter is optimized, such as effects of pH, initial dye concentration, effect of irradiation time, effect of light and catalyst load were investigated using photocatalytic degradation of PANI-MWCNT/CeO2. The results obtained indicated the efficient degradation at pH= 8 which extents to 92.5%, at 10 ppm extent to 90.93 %, at 35 min interval extent to 95.01 %, MB with PANI-MWCNT/CeO2 on light extent to 89.52 % and at 0.12 g/L extent to 97.48 % respectively. Moreover, the mechanism of scavenger reaction was performed and it confirm the most important species in the degradation process would be (-*O2) and (*OH). The recyclability of PANI-MWCNT/CeO2 for photocatalytic degradation MB was also evaluated for three consecutive cycles at optimum condition (10 ppm MB, 0.12 g/L of catalyst, at pH=8) and the results shows only an 14 % decrement was observed after three cyclic run. The practical application was evaluated on a real sewage sample solution collected from Kombolcha Textile Industry, which shows a good performance about 71.74 % degradation.
Real-time sensing and low-cost experimental setup for water quantity investigation in Nature-based Solutions
Authors: Gobatti Lucas , Martins José Rodolfo Scarati , Pereira Maria Cristina Santana , Leite Brenda Chaves Coelho 
Published: July 2022

Abstract
Nature-based Solutions (NbS) Regulatory Ecosystem Services are less tangible and more complex to quantify. Among these, research to quantify water regulation services provided by Blue-Green Infrastructure is especially relevant for a range of different applications. However, to reach this quantification, experimentalists usually need to measure the flow rate, which can be costly if using high-end industry standard sensors. The present article brings, thus, a low-cost experimental setup for real-time data capture and logging using the US-025 ultrasonic sensor. The proposed setup measures a weir tanks’ water level in order to indirectly estimate the flow rate. A successful pilot experiment is described, estimating the water quantity performance of a vegetated roof in comparison to a ceramic tiled roof. For a same event, flow rate measures taken by the proposed setup are compared to readings from a rain gauge and results show a close trend. The performance of the vegetated roof has shown substantial rainfall retention and detention when compared to the ceramic roof. It is concluded that the setup is a cost-effective tool that can be attached to inlets and outlets of different NbS for characterising a range of water flow rates capable of supporting laboratory and field data capture.
Filter media for basin infiltration: a case study
Authors: Hägg Kristofer , Pott Britt-Marie 
Published: July 2022

Abstract
This study presents a viable filter media for infiltration basins used by managed aquifer recharge (MAR) plants. Filter media properties for sand filters, such as rapid and slow sand filters, are well established. This article investigates suitable filter media based on operational experience and filter media recommendations for sand filters, which is applied to the Vomb water treatment plant (WTP), a MAR plant in south Sweden. The results from this case study showed that a filter media with an effective grain size (d10) of 0.22–0.38 mm was likely viable based on the grain sizes of the natural glacifluvial deposits. The recommendation also included an optimal d10 range of 0.22–0.30 mm to increase the retention of particles in the filter media, rather than particles clogging and reducing the permeability of the aquifer. As a result of using filter media with lower d10-values than the glacifluvial deposits, unsaturated conditions under the infiltration basins will also likely improve. Furthermore, the recommendations presented in this study include limits for certain natural occurring contaminants (such as arsenic and mercury), other grain size grading characteristics and mineral composition.
Legal Inequality of the Subjects of the Russian Federation and the Problems of Ensuring the Unity of the Legal Space
Authors: Andreеchev I. S. 
Published: July 2022

Simultaneously with the evolution of the modern Russian state, federal relations are also developing. Since 2000, one of the priorities of state development has been to ensure the unity of the Russian state. This task was realized through the formation of a unified legal system, ensuring the unity of the system of public authority, the unity of the status of the individual throughout the state, the sovereignty and state integrity of the Russian Federation, the supremacy of the Constitution of Russia and federal laws throughout its territory, the unity of the economic space. The concept of «unity of legal space» has steadily entered the constitutional and legal matter, the study of which is devoted to quite a few theoretical studies. No less than practical studies reflecting the results of the ongoing work to ensure the unity of the legal space in Russia. It is impossible to carry out such a policy without ensuring genuine legal equality of the regions that make up the Russian Federation. The diverse and numerous composition of the subjects of the Russian Federation, the diversity of their interests, factors of law-making and law enforcement practice have given rise to a multidimensional phenomenon consisting in the presence of different rights (opportunities) of the subjects of the Russian Federation implemented in the field of law-making (in the presence of uniformly established their subjects of competence and powers), to which the federal center reacts differently. The existence of such a practice indicates a violation of the principle of equality of the subjects of the Russian Federation among themselves (Part 1 of art. 5 of the Constitution of Russia) and in relations with federal state authorities (Part 5 of Article 5 of the Constitution of Russia). Such a view (approach) in research on the legal equality of the subjects of the Russian Federation and ensuring the unity of the legal space is innovative. The article reveals the depth of differences, the reasons and motives for the occurrence of such examples, and also suggests a mechanism for their settlement, using numerous practical examples, including based on the author’s experience.
Potential to Promote the Initiative “One Belt – One Way” in Russia
Authors: Xing W. , Sibirskaya E. V. , Oveshnikova L. V. 
Published: July 2022

Today, there is a fairly good level of cooperation and interaction in various spheres and on various issues between Russia and China. To a greater extent, this applies to trade and economic, military-political, cultural and educational partnerships. There are quite a lot of factors contributing to this trend, but the key ones are: the presence of a high level of military-political, economic and socio-social sovereignty, as well as stability in both countries; a good level of diplomatic missions; the range of problems and dangers of the external environment are mutually mediated and identical for both Russia and China; the foreign policy courses of the countries have many points of contact in terms of vision of global transformations and transformations of the world market, and their common vectors and promising trends coincide; there are many mutually beneficial projects in the field of economics, the implementation of which depends on the level of achievement of strategic the goals of both Russia and China both in the medium and long term. It is important to emphasize that these trends have intensified, strengthened and acquired a special conceptual meaning against the background of heightened military and political tensions in the world, the application of large-scale sectoral sanctions against Russia by many countries of the world, mostly by the United States and European countries, the strengthening of diplomatic confrontation between China and the United States, as well as in trade andin the economic sphere and on issues of determining the status of fr. Taiwan. The relevance of studying tactical and strategic initiative decisions, as well as prospects within the framework of the international model of cooperation – “One belt – one road” certainly has a high level of significance, has an applied meaning and clearly expressed socio-economic and political expediency. It is important that this initiative becomes a platform for productive and mutually beneficial cooperation for countries and various organizations with the achievement of maximum synergetic effect. This project should become the starting point for the implementation of the strategic vector in Russia’s international policy – «turn to the East». The study places clear emphasis on the promising possibility of joint implementation of this project model of international cooperation.
Effect of Firing Temperature on Density, Porosity, Impact Strength, and Macro Structure of Crucible Materials Made from Graphite, Kaolin, and Castable Cement
Authors: Latif Abdul , Rusiyanto Rusiyanto , Sunyoto Sunyoto , Kriswanto Kriswanto , Fitriyana Deni Fajar 
Published: July 2022

<p class="02abstracttext">Along with the advancement of modern times and technology, the metal sector plays a critical role in sustaining present growth. The industry's increased competitiveness has hampered the development of numerous small-scale metal casting industries. A crucible made of local graphite can only be used not more than ten melting cycles. This research aims to examine the influence of firing temperature on the density, porosity, impact strength, and macrostructure of graphite, kaolin and castable cement crucible materials. This research applies experiment method at various firing temperature, i.e. 850 °C, 900 °C, 950 °C, 1000 °C and 1050 °C. The crucible materials are made of graphite, kaolin, and castable cement. The firing process employs a heating rate of 5 °C/min and a holding time 2 hours. The results show that firing temperature influences the density, porosity, impact strength and macrostructure of crucible. The highest density at 1.86 grams/cm<sup>3 </sup>was obtained at the firing temperature of 1050 °C. The lowest porosity value of 41% was attained at firing temperature of 1050 °C. However, this value was still below the crucible index criterion. The highest impact strength of 0.003249 Joule/mm<sup>2</sup> occurred for firing temperature of 1050 °C.</p>
The influence of social media of Universitas Negeri Yogyakarta Gunungkidul Campus to create brand image on student decision making
Authors: Aulia Anugerah Tesa , Satyawan Ignatius Agung , Naini Albert Muhammad Isrun 
Published: July 2022

In 2020, Universitas Negeri Yogyakarta (UNY) opened a new vocational campus in Gunungkidul Regency. The Socialization of UNY Gunungkidul campus by the admission department cannot be performed face-to-face due to the Covid-19 pandemic. Therefore, UNY Gunungkidul Campus socializes the enrollment programs via online mode. The opening of the campus aims to equalize and improve the quality of high school education in Yogyakarta. Gunungkidul is the regency with the second highest number of poor people in Yogyakarta. UNY Gunungkidul Campus utilizes social media as a promotional media to support the formation of a brand image for student decision making. This study employed a descriptive quantitative approach using a Likert scale measurement. The population in the study was 502 students. The samples were calculated using the Albert Harkin Colton table formulation which obtained 90 respondents. This analysis utilized SPSS 16 software. The results of this study show that 64.5% of 2020 student decision making is influenced by social media, although the promotion is still centered on the UNY Official account.
Comparison of the Effects of Movement-Based Interventions Delivered Face-to-face Versus Via Telehealth on Restricted and Repetitive Behaviors of Children With Autism Spectrum Disorder
Authors: Khade Avadhut , Shuguli Megan , Su Wan-Chun , Cleffi Corina , Bhat Anjana , Srinivasan Sudha 
Published: July 2022

Background
Autism spectrum disorder (ASD) is the most common pediatric neurodevelopmental disorder with a prevalence of 1 in every 44 children. Children with ASD exhibit social communication and behavioral impairments including poor verbal and nonverbal communication and heightened frequencies of repetitive and maladaptive behaviors (RBs).

Objective
Our randomized controlled trial compares face-to-face (F2F) versus telehealth-based intervention delivery for three types of interventions: two whole-body gross motor interventions and a conventional seated play (SP) intervention on RBs in children with ASD.

Methods
A total of 45 children with a confirmed diagnosis of ASD (aged 5-14 years) were recruited, matched on age bands and level of functioning, and randomly assigned to 1 of the 3 intervention groups, creative movement (CM), general movement (GM), or SP. Sessions were 1 to 1.5 hours long and were conducted with the child twice a week over 8 weeks by an expert clinician, adult confederate, and the caregiver. We coded videos of early and late training sessions for 3 types of RBs: sensory, stereotyped, and negative. Frequencies per standard time were calculated for RBs across early and late sessions.

Results
Data from a subset of 39 children suggest that there was a significant reduction in total RBs from an early to a late session in the CM group (early: mean 34.8, SE 5.0; late: mean 24.7, SE 3.9; P=.01) but not in the GM (early: mean 34.8, SE 5.0; late: mean 24.7, SE 3.9; P=.01) and SP groups (early: mean 14.6, SE 3.1; late: mean 15.2, SE 4.0; P=.79). The reduction in total RBs in the CM group did not differ significantly between children seen F2F (mean 14.2, SE 4.4) versus via telehealth (mean 5.3, SE 3.8; P=.20). The CM group specifically reduced frequencies of sensory (early: mean 10.7, SE 1.8; late: mean 6.2, SE 1.9; P=.03) and negative (early: mean 10.7, SE 2.6; late: mean 7.4, SE 2.3; P=.01) behaviors. Similar to the trends reported above, the reductions were not significantly different for children seen F2F and via telehealth (sensory F2F: mean 5.1, SE 1.8; sensory telehealth: mean 4.7, SE 3.7; P=.92; negative F2F: mean 2.6, SE 1.3; negative telehealth mean 3.7, SE 2.1; P=.66).

Conclusions
CM activities involving music and movement are novel contexts and lead to higher frequencies of RBs at baseline compared to conventional SP activities in children with ASD. However, training children reduced the frequencies of RBs, especially negative and sensory behaviors. No improvements were found in the GM or SP groups. Although our sample size was limited, preliminary data suggests similar trends for improvement in children seen F2F versus via telehealth. Telehealth-based training seems to be a viable mode of intervention delivery for families with difficulties accessing in-person care.

Conflicts of Interest
None declared.