bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome analysis uncovers an inverse correlation between alterations in P21‐activated kinases and patient survival across multiple cancer types
Authors: Vo Jessie M. , La Linh M. , Anderson Ananda V. , Alanazi Abdulaziz H. , Somanath Payaningal R. 
Published: January 2025

AbstractCancer is a complex disease with profound societal and economic impacts, especially in metastatic cases where treatment challenges arise due to the absence of reliable biomarkers and effective therapies. While P21‐activated kinases (PAKs) play a key role in cancer progression, their potential as predictive markers for metastasis and therapeutic targets has not been fully explored. We hypothesized that genetic alterations in PAK isoforms could be linked to reduced overall patient survival. To investigate this, we used data from the cBioPortal for Cancer Genomics, analyzing several randomized, multicentered phase‐3 clinical trial datasets. The analysis revealed significant genetic alterations in PAK genes, particularly in cancers such as breast, prostate, pancreatic, and lung. Notably, elevated PAK expression was associated with poorer survival outcomes in prostate and breast cancer patients. In pancreatic and lung cancers, although a trend of poorer survival with PAK alterations was observed, it was not statistically significant. Our findings underscore the importance of PAK isoforms as potential biomarkers and therapeutic targets, particularly in metastatic cancers. Further research could lead to improved patient outcomes through targeted interventions aimed at PAK‐related pathways, with PAK serving as a reliable biomarker for the precise diagnosis, monitoring, and personalization of treatment strategies.
Analytical characteristics and performance of a new hs-cTnI method: a multicenter-study
Authors: Fasano Tommaso , Fortunato Antonio , Giacomini Greta , Aimo Alberto , Moretti Marco , Viola Valentina , Sabbatinelli Jacopo , Farneti Giorgia , Maltoni Paolo , Biguzzi Rino , Sambri Vittorio , Di Marco Nadia , Ripoli Andrea , Clerico Aldo 
Published: January 2025

Abstract

Objectives
The present multicenter study was designed to evaluate the analytical performance and the 99th percentile value of the reference healthy population i.e., 99th percentile upper reference limit of the MAGLUMI® CLIA high-sensitivity cardiac troponin I (hs-cTnI) method.

Methods
Analytical performances and the 99th percentile URL value of the chemi-luminescent-immuno-assay (CLIA) method were evaluated using validated and standardized experimental protocols. Two cohorts including healthy adult individuals were enrolled. The first one included 989 blood donor volunteers (489 women and 500 men) aged 18–70 years (mean age 43 years, interquartile range 31–54 years). The second population included 47 healthy individuals (31 women and 16 men, mean age 78 years, interquartile range 73–81 years) aged≥71 years.

Results
The distributions of hs-cTnI levels in both sexes are highly right-skewed, and men show significantly (p=0.0028) higher biomarker values than women. Moreover, in both sexes the hs-cTnI levels progressively increase after the 55 years. In the multivariate analysis (n=958), hs-cTnI was found to be significantly associated to NT-proBNP (p<0.0001), sex (p<0.0001) and BMI (p=0.0424). The 99th percentile URL values, calculated using the bootstrap method in the total reference heathy population (age≥18 years), were: Females (n=521): 5.93 ng/L (CI 95 % 5.29–8.48), Males (n=516): 9.79 ng/L (CI 95 % 6.37–17.41 ng/L), Total Population (n=1,037): 7.18 ng/L (CI 6.08–12.20 ng/L).

Conclusions
The MAGLUMI CLIA method met all the criteria for an hs-cTnI assay recommended by international guidelines. The hs-cTnI values measured with the CLIA method are higher in men compared to women at the same age, and also progressively increase after the age>55 years.
Digital writing, recreated orality, and identity: domestication and exoticization of multilingual speech on Chinese social media
Authors: Zhou Feifei , Liao Chuanlin , Yang Liu 
Published: January 2025

Abstract
With their access to an unprecedented amount of streamed multimodal, multilingual content, it becomes increasingly common among Chinese social media users to translate and transcribe multilingual speech via the digital writing function of bullet comments (superimposed writing on a moving screen played simultaneously with the video). In this article, we look at “Konger” (“intentional mishearing”) to offer a timely discussion of amateur writing/literacy practice afforded by digital technologies. Leveraging unique affordances of Chinese characters (unlike alphabetic writing), when transcribing multilingual speech (from foreign speakers and Chinese dialect speakers) users deliberately select characters that deviate from their “original” meanings, often in unexpected, humorous and ludicrous ways. Adopting digital ethnography, thematic and textual analysis, we offer three case studies involving high-profile influencers who speak non-standard Putonghua with heavy regional accents. We argue that bullet comment users’ collectively produced and intricately orchestrated Konger writings recreate new layers of orality and serve diverse identity-making purposes within an increasingly commercialized Chinese social mediascape.
Crystal structure of catena-poly[μ3-iodido-(4-bromopyridine-κ
1
N)copper(I)], C5H4BrNCuI
Authors: Liu Qingjia , Wang Litong , Meng Fanchao , Han Quanxiang , Xu Bingyu , Zheng Xiaoxin 
Published: January 2025

Abstract
C5H4BrNCuI, orthorhombic, Pna21 (no. 33), a = 11.3879(4) Å, b = 16.3292(5) Å, c = 4.2274(1) Å, V = 786.11(4) Å3, Z = 4, R

gt
(F) = 0.0304, wR

ref
(F
2) = 0.0579, T = 273(2) K.
Pursuing a Career in Rehabilitation Counseling: Insights From Student Veterans in Rehabilitation Counselor Education Programs
Authors: Granger Teresa Ann , Degeneffe Charles Edmund , Umucu Emre , Kriofske Mainella Alexandra 
Published: January 2025

Purpose:This qualitative study examined the motivations of student veterans to pursue a master’s degree in rehabilitation counseling (RC), the reasons veterans chose RC over other helping professions, and the career plans of student veterans using a master’s degree in RC.Method:Given the unique perspective of the participants, the study utilizes a phenomenological approach. Data were collected via an online survey from 33 student veterans enrolled in master’s level rehabilitation counseling education programs across the United States.Results:Motivations to pursue an RC career included wanting to help fellow veterans, help others, and address other career-focused factors. Participants chose RC over other helping professions because of the profession’s focus on disability or vocational rehabilitation. Participants described the choice as a vocational calling, a chance to work in a more desired work environment, and a response to effective program recruitment. Career plans included having a veteran-focused career, working with people with disabilities, pursuing nontraditional rehabilitation services, and being open to multiple career prospects.Conclusion:The RC profession appeals to student veterans for multiple reasons. Most participants possessed clear motivations and a career plan, in part, related to wanting to give back and assist their fellow veterans.
The Potentials of Community of Practice for Disability-Inclusive Development: A Systematic Review
Authors: Tchiaze Tsangue (Agho) Glory , Elbers Willem , Koch Dirk-Jan 
Published: January 2025

Purpose:This systematic review explores how Communities of Practice (CoPs) can contribute to disability-inclusive development in Sub-Saharan Africa and under what conditions they are effective. It also seeks to identify the gaps and challenges in the existing literature on this topic.Methodology:Adopting the PRISMA framework (Preferred Reporting Items for Systematic Review and Meta-Analysis), 4503 studies were considered. After applying predefined inclusion and exclusion criteria, 20 studies were selected for analysis.Results:The analysis reveals that CoPs use a variety of strategies to address disability challenges. Drawing upon inputs such as time, commitment, competencies, and funds, CoPs enable knowledge exchange, training sessions, learning, and research that promote inclusion outcomes in education, health, and social inclusion domains. Both internal and external conditions influence the effectiveness of CoPs, including factors such as resources, leadership support, and community engagement.Conclusion:The review underscores the potential of CoPs in addressing disability challenges in Sub-Saharan Africa, demonstrated by short-term outputs and medium- to long-term outcomes. It also finds that CoPs in developing countries face unique challenges, such as power disruptions, limited internet access, and financial constraints, which are less prominent in other settings. This review calls for more diverse and comprehensive research on disability CoPs in Sub-Sahara Africa, focusing on intersectionality and extended-term effects.
Kajian Ekokritik dalam Novel Kisah Seekor Camar dan Kucing yang Mengajarinya Terbang Karya Luis Sepulveda
Authors: Vita Diah Setyoningrum  , Mustaqim Akhmad , Muslimah Adisha Nur Izzatil , Septian Mohammad Imam Ghozali Fajar 
Published: January 2025

Penelitian ini bertujuan untuk membahas ekokritik dalam novel Kisah Seekor Camar dan Kucing yang Mengajarinya Terbang. Fokus dalam penelitian ini adalah perilaku manusia terhadap lingkungan, dampak perilaku manusia terhadap alam, dan bentuk kepedulian lingkungan yang diangkat oleh pengarang. Metode yang digunakan adalah kualitatif dekriptif. Tahap pengumpulan data dalam penelitian ini, yaitu 1) membaca sumber data secara berulang; 2) mencatat data berdasarkan fokus penelitian; 3) data yang sudah didapat, lalu dipilah sesuai kebutuhan dalam penelitian; 4) data dianalisis menggunakan teori ekokritik; dan 5) membuat kesimpulan. Hasil penelitian menunjukkan bahwa perilaku manusia yang dianggap remeh telah merugikan makhluk lainnya dan menimbulkan dampak bagi lingkungan sekitar. Akan tetapi, pengarang juga memberikan solusi melalui bentuk kepedulian yang dijabarkan.
Current State and Prospects for Improving Metrological Support in the Field of Measuring the Thickness of Coatings Using the X-ray Fluorescence Method
Authors: Shipitsyna M. V. , Tyurnina A. E. 
Published: January 2025

The authors were prompted to discuss the practice of using the X-ray fluorescence for measuring coating thickness in the scientific community by the high demand for the method, which is constantly growing in the Russian Federation as the high-tech sector of mechanical engineering, instrument making and electronics develops in the country.The review presented in the article can predetermine the prospects for improving the metrological support of the X-ray fluorescence spectrometry and, more broadly, in the field of non-destructive measurements of coating thickness in general. The authors collected and systematized bibliographic material on types of coatings, methods of their application, advantages and limitations of non-destructive measurements of coating thickness. Based on these data, questions are formulated that can provide direction for scientific research aimed at developing non-destructive methods for testing coating thickness.The published material is addressed primarily to metrologists providing control in this area and manufacturers of instruments and means for monitoring the quality of coatings. The authors allow the development of the discussion taking into account the opinion and experience of using non-destructive testing of coating thickness in the conditions of the current production.
Development of Reference Materials of Benzoic and Sorbic Acids
Authors: Krasheninina M. P. , Shokhina O. S. , Makarova S. G. , Golynets O. S. , Sergeeva A. S. 
Published: January 2025

The article introduces the development of reference materials of benzoic and sorbic acids with a certified value of the mass fraction of the main substance. The development of reference materials was carried out in several stages: identification, characterization, study of homogeneity and stability of reference materials. Identification of candidate materials for reference materials was carried out by IR Fourier spectroscopy and determination of melting point by differential thermal analysis. The candidate materials for reference materials were characterized by the indirect method «one hundred minus the sum of impurities» with confirmation of the measurement results by the direct method – the acid-base titration method. It was established that the measurement results of the mass fraction of the main substance of benzoic and sorbic acids obtained by the acid-base titration method and the mass balance method are consistent with each other taking into account the values of expanded uncertainties. The work was carried out using primary and secondary standards. The accuracy of the measurement results of the mass fraction of the main substance was increased by searching for and selecting optimal measurement modes when determining each type of impurity. The homogeneity of materials was determined using the one-way analysis of variance method; stability was determined using the regression analysis method.The developed reference materials of benzoic and sorbic acids were included in the Federal Information Fund for Ensuring the Uniformity of Measurements as GSO 12297–2023 and GSO 12298–2023. The certified value of the mass fraction of the main substance is in the range from 95.00 to 100.00 %, the limits of the permissible values of the absolute error at P = 0.95 are ± 0.5 %.The use of GSO 12297–2023 and GSO 12298–2023 for certification and control of the accuracy of methods, establishment and control of the stability of calibration characteristics of measuring instruments, verification, calibration of measuring instruments can improve the quality of food products, food raw materials and pharmaceuticals.
Preoperative and postoperative factors causing the erectile dysfunction after bladder neck and nerve sparing robot-assisted laparoscopic radical prostatectomy
Authors: Sarikaya Selcuk , Ebiloglu Turgay , Buyantemur Mehmet , Inal Halil , Bedir Selahattin 
Published: January 2025

Abstract

Prostate cancer (PCa) is a prevalent disease with good prognosis and approximately 100% disease-specific survival rate is observed for the patients with localized cancer that treated with radical prostatectomy. According to the treatment options, complications may also be seen and the most common complications are urinary incontinence and erectile dysfunction. In our study, preoperative and postoperative factors affecting erectile functions after bladder neck and nerve-sparing robot assisted laparoscopic radical prostatectomy. Between October 2016 and October 2023, a total of 500 patients had bladder neck and nerve sparing robotic radical prostatectomy. Preoperative and postoperative factors related with the erectile functions have been evaluated in detail. Before operations; age, comorbidity status (cigarette smoking, hypertension, diabetes mellitus, additional illnesses), total PSA, free PSA, free/total PSA ratio, RDE findings, Prostate MRI findings, PSA density using the MRI, CFPB results, staging results were also noted. After operations; pathology results, control PSA results, need for additional treatments, continuing control PSA results after the additional treatments were noted. The age, lymphovascular invasion at CFPB, perineural invasion and surgical margin positiveness at postoperative pathology were detected to be important possible factors for the cause of postoperative ED. There was also significant correlation between the surgical margin positiveness and perineural invasion at the postoperative pathology reports (p = 0.001) showing the neural invasion of the tumor.Postoperative ED is an important factor that must be considered in both preoperative and postoperative period. Factors associated with postoperative ED must be evaluated in detail in order to avoid ED and also postoperative ED must be treated with effective treatment modalities that have been described in the literature.
LPS-induced extracellular AREG triggers macrophages pyroptosis through EGFR/TLR4 signaling pathway
Authors: Yuan Gang , Qiao Qudi , Jiang Aolin , Jiang Zeihui , Luo Haihua , Huang Lin , Wang Yanjie , Jiang Yong 
Published: January 2025

Abstract

Amphiregulin (AREG), as another EGF family member, is anchored to the cell surface as a transmembrane protein. In response to external stimulus, its extracellular domain can be release to extracellular matrix in a paracrine or autocrine manner. However, what it plays in septic macrophages pyroptosis remain poorly understood. The role of extracellular AREG was investigated in septic macrophages, mice as well as patients. Here, we found that AREG highly expressed in sepsis increased the expression of IL-6 protein and the expression of Caspase 1, IL-1β, Nlrp3 mRNA, resulting in macrophages pyroptosis. Mechanistically, macrophages pyroptosis was aggravated by extracellular AREG pretreatment and triggered by extracellular AREG and ATP (Adenosine 5'-triphosphate). The neutralizing antibody to AREG reduced LPS-induced EGFR activation, TLR4 expression and pyroptosis. Extracellular AREG-induced macrophages pyroptosis was decreased after applying inhibitions of EGFR and NF-κB as well as knockouts of TLR4 and Myd88. Besides, oxidative extracellular AREG promotes macrophages pyroptosis. In vivo studies reveal that extracellular AREG attenuates systemic inflammation infiltration and delays animal death in septic mouse model. Furthermore, serum AREG was associated with the immune inflammatory mediator, severity and mortality rate of septic patients, and genes of AREG-mediated pyroptosis signaling pathway were highly expressed in severe patients compared normal and general septic patients. Overall, extracellular AREG aggravated or triggered macrophages pyroptosis through EGFR/TLR4/Myd88/NF-κB signaling pathway, which provided promising treatment strategies for sepsis.
Exploring the factors associated with unmet need for family planning in settings with highest prevalence in Egypt
Authors: Oraby Doaa , Mowafy Maha , Jat Tej Ram , Amin Tarek Tawfik 
Published: January 2025

Abstract

Background: Despite efforts made to expand access to family planning (FP) services, a significant proportion of currently married women in rural Upper Egypt still has an unmet need for FP. The current study sought to understand the reasons behind the unmet need for FP in two settings with highest prevalence.
Study design: The study adopted qualitative methodology including focus group discussions with 48 women with unmet need for FP and in-depth interviews with ten service providers in Assuit and Souhag Governorates, both located in upper Egypt. Data were transcribed and the transcripts were analyzed using thematic analysis.
Results: Norms and traditions favoring many children per family especially sons result in family opposition to contraceptive use, specifically the husbands and mothers-in-law. Experiencing side effects and fear of health-related consequences of contraceptive use linked to prevalent myths stand out as potential reasons for unmet need.
Conclusion: The study highlighted the gap in communication activities to defy the myths related to contraceptives and the need to focus on counseling provided by the service providers to include proper use of FP methods, potential side effects and how to deal with them instead of discontinuing contraceptive methods or refrain using them.
Nonlinear coupled vibration and control of roll system for twenty- high rolling mill
Authors: He Dongping , Duan Ziheng , Xu Huidong , Kong Xianglan , Liu Yuanming , Wang Tao 
Published: January 2025

Abstract

Precision ultra-thin strips with high precision, corrosion resistance, excellent surface finish and other properties are widely used in micro-manufacturing, microelectronics, and other high-tech fields. The vibration characteristics of twenty-high rolling mills significantly affect the surface quality of extremely thin strips. In this study, a coupled nonlinear dynamic model of a twenty-high rolling mill with linear damping and nonlinear stiffness between the roll and strip was established. The amplitude-frequency equations of the primary, secondary, and internal resonances were obtained using the multi-scale method. The influence of different parameters on the amplitude-frequency curves was analyzed. According to Lyapunov's first approximation stability criterion, the stability of the roll system was analyzed using a moving phase plane phase trajectory diagram. A tuned mass damper was designed to control the nonlinear vibration characteristics of the twenty-high rolling mill, and a coupling dynamics model between the tuned mass damper and the roll system was established. The influences of the mass, stiffness, and damping ratios on the dynamic magnification coefficient of the roll were analyzed. To minimize the peak value of the dynamic amplification coefficient, an adaptive genetic algorithm was used to optimize the parameters of the tuned mass damper, and the optimal mass, stiffness, and damping ratios were determined. Finally, the feasibility and effectiveness of the tuned-mass damper were verified using time-domain, phase, and Poincare section diagrams, which provide an important reference values and theoretical basis for the design and analysis of the roll system for the twenty-high rolling mill.
Genetic Polymorphisms in Arsenic Metabolism and Oxidative Stress Genes Linked to Increased Cardiovascular Disease Risk in Arsenic Exposed Bangladeshi Population
Authors: Guha Partha , Nowshin Anika , Chowdhury Rahee Hasan , Alam Md. Jibran , Datta Amit , Shawon Inzamamul Ismail , Bhattacharjee Amlan , Wali Fahmida Binta , Hosain Nazmul , Maruf Mohammad Fazle , Rahman Md. Zillur , Khaleda Laila , Al-Forkan Mohammad 
Published: January 2025

Abstract

Arsenic (As) contamination in Bangladesh is a pervasive and insidious public health concern, with chronic exposure through water and food linked to a spectrum of health disorders, including cardiovascular diseases (CVD). Genetic polymorphisms in arsenic metabolism (AS3MT) and oxidative stress genes (SOD2, NOS3) can substantially impact the cardiovascular consequences of arsenic toxicity in humans. In our current study, we wanted to examine whether cardiovascular diseases were associated with arsenic exposure and single nucleotide polymorphisms (SNPs) of AS3MT, SOD2 and NOS3 genes in Bangladeshi population. A total of 43 patients who went through open heart surgery and from documented arsenic-contaminated and uncontaminated areas of Chittagong and Dhaka regions were selected for this study. We observed significantly higher (P = 0.042) nail As-concentration in patients from As-affected areas and histopathology indicated significantly greater cardiac tissue injury (P = 0.033) among these As-exposed patients compared to the unexposed ones. Our genotypic analysis showed a significant association of GA genotype of AS3MT rs10748835 (P = 0.022), CA genotype of SOD2 rs2758331 (P = 0.037), TC genotype of SOD2 rs2758334 (P = 0.026), GA genotype of NOS3 rs3918181 (P = 0.036), GA genotype (P = 0.024) and AA genotype of NOS3 rs2853792 (P = 0.018) with As-induced CVD. Additionally, haplotypic analysis showed AS3MT haplotype CGAA (P = 0.0006), SOD2 haplotype CTA (P = 0.028) and ACT (P = 0.0056) and NOS3 haplotype ACA (P = 0.0197) were significantly higher in frequency among the As-exposed patient group while, AS3MT haplotype CCAA (P = 0.03) and SOD2 haplotype CCT (P = 0.0024) and ATA (P = 0.00074) showed a significantly lower frequency among the same patient group. Our findings suggest that cardiovascular diseases development may be associated with arsenic intoxication and genetic polymorphisms of AS3MT, SOD2 and NOS3 genes in Bangladeshi population.