bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of Yap1 During Limb Regeneration Results in Defective Bone Formation in Axolotl
Authors: Bay Sadık , Öztürk Gürkan , Emekli Nesrin , Demircan Turan 
Published: June 2022

AbstractThe Hippo pathway plays an imperative role in cellular processes such as differentiation, regeneration, cell migration, organ growth, apoptosis, and cell cycle. Transcription coregulator component of Hippo pathway, YAP1, promotes transcription of genes involved in cell proliferation, migration, differentiation, and suppressing apoptosis. However, its role in epimorphic regeneration has not been fully explored. The axolotl is a well-established model organism for developmental biology and regeneration studies. By exploiting its remarkable regenerative capacity, we investigated the role of Yap1 in the early blastema stage of limb regeneration. Depleting Yap1 using gene-specific morpholinos attenuated the competence of axolotl limb regeneration evident in bone formation defects. To explore the affected downstream pathways from Yap1 down-regulation, the gene expression profile was examined by employing LC-MS/MS technology. Based on the generated data, we provided a new layer of evidence on the putative roles of increased protease inhibition and immune system activities and altered ECM composition in diminished bone formation capacity during axolotl limb regeneration upon Yap1 deficiency. We believe that new insights into the roles of the Hippo pathway in complex structure regeneration were granted in this study.
An expanded deep-branching thermophilic bacterial clade sheds light on the early evolution of bacteria
Authors: Leng Hao , Wang Yinzhao , Zhao Weishu , Sievert Stefan M. , Xiao Xiang 
Published: June 2022

AbstractThe origin of thermophilic bacteria is essential to our understanding of the early evolution of life. However, due to the lack of deep-branching culturable lineages, many controversies remain. Here, we report a novel deep-branching, sulfur-reducing, thermophilic bacterium that was isolated from a deep-sea hydrothermal vent using a newly developed cultivation strategy (“Subtraction-Suboptimal”, StS). This bacterial lineage clusters together with other major thermophilic bacterial groups on phylogenomic trees and forms a robust superphylum-level clade that represents a major, early-diverging bacterial lineage. Ancestral analyses and metabolic modeling reveal the ancestor of this lineage might be a thermophilic and mixotrophic bacteria with a preference for amino acids utilization. These findings provide evolutionary insights into the early divergence of thermophilic bacteria and their adaptive strategies on early Earth.
Nr4a1 regulates inhibitory circuit structure and function in the mouse brain
Authors: Huang Min , Pieraut Simon , Cao Jasmine , de Souza Polli Filip , Roncace Vincenzo , Shen Gloria , Maximov Anton 
Published: June 2022

AbstractCentral neurons express unique repertoires of secreted and transmembrane proteins that define their synaptic connectivity. However, how these molecular programs are regulated remains poorly understood. Our study shows that, in inhibitory GABAergic interneurons in the mouse forebrain, transcription of synaptic organizer molecules is controlled by Nr4a1, a nuclear receptor whose expression is transiently induced by sensory experience and required for normal learning. Nr4a1 exserts opposite effects on local axonal wiring of Parvalbumin- and Somatostatin-positive interneurons that innervate different subcellular domains of their postsynaptic partners. Loss of Nr4a1 activity in these interneurons leads to cell-type-specific transcriptional switches in multiple gene families, including those involved in surface adhesion and repulsion. Our findings reveal a mechanism by which inducible transcription factors dynamically alter the combinatorial synaptic organizing codes for structural plasticity.
Nuclear receptor interaction protein (NRIP) as a novel actin-binding protein involved in invadosome formation for myoblast fusion
Authors: Chen Hsin-Hsiung , Han Ya-Ju , Wu Tung-Chien , Yen Won-Shin , Lai Tzu-Yun , Wei Po-Han , Tsai Li-Kai , Lai Hsing-Jung , Tsao Yeou-Ping , Chen Show-Li 
Published: June 2022

AbstractTo investigate the role of nuclear receptor interaction protein (NRIP) in myoblast fusion, both the primary myoblasts from muscle-specific NRIP-knockout mice and NRIP-null C2C12 cells (KO19 cells) exhibited a significant deficit in the fusion index during myogenesis; on the other hand, overexpressed NRIP in KO19 cells could rescue myotube formation. Furthermore, NRIP was found to interact with actin directly and reciprocally that is an invadosome component for myoblast fusion. Endogenous NRIP colocalized with components of invadosome such as F-actin, Tks5, and cortactin at the tips of cells during C2C12 differentiation, and exogenous NRIP was enriched with actin at the tip of attacking cells during myogenic fusion, implying that NRIP is a novel invadosome component. Using time-lapse microscopy and cell–cell fusion assays further confirmed NRIP directly participates in cell fusion through actin. Moreover, to map the domain of NRIP–actin binding, NRIP interacted with actin either through WD40 domains directly for binding or indirectly through the IQ domain for α-actinin 2 binding with actin. NRIP with actin binding was strongly correlated with invadosome formation and myotube fusion. Collectively, NRIP acts as a novel actin-binding protein through its WD40 or the IQ to form invadosomes that trigger myoblast fusion.
A new control strategy of stopped-rotor aircraft based on manipulation model
Authors: Gao Honggang 
Published: June 2022

Purpose
The purpose of this paper is to study the control strategy of transition mode of the stopped-rotor (SR) aircraft under the condition of redundant control and complex aerodynamic characteristics.

Design/methodology/approach
This paper first proposes a transition strategy for the conversion between helicopter mode and fixed-wing mode. Then, aiming at the redundancy of the two control systems in the transition process, a control model is proposed, which greatly simplifies the control in conversion mode. Then, to facilitate the design of the control system, the Takagi-Sugeno model of the SR aircraft in transition mode is established. Finally, an explicit model tracking and tuning parameter stability augmentation control system is designed, so that the SR aircraft has a good stability during the transition process. Then, the outer loop control system of transition flight is designed.

Findings
The simulation results show that the control strategy proposed in this paper can realize the mode conversion well. It lays a solid foundation for the subsequent engineering flight test for the SR aircraft.

Originality/value
The work done in this paper provides ideas and methods for the flight control system design of SR aircraft in transition mode. The method of designing control model to solve the coordination of redundant control system is also applicable for other multimode aircraft, which provides a simple and convenient method for the multimode aircraft control.
An exploration of identity change in post-detoxification alcohol dependent individuals
Authors: Notley Caitlin , Houghton Ben , Maskrey Vivienne , Holland Richard , Lingford-Hughes Anne , Punukollu Bhaskar , Duka Theodora , Kouimtsidis Christos 
Published: June 2022

Purpose
Dependent alcohol use is a severe addictive disorder with significant enduring consequences for health and social functioning. This study aims to inductively explore the process of identity change for alcohol dependent people progressing through a “pre-habilitation” intervention, alcohol detoxification and post-detoxification recovery support.

Design/methodology/approach
Qualitative study as a part of a process evaluation situated within a UK feasibility trial of a group-based intervention in preparation for structured alcohol detoxification. Semi-structured qualitative interviews (face-to-face or telephone) collected self-reported data on experiences of treatment provision as part of the feasibility trial. Thematic analysis of transcripts and iterative categorisation of identity-related themes and concepts was conducted with verification of analysis undertaken by a second coder.

Findings
Identity change was revealed in participant narratives around the meta themes of external (social-identity) and internal (self-identity) concepts. External influences impacting social identity were key, having influenced initiation into alcohol use, influencing acceptance of the stigmatised “alcoholic” label and then being central to the treatment journey. Internal influences on self-identity also impacted on the process of identity change. In recovery, there was hope in discovering a new “normal” identity or rediscovering normality.

Originality/value
Analysis demonstrates that moving from regular alcohol use to problematic use is a journey of identity change that is influenced at the macro (cultural), meso (group) and micro (relational) social levels. Throughout the treatment journey, social influences in gaining a new non-drinker identity are key. Findings suggest a need for long-term support through treatment and community-based groups specifically to foster positive identity change that may not have been addressed previously.
Decoding revenge buying in retail: role of psychological reactance and perceived stress
Authors: Gupta Astha Sanjeev , Mukherjee Jaydeep 
Published: June 2022

PurposeCOVID-19 pandemic-related Government restrictions on the movement of people resulted in consumers moving away from retail outlets. However, sporadic instances of an unexpected surge in retail buying happened across the world immediately after the lifting of such restrictions. This uncommon phenomenon, termed revenge buying, offered an opportunity to revive retail businesses. This paper applies Reactance Theory (RCT) and Self-determination Theory (SDT) to model consumers' revenge buying intentions.Design/methodology/approachData was collected from 384 respondents in India using validated scales. The study used structural equation modelling for model testing.FindingsCOVID-19 restrictions resulted in autonomy need frustration in consumers, which induced psychological reactance and perceived stress. Psychological reactance positively impacted, while perceived stress negatively impacted revenge buying intentions. Thus, revenge buying was observed only when the psychological reactance was more than perceived stress.Research limitations/implicationsThis study, conducted in only one country with a limited convenience sample, limits the generalizability of findings.Originality/valueThis research model the psychological consequences of need frustration to explain the sporadic incidences of revenge buying in retail outlets. Further, it proposes sales recovery strategies for retailers in the immediate post-pandemic market scenarios. Retailers' strategies should focus on alleviating stress and anxiety because of health concerns, highlighting the retail buying experience to stimulate the need to visit the outlet and positioning retail buying as a potential reactance response by consumers.
Public perceptions of the effectiveness of income provision on reducing psychological distress
Authors: Bridger Emma , Nettle Daniel 
Published: June 2022

Purpose
The purpose of this paper is to understand public perceptions of the role of income for improving mental health, since public perceptions shape political decision-making. Socioeconomic determinants such as poverty cause a great deal of mental ill-health, yet it is not clear whether the general public believes this to be true. Lay understandings of health often overemphasize the roles of individual habits and medical treatments and underappreciate the importance of socioeconomic determinants.

Design/methodology/approach
UK adults (n = 622) rated effectiveness of three interventions for reducing psychological distress: medication, psychotherapy, and providing sufficient income to cover necessities via a basic income. We manipulated whether participants rated effectiveness for an identified individual vs. the population in general. Participants also indicated their support for the introduction of the basic income scheme.

Findings
Increasing income was rated highly effective for reducing psychological distress. Effectiveness ratings for income provision were as high as those for psychotherapy, and higher than those for medication. There was also an interaction with framing: in the population framing, income provision was rated more effective than either of the other two interventions. There were high levels of support for introducing a universal basic income scheme in this population.

Originality/value
UK adults anticipate that income provision would be highly effective at reducing psychological distress, as or more effective than increasing access to psychotherapy or medication. Policymakers can assume that the public will be receptive to arguments for mental health interventions that tackle broader socioeconomic determinants, especially when these are framed in population terms.
Creating alchemy within an Irish mental health service
Authors: O’ Shanahan Kevin , O’ Flynn James , McCarthy Declan , MacGabhann Liam 
Published: June 2022

Purpose
This paper aims to describe a novel community development and how the synergies arising from the interaction of diverse perspectives (including arts and health, person centredness, co-production and shared leadership) have led to a transformative initiative for individuals and a local community in the Republic of Ireland.

Design/methodology/approach
This is a descriptive case study. It includes the personal narrative of an expert by experience who is one of the co-authors. Links to short digital films are also included along with references to internal evaluation documents and published literature. Reflections from the nurses who contributed to the initiative and a university academic are also incorporated.

Findings
A space in the community built around the principles of relational practice has evolved into an alchemical space, enabling creativity, recovery and well-being. The benefits arising out of this network of social relationships have contributed to personal recovery, integration with the local community and generated social capital. This has enhanced mental health and well-being locally.

Practical implications
Statutory Irish health policy is reflective of developments in health care internationally, with an emphasis on greater delivery of care in the community. This views citizens as active partners in the maintenance of their health and well-being. This case study illustrates how this has unfolded in practice in a rural mental health community context.

Originality/value
This paper adds to the evidence base that demonstrates the potential benefits of participation in the arts to individual recovery journeys. In addition, it shows that when arts and health perspectives coalesce with the wider domain of relational practice, the synergies arising contribute positively to the health and well-being of local communities.
Marginal cost of carbon sequestration through forest afforestation of agricultural land in the southeastern United States
Authors: Obembe Oladipo S. , Hendricks Nathan P. 
Published: June 2022

AbstractOne tool to mitigate climate change is to sequester carbon through changes in land use. The purpose of this study is to analyze the cost‐effectiveness of carbon sequestration through afforestation of cropland via the Conservation Reserve Program (CRP) in the United States. We use the correlated random effects (CRE) probit model to estimate the impact of an increase in the CRP rental payments on land use transitions between cropland and forest. Our estimates are used to simulate land use change and carbon sequestration supply curves over different time horizons. Increasing the CRP rent to reflect the social cost of carbon of $154/tonne of carbon increases annual carbon sequestered by 7.42 million tonnes, 23.58 million tonnes, and 34.96 million tonnes over 1, 5, and 10‐year horizons.
Strength of cross‐sector collaborations in co‐designing an extended rural and remote nursing placement innovation: Focusing on student learning in preference to student churning
Authors: Jones Debra , Randall Sue , Williams Anna , Waters Donna , White Danielle , Haddadan Giti , Erlandsen Anita , Hanniver Jackie , Smith Rebecca , Parr Stephen 
Published: June 2022

AbstractAimTo describe the strength of a cross‐sector and multi‐university collaboration in co‐designing an extended nursing placement innovation in rural and remote Australia.ContextRegistered nurses are Australia's largest health workforce. Short‐duration placements can limit nursing student exposure to rural and remote practice, impacting student capacity to tailor and contextualise their practice, navigate complex inequities, establish a sense of belonging and consider rural practice post‐registration. Extended nursing placements have been recommended to address these challenges, but there are no guidelines governing their development and limited resources to support implementation.ApproachMethods adopted in program development included the following: (1) collaboration establishment; (2) co‐defining challenges confronting nurse education in these contexts; (3) co‐developing guiding principles; (4) co‐designing a new approach to nurse education, the Extended Nursing Placement Program (ENPP); and (5) the co‐contribution of stakeholders to program design, implementation and evaluation. Regional stakeholders include a NSW and Victorian Local Health District/Service, three Aboriginal health services and the Royal Flying Doctor Service of Australia. University participants include two metropolitan universities, a University Department of Rural Health and final‐year Bachelor of Nursing students. Program implementation in Semester 1 of 2022 with seven final‐year nursing students.ConclusionThe authors propose that the adoption of collaborative approaches can contribute to re‐framing student nurse education and the development of a rural‐ready nursing workforce. These approaches can provide regions and universities with the opportunity to avoid student churn whilst promoting the attainment of skills required to work, live and thrive in these locations.
Mindset of obligation: Conceptualization and empirical validation of a new measure of initiation and perseverance
Authors: Milam Alex C. , Rubino Cristina , Perry Sara , Alcazar Rocio , Johnson Lars U. 
Published: June 2022

AbstractWe theoretically develop and empirically validate a measure of mindset of obligation, building on the concept of the form of commitment that receives the least attention from scholars—normative commitment. We define a mindset of obligation as the pervasive tendency to initiate new endeavors and persevere through existing roles and responsibilities out of a sense of obligation. We suggest that this mindset acts as a binding agent that affects strain and withdrawal in and outside the workplace. We find that Initiation is more maladaptive, and Perseverance is more adaptive for favorable work and nonwork outcomes. Individuals with high levels of Initiation tend to commit to tasks out of a sense of obligation, with the potential to overcommit and not deliver on these commitments. Those with high levels of Perseverance may experience a sense of accomplishment from fulfilling their commitments and may be seen as responsible or reliable by others. We describe theoretical and practical implications of the mindset of obligation construct and measure, which sheds valuable insight on normative commitment, on personality traits, and other mindset constructs. These insights add understanding about the implications of the motives one has for committing to roles and responsibilities across various domains of life.
Thrombopoietin receptor agonists increase splenic regulatory T‐cell numbers in immune thrombocytopenia
Authors: Pizzi Marco , Vianello Fabrizio , Binotto Gianni , Vianelli Nicola , Carli Giuseppe , Auteri Giuseppe , Nichele Ilaria , Sbaraglia Marta , Zoletto Simone , Scarmozzino Federico , Bresciani Rita , d'Amore Fabio , Friziero Alberto , Guzzardo Vincenza , Bertozzi Irene , Famengo Barbara , d'Amore Emanuele S. G. , Sabattini Elena , Dei Tos Angelo Paolo 
Published: June 2022

SummaryThrombopoietin receptor agonists (TPO‐RA) are a valid therapy for immune thrombocytopenia (ITP), due to megakaryocyte stimulation and (poorly characterised) immune‐modulatory effects. The spleen is pivotal in the pathogenesis of ITP, yet little is known on its immune microenvironment and on effects of TPO‐RA on this organ. To address these topics, we analysed 35 spleens removed for primary refractory ITP. Pre‐splenectomy TPO‐RA administration correlated with increased splenic regulatory T cells (Tregs), type 2 T‐helper cells and histiocyte density and with reduced red pulp sinusoids. Surgical outcome was not associated with TPO‐RA administration, other pre‐splenectomy therapies and/or Treg density. In conclusion, TPO‐RA affect the splenic microenvironment, but this has no impact on splenectomy outcome.
The growth effect of trade openness on African countries: Evidence from using an instrumental variable panel smooth transition model
Authors: Kinfack Emilie , Bonga‐Bonga Lumengo 
Published: June 2022

AbstractThis paper assesses the relationship between trade openness and economic growth in Africa by accounting for the heterogeneity of African countries. In addition, the paper contributes to the literature on trade openness and economic growth nexus by applying the instrumental variable panel smooth transition regression, a methodology that accounts for nonlinearity and endogeneity in the relationship between the two variables. The results of the empirical analysis reveal that the investment ratio is a channel through which trade openness affects economic growth in the African continent. In addition, the relationship between trade openness and economic growth varies according to the degree of a country's development in Africa. The study finds a negative relationship between openness and growth in low‐income countries. Conversely, for upper‐income countries, the coefficients of trade indicators are positive and statistically significant. The results indicate that African countries are not homogeneous, especially concerning trade openness and economic growth nexus.
The intra‐arterial selective cooling infusion system: A mathematical temperature analysis and in vitro experiments for acute ischemic stroke therapy
Authors: Jiang Miaowen , Li Ming , Gao Yuan , Wu Longfei , Zhao Wenbo , Li Chuanhui , Hou Chengbei , Qi Zhengfei , Wang Kun , Zheng Shiqiang , Yin Zhichen , Wu Chuanjie , Ji Xunming 
Published: June 2022

AbstractIntroductionThe neuroprotection of acute ischemic stroke patients can be achieved by intra‐arterial selective cooling infusion using cold saline, which can decrease brain temperature without influencing the body core temperature. This approach can lead to high burdens on the heart and decreased hematocrit in the scenario of loading a high amount of liquid for longtime usage. Therefore, autologous blood is utilized as perfusate to circumvent those side effects.MethodsIn this study, a prototype instrument with an autologous blood cooling system was developed and further evaluated by a mathematical model for brain temperature estimation.ResultsHypothermia could be achieved due to the adequate cooling capacity of the prototype system, which could provide the lowest cooling temperature into the blood vessel of 10.5°C at 25 rpm (209.7 ± 0.8 ml/min). And, the core body temperature did not alter significantly (−0.7 ~ −0.2°C) after 1‐h perfusion. The cooling rate and temperature distributions of the brain were analyzed, which showed a 2°C decrease within the initial 5 min infusion by 44 ml/min and 13.7°C perfusate.ConclusionThis prototype instrument system could safely cool simulated blood in vitro and reperfuse it to the target cerebral blood vessel. This technique could promote the clinical application of an autologous blood perfusion system for stroke therapy.