bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co‐CRISPR: A valuable toolkit for mutation enrichment in the gene editing of Spodoptera frugiperda
Authors: Han Wei‐Kang , Tang Feng‐Xian , Gao Hao‐Li , Wang Yan , Yu Na , Jiang Jian‐Jun , Liu Ze‐Wen 
Published: September 2022

AbstractThe CRISPR/Cas9 system has been successfully applied in dozens of diverse species; although the screening of successful CRISPR/Cas9 editing events remains particularly laborious, especially for those that occur at relatively low frequency. Recently, a co‐CRISPR strategy was proved to enrich the desired CRISPR events. Here, the co‐CRISPR strategy was developed in the Fall armyworm, Spodoptera frugiperda, with kynurenine 3‐monooxygenase gene (kmo) as a marker. The kmo mosaics induced by single‐guide RNAs (sgRNAs)/Cas9 displayed the darker green color phenotype in larvae, compared with wild type (brown), and mosaic‐eye adults were significantly acquired from the mosaic larvae group. In the kmo knockout strain, no significant difference was observed in larval development and adult reproduction. Acetylcholinesterase 2 (ace2) and Wnt1 were selected as target genes to construct the co‐CRISPR strategy using kmo marker. By co‐injection of kmo and ace2 sgRNAs, the mutant efficiency of ace2 was significantly increased in the kmo mosaic (larvae or adults) groups. Similarly, more malformed pupae with Wnt1 mutations were observed in the darker green larvae group. Taken together, these results demonstrated that kmo was a suitable visible marker gene for the application and extension of co‐CRISPR strategy in Fall armyworm. Using darker green color in larvae or mosaic‐eye in adults from kmo knockout as a marker, the mutant efficiency of a target gene could be enriched in a Fall armyworm group consisting of marked individuals. The co‐CRISPR strategy is helpful for gene function studies by the knockout technique with no or lethal phenotypes.
Potential impacts of reduced inflows to the Salton Sea: Forecasting non‐market damages
Authors: Ayres Andrew , Adams Taylor , Carron John , Cohen Michael , Saracino Anthony 
Published: September 2022

AbstractTerminal lakes throughout the American West provide important amenity and environmental values, but many are shrinking due to reduced inflows and warming temperatures. In California's Imperial Valley, agricultural water use reductions diminish inflows supporting the Salton Sea, a terminal desert lake and important environmental amenity for both the region and the state as a whole. The costs of these reduced inflows are difficult to monetize yet complicate management decisions. We assess the costs of potential future drought‐induced transfers by linking novel hydrologic scenarios to an economic framework for quantifying local and regional damages based on existing estimates of non‐market environmental values from the literature. The costs of lost wetland ecosystems, increases in particulate matter from exposed playa, and other local disamenities are substantial. For the scenarios considered, they range between approximately $500 million and $1 billion in present value (2019 USD). Estimated damages per acre‐foot (or thousand m3) of reallocated water exceed several thousand dollars. The majority arise from loss of wetland habitat; incremental particulate matter damages are relatively modest in our modeling but exacerbate salient air quality issues in the region. Interest in reallocating water from Imperial Valley, the largest user of Colorado River water, to other applications will increase over time. Our work highlights the importance of evaluating the impacts of such efforts.
Malthus and gender
Authors: Bashford Alison 
Published: September 2022

AbstractThis article re‐reads Malthus'sEssay on the Principle of Populationfor his explicit discussion of men and women, masculinity and femininity. A feminist reading is possible, but not undertaken here. Rather, the purpose is simply to demonstrate how ‘gender’ was Malthus's own object of inquiry. Historical actors, perhaps especially economic thinkers, often considered gender far more fully and explicitly than almost all subsequent analysts of them. It therefore remains not just insufficient, but empirically erroneous not to inquire into how ‘men’ and ‘women’ were considered, constructed, instructed, symbolised or valued by the historical actors we study, including those in the political economy canon.
Truncated CSF3 receptors induce pro‐inflammatory responses in severe congenital neutropenia
Authors: Olofsen Patricia A. , Bosch Dennis A. , de Looper Hans W. J. , van Strien Paulina M. H. , Hoogenboezem Remco M. , Roovers Onno , van der Velden Vincent H. J. , Bindels Eric M. J. , De Pater Emma M. , Touw Ivo P. 
Published: September 2022

SummarySevere congenital neutropenia (SCN) patients are prone to develop myelodysplastic syndrome (MDS) or acute myeloid leukaemia (AML). Leukaemic progression of SCN is associated with the early acquisition of CSF3R mutations in haematopoietic progenitor cells (HPCs), which truncate the colony‐stimulating factor 3 receptor (CSF3R). These mutant clones may arise years before MDS/AML becomes overt. Introduction and activation of CSF3R truncation mutants in normal HPCs causes a clonally dominant myeloproliferative state in mice treated with CSF3. Paradoxically, in SCN patients receiving CSF3 therapy, clonal dominance of CSF3R mutant clones usually occurs only after the acquisition of additional mutations shortly before frank MDS or AML is diagnosed. To seek an explanation for this discrepancy, we introduced a patient‐derived CSF3R‐truncating mutation in ELANE‐SCN and HAX1‐SCN derived and control induced pluripotent stem cells and compared the CSF3 responses of HPCs generated from these lines. In contrast to CSF3R‐mutant control HPCs, CSF3R‐mutant HPCs from SCN patients do not show increased proliferation but display elevated levels of inflammatory signalling. Thus, activation of the truncated CSF3R in SCN‐HPCs does not evoke clonal outgrowth but causes a sustained pro‐inflammatory state, which has ramifications for how these CSF3R mutants contribute to the leukaemic transformation of SCN.
Molecular landscapes of longitudinal NF2/22q and non‐NF2/22q meningiomas show different life histories
Authors: Ng Ho‐Keung , Li Kay Ka‐Wai , Chung Nellie Yuk‐Fei , Chan Janice Yuen‐Tung , Poon Manix Fung‐Man , Wong Queenie Hoi‐Wing , Kwan Johnny Sheung‐Him , Poon Wai‐Sang , Chen Hong , Chan Danny Tat‐Ming , Shi Zhi‐Feng , Mao Ying 
Published: September 2022

AbstractRecurrence is a major complication of some meningiomas. Although there were many studies on biomarkers associated with higher grades or increased aggressiveness, few studies specifically examined longitudinal samples of primary meningiomas and recurrences from the same patients for molecular life history. We studied 99 primary and recurrent meningiomas from 42 patients by FISH for 22q, 1q, 1p, 3p, 5q, 6q, 10p, 10q, 14q, 18q, CDKN2A/B homozygous deletion, ALT (Alternative Lengthening of Telomere), TERT re‐arrangement, targeted sequencing and TERTp sequencing. Although NF2 mutation and 22q were well known to be aetiological events in meningiomas, we found that in these paired meningiomas, combining the two events resulted in an NF2/22q group (57 tumors from 25 patients) which were almost mutually exclusive with those cases without these two changes (42 tumors from 17 patients) for NF2/22q. No other molecular changes were totally unique to NF2/22q or non‐NF2/22q tumors. For molecular evolution, NF2/22q meningiomas had higher cytogenetic abnormalities than non‐NF2/22q meningiomas (p = 0.003). Most of the cytogenetic changes in NF2/22q meningiomas were present from the outset whereas for non‐NF2/22q meningiomas, cytogenetic events were uncommon in the primary tumors and most were acquired in recurrences. For non‐NF2/22q tumors, CDKN2A/B homozygous deletion, 1q gain, 18p loss, 3p loss, and ALT were preferentially found in recurrences. Mutations were largely conserved between primary and recurrent tumors. Phylogenetic trees showed 11/11 patients with multiple recurrent tumors had a conserved evolutionary pattern. We conclude that for molecular life history, NF2 and 22q should be regarded as a group. NF2/22q recurring meningiomas showed more cytogenetic abnormalities in the primary tumors, whereas non‐NF2/22q meningiomas showed CDKN2A/B deletion and other cytogenetic abnormalities and ALT at recurrences. Although chromosome 1p loss is a known poor prognostic marker in meningiomas, it was also associated with a shorter TBR (time between resection) in this cohort (p = 0.002).
Decoding post‐orogenic sediment recycling and dispersal using detrital zircon core and rim ages
Authors: Liu Li , Xu Jie , Stockli Daniel F. , Lawton Timothy F. , Blakey Ronald C. 
Published: September 2022

AbstractDetrital zircon (DZ) geochronology has become a popular tool in provenance studies during the past two decades. However, similar zircon crystallization ages from different source regions greatly hamper the interpretation of sediment dispersal and recycling processes. The Alleghenian–Ouachita–Marathon (AOM) foreland and vicinity in North America is a region where some dominant DZ age groups could come from both the southern Appalachians in the eastern United States and the Gondwanan terranes in Mexico. In this study, we present 1045 new DZ U–Pb ages and 81 DZ core–rim age pairs in lower Permian sandstone in the Permian Basin and Miocene sandstone in the eastern Gulf of Mexico (GOM). These new data were integrated with published DZ single U–Pb age and core–rim ages from syn‐ to post‐orogenic strata in the Permian Basin, Marathon foldbelt, southern Appalachian foreland basin and eastern GOM to interpret the sediment‐dispersal models in the AOM foreland and eastern GOM. Our models show that during the Leonardian Stage, sediments derived from the Appalachians were first delivered to the US midcontinent and then recycled to the Permian Basin; during the Miocene, sediment from the Appalachians fluxed to the eastern GOM, with no longshore mixing from the western GOM. These models based on the integration of single U–Pb and core–rim ages are consistent with published results that used other methods, including zircon single U–Pb age, zircon Hf isotopic data, zircon (U–Th)/He age, sedimentology and stratigraphy. Our results demonstrate that although some limitations exist, zircon core–rim age is a powerful tool, adding an extra constraint on the interpretation of sediment‐dispersal systems. This tool is particularly applicable to the post‐orogenic stage, during which the sediment pathways are more complicated because of the dominant input from distal sources. Insights gained in this study imply that this novel strategy of using core and rim ages could be integrated with other methods to better understand sediment dispersal.
Drug formulation augments the therapeutic response of carboplatin administered through a lymphatic drug delivery system
Authors: Mishra Radhika , Sukhbaatar Ariunbuyan , Dorai Arunkumar , Mori Shiro , Shiga Kiyoto , Kodama Tetsuya 
Published: September 2022

AbstractTreatment of metastatic lymph nodes (LNs) is challenging due to their unique architecture and biophysical traits. Systemic chemotherapy fails to impede tumor progression in LNs due to poor drug uptake and retention by LNs, resulting in fatal systemic metastasis. To effectively treat LN metastasis, achieving specific and prolonged retention of chemotherapy drugs in the tumor‐draining LNs is essential. The lymphatic drug‐delivery system (LDDS) is an ultrasound‐guided drug‐delivery methodology for administration of drugs to LNs that addresses these requirements. However, early‐stage metastatic LNs have an additional set of drug transport barriers, such as elevated intranodal pressure and viscosity, that negatively impact drug diffusion. In the present study, using formulations of elevated osmotic pressure and viscosity relative to saline, we sought to favorably alter the LN's physical environment and study its impact on pharmacokinetics and consequently the therapeutic efficacy of carboplatin delivered using the LDDS. Our study confirmed the capability of a drug formulation with elevated osmotic pressure and viscosity to alter the architecture of LNs, as it caused notable expansion of the lymphatic sinus. Additionally, the study delineated an optimal range of osmotic pressure and viscosity, centered around 1897 kPa and 11.5 mPa·s, above and below which therapeutic efficacy was found to decline markedly. These findings suggest that formulation osmotic pressure and viscosity are parameters that require critical consideration as they can both hinder and promote tumorigenesis. The facile formulation reported here has wide‐ranging applicability across cancer spectrums and is thus anticipated to be of great clinical benefit.
‘Disability is not a word we use’: Social workers' professional judgements about support for disabled young people leaving care
Authors: Bennwik Ingri‐Hanne Braenne , Oterholm Inger , Kelly Berni 
Published: September 2022

AbstractDisabled young people leaving care often experience a more complex transition to adulthood than other youths. Still, policy and services can fail to recognize the intersection between a young person's care experiences and disability. Drawing on data from a qualitative interview study with 14 social workers who work with aftercare in the Norwegian child welfare services, we investigate social workers' professional judgements about support for this subgroup of the leaving care population. Our analysis uses the theoretical construct of institutional logics and shows that social workers did not include concepts of disability in their judgements about support for these young people. Instead, the social workers' considerations were guided by three other organizing principles: a ‘medical logic’, an ‘activation logic’ and an ‘aftercare logic’. We discuss these findings in light of critical disability studies and argue for a more nuanced understanding of disability in social work practice with care leavers. Highlighting disability rights and going beyond diagnosis and categorisations of disabled people can challenge a medical model approach to service provision.
Metal ions in cerebrospinal fluid: Associations with anxiety, depression, and insomnia among cigarette smokers
Authors: Li Yuying , Wu Fenzan , Mu Qingshuang , Xu Kewei , Yang Shizhuo , Wang Ping , Wu Yuyu , Wu Junnan , Wang Wei , Li Hui , Chen Li , Wang Fan , Liu Yanlong 
Published: September 2022

AbstractObjectiveThe study aimed to investigate the relationship between cerebrospinal fluid (CSF) metal ions and anxiety, depression, and insomnia among cigarette smokers.MethodsWe measured CSF levels of various metal ions from 178 Chinese male subjects. Apart from sociodemographic and clinical characteristics data, the Fagerstrom Test for Nicotine Dependence (FTND), Beck Depression Inventory (BDI), Self‐Rating Anxiety Scale (SAS), and Pittsburgh Sleep Quality Index (PSQI) were applied.ResultsBDI and PSQI scores (all p < 0.001) were significantly higher in active smokers than nonsmokers. Active smokers have significantly higher CSF levels of magnesium, zinc, iron, lead, lithium, and aluminum (all p ≤ 0.002). Some metal ions, including zinc, iron, lead, and aluminum, were found to have a significant correlation with BDI scores, whereas metal ions, including zinc and lead, were found to have a significant correlation with PSQI scores in the general group. More interesting, mediation analysis showed that aluminum mediated the relationship between smoking and depression.ConclusionsCigarette smoking was indeed associated with depression and insomnia. Active smokers had significantly higher CSF levels of magnesium, zinc, iron, lead, lithium, and aluminum. Furthermore, CSF aluminum played a mediating role in the relationship between smoking and depression, which further confirmed its neurotoxicity.
Classic or hybrid? The performance of next generation ecological models to study the response of Southern Ocean species to changing environmental conditions
Authors: Guillaumot Charlène , Belmaker Jonathan , Buba Yehezkel , Fourcy Damien , Dubois Philippe , Danis Bruno , Le Moan Eline , Saucède Thomas 
Published: September 2022

AbstractAimIn the context of intensifying threats of climate change on marine communities, ecological models are widely applied for conservation strategies, though polar studies remain scarce given the limited number of datasets available. Correlative (e.g. species distribution models, SDM) and mechanistic (e.g. dynamic energy budget models, DEB) modelling approaches are usually used independently in studies. Using both approaches in integrative, hybrid models could help to better estimate the species potential ecological niche, as mechanistic and correlative models complement each other very well, giving more insights into species potential response to fast‐changing environmental conditions.LocationThe study focusses on the Baie du Morbihan, a silled basin located in the east of the Kerguelen Islands (sub‐Antarctic).MethodsA hybrid, correlative‐mechanistic model was implemented to predict the response of the endemic sea urchin Abatus cordatus (Verrill, 1876). We compared the performances of classic and integrated approaches to predict A. cordatus distribution according to two dates representing seasonal contrasts. Two integrated approaches were studied and performed by either (1) including the spatial projection of the DEB model as an input layer inside the SDM (‘integrated SDM‐DEB’) or (2) using a Bayesian procedure to use DEB model outputs as priors of the SDM (‘integrated Bayesian’ approach).ResultsResults show higher performances of ‘integrated Bayesian’ approaches to evaluate A. cordatus potential ecological niche compared with ‘classic’ and ‘integrated SDM‐DEB’ methods. The influence of environmental conditions on model predictions is further captured with these Bayesian procedures and better highlights the environmental influence on the species‐predicted distribution. Model performance is good for the different simulations, and uncertainty in predictions is well‐highlighted.Main conclusionsThe good performances of ‘integrated Bayesian’ approaches to estimate species potential ecological niche opens perspectives for future applications to a broad panel of natural examples, noteworthy for decision‐making and conservation management purposes.
Organ transplantation in Nepal: Ethical, legal, and practical issues
Authors: Atreya Alok , Upreti Manish , Menezes Ritesh George , Dawadi Ambika , Subedi Nuwadatta 
Published: September 2022

AbstractIn Nepal, live donor organ transplantation is only 14 years old with the first successful kidney transplant made in 2008 and a successful liver and bone marrow transplant made in 2016. However, transplantation of cadaveric cornea dates back to 1998. There are still no cases of animal‐to‐human organ transplantation in Nepal. There are stringent laws to regulate human body organ transplantation in Nepal which are amended from time to time. However, there is a racket of human traffickers who lure rural people from this low‐income country into the illegal organ trade. Furthermore, there is a substantial lack of awareness of organ donation among the general public. This article focuses on the stipulations of ethical, legal, and practical issues of obtaining organs procured from living and brain‐dead donors that support the process of transplantation in Nepal. In addition, the article also explores the legal and practical issues of organ trafficking and organ donation awareness in Nepal on the basis of factual data and findings from other studies.
Neglected symbionts and other metazoan invertebrates associated with molluscs from Africa's largest lake: Diversity, biotic interactions and bioindication
Authors: Outa James Omondi , Hörweg Christoph , Avenant‐Oldewage Annemariè , Jirsa Franz 
Published: September 2022

Abstract

Freshwater molluscs are hosts for diverse metazoan symbionts. However, apart from the digenean helminths, symbionts of molluscs are underreported worldwide. Therefore, this study focused on the diversity of oligochaetes, leeches, mites, insect larvae and nematodes associated with gastropods and bivalves from Lake Victoria, East Africa.

Overall, 1,633 mollusc specimens representing 14 species were sampled from the Kenyan part of the lake. Each host specimen was examined to determine co‐occurrence of symbionts (including digeneans) and other metazoan invertebrates, and their microhabitat preferences on/in the hosts. In addition, prevalence and abundance of symbionts were compared for molluscs obtained from an unpolluted site, and sites that are eutrophic and polluted with heavy metals.

In total, 33 species were recovered from the molluscs: six oligochaetes, four insects, two mites, two nematodes, one leech and 18 digeneans. The nematode Daubaylia potomaca, mite Unionicola macani, leech Batracobdelloides sp., oligochaetes Chaetogaster limnaei, Dero digitata, Aulophorus africanus, Allonais paraguayensis and Ophidonais serpentina, and gorgoderid digenean larvae are new records for Lake Victoria. Moreover, the genus Bratislavia (Oligochaeta) is reported for the first time in Africa. Our results show that parasitic mites and leeches were absent from mollusc specimens that harboured chironomids. Likewise, there was no co‐occurrence of C. limnaei, D. potomaca and digeneans, suggesting that antagonistic interactions occur between the symbionts. Although co‐occurrence of mites and digeneans in individual mollusc hosts was common, the symbionts occupied different microhabitats. This study shows that prevalence and intensities of U. macani and C. limnaei, were significantly higher in hosts from the unpolluted site, compared with the polluted sites. What is more, D. potomaca and trichopteran larvae, were recorded only from the unpolluted site. The distribution of the chironomid Kiefferulus chloronatus suggests their preference for sites that are rich in organic matter and their ability to tolerate pollution.

Information on antagonistic interactions between symbionts might be applicable in biocontrol, especially of digenean species that have veterinary or medical importance. Finally, this study shows a potential for the use of U. macani, C. limnaei, D. potomaca and insect larvae as discriminators of the quality of aquatic habitats.