bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biting behaviour and the spatio-temporal dynamics of malaria vectors in different ecological zones in Ghana
Authors: Akuoko Osei Kwaku , Dhikrullahi Shittu Bunkumni , Hinne Isaac Amankona , Mohammed Abdul Rahim , Owusu-Asenso Christopher Mfum , Coleman Sylvester , Dadzie Samuel Kweku , Kyeremanteng Rosina , Boakye Daniel Adjei , Wilson Michael David , Afrane Yaw Asare 
Published: August 2022

Abstract
Background: Significant decrease in malaria morbidity and mortality have been attained using long-lasting insecticide treated nets and indoor residual spraying. Selective pressure from these control methods influences change in vector bionomics and behavioural pattern. There is a need to understand how insecticide resistance drives behavioural changes within vector species. This study aimed to determine the spatio-temporal dynamics and biting behaviour of malaria vectors in different ecological zones in Ghana in an era of high insecticide use for public health vector control.Methods: Adult mosquitoes were collected during the dry and the rainy seasons in 2017 and 2018 from five study sites in Ghana in different ecological zones. Indoor and outdoor biting mosquitoes were collected per hour from 18:00 to 06:00 hours employing the human landing catches (HLC) technique. Morphological and molecular species identification of vectors were done using identification keys and PCR respectively. Genotyping of insecticide resistant markers was done using the TaqMan SNP genotyping probe-based assays. Detection of P. falciparum sporozoites was determined using PCR.Results: A total of 50,322 mosquitoes belonging to four different genera were collected from all the study sites during the sampling seasons in 2017 and 2018. Among the Anophelines were Anopheles gambiae s.l. 93.16%, (31055/33,334), An. funestus 2.07%, (690/33,334), An. pharoensis 4.63%, (1545/33,334), and An. rufipes 0.13% (44/33,334). Overall, 76.40%, (25,468/33,334) of Anopheles were collected in the rainy season and 23.60%, (7,866/33,334) in the dry season. There was a significant difference (z = 2.410; p = 0.0160) between indoor biting (51.09%; 15,866/31,055) and outdoor biting An. gambiae s.l. (48.91%; 15,189/31,055). The frequency of the Vgsc-1014F mutation was slightly higher in indoor biting mosquitoes (54.90%) than outdoors (45.10%). Overall, forty-four pools (44) of samples were positive for P. falciparum CSP giving an overall sporozoite rate of 0.07%.Conclusion: Anopheles gambiae s.l. were more abundant indoors across all ecological zones of Ghana. The frequency of G119S was higher in indoor than outdoor from all the study sites, but higher sporozoite rates in outdoor mosquitoes in Dodowa and Kpalsogu. There is thus, an urgent need for a supplementary malaria control intervention to control outdoor biting mosquitoes.
Identification of TMPRSS2 and ERG as androgen receptor targeted putative highly significant genes in PC3-AR cells from AR ChIP-Seq and ChIP-chip data
Authors: Hong Xu 
Published: August 2022

Abstract
Background:The TMPRSS2 and ERG which could form the TMPRSS2-ERG gene fusion are two important genes in prostate cancer cells.Previous works by others have found that the ERG could interrupt androgen receptor (AR) signal transducting pathway and the TMPRSS2-ERG gene fusion acts in a pivotal role in prostate cancer progression.Results: In this study, through transfecting with wild-type androgen receptor with an androgen receptor negative prostate cancer cell line(PC3), both the androgen receptor(AR) ChIP-Seq and ChIP-chip data are generated for the androgen receoptor in the advanced PC3-AR cells. After a series of bioinformatics data analysis, it is found that TMPRSS2 and ERG genes are androgen receptor targeted putative highly significant genes in androgen receptor ChIP-Seq and ChIP-chip datasets in PC3-AR cells.Conclusions: Identifying of TMPRSS2 and ERG as androgen receptor targeted putative highly significant genes in advanced PC3-AR cells could serve the international scientific community for biomarker identifications and developing novel prostate cancer therapeutic strategies.
Cardioprotective effect of aescin on antioxidant and lipid profile in isoproterenol-induced myocardial infarction in rats
Authors: Kaliyamoorthi Kanimozhi , Loordhurani Asaikumar , Subashchandrabose Olikkavi , Lakshmanan Vennila , Ganapathi Sindhu , Sathiavakoo Vigil Anbiah 
Published: August 2022

Abstract
The efficacy of aescin on the liver and cardiac markers, lipid profile, and antioxidant status in rats with myocardial infarction (MI) induced by isoproterenol (ISO) was investigated in this study. Three doses of aescin (5, 10, and 20 mg/kg of b.w) were administered to rats for the first 21 days. After the treatment period, ISO (60 mg/kg of b.w) was given subcutaneously to the rats on the 22nd and 23rdday. Cardiovascular and hepatic markers (CK, ALT, CK-MB, AST, cTnI, and cTnT) have been analyzed to investigate the cardiac and liver damage. The activities of antioxidant enzymes (CAT, GST, SOD, and GPx) were decreased in both cardiac tissue and erythrocytes of ISO rats. The levels of phospholipids (PLs), total cholesterol (TC), free fatty acids (FFA), and triglycerides (TG) were increased significantly in the serum of the rats administrated with ISO. The results of the present study implies that aescin pretreatment reduces oxidative stress and exhibits cardioprotective action by scavenging the free radicals and maintaining the levels of circulatory and cardiac lipids. Hematoxylin and eosin staining method was used to examine the cardiac histological changes in the experimental rats and the results showed that ISO-administered rats pretreated with aescin reduced cardiac tissue damage when compared with ISO alone injected rats.
Harvest control rules used in U.S. federal fisheries management and implications for climate resilience
Authors: Free Christopher M. , Mangin Tracey , Wiedenmann John , Smith Conner , McVeigh Halley , Gaines Steven D. 
Published: August 2022

Abstract
Climate change is altering the productivity of marine fisheries and challenging the effectiveness of historical fisheries management. Harvest control rules, which describe the process for determining catch limits in fisheries, represent one pathway for promoting climate resilience. In the United States, flexibility in how regional management councils specify harvest control rules has spawned diverse approaches for reducing catch limits to precautionarily buffer against scientific and management uncertainty, some of which may be more or less resilient to climate change. Here, we synthesize the control rules used to manage all 507 U.S. federally-managed fish stocks and stock complexes. We classified these rules into seven typologies: (1) catch-based; (2) constant catch; (3) constant escapement; (4) constant F; (5) stepped F; (6) ramped F, and (7) both stepped and ramped F. We also recorded whether the control rules included a biomass limit (“cutoff”) value or were environmentally-linked as well as the type and size of the buffers used to protect against scientific and/or management uncertainty. Finally, we review the advantages and disadvantages of each typology for managing fisheries under climate change and provide seven recommendations for updating harvest control rules to improve the resilience of U.S. federally-managed fisheries to climate change.
Bacteriological Assessment of Groundwater Quality Around a Major Municipal Dumpsite, Calabar, Se-Nigeria
Authors: OCHELEBE IBU , EKWERE AZUBUIKE , EDET ANIEKAN 
Published: August 2022

Abstract
Waterborne diseases are major public health concerns worldwide, not only for the morbidity and mortality they cause, but also for the huge cost of their prevention and treatment. These diseases are directly related to environmental deterioration and pollution, to which water is easily vulnerable. Assessment and water quality monitoring for microbial indicators in domestic water sources are key factors in preventing these health concerns. This study investigated the microbial indicators in groundwater around a major municipal waste dumpsite in Calabar metropolis in southeastern Nigeria. Twenty-nine (29) groundwater and seven (7) surface water samples were collected around the dumpsite across wet and dry seasons. The samples were analyzed for some indicator bacteria. The results show that the average counts for total bacteria were 5155 Cfu/100ml, total coliform bacteria 76 Cfu/100ml, total salmonella/shigella 56 Cfu/100ml, and total feacal coliform 53 Cfu/100ml in the dry season. In the wet season, the average count was: total bacteria 10,356 CFU/100ml, total coliform bacteria 468 Cfu/100ml, total salmonella/shigella 1081 CFU/100ml, total feacal coliform 28 Cfu/100ml, total vibrio cholera 433 CFU/100ml, and Escherichia coli 33 Cfu/100ml. Contamination levels are beyond permissible limits for indicator organisms in groundwater across seasons, with a higher frequency in the wet season, which pose a major health risk for consumers.
Vegetative cell fusion and a new stage in the life cycle of the Aphelida (Opisthosporidia)
Authors: Tcvetkova Victoria S. , Pozdnyakov Igor R. , Seliuk Alexei O. , Zorina Natalia A. , Karpov Sergey A. 
Published: August 2022

Abstract
The aphelids, intracellular parasitoid of algae, play significant role in the ponds communities regulating the algae blooms. They represent a large cluster of OTUs sister to Fungi at the molecular phylogenetic tree sharing a common ancestor with fungi. Their vegetative life cycle being similar to that of chytridiomycetes includes opisthokont uniflagellar zoospores, cysts, phagotrophic intracellular stage, and multinuclear plasmodium producing zoospores. The sexual part of life cycle is not known, and, as we can see now, the asexual part is also understudied. We have found in the Aphelidium insulamus and other ahelid species a new stage – big multiflagellar, or amoeboid cells, which can be considered both, the result of zoospore fusion, or the remnant of plasmodium after zoospore formation. A study of this phenomenon lead us to the conclusion, that after multicellular infection the trophic stages of different parasitoids fuse in one plasmodium having different sorts of nuclei. Some of them become the nuclei of zoospores, while the others left in the remnant of plasmodium, which becomes a monster. The families of protein encoded genes involved in the vegetative cell fusion was also discussed.
Perspectives of older people and healthcare providers on implementing person-centred care for community-dwelling older people: a systematic review and qualitative meta-synthesis
Authors: Liao Lulu , Liu Yilan , Guan Chunyan , Feng Mingjiao , You Yanjie , Chen Yuqin 
Published: August 2022

Abstract
Background
Person-centred care is a critical approach to improve the quality of care targeted to community-dwelling older people. The old-age care services could be provided according to the choices, needs and preferences of the elderly. However, there have been few studies to synthesize qualitative studies and no consensus about influencing factors on providing person-centred care in community. The aim of this study was to synthesize research evidence on perceptions and experiences of older people and healthcare providers on person-centred care and determine the enablers and barriers to implementing person-centred care for community-dwelling older people.
Methods
Searches were performed in PubMed, Embase, Cochrane, PsycINFO and CINAHL in published articles and were reviewed from the earliest available date to April 2022. A quality appraisal was undertaken targeted to selected articles by the Qualitative Method Appraisal Tool. And the results from the included studies were interpreted and synthesized through a meta-synthesis.
Results
Nine included articles were analyzed to identify 89 findings that were organized into 11 categories and combined into three synthesized findings—stakeholder capability; opportunities in the implementation of person-centred care; motivation in the implementation of person-centred care. Summarizing these themes helps stakeholders to identify influencing factors that improve the implementation of person-centred care.
Conclusions
The findings of this study provide a reference for implementing successful person-centred care in the community. Improving person-centred care service should develop the capability and motivation of stakeholders, and increase opportunities to build a positive environment for meaningful interactions between caregivers and older people.
A randomized, double-blinded, superiority clinical trial to compare two dimensions of traditional medical precision medicine for allergic rhinitis: syndrome differentiation-based treatment or staging treatment?
Authors: Li Yixue , Fu Wenyang , Chen Qiugu , Tong Tong , Xu Wenyu , Chen Ling , Liu Hengrui 
Published: August 2022

Abstract
Background Traditional Chinese medicine (TCM) is widely used for allergic rhinitis (AR) treatment. The precision medicine of TCM treatment included 1) syndrome differentiation-based treatment (SDT) and 2) staging-based prescription modifying treatment (ST). This study conducted a randomized, controlled, double-blinded, superiority, non-inferiority, and equivalence clinical trial to compare their therapeutic effect on AR.Methods Participants were diagnosed with TCM syndrome differentiation. Participants in the SDT group received a 6-week regular SDT with different prescriptions. Participants in the ST group received a 6-week ST in which every two weeks were defined as a stage (early, mid, and late) with different prescriptions. The prescriptions were all recommended by the Chinese National Administration of TCM and their therapeutic effects were supported by both classics of TCM literature and modern reference. The symptoms and constitutions related to AR patients were assessed at week 0 and week 6 and followed up after 1 year. SDT and ST in different gender, ages, and syndrome differentiation groups were compared. All the prescriptions in this study were analyzed using TCM components and property analysis and bioinformatic analysis.Results 158 SDT and 159 ST patients were included in the analysis. Both SDT and ST significantly improved symptoms and constitution of AR. ST had a better therapeutic effect than SDT except for “Lung meridian heat” patients. Although gender might not affect the difference between SDT and ST, age might be an impact factor. The TCM components and property analysis and bioinformatic analysis provide information to better understand these classical prescriptions.Conclusion For most AR patients, the syndrome differentiation based on disease alteration course over the treatment time was more therapy-critical than the syndrome differentiation based on patients’ constitution (or disease subtypes) and syndrome differentiation type and age are affecting factors.
D.SAP, an apple-based formulation could treat SARS-CoV-2 infection, and reduce associated inflammatory responses in COVID-19 infected mice model
Authors: Dezhakam Hossein , Dezhakam Amin , Dezhakam Ani , Haghighatfard Arvin 
Published: August 2022

Abstract
Introduction:
The SARS-CoV-2 virus is a virus that may cause severe respiratory disorders with an unclear rate of death. Natural vinegar may have an immune-boosting quality that effectively fights influenza-like respiratory infections. We aimed to evaluate the role of a specially formulated Iranian apple vinegar called Dezhakam sap (D.SAP) in the mice model of covid-19 in the treatment of infection and reduction of covid19-associated lung inflammation.
Methods:
We designed a covid-19-positive model of male inbred BALB/c mice by viral exposure. Then all mice were examined for active SARS-CoV-2 infection. Mice with positive covid-19 infection were separated into different groups to study the effect of treatment by different concentrations of D.SAP smoke for seven days. Expression assessments for viral RNA on covid-19 affected mice models for three SARS-CoV-2 genes (RDRP, N, and E) and three inflammation markers (IL6, IL1b, and TNF) in lung tissue of all mice were assessed using Real-time PCR.
Results:
Results showed a significant decrease in viral load in the RDRP gene, N gene, and E gene in all infected rats treated with D.SAP compared with off-treatment mice. In addition, all three inflammation factor genes in infected mice that were treated with D.SAP were significantly reduced compared to the off-treatment infected mice.
Conclusion:
Findings showed the effectiveness of specially formulated apple vinegar D.SAP to attack new coronavirus. In addition, results may suggest the effectiveness of D.SAP to reduce the lethal inflammation in COVID-19 infection with low side effects. Anti-inflammatory agents block certain substances in the body that cause inflammation. D.SAP may consider a potential natural anti-inflammatory that may increase the survival rate of COVID-19 infection. It seems these effects could be related to the antimicrobial and antioxidant effects of D.SAP due to its polyphenolic compounds.
Discovery of STAT3 Inhibitors: Recent Advances and Future Perspectives
Authors: Song Jiatao , Wang Jiawei , Tian Sheng , Li Huanqiu 
Published: August 2022

Background:
STAT3 (signal transducer and activator of transcription 3) is a
member of the STAT family of proteins that function as signal transducers and transcription
factors. Previous research has demonstrated its importance in cell proliferation, differentiation,
apoptosis, and immunological and inflammatory responses. Targeting the
STAT3 protein has recently been hailed as a viable cancer therapeutic method. Even
though none of these inhibitors have yet been exploited in clinical cancer therapy, a
small number have made them into clinical trials, leading researchers to explore more
promising inhibitors.

Methods:
Based on the mechanism of STAT3 activation, several types of STAT3 inhibitors
were described and summarized according to their origins, structures, bioactivity and
mechanism of action. Direct inhibition of STAT3 mainly targeted one of the three distinct
structural regions of the protein, namely the SH2 domain, the DNA binding domain,
and the coiled-coil domain.

Results:
The progress in STAT3 inhibitor discovery from 2010 to 2021 is comprehensively
summarized in this review. STAT3 inhibitors are mainly classified into small
molecule inhibitors, natural product inhibitors, and peptides/peptidomimetics. Moreover,
it also covers relevant analogues, as well as their core framework.

Conclusion:
Small-molecule inhibitors of STAT3 like BP-1-102 and BTP analogues displayed
great potential against various cancers, while natural products, as well as peptide
and peptidomimetics, also showed promising application. Therefore, STAT3 has become
a promising target with great research value, and the development of STAT3 inhibitors
may provide more therapeutic strategies for STAT3-related diseases.
Immune Checkpoint Inhibitors: Recent Clinical Advances and Future
Prospects
Authors: Banday Abid H. , Abdalla Mohnad 
Published: August 2022

Abstract:
Immune checkpoints are vital molecules and pathways of the immune system
with defined roles of controlling immune responses from being destructive to the healthy
cells in the body. They include inhibitory receptors and ligands, which check the recognition
of most cancers by the immune system. This happens when proteins on the surface
of T cells called immune checkpoint proteins identify partner proteins on the cancer cells
and bind to them, sending brake signals to the T cells to evade immune attack. However,
drugs called immune checkpoint inhibitors block checkpoint proteins from binding to
their partner proteins, thereby inhibiting the brake signals from being sent to T cells. This
eventually allows the T cells to destroy cancer cells and arbitrate robust tumor regression.
Many such inhibitors have already been approved and are in various developmental
stages. The well-illustrated inhibitory checkpoints include the cytotoxic T lymphocyte-associated
molecule-4 (CTLA-4), programmed cell death receptor-1 (PD-1), and programmed
cell death ligand-1 (PD-L1). Though many molecules blocking these checkpoints
have shown promise in treating many malignancies, such treatment options have
limited success in terms of the immune response in most patients. Against this backdrop,
exploring new pathways and next-generation inhibitors becomes imperative for developing
more responsive and effective immune checkpoint therapy. Owing to the complex biology
and unexplored ambiguities in the mechanistic aspects of immune checkpoint pathways,
analysis of the activity profile of new drugs is the subject of strenuous investigation.
We herein report the recent progress in developing new inhibitory pathways and potential
therapeutics and delineate the developments based on their merit. Further, the ensuing
challenges towards developing efficacious checkpoint therapies and the impending
opportunities are also discussed.
Centrosome Clustering & Chemotherapy
Authors: Saleem Rahman Shah Zaib , Firdous Farhat , Raza Hadeeqa Gull , Chotana Ghayoor Abbas , Choudhary M. Iqbal , Faisal Amir 
Published: August 2022

Abstract:
Centrosome abnormalities are the hallmark of cancer. How it affects tumorigenesis is
still a mystery. However, the presence of more than two centrosomes at the onset of mitosis often
leads to chromosomal instability and subsequent tumorigenesis. Unlike normal cells that undergo
repair or apoptosis in response to this instability, cancer cells learn to cope with supernumerary
centrosomes through various mechanisms and survive. Centrosome clustering is the most prevalent
mechanism, allowing the cancer cells to form two daughter cells through a pseudo-bipolar
spindle. Since healthy cells are devoid of the mechanisms involved in clustering, the de-clustering
of centrosomes can be considered a promising approach to selectively eliminate cells with extra
centrosomes. Several proteins such as PARP, KIFC1, Hsp70, Cortical actin, APC/C-CDH1 complex
and Eg5 have been discussed in this review which participate in centrosome clustering, and
the inhibition of these proteins can facilitate in impeding tumor growth specifically by declustering
centrosomes. In this review, we also present the role of the centrosome in the cell cycle, centrosome
amplification, clustering mechanism and reported centrosome de-clustering agents to present
the current state of work in the field.
The Effect of Bacterial Composition Shifts in the Oral Microbiota
on Alzheimer's Disease
Authors: Moghadam Majid Taati , Mojtahedi Ali , Bakhshayesh Babak , Babakhani Sajad , Ajorloo Parisa , Shariati Aref , Mirzaei Mehrnaz , Heidarzadeh Siamak , Jazi Faramarz Masjedian 
Published: August 2022

Abstract:
Alzheimer's disease (AD), a neurological disorder, despite significant
advances in medical science, has not yet been definitively cured, and the exact causes
of the disease remain unclear. Due to the importance of AD in the clinic, large expenses
are spent annually to deal with this neurological disorder, and neurologists warn of an
increase in this disease in elderly in the near future. It has been believed that microbiota
dysbiosis leads to Alzheimer’s as a multi-step disease. In this regard, the presence of
footprints of perturbations in the oral microbiome and the predominance of pathogenic
bacteria and their effect on the nervous system, especially AD, is a very interesting topic
that has been considered by researchers in the last decade. Some studies have looked
at the mechanisms by which oral microbiota cause AD. However, many aspects of this
interaction are still unclear as to how oral microbiota composition can contribute to this
disease. Understanding this interaction requires extensive collaboration by
interdisciplinary researchers to explore all aspects of the issue. In order to reveal the
link between the composition of the oral microbiota and this disease, researchers from
various domains have sought to explain the mechanisms of shift in oral microbiota in AD
in this review.