bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomal MiR-199a-5p Inhibits Tumorigenesis and Angiogenesis by Targeting VEGFA in Osteosarcoma
Authors: Zhang Lu , Cao Hongxin , Gu Guanghui , Hou Dehui , You Yunhao , Li Xiang , Chen Yunzhen , Jiao Guangjun 
Published: May 2022

BackgroundOsteosarcoma (OS) is the most common primary bone malignancy in children and adolescents. microRNAs have been found to play a vital role in tumor angiogenesis. Here, we investigated the effects of miR-199a-5p on tumor growth and angiogenesis in osteosarcoma. Furthermore, the underlying molecular mechanisms and signaling pathways were explored.MethodsThe datasets were extracted from the Gene Expression Omnibus and the differentially expressed miRNAs (DEmiRNAs) were screened out by the GEO2R online platform. The potential target genes were predicted using the miRTarBase database. The predicted target genes were further analyzed by Gene Ontology and pathway enrichment analysis and a regulatory network of DEmiRNAs and their target genes was constructed. In addition, the effects of osteosarcoma cell derived exosomal miR-199a-5p on the proliferation, migration and neovascularization of HUVECs were evaluated by conducting EdU assays, Transwell experiments and tube formation assays. A dual-luciferase reporter assay was performed to detect whether VEGFA was the direct target of miR-199a-5p. Furthermore, in vivo xenograft models were established to further investigate the intrinsic role of miR-199a-5p in osteosarcoma tumorigenesis and angiogenesis.ResultsA total of 149 DE-miRNAs were screened out, including 136 upregulated miRNAs and 13 downregulated miRNAs in human osteosarcoma plasma samples compared with normal plasma samples. A total of 1313 target genes of the top three upregulated and downregulated miRNAs were predicted. In the PPI network, the top 10 hub nodes with higher degrees were identified as hub genes, such as TP53 and VEGFA. By constructing the miRNA-hub gene network, we found that most of hub genes could be potentially modulated by miR-663a, miR-199a-5p and miR-223-3p. In addition, we found that the expression level of miR-199a-5p in exosomes derived from osteosarcoma cells was remarkably higher than the osteosarcoma cells, and the exosomes derived from osteosarcoma cells were transported to HUVECs. Overexpression of miR-199a-5p could significantly inhibited HUVEC proliferation, migration and neovascularization, whereas downregulation of miR-199a-5p expression exerted the opposite effect. Moreover, the in vivo results verified that overexpression of miR-199a-5p in osteosarcoma cells could suppress the growth and angiogenesis of tumors.ConclusionOur results demonstrated that miR-199a-5p could be transported from osteosarcoma cells to HUVECs through exosomes, subsequently targeting VEGFA and inhibiting the growth and angiogenesis of osteosarcoma. Therefore, miR-199a-5p may act as a biomarker in the diagnosis and treatment of osteosarcoma.
Complete Chloroplast Genome of the Inverted Repeat-Lacking Species Vicia bungei and Development of Polymorphic Simple Sequence Repeat Markers
Authors: Jo Ick-Hyun , Han Seahee , Shim Donghwan , Ryu Hojin , Hyun Tae Kyung , Lee Yi , Kim Daeil , So Yoon-Sup , Chung Jong-Wook 
Published: May 2022

BackgroundVicia bungei is an economically important forage crop in South Korea and China. Although detailed genetic and genomic data can improve population genetic studies, conservation efforts, and improved breeding of crops, few such data are available for Vicia species in general and none at all for V. bungei. Therefore, the main objectives of this study were to sequence, assemble, and annotate V. bungei chloroplast genome and to identify simple sequence repeats (SSRs) as polymorphic genetic markers.ResultsThe whole-genome sequence of V. bungei was generated using an Illumina MiSeq platform. De novo assembly of complete chloroplast genome sequences was performed for the low-coverage sequence using CLC Genome Assembler with a 200–600-bp overlap size. Vicia bungei chloroplast genome was 130,796-bp long. The genome lacked an inverted repeat unit and thus resembled those of species in the inverted repeat-lacking clade within Fabaceae. Genome annotation using Dual OrganellarGenoMe Annotator (DOGMA) identified 107 genes, comprising 75 protein-coding, 28 transfer RNA, and 4 ribosomal RNA genes. In total, 432 SSRs were detected in V. bungei chloroplast genome, including 64 mononucleotides, 14 dinucleotides, 5 trinucleotides, 4 tetranucleotides, 233 pentanucleotides, 90 hexanucleotides, and 14 complex repeated motifs. These were used to develop 232 novel chloroplast SSR markers, 39 of which were chosen at random to test amplification and genetic diversity in Vicia species (20 accessions from seven species). The unweighted pair group method with arithmetic mean cluster analysis identified seven clusters at the interspecies level and intraspecific differences within clusters.ConclusionThe complete chloroplast genome sequence of V. bungei was determined. This reference genome should facilitate chloroplast resequencing and future searches for additional genetic markers using population samples. The novel chloroplast genome resources and SSR markers will greatly contribute to the conservation of the genus Vicia and facilitate genetic and evolutionary studies of this genus and of other higher plants.
Circadian Effects on Attention and Working Memory in College Students With Attention Deficit and Hyperactivity Symptoms
Authors: Gabay Lily , Miller Pazia , Alia-Klein Nelly , Lewin Monica P. 
Published: May 2022

ObjectiveIndividuals with an evening chronotype prefer to sleep later at night, wake up later in the day and perform best later in the day as compared to individuals with morning chronotype. Thus, college students without ADHD symptoms with evening chronotypes show reduced cognitive performance in the morning relative to nighttime (i.e., desynchrony effect). In combination with symptoms presented in attention deficit hyperactivity disorder (ADHD), we predicted that having evening chronotype renders impairment in attention during the morning, when students require optimal performance, amplifying desynchrony.MethodFour hundred college students were surveyed for evening chronotype and symptoms of ADHD. Of those surveyed, 43 students with evening chronotype (19 with ADHD symptoms) performed laboratory attention tasks and were queried about fatigue during morning and evening sessions.ResultsStudents with ADHD symptoms demonstrated a greater decrement in sustained attentional vigilance when abstaining from stimulants and asked to perform cognitive tests at times misaligned with natural circadian rhythms in arousal compared to their non-ADHD counterparts with the same chronotype. While individuals with ADHD symptoms had slower reaction-times during sustained attention tasks in the morning session compared to those without symptoms, there was no significant group difference in working memory performance, even though both groups made more errors in the morning session compared to the evening session.ConclusionThese findings suggest that evening chronotype students with ADHD symptoms are at a greater disadvantage when having to perform sustained attention tasks at times that are not aligned to their circadian rhythm compared to their neuro-typical peers. The implications of this finding may be useful for the provision of disability accommodations to college age students with ADHD when they are expected to perform tasks requiring sustained attention at times misaligned with their circadian rhythms.
Inflammatory Cytokines, but Not Dietary Patterns, Are Related to Somatic Symptoms of Depression in a Sample of Women
Authors: Hazeltine Danielle Belden , Polokowski Ashley Rose , Reigada Laura Christine 
Published: May 2022

BackgroundDepression is a heterogenous disorder with both cognitive and somatic symptom dimensions that may differentially relate to systemic inflammation. Diet, which has the potential to modulate both inflammation levels and mood, is yet to be studied within the context of individual depression dimensions. This study examined the associations between inflammatory cytokines and dietary patterns with depressive symptom dimension profiles among a sample of women recruited in a non-clinical setting.MethodsInflammatory cytokines (IL-6 and TNF-α), inflammatory diet (Diet Inflammatory Index; DII), and depressive symptoms (Beck Depression Inventory-II; BDI-II) were measured in 136 females (Mage = 22.01 ± 4.02, range 18–59 years). Multiple linear regressions were used to investigate the relationships between inflammatory cytokines and diet with self-reported cognitive, somatic, and total depressive symptoms, adjusting for demographic factors.ResultsFindings showed that increased somatic dimension scores were positively associated with IL-6 (ß = 0.273, p = 0.002) and TNF-α (ß = 0.215, p = 0.017), but not inflammatory diet (p = 0.300). Total BDI-II scores were only positively associated with IL-6 (ß = 0.221, p = 0.012), and cognitive dimension scores were not associated with any inflammation measures.ConclusionsThese findings contribute to existing evidence that inflammatory cytokines are associated with the somatic symptoms of depression. Inflammatory diet index was not associated with depression measures.
Protocol for a Pragmatic Trial of Pharmacotherapy Options Following Unsatisfactory Initial Treatment in OCD (PROCEED)
Authors: Wang Pei , Gu Wenjie , Gao Jian , Wang Changhong , Fang Jianqun , Hu Maorong , Xiang Hui , Li Bin , Liu Na , Tang Wenxin , Wang Xijin , Jia Yanbin , Li Yi , Cheng Yuqi , Tang Zhen , Simpson Helen Blair , Stein Dan J. , Wang Zhen 
Published: May 2022

BackgroundSelective serotonin reuptake inhibitors (SSRIs) are the first-line pharmacotherapy for obsessive-compulsive disorder (OCD), but a large proportion of patients do not achieve remission after an adequate SSRI trial. To the best of our knowledge, there have been no well-powered randomized controlled trials (RCTs) of sequenced pharmacotherapy using pragmatic research designs. China provides a unique context for undertaking such a trial that will recruit the largest treatment-naïve participants and systematically compare the efficacy of different sequenced pharmacotherapy.MethodsA pragmatic research design will be adopted, with n = 1,600 treatment-naïve OCD patients initially treated for sertraline for 12 weeks, and with non-remitters then randomized to 5 different augmentation or switching pharmacotherapy options for another 12 weeks. The 5 arms will include: (1) treatment with higher than usual doses of sertraline, (2) switch to fluvoxamine, (3) switch to venlafaxine, (4) augmentation with memantine, and (5) augmentation with aripiprazole.DiscussionChina is uniquely positioned to recruit sufficiently large sample sizes of treatment-naïve OCD patients to compare different pharmacotherapy options; data from the proposed trial promises to help inform current clinical practice guidelines by providing important information about optimal pharmacotherapy choice for those who demonstrate no response or response but no remission to first line pharmacotherapy.Trial RegistrationThe trail was registered on 27 August 2020 in ClinicalTrials.gov (https://register.clinicaltrials.gov/) (NCT04539951).
Effect of Repeated Home Quarantine on Anxiety, Depression, and PTSD Symptoms in a Chinese Population During the COVID-19 Pandemic: A Cross-sectional Study
Authors: Tang Qiao , Wang Ya , Li Jing , Luo Dan , Hao Xiaoting , Xu Jiajun 
Published: May 2022

BackgroundStrict quarantines can prevent the spread of the COVID-19 pandemic, but also increase the risk of mental illness. This study examined whether the people who have experienced repeated home quarantine performance more negative emotions such as anxiety, depression, and post-traumatic stress disorder (PTSD) in a Chinese population.MethodsWe collected data from 2,514 participants in Pi County, Chengdu City, and stratified them into two groups. Group 1 comprised 1,214 individuals who were quarantined only once in early 2020, while Group 2 comprised 1,300 individuals who were quarantined in early 2020 and again in late 2020. Both groups were from the same community. The GAD-7, PHQ-9, and PCL-C scales were used to assess symptoms of anxiety, depression, and PTSD between the two groups.ResultsAnalyses showed that total PHQ-9 scores were significantly higher in Group 2 than in Group 1 (p &lt; 0.001) and the quarantine times and age are independent predictors of symptoms of depression (p &lt; 0.001). The two groups did not differ significantly in total GAD-7 or PCL-C scores.ConclusionIncreasing quarantine times was associated with moderate to severe depression symptoms, but not with an increase in symptoms of anxiety or PTSD.
Undertaking Rehabilitation Research During the COVID-19 Pandemic: Emergent Strategies From a Trainee-Faculty Workshop
Authors: Noguchi Kenneth S. , Nguyen Linda , Mehdipour Ava , Wiley Elise , Saunders Stephanie , Moncion Kevin , Reid Julie C. , Bakaa Nora , Garcia Diaz Laura , Van Damme Jill , D'Amore Cassandra , Kumurenzi Anne , Lu Ze , Knobl Erin , Beauchamp Marla K. , Macedo Luciana G. , Vrkljan Brenda , Moll Sandra E. , Carlesso Lisa C. , Letts Lori J. , Kho Michelle E. , Richardson Julie 
Published: May 2022

BackgroundThe COVID-19 pandemic has disrupted everyday rehabilitation research. Many academic institutions have halted in-person human research including rehabilitation sciences. Researchers are faced with several barriers to continuing their research programs. The purpose of this perspective article is to report the results of an interdisciplinary workshop aimed at understanding the challenges and corresponding strategies for conducting rehabilitation research during the COVID-19 pandemic.MethodsTwenty-five rehabilitation researchers (17 trainees and eight faculty) attended a 2-h facilitated online workshop in to discuss challenges and strategies they had experienced and employed to conduct rehabilitation research during the COVID-19 pandemic.ResultsRehabilitation researchers reported challenges with (1) pandemic protocol adjustments, (2) participant accessibility, and (3) knowledge dissemination, along with corresponding strategies to these challenges. Researchers experienced disruptions in study outcomes and intervention protocols to adhere to public health guidelines and have suggested implementing novel virtual approaches and study toolkits to facilitate offsite assessment. Participant accessibility could be improved by engaging community stakeholders in protocol revisions to ensure equity, safety, and feasibility. Researchers also experienced barriers to virtual conferences and publication, suggested opportunities for smaller networking events, and revisiting timeframes for knowledge dissemination.ConclusionThis perspective article served as a catalyst for discussion among rehabilitation researchers to identify novel and creative approaches that address the complexities of conducting rehabilitation research during the COVID-19 pandemic and beyond.
Circumferential Fusion Employing Transforaminal vs. Direct Lateral Lumbar Interbody Fusion—A Potential Impact on Implants Stability
Authors: Bokov Andrey , Kalinina Svetlana , Leontev Andrei , Mlyavykh Sergey 
Published: May 2022

BackgroundDifferent fusion techniques were introduced in clinical practice in patients with lumbar degenerative disc disease, however, no evidence has been provided on the advantages of one technique over another.The Objective of This StudyIs to assess the potential impact of circumferential fusion employing transforaminal lumbar interbody fusion (TLIF) vs. direct lateral interbody fusion (DLIF) on pedicle screw stability.Materials and MethodsThis is a single-center prospective evaluation of consecutive 138 patients with degenerative instability of lumbar spinal segments. Either conventional transforaminal lumbar interbody fusion (TLIF) with posterior fusion or direct lateral interbody fusion (DLIF) using cages of standard dimensions, were applied. The conventional open technique was used to supplement TLIF with pedicle screws while percutaneous screw placement was used in patients treated with DLIF. The duration of the follow-up accounted for 24 months. Signs of pedicle screws loosening (PSL) and bone union after fusion were assessed by the results of CT imaging. Fisher‘s exact test was used to assess the differences in the rate of CT loosening and revision surgery because of implant instability. Logistic regression was used to assess the association between potential factors and complication rate.ResultsThe rate of PSL detected by CT and relevant revision surgery in groups treated with TLIF and DLIF accounted for 25 (32.9%) vs. 2 (3.2%), respectively, for the former and 9 (12.0%) vs. 0 (0%) for the latter (p &lt; 0.0001 and p = 0.0043) respectively. According to the results of logistic regression, a decrease in radiodensity values and a greater number of levels fused were associated with a rise in PSL rate. DLIF application in patients with radiodensity below 140 HU was associated with a considerable decrease in complication rate. Unipolar or bipolar pseudoarthrosis in patients operated on with TLIF was associated with a rise in PSL rate while patients treated with DLIF tolerate delayed interbody fusion formation. In patients treated with TLIF supplementary total or partial posterior fusion resulted in a decline in PSL rate.ConclusionEven though the supplementary posterior fusion may considerably reduce the rate of PSL in patients treated with TLIF, the application of DLIF provide greater stability resulting in a substantial decline in PSL rate and relevant revision surgery.
Antibiotic Bowel Decontamination in Gastrointestinal Surgery—A Single-Center 20 Years’ Experience
Authors: Schardey Josefine , von Ahnen Thomas , Schardey Emily , Kappenberger Alina , Zimmermann Petra , Kühn Florian , Andrassy Joachim , Werner Jens , Arbogast Helmut , Wirth Ulrich 
Published: May 2022

ObjectiveAnastomotic leakage, surgical site infections, and other infectious complications are still common complications in gastrointestinal surgery. The concept of perioperative antibiotic bowel decontamination demonstrates beneficial effects in single randomized controlled trials (RCTs), but data from routine clinical use are still sparse. Our aim was to analyze the data from the routine clinical use of perioperative antibiotic bowel decontamination in gastrointestinal surgery.MethodsBased on 20 years’ experience, we performed a retrospective analysis of all cases in oncologic gastrointestinal surgery with the use of antibiotic bowel decontamination in gastric, sigmoid, and rectal cancer. Clinical data and perioperative outcomes were analyzed, especially regarding anastomotic leakage, surgical site infections, and other infectious complications.ResultsA total of n = 477 cases of gastrointestinal surgery in gastric cancer (n = 80), sigmoid cancer (n = 168), and rectal cancer (n = 229) using a perioperative regimen of antibiotic bowel decontamination could be included in this analysis. Overall, anastomotic leakage occurred in 4.4% (2.5% gastric cancer, 3.0% sigmoid cancer, 6.1% rectal cancer) and surgical site infections in 9.6% (6.3% gastric cancer, 9.5% sigmoid cancer, 10.9% rectal cancer). The incidence of all infectious complications was 13.6% (12.5% gastric cancer, 11.3% sigmoid cancer, 15.7% rectal cancer). Mortality was low, with an overall rate of 1.1% (1.3% gastric cancer, 1.8% sigmoid cancer, 0.4% rectal cancer). Antibiotic decontamination was completed in 98.5%. No adverse effects of antibiotic bowel decontamination could be observed.ConclusionOverall, in this large cohort, we can report low rates of surgery-related serious morbidity and mortality when perioperative antibiotic bowel decontamination is performed. The rates are lower than other clinical reports. In our clinical experience, the use of perioperative antibiotic bowel decontamination appears to improve patient safety and surgical outcomes during gastrointestinal oncologic procedures in a routine clinical setting.
Inerter Location-Based Vibration Suppression Study of a Transmission Line Equipped with Tuned-Mass-Damper-Inerter (TMDI) under Harmonic Excitation
Authors: Liu Xinpeng , Yang Yingwen , Sun Yi , Zhong Yongli , Zhou Lei 
Published: May 2022

This paper proposes a novel ungrounded TMDI to improve the vibration suppression performance of the transmission line under harmonic excitation. This type of inerter-based damper may transform a translational motion into a rotational motion, greatly increasing the efficiency of vibration suppression. In the present study, the differential equations of motion are first derived based on the transmission line with an ungrounded TMDI structure. Then the closed-form solution of the displacement response spectrum considering the influence of the suspension location of the inerter is developed. The impact of the inerter location on vibration suppression performance is investigated in depth by defining the suspension location factor (υ) and tuning the damping ratio and frequency ratio. The results demonstrate that the suspension location of the inerter has a substantial impact on the damping ratio, frequency ratio, and vibration suppression performance. When the connection location of the inerter is near to the mass of the damper, it degrades the vibration suppression performance of the system. The failure phenomenon of the inerter occurs in the range of 0.2 < υ < 0.3, indicating that the presence of the inerter in this range does not enhance vibration suppression performance. The modal coordinate difference has a considerable impact on the vibration suppression efficacy of the TMDI. With increasing modal coordinate differences, the vibration suppression performance of the TMDI grows dramatically.
Pengaruh Kompetensi Pegawai Dan Fasilitas Kerja Terhadap Kualitas Pelayanan Publik Di Kantor Desa Purworejo
Authors: Inzaghi Adelia Ovi , Rosy Brillian 
Published: May 2022

<p><em>Reformasi birokrasi Indonesia yang ditetapkan pada tahun 2010, seiring berjalannya waktu terus mengalami evaluasi untuk mengatasi beberapa masalah, salah satunya dalam peningkatan kualitas pelayanan publik. Berdasarkan observasi yang dilakukan </em><em>di lapangan masih adanya masyarakat yang termarginalisasikan (pengabaian atas hak-hak yang seharusnya didapat oleh masyarakat) baik dari pelayanan administrasi, hingga pelayanan yang lainnya. Sehingga dalam penelitian ini bisa dilaksanakan dengan tujuan untuk mengetahui pengaruh variabel kompetensi pegawai dan fasilitas kerja secara simultan maupun parsial terhadap kualitas pelayanan publik . Metode penelitian yang digunakan adalah kuantitatif, hal ini ditunjukkan oleh penggunaan teknik pengambilan sampel purposive sampling pada jumlah sampel sebanyak 31 responden. Dimana teknik ini mengambil metode kuesioner dengan menggunakan jenis pertanyaan tertutup. Analisis yang dihasilkan dari data yang digunakan adalah regresi linier berganda. Dengan memiliki hasil pada uji hipotesis bahwa kompetensi pegawai pada kualitas layanan publik ditunjukkan dengan </em> <em> </em><em>lebih besar dari nilai </em> <em> (7.058 &gt; 2.048) , sedangkan variabel fasilitas kerja terhadap kualitas pelayanan publik menunjukkan hasil </em> <em> lebih kecil dari nilai </em> <em> (0,139 &lt; 2.048), serta uji F memperoleh hasil </em> <em> mendominasi nilai </em> <em> sebesar (43,922 &gt; 3,34). Dari hasil tersebut maka dapat ditarik kesimpulan pada kompetensi pegawai menunjukkan bahwa terdapat pengaruh secara parsial terhadap kualitas pelayanan publik dan fasilitas kerja secara parsial tidak berpengaruh terhadap kualitas pelayanan publik serta secara simultan pada kompetensi pegawai dan fasilitas kerja berpengaruh terhadap kualitas pelayanan publik. Berdasarkan kesimpulan hasil penelitian, disarankan kantor desa mengadakan peningkatan bimbingan teknis, pelatihan manajerial kepada pegawai desa serta meningkatkan fasilitas kerja yang memadai dengan pengadaan teknologi guna meningkatkan kualitas pelayanan publik kepada masyarakat.</em></p>