bluuebunny/crossref_metadata_2025_split huggingface dataset:
E2F1 induced miR-652-3p promotes malignancy and metastasis of cancer cells via inhibiting TNRC6A in hepatocellular carcinoma
Authors: Chen Wei , Ru Junnan , Wu Tong , Man Da , Wu Jingbang , Wu Lijuan , Sun Yujing , Yu Hanxi , Li Min , Zhang Gangwei , Zhu Xingxin , Tong Rongliang , Xiao Heng , Li Yanhua , Yang Beng 
Published: June 2022

Abstract
Background
Metastasis serves as the leading cause of treatment failure and cancer-related death in HCC. The role of metastasis-associated miRNAs in HCC was elusive.
Methods
Combined analysis of TCGA data and exosomal miRNA sequencing data was performed. Expression of miR-652-3p in HCC was assessed. Function experiments of miR-652-3p and TNRC6A were performed both in vitro and in vivo. MRNA sequencing, PCR and western blot were performed to verify target genes and pathway of miR-652-3p. The lung metastasis and xenograft cancer model in nude mice was established to investigate the effects of miR-652-3p/TRNC6A axis on tumor metastasis in vivo. The potential transcription regulator of miR-652-3p was verified. The relationship of the expression of miR-652-3p/TNRC6A axis and prognosis of HCC patients was analyzed.
Results
Upregulated miR-652-3p was found in the tumor tissues of HCC, especially in metastatic HCC patients. Overexpression of miR-652-3p promoted and knockdown of miR-652-3p suppressed HCC metastasis both in vitro and in vivo. What’s more, miR-652-3p promoted HCC metastasis via regulating EMT pathway. Trinucleotide repeat-containing gene 6A protein (TNRC6A) was identified as a direct target of miR-652-3p, whose expression is negatively related with AFP level in HCC, and knockdown of TNRC6A promoted HCC metastasis. And knockdown of TNRC6A restored repressed EMT and HCC metastasis caused by inhibition of miR-652-3p. E2F1 transcriptionally promoted the expression of miR-652-3p through binding in the specific site in its DNA sequence. Besides, clinical results revealed that high expression of miR-652-3p and low expression of TNRC6A was positively correlated to shortened overall survival and disease-free survival in HCC patients.
Conclusions
The present study showed that E2F1 increases the expression of miR-652-3p, and miR-652-3p promotes EMT and HCC metastasis by directly inhibiting the expression of TNRC6A in HCC.
Assessment of ecosystem health status using benthic diversity as tools in coastal sediment off the southwest coast, India
Authors: Arya DB , Godson PS , Selvaraj P , Krishnakumar S , Magesh NS , Vincent Salom Gnana Thanga 
Published: June 2022

Abstract
The diversity of macro and meiobenthos was documented in order to assess the ecosystem health of an anthropogenically impacted coastal ecosystem for the first time along the southwest coast of Tamil Nadu. Offshore sediment and water samples (n = 63) were collected from 14 sampling stations using the grid sampling method. Water and sediment samples were analysed for environmental variables such as pH, temperature, electrical conductivity (EC), total dissolved solids (TDS), salinity, sulphate, dissolved oxygen (DO), carbohydrate (CHO), protein, lipid, labile organic matter (LOM), total organic carbon (Corg), total organic matter (TOM) and sediment texture using standard methods. Benthic macro and meiobenthos were isolated and identified using standard identification keys. A total of 5 phyla of macrobenthos with 29 species and 5 phyla of benthos with 60 species were identified in the study area. Relative abundance analysis showed that the largest numbers of species belonged to Polychaeta for macrobenthos and foraminifera for meiobenthos. Redundancy analysis indicated that sediment carbohydrate content was the highest determining factor for explaining variation in the diversity of benthos. AMBI & M-AMBI results revealed that among the 14 stations, 2 were categorized as “poor” and 9 as “bad”. Multiple anthropogenic activities in the coastal environment leading to increased organic matter input have resulted in a decline in the ecosystem health of this region, which warrants site-specific management to control organic pollution.
Secure Channel for Financial Transactions in Cloud Environment Using Blockchain Technology
Authors: D Prabakaran , Ramachandran Shyamala 
Published: June 2022

Abstract
The progression of digital technology impacts the users to migrate from traditional financial transactions to the online transaction system for performing their financial transactions. The existence of cloud computing and its major benefit of access from anywhere promotes financial transactions. The cloud infrastructure supporting performing financial transactions deals with the huge volume of data and it is quintessential to sustain a high level of security to those sensitive credentials in the cloud storage. The conventional cryptographic algorithms hold good in providing a better level of security to the sensitive data in cloud computing but still fall back to advanced attacks against the cloud data storage. Furthermore, the employment of conventional encryption algorithms in securing the cloud data consumes huge execution time, as it has to perform the authentication check for all types of user access including legitimate and non-legitimate accesses. This research article proposes a novel methodology of incorporating blockchain technology to perform online financial transactions in cloud computing with a high level of security, availability, and reduced execution time. This research work employs Blockchain technology integrated with Elliptical Curve Cryptography (ECC) and Chaotic Map Confusion and Diffusion algorithm to perform secured transactions over a cloud network. The interpretation of the proposed method contributes to better performance in the aforementioned parameters than the existing methodologies. The proposed framework is compared with the conventional method of online transactions using Multi-Factor Authentication (MFA) encompassing the Elliptical Curve Cryptography (ECC). The performance analysis proves that the proposed framework exhibits a high level of security along with reduced computational time, encryption, and decryption cost. The proposed work is interpreted with sample dataset and the computational time is reduced.
Temporal trends in staging and grading affecting risk of prostate cancer death in men with favourable-risk prostate cancer
Authors: Thomsen Frederik B. , Garmo Hans , Egevad Lars , Stattin Pär , Brasso Klaus 
Published: June 2022

Abstract
The survival benefit radical prostatectomy compared to observation in men with localised prostate cancer has been shown in two randomised trials primarily including men diagnosed in the 1990s. In more recent years, changes in work-up and histopathological assessments of prostate biopsies have changed, which has led to stage and grade migration. The aim of this study was to assess temporal trends in survival for men diagnosed with favourable-risk prostate cancer managed with radical prostatectomy or observation. We included men aged 75 or younger with Charlson Comorbidity index 0-1 diagnosed with favourable-risk prostate cancer (T1-T2, PSA <20 ng/mL and Gleason score 6 or 7 [3+4]) in the period 2000-2016 who underwent radical prostatectomy (n = 18 765) or observation (n = 21 149). Following propensity score matching, 9 664 men were selected in each treatment strategy. In more recent calendar periods, the absolute risk of prostate cancer death declined for both treatments. The absolute survival benefit of immediate radical prostatectomy compared to initial observation decreased: in men with low-risk prostate cancer the absolute difference was 1% in 2000-2005, 0.2% in 2006-2010, and 0.4% in 2011-2016 and for men with intermediate-risk prostate cancer 1.1% in 2000-2005, 0.6% in 2005-2010, and 0.7% in 2011-2016.
Green synthesis of Ag2O nanoparticles using Punica granatum leaf extract for sulfamethoxazole antibiotic adsorption: characterization, experimental study, modeling, and DFT calculation
Authors: Messaoudi Noureddine El , Mouden Abdelaziz El , Fernine Yasmine , Khomri Mohammed El , Bouich Amal , Faska Nadia , Ciğeroğlu Zeynep , Américo-Pinheiro Juliana Heloisa Pinê , Jada Amane , Lacherai Abdellah 
Published: June 2022

Abstract
In this study, silver oxide nanoparticles (Ag2O NPs) were generated by synthesizing green leaf extract of Punica granatum and used as adsorbent and used as adsorbent to remove the antibiotic additive sulfamethoxazole (SMX) from an aqueous solution. The chemical composition, surface morphology, and textural properties of the Ag2O NPs were identified using XRD, FTIR, BET/BJH, SEM-EDX, and TEM analyses. For 100 mg L− 1 SMX antibiotic concentration, Ag2O NPs achieved almost complete removal of 98.93% within 90 min by using 0.8 g L− 1 of adsorbent dose and initial solution pH of 4 at a temperature of 308 K. Langmuir model efficiently elucidates the experimental data, which amounts to homogeneous nature of antibiotic adsorption onto the Ag2O NPs. The maximum uptake capacity was 277.85 mg g− 1. Kinetic studies promise a PSO model. The ΔGº and ΔHº values confirm the spontaneity and endothermicity of the adsorption process. The regeneration study shows that the Ag2O NPs can be efficiently reused for up to five cycles. The geometric structures have been optimized and quantum chemical parameters were calculated for the SMX unprotonated (SMX+/−) and protonated (SMX+) using density functional theory (DFT) calculation, indicating that the SMX+/− is reacting more favorably on the surface of Ag2O NPs compared to the SMX+. According to this study, the Ag2O NPs as an excellent potential the adsorbent will be able to have remarkable effects in the treatment of pharmaceutical wastewater.
The application of apoptosis-related LncRNA markers in survival prediction, immunity and gene mutation analysis of advanced ovarian cancer was analyzed based on bioinformatics
Authors: fan weisen , qi yinghua , yuan zheng , zhang yingjie 
Published: June 2022

Abstract
OBJECTIVE:To investigate the potential prognostic value of necroptosis-associated lncRNAs (NALncRNAs)in patients with ovarian cancer.METHODS: Gene expression data from ovarian cancer patients with clinical data were downloaded from TCGA database. This is the base data for screening NALncRNAs that represent different survival times.lncRNA risk models were constructed by Cox regression analysis and evaluated for their prognostic value. The link between lncRNA signatures and related pathways, immune cell activity, immunological checkpoints, m6A-related genes, and gene mutations was also investigated. RESULTS: In this study, eight NALncRNAs including USP30-AS1, MINCR, LINC01096, DNM3OS, LINC02574, DTNB-AS1, ACAP2-IT1, and ILVBL-AS1, all of which were well represented for the outcome of ovarian cancer patients. Noemogram after calibration curve validation has good clinical utility. Risk signatures were shown to be abundant in various pathways related to immunology and cell proliferation, according to gene set enrichment analysis. Patients' late survival outcome corresponds to differences in immune cells, immune checkpoints, immune function, etc.A better immune microenvironment would facilitate late survival, but inexplicably patients with long survival times have a higher immune escape. Patients with high mutations in genes appear to have better survival outcomes.CONCLUSION: This study identified eight NALncRNAs markers for the first time, providing a valuable basis for more accurate prediction of ovarian cancer prognosis.
Biochemical control of DNA condensation
Authors: Agarwal Siddharth , Osmanovic Dino , Klocke Melissa , Franco Elisa 
Published: June 2022

Abstract
Phase separation of molecular condensates is emerging as a key mechanism in biology and biomaterials science. A major advantage of condensates is their capacity to form and reconfigure dynamically, generating responsive compartments that organize molecular targets and reactions in both space and time, in the absence of membranes. While condensation is known to depend on environmental conditions such as temperature and ionic strength, biological condensates in nature are likely influenced by fluctuating biochemical signals with high specificity. Here we ask whether the behavior of artificial condensates can be controlled via chemical reactions by design. Through theory and experiments we examine a model problem in which a phase separating component participates in chemical reactions that activate and deactivate its ability to self-attract. Our theoretical model indicates that such reactions have effects comparable to temperature, and illustrates the dependence of condensate kinetics on reaction parameters. We experimentally realize our model problem through a platform that combines DNA nanostar motifs to generate condensate droplets, and strand displacement reactions to kinetically control the nanostar valency. Our results show that DNA condensate dissolution and growth can be controlled reversibly via toehold-mediated strand displacement, and we characterize the influence of toehold and invasion domains, nanostar size, and nanostar valency. In some cases, the reduction of nanostar valency through invasion stabilizes the droplet size. Our results provide foundational methods for the development of dynamic nucleic acid condensates with potential applications in biomaterials science, nanofabrication, and drug delivery.
Usefulness of critical flicker fusion frequency measurement and its laterality for evaluating compressive optic neuropathy due to pituitary neuroendocrine tumors
Authors: Taguchi Akira , Kinoshita Yasuyuki , Tokumo Kana , Tominaga Atsushi , Kiuchi Yoshiaki , Yamasaki Fumiyuki , Horie Nobutaka 
Published: June 2022

Abstract
Objective
Critical flicker fusion frequency (CFF) is a short but sensitive method for evaluating optic nerve function. We measured CFF in patients with pituitary neuroendocrine tumors (Pit-NETs) to assess its usefulness.
Methods
Data from 184 patients with nonfunctioning Pit-NETs, who had been treated with transsphenoidal surgery and had no medical history of eye diseases, was used in this retrospective study. Visual acuity decline (VAD) was defined as > 0.10 reduction in logMAR visual acuity and CFF decline (CFD) was defined as CFF value < 35 Hz. Visual field defect (VFD) was evaluated by automated perimetry on a Humphrey visual field analyzer. Potential association between abnormal test results and tumor height from the suprasellar were analyzed.
Results
Contact between the optic nerve or chiasma and the tumor was present and absent in 161 and 23 patients, respectively. In patients showing contact, the difference in CFF between the left and right eyes was larger (p = 0.0008), and the optimal cut-off value using the receiver operating characteristic curve was 3 Hz. Therefore, ≥ 3 Hz was considered positive for CFF laterality (CFL), the most prevalent condition. Tumor height was lower in patients with CFL positivity compared to those with VAD or VFD (p < 0.01). The prevalence of test abnormalities was the highest for small tumors compared to those of other tests.
Conclusion
Changes in CFL permit early detection of Pit-NETs. Our results indicate that CFF laterality can be seen in the early stages of compressive optic neuropathy due to Pit-NET.
Development of enhanced primer sets for detection of norovirus and hepatitis A in food samples from Guayaquil (Ecuador) by reverse transcriptase-heminested PCR
Authors: SALAZAR E. J. , MIRABA M. J. , VILLAQUIRAN J. A. , AGUIRRE B. A. , SUÁREZ K. S. , CEVALLOS J. M. 
Published: June 2022

Abstract
Norovirus (NV) is a major viral pathogen that causes nonbacterial acute gastroenteritis and outbreaks of food-borne diseases. The genotype of NV responsible for the most frequent NV disease outbreaks is GII, accounting for 60–80% of the cases. Moreover, original, and new NV variants are continuously emerging, concomitant with the recent global increase in NV infections. Hepatitis A virus (HAV) is another foodborne pathogen frequently implicated in acute gastroenteritis cases around the world. The virus is transmitted among humans via the fecal-oral route, and infection by HAV causes the most serious form of viral illness acquired from foods. In this study, we implemented primer sets for the detection of NV genotypes I and II. We also developed primer sets for the detection of HAV. The primers were used in a heminested reverse transcriptase PCR (hnRT-PCR) protocol that was rapid and sensitive for the detection of NVG1, NVGII and HAV virus in food. The hnRT-PCR was applied successfully to strawberry and spinach obtained from a local fresh-food market, where we could detect NVGI, NVGII and HAV.
Study on the airway microecology of pulmonary tuberculosis based on 16S rRNA sequencing
Authors: Cai Xingshan , Luo Yang , Zhang Yuanliang , Lin Yuan , Wu Bitong , Cao Zhizhong , Hu Zuqiong , Wu Xingyi , Tan Shouyong 
Published: June 2022

Abstract
Background: Pulmonary tuberculosis is a chronic infectious disease of the respiratory system. It is still one of the causes of death from a single infectious disease, but it has been stuck in the study of a single pathogen. Recent studies have shown that many diseases are related to the microbiota. We clarified the occurrence of tuberculosis and the correlation between drug resistance and respiratory flora. High-throughput 16S rRNA gene sequencing was used to characterize the respiratory microbiota composition of 30 tuberculosis (TB) patients and compared with 30 health (H) controls. According to their Gene Xpert results, 30 pulmonary tuberculosis patients were divided into 12 persons in the sensitive group (DS0) and 18 persons in the drug-resistant group (DR0). The flora of the two were compared with the H group. Results: The data generated by sequencing showed that Firmicutes, Proteus, Bacteroides, Actinomyces and Fusobacterium were the five main bacterial phyla detected, and they constituted more than 96% of the microbial community. The relative abundance of Fusobacterium, Haemophilus, Porphyromonas, Neisseria, TM7, Spirochetes, SR1, and tunica in the TB group was lower than that of the H group, and Granulicatella was higher than the H group. The PcoA diagrams of the two groups had obvious clustering differences. The Alpha diversity of the TB group is lower than that of the H group, and the Beta diversity is higher than that of the H group (P<0.05). The relative abundance of Streptococcus in the DS0 group was much higher than that in the DR0 group (P<0.005).Conclusion: Pulmonary tuberculosis can cause disorders of the respiratory tract flora, in which the relative abundance of Streptococcus was significantly different between rifampicin-sensitive and drug-resistant patients.
Mechanistic insight into the tautomerization of histidine initiated by water-catalyzed N‒H and C‒H cleavages
Authors: xueying zhu , zijiao Chen , hongqi Ai 
Published: June 2022

Abstract
The N‒H and C‒H activation is of great significance in organic chemistry and chemical industry fields, especially, in the utilization of petroleum raw materials. High NδH (tautomer of natural histidine) content would increase Alzheimer’s disease risk. To inhibit this and improve the activation of N‒H and C‒H bonds, isomerization mechanism from NδH to NεH of histidine-containing dipeptide catalyzed by water cluster was explored. The results discovered that water cluster assists this reaction by reducing the activation energies from 68.20 to 9.60 kcal mol-1, and its size not only affects the reaction rate but also determines reaction pathway in a degree. Moreover, water cluster, taken as potential green catalyst, is more effective on the reactions involving N‒H and C‒H bond cleavages than reported common toxic organometallic compounds and have different catalytic mechanisms. This work also provide some theoretical guidance for the modulation of Alzheimer’s disease induced by histidine isomerization.
The green energy calculation of thirty-six square meters vane in different shape
Authors: Huang Junqiang 
Published: June 2022

Abstract
We design a new tidal power station as Fig. 1, including one impeller with the vane length 6 meters and the vane width 6 meters,and the impeller shaft above the sea water surface 1 meter. When the sea water flow speed is 2(m/s),and the impeller speed is 1.3 (rpm),we can get the green energy of this power station 225.1(KW).When the sea water flow speed is 3(m/s),and the impeller speed is 2(rpm),we can get the green energy 766(KW).When the sea water flow speed is 4(m/s),and the impeller speed is 3 (rpm),we can get the green energy 1818(KW).We build a table as the following Table 1. We design a new tidal power station as Fig. 1, including one impeller with the vane length 12 meters and the vane width 3 meters,and the impeller shaft above the sea water surface 1 meter.When the sea water flow speed is 2(m/s),and the impeller speed is 1.3 (rpm),we can get the green energy 32.9(KW).When the sea water flow speed is 3(m/s),and the impeller speed is 2(rpm),we can get the green energy 339(KW).When the sea water flow speed is 4(m/s),and we presume the impeller speed is 3 (rpm),we can get the green energy 853(KW).We are happy to see the value is considerable,and we can build a table as the following Table 2.
Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis
Authors: Kim Ok-Hyeon , Choi Young Wook , Hong Soon Auck , Hong Mineui , Chang In Ho , Lee Hyun Jung 
Published: June 2022

Abstract
Background
Mechanical forces surrounding solid tumors are pervasive in the tumor microenvironment (TME) and abnormally altered as solid tumors progress. Although it has been reported that biomechanical forces, including wall shear stress (WSS), enhance the metastatic features of cancer cells, the mechanism by which cancer cells sense mechanical stress and propagate signals in the TME remains unknown.
Methods
Using a microfluidic device, interstitial fluid-mimicking flow (0.05 dyne cm− 2) was applied to the human prostate cancer cell line PC3. Piezo1 expression was detected using immunofluorescence and qRT-PCR. Piezo1 siRNA and shRNA lentivirus were applied to PC3 cells to ablate Piezo1 expression. PC3-Luc2 cells expressing control shRNA or shPiezo1 lentivirus were administered into the prostate of BALB/c mice for orthotopic injection, and metastatic prostate cancer cells were tracked by in vivo imaging for 6 weeks.
Results
Here, we show that Piezo1, a mechanosensitive ion channel, is activated by WSS in microfluidic channels. Moreover, Yoda1, a Piezo1 agonist, synergistically potentiates cancer cell motility via WSS. Yoda1 further increased WSS-mediated translocation of YAP/TAZ from the cytosol to the nucleus compared to WSS alone. Conversely, depleting Piezo1 with siRNA significantly reduced cell motility induced by WSS and inhibited nuclear retention of YAP/TAZ. Moreover, activation of Piezo1 increased phosphorylation of Src on Y416, which regulates phosphorylation of YAP on Y357, whereas ablation of Piezo1 with siRNA failed to induce Src phosphorylation and YAP activation. Furthermore, mice bearing control PC3-Luc2 showed rapid tumor growth and metastasis throughout the body, while this gradually decreased with the injection of shPiezo1 lentivirus into control PC3-Luc2-bearing BALB/c mice.
Conclusions
Taken together, these results demonstrate that Piezo1 allows cancer cells to sense mechanical stimuli by altering the microenvironment during tumor progression and is a critical player in modulating cancer metastasis through the Piezo1-Src-YAP axis.