bluuebunny/crossref_metadata_2025_split huggingface dataset:
The arginine methyltransferaseCarm1is necessary for heart development
Authors: Jamet Sophie , Ha Seungshin , Ho Tzu-Hua , Houghtaling Scott , Timms Andrew , Yu Kai , Paquette Alison , Maga Ali Murat , Greene Nicholas D E , Beier David R 
Published: June 2022

AbstractTo discover genes implicated in human congenital disorders, we performed ENU mutagenesis in the mouse and screened for mutations affecting embryonic development. In this work, we report defects of heart development in mice homozygous for a mutation of coactivator-associated arginine methyltransferase 1 (Carm1). While Carm1 has been extensively studied, it has never been previously associated with a role in heart development. Phenotype analysis combining histology and microcomputed tomography imaging shows a range of cardiac defects. Most notably, many affected midgestation embryos appear to have cardiac rupture and hemorrhaging in the thorax. Mice that survive to late gestation show a variety of cardiac defects, including ventricular septal defects, double outlet right ventricle, and persistent truncus arteriosus. Transcriptome analyses of the mutant embryos by mRNA-seq reveal the perturbation of several genes involved in cardiac morphogenesis and muscle development and function. In addition, we observe the mislocalization of cardiac neural crest cells at E12.5 in the outflow tract. The cardiac phenotype of Carm1 mutant embryos is similar to that of Pax3 null mutants, and PAX3 is a putative target of CARM1. However, our analysis does not support the hypothesis that developmental defects in Carm1 mutant embryos are primarily due to a functional defect of PAX3.
G1-Cyclin2 (Cln2) promotes chromosome hypercondensation in eco1/ctf7 rad61 null cells during hyperthermic stress in Saccharomyces cerevisiae
Authors: Buskirk Sean , Skibbens Robert V 
Published: June 2022

Abstract
Eco1/Ctf7 is a highly conserved acetyltransferase that activates cohesin complexes and is critical for sister chromatid cohesion, chromosome condensation, DNA damage repair, nucleolar integrity, and gene transcription. Mutations in the human homolog of ECO1 (ESCO2/EFO2), or in genes that encode cohesin subunits, result in severe developmental abnormalities and intellectual disabilities referred to as Roberts syndrome and Cornelia de Lange syndrome, respectively. In yeast, deletion of ECO1 results in cell inviability. Codeletion of RAD61 (WAPL in humans), however, produces viable yeast cells. These eco1 rad61 double mutants, however, exhibit a severe temperature-sensitive growth defect, suggesting that Eco1 or cohesins respond to hyperthermic stress through a mechanism that occurs independent of Rad61. Here, we report that deletion of the G1 cyclin CLN2 rescues the temperature-sensitive lethality otherwise exhibited by eco1 rad61 mutant cells, such that the triple mutant cells exhibit robust growth over a broad range of temperatures. While Cln1, Cln2, and Cln3 are functionally redundant G1 cyclins, neither CLN1 nor CLN3 deletions rescue the temperature-sensitive growth defects otherwise exhibited by eco1 rad61 double mutants. We further provide evidence that CLN2 deletion rescues hyperthermic growth defects independent of START and impacts the state of chromosome condensation. These findings reveal novel roles for Cln2 that are unique among the G1 cyclin family and appear critical for cohesin regulation during hyperthermic stress.
How Can We Resolve Lewontin’s Paradox?
Authors: Charlesworth Brian , Jensen Jeffrey D 
Published: June 2022

AbstractWe discuss the genetic, demographic, and selective forces that are likely to be at play in restricting observed levels of DNA sequence variation in natural populations to a much smaller range of values than would be expected from the distribution of census population sizes alone—Lewontin’s Paradox. While several processes that have previously been strongly emphasized must be involved, including the effects of direct selection and genetic hitchhiking, it seems unlikely that they are sufficient to explain this observation without contributions from other factors. We highlight a potentially important role for the less-appreciated contribution of population size change; specifically, the likelihood that many species and populations may be quite far from reaching the relatively high equilibrium diversity values that would be expected given their current census sizes.
Validation of the 3M™ Molecular Detection Assay 2—STEC Gene Screen (stx and eae) for Detection of Shiga Toxin Gene (stx1 and/or stx2) and Intimin Gene (eae) in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested MethodSM 071902
Authors: Rosauer Micki L , Lopez-Velasco Gabriela , Silbernagel Karen M , Horine Leo , Tudor Alexandra 
Published: June 2022

Abstract

Background
The 3M™ Molecular Detection Assay 2—STEC Gene Screen (stx and eae) method is based on gene amplification by the use of real time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Shiga toxin gene (stx1 and/or stx2) and intimin gene (eae) from Shiga toxin-producing Escherichia coli (STEC) in enriched products. The 3M Molecular Detection Assay 2—STEC Gene Screen (stx and eae) was approved as AOAC Performance Tested MethodSM Certificate No. 071902.

Objective
This matrix extension study evaluated the 3M Molecular Detection Assay 2—STEC Gene Screen (stx and eae) method for detection of STECs in dried cannabis flower [&gt;0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size.

Method
Testing followed procedures outlined in 3M Molecular Detection Assay 2—STEC Gene Screen (stx and eae) product instructions and Standard Method Performance Requirements (SMPR®) for Detection of Shiga Toxin-Producing Escherichia coli in Cannabis and Cannabis Products (AOAC SMPR 2020.012). The method was evaluated at low, high, and non-inoculated levels.

Results
Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2—STEC Gene Screen (stx and eae) results and the SMPR 2020.012 recommended cultural confirmations.

Conclusions
This study provides data that demonstrate the 3M Molecular Detection Assay 2—STEC Gene Screen (stx and eae) is a reliable method for the rapid and specific detection of STEC organisms in dried cannabis flower and dried hemp flower.

Highlights
The 3M Molecular Detection Assay 2—STEC Gene Screen (stx and eae) method is suitable for the rapid and specific detection of STEC organisms in dried cannabis flower and dried hemp flower.
Rare case of concurrent suprasternal and cardiac metastasis from small bowel neuroendocrine tumour
Authors: Nan Xinyi , Dharmawardhane Anoj 
Published: June 2022

Abstract
Neuroendocrine tumours (NETs) are rare tumours derived from the neuroendocrine cell system, arising across a wide range of organs, most commonly the gastrointestinal tract and bronchopulmonary symptoms. Although NETs can metastasis widely throughout the body, cardiac metastasis is rare with an incidence of 2–4% and usually presents in the presence of extensive metastasis elsewhere. Suprasternal metastasis to the neck is exceedingly rare with &lt;20 cases reported in the literature. We report the case of a 71-year-old female with concurrent cardiac and suprasternal metastasis at diagnosis of terminal ileal NET.
Getting and Feeling Safer, Part 1
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
This module focuses on recognizing and interrupting patterns that increase the risk of doing things that are risky, unhealthy, or unsafe. It also offers things you can do to help yourself get and feel safer. As part of this work, you will learn why getting your healthy needs met safely is so important for your healing, and we will help you recognize what your healthy needs are and how to work toward meeting them. You will also learn how to recognize and manage challenging situations and how to notice and respond to early warning signs in order to break the cycle of unhealthy behavior and reduce trauma-related reactions.
Sticking With the Process and Building on Progress
Authors: Schielke Hugo J. , Brand Bethany L. , Lanius Ruth A. 
Published: June 2022

Abstract
Over the course of this program, you’ve been learning how to help yourself heal from the effects of trauma, including learning healthy ways to manage trauma-related symptoms. You’ve also been learning how to have healthy relationships with your emotions and all of who you are—including increasing self-compassion, getting your healthy needs met safely, and giving yourself the care you need. This module will help you learn how to keep making progress toward feeling safer, including feeling safe while feeling your feelings, how to help yourself be OK with feeling good, and how to keep healing. It will also guide you in noticing and giving yourself credit for what you have been doing, identifying and addressing factors that may be keeping you from feeling safer and better, and recognizing the changes you’ve made. Finally, you can think about what you would like to revisit and practice more.
Purposes and Premises
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThis chapter establishes definitions. Cognition is an inclusive term, while consciousness, a moment-to-moment product of cognition, is narrower in definition. The three interrelated approaches of this book are introduced: (1) positive psychotherapy; (2) the “common factors” of therapeutic effectiveness; and (3) a Jaynesian therapeutic paradigm. Six aspects of a Jaynesian therapeutic paradigm are postulated: (1) nonconscious mentation; (2) the inherent self-healing potential of the features/functions of conscious interiority (FOCI); (3) mental imagery as an artifact of adaptive hallucinations; (4) lessons from altered cognition (hypnosis, i.e., the therapeutic benefits of turning consciousness off); (5) metaphors as an example of meta-framing; and (6) emotions as interiorized affects. Other themes of this book include: temporality and the adaptive psyche; historical changes in psychological processes; adaptive phasic structures (APSs); the self as systemized, serialized, and dramatized; and multiculturalism. This book’s purpose is not to offer new therapeutic techniques or recommend certain therapies over others.
A Brief History of the Interiorization of Psychotherapy
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThe nature of conscious interiority can be better appreciated if one traces how it configured the development of psychotherapy. In other words, this chapter historically contextualizes the relations between conscious interiorization and the emergence of treatments and interventions. It begins by examining the psychoanalytic discovery of the “psychological”; neo-psychoanalytic contributions; object relations theory; how behaviorism discarded introspection and conscious interiority; the legacy of behaviorism and neo-behaviorism; and the shift in treatment focus from objective behavior to subjective experience. This chapter also investigates the three waves of: (1) behavior therapy; (2) cognitive behavior and rational emotive behavior therapies; (3) and acceptance and commitment and dialectical behavior therapies. The interiorization of the humanistic tradition (client-centered therapy, existential therapy, and Gestalt therapy), as well as the downside of conscious interiorization, are also explored.
The Nature of Self
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThis chapter, to better appreciate the nature of selfhood, approaches it from several angles. First, it is viewed as part of a system (systemized, i.e., the networked psyche as informed by a systems theory approach). Second, the self is investigated as a sequential, serialized, and narratizable entity. Third, the “staginess” of the self is introduced; this dramatized angle sets the groundwork for the role-self perspective (RSP) or how one performs scripts that give life to oneself. As there is no such thing as a completely socially detached role, selves are inherently theatrical and “multi-roled” within the context of social relations. In this sense, selves interlink an individual to family, friends, and wider social networks. RSP is grounded in the therapeutic centrality of gaining self-objectivity through distancing one’s observing “I” and observed “me”; this is achieved through role-playing. This chapter demonstrates the linkages between RSP, psychodrama, and positive psychology.
Key Therapeutic Skills and Conscious Interiority
Authors: McVeigh Brian J. 
Published: June 2022

AbstractAfter introducing the significance of the therapeutic alliance and the healing power of socioaffiliative presence, this chapter examines the art of counseling from a dramaturgical perspective, listing the requisite basic skills and techniques. It explores how the therapeutic role is learned by the mental health care provider; the vital importance of active listening; paying close attention to the feelings of others; the self-presentation of the therapist; providing feedback; staying focused on the interaction between the therapist and the patient; observation skills and collecting information; the usefulness of silence; the advantages and benefits as well as the disadvantages and risks of self-revelation; third-person psychotherapy; and the dynamics of the therapist‒patient feedback loop.
Human Diversity, Social Adaptation, and Psychotherapy
Authors: McVeigh Brian J. 
Published: June 2022

AbstractThis chapter explores the intersection of multiculturalism, conscious interiority, and therapy. Its premise is that “every person is a culture,” i.e., all counseling is cross-cultural. Each person—being a dynamic, complex constellation of socioeconomic factors, ethnocultural influences, sexual and gendered identities, spiritual sentiments, personal predispositions, familial traditions—possesses his or her own culture. The nature of this “individualized culture” (self-individuation) must be appreciated when treating a patient within a clinical setting. This chapter’s core argument distinguishes between the uses and misuses of identity. This is achieved by breaking “identity” down into: identification and categorization by others; interiorizing identity and self-understanding; commonality and sameness; emotional connectedness; and groupness (belonging to a distinctive, bounded, solidary group). This chapter also explains individualizing the collectivity (carelessly applying monolithic characterizations that stereotype); collectivizing the individual (misuses and misapplications of group identity); biases and nonconscious cognition; the need to acknowledge macro-contextual forces; and what is required to be a multiculturally sensitive therapist.
Adolescent medicine subspecialty workforce: Insights from Canada
Authors: Roy-Lavallée Julien , Findlay Sheri M , Chen Allison , Katzman Debra K 
Published: June 2022

Abstract

Objectives
Adolescent Medicine (AM) in Canada has undergone significant growth since being accredited by the Royal College of Physicians and Surgeons of Canada (RCPSC) in May 2007. A deeper understanding of the workforce is needed in order to identify current gaps, to improve clinical care and scholarly endeavors, and to inform future developments.

Methods
This is the first AM workforce survey administered in Canada and included 39 multiple-choice and 3 open-ended questions. Descriptive statistics were calculated, and thematic analysis was used for open-ended questions.

Results
We identified 62 AM specialists from across Canada. The overall response was 97% (60/62). Most AM specialists were women (39/53, 74%), Caucasian (38/53, 72%), between 30 and 39 years old (22/53, 42%), and completed their subspecialty training in either Toronto (24/48, 50%) or Montreal (12/48, 25%). Nearly half of participants worked in either the Toronto, Ontario (13/49, 27%) or Montreal, Quebec (10/49, 20%). Nearly all participants (46/49, 94%) practiced in large urban population centres and were based in academic health science centres. The primary clinical areas of focus included eating disorders (25/51, 49%) and mental health (9/51, 18%). Almost all participants were satisfied with their career choice (41/50, 82%). Two-thirds of the participants (31/48, 65%) believed that there was an insufficient number of AM specialists in Canada.

Conclusions
Highlighting current characteristics of the AM subspecialty will help government and academic policymakers in understanding the workforce available to care for Canadian adolescents and the need to develop training programs and policies to address gaps and shortages.
Examining the lineage autonomous role of β3‐integrin in muscle regeneration
Authors: Gerassimov Nathalie , Crain Colt , Bilyeu Colin , Jacob Andrew , Fan Chen‐Ming 
Published: June 2022

AbstractSkeletal muscles can regenerate over the lifetime from resident muscle stem cells (MuSCs). Interactions between MuSCs and extracellular matrix (ECM) proteins are essential for muscle regeneration. The best‐known receptors for ECM proteins are integrins, a family composed of twenty‐some heterodimeric combinations of an α‐ and a β‐subunit. β1‐integrin (encoded by Itgb1) is required for quiescence, proliferation, migration, and fusion of Pax7+ MuSCs in the mouse model. β3‐integrin (encoded by Itgb3) has been reported to be critical for the myogenic differentiation of C2C12 myoblasts, and Itgb3 germline mutant mice were shown to regenerate few if any myofibers after injury. To investigate the autonomous role of Itgb3 in the myogenic lineage in vivo, we conditionally inactivated a floxed Itgb3 allele (Itgb3F) by constitutive Pax7‐Cre and tamoxifen‐inducible Pax7‐CreERT2 drivers. Unexpectedly, we found no defects in muscle regeneration in both conditional knockout models. In vitro studies using Itgb3 mutant myoblasts or RNAi knockdown of Itgb3 in myoblasts also did not reveal a role for myogenic differentiation. As β1‐ and β3‐integrins share ECM ligands and downstream signaling effectors, we further examined Itgb3's role in a Itgb1 haploid background. Still, we found no evidence for an autonomous role of Itgb3 in muscle regeneration in vivo. Thus, while Itgb3 is critical for the differentiation of C2C12 cells, the regenerative defects reported for the Itgb3 germline mutant are not due to its role in the MuSC. We conclude that if β3‐integrin does have a role in Pax7+ MuSCs, it is compensated by β1‐ and/or another β‐integrin(s).
Analysis of Scoliosis-related Medical Services in South Korea Between 2010 and 2018
Authors: Hong Doohyun , Lim Yu-Cheol , Lee Sook-Hyun , Sung Won-Suk , Lee Yoon Jae , Kim Eun-Jung , Ha In-Hyuk 
Published: June 2022

Study Design.
Retrospective, cross-sectional study.

Objectives.
The aim of this study was to analyze types and cost of care for scoliosis and present health care utilization by patients to provide data on the standard treatment and cost for scoliosis in South Korea.

Summary of Background Data.
Scoliosis is defined as an abnormally laterally curved spine with a Cobb angle of ≥10°.Data are lacking on which treatments are popular for general scoliosis and which service categories incur the most cost in South Korea.

Methods.
Data from the Health Insurance Review and Assessment Service-National Patient Sample were used. In total, 108,643 final cases and 32,362 patients in South Korea who received Western medicine care or Korean medicine care at least once with scoliosis (ICD-10 code M41) as the principal diagnosis during January 2010 to December 2018 comprised the study sample. We analyzed health care utilization according to certain factors (e.g., age, sex, payer type, type of visit, and medical institution), health care utilization by service category and service code, and medication prescriptions filled by private or hospital pharmacies.

Results.
Patients aged 24 years or younger most frequently utilized health care services, with more female (64.84%) than male patients (35.16%). Most patients received only Western medicine care (92.18%), whereas 6.56% only received Korean medicine care. Most cases (99.37%) corresponded to outpatient. Regarding service category, treatment and surgery, examination, special equipment and radiologic diagnosis accounted for a substantial percentage. Regarding service code, cost of examination, imaging, and physiotherapy comprised a high percentage of Western medicine, whereas cost of examination, acupuncture, and cupping therapy accounted for a high percentage of Korean medicine. The most commonly prescribed medications for scoliosis in pharmacies were anti-inflammatory analgesics (nonsteroidal anti-inflammatory drugs), antacids, and skeletal muscle relaxers.

Conclusion.
The findings can be used as foundational data for experts such as health care policymakers, clinicians, and researchers.

Level of Evidence: 4
Sutureless Lyophilized Amniotic Membrane Grafting for Corneal Epithelial Defects
Authors: Delbarre Maxime , Boucenna William , Froussart-Maille Françoise 
Published: June 2022

Objectives:
To report the tolerability, safety, and efficacy of the lyophilized amniotic membrane (AM) Visio-AMTRIX placed under a bandage contact lens (BCL) in treating persistent epithelial defects (PEDs).

Methods:
This retrospective study included consecutive patients with PEDs treated with a lyophilized AM placed under the BCL. Patients with PEDs who did not respond to medical treatment were included. Patients with a follow-up time less than 3 months were excluded.

Results:
Eleven eyes of 11 patients (mean age, 61.6±15.9 years) were included. Time from PED presentation to AM transplantation (AMT) was 27.7±4.9 days, with the mean PED area of 13.2±11.3 mm2. Complete resolution was achieved in 8 of 11 eyes after a single AM graft. The epithelial defect persisted after the first AMT in three eyes (27.3%), and a second graft was necessary to achieve complete healing. The corneal epithelial defect healed in an average of 11.0±4.4 days after grafting. After PED resolution, the best-corrected visual acuity significantly improved from 0.66±0.30 logMAR (20/91 Snellen) to 0.58±0.24 logMAR (20/77 Snellen) (P=0.036) compared with baseline. The AM resorbed within 2 weeks in all cases. No complication or recurrence was observed.

Conclusions:
A sutureless lyophilized AM under the BCL can resolve PEDs with a significant improvement in vision.
Impacts of the Statewide COVID-19 Lockdown Interventions on Excess Mortality, Unemployment, and Employment Growth
Authors: Pugh Trevor , Harris Jeffrey , Jarnagin Kyle , Thiese Matthew S. , Hegmann Kurt T. 
Published: June 2022

ObjectiveThe aim of the study is to determine relationships between lockdowns and excess mortality, unemployment, and employment growth.MethodsEach US states’ mortality data for 2020 were compared with the prior 3 years to determine excess mortality. Data were compared using measures of lockdowns, or state openness scores and adjusted for age, sex, race/ethnicity, and cardiovascular disease. Comparisons were made with unemployment rates and employment growth rates.ResultsThe 2020 excess mortality ranged from −9% to 46%. The average openness score was not significant (P= 0.20). However, openness was strongly associated with both unemployment (P= 0.01) and employment growth (P= 0.0008).ConclusionsThere was no statistical relationship between excess mortality and openness scores, while there were strong relationships with employment measures. These results suggest that lockdowns are not sufficiently beneficial for future use in this pandemic and raise concerns for use in future pandemics.