bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of miR-4510 as a metastasis suppressor of gastric cancer through regulation of tumor microenvironment via targeting GPC3
Authors: Ma Hai-fen , Shu Peng , Shi Xiao-hai , Wang Min , Jiang Mao-fen 
Published: January 2022

AbstractThe genes miR-4510 and glypican-3 (GPC3) have reported to be closely associated with tumors, with miR-4510 inversely correlated with GPC3 mRNA and protein in hepatocellular carcinoma samples. Glypican-3-expressing gastric cancer (GPC3-GC), characterized as gastric cancer (GC) expressing GPC3, accounts for 11% of the GC cases. However, the expression and mechanism of action of miR-4510 in GPC3-GC have not been clearly defined. We found that miR-4510 expression in GC tissues was significantly lower than that in the adjacent tissues (p < 0.001). miRNA-4510 expression in GPC3-GC was significantly lower than that in GPC3‐negative GC tissue (p < 0.001). Our study confirmed that miR-4510 is inversely correlated with GPC3 in gastric cancer samples and that GPC3 is a direct target gene of miR-4510. The proportion of M2 macrophages in GC with low expression of miR-4510 was significantly increased, while the proliferation of CD8+ T cells was limited. miR-4510 may change the immunosuppressive signals in the tumor microenvironment by downregulating GPC3 and inhibiting gastric cancer cell metastasis. Oxaliplatin treatment may become a specific therapeutic drug for patients with miR-4510 inhibition and GPC3-GC.
A New American University Model for Training the Future MCH Workforce Through a Translational Research Team
Authors: Whisner Corrie M. , Brown Jean C. , Larson David M. , Rodriguez Lizeth Alonso , Peter Beate , Reifsnider Elizabeth , Bever Jennie , Liu Li , Raczynski Erin , Chavez Jose-Benito Rosales , Ojinnaka Chinedum , Berkel Cady , Bruening Meg 
Published: January 2022

Abstract
Objectives
To describe the process of developing and implementing experiential learning through translational research teams that engage diverse undergraduate and graduate students.

Methods
After a college redesign, translational research teams were developed to foster multidisciplinary research and better integrate students with faculty research, community, and clinical activities. Three primary approaches were used to engage undergraduate and graduate students in the maternal and child health translational research team (MCH TrT). These included an undergraduate experiential learning course; participation in translational research team meetings and events; and mentorship activities including graduate student theses and supplementary projects.

Results
Since 2019, a total of 56 students have engaged with the MCH translational research team. The majority (64%) of students engaging in translational research were undergraduates. Racial and ethnic diversity was evident with 16% Latinx, 14% Black/African American, 12% Asian, 10% two or more races, and 4% Native American or Native Hawaiian. A large proportion (42%) of students indicated that they were first-generation college students, while 24% indicated they had a disability. Five themes emerged from student feedback about their involvement in the experiential learning course: the value of translational research, development of research skills, collaboration, practice development, and value for community partners.

Conclusions for Practice
Through an MCH translational research team, we have established a pathway to enhance diversity among the MCH workforce which will increase recruitment and retention of underrepresented groups, and ultimately improve MCH research and practice.
Anchoring in truncation: A typological analysis
Authors: Alber Birgit , Arndt-Lappe Sabine 
Published: January 2022

AbstractAnalyses of truncation patterns (e.g. Rob, from Robert) have traditionally focused on the templatic shape of such forms, while less attention has been paid to the question of which parts of the base word may survive in the truncatum. This feature of truncation, usually referred to as ‘anchoring,’ is investigated in the present paper. On the empirical basis of the generalizations emerging from an extensive database of truncation patterns in the world’s languages, a formal typology of anchoring in the framework of Optimality Theory is constructed and its defining ranking conditions (its ‘typological properties’) are extracted. The typological properties reveal the grammatical forces shaping the various classes of truncation patterns. They show that anchoring constraints must indeed form an integral part of any model of morphological truncation since—in interaction with templatic size-restrictor constraints—they determine whether truncation occurs at all and whether output forms vary in size or are of a fixed templatic shape. A thorough analysis of anchoring thus also provides evidence for templatic shapes emerging from constraint interaction and against an approach in terms of fixed, language-specific templates.
Unrestricted quantification and ranges of significance
Authors: Schindler Thomas 
Published: January 2022

AbstractCall a quantifier ‘unrestricted’ if it ranges over absolutely all objects. Arguably, unrestricted quantification is often presupposed in philosophical inquiry. However, developing a semantic theory that vindicates unrestricted quantification proves rather difficult, at least as long as we formulate our semantic theory within a classical first-order language. It has been argued that using a type theory as framework for our semantic theory provides a resolution of this problem, at least if a broadly Fregean interpretation of type theory is assumed. However, the intelligibility of this interpretation has been questioned. In this paper I introduce a type-free theory of properties that can also be used to vindicate unrestricted quantification. This alternative emerges very naturally by reflecting on the features on which the type-theoretic solution of the problem of unrestricted quantification relies. Although this alternative theory is formulated in a non-classical logic, it preserves the deductive strength of classical strict type theory in a natural way. The ideas developed in this paper make crucial use of Russell’s notion of range of significance.
Fields with no recent legume cultivation have sufficient nitrogen-fixing rhizobia for crops of faba bean (Vicia faba L.)
Authors: Maluk Marta , Ferrando-Molina Francesc , Lopez del Egido Laura , Langarica-Fuentes Adrian , Yohannes Genet Gebre , Young Mark W. , Martin Peter , Gantlett Richard , Kenicer Greg , Hawes Cathy , Begg Graham S. , Quilliam Richard S. , Squire Geoffrey R. , Young J. Peter W. , Iannetta Pietro P. M. , James Euan K. 
Published: January 2022

Abstract
Purpose
(1) To assess the biological N fixation (BNF) potential of varieties of faba bean (Vicia faba L.) cropped with or without compost in an experimental field-scale rotation with no recent history of legumes, (2) to enumerate soil populations of Rhizobium leguminosarum sv. viciae (Rlv), and to genetically characterize the nodulating Rlv strains, (3) compare BNF with other sites in Britain.

Methods
BNF was evaluated from 2012 to 2015 using 15 N natural abundance. Treatments were either PK fertilizer or compost. Soil rhizobial populations were determined using qPCR, the symbiotic rhizobia genotyped (16 S rRNA, nodA and nodD genes), and their BNF capacity assessed ex situ. The reliance of legumes on BNF at other British sites was estimated in a single season, and their nodulating symbionts examined.

Results
Faba bean obtained most of its N through BNF (>80%) regardless of variety or year. N-accumulation by cvs Babylon and Boxer increased with compost treatment in 2014/2015. Rhizobial populations were c. 105-106Rlv cells g−1 soil regardless of field or treatment. 157 Rlv microsymbionts grouped into two large nodAD clades; one mainly from V. faba, and the other from various legumes. All isolates nodulated, and some performed better than commercial inoculant strains.

Conclusions
Faba bean can provide most of its nitrogen through BNF and leave economically valuable residual N for subsequent crops. Recent legume cropping in northern Europe is not essential for effective nodulation: rhizobia may persist in a range of farmland locations. Nevertheless, there is the potential to apply elite rhizobial strains as inoculants in some soils.
Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free
Authors: Remonatto Daniela , Oliveira J. Vladimir , Guisan J. Manuel , Oliveira Débora , Ninow Jorge , Fernandez-Lorente Gloria 
Published: January 2022

AbstractLipases are an important group of biocatalysts for many industrial applications. Two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 was immobilized on four different hydrophobic supports: Lewatit-DVB, Purolite-DVB, Sepabeads-C18, and Purolite-C18. The performance of immobilized lipases was investigated in the transesterification of sunflower oil solvent-free in an anhydrous medium. Interesting results were obtained for both lipases and the four supports, but with Sepabeads support the lipases Eversa showed high catalytic activity. However, the more stable and efficient derivative was Eversa® Transform immobilized on Sepabeads C-18. A 98 wt% of ethyl ester of fatty acid (FAEE) was obtained, in 3 h at 40ºC, ethanol/sunflower oil molar ratio of 3:1 and a 10 wt% of the immobilized biocatalyst. After 6 reaction cycles, the immobilized biocatalyst preserved 70 wt% of activity. Both lipases immobilized in Sepabeads C-18 were highly active and stable in the presence of ethanol. The immobilization of Eversa Transform and Eversa Transform 2.0 in hydrophobic supports described in this study appears to be a promising alternative to the immobilization and application of these news lipases still unexplored.
Genotyping-by-sequencing based SNP discovery in a non-model rodent, the endangered hazel dormouse
Authors: Leyhausen Johanna , Cocchiararo Berardino , Nowak Carsten , Ansorge Hermann , Bertolino Sandro , Büchner Sven , Fietz Joanna , Foppen Ruud , Juškaitis Rimvydas , La Haye Maurice , Lang Johannes , Michaux Johan , Verbeylen Goedele , von Thaden Alina , Mueller Sarah A. 
Published: January 2022

AbstractThe hazel dormouse Muscardinus avellanarius presents an exemplary non-model species that is both locally threatened and whose genetic status is not fully understood owing to insufficient resolution of the currently available molecular tools. We performed normalized Genotyping-by-Sequencing (nGBS) on 48 hazel dormouse samples collected across the species European distribution, aiming at discovering useful single nucleotide polymorphism (SNP) markers for the assessment of population structure and genomic diversity. The analyses of > 24,000 SNPs showed a high divergence between the Eastern and Western lineage of the species with high rates of SNP allele fixation, consistent with previous studies suggesting the divergence of lineages occurred over 2 mya. These results indicate that investigating inter-lineage as well as within-lineage genetic composition will be a conclusive approach for identifying conservation strategies in the future. Results presented here indicate the highest genetic divergence in the Italian and Lithuanian populations. We document how nGBS allows the discovery of SNPs that can characterize patterns of genetic variation at multiple spatial scales in a non-model organism. We document how nGBS allows the discovery of SNPs that can characterize patterns of genetic variation at multiple spatial scales in a non-model organism, potentially informing monitoring and conservation strategies.
Emerging Synergies in Innovation Systems: Creative Industries in Central Europe
Authors: Szakálné Kanó Izabella , Vas Zsófia , Klasová Slávka 
Published: January 2022

AbstractThe window of opportunity has opened for the creative industries in Central Europe in the decades following the fall of the communist regime and the transition to a decentralised market economy. Creative industries with symbolic knowledge base are crucial for regional resilience, and regional synergies established by these economic activities highly influence the long-term ability of innovation systems to develop. The question is whether creative industries have started to grow and can contribute to the development of post-socialist knowledge-based economies in Central Europe, if so, at what territorial level. By distinguishing three dimensions (geography, technology, organisation) that operate in innovation systems and by measuring their interactions using entropy statistics in two post-socialist countries, Slovakia and Hungary, the paper reveals that the most significant part of the synergy in creative industries emerges at the local level of innovation systems. However, benefits are realised not only locally but also globally due to the deterritorialised nature of the end-products and their integration into other industrial products on the global market.
Genetic identifications challenge our assumptions of physical development and mother–calf associations and separation times: a case study of the North Atlantic right whale (Eubalaena glacialis)
Authors: Hamilton Philip K. , Frasier Brenna A. , Conger Lisa A. , George R. Clay , Jackson Katharine A. , Frasier Timothy R. 
Published: January 2022

AbstractWhile photo-identification is an effective tool to monitor individuals in wild populations, it has limitations. Specifically, it cannot be applied to very young animals before their identifying features have stabilized or to dead, decomposed animals. These shortfalls leave gaps in our understanding of survival, parentage, age structure, physical development, and behavioral variability. Here we report on 13 case studies of North Atlantic right whale, Eubalaena glacialis, calves that required genetics to track their life history data. These case studies revealed unexpected variations in mother–calf associations and separation times, as well as calf physical development. Prior to this study, calves were assumed to have died if their mothers were always alone on the feeding ground in the calf’s birth year. Using genetics and photo-identification, four such calves were discovered to be alive; two of the four possibly weaned earlier than expected at 7.5–8.0 months. To put these early separations in context, photo-identification data were queried and revealed that mothers and calves are seen apart from each other on the feeding grounds in 10–40% of all spring/summer sightings; previously, there were no published data on how often pairs are seen apart in the calf’s birth year. Two dead whales initially logged as calves of the year were discovered to be juveniles, thus allowing skewed survival estimates for calves of the year to be corrected. Genetically sampling animals early in their lives before they disperse or separate from their mothers provides an important means of individual identification at a time when photo-identification is not reliable.
Institutional quality and resource-based economic sustainability: the mediation effects of resource governance
Authors: Kansheba Jonathan Mukiza Peter , Marobhe Mutaju Isack 
Published: January 2022

AbstractThe discovery of extractive resources is associated with multiple opportunities and unbridled optimism on achieving socio-economic development for many countries. However, the question how the host governments meet expectations of indigenous people by ensuring an ideal resource-based economic sustainability (RES) has been receiving less research attention. Using the global panel dataset of 80 resource-endowed economies from 2010 to 2017, we postulate and empirically examine the mediating effect of the resource governance (RESOGV) on the relationship between institutional quality (IQ) and RES. The ANOVA and post hoc ANOVA results revealed significant disparities in terms of IQ, RESOGV, and RES among countries with different levels of economic development, regions, and sector (mining or petroleum). Moreover, the fixed effects (FE) and ordinary least square (OLS) results confirmed that IQ marginally influenced RES. However, the influence was more pronounced when it was partially (but complementarily) mediated by the RESOGV. The paper, therefore, stresses the importance of host governments in improving the quality of their institutions. This will ultimately help enhancing their RESOGV capabilities for attaining economic, social and environmental sustainability.
‘Not birth, marriage or death, but gastrulation’: the life of a quotation in biology
Authors: Hopwood Nick 
Published: January 2022

AbstractThis history of a statement attributed to the developmental biologist Lewis Wolpert exemplifies the making and uses of quotations in recent science. Wolpert's dictum, ‘It is not birth, marriage or death, but gastrulation which is truly the most important time in your life’, was produced in a series of international shifts of medium and scale. It originated in his vivid declaration in conversation with a non-specialist at a workshop dinner, gained its canonical form in a colleague's monograph, and was amplified as a quotation on a poster derived from an undergraduate project. Although it drew on Wolpert's authority and he accepted his authorship, it thus represents a collective sifting of earlier claims for the significance of prenatal existence through the values of 1980s developmental biology. Juxtaposing a technical term with major life events has let teachers engage students, and researchers entice journalists, while sharing an in-joke that came to mark community identity. Serious applications include arguing for an extension of the fourteen-day limit on human-embryo research. On this evidence, quotations have been kept busy addressing every audience of specialized knowledge.
Experiencing Time in the Early English East India Company
Authors: Williams Mark R. F. 
Published: January 2022

AbstractThe structuring of time in the early modern period has traditionally been associated with a broad, European-led shift towards ‘accuracy’, and with it connotations of ‘modernity’. Yet, a fuller examination of the temporal world of early modern merchants challenges such a teleology. Taking as its focus the English East India Company (EIC), this article situates concerns for accuracy in the management of time (and with it profit) within a broader spectrum of temporal influences, including seasonal, indigenous, and embodied time. It draws upon the experiences of the merchant Isaac Lawrence (1639–79), whose trade began around the Mediterranean Sea but ended in the service of the EIC in Persia. Like many of his contemporaries in the Company, Lawrence died relatively young and in obscure circumstances; however, the survival of his personal papers through his brother William Lawrence affords vital insights into how time was observed, measured, and felt within mobile early modern lives. Read alongside Company records, Lawrence's experience makes clear the necessity of reading subjective temporalities into historical understandings of time within global frameworks.
Exploring the use of games in palliative care: A scoping review
Authors: Fernandes Carla Sílvia , Vale Maria Belém , Lourenço Marisa 
Published: January 2022

AbstractObjectiveThere has been increasing recognition of the potential of games in health; however, knowledge of their application in palliative care is lacking. Therefore, this study aimed to identify and map the available evidence on the use of games in palliative care, analyzing how research has been conducted on this topic and identifying gaps in knowledge.MethodA scoping review was carried out. The literature search was conducted using the respective descriptors and search syntax appropriate to each of the databases searched. The review included all study types with no time limits.ResultsOf the 685 articles initially identified, 53 were included for final analysis. Several different game types were identified, with the majority of studies using role-play (n= 29) and card games (n= 17). The games analyzed were essentially aimed at empowering patients (n= 14), and in some cases, extended to families or caregivers, as well as to medical and nursing students. The analysis of the articles in this review resulted in two major themes: Role-playing for training in palliative care and card games to discuss end-of-life care.Significance of resultsGames allow space for the expression of emotions and promote creativity. They can be applied both in a training context, to enable health professionals to develop essential skills in palliative care, and for patients, families, and caregivers, allowing them to talk about serious things while playing.
The social and spiritual dimensions of parental experiences on end-of-life care of their child with cancer: A narrative review
Authors: Job Maria Katharina , Schumacher Petra , Müller Gerhard , Kreyer Christiane 
Published: January 2022

AbstractObjectiveHaving a child with cancer is a burdensome experience for parents. Nurses need to better understand beliefs, hopes, and values of parents to holistically support them, which may have an impact on grief and depression. Thus, the aim of this paper was to highlight the social and spiritual dimensions of parental experiences on end-of-life care of their child with cancer.MethodA narrative literature review was conducted by systematically searching databases (CINAHL Complete, Academic Search Elite, MEDLINE) for relevant literature. The contents of included studies were critically appraised regarding their methodological quality.ResultsEleven studies were included. In terms of the social dimension, three categories emerged that are important for parents: Being a Parent, Being in Relationship with the Child, and Being in Relationship with Health Professionals. Parents tried to maintain normality and joy for their child, but also kept control over the situation. They wanted to protect their child from suffering but also felt the need to talk to them about dying. Parents had ambivalent experiences with helpful but also burdensome staff. The spiritual dimension comprised two main categories, Hope and Faith and a Unique Bond. Hope was found to be an important source of strength, while Faith was only mentioned by some parents. For parents the emotional connection with their child constituted a source of meaning as well as a foundation, on which they based their decisions on.Significance of resultsA therapeutic relationship and a supportive environment can be established by healthcare professionals by noticing, encouraging the unique bond between parents and their children, as well as by reflecting and addressing the parents’ challenging situation.