bluuebunny/crossref_metadata_2025_split huggingface dataset:
The use of Method Information Index (MII) to monitor the amount of information given to women users of modern contraceptives in Indonesia: results from an analysis of the 2007, 2012 and 2017 demographic and health surveys
Authors: Budiharsana Meiwita P. , Wahyuningsih Wiji , Heywood Peter 
Published: December 2022

Abstract
Background
The use of Method Information Index (MII) indicates whether women contraceptive users receive adequate information about all available contraceptive methods, side effects of the methods, and how to deal with the side effects if experienced—at method initiation.

Objective
This study aims to investigate the level of MII scores or the amount of information received by married women users of five modern contraceptives at the time of initiation and changes of its determinants based on the Indonesian Demographic and Health data between 2007 and 2017.

Methods
Data of married women who used most common five modern contraceptive methods (the pill, injectables, implants, IUD, and female sterilization), comprised of a total unweighted sample of 35,412 users out of the 32,895; 45,607 and 49,627 women aged 15–49 in the 2007, 2012, and 2017 Indonesian Demographic and Health Survey (IDHS), respectively. The Method Information Index (MII) scores were calculated based on responses to three questions (whether women were told about method‐specific side effects, advised what to do if they experienced them, and informed about other available methods). Multivariable logistic regressions with ‘time’ as an interaction variable were used to assess the influence of time upon the MII scores and its determinants.

Results
The MII scores were 23.84% in 2007, 24.60% in 2012 and 28.65% in 2017. Obviously, over 70% of reproductive-age women contraceptive users were not receiving complete information about modern contraceptives at the time of initiation. After 5 years (2012), only living in the Java Bali region (AOR = 1.34, 95% CI 1.09–1.66) compared to living in other islands, and currently using injectables (AOR = 1.43, 95% CI 1.10–1.87) and currently using implants (AOR = 1.68, 95% CI 1.07–2.63) compared to currently using pills had significantly higher odds of receiving MII information. After 10 years (2017), only one variable (the ‘richest’ in the wealth quintile category (AOR = 0.70, 95% CI 0.50–0.99) compared to the ‘poorest’) still showed a significant association with receipt of complete MII information.

Conclusions
Despite the fact that the MII scores increased gradually across the years, interaction with ‘survey time’ showed that the likelihood of receiving complete MII information was not statistically different in the 5 years (2007–2012) and in the 10 years (2007–2017) period from the reference category in 2007. The authors recommend use of the MII score as an objective measure to evaluate access to MII essential information and to monitor an increase in the informed population in Indonesia.
Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A
Authors: Ma Zhongyuan , Lv Jianliang , Zhang Zhongwang , Pan Li 
Published: December 2022

Abstract
Background
Senecavirus A (SVA) is a pathogen that has recently caused porcine idiopathic vesicular disease (PIVD). The clinical signs are similar to those of foot-and-mouth disease, porcine vesicular disease, and vesicular stomatitis. Therefore, identification of SVA as a cause of PIVD is important to eliminate this emerging pathogen.

Methods
In this study, an indirect ELISA based on the VP2 epitope (VP2-epitp-ELISA) was developed to detect antibodies directed against SVA.

Results
A novel linear epitope (271GLRNRFTTGTDEEQ284) was first identified at the C-terminus of the VP2 protein by epitope mapping. The diagnostic performance of VP2-epitp-ELISA was estimated by testing a panel of known background sera from swine. Under the optimum test conditions, when the cutoff value was 37%, the diagnostic sensitivity (Dn) and diagnostic specificity (Dp) of the assay were 91.13% and 91.17%, respectively. The accuracy of VP2-epitp-ELISA was validated and further compared with that of commercial diagnostic kits. The diagnostic results showed that VP2-epitp-ELISA did not cross-react with serum positive for other idiopathic vesicular diseases and had a concordance rate of 90.41% with the Swinecheck® SVA bELISA.

Conclusions
These results indicate that VP2-epitp-ELISA is suitable for specific detection of antibodies against SVA in swine.
Genetic characterization and pathogenicity of a Eurasian avian-like H1N1 swine influenza reassortant virus
Authors: Zhu Hechao , Li Xiangmin , Chen Huanchun , Qian Ping 
Published: December 2022

Abstract
Background
Swine influenza viruses (SIV), considered the “mixing vessels” of influenza viruses, posed a significant threat to global health systems and are dangerous pathogens. Eurasian avian-like H1N1(EA-H1N1) viruses have become predominant in swine populations in China since 2016.

Methods
Lung tissue samples were obtained from pregnant sows with miscarriage and respiratory disease in Heilongjiang province, and pathogens were detected by Next-generation sequencing (NGS) and PCR. The nucleic acid of isolates was extracted to detect SIV by RT-PCR. Then, SIV-positive samples were inoculated into embryonated chicken eggs. After successive generations, the isolates were identified by RT-PCR, IFA, WB and TEM. The genetic evolution and pathogenicity to mice of A/swine/Heilongjiang/GN/2020 were analyzed.

Results
The major pathogens were influenza virus (31%), Simbu orthobunyavirus (15%) and Jingmen tick virus (8%) by NGS, while the pathogen that can cause miscarriage and respiratory disease was influenza virus. The SIV(A/swine/Heilongjiang/GN/2020) with hemagglutination activity was isolated from lung samples and was successfully identified by RT-PCR, IFA, WB and TEM. Homology and phylogenetic analysis showed that A/swine/Heilongjiang/GN/2020 is most closely related to A/swine/Henan/SN/10/2018 and belonged to EA-H1N1. Pathogenicity in mice showed that the EA-H1N1 could cause lethal or exhibit extrapulmonary virus spread and cause severe damage to respiratory tracts effectively proliferating in lung and trachea.

Conclusion
A/swine/Heilongjiang/GN/2020 (EA-H1N1) virus was isolated from pregnant sows with miscarriage and respiratory disease in Heilongjiang province, China. Clinical signs associated with influenza infection were observed during 14 days with A/swine/Heilongjiang/GN/2020 infected mice. These data suggest that A/swine/Heilongjiang/GN/2020 (EA-H1N1) had high pathogenicity and could be systemic spread in mice.
Prehospital monitoring of cerebral circulation during out of hospital cardiac arrest ? A feasibility study
Authors: Henningsson Anna , Lannemyr Lukas , Angerås Oskar , Björås Joakim , Bergh Niklas , Herlitz Johan , Redfors Bengt , Lundgren Peter 
Published: December 2022

Abstract
Background
About two-thirds of the in-hospital deaths after out-of-hospital cardiac arrests (OHCA) are a consequence of anoxic brain injuries, which are due to hypoperfusion of the brain during the cardiac arrests. Being able to monitor cerebral perfusion during cardiopulmonary resuscitation (CPR) is desirable to evaluate the effectiveness of the CPR and to guide further decision making and prognostication.

Methods
Two different devices were used to measure regional cerebral oxygen saturation (rSO2): INVOS™ 5100 (Medtronic, Minneapolis, MN, USA) and Root® O3 (Masimo Corporation, Irvine, CA, USA). At the scene of the OHCA, advanced life support (ALS) was immediately initiated by the Emergency Medical Services (EMS) personnel. Sensors for measuring rSO2 were applied at the scene or during transportation to the hospital. rSO2 values were documented manually together with ETCO2 (end tidal carbon dioxide) on a worksheet specially designed for this study. The study worksheet also included a questionnaire for the EMS personnel with one statement on usability regarding potential interference with ALS.

Results
Twenty-seven patients were included in the statistical analyses. In the INVOS™5100 group (n = 13), the mean rSO2 was 54% (95% CI 40.3–67.7) for patients achieving a return of spontaneous circulation (ROSC) and 28% (95% CI 12.3–43.7) for patients not achieving ROSC (p = 0.04). In the Root® O3 group (n = 14), the mean rSO2 was 50% (95% CI 46.5–53.5) and 41% (95% CI 36.3–45.7) (p = 0.02) for ROSC and no ROSC, respectively. ETCO2 values were not statistically different between the groups. The EMS personnel graded the statement of interference with ALS to a median of 2 (IQR 1–6) on a 10-point Numerical Rating Scale.

Conclusion
Our results suggest that both INVOS™5100 and ROOT® O3 can distinguish between ROSC and no ROSC in OHCA, and both could be used in the pre-hospital setting and during transport with minimal interference with ALS.
'Fight the parasite': raising awareness of cystic echinococcosis in primary school children in endemic countries
Authors: Porcu Francesca , Cantacessi Cinzia , Dessì Giorgia , Sini Maria Francesca , Ahmed Fahad , Cavallo Lia , Nonnis Francesca , Gibson Katherine , Varcasia Cecilia , Joanny Gaelle , Scala Antonio , Tamponi Claudia , Varcasia Antonio 
Published: December 2022

Abstract
Background
Cystic echinococcosis (CE) is a widespread zoonosis and a significant economic concern and cause of morbidity in humans. A scarcity of education on the sources of CE infection and containment measures is considered to be a key factor responsible for persistent transmission within communities. Recently, edutainment approaches have captured the attention of health education (HE) professionals due to the benefits of integrating cognitive and emotional learning processes.

Methods
A study was carried out in Sardinia, Italy, between 2020 and 2022, amid the SARS-Covid-19 pandemic. The project, designed to involve primary school children (via remote or face-to-face learning depending on the evolving Covid-19 containment measures) consisted of four distinct phases: (i) creation of material for school children and teachers focused on cystic echinococcosis; (ii) pre-intervention evaluation of CE knowledge (i.e. True False Don’t Know [TFD] pre-intervention questionnaire based on CE-related knowledge and practices); (iii) edutainment activity (e.g. interactive lessons enhanced by the comic booklet and the “Fight the parasite” cartoon video, hands-on educational activities and drawing activities on CE); and (iv) post-intervention evaluation of CE knowledge (via TFD post-intervention questionnaire [same questionnaire as used for the pre-intervention assessment] on CE-related knowledge and practices) and on-site edutainment tour in primary schools taking part to the project.

Results
The percentage of correct answers increased from 65% for the questionnaire administered pre-intervention to 87.9% for the same questionnaire administered post-intervention (χ2 = 648.12, df = 1, P < 0.0001), while the percentage of uncertain answers (i.e. ‘I don’t know’) decreased from 23% pre-intervention to 5% post-intervention (χ2 = 603.44, df = 1, P < 0.0001). These differences indicate a significantly enhanced understanding of CE among participating school children after the intervention.

Conclusions
The results of the present survey indicate that the use of digital educational tools, the use of video animations as a model for science communication, as well as other participatory teaching methods, enabled children to retain key knowledge of the routes of CE transmission and ways to prevent it.

Graphical abstract
Risk factors for shoulder injuries in handball: systematic review
Authors: Hadjisavvas Stelios , Efstathiou Michalis A. , Malliou Vivian , Giannaki Christoforos D. , Stefanakis Manos 
Published: December 2022

AbstractBackgroundHandball is a sport with a high number of severe injuries.The shoulder is one of the most commonly injured joints, with an average prevalence of 17–41%.ObjectiveThe primary aim is to identify the most significant risk factors related to shoulder injuries in handball. The secondary aim is to propose recommendations based on the available evidence concerning potential injury prevention strategies.MethodsSystematic searches of PubMed, MEDLINE, CINAHL, Proquest, SPORTDiscus, Web of Science, EMBASE, and Scopus were undertaken for peer reviewed articles published between 15 July 1995 and 15 July 2019. The same search strategy was repeated on 24 April 2022. Prospective cohort studies, written in English and published in a journal with a peer-review process aiming to investigate at least one modifiable or/and a non-modifiable potential risk factor for shoulder injuries, specifically in handball players, were included. Only papers published after 1995 were included. The methodological quality of the eligible studies was assessed using the modified version of the Downs and Black Checklist. The Best Evidence Synthesis (BES) approach was used for synthesizing and reporting the results.Results1849 studies were identified, of which 8 were included. A total of 2536 (males = 1354, females = 1182) participants of which 2522 were handball athletes, were included. Four of the eight studies were rated as high methodological quality studies (> 85%) while the rest were rated as medium (50–85%). The risk factors for shoulder injuries in handball identified in the studies were strength imbalances (n = 6), glenohumeral range of motion (ROM) imbalances (n = 5), scapular dyskinesis (n = 5), incorrect dosage of training load (n = 2), previous injury (n = 1), sex (n = 2), player’s position, school grade, playing level (n = 1), altered shoulder joint position sense (n = 1).ConclusionOverall, from all the risk factors evaluated, there was strong evidence that the weakness of the shoulder external rotator muscles and the female sex increase the probability of shoulder injury in handball athletes. Nevertheless, the evidence for the other risk factors was moderate due to the methodological quality and the limited number of studies.Protocol registration:PROSPERO ID: CRD42020138233.
Dynamic transcriptome analysis reveals the gene network of gonadal development from the early history life stages in dwarf surfclam Mulinia lateralis
Authors: Li Yajuan , Liu Liangjie , Zhang Lijing , Wei Huilan , Wu Shaoxuan , Liu Tian , Shu Ya , Yang Yaxin , Yang Zujing , Wang Shi , Bao Zhenmin , Zhang Lingling 
Published: December 2022

AbstractBackgroundGonadal development is driven by a complex genetic cascade in vertebrates. However, related information remains limited in molluscs owing to the long generation time and the difficulty in maintaining whole life cycle in the lab. The dwarf surfclamMulinia lateralisis considered an ideal bivalve model due to the short generation time and ease to breed in the lab.ResultsTo gain a comprehensive understanding of gonadal development inM. lateralis, we conducted a combined morphological and molecular analysis on the gonads of 30 to 60 dpf. Morphological analysis showed that gonad formation and sex differentiation occur at 35 and 40–45 dpf, respectively; then the gonads go through gametogenic cycle. Gene co-expression network analysis on 40 transcriptomes of 35–60 dpf gonads identifies seven gonadal development-related modules, including two gonad-forming modules (M6, M7), three sex-specific modules (M14, M12, M11), and two sexually shared modules (M15, M13). The modules participate in different biological processes, such as cell communication, glycan biosynthesis, cell cycle, and ribosome biogenesis. Several hub transcription factors includingSOX2,FOXZ,HSFY, FOXL2andHES1are identified. The expression of top hub genes from sex-specific modules suggests molecular sex differentiation (35 dpf) occurs earlier than morphological sex differentiation (40–45 dpf).ConclusionThis study provides a deep insight into the molecular basis of gonad formation, sex differentiation and gametogenesis inM. lateralis, which will contribute to a comprehensive understanding of the reproductive regulation network in molluscs.
The COVID-19 pandemic and food security in low- and middle-income countries: a review
Authors: Bloem Jeffrey R. , Farris Jarrad 
Published: December 2022

AbstractWe review findings from the emerging microeconomic literature on observed changes in food insecurity associated with the COVID-19 pandemic. To do so, we focus our review on studies in low- and middle-income countries that include household survey data measuring food insecurity collected both before and after the onset of the COVID-19 pandemic. We first focus on several studies—seven from countries in Sub-Saharan Africa and one from India—that estimate immediate changes in food insecurity associated with the COVID-19 pandemic. Next, we review subsequent analysis studying longer term changes in food insecurity associated with the COVID-19 pandemic. This review, therefore, complements existing macroeconomic projections of food insecurity based on expected changes in income and prices.
Population structure of an Antarctic aquatic cyanobacterium
Authors: Panwar Pratibha , Williams Timothy J. , Allen Michelle A. , Cavicchioli Ricardo 
Published: December 2022

AbstractBackgroundAce Lake is a marine-derived, stratified lake in the Vestfold Hills of East Antarctica with an upper oxic and lower anoxic zone. Cyanobacteria are known to reside throughout the water column. ASynechococcus-like species becomes the most abundant member in the upper sunlit waters during summer while persisting annually even in the absence of sunlight and at depth in the anoxic zone. Here, we analysed ~ 300 Gb of Ace Lake metagenome data including 59Synechococcus-like metagenome-assembled genomes (MAGs) to determine depth-related variation in cyanobacterial population structure. Metagenome data were also analysed to investigate viruses associated with this cyanobacterium and the host’s capacity to defend against or evade viruses.ResultsA singleSynechococcus-like species was found to exist in Ace Lake,CandidatusRegnicoccus frigidus sp. nov., consisting of one phylotype more abundant in the oxic zone and a second phylotype prevalent in the oxic-anoxic interface and surrounding depths. An important aspect of genomic variation pertained to nitrogen utilisation, with the capacity to perform cyanide assimilation and asparagine synthesis reflecting the depth distribution of available sources of nitrogen. Both specialist (host specific) and generalist (broad host range) viruses were identified with a predicted ability to infectCa.Regnicoccus frigidus. Host-virus interactions were characterised by a depth-dependent distribution of virus type (e.g. highest abundance of specialist viruses in the oxic zone) and host phylotype capacity to defend against (e.g. restriction-modification, retron and BREX systems) and evade viruses (cell surface proteins and cell wall biosynthesis and modification enzymes).ConclusionIn Ace Lake, specific environmental factors such as the seasonal availability of sunlight affects microbial abundances and the associated processes that the microbial community performs. Here, we find that the population structure forCa.Regnicoccus frigidus has evolved differently to the other dominant phototroph in the lake,CandidatusChlorobium antarcticum. The geography (i.e. Antarctica), limnology (e.g. stratification) and abiotic (e.g. sunlight) and biotic (e.g. microbial interactions) factors determine the types of niches that develop in the lake. While the lake community has become increasingly well studied, metagenome-based studies are revealing that niche adaptation can take many paths; these paths need to be determined in order to make reasonable predictions about the consequences of future ecosystem perturbations.
The bridge-like lipid transfer protein (BLTP) gene group: introducing new nomenclature based on structural homology indicating shared function
Authors: Braschi Bryony , Bruford Elspeth A. , Cavanagh Amy T. , Neuman Sarah D. , Bashirullah Arash 
Published: December 2022

AbstractThe HUGO Gene Nomenclature Committee assigns unique symbols and names to human genes. The use of approved nomenclature enables effective communication between researchers, and there are multiple examples of how the usage of unapproved alias symbols can lead to confusion. We discuss here a recent nomenclature update (May 2022) for a set of genes that encode proteins with a shared repeating β-groove domain. Some of the proteins encoded by genes in this group have already been shown to function as lipid transporters. By working with researchers in the field, we have been able to introduce a new root symbol (BLTP, which stands for “bridge-like lipid transfer protein”) for this domain-based gene group. This new nomenclature not only reflects the shared domain in these proteins, but also takes into consideration the mounting evidence of a shared lipid transport function.
Analog field-scale acoustic study of volcanic eruption directivity using a tiltable liquid nitrogen-charged water cannon
Authors: Jolly Arthur D. , Kennedy Ben , Matoza Robin S. , Iezzi Alexandra M. , Christenson Bruce , Johnson Richard , Sork Amilea , Fee David 
Published: December 2022

AbstractLaterally directed explosive eruptions are responsible for multiple fatalities over the past decade and are an increasingly important volcanology problem. To understand the energy dynamics for these events, we collected field-scale explosion data from nine acoustic sensors surrounding a tiltable cannon as part of an exploratory experimental design. For each cannon discharge, the blast direction was varied systematically at 0°, 12°, and 24° from vertical, capturing acoustic wavefield directivity related to the tilt angle. While each event was similar in energy discharge potential, the resulting acoustic signal features were variable event-to-event, producing non-repetitious waveforms and spectra. Systematic features were observed in a subset of individual events for vertical and lateral discharges. For vertical discharges, the acoustic energy had a uniform radiation pattern. The lateral discharges showed an asymmetric radiation pattern with higher frequencies in the direction of the blast and depletion of those frequencies behind the cannon. Results suggest that, in natural volcanic systems, near-field blast directionality may be elucidated from acoustic sensors in absence of visual data, with implications for volcano monitoring and hazard assessment.
Graphical Abstract