bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenology effects on productivity and hatching-asynchrony of American kestrels (Falco sparverius) across a continent
Authors: Callery Kathleen R. , Schulwitz Sarah E. , Hunt Anjolene R. , Winiarski Jason M. , McClure Christopher J. W. , Fischer Richard A. , Heath Julie A. 
Published: January 2022

AbstractClimate-driven advances in spring can result in phenological mismatch between brood rearing and prey availability and consequently cause decreased productivity in birds. How consequences of mismatch vary across species’ ranges, and how individual behavior can mitigate mismatch effects is less studied. We quantified the relationship between phenological mismatch, productivity, and behavioral adaptations of American kestrels (Falco sparverius) across their breeding range in the United States and southern Canada. We obtained phenology and productivity data using nest observations from long term nest box monitoring, remote trail cameras, and community-scientist based programs. We collected data on parental incubation behavior and hatch asynchrony using trail cameras in nest boxes. Kestrels that laid eggs after the start of spring had higher rates of nest failure and fewer nestlings than earlier nesters, and effects of mismatch on productivity were most severe in the Northeast. In contrast, kestrels in the Southwest experienced a more gradual decline in productivity with seasonal mismatch. We attribute the effect of location to the growing season and temporal nesting windows (duration of nesting season). Specifically, resource availability in the Northeast is narrow and highly peaked during the breeding season, potentially resulting in shorter nesting windows. Conversely, resource curves may be more prolonged and dampened in the Southwest, and growing seasons are becoming longer with climate change, potentially resulting in longer nesting windows. We found that the onset of male incubation was negatively associated with lay date. Males from breeding pairs that laid eggs after the start of spring began incubation sooner than males from breeding pairs that laid before the start of spring. Early-onset male incubation was positively associated with hatching asynchrony, creating increased age variation in developing young. In sum, we demonstrate that American kestrels are vulnerable to phenological mismatch, and that this vulnerability varies across space. Northeastern populations could be more vulnerable to mismatch consequences, which may be one factor contributing to declines of kestrels in this region. Also, we demonstrate early onset of incubation as a potential adaptive behavior to advance average hatch date and spread out offspring demands, but it is unknown how impactful this will be in mitigating the fitness consequences of phenology mismatch.HighlightsClimate-driven phenological mismatch is a growing conservation concern.We studied phenology and productivity of an avian predator across North America.American kestrels nesting after the start of spring had lower productivity.Later nesting kestrels altered incubation to create hatching asynchrony.Mismatch effects were strongest in the Northeast and may contribute to population declines.
Integrative analysis of relative abundance data and presence-absence data of the microbiome using the LDM
Authors: Zhu Zhengyi , Satten Glen A. , Hu Yi-Juan 
Published: January 2022

AbstractSummaryWe previously developed LDM for testing hypotheses about the microbiome that performs the test at both the community level and the individual taxon level. LDM can be applied to relative abundance data and presence-absence data separately, which work well when associated taxa are abundant and rare, respectively. Here we propose an omnibus test based on LDM that allows simultaneous consideration of data at different scales, thus offering optimal power across scenarios with different association mechanisms. The omnibus test is available for the wide range of data types and analyses that are supported by LDM.Availability and ImplementationThe omnibus test has been added to the R package LDM, which is available on GitHub at https://github.com/yijuanhu/LDM.Contactyijuan.hu@emory.eduSupplementary informationSupplementary data are available at Bioinformatics online.
Cross-species permissivity: structure of a goat adeno-associated virus and its complex with the human receptor, AAVR
Authors: Large Edward E. , Silveria Mark A. , White Tommi A. , Chapman Michael S. 
Published: January 2022

ABSTRACTAdeno-associated virus (AAV) is a small ssDNA satellite virus of high interest (in recombinant form) as a safe and effective gene therapy vector. AAV’s human cell entry receptor (AAVR) contains Polycystic Kidney Disease (PKD) domains bound by AAV. Seeking understanding of the spectrum of interactions, goat AAVGo.1 is investigated, because its host is the species most distant from human with reciprocal cross-species cell susceptibility. The structure of AAVGo.1, solved by cryo-EM to 2.9 Å resolution, is most similar to AAV5. Through ELISA studies, it is shown that AAVGo.1 binds to human AAVR (huAAVR) more strongly than do AAV2 or AAV5, and that it joins AAV5 in a class that binds exclusively to PKD domain 1 (PKD1), in contrast to other AAVs that interact primarily with PKD2. The AAVGo.1 cryo-EM structure of a complex with a PKD12 fragment of huAAVR at 2.4 Å resolution shows PKD1 bound with minimal change in virus structure, except for disordering of a neighboring surface loop. Only 4 of the 42 capsid protein sequence differences between AAVGo.1 and AAV5 occur at the PKD1 binding interface. These result in only minor conformational changes in AAVR, including a near rigid domain rotation with maximal displacement of the receptor by ~1 Å. A picture emerges of two classes of AAV with completely different modes of binding to the same AAVR receptor, but within each class atomic interactions are mostly conserved.IMPORTANCEAdeno-Associated Virus (AAV) is a small ssDNA satellite parvovirus. As a recombinant vector with a protein shell encapsidating a transgene, recombinant AAV (rAAV) is a leading delivery vehicle for gene therapy with two FDA-approved treatments and 150 clinical trials for 30 diseases. The human entry receptor huAAVR has five PKD domains. To date, all serotypes, except AAV5, have interacted primarily with the second PKD domain, PKD2. Goat is the AAV host most distant from human with cross-species cell infectivity. AAVGo.1 is similar in structure to AAV5, the two forming a class with a distinct mode of receptor-binding. Within the two classes, binding interactions are mostly conserved, giving an indication of the latitude available in modulating delivery vectors.
Phosphatidylserine within the Viral Membrane Enhances Chikungunya Virus Infectivity in a Cell-type Dependent Manner
Authors: Miazgowicz Kerri L. , Reyes Ballista Judith Mary , Acciani Marissa D. , Jimenez Ariana R. , Belloli Ryan S. , Duncan Avery M. , Havranek Katherine E. , Brindley Melinda A. 
Published: January 2022

AbstractChikungunya virus (CHIKV), an alphavirus of the Togaviridae family, is the causative agent of the human disease chikungunya fever (CHIKF), which is characterized by debilitating acute and chronic arthralgia. No licensed vaccines or antivirals exist for CHIKV. Preventing the attachment of viral particles to host cells is an attractive intervention strategy. Viral entry of enveloped viruses from diverse families including Filoviridae and Flaviviridae is mediated or enhanced by phosphatidylserine receptors (PSRs). PSRs facilitate the attachment of enveloped viruses to cells by binding to exposed phosphatidylserine (PS) in the viral lipid membrane - a process termed viral apoptotic mimicry. To investigate the role of viral apoptotic mimicry during CHIKV infection, we produced viral particles with discrete amounts of exposed PS on the virion envelope by exploiting the cellular distribution of phospholipids at the plasma membrane. We found that CHIKV particles containing high outer leaflet PS (produced in cells lacking flippase activity) were more infectious in Vero cells than particles containing low levels of outer leaflet PS (produced in cells lacking scramblase activity). However, the same viral particles were similarly infectious in NIH3T3 and HAP1 cells, suggesting PS levels can influence infectivity only in cells with high levels of PSRs. Interestingly, PS-dependent CHIKV entry was observed in mosquito Aag2 cells, but not C6/36 cells. These data demonstrate that CHIKV entry via viral apoptotic mimicry is cell-type dependent. Furthermore, viral apoptotic mimicry has a mechanistic basis to influence viral dynamics in vivo in both the human and mosquito host.ImportanceOutbreaks of Chikungunya virus (CHIKV) have occurred throughout Africa, Asia, and Europe. Climate change permits the expansion of Aedes mosquito vectors into more temperate regions, broadening the geographic range and increasing the frequency of future human outbreaks. The molecular basis underlying the broad host and cellular tropism of CHIKV remains unresolved. While several host molecules have been implicated in CHIKV viral attachment and entry, the role of lipid-mediated attachment (viral apoptotic mimicry) is unclear. We observed that higher levels of externalized phosphatidylserine (PS) in the viral lipid bilayer correlated with enhanced CHIKV infectivity in mammalian cells abundant with PS receptors and lacking alternative attachment factors. Interestingly, CHIKV infection in mosquito Aag2 cells was also affected by viral PS accessibility. This study further delineates the role of virus-cell attachment molecules in CHIKV infection. Viral apoptotic mimicry has potential to influence CHIKV dynamics in vivo in both the human and mosquito host.
EPSTEIN-BARR VIRUS LMP1 ENHANCES LEVELS OF MICROVESICLE-ASSOCIATED PD-L1
Authors: Harb Monica Abou , Sun Li , Meckes David G. 
Published: January 2022

AbstractExtracellular vesicles (EVs) circulate throughout the body and carry cargo that can be conferred to proximal or distant cells, making them major delivery vehicles for cellular communication. Epstein-Barr virus (EBV) infected cells release EVs that contain viral proteins such as the major viral oncogene, latent membrane protein 1 (LMP1). LMP1 has been shown to regulate the cellular gene expression of programmed cell death protein 1 ligand (PD-L1). PD-L1, a protein that suppresses the immune system by binding to PD-1, (a receptor found on cytotoxic T cells). PD-L1 has been recently found to be packaged into small EVs contributing to immune evasion of lung cancer cells. Recent studies establish that MVs are shed in very large amounts by tumor cells, and that elevated levels of MVs correlate to disease metastasis and cancers being more aggressive. Here, we demonstrate PD-L1 enrichment in MVs released from nasopharyngeal carcinoma cells and an important function of EBV LMP1 in regulating PD-L1 levels in MVs. These PD-L1+ MVs containing LMP1 likely contribute to the immunosuppressive microenvironment found in EBV-associated cancers.ImportanceAccumulating evidence over the past decade supports that viruses utilize EVs and associated pathways to incorporate viral products to evade eliciting an immune response, while concurrently enabling viral spread or persistence within the host. Considering that viral proteins confer very strong antigenic peptides that will be recognized by T cells, the regulation of the PD-1 pathway by the overexpression of MV-associated PD-L1 may be a strong immune evasion tactic utilized by viruses. The discovery that EBV LMP1 increases PD-L1 microvesicle secretion, identifies a new therapeutic target in immune blockade therapy. We expect that our findings will begin to clarify the mechanism of LMP1-mediated enhanced packaging of PD-L1 into MVs and may produce more specific targets to treat EBV-associated cancers. Consequently, identifying whether a disease is of viral origin through predictive MV biomarkers could further allow for more targeted therapies.
Information disclosing willingness in mobile internet contexts
Authors: Gao Bill Ming , Liu Matthew Tingchi , Chu Rongwei 
Published: January 2022

Purpose This paper aims to learn about consumers’ information disclosing patterns in the mobile internet context by investigating how demographic, geographic and psychological factors influence their information disclosing willingness (IDW).Design/methodology/approach Drawing on self-disclosure theory, the authors carried out simple linear regression analyses on a Chinese sample of 10,000 participants.Findings The results revealed that significant gender differences exist between males and females in their IDW in mobile internet context, and females have higher IDW than males do. And the authors also found that first-tier (third tier) citizens have the lowest (highest) IDW in their mobile internet usage.Originality/value This study offers three implications. First, this paper captures the insight of IDW within the mobile internet context, while previous studies mostly focus on the desktop internet context. Second, the results show that females have higher willingness to disclose than males do in the context of mobile internet, which is different from the findings of prior studies that females have higher privacy concerns and lower disclosing willingness in the context of desktop internet. Thirdly, this research introduces city tiers as a new approach to the study of IDW, which is one of the first studies exploring the geographical effect on information privacy.
The role of communication in restaurant crowdfunding success
Authors: Salvi Antonio , Raimo Nicola , Petruzzella Felice , Vitolla Filippo 
Published: January 2022

PurposeIn recent years, crowdfunding is assuming an increasingly central role in the development of business projects as an alternative financing tool to traditional sources. This study analyses the role of communication in the success of crowdfunding campaigns in the restaurant sector in the European context.Design/methodology/approachThis study conducts a regression analysis on a sample of 442 European restaurant crowdfunding projects launched on the Kickstarter platform in a time period spanning from 2014 to 2021. More specifically, this study uses a logistic regression model to test the impact of communication on the success of restaurant crowdfunding projects.FindingsEmpirical results suggest a strong impact of communication, declined in its different forms, on the success of restaurant crowdfunding campaigns. More specifically, they highlight a positive impact of the number of images, number of videos, readability and community orientation of the project description, number of comments and number of updates on the success of restaurant crowdfunding projects.Originality/valueTo the best of the authors’ knowledge, this study represents the first research that examines the effect of the communication on the success of restaurant crowdfunding projects conducted in the European context.
Consumer decision journey for online group buying: psychological and intentional procedure perspectives
Authors: Lin Shu-Chiung , Tseng Hsiao-Ting , Shirazi Farid 
Published: January 2022

PurposeThis study investigates the relationship between buyers’ psychological antecedent and repurchase intentions in online group buying. Considering and evaluating pre-purchase, the authors measure the buyers’ overall experience of online group buying through their perceived value (PV) and risk. This study examines the influence of PV and perceived risk (PR) on positive electronic word-of-mouth (eWoM) communication and repurchase intention in online group buying.Design/methodology/approachThe authors verify the research model using SPSS and the structural equation modeling-partial least square (SEM-PLS) to analyze 839 convincing respondents. This quantitative research provides insights into online group buying, including the food industry.FindingsThe results demonstrate that the buyers’ PV of online group buying enhanced positive eWoM communication and repurchase intention. The willingness of buyers to spread positive eWoM indicates their recognition and satisfaction with a product or brand. Thus, they have the behavioral intention to repurchase in online group buying. The study found that while positive eWoM communication significantly impacts repurchase intention, PR negatively affects PV. The research provides theoretical and practical implications at the end of the study.Research limitations/implicationsThis study has some limitations. First, the study only used PV and PR to measure consumers’ considerations and evaluations of the initial stage in the consumer decision journey. Future studies can include more dimensions that may further affect these considerations and assessments to verify the research model. Second, the study only used positive eWoM communication to measure the “enjoy-advocate-bond” of consumption and usage in the post-purchase stage.Originality/valueThe study proposes the sequential relationship in the psychological decision procedure of participating in online group buying and provides valuable managerial implications for managers operating online group buying on third-party platforms. The findings provide good knowledge for the food industry to stay connected with customers and develop their satisfaction.
Exploring the remote work challenges in the era of COVID-19 pandemic: review and application model
Authors: Arunprasad P. , Dey Chitra , Jebli Fedwa , Manimuthu Arunmozhi , El Hathat Zakaria 
Published: January 2022

PurposeRemote work (RW) literature is a megatrend in HRM literature, and the COVID-19 pandemic has highlighted the importance of RW as a concept and an organisational practice. Given the large number of papers being published on remote work, there is a need for a critical review of the extant literature using bibliometric analysis. This paper examines the literature on remote working to identify the factors crucial for managing a remote workforce. This study uses the complex adaptive systems theory as a foundation to build a framework that organisations can use to manage their remote workforce, focusing on three outcomes: employee engagement, collaboration and organisational agility.Design/methodology/approachBibliometric analysis was conducted on the research published in Scopus journal in the area of remote work, followed by critical literature analysis.FindingsThe bibliometric analysis identified five clusters that reflect five organisational factors which the management can align to achieve the desired outcomes of engagement, collaboration and agility: technology orientation, leadership, HRM practices, external processes and organisational culture. The present findings have important implications for managing the remote workforce.Originality/valueThe five factors were mapped to propose a conceptual model on engaging individual employees, fostering team collaboration and building organisational agility while working remotely. We also propose an application model for using technology to achieve the outcomes of engagement, collaboration and agility in the organisation. Practitioners could use this framework to focus on the factors that can create a conducive environment to improve work efficiency in a remote workforce.
Liquidity of China’s agricultural futures market: measurement and cross-market dependence
Authors: Xu Yuanyuan , Li Jian , Wang Linjie , Li Chongguang 
Published: January 2022

PurposeThis paper aims to present the first empirical liquidity measurement of China’s agricultural futures markets and study time-varying liquidity dependence across markets.Design/methodology/approachBased on both high- and low-frequency trading data of soybean and corn, this paper evaluates short-term liquidity adjustment in Chinese agricultural futures market measured by liquidity benchmark and long-term liquidity development measured by liquidity proxies.FindingsBy constructing comparisons, the authors identify the seminal paper of Fong, Holden and Trzcinka (2017) as the best low-frequency liquidity proxy in China’s agricultural futures market and capture similar historical patterns of the liquidity in soybean and corn markets. The authors further employ Copula-generalized autoregressive conditional heteroskedasticity models to investigate liquidity dependence between soybean and corn futures markets. Results show that cross-market liquidity dependence tends to be dynamic and asymmetric (in upper versus lower tails). The liquidity dependence becomes stronger when these markets experience negative shocks than positive shocks, indicating a concern on the contagion effect of liquidity risk under negative financial situations.Originality/valueThe findings of this study provide useful information on the dynamic evolution of liquidity pattern and cross-market dependence of fastest-growing agricultural futures in the largest emerging economy.
An improved Newton-Raphson based linear power flow method for DC grids with dispatchable DGs and ZIP loads
Authors: Li Hongwei , Wang Xiao , Lin Junmu , Wu Lei , Liu Tong 
Published: January 2022

Purpose
This study aims to provide a solution of the power flow calculation for the low-voltage ditrect current power grid. The direct current (DC) power grid is becoming a reliable and economic alternative to millions of residential loads. The power flow (PF) in the DC network has some similarities with the alternative current case, but there are important differences that deserve to be further concerned. Moreover, the dispatchable distributed generators (DGs) in DC network can realize the flexible voltage control based on droop-control or virtual impedance-based methods. Thus, DC PF problems are still required to further study, such as hosting all load types and different DGs.

Design/methodology/approach
The DC power analysis was explored in this paper, and an improved Newton–Raphson based linear PF method has been proposed. Considering that constant impedance (CR), constant current (CI) and constant power (CP) (ZIP) loads can get close to the practical load level, ZIP load has been merged into the linear PF method. Moreover, DGs are much common and can be easily connected to the DC grid, so V nodes and the dispatchable DG units with droop control have been further taken into account in the proposed method.

Findings
The performance and advantages of the proposed method are investigated based on the results of the various test systems. The two existing linear models were used to compare with the proposed linear method. The numerical results demonstrate enough accuracy, strong robustness and high computational efficiency of the proposed linear method even in the heavily-loaded conditions and with 10 times the line resistances.

Originality/value
The conductance corresponding to each constant resistance load and the equivalent conductance for the dispatchable unit can be directly merged into the self-conductance (diagonal component) of the conductance matrix. The constant current loads and the injection powers from dispatchable DG units can be treated as the current sources in the proposed method. All of those make the PF model much clear and simple. It is capable of offering enough accuracy level, and it is suitable for applications in DC networks that require a large number of repeated PF calculations to optimize the energy flows under different scenarios.