bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cadophora Species from Marine Glaciers in the Qinghai-Tibet Plateau
Authors: Zhang Bingqian , Li Xiaoguang , Li Guojie , Wang Qi-Ming , wang Manman 
Published: February 2022

Abstract
Large numbers of marine glaciers in the Qinghai-Tibet Plateau are especially sensitive to changes of climate and surface conditions and they have suffered fast accumulation and melting and retreated quickly in recent years. In 2017, we surveyed the cold-adapted fungi in these unique habitats and obtained 1208 fungal strains. Based on preliminary analysis of ITS sequences, 41 isolates belonging to the genus Cadophora were detected. As one of the most frequently encountered genera, the Cadophora isolates were studied in detail. Two phylogenetic trees were constructed: one was based on the partial large subunit nrDNA (LSU) to infer taxonomic placement of our isolates and the other was based on multi-locus sequences of LSU, ITS, TUB and TEF-1α to investigate more exact phylogenetic relationships of Cadophora and allied genera. Combined with morphological characteristics, nine Cadophora species were determined, including seven new to science. Among the new species, only C. inflata produced holoblastic conidia and all the others had phialidic conidiogenesis, and some isolates recognized as C. qinghai-tibetana and C. psychrophila had optimum growth temperature at 15°C. With more species involved, the currently circumscribed genus became obviously paraphyletic and all members clustered into two main clades: one clade mainly included most of the Cadophora species which had phialidic conidiogenesis and we referred to as ‘Cadophora s. str.’; the rest Cadophora species had multiform conidiogenesis and clustered in the second clade, with members of other genera in Ploettnerulaceae interspersed among the subclades. Our results showed a high diversity of Cadophora from marine glaciers in the Qinghai-Tibet Plateau and further intense sampling should be necessary for exploring more new species to reconstruct the phylogeny of this important fungal group.
Laser Remelting of AlSi10Mg(-Ni) Alloy Surfaces: Influence of Ni Content and Cooling Rate on the Microstructure
Authors: de Moura Danusa Araújo , de Gouveia Guilherme Lisboa , Figueira Gustavo , Garcia Amauri , Gargarella Piter , Spinelli José Eduardo 
Published: February 2022

Abstract
AlSi10Mg alloys are widely employed in a variety of industries, including aerospace, automotive, and microelectronics. This is because of its low density, acceptable mechanical properties, acceptable corrosion resistance, and inexpensive application cost. Advantageous fluidity, a short solidification period, and minimal volumetric contraction are beneficial characteristics under processing such alloys. Despite being used as commercial alloys, the mechanical properties of the AlSi10Mg alloys still need to be improved. In line with this, the current focus of Al-based alloys development is mostly on modifying commercially available alloys. Under such context, Ni was used as an alloying element in this study to generate the Al3Ni intermetallics, distinguished by its improved mechanical strength. Furthermore, the thermal stability of the Al3Ni may be a benefit, particularly for high-temperature applications. The present study aims to investigate the solidification under low and high cooling rates of four alloys: AlSi10Mg, AlSi10Mg-1Ni, AlSi10Mg-2Ni, and AlSi10Mg-3Ni (wt.%). Samples were obtained by directional solidification (DS) and laser surface remelting (LSR) processes. The cooling rates were calculated for the DS samples and with extrapolation for LSR samples as well as with the use of a model from the literature. After testing several laser conditions, the results also include an examination of microstructural and hardness changes in the treated and untreated zones. The produced gradient of microstructures is fully characterized as well as used to evaluate cooling rates inside the laser molten pools. For energy densities of 400 J/mm2 and 100 J/mm2, the mean dendritic spacings, λ, of the three Ni-containing alloys at the laser molten pool yelded estimated cooling rates of approximately 1.5 104 oC/s and 4.7 104 oC/s, respectively. A model explaining the reversion of λ across the molten pool will be outlined.
TRIM3 And TRIM16 As Tumor Suppressors In Breast Cancer Patients
Authors: Roshanazadeh Mohammad Reza , Adelipour Maryam , Sanaei Arash , Dariuni Hossein Azizi , Rashidi Mojtaba , Razavi Amirnader Emami 
Published: February 2022

Abstract
Background: Breast cancer is the leading cause of death among women in many countries. Clinicopathological parameters such as stage are important in cancer. Numerous factors serve as oncogenes or tumor suppressors in breast cancer. The large family of Tripartite-motif (TRIM) proteins with ~80 members has drawn attention for their role in cancer. TRIM3 and TRIM16 have shown suppressive activity in different cancers. This study aimed to evaluate the expression of TRIM3 and TRIM16 in cancerous and normal breast samples and investigate their association with different clinicopathological parameters.Methods and Results: 40 pairs of breast cancer and respective normal breast tissue samples, were selected. qRT-PCR with specific primer pairs were utilized to determine the gene expression of TRIM3 and TRIM16. With the reaction efficiency being >90%, the relative gene expression was calculated through the 2-∆∆Ct formula. The expression of TRIM3 and TRIM16 genes in tumor samples was significantly reduced to 0.45 and 0.29 fold, respectively, compared to the normal samples. TRIM3 and TRIM16 genes expression were both positively correlated with the invasion of breast cancer. TRIM3 gene expression was associated with tumors’ histological grade. However, no significant association was found between the expression of either of genes and tumor size, stage and necrosis.Conclusion: The expression of TRIM3 and TRIM16 genes are significantly reduced in breast cancer tissues. Besides, the expression of both TRIM3 and TRIM16 genes significantly plummet in lymphatic/vascular and perineural invasive samples. Hence, we suggest a potential tumor suppressor role for TRIM3 and TRIM16 in breast cancer.
Clinical Exome Sequencing for Inherited Retinal Disorders at a Tertiary Care Center
Authors: Ganapathi Mythily , Thomas-Wilson Amanda , Buchovecky Christie , Dharmadhikari Avinash , Barua Subit , Lee Winston , Ruan Merry ZC , Soucy Megan , Ragi Sara , Tanaka Joy , Clark Lorraine N. , Naini Ali B. , Liao Jun , Mansukhani Mahesh , Tsang Stephen , Jobanputra Vaidehi 
Published: February 2022

Abstract
Purpose: Inherited retinal disorders are clinically, and genetically heterogeneous diseases characterized by progressive deterioration of vision. This study aimed at assessing the diagnostic yield of exome sequencing (ES) for an unselected cohort of individuals with hereditary retinal disorders.Design: It is a retrospective study of 357 unrelated affected individuals, diagnosed with retinal disorders who underwent clinical ES.Methods: Variants from ES, were filtered, prioritized, and classified using the ACMG recommendations. Results: Clinical diagnosis of the individuals included -rod-cone dystrophy (59%), macular dystrophy (20%), cone-rod dystrophy (9%), cone dystrophy (4%) and other phenotypes (8%). Majority of the cases (74%) were singletons and 6% were trios. A confirmed molecular diagnosis was obtained in 23% of cases. In 6% of cases, two pathogenic variants were identified with phase unknown, bringing the potential molecular diagnostic rate to ~29%. Including the variants of uncertain significance (VUS), potentially significant findings were reported in 63% of cases. Among cases with a confirmed molecular diagnosis, variants in EYS, ABCA4, USH2A, KIZ, CERKL, DHDDS, PROM1, NR2E3, CNGB1, ABCC6, PRPH2, RHO, PRPF31, PRPF8, SNRNP200, RP1, CHM, RPGR were identified in more than one affected individual. Conclusions: Our results support the utility of clinical ES in the diagnosis of genetically heterogeneous retinal disorders.
Comparative Effect of Aqueous Extract of Psidium guajava Leaves and Arthemetar/Lumefantrine (coartem) on non-targeted parameters in Plasmodium berghei- Infected Mice
Authors: Kanu Shedrach , Ejeje Jerius , Elom Nwabueze , Eleazu Chinedu , Obeten Uket 
Published: February 2022

Abstract
Objective
To study the effect of aqueous extract of Psidium guajava and arthemeter/lumefantrine (coartem) on non-targeted parameters in Plasmodium berghei-infected mice.
Methods
Twenty five mice (out of thirty mice weighing between 30 and 35 g) were intraperitoneally infected with 0.2 ml of 1x105/ml parasitized erythrocytes of Plasmodium berghei (NK 65 strain) and randomly grouped into six (G2-G6) with five (5) mice each. G1 (normal control) received distilled water and animal feed. G2 (parasite control) received 1ml of distilled water daily. Groups (G3-G6) were treated with 28 mg/kg b.w of coartem, 200 mg/kg b.w, 400 mg/kg b.w and 600 mg/kg b.w of Psidium guajava extract respectively. Data were entered and analyzed using SPSS-21 statistical software. A p < 0.05 was taken as statistically significant.
Results
The Psidium guajava aqueous extract revealed presence of saponins, alkaloids, tannins, terpenoids and flavonoids. All the groups treated with Psidium guajava aqueous extract showed a dose-dependent significant (p < 0.05) increase in percentage clearance rate of the malaria parasite, PCV, HB, and RBC count, antioxidant status, antioxidant enzymes activities and a significant (p < 0.05) decrease in MDA level, ALP, AST and ALT activities as against the coartem treated and the parasite control groups.
Conclusion
Aqueous extract of Psidium guajava leaf could be useful in the treatment of diseases and symptoms caused by plasmodium parasites, especially berghei species with milder side effects than coartem.
Parameter Estimation of Uncertain Fractional-order Chaotic Power System Using an Improved State Transition Algorithm
Authors: Ai Chunyu , He Shan 
Published: February 2022

Abstract
Fractional-order chaotic power system parameter estimation is a multidimensional function optimization problem, and it is a prerequisite for controlling such systems. Aiming at the problem that the parameter estimation of a fractional-order chaotic power system is easily affected by external interference and estimation is difficult, a state transition algorithm based on the reverse learning strategy of lens imaging is proposed, taking a fractional six-order chaotic power system as the study model. First, the tent chaotic mapping to initialize the population, thus increasing population diversity. Second, we optimize parameters \(\gamma\) and \(\delta\) of the basic state transition algorithm to improve its global and single-dimensional search abilities. We use the lens imaging learning strategies to avoid the local optima. The proposed improved state transition algorithm shows high estimation accuracy and convergence speed, and is superior to the traditional state transition, particle swarm optimization, genetic and gray wolf algorithms. The simulation results show that the parameters of the fractional sixth-order chaotic power system are identified accurately. even in the presence of white noise, demonstrating the strong robustness and versatility of the proposed algorithm.
Genome-wide investigation of histone acetyltransferase gene family and their responses to biotic and abiotic stress in foxtail millet (Setaria italica [L.] P. Beauv)
Authors: Xing Guofang , Jin Minshan , Qu Ruifang , Zhang Jiewei , Han Yuanhuai , Han Yanqing , Wang Xingchun , Li Xukai , Ma Fangfang , Zhao Xiongwei 
Published: February 2022

Abstract
Background: Modification of histone acetylation is a ubiquitous and reversible process in eukaryotes and prokaryotes and plays crucial roles in the regulation of gene expression in plant development and stress responses. Histone acetylation is co-regulated by histone acetyltransferase (HAT) and histone deacetylase (HDAC). HAT activity plays an essential regulatory role in a variety of growth and development processes by modifying the chromatin structure through interactions with other histone modifications and transcription factors in eukaryotic cells, affecting the transcription of genes. Comprehensive analyses of HAT genes have been performed in Arabidopsis thaliana and Oryza sativa. However, little information is available on HAT genes in foxtail millet (Setaria italica [L.] P. Beauv).Results: In this study, 24 HAT genes (SiHATs) were identified and divided into four groups with conserved gene structures via motif compositions analysis. Phylogenetic analysis of the genes was performed to predict functional similarities between Arabidopsis thaliana, Oryza sativa, and foxtail millet; 19 and 2 orthologous gene pairs were individually identified. Moreover, all identified HAT gene pairs likely underwent purified selection based on their non-synonymous/synonymous nucleotide substitutions. Using published transcriptome data, we found that SiHAT genes were preferentially expressed in some tissues and organs. Stress responses were also examined, and data showed that SiHAT gene transcription was influenced by drought, salt, low nitrogen, and low phosphorus stress, and that the expression of four SiHATs was altered by Sclerospora graminicola infection. Conclusions: Results indicated that histone acetylation may play an important role in plant growth and development and stress adaptations. These findings suggest that SiHATs play specific roles in the response to abiotic stress and viral infection. This study lays a foundation for further analysis of the biological functions of SiHATs in foxtail millet.
Effects Of Kinetic Changes On Neuromuscular Activity Of Adductor Magnus During Bridging Exercises
Authors: HE Shutang , Li Yashu , Tan Jiaqi , Zhang Shanshan , Wang Chuhuai 
Published: February 2022

Abstract
Background: Most therapeutic exercises can activate the adductor magnus (AM) at a high to a very high level. It is necessary to find an exercise that can facilitate AM at low to moderate levels in the early stage of sports rehabilitation. This study is aimed to investigate changes in AM activation with various knee kinetic positions during bilateral bridging exercise (BBE) and unilateral bridging exercise (UBE).Method: 20 adults (7 males and 13 females, aged 20-30) volunteered for this study. Surface electromyography (EMG) signals were recorded from AM at four knee flexion angles: 45°, 60°, 90°, and 120° when performing UBE and BBE. The obtained raw data were normalized by the corresponding muscle's maximal voluntary isometric contraction (MVIC). Results: AM recruitment levels were 15.0±8.5% MVIC in BBE and 35.1±21.6% MVIC in UBE. There was no significant difference in the EMG amplitude of AM activity among various knee flexion angles during both UBE and BBE. Conclusions: BBE and UBE can be utilized as therapeutic exercises to facilitate AM from low to moderate intensity at partly weight-bearing positions.
The Effect of Virtual Cognitive-Behavioral Sexual Counseling on Sexual Function and Intimacy in Pregnant Women: a Randomized Controlled Clinical Trial
Authors: Fathalian Manizheh , Lotfi Razieh , Faramarzi Mahbobeh , Qorbani Mostafa 
Published: February 2022

Abstract
Background & Aim: Pregnancy is associated with numerous physical and psychological changes and affects the sexual function of couples. Since the root of many marital problems lies in the quality of the relationship and sexual function, the present study investigates the effect of virtual cognitive-behavioral sexual counseling on pregnant women's sexual function and intimacy.Methods & Materials: This study is a randomized clinical trial, and 80 pregnant women aged 18 to 35 years and in 16-24 weeks of pregnancy were assigned to two intervention and control groups based on randomized blocks. The control group received routine prenatal care, but the intervention group, in addition to routine prenatal care, underwent virtual counseling with a cognitive-behavioral approach during eight sessions of 90 minutes. Data were collected using the Female Sexual Function Index (FSFI) and sexual intimacy questionnaire. SPSS software was used for statistical analysis.Results: The comparison of mean scores of sexual function and intimacy in the two groups before the intervention was not statistically significant with each other. However, after the intervention, the mean scores of sexual function and intimacy in the intervention group were significantly increased compared to the control group (p<0.001). The effect size of the intervention was 0.52 for sexual function and 0.272 for sexual intimacy.Conclusion: Virtual cognitive-behavioral counseling can be used as an effective treatment to promote sexual function and intimacy of women during pregnancy.Clinical trial registry: IRCT20161230031662N10Registry date: 21/06/2021
Microorganisms Isolated From the Bile of the Patients who have Undergone Cholecystectomy and Their Antibiotic Resistance Pattern: Multicenter Prospective Study
Authors: Ozturk-Engin Derya , Agalar Canan , Yasemin Cag , Kesmez-Can Fatma , Balkan İlker İnanç , Karabay Oguz , Senbayrak Seniha , Cetinkaya Busra Meral , Aydın Mehmet Timucin , Tomas Kadir , Disci Esra , Surmelioglu Ali , Alimoglu Orhan , Ekinci Ozgur , Akın Emrah , Koroglu Mehmet , Velidedeoglu Mehmet , Ankaralı Handan , Kocoğlu Esra , Javadov Mirkhaliq , Papila-Kundaktepe Berrin , Oguzoglu Naz , Ozmen Erkan , Donmez Ramazan , Mega Ertunc , Aksaray Sebahat , Agalar Fatih 
Published: February 2022

Abstract
Background Gallbladder and biliary tract infections are diseases with high mortality rates if they are not treated properly. Microbiological evaluation of perioperatively collected samples both ensures proper treatment of patients and guides empirical treatment due to the determination of microorganism susceptibility. Aim This study aimed to isolate the microorganisms in bile cultures from patients who underwent cholecystectomy and to determine sensitivity results of these microorganisms. Methods This study was a multi-center and prospective design, included 360 patients, and was performed between 2019 and 2020. Culture results of bile taken during cholecystectomy were evaluated. Results Bacterial growth was found in the bile cultures of 84 out of 360 (23.3%) patients. Patients were divided into two groups according to whether they had risk factors for resistant microorganisms or not. While Escherichia coli (n = 11, 13%), Enterococcus spp. (n = 8, 9.5%), and Enterobacter spp. (n=4, 4.7%) were detected most frequently in patients without risk. Staphylococcus spp. (n = 17, 20.2%), Enterococcus spp., (n = 16, 19%), and E. coli (n = 8, 9.5%) were the most frequently found microorganism in risky patients. Bile culture positivity was higher in males (p = 0.001), > 60 years (p = 0.010), history of biliary disease (p = 0.002), underwent biliary surgery before cholecystectomy (p = 0.002), having biliary instrumentation (p = 0.014), operation performed concurrently with a cholecystectomy (p = 0.001), and presence of complications (p = 0.001). Conclusions Our study shows that when starting empirical antibiotic treatment for bile ducts, whether patients are at risk for the development of resistant bacterial infection should be evaluated after which antibiotic selection should be made accordingly.
Synthesis, antimicrobial, PASS, SAR, molecular docking, molecular dynamics and pharmacokinetic studies of designed benzylidene derivatives
Authors: Kawsar Sarkar M. A. , Hosen Mohammad Imran , Celik Ismail , Affi Sopi Thomas , Erol Meryem , Islam Sajia , Dey Sujan , Hosen Anowar 
Published: February 2022

Abstract
The present study is focused on the synthesis of methyl-4,6-O-benzylidene-α-D-glucopyranoside (MBG) derivatives and on the characterization of their biological properties. Their structures were revealed by spectroscopic characterization and antimicrobial activities were evaluated in vitro against human and plant microorganisms along with the prediction of substance activity spectra. Minimum inhibition concentration (0.675±0.01 to 1.35±0.01 mg/ml) and minimum bactericidal concentration (1.35±0.01 to 5.40±0.04 mg/ml) tests were conducted for two compounds (4 and 7) based on their activity. X-ray powder diffraction was employed for quantitatively identifying crystalline compounds. Molecular docking studies focused on fungal proteins and bacterial proteins where derivatives 3 and 6 exhibited very strong binding and inhibitory affinities. Also, the molecular dynamics study carried out revealed that the complexes formed by these derivatives with the proteins L,D-transpeptidase Ykud and endoglucanase from Aspergillus niger can remain stable both over time and in physiological conditions. Structure-activity relationships study including in vitro and silico results revealed that the acyl chains [lauroyl (C12), cinnamoyl (C6H5CH=CHCO-)] in combination with sugar were found the most potential activates against human and fungal pathogens. Our synthetic, antimicrobial, and pharmacokinetic studies revealed that MBG derivatives have promising antimicrobial activity, which could develop a therapeutic target for bacteria and fungi.
Efficient binary and QAM optical modulation in ultra-compact MZI structures utilizing indium-tin-oxide
Authors: Mohammadi-Pouyan Sohrab , Miri Mehdi , Sheikhi Mohammad Hossein 
Published: February 2022

Abstract
A design for a CMOS-compatible active waveguide is proposed in which the epsilon-near-zero (ENZ) property of the Indium-tin-oxide (ITO) is used to induce large variations in the real and imaginary parts of the waveguide effective index. The proposed waveguide comprises a TiN/HfO2/ITO metal-oxide-semiconductor (MOS) structure where the speed and power consumption are significantly reduced by the application of the TiN and realization of double accumulation layers in the ITO. Simulations show the insertion loss (IL) of 0.38dB/µm, extinction ratio (ER) of 11dB/µm, the energy consumption of 11.87fJ/bit and electrical bandwidth of 280GHz when the designed waveguide is used as an electro-absorption modulator. The waveguide is then used in an MZI structure to design binary and quadrature-amplitude-modulator (QAM) modulators. For binary modulator, the IL, ER, and VπLπ figures of merit are found to be 1.24dB, 54dB, and 6.4V.µm, respectively, which show substantial improvement over previous ITO-based designs. In the QAM design, the symmetry in the real and imaginary parts of the waveguide effective index is employed to obviate the need for additional phase shift elements. This considerably reduces the overall length of the proposed QAM modulator and improves efficiency. Simulations show the energy consumption and bit rate, of 2fJ/bit and 560Gbps, respectively in a 4-QAM modulator with the overall length of 6.2µm. The symmetry properties of the proposed waveguide can be further exploited to realize quadrature-phase-shift-keying (QPSK) modulators which here is used in combination with the 4-QAM to propose a design for the more advanced modulation scheme of 16-QAM. The design of ITO-based QAM modulators is here reported for the first time and the abovementioned performance parameters show the unique properties of these modulators in terms of footprint, energy consumption and modulation-speed.