bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial protein import stress augments α-synuclein aggregation and neurodegeneration independent of bioenergetics
Authors: Coyne Liam P. , Rana Arnav , Wang Xiaowen , Bhagwagar Sanaea , Umino Yumiko , Solessio Eduardo C. , Middleton Frank , Chen Xin Jie 
Published: September 2022

ABSTRACTSeveral genetic and environmental risk factors for Parkinson’s disease have been identified that converge on mitochondria as central elements in the disease process. However, the mechanisms by which mitochondrial dysfunction contributes to neurodegeneration remain incompletely understood. Non-bioenergetic pathways of the mitochondria are increasingly appreciated, but confounding bioenergetic defects are a major barrier to experimental validation. Here, we describe a novel bioenergetics-independent mechanism by which mild mitochondrial protein import stress augments neurodegeneration. We induced this mitochondrial protein import stress in an established mouse model of Parkinson’s disease expressing the A53T mutated form of α-synuclein (SNCA). Mice with import stress in addition to the A53T mutation demonstrated increased size of α-synuclein aggregates, co-aggregation of mitochondrial preproteins with α-synuclein, and worsened neurodegeneration. Importantly, we found no evidence of bioenergetic defects in any of the mutant mice, even with the added import stress. These data suggest that mitochondrial protein import stress contributes to neurodegeneration through cytosolic proteostatic stress and co-aggregation of mitochondrial and neuropathogenic proteins independent of bioenergetics. Given that protein import efficiency is affected by many types of mitochondrial stress, our findings add a new layer to understanding why the pathogenic mitochondrial dysfunction and cytosolic protein misfolding pathways converge in neurodegenerative diseases such as Parkinson’s disease.
Comparative epidemic expansion of SARS-CoV-2 variants Delta and Omicron in Amazonas, a Brazilian setting with high levels of hybrid immunity
Authors: Arantes Ighor , Bello Gonzalo , Nascimento Valdinete , Souza Victor , da Silva Arlesson , Silva Dejanane , Nascimento Fernanda , Mejía Matilde , Brandão Maria Júlia , Gonçalves Luciana , Silva George , da Costa Cristiano Fernandes , Abdalla Ligia , Santos João Hugo , Ramos Tatyana Costa Amorim , Piantham Chayada , Ito Kimihito , Siqueira Marilda Mendonça , Resende Paola Cristina , Wallau Gabriel Luz , Delatorre Edson , Gräf Tiago , Naveca Felipe 
Published: September 2022

ABSTRACTThe SARS-CoV-2 variants of concern (VOCs) Delta and Omicron spread globally during mid and late 2021, respectively, with variable impact according to the immune population landscape. In this study, we compare the dissemination dynamics of these VOCs in the Amazonas state, one of Brazil’s most heavily affected regions. We sequenced the virus genome from 4,128 patients collected in Amazonas between July 1st, 2021 and January 31st, 2022 and investigated the lineage replacement dynamics using a phylodynamic approach. The VOCs Delta and Omicron displayed similar patterns of phylogeographic spread but significantly different epidemic dynamics. The Delta and Omicron epidemics were fueled by multiple introduction events, followed by the successful establishment of a few local transmission lineages of considerable size that mainly arose in the Capital, Manaus. The VOC Omicron spread and became dominant much faster than the VOC Delta. We estimate that under the same epidemiological conditions, the average Re of Omicron was ∼3.3 times higher than that of Delta and the average Re of the Delta was ∼1.3 times higher than that of Gamma. Furthermore, the gradual replacement of Gamma by Delta occurred without an upsurge of COVID-19 cases, while the rise of Omicron fueled a sharp increase in SARS-CoV-2 infection. The Omicron wave displayed a shorter duration and a clear decoupling between the number of SARS-CoV-2 cases and deaths compared with previous (B.1.* and Gamma) waves in the Amazonas state. These findings suggest that the high level of hybrid immunity (infection plus vaccination) acquired by the Amazonian population by mid-2021 was able to limit the spread of the VOC Delta and was also probably crucial to curb the number of severe cases, although not the number of VOC Omicron new infections.
Under the shadow: Old-biased genes are subject to weak purifying selection at both the tissue and cell type-specific levels
Authors: Yıldız Melih , İzgi Hamit , Rahimova Firuza , Altıntaş Umut Berkay , Turan Zeliha Gözde , Somel Mehmet 
Published: September 2022

AbstractThe mutation accumulation theory predicts that aging is caused by accumulation of late-acting deleterious variants in the germ-line, due to weak purifying selection at old age. In accordance with this model, we and others have shown that sequence conservation among old-biased genes (with higher expression in old versus young adults) is weaker than among young-biased genes across a number of mammalian and insect species. However, questions remained regarding the source and generality of this observation. It was especially unclear whether the observed patterns were driven by tissue and cell type composition shifts or by cell-autonomous expression changes during aging. How wide this trend would extend to non-mammalian metazoan aging was also uncertain. Here we analyzed bulk tissue as well as cell type-specific RNA sequencing data from diverse animal taxa across six different datasets from five species. We show that the previously reported age-related decrease in transcriptome conservation (ADICT) is commonly found in aging tissues of non-mammalian species, including non-mammalian vertebrates (chicken brain, killifish liver and skin) and invertebrates (fruit fly brain). Analyzing cell type-specific transcriptomes of adult mice, we further detect the same ADICT trend at the single cell type level. Old-biased genes are less conserved across the majority of cell types analyzed in the lung, brain, liver, muscle, kidney, and skin, and these include both tissue-specific cell types, and also ubiquitous immune cell types. Overall, our results support the notion that aging in metazoan tissues may be at least partly shaped by the mutation accumulation process.
Autoinsertion (LAiR): rapid functional reconstitution of integral membrane proteins into lipid bilayers
Authors: Godoy-Hernandez Albert , Asseri Amer H. , Purugganan Aiden J. , Jiko Chimari , de Ram Carol , Lill Holger , Pabst Martin , Mitsuoka Kaoru , Gerle Christoph , Bald Dirk , McMillan Duncan G. G. 
Published: September 2022

AbstractFunctional investigation of purified integral membrane proteins (IMPs) is hampered by the need to insert these hydrophobic proteins from the detergent-solubilized state into liposomal membranes. Here we report reintegration of IMPs into a lipid environment within minutes, an order of magnitude faster than currently used standard techniques. The new approach yielded optimal results for IMPs solubilized in the detergent lauryl-maltose neopentyl glycol (LMNG) and is therefore termed LMNG Auto-insertion Reintegration (LAiR). LAiR displays superior performance to standard methods in terms of protein activity, long-term stability and proton tightness of proteoliposomes. LAiR reconstituted vectorial control of membrane-bound activity by the transmembrane ion motive force, a property particularly important in mitochondrial function, which was undetectable by standard reintegration methods. LAiR also preserved fragile IMP properties that are prone to disruption upon reintegration, including long-term multi-subunit integrity, inhibitor susceptibility, and higher-order oligomeric states. LAiR proved suitable for reintegration into liposomes as well as into surface-tethered membrane bilayers, and was compatible with IMPs and lipids from prokaryotic and eukaryotic sources. We anticipate a broad scope for LAiR as a powerful tool in fundamental research, pharmaceutical applications, and biotechnology.
Study on bioactivity of SLMed variable gradient TC4 biomedical porous scaffolds with micro-arc oxidation treatment
Authors: Lv Yuting , Guo Jiawei , Huang Weimin , Liu Yaojie , Liu Wentao , Wei Guijiang 
Published: September 2022

Purpose
The purpose of this paper is to improve the bioactivity of variable gradient TC4 porous scaffolds prepared by selective laser melting (SLM) through the micro-arc oxidation (MAO) technique.

Design/methodology/approach
Variable gradient TC4 porous scaffolds were prepared by SLM, then treated with MAO at different oxidation voltages. The microstructure, thickness and composition of MAO coatings were characterized by scanning electron microscope (SEM), energy-dispersive spectroscopy (EDS) and X-ray diffraction. The bioactivity of the MAO coatings was tested by simulated body fluid (SBF) immersion test.

Findings
SEM and EDS results show that with the increase of oxidation voltage, the content of Ca and P elements and the thickness of the MAO coatings increases. The thickness of the coating inside the scaffold is smaller than that of the outside regions. SBF immersion experiments showed that MAO-treated TC4 porous scaffolds had highest bioactivity at 440 V.

Originality/value
The variable gradient porous scaffolds were treated with MAO in the electrolyte containing Ca and P elements for the first time. The effect of oxidation voltages on the different region of porous scaffolds was studied in detail.
Feminist futures in gender-in-leadership research: self-reflexive approximations to intersectional situatedness
Authors: Rodriguez Jenny K. , Guenther Elisabeth Anna , Faiz Rafia 
Published: September 2022

Purpose
This paper introduces intersectional situatedness to develop inclusive analyses of leadership. Intersectional situatedness recognises the contextual and situated nature of experiences and their interaction with socially constructed categories of difference.

Design/methodology/approach
The paper draws on memory work by three feminist academics who situate their understandings and experiences of leadership as part of socio-historical contexts.

Findings
Understandings and experiences of leadership are multifaceted and benefit from being examined in their intersectional situatedness. This way, the simultaneity of visible and invisible disadvantage and privilege, which accumulate, shift and get reconfigured across the life course and are based on particular intersectional identity invocations, can be integrated into narratives about leadership.

Research limitations/implications
Interrogating gender-in-leadership adopting an intersectional situatedness helps to advance the field by embedding the recognition, problematisation and theorisation of situated difference as critical to understand leadership, its meaning and its practice in management and organisations.

Practical implications
In embedding intersectional situatedness in the analysis of leadership, more inclusive understandings of leadership are qualified that recognise differences positively and support changing the narratives around the meaning of “leader” and “good leadership”.

Social implications
Intersectional situatedness helps to identify tangible ways to see how inequalities impact women’s career progression to leadership and enable more nuanced conversations about privilege and disadvantage to advance feminist social justice agendas.

Originality/value
The paper reveals the narrow and restricted understandings of leadership and how this influences who is regarded as a legitimate leader. In addition, it adopts a methodology that is not commonly used in gender-in-leadership research.
Ghost production: applying the Servuction model to establish a typology and propose a research agenda for on-demand restaurant food delivery
Authors: Ashton Mark , Tuomi Aarni , Backman Peter 
Published: September 2022

PurposeThe rapid growth in volume and value of on-demand restaurant food delivery, accelerated by the COVID-19 pandemic, is causing a paradigm shift in the food service sector. However, there is a lack of hospitality management research into this emerging phenomenon. To address this gap, this paper defines and develops a novel conceptual model and typology and proposes a research agenda for ghost production in the context of food service.Design/methodology/approachThis paper uses the Servuction model to explore, define and model the radical separation between food service production sites, points of sale and consumer interaction from the perspective of on-demand restaurant food delivery. A novel typology is developed and illustrated with eight industry examples from the UK and an accompanying cost benefit analysis. Future research priorities are identified.FindingsIn the hospitality literature, little attention has been paid to changes on-demand restaurant food delivery brings to production and business models of food service organisations, resulting in significant gaps between food service practice and theory. The knock-on effects to stakeholders include increased convenience for customers, uncertain employment status of riders and, for restaurants, striking a balance between capturing new markets and losing control of the customer. Additionally, for aggregators, there is a lack of profitability in existing models, despite holding the balance of power (and data).Originality/valueThe concept of “ghost production” and its associated typology is novel and offers a contribution to hospitality management literature by defining the term, scope and scale of this new phenomenon. Practical implications are proposed.
Online firestorms: an act of civic engagement or a narcissistic boost? The role of brand misconduct appraisals
Authors: Delgado-Ballester Elena , López-López Inés , Bernal Alicia 
Published: September 2022

PurposeThis study aims to examine personal factors that drive the initiation of an online firestorm, an online collaborative attack against a brand that usually occurs when a brand fails or engages in misconduct.Design/methodology/approachA structured questionnaire is used for data collection. Via an online panel, 303 questionnaires are completed. Hypotheses are tested using structural equation modelling.FindingsCivic engagement and, to a lesser extent, narcissism explain intentions to initiate online firestorms. Individuals’ inherent concern for others and for ethical issues prompts them to punish a brand publicly, with the expectation that other individuals will follow and that the brand will feel compelled to react. Misconduct-related appraisal factors, such as severity, proximity, moral inequity and outrage, can amplify the effect of civic engagement.Research limitations/implicationsFuture research should investigate whether the findings hold for different types of misconduct, cultures and other forms of narcissism.Practical implicationsBy providing managers with a better understanding of the motivations for launching an attack, this study offers guidance on managing a brand when an online firestorm occurs.Originality/valueWhereas previous research has focused on external drivers of online firestorms, this study builds on the prosocial behaviour literature to analyse the individual characteristics prompting the initiation of an online firestorm.
Extended belief rule-based method for the assessment of matching between rescue tasks and volunteers
Authors: Chen Li , Chen Sheng-Qun , Yang Long-Hao 
Published: September 2022

PurposeThis paper aims to solve the major assessment problem in matching the satisfaction of psychological gratification and mission accomplishment pertaining to volunteers with the disaster rescue and recovery tasks.Design/methodology/approachAn extended belief rule-based (EBRB) method is applied with the method's input and output parameters classified based on expert knowledge and data from literature. These parameters include volunteer self-satisfaction, experience, peer-recognition, and cooperation. First, the model parameters are set; then, the parameters are optimized through data envelopment analysis (DEA) and differential evolution (DE) algorithm. Finally, a numerical mountain rescue example and comparative analysis between with-DEA and without-DEA are presented to demonstrate the efficiency of the proposed method. The proposed model is suitable for a two-way matching evaluation between rescue tasks and volunteers.FindingsDisasters are unexpected events in which emergency rescue is crucial to human survival. When a disaster occurs, volunteers provide crucial assistance to official rescue teams. This paper finds that decision-makers have a better understanding of two-sided match objects through bilateral feedback over time. With the changing of the matching preference information between rescue tasks and volunteers, the satisfaction of volunteer's psychological gratification and mission accomplishment are also constantly changing. Therefore, considering matching preference information and satisfaction at two-sided match objects simultaneously is necessary to get reasonable target values of matching results for rescue tasks and volunteers.Originality/valueBased on the authors' novel EBRB method, a matching assessment model is constructed, with two-sided matching of volunteers to rescue tasks. This method will provide matching suggestions in the field of emergency dispatch and contribute to the assessment of emergency plans around the world.
Women social entrepreneurs in a Muslim society: how to manage patriarchy and spouses
Authors: Ummiroh Isnaini Ruhul , Schwab Andreas , Dhewanto Wawan 
Published: September 2022

Purpose
This study aims to investigate how women social entrepreneurs in Indonesia use various behaviors to address challenges to their leadership authority created by socioreligious patriarchal norms in this Muslim society.

Design/methodology/approach
An exploratory study of six Muslim women social entrepreneurs was conducted using multiround, semistructured interviews in a contrast sample of three women who work with their husbands and three women who work without their husband’s involvement.

Findings
The study identifies a variety of leadership behaviors that women entrepreneurs use to mitigate the constraining impact of strong patriarchal religious gender norms. Observations revealed surprisingly effective micro adjustments often based on relationship-specific private negotiations between the entrepreneurs and their husbands.

Research limitations/implications
Future research focused on the husbands’ perspectives and behaviors, as well as extensions to other patriarchal religions and societies, are encouraged.

Practical implications
Recognition of the crucial role of spousal relationships suggests the need for more holistic approaches to support women social entrepreneurship, e.g. by integrating husbands into related outreach programs.

Social implications
Religious gender stereotypes such as the stronger altruistic orientation of women can help counteract, to a degree, Muslim patriarchal norms when women lead social enterprises. Leadership of social enterprises by women promises to promote more gender equality over time, even if associated private and relationship-specific accommodations are not intended to challenge religious gender norms.

Originality/value
This study contributes to emerging research on the crucial role of spousal relationships for women’s entrepreneurship and the impact of private micro arrangements between spouses to mitigate the constraining impact of Muslim gender norms. Muslim women entrepreneurs approved of the religious gender norms that constrained them, in contrast to the more “feminist” perspectives common in women entrepreneurs in more secular and Christianity-dominated western societies.
Market‐wide shocks and the predictive power for the real economy in the Korean stock market
Authors: Kim Jinyong , Kim Yongsik 
Published: September 2022

AbstractWe examine the predictive power of the stock market return for real economic growth corresponding to market‐wide shocks in Korea. The periods of large market‐wide shocks, such as the COVID‐19 pandemic, are characterized by the stock return synchronicity and volatility that can measure price informativeness. We find that the predictive power of the stock market return disappears when stock returns show high synchronicity and volatility, and the deteriorated predictive power is associated with an increase in individual investors' net buying shares. Our finding suggests that, even if the stock market shows outstanding performance following market‐wide shocks, this signal should be interpreted with caution because the stock market return may become less informative about fundamentals and lose the predictive power for the real economy.
Collaborating toward equity in Pennsylvania: The Age‐Friendly Care, PA project
Authors: Berish Diane , Husser Erica , Knecht‐Fredo Jenny , Sabol Jacqueline , Garrow George , Hupcey Judith , Fick Donna 
Published: September 2022

AbstractObjectiveTo collaboratively implement the age‐friendly health systems framework, known as the 4Ms: What Matters, Medication, Mentation, and Mobility, at The Primary Health Network (PHN), a federally qualified health center.Data SourcesData were collected from PHN electronic medical records (EMRs) for individuals over age 65 from December 30, 2019 to December 24, 2021 and from Project ECHO© attendance and evaluation surveys.Study DesignThe telementoring educational program, Project ECHO©, was used to engage PHN health care professionals working in rural areas of Pennsylvania to incorporate the 4Ms into their practice starting with the annual wellness visit (AWV). Project ECHO© was launched at three primary care sites. After 18 months, it was then disseminated to an additional 18 sites creating pilot and comparison groups. Outcomes included codesigned patient process metrics using EMR data and project ECHO© participant data.Data Collection MethodsEMR data were generated by system reports created by PHN's quality assurance program manager. Project ECHO© data were collected and managed using REDCap electronic data capture tools. Outcomes were aggregated, analyzed for trends over time, and compared between groups.Principal FindingsAll nine process outcomes increased from baseline to follow‐up at the three initial sites, ranging from 4% to 43% g. At year two, the three initial sites had higher rates on AWVs (pilot 24%, comparison 12%; p < 0.0001), Advance Care Planning (New on file, pilot 8%, comparison 2%; Discussed with patient, pilot 18%, comparison 13%; Patient declined, pilot 0%, comparison 0%; p = 0.0001), Dementia Screening (pilot 24%, comparison 12%; p < 0.0001), Fall Risk Management (pilot 43%, comparison 10%; p < 0.0001), and Mobility Goal (pilot 19%, comparison 9%; p < 0.0001); and lower rates on High‐Risk Medication Elimination (pilot 54%, comparison, 63%, p < 0.02).ConclusionsAccess to high‐quality geriatric care for rural older adults can be improved by increasing health care professionals' knowledge of the 4Ms, beginning with its incorporation into the AWV.