bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term drought aggravates instability of alpine grassland productivity to extreme climatic event
Authors: He Yun L. , Wang Jin S. , Tian Da S. , Quan Quan , Jiang Lin , Ma Fang F. , Yang Lu , Zhang Fang Y. , Zhou Qing P. , Niu Shu L. 
Published: June 2022

AbstractThe frequency and severity of extreme weather events are increasing and expected to increase more in the future, together with global change. However, how extreme events and global change factors interactively influence community structures and ecosystem processes is largely unknown. Here, we investigated the responses of the temporal stability and resilience of aboveground net primary productivity (ANPP) of an alpine meadow to an extreme flooding event under different treatments of experimental drought and clipping. We found that ecosystems that were exposed to drought treatments for 3 years significantly decreased the temporal stability of community productivity but increased resilience to flooding, whereas their resistance to or recovery from flooding did not change. Neither clipping nor its interaction with drought altered the responses of these community stability metrics to flooding. Drought treatments significantly decreased plant species richness and asynchrony and dominant species stability, leading to a decrease in temporal stability and an increase in resilience in response to the extreme flooding event. The study also revealed that the change in species asynchrony was the dominant impact pathway determining the responses of resilience and temporal stability to flooding. Our results highlight that alpine grassland that experiences a multiyear drought may aggravate the instability of community productivity to extreme climatic events by reducing species asynchrony.
Concept, analysis, and test of a self‐centering viscous damper with displacement capacity enhancement
Authors: Li Jinping , Wu Bin , Wang Zhen , Yang Haowen , Xu Guoshan 
Published: June 2022

AbstractResilience of civil engineering structures is getting more and more attention from experts as well as public. Residual deformation has been used as an important index to measure the resilience of structures subjected to earthquakes. A self‐centering viscous damper (SCVD) is proposed with prestressed steel strand as recentering element and viscous oil to dissipate energy. A hydraulic mechanism for displacement capacity enhancement is designed to resolve the problem of insufficient deformation capacity of the recentering steel strands. The working principle, numerical modeling, specimen design, and cyclic tests of the SCVD are described in detail. A real‐time hybrid test of a structure incorporating the SCVD validates the excellent effect of the SCVD on the seismic residual deformation of the structure.
Spatial and temporal dynamics of gully erosion in anthropogenically modified forest and forest‐steppe landscapes of the European part of Russia
Authors: Yermolaev Oleg , Medvedeva Regina , Poesen Jean 
Published: June 2022

AbstractGully erosion is a major soil degradation process on arable land. Therefore, it is important to better understand the spatial and temporal patterns of this linear erosion process, considering the impacts of land use and climate change. The main objective of this study was to analyse the spatial and temporal patterns of permanent gully development in a study area of 123 300 km2 located in the eastern periphery of the European boreal zone (southern taiga, mixed and deciduous forests and forest‐steppe). In this study, visual interpretation of remote sensing data is used to detect changes in permanent gully channel length and density for each river basin between 1950/1970 and 2017/2021. Currently, 22 688 permanent gullies have been identified in the study area, their average length being 64 m, with the total gully network length reaching 1450.3 km. The average density of the gully network for 2017/2021 is 16 m km−2; the maximum value reaching 471 m km−2. During the period under study (c. 50 years), there was a widespread reduction in gully density by 184 m km−2 on average (i.e. from 100–500 m km−2 in 1950/1970 to <10 m km−2 in 2017/2021). Linear and areal channel changes were determined for 304 active gullies over the 2009/2016 period. The average linear gully growth rate during this period is 0.6 m year−1, whereas the areal growth rate is 28 m2 year−1. There is a clear reduction of gully erosion in the study area. Analysis of the gully network density in relation to land use changes (i.e. cultivated, forest and pasture area) did not reveal any significant relationship. It is concluded that the reduction of gully density in the study area is caused by the natural evolution of these linear erosion forms and their transformation into balkas (i.e. small, dry, flat‐bottomed valleys).
Evaluation of sensory and safety quality characteristics of “high mountain tea”
Authors: Wang Cong‐ming , Du Xiao , Nie Cong‐ning , Zhang Xiang , Tan Xiao‐qin , Li Qian 
Published: June 2022

AbstractHigh mountain tea (HT) is widely acknowledged as an essential resource of high‐quality tea due to its adaptation to superior ecological environments. In this study, the sensory (aroma and taste) and safety (heavy metals and pesticide residues) characteristics of HT were characterized through sensory evaluation, gas chromatography–mass spectrometry (GC‐MS), liquid chromatography–mass spectrometry (LC‐MS), flavor activity value, and risk factor analysis. The results elucidated that the aroma sensory characteristics of HT were tender and green, accompanied by sweet and slight chestnut. A total of 8 aroma compounds were identified as the primary substances contributing to the unique aroma characteristics; the difference in the ratio of "green substances" and "chestnut substances" might be the reason for different aroma characteristics in HT and LT (low mountain tea). The taste sensory characteristics of HT were high in freshness and sweetness but low in bitterness and astringency. The high content of soluble sugar (SS), nonester catechins, sweet free amino acids, and low content of caffeine and tea polyphenols were the primary reasons for its taste characteristics. Low temperature stress might be the most fundamental reason for flavor characteristics formation in HT. Furthermore, the pollution risks of 5 heavy metals and 50 pesticide residues in HT were less than 1. The complex ecosystem and low chemical control level were speculated to be the primary reasons for the high safety quality of HT. Overall, these findings provide a more comprehensive understanding of quality characteristics and their formation mechanisms in HT.
Edible hyaluronic acid‐rich burger separator discs prepared from slaughterhouse waste
Authors: Kalantarmahdavi Mahboubeh , Salari Amir , Pasdar Zahra , Amiryousefi Mohamad Reza 
Published: June 2022

AbstractIn this study, edible films from chicken feet extract (CF), ovine muscle fascia extract (MF), and bovine bone gelatin powder (Gel) were prepared and their characteristics were analyzed. We also used the films as separators of burger cuts and evaluated the organoleptic characteristics of cooked burgers. Hyaluronic acid quantities of CF and MF were measured using colorimetric and spectrophotometry. Results indicated that the concentration of hyaluronic acid in CF (124.11 ppm) was greater than MF (101.11 ppm). The antioxidative property of the CF film (18.47%) was greater than the Gel (1.88%) and MF (Undetectable) films. The CF film was more resistant to water vapor permeability (2.75 × 10−9 g/m.s.pa) than the MF (1.57 × 10−8 g/m.s.pa) and Gel (1.5 × 10−7 g/m.s.pa) films. The Gel film had more appropriate mechanical properties than CF and MF films. The films kept burgers patties independent from one another and prevented them from sticking and freezing together. MF and CF films were able to promote the organoleptic properties of cooked burgers in taste and texture.
Dietary aluminium intake disrupts the overall structure of gut microbiota in Wistar rats
Authors: Wang Bo , Wu Caihong , Cui Lianzhi , Wang Hui , Liu Ya , Cui Weiwei 
Published: June 2022

AbstractApproximately, 40% of ingested dietary aluminium accumulates in the intestine, which has been considered a target organ for dietary aluminium exposure. The gut microbiota may be the first protective barrier against the toxic metal aluminium and a crucial mediator of the bioavailability of metal aluminium. We previously evaluated dietary aluminium intake and its health risks in a population from Jilin Province, China, and found that the average daily intake of aluminium in the diet of residents in Jilin Province was 0.163 mg/kg after the total diet survey. In the present study, the equivalent concentration of aluminium in rats was extrapolated by the average dietary aluminium intake in the population of Jilin Province based on body surface area. Furthermore, healthy adult Wistar rats were randomly divided into four groups (n = 15 for each group): a control group and three groups treated with aluminium solution (1, 10, and 100 mg/kg/day, intragastrically) for 28 days. Following treatment, necrosis of renal tubular epithelial cells, hyperplasia of bile ducts and hyperplasia of heart tissue, as well as fiber in the liver, kidney, and heart tissues of aluminium‐treated rats were observed, although there were no significant changes in the spleen and brain. Subsequently, fecal samples were withdrawn for 16S rRNA gene sequence analysis. It was found that aluminium decreased the microbiota diversity and changed the overall community structure of the gut microbiota, including three phyla and four genera, together with the regulation of 12 signaling pathways. Collectively, treatment with aluminium markedly altered the structure of the gut microbiota, suggesting that the disorders of intestinal flora induced by aluminium may be an important mechanism for aluminium toxicity.
Evaluating denoising strategies in resting‐state functional magnetic resonance in traumatic brain injury (EpiBioS4Rx)
Authors: Weiler Marina , Casseb Raphael F. , de Campos Brunno M. , Crone Julia S. , Lutkenhoff Evan S. , Vespa Paul M. , Monti Martin M. ,   for the EpiBioS4Rx Study Group
Published: June 2022

AbstractResting‐state functional MRI is increasingly used in the clinical setting and is now included in some diagnostic guidelines for severe brain injury patients. However, to ensure high‐quality data, one should mitigate fMRI‐related noise typical of this population. Therefore, we aimed to evaluate the ability of different preprocessing strategies to mitigate noise‐related signal (i.e., in‐scanner movement and physiological noise) in functional connectivity (FC) of traumatic brain injury (TBI) patients. We applied nine commonly used denoising strategies, combined into 17 pipelines, to 88 TBI patients from the Epilepsy Bioinformatics Study for Anti‐epileptogenic Therapy clinical trial. Pipelines were evaluated by three quality control (QC) metrics across three exclusion regimes based on the participant's head movement profile. While no pipeline eliminated noise effects on FC, some pipelines exhibited relatively high effectiveness depending on the exclusion regime. Once high‐motion participants were excluded, the choice of denoising pipeline becomes secondary ‐ although this strategy leads to substantial data loss. Pipelines combining spike regression with physiological regressors were the best performers, whereas pipelines that used automated data‐driven methods performed comparatively worse. In this study, we report the first large‐scale evaluation of denoising pipelines aimed at reducing noise‐related FC in a clinical population known to be highly susceptible to in‐scanner motion and significant anatomical abnormalities. If resting‐state functional magnetic resonance is to be a successful clinical technique, it is crucial that procedures mitigating the effect of noise be systematically evaluated in the most challenging populations, such as TBI datasets.
Limited disassembly of cytoplasmic hepatitis B virus nucleocapsids restricts viral infection in murine hepatic cells
Authors: Zhao Kaitao , Guo Fangteng , Wang Jingjing , Zhong Youquan , Yi Junzhu , Teng Yan , Xu Zaichao , Zhao Li , Li Aixin , Wang Zichen , Chen Xinwen , Cheng Xiaoming , Xia Yuchen 
Published: June 2022

Background and Aims:
Murine hepatic cells cannot support hepatitis B virus (HBV) infection even with supplemental expression of viral receptor, human sodium taurocholate cotransporting polypeptide (hNTCP). However, the specific restricted step remains elusive. In this study, we aimed to dissect HBV infection process in murine hepatic cells.

Approach and Results:
Cells expressing hNTCP were inoculated with HBV or hepatitis delta virus (HDV). HBV pregenomic RNA (pgRNA), covalently closed circular DNA (cccDNA), and different relaxed circular DNA (rcDNA) intermediates were produced in vitro. The repair process from rcDNA to cccDNA was assayed by in vitro repair experiments and in mouse with hydrodynamic injection. Southern blotting and in situ hybridization were used to detect HBV DNA. HBV, but not its satellite virus HDV, was restricted from productive infection in murine hepatic cells expressing hNTCP. Transfection of HBV pgRNA could establish HBV replication in human, but not in murine, hepatic cells. HBV replication‐competent plasmid, cccDNA, and recombinant cccDNA could support HBV transcription in murine hepatic cells. Different rcDNA intermediates could be repaired to form cccDNA both in vitro and in vivo. In addition, rcDNA could be detected in the nucleus of murine hepatic cells, but cccDNA could not be formed. Interestingly, nuclease sensitivity assay showed that the protein‐linked rcDNA isolated from cytoplasm was completely nuclease resistant in murine, but not in human, hepatic cells.

Conclusions:
Our results imply that the disassembly of cytoplasmic HBV nucleocapsids is restricted in murine hepatic cells. Overcoming this limitation may help to establish an HBV infection mouse model.
Tackling change in mental health service delivery: A qualitative evaluation of a lifestyle program targeting mental health staff – Keeping our Staff in Mind (KoSiM)
Authors: Watkins Andrew , Stein‐Parbury Jane , Curtis Jackie , Poole Josephine , Teasdale Scott , Fibbins Hamish , Rossimel Elisa , Lederman Oscar , Ward Philip B. , Rosenbaum Simon , Denney‐Wilson Elizabeth 
Published: June 2022

AbstractIssues addressedPeople with severe mental illness have adverse health outcomes compared to the general population. Lifestyle interventions are effective in improving health outcomes in this population. Current cultural processes in mental health services do not generally incorporate physical health care practices. Innovative education is required to improve knowledge and confidence of staff in the delivery of preventative health measures.MethodsThe Keeping our Staff in Mind (KoSiM) program delivered a brief lifestyle intervention to mental health staff. A qualitative analysis following the Standards for Reporting Qualitative Research was undertaken. Semi‐structured interviews designed to elicit information about the acceptability of the program and the impact of the intervention on participants' personal and professional lives. The interviews were analysed using thematic analysis, with coding independently developed and reviewed by three authors.ResultsOf the 103 eligible participants, 75 were interviewed. Responses revealed four main themes: (i) positive changes in clinician's approach to physical health care, (ii) improvements in attitudes to self‐care and family wellbeing, (iii) positive changes in workplace culture associated with physical health care delivery and (iv) high levels of acceptability of the program.ConclusionThe KoSiM model may be useful in other settings as a means of changing the culture of mental health services to better integrate physical health care as a core part of mental health service provision.So what?A novel approach using staff focussed lifestyle interventions model may cut through the resistance that is encountered when implementing proven methods of clinical intervention where cultural barriers exist.
Missed nursing care in highly specialized hospitals: A Mexican case study
Authors: Hernández‐Corral Sandra , Serván‐Mori Edson , Benítez‐Chavira Luis A. , Nigenda Gustavo , Zárate‐Grajales Rosa A. 
Published: June 2022

AbstractObjectiveTo assess the status of missed nursing care and the reasons for its occurrence in a highly specialised public hospital in Mexico.Materials and methodsAn observational cross‐sectional analysis with data collected from January to June 2019 at the National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra in Mexico City. We assessed missed care and its causes by conducting the MISSCARE survey among 116 nursing professionals selected from a population of 162 nurses. This work presents the estimated rates of missed care—overall and broken down into its four dimensions—as well as the reasons for its occurrence, namely limited labour resources, inadequate material resources and communication problems among work teams.ResultsThe overall score for missed care was 16% (95% CI: 11.84%–20.15%), with the following rates by dimension: 19.48% for basic care, 14.66% for individual needs, 6.47% for patient education and discharge planning, and 4.31% for continuous patient assessment. The main reason cited for missed care was inadequate material resources, followed by limited labour resources and communication problems among work teams.ConclusionBasic care and individual needs interventions were the most frequently omitted services, primarily because of inadequate material resources, limited labour resources and communication problems among work teams. An increase in the frequency of missed care can be expected in light of the high demand for health services, particularly as regards labour and material resources, imposed by the SARS‐CoV‐2 pandemic.
Trends of adverse events and mortality after DMARDs in patients with rheumatoid arthritis: Interrupted time‐series analysis
Authors: Fang Yao‐Fan , Liu Jia‐Rou , Chang Shu‐Hao , Kuo Chang‐Fu , See Lai‐Chu 
Published: June 2022

AbstractObjectivesPatients with rheumatoid arthritis (RA) experience adverse events because of the characteristics of the disease and the side effects of medications. We investigated the trends of adverse events and mortality associated with disease‐modifying antirheumatic drugs (DMARDs).MethodsWe used the Taiwan National Health Insurance Database to enroll patients with incident RA between 2000 and 2017. The 1‐year incident rate of gastrointestinal (GI) bleeding and 3‐year incident rates of other adverse events and mortality for each calendar‐quarter cohort were computed and adjusted using propensity score‐based stabilized weights for fair comparisons. Levels and trends of the conventional DMARD era (2000–2002, Phase 1) were compared with those of the TNFi era (2003–2012, Phase 2) and OMA era (2013–2017, Phase 3) by using interrupted time series (ITS) analysis.ResultsAll patients with RA were prescribed cDMARDs in Phase 1 (2000–2002), and 1%–3% were prescribed either TNFi in phase 2 (2003–2012) or OMAs in phase 3 (2013–2017). The cancer incidence rate was 1.90%, and its mortality rate was 4.19%. After the introduction of TNFi from 2003 to 2012, the main outcomes, except TKA, exhibited a steady or mild decrease in trends. ITS analysis revealed that the slope mildly increased in 2003–2012 compared with that in 2000–2003 by 0.13% for total knee replacement (p = .0322). In 2012–2017 (the OMA era), the events became steady.ConclusionIn patients with RA, the introduction of DMARDs was associated with stable adverse events and mortality rates. Moreover, the introduced new treatment for RA exhibited a good safety profile.
The prognostic impact of myosteatosis on overall survival in gynecological cancer patients: A meta‐analysis and trial sequential analysis
Authors: Cao Hongyi , Gong Yang , Wang Yue 
Published: June 2022

AbstractMyosteatosis is a novel imaging biomarker for survival in gynecological cancer patients; however, the evidence is inconsistent. This meta‐analysis aims to investigate the impact of myosteatosis on overall survival in the gynecological oncology setting. Three databases (PubMed, EMBASE and Web of Science) were systematically searched for relevant literature up to October 30, 2021. A random‐effects model was used to evaluate the predictive effect of myosteatosis on overall survival in the gynecological cancer population. The Newcastle‐Ottawa Scale was used to assess the methodological quality of the included studies. Trial sequential analysis was used to control the risk of random errors. Twelve studies with a total of 2519 patients were included. Myosteatosis was associated with a 50% increased mortality risk (HR 1.50, 95% CI 1.24‐1.82, P < .001) in gynecological cancer patients. Subgroup analyses stratified by study design, statistical model, treatment, sample size and stage confirmed the predictive value of myosteatosis on survival. However, the prognostic ability of myosteatosis only was held in the American and European populations but lost in Asians. Additionally, myosteatosis was not associated with the increased mortality in endometrial and cervical cancers, except for ovarian cancers. Overall, myosteatosis is a powerful predictor of reduced overall survival in gynecological cancer patients.
Multicenter, single‐arm, phase II study of the continuous use of panitumumab in combination with FOLFIRI after FOLFOX for RAS wild‐type metastatic colorectal cancer: Exploratory sequential examination of acquired mutations in circulating cell‐free DNA
Authors: Watanabe Jun , Maeda Hiromichi , Nagasaka Takeshi , Yokota Mitsuru , Hirata Keiji , Akazawa Naoya , Kagawa Yoshinori , Yamada Takeshi , Shiozawa Manabu , Ando Takayuki , Kato Takeshi , Mishima Hideyuki , Sakamoto Junichi , Oba Koji , Nagata Naoki 
Published: June 2022

AbstractThis multicenter single‐arm, phase II study evaluated the efficacy and safety of uninterrupted panitumumab usage combined with cytotoxic doublets for unresectable/metastatic colorectal cancer (mCRC). Additionally, clinical value of the RAS/BRAF mutation status in circulating cell‐free DNA (ccfDNA) was evaluated; this evaluation was measured independently of the protocol treatment. Eligible patients with RAS wild‐type mCRC who had received the first‐line panitumumab plus FOLFOX treatment were recruited and administered continuous panitumumab combined with FOLFIRI. Progression‐free survival (PFS) at 6 months was the primary endpoint, with threshold and expected values of 35% and 50%, respectively. In total, 54 patients were enrolled between October 2017 and October 2019. The crude 6‐month PFS rate was 37.0%, with a 4.8‐month median PFS. The response rate and disease control rate were 16.7% and 50.0%, respectively. Notably, of the 54 participants, 17 showed RAS/BRAF mutations until the end of the protocol treatment and of the 22 patients with progressive disease as their best response, 10 possessed RAS/BRAF mutations in their plasma ccfDNA at baseline. The median PFS significantly differed among patients harboring tumors with BRAF and RAS mutations and those with wild‐type tumors. In conclusion, our study failed to show the expected efficacy of the continuous panitumumab use in the second‐line treatment. Liquid biopsy discriminated the duration of PFS according to the mutation status. The effectiveness of continuous treatment with panitumumab should be evaluated in patients with RAS/BRAF wild‐type mCRC determined by liquid biopsy at the start of the second‐line treatment.