bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic approach with commercial supplements for pantothenate kinase-associated neurodegeneration with residual PANK2 expression levels.
Authors: Álvarez-Córdoba Mónica , Reche-López Diana , Cilleros-Holgado Paula , Talaverón-Rey Marta , Villalón-García Irene , Povea-Cabello Suleva , Suárez-Rivero Juan M , Suárez-Carrillo Alejandra , Munuera-Cabeza Manuel , Piñero-Pérez Rocío , Alcazar Jose A Sanchez 
Published: June 2022

Abstract
In this manuscript, we examined the therapeutic effectiveness of pantothenate, panthetine, antioxidants (vitamin E and omega 3) and mitochondrial function boosting supplements (L-carnitine and thiamine) in mutant PANK2 cells with residual expression levels. Results: Commercial supplements, pantothenate, pantethine, vitamin E, omega 3, carnitine and thiamine were able to eliminate iron accumulation, increase PANK2, mtACP, and NFS1 expression levels and improve pathological alterations in mutant cells with residual PANK2 expression levels.Conclusion: Our results suggest that several commercial compounds are indeed able to significantly correct the mutant phenotype in cellular models of PKAN. These compounds alone or in combinations are of common use in clinical practice and may be useful for the treatment of PKAN patients with residual enzyme expression levels.
Cultivated autologous limbal epithelial cells (CALEC): product development, manufacture, and initial evaluation of feasibility
Authors: Jurkunas Ula , Yin Jia , Johns Lynette , Li Sanming , Negre Helene , Shaw Kit , Samarakoon Lassana , Ayala Allison , Kheirkhah Ahmad , Katikireddy Kishore , Gauthier Alex , Tone Stephan Ong , Ellender Stacey , Rodriguez Diego Hernandez , Daley Heather , Dana Reza , Ritz Jerome , Armant Myriam 
Published: June 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Positive Status Disclosure and Sexual Risk Behaviour Change among People Living with HIV in Northern Region, Ghana
Authors: Kabriku Claver Peter , Ansah Edward Wilson , Hagan John Elvis 
Published: June 2022

Abstract
Objective: To investigate sexual behaviour changes adopted by PLHIV on Antiretroviral therapy (ART) in the Northern Region of Ghana. Methods: We employed a cross-sectional survey with a questionnaire to collect data from 900 clients from nine major ART centres within the region. Chi-square and logistics regression analyses were applied to the data. Results: The common safe sexual behaviours adopted by more than 50% of PLHIV on ART included: condom use, reduction in sexual partners, abstinence, reduction in unprotected sex with married/regular partners, and avoidance of new casual sex partners. Also, the fear of others getting to know patients’ HIV positive status (χ2=7.916, p =.005), fear of stigma (χ2=5.201, p =.023), fear of losing family support (χ2= 4.211, p =.040) and not ready to disclose yet (χ2= 4.475, p =.034) were statistically significant predictors of non-disclosure of HIV positive status among the participants. Moreover, change in sexual behaviour is influenced by; “to avoid spreading the disease to others”, [R2 =.043, F (1, 898) =40.237, p <.0005], “to avoid contracting other STIs [R2 =.010, F (1, 898) =8.937, p < .0005], live long [R2 =.038, F (1, 898) =35.816, p < .0005], hide HIV-positive status [R2 =.038, F (1, 898) =35.587, p < .0005], achieve good results from ART treatment [R2 =.005, F (1, 898) =4. 282, p <.05] and live a Godly life” [R2 =.023, F (1, 898) =20. 880, p < .0005]. Conclusion: Although a high self-disclosure rate of HIV positive status was identified, with more participants disclosing either to their spouses or parents, reasons for disclosure and non-disclosure differed from person to person. Future HIV prevention interventions should be tailored to facilitate open communication on disclosure status and positive sexual behaviour changes.
Effects of a series of large flares from a sunspot group eruption on VLF propagation
Authors: Zhao Xiukun , Niu Youtian , Wang Zhe , Li Bei , Yang Sai , Zheng Zhiyuan 
Published: June 2022

Abstract
The sunspot group continues to evolve, causing flares to erupt under the action of the atmospheric magnetic field. After the flare erupts, the ejected X-rays cause the electron concentration in the ionosphere to increase, and the equivalent reflection height of the ionosphere decreases. We investigate the correlation between the series of flares erupted by sunspot groups and the propagation of VLF signals. We used NOAA sunspot activity area data and solar flare data released by GOSE satellite, counted the series of flares erupted by sunspot group in 2000, and analyzed the size of the flare. Among them, the sunspot group numbered AR9077 erupted continuously with 15 M-level and above flares, X Level 3 flares. Received data from the Alpha VLF navigation station in Haikou, and observed a series of large flares erupted by AR9077. After the flare disk erupted, the phase anomaly of the VLF signal in the ground-ionosphere was caused. Analyze the flare according to the phase anomaly change, calculate the phase velocity when the flare occurs, the equivalent reflection height change, and the X-ray flux to predict the size of the flare. The calculations are consistent with the comparison of data published by the GOSE satellite. The observations show that the VLF signal correlates well with solar flares and sunspots. The VLF signal can be used as a reliable solution for predicting sunspots and solar flares, avoiding the impact of continuous flares of sunspots.
Whole-exome sequencing identified compound heterozygous variants in DNAH5 in a Chinese pedigree with idiopathic hypogonadotropic hypogonadism
Authors: Tong Lulu , He Meinan , Xiong Fu , Jia Bei , Cao Ying 
Published: June 2022

Abstract
Purpose: This study was designed to identify the underlying molecular genetic cause of idiopathic hypogonadotropic hypogonadism (IHH) in a nonconsanguineous Chinese family. Methods: All the family members underwent medical history evaluation, physical examination, and laboratory studies. Whole-exome sequencing and RNA sequencing was performed on 2 affected siblings and unaffected parents. All candidate variants were confirmed in all family members by Sanger sequencing and silico function prediction. Results: The proband and his twin brother were diagnosed with IHH, and an autosomal recessive mode of inheritance was identified. Whole-exome sequencing identified the compound heterozygous variants c.9230G>A (p.Arg3077Gln) and c.12883G>A (p.Val4295Met) in DNAH5 in both affected siblings. Sanger sequencing verified that c.9230G>A in DNAH5 was from the unaffected mother and c.12883G>A was from the unaffected father. In addition, we found heterozygous mutations in BBS2 (c.1705delC, p.Gln569fs) and NR5A1 (c.460G>A, p.Ala154Thr) in the siblings, which were from their father.Conclusion: Compound heterozygous variants in DNAH5 were identified in the affected siblings, and were predicted to be pathogenic by silico analysis. This is the first report that suggests variants in DNAH5 are relevant to the pathogenesis of IHH. This study expands the variant spectrum of genes associated with IHH.
Gravity Study of the Crust beneath the Babouri-Figuil and Mayo Oulo-Léré Sedimentary Basins, North Cameroon and South Chad
Authors: Saidou Bouba , Bouba Apollinaire , Oyoa Valentin , Yap Loudi , Kamguia Joseph , Manguelle-Dicoum Eliezer 
Published: June 2022

Abstract
In this work, the crustal structure study of Babouri-Figuil and Mayo Oulo-Léré sedimentary basins was carried out through the interpretation of gravity data. These data were obtained by combining to the terrestrial gravity data those obtained from EGM2008 model. The Analysis of the terrestrial Bouguer anomaly maps revealed negative and positive anomalies. Negative anomalies would be the signature of sedimentary basins while positive anomalies would be attributed to basaltic rocks under granitic environment. To isolate anomalies due to deep structures from those due to near surface structures, we used the empirical method. This method testifies that the residual map of order 4 is an appropriate one. In order to conduct the quantitative interpretation of the combined gravity data, six profiles were drawn on the residual Bouguer anomaly map and therefore were interpreted using spectral analysis and 2.5D modeling methods. The results indicate that the mean depths of mass sources near surface of Babouri-Figuil and Mayo Oulo-Léré sedimentary basins were 1.50km and 1.55km respectively. Moreover, Babouri-Figuil is constituted of two formations of different density contrast while Mayo Oulo-Léré shows three formations. These models helped us to clarify the geological structure of the study area. The results of the present study allow greater understanding the sedimentary basin thickness.
The multiple facets of mitochondrial regulations controlling cellular thermogenesis.
Authors: Beignon Florian , Gueguen Naig , Tricoire-Leignel Hélène , Mattei César , Lenaers Guy 
Published: June 2022

Abstract
Understanding temperature production and regulation in endotherm organisms becomes a crucial challenge facing the increased frequency and intensity of heat strokes related to global warming. Mitochondria, located at the crossroad of metabolism, respiration, Ca2+ homeostasis and apoptosis, were recently proposed to further act as cellular radiators, with an estimated inner temperature reaching 50°C in common cell lines. This inner thermogenesis might be further exacerbated in organs devoted to produce consistent efforts as muscles, or heat as brown adipose tissue, in response to acute solicitations. Consequently, pathways promoting respiratory chain uncoupling and mitochondrial activity, such as Ca2+ fluxes, uncoupling proteins, futile cycling and substrate supplies, provide the main processes controlling heat production and cell temperature. The mitochondrial thermogenesis might be further amplified by cytoplasmic mechanisms promoting the over-consumption of ATP pools. Considering these new thermic paradigms, we discuss here all conventional wisdoms linking mitochondrial functions to cellular thermogenesis in different physiological conditions.
MicroRNA-34b/c is involved in the response of human liver cells to the Corylus avellana L. hazelnut extract
Authors: benassi barbara , Bacchetta Loretta , Pacchierotti Francesca 
Published: June 2022

Abstract
Background Epigenetic regulation by microRNAs (miRs) underlies liver tissue biology and affects the hepatocytes response to pathogens, drugs, and food-derived molecules.Methods and results In this study we characterized the epigenetic role driven by an ethanolic extract of hazelnut (Corylus Avellana L, HZN) in both THLE-2 human primary hepatocytes and HepG2 human hepatocarcinoma cells (HCC), in terms of microRNA-34b/c involvement. Both the precursor transcript and mature molecules (miR-34b and miR-34c) were found hyper-expressed in primary cells if compared to HCC (P<0.05). When treated with HZN (0.04-0.4 mg/ml) for 72h, microRNA-34b/c underwent significant stimulation (≥ 2-fold change, P<0.05) exclusively in primary hepatocytes, whereas 144h of continuous HZN treatment were required to induce such an increase in HCC, demonstrating a different kinetics of miR-34 stimulation in liver cells, according to their either cancer or non-cancer phenotype. In THLE-2 cells, miR-34b/c stimulation plays a significant antioxidant role; when challenged with oxygen peroxide (H2O2 1000-2000 µM, 24h), cells are significantly protected from oxidative stress if pre-treated with HZN, the H2O2-driven cytotoxicity and reactive oxygen species generation being recovered by hazelnut. In HCC cells, prolonged exposure (144h) to high HZN concentration (0.4 mg/ml) triggers cell growth arrest and cell death, demonstrating a promising potential anti-neoplastic function of the biomolecules composing the hazelnut extract.Conclusions Overall, our experimental findings demonstrate the epigenomic effect of the Corylus Avellana L. extract in human liver cells in vitro, highlighting the great potential of tree nuts consumption for personalized nutrition and of its bioactive molecules for possible therapeutic options.
Effect of braking torque on vehicle nonlinear dynamics
Authors: Wang Xianbin , Li Weifeng , Li Zhipeng , Li Zexuan , Zhang Fugang 
Published: June 2022

Abstract
To analyze the effect of braking torque on vehicle nonlinear dynamic characteristics, a five-degree-of-freedom(5DOF) vehicle nonlinear dynamic model including braking torque is established in this paper. The global dynamic characteristics of the model are analyzed through the phase space of multiple initial points. The effect of constant braking torque on vehicle dynamic characteristics is studied by time-domain analysis and tire force distribution characteristics of a single initial point. The characteristics of bifurcation and chaos are analyzed by using the differences in trajectories in phase space, the bifurcation diagram of state variables and the Lyapunov exponent spectrum. The results show that the braking torque has a significant impact on the vehicle’s nonlinear dynamic characteristics; With the increase of the braking torque, the five-degree-of-freedom model system may occur multiple bifurcations and hyperchaos under the condition of the zero-steering angle of the front wheel; some instable vehicle dynamic states under zero front-wheel steering angle can only be restored to a stable state with the action of braking torque in a certain range of values.
Generation of far-infrared emission longer than 100 μm by inter-Rydberg transitions of NO
Authors: Hoshino Shoma , Nishimura Kento , Abe Rin , Tsukiyama Koichi 
Published: June 2022

Abstract
We report generation of directional far-infrared emission between Rydberg states (s, p, f, and g Rydberg series) of NO in the gas phase initiated by the absorption of black body radiation at room temperature. All the transitions detected are assignable to the nf → (n – 1)g and n'g → (n' – 1)f transitions. The longest wavelength observed in the present experiment was ~ 112 µm (2.7 THz) which corresponds to the 14f → 13g transition. The laser-prepared population in the 14s and 15s states are transferred to the 13f and 14f states located energetically higher, respectively, which generates the subsequent emission of 13f → 14g at ~ 88 µm and 14f → 13g at ~ 112 µm. Collisions of Rydberg molecules with the surrounding ground state molecules are considered to responsible for the upward population flow. The population in the 14p and 15p states are transported to the 13f and 14f states located energetically lower, respectively, which gives rise to the subsequent emission of 13f → 12g at ~ 88 µm and 14f → 13g at ~ 112 µm.
Research on the Dynamic Behaviors in the Sliding Friction of Silicone
Authors: Gao Yunlong , Wang Yongjuan 
Published: June 2022

Abstract
With the promotion of soft viscoelastic materials in the field of flexible bionic robots, the behavior of sliding friction between soft viscoelastic materials and rigid materials such as metals has attracted great attention. In this study, the research object was silicone, and a research method combining a dynamic force testing technique and a non-contact optical testing technique for deformation and strain was proposed to study the frictional behavior of silicone and the effect of the tooth structure surface on the sliding friction. we analyzed the temporal changes in the friction force for different loads, the dynamic characteristics of large deformation and micro-strain on the front side (define the contact surface as the bottom surface) of the silicone. The results showed that within a certain range, the sliding friction coefficient of the silicone varies linearly with the load, and there were stick-slip and deformation waves during the relative motion. The larger the load was, the faster the deformation wave propagated, and the tooth structure surface had sliding friction anisotropy.
Quantitative assessment method of muzzle flash and smoke at high noise level on field environment
Authors: Yan Chenguang , Zhu Chenguang 
Published: June 2022

Abstract
It is quite a challenge to obtain the temperature and species concentration fields of muzzle flash at high noise level. In this numerical study, radiation intensity of muzzle flash received by the high-speed Complementary Metal-Oxide-Semiconductor (CMOS) camera was simulated based on the line-of-sight method in the direct radiative transfer problem. The inverse radiative transfer problem of reconstructing distributions of temperature and soot volume fraction from the knowledge of flame radiation intensity was transformed into a minimization optimization problem and a meta-heuristic algorithm was used to solve the problem. The effects of the number of detection lines, optical thickness and measurement errors on the reconstruction results were discussed in details. A method to estimate the noise level of radiation intensity was developed, experimental results showed that the signal-to-noise ratio (SNR) of radiation intensity can be successfully inferred when the SNR is greater than 20 dB. Subsequently, prior knowledge of the noise level was introduced in the regularization to achieve a meaningful approximation of the exact value. The reconstruction of the soot volume fraction filed with SNR greater than 40 dB is considered successful with the inclusion of an appropriate regularization term in the objective function, and the reconstruction of the temperature field is feasible even with SNR as low as 15 dB. The high tolerance to the noise level of the radiation intensity gives the reconstruction algorithm the potential to be used in practical experiments of muzzle flash.
Levels of Interleukin-35 changes in Gingival Crevicular Fluid after Non-Surgical Periodontal Therapy in stage III periodontitis: a randomized, controlled clinical Trial
Authors: Jadhav Ashwini , Rathod Surekha , Kolte Abhay 
Published: June 2022

Abstract
Objectives This parallel, randomized controlled clinical trial evaluated the levels of IL-35 in periodontally healthy, gingivitis and periodontitis patients before and after the Non-Surgical Periodontal Therapy (NSPT). Methods: This trial was registered in Clinical trial registry-India (ref no.-REF/2020/03/032301). 60 patients included in the study with an equal allotment ratio for 3 groups of periodontally healthy (Group I), Group II (Gingivitis patients) and Group III (Stage III Periodontitis patients) were evaluated for clinical and biochemical parameters in GCF for levels of IL-35 through enzyme linked immunosorbent assay (ELISA). The patients were subjected to NSPT and the 3 months post therapy levels were examined.Results: Group III exhibited higher values of PPD (5.8500 mm), CAL (7.60), PI (2.8929) while lower PBI (2.1170) and GI (2.1085) scores as compared to Group II. The scores for all these parameters reduced significantly from baseline to 3 months in both the groups after NSPT. IL-35 levels in GCF differed across the groups with minimum values seen for Group III which increased incrementally to Group II and I. Also, the IL-35 levels in GCF significantly reduced from baseline to 3 months amongst Group II and III.Conclusion: The results indicate reduced GCF levels of IL-35 in Group II and III compared to Group I, thereby implying that IL-35 plays a crucial role in the pathogenesis of periodontal diseases.
Efficacy of pemafibrate on triglyceride levels, liver and renal functions compared with femafibrate and bezafibrate in patients with hypertriglyceridemia and type 2 diabetes
Authors: Oya Junko , Nakagami Tomoko , Kondo Yuichiro , Katamine Aki , Shimizu Mika , Hasegawa Yukiko , Babazono Tetsuya 
Published: June 2022

Abstract
Purpose
To evaluate and compare the efficacy and safety of pemafibrate, a selective peroxisome proliferator-activator α modulator, with fenofibrate and bezafibrate, classical fibrates, in a real-world setting in patients with hypertriglyceridemia and type 2 diabetes (T2D).
Methods
200 patients with hypertriglyceridemia (> 175 mg/dL) and T2D were studied. Adjusted least square mean percentage changes in serum levels of triglyceride (TG), transaminases, γ-glutamyl transpeptidase (γGTP), and creatinine kinase (CK) levels, and estimated glomerular filtration rates (eGFRs) were compared before and after 3-month therapy with pemafibrate 0.1 mg and 0.2 mg, fenofibrate 160 mg, and bezafibrate 400 mg. Multivariate logistic regression analyses were performed to examine the association between the fibrate therapy and achievement of TG target levels (< 175 mg/dL) and the deterioration of liver and renal functions.
Results
The pemafibrate therapy exhibited a greater reduction in TG levels of 50%, which was 3–4 times better at achieving the target range for TGs, than fenofibrate. The administration of pemafibrate but not fenofibrate was significantly associated with a lower risk of elevation in γGTP. Similarly, a smaller risk of reduction in eGFR was observed in the pemafibrate groups than in the fenofibrate group.
Conclusion
Pemafibrate therapy achieved a greater reduction in TG levels towards the desired target range than fenofibrate therapy. Moreover, pemafibrate was associated with a lower risk profile for elevated γGTP and reduced eGFR than fenofibrate.
Multimodal Feature Extraction Algorithm for Potential Frequency in the Fusion of EEG and MRI
Authors: Li Xiaofeng , Huang Heyan 
Published: June 2022

Abstract
To address the problem that the traditional Electroencephalogram (EEG) induces insufficient consideration of multimodal in the potential frequency feature algorithm leading to the degradation of data quality, which in turn affects the accuracy of potential frequency feature extraction. Therefore, we propose a potential frequency multimodal feature extraction algorithm in the Fusion of EEG and Magnetic Resonance Imaging (MRI). First, the multimodal EEG was preprocessed. The Empirical mode decomposition was used to decompose the multimodal EEG into multiple Intrinsic Mode Function (IMF) components, extract the principal IMF components, and complete the reconstruction of the multimodal EEG by combining with the EEG frequency calculation. Second, time-frequency reassignment was used to calculate the reconstructed EEG frequency features and complete the coarse extraction of features. Finally, mutual information was used to extract the frequency features most relevant to the induced EEG, to form a new feature set, and to finally realize the potential frequency multimodal feature extraction. Experimental tests were conducted on the BCI2008 competition dataset, DEAP data set, Impedance data set, as well as the stimulation-evoked EEG signal test data set of an individual in a hospital. The results show that the proposed algorithm is characterized by better EEG preprocessing and signal reconstruction effect, strong subspace clustering of frequency features and less spurious frequencies in the feature sequence; the proposed algorithm has the average accuracy of potential frequency feature extraction up to about 95% and the accuracy of EEG signal classification using the extracted features up to about 97%, MRI and EEG signal fusion effect is good, which further verifies the high efficiency of the proposed algorithm and provides a certain theoretical basis for clinical medical research.