bluuebunny/crossref_metadata_2025_split huggingface dataset:
Which mouse multiparental population is right for your study? The Collaborative Cross inbred strains, their F1 hybrids, or the Diversity Outbred population
Authors: Keele Gregory R. 
Published: August 2022

AbstractMultiparental populations (MPPs) encompass greater genetic diversity than traditional experimental crosses of two inbred strains, enabling broader surveys of genetic variation underlying complex traits. Two such mouse MPPs are the Collaborative Cross (CC) inbred panel and the Diversity Outbred (DO) population, which are descended from the same eight inbred strains. Additionally, the F1 intercrosses of CC strains (CC-RIX) have been used and enable study designs with replicate outbred mice. Genetic analyses commonly used by researchers to investigate complex traits in these populations include characterizing how heritable a trait is,i.e., its heritability, and mapping its underlying genetic loci,i.e., its quantitative trait loci (QTLs). Here we evaluate the relative merits of these populations for these tasks through simulation, as well as provide recommendations for performing the quantitative genetic analyses. We find that sample populations that include replicate animals, as possible with the CC and CC-RIX, provide more efficient and precise estimates of heritability. We report QTL mapping power curves for the CC, CC-RIX, and DO across a range of QTL effect sizes and polygenic backgrounds for samples of 174 and 500 mice. The utility of replicate animals in the CC and CC-RIX for mapping QTLs rapidly decreased as traits became more polygenic. Only large sample populations of 500 DO mice were well-powered to detect smaller effect loci (7.5-10%) for highly complex traits (80% polygenic background). All results were generated with our R package musppr, which we developed to simulate data from these MPPs and evaluate genetic analyses from user-provided genotypes.
Modelling Sex Differences in Circadian Regulation of Kidney Function of the Mouse
Authors: Layton Anita T. , Gumz Michelle L. 
Published: August 2022

AbstractKidney function is regulated by the circadian clock. Not only do glomerular filtration rate (GFR) and urinary excretion oscillate during the day, the expressions of several renal transporter proteins also exhibit circadian rhythms. Interestingly, the circadian regulation of these transporters may be sexually dimorphic. Thus, the goal of this study is to investigate the mechanisms by which kidney function of the mouse is modulated by sex and time of day. To accomplish this, we have developed the first computational models of epithelial water and solute transport along the mouse nephrons that represent the effects of sex and circadian clock on renal hemodynamics and transporter activity. We conduct simulations to study how the circadian control of renal transport genes affects overall kidney function, and how that process differs between male and female mice. Simulation results predict that tubular transport differs substantially among segments, with relative variations in water and Na+ reabsorption along the proximal tubules and thick ascending limb tracking that of GFR. In contrast, relative variations in distal segment transport are much larger, with Na+ reabsorption almost doubling during the active phase. Oscillations in Na+ transport drive K+ transport variations in the opposite direction. Model simulations of BMAL1 knockout mice predict a significant reduction in net Na+ reabsorption along the distal segments in both sexes, but more so in males than females. This can be attributed to the reduction of mean ENaC activity in males only, a sex-specific effect that may lead to a reduction in blood pressure in males.
Molecular phenotyping of single pancreatic islet leader beta cells by “Flash-Seq”
Authors: Chabosseau Pauline , Yong Fiona , Delgadillo-Silva Luis F. , Lee Eun Young , Li Shiying , Gandhi Nidhi , Wastin Jules , Noriega Livia Lopez , Leclerc Isabelle , Ali Yusuf , Hughes Jing W. , Sladek Robert , Martinez-Sanchez Aida , Rutter Guy A. 
Published: August 2022

AbstractAimsSpatially-organised increases in cytosolic Ca2+within pancreatic beta cells in the pancreatic islet underlie the stimulation of insulin secretion by high glucose. Recent data have revealed the existence of subpopulations of beta cells including “leaders” which initiate Ca2+waves. Whether leader cells possess unique molecular features, or localisation, is unknown.Main methodsHigh speed confocal Ca2+imaging was used to identify leader cells and connectivity analysis, running under MATLAB and Python, to identify highly connected “hub” cells. To explore transcriptomic differences between beta cell sub-groups, individual leaders or followers were labelled by photo-activation of the cryptic fluorescent protein PA-mCherry and subjected to single cell RNA sequencing (“Flash-Seq”).Key findingsDistinct Ca2+wave types were identified in individual islets, with leader cells present in 73 % (28 of 38 islets imaged). Scale-free, power law-adherent behaviour was also observed in 29% of islets, though “hub” cells in these islets did not overlap with leaders. Transcripts differentially expressed (295; padj<0.05) between leader and follower cells included genes involved in cilium biogenesis and transcriptional regulation. Functionally validating these findings, cilia number and length tended to be lower in leadervsfollower cells. Leader cells were also located significantly closer to delta cells in Euclidian space than were follower cells.SignificanceThe existence of both a discrete transcriptome and unique localisation implies a role for these features in defining the specialized function of leaders. Specifically, these data raise the possibility of altered signalling from delta cells towards somatostatin receptors present on leader cell cilia.
A method for chronic and semi-chronic microelectrode array implantation in deep brain structures using image guided neuronavigation
Authors: Mahmoudian Borna , Dalal Hitarth , Lau Jonathan , Corrigan Benjamin , Barker Kevin , Rankin Adam , Chen Elvis C.S. , Peters Terry , Martinez-Trujillo Julio C. 
Published: August 2022

AbstractPrecise targeting of deep brain structures in humans and large animal models has been a challenge for neuroscientists. Conventional protocols used in animal models typically require large access chambers which are prone to infection and involve assembly and implantation of complex microdrives for semi-chronic applications. Here we present a methodology for improving targeting of subcortical structures in large animals such as macaque monkeys, using image guided neuronavigation. Design of custom cranial caps allowed for incorporation of stable fiducial markers, required for increased targeting accuracy in neuronavigation procedures, resulting in an average targeting error of 1.6 mm over three implantations. Incorporation of anchor bolt chambers, commonly used in human neurosurgery, provided a minimally invasive entrance to the brain parenchyma, allowing for chronic recordings. By leveraging existing 3D printing technology, we fabricated an anchor bolt-mounted microdrive for semi-chronic applications. Our protocol leverages commercially available tools for implantation, decreases the risk of infection and complications of open craniotomies, and improves the accuracy and precision of chronic electrode implantations targeting deep brain structures in large animal models.
When working from home might come at a cost: the relationship between family boundary permeability, overwork climate and exhaustion
Authors: Lazauskaite-Zabielske Jurgita , Ziedelis Arunas , Urbanaviciute Ieva 
Published: August 2022

PurposeDrawing on boundary theory, this study aims to analyse whether the intensity of working from home will be related to higher exhaustion through family boundary permeability, with this relationship being more robust when overwork climate is high.Design/methodology/approachIn this paper eight hundred eighty-three white-collar employees working from home during the COVID-19 pandemic in Lithuania were surveyed online.FindingsThe results confirmed the hypotheses, demonstrating that family boundary permeability may explain the relationship between telework intensity and exhaustion. Furthermore, the mediating relationship between the intensity of working from home, family boundary permeability and exhaustion were moderated by overwork climate. Employees who felt the pressure to overwork were more likely to have a more permeable family boundary when working from home and appeared to experience a much higher psychological cost in terms of emotional exhaustion.Originality/valueThe study provides an insight into the relationships between the intensity of working from home, boundary permeability and exhaustion and their boundary conditions when working from home during the COVID-19 pandemic.
Flashover of insulation materials induced by electrostatic electromagnetic pulse
Authors: Zhang Yan , Ge Xianghu , Zhao Xin , Yang Xiaorui , Liu Shanghe , Xuan Jingjing 
Published: August 2022

Purpose
The purpose of this paper is to research the induced flashover laws of different insulation materials under electrostatic electromagnetic pulse, and the induced flashover characteristics of different electrode structures are further explored.

Design/methodology/approach
According to standard IEC 61000–4-2, an experimental system of electrostatic electromagnetic pulse flashover for insulation materials is established. The induction flashover laws of polytetrafluoroethylene, epoxy resin and polymethyl methacrylate surface-mounted finger electrodes under the different intensity of electrostatic electromagnetic pulse are researched. The influence of the finger electrode, needle–needle electrode and needle–plate electrode on insulation flashover was compared. Secondary electron emission avalanche (SEEA) and field superposition theory are used to analyze the experimental results of electrostatic electromagnetic pulse induced flashover.

Findings
The larger the dielectric strength of insulation materials, the more difficult flashover occurs on the surface. The field superposition enhances collision ionization between electrons and gas molecules, which leads to the insulation materials surface induced flashover easily by electrostatic electromagnetic pulse. The sharper the electrode shapes on the insulation materials surface, the stronger the electric field intensity at the cathode triple junction, more initial electrons are excited to form the discharge channel, which easily leads to flashover on the surface of the insulating material.

Originality/value
The proposed field superposition combined with the SEEA method provides a new study perspective and enables a more rational, comprehensive analysis of electrostatic electromagnetic pulse induced flashover of insulation materials. The work of this paper can provide a reference for the safety protection of spacecraft in orbit under a strong electromagnetic field environment, increase the service life of spacecraft and improve the reliability of spacecraft’s safe operation in orbit. It provides a basis for the selection of insulation materials for equipment under the different intensities of the external electromagnetic environment.
Role transitions at the prosumer level: spillover effects in the collaborative economy from an interactive marketing perspective
Authors: Ertz Myriam , Boily Émilie , Sun Shouheng , Sarigöllü Emine 
Published: August 2022

Purpose
The purpose of this study is to examine the process underlying how consumers shift roles from users to suppliers of goods or services in the collaborative economy (CE). It examines quantatively the impact of a series of explanatory variables underlying that switchover process.

Design/methodology/approach
This study identifies and tests the key factors that motivate the user-provider transition by introducing the spillover effect from the proenvironmental literature into collaborative practices and using four experimental designs. Considering behavioral characteristics, context, intrinsic variables and socialization, this study provides an in-depth understanding of the process of transition from user to supplier in the CE.

Findings
The results suggest the interactive nature of the spillover as peer influence boosts changes in individual motivations, preferences and behaviors. Furthermore, promoting solidarity between members of the CE platform facilitates the transition of participants from users to providers. In addition, the users’ perception of socialization, satisfaction and sense of indebtedness may also play a significant role in the transition.

Research limitations/implications
The study highlights the process underlying the switchover from user to provider at the prosumer level. More specifically, this study identifies key variables influencing the intention to switchover in the CE by drawing on the spillover effect from pro-environmental behavior and considering the spillover as an interactive process.

Practical implications
Managers who wish to develop collaborative systems must attract a critical mass of providers to ensure the viability of their systems. Instead of recruiting new providers, managers may convert existing users into providers. This study identifies the key variables to modulate to this end.

Originality/value
The findings offer important managerial implications and shed new light on the CE literature.
A stakeholder perspective on risk and safety planning in a major sporting event
Authors: Børve Hege Eggen , Thøring Thor Atle 
Published: August 2022

PurposeThis study analyses the impact of institutional logics on the outcomes of a sporting event's risk process.Design/methodology/approachThe research design is an exploratory case study of the 2017 Road World Cycling Championship using a qualitative approach. By drawing on multiple sources, documents and experiences of six key stakeholders the richness of the qualitative data were utilised to provide a deeper understanding of the impact of institutional logics on the process.FindingsStakeholders relied on different organisational and professional logics in arguments for certain risk preferences and strategies. Further, the logics behind stakeholder attention varied between stakeholder groups and within a stakeholder group. This led to a tension-filled relationship amongst stakeholders working in the same organisation and between those in different organisations. Finally, new professional practices emerged; however, there were also unintended consequences, such as a large financial deficit, with a significant part of the cost overrun being the risk assessment work.Research limitations/implicationsThe study includes a case study of a major sporting event that was arranged some years ago. Events are usually organised as temporary projects in collaboration with public sector organisations and must be arranged in accordance with the logics of efficiency as well as meeting the particular needs of citizens. Assuming similar risk planning processes may occur in other contexts is reasonable.Originality/valueThe study contributes to knowledge about the impact of logics on the outcome of risk planning and improves scholars' understanding of risk management and the outcomes of planning mega-sporting events. Moreover, insights into these micro-level processes reveal the importance of dealing with the logics that lie beneath stakeholders' perceptions of risk in order to collaborate, achieve common goals, and prevent cost overruns.
“Hope dies, action begins?” The role of hope for proactive sustainability engagement among university students
Authors: Vandaele Mathilde , Stålhammar Sanna 
Published: August 2022

Purpose
Education in sustainability science is largely ignorant of the implications of the environmental crisis on inner dimensions, including mindsets, beliefs, values and worldviews. Increased awareness of the acuteness and severity of the environmental and climate crisis has caused a contemporary spread of hopelessness among younger generations. This calls for a better understanding of potential generative forces of hope in the face of climate change. This paper aims to uncover strategies for fostering constructive hope among students.

Design/methodology/approach
This study examines, through qualitative interviews, the characteristics of constructive hope amongst proactive students enrolled in university programs related to global environmental challenges. Constructive hope describes a form of hope leading to sustained emotional stability and proactive engagement through both individual and collective actions.

Findings
The findings are presented according to four characteristics of constructive hope: goal, pathway thinking, agency thinking and emotional reinforcement. This shows how students perceive the importance of: collaboratively constructing and empowering locally grounded objectives; reinforcing trust in the collective potential and external actors; raising students’ perceived self-efficacy through practical applications; teaching different coping strategies related to the emotional consequences of education on students’ well-being.

Originality/value
We outline practical recommendations for educational environments to encourage and develop constructive hope at multiple levels of university education, including structures, programs, courses and among students’ interactions. We call for practitioners to connect theoretical learning and curriculum content with practice, provide space for emotional expressions, release the pressure from climate anxiety, and to foster a stronger sense of community among students.
The verbiage in variable message signs and traffic diversion during crash incidents
Authors: Acharya Sailesh , Mekker Michelle 
Published: August 2022

Purpose
WIth limited research on the effects of variable message sign (VMS) message content and verbiage on revealed driver behavior, this study aims to investigate how different verbiage of crash-related messages are related to the diversion rate.

Design/methodology/approach
Using ordered logit models, the associations of message verbiage with diversion rates during crash incidents were assessed using five years of VMS message history within a section of I-15 in the state of Utah.

Findings
A significant impact of message verbiage on the diversion rate was observed. Based on the analysis results, the crash message verbiage with the highest diversion was found to be miles to crash + “prepare to stop,” followed by crash location + delay information, miles to crash + “use caution” + lane of the crash, etc. In addition, the diversion rate was found to be correlated to some roadway characteristics (e.g. occupancy in mainline, weather condition and light condition) along with the temporal variations.

Research limitations/implications
These findings could be used by transportation agencies (e.g. state department of transportation [DOTs]) to make informed decisions about choosing the message verbiage during future crash incidents. This study also revealed that higher diversion rates are associated with a shorter distance between the crash location and VMS device location, recommending increasing the number of VMS devices, particularly in crash-prone areas.
Fuzzy gray clustering evaluation of green building operation effect: a case study of Shenzhen Bay One, China
Authors: Zhang Jingqi , Zhao Hui , Guo Ziliang 
Published: August 2022

PurposeThis paper improves the evaluation index system of green building operation effect and establishes the evaluation model of green building operation effect. It is expected to promote energy saving and emission reduction and provide a more scientific evaluation method for green building operation effect evaluation.Design/methodology/approachFirst, 20 key evaluation indexes are selected to establish the operation effective evaluation index system. Then, the combined weight method is proposed to determine the weight of each evaluation index. Next, the gray clustering-fuzzy comprehensive evaluation method is used to construct the green building operation effective evaluation model. Finally, the feasibility and validity of the selected model were verified by taking Shenzhen Bay One green building in Shenzhen as an example.FindingsThis paper establishes the evaluation system of green building operational effect, and evaluates green building from the angle of operational effect. Taking Shenzhen Bay One project as an example, the rationality and applicability of the model are verified.Originality/valueIn this paper, for the first time, relevant indexes of user experience are included in the evaluation system of green building operational effect, which makes the evaluation system more perfect. In addition, a more scientific fuzzy gray clustering method is used to evaluate the operational effect of green building, and a new evaluation model is established.
The predator problem and PCR primers in molecular dietary analysis: Swamped or silenced; depth or breadth?
Authors: Cuff Jordan P. , Kitson James J. N. , Hemprich‐Bennett David , Tercel Maximillian P. T. G. , Browett Samuel S. , Evans Darren M. 
Published: August 2022

AbstractDietary metabarcoding has vastly improved our ability to analyse the diets of animals, but it is hampered by a plethora of technical limitations including potentially reduced data output due to the disproportionate amplification of the DNA of the focal predator, here termed “the predator problem”. We review the various methods commonly used to overcome this problem, from deeper sequencing to exclusion of predator DNA during PCR, and how they may interfere with increasingly common multipredator‐taxon studies. We suggest that multiprimer approaches with an emphasis on achieving both depth and breadth of prey detections may overcome the issue to some extent, although multitaxon studies require further consideration, as highlighted by an empirical example. We also review several alternative methods for reducing the prevalence of predator DNA that are conceptually promising but require additional empirical examination. The predator problem is a key constraint on molecular dietary analyses but, through this synthesis, we hope to guide researchers in overcoming this in an effective and pragmatic way.
A juvenile climbing exercise establishes a muscle memory boosting the effects of exercise in adult rats
Authors: Eftestøl Einar , Ochi Eisuke , Juvkam Inga S. , Hansson Kenth‐Arne , Gundersen Kristian 
Published: August 2022

AbstractAimInvestigate whether juvenile exercise could induce a long‐term muscle memory, boosting the effects of exercise in adults.MethodsWe devised a 5‐week climbing exercise scheme with food reward administered to male juvenile rats (post‐natal week 4–9). Subsequently, the animals were subjected to 10 weeks of detraining (week 9–19) without climbing and finally retraining during week 19–21.ResultsThe juvenile exercise increased fiber cross‐sectional area (fCSA) by 21% (p = 0.0035), boosted nuclear accretion by 13% (p = 0.057), and reduced intraperitoneal fat content by 28% (p = 0.007) and body weight by 9% (p = 0.001). During detraining, the fCSA became similar in the animals that had been climbing compared to naive controls, but the elevated number of myonuclei induced by the climbing were maintained (15%, p = 0.033). When the naive rats were subjected to 2 weeks of adult exercise there was little effect on fCSA, while the previously trained rats displayed an increase of 19% (p = 0.0007). Similarly, when the rats were subjected to unilateral surgical overload in lieu of the adult climbing exercise, the increase in fCSA was 20% (p = 0.0039) in the climbing group, while there was no significant increase in naive rats when comparing to the contralateral leg.ConclusionThis demonstrates that juvenile exercise can establish a muscle memory boosting the effects of adult exercise. The juvenile climbing exercise with food reward also led to leaner animals with lower body weight. These differences were to some extent maintained throughout the adult detraining period in spite of all animals being fed ad libitum, indicating a form of body weight memory.