bluuebunny/crossref_metadata_2025_split huggingface dataset:
Annual HIV screening rates for HIV-negative men who have sex with men in primary care
Authors: Spensley Courtney B. , Plegue Melissa , Seda Robinson , Harper Diane M. 
Published: July 2022

Background
Men who have sex with men (MSM) account for most new HIV diagnoses in the US. Annual HIV testing is recommended for sexually active MSM if HIV status is negative or unknown. Our primary study aim was to determine annual HIV screening rates in primary care across multiple years for HIV-negative MSM to estimate compliance with guidelines. A secondary exploratory endpoint was to document rates for non-MSM in primary care.

Methods
We conducted a three-year retrospective cohort study, analyzing data from electronic medical records of HIV-negative men aged 18 to 45 years in primary care at a large academic health system using inferential and logistic regression modeling.

Results
Of 17,841 men, 730 (4.1%) indicated that they had a male partner during the study period. MSM were screened at higher rates annually than non-MSM (about 38% vs. 9%, p<0.001). Younger patients (p-value<0.001) and patients with an internal medicine primary care provider (p-value<0.001) were more likely to have an HIV test ordered in both groups. For all categories of race and self-reported illegal drug use, MSM patients had higher odds of HIV test orders than non-MSM patients. Race and drug use did not have a significant effect on HIV orders in the MSM group. Among non-MSM, Black patients had higher odds of being tested than both White and Asian patients regardless of drug use.

Conclusions
While MSM are screened for HIV at higher rates than non-MSM, overall screening rates remain lower than desired, particularly for older patients and patients with a family medicine or pediatric PCP. Targeted interventions to improve HIV screening rates for MSM in primary care are discussed.
The PreQ-20 TRIAL: A prospective cohort study of the oncologic safety, quality of life and cosmetic outcomes of patients undergoing prepectoral breast reconstruction
Authors: Acea-Nebril Benigno , García-Novoa Alejandra , García Jiménez Lourdes 
Published: July 2022

Background
Mastectomy currently constitutes a necessary surgical procedure in the oncologic setting and in the context of high risk. Prepectoral breast reconstruction (PBR) has been proposed as a surgical alternative to retropectoral techniques by providing less postoperative morbidity and a better cosmetic result. However, there is a lack of prospective studies that have evaluated its safety and patient-reported satisfaction.

Methods
We conducted a prospective cohort study to assess the safety, quality of life and cosmetic sequelae of PBR in women with breast cancer and high risk. The study’s main objective is to assess the safety of PBR in terms of postsurgical complications and the feasibility of reconstruction (loss of implants). The secondary objectives are to evaluate oncologic safety (local relapses, residual glandular tissue) and to identify factors related to quality of life and cosmetic sequelae. The evaluation of residual tissue will be conducted by MRI 12 to 18 months after the surgery, and the quality-of-life assessment will be performed using the Breast-Q questionnaire. An initial patient evaluation will be conducted 12–18 months after the surgery, and a second evaluation will be performed at 5 years. The estimated sample size is 81 patients.

Discussion
The PreQ-20 study will analyze the impact of PBR on 3 separate measures: safety, quality of life and cosmetic sequelae. Unlike other studies that analyzed these three measures jointly for women with breast cancer and high risk, this study will individualize the results for these 2 patient groups. This differentiation is necessary from the methodological point of view, given that the 2 patient groups have separate clinical and emotional implications. The assessment of these groups will focus on the following aspects: postoperative complications, local relapses, evaluation of residual glandular tissue and incidence rate of primary tumors in the same, the cosmetic sequelae and the satisfaction and the quality-of-life assessment by the patients.

Trial registration
ClinicalTrials.gov: NCT04642508.
A randomized control trial of high-dose micronutrient-antioxidant supplementation in healthy persons with untreated HIV infection
Authors: Wobeser Wendy L. , McBane Joanne E. , Balfour Louise , Conway Brian , Gill M. John , Huff Harold , Kilby Donald L. P. , Fergusson Dean A. , Mallick Ranjeeta , Mills Edward J. , Muldoon Katherine A. , Rachlis Anita , Ralph Edward D. , Rosenes Ron , Singer Joel , Singhal Neera , Tan Darrell , Tremblay Nancy , Vo Dong , Walmsley Sharon L. , Cameron D. William ,   for the MAINTAIN Study Group
Published: July 2022

Background
Although micronutrient and antioxidant supplementation are widely used by persons with human immunodeficiency virus (HIV), a therapeutic role beyond recommended daily allowances (RDA) remains unproven. An oral high-dose micronutrient and antioxidant supplement (Treatment) was compared to an RDA supplement (Control) for time to progressive immunodeficiency or initiation of antiretroviral therapy (ART) in people living with HIV (PLWH).

Methods
This study was a randomized, double-blind, placebo-controlled multicenter clinical trial. PLWH were recruited from Canadian HIV Trials Network sites, and followed quarterly for two years. Eligible participants were asymptomatic, antiretroviral treatment (ART)-naïve, HIV-seropositive adults with a CD4 T lymphocyte count (CD4 count) between 375–750 cells/μL. Participants were randomly allocated 1:1 to receive Treatment or Control supplements. The primary outcome was a composite of time-to-first of confirmed CD4 count below 350 cells/μL, initiation of ART, AIDS-defining illness or death. Primary analysis was by intention-to-treat. Secondary outcomes included CD4 count trajectory from baseline to ART initiation or two years. A Data and Safety Monitoring Board reviewed the study for safety, recruitment and protocol adherence every six months.

Results
Of 171 enrolled participants: 66 (38.6%) experienced a primary outcome: 27 reached a CD4 count below 350 cells/μL, and 57 started ART. There was no significant difference in time-to-first outcome between groups (Hazard Ratio = 1.05; 95%CI: 0.65, 1.70), or in time to any component outcome. Using intent-to-treat censoring, mean annualized rates of CD4 count decline were -42.703 cells/μL and -79.763 cells/μL for Treatment and Control groups, with no statistical difference in the mean change between groups (-37.06 cells/μL/52 weeks, 95%CI: (-93.59, 19.47); p = 0.1993). Accrual was stopped at 171 of the 212 intended participants after an interim analysis for futility, although participant follow-up was completed.

Conclusions
In ART-naïve PLWH, high-dose antioxidant, micronutrient supplementation compared to RDA supplementation had no significant effect on disease progression or ART initiation.

Clinical trial registration
ClinicalTrials.gov Identifier: NCT00798772.
cacna2d3, a voltage-gated calcium channel subunit, functions in vertebrate habituation learning and the startle sensitivity threshold
Authors: Santistevan Nicholas J. , Nelson Jessica C. , Ortiz Elelbin A. , Miller Andrew H. , Kenj Halabi Dima , Sippl Zoë A. , Granato Michael , Grinblat Yevgenya 
Published: July 2022

Background
The ability to filter sensory information into relevant versus irrelevant stimuli is a fundamental, conserved property of the central nervous system and is accomplished in part through habituation learning. Synaptic plasticity that underlies habituation learning has been described at the cellular level, yet the genetic regulators of this plasticity remain poorly understood, as do circuits that mediate sensory filtering.

Methods
To identify genes critical for plasticity, a forward genetic screen for zebrafish genes that mediate habituation learning was performed, which identified a mutant allele, doryp177, that caused reduced habituation of the acoustic startle response. In this study, we combine whole-genome sequencing with behavioral analyses to characterize and identify the gene affected in doryp177 mutants.

Results
Whole-genome sequencing identified the calcium voltage-gated channel auxiliary subunit alpha-2/delta-3 (cacna2d3) as a candidate gene affected in doryp177 mutants. Behavioral characterization of larvae homozygous for two additional, independently derived mutant alleles of cacna2d3, together with failure of these alleles to complement doryp177, confirmed a critical role for cacna2d3 in habituation learning. Notably, detailed analyses of the acoustic response in mutant larvae also revealed increased startle sensitivity to acoustic stimuli, suggesting a broader role for cacna2d3 in controlling innate response thresholds to acoustic stimuli.

Conclusions
Taken together, our data demonstrate a critical role for cacna2d3 in sensory filtering, a process that is disrupted in human CNS disorders, e.g. ADHD, schizophrenia, and autism.
Alpha globin gene copy number and hypertension risk among Black Americans
Authors: Ruhl A. Parker , Jeffries Neal , Yang Yu , Gutierrez Orlando M. , Muntner Paul , Naik Rakhi P. , Pecker Lydia H. , Mott Bryan T. , Zakai Neil A. , Safford Monika M. , Lange Leslie A. , Winkler Cheryl A. , Irvin Marguerite R. , Cushman Mary , Ackerman Hans C. 
Published: July 2022

Background
Alpha globin is expressed in the endothelial cells of human resistance arteries where it binds to endothelial nitric oxide synthase and limits release of the vasodilator nitric oxide. Genomic deletion of the alpha globin gene (HBA) is common among Black Americans and could lead to increased endothelial nitric oxide signaling and reduced risk of hypertension.

Methods
Community-dwelling US adults aged 45 years or older were enrolled and examined from 2003 to 2007, followed by telephone every 6 months, and reexamined from 2013 to 2016. At both visits, trained personnel performed standardized, in-home blood pressure measurements and pill bottle review. Prevalent hypertension was defined as systolic blood pressure ≥ 140mmHg or diastolic blood pressure ≥ 90mmHg or anti-hypertensive medication use. Droplet digital PCR was used to determine HBA copy number. The associations of HBA copy number with prevalent hypertension, resistant hypertension, and incident hypertension were estimated using multivariable regression.

Results
Among 9,684 Black participants, 7,439 (77%) had hypertension at baseline and 1,044 of those had treatment-resistant hypertension. 1,000 participants were not hypertensive at baseline and participated in a follow up visit; 517 (52%) developed hypertension over median 9.2 years follow-up. Increased HBA copy number was not associated with prevalent hypertension (PR = 1.00; 95%CI 0.98,1.02), resistant hypertension (PR = 0.95; 95%CI 0.86,1.05), or incident hypertension (RR = 0.96; 95%CI 0.86,1.07).

Conclusions
There were no associations between increased HBA copy number and risk of hypertension. These findings suggest that variation in alpha globin gene copy number does not modify the risk of hypertension among Black American adults.
Patent foramen ovale closure: A prospective UK registry linked to hospital episode statistics
Authors: Willits Iain , Keltie Kim , Henderson Robert , de Belder Mark , Linker Nicholas , Patrick Hannah , Powell Helen , Berry Lee , Urwin Samuel , Cole Helen , Sims Andrew J. 
Published: July 2022

Aims
PFO closure is a percutaneous intervention, which aims to reduce risk of recurrent stroke by preventing paradoxical embolism. The objective of this study was to measure procedural safety and longer-term effectiveness of PFO closure in a UK setting.

Methods and results
Prospective registry data from patients with cryptogenic stroke eligible for PFO closure were collected for up to 2 years and linked to routine data sources for additional follow-up. Outcomes of interest included procedural success rate, health related quality of life, and longer-term death and neurological event rates.
A total of 973 PFO closure procedures in 971 patients were included in analysis. Successful device implantation was achieved in 99.4 [95% CI 98.6 to 99.8]% of procedures, with one in-hospital death. During median follow-up of 758 (Q1:Q3 527:968) days, 33 patients experienced a subsequent neurological event, 76% of which were ischaemic in origin. Neurological event rate was 2.7 [95%CI 1.6 to 3.9]% at 1-year (n = 751) and 4.1 [95% CI 2.6 to 5.5]% at 2-years (n = 463) using Kaplan-Meier analysis. Improvements in patient quality of life (utility and visual analogue scale) were observed at 6-weeks and 6-months follow-up.

Conclusion
Our observational study demonstrates that PFO closure for prevention of recurrent stroke is a relatively safe procedure but in routine clinical practice is associated with a slightly higher risk of recurrent neurological events than in randomised trials. We hypothesize that our study enrolled unselected patients with higher baseline risk, who were excluded from randomised trials, but who may benefit from a similar relative reduction in risk from the intervention.
The effect of levocarnitine supplementation on dialysis-related hypotension: A systematic review, meta-analysis, and trial sequential analysis
Authors: Chewcharat Api , Chewcharat Pol , Liu Weitao , Cellini Jacqueline , Phipps Elizabeth A. , Melendez Young Jill A. , Nigwekar Sagar U. 
Published: July 2022

Background
Dialysis patients have been shown to have low serum carnitine due to poor nutrition, deprivation of endogenous synthesis from kidneys, and removal by hemodialysis. Carnitine deficiency leads to impaired cardiac function and dialysis-related hypotension which are associated with increased mortality. Supplementing with levocarnitine among hemodialysis patients may diminish incidence of intradialytic hypotension. Data on this topic, however, lacks consensus.

Methods
We conducted electronic searches in PubMed, Embase and Cochrane Central Register of Controlled Trials from January 1960 to 19th November 2021 to identify randomized controlled studies (RCTs), which examined the effects of oral or intravenous levocarnitine (L-carnitine) on dialysis-related hypotension among hemodialysis patients. The secondary outcome was muscle cramps. Study results were pooled and analyzed utilizing the random-effects model. Trial sequential analysis (TSA) was performed to assess the strength of current evidence.

Results
Eight trials with 224 participants were included in our meta-analysis. Compared to control group, L-carnitine reduced the incidence of dialysis-related hypotension among hemodialysis patients (pooled OR = 0.26, 95% CI [0.10–0.72], p = 0.01, I2 = 76.0%). TSA demonstrated that the evidence was sufficient to conclude the finding. Five studies with 147 participants showed a reduction in the incidence of muscle cramps with L-carnitine group (pooled OR = 0.22, 95% CI [0.06–0.81], p = 0.02, I2 = 74.7%). However, TSA suggested that further high-quality studies were required. Subgroup analysis on the route of supplementation revealed that only oral but not intravenous L-carnitine significantly reduced dialysis-related hypotension. Regarding dose and duration of L-carnitine supplementation, the dose > 4,200 mg/week and duration of at least 12 weeks appeared to prevent dialysis-related hypotension.

Conclusion
Supplementing oral L-carnitine for at least three months above 4,200 mg/week helps prevent dialysis-related hypotension. L-carnitine supplementation may ameliorate muscle cramps. Further well-powered studies are required to conclude this benefit.
Processing incomplete questionnaire data into continuous digital biomarkers for addiction monitoring
Authors: Zetterström Andreas , Dahlberg Gunnar , Lundqvist Sara , Hämäläinen Markku D. , Winkvist Maria , Nyberg Fred , Andersson Karl 
Published: July 2022

Purpose
eHealth systems allow efficient daily smartphone-based collection of self-reported data on mood, wellbeing, routines, and motivation; however, missing data is frequent. Within addictive disorders, missing data may reflect lack of motivation to stay sober. We hypothesize that qualitative questionnaire data contains valuable information, which after proper handling of missing data becomes more useful for practitioners.

Methods
Anonymized data from daily questionnaires containing 11 questions was collected with an eHealth system for 751 patients with alcohol use disorder (AUD). Two digital continuous biomarkers were composed from 9 wellbeing questions (WeBe-i) and from two questions representing motivation/self-confidence to remain sober (MotSC-i). To investigate possible loss of information in the process of composing the digital biomarkers, performance of neural networks to predict exacerbation events (relapse) in alcohol use disorder was compared.

Results
Long short-term memory (LSTM) neural networks predicted a coming exacerbation event 1–3 days (AUC 0.68–0.70) and 5–7 days (AUC 0.65–0.68) in advance on unseen patients. The predictive capability of digital biomarkers and raw questionnaire data was equal, indicating no loss of information. The transformation into digital biomarkers enable a continuous graphical display of each patient’s clinical course and a combined interpretation of qualitative and quantitative aspects of recovery on a time scale.

Conclusion
By transforming questionnaire data with large proportion of missing data into continuous digital biomarkers, the information captured by questionnaires can be more easily used in clinical practice. Information, assessed by the capability to predict exacerbation events of AUD, is preserved when processing raw questionnaire data into digital biomarkers.
PREOPERATIVE CARDIAC REHABILITATION IN A 28 YEARS-OLD WOMAN WITH VALVULAR HEART DISEASE
Authors: Barata Sri Hastuti , Setiawati Erna , Ambarwati Endang 
Published: July 2022

Background: Valvular heart disease (VHD) is one third of all heart disease. In industrialized countries, the prevalence is estimated at 2,5% and in developing countries is between twenty to thirty cases per-1000 people. VHD presents a huge health burden worldwide. If heart failure has occured, surgery is the treatment of choice. Cardiorespiratory fitness declined in post-surgical patients. Preoperative cardiac rehabilitation is considered to be able to increase the cardiorespiratory fitness baseline so that postoperative outcomes are better.Case Presentation: We reported 28 years-old female with severe MS and MR as well as mild TR with symptoms dyspnea on effort which improves with rest. She was the candidate for mitral valve replacement. She was given a 14-days preoperative cardiac rehabilitation program including breathing exercise by pursed lips breathing, effective cough exercise, range of motion exercise, ankle pumping exercise, endurance exercise based on the result of the 6 minutes walking test (6MWT) and inspiratory muscle training (IMT) using threshold IMT. Conclusion: Preoperative cardiac rehabilitation program improved pulmonary functional capacity, cardiorespiratory fitness and functional activity.Keyword: valvular heart disease, cardiac rehabilitation, pulmonary functional capacity, cardiorespiratory fitness.
Mediated recognition in campaigns for justice: The case of the Magdalene laundry survivors
Authors: Vatnøy Eirik , Wheatley Dawn 
Published: July 2022

AbstractThe recognition perspective is a valuable lens through which identity struggles and historical marginalization and abuses can be explored. This study analyzes Ireland’s Justice for Magdalene (JFM) campaign between 2009–2013; JFM was a group that fought for a state apology and redress for women and girls confined to Catholic-run laundries between the 1920s and 1990s. Such institutions formed part of the post-colonial Irish identity and church-state structure, within which many women and girls once suffered. We document the rhetorical dimension of how a resolution was ultimately reached following the campaign’s transformation of women’s individual experiences into the collective voice of an advocacy group with targeted political goals. Focusing on JFM’s public press releases, we consider the media logic and rhetorical strategies of the group, which effectively built a case against the state. At the core of the discussion is the balance between the instrumental and constitutive functions of the organization’s messaging.
Comprehensive physical and chemical characterization highlights the uniqueness of enzymatic gelatin in terms of surface properties
Authors: Zhang Weijie , Zhang Bing , Wang Ying , Lu Weipeng , Wang Jianing , Wang Yihu , Guo Yanchuan 
Published: July 2022

Abstract
To shorten the long process of conventional alkaline extraction of gelatin, an enzyme-aided method was demonstrated, which was simpler, more effective, and environmental friendly. The main properties of enzymatic gelatin and conventional alkaline gelatin were comprehensively analyzed, including rheological properties, foaming properties, emulsifying properties, water absorption capacity, and thermal stability. It was found that enzymatic gelatin exhibits neutral isoelectric points of 7.4–7.8, higher imino acid content (21.85%, on average), and excellent emulsifying properties, thermal stability, and foaming properties (181%, on average), but lower water absorption properties (5.8 g‧g−1, on average). These findings would be beneficial for the future applications of enzymatic gelatin.
Blueprinting morpho-anatomical episodes via green silver nanoparticles foliation
Authors: Hasan Murtaza , Sajjad Muhammad , Zafar Ayesha , Hussain Riaz , Anjum Syed Ishtiaq , Zia Muhammad , Ihsan Zahid , Shu Xugang 
Published: July 2022

Abstract
Traditional fertilization not only enhances the acidification of soil but also leads toward eutrophication. Here stimulatory and inhibitory effects of foliar fertilization of Cordia based silver nanoparticles (AgNPs) were studied on lettuce (Lactuca sativa L.) biomass accumulation, antioxidant activity, and morphological and anatomical modifications. The AgNPs were foliar supplied with a concentration of 25, 50, and 100 ppm along with control (deionized water) and negative control AgNO3 for consecutive 25 days. The L. sativa fresh and dry biomass accumulation were significantly higher by 53%, leaf area by 25%, and leaf water holding capacity by 207% for foliar sprayed at 25 ppm concentration of AgNPs. The application of AgNPs and AgNO3 had significantly shortened the shoot length (52%), while foliar spray of AgNPs promoted the root length (40%). Among different concentrations of AgNPs, the 50 ppm improved the thickness of stem epidermis (44%), hypodermis (130%), and cortex cell area (372%). For antioxidant studies, the 25 ppm of AgNPs depicted the highest anti-oxidative activity for 2,2-diphenyl-1-picryl hydrazyl radical scavenging activity (75%), total antioxidant capacity (167%), total phenolic content (292%), and total reducing power (60%), while 50 ppm showed the maximum activity for total flavonoid content (243%) as compared to control. Thus, we may conclude that the AgNPs have both stimulatory and inhibitory effects on L. sativa. These effects were dependent on the concentration of the nanoparticles and have varied for different growth, antioxidants, and anatomical traits of the plant.