bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating network pharmacology and experimental verification to explore the mechanism of puerarin against oliguria in acute alcoholism
Authors: Wan Mei-Xuan , Huang Xian-Jun , Li Xue , Suan Juan , Xu Li 
Published: October 2022

Purpose: This study was designed to evaluate the pharmacological mechanisms of puerarin against oliguria in acute alcoholism via network pharmacology analysis combined with experimental verification.Methods: First, this study established an acute alcoholism rat model, compared the changes in urine volume in each group, and observed the therapeutic effect of puerarin by H&amp;E staining, biochemical, RT-qPCR, and immunohistochemical analyses. Second, puerarin-related targets were searched in TCMS, PubChem, CNKI, Wanfang, PubMed, and GeenMedical Academic databases. Also, potential disease targets were obtained from the GeneCards, MalaCards, and NCBI-gene databases and genes with puerarin target gene intersections were screened out. The interaction network for co-predicted targets was obtained using the STRING database, and the core targets were imported into Cytoscape for visualization using DAVID Bioinformatics Resources 6.8. The essential genes were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway enrichment analyses to predict related biological processes and significant signaling pathways. Finally, molecular docking was used to examine the interaction of puerarin with key targets, and the core targets were validated further by RT-qPCR and Western blotting.Results: Compared to the model group, the urine volume of the rats was significantly increased after puerarin treatment, and the levels of anti-diuretic hormone (ADH) and aquaporin 2 (AQP2) expression were decreased. Searching the intersection of puerarin and acute alcoholism targets yielded 214 potential targets, 837 biological processes, and 185 signaling pathways involved. The molecular docking results indicated a good affinity between puerarin and key targets (cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cAMP-response element-binding protein (CREB), and c-Fos). RT-qPCR and Western blotting further verified that puerarin could down-regulate the expression of cAMP/PKA/CREB/c-Fos.Conclusion: This study identified the potential targets of puerarin against oliguria in rats with acute alcoholism using network pharmacology and animal experiments. The mechanism may be closely related to the cAMP signaling pathway.
Administration of recombinant human thrombopoietin is associated with alleviated thrombocytopenia in adult intensive care unit patients with pneumonia: A single-center retrospective study
Authors: Chen Bailiang , Xuan Jiabin , Wu Feng , Shi Nengxian , Dai Jianwei , Cai Shumin , An Shengli , Huang Qiaobing , Huang Xiaoling , Chen Zhongqing , Zeng Zhenhua 
Published: October 2022

Background: Recombinant human thrombopoietin (rhTPO) is reported to stimulate platelet production and increase peripheral platelet counts; it is primarily used to manage chemotherapy-induced thrombocytopenia and idiopathic thrombocytopenic purpura. However, the effect of rhTPO in patients with pneumonia and thrombocytopenia remains uncertain.Objective: To assess the association of rhTPO and platelet counts in ICU patients with pneumonia and thrombocytopenia.Materials and Methods: A retrospective cohort study was performed in the ICU department, Nanfang Hospital, Southern Medical University, Guangzhou, China. From January 2016 to April 2021, patients with pneumonia and thrombocytopenia were allocated to two groups—the rhTPO and no-rhTPO groups—according to whether they received rhTPO treatment or not during their ICU stay. Demographical and clinical data were collected and analyzed using statistical software; p &lt; 0.05 was considered statistically significant.Results: Out of 327 patients, 149 were in the rhTPO group and 178 were in the no-rhTPO group. Within the first 7 days, platelet counts increased more for patients in the rhTPO group compared with those in the no-rhTPO group (99.21 ± 102.613 vs. 2.08 ± 43.877, p = 0.000). The clinical recovery rate of platelets increased within 7 days (65.8 vs. 18.5%, p = 0.000) and, after 7 days of enrollment, hemorrhagic scores decreased more apparently in the rhTPO group (2.81 ± 2.856 vs. 1.16 ± 2.123, p = 0.000). Further, bleeding events ceased in 66.7% of the patients in the rhTPO group compared with 37.3% of the patients in the no-rhTPO group (p = 0.000). Less red-blood-cells transfusions were needed in the rhTPO group (3.639 ± 4.630 vs. 5.818 ± 6.858, p = 0.009). Furthermore, through logistic regression, rhTPO administration was found to be an independent indicator that affected the platelet recovery rate within 7 days.Conclusion: This study finds that rhTPO administration is associated with increased platelet counts, alleviated bleeding, and reduced blood transfusion. For patients with pneumonia and thrombocytopenia, rhTPO may be an effective therapeutic drug; however, more RCT trails are needed to confirm our observation.
A chinese medicine formula (kunbixiao granule) for female rheumatoid arthritis: Study protocol for a double-blind, randomized, placebo-controlled trial
Authors: Wan Yingying , Yang Jiaxi , Ma Tianyue , Wang Wenqian , Wang Haonan , Sun Wenting , Ye Wanting , Yang Lin , Kou Qiuai 
Published: October 2022

Introduction: Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting females more than males. Clinical symptoms, disease activity and comorbidities are more severe in females. Moreover, the choice of treatment for females is limited during childbearing age due to the side effects of current drugs. Therefore, developing novel and safer drugs for females is urgently needed. Kunbixiao granules (KBXG), a Chinese medicine formula, has been applied to treat female RA patients in our center as a complementary therapy. However, there is insufficient evidence for its effect. Therefore, we aim to conduct a randomized, controlled, double-blind clinical trial to confirm the efficacy and safety of KBXG for the treatment of female RA.Methods: This study is a single-center, double-blind, randomized, parallel group, placebo-controlled clinical trial. A total of 90 female RA patients with Disease Activity Score for 28 joints (DAS28) &gt; 3.2 will be enrolled. They will be randomly assigned to receive either KBXG or placebo for 12 weeks. The change in DAS28 based on C-reactive protein (DAS28-CRP) and the Clinical Disease Activity Index (CDAI) are the primary outcomes. The secondary outcomes include a rate of achieving 20%, 50% and 70% improvement in the American College Rheumatology criteria (ACR20, ACR50, ACR70), TCM syndrome score, visual analogue scale (VAS), average hands grip strength, the consumption of concomitant medication, Hospital Anxiety and Depression Scale (HADS), lumbar spine bone mineral density (L-BMD) and 7-joint ultrasound score (US7). Any adverse events will also be recorded.Discussion: This trial will provide evidence of KBXG in reducing disease activity, and improving clinical symptoms and quality of life of female RA patients. The long-term effects of KBXG on female RA patients still needs a further follow-up.
Effect of 3 min whole-body and lower limb cold water immersion on subsequent performance of agility, sprint, and intermittent endurance exercise
Authors: Zhang Wei , Ren Shoupeng , Zheng Xinyan 
Published: October 2022

The aim of this study was to investigate the effects of whole-body cold-water immersion (WCWI) and lower-limb cold-water immersion (LCWI) employed during a 15-min recovery period on the subsequent exercise performance as well as to determine the physiological and perceptual parameters in the heat (39°C). Eleven males performed team-sports-specific tests outdoors. The exercise program consisted of two identical exercise protocols (1 and 2) separated by a 15-min recovery period. The participants completed the same tests in each exercise protocol, in the following order: agility t test (t-test), 20-m sprint test (20M-ST), and Yo-Yo Intermittent Endurance Test Level 1 (Yo-Yo). During the recovery period, a 3-min recovery intervention of a passively seated rest (control, CON), WCWI, or LCWI was performed. The t-test and 20M-ST for the CON group were significantly longer during exercise protocol 2, but they were not significantly different between the two exercise protocols for the WCWI and LCWI groups. The completed Yo-Yo distance for the CON and LCWI groups was shorter during exercise protocol 2, but it was not significantly different between the two exercise protocols for the WCWI group. The chest temperature (Tchest), upper arm temperature (Tarm), thigh temperature (Tthigh), mean skin temperature (Tskin), and thermal sensation (TS) values were lower for the WCWI group than for the CON group; but only the Tthigh, Tskin, and TS values were lower for the LCWI group compared to the CON group. The Tchest, Tarm, Tskin, and TS values after the intervention were lower for the WCWI group than for the LCWI group. None of the three intervention conditions affected the core temperature (Tcore), heart rate (HR), or rating of perceived exertion (RPE). These results suggest that WCWI at 15°C for 3 min during the 15-min recovery period attenuates the impairment of agility, sprint, and intermittent-endurance performance during exercise protocol 2, but LCWI only ameliorates the reduction of agility and sprint performance. Furthermore, the ergogenic effects of WCWI and LCWI in the heat are due, at least in part, to a decrease of the Tskin and improvement of perceived strain.
Optimization and validation of blade parameters for inter-row weeding wheel in paddy fields
Authors: Zhang Yongzheng , Tian Liang , Cao Chengmao , Zhu Chengliang , Qin Kuan , Ge Jun 
Published: October 2022

The performance of existing rice-paddy weeding machines is not optimal. In this study, the influence of the installation angle of the weeding-wheel blade on cutting resistance and soil-slippage ability was analyzed. The optimal blade angle of the weeding wheel (i.e., the angle at which the resistance to the weeding wheel is minimal and the disturbance speed of the soil maximal) was shown to be&lt; 20°; numerical simulation showed the actual optimal value to be 0°. Different weeding depths (30, 40, and 50 mm), rotation speed of weeding wheel (120, 180, and 240 r/min), and weeder forward speeds (0.3, 0.6, and 0.9 m/s) were used as test factors, and the rates of seedling injury and weeding were used as performance-evaluation criteria to optimize the machine in a secondary orthogonal-rotation combination test. Field experiments showed that the weeding wheel can exhibit optimal working performance under the operating conditions of weeding depth of 39 mm, rotation speed of 175 r/min, and forward speed of 0.6 m/s. The seedling injury and weeding rates were 4.4% and 88.2%, respectively, which were consistent with the numerically predicted results and met the agronomic requirements. This study provides a technical reference for the improvement of paddy-field weeding components.
Study on a Bayes evaluation of the working ability of petroleum workers in the Karamay region, Xinjiang, China
Authors: An Hengqing , Xu Lei , Liu Yuanyuan , Ma Dongsheng , Zhang Dajun , Tao Ning 
Published: October 2022

ObjectivesUse Bayes statistical methods to analyze the factors related to the working ability of petroleum workers in China and establish a predictive model for prediction so as to provide a reference for improving the working ability of petroleum workers.Materials and methodsThe data come from the health questionnaire database of petroleum workers in the Karamay region, Xinjiang, China. The database contains the results of a health questionnaire survey conducted with 4,259 petroleum workers. We established an unsupervised Bayesian network, using Node-Force to analyze the dependencies between influencing factors, and established a supervised Bayesian network, using mutual information analysis methods (MI) to influence factors of oil workers’ work ability. We used the Bayesian target interpretation tree model to observe changes in the probability distribution of work ability classification under different conditions of important influencing factors. In addition, we established the Tree Augmented Naïve Bayes (TAN) prediction model to improve work ability, make predictions, and conduct an evaluation.Results(1) The unsupervised Bayesian network shows that there is a direct relationship between shoulder and neck musculoskeletal diseases, anxiety, working age, and work ability, (2) The supervised Bayesian network shows that anxiety, depression, shoulder and neck musculoskeletal diseases (Musculoskeletal Disorders, MSDs), low back musculoskeletal disorders (Musculoskeletal Disorders, MSDs), working years, age, occupational stress, and hypertension are relatively important factors that affect work ability. Other factors have a relative impact on work ability but are less important.ConclusionAnxiety, depression, shoulder and neck MSDs, waist and back MSDs, and length of service are important influencing factors of work ability. The Tree Augmented Naïve Bayes prediction model has general performance in predicting workers’ work ability, and the Bayesian model needs to be deepened in subsequent research and a more appropriate forecasting method should be chosen.
The changes in psychological symptoms of COVID-19 patients after “re-positive”
Authors: Wang Xing , Fan Qinyi , Li Yunyue , Xiao Junjian , Huang Yanyan , Guo Tiantian , Chen Hongguang , Li Mengqian 
Published: October 2022

BackgroundPrevious studies have showed that individuals infected with COVID-19 were more likely to report psychological symptoms. However, little is known about the changes from testing positive to negative to positive again.MethodsThis survey was conducted through the questionnaires including the 7-item Generalized Anxiety Disorder (GAD-7), the 9-item Patient Health Questionnaire (PHQ-9), as well as the Self-Rating Scale of Sleep (SRSS) to explore the psychological status of COVID-19 and re-positive cases.″re-positive″ is defined as a positive RT-PCR test at any time during the recovery period after testing negative.ResultsA total of 94 COVID-19 patients presented the prevalence rates of anxiety, depression, insomnia, and any of the three psychological symptoms being 26.6, 8.6, 12.8, and 31.9%, respectively. Among these, 32 cases were re-tested positive during the recovery period, with the prevalence rates of anxiety, depression, insomnia, and any of the three psychological symptoms being 21.9, 18.7, 31.2, and 37.5%, respectively. The psychological status after re-positive showed a significant decrease in anxiety (P = 0.023), an increase in depression, and a significant rise in insomnia (P = 0.035). For those with no psychological symptoms during initial-positive, after re-positive, 5.88% reported anxiety, 5.88% reported depression, and 11.76% reported insomnia. For those who experienced only anxiety symptoms during initial-positive, after re-positive, 33.3% reported depression, and 33.3% reported insomnia.ConclusionsOur findings encompassed the urgent concern for anxiety in initial-positive COVID-19 patients, depression in re-positive COVID-19 patients, and insomnia in both initial and re-positive patients, hence enabling targeted interventions for appeasing the psychological burden of COVID-19 patients.
Association of elevated glycated hemoglobin and obesity with afternoon napping for more than 1 h in young and middle-aged healthy adults
Authors: Al-Abri Mohammed A. , Al Lawati Ibtisam , Al Zadjali Fahad 
Published: October 2022

IntroductionSleep has different patterns followed worldwide and can be influenced by social, cultural, and environmental factors. Daytime napping is commonly practiced in different parts of the world with controversial results of its effect on glucose metabolism. The current study aims to examine the association of afternoon napping and night sleep duration with metabolic derangements.MethodsThis is a cross-sectional study involving young adults and middle-aged subjects. Anthropometric measurements were taken for height and weight and hip and waist ratio. Consented subjects were asked to wear actigraphy for 1 week and run their usual daily activities. Home sleep apnea testing was performed to exclude obstructive sleep apnea. Subjects had been asked to come fasting on day seven for blood collection to test for fasting glucose, glycated hemoglobin, lipid profile, and insulin.ResultsA total of 405 subjects were involved to complete the study (52% male, 48% female). The mean age of participants was 32.8 ± 11.5 years. The study indicated that the duration of afternoon napping was significantly associated with abnormal glycated hemoglobin (HbA1c &gt; 5.7%) (p = 0.01) and body mass index (p = 0.046) independent of age, gender, and nocturnal sleep duration. Nocturnal sleep duration was associated with increased insulin level (p = 0.04).ConclusionAfternoon napping is associated with an increased level of glycated hemoglobin and obesity and that may predispose to the development of type 2 diabetes mellitus.
Factors influencing the efficiency of cellphone-based CBT for treating sleep disorders
Authors: Hu Nannan , Xu You , Mao Hongjing 
Published: October 2022

ObjectiveThis survey aimed to better comprehend the factors influencing patient response to insomnia treatment.MethodsWe conducted an online survey. A total of 1,395 patients completed the questionnaire at baseline. Insomnia, anxiety and depressive symptoms were evaluated using the Pittsburgh Sleep Quality Index (PSQI), 7-item Generalized Anxiety Disorder assessment (GAD-7) and 9-item Patient Health Questionnaire (PHQ-9), respectively. A total of 488 patients completed at least two surveys (baseline and monthly surveys thereafter) and reported that the online CBT was effective at the 1-year follow-up. The 488 patients were divided into three groups: the rapid (treatment effective at 4 weeks), intermediate (4–16 weeks), and delayed-response group (over 16 weeks).ResultsAnalysis of the demographic characteristics of the 488 patients did not reveal significant sex differences among the three groups (P = 0.111). However, the groups significantly differed in age (P = 0.001) and education (P = 0.006). Compared to the rapid response group, the delayed-response group had a higher mean age (P &lt; 0.01) and a slightly lower level of education. The duration of the disorder was longer in the delayed-response group. Multivariate logistic regression showed that male sex, junior high school education, and higher PSQI were independent risk factors for the delayed response to treatment.ConclusionMany factors affected the efficiency of insomnia treatment. Male sex, junior school education, and a high PSQI score predicted delayed response to insomnia treatment.
Parental smoking exposure before and during pregnancy and offspring attention-deficit/hyperactivity disorder risk: A Chinese child and adolescent cohort study
Authors: Liu Dong , Ren Yaping , Wu Tianfeng , Shen Huiping , Yan Peijing , Meng Yu , Zhang Qianlong , Zhang Jun , Bai Pinqing , Zhao Jian 
Published: October 2022

BackgroundPrevious studies revealed that maternal smoking exposure during pregnancy was an essential risk factor for offspring developing attention-deficit/hyperactivity disorder (ADHD). The impact of paternal smoking exposure 1 year before pregnancy on offspring ADHD risk is still unclear.MethodsThe present study included 2,477 school-age children and their parents from the Shanghai Child and Adolescent Health Cohort who had complete data for offspring ADHD diagnosis and parents' smoking exposure before and during pregnancy information. A multivariate logistic regression model and Firth's logistic regression model were used to determine the associations of paternal smoking and parental smoke exposure patterns before and during pregnancy with offspring ADHD risk.ResultsChildren whose fathers smoked before pregnancy had a higher risk of developing ADHD [odds ratio (OR) = 2.59, 95% confidence interval (CI): 1.35–4.98] compared to those whose fathers had never been exposed to smoking. Similarly, parents who were exposed to smoking or second-hand smoke before pregnancy had 1.96 times (OR = 1.96, 95% CI: 1.19–3.22) more likely to have offspring with ADHD. Moreover, children whose parents were exposed to smoking both before and during pregnancy were 2.01 times (OR = 2.01, 95% CI: 1.29–3.12) more likely to develop ADHD.ConclusionPaternal smoking before pregnancy and parental smoking exposure 1 year ahead of and throughout pregnancy were all risk factors for offspring developing ADHD.
Screen time and myopia: A serial multiple mediator SEM analysis
Authors: Xie Jinchen , Lu Chuntian , Zhu Jie 
Published: October 2022

BackgroundCOVID-19 has influenced education systems worldwide, and significantly increased screen time for college students, posing a potential risk of myopia. In China, ninety percent of college students suffer from myopia. Excessive screen time changes college students' lifestyles, imposes potential health risks, and affects opportunities for employment. It is important to identify the potential correlation between screen time use and myopia among college students.MethodsThis paper conducted a nationwide experiment using Chinese college students and set a multiple-mediator SEM model to analyze the potential correlation between screen time and myopia. The two mediators were sedentary behavior and physical activity, respectively.ResultsWe obtained three valuable conclusions as follows: First, there was no significant direct relationship between screen time and myopia among Chinese college students during the COVID-19 pandemic. Second, sedentary behavior and physical activity significantly predicted the increase/decrease of myopia among Chinese college students, respectively. Third, a serial multiple mediator that encompassed sedentary behavior and physical activity sequentially fully mediated the relationship between screen time and myopia.ConclusionsAlthough there was no directly significant relationship between screen time and myopia, screen time can indirectly influence the risk of suffering myopia by influencing sedentary behavior and physical activity. Our study demonstrates the need to prevent the potential influence of overuse of electronic devices on myopia in college students, especially during the COVID-19 pandemic.