bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0011292 knockdown mitigates progression and drug resistance in PTX‐resistant non‐small‐cell lung cancer cells by regulating miR‐433‐3p/CHEK1 axis
Authors: Jin Ming , Zhang Fengping , Li Qiubo , Xu Ruiqi , Liu Ying , Zhang Yong 
Published: March 2022

AbstractBackgroundNon‐small‐cell lung cancer (NSCLC) is one of the most common malignant tumors on earth. Circular RNAs have been disclosed to be vital regulators in the chemoresistance and development of diverse cancers, including NSCLC. Here, we attempted to explore the function of circ_0011292 in paclitaxel (PTX)‐resistant NSCLC cells.MethodsQuantitative real‐time polymerase chain reaction or western blot was performed to detect the expression of circ_0011292, microRNA‐433‐3p (miR‐433‐3p), and checkpoint kinase 1 (CHEK1). Ribonuclease R (RNase R) assay was performed to assess the stability of circ_0011292. Cell Counting Kit‐8 assay was conducted to evaluate the half maximal inhibitory concentration of PTX and cell viability. Cell proliferation was monitored by Edu incorporation and colony formation assays. Cell cycle and apoptosis were detected by flow cytometry. Transwell assay was implemented to assess cell migration and invasion. Western blot assay was utilized to determine the protein levels. Dual‐luciferase reporter assay was carried out to verify the targeted interaction between miR‐433‐3p and circ_0011292 or CHEK1. Xenograft tumor model was constructed for determining the effect of circ_0011292 in NSCLC growth in vivo.ResultsCirc_0011292 was upregulated in PTX‐resistant NSCLC tissues and cells. Circ_0011292 or CHEK1 knockdown enhanced PTX sensitivity and cell apoptosis, and repressed cell proliferation, migration, and invasion in PTX‐resistant NSCLC cells. Mechanistically, circ_0011292 was a sponge of miR‐433‐3p and miR‐433‐3p directly targeted CHEK1. Meanwhile, silencing miR‐433‐3p or overexpressing CHEK1 respectively abrogated the impacts of circ_0011292 deletion or miR‐433‐3p introduction on PTX resistance and cell progression in PTX‐resistant NSCLC cells in vitro. Moreover, circ_0011292 could positively modulate CHEK1 expression through sponging miR‐433‐3p. In addition, circ_0011292 knockdown retarded tumor growth of NSCLC in vivo.ConclusionCirc_0011292 could accelerate PTX resistance and cell malignant progression of NSCLC cells partially through the regulation of circ_0011292/miR‐433‐3p/CHEK1 axis.
Rare ashy dermatosis‐like hyperpigmentation associated with osimertinib
Authors: Funai Kazuhito 
Published: March 2022

AbstractOsimertinib, a third‐generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), is the most reliable EGFR‐TKI and is widely used as the first‐line treatment for EGFR mutation‐positive non‐small cell lung cancer. Pigmentary changes caused by EGFR‐TKIs are unusual, and to the best of my knowledge, hyperpigmentation with osimertinib has rarely been reported as a skin‐related adverse event. Here, I report a case of osimertinib‐associated ashy dermatosis‐like hyperpigmentation on imaging. Although reducing the dose of osimertinib to 40 mg did not improve pigmentation, osimertinib use was continued due to its clinical and radiological benefit, which persisted for a long time.
Glial cell line‐derived neurotrophic factor contributes to alcoholic‐induced liver injury by regulating the NF‐κB pathway
Authors: Liu Xuling , Yang Guangyue , Sun Tiantian , Tao Le , Shen Dongxiao , Zhang Wei , Zhang Jie , Xue Dongying , Chen Bei , Wu Liu , Liu Cheng , Ma Wenting 
Published: March 2022

AbstractBackgroundAlcoholic liver disease (ALD) is associated with high morbidity and mortality worldwide. The pathogenesis of ALD is not completely understood. Although accumulating evidence suggests an important role of glial cell line‐derived neurotrophic factor (GDNF) in several diseases, there are no data concerning its role in ALD. This study compared patients with ALD with control subjects and used a mouse model and a cell culture model to investigate the function of GDNF in ALD and its mechanism of action in hepatocyte injury.MethodsSerum levels of GDNF were measured in 25 patients with ALD and 25 healthy control subjects. A 4‐week Lieber–DeCarli ethanol (EtOH) liquid diet combined with the Gao–Binge model was used in the mouse study. Mouse primary hepatocytes and Huh‐7 cells were used for cell experiments. The parameters of liver injury, inflammatory cytokines, and lipid metabolism were measured.ResultsPatients with alcoholic hepatitis had higher serum GDNF than control subjects. Expression of GDNF mRNA and protein was markedly increased in mice in the chronic‐plus‐binge ALD mouse model. The level of GDNF mRNA was upregulated in primary hepatic stellate cells isolated from ethanol‐fed mouse liver. Ethanol induced GDNF expression in LX2 cells. The levels of inflammatory cytokines (tumor necrosis factor α, interleukin 1β, and monocyte chemotactic protein 1) were significantly increased after GDNF stimulation in primary hepatocytes and Huh‐7 cells. After GDNF stimulation, levels of both p‐AKT and p‐NF‐κB were significantly increased in primary hepatocytes and Huh‐7 cells. The NF‐κB activity induced by GDNF was significantly decreased by an NF‐κB inhibitor, which limited hepatocyte injury and inflammation.ConclusionsThe concentration of GDNF is increased in the circulation of ALD patients. GDNF promotes alcohol‐induced liver injury and inflammation via the activation of NF‐κB, which mediates hepatocyte injury and inflammatory cytokine expression. Based on these findings, GDNF is a potential therapeutic target for preventing or ameliorating liver injury in ALD.
Estimating global determinants of leopard home range size in a changing world
Authors: Rodríguez‐Recio M , Burgos T , Krofel M , Lozano J , Moleón M , Virgós E 
Published: March 2022

AbstractMovement is a fundamental process in animal ecology. For many species, such as large carnivores, movement patterns are greatly shaped by a combination of ecological and anthropogenic factors. Understanding how these factors impact the roaming capacity of large carnivores is essential to forecast risks and design long‐term conservation strategies. The leopard (Panthera pardus) is a generalist predator broadly distributed over varied and different environments, but global leopard populations are declining at a concerning rate and conservation actions are pressing. This scenario makes the leopard a suitable species to understand how global ecological and anthropogenic drivers affect the spatial behaviour of large carnivores and how these should inform conservation efforts. We compiled data from local studies worldwide and used macroecological (climatic, productivity, and human footprint), and intra‐ and interspecific (conspecifics, competitors and prey) predictors to model the roaming requirements of leopards based on home range sizes. Male home range size was largely and positively related to the range sizes of local females and inversely to vegetation productivity. For females, higher seasonal variations in temperature like the observed in arid areas were associated with larger home ranges, while increased human impact resulted in smaller home ranges likely due to concentrated food resources such as domestic species. These predictors are linked to threatening global change processes due to anthropogenic activities that will likely impact the roaming behaviour of leopards in the coming decades with potential consequences for their populations worldwide. Our results provide crucial information towards the development of integrative research linking macroecological and local variables with global change predictions that can inform conservation programmes addressing future risks of degradation, endangerment and human‐leopard conservation conflicts.
Progesterone modulates CD4+CD25+FoxP3+ regulatory T Cells and TGF‐β1 in the maternal‐fetal interface of the late pregnant mouse
Authors: Yang Qianqian , Li Meihui , Zhao Ming , Lu Feifan , Yu Xiaomin , Li Li , Gu Zhongyi , Deng Yifang , Guan Rui 
Published: March 2022

AbstractObjectiveProgesterone supplementation is recommended to prevent spontaneous preterm birth (sPTB) in clinical practice. However, the exact mechanism is still unclear. This study aims to better understand the mechanisms that progesterone can prevent PTB.MethodsLate pregnant mice were given various doses of progesterone receptor antagonist mifepristone, and pregnancy outcomes were observed. Then, non‐pregnant and pregnant mice were given a subcutaneous injection of 40 mg/kg progesterone and 5 mg/kg mifepristone, respectively. CD4+CD25+FoxP3+ Treg cells in peripheral blood and decidua basalis were detected by FACS. Expressions of FoxP3 and TGF‐β1 in the decidua basalis were detected.ResultsMifepristone induced preterm birth, and an obvious dose‐response was found. Proportions of CD4+CD25+FoxP3+ Treg cells in the peripheral blood of non‐pregnant mice increased significantly after progesterone injection. CD4+CD25+FoxP3+ Treg cells in the peripheral blood of pregnant mice increased significantly compared with those of non‐pregnant mice. In pregnant mice, mifepristone significantly decreased the proportions of CD4+CD25+FoxP3+ Treg cells in peripheral blood, and reduced proportions of Treg cells at the maternal‐fetal interface and expressions of FoxP3 and TGF‐β1 in the maternal‐fetal interface. Total 40 mg/kg of progesterone did not increase CD4+CD25+FoxP3+ Treg in the peripheral blood of pregnant mice, but increased proportions of Treg cells at the maternal‐fetal interface and up‐regulated FoxP3 and TGF‐β1 expressions in the maternal‐fetal interface.ConclusionProgesterone promotes pregnancy immune homeostasis by up‐regulating Treg cells and TGF‐β1 expression in the maternal‐fetal interface. It may be one of the mechanisms of progesterone in preventing sPTB.
Battles forSocio‐SpatialHegemony in the Exilic Space of Exarcheia
Authors: Karlin John Ryan 
Published: March 2022

AbstractThe neighbourhood of Exarcheia in Athens, Greece has long been recognised as the socio‐spatial epicentre of the Athenian anarchist and anti‐authoritarian milieu wherein several self‐organised spaces, groups, and initiatives have thrived. In this paper, I characterise Exarcheia as an exilic space within the capitalist world‐system. Exilic spaces are paradoxical spaces of relative and precarious autonomy grounded in the principles of self‐organisation, solidarity, and mutual aid. While I highlight Exarcheia’s exilic status, I also investigate significant internal problematics and tensions. Specifically, I investigate the construction and implementation of a hegemonic project at the exilic scale revolving around the perception that certain anti‐social actors were gaining ground in the neighbourhood whose behaviours constituted a direct threat to Exarcheia’s exilic status. I then problematise the hegemonic consensus and identify a counter‐hegemonic response consisting of marginalised voices opposed to the hegemonic project in both theory and practice, signalling a crisis of exilic hegemony.
Advances in microfabrication technologies in tissue engineering and regenerative medicine
Authors: Nadine Sara , Chung Ada , Diltemiz Sibel Emir , Yasuda Brooke , Lee Charles , Hosseini Vahid , Karamikamkar Solmaz , de Barros Natan Roberto , Mandal Kalpana , Advani Shailesh , Zamanian Benjamin Behnam , Mecwan Marvin , Zhu Yangzhi , Mofidfar Mohammad , Zare Mohammad Reza , Mano João , Dokmeci Mehmet Remzi , Alambeigi Farshid , Ahadian Samad 
Published: March 2022

AbstractBackgroundTissue engineering provides various strategies to fabricate an appropriate microenvironment to support the repair and regeneration of lost or damaged tissues. In this matter, several technologies have been implemented to construct close‐to‐native three‐dimensional structures at numerous physiological scales, which are essential to confer the functional characteristics of living tissues.MethodsIn this article, we review a variety of microfabrication technologies that are currently utilized for several tissue engineering applications, such as soft lithography, microneedles, templated and self‐assembly of microstructures, microfluidics, fiber spinning, and bioprinting.ResultsThese technologies have considerably helped us to precisely manipulate cells or cellular constructs for the fabrication of biomimetic tissues and organs. Although currently available tissues still lack some crucial functionalities, including vascular networks, innervation, and lymphatic system, microfabrication strategies are being proposed to overcome these issues. Moreover, the microfabrication techniques that have progressed to the preclinical stage are also discussed.ConclusionsThis article aims to highlight the advantages and drawbacks of each technique and areas of further research for a more comprehensive and evolving understanding of microfabrication techniques in terms of tissue engineering and regenerative medicine applications.
Two sides of one medal: Arable weed vegetation of Europe in phytosociological data compared to agronomical weed surveys
Authors: Bürger Jana , Küzmič Filip , Šilc Urban , Jansen Florian , Bergmeier Erwin , Chytrý Milan , Cirujeda Alicia , Fogliatto Silvia , Fried Guillaume , Dostatny Denise F. , Gerowitt Bärbel , Glemnitz Michael , González‐Andújar José L. , Hernández Plaza Eva , Izquierdo Jordi , Kolářová Michaela , Lososová Zdeňka , Metcalfe Helen , Ņečajeva Jevgenija , Petit Sandrine , Pinke Gyula , Rašomavičius Valerijus , von Redwitz Christoph , Schumacher Matthias , Ulber Lena , Vidotto Francesco 
Published: March 2022

AbstractQuestionsTwo scientific disciplines, vegetation science and weed science, study arable weed vegetation, which has seen a strong diversity decrease in Europe over the last decades. We compared two collections of plot‐based vegetation records originating from these two disciplines. The aim was to check the suitability of the collections for joint analysis and for addressing research questions from the opposing domains. We asked: are these collections complementary? If so, how can they be used for joint analysis?LocationEurope.MethodsWe compared 13 311 phytosociological relevés and 13 328 records from weed science, concerning both data collection properties and the recorded species richness. To deal with bias in the data, we also analysed different subsets (i.e., crops, geographical regions, organic vs conventional fields, center vs edge plots).ResultsRecords from vegetation science have an average species number of 19.0 ± 10.4. Metadata on survey methodology or agronomic practices are rare in this collection. Records from weed science have an average species number of 8.5 ± 6.4. They are accompanied by extensive methodological information. Vegetation science records and the weed science records taken at field edges or from organic fields have similar species numbers. The collections cover different parts of Europe but the results are consistent in six geographical subsets and the overall data set. The difference in species numbers may be caused by differences in methodology between the disciplines, i.e., plot positioning within fields, plot sizes, or survey timing.ConclusionThis comparison of arable weed data that were originally sampled with a different purpose represents a new effort in connecting research between vegetation scientists and weed scientists. Both collections show different aspects of weed vegetation, which means the joint use of the data is valuable as it can contribute to a more complete picture of weed species diversity in European arable landscapes.
How fairness and dominance guide young children’s bargaining decisions
Authors: Grueneisen Sebastian , Tomasello Michael 
Published: March 2022

AbstractReaching agreements in conflicts is an important developmental challenge. Here, German 5‐year‐olds (N = 284, 49% female, mostly White, mixed socioeconomic backgrounds; data collection: June 2016–November 2017) faced repeated face‐to‐face bargaining problems in which they chose between fair and unfair reward divisions. Across three studies, children mostly settled on fair divisions. However, dominant children tended to benefit more from bargaining outcomes (in Study 1 and 2 but not Study 3) and children mostly failed to use leverage to enforce fairness. Communication analyses revealed that children giving orders to their partner had a bargaining advantage and that children provided and responded to fairness reasons. These findings indicate that fairness concerns and dominance are both key factors that shape young children's bargaining decisions.
Overexpression of GRK6 alleviates chronic visceral hypersensitivity through downregulation of P2Y6 receptors in anterior cingulate cortex of rats with prenatal maternal stress
Authors: Tian Yuan‐Qing , Li Jia‐Hui , Li Yong‐Chang , Xu Yu‐Cheng , Zhang Ping‐An , Wang Qian , Li Rui , Xu Guang‐Yin 
Published: March 2022

AbstractAimsVisceral hypersensitivity is a major clinic symptom in patients with irritable bowel syndrome (IBS). Anterior cingulate cortex (ACC) is involved in processing the information of pain. Both G protein‐coupled receptor kinase 6 (GRK6) and P2Y purinoceptor 6 (P2Y6) are associated with neuroinflammation and pathological pain. The aim of this study was to investigate the interaction between GRK6 and P2Y6 in ACC in the development of visceral hypersensitivity of adult offspring rats with prenatal maternal stress (PMS).MethodsVisceral hypersensitivity was quantified by abdominal withdrawal reflex threshold to colorectal distension (CRD). The expression and cellular distribution of GRK6 and P2Y6 were determined by Western blotting, qPCR, and fluorescence immunohistochemistry. Co‐immunoprecipitation was used to evaluate the interaction between GRK6 and P2Y6.ResultsThe mRNA and protein levels of GRK6 were significantly decreased in ACC of PMS rats. The injection of GRK6 overexpression virus significantly attenuated visceral hypersensitivity of PMS rats. P2Y6’s mRNA level, protein level, and ratio of membrane protein over total protein expression was markedly increased in PMS rats. P2Y6 antagonist MRS2578 microinjection reversed visceral hypersensitivity of PMS rats. GRK6 overexpression significantly reduced P2Y6’s expression in membrane proteins and P2Y6’s ratio of membrane protein over total protein expression.ConclusionsThese results indicate that decreased GRK6 leads to the accumulation of P2Y6 at neuron membrane in ACC, thereby contributing to visceral hypersensitivity of PMS rats.
Dying for love: Illegal international trade in hummingbird love charms
Authors: Trail Pepper W. 
Published: March 2022

AbstractHummingbirds (family Trochilidae) are among the most recognizable groups of birds, due to their tiny size, supremely maneuverable flight, and iridescent plumage. They are essential pollinators in ecosystems throughout the New World, and are welcome visitors to gardens and nectar feeders in every corner of the United States. Despite this familiarity, few people are aware that hummingbirds are the objects of active illegal international trade. The practice of incorporating the bodies of hummingbirds in love charms, known aschuparrosacharms, is a centuries‐old tradition in Mexico. The practice has now spread to the United States. This report summarizes investigations of the hummingbird trade by the U.S. Fish and Wildlife Service from 2013 to 2021. A total of 905 hummingbird carcasses were recovered in these investigations, representing 22 different species, 18 of which are protected by the Migratory Bird Treaty Act. While it appears that all hummingbirds were killed and packaged in Mexico, many originated from migratory U.S. populations. The species most frequently identified inchuparrosaswas Ruby‐throated Hummingbird, which nests entirely in the United States and occurs in Mexico only in the non‐breeding season. Much remains to be learned about the dimensions of thechuparrosatrade, and its possible impacts on hummingbird populations.
Pharmacogenomic landscape of Indian population using whole genomes
Authors: Sahana S. , Bhoyar Rahul C. , Sivadas Ambily , Jain Abhinav , Imran Mohamed , Rophina Mercy , Senthivel Vigneshwar , Kumar Diwakar Mohit , Sharma Disha , Mishra Anushree , Sivasubbu Sridhar , Scaria Vinod 
Published: March 2022

AbstractEthnic differences in pharmacogenomic (PGx) variants have been well documented in literature and could significantly impact variability in response and adverse events to therapeutics. India is a large country with diverse ethnic populations of distinct genetic architecture. India’s national genome sequencing initiative (IndiGen) provides a unique opportunity to explore the landscape of PGx variants using population‐scale whole genome sequences. We have analyzed the IndiGen variation dataset (N = 1029 genomes) along with global population scale databases to map the most prevalent clinically actionable and potentially deleterious PGx variants among Indians. Differential frequencies for the known and novel variants were studied and interaction of the disrupted PGx genes affecting drug responses were analyzed by performing a pathway analysis. We have highlighted significant differences in the allele frequencies of clinically actionable PGx variants in Indians when compared to the global populations. We identified 134 mostly common (allele frequency [AF] > 0.1) potentially deleterious PGx variants that could alter or inhibit the function of 102 pharmacogenes in Indians. We also estimate that on, an average, each Indian individual carried eight PGx variants (single nucleotide variants) that have a direct impact on the choice of treatment or drug dosing. We have also highlighted clinically actionable PGx variants and genes for which preemptive genotyping is most recommended for the Indian population. The study has put forward the most comprehensive PGx landscape of the Indian population from whole genomes that could enable optimized drug selection and genotype‐guided prescriptions for improved therapeutic outcomes and minimizing adverse events.