bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phospholipase D1 promotes astrocytic differentiation through the FAK/AURKA/STAT3 signaling pathway in hippocampal neural stem/progenitor cells
Authors: Kang Min-Jeong , Jin Nuri , Park Shin-Young , Han Joong-Soo 
Published: June 2022

Abstract
Phospholipase D1 (PLD1) plays a crucial role in cell differentiation of different cell types. However, the involvement of PLD1 in astrocytic differentiation remains uncertain. In the present study, we investigate the possible role of PLD1 and its product phosphatidic acid (PA) in astrocytic differentiation of hippocampal neural stem/progenitor cells (NSPCs) from hippocampi of embryonic day 16.5 rat embryos. We showed that overexpression of PLD1 increased the expression level of glial fibrillary acidic protein (GFAP), an astrocyte marker, and the number of GFAP-positive cells. Knockdown of PLD1 by transfection with Pld1 shRNA inhibited astrocytic differentiation. Moreover, PLD1 deletion (Pld1−/−) suppressed the level of GFAP in the mouse hippocampus. These results indicate that PLD1 plays a crucial role in regulating astrocytic differentiation in hippocampal NSPCs. Interestingly, PA itself was sufficient to promote astrocytic differentiation. PA-induced GFAP expression was decreased by inhibition of signal transducer and activation of transcription 3 (STAT3) using siRNA. Furthermore, PA-induced STAT3 activation and astrocytic differentiation were regulated by the focal adhesion kinase (FAK)/aurora kinase A (AURKA) pathway. Taken together, our findings suggest that PLD1 is an important modulator of astrocytic differentiation in hippocampal NSPCs via the FAK/AURKA/STAT3 signaling pathway.
A Machine Learning-based Characterization Framework for Parametric Representation of Nonlinear Sloshing
Authors: Luo Xihaier , Kareem Ahsan , Yu Liting , Yoo Shinjae 
Published: June 2022

Abstract
There is a growing interest in developing a parametric representation of liquid sloshing inside a container stemming from its practical applications in modern engineering systems. The resonant excitation due to sloshing, on the other hand, can cause unstable and nonlinear water waves, resulting in chaotic motions and non-Gaussian wave profiles. This paper presents a novel machine learning-based framework for nonlinear liquid sloshing representation learning. The proposed method is a parametric modeling technique that is based on sequential learning and sparse regularization. The dynamics are categorized into two parts: linear evolution and nonlinear forcing. The former advances the dynamical system in time on an embedded manifold, while the latter causes divergent behaviors in temporal evolution, such as bursting and switching. The effectiveness of the proposed framework is demonstrated using an experimental dataset of liquid sloshing in a tank under horizontal excitation with a wide range of frequencies and vertical slat screen settings immersed in the tank perpendicular to the excitation.
Assessment of important cardiovascular risk factors using ML methods: a randomized controlled trial
Authors: Pella Zuzana , Lohaj Oliver , Pella Dominik , Paralič Ján 
Published: June 2022

Abstract
Background: High prevalence and mortality of cardiovascular diseases (CVD) are global problems. Many countries focus their healthcare on secondary treatment, and primary prevention is in the background. Focusing on risk factors (RF) screening of CVD in personalized medicine has great potential for the future. We present a methodology for identification of important RF as well as the potentially new once. Materials and Methods: We worked with the dataset of patients hospitalized in the East Slovak Institute of Cardiovascular Diseases in Košice. The file contained 808 records, complete history, laboratory tests, ECG, echocardiography, and selective coronary angiography. We analyzed the importance of variables based on CART, Random forest, and Logistic regression algorithms (binary classification) and propose a new weighted agglomerative attribute importance metric. After selection of potentially important, but less known RF we re-deployed the CART algorithm on selected combinations of risk factors, while the target attribute was divided into six original classes corresponding to the severity of the coronarography finding.Results: Selected important variables based on the proposed metric are in accordance with known results, but also pointed to some potentially relevant RF such as fibrinogen. The experiments confirmed that fibrinogen might have the potential to help determine cardiovascular risk. However, its impact is debatable, but its potential increases if it is potentiated by factors other than HDL. We also concluded that higher HDL levels might have a cardio protective effect. Conclusions: Our study proposed original methodology for identification of important RF and in depth analysis of potentially interesting new RF. Results showed that fibrinogen could be one of the critical risk factors of cardiovascular diseases. Experimental results suggest that there may be others besides traditional RF of CVD, such as the fibrinogen and HDL levels we investigate. Still, studies with a larger patient population are needed to draw significant conclusions.
Fabrication of a 3D supramolecular Cd(II)-coordination polymer as a luminescent sensor for detection of sulfamethazine
Authors: Singh Amita , Shi Chunchun , Zhao Ling , Xu Jia-Jia , Lu Lu , Kumar Abhinav 
Published: June 2022

Abstract
A 3D supramolecular coordinated polymer with formula [Cd2(L)2(bib)(H2O)2] (1) (H2L = 4,4’-(1,2-phenlenebis(methyleneoxy))dibenzoic acid and bib = 1,4- bis(1-imidazoly)benzene), has been synthesized and characterized. A one-dimensional (1D) chain structure of 1 with poly-rotaxane was confirmed by a single crystal X-ray approach. This structure was woven for reaching a 3D supramolecular architecture held by hydrogen-bonded and packing intermolecular interactions between adjacent motifs. The luminescence sensing experiments indicates that it is a fluorescent sensor for the detection of antibiotics in 1. It is a highly sensitive/selective polymer towards sulfamethazine (SMZ) that revealed a low detection limit of 1.05 ppm with a high KSV value of 2.98 × 103 M− 1. Its antibiotics sensing mechanism was also studied by theoretical calculations.
Establishment of an optimized orthotopic bladder cancer model in mice
Authors: Cai Jinming , Xie Zhiwen , Yan Yilin , Huang Zhengnan , Tang Pengfei , Cao Xiangqian , Wang Zeyi , Yang Chenkai , Tan Mingyue , Zhang Fang , Shen Bing 
Published: June 2022

Abstract
Bladder cancer (BC) is one of the most common malignancies of the genitourinary system. Our orthotopic murine BC model provides a tool that is predictable, reproducible, and convenient for exploring tumour initiation, progression, and therapeutic mechanisms. Here, we constructed an optimized orthotopic BC model in male C57BL/6 mice utilizing microsyringes to inoculate them with a murine BC cell line (MB49). Anesthetised mice were inoculated with an MB49 cell suspension (10 µL) at approximately 5×106/mL. The whole process of modelling was observed and monitored every 3 days for 21 days utilizing HE staining and transabdominal ultrasonography (TUS). In this study, the model showed excellent success rates for tumour formation (96.67%) and metastatic rate (89.66%). Compared to the control group (sham operation), mice in the modelling group had serous cachexia, visible haematuresis and weight loss (all P < 0.05). The lungs, liver, ureter and kidneys were found to have tumour metastasis. Moreover, the average survival time (19.73 ± 1.69 d) of modelling mice was significantly shorter than that of the control mice (P < 0.05), which remained alive. Our study established a method using microsyringes to inject murine BC cells into the bladder wall, creating a stable transplantable BC model in mice.
Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella typhimurium
Authors: Wang Weiwei , Ou Jingseng , Ye Hui , Cao Qingyun , Zhang Changming , Dong Zemin , Feng Dingyuan , Jianjun Zuo 
Published: June 2022

Abstract
Background
Salmonella typhimurium challenge causes a huge detriment to chicken production. N-acyl homoserine lactonase (AHLase), a quorum quenching enzyme, potentially inhibits the growth and virulence of Gram-negative bacteria. However, it is unknown whether AHLase can protect chickens against S. typhimurium challenge. This study aimed to evaluate the effects of AHLase on growth performance and intestinal health in broilers challenged by S. typhimurium. A total of 240 one-day-old female crossbred broilers (817C) were randomly divided into 5 groups (6 replicates/group): negative control (NC), positive control (PC), and PC group supplemented with 5, 10 or 20 U/g AHLase. All birds except those in NC were challenged with S. typhimurium from 7 to 9 d of age. Parameter analysis was performed on d 10 and 14.
Results
The reductions (P < 0.05) in body weight (BW) and average daily gain (ADG) in challenged birds were alleviated by AHLase addition especially at 10 U/g. Thus, samples from NC, PC and PC plus 10 U/g AHLase group were selected for further analysis. S. typhimurium challenge impaired (P < 0.05) intestinal morphology, elevated (P < 0.05) ileal inflammatory cytokines (IL-1β and IL-8) expression, and increased (P < 0.05) serum diamine oxidase (DAO) activity on d 10. However, AHLase addition normalized these changes. Gut microbiota analysis on d 10 showed that AHLase reversed the reductions (P < 0.05) in several beneficial bacteria (e.g. Bacilli, Bacillales and Lactobacillales), along with increases (P < 0.05) in certain harmful bacteria (e.g. Proteobacteria, Gammaproteobacteria, Enterobacteriaceae and Escherichia/Shigella) in PC group. Furthermore, AHLase-induced increased beneficial bacteria and decreased harmful bacteria were basically negatively correlated (P < 0.05) with the reductions of ileal IL-1β and IL-8 expression and serum DAO activity, but positively correlated (P < 0.05) with the increased BW and ADG. Functional prediction revealed that AHLase abolished S. typhimurium-induced upregulations (P < 0.05) of certain pathogenicity-related pathways such as lipopolysaccharide biosynthesis, shigellosis, bacterial invasion of epithelial cells and pathogenic Escherichia coli infection of gut microbiota.
Conclusions
Supplemental AHLase attenuated S. typhimurium-induced growth retardation and intestinal disruption in broilers, which could be associated with the observed recovery of gut microbiota dysbiosis.
Obstructive sleep apnea (OSA) is associated with the impairment of beta-cell response to glucose in children and adolescents with obesity
Authors: Umano Giuseppina , Galderisi Alfonso , Aiello Francesca , Martino Mariangela , Camponesco Ornella , Sessa Anna Di , Marzuillo Pierluigi , Papparella Alfonso , Giudice Emanuele Miraglia del 
Published: June 2022

Abstract
Background
The main purpose of the study is to assess the association between obstructive sleep apnea (OSA) and insulin secretion in children with obesity.
Methods
We retrospectively enrolled children and adolescents who attended our pediatric clinic because of obesity and OSA. Glucose homeostasis was assessed through standard 2-hour OGTT. Nocturnal cardio-respiratory polygraphy was performed for OSA diagnosis. Twenty-two patients underwent a 3-hour OGTT test to investigate insulin secretion and sensitivity though the oral-minimal model.
Results
seventy-seven children were included in the study. Based on OSA severity, the cohort was divided into three groups (29 mild, 29 moderate, and 19 severe OSA). The group with mild OSA showed lower 30-minutes glucose(p = 0.01), 60-minutes glucose(p = 0.03), and prevalence of elevated 1-hour glucose (10.4% versus 44.8% in moderate and 31.6% in severe OSA,p = 0.01). The odds for elevated 1-hour plasma glucose was 6.2 fold (95%CI 1.6–23.4) higher in children with moderate and severe OSA compared to mild OSA (p = 0.007) independent of confounders. Spearman test revealed a positive correlation between 30-minutes plasma glucose and AHI (r = 0.31,p = 0.01), ODI (r = 0.31,p = 0.009), and mean desaturation (r = 0.25,p = 0.04). The exploratory study included 22 subjects (7 mild,9 moderate, and 6 severe OSA). The group with mild OSA showed a higher dynamic, static, and total insulin secretion compared to those with moderate and severe OSA (p < 0.0001,p = 0.007,p = 0.007,respectively). AHI was significantly correlated to dynamic insulin secretion (r=-0.48, p = 0.02).
Conclusions
OSA might impair beta-cell function reducing the pool of promptly releasable insulin in obese children and adolescents, in the absence of an effect on insulin sensitivity.
Two-stage optimization layout of grasping points for sheet metal part based on GSA-Kriging model
Authors: Zhu Chenxi , Wan Xiao-Jin , Zhou Zhengjie 
Published: June 2022

Abstract
Automobile flexible sheet metal parts are prone to produce non-negligible grasping deformation, which influences the location accuracy of the part during robot assembly operation, and choosing an appropriate layout of grasping points is an important means to reduce the grasping deformation for sheet metal parts. It is challenging to optimize the layout of grasping points efficiently and accurately because of the large number of robot fingers due to a large size sheet part. In order to improve the optimization efficiency and reduce the computational cost, this paper proposes a two-stage optimization design method for the layout of grasping points based on GSA-Kriging surrogate model. Firstly, the number of robot fingers and their feasible range are determined according to the stability of robotic grasping operation and the degree of deformation at different positions. Then according to the position distribution of grasping points, they are divided into two stages for optimization. Finally, GSA-Kriging surrogate model and gravitational search algorithm (GSA) are used to find the optimal layout of grasping points. In this paper, the layout of grasping points optimization of a certain car door sheet part is utilized as a case to validate the proposed method. The result shows that the GSA-Kriging surrogate model is more accurate and more stable than Kriging and other surrogate models. At the same time, the two-stage optimization method improves the optimization efficiency while reducing the calculation cost and burden.
A novel immune checkpoint score system for prognostic evaluation in pancreatic adenocarcinoma
Authors: Chen Yusheng , Lin Xuan , Zou Xuan , Qian Yunzhen , Liu Yu , Wang Ruijie , Wang Xu , Yu Xianjun , Liu Chen , Cheng He 
Published: June 2022

Abstract
Pancreatic adenocarcinoma (PAAD) remains a lethal malignancy making the detection of novel prognostic biomarkers urgent. Limited studies have investigated the predictive capability of immune checkpoints in PAAD. Here, we presented a risk score system based on three immune checkpoints (OX40/TNFRSF4, TNFSF14 and KIR3DL1) through LASSO and Cox regression analyses for prognostic prediction. The risk score model was an independent prognostic factor and performed well regarding overall survival (OS) predictions among PAAD patients. A nomogram was established to facilitate the risk model application in clinical prognosis. Immune cells including naive B cells, CD8+ T cells and Tregs were negatively correlated with the risk score. Additionally, the risk score was associated with expression of immune checkpoint genes, immunogenic cell death related genes and somatic mutations. Glycolysis processes, IL-2-STAT5, IL-6-STAT3, and mTORC1 signaling pathways were enriched in the high-risk score group. Furthermore, scRNA-seq data confirmed that TNFRSF4, TNFSF14 and KIR3DL1 were expressed on immune cells in PAAD samples. We then identified OX40 as an independent prognosis-related gene, and a higher OX40 expression was associated with increased survival rate and immune environment change. In 84 PAAD clinical specimens collected from our center, we confirmed that higher OX40 mRNA expression levels were related to a good prognosis. The protein expression of OX40 on tumor-infiltrating immune cells (TIICs), endothelial cells and tumor cells was verified in PAAD tissues by immunohistochemistry (IHC) method. Overall, our findings strongly suggested that the three-immune checkpoint score system might be useful in the prognosis and design of personalized treatments for PAAD patients. Finally, we identified OX40 as an independent potential biomarker for PAAD prognosis prediction.
Developmental assessment of infants with congenital heart disease
Authors: Sheta Sahar , Amin Omnia R , Elkateb Ahmed , Toukhy Noha El , Sayed Shaimaa 
Published: June 2022

Abstract
Background: Recently, marked advances in surgical and medical care for infants with congenital heart disease led to a growing population with a high risk of developmental delay. We aimed to identify developmental delay in these infants and its risk factors. Methods: A prospective cross-sectional study on 100 infants with congenital heart disease. Developmental assessment was done by using Vineland Adaptive Behavior Scale. We searched for risk factors for developmental delay in these infants. Correlations were conducted between the degree of developmental delay and the potential risk factors. To our knowledge, this study has not been conducted in developing countries before.Results: The median age of the study group is 12.5 months, while by using Vineland Adaptive Behavior Scale; it is equivalent to 9.5 months. There was a statistically significant developmental affection in infant who had risk factors.Conclusions: Developmental delay is a common complication in infants with congenital heart disease. It has many risk factors; some of them could be modifiable. Thus, early screening for developmental delay and its risk factors are of a great value. This could help in applying preventive measures and early interventional programs. Vineland Adaptive Behavior Scale is a reliable tool in determination of developmental delay.
Muscle and adjacent subcutaneous fat thicknesses of the gastrocnemius medialis and rectus femoris and the relationship with countermovement jump and v-cut test performance in young elite basketball players
Authors: Lisón Juan Francisco , García-Herreros Sergio , Ricart Borja , Godoy Eduardo Jorge , Nozal Sara , Cotolí-Suárez Pedro , Jordán-López Jaime , Amer-Cuenca Juan José , Salvador-Coloma Pablo 
Published: June 2022

Abstract
The countermovement jump (CMJ), the V-cut test, the muscle thickness (MT) and the adjacent subcutaneous fat thickness (SFT) of the gastrocnemius medialis (GM) and rectus femoris (RF) are important physiological indicators for success in basketball. The aims of this study were to obtain normative data regarding CMJ and V-cutperformance and ultrasound measurements, evaluate between-age-category and between-gender differences in these data and examine the relationships between physical tests and ultrasound measurements. The measurements were recorded in a sample of 131 elite basketball players (66 males) who played in three age-categories (U14, U16, or U18).We performed two-way analysis of covariance tests and age-adjusted partial correlation analyses. U16 and U18 males showed better performance in the CMJ and V-cut tests and lower GM and RF SFT compared to the U14 males (p ≤ .001) and to age-category equivalent female players (p ≤ .001). Comparisons between the females did not show significant differences in any of the study variables. V-cut and GMSFT variables explained 53.3% of the variation for the CMJ result in males (p < .01). This study reports normative data from CMJ and V-cut tests and ultrasound measurements of different age-category male and female elite youth basketball players. Furthermore, it is the first to show the association and predictive role of subcutaneous fat thickness in physical performance.
Benchmarking integration of single-cell differential expression
Authors: Nguyen Hai , Baik Bukyung , Yoon Sora , Park Taesung , Nam Dougu 
Published: June 2022

Abstract
Integration of single-cell RNA sequencing (scRNA-seq) data between different samples has been a major challenge for analyzing cell populations. However, strategies to integrate differential expression (DE) analysis of scRNA-seq data remain underinvestigated. Here, we benchmarked 41 methods for integrative DE analysis of scRNA-seq data. Batch-effects, sparsity of data, and heterogeneity of samples substantially impacted the performance of DE analysis. Several methods that yielded high performances were suggested based on various simulations and real data analyses. In particular, the bulk RNA-seq tool edgeR incorporating the observation weights and the scRNA-seq tool MAST showed overall good performances. Remarkably, analysis for a specific cell type outperformed that of large-scale bulk sample data in prioritizing disease-related genes and pathways.
Investigation on the behavior of bridge piers considering rocking isolation constructed on non-plastic silts and sands using 1g shaking table tests
Authors: Irani Arash Esmatkhah , Hajialilue-Bonab Masoud , Sarand Fariba Behrooz , Katebi Hooshang 
Published: June 2022

Abstract
Occurred damages on the bridge piers during earthquakes lead to significant financial losses worldwide every year and can cause social problems by disrupting traffic flow and transportation services. Rocking isolation of foundations is one of the damage reduction approaches to avoid structural damage on piers by transferring plastic hinges from piers to underlaying soil media. However, the behavior of rocking foundations on sands and clays has been studied comprehensively in past years; the behavior of these foundations on non-plastic silts has not been investigated well in the literature. In this research, the characteristic seismic behavior of a bridge pier considering rocking isolation is evaluated using small-scale physical modeling tests. To this aim, eight shaking table tests are conducted where both sandy and silty material are employed as the soil media. In addition to the effects of the underlying soil, the effects of the critical contact area ratio of the foundation and frequency of input motions are evaluated. Achieved results indicate that the considered bridge pier shows the same behavior trend for underlying silty soil and sandy one. However, because of the frequency-dependent behavior of silts and larger damping properties of such material, pier attracts lower accelerations and higher moments in non-plastic silty soil. Therefore, the achieved results show that the proposed design approaches of rocking foundations that are mostly extracted based on experimental studies on sands (or rarely on clay) can lead to non-conservative designs in silty soils.