bluuebunny/crossref_metadata_2025_split huggingface dataset:
Análise da efetividade da terapia de exposição à realidade virtual no equilíbrio de portadores de Parkinson: revisão integrativa
Authors: Pedroso Paulo César Sales , Ferreira Juliane Marcelle da Silva , Sá Mariana dos Anjos Fuertado de 
Published: March 2022

A Terapia de exposição à realidade virtual diz respeito a uma técnica que permite a interação entre o indivíduo e um sistema computacional que recria a realidade numa interface virtual, oferecendo treinamento cognitivo e motor concomitantemente. Desta forma a fisioterapia está cada dia mais utilizando este método para o tratamento da DP, o que vem sendo demonstrado em diversos estudos. Diante disto, o presente trabalho teve como objetivo conhecer os efeitos da gameterapia no equilíbrio de indivíduos com doença de Parkinson através de uma revisão integrativa da literatura, sendo utilizadas as bases de dados BVS (Biblioteca Virtual em Saúde), SciELO (Scientific Electronic Library Online), PubMed e PEDro, com a busca de estudos publicados entre os anos de 2016 e 2021, utilizando descritores de acordo com o DeCS/MeSH: “Doença de Parkinson” AND “Terapia de exposição a realidade virtual” OR “ Gameterapia” OR “Vídeo Game” e suas respectivas traduções para a língua inglesa. Após analise metodológica foram incluídos 05 artigos que condiziam com o objetivo deste estudo. Os resultados demonstraram que a reabilitação de portadores de Parkinson por meio da terapia de exposição à realidade virtual mostrou-se como ferramenta eficaz na melhora do equilíbrio corporal e da capacidade funcional, demonstrando potencial na redução do risco de quedas, aumentando a autoconfiança e melhorando a qualidade de vida dos pacientes, contudo, não fica claro se esta modalidade seja mais efetiva que a fisioterapia convencional. Ressalta-se a necessidade de mais estudos com maior rigor metodológico.
RELASI QUADRUPLE HELIX DALAM PENGEMBANGAN ORGANISASI MASYARAKAT SIPIL PADA BADAN KESATUAN BANGSA DAN POLITIK KABUPATEN KOLAKA
Authors: Langka Meity , Suaib Eka , Alam Syamsul 
Published: March 2022

Penelitian ini bertujuan untuk mendeskripsikan dan menganalisis relasi Quadruple Helix dalam pengembangan organisasi masyarakat sipil di Badan Kesatuan Bangsa dan Politik Kabupaten Kolaka . Peneltian ini menggunakan pendekatan kualitatif dengan informan yang berjumlah sebanyak 9 orang yang dipilih secara purposive sampling. Penelitian ini berfokus pada model Quadruple Helix dalam pengembangan organisasi masyarakat sipil. Pengumpualn data dalam penelitian ini melalui wawancara mendalam dan dokumentasi. Data yang diperoleh tersebut dianalisis secara kualitaif melalui analisis datan interaktif yang terdiri dari reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa relasi Quadruple Helix dalam pengembangan organisasi masyarakat sipil pada Badan Kesatuan Bangsa dan Politik Kabupaten Kolaka yaitu Pemerintah berperan sebagai regulator sekaligus berperan sebagai kontroler yang memiliki peraturan dan tanggung jawab dalam mengembangkan objek., industri berperan sebagai enabler yang menghadirkan infrastruktur, dengan mendukung perubahan pada sumber daya manusia bussines dalam kolaboratif governance. Akademisi pada model Quadruple Helix berperan sebagai konseptor. Seperti melakukan standarisasi proses pada kegiatan yang dilakukan serta sertifikasi dan ketrampilan sumber daya manusia dan Media pada model Quadruple Helix berperan sebagai expender, dalam hal ini media berperan dalam mendukung publikasi dalam promosi dan membuat brand image. dalam program pengembangan kerjasama tersebut.Kata Kunci: Relasi, Quadruple Helix , Pengembangan Organisasi Masyarakat Sipil
Application of Magnetically Assisted Reactors for Modulation of Growth and Pyocyanin Production by Pseudomonas aeruginosa
Authors: Jabłońska Joanna , Dubrowska Kamila , Augustyniak Adrian , Kordas Marian , Rakoczy Rafał 
Published: March 2022

Pseudomonas aeruginosa is a producer of desired secondary metabolites, including pyocyanin. Potential uses of this pigment urge a search for improved production methods. Recent trends in bioprocessing show the potential of the use of electromagnetic fields (EMFs) to influence the growth of microorganisms and even modulate the concentration of bioproducts. Here, we aimed at assessing the influence of rotating magnetic field (RMF) and static magnetic field (SMF) on pyocyanin production, growth rate, and respiration of P. aeruginosa. Moreover, exposure time to EMFs (2, 6, and 12 h) and culture volume (10 and 50 ml) were initially assessed. P. aeruginosa was cultivated in magnetically assisted reactors with 5 and 50 Hz RMF (magnetic induction of 24.32 and 42.64 mT, respectively) and SMF (−17.37 mT). Growth kinetics was assessed with Gompertz equation. The viability was tested using resazurin assay, whereas pyocyanin production by chloroform-HCl methodology. The growth of P. aeruginosa was slightly stimulated by exposure to a RMF with 50 Hz (108% related to the control) and significantly by SMF (132% related to the control), while RMF 5 Hz exposure prolonged the time of inflection (in comparison to RMF 50 Hz and SMF). The 6-h exposure to EMFs resulted in the highest pyocyanin production in comparison to the control, indicating a relationship between exposure time and product concentration. Moreover, cultures led in smaller volumes produced more pyocyanin. Our findings show that the use of different EMF types, frequency, and exposition time and volume could be used interchangeably to obtain different bioprocess aims.
A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576
Authors: Liu Jun , Zhang Xinyu , Liu Guodong , Zhao Guoqin , Fang Xiaoran , Song Xin 
Published: March 2022

Sophorolipids (SLs), an important biosurfactant produced by S. bombicola, were one of the most potential substitutes for chemical surfactants. Few reports on the transcriptional regulation of SLs synthesis and the engineered strains with high-yield SLs were available. In this study, a Rim9-like protein (Rlp) and three transcription factors (ztf1, leu3, gcl) were mined and analyzed, and a progressive enhancement of SLs production was achieved through cumulative knockouts of three genes. The sophorolipid production of ΔrlpΔleu3Δztf1 reached 97.44 g/L, increased by 50.51% than that of the wild-type strain. Compared with the wild-type strain, the flow of glucose to SLs synthesis pathways was increased, and the synthesis of branched-chain amino acids was reduced in ΔrlpΔleu3Δztf1. The amount of UDP-glucose, the substrate for two glycosyltransferases, also increased, and the expression level of the key genes sble and UGPase for SLs synthesis increased by 2.2 times, respectively. The multiple-gene knockout strategy was proved to be highly effective to construct the engineered strain with high-yield SLs production, and this strain was a superior strain for industrial fermentation of SLs and reduced SLs production costs.
The Effect of Silicate Ions on the Separation of Lithium From Geothermal Fluid
Authors: Lee Junbeum , Chung Eunhyea 
Published: March 2022

In an enhanced geothermal system (EGS), geothermal energy in rocks with insufficient permeability or fluid saturation can be used by creating artificial geothermal reservoirs. Generally, EGS geothermal fluid contains high concentrations of total dissolved solids that originated from various geochemical reactions between the fluid in the reservoir and the minerals in the rock. For example, the concentration of lithium ions are measured approximately 150 mg/L, and several researchers have focused on the recovery of lithium in the geothermal fluid using various methods, one of which is liquid extraction. Solvent extraction has been used to recover lithium from various sources, and successful recovery efficiency have been attained. However, the geothermal fluid in EGS reservoirs contains high concentrations of SiO2, which might inhibit the selective recovery of lithium. Thus, in this study, two consecutive stages of solvent extraction were used to separate the lithium from the geothermal fluid that contained different concentrations of SiO2 ions. The divalent ions were removed in the first stage, and the lithium ions were extracted effectively in the second stage. The SiO2 inhibits the selective recovery of lithium in the first stage to a greater extent than it does in the second stage. The spectroscopy data shows a decrease of the organic solvents main functional group (P=O &amp; P-O-H) absorbance that reacts with the metal ions of the geothermal water after extraction however the intensity difference was reduced as the SiO2 concentrations increases. Silicate ions can be problematic due to the formation of scaling in EGSs, so controlling its concentration in the geothermal reservoir would be beneficial for the long-term operation of EGSs and for the successful recovery of valuable metal resources from EGS reservoirs.
Radioactive Iodine for the Treatment of Subclinical Thyrotoxicosis Grade 1 and 2: Outcome of up to 18-Year Follow Up
Authors: Phowira Jason , Coffey Katherine L. , Bartholomew Peter H. , Vennart Nicholas , Moreira Matheus , Emerson Hannah , Kennedy David , Weaver Jolanta U. 
Published: March 2022

BackgroundSubclinical thyrotoxicosis (SCT) is associated with significant morbidity and mortality, specifically increased risk of atrial fibrillation and cardiovascular death. The management is ill-defined due to the scarcity of randomised controlled studies. Some clinicians recommend radioiodine (RAI) treatment however its long-term outcome is unknown. Therefore, further data is needed to provide robust evidence-based guidelines.MethodsA prospective, single-protocol analysis of the outcome of SCT patients (Grade 1; 0.1-0.4 mIU/L and Grade 2; &lt;0.1 mIU/L) treated with mean dose of 427 MBq of I131, followed up for up to 18 years. Thyroid function tests were measured at 4-6 weeks, 3-, 6-, and 12-months post-RAI, and annually thereafter. Cure was defined as achieving a euthyroid/hypothyroid state.ResultsSeventy-eight patients with a median age of 68 years (range 36-84) and varying aetiology [55 toxic multinodular goitre (TMNG), 10 toxic nodule (TN) and 13 Graves’ disease (GD)] were followed up for a median period of 7.5 years (range 1-18). The cure rate was 100%. The rates of hypothyroidism in TMNG, TN and GD were 23.6%, 30% and 38.5% respectively. The median time to hypothyroidism was 6 and 12 months in GD and TMNG/TN respectively. No differences in outcome between Grade 1 versus Grade 2 were observed.ConclusionRAI using single mean dose of 427 MBq is effective and safe, irrespective of aetiology or grade of TSH suppression. GD patients become hypothyroid within the first year, whilst TMNG/TN for up to 9-years. Thus after 12 months of follow up, annual thyroid function monitoring is advised.
Elevated Serum Leptin Levels as a Predictive Marker for Polycystic Ovary Syndrome
Authors: Peng Yuanyuan , Yang Hongyue , Song Jiahui , Feng Di , Na Zhijing , Jiang Hongyu , Meng Yaxin , Shi Bei , Li Da 
Published: March 2022

BackgroundLeptin may have important implications in polycystic ovary syndrome (PCOS)-related metabolic disorders. However, the changes in serum leptin levels in patients with PCOS and its predictive value for PCOS remain obscure. We intend to analyze the association between leptin and PCOS in this study.Materials and MethodsThe study comprised 89 patients with PCOS and 139 individuals without PCOS. Each group was stratified as either normal- or hyper-fasting serum insulin (FSI), and lean or overweight/obese; and the patients were further categorized as normal- or hyper-androgenic. The validity of leptin toward the diagnosis of PCOS, or leptin combined with total testosterone, dehydroepiandrosterone sulfate (DHEAS), and free testosterone was estimated by receiver operating characteristic (ROC) curves, and correlations between paired variables was estimated by Spearman’s rank correlation coefficient. Associations between the clinical and metabolic variables and PCOS were analyzed via logistic regression.ResultsThe serum leptin levels of patients with PCOS were significantly higher than that of the control, and especially the PCOS in hyper-FSI, hyperandrogenimic and overweight/obese subgroups. The area under the ROC curve (AUC) of leptin was 74%, with cutoff value, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) 11.58 ng/mL, 77.5%, 62.6%, 57.0%, and 81.3%, respectively. Combined leptin and anti-Müllerian hormone (AMH) had the highest AUC (92.3%), excellent sensitivity (93.3%), moderate specificity (78.3%), PPV (73.5%) and NPV (94.8%). Serum leptin levels of the patients were correlated with the FSI, fasting plasma glucose (FPG), homeostasis model assessment of insulin resistance (HOMA-IR), body mass index (BMI), and total testosterone levels. Elevated serum leptin was associated with a high risk of PCOS [P = 0.015; OR (95% CI) 1.128 (1.024–1.244)].ConclusionSubstantially elevated serum leptin is significantly associated with PCOS. These findings warrant further investigations into the function of leptin in the pathogenesis of PCOS.
Diabetes Mellitus Predicts Weight Gain After Surgery in Patients With Acromegaly
Authors: Jang Han Na , Kim Yong Hwy , Kim Jung Hee 
Published: March 2022

ObjectiveMetabolic complications are common in patients with acromegaly. However, this occasionally does not improve post-surgery and may be related to postoperative weight gain. We aimed to investigate the postoperative weight change and factors associated with postoperative weight gain in patients with acromegaly.Design and MethodsOverall, 113 consecutive patients with body weight records pre- and 3–6 months post-surgery between October 2009 and March 2021 were enrolled. Patients were divided into three groups: weight loss (weight decrease ≥3%), stable, and weight gain (weight increase ≥3%). Hormone status, metabolic comorbidities, and anthropometric parameters were compared between the groups.ResultsAmong 113 patients, 29 (25.7%) and 26 (23.0%) patients lost and gained weight, respectively, post-surgery. There were no significant differences in baseline characteristics, including age at diagnosis, sex, body mass index, and growth hormone levels among the three groups. The prevalence of diabetes mellitus at diagnosis was significantly higher in the weight gain group than in the other groups. Patients with diabetes (n=22) had a 5.2-fold higher risk of postoperative weight gain than those with normal glucose tolerance (n=37) (P=0.006). In the diabetes mellitus group, the percentage lean mass decreased (-4.5 [-6.6–2.0]%, P=0.002), and the percentage fat mass significantly increased post-surgery (18.0 [4.6–36.6]%, P=0.003), whereas the normal glucose tolerance group did not show body composition changes post-surgery.ConclusionIn patients with acromegaly, 23% experienced ≥3% weight gain post-surgery. Diabetes mellitus at diagnosis is a significant predictor of weight and fat gain post-surgery.
Eosinophilia and Lung Cancer: Analysis From Real-World Data and Mendelian Randomization Study
Authors: Wang Zhufeng , Chen Bigui , Fu Yu , Ou Changxing , Rong Qiuping , Kong Xuetao , Xu Wei , Deng Yangqing , Jiang Mei , Xie Jiaxing 
Published: March 2022

Background and ObjectiveGrowing evidence added to the results from observational studies of lung cancer patients exhibiting eosinophilia. However, whether eosinophils contributed to tumor immune surveillance or neoplastic evolution was unknown. This study aimed to analyze the causal association between eosinophilia and lung cancer.MethodsThe causal effect of eosinophil count on lung cancer from a genome-wide association study (GWAS) was investigated using the two-sample Mendelian randomization (MR) method. Secondary results according to different histological subtypes of lung cancer were also implemented. Meanwhile, we compared the measured levels of blood eosinophil counts among different subtypes of lung cancer from real-world data.ResultsThe median absolute eosinophilic count (unit: 109/L) [median (min, max): Lung adenocarcinoma 0.7 (0.5, 15); Squamous cell lung cancer 0.7 (0.5, 1.3); Small cell lung cancer 0.7 (0.6, 1.3); p = 0.96] and the median eosinophil to leukocyte ratio [median (min, max): Lung adenocarcinoma 8.7% (2.1, 42.2%); Squamous cell lung cancer 9.3% (4.1, 17.7%); Small cell lung cancer 8.9% (5.1, 24.1%); p = 0.91] were similar among different histological subtypes of lung cancer. MR methods indicated that eosinophilia may provide 28% higher risk for squamous cell lung cancer in East Asian [Weighted median method: odds ratio (OR) = 1.28, 95% CI: 1.04–1.57, p = 0.02].ConclusionOur study suggested that eosinophilia may be a potential causal risk factor in the progression of squamous cell lung cancer in East Asian.
Intravenous Lidocaine and Ketamine Infusions for Headache Disorders: A Retrospective Cohort Study
Authors: Ray Jason C. , Cheng Shuli , Tsan Kirsten , Hussain Hassan , Stark Richard J. , Matharu Manjit S. , Hutton Elspeth 
Published: March 2022

IntroductionThe use of lidocaine (lignocaine) and ketamine infusion in the inpatient treatment of patients with headache disorders is supported by small case series. We undertook a retrospective cohort study in order to assess the efficacy, duration and safety of lidocaine and ketamine infusions.MethodsPatients admitted between 01/01/2018 and 31/07/2021 were identified by ICD code and electronic prescription. Efficacy of infusion was determined by reduction in visual analog score (VAS), and patient demographics were collected from review of the hospital electronic medical record.ResultsThrough the study period, 83 infusions (50 lidocaine, 33 ketamine) were initiated for a headache disorder (77 migraine, three NDPH, two SUNCT, one cluster headache). In migraine, lidocaine infusion achieved a ≥50% reduction in pain in 51.1% over a mean 6.2 days (SD 2.4). Ketamine infusion was associated with a ≥50% reduction in pain in 34.4% over a mean 5.1 days (SD 1.5). Side effects were observed in 32 and 42.4% respectively. Infusion for medication overuse headache (MOH) led to successful withdrawal of analgesia in 61.1% of lidocaine, and 41.7% of ketamine infusions.ConclusionLidocaine and ketamine infusions are an efficacious inpatient treatment for headache disorders, however associated with prolonged length-of-stay and possible side-effects.
The Evolution of Clinicopathological Diagnostic Features of Upper Tract Urothelial Carcinoma in China: A Summary of 2561 Cases in the Last 20 Years
Authors: Xu Chunru , Yuan Changwei , Zhang Cuijian , Fang Dong , Yu Yanfei , Wang Xiang , Li Zhihua , Wang Yan , Tang Qi , Xiong Gengyan , Zhang Lei , He Zhisong , Lin Jian , Zhou Liqun , Li Xuesong 
Published: March 2022

ObjectivesTo summarize the clinicopathological diagnostic features and evolutionary trends of upper tract urothelial carcinoma (UTUC) in China over the past 20 years.MethodsAll patients diagnosed with upper tract urothelial carcinoma in the Peking University First Hospital from 2001 to 2020 were retrospectively collected. Data were divided into two groups (2001-2010 and 2011-2020) according to the date of diagnosis. Statistical analysis was done with the SPSS V22.0. Chi-square analysis and t-test were adopted to analyze depending on the data type. Subgroup analysis based on 5 years was used for visualization to present trends. Both Kaplan-Meier curve and Cox regression were used for univariate and multivariate survival analysis.ResultsThe study included 2561 cases diagnosed with upper tract urothelial carcinoma in total. Compared with the first decade (2001-2010), patients of the second decades (2011-2020) had elder mean age (66.65 versus 67.59, years, p=0.025), higher male proportion (43.5% versus 49.0%, p=0.034), lower incidence of renal pelvic tumors (53.4% versus 45.8%, p&lt;0.001) and multifocality (18.6% versus 12.0%, p&lt;0.001), higher incidence of ureteral tumors (52.2% versus 60.9%, p&lt;0.001).In recent ten years, the incidence of muscle-invasive urothelial carcinoma (pT2+) decreased significantly (64.4% versus 54.9%, p&lt;0.001),and the mean size of renal pelvic tumors increased(3.46 versus 3.73, cm, p=0.043). The size of the ureteral tumor, the histopathologic grade showed no significant change. The prognostic analysis based on 709 patients regularly followed at our center revealed that the male gender and G3 histopathological grade were independent risk factors for poorer prognosis in patients with UTUC.ConclusionIn the past 20 years, the clinicopathological diagnostic features of upper tract urothelial carcinoma in the Chinese population has changed significantly, suggesting an increased risk of a poorer prognosis for UTUC. This trend may be related to updating diagnostic techniques and self-monitoring awareness. However, we need more high-grade, multicenter trials to verify it in the future.
Adipogenic Transdifferentiation and Regulatory Factors Promote the Progression and the Immunotherapy Response of Renal Cell Carcinoma: Insights From Integrative Analysis
Authors: Wang Shuai , Wei Xiyi , Ji Chengjian , Wang Yichun , Zhang Xi , Cong Rong , Song Ninghong 
Published: March 2022

BackgroundAdipogenic transdifferentiation was an important carcinogenic factor in various tumors, while studies on its role in clear cell renal cell carcinoma (ccRCC) were still relatively few. This study aimed to investigate its prognostic value and mechanism of action in ccRCC.MethodsGene expression profiles and clinical data of ccRCC patients were obtained from The Cancer Genome Atlas database. Nonnegative matrix factorization was used for clustering. Gene set variation analysis (GSVA) and gene set enrichment analysis (GSEA) were used to analyze the pathways and biological process activities. single-sample GSEA (ssGSEA) was utilized to quantify the relative abundance of each immune cell. Tumor Immune Estimation Resource (TIMER) was used to evaluate the proportion of various immune infiltrating cells across diverse cancer types. Real-Time PCR was performed to examine the gene expression. R software was utilized to analyze the expression and prognostic role of genes in ccRCC.ResultsA total of 49 adipose-related genes (ARGs) were screened for differential expression between normal and ccRCC tissues. Based on differentially expressed ARGs, patients with ccRCC were divided into two adipose subtypes with different clinical, molecular, and pathway characteristics. Patients in cluster A exhibited more advanced pathological stages, higher expressions of RARRES2 and immune checkpoint genes, higher immune infiltration scores, and less nutrient metabolism pathways. Adipose differentiation index (ADI) was constructed according to the above ARGs and survival data, and its robustness and accuracy was validated in different cohorts. In addition, it was found that the expression of ARGs was associated with immune cell infiltration and immune checkpoint in ccRCC, among which GBP2 was thought to be the most relevant gene to the tumor immune microenvironment and play a potential role in carcinogenesis and invasion of tumor cells.ConclusionOur analysis revealed the consistency of higher adipogenic transdifferentiation of tumor cells with worse clinical outcomes in ccRCC. The 16-mRNA signature could predict the prognosis of ccRCC patients with high accuracy. ARGs such as GBP2 might shed light on the development of novel biomarkers and immunotherapies of ccRCC.