bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postoperative In-Stent Thrombus Formation Following Frozen Elephant Trunk Total Arch Repair
Authors: Walter Tim , Berger Tim , Kondov Stoyan , Gottardi Roman , Benk Julia , Rylski Bartosz , Czerny Martin , Kreibich Maximilian 
Published: June 2022

ObjectivesOur aim was to investigate the occurrence and clinical consequence of postoperative in-stent thrombus formation following the frozen elephant trunk (FET) procedure.MethodsPostoperative computed tomography angiography (CTA) scans of all 304 patients following the FET procedure between 04/2014 and 11/2021 were analysed retrospectively. Thrombus size and location were assessed in multiplanar reconstruction using IMPAX EE (Agfa HealthCare N.V., Morstel, Belgium) software. Patients’ characteristics and clinical outcomes were evaluated between patients with and without thrombus formation.ResultsDuring the study period, we detected a new postoperative in-stent thrombus in 19 patients (6%). These patients were significantly older (p = 0.009), predominantly female (p = 0.002) and were more commonly treated for aortic aneurysms (p = 0.001). In 15 patients (79%), the thrombi were located in the distal half of the FET stent-graft. Thrombus size was 18.9 mm (first quartile: 12.1; third quartile: 33.2). Distal embolisation occurred in 4 patients (21%) causing one in-hospital death caused by severe visceral ischaemia. Therapeutic anticoagulation was initiated in all patients. Overstenting with a conventional stent-graft placed within the FET stent-graft was the treatment in 2 patients (11%). Outcomes were comparable both groups. Female sex (p = 0.005; OR: 4.289) and an aortic aneurysm (p = 0.023; OR: 5.198) were identified as significant predictors for thrombus development.ConclusionPostoperative new thrombus formation within the FET stent-graft is a new, rare, but clinically highly relevant event. The embolisation of these thrombi can result in dismal postoperative outcomes. More research is therefore required to better identify patients at risk and improve perioperative treatment.
Adaptability Analysis of Full Height Mining at One Time of Deep Soft Thick Coal Seam
Authors: Li Qinghai , Wang Haonan , Zheng Xuejun , Li Kaixin , Ji Yonghu , Wang Zijun 
Published: June 2022

With the improvement of technology and equipment, it is preferred to adopt full-height mining at one time when conditions permit. According to the specific geological conditions of no. 3 coal seam in Lilou Coal Industry, the feasibility of full-height mining and top-caving mining method for soft thick coal seam with large buried depth is analyzed by means of theoretical analysis, numerical simulation and field application analysis, which provides basis for the subsequent improvement of field mining method. Through the establishment of cantilever beam mechanical model of basic roof, the influence of two mining methods on the energy response of basic roof is analyzed. Based on the energy storage characteristics of coal seam, roof and floor measured in the field, the intensity of energy released from disturbed strata by two coal mining methods is analyzed. PFC2D numerical simulation was used to compare the roof failure of the two mining methods, to monitor the change of the stress in the coal seam after excavation, and to calculate the coal seam burial depth suitable for a full mining height by adjusting the in-situ stress. The results show that compared with top-coal mining, the elastic strain energy accumulated in the full-height roof beam is more, and the energy release intensity of disturbed rock is greater. The roof crack extension height is 9.1 m and the coal wall failure depth is 5.46 m under the condition of full mining height at one time. The roof crack extension height is 1.19 m and the coal wall failure depth is 2.19 m under the caving coal mining method. In the caving coal mining method, the stress level of the original rock is restored at 3 m in front of the coal wall, and the stress level of the original rock is restored at 5 m in front of the coal wall when the full mining height is once taken. It is safer to adopt full-height mining method when the buried depth of coal seam is less than 380 m. The research results can provide reference for mining soft thick coal seam with large buried depth.
Pregnancy Outcomes of Different Endometrial Preparation in Patients With a History of Cesarean Section
Authors: Gan Run Xin , Li Yuan , Song Juan , Wen Quan , Lu Guang Xiu , Lin Ge , Gong Fei 
Published: June 2022

ObjectiveTo investigate the efficacies of three cycle regimens in women receiving frozen embryo transfer with a history of cesarean section: natural cycle treatment, hormone replacement therapy and treatment with gonadotropin-releasing hormone agonist.DesignRetrospective cohort study.Methodspatients (N = 6,159) with a history of caesarean section who fulfilled the inclusion criteria were enrolled in the study from January 2014 to December 2019 at the CITIC-Xiangya Hospital of Reproduction and Genetics. Reproductive outcomes of patients in the natural cycle (n = 4,306) versus hormone replacement therapy (n = 1,007) versus gonadotropin-releasing hormone agonist + hormone replacement therapy groups (n = 846) were compared. Continuous data were analyzed using Student’s t-test, and categorical variables were analyzed using the χ2 test. Multivariable logistic regression was used to evaluate the possible relationships between the types of endometrial preparation and pregnancy outcomes after adjusting for confounding factors.ResultsThe unadjusted odds of the miscarriage rate of singleton pregnancies were significantly higher in the hormone replacement therapy compared with the natural cycle (25.5% versus 20.4%, respectively). After adjusting for possible confounding factors, the early miscarriage rate and the miscarriage rate of singleton pregnancies remained significantly higher in the hormone replacement therapy than the natural cycle. The clinical pregnancy rates in the natural cycle, hormone replacement therapy and gonadotropin- releasing hormone agonist + hormone replacement therapy of women with a history of cesarean section was 48.8%, 48% and 47.1%, respectively, and the live birth rates were 37%, 34.1% and 35.7%, respectively.ConclusionsIn women undergoing frozen embryo transfer with a history of cesarean section, hormone replacement therapy for endometrial preparation was associated with a higher early miscarriage rate, albeit after statistical adjustment for confounding factors. However, the risk observed was little and did not influence the overall reproductive performances.
Total Flavonoids of Drynariae Rhizoma Improve Glucocorticoid-Induced Osteoporosis of Rats: UHPLC-MS-Based Qualitative Analysis, Network Pharmacology Strategy and Pharmacodynamic Validation
Authors: Zhang Fangqing , Li Qiuyue , Wu Jiashuo , Ruan Haonan , Sun Chuanrui , Zhu Jia , Song Qinghui , Wei Xu , Shi Yue , Zhu Liguo 
Published: June 2022

BackgroundGlucocorticoid-induced osteoporosis (GIOP) is a common form of secondary osteoporosis caused by the protracted or a large dosage of glucocorticoids (GCs). Total flavonoids of Drynariae rhizoma (TFDR) have been widely used in treating postmenopausal osteoporosis (POP). However, their therapeutic effects and potential mechanism against GIOP have not been fully elucidated.MethodsUltra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESIQ-TOF-MS) experiments were performed for qualitative analysis. We performed hematoxylin-eosin (HE) staining and microcomputed tomography (micro-CT) analysis to detect the changes in bone microstructure. The changes in biochemical parameters in the serum samples were determined by performing an enzyme-linked immunosorbent assay (ELISA). The prediction results of network pharmacology were verified via quantitative real-time polymerase chain reaction (qRT-PCR) to elucidate the potential mechanism of TFDR against GIOP.ResultsA total of 191 ingredients were identified in vitro and 48 ingredients in vivo. In the in-vivo experiment, the levels of the serum total cholesterol (TC), the serum triglyceride (TG), Leptin (LEP), osteocalcin (OC), osteoprotegerin (OPG), bone morphogenetic protein-2 (BMP-2), propeptide of type I procollagen (PINP), tartrate-resistant acid phosphatase (TRACP) and type-I collagen carboxy-terminal peptide (CTX-1) in the TFDR group significantly changed compared with those in the GIOP group. Moreover, the TFDR group showed an improvement in bone mineral density and bone microstructure. Based on the results of network pharmacology analysis, 67 core targets were selected to construct the network and perform PPI analysis as well as biological enrichment analysis. Five of the targets with high “degree value” had differential gene expression between groups using qRT-PCR.ConclusionTFDR, which may play a crucial role between adipose metabolism and bone metabolism, may be a novel remedy for the prevention and clinical treatment of GIOP.
Maximizing Molecular Data From Low-Quality Fluid-Preserved Specimens in Natural History Collections
Authors: Bernstein Justin M. , Ruane Sara 
Published: June 2022

Over the past decade, museum genomics studies have focused on obtaining DNA of sufficient quality and quantity for sequencing from fluid-preserved natural history specimens, primarily to be used in systematic studies. While these studies have opened windows to evolutionary and biodiversity knowledge of many species worldwide, published works often focus on the success of these DNA sequencing efforts, which is undoubtedly less common than obtaining minimal or sometimes no DNA or unusable sequence data from specimens in natural history collections. Here, we attempt to obtain and sequence DNA extracts from 115 fresh and 41 degraded samples of homalopsid snakes, as well as from two degraded samples of a poorly known snake,Hydrablabes periops.Hydrablabeshas been suggested to belong to at least two different families (Natricidae and Homalopsidae) and with no fresh tissues known to be available, intractable museum specimens currently provide the only opportunity to determine this snake’s taxonomic affinity. Although our aim was to generate a target-capture dataset for these samples, to be included in a broader phylogenetic study, results were less than ideal due to large amounts of missing data, especially using the same downstream methods as with standard, high-quality samples. However, rather than discount results entirely, we used mapping methods with references and pseudoreferences, along with phylogenetic analyses, to maximize any usable molecular data from our sequencing efforts, identify the taxonomic affinity ofH. periops, and compare sequencing success between fresh and degraded tissue samples. This resulted in largely complete mitochondrial genomes for five specimens and hundreds to thousands of nuclear loci (ultra-conserved loci, anchored-hybrid enrichment loci, and a variety of loci frequently used in squamate phylogenetic studies) from fluid-preserved snakes, including a specimen ofH. periopsfrom the Field Museum of Natural History collection. We combined ourH. periopsdata with previously published genomic and Sanger-sequenced datasets to confirm the familial designation of this taxon, reject previous taxonomic hypotheses, and make biogeographic inferences forHydrablabes. A secondH. periopsspecimen, despite being seemingly similar for initial raw sequencing results and after being put through the same protocols, resulted in little usable molecular data. We discuss the successes and failures of using different pipelines and methods to maximize the products from these data and provide expectations for others who are looking to use DNA sequencing efforts on specimens that likely have degraded DNA.Life Science Identifier (Hydrablabes periops)urn:lsid:zoobank.org:pub:F2AA44 E2-D2EF-4747-972A-652C34C2C09D.
Metabolic Profiling at COVID-19 Onset Shows Disease Severity and Sex-Specific Dysregulation
Authors: Ceballos Francisco C. , Virseda-Berdices Ana , Resino Salvador , Ryan Pablo , Martínez-González Oscar , Peréz-García Felipe , Martin-Vicente María , Brochado-Kith Oscar , Blancas Rafael , Bartolome-Sánchez Sofía , Vidal-Alcántara Erick Joan , Albóniga-Díez Oihane Elena , Cuadros-González Juan , Blanca-López Natalia , Martínez Isidoro , Martinez-Acitores Ignacio Ramirez , Barbas Coral , Fernández-Rodríguez Amanda , Jiménez-Sousa María Ángeles 
Published: June 2022

Backgroundmetabolic changes through SARS-CoV-2 infection has been reported but not fully comprehended. This metabolic dysregulation affects multiple organs during COVID-19 and its early detection can be used as a prognosis marker of severity. Therefore, we aimed to characterize metabolic and cytokine profile at COVID-19 onset and its relationship with disease severity to identify metabolic profiles predicting disease progression.Material and Methodswe performed a retrospective cross-sectional study in 123 COVID-19 patients which were stratified as asymptomatic/mild, moderate and severe according to the highest COVID-19 severity status, and a group of healthy controls. We performed an untargeted plasma metabolic profiling (gas chromatography and capillary electrophoresis-mass spectrometry (GC and CE-MS)) and cytokine evaluation.ResultsAfter data filtering and identification we observed 105 metabolites dysregulated (66 GC-MS and 40 CE-MS) which shown different expression patterns for each COVID-19 severity status. These metabolites belonged to different metabolic pathways including amino acid, energy, and nitrogen metabolism among others. Severity-specific metabolic dysregulation was observed, as an increased transformation of L-tryptophan into L-kynurenine. Thus, metabolic profiling at hospital admission differentiate between severe and moderate patients in the later phase of worse evolution. Several plasma pro-inflammatory biomarkers showed significant correlation with deregulated metabolites, specially with L-kynurenine and L-tryptophan. Finally, we describe a strong sex-related dysregulation of metabolites, cytokines and chemokines between severe and moderate patients. In conclusion, metabolic profiling of COVID-19 patients at disease onset is a powerful tool to unravel the SARS-CoV-2 molecular pathogenesis.ConclusionsThis technique makes it possible to identify metabolic phenoconversion that predicts disease progression and explains the pronounced pathogenesis differences between sexes.
Tregs in Autoimmunity: Insights Into Intrinsic Brake Mechanism Driving Pathogenesis and Immune Homeostasis
Authors: Bednar Kyle J. , Lee Jee Ho , Ort Tatiana 
Published: June 2022

CD4+CD25highFoxp3+ regulatory T-cells (Tregs) are functionally characterized for their ability to suppress the activation of multiple immune cell types and are indispensable for maintaining immune homeostasis and tolerance. Disruption of this intrinsic brake system assessed by loss of suppressive capacity, cell numbers, and Foxp3 expression, leads to uncontrolled immune responses and tissue damage. The conversion of Tregs to a pathogenic pro-inflammatory phenotype is widely observed in immune mediated diseases. However, the molecular mechanisms that underpin the control of Treg stability and suppressive capacity are incompletely understood. This review summarizes the concepts of Treg cell stability and Treg cell plasticity highlighting underlying mechanisms including translational and epigenetic regulators that may enable translation to new therapeutic strategies. Our enhanced understanding of molecular mechanism controlling Tregs will have important implications into immune homeostasis and therapeutic potential for the treatment of immune-mediated diseases.
Global Synthesis of Air-Sea CO2 Transfer Velocity Estimates From Ship-Based Eddy Covariance Measurements
Authors: Yang Mingxi , Bell Thomas G. , Bidlot Jean-Raymond , Blomquist Byron W. , Butterworth Brian J. , Dong Yuanxu , Fairall Christopher W. , Landwehr Sebastian , Marandino Christa A. , Miller Scott D. , Saltzman Eric S. , Zavarsky Alexander 
Published: June 2022

The air-sea gas transfer velocity (K660) is typically assessed as a function of the 10-m neutral wind speed (U10n), but there remains substantial uncertainty in this relationship. HereK660of CO2derived with the eddy covariance (EC) technique from eight datasets (11 research cruises) are reevaluated with consistent consideration of solubility and Schmidt number and inclusion of the ocean cool skin effect.K660shows an approximately linear dependence with the friction velocity (u*) in moderate winds, with an overall relative standard deviation (relative standard error) of about 20% (7%). The largest relative uncertainty inK660occurs at low wind speeds, while the largest absolute uncertainty inK660occurs at high wind speeds. There is an apparent regional variation in the steepness of theK660-u*relationships: North Atlantic ≥ Southern Ocean &gt; other regions (Arctic, Tropics). Accounting for sea state helps to collapse some of this regional variability inK660using the wave Reynolds number in very large seas and the mean squared slope of the waves in small to moderate seas. The grand average of EC-derivedK660(−1.47 + 76.67u*+ 20.48u*2 or 0.36 + 1.203U10n+ 0.167U10n2)is similar at moderate to high winds to widely used dual tracer-basedK660parametrization, but consistently exceeds the dual tracer estimate in low winds, possibly in part due to the chemical enhancement in air-sea CO2exchange. Combining the grand average of EC-derivedK660with the global distribution of wind speed yields a global average transfer velocity that is comparable with the global radiocarbon (14C) disequilibrium, but is ~20% higher than what is implied by dual tracer parametrizations. This analysis suggests that CO2fluxes computed using aU10n2dependence with zero intercept (e.g., dual tracer) are likely underestimated at relatively low wind speeds.
The Qigong of Prolong Life With Nine Turn Method Relieve Fatigue, Sleep, Anxiety and Depression in Patients With Chronic Fatigue Syndrome: A Randomized Controlled Clinical Study
Authors: Xie Fangfang , You Yanli , Guan Chong , Xu Jiatuo , Yao Fei 
Published: June 2022

BackgroundChronic fatigue syndrome (CFS) is a complex disease of unknown etiology and mechanism. The purpose of this study was to investigate the effect of Prolong Life with Nine Turn Method (PLWNT) Qigong exercise on CFS focusing on fatigue, sleep quality, depression, and anxiety.MethodsA total of 90 participants diagnosed with CFS were randomly assigned into two parallel groups: PLWNT and cognitive behavioral therapy (CBT). The participants in the PLWNT or CBT group participated in qigong exercise or cognitive behavior education program, respectively, once a week in-person and were supervised online during the remaining 6 days at home, over 12 consecutive weeks. The primary outcome was fatigue (Multi-dimensional Fatigue Inventory 20 [MFI-20]), and secondary outcomes were sleep quality (Pittsburgh Sleep Quality Index [PSQI]), anxiety, depression (Hospital Anxiety and Depression Scale [HADS]), and changes in the Neuropeptide Y (NPY) of peripheral blood.ResultsThe within-group comparisons of the PLWNT and CBT groups revealed significant improvement in both groups in MFI-20, PSQI, and HADS scores (P &lt; 0.05). No significant difference were found between the PLWNT and CBT groups, even though the effective rate of the PLWNT group was 62.22%, which is slightly than 50.00% of the CBT group. The fatigue scores in the PLWNT group were positively correlated with sleep degree (r = 0.315) and anxiety degree (r = 0.333), only anxiety degree (r = 0.332) was found to be positively correlated with fatigue in the CBT group. The analysis of peripheral blood showed that NPY decreased after PLWNT intervention but increased significantly in the CBT.ConclusionThe PLWNT qigong exercise has potential to be an effective rehabilitation method for CFS symptoms including fatigue, sleep disturbance, anxiety, and depression. Future studies should expand study sample size for in-depth investigation to determine the optimal frequency and intensity of PLWNT qigong intervention in CFS patients. The study was registered in the ClinicalTrials.gov database on April 12, 2018, with registration number NCT03496961.
Prolactin Inducible Protein, but Not Prolactin, Is Present in Human Tears, Is Involved in Tear Film Quality, and Influences Evaporative Dry Eye Disease
Authors: Jüngert Katharina , Paulsen Friedrich , Jacobi Christina , Horwath-Winter Jutta , Garreis Fabian 
Published: June 2022

PurposeDecreased production of the aqueous component of the tear film is an important cause of the development of dry eye disease (DED). Tear production is influenced by hormones and hormone-like factors. Prolactin (PLR), a multifunctional pituitary gland hormone, is regularly present in the lacrimal gland of rats and rabbits. In humans, serum PLR concentration correlates with tear quality. To gain deeper insights of possible effects of PRL, prolactin receptor (PRLR) and prolactin inducible protein (PIP), we analyzed the three proteins in the human lacrimal apparatus and in reflex tears of healthy volunteers as well as patients suffering from DED.MethodsGene expression of PRLR and PIP was analyzed by RT-PCR in cadaveric human lacrimal gland and ocular surface tissues, immortalized human corneal epithelial cells (HCE and hTEPI) and human Meibomian gland epithelial cells (HMGECs). At the protein level, the expression and localization of PRL, PRLR and PIP in formalin-fixed paraffin sections of the lacrimal apparatus were studied by immunohistochemistry. In addition, tear fluid from DED patients and healthy volunteers was analyzed by ELISA to determine the concentration of PRL and PIP.ResultsRT-PCR analyses revealed gene expression of PRLR and PIP in human tissue samples of cornea, lacrimal glands, and eyelids, whereas only PIP, but not PRLR, was detectable in immortalized corneal epithelial cells. Immunohistochemistry revealed for the first time the expression and localization of PRL, PRLR, and PIP in human tissues of the lacrimal apparatus and at the ocular surface. PRL and PRLR were detectable in corneal epithelium, lacrimal glands, and Meibomian glands. Reflex tears from DED patients revealed significantly increased PIP concentrations, whereas PRL was undetectable in tears of DED patients and healthy volunteers.ConclusionPRL, PRLR, and PIP are found in the lacrimal apparatus and on the ocular surface. PIP, but not PRL, is present in human tears and appears to be involved in the physiology of tear film quality. Our clinical data revealed that PIP may affect tear quality, but further functional analyses are needed to fully elucidate the effects of PRL and PIP-associated factors in tear secretion as well as in the connection of DED.