bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metal ion scavenging activity of elastin-like peptide analogues containing a cadmium ion binding sequence
Authors: Sumiyoshi Shogo , Suyama Keitaro , Tatsubo Daiki , Tanaka Naoki , Tomohara Keisuke , Taniguchi Suguru , Maeda Iori , Nose Takeru 
Published: February 2022

AbstractThe development of simple and safe methods for recovering environmental pollutants, such as heavy metals, is needed for sustainable environmental management. Short elastin-like peptide (ELP) analogues conjugated with metal chelating agents are considered to be useful as metal sequestering agents as they are readily produced, environment friendly, and the metal binding domain can be selected based on any target metal of interest. Due to the temperature dependent self-assembly of ELP, the peptide-based sequestering agents can be transformed from the solution state into the particles that chelate metal ions, which can then be collected as precipitates. In this study, we developed a peptide-based sequestering agent, AADAAC-(FPGVG)4, by introducing the metal-binding sequence AADAAC on the N-terminus of a short ELP, (FPGVG)4. In turbidity measurements, AADAAC-(FPGVG)4 revealed strong self-assembling ability in the presence of metal ions such as Cd2+ and Zn2+. The results from colorimetric analysis indicated that AADAAC-(FPGVG)4 could capture Cd2+ and Zn2+. Furthermore, AADAAC-(FPGVG)4 that bound to metal ions could be readily recycled by treatment with acidic solution without compromising its metal binding affinity. The present study indicates that the fusion of the metal-binding sequence and ELP is a useful and powerful strategy to develop cost-effective heavy metal scavenging agents with low environmental impacts.
Gas fields and large shallow seismogenic reverse faults are anticorrelated
Authors: Valensise G. , Donda F. , Tamaro A. , Rosset G. , Parolai S. 
Published: February 2022

AbstractWe investigated the spatial relationships among 18 known seismogenic faults and 1651 wells drilled for gas exploitation in the main hydrocarbon province of northern-central Italy, a unique dataset worldwide. We adopted a GIS approach and a robust statistical technique, and found a significant anticorrelation between the location of productive wells and of the considered seismogenic faults, which are often overlain or encircled by unproductive wells. Our observations suggest that (a) earthquake ruptures encompassing much of the upper crust may cause gas to be lost to the atmosphere over geological time, and that (b) reservoirs underlain by smaller or aseismic faults are more likely to be intact. These findings, which are of inherently global relevance, have crucial implications for future hydrocarbon exploitation, for assessing the seismic–aseismic behaviour of large reverse faults, and for the public acceptance of underground energy and CO2 storage facilities—a pillar of future low carbon energy systems—in tectonically active areas.
Impact of long-term dietary habits on the human gut resistome in the Dutch population
Authors: Stege Paul B. , Hordijk Joost , Shetty Sudarshan A. , Visser Michael , Viveen Marco C. , Rogers Malbert R. C. , Gijsbers Esther , Dierikx Cindy M. , van der Plaats Rozemarijn Q. J. , van Duijkeren Engeline , Franz Eelco , Willems Rob J. L. , Fuentes Susana , Paganelli Fernanda L. 
Published: February 2022

AbstractThe human gut microbiome plays a central role in health and disease. Environmental factors, such as lifestyle and diet, are known to shape the gut microbiome as well as the reservoir of resistance genes that these microbes harbour; the resistome. In this study we assessed whether long-term dietary habits within a single geographical region (the Netherlands) impact the human gut resistome. Faecal samples from Dutch omnivores, pescatarians, vegetarians and vegans were analysed by metagenomic shotgun sequencing (MSS) (n = 149) and resistome capture sequencing approach (ResCap) (n = 64). Among all diet groups, 119 and 145 unique antibiotic resistance genes (ARGs) were detected by MSS or ResCap, respectively. Five or fifteen ARGs were shared between all diet groups, based on MSS and ResCap, respectively. The total number of detected ARGs by MSS or ResCap was not significantly different between the groups. MSS also revealed that vegans have a distinct microbiome composition, compared to other diet groups. Vegans had a lower abundance of Streptococcus thermophilus and Lactococcus lactis compared to pescatarians and a lower abundance of S. thermophilus when compared to omnivores. In summary, our study showed that long-term dietary habits are not associated with a specific resistome signature.
Ericaceous vegetation of the Bale Mountains of Ethiopia will prevail in the face of climate change
Authors: Kidane Yohannes O. , Hoffmann Samuel , Jaeschke Anja , Beloiu Mirela , Beierkuhnlein Carl 
Published: February 2022

AbstractClimate change impacts the structure, functioning, and distribution of species and ecosystems. It will shift ecosystem boundaries, potentially affecting vulnerable ecosystems, such as tropical Africa's high mountain ecosystems, i.e., afroalpine ecosystems, and their highly susceptible uniquely adapted species. However, ecosystems along these mountains are not expected to respond similarly to the change. The ericaceous woody vegetation, located between the low-elevation broadleaf forests and high-elevation afroalpine vegetation, are anticipated to be affected differently. We hypothesize that projected climate change will result in an upward expansion and increasing dominance of ericaceous vegetation, which will negatively impact the endemic rich afroalpine ecosystems of the extensive Sanetti plateau. Hence, we modeled the impact of future climate change on the distribution of ericaceous vegetation and discussed its effect on bordering ecosystems in the Bale Mountains. We applied four familiar correlative modeling approaches: bioclim, domain, generalized linear methods, and support vector machines. We used WorldClim’s bioclimatic variables as environmental predictors and two representative concentration pathways (RCPs) of the IPCC Fifth Assessment Report climate change scenarios, namely RCP4.5 and RCP8.5 for future climate projection. The results indicate increased ericaceous vegetation cover on the midaltitude of northwestern and northern parts of the massif, and the Sanetti plateau. We observed upward range expansion and increase of close ericaceous vegetation in midaltitudes, while receding from the lower range across the massif. Moreover, the current ericaceous vegetation range correlates to the temperature and precipitation trends, reaffirming the critical role of temperature and precipitation in determining species distributions along elevational gradients. The results indicate the high likelihood of considerable changes in this biodiversity hotspot in Eastern Africa.
Imaging of clinically silent rectoprostatic hematoma in MRI guided in-bore prostate biopsy
Authors: Garmer Marietta , Hoffmann Christin , Grönemeyer Dietrich , Wagener Birgit , Kamper Lars , Haage Patrick 
Published: February 2022

AbstractMR imaging provides awareness for rectoprostatic hematomas as a complication in prostate biopsy. We evaluated the frequency and size of clinically silent bleeding after in-bore MRI-guided prostate biopsy according to documentation in MRI. From 2007 until 2020 in-bore MRI-guided prostate biopsy was performed in 283 consecutive patients with suspected prostate cancer. Interventional image documentation was reviewed for rectoprostatic hematomas and rectal blood collections in this retrospective observational single-center study. Correlation to patient characteristics was analyzed using a multivariable logistic regression model. 283 consecutive patients with a mean age of 66 ± 8 years were included. We diagnosed bleeding complications in 41 (14.5%) of the patients. Significant rectoprostatic hematomas were found in 24 patients. Intra-rectal blood collections were observed in 16 patients and one patient showed bleeding in the urinary bladder. The volume of rectoprostatic hematomas was determined with a median of 7.5 ml (range 2–40 ml, IQR 11.25). We found no correlation between the presence of a rectoprostatic hematoma and malignant findings, patient position in biopsy, number of cores, age, prostate volume nor PSA density (p > 0.05). Rectoprostatic hematomas and rectal blood collections are rare complications after in-bore MR-guided prostate biopsy. MR imaging provides benefits not only for lesion detection in prostate biopsy but also for the control of bleeding complications, which can be overlooked in standard TRUS biopsy. Their significance in pain, erectile dysfunction, and urinary retention remains to be investigated.
Altered mechanotransduction in adolescent idiopathic scoliosis osteoblasts: an exploratory in vitro study
Authors: Oliazadeh Niaz , Gorman Kristen F. , Elbakry Mohamed , Moreau Alain 
Published: February 2022

AbstractAdolescent idiopathic scoliosis (AIS) is the most prevalent pediatric spinal deformity. We previously demonstrated elongated cilia and an altered molecular mechanosensory response in AIS osteoblasts. The purpose of this exploratory study was to characterize the mechanosensory defect occurring in AIS osteoblasts. We found that cilia length dynamics in response to flow significantly differ in AIS osteoblasts compared to control cells. In addition, strain-induced rearrangement of actin filaments was compromised resulting in a failure of AIS osteoblasts to position or elongate in function of the bidirectional-applied flow. Contrary to control osteoblasts, fluid flow had an inhibitory effect on AIS cell migration. Moreover, flow induced an increase in secreted VEGF-A and PGE2 in control but not AIS cells. Collectively our data demonstrated that in addition to the observed primary cilium defects, there are cytoskeletal abnormalities correlated to impaired mechanotransduction in AIS. Thus, we propose that the AIS etiology could be a result of generalized defects in cellular mechanotransduction given that an adolescent growing spine is under constant stimulation for growth and bone remodeling in response to applied mechanical forces. Recognition of an altered mechanotransduction as part of the AIS pathomechanism must be considered in the conception and development of more effective bracing treatments.
The transcription factor unc-130/FOXD3/4 contributes to the biphasic calcium response required to optimize avoidance behavior
Authors: Hori Sayaka , Mitani Shohei 
Published: February 2022

AbstractThe central neural network optimizes avoidance behavior depending on the nociceptive stimulation intensity and is essential for survival. How the property of hub neurons that enables the selection of behaviors is genetically defined is not well understood. We show that the transcription factor unc-130, a human FOXD3/4 ortholog, is required to optimize avoidance behavior depending on stimulus strength in Caenorhabditis elegans. unc-130 is necessary for both ON responses (calcium decreases) and OFF responses (calcium increases) in AIBs, central neurons of avoidance optimization. Ablation of predicted upstream inhibitory neurons reduces the frequency of turn behavior, suggesting that optimization needs both calcium responses. At the molecular level, unc-130 upregulates the expression of at least three genes: nca-2, a homolog of the vertebrate cation leak channel NALCN; glr-1, an AMPA-type glutamate receptor; and eat-4, a hypothetical L-glutamate transmembrane transporter in the central neurons of optimization. unc-130 shows more limited regulation in optimizing behavior than an atonal homolog lin-32, and unc-130 and lin-32 appear to act in parallel molecular pathways. Our findings suggest that unc-130 is required for the establishment of some AIB identities to optimize avoidance behavior.
Synthesis and characterization of novel combretastatin analogues of 1,1-diaryl vinyl sulfones, with antiproliferative potential via in-silico and in-vitro studies
Authors: Egharevba Godshelp O. , Kamal Ahmed , Dosumu Omotayo O. , Routhu Sunitha , Fadare Olatomide A. , Oguntoye Stephen O. , Njinga Stanislaus N. , Oluyori Abimbola P. 
Published: February 2022

AbstractNovel 1,1-diaryl vinyl-sulfones analogues of combretastatin CA-4 were synthesized via Suzuki–Miyaura coupling method and screened for in-vitro antiproliferative activity against four human cancer cell lines: MDA-MB 231(breast cancer), HeLa (cervical cancer), A549 (lung cancer), and IMR-32 (neuroblast cancer), along with a normal cell line HEK-293 (human embryonic kidney cell) by employing 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The compounds synthesised had better cytotoxicity against the A549 and IMR-32 cell lines compared to HeLa and MDA-MB-231 cell lines. The synthesized compounds also showed significant activity on MDA-MB-231 cancer cell line with IC50 of 9.85–23.94 µM, and on HeLa cancer cell line with IC50 of 8.39–11.70 µM relative to doxorubicin having IC50 values 0.89 and 1.68 µM respectively for MDA-MB-231 and HeLa cell lines. All the synthesized compounds were not toxic to the growth of normal cells, HEK-293. They appear to have a higher binding affinity for the target protein, tubulin, PDB ID = 5LYJ (beta chain), relative to the reference compounds, CA4 (− 7.1 kcal/mol) and doxorubicin (− 7.2 kcal/mol) except for 4E, 4M, 4N and 4O. The high binding affinity for beta-tubulin did not translate into enhanced cytotoxicity but the compounds (4G, 4I, 4J, 4M, 4N, and 4R, all having halogen substituents) that have a higher cell permeability (as predicted in-silico) demonstrated an optimum cytotoxicity against the tested cell lines in an almost uniform manner for all tested cell lines.
The in-silico study provided insight into the role that cell permeability plays in enhancing the cytotoxicity of this class of compounds and as potential antiproliferative agents.
Spatiotemporal sentiment variation analysis of geotagged COVID-19 tweets from India using a hybrid deep learning model
Authors: Kumar Vaibhav 
Published: February 2022

AbstractIndia is a hotspot of the COVID-19 crisis. During the first wave, several lockdowns (L) and gradual unlock (UL) phases were implemented by the government of India (GOI) to curb the virus spread. These phases witnessed many challenges and various day-to-day developments such as virus spread and resource management. Twitter, a social media platform, was extensively used by citizens to react to these events and related topics that varied temporally and geographically. Analyzing these variations can be a potent tool for informed decision-making. This paper attempts to capture these spatiotemporal variations of citizen reactions by predicting and analyzing the sentiments of geotagged tweets during L and UL phases. Various sentiment analysis based studies on the related subject have been done; however, its integration with location intelligence for decision making remains a research gap. The sentiments were predicted through a proposed hybrid Deep Learning (DL) model which leverages the strengths of BiLSTM and CNN model classes. The model was trained on a freely available Sentiment140 dataset and was tested over manually annotated COVID-19 related tweets from India. The model classified the tweets with high accuracy of around 90%, and analysis of geotagged tweets during L and UL phases reveal significant geographical variations. The findings as a decision support system can aid in analyzing citizen reactions toward the resources and events during an ongoing pandemic. The system can have various applications such as resource planning, crowd management, policy formulation, vaccination, prompt response, etc.
Molecular diagnosis of alveolar echinococcosis in patients based on frozen and formalin-fixed paraffin-embedded tissue samples
Authors: Knapp Jenny , Lallemand Séverine , Monnien Franck , Felix Sophie , Valmary-Degano Séverine , Courquet Sandra , Demonmerot Florent , Heyd Bruno , Turco Celia , Doussot Alexandre , Bourgeois Lucie , Bresson-Hadni Solange , Richou Carine , Millon Laurence 
Published: February 2022

Confirmed diagnosis of alveolar echinococcosis (AE) is based on pathological criteria and molecular evidence. This parasite-borne disease, caused by the cestode Echinococcus multilocularis, sparingly involves humans as a dead-end host. In humans, the parasite mainly colonizes the liver but can colonize any organ and cause atypical forms, often difficult to characterize clinically. Moreover, molecular methods may be suitable to make the diagnosis of AE in cases of atypical forms, extra-hepatic localizations, or immunosuppressed patients. The aim of this study was to determine the most relevant published PCR techniques, for diagnosis of AE in patients and adopt the best strategy for molecular diagnosis depending on the nature of the tested sample. In this study, we evaluated nine end-point PCR assays and one real-time PCR assay (qPCR), targeting mitochondrial genes, using a total of 89 frozen or formalin-fixed paraffin-embedded (FFPE) samples from either 48 AE or 9 cystic echinococcosis patients. Targeted fragment-genes ranged from 84 to 529 bp. Six PCR assays were able to amplify the DNA of 100% of the frozen AE-samples and for one PCR, 69.8% of the FFPE AE-samples. The 16S rrnL PCR (84 bp) was positive in PCR for 77% of the AE samples and in qPCR for 86.5%. The sensitivity of the PCR assays was higher for fresh samples and FFPE samples stored for less than 5 years. The qPCR assay further increased sensitivity for the tested samples, confirming the need for the development of an Echinococcus spp. qPCR to improve the molecular diagnosis of echinococcoses.
Keep that mask on: will Germans become more like East Asians?
Authors: Matschke Xenia , Rieger Marc Oliver 
Published: February 2022

AbstractWe study planned changes in protective routines after the COVID-19 pandemic: in a survey in Germany among >650 respondents, we find that the majority plans to use face masks in certain situations even after the end of the pandemic. We observe that this willingness is strongly related to the perception that there is something to be learned from East Asians’ handling of pandemics, even when controlling for perceived protection by wearing masks. Given strong empirical evidence that face masks help prevent the spread of respiratory diseases and given the considerable estimated health and economic costs of such diseases even pre-Corona, this would be a very positive side effect of the current crisis.
Simulation analysis and experimental research on the safety distance of UAV inspection on transmission lines
Authors: Mao Xiaopo , Xiang Bin , Liu Zhuang , Tu Suge 
Published: February 2022

In recent years, unmanned aerial vehicle (UAV) inspection has become one of the main means of daily operation and maintenance of overhead transmission lines. Since the key components of the UAV inspection system are composed of electronic components, it relies on data communication to realize data link transmission. In the actual operation process, the complex electromagnetic field environment has an obvious impact on its control and communication performance, especially when inspecting near the DC line. In this paper, combined with the research on the safety assurance technology of UAV inspection operations, the electromagnetic field simulation model of the UAV inspection operation on DC transmission lines is established. The electromagnetic field distribution around the wire is analyzed when the UAV is at different inspection distances, and the influence range and degree of the DC electromagnetic field on UAV inspection system are obtained. A true test platform for the inspection safety distance of the ±500 kV line UAV inspection was built, and the inspection safety distance test and research of the small rotary-wing UAV with a typical size, structure, and material was carried out. Through the test, when the current of the transmission line is 3 kA, the minimum safety distance of the UAV inspection operation of the ±500 kV DC transmission line straight tower is 3 m, and the distance is corrected in combination with the current. The research results of this paper can provide technical support for the inspection operation of small multi-rotor UAVs on ±500 kV transmission lines.
Sensor-based detection of parturition in beef cattle grazing in an extensive landscape: a case study using a commercial GNSS collar
Authors: Williams T. M. , Costa D. F. A. , Wilson C. S. , Chang A. , Manning J. , Swain D. , Trotter M. G. 
Published: February 2022

Context Neonate management remains a key issue in extensive beef production systems where producers are faced with substantial environmental and management challenges that limit their ability to monitor and manage livestock in a timely manner. Parturition is a critical event and can affect the calf health and survival, particularly in the perinatal period (up to 48 h after birth). As such, monitoring parturition using precision livestock technologies may provide producers with additional tools to manage their cattle and mitigate the impacts of neonatal mortality in extensive beef systems. Aims The purpose of this study was to determine whether data from a global navigation satellite system (GNSS) collar could be used to detect parturition events in extensively grazed beef cattle. Methods Forty-eight Bos taurus cows (583.5 kg body weight ± 9.25 s.e.m.) were allocated to a 28 ha paddock between 8 January 2021 and 6 March 2021 during the calving season. Thirty of the animals were fitted with GNSS-equipped collars (Smart Paddock, Vic., Australia) that captured data at 10 min intervals. Parturition events were recorded daily by visual observation. Collected data were used to calculate key predictive features related to calving behaviour. Derived features were compared and assessed for changes in the period surrounding parturition. Key results Increases were observed in distance to nearest neighbour and to herd aggregate features, and decreases were observed in paddock utilisation and distance travelled features in the lead-up to calving (P < 0.05). Furthermore, the number of animals within a 20 m radius decreased significantly (P < 0.05) in the lead up to parturition, supporting known isolation behaviours. Conclusions With further development of predictive algorithms, on-animal sensors may be valuable in the prediction of calving events in extensive beef production systems. Implications Remote management and monitoring with on-animal sensor technologies, such as GNSS collars and tags, will provide producers with an additional means of monitoring their animals, while overcoming many of the management challenges associated with extensive grazing operations.
Asturian and Asturian Spanish at the syntax-phonology interface
Authors: Villa-García Julio , Sánchez-Llana Hugo 
Published: February 2022

Abstract
A number of movement-related phenomena from Asturian as well as from the Spanish spoken in the Principality of Asturias are amenable to accounts where the Pronounce-Highest-Copy requirement in Phonetic Form can be overridden if convergence so demands. Therefore, the evidence adduced here supports the view that the requirements imposed by the Phonetic Form component may take precedence over purely syntactic requirements. In this connection, we argue for a novel analysis of the enclitic-proclitic contrast in Asturian and Asturian Spanish in finite and non-finite contexts, alongside extensions to other constructions, which add to the ample stock of phenomena supporting the rather successful Copy Theory of Movement.