bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visual detection of Shigella in milk by competitive annealing‐mediated isothermal amplification with paper‐based DNA extraction method
Authors: Chen Xu , Ma Yue , Miao Shuangyu 
Published: July 2022

SummaryShigella is the main cause of endemic diarrhoea in low‐income countries. Fast and accurate detection of this pathogen can effectively prevent the consumption of contaminated food and reduce the risk of diarrhoea outbreaks. Recently, a competitive annealing‐mediated isothermal amplification (CAMP) assay was proposed as a novel nucleic acid detection technology. In this study, we developed a visual CAMP assay based on the invasive plasmid antigen H (ipaH) gene for rapid detection of Shigella in milk. Without enrichment culture, the visual CAMP assay could reliably detect Shigella at 2.6 × 102 CFU mL−1 or higher contamination levels. With enrichment culture, even if the spiked level is 1 CFU mL−1, the visual CAMP assay could reliably detect Shigella from the milk sample. Furthermore, a simple paper‐based DNA extraction method was established. The efficiency of the paper‐based method was comparable to that of the commercial kit method, and the entire extraction procedure can be completed in 15 min. The visual CAMP assay combined with the paper‐based DNA extraction method showed higher sensitivity and specificity for Shigella on‐site detection in milk samples.
Impact of storage conditions on the composition and antioxidant activity of peanut skin phenolic‐based extract
Authors: Bodoira Romina , Rossi Yanina , Velez Alexis , Montenegro Mariana , Martínez Marcela , Ribotta Pablo , Maestri Damián 
Published: July 2022

SummaryWith a view to its potential application as a food antioxidant, this study evaluated the impact of storage conditions (ReTD: darkness, 4 °C; RoTD: darkness, 25 °C; RoTL: light, 25 °C) on the composition and antioxidant activity of a peanut skin phenolic‐based extract (PSE). Results showed that the composition and several selected antioxidant capacities of PSE were preserved when stored in ReTD. Under these conditions, most of the identified compounds did not show significant losses in concentration at the final storage time (12 months). When tested in a highly unsaturated lipid system, this extract also showed the same antioxidant efficacy as the original extract (t0). Meanwhile, reducing power and radical scavenging activities of PSE stored in darkness were virtually retained; irrespective of the temperature condition, the parameters analysed remained almost constant over all the storage time. Overall, the results highlight the importance of the dark condition as a key factor for the PSE's composition and antioxidant activity preservation. In conclusion, the research findings suggest simple and low‐cost conditions that guarantee the conservation of the PSE composition and the continuity of its antioxidant properties during prolonged storage.
Whole‐wheat bread enzymatically bioprocessed and added with green coffee infusion had improved volume and were sensory accepted when consumers were informed of the presence of healthy substances
Authors: de Almeida Suellen Silva , Brito‐Silva Lillyan , da Costa Gabriela Bouça Marques , Barreto Maysa Silva , Freire Denise Maria Guimarães , Cadena Rafael Silva , Monteiro Mariana , Perrone Daniel , Moura‐Nunes Nathália 
Published: July 2022

SummaryThis study aimed at investigating the effect of enzymatic bioprocessing and the addition of green coffee infusion on the nutritional, technological, and sensory characteristics of whole‐wheat bread as well as of information on sensory profile. The proposed strategies increased minerals and soluble fibre content. Green coffee addition increased phenolics content up to 58% and incorporated caffeine to its composition. Bioprocessing increased bread volume up to 18%. Both strategies modified breads' colour perception. In the blind CATA test, breads were grouped according to the employment of enzymatic bioprocessing, but not to the addition of green coffee infusion. Non‐bioprocessed samples were characterised with more favourable terms. The acceptance of bioprocessed breads was improved once information regarding the presence of healthy substances were given. Therefore, the use of both strategies was successful to improve technological and nutritional benefits when information was given to consumers, yielding a bread with promising market potential.
Analysis of physicochemical properties, fatty acid composition and antioxidant activity of seed oil extracted from 12 citrus materials
Authors: Liu Wei , Mao Xiaoxue , Zhou Zhiqin 
Published: July 2022

SummaryThe massive citrus seed waste provides a new approach to the shortage of edible oils in China. In this study, the physicochemical properties, fatty acid composition and antioxidant activity of 12 citrus seed oils from China were determined, aiming to provide a theoretical basis for the development and utilisation of citrus seed oil. Results show most of the citrus seeds had a high oil content (28.3%–41.4%), with 2.6% in Zhi (Poncirus trifoliata). The crude oil extracted from the seeds of Dahongpao (Citrus reticulata) and Tongnanningmeng (Citrus limon) could directly meet the ‘National Standard for Food Safety Vegetable Oil’ (GB 2716‐2018). With total phenolic content (TPC) (62.1–2086.8 μg GAE g−1 DW), total flavonoid content (TFC) (59.6–2275.3 μg RE g−1) and total carotenoid content (TCC) (37.4–896.6 μg g−1), seed oil of Zhi had the highest content of bioactive substances and the strongest antioxidant capacity. The content of unsaturated fatty acids in citrus seed oil ranged from 72.4% to 82.2%, and the SFA: MUFA: PUFA ratio was closer to the recommended intake ratio of 1:1:1 by the Chinese Nutrition Society than other vegetable oils, which have higher nutritional value. Therefore, citrus seed oil is a potentially valuable oil source for healthy edible oil.
Mass transfer kinetics study for improving the uniform quality of lactic acid marinated pork (longissimus dorsi muscle)
Authors: Liu Chongxin , Zhang Chunjiang 
Published: July 2022

SummaryThe aim of this work was to study the rule of the different concentrations on marination kinetics and mass transfer of lactic acid‐marinated pork (longissimus dorsi muscle) specimens. The marinades of lactic acid concentrations at 0.5%, 1.0%, 1.5%, 2.0%, and 2.5% (w/w) and the marination process was performed at 1, 2, 3, 4, and 5 h, respectively. The water holding capacity (WHC), water and lactic acid content of the pork specimens were measured. Mass transfer kinetic curves were fitted. Kinetic parameter values for changes in total pork mass, the content of water and lactic acid, and effective diffusion coefficient were calculated. The obtained results indicated that the values of kinetic parameters for the changes in total mass, the content of water and lactic acid, and WHC were all increased with lactic acid concentration and marination time raised. The prediction model of water and lactic acid mass transfer in pork had a good linear correlation with marination time. The apparent diffusion coefficient value was the largest and the diffusion rate was the fastest when the mass fraction of lactic acid in the marinade was 1.5%. This research could provide effective parameters for improving the efficiency and uniformly quality of lactic acid marinated meat products.
Incorporation of germinated mung bean flour with rice flour to enhance physical, nutritional and sensory quality of gluten‐free cookies
Authors: Yen Nguyen Thi Hoang , Huong Nguyen Thi Mai , Hoa Phan Ngoc , Van Hung Pham 
Published: July 2022

SummaryQuality of the cookies substituted with germinated mung bean flour (GMF) to rice flour (RF) to produce gluten‐free cookies were investigated in this study. The GMF was used to formulate with the RF to obtain the composite flours with different total protein contents of 6, 9, 12, 15, 18 and 21 (%, d.b). The increase in the substituted amounts of GMF contributed to the lower baking loss, diameter and spread ratio, but the higher thickness and hardness of the cookies as compared to the RF‐based cookie. The cookies with more GMF substitution expressed lower lightness (L*) values, but higher redness (a*) and yellowness (b*) values than the others. The cookies substituted with 60% of the GMF (60‐GMFC) exhibited similar baking loss, thickness, diameter, spread ratio and lightness values to the wheat flour (WF)‐based cookies. The total amounts of essential amino acids and the sum of histidine, lysine and methionine of the composite flour‐based cookies were 3.90% and 1.64%, significantly higher than those of the RF‐based cookie (1.93% and 0.66%, respectively) or the WF‐based cookie (1.70% and 0.58%, respectively). As a result, healthy gluten‐free cookies could be prepared using the RF with the substitution of the germinated mung bean flour.
Allogeneic haemopoietic cell transplant services in Australia and New Zealand in the first year of the COVID‐19 pandemic: a report from Australia and New Zealand transplant and cellular therapies
Authors: Othman Jad , Aarons Donna , Bajel Ashish , Butler Jason , Doocey Richard , O'Brien Tracey , Purtill Duncan , Smith Lisa , Wilcox Leonie , Hamad Nada 
Published: July 2022

AbstractBackgroundThe COVID‐19 pandemic has caused major disruption to health systems, with allogeneic haemopoietic cell transplant (alloHCT) services a particularly vulnerable area. Ongoing provision of alloHCT has required dynamic responses at national and local levels. In Australia and New Zealand (ANZ), a high reliance on unrelated donors from overseas registries has posed an additional challenge.AimsTo describe the impact of COVID‐19 on alloHCT services in ANZ in the first year of the pandemic.MethodsData from the national alloHCT patient and unrelated donor registries were extracted for a 2‐year time frame. Comparisons were made between a pre‐pandemic period of 1 March 2019 to 29 February 2020 and the corresponding dates during the pandemic, 1 March 2020 to 28 February 2021.ResultsThere was a 13% decrease in the number of allogeneic transplants, a reversal of steady increases in previous years, with the largest decrease in unrelated donor transplants. Local donors supplied a greater proportion of unrelated stem cell products. With a switch to universal cryopreservation, the time from request of a product to infusion increased by a median of 25.5 days for overseas products and 14 days for local products. There was a significant increase in the number of products collected but not used.ConclusionsA strong public health response and coordinated transplant community activities allowed for safe provision of alloHCT in ANZ; however, our data suggest that the timely delivery of allogeneic transplants was affected by the COVID‐19 pandemic. Continued dedicated efforts are required to minimise further impacts.
Violation of non‐adjacent rule dependencies elicits greater attention to a talker's mouth in 15‐month‐old infants
Authors: Birulés Joan , Martinez‐Alvarez Anna , Lewkowicz David J. , de Diego‐Balaguer Ruth , Pons Ferran 
Published: July 2022

AbstractInfants start tracking auditory‐only non‐adjacent dependencies (NAD) between 15 and 18 months of age. Given that audiovisual speech, normally available in a talker's mouth, is perceptually more salient than auditory speech and that it facilitates speech processing and language acquisition, we investigated whether 15‐month‐old infants' NAD learning is modulated by attention to a talker's mouth. Infants performed an audiovisual NAD learning task while we recorded their selective attention to the eyes, mouth, and face of an actress while she spoke an artificial language that followed an AXB structure (tis‐X‐bun; nal‐X‐gor) during familiarization. At test, the actress spoke the same language (grammatical trials; tis‐X‐bun; nal‐X‐gor) or a novel one that violated the AXB structure (ungrammatical trials; tis‐X‐gor; nal‐X‐bun). Overall, total duration of looking did not differ during the familiar and novel test trials but the time‐course of selective attention to the talker's face and mouth revealed that the novel trials maintained infants' attention to the face more than did the familiar trials. Crucially, attention to the mouth increased during the novel test trials while it did not change during the familiar test trials. These results indicate that the multisensory redundancy of audiovisual speech facilitates infants' discrimination of non‐adjacent dependencies.
Recommendations to support the mental wellbeing of nurses and midwives in the United Kingdom: A Delphi study
Authors: Teoh Kevin Rui‐Han , Kinman Gail , Harriss Anne , Robus Christopher 
Published: July 2022

AbstractAimTo use the Delphi technique to identify and prioritize recommendations for research and practice to improve the mental wellbeing of nurses and midwives in the United Kingdom (UK).BackgroundAlthough there is evidence that self‐reported mental wellbeing among nurses and midwives in the UK is poor, interventions have not adequately considered the wider context in which they work. The wide range of individual, organizational, occupational and wider sector‐level factors that can influence wellbeing requires the involvement of different stakeholders to identify the most pressing actions required.DesignA three‐round Delphi technique was conducted in 2019.MethodsIn the first round, 16 subject matter experts generated, reviewed and discussed recommendations from a review of the research evidence with potential to support the mental wellbeing of nurses. A second group with 23 stakeholder representatives then rated and provided feedback on the developed recommendations through two additional rounds. Recommendations that received an ‘essential’ or ‘important’ rating from at least 80% of participants were retained and prioritized.ResultsIn total, 45 recommendations met the consensus agreement and were retained. More than half (57%) involved action at the organizational level, 27% to public policy and 13% to research. Only one recommendation is related to the individual. Collectively, these recommendations highlight the importance of taking direct action to tackle poor mental wellbeing among the workforce and initiating change at the policy and organizational level.ConclusionOur findings emphasize the need to take a systemic approach to improving the mental health of nurses and midwives in the UK with input from different stakeholders. There is a clear consensus that action is needed at the organization and policy levels, rather than at the individual level as is current practice.ImpactThis study provides a framework, alongside a set of practical recommendations, that provides a starting point for different stakeholders to understand, address and support the mental wellbeing of nurses and midwives. Although UK‐focused, it has relevance to healthcare workforces internationally.
A randomized multi‐centre study on the effectiveness of non‐surgical periodontal therapy in general practice
Authors: Tomasi Cristiano , Liss Anna , Welander Maria , Alian Anna Ydenius , Abrahamsson Kajsa H. , Wennström Jan L. 
Published: July 2022

AbstractAimTo evaluate the effectiveness of two non‐surgical treatment protocols for periodontitis patients in general dental practice.Materials and MethodsNinety‐five dental hygienists (59 dental clinics) were randomly assigned to one of two treatment protocols: (i) establishment of adequate self‐performed oral hygiene prior to a single session of ultrasonic instrumentation (guided periodontal infection control [GPIC]) or (ii) conventional non‐surgical therapy (CNST) including patient education and scaling and root planing integrated in multiple sessions. Residual pockets at 3 months were retreated in both groups. The primary outcome was pocket closure (probing pocket depth ≤ 4 mm) at 6 months. Multilevel models were utilized.ResultsBased on data from 615 patients, no significant differences with regard to clinical outcomes were observed between treatment protocols. Treatment‐related costs (i.e., chair time, number of sessions) were significantly lower for GPIC than CNST. Smoking and age significantly affected treatment outcomes.ConclusionsNo significant differences between the two approaches were observed in regard to clinical outcomes. GPIC was more time‐effective. Patient education should include information on the detrimental effects of smoking. ClinicalTrials.gov (NCT02168621).
Mother's and children's ADHD genetic risk, household chaos and children's ADHD symptoms: A gene–environment correlation study
Authors: Agnew‐Blais Jessica C. , Wertz Jasmin , Arseneault Louise , Belsky Daniel W. , Danese Andrea , Pingault Jean‐Baptiste , Polanczyk Guilherme V. , Sugden Karen , Williams Benjamin , Moffitt Terrie E. 
Published: July 2022

BackgroundChaotic home environments may contribute to children's attention‐deficit hyperactivity disorder (ADHD) symptoms. However, ADHD genetic risk may also influence household chaos. This study investigated whether children in chaotic households had more ADHD symptoms, if mothers and children with higher ADHD genetic risk lived in more chaotic households, and the joint association of genetic risk and household chaos on the longitudinal course of ADHD symptoms across childhood.MethodsParticipants were mothers and children from the Environmental Risk (E‐Risk) Longitudinal Twin Study, a UK population‐representative birth cohort of 2,232 twins. Children's ADHD symptoms were assessed at ages 5, 7, 10 and 12 years. Household chaos was rated by research workers at ages 7, 10 and 12, and by mother's and twin's self‐report at age 12. Genome‐wide ADHD polygenic risk scores (PRS) were calculated for mothers (n = 880) and twins (n = 1,999); of these, n = 871 mothers and n = 1,925 children had information on children's ADHD and household chaos.ResultsChildren in more chaotic households had higher ADHD symptoms. Mothers and children with higher ADHD PRS lived in more chaotic households. Children's ADHD PRS was associated with household chaos over and above mother's PRS, suggesting evocative gene–environment correlation. Children in more chaotic households had higher baseline ADHD symptoms and a slower rate of decline in symptoms. However, sensitivity analyses estimated that gene–environment correlation accounted for a large proportion of the association of household chaos on ADHD symptoms.ConclusionsChildren's ADHD genetic risk was independently associated with higher levels of household chaos, emphasising the active role of children in shaping their home environment. Our findings suggest that household chaos partly reflects children's genetic risk for ADHD, calling into question whether household chaos directly influences children's core ADHD symptoms. Our findings highlight the importance of considering parent and child genetic risk in relation to apparent environmental exposures.
Combined effects of insecticide and IGP on native and invasive ladybeetles in apple orchard
Authors: De Donder Alice , Cormier Daniel , Rodríguez García Marcela Andrea , Lucas Eric 
Published: July 2022

AbstractThe Asian ladybeetle Harmonia axyridis Pallas is an invasive species commonly found in Canadian apple orchards. It threatens native ladybeetles through exploitative competition and intraguild predation (IGP), including the 12 spotted ladybeetle Coleomegilla maculata DeGeer (Gagnon, Heimpel and Brodeur, 2011). Both species are exposed to novaluron, a reduced risk insecticide used against the codling moth Cydia pomonella (L.), the primary pest in apple orchards. Previous research showed that novaluron is highly toxic for the invasive H. axyridis, but it is less toxic for the indigenous C. maculata. Novaluron reduces the adaptive value of IGP for H. axyridis but not for C. maculata. However, there is a lack of knowledge on the combined effect of novaluron and IGP outside laboratory conditions. This study explores under semi‐field conditions the effect of novaluron residues and IGP on the survival of H. axyridis and C. maculata larvae. IGP was assessed considering third instar larvae as predators. Results showed that novaluron was toxic for first instar larvae of both species after 10 days of exposure. When H. axyridis was the intraguild predator, novaluron increased the survival of the intraguild preys. Novaluron did not change the outcome of the IGP when C. maculata was the intraguild predator. These results indicate that novaluron can change the competition outcome between the invasive H. axyridis and a native ladybeetle species. This is the first demonstration that intraguild predation and competition may be greatly altered in the presence of pesticides.
Temperature effects on teleost immunity in the light of climate change
Authors: Scharsack Jörn Peter , Franke Frederik 
Published: July 2022

AbstractTemperature is an important environmental modulator of teleost immune activity. Susceptibility of teleosts to temperature variation depends on the species‐specific adaptive temperature range, and the activity of the teleost immune system is generally temperature‐dependent. Similar to many physiological and metabolic traits of ectotherms, temperature modulates the activity of immune traits. At low temperatures, acquired immunity of many teleost species is down‐modulated, and their immuno‐competence mainly depends on innate immunity. At intermediate temperatures, both innate and acquired immunity are fully active and provide optimal protection, including long‐lasting immunological memory. When temperatures increase and reach the upper permissive range, teleost immunity is compromised. Moreover, temperature shifts may have negative effects on teleost immune functions, in particular if shifts occur rapidly with high amplitudes. On the contrary, short‐term temperature increase may help teleost immunity to fight against pathogens transiently. A major challenge to teleosts therefore is to maintain immuno‐competence throughout the temperature range they are exposed to. Climate change coincides with rising temperatures, and more frequent and more extreme temperature shifts. Both are likely to influence the immuno‐competence of teleosts. Nonetheless, teleosts exist in habitats that differ substantially in temperature, ranging from below zero in the Arctic's to above 40°C in warm springs, illustrating their enormous potential to adapt to different temperature regimes. The present review seeks to discuss how changes in temperature variation, induced by climate change, might influence teleost immunity.