bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of Domestic Violence and its associated factors among ever-married reproductive age Women in Cameroon: A cross-sectional survey
Authors: Mulat Bezawit , Tsegaw Menen , Chilot Dagmawi , Shitu Kegnie 
Published: March 2022

Abstract
BackgroundDomestic violence (DV) against women is a global issue that affects women in all countries and is a significant contributor to their poor health. Women who have experienced DV, in particular, suffer from the gynecological, nervous system, and stress-related issues. Despite such devastating implications, there is a paucity of data on the prevalence of DV and its associated factors among married reproductive-age women in Cameroon.MethodA community-based crossectional demographic and health survey was conducted in Cameroon in 2018. The participants were chosen using a two-stage stratified selection procedure. To find factors linked to domestic violence, we employed multivariable logistic regression analysis. Statistical significance was determined using a p-value of less than 0.05 and a 95 % confidence range.ResultA total of 4,903 ever-married women were included in the study. The mean age of the participants was 31.3 (± 8.4) years with an age range of 15-49 years. The prevalence of domestic violence among ever-married Cameroonian women was 30.5% with 95%CI (29.3%, 31.8%). womens’ educational attainment (higher education) (AOR=0.57, 95% CI (0.36, 1.04)), Husband education (husbands who attended primary educational level (AOR=1.29, 95% CI (1.00, 1.66). Plus to the above factors women whose husband alcohol (AOR= 3.00, 95% CI (2.56, 3.52)), and the region where the women reside in (center without Yaoundé (AOR= 2.54, 95% CI (1.79, 3.60), west (AOR=1.54, 95% CI (1.09, 2.19), South (AOR=1.99, 95% CI (1.18, 2.50), and Yaoundé (AOR=1.72. 95% CI(1.18,2.50) were significantly associated with womens’ domestic violence by their partner in the past 12 months.ConclusionThe present study revealed that the prevalence of domestic violence among ever-married women in Cameroon is high. Womens’ education, husband education, husband alcohol drinking, and the region where the women reside in the country are factors significantly associated with domestic violence.
Mycobacterium tuberculosis specific antigen-stimulated CD27-CD38+IFN-γ+CD4+ T cells for active tuberculosis diagnosis
Authors: Su Bo , fang Yong , Wang Na , Tang Liang , Yang Xiaojun , Tang Yuan , Li Lin , Wu Wenfei , Sha Wei 
Published: March 2022

Abstract
Background: Traditional bacteriological tests are time-consuming and of low sensitivity for the diagnosis of active tuberculosis (ATB). The purpose of this study is to investigate the accuracy of CD27-CD38+IFN-γ+CD4+/CD4+ for active tuberculosis (ATB) diagnosis. Methods: This prospective diagnostic accuracy study was conducted at Shanghai Pulmonary Hospital between January 2019 and December 2021. Patients with ATB, non-tuberculosis mycobacterium infection (NTM), latent tuberculosis infection (LTBI), other respiratory diseases (OD), and healthy individuals (HC) were enrolled. The accuracy of CD27-CD38+IFN-γ+CD4+/CD4+ and other phenotypic markers for ATB diagnosis was assessed.Results: A total of 376 patients (237 ATB, 38 LTBI, 8 NTM, 50 OD, and 43 HC) were enrolled. The ratios of CD4+IFN-γ+CD27- and CD4+IFN-γ+CD27-CD38+ profiles in CD4+IFN-γ+ cells and the ratios of CD4+IFN-γ+CD38+, CD4+IFN-γ+CD27-, and CD4+IFN-γ+CD38+CD27- profiles in CD4+ cells in the ATB group were significantly higher than in the other groups. The area under the curve (AUC) of CD27-CD38+IFN-γ+CD4+/CD4+ for the diagnosis of ATB was the highest, with a value of 0.890. With the optimal cutoff value of 1.34´10-4, the sensitivity and specificity of CD27-CD38+IFN-γ+CD4+/CD4+ for ATB diagnosis was 0.869 and 0.849, respectively. Conclusion: CD27-CD38+IFN-γ+CD4+/CD4+ might be an effective biomarker for active tuberculosis diagnosis.
An inflammation-related prognostic model risk score predicts the prognosis of Clear cell renal cell carcinoma
Authors: Cheng Hui , Zhang Yuxin , Sheng Jiajie , Chen Weijian , Chen Binhai 
Published: March 2022

Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors of the urinary system, and the prognosis of patients with advanced stage is really poor. Existing evidence suggests that inflammation and inflammation-related genes play complex roles in different tumors, but their role in ccRCC has rarely been studied as a primary research object. Methods: we used The Cancer Genome Atlas (TCGA) database to established a prognostic model risk score for ccRCC and inflammation-related genes and verified its predictive effect on the prognosis of ccRCC.Result: We screened 10 inflammatory differentially expressed genes (DEGs) with independent prognostic value for ccRCC and constructed a prognostic model risk score: (-0.0153×APLNR) + (-0.0073×BTG2) +0. 0225×CSF1+ (-0.0107×CX3CL1) +0. 1888×GABBR1+0. 1528×HAS2+0. 0088×ICAM1+0. 3952×P2RY2+ (-0.0442×SPHK1) +0. 0006×TIMP1. The survival analysis showed that ccRCC with a higher risk score implies shorter survival and worse prognosis. Then we used univariate and multivariate Cox regression analysis and Receiver Operating Characteristic (ROC) curve to confirm that the risk score has a good and stable independent prognostic value. and performed an internal validation of the risk score. Gene set enrichment analysis (GSEA) showed that high risk groups were involved in many pathways related to the occurrence and development of tumors. we also found that the expression levels of immune checkpoints including PD-1, CTLA-4, LAG3, TIGIT in ccRCC in the high risk group were significantly higher than those in the low risk group, and the ESITIMATE tool showed that the high risk group had lower tumor purity and greater heterogeneity. Conclusion: Our study initially revealed the role of inflammatory genes in ccRCC, and provided a prognostic model risk score that could predict the prognosis of ccRCC well, and may provide more information for future research and treatment of ccRCC.
An AIE-Active Probe for Detection and Bioimaging of pH Values Based on Lactone Hydrolysis Reaction
Authors: Li Qiao , Niu Zhigang , Nan Xuying , Wang Enju 
Published: March 2022

Abstract
Cellular pH homeostasis is essential for many physiological and pathological processes. pH monitoring is helpful for the diagnosis, treatment and prevention of disorders and diseases. Herein, we developed a ratiometric fluorescent pH probe (TCC) based on a coumarin derivative containing a highly active lactone ring. TCC exhibited a typical AIE effect and emitted blue fluorescence under weak acidic condition. When under weak basic condition, the active lactone moiety underwent a hydrolysis reaction to afford a water-soluble product, which gave red-shifted emission. The emission color change from blue through cyan and then to yellow within pH 6.5–9.0 which is approximate to the biological pH range. And the fluorescence color change along with pH value is reversible. Furthermore, TCC was successfully utilized in the detection of the intracellular pH change of live HeLa cells, which indicated that TCC had practical potential in biomedical research.
Characterized profiles of gut microbiota in morphine abstinence-induced depressive-like behavior
Authors: Ji Jinshan , Yan Ni , Zhang Zhengxiang , Li Baoli , Xue Ruiyang , Dang Yonghui 
Published: March 2022

Abstract
Morphine is the most widely used analgesic for pain management worldwide. Abstinence of morphine could lead to neuropsychiatric symptoms including depression. Gut microbiota is believed to contribute to the development of depression. However, the characters and potential role of gut microbiota in morphine abstinence-induced depression remains unclear. In the present study, we first established mice models of morphine abstinence-induced depressive behavior in mice. After dividing the mice into depressive and non-depressive groups, the gut microbiota of the mice was detected by 16S rRNA gene sequencing. The difference in the diversities and abundance of the gut microbiota were analyzed between groups. Then, the representative microbial markers that could distinguish each group were identified. In addition, gene function prediction of the operational taxonomic units (OTUs) with differential abundance between the depressive and nondepressive groups after morphine abstinence was conducted. Our results suggested that four weeks from abstinence of morphine did not change the richness of the gut microbiota. While, morphine abstinence influenced the gut microbial composition. Several specific genera of gut microbiota were identified as markers for each of the groups. Interestingly, the pathway of fatty acid metabolism was found enriched in the OUTs in the depressive group compared with the nondepressive group after morphine abstinence, by gene function prediction. Our data suggested that dysbiosis of gut microbiota was associated with morphine abstinence-induced depressive behavior, possibly by implicating the fatty acid metabolism pathway.
HS3ST3A1 and CAPN8 Serve as Immune-Related Biomarkers for Predicting the Prognosis in Thyroid Cancer
Authors: Chen Zhao-Hui , Li Jun-Hui , Yue Hao-Ran , Jiang Ruo-Yu , Wang Xiao-Ning , Zhou Xue-Jie , Yu Yue , Cao Xu-Chen 
Published: March 2022

Abstract
Background: Thyroid cancer (TC) tends to be a common malignancy worldwide and results in various outcomes due to its different subtypes. The tumor microenvironment (TME) was demonstrated to play crucial roles in various malignancies, including thyroid cancer. This study combined the ESTIMATE and CIBERSORT algorithm, identified four TME-related genes, and evaluated their correlation with clinical characters. These findings revealed the malignant performance of TME in TC, and the TME-related DEGs might serve as prognostic biomarkers, which can be utilized for prediction of immunotherapy effect in patients with TC.Methods: The clinical and gene expression profiles of TC patients were collected from the TCGA dataset. The ESTIMATE algorithm was utilized to estimate stromal and immune scores and predict the level of stromal and immune cell infiltration. The differential expressed genes related to TME were filtered by the “limma” package in R, and the PPI network was constructed by a string website. KEGG pathway and GO analyses were performed to investigate biological progression and molecular functions of TME-related DEGs. Then, univariate Cox regression analysis were employed to screen four genes correlated with clinical characteristics. GSEA was conducted to assess their roles in the TME of TC. To further investigate the association between TME-related genes and tumor-infiltrating immune cells (TIICs), the CIBERSORT algorithm was performed. Finally, the malignancy behaviors of the two genes were verified by RT-qPCR, IHC, MTT, colony formation, and transwell.Results: Four TME-related DEGs, LRRN4CL, HS3ST3A1, PCOLCE2 and CAPN8, were identified and were significantly predictive of poor overall survival. KEGG and GO pathway analysis established that the TME-related DEGs were involved in immune responses and pathways in cancer. Furthermore, the malignancy behaviors of HS3ST3A1 and CAPN8 were verified by cellular functional experiments. These results revealed that the TME-related genes HS3ST3A1 and CAPN8 were able to serve as predictors of prognosis in patients with TC.Conclusion: HS3ST3A1 and CAPN8 may serve as valuable prognostic biomarkers and TME indicators in TC.
Efficacy comparison of intravascular versus intra muscular betamethasone phosphate on neonatal outcomes in the cases of imminent preterm birth.
Authors: Kashanian Maryam , Eshraghi Nooshin , Kalani Majid , Bordbar Arash , Eshraghi Nasim , Sheikhansari Narges , Kalati Mahnaz , Norouzi Sara 
Published: March 2022

Abstract
Preterm birth is the most important cause of neonatal mortality and morbidity. Finding the best treatment regimen of antenatal corticosteroids, in order to reduce neonatal complications, has been under serious concern.Objective:The purpose of the present study was to compare the efficacy of intravascular (IV) versus intra muscular (IM) betamethasone phosphate on neonatal outcomes in the cases of imminent preterm birth.Method:The study was conducted as a double-blind randomized clinical trial. 136 pregnant women with gestational age of 26- 34 weeks of pregnancy and imminent preterm birth (delivery within 24 hours) were randomly assigned into two groups. Group A received intramuscular betamethasone phosphate as a dose of 12 mg, and group B received a similar dose of betamethasone phosphate intravenously. Women were followed up to delivery, and their neonatal outcomes were compared.Results:The women of the two groups (68 women in each group), did not show a significant difference in maternal age, BMI, gravidity and parity, gestational age at the time of admission and delivery, history of miscarriage and assisted reproductive techniques, delivery route, sex and weight of newborns, and Apgar score in minutes 1 and 5. The need for NICU admission, duration of hospitalization, neonatal respiratory distress syndrome, surfactant requirement, and intubation were lower in the IV betamethasone group. There were no significant differences between the two groups according to necrotizing enterocolitis, intraventricular hemorrhage (IVH) and neonatal death.Conclusion:Using IV betamethasone, in cases where there is no enough time to complete the 24-hour betamethasone course due to the possibility of impending delivery, may reduce neonatal complications due to more rapid action.
Robust tumor detection in MRI image with edge-enhanced maximally stable extremal regions
Authors: Radak Mahyar , Bhardwaj Ritik 
Published: March 2022

Abstract
In the paper, we combine an intelligent algorithm for recognizing letters in an image and analyze its steps for tumor extraction in an MRI image. Successfully identify the border of the tumor, determine the location tumor with a limited circle, and determine whether the tumor is present or absent. Tumors vary in shape, size, and location, and may appear in different places with different intensities, so it is difficult to find the exact brain tumor. There are many algorithms, techniques, and applications for brain tumor segmentation and the overall internal structure of the brain in medical imaging, but in the proposed algorithm method, it can be extracted with high accuracy and sensitivity and extremely stable areas of maximum edge.
The HIF2α polymorphism rs4953361 is associated with impaired glucose metabolism in Han Chinese women with infertility
Authors: Zheng Xiaoya , Ma Jiani , Hu Min , Long Jian , Wei Qiang , Ren Wei 
Published: March 2022

Abstract
Objective: To evaluate HIF2α polymorphisms and glucose metabolism in a group of women with and without infertility. Design: Case–control study.Setting: Female infertility patients who visited this reproductive center and age-matched females without infertility who underwent routine health examinations from January 2020 to December 2021.Patients: The study group consisted of 148 women with infertility and 176 women without infertility as healthy controls.Intervention: We genotyped 29 single nucleotide polymorphisms (SNPs) of HIF2α by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based genotyping technology. The genetic associations were analyzed statistically.Main Outcome Measures: Allele frequency, genotype distribution and haplotype analysis of the HIF2α polymorphisms. BMI, glucose metabolism and insulin release were also measured. Results: Infertile women had elevated BMI, impaired glucose metabolism, and increased levels of plasma insulin compared to those of healthy women. SNP analysis of HIF2α revealed that the allele and genotype frequencies of rs4953361 were significantly associated with female infertility. Haplotype analysis of HIF2α polymorphism identified haplotypes TGG and TGA as being associated with female infertility. Women with the AA genotype of rs4953361 had a significantly higher BMI and postload plasma glucose and insulin levels than those of women with the GG genotype.Conclusion: This is the first study to report an association between HIF2α polymorphisms and female infertility and glucose and insulin metabolic disorder. These results require replication in larger populations but suggest that the HIF2α polymorphisms may play an important role in female obesity-related infertility. In this observational study, we did not report the results of a health care intervention on human participants. The study was approved by the Human Research Ethics Committee of the First Affiliated Hospital of Chongqing Medical University. Clinical data and peripheral blood samples were collected only after explaining the objectives of the study and obtaining a signed informed consent form.
Transcriptome analysis reveals the mechanism of chronic heat stress on meat quality of broilers
Authors: Liu Zhen , Liu Yingsen , Xing Tong , Li Jiaolong , Zhang Lin , Jiang Yun , Gao Feng 
Published: March 2022

Abstract
Background: Chronic heat stress have a negatively impacts in poultry meat quality. Although the negative impacts of chronic heat stress on meat quality have been intensively studied, previous studies have focused on metabolic changes and oxidative stress in the pectoralis major (PM) under chronic heat stress, which are partial and not all molecular steps are completely understand.Methods: A total of 144 male Arbor Acres broilers (28 d old) were randomly allocated into 3 treatment groups: (1) the normal control (NC) group, broilers were raised at 22 °C and fed a basal diet; (2) the heat stress (HS) group, birds were held at 32 °C and fed a basal diet; (3) the pair-fed (PF) group, birds were held at 22 °C and received the amount of feed equal to the feed consumed on the previous day by the HS group. The experiment was lasted for 14 days.Results: chronic heat stress heat stress decreased the average daily feed intake and average daily gain, increased feed: gain ratio (P < 0.05), and increased drip loss, cooking loss, shear force, hardness, and decreased pH, redness(a*), springiness of pectoralis major (PM) muscle (P < 0.05). Moreover, chronic heat stress decreased muscle fiber density, increased connective tissue, and lead to intracellular vacuolation. The transcriptome results indicated that that the effect of chronic heat stress on meat quality not only related to metabolism and oxidative stress, but also related to signal transduction, immune system, transport and catabolism, cell growth and death and muscle structureConclusions: This study indicate that chronic heat stress have a negative impact on growth performance, meat quality and muscle structure of the pectoralis major (PM) in broilers and provide a comprehensive understanding of the mechanism of chronic heat stress-induced deterioration of broiler meat quality at the transcriptional level.
First Report of a Novel Fusion Protein, IL-4/IL-34/IL-10, Which Promotes Cardiac Allograft Survival Mice Through Alloregulation
Authors: Lee Young S. , Cheng I-Ting , Godoy-Ruiz Raquel , Weber David J. , Scalea Joseph R. 
Published: March 2022

Abstract
Immune mediated graft loss still represents a major risk to transplant recipients. Creative approaches to immunosuppression that exploit the recipient’s own alloregulatory mechanisms could reduce the need for pharmacologic immunosuppression and potentially induce immune tolerance. In the process of studying recipient derived myeloid derived suppressor cells (MDSCs), we identified key alloregulatory MDSC mechanisms, mediated by isolatable proteins IL-4, IL-34, and IL-10. We sought to purify these proteins and fuse them for subsequent infusion into transplant recipients as a means of inducing an alloregulatory response. We leveraged molecular engineering technology to create a fusion protein (FP) of three cytokine coding sequences of IL-4, IL-34, and IL-10 and demonstrated their expressions by Western Blot analysis. Following purification, we tested whether FP IL-4/IL-34/IL-10 (FP1) can protect heart transplant allografts. Injection of FP1 significantly prolonged allogeneic cardiac graft survival in a dose-dependent fashion and the increase of graft survival time exceeded survival attributable to IL-34 alone. In vitro, MDSCs cells were expanded by FP1 treatment. However, FP1 did not directly inhibit T cell proliferation in vitro. In conclusion, newly developed FP1 improves the graft survival in cardiac transplantation mouse model. Additional work to optimize FP1 or include other novel proteins could supplement current treatment options for transplant patients.
Significant Effects of Dead Trees on Active Organic Carbon Components and Microbial Communities in Subtropical Forests
Authors: Jiang XingJian , Dang Peng , Yan Wende , Zheng Wei , Xu Yicheng , Chen Yazhen 
Published: March 2022

Abstract
As a vital component of the forest ecosystem, dead wood plays an important role in forest regeneration, species variety, water conservation, soil erosion avoidance, and the carbon cycle. At the moment, dead wood research is mostly concerned with the storage of dead wood in forests and the change of nutritional components throughout the decomposition process. There have been few investigations on the changes in soil active organic carbon components and bacterial community structure during the breakdown of dead wood. To investigate the impact of dead wood on soil active organic carbon components and the structure of microbial communities. we selected the soil under three different decay grades of Castanopsis eyrei dead wood and the forest soil without dead wood as the control. The basic physical and chemical indexes of soil, the composition of soil active organic carbon and the structure of microbial community were measured. Compared with the control group, dead wood mulching significantly increased the content of soil organic carbon and total nitrogen (P < 0.05), and dead wood significantly affected the content of soil active organic carbon (P < 0.05). The easily oxidized organic carbon, microbial biomass carbon and organic carbon under the severe decay dead wood were significantly higher than those in the control (P < 0.05), and the content of activated carbon increased with the increase of decay grade. The relative abundance of Actinobacteria, Chloroflexi, WPS-2, Patescibacteria and Bacteroidetes increased with the increase of decay grade of dead wood, and the relative abundance of soil bacteria under dead wood was higher than that of the control. Through the redundancy analysis of environmental factors and soil microbial community, we found soil total nitrogen and pH are the key factors driving microbial community. The distribution of soil bacterial community under light rotten dead wood is similar to that soil without dead wood, which is significantly different from that under moderate and severe rotten dead wood. The abundance of soil bacterial community and soil active organic carbon components are most affected by heavy rotten dead wood. Soil active organic carbon was significantly negatively correlated with Acidobacteria(P < 0.01), and positively correlated with Ktedonobacteria, Bacteroidia, Saccharimonadia and TK10(P < 0.05). The above research results show that dead wood has a great impact on soil physical and chemical properties and microbial community structure. Our study improves the impact of dead wood on soil activated carbon components and microbial community, and provides a theoretical basis for sustainable forest management.