bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variability in the Carbon and Nitrogen Uptake Rates of Phytoplankton Associated With Wind Speed and Direction in the Marian Cove, Antarctica
Authors: Kim Bo Kyung , Jeon Misa , Park Sang-Jong , Kim Hyun-Cheol , Min Jun-Oh , Park Jisoo , Ha Sun-Yong 
Published: July 2022

Quantifying the temporal variability in phytoplankton productivity is essential for improving our understanding of carbon (C) and nitrogen (N) dynamics and energy flows in natural aquatic ecosystems. Samples were collected at three-day intervals from December 2018 to January 2019 from fixed station in Marian Cove, Antarctica to determine the C and N (NO3- and NH4+) uptake by phytoplankton. Considerable fluctuations in the total C and N productivities were observed, which led to dynamic changes in the phytoplankton communities and a stronger coupling between the phytoplankton biomass. The increased rate of NO3- uptake coincided with an enhanced C uptake mainly by microphytoplankton (&gt;20 µm), followed by an increase in NH4+ uptake towards the end of sampling period. However, the &lt;2 µm fraction (picophytoplankton) showed little variation in C and NO3- uptake, and the proportions of assimilated NH4+ contributed to more than half of the total assimilated inorganic N. The increased NH4+ did not increase the total phytoplankton biomass and C production. Interestingly, after January 9 (maximum chlorophyll a, C, and N uptake) there was a shift to a predominantly easterly wind (&gt;6 m s-1), which rapidly decreased the total chl-a, C and N uptake rate to ~4% of the highest values (0.6 mg m-3, 1.0 mg C m-3 h-1, 0.1 mg N m-3 h-1, respectively) on January 12. The phytoplankton community was also replaced by neritic and ice-related species. These findings suggest that strong temporal shifts in phytoplankton C and N assimilation are strongly influenced by external forces (wind stress).
Is All Seagrass Habitat Equal? Seasonal, Spatial, and Interspecific Variation in Productivity Dynamics Within Mediterranean Seagrass Habitat
Authors: Ward Emma A. , Aldis Charlotte , Wade Tom , Miliou Anastasia , Tsimpidis Thodoris , Cameron Tom C. 
Published: July 2022

Seagrass meadows’ ability to capture carbon through sequestering autochthonous carbon via photosynthesis means they could represent a potential nature-based solution to rising carbon emissions. In multispecies seagrass communities, and due to species introduction or predicted range shifts, it is important to know which species deliver different carbon sequestration gains to inform conservation actions. Large benthic chamber experiments (volume = 262L) assessed the seasonal and spatial variation in metabolism dynamics of the endemic and dominant Mediterranean seagrass, P. oceanica whilst small benthic chamber experiments (volume = 7L) compared the dynamics between, P. oceanica the native C. nodosa and non-native H. stipulacea. Within shallow P. oceanica edge habitat lower Net Apparent Productivity (NAP) occurs in autumn (x¯ = 1.3, SD ± 2.95 O2 mmol m-2 d-1) compared to summer (x¯ = 9.9, SD ± 2.75 O2 mmol m-2 d-1 corresponding with periods of light limiting and light saturating conditions, but it remains overall autotrophic annually (2.3 C mol m-2 yr-1). However, spatial heterogeneity exists, the center areas of P. oceanica were more productive (NAP x¯ =19.7, SD± 3.83 O2 mmol m-2 d-1) compared to edge habitat with spatial changes in productivity relating to plant surface area (96%), shoot density (81%), blade length (72%) and seagrass percentage cover (64%). Under comparative conditions in a sparse multispecies area of the meadow the species demonstrated different capacities for carbon fixation. H. stipulacea was carbon positive and P. oceanica fluctuated between positive and negative carbon balance suggesting both can maintain a balance between carbon fixation and carbon utilised for metabolic activity. In contrast the C. nodosa here would be expected to deteriorate as it was utilising carbon more than it was fixing (NAPN2 (x¯ = -0.0012, SD ± 0.0007 O2 mmol cm-2 d-1). This study demonstrates that not all seagrass habitat is equal. If seagrass meadows are to play a part in mitigating CO2 emissions, variability in primary productivity within seagrass meadows needs to be accounted for to produce accurate total fixed carbon estimates, and subsequently autochthonous carbon sequestration estimates. This means seagrass meadow species composition and the condition of these meadows must be better understood.
Trend and Geographic Variation in Incidence and Prevalence of Inflammatory Bowel Disease in Regions Across China: A Nationwide Employee Study Between 2013 and 2016
Authors: Yang Hong , Zhou Runing , Bai Xiaoyin , Guo Mingyue , Ruan Gechong , Wang Li , Qian Jiaming 
Published: July 2022

BackgroundIncidence and prevalence rates and trends of inflammatory bowel disease (IBD) in China remain largely unknown.ObjectiveThis study aimed to estimate the nationwide prevalence and incidence of IBD and identify its noticeable trends in China between 2013 and 2016.MethodsWe conducted a population-based analysis using data from the National Urban Employee Basic Medical Insurance database. Patients with at least three claims of IBD diagnosis were identified. A Joinpoint regression model was used to analyze the annual percent change (APC) of the age-standardized incidence and prevalence.ResultsThe age-standardized prevalence of Crohn's disease (CD) increased from 1.59/100,000 in 2013 to 3.39/100,000 (p &lt; 0.05) in 2016, and that of ulcerative colitis (UC) increased from 8.72/100,000 to 17.24/100,000 (p &lt; 0.05) during the period, with a UC/CD ratio of 5.09 in 2016. The age-standardized incidence of CD varied between 0.82/100,000 and 0.97/100,000 (p = 0.9), whereas that of UC slightly increased from 4.54/100,000 to 4.85/100,000 (p = 0.7). The eastern region of China had the highest incidence and prevalence, and the western region had the lowest rates, in both UC and CD, showing an east-to-west gradient.ConclusionThe incidence and prevalence of IBD in most urban regions in China had an emerging trend over the study period, and an east-to-west gradient was observed, which indicated a greater burden in eastern China. Efforts to improve prevention strategies and promote awareness of IBD are needed, particularly in young men who are at higher risk for CD.
Altered functional connectivity within default mode network after rupture of anterior communicating artery aneurysm
Authors: Chen Fuxiang , Kang Yaqing , Yu Ting , Lin Yuanxiang , Dai Linsun , Yu Lianghong , Wang Dengliang , Sun Xi , Kang Dezhi 
Published: July 2022

BackgroundRupture of anterior communicating artery (ACoA) aneurysm often leads to cognitive impairment, especially memory complaints. The medial superior frontal gyrus (SFGmed), a node of the default mode network (DMN), has been extensively revealed to participate in various cognitive processes. However, the functional connectivity (FC) characteristics of SFGmed and its relationship with cognitive performance remain unknown after the rupture of the ACoA aneurysm.MethodsResting-state functional MRI (fMRI) and cognitive assessment were acquired in 27 eligible patients and 20 controls. Seed-based FC between unilateral SFGmed and the rest of the brain was calculated separately, and then compared their intensity differences between the two groups. Furthermore, we analyzed the correlation between abnormal FC and cognitive function in patients with ruptured ACoA aneurysm.ResultsCognitive impairment was confirmed in 51.9% of the patients. Compared with the controls, patients suffering from ruptured ACoA aneurysm exhibited a similar FC decline between each side of SFGmed and predominant nodes within DMN, including the precuneus, angular gyrus, cingulate cortex, left hippocampus, left amygdala, left temporal pole (TPO), and left medial orbitofrontal cortex (mOFC). Besides, significantly decreased FC of left SFGmed and left insula, right middle temporal gyrus (MTG), as well as right mOFC, were also found. In addition, only enhanced insular connectivity with right SFGmed was determined, whereas increased FC of the left SFGmed was not observed. Correlation analyses showed that lower total cognitive performance or stronger subjective memory complaints were related to reduced connectivity in the SFGmed and several cortical regions such as the angular gyrus and middle cingulate cortex (MCC).ConclusionOur results suggest that patients with ruptured ACoA aneurysm exist long-term cognitive impairment and intrinsic hypoconnectivity of cognition-related brain regions within DMN. Deactivation of DMN may be a potential neural mechanism leading to cognitive deficits in these patients.
The association between ST8SIA2 gene and behavioral phenotypes in children with autism spectrum disorder
Authors: Yang Xiaolei , Li Lin , Chai Xuejiao , Liu Jicheng 
Published: July 2022

ObjectiveST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (ST8SIA2) encodes a type II membrane protein that is thought to catalyze the transfer of sialic acid (SA) from CMP-SA to N-linked oligosaccharides and glycoproteins. Some population and animal studies have indicated an association between the ST8SIA2 gene and autism spectrum disorder (ASD). However, there is limited information on the correlation between ST8SIA2 and autistic behavioral symptoms.MethodsIn this study, 69 ASD and 76 normal control children who were age- and sex-matched were recruited. ST8SIA2 expression and methylation levels were measured by reverse transcription quantitative real-time PCR and pyrosequencing, respectively, and the behavioral phenotypes of ASD children were assessed.ResultsThe ASD group had lower ST8SIA2 gene expression levels than the control group [t(0.05/2,143) = 2.582, p = 0.011]. Moreover, ST8SIA2 expression levels were positively correlated with daily life skills (rs = 0.381, p = 0.008) and negatively associated with stereotyped behaviors in the ASD group (rs = -0.510, p = 0.004). The methylation levels of the Chr. 15: 92984625 and Chr. 15: 92998561 sites of the ST8SIA2 gene in ASD children were higher than those of controls. The Chr. 15: 92984625 site was positively correlated with the stereotyped behaviors of ASD children (rs = 0.41, p = 0.039).ConclusionThis study provides a scientific basis to elucidate the relationship between the ST8SIA2 gene and behavioral phenotypes of ASD.
Impact of MMP2 rs243849 and rs14070 genetic polymorphisms on the ischemic stroke susceptibility in Chinese Shaanxi population
Authors: Li Shilin , Yang Shiyao , Zhang Xiaobo , Zhang Yu , Zhang Jie , Zhang Xiao , Li Weiping , Niu Xiaochen , Shi Wenzhen , Zhang Gejuan , Chang Mingze , Tian Ye 
Published: July 2022

BackgroundIschemic stroke (IS) is a complex neurological disease affected by genetics and environment. Matrix metalloproteinase-2 (MMP2) is involved in extracellular matrix (ECM) degradation, inflammation and angiogenesis to regulate the development and recovery of IS.PurposesThe aim of this study was to explore the association of rs1053605, rs243849 and rs14070 in MMP2 with the risk of IS in Chinese Shaanxi population.MethodsIn this study, 677 IS patients and 681 normal controls were recruited. Rs1053605, rs243849 and rs14070 in MMP2 were genotyped. Logistic regression analysis was applied to evaluate the association of rs1053605, rs243849 and rs14070 in MMP2 with IS susceptibility and the association of environmental factors with MMP2 genetic susceptibility to IS.ResultsThe results of the overall analysis demonstrated that rs14070 in MMP2 significantly reduced the risk of IS in Chinese Shaanxi population (OR = 0.767, 95% CI = 0.619–0.952, P = 0.016). Subgroup analysis illustrated that rs243849 in MMP2 evidently increased the risk of IS among drinkers, while rs14070 in MMP2 apparently reduced IS susceptibility among females, participants with aged &gt;55, smokers and drinkers.ConclusionsCollectively, rs243849 and rs14070 in MMP2 were significantly associated with the risk of IS in Chinese Shaanxi population, and the effect of MMP2 to IS may be associated with its genetic susceptibility.
The effect of endovascular baroreflex amplification on central sympathetic nerve circuits and cerebral blood flow in patients with resistant hypertension: A functional MRI study
Authors: Groenland Eline H. , van Kleef Monique E. A. M. , Hendrikse Jeroen , Spiering Wilko , Siero Jeroen C. W. 
Published: July 2022

BackgroundEndovascular baroreflex amplification (EVBA) by implantation of the MobiusHD is hypothesized to lower blood pressure by decreasing sympathetic activity through the mechanism of the baroreflex. In the present exploratory study we investigated the impact of MobiusHD implantation on central sympathetic nerve circuits and cerebral blood flow (CBF) in patients with resistant hypertension.Materials and methodsIn thirteen patients, we performed blood oxygenation level-dependent functional magnetic resonance imaging (BOLD fMRI) at rest and during Valsalva maneuvers, before and 3 months after EVBA. Data were analyzed using a whole-brain approach and a brainstem-specific analysis. CBF was assessed using arterial spin labeling MRI.ResultsResting-state fMRI analysis did not reveal significant differences in functional connectivity at 3 months after EVBA. For the Valsalva maneuver data, the whole-brain fMRI analysis revealed significantly increased activation in the posterior and anterior cingulate, the insular cortex, the precuneus, the left thalamus and the anterior cerebellum. The brainstem-specific fMRI analysis showed a significant increase in BOLD activity in the right midbrain 3 months after EVBA. Mean gray matter CBF (partial volume corrected) decreased significantly from 48.9 (9.9) ml/100 gr/min at baseline to 43.4 (13.0) ml/100 gr/min (p = 0.02) at 3 months.ConclusionsThis first fMRI pilot study in patients with resistant hypertension treated with EVBA showed a significant increase in BOLD activity during the Valsalva maneuver in brain regions related to sympathetic activity. No notable signal intensity changes were observed in brain areas involved in the baroreflex circuit. Future randomized controlled studies are needed to investigate whether the observed changes are directly caused by EVBA.Clinical trial registrationwww.clinicaltrials.gov, identifier: NCT02827032.
A novel perspective of associativity of upper limb motor impairment and cortical excitability in sub-acute and chronic stroke
Authors: Saini Megha , Singh Neha , Kumar Nand , Srivastava M. V. Padma , Mehndiratta Amit 
Published: July 2022

BackgroundThe global inclination of stroke onset in earlier years of life and increased lifespan have resulted in an increased chronic post-stroke-related disability. The precise and simplistic approach such as the correlation of Fugl-Meyer Assessment (FMA) with Transcranial Magnetic Stimulation (TMS) parameters, Resting Motor Threshold (RMT) and Motor Evoked Potential (MEP), in patients with stroke might play a critical role, given the prognostic value of MEP, a measure of cortical excitability, and might be the key point in prescribing appropriate therapeutic strategies.ObjectiveThe study aimed to determine the correlation of FMA-based impairment in the upper extremity function specifically of the wrist and hand with respect to the neurophysiological parameters of corticospinal tract integrity.Materials and methodsThe Institutional Review Board approved the study and 67 (n) patients with stroke were enrolled in the Department of Neurology, AIIMS, New Delhi, India. The motor assessment was performed on patients by the upper extremity subset of Fugl-Meyer Assessment (FMA) and the clinical history was obtained. RMT and MEP of Extensor Digitorum Communis (EDC) muscle were measuredviaTMS.ResultsA significant positive correlation was observed between Fugl-Meyer Assessment Wrist/Hand (FMA W/H) and MEP scores (r= 0.560, &lt;0.001). Also, Fugl-Meyer Assessment Upper Extremity (FMA UE) scores demonstrated a moderate positive association with MEP responsiveness (r= 0.421, &lt;0.001).ConclusionMEP of the EDC muscle was found to be associated with sensorimotor control as measured by FMA. Moreover, FMA W/H score values might be a better prognostic indicator of EDC MEP responsiveness. Interestingly, a novel element comprising the range of FMA UE and FMA W/H components was observed to be a potential indicator of MEP responsiveness and could also indicate establishing FMA as a surrogate for TMS in resource-limited settings for prognostification.
Effect of depression disorder on the efficacy and quality of life of first-line chemotherapy combined with immunotherapy in oncogene-driver negative NSCLC patients
Authors: Li Wen , Bi Ziran , Wu Junxu , Duan Xu , Pang Lulian , Jing Yanyan , Yin Xiangxiang , Cheng Huaidong 
Published: July 2022

ObjectiveThe current research was to assess the relevance between depression disorder and first-line chemotherapy combined with immunotherapy, quality of life in patients with oncogene-driver negative non-small cell cancer (NSCLC).MethodsNSCLC patients (33 with depression disorder and 34 with no depression disorder) who was received first-line chemotherapy combined with immunotherapy performed Zung Self-rating Depression Scale (SDS) and European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30).ResultsThe Progression-Free Survival (PFS) of depression disorder group survivors were lower than these of no depression disorder group survivors (HR, 0.352; 95% CI, 0.201-0.617; P&lt;0.05). The statistical significant was revealed about the Objective Response Rate (ORR) and Disease Control Rate (DCR) in two groups (P&lt;0.05). The quality of life scores of NSCLC patients in no depression disorder group was significantly higher after chemotherapy combined with immunotherapy, and manifested as 92.7 ± 28 vs. 76.3 ± 23.3 (t=8.317, P&lt;0.05), and had a significant difference.ConclusionDepression disorder in oncogene-driver negative NSCLC patients influence the curative effect of chemotherapy combined with immunotherapy, and depression disorder was significantly negatively associated with quality of life following chemotherapy combined with immunotherapy.
Radionuclide 131I-labeled albumin-indocyanine green nanoparticles for synergistic combined radio-photothermal therapy of anaplastic thyroid cancer
Authors: Zhang Xuemei , Yan Ziyu , Meng Zhaowei , Li Ning , Jia Qiang , Shen Yiming , Ji Yanhui 
Published: July 2022

ObjectivesAnaplastic thyroid cancer (ATC) cells cannot retain the radionuclide iodine 131 (131I) for treatment due to the inability to uptake iodine. This study investigated the feasibility of combining radionuclides with photothermal agents in the diagnosis and treatment of ATC.Methods131I was labeled on human serum albumin (HSA) by the standard chloramine T method. 131I-HSA and indocyanine green (ICG) were non-covalently bound by a simple stirring to obtain 131I-HSA-ICG nanoparticles. Characterizations were performed in vitro. The cytotoxicity and imaging ability were investigated by cell/in vivo experiments. The radio-photothermal therapy efficacy of the nanoparticles was evaluated at the cellular and in vivo levels.ResultsThe synthesized nanoparticles had a suitable size (25–45 nm) and objective biosafety. Under the irradiation of near-IR light, the photothermal conversion efficiency of the nanoparticles could reach 24.25%. In vivo fluorescence imaging and single-photon emission CT (SPECT)/CT imaging in small animals confirmed that I-HSA-ICG/131I-HSA-ICG nanoparticles could stay in tumor tissues for 4–6 days. Compared with other control groups, 131I-HSA-ICG nanoparticles had the most significant ablation effect on tumor cells under the irradiation of an 808-nm laser.ConclusionsIn summary, 131I-HSA-ICG nanoparticles could successfully perform dual-modality imaging and treatment of ATC, which provides a new direction for the future treatment of iodine-refractory thyroid cancer.
MiR-520a-5p/PPP5C regulation pattern is identified as the key to gemcitabine resistance in pancreatic cancer
Authors: Fu Ruibiao , Shao Qian , Yang Bin , Chen Yan , Ye Qinghuang , Chen Xi , Zhu Jinhui 
Published: July 2022

ObjectiveTo explore the effects of the expression level of miR-520-5p/PPP5C in pancreatic cancer cells and exosomes on cell viability, angiogenesis, autophagy, which involved in the mechanism of gemcitabine resistance in pancreatic cancer.MethodsAPSC-1 cell line was treated with gemcitabine, after which its exosomes were extracted	for NTA assay. Subsequently, the drug resistance of APSC-1 cells was assayed using CCK8, as well as the activity of HUVEC cells treated with exosomes from each group of APSC-1 cells after drug resistance treatment as well as overexpression treatment. Five groups of HUVEC cells treated with exosomes were subjected to in vitro tubule formation assay. levels of PPP5C in each group of ASPC-1 cells and their exosomes, levels of overexpressed PPP5C, and related exosomal proteins were examined by WB. mRNA expression levels of PPP5C and levels of miR-520a were examined by qPCR The relationship between miR-520a-5p and PPP5C was investigated. After that, the autophagy of PPP5C was detected. Finally, it was analyzed by TCGA database for survival prognosis analysis.ResultsAPSC-1 cells had an IC50 value of 227.1 μM for gemcitabine, elevated PPP5C expression, drug resistance, and enhanced HUVEC cell activity; exosomes CD9, CD63, and CD81 were significantly expressed in all groups; meanwhile, enhanced PPP5C expression not only promoted in vitro tubule formation but also increased autophagy levels; meanwhile, its relationship with miR-520-5p and There was a targeted inhibitory relationship between its level and miR-520-5p and PPP5C, and its elevated level also led to a decrease in the survival level of patients over 3-5 years.ConclusionPPP5C has a prognostic role in pancreatic cancer by promoting the value-added and invasion of pancreatic cancer cells, and a targeted inhibitory relationship between miR-520-5p and PPP5C was found.
Successful sequential tyrosine kinase inhibitors to overcome a rare compound of EGFR exon 18–18 and EGFR amplification: A case report
Authors: Wang Pascal , Fabre Emmanuelle , Martin Antoine , Chouahnia Kader , Benabadji Ambre , Matton Lise , Duchemann Boris 
Published: July 2022

BackgroundNew mutational detection techniques like next-generation sequencing have resulted in an increased number of cases with uncommon mutation and compound mutations [3%–14% of all epidermal growth factor receptor (EGFR) mutations]. In rare exon 18 mutations (3%–6%), G719X and E709X represent the majority, but CMut associating these exon 18 points mutations are even rarer, making the understanding of the impact of epidermal growth factor receptor tyrosine kinase inhibitors still limited. Three generations of EGFR tyrosine kinase inhibitors (TKIs) are available to target EGFR mutations, but according to the types of mutations, the sensitivity to TKI is different. Afatinib, osimertinib, and neratinib have showed some effectiveness in single exon 18, but no report has precisely described their efficiency and acquired mechanism of resistance in a CMut of exon 18–18 (G719A and E709A).Case presentationWe report a case of a 26-year-old woman with bilateral advanced adenocarcinoma of the lung harboring a compound mutation associating G719A and E709A in exon 18, who developed an EGFR amplification as resistance mechanism to osimertinib. She presented a significant clinical and morphological response under sequential TKIs treatment (afatinib, osimertinib, and then neratinib).ConclusionA non-small cell lung cancer (NSCLC) with rare compound mutation exon 18–exon 18 (G719A and E709A) and EGFR amplification can be overcome with adapted sequential second- and third-generation TKIs. This report has potential implications in guiding decisions for the treatment of these rare EGFR mutations.