bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin
Authors: Yu Changhui , Huang Wufeng , Zhou Zicong , Liang Shixiu , Zhou Zili , Liu Jieyi , Zhao Haijing , Liu Laiyu , Dong Hangming , Zou Fei , Cai Shaoxi 
Published: March 2022

Abstract
Background
Up-regulation of aerobic glycolysis has been reported as a characterization of asthma and facilitates airway inflammation. We has been previously reported that short isoform thymic stromal lymphopoietin (sTSLP) could reduce inflammation in asthmatic airway epithelial cells. Here we wanted to investigate whether the inhibition of sTSLP on asthma is related to aerobic glycolysis.

Methods
Asthmatic model was established in challenging Male BALB/c mice and 16-HBE (human bronchial epithelial) cell line with house dust mite (HDM). Indicators of glycolysis were assessed to measure whether involve in sTSLP regulating airway epithelial cells inflammation in asthmatic model in vivo and in vitro.

Results
sTSLP decreased inflammation of asthmatic airway and aerobic glycolysis in mice. HDM or long isoform thymic stromal lymphopoietin (lTSLP) promoted HIF-1α expression and aerobic glycolysis by miR-223 to target and inhibit VHL (von Hippel-Lindau) expression 16-HBE. Inhibition of aerobic glycolysis restrained HDM- and lTSLP-induced inflammatory cytokines production. sTSLP along had almost no potential to alter aerobic glycolysis of 16-HBE. But sTSLP decreased LDHA (lactate dehydrogenase A) and LD (Lactic acid) levels in BALF, and HIF-1α and LDHA protein levels in airway epithelial cells of asthma mice model. lTSLP and sTSLP both induced formation of TSLPR and IL-7R receptor complex, and lTSLP obviously facilitated phosphorylation of JAK1, JAK2 and STAT5, while sTSLP induced a little phosphorylation of JAK1 and STAT5.

Conclusion
We identified a novel mechanism that lTSLP could promote inflammatory cytokines production by miR-223/VHL/HIF-1α pathway to upregulate aerobic glycolysis in airway epithelial cells in asthma. This pathway is suppressed by sTSLP through occupying binding site of lTSLP in TSLPR and IL-7R receptor complex.
Short-term exposure to ambient air pollution and pneumonia hospital admission among patients with COPD: a time-stratified case-crossover study
Authors: Lu Wenfeng , Tian Qi , Xu Ruijun , Zhong Chenghui , Qiu Lan , Zhang Han , Shi Chunxiang , Liu Yuewei , Zhou Yun 
Published: March 2022

Abstract
Background
Pneumonia is a major contributor to hospital admission for patients with chronic obstructive pulmonary disease (COPD). However, evidence for acute effects of ambient air pollution exposure on pneumonia hospital admission among patients with COPD is scarce. We aimed to examine the association between short-term exposure to ambient air pollution and pneumonia hospital admission among patients with COPD.

Methods
We enrolled COPD cases aged ≥ 60 years old and further filtered those who were admitted into hospitals from pneumonia during 2016–2019 in Guangdong province, China for main analysis. A time-stratified case-crossover design was applied to investigate the association and conditional logistic regression model was used for data analysis. We performed inverse distance weighting method to estimate daily individual-level exposure on particulate matter with an aerodynamic diameter ≤ 2.5 μm (PM2.5), particulate matter with an aerodynamic diameter ≤ 10 μm (PM10), sulfur dioxide (SO2), nitrogen dioxide (NO2), carbon monoxide (CO), and ozone (O3) based on personal residential addresses.

Results
We included 6473 pneumonia hospital admissions during the study period. Each interquartile range (IQR) increase in PM2.5 (lag 2; IQR, 22.1 μg/m3), SO2 (lag 03; IQR, 4.2 μg/m3), NO2 (lag 03; IQR, 21.4 μg/m3), and O3 (lag 04; IQR, 57.9 μg/m3) was associated with an odds ratio in pneumonia hospital admission of 1.043 (95% CI: 1.004–1.083), 1.081 (95% CI: 1.026–1.140), 1.045 (95% CI: 1.005–1.088), and 1.080 (95% CI: 1.018–1.147), respectively. Non-linear trends for PM2.5, PM10, and SO2 were observed in the study. Sex, age at hospital admission, and season at hospital admission did not modify the associations.

Conclusions
We found significantly positive associations of short-term exposure to PM2.5, SO2, NO2, and O3 with pneumonia hospital admission among COPD patients. It provides new insight for comprehensive pneumonia prevention and treatment among COPD patients.
Microbial and molecular differences according to the location of head and neck cancers
Authors: Kim Yun Kyeong , Kwon Eun Jung , Yu Yeuni , Kim Jayoung , Woo Soo-Yeon , Choi Hee-Sun , Kwon Munju , Jung Keehoon , Kim Hyung-Sik , Park Hae Ryoun , Lee Dongjun , Kim Yun Hak 
Published: March 2022

Abstract
Background
Microbiome has been shown to substantially contribute to some cancers. However, the diagnostic implications of microbiome in head and neck squamous cell carcinoma (HNSCC) remain unknown.

Methods
To identify the molecular difference in the microbiome of oral and non-oral HNSCC, primary data was downloaded from the Kraken-TCGA dataset. The molecular differences in the microbiome of oral and non-oral HNSCC were identified using the linear discriminant analysis effect size method.

Results
In the study, the common microbiomes in oral and non-oral cancers were Fusobacterium, Leptotrichia, Selenomonas and Treponema and Clostridium and Pseudoalteromonas, respectively. We found unique microbial signatures that positively correlated with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in oral cancer and positively and negatively correlated KEGG pathways in non-oral cancer. In oral cancer, positively correlated genes were mostly found in prion diseases, Alzheimer disease, Parkinson disease, Salmonella infection, and Pathogenic Escherichia coli infection. In non-oral cancer, positively correlated genes showed Herpes simplex virus 1 infection and Spliceosome and negatively correlated genes showed results from PI3K-Akt signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, ECM-receptor interaction and Dilated cardiomyopathy.

Conclusions
These results could help in understanding the underlying biological mechanisms of the microbiome of oral and non-oral HNSCC. Microbiome-based oncology diagnostic tool warrants further exploration.
ADHD symptoms and school impairment history in parents of ADHD children are a fundamental diagnostic and therapeutic clue
Authors: Wiel Luisa Cortellazzo , Rispoli Francesco , Peccolo Giulia , Rosolen Valentina , Barbi Egidio , Skabar Aldo 
Published: March 2022

AbstractBackgroundAttention Deficit and Hyperactivity Disorder (ADHD) is a multi-factorial condition, with inheritance playing a major role. Recognizing parents’ ADHD represents a clue not only for an earlier diagnosis of the disease in their children, but also to optimize psycho-educational therapy outcomes, by addressing the impairment of parenting related to untreated ADHD. This study aimed to assess the frequency of features suggestive of ADHD during childhood among parents of affected children, and the presence of school and emotional impairment.MethodsWe administered the Wender Utah Rating Scale-25, a self-assessment tool for the retrospective identification of symptoms consistent with ADHD during childhood, to a cohort of 120 parents of 60 children with ADHD, and to a consistent number of “controls”.ResultsThe WURS-25 proved positive in 49.1% of fathers and 30.0% of mothers of ADHD patients, compared to 1.7% of fathers and 1.7% of mothers of non-ADHD patients (p < 0.0001).The questions addressing learning and emotional impairment provided significantly higher scores in parents with an overall positive test compared to those with negative test (p < 0.0001).ConclusionsThis study demonstrates a remarkably high rate of symptoms consistent with ADHD during childhood in parents of affected children. Physicians should be aware that this is a relevant anamnestic clue and, given the relevance of parents’ role in the management of children with ADHD, an important issue to address in order to optimize patients’ treatment.
A deep learning MRI approach outperforms other biomarkers of prodromal Alzheimer’s disease
Authors: Feng Xinyang , Provenzano Frank A. , Small Scott A. ,   for the Alzheimer’s Disease Neuroimaging Initiative
Published: March 2022

Abstract
Background
The three core pathologies of Alzheimer’s disease (AD) are amyloid pathology, tau pathology, and neurodegeneration. Biomarkers exist for each. Neurodegeneration is often detected by neuroimaging, and we hypothesized that a voxel-based deep learning approach using structural MRI might outperform other neuroimaging methods.

Methods
First, we implement an MRI-based deep learning model, trained with a data augmentation strategy, which classifies Alzheimer’s dementia and generates class activation maps. Next, we tested the model in prodromal AD and compared its performance to other biomarkers of amyloid pathology, tau pathology, and neuroimaging biomarkers of neurodegeneration.

Results
The model distinguished between controls and AD with high accuracy (AUROC = 0.973) with class activation maps that localized to the hippocampal formation. As hypothesized, the model also outperformed other neuroimaging biomarkers of neurodegeneration in prodromal AD (AUROC = 0.788) but also outperformed biomarkers of amyloid (CSF Aβ = 0.702) or tau pathology (CSF tau = 0.682), and the findings are interpreted in the context of AD’s known anatomical biology.

Conclusions
The advantages of using deep learning to extract biomarker information from conventional MRIs extend practically, potentially reducing patient burden, risk, and cost.
Engineering strategies for customizing extracellular vesicle uptake in a therapeutic context
Authors: Esmaeili Abazar , Alini Mauro , Baghaban Eslaminejad Mohamadreza , Hosseini Samaneh 
Published: March 2022

AbstractExtracellular vesicles (EVs) are advanced therapeutic strategies that can be used to efficiently treat diseases. Promising features of EVs include their innate therapeutic properties and ability to be engineered as targeted drug delivery systems. However, regulation of EV uptake is one challenge of EV therapy that must be overcome to achieve an efficient therapeutic outcome. Numerous efforts to improve the factors that affect EV uptake include the selection of a cell source, cell cultivation procedure, extraction and purification methods, storage, and administration routes. Limitations of rapid clearance, targeted delivery, and off-targeting of EVs are current challenges that must be circumvented. EV engineering can potentially overcome these limitations and provide an ideal therapeutic use for EVs. In this paper, we intend to discuss traditional strategies and their limitations, and then review recent advances in EV engineering that can be used to customize and control EV uptake for future clinical applications.
Pupation inhibition and larvicidal activity of tyrosinase on Culex pipiens third-instar larvae
Authors: Shalaby Hatem A. , Ashry Heba M. , Saad Moataza M. , El Namaky Amira H. , Hassen Marwa T. 
Published: March 2022

Abstract
Background
Mosquitoes are considered to be the main vector of a variety of diseases in both humans and domesticated animals. The development of insecticide resistance and the limitations of traditional insecticide-based strategies have resulted in significant efforts to develop eco-friendly, alternative methods.

Main body
In this study, nine fungi species were screened to produce tyrosinase enzyme. This was done in order to evaluate its inhibitory activity against Culex pipiens third-instar larvae. The extracellular tyrosinase was produced by five strains. Aspergillus tamarii NRC3 was found to possess the highest tyrosinase activity and was therefore used in this study. Some factors were studied to enhance the production of the enzyme. The enzyme was partially purified using ammonium sulfate at a 70% saturation, giving 1.861 purification fold. The toxicity on Culex pipiens varied, depending upon the tyrosinase concentration and the period of exposure. In addition, notable histological effects were seen in the midgut region.

Conclusions
A concentration of 80% on third-stage larvae showed 90% inhibition in the formation of pupae at 72 h post-treatment. In addition, a significant cellular microvillus disruption was seen in the midgut region at 24 h post-treatment.
Native bacteria for field biocontrol of black root rot in Egyptian strawberry
Authors: Abd-El-Kareem Farid , Elshahawy Ibrahim E. , Abd-Elgawad Mahfouz M. M. 
Published: March 2022

Abstract
Background
Increasing cultivation of strawberry in Egypt has boosted efforts to increase its yield. Biocontrol agents (BCAs) may avoid side effects and health risks caused by chemical fungicides used to control black root rot disease in strawberry. Some BCAs control the disease and augment strawberry yield, but additional research is needed to fit BCAs into emerging control strategies. The impact of six bacterial isolates of Paenibacillus polymyxa and Bacillus brevis on this disease and on berry yield is reported and compared to a common chemical fungicide.

Results
The bacterial isolates reduced the growth of the black root rot causal agents Fusarium solani, Rhizoctonia solani, and Macrophomina phaseolina, in dual culture bioassays. The greatest fungal inhibition was caused by P. polymyxa isolates 1 and 2 and B. brevis isolate 2. They suppressed the growth of F. solani, R. solani, and M. phaseolina by more than 74, 76, and 79%, respectively. Disease severity and incidence were significantly reduced on naturally infected strawberry plants in the field by the six isolates. The best results were obtained by the superior bioassay isolates which suppressed the disease incidence by 73, 77, and 71%, and its severity by 72, 78, and 70%, respectively. Disease suppression by bacteria was comparable to that by fungicide Actamyl. Bacteria surpassed Actamyl with regard to strawberry yield and enhancement of peroxidase and chitinase activities in the leaves.

Conclusions
These isolates are potential benign alternatives to fungicides used against black root rot in strawberry in Egypt. More studies are needed to examine their economic use on a wider scale.
Microbiome network traits in the rumen predict average daily gain in beef cattle under different backgrounding systems
Authors: Omontese Bobwealth O. , Sharma Ashok K. , Davison Samuel , Jacobson Emily , DiConstanzo Alfredo , Webb Megan J. , Gomez Andres 
Published: March 2022

AbstractBackgroundBackgrounding (BKG), the stage between weaning and finishing, significantly impacts feedlot performance in beef cattle; however, the contributions of the rumen microbiome to this growth stage remain unexplored. A longitudinal study was designed to assess how BKG affects rumen bacterial communities and average daily gain (ADG) in beef cattle. At weaning, 38 calves were randomly assigned to three BKG systems for 55 days (d): a high roughage diet within a dry lot (DL, n = 13); annual cover crop within a strip plot (CC, n = 13); and perennial pasture vegetation within rotational paddocks (PP, n = 12), as before weaning. After BKG, all calves were placed in a feedlot for 142 d and finished with a high energy ration. Calves were weighed periodically from weaning to finishing to determine ADG. Rumen bacterial communities were profiled by collecting fluid samples via oral probe and sequencing the V4 region of the 16S rRNA bacterial gene, at weaning, during BKG and finishing.ResultsRumen bacterial communities diverged drastically among calves once they were placed in each BKG system, including sharp decreases in alpha diversity for CC and DL calves only (P < 0.001). During BKG, DL calves showed a substantial increase of Proteobacteria (Succinivibrionaceae family) (P < 0.001), which also corresponded with greater ADG (P < 0.05). At the finishing stage, Proteobacteria bloomed for all calves, with no previous alpha or beta diversity differences being retained between groups. However, at finishing, PP calves showed a compensatory ADG, particularly greater than that in calves coming from DL BKG (P = 0.02). Microbiome network traits such as lower average shortest path length, and increased neighbor connectivity, degree, number and strength of bacterial interactions between rumen bacteria better predicted ADG during BKG and finishing than variation in specific taxonomic profiles.ConclusionsBacterial co-abundance interactions, as measured by network theory approaches, better predicted growth performance in beef cattle during BKG and finishing, than the abundance of specific taxa. These findings underscore the importance of early post weaning stages as potential targets for feeding interventions that can enhance metabolic interactions between rumen bacteria, to increase productive performance in beef cattle.
Synergistic degradation of Methylene Blue by novel Fe-Co bimetallic catalyst supported on waste silica in photo-Fenton-like system
Authors: Quiton Khyle Glainmer N. , Lu Ming-Chun , Huang Yao-Hui 
Published: March 2022

AbstractIn this present study, a novel method to fabricate bimetallic Fe-Co catalyst supported on waste silica was investigated for the photo-Fenton-like (PFL) degradation of Methylene Blue (MB) dye. The uniqueness of this work is on the preparation of the catalyst via fluidized-bed crystallization (FBC) process. Under the optimum conditions of initial pH of 3.0, 3.0 mM of H2O2, and 1.0 g L-1 of FBC-derived Fe-Co/SiO2 catalyst (fFCS), the maximum response for the decoloration and mineralization efficiencies of 20 mg L-1 of MB in 60 min were 100 and 65%, respectively. Compared to the impregnated Fe-Co/SiO2 catalyst, the fFCS catalyst exhibited comparable decoloration and mineralization efficiencies, and relatively lower metal leaching for both iron and cobalt. Superoxide radical was unveiled to be the dominant reactive oxygen species in the PFL system over the fFCS catalyst. The catalysts were characterized by Fourier Transform Infrared spectroscopy, Energy Dispersive X-ray spectroscopy and Scanning Electron Microscopy. The results show the successful incorporation of iron and cobalt on the surface of the SiO2 support material.
Analysis of changes in tibial torsion angle on open-wedge high tibial osteotomy depending on the osteotomy level
Authors: Song In-Soo , Kwon Junhan 
Published: March 2022

Abstract
Purpose
This study evaluated the tibial torsional angle changes of 72 knees before and after open-wedge high tibial osteotomy (OWHTO) and compared the results according to the osteotomy level.

Materials and methods
Seventy patients (72 knees) with Kellgren–Lawrence grade 3 underwent OWHTO. Demographic data, operation procedures, and measurement of mechanical tibiofemoral angle (mTFA), anatomical tibiofemoral angle (aTFA), tibial torsional angle (TTA), and pre- and postoperative Lysholm and International Knee Documentation Committee (IKDC) scores were obtained. The authors analyzed TTA changes between 30 knees with high-level osteotomy (group A) and 42 knees with low-level osteotomy (group B).

Results
The changes of TTAs in the subjects of 72 knees went from 29.26 ± 5.6° preoperative mean to 25.36 ± 6.4° postoperative mean (p = 0.032). The postoperative TTAs of group A (mean 27.4 ± 4.8°) and B (mean 25.7 ± 4.9°) were statistically significant (p < 0.01). Preoperative Lysholm and IKDC scores of 72 knees had means of 49.1 ± 3.5 and 49.0 ± 15.2, respectively, and postoperative means of 85.7 ± 8.56 and 78.0 ± 17.6, respectively, which were statistically significant (p < 0.01).

Conclusions
Changes of TTA with internal rotation of distal tibia were observed following OWHTO. High-level osteotomy on the proximal tibia’s lateral cortex had less internal rotation of the distal tibia than low-level osteotomy.
Predictors of in-hospital mortality in diabetic patients with non-ST-elevation myocardial infarction
Authors: Ghorashi Seyyed Mojtaba , Salarifar Mojtaba , Poorhosseini Hamidreza , Sadeghian Saead , Jalali Arash , Aghajani Hassan , Haji-Zeinali Ali-Mohammad , Omidi Negar 
Published: March 2022

Abstract
Background
There have been little data about the additive effects of coronary risk factors on mortality in diabetic patients presenting with non-ST-segment elevation myocardial infarction (NSTEMI). This study aimed to evaluate the predictors of mortality in diabetic patients presenting with NSTEMI. All patients admitted to Tehran Heart Center (THC) with a confirmed diagnosis of NSTEMI and a history of diabetes mellitus (DM) type 2 between September 2003 and April 2017 were included. Clinical characteristics and paraclinical data such as lipid profiles, creatinine, hemoglobin, and hemoglobin A1C (HbA1C) were evaluated in these patients to predict in-hospital mortality. The approach for model calibration was a logistic regression with the backward elimination method.

Results
Of a total of 9158 patients with non-ST-segment elevation myocardial infarction, 3133 had diabetes mellitus type 2 and met our criteria to enter the final analysis. In the multivariable analysis, age, chronic obstructive pulmonary disease, and a severely reduced left ventricular ejection fraction (LVEF) significantly increased the rate of in-hospital mortality, whereas mildly and moderately reduced left ventricular ejection fraction did not increase the rate of mortality.

Conclusions
Age, chronic obstructive pulmonary disease (COPD), and severely reduced LVEF (< 30%) independently increased in-hospital mortality in our diabetic patients with a confirmed diagnosis of NSTEMI. Severely reduced LVEF had the strongest relationship with in-hospital mortality, whereas the mean HbA1C level and the type of DM management exerted no significant effect on in-hospital mortality.
The Significance of Mr. Richard Buckley’s Exploding Trousers: Reflections on an Aspect of Technological Change in New Zealand Dairy Farming between the World Wars
Authors: Watson James 
Published: March 2022

Abstract
During the early 1930s many New Zealand farmers enthusiastically adopted the highly inflammable chemical sodium chlorate as a weedicide against ragwort. This development reflected many aspects of New Zealand farming at the time. Dairying was expanding rapidly, reducing the control of ragwort by grazing with sheep; large areas of land cleared in the post-war boom were reverting to weeds; farmers tended to look to the state to assist with their problems and the Department of Agriculture promoted the use of sodium chlorate; farmers were generally highly literate and were rapidly aware of new possibilities; and there was a continuing shortage of labor in farming. The latter reflected New Zealand’s generally restrictive immigration policies; higher wages and greater freedom in urban employment; changing demography and attitudes to child labor; attitudes to married women working out on the farm; and, perhaps most importantly, the widespread drive for independence from farm workers and neighbors. Sodium chlorate seemed to promise a solution to the control of ragwort without the high financial and personal cost of finding additional labor.
Uncovering the Subsistence Economy in the Twentieth-Century South: Blue Ridge Mountain Farms
Authors: Gregg Sara M. 
Published: March 2022

Abstract
The small upcountry farms of Southern Appalachia were slowly fading away during the early twentieth century, but pockets of self-sufficient farms remained in places like the northern Blue Ridge Mountains of Virginia. These farmers, responsive to the constraints of the mountain landscape that surrounded them, tended to rely on less invasive, century-old technologies to work their fields. Although contemporaries widely disparaged this way of life, the records of these communities reflect a long history of viability and suggest that the ecological basis of upcountry agriculture was strong. This article, which is part of a larger project that studies the transition from small farms to publicly owned forests in Appalachia, analyzes the agricultural patterns within five Blue Ridge Mountain hollows and the economics of subsistence farming in the early twentieth century.