bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased of soybean genetic variability through hand pollination
Authors: Hanafiah D S , Haryati  , Damanik G M , Limbong M S , Joshua  , Silaen F F 
Published: July 2022

Abstract
Soybean is one of the most popular cropin Indonesia. In the last five years, soybean production has decreased. Therefore, one way to increase the productivity and genetic variability of soybean is hand pollination. This study aims to see and observe the flower and the fertility of soybean pollen and to know the correct stages of crossing. This study was conducted by observing the morphology and physiology of Grobogan and Anjasmoro soybeans. Parameters observed were flower parts, crossing stage, and pollen fertility. The result of this study showed that Grobogan and Anjasmoro were monoecious. In the fertility test, several samples found that Grobogan and Anjasmoro flowers were 100% fertile. In performing hand pollination, there are several stages, as follows; the selection of male and female flowers, castration, emasculation, pollination, isolation, and labeling. Crosses of varieties between Grobogan and Anjasmoro have good enough success rate, which is equal 57.14%.
Molecular docking analysis of Allium chinense compounds as Secreted Aspartyl Proteinase-5 (SAP5) inhibitor
Authors: Hartanto A , Naibaho F G , Panjaitan D , Lutfia A , Munir E 
Published: July 2022

Abstract
Secreted Aspartyl Proteinase-5 (SAP5) or candidapepsin-5 is known as the current and major virulence factor in the biofilm formation of Candida albicans. The protein is secreted into the environment to disrupt the host immune cells and degrade keratin then penetrating the host defense to express its pathogenicity. SAPs has been targeted for many studies including in vitro test and in silico analysis of potential inhibitory agents. In the current study, we tested six selected compounds in the aqueous extract of Allium chinense G. Don. namely 1-tetradecanol, anozol, hyacinthin, isosorbide, mannitan and oleic acid for in silico analysis along with pepstatin A as the most potent inhibitor or control. The results obtained that oleic acid displayed the most stable bonding with the SAP5 based on molecular docking, visualization and data analysis although slightly lower than anozol in terms of binding affinity. Oleic acid also produced the most similar number of binding residues with pepstatin A based on 2D feature with also similar region in the pocket of SAP5 based on 3D visualization. Hence, the compound may be potentially developed as leading compound in treating C. albicans infections.
Exploring endophytic fungi from Rhizophora spp leaves for improving plant growth
Authors: Elfiati D , Delvian  , Syafitri M , Hidayat A 
Published: July 2022

Abstract
Endophytic fungi are a group of fungi inhabiting the plant tissue for part or all of their life cycle without exposing any hazard to the host. Endophytic fungi can produce bioactive chemicals or secondary metabolites in proportion to their host plant, therefore no parts of the plant are needed to obtain these compounds. As a result, the survivability of a plant survival is maintained. The aim of this study was to obtain endophytic fungi isolated from the leaves of Rhizophora mucronata and Rhizophora apiculata. R. mucronata and R. apiculata leaves were collected at random in Lubuk Kertang Village, West Brandan District, Langkat Regency, North Sumatra. Endophytic fungal isolation was conducted on potato dextrose agar (PDA) medium. The fungal isolates were morphologically identified to the genera level. In this study, 8 isolates from R. mucronata leaves and 9 isolates from R. apiculata leaves were obtained. According to morphological identifications, a total of 8 endophytic fungal isolates from R. mucronata leaves belonged to the genus Aspergillus, while 9 isolates from R. apiculata leaves belonged to three genera: Aspergillus (7 isolates), Cunninghamella (1 isolate), and Humicola (1 isolate).
Adaptation of Physalis angulata L. to salinity stress as an environmental factor in terms of morphological response
Authors: Sholehah D N , Hariyanto S , Purnobasuki H 
Published: July 2022

Abstract
Salinity-affected areas include dry and semi-arid parts of the world, with low crop production capacity. P. angulata is economically important of genus Physalis due to its nutraceutical benefit. This study aims to evaluate the morphological response of P. angulata to salinity stress. The research was conducted in Bangkalan Madura. Salinity treatments were consist of 0, 20, 40, 60, 80, 100, 120, 140, 160, 180. Research has been designed according to randomized block design with three replications. Each replication consists of 4 plants. Leaf morphology was observed by measuring the chlorophyll content in young leaves and visual symptoms in old leaves. The response of plant roots to salinity was carried out by measuring root volume and primary root length. The study result that morphological changes occur in the leaves and roots. There is an acceleration of senescence of old leaves and a decrease in young leaves chlorophyll. The root changes can be seen from the root volume and primary root length reduction. The changes began to occur at a concentration of 80 mM except for the primary root length, which begins to appear at a concentration of 120 mM.
Application of Bacillus cereus Biofertilizer Formulation of Soybean (Glycine max L. Merril) Growth and Yield Support Sustainable Agriculture on Peatlands
Authors: Hapsoh  , Dini I R , Wawan  , Wulandari M 
Published: July 2022

Abstract
Soybean growth and yield can only be increased by giving biofertilizers containing Bacillus cereus. Several formulations of organic matter as carriers are expected to increase the effectiveness of B. cereus in biofertilizer. Therefore, this research aims to determine the effect of various formulations of B. cereus biofertilizer on the growth and yield of soybean (Glycine max L. Merril) on peat medium. The experimental research design used a Completely Randomized Design (CRD) with 5 treatments and 4 replications (20 experimental units) with the treatment tested was the formulation of B. cereus (F): 100 ml of B. cereus without formulation (F0); 100 ml of B. cereus + 74% bagasse + 13 % zeolite + 13% dolomite (F1); 100 ml of B. cereus + 74% rice husk + 13% zeolite + 13% dolomite (F2); 100 ml of B. cereus + 74% solid + 13% zeolite +13% dolomite (F3); 100 ml of B. cereus + 74% OPEFB + 13% zeolite + 13% dolomite (F4). The results showed that the application of all organic materials in the formulation had not been optimally able to increase every parameter of growth and production. However, the B. cereus + oil palm empty bunches (OPEFB) tended to be better for the percentage of effective root nodules and seed weight per 3 soybean plants.
Virtual screening of antidiabetic compounds from common-urban herbs in Indonesia based on alpha-amylase inhibition
Authors: Fauziyah  , Lukitasari M , Rahmasuha S , Jadid N , Ramadhan R , Hidayati D 
Published: July 2022

Abstract
The common-urban herb (c-uh) plants in Indonesia were known as TOGA plants that can use as traditional medicines hence can improve the quality of health and the economy. Since the diabetic case in the city is usually high prevalence, the investigation of c-uh potential for diabetic medicines are strategic due to its accessibility. Recently, the activity of herbal bioactive compounds for medical purposes can be screened prior to in vitro or in vivo tests, including for diabetic medicine. One of the diabetic treatments is conducted by decreasing glucose absorption in the intestine via alpha-amylase inhibition. This research was aimed to obtain the potential of bioactive compounds from six Indonesian c-uhs (Imperata cylindrica, Cassia siamea, Apium graveolens, Catharanthus roseus, Physalis angulata, and Peperomia pellucida) using the molecular docking method. The bioactive compounds were selected referred to several references, i.e Cylindrene, Dihydroquercetin, Limonene, Lochnerine, Vindolidine, Withangulatin-A, and Yohimbine which were used as ligands. The 3D of ligands were obtained from the PubChem and then screened virtually of each binding affinity toward the alpha-amylase using PyRx method. The results showed that Withangulatin-A from Physalis angulata has the highest potential among other candidates for bioactive compounds as alpha-amylase inhibitors for antidiabetic treatment.
The timing of sonic bloom application on cabbage (Brassica oleraceae) for foliar fertilizer effectiveness
Authors: Mawarni L , Lahay R R , Fajari A 
Published: July 2022

AbstractSonic bloom was a technology that used sound waves at 07.00–11.00 a.m. in the western part of Indonesia with a particular frequency to stimulate plant growth. This study aims to determine the effectiveness and best time for applying the foliar fertilizer with sound waves to cabbage plants. This research was conducted in Kuta Gugung Village, Naman Teran District, Karo Regency, from September 1st, 2020, to December 9th, 2020. The research method was a non-factorial randomized block design with four treatments and five replications, namely: application sound waves without fertilization (control), fertilization at 1 hour before application sound waves, fertilization carried out simultaneously with application sound waves, and fertilization carried out 1 hour after application sound waves. The results showed that the application of foliar fertilizer with sound waves significantly affected stomata exposure, making fertilizer more effective. To get the best stomata exposure, it was at 8.30 a.m., but the average selling weight of the crop was not significant.
The effect of manure and ascorbic acid to the growth of Vetiveria zizanioides L. in saline soil
Authors: Novita A , Widodo S W , Madjid M , Mariana M , Nora S , Basri A H H 
Published: July 2022

Abstract

Vetiveria zizanioides was known as vetiver that produces a good quality oil and is also competent in foreign markets. This study aims to determine the effect of manure and ascorbic acid on the growth of vetiver on saline soil by applying the appropriate dose of manure and ascorbic acid for optimal growth. The methodology was done using a randomized block design with three replicates and consisted of 2 factors, i.e., manure level (K) and the ascorbic acid concentration (A). The results suggested an interaction of the manure and ascorbic acid concentration in the plant height, root volume but not found in the number of clumps. The manure displays a significant response to plant height, number of leaves, number of clumps, and root volume. The ascorbic acid response to the growth was obtained to the best response in plant height, a number of leaves, and root volume. The dose of manure and ascorbic acid that increased concentration was suspected to control salinity stress. The manure was thought to be able to provide the organic matter in saline soil because the addition of organic matter holds water and retains nutrients. Besides, ascorbic acid has been a role antioxidant during plant stress.
Local wisdom on processing and utilization of geronggang’s oil for daily remedy and healthcare
Authors: Latifah H , Akbar O T , Irawan A , Kholibrina C R , Aswandi A 
Published: July 2022

Abstract
Geronggang oil has been utilized in various traditional remedy for a long time. However, oil processing from Cratoxylon arborescens applied in simple technique through boiling water, while extraction involving organic solvents to increase productivity were limited. This study aims to explore local wisdom in processing of geronggang oil through applications of several fatty oils such as virgin coconut oil; virgin olive oil; moringa oil; and castor oil and to explore the local knowledge on traditional utilization and sustainable management of this non-timber forest product. Samples of dried bark with 20% moisture content from 8-year-old plants were involved in study. Some proportions of weight of barks were immersed in particular fatty oils and then heated at 100°C. Various literatures were reviewed regarding the traditional application of this organic oil. The results showed that all carrier oils dissolved the extractive substances contained in the barks. The colour of solution becomes darker in higher proportion of barks to carrier oil. This indicates the higher concentration of oil-soluble extractives. It required 30 minutes to dissolve most of extractives, and no colour change was observed thereafter. Furthermore, local wisdom revealed the potential of geronggang oil for various daily remedies and for skin care products.
Characteristics of Bromelain enzyme from Queen variety pineapple crown at different drying temperatures
Authors: Susanti S , Rizqiati H , Pratama Y , Arifan F , Reza S P 
Published: July 2022

Abstract
Pineapple (Ananas comosus (L) Merr) is a plant that contains bromelain enzymes in almost all parts of the plant, such as fruit, leaves, crown, and the stem with different amounts. Bromelain is included in the protease enzyme group that can break down the molecular structure of proteins into amino acids. Pineapple crown is one of the waste products from pineapple processing that has not been used optimally. This study aims to utilise Queen pineapple crown waste and determine the characteristics of Queen pineapple crown bromelain enzymes which consist of protein content, unit activity, and specific activity at different drying temperatures. Queen pineapple crowns were dried using a cabinet dryer at the temperature of 35°C, 40°C, 45°C, and 50°C before the extraction process. The optimum temperature in the Queen pineapple crowns drying process to produce bromelain with a protein content of 4.41 mg/ml, unit activity 1.36 U/ml, and specific activity 0.31 U/ml was 40°C, then purified by adding 20%, 40%, 60%, and 80% ammonium sulfate. The optimum concentration of ammonium sulfate used to produce pure bromelain (0.33 U/mg specific activity) from Queen pineapple crowns was 60%.
Grafting compatibility between variants of sandalwood (Santalum album Linn.) in Gunungkidul, Indonesia
Authors: Prastyo B , Indrioko S , Faridah E , Ratnaningrum Y W N 
Published: July 2022

AbstractThe declining number of adult individuals led to changes in the mating system of certain sandalwood (Santalum albumLinn.) populations previously dominated by outcrossing. The genetic quality of the produced seeds and reproduction success decreased due to inbreeding. Therefore, vegetative propagation by grafting is an alternative method to support sandalwood conservation and development. This study aimed to determine the compatibility of rootstocks and scion for vegetative propagation by top cleft grafting on sandalwood variants found in Petir and Wanagama landraces, Gunungkidul Regency, Indonesia. Based on the availability of materials, we used Red Big Flower (RBF), Red Small Flower (RSF), and Yellow Small Flower (YSF) variants, with RBF and RSF as rootstocks and RBF, RSF, and YSF as scions. All six combinations of rootstock-scion indicated compatibility with various results. The average grafting survival was 35.8%, and the RBF-YSF combination showed the highest survival (90%). However, the RSF-YSF combination produced leaves on the scion at most (21 blades). The propagation method of grafting can be applied for the conservation and deployment of sandalwood variants. In tree improvement, grafting is essential to set up the stool plant in a hedge orchard, copy the genetic materials for the clonal test and clonal seed orchard.
The response of paddy with biochar application and legowo planting system at Tanjung Garbus Kampung Village
Authors: Sipayung A , Damanik R I 
Published: July 2022

Abstract
A black charcoal from the heating of biomass with limited or without oxygen called Biochar. It is organic matter that has stable charateristics could be removed in dry land. The purpose of this study was to evaluate the response of biochar application on paddy fields and legowo planting systems. The Factorial Plan Split Plot Design was used for this research with two factors, namely; the treatment of planting system (main plot/S) with two stages, Jajar Legowo 2:1 and Jajar Legowo 4:1 and biochar’s application as sub-plot with three level; control, 25.5 ton/ha, and 50.5 ton/ha. The research was carried out in Tanjung Garbus Kampung’s Village, Pagar Merbau Sub-district, Deli Serdang Regency with an elevation of ± 50 Meters above sea level. The paddy field has irrigation system for watering the crops. The biochar application and legowo planting system showed a slight significant effect on the root volume. However, the application of biochar and legowo planting systems statistically unaffected on the weight of 1000 grains of paddy crops. Therefore, legowo planting system had slight difference on the production per hectare for paddy.
Genetic diversity analysis of several maize lines (Zea mays L.) developed in acid soil using molecular markers RAPD
Authors: Lubis K , Butarbutar L K S 
Published: July 2022

Abstract
Abstact. Maize is the second staple crop in Indonesia with optimal temperature between 21-34 °C, soil pH between 5.6-7.5 and altitude between 1000-1800 m above sea level. Efforts to increase the potential of marginal soils really need to be done to support food needs in Indonesia, one of the ways to be done is by increasing acid stress-resistant maize varieties in Indonesia. This research was conducted at the Molecular Genetics Laboratory of the Faculty of Agriculture University of North Sumatra, Medan from March-July 2021. Determination of band size using the UVITEC Cambridge FireReader software and the calculation of genetic similarities and group formation using the MVSP software (ver. 3.2). Based on 5 plant samples from varietis CIMMYT and Maros then isolated using primers OPC 08, OPD 05 and OPA 03. Based on the results of phylogenetics with UPGMA, all analyzed samples were divided into 2 groups. Group I consistsof CLA 84 × NEI 9008, CLA 84 and NEI 9008. The second group II consisted of CLA 106 and 1027.13 × NEI 9008
The intercropping soybean searches in The National Center for Biotechnology Information (NCBI)
Authors: Wagino  , Hanum C , Rahmanta  
Published: July 2022

Abstract
The increasing soybean production is constrained by land area, so alternative cultivation is needed through an intercropping system, including sustainable use of oil palm plantations. This study aims to obtain online information at the National Center for Biotechnology Information (NCBI)”to access soybean intercropping information regarding the literature on biological information data, the PubMed database, health, genomes, genes, and proteins. Reportedly there are: 1 book and report; 159 online books; 576 DNA and RNA nucleotides reported;”and 969 full-text journal articles on intercropping soybean cultivation. Soybean intercropping in biotechnology research provides information about research on intercropping, including soybean-oil palm integration in oil palm plantations that has not been reported inNCBI.
The role of agricultural extension on the productivity of arabica coffee farmers’ (Study: Sait Buttu Saribu, Pematang Sidamanik District, Simalungun Regency)
Authors: Lubis S N , Wardanti S 
Published: July 2022

Abstract
The purpose of this research is to determine the role of agricultural extension on the productivity Arabica coffee farmers and to find out the obstacles faced by agricultural extension in providing information concerning the productivity of Arabica coffee farmers in Sait Buttu Saribu Village, Pematang Sidamanik District, Simalungun Regency. The method of determining the sample in this study used simple random sampling with the research sample were farmers who participated in counseling activities and using to 38 samples. Data analysis was carried out by means of Likert scale and descriptive analysis. The results showed that the role of agricultural extension workers as dynamists and facilitators was in the category of important role, while the role of counselor workers as motivators was in the category of very important role and the constraints faced by agriculture counselor in extension activities were that lack of farmers participate in couseling activities and there are inadaquate facilities and infrastructure.
Analysis of rice supply determinants in North Sumatera
Authors: Lindawati  , Emalisa  , Zulfida I 
Published: July 2022

Abstract
The problem of rice supply is a continuous problem that continues to affect food security in North Sumatra every year. This study aims to find out the factors related to the problem of rice supply and what factors have a significant effect on rice supply. The study was conducted in North Sumatera with the consideration of a decrease in production that could interfere with food security. The results showed that several factors had a major influence on rice supply, including rice prices, rice production, rice consumption, rice imports, government rice reserves, land conversion, prices of production factors such as fertilizers, land conversion and food diversification. Some variables that have a significant effect on rice supply include North Sumatera Rice Price (NSRP), North Sumatera Harvest Area (NSHA) and North Sumatera Fertilizer Price (NSFP). Two variables that have a positive effect are NSHA and NSFP, while one variable has a negative effect, namely NSRP. Of the five variables, there are two variables that have no significant effect, namely North Sumatera Rice Consumption (NSRC), North Sumatera Cassava Price (NSCP).
Factors related to decisions of farmers victims of the eruption choosing temporary work as an effort for sustainability of livelihoods in refugee locations (case: refugee farmers victim of the Mount Sinabung eruption from Guru Kinayan, Gamber, Kuta Tonggal and Beras Tepu Village, Karo Regency)
Authors: Maryunianta Y , Kesuma S I 
Published: July 2022

Abstract
This study was carried out with the aim of identifying the type of temporary work chosen by the eruption victim farmers as an effort for sustainability of livelihoods in refugee locations, analysing various factors related to the decision of the eruption victim farmer to choose temporary work during the evacuation, and comparing the family income of the eruption victim farmer after with before evacuating. The sample size was determined using the Slovin method so that a total sample of 63 farmers was obtained. Sampling was done by simple random sampling method. To examine factors related to the decision of eruption victim farmer to choose the type of temporary work, the Chi Square Method was used. Results of the research are the type of temporary work for the eruption victim farmers during the evacuation are farm workers, farmers and non-agricultural workers (i.e. construction workers, drivers, small merchants); socio-economic factors related to the decision of farmers to choose the type of temporary work during the evacuation are additional skills, family dependent, intensity of attending traditional events, and temporary farmer’s residence status; there a significant decrease in the income of the families of eruption victim farmer between after and before evacuating.