bluuebunny/crossref_metadata_2025_split huggingface dataset:
Driver mutations in GNAQ and GNA11 genes as potential targets for precision immunotherapy in uveal melanoma patients
Authors: García-Mulero Sandra , Fornelino Roberto , Punta Marco , Lise Stefano , Varela Mar , Moreno Rafael , Costa-Garcia Marcel , Gros Alena , Alemany Ramón , María Piulats Josep , Sanz-Pamplona Rebeca 
Published: September 2022

AbstractUveal melanoma (UM) is the most common ocular malignancy in adults. Nearly 95% of UM patients carry the mutually exclusive mutations in the homologous genes GNAQ (aminoacid change Q209L/Q209P) and GNA11 (aminoacid change Q209L). UM is located in an immunosuppressed organ and do not suffer immunoediting. Therefore, we hypothesize that driver mutations in GNAQ/11 genes could be recognized by the immune system. Genomic and transcriptomic data for primary uveal tumors was collected from TCGA-UM dataset (n=80). The immunogenic potential for GNAQ/GNA11 Q209L/Q209P mutations was assessed using a variety of tools and HLA types information. The immune microenvironment was characterized using gene expression data. All prediction tools showed stronger GNAQ/11 Q209L binding to HLA. The immunogenicity analysis revealed that Q209L is likely to be presented by more than 73% of individuals in 1000G database whereas Q209P is only predicted to be presented in 24% of individuals. GNAQ/11 Q209L showed higher likelihood to be presented by HLA-I molecules than almost all driver mutations analyzed. Samples carrying Q209L had a higher immune-reactive phenotype: (i) expression of antigen presenting genes HLA-A (p=0.009) and B2M (p=0.043); (ii) immunophenoscore (p=0.008); (iii) infiltration of immune system cells NK (p=0.002) and CD8+ T lymphocytes (p=0.02). Results suggest a high potential immunogenicity of the GNAQ/11 Q209L variant that could allow the generation of novel therapeutic tools to treat UM like neoantigen vaccinations.
Long-ranged formation of the Bicoid gradient requires multiple dynamic modes that spatially vary across the embryo
Authors: Athilingam Thamarailingam , Nelanuthala Ashwin V.S. , Breen Catriona , Wohland Thorsten , Saunders Timothy E. 
Published: September 2022

AbstractMorphogen gradients provide essential positional information to gene networks through their spatially heterogeneous distribution. Yet, how morphogen gradients form is still hotly contested, with multiple models proposed for different systems. Here, we focus on the transcription factor Bicoid (Bcd), a morphogen that forms an exponential gradient across the anterior-posterior (AP) axis of the earlyDrosophilaembryo. We utilise fluorescence correlation spectroscopy (FCS) and perturbations to Bcd, to dissect Bcd dynamics at multiple spatial and temporal locations. In both the cytoplasm and nucleus, we find two dynamic modes for Bicoid diffusion dynamics, consisting of fast and slow populations of Bcd. Surprisingly, there are spatial differences in Bcd diffusivity along the AP-axis, with Bcd diffusing more rapidly in the posterior. We establish that such spatially varying differences in the Bcd dynamics are sufficient to explain how Bcd can have a steep exponential gradient in the anterior half of the embryo and yet still have an observable fraction of Bcd near the posterior pole. We subsequently investigated which binding elements of Bcd are playing a role in its dynamics. In the nucleus, we demonstrate that the slower mode of Bcd transport is due to Bcd DNA binding. Addition of the Bcd homeodomain to eGFP::NLS can qualitatively replicate the observed Bcd concentration profile, suggesting this domain is the primary region regulating Bcd dynamics. This study provides a detailed analysis of morphogen dynamics at different spatial and temporal locations, revealing multiple modes of transport. These results explain how a long-ranged gradient can form while retaining a steep profile through much of its range.
Has the fever left a burn?A study of the impact of COVID-19 remote working arrangements on public accountants’ burnout
Authors: Bakarich Kathleen M. , Marcy Amanda S. , O’Brien Patrick E. 
Published: September 2022

Purpose
This study aims to investigate the effects of COVID-19 working arrangements on role stress, burnout and turnover intentions in public accounting professionals. Additionally, while all professionals have had to adapt to this rapid change in working environment, this paper explores whether the impact of this transition differs depending on demographic factors, namely, rank, gender, firm size and service line.

Design/methodology/approach
The authors survey 159 public accountants working in audit and tax on their perceptions of role stress, burnout and turnover intentions before COVID-19 and since. The survey used validated instruments from prior literature to capture these measures.

Findings
Results show that role stress, burnout and turnover intentions increased significantly since remote work began. Specifically, the rank of accountants significantly affects this association, with staff experiencing the most significant increases in role stress and burnout and seniors reporting significantly higher intentions to leave their current firms. Females experience a significant increase in feelings of emotional exhaustion and turnover intention, while males experience a significant increase in feelings of depersonalization and role overload. Finally, there is a positive association between firm size and burnout, with employees from national/midsize firms experiencing the largest increase in feelings of emotional exhaustion, reduced personal accomplishment and depersonalization.

Originality/value
Given that all prior research on role stressors, burnout tendencies and turnover intentions in the context of public accounting was conducted in the pre-COVID-19 work environment, this paper examines a timely and significant event that is likely to have a long-lasting impact on the way in which people work. As accounting firms seek to develop new working models and promote well-being among their employees, they should take note of the findings of this study that gender, rank and firm size result in differential impacts on role stress, burnout and turnover intentions.
Platform-centric vs. multi-party service failure: an examination of consumers’ negative word of mouth about sharing economy platforms
Authors: Wu Laurie , Liu Stephanie Q. , Ma Shihan (David) , Hanks Lydia 
Published: September 2022

Purpose
This paper aims to identify platform-centric versus multiparty service failure on sharing economy platforms via topic modeling analysis of consumers’ negative online reviews. The authors also sought to understand consumers’ reactions to these experiences by detecting negative discrete emotions. The authors then contrasted consumers’ responses to platform-centric and multiparty service failure through the theoretical lens of failure controllability.

Design/methodology/approach
The authors used a large-scale data set containing more than 81,000 negative app reviews on eight representative hospitality and tourism sharing economy platforms. Topic modeling coupled with emotion detection algorithms revealed 11 themes reflecting diverse forms of platform-centric versus multiparty service failure and their associations with negative discrete emotions based on regression analysis.

Findings
The 11 themes reflecting diverse forms of platform-centric versus multiparty service failure were as follows: app glitch, customer service, locating and pooling, account issues, transaction, offer redemption, interface challenges, intermediary inaction, service lateness and cancellation, incorrect order and fee structure. The analysis suggests that platform-centric service failure is more likely than multiparty service failure to elicit negative discrete emotions.

Originality/value
The research enriches the understanding of platform-related service failure beyond dyadic service interaction. In particular, the authors bring to light two forms of platform-related service failure that warrant scholarly attention: platform-centric versus multiparty service failure. By uncovering the distinct negative emotional associations of platform-centric versus multiparty service failure, the research adds novel empirical evidence to the service failure literature and the relevant attribution theory. Findings offer long-term implications for the sustainable development of sharing economies and platform businesses in contemporary hospitality.
Growth implications of creation and discovery behavior among family firms: the moderating role of venture age
Authors: Donbesuur Francis , Hultman Magnus , Boso Nathaniel , Oghazi Pejvak 
Published: September 2022

PurposeThe aim of the study is to examine the effects of opportunity creation and discovery on the performance of family firms. Specifically, from the tenets of dynamic capabilities and organizational contingency perspectives, this study proposes and tests a framework of how family firms' creation and discovery behavior impact venture growth and the conditions under which such impact can vary.Design/methodology/approachThe study uses moderated-hierarchical regression to analyze survey data from 156 family-owned small and medium-sized enterprises (SMEs) operating within a sub-Saharan African economy.FindingsThe findings indicate that creation behavior has a curvilinear U-shaped relationship with venture growth, while discovery behavior has a direct positive relationship with venture growth. Further analysis reveals that the curvilinearity of the U-shaped relationship between creation and venture growth will be stronger for older family firms than for younger ones.Research limitations/implicationsThe study findings may be limited by the cross-sectional nature of the data and the specific focus on family firms only.Practical implicationsThe results highlight the significance of pursuing both opportunities among family firms. In fact, both creation and discovery opportunities are significant drivers of family firm growth, albeit in different capacities. Relatedly, managers of older family firms (compared to younger firms) can invest more in exploiting creative opportunities.Social implicationsFrom these findings, governments and other stakeholders should create enabling environment and institutional frameworks conducive to exploiting opportunities by entrepreneurial firms.Originality/valueThe study is novel – as it provides unique findings on the performance implications of creation and discovery behavior of entrepreneurial family firms within developing economies.
Patient engagement behaviors in hospitals: the role of word of mouth and patient helping behaviors
Authors: Fattahi Majid , Farzin Milad , Sadeghi Marzie , Makvandi Rosha 
Published: September 2022

Purpose
The purpose of this study is to investigate patient perceived value as a stimulus of patient engagement behaviors both from the conceptual and empirical perspectives.

Design/methodology/approach
Based on the stimulus–organism–response framework, the authors developed a model to determine the impact of patient perceived value on patient engagement behavior in health care. The data were collected from a sample of 391 patients hospitalized in private hospitals. Structural equation modeling technique was used to test the research hypotheses.

Findings
The findings confirmed relevance of the service quality dimensions reliability, tangibility, responsiveness and empathy as significant antecedents of patient perceived value. Perceived value plays a significant role in shaping word of mouth and patient helping behaviors.

Research limitations/implications
The findings of this study are relevant and applicable to patients in private hospitals.

Practical implications
This study contributes to the literature by providing new evidence on patient perceived value and engagement behaviors as a response to care quality. With adequate focus on perceived value and service quality, service providers can strengthen the relationship with patients and build a sustainable competitive advantage, by stimulating engagement behaviors in patients.

Originality/value
This study is of unique value to the health-care literature, both from the theoretical and managerial point of views. This study proposes a conceptual model of patient perceived value which can be used in the private health sector. Moreover, this study contributes to the health-care literature by introducing patient-helping behavior.
Social exchange in buyer-supplier relationships and innovation speed: the mediating and moderating role of information sharing and knowledge channels
Authors: Ojha Divesh , Dayan Mumin , Struckell Beth , Dhir Amandeep , Pohlen Terrence 
Published: September 2022

Purpose
This study recognizes service as the majority contributor to global and US gross domestic product and the importance of innovation speed to service innovation. Generating innovative products and services at a faster rate generates advantages for business-to-business (B2B) service organizations in keeping up with and moving ahead of rivals. This study aims to introduce the concept of capacity for social exchange (CSE) in buyer–supplier relationships, which reflects the degree to which individuals possess competencies that enable the exchange of information, and this study also explores how CSE affects knowledge sharing and innovation speed within a supply chain organization.

Design/methodology/approach
The sampling frame of this research consisted of service businesses in the USA. The data were collected through Zoomerang, an online survey research firm where the B2B panel of Zoomerang formed the sampling frame. The data was collected from 264 B2B service sector executives.

Findings
The key findings are as follows: CSE facilitates knowledge sharing; knowledge sharing is positively related to innovation speed; and the relationship between CSE and innovation speed is fully mediated by knowledge sharing. Additional analysis reveals that knowledge channels which are designed as boundary-spanning strategies used to transfer and create knowledge between business units, directly increase – and positively moderate – the relationship between CSE and innovation speed.

Originality/value
This study addresses the gap in literature focused on micro-level influencers on innovation. This study sets out by presenting the concept of CSE, and this study addresses limitations in prior work by examining the research questions.
URBAN NOSTALGIA, REGENERATION, AND CULTURAL MONOPOLY RENT: ‘Nanjing 1912’ and Its Branding Bubbles
Authors: Lan Cassidy I‐Chih , Chen Jianglong 
Published: September 2022

AbstractNostalgia has become an important aspect of culture‐led urban regeneration in advanced cities. Chinese cities have also experienced this tendency because many local states have used nostalgia as an instrument to brand their cities and promote business opportunities. However, culture‐led regeneration has resulted in the dilemma of cultural commodification and branding bubbles, as this approach is subordinate to capital circulation and property revitalization. We translate the Marxist idea of monopoly rent proposed by David Harvey into culture‐led regeneration by focusing on the influence of urban nostalgia on the production of the built environment and place distinctiveness for capital accumulation. Against the background of national capital in the Republic of China and its current fervor for culture‐led regeneration in what is known as the ‘Republican style’, in this article we explore the case of Nanjing 1912 to examine how urban cultural monopoly rent is produced through the project, and scrutinize its dilemma of over‐replication. Although nostalgia is associated with culture, creativity and history, Nanjing 1912's regeneration is underpinned by economic revenue and by investors experiencing branding bubbles as they tried to upscale the 1912 brand to Nanjing and its neighboring cities.
Effect of race on the measurement of angiogenic factors for prediction and diagnosis of pre‐eclampsia
Authors: Wright Alan , von Dadelszen Peter , Magee Laura A. , Syngelaki Argyro , Akolekar Ranjit , Wright Dave , Nicolaides Kypros H. 
Published: September 2022

AbstractObjectiveTo examine the effect of self‐declared race on serum placental growth factor (PlGF) and sFlt‐1/PlGF ratio and the impact on pre‐eclampsia (PE) prediction.DesignProspective observational study.SettingTwo UK maternity hospitals.Population29 035 women with singleton pregnancies attending a routine 35+0 to 36+6 weeks' gestation hospital visit, including 654 (2.3%) who subsequently developed PE.MethodsThe predictive performance of PlGF and sFlt‐1/PlGF for PE in minority racial groups (versus white) was examined.Main outcome measureDelivery with PE.ResultsCompared with white women, mean PlGF was higher and sFlt‐1/PlGF ratio lower in black, South Asian, East Asian and mixed race women. In white women at a PlGF concentration cut‐off corresponding to a screen‐positive rate (SPR) of 10%, detection rates (DRs) were 49.1% for PE at any time and 72.3% for PE within 2 weeks after screening. In black women, at the same PlGF concentration cut‐off for white women, the SPR was 5.5%, and DRs 33.6% and 55.0%, respectively; the number of PE cases was too small to evaluate screening performance in other racial groups. Using a fixed cut‐off in sFlt‐1/PlGF ratio to identify women at risk of developing PE, similarly diagnostically disadvantaged black women. Bias was overcome by adjusting metabolite concentrations for maternal characteristics and use of the competing risks model to estimate patient‐specific risks.ConclusionScreening for PE with fixed cut‐offs in PlGF or sFlt‐1/PlGF diagnostically disadvantages black women. It is essential that measured levels of PlGF be adjusted for race as well as other maternal characteristics.
Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing
Authors: Re Angela , Mazzoli Roberto 
Published: September 2022

AbstractIn the last decades, fermentative production of n‐butanol has regained substantial interest mainly owing to its use as drop‐in‐fuel. The use of lignocellulose as an alternative to traditional acetone–butanol–ethanol fermentation feedstocks (starchy biomass and molasses) can significantly increase the economic competitiveness of biobutanol over production from non‐renewable sources (petroleum). However, the low cost of lignocellulose is offset by its high recalcitrance to biodegradation which generally requires chemical‐physical pre‐treatment and multiple bioreactor‐based processes. The development of consolidated processing (i.e., single‐pot fermentation) can dramatically reduce lignocellulose fermentation costs and promote its industrial application. Here, strategies for developing microbial strains and consortia that feature both efficient (hemi)cellulose depolymerization and butanol production will be depicted, that is, rational metabolic engineering of native (hemi)cellulolytic or native butanol‐producing or other suitable microorganisms; protoplast fusion of (hemi)cellulolytic and butanol‐producing strains; and co‐culture of (hemi)cellulolytic and butanol‐producing microbes. Irrespective of the fermentation feedstock, biobutanol production is inherently limited by the severe toxicity of this solvent that challenges process economic viability. Hence, an overview of strategies for developing butanol hypertolerant strains will be provided.
An ethical assessment of the use of old and new methods to study sociality in wild animals
Authors: Gruber Thibaud 
Published: September 2022

Abstract

The use of novel tools and technologies is transforming our approaches to study wild animal behaviour. Yet, from disturbance of natural habitats through field experiments to tagging, there is much ground for researchers to provide clear ethical assessment of their research practices and how they can affect wild animals.

In this perspective, I review the current trends in field animal behaviour research, with a focus on studies on sociality, and investigate the forms under which such practices can impact animals. I focus particularly on the notions of disturbance and invasiveness.

I build a parallel with the legal classifications in terms of degrees of severity found in the Swiss law and aimed at protecting animal well‐being, inspired by the principles of the 3R (Replace, Refine, Reduce) used in invasive animal research.

I highlight how the impact of research can be assessed and monitored, particularly through behavioural and physiological means, identifying areas where such monitoring is critical, but also acknowledging the theoretical and practical limitations to such tools.

I hope to trigger discussions on an ethical use of new tools and technologies, which can both inspire new research while limiting the disturbance to wild animals.
Rates and predictors of mortality of very low birthweight infants in three Nigerian tertiary hospitals
Authors: Fajolu Iretiola B. , Mairami Amsa B. , Okonkwo Ikechukwo , Ezenwa Beatrice , Otuneye Adekunle T. , Amuabunos Emmanuel A. , Mukhtar‐Yola Mariya , Audu Lamidi I. , Ezeaka Veronica C. , Ekhaguere Osayame A. 
Published: September 2022

AbstractAimWe aimed to describe in‐hospital mortality, and its predictors, in very low birthweight (VLBW) infants managed in tertiary centres in a low‐ to middle‐income country.MethodsThis was a retrospective cohort study of VLBW infants (birthweight 500 to 1500 grams) admitted within 72 h of life to the neonatal intensive care units (NICUs) of three tertiary centres in Nigeria from July 2017 to March 2021. We describe in‐hospital mortality rates, causes and when they died. The independent predictors of in‐hospital mortality were determined using multivariate logistic analysis.ResultsOf the 6187 NICU admissions, 1161 met the inclusion criteria: 545 (47%) VLBW infants died, including 309 (57%) from respiratory distress syndrome, and 55% occurred within 72 h of life. The adjusted odds (aOR) for mortality increased with each extra Downes respiratory distress score (aOR 1.27) with a 95% confidence interval (CI) of 1.14–1.41. Study site 3 had a higher aOR for mortality than site 1 (aOR 2.78, 95% CI 1.72–4.48) and site 2 (aOR 2.29, 95% CI 1.45–3.61).ConclusionNearly half (47%) of all VLBW infants admitted to three tertiary referral hospitals in Nigeria died during hospitalisation. Mortality varied significantly by site and both the centre and respiratory distress independently predicted mortality.
Preventive interventions for children of parents with depression, anxiety, or bipolar disorder: A quasi‐experimental clinical trial
Authors: Wirehag Nordh Emme‐Lina , Grip Karin , Thorvaldsson Valgeir , Priebe Gisela , Afzelius Maria , Axberg Ulf 
Published: September 2022

AbstractAimTo investigate the effectiveness of preventive interventions for 8–17‐year‐old children of patients diagnosed with depression, anxiety, or bipolar disorder.MethodsSixty‐two families including 89 children received either the more extensive Family Talk Intervention (FTI; n = 35), the brief Let's Talk about Children (LTC; n = 16), or Interventions as Usual (IAU; n = 38) in routine care in adult psychiatry. Parent‐rated questionnaire data were collected at baseline, after 6 and 12 months. We used growth curve models to investigate the effect of intervention on child mental health problems (SDQ‐P Total Difficulties) and perceived parental control of child behaviour (PLOC‐PPC).ResultsParents in the FTI and LTC groups, versus the IAU group, reported more favourable development in terms of preventing increase in child mental health problems with standardised intervention effects of d = −0.86 and −0.88 respectively, by study end, and reported improved perceived parental control, d = 1.08 and 0.71, respectively, by study end. No significant differences in effect were found when FTI and LTC were compared.ConclusionsThe results support continued use of FTI and LTC in adult psychiatry, and since LTC is a brief intervention, it might be useful as a minimum‐level preventive intervention.