bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanical communication-associated cell directional migration and branching connections mediated by calcium channels, integrin β1, and N-cadherin
Authors: Ouyang Mingxing , Zhu Yiming , Wang Jiajia , Zhang Qingyu , Hu Yanling , Bu Bing , Guo Jia , Deng Linhong 
Published: August 2022

Cell–cell mechanical communications at a large spatial scale (above hundreds of micrometers) have been increasingly recognized in recent decade, which shows importance in tissue-level assembly and morphodynamics. The involved mechanosensing mechanism and resulted physiological functions are still to be fully understood. Recent work showed that traction force sensation in the matrix induces cell communications for self-assembly. Here, based on the experimental model of cell directional migration on Matrigel hydrogel, containing 0.5 mg/ml type I collagen, we studied the mechano-responsive pathways for cell distant communications. Airway smooth muscle (ASM) cells assembled network structure on the hydrogel, whereas stayed isolated individually when cultured on glass without force transmission. Cell directional migration, or network assembly was significantly attenuated by inhibited actomyosin activity, or inhibition of inositol 1,4,5-trisphosphate receptor (IP3R) calcium channel or SERCA pump on endoplasmic reticulum (ER) membrane, or L-type calcium channel on the plasma membrane. Inhibition of integrin β1 with siRNA knockdown reduced cell directional migration and branching assembly, whereas inhibition of cell junctional N-cadherin with siRNA had little effect on distant attractions but blocked branching assembly. Our work demonstrated that the endoplasmic reticulum calcium channels and integrin are mechanosensing signals for cell mechanical communications regulated by actomyosin activity, while N-cadherin is responsible for traction force-induced cell stable connections in the assembly.
Effects of ultrafiltration on Co-Metal Organic Framework/pre-hydrolysis solution carbon materials for supercapacitor energy storage
Authors: Li Changwei , Sha Lei , Yang Kang , Kong Fangong , Li Peng , Tao Yubo , Zhao Xin , Chen Honglei 
Published: August 2022

Here, a Co-Metal Organic Framework/pre-hydrolysis (Co-MOF/pre-hydrolysis) solution carbon material is prepared by a mild and environmentally-friendly hydrothermal carbonization technique using a pulping pre-hydrolysis solution as the raw material and Co-MOF as the metal dopant. The stable hollow structure provide sufficient space for particle shrinkage and expansion, while the low density and large specific surface area of the long, hairy tentacle structure provide a greater contact area for ions, which shorten the transmission path of electrons and charges. The materials exhibit excellent specific capacitance (400 F/g, 0.5 A/g) and stability (90%, 10,000 cycles). The Change of different concentration ratios in the structures significantly affect the electrochemical performance. The specific surface area of the carbon materials prepared by ultra-filtration increased, but the specific surface area decrease as ultrafiltration concentration increase. The specific capacitance decrease from 336 F/g for C-ZIF-67-1/3 volume ultrafiltration to 258 F/g for C-ZIF-67-1/5 ultrafiltration. The results indicate that energy storage by the carbon materials relied on a synergistic effect between their microporous and mesoporous structures. The micropores provide storage space for the transmission of ions, while the mesopores provide ion transport channels. The separation of large and small molecules after ultrafiltration concentration limit the ion transmission and energy storage of the pores.
Association of serum pepsinogens and gastrin-17 with Helicobacter pylori infection assessed by urea breath test
Authors: Zhou Jun-peng , Liu Chang-hai , Liu Bo-wen , Wang You-juan , Benghezal Mohammed , Marshall Barry James , Tang Hong , Li Hong 
Published: August 2022

BackgroundAssociation of gastric atrophy or cancer with levels of serum pepsinogens, gastrin-17 and anti-Helicobacter pylori IgG antibody have been extensively studied. However, the association of serum pepsinogen and gastrin-17 with H. pylori infection has not been studied in a large population.AimTo investigate the impact of H. pylori infection on serum levels of pepsinogens and gastrin-17.MethodsA total of 354, 972 subjects who underwent health check-ups were included. Serum levels of pepsinogens and gastrin-17 were measured using the enzyme-linked immunosorbent assay. H. pylori infection was detected using 14C-urea breath test (UBT). Multivariable logistic regression analysis was used to investigate the association of serum pepsinogen and gastrin-17 with H. pylori infection.ResultsH. pylori prevalence was 33.18% in this study. The mean levels of pepsinogens and gastrin-17 were higher, while the mean pepsinogen-I/II ratio were lower among H. pylori-positive than -negative subjects. In H. pylori-positive subjects, pepsinogen and gastrin-17 levels correlated positively, whereas the pepsinogen-I/II ratio correlated negatively with UBT values (e.g., the mean serum level of pepsinogen-I in subjects with UBT values in the range of 100-499dpm, 500-1499dpm, and ≥1500dpm was 94.77 ± 38.99, 102.77 ± 43.59, and 111.53 ± 47.47 ng/mL, respectively). Compared with H. pylori-negative subjects, the adjusted odds ratio (aOR) of having pepsinogen-I ≤ 70 ng/mL in the three H. pylori-positive but with different UBT value groups was 0.31 (p&lt;0.001), 0.16 (p&lt;0.001), and 0.08 (p&lt;0.001), respectively; while the aOR of having G-17&gt;5.70 pmol/L was 4.56 (p&lt;0.001), 7.43 (p&lt;0.001), and 7.12 (p&lt;0.001). This suggested that H. pylori-positive subjects with higher UBT values were less likely to have pepsinogen-I ≤70 ng/mL (a serum marker for gastric atrophy), but more likely to have gastrin-17 &gt;5.70 pmol/L (a marker for peptic ulcer).ConclusionsH. pylori-positive subjects with higher UBT values are unlikely to have gastric atrophy, but may have greater risk of severe gastritis or peptic ulcers. Our study suggests that H. pylori-positive patients with high UBT values may benefit the most from H. pylori eradication.
Automatic rating of therapist facilitative interpersonal skills in text: A natural language processing application
Authors: Zech James M. , Steele Robert , Foley Victoria K. , Hull Thomas D. 
Published: August 2022

BackgroundWhile message-based therapy has been shown to be effective in treating a range of mood disorders, it is critical to ensure that providers are meeting a consistently high standard of care over this medium. One recently developed measure of messaging quality–The Facilitative Interpersonal Skills Task for Text (FIS-T)–provides estimates of therapists’ demonstrated ability to convey psychotherapy's common factors (e.g., hopefulness, warmth, persuasiveness) over text. However, the FIS-T's scoring procedure relies on trained human coders to manually code responses, thereby rendering the FIS-T an unscalable quality control tool for large messaging therapy platforms.ObjectiveIn the present study, researchers developed two algorithms to automatically score therapist performance on the FIS-T task.MethodsThe FIS-T was administered to 978 messaging therapists, whose responses were then manually scored by a trained team of raters. Two machine learning algorithms were then trained on task-taker messages and coder scores: a support vector regressor (SVR) and a transformer-based neural network (DistilBERT).ResultsThe DistilBERT model had superior performance on the prediction task while providing a distribution of ratings that was more closely aligned with those of human raters, versus SVR. Specifically, the DistilBERT model was able to explain 58.8% of the variance (R2 = 0.588) in human-derived ratings and realized a prediction mean absolute error of 0.134 on a 1–5 scale.ConclusionsAlgorithms can be effectively used to ensure that digital providers meet a consistently high standard of interactions in the course of messaging therapy. Natural language processing can be applied to develop new quality assurance systems in message-based digital psychotherapy.
Dynamic strain anomalies detection at Stromboli before 2019 vulcanian explosions using machine learning
Authors: Romano Pierdomenico , Di Lieto Bellina , Scarpetta Silvia , Apicella Ilenia , Linde Alan T. , Scarpa Roberto 
Published: August 2022

Identifying and characterizing the dynamics of explosive activity is impelling to build tools for hazard assessment at open-conduit volcanoes: machine learning techniques are now a feasible choice. During the summer of 2019, Stromboli experienced two paroxysmal eruptions that occurred in two different volcanic phases, which gave us the possibility to conceive and test an early-warning algorithm on a real use case: the paroxysm on July, 3 was clearly preceded by smaller and less perceptible changes in the volcano dynamics, while the second paroxysm, on August 28 concluded the eruptive phase. Among the changes observed in the weeks preceding the July paroxysm one of the most significant is represented by the shape variation of the ordinary minor explosions, filtered in the very long period (VLP 2–50 s) band, recorded by the Sacks-Evertson strainmeter installed near the village of Stromboli. Starting from these observations, the usage of two independent methods (an unsupervised machine learning strategy and a cross-correlation algorithm) to classify strain transients falling in the ultra long period (ULP 50–200 s) frequency band, allowed us to validate the robustness of the approach. This classification leads us to establish a link between VLP and ULP shape variation forms and volcanic activity, especially related to the unforeseen 3 July 2019 paroxysm. Previous warning times used to precede paroxysms at Stromboli are of a few minutes only. For paroxysmal events occurring outside any long-lasting eruption, the initial success of our approach, although applied only to the few available examples, could permit us to anticipate this time to several days by detecting medium-term strain anomalies: this could be crucial for risk mitigation by prohibiting access to the summit. Our innovative analysis of dynamic strain may be used to provide an early-warning system also on other open conduit active volcanoes.
Research on Power System Joint Optimal Generation Scheduling Based on Improved Balance Optimizer
Authors: Xu Jianming , Liu Anfeng , Qin Yang , Xu Guangrong , Tang Yibo 
Published: August 2022

This article presents a power system joint optimization generation regulation method based on the improved balance optimizer, which takes the five factors of power system network loss, voltage offset, generation cost, fuel cost, and comprehensive pollution emission as the objective function and takes the internal power balance of the system, each generator set, generation capacity, generation flow, and up and down climbing as the constraints. Fully considering the current energy-saving development objectives and the impact of economic dispatching, taking stable and safe operation as the core, the power generation dispatching model is established by improving the balance optimizer. The model realizes the maximum power generation with the lowest energy consumption parameters and transitions from the original power generation energy consumption of the power system to the best energy-saving power generation energy consumption so that the power value of the system reaches the target balance and completes efficient dispatching. Simulation experiments show that the proposed method can ensure the most reasonable power load in both summer and winter. The average load in summer and winter is reduced from 254.78/mw to 205.36/mw, down about 19.39%, which can ensure the power generation stability of the power system. The average power generation cost after dispatching is 129,920 $/h, which is significantly improved by comparing with 131,225 $/h before dispatching and can realize certain environmental benefits.
Long-Term Successive Biochar Amendments Alter the Composition and α-Diversity of Bacterial Community of Paddy Soil in Rice-Wheat Rotation
Authors: Xu Yunxiang , He Lili , Chen Jinyuan , Lyu Haohao , Wang Yuying , Yang Lie , Yang Shengmao , Liu Yuxue 
Published: August 2022

The specific effect of long-term successive biochar application on soil fertility, α-diversity, and composition of bacterial community and their correlations remain unclear. A field experiment was conducted to investigate the effects of straw biochar application on soil physical and chemical properties and the diversity and composition of the bacterial communities in 12 consecutive crop seasons. Four treatments: BC1 (2.25 Mgha−1 biochar), BC5 (11.25 Mgha−1 biochar), RS (2.25 Mgha−1 rice straw), and blank control (CK, without biochar or rice straw) were set up. The results indicated that biochar and rice straw reduced the richness indexes of the soil bacterial community (Chao1 and ACE by 10.3%–27.4% and 12.2%–26.4%, respectively). High-throughput sequencing results showed that the relative abundance of Cyanobacteria, Bacteroidetes, and Actinobacteria increased as the amount of biochar increased, while those of norank_c__Acidobacteria and norank_f__Anaerolineaceae Proteobacteria, Acidobacteria and Nitrospirae decreased. Long-term successive biochar application significantly increased soil pH, available potassium, total organic carbon, total nitrogen, and cation exchange capacity by 5.7%–25.9%, 40.0%–680.0%, 48.0%–217.1%, 51.0%–109.5%, and 5.4%–24.0%, respectively. Soil dissolved organic carbon was slightly decreased by 1.4%–4.0%. Soil pH and C/N were the two major environmental factors affecting the composition of the soil bacterial community according to redundancy analysis. Furthermore, the phylogenetic investigation of communities by reconstruction of unobserved states showed that biochar significantly increased the relative abundance of information on the functions of the metabolism of other amino acids, metabolism of terpenoids and polyketides, and biosynthesis of other secondary metabolites (p &lt; 0.05). Therefore, long-term successive biochar amendment in rice-wheat rotation systems improved soil fertility, altered the structure of the soil bacterial community, and increased the functions of soil bacteria, but decreased the α-diversity of the bacterial community. This study will provide technical and theoretical support for rice-straw carbonization and long-term soil remediation from the perspective of microorganisms.
Distribution and microbial degradation of dissolved organic carbon in the northern South China Sea
Authors: Ding Ling , Shan Sen , Luo Chunle , Wang Xuchen 
Published: August 2022

Dissolved organic carbon (DOC) is the largest reduced carbon pool in the ocean, and it plays significant roles not only in the ocean carbon cycle but also in the control of many biogeochemical processes in the ocean. We present the concentrations and distribution of DOC in the northern South China Sea (SCS) and western North Pacific (NP) in the spring and summer seasons of 2015-2016 and 2019. Laboratory incubation bioassay experiments were also conducted to determine the microbiological respiration of DOC. In the SCS, the concentrations of DOC varied within a range of 38-95 μM, and the large spatial variations in DOC in the upper 100 m depth were influenced by a combination of factors, including primary production, terrestrial inputs from the Pearl River and the intrusion of the Kuroshio Current. The mesopelagic DOC distribution in the northern SCS basin was largely influenced by the physical mixing of upwelled deep water; however, biological processes were estimated to account for 6-20% of the modulation in DOC concentrations. Compared with the deep DOC levels in open ocean areas, a slightly excessive DOC concentration (~ 3-4 μM) was observed in the deep water of the SCS basin. Approximately 10-20% of the DOC was consumed by mesopelagic and/or deep water bacteria in the incubation bioassay experiments, and labile DOC was preferentially respired, resulting in decreased δ13C and Δ14C values of DOC.
The Clinical Frailty Scale (CFS) employment in the frailty assessment of patients suffering from Non-Communicable Diseases (NCDs): A systematic review
Authors: Granata Nicolò , Vigoré Martina , Steccanella Andrea , Ranucci Luca , Sarzi Braga Simona , Baiardi Paola , Pierobon Antonia 
Published: August 2022

BackgroundThe Clinical Frailty Scale (CFS) is a well-established tool that has been widely employed to assess patients' frailty status and to predict clinical outcomes in the acute phase of a disease, but more information is needed to define the implications that this tool have when dealing with Non-Communicable Diseases (NCDs).MethodsAn electronic literature search was performed on PubMed, Scopus, EMBASE, Web of Science, and EBSCO databases to identify studies employing the CFS to assess frailty in patients with NCDs.FindingsAfter database searching, article suitability evaluation, and studies' quality assessment, 43 studies were included in the systematic review. Researches were conducted mostly in Japan (37.5%), and half of the studies were focused on cardiovascular diseases (46.42%), followed by cancer (25.00%), and diabetes (10.71%). Simplicity (39.29%), efficacy (37.5%), and rapidity (16.07%) were the CFS characteristics mostly appreciated by the authors of the studies. The CFS-related results indicated that its scores were associated with patients' clinical outcomes (33.92%), with the presence of the disease (12.5%) and, with clinical decision making (10.71%). Furthermore, CFS resulted as a predictor of life expectancy in 23 studies (41.07%), clinical outcomes in 12 studies (21.43%), and hospital admissions/readmissions in 6 studies (10.71%).DiscussionCFS was found to be a well-established and useful tool to assess frailty in NCDs, too. It resulted to be related to the most important disease-related clinical characteristics and, thus, it should be always considered as an important step in the multidisciplinary evaluation of frail and chronic patients.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.asp? PROSPERO 2021, ID: CRD42021224214.
Benefits of a 3-month cycle of weekly virtual museum tours in community dwelling older adults: Results of a randomized controlled trial
Authors: Beauchet Olivier , Matskiv Jacqueline , Galery Kevin , Goossens Linda , Lafontaine Constance , Sawchuk Kim 
Published: August 2022

BackgroundMuseums can be instrumental in fostering social inclusion and may improve the overall health of the older population. Over the course of the 2019 coronavirus pandemic, many older adults suffered as a result of confinement measures, which may have accelerated the processes that lead to physical frailty and increased mental health risks. This study aims to examine whether a 3-month cycle of weekly virtual tours of the Montreal Museum of Fine Arts (MMFA) may have improved feelings of social inclusion, wellbeing and quality of life, and reduced physical frailty in older adults living within the community of Montreal.Methods and designA total of 106 older adults, who were community-dwellers living in Montreal (Quebec, Canada), were recruited for a randomized controlled trial in two parallel groups (intervention with n = 53 vs control with n = 53) between January and April 2022. The intervention consisted of a 3-month cycle of weekly virtual museum tours of the MMFA. Social isolation, wellbeing, quality of life and frailty were evaluated using validated scales that were assessed on a web platform at baseline (M0) and after 3 months (M3) in the intervention group. The control group completed the same assessment according to the same schedule. The outcomes were the mean scores at M0 and M3, and changes in mean scores between M0 and M3.ResultsThe intervention group showed significant improvements in their social isolation, wellbeing, quality of life and frailty scores when compared to the control group, the highest benefits being observed with frailty.ConclusionThe results suggest that the 3-month cycle of weekly virtual MMFA tours may improve social inclusion, physical and mental health in community-dwelling older adults living in Montreal.Trial registrationhttps://clinicaltrials.gov/ct2/show/NCT05046288, identifier NCT05046288.
A comprehensive prognostic and immunological analysis of ephrin family genes in hepatocellular carcinoma
Authors: Huang Shenglan , Dong Cairong , Zhang Jian , Fu Shumin , Lv Yaqin , Wu Jianbing 
Published: August 2022

Background: Ephrins, a series of Eph-associated receptor tyrosine kinase ligands, play an important role in the tumorigenesis and progression of various cancers. However, their contributions to hepatocellular carcinoma (HCC) remain unclear. Thus, we aimed to explore their prognostic value and immune implications in HCC.Methods: Multiple public databases, such as TCGA, GTEx, and UCSC XENA, were used to analyze the expression of ephrin genes across cancers. Kaplan-Meier analysis and Cox regression were used to explore the prognostic role of ephrin genes in HCC. A logistic regression model was utilized to evaluate the association between ephrin gene expression and clinical characteristics. Gene set enrichment analysis (GSEA) was conducted to elucidate their potential biological mechanisms. Various immune algorithms were utilized to investigate the correlation between ephrin genes and tumor immunity. We also analyzed their association with drug sensitivity, and gene mutations. Finally, RT–qPCR was performed to validate the expression of ephrin family genes in HCC cells and clinical tissues.Results: The expression of EFNA1, EFNA2, EFNA3, EFNA4, EFNB1, and EFNB2 was upregulated in most cancer types, while EFNA5 and EFNB3 was downregulated in most cancers. In HCC, the expression levels of EFNA1, EFNA3, EFNA4, EFNB1, and EFNB2 were significantly higher in tumor tissues than in normal tissues. High expression of EFNA3, EFNA4, and EFNB1 was associated with tumor progression and worse prognosis in HCC patients. The expression of EFNA3 and EFNA4 was negatively associated with the stromal/ESTIMATE scores, while EFNB1 was positively correlated with the immune/stromal/ESTIMATE scores. Moreover, these ephrin genes were closely relevant to the infiltration of immune cells, such as B cells, CD4+ T cells, CD8+ T cells, neutrophil cells, macrophage cells, and dendritic cells. EFNB1 expression was positively associated with most immune-related genes, while EFNA3/EFNA4 was positively related to TMB and MSI. In addition, EFNA3, EFNA4, and EFNB1 were related to drug sensitivity and affected the mutation frequency of some genes in HCC.Conclusion: EFNA3, EFNA4, and EFNB1 are independent prognostic factors for HCC patients and are closely correlated with tumor immunity, which may provide a new direction for exploring novel therapeutic targets and biomarkers for immunotherapy.