bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-Term Follow-up in Labiaplasty in 414 Women
Authors: Qiang Shuai , Li Feng Yong , Zhou Yu , Cao Yu Jiao , Liu Mei Chen , Li Qiang 
Published: September 2022

Abstract
Women with labial hypertrophy may opt for labiaplasty. The aim of the study was to analyze the complications, long-term follow-up of clinical outcomes and satisfaction in this population over the past 10 years.
All women who underwent labiaplasty with or without clitoral hood reduction at our center between January 2008 and August 2020 were retrospectively identified. Retrospective study was conducted, recording follow-up outcomes, motivations, techniques, postoperative complications, reasons requiring reoperation, and surgical satisfaction concerned different clinical characteristics. A concrete analysis of each specific outcome and a detailed knowledge of these procedures were performed.
A total of 52.41% reported functional impairment, 20.25% cited aesthetics complains, and 27.34% were motivated by both the previously mentioned reasons. Women undergoing surgical correction sometimes mixed with various psychological aspects. A total of 91.06% reported that they were moderately (6.04%) or extremely (85.02%) satisfied with the outcomes after surgery, and 37 (8.94%) reported dissatisfied. Revision surgery was needed in a small number of patients (n = 7 of 414 patients, 1.69%) because of undesirable appearance. The most common complication in our patients was asymmetry (6.28%). Women requested labiaplasty in the last 2 reasons or procedures or without psychological distress were more likely feel satisfied with the results (satisfaction rate >90%). There was a significant difference in satisfaction among the 3 concerns as well as between the 2 kinds of psychological conditions (P < 0.05). Satisfaction of patients with aesthetic complains compared with the other 2 worries was significantly lower (P < 0.05). There was no significant difference among the 3 procedures in this comparison group (P > 0.05). In addition, satisfaction in labiaplasty with or without clitoral hoodoplasty, neither of which was statistically different (P > 0.05). Patients aged 30 to 39 years reported the lowest satisfaction in comparison with the other age groups.
Labiaplasty of the labia minora is the most commonly performed genital cosmetic surgery procedure. This study on 414 patients demonstrated that it is associated with a high degree of patient satisfaction and low morbidity and provided evidence of safety and effectiveness preliminary, indicating that the procedure may be regarded as safe.
Comprehensive Management of Infantile Hemangiomas Involving the Periorbital Region
Authors: Chen Wei-liang , Lan Yu-qing , Hong Lei , Dong Xiao-yu 
Published: September 2022

Abstract
Periocular infantile hemangioma (pIH) is associated with a risk of vision loss and requires urgent medical intervention. We evaluated the outcomes of the comprehensive management of 44 pIH patients (aged 2–6 months) with superficial (n = 11), deep (n = 15), and mixed (n = 18) subtypes, treated with a standard propranolol dose (2 mg kg−1 d−1) without any obvious side effects. Obvious and slight improvements were observed in 70.5% and 29.5% of patients, respectively. Obvious improvement was found in 96.3% of patients 3 months or younger but only 29.4% of patients older than 3 months (P = 0.036) after 4 weeks of treatment. Thirteen patients demonstrated slight improvement and were treated with an increased propranolol dose (3 mg kg−1 d−1) in combination with corticosteroid injections or plastic surgery, which led to good outcomes. The patients were followed up for 12 to 18 months, with no cases of amblyopia or ametropia. Oral propranolol was effective in the treatment of pIH. Earlier propranolol use was associated with better outcomes. An increased propranolol dose combined with corticosteroid injections or plastic surgery is appropriate for patients with propranolol resistance.
Increased lipid peroxidation and lowered lipid-associated antioxidant defenses mediate the effects of the paraoxonase 1 (PON1) Q192R polymorphism on disabilities and final stroke core volume in mild and moderate stroke
Authors: Maes Michael , Brinholi Francis F. , Michelin Ana Paula , Matsumoto Andressa K. , Semeão Laura de O. , Almulla Abbas F. , Supasitthumrong Thitiporn , Tunvirachaisakul Chavit , Barbosa Decio S. 
Published: September 2022

AbstractIn acute ischemic stroke (AIS), there are no data on whether lipid and protein oxidation and antioxidant biomarkers are associated with the outcome of AIS above and beyond the effects of traditional risk factors, immune and metabolic biomarkers, and measurements of stroke volume. The present study was conducted in 135 mild to moderate AIS patients and 40 controls and assessed the modified raking scale (mRS) at baseline, and 3 and 6 month later. We measured lipid hydroperoxides (LOOH), malondialdehyde (MDA), advanced oxidation protein products, paraoxonase 1 (PON1) activities and PON1 Q192R genotypes, high density lipoprotein cholesterol (HDL), sulfhydryl (-SH) groups), and diffusion-weighted imaging (DWI) stroke volume and fluid-attenuated inversion recovery (FLAIR) signal intensity. We found that a) AIS is characterized by lower chloromethyl acetate (CMPA)ase PON1 activity, HDL and -SH groups and increased LOOH and neurotoxicity (a composite of LOOH, inflammatory markers and glycated hemoglobin); b) oxidative and antioxidant biomarkers strongly and independently predict mRS scores 3 and 6 months later and DWI stroke volume and FLAIR signal intensity; c) the PON1 Q192R variant has multiple effects on stroke outcome that are mediated by its effects on antioxidant defenses and lipid peroxidation; and d) the PON1 RR variant has a protective effect against lipid peroxidation, neurotoxicity, infarct volume and stroke outcome. Increased lipid peroxidation and lowered activity of the PON1-HDL complex and -SH groups are drug targets to prevent AIS and consequent neurodegenerative processes with loss of function, and increased levels of oxidative reperfusion mediators due to ischemia-reperfusion injury.
The Lactate Gradient, an unrecognized biomarker of potential significance
Authors: Fabricius Thomas 
Published: September 2022

AbstractBackgroundHuman lactate formation is typically attributed to hypoxia, but it may actually be due to the influence of norepinephrine.MethodsA lactate gradient was measured from the first few drops of blood obtained after application of a lancet to a finger pad. However, the blood needed to be carefully obtained according to this protocol.ResultsThe measured lactate gradient was surprisingly large in some circumstances, and was not correlated with any of the other measured parameters such as oxygen or carbon dioxide blood levels. There were hints in the data that the lactate gradient seemed to be influenced by sympathetic noradrenergic nervous system activity.ConclusionsThe lactate gradient is relatively easily obtainable, and may reflect conditions causing enhanced sympathetic activity.
In situ single-cell analysis of canonical breast cancer biomarkers: phenotypic heterogeneity and implications on response to HER2 targeting agents
Authors: Serna Garazi , García Eloy , Fasani Roberta , Guardia Xavier , Pascual Tomas , Paré Laia , Ruiz-Pace Fiorella , Llombart-Cussac Antonio , Cortes Javier , Prat Aleix , Nuciforo Paolo 
Published: September 2022

ABSTRACTBreast cancer is a heterogeneous disease. Tumor cells and the surrounding microenvironment form an ecosystem that determine disease progression and response to therapy. To characterize the breast cancer ecosystem and the changes induced by targeted treatment selective pressure, we analyzed 136 HER2-positive tumor samples for the expression of canonical BC tumor diagnostic proteins at a single cell level without disrupting the spatial context. The combined expression of HER2, ER, PR, and Ki67 in more than a million cells was evaluated using a tumor-centric panel combining the four biomarkers in a single tissue section by sequential immunohistochemistry to derive 16 tumor cell phenotypes. Spatial interactions between individual tumor cells and cytotoxic T cells were studied to determine the immune characteristics of the ecosystem and the impact on response to treatment. HER2-positive tumors displayed individuality in tumor cells and immune cells composition, including intrinsic phenotype dominance which only partially overlapped with molecular intrinsic subtyping determined by PAM50 analysis. This single cell analysis of canonical BC biomarkers deepens our understanding of the complex biology of HER2-positive BC and suggests that individual cell-based patient classification may facilitate identification of optimal responders or resistant individual to HER2-targeted therapies.
FishSCT: a zebrafish-centric database for exploration and visualization of fish single-cell transcriptome
Authors: Guo Cheng , Ye Weidong , Duan You , Zhang Wanting , Cheng Yingyin , Shi Mijuan , Xia Xiao-Qin 
Published: September 2022

AbstractWith the advancement of single-cell sequencing technology in recent years, an increasing number of researchers have turned their attention to the study of cell heterogeneity. In this study, we created a fish single-cell transcriptome database centered on zebrafish (Danio rerio). FishSCT currently contains single-cell transcriptomic data on zebrafish and 8 other fish species. We used a unified pipeline to analyze 129 datasets from 44 projects from SRA and GEO, resulting in 964/26,965 marker/potential marker information for 245 cell types, as well as expression profiles at single-cell resolution. There are 117 zebrafish datasets in total, covering 25 different types of tissues/organs at 36 different time points during the growth and development stages. This is currently the largest and most comprehensive online resource for zebrafish single-cell transcriptome data, as well as the only database dedicated to the collection of marker gene information of specific cell type and expression profiles at single-cell resolution for a variety of fish. A user-friendly web interface for information browsing, cell type identification, and expression profile visualization has been developed to meet the basic demand in related studies on fish transcriptome at the single-cell resolution.
Wound healing and anti-inflammatory potential of Ajuga bracteosa-conjugated silver nanoparticles in Balb/c mice
Authors: Andleeb Saiqa , Nazer Sadia , Alomar Suliman Yousef , Ahmad Naushad , Khan Imran , Raza Abida , Awan Uzma Azeem , Raja Sadaf Azad 
Published: September 2022

AbstractBackgroundWound therapy is complicated, uncomfortable for the patient, and costly for the health-care system. Silver nanoparticles (AgNP) have antibacterial characteristics that can prevent bacterial infection in wounds and speed up wound healingObjectiveThe aim of current research was to investigate the wound healing and anti-inflammatory potential of biogenic synthesized silver nanoparticles (ABAgNP) using Ajuga bracteosa (ABaqu) in Swiss albino mice.MethodsIn vivo wound healing and anti-inflammatory activities were carried out using Bala/c mice. For in vivo screening of 200 mg/kg and 400 mg/kg of both ABAgNPs and ABaqu were used. Liver and kidney functional markers, hematology, and histopathological studies were carried out after 14 days of administration.ResultsThe obtained biogenic nanoparticles were characterized, dermal toxicity, wound excision repairing, and formalin-induced paw edema assays were performed in Swiss albino mice. Dermal toxicity showed that tested concentrations of ABaqu and ABAgNPs were safe. No adverse effects, changes, and alteration in the skin of treatment groups as well as the control vehicle group (petroleum jelly) were recorded. Results revealed that the enhanced wound contraction was observed in ABaqu, ABAgNP, and the Nitrofuranose treated groups from 7th to 11thdays. The anti-inflammatory activity in formalin-induced paw edema model illustrated the potential use of silver nanoparticles ABAgNPs and ABaqu as a reducing or inflammation inhibiting agents due to the release of acute inflammatory mediators.ConclusionTherefore, it was concluded that both silver nanoparticles (ABAgNP) and Ajuga bracteosa (ABaqu) extracts could be used as a wound healing and anti-inflammatory agents.
Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles
Authors: Kim Hyun Ji , Rames Matthew J. , Roskams-Hieter Breeshey , Briand Josephine , Doe Aaron , Estabrook Joseph , Wagner Josiah T. , Demir Emek , Mills Gordon , Ngo Thuy T. M. 
Published: September 2022

AbstractExtracellular vesicles (EVs) have been shown as key mediators of extracellular small RNA transport. However, carriers of cell-free messenger RNA (cf-mRNA) in human biofluid and their association with cancer remain poorly understood. Here, we performed a transcriptomic analysis of size-fractionated plasma from lung cancer, liver cancer, multiple myeloma, and healthy donors. Morphology and size distribution analysis showed the successful separation of medium and small EVs and non-vesicular carriers. We developed a strategy to purify and sequence ultra-low amounts of cf-mRNA from vesicular and non-vesicular subpopulations with the implementation of RNA spike-ins to control for technical variability and to normalize for intrinsic drastic differences in the amount of cf-mRNA carried in each plasma fraction. We found that the majority of cf-mRNA was enriched and protected in EVs with remarkable stability in RNase-rich environments. We observed specific enrichment patterns of cancer-associated cf-mRNA in each vesicular and non-vesicular subpopulation. The EV-enriched differentiating genes were associated with specific biological pathways, such as immune systems, liver function, and toxic substance regulation in lung cancer, liver cancer, and multiple myeloma, respectively. Our results suggest that dissecting the complexity of EVs subpopulations illuminates their biological significance and offers a promising liquid biopsy approach.
Science, policy and resilience: reflections on the role of scientific advice to government during pandemic crisis response
Authors: Reddin Karen , Miles Lee 
Published: September 2022

PurposeThe SARS epidemic in 2003 and the COVID-19 pandemic had a disruptive impact on countries around the world and highlight the importance of using scientific evidence to inform policy decisions and priorities during crises. The purpose of this article is to reflect upon the term “following the science” and examines the differences between SARS in 2003 and COVID-19.Design/methodology/approachThis study is exploratory, adopts a qualitative approach and reflects on the synthesis of scientific evidence into advice informing government decisions on health interventions. Random sampling of the literature was used to avoid bias and was guided by the keywords.FindingsIt considers preparedness activities and the need for these to be integral in the design of future planning. It argues that simulation exercises be intrinsically linked to all aspects of crisis management and provide the opportunity to use the scientific evidence base as part of preparedness planning. The article concludes that more transparency in the use of scientific advice in strategic decision-making would support building more resilience into health emergency preparedness through an integrated systems approach.Originality/valueThis article contributes to the literature on the evaluation of the “following the science” approach and its implementation. It also contributes to the limited literature on simulation exercising to deal with health crises, like pandemics and identifies potential areas for further research or work on developing an integrated systems approach to pandemic preparedness.
Investigating the factors affecting construction site productivity – a case of India
Authors: Vigneshwar R.V.K. , Shanmugapriya S. 
Published: September 2022

PurposeProper prediction of productivity can enable the enhanced estimation, realistic scheduling, and accurate cost forecasting of construction processes. Due to the existence of different labor sources (unionized and non-unionized), the prediction of productivity is still a significant problem in India. Moreover, the construction procurement processes and on-site performance are the predominant elements that can result in improved project outcomes. Thereby, the consideration of labor constraints and site conditions will play an important role in productivity improvement.Design/methodology/approachThis study investigates the factors affecting construction site productivity. A total of 28 factors are grouped under 7 categories as follows: labor constraints, safety and quality procurements, material and equipment (ME), site management, project working condition, delay controls, construction methods and techniques, and external factors. Furthermore, by involving these factors, the questionnaire survey was conducted among Indian construction practitioners. As a result, 204 responses were received and the data were analyzed using a reliability test, relative importance index (RII), and analysis of variance (ANOVA).FindingsThe result of this study highlighted the importance of strategic construction management activities in terms of effective planning of ME, planning and realistic scheduling of construction activities, proper communication, information sharing, etc. Thus, this study provides a clear insight to the Indian construction practitioners in determining the effect of these site factors on the successful execution of their projects.Originality/valueIn this paper, the problem of construction productivity in India and its causes are explained effectively. This study examines the preference of labor contract, labor source, and most importantly, the factors affecting site productivity. Moreover, the other lagging issues regarding the management of construction activities are also described in detail.
Theory of planned behavior and GEM framework – How can cognitive traits for entrepreneurship be used by incubators and accelerators?
Authors: Morales-Alonso Gustavo , Blanco-Serrano José Antonio , Núñez Guerrero Yilsy , Grijalvo Mercedes , Blanco Jimenez Francisco José 
Published: September 2022

PurposeThis research aims at the theory of planned behavior (TPB) and the global entrepreneurship monitor (GEM) framework – How can cognitive traits for entrepreneurship be used by incubators and accelerators?Design/methodology/approachIn this research the authors analyze the factors that catalyze the founding of new technology-based firms. From a practitioner stand-point, the GEM posits that these factors can be classified as contextual, social and individual factors. The present study focuses on the latter, looking into how demographic characteristics, possession of human capital and cognitive traits interrelate. The authors rely on a sample of 141 technological new ventures being incubated in Madrid, Spain, which is analyzed with the multilayer perceptron technique.FindingsThe results show that cognitive traits, as defined in the TPB, act as the “last mile” in the entrepreneurial decision process, while demographic and human capital factors appear to antecede them. These results are relevant for incubators and accelerators, which now gain a better, more complete understanding of success factors of their incubatees.Originality/valueThis research deals both with practitioners' view of entrepreneurship and with scientific literature, intertwining both with the purpose of providing valuable information for incubators and accelerators.
Development of critical infrastructure resilience index for cities in Sri Lanka
Authors: Perera Udara Sachinthana , Siriwardana Chandana , Pitigala Liyana Arachchi Ishani Shehara 
Published: September 2022

Purpose
Infrastructures become critical with the emerging threats triggering through disasters. Sri Lanka is a country with a higher risk of disaster impacts, in which the eye-opening has widened towards mitigating the damages towards critical infrastructures. Based on this, the purpose of this paper is to develop an index that identifies the significance of critical infrastructure resilience.

Design/methodology/approach
From the initial literature survey, disaster resilience is defined as capacity of three stages, absorptive, adaptive and restorative along with ten indicators to measure capacities. Selected indicators were then checked for suitability for scope of the research based on opinions of seven experts. Subsequently, the critical infrastructure resilience index (CIRI) was introduced such that the numerical values for each indicator are aggregated using the Z score method. Statistical relations between the actual impact against disasters and CIRI calculated for administrative regions in Sri Lanka were used as the final step to validate the developed index.

Findings
Resilience index development is presented in this paper with a comprehensive methodology of developing and validation. Further, the case study results imply the weakness and strengths in each resilience capacities, which are important in decision-making.

Research limitations/implications
Unavailability of disaster impact data and centralized data repository were main constrains in the validation process of this research. Hence proxy data was used to validate resilience index in this research.

Originality/value
This research identified and validated a novel approach of defining disaster resilience index for regional decision-making.
Switchover to industrial additive manufacturing: dynamic decision-making for problematic spare parts
Authors: Akmal Jan Sher , Salmi Mika , Björkstrand Roy , Partanen Jouni , Holmström Jan 
Published: September 2022

PurposeIntroducing additive manufacturing (AM) in a multinational corporation with a global spare parts operation requires tools for a dynamic supplier selection, considering both cost and delivery performance. In the switchover to AM from conventional manufacturing, the objective of this study is to find situations and ways to improve the spare parts service to end customers.Design/methodology/approachIn this explorative study, the authors develop a procedure – in collaboration with the spare parts operations managers of a case company – for dynamic operational decision-making for the selection of spare parts supply from multiple suppliers. The authors' design proposition is based on a field experiment for the procurement and delivery of 36 problematic spare parts.FindingsThe practice intervention verified the intended outcomes of increased cost and delivery performance, yielding improved customer service through a switchover to AM according to situational context. The successful operational integration of dynamic additive and static conventional supply was triggered by the generative mechanisms of highly interactive model-based supplier relationships and insignificant transaction costs.Originality/valueThe dynamic decision-making proposal extends the product-specific make-to-order practice to the general-purpose build-to-model that selects the mode of supply and supplier for individual spare parts at an operational level through model-based interactions with AM suppliers. The successful outcome of the experiment prompted the case company to begin the introduction of AM into the company's spare parts supply chain.