bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polycystin‐1 regulates cell proliferation and migration through AKT/mTORC2 pathway in a human craniosynostosis cell model
Authors: Katsianou Maria A. , Papavassiliou Kostas A. , Gargalionis Antonios N. , Agrogiannis George , Korkolopoulou Penelope , Panagopoulos Dimitrios , Themistocleous Marios S. , Piperi Christina , Basdra Efthimia K. , Papavassiliou Athanasios G. 
Published: March 2022

AbstractCraniosynostosis is the premature fusion of skull sutures and has a severe pathological impact on childrens’ life. Mechanical forces are capable of triggering biological responses in bone cells and regulate osteoblastogenesis in cranial sutures, leading to premature closure. The mechanosensitive proteins polycystin‐1 (PC1) and polycystin‐2 (PC2) have been documented to play an important role in craniofacial proliferation and development. Herein, we investigated the contribution of PC1 to the pathogenesis of non‐syndromic craniosynostosis and the associated molecular mechanisms. Protein expression of PC1 and PC2 was detected in bone fragments derived from craniosynostosis patients via immunohistochemistry. To explore the modulatory role of PC1 in primary cranial suture cells, we further abrogated the function of PC1 extracellular mechanosensing domain using a specific anti‐PC1 IgPKD1 antibody. Effect of IgPKD1 treatment was evaluated with cell proliferation and migration assays. Activation of PI3K/AKT/mTOR pathway components was further detected via Western blot in primary cranial suture cells following IgPKD1 treatment. PC1 and PC2 are expressed in human tissues of craniosynostosis. PC1 functional inhibition resulted in elevated proliferation and migration of primary cranial suture cells. PC1 inhibition also induced activation of AKT, exhibiting elevated phospho (p)‐AKT (Ser473) levels, but not 4EBP1 or p70S6K activation. Our findings indicate that PC1 may act as a mechanosensing molecule in cranial sutures by modulating osteoblastic cell proliferation and migration through the PC1/AKT/mTORC2 cascade with a potential impact on the development of non‐syndromic craniosynostosis.
Sleeping Sound Autism Spectrum Disorder (ASD): a randomised controlled trial of a brief behavioural sleep intervention in primary school‐aged autistic children
Authors: Papadopoulos Nicole , Sciberras Emma , Hiscock Harriet , Williams Katrina , McGillivray Jane , Mihalopoulos Cathrine , Engel Lidia , Fuller‐Tyszkiewicz Matthew , Bellows Susannah T. , Marks Deborah , Howlin Patricia , Rinehart Nicole 
Published: March 2022

BackgroundBehavioural sleep problems are common in children with autism spectrum disorder (ASD); however, evidence for the efficacy of behavioural sleep interventions is limited. This study examined the efficacy of a brief behavioural sleep intervention in autistic children. It was hypothesised that the intervention would reduce overall child sleep problems (primary outcome), in addition to improvements in children’s social, emotional, cognitive, academic functioning, and quality of life, and parent/caregivers’ stress, quality of life, and mental health (secondary outcomes).MethodsA randomised controlled trial was conducted with participants randomised via a computer‐generated sequence to the sleeping sound intervention (n = 123) or treatment as usual (n = 122) group. Participants comprised 245 children with an ASD diagnosis. Inclusion criteria were as follows: confirmation of DSM IV or DSM‐5 diagnosis of ASD, participants aged between 5 and 13 years and parent/caregiver report of moderate–severe sleep problems. Exclusion criteria were as follows: parent/caregiver intellectual disability or lacking sufficient English to complete questionnaires; and child participant with co‐occurring medical conditions known to impact sleep. The intervention group received the sleeping sound intervention (2 × 50‐min face‐to‐face sessions plus follow‐up phone call) by a trained clinician.ResultsChange in children’s sleep problems was measured by the Children’s Sleep Habits Questionnaire (CSHQ) at 3 months post randomisation. Parents/caregivers of children in the intervention group reported a reduction in child sleep problems at 3 months post randomisation (effect size: E.S −0.7). There were also small effects in a number of child (internalising symptoms, emotional behavioural disturbance and quality of life) and parent/caregiver (mental health, parenting stress and quality of life) outcomes; however, these did not remain significant when controlling for multiple comparisons.ConclusionsThe sleeping sound ASD intervention is an efficacious and practical way to reduce sleep problems for autistic children. This brief behavioural intervention has the potential to be embedded easily into the Australian healthcare system.
Minocycline suppresses lipogenesis via inhibition of p300 histone acetyltransferase activity in human SZ95 sebocytes
Authors: Shin H.S. , Zouboulis C.C. , Kim M.K. , Lee D.H. , Chung J.H. 
Published: March 2022

AbstractBackgroundMinocycline is a second‐generation tetracycline drug, which is widely used to treat diverse infectious and inflammatory diseases such as acne vulgaris. The effects of minocycline on acne vulgaris have been mainly attributed to its anti‐inflammatory effect; however, its sebum‐regulating effect and the relevance to epigenetic regulation in human sebaceous glands remain uninvestigated.ObjectivesTo identify the potential underlying epigenetic mechanism of sebum‐inhibitory effects of minocycline in human SZ95 sebocytes.MethodsThe quantity of lipid droplets and the expression of key lipogenic genes were analysed in minocycline‐treated SZ95 sebocytes. To examine whether the sebum‐inhibitory effects of minocycline are relevant to histone acetylation, we analysed the effects of minocycline on p300 HAT and total HDAC activity. To elucidate the functional implication of p300 HAT inhibition by minocycline in sebocytes, we assessed the effect of p300 knockdown, inhibition and overexpression on lipid accumulation in SZ95 sebocytes.ResultsMinocycline suppressed the insulin and liver X receptor agonist‐induced lipid accumulation and the expression of the key lipogenic transcription factor sterol regulatory element‐binding protein 1 (SREBP1) and its downstream genes, fatty acid synthase (FAS) and acetyl‐CoA carboxylase α (ACCα). Minocycline inhibited p300 HAT activity in a concentration‐dependent manner, but demonstrated no effect on global HDAC activity, resulting in a significant decrease in histone acetylation. Downregulation of p300 by knockdown or inhibition significantly suppressed SREBP1 expression, histone acetylation and lipid accumulation, whereas p300 overexpression enhanced these effects. Moreover, p300 overexpression rescued minocycline‐inhibited SREBP1 expression and lipid synthesis.ConclusionsOur findings revealed a novel sebum‐regulating effect of minocycline. Moreover, as p300 HAT is a key epigenetic regulator of sebaceous lipogenesis, its inhibitors could be used for the treatment of acne vulgaris.
Acceptance of COVID‐19 vaccines in patients with chronic diseases: A cross‐sectional study
Authors: Jiang Ning , Gu Pengfei , Sun Xian , Han Hui , Liu Wenwen , Song Na , Jiang Xiaolian 
Published: March 2022

AbstractAims and objectivesThis study aimed to clarify the attitudes, knowledge and vaccination willingness of patients with chronic diseases toward COVID‐19 vaccines and the influencing factors.BackgroundVaccination against COVID‐19 is an important way to protect patients with chronic diseases, but the vaccination acceptance varies across diseases and populations. A better understanding of this condition will lead to tailored intervention strategies and high vaccination rates.DesignCross‐sectional study.MethodsData were collected between March 2021 and May 2021 in China. A self‐compiled questionnaire was used in the survey. Two independent‐samples t‐tests/one‐way analysis of variance or U test/H test was used to measure the differences between groups. Multivariate regression analysis was used to identify the influencing factors. The study adhered to the EQUATOR checklist, STROBE.ResultsA total of 998 patients participated in the study. Score rates of attitudes, knowledge and vaccination willingness were 69.9%, 68.4% and 70.6% respectively. Age, vaccination status of family members, education levels, vaccine side effects and economic level were positive factors that could influence patients’ vaccination acceptances, while time of illness, type of disease and political affiliations were negative predictors. The top reasons for willingness toward vaccination were supporting national strategies, belief on the vaccines and fearing of contracting COVID‐19, while physical reasons, side effects and having a wait‐and‐see attitude were unwillingness factors.ConclusionsPatients’ attitudes, knowledge and vaccination willingness were medium. Nurses should pay attention to patients who are from lower socioeconomic backgrounds, under 30 or over 70 years old, have no political affiliations, have damage to vital organs, have a long course of illness, family members have not received COVID‐19 vaccines and had no side effects after receiving other vaccines.Relevance to clinical practiceClinical nurses are recommended to take measures from patients’ duration of illness, damaged organs, demographic characteristics and families to improve patients’ vaccination acceptances.
Japanese Clinical Psychologists' Consensus Beliefs about Mental Health: A Mixed‐Methods Approach
Authors: Sunohara Momoka , Sasaki Jun , Kogo Sonora , Ryder Andrew G. 
Published: March 2022

AbstractThis study applied a two‐phase, mixed‐methods research design, grounded in cultural consensus theory (CCT), to examine shared beliefs about mental health held by Japanese clinical psychologists (CPs). In CCT, qualitative methods are first used to identify culturally salient elements of a domain; factor analysis is then used to quantify the degree of sharedness, an approach known as cultural consensus analysis (CCA). First, a free‐listing technique with 16 Japanese CPs was conducted to elicit salient terms for the two domains: (a) how members of the general public acquire beliefs about mental health; and (b) how Japanese mental healthcare ought to be reformed. In the second phase, CCA was conducted through a survey completed by 100 CPs. The free‐listing analysis generated 21 and 23 culturally salient terms for the two domains, respectively. Then, CCA demonstrated that the two domains could each be characterized as a single cultural model with a high degree of consensus. CCT provides a systematic mixed‐methods approach that is particularly well‐suited to investigating culturally grounded shared beliefs held by people in a specific cultural context.
Biopriming of maize seeds with plant growth-promoting bacteria isolated from the earthworm Aporrectodea molleri: effect on seed germination and seedling growth
Authors: Houida S. , Yakkou L. , Kaya L.O. , Bilen S. , Fadil M. , Raouane M. , El Harti A. , Amghar S. 
Published: March 2022

Abstract
Earthworms have become a potential source of multi-beneficial bacteria and effective bioinoculants. Seed biopriming is an efficient inoculation method to apply bacteria prior to sowing, which enhances the chances of bacterial candidates to colonize the rhizosphere and/or establish a liaison with the plant. In this study, we evaluated plant growth-promoting traits of bacterial strains isolated from the earthworm’s Aporrectodea molleri chloragogenous tissue. In addition, we investigated their prospective use as biopriming agents to enhance Zea mays germination and seedling growth. Results were subjected to principal component analysis for potential correlations between the studied parameters. The bacterial strains displayed different in vitro plant growth-promoting characteristics and were efficient when applied in vivo as they significantly increased maize germination rate (26–78%), root elongation (67–84%), seedlings fresh weight and dry weight. Aeromonas encheleia TC22 was the most significant strain to influence germination due to its high ability to produce indole-3-acetic acid, and along with Pseudomonas azotoformans TC1, they were the most proficient at enhancing seedling root elongation and biomass, which was significantly correlated with their in vitro plant growth-promoting traits. Our findings indicate that isolates TC22 and TC1 are potent bio-primers for maize seeds and should be tested further for their use as biopriming inoculants.
Evaluation of filter paper as a means of transporting inactivated Gram-negative non-fermentative bacteria and Haemophilus spp. for identification using the MALDI-TOF MS system
Authors: Carneiro M.S. , Crispim M.N. , Wilhelm C.M. , Volpato F.C.Z. , Barth A.L. 
Published: March 2022

Abstract
This study aimed to evaluate the filter paper as a means to transport inactivated Gram-negative non-fermentative (GNNF) bacteria and Haemophilus spp. for analysis using MALDI-TOF MS. A total of 133 isolates were evaluated and the analysis of each isolate was performed directly from original bacterial colony and in filter paper after the processing. To evaluate the agreement between the identification performed directly from the colony and after impregnation in filter paper, we assign the scores: &gt;2·3 as excellent (E); 2·0 to 2·3 as very good (VG); 1·7–1·99 as good (G); &lt;1·7 as unidentified (U). The divergences were classified as: Minor Divergence, Intermediate Divergence and Major Divergence. A total of 80 isolates transported in the filter paper disks presented full category concordance; 39 isolates presented Minor Divergence; 4 isolates present Intermediate Divergence; 4 isolates present Major Divergence and 6 isolates present better results after impregnation in filter paper. The proposed methodology of bacteria transportation presented a sensitivity of 96·9% and a specificity of 100%. The filter paper as a means to transport and storage of inactivated GNNF and Haemophilus spp. may be considered a potential tool for faster, more accurate, biosafe and less-expensive identification.
Antimicrobial resistance tendency and collateral sensitivity of Staphylococcus aureus adapted to antibiotics or extracts of medicinal plants grown in Viet Nam
Authors: Nguyen T.-P. , Vu Thi N.-A. , Nguyen Diep X.-N. , Nguyen T.N. , Bui L.M. 
Published: March 2022

AbstractAntimicrobial resistance has silently turned into one of the biggest threats to global health, marking the fall of the Golden age of antibiotics. In the search for antibiotic replacement or enhancement, plant-derived natural compounds have attracted lots of interest. Even though firmly believed, the low-resistance tendency of pathogenic bacteria against plant extracts has been scarcely demonstrated. In this study, we investigated the antibacterial activities of diethyl ether extracts from six medicinal plants grown in Viet Nam against Staphylococcus aureus and its variants, which were in vitro adapted to the same extracts. After 30 passages of S. aureus growing under sub-lethal concentrations of plant extracts or antibiotics, the bacteria slowly adapted to the extracts while rapidly resisting the antibiotics. Most of the resulting strains obtained from the adaptation to plant extracts were collaterally sensitive to antibiotics. In contrast, antibiotic-adapted strains showed cross-resistance to both antibiotics and extracts. The findings provided evidence of the low-resistance tendency of S. aureus to antimicrobial plant extracts. It is the first time a collateral antibiotic sensitivity of S. aureus adapted to natural compounds has been observed, suggesting an alternative approach to fight antibiotic resistance.
Balthasar, the Sublime, and the Avant‐Garde
Authors: Fritz Peter Joseph 
Published: March 2022

AbstractFollowing Cyril O’Regan’s suggestion of possible Balthasarian reflections on the sublime and the avant‐garde, this article argues that actualizing these possibilities could contribute fruitfully to Hans Urs von Balthasar’s theological‐aesthetic resistance to Hegelian economy (mastery of all meaning and value through scientific reason) and to postmodern alternative philosophies to Hegel. The article expands the Balthasarian notion of “form” to open new partnerships between Balthasarian aesthetics and artistic currents that share his project of resisting economy. The article examines Balthasar’s early rejection of avant‐garde and modern art in historical and cultural context. It effects a critical dialogue between Balthasar and two prominent postmodern precedents for thinking about the sublime and avant‐garde art (Jean‐François Lyotard and Jean‐Luc Nancy). It constructs a different, Balthasarian perspective on the sublime and the avant‐garde, emphasizing the ontological sublime (Goethe, Scheeben) and Christus deformis (Augustine, Bonaventure). Then it sets the Balthasarian sublime in conversation with contemporary artist Sarah Sze to discover new counter‐economic possibilities for the Balthasarian Gestalt.
Modification of the N‐terminal FWKG−αH1 element of potyviral HC‐Pro affects its multiple functions and generates effective attenuated mutants for cross‐protection
Authors: Raja Joseph A. J. , Huang Chung‐Hao , Chen Chin‐Chih , Hu Wen‐Chi , Cheng Hao‐Wen , Goh Reun‐Ping , Chao Chia‐Hung , Tan Yue‐Rong , Yeh Shyi‐Dong 
Published: March 2022

AbstractControl of plant viruses by cross‐protection is limited by the availability of effective protective strains. Incorporation of an NIa‐protease processing site in the extreme N‐terminal region of the helper component protease (HC‐Pro) of turnip mosaic virus (TuMV) resulted in a mutant virus TuHNDI that induced highly attenuated symptoms. Recombination analysis verified that two variations, F7I mutation and amino acid 7‐upstream‐deletion, in HC‐Pro co‐determined TuHNDI attenuation. TuHNDI provided complete protection to Nicotiana benthamiana and Brassica campestris subsp. chinensis plants against infection by the severe parental strain. Aphid transmission tests revealed that TuHNDI was not aphid‐transmissible. An RNA silencing suppression (RSS) assay by agroinfiltration suggested the RSS‐defective nature of the mutant HC‐Pro. In the context (amino acids 3–17) encompassing the two variations of HC‐Pro, we uncovered an FWKG−α‐helix 1 (αH1) element that influenced the functions of aphid transmission and RSS, whose motifs were located far downstream. We further demonstrated that HC‐Pro F7 was a critical residue on αH1 for HC‐Pro functions and that reinstating αH1 in the RSS‐defective HC‐Pro of TuHNDI restored the protein's RSS function. Yeast two‐hybrid and bimolecular fluorescence complementation assays indicated the FWKG−αH1 element as an integral part of the HC‐Pro self‐interaction domain. The possibility of regulation of the mechanistically independent functions of RSS and aphid transmission by the FWKG−αH1 element is discussed. Extension of TuMV HC‐Pro FWKG−αH1 variations to another potyvirus, zucchini yellow mosaic virus, also generated nonaphid‐transmissible cross‐protective mutant viruses. Hence, the modification of the FWKG−αH1 element can generate effective attenuated viruses for the control of potyviruses by cross‐protection.
Neuropathological changes in advanced Parkinson’s disease
Authors: Adachi Tadashi , Hanajima Ritsuko 
Published: March 2022

AbstractPatients with advanced Parkinson’s disease (PD) show severe motor dysfunction, wearing‐off, dyskinesia, dementia, and autonomic failure. These symptoms correlate with the development of neuropathological findings such as severe nigrostriatal and non‐dopaminergic neuron loss and gliosis, and phosphorylated alpha‐synuclein (pSyn) accumulation in the central and peripheral nervous systems. In addition, there are often co‐pathological changes, including Alzheimer‐related tau and amyloid accumulations. According to Braak’s PD progression hypothesis, at the time of clinical diagnosis of PD, accumulation of pSyn‐positive structures is already widespread in the brainstem and peripheral nervous system, and in advanced PD, these pSyn‐positive structures have spread to the limbic system and neocortex. In this review, we describe the basic neuropathology of PD, with particular attention given to the Braak hypothesis and the distribution of pSyn‐positive structures in advanced PD.
The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO2‐dependent gene expression
Authors: Mantovani Oliver , Reimann Viktoria , Haffner Michael , Herrmann Felix Philipp , Selim Khaled A. , Forchhammer Karl , Hess Wolfgang R. , Hagemann Martin 
Published: March 2022

Summary

The amount of inorganic carbon (Ci) fluctuates in aquatic environments. Cyanobacteria evolved a Ci‐concentrating mechanism (CCM) that is regulated at different levels. The regulator SbtB binds to the second messengers cAMP or c‐di‐AMP and is involved in acclimation to low Ci (LC) in Synechocystis sp. PCC 6803. Here, we investigated the role of SbtB and of associated second messengers at different Ci conditions.

The transcriptome of wild‐type (WT) Synechocystis and the ΔsbtB mutant were compared with Δcya1, a mutant defective in cAMP production, and ΔdacA, a mutant defective in generating c‐di‐AMP.

A defined subset of LC‐regulated genes in the WT was already changed in ΔsbtB under high Ci (HC) conditions. This response of ΔsbtB correlated with a diminished induction of many CCM‐associated genes after LC shift in this mutant. The Δcya1 mutant showed less deviation from WT, whereas ΔdacA induced CCM‐associated genes under HC. Metabolome analysis also revealed differences between the strains, whereby ΔsbtB showed slower accumulation of 2‐phosphoglycolate and ΔdacA differences among amino acids compared to WT.

Collectively, these results indicate that SbtB regulates a subset of LC acclimation genes while c‐di‐AMP and especially cAMP appear to have a lesser impact on gene expression under different Ci availabilities.
Can 18‐years of data from a tertiary referral center help to identify risk factors in esophageal atresia?
Authors: Narsat Mehmet Ali , Kılıç Şeref Selçuk , Özden Önder , Alkan Murat , Tuncer Recep , İskit Hilmi Serdar 
Published: March 2022

AbstractBackgroundEsophageal atresia is a complex esophageal malformation with an incidence of 1 in 3,500–4,000 live births, and it usually occurs together with anomalies in other systems or chromosomes. This study aimed to investigate the short‐term and long‐term results of cases of esophageal atresia retrospectively in our institution and to analyze the factors affecting the outcome.MethodsCharts of the patients managed for esophageal atresia in our tertiary pediatric surgery department were investigated retrospectively. Statistical analysis was performed to determine the risk factors for morbidity and mortality.ResultsOne hundred and thirteen (95.8%) of 118 cases underwent a single‐stage or staged esophagoesophagostomy procedure. In only five of the 40 patients with a long gap between the two atretic ends was an esophageal replacement procedure required. The most common early and late complications were anastomotic stenosis (41.6%) and gastroesophageal reflux (44.9%). In logistic regression analysis, the birthweight (OR [95% CI] = 0.998 [0.997, 0.999], P = 0.001) and preoperative inotrope requirement (OR [95% CI] = 13.8 [3.6–53.3], P < 0.001) were the two risk factors in the mortality prediction model obtained by multivariate analysis. The gap length between the two atretic ends (OR [95% CI] = 1.436 [1.010, 2.041], P = 0.044) and the number of sutures for anastomosis (OR [95% CI] = 1.313 [1.042, 1.656], P = 0.021) were the two risk factors in the gastroesophageal reflux prediction model obtained by multivariate analysis.ConclusionsOur study's early and late complication rates were like those found in other studies. Identifying risk factors would be beneficial and might help reduce the severity of potential complications in esophageal atresia patients. Prospective studies on large patient series would help develop registry‐based, standardized management protocols.