bluuebunny/crossref_metadata_2025_split huggingface dataset:
The interface stiffness and topographic feature dictate interfacial invasiveness of cancer spheroids
Authors: Mao Bin-Hsu , Nguyen Thi Kim Mai , Tang Ming-Jer , Kamm Roger D , Tu Ting-Yuan 
Published: December 2022

Abstract
During cancer metastasis, tumor cells likely navigate, in a collective manner, discrete tissue spaces comprising inherently heterogeneous extracellular matrix microstructures where interfaces may be frequently encountered. Studies have shown that cell migration modes can be determined by adaptation to mechanical/topographic cues from interfacial microenvironments. However, less attention has been paid to exploring the impact of interfacial mechnochemical attributes on invasive and metastatic behaviors of tumor aggregates. Here, we excogitated a collagen matrix-solid substrate interface platform to investigate the afore-stated interesting issue. Our data revealed that stiffer interfaces stimulated spheroid outgrowth by motivating detachment of single cells and boosting their motility and velocity. However, stronger interfacial adhesive strength between matrix and substrate led to the opposite outcomes. Besides, this interfacial parameter also affected the morphological switch between migration modes of the detached cells and their directionality. Mechanistically, myosin II-mediated cell contraction, compared to matrix metalloproteinases-driven collagen degradation, was shown to play a more crucial role in the invasive outgrowth of tumor spheroids in interfacial microenvironments. Thus, our findings highlight the importance of heterogeneous interfaces in addressing and combating cancer metastasis.
Extracellular-matrix-mimicked 3D nanofiber and hydrogel interpenetrated wound dressing with a dynamic autoimmune-derived healing regulation ability based on wound exudate
Authors: Gao Liheng , Liu Xingxing , Zhao Wenshuo , Li Chaojin , Wang Fujun , Gao Jing , Liao Xinqin , Wei Lei , Wu Hao , Zheng Yuanjin , Wang Lu 
Published: December 2022

Abstract
Dynamic regulation of wound physiological signals is the basis of wound healing. Conventional biomaterials delivering growth factors to drive wound healing leads to the passive repair of soft tissues because of the mismatch of wound healing stages. Meanwhile, the bioactivity of wound exudate is often restricted by oxidation and bacterial contamination. Herein, an extracellular matrix mimicked nanofiber/hydrogel interpenetrated network (NFHIN) was constructed with a 3D nanofibrous framework for cell immigration, and interfiled aerogel containing cross-linked hyaluronic acid and hyperbranched polyamidoamine to balance the wound microenvironment. The aerogel can collect wound exudate and transform into a polycationic hydrogel with contact-killing effects even against intracellular pathogens (bactericidal rate > 99.9% in 30 min) and real-time scavenging property of reactive oxygen species. After co-culturing with the NFHIN, the bioactivity of fibroblast in the ex vivo blister fluid was improved by 389.69%. The NFHIN showed sustainable exudate management with moisture–vapor transferring rate (6000 g m−2 ×24 h), equilibrium liquid content (75.3%), Young’s modulus (115.1 ± 7 kPa), and anti-tearing behavior similar to human skin. The NFHIN can collect and activate wound exudate, turning it from a clinical problem to an autoimmune-derived wound regulation system, showing potential for wound care in critical skin diseases.
Design of 3D-printed universal oral stent for tongue immobilization in head and neck radiotherapy
Authors: Mundee Thunpisit , Jongwannasiri Chavin , Fuangrod Todsaporn 
Published: December 2022

Abstract
The primary treatment for head and neck cancer is radiotherapy, which can cause complications and effects, such as the ability to speak, taste, produce saliva, and swallow. An oral stent is an immobilization tool for minimizing the dose in the tongue (or hard palate) by locking the tongue position during radiation delivery. It can improve the treatment accuracy due to less uncertainty caused by tongue position uncertainty between treatment fractions. However, commercial oral stents are not widely adopted in developing countries due to their unaffordable price. This study aimed to design the universal oral stent (UOS) to achieve high efficiency, ease to use, and low-cost fabrication based on 3D printing technology. There were five experiments to evaluate the UOS design and fabrication versus the modified cut syringe, including finite element analysis (FEA), the usability test, the micro Vickers hardness test, single beam dose analysis, and dose calculation on treatment plan simulations. The proposed UOS design and fabrication presented a high capability to apply for clinical use.
The automated measurement of CT number linearity using an ACR accreditation phantom
Authors: Anam Choirul , Amilia Riska , Naufal Ariij , Budi Wahyu S , Maya Anisa T , Dougherty Geoff 
Published: December 2022

Abstract
We developed a software to automatically measure the linearity between the CT numbers and densities of objects using an ACR 464 CT phantom, and investigated the CT number linearity of 16 different CT scanners. The software included a segmentation-rotation method. After segmenting five objects within the phantom image, the software computed the mean CT number of each object and plotted a graph between the CT numbers and densities of the objects. Linear regression and coefficients of regression, R2, were automatically calculated. The software was used to investigate the CT number linearity of 16 CT scanners from Toshiba, Siemens, Hitachi, and GE installed at 16 hospitals in Indonesia. The linearity of the CT number obtained on most of the scanners showed a strong linear correlation (R
2 > 0.99) between the CT numbers and densities of the five phantom materials. Two scanners (Siemens Emotion 16) had the strongest linear correlation with R
2 = 0.999, and two Hitachi Eclos scanners had the weakest linear correlation with R
2 < 0.99.
Comparison of Clinical Characteristics Between Patients With Altitude-Related Hypertension and Essential Hypertension at High Altitude
Authors: Wang Xiao-zhou , Bian Hui-ping , Yang Lei , Xu Xiao-long , Bai Jie , Han Qiong , Yan Ping , Zhang Jing , Zhou Qian , Zhao Cai-lian , Duo Jie , Ma Zhan-qing , Zhang Wei 
Published: December 2022

Abstract

Background
To investigate the clinical characteristics of essential hypertension (EH) and altitude-related hypertension (ARH) hospitalized in Qinghai, and to provide the relevant information for the prevention and treatment of ARH.

Methods
A total of 1,987 hypertensive patients from 3.0 to 4.5 km altitude areas, and hospitalized in Qinghai Cardio-Cerebrovascular Hospital were enrolled. Among them, 165 patients with elevated blood pressure (BP) (systolic BP ≥140 and/or diastolic BP ≥90 mm Hg) at high altitude and normal BP or BP reduction &gt;20/10 mm Hg 2 weeks after arriving at Xining (altitude 2.26 km) were defined as ARH.

Results
Compared with EH, ARH patients at higher altitude had earlier onset age (48.0 ± 11.5 vs. 54.4 ± 9.8 years, P &lt; 0.001), higher percentage of male patients (77.6% vs. 55.3%, P &lt; 0.001), higher diastolic BP (115.8 ± 18.5 vs. 108.6 ± 15.4 mm Hg, P &lt; 0.001), and lower systolic BP (165.9 ± 21.3 vs. 178.9 ± 24.4 mm Hg, P &lt; 0.001). Erythrocytes, hemoglobin, hematocrit, and uric acid levels were higher in ARH patients than in EH patients (P &lt; 0.05). Echocardiography showed that the right atrial and ventricular diameters were larger in ARH than EH (P &lt; 0.05). The proportion of pulmonary artery hypertension in ARH was higher than EH (9.1% vs. 4.9%, P = 0.020), but there was no significant difference in the target organ damage and clinical complications of hypertension between the 2 groups. Logistic regression analysis showed that male, Han nationality, high-altitude living, elevated hemoglobin and uric acid, and obesity were associated with the occurrence of ARH.

Conclusions
ARH is prevalent in young and middle-aged men, and is associated with Han nationality, elevated hemoglobin, uric acid, and obesity.
Accuracy of vancomycin AUC values estimated with trough-only data in a veteran population
Authors: Fewel Nathan 
Published: December 2022

AbstractPurposeThe purpose of this study was to evaluate the accuracy and precision of estimating area under the curve (AUC) values using only vancomycin trough concentrations versus both peak and trough values derived from applying 4 different volume of distribution (Vd) models in a veteran population.MethodsThis retrospective, observational study was performed from July 2021 to April 2022 using data from 5 Veterans Affairs hospitals across the US. AUC values for a total of 259 veterans were included in the analysis, with 10 excluded after pooling of data. Trough-only AUC values were calculated with 1-compartment intermittent infusion equations (Sawchuk-Zaske equations) using age- and weight-adjusted Vd values derived from an online calculator (VancoPK) or fixed Vd values specified by 3 comparator models.ResultsThe mean population peak-trough AUC was 496 (range, 266-886). Of the 4 Vd models evaluated, the VancoPK model was the most accurate and precise, yielding a mean trough-only AUC of 491, with a correlation of 0.925; the root mean square error was 41, meaning that approximately 95% of the trough-only AUCs were within 82 points of values calculated using AUC peak-trough couplets.ConclusionA trough-only AUC estimation approach has many advantages over a peak-trough approach. The equation Vd = 0.29 (age) + 0.33 (actual BW in kg) + 11 provided accurate and precise AUC estimates with trough-only data when applied to pharmacokinetic equations in a veteran population.
Treatment-experienced patient with Roux-en-Y gastric bypass successfully treated with sofosbuvir/velpatasvir/voxilaprevir: A case report
Authors: Mod Alexis Taylor , Katz Rivka 
Published: December 2022

AbstractPurposeThe purpose of this article is to describe a patient with chronic hepatitis C virus (HCV) infection, who had previously taken 2 other direct-acting antiviral (DAA) regimens and undergone Roux-en-Y gastric bypass (RYGB), successfully treated with a sofosbuvir/velpatasvir/voxilaprevir regimen.SummaryA 64-year-old, African American male presented for management of HCV genotype 1a infection after 2 failed courses of treatment. The patient had a history of severe gastroesophageal reflux disease (GERD), and he was unable to discontinue proton pump inhibitor (PPI) use during all treatment regimens. He was originally treated with 12 weeks of ledipasvir/sofosbuvir but relapsed 3 to 4 months post treatment. The patient had undergone RYGB for the treatment of obesity. After surgery, the patient was re-treated with sofosbuvir/velpatasvir plus ribavirin for 24 weeks. The patient again relapsed 3 months after completing therapy. At our clinic, the patient was started on sofosbuvir/velpatasvir/voxilaprevir for 12 weeks. The patient decided on his own to chew the medication, as he was concerned about medication absorption post surgery, and took concurrent once-daily esomeprazole. Confirmatory posttreatment laboratory work showed his HCV RNA viral load remained undetectable, indicating sustained virologic response, and the patient was deemed cured of HCV.ConclusionA patient with a history of relapse after previous DAA therapy and RYGB was successfully treated for HCV infection with sofosbuvir/velpatasvir/voxilaprevir tablets, which he chewed. This case report shows sofosbuvir/velpatasvir/voxilaprevir may be an effective treatment option in the RYGB population, although additional research is needed.
Difference in Perception of Nasal Aesthetic Appearance Between Surgeons and Rhinoplasty Patients: A Single-Center Perspective
Authors: Kim Cherine H , Kandathil Cherian K , Saltychev Mikhail , Youn Gun Min , Shah Jay P , Khan Suleman Imran , Buba Cibele Madsen , Okland Tyler S , Wei Eric X , Fullerton Zoe , Most Sam P 
Published: December 2022

Abstract

Background
Patient satisfaction is an essential outcome measure after a rhinoplasty. Yet it is not known whether the opinions of rhinoplasty patients and surgeons on nasal aesthetic appearance differ.

Objectives
The aim of this study was to determine the differences between patients and surgeons in their perception of nasal aesthetic appearance.

Methods
A retrospective cohort of 300 patients seen in consultation for cosmetic, functional, or combined cosmetic and functional rhinoplasty at a single tertiary care center from June 2017 to June 2020 was studied. Based on preoperative patient images, 6 surgeons with varying levels of expertise assessed nasal aesthetics utilizing a modified Standardized Cosmesis and Health Nasal Outcomes Survey for nasal cosmesis (SCHNOS-C). These scores were then compared to the patient-reported SCHNOS-C scores.

Results
The cosmetic, functional, and combined subgroups consisted of 100 patients each. The mean [standard deviation] age was 35.4 [13.7] years and 64% were women. The modified SCHNOS-C scores were well-correlated among the 6 surgeons but showed only weak correlations of 0.07 to 0.20 between patient-reported scores and scores assessed by the surgeons. Compared with the surgeon's scores, patients in the cosmetic subgroup perceived their nasal aesthetic problems to be more severe whereas the those in the functional subgroup perceived their nasal aesthetic problems to be milder compared with the surgeons’ assessment.

Conclusions
Our findings suggest that patients and surgeons perceive nasal cosmesis differently. This difference should be considered carefully when planning rhinoplasty or assessing its outcome.
Synthetic Reabsorbable Mesh (GalaFLEX) as Soft Tissue Adjunct in Breast Augmentation Revision Surgery
Authors: Buccheri Ernesto Maria , Villanucci Amedeo , Mallucci Patrick , Bistoni Giovanni , de Vita Roy 
Published: December 2022

Abstract

Background
A significant number of patients with a previous submuscular breast augmentation require a revision surgical procedure during their long-term follow-up period that involves changing the implant pocket from submuscular to subglandular. Reduced soft tissue thickness represents a major concern, discouraging surgeons from undertaking this approach.

Objectives
The aim of this study was to determine whether a synthetic resorbable mesh used as a supplemental covering device was safe and yielded improved soft tissue thickness and protection against capsular contracture in patients undergoing implant plane shift to subglandular.

Methods
A retrospective review of 34 patients who underwent revision of a previous breast augmentation involving implant change in a subglandular pocket and upper pole GalaFLEX (Galatea Surgical, BD; Franklin Lakes, NJ) mesh coverage was conducted. Onset of minor and major complications was recorded during short- and medium-term follow-up. Patient satisfaction was assessed via an anonymous questionnaire administered 6 months postoperatively.

Results
During the follow-up period, which ranged from a minimum of 6 months to a maximum of 28 months, no minor or major complications were reported. In particular, no wrinkling/rippling or significant capsular contracture onset was observed. High levels of patient satisfaction were determined, according to self-reporting questionnaires.

Conclusions
The initial results of this preliminary report from this case series demonstrate the safety of GalaFLEX as a supplemental device in terms of improved soft tissue coverage of the implant for complex revisional cases in breast augmentation.

Level of Evidence: 4
The Efficacy and Associated Learning Curve of Office-Based High-Resolution Ultrasound to Detect Shell Failure in Breast Implants
Authors: Ferenz Sarah , McGuire Patricia , Glicksman Caroline 
Published: December 2022

Abstract

Background
High-resolution ultrasound (HRUS) is widely employed in plastic surgery practices to detect implant rupture prior to revisional surgery. Published research has found a good overall accuracy of shell failure detection. The literature often references a learning curve associated with incorporating this imaging technique into a medical practice, but it has yet to be visualized or defined.

Objective
This study was undertaken to calculate current testing statistics for use of HRUS to detect shell failure and to define the learning curve associated with the predicted improvement reflected by statistics of test and surgeon proficiency.

Methods
A retrospective review of sequential in-office HRUSs on patients with breast implants was conducted across 2 plastic surgery practices. Preoperative ultrasound reports and intraoperative findings were compared. Test statistics were calculated for each group of 10 subsequent patients, and a regression analysis was performed to define the learning curve.

Results
A total of 480 implants were examined and averages for all test statistics were calculated. All were higher than most of the previously reported literature standards. The regression analysis showed a linear improvement for both sensitivity and specificity over time, with significant improvement in sensitivity.

Conclusion
Results show that HRUS is highly effective in detecting shell failure in breast implants. There is also a calculable linear improvement for all test values of the HRUS over time. Surgeons were able to remain above the literature standard for sensitivity after their 60th HRUS reading. This improvement shows that continued use and practice of the imaging technique allow for more accurate findings.
Existence of biogeographic barriers for the long-term Neogene–Quaternary divergence and differentiation ofKoenigia forrestiiin the Himalaya–Hengduan Mountains
Authors: Rana Hum Kala , Rana Santosh Kumar , Luo Dong , Sun Hang 
Published: December 2022

AbstractThe prolonged interplay between orographic and climatic changes creates biogeographic barriers, resulting in the allopatric differentiation of plants in the Himalaya–Hengduan Mountains. Such consequences have led us to investigate the long-term Neogene–Quaternary geo-climatic history of the Eastern Himalaya–Hengduan Mountains. Narrowly distributed populations of Koenigia forrestii were sampled (ten populations, 97 individuals) and analysed for their genetic architecture, including phylogenetic reconstruction (based on plastome and plastid DNA/nuclear regions), molecular dating and demography, in combination with niche dynamics. We estimated that K. forrestii (stem age: 11.39 Mya) diverged into three non-overlapping distributed lineages during the Neogene–Quaternary periods (5.84–2.57 Mya), with Eastern Himalaya (EHa) being the first and most diverse lineage. ‘Isolation by environment’ revealed the existence of genetic structures that were significantly affected by the disparate environment. The presence of demographic events is well supported by the Bayesian skyline plot, indicating recent demographic expansion. Hence, lineage divergence and differentiation were mainly triggered by the heterogeneous environment associated with the biogeographic barriers due to the Tsangpo-Brahmaputra Grand Canyon, Mekong-Salween Divide and local mountain systems. Nevertheless, niche shift and local adaptation are the keys to determining the genetic architecture, demographic dynamics and diversification history of K. forrestii.
Disturbed hippocampal intra-network in first-episode of drug-naïve major depressive disorder
Authors: Watanabe Keita , Okamoto Naomichi , Ueda Issei , Tesen Hirofumi , Fujii Rintaro , Ikenouchi Atsuko , Yoshimura Reiji , Kakeda Shingo 
Published: December 2022

AbstractComplex networks inside the hippocampus could provide new insights into hippocampal abnormalities in various psychiatric disorders and dementia. However, evaluating intra-networks in the hippocampus using MRI is challenging. Here, we employed a high spatial resolution of conventional structural imaging and independent component analysis to investigate intra-networks structural covariance in the hippocampus. We extracted the intra-networks based on the intrinsic connectivity of each 0.9 mm isotropic voxel to every other voxel using a data-driven approach. With a total volume of 3 cc, the hippocampus contains 4115 voxels for a 0.9 mm isotropic voxel size or 375 voxels for a 2 mm isotropic voxel of high-resolution functional or diffusion tensor imaging. Therefore, the novel method presented in the current study could evaluate the hippocampal intra-networks in detail. Furthermore, we investigated the abnormality of the intra-networks in major depressive disorders. A total of 77 patients with first-episode drug-naïve major depressive disorder and 79 healthy subjects were recruited. The independent component analysis extracted seven intra-networks from hippocampal structural images, which were divided into four bilateral networks and three networks along the longitudinal axis. A significant difference was observed in the bilateral hippocampal tail network between patients with major depressive disorder and healthy subjects. In the logistic regression analysis, two bilateral networks were significant predictors of major depressive disorder, with an accuracy of 78.1%. In conclusion, we present a novel method for evaluating intra-networks in the hippocampus. One advantage of this method is that a detailed network can be estimated using conventional structural imaging. In addition, we found novel bilateral networks in the hippocampus that were disturbed in patients with major depressive disorders, and these bilateral networks could predict major depressive disorders.
Hydrogels for the management of second-degree burns: currently available options and future promise
Authors: Cook Katherine A , Martinez-Lozano Edith , Sheridan Robert , Rodriguez Edward K , Nazarian Ara , Grinstaff Mark W 
Published: December 2022

Abstract
Burn wounds result from exposure to hot liquids, chemicals, fire, electric discharge or radiation. Wound severity ranges from first-degree injury, which is superficial, to fourth-degree injury, which exposes bone, tendons and muscles. Rapid assessment of burn depth and accurate wound management in the outpatient setting is critical to prevent injury progression into deeper layers of the dermis. Injury progression is of particular pertinence to second-degree burns, which are the most common form of thermal burn. As our understanding of wound healing advances, treatment options and technologies for second-degree burn management also evolve. Polymeric hydrogels are a class of burn wound dressings that adhere to tissue, absorb wound exudate, protect from the environment, can be transparent facilitating serial wound evaluation and, in some cases, enable facile removal for dressing changes. This review briefly describes the burn level classification and common, commercially available dressings used to treat second-degree burns, and then focuses on new polymeric hydrogel burn dressings under preclinical development analyzing their design, structure and performance. The review presents the follow key learning points: (1) introduction to the integument system and the wound-healing process; (2) classification of burns according to severity and clinical appearance; (3) available dressings currently used for second-degree burns; (4) introduction to hydrogels and their preparation and characterization techniques; and (5) pre-clinical hydrogel burn wound dressings currently being developed.