bluuebunny/crossref_metadata_2025_split huggingface dataset:
The ratio of CT attenuation values of the ureter above and below ureteral stones is a useful preoperative factor for predicting impacted ureteral stones
Authors: Deguchi Ryusuke , Yamashita Shimpei , Iwahashi Yuya , Muraoka Satoshi , Kikkawa Kazuro , Kohjimoto Yasuo , Hara Isao 
Published: May 2022

Abstract
IntroductionImpacted stones typically make it difficult to perform ureteroscopic lithotripsy (URSL), so it is useful to preoperatively predict such impaction. We focused on CT attenuation values of the ureter above and below the stone (‘HU above’ and ‘HU below’) and calculated their ratio (HAB ratio). The aim was to investigate whether HAB ratio could predict impacted stones preoperatively. Material and MethodsBetween 2011 and 2019, 171 patients from our hospital that had URSL for ureteral stones with pretreatment non-contrast computed tomography (NCCT) were retrospectively identified. Ureteral wall thickness (UWT), ureteral wall volume (UWV) and HAB ratio were recorded. HAB ratio was calculated as ‘HU below’ divided by ‘HU above’. Impacted stones were defined as fixed stones that did not move by means of ureteroscopic manipulation or water pressure. ResultsOf the 171 procedures, 46 (27%) involved patients with impacted stones. Comparing patient characteristics and stone parameters according to impaction status, factors with significant difference included grade of hydronephrosis, UWT, and HAB ratio (all P<0.01). Multivariate analysis indicated that significant independent predictors of impacted stones were thicker UWT and higher HAB ratio (all P<0.01).ConclusionsHAB ratio was a significant preoperative predictor of stone impaction in patients undergoing URSL for ureteral stones. HAB ratio may be useful for selecting the treatment and preoperative preparations.
Bioelectronic Screening Platform for Real-Time Monitoring of Tumour-derived Extracellular Vesicle-Induced Epithelial-to-Mesenchymal Transition
Authors: Traberg Walther , Uribe Johana , Druet Victor , Hama Adel , Moysidou Chrysanthi-Maria , Huerta Miriam , McCoy Reece , Hayward Daniel , Savva Achilleas , Genovese Amaury , Pavagada Suraj , Lu Zixuan , Koklu Anil , Pappa Anna-Maria , Fitzgerald Rebecca , Inal Sahika , Daniel Susan , Owens Roisin 
Published: May 2022

Abstract
Tumour-derived extracellular vesicles (TEVs) induce the epithelial-to-mesenchymal (EMT) in non-malignant cells to promote invasion and cancer metastasis. As such, TEVs represent a novel therapeutic target in a field severely lacking in efficacious anti-metastasis treatments. However, scalable technologies that allow continuous, multiparametric monitoring of therapeutic response for identifying metastasis inhibitors are missing. Here, we report the development of a platform based on organic electrochemical transistors (OECTs) for the real-time monitoring of TEV-induced EMT and screening of anti-metastatic drugs. We used TEVs derived from the triple-negative breast cancer (TNBC) cell line MDA-MB-231, to induce EMT in non-malignant breast epithelial cells (MCF10A) over a 9-day period, recapitulating a model of invasive ductal carcinoma metastasis. We performed extensive biological validation using immunofluorescence (IF) imaging and protein expression analysis, providing mechanistic insight using an epigenetics approach, and demonstrate our ability to obtain multiparametric functional readouts of cells using OECTs. Further, by employing OECT-based phenotypic drug screening, we identify heparin as an effective blocker of TEV-induced EMT in vitro, showcasing a promising anti-metastatic drug.
Analysis of N7-(2-carbamoyl-2-hydroxyethyl)guanine in Dried Blood Spot after Food Exposure by Ultra High Perfomance Liquid Chromatography – Tandem Mass Spectrometry
Authors: Haharap Yahdiana , Safitri Winning Bekti , Sunarsih Sunarsih 
Published: May 2022

Abstract
N7-(2-carbamoyl-2-hydroxyethyl)guanine (N7-GAG) is a DNA adduct formed by glycidamide, which is the metabolite of acrylamide. Acrylamide can be found in foods containing reducing sugars and asparagine that are heated at high temperatures. Analysis of N7-(2-carbamoyl-2-hydroxyethyl)guanine was perfomed in Dried Blood Spot (DBS) samples from 25 subjects of group test who consumed a lot of acrylamide-containing foods and 25 subjects of negative control group. This study aimed to determine whether there is a significant difference in the levels of N7-(2-carbamoyl-2-hydroxyethyl)guanine between the two groups. DBS samples were extracted using the QIAamp DNA Mini Blood Kit and analyzed using Ultra High Performance Liquid Chromatography – Tandem Mass Spectrometry (UHPLC-MS/MS). Separation was performed using an Acquity UPLC BEH C18 column (2.1 mm x 100 mm; 1.7 µm), eluted a flow rate of 0.1 ml/minute under an isocratic of mobile phase of 0.1% formic acid and acetonitrile. The bioanalytical method of N7-(2-carbamoyl-2-hydroxyethyl)guanine in DBS with allopurinol as the internal standard by using UHPLC-MS/MS has been validated. The calibration curve range of N7-(2-carbamoyl-2-hydroxyethyl)guanine obtained was 10–300 ng/ml with a coefficient of correlation of 0.997. The results of the analysis on 25 test group subjects showed that the concentration of N7-(2-carbamoyl-2-hydroxyethyl)guanine ranged from 1.87 to 23.71 ng/ml, while the 25 subjects in the negative group ranged from 1.18 to 8.47 ng/ml. The results of the Mann Whitney test showed that there was a significant difference in the levels of N7-(2-carbamoyl-2-hydroxyethyl)guanine between the test group and the negative control group with p value less than 0.001.
Identification and characterization of Allium cepa potential intron polymorphism markers
Authors: Jayaswall Kuldip , Sagar R. , Jayaswal Deepanshu , Kumar Akash , Singh Surendra Pratap , Seth Romit ,   Himanshu Sharma6, Mahajan Vijay , Kumar Sanjay , Singh Major 
Published: May 2022

Abstract

Allium cepa is a widely grown crop for its spice and culinary properties. For molecular breeding of Allium cepa and its improvement, mining and utilization of various sequence-based markers have been widely reported.Unfortunately, none has reported its molecular breeding using potential intron polymorphism (PIP) markers. Due to advantage of intron positioning prediction and practical utility, the PIP markers could be superior over the other molecular markers. These essential attributes, necessitated to establish an experiment for screening the possible PIP for polymorphism and cross-transferability in the Alliums. The screening results showed that among 500 Allium cepa potential intron polymorphism (AcPIP) markers, 275 are cross-transferable and polymorphic. Among the 275 AcPIPs, 111 polymorphic markers were physically mapped on to1st to 8th chromosomes of the Allium cepa. Out of the 275 AcPIP containing functionally significant markers, 118 were mapped in onion with arabidopsis proteome. BLAST analysis of 275 AcPIP containing expressed sequence tags (ESTs) showed their tolerance function to the large number of biotic and abiotic stresses. A set of 10 Alliums including cultivated Allium cepa (5), Allium sativum (3), and wild Alliums (2) were utilized to interpret the polymorphic possibilities of the 500 AcPIP markers. In totality, 55 percent AcPIP markers were polymorphic and cross-transferable among the Alliums. Across the 275 screened AcPIP markers, the highest percentage (92.7%) cross-transferability was found in Allium sativum var. Bhima Purple while it was lowest (82.1%) in the Allium fistulosum with 86.2% average cross transferability. The polymorphic information content (PIC) of AcPIP markers ranged from 0.03 to 0.47. Heterozygosity index (H) varied between 0.16 and 0.80. The constructed phylogenetic tree based on the AcPIP markers of 10 Alliums revealed different clusters due to differences in their taxonomic positions. Out of 275AcPIP markers, 10 AcPIP markers were selected for genotyping of 96 individuals of Allium cepa. Bayesian model-based structure and Jaccard dissimilarity-based NJ tree used for grouping of 96 individuals of Allium cepa showed two distinct groups (high total soluble solid; HTSS and low total soluble solid; LTSS), hence confirmed the reliabilities of these AcPIP markers. DNA fingerprint of Alliums with 20AcPIP markers suggested geographical reach of the released onion varieties and further validated the relevance of these 275 AcPIP markers. The results suggest that the 275 AcPIP validated functional molecular markers may be useful for the understanding of population structure, genomic association and comparative genomics of the Alliums for accelerating the breeding programme of the Alliums and other species.
Microclimate and animal thermal comfort indexes in different silvopastoral system arrangements in Caatinga
Authors: Neto Clemente Fernandes Santos , Silva Rodrigo Gregório , Maranhão Samuel Rocha , Torres Antônia Flávia Fernandes , Filho José Antonio Delfino Barbosa Barbosa , Macedo Vitor Hugo Maués , Cândido Magno José Duarte 
Published: May 2022

Abstract
The objective was to evaluate the microclimate and animal thermal comfort indexesin silvopastoral system in the Brazilian semiarid region with different woody cover levels within Caatinga trees. The experiment was carried out at the Teaching, Research and Extension Unit of the Federal Institute of Science and Technology of Ceará, Campus Limoeiro do Norte, in Chapada do Apodi, CE, in 2018. The treatments were: forage grown under woody cover of 46.15% (SS46), forage grown under woody cover of 30.00% (SS30) and forage grown under woody cover of 17.64% (SS18). The evaluations were carried out during three consecutive days, in the vegetative, transition and dormancy periods at 6 am; 10 am; 2 pm and 6 pm. First, the microclimate variables: relative air humidity (%), wind speed (m s -1 ), dry bulb temperature (°C), wet bulb temperature (°C), black globe temperature (°C), and dew point temperature (°C) were obtained for subsequent calculation of black globe temperature and humidity index and radiant thermal load (W m -2 ). SS46, SS30 and SS18 systems had no differences of black globe temperature and humidity index and radiant thermal load in the sun. There were significant reductions in black globe temperature and humidity index and radiant thermal load within the trees promoting improvement in the thermal environment at times of higher radiation load. The trees promoted better conditions of animal thermal comfort in all silvopastoral arrangements and periods of the year.
Distribution Function Based- Arithmetic Optimization Algorithm for Global optimization and Engineering Applications
Authors: Ates Abdullah , Abualigah Laith , Elaziz Mohamed Abd 
Published: May 2022

Abstract
In this study, Modified Arithmetic Optimization Algorithm (MAO) is proposed by updating the basic arithmetic optimization (AO) algorithm with different random distribution functions. The AO algorithm is a stochastic swarm-based algorithm that uses main mathematics operators (multiplication, division, subtraction, and addition) during the updating process. In the basic AO algorithm, random coefficients derived according to uniform distribution are used, especially in the generation of the initial population, exploration, and exploitation phases. In this study, these random coefficients are updated with Chi-Square, gamma, logistic, half normal, exponential, normal, extreme value, inverse Gaussian distribution functions. The efficacy of the developed MAO is evaluated using a set of experimental series including global benchmark optimization and real engineering applications named 3 DOF Hover flight system. For global optimization, the proposed MAO algorithm was run according to 100, 500, and 1000 dimensions for 23 different benchmark functions, and the results are compared with each other. As can be seen from the results, the proposed method produced better results than the classical AO results and well-known metaheuristic techniques. It is seen that MAO performs much better, especially in cases where the number of dimensions’ increases. In addition, 3 DOF Hover Experiment sets, which is an important problem in flight control systems, were used for the engineering application of the proposed method. Linear Quadratic Regulator (LQR) control structure is used to control this experiment set. In the LQR control structure, the Q and R matrices must be optimal. A total of 10 parameters were optimized, and the results were compared with Darwinian particle swarm optimization, fractional-order Darwinian particle swarm optimization, and classical AO algorithms. For comparison, first of all, optimization has been made on the simulation model of the system. As a result of this optimization, it was determined that the results of the MAO algorithm optimized according to the half-normal and exponential distribution functions have better control performance. Then, the optimization parameters obtained for the simulation model were tested in real-time 3 DOF Hover systems and it was shown that the results found work in real-time 3 DOF Hover systems.
Association between triglyceride–glucose index and nonalcoholic fatty liver disease in type 2 diabetes mellitus
Authors: Li Wei , Wang Yan , He Feng , Liu Zhuo , Dong Jie , Zhang Yuqi , Li Tianfang , Chen En , Liu Shengyun 
Published: May 2022

Abstract
Background
Lipid and glucose metabolism abnormalities are associated with nonalcoholic fatty liver disease (NAFLD). The triglyceride–glucose (TyG) index is a recently developed indicator that can identify individuals at risk for NAFLD. However, the applicability of the TyG index for identifying NAFLD in patients with type 2 diabetes mellitus (T2DM) is unclear. The aim of this study was to investigate the ability of the TyG index to identify individuals at risk for NAFLD in the T2DM population.
Methods
A total of 2280 participants with T2DM were recruited in this cross-sectional study. The TyG index was calculated, and NAFLD was diagnosed by ultrasonography. Binary logistic regression models were used to evaluate the association of the TyG index, glycemic parameters and lipid parameters with NAFLD.
Results
Logistic regression analysis showed that the TyG index was significantly associated with NAFLD in subjects with T2DM, the odds ratio (OR) were 3.27 (95% confidence interval [CI], 2.03–5.27; P < 0.001) for NAFLD in the highest TyG quartile after adjustment for known confounders. In stratified analysis, an elevated TyG index were more remarkably associated with NAFLD in younger patients (< 65 years; OR, 2.35; 95% CI, 1.83–3.02; P < 0.001), females (OR, 2.69; 95% CI, 1.67–4.32; P < 0.001), patients with BMI < 25 kg/m2 (OR, 2.80; 95% CI, 2.01–3.91; P < 0.0001), and with lower high-density lipoprotein cholesterol (< 1 mmol/L; OR, 2.76; 95% CI, 1.98–3.83; P < 0.001).
Conclusion
The TyG index is remarkably associated with NAFLD and shows superior ability for identify NAFLD risk compared with other lipid and glycemic parameters in the T2DM population.
New species and records of Leptusa from the Georgian Caucasus (Coleoptera: Staphylinidae: Aleocharinae)
Authors: Assing Volker 
Published: May 2022

Three field trips to West Georgia conducted in 2021 yielded 923 specimens of Leptusa Kraatz, 1858 belonging to ten species, two of which are described and illustrated: Leptusa (Neopisalia) pugiofera spec. nov. (Georgia: Kvemo Svaneti); L. (N.) insculpta spec. nov. (Georgia: Zemo Svaneti). The distributions of five species are mapped. The Leptusa fauna of the Caucasus region sensu lato is now represented by a total of 40 species, 21 of which (11 exclusive) have been recorded from Georgia. The Palaearctic fauna includes 420 species and 74 subspecies in 71 subgenera.
Taxonomic acts
Leptusa pugiofera spec. nov. – urn:lsid:zoobank.org:act:65DB13D7-F5EB-4C7A-816A-6C3891641F7A Leptusa insculpta spec. nov. – urn:lsid:zoobank.org:act:CE43945E-ABB8-41D8-BA13-DE93033EA108
Pensar el dispositivo escolar en diálogo con la obra de Boaventura de Sousa Santos
Authors: Vercellino Soledad 
Published: May 2022

Desde la década del 90 la investigación educativa ha puesto el foco en aquellos componentes estructurantes y estructurales de lo escolar, es decir, aquellos elementos invariantes que organizan la experiencia escolar. La ‘caja negra de lo escolar’ ha sido conceptualizada, segúnla tradición conceptual de la que se trate, como ‘gramática’ (Tyack y Cuban, 1995), ‘cultura’ (Julia, 2001; Viñao, 2002), ‘forma’ (Vincent, Lahire y Thin, 1994) o ‘dispositivo escolar’ (Querrien, 1979; Varela y Álvarez Uría, 1991). En esta comunicación se analizará la noción focoultiana de dispositivo y se intentará enriquecerla al ponerla en diálogo con la obra del Prof. Boaventura de Sousa Santos. Todo ellocomo forma de desarrollar un enfoque conceptual que permita pensar de otro modo lo escolar. Se trata de una estrategia de pensamiento, que otea ese espacio abrumador, enérgico, a veces inefable, otras trivializado en su aparente obviedad, que es ‘lo escolar’, intentando restituir algo de su multiplicidad y complejidad. Think the school dispositive into dialogue with the work of Boaventura de Sousa Santos
A experiência e a formação jurídica sob a perspectiva de promoção da justiça cognitiva
Authors: Palmeira Sobrinho Zéu 
Published: May 2022

Tem-se como objetivo discutir sobre a formação jurídica por mediação deexperiência no contexto da prática da justiça cognitiva. Propõe-se fazer uma análise sobre os principais problemas que o profissional do Direito possuem no processo de sua formação a partir da perspectiva de uma educação jurídica emancipatória, focada principalmente no caso de formação de juízes.
Integrando el papel de la agencia en los estudios sobre género y gabinetes ministeriales
Authors: Martelotte Lúcia 
Published: May 2022

Aunque con un grado de desarrollo inferior al alcanzado por los análisis sobregénero y Poder Legislativo, en las últimas décadas se han multiplicado los esfuerzos por indagar en el papel de las mujeres en el Poder Ejecutivo. Las investigaciones centradas en las mujeres en los gabinetes responden generalmente a diseños cuantitativos, en los que el énfasis está puesto en identificar el tipo de factores que obstaculizan la llegada de las mujeresa las posiciones ministeriales, así como el peso relativo de cada uno de estosfactores. Sin embargo, pasados casi veinte años desde que comenzaron a explorarse estos fenómenos, poco se sabe aún acerca de la capacidad de agencia de las mujeres que efectivamente lograron sortear dichos obstáculos y acceder a posiciones ministeriales. En la primera parte del trabajo se ofrece una sistematización de la literatura sobre género y poder ejecutivo en general y sobre mujeres en los gabinetes en particular. En la segunda parte se presentan argumentos que ponen de manifiesto la necesidad de incorporar la capacidad de agencia de las mujeres, junto con propuestas acerca de cómo definir e introducir este concepto y sus consecuencias en los análisis sobre género y gabinetes.
EDUCAÇÃO E EMANCIPAÇÃO: a perspectiva da Lei Federal 10.639/2003
Authors: Paula Benjamin Xavier 
Published: May 2022

Aborda-se a implementação da Lei Federal 10.639 de 09 de janeiro de 2003, bem como, seu arcabouço jurídico normativo, o Parecer CNE/CP nº 3/2004 e a Resolução CNE/CP nº 1/2004 seus significados e intenções, numa perspectiva teórico-metodológica que buscaarticular uma educação antirracista com os pressupostos éticos e estéticos de uma educação para a emancipação social, amparado na perspectiva dos conceitos de Boaventura de Sousa Santos (2010),Paulo Freire (2007) e Ana Maria Saul (1988), bem com, nos referenciais radicadas na experiência africana das diásporas, como disposto em Joseph Ki-Zerbo (2010), Elisa Larkin Nascimento (1996; 2008; 2009), Cheikh Anta Diop (1974, apud NASCIMENTO, 1996); KabengeleMunanga (1986; 2000; 2003; 2006), Neuza Santos Souza (1983), Frantz Fanon (2008), Abdias do Nascimento (2002), Carlos More(2007), Molefi Kete Asante (1987; 1989;1990), Jacques D’Adesk (2001), Dagoberto José Fonseca (2008), Luiz Alberto Oliveira Gonçalves; Petronilha Beatriz Gonçalves e Silva (2000), e os dados empíricos dapesquisa levantados a partir da pesquisa bibliográfica e pesquisa documental.
SEXUALIDADE E PARTO: em busca do elo perdido
Authors: Luz Lia 
Published: May 2022

Tendo-se como base metodológica a sociologia das ausências e a sociologia das emergências proposta por Boaventura de Sousa Santos, este artigo realiza uma cartografia simbólica teórica e práticasobre a relação entre sexualidade e parto. Na parte inicial, debate-se como o paradigma hegemônico da biomedicina, o chamado modelo tecnocrático, conforme categorização proposta por Davis-Floyd,foi dissociando o parto da sua natureza sexual. Em seguida, aborda-se a sexualidade do parto a partir de uma revisão da literatura que incluiu o resultado de pesquisas acadêmicas mais recentes sobre a temática, criando uma base teórica que situa o parto enquanto evento inerentemente sexual. No campo empírico, a temática é abordada a partir das narrativas e da percepção pessoal de mãesda comunidade intencional de Tamera, no sul de Portugal, escolhida como lócus de pesquisa por manter uma cultura de (amor e) sexualidade livre e também por incentivar os partos domiciliares planejados,dois ingredientes fundamentais para permitir a religação do elo entre sexualidade e parto. Tal etapa foi conduzida com o propósito de identificar como as mulheres vivenciam ou expressam sua sexualidadeno trabalho de parto e parto. Conclui-se que o parto é um evento intrinsecamente sexual, bastante marcante no ciclo sexual das mulheres, além de ser considerado extremamente empoderador, com consequências não apenas para a vida sexual, mas para a vida delas como um todo.
ESTADO Y PARENTESCO COMO FORMAS DE ARTICULACIÓN SOCIAL EN EL ANTIGUO EGIPTO: nuevas investigaciones históricoantropológicas sobre dinámicas políticas, económicas e ideológicas
Authors: Hernan Zapata Horacio Miguel 
Published: May 2022

CAMPAGNO, Marcelo (Ed.). Estudios sobre parentesco y Estado en el Antiguo Egipto. Buenos Aires: IHAO-FFyL-UBA/Ediciones Del Signo, 2006. 228 páginas.
Age Related Senescence, Apoptosis, and Inflammation Profiles in
Periodontal Ligament Cells from Canine Teeth
Authors: Zayed Mohammed , Iohara Koichiro 
Published: May 2022

Objective:
The periapical tissues, including periodontal ligament cells
(PDLCs) play an important role in repairing the surrounding tissue of the teeth. A
decrease in the regenerative potentiality of resident stem cells (PDLCs) has been
suggested to be attributed to the decline of pulp regeneration. Therefore, examining the
functional changes in periodontal tissue and cells that occur during the aging process is
necessary.

Methods:
The changes in the cementum extract (CE) and PDLCs isolated from young
and aged dog teeth were evaluated. PDLCs growth rate, senescence markers, p16 and
p21, and proinflammatory cytokines, IL-6, IL-1β, and TNF-α, were analyzed by RT-PCR.
Bax, an apoptosis marker, Bcl-2, a marker for cell survival, and IL-6 were examined by
Western blot analyses to detect their variance expression in the CE.

Results:
Our results demonstrated that aged PDLCs exhibit a low growth rate and an
increased expression of p16; however, no change has been demonstrated in the
expression of p21. The chronic inflammatory molecules, IL-6 and TNF-α, were
significantly upregulated compared to young PDLCs. Western blot analyses showed
decreased expression of Bcl-2 in the CE of the aged tooth (p < 0.001).

Conclusion:
Taken together, aging influences the functional changes of PDLCs and CE
and increases senescence, chronic inflammation, and apoptosis markers. As a result,
donor age is a key factor influencing the utilization of PDLCs for tooth regeneration.