bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous reconstruction of septic composite defects in lower extremities: Combination of fasciocutaneous perforator flap and Masquelet technique
Authors: Liu Xuanzhe , Yang Jin , Wang Hongshu , Lu Shengdi , Fan Cunyi , Wen Gen 
Published: August 2022

BackgroundManagement of composite defects with deep infection is a challenge to reconstructive surgeons. This study aimed to demonstrate the versatility, safety, and complications of simultaneous reconstruction of infectious composite defects with fasciocutaneous perforator flap combined with the Masquelet technique.MethodsThis study presents 10 patients in whom a fasciocutaneous perforator flap combined with the Masquelet technique was used to restore soft tissue and bone defects of the lower extremity, and were admitted in two level 1 trauma centers in Shanghai. The first stage included debridement of necrotic bone and infected tissues, implantation of a polymethylmethacrylate cement spacer to cover the void; bridging fixation of the osseous defect using external or internal fixators, and soft-tissue reconstruction with a fasciocutaneous perforator flap. The second stage included cement spacer removal with membrane preservation, refreshing bone edges, and grafting the cavity with bone morphogenetic proteins and autologous iliac bone graft.ResultsThe mean follow-up duration after autologous bone graft was 17.5 months. The average bony defects and average flap dimensions were 7.1 cm and 44.9 cm2, respectively. All flaps survived uneventfully. No recurrence of infection was detected in either the second stage of surgery or follow-up period. The mean duration of bone consolidation was 31.9 weeks. One patient had a 2 cm leg length discrepancy, and one patient had mild foot drop. No residual deformity requiring a secondary procedure occurred.ConclusionFasciocutaneous perforator flap combined with Masquelet technique provides a reliable and versatile alternative for patients with composite defects resulting from lower extremity infection.
Weighted gene co-expression identification of CDKN1A as a hub inflammation gene following cardiopulmonary bypass in children with congenital heart disease
Authors: Chen Huan , Liu Jinglan , Wu Yuqing , Jiang Li , Tang Mi , Wang Xin , Fang Xiaoling , Wang Xi 
Published: August 2022

BackgroundCongenital heart disease (CHD) is the most common type of birth defect. Most patients with CHD require surgery, and cardiopulmonary bypass (CPB) is the most common surgery performed.MethodsThe present study utilized weighted gene co-expression network analysis (WGCNA) to identify key inflammation genes after CPB for CHD. The GSE132176 dataset was downloaded from the Gene Expression Omnibus(GEO) database for WGCNA to identify the modules closely related to clinical traits. Disease enrichment, functional annotation and pathway enrichment were performed on genes in the module closely related to clinical traits using Enrichr and Metascape. Immune infiltration analysis was also performed on the training dataset using CIBERSORT. Finally, we identified hub genes using high gene significance (GS), high module members (MMs) and Cytoscape, and we verified the hub genes using an independent dataset and Western blot analysis.ResultsWGCNA showed that the brown module with 461 genes had the highest correlation to CHD after CPB. Functional annotation and pathway enrichment analysis were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, which showed that genes in the brown module were enriched in inflammation-related pathways. In the disease enrichment analysis, genes in the brown module were enriched for inflammatory diseases. After the 30 most highly associated brown intramodular genes were screened, a protein-protein interaction network was constructed using the STRING online analysis website. The protein-protein interaction results were then calculated using 12 algorithms in the cytoHubba plugin of Cytoscape software. The final result showed that CDKN1A was the fundamental gene of post-CPB for CHD. Using another independent validation dataset (GSE12486), we confirmed that CDKN1A was significantly differentially expressed between preoperative and postoperative CPB (Wilcoxon, P = 0.0079; T-test, P = 0.006). In addition, CDKN1A expression was elevated in eosinophils, neutrophils, memory CD4 T cells and activated mast cells. Western blot analysis showed that the expression of CDKN1A protein was significantly higher postoperative CPB than preoperative CPB. Moreover, CDKN1A was mainly related to inflammation.ConclusionIn summary, we found a relationship between CDKN1A and inflammation after CPB for congenital heart disease by WGCNA, experiments and various bioinformatics methods. Thus, CDKN1A maybe serve as a biomarker or therapeutic target for accurate diagnosis and treatment of inflammation after CPB in the future.
Gene Expression Patterns of Osteopontin Isoforms and Integrins in Malignant Melanoma
Authors: Jámbor Krisztina , Koroknai Viktória , Kiss Tímea , Szász István , Pikó Péter , Balázs Margit 
Published: August 2022

Osteopontin (OPN) is a multifunctional glycoprotein that physiologically interacts with different types of integrins. It is considered to be a possible prognostic biomarker in certain tumor types; however, various splicing isoforms exist, which have not been investigated in melanoma. We aimed to define the relative expression pattern of five OPN isoforms and clarify the prognostic significance of the splice variants in melanoma. We also aimed to investigate the expression pattern of eight integrins in the same tumors. Gene expression analyses revealed that the relative expression of OPNa, OPNb, and OPNc is significantly higher in metastatic tumors compared to primary lesions (p &lt; 0.01), whereas the expression of OPN4 and OPN5 was low in both. The more aggressive nodular melanomas had higher expression levels compared to the superficial spreading subtype (p ≤ 0.05). The relative expression of the eight tested integrins was low, with only the expression of ITGB3 being detectable in nodular melanoma (Medianlog2 = 1.274). A positive correlation was found between Breslow thickness and the expression of OPNc variant, whereby thicker tumors (&gt;4 mm) had significantly higher expression (p ≤ 0.05). The Breslow thickness was negatively correlated with the expression of OPN4, and similarly with ITGA2. OPNc also exhibited significant positive correlation with the presence of metastasis. Our data show that high expression of OPNa, OPNb, and especially OPNc and low expression of OPN4 and ITGA2 are associated with an advanced stage of tumor progression and poor prognosis in melanoma.
New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species Sanghuangporusbaumii (Pilát) L.W. Zhou & Y.C. Dai
Authors: Liu Zengcai , Liu Ruipeng , Tong Xinyu , Zou Li 
Published: August 2022

Triterpenoids are secondary metabolites produced by the fungus Sanghuangporus baumii that have important pharmacological activities. However, the yield of triterpenoids is low and cannot meet market demand. Here, we treated S. baumii with several concentrations of MeJA (methyl jasmonate) and found that the total triterpenoid content was highest (23.31 mg/g) when the MeJA concentration was 250 μmol/L. qRT-PCR was used to quantify the transcription of five key genes involved in triterpenoid biosynthesis. The results showed that the relative transcription of most genes increased with increasing MeJA concentration, indicating that MeJA is a potent inducer of triterpenoid biosynthesis in S. baumii. To further explore whether other terpenoid biosynthesis pathways are also involved in the accumulation of triterpenoids induced by MeJA, we measured the contents of cis-Zeatin (cZ), gibberellins (GAs), and the transcript levels of related biosynthesis genes. We found that MeJA significantly inhibited the biosynthesis of cZ, GAs, and the transcription of related genes. The repressive effects of MeJA on cZ and GA accumulation were further confirmed by growth rate and biomass assays. In conclusion, our study provides an effective method to enhance the triterpenoid content of S. baumii, and also provides novel insights into the mechanism of MeJA-induced triterpenoid biosynthesis.
Technological Unemployment in the Perspective of Industry 4.0
Authors: Kuzior Aleksandra 
Published: August 2022

The article concerns the problem of technological unemployment in the perspective of industry 4.0 development. The purpose of the article is to indicate the positive and negative effects of industry 4.0 development and to define the ways of programming education as a way to counteract the negative effects of industry 4.0 development. The author emphasizes the need for an appropriate education curriculum, aimed at acquiring both professional and engineering competences as well as humanistic, ethical and social competences at the same time. Technological unemployment is defined as a temporary, short-term phenomenon, lasting until human capital is shifted to other applications. The lack of adequate education, including lifelong learning, creates a risk of transition from technological unemployment to structural unemployment, which is much more dangerous for economic and social reasons. The article, therefore, contains an analysis of the labor market in terms of the demand for specific competences in the perspective of industry 4.0 development and forecasts of the demand for competences of the future. There is no doubt that the development of industry 4.0 requires specialized competences combining the skills of an IT specialist and an automation / robotics specialist who has skills in the field of cyber-physical systems integration, operation of advanced production management systems and complex production data analysis systems as well as the application of artificial intelligence algorithms in the production space. At the same time, however, specialists with high communication, interpersonal and social competences will be sought, hence there is a need for appropriate education curriculum.
Arquitetura francesa em dois tempos
Authors: Fiori Arantes Otilia Beatriz 
Published: August 2022

Pouco mais de 25 anos separam os dois ensaios que compõem este volume. O primeiro foi redigido e publicado em 1988. O segundo, embora escrito em 2014, permanecia inédito. Reunidos agora na intenção de um balanço, pretendem sobretudo dar conta das transformações da arquitetura na França através de dois momentos, embora de grande animação, extremamente diferentes. Ou seja, que vão do rescaldo de 1968, do qual o Beaubourg (1976) é a expressão mais acabada, quando o Estado elege a cultura como política oficial — os grandes (e vistosos) equipamentos culturais (em especial os Grandes Projetos da Era Mitterand), acompanhados de seu contraponto complementar de valorização patrimonial e de uma arquitetura “modesta” e contextual—, a um novo surto no qual a França, e sobretudo Paris e seu entorno, cedem à corrida global pelos ativos imobiliários, de alto bordo, disputados pelas construtoras e os escritórios de ponta, que passaram, ao menos desde a virada de século, a dar as cartas (mesmo quando subsidiadas pelo Estado). Momento este em que a convergência entre cultura e mundo dos negócios (já presente obviamente na primeira fase — “a cultura é o nosso petróleo”, dizia o Ministro Jacques Lang) cede de vez às estritas imposições desse último e, neste vale tudo, novas frentes vão obrigatoriamente se abrindo, os inúmeros centros culturais sendo substituídos paulatinamente por butiques (mesmo quando butiques museus), prédios corporativos, edifícios públicos (ou que por natureza deveriam sê-lo), vinícolas, shoppings ou equipamentos esportivos. Nestes novos projetos, como dizia com pertinência François Chaslin, “as equipes são impressionantes; os aspectos financeiros, fundamentais, e as questões arquitetônicas relativamente secundárias”. Onda global, sem dúvida, mas em que a França, de sua posição de inegável vanguarda, ao menos em relação à grande virada cultural do último quarto do século XX, passa à de mera caudatária. Este duplo balanço, o primeiro elaborado às vésperas do bicentenário da Revolução Francesa e no auge do mandato de Mitterand, o segundo, ao se encerrar o ciclo conservador com Sarkozy na presidência, serve também de pretexto para ilustrar os caminhos e descaminhos da arquitetura mundial em meio século: dos grandes ideais (e projetos) aos grandes negócios — quando ela passa a obedecer antes de tudo aos imperativos do mercado, patinando nos excessos da irrelevância estética (apesar das extravagâncias formais). O percurso desses textos vai assim do Beaubourg à Fundação Vuitton e suas variantes mais prosaicas.

Palavras-chave: Animação cultural, Arquitetura modesta, Beaubourg, Bernard Huet, François Chesnais, Gaudin, Gehry, Grand Louvre, Grandes Projetos, Grumbach, Jacques Lang, Jean Nouvel, La Défense, Mitterrand, Movimento Moderno, Patrimônio, Pei, Perrault, Portzamparc, Renzo Piano, Ricciotti, Rogers, Sarkozy
Avaliação de métodos para estimar a infestação da Traça-da-castanha em estudo sobre a resposta de clones de cajueiro ao ataque da praga
Authors: Mesquita Antonio Lindemberg Martins , Lima Raimundo Nonato de , Teixeira Pâmela Brenna Silva , Soares João Victor de Souza , Carvalho Yago Lourenço de 
Published: August 2022

A traça-da-castanha (Anacampsis phytomiella Busck, Lepidoptera: Gelechiidae) é considerada a principal praga dos frutos do cajueiro devido aos elevados prejuízos que causa por destruir completamente a amêndoa da castanha-de-caju. Este trabalho teve como objetivos: 1) comparar a eficiência de dois métodos de amostragens do ataque da traça-da-castanha; 2) avaliar o grau de infestação da praga em duas épocas do período produtivo de clones de cajueiro-anão. O trabalho foi realizado no segundo semestre de 2015 na Estação Experimental da Embrapa Agroindústria Tropical em Pacajus, CE. Para avaliações dos graus de infestação da traça-da-castanha na planta e no solo, foram adotados dois procedimentos de amostragem. No primeiro, o grau de infestação foi calculado amostrando-se cinco castanhas no estágio de máximo desenvolvimento (castanhas verdes na planta), em cada quadrante, totalizando 20 castanhas por planta. No segundo, o grau de infestação foi determinado por meio de uma amostra de 200 castanhas coletadas no solo, debaixo das copas dos cajueiros. Para os dois métodos, as avaliações foram feitas em 20 plantas dos cinco clones CCP 1001, CCP 76, CCP 09, BRS189 e CCP 06, e o grau de infestação foi dado pelo percentual de castanhas de caju com perfurações, as quais são normalmente observadas na ponta da castanha. Assumindo-se que o cálculo do percentual de castanhas furadas colhidas no solo (debaixo da copa) representa a perda econômica real causada pela praga e considerando que esse valor não difere estatisticamente do obtido por meio da avaliação amostral na planta, conclui-se a partir dos dados obtidos que este último procedimento é suficientemente preciso na determinação do grau de infestação ocasionado pela traça-da-castanha e que, pela rapidez e facilidade na execução, esse método pode ser utilizado para dar suporte a decisões de controle dentro das premissas do manejo integrado de pragas. O clone CCP 1001, seguido do clone CCP 76, foi o genótipo que apresentou o maior grau de infestação, em que mais da metade das castanhas colhidas tiveram suas amêndoas destruídas pela praga. O percentual de castanhas furadas apresentou uma tendência de redução ao longo do ciclo produtivo do cajueiro; ou seja, o grau da infestação decresce do início para o fim da safra
Pelanggaran kapal perikanan di wilayah pengelolaan perikanan negara Republik Indonesia 571
Authors: Marpaung Bella S.M. , Sitanggang Effendi P , Masengi Kawilarang W.A. , Kaparang Frangky E. , Pamikiran Revols D.Ch. , Sitanggang Dyan F.D. , Kalalo Flora F. 
Published: August 2022

Wilayah Pengelolaan Perikanan Negara Republik Indonesia WPP-571 sesuai Permen KP mencakup perairan Laut Andaman dan Selat Malaka. Perairan Selat Malaka merupakan perairan yang berbatasan langsung dengan batas wilayah maritim negara tetangga yaitu Malaysia. Perairan ini sebagai wilayah perairan yang cukup potensial sangat memungkinkan terjadinya praktek penangkapan ikan secara tidak sah. Dalam hal kegiatan pengawasan kapal perikanan yang ada WPP- 571, kepatuhan nelayan terhadap kegiatan aktivitas penangkapan ikan masih belum efektif atau masih belum maksimal dan ditambah lagi meningkatnya jumlah kapal perikanan yang beroperasi di WPP- 571. Informasi tentang pelanggaran kapal penangkapan ikan perlu di deskripsikan agar dapat menjadi informasi yang berguna untuk mengevaluasi kegiatan pengawasan kapal perikanan di WPP- 571.Tujuan dari penelitian ini adalah untuk mengetahui kesesuaian antara daerah operasi penangkapan ikan dengan izin operasi penangkapan ikan di WPP.menganalisis jenis pelanggaran yang terpantau di WPP-571 dan dan mengetahui partisipasi nelayan di PPS Belawan dalam mengurangi illegal fishing. Penelitian ini akan diawali dengan pengajuan surat hingga pada pengerjaan laporan penelitian. Rencana pengambilan data penelitian ini bertempat di Stasiun PSDKP Belawan dan PPS Belawan di Provinsi Sumatera Utara.
ANALISIS KONTRIBUSI PAJAK KENDARAAN BERMOTOR (PKB) TERHADAP PENDAPATAN ASLI DAERAH (PAD) DI KOTA PALOPO
Authors: Wahida Wahida , Sorong Arjun 
Published: August 2022

Penelitian ini bertujuan untuk mengetahui kontribusi pajak kendaraan bermotor terhadap Pendapatan Asli Daerah (PAD) Kota Palopo sejak tahun 2012 sampai dengan tahun 2015. Jenis penelitian ini adalah penelitian kuantitatif dengan data sekunder yang diperoleh berdasarkan data Pajak Kendaraan Bermotor dan Pendapatan Asli Daerah Kota Palopo mulai tahun 2012-2015. Analisis data digunakan dalam penelitian ini adalah analisis kontribusi dalam menghitung kontribusi Pajak Kendaraan Bermotor (PKB) terhadap Pendapatan Asli Daerah (PAD) periode 2012-2015. Hasil penelitian yang diperoleh menunjukkan bahwa kontribusi Pajak Kendaraan Bermotor (PKB) terhadap Pendapatan Asli Daerah (PAD) di Kota Palopo periode 2012-2015 adalah sangat baik, dengan nilai rata-rata adalah sebesar 55,20% dan Pajak Kendaraan Bermotor (PKB) jenis motor memberikan kontribusi yang baik terhadap Pendapatan Asli Daerah (PAD) di Kota Palopo dengan nilai rata-rata sebesar 43,15% jika dibandingkan dengan bus yang nilai rata-ratanya hanya sebesar 0,81% dan mobil barang rata-rata sebesar 10,83%.
PROSES PERANCANGAN MESIN PENEPUNG BIJI-BIJIAN DENGAN METODE VDI 2221
Authors: , Sumiyanto , Saputra Rudi 
Published: August 2022

ABSTRACT

The purpose of making a grain cutting machine is to process grains into flour so that it is easy to process in various types of food. This grain cutting machine can also be used as a business field for small and medium micro. The design of this grain cutting machine uses the VDI 2221 method. Using the VDI 2221 method, there are three variations of the choice of grain cutting machines. Of the three variations of the choice of the seed-making machine , the grain is selected the third choice variation because it suits the list of the will and desire of the designer. The grain cutting machine retaining engine uses gasoline motor propulsion using a V belt transmission system. This type of crusher uses a hammer mill as a grain crusher. This grain cutting machine has dimensions of 50 x 80 x 100 cm and has wheels equipped to facilitate mobility so that it can be moved easily. The use and maintenance of the machine is easy to do. Manufacturing costs are cheap and materials are easy to obtain.

Keywords : The Grains Cutting Machine, Hammer Mill, VDI Method 2221. Vbelt
ON THE ISSUE OF DIGITAL EDUCATIONAL RESOURCES
Authors: Попова Ксения Владимировна , Малышева Елена Николаевна 
Published: August 2022

В статье рассматриваются особенности цифровых образовательных ресурсов (ЦОР) и возможности их использования в учебном процессе в контексте актуальных задач школьного образования. Выделены виды современных ЦОР, продемонстрированы результаты их анализа в классификации по цели применения на разных этапах урока. Обозначены проблемы внедрения и использования ЦОР, связанные с готовностью педагогов создавать качественный информационный продукт учебного назначения, который соответствует всем дидактическим и эргономическим требованиям.
The article discusses the features of digital educational resources (DER) and the possibilities of their use in the educational process in the context of the actual tasks of school education. The types of modern DER are highlight the results of their analysis in the classification according to the purpose of application at different stages of the lesson are demonstrated. The problems of the introduction and use of the DER associated with the willingness of teachers to create a high-quality educational information product that meets all didactic and ergonomic requirements are outline.
THE PROBLEM OF FORMING THE PROJECT COMPETENCE OF FUTURE TEACHERS
Authors: Клушина Надежда Павловна , Салпагарова Мадина Бурхановна 
Published: August 2022

В статье обосновывается значимость проектной деятельности для подготовки современных школьников к эффективному социальному функционированию в современных условиях. Рассматривается сущность и структура проектной компетентности будущих педагогов. Выделяются условия ее формирования в процессе профессиональной подготовки педагогических кадров.
The article substantiates the importance of project activities for preparing modern schoolchildren for effective social functioning in modern conditions. The essence and structure of the project competence of future teachers is considered. The conditions for its formation in the process of professional training of teaching staff are highlighted.
DYNAMICS AND STRUCTURE OF RUSSIAN STATE BUDGET EXPENDITURES AT THE PRESENT STAGE OF DEVELOPMENT
Authors: Недорезова Елена Сергеевна , Благова Полина Игоревна 
Published: August 2022

Рассматриваются статистические данные о расходах государственного бюджета за 2018-2022 годы, выявляются принципы организации его расходной части, анализируется грамотность реализации затрат и в целом экономическая политика государства.
In this article statistical data on the expenditures of the state budget for 2018-2022 are considered, the principles of organizing its expenditure side are identified, the literacy of the implementation of costs and, in general, the economic policy of the state are analyzed.
COMMUNICATION PROJECT TO PROMOTE THE FASHION BRAND
Authors: Полунина Ольга Сергеевна , Муссаралиев Донат Амангалиевич 
Published: August 2022

Лишение малого и среднего бизнеса популярных и удобных площадок для продвижения в сети-интернет.
Deprivation of small and medium-sized businesses of popular and convenient platforms for promotion on the Internet.
TAX SYSTEM AND WAYS TO IMROVE IT
Authors: Киргизова Маргарита Сергеевна , Недорезова Елена Сергеевна 
Published: August 2022

Налоговая система - основа экономически сильного государства, поэтому важно обращать большое внимание на ее своевременное совершенствование, позволяющее обеспечивать достойный уровень жизни в данном государстве.
Tax system - the foundation of economically powerful government, that’s why it is important to take into account timely improving this system, enabling a decent standard of living in this government.