bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in interstitial fluid flow, mass transport and the bone cell response in microgravity and normogravity
Authors: Wei Fei , Flowerdew Kendal , Kinzel Michael , Perotti Luigi E. , Asiatico Jackson , Omer Mahmoud , Hovell Candice , Reumers Veerle , Coathup Melanie J. 
Published: November 2022

AbstractIn recent years, our scientific interest in spaceflight has grown exponentially and resulted in a thriving area of research, with hundreds of astronauts spending months of their time in space. A recent shift toward pursuing territories farther afield, aiming at near-Earth asteroids, the Moon, and Mars combined with the anticipated availability of commercial flights to space in the near future, warrants continued understanding of the human physiological processes and response mechanisms when in this extreme environment. Acute skeletal loss, more severe than any bone loss seen on Earth, has significant implications for deep space exploration, and it remains elusive as to why there is such a magnitude of difference between bone loss on Earth and loss in microgravity. The removal of gravity eliminates a critical primary mechano-stimulus, and when combined with exposure to both galactic and solar cosmic radiation, healthy human tissue function can be negatively affected. An additional effect found in microgravity, and one with limited insight, involves changes in dynamic fluid flow. Fluids provide the most fundamental way to transport chemical and biochemical elements within our bodies and apply an essential mechano-stimulus to cells. Furthermore, the cell cytoplasm is not a simple liquid, and fluid transport phenomena together with viscoelastic deformation of the cytoskeleton play key roles in cell function. In microgravity, flow behavior changes drastically, and the impact on cells within the porous system of bone and the influence of an expanding level of adiposity are not well understood. This review explores the role of interstitial fluid motion and solute transport in porous bone under two different conditions: normogravity and microgravity.
Adaptive insertion of a hydrophobic anchor into a poly(ethylene glycol) host for programmable surface functionalization
Authors: Zhang Shaohua , Li Wei , Luan Jiabin , Srivastava Abhinav , Carnevale Vincenzo , Klein Michael L. , Sun Jiawei , Wang Danni , Teora Serena P. , Rijpkema Sjoerd J. , Meeldijk Johannes D. , Wilson Daniela A. 
Published: November 2022

AbstractCovalent and non-covalent molecular binding are two strategies to tailor surface properties and functions. However, the lack of responsiveness and requirement for specific binding groups makes spatiotemporal control challenging. Here, we report the adaptive insertion of a hydrophobic anchor into a poly(ethylene glycol) (PEG) host as a non-covalent binding strategy for surface functionalization. By using polycyclic aromatic hydrocarbons as the hydrophobic anchor, hydrophilic charged and non-charged functional modules were spontaneously loaded onto PEG corona in 2 min without the assistance of any catalysts and binding groups. The thermodynamically favourable insertion of the hydrophobic anchor can be reversed by pulling the functional module, enabling programmable surface functionalization. We anticipate that the adaptive molecular recognition between the hydrophobic anchor and the PEG host will challenge the hydrophilic understanding of PEG and enhance the progress in nanomedicine, advanced materials and nanotechnology.
Quantum Hall phase in graphene engineered by interfacial charge coupling
Authors: Wang Yaning , Gao Xiang , Yang Kaining , Gu Pingfan , Lu Xin , Zhang Shihao , Gao Yuchen , Ren Naijie , Dong Baojuan , Jiang Yuhang , Watanabe Kenji , Taniguchi Takashi , Kang Jun , Lou Wenkai , Mao Jinhai , Liu Jianpeng , Ye Yu , Han Zheng , Chang Kai , Zhang Jing , Zhang Zhidong 
Published: November 2022

AbstractThe quantum Hall effect can be substantially affected by interfacial coupling between the host two-dimensional electron gases and the substrate, and has been predicted to give rise to exotic topological states. Yet the understanding of the underlying physics and the controllable engineering of this interaction remains challenging. Here we demonstrate the observation of an unusual quantum Hall effect, which differs markedly from that of the known picture, in graphene samples in contact with an antiferromagnetic insulator CrOCl equipped with dual gates. Two distinct quantum Hall phases are developed, with the Landau levels in monolayer graphene remaining intact at the conventional phase, but largely distorted for the interfacial-coupling phase. The latter quantum Hall phase is even present close to the absence of a magnetic field, with the consequential Landau quantization following a parabolic relation between the displacement field and the magnetic field. This characteristic prevails up to 100 K in a wide effective doping range from 0 to 1013 cm−2.
Topological morphogenesis of neuroepithelial organoids
Authors: Ishihara Keisuke , Mukherjee Arghyadip , Gromberg Elena , Brugués Jan , Tanaka Elly M. , Jülicher Frank 
Published: November 2022

AbstractAnimal organs exhibit complex topologies involving cavities and tubular networks, which underlie their form and function1–3. However, how topology emerges during the development of organ shape, or morphogenesis, remains elusive. Here we combine tissue reconstitution and quantitative microscopy to show that tissue topology and shape is governed by two distinct modes of topological transitions4,5. One mode involves the fusion of two separate epithelia and the other involves the fusion of two ends of the same epithelium. The morphological space is captured by a single control parameter that can be traced back to the relative rates of the two epithelial fusion modes. Finally, we identify a pharmacologically accessible pathway that regulates the frequency of two modes of epithelial fusion, and demonstrate the control of organoid topology and shape. The physical principles uncovered here provide fundamental insights into the self-organization of complex tissues6.
MesopTroph, a database of trophic parameters to study interactions in mesopelagic food webs
Authors: Silva Mónica A. , Fonseca Catarina T. , Olivar M. Pilar , Bernal Ainhoa , Spitz Jérôme , Chouvelon Tiphaine , Jonasdottir Sigrún , Colaço Ana , Carmo Vanda , Sutton Tracey , Menezes Gui , Falkenhaug Tone , Bergstad Odd Aksel , Pérez-Jorge Sergi 
Published: November 2022

AbstractMesopelagic organisms play a crucial role in marine food webs, channelling energy across the predator-prey network and connecting depth strata through their diel vertical migrations. The information available to assess mesopelagic feeding interactions and energy transfer has increased substantially in recent years, owing to the growing interest and research activity in the mesopelagic realm. However, such data have not been systematically collated and are difficult to access, hampering estimation of the contribution of mesopelagic organisms to marine ecosystems. Here we present MesopTroph, a georeferenced database of diet, trophic markers, and energy content of mesopelagic and other marine taxa compiled from 203 published and non-published sources. MesopTroph currently includes data on stomach contents, carbon and nitrogen stable isotopes, major and trace elements, energy density, fatty acids, trophic positions, and diet proportion estimates for 498 species/genera. MesopTroph will be expanded with new data emerging from ongoing studies. MesopTroph provides a unique tool to investigate trophic interactions and energy flow mediated by mesopelagic organisms, and to evaluate the ecosystem services of this community.
An epidemic context elicits more prosocial decision-making in an intergroup social dilemma
Authors: Rychlowska Magdalena , van der Schalk Job , Manstead Antony S. R. 
Published: November 2022

AbstractSocietal challenges such as the COVID-19 pandemic have the quality of a social dilemma, in that they compel people to choose between acting in their own interests or the interests of a larger collective. Empirical evidence shows that the choices people make in a social dilemma are influenced by how this decision is framed. In four studies, we examined how context of an epidemic influences resource allocation decisions in a nested social dilemma task, where participants share resources between themselves, their subgroup, and a larger collective. Participants consistently allocated more resources to the collective in the context of the Ebola epidemic than in the context of a neighborhood improvement project, and these choices were strongly associated with prescriptive social norms. Together, the findings provide an experimental demonstration that the context of a quickly spreading disease encourages people to act more prosocially.
Quantifying F-actin patches in single melanoma cells using total-internal reflection fluorescence microscopy
Authors: Sheykhi Elham , Shojaedin-Givi Behnaz , Sajad Batool , Naderi-Manesh Hossein , Tavaddod Sharareh 
Published: November 2022

AbstractTotal-internal reflection fluorescence (TIRF) microscope is a unique technique for selective excitation of only those fluorophore molecules in a cellular environment, which are located at the sub-diffraction axial distance of a cell’s contact-area. Despite this prominent feature of the TIRF microscope, making quantitative use of this technique has been a challenge, since the excitation intensity strongly depends on the axial position of a fluorophore molecule. Here, we present an easy-implemented data analysis method to quantitatively characterize the fluorescent signal, without considering the intensity-value. We use F-actin patches in single-melanoma cells as an example and define two quantities of elongation and surface density for F-actin patches at the contact-area of a melanoma cell. The elongation parameter can evaluate the dispersion of F-actin patches at the contact-area of a cell and is useful to classify the attaching, spreading, and expanding stages of a cell. Following that, we present the profile of the surface density of F-actin patches as a quantity to probe the spatio-temporal distribution of the F-actin patches at the contact-area of a cell. The data analysis methods that are proposed here will also be applicable in the image analysis of the other advanced optical microscopic methods.
Micro lesion effect of pallidal deep‑brain stimulation for meige syndrome
Authors: Liu Jiayu , Ding Hu , Xu Ke , Wang Dongliang , Ouyang Jia , Liu Zhi , Liu Ruen 
Published: November 2022

AbstractTo analyse the microlesion effect (MLE) in the globus pallidus interna (GPi) of deep brain stimulation (DBS) in patients with Meige syndrome. Thirty-two patients with primary Meige syndrome who underwent GPi-DBS in this study. Burke–Fahn–Marsden Dystonia Rating Scale scores (BFMDRS-M) were obtained for the evaluation of clinical symptoms at 3 days before DBS (baseline), 24 h after DBS surgery, once weekly for 1 month until electrical stimulation, 6 months postoperatively and 12 months after surgery. Twenty-seven patients had MLE after GPi-DBS. The mean time of BFMDRS-M scores maximal improvement from MLE was 35.9 h postoperatively (range, 24–48 h), and the mean scores improved by 49.35 ± 18.16%. At 12 months after surgery, the mean BFMDRS-M scores improved by 50.28 ± 29.70%. There was a positive correlation between the magnitude of MLE and the motor score at 12 months after GPi-DBS (R2 = 0.335, p < 0.05). However, there was no correlation between the duration of MLE and DBS improvement. Most Meige syndrome patients who underwent GPi-DBS and had MLE benefited from MLE. For Meige syndrome, MLE might be a predictive factor for patient clinical symptom improvement from DBS.
Elastomeric seal stress analysis using photoelastic experimental hybrid method
Authors: Mose Bruno R. , Shin Dong-Kil , Alunda Bernard O. , Nam Jeong Hwan 
Published: November 2022

AbstractStress freezing is an important and powerful procedure in 3-dimensional experimental stress analysis using photoelasticity. The application of the stress freezing technique to extract stress components from loaded engineering structures has, however, declined over the years even though its principles are well established. This is attributed to huge costs arising from energy consumption during the process. In addition, significant time is needed to generate the desired information from isoclinic and isochromatic fringes. To overcome the limitations of stress freezing in photoelasticity and transform it into an economical device for stress analysis in an engineering environment, a new stress freezing cycle that lasts 5 h is proposed. The proposed technique is used in several applications of elastomeric seals with different cross-sectional profiles to assess their suitability. It was found that reducing the cycle time can lead to huge energy savings without compromising the quality of the fringes. Moreover, the use of isochromatic only to extract stress components leads to a shorter processing time to achieve desirable information since the process of obtaining isoclinic data is involving. In this paper, results of stress analysis from stress-frozen elastomeric seals with various cross-sections using the new stress freezing cycle are presented.
Complications associated to wound drainages in tumor spine surgery: a multicenter surveillance study from the German Spine Registry (DWG-Register)
Authors: Walter Sebastian G. , Lenz Maximilian , Gaisendrees Christopher , Schlachtenberger Georg , Sircar Krishnan , Knöll Peter ,   DWG Registry Study Group, Siewe Jan , Brenke Christopher , Rommelspacher Yorck , Shiban Ehab , Bayerl Simon , Mehren Christoph , Vinas-Rios Juan Manuel , Zarghooni Kourosh 
Published: November 2022

AbstractThere is an ongoing debate whether a surgical drainage is beneficial to prevent local accumulation of hematoma and to reduce the rate of wound infections, and neurological deficits. Data from the German Spine Society (DWG) registry were filtered for surgically treated spine tumor cases between 2017 and 2021. Cases were categorized into with (Group I) and without (Group II) placement of a surgical drainage. Subgroups were compared for demographic data, type of surgery, experience of the surgeon and postoperative surgical complications. 10,029 cases were included into final analysis (Group I: 3007; Group II: 7022). There was no significant difference between both groups regarding age or gender distribution. Average morbidity of patients was significantly elevated in Group I (p < 0.05) and the rates of invasive surgery were significantly increased in this group (p < 0.001). Overall complication rates were reported with 12.0% (Group I) and 8.5% (Group II). There were significantly more epidural hematoma (p < 0.001) and motor dysfunction (p = 0.049) as well as deep wound infections (p < 0.001) and implant failures (p = 0.02) in Group I. A surgical wound drainage cannot prevent epidural hematoma.
Establishment of a digital PCR method for detection of Borrelia burgdorferi sensu lato complex DNA in cerebrospinal fluid
Authors: Leth Trine Andreasen , Joensen Sara Moeslund , Bek-Thomsen Malene , Møller Jens Kjølseth 
Published: November 2022

AbstractDirect detection of Borrelia burgdorferi sensu lato bacteria in patient samples for diagnosis of Lyme neuroborreliosis (LNB) is hampered by low diagnostic sensitivity, due to few bacteria in cerebrospinal fluids (CSF) samples. Evaluation of novel molecular methods, including digital PCR (dPCR), as future tools in diagnostics of LNB is desirable. This study aimed to establish a dPCR assay and validate pre-PCR procedures for detection of Borrelia in CSF. Synthetic DNA fragments and cultured Borrelia reference strains were used during optimisation experiments. In addition, 59 CSF specimens from patients examined for LNB were included for clinical validation. The results showed that the pre-PCR parameters with the highest impact on Borrelia-specific dPCR method performance were incubation of the PCR-plate at 4 °C for stabilization of droplets, centrifugation for target concentration, quick-spin for dPCR rain reduction, and PCR inhibition by matrix components. Borrelia DNA in CSF was detected in one out of nine patients with LNB. Diagnostic sensitivity was determined to be 11.1% and specificity 100%. In conclusion, this study reports an optimized Borrelia-specific dPCR method for direct detection of Borrelia in CSF samples. The present study does not support the use of Borrelia-specific dPCR as a routine method for diagnosing LNB.
An odorant-binding protein in the elephant's trunk is finely tuned to sex pheromone (Z)-7-dodecenyl acetate
Authors: Zaremska Valeriia , Renzone Giovanni , Arena Simona , Ciaravolo Valentina , Buberl Andreas , Balfanz Folko , Scaloni Andrea , Knoll Wolfgang , Pelosi Paolo 
Published: November 2022

AbstractChemical communication in elephants has been well studied at the chemical and behavioural levels. Pheromones have been identified in the Asian elephant (Elephas maximus), including (Z)-7-dodecenyl acetate and frontalin, and their specific effects on the sexual behaviour of elephants have been accurately documented. In contrast, our knowledge on the proteins mediating detection of pheromones in elephants remains poor and superficial, with only three annotated and reliable entries in sequence databases, two of them being odorant-binding proteins (OBPs), and the third a member of von Ebner's gland (VEG) proteins. Proteomic analysis of trunk wash extract from African elephant (Loxodonta africana) identified one of the OBPs (LafrOBP1) as the main component. We therefore expressed LafrOBP1 and its Asian elephant orthologue in yeast Pichia pastoris and found that both recombinant proteins, as well as the natural LafrOBP1 are tuned to (Z)-7-dodecenyl acetate, but have no affinity for frontalin. Both the natural and recombinant LafrOBP1 carry post-translational modifications such as O-glycosylation, phosphorylation and acetylation, but as these modifications affect only a very small amount of the protein, we cannot establish their potential effects on the ligand-binding properties of OBP1.
Conformational deformation of a multi-jointed elastic loop
Authors: Tanaka Hiro , Seki Yuji , Ueno Shohei , Shibutani Yoji 
Published: November 2022

AbstractA new class of deformation is presented for a planar loop structure made up of slender elastic bodies and joints. In demonstrating the circumferential shortening of the multi-jointed elastic loop, diverse three-dimensional (3D) deformations emerge through piecewise deflections and discrete rotations. These 3D morphologies correspond to conformations of molecular ring systems. Through image processing, the 3D reconstructions of the deformed structures are characterized by number, geometry, and initial imperfections of the body segments. We elucidate from measurements that the conformational deformation without self-stress results from a cyclical assembly of compressive bending of elastic bodies with high shear rigidity. The mechanical insights gained may apply in controlling the polymorphism exhibited by the cyclical structures across scales.
Population admixtures in medaka inferred by multiple arbitrary amplicon sequencing
Authors: Fujimoto Shingo , Yaguchi Hajime , Myosho Taijun , Aoyama Hiroaki , Sato Yukuto , Kimura Ryosuke 
Published: November 2022

AbstractCost-effective genotyping can be achieved by sequencing PCR amplicons. Short 3–10 base primers can arbitrarily amplify thousands of loci using only a few primers. To improve the sequencing efficiency of the multiple arbitrary amplicon sequencing (MAAS) approach, we designed new primers and examined their efficiency in sequencing and genotyping. To demonstrate the effectiveness of our method, we applied it to examining the population structure of the small freshwater fish, medaka (Oryzias latipes). We obtained 2987 informative SNVs with no missing genotype calls for 67 individuals from 15 wild populations and three artificial strains. The estimated phylogenic and population genetic structures of the wild populations were consistent with previous studies, corroborating the accuracy of our genotyping method. We also attempted to reconstruct the genetic backgrounds of a commercial orange mutant strain, Himedaka, which has caused a genetic disturbance in wild populations. Our admixture analysis focusing on Himedaka showed that at least two wild populations had genetically been contributed to the nuclear genome of this mutant strain. Our genotyping methods and results will be useful in quantitative assessments of genetic disturbance by this commercially available strain.
Theoretical study on thermal decomposition mechanism of 1-nitroso-2-naphthol
Authors: Fan Xiaohua , Gan Yixiu , Tan Miaowen , Wang Wenhe 
Published: November 2022

Abstract1-nitroso-2-naphthol has thermal instability of thermal decomposition, spontaneous combustion and even explosion. Its thermal decomposition characteristics were tested by synchronous thermal analyzer (TGA/DSC); The activation energy of the thermal decomposition process was calculated by Kissinger method; The infrared absorption characteristic spectra of the gas products produced in the thermal decomposition process were measured by TGA/DSC-FTIR, and the thermal decomposition reaction process was speculated. The results show that the initial temperature (Tonset) of TGA exothermic decomposition of 1-nitroso-2 naphthol is between 129.01 and 155.69 °C, and the faster the heating rate(β), the higher the Tonset, but the faster the thermal decomposition rate, the greater the heat release and the worse the thermal stability. The activation energy (E) of the thermal decomposition process is 83.323 kJ/mol calculated by Kissinger method. The dynamic test results of TGA/DSC-FTIR show that the main reaction of 1-nitroso-2 naphthol during heating is intermolecular dehydration to form ether, and the secondary reaction is decomposition into aliphatic nitro compounds, carbonyl compounds and amines. Sodium hydroxide will reduce the thermal stability of 1-nitroso-2 naphthol. After adding sodium hydroxide, the thermal decomposition process of 1-nitroso-2 naphthol has changed. The main reaction is that 1-nitroso-2-naphthol reacts with sodium hydroxide to produce sodium nitrophenol, which is further decomposed into aliphatic nitro compounds. The research results have guiding significance for finding the reasonable conditions and temperature of 1-nitroso-2 naphthol during storage and transportation.
Millennial-timescale quantitative estimates of climate dynamics in central Europe from earthworm calcite granules in loess deposits
Authors: Prud’homme Charlotte , Fischer Peter , Jöris Olaf , Gromov Sergey , Vinnepand Mathias , Hatté Christine , Vonhof Hubert , Moine Olivier , Vött Andreas , Fitzsimmons Kathryn E. 
Published: November 2022

AbstractIce core and marine archives provide detailed quantitative records of last glacial climate changes, whereas comparable terrestrial records from the mid-latitudes remain scarce. Here we quantify warm season land-surface temperatures and precipitation over millennial timescales for central Europe for the period spanning 45,000–22,000 years before present that derive from two temporally overlapping loess-palaeosol-sequences, dated at high resolution by radiocarbon on earthworm calcite granules. Interstadial temperatures were 1–4 °C warmer than stadial climate, a temperature difference which is strongly attenuated compared to Greenland records. We show that climate in the Rhine Valley was significantly cooler during the warm season and overall drier with annual precipitation values reduced by up to 70% compared to the present day. We combine quantitative estimates with mesoscale wind and moisture transport modelling demonstrating that this region was dominated by westerlies and thereby inextricably linked to North Atlantic climate forcing, although ameliorated.