bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ability as legitimation of tracking: Teachers' representations of students in vocational and academic tracks
Authors: Tarabini Aina , Curran Marta , Castejón Alba 
Published: May 2022

AbstractThe division of educational systems into different tracks—academic and vocational—represents one of the key elements in explaining social stratification and inequalities. Previous research identifies teachers' expectations as a critical factor to understand the relationship between tracking and social inequality. This paper discusses how ability is represented in teachers' discourses and whether and to what extent it works as a legitimation of systemic forms of tracking. Using in‐depth interviews with 35 secondary school tutors, we analyse how teachers draw on the concept of ability to explain students' unequal transitions from a lower comprehensive to an upper tracked education system in Barcelona (Spain). The results indicate three main elements: a highly naturalistic conception of students' abilities among teachers; a remarkably dichotomised conception of theoretical and practical abilities that match with the academic and vocational tracks; and a direct association between types of student and types of track based on different types of ability at a cognitive, behavioural and personal level. Overall, the analysis contributes to opening the ‘black box’ of the notion of ability as represented by teachers and to identifying what we call the ‘mechanisms of misrecognition’ which serve to naturalise, legitimise and reproduce a highly segmented post‐16 school system.
An efficient cell‐free protein synthesis platform for producing proteins with pyrrolysine‐based noncanonical amino acids
Authors: Ranji Charna Arnaz , Des Soye Benjamin J. , Ntai Ioanni , Kelleher Neil L. , Jewett Michael C. 
Published: May 2022

AbstractIncorporation of noncanonical amino acids (ncAAs) into proteins opens new opportunities in biotechnology and synthetic biology. Pyrrolysine (Pyl)‐based ncAAs are some of the most predominantly used, but expression systems suffer from low yields. Here, we report a highly efficient cell‐free protein synthesis (CFPS) platform for site‐specific incorporation of Pyl‐based ncAAs into proteins using amber suppression. This platform is based on cellular extracts derived from genomically recoded Escherichia coli lacking release factor 1 and enhanced through deletion of endonuclease A. To enable ncAA incorporation, orthogonal translation system (OTS) components (i.e., the orthogonal transfer RNA [tRNA] and orthogonal aminoacyl tRNA synthetase) were coexpressed in the source strain prior to lysis and the orthogonal tRNACUAPyl that decodes the amber codon was further enriched in the CFPS reaction via co‐synthesis with the product. Using this platform, we demonstrate production of up to 442 ± 23 µg/mL modified superfolder green fluorescent protein (sfGFP) containing a single Pyl‐based ncAA at high (>95%) suppression efficiency, as well as sfGFP variants harboring multiple, identical ncAAs. Our CFPS platform can be used for the synthesis of modified proteins containing multiple precisely positioned, genetically encoded Pyl‐based ncAAs. We anticipate that it will facilitate more general use of CFPS in synthetic biology.
Changes in the cervicovaginal microbiota composition of HPV16‐infected patients after clinical treatment
Authors: Li Chao , Zhang Zhenbo , Yang Yixia , Liao Hong 
Published: May 2022

AbstractBackgroundHigh‐risk human papillomavirus (hrHPV) infection is a key factor that alters cervicovaginal microbiota patterns and causes cervical intraepithelial neoplasias (CINs) or even cervical cancer. Although local excisional treatment can clear hrHPV infection and restore the cervicovaginal microbiota, it is unclear which cervicovaginal microbiota represents recovery. Our objective was to describe the cervicovaginal microbiota before and after treatments and to assess the association between the microbiota and HPV persistence.ResultsA cohort of 91 participants was classified into four groups (healthy control women and HPV16‐infected women with CIN I, CIN II/III, and squamous cell carcinoma [SCC]). Endocervical swabs were collected 3 months prior to treatment and at 3 months post‐treatment for bacterial 16S rRNA gene pyrosequencing and for HPV DNA testing. There was an increase in the number of Lactobacillus bacterial species present after the clinical treatments, and the community state type (CST) profiles were shifted from dysbiotic CSTs II and IV to Lactobacillus‐dominated CSTs I and III. Specifically, the composition of Geobacter and Prevotella before treatment and Lactobacillus secaliphilus after treatment might have been related to CIN I, the composition of Burkholderia before treatment and Lactobacillus iners after treatment might have been related to CIN II/III, and the composition of Atopobium and Aerococcus before treatment and Bacilli after treatment might have been related to SCC. Further functional predictions revealed that the composition differences were linked to infectious disease‐ and cancer‐related genes.ConclusionOur study provides an illustration of the changes in CSTs and the cervicovaginal microbiota before and after HPV16 clearance in each disease state.
TGIF1 overexpression promotes glioma progression and worsens patient prognosis
Authors: Wang Baoya , Ma Qiong , Wang Xuelin , Guo Kunshan , Liu Zhendong , Li Gang 
Published: May 2022

AbstractTransforming growth factor β‐induced factor homeobox 1 (TGIF1) reportedly promotes the pathological processes of various malignant tumors. However, few studies have investigated the role of TGIF1 in gliomas. We aimed to explore the relationship between TGIF1 expression and the clinical characteristics of patients with glioma, including their overall survival. A total of thousands transcriptome datapoints were downloaded from public databases to determine the correlations between TGIF1 and various clinicopathological features using the Wilcoxon or Kruskal–Wallis tests. The Kaplan–Meier and Cox statistical methods were used to explore the prognostic significance of TGIF1. Gene set enrichment analysis (GSEA) was used to indirectly identify the pathological mechanisms modulated by TGIF1, and compounds that inhibit its expression were determined using a connectivity map (CMap). TGIF1 was significantly overexpressed in gliomas and was correlated with unfavorable prognostic factors and shorter overall survival. Cox analysis confirmed that TGIF1 expression was a significant predictor of poor prognosis in patients with glioma. GSEA revealed that the signaling pathways associated with TGIF1 expression in glioma included extracellular matrix receptor‐ and cell cycle‐modulating proteins. CMap analysis showed that the small molecules scriptaid, torasemide, dexpropranolol, ipratropium bromide, and harmine were potential negative regulators of TGIF1. Finally, in vitro experiments demonstrated that knockdown of TGIF1 significantly inhibited the proliferation and invasion of glioma cell. Taken together, our study, which is the first to comprehensively analyze TGIF1 in gliomas, revealed it to be a novel oncogene in terms of its association with this disease. As such, TGIF1 may be a potential therapeutic target for individualized treatment of patients with glioma.
Stretching and cutting after drilling. Vessel preparation following rotational atherectomy
Authors: Lee Aaron , Kimmelstiel Carey 
Published: May 2022

Key Points
Prior observational studies have suggested that the use of cutting balloon after rotational atherectomy may lead to improved outcomes.
In this meta‐analysis, the authors identified studies that compared patients who had rotational atherectomy followed by additional lesion preparation with either plain balloon or cutting balloon.
Tailoring the therapy to the patient with mitral and tricuspid regurgitation to avoid adverse long‐term outcomes
Authors: Tang Gilbert H. L. , Dangas George D. 
Published: May 2022

Key Points
Tricuspid regurgitation may depend on pulmonary hypertension and may improve after left heart valve replacement or repair
Retrospective studies indicate inferior clinical outcomes with severe untreated/residual tricuspid insufficiency in mutli‐valvular heart disease patients
Early hemodynamic and imaging evaluation of the tricuspid valve insufficiency and evaluation for possible valve repair should be planned after percutaneous left heart valve replacement or repair
In‐hospital mortality and readmission after ST‐elevation myocardial infarction in nonagenarians: A nationwide analysis from the United States
Authors: Ismayl Mahmoud , Machanahalli Balakrishna Akshay , Walters Ryan W. , Pajjuru Venkata S. , Goldsweig Andrew M. , Aboeata Ahmed 
Published: May 2022

AbstractObjectivesTo assess readmission rates in nonagenarians (age ≥ 90 years) with ST‐elevation myocardial infarction (STEMI) following primary percutaneous coronary intervention (pPCI) versus no pPCI.BackgroundThere are limited data exploring readmissions following STEMI in nonagenarians undergoing pPCI versus no pPCI.MethodsWe retrospectively analyzed the Nationwide Readmissions Database to identify nonagenarians hospitalized with STEMI. We divided the cohort into two groups based on pPCI status. We compared mortality during index hospitalization and during 30‐day readmission, readmission rates, and causes of readmissions.ResultsWe identified 58,231 nonagenarian STEMI hospitalizations between 2010 and 2018, of which 18,809 (32.3%) included pPCI, and 39,422 (67.7%) had no pPCI. Unadjusted unplanned 30‐day readmission was higher in pPCI cohort (21.0% vs. 15.4%, p < 0.001). However, mortality during index hospitalization and during 30‐day readmission were significantly lower in pPCI cohort (15.8% vs. 32.2%, p < 0.001; 7.4% vs. 14.2%, p < 0.001, respectively). After adjusting for baseline characteristics, hospitalizations that included pPCI had 25% greater odds of unplanned 30‐day readmission (adjusted odds ratio [aOR]: 1.25, 95% confidence interval [CI]: 1.12–1.39, p < 0.001) and 49% lower odds of in‐hospital mortality during index hospitalization (aOR: 0.51, 95% CI: 0.46–0.56, p < 0.001). Heart failure was the most common cause of readmission in both cohorts followed by myocardial infarction.ConclusionsIn nonagenarians with STEMI, pPCI is associated with slightly higher 30‐day readmission but significantly lower mortality during index hospitalization and during 30‐day readmission than no pPCI. Given the overwhelming mortality benefit with pPCI, further research is necessary to optimize the utilization of pPCI while reducing readmissions following STEMI in nonagenarians.
Preclosure of large bore venous access sites in patients undergoing transcatheter mitral replacement and repair
Authors: Mohammed Mustafa , Nona Paul , Abou Asala Elian , Chiang Michael , Lemor Alejandro , O'Neill Brian , Frisoli Tiberio , Lee James , Wang Dee Dee , O'Neill William W. , Eng Marvin , Villablanca Pedro A. 
Published: May 2022

AbstractObjectiveWe aim to report on the efficacy and safety of large bore venous access (LBVA) preclosure with Perclose™ (Abbott Vascular Devices) suture‐mediated device use following transcatheter edge‐to‐edge (TEER) and replacement (TMVR).BackgroundPatients requiring TEER and TMVR require LBVA. Clinical outcome data on the use of suture‐mediated devices for LBVA site closure are limited.MethodsBetween 2012 and 2019, 354 consecutive high‐risk patients with mitral valvular heart disease underwent TEER (n = 287) with MitraClip and TMVR (n = 67) with Edwards Sapien Valves. Patients had LBVA with 24 or 16 French sheaths. All patients underwent preclosure of LBVA except for one that underwent manual hemostasis.ResultsThere were no closure device failures. None of the cases required surgical repair of the access site following venous preclosure. Two cases had large hematomas (>6 cm) following Perclose in each group. Six cases had small hematomas (<6 cm and >2 cm) with three in each group. There was one major bleeding using Mitral Valve Academic Research Consortium 2 definition (retroperitoneal bleed from arterial puncture) unrelated to the venous closure. Transfusion related to vascular access complication was required in five cases. There were two immediate acute deep venous thromboses postprocedure; one of which occurred after preclosure. There were no arteriovenous malformations, pseudoaneurysms, or access site infections reported following Perclose.ConclusionIn this large sample size analysis, Proglide preclosure technique is a feasible and safe alternative approach to achieving hemostasis after removal of LBVA sheaths in patients undergoing TEER and TMVR. Randomized trials are needed to compare the different modalities of hemostasis.
Choroidal neovascularization in a patient after resolution of multiple evanescent white dot syndrome: A case report
Authors: Burova Marie , Stepanov Alexandr , Almesmary Basma , Jiraskova Nada 
Published: May 2022

AbstractChoroidal neovascularization (CNV) is a rare complication of the Multiple Evanescent White Dot Syndrome (MEWDS). It can develop after resolving of the disease when there is already no evident inflammatory activity. Therefore, a long‐term follow‐up of such patients is important.
Yellow nail syndrome in an elderly sudanese female: A case report
Authors: Ahmed Abdelmuniem , Y. Yousif Mohamed , Abdelmageed Isam , Eljack Moh. Mah. Fadelallah , Abbasher Hussien Mohamed Ahmed Khabab , Tarig AbdAlla Mohamed Malaz , Abdelkareim G. Mohammed Sulieman , B. Salih Elhadi , H. Osman Dina 
Published: May 2022

AbstractYellow nail syndrome is a rare lymphatic abnormality without clear pathogenesis. Hereby, we report a 70‐year‐old Sudanese female patient who presented with recurrent cough, recurrent lower limb swelling, and yellowish nail discoloration diagnosed as yellow nail syndrome but unfortunately passed away due to acute respiratory distress syndrome (ARDS).
Perampanel markedly improved clinical seizures in a patient with a Rett‐like phenotype and 960‐kb deletion on chromosome 9q34.11 including the STXBP1
Authors: Yoshida Syun , Amamoto Masano , Takahashi Tomoyuki , Tomita Ichiro , Yuge Kotaro , Hara Munetsugu , Iwama Kazuhiro , Matsumoto Naomichi , Matsuishi Toyojiro 
Published: May 2022

AbstractIntractable epilepsy was successfully controlled using perampanel, an α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole propionic acid‐type glutamate receptor antagonist, in a 27‐year‐old woman who presented with a Rett syndrome‐like phenotype and novel 960‐kb deletion involving syntaxin‐binding protein 1 on chromosome 9q34.11. Perampanel may be an effective antiepileptic drug for intractable epilepsy associated with STXBP1 mutations.
A case of preeclampsia developing massive ascites after delivery
Authors: Higami Shota , Kondo Emi , Shibata Eiji , Fujimoto Shigeki , Hagimoto Marina , Urakawa Ruka , Matsumiya Tamaki , Uchimura Takayuki , Sakuragi Toshihide , Amimoto Shoko , Kuwazuru Tomoichiro , Mori Hiroshi , Aramaki Satoshi , Yoshino Kiyoshi 
Published: May 2022

AbstractWe experienced a case of preeclampsia in which massive ascites became apparent in the postpartum period. The patient had isolated proteinuria without hypertension before delivery. The infant had fatal growth restriction and neonatal distress. Massive ascites and isolated proteinuria are important symptoms for predicting the aggravation of PE.
Venous thoracic outlet syndrome: When exercising may be discouraged
Authors: Oliveira Inês , Leal Filipa , Santos Lígia , Almeida Pinto João , Nogueira Luis , Mesquita Mari 
Published: May 2022

AbstractThoracic outlet syndrome results from neurovascular compression at the thoracic outlet. Clinical presentation varies according to the predominantly compressed structure, determining its subtype: neurogenic, venous, or arterial. The neurogenic subtype is the most common, affecting 90% of patients, while the vascular subtype is rarely found in practice. We present two case reports of young patients with upper extremity deep vein thrombosis in the setting of venous thoracic outlet syndrome: one due to an anatomic variant, the second an effort thrombosis due to repeated upper arm exercise. These reports depict uncommon clinical scenarios, which imply significant morbidity if not identified and timely treated.
A component could not be larger than the whole, polyclonal immunoglobulin interference in conjugated/direct bilirubin assay and elimination: A case report
Authors: Soleimani Neda , Khaleghpanah Saeideh , Mohammadzadeh Sahand 
Published: May 2022

AbstractAnalytical interferences (hemolysis, icterus, paraproteinemia, and lipemia) are of great concern in laboratory tests. In our case report, the conjugated bilirubin result of a patient was significantly higher than the total bilirubin. We recommend being more cautious with samples that produce indistinguishable results and attempting to resolve the issues before releasing them.
A rare case of pediatric pancreatic pseudocyst
Authors: Jaiswal Vikash , Naz Sidra , Ishak Angela , Batra Nitya , Quinonez Jon , Mukherjee Dattatreya , Pokhrel Nishan Babu 
Published: May 2022

AbstractWe present a case report of a 2‐year‐old boy who presented to a local hospital to evaluate vague abdominal symptoms of one‐month duration. The patient, therefore, had an open cystogastrostomy and drainage of the free abdominal fluid with minimal complications. He was monitored for several days after his surgery.
MNPmApp: An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues
Authors: Potts Catherine , Schearer Julia , Sebrell Thomas A. , Bair Dominic , Ayler Becky , Love Jordan , Dankoff Jennifer , Harris Paul R. , Zosso Dominique , Bimczok Diane 
Published: May 2022

AbstractMononuclear phagocytes (MNPs) such as dendritic cells and macrophages perform key sentinel functions in mucosal tissues and are responsible for inducing and maintaining adaptive immune responses to mucosal pathogens. Positioning of MNPs at the epithelial interface facilitates their access to luminally‐derived antigens and regulates MNP function through soluble mediators or surface receptor interactions. Therefore, accurately quantifying the distribution of MNPs within mucosal tissues as well as their spatial relationship with other cells is important to infer functional cellular interactions in health and disease. In this study, we developed and validated a MATLAB‐based tissue cytometry platform, termed “MNP mapping application” (MNPmApp), that performs high throughput analyses of MNP density and distribution in the gastrointestinal mucosa based on digital multicolor fluorescence microscopy images and that integrates a Monte Carlo modeling feature to assess randomness of MNP distribution. MNPmApp identified MNPs in tissue sections of the human gastric mucosa with 98 ± 2% specificity and 76 ± 15% sensitivity for HLA‐DR+ MNPs and 98 ± 1% specificity and 85 ± 12% sensitivity for CD11c+ MNPs. Monte Carlo modeling revealed that mean MNP‐MNP distances for both HLA‐DR+ and CD11c+ MNPs were significantly lower than anticipated based on random cell placement, whereas MNP‐epithelial distances were similar to randomly placed cells. Surprisingly, H. pylori infection had no significant impact on the number of HLA‐DR and CD11c MNPs or their distribution within the gastric lamina propria. However, our study demonstrated that MNPmApp is a reliable and user‐friendly tool for unbiased quantitation of MNPs and their distribution at mucosal sites.
Differential diagnostic performance of a panel of plasma biomarkers for different types of dementia
Authors: Thijssen Elisabeth H. , Verberk Inge M.W. , Kindermans Jana , Abramian Adlin , Vanbrabant Jeroen , Ball Andrew J. , Pijnenburg Yolande , Lemstra Afina W. , van der Flier Wiesje M. , Stoops Erik , Hirtz Christophe , Teunissen Charlotte E. 
Published: May 2022

AbstractIntroductionWe explored what combination of blood‐based biomarkers (amyloid beta [Aβ]1‐42/1‐40, phosphorylated tau [p‐tau]181, neurofilament light [NfL], glial fibrillary acidic protein [GFAP]) differentiates Alzheimer's disease (AD) dementia, frontotemporal dementia (FTD), and dementia with Lewy bodies (DLB).MethodsWe measured the biomarkers with Simoa in two separate cohorts (n = 160 and n = 152). In one cohort, Aβ1‐42/1‐40 was also measured with mass spectrometry (MS). We assessed the differential diagnostic value of the markers, by logistic regression with Wald's backward selection.ResultsMS and Simoa Aβ1‐42/1‐40 similarly differentiated AD from controls. The Simoa panel that optimally differentiated AD from FTD consisted of NfL and p‐tau181 (area under the curve [AUC] = 0.94; cohort 1) or NfL, GFAP, and p‐tau181 (AUC = 0.90; cohort 2). For AD from DLB, the panel consisted of NfL, p‐tau181, and GFAP (AUC = 0.88; cohort 1), and only p‐tau181 (AUC = 0.81; cohort 2).DiscussionA combination of plasma p‐tau181, NfL, and GFAP, but not Aβ1‐42/1‐40, might be useful to discriminate AD, FTD, and DLB.
Subjective cognitive decline and self‐reported sleep problems: The SCIENCe project
Authors: Exalto Lieza G. , Hendriksen Heleen M.A. , Barkhof Frederik , van den Bosch Karlijn A. , Ebenau Jarith L. , van Leeuwenstijn‐Koopman Mardou , Prins Niels D. , Teunissen Charlotte E. , Visser Leonie N.C. , Scheltens Philip , van der Flier Wiesje M. 
Published: May 2022

AbstractWe aim to investigate the frequency and type of sleep problems in memory clinic patients with subjective cognitive decline (SCD) and their association with cognition, mental health, brain magnetic resonance imaging (MRI), and cerebrospinal fluid (CSF) biomarkers. Three hundred eight subjects (65 ± 8 years, 44% female) were selected from the Subjective Cognitive Impairment Cohort (SCIENCe) project. All subjects answered two sleep questionnaires, Berlin Questionnaire (sleep apnea) and Pittsburgh Sleep Quality Index (sleep quality) and underwent a standardized memory clinic work‐up. One hundred ninety‐eight (64%) subjects reported sleep problems, based on 107 (35%) positive screenings on sleep apnea and 162 (53%) on poor sleep quality. Subjects with sleep problems reported more severe depressive symptoms, more anxiety, and more severe SCD. Cognitive tests, MRI, and CSF biomarkers did not differ between groups. Our results suggest that improvement of sleep quality and behaviors are potential leads for treatment in many subjects with SCD to relieve the experienced cognitive complaints.
Distinguishing the papilla of Vater during biliary cannulation using texture and color enhancement imaging: A pilot study
Authors: Miyaguchi Kazuya , Mizuide Masafumi , Tanisaka Yuki , Fujita Akashi , Jinushi Ryuhei , Hiromune Katsuda , Ogawa Tomoya , Saito Yoichi , Tashima Tomoaki , Mashimo Yumi , Imaeda Hiroyuki , Ryozawa Shomei 
Published: May 2022

AbstractObjectivesUnderstanding the exact morphology of the bile duct opening is important for determining the success of bile duct cannulation. Texture and color enhancement imaging (TXI) has been reported to enhance slight changes in color tone and structure that are difficult to see with white light imaging. This study investigated whether TXI mode1 could improve papillary recognition by trainees inexperienced in endoscopic retrograde cholangiopancreatography.MethodsWe included 31 patients with naive papilla of Vater at a single institution in the study. Trainee endoscopists (n = 4) evaluated and identified the papilla according to the Inomata classification using white light imaging and TXI. The degree of agreement with the evaluation of supervising physicians (n = 4) was examined using the McNemar test.ResultsIn the trainee group, the kappa coefficient agreements were κ = 0.346 and κ = 0.754 for white light imaging and TXI, respectively. When further evaluated, the separate and septal types of papilla groups showed an increased concordance rate in one of the four trainees (76.67%–96.67%, p = 0.031, respectively). Moreover, comparison for two‐group evaluation showed an increased kappa coefficient in two of four trainees (0.34–0.92, p = 0.010, 0.45–0.92, p = 0.024).ConclusionsObservation of the duodenal papilla using TXI improved papillary differentiation and suggested the potential of TXI as a clinical tool. Further study of this method is necessary; it is expected to help reduce cannulation time and the incidence of pancreatitis.