bluuebunny/crossref_metadata_2025_split huggingface dataset:
Push‐out bond strength of the calcium silicate‐based endodontic cements in the presence of blood: A systematic review and meta‐analysis of in vitro studies
Authors: Alipour Mahdieh , Faraji Gavgani Leili , Ghasemi Negin 
Published: February 2022

AbstractObjectivesThe push‐out bond strength (POBS) of calcium silicate‐based cements (CSCs) to the dentinal wall is considered one of the essential physical properties for clinical success. The presence of blood in the treatment area affects the POBS of these types of cement. This study aimed to evaluate the impact of blood contamination on the bond strength of CSCs and dentinal walls.Material and MethodsThis systematic review was performed by searching electronic databases (MEDLINE‐PubMed, Scopus, and EMBASE) to include relevant in vitro studies published between 1992 and April 2020. Two reviewers independently evaluated the selected studies and extracted data on the type of studied CSCs, evaluated area of the teeth, sample size, the dimension of a prepared area, slice thickness, storage duration, the setting of the universal testing machine (UTM), effects of blood contamination on POBS of CSCs and their failure modes. The bond strength of evaluated CSCs in studies was used for network meta‐analysis.ResultsInitial searches identified 292 articles, while only 13 articles met the inclusion criteria. Full texts of these articles were evaluated, and data extraction was performed. The effect of blood contamination on bond strength to the dentinal wall was assessed in various CSCs such as PMTA, Biodentine, and AMTA. The network meta‐analysis results showed that the bond strength of Biodentine was significantly higher than other types of cement in blood presence (p < .05).ConclusionsBased on the current systematic review, despite controversies among the result of the different articles and the lack of data for some CSCs like bioaggregate, it could be concluded that the bond strength of Biodentine to the dentinal wall is better than other evaluated CSCs in the presence of blood.
Impaired tongue motor control after temporomandibular disorder: A proof‐of‐concept case‐control study of tongue print
Authors: Alvarado Caroline , Arminjon Audrey , Damieux‐Verdeaux Clovis , Lhotte Claire , Condemine Chloé , Cousin Anne‐Sabine , Sigaux Nicolas , Bouletreau Pierre , Mateo Sébastien 
Published: February 2022

AbstractBackgroundTemporomandibular disorder (TMD) perturbs the tongue motor control and consequently impairs oral function, but strength training reduces this impairment. However, tongue motor control is widely reduced to a matter of strength.ObjectivesTo investigate the accuracy of the tongue placement as a measure of tongue motor control in patients with TMD compared with age‐ and sex‐matched healthy participants.Material and MethodsThis proof‐of‐concept case‐control study was prospective, observational, and part of the TMIQ study (NCT04102306). After pointing against a wood stick while maintaining the tongue as sharp as possible, the examinator drew the contour of the tongue print on the wood stick, which was then scanned for image analyses to compute the area for each participant using ImageJ.ResultsA total of 94 participants were included, all patients with TMD (n = 47) diagnosed with myalgia, 61% with intra‐articular joint disorder accordingly to the DC/TMD. The median (IQR) tongue print area was 117 (111) mm2 for the TMD group and 93.5 (76.2) mm2 for the control group (V = 352, p = .04) and the median [95% confidence interval] difference was 25.4 [1.3; 51.0] mm². Overlapping of the 95% confidence intervals of the area evidenced no significant difference between the categories of the DC/TMD. The corrected each area–total correlation (r = .24) suggests a reasonably homogenous thus valid measure.ConclusionThe results suggest that TMD impairs the motor control of the tongue. Therefore, the sharpest tongue pointing test may constitute a simple and accessible clinical tool to assess the accuracy of tongue placement in TMD patients. The study was registered on ClinicalTrial.gov with identification number NCT04102306.
Hydrogen Spillover‐Enhanced Heterogeneously Catalyzed Hydrodeoxygenation for Biomass Upgrading
Authors: Geng Yanyan , Li Hao 
Published: March 2022

AbstractHydrodeoxygenation (HDO) is regarded as a promising technology for biomass upgrading to obtain sustainable and competitive chemicals and fuels. In fact, biomass HDO over heterogeneous solid catalysts is often accompanied by the phenomenon of hydrogen spillover, which further affects the catalytic performance. Thus, it is necessary to gain in‐depth understand the promoting effect of hydrogen spillover in the biomass HDO process to obtain desired conversion and selectivity. This Review summarized the extensive research on hydrogen spillover in biomass refining and discussed in detail the regulation mechanism of hydrogen spillover in biomass HDO process, mainly by regulating different active center sites on catalyst supports, such as metal sites, acid sites, surface functional groups, and defective sites, which exhibit independent and synergistic characteristics promoting catalyst activity, selectivity, and stability. Finally, the prospective of hydrogen spillover in biomass HDO applications was critically evaluated, and the key technical challenges in developing “hydrogen‐free” HDO and upgrading biofuels were highlighted. The presentation of hydrogen spillover‐enhanced catalytic biomass HDO in this Review will hopefully provide insight and guidance for further development of efficient catalysts and preparation of high‐value chemicals in the future.
Melatonin enhances osteoblastogenesis of senescent bone marrow stromal cells through NSD2‐mediated chromatin remodelling
Authors: Xie Ying , Han Na , Li Feng , Wang Lijuan , Liu Gerui , Hu Meilin , Wang Sheng , Wei Xuelei , Guo Jing , Jiang Hongmei , Wang Jingjing , Li Xin , Wang Yixuan , Wang Jingya , Bian Xiyun , Zhu Zhongjiao , Zhang Hui , Liu Chunhua , Liu Xiaozhi , Liu Zhiqiang 
Published: February 2022

AbstractBackgroundAging‐associated osteoporosis is frequently seen in the elderly in clinic, but efficient managements are limited because of unclear nosogenesis. The current study aims to investigate the role of melatonin on senescent bone marrow stromal cells (BMSCs) and the underlying regulating mechanism.MethodsMelatonin levels were tested by ELISA. Gene expression profiles were performed by RNA‐sequencing, enrichment of H3K36me2 on gene promoters was analyzed by Chromatin Immunoprecipitation Sequencing (ChIP‐seq), and chromatin accessibility was determined by Assay for Transposase‐Accessible Chromatin with high‐throughput sequencing (ATAC‐seq). Osteogenesis of BMSCs in vitro was measured by Alizarin Red and Alkaline Phosphatase staining, and in vivo effects of melatonin was assessed by histological staining and micro computed tomography (micro‐CT) scan. Correlation of NSD2 expression and severity of senile osteoporosis patients were analyzed by Pearson correlation.ResultsMelatonin levels were decreased during aging in human bone marrow, accompanied by downregulation of the histone methyltransferase nuclear receptor binding SET domain protein 2 (NSD2) expression in the senescent BMSCs. Melatonin stimulated the expression of NSD2 through MT1/2‐mediated signaling pathways, resulting in the rebalancing of H3K36me2 and H3K27me3 modifications to increase chromatin accessibility of the osteogenic genes, runt‐related transcription factor 2 (RUNX2) and bone gamma‐carboxyglutamate protein (BGLAP). Melatonin promoted osteogenesis of BMSCs in vitro, and alleviates osteoporosis progression in the aging mice. In clinic, severity of senile osteoporosis (SOP) was negatively correlated with melatonin level in bone marrow, as well as NSD2 expression in BMSCs. Similarly, melatonin remarkably enhanced osteogenic differentiation of BMSCs derived from SOP patients in vitro.ConclusionsCollectively, our study dissects previously unreported mechanistic insights into the epigenetic regulating machinery of melatonin in meliorating osteogenic differentiation of senescent BMSC, and provides evidence for application of melatonin in preventing aging‐associated bone loss.
Tumour cells are sensitised to ferroptosis via RB1CC1‐mediated transcriptional reprogramming
Authors: Xue Xiangfei , Ma Lifang , Zhang Xiao , Xu Xin , Guo Susu , Wang Yikun , Qiu Shiyu , Cui Jiangtao , Guo Wanxin , Yu Yongchun , Sun Fenyong , Shi Yi , Wang Jiayi 
Published: February 2022

AbstractBackgroundFerroptosis, a form of regulated cell death, is an important topic in the field of cancer research. However, the signalling pathways and factors that sensitise tumour cells to ferroptosis remain elusive.MethodsWe determined the level of ferroptosis in cells by measuring cell death and lipid reactive oxygen species (ROS) production. The expression of RB1‐inducible coiled‐coil 1 (RB1CC1) and related proteins was analyzed by immunoblotting and immunohistochemistry. Immunofluorescence was used to determine the subcellular localization of RB1CC1. We investigated the mechanism of RB1CC1 nuclear translocation by constructing a series of RB1CC1 variants. To examine the ferroptosis‐ and RB1CC1‐dependent transcriptional program in tumour cells, chromatin immunoprecipitation sequencing was performed. To assess the effect of c‐Jun N‐terminal kinase (JNK) agonists on strenthening imidazole ketone erastin (IKE) therapy, we constructed cell‐derived xenograft mouse models. Mouse models of hepatocellular carcinoma to elucidate the importance of Rb1cc1 in IKE‐based therapy of liver tumourigenesis.ResultsRB1CC1 is upregulated by lipid ROS and that nuclear translocation of phosphorylation of RB1CC1 at Ser537 was essential for sensitising ferroptosis in tumour cells. Upon ferroptosis induction, nuclear RB1CC1 sharing forkhead box (FOX)‐binding motifs recruits elongator acetyltransferase complex subunit 3 (ELP3) to strengthen H4K12Ac histone modifications within enhancers linked to ferroptosis. This also stimulated transcription of ferroptosis‐associated genes, such as coiled‐coil–helix–coiled‐coil–helix domain containing 3 (CHCHD3), which enhanced mitochondrial function to elevate mitochondrial ROS early following induction of ferroptosis. FDA‐approved JNK activators reinforced RB1CC1 nuclear translocation and sensitised cells to ferroptosis, which strongly suggested that JNK is upstream of RB1CC1. Nuclear localisation of RB1CC1 correlated with lipid peroxidation in clinical lung cancer specimens. Rb1cc1 was essential for ferroptosis agonists to suppress liver tumourigenesis in mice.ConclusionsOur findings indicate that RB1CC1‐associated signalling sensitises tumour cells to ferroptosis and that targeting RB1CC1 may be beneficial for tumour treatment.
Maternal deprivation and milk replacement affect the integrity of gray and white matter in the developing lamb brain
Authors: Love Scott A. , Haslin Emmanuelle , Bellardie Manon , Andersson Frédéric , Barantin Laurent , Filipiak Isabelle , Adriaensen Hans , Fazekas Csilla L. , Leroy Laurène , Zelena Dóra , Morisse Mélody , Elleboudt Frédéric , Moussu Christian , Lévy Frédéric , Nowak Raymond , Chaillou Elodie 
Published: February 2022

AbstractThe psychoendocrine evaluation of lamb development has demonstrated that maternal deprivation and milk replacement alters health, behavior, and endocrine profiles. While lambs are able to discriminate familiar and non‐familiar conspecifics (mother or lamb), only lambs reared with their mother develop such clear social discrimination or preference. Lambs reared without mother display no preference for a specific lamb from its own group. Differences in exploratory and emotional behaviors between mother‐reared and mother‐deprived lambs have also been reported. As these behavioural abilities are supported by the brain, we hypothesize that rearing with maternal deprivation and milk replacement leads to altered brain development and maturation. To test this hypothesis, we examined brain morphometric and microstructural variables extracted from in vivo T1‐weighted and diffusion‐weighted magnetic resonance images acquired longitudinally (1 week, 1.5 months, and 4.5 months of age) in mother‐reared and mother‐deprived lambs. From the morphometric variables the caudate nuclei volume was found to be smaller for mother‐deprived than for mother‐reared lambs. T1‐weighted signal intensity and radial diffusivity were higher for mother‐deprived than for mother‐reared lambs in both the white and gray matters. The fractional anisotropy of the white matter was lower for mother‐deprived than for mother‐reared lambs. Based on these morphometric and microstructural characteristics we conclude that maternal deprivation delays and affects lamb brain growth and maturation.
Inhibition Effect of Oryzanol on the Degradation of Tocopherol and the Oxidation Kinetic of Rice Bran Oils with Different Content of Oryzanol and Tocopherol
Authors: Huang Jianhua , Chen Chuying , Zhao Zhengmei , Li Xu , Wang Xingguo , Jin Qingzhe , Tang Junjun 
Published: February 2022

AbstractThe purpose of this study is to investigate the inhibitory effect of oryzanol on the degradation of tocopherol during heating of rice bran oil at 110 and 170 °C and the oxidation kinetics of rice bran oil. Results demonstrate the degradation of oryzanol and tocopherol in rice bran oils follow the first‐order kinetic models. The higher concentration of oryzanol has no inhibitory effect on the degradation of tocopherol when the concentration of tocopherol in rice bran oil is 500 mg kg−1. However, when the concentrations of tocopherol reach 1500 and 2000 mg kg−1, higher oryzanol concentration make the inhibitory effects on the degradation of tocopherol more pronounced. Compared with rice bran oils containing lower levels of tocopherol and oryzanol, rice bran oils containing higher levels of tocopherol and oryzanol have higher activation energy (Ea), activation enthalpy (ΔH), and activation entropy (ΔS) and a larger temperature acceleration factor (Q10), which indicate that higher levels of oryzanol and tocopherol promote the thermal oxidation of rice bran oil. Therefore, in the compound application of tocopherol and oryzanol in rice bran oil, the influence of its content on the thermal oxidation of oil should be fully considered.Practical Application: This study may be useful for the grain and oil industry because studies on the degradation kinetics of oryzanol and tocopherol during heating of rice bran oil and oxidation kinetics of rice bran oil are still lacking in the literature. The results of this study is valuable for predicting and evaluating the oxidation process and nutritional properties of rice bran oils under various thermal oxidation conditions.
Palladium‐Catalyzed C(sp2/sp3)−H Arylation of Aryl Glycinamide Derivatives Using Picolinamide as Directing Group
Authors: Martínez‐Flores Sebastián , Mujica‐Martinez Cesar A. , Polindara‐García Luis A. 
Published: March 2022

AbstractA Pd(II)‐catalyzed C(sp2/sp3)−H bond arylation protocol has been developed to access ortho‐diaryl, ortho‐mono‐aryl, and biaryl‐diarylmethane‐glycinamide derivatives bearing picolinamide as directing group. Mechanistic details of the process have been obtained by computational modelling of the activation reaction through DFT calculations.
Observation of Mercury Underpotential Deposition on an Ir Surface using the Electrochemical Quartz Crystal Microbalance Technique
Authors: Nagai Tsukasa , Siroma Zyun , Akita Tomoki , Ioroi Tsutomu 
Published: March 2022

AbstractDevelopment of a technique to measure electrochemical surface area (ECSA) is essential to evaluate the activity and durability of Ir‐based catalysts for polymer electrolyte membrane (PEM) water electrolysis. Mercury underpotential deposition (Hg‐UPD) on Ir and IrOx in perchloric acid has been reported to be useful for ECSA evaluation. However, previous methods need to be carefully evaluated for reproducibility due to the presence of Hg(II)/Hg(I) redox reactions, and differences in UPD potentials between studies. Here, the electrochemical behavior of an Ir electrode in a mercury nitrate containing solution was investigated using the electrochemical quartz crystal microbalance method. We confirmed that a Hg adatom is reversibly adsorbed and desorbed on an Ir surface at potentials over 0.7 V vs the reversible hydrogen electrode in both perchloric acid and sulfuric acid. These results contribute to the establishment of a reproducible method for ECSA calculations for Ir‐based catalysts.
Palaeoproterozoic S‐type granites from Garhwal Himalaya, NW India: Geochemistry, Sm–Nd isotope systematics and tectonic implications
Authors: Mishra Sumit , Slabunov Alexander I. , Nainwal Harish C. , Singh Vinod K. , Singh Pradip K. , Nesterova Natalia S. , Svetov Sergei A. 
Published: March 2022

A combined geochemical and Sm–Nd isotopic study on the Palaeoproterozoic (1,845 Ma) granites of the Lesser Himalayan Crystalline Sequence (LHCS) in the Garhwal region of NW India has been done in the present study. These granite samples are characterized by high silica, alumina, and potash and belong to a peraluminous to strongly peraluminous series, having molar A/CNK values of 1.01 to 2.4. The low P2O5 contents and its negative correlation with SiO2 presiding out that the granites have S‐type affinity, also supported by various classification diagrams (ACF; SiO2 vs. P2O5, Na2O + K2O‐CaO, and Th). The concentration of trace elements Ba, Sr, Nb, and Ti are low, and Rb, Th, U, and Pb are found to be high. The granites have low total rare earth elements contents of 56.19–229.16 ppm with enrichment in Light rare earth elements (LREE) ([La/Yb]N = 1.61–15.08) and negative europium anomaly (Eu/Eu* = 0.12–0.31). Sm–Nd isotope studies were also performed for three granite samples. Estimated model ages as 2.5–2.7 Ga, indicates the contribution of the Archean crustal substrate as their protolith source. Therefore, we assume that the melting of metasedimentary rocks with Archean protolith can form these peraluminous granites in an accretional–collisional event, during the Palaeoproterozoic on the western flank of the Columbia supercontinent.
Ethnicity, disease severity, and survival in Canadian patients with primary biliary cholangitis
Authors: Roberts Surain B. , Hirschfield Gideon M. , Worobetz Lawrence J. , Vincent Catherine , Flemming Jennifer A. , Cheung Angela , Qumosani Karim , Swain Mark , Grbic Dusanka , Ko Hin Hin , Peltekian Kevork , Selzner Nazia , Abrahamyan Lusine , Aziz Bishoi , Lytvyak Ellina , Tirona Kattleya , Gulamhusein Aliya F. , Janssen Harry L. A. , Montano‐Loza Aldo J. , Mason Andrew L. , Hansen Bettina E. ,   the Canadian Network for Autoimmune Liver Disease (CaNAL)
Published: February 2022

Abstract

Background and Aims
We investigated associations between ethnicity, survival, and disease severity in a diverse Canadian cohort of patients with primary biliary cholangitis (PBC).

Approach and Results
Patients with PBC were included from the Canadian Network for Autoimmune Liver Disease. Ethnicity was defined using a modified list adopted from Statistics Canada, and ethnicities with small samples were grouped. Clinical events were defined as liver decompensation, HCC, liver transplantation, or death. Clinical event–free and liver transplantation–free survival were analyzed using Cox regression. Trajectories of serum liver function tests were assessed over time using mixed‐effects regression. Health‐related quality of life was assessed using the Short Form 36, the PBC‐40 questionnaire, and the 5‐D Itch scale and analyzed using mixed‐effects regression. The cohort included 1538 patients with PBC from six sites and was comprised of 82% White, 4.7% Indigenous, 5.5% East Asian, 2.6% South Asian, and 5.1% miscellaneous ethnicities. Indigenous patients were the only ethnic group with impaired liver transplant–free and event‐free survival compared to White patients (HR, 3.66; 95% CI, 2.23–6.01; HR, 3.09; 95% CI, 1.94–4.92). Indigenous patients were more likely to have a clinical event before diagnosis (10%) than all other ethnic groups despite similar age at diagnosis. Indigenous patients presented with higher alkaline phosphatase, total bilirubin, and GLOBE scores than White patients; and these relative elevations persisted during follow‐up.

Conclusions
Indigenous Canadians with PBC present with advanced disease and have worse long‐term outcomes compared to White patients.
Metabolic subtypes of patients with NAFLD exhibit distinctive cardiovascular risk profiles
Authors: Martínez‐Arranz Ibon , Bruzzone Chiara , Noureddin Mazen , Gil‐Redondo Ruben , Mincholé Itziar , Bizkarguenaga Maider , Arretxe Enara , Iruarrizaga‐Lejarreta Marta , Fernández‐Ramos David , Lopitz‐Otsoa Fernando , Mayo Rebeca , Embade Nieves , Newberry Elizabeth , Mittendorf Bettina , Izquierdo‐Sánchez Laura , Smid Vaclav , Arnold Jorge , Iruzubieta Paula , Pérez Castaño Ylenia , Krawczyk Marcin , Marigorta Urko M. , Morrison Martine C. , Kleemann Robert , Martín‐Duce Antonio , Hayardeny Liat , Vitek Libor , Bruha Radan , Aller de la Fuente Rocío , Crespo Javier , Romero‐Gomez Manuel , Banales Jesus M , Arrese Marco , Cusi Kenneth , Bugianesi Elisabetta , Klein Samuel , Lu Shelly C. , Anstee Quentin M. , Millet Oscar , Davidson Nicholas O. , Alonso Cristina , Mato José M. 
Published: February 2022

Abstract

Background and Aims
We previously identified subsets of patients with NAFLD with different metabolic phenotypes. Here we align metabolomic signatures with cardiovascular disease (CVD) and genetic risk factors.

Approach and Results
We analyzed serum metabolome from 1154 individuals with biopsy‐proven NAFLD, and from four mouse models of NAFLD with impaired VLDL‐triglyceride (TG) secretion, and one with normal VLDL‐TG secretion. We identified three metabolic subtypes: A (47%), B (27%), and C (26%). Subtype A phenocopied the metabolome of mice with impaired VLDL‐TG secretion; subtype C phenocopied the metabolome of mice with normal VLDL‐TG; and subtype B showed an intermediate signature. The percent of patients with NASH and fibrosis was comparable among subtypes, although subtypes B and C exhibited higher liver enzymes. Serum VLDL‐TG levels and secretion rate were lower among subtype A compared with subtypes B and C. Subtype A VLDL‐TG and VLDL–apolipoprotein B concentrations were independent of steatosis, whereas subtypes B and C showed an association with these parameters. Serum TG, cholesterol, VLDL, small dense LDL5,6, and remnant lipoprotein cholesterol were lower among subtype A compared with subtypes B and C. The 10‐year high risk of CVD, measured with the Framingham risk score, and the frequency of patatin‐like phospholipase domain‐containing protein 3 NAFLD risk allele were lower in subtype A.

Conclusions
Metabolomic signatures identify three NAFLD subgroups, independent of histological disease severity. These signatures align with known CVD and genetic risk factors, with subtype A exhibiting a lower CVD risk profile. This may account for the variation in hepatic versus cardiovascular outcomes, offering clinically relevant risk stratification.