bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteome Analysis of Vacuoles Isolated from Fig (Ficus carica L.) Flesh during Fruit Development
Authors: Kuang Liuqing , Chen Shangwu , Guo Yan , Scheuring David , Flaishman Moshe A , Ma Huiqin 
Published: March 2022

Abstract
Fruit flesh cell vacuoles play a pivotal role in fruit growth and quality formation. In the present study, intact vacuoles were carefully released and collected from protoplasts isolated from flesh cells at five sampling times along fig fruit development. Label-free quantification and vacuole proteomic analysis identified 1,251 proteins, 1,137 of which were recruited as differentially abundant proteins (DAPs) by fold change ≥ 1.5, P &lt; 0.05. DAPs were assigned to 10 functional categories; among them, 238, 186, 109, 93 and 90 were annotated as metabolism, transport proteins, membrane fusion or vesicle trafficking, protein fate and stress response proteins, respectively. Decreased numbers of DAPs were uncovered along fruit development. The overall changing pattern of DAPs revealed two major proteome landscape conversions in fig flesh cell vacuoles: the first occurred when fruit developed from late-stage I to mid-stage II, and the second occurred when the fruit started ripening. Metabolic proteins related to glycosidase, lipid and extracellular proteins contributing to carbohydrate storage and vacuole expansion, and protein-degrading proteins determining vacuolar lytic function were revealed. Key tonoplast proteins contributing to vacuole expansion, cell growth and fruit quality formation were also identified. The revealed comprehensive changes in the vacuole proteome during flesh development were compared with our previously published vacuole proteome of grape berry. The information expands our knowledge of the vacuolar proteome and the protein basis of vacuole functional evolution during fruit development and quality formation.
Mitochondrion-encoded circular RNAs are widespread and translatable in plants
Authors: Liao Xun , Li Xiao-Jie , Zheng Guan-Tao , Chang Feng-Rui , Fang Lin , Yu Hang , Huang Jun , Zhang Ya-Feng 
Published: March 2022

AbstractNucleus-encoded circular RNAs (ncircRNAs) have been widely detected in eukaryotes, and most circRNA identification algorithms are designed to identify them. However, using these algorithms, few mitochondrion-encoded circRNAs (mcircRNAs) have been identified in plants, and the role of plant mcircRNAs has not yet been addressed. Here, we developed a circRNA identification algorithm, mitochondrion-encoded circRNA identifier, based on common features of plant mitochondrial genomes. We identified 7,524, 9,819, 1,699, 1,821, 1,809, and 5,133 mcircRNAs in maize (Zea mays), Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), tomato (Solanum lycopersicum), cucumber (Cucumis sativus), and grape (Vitis vinifera), respectively. These mcircRNAs were experimentally validated. Plant mcircRNAs had distinct characteristics from ncircRNAs, and they were more likely to be derived from RNA degradation but not intron backsplicing. Alternative circularization was prevalent in plant mitochondria, and most parental genomic regions hosted multiple mcircRNA isoforms, which have homogenous 5′ termini but heterogeneous 3′ ends. By analysis of mitopolysome and mitoribosome profiling data, 1,463 mcircRNAs bound to ribosomes were detected in maize and Arabidopsis. Further analysis of mass spectrometry-based proteomics data identified 358 mcircRNA-derived polypeptides. Overall, we developed a computational pipeline that efficiently identifies plant mcircRNAs, and we demonstrated mcircRNAs are widespread and translated in plants.
Translating Outcomes from the Clinical Setting to Preclinical Models: Chronic Pain and Functionality in Chronic Musculoskeletal Pain
Authors: Lenert Melissa E , Gomez Rachelle , Lane Brandon T , Dailey Dana L , Vance Carol G T , Rakel Barbara A , Crofford Leslie J , Sluka Kathleen A , Merriwether Ericka N , Burton Michael D 
Published: March 2022

Abstract
Fibromyalgia (FM) is a chronic pain disorder characterized by chronic widespread musculoskeletal pain (CWP), resting pain, movement-evoked pain (MEP), and other somatic symptoms that interfere with daily functioning and quality of life. In clinical studies, this symptomology is assessed, while preclinical models of CWP are limited to nociceptive assays. The aim of the study was to investigate the human-to-model translatability of clinical behavioral assessments for spontaneous (or resting) pain and MEP in a preclinical model of CWP. For preclinical measures, the acidic saline model of FM was used to induce widespread muscle pain in adult female mice. Two intramuscular injections of acidic or neutral pH saline were administered following baseline measures, 5 days apart. An array of adapted evoked and spontaneous pain measures and functional assays were assessed for 3 weeks. A novel paradigm for MEP assessment showed increased spontaneous pain following activity. For clinical measures, resting and movement-evoked pain and function were assessed in adult women with FM. Moreover, we assessed correlations between the preclinical model of CWP and in women with fibromyalgia to examine whether similar relationships between pain assays that comprise resting and MEP existed in both settings. For both preclinical and clinical outcomes, MEP was significantly associated with mechanical pain sensitivity. Preclinically, it is imperative to expand how the field assesses spontaneous pain and MEP when studying multi-symptom disorders like FM. Targeted pain assessments to match those performed clinically is an important aspect of improving preclinical to clinical translatability of animal models.
Effectiveness of Pharmacological-Based Interventions, Including Education and Prescribing Strategies, to Reduce Subacute Pain After Total Hip or Knee Arthroplasty: A Systematic Review of Randomized Controlled Trials
Authors: Liu Shania , Genel Furkan , Harris Ian A , Patanwala Asad E , Adie Sam , Stevens Jennifer , Hassett Geraldine , Luckie Kate , Penm Jonathan , Naylor Justine 
Published: March 2022

Abstract

Background
Total knee arthroplasty (TKA) and total hip arthroplasty (THA) surgeries are among the most common elective procedures. Moderate to severe postoperative pain during the subacute period (defined here as the period from hospital discharge to 3 months postoperatively) is a predictor of persistent pain 12 months postoperatively. This review aimed to examine the available postdischarge pharmacological interventions, including educational and prescribing strategies, and their effect on reducing pain during the subacute period after TKA or THA.

Methods
We searched seven electronic databases from inception to April 22, 2021. Published randomized controlled trials of adults who underwent TKA or THA and received a pharmacological-based intervention commencing within 1 week after hospital discharge and conducted for up to 3 months postoperatively were compared with any treatment. Two reviewers independently extracted data on the primary outcome, pain intensity. This review was registered prospectively on PROSPERO (ID: CRD42021250384).

Results
Four trials involving 660 participants were included. Interventions included changing analgesic prescribing practices upon hospital discharge and education on analgesic use. Providing multimodal non-opioid analgesia in addition to reduced opioid quantity was associated with lower subacute pain (coefficient –0.81; 95% confidence interval –1.33 to –0.29; P = 0.003). Education on analgesic use during multidisciplinary home visits was effective for reducing pain intensity during the subacute period (6.25 ± 10.13 vs 35.67 ± 22.05; P &lt; 0.001) compared with usual care.

Conclusions
Interventions involving the provision of multimodal non-opioid analgesia and education on analgesic use show positive effects on reducing pain intensity during the subacute period after TKA and THA.
Soluble LILRA3 is aberrantly expressed in antiphospholipid syndrome (APS) and is a potential marker of thrombotic APS
Authors: Liu Hongjiang , Li Chun , Shi Hui , Guo Yixue , Tang Yundi , Chen Chen , Zhao Zhen , Hoy Claire K , Yalavarthi Srilakshmi , Figueroa-Parra Gabriel , Duarte-Garcia Ali , Zuo Yu , Li Zhanguo , Knight Jason S , Guo Jianping 
Published: March 2022

Abstract

Objective
Leucocyte immunoglobulin-like receptor A3 (LILRA3) belongs to a family of leucocyte receptors. Our previous study reported LILRA3 transcripts were markedly upregulated in neutrophils from patients with APS. We undertook this study to investigate clinical implications of LILRA3 in APS and its potential role in APS-associated thrombosis.

Methods
Two independent cohorts were studied. The first consisted of 294 APS patients, 48 asymptomatic aPL carriers and 150 healthy controls (HCs) from Peking University People’s Hospital. The second included 99 APS patients, 25 aPL carriers and 40 HCs from United States APS centres. Serum or plasma concentrations of LILRA3 and MPO-DNA complexes were measured. Additionally, 35 patients with thrombotic APS (tAPS) were evaluated to determine potential effects of immunosuppressive therapy on serum concentrations of LILRA3 and MPO-DNA complexes.

Results
Both positivity and serum concentration of LILRA3 were significantly increased in APS patients, especially in those with tAPS. LILRA3-positive tAPS patients displayed more severe thrombotic manifestations. Serum LILRA3 was positively correlated with MPO-DNA complexes in LILRA3-positive tAPS. After immunosuppressive treatment, LILRA3 and MPO-DNA complexes were consistently decreased in tAPS patients. Key findings from the Peking cohort were confirmed in the United States cohort.

Conclusion
Our study provides first evidence that LILRA3 is aberrantly expressed in APS, especially in patients with tAPS. Serum LILRA3 correlated with MPO-DNA complexes, and the two indices were consistently decreased in tAPS patients after treatment. LILRA3 may play a role in thrombosis of APS and may serve as a biomarker and/or therapeutic target in tAPS.
DIRECT ASSIMILATION OF ATMOSPHERIC CARBON BY IMMATURE APPLE FRUITS
Authors: Imada Shogo , Tako Yasuhiro , Moriya Yuki 
Published: March 2022

Abstract
Although fruit development primarily depends on photoassimilation by leaves, immature green fruits can also directly assimilate atmospheric CO2. To elucidate the process of C accumulation due to direct assimilation by fruit, we conducted a 13CO2 exposure experiment in an orchard in late June with immature ‘Fuji’ apples (Malus domestica). Four fruits from three trees were enclosed in transparent plastic bags and exposed to 13CO2 using an in-situ exposure system. Fruits were collected prior to and immediately following exposure in early July, late September and mid-November, and 13C concentrations in the peduncle, skin, flesh and core (including seeds) were measured. The higher assimilated 13C concentrations measured following exposure indicated that the fruits directly assimilated atmospheric 13C. The 13C concentration in fruit skin was higher immediately after exposure and in early July compared with that prior to exposure. In late September and mid-November, 13C concentrations were close to natural levels.
Adoption of LGBT-Inclusive Policies: Social Construction, Coercion, or Competition?
Authors: Gardberg Naomi A , Newburry William , Hudson Bryant A , Viktora-Jones Magdalena 
Published: March 2022

Abstract
Companies evaluate LGBT policy adoption in an environment with competing and often contradictory societal institutions and ethical frames. This makes the adoption process more difficult to understand when compared to new practice diffusion in less contested settings, providing an opportunity to examine diffusion in an uncertain and varying institutional environment. Herein, we develop a policy adoption model that examines both competing and reinforcing forces. Utilizing a longitudinal dataset of LGBT policy adoption by 283 firms across 1980 firm-years between 2002 and 2014 as measured by the Human Rights Campaign (HRC), we find that firms respond to coercive, social constructivist, and competitive forces for and against LGBT-inclusive work policy adoption. We find that coercive forces exercised by shareholder resolutions and competitive forces driven by industry-level policy adoption lead to firm-level policy adoption. However, other forces, such as state-level anti-marriage equality constitutional amendments, are associated with LGBT-exclusive policies. We also disaggregate the overall HRC policy data into equal employment opportunity (EEO) policy, benefits, and inclusion dimensions and find similarities and differences among our hypothesized relationships.
Enduring Consequences of Dehumanizing Institutions: Slavery and Contemporary Minority Social Control in the U.S. Northeast and South
Authors: Ward Matthew 
Published: March 2022

Abstract
Scholarship on slavery's legacy and enduring consequences has largely been limited to the South under the assumption that this region continues to be more deeply affected by slavery than other regions, notably the Northeast. Also overlooked is the extent to which slavery's consequences generalize beyond Black disadvantage, harming other racialized ethnic minority groups such as Latinos. Scholarship has paid little attention to the role of the state's institutions of social control—notably, law enforcement—in transmitting slavery’s legacy after its formal abolishment. I address these issues using quantitative techniques assessing the relationship between prior slave dependence and contemporary policing practices in U.S. Southern and Northeastern counties. I argue that as a result of slavery's influence on local legal apparatuses and institutions of social control—in areas in the South and Northeast where slave dependence was greater—law enforcement today is less likely to protect minorities, resulting in higher rates of hate crime underreporting by police. Findings reveal slavery’s nefarious consequences disadvantage Black populations but also spillover to Latinos, particularly in the South. In both regions, contemporary Black population concentration mediates slavery’s relationship with the rate of police underreporting of anti-Black crimes.
Classification and Coercion: The Gendered Punishment of Transgender Women in Immigration Detention
Authors: Vogler Stefan , Rosales Rocío 
Published: March 2022

AbstractAlthough transgender immigrants are a highly vulnerable and growing population, little sociological or criminological work has examined their experiences. This paper begins to fill that gap through in-depth life history interviews with thirteen transgender women migrants in detention and a survey of fifty-five transgender women migrants who experienced detention. Though the detention system allows trans migrants to be classified as such for housing and immigration relief (e.g., asylum), we show that the classification processes that trans women encounter continue to marginalize them and expose them to particularly gendered forms of punishment. We thus argue that adding new categories does little to ameliorate gendered inequalities without a concomitant commitment to shifting organizational cultures of classification. To support these claims, we show that being classified as transgender can serve as a punishment itself, and secondly, that such classification still exposes transgender women to unique forms of gendered violence while in detention. We conclude with implications for the gendered nature of punishment and organizations, suggesting that carceral settings are not only gendered but cisgendered, favoring cis experiences and bodies in ways that disadvantage and punish trans people.
Temporal trend, space risk and factors associated with the occurrence of dengue in northeast Brazil, 2009–2018
Authors: Coutinho Helder Silveira , Silva Jhonathan Oliveira , Santana Gibson Barros de Almeida , do Carmo Rodrigo Feliciano , Souza Sírius Oliveira , de Faria Marcelo Domingues , Matos Thais Silva , da Silva Tarcísio Fulgêncio Alves , Bezerra-Santos Márcio , de Souza Carlos Dornels Freire 
Published: March 2022

Abstract

Background
Dengue is an acute viral disease of major relevance and impact on public health, causing major epidemics around the world, especially in tropical regions. Here we aimed to analyse the temporal trend and spatial risk, as well as social vulnerability factors, associated with the occurrence of dengue in the state of Bahia, Brazil between 2009 and 2018.

Methods
This is an ecological study carried out with all suspected cases of dengue in Bahia between 2009 and 2018. The data were obtained from the National Notifiable Diseases Information System, available on the website of the Health Department of the State of Bahia, and from the Brazilian Institute of Geography and Statistics. We used the Joinpoint regression model, local empirical Bayesian model for smoothing, global and local Moran statistics and spatial scanning statistics. The relationship between the dengue incidence rate and social determinants was tested using Moran's bivariate correlation.

Results
During the study period, 451 847 probable dengue cases were registered in Bahia. A declining trend was observed in 39.28% (n=11) of the state's health regions and 60.71% (n=17) showed a stationary tendency. The spatiotemporal scanning statistic showed nine clusters of dengue occurrence. The largest cluster had a radius of 342.14 km, consisting of 160 municipalities, 120 094 cases (710.20 cases/100 000 inhabitants) and a relative risk of 2.80. In the multivariate regression model, 11 variables showed a significant association: Social Vulnerability Index (SVI), Municipal Human Development Index (MHDI), SVI urban infrastructure, SVI human capital, MHDI longevity, MHDI education, proportion of people living in households with per capita income less than half the minimum wage (in 2010) and who spend more than 1 h commuting, proportion of mothers who are heads of household who did not complete elementary school and with children &lt;15 y of age, activity rate of persons ages 10–14 y and per capita income.

Conclusions
In the analysis of the spatial distribution, areas of risk of disease transmission throughout the state were identified. These results can provide subsidies for the strategic planning of actions, as well as for the implementation of programs and/or public policies in order to control the incidence of dengue in the population.
The Status of SBIRT Training in Health Professions Education: A Cross-Discipline Review and Evaluation of SBIRT Curricula and Educational Research
Authors: McAfee Nicholas W. , Schumacher Julie A. , Madson Michael B. , Villarosa-Hurlocker Margo C. , Williams Daniel C. 
Published: March 2022

Purpose
To assess the quality of curricular research on the Screening Brief Intervention and Referral to Treatment (SBIRT) approach and determine the presence of useful training modalities, particularly motivational interviewing (MI) training, across health care training curricula.

Method
The authors conducted a systematic review of published, peer-reviewed studies in PubMed, ERIC, CINAHL, Ovid HealthSTAR, and PsycINFO databases through March 2021 for English-language studies describing SBIRT, a curriculum for health care trainees, and curricular intervention outcomes. After the records were independently assessed, data were extracted and 20% of the studies were double-coded for interrater reliability.

Results
Of 1,856 studies, 95 were included in the review; 22 had overlapping samples and were consolidated into 10 nested studies, leaving 83 total. Interrater reliability ranged from moderate (κ = .74, P < .001) to strong (κ = .91, P < .001) agreement. SBIRT training was delivered to trainees across many professions, including nursing (n = 34, 41%), medical residency (n = 28, 34%), and social work (n = 24, 29%). Nearly every study described SBIRT training methods (n = 80, 96%), and most reported training in MI (n = 54, 65%). On average, studies reported 4.06 (SD = 1.64) different SBIRT training methods and 3.31 (SD = 1.59) MI training methods. Their mean design score was 1.92 (SD = 0.84) and mean measurement score was 1.89 (SD = 1.05). A minority of studies measured SBIRT/MI skill (n = 23, 28%), and 4 studies (5%) set a priori benchmarks for their curricula.

Conclusions
SBIRT training has been delivered to a wide range of health care trainees and often includes MI. Rigor scores for the studies were generally low due to limited research designs and infrequent use of objective skill measurement. Future work should include predefined training benchmarks and validated skills measurement.
Development and Validation of SCFE Percutaneous Pinning Surgical Simulation
Authors: Ikwuezunma Ijezie A. , Guilbault Ryan , Jain Amit , Lee Rushyuan J. , Varghese Ranjit , Lentz Jonathan M. , Sponseller Paul D. , LaPorte Dawn , Margalit Adam 
Published: March 2022

Background:
In situ screw fixation with a single percutaneously placed femoral screw remains widely accepted for femoral head fixation in adolescent patients with slipped capital femoral epiphysis (SCFE). Given the potential risks involved with this procedure, a simulation whereby surgical skills could be refined before entering the operating room may be of benefit to orthopaedic trainees.

Methods:
We developed a synthetic model for the simulated treatment of SCFE. Five orthopaedic attendings and twenty trainees were recorded performing an in situ percutaneous fixation on the SCFE model. Time, radiation exposure, and final anteroposterior and lateral radiographs of the SCFE model were recorded. After completion, the attendings and trainees answered a Likert-based questionnaire regarding the realism and utility of the simulation, respectively. Two blinded orthopaedic surgeons rated each participant’s skill level based on previously described assessment tools, including a Global Rating Scale (GRS) of technical proficiency and radiographic grading index for screw placement. Performance metrics and survey responses were evaluated for construct validity, face validity, and interrater reliability.

Results:
The attendings demonstrated superior technical proficiency compared with trainees in terms of higher GRS scores (27.9±1.9 vs. 14.7±5.0, P<0.001) and better radiographic grading of screw placement on lateral views (P=0.019). Similarly, compared with the trainees, the orthopaedic attendings demonstrated shorter operative times (11.0±4.1 vs. 14.7±6.2 min, P=0.035) and less radiation exposure (3.7±1.7 vs. 9.5±5.7 mGy, P=0.037). The interrater reliability was excellent for both the GRS scoring (intraclass correlation coefficient=0.973) and radiographic grading (weighted κ=1.000). The attendings and trainees rated the realism and teaching utility of the simulation as “very good,” respectively.

Conclusion:
Our surgical simulation for in situ percutaneous fixation of SCFE represents a valid and reliable measure of technical competency and demonstrates much promise for potential use as a formative educational tool for orthopaedic residency programs.

Level of Evidence:
Level II.
Lutetium-177 Radiopharmeceutical Therapy Extravasation Lessons Learned
Authors: Berry Kendall , Kendrick Jessica 
Published: March 2022

Abstract
Lutetium-177 (177Lu) dotatate has been offered at Fox Chase Cancer Center since 2017 as part of a clinical trial and then in 2018 as a commercially available cancer therapy, and we thought we were prepared for most 177Lu issues by the fall of 2020. A single phone call identified that the Radiation Safety Department had not been prepared to address extravasations. Fortunately for the patient and Radiation Safety, the 177Lu therapies are administered by an infusion nurse and Fox Chase Cancer Center has a robust infusion center. The expertise of our infusion center team helped to quickly identify specific mitigation efforts to employ. A team of radiation safety and diagnostic medical physicists worked together to estimate a tissue dose. Research was also started with the aim of identifying therapeutic 177Lu extravasations as early as possible. The lessons we learned and plans for future early identification of 177Lu dotatate extravasations are the basis of this paper.