bluuebunny/crossref_metadata_2025_split huggingface dataset:
Graphene Biointerface for Cardiac Arrhythmia Diagnosis and Treatment
Authors: Lin Zexu , Kireev Dmitry , Liu Ning , Gupta Shubham , LaPiano Jessica , Obaid Sofian , Chen Zhiyuan , Akinwande Deji , Efimov Igor 
Published: July 2022

Abstract
Heart rhythm disorders, known as arrhythmias, cause significant morbidity and are one of the leading causes of mortality. Cardiac arrhythmias are primarily treated by implantable devices, such as pacemakers and defibrillators, or by ablation therapy guided by electroanatomical mapping. Pharmacological treatments are mostly ineffective. Both implantable and ablation therapies require sophisticated biointerfaces for electrophysiological measurements of electrograms and delivery of therapeutic stimulation or ablation energy. In this work, we report for the first time on graphene biointerface for in vivo cardiac electrophysiology. Leveraging sub-micrometer thick tissue-conformable graphene arrays, we demonstrate sensing and stimulation of the open mammalian heart both in vitro and in vivo. Furthermore, we demonstrate graphene pacemaker treatment of a pharmacologically-induced arrhythmia, AV block. The arrays show effective electrochemical properties, namely interface impedance down to 40 Ohm×cm2, charge storage capacity up to 63.7 mC/cm2, and charge injection capacity up to 704 µC/cm2. Transparency of the graphene structures allows for simultaneous optical mapping of cardiac action potentials and calcium transients while performing electrical measurements and stimulation. Our report presents evidence of the significant potential of graphene biointerfaces for the future clinical device- and catheter-based cardiac arrhythmias therapies.
Knockdown of circHomer1 in the orbitofrontal cortex results in differential encoding of salient stimuli
Authors: Zimmerman Amber , Weick Jason , Papageorgiou Grigorios , Mellios Nikolaos , Brigman Jonathan 
Published: July 2022

Abstract
Identifying genetic factors that influence electrophysiological signatures associated with disordered behavior will reveal mechanisms of cognitive dysfunction relevant to disease. Homer1 is a reproducible gene candidate for synaptic and cognitive function. Alternative splicing produces activity-dependent isoforms including the circular RNA, circHomer1. While dysregulation of circHomer1 expression has been identified across multiple psychiatric and neurodegenerative disorders and is associated with impaired cognitive flexibility, it is unknown whether circHomer1 can induce electrophysiological signatures relevant to cognitive dysfunction in these disorders. Using in vivo microarray recordings within the orbitofrontal cortex (OFC) of awake, behaving mice during a translational touchscreen task, we demonstrate that reduction of circHomer1 within the OFC induces robust changes in multiunit firing rate and local field potential (LFP) coordination and power to salient stimuli. Further, these electrophysiological changes are associated with transcriptional downregulation of glutamatergic signaling effectors and behavioral alterations leading to impaired cognitive flexibility.
Functional Annotation Of Hypothetical Proteins From The Bacillus Paralicheniformis Strain Bac84 Reveals Proteins with Biotechnological Potentials – an In Silico Approach
Authors: Rahman Md Atikur , Heme Uzma Habiba , Parvez Md. Anowar Khasru 
Published: July 2022

Abstract
A significant number of proteins in the genome of the Bacillus paralicheniformis strain Bac84 are annotated as functionally uncharacterized hypothetical proteins. Investigating these proteins' functions may help us to find novel targets for biotechnological applications. Therefore, the purpose of our research was to functionally annotate the hypothetical proteins from its genome. We employed a structured in-silico approach incorporating numerous bioinformatics tools and databases for functional annotation and characterization. Sequences of 414 hypothetical proteins were evaluated and we were able to successfully attribute a function to 37 hypothetical proteins. Moreover, we performed receiver operating characteristic analysis to assess the performance of various tools. Eight proteins were predicted with biotechnological potentials such as coenzyme A biosynthesis, phenylalanine biosynthesis, antibiotic biosynthesis, and others. Evaluation of the performance of the tools showed an accuracy of 98% which represented the rationality of the tools used. This work shows that this annotation strategy will make the functional characterization of unknown proteins easier and can find the target for further investigation.
The role of mechanosensitive TRPV4 channels in the regulation of myometrium spontaneous and oxytocin- induced contractility under normal and pathophysiologically relevant conditions
Authors: Moroz Olesia F. , Drozd Oksana O. , Lavryk Roman T. , Lazorenko Iryna O. , Zholos Alexander V. 
Published: July 2022

Abstract
Pharmacological modulators of TRPV4 activity were examined in the rat and human myometrial tissue as the potential drugs with the perspective of attenuation of premature uterine contractions or for strengthening them in case of their insufficiency. Changes in myometrium contractility under hypotonic stress, which often occurs in patients with preeclampsia, hypertension and diabetes and may lead to the activation of mechanosensitive TRPV4 channels were explored. Using the tissue bath system with force transducers for recording of isometric contraction of isolated human and rat pregnant myometrium strips, the amplitude of contraction, duration of contraction at 50% of maximal peak amplitude (T1/2) and integral force of contractions were measured and analyzed. Pharmacological activation of TRPV4 ion channels by its selective agonist GSK1016790A (0.3 µМ) caused an increase of the tone of human myometrium strips at term that was abolished by the TRPV4 selective antagonist HC067047 (1 µM). Under hypotonic stress the amplitude of force significantly decreased for human strips (p < 0.05) but in rat myometrium it was up to 60% higher (p < 0.05). During oxytocin-stimulated myometrium contractions TRPV4 channels were found to play the role of a limiting factor likely contributing to the reduction of excitability of uterine myocytes. We propose that the effect of activation of TRPV4 channels depends on both certain species-specific features, as well as the functional state of the myometrium. The effect of TRPV4 agonist in the presence of oxytocin opens new avenues to look for similar patterns and interactions in the regulation of uterine contractility.
Circular RNA hsa_circ_0006421 inhibits hepatocellular carcinoma by acting as a ceRNA targeting miR-134-5p/CELF2 pathway
Authors: Deng Yibin , Zhou Lv , Chen Xiaohao , Pu Junxia , Shi Junhao 
Published: July 2022

Abstract
Background: Hepatocellular carcinoma (HCC) has ranked sixth most common cancer in number of malignancies in the worldwide. There is plenty of evidence indicated that circular RNAs (circRNAs) exert vitally important roles in the progression of many malignancies. Nevertheless, the molecular mechanism and role of hsa_circ_0006421 in HCC are still unclear. The aim of our research is to verify the molecular mechanism and effects of hsa_circ_0006421 in HCC.Methods: First, we collected 34 paired HCC tissues and paraneoplastic tissues surgically resected from patients and analyzed the expression of hsa_circ_0006421 in HCC tissues and its relationship with clinicopathological characteristics. Then, CCK8, colony formation, cell migration assay, transwell invasion assay and Annexin-V/PI staining were used to assess the effects of hsa_circ_0006421 on the growth, migration, invasion and apoptosis of HCC cells. Next, quantitative real-time PCR (qRT-PCR) and Western blots were used to detect the expression of miR-134-5p, CELF2 and hsa_circ_0006421. In the end, the targeting interactions of miR-134-5p and hsa_circ_0006421，CELF2 and miR-134-5p were explored by the dual-luciferase reporter assay.Results: Hsa_circ_0006421 was diminished in HCC tissues and its down-regulation was related to cirrhosis history. Knocking down hsa_circ_0006421 promoted HCC cells growth, migration, invasion and inhibited apoptosis, whereas overexpressing hsa_circ_0006421 had the opposite effects. Moreover, hsa_circ_0006421 served as the competing endogenous RNA of miR-134-5p. Subsequently, there was a reciprocal relationship between CELF2 and miR-134-5p. Hsa_circ_0006421 could positively regulate the protein level of CELF2 in HCC. Conclusion: hsa_circ_0006421 inhibits liver cancer by regulating miR-134-5p/CELF2 axis.
“Cuidado, não voa tão perto do sol”: considerações para trabalhar projeto de vida com juventudes brasileiras
Authors: Pessanha Corrêa Giovanna , de Oliveira Euclides Rebeca , Colmenero Cunha Thiago 
Published: July 2022

O Novo Ensino Médio institui o Projeto de Vida como obrigatório no currículo escolar, através de oficinas ou aulas onde educadores e jovens debatem sobre questões de âmbito profissional, social, físico e emocional a partir da reflexão sobre tomada de decisões e resolução de problemas. Trabalhar Projeto de Vida demanda levar em consideração as noções de possibilidades para cada indivíduo, que é singular e atravessado pelo lugar que ocupa em sociedade, e que deve ser analisado pelo docente em sua prática. A partir do alargamento dos conceitos de adolescência, juventudes e produção de subjetividade, apresenta-se a prática da Análise do Vocacional, realizada no projeto de extensão “Construindo Um Processo De Escolhas Mesmo Quando ‘escolher’ Não É Um Verbo Disponível”, vinculado ao Instituto de Psicologia da Universidade Federal do Rio de Janeiro, como uma forma responsável de trabalhar Projeto de Vida, considerando necessariamente a pluralidade de possibilidades interseccionais e potências das juventudes, abarcando raça, classe, gênero, sexualidade, territórios, entendendo que toda vida é feita de múltiplos fatores que precisam ser escutadas pela escola contemporânea - e não tentando adequar diversas formas de ser jovem em projetos de vida irreais ou intangíveis para determinadas classes e formas de ser.
Moveable book untuk menanamkan karakter peduli lingkungan dan minat membaca siswa
Authors: Hidayatuloh Adil , Wangid Muhammad Nur 
Published: July 2022

Penelitian ini bertujuan mengembangkan movable book berbasis pendidikan karakter yang layak dan efektif untuk menanamkan karakter peduli lingkungan dan meningkatkan minat membaca siswa. Pengumpulan data dilakukan melalui observasi, wawancara, dan angket. Validasi produk yang dikembangkan dilakukan oleh ahli bahan ajar, ahli materi, respon siswa, dan guru. Teknik analisis data menggunakan uji statistik dan uji manova. Hasil penelitian berupa movable book berbasis pendidikan karakter dinyatakan valid digunakan berdasarkan validasi ahli dan dan uji coba pada siswa. Kevalidan pada espek bahan ajar memperoleh skor 79 dengan katagori “sangat baik”, aspek materi memperoleh skor 39 dengan kategori “sangat baik”, respon guru memperoleh skor 36 dengan kategori “sangat baik”, dan respon siswa memeperoleh skor 160 dengan kategori “baik”. Movable book yang dikembangkan efektif meningkatkan karakter penduli lingkungan dan minat membaca siswa. Hasil dari uji coba MANOVA pada data uji coba operasional menunjukan taraf signifikansi 0,002 yang berarti 0,05. Hasil ini menunjukkan bahwa terdapat perbedaan signifikan karakter peduli lingkungan dan minat membaca siswa kelas II SD.AbstractThis study aims to develop a movable book based on character education that is feasible and effective to inculcate the characters of environmental care and increase students' reading interest. This research is development research that uses a model from Borg Gall. The data collection is through observation, interviews, and questionnaires. The validation of the developed product is carried out by teaching material experts, material experts, student responses, and teachers. The data analysis used statistical tests and MANOVA test. The result of the study in the form of a movable book based on character education, which is valid based on expert validation and trials on students. The validity of the teaching material specs scored 79 with the "very good" category, the material aspect gave a score of 39, in the "very good" category, the teacher gave a score of 36, in the "very good" category, and the students gave a score of 160, in the "good" category. The developed movable book is effective in improving the students’ character of environmental care and reading interest. The results of MANOVA trials on operational trial data show a significance level of 0.002 which means 0.05. The results show that there are significant differences in the environmental care character and reading interest of grade II students.
EDUCATION FOUNDATION: HISTORY (HISTORY AS THE BASIS OF EDUCATION)
Authors: Riberu Kristianus , Rosvita Laurentia Angela 
Published: July 2022

Pendidikan merupakan pengalaman belajar yang berlangsung dalam lingkungan sepanjang hidup sebagai usaha sadar yang dilakukan oleh keluarga sebagai pusat utama, masyarakat dan pemerintah melalui kegiatan bimbingan baik secara formal maupun nonformal, guna mencapai pribadi yang integral dan utuh. Sejarah telah menjadi salah satu landasan dari pendidikan di Indonesia dan dunia. Perubahan tujuan dan motif pendidikan dari masa ke masa menandakan adanya pengaruh sejarah dalam usaha menjadikan pendidikan dihari esok lebih baik lagi. Tujuan dari penelitian ini adalah mengkaji sejarah pendidikan dunia dan Indonesia serta mengetahui implikasi sejarah terhadap pendidikan. Metode penelitian ini menggunakan studi literatur. Hasil dari penelitian adalah sejarah berimplikasi pada tujuan, proses, maupun inovasi dalam pendidikan.AbstractEducation is an experience that lasts a lifetime of a conscious effort by the family as the primary center, society, and government through tutoring activities both formal and nonformal, in order to achieve an integral and intact person. History has become one of the foundations of education in Indonesia and the world. The changes of course and motives of education overtime suggest historical influences in efforts to make tomorrow's education even better. The purpose of this study is to review the history of Indonesia and the world’s education and to know the historical implications of education. This research method using the literature study method. The result of this study is that history has implications for educational goals, processes, and innovations.
The Challenges Toward Real-world Implementation of Digital Health Design Approaches: Narrative Review
Authors: Duffy Anthony , Christie Gregory J , Moreno Sylvain 
Published: July 2022

Background
Digital health represents an important strategy in the future of health care delivery. Over the past decade, mobile health has accelerated the agency of health care users. Despite prevailing excitement about the potential of digital health, questions remain on efficacy, uptake, usability, and patient outcome. This challenge is confounded by 2 industries, digital and health, which have vastly different approaches to research, design, testing, and implementation. In this regard, there is a need to examine prevailing design approaches, weigh their benefits and challenges toward implementation, and recommend a path forward that synthesizes the needs of this complex stakeholder group.

Objective
In this review, we aimed to study prominent digital health intervention design approaches that mediate the digital health space. In doing so, we sought to examine the origins, perceived benefits, contrasting nuances, challenges, and typical use-case scenarios of each methodology.

Methods
A narrative review of digital health design approaches was performed between September 2020 and April 2021 by referencing keywords such as “digital health design,” “mHealth design,” “e-Health design,” “agile health,” and “agile healthcare.” The studies selected after screening were those that discussed the design and implementation of digital health design approaches. A total of 120 studies were selected for full-text review, of which 62 (51.6%) were selected for inclusion in this review.

Results
A review identifying the 5 overarching digital health design approaches was compiled: user-centered design, person-based design, human-centered design, patient-centered design, and patient-led design. The findings were synthesized in a narrative structure discussing the origins, advantages, disadvantages, challenges, and potential use-case scenarios.

Conclusions
Digital health is experiencing the growing pains of rapid expansion. Currently, numerous design approaches are being implemented to harmonize the needs of a complex stakeholder group. Whether the end user is positioned as a person, patient, or user, the challenge to synthesize the constraints and affordances of both digital design and health care, built equally around user satisfaction and clinical efficacy, remains paramount. Further research that works toward a transdisciplinarity in digital health may help break down friction in this field. Until digital health is viewed as a hybridized industry with unique requirements rather than one with competing interests, the nuances that each design approach posits will be difficult to realize in a real-world context. We encourage the collaboration of digital and health experts within hybrid design teams, through all stages of intervention design, to create a better digital health culture and design ethos.
Efficacy of Group Exercise–Based Cancer Rehabilitation Delivered via Telehealth (TeleCaRe): Protocol for a Randomized Controlled Trial
Authors: Dennett Amy M , Harding Katherine E , Peiris Casey L , Shields Nora , Barton Christian , Lynch Lauren , Parente Phillip , Lim David , Taylor Nicholas F 
Published: July 2022

Background
Access to rehabilitation to support cancer survivors to exercise is poor. Group exercise–based rehabilitation may be delivered remotely, but no trials have currently evaluated their efficacy.

Objective
We aimed to evaluate the efficacy of a group exercise–based cancer rehabilitation program delivered via telehealth compared to usual care for improving the quality of life of cancer survivors.

Methods
A parallel, assessor-blinded, pragmatic randomized controlled trial with embedded cost and qualitative analysis will be completed. In total, 116 cancer survivors will be recruited from a metropolitan health network in Melbourne, Victoria, Australia. The experimental group will attend an 8-week, twice-weekly, 60-minute exercise group session supervised via videoconferencing supplemented by a web-based home exercise program and information portal. The comparison group will receive usual care including standardized exercise advice and written information. Assessments will be completed at weeks 0 (baseline), 9 (post intervention), and 26 (follow-up). The primary outcome will be health-related quality of life measured using the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire at week 9. Secondary measures include walking capacity (6-minute walk test), physical activity (activPAL accelerometer), self-efficacy (Health Action Process Approach Questionnaire), and adverse events. Health service data including hospital length of stay, hospital readmissions, and emergency department presentations will be recorded. Semistructured interviews will be completed within an interpretive description framework to explore the patient experience. The primary outcome will be analyzed using linear mixed effects models. A cost-effectiveness analysis will also be performed.

Results
The trial commenced in April 2022. As of June 2022, we enrolled 14 participants.

Conclusions
This trial will inform the future implementation of cancer rehabilitation by providing important data about efficacy, safety, cost, and patient experience.

Trial Registration
Australian New Zealand Clinical Trials Registry ACTRN12621001417875; https://tinyurl.com/yc5crwtr

International Registered Report Identifier (IRRID)
PRR1-10.2196/38553
COMPUTATIONAL TOOLS ASSISTED FORMULATION OPTIMIZATION OF NEBIVOLOL HYDROCHLORIDE LOADED PLGA NANOPARTICLES BY 32 FACTORIAL DESIGNS
Authors: SEBASTIAN GRACE , PRIYA SNEH , P. JAMES JAINEY , M. M. ABHILASH , SANNIDHI  , PRABHU VINAY KIRAN 
Published: July 2022

Objective: The aim of the present study was to formulate and optimize the PLGA polymeric nanoparticle of Nebivolol Hydrochloride for sustain release of drug
Methods: The drug-excipients interaction was explored by molecular docking studies by in silico tools. The drug-loaded polymeric nanoparticles prepared by emulsion solvent evaporation method using 32 factorial design and characterized for particle size, zeta potential, and entrapment efficiency. Shape and surface morphology was analysed by SEM and TEM. In vitro drug release study was performed by using a diffusion membrane.
Results: The docking analysis inferred that the drug has interacted well with PLGA and PF-68, which could prevent the drug crystal formation. The optimized polymeric nanoparticles had a particle size of 291 nm and entrapment efficiency of 83.4% and were found to be within 95% of CI of the predicted value, which is acceptable. SEM and TEM studies showed that the formed polymeric nanoparticles were smooth, spherical in shape and uniform in size. In vitro drug release study of optimized formulation showed sustained release for prolonged time period
Conclusion: Based on the computational studies and in vitro release studies, the developed Nebivolol hydrochloride loaded in PLGA nanoparticles could be a promising formulation in oral drug delivery for the treatment of hypertension.
A Survey of Dentist Anesthesiologists on Preoperative Intramuscular Sedation
Authors: Guthrie David B. , Epstein Ralph H. , Boorin Martin R. , Sisti Andrew R. , Romeiser Jamie L. , Bennett-Guerrero Elliott 
Published: July 2022

Objective:
The induction of general anesthesia for children and patients with special needs frequently requires preinduction sedation, especially when anxiety and agitation lead to violent or combative behavior. In these situations, preoperative intramuscular (IM) sedation may facilitate patient transfer, intravenous cannulation, and/or mask induction. This survey aimed to capture data regarding the current preoperative IM sedation practices of dentist anesthesiologists.

Methods:
An electronic survey was distributed in 2020 to all members of the American Society of Dentist Anesthesiologists regarding the administration of preoperative IM sedation. It included questions about the demographics of respondents and their patients who require IM sedation, the most common drug regimens used, decision-making criteria regarding ketamine dosing, the intended level of sedation, sequence of anesthetic management following IM sedation, and observed outcomes.

Results:
A total of 193 responses (43%) were received; of those, 162 reported using preoperative IM sedation. Ketamine was included in 98.7% of reported IM drug regimens. The most common IM sedation regimen was combined ketamine and midazolam (median 2.5 mg/kg and 0.1 mg/kg, respectively). Of the respondents who use preoperative IM sedation, 87% reported using the same drug regimen in at least 80% of cases.

Conclusion:
The most frequently reported drug regimen used by dentist anesthesiologists in North America for preoperative IM sedation was a combination of ketamine and midazolam.