bluuebunny/crossref_metadata_2025_split huggingface dataset:
The flourishing scholarship of psychosocial oncology viewed across 30 years through the lens of this journal, Psycho‐Oncology
Authors: Kissane David W. 
Published: April 2022

AbstractObjectiveThis issue celebrates the 30th anniversary of Psycho‐Oncology as a journal, a home for scholarship integrating the psychological, social and behavioural dimensions of cancer. Psycho‐oncology developed as an evidence‐based discipline to offer an alternative to unproven therapies and to optimise coping and support for patients with cancer and their families.MethodsA review of key articles was undertaken for this editorial.ResultsEmpirical studies have defined the prevalence of morbidity arising from cancer and the acute, longer‐term and late effects of its varied treatments. Interventions have been adapted to respond to existential challenges, death anxiety, fear of recurrence, disease progression, palliative care and have been continued into bereavement. Mixed modality treatments have combined psychotropic and psychotherapeutic interventions. Survivorship studies have examined rehabilitation, fertility, sexuality, body image and relational impacts of illness.ConclusionThis journal has helped the discipline to flourish by publishing the innovative outputs of its community of researchers.
FOXO4 interacts with p53 TAD and CRD and inhibits its binding to DNA
Authors: Mandal Raju , Kohoutova Klara , Petrvalska Olivia , Horvath Matej , Srb Pavel , Veverka Vaclav , Obsilova Veronika , Obsil Tomas 
Published: April 2022

AbstractTranscription factor p53 protects cells against tumorigenesis when subjected to various cellular stresses. Under these conditions, p53 interacts with transcription factor Forkhead box O (FOXO) 4, thereby inducing cellular senescence by upregulating the transcription of senescence‐associated protein p21. However, the structural details of this interaction remain unclear. Here, we characterize the interaction between p53 and FOXO4 by NMR, chemical cross‐linking, and analytical ultracentrifugation. Our results reveal that the interaction between p53 TAD and the FOXO4 Forkhead domain is essential for the overall stability of the p53:FOXO4 complex. Furthermore, contacts involving the N‐terminal segment of FOXO4, the C‐terminal negative regulatory domain of p53 and the DNA‐binding domains of both proteins stabilize the complex, whose formation blocks p53 binding to DNA but without affecting the DNA‐binding properties of FOXO4. Therefore, our structural findings may help to understand the intertwined functions of p53 and FOXO4 in cellular homeostasis, longevity, and stress response.
In vivo liquid–liquid phase separation protects amyloidogenic and aggregation‐prone peptides during overexpression in Escherichia coli
Authors: Gabryelczyk Bartosz , Alag Reema , Philips Margaret , Low Kimberly , Venkatraman Anandalakshmi , Kannaian Bhuvaneswari , Shi Xiangyan , Linder Markus , Pervushin Konstantin , Miserez Ali 
Published: April 2022

AbstractStudying pathogenic effects of amyloids requires homogeneous amyloidogenic peptide samples. Recombinant production of these peptides is challenging due to their susceptibility to aggregation and chemical modifications. Thus, chemical synthesis is primarily used to produce amyloidogenic peptides suitable for high‐resolution structural studies. Here, we exploited the shielded environment of protein condensates formed via liquid–liquid phase separation (LLPS) as a protective mechanism against premature aggregation. We designed a fusion protein tag undergoing LLPS in Escherichia coli and linked it to highly amyloidogenic peptides, including β amyloids. We find that the fusion proteins form membraneless organelles during overexpression and remain fluidic‐like. We also developed a facile purification method of functional Aβ peptides free of chromatography steps. The strategy exploiting LLPS can be applied to other amyloidogenic, hydrophobic, and repetitive peptides that are otherwise difficult to produce.
Multivalent Angiomotin‐like 1 and Yes‐associated protein form a dynamic complex
Authors: Vogel Amber , Crawford Alexandra , Nyarko Afua 
Published: April 2022

AbstractMultivalent complexes formed between the cancer‐promoting transcriptional co‐activator, Yes‐associated protein (YAP), and proteins containing short linear motifs of type PPxY modulate cell proliferation and are attractive therapeutic targets. However, challenges producing PPxY polypeptides containing the full binding domain has limited understanding of the assembly process. Here, we successfully produced a polypeptide containing the complete set of three PPxY binding sites of Angiomotin‐like 1 (AMOTL1), a scaffolding protein that regulates the nucleo‐cytoplasmic shuttling of YAP via WW‐PPxY interactions. Using an array of biophysical techniques including isothermal titration calorimetry, size‐exclusion chromatography coupled to multi‐angle light scattering, and solution nuclear magnetic resonance spectroscopy, we show that the AMOTL1 polypeptide is partially disordered, and binds the YAP WW domains to form an ensemble of complexes of varying stabilities. The binding process is initiated by the binding of one YAP WW domain to one AMOTL1 PPxY motif and is completed by transient interactions of the second YAP WW domain with a second AMOTL1 PPxY motif to form an equilibrating mixture composed of various species having two YAP sites bound to two conjugate AMOTL1 sites. We rationalize that the transient interactions fine‐tune the stability of the complex for rapid assembly and disassembly in response to changes in the local cellular environment.
Partitioning the Hill coefficient into contributions from ligand‐promoted conformational changes and subunit heterogeneity
Authors: Roy Mousam , Horovitz Amnon 
Published: April 2022

AbstractHeterooligomers that undergo ligand‐promoted conformational changes are ubiquitous in nature and involved in many essential processes. Conformational switching often leads to positive cooperativity in ligand binding that is reflected in a Hill coefficient with a value greater than one. The subunits comprising heterooligomers can differ, however, in their affinity for the ligand. Such so‐called site heterogeneity results in apparent negative cooperativity that is reflected by a Hill coefficient with a value less than one. Consequently, positive cooperativity due to the ligand‐promoted allosteric switch can be masked, in cases of such heterooligomers, by apparent negative cooperativity owing to site heterogeneity. Here, we derived expressions for the Hill coefficient, in the case of a heterodimer, in which the contributions from the ligand‐promoted allosteric switch and site heterogeneity are separated. Using these equations and simulations for higher order oligomers, we show under which conditions site heterogeneity can significantly mask the extent of observed positive cooperativity.
AustralianBryobiamites (Trombidiformes: Tetranychidae) form a complex of cryptic taxa with unique climatic niches and insecticide responses
Authors: Umina Paul A , Weeks Andrew R , Maino James L , Hoffmann Ary A , Song Sue Vern , Thia Joshua , Severtson Dustin , Cheng Xuan , van Rooyen Anthony , Arthur Aston A 
Published: April 2022

AbstractBACKGROUNDBryobia(Koch) mites belong to the economically important spider mite family, the Tetranychidae, with >130 species described worldwide. Due to taxonomic difficulties and most species being asexual, species identification relies heavily on genetic markers. Multiple putativeBryobiamite species have been identified attacking pastures and grain crops in Australia. In this study, we collected 79 field populations ofBryobiamites and combined these with 134 populations that were collected previously. We characterised taxonomic variation of mites using 28S rDNA amplicon‐based DNA metabarcoding using next‐generation sequencing approaches and direct Sanger sequencing. We then undertook species distribution modelling of the main genetic lineages and examined the chemical responses of multiple field populations.RESULTSWe identified 47 unique haplotypes across all mites sampled that grouped into four distinct genetic lineages. These lineages have different distributions, with three of the four putative lineages showing different climatic envelopes, as inferred from species distribution modelling.Bryobiamite populations also showed different responses to a widely used insecticide (the organophosphate, omethoate), but not to another chemical (the pyrethroid, bifenthrin) when examined using laboratory bioassays.CONCLUSIONOur findings indicate that cryptic diversity is likely to complicate the formulation of management strategies forBryobiamites. Although focussed on Australia, this study demonstrates the challenges of studyingBryobiaand highlights the importance of further research into this complex group of mites across the world. © 2022 The Authors.Pest Management Sciencepublished by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Novel insecticidal 1,6‐dihydro‐6‐iminopyridazine derivatives as competitive antagonists of insect RDL GABA receptors
Authors: Liu Genyan , Zhou Congwei , Zhang Zhisong , Wang Chenchen , Luo Xiaogang , Ju Xiulian , Zhao Chunqing , Ozoe Yoshihisa 
Published: April 2022

AbstractBACKGROUNDThe ionotropic γ‐aminobutyric acid (GABA) receptor (iGABAR) is an important target for insecticides and parasiticides. Our previous studies showed that competitive antagonists (CAs) of insect iGABARs have the potential to be used for developing novel insecticides and that the structural modification of gabazine (a representative CA of mammalian iGABARs) could lead to the identification of novel CAs of insect iGABARs.RESULTSIn the present study, a novel series of 1,3‐di‐ and 1,3,5‐trisubstituted 1,6‐dihydro‐6‐iminopyridazines (DIPs) was designed using a versatile strategy and synthesized using facile methods. Electrophysiological studies showed that several target DIPs (30 μM) exhibited excellent antagonistic activities against common cutworm and housefly iGABARs consisting of RDL subunits. The IC50 values of 3‐(4‐methoxyphenyl), 3‐(4‐trifluoromethoxyphenyl), 3‐(4‐biphenylylphenyl), 3‐(2‐naphthyl), 3‐(3,4‐methylenedioxyphenyl), and 3,5‐(4‐methoxyphenyl) analogs ranged from 2.2 to 24.8 μM. Additionally, several 1,3‐disubstituted DIPs, especially 3‐(4‐trifluoromethoxyphenyl) and 3‐(3,4‐methylenedioxyphenyl) analogs, exhibited moderate insecticidal activity against common cutworm larvae, with >60% mortality at a concentration of 100 mg kg−1. Molecular docking studies showed that the oxygen atom on the three‐substituted aromatic ring could form a hydrogen bond with Arg254, which may enhance the activity of these DIPs against housefly iGABARs.CONCLUSIONThis systematic study indicated that the presence of a carboxyl side chain shorter by one methylene than that of gabazine at the 1‐position of the pyridazine ring is effective for maintaining the stable binding of these DIPs in insect iGABARs. Our study provides important information for the design of novel insect iGABAR CAs. © 2022 Society of Chemical Industry.
Preventive replacement policy of a system considering multiple maintenance actions upon a failure
Authors: Jiang Liying , Li Bo , Hu Jiawen 
Published: April 2022

AbstractAge‐based preventive replacement policy has been extensively studied in the literature. Upon an unexpected failure, most studies assume that only one type of maintenance actions can be executed to restore the system back to work. For example, minimal repair or corrective replacement that bring the system to respective as‐bad‐as‐old and as‐good‐as‐new states. Few studies consider both two types of maintenance actions, while predetermine the structure of the maintenance policy. Modern systems are becoming increasingly complex. Besides minimal repair and corrective replacement, imperfect repair can also be carried out to restore a failed system back to work, which brings it to a state between as‐bad‐as‐old and as‐good‐as‐new. This study investigates an optimal age‐based replacement policy, where minimal repair, corrective replacement, and imperfect repair can be carried out upon an unexpected failure with different maintenance effects. We formulate the problem as a semi‐Markov decision process (SMDP), and prove the existence of the stationary optimal policy. The optimal preventive replacement age threshold and the corresponding optimal maintenance action upon each failure are jointly obtained to minimize the long‐term average maintenance cost per time unit. A numerical study is provided to illustrate the effectiveness of our proposed model.
On the confidence intervals of process capability index Cpm based on a progressive type‐II censored sample
Authors: Akdoğan Yunus 
Published: April 2022

AbstractThis paper is concerned with the process capability index in the case of the progressively type‐II censored sample. In particular, the asymptotic distribution of maximum likelihood estimators of the process capability index is derived and it is used to construct the approximate confidence intervals with the delta rule. In addition, five parametric bootstrap confidence intervals for the index are discussed, in contrast to approximate confidence intervals, do not require second derivatives of the likelihood function. A Monte Carlo simulation study is performed to observe the coverage probabilities and the average width of these intervals. Simulation results indicate that the approximate confidence interval is better than the bootstrap based confidence intervals in terms of the coverage probabilities. Two practical examples are presented to illustrate the usability of the method discussed here.
Structural and electronic properties of HfO2 films on Si through H2O2 wet oxidation with improved thermal stability
Authors: Yu Tung‐Yuan , Lin Kun‐Lin , Chen Pin‐Guang , Chou Tung‐Huan 
Published: April 2022

The thermal stability and material properties of HfO2 thin films on Si substrates with and without H2O2 wet chemical oxidation were investigated. The HfO2 samples were deposited through plasma‐enhanced atomic layer deposition and subjected to thermal annealing. They were then examined using X‐ray diffraction, transmission electron microscopy, X‐ray photoelectron spectroscopy, reflection electron energy loss spectroscopy, and conductive atomic force microscopy. For the Si substrate without H2O2 wet chemical oxidation, a native oxide (~1.8 nm) was formed on the substrate before HfO2 deposition. After the annealing process at 600°C, the band gap (Eg) of the HfO2 films increased from 6.0 to 6.2 eV due to the diffusion of Si into HfO2. Furthermore, the conduction and valence band offsets (ΔEc and ΔEv, respectively) between HfO2 and Si changed from 1.02 to 1.42 and 3.86 to 3.66 eV, respectively. After the H2O2 wet oxidation of the Si substrate, a 1.5‐nm chemical oxide was formed instead of a native oxide. The band offset and Eg values of HfO2 were similar before and after 600°C annealing (ΔEv = 3.86 eV, ΔEc = 1.02 eV, and Eg = 6.0 eV), implying the high thermal stability of the HfO2 films. Accordingly, wet oxidation not only prevents diffusion from chemical oxide but also markedly improves the oxide leakage current, which is useful for developing highly efficient and thermally stable HfO2 gate oxides in Si‐based integrated circuit devices.
How large should the next study be? Predictive power and sample size requirements for replication studies
Authors: van Zwet Erik W. , Goodman Steven N. 
Published: April 2022

AbstractWe use information derived from over 40K trials in the Cochrane Collaboration database of systematic reviews (CDSR) to compute the replication probability, or predictive power of an experiment given its observed (two‐sided) ‐value. We find that an exact replication of a marginally significant result with has less than 30% chance of again reaching significance. Moreover, the replication of a result with still has only 50% chance of significance. We also compute the probability that the direction (sign) of the estimated effect is correct, which is closely related to the type S error of Gelman and Tuerlinckx. We find that if an estimated effect has , there is a 93% probability that its sign is correct. If , then that probability is 99%. Finally, we compute the required sample size for a replication study to achieve some specified power conditional on the ‐value of the original study. We find that the replication of a result with requires a sample size more than 16 times larger than the original study to achieve 80% power, while requires at least 3.5 times larger sample size. These findings confirm that failure to replicate the statistical significance of a trial does not necessarily indicate that the original result was a fluke.
Microfluidic Nanoparticles for Drug Delivery
Authors: Liu Yun , Yang Guangze , Hui Yue , Ranaweera Supun , Zhao Chun‐Xia 
Published: April 2022

AbstractNanoparticles (NPs) have attracted tremendous interest in drug delivery in the past decades. Microfluidics offers a promising strategy for making NPs for drug delivery due to its capability in precisely controlling NP properties. The recent success of mRNA vaccines using microfluidics represents a big milestone for microfluidic NPs for pharmaceutical applications, and its rapid scaling up demonstrates the feasibility of using microfluidics for industrial‐scale manufacturing. This article provides a critical review of recent progress in microfluidic NPs for drug delivery. First, the synthesis of organic NPs using microfluidics focusing on typical microfluidic methods and their applications in making popular and clinically relevant NPs, such as liposomes, lipid NPs, and polymer NPs, as well as their synthesis mechanisms are summarized. Then, the microfluidic synthesis of several representative inorganic NPs (e.g., silica, metal, metal oxide, and quantum dots), and hybrid NPs is discussed. Lastly, the applications of microfluidic NPs for various drug delivery applications are presented.
“New Normal” implications for global talent in the wake of economic nationalism and slowdown
Authors: Showkat Saloome , Wani Tahir , Kaur Jaspreet 
Published: April 2022

AbstractAs a result of global economies' protectionist policies, followed by economic nationalism and a downturn, there is a shortage of talent with the requisite skills, knowledge, and abilities to meet the contingencies amid COVID‐19. The recent developments have necessitated multinational businesses to manage their talent in a new international business context. Therefore, the purpose of this paper is to explore the implications of the “New Normal” for global talent which is manifested by economic nationalism and slowdown. This study used an inductive method to qualitative research, conducted in‐depth interviews with 28 top executives from multinational corporations. The implications for the global talent emerged from the current study as skill enhancement based on the emerged business model; training and development pertinent to new responsibilities and activities; adaptability to changes in the macroenvironment; emphasis on employee proactivity, experimentation, and risk‐taking; emphasis on hyperspecialization to take on contract positions; global talent needs to participate in remote learning; global talent must emphasize on mental health issues; and registering in talent exchange programs to fill talent skill shortages.
Reimagining Discourse in the Classroom
Authors: Grifenhagen Jill F. , Barnes Erica M. 
Published: April 2022

AbstractClassroom discourse serves an important role in language, literacy, and other content area learning in early childhood and elementary classrooms. In this article, the authors define discourse as it relates to teaching and learning and overview the background of research on the topic. Then classroom discourse is reconsidered in the current context, including the field's growing understanding of the importance of knowledge for literacy, increasingly diverse student populations, and the rising prevalence of technology for instruction. Two traditional classroom discourse practices, sharing time and small‐group discussions, are revisited with these considerations. Suggestions are presented for how educators may update their approach to facilitating these types of discourse activities.
Anaesthesia management of transvenous implantation of a single‐chamber, rate‐adaptive pacemaker in a donkey with high‐grade second and third degree atrioventricular block
Authors: d'Anselme Olivia , Robel Matthias , Schwarzwald Colin , Ringer Simone Katja 
Published: April 2022

AbstractA 17‐year‐old, domestic, entire, male, miniature donkey with a history of syncope because of sinus bradycardia, high‐grade second degree and occasional third degree atrioventricular block was referred for pacemaker implantation under general anaesthesia. After local anaesthesia with topical EMLA cream and subcutaneous mepivacaine infiltration and following tranquilisation with intravenous acepromazine, a temporary pacing catheter was inserted via introducer catheter through the left jugular vein into the right ventricle's apex. After initiation of internal pacing, medetomidine and butorphanol were administered intravenously, followed by ketamine and diazepam. Maintenance of anaesthesia was with isoflurane combined with medetomidine and lidocaine constant‐rate infusions. Intramuscular morphine and intravenous medetomidine were administered for recovery. The procedure was uneventful with no anaesthesia‐related complications, and manually assisted recovery was smooth. The present report describes the anaesthetic challenges and management of pacemaker implantation under general anaesthesia in a miniature donkey.
Universal water service delivery: Insights on what it takes from Ghana
Authors: Kumasi Tyhra C. , Nyarko Kwabena B. , Antwi‐Agyei Philip 
Published: April 2022

AbstractAttainment of the government of Ghana's ambitious target of providing safe and reliable basic water services to all persons in Ghana by 2025 requires urgent need to unravel localized barriers to achieve universal access and sustain it. Using the Life Cycle Cost Approach, this paper provides a framework in policy planning and estimates the quantum of funds that needs to be injected into construction, operation, and maintenance of water facilities in order to achieve full water coverage in a district. Using participatory approaches, data were collected from Bongo, East Gonja, and Wa districts of Ghana. Data collection was facilitated by Community Water and Sanitation Agency employing the Resources, Infrastructure, Demand, and Access Strategic Planning Tool. Results show that the total cost of achieving full coverage is estimated at US$ 10,342,190, US$ 15,828,421, and US$ 10,111,616 for Bongo, East Gonja, and Wa East, respectively, in the implementation of water coverage and sustainability activities spanning 2017–2025. We suggest that sustainable water services for all requires more than capital inflows. Strong leadership commitment, bolstered institutions, improved monitoring, and evaluation as well as adequate human resources are critical to ensuring that capital investments translate into effective service delivery.
Charged lepton flavor violation associated with heavy quark production in deep inelastic lepton-nucleon scattering via scalar exchange
Authors: Kiyo Yuichiro , Takeuchi Michihisa , Uesaka Yuichi , Yamanaka Masato 
Published: April 2022

Abstract
We study charged lepton flavor violation (CLFV) associated with heavy quark pair production in lepton-nucleon deep-inelastic scattering ℓiN → ℓjq$$ \overline{q} $$

q
¯

X. Here ℓi and ℓj denote the initial and final leptons; N and X are respectively the initial nucleon and arbitrary final hadronic system. We employ a model Lagrangian in which a scalar and pseudoscalar mediator generates the CLFV. We derive heavy quark structure functions for scalar and pseudoscalar currents and compute momentum distributions of the final lepton for the process. Our focus is on the heavy quark mass effects in the final lepton momentum distribution. We clarify the necessity of inclusion of the heavy quark mass to obtain reliable theory predictions for the CLFV signal searches in the deep-inelastic scattering.