bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of PSMA-PET on Oncologic Control in Prostate Cancer Patients Who Experienced PSA Persistence or Recurrence
Authors: Bianchi Lorenzo , Ceci Francesco , Costa Francesco , Balestrazzi Eleonora , Droghetti Matteo , Piazza Pietro , Pissavini Alessandro , Mei Riccardo , Farolfi Andrea , Castellucci Paolo , Puliatti Stefano , larcher alessandro , Gandaglia Giorgio , Mottrie Alexandre , Briganti Alberto , MORGANTI Alessio G. , Fanti Stefano , Montorsi Francesco , Schiavina Riccardo , Brunocilla Eugenio 
Published: October 2022

Abstract
Background: Prostate Specific Membrane Antigen-Positron Emission Tomography (PSMA-PET) is currently recommended to restage prostate cancer (PCa) and to guide the delivery of salvage treatments. We aim to evaluate the oncologic outcomes of patients with recurrent PCa who received PSMA-PET.
Methods: 324 hormone-sensitive PCa with PSA relapse after radical prostatectomy who underwent PSMA-PET in 3 high-volume European Centres. Patients have been stratified as pre-salvage setting who never received salvage treatments (n=134) and post-salvage setting, including patients who received previous salvage therapies (n=190). Patients with oligorecurrent (≤ 3 lesions) PSMA-positive disease underwent PSMA-directed treatments: salvage radiotherapy (sRT) or Metastases-directed therapy (MDT). Patients with polirecurrent (>3 lesions) PSMA-positive disease were treated with systemic therapy. Patients with negative PSMA-PET were treated with sRT or systemic therapies or observation according to the treating physician preferences. The primary outcome of the study was Progression-free survival (PFS). Secondary outcomes were: Metastases-free survival (MFS) and Castration Resistant Pca free survival (CRPC-FS). Kaplan-Meier analyses assessed PFS, MFS and CRPC-FS in the pre-salvage and post-salvage setting. Multivariable Cox regression models identified predictors of progression and metastatic disease.
Results: Median follow up was 23 months (IQR: 10-34 months). In pre-salvage setting, the PFS, MFS and CRPC-FS estimates at 3 years were 66.2% vs. 38.9%, 95.2% vs 73.7% and 94.9% vs 93.1% in patients with negative vs. positive PSMA-PET, respectively (all p≥0.2). In post-salvage setting, the PFS, MFS and CRPC-FS estimates at 3 years were 59.5% vs. 29.1%, 92.7% vs. 65.1% and 98.8% vs 88.8% in patients with negative vs. positive PSMA-PET, respectively (all p≤0.01). At multivariable analyses, a positive PSMA-PET was an independent predictor of progression (HR=2.15) and metastatic disease (HR 2.37; all p≤0.03).
Conclusion: PSMA-PET in recurrent PCa detects the site of recurrence guiding salvage treatments and has a prognostic role in patients who received previous salvage treatments.
Distinct Resting-State Functional Connectivity Patterns of Anterior Insula Affected by Smoking in Mild Cognitive Impairment
Authors: Zhang Tianyi , Zeng Qingze , Li Kaicheng , Liu Xiaocao , Fu Yanv , Qiu Tiantian , Huang Peiyu , Luo Xiao , Liu Zhirong , Peng Guoping 
Published: October 2022

Abstract
Background
Smoking is a modifiable risk factor for Alzheimer's disease (AD). The insula plays a key role in both smoking and cognition. However, the smoking effects on insula-related networks in cognitively normal controls (CN) and mild cognitive impairment (MCI) patients remain unknown.
Methods
We recruited 129 CN (85 non-smokers and 44 smokers) and 83 MCI (54 non-smokers and 29 smokers). Each underwent neuropsychological assessment and MRI (structural and resting-state functional). Seed-based functional analyses in anterior and posterior insula were performed to calculate the functional connectivity (FC) with voxels in whole brain. Mixed-effect analyses were performed to explore the interactive effects on smoking and cognitive status. Associations between FCand neuropsychological scales were assessed.
Results
Mixed-effect analyses revealed the FC differences between the right anterior insula (RAI) with the left middle temporal gyrus (LMTG) and that with the right inferior parietal lobule (RIPL) (p < 0.01). The FC of RAI in both LMTG and RIPL sees a significant decrease in MCI smokers (p< 0.01). Furthermore, the FC between RAI and RIPL was associated with processing speed (r = -0.35, p = 0.003) and execution (r = -0.45, p< 0.001)in smokers.
Conclusion
Smoking affects insular FC differently between MCI and CN, and could decrease the insula FC in MCI patients. Our study provides evidence of neural mechanisms between smoking and AD.
Prediction of respiratory complications by quantifying lung contusion volume using chest computed tomography in patients with chest trauma
Authors: Lee Na Hyeon , Kim Seon Hee , Seo Sang-hyup , Kim Byeong-Jun , Lee Chi-Seung , Kim Gil Hwan , Park Sung Jin , Ryu Dong Yeon , Kim Ho Hyun , Lee Sang Bong , Park Chan Ik , Kim Jae Hun , Kim Seon Hyun 
Published: October 2022

Abstract
Pulmonary contusion is an important risk factor for respiratory complications in trauma patients. Hence, we aimed to determine the relationship between pulmonary contusion volume and patient outcomes and the predictability of respiratory complications. We retrospectively included 73 patients with a pulmonary contusion on chest computed tomography (CT) from 800 patients with chest trauma admitted to our facility between January 2019 and January 2020. Chest injury severity was expressed as the ratio of the normal lung volume to total lung volume by quantifying pulmonary contusion volume on chest CT. The cut-off value was 80%. Among 73 patients with pulmonary contusion (77% males, mean age: 45.3 years), 28 patients had pneumonia, and 5 had acute respiratory distress syndrome. The number of patients in the severe risk group with <80% of normal lung volume was 38, among which 23 had pneumonia. In predicting pneumonia, the area under the receiver operating characteristic curves for the ratio of the normal lung volume to total lung volume was 0.85 (95% confidence interval: 0.76–0.95, p=0.008), and the optimal threshold was 70.4%. Quantifying pulmonary contusion volume using initial CT in patients with chest trauma allows for identifying patients at high risk of delayed respiratory complications.
The discovery of multiple active mys-related LTR-retroelements within the Neotominae subfamily of cricetid rodents
Authors: Kass David H , Beatty Sarah , Smith Ashlee , Scott Megan , Shah Dishita , Czaplicki Mary 
Published: October 2022

Abstract
Retrotransposon families in the rodent family Cricetidae have been understudied in contrast to Muridae, both taxa classified within the superfamily Muroidea. Therefore, we pursued advancing our knowledge of the unique mys-1 LTR-retroelement identified in Peromyscus leucopus by utilizing intra-ORF PCR, quantitative dot bots, DNA and protein library screens, the generation of molecular phylogenies, and analyses of orthologous LTR-retroelement loci. This led to the discovery of three additional related families of LTR-retroelements. These include a 2900 bp full-length element of mys-related sequences (mysRS), an 8,000 bp element containing the mys ORF1 sequence (mORF1) with ERV-related sequences downstream in the reverse orientation, as well as an 1800 bp element primarily consisting of mys ORF2 (mORF2) related sequences flanked by LTRs. Our data revealed a very limited number of full-length mys elements among genera of the Neotominae subfamily of cricetid rodents. Additionally, although we estimated roughly 5,000–10,000 genomic copies of the mysRS element among Neotominae, most exist as partial copies. The mORF1 elements are also limited to the genomes of the Neotominae subfamily of cricetid rodents, whereas mORF2 appears limited to the Peromyscus genus. Molecular phylogenies demonstrating concerted evolution, along with an assessment of orthologous loci in Peromyscus for the presence or absence of elements, are consistent with recent activity of these novel LTR-retroelement families. Together with known activity of various families of non-LTR retroelements in Peromyscus species, we propose that retrotransposons have been continually contributing to the dynamics of Peromyscus genomes and are plausibly associated with the evolution of the over 50 identified species.
Anomalous enhancement of charge density wave in kagome superconductor CsV3Sb5 approaching the 2D limit
Authors: Song Boqing , Ying Tianping , Wu Xianxin , Xia Wei , Yin Qiangwei , Zhang Qinghua , Song Yanpeng , Yang Xiaofan , Guo Jiangang , Gu Lin , Chen Xiaolong , Hu Jiangping , Schnyder Andreas , Lei Hechang , Guo Yanfeng , Li Shiyan 
Published: October 2022

Abstract
The two-dimensional (2D) kagome lattice is a versatile platform to explore exotic quantum states. The recently discovered kagome superconductors AV3Sb5 (A= Cs, Rb, K) containing vanadium kagome lattices exhibit a variety of intriguing phenomena, such as a charge density wave (CDW) with time-reversal symmetry breaking and possible unconventional superconductivity. In particular, in bulk samples the CDW displays a three-dimensional character and tends to be suppressed by external or chemical pressure. However, the characteristics and stability of the CDW order in atomically thin flakes approaching the 2D limit remain unexplored. Here, through combined electrical transport, scanning transmission electron microscopy (STEM) and Raman scattering measurements, we report an intertwined-order phase diagram of CsV3Sb5 flakes down to the monolayer. After successfully confirming the stability of the kagome lattice down to at least 4 layers, we observe a non-monotonic evolution of the CDW transition temperature TCDW with a reduction of flake thickness. TCDW first decreases to a minimum value of 72 K at 27 layers and then increases abruptly, reaching a record-high value of 120 K at 5 layers. The superconducting transition temperature (Tc) features an inverse variation with TCDW. Flakes with less than 5 layers, however, become more insulating with decreasing thickness. Raman scattering measurements reveal a weakened electron-phonon coupling with the reduction of sample thickness, suggesting that a crossover from electron-phonon coupling to dominantly electronic interactions could account for the non-monotonic thickness dependence of TCDW. Our work demonstrates the novel effects of dimension reduction and carrier doping on quantum states in thin flakes and provides crucial insights into the complex mechanism of the CDW order in the family of AV3Sb5 kagome metals.
Green immobilization and efficient proliferation of Escherichia coli cells in polyvinyl alcohol hydrogel membranes by unidirectional nanopore dehydration
Authors: Zhong Zhi-Hao , Zhang Yu-Qing 
Published: October 2022

Abstract
Background: The immobilized technology for microbial or cells has the advantages of high microbial activity, high microbial density per unit space, good tolerance, strong shock, load resistance, high processing efficiency, and high reuse rate. It is now widely used in environmental remediation, water quality treatment, biodegradation, food industry, chemical analysis, energy development, medicine and pharmaceuticals, and other fields.
Results: A novel Escherichia coli cell-immobilizing polyvinyl alcohol hydrogel membrane (ECI-PVAHM) was prepared by unidirectional nanopore dehydration (UND) from a 10% polyvinyl alcohol (PVA) aqueous solution containing enhanced green fluorescent protein-labeled E. coli. This bacteria-loaded film has high water stability, flexibility, transparency, and mechanical robustness. Its tensile strength, elongation rate, and swelling rate are in the ranges 0.66–0.90 MPa, 300%–390%, and 330–800%, respectively. The effective bacterial load of ECI-PVAHM is 2.375×109–1010 CFU/g (dry weight), which does not affect the original crystal structure of the PVAHM. This biofilm has a porous network structure with pore sizes between 0.2 and 1.0 μm, and these cells are embedded in the PVAHM network. When the immobilized cells were continuously cultured for 20 days, and the medium was renewed twice daily, their relative proliferation efficiency after 40 cycles could still be maintained at ~91%.
Conclusion: The above results show that the cell division, proliferation ability, and metabolic activity of immobilized E. coli were not affected by the physical barrier of the porous network structure of the hydrogel. This UND-based ECI-PVAHM has potential applications in molecular biology, biopharmaceutical expression and production, bioreactors, and fuel cells.
Composition and Structural Identification of Silicon Particles in Diamond Wire Saw Silicon Powder
Authors: Zhang Tian , Wei Kuixian , Yang Shicong , Ma Wenhui 
Published: October 2022

Abstract

Improving the effective utilization of diamond wire saw silicon powder (DWSSP) waste is beneficial to increasing the production profit of the photovoltaic (PV) industry. It is also important to reduce the emissions of DWSSP to control environmental pollution. However, the surface oxides of silicon particles in DWSSP hinder its effective utilization. In this study, five raw DWSSP materials with different oxygen contents were used to reveal the composition and structure of surface oxides. Initially, the presence of amorphous SiO2 was proved by crystal transformation. Then, the SiO2 content was determined by SiO2-CaO phase diagram analysis, and the SiOx content was determined by using the oxygen conservation rule. The variation trend between the SiO2, SiOx, and Si contents in DWSSP and the O content was determined, according to the SiO2 and SiOx contents. Furthermore, the systematic study of raw DWSSP leads to general formula of the SiO2, SiOx, and Si contents with the total O content can be obtained. The three-layer structure of DWSSP was revealed, the DWSSP consisting of a Si core, an intermediate SiOx layer, and a SiO2 shell. This study can accurately identify the composition and structure of DWSSP and provide a better theoretical basis for the selection and design of the DWSSP recovery process.
An unusual case of huge fibrotic sac of hematoma at saphenous vein harvest site for CABG
Authors: Lee June , Na Hye Rim , Hong Seok Beom , Kim Do Yeon , Kim Hwan Wook , Kim Yong Han 
Published: October 2022

Abstract
Great saphenous vein is a conduit commonly used for coronary artery bypass grafting. However, several complications could occur at leg wound site for vein harvesting. Here, we describe a huge sac of hematoma as an uncommon complication of saphenous vein harvest for coronary artery bypass grafting. A 62-year-old gentleman was readmitted with swelling at left thigh 30 days after coronary artery bypass grafting. Lower extremity computed tomography was suggestive of an oval and thick sac implying a hematoma or seroma. After using ultrasound scanning for the mass, an incision through the previous surgical wound showed a huge mass looking like a ‘sweet potato’. Inspection after incision the mass revealed an old hematoma within the sac. Pathologic findings demonstrated chronic inflammation with the hematoma surrounded by a fibrotic sac. The patient's postoperative course was uneventful without recurrence. Our experience suggests the possibility of a huge hematoma within a thick fibrotic sac at the previous vein harvest site for coronary artery bypass grafting. We hope that this case will contribute to the management of leg wound complications in similar cases.
Association between age and the host response in critically ill patients with sepsis
Authors: Michels Erik H.A. , Butler Joe M. , Reijnders Tom D.Y. , Cremer Olaf L. , Scicluna Brendon P. , Uhel Fabrice , Peters-Sengers Hessel , Schultz Marcus J. , Knight Julian C. , Vught Lonneke A. van , Poll Tom van der 
Published: October 2022

Abstract
Background
The association of ageing with increased sepsis mortality is well established. Nonetheless, current investigations on the influence of age on host response aberrations are largely limited to plasma cytokine levels while neglecting other pathophysiological sepsis domains like endothelial cell activation and function, and coagulation activation. The primary objective of this study was to gain insight into the association of ageing with aberrations in key host response pathways and blood transcriptomes in sepsis.
Methods
We analysed the clinical outcome (n = 1952), 16 plasma biomarkers providing insight in deregulation of specific pathophysiological domains (n = 899), and blood leukocyte transcriptomes (n = 488) of sepsis patients stratified according to age decades. Blood transcriptome results were validated in an independent sepsis cohort and compared with healthy individuals.
Results
Older age was associated with increased mortality independent of comorbidities and disease severity. Ageing was associated with lower endothelial cell activation and dysfunction, and similar inflammation and coagulation activation, despite higher disease severity scores. Blood leukocytes of patients ≥ 70 years, compared to patients < 50 years, showed decreased expression of genes involved in cytokine signaling, and innate and adaptive immunity, and increased expression of genes involved in hemostasis and endothelial cell activation. The diminished expression of gene pathways related to innate immunity and cytokine signaling in subjects ≥ 70 years was sepsis-induced, as healthy subjects ≥ 70 years showed enhanced expression of these pathways compared to healthy individuals < 50 years.
Conclusions
These data demonstrate age-associated differences in the host response to sepsis and suggest that age should be considered in patient selection in future sepsis trials targeting the immune system and/or the endothelial cell response.
miR-212-5p protects the brain against ischemic damage in rats and its function is regulated by the lncfos/miR-212-5p/CASP7 axis
Authors: Li Shenghua , Qu Xiang , Qin Zhenxiu , Gao Jinggui , Li Jinpin , Liu Jing-Li 
Published: October 2022

Abstract
miR-212-5p has been reported to be involved in many biological processes. However, the role of miR-212-5p in ischemic stroke remains unclear. This study aimed to explore the biological role and potential mechanism of miR-212-5p inbrain damage in rats with ischemic stroke by investigating the lncfos/miR-212-5p/CASP7 axis. Rats were subjected to middle cerebral artery occlusion (MCAO) and intracerebroventricular injection of miRNA agomir, miRNA antagomir, shRNA lentiviral vector or negative control. The neurological deficit score, infarct volume and histopathology, neuronal apoptosis, lncfos, miR-212-5p and CASP7 expression in the peri-infarct area were assessed. In this study, we found thatthe expression level of miR-212-5p was significantly downregulated in the peri-infarct area and blood in MCAO rats and in the blood of patients with ischemic stroke. The double luciferase experiment showed that CASP7 was the direct target gene of miR-212-5p and that lncfos was the direct target gene of miR-212-5p. Lateral ventricular injection of miR-212-5p agomir can effectively inhibit apoptosis induced by ischemic brain damage, reduce infarct volume, improve neurological deficit symptoms and downregulate the expression of CASP7 in the peri-infarct area in MCAO rats. Suppressing lncfos with sh-fos can upregulate the expression of miR-212-5p and plays a neuroprotective role in rat MCAO models. We conclude that miR-212-5p plays a neuroprotective role in rat MCAO models and that its function is regulated by the lncfos/miR-212-5p/CASP7 axis.
Iterative 3D modeling of thermal effects in end-pumped continuous-wave Ho3+:YAG lasers
Authors: Rupp Marius , Eichhorn Marc , Kieleck Christelle 
Published: October 2022

Abstract
We present a highly accurate model for end-pumped continuous-wave Ho3+:YAG laser resonators, based on a vectorial beam propagation method (BPM) algorithm. Thermal effects in the laser crystal like thermal lensing and stress birefringence are taken into consideration. The implementation of these effects is based on an iterative method, which updates the temperature and displacement in the crystal at specific roundtrip intervals. An experimental Ho3+:YAG resonator cavity in continuous wave operation is used to validate the model. In particular, a resonator showing higher order modes and beam distortions at higher pump power is set up, which shows a very good agreement between the model and experimental results.
Experimental investigation of steel fibres and polypropylene fibres effect on Fibre reinforced concrete
Authors: antarvedi Bhawani 
Published: October 2022

Abstract
The study is aimed to determine the effect of varying percentage of dosage of steel and polypropylene fibres in fresh concrete properties and find that optimum percentage of steel-polypropylene fibre which can enhance the more compressive strength, flexural strength and also reducing the crack initiation and reduction of shrinkage cracks. The results present that flexural and compressive strength characteristics influenced by steel and polypropylene fibres. Using steel fibres are found to be more effective for the enhancement of flexural strength characteristics and reducing cracks propagation. Two types of steel fibres namely crimped steel fibre and hooked end steel fibre are used separately with polypropylene fibres. The crimped steel fibre is more effective than the hooked end steel fibre. The workability is also affected by the addition of steel fibres. Workability is more affected by the crimped steel fibres than hooked steel fibres. The amounts of fibres in the SPFRC mixes are varied from 0.5–1% of the total volume of concrete. The variation is also carried out in the combination of steel and polypropylene fibres to enhance the properties of SPFRC Mixes. Steel fibre varies from 0.25–0.85% whereas polypropylene fibre varies from 0.15% and 0.25%. After all the tests carried out on the SPFRC samples, it showed the type of steel fibre to be preferred with Optimum Content of Fibre Mix is proposed.
PD-L1 expression is not a predictive factor for recurrence in resected non-small cell lung cancer Sub-heading: PD-L1 is not a predictive factor for recurrence
Authors: Motono Nozomu , Mizoguchi Takaki , Ishikawa Masahito , Iwai Shun , Iijima Yoshihito , Uramoto Hidetaka 
Published: October 2022

Abstract
Purpose: Although targeting programmed death-1 (PD-1) and its ligand, programmed death-ligand 1 (PD-L1), is an established treatment modality for non-small cell lung cancer (NSCLC), the prognostic relevance of PD-L1 expression in NSCLC patients who undergo pulmonary resection is controversial.
Methods: Two hundred thirty-seven NSCLC patients who underwent pulmonary resection were enrolled and the relationship between PD-L1 and various clinicopathological factors, as well as the prognostic relevance of PD-L1, was evaluated.
Results: PD-L1 expression was significantly higher in male patients (p<0.01), lymphatic invasion (p<0.01), vascular invasion (p<0.01), grade 3–4 differentiation (p<0.01), squamous cell carcinoma (p<0.01), and pathological stage >II (p<0.01), but significantly lower in those who were epithelial growth factor receptor (EGFR) mutation-negative (p<0.01). Relapse-free survival was significantly worse in patients with PD-L1 expression (p=0.04). Univariate analysis showed that male sex (p=0.04), carcinoembryonic antigen expression (CEA) (p<0.01), maximum standardized uptake value (p<0.01), lymphatic invasion (p<0.01), vascular invasion (p<0.01), grade 3–4 differentiation (p<0.01), lower lobe disease (p=0.04), PD-L1 expression (p=0.03), and pathological stage (p<0.01) were significant risk factors of recurrence. In multivariate analysis, CEA expression (p=0.01), lymphatic invasion (p=0.04), and pathological stage (p<0.01) were risk factors for recurrence, whereas PD-L1 expression was not a significant factor of recurrence (p=0.62).
Conclusion: PD-L1 expression was not a risk factor of recurrence but tumor progression tended to increase PD-L1 expression.
Trial registration: The Institutional Review Board of Kanazawa Medical University approved the protocol of this retrospective study (approval number: I392), and written informed consent was obtained from all patients