bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural diversity of the SARS-CoV-2 Omicron spike
Authors: Gobeil Sophie M-C. , Henderson Rory , Stalls Victoria , Janowska Katarzyna , Huang Xiao , May Aaron , Speakman Micah , Beaudoin Esther , Manne Kartik , Li Dapeng , Parks Rob , Barr Maggie , Deyton Margaret , Martin Mitchell , Mansouri Katayoun , Edwards Robert J. , Sempowski Gregory D. , Saunders Kevin O. , Wiehe Kevin , Williams Wilton , Korber Bette , Haynes Barton F. , Acharya Priyamvada 
Published: January 2022

AbstractAided by extensive spike protein mutation, the SARS-CoV-2 Omicron variant overtook the previously dominant Delta variant. Spike conformation plays an essential role in SARS-CoV-2 evolution via changes in receptor binding domain (RBD) and neutralizing antibody epitope presentation affecting virus transmissibility and immune evasion. Here, we determine cryo-EM structures of the Omicron and Delta spikes to understand the conformational impacts of mutations in each. The Omicron spike structure revealed an unusually tightly packed RBD organization with long range impacts that were not observed in the Delta spike. Binding and crystallography revealed increased flexibility at the functionally critical fusion peptide site in the Omicron spike. These results reveal a highly evolved Omicron spike architecture with possible impacts on its high levels of immune evasion and transmissibility.
An LTR retrotransposon in the promoter of a PsMYB10.2 gene associated with the regulation of fruit flesh color in Japanese plum
Authors: Fiol Arnau , García Sergio , Dujak Christian , Pacheco Igor , Infante Rodrigo , Aranzana Maria José 
Published: January 2022

ABSTRACTJapanese plums exhibit wide diversity of fruit coloration. The red to black hues are caused by the accumulation of anthocyanins, while their absence results in yellow, orange or green fruits. In Prunus, MYB10 genes are determinants for anthocyanin accumulation. In peach, QTLs for red plant organ traits map in an LG3 region with three MYB10 copies (PpMYB10.1, PpMYB10.2 and PpMYB10.3). In Japanese plum the gene copy number in this region differs with respect to peach, with at least three copies of PsMYB10.1. Polymorphisms in one of these copies correlate with fruit skin color. The objective of this study was to determine a possible role of LG3-PsMYB10 genes in the natural variability of the flesh color trait and to develop a molecular marker for marker-assisted selection (MAS). We explored LG3-PsMYB10 variability, including the analysis of long-range sequences obtained in previous studies through CRISPR-Cas9 enrichment sequencing. We found that the PsMYB10.2 gene was only expressed in red flesh fruits. Its role in promoting anthocyanin biosynthesis was validated by transient overexpression in Japanese plum fruits. The analysis of long-range sequences identified an LTR retrotransposon in the promoter of the expressed PsMYB10.2 gene that explained the trait in 93.1% of the 145 individuals analyzed. We hypothesize that the LTR retrotransposon may promote the PsMYB10.2 expression and activate the anthocyanin biosynthesis pathway. We provide a molecular marker for the red flesh trait which, together with that for skin color, will serve for the early selection of fruit color in breeding programs.
The Third Transmembrane Domain of Synaptophysin Regulates Initial Fusion Pore Dynamics during Ca2+-Triggered Exocytosis in Chromaffin cells
Authors: Hsiao Yu-Tien , Jackson Meyer B. 
Published: January 2022

AbstractSynaptophysin (syp) is a major secretory vesicle protein comprising four transmembrane domains (TMDs) and a large cytoplasmic C-terminus. The C-terminus of syp has been shown to regulate exocytosis, vesicle cycling, and synaptic plasticity, but the roles of its TMDs remain unclear. SNARE TMDs line initial fusion pores, and structural work along with sequence analysis suggest that TMD III of syp may play a similar role. To test this hypothesis, we expressed TMD III tryptophan mutants in chromaffin cells from mice lacking both syp and its homolog synaptogyrin, and used amperometry to evaluate fusion pores. In contrast to SNARE TMDs, tryptophan substitutions in syp TMD III had no effect on the flux through initial fusion pores. However, these mutants increased the fraction of kiss-and-run events and decreased the initial fusion pore lifetime. Thus, syp TMD III does not line the initial fusion pore, but interacts with it to influence its stability and choice of release mode.
A timer gene network is spatially regulated by the terminal system in theDrosophilaembryo
Authors: Clark Erik , Battistara Margherita , Benton Matthew A. 
Published: January 2022

AbstractIn insect embryos, anteroposterior patterning is coordinated by the sequential expression of the “timer” genescaudal,Dichaeteandodd-paired, whose expression dynamics correlate with the mode of segmentation. InDrosophila, the timer genes are expressed broadly across much of the blastoderm, which segments simultaneously, but their expression is delayed in a small “tail” region, just anterior to the hindgut, which segments during germband extension. Specification of the tail and the hindgut depends on the terminal gap genetailless, but beyond this the regulation of the timer genes is poorly understood. We used a combination of multiplexed imaging, mutant analysis, and gene network modelling to resolve the regulation of the timer genes, identifying 11 new regulatory interactions and clarifying the mechanism of posterior terminal patterning. We propose that a dynamic Tailless expression gradient modulates the intrinsic dynamics of a timer gene cross-regulatory module, delineating the tail region and delaying its developmental maturation.
SepN is essential for assembly and gating of septal junctions in Nostoc sp. PCC 7120
Authors: Kieninger Ann-Katrin , Tokarz Piotr , Weiss Gregor L. , Pilhofer Martin , Maldener Iris 
Published: January 2022

SummaryThe multicellular life style of filamentous cyanobacteria like Nostoc sp. PCC7120 relies on a cell-cell communication system involving so called septal junctions. These are multiprotein complexes, which traverse the septal peptidoglycan through nanopores, connecting the neighboring cells and enabling molecule transfer along the filament. The intercellular communication is crucial when different cell types in the filament, vegetative cells and heterocysts, have to exchange metabolites and signaling molecules. Septal junctions of cyanobacteria can even control the molecule exchange by gating. The multiprotein complex consists of three modules: the septum spanning tube, the plug residing within the cytoplasmic membrane at both ends of the tube and a membrane associated cap module, covering the plug/tube modules on the cytoplasmic side of each neighboring cell. Until now, FraD was the only identified protein component of the septal junction protein complexes and localizes to the plug module. Here, we identified SepN as a new component via co-immunoprecipitation using FraD as bait and further demonstrated its essential role in septal junction assembly. Despite normal septal nanopore formation, a mutant in sepN exhibited a highly reduced rate of intercellular communication and was unable to gate the exchange of molecules. Cryo-electron tomography of cryo-focused ion beam thinned sepN-mutant filaments revealed septal junctions lacking the plug module and lateral cap openings. The combination of missing plug but present cap allowed to deduce the importance of the plug module in ensuring the correct cap architecture and, more importantly, in sealing the diffusion area in the closed septal junction state.
Vibration and position tracking control for a flexible Timoshenko robot arm with disturbance rejection mechanism
Authors: Yang Yan , Shi Jun , Liu Zhijie , Liu Shuangyin 
Published: January 2022

Purpose
This paper aims to study the boundary disturbance rejection control design for a flexible Timoshenko robot arm to diminish external disturbances and achieve desired angle tracking, with system vibration and elastic deformation considered.

Design/methodology/approach
This study introduces disturbance observer and disturbance rejection mechanism into the boundary control design for flexible Timoshenko robot arm systems. The uniform bounded stability of controlled systems is proved via Lyapunov analysis without any simplification of the infinite-dimensional system dynamics.

Findings
The proposed boundary disturbance rejection control scheme can effectively suppress vibrations and shear deformations, achieve the required angular positioning and reject external disturbances. Numerical simulations developed by the finite difference method are adapted to demonstrate the validity of the designed controller.

Originality/value
The originality of this study is to design boundary disturbance rejection control to suppress vibrations and shear deformations for the flexible Timoshenko robot arm, thereby improving the performance and control accuracy of the system.
Architectural approaches to housing customization: introducing the Inhabitant-Driven Customization approach and the MyChanges tool
Authors: Eloy Sara , Vermaas Pieter E. 
Published: January 2022

PurposeCustomization is a paradox in architecture, providing necessary modernization for buildings but potentially damaging their architectural integrity. In this paper, the authors introduce the Inhabitant-Driven Customization approach for avoiding this paradox; this approach lets inhabitants design the customization from options created by architects that safeguard architectural rules. As a first implementation of the Inhabitant-Driven Customization approach, the MyChanges tool is presented. The authors assess whether the approach avoids the customization paradox by a qualitative stakeholder evaluation of the MyChanges tool and by a comparison of the Inhabitant-Driven Customization approach with existing approaches to housing customization.Design/methodology/approachMyChanges is a shape grammar-based design tool developed to enable inhabitants of the Álvaro Siza Vieira Malagueira housing complex to customize their houses in accordance with the architectural language of the complex. In this study, the authors qualitatively evaluated MyChanges with architects and other professional stakeholders. MyChanges is used in this paper to assess if the Inhabitant-Driven Customization approach avoids the paradox of customization. The initial reception of MyChanges produced diverging outcomes, suggesting that Inhabitant-Driven Customization is also unable to avoid the customization paradox. For analyzing this possibility further, this paper describes the main existing approaches to housing customization, including the Inhabitant-Driven Customization approach, formulates nine conditions for these approaches, and provides a qualitative comparative assessment of the approaches.FindingsThe customization paradox is demonstrated in the outcomes of the interviews with professional stakeholders on the MyChanges customization tool for the Malagueira housing complex. An argument is given that makes plausible that the Inhabitant-Driven Customization approach avoids the customization paradox by creating a co-design process in which inhabitants and architects alternately shape customization.Originality/valueThe originality of this paper lies in the introduction and discussion of the paradox of customization in housing. The paper identifies the conditions advanced in architecture for assessing housing customization approaches. Additionally, the authors propose a new customization approach and a design tool that to a large extent fulfills those conditions and avoids the customization paradox.
Uncovering the antecedents of trust in social commerce: an application of the non-linear artificial neural network approach
Authors: Alnoor Alhamzah , Al-Abrrow Hadi , Al Halbusi Hussam , Khaw Khai Wah , Chew XinYing , Al-Maatoq Marwa , Alharbi Raed Khamis 
Published: January 2022

PurposeThe internet creates ample opportunities to start a mobile social commerce business. The literature confirms the issue of customer trust for social commerce businesses is a challenge that must be addressed. Hence, this study aims to examine the antecedents of trust in mobile social commerce by applying linear and non-linear relationships based on partial least squares structural equation modeling and artificial neural network model.Design/methodology/approachThis study applied a non-linear artificial neural network approach to provide a further understanding of the determinants of trust in mobile social commerce based on a non-linear and non-compensatory model. Besides, a questionnaire was distributed to 340 social commerce customers in Malaysia.FindingsThe conceptual framework for investigating trust in mobile social commerce has various advantages and contributions to predicting consumer behavior. The results of the study showed there is a positive and significant relationship between social support, presence and unified theory of acceptance and use of technology2 (UTAUT2). In addition, UTAUT2 has fully mediated the relationship between social support, presence and trust in social commerce. Finally, the results concluded the relationship between UTAUT2 and trust in social commerce would be stronger when the diffusion of innovation and innovation resistance is high and low, respectively.Research limitations/implicationsThe current study provides a novel perspective on how customers can trust social m-commerce to provide real solutions to managers of encouraging e-marketing among consumers.Practical implicationsThis paper shows how businesses can develop trust in social m-commerce in Malaysian markets. The findings of this study probably could be extended to other businesses in Asia or other countries. Because trust in social e-commerce has a dynamic role in consumer behavior and intention to purchase.Originality/valueThis study provided a new perspective on mobile social commerce and paid more attention to an investigation of such emerging commerce. The originality of this study is embodied by investigating an integrated model that included different theories that presented new directions of trust in mobile social commerce through social and behavioral determinants.
“A home away from hem”: exploring and assessing hotel staycation as the new normal in the Covid-19 era
Authors: Yan Qi , Shen Huawen , Hu Yunhong 
Published: January 2022

Purpose
Enlightened by the theoretical framework of adapted protection motivation, this study aims to explore and assess the viability and resilience of hospitality consumption in the ongoing Covid-19 era as embodied in the activity of staycation, which is gaining popularity as a rare escape from the hemming in of the pandemic.

Design/methodology/approach
This study collects data from staycation guests in Hong Kong, which at the time was under semi-lockdown imposing compulsory quarantine for inbound visitors. The data was analyzed through structural equation modeling (SEM).

Findings
It is revealed by the study results that staycation experiences in Hong Kong are underpinned by a full mediating effect between place attachment and experience quality is performed by sense of presence, together with consolidation of experience quality and psychological detachment as tenable mediators in the research model. Aside from the utilitarian and hedonic values, hospitality and tourism consumption have been engrained with profound socio-cultural implications congenial to the collective identities, recollection and contemplation of a civilized society, with the Covid-19 era and the foreseeable future expected to be no exception.

Practical implications
The results of this study can serve as reference regarding better planning and development of the staycation product as effective responses against the grave repercussions of the Covid-19 pandemic by hotel and hospitality practitioners and destination marketers and managers at large. In particular, the pandemic has inadvertently availed the opportunity for the destinationalization of the hotel and hospitality sector, with rich implications for industrial consolidations and coordination with destination authorities.

Originality/value
The holistic structural research model derived and empirically examined entails major antecedents and consequences of the experience quality of staycation guests in locked-down Hong Kong, with the incorporation of the variables of place attachment and extended conceptualization of sense of presence accounting for the efficacy factors of the staycation takers in terms of locality and recollection appraisals, respectively. This study enriches theoretical articulations on staycation as the new normal of hospitality consumption in the lingering pandemic era.
A study of growth barriers and mitigation measures for built environment innovative startups: the case of Hong Kong
Authors: Lam Patrick T.I. , Mok Kelvin S.H. 
Published: January 2022

Purpose
This study aims to identify the challenges facing innovative startups in the construction environment, recommending possible self-help measures and society support.

Design/methodology/approach
A comprehensive literature survey informed a questionnaire survey on built environment startups in Hong Kong, followed by a statistical analysis and supplemented by written views of respondents. Validation by experts confirms the survey results.

Findings
Triangulated findings highlight the problems of conservative policies, investors’ preference on short payback periods, price competition, high operation cost and a lack of promotion channels. The firm’s size and its age differentiate its networking and fund-raising capabilities.

Research limitations/implications
While the survey samples cover the spread of startups in Hong Kong’s construction/real estate industries well, the number is still limited because the city is relatively compact. The barriers and solutions may be particularly relevant to the built environment there, but also worth noting elsewhere.

Practical implications
Built environment startups are emerging and their path of development is obscured by industry barriers. While the findings reflect the current situation in Hong Kong, which is a metropolitan city with a vibrant construction market, government policies may present a varying factor in different economies. Conservatism in the construction industry may also be a hindrance, but gradual signs of improvements are seen.

Originality/value
The recommendations provided may help mitigate the problems of startup growth. They also provide insights into the construction “startup eco-system” worth the attention of policy makers and project managers, who may make better use of the innovative technology and services of built environment startups if the difficulties are alleviated.
Expatriate’s food adaptation: when does acculturation elicit social identification vs differentiation?
Authors: Miocevic Dario , Kvasina Antonija , Crnjak-Karanovic Biljana 
Published: January 2022

Purpose
Extant literature informs that expatriates develop a natural inclination towards host country food with increased acculturation. However, this study argues that expatriates might have divergent goals in different domains of food adaptation (private vs public), which eventually sheds new light on their acculturation process. This paper aims to investigate how expatriate's adaptation in private vis-a-vis public domain influences their food consumption behavior.

Design/methodology/approach
This study draws on the optimal distinctiveness theory and domain-specific view of adaptation and tests the food adaptation outcomes of expatriates’ acculturation through social interactions with the local community. A survey was conducted among 120 expatriate newcomers currently living and working in the Middle East.

Findings
The findings show that acculturation positively influences food adaptation in the private (local food consumption), whereas its relationship with food adaptation in the public domain (local restaurant visits) assumes an inverted U-shape. Furthermore, the authors find that the latter relationship is negatively moderated by expatriate’s overall experience and positively moderated by uncertainty avoidance, which also unfolds situations when an expatriate follows social identification vis-à-vis differentiation pattern.

Originality/value
This work goes beyond the traditional focus on acculturation through social identification and provides evidence that differentiation effects must also be accounted for. The authors argue and provide empirical evidence that expatriates’ greater social interactions with the local community result in different food adaptation outcomes when private vs public consumption domains are considered. In addition, by analyzing the moderating effects of expatriate experience and uncertainty avoidance, the authors further provide evidence on when social identification vis-à-vis differentiation prevails.
Investigating combined effect of WOM and eWOM: role of message valence
Authors: R.V. ShabbirHusain , Varshney Sanjeev 
Published: January 2022

Purpose
The purpose of this study was to investigate consumer preference formation in presence of reviews coming from traditional and electronic word of mouth (eWOM) under different conditions of review valence.

Design/methodology/approach
Scenario-based experimental design was used for this study. A total of 172 respondents were randomly assigned across four experimental scenarios and a control group. Across-groups comparisons were conducted using post hoc Dunnett’s test for checking the hypothesis.

Findings
The study revealed that negativity bias impacted consumer judgement formation to such an extent that positive valence from higher credibility source (WOM) is over-powered by negative reviews from lesser trusted source (eWOM). Further, a negative valence review from higher credibility source (WOM) may not be countered even in presence of consensus of positive valence review coming from a higher number of positive eWOM.

Originality/value
This study contributes to existing literature by examining consumer preference behaviour in presence of reviews coming from traditional and eWOM under different conditions of review valence. While earlier studies have examined individual role of WOM and eWOM under differing valence conditions, this paper extends literature by studying combined effect of WOM and eWOM under different review valence.