bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peritumor tertiary lymphoid structures are associated with infiltrating neutrophils and inferior prognosis in hepatocellular carcinoma
Authors: Zhang Tianchen , Lei Xinjun , Jia Weili , Li Jianhui , Nie Ye , Mao Zhenzhen , Wang Yanfang , Tao Kaishan , Song Wenjie 
Published: September 2022

AbstractBackgroundThe positive prediction of prognosis and immunotherapy within tertiary lymphoid structure (TLS) in cancerous tissue has been well demonstrated, including liver cancer. However, the relationship between TLS and prognosis in the peritumoral region of hepatocellular carcinoma (HCC) has received less attention. Few studies on whether TLS, as a typical representative of acquired immune cell groups, is associated with innate immune cells. The aim of this paper was to identify the prognostic role of peritumor TLS in HCC and to simply explore the relationship with neutrophils infiltration.MethodsThis study included cancerous and paracancerous tissue from 170 patients after surgical resection of HCC. TLS was examined and identified by pathological H&E examination, and the impact on prognosis was further classified by determination of total TLS area. Immunohistochemical staining of CD15+ neutrophils was also performed on half of the cases. The obtained results were validated by external public database, as TLS has been widely shown to be tagged with 12 chemokines.ResultsIn peritumoral tissue, the TLS− group had better overall survival (OS) and disease‐free survival (DFS) outcomes compared with the TLS+ group. On the contrary, the intratumor TLS+ group showed better DFS outcomes. When further investigating the relationship between TLS area distribution and DFS, progressively worse prognosis was only found in the peritumor region with increasing TLS density (TLS− vs. TLSL vs. TLSH). In addition, neutrophil infiltration increased in parallel with TLS density in the peritumoral region, which was not observed in the intratumoral region.ConclusionsTLS might have a dual prognostic role in different regions of HCC. The abundance of peritumoral TLS is an independent influence of DFS. The inconsistent correlation between neutrophils and corresponding TLS in different regions may indicate different pathways of immune aggregation and may serve as an explanation for the different prognosis of TLS, which needs to be specifically explored.
Organizational identification as a trigger for personal well‐being: Associations with happiness and stress through job outcomes
Authors: De Giorgio Andrea , Barattucci Massimiliano , Teresi Manuel , Raulli Giovanni , Ballone Chiara , Ramaci Tiziana , Pagliaro Stefano 
Published: September 2022

AbstractRecent literature highlights that well‐being, happiness, as well as personal stress, has become important for guiding public policy in areas that might involve suboptimal behaviour. Positive life outcomes can extend from one field of life (family, work, sport, children, hobbies, etc.) to another, multiplying success, performance, and health. In the present study, we aimed to verify whether the positive relation between organizational identification and employees' experiences then extends also into a positive association with more general well‐being that is unrelated to the work context. For this purpose, we considered two proxies of organizational well‐being: job satisfaction (on the positive side) and turnover intention (on the negative side) as well as the distal associations with happiness. The study involved 305 workers who completed a questionnaire made up of five scales: organization identification, job satisfaction, turnover intention, happiness, and personal stress. We found that organizational identification is positively related to employees' job satisfaction and negatively related to their intention to leave. Afterwards, job satisfaction and turnover intention were related to personal stress and happiness, suggesting a positive effect of organizational identification above and beyond the work context.
Anti‐Neuroinflammatory Activity of New Naturally Occurring Benzylated Hydroxyacetophenone Analogs from the Endophytic Fungus Alternaria sp. J030
Authors: Jia Shujie , Li Juan , Li Jia , Su Xiangdong , Li Xiao‐Nian , Yao Yao , Xue Yongbo 
Published: September 2022

AbstractChemical studies on the culture broth of the endophytic fungus Alternaria sp. J030 led to the identification of three benzylated hydroxyacetophenone derivatives, bauvaroalterins A–C (1–3), and 34 structurally diverse metabolites (4–37). The new structures were elucidated by extensive spectroscopic analyses including UV, IR, 1D and 2D NMR, HR‐ESI‐MS, and further confirmed using single crystal X‐ray diffraction. The in vitro anti‐neuroinflammatory effects of the co‐isolated metabolites were evaluated in lipopolysaccharide (LPS)‐stimulated microglial cells. Compounds 1–3 were shown to significantly reduce LPS‐induced NO production by inhibiting the expression of iNOS, as well as inhibiting LPS‐induced production of the inflammatory factors TNF‐α, IL‐1β and IL‐6. Further studies revealed that 1–3 were capable of down‐regulating the expression of NF‐κB subunits p50 and p65, thereby suppressing the activation of NF‐κB by inhibiting the LPS‐induced phosphorylation of IκB‐α. Together these findings demonstrate that bauvaroalterins A−C (1–3) exert anti‐neuroinflammatory effects via inhibition of the NF‐κB/iNOS signalling pathway in LPS induced BV‐2 cells.
Left ventricular outflow tract calcification: Does the geometry matter?
Authors: Ueyama Hiroki , Block Peter C. 
Published: September 2022

Key Points
The CoreValve Evolut PRO/PRO + adapt to left ventricular outflow tract (LVOT) calcification by eccentric deformation at the level of the inflow while preserving circularity at the waist, leaflet tips, and outflow.
The CoreValve Evolut PRO/PRO + maintain their favorable hemodynamics and sealing in the short term despite the presence of LVOT calcification.
The effect of eccentric inflow deformation in the long term and geometric comparison of self‐expanding valves and balloon‐expandable valves require further investigation.
When not to treat could be the best option
Authors: Musumeci Giuseppe , Civera Stefania 
Published: September 2022

AbstractTreatment strategy of people who experienced an Out of Hospital Cardiac Arrest without a ST‐segment elevation myocardial infarction is still a matter of debate. Recent randomized trials and a meta‐analysis comparing early. Against delayed coronary angiography in these patients did not find any improvement in short‐term survival with the immediate approach strategy. Further studies are ongoing, whose results will help clarify the correct management of these OHCA‐non STEMI patients.
Should doctors replace machines in prehospital electrocardiogram interpretation?
Authors: Seto Arnold H. , Long Vincent J. 
Published: September 2022

Key Points
Prehospital cath lab activation for STEMI reduces total ischemic time but at the cost of frequent false‐positive activations.
In this study, half of the prehospital STEMI ECGs did not result in cath lab activation after cardiologist review, and that this decision was correct 96‐97% of the time.
Health care will benefit from automation in some contexts but primarily when integrated with human decision making.
A man with panda eyes after a fall
Authors: Mahdi Dana , Yosief Lydia Salem , Butt Umar , Cassidy Aaron , Luckiewicz Andrzej , Malik Adnan Ather , Malik Amman , Jain Neel , Ali M. Adam 
Published: September 2022

AbstractMost commonly caused by trauma, basal skull fractures present with a range of clinical signs. These include periorbital ecchymosis, as seen in this case, as well as rhinorrhea, otorrhoea and post‐mastoid ecchymosis. Suspected cases must be managed with appropriate imaging and medical or surgical treatment as indicated.
Engineering the Electronic Structure of Active Centers in Metalloporphyrins to Boost Oxygen Reduction Reaction Activity
Authors: Zhou Yue , Li Zhongqiu , Xia Xinghua 
Published: September 2022

AbstractInspired by the ligand‐promoted high reactivity of the heme active center in cytochrome c oxidase (CcO) toward oxygen reduction reaction (ORR), this work proposes a new strategy for improving the efficiency of metalloporphyrin‐catalyzed ORR. We carried out a comparative study between two graphene/cobalt porphyrin complexes, the rGO/TMPPCo formed by noncovalent π‐π stacking, and the rGO‐Ci/TMPPCo with cobalt porphyrins (TMPPCo) axially coordinated with the 1‐carboimidazole (Ci) ligands on reduced graphene oxide (rGO) surface. The involvement of the axial ligand was found to greatly reduce the ORR overpotential by 120 mV in acidic medium, while hydrodynamic tests revealed an unaltered reaction mechanism. This positive effect of axial ligand has been rationalized by both electronic “push effect” and steric “trans effect”. The results of this work highlight the importance of a rationally engineered electronic structure of active sites in heterogeneous catalysis, providing insights for the fabrication of highly‐efficient ORR electrocatalysts.
Mouse Models for Cytomegalovirus Infections in Newborns and Adults
Authors: Brizić Ilija , Lisnić Berislav , Krstanović Fran , Brune Wolfram , Hengel Hartmut , Jonjić Stipan 
Published: September 2022

AbstractThis article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay. © 2022 Wiley Periodicals LLC.
A Patient‐to‐Model‐to‐Patient (PMP) Cancer Drug Discovery Protocol for Identifying and Validating Therapeutic Agents Targeting Tumor Regulatory Architecture
Authors: Laise Pasquale , Bosker Gideon , Califano Andrea , Alvarez Mariano J. 
Published: September 2022

AbstractThe current Achilles heel of cancer drug discovery is the inability to forge precise and predictive connections among mechanistic drivers of the cancer cell state, therapeutically significant molecular targets, effective drugs, and responsive patient subgroups. Although advances in molecular biology have helped identify molecular markers and stratify patients into molecular subtypes, these associational strategies typically fail to provide a mechanistic rationale to identify cancer vulnerabilities. Recently, integrative systems biology methodologies have been used to reverse engineer cellular networks and identify master regulators (MRs), proteins whose activity is both necessary and sufficient to implement phenotypic states under physiological and pathological conditions, which are organized into highly interconnected regulatory modules called tumor checkpoints. Because of their functional relevance, MRs represent ideal pharmacological targets and biomarkers. Here, we present a six‐step patient‐to‐model‐to‐patient protocol that employs computational and experimental methodologies to reconstruct and interrogate the regulatory logic of human cancer cells for identifying and therapeutically targeting the tumor checkpoint with novel as well as existing pharmacological agents. This protocol systematically identifies, from specific patient tumor samples, the MRs that comprise the tumor checkpoint. Then, it identifies in vitro and in vivo models that, by recapitulating the patient's tumor checkpoint, constitute the appropriate cell lines and xenografts to further elucidate the tissue context–specific drug mechanism of action (MOA) and permit precise, biomarker‐based preclinical validations of drug efficacy. The combination of determination of a drug's context‐specific MOA and precise identification of patients’ tumor checkpoints provides a personalized, mechanism‐based biomarker to enrich prospective clinical trials with patients likely to respond. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
Edge computing network resource allocation based on virtual network embedding
Authors: Zhan Keqiang , Chen Ning , Santhosh Kumar Sripathi Venkata Naga , Kibalya Godfrey , Zhang Peiying , Zhang Hongxia 
Published: September 2022

SummaryIt is expected that the demand for quality of service (QoS) and quality of experience (QoE) in future 6G scenarios will continue to increase, and edge computing (EC) will continue to receive widespread attention. However, the highly dynamic and connectivity complexity of edge computing networks (ECNs) pose severe challenges to its resource allocation issues. In addition, network virtualization (NV) is widely applied to increase the flexibility of network architectures. Based on the above inspirations, we adopt virtual network embedding (VNE) to make decisions on the resource allocation of ECN. Specifically, we deploy a deep reinforcement learning (DRL)‐based multi‐layer policy network, which is applied to extract environmental information, calculate the available resources of edge nodes, and screen candidate edge nodes that satisfy resource allocation conditions. Second, the resources of edge links are allocated according to the shortest path algorithm. Finally, we build a simulation environment to demonstrate the advantages of the proposed policy network through rich experiments.
Drug‐impaired driving and traffic collisions: Study on a cross section of the Italian population
Authors: Odoardi Sara , Biosa Giulia , Mestria Serena , Valentini Valeria , De Giovanni Nadia , Cittadini Francesca , Strano Rossi Sabina 
Published: September 2022

AbstractThe present study focuses on the association between road accidents and the presence of drugs of abuse markers in the biological fluids of the drivers. Biological fluids collected from 1236 drivers involved in road accidents (54 fatal and 1182 non‐fatal crashes) in the Rome area were analyzed for alcohol and psychotropic drugs, as required by judicial authorities. The substance most frequently detected was alcohol (in 19% of non‐fatal and 32% of fatal crashes), followed by cannabinoids (12% of non‐fatal crashes) and cocaine (9% of non‐fatal and 20% of fatal crashes). The results obtained for cocaine and cannabinoids in blood and urine were compared. We observed the absence or low concentrations of the active drug in blood (cocaine was often below 5 ng/ml and THC below 1 ng/ml), whereas urinary concentrations of metabolites were generally high (benzoylecgonine 250–above 5000 ng/ml, THCCOOH 15–270 ng/ml). The risk of being involved in a road accident if cocaine or cannabis markers were present in the urine specimens was evaluated compared to a control population. The odds ratios calculated, being 8.13 for cannabis and 5.32 for cocaine, suggest a strong association between the presence of these drugs in the urine of drivers and traffic accidents, regardless of their presence in blood samples. The present data suggest that the chance of being involved in a road accident is higher than in the control population even if the subject is no longer “under the influence” of cannabis or cocaine at the time of the accident.
High sensitivity of tropical forest birds to deforestation at lower altitudes
Authors: Mills Simon C. , Socolar Jacob B. , Edwards Felicity A. , Parra Edicson , Martínez‐Revelo Diego E. , Ochoa Quintero Jose Manuel , Haugaasen Torbjørn , Freckleton Robert P. , Barlow Jos , Edwards David P. 
Published: September 2022

AbstractHabitat conversion is a major driver of tropical biodiversity loss, but its effects are poorly understood in montane environments. While community‐level responses to habitat loss display strong elevational dependencies, it is unclear whether these arise via elevational turnover in community composition and interspecific differences in sensitivity or elevational variation in environmental conditions and proximity to thermal thresholds. Here we assess the relative importance of inter‐ and intraspecific variation across the elevational gradient by quantifying how 243 forest‐dependent bird species vary in sensitivity to landscape‐scale forest loss across a 3000‐m elevational gradient in the Colombian Andes. We find that species that live at lower elevations are strongly affected by loss of forest in the nearby landscape, while those at higher elevations appear relatively unperturbed, an effect that is independent of phylogeny. Conversely, we find limited evidence of intraspecific elevational gradients in sensitivity, with populations displaying similar sensitivities to forest loss, regardless of where they exist in a species' elevational range. Gradients in biodiversity response to habitat loss thus appear to arise via interspecific gradients in sensitivity rather than proximity to climatically limiting conditions.
The role of riparian vegetation flexibility in a bio‐hydro‐morphodynamic simulation
Authors: Zhu Runye , Tsubaki Ryota 
Published: September 2022

AbstractBio‐hydro‐morphodynamic simulation is an essential tool for analyzing fluvial processes under the influence of riparian vegetation, and has developed over the past decade. However, most bio‐hydro‐morphodynamic models have neglected the flexibility of vegetation. The flow‐induced deformation of flexible vegetation changes the resistance to water flow. Therefore, flexibility affects water flow surrounding vegetation patches, and alters bed shear stress below and surrounding patches. To investigate the effects of vegetation flexibility in a fluvial, bio‐hydro‐morphodynamic simulation, we incorporated a model for predicting flexible vegetation reconfiguration in a bio‐hydro‐morphodynamic model based on Delft3D. We studied effects by considering and neglecting vegetation flexibility within a gravel bed river with alternate bars. The vegetation used in the simulation was Phragmites japonica, a widely existing reedy grass in riparian environments. Our results indicate that vegetation flexibility can impact river hydrodynamics and morphology development processes. When accounting for vegetation flexibility, water depth decreases, averaged Shields stress within a vegetation patch increases, and channel width with sediment transport increases. Erosion and deposition within the channel are additionally reduced. The significance of vegetation flexibility depends on multiple factors (i.e., the stem density growth rate, rigidity, and the river bed material). Given a fast stem density growth rate or a large rigidity, differences between flexible and rigid vegetation cases could be neglected. The difference between flexible and rigid vegetation was maximized when the bed gravel size fell within a certain range. Our results suggest that the impact of vegetation flexibility on river morphology depends on the mobility of river bed material. A very active or inactive bed reduced the importance of vegetation flexibility on river morphology.
High reproducibility of spleen stiffness measurement by vibration‐controlled transient elastography with a spleen‐dedicated module
Authors: Rigamonti Cristina , Cittone Micol Giulia , Manfredi Giulia Francesca , Sorge Andrea , Moia Riccardo , Patriarca Andrea , Donato Maria Francesca , Gaidano Gianluca , Pirisi Mario , Fraquelli Mirella 
Published: September 2022

Abstract
Spleen stiffness measurement (SSM) by vibration‐controlled transient elastography (VCTE) is a noninvasive technique for estimating portal hypertension in patients with chronic liver disease (CLD), with its reproducibility yet to be established and its feasibility still unknown beyond CLD. We have studied 420 participants from two tertiary referral centers for liver diseases (Novara, Milan): 297 patients with CLD (32% with cirrhosis) of different etiology (Group A), 63 Philadelphia‐negative myeloproliferative neoplasms (Group B), and 60 heathy volunteers (Group C). All underwent SSM by VCTE with a spleen‐dedicated module (SSM@100 Hz) and liver stiffness measurement (LSM), blindly performed by 2 different operators. In total, 1680 VCTE examinations for SSM were performed (1000 in Novara, 680 in Milan), with an overall 3.2% failure rate. Median SSM was 26.5 kPa (interquartile range [IQR] 20.0–42.3) in Group A, 26.3 kPa (IQR 22.3–33.6) in Group B, and 16.1 kPa (IQR 14.6–18.7) in Group C. In Group A, the median LSM was 6.8 kPa (IQR 4.9–11.3) in Novara and 8.3 kPa (IQR 7.1–10.8) in Milan, the proportion of patients with cirrhosis being 34% in Novara and 31% in Milan. The Group A interobserver agreement ICC was 0.90 (0.88–0.92), significantly lower in the absence of splenomegaly (ICC 0.87 vs. 0.91) and in absence of cirrhosis (ICC 0.84 vs. 0.90); overweight slightly, but not significantly reduced the interobserveragreement. The intra‐observer agreement ICC ranged from 0.91 to 0.96 for the four operators. The Group B interobserver agreement ICC was 0.90 (0.83–0.94). In conclusion, SSM measured by the new spleen‐dedicated VCTE module is a feasible, reliable, and highly reproducible tool in patients with CLD and hematological disorders, and in healthy volunteers.
Global colorectal cancer research, 2007‐2021: Outputs and funding
Authors: Begum Mursheda , Lewison Grant , Wang Xiang , Dunne Philip D. , Maughan Tim , Sullivan Richard , Lawler Mark 
Published: September 2022

AbstractThe purpose of this study was to provide an evidence base for colorectal cancer research activity that might influence policy, mainly at the national level. Improvements in healthcare delivery have lengthened life expectancy, but within a situation of increased cancer incidence. The disease burden of CRC has risen significantly, particularly in Africa, Asia and Latin America. Research is key to its control and reduction, but few studies have delineated the volume and funding of global research on CRC. We identified research papers in the Web of Science (WoS) from 2007 to 2021, and determined the contributions of the leading countries, the research domains studied, and their sources of funding. We identified 62 716 papers, representing 5.7% of all cancer papers. This percentage was somewhat disproportionate to the disease burden (7.7% in 2015), especially in Eastern Europe. International collaboration increased over the time period in almost all countries except in China. Genetics, surgery and prognosis were the leading research domains. However, research on palliative care and quality‐of‐life in CRC was lacking. In Western Europe, the main funding source was the charity sector, particularly in the UK, but in most other countries government played the leading role, especially in China and the USA. There was little support from industry. Several Asian countries provided minimal contestable funding, which may have reduced the impact of their CRC research. Certain countries must perform more CRC research overall, especially in domains such as screening, palliative care and quality‐of‐life. The private‐non‐profit sector should be an alternative source of support.
The effects of SFAS 123R on earnings management
Authors: Lee SeungWon 
Published: September 2022

AbstractThis study investigates whether SFAS 123R was effective at curtailing firms’ earnings management associated with stock option compensation. Using a difference‐in‐differences (DiD) analysis, I find that firms with high implied option compensation before SFAS 123R significantly reduced earnings management after SFAS 123R. The sub‐period analysis indicates that option compensation after SFAS 123R is negatively related to earnings management, while option compensation before SFAS 123R is positively related to earnings management. Overall, the findings indicate that SFAS 123R effectively diminished earnings management associated with stock option compensation.