bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conditional catheter‐related thrombosis free probability and risk‐adapted choices of catheter for lung cancer
Authors: Wang Yanfeng , Cui Chanjuan , Liu Xin , Deng Lei , Yang Ke , Li Bin , Xue Jie , Xie Junying , Cui Wei 
Published: May 2022

AbstractBackgroundCurrent predictive tools assess catheter‐related thrombosis (CRT) in patients with lung cancer in a static manner at a single time point of catheterization. The subsequent hazard changes over time are unknown. The conditional catheter‐related thrombosis‐free probability (CCFP) can provide dynamic information on continual CRT‐free expectations. This study aimed to assess the CCFP and hazard rates based on risk categories and various venous access devices (VADs).MethodsThis retrospective study reviewed 939 patients with lung cancer with peripherally inserted central venous catheters (PICCs) or central venous catheters (CVCs) identified at the National Clinical Research Center for Cancer between January 1, 2015 and December 31, 2018. The incidence of CRT has also been reported. Patients were stratified into low‐ and high‐risk groups according to multivariate Cox regression analyses. CCFP is defined as the CRT‐free probability given that patients have no CRT for a definite time.ResultsA total of 507 patients with PICCs and 432 patients with CVCs were included in this study. The 3‐month CCFP increased from 74.2% at catheter insertion to 93.6% at 3 months. The hazards of CRT in the first month were highest (16.4%) and slightly thereafter. The high‐risk group initially had a higher (21.4%) but significantly decreased CRT hazard after 2 months (8.3%), whereas the low‐risk group maintained a comparable lower risk hazard of less than 5% after 1 month. In the overall cohort, patients with CVCs had lower CRT probability than those with PICCs (HR, 1.76; 95% CI: 1.28–2.41; p < 0.01). Further analysis demonstrated that compared with PICCs, CVCs provided a CRT‐free benefit in low‐risk patients (p = 0.02) but not in high‐risk patients (p = 0.06).ConclusionsCCFP increased, and the hazards of CRT decreased over time in a risk‐dependent manner in patients with lung cancer. These valuable dynamic data may help optimize risk‐adjusted choices of VADs and risk‐adjusted prophylactic anticoagulation strategies for patients.
Sensitivity, specificity, and tolerability of the BACTrack Skyn compared to other alcohol monitoring approaches among young adults in a field‐based setting
Authors: Ash Garrett I. , Gueorguieva Ralitza , Barnett Nancy P. , Wang Wuyi , Robledo David S. , DeMartini Kelly S. , Pittman Brian , Redeker Nancy S. , O’Malley Stephanie S. , Fucito Lisa M. 
Published: May 2022

AbstractBackgroundThere is a need for novel alcohol biosensors that are accurate, able to detect alcohol concentration close in time to consumption, and feasible and acceptable for many clinical and research applications. We evaluated the field accuracy and tolerability of novel (BACTrack Skyn) and established (Alcohol Monitoring Systems SCRAM CAM) alcohol biosensors.MethodsThe sensor and diary data were collected in a larger study of a biofeedback intervention and compared observationally in the present sub‐study. Participants (high‐risk drinkers, 40% female; median age 21) wore both Skyn and SCRAM CAM sensors for 1–6 days and were instructed to drink as usual. Data from the first cohort of participants (N = 27; 101 person‐days) were used to find threshold values of transdermal alcohol that classified each day as meeting or not meeting defined levels of drinking (heavy, above‐moderate, any). These values were used to develop scoring metrics that were subsequently tested using the second cohort (N = 20; 57 person‐days). Data from both biosensors were compared to mobile diary self‐report to evaluate sensitivity and specificity in relation to a priori standards established in the literature.ResultsSkyn classification rules for Cohort #1 within 3 months of device shipment showed excellent sensitivity for heavy drinking (94%) and exceeded expectations for above‐moderate and any drinking (78% and 69%, respectively), while specificity met expectations (91%). However, classification worsened when Cohort #1 devices ≥3 months from shipment were tested (area under curve for receiver operator characteristic 0.87 vs. 0.79) and the derived classification threshold when applied to Cohort #2 was inadequately specific (70%). Skyn tolerability metrics were excellent and exceeded the SCRAM CAM (p ≤ 0.001).ConclusionsSkyn tolerability was favorable and accuracy rules were internally derivable but did not yield useful scoring metrics going forward across device lots and months of usage.
T‐cell recovery and evidence of persistent immune activation 12 months after severe COVID‐19
Authors: Taeschler Patrick , Adamo Sarah , Deng Yun , Cervia Carlo , Zurbuchen Yves , Chevrier Stéphane , Raeber Miro E. , Hasler Sara , Bächli Esther , Rudiger Alain , Stüssi‐Helbling Melina , Huber Lars C. , Bodenmiller Bernd , Boyman Onur , Nilsson Jakob 
Published: May 2022

AbstractBackgroundT‐cell lymphopenia and functional impairment is a hallmark of severe acute coronavirus disease 2019 (COVID‐19). How T‐cell numbers and function evolve at later timepoints after clinical recovery remains poorly investigated.MethodsWe prospectively enrolled and longitudinally sampled 173 individuals with asymptomatic to critical COVID‐19 and analyzed phenotypic and functional characteristics of T cells using flow cytometry, 40‐parameter mass cytometry, targeted proteomics, and functional assays.ResultsThe extensive T‐cell lymphopenia observed particularly in patients with severe COVID‐19 during acute infection had recovered 6 months after infection, which was accompanied by a normalization of functional T‐cell responses to common viral antigens. We detected persisting CD4+ and CD8+ T‐cell activation up to 12 months after infection, in patients with mild and severe COVID‐19, as measured by increased HLA‐DR and CD38 expression on these cells. Persistent T‐cell activation after COVID‐19 was independent of administration of a COVID‐19 vaccine post‐infection. Furthermore, we identified a subgroup of patients with severe COVID‐19 that presented with persistently low CD8+ T‐cell counts at follow‐up and exhibited a distinct phenotype during acute infection consisting of a dysfunctional T‐cell response and signs of excessive pro‐inflammatory cytokine production.ConclusionOur study suggests that T‐cell numbers and function recover in most patients after COVID‐19. However, we find evidence of persistent T‐cell activation up to 12 months after infection and describe a subgroup of severe COVID‐19 patients with persistently low CD8+ T‐cell counts exhibiting a dysregulated immune response during acute infection.
“I sort of never felt like I should be worried about it or that I could be worried about it’” an interpretative phenomenological analysis of perceived barriers to disclosure by young people with coeliac disease
Authors: Wheeler Miranda , David Annabel L. , Kennedy Juliet , Knight Matthew 
Published: May 2022

AbstractObjectivesThere has been little research in the United Kingdom regarding young people's experiences of disclosure of psychological difficulties relating to coeliac disease (CD) to others, particularly healthcare professionals. This study sought to address this systematically with a focus on the lived experiences of young people with CD. This study aimed to gain insight into how paediatric gastroenterology services could improve the patient experience for those with CD and support the identification of patients who may benefit from further psychological support.DesignThis study used interpretive phenomenological analysis (IPA) of patient accounts.MethodsSeven young people with CD (aged 11–16 years) were recruited from a UK hospital paediatric gastroenterology service. Semi‐structured interviews were carried out and verbatim transcripts were analysed using IPA to explore young people's experiences of CD and why they might feel able or unable to disclose psychological difficulties associated with their condition to clinicians.ResultsThree superordinate themes were interpreted from the data. The first encapsulated experiences of adjusting to the diagnosis within a developmental context, including the role of adults in information provision and the importance of peer support. The second outlined experiences of managing perceived or actual stigma regarding others’ perceptions of the condition and themselves. The third incorporated perceived barriers to disclosure relating to power, safety, and beliefs about the role of medical professionals.ConclusionsFindings highlight the importance of clinicians continually providing developmentally appropriate information to young people and actively breaking down barriers to disclosure through body language and the use of clear questions regarding emotional experiences.
Optimized detection of germ cell neoplasia in situ in contralateral biopsy reduces the risk of second testis cancer
Authors: Rajpert‐De Meyts Ewa , Jørgensen Niels , Petersen Jørgen Holm , Almstrup Kristian , Aksglaede Lise , Lauritsen Jakob , Rørth Mikael , Daugaard Gedske , Skakkebæk Niels E. 
Published: May 2022

AbstractObjectiveTo evaluate whether optimized and standardized diagnostic procedures would improve detection of germ cell neoplasia in situ (GCNIS) in the contralateral testis of patients with testicular germ cell tumour (TGCT) and decrease the rate of metachronous tumours, which in a nationwide Danish study was estimated to be 1.9%.Patients and MethodsThis was a retrospective analysis of outcomes in 655 patients with TGCT who underwent contralateral biopsies (1996–2007) compared with those in 459 non‐biopsied TGCT controls (1984–1988). The biopsies were performed using a standardized procedure with immunohistochemical GCNIS markers and assessed by experienced evaluators. Initial histopathology reports were reviewed, and pathology and survival data were retrieved from national Danish registers. In 604/608 patients diagnosed as GCNIS‐negative (four were lost to follow‐up), the cumulative incidence of metachronous TGCT was estimated in a competing risk setting using the Grey method. All cases of metachronous TGCT were re‐examined using immunohistochemistry.ResultsGerm cell neoplasia in situ was found in 47/655 biopsied patients (7.2%, 95% confidence interval [CI] 5.4–9.5%). During the follow‐up period (median 17.3 years) five of the 604 GCNIS‐negative patients developed a TGCT. In 1/5 false‐negative biopsies, GCNIS was found on histological revision using immunohistochemistry and 2/5 biopsies were inadequate because of too small size. The estimated cumulative incidence rate of second tumour after 20 years of follow‐up was 0.95% (95% CI 0.10%–1.8%) compared with 2.9% (95% CI 1.3%–4.4%) among the non‐biopsied TGCT patients (P = 0.012). The estimates should be viewed with caution due to the small number of patients with metachronous TGCT.ConclusionsOptimized diagnostic procedures improved the detection rate of GCNIS in patients with TGCT and minimized their risk of developing metachronous bilateral cancer. Urologists should be aware of the importance of careful tissue excision (to avoid mechanical compression) and the need of adequate biopsy size. Performing contralateral biopsies is beneficial for patients' care and should be offered as a part of their management.
HDAC5, negatively regulated by miR‐148a‐3p, promotes colon cancer cell migration
Authors: OuYang Chunli , Shu Guang , Liu Jiaxin , Deng Shumin , Lu Pengyan , Li Yimin , Gan Yaqi , Xie Bintao , Liu Junwen , Yin Gang 
Published: May 2022

AbstractHistone deacetylases (HDACs) are involved in many processes including tumor cell growth and proliferation and regulation of gene expression. To clarify the role of class IIa HDACs in the metastasis of colon adenocarcinoma, we used the class IIa HDAC inhibitor TMP269 and found that it effectively inhibited the migration ability of colon adenocarcinoma cells. Next, we silenced the member of class IIa HDACs and confirmed that the migratory ability of colon adenocarcinoma cells was significantly inhibited by silencing HDAC5 or HDAC7. HDAC5 plays a variety of roles in human cancers. Here, we examined the role of HDAC5 in colon adenocarcinoma. The results indicated that HDAC5 was highly expressed in tumor tissues and negatively correlated with the expression of miR‐148a‐3p. Moreover, the expression of HDAC5 was correlated with tumor progression. HDAC5 markedly increased the invasion and migration of cancer cells in vitro, an effect that could be inhibited by overexpression of miR‐148a‐3p. Following an intraperitoneal injection of colon adenocarcinoma cells in athymic nude mice, HDAC5 promoted tumor implant. Together, these findings showed that HDAC5 overexpression in colon adenocarcinoma is consistent with tumor progression and tumor cell migration and the impact of HDAC5 overexpression is reduced by miR‐148a‐3p.
Restorative angle of zirconia restorations cemented on non‐original titanium bases influences the initial marginal bone loss: 5‐year results of a prospective cohort study
Authors: Strauss Franz J. , Siegenthaler Marina , Hämmerle Christoph H. F. , Sailer Irena , Jung Ronald E. , Thoma Daniel S. 
Published: May 2022

AbstractAimTo assess radiographic, restorative, clinical and technical outcomes as well as patient satisfaction of directly veneered zirconia restorations cemented on non‐original titanium bases over 5 years.Material and MethodsTwenty‐four patients with a single missing tooth in the aesthetic zone were recruited. All patients received a two‐piece implant with a screw‐retained veneered zirconia restoration cemented extraorally on a titanium base abutment. Marginal bone levels (MBL), marginal bone changes, technical complications, patient satisfaction and clinical parameters including probing depth, bleeding on probing and plaque index were assessed at crown delivery (baseline), at 1 year (FU‐1) and 5 years (FU‐5) of follow‐up. To investigate the relationship between restorative angle and MBL as well as marginal bone changes (bone loss/bone gain), correlation tests and linear regression models were carried out.ResultsTwenty‐two patients were available for re‐examination at 5 years. The mean MBL amounted to 0.54 ± 0.39 mm at baseline, and to 0.24 ± 0.35 at FU‐5 (=bone gain) (p < .001). At FU‐1, a positive correlation (r = .5) between the mesial restorative angle and mesial MBL was found (p = .012). Marginal bone changes between baseline and FU‐1 at mesial sites were also positively correlated with the mesial restorative angle (r = .5; p = .037). Linear and logistic regression models confirmed that mesial marginal bone loss was significantly associated with the mesial restorative angle at FU‐1 (p < .05). At 5 years, these significant associations at mesial sites disappeared (p > .05). At distal sites, no correlations or associations between the restorative angle and MBL or marginal bone changes were found regardless of the time point. During the 5‐year follow‐up, 5 technical complications occurred, mainly within the first year and mostly chippings. All patients were entirely satisfied with their implant‐supported restoration at 5 years.ConclusionWithin the limitations of the present study, the restorative angle of implant‐supported crowns on non‐original titanium bases might influence the initial marginal bone loss but without affecting their favourable long‐term clinical performance. A restorative angle of <40° may limit the initial marginal bone loss at implant‐supported crowns with titanium bases.
Building a pervasive social gaming experience using SocialPG
Authors: Valera‐Aranguren Ramón , Rodriguez Patricia Paderewski , Vela Francisco Luis Gutierrez , Arango‐López Jeferson , Moreira Fernando 
Published: May 2022

AbstractPervasive computing has become a key element to build applications that use fun as a motivating component because it allows exploring new interaction schemes by making the concept of space and time ambiguous and confusing. The present research describes a pervasive social gaming experience, using as a reference SocialPG, which is a model that describes social expansion as a strategy to improve gaming experiences supported by pervasive computing. In this article a description of the model is offered, software architecture is proposed to support it and a case study related to the process of error resolution and detection of improvement opportunities in software products is developed, finally, a general idea about the final software product that will support the game experience is offered together with an evaluation performed by a set of users, where some important findings are highlighted, such as the importance of the missions as a unit of cooperative work and the spectator's participation.
The genomic and proteomic landscape in oral lichen planus versus oral squamous cell carcinoma: a scoping review
Authors: Xie Fangyi , Meves Alexander , Lehman Julia S. 
Published: May 2022

AbstractBackgroundOral lichen planus (OLP), a World Health Organization (WHO)‐classified oral potentially malignant condition, confers a 1% risk of transformation to oral squamous cell carcinoma (OSCC). There does not appear to be a consensus understanding of the underlying molecular events. This scoping review aimed to identify critical molecular pathways and highlight gaps in existing knowledge on malignant transformation in OLP.MethodsFollowing Preferred Reporting Items for Systematic Reviews and Meta‐Analysis Protocols (PRISMA‐P) guidelines, a comprehensive literature search and methodical screening identified 61 relevant studies detailing molecular differences between OLP and OSCC.ResultsMolecular changes shared between OLP and OSCC included those affecting cellular proliferation (altered p53 expression, hypermethylation of p16/CDKN2A, MYC gains, increased ki‐67), apoptosis (increased bcl‐2 and survivin expression), extracellular matrix (ECM) remodeling (increased matrix metalloproteinase [MMP] expression), and transcriptional control (altered bmi1 and microRNA [miRNA] expression). In addition, some molecular alterations accumulated incrementally from control to OLP to OSCC or were present in higher‐risk erosive variants of OLP or transformed OLP. Few studies included rigorous diagnostic inclusion criteria or unbiased discovery methods.ConclusionsResults of this review support the potentially malignant nature of OLP and imply that molecular events associated with malignant transformation may be heterogeneous. In addition, findings in this review highlight the need for additional studies using rigorous diagnostic inclusion criteria and unbiased discovery methods to further understand this process.
Improvement of the solubility and emulsification of rice protein isolate by the pH shift treatment
Authors: Dai Hongmin , Zhan Fuchao , Chen Yijie , Shen Qian , Geng Fang , Zhang Ziyang , Li Bin 
Published: May 2022

SummaryIn the current research, we have adopted a pH shift treatment method that can significantly improve the solubility and emulsification of rice protein isolate (RPI) under neutral conditions. Our results showed that the RPI reached a quasi‐equilibrium state at 1 h after alkali shift treatment, and the solubility and emulsifying properties of RPI were significantly improved compared with the control. In addition, the particle size of RPI decreased considerably under the alkali shift treatment. The results of electron microscopy showed that the RPI aggregates were depolymerised and the particle size distribution was more uniform. The pH shift treatment induced the rearrangement of protein secondary structure, and the exposure of hydrophobic groups increase the surface hydrophobicity of RPI. The change in endogenous fluorescence spectrum, UV and the disulfide bond content all showed that the conformation of RPI changed significantly through pH shift treatment. In addition, it was found that the RPI treated under pH 10–11 shift possessed the best property to stabilise the emulsion. This study showed that under the condition of pH10 and pH11 shift treatment, the structural and functional properties of RPI were vastly improved, and the application of RPI in food was greatly ameliorated.
Mitonuclear discordance and patterns of reproductive isolation in a complex of simultaneously hermaphroditic species, the Allolobophora chlorotica case study
Authors: Dupont Lise , Audusseau Hélène , Porco David , Butt Kevin R. 
Published: May 2022

AbstractHistorical events of population fragmentation, expansion and admixture over geological time may result in complex patterns of reproductive isolation and may explain why, for some taxa, the study of mitochondrial (mt) and nuclear (nu) genetic data results in discordant evolutionary patterns. Complex patterns of taxonomic diversity were recently revealed in earthworms for which distribution is largely the result of paleogeographical events. Here, we investigated reproductive isolation patterns in a complex of cryptic species of earthworms in which discordant patterns between mt and nu genetic lineages were previously revealed, the Allolobophora chlorotica aggregate. Using four nu microsatellite markers and a fragment of the cytochrome c oxidase subunit I mt gene, we carried out a parentage analysis to investigate the mating patterns (i) between individuals belonging to two divergent mt lineages that cannot be distinguished with nu markers and (ii) between individuals belonging to lineages that are differentiated both at the mt and nu levels. Amongst the 157 field‐collected individuals, 66 adults were used in cross‐breeding experiments to form 22 trios based on their assignment to a mt lineage, and 453 obtained juveniles were genotyped. We showed that adults that mated with both their potential mates in the trio produced significantly more juveniles. In crosses between lineages that diverged exclusively at the mt level, a sex‐specific pattern of reproduction characteristic to each lineage was observed, suggesting a possible conflict of interest concerning the use of male/female function between mating partners. In crosses between lineages that diverged both at the mt and nu levels, a high production of cocoons was counterbalanced by a low hatching rate, suggesting a post‐zygotic reproductive isolation. Different degrees of reproductive isolation, from differential sex allocation to post‐zygotic isolation, were thus revealed. Lineages appear to be at different stages in the speciation process, which likely explain the observed opposite patterns of mitonuclear congruence.