bluuebunny/crossref_metadata_2025_split huggingface dataset:
Different effects of NK cells and NK-derived soluble factors on cell lines derived from primary or metastatic pancreatic cancers
Authors: Fiore Piera Filomena , Di Pace Anna Laura , Conti Libenzio Adrian , Tumino Nicola , Besi Francesca , Scaglione Silvia , Munari Enrico , Moretta Lorenzo , Vacca Paola 
Published: November 2022

AbstractNatural killer (NK) cells are cytotoxic lymphoid cells that play a key role in defenses against tumors. However, their function may be severely impaired in patients with pancreatic adenocarcinoma (PA). Indeed, PA cells release soluble factors, thereby generating an immunosuppressive environment that dysregulates NK-cell cytolytic function and favors tumor immune evasion. Here, we analyzed the interactions between NK and PA cells using the PANC-1 and CAPAN-1 cell lines derived from a ductal PA and metastatic lesion, respectively. Metastatic and nonmetastatic cell lines were both able to impair NK cytolytic activity. An analysis of the effect of NK cells and NK-cell-derived exosomes revealed substantial differences between the two cell lines. Thus, NK cells displayed higher cytotoxicity against nonmetastatic PA cells than metastatic PA cells in both 2D cultures and in a 3D extracellular matrix cell system. In addition, NK-derived exosomes could penetrate only PANC-1 spheroids and induce cell killing. Remarkably, when PANC-1 cells were exposed to NK-derived soluble factors, they displayed substantial changes in the expression of genes involved in epithelial-to-mesenchymal transition (EMT) and acquired resistance to NK-mediated cytolysis. These results, together with their correlation with poor clinical outcomes in PA patients, suggest that the induction of resistance to cytolysis upon exposure to NK-derived soluble factors could reflect the occurrence of EMT in tumor cells. Our data indicate that a deeper investigation of the interaction between NK cells and tumor cells may be crucial for immunotherapy, possibly improving the outcome of PA treatment by targeting critical steps of NK-tumor cell crosstalk.
Horizontal transfer of tRNA genes to mitochondrial plasmids facilitates gene loss from fungal mitochondrial DNA
Authors: Nieuwenhuis Mathijs , Groeneveld Jeroen , Aanen Duur K. 
Published: November 2022

AbstractFungal and plant mitochondria are known to exchange DNA with retroviral plasmids. Transfer of plasmid DNA to the organellar genome is best known and occurs through wholesale insertion of the plasmid. Less well known is the transfer of organellar DNA to plasmids, in particular tRNA genes. Presently, it is unknown whether fungal plasmids can adopt mitochondrial functions such as tRNA production through horizontal gene transfer. In this paper, we studied the exchange of DNA between fungal linear plasmids and fungal mtDNA, mainly focusing on the basidiomycete family Lyophyllaceae. We report at least six independent transfers of complete tRNA genes to fungal plasmids. Furthermore, we discovered two independent cases of loss of a tRNA gene from a fungal mitochondrial genome following transfer of such a gene to a linear mitochondrial plasmid. We propose that loss of a tRNA gene from mtDNA following its transfer to a plasmid creates a mutualistic dependency of the host mtDNA on the plasmid. We also find that tRNA genes transferred to plasmids encode codons that occur at the lowest frequency in the host mitochondrial genomes, possibly due to a higher number of unused transcripts. We discuss the potential consequences of mtDNA transfer to plasmids for both the host mtDNA and the plasmid.
Preparation and characterization of a novel luminescence nano-sillenite (Bi12SiO20) glass ceramic within Bi2O3–ZnO–SiO2 system
Authors: Almasarawi Ola N. , Hamzawy Esmat M. A. , Margha Fatma H. , Souaya Eglal R. , Abdel-Hameed Salwa A. M. 
Published: November 2022

AbstractBismuth silicate with sillenite structure (Bi12SiO20) nanophase was prepared via melt–quenching technique in the Bi2O3–ZnO–SiO2 glass system. The effect of replacement ZnO by Bi2O3 was studied. Their thermal behavior showed the change of glass transition temperature (Tg) from 577 °C in the Bi2O3-free glass to 438 °C in ZnO-free glass. In addition, the crystallization temperatures were not only changed from two to one peak, but also decreased from 927 to 476 °C in the same order. According to the heat treatment regimes, willemite, sillenite, tetragonal Bi2O3, cubic Bi2O3 and traces of ZnO were crystallized with different ratios depending on the change in composition and temperature. Sillenite was enhanced with increase heat treatment temperature and/or Bi2O3 additions. Heat treatment at 650 °C/10 h revealed the best regime, where higher degree of crystallization was achieved. The microstructure at 700 ℃/30 min showed nano-scale oriented parallel rod crystals with hexagonal making at their end, whereas clusters of irregular nano-size crystals was appeared at 650 °C/10 h. Transmission spectra of the glasses in UV–Vis–midIR region were increased with Bi2O3 addition reaching 74% in 100B. Photoluminescence properties of both glasses and their corresponding glass–ceramics showed luminescence nature since the blue and green colors were clearly appeared. Calculation of optical bandgap (Eopt) revealed 3.2–2.19 eV with increasing Bi2O3; these values are located in the semiconducting range. The prepared samples can be utilized in electro-optical instruments, also the high transmission in mid-IR nominate it for IR transmitting windows.
Surgical education in the post-COVID era: an EAES DELPHI-study
Authors: Feenstra Tim M. , Tejedor Patricia , Popa Dorin E. , Francis Nader , Schijven Marlies P. 
Published: November 2022

Abstract
Backgrounds
To date, it is unclear what the educational response to the restrictions on minimally invasive surgery imposed by the COVID-19 pandemic have been, and how MIS-surgeons see the post-pandemic future of surgical education. Using a modified Delphi-methodology, this study aims to assess the effects of COVID on MIS-training and to develop a consensus on the educational response to the pandemic.

Methods
A three-part Delphi study was performed among the membership of the European Association of Endoscopic Surgery (EAES). The first survey aimed to survey participants on the educational response in four educational components: training in the operating room (OR), wet lab and dry lab training, assessment and accreditation, and use of digital resources. The second and third survey aimed to formulate and achieve consensus on statements on, and resources in, response to the pandemic and in post-pandemic MIS surgery.

Results
Over 247 EAES members participated in the three rounds of this Delphi survey. MIS-training decreased by 35.6–55.6%, alternatives were introduced in 14.7–32.2% of respondents, and these alternatives compensated for 32.2–43.2% of missed training. OR-training and assessments were most often affected due to the cancellation of elective cases (80.7%, and 73.8% affected, respectively). Consensus was achieved on 13 statements. Although digital resources were deemed valuable alternatives for OR-training and skills assessments, face-to-face resources were preferred. Videos and hands-on training–wet labs, dry labs, and virtual reality (VR) simulation–were the best appreciated resources.

Conclusions
COVID-19 has severely affected surgical training opportunities for minimally invasive surgery. Face-to-face training remains the preferred training method, although digital and remote training resources are believed to be valuable additions to the training palette. Organizations such as the EAES are encouraged to support surgical educators in implementing these resources. Insights from this Delphi can guide (inter)national governing training bodies and hospitals in shaping surgical resident curricula in post pandemic times.
Interdisziplinäre Weiterbildung für Einsätze im urbanen Umfeld
Authors: Hofer Peter 
Published: November 2022

ZusammenfassungMit den Dynamiken in der Entwicklung urbaner Räume, der Rückkehr kriegerischer Auseinandersetzungen in Europa und einem hochvolatilen Umfeld nimmt auch die Bedeutung der Auseinandersetzung mit Einsätzen in einem urbanen Umfeld weiter zu. Die Bewältigung komplexer Herausforderungen erfordert vielfältige Fähigkeiten, die reibungslose Zusammenarbeit unterschiedlicher Organisationen, einen frühzeitigen Informationsaustausch, geeignete Verfahren zur Komplexitätsreduktion sowie die Fähigkeit zu rascher Datenintegration und Visualisierung. Die Vernetzung von Experten und die Entwicklung eines umfassenden Weiterbildungsangebotes für alle betroffenen Stakeholder sind dabei wirkungsvolle Beiträge zu gesamtstaatlicher Resilienz.
On the Stability of Surface Growth: The Effect of a Compliant Surrounding Medium
Authors: Abeyaratne Rohan , Puntel Eric , Tomassetti Giuseppe 
Published: November 2022

AbstractIn a recent paper Abeyaratne et al. (J. Mech. Phys. Solids 167:104958, 2022) concerning the stability of surface growth of a pre-stressed elastic half-space with surface tension, it was shown that steady growth is never stable, at least not for all wave numbers of the perturbations, when the growing surface is traction-free. On the other hand, steady growth was found to be always stable when growth occurred on a flat frictionless rigid support and the stretch parallel to the growing surface was compressive. The present study is motivated by these somewhat unexpected and contrasting results.In this paper the stability of a pre-compressed neo-Hookean elastic half-space undergoing surface growth under plane strain conditions is studied. The medium outside the growing body resists growth by applying a pressure on the growing surface. At each increment of growth, the incremental change in pressure is assumed to be proportional to the incremental change in normal displacement of the growing surface. It is shown that surface tension stabilizes a homogeneous growth process against small wavelength perturbations while the compliance of the surrounding medium stabilizes it against large wavelength perturbations. Specifically, there is a critical value of stretch,$\lambda _{\mathrm{cr}} \in (0,1)$λcr∈(0,1), such that growth is linearly stable against infinitesimal perturbations ofarbitrarywavelength provided the stretch parallel to the growing surface exceeds$\lambda _{\mathrm{cr}}$λcr. Thisstability threshold,$\lambda _{\mathrm{cr}}$λcr, is a function of the non-dimensional parameter$\sigma \kappa /G^{2}$σκ/G2, which is the ratio between two length-scales$\sigma /G$σ/Gand$G/\kappa $G/κ, where$G$Gis the shear modulus of the elastic body,$\sigma $σis the surface tension, and$\kappa $κis the stiffness of the surrounding compliant medium.It is shown that$(a)$(a)$\lambda _{\mathrm{cr}} \to 1$λcr→1as$\kappa \to 0$κ→0and$(b)$(b)$\lambda _{\mathrm{cr}} \to 0^{+}$λcr→0+as$\kappa \to \infty $κ→∞, thus recovering the results in Abeyaratne et al. (J. Mech. Phys. Solids 167:104958, 2022) pertaining to the respective limiting cases where growth occurs$(a)$(a)on a traction-free surface and$(b)$(b)on a frictionless rigid support. The results are also generalized to include extensional stretches.
Simultaneous Removal of Cationic Crystal Violet and Anionic Reactive Yellow Dyes using eco-friendly Chitosan Functionalized by Talc and Cloisite 30B
Authors: Kandil Heba , Ali Heba 
Published: November 2022

AbstractDesigning adsorbent materials that can effectively remove many types of organic dyes is crucial because of the wide diversity of synthetic dyes found in wastewater. Thus, this study presents the synthesis of economic and eco-friendly adsorbent composites composed of chitosan (Cs), talc (T), and Cloisite 30B clay (C) to remove both cationic crystal violet (CV) and anionic reactive yellow 145 (RY) dyes for the first time. Cs was functionalized with T and subsequently the CsTC1 and CsTC2 composites were prepared by sensitizing the obtained CsT with different weight ratios of Cloisite 30B (C). X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), N2adsorption–desorption isotherm, and zeta potential analysis were employed to characterize the materials. The addition of T to Cs matrix improves the thermal stability, pore size, and pore volume with respect to pure Cs. The effect of C inclusion was examined by measuring the affinity of the prepared composites towards adsorption of cationic CV and anionic RY dyes in comparison to pure Cs and CsT composite. The adsorption results in a single dye solution revealed that the CsTC1 composite is the most effective adsorbent for removal of RY dye with 76.9 mg/g adsorption capacity, whereas the CsTC2 composite exhibited the highest adsorption activity for CV dye (37.03 mg/g). Furthermore, RY and CV co-adsorption on CsTC composites was tested from a mixed dye solution. The adsorption kinetics of RY and CV adsorption followed the pseudo second order model. Langmuir isotherm model described the experimental adsorption data better than the Freundlich, Dubinin–Radushkevich, and Temkin isotherm models, indicating a monolayer sorption process for both dyes. The proposed mechanism for RY and CV adsorption using CsTC composites was investigated.
Resumption in Igbo: Two types of resumptives, complex phi-mismatches, and dynamic deletion domains
Authors: Georgi Doreen , Amaechi Mary 
Published: November 2022

AbstractThis paper investigates the morphosyntax of resumption in Igbo (Benue-Congo). The first part addresses the syntax and argues that Igbo has two types of resumptive pronouns (RPs): (i) RPs that terminate base-generation Ā-dependencies, and (ii) RPs at the bottom of Ā-movement dependencies. While similar splits have been claimed to exist in a few other languages, established with a limited data set, Igbo provides pervasive evidence for the co-existence of both types within the same language: type-(ii) RPs occur inallĀ-movement dependencies, and there is comprehensive evidence from a variety of movement tests, including also cyclicity effects, parasitic gap licensing, and language-specific diagnostics. We pursue a spell-out approach to type-(ii) RPs à la Pesetsky (1998) and Landau (2006), and discuss potential reasons behind their restricted distribution: type-(ii) RPs only surface in PPs, DPs, and &Ps, which are thus not (absolute) islands in Igbo. The second part of the paper deals with the morphological side of resumption, viz., with phi- and (alleged) case mismatches between the RP and its antecedent. The phi-mismatch provides further evidence for two types of RPs in Igbo. Moreover, it is more complex than the mismatches reported so far in the literature since the loss of phi-information depends on the type of antecedent (pro/noun, coordination). This pattern poses a challenge for previous accounts of phi-mismatches with movement-derived RPs that are based on static deletion domains. We propose that the cross-linguistic variation in phi-mismatches can be captured in a partial copy deletion approach along the lines of van Urk (2018) if the amount of structure that is deleted is defined dynamically. This further supports the relevance of dynamic domains in morphosyntax, in particular in postsyntactic operations, as previously identified in other areas, e.g., in Moskal’s (2015b) work on contextual allomorphy. Finally, we show that the “case” mismatch is a consequence of the (supra)segmental nature of the relevant exponent and the relative timing of the operations involved.
Surgical resection versus biopsy in the treatment of primary central nervous system lymphoma: a systematic review and meta-analysis
Authors: Chojak Rafał , Koźba-Gosztyła Marta , Polańska Karolina , Rojek Marta , Chojko Aleksandra , Bogacz Rafał , Skorupa Natalia , Więcław Jakub , Czapiga Bogdan 
Published: November 2022

Abstract
Purpose
Despite the improvement in treatment and prognosis of primary central nervous system lymphoma (PCNSL) over the last decades, the 5-year survival rate is approximately 30%; thus, new therapeutic approaches are needed to improve patient survival. The study’s aim was to evaluate the role of surgical resection of PCNSL.

Methods
Primary outcomes were the overall survival (OS) and progression-free survival (PFS) of patients with PCNSL who underwent surgical resection versus biopsy alone. The meta-analysis was conducted to calculate pooled hazard ratios (HRs) under a random-effects model for the time-to-event variables. The odds ratios (ORs) were calculated for binary, secondary outcome parameters.

Results
Seven studies (n = 1046) were included. We found that surgical resection was associated with significantly better OS (HR 0.63 [95% CI 0.51–0.77]) when compared with biopsy. PFS was also significantly improved (HR 0.64 [95% CI 0.49–0.85]) in patients who underwent resection compared with those who underwent biopsy. The heterogeneity for OS and PFS was low (I2 = 7% and 24%, respectively). We also found that patients who underwent biopsy more often had multiple (OR 0.38 [95% CI 0.19–0.79]) or deep-seated (OR 0.20 [95% CI 0.12–0.34]) lesions compared with those who underwent surgical resection. There were no significant differences in chemotherapy or radiotherapy use or the occurrence of postoperative complications between the two groups.

Conclusion
In selected patients, surgical resection of PCNSL is associated with significantly better overall survival and progression-free survival compared with biopsy alone.
Perceived negative consequences of dyslexia: the influence of person and environmental factors
Authors: Bazen Loes , de Bree Elise H. , van den Boer Madelon , de Jong Peter F. 
Published: November 2022

AbstractPerceived negative consequences of dyslexia entail the degree to which an individual perceives negative outcomes, such as low academic achievement or feelings of anxiety and depression, and attributes these experiences to the disorder. In the current study, we examined how perceived consequences of dyslexia are influenced by person and environmental factors. Perceived consequences were evaluated for the academic domain and the domain of mental health (depression, anxiety). Participants were 123 Dutch students with dyslexia. Cognitive person factors (literacy skills and verbal IQ), socio-emotional person factors (self-perceived literacy skills and coping ability), and environmental factors (literacy demands, support from the institution, reactions of teachers and peers) were included as predictors. Results indicated that perceived negative consequences were not related to cognitive person factors. In contrast, better self-perceived literacy skills were associated with less perceived negative consequences in all domains (academic, depression, anxiety) and coping contributed to depression consequences. With respect to environmental factors, negative reactions in the academic environment contributed to perceived negative consequences of depression and anxiety. As such, findings indicate that individuals with dyslexia perceive negative consequences in the academic, anxiety, and depression domains which cannot be fully accounted for by their objective reading and writing problems. These factors should feature more prominently in future studies on dyslexia and should be addressed in treatment of dyslexia as well.
Odor identification testing is inferior compared to neurocognitive testing in predicting conversion to Alzheimer's Disease
Authors: Pusswald G. , Ocak S. , Stögmann E. , Lehrner J. 
Published: November 2022

Abstract
Introduction
Alzheimer’s disease (AD) is a neurodegenerative disorder that affects memory, thinking, attention, and emotion or AD. Smelling problems are frequent symptoms of dementia. The aim of this study was to evaluate whether it is possible to predict if someone with anosmia or hyposmia has a higher risk of getting dementia or even AD.

Methods
This study was a retrospective longitudinal study, and the data used were part of a larger research project, the Vienna Conversion to Dementia Study. The 173 participants were divided into four groups based on cognitive features such as healthy control (HC), subjective cognitive decline (SCD), non-amnestic mild cognitive impairment (naMCI), and amnestic mild cognitive impairment (aMCI). Olfactory assessment and neurocognitive assessment were administered.

Results
We found that 30.5% of aMCI patients converted into AD after an average of about two years. The corresponding ROC analyses for olfactory testing showed that Sniffin’ Sticks revealed significant results regarding the conversion to AD, whereas the Assessment of Self-Reported Olfactory Functioning and olfaction-related quality of life (ASOF) inventory using the Subjective Olfactory Capability (SOC) subscale, the Smell-Related Problems (SRP) subscale, and the Olfaction-Related Quality of life (ORQ) did not. A logistic regression showed that among the olfactory test procedures, only the Sniffin’ Sticks enabled a relevant prognosis. Including neurocognitive measures in the model, only VSRT and the Trail Making Test-B. The other predictors did not contribute to the prediction of conversion to AD.

Conclusion
Unlike self-reporting of olfactory functioning, olfactory testing using standardized tests may have potential for predicting dementia, especially AD. However, olfactory tests have lower predictive power than neurocognitive tests such as verbal memory and divided attention tests.

Implications
Diagnostic tools for predicting dementia as accurately and early as possible are important. Olfactory assessment, compared to neurocognitive tests for verbal memory and divided attention, is inferior in predicting the prognosis of AD.
Nieuwe wegen naar arbocuratieve samenwerking: samen werken van praktijkondersteuners huis- en bedrijfsarts bij multiproblematiek
Authors: Vossen Emma , Schaafsma Frederieke G. , van der Gulden Joost W. J. , de Kock Cornelis A. , Schaap Rosanne , Anema Johannes R. , van Genabeek Joost A. G. M. 
Published: November 2022

SamenvattingAchtergrond:Momenteel is er nauwelijks sprake van arbocuratieve samenwerking tussen de eerstelijns- en bedrijfsgezondheidszorg. Waar eerdere initiatieven tot verbetering vooral gericht waren op de huis- en bedrijfsarts, onderzoeken we in deze bijdrage welke rol praktijkondersteuners van de huisarts (POH-ggz en POH-S) en van de bedrijfsarts (POB) voor zichzelf zien bij multiproblematiek. Tevens exploreren we welke belemmeringen er zijn voor arbocuratieve samenwerking door praktijkondersteuners bij multiproblematiek.Methode:We hebben drie focusgroepgesprekken uitgevoerd met zeven POH’s-ggz, elf POH’s‑S en acht POB’s – 26 praktijkondersteuners in totaal.Resultaten:De praktijkondersteuners in ons onderzoek komen tijdens hun werk in aanraking met multiproblematiek. POH’s en POB’s zien een rol voor zichzelf weggelegd bij het bespreken en het bieden van ondersteuning bij respectievelijk werk- en privégerelateerde klachten. Daarbij erkennen ze, waar nodig, het belang van arbocuratieve samenwerking om goede zorg te leveren. Op dit moment vindt er echter geen directe samenwerking plaats op het niveau van de praktijkondersteuner. Belemmeringen hiervoor blijken de formele regels rond taakdelegatie en rolopvatting van de POB, onbekendheid en vooroordelen bij vooral POH’s wat betreft de bedrijfsgezondheidszorg, en praktische barrières als de AVG-wetgeving en bereikbaarheid.Conclusie:POH’s en POB’s staan open voor arbocuratieve samenwerking, mits een oplossing gevonden wordt voor deze fundamentele en praktische belemmeringen.