bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Review on the Morphology, Nutritional Value, Traditional Uses, Phytochemistry, and Biological Activities of Pycnarrhena cauliflora and Its Synonyms
Authors: NOR AZMAN MUHAMMAD FARHAN SYAKIR , SALIM FATIMAH , NG SHEAN YEAW , ZAINURIN NUR AMIRAH ZAHIDAH , AZMI ADIEZ SAPURA , Suleiman Monica 
Published: November 2022

The leaves of Pycnarrhena cauliflora, locally known as ‘pokok Ajinomoto’, are consumed as flavour enhancers by East Malaysian communities. The plant also plays a significant role in traditional medicine especially to treat snake bites, eye irritation, headache, and seizures. An extensive literature search on the species revealed that besides taxonomic description as well as toxicity against HeLa human cervical and breast cancer cell lines, there is no report on the plant chemistry. The species has nevertheless been classified as the synonym of several other species which are Antitaxis cauliflora Miers., Pycnarrhena longifolia, (Decne. ex Miq.), Antitaxis longifolius (Decne. ex Miq.) Mier., and Gabila longifolia (Decne. ex Miq, B). Among these synonyms, the plant is closely related to the species P. longifolia. Thus, a complete review of P. cauliflora and its synonyms is important to revise and evaluate its potential for further studies and commercialisation. The information on the species was collected from scientific journals, books, and reports searched through available databases such as Google Scholar, PubMed, Directory of Open Access Journals, Science Direct, Bioline International and Reaxys. This review provides an insight on the morphology, nutritional value, traditional uses, phytochemistry, and biological activities of P. cauliflora and its synonyms published between 1981 to 2021 in scientific journals, books, and reports.
Pressurization System on Aircraft Boeing 737-300F, Aircraft MSN: 28567 During Flight Hanoi to Bangkok: Problem Solving and Root Cause Analysis
Authors: Simanjuntak Rexon Harris , Isdaryanto Isdaryanto 
Published: November 2022

The aircraft, with type Boeing 737-300 freighter, Serial Number: 28567, Flight number: TMG-918, with a flight route from Hanoi International (HAN) to Bangkok International airport (BKK) on the date of 12 April 2021, with a take-off time of 22.00 UTC and landing time 22.25 UTC, is operated by PT XYX Airlines on a non-schedule flight. After taking off from Hanoi to Bangkok (HAN – BKK), with an altitude of nearly 10.000 ft flight level, the aircraft got a problem with the pressurization system in auto mode (auto system), and the cabin altitude indicator was shown 10.000 ft; cabin rate altitude indication at 2000 – 300 ft/minute (in normal rate 200 – 300 ft/minute). The pilot was unable to control the pressurization system in the auto system; in this condition, the Pilot followed the procedure for selecting the pressurization system from auto mode to manual mode, and after that, the cabin altitude indicator suddenly was shown fluctuated or became unstable. After the pressurization system operates in Manual mode and all conditions control by the flight crew, the captain makes the decision to aircraft return to base (RTB) flight back to Hanoi International airport (HAN) and land safely at 22.25 UTC. The author wants to analyse the root cause of the pressurization system problem by using several methods, as per 5 whys, fishbone diagrams, and fault tree analysis. Then the author compares the root cause results of each method to take corrective and preventive actions to solve the problems. All these cases were in Reference to SDR Report Control Number: 012/SDR-YGH/TMG/IV/2021. The report submitted by PT.Tri M.G. Intra Asia Airlines on 12 April 2021 and the Flight Log Manual (FML) Page No: 03232, dated 12 April 2021. The purpose of this research is to identify the problem and determine the root cause of the problem's inability to control the pressurization system and make a plan for corrective action and preventive action; then, the team will implement the corrective action and preventive action. Quality Division will check and monitor the effectiveness of corrective and preventive action in three months. After that, the team & Quality Division create the procedure and policy for the company standard. The author and team used the methodology 5 why (cause and effect diagram), Fishbone diagram (Ishikawa Diagram), and Fault Tree Analysis (FTA), and from each method will be found the root cause analysis. Then the author combines all root cause analyses to identify the problem and create problem-solving. The main result of this research was that the company was able to determine and implement the corrective action and preventive action for the uncontrollable problem pressurization system. The problem of the pressurization system that cannot be controlled causes the aircraft to return to base or cancel a flight to be expected not to happen again in all aircraft operated by PT. XYX company in the future.
Ekstrak Daun Sirih untuk Keputihan pada Wanita Usia Subur di SMA Negeri 1 Wanayasa Purwakarta
Authors: Yulianti Lia , Rahayu Sri , Wahidin Wahidin , Raidanti Dina 
Published: November 2022

Keputihan merupakan masalah kesehatan yang sering ditemukan pada wanita. Penyebab keputihan adalah masalah kebersihan pada area organ intim seperti kurang bersih pada saat membersihkan organ intim setelah buang air kecil dan buang air besar. Daun sirih mengandung minyak atsiri, chavikol yang menyebabkan daun sirih memiliki bau yang khas dan memilik khasiat untuk membunuh bakteri yang merupakan tanaman obat tradisional yang telah diketahui efektif dalam mengobati berbagai jenis penyakit secara tradisional. Penelitian ini bertujuan untuk mengetahui manfaat ekstra daun sirih hijau untuk keputihan pada wanita usia subur di SMA Negeri 1 Wanayasa Purwakarta. penelitian ini adalah rancangan Pra Eksperimen (Pre-Experiment Design) dengan menggunakan desain penelitian Case Control, jumlah sampel yang di ambil yaitu sebanyak 30 orang responden dengan menggunakan teknik purposive sampling. Hasil penelitian ini menunjukkan rata-rata keputihan yang dialami sebelum diberikan perlakuan adalah 60.00. Setelah dilakukan perlakuan meminum daun sirih dua kali sehari selama jangka waktu seminggu rata-rata keputihan yang dialami adalah 86.7. p-value 0,041. Kesimpulan penelitian ini yaitu ada efek meminum air rebusan daun sirih terhadap keputihan pada siswi SMA 1Wanayasa Purwakarta. Dapat disarankan kepada wanita usia subur perlu memanfaatkan ekstra daun siri untuk mengurangi masalah keputihan salah satu caranya dengan meminum air rebusan daun sirih.
Biomechanical effects of individualized artificial titanium alloy lamina implantation after laminectomy: A finite element analysis
Authors: Gong Xuqiang , Zhang Aobo , Han Qing , Wang Yang , Liu Yang , Jiao Jianhang , Yue Jing , Chen Hao , Luo Wangwang , Wang Jincheng , Wu Minfei 
Published: November 2022

Background and objectives: Laminectomy is a common surgical procedure in spine surgery. However, disruption of the posterior ligamentous complex of the spine may lead to a range of postoperative complications. Artificial lamina as a kind of bionic implant can well restore the posterior spinal structure. In this study, an individualized artificial titanium alloy lamina was designed to reconstruct the posterior spinal structure after laminectomy and explored its biomechanical effects, which could provide a theoretical basis for the clinical application of the artificial lamina.Methods: Three finite element models were constructed, namely the nonlinear and non-homogeneous intact model of the whole lumbar spine, the lumbar decompression alone surgical model, and the artificial lamina implantation surgical model. The range of motion, intradiscal pressure, and annulus fibrosus peak stress were compared between the three models at the surgical and adjacent segments. The stresses of the artificial lamina and fixation screws were also analyzed for the four movement states.Results: Compared with the intact model, the lumbar decompression alone surgical model showed an increase in range of motion, intradiscal pressure, and annulus fibrosus peak stresses at the surgical segment and adjacent segments under all conditions. The artificial lamina implantation surgical model showed an increase in these measurements only in flexion, increasing by 7.5%–22.5%, 7.6%–17.9%, and 6.4%–19.3%, respectively, over the intact model, while there was little difference under other conditions. The peak stresses in both the screw and the artificial lamina were highest in axial rotation, i. e. 46.53 MPa and 53.84 MPa, respectively. Screw stresses were concentrated on the connection between the screw and the artificial lamina, and artificial lamina stresses were concentrated on the spinous root, around the screw hole, and the contact with the vertebral body.Conclusion: An individualized artificial titanium alloy lamina can effectively reduce the range of motion, intradiscal pressure, and annulus fibrosus stress at the surgical segment and adjacent segments. The application of artificial lamina could better preserve the biomechanical properties of the intact lumbar spine and reduce the risk of adjacent segmental disease.
Two genetic variants in NEXN and ABCC6 genes found in a patient with right coronary artery to right ventricle fistula combined with giant coronary aneurysm and patent ductus arteriosus
Authors: Peng Yongxuan , Ye Jiajun , Xu Yuejuan , Huang Jihong , Wu Yurong , Liu Wei , Bai Kai , Chen Sun , Lu Yanan 
Published: November 2022

ObjectiveCoronary artery fistula, defined as communication between a coronary artery and a great vessel or a cardiac chamber, is a relatively rare anomaly with an estimated incidence of 0.002% in the general population. It could be combined with a giant coronary artery aneurysm, with an incidence of 5.9% of the total incidence rate of CAF in the general population. The pathogenesis of these two combined anomalies is not clear, and we aimed to detect whether genetic abnormalities underlie the pathogenesis of these rarely combined anomalies.Materials and methodsA 6-year-old patient with a diagnosis of the right coronary artery to right ventricle fistula combined with a giant right coronary artery aneurysm and patent ductus arteriosus underwent a surgical repair at our center. The diagnosis was confirmed by echocardiography, CT, and surgery. DNA was extracted from the peripheral venous blood samples of the patient and his mother after informed consent was obtained. Hematoxylin and Eosin (HE) and Alizarin red staining were performed on the excised coronary artery aneurysm. Exome sequencing and in silico analyses were performed to detect detrimental genetic variants.ResultsNo obvious abnormalities were found in the excised coronary artery aneurysm. A heterozygous truncated variant (NM_144573: c.G298T; p.G100X) in the NEXN gene and a missense variant (NM_001171: c.G1312A; p.V438M) in the ABCC6 gene were carried by the patient but not by his mother.ConclusionThe NEXN-truncated variant, NEXN-G100X, is associated with the development of coronary arteries and congenital coronary artery anomalies.
Identification and verification of immune-related biomarkers and immune infiltration in diabetic heart failure
Authors: Zhong Zuoquan , Zhang Hanlin , Xu Ting , Hao Jinjin , Chen Xing , Sun Shimin , Yang Jinjin , Sun Jing , Lin Hui , Guo Hangyuan 
Published: November 2022

PurposeDiabetic heart failure (DHF) or cardiomyopathy is a common complication of diabetes; however, the underlying mechanism is not clear. In the present study, the authors searched for differentially expressed genes associated with DHF and the molecular types of immune cells based on bioinformatics.MethodsThe RNA expression dataset of DHF was obtained from the NCBI Gene Expression Omnibus (GEO) database. After preprocessing the data, the differentially expressed genes (DEGs) between the DHF group and the non-diabetic heart failure (NHF) group were screened and intersected with immune-related genes (IRGs) in the ImmPort database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed using the DAVID tool. The ssGSEA algorithm was used to evaluate immune infiltration of the heart tissue in each group. In addition, the protein-protein interaction (PPI) network and miRNA-mRNA network were constructed using the STRING online website and Cytoscape program. Finally, validation analysis was performed using animal models.ResultsEight immune-related core genes were identified. GO and KEGG showed that core genes were mainly enriched in angiogenesis and cytokine-cytokine receptor interaction. Immune infiltration results showed that activated dendritic cells, central memory CD4 T cells, central memory CD8 T cells, myeloid-derived suppressor cells (MDSCs), neutrophils, and regulatory T cells may be involved in DHF. Neutrophils may play a key role in the pathogenesis of HF in diabetes.ConclusionImmune-related core genes and immune infiltrating cells provide a new perspective on the pathogenesis of DHF.
Prognostic value of 18F-FDG PET/CT in patients with advanced or metastatic non-small-cell lung cancer treated with immune checkpoint inhibitors: A systematic review and meta-analysis
Authors: Ling Tao , Zhang Lianghui , Peng Rui , Yue Chao , Huang Lingli 
Published: November 2022

PurposeThis study aimed to investigate the value of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) in predicting early immunotherapy response of immune checkpoint inhibitors (ICIs) in patients with advanced or metastatic non-small-cell lung cancer (NSCLC).MethodsA comprehensive search of PubMed, Web of science, Embase and the Cochrane library was performed to examine the prognostic value of 18F-FDG PET/CT in predicting early immunotherapy response of ICIs in patients with NSCLC. The main outcomes for evaluation were overall survival (OS) and progression-free survival (PFS). Detailed data from each study were extracted and analyzed using STATA 14.0 software.Results13 eligible articles were included in this systematic review. Compared to baseline 18F-FDG PET/CT imaging, the pooled hazard ratios (HR) of maximum and mean standardized uptake values SUVmax, SUVmean, MTV and TLG for OS were 0.88 (95% CI: 0.69-1.12), 0.79 (95% CI: 0.50-1.27), 2.10 (95% CI: 1.57-2.82) and 1.58 (95% CI: 1.03-2.44), respectively. The pooled HR of SUVmax, SUVmean, MTV and TLG for PFS were 1.06 (95% CI: 0.68–1.65), 0.66 (95% CI: 0.48-0.90), 1.50 (95% CI: 1.26-1.79), 1.27 (95% CI: 0.92-1.77), respectively. Subgroup analysis showed that high MTV group had shorter OS than low MTV group in both first line group (HR: 1.97, 95% CI: 1.39-2.79) and undefined line group (HR: 2.11, 95% CI: 1.61-2.77). High MTV group also showed a shorter PFS in first line group (HR: 1.85, 95% CI: 1.28-2.68), and low TLG group had a longer OS in undefined group (HR: 1.37, 95% CI: 1.00-1.86). No significant differences were in other subgroup analysis.ConclusionBaseline MTV and TLG may have predictive value and should be prospectively studied in clinical trials. Baseline SUVmax and SUVmean may not be appropriate prognostic markers in advanced or metastatic NSCLC patients treated with ICIs.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=323906, identifier CRD42022323906.
Proteomic analysis of urinary extracellular vesicles of kidney transplant recipients with BKV viruria and viremia: A pilot study
Authors: Bruschi Maurizio , Granata Simona , Candiano Giovanni , Petretto Andrea , Bartolucci Martina , Ghiggeri Gian Marco , Stallone Giovanni , Zaza Gianluigi 
Published: November 2022

IntroductionTo better define the biological machinery associated with BK virus (BKV) infection, in kidney transplantation, we performed a proteomics analysis of urinary extracellular vesicles (EVs).MethodsTwenty-nine adult kidney transplant recipients (KTRs) with normal allograft function affected by BKV infection (15 with only viremia, 14 with viruria and viremia) and 15 controls (CTR, KTRs without BKV infection) were enrolled and randomly divided in a training cohort (12 BKV and 6 CTR) used for the mass spectrometry analysis of the EVs (microvesicles and exosomes) protein content and a testing cohort (17 BKV and 9 CTR) used for the biological validation of the proteomic results by ELISA. Bioinformatics and functional analysis revealed that several biological processes were enriched in BKV (including immunity, complement activation, renal fibrosis) and were able to discriminate BKV vs. CTR. Kinase was the only gene ontology annotation term including proteins less abundant in BKV (with SLK being the most significantly down-regulated protein). Non-linear support vector machine (SVM) learning and partial least squares discriminant analysis (PLS-DA) identified 36 proteins (including DNASE2, F12, AGT, CTSH, C4A, C7, FABP4, and BPNT1) able to discriminate the two study groups. The proteomic profile of KTRs with BKV viruria alone vs. viremia and viruria was quite similar. Enzyme-linked immunosorbent assay (ELISA) for SLK, BPNT1 and DNASE2, performed on testing cohort, validated proteomics results.DiscussionsOur pilot study demonstrated, for the first time, that BKV infection, also in the viruric state, can have a negative impact on the allograft and it suggested that, whether possible, an early preventive therapeutic strategy should be undertaken also in KTRs with viruria only. Our results, then, revealed new mechanistic insights into BKV infection and they selected potential biomarkers that should be tested in future studies with larger patients’ cohorts.
Risk factors of lymph node metastasis or lymphovascular invasion for superficial esophageal squamous cell carcinoma: A practical and effective predictive nomogram based on a cancer hospital data
Authors: Tao Yali , Chen Shengsen , Yu Jiangping , Shen Qiwen , Ruan Rongwei , Wang Shi 
Published: November 2022

BackgroundLymphovascular invasion (LVI) is mostly used as a preoperative predictor to establish lymph node metastasis (LNM) prediction models for superficial esophageal squamous cell carcinoma (SESCC). However, LVI still needs to be confirmed by postoperative pathology. In this study, we combined LNM and LVI as a unified outcome and named it LNM/LVI, and aimed to develop an LNM/LVI prediction model in SESCC using preoperative factors.MethodsA total of 512 patients who underwent radical resection of SESCC were retrospectively collected. Logistic regression and least absolute shrinkage and selection operator (LASSO) regression were adopted to identify the predictive factors of LNM/LVI. Integrated discrimination improvement (IDI) and net reclassification improvement (NRI) were calculated to select the potential predictive factors from the results of LASSO and logistic regression. A nomogram for predicting LNM/LVI was established by incorporating these factors. The efficacy, accuracy, and clinical utility of the nomogram were, respectively, assessed with the area under the curve (AUC), calibration curve, and decision curve analysis (DCA). Finally, the random forest (RF) algorithm was used to further evaluate the impact of these factors included in the nomogram on LNM/LVI.ResultsTumor size, tumor location, tumor invasion depth, tumor differentiation, and macroscopic type were confirmed as independent risk factors for LNM/LVI according to the results of logistic regression, LASSO regression, IDI, and NRI analyses. A nomogram including these five variables showed a good performance in LNM/LVI prediction (AUC = 0.776). The calibration curve revealed that the predictive results of this nomogram were nearly consistent with actual observations. Significant clinical utility of our nomogram was demonstrated by DCA. The RF model with the same five variables also had similar predictive efficacy with the nomogram (AUC = 0.775).ConclusionThe nomogram was adopted as a final tool for predicting LNM/LVI because its risk score system made it more user-friendly and clinically useful than the random forest model, which can help clinicians make optimal treatment decisions for patients with SESCC.
Multiple sexually transmitted co-infections are associated with adverse reproductive outcomes in asymptomatic adolescent pregnant women; A Prospective cohort study
Authors: Wasnik Kirti , Mittal Pratima , Ghope Priti , Sonkar Subash C. , Arora Geetika , Saluja Daman 
Published: November 2022

BackgroundA prospective cohort was conducted to assess the prevalence of seven RTIs/STIs in adolescent asymptomatic pregnant women to find a significant correlation between infection and pregnancy.MethodsThe study was restricted to 18–19 years of asymptomatic adolescent pregnant women attending Ante-Natal Care and the health status of the pregnant women were followed up to parturition. The health status of the infant was followed till 6 months post-delivery. The prevalence of the concerning pathogens and the significance of their association with adverse outcomes of pregnancy were determined.ResultsAmong 279 subjects, the most significant co-infections were observed for M. hominis with U. parvum (9.31%; p-value–0.0071/OR−2.6421) and U. urealyticum (7.88%; p-value–0.0119/OR−2.6455). Statistically significant associations were found between C. trachomatis [(p-value-0.0439); OR−2.9902] and M. genitalium [(p-value−0.0284); OR−3.442] with PTB, N. gonorrhoeae with LBW &lt;2.5 kg [(p-value−0.0052);OR−4.9017], U. urealyticum with VLBW &lt;2 kg [(p-value-0.0262);OR−3.0207], M. genitalium [(p-value-0.0184); OR−11.7976] and T. vaginalis with PROM [(p-value 0.0063); OR−19.4275] while M. genitalium [(p-value 0.0190); OR–12.9230] and U. urealyticum [(p-value 0.0063); OR-14.5149] with PPROM with 95% CI respectively.ConclusionsAsymptomatic adolescents are at high risk of adverse pregnancy outcomes if infected with the concerned pathogens.
The clinical implications of circulating microRNAs as potential biomarkers in screening oral squamous cell carcinoma
Authors: Gao Huan , Shen Yi , Feng Zhengyang , Cai Yuxing , Yang Jianxin , Zhu Yaqun , Peng Qiliang 
Published: November 2022

BackgroundRecent studies have highlighted the biomarker role of circulating miRNAs in oral squamous cell carcinoma (OSCC), indicating their potential application as early diagnostic markers for OSCC. However, the diagnostic results have proven inconclusive. This study was conducted to evaluate the diagnostic value of circulating miRNAs for OSCC diagnosis.MethodsEligible published studies were identified by a literature search carried out in several databases by using combinations of keywords associated with OSCC, circulating miRNAs, and diagnosis. The bivariate meta-analysis model was adopted to summarize the pooled parameters. Afterwards, we thoroughly explored the sources of heterogeneity after evaluating the risk of bias.ResultsA total of 60 studies focusing on 41 circulating miRNAs were included. The pooled sensitivity, specificity, and AUC were 0.75 (95%CI: 0.69-0.80), 0.76 (0.70-0.81), 0.82 (0.79-0.85), respectively. Subgroup analyses showed that miRNA combinations were more accurate than single miRNAs. Additionally, plasma may be a better matrix for miRNAs assays in OSCC diagnosis as the plasma-based miRNA assay had a higher level of diagnostic accuracy than serum-based miRNA assay. Subgroup analyses also suggested that using circulating miRNAs for OSCC diagnosis is more effective in Caucasians than in Asian ethnic groups. Finally, circulating miRNA assays based on large sample sizes have superior diagnostic accuracy than small sample sizes.ConclusionCirculating miRNAs might be applied as effective surrogate biomarkers for early diagnosis of OSCC. Nevertheless, future larger-scale prospective studies should be performed to enhance the diagnostic efficiency and investigate the miRNA combinations with more pronounced accuracy.