bluuebunny/crossref_metadata_2025_split huggingface dataset:
Task prioritization modulates low frequency EEG dynamics reflecting proactive cognitive control
Authors: Liegel Nathalie , Schneider Daniel , Wascher Edmund , Arnau Stefan 
Published: May 2022

AbstractMost neuroscientific studies investigating mental effort apply unspecific effort allocation paradigms. In contrast, the present EEG study targets specific effort allocation during task prioritization.Twenty-eight participants performed a cued number classification task during the retention interval of a working memory task including retrospective cues. One of two possible number classifications was done per trial. Each trial started with a cue indicating which of the two tasks would be more important in the upcoming trial. Subjects were told to engage in both tasks, but to concentrate on the important one. Feedback given at the end of each trial was calculated based on task performance, with scores obtained from the relevant task being tripled.Participants performed significantly better in either task when it was important compared to when not.Task prioritization modulates theta, alpha and beta oscillations, predominantly during task preparation. Multivariate pattern analysis revealed that the exact type of the two possible number classifications was decodable, however, decoding accuracy did not depend on task importance. Hemispheric alpha power asymmetries indicating attentional orienting between working memory representations also did not depend on task importance. The findings suggest that task prioritization primarily affects proactive cognitive control on a superordinate level.
Heading direction tracks internally directed selective attention in visual working memory
Authors: Thom Jude L. , Nobre Anna C. , van Ede Freek , Draschkow Dejan 
Published: May 2022

AbstractWe shift our gaze even when we orient attention internally to visual representations in working memory. Here, we show the bodily orienting response associated with internal selective attention is widespread as it also includes the head. In three virtual reality (VR) experiments, participants remembered two visual items. After a working memory delay, a central colour cue indicated which item needed to be reproduced from memory. After the cue, head movements became biased in the direction of the memorised location of the cued memory item – despite there being no items to orient towards in the external environment. The heading-direction bias had a distinct temporal profile from the gaze bias. Our findings reveal that directing attention within the spatial layout of visual working memory bears a strong relation to the overt head orienting response we engage when directing attention to sensory information in the external environment. The heading-direction bias further demonstrates common neural circuitry is engaged during external and internal orienting of attention.
Elemental content of a host-parasite relationship in the threespine stickleback
Authors: Braat Megan , Grunberg Rita L. , Bolnick Daniel I. 
Published: May 2022

AbstractParasite infections are ubiquitous and their effects on hosts may play a role in ecosystem processes. Ecological stoichiometry provides a framework to study linkages between consumers and ecosystem process, but the stoichiometric traits of host-parasite associations are rarely quantified. Specifically, whether parasites’ elemental ratios closely resemble those of their host or if infection is related to host stoichiometry remains less known. To answer such questions, we measured the elemental content (%C, %N, and %P) and ratios (C:N, C:P, and N:P) of parasitized and unparasitized Gasterosteus aculeatus (three-spined stickleback) and their cestode parasite, Schistocephalus solidus. Host and parasite elemental content were distinct from each other, and parasites were generally higher in %C and lower in %N and %P. Parasite infections were related to some elemental ratios, specifically C:N, with more intense parasite infections corresponding to hosts with lower C:N ratio. Parasite stoichiometry was independent of their host and there was no relationship between host and parasite stoichiometry. Instead, parasite body mass and parasite density were important drivers of parasite stoichiometry where larger parasites had lower %C, %N, and %P,. Overall, these potential effects of parasite infections on host stoichiometry along with parasites’ distinct elemental compositions suggest parasites may further contribute to how hosts store and cycle nutrients.
A potent and selective inhibitor for the modulation of MAGL activity in the neurovasculature
Authors: Kemble Alicia , Hornsperger Benoit , Ruf Iris , Richter Hans , Benz Jörg , Kuhn Bernd , Heer Dominik , Wittwer Matthias , Engelhardt Britta , Grether Uwe , Collin Ludovic 
Published: May 2022

ABSTRACTChronic inflammation and blood–brain barrier dysfunction are key pathological hallmarks of neurological disorders such as multiple sclerosis, Alzheimer’s disease and Parkinson’s disease. Major drivers of these pathologies include pro-inflammatory stimuli such as prostaglandins, which are produced in the central nervous system by the oxidation of arachidonic acid in a reaction catalyzed by the cyclooxygenases COX1 and COX2. Monoacylglycerol lipase hydrolyzes the endocannabinoid signaling lipid 2-arachidonyl glycerol, enhancing local pools of arachidonic acid in the brain and leading to cyclooxygenase-mediated prostaglandin production and neuroinflammation. Monoacylglycerol lipase inhibitors were recently shown to act as effective anti-inflammatory modulators, increasing 2-arachidonyl glycerol levels while reducing levels of arachidonic acid and prostaglandins, including PGE2 and PGD2. In this study, we characterized a novel, highly selective, potent and reversible monoacylglycerol lipase inhibitor (MAGLi 432) in a mouse model of lipopolysaccharide-induced blood–brain barrier permeability and in both human and mouse cells of the neurovascular unit: brain microvascular endothelial cells, pericytes and astrocytes. We confirmed the expression of monoacylglycerol lipase in specific neurovascular unit cells in vitro, with pericytes showing the highest expression level and activity. However, MAGLi 432 did not ameliorate lipopolysaccharide-induced blood–brain barrier permeability in vivo or reduce the production of pro-inflammatory cytokines in the brain. Our data confirm monoacylglycerol lipase expression in mouse and human cells of the neurovascular unit and provide the basis for further cell-specific analysis of MAGLi 432 in the context of blood–brain barrier dysfunction caused by inflammatory insults.
The effect of dietary chitin on Atlantic salmon (Salmo salar) chitinase activity, gene expression, and microbial composition
Authors: Holen Matilde Mengkrog , Sandve Simen Rød , Harvey Thomas Nelson , Jin Yang , Angell Inga Leena , Rudi Knut , Kent Matthew Peter 
Published: May 2022

ABSTRACTBackgroundChitin is a common component in the natural diet of many fish, and a range of chitinases with the potential to down chitin have been identified. Yet whether chitin is metabolized in fish is still unclear. Here we used a combination of chitinase activity assay, transcriptomics, and 16S rRNA bacterial analysis to assess the effect of chitin supplementation on Atlantic salmon gene expression and microbial community.ResultsAtlantic salmon express multiple genes associated with chitin metabolism, and we show that the expression and activity of Atlantic salmon chitinases are not affected by the addition of dietary chitin. We do, however, demonstrate an association between gut microbial composition, chitinase activity in the gut, and host chitinase expression.ConclusionThe findings presented here support the idea that chitin metabolism genes are linked to the maintenance of a chitin-based barrier in the teleost gut. These results contribute to a greater understanding of chitin metabolism in fish.
Early patterning followed by tissue growth establishes proximo-distal identity in Drosophila Malpighian tubules
Authors: Beaven Robin , Denholm Barry 
Published: May 2022

AbstractSpecification and elaboration of proximo-distal (P-D) axes for structures or tissues within a body occurs secondarily from that of the main axes of the body. Our understanding of the mechanism(s) that pattern P-D axes is limited to a few examples such as vertebrate and invertebrate limbs. Drosophila Malpighian/renal tubules (MipTs) are simple epithelial tubules, with a defined P-D axis. How this axis is patterned is not known, and provides an ideal context to understand patterning mechanisms of a secondary axis. Furthermore, epithelial tubules are widespread, and their patterning is not well understood. Here, we describe the mechanism that establishes distal tubule and show this is a radically different mechanism to that patterning the proximal MpT. The distal domain is patterned in two steps: distal identity is specified in a small group of cells at the earliest stage of MpT development through Wingless/Wnt signalling. Subsequently, this population is expanded by proliferation to generate the distal MpT domain. This mechanism enables distal identity to be established in the tubule in a domain of cells much wider than the effective range of Wingless.Summary statementHow does proximo-distal patterning occur in the epithelial Malpighian/renal tubules? Cells are patterned early by a mechanism involving Wingless/Wnt, and expand by cell proliferation to generate a distal domain.
Developmental Morphogens Direct Human Induced Pluripotent Stem Cells Towards an Annulus Fibrosus-Like Cell Phenotype
Authors: Peredo Ana P. , Tsinman Tonia K. , Bonnevie Edward D. , Jiang Xi , Smith Harvey E. , Gullbrand Sarah E. , Dyment Nathaniel A. , Mauck Robert L. 
Published: May 2022

AbstractTherapeutic interventions for intervertebral disc herniation remain scarce due to the inability of endogenous annulus fibrosus (AF) cells to respond to injury and drive tissue regeneration. Unlike other orthopaedic tissues, such as cartilage, delivery of exogenous cells to the site of annular injury remains underdeveloped, largely due to a lack of an ideal cell source and the invasive nature of cell isolation. Human induced pluripotent stem cells (iPSCs) can be differentiated to specific cell fates using biochemical factors and are, therefore, an invaluable tool for cell therapy approaches. While differentiation protocols have been developed for cartilage and fibrous connective tissues (e.g., tendon), the signals that regulate the induction and differentiation of human iPSCs towards the annulus fibrosus fate remain unknown. Here, we screened a number of candidate factors (and their combinations) and assessed the transcriptomic signatures of key signaling factors involved in embryonic AF development and differentiated function. The transcriptional signatures of treated cells were compared to those of mature human AF cells, and conditions that promoted expression of annulus fibrosus extracellular matrix genes and key transcription factors involved in embryonic AF development were identified. These findings represent an initial approach to guide human induced pluripotent stem cells towards an annulus fibrosus-like fate for cellular delivery strategies.
Mechanism of the cadherin-catenin F-actin catch bond interaction
Authors: Wang Amy , Dunn Alexander R. , Weis William I. 
Published: May 2022

AbstractMechanotransduction at cell-cell adhesions is crucial for the structural integrity, organization, and morphogenesis of epithelia. At cell-cell junctions, ternary E-cadherin/β-catenin/αE-catenin complexes sense and transmit mechanical load by binding to F-actin. The interaction with F-actin, described as a two-state catch bond, is weak in solution but is strengthened by applied force due to force-dependent transitions between weak and strong actin-binding states. Here, we provide direct evidence from optical trapping experiments that the catch bond property principally resides in the αE-catenin actin-binding domain (ABD). Consistent with our previously proposed model, deletion of the first helix of the five-helix ABD bundle enables stable interactions with F-actin under minimal load that are well-described by a single-state slip bond, even when αE-catenin is complexed with β-catenin and E-cadherin. Our data argue for a conserved catch bond mechanism for adhesion proteins with structurally similar ABDs. We also demonstrate that a stably bound ABD strengthens load-dependent binding interactions between a neighboring complex and F-actin, but the presence of the other αE-catenin domains weakens this effect. These results provide mechanistic insight to the cooperative binding of the cadherin-catenin complex to F-actin, which regulate dynamic cytoskeletal linkages in epithelial tissues.
Expansion of information in scientific research papers
Authors: Abdullaeva Malika , Bromfield John J. , Sheldon I. Martin 
Published: May 2022

AbstractPresenting information in papers allows readers to see the evidence for the research claims. The amount of information presented to readers is increasing in high impact factor scientific journals. The aim of the present study was to determine whether there was a similar expansion in the amount of information presented to readers in subject-specific journals. We examined 878 research papers that were published in the journals Biology of Reproduction and Reproduction during the first six months of 1989, 1999, 2009, and 2019. Although there were few differences between the journals, we found that between 1989 and 2019 the number of figures increased 1.5-fold, the number of figure panels increased 3.6-fold, and the number of display items increased 5.6-fold. Amongst the display items, the number of images per paper increased 10-fold, and the number of graphs per paper increased 3.7-fold. The median paper in 1989 was 8 pages long, contained 6 tables and/or figures, with 1 image and 4 graphs. In 2019 the median paper was 12 pages long, contained 7 tables and/or figures, with 13 images and 15 graphs. This expansion of information in subject-specific journals implies that authors, reviewers, and editors need to help readers digest complex biological messages without causing information overload.Lay summaryWe are living in an age of science and information. The amount of information presented in research papers has increased over time in the top science journals. Our research examined whether there has been a similar expansion in information in two influential subject-specific journals. We counted how much information was presented in 878 research papers across a 30-year period in the journals Biology of Reproduction and Reproduction. There were few differences between the two journals. But there was a striking increase in the information presented to readers in 2019 compared with 1989. The typical paper in 1989 was 8 pages long and contained 1 picture and 4 graphs. In 2019 the typical paper was 12 pages long and contained 13 pictures and 15 graphs. This expansion of information means that subject-specific journals must balance the presentation of complex biological messages with the risk of causing information overload.
A curated data resource of 214K metagenomes for characterization of the global resistome
Authors: Martiny Hannah-Marie , Munk Patrick , Brinch Christian , Aarestrup Frank M. , Petersen Thomas N. 
Published: May 2022

AbstractThe growing threat of antimicrobial resistance (AMR) calls for new epidemiological surveillance methods, as well as a deeper understanding of how antimicrobial resistance genes (ARGs) have transmitted around the world. The large pool of sequencing data available in public repositories provides an excellent resource for monitoring the temporal and spatial dissemination of AMR in different ecological settings. However, only a limited number of research groups globally have the computational resources allowing analyses of such data. We retrieved 442 Tbp of sequencing reads from 214,095 metagenomic samples from the European Nucleotide Archive (ENA) and aligned them using a uniform approach against ARGs and 16S/18S rRNA genes. Here, we present the results of this extensive computational analysis and share the counts of reads aligned. Over 6.76 · 108read fragments were assigned to ARGs and 3.21 · 109to rRNA genes, where we observed distinct differences in both the abundance of ARGs and the link between microbiome and resistome compositions across various sampling types. This collection is another step towards establishing a global surveillance of AMR and can serve as a resource for further research into the environmental spread and dynamic changes of ARGs.
Protrusion growth driven by myosin-generated force
Authors: Fitz Gillian N. , Weck Meredith L. , Bodnya Caroline , Perkins Olivia L. , Tyska Matthew J. 
Published: May 2022

SUMMARYActin-based protrusions are found on the surface of all eukaryotic cells, where they support diverse biological activities essential for life. Models of protrusion growth hypothesize that actin filament assembly provides the mechanical force for bending the plasma membrane outward. However, membrane-associated myosin motors are also abundant in protrusions, though their potential for contributing growth-promoting force remains unexplored. Using a novel inducible system that docks myosin motor domains to membrane binding modules with temporal control, we found that the application of myosin-generated force to the plasma membrane is sufficient for driving robust elongation of protrusions. Protrusion growth scaled with motor accumulation, required active, barbed end-directed force, and was independent of cargo delivery or the recruitment of canonical barbed end elongation factors. Application of growth-promoting force was also supported by structurally distinct myosin motor domains and membrane binding modules. We conclude that myosin-generated force can drive protrusion growth and this mechanism is likely active in diverse biological contexts.
The structure and molecular dynamics of prolyl oligopeptidase from Microbulbifer arenaceous provide insights into catalytic and regulatory mechanisms
Authors: Huang Ping , Lv Ang , Yan Qiaojuan , Jiang Zhengqiang , Yang Shaoqing 
Published: May 2022

Prolyl oligopeptidases (POPs) are atypical serine proteases that are unique in their involvement in the maturation and degradation of prolyl-containing peptide hormones and neuropeptides. They are potential pharmaceutical targets for the treatment of several neurodegenerative disorders, such as Alzheimer's disease. In this study, the catalytic and substrate-regulatory mechanisms of a novel bacterial POP from Microbulbifer arenaceous (MaPOP) were investigated. The crystal structure revealed that the catalytic triad of MaPOP was covered by the central tunnel of an unusual β-propeller domain. The tunnel not only provided the sole access to the active site for oligopeptides, but also protected large structured peptides or proteins from accidental proteolysis. The enzyme was able to cleave angiotensin I specifically at the carboxyl side of the internal proline residue, but could not hydrolyze long-chain bovine insulin B in vitro. Like the ligand-free structure, MaPOP bound to the transition-state analog inhibitor ZPR was also in a closed state, which was not modulated by the common `latching loop' found in other POPs. The substrate-assisted catalytic mechanism of MaPOP reported here may represent a common mechanism for all POPs. These results may facilitate a better understanding of the catalytic behavior of POPs under physiological conditions.
Realizing the promise of big data: how Taiwan can help the world reduce medical errors and advance precision medicine
Authors: Wang Kevin , Muennig Peter Alexander 
Published: May 2022

PurposeThe study explores how Taiwan’s electronic health data systems can be used to build algorithms that reduce or eliminate medical errors and to advance precision medicine.Design/methodology/approachThis study is a narrative review of the literature.FindingsThe body of medical knowledge has grown far too large for human clinicians to parse. In theory, electronic health records could augment clinical decision-making with electronic clinical decision support systems (CDSSs). However, computer scientists and clinicians have made remarkably little progress in building CDSSs, because health data tend to be siloed across many different systems that are not interoperable and cannot be linked using common identifiers. As a result, medicine in the USA is often practiced inconsistently with poor adherence to the best preventive and clinical practices. Poor information technology infrastructure contributes to medical errors and waste, resulting in suboptimal care and tens of thousands of premature deaths every year. Taiwan’s national health system, in contrast, is underpinned by a coordinated system of electronic data systems but remains underutilized. In this paper, the authors present a theoretical path toward developing artificial intelligence (AI)-driven CDSS systems using Taiwan’s National Health Insurance Research Database. Such a system could in theory not only optimize care and prevent clinical errors but also empower patients to track their progress in achieving their personal health goals.Originality/valueWhile research teams have previously built AI systems with limited applications, this study provides a framework for building global AI-based CDSS systems using one of the world’s few unified electronic health data systems.