bluuebunny/crossref_metadata_2025_split huggingface dataset:
Localized expression and role of full-length growth differentiation factor 15 in non-small cell lung cancer
Authors: Morita-Tanaka Satomi , Miyagawa-Hayashino Aya , Yamada Tadaaki , Matsui Yohei , Morimoto Kenji , Hiranuma Osamu , Masuzawa Naoko , Yoshimura Akihiro , Iwasaku Masahiro , Tokuda Shinsaku , Kaneko Yoshiko , Kim Young Hak , Konishi Eiichi , Takayama Koichi 
Published: October 2022

Abstract
Purpose
Growth differentiation factor 15 (GDF-15) is one of the key cachexia-inducible factors. Clinical trials on therapies targeting GDF-15 for cancer and cancer cachexia are underway. While the role of circulating GDF-15 in cachexia has been clarified, the effects of GDF-15 expression within cancer cells remain to be fully elucidated. Hence, the objective of this study was to investigate the expression of GDF-15 in advanced lung cancer tissues and to understand its role in cachexia.
Methods
We retrospectively examined the expression level of full-length GDF-15 in advanced non-small cell lung cancer tissues and analyzed the relationship between the staining intensity and clinical data in 53 samples.
Results
We found that 52.8% of the total samples were GDF-15 positive, and the GDF-15 expression significantly correlated with better nutrition and a low inflammation state. It did not correlate with the existence of cancer cachexia and overall survival. Patients with GDF-15-positive expression tended to have better survival than those with GDF-15-negative expression.
Conclusion
Thus, tumor GDF-15 and its family of cytokines are diverse. It is necessary to characterize their role in cancer cachexia and identify whether GDF-15 can be a predictive biomarker for cachexia in non-small cell lung cancer, which could lead to better therapeutic strategies targeting GDF-15.
Differential behavior of bone turnover markers in women with bone metastatic breast cancer or osteoporosis
Authors: Soares Ana Laura , Vieira José Gilberto Henrique , Moreira Linda Denise Fernandes , Simon Sérgio Daniel , Gebrim Luiz Henrique , Nazário Afonso Celso Pinto , Silva André Gonçalves da , Castro Marise Lazaretti 
Published: October 2022

Abstract
Background: This study was designed to describe bone turnover marker (BTM) profiles in women.
Patients and methods: In all, 197 patients (age 61 [36-90] years) comprising five groups were studied: osteoporosis with (OPBP+) or without (OPBP-) bisphosphonate use; bone metastatic breast cancer with (BMBP+) or without (BMBP-) BP use; and healthy controls without BP (CBP-) use. Procollagen type 1 amino-terminal propeptide (P1NP) and carboxy-terminal telopeptide of type 1 collagen (CTX) were analyzed.
Results: The medians [25%-75%; ng/mL] for P1NP were as follows: BMBP- (236.95 [165.0-328.0]) > CBP- (47.25 [33.5-63.7]) = OPBP- (50.9 [37.4-63.9]) > BMBP+(26.9 [11.8-46.3]) = OPBP+ (19.5 [12.6-27.3]).
The medians [25% -75%; ng/mL] for CTX were as follows: BMBP- (0.567 [0.457-0.803]) = OPBP- (0.360 [0.318-0.650]) > CBP- (0.297 [0.203-0.402]) > BMBP+(0.101 [0.052-0.202]) = OPBP+(0.141 [0.047-0.186]).
Conclusion: P1NP > 145 ng/mL completely differentiated those with BMs. CTX < 0.200 ng/mL differentiated those using BPs.
A Prognostic Model Based on Necroptosis-Related Genes for Prognosis and Therapy in Bladder Cancer
Authors: Wang Zeyi , Huang Zhengnan , Cao Xiangqian , Zhang Fang , Cai Jinming , Tang Pengfei , Yang Chenkai , Li Shengzhou , Yu Dong , Yan Yilin , Shen Bing 
Published: October 2022

Abstract

Bladder cancer, one of the most prevalent malignant cancers, has high rate of recurrence and metastasis. Owing to genomic instability and high-level heterogeneity of bladder cancer, chemotherapy and immunotherapy drugs sensitivity and lack of prognostic markers, the prognosis of bladder cancer is unclear. Necroptosis is a programmed modality of necrotic cell death in a caspase-independent form. Despite the fact that necroptosis plays a critical role in tumor growth, cancer metastasis, and cancer patient prognosis, necroptosis-related gene sets have rarely been studied in ladder cancer. As a result, the development of new necroptosis-related prognostic indicators for bladder cancer patients is critical. Herein, we assessed the necroptosis landscape of bladder cancer patients from The Cancer Genome Atlas database and classified them into two unique necroptosis-related patterns, using the consensus clustering. Then, using five prognosis-related genes, we constructed a prognostic model (risk score), which contained 5 genes (ANXA1, DOK7, FKBP10, MAP1B and SPOCD1). And a nomogram model was also developed to offer the clinic with a more useful prognostic indicator. We found that risk score was significantly associated with clinicopathological characteristics, TIME, and tumor mutation burden in patients with bladder cancer. Moreover, risk score was a valid guide for immunotherapy, chemotherapy, and targeted drugs. In our study, DOK7 was chose to further verify our prognosis model, and functional assays indicated that knockdown the expression of DOK7 could prompt bladder cancer proliferation and migration. Our work demonstrated the potential role of prognostic model based on necroptosis genes in the prognosis, immune landscape and response efficacy of immunotherapy of bladder cancer.
Radiological evaluation of the prevalence of bone loss, tooth wear, periodontal ligament widening, and the prevalence of mandibular torus
Authors: Nirit Tagger-Green , Nemcovski Carlos , Berg Yarden Sagi , Fridenberg Naama , Kolerman Roni 
Published: October 2022

Abstract
Objective: The aim of the study was to quantify the relationship between excessive occlusal forces reflected by tooth wear, Periodontal ligament space widening (PDLw), TM presence, and radiographic loss of bone support. A secondary aim was to evaluate the correlation of screening specific teeth instead of the entire dentition.
Materials and Methods: 1950 Sets of full mouth radiographic surveys were retrospectively analyzed. Radiologic quantification of loss of bone support was performed using the "Schei's ruler" technique. Tooth wear evaluation was classified according to enamel wear in the incisal area, PDL widening was considered when over 0.2mm was measured, and the presence of mandibular torus was inspected.
Results: After collecting data from the first 400 radiographs, the correlation between specific teeth and the whole dentition was evaluated. Teeth 41 and 33 showed the best correlation scores to all examined teeth. Tooth wear and loss of bone support were significantly age-related (p<0.001). There was a statistically significant correlation between tooth wear and bone support loss (p<0.001). The odds ratio of having bone loss when tooth wear was present was 2.767. The odds ratio of having bone loss when PDLw was present was 2.585.
Conclusion: The present study found a statistically significant correlation between radiographic tooth wear, PDLw, and loss of bone support.
Clinical Relevance: The high correlation between tooth wear and radiographic bone loss helps the clinician better understand the etiology of periodontal disease. Prevention and treatment of the disease are multifactorial.
A 90-day subchronic oral toxicity assessment of cry11Ba protein expressed in genetically modified Chlamydomonas reinhardtii by using Han Wistar rats.
Authors: Khan Hamza , Khan Mohsin Ahmad , Ahmed Nadeem , Tawakkal Farah , Amjad Fareeha , Pervaiz Rabbia 
Published: October 2022

Abstract
Mosquito-borne diseases are a persistent risk to humanity all over the world. A genetically modified algae (Chlamydomonas reinhardtii) producing mosquito larvicidal cry11Ba protein was constructed in Biopharmaceutical Laboratories of CEMB, University of Punjab, Pakistan. The current study aims at the biosafety assessment of this transgenic algae in Han Wistar rats for a 90-days trial period. Thirty male Han Wistar rats were used in the experiment, divided into three groups which were given transgenic algae mixed water (at 1x105cell mL− 1 concentration), non-transgenic algae mixed water (at 1x105cell mL− 1 concentration), and simple water without algae respectively for 90-days. Han Wistar rats were regularly clinically observed for any mortality, allergenicity, moribundity, water consumption, feed consumption, body weight, hematology, serum chemistry (LFT, RFT, serum electrolytes), urine analysis, molecular analysis, necropsy analysis (organs morphology, organ weight, and histopathology of vital organs) and found no substantial difference among any of the groups. In conclusion, there was no adverse effect found on the overall health of male Han Wistar rat feeding on transgenic algae (Chlamydomonas reinhardtii) producing the cry11Ba protein.
The Benefits of Sacubitril/Valsartan Administration in Patients with ST-Segment Elevation Myocardial Infarction: A Meta-Analysis
Authors: Ge Rongguang , Gong Yuan , Huang Mingxuan , Lu Siyu , Xiu Lu , Li Linwei , Huang Wenqi , Chang Jie 
Published: October 2022

AbstractPurpose This meta-analysis aimed to clarify the clinical effects and safety of Sacubitril/Valsartan (Sac/Val) administration in patients with ST-segment elevation myocardial infarction (STEMI). Methods This analysis was registered with PROSPERO (CRD42022364008). Researchers performed a systematic search of English and Chinese databases to identify available randomized controlled trials from inception to September 30, 2022. The experimental group was given Sac/Val, while the control group was given conventional angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB). Study screening and data extraction were conducted by two independent researchers. Cochrane Handbook 6.3 was used for quality evaluation and bias assessment. Risk ratio (RR) and 95% confidential interval (95% CI) were adopted for enumeration data. Mean difference (MD) and 95% CI were adopted for measurement data. Results A total of 7 studies with 964 patients were included in the meta-analysis. The outcomes of clinical effects, including rehospitalization for heart failure (HF), left ventricular ejection fraction (LVEF), major adverse cardiac events (MACEs), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) concentration at 24 weeks, were respectively RR = 0.45 (95% CI: 0.35 to 0.66), MD = 3.44 (95% CI: 2.66 to 4.22), RR = 0.50 (95% CI: 0.40 to 0.62), and MD=-155.55 (95% CI: -226.35 to -84.75). Meanwhile, the outcomes of adverse side effects, including hypotension, hyperkalemia, cough, and worsening renal function, were respectively RR = 1.81 (95% CI: 1.09 to 3.02), RR = 0.58 (95% CI: 0.17 to 1.93), RR = 0.84 (95% CI: 0.26 to 2.72), and RR = 0.55 (95% CI: 0.23 to 1.33). Conclusions Compared with conventional ACEI/ARB, Sac/Val was found to improve cardiac function, decrease the risk of HF, and reduce MACEs incidence in patients after STEMI. However, in terms of adverse side effects, Sac/Val did not show a significant advantage over ACEI/ARB.
Identification and validation of two hub genes involved in membranous nephropathy based on machine learning
Authors: Pan Yu-Biao , Ye Hua-Mao , Jiang Zuan-Hong , Chen De-Jun , Teng Ya , Guan Chen-An 
Published: October 2022

Abstract
Background: Membranous nephropathy (MN) is an autoimmune disease. It is an important cause of end-stage renal disease in primary glomerulonephritis. Significant breakthroughs in its diagnosis have been made in previous studies, however, the pathogenesis of MN has still remained elusive. In recent years, bioinformatics has provided new research strategies to investigate the mechanisms of kidney disease. This study aimed to explore potential biomarkers of MN through bioinformatics analysis.
Methods: Differentially expressed genes (DEGs) were identified by performing a differential expression analysis with the "limma" R package, and then, the weighted gene co-expression network analysis (WGCNA) was applied to obtain the most MN-related genes. After intersecting these genes, the least absolute shrinkage and selection operator (LASSO) and the support vector machine-recursive feature elimination (SVM-RFE) algorithms were utilized to identify hub genes. To assess the diagnostic value of hub genes, the receiver operating characteristic (ROC) curve analysis was performed. Finally, the relationship between hub genes and the immune microenvironment (IME) was analyzed.
Results: The differential expression analysis yielded 1,466 DEGs, and using the WGCNA, 442 genes, which were the most MN-related genes, were obtained. From the intersection of these genes, 130 genes were identified. Subsequently, two hub genes (ECM1 and ATP8B1) were detected by the LASSO and SVM-REF algorithms. It was found that they were associated with components of the IME (natural killer T cells, gamma delta T cells, macrophages, etc.).
Conclusion: Two hub genes (ECM1 and ATP8B1) were identified by machine learning, and their diagnostic value was evaluated. It was revealed that these two genes were associated with the components of the IME. Our findings may provide new ideas for developing new biomarkers for MN.
Feasibility of cardiovascular magnetic resonance imaging at 5T in comparison to 3T
Authors: Lan Lan , Hu Huijuan , Sun Wenbo , Sun Rongqing , Ling Gonghao , Du Tingyi , Li Xuan , Yuan Jianmin , Xing Yaowen , Song Xiaopeng , Xu Haibo 
Published: October 2022

Abstract
Purpose
Due to safety and technical issues, 7T MRI has not been approved by the FDA for cardiovascular magnetic resonance imaging (CMR), however, 5T has been proved to be safe for whole body imaging. In this study, we investigated the feasibility and performance of CMR at a 5T whole body system, which may face less technical challenges than 7T.
Methods
Seventeen healthy volunteers and three patients were recruited and underwent CMR scans on both 5T and 3T MR scanners. Cine images of short-axis views as well as three standard long-axis views were acquired at 3T using balanced steady state free precession sequence, and at 5T using fast gradient echo sequence respectively. Dark blood imaging was repeated in a slice of midventricular short-axis view at 5T and 3T as well. Image quality and artifact level were estimated on a 5-point scale and compared between different field strengths. Quantifications of functional parameters and mass of left ventricle (LV) were compared between the two field strengths.
Results
The overall image acquired at 5T was comparable to that obtained at 3T, achieving image quality scores of 4.61±0.39 and 4.59±0.56 for dark blood sequences, 4.60±0.50 for FGRE sequence. Artifact level scores for black blood sequences were 4.72±0.46 and4.65±0.54, while FGRE sequence achieved 4.65±0.49. Bland-Altman analysis demonstrated that functional parameters and mass of LV derived from 5T were in good agreement with the 3T counterparts.
Conclusion
This study confirmed the feasibility of CMR imaging at 5T MR system.
Behaviour change interventions based on automated and tailored communication systems to improve health outcomes in adult chronic patients: a systematic review
Authors: Martins Ana , Londral Ana , Lapão Luís Velez 
Published: October 2022

Abstract
Background: Behaviour change digital-assisted interventions can be a low-cost solution to enduring healthy behaviours. Understanding how to automate and tailor such interventions to patients’ needs can improve health outcomes. This study aims at reviewing the evidence of the use of such interventions in a clinical setting in adult chronic patients.
Methods: This study followed the PRISMA guidelines for comprehensive search, appraisal, and synthesis of research evidence. A literature search was performed to find studies published between 1 January 2017 and 26 March 2022 in peer-reviewed journals and written in English. Behaviour change interventions in adult chronic patients with automated and tailored communication systems were considered.
Results: Seven quantitative studies (6 RCT and a pilot RCT) with sample sizes from 54 to 506 participants and timeframes varying from 4 to 30 weeks met the eligibility criteria. Populations comprehended patients with cardiovascular disease, chronic kidney disease, diabetes, or osteoarthritis. Communication channels included SMS, email, and voice records. Rule-based models were used to automate content in all studies and bidirectional communication was used in four of them. Content and service delivery were tailored given the patient’s goals, needs, functional ability, activity, personal characteristics, and communication channel preferred. Most data management platforms used were developed in prior studies. Interventions were designed based on 9 different behaviour change models. There was no evidence of improvements in clinical outcomes after the intervention. Some studies showed improvements in self-efficacy (n=1), the use of dietary data info (n=1), physical activity (n=2), social support (n=1), and quality of life (n=1). The risk of bias analysis revealed that one study had moderate risk and the remaining had low risk.
Conclusion: This study supports researchers that are designing digital behaviour change interventions by putting in evidence the intervention’s features needed to be considered during this step. It revealed that the most efficient solutions were tailored to patients’ needs and disease stages and that further research on the mechanisms of change and content automation needs to be done.
A prospective comparative study of 68Ga-RM26 and 68Ga-PSMA-617 PET/CT imaging in suspicious prostate cancer
Authors: Gao Xiaomei , Tang Yongxiang , Chen Minfeng , Li Jian , Yin Hongling , Gan Yu , Zu Xiongbin , Cai Yi , Hu Shuo 
Published: October 2022

Abstract
Purpose Prostate-specific membrane antigen (PSMA) -based PET/CT imaging showed limitations in the diagnosis of prostate cancers (PCa). We recruited 207 participants with suspicious PCa to perform PET/CT imaging with radiolabeled gastrin-releasing peptide receptor (GRPR) antagonist, 68Ga-RM26, and compare with 68Ga-PSMA-617 and histopathology.
Methods Participants with suspicious PCa were subjected to 68Ga-RM26 and 68Ga-PSMA-617 PET/CT. PET/CT imaging was compared using pathologic specimens as a reference standard.
Results Of the 207 participants analyzed, 125 had cancer, and 82 were diagnosed with benign prostatic hyperplasia (BPH). The sensitivity and specificity of 68Ga-RM26 and 68Ga-PSMA-617 PET/CT imaging differed significantly for detecting clinically significant prostate cancer (csPCa). The area under the ROC curve (AUC) was 0.54 for 68Ga-RM26 PET/CT and 0.91 for 68Ga-PSMA-617 PET/CT detecting PCa. For csPCa imaging, the AUCs were 0.51 vs. 0.93, respectively. However, 68Ga-RM26 PET/CT imaging had higher sensitivity for PCa with Gleason Score (GS) =6 (p=0.03) than 68Ga-PSMA-617 PET/CT but poor specificity (20.73%). In the group with PSA <10 ng/mL, the sensitivity, specificity, and AUC of 68Ga-RM26 PET/CT were lower than 68Ga-PSMA-617 PET/CT (60.00% vs. 80.30%, p=0.12, 23.26% vs. 88.37%, p=0.000, and 0.524 vs. 0.822, p=0.000, respectively). 68Ga-RM26 PET/CT exhibited a significantly higher SUVmax in specimens with GS=6 (p=0.04) and in the low-risk group (p=0.01), and its uptake did not increase with the PSA level, GS, or clinical stage.
Conclusion This prospective study provided evidence for superior accuracy of 68Ga-PSMA-617 PET/CT over 68Ga-RM26 PET/CT in the detection of csPCa. And 68Ga-RM26 PET/CT showed an advantage for imaging of low-risk PCa.
Nutcracker syndrome: an uncommon cause of frank hematuria: a case report
Authors: Jelleli Nejmeddine , Jemaa Mohamed Ben , Sakly Aymen , Jelleli Bilel , Zakhama Walid , Chouaya Ines , Sidhom Wael , Chouaya Sabrine , Binous Mohamed Yassine 
Published: October 2022

Abstract
A 28-year-old young woman was referred to our urology consultation for hematuria associated with chronic left flank pain. The physical examination revealed tenderness on palpation of the left lumbar fossa (Visual analogue scale (VAS) = 5/10). Urinalysis confirmed the macroscopic hematuria. Abdomen computed tomography (CT) angiography revealed compressive trapping of the left renal vein (LRV) at the level of the aorto-mesenteric clamp. The diagnosis of nutcracker syndrome (NCS) in its anterior variant was made. The decision of our urology team in collaboration with the vascular surgeons was to opt for conservative treatment. During the last clinical follow-up dating back to18 months after the diagnosis, the patient had no macroscopic hematuria. She reported reduction in flank pain intensity (VAS= 2/10).
Phenanthroimidazole as Molecularly Engineered Switch for Efficient and Highly Long-lived Light-Emitting Electrochemical Cell.
Authors: Bideh Babak Nemati , Moghadam Majid , Sousaraei Ahmad , Arani Behnoosh Shahpoori 
Published: October 2022

Abstract
Although, light-emitting electrochemical cells (LECs) based on Ir(III) complexes owing to the superior advantages exhibit high potential for display and lighting applications, they still suffer from relatively low stability and sluggish response time. To mitigate this challenge, herein, a series of Ir(III) complexes based on phenanthroimidazole (PI) as ancillary ligand were functionalized to achieve efficient, highly stable yellow to orange LEC devices with fast response. These complexes exhibit appropriate electrochemical stability and significant suppression of concentration quenching in the thin films compare to archetype complex. Concerning, the fabricated LECs showed remarkable long device lifetime over 1400 and 2100 hours and EQE of 2 and 3% for yellow and orange-LECs, respectively, in which obtained t1/2 for yellow LEC is among the highest value for cationic iridium (III) complexes based yellow-LECs reported so far. Subsequently, incorporation of ionic tethered functional group on PI, improved the mobility of emissive layer, reducing the device turn-on time around 75–88%. This study represents facile functionalization and characterization of PI ligand and its potential application in optoelectronic devices (OLED).
A Probabilistic Model for Annual Maximum Peak Ground Motion Based on Shifted Lognormal Distribution
Authors: Ge Fang-Wen , Zhao Yan-Gang , Zhang Haizhong 
Published: October 2022

Abstract
Continuous seismic hazard curves can be used to flexibly determine earthquake intensity with an arbitrary annual exceedance probability for probabilistic structural seismic design. To obtain an explicit expression of the continuous curve based on several given earthquake intensities corresponding to different hazard levels from probabilistic seismic hazard analysis (PSHA), a model of the annual maximum peak ground motion based on the Fréchet distribution has been widely adopted over the past decades. However, recent studies showed that the mathematical limitations of statistic estimation and poor performance in the upper tail region of the Fréchet model are unfavorable for use in reliability-based studies, particularly for vital structures. In this study, a new probabilistic model for the annual maximum peak ground motion is proposed based on a shifted lognormal distribution. The performance of the proposed model was statistically validated by investigating the global fitting effect, accuracy of the upper tail region, and appropriateness of the estimated statistics based on a long-term Japanese earthquake catalogue. The application of the proposed model to generate continuous seismic hazard curves by fitting different types of the analytical PSHA data is illustrated using two numerical examples.