bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spruce (Picea abies L.) planting leads post-clearcut bird assemblages to a novel successional pathway—a comparative study in hemiboreal mixed forests
Authors: Pass Eliisa , Kont Raido , Lõhmus Asko 
Published: April 2022

AbstractKey messageBird assemblages in planted spruce (Picea abiesL.) forests were compositionally distinct from those in naturally regenerated mixed stands. Despite rapid stand development on productive soils, even-aged silviculture cannot host most old-forest species. However, their habitat quality could be supported in landscape mosaics with retention forestry and set-asides.ContextThe most productive forest lands have naturally the richest bird assemblages but tend to be also most intensively managed. Sustainable solutions to this conflict are unclear.AimTo assess bird assemblages and their successional dynamics in planted Norway spruce (Picea abiesL.) stands compared to naturally developing stands.MethodsWe mapped breeding bird assemblages in forty 5-ha plots on highly productive soils in Estonia. The plots included sets of naturally regenerated and planted stands, and (as successional endpoints) clear-cuts and old stands.ResultsPlanted stands had fewer bird species and pairs than naturally regenerated stands; the latter having a species composition resembling late-successional deciduous-dominated stands. Importantly, the species composition in mature spruce plantations converged toward the composition observed in late-successional conifer-dominated stands. Downed dead wood, stand age, deciduous trees, and stock density were the most significant stand characteristics shaping bird assemblages.ConclusionThe habitat value of established spruce plantations can be primarily improved by allowing for some deciduous trees and gaps with deciduous undergrowth. At the landscape scale, the bird diversity of even-aged systems would be enhanced by multi-scale applications of retention forestry—from retention trees to old-growth set-asides.
Long-term Weight Change and its Temporal Relation to Later-life Dementia in the Health and Retirement Study
Authors: Shen Jie , Chen Hui , Zhou Tianjing , Zhang Simei , Huang Liyan , Lv Xiaozhen , Ma Yuan , Zheng Yan , Yuan Changzheng 
Published: April 2022

Abstract

Context
Weight loss among middle-aged and older adults has been associated with a higher risk of subsequent dementia. However, most studies have limited follow-up durations or suboptimal control for the potential influence of physical frailty (PF).

Objective
Our study aimed to investigate the long-term and temporal relations of weight change to risk of dementia among middle-aged and older adults in the United States.

Methods
A total of 5985 participants aged 65 years and older were included from the Health and Retirement Study. History of long-term weight change was calculated using 9 repeated body mass index measurements during 1992-2008. We then followed participants’ dementia status from 2008 to 2018. Multivariable Cox proportional hazard models were used.

Results
During the study follow-up period (mean = 7.54 years), a total of 682 (11.40%) dementia cases were documented. After adjustment for basic demographic and lifestyle factors, participants with weight loss (median: −0.23 kg/m2 per year) were at a significantly higher risk of dementia (HR = 1.60; 95% CI, 1.33, 1.92), compared with the stable weight group (median: 0.11 kg/m2 per year). This association was attenuated but remained strong and significant after further adjustment for PF (HR = 1.57; 95% CI, 1.30, 1.89). Significant association was observed for weight loss assessed approximately 14 to 18 years preceding dementia diagnosis (HR = 1.30; 95% CI, 1.07, 1.58), and was consistent closer to diagnosis.

Conclusion
Both recent and remote weight loss were associated with a higher risk of later-life dementia among middle-aged and older adults independent of PF status.
Avian auditory hair cell regeneration is accompanied by JAK/STAT-dependent expression of immune-related genes in supporting cells
Authors: Janesick Amanda S. , Scheibinger Mirko , Benkafadar Nesrine , Kirti Sakin , Heller Stefan 
Published: April 2022

ABSTRACT
The avian hearing organ is the basilar papilla that, in sharp contrast to the mammalian cochlea, can regenerate sensory hair cells and thereby recover from deafness within weeks. The mechanisms that trigger, sustain and terminate the regenerative response in vivo are largely unknown. Here, we profile the changes in gene expression in the chicken basilar papilla after aminoglycoside antibiotic-induced hair cell loss using RNA-sequencing. We identified changes in gene expression of a group of immune-related genes and confirmed with single-cell RNA-sequencing that these changes occur in supporting cells. In situ hybridization was used to further validate these findings. We determined that the JAK/STAT signaling pathway is essential for upregulation of the damage-response genes in supporting cells during the second day after induction of hair cell loss. Four days after ototoxic damage, we identified newly regenerated, nascent auditory hair cells that express genes linked to termination of the JAK/STAT signaling response. The robust, transient expression of immune-related genes in supporting cells suggests a potential functional involvement of JAK/STAT signaling in sensory hair cell regeneration.
The immune environment of the mammary gland fluctuates during post-lactational regression and correlates with tumour growth rate
Authors: Hitchcock Jessica , Hughes Katherine , Pensa Sara , Lloyd-Lewis Bethan , Watson Christine J. 
Published: April 2022

ABSTRACT
Post-lactational mammary gland regression encompasses extensive programmed cell death and removal of milk-producing epithelial cells, breakdown of extracellular matrix components and redifferentiation of stromal adipocytes. This highly regulated involution process is associated with a transient increased risk of breast cancer in women. Using a syngeneic tumour model, we show that tumour growth is significantly altered depending on the stage of involution at which tumour cells are implanted. Tumour cells injected at day 3 involution grew faster than those in nulliparous mice, whereas tumours initiated at day 6 involution grew significantly slower. These differences in tumour progression correlate with distinct changes in innate immune cells, in particular among F4/80-expressing macrophages and among TCRδ+ unconventional T cells. Breast cancer post-pregnancy risk is exacerbated in older first-time mothers and, in our model, initial tumour growth is moderately faster in aged mice compared with young mice. Our results have implications for breast cancer risk and the use of anti-inflammatory therapeutics for postpartum breast cancers.
Natural killer cells act as an extrinsic barrier for in vivo reprogramming
Authors: Melendez Elena , Chondronasiou Dafni , Mosteiro Lluc , Martínez de Villarreal Jaime , Fernández-Alfara Marcos , Lynch Cian J. , Grimm Dirk , Real Francisco X. , Alcamí José , Climent Núria , Pietrocola Federico , Serrano Manuel 
Published: April 2022

ABSTRACT
The ectopic expression of the transcription factors OCT4, SOX2, KLF4 and MYC (OSKM) enables reprogramming of differentiated cells into pluripotent embryonic stem cells. Methods based on partial and reversible in vivo reprogramming are a promising strategy for tissue regeneration and rejuvenation. However, little is known about the barriers that impair reprogramming in an in vivo context. We report that natural killer (NK) cells significantly limit reprogramming, both in vitro and in vivo. Cells and tissues in the intermediate states of reprogramming upregulate the expression of NK-activating ligands, such as MULT1 and ICAM1. NK cells recognize and kill partially reprogrammed cells in a degranulation-dependent manner. Importantly, in vivo partial reprogramming is strongly reduced by adoptive transfer of NK cells, whereas it is significantly increased by their depletion. Notably, in the absence of NK cells, the pancreatic organoids derived from OSKM-expressing mice are remarkably large, suggesting that ablating NK surveillance favours the acquisition of progenitor-like properties. We conclude that NK cells pose an important barrier for in vivo reprogramming, and speculate that this concept may apply to other contexts of transient cellular plasticity.
Effect of prenatal glucocorticoids and thyroid hormones on developmental plasticity of mitochondrial aerobic metabolism, growth and survival: an experimental test in wild great tits
Authors: Cossin-Sevrin Nina , Hsu Bin-Yan , Marciau Coline , Viblanc Vincent A. , Ruuskanen Suvi , Stier Antoine 
Published: April 2022

ABSTRACT
Developmental plasticity is partly mediated by transgenerational effects, including those mediated by the maternal endocrine system. Glucocorticoid and thyroid hormones may play central roles in developmental programming through their action on metabolism and growth. However, the mechanisms by which they affect growth and development remain understudied. One hypothesis is that maternal hormones directly affect the production and availability of energy-carrying molecules (e.g. ATP) by their action on mitochondrial function. To test this hypothesis, we experimentally increased glucocorticoid and thyroid hormones in wild great tit eggs (Parus major) to investigate their impact on offspring mitochondrial aerobic metabolism (measured in blood cells), and subsequent growth and survival. We show that prenatal glucocorticoid supplementation affected offspring cellular aerobic metabolism by decreasing mitochondrial density, maximal mitochondrial respiration and oxidative phosphorylation, while increasing the proportion of the maximum capacity being used under endogenous conditions. Prenatal glucocorticoid supplementation only had mild effects on offspring body mass, size and condition during the rearing period, but led to a sex-specific (females only) decrease in body mass a few months after fledging. Contrary to our expectations, thyroid hormone supplementation did not affect offspring growth or mitochondrial metabolism. Recapture probability as juveniles or adults was not significantly affected by prenatal hormonal treatment. Our results demonstrate that prenatal glucocorticoids can affect post-natal mitochondrial density and aerobic metabolism. The weak effects on growth and apparent survival suggest that nestlings were mostly able to compensate for the transient decrease in mitochondrial aerobic metabolism induced by prenatal glucocorticoids.
Cross-fringe piston sensor for segmented optics
Authors: Sato Seichi , Mizutani Tadahito 
Published: April 2022

This paper presents a simple slit-mask-based sensing scheme that can be applied to a robust piston error sensor for segmented optical systems, even in space. Utilizing crossed fringes generated from multiple double-slit pairs, the sensor simultaneously detects multiple points in a segmented optical system in one shot. The experiments tested six pistons of a single-ring segmented mirror. The results showed measurement stability better than 15 nm in the range of 0–5 µm and absolute accuracy of

λ

/

20

. The theoretical perspective of applicability for larger piston measurement and N-ring segmented systems is also discussed, with simulation results for a two-ring system presented.
Spreading evolution of a linear phase-locked hollow beam array in atmospheric turbulence
Authors: Zhao Jie , Dong Rui , Li Yaping , Zou Jiayi , Qiao Chunhong , Lu Lu 
Published: April 2022

Based on the generalized Huygens–Fresnel principle, propagation characteristics of a linear phase-locked hollow beam (PLHB) array propagating through atmospheric turbulence are investigated. Analytical expressions of the average intensity and mean-squared beam width of the PLHB array are derived. It is shown that propagation properties of a PLHB array are related to the initial beam parameters (i.e., number of beamlets, size of beam waist, range of wavelength) of the PLHB array and the strength of turbulence. These findings may provide potential applications in free-space optical communications, lidar, remote sensing, and related fields.
Prevalence and associated factors of treatment failure among children on ART in Ethiopia: A systematic review and meta-analysis
Authors: Gelaw Belete , Dessalegn Lemma , Alem Eyasu , Tekalign Tiwabwork , Lankirew Tadele , Eshetu Kirubel , Marew Chalie , Chekole Bogale , Kassaw Amare 
Published: April 2022

Background
As the use of antiretroviral therapy (ART) increases, the issue of treatment failure is still a global challenge, particularly in a resource limited settings including Ethiopia. The results of former studies in Ethiopia were highly variable and inconsistent across studies. Thus, this systematic review and meta-analysis intended to provide the pooled estimation of treatment failure and associated factors among children on antiretroviral therapy.

Methods
We searched international databases (i.e., PubMed, Google Scholar, Web of Science, Ethiopian Universities’ online repository library, Scopus, and the Cochrane Library) during the period of February 30 to April 7, 2021. All identified observational studies reporting the proportion of treatment failure among HIV positive children in Ethiopia were included. Heterogeneity of the studies was checked using I2 test and Cochrane Q test statistics. We run Begg’s regression test to assess publication bias. A random-effects meta-analysis model was performed to estimate the pooled prevalence of treatment failure.

Results
The estimated pooled prevalence of treatment failure among children in Ethiopia was 12.34 (95%CI: 8.59, 16.10). Subgroup analysis of this review showed that the highest prevalence was observed in Addis Ababa (15.92%), followed by Oromia region (14.47%). Poor ART adherence (AOR = 2.53, CI: 2.03, 4.97), advanced WHO clinical staging (AOR = 1.66, CI: 1.24, 3.21), and opportunistic infections (AOR = 2.64 CI: 2.19, 4.31 were found to be significantly associated factors with childhood treatment failure.

Conclusions
This study revealed that treatment failure among children on ART was high in Ethiopia. Poor ART adherence, advanced WHO clinical staging, opportunistic infections, and low level of CD4 cell counts increased the risk of treatment failure.
Public opinion about the UK government during COVID-19 and implications for public health: A topic modeling analysis of open-ended survey response data
Authors: Wright Liam , Burton Alexandra , McKinlay Alison , Steptoe Andrew , Fancourt Daisy 
Published: April 2022

Background
Confidence in the central UK Government has declined since the beginning of the COVID-19 pandemic, and while this may be linked to specific government actions to curb the spread of the virus, understanding is still incomplete. Examining public opinion is important, as research suggests that low confidence in government increases the extent of non-compliance with infection-dampening rules (for instance, social distancing); however, the detailed reasons for this association are still unclear.

Methods
To understand public opinion on the central UK government during the first phase of the COVID-19 pandemic, we used structural topic modeling, a text mining technique, to extract themes from over 4000 free-text survey responses, collected between 14 October and 26 November 2020.

Results
We identified eleven topics, among which were topics related to perceived government corruption and cronyism, complaints about inconsistency in rules and messaging, lack of clear planning, and lack of openness and transparency. Participants reported that elements of the government’s approach had made it difficult to comply with guidelines (e.g., changing rules) or were having impacts on mental wellbeing (e.g., inability to plan for the future).

Conclusions
Results suggested that consistent, transparent communication and messaging from the government is critical to improving compliance with measures to contain the virus, as well as protecting mental health during health emergencies.
Association between serum β2-microglobulin and mortality in Japanese peritoneal dialysis patients: A cohort study
Authors: Maruyama Yukio , Nakayama Masaaki , Abe Masanori , Yokoo Takashi , Minakuchi Jun , Nitta Kosaku 
Published: April 2022

Background
Higher serum β2-microglobulin (B2M) concentrations are associated with higher mortality in the general population, non-dialyzed chronic kidney disease patients and patients receiving hemodialysis (HD). However, this relationship among patients on peritoneal dialysis (PD) has not been validated.

Methods
We collected baseline data for 3,011 prevalent PD patients from a nationwide dialysis registry in Japan at the end of 2010. Clinical outcomes for 9 years were then evaluated using the registry at the end of 2011 to 2019. All-cause and cardiovascular (CV) mortality was assessed using Cox regression analysis and competing-risks regression analysis, respectively. We used multiple imputation to deal with missing covariate data.

Results
During a median follow-up of 87 months, 2,054 patients transferred to combined therapy with PD and HD or HD directly. A total of 3,011 patients, 1,235 (41.0%) died, including 437 patients (14.5%) from CV causes. Among them, 612 patients died after transfer to other dialysis modalities. Univariate analyses revealed no significant association between serum B2M and mortality, whereas higher serum B2M was independently associated with both all-cause and CV mortalities in adjusted models. However, the significant association between serum B2M and CV mortality disappeared in analysis treating serum B2M as a categorical variable. The effect of serum B2M on all-cause mortality was significantly higher among patients with higher urinary volume and a significant interaction was evident.

Conclusions
Using a large-scale registry, we found that serum B2M contributes tenuously but significantly to worse outcome and residual kidney function significantly affects this relationship. On the contrary, serum B2M per se had no predictive value for patient outcome in prevalent PD patients.
Risky sexual practice and associated factors among people living with HIV/AIDS receiving antiretroviral therapy in Ethiopia: Systematic review and meta-analysis
Authors: Hareru Habtamu Endashaw , Kaso Abdene Weya , Ashuro Zemachu , Mareg Moges 
Published: April 2022

Background
The risky sexual behavior of people living with HIV/AIDS (PLWHA) may impose a risk of transmitting the disease to their partners and increase Human Immunodeficiency Virus (HIV) co-infection. This systematic review and meta-analysis aimed to determine the pooled prevalence of risky sexual behavior and associated factors among PLWHA receiving [Antiretroviral Therapy (ART)] in Ethiopia.

Methods
To identify both published and unpublished research articles, systematic searches were performed in PubMed, HINARI, Medline, Science Direct, and Google Scholar databases. The review was carried out following the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guideline. Cross-sectional studies reporting the prevalence of risky sexual practice and its associated factors among PLWHA receiving ART in Ethiopia were included. Two authors independently extracted all necessary data using a standardized data extraction format prepared in Microsoft Excel and exported to STATA version 14 statistical software for further analyses. The Cochrane Q test statistics and I2 test were used to assess the heterogeneity of the studies. Since the included studies exhibited considerable heterogeneity, the random-effects meta-analysis model was computed to estimate the pooled prevalence of risky sexual practice which was determined by dividing the total number of PLWHA with risky sexual practice practices by the total number of PLWHA on ART in the study and multiplied by 100. Furthermore, pooled odds ratio (OR) with 95% confidence interval (CI) was determined for the association between determinant factors and risky sexual practice.

Result
In this study, 2351 articles were identified from different databases, and fifteen articles were selected for final systematic review and meta-analysis. In Ethiopia, the pooled prevalence of risky sexual practices was 43.56% (95% confidence interval (CI):35.51, 51.62). Discussion about safe sex with sexual partner/s [AOR = 0.26, 95% CI: 0.08, 0.92] and having multiple sexual partners [AOR = 1.90, 95% CI: 0.53, 6.84] were factors significantly associated with risky sexual practice in Ethiopia.

Conclusion
A significant proportion of respondents engaged in risky sexual practices. Multiple sexual partners and a lack of discussion about safe sex are linked to a higher prevalence of the risky sexual practice in Ethiopia. It is critical to raise awareness about safe sexual practices during health education and counselling services and to encourage clients to freely discuss safer sex practices with their sexual partner/s at their antiretroviral therapy (ART) appointments as part of their follow-up care.

Protocol registration
The protocol for this systematic review and meta-analysis was registered at PROSPERO (record ID = CRD42021274600, 25 September 2021).
Knowledge and practices surrounding malaria and LLIN use among Arab, Dazagada and Fulani pastoral nomads in Chad
Authors: Moukénet Azoukalné , Richardson Sol , Moundiné Kebféné , Laoukolé Jean , Ngarasta Ngarkodje , Seck Ibrahima 
Published: April 2022

Background
Chadian pastoral nomads are highly exposed to malaria due to their lifestyle and their mobility between various endemic areas. To inform strategies to reduce nomads’ risk of malaria and associated morbidity and mortality, it is important to understand the factors associated to their knowledge of malaria transmission and prevention practices.

Methods
A cross–sectional study among Arab, Dazagada and Fulani pastoral nomadic groups was conducted in February and October 2021. A validated structured electronic questionnaire was administered to assess knowledge of malaria. Attitudes and malaria prevention practices were assessed on the basis of perception of the causes of malaria and the use of a long-lasting insecticide-treated net (LLIN) the day before the survey. Data were analyzed using Chi—square tests and multivariate logistic regression with covariates adjustment.

Results
A total of 278 nomads aged 20 to 65 years were included in the study. Overall, 90.7% of participants surveyed had a good knowledge of malaria. Fulani respondents were more likely to have a good knowledge of malaria than Arab respondents (Adjusted Odd ratio (AOR): 5.00, 95% CI: 1.04–24.03) and households possessing a LLIN were more likely to have a good knowledge of malaria (AOR: 9.66, 95% CI: 1.24–75.36). Most nomad households surveyed reported sleeping under a mosquito net the night before the survey (87.1%) while 98.9% owned a LLIN. Daza respondents (AOR: 0.23, 95% CI: 0.09–0.56) were less likely to use LLINs than Arab respondents. The middle (AOR: 2.78, 95% CI: 1.17–6.62) and wealthier households (AOR: 6.68, 95% CI: 3.19–14.01) were more likely to use LLINs. Knowledge of malaria was associated with the use of LLIN (AOR: 12.77, 95% CI: 1.58–102.99).

Conclusion
There remains a need to improve nomads’ understanding of Plasmodium falciparum-carrying mosquitoes as the vector for malaria transmission and the quality of information provided. Knowledge of malaria and its prevention strategies in nomadic setting lead to the use of LLINs. Further reductions in malaria morbidity can be achieved by improving nomads’ access to LLINs. This study can inform on the design policies to improve nomadic communities’ knowledge of malaria prevention and promoting LLIN use as requested by the national policy against malaria.