bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glioma stem cells and neural stem cells respond differently to BMP4 signaling
Authors: Han Xin-Xin , Cai Chunhui , Yu Li-Ming , Wang Min , Yang Wenhan , Hu Dai-Yu , Ren Jie , Zhu Lu-Ying , Deng Jia-Jia , Chen Qing-Qing , He Hua , Gao Zhengliang 
Published: November 2022

AbstractMalignant glioma is a highly heterogeneous and invasive primary brain tumor characterized by high recurrence rates, resistance to combined therapy, and dismal prognosis. Glioma stem cells (GSCs) are likely responsible for tumor progression, resistance to therapy, recurrence, and poor prognosis owing to their high self-renewal and tumorigenic potential. As a family member of BMP signaling, bone morphogenetic protein4 (BMP4) has been reported to induce the differentiation of GSCs and neural stem cells (NSCs). However, the molecular mechanisms underlying the BMP4-mediated effects in these two cell types are unclear. In this study, we treated hGSCs and hNSCs with BMP4 and compared the phenotypic and transcriptional changes between these two cell types. Phenotypically, we found that the growth of hGSCs was greatly inhibited by BMP4, but the same treatment only increased the cell size of hNSCs. While the RNA sequencing results showed that BMP4 treatment evoked significantly transcriptional changes in both hGSCs and hNSCs, the profiles of differentially expressed genes were distinct between the two groups. A gene set that specifically targeted the proliferation and differentiation of hGSCs but not hNSCs was enriched and then validated in hGSC culture. Our results suggested that hGSCs and hNSCs responded differently to BMP4 stimulation. Understanding and investigating different responses between hGSCs and hNSCs will benefit finding partner factors working together with BMP4 to further suppress GSCs proliferation and stemness without disturbing NSCs.
Effect of stimulant medication on loss of control eating in youth with attention deficit/hyperactivity disorder: a prospective, observational case series study protocol
Authors: Keshen Aaron R. , Hilbert Anja , Taylor Victoria , Harris Anastasia L. , Trappenberg Nami , Sadek Joseph , Frank Guido K.W. , Murray Stuart B. 
Published: November 2022

Abstract
Background
Loss of control eating (LOC-E) in youth predicts the later development of full-syndrome binge-eating disorder (BED), and therefore, could be a relevant target for prevention treatments. To develop these treatments, it is important to understand the underlying disease processes and mechanisms. Based on the putative role of neurocognitive impairments in the pathogenesis of LOC-E, treatments that modulate these neurocognitive factors warrant further exploration. For instance, stimulants are an effective treatment for impulsivity in youth with attention deficit/hyperactivity disorder (ADHD) and have been shown to improve symptoms of BED in adults. Notably, stimulants have not been examined as a treatment for LOC-E in youth. To explore this gap, we aim to measure change in LOC-E episodes and secondary outcomes in youth with comorbid ADHD and LOC-E who are being started on stimulants.

Methods
We will collect prospective observational data on forty 8-to-13-year-old youth diagnosed with comorbid ADHD and LOC-E who are initiating a stimulant for ADHD. Prior to stimulant initiation, participants will complete baseline measures including LOC-E episode frequency in the last 3 months (primary outcome), and secondary outcomes including disordered eating cognitions, emotions and behaviors, ADHD symptom severity, parental LOC-E, impulsivity and reward sensitivity, and anxiety/mood severity. Outcome measurements will be gathered again at 3-months after initiating the stimulant. Within-patient standardized effect sizes with 95% confidence intervals will be calculated from baseline to 3-month follow-up for all outcomes.

Discussion
Many individuals with LOC-E or binge eating do not fully remit over the course of psychotherapy. Whereas psychotherapy may address psychological and sociocultural domains associated with LOC-E, some individuals with neurocognitive impairments (e.g., ADHD) and neurobiological deficits (e.g., low intrasynaptic dopamine or norepinephrine) may benefit from adjunctive treatment that targets those factors. This will be the first study to provide pilot data for future studies that could examine both the effect of stimulants on LOC-E in youth and underlying mechanisms.

Trial registration
Trial registration number: NCT05592119
Can working memory account for EMDR efficacy in PTSD?
Authors: Wadji Dany Laure , Martin-Soelch C. , Camos V. 
Published: November 2022

Abstract
Background:
Although eye movement desensitization and reprocessing (EMDR) has been shown to be effective in the treatment of PTSD for years, it remains controversial due to the lack of understanding of its mechanisms of action. We examined whether the working memory (WM) hypothesis –the competition for limited WM resources induced by the dual task attenuates the vividness and emotionality of the traumatic memory – would provide an explanation for the beneficial effect induced by bilateral stimulation.

Methods:
We followed the Prisma guidelines and identified 11 articles categorized in two types of designs: studies involving participants with current PTSD symptoms and participants without PTSD diagnosis.

Results:
Regardless of the types of studies, the results showed a reduction of vividness and emotionality in the recall of traumatic stimuli under a dual-task condition compared to a control condition, such as recall alone. However, two studies used a follow-up test to show that this effect does not seem to last long.

Conclusion:
Our results provide evidence for the WM hypothesis and suggest that recalling a traumatic memory while performing a secondary task would shift the individual’s attention away from the retrieval process and result in a reduction in vividness and emotionality, also associated with the reduction of symptoms.
Characterization of bioactive substances involved in the induction of bone augmentation using demineralized bone sheets
Authors: Saito Haruka , Chiba-Ohkuma Risako , Yamakoshi Yasuo , Karakida Takeo , Yamamoto Ryuji , Shirai Mai , Ohkubo Chikahiro 
Published: November 2022

Abstract
Purpose
To investigate the bone augmentation ability of demineralized bone sheets mixed with allogeneic bone with protein fractions containing bioactive substances and the interaction between coexisting bioactive substances and proteins.

Methods
Four types of demineralized bone sheets mixed with allogeneic bone in the presence or absence of bone proteins were created. Transplantation experiments using each demineralized bone sheet were performed in rats, and their ability to induce bone augmentation was analysed by microcomputed tomography images. Bioactive substances in bone proteins were isolated by heparin affinity chromatography and detected by the measurement of alkaline phosphatase activity in human periodontal ligament cells and dual luciferase assays. Noncollagenous proteins (NCPs) coexisting with the bioactive substances were identified by mass spectrometry, and their interaction with bioactive substances was investigated by in vitro binding experiments.

Results
Demineralized bone sheets containing bone proteins possessed the ability to induce bone augmentation. Bone proteins were isolated into five fractions by heparin affinity chromatography, and transforming growth factor-beta (TGF-β) was detected in the third fraction (Hep-c). Dentin matrix protein 1 (DMP1), matrix extracellular phosphoglycoprotein (MEPE), and biglycan (BGN) also coexisted in Hep-c, and the binding of these proteins to TGF-β increased TGF-β activity by approximately 14.7% to 32.7%.

Conclusions
Demineralized bone sheets are capable of inducing bone augmentation, and this ability is mainly due to TGF-β in the bone protein mixed with the sheets. The activity of TGF-β is maintained when binding to bone NCPs such as DMP1, MEPE, and BGN in the sheets.
Milk beverages can reduce nutrient inadequacy among Brazilian pre-school children: a dietary modelling study
Authors: Lenighan Yvonne M. , Tassy Marie , Nogueira-de-Almeida Carlos A. , Offord Elizabeth A. , Mak Tsz Ning 
Published: November 2022

AbstractBackgroundPrevious studies found high prevalence of inadequate intakes of vitamins E, D and K, calcium and potassium among Brazilian pre-school children, with suboptimal consumption of dairy products. Dietary modelling was applied to determine the theoretical impact of improving dairy products consumption on nutrient adequacy in 4–5-year-old Brazilian children.MethodsAdherence to the dairy recommendation of two servings/day was calculated using data from the Brazil Kids Nutrition and Health Study (KNHS) (n = 228). Two modelling scenarios were applied to test the impact on nutrient intakes of (1) adding one or two servings of a frequently consumed cow’s milk or a widely available fortified alternative: pre-school children milk (PCM), and of (2) substituting the current milk consumed by PCM. Mean nutrient intakes and percentage of children adhering to the nutrient recommendations were determined at baseline and after applying modelling scenarios.ResultsSeventy-six percent (n = 174) of children did not meet the recommended daily two servings of dairy products, 56% had less than one serving of dairy products on the day of recall. The mean consumption of whole milk (fortified and unfortified) was 147 g/d, yoghurt 114 g/d and cheese 34 g/d. The addition of one serving of cow’s milk demonstrated a 17% reduction in calcium inadequacy, 18% reduction in vitamin A and 3% reduction in zinc inadequacy. Adding one serving of PCM further reduced calcium inadequacy from 87 to 41%, vitamin E from 81 to 37%, and zinc inadequacy by 10%. Replacing the child’s current milk with a PCM resulted in further reduction of micronutrient inadequacies, including calcium, vitamin D and vitamin E.ConclusionsDairy products consumption in pre-school children should be encouraged to reduce nutrient inadequacies. In particular, consumption of PCM would help to reduce calcium, vitamin D and vitamin E inadequacy, nutrients of concern in this population.
Longitudinal study of a procedure for training low-proficiency english language students
Authors: Aljohani Nouf Jazaa 
Published: November 2022

AbstractIn this research study, unlike previous studies that have examined the advantages of peer review for low proficiency students, uses a longitudinal action mixed methods design is used to explore the impact of peer review on the academic writing of students with low English proficiency. The format was a longitudinal action study, which analyzed the written peer review feedback, essays, and reflections of 40 learners. Results showed that at the beginning of the study, the students experienced challenges in identifying problems with rhetorical content of their peers and by the end of 11 weeks, they were able to provide succinct peer feedback that included examples and explanations of classmates’ grammatical and rhetorical errors. Results also showed improvement in their own writing skills. Implications for implementing peer review in foriegn language writing contexts are discussed.
COVID-19 pandemic and the changing views of mobility: the case of Nepal–Malaysia migration corridor
Authors: Wahab Andika , Hamidi Mashitah 
Published: November 2022

AbstractFor decades, Malaysia has been heavily dependent on unskilled and temporarily contracted migrant workers to fulfil labour gaps in the country. While Malaysia’s economy continues to rely on migrant workers, the COVID-19 pandemic has further aggravated their precarious working and living conditions. In-depth interviews with Nepali migrant workers and community leaders in Malaysia and Nepal in 2021 revealed the incidence of labour rights violations, compounded by the lack of access to justice and effective remedies. Besides, workers are allegedly no longer benefiting from the competitive wages, subsequently limiting the value of their remittance to Nepal. We argue that these incidents serve as the drivers of the changing views of mobility, eventually influencing the emigration environment in which the social construction of migration exists in Nepal. This study examines the migratory realities in the Nepal–Malaysia migration corridor during the pandemic, subsequently contributing to current debate on the aspiration–ability model as a class of research.
Brace and Physiotherapeutic Scoliosis Specific Exercises (PSSE) for Adolescent Idiopathic Scoliosis (AIS) treatment: a prospective study following Scoliosis Research Society (SRS) criteria
Authors: Karavidas Nikos , Tzatzaliaris Dionysios 
Published: November 2022

Abstract
Background
A growing scientific evidence for conservative treatment of AIS has recently proved that bracing is superior to natural history. Our aim was to investigate the effectiveness of a combined treatment with brace and PSSE for AIS.

Methods
Prospective study, following SRS research inclusion criteria (> 10 years, 25ο – 40ο, Risser 0–2, < 1-year post-menarche, no prior treatment). 102 consecutive patients (87 females-15 males, mean age 12.8 years, Risser 0.48, Cobb Thoracic 29.2ο, Lumbar 27.8ο) received treatment with Cheneau brace and PSSE. Average follow-up time was 26.4 months. A scale from A to C was used to evaluate compliance with brace and PSSE (A: full-compliant, B: partially compliant, C: non-compliant). 7 subjects dropped-out (6.8%), so finally 95 patients included for statistical analysis, using paired t-test.

Results
Sixty-two patients (65.3%) remained stable, 22 improved > 5ο (23.2%) and 11 progressed (11.5%). In-brace correction (IBC) was 49.7% for thoracic and 61.7% for lumbar curves. Analysis of progressed cases revealed that IBC (31.7% for thoracic and 34.4% for lumbar curves) and compliance (81.8% C for brace, 63.6% C for PSSE) was lower than average. Group A for treatment compliance (65.3%), showed significantly better results (70.9% stable, 29.1% improved, 0% progressed).

Conclusion
A combination of bracing and PSSE can effectively treat AIS, according to SRS inclusion criteria. 88.5% of patients avoided progression > 5ο and only 6.4% overpassed 40ο. IBC and compliance are the most important prognostic factors for successful treatment result. Early detection of AIS is also necessary for increased possibilities of effective conservative treatment.
A 72-channel receive array coil allows whole-heart cine MRI in two breath holds
Authors: Klarenberg Hugo , Gosselink Mark , Coolen Bram F. , Leiner Tim , Nederveen Aart J. , Bakermans Adrianus J. , Lamb Hildo J. , Boekholdt S. Matthijs , Froeling Martijn , Strijkers Gustav J. 
Published: November 2022

Abstract
Background
A new 72-channel receive array coil and sensitivity encoding, compressed (C-SENSE) and noncompressed (SENSE), were investigated to decrease the number of breath-holds (BHs) for cardiac magnetic resonance (CMR).

Methods
Three-T CMRs were performed using the 72-channel coil with SENSE-2/4/6 and C-SENSE-2/4/6 accelerated short-axis cine two-dimensional balanced steady-state free precession sequences. A 16-channel coil with SENSE-2 served as reference. Ten healthy subjects were included. BH-time was kept under 15 s. Data were compared in terms of image quality, biventricular function, number of BHs, and scan times.

Results
BHs decreased from 7 with C-SENSE-2 (scan time 70 s, 2 slices/BH) to 3 with C-SENSE-4 (scan time 42 s, 4–5 slices/BH) and 2 with C-SENSE-6 (scan time 28 s, 7 slices/BH). Compared to reference, image sharpness was similar for SENSE-2/4/6, slightly inferior for C-SENSE-2/4/6. Blood-to-myocardium contrast was unaffected. C-SENSE-4/6 was given lower qualitative median scores, but images were considered diagnostically adequate to excellent, with C-SENSE-6 suboptimal. Biventricular end-diastolic (EDV), end-systolic (ESV) and stroke volumes, ejection fractions (EF), cardiac outputs, and left ventricle (LV)-mass were similar for SENSE-2/4/6 with no systematic bias and clinically appropriate limits of agreements. C-SENSE slightly underestimated LV-EDV (-6.38 ± 6.0 mL, p < 0.047), LV-ESV (-7.94 ± 6.0 mL, p < 0.030) and overestimated LV-EF (3.16 ± 3.10%; p < 0.047) with C-SENSE-4. Bland-Altman analyses revealed minor systematic biases in these variables with C-SENSE-2/4/6 and for LV-mass with C-SENSE-6.

Conclusions
Using the 72-channel coil, short-axis CMR for quantifying biventricular function was feasible in two BHs where SENSE slightly outperformed C-SENSE.
Funding has no effect on clinical outcomes of total joint arthroplasty emerging technologies: a systematic review of bibliometrics and conflicts of interest
Authors: Via Garrhett G. , Brueggeman David A. , Lyons Joseph G. , Ely Isabelle C. , Froehle Andrew W. , Krishnamurthy Anil B. 
Published: November 2022

Abstract
Background
The use of new total joint arthroplasty technologies, including patient-specific implants/instrumentation (PSI), computer-assisted (CA), and robotic-assisted (RA) techniques, is increasing. There is an ongoing debate regarding the value provided and potential concerns about conflicts of interest (COI).

Methods
PRISMA guidelines were followed. PubMed, MEDLINE, and Web of Science databases were searched for total hip and knee arthroplasties, unicompartmental knee arthroplasties (UKA), PSI, CA, and RA. Bibliometric data, financial COI, clinical/functional scores, and patient-reported outcomes were assessed.

Results
Eighty-seven studies were evaluated, with 35 (40.2%) including at least one author reporting COI, and 13 (14.9%) disclosing industry funding. COI and industry funding had no significant effects on outcomes (P = 0.682, P = 0.447), and there were no significant effects of conflicts or funding on level of evidence (P = 0.508, P = 0.826). Studies in which author(s) disclosed COI had significantly higher relative citation ratio (RCR) and impact factor (IF) than those without (P < 0.001, P = 0.032). Subanalysis demonstrated RA and PSI studies were more likely to report COI or industry funding (P = 0.045). RA (OR = 6.31, 95% CI: 1.61–24.68) and UKA (OR = 9.14, 95% CI: 1.43–58.53) had higher odds of reporting favorable outcomes than PSI.

Conclusions
Author COIs (about 40%) may be lower than previously reported in orthopedic technologies/techniques reviews. Studies utilizing RA and PSI were more likely to report COI, while RA and UKA studies were more likely to report favorable outcomes than PSI. No statistically significant association between the presence of COIs and/or industry funding and the frequency of favorable outcomes or study level of evidence was found.

Level of evidence
Level V Systematic Review
Diagnosis of subclinical psoriatic arthritis in patients with psoriasis using CASPAR criteria: a sonographic study
Authors: El-Sherif Sherine Mahmoud , Gaweesh Tamer , Genedy Rasha Mahmoud 
Published: November 2022

Abstract
Background
We aimed at screening for subclinical psoriatic arthritis (PsA) among psoriatic patients without musculoskeletal complaints using ultrasonography of the lower limbs and finding the best predictor for its development.

Results
Subclinical inflammatory articular affection was found by ultrasound in 33 patients, among whom 26 had psoriatic nail affection. According to CASPAR criteria, those 26 patients could be diagnosed as PsA (subclinical). The only statistically significant difference between psoriatic patients with PsA and those without was the mean quadriceps tendon thickness as well as the presence of enthesophytes and bilateral quadriceps thickening. The best and only predictor for subclinical PsA was the presence of enthesophytes.

Conclusion
Ultrasound was more sensitive than clinical examination in detecting subclinical psoriatic arthritis which is highly prevalent in patients with psoriasis even in the absence of manifest arthritic complaints. The best and only predictor for subclinical PsA was the presence of enthesophytes.
PSAT089 Lack of Effect of Metformin in Murine Pheochromocytoma Model
Authors: Ghayee Hans K , Vanova Katerina Hadrava , Pacak Karel , Uher Ondrej 
Published: November 2022

Abstract
Metformin, a lipophilic biguanide inhibiting hepatic gluconeogenesis and improving peripheral utilization of glucose, is a well-established medication for the management of type 2 diabetes. Furthermore, metformin has been shown to have pleiotropic effects targeting oxidative phosphorylation via complex I inhibition. Thus, it regulates the energy supply to cells from mitochondrial complex I respiration along with reduced glycolytic metabolism. Several pre-clinical in vitro studies on cell lines derived from pheochromocytoma (PHEO) have suggested anti-proliferative potential of metformin which hints that metformin could be used as a potent candidate for PHEO anti-cancer therapy. However, these results have yet to be confirmed in vivo.
Murine PHEO MPC cells and human progenitor hPheo1 cells were treated with metformin in vitro to study the effect on cell proliferation and ATP production. Mice were injected with MTT cells subcutaneously and when the tumors reached the average volume of 120.2±48.1 mm3, they were randomized into 3 groups (n=5) treated with vehicle, 125 mg/kg metformin i.p. daily (6 times a week), or the drug dissolved into drinking water (5 mg/ml). Tumor progression and survival data were collected.
Metformin effectively decreased PHEO cell proliferation and overall ATP production in dose-dependent manner in vitro. Tumor progression was similar in both metformin-treated groups and control group with no significant effect on the growth or mice survival. Even though the metformin showed antiproliferative effects in both murine and human derived cells in vitro, we did not observe tumor growth limitation in our model of murine pheochromocytoma in vivo. However, further studies including newer models and variable treatment conditions including timing, are needed to clarify the lack of efficacy in vivo.
Presentation: Saturday, June 11, 2022 1:00 p.m. - 3:00 p.m.