bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fluorogenic probe for predicting treatment response in non-small cell lung cancer with EGFR-activating mutations
Authors: Deng Hui , Lei Qian , Wang Chengdi , Wang Zhoufeng , Chen Hai , Wang Gang , Yang Na , Huang Dan , Yu Quanwei , Yao Mengling , Xiao Xue , Zhu Guonian , Cheng Cheng , Li Yangqian , Li Feng , Tian Panwen , Li Weimin 
Published: November 2022

AbstractTherapeutic responses of non-small cell lung cancer (NSCLC) to epidermal growth factor receptor (EGFR) - tyrosine kinase inhibitors (TKIs) are known to be associated with EGFR mutations. However, a proportion of NSCLCs carrying EGFR mutations still progress on EGFR-TKI underlining the imperfect correlation. Structure-function-based approaches have recently been reported to perform better in retrospectively predicting patient outcomes following EGFR-TKI treatment than exon-based method. Here, we develop a multicolor fluorescence-activated cell sorting (FACS) with an EGFR-TKI-based fluorogenic probe (HX103) to profile active-EGFR in tumors. HX103-based FACS shows an overall agreement with gene mutations of 82.6%, sensitivity of 81.8% and specificity of 83.3% for discriminating EGFR-activating mutations from wild-type in surgical specimens from NSCLC patients. We then translate HX103 to the clinical studies for prediction of EGFR-TKI sensitivity. When integrating computed tomography imaging with HX103-based FACS, we find a high correlation between EGFR-TKI therapy response and probe labeling. These studies demonstrate HX103-based FACS provides a high predictive performance for response to EGFR-TKI, suggesting the potential utility of an EGFR-TKI-based probe in precision medicine trials to stratify NSCLC patients for EGFR-TKI treatment.
Aqueous synthesis of highly functional, hydrophobic, and chemically recyclable cellulose nanomaterials through oxime ligation
Authors: Subbotina Elena , Ram Farsa , Dvinskikh Sergey V. , Berglund Lars A. , Olsén Peter 
Published: November 2022

AbstractCellulose nanofibril (CNF) materials are candidates for the sustainable development of high mechanical performance nanomaterials. Due to inherent hydrophilicity and limited functionality range, most applications require chemical modification of CNF. However, targeted transformations directly on CNF are cumbersome due to the propensity of CNF to aggregate in non-aqueous solvents at high concentrations, complicating the choice of suitable reagents and requiring tedious separations of the final product. This work addresses this challenge by developing a general, entirely water-based, and experimentally simple methodology for functionalizing CNF, providing aliphatic, allylic, propargylic, azobenzylic, and substituted benzylic functional groups. The first step is NaIO4 oxidation to dialdehyde-CNF in the wet cake state, followed by oxime ligation with O-substituted hydroxylamines. The increased hydrolytic stability of oximes removes the need for reductive stabilization as often required for the analogous imines where aldehyde groups react with amines in water. Overall, the process provides a tailored degree of nanofibril functionalization (2–4.5 mmol/g) with the possible reversible detachment of the functionality under mildly acidic conditions, resulting in the reformation of dialdehyde CNF. The modified CNF materials were assessed for potential applications in green electronics and triboelectric nanogenerators.
Priming self-assembly pathways by stacking block copolymers
Authors: Russell Sebastian T. , Bae Suwon , Subramanian Ashwanth , Tiwale Nikhil , Doerk Gregory , Nam Chang-Yong , Fukuto Masafumi , Yager Kevin G. 
Published: November 2022

AbstractBlock copolymers spontaneously self-assemble into well-defined nanoscale morphologies. Yet equilibrium assembly gives rise to a limited set of structures. Non-equilibrium strategies can, in principle, expand diversity by exploiting self-assembly’s responsive nature. In this vein, we developed a pathway priming strategy combining control of thin film initial configurations and ordering history. We sequentially coat distinct materials to form prescribed initial states, and use thermal annealing to evolve these manifestly non-equilibrium states through the assembly landscape, traversing normally inaccessible transient structures. We explore the enormous associated hyperspace, spanning processing (annealing temperature and time), material (composition and molecular weight), and layering (thickness and order) dimensions. We demonstrate a library of exotic non-native morphologies, including vertically-oriented perforated lamellae, aqueduct structures (vertical lamellar walls with substrate-pinned perforations), parapets (crenellated lamellae), and networks of crisscrossing lamellae. This enhanced structural control can be used to modify functional properties, including accessing regimes that surpass their equilibrium analogs.
Dual roles and evolutionary implications of P26/poxin in antagonizing intracellular cGAS-STING and extracellular melanization immunity
Authors: Yin Mengyi , Kuang Wenhua , Wang Qianran , Wang Xi , Yuan Chuanfei , Lin Zhe , Zhang Huanyu , Deng Fei , Jiang Haobo , Gong Peng , Zou Zhen , Hu Zhihong , Wang Manli 
Published: November 2022

AbstractP26, a homolog of the viral-encoded nuclease poxin that neutralizes the cGAS-STING innate immunity, is widely distributed in various invertebrate viruses, lepidopteran insects, and parasitoid wasps. P26/poxin from certain insect viruses also retains protease activity, though its biological role remains unknown. Given that many P26s contain a signal peptide, it is surmised that P26 may possess certain extracellular functions. Here, we report that a secretory baculoviral P26 suppresses melanization, a prominent insect innate immunity against pathogen invasion. P26 targets the cofactor of a prophenoloxidase-activating protease, and its inhibitory function is independent of nuclease activity. The analysis of P26/poxin homologs from different origins suggests that the ability to inhibit the extracellular melanization pathway is limited to P26s with a signal peptide and not shared by the homologs without it. These findings highlight the independent evolution of a single viral suppressor to perform dual roles in modulating immunity during virus-host adaptation.
The gas-phase formation mechanism of iodic acid as an atmospheric aerosol source
Authors: Finkenzeller Henning , Iyer Siddharth , He Xu-Cheng , Simon Mario , Koenig Theodore K. , Lee Christopher F. , Valiev Rashid , Hofbauer Victoria , Amorim Antonio , Baalbaki Rima , Baccarini Andrea , Beck Lisa , Bell David M. , Caudillo Lucía , Chen Dexian , Chiu Randall , Chu Biwu , Dada Lubna , Duplissy Jonathan , Heinritzi Martin , Kemppainen Deniz , Kim Changhyuk , Krechmer Jordan , Kürten Andreas , Kvashnin Alexandr , Lamkaddam Houssni , Lee Chuan Ping , Lehtipalo Katrianne , Li Zijun , Makhmutov Vladimir , Manninen Hanna E. , Marie Guillaume , Marten Ruby , Mauldin Roy L. , Mentler Bernhard , Müller Tatjana , Petäjä Tuukka , Philippov Maxim , Ranjithkumar Ananth , Rörup Birte , Shen Jiali , Stolzenburg Dominik , Tauber Christian , Tham Yee Jun , Tomé António , Vazquez-Pufleau Miguel , Wagner Andrea C. , Wang Dongyu S. , Wang Mingyi , Wang Yonghong , Weber Stefan K. , Nie Wei , Wu Yusheng , Xiao Mao , Ye Qing , Zauner-Wieczorek Marcel , Hansel Armin , Baltensperger Urs , Brioude Jérome , Curtius Joachim , Donahue Neil M. , Haddad Imad El , Flagan Richard C. , Kulmala Markku , Kirkby Jasper , Sipilä Mikko , Worsnop Douglas R. , Kurten Theo , Rissanen Matti , Volkamer Rainer 
Published: November 2022

AbstractIodine is a reactive trace element in atmospheric chemistry that destroys ozone and nucleates particles. Iodine emissions have tripled since 1950 and are projected to keep increasing with rising O3 surface concentrations. Although iodic acid (HIO3) is widespread and forms particles more efficiently than sulfuric acid, its gas-phase formation mechanism remains unresolved. Here, in CLOUD atmospheric simulation chamber experiments that generate iodine radicals at atmospherically relevant rates, we show that iodooxy hypoiodite, IOIO, is efficiently converted into HIO3 via reactions (R1) IOIO + O3 → IOIO4 and (R2) IOIO4 + H2O → HIO3 + HOI + (1)O2. The laboratory-derived reaction rate coefficients are corroborated by theory and shown to explain field observations of daytime HIO3 in the remote lower free troposphere. The mechanism provides a missing link between iodine sources and particle formation. Because particulate iodate is readily reduced, recycling iodine back into the gas phase, our results suggest a catalytic role of iodine in aerosol formation.
Scalable algorithm simplification using quantum AND logic
Authors: Chu Ji , He Xiaoyu , Zhou Yuxuan , Yuan Jiahao , Zhang Libo , Guo Qihao , Hai Yongju , Han Zhikun , Hu Chang-Kang , Huang Wenhui , Jia Hao , Jiao Dawei , Li Sai , Liu Yang , Ni Zhongchu , Nie Lifu , Pan Xianchuang , Qiu Jiawei , Wei Weiwei , Nuerbolati Wuerkaixi , Yang Zusheng , Zhang Jiajian , Zhang Zhida , Zou Wanjing , Chen Yuanzhen , Deng Xiaowei , Deng Xiuhao , Hu Ling , Li Jian , Liu Song , Lu Yao , Niu Jingjing , Tan Dian , Xu Yuan , Yan Tongxing , Zhong Youpeng , Yan Fei , Sun Xiaoming , Yu Dapeng 
Published: November 2022

AbstractImplementing quantum algorithms on realistic devices requires translating high-level global operations into sequences of hardware-native logic gates, a process known as quantum compiling. Physical limitations, such as constraints in connectivity and gate alphabets, often result in unacceptable implementation costs. To enable successful near-term applications, it is crucial to optimize compilation by exploiting the capabilities of existing hardware. Here we implement a resource-efficient construction for a quantum version of AND logic that can reduce the compilation overhead, enabling the execution of key quantum circuits. On a high-scalability superconducting quantum processor, we demonstrate low-depth synthesis of high-fidelity generalized Toffoli gates with up to 8 qubits and Grover’s search algorithm in a search space of up to 64 entries. Our experimental demonstration illustrates a scalable and widely applicable approach to implementing quantum algorithms, bringing more meaningful quantum applications on noisy devices within reach.
Significance of elevated serum and hepatic NOD-like receptor pyrin domain containing 3 (NLRP3) in hepatitis C virus-related liver disease
Authors: Aggan Hoda El , Mahmoud Sabah , Deeb Nevine El , Eleishi Islam , El-Shendidi Assem 
Published: November 2022

AbstractNOD-like receptor pyrin domain containing 3 (NLRP3) is a microbial and danger signal sensor that acts as a regulator of inflammation via activation of Caspase-1 (CASP1) and has been identified as a major contributor to human liver diseases. The present study was conducted to investigate the association between NLRP3 and the progression of hepatitis C virus (HCV)-related liver disease. Serum NLRP3 levels were analyzed in 49 patients with chronic HCV infection and 18 healthy controls and liver tissues from 34 patients were examined to assess the protein expression of NLRP3 and its activation marker CASP1 using immunohistochemical staining. The results showed that the median serum NLRP3 levels was significantly higher in HCV-infected patients compared with healthy controls (1040 pg/ml vs 695 pg/ml respectively, P < 0.001) and were positively correlated with hepatic NLRP3 and CASP1 expression (r = 0.749, P < 0.001 and r = 0.557, P = 0.001 respectively). The NLRP3 levels in serum and the liver significantly increased with worsening liver pathology and showed positive correlations with serum aminotransferases levels, HCV viremia, and albumin-bilirubin score (P < 0.05). The receiver operating characteristic curve analysis revealed a high diagnostic performance of serum NLRP3 in determining the extent of liver necroinflammation, fibrosis, and steatosis (area under the curve = 0.951, 0.971, and 0.917 respectively, P < 0.001). In conclusion, NLRP3 plays an important role in liver disease progression during HCV infection via CASP1 activation and might be a promising therapeutic target. Serum NLRP3 could be an additional biomarker for liver inflammation and fibrosis.
Scrutinized the inherent spin half-metallicity and thermoelectric response of f-electron-based RbMO3 (M = Np, Pu) perovskites: a computational assessment
Authors: Sofi Mudasir Younis , Gupta Dinesh C. 
Published: November 2022

AbstractIn the hunt for novel materials, we present self-consistent ab initio simulations of the structural stability, electronic profile, and transport properties of f-electron-based RbMO3 (M = Np, Pu) perovskites within the context of density functional theory. The structural stability and thermodynamic concerns are fixed by relaxing the crystal structure and computing the energy of formation, respectively. Furthermore, the decisive physical features of given materials have been outlined using the optimised lattice constant retrieved from structural optimizations. The ground state magnetic phase stability is ascertained by minimizing Birch Murnaghan's equation of state in distinct magnetic phases, upholding the ferromagnetic phase (FM) as the ground state magnetic phase, which is further backed by positive Curie Wiess constant values. To specify the electronic structure, a mix of the two approximations GGA and GGA + mBJ has been executed, both of which assert the half-metallic character, culminating in 100% spin polarisation at the Fermi level. The study of the magnetic moment and Curie temperature of each material has further been assessed in the present study. Apart from half-metallicity, the thermoelectric response of the present materials is quantified by exploring the chemical potential dependency of several transport parameters like Seebeck coefficient, electrical and thermal conductivity, power factor, etc. Moreover, the thermoelectric competence has been tested using a zT calculation, adapting values of 1.01 and 0.987 at 300 K for RbNpO3 and RbPuO3, respectively. The high electronic zT at encompassing temperatures uncovers the significant utility of these materials in both low-and high-temperature thermoelectric device applications. In essence, the comprehensive survey of these alloys could certainly open up their possibilities in spintronics, thermoelectric, and solid-state (RTG) device applications.
Vasectomy reversal outcomes in men after testosterone therapy
Authors: Bash Jasper C. , Lo Jamie O. , Kapadia Akash A. , Mason Malachi , Hedges Jason C. 
Published: November 2022

AbstractThe prevalence of males on testosterone therapy (TT) seeking vasectomy reversal (VR) is rising. As medical therapy (MT) to recover spermatogenesis after TT has been previously described, our study’s objective is to present our institution’s management and outcomes of VR in men previously on TT. We performed a retrospective case series of vasectomy patients on TT with subsequent VR by a single microsurgeon between March, 2010 and March, 2022. 14 men undergoing VR during the study period met inclusion criteria. The median age at VR was 43 years with a median obstructive interval of 11 years. Median time from MT to VR was 5 months. Post-operative semen analysis was performed in 10 men and all demonstrated patency. 2 patients had very low sperm counts secondary to continuing TT following VR contrary to medical advice and 5 men with patency achieved pregnancy. Our study noted a high rate of vasovasostomy (VV) (96%) and sustained patency despite a 12-year median obstructive interval. Our findings support favorable outcomes with less stringent VV indications after MT in patients previously on TT that desire VR. The use of MT reduces the recommended wait times for VR after TT discontinuation by more than half.
Recognizing intertwined patterns using a network of spiking pattern recognition platforms
Authors: Amiri Masoud , Jafari Amir Homayoun , Makkiabadi Bahador , Nazari Soheila 
Published: November 2022

AbstractArtificial intelligence computing adapted from biology is a suitable platform for the development of intelligent machines by imitating the functional mechanisms of the nervous system in creating high-level activities such as learning, decision making and cognition in today's systems. Here, the concentration is on improvement the cognitive potential of artificial intelligence network with a bio-inspired structure. In this regard, four spiking pattern recognition platforms for recognizing digits and letters of EMNIST, patterns of YALE, and ORL datasets are proposed. All networks are developed based on a similar structure in the input image coding, model of neurons (pyramidal neurons and interneurons) and synapses (excitatory AMPA and inhibitory GABA currents), and learning procedure. Networks 1–4 are trained on Digits, Letters, faces of YALE and ORL, respectively, with the proposed un-supervised, spatial–temporal, and sparse spike-based learning mechanism based on the biological observation of the brain learning. When the networks have reached the highest recognition accuracy in the relevant patterns, the main goal of the article, which is to achieve high-performance pattern recognition system with higher cognitive ability, is followed. The pattern recognition network that is able to detect the combination of multiple patterns which called intertwined patterns has not been discussed yet. Therefore, by integrating four trained spiking pattern recognition platforms in one system configuration, we are able to recognize intertwined patterns. These results are presented for the first time and could be the pioneer of a new generation of pattern recognition networks with a significant ability in smart machines.
Physical prehabilitation improves the postoperative outcome of associating liver partition and portal vein ligation for staged hepatectomy in experimental model
Authors: Daradics Noemi , Levay Klara , Horvath Ildiko , Kovacs Noemi , Mathe Domokos , Szigeti Krisztian , Szijarto Attila , Fulop Andras 
Published: November 2022

AbstractAiming to improve the postoperative outcome of associating liver partition and portal vein ligation for staged hepatectomy (ALPPS), the effect of physical prehabilitation (PP) was investigated in experimental model. Male Wistar rats (n = 106) divided to PP and sedentary (S) groups underwent ALPPS. Changes in liver weight, Ki67 index and liver volume by magnetic resonance imaging (MRI) were evaluated. Liver function was assessed by laboratory parameters and 99mTc-mebrofenin single-photon emission computed tomography (SPECT) hepatobiliary scintigraphy (HBS). Utilizing endotoxemia model mortality and septic parameters were investigated. Liver mass (p < 0.001), Ki67 index (p < 0.001) and MRI liver volume (p < 0.05) increased in the PP group compared to the S group. Both standard laboratory parameters (p < 0.001) and HBS (p < 0.05) showed enhanced liver function in the PP group compared to the S group. The vulnerability of animals improved in the PP group, as mortality decreased (p < 0.001), while septic laboratory parameters improved (p < 0.05) compared to the S group in the endotoxemia model. Our study demonstrated for the first time the beneficial role of PP on not only volumetric but also functional liver regeneration and postoperative vulnerability after ALLPS.
The optimal concentration of silver nanoparticles in sterilizing fish skin grafts
Authors: Elshahawy Abdelnaby M. , Mahmoud Ghada Abd-Elmonsef , Mokhtar Doaa M. , Ibrahim Ahmed 
Published: November 2022

AbstractCollagen integrity should be considered on using a sterilizing agent for fish skin grafts. This study defined the optimal concentration of silver nanoparticles (Ag NPs) for sterilization of fish skin grafts without disrupting collagen content based on microbiological and histological evaluation. Strips of tilapia skin (n = 5) were randomly allocated to be immersed in Ag NPs solution at different concentrations of 25, 50, 100, and 250 µg/mL, respectively, for 5 min. The treated skin strips underwent bacteriological and histological evaluation. Yeast and fungi were more sensitive to Ag NPs than bacteria. On increasing the nanoparticles concentration, the total counts of aerobic bacteria decrease giving 933.3 ± 28.67, 601 ± 27.66, 288 ± 16.8, 15 ± 4.08 (CFU/cm2 ± S.D) at 25, 50, 100, and 250 µg/mL, respectively, comparing with untreated sample (1453.3 ± 57.92). Yeasts and filamentous fungi also exhibited a similar response, achieving a complete inhibition at 100 and 250 µg/mL. Bacillus cereus and Escherichia coli were the dominant aerobic bacteria, Candida albicans and Rhodotorula glutinis were the dominant aerobic yeasts, whereas Aspergillus niger, Aspergillus fumigatus, and Rhizopus stolonifer were the dominant aerobic fungi. The collagen fibers were loose with a wavey pattern at 25 µg/mL, wavey and slightly disorganized at 50 µg/mL, highly disorganized at 100 µg/mL, and compactly arranged and slightly loose at 250 µg/mL. Ag NPs at a concentration of 250 µg/mL could be considered a reliable and feasible method for the sterilization of fish skin grafts before application on human skin with an effective antimicrobial effect and less disrupting impact on collagen content.
Clinical impact of short limited lumbar fusion for adult spinal deformity with postural and radiological abnormalities
Authors: Nakajima Hideaki , Matsuo Hideaki , Naruse Hiroaki , Watanabe Shuji , Honjoh Kazuya , Shoji Kazuki , Kubota Arisa , Matsumine Akihiko 
Published: November 2022

AbstractExtensive surgical spinopelvic fusion for patients with adult spinal deformity (ASD) to achieve optimal radiological parameters should be avoided. The aim of this study was to review clinical and imaging findings in patients with ASD with postural and radiological abnormalities who underwent a novel three-level limited lumbar fusion as two-stage surgery in an attempt to propose a better tolerated alternative to spinopelvic long fusion to the pelvis. The subjects were 26 patients with a minimum follow-up period of 2 years. Cobb angle, C7 sagittal vertical axis, and pelvic incidence (PI) minus lumbar lordosis (LL) were significantly improved after surgery and maintained at follow-up. Most radiological parameters were corrected with lateral interbody fusion (LIF) as the initial surgery, and few with posterior fusion. PI-LL remained high after limited lumbar fusion, but scores on patient-based questionnaires and sagittal and coronal tilt in gait analysis improved. In cases with postoperative progression of proximal junctional kyphosis (11.5%), residual L1–L2 local kyphosis after LIF was the most significant radiological feature. In some cases of ASD with postural abnormalities, short limited lumbar fusion gives sufficient postoperative clinical improvement with preservation of spinal mobility and activities of daily living. The range of fixation should be determined based on radiological parameters after LIF to avoid postoperative complications.