bluuebunny/crossref_metadata_2025_split huggingface dataset:
CLINICAL MANIFESTATIONS AND DIAGNOSIS OF CO-INFECTION OF COVID-19, TUBERCULOSIS AND OPPORTUNISTIC PULMONARY INFECTIONS IN LATE-STAGE HIV PATIENTS
Authors: Mishina A.V. , Mishin V.Yu. , Shashenkov I. V. 
Published: April 2022

AbstractObjectiveThe purpose of the study was to investigate the specific features of clinical manifestations and diagnosis of co-infection of COVID-19, tuberculosis and opportunistic pulmonary infections in late-stage HIV patients.Design27 patients with co-infection of COVID-19, tuberculosis, opportunistic pulmonary infections and late-stage HIV infection with immunodeficiency without antiretroviral therapy (group 1) and 27 patients with equivalent parameters but without COVID-19 (group 2) were examined.ResultsThe patients of the group 1 and group 2 are the persons with social maladjustment and substance addiction. All of them have concomitant viral hepatitis B/C, COPD, opportunistic pulmonary infections and similar clinical and radiological manifestations, which can only be differentiated with microbiological and molecular genetic studies. The patients with co-infection of COVID-19, tuberculosis and HIV pose a high risk of transmission of infection to healthy persons in view of non-adherence to examination and treatment.ConclusionTo prevent the spread of infection among the healthy population, it is necessary to arrange in a mandatory manner an active and regular COVID-19 testing of all patients with tuberculosis/HIV co-infection, especially of late-stage HIV patients without antiretroviral therapy, in the tuberculosis care unit for HIV-infected persons at the tuberculosis dispensary.SettingThere are few data on the specific features of clinical manifestations of co-infection of COVID-19, tuberculosis (TB) and opportunistic pulmonary infections (OPI) in late-stage HIV patients with immunodeficiency.ObjectiveStudy purpose is to investigate the specific features of clinical manifestations and diagnosis of co-infection of COVID-19, TB and OPI in late-stage HIV patients with immunodeficiency.DesignFifty-four (54) patients admitted for inpatient treatment at the TB Clinical Hospital N 3 named after Zakharyin were enrolled in this prospective study. The participants were assigned to two groups: group 1 (main) and group 2 (comparison group). Group 1 consisted of twenty-seven (27) aged 28-52 patients (18 men (66.7 ± 9.1%) and 9 women (33.3 ± 9.1%)) with known co-infection of COVID-19, sputum smear-positive for M. tuberculosis (MBT) pulmonary tuberculosis (PTB) and with late-stage HIV infection in progression phase without antiretroviral therapy (ART). Group 2 included 27 patients who were selected using the “copy-pair” method and were completely identical to the patients of the main group (with virtually the same age, sex, social parameters and clinical and laboratory indicators), but without diagnosis of COVID-19.
The impact of COVID-19 pandemic on bronchiolitis (lower respiratory tract infection) due to respiratory syncytial virus: A systematic review and meta-analysis
Authors: Sabeena Sasidharanpillai , Ravishankar Nagaraja , Robin Sudandiradas , Pillai Sabitha Sasidharan 
Published: April 2022

AbstractBackgroundThe COVID-19 pandemic has changed the epidemiology of RSV infection which accounts for most bronchiolitis cases and viral pneumonias in infants.AimThis systematic review and meta-analysis aimed to quantitatively assess the effect of COVID-19 pandemic on respiratory syncytial virus (RSV) associated bronchiolitis among hospitalised infants globally.MethodsThe study protocol was registered in the PROSPERO database (CRD42022314000) and was designed based on PRISMA guidelines updated in May 2020. An electronic search of PubMed/MEDLINE, Scopus and Google Scholar was carried out for articles regarding the impact of the COVID-19 pandemic on bronchiolitis or lower respiratory tract infection due to the respiratory syncytial virus in English published between January 2019 and March 2022. The meta-analysis component was modified appropriately to synthesise the pooled proportion of infants having RSV-associated bronchiolitis before the COVID-19 pandemic in 2019 and during the pandemic with 95% confidence interval (CI).ResultsWe screened 189 articles and systematically reviewed fifty studies reporting RSV-associated bronchiolitis cases in infants before the pandemic in 2019 and during the pandemic in 2020/2021. Eight qualified studies from Europe and China, which reported RSV-bronchiolitis both in 2019 and in 2020/21 were pooled by random-effects meta-analysis. These studies comprised 109,186 symptomatic cases of bronchiolitis before the pandemic in 2019 and 61,982 cases in 2020-2021. The quantitative analysis included laboratory-confirmed RSV infection in 7691 infants with bronchiolitis reported before the pandemic in 2019. Meanwhile, during the pandemic, 4964 bronchiolitis cases were associated with RSV infection. The pooled proportion of RSV-associated bronchiolitis cases before the pandemic in 2019 was 16.74% (95% CI 11.73, 22.43%, 95% prediction interval 0.032, 34.16). The pooled proportion of confirmed RSV cases during the pandemic in 2020/2021 was 19.20 % (95% CI 12.01, 27.59%, 95% prediction interval 0.046, 42.35).ConclusionThere was an increase in RSV activity after the relaxation of stringent public health measures during the COVID-19 pandemic.Key Messages (Provide appropriate messages of about 35-50 words to be printed in centre box)This systematic review and meta-analysis reports the pooled proportion of RSV associated bronchiolitis cases in 2019 (before the COVID-19 pandemic) and during the pandemic.Eight observational studies from China and Europe were qualified for the meta-analysis.A decline in reported cases of bronchiolitis was observed during the COVID-19 pandemic which might be attributed to non-pharmaceutical measures and a fall in the hospitalisation rates of respiratory non-SARS-CoV-2 infections.The pooled proportion of RSV positivity rate among bronchiolitis cases was more during the COVID-19 pandemic.
Neurofeedback training for improving motor performance in healthy adults: A systematic review and meta-analysis
Authors: Onagawa Ryoji , Muraoka Yoshihito , Hagura Nobuhiro , Takemi Mitsuaki 
Published: April 2022

AbstractNeurofeedback training (NFT) refers to a training where the participants voluntarily aim to manipulate their own brain activity using the sensory feedback abstracted from their brain activity. NFT has attracted attention in the field of motor learning for its potential to become an alternative or additional training method for general physical training. In this study, a systematic review of NFT studies for motor performance improvements in healthy adults and a meta-analysis on the effectiveness of NFT were conducted. To identify relevant studies published between January 1st, 1990 to August 3rd, 2021, a computerized search was performed using the databases, Web of Science, Scopus, PubMed, JDreamIII, and Ichushi-Web. Thirty-two studies were identified for the qualitative synthesis and 13 randomized controlled trials (286 subjects) for the meta-analysis. The meta-analysis revealed significant effects of NFT for motor performance improvement examined at the timing after the last NFT session (standardized mean difference = 0.96, 95% CI = 0.40–1.53), but with the existence of publication biases and substantial heterogeneity among the trials. Subsequent subgroup meta-analysis demonstrated reliable benefits when the NFT is performed longer than 1 week. The effectiveness of NFT for each motor performance measurement (e.g., speed, accuracy, and hand dexterity) remains unclear because of high heterogeneity or due to small sample size. Further accumulation of empirical NFT studies for motor performance improvement will be necessary to provide reliable evidence about the NFT effects on specific motor skills and to safely incorporate NFT into real-world scenarios.
Ultrasensitivity of Microtubule severing rate on the concentration of free Tubulin
Authors: Shiff Chloe E. , Kondev Jane , Mohapatra Lishibanya 
Published: April 2022

AbstractCytoskeletal structures aid in cell polarization, motility, and intracellular transport. Their functions are predicated on the rapid turnover of cytoskeleton proteins, which is achieved by the coordinated effort of multiple regulatory proteins whose dynamics are not well understood. In-vitro experiments have shown that free tubulin can repair nanoscale damages of microtubules created by severing proteins. Based on this observation, we propose a model for microtubule severing as a competition between the processes of damage spreading and tubulin-induced repair. Using theory and simulations, we demonstrate that this model is in quantitative agreement with in vitro experiments. We predict the existence of a critical tubulin concentration above which severing becomes rare but fast, and hypersensitive to the concentration of free tubulin. Further we show that this hypersensitivity leads to a dramatic increase in the dynamic range of steady-state microtubule lengths, when lengths are controlled by severing. Our work demonstrates how synergy between tubulin and severing proteins can lead to novel dynamical properties of microtubules.
Aquaporin 4 is differentially increased and depolarized in association with tau and amyloid-beta
Authors: Kecheliev Vasil , Boss Leo , Maheshwari Upasana , Konietzko Uwe , Keller Annika , Razansky Daniel , Nitsch Roger M. , Klohs Jan , Ni Ruiqing 
Published: April 2022

AbstractNeurovascular-glymphatic dysfunction plays an important role in Alzheimer’s disease and has been analyzed mainly in association with amyloid-beta (Aβ) pathology. The neurovascular-glymphatic link with tauopathies has not been well elucidated. Here, we aimed to investigate the alterations in the neurovasculature and map the aquaporin 4 (AQP4) distribution and depolarization associated with tau and Aβ. Perfusion, susceptibility weighted imaging and structural magnetic resonance imaging (MRI) were performed in the pR5 P301L mouse model of 4-repeat tau and the arcAβ mouse model of amyloidosis. Immunofluorescence staining was performed using antibodies against AQP4, CD31, astroglia (GFAP, s100β), phospho-tau (AT-8) and Aβ (6E10) in brain tissue slices from P301L, arcAβ and nontransgenic mice. P301L mice showed regional atrophy, preserved cerebral blood flow and reduced cerebral vessel density compared to nontransgenic mice, while arcAβ mice showed cerebral microbleeds and reduced cerebral vessel density. AQP4 depolarization and peri-tau enrichment in the hippocampus and increased AQP4 levels in the forebrain and hippocampus were detected in P301L mice compared to nontransgenic mice. In comparison, cortical AQP4 depolarization and cortical/hippocampal peri-plaque increases were observed in arcAβ mice. Increased s100β-GFAP fluorescence intensities indicative of reactive astrocytes were detected surrounding tau inclusions in P301L mice and Aβ plaques in arcAβ mice. In conclusion, we observed a divergent region-specific AQP4 increase and association with phospho-tau and Aβ pathologies.
Light diffusion in layered media: A numerical study in the spatial and time-domains
Authors: Helton Michael , Zerafa Samantha , Vishwanath Karthik , Mycek Mary-Ann 
Published: April 2022

ABSTRACTAccurate and efficient forward models of photon migration in heterogeneous geometries are important for many applications of light in medicine because many biological tissues exhibit a layered structure, with each layer having independent optical properties and thickness. Unfortunately, closed form analytical solutions are not readily available for layered tissue-models, and often are modeled using computationally expensive numerical techniques or theoretical approximations that limit accuracy and real-time analysis. Here, we develop an open-source accurate, efficient, and stable numerical routine to solve the diffusion equation in the steady-state and time-domain for a layered cylinder tissue model with an arbitrary number of layers and specified thickness and optical coefficients. We show that the steady-state (< 0.1 ms) and time-domain (< 0.5 ms) fluence (for an 8-layer medium) can be calculated with absolute numerical errors approaching machine precision. The numerical implementation increased computation speed by 3 to 4 orders of magnitude compared to previously reported theoretical solutions in layered media. We verify our solutions asymptotically to homogeneous tissue geometries using closed form analytical solutions to assess convergence and numerical accuracy. Approximate solutions to compute the reflected intensity are presented which can decrease the computation time by an additional 2-3 orders of magnitude. We also compare our solutions for 2, 3, and 5 layered media to gold-standard Monte Carlo simulations in layered tissue models of high interest in biomedical optics (e.g. skin/fat/muscle and brain). The presented routine could enable more robust real-time data analysis tools in heterogeneous tissues that are important in many clinical applications such as functional brain imaging and diffuse optical spectroscopy.
Diet induced obesity and type 2 diabetes drives exacerbated sex-associated disease profiles in K18-hACE2-mice challenged with SARS-CoV-2
Authors: Lee Katherine S. , Russ Brynnan P. , Wong Ting Y. , Horspool Alexander M. , Winters Michael T. , Barbier Mariette , Bevere Justin R. , Martinez Ivan , Damron F. Heath , Cyphert Holly A. 
Published: April 2022

AbstractSARS-CoV-2 infection results in wide-ranging disease manifestation from asymptomatic to potentially lethal. Infection poses an increased threat of severity to at-risk populations including those with hypertension, diabetes, and obesity. Type 2 Diabetes (T2DM), is characterized, in part, by insulin insensitivity and impaired glucose regulation. T2DM patients have increased disease severity and poorer outcomes with COVID-19. We utilized the diet-induced obesity (DIO) model of Type 2 Diabetes in SARS-CoV-2-susceptible K18-hACE2 transgenic mice to better understand the obesity co-morbidity. Female DIO, but not male DIO mice challenged with SARS-CoV-2 were observed to have shortened time to morbidity compared to normal diet mice. Increase in susceptibility to SARS-CoV2 in female DIO was associated with increased total viral RNA burden compared to male mice. RNAseq analysis was performed on the lungs of non-challenged, challenged, females, males, of either normal diet or DIO cohorts to determine the disease specific transcriptional profiles. DIO female mice had more total activated genes than normal diet mice after challenge; however, male mice experienced a decrease. GO term analysis revealed the DIO condition increased interferon response signatures and interferon gamma production following challenge. Male challenged mice had robust expression of antibody-related genes suggesting antibody producing cell localization in the lung. DIO reduced antibody gene expression in challenged males. Collectively this study establishes a preclinical T2DM/obesity co-morbidity model of COVID-19 in mice where we observed sex and diet specific responses that begin to explain the effects of obesity and diabetes on COVID-19 disease.
CORTICOTROPIN-RELEASING FACTOR- DOPAMINE INTERACTIONS IN MALE AND FEMALE MACAQUE: BEYOND THE CLASSIC VTA
Authors: Kelly E.A. , Love T.M. , Fudge J.L. 
Published: April 2022

ABSTRACTDopamine (DA) is involved in stress and stress-related illnesses, including many psychiatric disorders. Corticotropin-releasing factor (CRF) plays a role in stress responses and targets the ventral midbrain DA system which is composed of DA and non-DA cells, and divided into specific subregions. Although CRF inputs to the midline A10 nuclei ('classic VTA') are known, in monkeys, CRF-containing terminals are also highly enriched in the expanded A10 parabrachial pigmented nucleus (PBP) and in the A8 retrorubral field subregions. We characterized CRF-labeled synaptic terminals on DA (tyrosine hydroxylase, TH+) and non-DA (TH-) cell types in the PBP and A8 regions using immuno-reactive electron microscopy (EM) in male and female macaques. CRF labeling was present mostly in axon terminals, which mainly contacted TH-negative dendrites in both subregions. Most CRF-positive terminals had inhibitory (symmetric) profiles. In both PBP and A8, CRF symmetric (inhibitory) synapses onto TH-negative dendrites were significantly greater than asymmetric (excitatory) profiles. This overall pattern was similar in males and females, despite shifts in the size of these effects between regions depending on sex. Because stress and gonadal hormone shifts can influence CRF expression, we also did hormonal assays over a 6-month time period, and found little variability in basal cortisol across similarly housed animals at the same age. Together our findings suggest that at baseline, CRF-positive synaptic terminals in the primate PBP and A8 are poised to regulate DA indirectly through synaptic contacts onto non-DA neurons.
The Toll and Imd pathway, the complement system and lectins during immune response of the nemertean Lineus ruber
Authors: Orús-Alcalde Andrea , Børve Aina , Hejnol Andreas 
Published: April 2022

AbstractInnate immunity is the first line of defense against pathogens. In animals, the Toll pathway, the Imd pathway, the complement system, and lectins are well-known mechanisms involved in innate immunity. Although these pathways and systems are well understood in vertebrates and arthropods, they are understudied in other invertebrates. In order to shed light on immunity in the nemertean Lineus ruber, we performed a transcriptomic survey and identified the main components of the Toll pathway (e.g. myD88, dorsal/dif/NFκB-p65), the Imd pathway (e.g. imd, relish/NFκB-p105/100), the complement system (e.g. C3, cfb) and some lectins (FreD-Cs and C-lectins). In situ hybridization showed that TLRβ1, TLRβ2 and imd and are expressed in the nervous system, the complement gene C3-1 is expressed in the gut and the lectins in the nervous system, the blood, and the gut. To reveal their potential role in defense mechanisms, we performed immune challenge experiments, in which Lineus ruber specimens were exposed to the gram-negative bacteria Vibrio diazotrophicus. Our results show the upregulation of specific components of the Toll pathway (TLRα3, TLRβ1, and TLRβ2), the complement system (C3-1), and lectins (c-lectin2 and fred-c5). Therefore, similarly to what occurs in other invertebrates, our study shows that components of the Toll pathway, the complement system and lectins are involved in the immune response in the nemertean Lineus ruber. The presence of these pathways and systems in Lineus ruber, but also in other spiralians, in protostomes and in deuterostomes suggest that these pathways and systems were involved in the immune response in the stem species of Bilateria.
Cost-efficient boundary-free surface patterning achieves high effective-throughput of time-lapse microscopy experiments
Authors: Liang Guohao , Yin Hong , Ding Fangyuan 
Published: April 2022

AbstractTime-lapse microscopy plays critical roles in the studies of cellular dynamics. However, to set up a time-lapse movie experiments is not only laborious but also with low output, mainly due to the cell-losing problem (i.e., cell moving out of limited field of view), especially in a long time recording. To overcome these issues, we have designed a cost-efficient way that enables cell patterning on the imaging surfaces without any physical boundaries. Using mouse embryonic stem cells as an example system, we have demonstrated that our boundary-free patterned surface solves the cell-losing problem without disturbing their cellular phenotype. Statistically, the presented system increases the effective-throughput of time-lapse microscopy experiments by order of magnitude.
A systematic review of the experiences of minority language users in health and social care research
Authors: Spencer Dr Llinos Haf , Cooledge Mrs Beryl Ann , Hoare Dr Zoe 
Published: April 2022

AbstractTo obtain the most rigorous results in health and social care research (HSCR), it is important to have a representative sample. Culture can sometimes be overlooked but can be important to the individual concerned. Ethnic minorities are often not accounted for in HSCR. Migration, equality, and diversity issues are now important priorities and therefore need to be considered by researchers. Therefore, it is important to explore the literature examining the experiences of minority language users in HSCR. A systematic review (SR) of the literature was conducted to answer the research question. SPIDER framework and Cochrane principles were utilized for the SR. Five databases were searched, yielding 5311 papers initially. A SR protocol was developed and published in PROSPERO. Available from: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020225114analysis. Following the title and abstract review by two authors, 74 papers were included, and a narrative account was provided. Six themes were identified: 1. Disparities in health care; 2. Maternal health; 3. Mental health; 4. Methodology in health research; 5. Migrant and minority health care; 6. Racial and ethnic gaps in health care. It was evident that language barriers (including language proficiency) and cultural barriers still exist in terms of recruitment, possibly effecting the validity of the results. Several papers acknowledged language barriers but did not act on this information. Ethnic minorities’ needs are not completely addressed in HSCR. Despite research acknowledging different cultures over the past 40 years, there is a need for this to be fully acknowledged and embedded in the research process. We propose that future research should include details of ethnicity and languages spoken so that readers can understand the sample composition to be able to interpret the results in the best way, recognising the significance of culture and language.2-4 Key messages, detailing the main points made in the paperEthnic minorities’ needs are not completely addressed in health and social care research. Despite research acknowledging different cultures over the past 40 years, there is a need for this to be fully acknowledged and embedded in the research process.Ethnic background and languages spoken by the research participants should be identified and addressed throughout the research process (from design of the study to dissemination of findings).Language and cultural preferences should be appropriately considered/included in the analysis.Future research should include details of ethnicity and languages spoken so that readers can understand the sample composition to be able to interpret the results in the best way, recognising the significance of culture and language.