bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA RP11-551L14.4 suppresses breast cancer development by inhibiting the expression of miR-4472
Authors: Wang Bin , Chen Hang , Yang Rui , Xing Lei , Chen Chuan , Chen Junxia 
Published: December 2022

Background
Previous studies have been reported that long non-coding RNA (lncRNA) can regulate the expression of genes which are involved in many important cellular processes The potential role of lncRNA RP11-551L14.4 in the development of breast cancer and the possible regulatory mechanisms was investigated.

Methods
Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to analyze RP11-551L14.4 expression in 36 paired breast cancer tissues and adjacent tissues. The expression of RP11-551L14.4 in multiple breast cancer cell lines was detected by qRT-PCR. Meanwhile, overexpression of RP11-551L14.4 models was established using lentivirus in BT474 and T47D breast cancer cells. Cell counting kit-8 (CCK-8), cell colony formation and cell cycle assays were performed to detect the effects of RP11-551L14.4 on the biological function of breast cancer cells. Besides, bioinformatics techniques, dual luciferase reporter gene assay and rescue experiments were used to investigate the potential mechanisms.

Results
RP11-551L14.4 expression was negatively associated with the advanced tumor stage. Breast cancer patients with low RP11-551L14.4 expression manifested a poorer prognosis. The results of qRT-PCR showed that RP11-551L14.4 expression in breast cancer tissues was significantly lower than in adjacent tissues. Meanwhile, overexpression of RP11-551L14.4 significantly decreased the cell proliferation and cell cycle. Bioinformatics technology showed that RP11-551L14.4 could complementarily bind to miR-4472. qRT-PCR results indicated that the expression levels of miR-4472 and RP11-551L14.4 in breast cancer were negatively correlated. Luciferase reporter gene assay showed that miR-4472 remarkably decreased the relative luciferase activity of the wild-type RP11-551L14.4 vector. miR-4472 is a direct target gene of RP11-551L14.4. miR-4472 levels were reduced, and repulsive guidance molecule A (RGMA) mRNA or protein levels were increased after overexpression of RP11-551L14.4 in the breast cancer cells. miR-4472 reversed the effects caused by RP11-551L14.4 in breast cancer cells.

Conclusion
RP11-551L14.4 expression was remarkably decreased in breast cancer tissues and cells. RP11-551L14.4 may inhibit the malignant progression of breast cancer by regulating miR-4472 expression.
Phytochemical Content and Antibacterial Effect of Ethanolic and Methanolic Extracts of Pleurotus ostreatus Mushroom
Authors: Anyakorah Caroline Iruoma , Agbaje-Daniels Folashade 
Published: December 2022

Aims: Microbial resistance is a threat to the successful treatment of microbial infections. Mushrooms are known to possess antimicrobial and antioxidant potential which could be inhibitory to some pathogenic organisms. The aim was to assess the phytochemicals and antimicrobial potential of Pleurotus ostreatus extracts on some pathogenic organisms.
Methodology: The study was conducted at the Bells University of Technology, Nigeria, between December 2019 and August 2020. A 10% dried powder of Pleurotus ostreatus was extracted in absolute ethanol and methanol and evaporated in a water bath at 50 oC. Extracts were reconstituted in dimethyl Sulfoxide (DMSO) at 12.5, 25, 50, and 100% respectively. Antibiotic effects of extracts were tested on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus saprophyticus by agar well diffusion method and compared with standard antibiotic discs. DMSO and the solvents served as positive and negative controls. Ethanolic extract was subjected to Gas Chromatography-Mass Spectrometer.
Results: The extracts exhibited varying inhibitory effects on the organisms except on E coli at 12.5% ethanolic extract. The inhibition zones increased with extract concentration from 2-12 mm and 4-14 mm in ethanolic and methanolic extracts respectively. The inhibitory effect of standard antibiotics was higher 17-25 mm except for erythromycin (13-14 mm). Test organisms were resistant to Beta Lactams (augmentin, cefuroxime, and ceftazidime) and sensitive to Aminoglycoside (gentamicin), Fluoroquinolones (ofloxacin), Ciprofloxacin, and erythromycin. GC/MS revealed the presence of 30 organic compounds, amongst them were sugar, sugar alcohols, alkaloids, amines, fatty acids, esters, aldehydes, and alcohols. The most abundant were oleic acid (33.75%) and 9-octadecenoic acid (Z)-2-hydroxy-1- (21.21%). Phytochemicals are heterocyclic compounds found in natural products.
Conclusion: P. ostreatus has been revealed to possess phytochemicals of medical relevance with potential for pharmacological application. The implication is that Pleurotus ostreatus extracts could serve as a lead to novel drug discoveries which could enhance health and nutritional well-being.
The Perks of Online Selling: Shared Experiences and Defying Challenges
Authors: Nolasco Dyanika P. , Gumayagay Mary Joy E. , Marquez Lorelie B. , Hernandez Danisse Mae P. , Barayuga Syrille Joyce R. 
Published: December 2022

Aim: This study aimed at exploring and documenting the experiences of online sellers and determine their struggles on online selling amidst the pandemic.
Research Design: This qualitative research utilized phenomenology as strategy of inquiry to better understand the experiences and challenges of online sellers.
Place and Duration of Study: The study was conducted during the pandemic in a state university in the Philippines.
Methodology: The study used purposive sampling involving eight participants who have been doing part-time online selling during the pandemic. These participants got engaged in the sphere of online selling to gain additional profit for themselves and family.
Results: Online sellers were faced with various challenges as they do their sphere. They need to deal with impatient and inconsiderate buyers, unpredictable stocks and prices, competition in the market and unguaranteed quality of items. But despite of these challenges and struggles, online sellers describe online selling not only as a platform for marketing but a tool to gain new friends and widen their market horizons. It has also become a source of fun and income. Online selling also tests sellers to strategize and use their skills and creativity so they can be successful at it.
Conclusions: Online selling can be a decent opportunity and a challenging endeavor that one can venture on. This became even more challenging during the occurrence of pandemic but it also offered additional profit and advantages for those doing it.
DNA Droplets: Intelligent, Dynamic Fluid
Authors: Udono Hirotake , Gong Jing , Sato Yusuke , Takinoue Masahiro 
Published: December 2022

AbstractBreathtaking advances in DNA nanotechnology have established DNA as a promising biomaterial for the fabrication of programmable higher‐order nano/microstructures. In the context of developing artificial cells and tissues, DNA droplets have emerged as a powerful platform for creating intelligent, dynamic cell‐like machinery. DNA droplets are a microscale membrane‐free coacervate of DNA formed through phase separation. This new type of DNA system couples dynamic fluid‐like property with long‐established DNA programmability. This hybrid nature offers an advantageous route to facile and robust control over the structures, functions, and behaviors of DNA droplets. This review begins by describing programmable DNA condensation, commenting on the physical properties and fabrication strategies of DNA hydrogels and droplets. By presenting an overview of the development pathways leading to DNA droplets, it is shown that DNA technology has evolved from static, rigid systems to soft, dynamic systems. Next, the basic characteristics of DNA droplets are described as intelligent, dynamic fluid by showcasing the latest examples highlighting their distinctive features related to sequence‐specific interactions and programmable mechanical properties. Finally, this review discusses the potential and challenges of numerical modeling able to connect a robust link between individual sequences and macroscopic mechanical properties of DNA droplets.
Topographic Manipulation of Graphene Oxide by Polyaniline Nanocone Arrays Enables High‐Performance Solar‐Driven Water Evaporation
Authors: Zhao Xin , Meng Xiangtong , Zou Hongqi , Wang Zhenhao , Du Yadong , Shao Yuan , Qi Jun , Qiu Jieshan 
Published: December 2022

AbstractTuning the surface topography of solar evaporators is of significance for boosting light absorption and enhancing solar‐to‐vapor efficiency. Herein, a novel strategy to manipulate the surface topography of graphene oxide (GO) via electrostatic assembly coupled with in situ polymerizations of aniline is reported. The GO surface is fully hybridized with the polyaniline (PANI) nanocone arrays, manifesting periodic structures with highly foldable configurations. Additionally, the PANI arrays tune the surface chemistry of GO and retard the redispersion of GO into water, thus enabling corresponding composite (PG) robust structural durability. Featuring these intriguing attributes, when applied as an evaporator in pure water, the PG delivers an improved evaporation performance of 1.42 kg m−2h−1and a high evaporation efficiency of 96.6% under one sun illumination. Further investigations reveal that the periodically conical structures of PANI over GO surface strengthen light absorption via multiple reflections and facilitate heat localization. Desalination test substantiates the reliability of PG for practical freshwater production. The numerical simulations and optical microscopy observation exhibit the surface topography‐strengthened vapor generation effect. This study sheds new light on the rational manipulation of surface topography of photothermal materials for high‐efficiency solar evaporation.
Many Facets of Photonic Crystals: From Optics and Sensors to Energy Storage and Photocatalysis
Authors: Lonergan Alex , O'Dwyer Colm 
Published: December 2022

AbstractThe ability to selectively redirect specific wavelengths of light has attracted a lot attention for photonic crystal materials. Presently, there is a wealth of research relating to the fabrication and application of photonic crystal materials. There are a number of structures that fall into the category of a photonic crystal; 1D, 2D, and 3D ordered structures can qualify as a photonic crystal, provided there exists ordered repeating lattices of dielectric material with a sufficient refractive index contrast. The optical responses of these structures, namely the associated photonic bandgap or stopband, are of particular interest for any application involving light. The sensitivity of the photonic bandgap to changes in lattice size or refractive index composition creates the possibility for accurate optical sensors. Optical phenomena involving reduced group velocity at wavelengths on the edge of the photonic bandgap are commonly exploited for photocatalytic applications. The inherent reflectivity of the photonic bandgap has created applications in optical waveguides or as solar cell reflector layers. There are countless examples of research attempting to exploit these facets of photonic crystal behavior for improved material design. Here, the role of photonic crystals is reviewed across a wide variety of disciplines; cataloging the ways in which these structures have enhanced specific applications. Particular emphasis is placed on providing an understanding of the specific function of the tunable optical response in photonic crystals in relation to their application.
Alternating Flow Field Design Improves the Performance of Proton Exchange Membrane Fuel Cells
Authors: Qin Zhengguo , Huo Wenming , Bao Zhiming , Tongsh Chasen , Wang Bowen , Du Qing , Jiao Kui 
Published: December 2022

AbstractThe flow field structure of a proton exchange membrane fuel cell (PEMFC) is a determining factor for improving the cell power density. In this study, a universal alternating flow field design for the first time is proposed, which arranges structural units with different flow resistances in an alternating way to significantly improve the gas transfer rate into the electrode, with the advantages of easy machining and low pumping loss. Based on the design, it is proposed and tested large‐scale fuel cells with three novel flow fields by combining a parallel channel, baffled channel, serpentine channel, and narrowed channel. The results show that the design can significantly enhance the gas supply efficiency and that the novel baffled flow field improves the PEMFC performance by 23% with low pumping loss. The design employed in the study offers additional options for flow field optimization and contributes to the early achievement of next‐generation ultrahigh power density fuel cells.
Unveiling True Limits of Electrochemical Performance of Organic Cathodes in Multivalent Batteries through Cyclable Symmetric Cells
Authors: Lužanin Olivera , Moškon Jože , Pavčnik Tjaša , Dominko Robert , Bitenc Jan 
Published: December 2022

AbstractMultivalent batteries are often hyped as a next‐generation high‐energy density battery technology, but in reality, both literature reports and practical research are plagued by poor reproducibility of electrochemical results. Within the present work, we take a look at the electrochemical testing of organic cathodes that can be used with a variety of mono‐ and multivalent cations and propose a cyclable symmetric cell approach, already applied to the field of lithium‐ion batteries. By using a model organic system based on poly(anthraquinonyl sulfide) (PAQS) active material, we demonstrate that the symmetric cell approach elegantly removes the limitations of multivalent metal anodes, and for the first time, reveals the full potential of organic cathodes in multivalent batteries. Furthermore, symmetric cells enable reliable EIS measurements on organic cathodes and open a pathway to optimize electrochemical/transport parameters through the design of next‐generation organic cathode materials and advanced electrode design(s). We suggest that the cyclable symmetric cell approach should be generally applicable in multivalent and all other batteries where electrochemical characterization requires the elimination of the counter electrode contribution.
Innovation und Vergaberecht – ein Widerspruch?
Authors: Jungmann Stefan 
Published: December 2022

AbstractProjekte, die mit öffentlichen Mitteln gefördert werden, unterliegen regelmäßig dem formalen Vergaberecht, den Vorschriften zu Beschaffungen der öffentlichen Hand. Auf den ersten Blick scheinen das formale Regelwerk und die Innovation – in diesem Fall die Errichtung des Carbonbetonhauses CUBE – im Widerspruch zu stehen. Das Vergaberecht kennt jedoch ausreichend Ausnahmetatbestände, die unter Beachtung der vergaberechtlichen Grundsätze wie Gleichbehandlung, Transparenz oder Verhältnismäßigkeit ein flexibles Vergabeverfahren ermöglichen. Im Bericht wird beschrieben, wie das Verfahren organisiert wurde, welche Zwischenschritte, Schwierigkeiten und Änderungen vorgenommen wurden und wie es letztendlich erfolgreich abgeschlossen werden konnte. Entscheidend für diesen Erfolg waren die enge, transparente und zuführende Zusammenarbeit aller Beteiligten sowie die Bereitschaft, den Prozess wiederholt an den erforderlichen Leistungsumfang, die Baumethodik sowie den bautechnologischen und zeitlichen Ablauf anzupassen.
Der Bauwerkszustand als Herausforderung für die Rückbauplanung – Methoden und Empfehlungen für erforderliche Untersuchungen
Authors: Schacht Gregor , Barr Caroline , Diers Johannes , Fritsch Christina , Marx Steffen 
Published: December 2022

AbstractFür die Tragwerksplanung beim Rückbau existieren aktuell keine verbindlich eingeführten technischen Regelwerke, die den Ingenieuren bei Fragen der Bewertung bestehender und z. T. geschädigter Brückenbauwerke Hilfestellung leisten. Häufig ist es erforderlich, komplexe statische Bauzustände nachzuweisen, die maßgeblich vom Zustand der Brücke abhängig sind. Einzige Informationsquelle bilden in der Regel die Bestandsunterlagen, die aber nur begrenzte Informationen über den tatsächlichen Bauwerkszustand beinhalten. Um das Bestandsrisiko zu minimieren und die Planungs‐ und Ausschreibungssicherheit insbesondere für große und komplexe Bauwerke zu erhöhen, bieten bauwerksdiagnostische Untersuchungen ein großes Potenzial. Da nicht alle Informationen, wie z. B. die Kenntnis über das nachträgliche Verankerungsvermögen durchtrennter Spannglieder, im Vorfeld zu einem Rückbau erfasst bzw. sicher bewertet werden können, sind experimentelle Untersuchungen während des Rückbaus häufig unabdingbar. Hierzu gibt es bislang nur unzreichende technische und vertragliche Regeln. Der Beitrag stellt praktische Erfahrungen und Potenziale solcher Untersuchungen vor.
Antioxidant Effects of Rhodoxanthin from Potamogeton crispus L. on H2O2‐Induced RAW264.7 Macrophages Cells
Authors: Fu Cong , Dong Hongchun , Wang Xiaozhen , Wang Huirui , Zheng Yang , Ren Dandan , He Yunhai , Liu Shu , Wang Qiukuan 
Published: December 2022

AbstractPotamogeton crispus L. (P. crispus) is the type of a widely distributed perennial herbs, which is rich in rhodoxanthin. In this research work, five antioxidant indexes in vitro were selected to study the antioxidant activity of rhodoxanthin from P. crispus (RPC). A model of hydrogen peroxide (H2O2) ‐induced oxidative damage in RAW264.7 cells was established to analyze the antioxidant effect and potential mechanism of RPC. The levels of ROS, MDA and the activities of oxidation related enzymes by H2O2 were determined by enzyme linked immunosorbent assay (ELISA). The mRNA expression of Nrf‐2, HO‐1, SOD1 and SOD2 was measured by qRT‐PCR assay. According to the results, RPC had free radical scavenging ability for 2, 2‐diphenyl‐1‐trinitrohydrazine (DPPH), 2,2’‐azinobis(3‐ethylbenzo‐thiazoline‐6‐sulfonic acid radical ion) (ABTS), hydroxyl radical and superoxide anion. RPC significantly decreased the level of MDA and ROS and LDH activity, while increased GSH level and activities of SOD, GSH−Px and CAT. It was showed that RPC could increase the mRNA expression of Nrf‐2, HO‐1, SOD1 and SOD2 in RAW264.7 cells in a dose‐dependently manner. In summary, RPC treatment could effectively attenuate the H2O2‐induced cell damage rate, and the mechanism is related to the reduction of H2O2 induced oxidative stress and the activation of Nrf‐2 pathway.
Surfactant‐Assisted Purification of Hydrophobic DNA Nanostructures
Authors: Iwabuchi Shoji , Nomura Shin‐ichiro M. , Sato Yusuke 
Published: December 2022

AbstractPurification of functional DNA nanostructures is an essential step in achieving intended functions because misfolded structures and the remaining free DNA strands in a solution can interact and affect their behavior. However, due to hydrophobicity‐mediated aggregation, it is difficult to purify DNA nanostructures modified with hydrophobic molecules by conventional methods. Herein, we report the purification of cholesterol‐modified DNA nanostructures by using a novel surfactant‐assisted gel extraction. The addition of sodium cholate (SC) to the sample solution before structure folding prevented aggregation; this was confirmed by gel electrophoresis. We also found that adding sodium dodecyl sulfate (SDS) to the sample inhibited structural folding. The cholesterol‐modified DNA nanostructures prepared with SC were successfully purified by gel extraction, and their ability to bind to the lipid membrane surfaces was maintained. This method will facilitate the purification of DNA nanostructures modified with hydrophobic molecules and expand their applicability in the construction of artificial cell‐like systems.
Facile de Novo Sequencing of Tetrazine‐Cyclized Peptides through UV‐Induced Ring‐Opening and Cleavage from the Solid Phase
Authors: Borges Ariane , Nguyen Chi , Letendre Madison , Onasenko Iryna , Kandler Rene , Nguyen Ngoc K. , Chen Jue , Allakhverdova Tamara , Atkinson Emily , DiChiara Bella , Wang Caroline , Petler Noa , Patel Henna , Nanavati Dhaval , Das Samir , Nag Arundhati 
Published: December 2022

AbstractWhile most FDA‐approved peptide drugs are cyclic, the robust cyclization chemistry of peptides and the deconvolution of cyclic peptide sequences by using tandem mass spectrometry render cyclic peptide drug discovery difficult. Here we present the successful design of cyclic peptides on solid phase that addresses both of these problems. We demonstrate that this peptide cyclization method using dichloro‐s‐tetrazine on solid phase allows successful cyclization of a panel of random peptide sequences with various charges and hydrophobicities. The cyclic peptides can be linearized and cleaved from the solid phase by simple UV light irradiation, and we demonstrate that accurate sequence information can be obtained for the UV‐cleaved linearized peptides by using tandem mass spectrometry. The tetrazine linker used in the cyclic peptides can further be explored for inverse electron‐demand Diels‐Alder (IEDDA) reactions for screening or bioconjugation applications in the future.
A ruptured balloon shaft during an angioplasty
Authors: Hammami Rania , Ellouze Tarek , Bahloul Amine , Abid Leila 
Published: December 2022

AbstractWe report a case of balloon shaft rupture during percutaneous coronary intervention. Although the entrapped balloon was not yet deflated when the complication occurred, we successfully retrieved it percutaneously using a trapping technique. This case described a cheap and straightforward technique of device retrieval that helped save our patient.
Pneumonia in the presentation of Kawasaki disease: The syndrome or a sequence of two diseases?
Authors: Khoury Lana , Livnat Galit , Hamad Saied Mohamad , Yaacoby‐Bianu Karin 
Published: December 2022

AbstractTwo cases of Kawasaki disease (KD) presented as persistent lung consolidation associated with Group A Streptococcus and Influenza A co‐infection, which resolved following intravenous immunoglobulin. Thus, pediatricians should consider the diagnosis of KD in the presence of pneumonia that is nonresponsive to antibiotic therapy with prolonged fever and inflammatory reactions.
Hemangioma of the hard palate with invasive growth into the maxillary sinus
Authors: Lobekk Ole Kristian , Pedersen Torbjørn Ø. 
Published: December 2022

AbstractA 55 year‐old woman presented with an invasive hemangioma of the hard palate. One year after partial surgical excision there was no sign of recurrency or oroantral fistulation. No malignant cells could be identified.
Subacute brainstem ischemic syndrome in juvenile neurofibromatosis type 2: An underrecognized condition
Authors: Blauen Aglaë , Lenfant Christine , Duprez Thierry , Nassogne Marie‐Cécile 
Published: December 2022

AbstractWe report the case of a teenager with a neurofibromatosis Type 2 (NF2) presenting a locked‐in syndrome due to a brainstem ischemic syndrome. The presence of sudden or rapidly worsening onset of neurological deficits in NF2 patients, should evoke this underknown entity and not only tumors as predisposed by NF2.
Progressive pulmonary tuberculosis associated with bronchial dilatation: A clinical image
Authors: Razafindrasoa Zamelina Angela , Ravahatra Kiady , Razafimpihanina Sonia Marcelle , Martin Fidy Arnauld , Andriarimanga Diamondra Ombanjanahary , Randrianasolo Davis Avizara , Rakotomizao Jocelyn Robert , Tiaray Harison Michel , Rakotoson Joëlson Lovaniaina , Raharimanana Rondro Nirina 
Published: December 2022

AbstractBronchial dilatation is a potentially serious and irreversible clinical and radiological entity. It is an often underdiagnosed condition, particularly in developing countries. This clinical image presents bronchial dilatations of casual discovery associated with radiographic images suggestive of progressive pulmonary tuberculosis. Thoracic imaging remains crucial in the diagnosis of bronchopulmonary diseases.