bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma proteome profiling of healthy subjects undergoing bed rest reveals unloading‐dependent changes linked to muscle atrophy
Authors: Murgia Marta , Brocca Lorenza , Monti Elena , Franchi Martino V. , Zwiebel Maximilian , Steigerwald Sophia , Giacomello Emiliana , Sartori Roberta , Zampieri Sandra , Capovilla Giovanni , Gasparini Mladen , Biolo Gianni , Sandri Marco , Mann Matthias , Narici Marco V. 
Published: December 2022

AbstractBackgroundInactivity and unloading induce skeletal muscle atrophy, loss of strength and detrimental metabolic effects. Bed rest is a model to study the impact of inactivity on the musculoskeletal system. It not only provides information for bed‐ridden patients care, but it is also a ground‐based spaceflight analogue used to mimic the challenges of long space missions for the human body. In both cases, it would be desirable to develop a panel of biomarkers to monitor muscle atrophy in a minimally invasive way at point of care to limit the onset of muscle loss in a personalized fashion.MethodsWe applied mass spectrometry‐based proteomics to measure plasma protein abundance changes in response to 10 days of bed rest in 10 young males. To validate the correlation between muscle atrophy and the significant hits emerging from our study, we analysed in parallel, with the same pipeline, a cohort of cancer patients with or without cachexia and age‐matched controls. Our analysis resulted in the quantification of over 500 proteins.ResultsUnloading affected plasma concentration of proteins of the complement cascade, lipid carriers and proteins derived from tissue leakage. Among the latter, teneurin‐4 increased 1.6‐fold in plasma at bed rest day 10 (BR10) compared with BR0 (6.E9 vs. 4.3E9, P = 0.02) and decreased to 0.6‐fold the initial abundance after 2 days of recovery at normal daily activity (R + 2, 2.7E9, P = 3.3E‐4); the extracellular matrix protein lumican was decreased to 0.7‐fold (1.2E9 vs. 8.5E8, P = 1.5E‐4) at BR10 and remained as low at R + 2. We identified six proteins distinguishing subjects developing unloading‐mediated muscle atrophy (decrease of >4% of quadriceps cross‐sectional area) from those largely maintaining their initial muscle mass. Among them, transthyretin, a thyroid hormone‐binding protein, was significantly less abundant at BR10 in the plasma of subjects with muscle atrophy compared with those with no atrophy (1.6E10 vs. 2.6E10, P = 0.001). Haptoglobin‐related protein was also significantly reduced in the serum of cancer patients with cachexia compared with that of controls.ConclusionsOur findings highlight a combination or proteomic changes that can be explored as potential biomarkers of muscle atrophy occurring under different conditions. The panel of significant proteomic differences distinguishing atrophy‐prone and atrophy‐resistant subjects after 10 days of bed rest need to be tested in a larger cohort to validate their potential to predict inactivity‐triggered muscle loss in humans.
Governance diversity: Its impact on strategic variation and results
Authors: Samara Ihssan , Nandakumar M. K. , O'Regan Nicholas , Almoumani Hanada M. 
Published: December 2022

AbstractThis paper investigates the impact of four drivers of Governance diversity, namely gender, tenure, age, and educational attainment, on strategic direction and variation. It then incorporates corporate financial results as a moderating variable, testing how it impacts the links between board diversity and strategic variation. Strategic variation or change is assessed based on measuring deviation from past strategies. Our sample consists of 5011 firm‐year observations from 930 firms in the United States between 2010 and 2018. The findings indicate that the four drivers of Governance diversity show a positive relationship between the corporate board of directors and strategic variation/change. However, the strength of the effect depends on overall firm results/performance.
Integrative analysis of transcriptome and metabolome reveals probiotic effects on cecal metabolism in broilers
Authors: Wang Yanfei , Liu Xuan , Jia Hao , Zhang Ruonan , Guan Jiawei , Zhang Lihuan 
Published: December 2022

AbstractBACKGROUNDProbiotics play an important role in the host and have attracted widespread attention as an alternative to antibiotics. Arbor Acres broilers were used in the present experiment and fed different doses of compound probiotics at 1, 5, and 10 g kg−1. The effects of compound probiotics on broiler growth performance and cecal transcriptome and metabolome were investigated.RESULTSWe discovered 425 differentially expressed genes (DEGs; upregulated: 256; downregulated: 169) in the cecal transcriptome study. These DEGs were assigned to fat metabolic pathways, such as the peroxisome proliferator‐activated receptor (PPAR) signaling pathway, according to KEGG analysis. Probiotics downregulated LPL and upregulated PPARα expression in the cecum. In metabolome analysis of the cecum of cecum, we screened 86 differential metabolites and performed KEGG enrichment analysis of these metabolites. The KEGG analysis showed that these differentially expressed metabolites were annotated to nucleotide metabolism‐related pathways, such as purine metabolism. In the cecum, probiotics upregulated the content of guanine, AMP, 3′‐AMP, adenylosuccinate, deoxyguanosine, and ADP‐ribose, whereas they downregulated the content of 5‐hydroxyisourate. Comprehensive transcriptome and metabolome analysis revealed that glycolysis, gluconeogenesis, and glycerophospholipid metabolism pathways were jointly enriched in cecum of broilers fed a probiotic‐containing diet.CONCLUSIONThis study provides valuable information for studying the regulation and gene metabolism network of probiotics on cecal metabolism in broilers. © 2022 Society of Chemical Industry.
Photocurable Nitric Oxide‐Releasing Copolyester for the 3D Printing of Bioresorbable Vascular Stents
Authors: de Oliveira Matheus F. , da Silva Laura C. E. , Catori Daniele M. , Lorevice Marcos V. , Galvão Karen E. A. , Millás Ana L. G. , de Oliveira Marcelo G. 
Published: December 2022

AbstractThe design of bioresorbable vascular stents (BVS) capable of releasing nitric oxide (NO) at the implant site may enable BVS to mimic the antiplatelet, antiproliferative, and pro‐endothelial actions of NO, overcoming complications of BVS such as late thrombosis and restenosis. In this study, the fabrication of BVS composed of methacrylated poly(dodecanediol citrate‐co‐dodecanediol S‐nitroso‐mercaptosuccinate) (mP(DC‐co‐DMSNO)), a novel elastomeric, bioabsorbable, and photocurable copolyester, containing covalently bound S‐nitrosothiol groups in the carbon backbone of the polymer, is reported. The mP(DC‐co‐DMSNO) stents are manufactured via photoinduced 3D printing and allow deployment via a self‐expansion process from a balloon catheter. After deployment, hydration of the stents triggers the release of NO, which is maintained during the slow hydrolysis of the polymer. Real‐time NO release measurements show that by varying the copolyester composition and the strut geometry of the mP(DC‐co‐DMSNO) stents, it is possible to modulate their NO release rate in the range of 30–52 pmol min−1 cm−2. Preliminary biological assays in cell culture show that endothelial cells adhere to the surface of the stents and that NO release favors their endothelization. Thus, mP(DC‐co‐DMSNO) may emerge as a new platform for the fabrication of advanced BVS.
Porphyra haitanensis Polysaccharides Attenuates Blood Lipid via Gut‐Liver Axis in Diet‐Induced High‐Fat Mesocricetus auratus through Multiple Integrated Omics
Authors: Zeng Hongliang , Chen Peilin , Wang Zhiyun , Hu Xiaoke , Zhang Yi , Zheng Baodong 
Published: December 2022

ScopeHyperlipidemia is currently a global public health problem severely affecting people's physical and mental health, as well as their quality of life.Methods and resultsThe present study is aimed at revealing the mechanism of Porphyra haitanensis polysaccharide (PHP) in decreasing blood lipids by acting through gut‐liver axis in Mesocricetus auratus fed a high‐fat diet. PHP significantly prevented increases in serum total cholesterol, triglycerides and low‐density lipoprotein cholesterol, and alleviated damage to liver cells induced by a high‐fat diet M. auratus, in a dose‐dependent manner. PHP promotes proliferation of Muribaculaceae and Faecalibaculum, thereby enhancing the production of butyric acid both in the colon and liver, particularly high‐dose PHP (HPHP). Low‐dose PHP (LPHP) promotes the expression of phosphatidylcholine metabolites and fatty acid transport genes, and inhibits the expression of genes involved in fat degradation (Abhd5), adipogenesis (Me1), fatty acid synthesis (Fasn and Pnpla3), and fatty acid chain elongation (Elovl6) in the liver. However, HPHP inhibits the expression of triglyceride metabolites and promotes the expression of fatty acid transporter (CD36), fatty acid oxidation (Acacb), and peroxisome proliferator‐activated receptor gamma (PPARg) genes in the liver.ConclusionPHP regulates lipid metabolism through the gut microbiota, and the gut‐liver axis plays an important role in its hypolipidemic effects.
Telemedicine in sickle cell disease: Patient, parent, and provider perspectives
Authors: Speller‐Brown Barbara , Carhuas Carlos , Stone Andrew , Connolly Megan , Martin Brenda , Zhang Anqing , Pallapolu Manasa , Darbari Deepika S. 
Published: December 2022

AbstractIntroductionPatients with sickle cell disease (SCD) need frequent health maintenance visits and may face barriers accessing care. Telemedicine, during COVID pandemic, has provided a unique model of care to improve access; however, potential barriers and satisfaction with its use in SCD have not been fully evaluated.ObjectiveTo determine caregiver, patient, and healthcare provider (HCP) perspectives and satisfaction with telemedicine in healthcare delivery.MethodsWe surveyed patients with SCD, caregivers, and HCP, who participated in at least one telemedicine visit from March 2020 to June 2021, using the Telemedicine Usability Questionnaire (TUQ). We also accessed and compared the Press Ganey surveys completed by families who completed a telemedicine or in‐person visit. Data were summarized using descriptive statistics. The internal reliability of TUQ was assessed using Cronbach's coefficient alpha. Press Ganey data comparing satisfaction with telemedicine versus in‐person visits were analyzed by Mann–Whiney U test.ResultsFifty‐two patients/caregivers and 10 HCP completed the survey. Patients/caregivers rated satisfaction “excellent” in the five areas (Usefulness, Ease of use, Effectiveness, Reliability and Satisfaction). HCP rated Usefulness, Ease of use, Effectiveness, Satisfaction as “good,” and Reliability as “excellent.” Press Ganey scores for satisfaction with care for telemedicine and in‐person visits were not statistically different (p > .05).DiscussionWe found high satisfaction for caregivers and patients as well as HCP in the delivery of clinical services via telemedicine for SCD. We suggest that telemedicine is a viable option for this population and may help overcome the barriers SCD families often face accessing care.
Self‐compassion and subjective well‐being: A moderated mediation model of online prosocial behavior and gratitude
Authors: Zeng Pan , Nie Jia , Geng Jingyu , Wang Hongxia , Chu Xiaoyuan , Qi Lin , Wang Pengcheng , Lei Li 
Published: December 2022

AbstractPrevious studies have shown that personal factors serve as mediators in the link between self‐compassion and subjective well‐being (SWB). However, these studies have neglected the possibility that online prosocial behavior (OPB) may serve as a mediator in the link between self‐compassion and SWB. Based on the PERMA theory of well‐being and the protective‐protective model, the current study examined whether OPB would mediate the association between self‐compassion and individual SWB, and whether gratitude would moderate the association between self‐compassion and OPB. A valid sample of 1488 college students (Mage = 19.84; SD = 1.61) filled out questionnaires regarding demographics, self‐compassion, OPB, gratitude, and SWB. Results showed that, after controlling for gender, age, daily online time, and social desirability, OPB mediated the association between self‐compassion and individual SWB, and gratitude strengthened the positive association between self‐compassion and OPB. These findings reveal that high self‐compassionate and grateful people can enhance their subjective well‐being through behaving prosocially online.
Dialkyl‐fluoroquinoxaline–based polymeric donor for binary and ternary organic solar cell applications
Authors: Agneeswari Rajalingam , Ahn Yoomi , Tamilavan Vellaiappillai , Shin Insoo , Shin Chnan‐gi , Park Sung Heum , Jin Youngeup 
Published: December 2022

AbstractHere, we studied the potential of 2,3‐didodecyl‐6‐fluoroquinoxaline–based polymer as an electron‐donor in binary and ternary organic solar cell (OSC) applications. A new wide bandgap polymeric donor, namely poly(4,8‐bis(5‐(2‐ethylhexyl)thiophen‐2‐yl)benzo[1,2‐b:4,5‐b′]dithiophene‐alt‐2,3‐didodecyl‐6‐fluoro‐5,8‐di(thiophen‐2‐yl)quinoxaline) (P(BDTT‐TfQT)), is prepared. Polymer P(BDTT‐TfQT) showed intense absorption between 300 and 650 nm with an optical band gap of 1.83 eV. The determined highest occupied and lowest unoccupied molecular orbitals (highest occupied molecular orbital/lowest unoccupied molecular orbital = −5.28 eV/−3.45 eV) for P(BDTT‐TfQT) are found to be suitable to utilize as an electron donor along with widely used fullerene and non‐fullerene acceptors. The OSCs prepared by using P(BDTT‐TfQT):fullerene blend gave a maximum power conversion efficiency (PCE) of 4.64%, and the PCE further increased to 6.77% by using a non‐fullerene acceptor instead of fullerene. Overall, the improved light absorption results in higher photo‐current and PCE for P(BDTT‐TfQT):non‐fullerene blend compared to that of P(BDTT‐TfQT):fullerene blend. In contrast, the inclusion of 10 vol% of P(BDTT‐TfQT) as a third component in a high energy converting polymer: non‐fullerene blend is found to lower the performance from 16.03% to 14.50% for the resulting ternary OSCs.
Hyperthermostable recombinant human heteropolymer ferritin derived from a novel plasmid design
Authors: Srivastava Ayush K. , Scalcione Lucas J. , Arosio Paolo , Bou‐Abdallah Fadi 
Published: December 2022

AbstractMammalian ferritins are predominantly heteropolymeric species consisting of 2 structurally similar, but functionally and genetically distinct subunit types, called H (Heavy) and L (Light). The two subunits co‐assemble in different H and L ratios to form 24‐mer shell‐like protein nanocages where thousands of iron atoms can be mineralized inside a hollow cavity. Here, we use differential scanning calorimetry (DSC) to study ferritin stability and understand how various combinations of H and L subunits confer aspects of protein structure–function relationships. Using a recently engineered plasmid design that enables the synthesis of complex ferritin nanostructures with specific H to L subunit ratios, we show that homopolymer L and heteropolymer L‐rich ferritins have a remarkable hyperthermostability (Tm = 115 ± 1°C) compared to their H‐ferritin homologues (Tm = 93 ± 1°C). Our data reveal a significant linear correlation between protein thermal stability and the number of L subunits present on the ferritin shell. A strong and unexpected iron‐induced protein thermal destabilization effect (ΔTm up to 20°C) is observed. To our knowledge, this is the first report of recombinant human homo‐ and hetero‐polymer ferritins that exhibit surprisingly high dissociation temperatures, the highest among all known ferritin species, including many known hyperthermophilic proteins and enzymes. This extreme thermostability of our L and L‐rich ferritins may have great potential for biotechnological applications.
qNABpredict: Quick, accurate, and taxonomy‐aware sequence‐based prediction of content of nucleic acid binding amino acids
Authors: Wu Zhonghua , Basu Sushmita , Wu Xuantai , Kurgan Lukasz 
Published: December 2022

AbstractProtein sequence‐based predictors of nucleic acid (NA)‐binding include methods that predict NA‐binding proteins and NA‐binding residues. The residue‐level tools produce more details but suffer high computational cost since they must predict every amino acid in the input sequence and rely on multiple sequence alignments. We propose an alternative approach that predicts content (fraction) of the NA‐binding residues, offering more information than the protein‐level prediction and much shorter runtime than the residue‐level tools. Our first‐of‐its‐kind content predictor, qNABpredict, relies on a small, rationally designed and fast‐to‐compute feature set that represents relevant characteristics extracted from the input sequence and a well‐parametrized support vector regression model. We provide two versions of qNABpredict, a taxonomy‐agnostic model that can be used for proteins of unknown taxonomic origin and more accurate taxonomy‐aware models that are tailored to specific taxonomic kingdoms: archaea, bacteria, eukaryota, and viruses. Empirical tests on a low‐similarity test dataset show that qNABpredict is 100 times faster and generates statistically more accurate content predictions when compared to the content extracted from results produced by the residue‐level predictors. We also show that qNABpredict's content predictions can be used to improve results generated by the residue‐level predictors. We release qNABpredict as a convenient webserver and source code at http://biomine.cs.vcu.edu/servers/qNABpredict/. This new tool should be particularly useful to predict details of protein–NA interactions for large protein families and proteomes.
Metabolic functional redundancy of the CYP9A subfamily members leads to P450‐mediated lambda‐cyhalothrin resistance in Cydia pomonella
Authors: Li Pei‐Rong , Shi Yu , Ju Di , Liu Yu‐Xi , Wang Wei , He Ying‐Shi , Zhang Yu‐Yun , Yang Xue‐Qing 
Published: December 2022

AbstractBACKGROUNDThe evolution of insect resistance to pesticides poses a continuing threat to sustainable pest management. While much is known about the molecular mechanisms that confer resistance in model insects and few agricultural pests, far less is known about fruit pests. Field‐evolved resistance to synthetic insecticides such as lambda‐cyhalothrin has been widely documented in Cydia pomonella, a major invasive pest of pome fruit worldwide, and the increased production of cytochrome P450 monooxygenases (P450s) has been linked to resistance in field‐evolved resistant populations. However, the underlying molecular mechanisms of P450‐mediated insecticide resistance remain largely unknown.RESULTSHere we found that functional redundancy and preference of metabolism by P450s genes in the CYP9A subfamily confer resistance to lambda‐cyhalothrin in Cydia pomonella. A total of four CYP9A genes, including CYP9A61, CYP9A120, CYP9A121, and CYP9A122, were identified from Cydia pomonella. Among these, CYP9A120, CYP9A121, and CYP9A122 were predominantly expressed in the midgut of larvae. The expression levels of these P450 genes were significantly induced by a lethal dose that would kill 10% (LD10) of lambda‐cyhalothrin and were overexpressed in a field‐evolved lambda‐cyhalothrin resistant population. Knockdown of CYP9A120 and CYP9A121 by RNA‐mediated interference (RNAi) increased the susceptibility of larvae to lambda‐cyhalothrin. In vitro assays demonstrated that recombinant P450s expressed in Sf9 cells can metabolize lambda‐cyhalothrin, but with functional redundancy and divergence through regioselectivity of metabolism. CYP9A121 preferred to convert lambda‐cyhalothrin to 2′‐hydroxy‐lambda‐cyhalothrin, whereas CYP9A122 only generated 4′‐hydroxy metabolite of lambda‐cyhalothrin. Although possesses a relatively low metabolic capability, CYP9A120 balanced catalytic competence to generate both 2′‐ and 4′‐metabolites.CONCLUSIONCollectively, these results reveal that metabolic functional redundancy of three members of the CYP9A subfamily leads to P450‐mediated lambda‐cyhalothrin resistance in Cydia pomonella, thus representing a potential adaptive evolutionary strategy during its worldwide expansion. © 2022 Society of Chemical Industry.
The ability of BC3 to remove Cs from nuclear wastewater
Authors: Zhou Nan , Qin Yong , Zhang Yang , Wu Feihong , Yu Wantang , Tan Jie , Cheng Jinjuan , Wu Xijun 
Published: December 2022

AbstractDensity functional theory (DFT) was used to conduct a systematic analysis of the adsorption characteristics of Cs on three two‐dimensional materials: graphene, C3N, and BC3. The adsorption energies of Cs were calculated, and the electronic structure of the system was analyzed. The dependence of adsorption energies on temperatures was taken into account, and the first‐principles‐derived adsorption energies were used to calculate the influence of temperature on the adsorption rate. It was discovered that BC3 is more stable than the other two materials, has good adsorption characteristics, and has performance that is not significantly degraded at high temperatures. The adsorption performances of other radionuclides I and Ag (which also have radionuclides) on BC3, were also calculated to compare with that of Cs. The results show that the adsorption capacity of Cs on BC3 is much greater than that of the two lighter elements. Based on this study, BC3 is a good candidate for removing Cs from nuclear wastewater.
Elevated, FcεRI-Dependent MRGPRX2 Expression on Basophils in Chronic Urticaria
Authors: Bartko Ewa A. , Elberling Jesper , Blom Lars H. , Poulsen Lars K. , Jensen Bettina M. 
Published: December 2022

Abstract

Background
Chronic urticaria (CU) is a skin condition driven by mast cells and basophils. The exact responsiveness profile of these cells, especially regarding the anti-IgE treatment, Omalizumab, is not fully investigated. We sought to characterize the surface activation profile of basophils in CU during Omalizumab treatment and their responsiveness to IgE and non-IgE stimulation.

Methods
Whole blood basophils from 11 CU patients and 10 healthy controls were stimulated with either medium, anti-IgE, fMLP, C5a, or Substance P for 30 min and characterized by flow cytometry.

Results
CU patients showed a broad range of basophil count as opposed to healthy subjects. An increased number of unstimulated CD69+ (p = 0.05), but not CD63+ basophils was observed in CU groups in comparison to healthy. The expression of CD203c and CD200R were comparable between all groups, whilst the FcεRI was reduced with the treatment. Both IgE and non-IgE mediated stimulations upregulated CD63, CD203c and CD200R, but not CD69 in all groups, however, no difference between the groups was observed. Among unstimulated basophils, expression of MRGPRX2 was higher in CU patients after Omalizumab treatment than in the healthy group (2.4% vs. 1.5%, p = 0.01). The anti-IgE stimulation increased the number of MRGPRX2-expressing basophils in the CU group before and after omalizumab as compared to the healthy (p = 0.003; p = 0.005). The fMLP and C5a stimulations showed a similar effect to the IgE-mediated stimulation. The MRGPRX2 ligand, Substance P did not activate basophils.

Conclusion
CU basophils show increased expression of MRGPRX2 after IgE and non-IgE stimulation.