bluuebunny/crossref_metadata_2025_split huggingface dataset:
Angiotensin II Type 2 Receptor Potentiates Skeletal Muscle Satellite Cell Differentiation via the GSK3β/β-catenin Pathway
Authors: Lott C Seth , Chandrasekar Bysani , Delafontaine Patrice , Yoshida Tadashi 
Published: October 2022

AbstractPatients with advanced congestive heart failure (CHF) or chronic kidney disease (CKD) often have increased systemic angiotensin II (Ang II) levels and cachexia. We previously demonstrated that Ang II infusion in rodents results in skeletal muscle wasting and reduced muscle regenerative potential via Ang II type 1 receptor (AT1R) signaling, potentially contributing to cachexia in CHF and CKD. Contrary to AT1R signaling, we found that signaling via Ang II type 2 receptor (AT2R) potentiates skeletal muscle satellite cell (SC) differentiation and muscle regenerative potential. However, mechanisms whereby AT2R regulates SC differentiation and cachexia development remain unknown. In this study, we found that GSK3β activity was significantly suppressed during SC differentiation, whereas it was retained in SCs with AT2R knockdown. AT2R knockdown leads to higher GSK3β and decreased β-catenin activities bothin vitroandin vivo. Treatment with GSK3β inhibitor BIO restored β-catenin activity and differentiation capacity of SCs with AT2R knockdown. Conversely, transgenic overexpression of AT2R in SCs inhibited GSK3β, associated with increased β-catenin activity and SC myogenic capacity bothin vitroandin vivo. Interestingly, AT2R expression in undifferentiated SCs was regulated post-transcriptionally. An increase in systemic Ang II blunted AT2R induction during muscle regeneration. However, overexpression of AT2R restored AT2R levels and myogenesisin vivo. Together, these data suggest that the AT2R/GSK3β/β- catenin signaling pathway could serve as a potential therapeutic target to promote muscle regenerative capacity in chronic disease conditions characterized by heightened activation of the renin-angiotensin system, such as CHF and CKD.
THE ASSOCIATION OF CD47 WITH INTEGRIN Mac-1 REGULATES MACROPHAGE RESPONSES BY STABILIZING THE EXTENDED INTEGRIN CONFORMATION
Authors: Podolnikova Nataly P. , Key Shundene , Wang Xu , Ugarova Tatiana P. 
Published: October 2022

ABSTRACTCD47 is a ubiquitously expressed cell surface integrin-associated protein. Recently, we have demonstrated that integrin Mac-1 (αMβ2, CD11b/CD18, CR3), the major adhesion receptor on the surface of myeloid cells, can be coprecipitated with CD47. However, the molecular basis for the CD47-Mac-1 interaction and its functional consequences remain unclear. Here, we demonstrated that CD47 regulates macrophage functions directly interacting with Mac-1. In particular, adhesion, spreading, migration, phagocytosis, and fusion of CD47-deficient macrophages were significantly decreased. The functional link between CD47 and Mac-1 was validated by co-immunoprecipitation analysis using various Mac-1-expressing cells. In HEK293 cells expressing individual αM and β2 integrin subunits, CD47 has been found to bind both subunits. Interestingly, the amount of CD47 recovered with the free β2 subunit was higher than in the complex with the whole integrin. Furthermore, activating Mac-1-expressing HEK293 cells with PMA, Mn2+, and activating antibody increased CD47 in complex with Mac-1, suggesting greater stability of the complex with integrin in the extended conformation. Notably, on the surface of cells lacking CD47, fewer Mac-1 molecules could convert into an extended conformation in response to activation. The binding site in CD47 for Mac-1 was identified in its constituent IgV domain. The complementary binding sites for CD47 in Mac-1 were localized in integrin epidermal growth factor-like domains 3 and 4 of the β2 and calf-1 and calf-2 domains of the α subunits. These results indicate that Mac-1 forms a lateral complex with CD47, which regulates essential macrophage functions by stabilizing the extended integrin conformation.
A subset of viruses thrives following microbial resuscitation during rewetting of a seasonally dry California grassland soil
Authors: Nicolas Alexa M. , Sieradzki Ella T. , Pett-Ridge Jennifer , Banfield Jillian F. , Taga Michiko E. , Firestone Mary K. , Blazewicz Steven J. 
Published: October 2022

AbstractViruses are abundant, ubiquitous members of soil communities that kill microbial cells, but how they respond to perturbation of soil ecosystems is essentially unknown. Here, we investigated lineage-specific virus-host dynamics in grassland soil following “wet-up”, when resident microbes are both resuscitated and lysed after a prolonged dry period. Quantitative isotope tracing, time-resolved metagenomics and viromic analyses indicated that dry soil held a diverse but low biomass reservoir of virions, of which only a subset thrived following wet-up. Viral richness decreased by 50% within 24 h post wet-up, while viral biomass increased four-fold within one week. Counter to recent hypotheses suggesting temperate viruses predominate in soil, our evidence indicates that wet-up is dominated by viruses in lytic cycles. We estimate that viruses drive a measurable and continuous rate of cell lysis, with up to 46% of microbial death driven by viral lysis one week following wet-up. Thus, viruses contribute to turnover of soil microbial biomass and the widely reported CO2efflux following wet-up of seasonally dry soils.
Re-evaluation and Re-analysis of 152 research exomes five years after the initial report reveals clinically relevant changes in 20%
Authors: Bartolomaeus Tobias , Hentschel Julia , Jamra Rami Abou , Popp Bernt 
Published: October 2022

ABSTRACTIterative re-analysis of NGS results is not well investigated for published research cohorts of rare diseases.We revisited a cohort of 152 consanguineous families with developmental disorders (NDD) reported five years ago. We re-evaluated all reported variants according to diagnostic classification guidelines or our candidate gene scoring system (AutoCaSc) and systematically scored the validity of gene-disease associations. Sequencing data was re-processed using an up-to-date pipeline for case-level re-analysis.In 30/152 (20%) families, we identified a clinically relevant change. Thirteen previously reported (likely) pathogenic variants were re-classified as VUS/benign. In three cases, the gene-disease association (TSEN15, NAPB, and FAR1) validity was judged as limited. We identified 12 new disease causing variants. Two previously reported variants were missed by our updated pipeline due to alignment or reference issues.Our results support the need to re-evaluate screening studies, not only the negative cases but including supposedly solved ones. This also applies in a diagnostic setting. We highlight that the complexity of computational re-analysis for old data should be weighed against the decreasing re-testing costs. Since extensive re-analysis per case is beyond the resources of most institutions, we recommend a screening procedure that would quickly identify the majority (83%) of new variants.
Improvement of performance of the RC beams using the longitudinal spiral reinforcements
Authors: Labibzadeh Mojtaba , Bostan Shirin Farhad , Khajehdezfuly Amin 
Published: October 2022

PurposeThis study aims to investigate the effects of using circular spirals as the longitudinal reinforcing bars on the performance of the concrete beams subjected to four-point bending load.Design/methodology/approachThe effects of using circular spirals as the longitudinal reinforcing bars on the performance of the concrete beams subjected to four-point bending load are investigated in this study. Employing circular spirals as the main longitudinal reinforcement is a novel idea presented in this paper. In this regard, a finite element model of the beam with spiral longitudinal reinforcement was developed. After model verification, several configurations of concrete beams reinforced by longitudinal spirals were simulated under the four-point loading condition.FindingsObtained results showed that using the longitudinal spirals in place of the conventional longitudinal reinforcing bars can improve the bearing capacity of the concrete beam, but at the same time, increases its ductility unacceptably. In other words, the spirals reduce the initial stiffness of the beam significantly. To solve the problem, the authors decided to use the longitudinal spirals as the auxiliary bars added to the main conventional longitudinal bars in the beams. New gained results were satisfactory. By adding the longitudinal spirals to the conventional bars, not only the bearing capacity of the beam increases between 24% and 63%, but also the initial stiffness and ductility of the beam raises between 11%–29% and 3%–57%, respectively, in comparison to the corresponding beam reinforced with conventional longitudinal bars.Originality/valueEmploying circular spirals as the main longitudinal reinforcement is a novel idea presented in this paper.
The link and spillovers between clean energy and fossil fuels market: a systematic literature review
Authors: Xia Summer 
Published: October 2022

PurposeThe global transition into clean energy demands a re-examination of the link between fossil fuels and clean energy markets. This paper aims to identify the key research areas about clean energy and fossil fuels and outlines emerging themes for future research.Design/methodology/approachThis paper uses bibliographic mapping to identify the most influential publications among 500 articles published between 1991 and 2022 (as of March 2022). The first step is the identification of literature for inclusion and data cleaning. Next is producing a citation map using Bibliometrix for analysis and synthesis.FindingsThe research on clean energy and fossil fuels has developed into three areas by examining their dynamic causality, return/risk spillovers, and volatility spillovers. However, pr evious studies focus on the oil and clean energy market, and limited studies examine the market linkage. As a result, market linkage presents a challenge in front of energy transition into a green economy.Originality/valueThis paper makes a clear contribution to the literature by identifying the important existing and emerging research areas on the clean energy and fossil fuels market.
Mosque as a multi-functional public space destination: potential breathing space in dense urban fabrics of Hail City, Saudi Arabia
Authors: Alnaim Mohammed M. , Noaime Emad 
Published: October 2022

PurposeThe general concept of public space in Saudi Arabia is relatively recent; it is the result of an attempt to modernize several conservative traditions and norms, as well as the ambitious vision of 2030, which reprioritized Saudi cities' public spaces by implementing a quality-of-life improvement program. This study aims to investigate the relationship between mosque location and surrounding urban context as a significant urban design issue in order to comprehend the potential for transforming such an element into a multifunctional public space destination in a dense urban fabric.Design/methodology/approachThe study's objectives are divided into two areas: a spatial configuration analysis to examine the urban context and an urban analysis of the mosque site with a study area boundary of a 400-m buffer zone. Quantitative and qualitative methodologies were utilized; in addition, a user feedback survey was conducted in which several key insights were compiled, such as the importance of safety urban element factors, aesthetic elements and green areas in the built environment in making mosques places that are active public spaces.FindingsThe study’s main finding encourages governmental institutions to change their mindset from “open space around the mosque” to “the mosque as an element within public space,” which will have a significant impact on how the authors approach, improve and redevelop existing mosque locations. Several concepts were presented that should be reconsidered and redeveloped in order to align with the most recent sustainable urban design tools; the mosque to be overlooked as a community gathering place rather than just a place for religious rituals.Research limitations/implicationsThis study is limited to sacred mosque sites primarily found in Islamic countries; it focuses on Saudi Arabian urban and planning practices in relation to sociocultural norms. The authors believe that increasing the number of study samples or broadening the scope of the study to include other Saudi regions or other Middle Eastern countries could yield new findings and insights.Originality/valueThe study offers a conceptual design model based on survey results and qualitative analysis and recommending strategies that planners, designers and policymakers can use to design successful mosque places and pedestrian-oriented developments.
Impact of COVID‐19 inequalities on children: An intersectional analysis
Authors: Lemkow–Tovías Gabriel , Lemkow Louis , Cash‐Gibson Lucinda , Teixidó‐Compañó Ester , Benach Joan 
Published: October 2022

AbstractSocietal concerns about the effects of the COVID‐19 pandemic have largely focussed on the social groups most directly affected, such as the elderly and health workers. However, less focus has been placed on understanding the effects on other collectives, such as children. While children’s physical health appears to be less affected than the adult population, their mental health, learning and wellbeing is likely to have been significantly negatively affected during the pandemic due to the varying policy restrictions, such as withdrawal from face to face schooling, limited peer‐to‐peer interactions and mobility and increased exposure to the digital world amongst other things. Children from vulnerable social backgrounds, and especially girls, will be most negatively affected by the impact of COVID‐19, given their different intersecting realities and the power structures already negatively affecting them. To strengthen the understanding of the social determinants of the COVID‐19 crisis that unequally influence children’s health and wellbeing, this article presents a conceptual framework that considers the multiple axes of inequalities and power relations. This understanding can then be used to inform analyses and impact assessments, and in turn inform the development of effective and equitable mitigation strategies as well as assist to be better prepared for future pandemics.
Nonthermal pasteurization of pineapple juice: A review on the potential of achieving microbial safety and enzymatic stability
Authors: Shaik Lubna , Chakraborty Snehasis 
Published: October 2022

AbstractPineapple juice is preferred by consumers for its unique aroma and flavor that come from a set of amino acids, amines, phenolic compounds, and furanone. The juice is susceptible to spoilage, and a common practice is to pasteurize it at 70–95°C for 0.5–5 min. However, the characteristic flavors and phytochemicals are negatively influenced by the intense time–temperature treatment. To retain the thermosensitive compounds in the juice, some nonthermal technologies such as high‐pressure processing, pulsed electric field, pulsed light, ultrasound, and ultraviolet treatments have been explored. These techniques ensured microbial safety (5‐log reduction inE. coli,S. Typhimurium, orS. cerevisiae) while preserving a maximum ascorbic acid (84–99%) in the juice. The shelf life of these nonthermally treated juice varied between 14 days (UV treated at 7.5 mJ/cm2) and 6 months (clarified through microfiltration). Moreover, the inactivation of spoilage enzyme in the juice required a higher intensity. The present review discusses the potential of several nonthermal techniques employed for the pasteurization of pineapple juice. The pasteurization ability of the combined hurdle between mild thermal and nonthermal processing is also presented. The review also summarizes the target for pasteurization, the plan to design a nonthermal processing intensity, and the consumer perspective toward nonthermally treated pineapple juice. The techniques are compared on the common ground like safety, stability, and quality of the juice. This will help readers to select an appropriate nonthermal technology for pineapple juice production and design the intensity required to satisfy the manufacturers, retailers, and consumers.
Novel isochoric impregnation to develop high‐quality and nutritionally fortified plant materials (apples and sweet potatoes)
Authors: Bilbao‐Sainz Cristina , Chiou Bor‐Sen , Takeoka Gary , Williams Tina , Wood Delilah , Powell‐Palm Matthew J. , Rubinsky Boris , McHugh Tara 
Published: October 2022

AbstractIsochoric impregnation was explored as a novel pressure‐assisted infusion technique to fortify plant materials with bioactive compounds. Apple and potato cylinders were impregnated with a sucrose solution containing 4% ascorbic acid (AA) while freezing under isochoric conditions. Isochoric impregnation resulted in greater infusion of AA compared to infusion at atmospheric pressure, which demonstrated the feasibility of this impregnation technology. Processing temperatures (–3°C and –5°C) and processing times (1, 3, and 5 h) significantly affected the AA infusion. The AA content values ranged from 446 to 516 mg/100 g for apples and 322 to 831 mg/100 g for sweet potatoes under isochoric conditions. For both plant materials, isochoric impregnation at –3°C did not cause major changes in texture and microstructure of the biological tissues. These results indicated that isochoric impregnation of solid foods could be a feasible technology for infusion of bioactive compounds without significantly altering their matrix.Practical ApplicationThe findings of this study showed that the use of isochoric impregnation as a fortification technique is a promising way to develop fresh‐like and value‐added products with improved nutrition during preservation at subfreezing temperatures.
Gestational diabetes mellitus in the COVID‐19 pandemic: A retrospective study from Hangzhou, China
Authors: Yin Binbin , Wu Kaiqi , Hu Lingwei , Zheng Wanlu , Zheng Yidan , Duan Xiuzhi , Zhu Bo 
Published: October 2022

AbstractAimsOur study aimed to investigate changes in the prevalence of gestational diabetes mellitus (GDM) in the COVID‐19 pandemic and postpandemic era and the risk of adverse pregnancy outcomes in pregnant women diagnosed with GDM during the blockade period.MethodsFirst, we investigated changes in the prevalence of GDM and the population undergoing oral glucose tolerance tests (OGTT) after the COVID‐19 pandemic. We then collected clinical information from pregnant women diagnosed with GDM to explore the risk of adverse pregnancy outcomes in pregnant women with GDM during the COVID‐19 pandemic.ResultsAfter the COVID‐19 pandemic, the proportion of pregnant women in the total number of outpatient OGTT tests decreased yearly. The ratio was 81.30%, 79.71%, and 75.48% from 2019 to 2021, respectively, with the highest proportion of pregnant women in February 2020 (92.03%). The prevalence of GDM was higher in March 2020 compared to the same period in 2019. However, from 2019 to 2021, the prevalence decreased year by year with 21.46%, 19.81%, and 18.48%, respectively. The risk of adverse pregnancy outcomes for pregnant women diagnosed with GDM during the most severe period of the COVID‐19 pandemic did not differ from before the COVID‐19 pandemic.ConclusionsAfter the COVID‐19 pandemic, the prevalence of GDM increased during the most severe period of the epidemic, but the overall prevalence of GDM decreased year by year. In addition, the pandemic did not change the risk of adverse pregnancy outcomes in pregnant women with GDM.
Riboflavin depletion promotes longevity and metabolic hormesis in Caenorhabditis elegans
Authors: Yerevanian Armen , Murphy Luke M. , Emans Sinclair , Zhou Yifei , Ahsan Fasih M. , Baker Daniel , Li Sainan , Adedoja Adebanjo , Cedillo Lucydalila , Stuhr Nicole L. , Gnanatheepam Einstein , Dao Khoi , Jain Mohit , Curran Sean P. , Georgakoudi Irene , Soukas Alexander A. 
Published: October 2022

AbstractRiboflavin is an essential cofactor in many enzymatic processes and in the production of flavin adenine dinucleotide (FAD). Here, we report that the partial depletion of riboflavin through knockdown of the C. elegans riboflavin transporter 1 (rft‐1) promotes metabolic health by reducing intracellular flavin concentrations. Knockdown of rft‐1 significantly increases lifespan in a manner dependent upon AMP‐activated protein kinase (AMPK)/aak‐2, the mitochondrial unfolded protein response, and FOXO/daf‐16. Riboflavin depletion promotes altered energetic and redox states and increases adiposity, independent of lifespan genetic dependencies. Riboflavin‐depleted animals also exhibit the activation of caloric restriction reporters without any reduction in caloric intake. Our findings indicate that riboflavin depletion activates an integrated hormetic response that promotes lifespan and healthspan in C. elegans.
Managers' equity‐based compensation and soft‐talk management cash flow forecasts
Authors: Wang Weixiao , Zhang Lijuan 
Published: October 2022

AbstractWe investigate whether managers use their discretion in cash flow forecasts to influence the value of their equity‐based compensation. We find that managers are more likely to issue soft‐talk cash flow forecasts when their equity‐based compensation is higher. This association is affected by the news in management earnings forecasts and the propensity of accrual‐based earnings management. Further analyses show that the documented association is less pronounced after the Securities and Exchange Commission issued Regulation G, for firms with chief executive officers who are older or in their later tenure. Our results are robust to addressing potential correlated omitted variables bias.
Share pledge risk and government bailout fund
Authors: Hong Xin , Hu Yang , Zhang Wanting 
Published: October 2022

AbstractThe effects of government bailouts have been extensively examined in the literature. This paper is one of the first to empirically examine the bailout funds established in China in 2018, which aimed to mitigate firms' liquidity squeeze risk caused by share pledges. Utilising a hand‐collected dataset of government bailout funds, this paper finds that stock prices increased when government introduced the bailout fund plan, especially for non‐state‐owned enterprises (SOEs) and firms with small size and share pledges. Long‐term performance of bailed out firms increased as well. Overall, our results provide new evidence that government bailouts have positive effects on financial markets.
Exploring spatio‐temporal trends and environmental drivers of wildfire occurrence and impacts in Côte d'Ivoire, West Africa
Authors: Kouassi Jean‐Luc , Wandan Narcisse , Mbow Cheikh 
Published: October 2022

AbstractWildfires are a significant threat to environmental, social, economic and agricultural systems. This study investigated the spatiotemporal trends in wildfire activity and its interactions with climate and environmental factors in Côte d'Ivoire through remotely sensed MODIS data associated with climate, biophysical and anthropogenic data. We analysed relationships between wildfire occurrence and climate drivers using cross‐correlations, while Pearson chi‐squared and Kruskal–Wallis tests were selected to identify linkages between qualitative and quantitative environmental data, respectively. Seasonal Kendall and Sen's slope approaches were applied for trend analysis. During the period 2001–2019, 8150 wildfires were recorded annually, burning 2.69 million hectares per year, representing about 8.34% of Côte d'Ivoire. Fire detections and burnt areas highlighted a downward trend in all ecoregions with a predominance in the Sudanian zone. Wildfire occurrence showed a significant and strongly negative link with relative humidity and visibility, as well as a significant and positive correlation with maximum temperature, thermal amplitude and vapour‐pressure deficit. Also, the spatial distribution of wildfires was significantly conditioned by environmental factors. The findings of this study will help decision makers and managers to make decisions to reduce the vulnerability of local populations to current and future wildfire hazards.