bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nodulation potential and phenotypic diversity of rhizobia nodulating cowpea isolated from major growing areas of Ethiopia
Authors: Kebede Erana , Amsalu Berhanu , Argaw Anteneh , Tamiru Solomon 
Published: September 2022

AbstractRhizobia are legume specific, and they need to be characterized by different methods. This study assessed the nodulation potential and phenotypic diversity of rhizobia nodulating cowpea [Vigna unguiculata (L.) Walp.] isolated from major growing areas of Ethiopia. From 30 soil samples, 28 (93.3%) samples induced nodulation. Twenty‐eight isolates obtained from nodulated plants were evaluated using presumptive tests and different phenotypic characteristics. On yeast extract mannitol agar (YEMA) containing Congo red (CR), the isolates produced colony colors that were whitish to pale pink. All isolates showed no growth on peptone glucose agar and the isolates were capable of growing on ketolactose medium but did not turn the media color to yellow. All the isolates were also confirmed to be gram‐negative, rod‐shaped, and nonspore‐forming. The colony texture study revealed that 78.57% of the isolates had a large mucoid colony, whereas 21.43% exhibited a large watery colony texture with diameters ranging between 1 and 5 mm. Depending on the colony color on YEMA containing bromothymol blue, 22 (78.57%) isolates were slow growing, whereas six (21.43%) isolates were fast‐growing rhizobia. The cluster analysis based on phenotypic characteristics revealed a grouping of the isolates into two clusters at a similarity level of 75% and six further subclusters at a similarity level of ∼91%, indicating variations and diversity among the isolates. Overall, this study revealed significant variations among the cowpea nodulating rhizobial isolates and suggested that the soils of Ethiopia harbor populations of phenotypically diverse isolates. A further test to confirm these findings using genetic characterization and inclusion of reference strain is highly recommended.
Remote sensing evaluation of winter cover crop springtime performance and the impact of delayed termination
Authors: Thieme Alison , Hively W. Dean , Gao Feng , Jennewein Jyoti , Mirsky Steven , Soroka Alexander , Keppler Jason , Bradley Dawn , Skakun Sergii , McCarty Gregory W. 
Published: September 2022

AbstractIn 2019, the Maryland Department of Agriculture's Winter Cover Crop Program introduced a delayed termination incentive (after May 1) to promote springtime biomass accumulation. We used satellite imagery calibrated with springtime in situ measurements collected from 2006–2021 (n = 722) to derive biomass estimates for Maryland fields planted to cereal cover crop species (286,200 ha total over two seasons). Cover crop C content remained steady throughout the cover crop growing season (42.6% of biomass), whereas N concentration had an inverse relationship with biomass and ranged from 1.7 to 2.9%. Throughout Maryland, delayed termination fields (n = 19,120; average termination of May 18) were, on average, estimated to accumulate an additional 789 kg of biomass, 15 kg of N, and 336 kg of C per hectare when compared to fields associated with standard termination dates (n = 28,811; average termination of April 16). Over two cover crop seasons (2019–2021), the delayed termination incentive yielded an extra 75,660,000 kg biomass, 1,526,000 kg N, and 32,230,000 kg C across 96,040 hectares. Regularly terminated field incentives cost an average of US$0.10 per kg of biomass and $4.09 per kg of N, with variability associated with agronomic management (species, planting method). Delayed termination fields cost of $0.08 per kg of biomass and $3.51 per kg of N. Late‐planted cover crops that were terminated early had minimal environmental benefit, and wheat, which comprised 68% of cover crop area, performed poorly compared with other cereal species. Our findings demonstrate that substantial additional springtime biomass, C, and N accumulation were achieved through the delayed termination incentive.
Effect of mutation allele frequency on the risk stratification of myelodysplastic syndrome patients
Authors: Lee Wan‐Hsuan , Lin Chien‐Chin , Tsai Cheng‐Hong , Tseng Mei‐Hsuan , Kuo Yuan‐Yeh , Liu Ming‐Chih , Tang Jih‐Luh , Sun Hsun‐I , Chuang Yi‐Kuang , Chou Wen‐Chien , Hou Hsin‐An , Tien Hwei‐Fang 
Published: September 2022

AbstractMyelodysplastic syndrome (MDS) is a heterogeneous group of clonal myeloid malignancies. Though several recurrent mutations are closely correlated with clinical outcomes, data concerning the association between mutation variant allele frequencies (VAF) and prognosis are limited. In this study, we performed comprehensive VAF analyses of relevant myeloid‐malignancy related mutations in 698 MDS patients and correlated the results with their prognosis. Mutation VAF in DNMT3A, TET2, ASXL1, EZH2, SETBP1, BCOR, SFSF2, ZRSR2, and TP53 mutations correlated with outcomes. In multivariable analysis, DNMT3A and ZRSR2 mutations with high VAF and mutant IDH2, CBL, U2AF1, and TP53 were independent poor prognostic factors for overall survival. A substantial portion of patients in each revised International Prognostic Scoring System (IPSS‐R) risk group could be adjusted to different prognostic groups based on the integrated VAF and mutational profiles. Patients with these unfavorable mutations in each IPSS‐R risk subgroup had survivals worse than other patients of the same risk but similar to those in the next higher‐risk subgroup. Furthermore, patients harboring U2AF1 mutation might benefit from hypomethylating agents. This study demonstrated the critical role of VAF of mutations for risk stratification in MDS patients and may be incorporated in novel scoring systems.
Development of a Catalytic Ester Activation Protocol for the Efficient Formation of Amide Bonds using an Ar−I/HF⋅pyridine/ mCPBA System
Authors: Lee Hyo‐Jun , Choi Eun‐Sol , Maruoka Keiji 
Published: September 2022

AbstractA new catalytic approach for the activation of esters, that possess a p‐hydroxyphenyl group as a potential activating group on the ester residue, was developed and applied to the efficient synthesis of amides and peptides. A characteristic feature of this protocol is the in‐situ generation of a hypervalent iodine(III) species using an Ar−I/HF⋅pyridine/mCPBA system. A reactive acyl fluoride intermediate is smoothly generated via the oxidative dearomatization of phenols in the presence of a catalytic amount of 4‐iodoanisole, thereby affording various amides and peptides including sterically hindered derivatives.
Recent Advances in the Asymmetric Total Synthesis of Camptothecin
Authors: Song Liangliang , Lv Zhenwei , Zhang Kui , Wu Yile , Van der Eycken Erik V. , Cai Lingchao 
Published: September 2022

AbstractDuring the past decades, a variety of routes for the total synthesis of camptothecin have been reported. Considering only (S)‐camptothecin and (S)‐camptothecin analogues possessing the ability to inhibit DNA enzyme topoisomerase I, numerous advances have been achieved for the asymmetric total synthesis of camptothecin, providing concise and scalable approaches to (S)‐camptothecin for the academic community and pharmaceutical industry. This review outlines recent advances in the asymmetric total synthesis of camptothecin, and is timely and highly desirable for the rapid development of this field. By constructing the chiral tertiary alcohol center, diverse attractive strategies have been developed for the total synthesis of (S)‐camptothecin.
Survey of Glasgow Coma Scale and PaO2/FIO2 ratio assessment methods for the Sequential Organ Failure Assessment score in Japanese intensive care units
Authors: Tamoto Mitsuhiro , Imai Takumi , Aida Rei , Harada Yusuke , Wakabayashi Yuki , Satone Gaku , Ichoda Shunsuke , Unoki Takeshi , Shintani Ayumi 
Published: September 2022

AimAccurately calculating the Sequential Organ Failure Assessment (SOFA) score is essential for medical resource allocation and decision‐making. This study surveyed Japanese intensive care units regarding their assessment of the Glasgow Coma Scale (GCS) and PaO2/FIO2 ratio, components of the SOFA score.MethodsA cross‐sectional, web‐based survey was conducted among healthcare workers. The survey consisted of questions about the intensive care units where they work and questions for respondents. It was distributed to healthcare workers by e‐mail through the Japanese Society of Intensive Care Medicine mailing list and social networking service.ResultsAmong 414 responses, we obtained 211 valid responses and 175 survey results from unique intensive care units. When assessing GCS in patients under the influence of sedatives, 45.1% (95% confidence interval, 37.6–52.8) of intensive care units assessed GCS assuming that the sedatives had no influence. For the PaO2/FIO2 ratio in the SOFA score, calculation based on the Japanese Intensive Care Patient Database definition document and mechanical ventilator settings were the most common methods in patients with oxygen masks and on extracorporeal membrane oxygenation, respectively. Approximately 60% of respondents indicated that it was difficult to assess GCS assuming that sedatives had no influence.ConclusionIn patients under the influence of sedatives, approximately half of the intensive care units assessed assumed GCS. There was variation in the methods used to assess the PaO2/FIO2 ratio. Standardized assessment methods for GCS and the PaO2/FIO2 ratio are needed to obtain valid SOFA score.
Organogold(III) Complexes Display Conditional Photoactivities: Evolving From Photodynamic into Photoactivated Chemotherapy in Response to O2Consumption for Robust Cancer Therapy
Authors: Luo Yunli , Cao Bei , Zhong Mingjie , Liu Moyi , Xiong Xiaolin , Zou Taotao 
Published: September 2022

AbstractPhotodynamic therapy (PDT) is a spatiotemporally controllable, powerful approach in combating cancers but suffers from low activity under hypoxia, whereas photoactivated chemotherapy (PACT) operates in an O2‐independent manner but compromises the ability to harness O2for potent photosensitization. Herein we report that cyclometalated gold(III)‐alkyne complexes display a PDT‐to‐PACT evolving photoactivity for efficient cancer treatment. On the one hand, the gold(III) complexes can act as dual photosensitizers and substrates, leading to conditional PDT activity in oxygenated condition that progresses to highly efficient PACT (ϕ up to 0.63) when O2is depleted in solution and under cellular environment. On the other hand, the conditional PDT‐to‐PACT reactivity can be triggered by external photosensitizers in a similar manner in vitro and in vivo, giving additional tumor‐selectivity and/or deep tissue penetration by red‐light irradiation that leads to robust anticancer efficacy.
Mechanical loading of graphene quantum dots on Staphylococcus aureus surface protein G: A molecular dynamics study
Authors: Mousanezhad Sedigheh , Davoodi Jamal 
Published: September 2022

AbstractThis research studies graphene quantum dots (GQDs) adsorption on Staphylococcus aureus surface protein G (SasG) using the molecular dynamics simulation method that in addition to investigating various aspects of adsorption, including edge and surface effects of GQDs, and effects of orientation and size of GQDs, proposes a new study method in edge effects studies. Through our simulations to find edge effects, we found that even if GQD approaches the protein with its edges, GQDs' edges are not freely adsorbed on the protein because when the particles interact with each other, GQD rotates and then adsorbs the protein with its surface; therefore, GQD having initial velocity was applied to the protein to force the protein to interact with GQDs' edges to find both surface and edge effects. This is a new way of investigating that we propose to study edge effects in the molecular dynamics simulation method.
Impact of microcarrier concentration on mesenchymal stem cell growth and death: Experiments and modeling
Authors: Maillot Charlotte , De Isla Natalia , Loubiere Celine , Toye Dominique , Olmos Eric 
Published: September 2022

AbstractMesenchymal stem cell (MSC) products are promising therapeutic candidates to treat a wide range of pathologies. The successful commercialization of these cell therapies will, however, depend on the development of reproducible cell production processes. For this, using microcarriers as growth supports within controlled conditions may be a viable process option. Although increasing microcarrier concentration may be associated with greater productivity due to the increased available culture surface, additional friction or shocks between microcarriers are likely to lead to undesired cell death. However, data detailing the impact of microcarrier collisions on MSC growth remains scarce. The following work demonstrates that MSC growth on microcarriers is greatly influenced by particle concentration even when little impact is observed on the apparent growth rate. It is suggested that the apparent growth rate may result in an equilibrium between growth and death kinetics which are independently affected by particle concentration and that certain MSC quality attributes may be progressively degraded in parallel. In addition, the theoretical reduction of the MSC growth rate was modeled according to the ratio between the average interparticle distance and the Kolmogorov scale. This study is an original contribution toward understanding the hydrodynamic effects in microcarrier‐based stem cell cultures.
High‐throughput screening of optimal process conditions using model predictive control
Authors: Krausch Niels , Kim Jong Woo , Barz Tilman , Lucia Sergio , Groß Sebastian , Huber Matthias C. , Schiller Stefan M. , Neubauer Peter , Cruz Bournazou Mariano N. 
Published: September 2022

AbstractModern biotechnological laboratories are equipped with advanced parallel mini‐bioreactor facilities that can perform sophisticated cultivation strategies (e.g., fed‐batch or continuous) and generate significant amounts of measurement data. These systems require not only optimal experimental designs that find the best conditions in very large design spaces, but also algorithms that manage to operate a large number of different cultivations in parallel within a well‐defined and tightly constrained operating regime. Existing advanced process control algorithms have to be tailored to tackle the specific issues of such facilities such as: a very complex biological system, constant changes in the metabolic activity and phenotypes, shifts of pH and/or temperature, and metabolic switches, to name a few. In this study we implement a model predictive control (MPC) framework to demonstrate: (1) the challenges in terms of mathematical model structure, state, and parameter estimation, and optimization under highly nonlinear and stiff dynamics in biological systems, (2) the adaptations required to enable the application of MPC in high throughput bioprocess development, and (3) the added value of MPC implementations when operating parallel mini‐bioreactors aiming to maximize the biomass concentration while coping with hard constrains on the dissolved oxygen tension profile.
Bilateral expansion of mandible and maxillae in a 5‐year‐old girl
Authors: Movahedi Nasim , Malek Abdolreza , Ziaee Vahid 
Published: September 2022

AbstractA 5‐year‐old girl with symmetrical expansion of mandible and both maxillae, lack of eruption of several teeth an hypertrophied palate was presented to our clinic. upon detailed laboratory, imaging and histopathological evaluation, the diagnosis of “Cherubism” a rare autoinflammatory bone disease was confirmed.
Sequence Selective Ring‐opening Terpolymerisation Facilitates Higher Order Switchable Catalysis
Authors: Plajer Alex J. 
Published: September 2022

AbstractSequence selective ring‐opening terpolymerisation (ROTERP) allows the design of block polymerisation cascades with three distinct mechanistic cycles. Ring‐opening polymerisation (ROP), ring‐opening copolymerisation (ROCOP) and ROTERP can be combined in cascades where the onset of the most complex process supresses the occurrence of the simpler ones. The cascades furthermore enable the incorporation of different heteroallenes between blocks.
Influence of Lithium Difluoro (Oxalato) Borate Additive on the Performance of LiCoPO4−LiFePO4 Solid‐Solution by Carbothermal Reduction
Authors: Sreedeep Sreekumar , Praneetha Selvarasu , Lee Yun‐Sung , Aravindan Vanchiappan 
Published: September 2022

AbstractWe report the effect of lithium difluoro (oxalato) borate (LiDFOB) additive on the electrochemical performance of LiFe0.5Co0.5PO4@C, along with the fabrication of a full‐cell with Li4Ti5O12 (LTO) anode. Here, we used a scalable carbothermal reduction for the synthesis of LiCoPO4−LiFePO4 solid‐solution, LiFe0.5Co0.5PO4@C. The electrochemical activity of LiFe0.5Co0.5PO4@C cathode is studied by varying LiDFOB (up to 2 wt%) concentration and showed significant improvement compared to the normal electrolyte in half‐cell assembly. Among the various concentrations of LiDFOB, 1.5 and 2 wt% additions are optimized owing to the higher discharge capacity of 114 and 116 mAh g−1 with a capacity retention of 65 and 73 % after 60 cycles, respectively. The Li+ ion diffusion coefficients are calculated from both cyclic voltammetry and impedance spectroscopy analysis and show a decrease in the value as the concentration of LiDFOB is increased from 0.5 to 2 wt%, with an order of magnitude in the range of ∼10−14 cm2 s−1. The full‐cell, LiFe0.5Co0.5PO4@C/LTO is also fabricated and displayed a discharge capacity of 95 mAh g−1. The possibility of using such full‐cell assembly with various temperature conditions is studied from −10 to 25 °C.
Influence of Chloride and Nitrate Anions on Copper Electrodeposition onto Au(111) from Deep Eutectic Solvents
Authors: Geng Tanja , Schick Benjamin W. , Uhl Matthias , Kuehne Alexander J. C. , Kibler Ludwig A. , Ceblin Maximilian U. , Jacob Timo 
Published: September 2022

AbstractInvited for this issue's Front Cover is the group of Prof. Timo Jacob in collaboration with Prof. Alexander Kuehne, both from Ulm University (Germany). The cover picture shows copper electrodeposition on a gold‐coated quartz crystal from copper(II) chloride in a choline chloride/ethylene glycol deep eutectic solvent (DES), as monitored by electrochemical quartz crystal microbalance (EQCM). Read the full text of the Research Article at 10.1002/celc.202101263.
Atezolizumab plus bevacizumab versus nivolumab as first‐line treatment for advanced or unresectable hepatocellular carcinoma: A cost‐effectiveness analysis
Authors: Li Yan , Liang Xueyan , Li Huijuan , Chen Xiaoyu 
Published: September 2022

BackgroundThe cost effectiveness of atezolizumab plus bevacizumab (atezo‐beva) versus nivolumab treatment for advanced or unresectable hepatocellular carcinoma is still uncertain. In this study, the cost effectiveness of these treatments was assessed in the United States.MethodsA cost‐effectiveness analysis integrating a network meta‐analysis framework was performed using data from the IMbrave150 (ClinicalTrials.gov identifier NCT03434379) and CheckMate 459 (ClinicalTrials.gov identifier NCT02576509) trials. In total, 1244 patients were enrolled. A partitioned survival model was used to evaluate cost effectiveness. A deterministic one‐way sensitivity analysis and probabilistic sensitivity analyses were further performed to evaluate model robustness. Subgroup analyses were also performed.ResultsCompared with the outcomes using nivolumab, the hazard ratio (HR) for overall survival with atezo‐beva was 0.68 (95% CI, 0.48–0.98), and the HR for progression‐free survival was 0.63 (95% CI, 0.47–0.85). Atezo‐beva treatment was associated with an increase of 1.13 life‐years and an increase of 0.69 quality‐adjusted life‐years (QALYs), as well as a $78,280 increase in cost per patient. The incremental cost‐effectiveness ratio was $113,892 per QALY. The incremental net health benefit and the incremental net monetary benefit were 0.17 QALYs and $24,770, respectively, at a willingness‐to‐pay (WTP) threshold of $150,000 per QALY. The model was most sensitive to the HR for progression‐free survival. The probability of atezo‐beva being considered cost effective was 78%, and it was >50% in most of the subgroups at the WTP threshold of $150,000 per QALY.ConclusionsAt a WTP threshold of $150,000 per QALY and under current drug pricing, atezo‐beva is likely considered cost‐effective as a first‐line treatment for advanced or unresectable hepatocellular carcinoma compared with nivolumab.
A high step‐up isolated DC–DC converter based on voltage multiplier cell
Authors: Andres Bernardo , Romitti Leonardo , Dupont Fabrício H. , Roggia Leandro , Schuch Luciano 
Published: September 2022

AbstractGenerating sources of renewable systems, like photovoltaic module or fuel cell, have a low‐output voltage that has to be boosted for most of applications, such as grid‐tie inverters. To accomplish this, an isolated DC–DC high step‐up single‐ended primary‐inductor converter (SEPIC) with a Greinacher voltage doubler cell is presented. It has the advantage of continuous input current, high efficiency, and high‐voltage gain and isolation and demands a single switch, being suitable for low‐power grid‐tie photovoltaic systems. The operating principles and steady‐state analysis are presented, including the detailed analysis of resonant stage, and the effects of transformer winding capacitances on converter operation are investigated. Moreover, the effects of resonance frequency variations on converter efficiency are experimentally investigated. Experimental results on a 50 kHz and 200 W prototype are presented to validate the proposed concept.
Advanced biomaterials for periodontal tissue regeneration
Authors: Daghrery Arwa , Bottino Marco C. 
Published: September 2022

SummaryThe periodontium is a suitable target for regenerative intervention, since it does not functionally restore itself after disease. Importantly, the limited regeneration capacity of the periodontium could be improved with the development of novel biomaterials and therapeutic strategies. Of note, the regenerative potential of the periodontium depends not only on its tissue‐specific architecture and function, but also on its ability to reconstruct distinct tissues and tissue interfaces, suggesting that the advancement of tissue engineering approaches can ultimately offer new perspectives to promote the organized reconstruction of soft and hard periodontal tissues. Here, we discuss material‐based, biologically active cues, and the application of innovative biofabrication technologies to regenerate the multiple tissues that comprise the periodontium.