bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ichnoassemblages from the Wilcox Formation in the Burgos Basin, northeastern Mexico
Authors: Hernández-Ocaña María I. , Torres de la Cruz Felipe , Chacón Baca Elizabeth , Eguiluz de Antuñano Samuel , Chávez-Cabello Gabriel 
Published: June 2022

Abstract

An ichnological analysis of the Paleocene–Eocene Wilcox Formation at San José de la Laja and La Curva localities within the Burgos Basin in northeastern Mexico revealed seven ichnogenera:
Chondrites
,
Diplocraterion
,
Ophiomorpha
,
Palaeophycus
,
Planolites
,
Sinusichnus
and
Skolithos
. The trace fossils were grouped into different ichnoassemblages, including a detailed facies analysis. These results show their apparent association with the
Skolithos
and
Cruziana
Ichnofacies in the two studied localities. The integrative analysis of trace fossils and lithofacies made it possible to infer plausible depositional environments for registered periods of fair-weather conditions in both localities of the Burgos Basin, which represent ecosystems characterized by low diversity and abundance. In San José de La Laja, the environment varies from storm affected to tidal setting. In La Curva, lithofacies and ichnology are modulated by storm events in a transitional shoreface, coastal bar and intertidal setting within a deltaic plain. Both localities indicate a shelf impacted by deltaic processes and storm events. Living conditions for most endobenthic organisms were harsh. These results support a long-term regression of the coastal zone during the Paleogene–Neogene and suggest a palaeoenvironment similar to this in the south of Texas during the Paleogene.
Identification of Two Genetic Loci Associated with Leukopenia after Chemotherapy in Patients with Breast Cancer
Authors: Fasching Peter A. , Liu Duan , Scully Steve , Ingle James N. , Lyra Paulo C. , Rack Brigitte , Hein Alexander , Ekici Arif B. , Reis Andre , Schneeweiss Andreas , Tesch Hans , Fehm Tanja N. , Heinrich Georg , Beckmann Matthias W. , Ruebner Matthias , Huebner Hanna , Lambrechts Diether , Madden Ebony , Shen Jess , Romm Jane , Doheny Kim , Jenkins Gregory D. , Carlson Erin E. , Li Liang , Fridley Brooke L. , Cunningham Julie M. , Janni Wolfgang , Monteiro Alvaro N.A. , Schaid Daniel J. , Häberle Lothar , Weinshilboum Richard M. , Wang Liewei 
Published: June 2022

Abstract

Purpose:
To identify molecular predictors of grade 3/4 neutropenic or leukopenic events (NLE) after chemotherapy using a genome-wide association study (GWAS).

Experimental Design:
A GWAS was performed on patients in the phase III chemotherapy study SUCCESS-A (n = 3,322). Genotyping was done using the Illumina HumanOmniExpress-12v1 array. Findings were functionally validated with cell culture models and the genotypes and gene expression of possible causative genes were correlated with clinical treatment response and prognostic outcomes.

Results:
One locus on chromosome 16 (rs4784750; NLRC5; P = 1.56E-8) and another locus on chromosome 13 (rs16972207; TNFSF13B; P = 3.42E-8) were identified at a genome-wide significance level. Functional validation revealed that expression of these two genes is altered by genotype-dependent and chemotherapy-dependent activity of two transcription factors. Genotypes also showed an association with disease-free survival in patients with an NLE.

Conclusions:
Two loci in NLRC5 and TNFSF13B are associated with NLEs. The involvement of the MHC I regulator NLRC5 implies the possible involvement of immuno-oncological pathways.
Single-Cell RNA Sequencing Reveals the Tissue Architecture in Human High-Grade Serous Ovarian Cancer
Authors: Xu Junfen , Fang Yifeng , Chen Kelie , Li Sen , Tang Sangsang , Ren Yan , Cen Yixuan , Fei Weidong , Zhang Bo , Shen Yuanming , Lu Weiguo 
Published: June 2022

Abstract

Purpose:
The heterogeneity of high-grade serous ovarian cancer (HGSOC) is not well studied, which severely hinders clinical treatment of HGSOC. Thus, it is necessary to characterize the heterogeneity of HGSOC within its tumor microenvironment (TME).

Experimental Design:
The tumors of 7 treatment-naïve patients with HGSOC at early or late stages and five age-matched nonmalignant ovarian samples were analyzed by deep single-cell RNA sequencing (scRNA-seq).

Results:
A total of 59,324 single cells obtained from HGSOC and nonmalignant ovarian tissues were sequenced by scRNA-seq. Among those cells, tumor cells were characterized by a set of epithelial-to-mesenchymal transition (EMT)-associated gene signatures, in which a combination of NOTCH1, SNAI2, TGFBR1, and WNT11 was further selected as a genetic panel to predict the poor outcomes of patients with HGSOC. Matrix cancer-associated fibroblasts (mCAF) expressing α-SMA, vimentin, COL3A, COL10A, and MMP11 were the dominant CAFs in HGSOC tumors and could induce EMT properties of ovarian cancer cells in the coculture system. Specific immune cell subsets such as C7-APOBEC3A M1 macrophages, CD8+ TRM, and TEX cells were preferentially enriched in early-stage tumors. In addition, an immune coinhibitory receptor TIGIT was highly expressed on CD8+ TEX cells and TIGIT blockade could significantly reduce ovarian cancer tumor growth in mouse models.

Conclusions:
Our transcriptomic results analyzed by scRNA-seq delineate an ecosystemic landscape of HGSOC at early or late stages with a focus on its heterogeneity with TME. The major applications of our findings are a four–EMT gene model for prediction of HGSOC patient outcomes, mCAFs’ capability of enhancing ovarian cancer cell invasion and potential therapeutic value of anti-TIGIT treatment.
A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear-Cell Renal Cell Carcinoma
Authors: Rappold Phillip M. , Vuong Lynda , Leibold Josef , Chakiryan Nicholas H. , Curry Michael , Kuo Fengshen , Sabio Erich , Jiang Hui , Nixon Briana G. , Liu Ming , Berglund Anders E. , Silagy Andrew W. , Mascareno Eduardo A. , Golkaram Mahdi , Marker Mahtab , Reising Albert , Savchenko Alexander , Millholland John , Chen Ying-Bei , Russo Paul , Coleman Jonathan , Reznik Ed , Manley Brandon J. , Ostrovnaya Irina , Makarov Vladimir , DiNatale Renzo G. , Blum Kyle A. , Ma Xiaoxiao , Chowell Diego , Li Ming O. , Solit David B. , Lowe Scott W. , Chan Timothy A. , Motzer Robert J. , Voss Martin H. , Hakimi A. Ari 
Published: June 2022

Abstract

It is poorly understood how the tumor immune microenvironment influences disease recurrence in localized clear-cell renal cell carcinoma (ccRCC). Here we performed whole-transcriptomic profiling of 236 tumors from patients assigned to the placebo-only arm of a randomized, adjuvant clinical trial for high-risk localized ccRCC. Unbiased pathway analysis identified myeloid-derived IL6 as a key mediator. Furthermore, a novel myeloid gene signature strongly correlated with disease recurrence and overall survival on uni- and multivariate analyses and is linked to TP53 inactivation across multiple data sets. Strikingly, effector T-cell gene signatures, infiltration patterns, and exhaustion markers were not associated with disease recurrence. Targeting immunosuppressive myeloid inflammation with an adenosine A2A receptor antagonist in a novel, immunocompetent, Tp53-inactivated mouse model significantly reduced metastatic development. Our findings suggest that myeloid inflammation promotes disease recurrence in ccRCC and is targetable as well as provide a potential biomarker-based framework for the design of future immuno-oncology trials in ccRCC.

Significance:
Improved understanding of factors that influence metastatic development in localized ccRCC is greatly needed to aid accurate prediction of disease recurrence, clinical decision-making, and future adjuvant clinical trial design. Our analysis implicates intratumoral myeloid inflammation as a key driver of metastasis in patients and a novel immunocompetent mouse model.
This article is highlighted in the In This Issue feature, p. 2221
Primary Kaposi’s Sarcoma of the Nasal Cavity: Clinical Experience and Review of the Literature
Authors: Xiang Peng , Liu Mengya , Lu Xueyan , Tang Wei , Liu Jin 
Published: June 2022

Kaposi’s sarcoma (KS) is a vascular sarcoma derived from vascular endothelial cells and presents with multiple lesions. It mainly appears on the skin and oral mucosa, usually in the face, oral mucosa, and genitals. Very few cases of primary lesions in the nasal cavity have been reported. It is often difficult to diagnose only by imaging examination. Here, we describe a case of KS in a patient who was human immunodeficiency virus (HIV)-negative, in which the primary sites were the nasal mucosa and nasal septum. A diagnosis was made according to the patient’s clinical presentation, physical examination, laboratory examination, imaging examination, and histopathological results. We used surgical resection combined with chemotherapy, with 6 months’ postoperative follow-up without recurrence. We reviewed the relevant literature to identify similar cases and summarize the findings reported on this rare manifestation of KS. We recommend that, where possible, antiviral therapy such as interferon, and regular review should continue, to improve the survival rate and patients’ quality of life.
Evaluating Social Determinants of Health Domains and Their Predictive Validity Within Black/African American and White Older Adults From the ACTIVE Trial
Authors: Clay Olivio J. , Ball Karlene K. , Wheeler Katie M. , Crowe Michael , Marsiske Michael , Dean Lorraine T. , Thorpe Roland J. , Jones Richard , Owens Joshua H. , Rebok George W. , Willis Sherry L. 
Published: June 2022

Objective To assess domains of social determinants of health (SDoH) and their associations with cognition and quality of life. Method This investigation uses baseline data from individuals participating in the ACTIVE trial ( n = 2505) to reproduce the SDoH domains described in Healthy People 2030 (economic stability, health care, education, neighborhood and built environment, and social and community context). Results: Results support using data from the ACTIVE trial to assess all five SDoH domains, and the ability of the composites to predict baseline performance on measures of cognition and self-reported quality of life within a sample of older adults. Additionally, higher SDoH domain scores were associated with better functioning on composite measures of cognition and higher scores for mental and general health-related quality of life with Access to Healthcare associated with all outcomes. Discussion: These findings can inform investigators interested in assessing multiple domains of SDoH and highlight the importance of access to health care within older Black/African American and White older adults.
A 12‐year retrospective review of tetrodotoxin poisoning in Hong Kong
Authors: Cheung Kent Shek , Chan Chi Keung 
Published: June 2022

Introduction:Tetrodotoxin poisoning is typically caused by puffer fish consumption. This study is to review its demographics, clinical presentations and management in Hong Kong.Method:Reported cases of tetrodotoxin poisoning to Hong Kong Poison Information Centre from October 2008 to March 2021 were included in the study. Information from electronic database was analysed.Results:Forty‐two cases were included in this case series. The number of incidents of tetrodotoxin poisoning ranged from 0 to 5 per year, mostly in the winter months. Median ingestion‐to‐symptom and ingestion‐to‐door time was 2 and 6.5 h, respectively. 48% of puffer fish meal sharer was symptomatic and attended emergency department. 100% and 48% of all cases had neurological and cardiovascular/gastrointestinal symptoms, respectively. Fukuda and Tani clinical Grade 1 and 2 were the most common presentation. 17% required intensive care while most patients were managed safely in emergency department and/or emergency medicine ward.Conclusion:There are recurrent incidents of tetrodotoxin poisoning in Hong Kong. Public education is the key to prevent future outbreaks. Targeted history and enhanced availability of tetrodotoxin test would assist in its diagnoses.
Developing the Florida Academic Cancer Center Alliance Health Disparities Common Measure: The Florida Health and Ancestry Survey
Authors: Dyal Brenda W. , Uscanga Zulema L. , Bailey Zinzi , Schmit Stephanie , Hoehn Alina , Garcia Jennifer , Gwede Clement K. , Brownstein Naomi , Powell-Roach Keesha , Johnson-Mallard Versie , Krieger Janice L. , Kobetz Erin , Vadaparampil Susan , Odedina Folakemi T. , Wilkie Diana J. 
Published: June 2022

Purpose Our specific aim was to develop and assess the consensus-based validity of common measures for understanding health behaviors and ancestry in Florida’s population subgroups and establish the feasibility of wide-scale implementation of the measures and biospecimen collection within three cancer centers’ catchment areas. Methods Using the National Cancer Institute’s Grid-Enabled Measures web-based platform and an iterative process, we developed the Florida Health and Ancestry Survey (FHAS). We then used three sampling approaches to implement the FHAS: community-engaged, panel respondent, and random digit dialing (RDD). We asked a subset of participants to provide a saliva sample for future validation of subjective ancestry report with DNA-derived ancestry markers. Results This process supported the FHAS content validity. As an indicator of feasibility, the goals for completed surveys by sampling approach were met for two of the three cancer centers, yielding a total of 1438 completed surveys. The RDD approach produced the most representative sample. The panel sampling approach produced inadequate representation of older individuals and males. The community-engaged approach along with social media recruitment produced extreme underrepresentation only for males. Two of the cancer centers mailed biospecimen kits, whereas one did not due to resource constraints. On average, the community engaged approach was more productive in obtaining returned biospecimen samples (80%) than the panel approach (48%). Conclusions We successfully developed and implemented the FHAS as a common measure to show its feasibility for understanding cancer health disparities in Florida. We identified sampling approach successes and challenges to obtaining biospecimens for ancestry research.
Anatomic acetabular reconstruction with femoral head autograft for developmental dysplasia of the hip (DDH) with a minimum follow-up of 10 years
Authors: Güneş Zirvecan , Bekmez Şenol , Çağlar Ömür , Mazhar Tokgözoğlu Ahmet , Atilla Bülent 
Published: June 2022

Background: DDH with coxarthrosis causes significant deformity and bone deficiency. Various reconstructive techniques have been proposed to treat developmental dysplasia of the hip. However, the existing literature has not yet reached a consensus on the best technique regarding long-term survival. Objectives: This study aims to evaluate the long-term survival of uncemented hydroxyapatite (HA) coated acetabular components augmented with a femoral head autograft. Methods: We retrospectively reviewed the cases of 31 hips in 29 patients (24 female, 5 male, mean age 45.06 years) treated with HA-coated cementless components and femoral head autograft between 2000-2008 with a minimum follow-up of 10 years. Graft resorption, cup loosening and the anatomical hip centre were determined. Functional outcomes were calculated using the Harris Hip Scoring system. The survival of the acetabular component was evaluated using the Kaplan-Meier method. Results: In 24 hips (77,4%), we reconstructed the hip centre anatomically. The remaining cups had variable deviations from the anatomical rotation centre. Only one patient required revision due to loosening. Survival analysis revealed 96.8% survival at 10 years. The mean Harris Hip Score was 39.23 preoperatively and 84.77 at final follow-up. There was no statistical correlation between revision and any of the measured parameters. Discussion: Acetabular reconstruction with a femoral head autograft allows for anatomical cup positioning, early structural support and increases bone stock for future revisions. Although our prior cemented cup study showed that anatomical cup orientation is critical, this study demonstrated the absence of a correlation between implant failure and cup positioning, suggesting that HA-coated cementless cups are more stable and forgiving. Conclusions: HA-coated acetabular cups augmented with femoral head autograft provided long-term, reliable and durable cup fixation in dysplastic hips of young adults.
Time to Return to Work After an Occupational Injury and Its Prognostic factors Among Employees of Large-Scale Metal Manufacturing Facilities in Ethiopia: A Retrospective Cohort
Authors: Tamene Aiggan , Habte Aklilu , Derilo Habtamu Tamrat , Endale Fitsum , Gizachew Addisalem , Sulamo Dawit , Afework Abel 
Published: June 2022

Background: Globally, occupational risk factors are thought to be responsible for at least 1.9 million deaths and 90 million disability-adjusted life years per year. Occupational injury survivorship has increased in Ethiopia in recent years. However, the vast majority of the victims are young people who are impacted in their everyday life as a result of occupational injuries. While research in developed countries has revealed several factors related to early return to work, there have been very few studies of significance in underdeveloped countries, including Ethiopia. Methods: Metalworkers who had an occupational accident between January 1, 2017, and December 31, 2021, were investigated in a facility-based retrospective cohort. Data was collected from 422 medical records and registration books using a standardized abstraction tool. STATA 15 was used to analyze the data. The median time it took to return to work was computed. The Kaplan Meier survival curve was used to estimate the time to return to work across covariates. A multivariable Cox proportional hazard model was used to identify statistically significant predictors of return to work. Results: After a median of 45 days away from work, 310 of the 422 (73.5%) cases returned to work (95% CI 39.7-50.2). The total incidence density of return to work was 1.21 (95% CI = 1.01-1.30) per 100 person-days observed. Professional certification (AHR: 2.15, 95% CI: 1.62-2.87), working as a rigger (AHR: 1.59, 95% CI 1.20-2.10), having dependents at home (AHR = 1.59, 95% CI = 1.09-2.64), and injuries caused by body movement without any physical stress (AHR = 2.61, 95% CI = 1.92-3.56) were all associated with return to work. Conclusion: Return to work is influenced by a range of factors other than the type or severity of the injury incurred. Multidisciplinary approaches such as clinical treatment and rehabilitation, ergonomics interventions, and economic and social assistance should be prioritized in the efforts to aid employees’ return to work.
Effect of sodium-glucose co-transporter-2 inhibitors on arterial stiffness: A systematic review and meta-analysis of randomized controlled trials
Authors: Patoulias Dimitrios , Papadopoulos Christodoulos , Kassimis George , Fragakis Nikolaos , Vassilikos Vassilios , Karagiannis Asterios , Doumas Michael 
Published: June 2022

Background: Arterial stiffness represents an established cardiovascular risk marker. Sodium-glucose co-transporter-2 (SGLT-2) inhibitors have significant cardio-protective effects. Herein we sought to determine the effect of SGLT-2 inhibitors on pulse wave velocity (PWV). Methods: We searched PubMed, Cochrane Library, and grey literature from inception to 7th February 2022 for randomized controlled trials (RCTs) enrolling adult subjects with or without type 2 diabetes mellitus (T2DM), assigned to a SGLT-2 inhibitor versus control and addressing their effect on PWV. We set as primary efficacy outcome the change in PWV with SGLT-2 inhibitors versus placebo or control. Results: We pooled data from six trials in a total of 452 enrolled participants assigned either to SGLT-2 inhibitor or control. Overall, SGLT-2 inhibitor treatment compared to control resulted in a nonsignificant decrease in PWV. Exclusion of a trial utilizing cardiac magnetic resonance imaging for the assessment of PWV demonstrated that SGLT-2 inhibitors induce a significant reduction in PWV by 0.21 m/s. When we restricted our analysis to RCTs enrolling subjects with T2DM, we observed that SGLT-2 inhibitor compared to control resulted in a significant decrease in PWV by 0.17 m/s. Conclusion: SGLT-2 inhibitors do not decrease PWV in patients with established cardiovascular disease or cardiovascular risk factors. However, we have shown that SGLT-2 inhibitors lead to a slight, but significant decrease in PWV in patients with T2DM. The latter finding is of great value, based on the significant correlation between PWV and micro- and macro-vascular complications of T2DM.
Hybrid Approach of a Complex Aneurysm of the Subclavian-Axillary-Brachial Axis: A Case Report
Authors: Mendes Daniel , Vaz Carolina , Machado Rui , Almeida Rui 
Published: June 2022

Background Upper extremity arterial aneurysms are a rare, potentially limb-threatening disorder. Due to its rarity, the best treatment modality and outcomes are not entirely established; however, there is a consensus that open surgery is the first-line treatment. We present a complex case of an arterial aneurysm of the subclavian-axillary-brachial axis adequately treated using a hybrid surgical and endovascular treatment. Case Presentation A 59-year-old man presented to the emergency department with acute ischemia of the right upper limb. An extensive thrombosed aneurysm of the subclavian-axillary-brachial axis was identified. The treatment was carried out in two stages. In the first phase, urgent limb revascularization was performed using the great saphenous vein to perform a subclavian-brachial artery bypass. Later, the aneurysm was excluded using a vascular plug and multiple coils. Successful treatment was achieved with no morbidity for the patient. Conclusions Although the gold standard for treating upper limb aneurysms is open surgery, endovascular techniques can be a significant adjunct, reducing treatment morbidity and even mortality.