bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of alkyl substitution on the oxidative metabolism and mutagenicity of phenanthrene
Authors: Wang Danlei , Schramm Viktoria , Pool Jeroen , Pardali Eleni , Brandenburg Annemarijn , Rietjens Ivonne M. C. M. , Boogaard Peter J. 
Published: February 2022

AbstractAlkyl-substituted PAHs may be present in certain petroleum-derived products and in the environment and may eventually end up in consumer products, such as foodstuffs, cosmetics and pharmaceuticals. Safety concerns over possible exposure to alkylated PAHs have emerged. Bioactivation is a prerequisite for the mutagenicity and carcinogenicity of PAHs and has been extensively studied for non-substituted PAHs, while data on the bioactivation of alkyl-substituted PAHs are scarce. The present study investigated the effect of alkyl substitution on the CYP 450-mediated metabolism of phenanthrene and eight of its alkylated congeners by quantifying metabolite formation in rat and human liver microsomal incubations. Furthermore, the mutagenicity of four selected methylated phenanthrenes was compared to that of phenanthrene using the Ames test. The obtained results support the hypothesis that alkyl substitution shifts the oxidative metabolism from the aromatic ring to the alkyl side chain. Increasing the length of the alkyl chain reduced overall metabolism with metabolic conversion for 1-n-dodecyl-phenanthrene (C12) being negligible. 1- and 9-methyl-phenanthrene, in which the methyl group generates an additional bay region-like structural motif, showed mutagenicity toward Salmonella typhimurium TA98 and TA 100, whereas phenanthrene and also 2- and 3-methyl-phenanthrene, without such an additional bay region-like structural motif, tested negative. It is concluded that the position of the alkylation affects the metabolism and resulting mutagenicity of phenanthrene with the mutagenicity increasing in cases where the alkyl substituent creates an additional bay region-like structural motif, in spite of the extra possibilities for side chain oxidation.
On the maximal operator of a general Ornstein–Uhlenbeck semigroup
Authors: Casarino Valentina , Ciatti Paolo , Sjögren Peter 
Published: February 2022

AbstractIf Q is a real, symmetric and positive definite $$n\times n$$

n
×
n

matrix, and B a real $$n\times n$$

n
×
n

matrix whose eigenvalues have negative real parts, we consider the Ornstein–Uhlenbeck semigroup on $$\mathbb {R}^n$$

R

n

with covariance Q and drift matrix B. Our main result says that the associated maximal operator is of weak type (1, 1) with respect to the invariant measure. The proof has a geometric gist and hinges on the “forbidden zones method” previously introduced by the third author.
The influences of target size and recent experience on the vigour of adjustments to ongoing movements
Authors: Brenner Eli , Hardon Hidde , Moesman Ryan , Crowe Emily M. , Smeets Jeroen B. J. 
Published: February 2022

AbstractPeople adjust their on-going movements to changes in the environment. It takes about 100 ms to respond to an abrupt change in a target’s position. Does the vigour of such responses depend on the extent to which responding is beneficial? We asked participants to tap on targets that jumped laterally once their finger started to move. In separate blocks of trials the target either remained at the new position so that it was beneficial to respond to the jump, or jumped back almost immediately so that it was disadvantageous to do so. We also varied the target’s size, because a smaller, less vigorous adjustment is enough to place the finger within a larger target. There was a systematic relationship between the vigour of the response and the remaining time until the tap: the shorter the remaining time the more vigorous the response. This relationship did not depend on the target’s size or whether or not the target jumped back. It was already known that the vigour of responses to target jumps depends on the magnitude of the jump and on the time available for adjusting the movement to that jump. We show that the vigour of the response is precisely tuned to the time available for making the required adjustment irrespective of whether responding in this manner is beneficial.
Orientation-preserving and orientation-reversing mappings: a new description
Authors: Higgins Peter M. , Vernitski Alexei 
Published: February 2022

AbstractWe characterise the respective semigroups of mappings that preserve, or that preserve or reverse orientation of a finite cycle, in terms of their actions on oriented triples and oriented quadruples. This leads to a proof that the latter semigroup coincides with the semigroup of all mappings that preserve intersections of chords on the corresponding circle.
Cellular and soluble immune checkpoint signaling forms PD-L1 and PD-1 in renal tumor tissue and in blood
Authors: Keber Corinna U. , Derigs Marcus , Schultz Carolin , Wegner Moritz , Lingelbach Susanne , Wischmann Viktoria , Hofmann Rainer , Denkert Carsten , Hegele Axel , Hänze Jörg 
Published: February 2022

AbstractImmune checkpoint blockade therapy is a treatment option of various metastatic cancer diseases including renal cell carcinoma (RCC). Approved antibody drugs target the co-inhibitory signaling of Programmed Cell Death Ligand-1 (PD-L1) and its receptor Programmed Cell Death-1 (PD-1). The combined evaluation of PD-L1 and PD-1 at the mRNA and protein levels in tumor tissue with differentiation of tumor and immune cells as well as of soluble forms (sPD-L1) and (sPD-1) in blood is of basic interest in assessing biomarker surrogates. Here, we demonstrate that PD-L1 determined as fraction of stained tumor cells (TPS-score) correlates with PD-L1-mRNA in tumor tissue, reflecting the predominant expression of PD-L1 in tumor cells. Conversely, PD-1 in immune cells of tumor tissue (IC-score) correlated with PD-1-mRNA tissue levels reflecting the typical PD-1 expression in immune cells. Of note, sPD-L1 in blood did not correlate with either the TPS-score of PD-L1 or with PD-L1-mRNA in tumor tissue. sPD-L1 released into the supernatant of cultured RCC cells closely followed the cellular PD-L1 expression as tested by interferon γ (IFNG) induction and siRNA knockdown of PD-L1. Further analysis in patients revealed that sPD-L1 significantly increased in blood following renal tumor resection. In addition, sPD-L1 correlated significantly with inflammation marker C-reactive protein (CRP) and with PD-L1 mRNA level in whole blood. These results indicate that the major source of sPD-L1 in blood may be peripheral blood cells and not primarily tumor tissue PD-L1.
Approaches to Surgical Debridement in Necrotizing Soft Tissue Infections: Outcomes of an Animated, Interactive Survey
Authors: Suijker Jaco , Hofmans Fabienne A. C. , van Zuijlen Paul P. M. , Cense Huib A. , Bonjer H. Jaap , Vries Annebeth Meij‐de 
Published: February 2022

AbstractBackgroundNecrotizing soft tissue infections (NSTI) affect long‐term quality of life in survivors. Different approaches to debridement may influence quality of life. The aim of this study was to assess the current practice of the debridement of NSTI in the Netherlands.MethodsAn animated, interactive online survey was distributed among general surgeons and plastic surgeons in the Netherlands. Two NSTI‐cases were presented, followed by questions regarding the preferred surgical approach. Case one described a woman with a swollen, red leg, with signs of sepsis and without visible necrosis. Case two described an immunocompromised man with septic shock syndrome and extensive necrosis.ResultsIn total 232 responses were included (143 general surgeons, 89 plastic surgeons). In case one, 32% chose to preserve all skin, while 17% chose to resect all skin above the affected fascia, including normal‐looking skin. In case two, all participants resected necrotic skin, and most (88%) also blue discolored skin. While 32% did not resect more than blue discolored and necrotic skin, 35% also resected red‐colored skin, and 21% all skin overlying the affected fascia, including normal colored skin. Respondents working in a hospital with a burn center tended to preserve more skin, whereas plastic surgeons chose more often for skin resection compared to general surgeons.ConclusionsBy using a novel approach to a survey, the authors demonstrate the existence of extensive practice variety regarding the approach to debridement of NSTI among Dutch general and plastic surgeons. Consensus is needed, followed by targeted education of surgeons.
Exact prediction intervals for future exponential and Pareto lifetimes based on ordered ranked set sampling of non-random and random size
Authors: Barakat H. M. , Newer Haidy A. 
Published: February 2022

AbstractIn the present paper, two pivotal statistics are suggested to construct prediction intervals of future observations from the exponential and Pareto distributions in the context of ordered ranked set sample. Our study encompasses two cases. The first case, when the sample size is assumed to be fixed and the second case when the sample size is assumed to be a positive integer-valued random variable. In addition to deriving explicit forms for the distribution functions of the two pivotal statistics, we consider some special cases for the random size of the sample. Moreover, a simulation study is carried out to assess the efficiency of the suggested methods. Finally, an example representing lifetime data is analyzed.
Effect of orographic gravity wave drag on Northern Hemisphere climate in transient simulations of the last deglaciation
Authors: Snoll Brooke , Ivanovic Ruza F. , Valdes Paul J. , Maycock Amanda C. , Gregoire Lauren J. 
Published: February 2022

AbstractLong transient simulations of the last deglaciation are increasingly being performed to identify the drivers of multiple rapid Earth system changes that occurred in this period. Such simulations frequently prescribe temporal variations in ice sheet properties, which can play an important role in controlling atmospheric and surface climate. To preserve a model’s standard performance in simulating climate, it is common to apply time dependent orographic variations, including parameterised sub-grid scale orographic variances, as anomalies from the pre-industrial state. This study investigates the causes of two abrupt climate change events in the Northern Hemisphere extratropics occurring between 16 and 14 thousand years ago in transient simulations of the last deglaciation from the Hadley Centre coupled general circulation model (HadCM3). One event is characterized by regional Northern Hemisphere changes comprising a centennial scale cooling of ~ 10 °C across Fennoscandia followed by rapid warming in less than 50 years as well as synchronous shifts in the Northern Annular Mode. The second event has comparable but temporally reversed characteristics. Sensitivity experiments reveal the climate anomalies are exclusively caused by artificially large values of orographic gravity wave drag, resulting from the combined use of the orographic anomaly method along with a unique inclusion of transient orography that linearly interpolates between timesteps in the ice sheet reconstruction. Palaeoclimate modelling groups should therefore carefully check the effects of their sub-grid scale orographic terms in transient palaeoclimate simulations with prescribed topographic evolution.
The role of serum D-Dimer for the diagnosis of periprosthetic shoulder infection
Authors: Akgün Doruk , Al-Muhtaresh Faisal , Paksoy Alp , Lacheta Lucca , Minkus Marvin , Karczewski Daniel , Moroder Philipp 
Published: February 2022

Abstract
Introduction
D-Dimer was recently identified as an additional biomarker in the diagnosis of hip and knee periprosthetic joint infection (PJI). Currently, there is only one study in literature dealing with the role of D-Dimer in the diagnosis of shoulder PJI. The purpose of this study was, therefore, to validate the sensitivity and specificity of D-Dimer in detecting shoulder PJI.

Materials and methods
All patients, who underwent septic or aseptic revision shoulder arthroplasty in our institution between November 2018 und March 2021, were analyzed. Our cohort consisted of 30 patients, of that 14 (47%) had a shoulder PJI according the last proposed criteria of the International Consensus Meeting. The diagnostic validity of serum D-Dimer regarding the detection of PJI was analyzed.

Results
The mean D-Dimer level was significantly higher for the patients with shoulder PJI compared to patients with aseptic failure (1.44 ± 1 mg/l vs. 0.76 ± 0.6 mg/l, p = 0.025). Coagulase-negative staphylococci were the most commonly isolated pathogens, in 9/14 patients (64%), followed by Cutibacterium acnes in 5/14 patients (36%). According to the ROC analysis, a serum D-Dimer threshold of 0.75 mg/l had a sensitivity of 86% and a specificity of 56% for detection of a shoulder PJI. The area under curve was 0.74. A serum C-reactive protein (CRP) cutoff of 10 mg/l showed a sensitivity of 69% and a specificity of 88%. When both serum D-Dimer and CRP above the thresholds of 0.75 mg/l and 10 mg/l, respectively, were used to identify a PJI the sensitivity and specificity were 57% and 100%, respectively.

Conclusions
Serum D-Dimer showed a good sensitivity but a poor specificity for the diagnosis of shoulder PJI. Combination D-Dimer and CRP led to improvement of the specificity, however, at the cost of sensitivity. Thus, combination of both methods may be used as a confirmatory test in the diagnosis of shoulder PJI but not to rule out infection.

Level of evidence
Diagnostic level II.
Evaluation of the Bonebridge BCI 602 active bone conductive implant in adults: efficacy and stability of audiological, surgical, and functional outcomes
Authors: Cywka Katarzyna B. , Skarzynski Piotr H. , Krol Bartlomiej , Hatzopoulos Stavros , Skarzynski Henryk 
Published: February 2022

Abstract
Purpose
(1) To assess the effectiveness and safety of a bone-conduction implant, the Bonebridge BCI 602, in adults with conductive or mixed hearing loss. (2) To investigate whether the Bonebridge BCI 602 is at least as effective as the Bonebridge BCI 601 in such patients.

Methods
The study group included 42 adults who had either conductive or mixed hearing loss. All patients underwent Bonebridge BCI 602 implant surgery. Before and after implantation, pure-tone audiometry, speech recognition tests (in quiet and noise), and free-field audiometry were performed. Word recognition scores were evaluated using the Polish Monosyllabic Word Test. Speech reception thresholds in noise were assessed using the Polish Sentence Matrix Test. Subjective assessment of benefits was done using the APHAB (Abbreviated Profile of Hearing Aid Benefit) questionnaire.

Results
The APHAB questionnaire showed that difficulties in hearing decreased after BCI 602 implantation. Both word recognition in quiet and speech reception threshold in noise were significantly better after BCI 602 implantation and remained stable for at least 12 months. A significant advantage of the device is a reduced time for surgery while maintaining safety. In this study, the mean time for BCI 602 implantation was 28.3 min ± 9.4.

Conclusions
The second-generation Bonebridge BCI 602 implant is an effective hearing rehabilitation device for patients with conductive or mixed hearing loss. Patient satisfaction and audiological results confirm its efficacy and safety. Its new shape and dimensions allow it to be used in patients previously excluded due to insufficient or difficult anatomical conditions. The new BCI 602 implant is as effective as its predecessor, the BCI 601.
Body-kun/body-chan style model figures for artists in forensic visualization applications
Authors: Potente Stefan , Heinbuch Sara , Ramsthaler Frank , Schmidt Peter 
Published: February 2022

AbstractPosture and body position are often in the focus of forensic medicine. Visualization for the purposes of documentation, teaching, scientific presentation or expert opinion in court is often desired. Plenty of possible tools to support visualization are available. However, there is a significant gap between quick drawings and more complex techniques. Body-chan (female) and body-kun (male) artist’s model figurines (genericized trademark) may provide a useful means to fill this gap. These models, about 12–15 cm in height, are multi-articulated humanoids of realistic proportions, intended to serve as models for manga (japanese comic) drawing. Plenty of different models are available in different quality which usually are equipped with interchangeable hand and feet attachments, a frame for ‘levitating’ positions as well as assorted objects to scale. These inexpensive models may be positioned quickly and intuitively. Photodocumentation from various angles can be performed using a mobile phone camera. Images may be further improved applying digital image manipulation software. Taken together, the process is quick and intuitive and the level of achievable complexity is sufficient for many forensic applications.
Coronary angiography in acute ischemic stroke patients: frequency and determinants of pathological findings in a multicenter cohort study
Authors: Litmeier Simon , Meinel Thomas R. , von Rennenberg Regina , Kniepert Joachim U. , Audebert Heinrich J. , Endres Matthias , Jung Simon , Scheitz Jan F. , Nolte Christian H. 
Published: February 2022

Abstract
Background
Myocardial injury as indicated by cardiac troponin elevation is associated with poor prognosis in acute stroke patients. Coronary angiography (CAG) is the diagnostic gold-standard to rule-out underlying obstructive coronary artery disease (CAD) in these patients. However, weighing risks and benefits of coronary angiography (CAG) against each other is particularly challenging, because stroke patients undergoing CAG may have a higher risk for secondary intracranial bleeding. Current guidelines remain vague. Thus, the aim of this study was to analyze frequency of pathological findings of CAG and associated clinical factors.

Methods
We analyzed indications and frequency of CAG performed in acute ischemic stroke patients in clinical routine in two European tertiary care hospitals from 2011 to 2018. All data were obtained retrospectively. Multiple logistic regression analyses were performed to identify variables associated with absence of obstructive coronary artery disease defined as presence of at least one coronary vessel stenosis ≥ 50%.

Results
A total of 139 AIS patients underwent CAG. Frequent indications for CAG were suspected acute coronary syndrome (N = 114) or scheduled cardiac surgery (N = 25). Acute coronary stenting was applied in 51/139 patients. Among patients with suspected acute coronary syndrome, no obstructive CAD was found in 27/114 patients. Absence of obstructive CAD was associated with insular cortex lesions, no clinical symptoms for ACS, less than three cardiovascular risk factors, younger age and normal wall motion.

Conclusion
Several variables suggest absence of CAD in AIS patients and may help in clinical decision making in stroke patients with myocardial injury.
The time course of different neuromuscular adaptations to short-term downhill running training and their specific relationships with strength gains
Authors: Bontemps Bastien , Gruet Mathieu , Louis Julien , Owens Daniel J. , Miríc Stella , Erskine Robert M. , Vercruyssen Fabrice 
Published: February 2022

Abstract
Purpose
Due to its eccentric nature, downhill running (DR) training has been suggested to promote strength gains through neuromuscular adaptations. However, it is unknown whether short-term chronic DR can elicit such adaptations.

Methods
Twelve untrained, young, healthy adults (5 women, 7 men) took part in 4 weeks’ DR, comprising 10 sessions, with running speed equivalent to 60–65% maximal oxygen uptake ($$\dot{V}$$

V
˙

O2max, assessed at weeks 0 and 4). Isometric and isokinetic knee-extensor maximal voluntary torque (MVT), vastus lateralis (VL) muscle morphology/architecture (anatomical cross-sectional area, ACSA; physiological CSA, PCSA; volume; fascicle length, Lf; pennation angle, PA) and neuromuscular activation (VL EMG) were assessed at weeks 0, 2 and 4.

Results
MVT increased by 9.7–15.2% after 4 weeks (p < 0.01). VL EMG during isometric MVT increased by 35.6 ± 46.1% after 4 weeks (p < 0.05) and correlated with changes in isometric MVT after 2 weeks (r = 0.86, p = 0.001). VL ACSA (+2.9 ± 2.7% and +7.1 ± 3.5%) and volume (+2.5 ± 2.5% and +6.6 ± 3.2%) increased after 2 and 4 weeks, respectively (p < 0.05). PCSA (+3.8 ± 3.3%), PA (+5.8 ± 3.8%) and Lf (+2.7 ± 2.2%) increased after 4 weeks (p < 0.01). Changes in VL volume (r = 0.67, p = 0.03) and PCSA (r = 0.71, p = 0.01) correlated with changes in concentric MVT from 2 to 4 weeks. $$\dot{V}$$

V
˙

O2max (49.4 ± 6.2 vs. 49.7 ± 6.3 mL·kg−1·min−1) did not change after 4 weeks (p = 0.73).

Conclusion
Just 4 weeks’ moderate-intensity DR promoted neuromuscular adaptations in young, healthy adults, typically observed after high-intensity eccentric resistance training. Neural adaptations appeared to contribute to most of the strength gains at 2 and 4 weeks, while muscle hypertrophy seemed to contribute to MVT changes from 2 to 4 weeks only.
Surgical and endovascular revascularization of chronic mesenteric ischemia
Authors: Wolk Steffen , Kapalla Marvin , Ludwig Stefan , Radosa Christoph , Hoffmann Ralf-Thorsten , Weitz Jürgen , Reeps Christian 
Published: February 2022

Abstract
Purpose
Chronic mesenteric ischemia (CMI) is a rare but life-threatening disease. This study reviewed outcomes in patients treated surgically for CMI by open treatment (OT) and endovascular treatment (ET), analyzing risk factors for endovascular failure.

Methods
Clinical data for 36 patients treated for CMI from 2007 to 2017 were retrospectively analyzed. The study’s primary endpoint was symptom-free survival. The secondary endpoint was the primary technical success for endovascular and open surgical treatments. Risk factors for endovascular failure were identified by using univariate analysis.

Results
Patients were analyzed as treated: 21 patients (58.3%) in the ET and 15 (41.6%) in the OT group. Overall, 20 patients (56%) presented with abdominal angina, 9 (25%) with rest pain, and 7 (19%) without symptoms. An ET was initially attempted in 31 patients (86.1%). The conversion rate from ET to OT was 32.3%, which resulted in a primary technical success of 67.6% in ET and 100% in OT. Six patients from the ET group (19.3%) required surgical revision due to restenosis. One-year (OT 91.6% vs. ET 96.8%; n.s.) and three-year primary patency (OT 91.6% vs. ET 80.6%; n.s.) as well as 3-year symptom-free survival did not differ between the groups (OT 62.5% vs. ET 69.4%; n.s). Overall, in-hospital mortality was 2.8% (n = 1), which was not statistically different between the groups (OT 6% vs. ET 0%; n.s.). High-grade stenosis of the superior mesenteric artery tended to be associated with higher technical failure (P = 0.06).

Conclusions
ET showed a comparable perioperative outcome with higher technical failure. OT was distinguished by excellent early and late technical success.