bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative Analysis to Identify Diagnostic Biomarkers (HSP90AA1, VCAM1 and MX1) and Reveal Immune Cell Infiltration Changes in Patients with Vitiligo
Authors: Huang Youming , Xu Danfeng , Fan Yibin , Ding Xiaoxia 
Published: March 2022

Abstract
Background
This current study applied bioinformatics analysis to reveal the potential role of immune-related genes and blood immune cell infiltration changes in vitiligo development.
Methods
The gene expression level was obtained from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were identified between blood samples from patients with segmental vitiligo (SV), non-segmental vitiligo (NSV) and healthy controls (CL), and the DEGS from two sets were used for further analysis respectively, including hub gene exploration, enrichment analysis, interaction network construction and engaged pathway analysis. Immune-related gene crosstalk analysis, gene-miRNA analysis, and AUC analysis were performed for further gene screening. Furthermore, immune cell infiltration analysis was also performed to reveal the change of systemic environment in patients with vitiligo.
Results
There were 161 DEGs in the comparison of SV and CL, 11 DEGs in NSV and CL. Functional enrichment analysis indicated that DEGS in NSV group mainly involved in responses to virus and type 1 interferon signaling pathway, while DEGS in SV group mainly involved in IL-17 signaling pathway and TNF signaling pathway. After PPI network construction, immune-related gene crosstalk analysis, and verification of diagnostic markers, totally 4 genes were obtained as diagnostic markers (VCAM1, HSP90AA1, TNF from SV group and MX1 from NSV group). Moreover, we found that the infiltrated percentage of resting memory CD4 T cells were both higher in blood samples from patients with segmental vitiligo and non-segmental vitiligo.
Conclusion
For the first time, VCAM1, HSP90AA1, and MX1 were found associated with vitiligo and had good diagnostic significance. Meanwhile, we found that vitiligo patients had more proportion of resting memory CD4 T cells infiltration in blood. These results may provide new research ideas for the pathogenesis and diagnosis of vitiligo.
The association between coagulation/fibrinolysis function and serum lipid levels in people with advanced knee osteoarthritis: a cross-sectional study
Authors: Teng Ruo-Ling , Zhao Jie , Zheng Xiao-Ling , Sun Han 
Published: March 2022

Abstract
Background. The aim of this study was to explore the relationship between coagulation/fibrinolysis function and serum lipid levels in people with advanced knee osteoarthritis (KOA).Methods. From January 1st 2013 to January 1st 2018, a total of 206 patients underwent total or unicompartmental knee arthroplasty in Changzhou No.1 People’s Hospital were enrolled consecutively in this study. Baseline characteristics and preoperative laboratory indicators of patients were collected. The association between coagulation/fibrinolysis function and serum lipid levels was analyzed using Spearman correlation analysis and multiple linear regression.Results. Spearman correlation analysis revealed that coagulation/fibrinolysis function was associated with serum lipid levels, especially prothrombin time was moderately negatively correlated with Apolipoprotein-A1 (r=-0.4585, P<0.05). The multiple linear regression analyses were performed with serum lipid levels as the independent variables and coagulation/fibrinolysis function parameters as the dependent variables after adjusting for sex, age, body mass index, hypertension, diabetes mellitus and coronary heart disease. We found that the regression analysis was reliable (its imitation degree was ideal, adjusted R^2 = 0.3108) and predictive (P<0.0001) when the dependent variable was prothrombin time. The significant contributors to prothrombin time were triglyceride (P<0.001), high density lipoprotein cholesterol (P=0.003), Apolipoprotein-A1 (P=0.017) and Lipoprotein (a) (P=0.015).Conclusions. The results of this study showed that coagulation/fibrinolysis function was correlated with serum lipid levels in patients with advanced KOA, although the correlation may be small. It may be hypothesized that strategies for lipid control may help improve coagulation/fibrinolytic dysfunction in patients with KOA.Trial registration. This clinical study was retrospectively registered in a publicly available registry because this study was a retrospective study began in 2018. This study was approved by the Ethics Committee of the Third Affiliated Hospital of Soochow University in Jiangsu Province of China (No.CZYY2021187).
“Immune Hemolytic Anemia in Patients with Lymphoid Malignancy in Tanzania”
Authors: Uwizeyimana Gilbert , Ally Mwashungi , Yonazi Mbonea , Chamba Clara , Nasser Ahlam , Luzzatto Lucio 
Published: March 2022

Abstract
Background
Hematological malignancies of the lymphoid lineage such as multiple myeloma, lymphomas and lymphoid leukemia are a burden to the world especially in Tanzania; anaemia is one of the presenting symptoms of these conditions and is found in almost 100% of the patients at diagnosis. Of particular importance Immune Hemolytic Anemia (IHA) which has a poor response to treatment and subsequently poor prognosis when it is associated with lymphoid malignancies. The aim of this study is to determine the magnitude of immune hemolytic anemia in lymphoid malignancies in Tanzania
Methods
This was a cross sectional study that recruited 86 patients with lymphoid malignancies attending both Ocean Road Cancer Institute (ORCI) and Muhimbili National Hospital (MNH). The clinical features were captured using a well-structured questionnaire. Laboratory results for Full Blood Count (FBC), reticulocytes count, total, indirect and direct bilirubin and Lactate Dehydrogenase (LDH) were retrieved from the laboratory reporting system. Additionally, a blood sample for the Direct Antiglobulin Test (DAT) was taken from all participants. This test was performed at the Central Pathology Laboratory (CPL) of MNH. Data analysis was performed using Statistical Package for the Social Sciences (SPSS 20.0) and Microsoft excel 2010. Data analysis was performed using South Texas Art Therapy Association (STATA 15.0).
Results
IHA, indicated by a positive DAT, was found in 10% of patients with lymphoid malignancies. Analysis of DAT among patients never exposed to chemotherapy showed a prevalence of 22%. Among 9 IHA cases, 2 were CLL (Chronic Lymphoid Leukaemia), 2 were MM (Multiple Myeloma), 3 was NHL (Non-Hodgkin Lymphoma), 1 was HL (Hodgkin Lymphoma) and 1 SLL (Small Lymphocytic Lymphoma). All 9 cases had decompensated hemolytic anemia.
Conclusion
IHA is a real complication of chronic lymphoid malignancies and DAT should be included in tests done during the course of management.
Bone Metastasis From Thyroid Papillary Carcinoma Detected on PET/CT: 68Ga-FAPI Better Than 18F-FDG?
Authors: Chen Jie , Li Chongjiao , Tian Yueli , Wen Bing , He Yong 
Published: March 2022

Abstract
In the present study, we here report a case of 71-year-old woman, with bone destruction of L5 vertebral body showing lower of SUVmax for FAPI compared to FDG PET/CT imaging. To our knowledge, this present case is the first study to reveal that 68Ga-FAPI had lower tumour-to-background ratio in detecting bone metastasis from PTCMCa-FV.
The “Vestibular Eye Sign” – “VES”.A new radiological sign of vestibular neuronitis can help to determine the affected vestibule and support the diagnosis.
Authors: Farhat Raed , Awad Anan Abu , Shaheen Waleed Abu , Alwily Diaa , Avraham Yaniv , Najjar Razi , Merchavy Shlomo , Massoud Saqr 
Published: March 2022

Abstract
Introduction: Nystagmus is a valuable clinical finding. Although nystagmus is often described by the direction of its quick phases, it is the slow phase that reflects the underlying disorder. Vestibular neuritis (VN) is thought to be the result of inflammation of the vestibular portion of the eighth cranial nerve. The clinician's task is to differentiate this benign self-limiting disease from other central causes, such as cerebrovascular syndromes. The aim of our study was to describe a new radiological diagnostic sign called “Vestibular Eye Sign”-VES. This sign is defined as an eye deviation that correlates with the slow phase of nystagmus (vestibule pathological side), which is seen in acute vestibular neuronitis and can be assessed on a CT head scan. Materials and methods: A total of 1250 patients were diagnosed with vertigo in the Emergency Department at Ziv Medical Center (ED) in Safed, Israel. The data of 315 patients who arrived at the ED between January 2010 and January 2022 were collected, with criteria eligible for the study. Patients were divided into 4 groups: Group A, “pure VN”, Group B, “non-VN aetiology”, Group C, BPPV patients, and Group D, patients who had a diagnosis of vertigo with unknown aetiology. All groups underwent head CT examination while in the ED.Results: In Group A, pure vestibular neuritis was diagnosed in 70 (22.2%) patients. Regarding accuracy, VES (Vestibular Eye Sign) was found in 65 patients in group A and 8 patients in group B and had a sensitivity of 89%, specificity of 75% and a negative predictive value of 99.4% in group A – pure vestibular neuronitis.Conclusion – VN is still a clinical diagnosis, but if the patient undergoes head CT, we suggest using the “vestibular eye sign” as a complementary sign. As per our findings, this is a valuable sign on CT imaging for diagnosing the pathological side of isolated pure VN. It is sensitive to support a diagnosis with a high negative predictive value.
Low-expression of miR-1929-3p mediates murine cytomegalovirus-induced fibrosis in cardiac fibroblasts via targeting endothelin A receptor/NLRP3 inflammasome pathway
Authors: He Fang , Li Jiayi , Wang Yongjia , Qu Yuanyuan , Zhou Wei , Zhong Hua , Wang LaMei , Xi DongMei , Tang Na 
Published: March 2022

Abstract
It has shown that mouse cytomegalovirus (MCMV) infection induced low-expression of miR-1929-3p is closely related to hypertensive myocardial remodeling in our previous research. In this study, we further explored the molecular mechanism of myocardial remodeling induced by miR-1929-3p after MCMV infection on cellular level. We built MCMV infected mouse cardiac fibroblasts (MMCFs) as main cell model. First, MCMV infection caused low-expression of miR-1929-3p and higher mRNA and protein expression of its target gene endothelin receptor type A (ETAR) in mouse cardiac fibroblasts (MCFs), which showed an internal relation to myocardial fibrosis (MF) on high proliferation, phenotypic transformation (α-SMA) and collagen expression in MMCFs. Then, transfection of miR-1929-3p mimic down-regulated the high expression of ETAR and relieve these adverse effects in MMCFs. Inversely, these effects were exacerbated by miR-1929-3p inhibitor. Second, transfection of endothelin receptor type A over-expressed adenovirus (adETAR) reversed these positive effects of miR-1929-3p mimic about MF improvement. Third, it presented strong inflammatory response in MMCFs with more expression of NOD like receptors pyrin domain containing 3 (NLRP3) and secretion of interleukin-18 (IL-18). However, we found ETAR antagonist BQ123 and selected NLRP3 inflammasome inhibitor MCC950 effectively eliminated the inflammatory response induced by both MCMV infection and miR-1929-3p inhibitor. Moreover, the supernatant of MCFs was shown to be related to cardiomyocyte hypertrophy. Our research revealed that MCMV infection promotes MF by inducing down-regulation of miR-1929-3p and high expression of ETAR which activate NLRP3 inflammasome in MCFs.
Trend of Seasonal and Annual Rainfall in Semi-arid Districts of Karnataka, India: Application of Innovative Trend Analysis Approach
Authors: Chowdari KK , Barma Surajit Deb , Bhat Nagaraj , Girisha R , Gouda K.C. , Mahesha Amai 
Published: March 2022

Abstract
Trend analysis of rainfall is often carried out in water resources management to understand its distribution over a given region. The cumulative seasonal and annual rainfall derived from monthly datasets spanning 102 years (1901–2002) for 11 districts of the semi-arid Karnataka, India, was used for the trend analysis. The two-step homogeneous test approach was carried out on all the time series. Then, lag-1 autocorrelation was conducted only on homogeneous time series. Only 78.18% of the total time series data was detected as homogeneous, and 95.35% of time series data were found to have insignificant autocorrelation. Then, the Innovative Trend Analysis (ITA) method was applied to 43 homogeneous rainfall time series, MK and SR tests to 41 time series, and mMK test to two time series. The MK and SR tests detected 14.63% of the time series as a significant trend, whereas the ITA method could detect 93.02% of the total time series data. The MK and SR tests captured significant trends for the winter season in two districts, but the two tests detected a significant trend only in one district for the summer season. None could be captured for monsoon season. A significant trend was captured for the post-monsoon season in two districts; however, the tests could detect a significant trend in one district for annual rainfall. The mMK test revealed a positive trend for the post-monsoon season in a district. The ITA method could capture a significant trend for all the seasons in most districts.
Association between extra-spousal partnerships and HIV prevalence among married individuals in Rakai, Uganda
Authors: TIGAIZA ARNOLD , MATOVU JOSEPH KB 
Published: March 2022

Abstract
Introduction: The association between extra-spousal partnerships and HIV in married or cohabiting individuals remains controversial. We established the association between extra-spousal partnerships and HIV prevalence among married or cohabiting individuals in Rakai, Uganda.Methods: This secondary analysis uses data from a large cross-sectional study conducted among 2,135 married or cohabiting individuals aged 15-49 years in Rakai, Uganda, between November 2013 and February 2014. Data were collected on socio-demographic and behavioral characteristics, including extra-spousal partnerships. We used a modified Poisson regression model to assess the association between extra-spousal partnerships and HIV prevalence among married or cohabiting individuals living in three study communities with differing HIV prevalence. Stata version 14.0 was used for data analysis. Results: Of 2,103 respondents included in the analysis, 13.4% (282/2103) reported extra-spousal partnerships; 4.5% (n=49) among females and 22.9% (n=233) among males. Of those reporting extra-spousal partnerships, 48.6% (n=137) were in their second or higher order marriage while 74.1% (n=209) reported a marital duration of six or more years. HIV prevalence was higher among those reporting extra-spousal partnerships (14.6%, 36/247) than in those who did not (7.6%, 120/1575; P<0.0001). In multivariable analyses, we found that engaging in extra-spousal relationships increased the risk of HIV infection by 67% (adjusted Prevalence Ratio [adj. PR] = 1.67; 95%CI: 1.16, 2.39), after adjusting for potential and suspected confounders. Conclusion: We found that engaging in extra-spousal partnerships increased the risk of HIV infection by more than two-thirds. These findings suggest a need for targeted HIV prevention interventions for married or cohabiting individuals who report extra-spousal partnerships.
Favorable Safety Profile Of Moderate Hypofractionated Over Normofractionated Radiotherapy In Breast Cancer Patients: A Multicentric Prospective Real-Life Data Farming Analysis
Authors: Issoufaly Irfane , Petit Claire , Guihard Sébastien , Eugène Rémi , Jung Loic , Clavier Jean Baptiste , Vernat Stéphanie Servagi , Bellefquih Sara , Verret Benjamin , Bonnet Naima , Deutsch Eric , Rivera Sofia 
Published: March 2022

Abstract
Background: Moderately hypofractionated whole-breast radiotherapy (HFRT) has proven to be as safe and efficient as normofractionated radiotherapy (NFRT) in randomized trials resulting in major changes in clinical practice. Toxicity rates observed in selected clinical trial patients may differ from those observed in unselected patients with possible comorbidities and frailty in real-life. This study aimed to examine the influence of HFRT versus NFRT on acute toxicity and identify risks factors of dermatitis in real-life patients.Materials and Methods: Prospective data from breast cancer patients, treated with locoregional radiotherapy between November 2015 and February 2020 in 3 comprehensive cancer centers, were collected. Through a systematic data-farming strategy, acute toxicity evaluation forms (CTCAEv4.0) were prospectively completed and extracted electronically. The results from each center were then anonymously merged into a single database for analysis. A Chi-2 test was used to compare HFRT and NFRT. Furthermore, risk factors of dermatitis were identified in a sub-study (622 patients) by multivariate logistic regression analysis.Results: In total, 3518 T0-4 N0-3 mostly M0 (85.8%) breast cancer patients with a median age of 60.7 (24–96 years old) were analyzed. Acute grade 2-3 dermatitis, grade 1-3 breast oedema, and grade 1-2 hyperpigmentation were less frequent with HFRT versus NFRT: respectively 8.9% vs 35.1% (Chi-2=373.7; p<0.001), 29.0% vs 37.0% (Chi-2=23.1; p<0.001) and 27.0% vs 55.8% (Chi-2=279.2; p<0.001). Fewer patients experienced pain with HFRT versus NFRT: 33.4% vs 53.7% respectively (Chi-2=137.1; p<0.001). High BMI (OR=2.30 [95% CI, 1.28-4.26], p<0.01), large breast size (OR=1.88 [95% CI, 1.07-3.28], p<0.01) and lumpectomy over mastectomy (OR=0.52 [95% CI, 0.27-0.97], p<0.05) were associated with greater risk factors of grade 2-3 dermatitis in multivariate analysis regardless of NFRT or HFRT. Conclusion: The results of this study suggests that breast HFRT may be a better option even for patients with a high BMI or large breast size. Acute toxicity was low to mild, and lower with HFRT compared to NFRT. Results from real-life data were robust, and support the use of HFRT beyond randomized study populations. Long-term real-life data awaits further investigation.
Evolving Shallow-conduit Container Affects the Lava Fountaining during the 2021 Fagradalsfjall Eruption, Iceland
Authors: Eibl Eva P. S. , Thordarson Thor , Höskuldsson Ármann , Gudnason Egill Á. , Dietrich Thoralf , Hersir Gylfi Páll , Ágústsdóttir Thorbjörg 
Published: March 2022

Abstract
Pulsating behaviour is observed in volcanic phenomena ranging from caldera collapses to explosions, spattering or lava fountaining. The repeating processes can define irregular, regular or systematically changing patterns. These patterns yield information about the subsurface structure, which often is not considered in detail. We analyse the pattern of 7058 lava fountaining episodes that occur between 2 May and 14 June 2021 during the Fagradalsfjall eruption, Iceland. Our seismometer records the lava fountaining episodes as tremor pulses. We analyse the seismic tremor amplitude, the pulse duration, the repose time and the sum of pulse duration and repose time (cycle duration). We define six periods characterised by different patterns: Three periods feature long pulses that exponentially shorten with time. One period features coexisting long and short pulses in a haphazard sequence. One period shows a stable pulsing duration but increasing repose time and one period has stable, short pulses and repose times. We conclude that the episodic fountaining starts because a shallow-conduit container forms on 2 May shifting the magma degassing from sustained continuous to an episodic state. This situation evolves until 11 May when a semi-stable state is reached. The length of the repose times are most likely influenced by the amount of outgassed magma present in the uppermost part of the shallow conduit. Finally, we suggest that the vent is mechanically eroded and widens with time causing increasing seismic tremor amplitudes. However, the trends are frequently punctuated by partial crater wall collapses that temporarily disrupt the system.
How do multiple masked relevant prime stimuli affect a conscious response: Two prime arrows produce independent priming effects on the target response
Authors: Tu Shen , Liu Chengzhen , Lv Jieyu , Jou Jerwen , Wang Dingding , Song Lei , Qiu Jiang 
Published: March 2022

Abstract
Since there are a lot of sources of unconscious information in our mind, there is a possibility that multiple channels of unconscious information can affect a response at the same time. However, this question has been largely ignored by the researchers. In the present study, we presented two opposite pointing arrows as the masked primes followed by a target arrow. The results suggested that the two pointing directions of the two prime arrows influenced the response to the target simultaneously and independently, i.e. the overall priming effect caused by the two opposite pointing prime arrows was equal to the net effect of the individual congruent effect elicited by the same pointing prime arrow and the individual incongruent priming effect induced by the opposite pointing prime arrow. In addition, in Experiment 1, a biased delayed response to the target was observed when the target arrow and the opposite pointing prime arrow were closely positioned in space due to Gestalt proximity grouping.
Risperidone and Aripiprazole in Children with Autism Spectrum Disorder Substantially Improves Core Signs and Symptoms in Combination with Standard Supportive Therapies: A Large, Single-Center, Retrospective Case Series
Authors: ALSAYOUF HAMZA , Talo Haitham , Marisa  
Published: March 2022

Abstract
Background: There are a number of medications prescribed to address comorbid challenging behaviors in children with autism spectrum disorder (ASD), including risperidone and aripiprazole. This retrospective case series reports the use of these drugs in children aged 2 to 13 years.Methodology: A total of 82 children (mean age, 5 years; 79% male) with ASD treated at the Kids Neuro Clinic and Rehab Center in Dubai between January 2020 and September 2021 were included in this retrospective case series. All patients had comorbid challenging behaviors that were resistant to standard supportive therapies alone and warranted pharmacological intervention. The Childhood Autism Rating Scale – 2nd Edition Standard form (CARS2-ST) and the Clinical Global Impression (CGI) – Severity (CGI-S) and CGI – Improvement (CGI-I) scales were used to assess the severity of ASD at baseline and to monitor response to treatment with risperidone or aripiprazole. Results: Besides the expected improvement in comorbid challenging behaviors, 79/82 patients (96%) attained a CGI-I score of 2 or 1 following treatment, and 35/82 patients (43%) achieved both a CGI-I score of 1 and minimal-to-no symptoms as per the CARS2-ST test, with complete resolution of their ASD signs and symptoms. The differences in the overall mean CARS2-ST and CGI-S scores pre- and post-treatment were statistically significant (Z = −7.86, P < 0.0001 for both), with pre- and post-treatment mean values of 42 and 23 for CARS2-ST, respectively, and 6 and 2 for CGI-S, respectively. The main side effects were asymptomatic elevated prolactin (n=12) and excessive weight gain (n=2).Conclusion: When combined with standard supportive therapies, chronic treatment with antipsychotic medications, either with or without medications for attention deficit hyperactivity disorder, was well tolerated and effective in the treatment of ASD core symptoms and comorbid behaviors in young children. Double-blind, placebo-controlled clinical trials are needed to verify these findings.