bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infants in Dutch daycare: Exploring fine‐grained dimensions of temperament
Authors: Fukkink Ruben G. 
Published: July 2022

AbstractInfants attend daycare at an early age, which raises questions about children's sensitivity to the childcare environment and the role of different temperamental traits in their development in the early years. In a two‐year longitudinal study with parent‐ and caregiver‐reported data for Dutch children at the age of 1 and 2 years (120 children from 92 groups), we explored fine‐grained dimensions of negative affect and their relationship with socio‐emotional functioning. Especially shyness, frustration and soothability proved robust predictors of socio‐emotional development across parent‐ and caregiver‐reported data with both concurrent (Year‐1) and predictive associations (Year‐2). Also, the quality of caregiver–child interactions moderated caregiver‐reported child wellbeing and competence. Infants that are open to social contacts, are easy to comfort and have low levels of frustration, and have higher levels of wellbeing and less problem behaviour in early daycare than peers with higher levels of shyness and frustration and relatively low levels of soothability.
Head position and vacuum‐assisted delivery using the Kiwi Omnicup
Authors: Schreiber Hanoch , Cohen Gal , Shechter Maor Gil , Haikin Herzberger Einat , Biron‐Shental Tal , Markovitch Ofer 
Published: July 2022

AbstractObjectiveTo evaluate the association between fetal head position during prevacuum assessment and adverse outcomes.MethodThis retrospective cohort study included all vacuum‐assisted deliveries using the Kiwi Omnicup over 5 years. Primary outcomes were third‐ or fourth‐degree perineal tear, pH < 7.1, and subgaleal hematoma (SGH). AGAR, neonatal intensive care unit admission, cephalohematoma, Erb's palsy, third‐stage duration, and postpartum hemorrhage were secondary. Outcomes were compared between the occiput posterior (OP) and occiput anterior (OA) positions.ResultsThe study included 1960 patients. OP position was more likely to involve epidural analgesia (311 [82.5%] vs. 1216 [77%], P = 0.020), higher fetal head station (P = 0.001), higher percentage of cup detachments (121 cases [32.1%] vs. 307 [19.4%], P = 0.001), and longer procedure (5.5 ± 3.7 min vs. 4.7 ± 2.8 min, P = 0.001). OP was associated with umbilical cord pH < 7.1 (21 [5.5%] vs. 52 [3.9%], P = 0.032), NICU admissions (16 [4.2%] vs. 38 [2.4%], P = 0.049), SGH (18 [4.8%] vs. 38 [2.4%], P = 0.013), and high‐degree perineal tears (12 [3.2%] vs. 26 [1.7%], with borderline significance, P = 0.051). SGH and high‐grade tears remained significantly associated with OP position (P = 0.008 and P = 0.016, respectively) after adjusting for maternal age, nulliparity, diabetes, epidural anesthesia, preprocedure head station, and birth weight.ConclusionOP position is an independent risk‐factor for anal sphincter injury and SGH during vacuum‐assisted delivery.
Learning the Language of Oncology With Appa
Authors: Nam Rosa 
Published: July 2022

AbstractLike many children of immigrants, I have provided English language support to my Korean parents for as long as I remember. Up until this summer, I had no trouble translating or providing English support for my dad. But when my dad was diagnosed with metastatic brain cancer in May 2021, it felt like learning a new language, the language of oncology. In this common discourse reflection, I discuss how dad and I navigated the treatments to try and save his life.
A hyperspectral dataset of precancerous lesions in gastric cancer and benchmarks for pathological diagnosis
Authors: Zhang Ying , Wang Yan , Zhang Benyan , Li Qingli 
Published: July 2022

AbstractGastric cancer (GC) is one of the most common cancers worldwide. A lot of studies have found that early GC has good prognosis. Unfortunately, the diagnosis rate of early GC is suboptimal due to inadequate disease screening and the insidious nature of early lesions. Pathological diagnosis is usually regarded as the “gold standard” for the diagnosis of GC. However, traditional pathological diagnosis is tedious and time‐consuming. With the development of deep learning, computer‐aided diagnosis is widely used to assist pathologists for diagnosis. As conventional pathology, diagnosis is based on color images, it is not as informative as hyperspectral imaging, which introduces spectroscopy into imaging techniques. This article combines microscopic hyperspectral image (HSI) with deep learning networks to assist in the diagnosis of precancerous lesions in gastric cancer (PLGC). A large scale microscopic hyperspectral PLGC dataset with 924 effective scenes is built and self‐supervised learning is adopted to provide pretrained models for HSI. These pretrained models effectively improve the performance of downstream classification tasks. Furthermore, a symmetrically deep connected network is proposed to train with images from different imaging modalities and improve the diagnostic accuracy to 96.59%.
miR‐8485 alleviates the injury of cardiomyocytes through TP53INP1
Authors: Luo Xiuying , Xu Yanlin , Zhong Ze , Xiang Peng , Wu Xindong , Chong Aiguo 
Published: July 2022

AbstractMicroRNAs (miRNAs) feature prominently in regulating the progression of chronic heart failure (CHF). This study was performed to investigate the role of miR‐8485 in the injury of cardiomyocytes and CHF. It was found that miR‐8485 level was markedly reduced in the plasma of CHF patients, compared with the healthy controls. H2O2 treatment increased tumor necrosis factor‐α, interleukin (IL)‐6, and IL‐1β levels, inhibited the viability of human adult ventricular cardiomyocyte cell line AC16, and increased the apoptosis, while miR‐8485 overexpression reversed these effects. Tumor protein p53 inducible nuclear protein 1 (TP53INP1) was identified as a downstream target of miR‐8485, and TP53INP1 overexpression weakened the effects of miR‐8485 on cell viability, apoptosis, as well as inflammatory responses. Our data suggest that miR‐8485 attenuates the injury of cardiomyocytes by targeting TP53INP1, suggesting it is a protective factor against CHF.
Establishment of a time‐resolved immunoassay for acute kidney injury based on the detection of Kim‐1
Authors: Shaoxiong Zheng , Zhou Xiumei , Qin Yuan , Xiaomei Yu , Lingli Chen , Xiaobin Liu , Wang Yigang , Jianguang Gong , Shuijuan Shen , Huang Biao 
Published: July 2022

AbstractAimTo establish a highly sensitive time‐resolved fluorescence immunoassay (TRFIA) of kidney injury molecule‐1 (Kim‐1) and evaluate its clinical value in acute kidney injury (AKI).MethodsThe Kim‐1‐TRFIA was established by the double‐antibody sandwich method, and the method was evaluated. The established Kim‐1‐TRFIA was used to detect the concentration of Kim‐1 in the serum of healthy controls and patients with AKI.ResultsThe optimal coating antibody concentration and optimal Eu3+‐labeled antibody dilution ratio for Kim‐1‐TRFIA are 1 μg/ml and 1:140, respectively. The linear range is 42.71–4666.69 pg/ml. The intra‐ and inter‐assay coefficients of variation are <10%. The specificity of our Kim‐1‐TRFIA is acceptable. The recovery is between 95.14% and 102.84%. The concentration of Kim‐1 in the serum of patients with AKI is 126.50 ± 67.99 pg/ml, which is significantly higher than that in the serum of healthy controls (49.72 ± 16.40 pg/ml, p < 0.001). Staging patients with AKI by glomerular filtration rate shows that the serum concentration of Kim‐1 increases significantly with increasing disease severity (p < 0.05).ConclusionA highly sensitive Kim‐1‐TRFIA was established. With this immunoassay, a good differential diagnosis can be made, and healthy people and AKI patients can be differentiated by detecting the concentration of Kim‐1 in the serum. Moreover, the severity of AKI patients can be determined.
Performance evaluation of presepsin using a Sysmex HISCL‐5000 analyzer and determination of reference interval
Authors: Kang Taewon , Yoo Jeaeun , Choi Hyunyu , Lee Seungok , Jekarl Dong Wook , Kim Yonggoo 
Published: July 2022

AbstractBackgroundAnalytical evaluation of newly developed presepsin by a Sysmex HISCL‐5000 (Sysmex, Japan) automated immune analyzer was performed.MethodsFor evaluation, sepsis patient samples were collected before treatment in an emergency department. Precision, linearity, limit of blank/limit of detection, method comparisons, and reference intervals were evaluated. Method comparisons were performed using a PATHFAST immune analyzer (LSI Medience Corporation, Japan).ResultsPrecision using a 20x2x2 protocol for low (306 pg/mL) and high (1031 pg/mL) levels resulted in within‐laboratory standard deviation (95% confidence interval [CI]) and coefficient of variation (CV) %, which were as follows: 15.3 (13.1–18.7), 5.5% and 47.7, (40.5–58.1), 6.4%, respectively. Linearity using patient samples and calibrators were measured from 201 to 16,177 and 188 to 30,000 pg/mL, respectively. The regression equation was y = −23.2 + 1.008x (SE = 162.4) for low levels and y = 779.9 + 1.006x (SE = 668) for high levels. Method comparison by Passing–Bablock analysis was as follows: y = −209.77 + 1.047x (Syx = 335.3). The correlation coefficient (95% CI) was 0.869 (0.772–0.927) with statistical significance (p < 0.001). Reference intervals from 120 normal healthy subjects showed that 300 pg/mL was the cut off. Presepsin tended to show a higher value at higher ages and in males. Presepsin showed correlation with some parameters, and the correlation coefficient (p value) were as follows: hematocrit, 0.198 (0.03); eGFR (CKD‐EPI), −0.240 (0.0129); MDRD‐eGFR, −0.194 (0.048), respectively.ConclusionPresepsin measurement by HISCL‐5000 showed reliable performance. Further clinical studies are required for the diagnosis and prognosis of sepsis.
Enhanced adsorption of Pb(II) by phosphorus‐modified chicken manure and Chinese medicine residue co‐pyrolysis biochar
Authors: Chen Xi , Zhu Xiaoxuan , Fan Guangjian , Wang Xu , Li Haibo , Li Hui , Xu Xinyang 
Published: July 2022

AbstractThe pollution of lead (Pb (II)) to water resources is becoming more and more serious. It is always a difficult problem to find efficient and low‐cost adsorbents. Chicken manure (CM) and Chinese medicine residue (CMR) were modified with potassium dihydrogen phosphate (KH2PO4) and pyrolyzed to obtain a modified material (PBC) for the treatment of Pb(II) in an aqueous solution. A variety of characterization analysis results could prove that KH2PO4 was successfully introduced to PBC. By adjusting the initial pH, the zeta potential of PBC varies from −3.2 to −43.1 mV, it could be seen that PBC had excellent applicability in the broad range pH value (1.0–6.0). Experimental and model results showed that R2 of the pseudo‐first order kinetic model and the pseudo‐second order kinetic model are greater than 0.99, indicating that that physical and chemical adsorption played a significant role in Pb(II) removal by PBC. An adsorption isotherm analysis showed that the adsorption capacity n in this study is greater than 1, confirming that PBC has a good adsorption effect on Pb (II). R2 of the Langmuir model of PBC is 0.981, and its maximum adsorption capacity of Pb(II) could reach 599.4 mg/g. Environmentally friendly PBC could be used as an effective adsorbent to remove Pb(II) from aqueous systems.Research highlights
Chicken manure and Chinese medicine residue were converted into biochar to improve utilization.
The modified biochar exhibited extraordinary Pb(II) adsorption capacity.
Adsorption mechanisms: Surface complexation, ions exchange, coprecipitation, and so on.
Remained great Pb(II) removal efficiency at different pH and Pb(II) concentration.
N‐acetylglutamate synthase deficiency with associated 3‐methylglutaconic aciduria: A case report
Authors: Selvanathan Arthavan , Demetriou Kalliope , Lynch Matthew , Lipke Michelle , Bursle Carolyn , Elliott Aoife , Inwood Anita , Foyn Leanne , McWhinney Brett , Coman David , McGill Jim 
Published: July 2022

AbstractN‐acetylglutamate synthase (NAGS) deficiency is a rare autosomal recessive disorder, which results in the inability to activate the key urea cycle enzyme, carbamoylphosphate synthetase 1 (CPS1). Patients often suffer life‐threatening episodes of hyperammonaemia, both in the neonatal period and also at subsequent times of catabolic stress. Because NAGS generates the cofactor for CPS1, these two disorders are difficult to distinguish biochemically. However, there have now been numerous case reports of 3‐methylglutaconic aciduria (3‐MGA), a marker seen in mitochondrial disorders, occurring in CPS1 deficiency. Previously, this had not been reported in NAGS deficiency. We report a four‐day‐old neonate who was noted to have 3‐MGA at the time of significant hyperammonaemia and lactic acidosis. Low plasma citrulline and borderline orotic aciduria were additional findings that suggested a proximal urea cycle disorder. Subsequent molecular testing identified bi‐allelic pathogenic variants in NAGS. The 3‐MGA was present at the time of persistent lactic acidosis, but improved with normalization of serum lactate, suggesting that it may reflect secondary mitochondrial dysfunction. NAGS deficiency should therefore also be considered in patients with hyperammonaemia and 3‐MGA. Studies in larger numbers of patients are required to determine whether it could be a biomarker for severe decompensations.
Factors associated with high alanine aminotransferase (ALT) and cirrhosis in people living with HIV on combination antiretroviral treatment (cART) in the Asia‐Pacific
Authors: Rupasinghe Dhanushi , Choi Jun Yong , Yunihastuti Evy , Kiertiburanakul Sasisopin , Ross Jeremy , Ly Penh Sun , Chaiwarith Romanee , Do Cuong Duy , Chan Yu‐Jiun , Kumarasamy Nagalingeswaran , Avihingsanon Anchalee , Kamarulzaman Adeeba , Khusuwan Suwimon , Zhang Fujie , Lee Man Po , Van Nguyen Kinh , Merati Tuti Parwati , Sangle Sashikala , Oon Tek Ng , Tanuma Junko , Ditangco Rossana , Sim Benedict Lim Heng , Pujari Sanjay , Jiamsakul Awachana ,   TREAT Asia HIV Observational Database (TAHOD) of IeDEA Asia‐Pacific
Published: July 2022

AbstractLiver disease is a growing burden among people living with HIV (PLHIV) in resource‐limited settings. As an indicator of liver disease, risk factors of high alanine aminotransferase (ALT) and cirrhosis were assessed among PLHIV in the TREAT Asia HIV Observational Database (TAHOD). Patients on combination antiretroviral therapy (cART) with a pre‐cART ALT measurement and at least one follow‐up ALT measurement were included. Factors associated with high ALT (ALT levels > 5 times its upper limit of normal) were analyzed using repeated measure logistic regression over a 10‐year follow‐up period. Liver cirrhosis was defined as having an AST to Platelet Ratio Index score > 1.5, fibrosis‐4 score > 3.25, or a clinical diagnosis of cirrhosis. Cox regression analysis stratified by site was used to analyze factors associated with cirrhosis among those in follow‐up after 2015. Of 5182 patients, 101 patients (1.9%) had high ALT levels with hepatitis C virus (HCV) antibody positive (odds ratio [OR]: 4.98, 95% confidence interval [CI]: 2.82–8.77, p < 0.001) and ever high alcohol consumption (OR: 2.33, 95% CI: 1.00–5.46, p = 0.050) as likely factors. Among 6318 PLHIV in the liver cirrhosis analysis, 151 (2%) developed cirrhosis (incidence rate = 0.82 per 100 person‐years). Those HCV‐antibody positive (hazard ratio [HR]: 5.54, 95% CI: 3.75–8.18, p < 0.001) and had high alcohol consumption (HR: 2.06, 95% CI: 1.23–3.45, p = 0.006) were associated with liver cirrhosis. HCV‐antibody positive and high alcohol consumption are factors associated with high ALT. With raised ALT levels as a known factor associated with liver cirrhosis, greater efforts are required in managing ALT levels and reducing the risk of developing liver cirrhosis among those positive for HCV‐antibody and those who consume alcohol.
Bodily self‐recognition in patients with pathological embodiment
Authors: Candini Michela , Fossataro Carlotta , Pia Lorenzo , Vezzadini Giuliana , Gindri Patrizia , Galigani Mattia , Berti Anna , Frassinetti Francesca , Garbarini Francesca 
Published: July 2022

AbstractThe ability to discriminate between one's own and others' body parts can be lost after brain damage, as in patients who misidentify someone else's hand as their own (pathological embodiment). Surprisingly, these patients do not use visual information to discriminate between the own and the alien hand. We asked whether this impaired visual discrimination emerges only in the ecological evaluation when the pathological embodiment is triggered by the physical alien hand (the examiner's one) or whether it emerges also when hand images are displayed on a screen. Forty right brain‐damaged patients, with (E+ = 20) and without (E− = 20) pathological embodiment, and 24 healthy controls underwent two tasks in which stimuli depicting self and other hands was adopted. In the Implicit task, where participants judged which of two images matched a central target, the self‐advantage (better performance with Self than Other stimuli) selectively emerges in controls, but not in patients. Moreover, E+ patients show a significantly lower performance with respect to both controls and E− patients, whereas E− patients were comparable to controls. In the Explicit task, where participants judged which stimuli belonged to themselves, both E− and E+ patients performed worst when compared to controls, but only E+ patients hyper‐attributed others' hand to themselves (i.e., false alarms) as observed during the ecological evaluation. The VLSM revealed that SLF damage was significantly associated with the tendency of committing false alarm errors. We demonstrate that, in E+ patients, the ability to visually recognize the own body is lost, at both implicit and explicit level.
Occurrence of Streptomyces tauricus in mangrove soil of Mangalore region in Dakshina Kannada as a source for antimicrobial peptide
Authors: Karthik Yalpi , Kalyani Manjula Ishwara 
Published: July 2022

AbstractMicrobial resistance and deprivation of the effective drugs have become the foremost problem that propels to seek out for advanced approach. This concept initiated a need to search for more effective antimicrobial compounds from reliable sources. The Streptomyces is grouped under phylum Actinobacteria and are considered prolific producers of antibiotics, around 70% of presently available antibiotics are contributed by Streptomyces alone. In this study, Mangroves of the Mangalore Coast offered a unique source for screening Actinomyces group of microorganisms. We investigated on the four soil samples collected from Mangrove swamps of Mangalore, Karnataka, India. Based on their culture traits, the 18 distinct Actinomyces isolates were analyzed through a series of morphological and biochemical tests on starch casein nitrate (SCN) media. Culture biomasses were subjected for intracellular protein extraction through acetone precipitation method; the extracted proteins from each Actinomyces isolate were examined for antimicrobial activity against test organisms. The isolate ANTB‐YKMU4 showed potential antimicrobial activity against significant number of test organisms; Bacillus cereus, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, and Pseudomonas aeruginosa. The isolate ANTB‐YKMU4 through 16 s rRNA gene sequence analysis was identified as Streptomyces tauricus strain with GenBank accession no. MW785875.1. The S. tauricus further cultivated for efficient biomass growth on SCN media for subsequent protein extraction and purification by a series of Electrophoretic and chromatographic techniques. Thus, by intracellular extractions from S. tauricus resulted in the identification of peptide with a molecular weight of 266 Da that was characterized by LC‐MS.
Impact of expired stock on antimicrobial surveillance in rural hospitals: implications and challenges for stewardship
Authors: Macauley Georgia , Penny Michelle , McNeil Vicki , Gustafsson Tanja , Hillock Nadine 
Published: July 2022

AbstractBackgroundQuantitative surveillance of antimicrobial use is a valuable tool used to support antimicrobial stewardship, identify overprescribing, monitor unexpected changes in usage, and assess the impact of interventions to improve prescribing. Smaller, more remote hospitals face many challenges in conducting effective antimicrobial surveillance.AimTo investigate the impact of expired stock on reported antimicrobial usage rates in smaller, more remote hospitals.MethodAntimicrobial usage rates (defined daily doses [DDDs] per 1000 occupied bed days [OBDs]) were calculated using monthly dispensing data and hospital activity data from 12 rural South Australian facilities for the period January 2018 to December 2020. Usage rates were re‐calculated, excluding expired stock, to estimate the impact expired stock had on reported usage rates and to quantify stock wastage.ResultsBetween 2018 and 2020, the average monthly aggregate usage rate for all 12 hospitals was 650 DDD/1000 OBDs, with the exclusion of expired stock resulting in an average monthly reduction of 37 DDD/1000 OBDs (5.7%). Analysis by the Australian Institute of Health and Welfare peer group demonstrated that the exclusion of expired stock reduced average monthly usage rates by 6.0% for Public Acute Group C sites and 10.6% for Public Acute Group D and Very Small Hospitals.ConclusionReplacement of expired stock may account for a substantial proportion of perceived antimicrobial usage in rural and remote hospitals, particularly for agents infrequently prescribed. Pharmacy distribution data is a surrogate measure for actual patient consumption; utilisation reports for smaller rural facilities should be interpreted with acknowledgment of the challenges of stock management in remote locations.
Methods for quality evaluation of sweet cherry
Authors: Ricardo‐Rodrigues Sara , Laranjo Marta , Agulheiro‐Santos Ana Cristina 
Published: July 2022

AbstractSweet cherry (Prunus avium L.) is a highly valued fruit, whose quality can be evaluated using several objective methodologies, such as calibre, colour, texture, soluble solids content (SSC), titratable acidity (TA), as well as maturity indexes. Functional and nutritional compounds are also frequently determined, in response to consumer demand. The aim of the present review is to clarify and establish quality evaluation parameters and methodologies for the whole cherry supply chain, in order to promote easy and faithful communication among all stakeholders. The use of near‐infrared spectroscopy (NIRS) as a non‐destructive and expeditious method for assessing some quality parameters is discussed. In this review, the results of a wide survey to assess the most common methodologies for cherry quality evaluation, carried out among cherry researchers and producers within the framework of the COST Action FA1104 ‘Sustainable production of high‐quality cherries for the European market’, are also reported. The standardisation of quality evaluation parameters is expected to contribute to the preservation and shelf‐life extension of sweet cherries, and the valorisation of the whole supply chain. For future studies on sweet cherry, we put forward a proposal regarding both sample size and the tests chosen to evaluate each parameter. © 2022 Society of Chemical Industry.