bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of tyrosine hydroxylase within dapoxetine-assisted therapy against premature ejaculation
Authors: Liu Xi , Zhu Tianle , Gao Pan , Gao Jingjing , Gao Rui , Jiang Hui , Zhang Xiansheng 
Published: November 2022

Abstract
Background: There are several investigations that have revealed that cerebral dopamine (DA) plays a pivotal role in the occurrence of premature ejaculation (PE). Although tyrosine hydroxylase (TH) is an essential enzyme for the synthesis of DA, only few investigations have describedthe role of TH in regulation mechanisms for ejaculation till now. To investigate whether there is a correlation between TH expression level in the brain and different ejaculation behavior in rats. Then explore whether the TH expression in the brain will change after acute dapoxetine treatment in rats with Rapid ejaculation.
Methods and Results: Male S-D rats were separated into three groups based on their ejaculation frequency: Rapid, Normal, and Sluggish. Expression level of DA in the brain was determined by enzyme-linked immune sorbent assay (ELISA) kit, TH expression level in the brain was determined by immunohistochemistry and Western Blot (WB) techniques. Among the three groups, DA and TH expression level were the highest in the Rapid ejaculation group, while the lowest was the Sluggish ejaculation group. The results also showed that TH level was positively associated with ejaculation frequency (r=0.8038, P<0.001) and negatively associated with ejaculation latency (r=-0.6199, P<0.05). Furthermore, acute dapoxetine therapy in rats with Rapid ejaculation downregulated TH level in the brain.
Conclusion: Changes in ejaculation behavior were significantly linked with TH level. Upregulated TH in selected brain regions related with ejaculation could cause rapid ejaculation. The effect of dapoxetine in prolonging ejaculation could be related to TH downregulation within the brain.
Comprehensive Assessment of Isoform Detection Methods for Third-Generation Sequencing Data
Authors: Liu Wanlu , Su Yaqi , Yu Zhejian , Xue Ziwei , Guo Yixin 
Published: November 2022

Abstract

The advancement of Third-Generation Sequencing (TGS) techniques managed to increase the sequencing length to several kilobases, which leads to a bright future for completely reserving alternative splicing (AS) events and isoform expressions. In recent years, many computational methods for isoform detection from long-read sequencing data have been developed and published. However, there is no prior comparative study that systemically evaluates the performance of the software implemented with different algorithms. Here we benchmarked nine methods implemented in seven computational tools that can identify isoform structures from TGS RNA sequencing data and analyzed their performances from various aspects using both simulated datasets produced by an in-house simulator and previously published experimental data. Our results comprehensively demonstrate the relative effectiveness of the approaches and provide guidance as well as recommendations for future research on AS analysis and further improvement of the tools for isoform detection using TGS data.
Bioinformatics-based prognostic analysis of non-small cell lung cancer
Authors: Zhao Ming-Li , Li Yun-Xia , Hao Shu , Wu Na , Zhou Wen-Ding , Xun Xiang-Han , Bi Qing 
Published: November 2022

Abstract
Background: With 75% of patients with non-small cell lung cancer (NSCLC) being found at an intermediate to advanced stage and a five-year survival rate of only 7%-17%, there is a need to find ways to improve the five-year survival rate of patients with NSCLC for prognosis. We used bioinformatics analysis of NSCLC samples from The Cancer Genome Atlas (TCGA) database to screen for differential genes and find multigene models for risk assessment of NSCLC patients, which is important for individualised clinical treatment and prognosis of NSCLC patients. Considering the limitations of the samples in this study, further validation in clinical and basic experiments is needed.
Methods and results: The 519 samples associated with NSCLC were screened using bioinformatics in TCGA database, and the differential genes were selected by univariate analysis and Least Absolute Shrinkage and Selection Operator (LASSO) regression model. The most effective multi-gene model was selected by multi-gene analysis, and the validity of the multi-gene model was verified by survival analysis and Receiver Operating Characteristic (ROC) curves, and finally by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and The mRNA differential genes were enriched KEGG and Gene Ontology (GO) databases. The GO enrichment analysis showed that the differential genes were associated with extracellular structural tissues, external encapsulated structural tissues and extracellular matrix tissues. enrichment indicated that the differential genes were associated with histidine metabolism, calcium signalling pathways and cytokine-cytokine receptor interactions, among others. In conclusion, a polygenic model consisting of 22 genes can be used as a tool for the prognosis of NSCLC.
Conclusion: Polygenic models provide an ideal and effective approach to the prognosis of NSCLC. In this study, we screened a set of multigene models as a risk assessment model for the prognosis of NSCLC.
A savings intervention to reduce men's engagement in HIV risk behaviors: study protocol for a randomized controlled trial
Authors: Egbe Teniola I. , Omollo MPH Ouma Dan , Wesonga MScE Julius Oduor , Bair MS Elizabeth F. , Chakrabarti Averi , Putt PhD Mary E. , Celum Connie L. Celum L. , Camlin Carol S. , Napierala Sue , PhD  , Thirumurthy Harsha 
Published: November 2022

Abstract
Background
In much of eastern and southern Africa, the incidence of HIV and other sexually transmitted infections (STIs) remains high despite the scale-up of promising biomedical and behavioral interventions. Studies have documented the crucial role of transactional sex – the exchange of money, material support or goods in, sexual relationships – and heavy alcohol use in driving HIV/STI incidence and influencing men’s and women’s health outcomes. Existing policy responses to this challenge have largely focused on interventions to reduce women’s engagement in transactional sex such as vocational training and cash transfers for economic empowerment. However, the effectiveness of these interventions has been hindered by the relative lack of programs targeting men’s behavior. There is a vital need for interventions that can reduce men’s engagement in risky behaviors that increase HIV/STI risk. We describe here a protocol for an economic intervention to reduce men’s engagement in HIV risk behaviors in Kenya.
Methods
We will conduct a randomized controlled trial among income-earning men in Kenya who are aged 18–39 years and self-report alcohol use and engagement in transactional sex. The study will enroll 1,500 participants and randomize them to a control group or savings group. The savings group will receive access to a savings account that includes lottery-based incentives to save money regularly, opportunities to develop savings goals/strategies, and text message reminders about their saving goals. The control group will receive basic health education. Over a period of 24 months, we will collect qualitative and quantitative data from participants and a subset of their female partners. Participants will also be tested for HIV and other STIs at baseline, 12, and 24 months.
Discussion
The findings from this study have the potential to address a missing element of HIV/STI prevention efforts in Sub-Saharan Africa by promoting upstream and forward-looking behavior and reducing the risk of acquiring HIV/STIs in a high HIV/STI burden setting. If this study is effective, it is an innovative approach that could be scaled up and could have great potential for scientific and public health impact in Kenya.
Trial registration
: Registered on ClinicalTrials.gov on May 23, 2022. Link: https://clinicaltrials.gov/ct2/show/NCT05385484. Registration number: NCT05385484
FARSB Serves as a Novel Hypomethylated and Immune Cell Infiltration Related Prognostic Biomarker in Hepatocellular carcinoma
Authors: Zhen Jing , Pan Jingying , Zhou Xuanrui , Yu Zichuan , Jiang Yike , Gong Yiyang , Ding Yongqi , fang Jiayu , Liu Yue , Huang Da 
Published: November 2022

Abstract
Purpose
Hepatocellular carcinoma (HCC) is a prevalent tumor with high morbidity, and an unfavourable prognosis. FARSB is an aminoacyl tRNA synthase, and plays a key role in protein synthesis in cells. Furthermore, previous reports have indicated that FARSB is overexpressed in gastric tumor tissues and is associated with a poor prognosis and tumorigenesis. However, the function of FARSB in HCC has not been studied.
Results
The results showed that FARSB mRNA and protein levels were upregulated in HCC and were closely related to many clinicopathological characteristics. Besides, according to multivariate Cox analysis, high FARSB expression was linked with a shorter survival time in HCC and may be an independent prognostic factor. In addition, the methylation level was negatively associated with the expression of FARSB. Furthermore, enrichment analysis showed that FARSB was related to the cell cycle. And TIMER analysis revealed that the FARSB expression was closely linked to tumor purity and immune cell infiltration. The TCGA and ICGC data analysis suggested that FARSB expression is greatly related to m6A modifier related genes. Potential FARSB-related ceRNA regulatory networks were also constructed. What’s more, based on the FARSB-protein interaction network, molecular docking models of FARSB and RPLP1 were constructed. Finally, drug susceptibility testing revealed that FARSB was susceptible to 38 different drugs or small molecules.
Conclusion
FARSB can serve as a prognostic biomarker for HCC and provide clues about immune infiltration, and m6A modification.
Toothpaste-related interests of Google users from different countries
Authors: Profio Bruna Di , Lotto Matheus , Aguirre Patricia Estefania Ayala , Villar Cristina Cunha , Romito Giuseppe Alexandre , Braga Mariana Minatel , Cruvinel Thiago , Pannuti Cláudio Mendes 
Published: November 2022

Abstract
Background
The Internet is a growing source of knowledge and can provide information about oral health. This ecological study aimed to characterize the interests of Google users on toothpaste in different countries.
Methods
This longitudinal retrospective study analyzed the toothpaste-related interest of Google users from ten distinct countries between January 2004 and December 2020. The monthly variation in Relative Search Volume (RSV) and the main related queries were determined using Google Trends. Autoregressive Integrated Moving Average (ARIMA) forecasting models were built to establish the predictive RSV values for mouthwash for an additional 12 months. Auto-correlation plots and a general additive model (GAM) were used to diagnose trends and seasonality in RSV curves. Additionally, the influence of social isolation during the COVID-19 pandemics was analyzed.
Results
Although not detected by ACF and PACF analyses, the heuristic analysis showed an increase in the interest in toothpaste-related information in all countries, with a stable trend observed in the 12-month forecasts, except for the increases in the United Kingdom and South Africa. Also, GAM analyses demonstrated a non-significant monthly or quarterly seasonal influence on data. In addition, the social isolation of the COVID-19 pandemic did not influence the online information-seeking behavior of Google Search users linked to this topic.
Discussion
The heuristic analysis of the time series indicated an increase in the interest of Internet users in toothpaste between January 2004 and December 2020. This increase may reflect the population's growing autonomy for solving their own health problems. The Internet seems to be the most popular source of information. As such, this information may enable the implementation of specific public oral health policies and oral-care communications based on the population´s needs. Along these lines, information monitoring and improvements in oral health literacy may contribute to the reduction of currently existing social and economic barriers.
Conclusion
The interest of Google Search users in information about toothpaste increased in all ten countries. These trends were not influenced by the outbreak of the COVID-19 pandemic in most countries.
Diagnostic Value of ABO Blood Group Combined with Tumor Markers in Gastric Cancer
Authors: Liu Yuhuan , Chen Shasha , Shen Weina , Qu Xiaodong , Li Songbo , Shi Yongquan 
Published: November 2022

Abstract
Background
Blood type is related to the occurrence and development of gastric cancer. The aim of this study is to explore the value of combined detection of ABO blood group and tumor markers in the diagnosis of gastric cancer.
Methods
A total of 3650 gastric cancer patients treated in our center from January 2015 to December 2019, and 5822 controls were recruited, and divided into training set and validation set according to 7:3. The clinicopathological characteristics were recorded. Receiver operating characteristic curve (ROC curve) was used to evaluate the diagnostic value of tumor markers and their combined blood type for gastric cancer. The results were validated using another group of people (validation group).
Results
In both the training set and the validation set, the levels of tumor markers in gastric cancer patients were higher than those in the healthy control group (P < 0.05). The distribution of blood group in gastric cancer group was: A > B > O > AB, while that in healthy control group was: B > O > A > AB. Type B blood (AUC = 0.850,95%CI 0.837–0.863, P = 0.0006) and type AB blood (AUC = 0.837,95%CI 0.812–0.860, P = 0.0439) could improve the diagnostic value of CEA in gastric cancer.
Conclusion
The detection of blood group combined with tumor markers can improve the sensitivity and specificity for the diagnosis of gastric cancer, which has a high reference value for the diagnosis of gastric cancer patients, and is worthy of further clinical study.
Local characteristics of molecular epidemiolgy of Acinetobacter baumannii in Jilin Province (northeast China)
Authors: You Qingsong , Du Xue , Hu Nannan , Zhang Yusi , Zhang Na , Wang Fusheng , Li Jinghua , Sun Yanbo , Wang Fang , Shi Hongyan 
Published: November 2022

Abstract
A. Baumannii is an opportunistic nosocomial pathogen which have severe antibiotic-resistant. However, the epidemiology is less clearly understood in Jilin province and China. Thus, 89 A. baumannii isolates from a single hospital in Jilin province between 2013-2017 were performed by multi-locus sequence typing (MLST), and all Chinese strains with definite STs in MLST database were analyzed by silico phylogenetic tools. A total of 22 Sequence types (STs) in Jilin were identified and 10 STs were found to be novel. The top three predominant sequence types are ST195 (n=34, 38.2%), ST208 (n=14, 15.7%) and ST540 (n=13, 14.6%). ST369 is predicted to be group founder and ST195, ST540 are subgroup founders of the majority STs in Jilin Province. The majority of all Jilin isolates showed clonality and close relationship with the majorities from other regions of China. But occupation of individual STs in Jilin were different from that of other domestic regions. Excepting domestic transmission, some newly discovered singleton showed close relationship with strains from other countries, which suggest that nation-cross transmission is one of important origin of Jilin strains. And the aggregation trend and close genetic relationship of dominant STs proved that predominant STs continue to mutate in the process of transmission. Drug resistance facilitated transmission of Jilin A.baumannii isolates because more than 94% of isolates are resistant to at least one carbapenem and the STs with strong resistance to carbapenems usually has more isolates. In conclusion, high diversity and different occupation of STs, and occupation of novel STs proved that epidemiology of A. baumannii in Jilin has special regional characteristics, and drug resistance facilitated transmission of domestic strains and foreign strains.
Drug-resistant extracellular vesicles: a reliable biomarker for predicting therapeutic response in patients with triple-negative breast cancer receiving neoadjuvant chemotherapy
Authors: Kim Min Woo , Lee Hyojung , Lee Suji , Moon Sol , Kim Young , Kim Joon Ye , Kim Seung Il , Kim Jee Ye 
Published: November 2022

AbstractBackground:Predicting tumor response for neoadjuvant chemotherapy (NAC) is critical for evaluating prognosis and deciding the treatment strategy in patients with breast cancer; however, there are no reliable circulating biomarkers that can assess therapeutic responses appropriately and effectively. Therefore, we aimed to validate the clinical feasibility of extracellular vesicles (EV) collected through liquid biopsy as biomarkers for predicting tumor response after NAC.Methods:Drug-resistant sublines were generated from a panel of triple-negative breast cancer (TNBC) cell lines, including HCC1395, MDA-MB-231, and MDA-MB-468. Comparison with parental cells suggested candidate biomarkers to detect resistance to chemotherapeutic drugs. EVs were isolated from cultured cells and plasma samples collected before NAC from breast cancer patients (N=36). The EVs were then coupled to immuno-beads to allow the quantification of drug resistance-related EV markers by flow cytometry.Results:Drug-resistant TNBC cell lines were stably established showing specific morphology and rapidly growing features. Among the differentially expressed gene profiles between parental and drug-resistant cell lines, drug efflux transporters, such as multidrug resistance protein 1 (MDR1), MDR-associated protein 1 (MRP1), and breast cancer resistance protein (BCRP) were highly expressed in resistant cell lines. Drug efflux transporters were identified in not only cell lines but also EVs released from parental cells with immuno-affinity-based EV isolation. Among 20 TNBC patients, the expression of drug-resistant markers in EVs from the patients with the residual disease was relatively high compared to that of patients with pathological complete response (pCR). The optimal combination of drug-resistant EV markers was significantly efficient in predicting patients showing resistance to NAC and represented the best performance with 93% sensitivity, 80% specificity, and 0.93 area under the receiver operating characteristic curve.Conclusions:The combination of drug-resistant EV markers is effective in predicting the therapeutic response of breast cancer patients who are treated with NAC.
Successful First Use of a Phage Endolysin for Treatment of
Chronic Pelvic Pain Syndrome/Chronic Bacterial Prostatitis
Authors: Stevens Roy , Zhang Hongming , Kajsík Michal , Płoski Rafal , Rydzanicz Malgorzata , Sabaka Peter , Sutovský Stanislav 
Published: November 2022

Abstract
Chronic prostatitis (CP) is a common inflammatory condition of the prostate that is estimated to effect 2-10% of the world’s male population. It can manifest as perineal, suprapubic, or lower back pain and urinary symptoms occurring with either recurrent bacterial infection (chronic bacterial prostatitis [CBC]) or in the absence of evidence of bacterial infection (chronic pelvic pain syndrome [CPPS]). Here, we report a case of a 39-year-old patient displaying symptoms of CP in which a bacteriophage-derived muralytic enzyme (endolysin) was successfully used to resolve the disease. Bacteriological analysis of the patient’s prostatic secretion and semen samples revealed a chronic Enterococcus faecalis prostate infection, supporting a diagnosis of CBP. The patient’s E. faecalis strain was resistant to several antibiotics and developed resistance to others during the course of treatment. Previous treatment with multiple courses of antibiotics, bacteriophages, probiotics, and immunologic stimulation had failed to achieve long term eradication of the infection or lasting mitigation of the symptoms. A seven-day course of treatment with the endolysin resulted in the elimination of the E. faecalis infection to below culturally detectable levels, and the abatement of symptoms to near normal levels. Furthermore, during the endolysin treatment, the patient experienced no untoward reactions. The present report represents the first clinical demonstration of the efficacy of a bacteriophage endolysin in the management of a recalcitrant systemic infection.
Caries activity tests and associated factors among 3- to 5-year -old preschool children in Zhao County,Hebei province,China
Authors: Lin Xiuyan , Wang Yuan , Ma Zhe , Cao Fengdi , Zhao Caiyun , Wang Xinfeng , Shi Hong 
Published: November 2022

Abstract
Background:This study aims to investigate the caries activity (CA) and its caries status in children aged 3–5 years in Zhao County, Hebei province, China and to explore the related influencing factors. In addition, to analyze the diagnostic value of Cariostat caries activity test in early childhood caries (ECC).
Methods:Cariostat caries activity test and oral examination were performed on children aged 3–5 years old in Zhao county, Hebei province by a stratified sampling and to evaluate the caries status, decayed-missing-filled teeth (dmft), caries prevalence, and significant caries index (SiC). A questionnaire survey was used to analyze the CA-related influencing factors and the receiver operating characteristic curve (ROC) was to evaluate the diagnostic value of Cariostat caries activity test in children with ECC.
Results: A total of 693 children aged 3–5 years old were selected in this study. The mean dmft score was 7.6 ± 5.0, and the prevalence of deciduous dental caries was 90.5%. There is a positive correlation between the dmft score and CA value (r = 0.586, P < 0.001), and CA and caries prevalence increased with age (P < 0.05). The results of logistic regression analysis shows that the possible risk factors for high CA included: low educational level of mothers, night-weaning at a later age, high frequency of sugary beverages consumption and low frequency of dental visits. The higher the frequency of vegetables consumption, the lower the risk of CA. The area under the ROC curve was 0.924, and the sensitivity and specificity were 87.40% and 91.17%, respectively.
Conclusions:The caries status of preschool children aged 3–5 years old in Zhao county of Hebei province was serious. The higher the mean dmft of children, the greater the CA value. Mother's educational level and children's diet and lifestyle habits were closely related to CA. The Cariostat caries activity test has good efficacy in diagnosing ECC with high sensitivity and specificity.