bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of apical periodontitis on the systemic levels of C-reactive protein
Authors: Souza Erick , Valois Érica , Cruz Maria Carmem da , Filho Etevaldo Maia , Sluis Luc van der 
Published: August 2022

Abstract
Objectives This study explores the potential relation between apical periodontitis (AP) and C-reactive protein (CRP) levels, a laboratorial surrogate of systemic inflammation. Material and Methods 144 consecutive patients were selected, after exclusion criteria, from a University Dental Clinic. A full mouth series of periapical radiographs was taken, and patients were allocated to two groups (n = 77): AP-present or AP-absent. In the AP-present group, the number of affected teeth/per patient was counted. Patient’s CRP levels were immediately measured, using the Quickread CRP®. CRP cut-off level to indicate for systemic consequences was set at ≥ 8mg/L, and CRP readings were categorized at or below this level for statistical evaluation. A Chi-square test verified whether the frequencies of CRP levels (< 8 mg/L and ≥ 8 mg/L) differed between the groups with and without AP. The odds of an AP-positive patient to present ≥ 8mg/L CRP levels was also calculated. A Spearman correlation verified whether the number of affected teeth influence the CRP levels in the AP-present group. Results The frequency of AP-free patients reading high CRP levels (≥ 8 mg/L) (11.68%) is significantly lower compared to the AP-present (25.97%) (Chi-square, X2 = 5.1404, P = 0.023). AP-positive patients displayed 2.65-fold higher risk for CRP ≥ 8 mg/L (OR = 2.65, 95%CI: 1.12–6.28). There was no correlation between the number of AP-affected teeth and the two categories of CRP blood levels (Spearman, rs=0.129, P = 0.26). Conclusions A potential systemic impact of endodontically compromised teeth has been found since AP-positive patients display 2.65-fold higher odds of increased CRP levels. Clinical Significance Patients with apical periodontitis present about 2.6 times odds to display elevated systemic levels of C-reactive protein, a serum marker that may be considered a risk factor to generate coronal heart diseases.
Association between Childhood Trauma and Medication Adherence among Patients with Major Depressive Disorder: The Moderating Role of Resilience
Authors: Wang Hongqiong , Liao Yuhua , Guo Lan , Zhang Huimin , Zhang Yingli , Lai Wenjian , Teopiz Kayla M. , Song Weidong , Zhu Dongjian , Li Lingjiang , Lu Ciyong , Fan Beifang , McIntyre Roger S. 
Published: August 2022

Abstract
Background
Medication adherence among patients with major depressive disorder (MDD) is a major and modifiable problem for health care systems. Childhood trauma is considered a vital factor that might be helpful to the early risk assessment of suboptimal adherence. We aimed to comprehensively explore the associations between different types of childhood trauma and medication adherence among patients with MDD, and to test whether measure of resilience has moderating effects on the foregoing associations.
Methods
Participants were from the Depression Cohort in China (ChiCTR registry number 1900022145), 282 patients with MDD completed the 12 weeks study. The diagnosis of MDD was assessed by trained psychiatrists using the Mini-International Neuropsychiatric Interview (M.I.N.I.). Childhood trauma was evaluated using the Childhood Trauma Questionnaire-28 item Short Form (CTQ-SF), resilience was evaluated using the Connor-Davidson Resilience Scale (CD-RISC). Demographic characteristics, depression symptoms, anxiety symptoms, suicidal ideation, suicidal attempt, insomnia symptoms, and painful somatic symptoms were also investigated. Participants were divided into groups of optimal and suboptimal adherence based on their scores on the Medication Adherence Rating Scale, and factors associated with medication adherence using univariate and multivariate logistic regression, and stratified analyses were evaluated.
Results
A total of 234 participants (83%) reported suboptimal medication adherence. After adjusting for covariates, CTQ total scores (AOR = 1.04, 95%CI = 1.01–1.06), CTQ measures of sexual abuse (AOR = 1.23, 95%CI = 1.07–1.42), and CTQ measures of physical neglect (AOR = 1.14, 95%CI = 1.04–1.12) were all associated with an increased likelihood of suboptimal adherence. There were significant moderating effects of resilience on the associations of childhood trauma (P = 0.039) and physical neglect (P = 0.034) with medication adherence. The stratification analyses showed that CTQ total scores and CTQ measures of physical neglect were independently associated with an increased risk of suboptimal adherence among patients with MDD with low-resilience or medium-resilience, while not significantly associated with suboptimal adherence in those with high-resilience.
Conclusion
Obtaining a history of childhood trauma and assessing resilience may help identify patients with suboptimal adherence when providing MDD pharmacotherapy. Psychiatrists may consider enhancing resilience to cope with the adverse effects of childhood trauma on medication adherence.
Excitation of terahertz surfacemagnetoplasmons by nonlinear mixing of laser and its second harmonic on a rippled surface of n type semiconductor
Authors: Srivas Rohit Kumar , Panwar A. 
Published: August 2022

Abstract
We investigated the excitation of terahertz (THz) surface magneto plasmons(SMPs) by nonlinear mixing of laser and its frequency shiftedsecond harmonic on a rippled surface of n-type semiconductor-free spaceinterface. Obliquely incident p-polarized lasers exert a nonlinear ponderomotiveforce on free electrons of n-type semiconductor. Nonlinearponderomotive force induce the oscillatory velocities at frequencies 2ω1and (ω1 −ω2). These oscillatory velocities beat the modulated electrondensity nq to get the charge density perturbation at (2ω1, 2k1z + q)and (ω1 − ω2, k1z − k2z + q). Perturbed charge density couples withthe linear oscillatory velocities to produce a nonlinear current density,which resonantly derives THz SMPs at frequency ω = 2ω1 − ω2 andpropagation constant kz = 2k1z − k2z + q. Here, q provides theextra wave number for the phase matching condition. The efficiencyof THz SMPs wave attained up to 9% and amplitude of THz SMPscontrolled by the electron cyclotron frequency ωce and incident angle θ.
Computational design of new drugs for binding ⍺-amylase as candidates for treating diabetes
Authors: Alp Meryem , Misturini Alechania , Sastre German , Gálvez-Llompart Maria 
Published: August 2022

Abstract
Type-2 diabetes is a pandemic metabolic disorder affecting almost 200 million people globally, and this figure is expected to rise gradually to 642 million by 2040. α-amylase is a digestive enzyme that causes postprandial hyperglycemia and blood glucose levels rise. Inhibiting the α-amylase enzyme, it could help to reduce hyperglycemia, obesity, and overweight conditions by reducing postprandial levels of glucose. In the present study, the identification of potential α-amylase inhibitors is explored as a potential strategy for treating type-2 diabetes mellitus. A new computationally driven approach aimed at identifying novel α-amylase inhibitors based on molecular docking was carried out. The interactions of potential drugs with the enzyme’s active site were investigated and compared with the contacts established by acarbose (a reference drug for α-amylase inhibition) in the crystallographic structure 1B2Y. For this active site characterization, both molecular docking and molecular dynamics simulations were performed, and the residues involved in the α-amylase–acarbose complex were considered to analyse the potential drug's interaction with the enzyme. Two potential α-amylase inhibitors (AN-153I105594 and AN-153I104845) have been selected following this computational strategy. Both compounds established a large number of interactions with key binding site α-amylase amino acids and obtained a comparable docking score with respect to the reference drug (acarbose).
LncRNA NEAT1 exacerbates sepsis-induced acute lung injury via targeting miR-26a-5p/ROCK1 axis
Authors: Fan Xiu-Ying , Tang Li-Bin , Ma Zhong-Xu , Shen Han-Zhang , Qi Fei , Xia Jia-Wei 
Published: August 2022

Abstract
Background: Acute lung injury (ALI) is a severe disease of respiratory system, which one of the most common causes is sepsis induction. Cell apoptosis and inflammation contribute to the progression of ALI and lncRNAs play crucial roles in ALI. Hence, this study is to unearth the specific mechanism of NEAT1.Methods: BEAS-2B cells was exposed to lipopolysaccharide (LPS) to construct cell model of sepsis-induced ALI. The gene and protein expression were evaluated using qRT-PCR and western blot. Cell viability was determined by CCK-8. Cell apoptosis was detected utilizing flow cytometry. The secretion of inflammatory factors was assessed using ELISA. The interconnections among NEAT1, miR-26a-5p and ROCK1 were validated using starbase, luciferase assay and RIP.Results: LPS treatment elevated NEAT1 and ROCK1 levels while reduced miR-26a-5p level in BEAS-2B cells. Besides, LPS treatment augmented cell apoptosis and enhanced inflammatory cytokine secretion, whereas NEAT1 silencing could reversed LPS-mediated these influences in BEAS-2B cells. Mechanistically, NEAT1 positively mediated ROCK1 expression through targeting miR-26a-5p. Moreover, miR-26a-5p inhibitor offset NEAT1 depletion-mediated suppressive influences on cell apoptosis and inflammation. ROCK1 upregulation attenuated the inhibitory impacts generated by miR-26a-5p overexpression on cell apoptosis and inflammation. Conclusion: Our results revealed NEAT1 could reinforce LPS-induced cell apoptosis and inflammatory response through repressing miR-26a-5p/ROCK1 axis, thereby aggravating ALI caused by sepsis. Our data suggested NEAT1, miR-26a-5p and ROCK1 might be biomarkers and targets genes for alleviating sepsis-induced ALI.
Evaluation of Intraoperative Navigation-assisted Thoracoscopic Oesophagectomy Outcomes in the Patient's Semi-prone Position
Authors: Khorobykh Тatyana , Dydykin Sergey , Mishchenko Natalya , Kapitonova Мarina , Gupalo Sergey , Kytko Olesya , Bogoyavlenskaya Тatyana , Agadzhanov Vadim , Salikhov Рashad 
Published: August 2022

Abstract
Video-assisted thoracoscopic esophagectomy for oesophageal cancer involving the thoracic region has certain advantages. However, thoracoscopic instrument manipulations performed in the narrow confines of the posterior mediastinum carry the risk of intraoperative injury to other vital structures.Purpose of the studyTo improve surgical treatment outcomes of thoracic oesophageal cancer patients using topographic anatomical navigation.Materials and methodsThe study included 23 patients with stages II-III middle and lower thoracic oesophageal cancer (TOC), according to TNM-8. All of them underwent thoracoscopic oesophagectomy in the semi-prone position. While the 1st group of patients (n=15) underwent conventional surgery, in the 2nd group of patients (n= 8), our algorithm of sequential visual navigation based on topographic and anatomical landmarks was employed.ResultsIn the 1st group,4 patients (26.7%) developed intraoperative complications. Out of these four, in 3 patients, injury of the thoracic lymphatic duct complicated with chylothorax development was diagnosed, requiring reoperations; in one patient, the surgery caused damage to the thoracic aorta. Unfortunately, two patients out of these four died.In contrast, there were no intraoperative complications or deaths in the 2nd group. In addition, the algorithm allowed the removal of 18% more lymph nodes, reduction in the incidence of postoperative pneumonia by 25%, the operation time by 25±15 minutes, and the length of stays by 3±2 days, compared with the 1st group of patients. ConclusionTopographic anatomical navigation can reduce the incidence of intra- and postoperative complications, effectively guide lymph node dissection, and shorten hospital stay.
Comparison of Quality of Life in patients with advanced chronic kidney disease undergoing haemodialysis, peritoneal dialysis and conservative management in Johannesburg, South Africa: a cross-sectional, descriptive study
Authors: Mathew Neelu , Davies Malcolm , Kaldine Feroza , Cassimjee Zaheera 
Published: August 2022

Abstract
Introduction:
Mental health and quality of life are under-appreciated clinical targets which affect patient and modality survival. Lack of dialysis slot availability in the resource-constrained public health sector in South Africa, results in assignment to treatment modalities without regard to effects on these parameters. We assessed the effect of dialysis modality, demographic and laboratory parameters on mental health and quality of life measurements.
Methods
Size-matched voluntary cohorts were recruited from patients on haemodialysis (HD), peritoneal dialysis (PD), and patients on conservative management (with an estimated glomerular filtration rate below 20mL/min/1.73m2), between September 2020 and March 2021. Responses to self-administered Hospital Anxiety and Depression Scale (HADS) and Kidney Disease Quality of Life Short Form 36 (KDQOL-SF36) questionnaires and demographic and baseline laboratory parameters were compared between treatment modalities using the Student t-test and Pearson Chi-square test. Linear regression was used to test for independent effect where significant difference was observed.
Results
HADS anxiety score was highest (p < 0.001) and KDQOL-SF36 emotional wellbeing was poorer in HD (p < 0.001). Social functioning (p = 0.011) and physical limitation due to pain (p = 0.030) were poorer in PD. Unemployment (p = 0.044) was more frequent in HD; fewer PD patients required a social support grant (p = 0.008). Significant independent effect was found for age (p = 0.009), employment (p = 0.007), and haemoglobin (Hb) (p = 0.025) on anxiety; HD worsened (p = 0.037) and PD improved (p = 0.007) anxiety. Unemployment (p < 0.001) and low Hb (p = 0.018) worsened depression. PD improved (p = 0.002) and HD worsened (p < 0.001) emotional well-being. PD worsened social functioning (p = 0.002). PD (p = 0.007) and higher phosphate (p = 0.022) worsened and HD (p = 0.01) and higher Hb (p = 0.02) improved physical discomfort / pain.
Conclusion
Advanced chronic kidney disease increases anxiety and depression and limits quality of life. Peritoneal dialysis improves mental health and emotional wellbeing and preserves the ability to undertake economic activity but limits social functioning and causes greater physical discomfort. Targeting haemoglobin and phosphate may ameliorate modality effects on mental health and quality of life.
The postoperative prognosis of elderly intertrochanteric fracture patients as evaluated by the Chang reduction quality criteria
Authors: He Miao , Liu Jian , Deng Xu , Zhang xiaoxing 
Published: August 2022

Abstract
Objective
The aim of this study was to investigate the relationship between the Chang reduction quality criteria (CRQC) and the outcome of elderly intertrochanteric fractures based on a subgroup of follow-up time.
Methods
Retrospective analysis of 389 elderly patients with intertrochanteric fractures treated surgically from January 2019 to June 2021, including 130 males and 259 females; age 84.64 (77.50-89.74) years. Patient survival was followed by telephone between admission to hospital for fracture and death, or until study deadline (June 1, 2022). According to the CRQC, were divided into Poor, Acceptable, Excellent three groups. Based on total follow-up time and 1-year follow-up, univariate and multivariate Cox proportional hazard models were used to assess the association between CRQC and all-cause mortality in elderly intertrochanteric fractures. Further subgroup analysis was performed according to different clinical and biological characteristics to make the results more accurate.
Results
The mortality were 24.68% and 15.42% at 1 year and total follow-up, respectively. In the total follow-up time and 1-year follow-up time, the mortality of CRQC-Excellent was significantly lower than that of CRQC-Acceptable (p.adj < 0.05), and was significantly lower than that of CRQC-Poor (p.adj < 0.05). After multi-factor adjustment, CRQC-Acceptable and Poor in were independent risk factors affecting the overall mortality and 1-year mortality. In addition, advanced age, comorbidities ≥ 1, ASA3 + 4, and prolonged preoperative waiting time were independent risk factors for survival in the total follow-up time. At 1-year follow-up, only ASA3 + 4 and prolonged preoperative waiting time were independent risk factors for survival. Subgroup analysis according to different characteristics at the total follow-up time and at the one-year follow-up time showed that in most subgroups as CRQC decreased was significantly associated with an increase in all-cause mortality (p for trend < 0.05).
Conclusions
This study highlights that CRQC-Acceptable and Poor are associated with increased all-cause mortality in elderly intertrochanteric fractures. We should try to achieve good reduction of these fractures.
The efficacy and safety of topical saline-irrigation with tranexamic acid on perioperative blood loss in patients treated with percutaneous endoscopic interlaminar discectomy
Authors: yu zhang , Zhao Wenjie , Hu Man , Liu Xin , Peng Qing , Meng Bo , Yang Sheng , Feng Xinmin , Zhang Liang 
Published: August 2022

Abstract
Background: Adequate visualization in percutaneous endoscopic interlaminar discectomy (PEID) surgery is essential for the successful completion of lumbar discectomy. However, there is currently no relevant research on the efficacy and safety of adding tranexamic acid (TXA) to the saline irrigation fluid in PEID surgery. To evaluate the efficacy and safety of topical saline-irrigation with TXA for PEID in the treatment of lumbar disc herniation.Methods: Patients who underwent PEID surgery for L5-S1 lumbar disc herniation were divided into two groups. PEID was performed with saline-irrigation fluid containing 0.33 g of TXA per 1 L of saline in the TXA group (n=38) and only saline in the control group (n=51). All PEID surgeries were performed by the same spine surgery team. The hidden blood loss (HBL), intraoperative blood loss (IBL), total blood loss (TBL), amount of fluid used, operation time, visual clarity, hospital stay, blood transfusion rate, coagulation index and complication rate were compared between the two groups.Results: The TBL, HBL, and IBL in the TXA group were significantly lower than those of the control group. The postoperative hemoglobin in the TXA group was significantly higher than that of the control group. Visual clarity was significantly better and operation time was significantly shorter in the TXA group. However, there was no significant difference in postoperative hematocrit, blood coagulation function, amount of fluid used, blood transfusion rate and perioperative complications between the two groups.Conclusion: In PEID surgery, addition TXA to topical saline-irrigating fluid can significantly reduce HBL, IBL and TBL. Addition TXA to topical saline-irrigating fluid can improve visual clarity in the surgery and reduce operation time, but do not change coagulation function or increase complication rate.
CD206+ mono-macrophages are involved in Antineutrophil Cytoplasmic Antibodies associated glomerulonephritis
Authors: AN Xiao-ning , Wei Zhao-Nan , Xie Yin-Yin , Xu Jing , Shen Yan , Ni Li-Yan , Shi Hao , Shen Ping-Yan , Zhang Wen , Chen Yong-Xi 
Published: August 2022

Abstract
BackgroundAntineutrophil Cytoplasmic Antibodies (ANCA) associated glomerulonephritis (AGN) is a group of autoimmune diseases and mono-macrophages are involved in its glomerular injuries. We investigate role of CD206+ mono-macrophages in AGN to provide therapeutic targets for the disease. Methods 27 AGN patients (14 active AGN, 13 remissive AGN) together with healthy controls (n=9), disease controls (n=6) and kidney function adjusted controls (n=9) from Department of Nephrology, Ruijin hospital were recruited. Flow cytometry was used to study proportion of CD206+ cells in peripheral blood. Immunohistochemistry for CD206 staining was performed in kidney tissues. Serum soluble CD206 (sCD206) was measured by enzyme-linked immunosorbent assay (ELISA). We also generated murine myeloperoxidase (MPO) (muMPO) ANCA by immunizing Mpo-/- mice. Mouse bone marrow-derived macrophages (BMDMs) and peripheral blood mononuclear cell (PBMC) derived macrophages were treated with MPO ANCA with or without its inhibitor AZD5904 to investigate the effects of MPO-ANCA on CD206 expression.ResultsThe proportion of peripheral CD206+CD68+ cells in active AGN patients were significantly higher than that in remissive patients (p<0.001), healthy controls (p<0.001) and kidney function adjusted controls (p<0.001). Serum sCD206 level in active AGN patients was higher than that in healthy controls (p<0.05) and remissive patients (p<0.01). Immunohistochemistry showed CD206 was highly expressed in the kidney tissues in active AGN patients in comparison with disease controls. Further studies showed MPO ANCA could induce CD206 expression in BMDMs and PBMC derived macrophages and such effects could be reversed by its inhibitor AZD5904. Conclusion ANCA could induce CD206 expression on mono-macrophages and CD206+ mono-macrophages are activated in AGN. Serum sCD206 correlates to disease activity and it could serve as a potential therapeutic target for the disease.
EGFR-dependent aerotaxis is a common trait of breast tumour cells
Authors: MIKAELIAN Ivan , GADET Rudy , DEYGAS Mathieu , Bertolino Philippe , Hennino Anca , Gillet Germain , Rimokh Ruth , Berremila Sid-Ali , PEOC'H Michel , GONZALO Philippe 
Published: August 2022

Abstract
Background: Aerotaxis, the chemotactism to oxygen, is well documented in prokaryotes. We previously reported for the first time that non-tumorigenic breast epithelial cells also display unequivocal directional migration towards oxygen. This process is independent of the hypoxia-inducible factor (HIF)/prolyl hydroxylase domain (PHD) pathway but controlled by the redox regulation of epidermal growth factor receptor (EGFR), with a reactive oxygen species (ROS) gradient overlapping the oxygen gradient at low oxygen concentration. Since hypoxia is an acknowledged hallmark of cancers, we addressed the putative contribution of aerotaxis to cancer metastasis by studying the directed migration of cancer cells from their hypoxic environment towards nearby oxygen sources, modelling the in vivo migration of cancer cells towards blood capillaries.Methods: We subjected to the aerotactic test described in our previous papers cells isolated from fresh breast tumours analysed by the Pathology Department of the Saint-Etienne University Hospital (France) over a year. The main selection criterion, aside from patient consent, was the size of the tumour, which had to be large enough to perform the aerotactic tests without compromising routine diagnostic tests. Finally, we compared the aerotactic properties of these primary cells with those of commonly available breast cancer cell lines.Results: We show that cells freshly isolated from sixteen human breast tumour biopsies, representative of various histological characteristics and grades, are endowed with strong aerotactic properties similar to normal mammary epithelial cell lines. Strikingly, aerotaxis of these primary cancerous cells is also strongly dependent on both EGFR activation and ROS. In addition, we demonstrate that aerotaxis can trigger directional invasion of tumour cells within the extracellular matrix contrary to normal mammary epithelial cells. This contrasts with results obtained with breast cancer cell lines, in which aerotactic properties were either retained or impaired, and in some cases, even lost during the establishment of these cell lines.Conclusions:Altogether, our results support that aerotaxis may play an important role in breast tumour metastasis. In view of these findings, we discuss the prospects for combating metastatic spread. Trial registration: IRBN1462021/CHUSTE