bluuebunny/crossref_metadata_2025_split huggingface dataset:
A conundrum of r-protein stability: unbalanced stoichiometry of r-proteins during stationary phase in Escherichia coli
Authors: Reier Kaspar , Lahtvee Petri-Jaan , Liiv Aivar , Remme Jaanus 
Published: February 2022

ABSTRACTBacterial ribosomes are composed of three ribosomal RNA (rRNA) and over 50 ribosomal protein (r-protein) molecules. R-proteins are essential for ribosome assembly, structural stability, and also participate in almost all ribosome functions. Ribosomal components are present in stoichiometric amounts in the mature 70S ribosomes during the exponential and early stationary growth phases. Ribosomes are degraded in stationary phase, however, the stability and fate of r-proteins during stationary growth phase are not known. In this study, we report a quantitative analysis of ribosomal components during the extended stationary phase in E.coli. We show that: (a) the quantity of ribosomes per cell mass decreases in the stationary phase. (b) 70S ribosomes contain r-proteins in stoichiometric amounts. (c) 30S subunits are degraded faster than 50S. (d) In total proteome, the quantity of 21 r-proteins decreases during 14 days (short-lived r-proteins) concomitantly with the reduction of cellular RNA. (e) 30 r-proteins are stable and form a pool of free r-proteins (stable r-proteins). Thus r-proteins are present in non-stochiometric amounts in the proteome of E. coli during the extended stationary phase.IMPORTANCERibosome degradation has been extensively described from the viewpoint of its main component – rRNA. Here we aim to complement our knowledge by quantitatively analyzing r-protein degradation and stability in the ribosomes as well as in the whole-cell proteome during stationary phase in E. coli. R-proteins are considered to be very stable in the proteome. Here we show that a specific set of r-proteins are rapidly degraded after release from the rRNA. The degradation of r-proteins is an intriguing new aspect of r-protein metabolism in bacteria.
rG4-seq 2.0: enhanced transcriptome-wide RNA G-quadruplex structure sequencing for low RNA input samples
Authors: Zhao Jieyu , Chow Eugene Yui-Ching , Yeung Pui Yan , Zhang Qiangfeng Cliff , Chan Ting-Fung , Kwok Chun Kit 
Published: February 2022

ABSTRACTRNA G-quadruplexes (rG4s) are non-canonical structural motifs that have diverse functional and regulatory roles such as transcription termination, alternative splicing, mRNA localization and stabilization and translational process. We recently developed RNA G-quadruplex structure sequencing (rG4-seq) technique and discovered many rG4s in both eukaryotic and prokaryotic transcriptomes. However, rG4-seq suffers from complicated gel purification step and limited PCR product yield and thus requires a high RNA input amount, limiting its applications for physiologically or clinically relevant studies. In this study, we have developed rG4-seq 2.0 by introducing a new ssDNA adapter containing deoxyuridine in the library preparation to enhance the library quality with no gel purification step, less PCR amplification cycles and higher yield of PCR products. We demonstrate that rG4-seq 2.0 produced high quality cDNA libraries that supported reliable and reproducible rG4 identification at varying RNA inputs (as low as 10 ng amount of RNA). rG4-seq 2.0 also improved the rG4-seq calling outcome and nucleotide bias in rG4 detection persistent in rG4-seq 1.0. Our new method can improve the identification and study of rG4s in low abundance transcripts, and our findings can provide insights to optimize cDNA library preparation in other related methods.
Training induced improvements in knee extensor force accuracy are associated with reduced vastus lateralis motor unit firing variability
Authors: Ely Isabel A , Jones Eleanor J , Inns Thomas B , Dooley Síobhra , Miller Sarah B J , Stashuk Daniel W , Atherton Philip J , Phillips Bethan E , Piasecki Mathew 
Published: February 2022

AbstractBackgroundMuscle force output during sustained submaximal isometric contractions fluctuates around an average value and is known to be influenced by variation in motor unit (MU) firing rates. MU firing rate variability seemingly reduces following exercise training interventions, however, much less is known with respect to peripheral MU properties. We therefore investigated whether targeted force accuracy training could lead to improved muscle functional capacity and control, in addition to determining any alterations of individual MU features.MethodsTen healthy participants (7 females, 3 males, 27±6 years, 170±8 cm, 69±16kg) underwent a 4-week supervised, unilateral, force accuracy training intervention. The coefficient of variation for force (FORCECoV) and sinusoidal wave force tracking accuracy (FORCESinu) were determined at 25% maximal voluntary contraction (MVC) pre- and post-training. Intramuscular electromyography was utilised to record individual MU potentials from the vastus lateralis (VL) muscles at 25% MVC during sustained contractions, pre- and post-training.ResultsKnee extensor muscle strength remained unchanged following training, with no improvements in unilateral leg-balance. FORCECoV and FORCESinu significantly improved in only the trained knee extensors by ~13% (p=0.01) and ~30% (p<0.0001) respectively. MU firing rate variability significantly reduced in the trained VL by ~16% (n=8; p=0.001), with no further alterations to MU firing rate or neuromuscular junction transmission instability.ConclusionOur results suggest muscle force control and tracking accuracy is a trainable characteristic in the knee extensors, which is likely explained by the reduction in MU firing rate variability apparent in the trained limb only.
An employee-centered perspective on business processes: measuring “healthy business processes” and their relationships with people and performance outcomes
Authors: Reif Julia A.M. , Kugler Katharina G. , Stockkamp Mariella T. , Richter Selina S. , Benning Valerie M. , Muschaweck Lina A. , Brodbeck Felix C. 
Published: February 2022

PurposeTraditional approaches to business processes and their management consider the “people dimension” as an antecedent of process performance. The authors complemented this approach by considering employees as process perceivers and thus taking an employee-centered perspective on business processes. The authors investigated dimensions of healthy business processes, that is, processes which, while promoting performance, foster employee well-being.Design/methodology/approachBased on a qualitative dataset and two quantitative studies, the authors developed and validated a scale for healthy business processes, interpreted it from a salutogenic perspective and tested relationships with people and performance outcomes.FindingsThe scale comprises four factors reflecting the three dimensions of the salutogenic concept “sense of coherence”: manageability was represented by the factors process tools and process flexibility; comprehensibility was represented by the factor process description; and meaningfulness was represented by the factor management support. The scale and its subscales were significantly related to people and performance outcomes.Originality/valueThe authors propose that health-oriented business process management and performance-oriented business process management are two components of an integrated business process management that favors neither a functionalist, efficiency-oriented approach nor an employee-oriented approach, but takes both approaches and their interaction equally into account in the sense of person-process fit.
Adoption of open innovation in the COVID-19 emergency: developing a process-based information coordination system
Authors: Elia Gianluca , Margherita Alessandro , Massaro Alessandro , Vacca Angelo 
Published: February 2022

PurposeThe COVID-19 pandemic has stimulated a global movement of open innovation initiatives aimed to provide knowledge and tools to support policy decisions and actions in the emergency scenario. The authors describe an open innovation process aimed to build an information coordination system to reduce the infection diffusion within the population.Design/methodology/approachThe authors use coordination theory principles to elaborate a framework of activities, resources and dependencies among information resources and producers in the COVID-19 emergency. The framework was applied to develop a case study aimed at describing a health emergency system implemented by Dyrecta Lab (a research laboratory on computer science) and CITEL (a medical research center).FindingsThe authors describe the existence of relevant “flow,” “fit” and “share” dependencies within the activities of infection containment and medical treatment. The authors identify eight key resources and a number of actors involved in those activities, and describe a platform able to gather a multitude of epidemic-related metrics with the purpose to address dependencies and support decision making.Research limitations/implicationsThe authors provide insights for advancing the academic discussion on process coordination principles in time-constrained, volatile and highly demanding scenarios.Practical implicationsThe value of the authors’ research can be identified for practitioners engaged to develop innovative development projects for public utility. The authors provide a contribution also for first responders and health operators involved in management of the current and future emergencies.Originality/valueThe adoption of process coordination principles is a relatively new and powerful approach to analyze and optimize the processes that characterize the management of emergency scenarios. Besides, the study and application of open innovation in healthcare are partially limited.
In search of frugality in the Malaysian hotel industry: the role of green marketing strategies and government initiatives
Authors: Mohamed Sadom Nur Zulaikha , Quoquab Farzana , Mohammad Jihad 
Published: February 2022

PurposeThis study aims to shed light on the factors that affect frugality (FR) in the hotel industry. Specifically, it aims to test the role of environmental advertisement (EA) and eco-labelling (EL) on FR through green attitude (GA) in the Malaysian hotel industry. It also tested the role of government initiatives (GIS) as the moderator.Design/methodology/approachUsing the judgemental sampling technique, a total of 259 usable responses were gathered from hotel guests. Partial least squares structural equation modelling was used to test the study hypotheses.FindingsThis study found that EA and EL affect hotel guests’ GA positively. Additionally, the finding revealed that GA exerts a positive influence on FR. Furthermore, this study disclosed that GA mediates the relationship between green marketing strategies (EA and EL) and FR. Contrary to expectation, the moderating role of GIs was not supported in this study.Originality/valueThis is a pioneering study that investigates FR in the hotel industry. Further, this study developed new relationships such as the mediating role of GA between marketing strategies in terms of EA and EL and FR. In addition, the moderating effect of GIs on the relationship between GA and FR, which is comparatively new in the literature was developed. The findings from this study are expected to benefit the hoteliers, governments and the researchers that specialized in consumer behaviour study.
“I just want to word it better”: developing disciplinary literacies in an after-school spoken word poetry team
Authors: Lesus Melina , Vaughan Andrea 
Published: February 2022

PurposeThis study aims to explore how youth poets wrote in a community of practice and how their out-of-school poetry writing contributed toward developing disciplinary literacy.Design/methodology/approachIn this qualitative case study, the authors studied youth’s writing by drafting narrative field notes, collecting student writing and process drawings and interviewing participants.FindingsThe authors found that the poets in this study maintained ownership of their writing and engaged in writing processes in ways that reflected Behizadeh’s (2019) conception of authenticity as writing that connects both to students’ experiences, and to the purposes and audiences of their writing context.Practical implicationsThis out-of-school context provides implications for how English Language Arts teachers can rethink what disciplinary literacy looks like in classroom writing instruction.Originality/valueBy maintaining ownership of their writing, the youth agentively positioned themselves not only as students accumulating disciplinary knowledge but also as participants in a community of practice.
Green HRM: pathway towards environmental sustainability using AHP and FAHP in a nascent parsimony
Authors: Khatoon Aaisha , Khan Nawab Ali , Parvin Farhana , Wahid Mohammad Salman , Jamal Mohd Tariq , Azhar Saad 
Published: February 2022

PurposeThe objective of the work is to have an insight into the degree of the contemporary concept of greening the organization through human resource management (HRM) and to attempt to bridge the gap toward environmental awareness, as well as to observe the dimensions of green HRM (GHRM) practices and its impression toward environmental sustainability by using analytical hierarchy process (AHP) and FAHP.Design/methodology/approachThe research was carried out in two phases; multi-method was employed. The first phase mainly includes a systematic review to convene comprehensive knowledge of widespread GHRM practices to leverage environmental sustainability. In the second phase, the AHP and fuzzy AHP (FAHP) were employed to examine the influence of the respective dimension of GHRM practices toward environmental sustainability.FindingsThis study emphasizes the status of GHRM practices such as green recruitment and green selection, green performance management, green training and development, green compensation and rewards, green welfare aspects as a way to aid environmental issues. The findings suggest that these initiatives help to leverage environmental sustainability. Further, this paper reveals that green compensation and rewards have the highest impact on leveraging environmental sustainability. However, this study also emphasizes the comparative study of GHRM dimensions through AHP and FAHP.Originality/valueAs existing studies reveal, there is least research carried on this field of study and no study was conducted using AHP and FAHP in this field. Thus, this study reveals the necessity to discover the degree of concern toward GHRM practices in context to India.
Context-aware behaviour prediction for autonomous driving: a deep learning approach
Authors: R. Syama , C. Mala 
Published: February 2022

Purpose
This paper aims to predict the behaviour of the vehicles in a mixed driving scenario. This proposes a deep learning model to predict lane-changing scenarios in highways incorporating current and historical information and contextual features. The interactions among the vehicles are modelled using long-short-term memory (LSTM).

Design/methodology/approach
Predicting the surrounding vehicles' behaviour is crucial in any Advanced Driver Assistance Systems (ADAS). To make a decision, any prediction models available in the literature consider the present and previous observations of the surrounding vehicles. These existing models failed to consider the contextual features such as traffic density that also affect the behaviour of the vehicles. To forecast the appropriate driving behaviour, a better context-aware learning method should be able to consider a distinct goal for each situation is more significant. Considering this, a deep learning-based model is proposed to predict the lane changing behaviours using past and current information of the vehicle and contextual features. The interactions among vehicles are modeled using an LSTM encoder-decoder. The different lane-changing behaviours of the vehicles are predicted and validated with the benchmarked data set NGSIM and the open data set Level 5.

Findings
The lane change behaviour prediction in ADAS is gaining popularity as it is crucial for safe travel in a mixed driving environment. This paper shows the prediction of maneuvers with a prediction window of 5 s using NGSIM and Level 5 data sets. The proposed method gives a prediction accuracy of 97% on average for all lane-change maneuvers for both the data sets.

Originality/value
This research presents a strategy for predicting autonomous vehicle behaviour based on contextual features. The paper focuses on deep learning techniques to assist the ADAS.
Resilience of Brazilian health-care professionals during the pandemic
Authors: Araujo Claudia , Siqueira Marina , Amaral Liliana 
Published: February 2022

Purpose
Health-care professionals are caring for patients in unprecedented circumstances during the COVID-19 pandemic, dealing with scarce resources, higher demand and uncertain outcomes. In this context, the purpose of this study is to explore the views of health-care professionals regarding their work conditions and perceived impacts of the pandemic on their health, as well as the role of resilience and improvisation in face of the new challenges.

Design/methodology/approach
This exploratory and qualitative study carried out semi-structured interviews with eleven health-care professionals from three Brazilian states that have been working in intensive care settings during the pandemic.

Findings
The pandemic has posed a great personal and professional burden on the professionals, impacting their physical and mental health. It also has required them greater resilience and improvisation capabilities to adequately perform work-related activities.

Practical implications
In addition to individual-level attitudes, the results suggest that aspects in the government, society, personal relationships and providers domains influence the effects of the pandemic on the health-care professionals and how they cope with the ongoing crisis. Such a multifactorial approach should therefore be considered by health managers.

Originality/value
With no similar effort identified, this study emphasizes the relevance of discussing the pandemic burden on frontline professionals and intends to be useful for health practitioners, managers, academics and policymakers.
Workers' remittances in Central America: where does the money go?
Authors: Basnet Hem C. , Baker Josiah , Donou-Adonsou Ficawoyi 
Published: February 2022

PurposeThe purpose of this study is to examine two issues about remittances in Central American countries (Costa Rica, El Salvador, Guatemala, Honduras and Nicaragua). First, whether the inflow of remittances impacts income in the long run. Second, what motivates migrants to send remittances? The first issue is analyzed in the context of a permanent income hypothesis, while the second is analyzed in the context of altruism versus self-interest motives.Design/methodology/approachA panel cointegration method is used to establish the long-run relationship between the variables under consideration. Further, this study uses the fully modified ordinary least squares method (FMOLS) to estimate the impact of remittances on income and consumption. The pooled mean group (PMG) estimation is used.FindingsThe test results indicate that remittances into Central American countries do not promote growth in the long run. Central American families may perceive remittances as a permanent income stream and will increase their current consumption. Additionally, the test results indicate that sending remittances of the Central American migrants is mainly driven by altruism. Their primary motive is to support left-behind families at times of economic hardship.Research limitations/implicationsFindings provide an important implication for these Central American countries, as they have potential to boost income by utilizing remittance money in productivity-enhancing activities. This study could also provide valuable information for the governments of labor-exporting countries around the world to encourage and incentivize remittance recipient families to utilize those funds for income-generating activities.Originality/valueIn Central America, this is probably the first attempt in the literature to analyze the impact of remittances in the context of permanent income hypothesis and the motivation of Central American workers to send remittances to their countries of origin.
The impact of entrepreneurs’ decision-making style on SMEs’ financial performance
Authors: Weerasekara Sumedha , Bhanugopan Ramudu 
Published: February 2022

Purpose
This study aims to investigate the impact of entrepreneurs’ decision-making styles on enterprise performance and suggests several entrepreneurial ecosystems – factors are impacting this relationship. The authors extend this line of work by examining how regional entrepreneurial culture, educational institutional support and business and social networks mediating the relationship between entrepreneurs’ decision-making style and small medium enterprises (SME)s’ financial performance.

Design/methodology/approach
The data were collected through an e-survey of SME owners in New South Wales, Australia. This study developed a model combining a set of entrepreneurial ecosystem factors, entrepreneurs’ decision-making styles and SMEs’ financial performance. Data were analysed using partial least square structural equation modelling.

Findings
The results suggest regional entrepreneurial culture, educational institutional support and business and social networks mediate the relationship between entrepreneurs’ decision-making style and SMEs’ financial performance. Hence, this study developed a more complete methodical understanding of entrepreneurs’ decision-making styles and their impact on SMEs’ financial performance. This study provides deeper insights into the conditions and processes by which an entrepreneurs’ decision-making style impacts SMEs’ financial performance.

Originality/value
The focus of this study was to understand the relationship of entrepreneurs’ decision-making styles on SMEs’ financial performance. The authors identified that the entrepreneurs’ decision-making style positively impacts SMEs’ financial performance. This study augments the body of knowledge by proposing ways in how the entrepreneurs’ decision-making style can be more strengthened.