bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the mediation of DNA methylation across the epigenome between childhood adversity and First Episode of Psychosis – findings from the EU-GEI study
Authors: Alameda Luis , Liu Zhonghua , Sham Pak , Monica AAS , Giulia Trotta , Victoria Rodriguez , Forti Marta di , Simona Stilo , Radhika Kandaswamy , Arango Celso , Arrojo Manuel , Bernardo Miquel , Bobes Julio , de Haan Lieuwe , Del-Ben Cristina , Gayer-Anderson Charlotte , Lucia Sideli , Jones Peter , Jongsma Hannah , Kirkbride James , Cascia Caterina La , Lasalvia Antonio , Tosato Sarah , Llorca Pierre Michel , Menezes Paulo , Os Jim van , Diego Quattrone , Rutten Bart , Santos José , Sanjuan Julio , Selten Jean-Paul , Szöke Andrei , Tarricone Ilaria , Tortelli Andrea , Velthorst Eva , Morgan Craig , Dempster Emma , Hannon Eilis , Burrage Joe , Mill Jonathan , Murray Robin , Wong Chloe 
Published: November 2022

Abstract
Studies conducted in psychotic disorders have shown that DNA-methylation (DNAm) is sensitive to the impact of Childhood Adversity (CA). However, whether it mediates the association between CA and psychosis is yet to be explored. Epigenome-wide association studies (EWAS) using the Illumina Infinium-Methylation EPIC array in peripheral blood tissue from 366 First-episode of psychosis and 517 healthy controls was performed. Polyvictimization scores were created for abuse, neglect, and composite adversity with the Childhood Trauma Questionnaire (CTQ). Regressions examining (I) CTQ scores with psychosis; (II) with DNAm EWAS level and (III), then between DNAm and caseness, adjusted for a variety of confounders were conducted. Divide-Aggregate Composite-null Test for the composite null hypothesis of no mediation effect was conducted. Enrichment analyses were conducted with missMethyl package and the KEGG database. Our results show that CA was associated with psychosis (Composite: OR = 3.09; p = <0.001; abuse: OR = 2.95; p<0.001; neglect: OR = 3.25; p=<0.001). None of the CpG sites significantly mediated the adversity-psychosis association after Bonferroni correction (p<8.1x10-8). However, 28, 34 and 29 differentially methylated probes associated with 21, 27, 20 genes passed a less stringent discovery threshold (p<5x10-5) for composite, abuse, and neglect respectively, with a lack of overlap between abuse and neglect. These included genes previously associated with psychosis in EWAS studies, such as PANK1, SPEG TBKBP1, TSNARE1 or H2R. Downstream gene ontology analyses did not reveal any biological pathways that survived false discovery rate correction. Although at a non-significant level, DNAm changes in genes previously associated with schizophrenia in EWAS studies may mediate the CA-psychosis association. These results and associated involved processes such as mitochondrial or histaminergic dysfunction, immunity or neural signaling require replication in well-powered samples. Mediating genes were not associated with abuse and neglect, tentatively suggest differential biological trajectories linking CA subtypes and psychosis.
Affinity purification, identification and biochemical characterization of Gamma-Glutamyl Transpeptidase, a membrane anchored enzyme of Gigantocotyle explanatum
Authors: Farhat Faiza , Wasim Sobia , Rehman Lubna , Abidi S. M. A. 
Published: November 2022

Abstract
Gamma-glutamyl Transpeptidase is an enzyme that facilitates the transfer of glutamyl groups from glutamyl peptides to other peptides or water. Additionally, it also participates in important processes such amino acid transport, cellular redox control, drug detoxification, apoptosis, and DNA fragmentation in a various organism. In the present study, GGT activity in Gigantocotyle explanatum was examined in order to characterize the enzyme in the helminth system. GGT is isolated using membrane solubilization and purified through affinity column chromatography (Con-A Sepharose column). Km and Vmax values, as well as the optimal pH, optimal temperature, and incubation period, are also determined using enzyme kinetics. The hetero-dimeric property of the enzyme is demonstrated by the purified GGT, which yielded two subunits of 65.5 and 55 kDa. The optimal pH and temperature are found to be 8.0 and 37°C, respectively. While assessing the optimal incubation time of the enzyme, it was observed that the purified GGT not only retained its functional integrity up to 15 min but also reflected considerable thermostability at higher temperatures, by retaining 78% and 25% of its initial activities at 50°C and 60°C, respectively. 1 mM concentration of 6-Diazo-5-Oxo Nor-isoleucine (DON), a specific inhibitor of GGT, completely abolished GGT activity. These results suggest that GGT in these worms is a catalytically active enzyme with distinguishing characteristics that can be used for further study to comprehend its function in amphistome biology and in host-parasite relationships, especially since the potential therapeutic candidacy of the GGT enzyme has already been indicated in these groups of organisms.
Design of Edge Cloud Computing Recognition System for Intelligent Needs from the Perspective of Sports Psychology
Authors: Li Shuang , Liu Yu 
Published: November 2022

Abstract
This paper intends to meet society’s demand for intelligent recognition system design and improve the performance of the behavioral action recognition system. Based on the previous research, the behavior recognition system is optimized. The edge Cloud Computing (CLO) technology is introduced. Also, the idea of sports psychology is integrated. The overall scheme of the behavior recognition system is designed. The accuracy, precision, and recall of the recognition system in different behaviors are analyzed by training the data set. In addition, the cloud-based behavior recognition design model is compared with other algorithms to analyze the algorithm advantages. Finally, the training and validation tests are performed on the training set of expression images using the behavior recognition system mode. It is found that the behavior recognition system model can show high accuracy in different environments, all above 80%. The results show that the edge CLO recognition system that meets intelligence needs has good accuracy, precision, and recall in both action recognition and image expression recognition. The recognition effect is the best. This paper aims to provide some ideas for the research on intelligent behavior recognition and put forward some references for the field expansion of edge CLO technology applications. Besides, the intelligence level of the behavior recognition system is improved through software design.
A simplified model of tethered projectile systems wrapping around targets in space and a preliminary capture scheme based thereon
Authors: Ma Hongzhi , Lan Ding , Wang Tao , Wang Fude 
Published: November 2022

Abstract
The Euler-Eytelwein equation indicates that the tension from one end of the rope to the other end decays exponentially with the wrapping angle after wrapping around a target object, and the tensions at two ends can differ by several orders of magnitude. The ability to scale the force magnitude of this wrapping structure may be exploited for space captures. This paper simplifies the modeling of the actual scene of space capture, analyzes the kinematic characteristics of the wrapping process of tethered projectile systems, and obtains the trajectory equation of the projectile. Carrying out the force analysis of the element, forces acting on the satellite and the target object under different friction coefficients are obtained. Finally, considering two constraints of rope tension and wrapping time, as well as the number of wrapping turns for safe, a design scheme of projectile velocity and initial rope length required for successful capture is proposed.
Non-Autonomous Maximal Regularity Under Besov Regularity in Time
Authors: achache Mahdi 
Published: November 2022

Abstract
We consider the maximal regularity problem for non-autonomous Cauchy problems u′(t) + A(t)u(t) = f (t) t-a.e., u(0) = u0. Each operator A(t) arises from a time depending sesquilinear form a(t) on a Hilbert space H with constant domain V. We prove maximal Lp-regularity result for p ≤ 2 under min-imal regularity assumptions on the forms. Our main assumption is that (A(t))t∈[0,τ ] arepiecewise in the Besov space Bp1/2, 2 p with respect to the variable t. This improves previouslyknown results. We give three examples which illustrate our results.
Mathematics Subject Classification (2010): 35K90, 35K45, 47D06.
Multi-omics characterization of a scoring system to quantify hypoxia patterns in patients with head and neck squamous cell carcinoma
Authors: peng cong , Ye Huiping , Li Zhengyang , Yang Wen , Yi Zhuguang , Duan Xiaofeng 
Published: November 2022

Abstract
The 5-year survival rate of patients with head and neck squamous cell carcinoma (HNSCC) remains <50% due to risk of the recurrence and metastasis. Hypoxia patterns are a hallmark of HNSCC that are associated with its occurrence and progression. However, the precise role of hypoxia during HNSCC, such as the relationship between hypoxia, tumor immune landscape and cell communication orchestration remains largely unknown. The current study integrated data from bulk and single-cell RNA sequencing analyses to define the relationship between hypoxia and HNSCC. First, a scoring system named the hypoxia score (HS) was constructed based on hypoxia-related genes (HRGs) expression. The survival outcomes, stromal/immune/Estimate scores, responses to targeted inhibitors, and chemotherapeutic, and immunotherapy responses were distinct between a high HS group and a low HS group. Single-cell datasets showed different distributions of HS in immune cell populations. Furthermore, HLA-DPA1/CD4 axis was identified as a unique interaction between CD4+ T Conv and pDC cells. These findings may enhance the understanding of hypoxia characteristics associated with HNSCC.
Validity and Reliability of the Turkish Version of UPPS-P Impulsive Behavior Scale for Children (UPPS-P-C)
Authors: Tekeoğlu Uğur , Çöp Esra 
Published: November 2022

Abstract
Background
In this study we aimed to examine the Turkish validity and reliability of the UPPS-P Impulsive Behavior Scale for Children (UPPS-P-C) and to investigate whether there is a relationship between the UPPS-P-C subscales and Attention Deficit Hyperactivity Disorder (ADHD).
Method
The clinical sample of the study consisted of 50 children which are aged 10–14 with ADHD who applied to the Ankara City Hospital Child Psychiatry Clinic and did not receive treatment for at least 1 month. The community sample consisted of 525 children and adolescents. They were selected from the city of Sinop and their ages ranged from 10 to 14 years, all of whom were in middle school (5th to 8th grade). UPPS-P-C and Strengths and Difficulties Questionnaire (SDQ) – parent form were used in the study.
Results
Factor analysis showed that the five-factor model better explained the data. When the relationships between the subscales of the scale were examined, all relationships were found to be statistically significant. There was a lack of premeditation and lack of perseverance that had the highest ability to distinguish children with ADHD from the UPPS-P-C subscales. In the linear regression analysis, the model we tried to explain in the SDQ total and subscale scores with the UPPS-P-C subscale was found to be significant. The Cronbach α coefficient was found to be 0.894 for the UPPS-P-C scale. When the correlation between the total and subscales of the test and retest was examined, the Intra class Correlation Coefficient (ICC) was 0.782 and 0.771–0.897, respectively.
Discussion
Our findings show that the Turkish version of the UPPS-P-C has good validity and reliability and is successful in screening for features related to impulsivity. It has been shown that the UPPS-P-C scale can be used for symptom profiling and severity assessment rather than for solely diagnostic purposes.
On Feature Selection Algorithms for Effective Botnet Detection
Authors: Afroz Meher , Ibnath Muntaka , Rahman Ashikur , Sultana Jakia , Rab Raqeebir 
Published: November 2022

Abstract
The threats of botnets are becoming a growing concern infecting more and more computers every day. Although botnets can be detected from their behavioral patterns, it is becoming more challenging to differenti-ate the behavior between the malicious traffic and the legitimate trafficas with the advancement of the technologies the malicious traffics are fol-lowing the similar behavioral patterns of benign traffics. The detectionof malicious traffic largely depends on the traffic features that are beingused to feed in the detection process. Selecting the best features for effec-tive botnet detection is the main contribution of this paper. At the verybeginning, we show the impact of different features on botnet detectionprocess. Then we propose several heuristics to select the best featuresfrom a handful of possible features. Some proposed heuristics are trulyfeature-based and some are group-based, thus generating different accu-racy levels. We also analyze time complexity of each heuristic and providea detailed performance analysis. As working with all combinations of alarge number of features is not feasible, some heuristics work by groupingthe features based on their similarity in patterns and checking all combi-nations within the groups of small number of features which improves thetime complexity by a large margin. Through experiments we show the efficacy of the proposed feature selection heuristics. The result shows thatsome heuristics outperform state-of-the-art feature selection algorithms.
Mitochondrial lipid peroxidation and microsomal drug-metabolizing enzyme activity of rat hepatotoxicity under heavy metals from slag waste exposure
Authors: Mu Haishuo , Wan Wangjun , song Jingwu , Kuang Rong , Deng Tongle 
Published: November 2022

Abstract
Heavy metals from slag waste (HMSWs) have been a long-term concern around the world, posing serious environmental and visceral toxicity, especially hepatotoxicity. Investigating the leaching behavior and hepatotoxicity of HMSWs is of great significance. Based on the toxicogenomic analysis, the targets and related signaling pathways of heavy metal-induced liver injury were predicted, and the effects of different HMSWs on mitochondrial lipid peroxidation injury and microsomal drug-metabolizing enzyme activities in rats were experimentally explored. The results showed that different heavy metals might have the function of interfering with physiological processes such as oxidative stress, cell death, and energy metabolism regulation in vivo, and participate in the regulation of HIF-1 signaling pathway, peroxisomes, drug metabolism-cytochrome P450, ferroptosis, and other signaling pathways. HMSWs exposure caused weight loss, and significantly increased lactate dehydrogenase (LDH), malondialdehyde (MDA), alanine transaminase (ALT), and aspartate transaminase (AST) in different groups of rat liver, suggesting the presence of mitochondrial lipid peroxidation damage. Meanwhile, the activities of superoxide dismutase (SOD) and mitochondrial membrane potential (MMP) in the liver were significantly decreased, and microsomal Cytochrome P450 1A2 (CYP1A2) and 3A1 (CYP3A1) enzyme activities were induced, predicting the involvement of the microsomal drug-metabolizing enzymes. These results indicated that metabolic interplay between microsomes and other subcellular organelles including mitochondria and peroxisome could be involved in heavy metals-induced liver injury.
Factors impairing exclusive breastfeeding initiation in women who received labor neuraxial analgesia: A single-center retrospective observational study in Japan
Authors: Hirai Aki , Higuchi Misako , Wakabayashi Miho , Yagi Shigetaka , Minami Sawako , Ino Kazuhiko , Kawamata Tomoyuki 
Published: November 2022

Abstract
Background
Breastfeeding is beneficial for both the mother and infant. However, the factors impairing initiation of exclusive breastfeeding have not been sufficiently investigated in women that received labor neuraxial analgesia. This study aims to clarify the rate of successful initiation of exclusive breastfeeding in women that received labor neuraxial analgesia in our hospital, and any factors impairing it at one month postpartum.
Methods
We retrospectively reviewed the 376 women who had received labor neuraxial analgesia between January 2011 and May 2022. Univariate and multivariate logistic regression analyses were performed to determine factors influencing exclusive breastfeeding.
Results
The rate of exclusive breastfeeding at one month in women who received labor neuraxial analgesia was 43.1%. Exclusive breastfeeding was shown to have univariate association with multiparity (P = 0.0001), second stage of labor (P = 0.046), oxytocin use (P < 0.0001), vacuum extraction delivery (P = 0.001), amount of bleeding (P = 0.027), and neonatal birthweight (P = 0.010). Multivariate logistic regression analysis revealed that oxytocin use and vacuum extraction delivery were mother-related factors and lower birthweight was an infant-related factor that were significant predictors of impairment of the initiation of exclusive breastfeeding at one month after delivery.
Conclusion
Oxytocin use and vacuum extraction delivery as maternal factors and lower birthweight as an infant factor were shown to be a significant predictors of impairment of the initiation of exclusive breastfeeding at one month after delivery.
Echocardiographic assessment of left cardiac structure and function in ART-naïve people living with HIV/AIDS
Authors: Hu Xing , Zhang Yuan , Zhang Tong , Li Weihua , Han Jing , Zhang Xuhui , Meng Fankun 
Published: November 2022

Abstract
Background: Patients with human immunodeficiency virus (HIV) are at a significantly higher risk of cardiovascular disease (CVD) compared to HIV-negative people. CVD has become one of the leading causes of death from non-acquired immune deficiency syndrom (AIDS)-related events in people living with HIV/AIDS (PLWHA). Left heart dysfunction is the most common cardiac complication in PLWHA, and diastolic dysfunction is an important predictor of cardiovascular events. The aims of this study were 1) to detect changes in left cardiac structure and function in antiretroviral therapy (ART)-naive PLWHA using echocardiography; and 2) to investigate the risk factors for the development of left ventricular diastolic dysfunction (LVDD) in ART-naive PLWHA.
Methods: We retrospectively included 105 ART-naïve PLWHA and included 90 healthy subjects as controls to compare the differences in left heart structure and function between the two groups. Univariate and multifactorial logistic regression were employed to explore the risk factors of the development of LVDD in ART-naive PLWHA.
Results: The left ventricular end-diastolic internal diameter (LVEDD), left ventricular mass index (LVMI), and left atrial volume index (LAVI) were significantly greater in PLWHA than in controls (p＜0.05). The E/A ratio, lateral e' velocity, and mitral deceleration time (DT) were significantly lower in PLWHA than in controls (p < 0.05). Average E/e' ratio was significantly higher in PLWHA than in controls (p < 0.05). Left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) were not significantly different between PLWHA and controls (p＞0.05). Multifactorial logistic regression analysis showed that age, body mass index (BMI), and CD4+ count < 200 cells/μL were independent influencing factors for LVDD in ART-naive PLWHA (OR=1.781, 1.228, 3.683, p＜0.05).
Conclusions: Left ventricular systolic function did not differ between PLWHA and controls, and left ventricular diastolic function was lower in PLWHA than in controls. Age, BMI, and CD4+ count were independent factors affecting LVDD in ART-naive PLWHA.
Trial registration: ChiCTR1900021008，2019/01/24
Transcriptome analysis reveals vimentin-induced downregulation of cell-cell associations augments cancer cell migration
Authors: Usman Saima , Jamal Ahmad , Bushaala Antesar , Waseem Naushin H , Al-Dehlawi Hebah , Yeudall Andrew , Teh Muy-Teck , Tummala Hemanth , Waseem Ahmad 
Published: November 2022

Abstract

Background Vimentin is a type III intermediate filament (IF) protein, whose expression correlates with advanced metastatic cancer, reduced patient survival and poor prognosis across many cancers. During EMT-induced metastasis when vimentin begins to express, the epithelial characteristics are lost, and cell motility is augmented. The molecular bases for these changes are not well defined. Methods Ectopic expression of vimentin was carried in MCF-7 using spinfection of retroviruses. shRNA was used to knockdown vimentin in vimentin overexpressing MCF-7 and MDM-MB-231 cells, which express vimentin endogenously. The transcriptome profiling was carried out by RNA-Seq and validated by qPCR. Protein expression was measured by western blotting. Effect of vimentin on MCF-7 was determined by cell proliferation, migration and adhesion assays. Results Vimentin expression elicited a change in cell shape by significantly decreasing major axis, major axis angle and increasing cell migration, with no change in cell proliferation. Vimentin suppresses expression of major keratin genesKRT18,KRT19andKRT8. Transcriptome-coupled GO and KEGG analyses revealed that vimentin-affected genes were linked to either cell-cell/cell-ECM or cell cycle/proliferation specific pathways. Using shRNA mediated downregulation of vimentin in two cell types; MCF-7FV (ectopically expressing vimentin) and MDA-MB-231 (endogenously expressing vimentin), we identified 13 vimentin-responsive protein encoding genes common in both approaches and two long non-coding RNAs,LINC00052andC15ORF9-AS1. Eight of these gene products CDH5, AXL, PTPRM, TGFBI, CDH10, FOXM1, BCL2 and NES were associated with cell-cell and cell-ECM interactions, E2F1, FOXM1 and CDC45 were in the cell proliferation group and the rest FSD1, BCL2, KIF26A and WISP2 were outside the two groups. Interestingly, downregulation of CDH5 significantly increased MCF-7 cell migration. Furthermore, vimentin expression in MCF-7 reduced nuclear area, altered expression of lamins, which was mostly reversed after its downregulation. Conclusion Collectively, we demonstrate, for the first time, that vimentin expression in cancer cells downregulates genes maintaining cell-cell junctions resulting in increased cell migration. Furthermore, this is the first report linking vimentin expression with LINC00052, which is dysregulated in many cancers.