bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSII-3 Effect of Protein Supplementation Level and Degradability on Forage Utilization by Steers Consuming Low-Quality Forage: II. Bos taurus indicus
Authors: Cox Jodi R , Koy Kali K , Wickersham Tryon A 
Published: October 2022

Abstract
A deficiency of ruminally available N (RAN) caused by a basal diet of low-quality forage may depress forage utilization. Supplemental protein improves utilization by addressing the RAN therefore increasing intake and potentially improving digestibility. The objective of this study was to determine the effect of ruminal degradability and level of protein supplementation on forage utilization by Bos taurus indicus steers. Accordingly, five ruminally cannulated Brahman steers (BW 369 ± 51 kg) were used in a 5 × 5 Latin square to evaluate the interaction between level and degradability of protein supplementation when consuming a basal diet of King Ranch Bluestem hay (3.5% CP, 71.0% NDF). Treatments were arranged as a 2 × 2 factorial with steers receiving protein supplements (43% CP) having two levels of protein degradability (35 or 70% RDP) fed at two levels of supplementation, 1.26 or 2.53 g/kg BW (LOW and HIGH). A control treatment providing no supplemental protein was also included (CON). Five 14-d periods were conducted, consisting of 9 d adaptation, 4 d to measure intake and digestion, and 1 d for ruminal fermentation. No degradability × level interactions were observed for any measures of intake or digestion (P &gt; 0.10). Protein supplementation linearly increased (P &lt; 0.01) total digestible OM intake (TDOMI), NDF intake (P = 0.01), and total digestible NDF intake (DNDFI; P &lt; 0.03). Total digestible OMI was greater in steers receiving 70% RDP than the 35% RDP (P = 0.08). This response resulted from the linear increase in FOMI with 75% RDP (P = 0.03), that was not observed with 35% RDP. Despite differences in FOMI, TDOMI increased linearly (P ≤ 0.03) for both 35 and 70% RDP, partially the result of providing a highly digestible supplement. Forage OMI differences also resulted in NDFI being greater for 70% RDP than 35% RDP (P = 0.06) and DNDFI increasing linearly with the addition of both supplements (P ≤ 0.03). Organic matter digestibility (OMD) increased linearly with supplementation as a result of supplement provision. In contrast to observations previously reported in Bos taurus steers, provision of 35% RDP supplement did not improve FOMI. Supplementation with 70% RDP improved FOMI 19 and 20% compared to the basal diet at LOW and HIGH levels, respectively. A portion of this relatively modest response to protein supplementation could be attributed to greater intake in the unsupplemented controls.
PSIII-3 Late-Breaking: Diisobutyl Phthalate (DIBP) at Low Doses Alters Gene Expression of Epigenetic Regulators in Bovine Granulosa Cells
Authors: Pena Bello Camilo P , Batalha Isadora M , Archilia Evandro C , Wulstein Katarina , Schütz Luis F F 
Published: October 2022

Abstract
Phthalates are ubiquitous environmental contaminants and impact reproduction and epigenetic mechanisms. The use of diisobutyl phthalate (DIBP) has increased in the last decade. However, studies about DIBP's effects on female reproductive physiology are limited. Methylation of DNA and RNA are epigenetic processes that regulate gene expression, cell biology, and reproductive functions. How DIBP exposure regulates these epigenetic mechanisms in ovarian follicular cells remains to be unveiled. We hypothesize that DIBP at low doses alters the expression of RNA and DNA methylation regulators in bovine granulosa cells (GC). Bovine GC was isolated from small antral follicles (1-5 mm on surface diameter) from ovaries obtained at a slaughterhouse. Cells were cultured in vitro until reaching an average 70% confluence and treated with DIBP at levels detected in human blood (0 ng/mL, 1 ng/mL, 10 ng/mL, 100 ng/mL) combined with hormones that regulate ovarian folliculogenesis: FSH or FSH+IGF1. Cells were cultured for 24h for total RNA isolation (n = 5 pools) or for 48h for cell counting (n=5 pools). Relative mRNA abundance of RNA N6 methyladenine (m6A) methylation machinery (m6A writers: METTL3, METTL14; m6A erasers: ALKBH5, FTO), RNA 5-methylcytosine (m5C) methylation regulators (NSUN2, TRDMT1), and DNA methylation players (DNA methyltransferases: DNMT1, DNMT3A, DNMT3B; DNA demethylases: TET1, TET2, TET3) was determined via qPCR using 18S as housekeeping gene and expressed as 2-ΔΔCt. Cell proliferation was analyzed by cell counting using a MUSE cell analyzer. Data were analyzed via one-way ANOVA with GLM procedures of SAS for Windows. Normality was tested using Shapiro-Wilk test procedures and data considered not normal were transformed to log2 (Y + 0.6). Data sets that showed non-gaussian distribution after log-transformation was analyzed via the Kruskal-Wallis test using GraphPad Prism 9.2.0. Cell proliferation was not affected by DIBP treatments in combination with FSH or with FSH+IGF1. In GC treated with FSH in combination with DIBP, there was no significant effect on relative mRNA abundance of RNA or DNA methylation players. In combination with FSH+IGF1, GC treated with DIBP had lower (P &lt; 0.05) METTL14 relative mRNA abundance at 1 ng/mL and 10 ng/mL than at 0 ng/mL and 100 ng/mL. TRDMT1 relative mRNA abundance was lower (P &lt; 0.05) in the DIBP group at 10 ng/mL in comparison to groups 0 ng/mL and 1 ng/mL, but no 100 ng/mL. In addition, there was no significant effect on the relative mRNA abundance of DNA methylation regulators. In summary, GC treated with FSH+IGF1 and DIBP at low doses alters the gene expression of RNA methylation players in m6A and m5C RNA modifications in a non-monotonic dose-response, but does not alter mRNA abundance of DNA methylation regulators.
PSIII-5 Late-Breaking: Genetic Evaluation of Energy Efficiency in Bos taurus Cows Classified by Residual Feed Intake
Authors: Schaff Nathan , Dafoe Julia , Boss Darrin L , Thomson Jennifer M , Boles Jane A 
Published: October 2022

Abstract
Genetic selection for efficiency traits is essential to improve the profitability and sustainability of livestock operations. Inefficient metabolism and energy production in animal physiology leads to excess feed consumption, resulting in higher feed costs. The objective of this study was to investigate hepatic gene expression in mature Angus-influenced cows that were classified as low or high RFI as heifers and had either a low or high ratio of calf weaning weight to mature body weight at weaning. Eleven cows were selected from the Northern Agricultural Research Center (NARC) research herd. Cows were ranked as efficient (n = 5) or inefficient (n = 6) based on their low or high residual feed intake (RFI) measured as a growing heifer and then by the ratio of calf weaning weight by mature body weight. Animals were partitioned from the bottom quartile with low RFI as a heifer and weaned with a higher proportion of the calf were classified as efficient, and animals from the top quartile with a high RFI as a heifer and a lower ratio of the calf to body weight were classified as inefficient. Liver samples were collected by needle biopsy approximately one week after weaning and snap frozen. The liver gene expression profile was measured using RNAseq. Two cows' data were not reported, one from each group, as RNA quality prevented sequencing (Efficient n = 4 and Inefficient n = 5). Over 5 million reads were mapped for each sample and were aligned to BTau 4.6.1 reference sequence using HISAT2. Differentially expressed genes were determined using the DEseq2 R package using a model based on the negative binomial distribution, and p-values were adjusted using the Benjamini-Hochberg approach. A corrected P-value of less than 0.05 defined a differentially expressed gene. Gene lists were used for gene ontology and KEGG pathway enrichment using clusterProfiler software. One novel gene was significantly differentially expressed with higher expression in the efficient group. However, no annotation currently exists for this gene. Additionally, the KEGG pathway biosynthesis of unsaturated fatty acids was significantly enriched (P &lt; 0.05) with the following differentially expressed genes SCD, FADS1/2, ELOVL6, and HACD2, all being down-regulated in inefficient cows. The enrichment of this pathway is most functionally relevant, as it indicates that local liver changes could directly affect the efficient metabolism and biosynthesis of fatty acids. With encouraging, but limited results, this study provides the groundwork for future study of the heritable mechanisms contributing to the generation of more energy-efficient cows.
A novel bio-inspired approach with multi-resolution mapping for the path planning of multi-robot system in complex environments
Authors: Yi Xin , Zhu Anmin , Li Chaofan , Yang Simon X 
Published: October 2022

Abstract
For multi-robot systems (MRSs), conventional path planning with single resolution mapping is challenging to balance information and computation. Regarding path planning of MRS, the previous research lacked systematic definition, quantitative evaluation, and the consideration of complex environmental factors. In this paper, a new systematic formulation is proposed to redefine the multi-robot path planning problem in complex environments, and evaluate the related solutions of this problem. To solve this problem, a novel bio-inspired approach based on reaction-diffusion system is given to deal with the path planning of MRS in complex environments, such as electromagnetic interference, ocean currents, and so on. Furthermore, a multi-layer neural dynamic network is proposed to describe environments with multiple resolutions, which can improve time performance while ensuring the integrity of environmental information. Comparative experimental results indicate that the proposed approach shows the excellent path planning performance of MRS in complex environments. The stability of the proposed method is determined by the mathematical basis.
Public Versus Secret Voting in Committees
Authors: Mattozzi Andrea , Nakaguma Marcos Y 
Published: October 2022

Abstract
We study the effect of transparency of individual votes in committees where members are heterogeneous in competence and bias, they are career-concerned, and they can abstain. We show that public voting attenuates the biases of competent members and secret voting attenuates the biases of incompetent members. Public voting leads to better decisions when the magnitude of the bias is large, while secret voting performs better otherwise. We present novel experimental evidence consistent with our theory.
Patterns and Ecological Mechanisms of Tick-Borne Disease Exposure Risk in Acadia National Park, Mount Desert Island, Maine, United States
Authors: McBride Sara E , Lieberthal Brandon A , Buttke Danielle E , Cronk Brittany D , De Urioste-Stone Sandra M , Goodman Laura B , Guarnieri Lucy D , Rounsville Thomas F , Gardner Allison M 
Published: October 2022

Abstract
National parks are unique and significant vector-borne pathogen transmission settings, engaging over 300 million people in outdoor recreation per year. In this study, we integrated vector surveys and ecological habitat feature data in spatial models to characterize tick-borne disease exposure risk in Acadia National Park (ANP), Maine. To determine the broad-scale patterns of blacklegged tick Ixodes scapularis Say (Acari: Ixodidae) densities in ANP, we conducted host-seeking tick collections at 114 sites across the park over two years. Using these tick survey data and geospatial landscape feature data (i.e., land cover, elevation, forest patch size, and aspect) we developed a random forest model of nymphal tick density. We found that host-seeking tick density varies significantly across the park and is particularly high in areas characterized by deciduous forest cover and relatively low elevation. To explore potential fine-scale ecological drivers of tick density spatial patterns, we quantified microclimate conditions, host activity, and vegetation characteristics at a subset of 19 sites. We identified significant differences in microclimate conditions but not host activity or vegetation metrics across broad-scale landscape feature classes. Mean temperature and mean humidity were correlated to nymphal densities and therefore may provide a mechanistic link between landscape features and blacklegged tick densities. Finally, we detected multiple tick-borne pathogens in both ticks and small mammals sampled in ANP, including Borrelia burgdorferi, Babesia microti, and Anaplasma phagocytophilum. Our findings demonstrate the value of using ecological metrics to estimate vector-borne disease exposure risk and provide insight into habitat characteristics that may drive tick-borne disease exposure risk.
Populist attitudes and politicians’ disinformation accusations: effects on perceptions of media and politicians
Authors: Egelhofer Jana Laura , Boyer Ming , Lecheler Sophie , Aaldering Loes 
Published: October 2022

Abstract
Populist politicians increasingly accuse opposing media of spreading disinformation or “fake news.” However, empirical research on the effects of these accusations is scarce. This survey experiment (N = 1,330) shows that disinformation accusations reduce audience members’ trust in the accused news outlet and perceived accuracy of the news message, while trust in the accusing politician is largely unaffected. However, only individuals with strong populist attitudes generalize disinformation accusations to the media as an institution and reduce their general media trust. The phrase “fake news” does not amplify any of these effects. These findings suggest that politicians can undermine the credibility of journalism without much repercussion—a mechanism that might also threaten other authoritative information sources in democracies such as scientists and health authorities.
Discovery and study of abrasion behavior in granular shear flow
Authors: Hsiau Shu-San , Hutter Kolumban , Lu Li-Shin , Teufel Andreas , Chou Shih-Hao , Sheng Li-Tsung 
Published: October 2022

ABSTRACT
Experiments were performed in an Couette ring shear cell with particles from the food industry of nearly spherical geometry and nominal diameters between 2.0 and 6.7 mm and with quartz grains with 2.1 to 3.3 mm diameter to examine whether rapid shear flow would give rise to an appreciable change in the granular properties. In particular, it was suspected that surface roughness would change during shearing and lead to changes in the values of the internal and wall friction angles. Measuring these angles corroborated the supposition that abrasion of the particles reduced their frictional resistance, both via the internal and wall friction angles. This demonstrates that roughness changes may occur in granular flows and should in those situations not be ignored in the theoretical descriptions.
Dissatisfied, but not discouraged: the effects of French female lawyers perceiving gender discrimination
Authors: Insarauto Valeria , Boni-Le Goff Isabel , Mallard Grégoire , Lépinard Eléonore , Le Feuvre Nicky 
Published: October 2022

AbstractPrevious research has suggested not only that gender discrimination is widespread in law firms, conditioning women’s career paths and full integration into the legal profession, but also that female lawyers are more likely than their male counterparts to perceive unfair treatment. However, little research exists on how female lawyers’ perceptions of gender discrimination may affect their individual work experiences, in particular their attitudes toward their job and their career. This article aims to fill this gap by examining how perceived gender discrimination affects female lawyers’ job satisfaction with their career prospects and work–life balance, as well as their intentions to leave the legal profession. With a focus on the under-researched French case, it draws on the quantitative analysis of the data collected from an online survey administered to 663 female Parisian lawyers. Results show that perceptions of discrimination negatively affect women’s satisfaction with regard to their career prospects and work–life balance, but do not have any influence on their quitting intentions. By adopting a ‘view from below’ on the individual work experiences of female lawyers, the article sheds new light on the dynamics of women’s disadvantage in legal careers.
Immunogenicity of a single fractional intradermal dose of Japanese encephalitis live attenuated chimeric vaccine
Authors: Furuya-Kanamori Luis , Gyawali Narayan , Mills Deborah J , Mills Christine , Hugo Leon E , Devine Gregor J , Lau Colleen L 
Published: October 2022

AbstractBackgroundJapanese encephalitis (JE) is endemic in Asia and the western Pacific. Vaccination is recommended for travellers to endemic regions, but the high cost of the vaccine is a major barrier to uptake.MethodsA quasi-experimental, pre-post intervention clinical trial without a control group was conducted to assess the immunogenicity and safety of intradermal (ID) JE vaccine. Healthy adults (18–45 years) received one dose of 0.1 mL (20% of standard dose) ID Imojev® (JE live attenuated chimeric vaccine, Sanofi-Aventis). Adverse events following immunization (AEFIs) were recorded 10 days post-vaccination. Blood samples were collected at baseline, 4 and 8 weeks post-vaccination. Neutralizing antibodies were measured using 50% plaque reduction neutralization test (PRNT50). Seroconversion was defined as PRNT50 titre ≥10. An in vitro study was also conducted to quantify the rate of decay of vaccine potency after reconstitution.ResultsIn total, 51 participants (72.6% females, median age 31 years), all non-reactive to JE virus at baseline were enrolled. Mild and moderate AEFIs were reported by 19.6% of participants; none required medical attention or interfered with normal daily activities. All participants seroconverted at 4 weeks (GMT 249.3; 95%CI:192.8–322.5) and remained seropositive at 8 weeks (GMT 135.5; 95%CI:104.5–175.6). Vaccine potency declined at a rate of 0.14 log plaque-forming units/0.5 mL per hour.ConclusionsIn healthy adults, a single 0.1 mL ID dose of Imojev was safe and immunogenic, at least in the short term. Reconstituted vials of Imojev vaccine may not retain their potency after 6 hours. Fractional JE ID vaccination could be a cheaper yet effective alternative for short-term travellers. Further studies need to investigate the immune response in a wider age range of individuals and the long-term immunogenicity of fractional JE ID vaccines.Clinical trials registrationACTRN12621000024842.
Exposure to past immigration waves and attitudes toward newcomers
Authors: Gihleb Rania , Giuntella Osea , Stella Luca 
Published: October 2022

AbstractHow does previous exposure to massive immigrant inflows affect concerns about current immigration and the integration of refugees? To answer this question, we investigate attitudes toward newcomers among natives and previous immigrants. In areas that in the 1990s received higher inflows of immigrants of German origin—the so-called ethnic Germans—native Germans are more likely to believe that refugees are a resource for the economy and the culture, viewing them as an opportunity rather than a risk. Refugees living in these areas report better health and feel less exposed to xenophobia.
Leptocline as a shallow substructure of near-surface shear layer in 3D radiative hydrodynamic simulations
Authors: Kitiashvili Irina N , Kosovichev A G , Wray A A , Sadykov V M , Guerrero G 
Published: October 2022

ABSTRACT
Understanding the effects driven by rotation in the solar convection zone is essential for many problems related to solar activity, such as the formation of differential rotation, meridional circulation, and others. We analyse realistic 3D radiative hydrodynamics simulations of solar subsurface dynamics in the presence of rotation in a local domain 80 Mm wide and 25 Mm deep, located at 30° latitude. The simulation results reveal the development of a shallow 10 Mm deep substructure of the near-surface shear layer (NSSL), characterized by a strong radial rotational gradient and self-organized meridional flows. This shallow layer (‘leptocline’) is located in the hydrogen ionization zone associated with enhanced anisotropic overshooting-type flows into a less unstable layer between the H and He ii ionization zones. We discuss current observational evidence of the presence of the leptocline and show that the radial variations of the differential rotation and meridional flow profiles obtained from the simulations in this layer qualitatively agree with helioseismic observations.
Stellar feedback impact on the ionized gas kinematics in the dwarf galaxy Sextans A
Authors: Gerasimov Ivan S , Egorov Oleg V , Lozinskaya Tatiana A , Moiseev Alexei V , Oparin Dmitry V 
Published: October 2022

ABSTRACT
Feedback from massive stars shapes the interstellar medium (ISM) and affects the evolution of galaxies, but its mechanisms acting at the small scales (∼10 pc) are still not well constrained observationally, especially in the low-metallicity environments. We present the analysis of the ionized gas (focusing on its kinematics, which were never studied before) and its connection to the massive stars in the nearby (D ∼ 1.4 Mpc) star-forming very metal poor ($Z\sim 0.07 \, \mathrm{Z}_\odot$) galaxy Sextans A. The analysis is based on the observations with a scanning Fabry–Perot interferometer, long-slit spectroscopy, and imaging in emission lines with narrow-band tunable filters. We found 10 expanding superbubbles of ionized gas with ages of 1–3 Myr. We argue that three of them are probable supernova remnants, while the pre-supernova feedback is an important source of energy for blowing out the remaining superbubbles. The two brightest sites of star formation exhibit signs of outflowing ionized gas, which is traced by its ionized and atomic gas kinematics and (in one case) by its emission line flux ratios. Overall, the ionized gas kinematics in Sextans A is highly affected by the feedback from several generations of massive stars and inconsistent with the mere solid-body rotation observed in atomic hydrogen.
The density distribution and physical origins of intermittency in supersonic, highly magnetized turbulence with diverse modes of driving
Authors: Beattie James R , Mocz Philip , Federrath Christoph , Klessen Ralf S 
Published: October 2022

ABSTRACT
The probability density function (PDF) of the logarithmic density contrast, s = ln (ρ/ρ0), with gas density ρ and mean density ρ0, for hydrodynamical supersonic turbulence is well known to have significant non-Gaussian (intermittent) features that monotonically increase with the turbulent Mach number, $\mathcal {M}$. By studying the mass- and volume-weighted s-PDF for an ensemble of 36 sub-to-trans-Alfv́enic mean-field, supersonic, isothermal turbulence simulations with different modes of driving, relevant to molecular gas in the cool interstellar medium, we show that a more intricate picture emerges for the non-Gaussian nature of s. Using four independent measures of the non-Gaussian components, we find hydrodynamical-like structure in the highly magnetized plasma for $\mathcal {M} \lesssim 4$. However, for $\mathcal {M} \gtrsim 4$, the non-Gaussian signatures disappear, leaving approximately Gaussian s-statistics – exactly the opposite of hydrodynamical turbulence in the high-$\mathcal {M}$ limit. We also find that the non-Gaussian components of the PDF increase monotonically with more compressive driving modes. To understand the $\mathcal {M} \lesssim 4$ non-Gaussian features, we use one-dimensional pencil beams to explore the dynamics along and across the large-scale magnetic field, $\mathrm{{\boldsymbol {\mathit {B}}}}_0$. We discuss kinetic, density, and magnetic field fluctuations from the pencil beams, and identify physical sources of non-Gaussian components to the PDF as single, strong shocks coupled to fast magnetosonic compressions that form along $\mathrm{{\boldsymbol {\mathit {B}}}}_0$. We discuss the Gaussianization of the $\mathcal {M} \gtrsim 4$s-fields through the lens of two phenomenologies: the self-similarity of the s-field and homogenization of the dynamical time-scales between the over- and underdense regions in the compressible gas.