bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression pattern of FmNAC11 transcription factor in Fraxinus mandshurica and its function in improving cold resistance
Authors: Cao Yang , Song Fei , Liu Zhang , Zhan Yaguang 
Published: September 2022

Abstract
Background: NAC domain proteins contain one of the largest families of plant specific TFs. NAC (Nam, no apical meristem; ataf1/2, Arabidopsis transcription activation factor; CUC2, cup-shaped cotyledon) is an acronym for the names of three genes originally described as containing domains, namely Nam (no apical meristem), ataf1/2 (Arabidopsis transcription activator) and CUC2 (cupular cotyledon) are plant specific transcription factors. As a regulator of gene expression, transcription factors (TF) play an important role in plant development and response to hormone signals. In order to clarify the function of Fraxinus mandshurica fmnac11 gene under biological and abiotic stress,
Results: the cloned fmnac11 gene of Fraxinus mandshurica was 891bp, encoding 296 amino acids. Subcellular localization showed that fmnac11 was located in the nucleus and was indeed a transcription factor with transcriptional activation activity. Fmnac11 can respond to cold, NaCl stress, and IAA, GA, ABA hormone signal induction. The promoter length of the cloned fmnac11 gene was 2340bp. By analyzing cis acting elements, it was found that the promoter of fmnac11 gene responded to biological and abiotic stresses, participated in a variety of biological pathways, and regulated biological processes. Four transgenic lines overexpressing fmnac11 were obtained by Agrobacterium mediated transformation of WT tissue culture seedlings of Benxi tobacco. The transgenic plants grew rapidly after transplantation- Under the condition of low temperature culture at -5 ℃ for 24 hours, the wilting and yellowing degree of transgenic plants is significantly less than that of ordinary tobacco, and the transgenic tobacco overexpressing fmnac11 performs better in membrane system, osmotic system and ROS scavenging system than ordinary tobacco, indicating that fmnac11 gene has the function of improving plant cold resistance.
Conclusion: the gene expression of transgenic tobacco under cold treatment shows that fmnac11 transcription factor has a regulatory relationship with growth related IAA and AUX1 genes, cold resistance related ice, DREB and CBF1, ROS clearance related cat and SOD genes, which promotes the rapid growth, cold resistance and ROS clearance ability of transgenic tobacco.
Optimized construction of marble pieces as a thermal storage materials for performance improvement of hemispherical solar distiller
Authors: Attia Mohammed El Hadi , Kabeel Abd elnaby , Abdelgaied Mohamed , Sathyamurthy Ravishankar 
Published: September 2022

Abstract
The present work aims to obtain the optimized construction of marble pieces that achieves the highest hemispherical solar distiller’s performance. To achieve this idea, four different marble pieces (black marble pieces, green marble pieces, red marble pieces, and white marble pieces) were tested to obtain the optimal construction of the marble that achieves the highest hemispherical solar distiller performance. The marble pieces were placed on a basin of hemispherical distillers which represent absorber materials, as well as the sensible thermal storage materials. This was done by design and constructed three hemispherical distillers, first represent the classical hemispherical distiller (CHD) which represent the reference case, but the marble pieces were placed inside the basin of the second and third distiller. The experimentation tested were conducted on two cases; in the first case we tested two types of marble pieces (black and green marble pieces) by placed them in the second and third distiller (hemispherical distiller with black marble pieces (HSD-BMP) and hemispherical distiller with green marble pieces (HSD-GMP)). In the second case we tested the other two types of marble pieces (red and white marble pieces) by placed them in the second and third distiller (hemispherical distiller with red marble pieces (HSD-RMP) and hemispherical distiller with white marble pieces (HSD-WMP)). The results showed that utilization of red marble pieces (HSD-RMP) represents a good choice which improved the accumulative productivity to 7.75 l/m2 day compared to 4.8 l/m2day that achieved by the CHD with an improvement of 61.46%. The accumulative thermal efficiency of CHD reached 39.25%, while the utilization of red marble pieces (HSD-RMP) improved the accumulative thermal efficiency to 62.98%. Also, the use of red marble pieces reduced the distillate cost by 35.6% compared to the reference distiller.
Ieal Atresia and Total Colonic Hirschsprung Disease in a 36-week Neonate : Case Report
Authors: Atqiaee Khashayar , Hiradfar Mehran , Mashhadi Mahdi Parvizi , Khanghah Ali Samady 
Published: September 2022

Abstract
Intestinal atresia and Hirschsprung disease are two common causes of bowel obstruction in neonates, and simultaneous occurrence is rare.
This report delineates a 36-week newborn with ileal atresia and total colonic Hirschsprung.
A newborn male (gestational week 36; birth weight,2500 gram) was referred to our unit due to failure of meconium Passage during the first 48 hours after birth, abdominal distension, and bilious vomiting.
Physical examination revealed a prominent abdomen, decreased bowel sounds, and an empty rectum in digital rectal examination.
The abdominopelvic radiograph showed dilated intestinal loops. No gas was observed in the rectum.
Intraoperative findings were: Type three Ileal atresia with classic V shape deformity in the mesentery just 10 mm proximal to the ileocecal valve. All parts of the large bowel were unused (microcolon).
H&E examination of colonic biopsies revealed the absence of Ganglion cells in all parts of the large bowel.
Analysis of hyperprogressive disease due to atezolizumab plus bevacizumab treatment in patients with advanced hepatocellular carcinoma
Authors: Yumita Sae , Ogasawara Sadahisa , Nakagawa Miyuki , Maruta Susumu , Okubo Tomomi , Itokawa Norio , Iino Yotaro , Obu Masamichi , Haga Yuki , Seki Atsuyoshi , Kogure Tadayoshi , Ishino Takamasa , Ogawa Keita , Fujiwara Kisako , Iwanaga Terunao , Fujita Naoto , Sakuma Takafumi , Kojima Ryuta , Kanzaki Hiroaki , Koroki Keisuke , Inoue Masanori , Kobayashi Kazufumi , Kiyono Soichiro , Nakamura Masato , Kanogawa Naoya , Saito Tomoko , Kondo Takayuki , Nakagawa Ryo , Nakamoto Shingo , Muroyama Ryosuke , Chiba Tetsuhiro , Itobayashi Ei , Atsukawa Masanori , Koma Yoshihiro , Azemoto Ryosaku , Ito Kenji , Mizumoto Hideaki , Kato Jun , Kato Naoya 
Published: September 2022

Abstract
Background
Hyperprogressive disease (HPD) is a phenomenon with greatly accelerated tumor growth and clinical deterioration rates compared to pre-therapy, in patients treated with immune checkpoint inhibitors (ICI). The aim of this study is to clarify the reality of HPD in patients with advanced hepatocellular carcinoma (HCC) who were treated with atezolizumab plus bevacizumab (Atez/Bev) using tumor dynamics.
Methods
Medical records of consecutive patients with advanced HCC who were treated with Atez/Bev were retrospectively reviewed. HPD was defined as a more than two- or fourfold increase in tumor growth rate (TGR) or tumor growth kinetics rate (TGKR) pre- and post-treatment. Overall survival (OS) and baseline characteristics with or without HPD were analyzed.
Results
A total of 85 patients were included in the analysis. When HPD was defined as a twofold of TGR or TGKR, 8 patients (9.4%, 8/85) had HPD and 11 had PD without HPD. A total of 5 patients (5.9%, 5/85) were diagnosed with HPD and 14 with PD without HPD when HPD was defined as a fourfold of TGR or TGKR. No significant difference was observed in the baseline characteristics and OS between HPD and non-HPD.
Conclusion
The prevalence of HPD in patients with advanced HCC treated with Atez/Bev was lower than those treated with nivolumab monotherapy. The HPD mechanism in ICI combined with antibodies targeting vascular endothelial growth factor (VEGF) remains to be elucidated.
Medication adherence and its impact on glycemic control in type 2 diabetes mellitus patients with comorbidity: A multicenter cross-sectional study in Northwest Ethiopia
Authors: Sendekie Ashenafi Kibret , Netere Adeladlew Kassie , Kasahun Asmamaw Emagn , Belachew Eyayaw Ashete 
Published: September 2022

Abstract
Background: Medication nonadherence in patients with chronic diseases, particularly in type 2 diabetes mellitus (T2DM) with comorbidity has continued to be the cause of treatment failure. The current study assessed medication adherence and its impact on glycemic control in T2DM patients with comorbidity.
Methods: An institutional-based multicenter cross-sectional study was conducted among T2DM patients with comorbidity at the selected hospitals in Northwest Ethiopia. Medication adherence was measured using a structured questionnaire of the General Medication Adherence Scale (GMAS). A logistic regression model was used to identify predictors of the level of medication adherence and glycemic control. P < 0.05 at 95% confidence interval (CI) was statistically significant.
Results: A total of 403 samples were included in the final study. This study showed that more than three-fourths (76.9%) of the participants were under a low level of medication adherence. The source of medication cost coverage [AOR=10.593, 95% CI (2.628–41.835; P = 0.003], monthly income (P < 0.00), self-monitoring of blood glucose (SMBG) practice [AOR=0.266, 95% CI (0.117–0.604); P = 0.002], the number of medications [AOR=0.068, 95% CI (0.004–0.813); P = 0.014] and number of medical conditions [AOR = 0.307, 95% CI (0.026–0.437); P = 0.018] were found to be significant predictors of medication adherence. Significantly, most (74.7%) of the study participants had poor levels of glycemic control. Patients who had a high level of medication adherence [AOR = 0.003, 95% CI (0.000–0.113); P = 0.002] were found less likely to have poor glycemic control compared to patients who were low adherent to their medications.
Conclusion: The current study concluded that medication adherence was low and significantly associated with poor glycemic control. Therefore, management intervention and management of T2DM patients with comorbidity should focus on the improvement of medication adherence and other predictor variables.
Swallowing Disorders and Mortality in Adults with Advanced Cancer Outside the Head and Neck and Upper Gastrointestinal Tract: A Systematic Review
Authors: Silva Danielle Nunes Moura , Vicente Laélia Cristina Caseiro , Glória Vanessa Laís Pontes , Friche Amélia Augusta de Lima 
Published: September 2022

Abstract
Background

Although oncological palliative care is increasingly being offered by multidisciplinary teams, there is still a lack of data about some symptoms handled by these teams, such as dysphagia, in patients with advanced cancer outside swallow regions. This study aimed to estimate the occurrence of dysphagia in prognosis studies of adults with advanced cancer outside the head, neck, and upper gastrointestinal tract, and to determine if there is an association with mortality.

Methods

A systematic review of studies that evaluated dysphagia and mortality was conducted (PROSPERO: CRD42021257172). Data sources: BVS, PubMed, CINAHL, Web of Science, and Scopus. Data between January 1, 2011 and June 30, 2021 were selected for the current study.

Results

Among the 408 articles screened, only 11 were included. Dysphagia demonstrated a variable frequency, and almost half of the studies found a percentage of dysphagia above 60%, appearing most as a symptom that affects health-related quality of life and prove to be a toxicity of treatment. The association between dysphagia and mortality was only evaluated in two articles that studied advanced lung cancer, in which, after controlling for covariates, swallowing disorders were associated with worse survival, with hazard ratios of 1.12 and 1.34.

Conclusions

The occurrence of dysphagia in advanced cancer outside anatomical swallowing regions is common, and there seems to be an association with significantly decreased survival in patients with advanced lung cancer.
Comprehensive Analysis of Differentially Expressed Circular RNAs in Keloid Dermal Tissues
Authors: Liu Yunhan , Zhang Zewei , Huang Xin , Gu Shuchen , Khoong Yimin , Li Zhouxiao , Xia Wenzheng , Zan Tao 
Published: September 2022

AbstractBackground Keloid is a dermal fibroproliferative disease with various etiologies and unclear pathogenesis. Recent studies have revealed that circular RNAs (circRNAs) exerted regulatory functions through a competing endogenous RNA (ceRNA) pathway in keloid progression. However, the expression profiles of circRNAs in keloid dermal tissues (KDTs) remain unknown. This study aimed to identify differentially expressed circRNAs (DECs) and genes (DEGs) in KDTs, as well as to investigate the potential biological functionsof circRNAs based on the circRNA-miRNA-mRNA ceRNA network.ResultsThrough high-throughput RNA sequencing (RNA-seq), we revealed 3467 DEGs (865 up- and 2602 down-regulated) and 330 DECs (162 up- and 168 down-regulated) in KDTs. To reveal the functions of DECs preliminarily, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed for the host genes. Further, the up- and down-regulated DECs-miRNAs-DEGs regulatory networks were constructed, respectively. The functional prediction for the target genes showed that the up-regulated ceRNA network was associated with extracellular matrix and multiple cellular functions. The down-regulated ceRNA network was enriched in cell-cell junction and other biological processes. Cytoscape was used to visualize each network's protein-protein interaction (PPI) network and identify hub genes. By quantitative Real-Time PCR (qRT-PCR), hsa_circ_0060927, hsa_circ_0071410, hsa_circ_0058092, hsa_circ_0002874, hsa_circ_0004682, hsa_circ_0072688, hsa_circ_0006401, and hsa_circ_0055954 were identified significantly up-regulated in KDTs. Within, hsa_circ_0072688, which was up-regulated both in KDTs and keloid dermal fibroblasts (KDFs), and located in the cytoplasm, might be a key circRNA and affect the progression of keloid by impacting extracellular matrix, cell adhesion, and cell apoptosis, etc.ConclusionThis study not only filled a gap in the circRNA library of KDTs but also laid a foundation for probing the biological function of DECs in keloids. Hsa_circ_0072688 was thought to be a key circRNA and more experimental support is needed.
Effect of different dietary protein levels on goats’ blood parameters of Tswana goats reared in extensive production systems
Authors: Tsheole Mpho Sylvia , Mwanza Mulunda 
Published: September 2022

AbstractIn this study, an experiment was conducted to determine the impact of different dietary protein levels on a goat’s blood parameters. Twenty-four female Tswana weaner goats with similar body weights and ages (3 months old and 10.56±1.28 kg) were used for the experiment. Animals were grouped into three treatment groups of eight goats each in a randomised block design according to live weight. Animals were fed protein 23.51g/kg and energy 8.55g/kg DM and then given Lucernead libitum. Blood samples were collected on the first day of the experiment and then weekly until the end of the experiment (365) days. Samples collected were analysed for blood biochemistry a hematologicalcal parameters. Different levels of protein supplementation in goats significantly affected blood glucose, albumin, albuglobulin and urea (P<0.05). It was also noted that hematological parameters were influenced significantly (P<0.05) by the physiological stages of animals. Supplementation of protein influenced blood parameters, which gave an indication of the nutrition and energy status of animals. Incidences of fluctuations in some parameters were observed during this experiment and could have resulted from undetected minor infections, and weather extremities in the study area. The findings revealed that there was no impact on different diet levels of protein supplementation on the health status of animals hence, farmers could be advised to supplement with nutrient density which is cheaper. However, the effects of protein supplementation could also explain the effects on reproduction parameters.
Comparative Transcriptome Sequencing Analysis to Postulate the Scheme of Regulated Leaf Coloration in Perilla frutescens
Authors: Liu Xiaoning , Zhai Yanning , Liu Jingyu , Xue Jingqi , Markovic Tatjana , Wang Shunli , Zhang Xiuxin 
Published: September 2022

Abstract
Perilla as herb, ornamental, oil and edible plant is widely used in East Asia. Until now, the mechanism of regulated leaf coloration is still unclear. In this study, four different kinds of leaf colors were used to measure pigment contents and do transcriptome sequence to decipher the mechanism of leaf coloration. The measurements of chlorophyll, carotenoid, flavonoid, and anthocyanin showed that higher contents of all the above pigments were in full purple leaf ‘M357’, and they may be determined front and back leaf color formation with purple. Meanwhile, the content of anthocyanin was controlled back leaf coloration. The chromatic aberration analysis and correlative analysis between different pigments and L*a*b* values analysis also suggested front and back leaf color change was correlated with the above four pigments. The genes involved in leaf coloration were identified through transcriptome sequence. The expression levels of chlorophyll synthesis and degradation related genes, carotenoid synthesis related genes and anthocyanin synthesis genes showed up-/down-regulated expression in different color leaves and were consistent of these pigments’ accumulation. It was suggested that they were the candidate genes regulated perilla leaf color formation, and genes including F3’H, F3H, F3’,5’H, DFR, and ANS are probably important for regulating both front and back leaf purple formation. Transcription factors involved in anthocyanin accumulation, and regulating leaf coloration were also identified. Finally, the probable scheme of regulated both full green and full purple leaf coloration and back leaf coloration was postulated.
Strategic Leadership Change and Corporate Social Performance and Financial Distress as Moderating Role: Evidence from Emerging Economies
Authors: Hussain Shahid , Rasheed Abdul 
Published: September 2022

Abstract
Exchange in strategic leadership has necessary insinuations in place of corporate social performance and acceptability. Such as, new chief executive officers ensure a robust inducement to join massive investors to shape their acceptance as accurate with and reputation inside the firm. We look at the Salience Theory of Stakeholder to look at a state line circumstance, the financial distress presence, which could create a new chief executive officer's potential to perform this task. We amalgamated the records with the South Asia record to achieve basic statistics up to the firm-level. This stage furnished 9,647 observations for 759 organizations. We discuss the differential impacts among externally recruited chief executive officers (outsiders) and internally promoted chief executive officers (insiders) beneath the financial distress’ situation. We discuss that after organizations revel in financial distress; chief executive officers (outsiders) may rapidly transfer their interest and place in order hobbies of the shareholders on investors than chief executive officers (insiders). Our observation supports the strategic leadership and corporate social performance literature via offering the latest visions mad about how the turnover of corporate management and the context of firms could mutually form innovative decision-making of chief executive officers in the engagement of corporate social performance.
Tobacco cigarettes smoking and e-cigarettes using among university students: a cross-section survey in Guangzhou, China, 2021
Authors: Song Hongjia , Yang Xuemin , Yang Wanchun , Duan Kun , Jiang Xingtao , Huang Guangye , Li Min , Liu Peiqing , Chen Jianwen 
Published: September 2022

Abstract
Background
There is an increase in the use of nicotine products worldwide, and similar trends may be observed in young adults. Cigarettes are consumed most heavily in China. However, there is limited information about Chinese nicotine product users and trends in nicotine product use among university students. Therefore, our objectives were to investigate the using status of nicotine products and smoking behavior among the students from 7 universities in Guangzhou, China.
Methods
Students at 7 different universities in Guangzhou were surveyed online in 2021 through a cross-sectional survey. A total of 10008 students were recruited and after screening, 9361 participants were adopted in our statistics. Descriptive analysis, Chi-square analysis, and multivariate logistic regression analysis were used to explore the smoking status and influencing factors.
Results
A total of 58.3% of participants were male. 29.8% of the participants used nicotine products. The average age of the 9361 university students was 22.4 years (SD = 3.6). Among the users of nicotine products, 16.7% were e-cigarettes only users, 35.0%were tobacco cigarettes only users, and 48.3% were dual users.
Males were more likely to use nicotine products. Medical students, students from prestigious Chinese universities, and students with higher levels of education were less likely to use nicotine products. Similarly, students with unhealthy lifestyles (e.g., drinking alcohol, playing games excessively, staying up late) were more likely to use nicotine products. Emotion can have significant impacts on both tobacco cigarettes and e-cigarettes dual users when choosing nicotine products to use. More than half of dual users said they would choose tobacco cigarettes when they were depressed and e-cigarettes when they were happy.
Conclusion
We identified factors influencing the use of nicotine products among university students in Guangzhou, China. Gender, education level background, specialization, lifestyle habits and emotion all influenced the use of nicotine products among university students in Guangzhou, China. Male, low education level, from non-prestigious Chinese universities or vocational schools, non-medical specialization, and presence of unhealthy lifestyles were susceptibility factors for the use of tobacco cigarettes and e-cigarettes among university students in Guangzhou. Besides, emotions can influence dual users' choice of products.
The dual role of TRIM3 in colorectal cancer dependent on p53 status by retaining p53 in the cytoplasm to decrease its expression in the nuclei
Authors: Han Yang , Lu Su , Song Chenlong , Xuan Yi , Zhang Meng , Cai Hong 
Published: September 2022

Abstract
Colorectal cancer is a very heterogeneous disease caused by the interaction of genetic and environmental factors. P53, a few exceptions, is a frequent mutation and plays a critical role in the adenoma-carcinoma transition during the tumorous pathological process. Our team screened the series of genes’ expression by high-content screening techniques to discover TRIM3 a newly tumor-associated gene in CRC. TRIM3 demonstrated both tumor-suppressive and tumorigenic features dependent on p53 wild or mutant status in cell experiments in our study. TRIM3 could directly interact with the C terminus of p53 (residues 320 to 393), a common segment of wtp53 and mutp53. Moreover, TRIM3 could decrease p53 levels in the nuclei by retaining them in the cytoplasm to exert different neoplastic biological features in a wtp53 or mutp53 dependent pathway. Chemotherapy resistance develops in nearly all patients with advanced CRC and seriously limits the therapeutic efficacies of anticancer drugs. TRIM3 could successfully reverse the chemotherapy resistance of oxaliplatin in mutp53 CRC cells by decreasing mutp53 in the nuclei to downregulate the multidrug resistance gene. Therefore, TRIM3 could be a potential therapeutic strategy to improve the survival of CRC patients with mutp53 by degradation mutp53 in the nuclei.