bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stem cells facilitate gastric cancer cell growth and oxaliplatin resista nce via upregulation of SMARCA5
Authors: Nie Yongli , Guo Jun , Chen Jun , Wang Yanyan 
Published: May 2022

Purpose: To evaluate the role of mesenchymal stem cells (MSCs) in gastric cancer (GC), and reveal its underlying mechanisms.
Methods: Expression of SMARCA5 was quantified in GES-1, AGS and MKN45 cell lines. In MSCs-GC co-cultured cells, cell viability was assessed using cell counting kit-8 (CCK-8) assay, and colony formation were performed to determine cell proliferation. The invasion of the MSCs-GC cells was evaluated by Transwell assay. The levels of vimentin, snail and slug, as well as Wingless-Int (Wnt)/β-catenin related proteins β-catenin, Axin, c-myc, and matrix metalloproteinase (MMP)-7 were determined using immunoblotting. Oxaliplatin was added to GC cells, and the effect of siSMARCA5 on cell sensitivity to oxaliplatin was investigated after transfection with SMARCA5 siRNA into MSCs-GC cells.
Results: SMARCA5 was highly expressed after co-culture with MSCs (p < 0.001). The MSCs facilitated the proliferation of GC cells, and enhanced cell invasion as well as migration (p < 0.001) compared with untreated cells. MSCs also promoted epithelial–mesenchymal transition (EMT) by increasing production of vimentin, snail, and slug (p < 0.001). The MSCs decreased the sensitivity of GC cells to oxaliplatin, while the knockdown of SMARCA5 reversed the effect (p < 0.001). Furthermore, MSCs up-regulated β-catenin, c-myc, and MMP-7 levels, but downregulated Axin expression compared with untreated cells (p < 0.001). However, siSMARCA5 blocked these processes compared with si-NC group (p < 0.001).
Conclusion: MSCs facilitate GC cell growth and oxaliplatin resistance by upregulating SMARCA5 and activating Wnt/β-catenin signaling. This may provide an alternative treatment target for patients with GC.
NR4A1 alleviates the toxicity in neural stem cells induced by propofol in early life by regulating AMPK pathway
Authors: Zhu Miaomiao , Hu Jing , Gao Baofeng , Liu Changlin , Li Huiqing , Zhang Zengzhen 
Published: May 2022

Purpose: To examine the association of propofol (PRO) with related key genes that may serve as potential biomarkers for alleviation of PRO-induced toxicity in neural stem cells (NSCs).
Methods: Differentially expressed genes (DEGs) were screened based on GEO database, using the analysis platform of Metaboanalyst, GEO2R, DAVID and Ehbio. NSCs were purchased and treated with 3 μM of propofol (PRO). NR4A1 was transfected into NSCs, and the NR4A1 expression, apoptosis-related protein and AMPK pathway-related protein were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting. Cell viability and apoptosis were evaluated by methylthiazolyldiphenyl-tetrazolium bromid (MTT) assay and flow cytometry.
Results: A total of 278 DEGs were analyzed on GSE106799 microarray, and finally screened for differentially expressed down-regulated gene, NR4A1, which is a hubgene. NR4A1 expression decreased in PRO-induced NSCs. Furthermore, NR4A1 attenuated the PRO-induced decrease in the viability of NSCs and the increase in apoptosis. Moreover, NR4A1 increased p-AMPK/AMPK level.
Conclusion: NR4A1 attenuates the toxicity in NSCs induced by PRO by regulating AMPK pathways, and thus provides a theoretical basis for the treatment of nerve damage caused by anesthetics.
Empirical observations of the yield of logs from trees of the boreal region
Authors: Kärenlampi Petri P. 
Published: May 2022

The yield of sawlogs and plywood logs from boreal trees is discussed. First, taper curves are used to compute geometric (maximal) sawlog and plywood log contents. Then, a quality reduction is implemented, according to previously published models. Finally, the outcome is verified using empirical observations. Regarding spruce trees, the different estimates of sawlog content agree. In the case of birch and pine trees, the estimates differ. The sawlog and plywood log yield from large trees is greater than prognosticated one, probably due to missing over-aged trees in the empirical data. The yield of sawlogs and plywood logs from small trees is smaller than prognosticated one, most of the empirical data originating from thinnings with a focus on the quality of the remaining trees. The sawlog content of individual trees from clearcutting displays a skew distribution around the expected value. The distribution in thinning trunks shows a binary component, with a large proportion of trees with zero sawlog content. Keywords: sawlog; plywoodlog; Picea abies; Betula species; Pinus sylvestris
Social Practice and the Exchange of Metals and Metallurgical Knowledge in 2nd Millennium Central Asia
Authors: Stöllner Thomas , Gontscharov Anton 
Published: May 2022

The current article discusses the Bronze Age metal evidence in Central Asia based on a vast study of metals of Kazakh origin in order to better understand what Chernykh once called the West-Asian-Metallurgical Province (WAMP). Based on typological studies it became obvious that typologies do not sufficiently help to understand the distribution patterns of Bronze Age metals in regard to their social nor their economic background. The authors therefore propose an anthropological and theoretical approach that allows the exploration of the practice of exchange within steppe communities based on provenance studies of metals using elemental and Pb-isotope data. These data have been analysed within a research project carried out with Kazakhstan partners between 2004 and 2014. For the first time, a selection of data are presented that support some of the general interpretations of exchange modes between the Petrovka Early Bronze Age and the Late Bronze Age. Especially during the second millennium, it seems that the exchange pattern of metals had changed from single high valued items to a larger scale trade, which included metal transport as well. It is suggested that although the practice of exchange modes between the steppe communities change to larger scale metal exchange during the 2nd half of the 2nd millennium BC, most of the social background still remained similar in comparison to the earlier periods.
Educación Socioemocional y Formación Docente
Authors: García Pérez Jorge Luis , Garza Ruíz Bertha Alicia 
Published: May 2022

Se presenta un estudio con enfoque cualitativo y bajo una metodología de investigación-acción cuyo objetivo fue diseñar e implementar una estrategia de innovación que favorezca a la formación docente en el área de Educación Socioemocional a través de las redes sociales. El alcance del estudio es exploratorio y correlacional. Se creó una página de Facebook en donde se crearon y compartieron videos de carácter informativo sobre la materia en cuestión. Los videos fueron reproducidos en 15 estados de México con un total de 11,091 reproducciones. Para medir el impacto de la estrategia se implementó un grupo focal con cuatro docentes de un centro educativo de educación primaria, mismo que fue analizado a través de la herramienta Atlas.ti. Se concluye que a través de las redes sociales se pueden implementar estrategias de formación docente y que estas pueden lograr alcance e impacto entre la comunidad educativa.
Abstract
Research is presented containing a qualitative approach under an action research methodology aimed at the design and implementation of an innovation strategy favoring teacher training in the area of socioemotional education by means of social networks. The scope of this study is exploratory and correlational. A Facebook account was created where informative videos on the subject in question were created and shared. The videos were reproduced in 15 states in Mexico with a total of 11 091 reproductions. To measure the impact of the strategy, a focus group was implemented which consisted of four teachers from an elementary school in Nuevo Leon, Mexico. The results were analyzed using ATLAS.ti tool. It was concluded that by means of social networks teacher training strategies can be implemented and may achieve coverage and impact the educational community.
Dondurulmuş Deniz Ürünlerinde Vibrio spp.’nin İlmiğe Dayalı İzotermal Amplifikasyon (Loop Mediated Isothermal Amplification, LAMP) Yöntemiyle Belirlenmesi
Authors: DOĞRUER Yusuf , TELLİ A. Ezgi , TELLİ Nihat , BİÇER Yusuf 
Published: May 2022

Bu çalışmada, deniz ürünleri kaynaklı enfeksiyonlarda en yaygın etkenlerden olan Vibrio spp.’nin yüksek risk grubunu oluşturan Vibrio parahaemolyticus, V. vulnificus ve V. cholerae’nın yüksek özgüllük, verimlilik ve hızlılık içeren yeni bir yöntem olan İlmiğe Dayalı İzotermal Amplifikasyon (Loop Mediated Isothermal Amplification, LAMP) yöntemiyle tespit edilmesi amaçlandı.
Araştırma materyali olarak, süpermarketler ve balık hallerinde satışa sunulan dondurulmuş deniz ürünleri (hamsi filetosu, bütün hamsi, dondurulmuş balık köfte, ahtapot, karides, kalamar, akivades midyesi, midye eti, kabuklu midye, uskumru filetosu, sardalya, surimi çubuk, vongole midyesi, çıtır kalamar halkaları, kaya levreği n = 212) toplandı. Klasik kültür yöntemiyle örneklerin izolasyonu için ISO / TS 21872-1: 2007 ve ISO 21872-2: 2007 prosedürleri kullanıldı. Şüpheli izolatlardan DNA ekstraksiyonu gerçekleştirildi. Daha sonra izolatlar Vibrio spp. için gyrB1 gen bölgesinin amplifikasyonu ile genus spesifik PCR reaksiyonu kullanılarak doğrulandı. Pozitif örneklerde turbidite bazlı Real-Time LAMP gerçekleştirildi. Bu amaçla, sırasıyla V. parahaemolyticus, V. vulnificus ve V. cholerae'nin saptanması için toxR, vvHA ve ompW hedef gen bölgeleri için tasarlanmış LAMP primer setleri kullanıldı.
Sonuç olarak, klasik kültür metodu kullanılarak analiz edilmiş 212 (% 16,98) örnekten 36'sının Vibrio spp. ile kontamine olduğu tespit edildi. LAMP reaksiyonu sonuçlarına göre, Vibrio spp. pozitif izolatların içerisinde V. parahaemolyticus ve V. vulnificus'un oranı sırasıyla 13/36 (% 36.1) ve 2/36 (% 5.5) olarak tespit edildi. Analiz edilen örneklerin hiçbirinde V. cholerae tespit edilmedi. Bu bağlamda, dondurulmuş deniz ürünlerinde V. parahaemolyticus ve V. vulnificus gibi patojenik suşların varlığı halk sağlığı açısından riskli olarak kabul edildi.
STUDENT LEARNING MOTIVATION IN ONLINE BASED PHYSICS LEARNING AT SMAN 1 AMANDRAYA
Authors: Buulolo Febrius , Pardede Hebron , Silaban Bajongga 
Published: May 2022

Penelitian ini bertujuan untuk mendeskripsikan motivasi belajar peserta didik pada pembelajaran fisika berbasis online sekolah menengah atas negeri 1 Amandraya. Metode penelitian yang digunakan adalah kuantitatif deskriptif dengan populasi seluruh peserta didik dan guru. Sampel penelitian ini terdiri 85 peserta didik dan 2 guru yang dipilih menggunakan teknik purposive sampling. Pengumpulan data penelitian menggunakan angket melalui Google Form dan Whatsapp diberikan kepada 43 item untuk peserta didik dan 15 item untuk guru. Hasil penelitian motivasi belajar fisika peserta didik diperoleh dan dianalisis berdasarkan respon peserta didik dan guru, yaitu pada indikator pada cita-cita dan aspirasi peserta didik tergolong baik, kemampuan peserta didik tergolong cukup baik, kondisi peserta didik tergolong baik, upaya guru dalam membelajarkan peserta didik tergolong sangat baik, kondisi lingkungan peserta didik tergolong baik, fasilitas belajar peserta didik tergolong cukup baik. Keseluruhan indikator motivasi belajar fisika berdasarkan respon peserta didik dan guru pada pembelajaran fisika berbasis online tergolong baik.
Pandemic-activated Psychological Growth: Significance of extraversion, self-consciousness and COVID-19 related anxiety
Authors: Łoś Zbigniew , Żurko Magdalena , Słowińska Aleksandra , Senejko Alicja , Madeja-Bień Kamila 
Published: May 2022

BackgroundThe sense of threat to health and life in the face of pandemic, accompanied by difficulties imposed by lockdown, may trigger a serious crisis. Among possible consequences of such a crisis may paradoxically be the phenomenon of psychological growth. The aim of this article is to identify predictors of pandemic-activated psychological growth (PPG). The relationships between extraversion, reflective and ruminative self-consciousness and the PPG were the subject of our inquiry. Additionally, a question was posed about the indirect effect of self-consciousness on the PPG through anxiety.Participants and procedureParticipants: The study involved 1206 participants aged 18 to 26 years, who declared that the pandemic situation significantly threatens their important life goals.
Procedure: Cross-sectional design. Four online short questionnaire-measures were used: The Ten-Item Personality Inventory (TIPI), The Rumination-Reflection Questionnaire (RRQ), The Current Self-disposition Scale (CSS), and The Post-traumatic Growth Inventory (PTGI).ResultsExtraversion and reflective self-consciousness were direct predictors of higher PPG. Whereas ruminative self-consciousness was directly related to a lower PPG. There was the indirect effect of ruminative self-consciousness on the PPG through the COVID-19 related anxiety.ConclusionsAlthough the results do not allow to conclude on the permanence of growth effect, finding the PPG predictors considered as beneficial resources for coping with difficult pandemic circumstances appears to be valuable in the current state of affairs.
Immune and inflammatory cytokines profile in Iraqi patients with acute and chronic myeloid leukemia
Authors: M. J. AL-Khateeb Shatha , Thair Tahir Noor , Al-bayati Ali Abdulateef Hasan 
Published: May 2022

Introduction and Aim: Evaluation of the profile of immunological and inflammatory cytokines, IL-6, IL-2 and TNF? in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) patients compared their level with healthy controls, and assessment of the association of these cytokines with clinical and hematological variables.

Patients and Methods: Thirty AML, thirty CML patients, and thirty healthy control individuals were recruited for this study from the National Hematology Center for Research and Treatment in Baghdad for the period of October 2020 to March 2021. Leukemic patients were under chemotherapy. Complete blood picture routine test and cytokines profile test were done. IL-6, IL-2 and TNF-? were quantitatively measured using enzymes linked immunosorbent assay (ELISA) kits.

Results: Hematological myeloid cancers affect more males than female, in AML male/female ratio was 17/13 while in CML was 16/14. The range of duration of the disease for both AML and CML was 1-3 years. in both AML group the level of IL-6 59.66 ± 31.99 pg/mL and in CML group 57.07± 16.52 pg/mL were both significantly higher P value < 0.001 than that of control group 37.95± 6.10 pg/mL. TNF? in AML group was significantly higher P value < 0.001 than that of control group. A high significant difference in serum level of IL-2 P value <0.0001 in AML and CML compared to controls.

Conclusion: IL-6, TNF? and IL-2 levels were higher in myeloid malignancy groups than controls. Serum cytokines shown to be positively associated with each other and could signify to poor outcome of the myeloid malignancy patients.
Efficacy of herbal medicine for patients with diabetic neuropathies: An updated literature review
Authors: Hutapea Albert Manggading , Simbolon Boyke Marthin 
Published: May 2022

Traditional medicine is efficacious to protect complications of diabetes mellitus, such as diabetic neuropathy. Therapy with herbal medicine has been developed for people with diabetes empirically over the years. This literature review aims to assess effect of herbal medicine for patients with diabetic neuropathies. We conducted a literature analysis using articles that were randomized controlled clinical trials (RCTs). Three databases were searched from PubMed, EMBASE, CENTRAL that have been published from January 2000 until October 2021until October 2021 and obtained four articles that met the study criteria. The review analysis results showed a significant effect between Gua Sha therapy, green tea extract, and topical Citrullus colocynthis on reducing diabetic neuropathy pain (p < 0.05). Nutmeg Extracts did not show significant results on diabetes mellitus pain after being treated for four weeks (p = 0.063). The review results in the physical and neural function also showed the significant increase in mean scores. Despite the positive findings, it is too early to conclude on the efficacy of herbal medicine to treat diabetic neuropathy due to the high clinical heterogeneity and the number of sample size of the study was too small. However, it may be indicative of the effect of herbal medicine in reducing pain in diabetic neuropathy.
Effect of Ayurveda in management of dysbiosis with special reference to Bifidobacterium in children with autism spectrum disorders
Authors: K. S. Dinesh , C. Krishnendhu , Balakrishnan Geethu , Chitra S. Swapna , A. S. Santhi Krishna , M. J. George , Patel Anita 
Published: May 2022

Introduction and Aim: The prevalence of autism spectrum disorder has risen in the last two decades. Disruption of homeostasis of several species and genus of bacteria in the gut termed as dysbiosis is having a pivotal role in ASD pathophysiology. Invariable correlations between the behavioral severities of ASD and dysbiosis management have been affirmed through various previous studies. Bifidobacterium make a significant species appraised well in microbial studies linked with ASD. A randomized controlled trial was conducted to assess the efficacy of Ayurveda therapies in improving Bifidobacterial abundance in Autism children.

Methodology: The study took place at the VPSV Ayurveda College Kottakkal's outpatient and in-patient units where 60 children with Autism were randomized to two groups. The intervention prolonged 30 days in total, with three rounds of assessment: baseline (0th day), interim (30th day), and final (60thday). Both the test and control groups received conventional multidisciplinary therapies for 60 days and additionally, the test group was given Ayurveda poly-herbal formulations and food and lifestyle instructions for first 30 days. The result was made through GI severity index measurement and 16SrRNA sequencing.

Results: After one month of intervention with poly herbal compounds of Ayurveda along with lifestyle guidelines and conventional therapies a statistically significant improvement observed in the relative abundance of Bifidobacterium in intervention group compared to control group.

Conclusion: Poly herbal compounds of Vilwadi Gulika and Rajanyadi choornam along with the dietary lifestyle guidelines and multidisciplinary interventions has played a big role in improving Bifidobacteria in autistic children.
Antibacterial efficacy of Lawsonia inermis (Henna) against the predominant endodontic pathogen (Enterococcus faecalis)-An in vitro antimicrobial assay
Authors: Elizabeth J.A. Amilia , Hemalatha R. , Shankar P. , Jayakaran Trophimus Gnanabagyan , Rajkumar M. 
Published: May 2022

Introduction and Aim: The prospect of a successful root canal therapy relies on three main factors which includes proper instrumentation, disinfection and obturation of root canal. The goal of the present study is to analyse the anti-microbial efficacy of henna (Lawsonia inermis) against the major endodontic pathogen, Enterococcus faecalis.

Materials and Methods: In this study, the antibacterial efficacy of Lawsonia inermis is investigated against Enterococcs feacalis using the method of agar disc diffusion. Extracts of L. inermis were prepared by using methanol as an extraction solvent, whereas DMSO (Dimethyl sulfoxide) and water were used as dissolution solvents. The diluted henna sample was used as the test sample, while positive control used was chlorhexidine gluconate solution and the negative control used was saline. The bacteria were cultured, and the samples were placed to measure the zone of inhibition.

Results: Extracts of L. inermis displayed noteworthy antimicrobial activity against E. fecalis. The zone of inhibition of henna against E. fecalis was found to be 11mm whereas the positive control had 14mm.

Conclusion: In conclusion, the henna extracts had shown acceptable antimicrobial efficacy and thus this study provides the scientific reason for the use of L. inermis in dentistry.
Roles and Functions of a Non-Academic Medical School Facebook Page from the Student Perspective: A Study of Usage and Survey Data
Authors: Siah Qi Zhuang , Sykes Ella , Golaup Caitlin , Browne Julie 
Published: May 2022

Background: Facebook is a well-established social networking platform that is commonly used by medical schools as an educational resource, but there are few studies assessing the roles of a non-academic Facebook page in medical education. Cardiff University uses Facebook primarily as a student support and engagement platform through its ‘C21’ Facebook Page; this study aimed to explore the use of the page by students, as well as their perceptions on the value of the page and the appropriateness of social media use by the medical school.
Methods:Authors collected and analyzed C21 Facebook Page usage data to obtain descriptive information on reach, engagement and content. They also distributed an anonymized survey to evaluate and explore users’ interest in, experience of and engagement with the content.
Results: Of the 1021 posts on the page in 2019, the highest post-engagement rate occurred in the?Student or Staff News?category (13.5%) and the lowest in?Medical Research News?(3.5%). Survey feedback on the page was overwhelmingly positive (n=89; 84.8%), and respondents reported a high degree of trust (n=95; 90.5%) in the page. Students would like to see more ‘Curriculum Vitae (CV)-building’ Opportunities advertised on the page.
Conclusion:The C21 Facebook Page is an important resource in developing community within the medical school and facilitating student engagement with both the C21 course and wider medical opportunities. It is perceived as an appropriate channel of communication between the medical school and students.
Reseña de la obra de Hernández Muñoz, Natividad, Muñoz-Basols, Javier y Soler Montes, Carlos. La diversidad del español y su enseñanza. Londres/Nueva York: Routledge, 2022. ISBN: 978-03-676-5169-5. 378 páginas.
Authors: Aijón Oliva Miguel Ángel 
Published: May 2022

Reseña de la obra de Hernández Muñoz, Natividad, Muñoz-Basols, Javier y Soler Montes, Carlos. La diversidad del español y su enseñanza. Londres/Nueva York: Routledge, 2022. ISBN: 978-03-676-5169-5. 378 páginas.