bluuebunny/crossref_metadata_2025_split huggingface dataset:
Focal Serous Tubal Intra-Epithelial Carcinoma Lesions Are Associated With Global Changes in the Fallopian Tube Epithelia and Stroma
Authors: Wu Jingni , Raz Yael , Recouvreux Maria Sol , Diniz Márcio Augusto , Lester Jenny , Karlan Beth Y. , Walts Ann E. , Gertych Arkadiusz , Orsulic Sandra 
Published: March 2022

ObjectiveSerous tubal intra-epithelial carcinoma (STIC) lesions are thought to be precursors to high-grade serous ovarian cancer (HGSOC), but HGSOC is not always accompanied by STIC. Our study was designed to determine if there are global visual and subvisual microenvironmental differences between fallopian tubes with and without STIC lesions.MethodsComputational image analyses were used to identify potential morphometric and topologic differences in stromal and epithelial cells in samples from three age-matched groups of fallopian tubes. The Benign group comprised normal fallopian tubes from women with benign conditions while the STIC and NoSTIC groups consisted of fallopian tubes from women with HGSOC, with and without STIC lesions, respectively. For the morphometric feature extraction and analysis of the stromal architecture, the image tiles in the STIC group were further divided into the stroma away from the STIC (AwaySTIC) and the stroma near the STIC (NearSTIC). QuPath software was used to identify and quantitate secretory and ciliated epithelial cells. A secretory cell expansion (SCE) or a ciliated cell expansion (CCE) was defined as a monolayered contiguous run of &gt;10 secretory or ciliated cells uninterrupted by the other cell type.ResultsImage analyses of the tubal stroma revealed gradual architectural differences from the Benign to NoSTIC to AwaySTIC to NearSTIC groups. In the epithelial topology analysis, the relative number of SCE and the average number of cells within SCE were higher in the STIC group than in the Benign and NoSTIC groups. In addition, aging was associated with an increased relative number of SCE and a decreased relative number of CCE. ROC analysis determined that an average of 15 cells within SCE was the optimal cutoff value indicating the presence of a STIC lesion in the tubal epithelium.ConclusionsOur findings suggest that global stromal alterations and age-associated reorganization of tubal secretory and ciliated cells are associated with STIC lesions. Further studies will need to determine if these alterations precede STIC lesions and provide permissible conditions for the formation of STIC.
Potential Therapeutic Targets of Rehmannia Formulations on Diabetic Nephropathy: A Comparative Network Pharmacology Analysis
Authors: Chan Kam Wa , Yu Kam Yan , Yiu Wai Han , Xue Rui , Lok Sarah Wing-yan , Li Hongyu , Zou Yixin , Ma Jinyuan , Lai Kar Neng , Tang Sydney Chi-wai 
Published: March 2022

Background: Previous retrospective cohorts showed that Rehmannia-6 (R-6, Liu-wei-di-huang-wan) formulations were associated with significant kidney function preservation and mortality reduction among chronic kidney disease patients with diabetes. This study aimed to investigate the potential mechanism of action of common R-6 variations in a clinical protocol for diabetic nephropathy (DN) from a system pharmacology approach.Study Design and Methods: Disease-related genes were retrieved from GeneCards and OMIM by searching “Diabetic Nephropathy” and “Macroalbuminuria”. Variations of R-6 were identified from a published existing clinical practice guideline developed from expert consensus and pilot clinical service program. The chemical compound IDs of each herb were retrieved from TCM-Mesh and PubChem. Drug targets were subsequently revealed via PharmaMapper and UniProtKB. The disease gene interactions were assessed through STRING, and disease–drug protein–protein interaction network was integrated and visualized by Cytoscape. Clusters of disease–drug protein–protein interaction were constructed by Molecular Complex Detection (MCODE) extension. Functional annotation of clusters was analyzed by DAVID and KEGG pathway enrichment. Differences among variations of R-6 were compared. Binding was verified by molecular docking with AutoDock.Results: Three hundred fifty-eight genes related to DN were identified, forming 11 clusters which corresponded to complement and coagulation cascades and signaling pathways of adipocytokine, TNF, HIF-1, and AMPK. Five variations of R-6 were analyzed. Common putative targets of the R-6 variations on DN included ACE, APOE, CCL2, CRP, EDN1, FN1, HGF, ICAM1, IL10, IL1B, IL6, INS, LEP, MMP9, PTGS2, SERPINE1, and TNF, which are related to regulation of nitric oxide biosynthesis, lipid storage, cellular response to lipopolysaccharide, inflammatory response, NF-kappa B transcription factor activity, smooth muscle cell proliferation, blood pressure, cellular response to interleukin-1, angiogenesis, cell proliferation, peptidyl-tyrosine phosphorylation, and protein kinase B signaling. TNF was identified as the seed for the most significant cluster of all R-6 variations. Targets specific to each formulation were identified. The key chemical compounds of R-6 have good binding ability to the putative protein targets.Conclusion: The mechanism of action of R-6 on DN is mostly related to the TNF signaling pathway as a core mechanism, involving amelioration of angiogenesis, fibrosis, inflammation, disease susceptibility, and oxidative stress. The putative targets identified could be validated through clinical trials.
Psychological Impact in Healthcare Workers During Emergencies: The Italian Experience With COVID-19 First Wave
Authors: Pisanu Elisabetta , Di Benedetto Annalisa , Infurna Maria Rita , Rumiati Raffaella I. 
Published: March 2022

BackgroundThe COVID-19 outbreak imposed an overwhelming workload as well as emotional burdens on Healthcare workers (HCWs). In May 2020, an online survey was administered to HCWs in Italy to assess the pandemic's psychological impact and to investigate possible predictive factors that led to individual differences.MethodsThe psychological experience was measured based on the prevalence of self-reported feelings during the pandemic, including negative and positive emotional states. We analyzed the relationship between factors of gender, age, geographic region, professional role, and operational unit, and the four-point scale used to rate the frequency of each emotional state experienced by performing several multinomial logistic regressions, one for each emotion.ResultsOur findings suggest that more than half of HCWs experienced psychological distress during the first COVID-19 outbreak in Italy. Female and younger respondents, especially those operating in northern Italy experienced more frequently negative emotional states such as irritability, anxiety, loneliness, and insecurity. However, positive feelings, first of all solidarity, were also reported especially by female and older workers. The majority of the negative as well as positive emotional states were experienced almost equally by both doctors and nurses, and independently of the operational unit in which they operated.ConclusionsThis study can be very useful as a contribution to the current literature on the psychological effects of this pandemic on health workers. Moreover, our findings can provide useful information in planning more tailored psychological interventions to support this category of workers in the ongoing and future emergencies.
The Effects of Plasma Homocysteine Level on the Risk of Three Major Psychiatric Disorders: A Mendelian Randomization Study
Authors: Yu Jing , Xue Ranran , Wang Qiuling , Yu Hao , Liu Xia 
Published: March 2022

BackgroundHigher homocysteine (Hcy) level has been suggested to be associated with major psychiatric disorders (MPDs), such as schizophrenia (SCZ), bipolar disorder (BD), and major depressive disorder (MDD). We investigated the causal relationships between plasma Hcy level and MPDs risks using the Mendelian randomization (MR) method.MethodsWe selected 18 loci associated with plasma Hcy level from a large-scale genome-wide association study (GWAS) as genetic instruments. Genetic associations with SCZ, MDD, BD and BD subtypes (BD-I and BD-II) were extracted from several GWAS datasets from the Psychiatric Genomics Consortium. We used the Generalized Summary-data-based Mendelian Randomization (GSMR) method to estimate the associations of genetically predicted plasma Hcy levels with MPDs risks. We also performed inverse variance-weighted (IVW) analysis to verify the GSMR results and used MR-Egger regression and leave-one-out analysis to test the assumptions for a valid MR analysis.ResultsGenetically predicted plasma Hcy levels were associated with risks of SCZ (odds ratio [OR] = 1.12,PGSMR= 1.73 × 10−3) and BD-I (OR = 1.14,PIVW= 5.23 × 10−3) after Bonferroni correction. These associations were statistically significant when using IVW analysis (SCZ: OR = 1.11,PIVW= 2.74 × 10−3; BD-I: OR = 1.13,PIVW= 9.44 × 10−3). Furthermore, no significant horizontal pleiotropy was found by sensitivity analysis, and leave-one-out analyses showed no specific SNP affected the overall estimate. However, genetically determined plasma Hcy levels were not causally associated with MDD, BD, or BD-II risks.ConclusionOur results suggest that elevated plasma Hcy levels may increase the risk of SCZ or BD-I. Further randomized clinical trials are warranted to validate the MR findings in our study.
Establishment of a Dynamic Nomogram for Predicting the Risk of Lymph Node Metastasis in T1 Stage Colorectal Cancer
Authors: Liu Zitao , Huang Chao , Tian Huakai , Liu Yu , Huang Yongshan , Zhu Zhengming 
Published: March 2022

BackgroundAccurate prediction of the risk of lymph node metastasis in patients with stage T1 colorectal cancer is crucial for the formulation of treatment plans for additional surgery and lymph node dissection after endoscopic resection. The purpose of this study was to establish a predictive model for evaluating the risk of LNM in patients with stage T1 colorectal cancer.MethodsThe clinicopathological and imaging data of 179 patients with T1 stage colorectal cancer who underwent radical resection of colorectal cancer were collected. LASSO regression and a random forest algorithm were used to screen the important risk factors for LNM, and a multivariate logistic regression equation and dynamic nomogram were constructed. The C index, Calibration curve, and area under the ROC curve were used to evaluate the discriminant and prediction ability of the nomogram. The net reclassification index (NRI), comprehensive discriminant improvement index (IDI), and clinical decision curve (DCA) were compared with traditional ESMO criteria to evaluate the accuracy, net benefit, and clinical practicability of the model.ResultsThe probability of lymph node metastasis in patients with T1 colorectal cancer was 11.17% (20/179). Multivariate analysis showed that the independent risk factors for LNM in T1 colorectal cancer were submucosal invasion depth, histological grade, CEA, lymphovascular invasion, and imaging results. The dynamic nomogram model constructed with independent risk factors has good discrimination and prediction capabilities. The C index was 0.914, the corrected C index was 0.890, the area under the ROC curve was 0.914, and the accuracy, sensitivity, and specificity were 93.3, 80.0, and 91.8%, respectively. The NRI, IDI, and DCA show that this model is superior to the ESMO standard.ConclusionThis study establishes a dynamic nomogram that can effectively predict the risk of lymph node metastasis in patients with stage T1 colorectal cancer, which will provide certain help for the formulation of subsequent treatment plans for patients with stage T1 CRC after endoscopic resection.
A Perioperative Paradigm of Cranioplasty With Polyetheretherketone: Comprehensive Management for Preventing Postoperative Complications
Authors: He Zhenghui , Ma Yuxiao , Yang Chun , Hui Jiyuan , Mao Qing , Gao Guoyi , Jiang Jiyao , Feng Junfeng 
Published: March 2022

IntroductionAt present, lots of studies have discussed the effects and outcomes of cranioplasty using polyetheretherketone (PEEK). However, interventions or management for PEEK cranioplasty got less attention. This article presented a perioperative paradigm for preventing postoperative complications.Materials and MethodsModified PEEK plates with certified safety were implanted in patients who received evolving perioperative paradigm. Serial perioperative managements were developed as a comprehensive paradigm to prevent correlated risk factors of postoperative complications, which mainly included managements of epidural collections and wound healing. The preparation of the surgical area and systemic state were essential before surgery. During the operation, the blood supply of the incision and the handling of dura and temporalis were highlighted in our paradigm. After cranioplasty, management of subcutaneous drainage and wound healing were stressed. Patients received conventional management from February 2017 to August 2018 in our center. After the evolving paradigm developed, patients received comprehensive perioperative management from September 2018 to August 2020.ResultsA total of 104 patients who underwent PEEK cranioplasty were consecutively enrolled; 38 (36.5%) received conventional perioperative management, and 66 (63.5%) received evolving perioperative paradigm. The general information of the two groups was comparable. Notably, patients who received the evolving paradigm presented a significantly decreased incidence of postoperative complications from 47.4 to 18.2% (P &lt; 0.01), among which the incidences of subcutaneous effusion, epidural hematoma, and subcutaneous infection decreased significantly.ConclusionThe evolving perioperative paradigm could effectively prevent risk factors and reduce related complications. It was valuable to promote these comprehensive managements and inspire more clinical practice on improving patients' outcomes after PEEK cranioplasty.
Combined Use of Presepsin and (1,3)-β-D-glucan as Biomarkers for Diagnosing Candida Sepsis and Monitoring the Effectiveness of Treatment in Critically Ill Patients
Authors: Dobiáš Radim , Káňová Marcela , Petejová Naděžda , Pisti Štefan Kis , Bocek Robert , Krejčí Eva , Stružková Helena , Cachová Michaela , Tomášková Hana , Hamal Petr , Havlíček Vladimír , Raška Milan 
Published: March 2022

New biomarker panel was developed and validated on 165 critically ill adult patients to enable a more accurate invasive candidiasis (IC) diagnosis. Serum levels of the panfungal biomarker (1,3)-β-D-glucan (BDG) and the inflammatory biomarkers C-reactive protein, presepsin (PSEP), and procalcitonin (PCT) were correlated with culture-confirmed candidemia or bacteremia in 58 and 107 patients, respectively. The diagnostic utility was evaluated in sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). BDG was the best marker for IC, achieving 96.6% sensitivity, 97.2% specificity, 94.9% PPV, and 98.1% NPV at a cut-off of 200 pg/mL (p ≤ 0.001). PSEP exhibited 100% sensitivity and 100% NPV at a cut-off of 700 pg/mL but had a lower PPV (36.5%) and low specificity (5.6%). Combined use of PSEP and BDG, thus, seems to be the most powerful laboratory approach for diagnosing IC. Furthermore, PSEP was more accurate for 28-day mortality prediction the area under the receiver operating characteristic curve (AUC = 0.74) than PCT (AUC = 0.31; PCT cut-off = 0.5 ng/mL). Finally, serum PSEP levels decreased significantly after only 14 days of echinocandin therapy (p = 0.0012). The probability of IC is almost 100% in critically ill adults with serum BDG and PSEP concentrations > 200 pg/mL and >700 pg/mL, respectively, defining a borderline between non-invasive superficial Candida colonization and IC.
Repurposing Avasimibe to Inhibit Bacterial Glycosyltransferases
Authors: Hasan Md Kamrul , El Qaidi Samir , McDonald Peter , Roy Anuradha , Hardwidge Philip R. 
Published: March 2022

We are interested in identifying and characterizing small molecule inhibitors of bacterial virulence factors for their potential use as anti-virulence inhibitors. We identified from high-throughput screening assays a potential activity for avasimibe, a previously characterized acyl-coenzyme A: cholesterol acyltransferase inhibitor, in inhibiting the NleB and SseK arginine glycosyltransferases from Escherichia coli and Salmonella enterica, respectively. Avasimibe inhibited the activity of the Citrobacter rodentium NleB, E. coli NleB1, and S. enterica SseK1 enzymes, without affecting the activity of the human serine/threonine N-acetylglucosamine (O-GlcNAc) transferase. Avasimibe was not toxic to mammalian cells at up to 200 µM and was neither bacteriostatic nor bactericidal at concentrations of up to 125 µM. Doses of 10 µM avasimibe were sufficient to reduce S. enterica abundance in RAW264.7 macrophage-like cells, and intraperitoneal injection of avasimibe significantly reduced C. rodentium survival in mice, regardless of whether the avasimibe was administered pre- or post-infection. We propose that avasimibe or related derivates created using synthetic chemistry may have utility in preventing or treating bacterial infections by inhibiting arginine glycosyltransferases that are important to virulence.
Sistemas de reparación estructural de pavimento rígido con inyecciones de resinas epóxicas
Authors: Calizaya Romero Ariane Andreina 
Published: March 2022

El objetivo de este estudio fue proponer el uso de sistemas de reparación estructural para fisuras en pavimento rígido, a través de la aplicación de aditivo usando inyecciones de resina epóxica. Se trató de un estudio experimental prospectivo de corte longitudinal cuya metodología comprende seis pasos. Los materiales, condiciones físicas y la normativa responden al contexto de la ciudad de Sucre, Bolivia y a la necesidad de reparación de una avenida de esta ciudad. Como resultado se probó que la resina epóxica es altamente adherente, la resistencia a la reflexión es superior a 28 días que a 10 días de curado; la comparación de resultados obtenidos entre la primera y segunda ruptura demuestran poca variación. Las correcciones que usaron los sistemas de reparación permitieron demostrar que son una solución viable, óptima y eficaz para corregir estas patologías, constituyéndose en una alternativa para evitar la demolición innecesaria de las losas que presentan fallas.
DAMPAK PSIKOLOGIS PEMBELAJARAN DARING PADA MASA PANDEMI DI MIN 1 SUMENEP
Authors: Aisyah Siti 
Published: March 2022

The author examines the psychological impact of online learning during the pandemic. Psychology is the study of attitudes. The application of online learning in education, especially among students, has an impact on students' psychology. As a long-term result, students have a lack of social interaction with the surrounding environment, it reduced learning effectiveness, and students could easily feel bored. Online learning is a learning system that is carried out without meeting face to face. Online systems use media and technology that can connect one person to another remotely. The online learning system has been implemented since the pandemic. One of the schools that implements online learning is MIN 1 Sumenep. MIN 1 Sumenep School applies online learning using media such as Whatsapp, Zoom Meeting, and or other applications. In this study, the author uses a qualitative research method in which the instrument is the researcher herself. Therefore, the researcher, as an instrument, must be prepared her objectiveness how far the researchers are ready to carry out research before goes to the field. Data collection techniques were carried out by means of observation, interviews, and documentation. The data analysis uses descriptive analysis method or in other words describes a picture of the object under study through the data or samples that have been collected. The data analysis process begins by examining all available data from various sources.
Key words: Psychology of learning, online learning, education, pandemic, impact.
Pemahaman Magnetik pada Pelajaran Tematik di Sekolah Dasar
Authors: Rachman Natriya Faisal , Rozaq Fadli , Aghastya Adya , Astuti Septiana Widi , Wirawan Willy Artha , Adi Wahyu Tamtomo 
Published: March 2022

Belajar adalah satu kegiatan interaksi proses dari secara kondisi disekitar peserta didik. Seperti proses pengarahan untuk pencapaian tujuan dan proses melakukan perbuatan melalui pengalaman yang diciptakan diartikan pada belajar. Pengukuran / penilaian merupakan mencapai kompetensi. Penggunaan magnet dalam kehidupan sekarang ini tidak dapat dipungkiri. Sejak awal, penelitian dan pemahaman tentang magnet telah menghasilkan berbagai produk yang bermanfaat bagi umat manusia.. Motor listrik, generator listrik, satelit, lampu, perangkat pengangkat dan penarik benda logam pada pesawat angkat, hingga kereta api cepat adalah beberapa contoh penerapan magnet. Untuk memahami kemagnetan bagi siswa sekolah dasar dan penggunaan magnet, perlu dipahami siswa sekolah dasar. Hal ini bertujuan untuk pemahaman yang lebih baik tentang fungsi dan penggunaan magnet di sekitar kita dan pemahaman yang lebih baik sejak usia dini. Berdasarkan data survei, setelah kegiatan pengabdian masyarakat, pemahaman siswa banyak yang meningkat Berdasarkan data, bisa diketahui yang sebelum dilakukan pengabdian masyarakat adalah 70% siswa belum memahami tentang magnetik dan setelah melakukan kegiatan pengabdian masyarakat tentang pemahaman magnetik siswa SD di pelajaran tematik bisa diketahui 20% siswa belum memahami tentang magnetik, 46% memahami tentang magnetic dan 34% siswa sangat memahami tentang magnetik
Atmospheric and Fundamental Parameters of Eight Nearby Multiple Stars
Authors: Hussein Abdallah M. , Al-Wardat Mashhoor A. , Abushattal Ahmad , Widyan Hatem S. , Abu-Alrob Enas M. , Malkov Oleg , Barstow Martin A. 
Published: March 2022

Abstract
We present the complete set of atmospheric and fundamental parameters, in addition to the masses, for the individual components of eight stellar systems. The list of the systems, whose orbital solutions were published recently, includes seven binaries (HIP 14524, HIP 16025, HIP 46199, HIP 47791, HIP 60444, HIP 61100, HIP 73085) and one triple system (HIP 28671). The systems were analyzed using a spectrophotometric computational technique known as Al-Wardats method for analyzing binary and multiple stellar systems, which makes use of ATLAS9 line-blanketed plane-parallel model atmospheres. Using these estimated parameters, the positions of the components were located on the Hertzsprung–Russell diagram, evolutionary tracks and isochrones to estimate their ages, the range depending on the uncertainties in their metallicities. Five systems were found to be pre-main-sequence stars (HIP 14524, HIP 46199, HIP 60444, HIP 61100, HIP 73085), two were main-sequence stars (the zero-age HIP 28671 and the 6.3 Gyr HIP 16025), and one is a subgiant system (HIP 47791) with an age of 1.4 Gyr. Fragmentation is proposed as the most probable formation process for the eight systems. A comparison between the estimated masses and the dynamical ones lead to new dynamical parallaxes for four systems: (28.63 ± 0.56) mas for HIP 14524, (15.6 ± 0.63) mas for HIP 16025, (9.73 ± 0.26) mas for HIP 47791, and (16.53 ± 0.59) mas for HIP 73085. Hence, the orbital solutions were reclassified. We conclude that Gaia DR3 parallaxes are more precise than those given by Gaia DR2 and Hipparcos 2.