bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of Functional Properties of Sri Lankan Ambarella (Spondias dulcis Forst. syn. Spondias cytherea Sonn.) Fruit and Development of Vacuum Dried Ambarella Fruit Powder and Incorporated Soup Mix
Authors: Navoda N. P. G. D. , Samaranayake M. D. W. , Liyanage S. L. , Herath H. M. T. , Jayasinghe J. M. J. K. 
Published: January 2022

Objective: To develop vacuum dried (VD) Ambarella fruit powder and Ambarella fruit incorporated soup mix from large and miniature Ambarella and investigation of antioxidants and anti-diabetic properties.
Methods: Ethanolic extracts of two Ambarella varieties were analyzed for antioxidant potential in terms of total phenolic content (TPC), total ﬂavonoid content (TFC), ferrous reducing antioxidant power (FRAP), radical scavenging activities of DPPH and ABTS and α-amylase inhibitory activity.
Results: Fresh dwarf Ambarella showed a significantly (p<0.05) high TPC (3.35±0.10 mgGAE/g) while fresh large Ambarella showed a significantly (p<0.05) high FRAP (0.71±0.13 mgTE/g) and DPPH (3.57±0.31 mg TE/g). In comparison of ethanolic extracts of VD Ambarella powders, a significantly (p<0.05) higher antioxidant potential in terms of FRAP (4.19±0.06 mgTE/g) exhibited in dwarf variety while significantly (p<0.05) higher ABTS (4.03±0.27 mgTE/g) and DPPH (3.00±0.49mgTE/g) exhibited in large Ambarella. Alpha amylase inhibition activities of fresh fruits of large and dwarf were 46.30±4.07% and 49.55±3.18% where as in VD powders were 27.59±5.03% and 15.58±5.86% respectively. An instant soup mixture was developed incorporating 20% of VD powder from large variety due its abundance. The antioxidant potential of ethanolic extract of the soup mixture in terms of TPC, TFC, ABTS, and FRAP were 0.55±0.00 mgGAE/g, 0.04±0.00 mgQE/g, 1.65±0.06 mgTE/g, and 0.04±0.15 mgTE/g. Alpha amylase inhibition activity of soup mixture was 39.49±0.29%.
Conclusion: The both types of fresh fruits exhibited higher antioxidant potential except FRAP and higher anti-amylase inhibition than VD Ambarella powder.
Purification and Characterization of Superoxide Dismutase Enzyme from Punica granatum L.
Authors: Ozdemir Caglar , Gulesci Nuri , Bilgin Ramazan 
Published: January 2022

Superoxide dismutase (SOD) constitutes a very important antioxidant defense against oxidative stress in the body. SOD is found aplenty in many organisms, from microorganisms to plants and animals. Pomegranate (Punica granatum L.) peels and fruits have been used in traditional medicine. In this study, SOD (E.C 1.15.1.1) was purified from Punica granatum L. For this purpose, Punica granatum L. was homogenized centrifuged, fractioned with ammonium sulfate to precipitate, and then DEAE chromatography separation was applied. Punica granatum L. was purified 16.60-fold. SOD enzyme-specific activity was found as 166 U/mg protein.
Spray‐n‐Sense: Sprayable Nanofibers for On‐Site Chemical Sensing
Authors: Bakshi Shrishty , Snoswell Aaron J. , Kwok Kei Yeung , Cheng Lung Hei , Altalhi Tariq A. , Santos Abel , Popat Amirali , Kumeria Tushar 
Published: January 2022

AbstractA versatile “Spray‐n‐Sense” sprayable nanofiber technology for on‐site chemical detection is demonstrated. Driven by compressed gas, the “Spray‐n‐Sense” nanofibers, as the name suggests, can be directly sprayed onto any kind or shape of surfaces, while the embedded chemical reporter enables simple colorimetric/fluorometric detection. Herein, nanofibers are sprayed on several surfaces including cardboard, glass, plastic, and rubber. The sensing capabilities of the “Spray‐n‐Sense” nanofibers are established through the detection of three different analytes including two metal ions (Fe2+ and Fe3+ using 1,10‐phenanthroline and curcumin as chromogenic reporters, respectively) and ammonia (using rhodamine‐B as a fluorogenic reporter) in aqueous media. Additional to being highly portable, the “Spray‐n‐Sense” nanofibers show impressive sensor characteristics with a sub‐ppm lower limit of detection (LLOD) for Fe2+ and a wide linear working range. Whereas, the LLOD of 6.2 and 32 ppm is achieved for Fe3+ ions and ammonia, respectively. A custom‐designed smartphone application enables quantitative analysis for reliable on‐site sensing. The selectivity and specificity are imparted by the embedded reporter, and thus allow analyte detection in complex real‐life samples and simultaneous detection of multiple chemical species through co‐doping. The “Spray‐n‐Sense” nanofibers also allow for the detection of target analytes in solid samples.
Stoichiometry‐Controlled Phase Engineering of Cesium Bismuth Halides and Reversible Structure Switch
Authors: Yan Jun , Zhang Shuai , Wei Qilin , Cao Sheng , Zhao Jialong , Zou Bingsuo , Zeng Ruosheng 
Published: January 2022

AbstractPerovskite metal halides have attracted extensive attention because of their excellent photoelectric properties and structural adjustability. Herein, the authors report a facile method for the controlled synthesis of cesium‐bismuth halides by controlling the stoichiometry of Cs/Bi precursors at room temperature. The 0D Cs3BiCl6 and 1D Cs3Bi2Cl9 can be obtained at the Cs/Bi feed ratio of 3:1 and 3:2, respectively. Though both metal halides exhibit a very low photoluminescence quantum yield (PLQY) due to their intrinsic nature of indirect band gaps, they exhibited bright orange broadband emission after Mn2+ doping. Combining density functional theory (DFT) calculations, it is found that an appropriate amount of Mn2+ doping does not change the crystal structures of cesium‐bismuth halides, but Mn 3d orbitals produce impurity states in the forbidden energy gaps of the host structures, resulting in efficient energy transfer from the conduction band of the host to d‐state of Mn ions. Importantly, the reversible structural switch of Mn‐doped Cs3BiCl6 and Cs3Bi2Cl9 metal halides can be flexibly adjusted through the post addition of the CsCl/BiCl3 precursors. These results tremendously enrich the synthetic chemistry of low‐dimensional metal halides and provide a beneficial reference for the regulation of crystal structure and optical properties of metal halides.
Phosphorylated and Phosphonated Low‐Complexity Protein Segments for Biomimetic Mineralization and Repair of Tooth Enamel
Authors: Chang Rong , Liu Yang‐Jia , Zhang Yun‐Lai , Zhang Shi‐Ying , Han Bei‐Bei , Chen Feng , Chen Yong‐Xiang 
Published: January 2022

AbstractBiomimetic mineralization based on self‐assembly has made great progress, providing bottom‐up strategies for the construction of new organic–inorganic hybrid materials applied in the treatment of hard tissue defects. Herein, inspired by the cooperative effects of key components in biomineralization microenvironments, a new type of biocompatible peptide scaffold based on flexibly self‐assembling low‐complexity protein segments (LCPSs) containing phosphate or phosphonate groups is developed. These LCPSs can retard the transformation of amorphous calcium phosphate into hydroxyapatite (HAP), leading to merged mineralization structures. Moreover, the application of phosphonated LCPS over phosphorylated LCPS can prevent hydrolysis by phosphatases that are enriched in extracellular mineralization microenvironments. After being coated on the etched tooth enamel, these LCPSs facilitate the growth of HAP to generate new enamel layers comparable to the natural layers and mitigate the adhesion of Streptococcus mutans. In addition, they can effectively stimulate the differentiation pathways of osteoblasts. These results shed light on the potential biomedical applications of two LCPSs in hard tissue repair.
Unveiling the Emergent Traits of Chiral Spin Textures in Magnetic Multilayers
Authors: Chen Xiaoye , Lin Ming , Kong Jian Feng , Tan Hui Ru , Tan Anthony K.C. , Je Soong‐Geun , Tan Hang Khume , Khoo Khoong Hong , Im Mi‐Young , Soumyanarayanan Anjan 
Published: January 2022

AbstractMagnetic skyrmions are topologically wound nanoscale textures of spins whose ambient stability and electrical manipulation in multilayer films have led to an explosion of research activities. While past efforts focused predominantly on isolated skyrmions, recently ensembles of chiral spin textures, consisting of skyrmions and magnetic stripes, are shown to possess rich interactions with potential for device applications. However, several fundamental aspects of chiral spin texture phenomenology remain to be elucidated, including their domain wall (DW) structure, thermodynamic stability, and morphological transitions. Here the evolution of these textural characteristics are unveiled on a tunable multilayer platform—wherein chiral interactions governing spin texture energetics can be widely varied—using a combination of full‐field electron and soft X‐ray microscopies with numerical simulations. With increasing chiral interactions, the emergence of Néel helicity, followed by a marked reduction in domain compressibility, and finally a transformation in the skyrmion formation mechanism are demonstrated. Together with an analytical model, these experiments establish a comprehensive microscopic framework for investigating and tailoring chiral spin texture character in multilayer films.
Sandwiching Phosphorene with Iron Porphyrin Monolayer for High Stability and Its Biomimetic Sensor to Sensitively Detect Living Cell Released NO
Authors: Zhang Chunmei , Hu Fangxin , Hao Xijuan , Rao Qianghai , Hu Tao , Sun Wei , Guo Chunxian , Li Chang Ming 
Published: January 2022

AbstractInstability of 2D phosphorene material is the major obstacle for its broad applications. Herein phosphorene is sandwiched with self‐assembled iron porphyrin monolayers on both sides (I‐Phene) to significantly enhance stability. Iron porphyrin has strong interaction with phosphorene through formation of PFe bonds. The sandwich structure offers excellent stability of phosphorene by both‐sided monolayer protections for an intact phosphorene structure more than 40 days under ambient conditions. Meanwhile, the electron transfer between iron porphyrin and phosphorene result in a high oxidation state of Fe, making I‐Phene biomimetic sensitivity toward oxidation of nitric oxide (NO) for 2.5 and 4.0 times higher than phosphorene and iron‐porphyrin alone, respectively. Moreover, I‐Phene exhibits excellent selectivity, a wide detection range, and a low detection limit at a low oxidation potential of 0.82 V, which is comparable with the reported noble metal based biomimetic sensors while ranking the best among all non‐noble biomimetic ones. I‐Phene is further used for real‐time monitoring NO released from cells. This work provides effective approach against phosphorene degrading for outstanding stability, which has universal significance for its various important applications, and holds a great promise for a highly sensitive biomimetic sensor in live‐cell assays.
Agitation of yield stress fluids by gas injection
Authors: Hojeij Ali , Jossic Laurent , Séchet Philippe , Magnin Albert , Hattou Stéphane 
Published: January 2022

AbstractThis study is in line with two previous studies by the same authors on gas injection in yield stress fluids. Gas is injected toward the bottom wall of a prismatic tank containing a yield stress fluid. When rising toward the free surface, trains of bubbles generate fluid recirculation in the tank. Two experimental colorimetric methods are introduced and validated in order to quantify the recirculation liquid flow rate as well as the time evolution of the extent and shape of the mixed volume. The influences of the injection flow rate, fluid rheology, and reactor size have been quantified. Correlations based on the characteristic nondimensional numbers of the flow have been developed to predict the downward liquid flow rate as well as the mixed volume. A model for estimating the mixing time is also developed and compared to experimental results.
Impact of achieving a complete response to initial therapy of multiple myeloma and predictors of subsequent outcome
Authors: Kaddoura Marcella , Binder Moritz , Dingli David , Buadi Francis K. , Lacy Martha Q. , Gertz Morie A. , Dispenzieri Angela , Kapoor Prashant , Hwa Lisa , Fonder Amie , Hobbs Miriam , Hayman Suzanne , Leung Nelson , Go Ronald S. , Lin Yi , Gonsalves Wilson , Kourelis Taxiarchis , Warsame Rahma , Kyle Robert A. , Rajkumar Vincent , Kumar Shaji 
Published: January 2022

AbstractAchievement of a complete response (CR) in multiple myeloma (MM) correlates with improvement in survival outcomes; however, its impact on prognostic variables at baseline outside of clinical trial settings is not well described. We sought to determine the impact of achieving a CR within 2 years from diagnosis, its effect on the prognostic value of fluorescence in situ hybridization (FISH) and International Staging System (ISS) risk, and examined additional predictors of outcome among those achieving a CR in a routine clinical setting. We evaluated 1869 newly diagnosed MM patients who had ≥ 2 monoclonal protein immunofixation studies in the serum and urine available within 24 months from diagnosis, categorizing those with ≥ 2 negative serum and urine immunofixations as achieving CR. With a landmark at 24 months, median progression‐free survival (PFS) for CR versus non‐CR patients was 29.8 versus 20.9 months (p ≤ .0002); median overall survival (OS) was 104 versus 70 months (p < .0001). The impact of achieving a CR was retained after adjusting for FISH, ISS, sex, transplant status, and involved heavy chain. Baseline FISH and ISS stage were not associated with PFS or OS among patients achieving a CR. The following variables were found as predictors of inferior OS within the CR cohort: age > 75 years, male gender, hypoalbuminemia, and non‐immunoglobulin G involved heavy chain. Our study confirms that achievement of CR within 2 years from diagnosis is associated with improvement in survival outcomes and neutralization of the impact of FISH and ISS risk, thereby confirming observations from the clinical trial setting among a clinical practice cohort.
Cytogenetic risk classification maintains its prognostic significance in transplanted FLT3‐ITD mutated acute myeloid leukemia patients: On behalf of the acute leukemia working party/European society of blood and marrow transplantation
Authors: Nagler Arnon , Labopin Myriam , Craddock Charles , Socié Gerard , Yakoub‐Agha Ibrahim , Gedde‐Dahl Tobias , Niittyvuopio Riitta , Byrne Jennifer Louise , Cornelissen Jan J. , Labussière‐Wallet Hélène , Arcese William , Milpied Noel , Esteve Jordi , Canaani Jonathan , Mohty Mohamad 
Published: January 2022

AbstractFMS‐like tyrosine kinase 3 internal tandem duplication (FLT3‐ITD) mutational status is a pivotal prognosticator in acute myeloid leukemia (AML) patients and significantly increases the risk of disease relapse. However, it remains unclear whether in FLT3‐ITD patients referred for allogeneic stem cell transplantation (allo‐SCT), baseline cytogenetics significantly impacts clinical outcome. Using the European Society of Blood and Marrow Transplantation registry, we performed a retrospective analysis of 1631 FLT3‐ITD AML patients who underwent allo‐SCT with the aim of determining the influence of cytogenetic risk category on patient outcomes. Median patient age was 49 years and median follow‐up duration was 36 months. Two‐year leukemia‐free survival (LFS) and incidence of relapse were 54% and 31.6%, respectively. Non‐relapse mortality was experienced by 14.4% with a 2‐year overall survival (OS) of 60.1%. On multivariate analysis, LFS was significantly lower in patients with intermediate and adverse risk cytogenetics compared with those with favorable risk cytogenetics, (hazard ratio [HR] = 1.48, 95% confidence interval [CI], 1.06–2.06; p = .02), and (HR = 01.65, 95% CI, 1.13–2.40; p = .009), respectively. OS was significantly lower in patients with adverse risk cytogenetics compared with patients with favorable risk cytogenetics (HR = 1.74, 95% CI, 1.16–2.61; p = .008) with a trend toward lower OS in patients with intermediate risk cytogenetics compared to those with favorable risk cytogenetics (HR = 1.43, 95% CI, 1.00–2.05; p = .052). In addition, adverse risk patients and intermediate risk patients experienced higher relapse rates compared with favorable risk patients (HR = 1.83, 95% CI, 1.13–2.94; p = .013 and HR = 1.82, 95% CI, 1.19–2.77; p = .005). Overall, cytogenetic studies aid in refinement of risk stratification in transplanted FLT3‐ITD AML patients.
Accumulation of high molecular weight kininogen in the brains of Alzheimer's disease patients may affect microglial function by altering phagocytosis and lysosomal cathepsin activity
Authors: Zamolodchikov Daria , Duffield Michael , Macdonald Lynn E. , Alessandri‐Haber Nicole 
Published: January 2022

AbstractIncreased activation of the contact system protein high molecular weight kininogen (HK) has been shown in plasma and cerebrospinal fluid of Alzheimer's disease (AD) patients, but its potential role in the brain has not been explored. We assessed HK levels in brain tissue from 20 AD patients and controls and modeled the effects of HK on microglia‐like cells in culture. We show increased levels of HK in the hippocampus of AD patients, which colocalized with amyloid beta (Aβ) deposits and activated microglia. Treatment of microglia with HK led to cell clustering and elevated levels of phagocytosed Aβ. We demonstrate that microglia internalize HK and traffic it to lysosomes, which is accompanied by reduced activity of lysosomal cathepsins L and S. Our results suggest that HK accumulation in the AD hippocampus may alter microglial uptake and degradation of Aβ fibrils, possibly contributing to microglial dysfunction in AD.
Preserving Brain LPC‐DHA by Plasma Supplementation Attenuates Brain Injury after Cardiac Arrest
Authors: Nishikimi Mitsuaki , Shoaib Muhammad , Choudhary Rishabh C. , Aoki Tomoaki , Miyara Santiago J. , Yagi Tsukasa , Hayashida Kei , Takegawa Ryosuke , Yin Tai , Becker Lance B. , Kim Junhwan 
Published: January 2022

ObjectiveCardiac arrest (CA) is a major health burden with brain damage being a significant contributor to mortality. We found lysophosphatidylcholine (LPC), including a species containing docosahexaenoic acid (LPC‐DHA), was significantly decreased in plasma post‐CA, supplementation of which significantly improved neurological outcomes. The aim of this study is to understand the protective role of LPC‐DHA supplementation on the brain post‐CA.MethodsWe first evaluated associations between the plasma level of LPC‐DHA and neurological injury and outcomes of human patients with CA. We then utilized a rat CA model and cell cultures to investigate therapeutic and mechanistic aspects of plasma LPC‐DHA supplementation.ResultsWe found that decreased plasma LPC‐DHA was strongly associated with neurological outcomes and disappearance of the difference between gray and white matter in the brain after CA in human patients. In rats, the decreased plasma LPC‐DHA was associated with decreased levels of brain LPC‐DHA after CA, and supplementing plasma LPC‐DHA normalized brain levels of LPC‐DHA and alleviated neuronal cell death, activation of astrocytes, and expression of various inflammatory and mitochondrial dynamics genes. We also observed deceased severity of metabolic alterations with LPC‐DHA supplementation using untargeted metabolomics analysis. Furthermore, LPC treatment showed a similar protective effect for neurons and astrocytes in mixed primary brain cell cultures.InterpretationThe observed neuroprotection accompanied with normalized brain LPC‐DHA level by plasma supplementation implicate the importance of preventing the decrease of brain LPC‐DHA post‐CA for attenuating brain injury. Furthermore, the data supports the causative role of decreased plasma LPC‐DHA for brain damage after CA. ANN NEUROL 2022;91:389–403
Exceptional Manganese(II) Stability and Manganese(II)/Zinc(II) Selectivity with Rigid Polydentate Ligands**
Authors: Cieslik Patrick , Comba Peter , Dittmar Benedikt , Ndiaye Daouda , Tóth Éva , Velmurugan Gunasekaran , Wadepohl Hubert 
Published: January 2022

AbstractWhile MnII complexes meet increasing interest in biomedical applications, ligands are lacking that enable high MnII complex stability and selectivity vs. ZnII, the most relevant biological competitor. We report here two new bispidine derivatives, which provide rigid and large coordination cavities that perfectly match the size of MnII, yielding eight‐coordinate MnII complexes with record stabilities. In contrast, the smaller ZnII ion cannot accommodate all ligand donors, resulting in highly strained and less stable six‐coordinate complexes. Combined theoretical and experimental data (X‐ray crystallography, potentiometry, relaxometry and 1H NMR spectroscopy) demonstrate unprecedented selectivity for MnII vs. ZnII (KMnL/KZnL of 108–1010), in sharp contrast to the usual Irving–Williams behavior, and record MnII complex stabilities and inertness with logKMnL close to 25.
A new slit‐radial die for simultaneously measuring steady state shear viscosity and first normal stress difference of viscoelastic liquids via capillary rheometry
Authors: Khabazian Esfahani Masood , Georgantopoulos Christos K. , Naue Ingo F. C. , Sunder Joachim , Wilhelm Manfred 
Published: January 2022

AbstractA new slit‐radial die capable of simultaneously obtaining steady state shear viscosity and the average first normal stress difference coefficient via capillary rheometry has been developed. The steady state shear viscosity and average first normal stress difference coefficient are calculated in the slit part and radial part of the die, respectively. The steady state shear viscosity from the slit part of the slit‐radial die is compared to shear viscosities obtained from a capillary die and also the magnitude of the complex viscosity obtained from oscillatory shear experiment. The average value of first normal stress difference coefficient which is calculated in the radial part of the slit‐radial die is compared to first normal stress difference coefficient obtained from transient shear experiment in a cone‐plate geometry and the molecular stress function model predictions. The effect of variation of power law fitting parameters (consistency index, k and power law index, n) on average value of the first normal stress difference coefficient obtained from the radial part of the slit‐radial die is discussed. As this die has the shape of the city map of Karlsruhe it is named as Karlsruhe die.