bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diapause characterization in the invasive alien mosquito species Aedes koreicus: a laboratory experiment
Authors: Marini Giovanni , Arnoldi Daniele , Inama Enrico , Rizzoli Annapaola 
Published: September 2022

Abstract
Aedes koreicus is an invasive alien mosquito species native to Asia now introduced in several European countries, including northern Italy. In this temperate region, mosquito populations survive cold winter temperatures thanks to diapausing eggs or adults, depending on the species. In its native area, Ae. koreicus was reported to overwinter in the egg stage, but to the best of our knowledge, it is not confirmed whether overwintering eggs are actually diapausing or only in a quiescence stage, i.e., they might hatch as soon as external conditions are favorable. Based on previous laboratory studies, we established a diapausing Ae. koreicus colony, maintained at 21 °C with a photoperiod of 12L:12D. Females were allowed to lay eggs, which were consequently placed in water at different time intervals after oviposition, from 30 days to 5 months. We found that diapausing eggs younger than 3 months have a poor hatching rate, while after about 100 days we observed that almost all eggs hatched. Our findings highlight that water immersion alone did not lead to the hatching of eggs, as age was found to be a significantly important factor. We thus confirm effective diapause, occurring at the egg stage, for Ae. koreicus in a recently invaded area. Moreover, our quantification of diapause duration and hatching success might help in better designing future experiments and improving modeling efforts.

Graphical Abstract
CircPRKCH modulates extracellular matrix formation and metabolism by regulating the miR-145/HGF axis in osteoarthritis
Authors: Que Wenzhong , Liu Huili , Yang Qinqin 
Published: September 2022

Abstract
Background
Osteoarthritis (OA) is a chronic degenerative joint disease. Extracellular matrix (ECM) degradation is essential for OA progression. Previous studies have shown that circular RNAs (circRNAs) are involved in the pathological process of OA. CircPRKCH has been shown to be upregulated in OA chondrocytes. The present study was aimed to explore the roles of circPRKCH in vivo and in vitro models of OA and its underlying molecular mechanisms.

Methods
IL-1β-induced chondrocytes and mice injected with monosodium iodoacetate were used as OA models in vitro and in vivo, respectively. RT-qPCR was performed to measure the expression of circPRKCH, miR-145, and HGF in cartilage tissues and chondrocytes. The interaction between miR-145 and circPRKCH or HGF was verified by a dual-luciferase reporter assay. Chondrocyte apoptosis, viability, and ECM-related proteins were examined by flow cytometry, MTT assay, and Western blotting, respectively. Histopathological changes were detected by HE and Safranin O-fast green staining.

Results
The expression of circPRKCH and HGF was increased in OA cartilage tissues and IL-1β-treated chondrocytes, while miR-145 expression was decreased. IL-1β induced chondrocyte apoptosis and ECM degradation in chondrocytes. Moreover, circPRKCH promoted HGF expression and activated HGF/c-MET by directly binding to miR-145. miR-145 knockdown or HGF overexpression significantly reversed circPRKCH knockdown-mediated inhibition of apoptosis and ECM degradation in IL-1β-induced chondrocytes. Besides, miR-145 overexpression alleviated IL-1β-induced chondrocyte apoptosis and ECM degradation by inhibiting HGF/c-MET. Finally, circPRKCH knockdown reduced ECM degradation by regulating the miR-145/HGF axis in an experimental OA model in mice.

Conclusion
Our study demonstrated that circPRKCH promoted chondrocyte apoptosis and ECM degradation via the miR-145/HGF axis in OA, which may provide a novel target for OA treatment.
Prognostic factors for the development of systemic lupus erythematosus in patients with immune thrombocytopenia
Authors: Ahn Soo Min , Choi Eun-Ji , Oh Ji Seon , Kim Yong-Gil , Lee Chang-Keun , Yoo Bin , Hong Seokchan 
Published: September 2022

AbstractBackgroundPatients with immune thrombocytopenia (ITP) have a risk of developing systemic lupus erythematosus (SLE). We sought to examine the clinical characteristics of patients with primary ITP who later developed SLE and identified the risk factors for the development of SLE.MethodsWe retrospectively examined patients who were diagnosed with primary ITP at a tertiary hospital between August 2001 and November 2019. We compared the clinical characteristics according to the development of SLE. Logistic regression analysis was performed to identify the factors associated with the development of SLE.ResultsOf 130 patients with primary ITP, 10 (7.7%) were later diagnosed with SLE during follow-up (median, 30 months [IQR, 15.5–105]). The presence of skin bleeding, organ bleeding, lymphocytopenia, anemia, and antinuclear antibody (ANA) positivity (≥ 1:160) were more common among patients who later developed SLE than did those who did not develop SLE. Multivariate analysis showed that young age (< 40 years; odds ratio [OR], 6.307 [95% confidence interval (CI), 1.114–34.908];P = 0.035), organ bleeding (OR, 13.672 [95% CI, 2.437–76.689];P = 0.003), and ANA positivity (1:160; OR, 6.638 [95% CI, 1.399–31.504];P = 0.017) were significantly associated with the development of SLE.ConclusionsYoung age (< 40 years), organ bleeding, and ANA positivity (≥ 1:160) were risk factors for the development of SLE in patients with primary ITP. Close follow-up is needed to detect the development of SLE in patients with ITP and the abovementioned risk factors.
Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis
Authors: Wu Zuping , He Yuying , Chen Sirui , Zhu Li , Wang Jiahe , Zhang Demao , Xie Jing , Zou Shujuan , Zhou Chenchen 
Published: September 2022

AbstractBackgroundOrthodontic tooth movement inevitably induces cementum resorption, which is an urgent problem for orthodontists to confront. Human periodontal ligament stem cells (hPDLSCs) exert an important role in the orthodontic tooth movement and exhibit multidirectional differentiation ability in cementum regeneration. Connective tissue growth factor (CTGF) is an important extracellular matrix protein for bone homeostasis and cell differentiation. The purpose of our study was to explore the role of CTGF in cementum repair and cementogenesis and to elucidate its underlying mechanism.MethodsA cementum defect model was established by tooth movement with heavy forces, and the cementum repair effect of CTGF was observed via micro-CT, HE staining and immunohistochemical staining. RT‒qPCR, western blotting (WB), alizarin red staining and ALP activity experiments verified the mineralization ability of hPDLSCs stimulated with CTGF. The expression of Cx43 in periodontal ligament cells was detected by WB and immunofluorescence (IF) experiments after CTGF stimulation in vivo and in vitro. Subsequently, the mineralization ability of hPDLSCs was observed after application of CTGF and the small interfering RNA Si-Cx43. Additionally, co-intervention via application of the small interfering RNA Si-CTGF and the Cx43 agonist ATRA in hPDLSCs was performed to deepen the mechanistic study. Next, WB, IF experiments and co-immunoprecipitation were conducted to confirm whether CTGF triggers the Cx43/β-catenin axis to regulate cementoblast differentiation of hPDLSCs.ResultsLocal oral administration of CTGF to the cementum defects in vivo facilitated cementum repair. CTGF facilitated the cementogenesis of hPDLSCs in a concentration-dependent manner. Cx43 acted as a downstream effector of CTGF to regulate cementoblast differentiation. Si-Cx43 reduced CTGF-induced cementoblast differentiation. The Cx43 agonist ATRA restored the low differentiation capacity induced by Si-CTGF. Further mechanistic studies showed that CTGF triggered the activation of β-catenin in a dose-dependent manner. In addition, co-localization IF analysis and co-immunoprecipitation demonstrated that Cx43 interacted with β-catenin at cell‒cell connections. Si-Cx43 attenuated the substantial expression of β-catenin induced by CTGF. The Cx43 agonist reversed the inhibition of β-catenin induced by Si-CTGF. IF demonstrated that the nuclear importation of β-catenin was related to the immense expression of Cx43 at cell‒cell junctions.ConclusionsTaken together, these data demonstrate that CTGF promotes cementum repair and cementogenesis through activation of the Cx43/β-catenin signalling axis.
Silent witnesses: the experience of having a sibling with anorexia nervosa
Authors: Hutchison Suzanne , House Jennifer , McDermott Beth , Simic Mima , Baudinet Julian , Eisler Ivan 
Published: September 2022

Abstract
Background
This study explored the experience of having a sibling with anorexia nervosa and the sibling perspectives on service provision.

Method
Four focus groups were conducted with 14 siblings (8 female, 6 male, age 11–19 years) of adolescents with anorexia nervosa or related restrictive eating disorders. Group discussions were transcribed and analysed using thematic analysis.

Results
Four themes and eight sub-themes were generated. These illustrated siblings feel greatly affected by the way the family needs to change to support someone with anorexia nervosa. Feelings of ambivalence and acceptance were also evident. They described silencing their own emotions and needs so as not to trouble others, and distancing themselves from their families in order to cope. Some female (but no male) siblings identified an impact on their own perceptions of eating and body image. Siblings generally felt that services had not attended to their needs, and that they had not been appropriately included in treatment.

Conclusions
Data from this study suggest the sibling experience needs to be more carefully considered and included in treatment. This may include a more explicit invitation to sessions and a more active discussion about their own needs and useful involvement in treatment sessions. Findings point to ways siblings may be better supported, such as peer support groups.
Prediction of mortality and morbidity following paraquat poisoning based on trend of liver and kidney injury
Authors: Gheshlaghi Farzad , Haghirzavareh Jamileh , Wong Anselm , Golshiri Parastoo , Gheshlaghi Shayan , Eizadi-Mood Nastaran 
Published: September 2022

Abstract
Background
Paraquat is a non-selective herbicide that causes severe tissue damage in various organs including the liver and kidney. The aim of this study was to determine the trend of the liver and kidney injury in patients with paraquat poisoning.

Methods
This retrospective cross-sectional study was performed at the Khorshid Hospital referral poisoning emergency center. The medical records of all patients with acute paraquat poisoning admitted from March 2017 to October 2020 were reviewed. Demographic factors, liver and kidney function tests and outcomes were recorded. Patients were divided into two groups based on the outcome of mortality (death or survived). The two groups were compared in terms of changes in creatinine and liver enzymes during hospitalization.

Results
A significant difference in mean creatinine levels between the two groups was observed from the third day after admission. The peak median Cr was 3.5 mg/dl for deceased patients in day 6 and 1.47 mg/dl for survived patients on 4th day. Minor elevations of ALT and AST were present in those who died. Logistic regression analysis shows patients who had level of creatinine higher than normal from the 2nd to 6th day post overdose, the risk of mortality was 4.83 to 7.44 times more than patients with normal creatinine level. The mean (SD) area under the curve for outcome prediction was reported to be excellent for creatinine on the 8th day post overdose (85.7 ± 13.2). Creatinine was higher than 2 on the 8th day post ingestion and had a sensitivity 100% and specificity 85.7% for mortality prediction (P value, 0.05).

Conclusions
The risk of mortality secondary to paraquat ingestion was highly associated with a rise in creatinine. Minor elevations of ALT and AST were also present in those who died. The creatinine concentration on different days post overdose can be helpful in predicting the severity of poisoning especially when the serum paraquat levels are not available.
Needs assessment and planning for a clinic-community-based implementation program for hypertension control among blacks in New York City: a protocol paper
Authors: Gyamfi Joyce , Cooper Claire , Barber Aigna , Onakomaiya Deborah , Lee Wen-Yu , Zanowiak Jennifer , Mansu Moses , Diaz Laura , Thompson Linda , Abrams Roger , Schoenthaler Antoinette , Islam Nadia , Ogedegbe Gbenga 
Published: September 2022

Abstract
Background
Hypertension (HTN) control among Blacks in the USA has become a major public health challenge. Barriers to HTN control exist at multiple levels including patient, physician, and the health system. Patients also encounter significant community-level barriers, such as poor linkage to social services that impact health (unstable housing, food access, transportation). We describe a multi-component needs assessment to inform the development, implementation, and evaluation of a program to improve HTN management within a large healthcare system in New York City (NYC).

Methods
Guided by the Community-Based Participatory Research (CBPR) and Consolidated Framework for Implementation Research (CFIR) frameworks, data will be collected from four main sources: (1) quantitative surveys with health systems leadership, providers, and staff and with community-based organizations (CBOs) and faith-based organizations (FBOs); (2) qualitative interviews and focus groups with health systems leadership, providers, and staff and with CBOs and FBOs; (3) NYC Community Health Survey (CHS); and (4) New York University (NYU) Health system Epic Electronic Health Record (EHR) system. The data sources will allow for triangulation and synthesis of findings.

Discussion
Findings from this comprehensive needs assessment will inform the development of a clinic-community-based practice facilitation program utilizing three multi-level evidence-based interventions (nurse case management, remote blood pressure (BP) monitoring, and social determinants of health (SDoH) support) integrated as a community-clinic linkage model for improved HTN control in Black patients. Integration of stakeholders’ priorities, perspectives, and practices into the development of the program will improve adoption, sustainability, and the potential for scale-up.

Trial registration
NCT05208450; registered on January 26, 2022
From compressive sampling to compressive tasking: retrieving semantics in compressed domain with low bandwidth
Authors: Zhang Zhihong , Zhang Bo , Yuan Xin , Zheng Siming , Su Xiongfei , Suo Jinli , Brady David J. , Dai Qionghai 
Published: September 2022

AbstractHigh-throughput imaging is highly desirable in intelligent analysis of computer vision tasks. In conventional design, throughput is limited by the separation between physical image capture and digital post processing. Computational imaging increases throughput by mixing analog and digital processing through the image capture pipeline. Yet, recent advances of computational imaging focus on the “compressive sampling”, this precludes the wide applications in practical tasks. This paper presents a systematic analysis of the next step for computational imaging built on snapshot compressive imaging (SCI) and semantic computer vision (SCV) tasks, which have independently emerged over the past decade as basic computational imaging platforms.SCI is a physical layer process that maximizes information capacity per sample while minimizing system size, power and cost. SCV is an abstraction layer process that analyzes image data as objects and features, rather than simple pixel maps. In current practice, SCI and SCV are independent and sequential. This concatenated pipeline results in the following problems:i) a large amount of resources are spent on task-irrelevant computation and transmission,ii) the sampling and design efficiency of SCI is attenuated, andiii) the final performance of SCV is limited by the reconstruction errors of SCI. Bearing these concerns in mind, this paper takes one step further aiming to bridge the gap between SCI and SCV to take full advantage of both approaches.After reviewing the current status of SCI, we propose a novel joint framework by conducting SCV on raw measurements captured by SCI to select the region of interest, and then perform reconstruction on these regions to speed up processing time. We use our recently built SCI prototype to verify the framework. Preliminary results are presented and the prospects for a joint SCI and SCV regime are discussed. By conducting computer vision tasks in the compressed domain, we envision that a new era of snapshot compressive imaging with limited end-to-end bandwidth is coming.
R2 gravity effects on the kinetic axion phase space
Authors: Oikonomou V. K. 
Published: September 2022

Abstract
In this work we consider the effect of an R
2 term on the kinetic misalignment axion theory. By using the slow-roll assumptions during inflation and the field equations, we construct an autonomous dynamical system for the kinetic axion, including the effects of the R
2 term and we solve numerically the dynamical system. As we demonstrate, the pure kinetic axion attractor is transposed to the right in the field phase space, and it is no longer

, but it is

, with

some non-zero value of the scalar field with

. This feature indicates that the kinetic axion mechanism is enhanced, and the axion oscillations are further delayed, compared with the pure kinetic axion case. The phenomenological implications on the duration of the inflationary era, on the commencing of the reheating era and the reheating temperature, are also discussed.
Long-Term Exposure to Neighborhood Policing and the Racial/Ethnic Gap in High School Graduation
Authors: Legewie Joscha , Cricco Nino José 
Published: September 2022

Abstract
Researchers are increasingly exploring the consequences of policing for the educational outcomes of minority youth. This study contributes to this literature by asking three questions. First, what are racial/ethnic disparities in long-term exposure to neighborhood policing? Second, how does this exposure affect high school graduation? Third, how much of the ethnoracial gap in high school graduation would remain if neighborhood policing was equalized? To address these questions, we use data from the New York City Department of Education and follow five cohorts of NYC public school students from middle to high school. Our findings reveal starkly different experiences with neighborhood policing across racial/ethnic groups. Using novel methods for time-varying treatment effects, we find that long-term exposure to neighborhood policing has negative effects on high school graduation, with important differences across racial/ethnic groups. Using gap-closing estimands, we show that assigning a sample of Black and Latino students to the same level of neighborhood policing as White students would close the Black–White gap in high school graduation by more than one quarter and the Latino–White gap by almost one fifth. Alternatively, we explore interventions where policing is solely a function of violent crime, which close the Black–White gap by as much as one tenth. Our study advances previous research by focusing on cumulative, long-term exposure to neighborhood policing and by assessing various counterfactual scenarios that inform research and policy.
Desirable attributes of theories, models, and frameworks for implementation strategy design in healthcare: a scoping review protocol
Authors: Porat-Dahlerbruch Joshua , Fontaine Guillaume , Bourbeau-Allard Ève , Spinewine Anne , Grimshaw Jeremy M. , Ellen Moriah E. 
Published: September 2022

Background: Implementation strategies can facilitate the adoption of evidence-based practices and policies. A wide range of theoretical approaches—theories, models, and frameworks—can be used to inform implementation strategy design in different ways (e.g., guiding barrier and enabler assessment to implementing evidence-based interventions). While selection criteria and attributes of theoretical approaches for use in implementation strategy design have been studied, they have never been synthesized. Furthermore, theoretical approaches have never been classified according to desirable criteria and attributes for use in implementation strategy design. This scoping review aims to a) identify the literature reporting on the selection of theoretical approaches for informing implementation strategy design in healthcare and b) understand the suggested use of these approaches in implementation strategy design. Methods: The Joanna Briggs Institute methodological guidelines will be used to conduct this scoping review. A search of three bibliographical databases (MEDLINE, Embase, CINAHL) will be conducted for peer-reviewed discussion, methods, protocol, or review papers. Data will be managed using the Covidence software. Two review team members will independently perform screening, full text review and data extraction. Results: Results will include a list of selection criteria and attributes of theoretical approaches for use in research on implementation strategy design. Descriptive data regarding selection criteria and attributes will be synthesized graphically and in table format. Data regarding the suggested use of theoretical approaches in implementation strategy design will be presented narratively. Conclusions: Results will be used to classify existing theoretical approaches according to the attributes and selection criteria identified in this scoping review. Envisioned next steps include an online tool that will be created to assist researchers in selecting theories, models, and frameworks for implementation strategy design.
Innovation management involvement among persons with disabilities in Malaysia
Authors: Yee Yen Yuen , Wu Jian Yong Eddie , Teoh Ming Yen Wendy 
Published: September 2022

Background: As Malaysia struggles with the battle to retain talented workers, the retention of persons with disabilities (PWDs) remains a major challenge in innovation management. Malaysia currently has the lowest retention of PWDs in innovation management in The Association of Southeast Asian Nations (ASEAN). The purpose of this study is to develop a unique framework to enhance the inclusion of PWDs in Malaysia. Methods: A questionnaire was distributed to 200 PWDs in the central region of Malaysia. Results: Based on the results of this research, four crucial variables (salary, compensation, career advancement and reward management) contributed to the lack of involvement in innovation management among PWDs in Malaysia. Conclusions: This study focuses on 200 PWDs in Malaysia. Despite the fact that PWDs’ involvement in innovation management is the lowest, there is a lack of research initiative and practitioner commitment to address this issue. Serving as preliminary research in Malaysia, this study develops a unique framework to fill the gap.