bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum steroid profiling of hepatocellular carcinoma associated with hyperadrenocorticism in dogs: A preliminary study
Authors: Oo Thandar , Sasaki Noboru , Ikenaka Yoshinori , Ichise Takahiro , Nagata Noriyuki , Yokoyama Nozomu , Sasaoka Kazuyoshi , Morishita Keitaro , Nakamura Kensuke , Takiguchi Mitsuyoshi 
Published: September 2022

BackgroundHepatocellular carcinoma (HCC) is one of the most common primary liver tumors in humans and dogs. Excessive adrenocortical hormone exposure may cause steroid hepatopathy, which may develop into HCC. In our previous study, hyperadrenocorticism (HAC) was a highly concurrent disease in dogs with HCC. Therefore, this study hypothesized that adrenal steroid alterations might be involved in hepatocarcinogenesis and aimed to specify the relationship between HAC and HCC in dogs.Materials and methodsThis study included 46 dogs brought to the Hokkaido University Veterinary Teaching Hospital between March 2019 and December 2020. Owners gave their signed consent for blood collection on their first visit. A total of 19 steroids (14 steroids and 5 metabolites) in the baseline serum of 15 dogs with HCC, 15 dogs with HAC, and 10 dogs with both diseases were quantitatively measured using the developed liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method.ResultsIn each group, 11 steroids were detected higher than 50%. The detection rate of steroid hormones did not significantly differ between the groups (p &gt; 0.05). Principle component analysis (PCA) showed that the steroid profiles of the three groups were comparable. Median steroid hormone concentrations were not significantly different between the study diseases (p &gt; 0.05).ConclusionThe developed LC/MS/MS was useful for measuring steroid hormones. Although it was clear that HAC was concurrent in dogs with HCC, none of the serum steroids was suggested to be involved in HCC.
Arbuscular Mycorrhizal Fungi Alleviate Salt Stress Damage by Coordinating Nitrogen Utilization in Leaves of Different Species
Authors: Ma Shilin , Yue Jianmin , Wang Jinping , Jia Zhaohui , Li Chong , Zeng Jingyi , Liu Xin , Zhang Jinchi 
Published: September 2022

With the intensification of coastal erosion, damage to coastal shelterbelts has gradually increased. Arbuscular mycorrhizal fungi (AMF) can improve the salinity tolerance and productivity of plants in saline–alkali soils using various strategies including nutrient uptake, osmotic regulation, soil shaping, etc. Thus, the application of AMF to alleviate the impacts of salinization for these shelterbelts has become a research hotspot. For this study, we investigated the effects of inoculation with different AMF strains on the growth and nitrogen (N) utilization of Gleditsia sinensis Lam. and Zelkova serrata (Thunb.) Makino leaves under different salt concentrations. As the salt concentration increased, the growth rates and leaf areas of the autoclaved AMF inoculant (CK) treatment exhibited a decreasing trend for both G. sinensis and Z. serrata, while Funneliformis mosseae (FM) and Corymbiglomus tortuosum (CT) treatments weakened this trend. Between them, on average, FM increased the G. sinensis height growth rate by 396.9%, ground diameter growth rate by 99.0%, and Z. serrata leaf area by 29.1%. At a salt concentration of 150 mM, the chlorophyll content and nitrate reductase activities of leaves under the FM treatment for both tree species were significantly higher than for CK, with an average increase in chlorophyll content of 106.1% and nitrate reductase activities by 74.6%. Moreover, the AMF inoculation significantly reduced the leaf N content and photosynthetic N-use efficiency of G. sinensis in contrast to Z. serrata. Further, in contrast to G. sinensis, the photosynthetic N-use efficiency was significantly positively correlated with the growth rate and leaf area of Z. serrata. Meanwhile, the nitrate reductase activity contributed most to the growth rate and leaf area of Z. serrata. Our results suggest that the issues with coastal shelterbelts might be effectively alleviated through appropriate AMF–plant combinations, which is of great significance for the optimization of forestry production.
Clustering Characterization of Acoustic Emission Signals Belonging to Twinning and Dislocation Slip during Plastic Deformation of Polycrystalline Sn
Authors: Tóth László Z. , Daróczi Lajos , Elrasasi Tarek Y. , Beke Dezső L. 
Published: September 2022

Results of acoustic emission (AE) measurements, carried out during plastic deformation of polycrystalline Sn samples, are analyzed by the adaptive sequential k-means method. The acoustic avalanches, originating from different sources, are separated on the basis of their spectral properties, that is, sorted into clusters, presented both on the so-called feature space (energy-median frequency plot) and on the power spectral density (PSD) curves. We found that one cluster in every measurement belongs to background vibrations, while the remaining ones are clearly attributed to twinning as well as dislocation slips at −30 °C and 25 °C, respectively. Interestingly, fingerprints of the well-known “ringing” of AE signals are present in different weights on the PSD curves. The energy and size distributions of the avalanches, corresponding to twinning and dislocation slips, show a bit different power-law exponents from those obtained earlier by fitting all AE signals without cluster separation. The maximum-likelihood estimation of the avalanche energy (ε) and size (τ) exponents provide ε=1.57±0.05 (at −30 °C) and ε=1.35±0.1 (at 25 °C), as well as τ=1.92±0.05 (at −30 °C) and τ= 1.55±0.1 (at 25 °C). The clustering analysis provides not only a manner to eliminate the background noise, but the characteristic avalanche shapes are also different for the two mechanisms, as it is visible on the PSD curves. Thus, we have illustrated that this clustering analysis is very useful in discriminating between different AE sources and can provide more realistic estimates, for example, for the characteristic exponents as compared to the classical hit-based approach where the exponents reflect an average value, containing hits from the low-frequency mechanical vibrations of the test machine, too.
Designing mRNA- and Peptide-Based Vaccine Construct against Emerging Multidrug-Resistant Citrobacter freundii: A Computational-Based Subtractive Proteomics Approach
Authors: Naveed Muhammad , Hassan Jawad-ul , Ahmad Muneeb , Naeem Nida , Mughal Muhammad Saad , Rabaan Ali A. , Aljeldah Mohammed , Shammari Basim R. Al , Alissa Mohammed , Sabour Amal A. , Alaeq Rana A. , Alshiekheid Maha A. , Turkistani Safaa A. , Elmi Abdirahman Hussein , Ahmed Naveed 
Published: September 2022

Background and Objectives: Citrobacter freundii (C. freundii) is an emerging and opportunistic Gram-negative bacteria of the human gastrointestinal tract associated with nosocomial and severe respiratory tract infections. It has also been associated with pneumonia, bloodstream, and urinary tract infections. Intrinsic and adaptive virulence characteristics of C. freundii have become a significant source of diarrheal infections and food poisoning among immune-compromised patients and newborns. Impulsive usage of antibiotics and these adaptive virulence characteristics has modulated the C. freundii into multidrug-resistant (MDR) bacteria. Conventional approaches are futile against MDR C. freundii. Materials and Methods: The current study exploits the modern computational-based vaccine design approach to treat infections related to MDR C. freundii. A whole proteome of C. freundii (strain: CWH001) was retrieved to screen pathogenic and nonhomologous proteins. Six proteins were shortlisted for the selection of putative epitopes for vaccine construct. Highly antigenic, nonallergen, and nontoxic eleven B-cell, HTL, and TCL epitopes were selected for mRNA- and peptide-based multi-epitope vaccine construct. Secondary and tertiary structures of the multi-epitope vaccine (MEVC) were designed, refined, and validated. Results: Evaluation of population coverage of MHC-I and MHC-II alleles were 72% and 90%, respectively. Docking MEVC with TLR-3 receptor with the binding affinity of 21.46 (kcal/mol) occurred through the mmGBSA process. Further validations include codon optimization with an enhanced CAI value of 0.95 and GC content of about 51%. Immune stimulation and molecular dynamic simulation ensure the antibody production upon antigen interaction with the host and stability of the MEVC construct, respectively. Conclusions: These interpretations propose a new strategy to combat MDR C. freundii. Further, in vivo and in vitro trials of this vaccine will be valuable in combating MDR pathogens.
Unraveling the Novel Bacterial Assisted Biodegradation Pathway of Morpholine
Authors: Kumar Rupak , Kapur Suman , Rao Vulichi Srinivasa 
Published: September 2022

Most xenobiotics are biodegradable, persistent or recalcitrant in nature. Morpholine, a typical xenobiotic, was initially regarded as recalcitrant, however, later proved to be biodegradable by bacterial species like Mycobacterium and Pseudomonas in particular. However, establishing the metabolic pathways involved for the successful biodegradation of morpholine is challenging because of its extreme level of water solubility that affects various analytical procedures. In addition, to date, no suitable analytical methods have been reported to directly estimate the activity of morpholine and its degradable products or intermediates. Nevertheless, methods, especially optical density, gas chromatography and mass spectrophotometric analysis, could indirectly estimate the degradation product(s) of morpholine formed as a result of its biotransformation. In this present study, the degradation pathway of morpholine was scertained by selected bacterial isolates by measuring their capacity to degrade morpholine. Based on this analysis of culture filtrates, it was determined that the novel isolate is the genus Halobacillus blutaparonensis which utilizes the diglycolic acid route from the metabolic degradation pathway of morpholine to induce one of two branches of the morpholine biodegradation pathway in the presence of morpholine while the other branch is inhibited. Whatever the branches with regard to the degradation pathway of morpholine exhibited by bacteria are, ammonia is the final end product of degradation which might be biochemically utilized by the isolate.
What enables elite athletes to maintain vigour during a pandemic? The importance of personal resources in coping with stress
Authors: Samełko Aleksandra , Szczypińska Marta , Guszkowska Monika 
Published: September 2022

The purpose of the study was to establish the relationship between the level of vigour during the first wave of the COVID -19 pandemic and selected personal resources for coping with stress : sense of coherence, hope for sucess and coping strategies. Participants of research were 57 individual sports players have been preparing for the Olympic Games in Tokyo, including 29 women and 28 men (aged 18 to 39 (M = 26.61, SD = 5.562). Methods: in the study conducted in April 2020, the following questionnaires were used: Profile of Mood State (POMS), The Hope for Success Questionnaire (HSQ), The Life Orientation Questionnaire (SOC- 29), The Inventory for Measuring Coping with Stress (Mini COPE).
Results: Significant relationshipp between vigour and two components of the sense of coherence were established: the sense of meaningfullness (r=0.566; p<0.001) and the sense of manageability (r=0.478; p<0,001), both dimensions of hope for success: willpower (r=0.485; p<0.001) and the ability to find sulutions (r=0.49; p = 0.001), helplessness strategy (r = -0.456; p <0.001).)
Conclusions: Strong relationships between vigour and the components of the sense of coherence confirm its important role in coping with stress by athletes during a pandemic, especially in maintaining a positive mood. Maintaining vigour by the athletes of the elite is fostered by hope for success, understood as a personality disposition. Active stress management strategies can be an effective way to maintain positive effect during the stressful period of a pandemic. Helplessness, in turn, can lower its level.
Catholic Church and a ministry of spiritual assistance in the dimension of sexuality development
Authors: Sorkowicz Anastazja 
Published: September 2022

In view of the omnipresent contemporary crisis of truth, it seems a purposeful and valuable activity to approximate, clarify, as well as correct or recify the vision of the Catholic Church's teaching regarding treatment of man as a sexual being. The purpose of the article is to attempt to present selected elements of the teachings of the Catholic Church on sexuality, considering the ways in which sexual education is understood and identifying persons who are most acccountable for that dimesion of education. The inquiry is conducted from the point of view of an educator. The source literature of this study consists of selected documents of the Catholic Church. Other studies on the topic were also analysed. Based on the analysis of the documents, it may be conculded, that the Catholic Church attaches great importance to the issues connected with sexuality and sexual education. Such education should be based on integral vision of man. It is emphasized that this sphere should be considered in the context of the development of the whole human being, whose fundamental taks in life is love understood as the gift of self. Sexual education is therefore tantamount to education for love. It involves assisting children and youth in their search for the answer to the most important questions concerning the meaning and purpose of life, as well as the ideas and values which determine the trajectory of human existence on Earth. An element of the analysed dimension of upbringing is both the introduction of young people to issues related in a strict sense to the biological functioning of man as a being endowed with sex, and the formation of views and attitudes towards himself and other people. Given the strength and primordiality of the natural family bonds, this responsible task should be delegated mainly to parents. It is parents who are to the greatest degree responsible for the formation of children to whom they have passed on their lives. All other educational instituations should conform to the principle of subsidiarity and respect the precedence of parental rights and obligations. The ministry of spiritual assistance in the delicate sphere of the development of sexuality is the task of parents, who are the most important educators. All parents are obliged to undertake that difficult task, which requires creation of the climate of trust, closeness and mutual respect in the family, as well as gentleness and respect for the intimate shpere of children. It is essential, that all information be conveyd in a clear way and reach children in proper time.
Attitudes of spouses towards abortion and the quality of their mutual relations
Authors: Rawicka Iwona 
Published: September 2022

In recent years there have been a lot of social and political discussions concerning legal aspects of abortion in Poland and lessening restrictions in this area. Nevertheless there are few debates on social, psychological and medical effects for women and men who take the decision of abortion both on individual basis and in their mutual relationship. In the article the research has been conducted concerning relation between women’s and men’s attitudes towards abortion and the quality of their marital relationship. It cannot be explicitly stated what results abortion can bring in their common relationship. However, it can be estimated to what extend the cognitive, emotional and motivational aspect of their attitude towards abortion is connected with the quality of their marital bonding, love, communication and the feeling of being well-matched. In the research the method of diagnostic survey was used. To collect data estimating the quality of marriage the following four standardized exploratory tools were applied: Marital Bonding Scale of Józef Szopiński, Love Measure Questionnaire according to Robert Sternberg, adopted by Bogdana Wojciszke, Marital Communication Questionnaire by Maria Kaźmierczak and Mieczysław Plopa, and Marital Match Questionnaire by Jan Rostowski and Mieczysław Plopa. In order to estimate the respondents’ attitude towards abortion the researchers used one of the statements from the scale of attitude towards parenthood by Marta Komorowska-Pudło. On the basis of the data collected in the research it can be diagnosed that the attitudes of a married couple towards abortion have a statistically significant association with their mutual relationship, however not a big one. The higher level of acceptance they expressed for the abortion in the situation of an unplanned pregnancy ,the lower was the level of the satisfaction in their marital bonding, love, communication and feeling of being well-matched. At the same time with the increase of their level of satisfaction in their mutual relationship, their negative attitude towards abortion as a birth control tool was also growing. On the basis of comparison of the research results with data presented in the literature regarding the quality of married couples who had abortion, one can conclude that the attitude of married couples towards abortion in reference to cognitive and emotional-motivational components are connected in a similar way with the quality of their relationship, as in case of the attitude of married couple towards abortion in behavioral component aspect. Accepting abortion (pro-choice attitude) is connected with less satisfactory relationships between men and women in marriages. On the other hand the lack of acceptance of abortion (pro-life attitude) results in the higher level of the quality of their relationship.
Analysis of the menstrual chart observation characteristics, meeting the typical cycle criteria among women of procreation age according to the symptom-thermal double-check method
Authors: Piasecka Katarzyna 
Published: September 2022

Background: Knowledge concerning value of menstrual cycle observation and its use to monitor procreative health is underestimated and limited in women's health promotion, education and health care. Academy of Pediatrics, Committee on Adolescence and , American College of Obstetricians and Gynecologists recommends starting to observe the forming cycle as early as puberty in order to pre-screen girls , from groups at risk of fertility disorders such as polycystic ovary syndrome. In view of the numerous reports on the increase in fertility problems, such a simple tool as self-observation of the menstrual cycle becomes particularly useful to learn about the natural rhythm of fertility and in the case of abnormalities, i.e. deviation from the typical course shortens the time to the first medical consultation. In Poland there are several non-governmental non-profit organizations, which through certified teachers of fertility awareness methods can professionally support the teaching of adolescents, adults and interested people working in the health service in the field of fertility awareness, health education and natural family planning.
Aim of the presented study was to analyze 105 menstrual cycle observation charts among polish women not using contraception according to the principles of the symptom-thermal double-check method.
Method: The study was performed using the method of documentation analysis, 105 menstrual cycle observation charts using the statistical package SPSS Statistica version 25. The level of p < 0.05 was considered statistically significant.
Results: The average age of the women studied was 29 years. The majority of the women , i.e. more than 58%, were unmarried and childless (79.1%). The average length of the menstrual cycle was: 28.6 days. The average duration of the luteal phase was almost 13 days. The average length of days of highly fertile mucus was nearly 4 days. The average length of cycles of women over 35 years of age was: 28.88 days, while for women under 35 years of age. 28.48 days. The age of onset of the first menstrual period of the women studied varied between 10 and 17 years of age.
Conclusions: The studied group of women was homogeneous in terms of the adopted eligibility criteria, i.e. they had typical cycles and did not use contraceptive methods. The examined parameters of the menstrual cycle, i.e.: the average length of the cycle , the course of the luteal phase and the average length of the occurrence of highly fertile mucus met the criteria for a normal cycle according to the symptom-thermal double-check method.
Cardiovascular disease risk factors in the Lubelskie Voivodeship in 2008-2018 - part I: tobacco smoking
Authors: Jankowski Krzysztof 
Published: September 2022

Background: Smoking is one of the most common cardiovascular risk factors. It is responsible for 7 million deaths annually in the world. Another 1.2 million people die from passive smoking. Nicotinism causes a chronic inflammatory process, oxidative stress, and it also works by increasing adrenergic arousal. The Cardiovascular Diseases Prevention Program implemented by Primary Healthcare Clinics is designed to assess the risk factors for cardiovascular events in patients. This provides an opportunity for an early response and appropriate action.
Methods: A retrospective analysis of data obtained by the National Health Fund as part of the implementation of 86,485 preventive cards of the Cardiovascular Diseases Prevention Program in the Lubelskie Voivodeship in 2008-2018 was carried out. The analyzes were performed using the IBM SPSS Statistics for Windows, version 25 program. The chi-square test and the Pearson linear correlation between the quantitative features were used to test the relationship between the two qualitative features. Significant predictors of a risk factor for cardiovascular disease were determined using multivariate logistic regression. The significance level was assumed to be α = 0.05 for two-tailed tests.
Results: Tobacco smoking was declared by 26.5% of all respondents. It was significantly more often declared by men. The percentage of smokers increased with age - the highest was obtained for the group of 55-year-olds. Smoking cigarettes was significantly more often reported by people with primary education (44%), manual workers (33%) and retirees / pensioners (35%). Smoking tobacco escalated the risk of being diagnosed with arterial hypertension by 16.5%. It significantly influenced the risk of diabetes - even by 33.9%. It raised the risk of hypercholesterolaemia by 17.1% compared to never-smokers as well.
Conclusions: The prevalence of nicotinism is alarmingly high. The effective implementation of the Cardiovascular Diseases Prevention Program provides an opportunity to educate on a large scale and identify patients at high risk of a cardiovascular event. Assessing the risk of death on the SCORE scale can motivate patients to change their lifestyle and make them aware of the impact of smoking on increasing this risk.