bluuebunny/crossref_metadata_2025_split huggingface dataset:
Post-Disaster Reconstruction, Well-being and Sustainable Development Goals: A Conceptual Framework
Authors: Tiwari Piyush , Shukla Jyoti 
Published: October 2022

Climate change has increased the frequency and intensity of disasters. Low-income households living in low- and middle-income countries are most vulnerable to the effects of disasters. While efforts have increased for better disaster management since the adoption of the Sendai framework adopted at the Third UN World Conference on Disaster Risk Reduction in Japan in 2015 by 187 countries, the success rate of post-disaster reconstruction and rehabilitation has been debatable for disaster affected persons. The purpose of this article (which is the first part of our research on approach to post disaster reconstruction) is to propose a framework based on capability approach to understand the determinants of well-being that need to be restored for disaster affected persons. The second part will present results from application of this framework to examine the post-disaster reconstruction of affected persons in Chennai (India). Resettlement is a complex issue, and it requires a balance between protecting lives and livelihoods of those affected, as these are tied to a location, and protecting them from future disasters, the occurrence of which is also tied to a location. The article argues that a capabilities-based approach that aligns with sustainable development goals could provide a framework to identify components of a post-disaster management plan to satisfactorily rebuild the well-being of affected persons.
The impact of the COVID-19 pandemic on the mental health and wellbeing of refugees and asylum seekers-A Narrative Review of the Literature
Authors: El Tatary Ghassan , Gill Neeraj 
Published: October 2022

Objective To identify the factors affecting refugees and asylum seekers mental health and wellbeing during the COVID-19 pandemic. Methods A narrative review of available international research literature from January 2020 to June 2021 was conducted to identify these factors. Results Seven factors were identified: pre-existing physical health vulnerabilities, pre-existing mental health vulnerabilities, environmental, social, cultural-specific, economic and legal/welfare challenges. Conclusion Multiple interacting bio-psycho-socio-economic-cultural-ecological factors lead to greater impact of COVID-19 pandemic on refugee and asylum seekers wellbeing than the general population.
Design-Related Impacts on End-of-Life Experience: A Brief Report of Findings from an Exploratory Qualitative Study
Authors: Knox Michelle 
Published: October 2022

Background Despite the general preference to die at home, many deaths occur in institutionalized settings. While biomedical interventions to ameliorate end-of-life (EoL) suffering have advanced, the end-of-life care (EoLC) environment is less understood as a means of palliative support. Objective This exploratory study considered the implications of clinical EoLC environments (facility buildings and their adjacent areas), aiming to understand how these designed spaces may be improved to better support experiences for patients, families, and staff. Methods Using an ethnography-driven approach, field observations (including participant commentaries) were captured at a standalone hospice and a palliative care ward at a general hospital. These were supplemented with semi-structured interviews. Content and thematic analyses were performed based on an interpretive-descriptive paradigm. Finally, informed by a review of field literature, analyses of all data were inter-related, and an interpretation was built to highlight key design considerations. Results Through the analysis, 6 socio-spatial dimensions of EoLC facilities were identified (locational contexts; service administration & management; common spaces; private spaces; in-between spaces; and nature-integrated spaces) as guiding concepts to appraise and improve such settings. Conclusion Physical, emotional, and social wellbeing at the end of life is coalesced in and made visible by the designed environment. Therefore, evidence-based design serves as an important non-clinical intervention in such settings; however, patient involvement in such research remains difficult. Future scholarly research, new building schemes, and renovation projects should further examine the socio-spatial functions of clinical EoLC environments and investigate the challenges surrounding patient engagement within this domain.
Impact of the COVID-19 Controlled Drugs and Substances Act exemption on pharmacist prescribing of opioids, benzodiazepines and stimulants in Ontario: A cross-sectional time-series analysis
Authors: Chang Ann , Chaudhry Shanzeh , McCormack Daniel , Gomes Tara , Shivji Anisa , Tadrous Mina 
Published: October 2022

Background: Due to the coronavirus disease 2019 (COVID-19) pandemic, Health Canada issued an exemption to the Controlled Drugs and Substances Act (CDSA) on March 19, 2020, enabling pharmacists to act as prescribers of controlled substances to support continuity of care. Our study investigates utilization of the CDSA exemption by Ontario pharmacists with the intent to inform policy on pharmacist scope of practice and to improve future patient outcomes. Methods: We conducted a time-series analysis of pharmacist-prescribed opioid, benzodiazepine and stimulant claims data using Ontario Narcotics Monitoring System (NMS) data between January 2019 and December 2021. We used ARIMA modelling to measure the change to these classes of claims and to opioid claims containing quantities greater than a 30-day supply. Results: Postexemption, the average weekly number of pharmacist-prescribed opioid, benzodiazepine and stimulant claims rose by 146% (160 to 393 claims/week), 960% (49 to 515 claims/week) and 2150% (8 to 177 claims/week), respectively. There was a 2-week lag period between the time of announcement and the statistically significant increase in claims on April 5, 2020( p < 0.0001). The total number of claims for opioid quantities exceeding a 30-day supply decreased by 60%. Cumulative pharmacist-prescribed claims accounted for under 2% of the total NMS claims. Interpretation: Ontario pharmacists used the CDSA exemption but were prescribing at low rates. These findings suggest an effective change to pharmacy practice as the low rates show pharmacists used the exemption as a last line of defense. This may lead to further studies exploring treatment breaks during the COVID-19 pandemic and future changes to pharmacist scope to benefit patients.
A Proposal for a Standardized Imaging Algorithm to Improve the Accuracy and Reliability for the Diagnosis of Thoracolumbar Posterior Ligamentous Complex Injury in Computed Tomography and Magnetic Resonance Imaging
Authors: Aly Mohamed M. , Al-Shoaibi Abdulbaset M. , Aljuzair Ali H. , Issa Tariq Ziad , Vaccaro Alexander R. 
Published: October 2022

Study Design Systematic Literature Review. Objective To propose a systematic imaging algorithm for diagnosing posterior ligamentous complex (PLC) injury in computed tomography (CT) and magnetic resonance imaging (MRI) to improve the reliability of PLC assessment. Methods A systematic review was conducted following PRISMA guidelines. The Scopus database was searched from its inception until July 21, 2022, for studies evaluating CT or MRI assessment of the PLC injury following thoracolumbar trauma. The studies extracted key findings, objectives, injury definitions, and radiographic modalities. Results Twenty-three studies were included in this systematic review, encompassing 2021 patients. Five studies evaluated the accuracy of MRI in detecting thoracolumbar PLC injury using intraoperative findings as a reference. These studies indicate that black stripe discontinuity due to supraspinous or ligamentum flavum rupture is a more specific criterion of PLC injury than high-signal intensity. Thirteen papers evaluated the accuracy or reliability of CT in detecting thoracolumbar PLC injury using MRI or intraoperative findings as a reference. The overall accuracy rate of CT in detecting PLC injury was 68-90%. Two studies evaluate the accuracy of combined CT findings, showing that ≥2 CT findings are associated with a positive predictive value of 88-91 %. Vertebral translation, facet joint malalignment, spinous process fracture, horizontal laminar fracture, and interspinous widening were independent predictors of PLC injury. Conclusion We provided a comprehensive imaging algorithm for diagnosing PLC in CT and MRI based on available literature and our experience. The algorithm will potentially improve the accuracy and reliability of PLC assessment, however it needs multicentre prospective validation.
Critical Care Nurses’ Experiences During the Illness of Family Members: A Qualitative Study
Authors: Khatib Wadad El , Fawaz Mirna , Al-Shloul Mohammad N. , Rayan Ahmad , ALBashtawy Mohammed , Al-Amer Rasmieh M. , Abdalrahim Asem , Khatatbeh Moawiah 
Published: October 2022

Introduction A loved one's hospitalization in a critical care unit is a traumatic experience for families. However, because of their status and professional competence, a family member who is also a critical care nurse has additional obstacles and often long-term consequences. Objectives To describe the experiences of critical care nurse-family members when a loved one is admitted to a critical care unit at the Hotel-Dieu de France hospital. Methods A qualitative path based on van Manen's hermeneutic phenomenology combining both descriptive and interpretive models were adopted. Results The lived experience of critical care nurses in providing care for their family members admitted into the same critical care were summarized in five themes. Nurses were torn between roles, consisting of confounding roles, their registered nurse status, and watchfulness. The lived experience of critical care nurses in providing care for their family members admitted into the same critical care was summarized into specialized knowledge that included a double-edged sword of seeking information and difficulty delivering the information. Critical nurses compete for expectations, including those placed on self and family members, resulting in emotional and personal sacrifice while gaining insight into the experiences. Conclusions Critical care nurse-family members have a unique experience compared to the rest of the family, necessitating specialized care and attention. Increased awareness among healthcare providers could be a start in the right direction.
Implementation of advance care planning decision aids for patients undergoing high-risk surgery: a field-testing study
Authors: Yamamoto Kanako , Kaido Toshimi , Yokoi Tadao , Shimada Gen , Taketa Takashi , Nakayama Kazuhiro 
Published: October 2022

Abstract
Background
Patients undergoing high-risk surgery are at a risk of sudden deterioration of their health. This study aimed to examine the feasibility of the development of two patient decision aids (PtDAs) to assist patients undergoing high-risk surgeries in informed decision-making about their medical care in a crisis.

Methods
This field testing implemented two PtDAs that met the international criteria developed by the researchers for patients before surgery. Study participants were patients scheduled to be admitted to the intensive care unit after surgery at one acute care hospital in Japan and their families. The study used a mixed-methods approach. The primary outcome was patients’ decision satisfaction evaluated by the SURE test. Secondary outcomes were the perception of the need to discuss advance care planning (ACP) before surgery and mental health status. The families were also surveyed on their confidence in proxy decision-making (NRS: 0–10, quantitative data). In addition, interviews were conducted after discharge to assess the acceptability of PtDAs. Data were collected before (preoperative outpatients, baseline: T0) and after providing PtDAs (in the hospital: T1) and following discharge (T2, T3).

Results
Nine patients were enrolled, of whom seven agreed to participate (including their families). The SURE test scores (mean ± SD) were 2.1 ± 1.2 (T0), 3.4 ± 0.8 (T2), and 3.9 ± 0.4 (T3). The need to discuss ACP before surgery was 8.7 ± 1.3 (T1) and 9.1 ± 0.9 (T2). The degree of confidence in family surrogate decision-making was 6.1 ± 2.5 (T0), 7.7 ± 1.4 (T1), and 8.1 ± 1.5 (T2). The patients reported that using PtDAs provided an opportunity to share their thoughts with their families and inspired them to start mapping their life plans. Additionally, patients wanted to share and discuss their decision-making process with medical professionals after the surgery.

Conclusions
PtDAs supporting ACP in patients undergoing high-risk surgery were developed, evaluated, and accepted. However, they did not involve any discussion of patients’ ACP treatment wishes with their families. Medical providers should be coached to provide adequate support to patients. In the future, larger studies evaluating the effectiveness of PtDAs are necessary.
Impact of interactive multi-media learning for physicians in musculoskeletal education – a pilot study
Authors: Wadey Veronica , Okoro Tosan , Sathiyamoorthy Thrmiga , Snowdon David , McDonald-Blumer Heather , Cividino Alfred , Kopansky-Giles Deborah , Levy David , Freeman Risa , Herold Jodi , Archibald Douglas 
Published: October 2022

Abstract
Background
The aim of this educational study was to investigate the use of interactive case-based modules relating to the screening and identification of early-stage inflammatory arthritis in both online technology (OLT) and paper (PF) formats with identical content.

Methods
Forty learners from family medicine or rheumatology residency programs were recruited. Content pertaining to a “Sore Hands, Sore Feet” (SHSF) and Gait Arms Legs Spine (GALS) screening tool modules were selected, reviewed and developed based on a validated curriculum from the World Health Organization and Canadian Curriculum for MSK conditions. Both the SHSF module and GALS screening tool were assessed via a randomized control trial. Assessments were completed during an orientation with all learners; then prior to the intervention (T1); at the end of the module (T2) and 3 months following the modules (T3) to assess retention. Focus groups were conducted to determine learners’ satisfaction with the different learning formats. Baseline data was collated, and analysis performed after randomization into the PF (control) and OLT (experimental) groups. Repeated measures ANOVA was used for statistical analyses.

Results
Forty participants were recruited and randomized into the PF or OLT group (n = 20 each). At 3 months, there were n = 31 participants for SHSF (PF n = 19, OLT n = 12) and n = 32 for GALS (PF n = 19, OLT n = 13). There was no significant difference between the OLT and PF groups in both analyses. A significant increase in scores from Pre- to Post-Module in SHSF (F (1, 18) = 24.62. p < .0001) and GALS (F (1, 30) = 40.08, p < .0001) were identified to suggest learning occurred with both formats. The repeated measures ANOVA to assess retention revealed a significant decrease in scores from Post-Module to Follow-up for both learning format groups for SHSF (F (1, 29) = 4.68. p = .039), and GALS (F (1, 30) = 18.27. p < .0001) suggesting 3 months may be too long to retain this educational information.

Conclusions
Both formats led to residents’ ability to screen, identify and initially manage inflammatory arthritis. The hypothesis is rejected because both OLT and PF groups demonstrated significant learning during the process regardless of format. It is important to emphasize that from T1 (pre-module) to T2 (post-module), the residents demonstrated learning regardless of group to which they were assigned. However, learning retention declined from T2 (post-module) to T3 (three-month follow-up). Regular review of knowledge may be required earlier than 3 months to retain information learned. This study may impact educational strategies in MSK health.

Trial registration
This study did not involve “patients” rather learners and as such it was not registered.
Ultra-sensitive molecular detection of gene fusions from RNA using ASPYRE
Authors: Gray Eleanor R , Mordaka Justyna M , Christoforou Efthimia R , von Bargen Kristine , Potts Nicola D , Xyrafaki Christina , Silva Ana-Luisa , Stolarek-Januszkiewicz Magdalena , Anton Katarzyna , Powalowska Paulina K , Andreazza Simonetta , Tomassini Alessandro , Palmer Rebecca N , Cooke Aishling , Osborne Robert J , Balmforth Barnaby W 
Published: October 2022

Abstract
Background
RNA is a critical analyte for unambiguous detection of actionable mutations used to guide treatment decisions in oncology. Currently available methods for gene fusion detection include molecular or antibody-based assays, which suffer from either being limited to single-gene targeting, lack of sensitivity, or long turnaround time. The sensitivity and predictive value of next generation sequencing DNA-based assays to detect fusions by sequencing intronic regions is variable, due to the extensive size of introns. The required depth of sequencing and input nucleic acid required can be prohibitive; in addition it is not certain that predicted gene fusions are actually expressed.

Results
Herein we describe a method based on pyrophosphorolysis to include detection of gene fusions from RNA, with identical assay steps and conditions to detect somatic mutations in DNA [1], permitting concurrent assessment of DNA and RNA in a single instrument run.

Conclusion
The limit of detection was under 6 molecules/ 6 µL target volume. The workflow and instrumentation required are akin to PCR assays, and the entire assay from extracted nucleic acid to sample analysis can be completed within a single day.
Molecular characteristics of a coxsackievirus A12 strain in Zhejiang of China, 2019
Authors: Hu Linjie , Zhou Lu , Wang Pingping , Maimaiti Hairenguli , Lu Yihan 
Published: October 2022

Abstract
Background
Enterovirus A (EV-A), such as enterovirus A71 (EV-A71), generally causes hand, foot, and mouth disease (HFMD). However, limited studies focused on uncommon enterovirus serotypes such as coxsackievirus A12 (CV-A12). This study aimed to provide evidence to determine the molecular characteristics of a CV-A12 strain isolated in Zhejiang province, China.

Methods
In routine surveillance of HFMD, we identified a child case with CV-A12 infection in 2019 in Zhejiang province, China. Enterovirus was examined by using real-time reverse transcription-PCR (qRT-PCR). A partial VP1 sequence was amplified to determine the serotype, and then a full-length CV-A12 genome was sequenced. Nucleotide and amino acid similarity was calculated with those CV-A12 strains available in GenBank. Recombination was detected using RDP 4 and SimPlot. Furthermore, phylogenetic analysis was conducted by using BEAST 1.10, and protein modeling was performed with I-TASSER webserver.

Results
A full-length CV-A12 genome PJ201984 was isolated in a Chinese child with HFMD. The similarities with complete coding sequences of the CV-A12 strains in GenBank ranged between 79.3–100% (nucleotide) and 94.4–100% (amino acid), whereas it was 88.7–100.0% (nucleotide) and 97.2–100% (amino acid) when excluding the CV-A12 prototype strain Texas-12. In PJ201984, amino acid variations were more divergent in P2 and P3 regions than those in P1; the majority of those variations in VP1 (13/15) and VP4 (7/8) were similar to those documented in recently isolated CV-A12 strains in China. Furthermore, recombination was identified in P2 region, which involved a CV-A5 strain collected in China. Phylogenetic analysis revealed that PJ201984 clustered together with multiple CV-A12 strains isolated in China and the Netherlands during 2013–2018, as compared to another cluster consisting of CV-A12 strains in China and France during 2009–2015. Additionally, protein models of VP1 and VP4 in PJ201984 were well predicted to be similar to VP1 protein of EV-A71 and VP4 protein of coxsackievirus A21, respectively.

Conclusions
The full-length CV-A12 genome was characterized to have common recombination in P2 region and be phylogenetically related to those CV-A12 strains isolated in recent years, suggesting a continual spread in China. It warrants strengthening the routine surveillance for uncommon enterovirus serotypes, particularly on possible recombination and variation.
Accuracy of next-generation sequencing for molecular profiling of small specimen of lung cancer: a prospective pilot study of side-by-side comparison
Authors: Ben Xiaosong , Tian Dan , Zhuang Weitao , Chen Rixin , Wang Sichao , Zhou Zihao , Deng Cheng , Shi Ruiqing , Liu Songlin , Zhang Dongkun , Tang Jiming , Xie Liang , Zhou Haiyu , Zhang Zhou , Li Min , Zhang Xuanye , Qiao Guibin 
Published: October 2022

Abstract
Background
Successful practice of precision medicine in advanced lung cancers relies on therapeutic regimens tailored to individual molecular characteristics. The aim of this study was to investigate the accuracy of small specimens for molecular profiling using next-generation sequencing (NGS).

Methods
Genetic alternations, tumor mutational burden (TMB), status of microsatellite instability (MSI), and expression of programmed death ligand 1 (PD-L1) were compared side-by-side between the concurrently obtained core needle biopsy (CNB) and resection specimens in 17 patients with resectable non-small cell lung cancers.

Results
DNA yield and library complexity were significantly lower in CNB specimens (both p < 0.01), whereas the insert size, sequencing depth, and Q30 ratio were similar between the matched specimens (all p > 0.05). The total numbers of genetic alternations detected in resection and CNB specimens were 186 and 211, respectively, with 156 alternations in common, yielding a specific concordance rate of 83.9%. The prevalence of mutations in 8 major driver genes was 100% identical between surgical and CNB specimens, though the allele frequency was lower in CNB specimens, with a median underestimation of 57%. Results of TMB were similar (p = 0.547) and MSI status was 100% matched in all paired specimens.

Conclusions
Pulmonary CNB specimens were suitable for NGS given the satisfactory accuracy when compared to corresponding surgical specimens. NGS results yielding from CNB specimens should be deemed reliable to provide instructive information for the treatment of advanced lung cancers.