bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitigation of tobacco bacteria wilt with microbial degradation of phenolic allelochemicals
Authors: Chang Xiaohan , Wang Yi , Sun Jingguo , Xiang Haibo , Yang Yong , Chen Shouwen , Yu Jun , Yang Chunlei 
Published: December 2022

AbstractLong-term continuous monoculture cropping of tobacco leads to high incidence of tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum, which threatening world tobacco production and causing great economy loss. In this study, a safe and effective way to control TBW by microbial degradation of phenolic allelochemicals (PAs) was explored. Eleven kinds of PAs were identified from continuous tobacco cropping soil. These PAs exhibited various effects on the growth, chemotaxis and biofilm formation of R. solanacearum. Then we isolated eight strains of Bacillus, one strain of Brucella, one strain of Enterobacter and one strain of Stenotrophomonas capable of degrading these PAs. The results of degradation assay showed that these isolated strains could degrade PAs both in culture solutions and soil. Besides, the incidence of TBW caused by R. solanacearum and deteriorated by PAs were significantly decreased by treating with these degrading strains. Furthermore, six out of eleven isolated strains were combined to degrade all the identified PAs and ultimately sharply reduced the incidence of TBW by 61.44% in pot experiment. In addition, the combined degrading bacteria could promote the plant growth and defense response. This study will provide a promising strategy for TBW control in tobacco production.
Hybrid the long short-term memory with whale optimization algorithm and variational mode decomposition for monthly evapotranspiration estimation
Authors: Fu Tonglin , Li Xinrong 
Published: December 2022

AbstractThe sustainability of artificial sand-binding vegetation is determined by the water balance between evapotranspiration (ET) and precipitation in desert regions. Consequently, accurately estimating ET is a critical prerequisite for determing the types and spatial distribution of artificial vegetation in different sandy areas. For this purpose, a novel hybrid estimation model was proposed to estimate monthly ET by coupling the deep learning long short term memory (LSTM) with variational mode decomposition (VMD) and whale optimization algorithm (WOA) (i.e., VMD-WOA-LSTM) to estimate the monthly ET in the southeast margins of Tengger Desert. The superiority of LSTM was selected due to its capability of automatically extracting the nonlinear and nonstationary features from sequential data, WOA was employed to optimize the hyperparameters of LSTM, and VMD was used to extract the intrinsic traits of ET time series. The estimating results of VMD-WOA-LSTM has been compared with actual ET and estimation of other hybrid models in terms of standard performance metrics. The results reveale that VMD-WOA-LSTM provide more accurate and reliable estimating results than that of LSTM, the support vector machine (SVM), and the variants of those models. Therefore, VMD-WOA-LSTM could be recommended as an essential auxiliary method to estimate ET in desert regions.
Unsupervised real-world knowledge extraction via disentangled variational autoencoders for photon diagnostics
Authors: Hartmann Gregor , Goetzke Gesa , Düsterer Stefan , Feuer-Forson Peter , Lever Fabiano , Meier David , Möller Felix , Vera Ramirez Luis , Guehr Markus , Tiedtke Kai , Viefhaus Jens , Braune Markus 
Published: December 2022

AbstractWe present real-world data processing on measured electron time-of-flight data via neural networks. Specifically, the use of disentangled variational autoencoders on data from a diagnostic instrument for online wavelength monitoring at the free electron laser FLASH in Hamburg. Without a-priori knowledge the network is able to find representations of single-shot FEL spectra, which have a low signal-to-noise ratio. This reveals, in a directly human-interpretable way, crucial information about the photon properties. The central photon energy and the intensity as well as very detector-specific features are identified. The network is also capable of data cleaning, i.e. denoising, as well as the removal of artefacts. In the reconstruction, this allows for identification of signatures with very low intensity which are hardly recognisable in the raw data. In this particular case, the network enhances the quality of the diagnostic analysis at FLASH. However, this unsupervised method also has the potential to improve the analysis of other similar types of spectroscopy data.
Impact of high Fe-concentrations on microbial community structure and dissolved organics in hydrothermal plumes: an experimental study
Authors: Hansen Christian T. , Kleint Charlotte , Böhnke Stefanie , Klose Lukas , Adam-Beyer Nicole , Sass Katharina , Zitoun Rebecca , Sander Sylvia G. , Indenbirken Daniela , Dittmar Thorsten , Koschinsky Andrea , Perner Mirjam 
Published: December 2022

AbstractIron (Fe) is an essential trace element for life. In the ocean, Fe can be exceptionally scarce and thus biolimiting or extremely enriched causing microbial stress. The ability of hydrothermal plume microbes to counteract unfavorable Fe-concentrations up to 10 mM is investigated through experiments. While Campylobacterota (Sulfurimonas) are prominent in a diverse community at low to intermediate Fe-concentrations, the highest 10 mM Fe-level is phylogenetically less diverse and dominated by the SUP05 clade (Gammaproteobacteria), a species known to be genetically well equipped to strive in high-Fe environments. In all incubations, Fe-binding ligands were produced in excess of the corresponding Fe-concentration level, possibly facilitating biological Fe-uptake in low-Fe incubations and detoxification in high-Fe incubations. The diversity of Fe-containing formulae among dissolved organics (SPE-DOM) decreased with increasing Fe-concentration, which may reflect toxic conditions of the high-Fe treatments. A DOM-derived degradation index (IDEG) points to a degradation magnitude (microbial activity) that decreases with Fe and/or selective Fe-DOM coagulation. Our results show that some hydrothermal microbes (especially Gammaproteobacteria) have the capacity to thrive even at unfavorably high Fe-concentrations. These ligand-producing microbes could hence play a key role in keeping Fe in solution, particularly in environments, where Fe precipitation dominates and toxic conditions prevail.
Dialysis based-culture medium conditioning improved the generation of human induced pluripotent stem cell derived-liver organoid in a high cell density
Authors: Torizal Fuad Gandhi , Utami Tia , Lau Qiao You , Inamura Kousuke , Nishikawa Masaki , Sakai Yasuyuki 
Published: December 2022

AbstractHuman pluripotent stem cell-derived liver organoids (HLOs) have recently become a promising alternative for liver regenerative therapy. To realize this application, a large amount of human-induced pluripotent stem cells (hiPSCs) derived-liver cells are required for partial liver replacement during transplantation. This method requires stepwise induction using costly growth factors to direct the hiPSCs into the hepatic lineage. Therefore, we developed a simple dialysis-based medium conditioning that fully utilized growth factors accumulation to improve hepatic differentiation of hiPSCs at a high cell density. The results demonstrated that the dialysis culture system could accumulate the four essential growth factors required in each differentiation stage: activin A, bone morphogenetic protein 4 (BMP4), hepatocyte growth factor (HGF), and oncostatin M (OSM). As a result, this low lactate culture environment allowed high-density bipotential hepatic differentiation of up to 4.5 × 107 cells/mL of human liver organoids (HLOs), consisting of hiPSC derived-hepatocyte like cells (HLCs) and cholangiocyte like-cells (CLCs). The differentiated HLOs presented a better or comparable hepatic marker and hepatobiliary physiology to the one that differentiated in suspension culture with routine daily medium replacement at a lower cell density. This simple miniaturized dialysis culture system demonstrated the feasibility of cost-effective high-density hepatic differentiation with minimum growth factor usage.
Anatomical study for elucidating the stabilization mechanism in the trapeziometacarpal joint
Authors: Norose Mio , Nimura Akimoto , Tsutsumi Masahiro , Fujita Koji , Okawa Atsushi , Akita Keiichi 
Published: December 2022

AbstractTo determine the pathogenesis of trapeziometacarpal (TMC) joint instability, which leads to osteoarthritis, we investigated the anatomical relationships among the surrounding ligaments, muscles (first dorsal interosseous [FDI] and opponens pollicis [OPP]), and joint capsule. We examined the bone morphology and cortical bone thickening in 25 cadaveric thumbs using micro-computed tomography and performed macroscopic and histological analyses. The dorsal trapezium had a tubercle with cortical bone thickening, corresponding to the attachment of the FDI aponeurosis intermingled with the joint capsule. Radially, the thin joint capsule was observed to underlie the muscular part of the OPP. Therefore, the dorsal ligaments, which have been previously considered static stabilizers, could be interpreted as parts of the capsuloaponeurotic complex consisting of the FDI aponeurosis and joint capsule. In the radial aspect, muscular OPP activation may be essential for TMC joint stabilization. Our findings may contribute to the appropriate management of TMC osteoarthritis.
Arg/Lys-containing IDRs are cryptic binding domains for ATP and nucleic acids that interplay to modulate LLPS
Authors: Dang Mei , Li Tongyang , Zhou Shibo , Song Jianxing 
Published: December 2022

AbstractMost membrane-less organelles (MLOs) formed by LLPS contain both nucleic acids and IDR-rich proteins. Currently while IDRs are well-recognized to drive LLPS, nucleic acids are thought to exert non-specific electrostatic/salt effects. TDP-43 functions by binding RNA/ssDNA and its LLPS was characterized without nucleic acids to be driven mainly by PLD-oligomerization, which may further transit into aggregation characteristic of various neurodegenerative diseases. Here by NMR, we discovered unexpectedly for TDP-43 PLD: 1) ssDNAs drive and then dissolve LLPS by multivalently and specifically binding Arg/Lys. 2) LLPS is driven by nucleic-acid-binding coupled with PLD-oligomerization. 3) ATP and nucleic acids universally interplay in modulating LLPS by competing for binding Arg/Lys. However, the unique hydrophobic region within PLD renders LLPS to exaggerate into aggregation. The study not only unveils the first residue-resolution mechanism of the nucleic-acid-driven LLPS of TDP-43 PLD, but also decodes a general principle that not just TDP-43 PLD, all Arg/Lys-containing IDRs are cryptic nucleic-acid-binding domains that may phase separate upon binding nucleic acids. Strikingly, ATP shares a common mechanism with nucleic acids in binding IDRs, thus emerging as a universal mediator for interactions between IDRs and nucleic acids, which may underlie previously-unrecognized roles of ATP at mM in physiology and pathology.
Molecularly “clicking” active moieties to germanium-based inorganic 2D materials
Authors: Palacios-Corella Mario , Muñoz Jose , Pumera Martin 
Published: December 2022

Thiol–ene click chemistry is presented as a “one-pot” synthetic strategy to implant molecular features upon commercially available allyl germanane 2D-Ge–CH2CHCH2 by covalently grafting thiol-rich active molecular components (R′–SH).
The Challenges Encountered by Conventional Hearing Aid and/or Cochlear Implant Users During the COVID-19 Pandemic
Authors: Kabiş Burak , Yıldırım Gökay Nuriye , Tutar Hakan , Karamert Recep , Gündüz Bülent 
Published: December 2022

Purpose:
The purpose of this study was to evaluate the stress, quality of life, and access to hearing health services experienced by adults and children who use hearing aid and/or cochlear implant and their families during the COVID-19 pandemic.

Method:
Perceived Stress Scale, Personal Wellbeing Index, and World Health Organization Quality of Life questionnaires were applied online to 30 adults with hearing loss and parents of 61 children with hearing loss. Also, a survey was conducted to acquire more about the demographics of people who use cochlear implants and conventional hearing aids, as well as the difficulties they encountered during the COVID-19 period.

Results:

Although child user families experienced more problems than adults during the COVID-19 period, the stress levels of child user families were higher than that of adult user families (
p
= .05). Even though there was no statistically significant difference, the families of pediatric users had lower quality of life than adult users.

Conclusions:
The stress/anxiety problems that arise as a result of this are coming to the forefront of both patients and their relatives. The findings indicate that creative strategies should be developed to give individuals with hearing loss efficient access to hearing health services.
“Communication Is Not Exactly My Field, but It Is Still My Area of Work”: Staff and Managers' Experiences of Communication With People With Traumatic Brain Injury
Authors: Christensen Iben , Power Emma , Togher Leanne , Norup Anne 
Published: December 2022

Purpose:
Communication between people with traumatic brain injury (TBI) and rehabilitation staff can be impacted by cognitive-communication disorders. Communication partner training (CPT) programs hold potential to improve communication between people with TBI and their communication partners. To tailor CPT programs to the rehabilitation environment, it is critical to understand the nature of communicative interactions for staff members. This study explores staff and managers' experiences of communication with people with TBI in the rehabilitation setting.

Method:

Individual semistructured videoconference interviews were carried out with staff members (
n
= 22) and managers (
n
= 4) varying in terms of professions and length of work experience. Interviews were guided by a phenomenological approach to gain comprehensive knowledge of staff and managers' lived experiences of communication with people with TBI.

Results:
Four themes with nine subthemes were generated: (a) cognitive-communication disorders challenge interaction (communication is socially inappropriate, the diversity of presenting symptoms associated with TBI is demanding), (b) staff struggle in communication (there is a breakdown of communicative collaboration, communication increases staff's workload, emotional well-being of staff is at risk), (c) communication context is crucial (tasks affect communication, extending the professional roles), and (d) staff want communication knowledge and skills (needs vary according to work experience, access to tailored strategies, and feedback).

Conclusions:
Staff and managers experienced communication with people with TBI as highly demanding. The socially inappropriate communication in people with TBI was perceived to disturb the collaborative aspects of communication and create negative emotional impacts for staff. Staff experiences varied according to discipline, work experience, rehabilitation tasks, and facility. Consequently, staff and managers expressed a need for specific communication strategies to help them overcome the communicative challenges.

Supplemental Material:

https://doi.org/10.23641/asha.21579159
Validation of the Vanderbilt Responses to Your Child's Speech Rating Scale for Parents of Young Children Who Stutter
Authors: Singer Cara M. , Kelly Ellen M. , White A. Zebedee , Zengin-Bolatkale Hatun , Jones Robin M. 
Published: December 2022

Purpose:
This study reports the development, refinement, and exploration of the psychometric properties of the Vanderbilt Responses to Your Child's Speech (VRYCS) rating scale, a novel measure designed to assess parents' self-perceived responses to the speech of their children who stutter (CWS).

Method:
Parents of 214 young (ages 2;4[years;months]–5;11) CWS completed the original 40-item version of the VRYCS. Content analyses and an exploratory factor analysis determined the scale's factors and identified specific items corresponding to each. Items that did not load onto the factors were removed and internal consistency was assessed.

Results:
The final 18-item VRYCS rating scale includes five factors relating to parents' responses to the speech of their CWS including (a) Requesting Change, (b) Speaking for the Child, (c) Supporting Communication, (d) Slowing and Simplifying, and (e) Responding Emotionally. Reliability was demonstrated, responses by parents of CWS were described, and a revised scoring form developed.

Conclusions:
The VRYCS is a valid and reliable tool for assessing and exploring parents' perceptions of their own responses to the speech of their CWS for clinical and research purposes. Clinical applications of the VRYCS for assessment and treatment of childhood stuttering are discussed.
Production and Comprehension of Prosodic Boundary Marking in Persons With Unilateral Brain Lesions
Authors: de Beer Carola , Hofmann Andrea , Regenbrecht Frank , Huttenlauch Clara , Wartenburger Isabell , Obrig Hellmuth , Hanne Sandra 
Published: December 2022

Purpose:

Persons with unilateral brain damage in the right hemisphere (RH) or left hemisphere (LH) show limitations in processing linguistic prosody, with yet inconclusive results on their ability to process prosodically marked structural boundaries for syntactic ambiguity resolution. We aimed at systematically investigating production and comprehension of three prosodic cues (
f
0
range, final lengthening, and pause) at structural boundaries in coordinate sequences in participants with right hemisphere brain damage (RHDP) and participants with left hemisphere brain damage (LHDP).

Method:
Twenty RHDP and 15 LHDP participated in our study. Comprehension experiment: Participants and a control group listened to coordinate name sequences with internal grouping by a prosodically marked structural boundary (grouped condition, e.g., “(Gabi und Leni) # und Nina”) or without internal grouping (ungrouped condition, e.g., “Gabi und Leni und Nina”) and had to identify the target condition. The strength and combinations of prosodic cues in the stimuli were manipulated. Production experiment: Participants were asked to produce coordinate sequences in the two conditions (grouped, ungrouped) in two different tasks: a Reading Aloud and a Repetition experiment. Accuracy of participants' productions was subsequently assessed in a rating study and productions were analyzed with respect to use of prosodic cues.

Results:

In the Comprehension experiment, RHDP and LHDP had overall lower identification accuracies than unimpaired control participants and LHDP were found to have particular problems with boundary identification when the pause cue was reduced. In production, LHDP and RHDP employed all three prosodic cues for boundary marking, but struggled to clearly mark prosodic boundaries in 28% of all productions. Both groups showed better performance in reading aloud than in repetition. LHDP relied more on using
f
0
range and pause duration to prosodically mark structural boundaries, whereas RHDP employed final lengthening more vigorously than LHDP in reading aloud.

Conclusions:
We conclude that processing of linguistic prosody is affected in RHDP and LHDP, but not completely impaired. Therefore, prosody can serve as a relevant communicative resource. However, it should also be considered as a target area for assessment and treatment in both groups.

Supplemental Material:

https://doi.org/10.23641/asha.21637505
Toward Neurodiversity: How Conversation Analysis Can Contribute to a New Approach to Social Communication Assessment
Authors: Yu Betty , Sterponi Laura 
Published: December 2022

Purpose:
The purpose of this clinical focus article was to illustrate the potential of employing conversation analysis (CA) as a method for assessing social communication that is neurodiversity affirming.

Method:
This clinical focus article will provide an overview of CA and explain how it offers a theoretically grounded means of analyzing autistic children's everyday social interactions. Our aim is not simply to add a new assessment instrument to the disciplinary toolbox but to use the occasion to spur a reconsideration of how social communicative competence is currently conceptualized in the field and how those assumptions are reified through assessment practices. We will present a case illustration of a bilingual autistic child and his family. We will discuss the implications of a CA-informed assessment for reconceptualizing autistic social communicative competence.

Results:
The case study illustrates the contributions of CA for (a) shifting the focus of assessment from social communication as an individual skill to social communication as an interactional achievement and (b) surfacing social communicative competencies that may be dismissed as pathologies.

Conclusions:
CA offers a relational understanding of autistic communication and sociality that is compatible with a critical stance on disability. Insights from CA problematize deeply entrenched notions of autism and social communication in speech-language pathology.
Spatio‐temporal decision‐making and trajectory planning framework with flexible constraints in closed‐loop dynamic traffic
Authors: Zhang Ting , Fu Mengyin , Liu Tong , Song Wenjie 
Published: December 2022

AbstractDriving in interactive dynamic traffic is a huge challenge for autonomous vehicles, especially for motion planning. The autonomous vehicle not only needs to predict the future states of the social vehicles to avoid a collision but also realizes the comfort and continuous plan. To deal with the problem, a spatio‐temporal decision‐making and motion planning framework with flexible constraints based on previous work is proposed. Improvements can be highlighted in three aspects. First, a neural network for trajectory prediction is trained and it is integrated into the framework, which can learn the social vehicles' reactions in the negotiation better and give more accurate predictions. Second, instead of setting the fixed local targets, the flexible‐constraint mechanism is introduced to increase the success rate of continuous planning, specifically, the local planning target is determined conditioned on the situation with an elastic factor. Third, two popular simulators with randomly disposed traffic participants are exploited, where vehicles are mutually influenced, more similar to real‐world traffic. The closed‐loop tests with zero crashes and the comparative experiments with traditional models demonstrate the superior performance and practical significance of the proposed framework.