bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensory preference criteria and willingness to adopt vegetable soybean “Edamame” in Benin (West Africa)
Authors: Ahomondji Symphorien Essèdjlo , Agoyi Eric Etchikinto , Agbangba Codjo Emile , Lokonon Bruno Enagnon , Agbahoungba Symphorien , Assogbadjo Achille Ephrem , Sinsin Brice 
Published: March 2022

Abstract
Edamame, has many nutritional and human health benefits and is likely to overcome malnutrition, a common scourge among West African populations. This study aims to identify sensory preference criteria in order to select edamame varieties that meet the organoleptic needs of potential consumers in Benin. Ten soybean varieties were selected on the basis of their stability, adaptability and yield performance in Benin. These varieties were evaluated through hedonic test conducted with 200 untrained panelists, followed by a descriptive sensory evaluation conducted with 20 trained panelists. The hedonic test used sensory attributes such as appearance, flavor, taste, and texture. The study showed significant variation in sensory attributes for most genotypes (P < 0.001), expressing the variation in choices made by the panelists. The genotypes AGS 466, AGS 429, AGS 472, and Ashorowasse were the most preferred (more than 90%). Taking gender into account, AGS 466 and Ashorowasse were most preferred by women, who mainly based their choice on seed appearance and sweetness. In opposite, men were more interested in the taste and texture. Soybean varieties Maksoy 3N and Early hakucho were least preferred (less than 20%) by women and men, respectively. Respondents expressed a high willingness (90.11%) to adopt edamame. Preference criteria were large seed size, sweet taste, moderate chewiness, and starchy texture. The high-yielding grain-type soybean variety S1079/6/7 that showed moderate acceptability for edamame consumption among consumers in Benin may be considered by breeders for improvement in sensory attributes.
Delay Discounting, Probability Discounting, and Interdental Cleaning Frequency
Authors: DeFulio Anthony , Rzeszutek Mark 
Published: March 2022

Abstract
Background: Interdental cleaning is recommended by dentists but many people do not floss regularly. The health benefits of interdental cleaning are delayed, and sensitivity to delay is an important factor in many health behaviors. Thus, the present studies explore the relationship between frequency of flossing, and sensitivity to delayed and probabilistic outcomes. Method: Crowd-sourced participants were recruited in two studies (n = 584 and n = 321, respectively). In both studies, participants reported their frequency of flossing and completed delay discounting and probability discounting tasks. Discounting was measured with Area Under the Curve, and linear regression was used to analyze the results. Results: Findings show that higher levels of delay discounting were associated with less frequent flossing (p < .001, both studies). In contrast, probability discounting was not significantly associated with flossing frequency (ns, both studies). Conclusion: The findings are consistent with prior studies involving other health behaviors such as attendance at primary care and medication adherence. Results suggest that interventions that reduce delay discounting may help promote regular interdental cleaning, and that delay discounting is a more robust predictor of health behaviors than probability discounting. In addition, interdental cleaning appears to be a reasonable target behavior for evaluating potentially generalizable behavioral health interventions. Thus, interventions that are successful in promoting oral health behaviors should be considered as candidates for evaluation in other health behavior domains.
Gene expression and post-gene expression influence of Spondias mombin (Hog plum) and Nigella sativum (Black seed) on diabetogenic Wistar rats.
Authors: Eluehike Nkeiruka , AGU Kingsley Chukwunonso , Oriakhi Kelly , Onoagbe Iyere 
Published: March 2022

Abstract
Background: Diabetes Mellitus (DM) is a metabolic disorder characterised by chronic hyperglycaemia due to the body’s inability to appropriately metabolism and utilize carbohydrates (sugars), further affecting protein and lipid metabolism, as a result of inadequate insulin release due to damaged pancreatic beta-cells (type 1 diabetes) or reduced tissue insulin sensitivity and elevated blood insulin (type 2 diabetes) – endocrine disorder. The possible anti-DM properties of Spondias mombin and Nigella sativum where investigated.Materials and methods: Four (4) groups ten (10) adult Wistar rats were induced with streptozotocin (STZ) to cause a pathologic DM condition after which they were treated with metformin (positive control drug, PC), Spondias mombin methanolic extract (T1) and Nigella sativum oil (T4) for twenty-eight days. The normal control (NC) and diabetic control (DC) received distilled water and STZ. Gene expression studies and molecular docking (post-gene expression) analyses were carried out on key factors involved in DM; lipid and glucose homeostasis (peroxisome proliferator-activated receptors, PPAR-γ), transport proteins (GLUT 1 to 5), proteins of inflammatory reactions (interleukin-1-beta, IL-1β; tumour necrosis factor-alpha-1), enzymes involved in glycolysis (phosphofructokinase-1, PFK-1).Results: It was observed that Spondias mombin, Nigella sativum, and metformin positively influenced the gene expression and post-gene expression phases to ameliorate the DM conditions.Conclusion: Spondias mombin performed better than Nigella sativum and metformin as a potential anti-DM plant.
Non-Invasive Evaluation of MAFLD and the Contribution of Genes: An MRI-PDFF-Based Cross-Sectional Study
Authors: Yang Aruhan , Zhu Xiaoxue , Zhang Lei , Zhang Yingwen , Zhang Dezhi , Niu Junqi , Zhang Huimao , Ding Yanhua , Lv Guoyue 
Published: March 2022

Abstract
Objective
To investigate the clinical, laboratory and genetic features of MAFLD patients based on MRI-PDFF and liver biopsy in China.
Design:
Patients with high ALT and with a diagnosis of fatty liver were included in this cross-sectional study. Fasting blood was collected to test biomarkers and SNPs. A total of 266 patients underwent MRI-PDFF and FibroScan examinations, and 38 underwent liver biopsy. Diagnostic models (decision tree, LASSO, and elastic net) were developed based on diagnosis from MRI-PDFF reports.
Results
Approximately 1/3 were found to have NASH and fibrosis. After quantifying liver steatosis by MRI-PDFF (healthy: N = 47; mild MAFLD: N = 136; moderate/severe MAFLD: N = 83; liver fat content (LFC): 3.6% vs. 8.7% vs. 19.0%), most biomarkers showed significant differences among the three groups, and patients without obesity were found to have a similar LFC as those with obesity (11.1% vs. 12.3%). Variant alleles of PNPLA3, HSD17B13 and MBOAT7 were identified as genetic risk factors causing higher LFC (8.7% vs. 12.3%; 11.0% vs. 14.5%; 8.5% vs. 10.2%, P < 0.05); those with the UQCC1 rs878639 variant allele showed lower LFC (10.4% vs. 8.4%; OR = 0.58, P < 0.05). Patients with more risk alleles had higher LFCs (8.1% vs. 10.7% vs. 11.6% vs. 14.5%). Models including biomarkers showed strong diagnostic ability (accuracy: 0.80–0.91).
Conclusions
When the effects of genes on liver steatosis were first quantified by MRI-PDFF, the UQCC1 rs878639 G allele was identified as a protective factor, and the MBOAT7 T allele was identified as a risk only among nonobese individuals. Machine learning-based models can accurately predict disease status.
The Regulation of Insulin Receptor/insulin-like Growth Factor Receptor Ratio, an Important Factor for Breast Cancer Prognosis, by TRIP-Br1
Authors: Quynh Nguyen Thi Ngoc , Jung Samil , Anh Nguyen Hai , Lee Beom Suk , Myagmarjav Davaajargal , Eum Hye Hyeon , Lee Hae-Ock , Jo Taeyeon , Mongre Raj Kumar , Choi Yeongseon , Lee Myeong-Sok 
Published: March 2022

Abstract
Much higher risk of cancer is observed in patients with diabetes. Insulin receptor (IR) and insulin-like growth factor-1 receptor (IGF1R) are well-known targets in cancer research as well as diabetes treatment. Interestingly, a recent study proposed that the IR/IGF1R ratio is an important factor in breast cancer prognosis. Women with a higher IR/IGF1R ratio showed poor breast cancer prognosis and hyperinsulinemia. Here, we propose a novel mechanism by which the oncogenic protein TRIP-Br1 renders breast cancer cells to have a higher IR/IGF1R ratio by positively and negatively regulating IR and IGF1R expression at the protein level, respectively. TRIP-Br1 plays various cellular roles, one of which is as an adaptor protein. Our data revealed that TRIP-Br1 suppresses ubiquitin/proteasome-mediated IR degradation without directly interacting with IR. Meanwhile, TRIP-Br1 directly interacts with both IGF1R and NEDD4-1 E3 ubiquitin ligase, and TRIP-Br1/NEDD4-1 degrades IGF1R via the ubiquitin/proteasome system. Animal experiments indicated that TRIP-Br1 enhanced tumor progression, where a high IR/IGF1R ratio was detected. Furthermore, IR silencing elevates IGF1R expression, resulting in a lower IR/IGF1R ratio. Our extended study showed a similar effect of TRIP-Br1 on the IR/IGF1R ratio in insulin-deficient mice mimicking patients with diabetes, confirming the strong relationship between breast cancer and diabetes. In conclusion, this study provides invaluable information on the regulatory mechanism of how breast cancer cells acquire a higher IR/IGF1R ratio.
Standard Setting Very Short Answer Questions (VSAQs) Relative to Single Best Answer Questions (SBAQs): Does Having Access to the Answers Make a Difference?
Authors: Sam Amir H , Millar Kate R , Westacott Rachel , Melville Colin R , Brown Celia A 
Published: March 2022

Abstract
BackgroundWeinvestigated whether question formatand access to the correct answers affect the pass mark set by standard-setters on written examinations. MethodsTrained educatorsused the Angoff method to standard set two 50-item tests with identical vignettes, one in a single best answer question (SBAQ) format (with five answer options) and the other in a very short answer question (VSAQ) format (requiring free text responses). Half the participants had access to the correct answers and half did not. The data for each group were analysed to determine if the question format or having access to the answers affected the pass mark set.ResultsA lower pass mark was set for the VSAQ test than the SBAQ testby the standard setters who had access to the answers (median difference of 13.85 percentage points, Z=-2.82, p=0.002). Comparable pass marks were set for the SBAQ test by standard setters with and without access to the correct answers (60.65% and 60.90% respectively).A lower pass mark was set for the VSAQ testwhen participants hadaccess to the correct answers (difference in medians -13.75 percentage points, Z=2.46, p=0.014).ConclusionsWhen given access to the potential correct answers, standard setters appear to appreciate the increased difficulty of VSAQs compared to SBAQs.
Analysis for MBOAT Family Genes in Human Liver Hepatocellular Carcinoma
Authors: Wang Quanjun , Wang Minghan , Han Junyuan , Jiang Ying 
Published: March 2022

Abstract
Membrane-bound O-acyltransferases (MBOATs), a group of integral transmembrane enzymes that perform divergent functions which are attractive drug targets in cancer. 11 of MBOAT proteins (MBOAT1, MBOAT2, HHATL, GOAT, LPCAT3, MBOAT7, SOAT1, SOAT2, HHAT, DGAT1, PORCN) are located in the progression of various cancer types, especially in Liver Hepatocellular Carcinoma. Although these proteins are important drug targets, the diverse expression patterns and prognostic values have yet to be analyzed. Here we discussed the transcriptional and survival data of 11 of MBOAT proteins in patients with Hepatocellular Carcinoma from the cBioPortal databases, Gene Expression Proﬁling Interactive Analysis (GEPIA), Oncomine, and Kaplan-Meier Plotter. We found that the expression levels of MBOAT2 and 7 were correlated with advanced tumor stage. Survival analysis using the Kaplan- Meier Plotter database revealed that the high transcription levels of MBOAT1, 7, SOAT1 and PORCN were associated with low relapse-free survival in all of the patients with Liver Hepatocellular Carcinoma. Conversely, high HHATL levels predicted high relapse-free survival in these patients. This study implied that MBOAT1, 7, SOAT1 and PORCN are potential targets of precision therapy for patients with Liver Hepatocellular Carcinoma and that HHATL are new biomarkers for the prognosis of Liver Hepatocellular Carcinoma. -ehog acyltransferase (HHAT)11-16. The expression of some MBOAT proteins are deregulated in several human malignancies, including chronic lymphocytic leukemia (CLL), pancreatic ductal adenocarcinoma(PDAC), prostate cancer, clear cell renal carcinoma (ccRCC), Glioblastoma(GBM), breast cancer and liver hepatocellular carcinoma(LIHC)17-23. Liver hepatocellular carcinoma is the the fourth most common cause of cancer-related death worldwide and the leading cause of cancer-related death around the world. Early- stage LIHC is amenable to potentially curative treatment, which includes local ablation, surgical resection and liver transplantation24. LIHC has identified six robust subgroups of , designated G1 – G6 which are associated with specific genetic and clinical characteristics25-26. Mutations in the TERT promoter (occurring in 44 – 65% of patients with LIHC and encodin transcription of the catalytic subunit of telomerase), gregulating CTNNB1 (27–40%, β-catenin, a proto-oncogene in the WNT signalling pathway) and TP53 (21 – 31%, the master cell cycle regulator) are the most conventional27-28. Despite the headways made in liver hepatocellular carcinoma management, including noninvasive radiological diagnosis and more availability treatment options over the past few decades, of which only overall rate of survival after developing this cancer29. Due to the heterogeneity of tumor, biomarkers that predict prognosis at current have some boundedness, thus, new biomarkers as prognostic indicators to availably prognosis and personalized treatment are very important.
PD-L1 Testing In Metastatic Triple Negative Breast Cancer: Results Of An Italian Survey
Authors: Cerbelli Bruna , Cirillo Alessio , Pomati Giulia , Pernazza Angelina , Ascione Andrea , Pisegna Simona , Pisano Annalinda , Leopizzi Martina , Pignataro Maria Gemma , Costarelli Leopoldo , Mulè Antonino , Vecchione Andrea , Catalano Piera , Coppola Luigi , Perrone Giuseppe , Perracchio Letizia , Anemona Lucia , Mastracchio Antonio , Nardi Stefano , Reitano Renato , Massari Annalisa , Grillo Lucia Rosalba , Liberati Fabrizio , Rocca Carlo Della , Marchetti Paolo , Botticelli Andrea , D’Amati Giulia 
Published: March 2022

Abstract
Background: Immunotherapy has revolutionized the approach to metastatic triple-negative breast cancers (mTNBC). Atezolizumab was approved for patients with mTNBC whose tumours express PD-L1, determined by SP 142 assay. To assess the availability and practice of SP142 test we administered a survey to all the 15 Pathology Departments of the Lazio Region during a 6-months period.Methods: The survey comprised 12 questions regarding the availability of SP142 in the Pathology Departments, the percentage of positive tests, the difficulties of pathologists in case close to cut-off value and the tested samples.Results: The SP142 assay was available only in 8 Centers. In case of positive result, most Centers (5/8, 62.5%) reported values of PD-L1 expression ranging from > 1 to <= 5%, with values close to the cut-off point (≥ 1% or < 1%) being the greatest challenge. Most of Centers (6/8, 75%) tested material from both their own and other Hospitals. In most Centers, the evaluations were performed either on primary tumors or metastasis, in particular lymph nodes (5/8, 62.5%), followed by lung (3/8, 37.5%) and liver (1/8, 12.5%) metastasis. ﻿Conclusion: Our results raise some important issues concerning the evaluation of PD-L1 in the “real-life” setting, providing strategies for its implementation.
Morphological Features of Bronchiectasis in Patients With Non-tuberculous Mycobacteriosis and Interstitial Pneumonia
Authors: Tabe Chiori , Dobashi Masaki , Ishioka Yoshiko , Itoga Masamichi , Tanaka Hisashi , Taima Kageaki , Tasaka Sadatomo 
Published: March 2022

Abstract
Background: The pathogenesis of bronchiectasis differs between chronic lower respiratory tract infection and interstitial pneumonia. However, there has been no report that compared their morphological characteristics by quantitative analysis of high-resolution computed tomography (HRCT) images.Objective: We aimed to compare the morphological features of bronchiectasis between patients with different underlying diseases.Method: Fourteen patients with non-tuberculous mycobacteriosis (NTM) and 13 with idiopathic pulmonary fibrosis (IPF) were included. A 3D image of the bronchial structure was made from HRCT data using Ziostation 2 (Ziosoft, Inc., Tokyo, Japan). Bronchiectasis was defined as abnormal dilatation of the bronchi with the diameter greater than that of the accompanying pulmonary artery. We measured the inner and outer diameters, wall area as %total airway cross sectional area (WA%), and wall thickness to airway diameter ratio (T/D) of the 4-8th generations of bronchi. Result: In patients with IPF, the inner and outer diameters linearly decreased toward the distal bronchi. In contrast, the inner and outer diameters of NTM fluctuated. The coefficient of variation of the outer diameters of the 6-7th generations of bronchi was larger in the NTM patients than in those with IPF, whereas no significant difference was observed in the coefficient of variation of the inner diameters between the groups. In IPF patients, WA% and T/D varied between the generation of bronchi, but the coefficient of variation of WA% and T/D was relatively small in those with NTM.Conclusion: The morphological characteristics of bronchiectasis were different between the IPF patients and those with NTM, which might be associated with the pathogenesis of bronchiectasis and edematous changes of the bronchial walls.
Prognosis and Treatment of Asymptomatic Versus Symptomatic Metastatic Breast Cancer: A Multicenter Retrospective Study
Authors: Kuba Sayaka , Maeda Shigeto , Minami Shigeki , Moriuchi Hiroki , Tanaka Aya , Akashi Momoko , Morita Michi , Sakimura Chika , Baba Masayuki , Otsubo Ryota , Matsumoto Megumi , Yamanouchi Kosho , Yano Hiroshi , Kanetaka Kengo , Nagayasu Takeshi , Eguchi Susumu 
Published: March 2022

Abstract
Background
In Japan, asymptomatic metastatic breast cancer (MBC) is often detected using any tumor marker or imaging tests. We aimed to determine whether differences in clinicopathological features, subtypes, prognosis, and treatment exist between asymptomatic and symptomatic MBCs.
Methods
We performed a multicenter, retrospective analysis of patients with MBC who visited our hospitals from 2008 to 2018. Patients were divided into asymptomatic and symptomatic groups to compare their prognosis by breast cancer subtypes: luminal (hormone receptor positive/hormone receptor negative/human epidermal growth factor receptor 2 (HER2) negative), HER2 (any hormone receptor/HER2 positive), and TN (hormone receptor negative/HER2 negative).
Results
Of 204 patients with MBC (114 asymptomatic, 90 symptomatic), the symptomatic group had a higher frequency of multiple metastatic sites and TN subtype. All cohorts and TN patients in the asymptomatic group had longer post-recurrence survival (all cohorts: p < 0.001, TN: p = 0.01) and tended to have longer overall survival (all cohorts: p = 0.09, TN: p = 0.06) than those in the symptomatic group. In contrast, for the luminal and HER2 subtypes, post-recurrence survival was higher in the asymptomatic group than in the symptomatic group (luminal: p = 0.08, HER2: p = 0.09) but without significant difference in overall survival. In the multivariate analysis, TN, recurrence-free survival, multiple metastatic sites, and symptomatic MBC were independently predictive of post-recurrence survival. Regarding the luminal subtype, the asymptomatic group had longer durations of chemotherapy than the symptomatic group, with no significant difference observed in overall survival between the groups.
Conclusion
Asymptomatic and symptomatic MBCs require unique treatments due to prognostic differences.
Human Integrin α10β1-Selected Mesenchymal Stem Cells Home To Cartilage Defects In The Rabbit Knee And Assume A Chondrocyte-Like Phenotype
Authors: Andersen Camilla , Uvebrant Kristina , Mori Yuki , Aarsvold Stacie , Jacobsen Stine , Berg Lise Charlotte , Lundgren-Åkerlund Evy , Lindegaard Casper 
Published: March 2022

Abstract
Background: Mesenchymal stem cells (MSCs) have shown promising results in stimulating cartilage repair and in the treatment of osteoarthritis (OA). However, the fate of the MSCs after intra-articular injection and their role in cartilage regeneration is not clear. To address these questions, this study investigated 1) homing of labeled human adipose tissue derived integrin α10β1-selected MSCs (integrin α10-MSCs), to a cartilage defect in a rabbit model and 2) the ability of the integrin a10-MSCs to differentiate to chondrocytes and to produce cartilage matrix molecules in vivo.Design: Integrin α10-MSCs were labeled with superparamagnetic iron oxide nanoparticles (SPIONs) co-conjugated with Rhodamine-B to allow visualization by both MRI and fluorescence microscopy. A cartilage defect was created in the articular cartilage of the intertrochlear groove of the femur of rabbits. Seven days post-surgery, labeled integrin α10-MSCs or vehicle were injected into the joint. Migration and distribution of the SPION-labeled integrin α10-MSCs was evaluated by high field 9.4T MRI up to 10 days after injection. Tissue sections from the repair tissue in the defects were examined by fluorescence microscopy.Results: In vitro characterization of the labeled integrin α10-MSCs demonstrated maintained viability, proliferation rate and tri-lineage differentiation capacity compared to unlabeled MSCs. In vivo MRI analysis detected the labeled integrin α10-MSCs in the cartilage defects at all time points from 12 hours after injection until day 10 with a peak concentration between day 1-4 after injection. The labeled MSCs were also detected lining the synovial membrane at the early time points. Fluorescence analysis confirmed the presence of the labeled integrin α10-MSCs in all layers of the cartilage repair tissue and showed co-localization between the MSCs and the specific cartilage molecules aggrecan and collagen type II indicating in vivo differentiation of the MSCs to chondrocyte-like cells. No adverse effects of the α10-MSC treatment were detected during the study period.Conclusion: Our results demonstrated migration and homing of human integrin α10β1-selected MSCs to cartilage defects in the rabbit knee after intra-articular administration indicating that the MSCs have a direct role in the cartilage regeneration.