bluuebunny/crossref_metadata_2025_split huggingface dataset:
HGF/c-Met pathway facilitates the perineural invasion of pancreatic cancer by activating the mTOR/NGF axis
Authors: Qin Tao , Xiao Ying , Qian Weikun , Wang Xueni , Gong Mengyuan , Wang Qiqi , An Rui , Han Liang , Duan Wanxing , Ma Qingyong , Wang Zheng 
Published: April 2022

AbstractPerineural invasion (PNI) is a pathologic feature of pancreatic cancer and is associated with poor outcomes, metastasis, and recurrence in pancreatic cancer patients. However, the molecular mechanism of PNI remains unclear. The present study aimed to investigate the mechanism that HGF/c-Met pathway facilitates the PNI of pancreatic cancer. In this study, we confirmed that c-Met expression was correlated with PNI in pancreatic cancer tissues. Activating the HGF/c-Met signaling pathway potentiated the expression of nerve growth factor (NGF) to recruit nerves and promote the PNI. Activating the HGF/c-Met signaling pathway also enhanced the migration and invasion ability of cancer cells to facilitate cancer cells invading nerves. Mechanistically, HGF/c-Met signaling pathway can active the mTOR/NGF axis to promote the PNI of pancreatic cancer. Additionally, we found that knocking down c-Met expression inhibited cancer cell migration along the nerve, reduced the damage of the sciatic nerve caused by cancer cells and protected the function of the sciatic nerve in vivo. Taken together, our findings suggest a supportive mechanism of the HGF/c-Met signaling pathway in promoting PNI by activating the mTOR/NGF axis in pancreatic cancer. Blocking the HGF/c-Met signaling pathway may be an effective target for the treatment of PNI.
Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer
Authors: Zhang Jieyun , Liu Fatao , Yang Yanan , Yu Nuoya , Weng Xiaoling , Yang Yue , Gong Zhe , Huang Shenglin , Gan Lu , Sun Sijie , Zhang Xiaowei , Gong Yiwei , Liu Yun , Guo Weijian 
Published: April 2022

AbstractGastric cancer (GC) is the second cause of cancer-related death and metastasis is an important cause of death. Considering difficulties in searching for metastatic driver mutations, we tried a novel strategy here. We conducted an integrative genomic analysis on GC and identified early drivers lead to metastasis. Whole-exome sequencing (WES), transcriptomes sequencing and targeted-exome sequencing (TES) were performed on tumors and matched normal tissues from 432 Chinese GC patients, especially the comparative analysis between higher metastatic-potential (HMP) group with T1 stage and lymph-node metastasis, and lower metastatic-potential (LMP) group without lymph-nodes or distant metastasis. HMP group presented higher mutation load and heterogeneity, enrichment in immunosuppressive signaling, more immune cell infiltration than LMP group. An integrated mRNA-lncRNA signature based on differentially expressed genes was constructed and its prognostic value was better than traditional TNM stage. We identified 176 candidate prometastatic mutations by WES and selected 8 genes for following TES. Mutated TP53 and MADCAM1 were significantly associated with poor metastasis-free survival. We further demonstrated that mutated MADCAM1 could not only directly promote cancer cells migration, but also could trigger tumor metastasis by establishing immunosuppressive microenvironment, including promoting PD-L1-mediated immune escape and reprogramming tumor-associated macrophages by regulating CCL2 through Akt/mTOR axis. In conclusion, GCs with different metastatic-potential are distinguishable at the genetic level and we revealed a number of potential metastatic driver mutations. Driver mutations in early-onset metastatic GC could promote metastasis by establishing an immunosuppressive microenvironment. This study provided possibility for future target therapy of GC.
Curving the space by non-Hermiticity
Authors: Lv Chenwei , Zhang Ren , Zhai Zhengzheng , Zhou Qi 
Published: April 2022

AbstractQuantum systems are often classified into Hermitian and non-Hermitian ones. Extraordinary non-Hermitian phenomena, ranging from the non-Hermitian skin effect to the supersensitivity to boundary conditions, have been widely explored. Whereas these intriguing phenomena have been considered peculiar to non-Hermitian systems, we show that they can be naturally explained by a duality between non-Hermitian models in flat spaces and their counterparts, which could be Hermitian, in curved spaces. For instance, prototypical one-dimensional (1D) chains with uniform chiral tunnelings are equivalent to their duals in two-dimensional (2D) hyperbolic spaces with or without magnetic fields, and non-uniform tunnelings could further tailor local curvatures. Such a duality unfolds deep geometric roots of non-Hermitian phenomena, delivers an unprecedented routine connecting Hermitian and non-Hermitian physics, and gives rise to a theoretical perspective reformulating our understandings of curvatures and distance. In practice, it provides experimentalists with a powerful two-fold application, using non-Hermiticity to engineer curvatures or implementing synthetic curved spaces to explore non-Hermitian quantum physics.
USP22 regulates lipidome accumulation by stabilizing PPARγ in hepatocellular carcinoma
Authors: Ning Zhen , Guo Xin , Liu Xiaolong , Lu Chang , Wang Aman , Wang Xiaolin , Wang Wen , Chen Huan , Qin Wangshu , Liu Xinyu , Zhou Lina , Ma Chi , Du Jian , Lin Zhikun , Luo Haifeng , Otkur Wuxiyar , Qi Huan , Chen Di , Xia Tian , Liu Jiwei , Tan Guang , Xu Guowang , Piao Hai-long 
Published: April 2022

AbstractElevated de novo lipogenesis is considered to be a crucial factor in hepatocellular carcinoma (HCC) development. Herein, we identify ubiquitin-specific protease 22 (USP22) as a key regulator for de novo fatty acid synthesis, which directly interacts with deubiquitinates and stabilizes peroxisome proliferator-activated receptor gamma (PPARγ) through K48-linked deubiquitination, and in turn, this stabilization increases acetyl-CoA carboxylase (ACC) and ATP citrate lyase (ACLY) expressions. In addition, we find that USP22 promotes de novo fatty acid synthesis and contributes to HCC tumorigenesis, however, this tumorigenicity is suppressed by inhibiting the expression of PPARγ, ACLY, or ACC in in vivo tumorigenesis experiments. In HCC, high expression of USP22 positively correlates with PPARγ, ACLY or ACC expression, and associates with a poor prognosis. Taken together, we identify a USP22-regulated lipogenesis mechanism that involves the PPARγ-ACLY/ACC axis in HCC tumorigenesis and provide a rationale for therapeutic targeting of lipogenesis via USP22 inhibition.
Dual-comb photothermal spectroscopy
Authors: Wang Qiang , Wang Zhen , Zhang Hui , Jiang Shoulin , Wang Yingying , Jin Wei , Ren Wei 
Published: April 2022

AbstractDual-comb spectroscopy (DCS) has revolutionized optical spectroscopy by providing broadband spectral measurements with unprecedented resolution and fast response. Photothermal spectroscopy (PTS) with a pump-probe configuration offers a highly sensitive gas sensing method, which is normally performed using a single-wavelength pump laser. The merging of PTS with DCS may enable a spectroscopic method by taking advantage of both technologies, which has never been studied yet. Here, we report dual-comb photothermal spectroscopy (DC-PTS) by passing dual combs and a probe laser through a gas-filled anti-resonant hollow-core fiber, where the generated multi-heterodyne modulation of the refractive index is sensitively detected by an in-line interferometer. As an example, we have measured photothermal spectra of acetylene over 1 THz, showing a good agreement with the spectral database. Our proposed DC-PTS provides opportunities for broadband gas sensing with super-fine resolution and high sensitivity, as well as with a small sample volume and compact configuration.
KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling
Authors: Yu Bo , Su Jun , Shi Qiqi , Liu Qing , Ma Jun , Ru Guoqing , Zhang Lei , Zhang Jian , Hu Xichun , Tang Jianming 
Published: April 2022

AbstractSmad nuclear-interacting protein 1 (SNIP1) is a transcription repressor related to the TGF-β signaling pathway and associates with c-MYC, a key regulator of cell proliferation and tumor development. Currently, the mechanism by which SNIP1 regulates tumorigenesis and cancer metastasis is unknown. Here, we identify that SNIP1 is a non-histone substrate of lysine methyltransferase KMT5A, which undergoes KMT5A-mediated mono-methylation to promote breast cancer cell growth, invasion and lung metastasis. Mechanistically, we show KMT5A-mediated K301 methylation of SNIP1 represents a sensing signal to release histone acetyltransferase KAT2A and promotes the interaction of c-MYC and KAT2A, and the recruitment of c-MYC/KAT2A complex to promoter of c-MYC targets. This event ultimately inhibits the Hippo kinase cascade to enhance triple-negative breast cancer (TNBC) metastasis by transcriptionally activating MARK4. Co-inhibition of KMT5A catalytic activity and YAP in TNBC xenograft-bearing animals attenuates breast cancer metastasis and increases survival. Collectively, this study presents an KMT5A methylation-dependent regulatory mechanism governing oncogenic function of SNIP1.
Strong pairing in mixed-dimensional bilayer antiferromagnetic Mott insulators
Authors: Bohrdt Annabelle , Homeier Lukas , Bloch Immanuel , Demler Eugene , Grusdt Fabian 
Published: April 2022

AbstractInteracting many-body systems in reduced-dimensional settings, such as ladders and few-layer systems, are characterized by enhanced quantum fluctuations. Recently, two-dimensional bilayer systems have sparked considerable interest because they can host unusual phases, including unconventional superconductivity. Here we present a theoretical proposal for realizing high-temperature pairing of fermions in a class of bilayer Hubbard models. We introduce a general and highly efficient pairing mechanism for mobile charge carriers in doped antiferromagnetic Mott insulators. The pairing is caused by the energy that one charge gains when it follows the path created by another charge. We show that this mechanism leads to the formation of highly mobile but tightly bound pairs in the case of mixed-dimensional Fermi–Hubbard bilayer systems. This setting is closely related to the Fermi–Hubbard model believed to capture the physics of copper oxides, and can be realized in currently available ultracold atom experiments.
Recombinant Fsh and Lh therapy for spawning induction of previtellogenic and early spermatogenic arrested teleost, the flathead grey mullet (Mugil cephalus)
Authors: Ramos-Júdez Sandra , Giménez Ignacio , Gumbau-Pous Josep , Arnold-Cruañes Lucas Stephen , Estévez Alicia , Duncan Neil 
Published: April 2022

AbstractWith the expansion and diversification of global aquaculture, efforts continue to develop new bio-technologies for assisted reproduction in species that present reproductive dysfunctions. Flathead grey mullet (Mugil cephalus) males held in intensive conditions in the Mediterranean region do not produce fluent milt and most females are arrested at previtellogenesis. The weekly injections of recombinant follicle stimulating hormone (rFsh) and luteinizing hormone (rLh) induced and completed vitellogenesis in treated females (n = 21), and treated males produced fluent sperm (n = 9). The application of a priming dose of 30 µg kg−1 rLh and resolving dose of 40 mg kg−1 Progesterone, or priming and resolving doses of 30 µg kg−1 rLh, resulted in the induction of maturation, ovulation, and spontaneous spawns with a spawning success of the 85% (8 of 9 females) and 100% (n = 6), respectively. The eggs collected had 63 ± 21% fertilization with embryo development and 58 ± 23% hatching. In comparison, control individuals did not show advances in gonadal development and did not produce fluent sperm. The present results confirm the possibility of controlling oogenesis from previtellogenesis to the completion of maturation and fertilised tank spawning using exclusively rFsh and rLh in a teleost species.
Gene profiling of SEC13, SMAD7, GHRL, long non-coding RNA GHRLOS, HIF-1α in gastric cancer patients
Authors: Hussein Neveen A. , Rashad Mona M. , Abdou Azza S. , Hussein Amr M. , Mohamed Hagar M. 
Published: April 2022

AbstractEven with considerable progress in cancer researches, gastric cancer is still one of the global health problems. Recognition of the differential expressed genes in GC is the most appropriate approach for establishing new diagnostic targets. This study evaluates SEC13, SMAD7, GHRL, lncRNA GHRLOS, HIF-1α genes profiling as well as HIF-1α protein level for GC. The expression of selected genes, serum HIF-1α and CEA protein levels were determined for 50 GC patients and 50 healthy controls by real-time RT-PCR, ELISA, and ELICA respectively. The sensitivities of these parameters as diagnostic biomarkers were evaluated. SMAD7, HIF-1α expression, serum HIF-1α, and CEA level were significantly upregulated in GC patients as compared to the control group (P = 0.024, < 0.001) and had significant positive correlations between each other except SMAD7 with serum HIF-1α, and CEA level. On the other hand, SEC13, GHRL, and lncRNA GHRLOS expression were significantly downregulated in GC patients (P =  < 0.001, 0.025, < 0.001 respectively) and had significant positive correlations with each other (P < 0.001). Significant negative correlations were observed between most of both groups. All studied parameters were associated with GC clinical stages except SMAD7 was associated with stage IV only (P = 0.005) and GHRL did not associate with tumor stages (P ˃ 0.05). All studied parameters may be promising biomarkers for the early diagnosis of GC. SMAD7, HIF-1α gene, and HIF-1α protein may be jointly implicated in cancer development and prognosis, while SEC13, GHRL, and lncRNA GHRLOS may act as tumor suppressors.
Clinical diagnosis of metabolic disorders using untargeted metabolomic profiling and disease-specific networks learned from profiling data
Authors: Thistlethwaite Lillian R. , Li Xiqi , Burrage Lindsay C. , Riehle Kevin , Hacia Joseph G. , Braverman Nancy , Wangler Michael F. , Miller Marcus J. , Elsea Sarah H. , Milosavljevic Aleksandar 
Published: April 2022

AbstractUntargeted metabolomics is a global molecular profiling technology that can be used to screen for inborn errors of metabolism (IEMs). Metabolite perturbations are evaluated based on current knowledge of specific metabolic pathway deficiencies, a manual diagnostic process that is qualitative, has limited scalability, and is not equipped to learn from accumulating clinical data. Our purpose was to improve upon manual diagnosis of IEMs in the clinic by developing novel computational methods for analyzing untargeted metabolomics data. We employed CTD, an automated computational diagnostic method that “connects the dots” between metabolite perturbations observed in individual metabolomics profiling data and modules identified in disease­specific metabolite co-perturbation networks learned from prior profiling data. We also extended CTD to calculate distances between any two individuals (CTDncd) and between an individual and a disease state (CTDdm), to provide additional network-quantified predictors for use in diagnosis. We show that across 539 plasma samples, CTD-based network-quantified measures can reproduce accurate diagnosis of 16 different IEMs, including adenylosuccinase deficiency, argininemia, argininosuccinic aciduria, aromatic l-amino acid decarboxylase deficiency, cerebral creatine deficiency syndrome type 2, citrullinemia, cobalamin biosynthesis defect, GABA-transaminase deficiency, glutaric acidemia type 1, maple syrup urine disease, methylmalonic aciduria, ornithine transcarbamylase deficiency, phenylketonuria, propionic acidemia, rhizomelic chondrodysplasia punctata, and the Zellweger spectrum disorders. Our approach can be used to supplement information from biochemical pathways and has the potential to significantly enhance the interpretation of variants of uncertain significance uncovered by exome sequencing. CTD, CTDdm, and CTDncd can serve as an essential toolset for biological interpretation of untargeted metabolomics data that overcomes limitations associated with manual diagnosis to assist diagnosticians in clinical decision-making. By automating and quantifying the interpretation of perturbation patterns, CTD can improve the speed and confidence by which clinical laboratory directors make diagnostic and treatment decisions, while automatically improving performance with new case data.
Comparison of sequential CT arterioportography-arteriosplenography with standard cross-sectional imaging and endoscopy in children with portal hypertension
Authors: Hammer Simone , Zeman Florian , Schlitt Hans Jürgen , Stroszczynski Christian , Greiner Barbara , Doppler Michael Christian , Uller Wibke 
Published: April 2022

AbstractIn this study the diagnostic capability and additional value of sequential CT arterioportography–arteriosplenography (CT AP–AS) in comparison to standard cross-sectional imaging and upper gastrointestinal endoscopy (UGE) in pediatric portal hypertension (PH) was analyzed. Patients with clinical signs of PH who underwent CT AP–AS in combination with additional contrast-enhanced magnetic resonance imaging (CE-MR) and/or contrast-enhanced computed tomography (CE-CT) were included. Two radiologists reviewed independently imaging regarding the capability to prove patency of (1) extrahepatic and intrahepatic main stem portal vein (PV), (2) intrahepatic PV system and (3) splenomesenteric venous axis. Imaging was reviewed for detection of abdominal varices and results were compared to UGE. Main venous supply of varices (PV and/or splenic vein system) and splenorenal shunting were evaluated. 47 imaging studies (20 CT AP-AS, 16 CE-MR, 11 CE-CT) and 12 UGE records of 20 patients were analyzed. CT AP–AS detected significantly more splenorenal shunts (p = 0.008) and allowed more confident characterization of the extra-/intrahepatic PV-system and splenomesenteric veins in comparison to CE-MR (p < 0.001). Extra- and intrahepatic PV-system were significantly more confidently assessed in CT AP–AS than in CE-CT (p = 0.008 and < 0.001 respectively). CT AP–AS was the only modality that detected supply of varices and additional gastric/duodenal varices. In this retrospective study CT AP–AS was superior to standard cross-sectional imaging concerning confident assessment of the venous portosplenomesenteric axis in pediatric patients. CT AP–AS detected additional varices, splenorenal shunting and supply of varices.
Transient elastography and serum markers of liver fibrosis associate with epicardial adipose tissue and coronary artery calcium in NAFLD
Authors: Perdomo Carolina M. , Ezponda Ana , Núñez-Córdoba Jorge M. , Herrero José I. , Bastarrika Gorka , Frühbeck Gema , Escalada Javier 
Published: April 2022

AbstractNon-alcoholic fatty liver disease (NAFLD) is associated with cardiovascular disease morbimortality. However, it is not clear if NAFLD staging may help identify early or subclinical markers of cardiovascular disease. We aimed to evaluate the association of liver stiffness and serum markers of liver fibrosis with epicardial adipose tissue (EAT) and coronary artery calcium (CAC) in an observational cross-sectional study of 49 NAFLD patients that were seen at Clínica Universidad de Navarra (Spain) between 2009 and 2019. Liver elastography and non-invasive fibrosis markers were used to non-invasively measure fibrosis. EAT and CAC, measured through visual assessment, were determined by computed tomography. Liver stiffness showed a direct association with EAT (r = 0.283, p-value = 0.049) and CAC (r = 0.337, p-value = 0.018). NAFLD fibrosis score was associated with EAT (r = 0.329, p-value = 0.021) and CAC (r = 0.387, p-value = 0.006). The association of liver stiffness with CAC remained significant after adjusting for metabolic syndrome features (including carbohydrate intolerance/diabetes, hypertension, dyslipidaemia, visceral adipose tissue, and obesity). The evaluation of NAFLD severity through liver elastography or non-invasive liver fibrosis biomarkers may contribute to guide risk factor modification to reduce cardiovascular risk in asymptomatic patients. Inversely, subclinical cardiovascular disease assessment, through Visual Scale for CAC scoring, may be a simple and effective measure for patients with potential liver fibrosis, independently of the existence of other cardiovascular risk factors.
Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFβ/SMAD pathway
Authors: Salem Mohamed A. , Manaa Eman G. , Osama Nada , Aborehab Nora M. , Ragab Mai F. , Haggag Yusuf A. , Ibrahim Magda T. , Hamdan Dalia I. 
Published: April 2022

AbstractAging has become a concern for many people, especially women. Given that high-quality anti-aging products are of high cost; it has imperative to search for other economical sources. Essential oils are frequently used in cosmetics products due to a wide range of biological activities as well as their pleasant odor. The current study aimed to investigate the biochemical effect of the cosmetic potential of selected Apiaceous essential oils, traditionally used for skincare, by evaluating their anti-wrinkle activity. It is worth noting that, coriander essential oil showed the highest collagenase, elastase, tyrosinase, and hyaluronidase inhibitory activities compared to other Apiaceous oils (fennel, anise, and cumin). GC–MS proved that coriander essential oil showed a very high level of oxygenated monoterpenes, with linalool (81.29%) as the most abundant constituent. Intriguingly, coriander oil cream and Coriander Essential Oil-loaded Lipid Nanoparticles (CEOLNs) formulations attenuated in vivo UV-induced skin photoaging that was manifested by significantly decreased MDA, COX-2, PGE-2, MMP-1, JNK, and AP-1 levels. Moreover, these pharmaceutical dosage forms significantly increased skin collagen content compared to UV-injured group. Also, coriander essential oil significantly increased TGFβ, TGFβII, and SMAD3 protein expression levels compared to UV-injured group. In conclusion, the pharmaceutical dosage forms of coriander oil possess anti-wrinkle activities that could have an auspicious role in amending extrinsic aging.
One-step preparation of RGO/Fe3O4–FeVO4 nanocomposites as highly effective photocatalysts under natural sunlight illumination
Authors: Alsulami Qana A. , Rajeh A. , Mannaa Mohammed A. , Albukhari Soha M. , Baamer Doaa F. 
Published: April 2022

AbstractThe study used a one-step hydrothermal method to prepare Fe3O4–FeVO4 and xRGO/Fe3O4–FeVO4 nanocomposites. XRD, TEM, EDS, XPS, DRS, and PL techniques were used to examine the structurally and morphologically properties of the prepared samples. The XRD results appeared that the Fe3O4–FeVO4 has a triclinic crystal structure. Under hydrothermal treatment, (GO) was effectively reduced to (RGO) as illustrated by XRD and XPS results. UV–Vis analysis revealed that the addition of RGO enhanced the absorption in the visible region and narrowed the band gap energy. The photoactivities of the prepared samples were evaluated by degrading methylene blue (MB), phenol and brilliant green under sunlight illumination. As indicated by all the nanocomposites, photocatalytic activity was higher than the pure Fe3O4–FeVO4 photocatalyst, and the highest photodegradation efficiency of MB and phenol was shown by the 10%RGO/Fe3O4–FeVO4. In addition, the study examined the mineralization (TOC), photodegradation process, and photocatalytic reaction kinetics of MB and phenol.