bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of Lifestyle during Covid-19 Epidemic in Nursing Students of Yazd Shahid Sadoughi University of Medical Sciences in 2020
Authors: Salmani Naiire , Kahrizsangi Mojgan Baraty , Kafi Fatemeh 
Published: April 2022

Introduction: The spread of COVID-19 rapidly affected the daily lives of individuals, including students. It has led to changes in students' usual curricula and nursing students' lifestyles. It has affected not only on the personal life and health of nursing students but also the lifestyle and health of other people in the community. Therefore, this study aimed to determine the lifestyle of nursing students of Shahid Sadoughi University of Medical Sciences and Health Services during the COVID-19 epidemic in 2020.
Methods: This was a cross sectional study in 2020 on 171 nursing students of Shahid Sadoughi University of Medical Sciences and Health Services using the convenience sampling method. To collect the required data, demographic information form and lifestyle questionnaire during the COVID-19 epidemic were used. The collected data were then analyzed by SPSS software version 16 using descriptive and inferential statistics methods (independent t-test and ANOVA).
Results: The mean and deviation score of students' general lifestyle was at a moderate level, 38.08 ± 16.8. Mean and deviation scores of both happy and healthy lifestyles were at a low level, 21.66 ± 9.4 and 16.42 ± 8.5, respectively. There was no significant relationship between demographic characteristics and mean lifestyle score (P≥0.05).
Conclusion: Due to the fact that students' lifestyle are at a moderate level and the score of lifestyle components (both happy and healthy) was at a poor level, and given that the end of the COVID-19 pandemic is uncertain, therefore, it is necessary for managers and officials of universities and the Ministry of Health to plan timely and effective interventions to improve the lifestyle of nursing students.
KUALITAS AIR TERDAMPAK LIMBAH SEBAGAI INDIKATOR PEMBANGUNAN BERKELANJUTAN DI SUB DAS MARTAPURA KABUPATEN BANJAR
Authors: Sofiana Masdarina , Kadarsah Anang , Sofarini Dini 
Published: April 2022

Banyaknya aktivitas antropogenik seperti pembuangan limbah industri dan domestik ke perairan menyebabkan terjadinya penurunan kulitas air, seperti halnya di sub DAS Martapura Kabupaten Banjar. Tentunya hal ini patut diwaspadai mengingat keberadaan limbah akan berdampak terhadap kualitas lingkungan dan pengembangan kapasitas sumber daya dalam konteks pembangunan berkelanjutan. Tujuan penelitian ini adalah mengetahui kualitas air yang terdampak limbah sehingga dapat dijadikan sebagai indikator perencanaan pembangunan berkelanjutan di sub DAS Martapura secara khusus. Lokasi penelitian berada di sub DAS Martapura Kabupaten Banjar dengan 4 titik sampling berdasarkan keberadaan sumber limbah yang dominan. Kualitas air sungai diukur berdasarkan parameter fisika dan kimia. Analisis kualitas dan penentuan status mutu air menggunakan metode STORET. Hasilnya adalah (1) Parameter fisika-kimia perairan yang terdampak pencemaran di sub DAS Martapura Kabupaten Banjar terutama : DO, BOD, COD, pH dan suhu pada beberapa stasiun pengamatan nilainya telah melebihi baku mutu air sungai Kelas II menurut PP No. 22 Tahun 2021. (2) Hasil perhitungan status mutu air pada sub DAS Martapura menunjukkan total skor sebesar -18. Kondisi perairan di sub DAS Martapura menunjukkan diperlukannya usaha mitigasi dan adaptasi dampak pencemaran lingkungan yang terintegrasi untuk mendukung program pembangunan berkelanjutan. Kata kunci: Kualitas Air, Metode STORET, Sub DAS Martapura. Numerous anthropogenic activities like industrial and domestic waste disposal into the waters causes decreasing water quality, as is the case in the Martapura sub-watershed, Banjar Regency. This should be watched out for considering waste will affect environmental quality and resource capacity development in context of sustainable development.The research purpose was to determine the water quality affected by waste, using it as an indicator of sustainable development planning in the Martapura sub-watershed specifically. The research location was the Martapura sub-watershed with 4 sampling points based on the presence of dominant waste source. River water quality was measured based on physical and chemical parameters. Quality analysis and determination of water quality status was done using the STORET method. The results are (1)The physico-chemical parameters of waters affected by pollution in the Martapura sub-watershed, Banjar Regency, especially: DO, BOD, COD, pH and temperature at several observation stations have exceeded the Class II river water quality standard according to Government Regulation No. 22 of 2021. (2)The results of the calculation of water quality status in the Martapura sub-watershed show a total score of-18. Martapura sub-watershed waters condition showed the need for integrated mitigation and adaptation of environmental pollution impacts to support sustainable development programs. Keywords: Martapura Sub-Watershed, STORET Method, Water Quality.
KAJIAN PERBAIKAN SIFAT FISIKA DAN KIMIA TANAH PASCA TAMBANG MENGGUNAKAN METODE COMPOSTING BERBAHAN DASAR SAMPAH ORGANIK DENGAN VARIASI AKTIVATOR MOL DAN EM4
Authors: Destania Frissilia , Prihatini Nopi Stiyati 
Published: April 2022

Artikel ini bertujuan untuk menganalisis metode composting berbahan dasar sampah organik dengan aktivator MOL dan EM4 dalam memperbaiki sifat fisika dan kimia tanah pasca tambang. Permasalahan utama dari tanah pasca tambang adalah sedikitnya kandungan unsur hara, memiliki pH yang rendah (masam), dan minimnya nilai C-organik pada tanah. Tanah pasca tambang berada di fase kerusakan dan perlu dilakukan pembenahan. Pembenahan dapat dilakukan dengan penambahan kompos pada tanah pasca tambang. Kompos merupakan pupuk organik yang berasal dari proses pelapukan bahan-bahan organik. Aktivator kompos berfungsi untuk mempercepat proses dekomposisi pada kompos. Penggunaan aktivator EM4 dan MOL bertujuan untuk mengetahui perbedaan kualitas kompos yang dihasilkan. Penambahan aktivator MOL dan EM4 dapat mempengaruhi kualitas kompos dan dari kedua aktivator tersebut tidak memberikan perbedaan yang terlalu signifikan sehingga kedua nya efektif digunakan sebagai aktivator kompos. Penambahan kompos pada tanah pasca tambang mampu meningkatkan unsur hara tanah. Hal ini terbukti dari beberapa hasil penelitian yang menunjukan adanya perubahan warna tanah menjadi kehitaman, peningkatan nilai C-organik, pH netral, serta peningkatan nilai N, P dan K pada tanah pasca tambang. Kata kunci: EM4, Komposting, MOL, Tanah. This article aims to analyze the composting method based on organic waste with MOL and EM4 activators to improve the physical and chemical properties of post-mining soil. The main problems of post-mining soil are the lack of nutrients, have a low pH (acidic), and the minimal value of C-organic in the soil. The post-mining soil is in the damage phase and needs to be improved. Improvements can be made by adding compost to the post-mining soil. Compost is an organic fertilizer that comes from the weathering process of organic materials. Compost activator serves to speed up the decomposition process in compost. The use of EM4 and MOL activators aims to determine the difference in the quality of the compost produced. The addition of MOL and EM4 activators can affect the quality of compost and the two activators do not provide too significant differences so that both are effectively used as compost activators. The addition of compost to post-mining soil can increase soil nutrients. This is evident from several research results which show a change in soil color to black, an increase in the value of C-organic, neutral pH, and an increase in the value of N, P and K in post-mining soil. Keywords: Composting, EM4, MOL, Soil.
KOMBINASI AERASI TERDIFUSI, BIOSAND FILTER DAN KARBON AKTIF UNTUK MENGOLAH LIMBAH DOMESTIK
Authors: Nurhayati Indah , Syafi'i Muhammad 
Published: April 2022

Biosand filter adalah salah satu teknologi yang dapat diterapkan untuk mengolah air limbah domestik skala rumah tangga. Penelitian ini bertujuan untuk menganalisis kombinasi aerasi, biosand filter dan adsobsi dalam menurunkan COD dan fosfat, mengkaji pengaruh debit terhadap penurunan COD dan fosfat. Reaktor terdiri dari bak aerasi volume 50 L dengan aerator terdifusi dengan tipe TAKARI AT-350, kolom biosand filter dari pipa PVC 8” tinggi 75 cm dengan media pasir halus, pasir kasar dan kerikil, dan kolom adsorpsi dari pipa PVC 6” tinggi 15 cm dengan adsorben karbon aktif granul. Penelitian dilakukan dalam skala laboratorium dengan sistem batch. Variasi yang digunakan adalah debit aliran 0,9 ml/detik dan 3,6 ml/detik. Reaktor beroperasi selama 7 hari, pengambilan sampel dilakukan setiap hari pada inlet dan outlet. Hasil penelitian menunjukkan bahwa semakin kecil debit semakin tinggi penurunan COD dan fosfat. BSF1 debit 0,9 ml/detik dapat menurunkan COD sebesar 92% dan fosfat 88%; BSF2 debit 3,6 ml/detik dapat menurunkan COD sebesar 75% dan fosfat 78%. Kata Kunci : Aerasi, Air Limbah Domestik, Biosand Filter, Karbon Aktif. The biosand filter is a technology that can be applied to treat domestic wastewater on a household scale. This study aims to analyze the combination of aeration, biosand filter, and adsorption in reducing COD and phosphate, examine the effect of discharge on the reduction of COD and phosphate. The reactor consists of an aeration tank with a volume of 50 L with a diffused aerator of the TAKARI AT-350 type, a biosand filter column made of 8” PVC pipe 75 cm high with fine sand, coarse sand and gravel media, and an adsorption column made of 6” PVC pipe 15 cm high, with granular activated carbon adsorben. Research on a laboratory scale with a batch system. Variations used are flow rates of 0.9 ml/second and 3.6 ml/second. The reactor operates for 7 days, sampling is done every day at the inlet and outlet. The results showed that the smaller the discharge, the higher the decrease in COD and phosphate. BSF1 discharge of 0.9 ml/second can reduce COD by 92% and phosphate by 88%; BSF2 of 3.6 ml/second can reduce COD by 75% and phosphate by 78%. Keywords: Activated Carbon, Aeration, Biosand Filter, Domestic Wastewater.
Garut (Marantha Arundinaceae L.) Starch-Based Cookies with Rice Bran and Whole Wheat Flour as a Source of Fiber
Authors: Kifayah Rofi’ul , Basori Basori 
Published: April 2022

The escalation of degenerative diseases can be prevented by consuming fibrous foods. This study focus onproduct development of cookies from arrowroot starch with the addition of rice bran and whole wheat flour as fiber source. The addition of fiber was conductedusing various concentration of 0-30%. Aim of this study was determinedto add therice bran and whole wheat flour against physical-chemical properties of cookies. The data of physical-chemical properties were analyzed by Anova, followed by HSD test, while the organoleptic data were analyzed using Friedman test and determining the best treatment using method effectiveness index (De Garmo). Best quality wasshowedon proportion of20% rice bran flour (P2) and 20% whole wheat (P5) with the average value of the water content in sequence P2 and P5 are: 2.50% and 2.67%, 1.44% and dietary fiber 1.67%, 8.35% protein and 57.12%, and 60.73% texture and 65.80%. Organoleptic datashowedpreferable panelist on the color scale of 3.69 (regular) and 4.8 (a bit like), a sense of scale 4.13 (regular) and 4.77 (a bit like), aroma scale of 4.57 (slightly like) and 4.73 (a bit like), and the texture scale 4.37 (regular) and 4.33 (regular).
Annual and seasonal trends in Actual Evaporation over different meteorological sub-divisions in India using GLEAM data
Authors: Kanike Raghavendra Prasad , Bhimala Kantha Rao , Patra Gopal Krishna , S Himesh 
Published: April 2022

Abstract
The present study analyzed the actual evaporation (Ea) and its components [transpiration (Et), bare soil evaporation (Eb), interception loss (Ei), and open water evaporation (Eo)] data to study the long-term (1980-2018) trends over different meteorological sub-divisions in India. Quantitatively, all India annual Ea is 573 mm (σ= 28.8 mm), where Et (µ=455.9 mm; σ= 30.4 mm) plays a major role compared to other evaporation processes like Eb (µ=55.9 mm; σ= 8.6 mm), Ei (µ=33.5mm; σ= 3.4 mm), and Eo (µ=27.2 mm; σ=0.38 mm). The MK test reveals an increasing trend (1.33mm/y) in annual Ea due to the rising trend in Et (1.91mm/y) and Ei (0.16mm/y) even though there is no significant trend in rainfall or potential evaporation (Ep). The sub-division-wise analysis shows the increasing trend in Ea observed over irrigated regions located in the south, north-west, and foothills of the Himalayas during pre-monsoon and monsoon season. The correlation analysis observed a complex relationship between Ea and climatic factors (rainfall (RF), soil moisture (SM), surface temperature (T), relative humidity (RH), surface shortwave radiation (SSR), and wind speed (WS)) during monsoon season such that the water-limited areas have a positive correlation with SM, RH and RF, and negative correlation with WS, T, and SSR, whereas, in energy-limited areas (east India), the Ea showed a positive correlation with SSR & T and negative correlation with RF. The main climatic drivers for the increasing trend of Ea are SM & rainfall over dry regions and SSR &T over densely vegetated regions in India.
Reversible 2’-OH Acylation Enhances RNA Stability
Authors: Kool Eric , Fang Linglan , Xiao Lu , Jun Yong Woong , Onishi Yoshiyuki 
Published: April 2022

Abstract
Stabilization of RNAs for storage, transport, and biological application remains a serious challenge, particularly for larger RNAs that are not accessible by chemical synthesis. Here, we present reversible 2’-OH acylation as a general strategy to preserve RNA of any length or origin. High-yield polyacylation of 2’-hydroxyls (“cloaking”) by readily accessible acylimidazole reagents effectively shields RNAs from both thermal and enzymatic degradation. Subsequent treatment with water-soluble nucleophilic reagents removes acylation adducts quantitatively (“uncloaking”) and recovers a remarkably broad range of RNA functions, including reverse transcription, translation, and gene editing. Furthermore, we show that certain α-dimethylamino- and α-alkoxy- acyl adducts are spontaneously removed in human cells, restoring mRNA translation with extended functional half-lives. These findings support the potential of reversible 2’-acylation as a simple and general molecular solution for enhancing RNA stability, and provide mechanistic insights for stabilizing RNA regardless of length or origin.
Neuro-Psychiatric Symptoms in Civilian Survivors of Urban Missile Attacks
Authors: Ashkenazy Rotem Saar , Naparstek Sharon , Dizitzer Yotam , Zimhoni Noa , Friedman Alon , Shelef Ilan , Cohen Hagit , Shalev Hadar , Oxman Liat , Novack Victor , Ifergane Gal 
Published: April 2022

Abstract
Background: Blast-explosion may cause traumatic brain injury (TBI), leading to post-concussion syndrome (PCS). In studies on military personnel, PCS symptoms are highly similar to those occurring in post-traumatic-stress-disorder (PTSD), questioning the overlap between these syndromes. In the current study we assessed PCS and PTSD in civilians following exposure to rocket attacks. We hypothesized that PCS symptomatology and brain connectivity will be associated with the objective physical exposure, while PTSD symptomatology will be associated with the subjective mental experience. Methods: 289 residents of explosion-sites have participated in the current study. Participants completed self-report of PCS and PTSD. The association between objective and subjective factors of blast and clinical outcomes was assessed using multivariate analysis. White-matter (WM) alterations and cognitive abilities were assessed in a sub-group of participants (n=46) and non-exposed controls (n=16). Non-parametric analysis was used to compare connectivity and cognition between the groups. Results: Blast-exposed individuals reported higher PTSD and PCS symptomatology. Among exposed individuals, those who were directly exposed to blast, reported higher levels of subjective feeling of danger and presented WM hypoconnectivity. Cognitive abilities did not differ between groups. Several risk-factors for the development of PCS and PTSD were identified. Conclusions: Civilians exposed to blast present higher PCS/PTSD symptomatology as well as WM hypoconnectivity. Although symptoms are sub-clinical, they might lead to the future development of a full-blown syndrome and should be considered carefully. The similarities between PCS and PTSD suggest these are not distinct syndromes, but rather represent different ends on a spectrum of a combined disorder.
A novel six-methylated pseudogenes signature predicts prognosis of glioma patients
Authors: Chen Zhigang , Zhou Jialin , Wang Bingran , Li Jiahui , Xie Han , Zhao JiaJia , Liu Jun , Tian Dasheng , Bian Erbao 
Published: April 2022

Abstract
Background: Gliomas are the most common malignant tumor from the central nervous system(CNS). Dysregulated pseudogene expression was significantly associated with the prognosis of glioma patients. However, the role of abnormal methylation of pseudogenes in glioma prognosis has not yet been studied. This study aimed to develop a novel six-methylated pseudogenes signature to predict prognosis of glioma patients.Methods: Based on multiple screening, a risk signature for six-methylated pseudogenes was constructed, and then classified glioma patients into high-risk and low-risk groups. Next, a prognostic nomogram including grade, age, gender, and radiation was constructed. Besides, the immune cell infiltration analyses of patients based on the six-methylated pseudogenes in two risk groups were performed. Meanwhile, consensus cluster analysis of six methylated pseudogenes identified two glioma patient subgroups (cluster1/2). Furthermore, Gene Ontology (GO) , Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA) were used to analyzed related genes. Finally, the ability of glioma to proliferate, migrate and invade were used to verify subsequent functions. Results: In this study, six gene models consisting of methylated pseudogenes were identified and validated, and showed strong prognostic power in the training dataset, validation dataset, and entire dataset. The calibration diagram showed good predictive performance. In addition, the proportion of B cells and CD4+T cells was significantly higher in the high-risk group, while the proportion of Mono cells was lower. By silencing the expression of SBF1P1 and SUMO1P1, the ability of glioma proliferation, migration and invasion can be inhibited.Conclusion: The six-methylated pseudogenes signature may be a novel predictor for prognostic assessment of glioma patients, which could accurately predict patient prognosis.
Molecular characteristics of Yersinia enterocolitica and related species in Ningxia, China based on whole-genome sequencing
Authors: Yue Yuan , Shen Mei , Liu Xiang , Hao Qiong , Xu Shuai , Liu Zhiguo , Hou Xuexin , Jing Huaiqi , Zhou Xuezhang , Li Zhenjun 
Published: April 2022

Abstract
Background: Yersinia enterocolitica is a common zoonotic pathogen and has emerged as an important foodborne pathogen worldwide. Y. enterocolitica has been sporadically recovered from animals, foods, and human clinical samples in various regions of Ningxia. Molecular characteristics of Y. enterocolitica in the Ningxia Hui Autonomous Region was not well described in current studies.Methods: The molecular characteristics of 271 Yersinia strains isolated from the Ningxia Hui Autonomous Region were analyzed using SNP and core gene multilocus sequence typing (cgMLST). The isolates were taken from animals (n = 208), food (n = 50), and patients (n = 13) between 2007 and 2019. Results: The SNP results indicated that the 271 isolates were divided into 12 species, Y. enterocolitica (n=187), Y. intermedia (n=31), Y. massiliensis (n=30), Y. mollaretii (n=7), Y. pekkanenii (n=5), Y. proxima (n=4), Y. alsatica (n=2), Y. frederiksenii (n=1), Y. kristensenii (n=1), Y. hibernica (n=1), Y. canariae (n=1), and Y. rochesterensis (n=1). Of the 187 Y. enterocolitica isolates, 81.28% (n = 152) were of animal origin, the food source was 12.30% (n = 23) and the patient source was 6.42% (n = 12). The clustering of Y. enterocolitica strains were closely related to the biotype. In total, 187 Y. enterocolitica strains were classified into 54 sequence types (STs) and 125 cgMLST types (CTs). The most common STs were ST429, which were significantly associated with serotype O:3. 4/O:3 was the dominant bioserotype in the Ningxia region, which differed from most of China. The 125 CTs clustered to form 55 microclades of the HC100 (hierarchical clustering). Conclusion: The results indicated that the distribution of Y. enterocolitica in the Ningxia Hui Autonomous Region was both general and specific and differed to some extent from the distribution elsewhere in China and abroad. The difference in the distribution of virulence genes between pathogenic and non-pathogenic isolates was attributed to the presence or absence of the type III secretion system. Swine farming and meat food cause human infection and transmission.
An atlas of human dental pulp cells in multiple spatial and temporal levels based on single-cell sequencing analysis
Authors: Ren Huihui , Wen Quan , Zhao Qingxuan , Wang Nan , Zhao Yuming 
Published: April 2022

Abstract
Background The dental pulp plays a crucial role in the long-term maintenance of teeth function. The progress of endodontic treatment and pulp tissue regeneration engineering has therapeutic potential. But regulation mechanisms of tooth development or regeneration dependent on dental stem cells were still not fully understood. So, it is urgently needed bridge the gaps between basic and clinical research. With single cell sequencing technology being applied in medical research, landscapes of human dental pulp cells had been initially outlined. However, the specific cellular heterogeneity of dental pulp cells, especially dental stem cells in different spatial and temporal levels are remain unclear. Methods Gene expression profiles of human dental pulp cells from four teeth with different developmental periods analyzed by 10x Genomics single-cell RNA sequencing were studied and cluster analysis. Multiple spatial and temporal characteristics of subpopulation of cells were further studied by signal pathway analysis, trajectory analysis stemness analysis. Results In this study, transcriptomic expression-based clustering analysis showed that dental pulp cells at different developmental points exist differences in number, not cell types. Pathway analysis revealed that early in development, the pulp may perform more developmentally relevant functions such as synthetic translation, while later it begins to differentiate into specific directions or retains functional characteristics for homeostasis maintenance. The importance of the stem cell microenvironment in human tooth development was evident. Subpopulations in early development stage were clearly identified in fibroblasts, odontoblasts and mesenchymal stem cells. Particularly, differentially expressed gene analysis and stemness analysis defined three subpopulations of dental pulp stem cells with greater stemness and potential for multiple differentiation. Combining the expression characteristics of the three cell subpopulations, some genes such as MIA, DGKI and so on that may contribute to the developmental differentiation of dental pulp stem cells were also uncovered Conclusion For the first time, the specific cellular heterogeneity of dental pulp cells, especially dental stem cells in different spatial and temporal level was proviede. Gene expression profile of early developing cells may help in cell screening for regenerative engineering and improve the success of dental pulp regeneration.