bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mitochondrial genome of Binodoxys acalephae (Hymenoptera: Braconidae) with unique gene rearrangement and phylogenetic implications
Authors: Xu Shiwen , Li Weiwei , Liu Qiannan , Wang Yunming , Li Xiaoling , Duan Xiaoqian , He Jia , Song Fan 
Published: November 2022

Abstract
Background: Species in the subfamily Aphidiinae from the Braconidae of Hymenoptera are endoparasitic wasps that exclusively utilize aphids as hosts. Some Aphidiinae species are widely used as biological agents. However, there were only one species with determined complete mitochondrial genome from this subfamily.
Methods and Results: In this study, we sequenced and annotated the mitochondrial genome of Binodoxys acalephae, which was 15,116 bp in size and contained 37 genes. The start codon of 13 protein-coding genes was ATN, and the complete stop codon TAA and TAG was widely assigned to 11 protein-coding genes. The lrRNA contains 43 stem-loop structures, and srRNA contains 25 stem-loop structures. Remote inversion of tRNA genes was found to be dominant in B. acalephae. Compared with the ancestral organization, two tRNA genes (tRNACys and tRNATyr) were translocated and three tRNA genes (tRNAIle, tRNAMet and tRNALeu(CUN)) were remote inverted. Within Braconidae, gene clusters tRNATrp-tRNACys-tRNATyrand CR-tRNAIle-tRNAGln-tRNAMet were hotspots for gene rearrangement. Phylogenetic analysis showed that both Bayesian and maximum-likelihood methods recovered the monophyly of Aphidiinae and suggested that Aphidiinae was the earliest branching lineage of Cyclostomes, forming sister clades with the remaining subfamilies. The phylogenetic analyses of nine subfamilies supported the monophyly of Cyclostomes and Noncyclostomes in Braconidae.
Conclusion: The arrangement of mitochondrial genes and the phylogenetic relationships among nine Braconidae subfamilies were constructed better to understand the diversity and evolution of Aphidiinae mitogenomes.
Deep Ensemble Transfer Learning-based Approach for Classifying Hot-Rolled Steel Strips Surface Defects
Authors: Bouguettaya Abdelmalek , Mentouri Zoheir , Zarzour Hafed 
Published: November 2022

Abstract
Over the last few years, advanced deep learning-based computer vision algorithms are revolutionizing the manufacturing field. Thus, several industry-related hard problems can be solved by training these algorithms, including flaw detection in various materials. Therefore, identifying steel surface defects is considered one of the most important tasks in the steel industry. In this paper, we propose a deep learning-based model to classify six of the most common steel strip surface defects using the NEU-CLS dataset. We investigate the effectiveness of two state-of-the-art CNN architectures (MobileNet-V2 and Xception) combined with the transfer learning approach. The proposed approach uses an ensemble of two pre-trained state-of-the-art Convolutional Neural Networks, which are MobileNet-V2 and Xception. To perform a comparative analysis of the proposed architectures, several evaluation metrics are adopted, including loss, accuracy, precision, recall, F1-score, and execution time. The experimental results show that the proposed deep ensemble learning approach provides higher performance achieving an accuracy of 99.72% compared to MobileNet-V2 (98.61%) and Xception (99.17%) while preserving fast execution time and small models’ size.
Validation of the Methods for the Non-milk Fat Detection in Artificially Adulterated Milk With Palm Oil
Authors: El-Nabawy Marwa , Awad Sameh , Ibrahim Amel 
Published: November 2022

Abstract
Background
Dairy products are among the most adulterated food products. Especially the milk fat can be replaced with foreign fats or oils. Because of the current high price of milk fat, it has been replaced by low-cost oils, especially those oils that have the same fatty acid profile as milk fat. This study aims to validate some classic methods (saponification number and iodine number) and the use of modern devices (gas chromatography, reverse phase high performance liquid chromatography and Fourier Transform Infrared) to detect palm oil in milk and to confirm the minimum levels of palm oil added to milk. Five treatments of fresh milk cream and an emulsion of palm oil were used to prepare different levels of palm oil in the final liquid milk.
Results
The results of this study showed that the values of the saponification number decreased with the increase in the percentages of added palm oil, but there was no decrease from the limits of the Egyptian standards until the addition of 25% palm oil. The iodine number is less sensitive than the saponification number in the detection of palm oil. Butyro refractometer reading is unable to detect the palm oil in milk. The fatty acid profile in milk determined by gas chromatography correlated well with the addition of palm oil. Also, there is a good relationship between the cholesterol and β-sitosterol content measured by reverse phase high performance liquid chromatography and the level of added palm oil. There was no relationship between the behavior of the spectra resulting from Fourier transform infrared spectroscopy and the presence of palm oil.
Conclusions
Gas Chromatography has potential to be used for the detection of palm oil in milk, the levels of some fatty acids in the milk could be used as an indicator. Also, Reverse Phase High Performance Liquid Chromatography analysis could be used for the same purpose, as the addition of palm oil caused a gradual decrease in the cholesterol percentage and increase in the β-sitosterol content of milk.
Psychotic experiences in Afghanistan as a reaction to a highly stressful environment
Authors: Kovess-Masfety Vivianne , Sabawoon Ajmal , Keyes Kerry , Karam Elie 
Published: November 2022

Abstract
Objectives
To estimate the prevalence and demographic, psychiatric, and trauma-focused correlates of psychotic experiences (PEs) in the Afghan general population
Methods
Data were drawn from a cross sectional household survey implemented in eight regions of Afghanistan (N = 4445). The CIDI structured instrument was administered to adults to assess psychiatric disorders and psychotic experiences; life events and PTSD were assessed validated instruments. Weighted multivariate models integrated socio-demographics, regions, traumas as determinants of PE.
Results
PEs were frequently reported in the Afghan population: 27.50% of the population reported a lifetime PE. PEs were more common among specific ethnic groups, and were associated with lower income in adjusted regression models. PEs were associated with mental health problems including major depressive disorders (OR = 3.43), PTSD (OR = 5.08), generalized anxiety (OR = 4.2); life-time suicidal attempts (OR 6.04), lifetime suicidal thoughts (OR = 3.42), addiction (OR = 2.18); and psychological distress and impairment due to mental health (OR = 2.95 and 2.46, respectively).
Conclusion
Psychotic experiences in the Afghan general population confirm general population findings in other countries, that psychotic experiences are common and associated with economic and social marginalization, and part of a continuum of mental health problems experienced in populations. Efforts to reduce and treat psychotic experiences within a broad array of psychiatric conditions are needed.
Comprehensive analysis based on methylation microarrays and single-cell transcriptomics in esophageal squamous cell carcinoma
Authors: Xiao Qiyu , Huang Qingguan 
Published: November 2022

Abstract
Aberrant methylated genes (DMGs) play an important role in the etiology and pathogenesis of esophageal squamous cell carcinoma (ESCC). Single-cell transcriptome analysis of immune cells in tumors provides a way to comprehensively study these cells in a highly complex tumor microenvironment (TME). In this study, we combine methylation datasets and single cell datasets to ascertain aberrant methylated-differentially expressed genes and pathways associated with ESCC TME by comprehensive bioinformatics analysis. STRING and Cytoscape software were used to construct a protein-protein interaction (PPI) network. In total, 111 hypomethylated/high-expressed genes were identified at the screening step, and finally found six mostly changed hub genes including COL1A1, COL1A2, COL5A1, COL5A2, COL11A1 and THBS2. Pathway analysis indicated that aberrantly methylated DEGs mainly associated with extracellular matrix structural constituent and cytokine − cytokine receptor interaction. Analyzing the single cell data of ESCC, we confirmed the specific cell cluster highly expressing hub genes and predict the biological function of the cell cluster. These results can shed a light for screening and diagnosis of ESCC in future.
A study on internet use and well-being among Chinese older adults: based on CGSS (2012-2018) five-wave mixed interface survey data
Authors: Zhang Chong , Wang Yan 
Published: November 2022

Abstract
This study based on the five-wave mixed interface survey data of China General Social Survey (CGSS) in 2012, 2013, 2015, 2017, and 2018, we selected a total of 18,458 older adults aged 60 and above, comprehensively used ordered probit regression, propensity score matching (PSM), and Karlson–Holm–Breen (KHB) to investigate the relationship between Internet use and well-being of the older adults in China, and to analyze the mediating role of subjective social fairness in the above relationship. The analysis results show that Internet use contributes to the improvement of well-being in older adults, but attenuates this effect by reducing the sense of social fairness. In addition, place of residence, education, and regional factors moderated the effect of Internet use on well-being. The well-being effect of Internet use is significant for urban older adults, as does schooled or eastern-central older adults. The above results indicate that moderate use of the Internet is of great significance in promoting active aging, but further attention should be paid to the aging transformation of the Internet in the process of promoting the digital integration of the elderly.
Assessing the Impact of Organic and Inorganic Pollutants Released from a Wastewater Treatment Plant on Humans and Aquatic Environment, Al-Hoceima City, Morocco
Authors: HAMMOUDANI Yahya EL , DIMANE Fouad , HABOUBI Khadija , BOURJILA Abdelhak , BENAISSA Chaimae , ACHOUKHI Iliass , HABOUBI Chaimae 
Published: November 2022

Abstract
Wastewater contains a variety of compounds qualified as pollutants. These undergo incomplete treatment in wastewater treatment plants. The objective of this study is to determine the potential impacts on humans and aquatic environment of 46 organic and inorganic micropollutants using the USE-tox® model. The concentrations used in this study are obtained by analyzing raw and treated wastewater from the wastewater treatment plant of the city of Al-Hoceima, Morocco. The total human health impact score is 10 − 2, generally varying between 10 − 3 and 10 − 9. Ba, Hg, Zn and Cd had the highest score with a percentage of 92% of the total score. For the aquatic environment, impact was estimated for 25 compounds. Pyrene, Anthracene, Benzo(a)Anthracene, Fluoranthene and PCB-77 were the major contributors with an impact ranging from 3.43E + 02 to 1.21E + 01 PDF.m 3 .d. With a value of 3.43E + 02, Pyrene had the highest impact, contributing 73% by itself.
Negative Effect of High-Calorie High-Salt Diet on Spleen Oxidant/Antioxidants and Structure: Preventive Effects of Sprint Interval Training
Authors: Aghlmand Saeideh , Nasiri Esmaeil , Rahmani Mohammad , Hesari Ali Kalantari 
Published: November 2022

Abstract
We investigated the effect of a high-calorie high-salt diet and sprint interval training on oxidant/antioxidant status and structural changes in the spleen of male Wistar rats. Eighteen male Wistar rats were randomized into three groups: normal diet (ND), high-calorie high-salt diet (HCSD), and HCSD + sprint interval training (HCSD + SIT). Rats in HCSD and HCSD + SIT groups were under a high-calorie high-salt diet. The SIT (4–9 reps of 10s duration sprints) performed 3 sessions/week for 8 weeks. Forty-eight hours after the last training session, the spleen was removed and used for the assessments of oxidant/antioxidant status, and histomorphometric parameters. Results revealed that compared to the ND group, the super oxide dismutase (SOD) activity, the glutathione peroxidase (GPx) level, and the total antioxidant capacity (TAC) were significantly lower (P < 0.001), and the malondialdehyde (MDA) levels were significantly higher in the spleen tissue of the HCSD group (P < 0.001), but there was no significant difference between the HCSD + SIT and ND groups in none of the assessed variables (P > 0.05), Moreover, there was a significant increase in the red to white pulp ratio and a significant decrease in the number of lymph cells and splenic trabecular volume in the HCSD group (P < 0.001). Nonetheless, in none of the assessed morphometric parameters, a significant difference was observed between the HCSD + SIT and ND groups (P > 0.05). It seems that SIT training may avert HCSD-induced unfavourable changes in the spleen oxidant/antioxidant status and counteract the deleterious effects of the HCSD on the spleen structure.
Evaluation of Diagnostic Efficacy of NRP-1/CD304 in Hematological Diseases
Authors: Liu Yi-jun , Li Xiao-hui , Song Yi-ling , Zhou Yi-chen , Cai Rong-zeng , Chi Pei-dong 
Published: November 2022

Abstract
BACKGROUND: Previous studies had explored the diagnostic or prognostic value of NRP-1/CD304 in blastic plasmacytoid dendritic cell neoplasm (BPDCN), acute myeloid leukemia (AML) and B-cell acute lymphoblastic leukemia (B-ALL), whereas the expression and application value of NRP-1/CD304 in other common hematological diseases have not been reported.
METHODS: Bone marrow samples from 297 newly diagnosed patients with various hematological diseases were collected to detect the expression of NRP-1/CD304 by flow cytometry (FCM). The diagnostic efficacy of NRP-1/ CD304-positive diseases was analyzed by receiver operating characteristic (ROC) curve, and the area under the ROC curve (AUC) was compared.
RESULTS: In the research cohort, the total positive rate of NRP-1/CD304 was 14.81% (44/297), mainly distributed in BPDCN (100%, 6/6), B-ALL (48.61%, 35/72) and AML (4.48%, 3/67), with statistically significant differences (P<0.01). Other diseases, such as T-cell acute lymphoblastic leukemia (T-ALL), B-cell non-Hodgkin lymphoma (B-NHL), T/NK-cell lymphoma and plasma cell neoplasms, did not express NRP-1/CD304. The sensitivities of NRP-1/CD304 for BPDCN, B-ALL and AML were 100.00%, 48.61% and 4.48%, as well as the specificities of which were 87.12%, 96.01% and 82.48%, respectively. The AUC of NRP-1/CD304 was 0.936 (95%CI 0.898 ~ 0.973), 0.723 (95%CI 0.646 ~ 0.801) and 0.435 (95%CI 0.435) in BPDCN, B-ALL and AML respectively.
CONCLUSIONS: NRP-1/CD304 is only expressed in BPDCN, B-ALL and AML, but not in other common hematological diseases. This indicates that NRP-1/CD304 has no obvious diagnostic and follow-up study value in hematological diseases other than BPDCN, B-ALL and AML.
The impact of earlywood and latewood on the compressive strength of Douglas fir
Authors: Li Wanzhao , Yang Kai , Wang Junfeng , Wang Xinzhou , Mei Changtong , Bulcke Jan Van den , Acker Joris Van 
Published: November 2022

AbstractMechanical performance of wood is determined by its structural characteristics, among others earlywood and latewood in softwoods. Given that thermal modification also affects the mechanical strength, it is key to understand how the modification affects earlywood and latewood and how this on its turn impacts compressive strength. In this study, Douglas fir blocks measuring 30×50×150mm3were modified at 180°C (TM-180°C) and 210°C (TM-210°C). The compressive strength of pure earlywood (EW), pure latewood (LW) and a combination of earlywood and latewood (ELW) specimens was measured. The specimens were compressed in terms of 30% of their original thickness, and during the compression test the strain distribution of ELW was recorded. In addition, the microstructure before and after compression was investigated complemented with using SEM to understand the structural changes taking place. The results show that compressive strength of TM-180°C specimens was high, most probably because thermal modification increased stiffness of cell walls and homogenized strain distribution in ELW specimens. Control specimens had a higher compression set recovery than thermally modified specimens. Tracheid cell walls in EW and LW specimens were flattened and buckled respectively due to compression. While in thermally modified material, cell wall fissures and wood ray fractures in EW and LW specimens respectively were observed. For ELW specimens, structural changes in latewood were trivial, and structural changes in the earlywood part were less significant than in full EW specimens. Compared to EW specimens, earlywood in ELW showed higher compression set recovery. It seems that structural failure in earlywood is limited when in combination with latewood, resulting from homogenized strain distribution in earlywood.
The mediating effect of depressive symptoms on the association between childhood friendship and physical function in middle-aged and older adults: Evidence from the China Health and Retirement Longitudinal Study (CHARLS)
Authors: Zeng Qingping , Ding Jiali , Tu Raoping , He Huihui , Wang Suhang , Huang Yujia , Wang Zhiyao , Chen Qi , Lu Guangyu , Li Yuping 
Published: November 2022

Abstract

Purpose
This study examines the extent to which depressive symptoms mediate the link between childhood friendship (CF) and physical function among middle-aged and older adults in China.
Methods
The data originated from the China Health and Retirement Longitudinal Study. The Sobel test and multivariable logistic regression were performed to examine the mediating role of depressive symptoms (measured by the 10-item Center for Epidemiologic Studies Depression Scale) in the association between CF (measured by a standardized retrospective questionnaire) and physical function, which was measured by basic activities of daily living (BADL) disability, instrumental activities of daily living (IADL) disability, and grip strength.
Results
A total of 12,170 participants aged 45 or older were included in this cross-sectional study. After controlling for covariates, worse CF was associated with an increased prevalence of BADL disability (OR = 1.18; 95% CI = 1.05–1.32), IADL disability (OR = 1.25; 95% CI = 1.12–1.40), and low grip strength (OR = 1.21; 95% CI = 1.09–1.34). The proportion of the mediating effect of depressive symptoms was 49% in CF and BADL, 41% in CF and IADL, and 12% in CF and grip strength. Depressive symptoms and worse CF have a joint effect on BADL disability (OR = 3.30; 95% CI = 2.82–3.85), IADL disability (OR = 3.52; 95% CI = 3.03–4.09), and low grip strength (OR = 1.65; 95% CI = 1.43–1.92).
Conclusions
Adults with better CF were more likely to have a decreased prevalence of impaired physical function in their later lives. Depressive symptoms acted as a mediator associated with the development of CF. Interventions focusing on the improvement of CF and depression may help improve physical function in adults.
A Pan-Cancer Analysis of the Oncogenic Role of YKT6 in Human Tumors
Authors: Zhang Xuezhong , Dapar Mark Lloyd G. , Chen Yingjun , Liu Tonggang 
Published: November 2022

Abstract
YKT6, as a Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein with vesicle trafficking, plays an essential role in the development and progression of tumor. However, the gene of YKT6 has not been fully assessed in pan-cancer studies. We aim to investigate the gene of YKT6 across 33 different types of tumor by using the Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) database, and other several kinds of bioinformatic tools. YKT6 is significantly up-regulated in most tumors, and we found that overexpression of YKT6 is positively associated with poor prognosis of overall survival (OS) and poor disease-free survival (DFS) prognosis in several tumors, such as Adrenocortical carcinoma (ACC), Bladder Urothelial Carcinoma (BLCA), Head and Neck squamous cell carcinoma (HNSC). We also detected distinct associations exist between YKT6 and tumor mutational burden (TMB) or microsatellite instability (MSI) with tumors. YKT6 expression was positively related to cancer-associated fibroblasts for TCGA tumors of COAD and LGG. Furthermore, we discovered a significantly positively correlation between YKT6 expression and endothelial cell in tumors of COAD, HNSC-HPV+, OV, READ and THCA. While a negative relationship was obtained between YKT6 expression and endothelial cell in KIRC. Moreover, “Syntaxin binding”, “SNARE complex”, “vesicle fusion” and “DNA replication” are involved in the influence of YKT6 on tumor pathogenesis. Our pan-cancer analysis offers a deep comprehending the gene of YKT6 in tumoeigenesis from viewpoint of clinical tumor samples.
Squalene monooxygenase（SQLE) protects ovarian cancer cells from ferroptosis
Authors: Zhang Rong , Zhang Lingmei , Fan Sizhe , Wang Beibei , Wang Lihua , He Zelai , Wang Liangliang 
Published: November 2022

Abstract
Being a novel form of programmed cell death, ferroptosis is different from apoptosis, and significantly inhibits the growth of ovarian cancer cells. In this study, the role of squalene monooxygenase (SQLE) in ferroptosis was investigated due importance of lipid metabolism in ferroptosis and its key involvement in OC. SQLE is the major enzyme responsible for catalyzing cholesterol lipids synthesis and is notably expressed in OCtissues and cells. Reduction of SQLE increase ROS and lipid peroxide accumulation resulting in ferroptosis. Conversely, overexpression of SQLE protects cells from ferroptosis, indicating that SQLE inactivation might be a novel approach as an anti-tumor therapy. The results of thisresearch supported this hypothesis as SQLE inhibition markedly enhanced the antitumor effects of ferroptosis stimulators in OC cells.
All-ferroelectric implementation of reservoir computing
Authors: Chen Zhiwei , Li Wenjie , Fan Zhen , Dong Shuai , Chen Yihong , Qin minghui , Zeng Min , Lu Xubing , Zhou Guofu , Gao Xingsen , Liu Jun-Ming 
Published: November 2022

Abstract
Reservoir computing (RC) offers efficient temporal information processing with low training cost. All-ferroelectric implementation of RC is appealing because it can fully exploit the merits of ferroelectric memristors (e.g., good controllability); however, this has been undemonstrated due to the challenge of developing ferroelectric memristors with distinctly different switching characteristics specific to the reservoir and readout network. Here, we experimentally demonstrate an all-ferroelectric RC system whose reservoir and readout network are implemented with volatile and nonvolatile ferroelectric diodes (FDs), respectively. The volatile and nonvolatile FDs are derived from the same Pt/BiFeO3/SrRuO3 structure via the manipulation of an imprint field (Eimp). It is shown that the volatile FD with Eimp exhibits short-term memory and nonlinearity while the nonvolatile FD with negligible Eimp displays long-term potentiation/depression, fulfilling the functional requirements of the reservoir and readout network, respectively. Hence, the all-ferroelectric RC system is competent for handling various temporal tasks. In particular, it achieves an ultralow normalized root mean square error of 0.017 in the Hénon map time-series prediction. Besides, both the volatile and nonvolatile FDs demonstrate long-term stability in ambient air, high endurance, and low power consumption, promising the all-ferroelectric RC system as a reliable and low-power neuromorphic hardware for temporal information processing.