bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fine Structure of the Defense System of Synanceia verrucosa from, the Red Sea Egypt
Authors: Abd-Elrheem Ali 
Published: August 2022

Abstract
The stonefish has an advanced poisonous gland that has a lumen and medulla consisting of connective tissue types. All authors have studied the poisonous gland of stonefish and they give attention to two types of cells only careless transmission semithin. In the present study, we give attention to the whole structures of this gland by an accurate examination of Electron Micrographs in all gland areas. Also, some authors give attention to secretions only and do not give any attention to the complete structure of the poisonous defense system of an animal. In the present study, the sections were taken in different areas as the front gland, and mid, surround the spine for covering all areas to discover any new structure. This study revealed that the stonefish poisonous gland Synanceia verrucosa in the Red Sea has four types of cells called T.A, T.B, T.C, and T.D appear as (first evidence) in semithin only.
The Correlation between Blepharospasm and Psychological diseases: The Anxiety, Depression and Sleep disorder Study
Authors: Zhang Huina , Gao Qi , Xie Jiajun , Ye Juan 
Published: August 2022

Abstract
Background
To investigate the relationship between Blepharospasm (BEB) symptoms and depression/anxiety/sleep disorder in a prospective manner and to determine whether treatment the BEB with BoNT/A can impact psychological symptoms.
Methods
This prospective interventional case series recruited 61 adults with evidence of BEB. Patients were administered the Jankovic Rating Scale (JRS), the Blepharospasm Disability Index (BSDI), Personal Health Questionnaire Depression Scale (PHQ-8), Generalized Anxiety Disorder 7-item scale (GAD-7) and the Athens insomnia scale (AIS) to evaluate the severity of BEB symptoms, depression, anxiety and sleep disorder before and 1 week, 1 month, 3months after the BoNTA treatment. Statistical analysis was performed to assess the relationships between changes in the survey scores.
Results
The mean score for JRS, BSDI, PHQ-8, and GAD-7 improved significantly (P < 0.0001), respectively, compared to the initial visit at follow-up. At baseline, worse BSDI scores were correlated with worse GAD-7 and PHQ-8, but not with worse AIS. At 1 month follow-up visit, there is no correlation between change in BSDI and PHQ-8/AIS, the change in GAD-7 show a mild association with change in BSDI. The change in BSDI was correlated with the change in both PHQ-8 and GAD-7 in the subgroup of patients without a prior diagnosis of depression or anxiety. Patient satisfaction with BoNT/A treatment reached the highest at one month of follow-up (83.6%, 51/61).
Conclusions
BEB may lead to psychological diseases, BoNT/A can significantly improve motor and non-motor symptoms of BEB, which emphasize the effectiveness of BoNT/A and therefore pave the way for its use in the field of psychiatry.
Trial registration:
Clinicaltrials, NCT04939909. 2021/06/25, https://clinicaltrials.gov/ct2/show/NCT04939909
Mediating effects of suicidal ideation in the relationship between psychological distress and non-suicidal self-injury among adolescents with major depressive disorder: The roles of sex and urban-rural status
Authors: Li Ruifen , Wang Yanni , Huo Lijuan , Lu Shiqi , Hao Jiayue , Li Peng , Zhu Feng , He Kongliang , Zheng Tiansheng , Su Li , Zhou Yongjie 
Published: August 2022

Abstract
Background: Non-suicidal self-injury(NSSI) is a major public concern, and psychological distress(PD) has been found to be an important risk factor of NSSI. However, little is known about the mediating mechanism underlying this relationship. This study aimed at testing the mediating effects of suicidal ideation(SI) in the relationship between PD and NSSI among adolescents with major depressive disorder. Additionally, it's unclear if this effect exists or differs between sexes or urban-rural status.Methods: A total of 428 Chinese adolescents with major depressive disorder(MDD) from 9 provinces across China completed self-reported questionnaires regarding PD, SI and NSSI.Results: The SI mediated the relationship between PD and NSSI, and the estimated ratio of the total effect of PD that could be explained by mediation through SI was 33.59%. The ratio was higher in female adolescents with MDD (39.23%) than in males(18.05%), and it was higher in urban adolescents with MDD(43.35%) than in rural adolescents with MDD(15.55%).Conclusions: Among adolescents with MDD, PD may influence NSSI behavior through SI. Interventions aimed at reducing NSSI associated with PD may be enhanced by efforts to mitigate SI, especially in females and urban adolescents.
Significance of the expression of adhesion moleculesin the progression of gallbladder cancer
Authors: Kim Sungho , Suh Shin-Do , Suh Sung-Ok , Lee Kun-Ok , Han Hyung-Joon , Song Tae-Jin 
Published: August 2022

Abstract
Background The role and clinical utility of the cell adhesion molecule (CAM) have been studied in several malignancies. However, there have been few studies of the role of the CAM in gallbladder cancer. The aim of this study is to evaluate the clinical utility and the value as a prognostic factor of the CAM in gallbladder cancer. MethodsThe expression of CAMs (E-cadherin, E-selectin, ICAM-1 and VACM-1) of the tissues from 25 malignant and 25 benign gallbladder disease patient who were operated between January 2005 and July 2009 at Korea University Ansan Hospital were analyzed by immunohistochemical stain and real time polymerase chain reaction (PCR). And the correlation of expression of CAMs with clinicopathological variables were evaluated.ResultsThe expressions of E-cadherin, ICAM-1 and VACM-1 on immunohistochemical stain of malignancy group were decreased with statistically differences compared to benign group (p<0.001, p=0.01 and p=0.001, respectively). The ratio of E-selectin and ICAM-1 in the gallbladder cancer tissues was significantly decreased on real time PCR (p=0.009 and p=0.034, respectively), and the alteration of expression in E-selectin was significantly correlated to cell differentiation(p=0.023), T stage(p=0.005) and TNM stage(p=0.023).ConclusionsThis result could be a base of the alteration of expression of CAMs and suggests the possibility that E-selectin may be used in prediction of outcomes of gallbladder cancer and expected to be used in practice.
Specific lncRNA signatures discriminate childhood acute leukemias: a pilot study
Authors: Buono Lorena , Iside Concetta , Matteo Antonia , Stellato Pio , Beneduce Giuliana , Penta Roberta , Parasole Rosanna , Salvatore Marco , Smaldone Giovanni , Mirabelli Peppino 
Published: August 2022

Abstract
Background: Long non-coding RNAs are RNAs longer than 200 bps that do not encode any proteins and are able to alter gene expression by acting on different steps of regulation, including DNA methylation and chromatin structure. They represent a class of biomarkers of crescent interest in the hematologic and oncologic fields. Recent studies showed that the expression levels of specific lncRNAs correlate with the prognosis of paediatric patients with Acute Lymphoblastic Leukaemia. Methods: We used NGS approaches to analyse the transcriptome of 9 childhood B-ALL patients and 6 childhood T-ALL patients, in comparison with B and T healthy lymphocytes from cord blood. We validate our findings both ex vivo, in a different cohort of 10 B-ALL and 10 T-ALL patients, and in silico using public datasets.Results: We characterised the lncRNA landscape for B-ALL, T-ALL, healthy B, and T cell progenitors. From the characterised signature, we selected candidate lncRNAs able to discriminate not only B-ALL and T-ALL from healthy subjects but also between the two types of leukaemia, and subsequently validated their potential as a diagnostic tool in an additional cohort of paediatric patients. We confirmed our finding with open access transcriptomic data, comparing ALL lncRNAs with AML lncRNA landscape as well. Finally, expression correlation analyses of T-ALL selected lncRNA biomarkers suggested a possible role in lymphocyte activation and the β-catenin signalling pathway for AC247036.1 and involvement in hedgehog signalling for HHIP-AS1.Conclusions: Our work identified a lncRNA signature discriminating paediatric B-ALL and T-ALL from healthy subjects, between them and from AML. This study provides the keystone to future clinical studies determining the theragnostic value of the characterised long non coding transcriptome panorama in a clinical setting for childhood patient management.
A novel single layer full-adder in Quantum-dot Cellular Automata (QCA)
Authors: Rezaei Fatemeh , Omidi Reza 
Published: August 2022

Abstract
Quantum-dot Cellular Automata (QCA) is a new technology for designing digital circuits in nanoscale. The design of a QCA circuit is radically different from the conventional digital design due to its unique characteristics at both the physical and logical levels. Quantum-dot Cellular Automata is not a physical implementation yet, it is rather a lower-level abstraction since there are several ways to build the quantum dots and connect them. Quantum dots can be any charge containers, with discrete electrical energy states (there may be more than two states, but only two-state case is used), sometimes called artificial atoms. In this paper, a new 3-input majority gate was initially proposed, then an efficient design of a single-layer full-adder was introduced using the proposed optimized 3-input majority gate. The functional correctness of the proposed structure is evaluated by the QCA Designer tool. Simulation results show that the proposed full-adder is improved and more efficient in terms of area, the number of cells, and latency.
The Diagnostic Performance of Antimullerian Hormone for Polycystic Ovarian Syndrome and Polycystic Ovarian Morphology
Authors: Vural Fisun , Vural Birol , Kardaş Eyupcan , Coşkun Ayşe Deniz Ertürk , Yildirim İrem 
Published: August 2022

Abstract
Purpose: The diagnosis of polycystic ovary syndrome (PCOS) remains a challenge to clinicians due to heterogeneous clinical presentation and diagnostic criteria. This study investigated the utilization of Anti-Müllerian hormone (AMH) alone or replacing polycystic ovarian morphology (PCOM) in the PCOS diagnostic criteria.Methods: A total of 401 women were categorised as PCOS (n:154), nonPCOS with polycystic ovarian morphology (PCOM) (n:105), and nonPCOS with normal ovarian morphology (NOM) (n:142). Firstly, diagnostic performance of AMH for PCOS diagnosis in Rotterdam, Androgen Excess Society, and National Institutes of Health (NIH) criteria was analyzed. Secondly, AMH was used instead of PCOM in Rotterdam criteria and we searched diagnostic performance for PCOS phenotypes Results: AMH levels were positively correlated with LH, testosterone, hirsutism score, menstrual cycle length, and antral follicle count (p<0.05). AMH alone had specificity and sensitivity for PCOS diagnosis were 84.9% and 72.4% in Rotterdam (AUC: 0,866) ; 84.4 % and 72% in Androgen Excess Society (AUC:0.857) ; 83.3 % and 66.4% in National Institute of Health criteria (AUC:0.825). AMH alone had satisfactory diagnostic potential for phenotype A, but not other phenotypes. The replacement of PCOM with AMH in Rotterdam criteria had high diagnostic potential for PCOS (AUC:0.934, sensitivity:97.4%,specificity: 90.67%).Phenotype A and phenotype D were diagnosed with 100% sensitivity and 94.5 % specificity. Phenotype C was recognised with 96.15 % sensitivity and 94.5 % specificity.Conclusion: AMH may be used with high diagnostic accuracy instead of PCOM in the Rotterdam PCOS criteria.
Hydrogeochemistry and hydrogen and oxygen stable isotope characteristics of subsidence lakes in coal mining areas with high phreatic levels in eastern China
Authors: jiang chunlu , Jiang Chenghong , Zha Junzhen , Liu Hui , Liu Dou , Zheng Liugen 
Published: August 2022

Abstract
Large-area subsidence lakes in coal mining areas with high phreatic levels in eastern China play a very important role in regulating water resources and improving the ecological environment. These coal mining-related subsidence lakes vary in formation time and connectivity with rivers. These factors may influence the hydrogeochemistry and hydrogen and oxygen stable isotope characteristics of the lake water. This study assessed the hydrogeochemical and hydrogen and oxygen stable isotope characteristics and the influencing factors of the subsidence lake water, surface river water and shallow groundwater in the Huainan coal mine area in eastern China. The results showed that the hydrochemical types of the subsidence lake water and river water are Cl-Na and HCO3•Cl-Na and that the hydrochemical types of the shallow groundwater are mainly HCO3•Cl-Na and HCO3•Cl-Ca. There are no significant differences in the hydrochemical characteristics of subsidence lakes with different subsidence ages and types. The major ions in each water body mainly come from evaporite dissolution and silicate weathering, and ion exchange occurs. Reverse ion exchange occurs in some shallow groundwater samples. The hydrogen and oxygen isotopic compositions of the subsidence lake water, river water and shallow groundwater plot below and to the right of the LMWL and are distributed along a straight line, indicating that these water bodies have a common source, namely, precipitation. With increases in the formation time of the subsidence lakes, the heavy isotopes in the lake water gradually become depleted, and the d value gradually increases, mainly driven by precipitation dilution, weakening evaporation, river recharge and groundwater recharge. The isotopic values of different types of lakes with the same subsidence time differ little. The research results are of great significance for understanding the water cycle and water resource evaluation of coal mining subsidence lakes.
Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein
Authors: Grignano Eric , Cantero-Aguilar Lilia , Tuerdi Zubaidan , Chabane Thella , Vazquez Romain , Johnson Natacha , Birsen Rudy , Fontenay Michaela , Kosmider Olivier , Chapuis Nicolas , Bouscary Didier 
Published: August 2022

Abstract
Artemisinin is an anti-malarial drug that has shown anticancer properties. Recently, ferroptosis was reported to be induced by dihydroartemisinin and linked to iron increase. In the current study, we determined the effect of artemisinin in leukemic cell lines on ferroptosis induction and iron metabolism and the cytoprotective effect triggered in leukemic cells. We found that treatment of artemisinin induces early ferroptosis by promoting ferritinophagy and subsequent iron increase. Furthermore, our study demonstrated that artemisinin activated zinc metabolism signaling, especially the upregulation of metallothionein. By inhibiting MT2A and MT1M isoforms, we showed that cytotoxic effect of artemisinin and ferroptosis induction were enhanced. Finally, we demonstrated that ferroptosis inducers effect acting on glutathione pool were highly dependent on MTs-driven antioxidant response. Taken together, our study indicated that DHA activates ferritinophagy and subsequent ferroptosis in AML and that MTs are involved in glutathione regenerating and antioxidant response.
Pan-specific Multi Allelic pHLA Presenting Prediction through Resnet-based and LSTM-based Neural Networks
Authors: Mi Xue , Li Shaohao , Ye Zheng , Dai Zhu , Feng Songwei , Li Zhanping , Yan Kai , Shao Baoyi , Ding Bo , Sun Bo , Shen Yang , Xiao Zhongdang 
Published: August 2022

Abstract
Peptide-HLA (pHLA) is a peptide that may attach to human leukocyte antigen (HLA) and be presented to specialized immune cells, then initiate an immune response. Computational prediction of peptide and HLA binding is an important tool in studying T cell immunity that can assist in the design of neoantigen vaccines. However, the majority of current prediction approaches are limited to single allele (SA) HLA data, predictive tools to optimize mutant peptides with higher affinity for multi allelic (MA) HLA are lacking. Here, we describe ResMAHPan (https://github.com/Luckysoutheast/ResMAHpan.git), which integrates long short term memory (LSTM) network and Resnet network with coordinate attention (CA) for pHLA binding and presentation prediction. ResMAHPan considerably outperforms the standard predictors NetMHCpan 4.0 and MHCflurry 2.0 by enriching for current MA HLA presentation prediction algorithms on held-out mass spectrometry experiments. We propose a mode based on existing MA-pHLA encoding that allows incorporation of neoantigen prediction tasks into computer vision methods—which can aggregate MA HLA molecules into a multichannel matrix and incorporated peptide sequences to capture binding signals efficiently. Finally, the integrated model could be employed as a independent neoantigen recognition approach to improve neoantigen identification accuracy, or in conjunction with other methods to achieve the maximum level of accuracy.
Wild kras inhibit the migration and invasion of pancreatic cancer through Wnt /β-catenin pathway
Authors: hu xianhua , Zhao Chunyan , Zhang Rendan , yao jiaxin , Bo Mu 
Published: August 2022

Abstract
introduction:Kras mutation is considered to be the initiation event of PDAC, and the mutation frequency of kras gene in PDAC is 90%-95%. Studies have shown that wild kras has a survival advantage in PDAC and can antagonize the effect of krasG12D, leading to low cell transformation efficiency. This study focused on the differences in biological behavior between wild kras and krasG12D, and explored the mechanism of its action on pancreatic cancer.Materials and methods: we transfected overexpressed wild kras and mutated kras into cells by lentivirus transfection. The differences and mechanisms were explored by CCK-8, clone formation assay,wound healing assay,transwell test,western blot,immunohistochemistry, and tumor formation in nude mice.Results: In vitro, the proliferation of wild kras was reduced compared with the control, while the proliferation of mutant kras was not significantly changed. In vivo, the proliferation of wild kras was reduced and the proliferation of mutated kras was enhanced compared to the control. The invasion and migration of wild kras decreased, while the invasion and migration of mutant kras increased.western blot showed that the expressions of E-cadherin, α-E-catenin, MMP-3, MMP-9 and p-STAT3 in wild kras were up-regulated, while the mutant kras had no significant change. The results of immunohistochemistry were consistent with those of western blot. Conclusion: wild kras can inhibit proliferation of pancreatic cancer in vitro and in vivo, while mutant kras can promote proliferation in vivo, but not significantly in vitro.wild kras may inhibit invasion and migration of pancreatic cancer through the Wnt /β-catenin pathway.
Development of machine learning-based reference evapotranspiration model for the semi-arid region of Punjab, India
Authors: Das Susanta , KAUR SAMANPREET , Raheja Amina , Gill Kulwinder Kaur , Sharda Rakesh 
Published: August 2022

Abstract
Evapotranspiration (ET) is a critical element of the hydrological cycle, and its proper assessment is essential for irrigation scheduling, agricultural and hydro-meteorological studies, and water budget estimation. It is computed for most applications as a product of reference crop evapotranspiration (ET0) and crop coefficient, notably using the well-known two-step method. Accurate predictions of reference evapotranspiration (ET0) using limited meteorological inputs are critical in data-constrained circumstances, and the preferred FAO-56 Penman-Monteith (PM) equation cannot be used. To overcome the complexity of calculation, the present study is focused on developing a Random Forest-based ET0 model to estimate the crop ET for the semi-arid region of northwest India. The RF-based model was developed by focusing on the readily available data at the farm level. For comparative study Hargreaves–Samani model was also modified and used to estimate the ET0. Further, ET0 was also estimated using existing models like Hargreaves–Samani model and the Modified Panman model. The models' calibration and validation were done using meteorological data collected from the weather station of Punjab Agricultural University for 21 years (2090 − 2010) and nine years (2011–2019), respectively, and the PM FAO-56 model was taken as a standard model. The developed RF-based model's mean absolute error and root-mean-square deviation were found to be better than the other models, and it was obtained as 0.95 mm/d and 1.32, respectively, with an r2 value of 0.92. The developed RF-based model was used to predict the ET0, and further, predicted ET0 values were used for irrigation scheduling of two growing seasons (2020–2021) of maize and wheat crops. The result of the field experiment also shows that there was no significant yield reduction in the crop. Hence, This developed study model can be used for the irrigation in the semiarid region of the Punjab India as well as other part of world. Also, it can be used as a replacement FAO-56 model.
Umbilical cord-derived mesenchymal stromal cell therapy to prevent the development of neurodevelopmental disorders related to low birth weight
Authors: Tsuji Masahiro , Mukai Takeo , Sato Yoshiaki , Azuma Yasue , Yamamoto Saki , Cayetanot Florence , Bodineau Laurence , Onoda Atsuto , Nagamura-Inoue Tokiko , Coq Jacques-Olivier 
Published: August 2022

Abstract
Low birth weight (LBW) increases the risk of neurodevelopmental disorders (NDDs), such as attention-deficit/hyperactive disorder and autism spectrum disorder, as well as cerebral palsy. Neuroinflammation in fetuses and neonates plays a major pathogenic role in NDDs. Human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) have immunomodulatory properties. LBW pups born to dams subjected to mild intrauterine hypoperfusion and pups born to sham-treated dams, received intravenous administration of either human UC-MSCs (1 × 105 cells) or a vehicle on postnatal day 1 (P1). Rats in the LBW-vehicle group exhibited significantly lesser decrease in the monosynaptic response with increased frequency of stimulation to the spinal cord preparation from P4 to P6, suggesting hyperexcitability, which was improved by UC-MSC treatment. Three-chamber sociability tests at 7 weeks of age showed that only males in the LBW-vehicle group exhibited disturbed sociability, which was ameliorated by UC-MSC treatment. Other parameters, including those determined via open-field tests, were not improved by UC-MSC treatment. Serum or cerebrospinal fluid levels of pro-inflammatory cytokines were not elevated in the LBW-vehicle group, and the effects of UC-MSC treatment on inflammation were unclear. In conclusion, UC-MSC treatment may prevent the development of NDDs.