bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiological and transcriptomic analysis of OsLHCB3 knockdown lines in rice
Authors: Wang Quanxiu , Chen Pingli , Wang Honglin , Chao Shuangshuang , Guo Wenru , Zhang Yuxue , Miao Chenglin , Yuan Hongyu , Peng Bo 
Published: November 2022

Abstract
The photosystem II (PSII) outer antenna OsLHCB3 protein plays critical roles in binding PSII chlorophyll, absorbing sunlight and transfering the excitation energy for photosynthesis. Here, OsLHCB3 knockdown mutants were produced using the RNAi system. Phenotypic analyses showed that OsLHCB3 knockdown led to pale green leaves and lower chlorophyll contents at both tillering and heading stages. In addition, mutant lines exhibited decreased non-photochemical quenching (NPQ) capacity and net photosynthetic rate (Pn) by downregulating the expression of PSII-related genes. Moreover, RNA-seq experiments were performed at both tillering and heading stages. The diferentially expressed genes (DEGs) mainly involved in chlorophyll binding, response to abscisic acid, photosystem II, response to chitin, and DNA-binding transcription factor. Besides, our transcriptomic and physiological data indicated that OsLHCB3 was essential for binding chlorophyll, but not for the metabolism of chlorophyll in rice. OsLHCB3 RNAi knockdown plants affected the expression of PS II-related genes, but not PS I-related genes. Overall, these results suggest that OsLHCB3 also plays vital roles in regulating photosynthesis and antenna proteins in rice as well as responses to environment stresses.
MicroRNA-326 Negatively Regulates CD155 Expression in Lung Adenocarcinoma
Authors: Nakanishi Takayuki , Yoneshima Yasuto , Okamura Koji , Hashisako Mikiko , Haratake Naoki , Ota Keiichi , Iwama Eiji , Takenaka Tomoyoshi , Tanaka Kentaro , Yoshizumi Tomoharu , Oda Yoshinao , Okamoto Isamu 
Published: November 2022

Abstract
Treatment with immune checkpoint inhibitors induces a durable response in some patients with non–small cell lung cancer, but eventually gives rise to drug resistance. Upregulation of CD155 expression is implicated as one mechanism of resistance to PD-1/PD-L1 inhibitors, and it is therefore important to characterize the mechanisms underlying regulation of CD155 expression in tumor cells. The aim of this study was to identify microRNAs (miRNAs) that might regulate CD155 expression at the posttranscriptional level in lung cancer. Comprehensive miRNA screening with target prediction programs and a dual-luciferase reporter assay identified miR-346, miR-328-3p, miR-326, and miR-330-5p as miRNAs that bind to the 3′ untranslated region of CD155 mRNA. Forced expression of these miRNAs suppressed CD155 expression in lung cancer cell lines. Immunohistochemical staining of CD155 in tissue specimens from 57 patients with lung adenocarcinoma revealed the median tumor proportion score for CD155 to be 68%. The abundance of miR-326 in these specimens with a low level of CD155 expression was significantly greater than that in those with a high level (P < 0.005). Our results thus suggest that miR-326 negatively regulates CD155 expression in lung adenocarcinoma and might therefore play a role in the development of resistance to PD-1/PD-L1 inhibitors.
High efficiency and stability due to reduced effects of long-lived charge formation and accumulation in organic solar cells
Authors: Kamiya Shinpei , Yamaguchi Seira , Watanabe Takahiro , Saito Masahiko , Osaka Itaru , Marumoto Kazuhiro 
Published: November 2022

Abstract
The performance of organic solar cells has remarkably improved recently, where the cell structures are important for achieving high efficiency and stability. The formation and accumulation of long-lived charges in the cells are critical for the efficiency and stability of the cells; however, their relations with the cell structures have not yet been investigated from a microscopic viewpoint. Here, we show the origin of higher efficiency and stability of inverted organic solar cells compared to those of conventional cells, even though the same photoactive layers with fullerene acceptors are utilized. We directly study the formation and accumulation of long-lived charges in these cells with electron spin resonance (ESR) at a molecular level for as-prepared states and operando states. We find the reduced effects of formation and accumulation of long-lived charges for the efficiency and stability in the inverted cells compared to the case of the conventional cells. These findings are useful for deeply understanding the operation mechanism of organic solar cells as well as further improving their efficiency and stability.
Reference values of diaphragmatic dimensions in healthy children aged 0-8 years
Authors: Duyndam Anita , Smit Joke , Heunks Leo , Molinger Jeroen , IJland Marloes , Rosmalen Joost van , Dijk Monique van , Tibboel Dick , Ista Erwin 
Published: November 2022

Abstract

Purpose

Diaphragmatic thickness (Tdi) and diaphragm thickening fraction (dTF) are widely used parameters in ultrasound studies of the diaphragm in mechanically ventilated children, but normal values for healthy children are scarce. We determined reference values of Tdi and dTF using ultrasound in healthy children aged 0–8 years old, and assessed their reproducibility.

Method

In a prospective, observational cohort, Tdi and dTF were measured on ultrasound images across four age groups compromising at least 30 children per group: group 1 (0–6 months); group 2 (7 months-1 year); group 3 (2–4 years); group 4 (5–8 years).

Results

Ultrasound images of 137 healthy children were included. Mean Tdi at inspiration was 2.07 (SD 0.39), 2.09 (SD 0.38), 1.73 (SD 0.33) and 1.70 (SD 0.30) mm for group 1, 2, 3 and 4 respectively. Mean Tdi at expiration was 1.63 (SD 0.33), 1.67 (SD 0.27), 1.40 (SD 0.23) and 1.40 (SD 0.24) mm for group 1, 2, 3 and 4 respectively. Mean Tdi at inspiration and mean Tdi at expiration for groups 1 and 2 were significantly greater than those for groups 3 and 4 (p < 0.001 respectively). Median dTF was 27.1% (SD 12.5), 24.7% (SD 8.6), 24.1% (SD 10.4) and 21.2% (SD 6.8) for group 1, 2, 3 and 4 respectively. The intraclass correlation coefficients for intra-rater and inter-rater reliability were 0.995 (95% CI 0.983–0.999) and 0.989 (95% CI 0.983–0.995), respectively.

Conclusion

Ultrasound measurements of Tdi and dTF were highly reproducible in healthy children aged 0–8 years.
ClinicalTrials.gov identifier (NCT number): NCT04589910
North Carolina Pharmacists’ Willingness to Sell Fentanyl Test Strips: A Survey Study
Authors: Marley Grace T. , Ostrach Bayla , Carpenter Delesha M. 
Published: November 2022

Abstract
Background
Although fentanyl test strips (FTS) can accurately determine the presence of fentanyl in unregulated substances, access to FTS remains limited. This study aimed to examine North Carolina community pharmacists’ attitudes and willingness to engage in various behaviors related to FTS sales and distribution.
Methods
A convenience sample of community pharmacists completed an online survey that assessed: (1) comfort initiating a FTS conversation; (2) willingness to sell FTS, distribute FTS instructions, counsel on FTS, refer patients to harm reduction organizations, and advertise FTS; (3) perceived barriers and benefits of selling FTS; and (4) interest in FTS training. Data were collected from March to May 2022. Descriptive statistics were calculated.
Results
Of the 592 pharmacists who participated, most were somewhat or very willing to refer patients to harm reduction organizations for FTS (514, 86.9%), counsel on FTS (485, 81.9%), distribute FTS instructions (475, 80.2%), sell FTS (470, 79.3%), and advertise FTS for sale (372, 62.9%). The most commonly reported benefits of selling FTS was reducing overdose deaths in the community (n = 482, 81.4%) and participating in community harm reduction efforts (n = 455; 76.9%). Barriers commonly reported to selling FTS were: not knowing where to order FTS (n = 295, 49.8%) and discomfort initiating a conversation about FTS (n = 266, 44.9%). Most respondents (88.3%) were interested in FTS training.
Conclusion
North Carolina community pharmacists are willing to engage in various behaviors related to FTS sales and distribution. Pharmacists may benefit from FTS training that focuses on barriers to FTS sales. Pharmacist distribution of FTS could increase access to FTS at the community level, and has the potential to change drug use behavior and reduce overdose deaths.
Comparison of Prediction Model of Cooperation Risk of Multinational Enterprises Based on Deep Learning for International Policies
Authors: Guo Congying 
Published: November 2022

AbstractThe purpose is to study the effective risk management mode in the process of transnational cooperation of enterprises under the background of international policy and optimize the international cooperation strategy of enterprises. Firstly, the current situation of cross-cultural enterprise management models is compared and explored. Secondly, the advantage of efficient task offloading of edge cloud computing is used to extract the data information of multinational enterprises. Based on the extracted data, the loyalty of enterprise management is predicted through the deep learning (DL) model, and the prediction model of cooperation risk of multinational enterprises is implemented. Finally, the theory of game theory is applied in the calculation of profit distribution among multinational alliance enterprises, and the index evaluation system is established for quantitative and qualitative analysis. The results reveal that after 300 iterations, the accuracy loss of the traditional model is reduced to 0.3, while the accuracy loss of the prediction model established in this work is reduced to 0.15. Through the comparison between different models, it can be found that DL has an excellent effect on the risk prediction of enterprise strategic cooperation. This reveals that the key to transnational cooperation is to enhance the management ability of enterprises, thereby improving the competitiveness of enterprises. It can be concluded that DL and game theory are of practical value for reasonable resource allocation in transnational cooperation, to improve the risk management and prediction ability of enterprises.
Expression of glycerol-3-phosphate acyltransferase increases neutral lipid accumulation in Nannochloropsis oceanica
Authors: Sudfeld Christian , Kiyani Aamna , Wefelmeier Katrin , Wijffels Rene , Barbosa Maria , D’Adamo Sarah 
Published: November 2022

Abstract
Microalgae are considered a suitable production platform for high-value lipids and oleochemicals. Several species including Nannochloropsis oceanica produce large amounts of essential \(\omega\)-3 polyunsaturated fatty acids (PUFAs) which are integral components of food and feed and have been associated with health-promoting effects. N. oceanica can further accumulate high contents of neutral lipids with chemical properties that render them a potential replacement for plant oils such as palm oil. However, biomass and lipid productivities obtained with microalgae need to be improved to reach commercial feasibility. Genetic engineering can improve biomass and lipid productivities, for instance by increasing carbon flux to lipids. Here, we report the overexpression of two glycerol-3-phosphate acyltransferases (GPAT) in N. oceanica during favorable growth conditions as a strategy to increase neutral lipid content. Transformants overproducing either an endogenous (NoGPAT) or a heterologous (AoGPAT) GPAT enzyme targeted to the endoplasmic reticulum, had up to 42% and 51% increased neutral lipid contents, respectively, compared to the wild type. Biomass productivities of transformant strains were not substantially impaired, resulting in lipid productivities that were increased by up to 37% and 42% for NoGPAT and AoGPAT transformants, respectively. When exposed to nutrient stress, transformants and wild type had similar lipid contents, suggesting that GPAT enzyme availability is a rate-limiting factor for lipid synthesis in N. oceanica under favorable growth conditions. NoGPAT transformants further accumulated PUFAs in neutral lipids, reaching a total of 6.8% PUFAs per biomass, an increase of 24% relative to the wild type. Overall, our results indicate that GPAT is an interesting target for engineering of lipid metabolism in microalgae, in order to improve neutral lipid and PUFA accumulation in microalgae.
TERT promoter mutation predicts the prognosis value of patients with HBV- related HCC who underwent hepatectomy
Authors: Aizimuaji Zulihumaer , Zhao Huan , Wang Xijun , Ma Sheng , Wang Yaru , Rong Weiqi , Xiao Ting 
Published: November 2022

AbstractBackground and AimsRecurrence is the major cause of poor prognosis in hepatocellular carcinoma (HCC), especially in those infected with HBV. Telomerase reverse transcriptase (TERT) promoter is the most prevalent site in HBV-related HCC. We attempted to evaluate the prognostic value of TERT mutation status in HBV-related HCC patients.MethodsWe enrolled the 66 HBV-related HCC patients who underwent hepatectomy between January 2013 and May 2016. DNA extracted from the fresh tumor tissues of these patients was subjected to Sanger sequencing and optimized droplet polymerase chain reaction (dPCR) for detecting TERT promoter mutation. We optimized the dPCR assay by adding 7-deaza-dGTP, CviQ1, and EDTA for improving the detection of TERT promoter mutation. Furthermore, nomogram survival prediction models were developed to assess the prognostic value of HBV-related HCC after hepatectomy.ResultsThe frequency of TERT C228T mutation was detected 24/66 (36.36%) by Sanger sequencing and 30/66 (45.45%) by dPCR. The two methods had an initial overall agreement(concordance = 90.91%, kappa = 0.814; dPCR sensitivity, 100%; dPCR specificity, 85.71%). HBV-related HCC patients with TERT C228T mutation had reduced survival and increased risk of recurrence. TERT C228T was an independent prognostic factor for OS and DFS in multivariate analyses. The nomograms successfully differentiated TERT C228T mutant and non-mutant HBV-related HCC patients associated with OS and DFS (C-index: 0.7468 and 0.6899, respectively).ConclusionsTERT C228T mutation could be a potential molecular biomarker for evaluating the prognosis of HBV-related HCC, especially when it is detected by dPCR rather than traditional Sanger sequencing. The nomogram survival prediction model we constructed may provide a valuable reference for clinicians to develop strategies for precision treatment.
MiR-760 is a key regulator of glioma proliferation migration and invasion by controlling MMP2 expression
Authors: qian zhengting , Xin Heng , Jia Zhen , Xia Jiageng , Tang Yong , Li Xiang , Wu Heming , Fan Youwu 
Published: November 2022

Abstract
Background: Glioma is the most common kind of brain tumor and has a poor prognosis. As available evidence indicates, miRNAs are involved in the origin and development of glioma malignancies. MiR-760 has been reported to the development of tumors, but miR-760 has not been well studied in gliomas.
Methods: In this study, we used database of GSE25631 to survey the abnormal expression of microRNA, and the expression of miR-760 validated decreased in glioblastoma. We sought to identify the microRNA-760 (miR-760) expression pattern and investigate its prognostic significance in glioma. We used quantitative real-time polymerase chain reaction to determine the relative expression of miR-760 and MMP2 in glioma cell lines. The effect of miR-760 on cell proliferation, migration, and invasion was determined using the Cell Counting Kit-8 (CCK-8), the 5-ethynyl-2'-deoxyuridine (EdU) assay, and the transwell assay. Bioinformatics analysis confirmed that the downstream target gene of miR-760. Additionally, a luciferase reporter experiment was used to identify the miR-760 target gene MMP2. Western blot and immunofluorescence was used to determine MMP2 protein levels.
Result: Our data demonstrated that miR-760 expression was significantly decreased in glioma tissues and cell lines. Increased miR-760 expression induced proliferation, migration, and invasion in glioma cell lines. Bioinformatics analysis showed that miR-760 binds and inhibits MMP2 expression. These results suggest that miR-760 inhibits glioma growth by up-regulating the expression of miR-760 along the miR-760 /MMP2 axis.
Conclusion: The finding of the miR-760/MMP2 axis will expand our insights into the molecular mechanisms underlying glioma growth and will identify potential therapeutics for glioma treatment.
A hard molecular nanomagnet from confined paramagnetic 3d-4f spins inside a fullerene cage
Authors: Huang Chenli , Sun Rong , Bao Lipiao , Tian Xinyue , Pan Changwang , Li Mengyang , Shen Wangqiang , Guo Kun , Wang Bingwu , Lu Xing , Gao Song 
Published: November 2022

Abstract
Reducing inter-spin distance can enable effective magnetic interactions to realize outstanding magnetic properties, which, however, remains rather challenging. Here, an unprecedented 3d-4f metal cluster (Dy2VN) was constructed inside a C80 cage, affording a heretofore unseen metallofullerene containing both paramagnetic 3d and 4f metal ions. Significantly suppressed 3d-4f (Dy-V) distances, due to the unique cage confinement effect, are directly recorded by crystallographic and theoretical analysis of Dy2VN@Ih(7)-C80. Consequently, superior magnetic coupling (Jtotal, Dy-V = 53.30 cm-1; Jtotal, Dy-Dy = -18.75 cm-1) is obtained, leading to a record-high magnetic blocking temperature among all reported 3d-4f single-molecule magnets and huge coercive field of 2.73 Tesla. Our work puts forth a new class of single-molecule magnets with both paramagnetic 3d and 4f metals confined in a fullerene cage, of which superior and tunable magnetic properties are envisioned thanks to the unique cage confinement effect and the diverse composition of the entrapped magnetic core.
A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva
Authors: Wagner Kaitlyn , Fox Phil , Gordon Elizabeth , Hahn Westen , Olsen Kenzie , Markham Alex , Buglewicz Dylan , Selemenkis Platon , Lessard Avery , Goldstein Daniella , Threatt Alissa , Davis Luke , Miller-Dawson Jake , Stockett Halie , Rugh Kristin , Turner Houston , Remias Michelle , Williams Maggie , Chavez Jorge , Galindo Gabriel , Cialek Charlotte , Koch Amanda , Fout Alex , Fosdick Bailey , Broeckling Bettina , Zabel Mark 
Published: November 2022

Abstract
In response to the SARS-CoV-2 pandemic, we developed a multiplexed, paired-pool droplet digital PCR (MP4) screening assay. Key features of our assay are the use of minimally processed saliva, 8-sample paired pools, and reverse-transcription droplet digital PCR (RT-ddPCR) targeting the SARS-CoV-2 nucleocapsid gene. The limit of detection was determined to be 2 and 12 copies per µl for individual and pooled samples, respectively. Using the MP4 assay, we routinely processed over 1,000 samples a day with a 24-hour turnaround time and over the course of 17 months, screened over 250,000 saliva samples. Modeling studies showed that the efficiency of 8-sample pools was reduced with increased viral prevalence and that this could be mitigated by using 4-sample pools. We also present a strategy for, and modeling data supporting, the creation of a third paired pool as an additional strategy to employ under high viral prevalence.
Skin lesion classification and Prediction by Data Augmentation in HAM10000 and ISIC 2019 dataset
Authors: V Auxilia osvin nancy , Arya Meenakshi S , P Prabahavathy , B Shamreen Ahamed 
Published: November 2022

Abstract
Skin lesions are a common sign of many human diseases and are a worldwide indicator of many different types of cancer. The necessity of such skin cancer preventive initiatives is highlighted by increased risks brought on by the effects of climate change and by the high expense of treatment. The early detection of Skin Cancer can be done to save many lives. Melanoma is the deadliest type of Cancer out of the known types so far. HAM 10000 and ISIC 2019 are the datasets that are used to classify seven and eight classes in the proposed article. The approach is five-layer CNN. The impact of data augmentation was analyzed using the proposed framework in two different datasets. Compared to the original data, the evaluation metrics for augmented data are high. For the ISIC 2019 and HAM10000 dataset, the CNN fine-tuned 5-layered model with augmentation achieved 98.67, 97.88 percent accuracy.
Skin lesion classification and Prediction by Data Augmentation in HAM10000 and ISIC 2019 dataset
Authors: V Auxilia osvin nancy , Arya Meenakshi S , P Prabahavathy , B Shamreen Ahamed 
Published: November 2022

Abstract
Skin lesions are a common sign of many human diseases and are a worldwide indicator of many different types of cancer. The necessity of such skin cancer preventive initiatives is highlighted by, increased risks brought on by the effects of climate change and by the high expense of treatment. The early detection of Skin Cancer can be done to save many lives. Melanoma is the deadliest type of Cancer out of the known types so far. HAM 10000 and ISIC 2019 are the datasets that are used to classify seven and eight classes in the proposed article. The approach is five-layer CNN. The impact of data augmentation was analyzed using the proposed framework in two different datasets. Compared to the original data, the evaluation metrics for augmented data are high. For the ISIC 2019 and HAM10000 dataset, the CNN fine-tuned 5-layered model with augmentation achieved 98.67, 97.88 percent accuracy.
Closed-form formula for some recursively-defined integro-difference sequence of functions
Authors: Yadeta Hailu Bikila 
Published: November 2022

Abstract
The main purpose of this paper is to derive the closed form solution the sequence $(g_n)_{n\in \mathbb{N}}$ of integro-difference equations that is defined recursively as follows:
\begin{align*}
g_1(x) & = \chi_{(-1/2, 1/2)} (x), \\
g_{n+1}(x) & = g_n(x + 1/2)- g_n(x- 1/2) + \int_{x-\frac{1}{2}}^{x + \frac{1}{2}} g_n(s)ds, \, n\in \mathbb{N},
\end{align*}
where $ g_1(x)= \chi_{(-1/2, 1/2)} (x) $ is the characteristic function of the unit interval $(-1/2, 1/2) $ has value equal to $ 1 $ on $(-1/2, 1/2) $ and $0$ elsewhere in $ \mathbb{R} $