bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Prognostic Model using FIGO 2018 Staging and MRI- derived Tumor Volume to Predict Long-term Outcomes in Patients with Uterine Cervical Squamous Cell Carcinoma Who Received Definitive Radiotherapy
Authors: Zang Lele , Chen Qin , Lin An , Chen Jian , Zhang Xiaozhen , Fang Yi , Wang Min 
Published: August 2022

Abstract
Background
Uterine cervical carcinoma is a severe health threat worldwide, especially in China. The International Federation of Gynecology and Obstetrics (FIGO) has revised the staging system with emphasise the strength of magnetic resonance imaging (MRI). We aim to investigate long-term prognostic factors for FIGO 2018 stage II-IIIC2r uterine cervical squamous cell carcinoma following definitive radiotherapy and establish a prognostic model using MRI-derived tumor volume.
Methods
Patients were restaged according to the FIGO 2018 staging system and randomly grouped into training and validation cohorts (7:3ratio). Optimal cutoff values of squamous cell carcinoma antigen (SCC-Ag) and tumor volume derived from MRI for the training cohort were generated. A nomogram was onstructed based on overall survival (OS) predictors, which were selected using univariate and multivariate analyses. The performance of the nomogram was validated and compared with the FIGO 2018 staging system. Risk stratification cutoff points were generated, and survival curves of low-risk and high-risk groups were compared.
Results
We enrolled 396 patients (training set, 277; validation set,119). The SCC-Ag and MRI-derived tumor volume cutoff values were 11.5 ng/mL and 28.85 cm3, respectively. A nomogram was established based on significant prognostic factors, including SCC-Ag, poor differentiation, tumor volume, chemotherapy, and FIGO 2018 stage. Decision curve analysis indicated the net benefits of our model were higher. The high-risk group had significantly shorter OS than the low-risk group in both the training (p < 0.0001) and validation sets (p = 0.00055).
Conclusions
Our nomogram predicted long-term outcomes in patients with FIGO 2018 stage II-IIIC2r uterine cervical squamous cell carcinoma. This tool could assist both gynecologic oncologists and patients in treatment planning and prognosis.
Multi-inflammatory syndrome in children (MIS-C) associated with COVID-19: A nursing perspective experience report from a high-income tertiary paediatric hospital context
Authors: Hjelmgren Henrik , Andersson Karin , Widegren Jessica , Bergman Erika , Vermé Anna , Mördrup Karina , Öhlander Marcus , Bartholdson Cecilia 
Published: August 2022

Abstract
Background
In the midst of the COVID-19 coronavirus pandemic, a new disease that affects children has arisen called multisystem inflammatory syndrome in children (MIS-C). Several research articles focusing on its medical aspects have been published, but very few have focused on nursing care. The aim of this study was therefore to describe the nursing status of children suffering from MIS-C and the experiences of registered nurses (RNs) in caring for these children in paediatric hospital inpatient care.
Methods
A mixed-method design was used, including both quantitative nursing clinical record data and qualitative interview data. Quantitative data from the clinical records were analysed using descriptive statistics. Qualitative data analysis of the interviews was conducted using both deductive and inductive approaches with content analysis.
Results
In total, 47 clinical records from children with MIS-C were investigated during January–March 2021. The mean age of the children was 8.8 years. Boys were more affected than girls. Challenges in children’s nursing status were related to circulation (fever and swelling), nutrition (great thirst and loss of appetite), pain, and psychosocial situations. When caring for children with MIS-C, nurses experienced “frustration over uncertainty of care”, “children’s illbeing” and “unavoidable procedures”.
Conclusion
This study contributes knowledge to the ongoing nursing care of children suffering from MIS-C. The results show many different areas of nursing focus, which challenges nurses and other disciplines within paediatric hospital care. One important factor when caring for these children was the use of a central venous line early in the care process, which improved the quality of care. Moreover, the care of children suffering from MIS-C demands resources and time from healthcare professionals, especially RNs, to meet caring needs and reduce illbeing.
Molecular characterization and antimicrobial susceptible profiles of Thai Mycoplasma synoviae isolates
Authors: Limpavithayakul Kriengwich , Sasipreeyajan Jiroj , Pakpinyo Somsak 
Published: August 2022

Abstract
Mycoplasma synoviae infection is mostly controlled by pathogen-free flocks’ maintenance, medication in infected flocks and vaccination in high risk flocks. The effective control strategy requires convenient assay for detecting and differentiating M. synoviae strains and the reliable drug susceptible evident for making decision in antimicrobial usage. Based on sequence analysis of partial vlhA gene in this study, Thai M. synoviae isolates collected from articular joint and respiratory tract of chickens during 2020, consisted of types E and L with 19 and 35 amino acid length, respectively, differing from MS-H vaccine strain classified as types C with 32 amino acid length. Consequently, the developed PCR-RFLP assay was definitely presented ability to detect and differentiate vaccine and Thai field strains. Furthermore, antimicrobial susceptible profiles of current Thai M. synoviae isolates was performed strong susceptible to tylosin, tylvalosin, and tiamulin at MIC50 value of 0.0391, 0.0098 and 0.0781 µg/ml, respectively; moderate susceptible to doxycycline, oxytetracycline and lincomycin-spectinomycin at MIC50 value of 0.1563, 0.1563 and 0.625 µg/ml, respectively; and resistance to enrofloxacin at MIC50 value of 10 µg/ml. Interestingly, at least three subpopulations of Thai M. synoviae isolates were presented with different MIC values; ranging 0.0195–0.625 µg/ml; from strong susceptiblity to resistance to tilmicosin.
USP14 regulates ATF2/PIK3CD axis to promote microvascular endothelial cell proliferation, migration and angiogenesis in diabetic retinopathy
Authors: He Fu-Tao , Fu Xiao-Lin , Li Mo-Han , Fu Chun-Yan , Chen Jian-Zhi 
Published: August 2022

Abstract
Background
Diabetic retinopathy (DR) is the foremost cause of blindness in diabetic patients. However, the pathogenesis of DR is complex, and no firm conclusions have been drawn so far. It has become a hot spot in ophthalmology research to deeply study the mechanism of DR pathological changes and find effective treatment options.
Methods
Human retinal microvascular endothelial cells (HRMECs) were induced using high glucose (HG) to construct DR cell model. CCK-8 assay was utilized to ascertain the viability of HRMECs. Transwell assay was manipulated to ascertain the migration ability of HRMECs. Tube formation assay was utilized to ascertain the angiogenesis ability of HRMECs. The expressions of USP14, ATF2 and PIK3CD were ascertained by qRT-PCR assay and Western blot analysis. Immunoprecipitation (IP) was manipulated to identify the relationship of USP14 and ATF2. To explore the regulatory relationship between ATF2 and PIK3CD by chromatin immunoprecipitation (ChIP) assay, along with dual-luciferase reporter gene assay.
Results
The proliferation, migration and angiogenesis of HRMECs were enhanced after high glucose treatment, and the expressions of USP14, ATF2 and PIK3CD were significantly up-regulated. Knockdown of USP14 or ATF2 inhibited cell proliferation, migration and angiogenesis in HG-induced HRMECs. USP14 regulated the expression of ATF2, and ATF2 promoted PIK3CD expression. PIK3CD overexpression attenuated the inhibitory effectiveness of USP14 knockdown on proliferation, migration and angiogenesis of DR cell model.
Conclusion
Here we revealed that USP14 regulated the ATF2/PIK3CD axis to promote proliferation, migration and angiogenesis in HRMECs upon high glucose.
Assessing the causes and consequences of gut mycobiome variation in a wild vertebrate population
Authors: Worsley Sarah F. , Davies Charli S. , Mannarelli Maria-Elena , Komdeur Jan , Dugdale Hannah L. , Richardson David S. 
Published: August 2022

Abstract
Background
Considerable research has focussed on the importance of bacterial communities within the vertebrate gut microbiome (GM). However, studies investigating the significance of other microbial kingdoms, such as fungi, are notably lacking, despite their potential to influence host processes. Here, we characterise the fungal GM of individuals living in a natural population of Seychelles warblers (Acrocephalus sechellensis). We evaluate the extent to which fungal GM structure is shaped by environment and host factors, including genome-wide heterozygosity and variation at key immune genes (Major histocompatibility Complex (MHC) and Toll-like receptor (TLR) genes). Importantly, we also explore the relationship between fungal GM differences and subsequent host survival. To our knowledge, this is the first time that the genetic drivers and fitness consequences of fungal GM variation have been characterised for a wild vertebrate population.
Results
Environmental factors, including season and territory quality, explain the largest proportion of variance in the fungal GM. In contrast, neither host age, sex, genome-wide heterozygosity, or TLR3 genotype were associated with fungal GM differences in Seychelles warblers. However, the presence of four MHC-I alleles and one MHC-II allele were associated with changes in fungal GM alpha diversity. Changes in fungal richness ranged from between 1 and 10 sequencing variants lost or gained; in some cases, this accounted for 20% of the fungal variants carried by an individual. In addition to this, overall MHC-I allelic diversity was associated with small, but potentially important, changes in fungal GM composition. This is evidenced by the fact that fungal GM composition differed between individuals that survived or died within seven months of being sampled.
Conclusions
Our results suggest that environmental factors play a primary role in shaping the fungal GM, but that components of the host immune system – specifically the MHC – may also contribute to variation in fungal communities across individuals within wild populations. Furthermore, variation in the fungal GM can be associated with differential survival in the wild. Further work is needed to establish the causality of such relationships and, thus, the extent to which components of the GM may impact upon host evolution.
Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome
Authors: Villoslada-Blanco Pablo , Pérez-Matute Patricia , Íñiguez María , Recio-Fernández Emma , Jansen Daan , Coninck Lander , Close Lila , Blanco-Navarrete Pilar , Metola Luis , Ibarra Valvanera , Alba Jorge , Matthijnssens Jelle , Oteo José A. 
Published: August 2022

Abstract
Purpose: Viruses are the most abundant components of the human gut microbiome with a significant impact on health and disease. The impact of human immunodeficiency virus (HIV) infection on the gut virome has been scarcely analysed and several studies suggested that integrase strand transfers inhibitors (INSTIs) are associated with a healthier gut. Thus, the objective of this work was to evaluate the effects of HIV infection and INSTIs on the gut virome composition.Methods: 26 non-HIV-infected volunteers, 15 naive HIV-infected patients and 15 INSTIs-treated HIV-infected patients were recruited and their gut virome composition was analysed using shotgun sequencing.Results: Bacteriophages are the most abundant and diverse viruses in gut. HIV infection was accompanied by a decrease in phage richness which was reverted after INSTIs-based treatment. β-diversity of phages revealed that samples from HIV-infected patients clustered separately from those belonging to the control group. Differential abundant analysis showed an increase in phages belonging to Caudoviricetes class in the naive group and a decrease of Malgrandaviricetes class phages in the INSTIs-treated group compared to the control group. Besides, it was observed that INSTIs-based treatment was not able to reverse the increase of lysogenic phages associated with HIV infection or to modify the decrease observed on the relative abundance of Proteobacteria-infecting phages.Conclusions: Our study describes for the first time the impact of HIV and INSTIs on the gut virome and demonstrates that INSTIs-based treatments are able to partially restore gut dysbiosis at the viral level, which opens several opportunities for new studies focused on microbiota-based therapies.
Performance Analysis for Molecular Communication under Feedback Channel using Multipath and Single path Technique
Authors: Ningthoujam Sanjit , Chingkheinganba Tekcham , Chakraborty Swarnendu Kumar , Elngar Ahmed A. , Chakrabarti Prasun , Chakrabarti Tulika , Praveen S Phani , Gupta Amit , Margala Martin 
Published: August 2022

Abstract
Nanomachines that can run tasks and molecular communication (MC) for interconnecting nanomachines have been made possible by advances in nanotechnology. Different techniques of Automatic Repeat Request (ARQ) protocols have been devised in MC for acquiring the necessary molecules, although retransmission of duplicate copies requires special attention. This work presents a diffusion-based MC with a feedback loop to improve MC performance. Based on the received ACK(s) or NACK(s), the Channel state determines if the state is good (G) or bad (B), and then single path or multipath procedures are used to the ‘G' and ‘B' states. The results of the MATLAB simulation illustrate how the systems compare in terms of communication rate, the possibility of complete transmission of the desired molecules, and energy consumption. When the channel state is bad, the multipath approach performs better, but when the channel state is perfect in MC, the single path strategy performs better.
A high neutrophil-to-platelet ratio is associated with hematoma expansion in patients with spontaneous intracerebral hemorrhage: a retrospective study
Authors: Li Yujian , Yang Xiang , Zhou Huiqing , Hui Xuhui , Li Hao , Zheng Jun 
Published: August 2022

Abstract
Background
Early hematoma expansion (HE) occurs in 20 to 40% of spontaneous intracerebral hemorrhage (ICH) patients and is a primary determinant of early deterioration and poor prognosis. Previous studies have shown that inflammation is a major pathological feature of ICH, and the neutrophil-to-platelet ratio (NPR) is a marker of systemic inflammation. Therefore, we aimed to assess the association between the NPR and HE in ICH patients.
Methods
We retrospectively collected and analyzed data from ICH patients who received treatment at our institution from January 2018 to November 2019. The NPR was calculated from the admission blood test. Brain computed tomography (CT) scans were performed at admission and repeated within 24 hours. Hematoma growth was defined as relative growth > 33% or absolute growth > 6 ml.
Results
A total of 317 patients were enrolled in our study. Multivariate logistic regression analysis indicated that the NPR was an independent predictor of HE [odds ratio (OR) = 1.742; 95% CI: 1.508–2.012, p < 0.001]. Receiver operating characteristic (ROC) curve analysis revealed that the NPR could predict HE, with an area under the curve of 0.838 (95% CI, 0.788–0.888, p < 0.001). The best predictive cut-off of the NPR for HE was 5.47 (sensitivity, 75.3%; specificity, 77.6%).
Conclusions
A high NPR was associated with an increased risk of HE in patients with ICH.
High-Dose Polyethylene Glycol-3350 and Gatorade Solutions for Patients with Previous Inadequate Bowel Preparations for Colonoscopy are Safe and Effective
Authors: Gerard David , Gerard Sophia , Raiser Manfred 
Published: August 2022

Abstract
Background: Inadequate bowel preparation for colonoscopy remains an issue resulting in lower adenoma detection rates and increased cost. We assessed the efficacy, safety and tolerability of high-dose bowel preparations in subjects who previously had an inadequate colonoscopy preparation.Methods: We performed a multi-step prospective trial of high-dose bowel preparations with subjects assigned to the dose higher than their previous inadequate preparation. Step 1: 1.5 times the standard-dose of polyethylene glycol 3350 (PEG, 459 g) and Gatorade; and Step 2: 2.0 times the standard-dose of PEG (612 g) and Gatorade, both were given as extended split-dose preparations. 69 outpatients consumed their preparation before a morning colonoscopy. The primary endpoint was colon cleanliness assessed by the Chicago Bowel Preparation Scale (BPS). Safety was assessed by comparing a baseline basic metabolic panel (BMP) to a post-cleansing BMP. Patients with no history of inadequate colon cleansing who consumed standard doses of PEG (306 g to 357 g) and Gatorade were used as a comparison group. Tolerability of the bowel preparation was assessed using a subject-questionnaire.Results: When compared to controls consuming standard-dose bowel preparations, subjects consuming high-dose preparations had no statistically significant difference in colon cleanliness as measured by the modified or total Chicago BPS scores or differences in tolerability. Baseline and post-cleaning BMPs were not significantly different other than the BUN falling (p<0.0001) after the preparation.Conclusions: The multi-step high-dose bowel cleansing protocol proved highly efficacious, safe and well tolerated in subjects who previously had an inadequate colonoscopy preparation.Trial Registration: ClinicalTrials.gov NCT02661750
Expression and roles of interferon-induced protein with tetratricopeptide repeat (IFIT) genes in head-and-neck squamous cell carcinoma
Authors: Zhang Hao , Shan Fayu , Chen Gang , Wang Xinxing 
Published: August 2022

Abstract
Objective To explore the expression and roles of interferon-induced protein with tetratricopeptide repeats (IFITs) genes in head-and-neck squamous cell carcinoma (HNSCC). Methods The mRNA expression levels of the IFIT family members in different cancers and normal tissues were explored by searching the ONCOMINE and GEPIA databases. The Human Protein Atlas was used to compare the protein expression of different IFITs between normal and HNSCC tissues. The cBioPortal, GEPIA, and LinkedOmics datasets were used to explore the clinicopathological parameters and prognostic value of IFITs in HNSCC patients. The migratory and invasive abilities of SCC25 cells were determined in wound healing and Transwell migration assays, respectively, after knockdown of IFIT genes with small interfering RNA. Results Both mRNA and protein expression levels of IFIT1, IFIT2, and IFIT3 were significantly increased in HNSCC compared with those in adjacent non-cancer tissue. In the LinkedOmics and GEPIA databases, high expression of IFIT1, IFIT2, and IFIT3 were not associated with the degree of malignancy and the prognostic value of HNSCC. Knockdown of IFIT1, IFIT2, and IFIT3 had no impact on the invasion and metastasis of SCC25 cells. Conclusion HNSCC was related to high expression of IFIT1, IFIT2, and IFIT3, which might be potential biomarkers of HNSCC. However, IFIT1, IFIT2, and IFIT3 were not correlated with the migration, invasion, stage, and prognosis of HNSCC.
Push-pull behavior of 2,7-disubstituted sila- and germafluorenes
Authors: Jarrett-Noland Shelby J. , McConnell William , Braddock-Wilking Janet , Dupureur Cynthia M. 
Published: August 2022

Abstract
Push-pull dyes exhibit intramolecular charge transfer behavior, which due to changes in dipole moment upon excitation, is the origin of their sensitivity to environment. Such compounds are of interest as probes for bioimaging and as biosensors to monitor cellular dynamics and molecular interactions. Desirable biological probes absorb in the visible region, have high extinction coefficients, high quantum yield and excellent photostability. Fluorophores with scaffolding that can be used to tune and optimize solvatochromic behavior are of particular interest. Here we investigate the environmental sensitivity of a small library of highly fluorescent 2,7-disubstituted sila- and germafluorenes. Density functional theory (DFT) calculations show that charge transfer occurs from the alkyne core out to the 2,7-substitutents and 3,6-methoxy substituents, the hallmark of push-pull behavior. They exhibit a HOMO-LUMO energy gap of about 3 eV with desirable dipole moments ranging from 2-3.25 D. These compounds exhibit desirable Stokes shifts in various solvents, with absorption wavelength maxima varying up to 14 nm in polar solvents and emission wavelength maxima up to 36 nm in polar solvents compared to nonpolar solvents. Interestingly, the dependence of Stokes shift on solvent polarizability are consistent with solvatochromic behavior. With the ability to tune push-pull properties via the 2,7-substituent, these disubstituted sila- and germafluorenes have excellent potential as biological probes.
Expression of EMT-Related Genes in Lymph Node Metastasis in Endometrial Cancer: A TCGA-based study
Authors: Li He , Li Liwei , Zhao Luyang , Wang Zhiqi 
Published: August 2022

Abstract
Background
Endometrial cancer (EC) identified in pelvic/para-aortic lymph nodes suggests a poorer prognosis. Literature focusing on the role of epithelial-mesenchymal transition (EMT) in lymph node metastasis (LNM) remains few.
Methods
Transcriptional data were acquired from TCGA database. Patients with stage IA-IIIC2 EC were included, constituting the LN positive and negative groups. To evaluate the extent of EMT, an EMT signature composed of 315 genes was adopted. EMT-related genes (ERGs) were obtained from dbEMT2 database, differentially expressed ERGs (DEERGs) of which between the two groups were screened out. On the basis of DEERGs, pathway analysis was carried out. We finally used the logistic regression model to build an ERG-based gene signature with diagnostic value for LNM in EC.
Results
A total of 498 patients were included, with 75 in the LN positive group. Median EMT score of tumor tissues from LN negative group was − 0.369, while that from LN positive group was − 0.296 (P < 0.001), suggesting a more mesenchymal phenotype for LNM cases on the EMT continuum. By comparing the expression profiles, 266 genes were identified as DEGs, in which 184 were upregulated and 82 were downregulated. In pathway analyses, various EMT-related pathways were enriched. DEERGs shared between molecular subtypes were relatively few. The ROC curve and logistic regression analysis screened 7 genes with the best performance to discriminate between the LN positive and negative group, i.e. CIRBP, DDR1, F2RL2, HOXA10, PPARGC1A, SEMA3E and TGFB1. A logistic regression model including the 7-genes based risk score, age, grade, myometrial invasion and histological subtype was built, with an AUC of 0.850 and a good calibration (P = 0.074). In a validation dataset composed of 83 EC patients, the model exhibited a promising diagnostic value and was well calibrated (P = 0.42).
Conclusion
The EMT status and expression of ERGs varied in EC tissues from LN positive and negative cases, involving multiple EMT-related signalling pathways, and the distribution of DEGs differed among molecular subtypes. An ERG-based gene signature including 7 DEERGs exhibited a potential application value.