bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone marrow mesenchymal stem cells inhibit hepatic fibrosis via the AABR07028795.2/rno-miR-667-5p axis
Authors: Feng Yuan , Li Yanjie , Xu Mingxing , Meng Hongyu , Dai Cao , Yao Zhicheng , Lin Nan 
Published: July 2022

Abstract
Background
The mechanism of bone marrow mesenchymal stem cells (BMSCs) in treating hepatic fibrosis remains unclear.

Methods
TGF-β1-induced hepatic stellate cell (HSC)-T6 and CCl4-induced hepatic fibrosis rats were treated with BMSCs. HSC-T6 cell activity was determined using the cell counting kit-8 assay, and the histology change was evaluated using hematoxylin and eosin and Masson staining. The expression of fibrosis markers was determined using real-time quantitative PCR, Western blotting, and immunocytochemistry. RNA sequencing (RNA-seq) was used to screen the lncRNAs involved in the effect of BMSCs in fibrosis, and the function of fibrosis-associated lncRNA in fibrosis histology change and fibrosis marker expression was investigated. The potential miRNA target of lncRNA was predicted using R software. The interaction between lncRNA and miRNA was verified using luciferase report system and RNA immunoprecipitation (RIP) in 293T and HSC-T6 cells.

Results
BMSC attenuated TGF-β1-induced HSC-T6 activation and suppressed the expression of fibrosis-associated gene (MMP2, Collagen I, and αSMA) expression at the transcription and translation levels. BMSC treatment also improves hepatic fibrosis in rats with CCl4-induced fibrosis by decreasing the expression of fibrosis-associated genes and suppressing collagen deposition in the liver. RNA-seq revealed that AABR07028795.2 (lnc-BIHAA1) was downregulated in the TGF-β1-induced HSC-T6 after treatment with BMSCs as compared with those in TGF-β1-induced HSC-T6, and subsequently, functional analysis showed that lnc-BIHAA1 plays a beneficial role in suppressing hepatic fibrosis. Luciferase activity assay and RIP revealed that lnc-BIHAA1 interacted with the miRNA, rno-miR-667-5p, functioning as a fibrosis phenotype suppressor in TGF-β1-induced HSC-T6. Moreover, overexpression of rno-miR-667-5p significantly reverses the effect of lnc-BIHAA1 on HSC-T6.

Conclusions
BMSC treatment suppresses hepatic fibrosis by downregulating the lnc-BIHAA1/rno-miR-667-5p signaling pathway in HSCs. Our results provide a scientific basis for establishing BMSCs as a biological treatment method for liver fibrosis.
Infarct-preconditioning exosomes of umbilical cord mesenchymal stem cells promoted vascular remodeling and neurological recovery after stroke in rats
Authors: Ye Yi-Chao , Chang Zhe-Han , Wang Peng , Wang You-Wei , Liang Jun , Chen Chong , Wang Jing-Jing , Sun Hong-Tao , Wang Yi , Li Xiao-Hong 
Published: July 2022

Abstract
Background
Stroke is the leading cause of disability worldwide, resulting in severe damage to the central nervous system and disrupting neurological functions. There is no effective therapy for promoting neurological recovery. Growing evidence suggests that the composition of exosomes from different microenvironments may benefit stroke. Therefore, it is reasonable to assume that exosomes secreted in response to infarction microenvironment could have further therapeutic effects.

Methods
In our study, cerebral infarct tissue extracts were used to pretreat umbilical cord mesenchymal stem cells (UCMSC). Infarct-preconditioned exosomes were injected into rats via tail vein after middle cerebral artery occlusion (MCAO). The effect of infarct-preconditioned exosomes on the neurological recovery of rats was examined using Tunel assay, 2,3,5-triphenyltetrazolium chloride (TTC) assay, magnetic resonance imaging (MRI) analyses, modified Neurological Severity Score (mNSS), Morris water maze (MWM), and vascular remodeling analysis. Mi-RNA sequencing and functional enrichment analysis were used to validate the signal pathway involved in the effect of infarct-preconditioned exosomes. Human umbilical vein endothelial cells (HUVECs) were co-cultured with the isolated exosomes. Cell Counting Kit-8 (CCK-8) assay, scratch healing, and Western blot analysis were used to detect the biological behavior of HUVECs.

Results
The results showed that compared with normal exosomes, infarct-preconditioned exosomes further promoted vascular remodeling and recovery of neurological function after stroke. The function of upregulated miRNAs and their target genes which is beneficial to vascular smooth muscle cells verified the importance of vascular remodeling in improving stroke. Better resistance to oxygen–glucose deprivation/reoxygenation (OGD/R), reduced apoptosis, and enhanced migration were observed in infarct-preconditioned exosomes-treated umbilical vein endothelial cells.

Conclusions
Our results demonstrated that infarct-preconditioned exosomes promoted neurological recovery after stroke by enhancing vascular endothelial remodeling, suggested that infarct-preconditioned exosomes could be a novel way to alleviate brain damage following a stroke.
The Substance Use Treatment and Recovery Team (START) study: protocol for a multi-site randomized controlled trial evaluating an intervention to improve initiation of medication and linkage to post-discharge care for hospitalized patients with opioid use disorder
Authors: Ober Allison J. , Murray-Krezan Cristina , Page Kimberly , Friedmann Peter D. , Chan Osilla Karen , Ryzewicz Stephen , Huerta Sergio , Mazer Mia W. , Leamon Isabel , Messineo Gabrielle , Watkins Katherine E. , Nuckols Teryl , Danovitch Itai 
Published: July 2022

AbstractBackgroundPeople with opioid use disorder experience high burden of disease from medical comorbidities and are increasingly hospitalized with medical complications. Medications for opioid use disorder are an effective, life-saving treatment, but patients with an opioid use disorder admitted to the hospital seldom initiate medication for their disorder while in the hospital, nor are they linked with outpatient treatment after discharge. The inpatient stay, when patients may be more receptive to improving their health and reducing substance use, offers an opportunity to discuss opioid use disorder and facilitate medication initiation and linkage to treatment after discharge. An addiction-focus consultative team that uses evidence-based tools and resources could address barriers, such as the need for the primary medical team to focus on the primary health problem and lack of time and expertise, that prevent primary medical teams from addressing substance use.MethodsThis study is a pragmatic randomized controlled trial that will evaluate whether a consultative team, called the Substance Use Treatment and Recovery Team (START), increases initiation of any US Food and Drug Administration approved medication for opioid use disorder (buprenorphine, methadone, naltrexone) during the hospital stay and increases linkage to treatment after discharge compared to patients receiving usual care. The study is being conducted at three geographically distinct academic hospitals. Patients are randomly assigned within each hospital to receive the START intervention or usual care. Primary study outcomes are initiation of medication for opioid use disorder in the hospital and linkage to medication or other opioid use disorder treatment after discharge. Outcomes are assessed through participant interviews at baseline and 1 month after discharge and data from hospital and outpatient medical records.DiscussionThe START intervention offers a compelling model to improve care for hospitalized patients with opioid use disorder. The study could also advance translational science by identifying an effective and generalizable approach to treating not only opioid use disorder, but also other substance use disorders and behavioral health conditions.Trial registration:Clinicaltrials.gov: NCT05086796, Registered on 10/21/2021.https://www.clinicaltrials.gov/ct2/results?recrs=ab&cond=&term=NCT05086796&cntry=&state=&city=&dist =
Identifying unique barriers to implementing rural emergency department-based peer services for opioid use disorder through qualitative comparison with urban sites
Authors: Watson Dennis P. , Staton Monte D. , Gastala Nicole 
Published: July 2022

Abstract
Background
In an effort to address the current opioid epidemic, a number of hospitals across the United States have implemented emergency department-based interventions for engaging patients presenting with opioid use disorder. The current study seeks to address gaps in knowledge regarding implementation of a sub-type of such interventions, emergency department-based peer support services, in rural areas by comparing implementation of rural and urban programs that participated in Indiana’s Recovery Coach and Peer Support Initiative (RCPSI).

Methods
We conducted a secondary analysis of qualitative semi-structured implementation interviews collected as part of an evaluation of 10 programs (4 rural and 6 urban) participating in the RCPSI. We conducted interviews with representatives from each program at 3 time points over the course of the first year of implementation. Our deductive coding process was guided by the Consolidated Framework for Implementation Research (CFIR) and an external context taxonomy.

Results
We identified key differences for rural programs corresponding to each of the 5 primary constructs in the coding scheme. (1) Intervention characteristics: rural sites questioned intervention fit with their context, required more adaptations, and encountered unexpected costs. (2) External context: rural sites were not appropriately staffed to meet patient needs, encountered logistical and legal barriers regarding patient privacy, and had limited patient transportation options. (3) Inner setting: rural sites lacked strong mechanisms for internal communication and difficulties integrating with pre-existing culture and climate. (4) Characteristics of individuals: some rural providers resisted working with peers due to pre-existing attitudes and beliefs. (5) Implementation process: rural sites spent more time identifying external partners and abandoned more components of their initial implementation plans.

Conclusions
Findings demonstrate how rural programs faced greater challenges implementing emergency department-based peer services over time. These challenges required flexible adaptations to originally intended plans. Rural programs likely require flexibility to adapt interventions that were developed in urban settings to ensure success considering local contextual constraints that were identified by our analysis.
Erector spinae plane block for postoperative analgesia for above-the-knee amputation: a case report
Authors: Langnas Erica M. , Gray Andrew , Braehler Matthias 
Published: July 2022

Abstract
Background
Above-the-knee amputations (AKA) are common surgeries that frequently use neuraxial or peripheral nerve blocking techniques for both intraoperative and postoperative analgesia. It is not uncommon that patients present with contraindications to neuraxial anesthesia.

Case presentation
We identified a relatively novel use of erector spinae plane block (ESP) for above-the-knee amputation that allows for adequate pain control postoperatively when there are contraindications for neuraxial.

Conclusion
While data on ESP at the thoracic level is well described, less is known about the expected coverage for lumbar ESP. This case suggests that at the level of L3, there is sufficient dermatomal spread for an AKA.
Factors associated with left ventricular diastolic dysfunction in patients with septic shock
Authors: Ge Wei-Dong , Li Feng-Zhi , Hu Bang-Chuan , Wang Li-Hong , Ren Ding-Yuan 
Published: July 2022

Abstract
Purpose
To investigate risk factors associated with left ventricular diastolic dysfunction (LVDD) of patients with septic shock.

Materials and methods
Patients with septic shock concomitant with or without LVDD were retrospectively enrolled and divided into the LVDD group (n = 17) and control without LVDD (n = 85). The clinical and ultrasound data were analyzed.

Results
A significant (P < 0.05) difference existed between the two groups in serum creatinine, APACHE II score, serum glucose, triglyceride, BUN, FT4, LAVI, mitral E, average e’, E/average e’, septal e’, septal e’/septal s’, E/septal e’, lateral s’, lateral e’, and E/lateral e’. LAVI > 37 mL/m2, septal e’ < 7 cm/s (OR 11.04, 95% CI 3.38–36.05), septal e’/septal s’ < 0.8 (OR 4.09, 95% CI 1.37–12.25), E/septal e’ > 15 (OR 22.86, 95% CI 6.09–85.79), lateral e’ < 8 cm/s (OR 9.16, 95% CI 2.70–31.07), E/lateral e’ > 13 (OR 52, 95% CI 11.99- 225.55), lateral s’ < 10 (OR 3.36, 95% CI 1.13–9.99), average e’ > 10, E/average e’ > 10 (OR 9.53, 95% CI 2.49–36.46), APACHE II score > 16 (OR 3.33, 95% CI 1.00–11.03), SOFA > 5 (or 3.43, 95% CI 1.11–10.60), BUN > 12 mmol/L (OR 3.37, 95% CI 1.15–9.87), serum creatinine > 146 μmol/L (OR 5.08, 95% CI 1.69–15.23), serum glucose > 8 mmol/L (OR 3.36, 95% CI 1.09–10.40), and triglyceride > 1.8 mmol/L were significant (P < 0.05) risk factors for LVDD. LAVI > 37 ml/m2, lateral e’ < 8 cm/s, E/lateral e’ > 13, and SOFA > 5 were significant (P < 0.05) independent risk factors for LVDD. ROC curve analysis demonstrated that the cut-off value and AUC were 37.09 mL/m2 and 0.85 for LAVI, 8.00 cm/s and 0.89 for lateral e’, 12.86 and 0.82 for E/lateral e’, and 5.00 and 0.69 for SOFA, respectively.

Conclusion
Left atrial volume index, mitral lateral e’, E/lateral e’, and SOFA score are significant independent risk factors for predicting left ventricular diastolic dysfunction in patients with septic shock.
Evaluation of the Progressive Collapse of the Reinforced Concrete Frames Considering the Soil–Structure Interaction: Parametric Study Based on the Sensitivity Index
Authors: Kakhki Seyed Ali Ekrami , Kheyroddin Ali , Mortezaei Alireza 
Published: July 2022

AbstractIn this essay, to investigate the progressive collapse of the reinforced concrete (RC) frames, a nonlinear static pushdown analysis was performed with column removal scenarios from the first story. At first, a numerical model was simulated and verified with the experimental model in SeismoStruct software without soil–structure interaction (SSI). Afterward, the foundation, soil, and the RC frame were modeled simultaneously in FLAC software and verified with the numerical model of the SeismoStruct software. Furthermore, the effect of SSI was studied on the progressive collapse of RC frames based on the sensitivity index (SI). The sensitivity index is defined as the ratio of the residual capacity under gravity loading of the structure by removing the column to the value of the undamaged structure. The results showed that by considering SSI, the sensitivity index decreases. Then, a parametric study of the framed structures (thickness of the foundation) and substructures (soil density, soil types, soil layers, and the soil saturation conditions) was performed to evaluate the progressive collapse-resisting capacity based on the sensitivity index. The results showed that by considering SSI, with an increase in the soil density and decrease in the groundwater level, the conditions would be better for preventing progressive collapse. It was also shown that rock and silty sands (SM), compared to other studied soil types, and SM and silty sands—silty clay with low plasticity—silty sands (SM-CL/ML-SM), compared to other studied soil layers, are better for preventing progressive collapse.
Spatial market integration of rice in Bangladesh in the presence of transaction cost
Authors: Alam Mohammad Jahangir , McKenzie Andrew M. , Begum Ismat Ara , Buysse Jeroen , Wailes Eric J. , Sarkar Md. Abdur Rouf , Al Mamun Abdullah , Van Huylenbroeck Guido 
Published: July 2022

AbstractModeling transaction costs is of particular importance when analyzing price integration in developing countries. Spatial price integration among five major Bangladesh rice markets are examined over the post-liberalization period that covered a long time span from January 1999 to December 2021, the longest period covered ever. We account for nonlinear price adjustments induced by transaction costs using a threshold cointegration approach to account for the impact of transaction costs in the price adjustment process. Our results show that large price deviations from long-run equilibrium are corrected within 2–3 months. Our results provide strong supporting evidence of the presence of threshold effects, which means that large transaction costs contribute to slow equilibrium price adjustments. Thus, our results provide important policy implications for Bangladesh rice markets, namely that polices aimed at reducing transaction costs are needed to engender greater pricing efficiency in Bangladesh rice markets.
Development of a management protocol for internal carotid artery injury during endoscopic surgery: A modified Delphi method and single-center multidisciplinary working group
Authors: Hamour Amr F. , Laliberte Frederick , Padhye Vikram , Monteiro Eric , Agid Ronit , Lee John M. , Witterick Ian J. , Vescan Allan D. 
Published: July 2022

Background Intra-operative internal carotid artery (ICA) injury during transnasal endoscopic surgery is a potentially catastrophic event. Such an injury is life-threatening in the immediate setting, with a reported peri-operative mortality rate of 10%. Nasal packing, muscle patches, direct vessel closure, and endovascular techniques have been described as useful strategies for managing ICA bleeds. The objective of this study was to develop a formalized management protocol for intra-operative ICA injury through engagement with a multi-disciplinary panel. Methods A modified Delphi method including literature review, iterative rounds of stakeholder feedback, and expert panel discussions was used to develop a management protocol for ICA injury during transnasal endoscopic surgery. The 10-person multi-disciplinary panel included otolaryngologists, neurosurgeons, interventional neuroradiologists, anesthesiologists, and operating room nursing staff. Results After three rounds of stakeholder engagement with the expert panel, consensus was reached on important elements to include within the protocol. The protocol was divided in three categories: Alert, Control, and Transfer. ‘Alert’ focusses on early communication with anesthesia and nursing staff. ‘Control’ focusses on techniques to expose the injury and obtain hemostasis or adequate tamponade. Lastly, ‘Transfer’ describes the process of contacting neuro-interventional radiology and safely transferring the patient. A one-page handout of the protocol was developed for placement in operating theatres. Conclusion Due to the life-threatening nature of ICA injury, it is imperative that endoscopic sinus and skull base surgeons are prepared to manage this complication. Using a modified Delphi method with a multidisciplinary expert panel, a protocol for management of intra-operative ICA injury was developed. Graphical Abstract
Russian speakers’ media engagement and acculturation in Finland and Latvia
Authors: Khalimzoda Ilkhom , Siitonen Marko 
Published: July 2022

AbstractThis comparative study looks into Russian speakers’ acculturation in Finland and Latvia by contrasting their cultural involvement and cultural preference Carlson and Güler (J Int Migr Integr 19:625–647, 2018. 10.1007/s12134-018-0554-4) with their self-reported news media use. Drawing on survey data collected from both countries (N = 224), the findings show that participants in both countries scored closer to biculturalism than monoculturalism. Majority of the respondents report predominantly engaging with non-Russian news media sources. However, regardless of the societal context, respondents who were more engaged with Russian news media sources also scored higher on cultural preference (towards Russian culture of origin). Further significant differences and implications are discussed.
Current distribution and disease association of Ixodidae (hard ticks) in Nigeria
Authors: Kyari Stephen , Ogwiji Matthew , Igah Olanrewaju Eyitayo , Orakpogheno Ochuko , Gasaliyu Khadijat Abidemi 
Published: July 2022

Abstract
Background
This review documents the hard tick species that have been reported in Nigeria, their distribution, pathogens transmitted and disease they cause in Nigeria; the literature review considered studies that were conducted between 2000 and 2021.

Main body
The hard tick (Ixodidae) species widely present in the country are those of the genera; Amblyomma, Rhipicephalus/Boophilus, Hyalomma, Ixodes, Dermacentor and Haemaphysalis among which, Amblyomma sp, Hyalomma sp and Rhipicephalus sp. were most widely reported. Ticks cause serious economic losses to the livestock industry via their negative impact on animal health in general and possible transmission of zoonotic pathogens by some species. Ticks’ distribution is favoured by factors which include uncontrolled cross-border movement of animals, lack of strict quarantine measures, extensive animal grazing and suitable climatic condition. Tick-borne diseases commonly reported included Babesiosis, Anaplasmosis, Theileriosis and Ehrlichiosis, while diseases such as Crimean-Congo Haemorrhagic Fever and Africa tick bite fever are under-reported. Also, there is paucity of information on the economic impact of these diseases. Measures that have been implemented in the control of ticks included the use of acaricides and to some extent vaccines.

Conclusions
Ticks and tick-borne diseases constitute serious economic important and studies to fully ascertain these at the national level is recommended. Also, there is need for identification of other species of ticks and under-reported tick-borne diseases to develop more integrated control measures.
Building urban resilience through sustainability-oriented small- and medium-sized enterprises
Authors: Burch Sarah , DiBella Jose , Wiek Arnim , Schaltegger Stefan , Stubbs Wendy , Farrelly Megan , Ness Barry , McCormick Kes 
Published: July 2022

AbstractThe unfolding COVID-19 pandemic, and the unprecedented social and economic costs it has inflicted, provide an important opportunity to scrutinize the interplay between the resilience of small and medium-sized enterprises (SMEs) and the resilience of the communities they are embedded in. In this article, we articulate the specific ways that SMEs play a crucial, and underappreciated role in building resilience to human and natural hazards, and provide new opportunities to accelerate the adoption of sustainability practices through the configuration of ‘enabling ecosystems’ geared towards promoting sustainability in the private sector. We argue that capacity-building and experimentation are not only required within companies, but also throughout this emerging supportive ecosystem of policies, resources (i.e. finance, materials, skills), governance actors, and intermediaries to adequately focus investment, technical capabilities and innovation. Ultimately, we call for a new transdisciplinary action research agenda that centers on SMEs as pivotal actors and amplifiers of community resilience; while recognizing that these firms are themselves in need of support to secure their own capacity to respond to, and transform in light of, crises. This research program calls for recognizing and applying the lessons that the pandemic presents to the urgent need for accelerated climate action. This will be enabled by developing more targeted approaches to collaborative capacity-building activities in SMEs that feed into experimentation and allow for the accelerated adoption of deliberate and strategic resilient business practices and models.