bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of bacterial diversity associated with assisted reproductive technologies through next-generation sequencing
Authors: Vajpeyee Manisha , Tiwari Shivam , Yadav Lokendra Bahadur , Tank Parikshit 
Published: September 2022

Abstract
Background
To assess bacterial diversity in infertile couples with their biochemical pregnancy outcomes. Using a retrospective case-control study design, participants were recruited for collection of vaginal swab, follicular fluid, endometrial fluid, and semen samples. The microbial composition was analyzed by 16S ribosomal RNA (rRNA) gene amplification with (MinION) Oxford Nanopore Ltd.

Results
Our findings revealed that age and endometrial thickness had a significant impact on the pregnancy success rate of pregnant (P) and non-pregnant (NP) patients receiving IVF, with high levels of luteinizing hormone, estrogen, and progesterone in the P group. In addition, the partial least squares discriminant analysis (PLS-DA) revealed a difference in microbial composition between the P and NP groups, as well as a higher microbial abundance in non-pregnant patients compared to pregnant patients. After comparison between pregnant patients and non-pregnant patients, pregnant patients had a higher abundance of Firmicutes and Proteobacteria and a lower abundance of Actinobacteria, Fusobacterium, and Bacteroidetes at the phylum level. Non-pregnant patients had a lower abundance of the probiotics lactobacillus and a higher abundance of infections Prevotella and Gardnerella at the genus level. As a result, a disordered microbiota in non-pregnant patients, characterized by a decrease in probiotics and an increase in pathogens, could be used as a possible marker for a higher IVF failure rate.

Conclusion
Alteration of the microbiota of the reproductive tract or the presence of certain microbes, regardless of the degree of pathogenicity that can affect fertilization, as well as implantation and subsequent embryonic development. This could result in failed fertility treatments and a lower live birth (LBR) rate.
Genistein anticancer efficacy during induced oral squamous cell carcinoma: an experimental study
Authors: Hussein Ahmed M. , Attaai Abdelraheim H. , Zahran Asmaa M. 
Published: September 2022

AbstractBackgroundAbout 7 million people die from various types of cancer every year representing nearly 12.5% of deaths worldwide. This fact raises the demand to develop new, effective anticancer, onco-suppressive, and chemoprotective agents for the future fighting of cancers. Genistein exhibits pleiotropic functions in cancer, metabolism, and inflammation. It functions as an antineoplastic agent through its effect on the cell cycle, apoptotic processes, angiogenesis, invasion, and metastasis.Aim of the studyThe current study aimed to study the genistein onco-suppressive effects during 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral carcinogenesis in hamsters’ buccal pouch utilizing flow cytometry analysis (FMA), as a fast-diagnosing tool, in addition to the histopathology.Material and methodsThe buccal mucosa of adult male Syrian hamsters was painted with paraffin oil only (group 1), DMBA mixed in mineral oil (group 2), or orally administrated genistein along with painting DMBA (group 2B). The buccal mucosa was utilized for flow cytometric analysis and histopathological examination.ResultsGrossly, DMBA-induced carcinogenesis started at the 9th week. Progressive signs appeared in the following weeks reaching to large ulcerative oral masses and exophytic nodules at the 21st week. Histologically, invasive well-differentiated oral squamous cell carcinoma (OSCC) appeared in the underlying tissues from the 12th week, showing malignant criteria. Genistein had delayed clinicopathological change, which started 6 weeks later, than the DMBA-painted hamsters, as mild epithelial dysplastic changes. This became moderate during the last 6 weeks, without dysplastic changes. Flow cytometry revealed that DMBA led to considerable variation in DNA proliferation activity, aneuploid DNA pattern, in 47.22% of hamsters and significantly raised the S-phase fragment (SPF) values, which drastically reduced after genistein treatment.ConclusionTaken together, genistein could be employed as an onco-suppressive agent for carcinogenesis. Moreover, FMA could be used as an aiding fast tool for diagnosis of cancer.
Value and imaging findings of the magnetic resonance in the posterolateral corner complex injuries of the knee
Authors: Algizawy Ahmed Abd El-Wahab Mahmoud , Sakr Hossam Moussa , Nassif Mohamed Amin , Bassiouny Ahmed Mohamed 
Published: September 2022

Abstract
Background
MRI is considered the key tool for evaluation of knee injuries, notably the posterolateral corner, due to its complexity and multi-ligament involvement. This prospective study was conducted from September 2020 to September 2021 aiming to clarify the value and possible MRI findings in assessing the posterolateral corner (PLC) knee injuries which can subsequently result in an improvement in the management of these cases, preventing devastating consequences.

Results
The current study was conducted on 30 patients (11 females and 19 males) whose mean age was 42 years. Imaging by a closed magnet (1.5 T) MRI was applied to 30 patients with traumatic history to the knee using: axial, sagittal, coronal T1WI, T2WI, and proton density fat sat sequences. The lateral collateral ligament (LCL) was the most commonly affected ligament. Grade I injuries were the most common form of injuries. Twenty-four (80%) patients suffered from LCL injury, popliteal complex injuries were in 21 (70%) patients, 10 (33.3%) patients had biceps femoris tendon injury, while injury to the popliteofibular ligament was seen in 8 (26%) patients. The arcuate ligament was poorly defined in 6 (20%) patients, indicating that it had torn, lateral head of gastrocnemius injury was seen in 5 (16.7%) patients and 4 (13.3%) patients showed injury to the iliotibial band. Correlation with arthroscopic findings was considered the gold standard of the results whenever available. That was applied to 16 cases who had clinically significant knee injuries and arthroscopy was requested.

Conclusions
MRI is a key tool for determining a correct pre-surgical evaluation and diagnosis. It is critical to have a thorough understanding of the radiological PLC anatomy, as well as the presence of various pathologies on MRI. It allows the determination of whether the tendons and ligaments are partially or completely torn, besides any related injuries preventing possible poor consequences after the restoration of the cruciate ligaments.
Integrated biofertilization using yeast with cyanobacteria on growth and productivity of wheat
Authors: Hamed Seham M. , El-Gaml Naayem Mohamed , Eissa Sherif Thabet 
Published: September 2022

Abstract
Background
The heavy use of conventional mineral fertilization considerably increased wheat growth and yield components. However, the excessive nitrogen fertilization accounts for large expenses on farmers’ budget and has negative environmental drawbacks to soil and water ecosystems. Recently, establishing wheat plants associations with a variety of N2-fixing cyanobacteria and/or growth promoting microorganisms in farming systems as nutrients source is seen as ecofriendly and economically feasible solution.

Results
In this work, the influence of different nitrogen (N) levels (100% N, 75% N, and 50% N as urea) and cyanobacteria (Cy) and/or yeast (Y) inoculation each alone or both in combination with different nitrogen levels were assessed on two different wheat (Triticumae stivum L.) genotypes (Sids-14 and Giza-171) through field experiments over two consecutive seasons (2019/2020 and 2020/2021). Although, the full recommended dose (FRD) (100% N) showed the highest chlorophyll (Chl) a, b content during tillering stage, grains quality (NPK-uptake, protein%), and wheat yield components in both genotypes. The integrated inoculation of cyanobacteria and yeast with 50% N, 75% N-fertilization enhanced wheat growth characteristic (Chl a,b) and upgraded soil microbial community (nitrogenase activity and CO2 evolution) in Sids-14 and Giza-171. Cy combined with Y and partial N-fertilization levels enhanced NPK-uptake Kg/fed and protein% in wheat grains in the two wheat genotypes. Moreover, this combination recorded a wheat yield components (plant height, number of spikes/m2, number of grains/spike, 1000 grains wt (gm) and grains yield (tonne/fed) insignificantly different from those obtained by FRD in wheat cultivation.

Conclusion
The integrated application of cyanobacteria with yeast and 50% N or 75% N-fertilization improved soil fertility index and promoted NPK- uptake, protein% and wheat yield components, showing comparable values to conventional chemical fertilization. Cyanobacteria combined with yeast inoculation had socio-economic benefits as it can save about 25–50% of the required mineral nitrogen fertilizers for wheat crop production.
Design of passive suspension system to mimic fuzzy logic control active suspension system
Authors: Nagarkar Mahesh , Bhalerao Yogesh , Bhaskar Dhiraj , Thakur Ajaykumar , Hase Vaibhav , Zaware Rahul 
Published: September 2022

Abstract
Background
This paper proposes a method for designing a passive suspension system that determines the optimal suspension settings while offering feasible performance near an active suspension system. A mathematical model of a nonlinear quarter car is developed and simulated for control and optimization in MATLAB/Simulink® environment. The input road condition is a Class C Road, and the vehicle moves at 80 kmph. Fuzzy logic control (FLC) action is used to accomplish active suspension system control. An approach for investigating optimal suspension settings based on the FLC control force is described here. The optimized passive suspension system is supposed to have the same suspension travel and velocity as an active suspension system. The least square technique is implemented to optimize the suspension parameters of the passive suspension system.

Results
The initial passive suspension system, FLC active system, and optimized suspension system are simulated in MATLAB/Simulink® environment. It is observed that RMS acceleration for the FLC system is 0.5057 m/s2, which is reduced by 46% (passive suspension system has RMS acceleration of 0.9322 m/s2, which is uncomfortable). For optimized system, RMS acceleration is 0.6990 m/s2. It is observed that the optimized passive suspension system almost mimics the initial FLC active suspension system. For the optimized system, sprung mass acceleration and VDV are improved by 30% and 27%, respectively, compared to the initial passive system.

Conclusion
It is observed that the optimized passive suspension system mimics the initial FLC system. Also, an optimized FLC system has improved health criterion-based results compared to other suspension systems.
Development of gold nanoparticle-based biosensors for COVID-19 diagnosis
Authors: Khan Johra , Rasmi Yousef , Kırboğa Kevser Kübra , Ali Ahmad , Rudrapal Mithun , Patekar Rohan R. 
Published: September 2022

Abstract
Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative organism of coronavirus disease 2019 (COVID-19) which poses a significant threat to public health worldwide. Though there are certain recommended drugs that can cure COVID-19, their therapeutic efficacy is limited. Therefore, the early and rapid detection without compromising the test accuracy is necessary in order to provide an appropriate treatment for the disease suppression.

Main body
Nanoparticles (NPs) can closely mimic the virus and interact strongly with its proteins due to their morphological similarities. NPs have been widely applied in a variety of medical applications, including biosensing, drug delivery, antimicrobial treatment, and imaging. Recently, NPs-based biosensors have attracted great interest for their biological activities and specific sensing properties, which allows the detection of analytes such as nucleic acids (DNA or RNA), aptamers, and proteins in clinical samples. Further, the advances of nanotechnologies have enabled the development of miniaturized detection systems for point-of-care biosensors, a new strategy for detecting human viral diseases. Among the various NPs, the specific physicochemical properties of gold NPs (AuNPs) are being widely used in the field of clinical diagnostics. As a result, several AuNP-based colorimetric detection methods have been developed.

Short conclusion
The purpose of this review is to provide an overview of the development of AuNPs-based biosensors by virtue of its powerful characteristics as a signal amplifier or enhancer that target pathogenic RNA viruses that provide a reliable and effective strategy for detecting of the existing or newly emerging SARS-CoV-2.
Common and uncommon audio-vestibular findings in COVID-19 patients
Authors: Nada Ebtessam Hamed , El-Gharib Amani Mohamed , Mandour Mahmoud 
Published: September 2022

AbstractBackgroundCOVID-19 is the new version of the old coronavirus known since 1960, which caused the Middle East respiratory syndrome (MERS-CoV) in 2012 and the severe acute respiratory syndrome (SARS) in 2003.Symptoms included fever and cough, diarrhea and vomiting, and neurological symptoms like anosmia.MethodsOne hundred twenty-eight patients diagnosed as COVID-19 with audio-vestibular complaints were subjected to audio-vestibular assessment and were included in the study.ResultsIn our study on COVID-19 patients who reported audio-vestibular complaints, hearing loss was found in 43.8% of patients in comparison to vertigo that represented 40.6% of cases. The most common type was sensorineural hearing loss representing 29.7% of patients and which was unilateral and sudden in 35.7% of them. Less commonly conductive hearing loss (CHL) was found in 14.1% of cases the most common form was bilateral mild to moderate CHL (83.3%) due to bilateral middle ear effusion.Among cases with vertigo, the most common etiology was benign paroxysmal positional vertigo (BPPV) (42.5%) then uncompensated vestibular neuritis (VN) (31.5%), and lastly, combined BPPV with VN (25%) of cases. Less frequently we found tinnitus in (13.3%) which was bilateral in (64.7%), labyrinthitis (5.5%), and acute VN 5.5%).The significant increase in the number of audiovestibular complaining cases that were observed in the course of the recurrent waves’ peaks pushed us to study the relationship between the pandemic and the audiovestibular system. The effect of COVID on AV systems is well noticed and management would be mandatory.
Corrections to the thermodynamic quantities of Bose system by the generalized uncertainty principle
Authors: Li Jun-Xian , Zhang Jing-Yi 
Published: September 2022

Abstract
This paper investigated the Bose system in a spherical shell close to the black hole horizon. Several thermodynamic quantities of the Bose system are derived, which are different from those in the flat spacetime, by introducing the generalized uncertainty principle (GUP) into the grand partition function of statistical mechanics. The internal energy and the pressure of the Bose system appear to have a correction term of T
6, while the entropy has a T
5 correction term where both the coefficients are functions of the spacetime component g
00 and the brick wall model parameter ϵ. Taking the Schwarzschild black hole as an example, the physical quantities of the shell such as temperature, pressure and entropy are calculated for the final stage of black hole radiation.
Geometrodynamical description of two-dimensional electrodynamics
Authors: Sobreiro Rodrigo F. 
Published: September 2022

Abstract
Two-dimensional pure electrodynamics is mapped into two-dimensional gravity in the first-order formalism at classical and quantum levels. Due to the fact that the degrees of freedom of these two theories do not match, we are enforced to introduce extra fields from the beginning. These fields are introduced through a BRST exact boundary term, so they are harmless to the physical content of the theory. The map between electromagnetism and gravity fields generate a non-trivial Jacobian, which brings extra features (but also harmless to the physical content of the gravity theory) at quantum level.
Weight Loss, Lifestyle Intervention, and Metformin Affect Longitudinal Relationship of Insulin Secretion and Sensitivity
Authors: Vazquez Arreola Elsa , Knowler William C , Hanson Robert L 
Published: September 2022

Abstract

Context
Insulin secretion and sensitivity regulate glycemia, with inadequately compensated deficiencies leading to diabetes.

Objective
We investigated effects of weight loss, an intensive lifestyle intervention (ILS), and metformin on the relationship between insulin secretion and sensitivity using repository data from 2931 participants in the Diabetes Prevention Program clinical trial in adults at high risk of developing type 2 diabetes.

Methods
Insulin secretion and sensitivity were estimated from insulin and glucose concentrations in fasting and 30-minute postload serum samples at baseline and 1, 2, and 3 years after randomization, during the active intervention phase. The nonlinear relationship of secretion and sensitivity was evaluated by standardized major axis regression to account for variability in both variables. Insulin secretory demand and compensatory insulin secretion were characterized by distances along and away from the regression line, respectively.

Results
ILS and metformin decreased secretory demand while increasing compensatory insulin secretion, with greater effects of ILS. Improvements were directly related to weight loss; decreased weight significantly reduced secretory demand (b=−0.144 SD; 95% CI (−0.162, −0.125)/5 kg loss) and increased compensatory insulin secretion (b = 0.287 SD, 95% CI (0.261, 0.314)/5 kg loss). In time-dependent hazard models, increasing compensatory insulin secretion (hazard ratio [HR] = 0.166 per baseline SD, 95% CI 0.133, 0.206) and weight loss (HR = 0.710 per 5 kg loss, 95% CI 0.613, 0.819) predicted lower diabetes risk.

Conclusion
Diabetes risk reduction was directly related to the amount of weight loss, an effect mediated by lowered insulin secretory demand (due to increased insulin sensitivity) coupled with improved compensatory insulin secretion.
Association of Systemic Trimethyllysine With Heart Failure With Preserved Ejection Fraction and Cardiovascular Events
Authors: Wei Haoran , Zhao Mingming , Wu Junfang , Li Chenze , Huang Man , Gao Jianing , Zhang Qi , Ji Liang , Wang Yan , Zhao Chunxia , Dong Erdan , Zheng Lemin , Wang Dao Wen 
Published: September 2022

Abstract

Context
Carnitine has been associated with cardiac energy metabolism and heart failure, but the association between its precursors—trimethyllysine (TML) and γ-butyrobetaine (GBB)—and heart failure with preserved ejection fraction (HFpEF) remains unclear.

Objective
To evaluate the relationship between TML-related metabolites and HFpEF in an Asian population.

Methods
The cross-sectional component of this study examined the association between plasma TML-related metabolites and HFpEF, while a prospective cohort design was applied to examine the association with incident cardiovascular events in HFpEF. Included in the study were 1000 individuals who did not have heart failure (non-HF) and 1413 patients with HFpEF. Liquid chromatography mass spectrometry was used to assess plasma carnitine, GBB, TML and trimethylamine-N-oxide (TMAO) concentrations.

Results
Plasma GBB and TML were both elevated in patients with HFpEF. After adjusting for traditional risk factors and renal function, TML, but not GBB, was significantly associated with HFpEF. The odds ratio (OR) for the fourth vs first quartile of TML was 1.57 (95% CI 1.09-2.27; P-trend &lt; .01). The OR for each SD increment of log-TML was 1.26 (95% CI 1.08-1.47). Plasma TMAO (P-interaction = 0.024) and estimated glomerular filtration rate (P-interaction = 0.024) modified the TML–HFpEF association. The addition of TML improved the diagnostic value under the multivariable model. In the prospective study of patients with HFpEF, higher plasma TML was associated with increased risk of cardiovascular events.

Conclusion
Plasma TML concentrations are positively associated with HFpEF, and higher plasma TML indicates increased risk of cardiovascular events.
Prospective Analysis of Muscle Adiposity in Children With X-linked Hypophosphatemic Rickets vs Control Children
Authors: Nguyen-Khac Virginie , Bonnet-Lebrun Aurore , Linglart Agnès , de Tienda Marine , Berkenou Jugurtha , Mannes Inès , Adamsbaum Catherine , Wicart Philippe , Skalli Wafa 
Published: September 2022

Abstract

Context
Children with X-linked hypophosphatemic (XLH) rickets have muscle weakness that severely impairs their function. Intermuscular and intramuscular adipose tissue (IMAT and intraMAT, respectively) may contribute to this muscle weakness.

Objective
This work aimed to compare IMAT and intraMAT in XLH children vs typically developing (TD) children.

Methods
A prospective, monocentric cohort study was conducted of XLH (n = 11; aged 10.3 years [6-17]) and TD children (n = 22; aged 10.2 years [5-15.5]). All children underwent magnetic resonance imaging of the lower limbs; IMAT and intraMAT percentages were calculated after manual contouring of each muscle of the thigh and the deep fascia at mid-thigh level.

Results
XLH children were comparable in age but shorter and heavier than TD children (P = .001 and P = .03, respectively). They had smaller muscle length and volume than TD children (P &lt; .001) but there was no statistically significant difference in muscle cross-sectional area between the groups (P = .833). The total percentage of IMAT was higher in XLH children (8.66% vs 3.60% in TD children; P &lt; .0001). In addition, though the total percentage of intraMAT did not differ significantly (12.58% and 10.85% in XLH and TD children, respectively; P = .143) intraMAT was statistically significantly higher in XLH children than TD children in 4 of the 13 muscles studied.

Conclusion
Our results show that IMAT is higher in young children with XLH, independently of obesity and overweight. Further, these results will facilitate both the early prevention of functional and metabolic consequences of the increase in adipose tissue in XLH children.