bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term dynamic of the jumbo squid geographic distribution in Humboldt Current System
Authors: Ibanez Christian M. 
Published: September 2022

Abstract
Many cephalopods are plastic in their response to environmental variability; factors such as temperature and salinity are considered to have an important role in their abundance and distribution. The jumbo squid (Dosidicus gigas) is an important ecosystem component and fishery resource of the Humboldt Current System (HCS) that shows extreme changes in its distribution and abundance. The present study reveals changes of its spatial distribution using an occurrence index built using a 57-years dataset from fishery landings. Although the index showed temporal changes, it showed no relation with temperature and its anomalies in HCS. It is necessary to consider other variability sources both intrinsic and external to understand the changes in the jumbo squid distribution. This approach could be useful to explore long-term changes in geographical distribution of marine species using catch data.
Effects of transcranial direct current stimulation (tDCS) in subjects with severe traumatic brain injury in the subacute phase: Study protocol for a triple-blind randomized controlled clinical trial.
Authors: Cordeiro Barbara Naeme de Lima , Kuster Elizângela , Thibaut Aurore , Nascimento Lucas Rodrigues , Gonçalves Jessica Vaz , Arêas Guilherme Peixoto Tinoco , Paiva Wellingson Silva , Arêas Fernando Zanela da Silva 
Published: September 2022

Abstract
Background: Transcranial direct current stimulation (tDCS) is a noninvasive brain stimulation technique tested in several diseases, including traumatic brain injury (TBI). Anodic and cathodic tDCS are typically used to increase and decrease excitability, respectively, and depending on mounting and stimulation parameters, tDCS can target different brain networks. This study will examine the immediate and late effects of tDCS on cognitive function, pain, functional independence, anxiety and depression, in-hospital complications and length of stay in subjects with severe TBI in the subacute phase.
Methods: This is a randomized, two-arm, prospectively enrolled study with blinded allocation, raters, participants, and therapists blind, and intention-to-treat analysis. The study will consist of 30 subjects with severe TBI, who were discharged from the intensive care unit at least two weeks ago. Participants will be randomly allocated into an experimental group (i.e, tDCS 2mA active for 20 minutes) or a control group (i.e, tDCS sham for 20 minutes). Participants will receive sessions tDCS 5 times a week for 4 weeks, for a total of 20 sessions. Outcome measures will be collected by trained researchers at baseline (week 0), after 20 tDCS sessions, i.e at week 4 (week 4), 6 months later by telephone, and one year after treatment, also by phone.
Discussion: The search for adjuvant therapies to improve TBI outcomes is challenging, mainly due to the heterogeneity, complexity and severity of the lesions. Thus, the results of this trial may result in an important advance for neurological rehabilitation.
Trial registration: Brazilian Registry of Clinical Trials (ReBEC), RBR-37t4kc4. Registered prospectively on July 26rd , 2021
An Efficient Lexicographic Approach to Solve Multi-Objective Multi-Port Fabric Dyeing Machine Planning Problem
Authors: Demir Yunus 
Published: September 2022

Abstract

In this study, a decision problem for the planning of the fabric dyeing process of a factory that produces towels is discussed. Towel fabric bolts in different quantities and features belonging to different orders are dyed by batching under certain restrictions in multi-port dyeing machines. In this problem, three different objectives are aimed to be optimized: Total tardiness, total number of washes (number of sequence dependent setups), total machine fixed cost. Since the prioritization for the objectives is made clearly by the company officials, the lexicographic approach has been preferred as the solution. First, the integer programming model of the problem is created. Lexicographic optimization approach was applied for the solution of the model. Afterwards, an effective Lexicographic multi-objective Genetic Algorithm, which is an extension of the single-objective Genetic Algorithm, is proposed. The performance of both solution methods was tested with randomly generated problems. Comparative results showed that the proposed lexicographic multi-objective genetic algorithm gave effective results.
Adverse effects of montelukast in children
Authors: Al-Shamrani Abdullah saeed , Alharbi Saleh , Kobeisy Sumayyah , Alkhater Suzan , Alalkami Haleimah , Alahmadi Turki , Almutairi Aisha , Alharbi Adel , Yousef Abdullah 
Published: September 2022

Abstract
Montelukast is a leukotriene receptor antagonist (LTRA) commonly prescribed for asthma, allergic rhinitis and sleep-related breathing disorders. Recently, some studies have reported several adverse events, such as neuropsychiatric disorders and sleep disturbances, among children.
Objective:
To obtain more insight into the safety profile of montelukast for children with asthma, allergic rhinitis and sleep-related breathing disorders.
Method and results:
We retrospectively studied all adverse drug reactions to montelukast among 385 children 6 months or older in five tertiary centers over a two-year period. A total of 89.6% were asthmatic, 50% had allergic rhinitis and 13.6% had sleep-related breathing disorders; Singulair was the most common type of montelukast used (67.9%). This study reported a high prevalence of adverse effects among 123 patients (31.9%), predominantly in those aged 4-9 years (52.8%), followed by adolescent children (24.4%) and toddlers (22.8%). Two adverse effects were reported in 9.8% of the children, while three or more were reported in 5.5%. Sleep disturbance was the most commonadverse effect, affecting 15.1% of participants (overlap was common; 5.5% of children experienced sleep difficulties, 4.4% experienced sleep interruption and decreased sleep, and 1.82% experienced nightmares), followed by agitation (10.4%), pain (9.4%) and hyperactivity (6.8%). No serious adverse effects were reported. Eleven percent of families faced difficulties in purchasing montelukast, and only 57% of families had insurance. Misconceptions were common (9.8% reported it to be a steroid, while 30.6% believed it to be a bronchodilator). Although 81% of the families believed it was an effective and preventive medication, 5.3% stopped the drug due to concern about side effects, especially agitation (3%) and nightmares (0.6%).
Conclusion:
These data demonstrate that montelukast is effective, but the associated adverse neuropsychiatric drug reactions are more prevalent than those reported in the literature. In particular, sleep disturbance, agitation, pain and hyperactivity were observed. Pediatricians should be aware of such adverse effects. Misconceptions about montelukast are still common, and parental counseling and urgent epidemiological studies are needed to quantify the risk for management plans.
Rotator cuff tears are associated with significant psychiatric problems
Authors: KARAASLAN Fatih , AYDOĞAN Serhat , Yurdakul Emre , Yalvaç Mehmet , Dülgeroğlu Turan Cihan , Karaaslan Özgül 
Published: September 2022

AbstractQuestions/Purpose: In this prospective study of patients who underwent rotator cuff (RC) repair, we aimed to evaluate the pre- and post-operative rates of depression, anxiety, and sleep disturbances, investigate the impact of psychological health and sleep disturbances on the surgical outcomes, and identify the preoperative predictors for surgical outcomes.Methods: Patients who underwent RC repair after failed non-operative treatment were included in this study. All patients were evaluated using the visual analogue scale, American Shoulder and Elbow Surgeons Shoulder Score, constant score, the abbreviated World Health Organization Quality of Life questionnaire, Beck depression inventory, Beck anxiety inventory, and the Pittsburgh sleep quality index preoperatively and postoperatively at the 6-month follow up.Results: Eighty-two patients (20 males and 62 females) with a mean age of 67 years were included in this study. Depression was observed in 48% (mean score = 10.83), anxiety in 51% (mean score = 10.11), and sleep disturbance in 69% (mean score = 6.00) of patients pre-operatively.Conclusions:Shoulder function, depression, and anxiety scores significantly improved, while sleep disturbance decreased, after RC repair. RC tears are associated with significant psychiatric problems.
Evaluation of flood period effects on nutrient and gross primary productivity changes in Shadegan wetland
Authors: Azizi Fatemeh , savari ahmad , Amini Faedeh , asgari hossein mohammad , doustshenas babak 
Published: September 2022

Abstract
Floodplain wetlands in semi-arid regions that are subject to changes in hydrology and geomorphology during the annual flood period have measurable impacts of these ecosystems. This study aimed to investigate the effects of water level fluctuations during the flood period on the water physicochemical properties, nutrient status, sestonic chlorophyll a, and composition of the phytoplankton community of phytoplankton in Shadegan ‎wetland. The hypothesis of this study which was based on water level fluctuations during the flood period ‎is the key controlling regulator of the wetland’s physicochemical, nutrient status, phytoplankton community structure driven by its influence on the wetland’s hydrological connectivity in combination with other environmental parameters. The results showed that most environmental variables and nutrients differed significantly each season in the three different periods of ‎flood ‎for three consecutive years (2017–2019) in the Shadegan wetland. During flood season, the nutrient concentration, carbon content of the water body, and chlorophyll a conentration are significantly higher than during dry and wet seasons. There was a significant difference in the structural community of phytoplankton in flood periods. The results suggested that changes in water levels during flood periods in Shadegan wetland can directly affect the phytoplankton ‎community and gross primary productivity or indirectly via changes in nutrient levels, hydrological ‎connectivity, and other environmental parameters. This study can present beneficial knowledge of hydrological and ecological dynamics in wetland, providing a useful reference in the management of irregular withdrawals from water resources, and increasing the cultivation lands on upstream rivers of the Shadegan Wetland.
Matrix stiffness-induced transcriptome alterations and regulatory mechanisms revealed by RNA-seq in Endothelial cells
Authors: Zhang Chao , Han XiaoMei , Xu Jin , Jiang MeiLin , Zhou Yue 
Published: September 2022

Abstract
Background
Changes in vascular stiffness are associated with the development and progression of many diseases, especially in cardiovascular disease. However, the effect of vascular stiffness on the endothelial cells (ECs) is not fully understood. This study focused on the transcriptional profiles of ECs cultured on the matrices with different stiffness (1kPa and 40kPa, respectively) to broaden the knowledge of mechanotransduction.
Methods
We first prepared hydrogel materials with different stiffness to simulate the vascular stiffness in normal and pathological states, respectively. RNA sequencing was then conducted on endothelial cells cultured on matrix for 24 hours. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia for Genes and Genomes (KEGG) pathway analysis, protein–protein interaction (PPI) network analysis and transcription factors (TFs) and their target genes analysis were performed on differentially expressed genes (DEGs). qRT-PCR was finally utilized to validate the hub dysregulated genes.
Results
We obtained 1775 differentially expressed genes (DEGs) by RNA-seq, with 450 up-regulated and 1325 down-regulated DEGs in ECs cultured on soft matrix (1kPa) compared to those cultured on stiff matrix (40kPa). According to GO and KEGG analysis, DEGs were mainly enriched in cell junctions and cell-matrix interaction related signaling pathways. In order to confirm the RNA-seq results, we performed real-time qPCR analysis on the genes of interest, including cadherin 5, F11 receptor (also known as junctional adhesion molecule-A), fibronectin 1, collagen α2(IV) chain, matrix metalloproteinase-14 and integrin α5, and found that the expression level of all these genes were down-regulated on soft matrix, suggesting that soft matrix caused by pathological conditions may directly attenuate vascular barrier function.
Conclusions
In summary, the results of RNA-Seq suggested that matrix stiffness may affect extracellular matrix (ECM) integrity, cell-ECM interactions, and cell-cell junctions. This study offers the insights about the effects of physical stimulation on cells, paving a way for vascular tissue engineering, regenerative medicine, disease modeling and therapies.
Valorization of three pecan nutshell cultivars by ultrasound assisted extraction of polyphenolic compounds and its potential free radical inhibition
Authors: Garcia-Larez Frida Lourdes , Tapia-Hernández José Agustín , Quintana David Slim Zepeda , Moreno-Vásquez María Jesús , Rodríguez-Félix Francisco , Del-Toro-Sánchez Carmen Lizette 
Published: September 2022

Abstract
The present study was performed to identify the effects of ultrasound assisted extraction (UAE) conditions over total phenolic content, total flavonoid content, and antioxidant activity of pecan nutshell extracts of Western, Wichita, and Mahan cultivars. The analyzed UAE conditions were extraction time, amplitude, and sample-solvent ratio. Antioxidant activity was evaluated through ABTS, DPPH, and FRAP assays. Optimal extraction conditions were used to obtain an extract for the determination of medium inhibitory concentration (IC 50 ) and FTIR analysis. Results indicate that extraction time of 25 min favored the extraction of phenolic compounds and flavonoids; in addition, extract with an extraction time of 1 min exhibits antioxidant activity by ABTS, while extraction time of 5 min presents higher DPPH and FRAP activity. The highest concentration of phenolic compounds and flavonoids was obtained with a 50% amplitude. Antioxidant activity by ABTS was higher for an extract with 80% amplitude, while extract with 30% amplitude had higher antioxidant activity by DPPH and FRAP. Sample-solvent ratio of 1/50 had the highest content of phenolic compounds while flavonoid content was higher in the extract with a 1/30 sample-solvent ratio. Sample-solvent ratio using UAE did not have an effect over antioxidant activity by ABTS and DPPH; however, antioxidant activity by FRAP was increased by UAE were a 1/30 sample-solvent ratio exhibited the highest value. FTIR spectra suggests the presence of phenolic compounds, proteins, and polysaccharides; therefore, bioactive compounds can be recovered from pecan nutshell through ultrasound assisted extraction.
Establishment of a Visualized Mouse Orthotopic Model of Nasopharyngeal Carcinoma
Authors: Chen Wei , Chen Sixia , Jiang Li , Shu Gege , Yin Yuanxiu , Quan Zhipeng , Zhou Ziyan , Shen Mingjun , Qin Yating , Yang Chaolin , SU Xuejin , Chen Weimin , Kang Min 
Published: September 2022

Abstract
Background
Nasopharyngeal carcinoma, one of the most common head and neck tumours, is particularly prevalent in Southeast Asia and is characterized by high rates of metastasis and recurrence. Mouse orthotopic tumour models are commonly employed in studies investigating the mechanisms underlying tumour development and progression, as well as preclinical treatment. However, mature and visualized orthotopic models of nasopharyngeal carcinoma are currently unavailable, limiting the development of treatment strategies for nasopharyngeal carcinoma. The aim of the current study is to provide a simple and reliable method for building an orthotopic model of nasopharyngeal carcinoma.
Methods
human nasopharyngeal carcinoma C666-1-luc cells, stably expressing the firefly luciferase gene, were injected subcutaneously into the right axilla of BALB/C nude mice. Four weeks later, the resulting subcutaneous tumours were cut into small blocks and grafted into the nasopharynx of immunodeficient BALB/C nude mice to induce tumour formation. Tumour growth was monitored by bioluminescence imaging and small animal magnetic resonance imaging. The histological and immunological antigen expression associated with orthotopic nasopharyngeal carcinoma were analysed by tissue section analysis and immunohistochemistry (IHC).
Results
We successfully constructed a visualized orthotopic nasopharyngeal carcinoma model. Fluorescence signal detection, micro-magnetic resonance imaging and hematoxylin and eosin staining revealed the successful growth of tumours in the nasopharynx of nude mice. Moreover, IHC analysis detected positive CK, CK5/6, P40 and P63 expression in mouse orthotopic tumours, which is consistent with the reported antigen expression in the nasopharyngeal tumours of patients.
Conclusion
We established a reproducible, visualized and less lethal orthotopic model of nasopharyngeal carcinoma, providing a platform for researching nasopharyngeal carcinoma that is more conducive to preclinical research.
Ca(IO 3 ) 2 nanoparticles: fabrication and application as an eco-friendly and recyclable catalyst for green synthesis of quinoxalines, pyridopyrazines, and 2,3-dicyano pyrazines
Authors: Kalhor Mehdi , Shayestefar Mahboubeh , Khalaj Mehdi , Janghorban Fatemeh 
Published: September 2022

AbstractIn this paper, calcium iodate salt nanoparticles were first synthesized by the modified reaction of Ca(NO3)2and KIO3in aqueous media and under ultrasonic irradiation. The structure of nano-catalyst was then identified by FT-IR, FESEM, EDX, XRD, and BET techniques. Afterward, the fabricated Ca(IO3)2was applied as a nanocatalyst in the facile synthesis of heterocycles including quinoxalines, 5,6-dicyano pyrazines, and pyrido[2,3-b]pyrazines. For this purpose, the feasibility of the reaction in the presence of different catalyst amounts, solvents and temperatures was first investigated. Next, the target compounds were obtained by the condensation reaction of aryl-1,2-diamines or 2,3-diaminomaleonitrile with 1,2-diketones in the presence of catalytic amount of Ca(IO3)2in ethanol or acetic acid solvents at ambient temperature in good to excellent yields. One of the salient advantages of this work is the synthesis of calcium iodate nanoparticles by chemical precipitation method and its application as a heterogeneous nanocatalyst for the first time in the synthesis of organic compounds. The other important benefits of this process are the use of an inexpensive, safe, stable and recyclable catalyst, high yields, short reaction times, easy isolation of the product in pure form.
The relationship between substance abuse-associated stigma and deficits in executive functioning
Authors: Jahromi Leila Razeghian , Mazidi Safura Sadeghi , Bavi Azade Moradi 
Published: September 2022

Abstract
Background: Substance abuse is a common problem worldwide. Among addicts, the perceived stigma of substance abuse harms their quality of life and represents a major treatment barrier. We explored the effect of executive dysfunction on the stigma perceived due to substance abuse.
Methods: In this cross-sectional study, a validated questionnaire on executive dysfunction and stigma was distributed among eighty drug addicts admitted to Ostad Moharary Neuropsychiatric Hospital in March 2022. Demographic data were also collected. Statistical analyses were done using SPSS v. 25.
Results: In this study, 80 drug users were evaluated, of which 28 (35%) were narcotic drug users, 7 (8.8%) were stimulant drug users, and 45 (56.3%) were multidrug users. Materials were simultaneous (multiple). The mean age of participants was 36.56 37 9.37. In this study, the total test obtained from the stigma questionnaire showed a significant and positive relationship with each of the time scales of self-management, self-organization, self-regulation of emotion, self-motivation, self-inhibition, and total executive performance defects.
Conclusion: Self-perceived stigma is directly linked with executive dysfunction in drug users who are seeking to quit. Since stigma and executive function both play essential roles in addiction treatment success, interventions should be adjusted to reduce the self-perceived stigma and executive function deficits of substance addicts.
HMGCL-induced β-Hydroxybutyrate Production Attenuates Hepatocellular Carcinoma via DPP4-mediated Ferroptosis Susceptibility
Authors: Cui Xiaohan , Yun Xiao , Sun Meiling , Li Renzhi , Lyu Xiajie , Lao Yuanxiang , Yu Wenbin , Qin Xihu 
Published: September 2022

AbstractBackground:Metabolic disorder is an essential characteristic of tumor development. Ketogenesis as a heterogeneous factor in multiple cancer, but the effect of ketogenesis on hepatocellular carcinoma (HCC) is elusive.Methods:We aimed to explain a role of ketogenesis related hydroxymethylglutaryl-CoA lyase (HMGCL) on HCC suppression. Expression pattern of HMGCL in HCC specimens was evaluated by immunohistochemistry (IHC). HMGCL was depleted or overexpressed in HCC cells to investigate the functions of HMGCLin vitroandin vivo. The antitumor function of HMGCL was studied in subcutaneous xenograft andTrp53Δhep/Δhep; c-Myc-driven HCC mouse models. The mechanism of HMGCL mediated tumor suppression was studied by IHC, western blot (WB) and Cut & Tag.Results:HMGCL depletion promoted HCC proliferation and metastasis, whereas its overexpression reversed this trend. As HMGCL catalyzes β-hydroxybutyric acid (β-OHB) production, we discovered that HMGCL increased acetylation at histone H3K9, which further promoted the transcription of Dipeptidyl peptidase-4 (DPP4), a key protein maintains intracellular lipid peroxidation and iron accumulation, leading to HCC cells vulnerability to erastin- and sorafenib-induced ferroptosis.Conclusions:Our study identified a critical role of HMGCL on HCC suppression, of which HMGCL regulated H3K9 acetylation through β-OHB and modulating the expression of DPP4 in a dose-dependent manner, which led to ferroptosis in HCC cells.