bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel configurations of type I-E CRISPR-Cas system in Corynebacterium striatum clinical isolates
Authors: Ramos Juliana Nunes , Baio Paulo Victor Pereira , Veras João Flávio Carneiro , Vieira Érica Miranda Damásio , Mattos-Guaraldi Ana Luiza , Vieira Verônica Viana 
Published: February 2022

Abstract
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is an adaptative immune system that promotes the degradation of foreign nucleic acids. In this study, we analyzed 10 genomes of C. striatum clinical isolates from the public hospital in Rio de Janeiro, Brazil, deposited in the NCBI database. All isolates were submitted to antimicrobial susceptibility testing. The occurrence and diversity of the CRISPR system were investigated by bioinformatic tools. Five isolates contained only one CRISPR locus and 4 isolates contained double loci with distinct distributed sites. Our analysis revealed that the isolates exhibited Type I-E gene arrangements and 3 more multidrug-resistant isolates showed alternative gene arrangements of Type I-E not yet described. Phylogenetic analysis of the cas1 gene of this alternative arrangement named here as Type I-E’ showed a cluster in a distinct clade of the cas1 gene of Type I-E CRISPR-Cas system. The GC content of the systems is lower (52.66-54.06 mol%) than GC content of genomic DNA (59.3 mol%) and mobile genetic elements were found in some isolates near the CRISPR-Cas system providing evidence of horizontal gene transfer events. Most CRISPR spacers lack identity indicating that there is a reservoir of unexplored corinebacteriophages and plasmids. Intact phage regions were found in 3 of our isolates ranging from 9.1 kb to 43.8 kb, with regions showing similarity to Rhodococcus and Corynebacterium phages. Our results may contribute to researches about the diversity of the CRISPR-Cas system in C. striatum, where there is still no data published until the moment.
Hematoma Cavity Separation and Neomembrane Thickness are Potential Triggers of Recurrence of Chronic Subdural Hematoma
Authors: Liu Hongbin , Yan Rudan , Xie Fei , Richard Seidu A. 
Published: February 2022

Abstract
Background: Chronic subdural hematoma (CSDH) is the anomalous accumulation of liquified blood in the subdural space usually after a head injury in the elderly. Almost all the research on surgical techniques and endoscopic assisted evacuation of CSDH focused on the just the evacuation and not abnormal anatomical structures that causes recurrences. Objectives: We investigated abnormal anatomical structures that triggers recurrence of CSDH during craniotomy as well as burr-hole craniostomy with endoscopic assistance. Materials and Method: We retrospectively analyzed all patients with CSDH who underwent craniostomy and burr-hole craniotomy with endoscopic assisted evacuation of hematoma between April 2017 and November 2020 at our institution. Clinical data obtained was categorized into patient-related, radiology as well as surgery and endoscopic evaluations. Results: A total of 143 patients (109 men and 34 women) aged 43-94 years (mean age, 68.35 years) with CSDH were included in this study. We observed a recurrence rate of 4.9% (7/143). Recurrences occurred between 2-6 months after the operation in patients with recurrences.Our data revealed that, age, hypertension, history of injury, diabetes, antiplatelet or anticoagulant use were not associated with hematoma recurrence. Nevertheless, all the patients with recurrence of hematoma were males. Interestingly, our univariate and multivariate analyses found neomembrane thickness and hematoma cavity separation as independent risk factors (OR,45.822; 95% CI,2.666-787.711; p=0.008) for the recurrence of CSDH (p<0.05). Also, we observed thickened membranes connecting/separating the dura and the thickened arachnoid/pia matters in all the 7 patients with hematoma recurrence. Conclusion: The treatment of patients with CSDH ought to include the identification and resection of abnormal thickened membranes connecting/separating the dura and the thickened arachnoid/pia matters to avoid recurrence. Comparatively, endoscopy showed hematoma cavity separation or neomembrane thickness just as seen during craniotomy.
Exploration of student’s social presence in a web-based discussion for conceptual learning of organic chemistry
Authors: Iyamuremye Aloys , Mukiza Janvier , Nsabayezu Ezechiel , Kwitonda Jean Dieu , Habimana Claude 
Published: February 2022

Abstract
The current paper reports social presence in web-based discussion for their conceptual learning of organic chemistry class. The study examined students-students, students-instructor, and students-leaning materials interactions via the designed web-based discussion. We developed an online forum nicknamed” Universal Chemistry Network to enhance communication among students and teachers in organic chemistry class. A sample of 138 senior five students was purposively selected from two secondary schools located in Kicukiro district, Eastern province in Rwanda. A questionnaire composed of multiple-choice and open-ended questions was used to collect data. Descriptive statistics and interpretive analysis were used to analyze data. The results revealed that the use of web-based discussion is the potential and effective teaching method for enhancing students' -student’s and student’s- teacher’s interaction in organic chemistry class also students appreciated this teaching and learning method as it helped them to search for additional information, exchange their ideas, knowledge, and experiences. A web-based discussion was also found to provide the students with the potential confidence and motivation to express freely their ideas. The challenges faced by students during web-based discussion while learning organic chemistry were also examined
Covid-19 and Rural Educational Systems of Goa, India: A Descriptive Short Report of a Government School in Sattari, Goa
Authors: Fale Dattaram , D’Souza Nezlyn Cressida 
Published: February 2022

Abstract
The devastating Sars-cov-2 outbreak of 2020 has affected all functioning systems of the world, as well as highlighted the loopholes in existing systems. As with other services, the educational systems across the globe have been drastically affected. Being a country of people of diverse socioeconomic backgrounds, India, a country that has the world’s second-largest school system, has seen a disproportionate effect of the pandemic and the restrictions imposed on the teaching-learning systems of rural and urban environments. As the country suffered through multiple devastating waves of the Covid-19 pandemic, it imposed the longest-running closures of schools. Like other states of India, the education systems of Goa, a lush green state on the West coast of India, has also been affected by the pandemic and related modification of regulations. The rural schools of Goa have been disproportionately affected by the pandemic as compared with schools in urban environments. This paper discusses the case of a government school in Sattari-North Goa, enlightens the challenges faced and initiatives that were taken to tackle the situation.
Extra-Gastrointestinal Stromal Tumors (EGISTs): A Case Report for A Mischief Entity
Authors: Charif Mohamad Hadi El , Amro Sara , Boulos Fouad , Khalife Mohamad , Shamseddine Ali , Assi Hazem , Sbaity Eman 
Published: February 2022

Abstract
Background: Extra-gastrointestinal stromal tumor (EGIST) is a rare subtype of soft tissue sarcomas with significantly variable presentation, management and prognosis. This makes it crucial to report the different institutional experiences of encountering EGIST.Case Presentation: We report three cases of EGIST diagnosis at our institution for 2 males and 1 female in the 5th, 6th and 7th decades of life, respectively. For the first case, the tumor was initially suspected to be ovarian cancer but biopsy revealed a diagnosis of EGIST and the patient was started on neoadjuvant therapy. In the second case, the tumor was retrogastric and prelim diagnosis was gastric cancer but again biopsy revealed an EGIST histopathology and the patient underwent surgery and adjuvant treatment. For the third case, a previous history of testicular cancer prompted an initial suspicion of recurrence with metastasis but biopsy and immunohistochemistry staining revealed EGIST with related markers. The patient underwent treatment at a different institution in his home country.Conclusion: This report sheds light on the importance of keeping EGIST amongst any differential list for abdominal and pelvic tumors. It also shows that EGIST-focused studies are needed to assess the effectiveness of the different treatment modalities available when utilized specifically for EGIST. This would allow for better oncological outcomes and improved quality of life.
Clinicopathological characteristics of neuroendocrine differentiated invasive breast cancer with simultaneous gastric, colon and lung metastases: a case report
Authors: Wang Yang-kun , Chen Xiao-dong , Liao Xing-hai , Yang Bin-Feng 
Published: February 2022

Abstract
Background: To investigate the clinicopathological characteristics of infiltrating ductal carcinoma of the breast with neuroendocrine differentiation and metastasis to the stomach, colon and lung at the same time. Methods: To The histopathological characteristics and immunophenotype of a case of breast invasive ductal carcinoma with neuroendocrine characteristics and concurrent gastric, colon and lung metastases were observed. Results: The histological structure and cell morphology of the primary lesions and metastatic lesions of breast invasive ductal carcinoma with neuroendocrine differentiation were basically the same, and they were all non-lumen invasive ductal carcinoma of the breast. The antibody markers of this case of breast invasive ductal carcinoma were as follows: the primary and metastatic foci were all positive for E-cad, p120 and ER, while PR, HER2, GATA3, Mammaglobin and GCDFP-15 were all negative. The antibody markers of non-breast tissues were as follows: TTF1, CgA and Sym were positively expressed in primary and metastatic foci. This tumour was extremely rare and had characteristic histomorphological changes. Conclusion: The diagnosis of invasive breast carcinoma with neuroendocrine differentiation should be based on histology and cell morphology, CgA and SyN-positive expression, mitosis and cell proliferation index ki-67 positive cell comprehensive analysis, in order to improve understanding and early diagnosis and avoid missed diagnosis and misdiagnosed cases.
Perspective on the Optimal Diagnostic Approach for Dysphagia Considering Wearing-off Phenomenon in a Patient with Parkinson’s Disease: A Case Report
Authors: Kim Kyoung Tae 
Published: February 2022

Abstract
BackgroundDysphagia is a major disability in patients with Parkinson's disease. Unlike its motor symptoms, dysphagia is relatively unresponsive to dopaminergic drugs. However occasionally, swallowing difficulty is significantly affected by the wearing off phenomenon. In such cases, it is difficult to accurately assess the severity of dysphagia if evaluation of swallowing is performed without considering the on-period based on dopaminergic medication.Case presentationHere, we report a case of dysphagia in a patient with Parkinson’s disease severely affected by the wearing-off phenomenon. The patient presented severe oral phase delay with choking symptoms during fluid intake. Massive aspiration was observed on routine scheduled videofluroscopic swallowing study, and an alternative feeding method and maximum diet restriction were prescribed. However, when swallowing evaluation was performed according to the on-period, almost no abnormal function was observed, and after the on/off period-tailored food intake prescription, sufficient nutrition was possible without aspiration.ConclusionIn swallowing evaluation for dysphagia in patient with Parkinson's disease, it is essential to consider the wearing-off phenomenon. Patient-specific swallowing evaluation and diet prescription is needed to establish optimal therapeutic strategies.
The First Case of Clear Cell Sarcoma of Soft Tissue with Purely Plasmacytoid Morphology: A Case Report
Authors: Han Xu , Li Qingchang , Wang En-Hua , Liu Nan 
Published: February 2022

Abstract
Clear cell sarcoma of soft tissue (CCSST) is a rare malignant tumor occurring in the extremities of young adults. The tumor cells arranged in nests and sheets, with clear to eosinophilic cytoplasm separated by fibrous septa. CCSST has been documented to have atypical histopathological features, such as epidermotropism or myxoid differentiation, which may set pitfalls in differential diagnosis. Herein, we report a case of CCSST with purely plasmacytoid morphology which has never been described. The patient, a 15-year-old male, presented with a 5-cm mass in his left inguinal area. He received cryotherapy for a “black mole” on his back two years ago without pathology record. Positron emission tomography-computed tomography examination showed nodules in the left groin and the lung, the latter was considered as metastasis. Needle biopsy of inguinal mass showed tumor cells were uniformly sized, with eosinophilic cytoplasm and eccentric, basophilic nuclei, resembling plasmocytes. Immunostaining showed strong expression of S-100, HMB45, Melan-A, and MUM-1 in all tumor cells, while CD79a and CD38 were negative. Genetic analysis revealed the tumor harboring no BRAF mutations but EWSR1 rearrangement. Accordingly, the diagnosis of CCSST with plasmacytoid morphology was made. This is the first case of CCSST with purely plasmacytoid morphology which should be aware during evaluating and differentiating CCSST from tumors with similar morphology, such as plasmacytoid melanoma and extramedullary plasmacytoma, etc.
The Effect of Web-Based Education Programs on Self-Efficacy and Self-Care Behavior in Quality of Life Among Diabetic Type 2 Patients in Public Hospital
Authors: MALEK TENGKU MOHD MIZWAR BIN T , Ahmad Aini Binti 
Published: February 2022

Abstract
Background: Changing to an intervention method in health education is a very challenging process and requires various transformations. Complications from the increasingly complicated diagnosis of type 2 diabetes cause diabetic patients to experience a deterioration in aspects of diabetic self-efficacy management (DSEM), diabetic self-care behavior management (DSCM) in their quality of life (QoL). Unsuitable environmental factors, increased amputation rates, and lack of information resources of choice for patients, researchers have innovated in type 2 diabetic health education through web-based Diabetic-N-Care education as a moderator to DSEM, DSCM, and QoL and as a method, an alternative to face to face education in Hospital Sultanah Nur Zahirah Kuala Terengganu.Objective: This study aims to identify the effects of web-based educational programs on diabetic self-efficacy management (DSEM), diabetic self-care behavior management (DSCM) in quality of life (QoL) among type 2 diabetes patients in public hospitals.Method: This study used a quantitative quasi-experimental design of pre-test and post-test. Diabetic-N-Care program was conducted at Orthopedic Clinic Hospital Sultanah Nur Zahirah Kuala Terengganu for 34 days, where purposive sampling involved type 2 diabetic patients who were divided into intervention groups (IG) (n = 60) and control group (CG) (n = 60). Respondents are the same individual for each phase of measurement.Results: Data analysis method the general linear model repeated measures ANOVA, Split-plot ANOVA (SPANOVA) and paired t-test was conducted on 120 patients to see the effect of using Diabetic-N-Care on IG. The results of Split - plot ANOVA analysis showed a significant overall effect of DSEM, DSCM, and QoL (p = 0.000) on IG. Meanwhile, paired t-test analysis there was a significant mean difference in DSEM, DSCM, and QoL at pre-test and post-test (p = 0.000) to IG compared to CG.Conclusion: Web-based health education can have an impact on DSEM, DSCM in the QoL of type 2 diabetic patients were greater than before confidence, change the old behavior to new behavior to improve quality of life in the long term planning. Therefore, this study concludes that web-based methods such as Diabetic-N-Care need to be widely adapted in current health education methods.
Joint Modeling of Longitudinal Changes of Respiratory Rate, Pulse Rate and Oxygen Saturation with Time to Convalescence among Pneumonia Patients: A comparison of separate and joint models
Authors: Wube Yenefenta , Azmeraw Sindu 
Published: February 2022

Abstract
Background: Globally, pneumonia is the first infectious disease which is the leading cause of children under age five morbidity and mortality with 98% of deaths in developing countries.Objective: The study aimed to identify the determinant factors that jointly affect the longitudinal measures of pneumonia (respiratory rate, pulse rate and oxygen saturation) and time to convalescence or recovery of under five admitted pneumonia patients at Felege Hiwot Referral Hospital, Bahir Dar, Ethiopia.Methods:A prospective cohort study design was used on 101 sampled under five admitted pneumonia patients from December 2019 to February 2020. The study was conducted using joint model of longitudinal outcomes and survival outcomes.Results: The significant values of shared parameters in the survival sub model shows that the use of joint modeling of multivariate longitudinal outcomes with the time to event outcome is the best model compared to separate models. The estimated values of the association parameters for \({\gamma }\_1,\) \({\gamma }\_2 \text{a}\text{n}\text{d} {\gamma }\)_3 were -0.297, -0.121 and 0.5452 respectively and indicates that; respiratory rate and pulse rate were negatively related with recovery time, whereas oxygen saturation was positively associated with recovery time. As age of patients increased by one month, the average respiratory rate and pulse rate were significantly decreased by 0.3759 bpm and 1.1012 bpm respectively keeping other variables constant, but age has no information about oxygen saturation.Conclusion: Residence, birth order, severity and visit were found as determinants of the longitudinal measures of pneumonia and time to recovery of under-five admitted pneumonia patients jointly. To improve child survival, the community should be responsible for post ponding child birth and marriage.
Expression Of Serum Cytokines Profile In Neonatal Sepsis
Authors: Kuang Mengjiao , Chen Suipeng , Qu Ying , Huang Shirui , Gong Binbin , Lin Suzhen , Wang Huiyan , Wang Guiye , Tao Hongqun , Yu Jian , Yang Zuqin , Jiang Minghua , Xie Qipeng 
Published: February 2022

Abstract
Objectives: Sepsis remains a major cause of neonatal death. In order to better characterize the inflammatory response during neonatal sepsis, we compared the differences in cytokines and chemokines between children with neonatal sepsis and full-term neonates without infection.Methods: We enrolled 40 full-term neonates with sepsis and 26 full-term neonates without infection as controls between October 2016 and June 2018. Forty cytokines /chemokines in serum were analyzed using the Luminex Bead Immunoassay System.Results: Our results showed that serum IL-6, IL-8, TNF-α, IL-1β, MIF, CXCL13, CXCL1, CXCL2, CXCL5, CXCL6, CXCL16, CCL27, CCL2, CCL8, CCL3, CCL20, CCL23, CCL27, and CX3CL1 levels were significantly increased in neonates with sepsis compared to those in the control group (all adjusted p<0.1). The levels of serum CCL20, and IL-17 were higher in late-onset sepsis (LOS) than those in early-onset sepsis (EOS) (all adjusted p<0.1). Conversely, serum CXCL16 was lower in LOS than that in EOS (adjusted p<0.05).Conclusions: Our findings revealed that excessive pro-inflammatory cytokines might be involved in neonatal sepsis. In addition, chemokines significantly increased the recruitment of immune cells after infection to participate in the anti-infection defense of neonates, but this could lead to damage.
Classification and Yield Prediction in Smart Agriculture System Using IoT
Authors: Gupta Akanksha , Nahar Priyank 
Published: February 2022

Abstract
The Modern agriculture industry is data-centred, precise and smarter than ever. Advanced development of Internet-of-Things (IoT) based systems redesigned “smart agriculture”. This emergence in innovative farming systems is gradually enhancing the crop yield, reduces irrigation wastages and making it more profitable. Machine learning (ML) methods achieve the requirement of scaling the learning performance of the model. This paper introduces a hybrid ML model with IoT for yield prediction. This work involves three phases : pre-processing, feature selection(FS) and classification. Initially, the dataset is pre-processed and FS is done on the basis of Correlation based FS (CBFS) and the Variance Inflation Factor algorithm (VIF). Finally, a two-tier ML model is proposed for IoT based smart agriculture system. In the first tier, the Adaptive k-Nearest Centroid Neighbour Classifier (aKNCN) model is proposed to estimate the soil quality and classify the soil samples into different classes based on the input soil properties. In the second tier, the crop yield is predicted using the Extreme Learning Machine algorithm (ELM). In the optimized strategy, the weights are updated using modified Butterfly Optimization algorithm (mBOA) to improve the performance accuracy of ELM with minimum error values. PYTHON is the implementation tool for evaluating the proposed system. Soil dataset is utilized for performance evaluation of the proposed prediction model. Various metrics are considered for the performance evaluation such as accuracy, RMSE, R2, MSE, MedAE, MAE, MSLE, MAPE and Explained Variance Score (EVS).
Improving the Performance of HF Radio Networks in Presence of Interference: Frequency Hopping Automatic Link Establishment
Authors: Haghbin Afrooz , Khodaverdizadeh Masoud , Razzazi Farbod 
Published: February 2022

Abstract
Automatic Link Establishment (ALE) is crucial in channel assignment and data exchange in HF radio networks. The time of link establishment and its quality are two main concerns in this criterion establishment. In jamming environments, the quality of the channels is seriously influenced by the jammer signals. Frequency Hopping (FH) approaches supports communication links to overcome the jamming impairments, and the traditional ALE algorithm can be made more stable by using this approach. This paper has developed ALE algorithms to automatically establish links to handle different jamming scenarios and establish robust links to exchange data with the proposed FH-ALE system. Besides, in the proposed algorithm, we have considered the time of link establishment to be almost identical to the current ALE scenario. Hence, in the normal channel condition, the throughput value is approximately identical in proposed and traditional cases. The simulation results represent the superiority of the proposed FH-ALE approach in jamming environments.