bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a Standardized Protocol for Plasma Rich in Growth Factors Obtention in Cats: A Prospective Study
Authors: Miguel-Pastor Laura , Satué Katy , Chicharro Deborah , Torres-Torrillas Marta , del Romero Ayla , Peláez Pau , Carrillo José M. , Cuervo Belén , Sopena Joaquín J. , Cerón José J. , Rubio Mónica 
Published: April 2022

IntroductionPlatelet-rich plasma (PRP) is an autologous plasma with platelet (PLT) concentration above that of whole blood (WB). PLTs contain growth factors (GFs) that promote tissular repair.ObjectivesTo determine and compare the concentrations of PLT, red blood cells (RBC) and white blood cells (WBC) between WB samples, PRP and platelet poor plasma (PPP) samples; and to analyze the concentrations of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor β1 (TGF-β1) in the PRP and PPP of healthy adult cats using a standardized protocol with PRGF®-Endoret® characteristics.Material and MethodsWB was collected from 30 cats. PRP was obtained following three centrifugation protocols using PRGF®-Endoret® technology: 255, 260, and 265 g for 10 min each. The cellular components, RBC, WBC, PLT, and the concentrations of PDGF-BB and TGF-β1 in the PRP and PPP fractions were determined for each protocol.ResultsPLTs in the PRP fraction were statistically higher than WB, with no statistical differences between PPP and WB. In PRP fraction, PLT concentration was increased 1.4 times on average at 255 g; 1.3 times at 260 g and, 1.5 times at 265 g without statistical differences among them. The mean platelet volume (MPV) was significantly higher in WB compared to PRP and PPP fractions without significant differences between protocols. Compared to WB, the number of RBCs and WBCs was reduced by 99% and by more than 95% in PRP and PPP respectively, without significant differences between protocols. PDGF-BB concentrations were statistically higher in PRP than in PPP fractions, however, TGF-ß1 concentrations did not vary between fractions at 260 g. Comparing the three protocols within PRP and PPP fractions, no differences in PDGF-BB and TGF-ß1 concentrations were observed.Clinical RelevanceThe study shows scientific evidence regarding the obtention of PRP in cats using the PRGF®-Endoret® technology for the quantification of PDGF-BB and TGF-ß1. At 265 g for 10 min, PLT concentration was increased 1.5 times with unnoticeable erythrocytes and leukocytes in the samples. These results clearly show that the PRGF®-Endoret® methodology is suitable to obtain PRP in cats. Further studies are needed to determine the clinical efficacy of the obtained PGRF in the treatment of different pathologies in cats.
Mortality Burden due to Exposure to Outdoor Fine Particulate Matter in Hanoi, Vietnam: Health Impact Assessment
Authors: Nhung Nguyen T. T. , Jegasothy Edward , Ngan Nguyen T. K. , Truong Ngo X. , Thanh Nguyen T. N. , Marks Guy B. , Morgan Geoffrey G. 
Published: April 2022

Objective: This study reports the mortality burden due to PM2.5 exposure among adults (age &gt;25) living in Hanoi in 2017.Methods: We applied a health impact assessment methodology with the global exposure mortality model and a PM2.5 map with 3 × 3 km resolution derived from multiple data sources.Results: The annual average PM2.5 concentration for each grid ranged from 22.1 to 37.2 µg/m³. The district average concentration values ranged from 26.9 to 37.2 µg/m³, which means that none of the 30 districts had annual average values below the Vietnam Ambient National Standard of 25 µg/m3. Using the Vietnam Ambient National Standard as the reference standard, we estimated that 2,696 deaths (95% CI: 2,225 to 3,158) per year were attributable to exposure to elevated PM2.5 concentrations in Hanoi. Using the Interim Target 4 value of 10 µg/m3 as the reference standard, the number of excess deaths attributable to elevated PM2.5 exposure was 4,760 (95% CI: 3,958–5,534).Conclusion: A significant proportion of deaths in Hanoi could be avoided by reducing air pollution concentrations to a level consistent with the Vietnam Ambient National Standard.
Apoptotic p53 Gene Expression in the Regulation of Persistent Organic Pollutant (POP)-Induced Oxidative Stress in the Intertidal Crab Macrophthalmusjaponicus
Authors: Park Kiyun , Kwak Ihn-Sil 
Published: April 2022

Persistent organic pollutants (POPs), some of the most dangerous chemicals released into the aquatic environment, are distributed worldwide due to their environmental persistence and bioaccumulation. In the study, we investigated p53-related apoptotic responses to POPs such as hexabromocyclododecanes (HBCDs) or 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) in the mud crab Macrophthalmus japonicus. To do so, we characterized M. japonicus p53 and evaluated basal levels of p53 expression in different tissues. M. japonicus p53 has conserved amino acid residues involving sites for protein dimerization and DNA and zinc binding. In phylogenetic analysis, the homology of the deduced p53 amino acid sequence was not high (67–70%) among crabs, although M. japonicus p53 formed a cluster with one clade with p53 homologs from other crabs. Tissue distribution patterns revealed that the highest expression of p53 mRNA transcripts was in the hepatopancreas of M. japonicus crabs. Exposure to POPs induced antioxidant defenses to modulate oxidative stress through the upregulation of catalase expression. Furthermore, p53 expression was generally upregulated in the hepatopancreas and gills of M. japonicus after exposure to most concentrations of HBCD or BDE-47 for all exposure periods. In hepatopancreas tissue, significant increases in p53 transcript levels were observed as long-lasting apoptotic responses involving cellular defenses until day 7 of relative long-term exposure. The findings in this study suggest that exposure to POPs such as HBCD or BDE-47 may trigger the induction of cellular defense processes against oxidative stress, including DNA repair, cell cycle arrest, and apoptosis through the transcriptional upregulation of p53 expression in M. japonicus.
Atlantic Salmon Mucins Inhibit LuxS-Dependent A. Salmonicida AI-2 Quorum Sensing in an N-Acetylneuraminic Acid-Dependent Manner
Authors: Padra János Tamás , Loibman Stefany Ojaimi , Thorell Kaisa , Sundh Henrik , Sundell Kristina , Lindén Sara K. 
Published: April 2022

One of the most important bacterial diseases in salmonid aquaculture is furunculosis, caused by Aeromonas salmonicida. Bacterial communication through secreted autoinducer signals, quorum sensing, takes part in the regulation of gene expression in bacteria, influencing growth and virulence. The skin and mucosal surfaces, covered by a mucus layer, are the first point of contact between fish and bacteria. Mucins are highly glycosylated and are the main components of mucus. Here, we validate the Vibrio harveyi BB170 bioreporter assay for quantifying A. salmonicida quorum sensing and study the effects of Atlantic salmon mucins as well as mono- and disaccharides on the AI-2 levels of A. salmonicida. Atlantic salmon mucins from skin, pyloric ceca, proximal and distal intestine reduced A. salmonicida AI-2 levels. Among the saccharides abundant on mucins, fucose, N-acetylneuraminic acid and GlcNAcβ1-3Gal inhibited AI-2 A. salmonicida secretion. Removal of N-acetylneuraminic acid, which is the most abundant terminal residue on mucin glycans on Atlantic salmon mucins, attenuated the inhibitory effects on AI-2 levels of A. salmonicida. Deletion of A. salmonicida luxS abolished AI-2 production. In conclusion, Atlantic salmon mucins regulate A. salmonicida quorum sensing in a luxS and N-acetylneuraminic acid-dependent manner.
Computer Aided Screening Method for Diabetes using Thermogram
Authors: Gayatri Joshi  , Punal M Arabi  
Published: April 2022

Diabetes is a chronic disease occurring due to insufficient production of insulin by the pancreas or the body’s ineffective usage of the insulin produced. The former leads to Type 1 diabetes whereas the latter leads to Type 2. Diabetes is estimated to have affected 422 million people globally and 69.2 million people in India annually. Diabetes if left untreated can cause serious health complications such as peripheral nerve dysfunction, diabetic retinopathy, diabetic foot and also doubles the risk of early death, thus making early diagnosis very essential for better disease management, thus improving the quality of life of the patient.
This papers aims at proposing a non invasive method to diagnose diabetes which is based on the thermoregulation of the peroneal blood vessel. The thermograms of the peroneal vessel after a cold stress are obtained and the mean pixel intensities are found and analyzed. The results found show the feasibility diabetic screening using this method.
Use of Hyperbaric Oxygenation in the Treatment of Eye Diseases
Authors: Дроздов В.А. , Сакович В.Н. , Сакович Е.Ф. , Янушкевич К.С. 
Published: April 2022

В патогенезе различных глазных заболеваний ишемические и гемодинамические изменения приводят к гипоксии, которая трансформирует клетки в состояние парабиоза. Гипоксия является одной из причин, нарушающих процессы биологического окисления, что может привести к развитию энергетической недостаточности клеток и тканей. За последнее время повысилась заинтересованность к немедикаментозным методам лечения гипербарической оксигенации, которая существенно снижает фармакологическую нагрузку на пациента, при этом активно влияет на патологический процесс и способствует улучшению функционального состояния глаз. По данным Американского общества подводной и гипербарической медицины, использование гипербарической оксигенации рекомендуется в лечении следующих глазных заболеваний: окклюзии артерий сетчатки, декомпрессионной болезни с глазными проявлениями, газовой эмболии сосудов глаза, орбитальной клостридиальной гангрены, орбитального мукоромикоза, а также при трансплантации кожи век или периорбитальной области. Данные заболевания имеют высокий доказательный уровень безопасности и эффективности использования гипербарической оксигенации при проведении комбинированного лечения. Дальнейшие исследования комплексного применения гипербарической оксигенации в лечении других глазных болезней позволят улучшить качество и снизить длительность лечения, а также повысить качество жизни пациентов. В данной статье собран литературный обзор исследований применения гипербарической оксигенации в лечении глазных болезней.
In the pathogenesis of various eye diseases ischemic and hemodynamic changes leading to hypoxia, which transforms cells into a state of parabiosis. Hypoxia is one of the reasons that change the processes of biological oxidation, which can lead to the development energy deficiency of cells and tissues. Recently interest in non-drug methods of treatment like hyperbaric oxygenation has increased, because it significantly reduces the pharmacological load on the patient while actively affecting the pathological process and contributing to the improvement of the functional state ofthe eye. According to the American Society of Underwater and Hyperbaric Medicine, the use of hyperbaric oxygenation is recommended in the treatment of the following eye diseases: occlusion of the retinal arteries, decompression sickness with eye manifestations, arterial gas embolism of the eye vessels, ocular and periocular gas gangrene, orbital mucoromycosis, radiation optic neuropathy, periorbital reconstructive surgery. These diseases have a high evidence level of safety and effectiveness using hyperbaric oxygenation during combined treatment. Further studies of the integrated use of hyperbaric oxygenation in the treatment of other eye diseases should improve the quality of treatment, reduce the duration of treatment and improve the quality of life patients. This article contains a literature review of studies using hyperbaric oxygenation in the treatment of eye diseases.
The Effectiveness of the Original Method of Inferior Oblique Recession on Superior Oblique Palsy in Children with Horizontal Vertical Strabismus
Authors: Дундаров З.А. , Коломыцкая Е.О. , Дравица Л.В. 
Published: April 2022

Проведен анализ эффективности разработанного способа коррекции вертикального косоглазия, влияния его на тортиколлис и гиперфункцию нижней косой мышцы у пациентов с горизонтально-вертикальным косоглазием, оперированных в ИМФУП Медицинский центр Новое зрение в 20082017 годах. Полученные данные сравнивались с результатами хирургического лечения детей, прооперированных традиционным способом. Разработанный нами метод устраняет вертикальную девиацию вдаль в 92,08 случаев (р0,004) и вблизи в 91,09 случаев (р0,009). Метод позволяет уменьшить степень гиперфункции нижней косой мышцы в приведении в 72,28 случаев (р0,012) и устранить третью степень глазного тортиколлиса.
To study the surgical effects of the developed method for the correction on the reduction of vertical deviation, torticollis and of inferior oblique muscle overaction in children who had horizontal- vertical strabismus. Patients were operated in the Medical center New vision between 2008 and 2017 years. The method we developed eliminates vertical deviation into the distance in 92.08 of cases (p0.004) and close in 91.09 of cases (p0.009). The method allows to reduce the degree of inferior oblique muscle overaction (IOOA) in 72.28 (p0.012) and eliminate the third degree of ocular torticollis.
Local Immunity of the Mucous Membrane of Oral Cavity and Colonization’s Resistance
Authors: Савичук Н.О. , Трубка И.А. , Савичук А.В. , Корниенко Л.В. , Ермакова Л.Г. 
Published: April 2022

Слизистые оболочки, в том числе и слизистые оболочки полости рта, являются первым защитным барьером между окружающей средой и внутренней средой человека. Состояние и функциональная способность слизистой оболочки полости рта определяют риск возникновения заболеваний как полости рта, так и организма в целом. Основываясь на результатах собственных исследований о состоянии микроэкологии полости рта у здоровых детей и у детей с различными формами заболеваний слизистой оболочки полости рта, нами разработана классификация степеней тяжести нарушений колонизационной резистентности полости рта. Состояние микроэкологии полости рта является очень важным фактором, определяющим уровень стоматологического здоровья и риск возникновения стоматологических заболеваний. Состояние колонизационной резистентности полости рта необходимо оценивать и учитывать во время обследования пациента и планирования комплекса лечебно-профилактических мероприятий.
The mucous membranes, including the mucous membranes of the oral cavity, are the first protective barrier between the environment and the internal environment of a person. The condition and functional ability of the oral mucosa determine the risk of both diseases of the oral cavity and the body as a whole. Based on the results of our own studies on the state of the oral microecology in healthy children and in children with various forms of diseases of the oral mucosa, we developed a classification of the severity of violations of the colonization resistance of the oral cavity. The state of the oral microecology is a very important factor determining the level of dental health and the risk of dental diseases. The state of colonization resistance of the oral cavity must be assessed and taken into account during the examination of the patient and the planning of a set of therapeutic and preventive measures.
Allergic Reaction to β-lactam Antibiotics – Carbapenems (Literature Review and Clinical Case Report)
Authors: Лапцевич А.В. , Кондаурова С.Л. , Липницкий А.В. 
Published: April 2022

Карбапенемы имеют наиболее широкий спектр активности среди всех -лактамных антибиотиков и играют важную роль в лечении различных инфекционных заболеваний. С учетом их значения в клинической практике информация о вероятности развития побочных реакций имеет важное значение для клиницистов. Целью данной статьи является обзор текущей литературы и описание собственного клинического случая аллергических реакций на карбапенемы, частоты возникновения реакций, факторов риска и возможности перекрестной чувствительности внутри группы карбапенемов. По данным постмаркетинговых исследований, частота возникновения сыпи, зуда, крапивницы на карбапенемы составляет 0,33,7. Из-за низкой частоты встречаемости к настоящему времени не определены факторы риска, по которым можно предсказать вероятность развития аллергии на карбапенемы. Данные о перекрестной чувствительности между карбапенемами и другими представителями -лактамных антибиотиков и карбапенемами ограничены описанием отдельных случаев, однако риск таких осложнений низкий. На данный момент нет полных исследований, описывающих частоту встречаемости перекрестных реакций внутри самой группы карбапенемов.
Carbapenems possess the broadest spectrum of activity of the beta-lactams and considered crucial for management of various infectious diseases. Taking into account the important role of carbapenems in clinical practice, understanding carbapenem side effects is essential for clinicians. The purpose of this review article is to provide a review of carbapenem allergies, describing the overall incidence, risk factors, and the possibility of cross sensitivity in the group of carbapenems. The reported incidence of rash, pruritus, and urticaria was 0.33.7 in post-marketing studies of carbapenems. Taking into account the low incidence reported in post-marketing studies, the risk factors that predispose patients to carbapenem allergy have not been described in the literature. The data on cross sensitivity between -lactam antibiotics and carbapenems are limited. However, the risk of such complications is low. There are no clinical studies that have described cross sensitivity between the carbapenem agents.
Penerapan Audit Sistem Informasi E-Raport pada SMAN 8 Padang menggunakan Metode Framework Cobit 4.1
Authors: Wijaya Dani Candra , Erdisna E , Rizki Syafrika Deni 
Published: April 2022

Abstract SMAN 8 Padang has implemented an online E-Raport Information System for 4 years and there have been changes and improvements that allow errors to occur in the system. Therefore, it is necessary to conduct an audit for the development of the E-Raport Information System in the future. And one of the measuring tools in Information System Audit is COBIT (Control Objectives For Information & Related Technology). This research was conducted by measuring the object of research including the measurement of maturity level using primary IT Processes from 4 domains contained in Cobit Audit (PO9, A12, DS5, and ME2). Based on the research, the maturity level of the E-Raport Information System at SMAN 8 Padang has reached a well-managed level.
Keywords : Information System Audit, E-Raport, COBIT 4.1 Framework, SMAN 8 Padang.
AbstrakSMAN 8 Padang telah menerapkan Sistem Informasi E-Raport secara online selama 4 Tahun dan telah terjadi perubahan dan perbaikan yang memungkinkan terjadinya kesalahan pada sistem. Oleh karena itu perlu dilakukan audit untuk pengembangan Sistem Informasi E-Raport dimasa yang akan datang. Dan salah satu alat ukur dalam audit SI adalah COBIT (Control Objectives For Information & Related Technology). Penelitian ini dilakukan dengan cara mengukur terhadap objek penelitian meliputi pengukuran maturity level menggunakan primary IT Processes dari 4 domain yang terdapat pada Cobit Audit ( PO9, A12, DS5, dan ME2 ). Berdasarkan penelitian, tingkat kemantangan Sistem Informasi E-Raport pada SMAN 8 Padang telah mencapai tingkat terkelola dengan baik.
Kata Kunci : Audit Sistem Informasi, E-Raport, Framework COBIT 4.1, SMAN 8 Padang.
Analisis Perkembangan Psikologis Anak Yang Tumbuh Dalam Keluarga Step Parent
Authors: Tabroni Imam , Mukholid Agus , Silawati Silawati 
Published: April 2022

<p><em>Keluarga adalah bagian terpenting dalam proses pertumbuhan dan perkembangan anak, dalam keluarga anak mendapatkan pendidikan serta dukungan dalam kehidupannya sehari-hari. Tetapi dalam beberapa kasus, mengharuskan orang tua bercerai dan menikah kembali, sehingga anak memiliki orangtua tiri. Penelitian ini bertujuan </em><em>untuk mendeskripsikan perkembangan psikologis anak yang tumbuh dalam keluarga step parent. Pendekatan penelitian adalah menggunakan pendekatan kualitatif dengan metode deskriptif.</em><em> Jenis penelitian yang digunakan adalah studi kasus. informan dari penelitian adalah tiga orang anak dengan usia di bawah 17 tahun. Pengumpulan data dilakukan dengan wawancara, observasi dan dokumentasi.</em><em> Teknik analisis data dilakukan dengan reduksi data, penyajian data, dan verifikasi. Hasil penelitian menunjukkan bahwa proses perkembangan psikologis anak yang tumbuh dalam keluarga step parent dapat berkembang baik seperti anak yang tumbuh dalam keluarga pada umumnya, tergantung pada perlakuan orangtua tiri kepada anak dan penerimaan diri anak terhadap orangtua tirinya. Jika orang tua tiri mampu membangun bonding dengan anak, maka anak akan merasa keluarganya utuh seperti keluarga lain. Sebaliknya, jika orangtua tiri tidak mampu membangun bonding dengan anak, anak akan cenderung menutup diri dan menolak keadaan, sehingga dapat menghambat komunikasi anak dengan orang tua.</em></p>
ASSESSING THE IMPACT OF AIR PASSENGER TRAFFIC IN AIRPORTS ON COVID-19 RATES IN EUROPEAN REGIONS
Authors: Stanton-Geddes Michael 
Published: April 2022

This study provides the result of statistical analysis of weekly airport passenger traffic data and the rate of new COVID-19 cases (COVID-19 incidence rate) in Europe at both the country and sub national level during 2020, controlling for the prior incidence rate, the level of stringency of government measures, and the mobility of people. This paper focuses on the relationship between air travel and the COVID-19 incidence rate during the second half of the year because this addresses the real problem faced in Europe about whether to permit air travel after a novel virus was already highly present in the community. The paper does not seek to add to the literature about the role of air travel in the initial propagation of a novel virus, nor does it consider the impact of vaccine availability.
The analysis provides evidence that a 10% increase in airport passengers is correlated with a 0.14% increase in the COVID-19 incidence rate in Europe’s subnational regions during the second half of 2020. As comparison, an increase of 10% in the index of the stringency of government measures is correlated with a 4.3% reduction in the COVID-19 rate, and 10% more mobility in the population with a 2.9% increase in the COVID-19 rate. The paper uses a model that is based in literature and applies ordinary least squares (OLS) regression techniques for fixed effects and pooled panel data.
The finding that increases in air transport traffic when a novel virus is already widely present suggests that efforts to restrict or control air travel are not likely to be efficient.
Deskripsi Pemahaman Siswa terhadap Kedisiplinan sebagai Penanaman Nilai-Nilai Kristen
Authors: Nuban Imanuel , Triposa Reni , Arifianto Yonatan Alex 
Published: April 2022

Kedisiplinan dalam konteks pendidikan pada hakikatnya merupakan bagian dari pendidikan karena kedisiplinan merupakan suatu proses yang dibiasakan dan di terapkan dalam kehidupan sehari-hari, seperti norma-norma, sikap, serta aturan yang dianggap menjadi tolak ukur dalam sekolah. Disiplin adalah bukan untuk membebani siswa agar peserta didik terbebani, tetapi setiap siswa punya disiplin dalam hidupnya, bahkan setiap kelompok dalam belajar mengajar akan ada disiplin karakter untuk menaati aturan-aturan yang ada, jika siswa dapat memahami arti disiplin maka siswa akan senang dan mau menataati setiap peraturan-peraturan yang ada. Mengunakan metode kualitatif deskriptif dengan pendekatan studi literature maka dapat disimpulkan bahwa penanaman nilai kristen bertitik tolak pada peran guru dalam menanamkan disiplin belajar dalam proses pembelajaran yang konduksif di sekolah, sebab guru adalah salah satu diantara faktor pendidikan yang memiliki peranan paling strategis. Peran guru dalam pembentukan disiplin belajar peserta didik, sebagai pembimbing yang menuntun peserta didik dengan memberikan dukungan dan arahan, peran guru sebagai contoh teladan disiplin yang dapat siswa menjadikan idola peserta didik, dan mengembangkan ilmu bagi kemajuan belajar siswa.