bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of 19 years of restoration managements on forage quality and herbage‐soil relationships within improved upland grassland
Authors: Kajzrová Klára , Kassahun Teowdroes , Pavlů Lenka , Pavlů Vilém V. , Fraser Mariecia D. 
Published: July 2022

AbstractRestoration managements based on extensification strategies are commonly used to improve biodiversity on formerly improved grasslands. In this study the long‐term effects of six different restoration management regimes (extensive sheep grazing only, hay cutting only, hay cutting followed by aftermath extensive grazing; each with and without lime application) on forage quality, and soil/herbage/sward characteristics relationships were determined and compared with a conventionally fertilized, limed and extensively grazed control.Restoration managements incorporating cutting resulted in higher forage quality than forage from grazed‐only treatments; the latter featuring only a few forb species and large proportion of ungrazed matured grasses plus dead biomass. Concentrations of crude protein, neutral detergent fibre and K in the herbage were negatively correlated with cover of forbs and total number of plant species, whereas in vitro organic matter digestibility, and concentrations of water‐soluble carbohydrates, Ca, Mg, and Na were correlated positively. In contrast, concentrations of Ca, Mg, and Na were negatively correlated with the total cover of graminoids and dry matter standing biomass. Regardless of management treatment the forage was generally suitable only for sheep or beef cattle feeding. A positive relationship between P and K concentration in the soil and in the herbage was recorded. No effect of previous liming on forage quality was found. Overall, this study found introducing long‐term restoration managements to support biodiversity by postponing the timing of the first defoliation by cutting to mid growing season did not deteriorate forage quality in comparison with continual extensive sheep grazing in improved upland grassland.
Downregulation of hsa‐miR‐548d‐3p and overexpression of HOXA9 in diffuse large B‐cell lymphoma patients and the risk of R‐CHOP chemotherapy resistance and disease progression
Authors: Ting Choo‐Yuen , Tan Soo‐Yong , Gan Gin‐Gin , Zain Shamsul‐Mohd , Pung Yuh‐Fen , Ong Diana Bee‐Lan , Bee Ping‐Chong 
Published: July 2022

AbstractIntroductionRoutine categorization of DLBCL patients into GCB and non‐GCB groups by Hans' criteria could not accurately predict chemotherapy resistance and disease progression in patients treated with standard R‐CHOP therapy. There is a need to identify better biomarker predictors to enhance assisted selection of chemotherapy regimens for DLBCL patients.Aim of the studyTo identify dysregulated miRNAs and mRNAs that are predictive of resistance to R‐CHOP chemotherapy or disease progression in patients with DLBCL.MethodsmiRNA and mRNA profiling were performed on archival FFPE samples of the DLBCL patients. miRabel and miRNet bioinformatic tools were applied to determine experimental validated miRNA‐mRNA target interaction. The significance of the genomic predictive values was assessed using adjusted odds ratios (AOR) and 95% confidence intervals (CI).Results19/36 were R‐CHOP therapy‐resistant whilst 17/36 were R‐CHOP therapy‐sensitive. Ten dysregulated miRNAs and 12 dysregulated mRNAs were identified in therapy‐resistant DLBCL patients. These dysregulated miRNAs and mRNA cause therapy resistance and disease progression in DLBCL patients, most likely via upregulation of the anti‐apoptotic protein bcl2, activation of the JAK/STAT signalling pathway and dysregulation of p53 pathway. Downregulation of hsa‐miR‐548d‐3p and overexpression of HOXA9 mRNA were significantly associated with therapy resistance and disease progression in DLBCL patients [hsa‐miR‐548d‐3p AOR: 0.258, 95%CI: 0.097–0.684, p = 0.006].ConclusionDLBCL patients with downregulation of hsa‐miR‐548d‐3p and overexpression of HOXA9 mRNA are more likely to experience R‐CHOP therapy resistance and disease progression.
Cuticle protein gene LmCP8 is involved in the structural development of the ovipositor in the migratory locust Locusta migratoria
Authors: Zhao Xiaoming , Su Yazhi , Shao Ti , Fan Zhiyan , Cao Lili , Liu Weimin , Zhang Jianzhen 
Published: July 2022

AbstractThe ovipositor comprises the external genitalia of female insects, which plays an important role in the mating and ovipositing process of insects. However, it remains rudimentary of regional gene expression and physiological function in the ovipositor during structural development. Here, we analysed the basic structure and characteristics of the ovipositor in the migratory locust Locusta migratoria. RNA‐seq analysis revealed the specialization of chitin metabolism, lipids synthesis and transport, tanning and cuticular protein genes in the ovipositor. Among them, two cuticle protein genes, LmCP8 and LmACP79, were identified, which are specifically expressed in the ovipositor. Functional analysis based on RNA interference showed that deficiency of LmCP8 affected the structural development of the ovipositor resulting in the retention of a large number of remaining unproduced oocysts in the ovary of the locusts. Our results provide a fundamental resource to investigate the structural development and physiological function of the ovipositor in L. migratoria.
Supplier selection and contract enforcement: Evidence from performance bonding
Authors: Giuffrida Leonardo M. , Rovigatti Gabriele 
Published: July 2022

AbstractWe analyze an important but little‐studied institution for balancing supply risk in the management of procurement operations: performance bonding. By adding the surety as a third party that guarantees contract fulfillment between supplier and buyer, performance bonding aims to streamline the purchasing process by influencing both contractor selection in the bidding phase and contract enforcement during project execution. Using the data on US government procurement from 2005 to 2015 and exploiting an exogenous variation in the threshold for its application to construction contracts, we find that performance bonding improves contract outcomes by 10.5% and 3.7% in terms of delays and extra costs, respectively. Net of bond premia, which by law are included in the award amounts, this effect translates into savings of about 4% in the budget for federal construction projects and 16% for mid‐size projects. We provide suggestive evidence on the effectiveness of selection and monitoring by sureties as driving channels.
A systematic review and meta‐analysis of the relationship between farm management, water quality and pathogen outbreaks in tilapia culture
Authors: Paredes‐Trujillo Amelia , Mendoza‐Carranza Manuel 
Published: July 2022

AbstractA systematic review and meta‐analysis of on farm management and water quality as risk factors triggering pathogen outbreaks in tilapia culture is presented. A total of 121 papers from 28 countries, 37 devoted to management and 84 to water quality associated with the presence of pathogens in tilapia culture were analysed. Most of research has been made on ponds, focusing on bacteria and metazoans. Meta‐analysis reveals the proportion of research showing statistical inferences between management (34%), water quality (38% for bacteria and 16.2% for metazoans) and pathogen outbreaks. The most cited parameters related to pathogens outbreaks were temperature (>30°C), dissolved oxygen (<5.0 mg/L), pH (<8.0) and ammonia (1.0 >mg/L) 65, 38, 32 and 34 papers, respectively. However, statistical inference was <20%. Meta‐analysis reveals tendencies between high temperatures (30–35°C), low dissolved oxygen (<5 mg/L) and high NH3(1–5 mg/L) with pathogen outbreaks. Despite the knowledge about the relationship between management and water quality as factors for pathogen effects, most information is descriptive and empirical. Future research on tilapia culture outbreaks should be focused on the effects of multiple stressors affecting tilapia pathogen outbreaks, thereby generating strategies to prevent diseases and financial losses.
Developing the Quality in Nutrition Care Model for Dietitians (QUINCE‐MOD) in primary care: A mixed‐method survey of healthcare consumers and professionals
Authors: Kirkegaard Amy , Mitchell Lana , Ball Lauren , Williams Lauren T. 
Published: July 2022

AbstractBackgroundEvidence‐based models are effective tools for informing quality improvement activities in health care. The Quality in Nutrition Care (QUINCE) model is an evidence‐based model that describes quality nutrition care in the primary care setting but has not been applied to primary care dietetics. The present study comprised two phases. In the first phase, the study aimed to evaluate and compare healthcare consumers and professional perspectives on the importance of the 10 statements to understand their relevance for dietetics care. In the second phase, the study aimed to test and enhance the QUINCE model using healthcare consumer and professional responses to statements that were identified as relevant to primary care dietetics in the first phase.MethodsQuantitative and qualitative data were collected using an online survey comprising statements describing quality dietetic care adapted from the complexity literature. Quantitative data were analysed using descriptive statistical analysis to investigate statement importance as rated by participants using a six‐point Likert scale. Qualitative content analysis was performed on the qualitative data using a deductive‐inductive approach to identify patterns in the data. The QUINCE model was compared with identified patterns and modified as appropriate.ResultsOne hundred and fourteen consumers and 107 primary care professionals agreed on the importance of seven statements but expressed divergent views on the remaining three statements that addressed: (1) access to medical records; (2) access to performance and safety information; and (3) variation in care between dietitians. The QUINCE model was modified to produce the Quality in Nutrition Care Model for Dietitians (QUINCE‐MOD) describing quality dietetic care in the primary care setting.ConclusionsConsumers and professionals share views on some aspects of quality dietetic care but diverge on other aspects. The QUINCE‐MOD is an evidence‐based, complexity‐informed model that describes components of quality relevant to primary care dietetics. The model should be applied at the practice level to reflect the unique perspective of quality at that level.
Microbiological quality of hospital‐prepared blenderised tube feeding
Authors: Adibi Shiva , Afshari Asma , Norouzy Abdolreza , Nematy Mohsen , Ehsani Ali , Hashemi Mohammad 
Published: July 2022

AbstractBackgroundContaminated blenderised tube feeding (BTF) causes numerous infections in patients with deficient immune systems. The microbial quality of BTF should be thoroughly monitored to reduce the risks of microbial agents and prevent food safety problems such as food poisoning and food‐borne illnesses. The aim of this study was to survey the contamination rate of BTF samples prepared in the teaching hospitals in Mashhad, Iran.MethodsThis study was conducted on 24 samples of BTF prepared in four teaching hospitals in Mashhad city; the samples were collected randomly. Then specific culture media were used for detected and counted Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, Clostridium perfringens, Bacillus cereus, coliforms and Escherichia coli. The final confirmation of the isolates was performed using polymerase chain reaction.ResultsThe total bacterial count was determined in the BTF samples and compared with the Food and Drug Administration medical food standards; 91.6% of the samples had 5.2 ± 0.1 log CFU/ml microbial bacterial contamination considering the standard range. The mean prevalence of contamination in these samples was measured for coliforms 4.9 ± 0.17 log CFU/ml, B. cereus 3.6 ± 0.16 log CFU/ml, S. aureus 3.7 ± 0.15 log CFU/ml and C. perfringens 4.7 ± 0.08 log CFU/ml (p < 0.05). Moreover, E. coli 11 (45.8%), Salmonella spp. 9 (37.5%) and L. monocytogenes 17 (70.8%) samples were detected.ConclusionGiven the high consumption of BTF and the transmission of food contamination to hospitalised patients, it is essential to improve the hygienic conditions at the site of BTF preparation to prevent re‐contamination.
Illuminating associations between parenting and deleterious neighborhood characteristics via exhaustive modeling
Authors: Burt S. Alexandra , Thaler Daniel , Shewark Elizabeth A. , Pearson Amber L. , Anaya Carolina , Tomlinson Rachel C. , Neiderhiser Jenae M. , Klump Kelly L. , Lonstein Joseph S. 
Published: July 2022

AbstractObjectiveOur goal was to illuminate associations between specific characteristics of under‐resourced neighborhoods (i.e., socioeconomic deprivation, danger) and specific aspects of parenting (e.g., parental praise, parental nurturance, harsh parenting, and parental control).BackgroundPrior work has highlighted associations between level of neighborhood disadvantage and the parenting of its residents. However, this work has yet to clarify the specific characteristics of the neighborhood or the types of parenting involved.MethodExhaustive modeling analyses were conducted in a sample of 1030 families of twins (average age 8 years; 51% male, 49% female; the racial composition was 82% White, 10% Black, 1% Asian, 1% Indigenous, 6% multiracial) from the Twin Study of Behavioral and Emotional Development in Children. Neighborhood and parenting were assessed using multiple informants and assessment strategies (neighborhood informants, family informants, administrative data, and videotaped parent–child interactions).ResultsNeighborhood socioeconomic deprivation (i.e., limited institutional and economic structural resources) demonstrated small but consistent negative associations with positive parenting behaviors and maternal control, but not with negative parenting behaviors. Neighborhood danger (i.e., recorded crime, fear of crime, exposure to community violence), by contrast, demonstrated weaker associations with parenting that dissipated once we controlled for overlap with socioeconomic deprivation.ConclusionDanger and socioeconomic deprivation do not function as interchangeable characteristics of under‐resourced neighborhoods, at least in terms of their association with positive parenting. Future studies should identify the specific mechanisms through which neighborhood socioeconomic deprivation is associated with less nurturing parenting.
SPP1 as a key gene in the lymph node metastasis and a potential predictor of poor prognosis in head and neck carcinoma
Authors: Feng Shunli , Yuan Wubin , Sun Zhen , Guo Xiaopeng , Ling Junjun , Chang Aoshuang , Zhao Houyu , Zhuo Xianlu 
Published: July 2022

AbstractBackgroundLymph node metastasis (LNM) is an important cause leading to recurrence and development of head and neck carcinoma (HNC), with the precise mechanisms unclear. Thus, we aimed to identify the key genes involved in LNM and further evaluate their expressions and roles.MethodsA cohort of HNC in the TCGA was analyzed. The study involved three phases (one screening and two validation phases). First, the differentially expressed genes regarding LNM were screened, from which a key gene was identified by a series of data mining approaches. Then, the expressions and roles of the key gene were validated in HNC through bioinformatics. Afterward, the expression of the key gene was detected by qPCR, western blot, and Immunohistochemistry based on a cell model and a tissue microarray. Further, colony formation and transwell migration and invasion assays were used to evaluate the roles of the key gene.ResultsSPP1 was overexpressed in HNC tissues and was identified as the key gene. Overexpression of SPP1 in HNC was correlated with advanced pathological stages and T‐stage, as well as the presence of LNM, which predicted poor prognosis. The expression of SPP1 was closely associated with the infiltration of immune cells in HNC, especially M2 macrophages. Lab experiments confirmed that SPP1 silence in HNC cells resulted in weakened invasive and metastatic abilities.ConclusionThis study reveals that SPP1 may be a key gene associated with LNM in HNC, raising the possibility of SPP1 as a target for HNC prevention and treatment.
Demographic, physiological and genetic factors linked to the poleward range expansion of the snail Nerita yoldii along the shoreline of China
Authors: Wang Jie , Cheng Zhi‐Yuan , Dong Yun‐Wei 
Published: July 2022

AbstractSpecies range shift is one of the most significant consequences of climate change in the Anthropocene. A comprehensive study, including demographic, physiological and genetic factors linked to poleward range expansion, is crucial for understanding how the expanding population occupies the new habitat. In the present study, we investigated the demographic, physiological and genetic features of the intertidal gastropod Nerita yoldii, which has extended its northern limit by ~200 km over the former biogeographical break of the Yangtze River Estuary in recent decades. Data from neutral single nucleotide polymorphisms (SNPs) showed that the new marginal populations formed a distinct cluster established by a few founders. Demographic modelling analysis revealed that the new marginal populations experienced a strong genetic bottleneck followed by recent demographic expansion. Successful expansion that overcame the founder effect might be attributed to its high capacity for rapid population growth and multiple introductions. According to the non‐neutral SNPs under diversifying selection, there were high levels of heterozygosity in the new marginal populations, which might be beneficial for adapting to the novel thermal conditions. The common garden experiment showed that the new marginal populations have evolved divergent transcriptomic and physiological responses to heat stress, allowing them to occupy and survive in the novel environment. Lower transcriptional plasticity was observed in the new marginal populations. These results suggest a new biogeographical pattern of N. yoldii has formed with the occurrence of demographic, physiological and genetic changes, and emphasize the roles of adaptation of marginal populations during range expansion.
A new cocktail formula with diprospan of local infiltration analgesia in primary total hip arthroplasty: A prospective, randomized, controlled, observer‐blinded study
Authors: Wang Hao‐Yang , Xiao Qiang , Luo Zhen‐Yu , Pei Fu‐Xing , Wang Duan , Zhou Zong‐Ke 
Published: July 2022

ObjectiveThis study aimed to observe the analgesic effect of the cocktail formulation with diprospan during total hip arthroplasty (THA).MethodsFrom September 2018 to April 2019, 120 patients undergoing primary unilateral THA were included in this prospective, randomized, observer‐blinded study. Patients were randomized into three groups, according to the different local infiltration analgesia (LIA) strategies: LIA with ropivacaine (the ropivacaine group, n = 40), LIA with a new cocktail containing ropivacaine, diprospan, and morphine (the cocktail group, n = 40), and the control group (n = 40). The primary outcomes included postoperative pain scores. The resting visual analogue scale (VAS) scores were measured at 2, 6, and 12 h after the surgery (a.m. and p.m.) on postoperative day (POD) 1, POD2, and the day of discharge. Movement VAS scores were assessed at 6 h, 12 h after the operation (a.m. and p.m.) on POD1, POD2, and the day of discharge. The secondary outcomes included opioid consumption, postoperative hospital stay, range of motion of the hip at discharge, patient satisfaction, and the results of the follow‐up.ResultsAfter the screening, 120 patients were randomized into three groups (40 patients in each group). All of the patients completed the trial. The resting VAS scores in the ropivacaine group and cocktail group at 2 h were lower than those in the control group (P < 0.001 and P < 0.001, respectively, F = 17.054), and the same trend was also postoperatively found at 6 h (p = 0.005 and P = 0.002, F = 6.212). Twelve hours after the operation, the pain score in the cocktail group was lower than that in the other two groups, but only the difference between the cocktail group and the control group was statistically significant (P = 0.018, F = 3.144). From the morning of the first postoperative day to the a.m. on POD 2, the VAS scores in the cocktail group were significantly lower than those in the ropivacaine group and the control group. Furthermore, the movement VAS scores in the ropivacaine group and the cocktail group were better than those in the control group at 6 and 12 h post‐operation (P < 0.05). The per capita opioid consumption in the cocktail group was less than that in the ropivacaine group and the control group within 24 h post‐operation. There were no significant differences in the comparison of additional indicators among the three groups.ConclusionThe new cocktail with diprospan had a better result and longer duration time for early postoperative pain control in primary THA via the posterolateral approach under general anesthesia, especially for treating resting pain.
Unexpected large device related thrombus at 12 months follow‐up after left atrial appendage closure
Authors: Urbanek Lukas , Chen Shaojie , Bordignon Stefano , Tohoku Shota , Bologna Fabrizio , Schulte‐Hahn Britta , Chun KR Julian , Schmidt Boris 
Published: July 2022

AbstractAn 89‐year‐old woman underwent left atrial appendage (LAA) closure (LAAC) in our hospital because of recurrent gastrointestinal bleedings. The first transesophageal echocardiography (TEE) follow‐up at six weeks revealed a complete sealing of the LAA and no device related thrombus. In a TEE follow‐up at one year after the LAA closure, a large device related thrombus (6 × 3 cm) was found. Treated with oral anticoagulation (apixaban) the thrombus showed a partial resolution one year later.
Image analysis systems to calculate the surface area of vitiligo lesions: A systematic review of measurement properties
Authors: van Geel Nanja , Saeys Imke , Van Causenbroeck Jérôme , Duponselle Jolien , Grine Lynda , Pauwels Nele , Hilhorst Niels , Herbelet Sandrine , Ezzedine Khaled , Speeckaert Reinhart 
Published: July 2022

AbstractSeveral digital image analysis systems have been developed for surface calculation of vitiligo lesions. Critical assessment of their measurement properties is crucial to support evidence‐based recommendations on the most suitable instruments and will reveal the need for future research. A systematic review was performed to systematically summarize, compare, and critically assess the measurement properties of digital and analogue analysis systems for surface calculation of vitiligo lesions following the Consensus‐Based Standards for the Selection of Health Measurement Instruments (COSMIN) recommendations. Nineteen clinical trials were selected including 25 different instruments. Manual tracing on transparent sheets (contact planimetry) combined with digital measurement or point counting can be considered as the best validated method for the evaluation of target lesions taking into account the skin curvatures. Two‐dimensional digital imaging analysis on photographs seems also robust although confirmatory data of different research groups using the same digital instrument in a wide range of skin types are missing. Analysis based on 3D photography is still in its early stage but is promising for whole‐body analysis. However, the reported data on the quality of the instruments for surface area calculation of vitiligo lesions were in general rather limited. Therefore, future high‐quality validation studies are required also including full body evaluations.