bluuebunny/crossref_metadata_2025_split huggingface dataset:
GADOLINIUM(III) COMPLEX OXIDE COMPOUNDS OF OBTAINED FROM MOLYBDATE-PHOSPHATE MOLTEN SALT
Authors: Terebilenko Kateryna , Zozuliia Valeriia , Tokmenko Inna , Slobodyanik Mykola 
Published: October 2022

The peculiarities of K2Gd(PO4)(MoO4) single crystal growth from combined phosphate-molybdate melts have been investigated. The formation regions of GdPO4, KGd(MoO4)2 and their cocrystallization fields with the mixed anionic compound potassium – gadolinium have been established. IR spect­roscopy showed that in all compounds molybdenum and phosphorus are in a tetrahedral environment, and the phase composition was confirmed by X-ray powder. Crystallization of the target compound K2Gd(PO4)(MoO4) is observed in the pseudobinary section K2Mo2O7-KPO3 orthiorombic system, space group Ibca, Z = 8, а = 19,694(4) Å, b = 12,260(3) Å, c = 6,961(3) Å. Double phosphate K3Gd(PO4)2 crystallizes in the form of colorless prismatic crystals from the melts with the initial ratio K/P = 1.7–3.3, which practically corresponds to the section K2MoO4 – K4P2O7. Phosphate GdPO4 crystallizes in the form of colorless prismatic crystals up to 0.1 mm in size at the maximum KPO3 content in the melt, which corresponds to K/P ratio = 0.20–0.50 and K/(Mo+P) = 0.25–0.33. Crystal-chemical criteria for the formation of mixed-anion-type layered frameworks, island structures, and three-dimensional frameworks based on mixed phosphate-molybdate systems and rare earth elements are proposed. The key factor in the formation of complex oxide compounds of rare earth elements from combined molybdate-phosphate melts is the ratio of K/Mo in the initial solution - melt: 1) at K/Mo = 0.5–1.1, LnPO4 orthophosphates are formed, which are characterized by a 3D framework on the basis of LnO7/LnO8poly­hedra condensed into a three-dimensional framework; at K/Mo = 1.2–1.5, mixed anionic compounds of the composition K2Ln(PO4)(MoO4) (Ln – Gd- Tb) with a layered structure are formed, where LnО8 forms zigzag chains; 3) at K/Mo = 1.6–3.0, double orthophosphates of the composition K3Ln(PO4)2 with an island structure are formed.
Association of virginiamycin and multiple supplement for cattle fed a high-quality tropical forage
Authors: Vidal Raphaela C. , Lima Nicole S.A. , Sampaio Claudia B. , Duarte Marcio S. , Detmann Edenio 
Published: October 2022

The aim of this study was to evaluate the effect of adding virginiamycin to either mineral mixture or multiple supplement on intake, digestion, ruminal fermentation profile, rumen microbial production, blood metabolites, and liver metabolism of zebu heifers fed a high-quality tropical forage. Eight Brahman heifers were assigned to a replicated 4 × 4 Latin Square design. The treatments were: mineral mixture, mineral mixture with virginiamycin, multiple supplement, and multiple supplement with virginiamycin. The basal diet consisted of a high-quality Tifton 85 hay (Cynodom sp.) chopped at 10-cm particle size and fed twice daily. The mineral mixture was provided daily at 120 g/animal. The multiple supplement was formulated to provide 300 g of crude protein (CP)/kg as fed, contained mineral mixture, corn grain, and urea: ammonium sulfate, and was daily provided at 200 g/animal. The mineral mixture and multiple supplement provided the same daily amount of minerals. The amount of supplemental virginiamycin was based on a maximum theoretical response on animal performance (50 mg/100 kg body weight) and daily mixed to the supplements types. The treatments were compared according to a 2 × 2 factorial arrangement (mineral mixture and multiple supplement, with or without virginiamycin). The virginiamycin supplementation did not alter either voluntary intake or digestibility (P≥0.44). Using the multiple supplement decreased forage (P&lt;0.05) and digested organic matter (DOM, P&lt;0.10) intake, but increased dietary CP : DOM ratio (P&lt;0.01). The multiple supplement increased the ruminal ammonia concentration (P&lt;0.04) and the acetate-to-propionate ratio (P&lt;0.06). Virginiamycin increased urinary nitrogen (N) when provided along with multiple supplement (P&lt;0.05). Fecal N was decreased by multiple supplement (P&lt;0.06). Despite these effects, no effect was verified on either body N accretion (P≥0.48) or microbial N production in the rumen (P≥0.27). Virginiamycin decreased the blood IGF-1 (P&lt;0.07). The hepatic gene expression for propionyl-CoA carboxylase was increased by virginiamycin (P&lt;0.01). Virginiamycin increased the hepatic gene expression of both citrate synthase and pyrivate carboxylase when mineral mixture was provided (P&lt;0.01), but decreased it when animals were fed multiple supplement (P&lt;0.04). Using a low-intake multiple supplement with a high CP content for cattle fed high-quality forage causes a substitutive effect on forage intake, but keeps nitrogen accretion unchanged. That pattern indicates an improvement in feed efficiency. On the other hand, virginiamycin supplementation seems to cause some post-prandial influences, which may vary according to the type of supplement. Those influences apparently improve animal efficiency.
Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells
Authors: Colombini Alessandra , Libonati Francesca , Cangelosi Davide , Lopa Silvia , De Luca Paola , Coviello Domenico Antonio , Moretti Matteo , de Girolamo Laura 
Published: October 2022

Inflammatory processes contribute to osteoarthritis (OA) severity and progression. Mesenchymal stem cells, particularly those derived from adipose tissue (ASCs), are able to sense and control the inflammatory environment. This immunomodulatory potential can be boosted by different priming strategies based on inflammatory stimulation. The aim of the present study is to investigate the transcriptional modulation of a huge panel of genes and functionally verify the predicted immunomodulatory ability of ASCs after interleukin one beta (IL-1β) priming. ASCs were isolated from adipose tissue obtained from three donors and expanded. After stimulation with 1 ng/ml of IL-1β for 48 h, cells were collected for gene array and functional tests. Pooled cells from three donors were used for RNA extraction and gene array analysis. Gene Ontology (GO) enrichment analysis and Gene Set Enrichment Analysis (GSEA) were performed to assess the involvement of the modulated genes after priming in specific biological processes and pathways. Functional co-culture tests of ASCs with T cells and macrophages were performed to assess the ability of primed ASCs to modulate immune cell phenotype. Among the overall genes analyzed in the gene array, about the 18% were up- or down-regulated in ASCs after IL-1β priming. GO enrichment analysis of up- or down-regulated genes in ASCs after IL-1β priming allowed identifying specific pathways involved in the modulation of inflammation and extracellular matrix remodeling. The main processes enriched according to the GSEA are related to the inflammatory response and cell proliferative processes. Functional tests on immune cells showed that primed and non-primed ASCs induced a decrease in the CD3+ T lymphocytes survival rate and an anti-inflammatory macrophage polarization. In conclusion, IL-1β priming represents a tailored strategy to enhance the ability of ASCs to direct macrophages towards an anti-inflammatory phenotype and, consequently, improve the efficacy of ASCs in counteracting the OA inflammatory component.
The targeting imaging and treatment capacity of gelsolin-targeted and paclitaxel-loaded PLGA nanoparticles in vitro and in vivo
Authors: Ji Jiamei , Qin Haocheng , Yang Yan , Wu Jun , Wu Juan 
Published: October 2022

As a vital sign of carcinomas, lymph node metastasis is closely related to poor prognosis due to a lack of identification and effective treatment in the early stage. Nanoscale contrast agents targeting specific tumor antigens are expected to identify tumor metastasis in the early stage and achieve precise treatment. As a biomarker in the early stage of tumor invasion and metastasis, gelsolin (GSN) might be a promising molecular target to identify and screen tumor metastasis through the lymphatic system. Therefore, GSN-targeted paclitaxel-loaded poly(lactic-co-glycolic acid) nanoparticles (GSN-PTX-PLGA NPs) were prepared, and their physicochemical properties, encapsulation efficiency, drug loading, and dissolution were determined. Besides, uptake experiments and the fluorescent imaging system were used to evaluate their targeting capability. The targeting imaging and treatment capacity were also assessed by experiments in vitro and in vivo. The diameter of the GSN-PTX-PLGA NPs was 328.59 ± 3.82 nm. Hca-F cells with GSN-PLGA NPs showed stronger green fluorescence than Hca-P cells. DiI-labeled GSN-PLGA NPs in tumor-bearing mice and isolated organs exhibited more prominent fluorescence aggregation. The imaging of GSN-PLGA NPs was satisfactory in vitro, and the echo intensity gradually increased with increasing concentrations of GSN-PLGA NPs. After treatment with GSN-PTX-PLGA NPs, there was an obvious decrease in tumor volume and lymph node metastasis rate compared to the other groups (p &lt; 0.05). In conclusion, GSN-PTX-PLGA NPs have a remarkable targeting capacity in vivo and in vitro, and they effectively inhibit tumor growth and lymph node metastasis in vivo.
Luminescent quantum dot films improve light use efficiency and crop quality in greenhouse horticulture
Authors: Hebert Damon , Boonekamp Jeroen , Parrish Charles H. , Ramasamy Karthik , Makarov Nikolay S. , Castañeda Chloe , Schuddebeurs Lisanne , McDaniel Hunter , Bergren Matthew R. 
Published: October 2022

The spectral quality of sunlight reaching plants remains a path for optimization in greenhouse cultivation. Quantum dots represent a novel, emission-tunable luminescent material for optimizing the sunlight spectrum in greenhouses with minimal intensity loss, ultimately enabling improved light use efficiency of plant growth without requiring electricity. In this study, greenhouse films containing CuInS2/ZnS quantum dots were utilized to absorb and convert ultraviolet and blue photons from sunlight to a photoluminescent emission centered at 600 nm. To analyze the effects of the quantum dot film spectrum on plant production, a 25-week tomato trial was conducted in Dutch glass greenhouses. Plants under the quantum dot film experienced a 14% reduction in overall daily light integral, resulting from perpendicular photosynthetically active radiation transmission of 85.3%, mainly due to reflection losses. Despite this reduction in intensity, the modified sunlight spectrum and light diffusion provided by the quantum dot film gave rise to 5.7% improved saleable production yield, nearly identical total fruiting biomass production, 23% higher light use efficiency (g/mol), 10% faster vegetative growth rate, and 36% reduced tomato waste compared to the control, which had no additional films. Based on this result, materials incorporating quantum dots show promise in enabling passive, electricity-free spectrum modification for improving crop production in greenhouse cultivation, but extensive controlled crop studies are needed to further validate their effectiveness.
Assessment of the causal association between celiac disease and cardiovascular diseases
Authors: Huang Jian 
Published: October 2022

BackgroundEpidemiological studies have reported inconsistent results of the association between celiac disease (CD) and cardiovascular diseases. Moreover, the causality remains largely unknown. Therefore, we aimed to investigate whether CD is causally associated cardiovascular diseases, including ischemic stroke, large artery stroke, cardioembolic stroke, small vessel stroke, coronary heart disease, myocardial infarction, angina, heart failure, atrial fibrillation, and venous thromboembolism using an mendelian randomization (MR) approach.MethodsSummary-level data for CD were derived from a large-sample genome-wide association study (GWAS) including 12,041 CD cases and 12,228 controls of European ancestry. The corresponding data for ischemic stroke (34,217 cases and 406,111 controls), large artery stroke (4,373 cases and 406,111 controls), cardioembolic stroke (7,193 cases and 406,111 controls), small vessel stroke (5,386 cases and 192,662 controls), coronary heart disease (22,233 cases and 64,762 controls), myocardial infarction (11,622 cases and 187,840 controls), angina (18,168 cases and 187,840 controls), heart failure (47,309 cases and 930,014 controls), atrial fibrillation (60,620 cases and 970,216 controls), and venous thromboembolism (9,176 cases and 209,616 controls) were obtained from the IEU GWAS database. We calculated the causal effect using the inverse variance weighted method. Sensitivity analyses and leave-one-out analyses were performed to ensure the consistency and robustness of causal estimates.ResultsThe MR inverse variance weighted estimates indicated no causal effect of genetically predicted CD on ischemic stroke (OR = 1.001, 95% CI: 0.984-1.018), large artery stroke (OR = 1.003, 95% CI: 0.961-1.048), cardioembolic stroke (OR = 1.009, 95% CI: 0.977-1.042), small vessel stroke (OR = 1.023, 95% CI: 0.981-1.066), coronary heart disease (OR = 0.995, 95% CI: 0.977-1.013), myocardial infarction (OR = 0.994, 95% CI: 0.959-1.030), angina (OR = 1.006, 95% CI: 0.981-1.032), heart failure (OR = 0.999, 95% CI: 0.982-1.016), atrial fibrillation (OR = 1.000, 95% CI: 0.990-1.011), and venous thromboembolism (OR = 1.001, 95% CI: 0.971-1.032). Sensitivity analyses using the MR-Egger, weighted median, and simple mode methods yielded similar results. No evidence of horizontal pleiotropy was identified (MR Pleiotropy Residual Sum and Outlier global test and MR-Egger intercept with P &gt; 0.05).ConclusionOur findings do not support a causal contribution of CD itself to ischemic stroke, large artery stroke, cardioembolic stroke, small vessel stroke, coronary heart disease, myocardial infarction, angina, heart failure, atrial fibrillation, and venous thromboembolism risk.
Research status and trends of the diabetic cardiomyopathy in the past 10 years (2012–2021): A bibliometric analysis
Authors: Wang Sicheng , Tian Chuanxi , Gao Zezheng , Zhang Boxun , Zhao Linhua 
Published: October 2022

BackgroundDiabetic cardiomyopathy is one of the most life-threatening diabetic complications. However, the previous studies only discuss a particular aspect or characteristic of DCM, the current state and trends were explored by limited research. We aimed to perform a systemically bibliometric study of DCM research progress status in the past decade, visualize the internal conceptual structure and potential associations, and further explore the prospective study trends.MethodsArticles related to DCM published from January 2012 to December 2021 were collected in the Web of Science core collection (WoSCC) database on June 24, 2022. We exported all bibliographic records, including titles, abstracts, keywords, authorship, institutions, addresses, publishing sources, references, citation times, and year of publication. In addition, the journal Impact Factor and Hirsch index were obtained from the Journal Citation Report. We conducted the data screening, statistical analysis, and visualization via the Bibliometrix R package. VOS viewer software was employed to generate the collaboration network map among countries and institutions for better performance in visualization.ResultsIn total, 1,887 original research articles from 2012 to 2021 were identified. The number of annual publications rapidly increased from 107 to 278, and a drastic increase in citation times was observed in 2017–2019. As for global contributions, the United States was the most influential country with the highest international collaboration, while China was the most productive country. Professor Cai Lu was the most prolific author. Shandong University published the most articles. Cardiovascular Diabetology journal released the most DCM-related articles. “Metabolic Stress-induced Activation of FoxO1 Triggers Diabetic Cardiomyopathy in Mice” Battiprolu PK et al., J Clin Invest, 2012. was the most top-cited article regarding local citations. The top three keywords in terms of frequency were apoptosis, oxidative stress, and fibrosis. The analysis of future topic trends indicated that “Forkhead box protein O1,” “Heart failure with preserved ejection fraction,” “Dapagliflozin,” “Thioredoxin,” “Mitochondria dysfunction,” “Glucose,” “Pyroptosis,” “Cardiac fibroblast” and “Long non-coding RNA” could be promising hotspots.ConclusionThis study provides meaningful insights into DCM, which is expected to assist cardiologists and endocrinologists in exploring frontiers and future research directions in the domain through a refined and concise summary.
Levosimendan in acute heart failure with severely reduced kidney function, a propensity score matched registry study
Authors: Rottmann Felix Arne , Breiden Ann Katrin , Bemtgen Xavier , Welte Thomas , Supady Alexander , Wengenmayer Tobias , Staudacher Dawid Leander 
Published: October 2022

BackgroundPatients with heart failure frequently present with kidney dysfunction. Kidney function is relevant, as prognosis declines with reduced kidney function and potentially beneficial drugs like levosimendan are contraindicated for missing safety data.Materials and methodsA single-center retrospective registry study was conducted including all patients receiving levosimendan on a medical intensive care unit between January 2010 and December 2019. Exclusion criteria were a follow-up less than 24 h or missing glomerular filtration rate (eGFR) before administration of levosimendan. The first course of treatment was evaluated. Patients were stratified by eGFR before drug administration and the primary endpoint was a composite of supraventricular-, ventricular tachycardia and death within 7 days after administration of levosimendan. An internal control group was created by propensity score matching.ResultsA total of 794 patients receiving levosimendan were screened and 368 unique patients were included. Patients were predominantly male (73.6%) and median age was 63 years. Patients were divided by eGFR into three groups: &gt;60 ml/min/1.73 m2 (n = 110), 60–30 ml/min/1.73 m2 (n = 130), and &lt;30 ml/min/1.73 m2 (n = 128). ICU survival was significantly lower in patients with lower eGFR (69.1, 57.7, and 50.8%, respectively, p = 0.016) and patients with lower eGFR were significantly older and had significantly more comorbidities. The primary combined endpoint was reached in 61.8, 63.1, and 69.5% of subjects, respectively (p = 0.396). A multivariate logistic regression model suggested only age (p &lt; 0.020), extracorporeal membrane oxygenation (p &lt; 0.001) or renal replacement therapy (p = 0.028) during day 1–7 independently predict the primary endpoint while kidney function did not (p = 0.835). A propensity score matching of patients with eGFR &lt; 30 and &gt;30 ml/min/1.73 m2 based on these predictors of outcome confirmed the primary endpoint (p = 0.886).ConclusionThe combined endpoint of supraventricular-, ventricular tachycardia and death within 7 days was reached at a similar rate in patients independently of kidney function. Prospective randomized trials are warranted to clarify if levosimendan can be used safely in severely reduced kidney function.
HRas and Myc synergistically induce cell cycle progression and apoptosis of murine cardiomyocytes
Authors: Boikova Aleksandra , Bywater Megan J. , Quaife-Ryan Gregory A. , Straube Jasmin , Thompson Lucy , Ascanelli Camilla , Littlewood Trevor D. , Evan Gerard I. , Hudson James E. , Wilson Catherine H. 
Published: October 2022

AimAdult mammalian cardiomyocytes are incapable of significant proliferation, limiting regeneration after myocardial injury. Overexpression of the transcription factor Myc has been shown to drive proliferation in the adult mouse heart, but only when combined with Cyclin T1. As constitutive HRas activity has been shown to stabilise Cyclin T1 in vivo, we aimed to establish whether Myc and HRas could also act cooperatively to induce proliferation in adult mammalian cardiomyocytes in vivo.Methods and resultsUsing a genetically modified mouse model, we confirmed that constitutive HRas activity (HRasG12V) increased Cyclin T1 expression. HRasG12V and constitutive Myc expression together co-operate to drive cell-cycle progression of adult mammalian cardiomyocytes. However, stimulation of endogenous cardiac proliferation by the ectopic expression of HRasG12V and Myc also induced cardiomyocyte death, while Myc and Cyclin T1 expression did not.ConclusionCo-expression of Cyclin T1 and Myc may be a therapeutically tractable approach for cardiomyocyte neo-genesis post injury, while cell death induced by HRasG12V and Myc expression likely limits this option as a regenerative therapeutic target.
Hemodynamic evaluation of endarterectomy and stenting treatments for carotid web
Authors: Ren Shuqi , Liu Qijia , Chen Zengsheng , Deng Xiaoyan , Sun Anqiang , Luan Jingyuan 
Published: October 2022

Background and purposeA carotid web is a thin, shelf-like luminal protrusion in the internal carotid artery that might cause carotid stenosis and stroke by inducing disturbed flow patterns, thrombosis, and abnormal biomechanical stimulus to the endothelial cells. This study simulated and evaluated how the two main treatments (endarterectomy and stenting) influence hemodynamic environments in the carotid artery and distal carotid siphon arteries, aiming to provide more references for the selection of clinical treatment.Materials and methodsThe carotid web, endarterectomy, and stenting models were reconstructed based on CT images. The blood flow simulations were conducted, and critical parameters related to thrombosis formation and artery remodeling, including swirling strength, wall shear stress (WSS), vortex Q-criterion, and oscillating shear index (OSI), were analyzed.ResultsIn the model of the carotid web, obvious recirculation formed distal to the web, accompanied by lower velocity, lower WSS, higher relative resident time (RRT), and higher Q value. While in both two treatment models, the velocity increased and the Q value and RRT decreased at the carotid bifurcation. In addition, both treatments provide more kinetic energy to the distal carotid siphon artery, especially the stenting model.ConclusionThe carotid web can significantly influence the flow environments in the carotid artery. Both endarterectomy and stenting treatments could significantly diminish the side effects of the web and are feasible choices for web patients in terms of hemodynamics. Besides, the treatments for the carotid web would also influence the flow patterns at the distal carotid siphon, especially for the stenting treatment. But more innovational designs are needed to make the minimally invasive stenting treatment more beneficial.