bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Do I have the capacity to make capacity judgements?’ Researcher reflections from a person-centred dementia support study
Authors: Griffiths Sarah , Gude Alex , Greene Leanne , Weston Lauren , Sutcliffe Caroline L , Wheat Hannah , Oh Tomasina M , Byng Richard 
Published: February 2022

Background and purpose Adults lacking capacity are under-represented in research; therefore, the evidence-base surrounding their support needs is inferior compared to other populations. Involving this group in research is fraught with challenges, including researcher uncertainties about how to carry out capacity judgements. Whilst ethical guidelines and principles provide overarching guidance, there is a lack of detailed guidance and evidence-based training, incorporating practical ‘on the ground’ strategies and advice on communication practices. Experiences and reflections on research procedures used to gauge and address capacity are under reported, resulting in a lack of shared knowledge within the field. Design To help address this, we engaged in researcher (co)meta-reflection on the informed capacity judgement procedure for initial consent, within our current, person-centred dementia intervention feasibility study. Our objective was to identify areas to improve our approach, but to also put forward suggestions for wider change within ethical research practice. Results Findings reveal challenges and facilitators relating to six areas: ‘Conducting time sensitive research whilst remaining person-centred and building relationships’; ‘Information sharing and supporting communication’; ‘Applying the process flexibly’; ‘The role of the carer and the consultee process’; ‘Judging assent and dissent’ and ‘Researcher related factors’. We questioned our ‘capacity to make capacity judgements’ in terms of both our skills and research time constraints. Conclusions Based on our experiences, we argue for greater open discussion between researchers, Patient and Public Involvement contributors and Research Ethics Committees at initial project planning stages. We recommend training and guidance focuses on building researcher skills in applying a standard process flexibly, emphasising naturalistic, conversational approaches to capacity judgement. A crucial consideration for funders is how this time-intensive and sensitive work should be factored into bid application templates and funding grants. Learnings from this article have potential to inform evidence-based guidance and training for researchers, consultees, funders, reviewers and ethics committees.
Clinical Results of Arthroscopic Partial Trapeziectomy With Suture-Button Suspensionplasty for Thumb Carpometacarpal Arthritis
Authors: Maeda Akemi , Ikeguchi Ryosuke , Noguchi Takashi , Yamawaki Rie , Nankaku Manabu , Ando Maki , Yoshimoto Koichi , Sakamoto Daichi , Matsuda Shuichi 
Published: February 2022

Background: This study represents the clinical results, especially range of motion (ROM) improvement, of arthroscopic partial trapeziectomy with suture-button suspensionplasty for symptomatic grade II and III thumb carpometacarpal arthritis with a minimum 1-year follow-up. Methods: Thirty-two patients (mean: 67.5 years) with grade II and III thumb carpometacarpal arthritis treated with arthroscopic partial trapeziectomy with suture-button suspensionplasty were retrospectively followed up for at least 1 year. The physical assessments included ROM, pain visual analogue scale (VAS), strength, and the Disabilities of the Arm, Shoulder, and Hand (DASH) questionnaire. The physical variables were retrospectively compared before surgery and at the final follow-up. Results: Preoperative radial abduction and palmar abduction (45.4 ± 16.4° and 54.3 ± 13.9°, respectively) were significantly increased at the final follow-up (59.7 ± 16.9° and 65.5 ± 14.2°, respectively). Preoperative VAS score, pinch strength, and DASH score (70.5 ± 14.0, 57.2 ± 24.8% and 36.8 ± 14.8, respectively) were also significantly improved at the final follow-up (7.9 ± 9.1, 91.0 ± 39.6%, and 11.7 ± 10.5, respectively). Complications involved 1 case of irritation of the superficial branch of the radial nerve and 1 case of dystonia. Two suture-buttons were removed due to patient discomfort. Conclusions: A significant increase in ROM and pain relief was obtained after suture-button suspensionplasty with arthroscopic partial trapeziectomy.
Compliance to hand hygiene and its determinant factors among Community Health Care Providers in Community Clinics: an observational study in Bangladesh
Authors: Rahman Md Hafizur , Hassan Md Nazmul , Khan Md Shafiqul Islam , Hasanuzzaman Md , Awal Nurullah 
Published: February 2022

Background Healthcare-associated infections caused by poor hand hygiene (HH) practices can affect both the care seekers and the health care providers (HCPs) while providing primary health care. No study has been conducted on the compliance of HH practices among the primary level HCPs in Bangladesh. Objective The study aimed to assess the compliance rates of HH and its determinant factors among Community Health Care Providers (CHCPs) in Community Clinics (CC). Methods A cross-sectional study was conducted from September 2019 to February 2020 among 150 randomly selected CHCPs with functional HH facilities in Patuakhali district, Bangladesh. Structured interviews and observation tools were used to collect data. Results Good HH compliance among CHCPs was found to be 16.7% (95% CI: 11.3–21.3). Out of all 1218 possible HH opportunities, only 255 (20.9%) resulted in any HH action. Presence of 70% alcohol-based hand sanitiser at the point of care (aOR: 6.4, 95% CI: 1.1–38.3), HH training (aOR: 4.6, 95% CI: 1.1–18.9), displayed visual cues (aOR: 4.4, 95% CI: 1.1–17.7), knowledge about HH (aOR: 3.8, 95% CI: 1.1–13.6) and number of HH opportunities (aOR: 0.6, 95% CI: 0.4–0.8) were factors associated with HH compliance. Discussion Overall HH compliance among CHCPs was relatively low. It was recommended to implement multimodal HH improvement strategies, including a continuous training program, supply of alcohol-based hand rub, reminder, provision of five moments of HH in the training modules and feedback on HH performance.
Implementing a Neurohospitalist Program Improves Stroke Care Metrics and Patient Satisfaction Scores
Authors: Pierre-Louis Renée E. , Pannikodu Kelly , Madhoun Maher , Hartnett Josette , Rose Suzanne 
Published: February 2022

Objective Compare the differences in health outcomes and patient satisfaction between a neurohospitalist model of care and a community-based neurologists model at a single community-based teaching hospital among in-patients diagnosed with a cerebrovascular accident (CVA). Methods Data was collected from the Stamford Hospital’s electronic medical records system. An assessment of patient health outcomes and satisfaction scores was conducted, comparing both discrete and continuous variables between the two time periods. An omnibus P-value of 0.05 ( P < 0.05) was considered statistically significant. Results The sample consisted of 341 patients between the two periods, pre-period n = 168 (49.3%) post-period n = 173 (50.7%). Door to lab and door to tPA times decreased significantly between pre- and post-periods ( P = 0.003 and P = 0.002, respectively) as did the number of MRIs ( P < 0.001). In addition, statistically significant increases were found between pre-period and post-period percentages, all increasing over time: stroke education ( P < 0.001), discharged on anticoagulant medication ( P < 0.001), and discharged on anti-thrombolytic medication ( P = 0.019). Patient satisfaction scores demonstrated mean gain across both periods for five of six items. Two items “Doctor’s Concern of my Questions/Worries” and “Skill of Doctors” demonstrated statistical significance ( P = 0.020 and P = 0.029, respectively). Conclusions The introduction of a neurohospitalist service at a community-based teaching hospital improved patient health outcomes on time to intervention, stroke education, discharge medications as well as patient satisfaction. Therefore, it may be beneficial for hospitals to implement a neurohospitalist model of care for their patients presenting with CVA.
Mössbauer and Raman characterization of iron-loaded woody charcoal: effects of Fe3+-dispersion in wood on reduction of Fe3+ and graphitization in carbonization
Authors: Yamagishi Takayuki , Yamauchi Shigeru , Shibutani Sakae , Suzuki Hikaru , Takayama Tsutomu , Sakai Yoichi 
Published: February 2022

AbstractAn Fe3+-impregnation method was improved to disperse Fe3+ in wood powder more homogeneously, and the wood powder was carbonized at various temperatures. The obtained iron-loaded charcoal samples were analyzed using Mössbauer and Raman spectroscopy to understand the effects of the improved method on the reduction of Fe3+ and graphitization. G′(2D)-band was adopted as a marker of graphitic structure formation among Raman scattering due to sp2-carbon. Mössbauer and Raman characterization confirmed that the reduction from Fe3+ to Fe0 is closely related with the graphitization in charcoal; however, both the chemical changes progressed rapidly from a much lower temperature-range and graphitization occurred more uniformly compared with charcoal synthesized using the previous method containing no decompression-impregnation processes. Moreover, the effects of holding time at carbonization temperature and Fe3+ content in the wood powder were examined in terms of relationship between the reduction of Fe3+ and graphitization. It was suggested that there were multiple reaction paths to graphitization in carbonization.
The relationship of weather with daily physical activity and the time spent out of home in older adults from Germany – the ActiFE study
Authors: Klimek Matthias , Peter Raphael Simon , Denkinger Michael , Dallmeier Dhayana , Rapp Kilian , Rothenbacher Dietrich , Klenk Jochen ,   the ActiFE study group, Böhm B. , Geiger H. , Lukas A. , Stingl J. , Riepe M. , Rapp K. , Scharffetter-Kochanek K. , Koenig W. , Steinacker J. M. , Ludolph A. , Nagel G. , Peter R. 
Published: February 2022

AbstractBackgroundThere is a need for a comprehensive evaluation of the associations between varieties of weather conditions on the time spent out-of-home (TOH) and on walking duration (WD) among older adults. We aim to investigate the extent to which various weather parameters (temperature, solar radiation, sunshine duration, humidity, windspeed, and rain) determine daily WD the TOH in older adults.MethodsThe ActiFE (Activity and Function in Older People in Ulm) study is a prospective study of participants aged 65 years or older who wore an accelerometer and kept a movement diary in up to three temporally separated waves from 2009 to 2018 for a duration up to seven days per wave (up to three weeks in summary). We used weather data from a weather station near the participants‘ homes. Age-adjusted and gender-stratified generalized mixed models were used to predict WD and TOH (with 95% confidence interval (CI)) within and between weather categories. Generalized additive models were computed for the single predictions at the weather quartile boundaries. Cubic splines (with 95% pointwise confidence bands (CB)) visualized the continuous course of the weather values.ResultsHigher temperatures, solar radiation and more hours of sunshine, led to an increase in WD and TOH, while higher precipitation, humidities and windspeeds led to a decrease. Women had in general higher WD and TOH times than men.ConclusionsOur data suggest that weather parameters have a considerable influence on PA and TOH. Future analyses and interpretation of PA data should therefore account for weather parameters.
Genomic architecture of phenotypic extremes in a wild cervid
Authors: Anderson S. J. , Côté S. D. , Richard J. H. , Shafer A. B. A. 
Published: February 2022

AbstractIdentifying the genes underlying fitness-related traits such as body size and male ornamentation can provide tools for conservation and management and are often subject to various selective pressures. Here we performed high-depth whole genome re-sequencing of pools of individuals representing the phenotypic extremes for antler and body size in white-tailed deer (Odocoileus virginianus). Samples were selected from a tissue repository containing phenotypic data for 4,466 male white-tailed deer from Anticosti Island, Quebec, with four pools representing the extreme phenotypes for antler and body size after controlling for age. Our results revealed a largely homogenous population but detected highly divergent windows between pools for both traits, with the mean allele frequency difference of 14% for and 13% for antler and body SNPs in outlier windows, respectively. Genes in outlier antler windows were enriched for pathways associated with cell death and protein metabolism and some of the most differentiated windows included genes associated with oncogenic pathways and reproduction, processes consistent with antler evolution and growth. Genes associated with body size were more nuanced, suggestive of a highly complex trait. Overall, this study revealed the complex genomic make-up of both antler morphology and body size in free-ranging white-tailed deer and identified target loci for additional analyses.
Natterin-like depletion by CRISPR/Cas9 impairs zebrafish (Danio rerio) embryonic development
Authors: Seni-Silva Ana Carolina , Maleski Adolfo Luis Almeida , Souza Milena Marcolino , Falcao Maria Alice Pimentel , Disner Geonildo Rodrigo , Lopes-Ferreira Monica , Lima Carla 
Published: February 2022

Abstract
Background
The Natterin protein family was first discovered in the venom of the medically significant fish Thalassophryne nattereri, and over the last decade natterin-like genes have been identified in various organisms, notably performing immune-related functions. Previous findings support natterin-like genes as effector defense molecules able to activate multiprotein complexes driving the host innate immune response, notably due to the pore-forming function of the aerolysin superfamily members. Herein, employing a combination of the CRISPR/Cas9 depletion system, phenotype-based screening, and morphometric methods, we evaluated the role of one family member, LOC795232, in the embryonic development of zebrafish since it might be implicated in multiple roles and characterization of the null mutant is central for analysis of gene activity.

Results
Multiple sequence alignment revealed that the candidate natterin-like has the highest similarity to zebrafish aep1, a putative and better characterized fish-specific defense molecule from the same family. Compared to other species, zebrafish have many natterin-like copies. Whole-mount in situ hybridization confirmed the knockout and mutant embryos exhibited epiboly delay, growth retardation, yolk sac and heart edema, absent or diminished swim bladder, spinal defects, small eyes and head, heart dysfunction, and behavioral impairment. As previously demonstrated, ribonucleoproteins composed of Cas9 and duplex guide RNAs are effective at inducing mutations in the F0 zebrafish.

Conclusions
The considerably high natterin-like copies in zebrafish compared to other species might be due to the teleost-specific whole genome duplication and followed by subfunctionalization or neofunctionalization. In the present work, we described some of the natterin-like features in the zebrafish development and infer that natterin-like proteins potentially contribute to the embryonary development and immune response.
Association between circadian variation of heart rate and mortality among critically ill patients: a retrospective cohort study
Authors: Zhang Jingjing , Du Linyun , Li Jiamei , Li Ruohan , Jin Xuting , Ren Jiajia , Gao Ya , Wang Xiaochuang 
Published: February 2022

Abstract
Background
Heart rate (HR) related parameters, such as HR variability, HR turbulence, resting HR, and nighttime mean HR have been recognized as independent predictors of mortality. However, the influence of circadian changes in HR on mortality remains unclear in intensive care units (ICU). The study is designed to evaluate the relationship between the circadian variation in HR and mortality risk among critically ill patients.

Methods
The present study included 4,760 patients extracted from the Multiparameter Intelligent Monitoring in Intensive Care II database. The nighttime mean HR/daytime mean HR ratio was adopted as the circadian variation in HR. According to the median value of the circadian variation in HR, participants were divided into two groups: group A (≤ 1) and group B (> 1). The outcomes included ICU, hospital, 30-day, and 1-year mortalities. The prognostic value of HR circadian variation was investigated by multivariable logistic regression models and Cox proportional hazards models.

Results
Patients in group B (n = 2,471) had higher mortality than those in group A (n = 2,289). Multivariable models revealed that the higher circadian variation in HR was associated with ICU mortality (odds ratio [OR], 1.393; 95% confidence interval [CI], 1.112–1.745; P = 0.004), hospital mortality (OR, 1.393; 95% CI, 1.112–1.745; P = 0.004), 30-day mortality (hazard ratio, 1.260; 95% CI, 1.064–1.491; P = 0.007), and 1-year mortality (hazard ratio, 1.207; 95% CI, 1.057–1.378; P = 0.005), especially in patients with higher SOFA scores.

Conclusions
The circadian variation in HR might aid in the early identification of critically ill patients at high risk of associated with ICU, hospital, 30-day, and 1-year mortalities.
Construction of genetic classification model for coronary atherosclerosis heart disease using three machine learning methods
Authors: Peng Wenjuan , Sun Yuan , Zhang Ling 
Published: February 2022

Abstract
Background
Although the diagnostic method for coronary atherosclerosis heart disease (CAD) is constantly innovated, CAD in the early stage is still missed diagnosis for the absence of any symptoms. The gene expression levels varied during disease development; therefore, a classifier based on gene expression might contribute to CAD diagnosis. This study aimed to construct genetic classification models for CAD using gene expression data, which may provide new insight into the understanding of its pathogenesis.

Methods
All statistical analysis was completed by R 3.4.4 software. Three raw gene expression datasets (GSE12288, GSE7638 and GSE66360) related to CAD were downloaded from the Gene Expression Omnibus database and included for analysis. Limma package was performed to identify differentially expressed genes (DEGs) between CAD samples and healthy controls. The WGCNA package was conducted to recognize CAD-related gene modules and hub genes, followed by recursive feature elimination analysis to select the optimal features genes (OFGs). The genetic classification models were established using support vector machine (SVM), random forest (RF) and logistic regression (LR), respectively. Further validation and receiver operating characteristic (ROC) curve analysis were conducted to evaluate the classification performance.

Results
In total, 374 DEGs, eight gene modules, 33 hub genes and 12 OFGs (HTR4, KISS1, CA12, CAMK2B, KLK2, DDC, CNGB1, DERL1, BCL6, LILRA2, HCK, MTF2) were identified. ROC curve analysis showed that the accuracy of SVM, RF and LR were 75.58%, 63.57% and 63.95% in validation; with area under the curve of 0.813 (95% confidence interval, 95% CI 0.761–0.866, P < 0.0001), 0.727 (95% CI 0.665–0.788, P < 0.0001) and 0.783 (95% CI 0.725–0.841, P < 0.0001), respectively.

Conclusions
In conclusion, this study found 12 gene signatures involved in the pathogenic mechanism of CAD. Among the CAD classifiers constructed by three machine learning methods, the SVM model has the best performance.
Diagnosis and treatment of traumatic duodenal rupture in children
Authors: Luo Yuanyuan , He Xiaobing , Geng Lanlan , Ouyang Runxian , Xu Yingyi , Liang Yufeng , Wu Jinhui , Zhang Hong , Ye Zhihua , Zou Rongjun , Wu Qiang , Chai Chengwei 
Published: February 2022

AbstractBackgroundThe purpose of this study was to investigate the diagnosis and treatment experience of traumatic duodenal ruptures in children.MethodsClinical data were collected from four children suffering from a traumatic duodenal rupture who were admitted to and treated by our hospital from January 2012 to December 2020. The early diagnosis and treatment, surgical plan, postoperative management, complications, and prognosis of each child were analyzed. The key points and difficulties of the diagnosis and treatment for this type of injury are summarized.ResultsOne child had an extreme infection caused by drug-resistant bacteria, which resulted in severe complications, including wound infection, dehiscence, and an intestinal fistula. One child developed an anastomotic stenosis after the duodenostomy, which improved following an endoscopic balloon dilatation. The other two children had no relevant complications after their operations. All four patients were cured and discharged from hospital. The average hospital stay was 48.25 ± 26.89 days. The follow-up period was 0.5 to 1 year. No other complications occurred, and all children had a positive prognosis.ConclusionsThe early identification of a duodenal rupture is essential, and surgical exploration should be carried out proactively. The principles of damage-control surgery should be followed as much as possible during the operation. Multidisciplinary cooperation and management are both important to reduce the occurrence of postoperative complications and improve cure rates.
Dizziness and benign paroxysmal positional vertigo among retirement home residents: a cross-sectional descriptive and interventional study
Authors: Müller Rainer , Zangger Peter , Straumann Dominik , Bögli Stefan Y. 
Published: February 2022

Abstract
Background
The prevalence of dizziness increases with age. We aimed to determine the point prevalence of dizziness and, in particular, of benign paroxysmal positional vertigo (BPPV) among retirement home residents. Furthermore, we aimed to evaluate the efficacy of a 2-axis turntable based BPPV treatment.

Methods
We contacted all large retirement homes in or around the city of Zurich (Switzerland). 10 retirement homes (with a total of 536 residents) agreed to participate in this study. 83 rejected inquiries by residents led to a potential study population of 453 residents. After a structured interview evaluating the presence and characteristics of dizziness, all willing patients were tested for positional vertigo and nystagmus on a portable and manually operated 2-axis turntable that was transported to the retirement home. Testing consisted of the Dix-Hallpike and supine roll maneuvers to both sides. Participants were immediately treated with the appropriate liberation maneuver whenever BPPV was diagnosed. Otherwise, taking the resident’s medical history, a neuro-otological bedside examination, and a review of the available medical documentation was used to identify other causes of dizziness.

Results
Out of the 453 residents, 75 (16.6%; average age: 87.0 years; 68% female) were suffering from dizziness presently or in the recent past and gave their consent to participate in this study. Among the participants tested on the turntable (n = 71), BPPV was present in 11.3% (point prevalence). Time-related properties, triggering factors and qualitative attributes of vertigo or dizziness were not significantly different between the dizzy participants with and those without BPPV. In all BPPV patients, appropriate liberation maneuvers were successful.

Conclusions
BPPV could be demonstrated in about one tenth of retirement home residents with dizziness or recent dizziness. Such point prevalence of BPPV translates to a much higher yearly prevalence if one assumes that BPPV is not present on every day. Our finding suggests that retirement home residents suffering from dizziness should be regularly tested for BPPV and treated with appropriate liberation maneuvers, ideally on turntable to reduce strain.

Trial registration
ClinicalTrials.gov Identifier NCT03643354.