bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proliferative Exhausted CD8 T Cells Exacerbate Long-lasting Anti-tumor Effects in Human Papillomavirus Positive Head and Neck Squamous Cell Carcinoma
Authors: Cheng Danni , Qiu Ke , Rao Yufang , Mao Minzi , Li Li , Wang Yan , Song Yao , Chen Junren , Yi Xiaowei , Shao Xiuli , Huang Shao Hui , Zhang Yi , Chen Xuemei , Wu Sisi , Yu Shuaishuai , Liu Jun , Wang Haiyang , Peng Xingchen , Li Daibo , Yang Lin , Chen Li , Ying Zhiye , Zheng Yongbo , Zheng Meijun , Ying Binwu , Zeng Xiaoxi , Zhang Wei , Xu Wei , Liu Geoffrey , Chen Fei , Yu Haopeng , Zhao Yu , Ren Jianjun 
Published: October 2022

AbstractThe survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated CD8+exhausted T cells (Tex) in HNSCC. We performed cell level multi-omics sequencing on human HNSCC samples to decipher the multi-dimensional characteristics of Tex cells. A proliferative exhausted CD8+T cell cluster (P-Tex) which was beneficial to survival outcomes of patients with HPV-positive HNSCC was identified. Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. P-Tex cells could aggregate in the antigen presenting cell niches and activate certain signaling pathways. Together, our findings suggest a promising role for P-Tex cells in the prognosis of patients with HPV-positive HNSCC by providing modest but persistent anti-tumor effects.Graphic abstract
Experimental Diets Dictate the Metabolic Benefits of Probiotics in Obesity
Authors: Søgaard Larsen Ida , Choi Béatrice S.-Y. , Föh Bandik , Kristensen Nanna Ny , Ouellette Adia , Haller Rune Falkenberg , Olsen Peter Bjarke , Saulnier Delphine , Sina Christian , Jensen Benjamin A. H. , Marette André 
Published: October 2022

AbstractBackgroundGrowing evidence supports the use of probiotics to prevent or mitigate obesity-related dysmetabolism and non-alcoholic fatty liver disease (NAFLD). However, frequent reports of responders versus non-responders to probiotic treatment warrant a better understanding of key modifiers of host-microbe interactions. The influence of host diet on probiotic efficacy, in particular against metabolic diseases, remains elusive.MethodWe fed C57BL6/J mice a low fat reference diet or one of two energy-matched high fat and high sucrose diets for 12 weeks; a classical high fat diet (HFD) and a customized fast food-mimicking diet (FFMD). During the studies, mice fed either obesogenic diet were gavaged daily with one of two probiotic lactic acid bacteria (LAB) strains previously classified asLactobaccillus, namelyLimosilactobacillus reuteri(L. reuteri) orLacticaseibacillus paracaseisubsp.paracasei(L. paracasei), or vehicle.ResultsThe tested probiotics exhibited a reproducible efficacy but dichotomous response according to the obesogenic diets used. Indeed,L. paracaseiprevented weight gain, improved insulin sensitivity, and protected against NAFLD development in mice fed HFD, but not FFMD. Conversely,L. reuteriimproved glucoregulatory capacity, reduced NAFLD development, and increased distal gut bile acid levels associated with changes in predicted functions of the gut microbiota exclusively in the context of FFMD-feeding.ConclusionWe found that the probiotic efficacy of two LAB strains is highly dependent on experimental obesogenic diets. These findings highlight the need to carefully consider the confounding impact of diet in order to improve both the reproducibility of preclinical probiotic studies and their clinical research translatability.Graphical abstract
Green synthesis of ZnO nanoparticles usingCocos nuciferaleaf extract: Characterization, antimicrobial, antioxidant, and photocatalytic activity
Authors: Rahman Farjana , Patwary Md Abdul Majed , Siddique Md. Abu Bakar , Bashar Muhammad Shahriar , Haque Md. Aminul , Akter Beauty , Rashid Rimi , Haque Md. Anamul , Uddin A. K. M. Royhan 
Published: October 2022

AbstractZinc oxide nanoparticles (ZnO NPs) have been successfully prepared usingCocos nuciferaleaf extract and investigated with their antimicrobial, antioxidant, and photocatalytic activity. The structural, compositional, and morphological properties of the NPs were recorded and studied systematically to confirm the synthesis. The aqueous suspension of NPs showed a UV-Vis. absorption maxima of 370 nm indicating primarily its formation. The XRD analysis identified the NPs with hexagonal wurtzite structure with an average particle size of 16.6 nm. The FTIR analysis identified some biomolecules and functional groups in the leaf extract as responsible for the encapsulation and stabilization of ZnO NPs. The EDX analysis showed the desired elemental compositions in the material. A flower-shaped morphology of ZnO NPs was observed by SEM with a grain size of around 15 nm. The optical properties of the NPs were studied by UV-Vis spectroscopy and the band gap was calculated as 3.37 eV. The prepared ZnO NPs have demonstrated antimicrobial activity againstT. harzianum and S. aureuswith a ZOI (zone of inhibition) of 14 and 10 mm, respectively. The photocatalytic behavior of ZnO NPs showed absorbance degradation at around 640 nm and discolored methylene blue dye after one hour with a degradation maximum of 84.29 %. Thus, the prepared ZnO NPs could be used potentially in antibiotic development, pharmaceutical industries, and as photocatalysts.
Molecular basis of the pH-controlled maturation of the tick-borne encephalitis flavivirus
Authors: Bignon Emmanuelle , Dumont Elise , Monari Antonio 
Published: October 2022

AbstractFlaviviruses are enveloped viruses causing high public concerns. Their maturation spans several cellular compartments having different pH. Thus, complex control mechanisms are in place to avoid premature maturation. Here we report the dynamical behavior at neutral and acidic pH of the precursor of the membrane fusion protein E of tick-borne encephalitis, showing the different stabilization of the E dimer and the role played by the small fusion-assisting protomer (pr). The comprehension, at atomic resolution, of the fine regulation of viral maturation will be fundamental to the development of efficient strategies against emerging viral threats.
HNRNPH1 regulates the neuroprotective cold-shock protein RBM3 expression through poison exon exclusion
Authors: Lin Julie Qiaojin , Khuperkar Deepak , Pavlou Sofia , Makarchuk Stanislaw , Patikas Nikolaos , Lee Flora C.Y. , Kang Jianning , Field Sarah F. , Zbiegly Julia M. , Freeman Joshua L. , Ule Jernej , Metzakopian Emmanouil , Ruepp Marc-David , Mallucci Giovanna R. 
Published: October 2022

SummaryEnhanced expression of the cold-shock protein RNA binding motif 3 (RBM3) is highly neuroprotective bothin vitroandin vivo. Whilst upstream signalling pathways leading to RBM3 expression have been described, the precise molecular mechanism of RBM3 induction during cooling remains elusive. To identify temperature-dependent modulators of RBM3, we performed a genome-wide CRISPR-Cas9 knockout screen using RBM3-reporter human iPSC-derived neurons. We found that RBM3 mRNA and protein levels are robustly regulated by several splicing factors, with heterogeneous nuclear ribonucleoprotein H1 (HNRNPH1) being the strongest positive regulator. Splicing analysis revealed that moderate hypothermia significantly represses the inclusion of a poison exon, which, when retained, targets the mRNA for nonsense-mediated decay. Importantly, we show that HNRNPH1 mediates this cold-dependent exon skipping via its interaction with a G-rich motif within the poison exon. Our study provides novel mechanistic insights into the regulation of RBM3 and provides further targets for neuroprotective therapeutic strategies.Graphical Abstract
Elevated estradiol during a hormone simulated pseudopregnancy decreases sleep and increases hypothalamic activation in female Syrian hamsters
Authors: Irvine Abiola , Gaffney Maeve I. , Haughee Erin K. , Horton Marité A. , Morris Hailey C. , Harris Kagan C. , Corbin Jaclyn E. , Merrill Clara , Perlis Michael L. , Been Laura E. 
Published: October 2022

AbstractSleep disruptions are a common occurrence during the peripartum period. While physical and environmental factors associated with pregnancy and newborn care account for some sleep disruptions, there is evidence that peripartum fluctuations in estrogens may independently impact sleep. We therefore used a hormone-simulated pseudopregnancy in female Syrian hamsters to test the hypothesis that pregnancy-like increases in estradiol decrease sleep in the absence of other factors. Adult female Syrian hamsters were ovariectomized and given daily hormone injections that simulate estradiol levels during early pregnancy, late pregnancy, and the postpartum period. Home cage video recordings were captured at seven timepoints and videos were analyzed for actigraphy. During “late pregnancy,” total sleep time and sleep efficiency were decreased in hormone-treated animals during the white light period compared to vehicle controls. During both “early pregnancy” and “late pregnancy,” locomotion was increased in the white light period for hormone-treated animals; this change continued into the “postpartum period” for animals who continued to receive estradiol treatment, but not for animals who were withdrawn from estradiol. At the conclusion of the experiment, animals were euthanized and cFos expression was quantified in the ventral lateral preoptic area (VLPO) and lateral hypothalamus (LH). Animals who continued to receive high levels of estradiol during the “postpartum” period had significantly more cFos in the VLPO and LH than animals who were withdrawn from hormones or vehicle controls. Together, these data suggest that increased levels of estradiol during pregnancy are associated with sleep suppression which may be mediated by increased activation of hypothalamic nuclei.
Thiovibrio frasassiensisgen. nov., sp. nov., an autotrophic, elemental sulfur disproportionating bacterium isolated from sulfidic karst sediment, and proposal of Thiovibrionaceae fam. nov.
Authors: Aronson H.S. , Thomas C. , Bhattacharyya M.K. , Eckstein S.R. , Jensen S.R. , Barco R.A. , Macalady J.L. , Amend J.P. 
Published: October 2022

AbstractA novel, autotrophic, mesophilic bacterium, strain RS19-109T, was isolated from sulfidic stream sediments in the Frasassi Caves, Italy. The cells of this strain grew chemolithoautotrophically under anaerobic conditions while disproportionating elemental sulfur (S0) and thiosulfate, but not sulfite with bicarbonate/CO2as a carbon source. Autotrophic growth was also observed with molecular hydrogen as an electron donor, and S0, sulfate, thiosulfate, nitrate, and ferric iron as electron acceptors. Oxygen was not used as an electron acceptor and sulfide was not used as an electron donor. Weak growth was observed with sulfate as an electron acceptor and organic carbon as electron donors and carbon sources. The strain also showed weak growth by fermentation of tryptone. Strain RS19-109Twas found to be phylogenetically distinct based on 16S rRNA gene sequence similarity (89.2%) to its closest relative,Desulfurivibrio alkaliphilusAHT2T. The draft genome sequence for strain RS19-109Thad average nucleotide identity, average amino acid identity, andin silicoDNA-DNA hybridization values of 72.2%, 63.0%, and 18.3%, respectively, compared with the genome sequence ofD. alkaliphilusAHT2T. On the basis of its physiological and genomic properties, strain RS19-109Tis proposed as the type strain of a novel species of a novel genus,Thiovibrio frasassiensisgen. nov., sp. nov. A novel family,Thiovibrionaceaefam. nov., is proposed to accommodateThiovibriowithin the order Desulfobulbales.
A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients
Authors: Pita-Juarez Yered , Karagkouni Dimitra , Kalavros Nikolaos , Melms Johannes C. , Niezen Sebastian , Delorey Toni M. , Essene Adam L , Brook Olga R. , Pant Deepti , Skelton-Badlani Disha , Naderi Pourya , Huang Pinzhu , Pan Liuliu , Hether Tyler , Andrews Tallulah S. , Ziegler Carly G.K. , Reeves Jason , Myloserdnyy Andriy , Chen Rachel , Nam Andy , Phelan Stefan , Liang Yan , Amin Amit Dipak , Biermann Jana , Hibshoosh Hanina , Veregge Molly , Kramer Zachary , Jacobs Christopher , Yalcin Yusuf , Phillips Devan , Slyper Michal , Subramanian Ayshwarya , Ashenberg Orr , Bloom-Ackermann Zohar , Tran Victoria M. , Gomez James , Sturm Alexander , Zhang Shuting , Fleming Stephen J. , Warren Sarah , Beechem Joseph , Hung Deborah , Babadi Mehrtash , Padera Robert F. , MacParland Sonya A. , Bader Gary D. , Imad Nasser , Solomon Isaac H. , Miller Eric , Riedel Stefan , Porter Caroline B.M. , Villani Alexandra-Chloé , Tsai Linus T.-Y. , Hide Winston , Szabo Gyongyi , Hecht Jonathan , Rozenblatt-Rosen Orit , Shalek Alex K. , Izar Benjamin , Regev Aviv , Popov Yury , Jiang Z. Gordon , Vlachos Ioannis S. 
Published: October 2022

AbstractThe molecular underpinnings of organ dysfunction in acute COVID-19 and its potential long-term sequelae are under intense investigation. To shed light on these in the context of liver function, we performed single-nucleus RNA-seq and spatial transcriptomic profiling of livers from 17 COVID-19 decedents. We identified hepatocytes positive for SARS-CoV-2 RNA with an expression phenotype resembling infected lung epithelial cells. Integrated analysis and comparisons with healthy controls revealed extensive changes in the cellular composition and expression states in COVID-19 liver, reflecting hepatocellular injury, ductular reaction, pathologic vascular expansion, and fibrogenesis. We also observed Kupffer cell proliferation and erythrocyte progenitors for the first time in a human liver single-cell atlas, resembling similar responses in liver injury in mice and in sepsis, respectively. Despite the absence of a clinical acute liver injury phenotype, endothelial cell composition was dramatically impacted in COVID-19, concomitantly with extensive alterations and profibrogenic activation of reactive cholangiocytes and mesenchymal cells. Our atlas provides novel insights into liver physiology and pathology in COVID-19 and forms a foundational resource for its investigation and understanding.
Women on boards: an empirical study on the effects on financial performance and corporate social responsibility
Authors: Awwad Bahaa Sobhi , Binsaddig Ruaa , Kanan Mohammad , Al Shirawi Thaira 
Published: October 2022

Purpose
The purpose of this study is to investigate whether there is a relationship between women’s presence on boards of directors and companies’ financial performance and corporate social responsibility (CSR) disclosure and, if so, whether this relationship is positive, negative or neutral.

Design/methodology/approach
The research sample included 47 companies listed on the Palestine Stock Exchange from 2010 to 2020. Panel regression analysis was used to examine the study’s hypothesis and achieve the study’s objectives.

Findings
The presence of women on the board of directors positively affects a company’s financial performance and disclosure of CSR. However, measuring the CSR disclosure sub-components separately shows a decrease in the disclosure index towards both the environment and employees. Moreover, the level of female representation on the boards of directors of the Palestinian companies studied is generally low.

Research limitations/implications
Concerning the study limitations, the sample, which comprised all companies listed on the Palestine Stock Exchange from 2010 to 2020, was small. Concerning the implications of the study results, it is recommended that all companies listed on the Palestine Stock Exchange incorporate women on their boards of directors and in their executive management and audit committees.

Practical implications
The presence of women on Palestinian companies’ boards of directors enhances decision-making policy because of the differences between the genders as well as women’s capacity and unique skills.

Originality/value
This research contributes to the literature on women’s representation on the boards of directors of the Palestinian companies listed on the Palestine Stock Exchange with the possibility of issuing mandatory instructions for their existence. This study also attempts to provide a better understanding of the financial performance and disclosure of CSR of companies with women on these boards and helps determine whether the relationships between these variables are positive, negative or neutral. Furthermore, this study attempts to determine the extent of these companies’ commitment to the indicators of CSR disclosure.
Availability of different types of alcohol and amount of intake and next-day hangover – a naturalistic randomized controlled pilot trial
Authors: Grummedal Ole , Zangenberg Marie Strøm , Tolstrup Janne Schurmann 
Published: October 2022

Purpose
The authors tested the hypothesis that having a high availability of different types of alcoholic drinks (beer, wine or spirits) as compared to being confined to one type only associate with a higher alcohol intake and worse next-day hangover.

Design/methodology/approach
This randomized controlled pilot trial took place over one evening and included 35 individuals randomized into two groups. Individuals in the mixed-type group could consume any type of alcoholic drink (beer, wine, cocktails), whereas individuals in the single-type group were to adhere to one type of own choice. Information on number of drinks was obtained continuously during the trial. Hangover symptoms were reported by participants using the Acute Hangover Severity Scale (AHSS) at 8:00, 12:00 and 16:30 the following day. Median regression was used to assess number of drinks and maximum AHSS in two groups.

Findings
Participants in the mixed-type group consumed significantly more drinks compared to the single-type group (10.9 vs 5.7, p < 0.001). The maximal AHSS score the day after drinking was higher in the mixed-type group as compared to the single-type group (median 4.3 vs 2.6, p < 0.0001).

Originality/value
This study suggests that having a variety of alcoholic drinks available associates to a higher alcohol intake and more severe hangover as compared to having to adhere to one type only. Larger studies are warranted to validate findings.
Numerical investigation of the particle circulation characteristics in a tapered fluidized bed
Authors: Che Hanqiao , Chen Tang 
Published: October 2022

PurposeThe objective of this study is to investigate several issues related to particle circulation within the TFB, including exploring an appropriate method to quantify particle circulation time, the effects of different operational parameters on particle circulation time, and the relationship between particle mixing and particle circulation.Design/methodology/approachThe computational fluid dynamics coupled with the discrete element method (CFD-DEM) is applied to investigate the particle circulation characteristics of a tapered fluidized bed (TFB). An approach for defining particle circulation, which accounts for the horizontal motion of each particle, is proposed to estimate particle circulation time.FindingsIt is found that the overall particle circulation in a TFB could be accelerated by increasing air velocity and wall inclination angle, while an increase in particle size and an increase in inter-particle cohesive forces decelerate particle circulation; the increase in the open area ratio of the central region of the air distributor would decelerate the particle circulation. Moreover, the particle circulation time and mixing rate are independent variables that describe the flow dynamics of particles from different perspectives.Practical implicationsA large part of fluidized beds in industrial applications can be classified as TFB. This study presents a numerical method to obtain detailed knowledge about particle circulation in a TFB, which is essential for the design, optimization, and control of related processes.Originality/valueThe particle circulation in a TFB is important but rarely investigated, and it is hard to be quantified using existing experimental approaches. The proposed numerical workflow reveals the characteristics of particle circulation from a particle-scale perspective.
New approach for soliton solutions for the (2 + 1)-dimensional KdV equation describing shallow water wave
Authors: Khuri Suheil 
Published: October 2022

Purpose
The purpose of this study is to produce families of exact soliton solutions (2+1)-dimensional Korteweg-de Vries (KdV) equation, that describes shallow water waves, using an ansätze approach.

Design/methodology/approach
This article aims to introduce a recently developed ansätze for creating soliton and travelling wave solutions to nonlinear nonintegrable partial differential equations, especially those with physical significance.

Findings
A recently developed ansätze solution was used to successfully construct soliton solutions to the (2 + 1)-dimensional KdV equation. This straightforward method is an alternative to the Painleve test analysis, yielding similar results. The strategy demonstrated the existence of a single soliton solution, also known as a localized wave or bright soliton, as well as singular solutions or kink solitons.

Originality/value
The ansätze solution used to construct soliton solutions to the (2 + 1)-dimensional KdV equation is novel. New soliton solutions were also obtained.
Advancing the institutional perspective on informal entrepreneurship: a study of formalization intentions among street entrepreneurs
Authors: Shahid Muhammad Shehryar , Rodgers Peter , Vershinina Natalia , Zehra Mashal E. , Williams Colin C. 
Published: October 2022

PurposeInformal entrepreneurship is seen as a direct outcome of either the failure of formal institutions or the asymmetry between formal and informal institutions. These two viewpoints are so far debated as alternative theoretical explanations for the prevalence of informal entrepreneurship. In this paper, the authors offer a theoretically integrative approach to further advance the institutional perspective of informal entrepreneurship. The purpose of this paper is to address these issues.Design/methodology/approachUsing face-to-face surveys of 322 street entrepreneurs from Lahore, Pakistan, the authors deploy the hitherto unused partial least square approach (PLS) to structural equation modeling (SEM) to analyze data within the field of informal entrepreneurship.FindingsThe empirical findings strongly support the theoretical propositions of the new institutional perspective that the authors present in their paper. The authors find no direct impact of factors like procedural justice, redistributive justice and public sector corruption (i.e. formal institutional failings) on the formalization intentions of street entrepreneurs. Their findings demonstrate that the relationship between formal institutional failings and formalization intentions can only be explained through the mediating role of institutional asymmetry (i.e. tax morality).Research limitations/implicationsFrom a policy perspective, the authors find that if they can encourage street entrepreneurs to obtain a local-level registration as the first step toward formalization, it will significantly increase their chances to opt for higher national-level registrations.Originality/valueThis paper presents a unique attempt to further understand the context of street entrepreneurship through the theoretical lens of the institutional theory. In doing so, it synthesizes the arguments of existing institutional perspectives and further develops the institutional theory of informal entrepreneurship. Moreover, the paper develops the concept of “formalization intentions”.