bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic tissue-specific H2Bub1 is required for human and mouse cardiogenesis
Authors: Barish Syndi , Drozd Jeffrey , Berg Kathryn , Berglund-Brown Isabella , Khizir Labeeqa , Wasson Lauren , Seidman Christine , Seidman Jonathan , Chen Sidi , Brueckner Martina 
Published: April 2022

Abstract
De novo variants affecting the core complex required for monoubiquitination of histone H2B (H2Bub1) are enriched in human congenital heart disease. H2Bub1 is an enigmatic chromatin modification required in stem cell differentiation, cilia function, post-natal cardiomyocyte maturation and transcriptional elongation. However, it is still unknown how H2Bub1 affects cardiogenesis (heart structure formation), which is distinct from cardiomyocyte maturation and underlies congenital heart disease. Here we show that the RNF20-core complex (RNF20-RNF40-UBE2B) is required for cardiogenesis in mouse embryos and is essential for differentiation of human iPSCs into cardiomyocytes. Mice with cardiac-specific deletion of Rnf20 are e12.5 lethal, have thinned myocardium, a deficient ventricular septum, and abnormal cardiac sarcomere organization. We analyzed H2Bub1 marks during the time course of differentiation of human iPSCs into cardiomyocytes, and demonstrated that H2Bub1 marks are erased from a majority of genes at the transition from cardiac mesoderm to cardiac progenitor cells, but are preserved on a subset of long cardiac-specific genes. Sarcomeric gene expression is dependent on normal H2Bub1 both in mice and in human iPSC-derived cardiomyocytes. Finally, we identify an accumulation of H2Bub1 near the center of tissue-specific genes in human cardiomyocytes, mouse embryonic fibroblasts, and human fetal osteoblasts associated with transcriptional elongation efficiency that is absent in UBE2B knock-out H2Bub1-deficient cardiomyocytes. In summary, normal H2Bub1 distribution is required for cardiac morphogenesis and cardiomyocyte differentiation, and we propose that H2Bub1 regulates tissue-specific gene expression by increasing the efficiency of transcriptional elongation.
Body fat percentage, cardiorespiratory fitness and arterial blood pressure in children and adolescents: a longitudinal analysis
Authors: Brand Caroline , Sehn Ana Paula , Fochesatto Camila Felin , Silveira João Francisco de Castro , Mota Jorge , Gomez David Martinez , Gaya Anelise Reis , Reuter Cézane Priscila , Renner Jane Dagmar Pollo 
Published: April 2022

Abstract
Background: a better understanding of how cardiorespiratory fitness (CRF) and adiposity interact to associate with arterial blood pressure over time remains inconclusive. Thus, the aim of the present study was to examine whether changes in cardiorespiratory fitness moderates the association between body fat percentage (BF%) and arterial blood pressure in children and adolescents. Methods: This is an observational longitudinal study with 407 children and adolescents aged 8-17 years followed-up for three years from a city in the Southern Brazil. Participants were evaluated in 2011 and 2014. CRF was measured by validated field-based tests following the Projeto Esporte Brazil protocols and peak oxygen uptake (VO2peak) was estimated. BF% was determined by the measures of tricipital and subscapular skinfolds using equations according to sex. Systolic and diastolic blood pressure (SBP, DBP) were measured with a sphygmomanometer according to standard procedures. Moderation analyses included multiple linear regression models adjusted for sex, age, pubertal status, height, socioeconomic level, skin color, and the arterial blood pressure variable itself at baseline. Results: It was observed a significant inverse association between VO2peak at baseline with SBP (β=-0.646 CI95%=-0.976 -0.316) and DBP (β=-0.649 CI95%=-0.923 -0.375) at follow-up and a positive association between BF% at baseline with SBP (β=0.274; CI95%= 0.094 0.455) and DBP (β=0.301; CI95%=0.150 0.453) at follow-up. In addition, results indicated a significant interaction term between changes in VO2peak and BF% at baseline with both SBP (p=0.034) and DBP at follow-up (p=0.011), indicating that an increase of at least 0.35mL/kg/min and 1.78mL/kg/min in VO2peak attenuated the positive relationship between BF% with SBP and DBP. Conclusion: CRF moderates the relationship between BF% and SBP and DBP in children and adolescents.
Early Versus Delayed Enteral Nutrition in Mechanically Ventilated Patients with Circulatory Shock: A Prospective Multicenter, Multi-National Cohort Study
Authors: Reyes Luis Alfonso Ortiz , Patel Jayshil J. , Jiang Xuran , Yataco Angel Coz , Day Andrew G. , Shah Faraaz , Zelten James , Tamae-Kakazu Maximiliano , Rice Todd , Heyland Daren K. 
Published: April 2022

Abstract
Introduction: Real-world evidence on the timing and efficacy of enteral nutrition (EN) practices in Intensive Care Unit (ICU) patients with circulatory shock are limited. We hypothesized early EN (EEN), as compared to delayed EN (DEN), is associated with improved clinical outcomes in mechanically ventilated (MV) patients with circulatory shock. Methods: We analyzed a dataset from an international, multicenter randomized clinical trial (RCT) evaluating protein dose in ICU patients. EEN was defined as initiating <48 hours from ICU admission and DEN >48 hours. We identified MV patients in circulatory shock to evaluate the association between the timing of EN initiation and clinical outcomes. Results: We included 626 patients. Median age was 60 years [18-93], 55% had septic shock, 99% received norepinephrine alone and 91% received EN alone. Forty-two percent of EEN patients had persistent organ dysfunction syndrome-plus death at day 28, compared to 53% in the DEN group (p=0.04) (adjusted OR 0.60, 95% confidence intervals, 0.38-0.93, p=0.02). EEN was associated with more ICU-free days (9.3±9.2 vs. 5.7±7.9, p=0.0002), more days alive and free of vasopressors (7.1±3.1 vs. 6.3±3.2, p=0.007), and shorter duration of MV among survivors (9.8±10.9 vs. 13.8±14.5, p=0.0002). There were no differences in ICU/60-day mortality or feeding intolerance rates between groups.Conclusion: In MV patients with circulatory shock, EEN, as compared to DEN, was associated with improved clinical outcomes. RCTs comparing the efficacy of EEN to DEN in MV patients with circulatory shock are warranted.
Failure of Inhibition Provides Transition From Population-Scale Computation to Focal Seizure
Authors: Smith Graham , Cowan Jack , Palmer Stephanie , Drongelen Wim 
Published: April 2022

Abstract
Focal seizures begin in the epileptogenic zone (EZ), a theoretical concept that evades practical identification and definition. Recently researchers have considered the more experimentally tractable “core” to elucidate the origins and behaviors of focal seizures. This millimeter-scale core is recruited by a wavefront of hyper-excitatory activity, in which inhibitory neurons fail to fire despite supra-threshold excitation. Herein we argue that the same failure of inhibition (FoI) as in the focal core may be critical to a theoretical understanding of the EZ. In contrast, most models of neural population activity have assumed that with increasing pre-synaptic input, population activity only increases. Two population models have recently made the critical step to account for FoI, with substantial success in modeling data from focal seizures. We provide a first-principles derivation from a single neuron model to a novel population model analogous to prior models. We show that our model fits as well or better than prior ad hoc FoI models on synthetic neural input-response data. We motivate the tristability of our model: having states of rest, functional activity, and pathological seizure. By analysing the transitions between these states, we can characterize seizure susceptibility and rescuability relative to functional excitability and stability. Thus, by relating a drug’s mechanism of action to our model parameters , our model can predict not only its nuanced effect on focal seizure, but also its effect on functional activity. The balance of these two effects is a key consideration in the clinical feasibility of an anti-epileptic drug.
Genetic regulators of cytokine responses in children from West Africa: the impact of BCG vaccination
Authors: Boahen* Collins K. , Moorlag* S.J.C.F.M. , Jensen Kristoffer Jarlov , Matzaraki Vasiliki , Fanucchi Stephanie , Monteiro Ivan , de Bree Charlotte , Fok Ezio T. , Mhlanga Musa , Joosten Leo A.B , Aaby Peter , Benn Christine Stabell , Netea Mihai G. , Kumar Vinod 
Published: April 2022

Abstract
Genetic variation is a key factor influencing cytokine production capacity, but which genetic loci regulate cytokine production before and after vaccination, particularly in African population is unknown. Here, we aimed to identify single-nucleotide polymorphisms (SNPs) controlling cytokine responses (cQTLs) after microbial stimulation in infants of West-African ancestry, comprising of low-birth-weight neonates randomized to BCG vaccine-at-birth (intervention) or to the usual delayed BCG (control). Genome-wide cytokine QTL-mapping revealed 12 independent cQTLs, of which the LINC01082-LINC00917 locus influenced more than half of the cytokine-stimulation pairs assessed. Furthermore, nine distinct cQTLs were found among infants randomized to BCG. Functional validation confirmed that several complement genes affect cytokine response after BCG vaccination. We observed limited overlap of common cQTLs between the West-African infants and cohorts of Western European individuals. These data reveal strong population-specific genetic effects on cytokine production and may indicate new opportunities for therapeutic intervention and vaccine development in African populations.
Sulfuricystis multivorans gen. nov., sp. nov. and Sulfuricystis thermophila sp. nov., facultatively autotropic sulfur-oxidizing bacteria isolated from a hot spring, and emended description of the genus Rugosibacter
Authors: Kojima Hisaya , Watanabe Miho , Miyata Naoyuki , Fukui Manabu 
Published: April 2022

Abstract
Strains J5BT and M52T are facultatively autotrophic sulfur-oxidizing bacterium isolated from a microbial mat from a hot spring. They were isolated and partially characterized in previous studies, as facultative anaerobes which use nitrate as electron acceptor. In this study, additional characterizations were made to determine their taxonomic status. In both strains, major cellular fatty acids were C16:1 (C16:1ω7c and/or C16:1ω6c) and C16:0. Their chemotihoautorophic growth was supported by thiosulfate and elemental sulfur. They used some organic acids as growth substrates. Their 16S rRNA gene sequences indicated the highest sequence identities to species in the family Sterolibacteriaceae, but the identities were 95% or lower. Values of average nucleotide identity and digital DNA–DNA hybridization between strains J5BT and M52T were 87.93% and 34.3%, respectively. Phylogenetic analysis of the 16S rRNA gene sequences indicated that these strains belong to the family Sterolibacteriaceae but not to any existing genera. On the basis of phenotypic and genomic characteristics, Sulfuricystis multivorans gen. nov. sp. nov., and Sulfuricystis thermophila sp. nov. are proposed, with type strains of J5BT (= BCRC 81386T = DSM 104688T = NBRC 112605T) and M52T (= BCRC 81387T = NBRC 114016T), respectively. An emended description of the genus Rugosibacter is also proposed, for its reclassification to the family Sterolibacteriaceae.
The impact of frailty on perioperative outcomes in patients receiving short-level posterior lumbar interbody fusion: a stepwise propensity score matching analysis
Authors: Lu Shibao , Cui Peng , Wang Peng , Wang Jinlin , Liu Xu , Kong Chao 
Published: April 2022

Abstract
Background There were many literatures reporting the impact of frailty on postoperative complications, however, studies according to stepwise propensity score matching (PSM) to offset the influence of age were limited.Methods We gathered data for all patients undergoing short-level posterior lumbar interbody fusion from January to December 2021. Accroding to Fried frailty phenotype, we segragated patients into three groups (not-frailty, pre-frailty, and frailty), then, stepwise propensity score matching for age, sex, American Society of Anesthesiologists (ASA) and fusion levels were performed to keep comparable clinical data between groups. Uni- and multivariate logistic regression were used to identify the impact of clinical demographics on postoperative complications.Results A total consecutive 559 patients with complete medical records were enrolled (237 males and 322 females; mean age 72.55 ± 5.86 years). After PS matching, we found pre-frail patients were more vulnerable to develop urinary retention ( p = 0.031 ) and surgical site infection ( p = 0.021 ) than non-frail patients. Meanwhile, longer length of stay (LOS), ambulation time and removal of urinary catheter time were observed in pre-frail patients. However, there was significant difference only in LOS between pre-frail and frail patients. Binary logistic regression revealed that C-reaction protein (CRP) and frailty status were independently associated with postoperative complications between non-frail and pre-frail patients, while only CRP was independently correlated with that of between pre-frail and frail patients.Conclusions In this study, after PS matching, we found higher rate of urinary retention and surgical site infection in pre-frail patients than that of non-frail patients. CRP was independently related to postoperative complications, while frailty status was only independently associated with postoperative complications between pre-frail and non-frail patients. And pre-frail/frail patients tended to have severer stress response than that of non-frail patients.
A Study on Impact of Emotional Intelligence of Retail Investors on Investment Decisions
Authors: awalakki Manjunath 
Published: April 2022

Abstract
A retail investor is a shareholder who buys stocks for his or her own personal account rather than for the benefit of the firm. Investment selections have become more important as a nation's job prospects and economic development have increased. Working people's capacity and desire to save and invest their money for returns has increased as a result of increased awareness of investment opportunities. Due to the liberalization of the Indian financial industry, there has been an amazing rise in the investment sector in terms of both volume and number of investors in India during the last decade. There is a flood of varied investment products with a plethora of possibilities to entice people to invest. In India, the number of regional stock exchanges has grown. Typically, investment choices are made using basic or technical approaches. However, in many circumstances, investors make investing decisions based on their emotions. This study analyses the impact of several elements such as biased representation, mental accounting, and risk aversion on the execution of an investment choice. Retail investment has experienced a meteoric rise in recent decades. The financial sector of every country is critical to the success and growth of other sectors of the economy, and this is especially true in developing economies. An effort was made to measure Emotion Regulation, Emotion Use or Motivation, Social Aptitudes, Loss Aversion, Status Quo, Endowment, Regret Aversion, Self-Care, and Investment Decisions. The regression findings revealed that Retail investors' Emotional Intelligence had a beneficial influence on investing decisions.
Qualitative study on the life dilemmas faced by long-term survivors of primary central nervous system germ cell tumors from a social ecological perspective
Authors: Bai Lan , zhou 周志欢 , Qin Huiying , Zhao Qinqin 
Published: April 2022

Abstract
Purpose
This study aimed to examine the life dilemmas of long-term survivors of central nervous system germ cell tumors based on the Social Ecological Model and provide a reference for targeted interventions.
Methods
A semi-structured interview was conducted on 14 survivors of central nervous system germ cell tumors. The questions were based on the Social Ecological Model. Data were transcribed and analyzed using Colaizzi’s method.
Results
Three themes were identified in this study. In the macro system, the physical and mental state of long-term survivors is affected by the disease and treatment, and the quality of life was poor. In the mezzo system, friendly work and community environments were included, but resources were scarce. Moreover, the support system in macro system was weak.
Conclusion
The social ecological system for long-term survivors of central nervous system germ cell tumors was poor. The society should pay attention to the survivors’ physical and mental health to promote better integration into society.
Prospective Changes in Diastolic Function in Patients with Rheumatoid Arthritis
Authors: Park Elizabeth , Ito Kazato , Iqbal Rabia , Amigues Isabelle , Bokhari Sabahat , Eyk Jennifer , Depender Christopher , Giles Jon T. , Bathon Joan 
Published: April 2022

Abstract
Background: Diastolic dysfunction (DD) is more prevalent in patients with rheumatoid arthritis (RA) compared to the general population. However, its evolution over time and its significant clinical predictors, remain uncharacterized. We report on baseline and prospective changes in diastolic function and its associated RA and cardiovascular (CV) predictors.Methods: In this study, 158 RA patients without clinical CV disease (CVD) were enrolled and followed up at 4 to 6 years, undergoing baseline and followup echocardiography to assess for DD, as well as extensive characterization of RA disease activity and CV risk factors. Novel measures of myocardial inflammation and perfusion were obtained at baseline only. Using baseline and followup composite DD (E/e’, Left Atrial Volume Index(LAVI) or peak Tricuspid Regurgitation(TR) velocity; ≥ 1 in top 25%) as the outcome, multivariable regression models were constructed to identify predictors of DD.Results: DD was prevalent in RA patients without clinical heart failure (HF) (40.7% at baseline), and significantly progressed on followup (to 57.9%). Baseline composite DD was associated with baseline RA disease activity (Clinical Disease Activity Index;CDAI) (OR 1.39; 95% CI 1.02-1.90; p=0.034). Several individual diastolic parameters (baseline E/e' and LAVI) were associated with troponin-I and Brain Natiuretic Peptide (BNP). Baseline and followup composite DD, however, were not associated with myocardial inflammation, myocardial microvascular dysfunction, or subclinical atherosclerosis.Conclusions: DD is prevalent in RA patients without clinical HF and increases to >50% over time. Higher RA disease activity at baseline predicted baseline composite DD. Future longitudinal studies should explore whether adverse changes in diastolic function lead to clinical HF and are attenuated by disease-modifying antirheumatic drugs (DMARDs).
Etiology Model for Elevated Histamine Levels Driving High Reactogenicity Vaccines (including COVID-19) Associated Menstrual Adverse Events
Authors: Ricke Darrell O. 
Published: April 2022

Abstract
Introduction: Some women are experiencing menstrual changes, including altered menstrual duration, volume (heavier bleeding), increased dysmenorrhea, and worsened premenstrual syndrome (PMS) following Coronavirus disease 2019 (COVID-19) spike vaccinations. Appreciation of these as temporal adverse events associated with COVID-19 spike vaccinations was slow to develop. The etiology of these menstrual adverse events associated with COVID-19 spike vaccination remains unknown. Methods: The United States Department of Health and Human Services Vaccine Adverse Event Reporting System (VAERS) database was data mined for data reported adverse events affecting menstrual cycles by vaccine type.Hypothesis: This article proposes the hypothesis that vaccinations can induce a temporary surge in histamine levels immediately post vaccination as part of the innate immune response. Increasing histamine levels is known to increase estrogen levels. Further, it is proposed that this temporary surge in histamine levels causes temporary Histamine Intolerance in some women and causes the menstrual adverse events temporally associated with vaccinations.Conclusion: Prophylactic and therapeutic treatment of vaccinees with diamine oxidase and/or specific antihistamines may reduce the incidence rate and/or severity of menstrual adverse events associated with vaccines with high reactogenicity, including SARS-CoV-2 vaccines and boosters. This model predicts menstrual associated adverse events incidence levels correspond to vaccine reactogenicity.
Oxidative stress-induced by 30 days of mercury exposure triggers acceleration of hypertension development in prehypertensive young SHR rats
Authors: Ronchetti Graziele Zandominegue , Simões Maylla Ronacher , Schereider Ingridy Reinholz Grafites , Leal Marcos André Soares , Peçanha Giulia Alessandra Wiggers , Padilha Alessandra Simão , Vassallo Dalton Valentim 
Published: April 2022

Abstract
Mercury is considered a risk factor for hypertension development and other cardiovascular diseases. Objectives: We investigated whether the effects of mercury exposure on hemodynamic parameters of young Wistar and prehypertensive SHR animals might influence the time course of hypertension development. Main methods: Young (4 weeks) male Wistar and SHR rats were randomly assigned to 4 groups: untreated Wistar rats (Wistar Ct), Wistar rats exposed to mercury chloride (Wistar Hg); untreated SHR rats (SHR Ct) and SHR rats exposed to mercury chloride (SHR Hg) for 30 days. Non-invasive and invasive arterial pressure were measured to investigate pressure reactivity; nitrite/nitrate levels, ACE activity, and lipid peroxidation were measured in plasma. Results: Systolic blood pressure (SBP) of Wistar groups did not change but increased in SHR from the second week to the last one. Hg exposure accelerated the increase of SBP in SHR rats. L-NAME administration increased SBP and diastolic blood pressure (DBP) in all groups, but this increase was smaller in SHR animals exposed to Hg. A decrease of plasma nitrite and nitrate in SHR Hg group suggested that mercury reduced NO bioavailability. Tempol reduced blood pressure, suggesting that the superoxide anion played a role in the marked increase of this parameter. These findings provide evidence that the effects of exposure to Hg might trigger mechanisms to accelerate the hypertension development including NO bioavailability reduction. Therefore, mercury might enhance the risk to cardiovascular disorders not only for adults but also for pre-disposed young ones enhancing hypertension development.