bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioengineered 3D microfibrous‐matrix modulates osteopontin release from MSCs and facilitates the expansion of hematopoietic stem cells
Authors: Nandakumar Niji , Mohan Malini , Thilakan Akhil T. , Sidharthan Hridhya K. , Ramu Janarthanan , Sharma Deepti , Nair Shantikumar V. , Sathy Binulal N. 
Published: July 2022

AbstractThe osteopontin (OPN) released from mesenchymal stem cells (MSCs) undergoing lineage differentiation can negatively influence the expansion of hematopoietic stem cells (HSCs) in coculture systems developed for expanding HSCs. Therefore, minimizing the amount of OPN in the coculture system is important for the successful ex vivo expansion of HSCs. Toward this goal, a bioengineered three dimensional (3D) microfibrous‐matrix that can maintain MSCs in less OPN‐releasing conditions has been developed, and its influence on the expansion of HSCs has been studied. The newly developed 3D matrix significantly decreased the release of OPN, depending on the MSC culture conditions used during the priming period before HSC seeding. The culture system with the lowest amount of OPN facilitated a more than 24‐fold increase in HSC number in 1 week time period. Interestingly, the viability of expanded cells and the CD34+ pure population of HSCs were found to be the highest in the low OPN‐containing system. Therefore, bioengineered microfibrous 3D matrices seeded with MSCs, primed under suitable culture conditions, can be an improved ex vivo expansion system for HSC culture.
Dual cytoplasmic‐peroxisomal engineering for high‐yield production of sesquiterpene α‐humulene in Yarrowia lipolytica
Authors: Guo Qi , Li Ya‐Wen , Yan Fang , Li Ke , Wang Yue‐Tong , Ye Chao , Shi Tian‐Qiong , Huang He 
Published: July 2022

AbstractThe sesquiterpene α‐humulene is an important plant natural product, which has been used in the pharmaceutical industry due to its anti‐inflammatory and anticancer activities. Although phytoextraction and chemical synthesis have previously been applied in α‐humulene production, the low efficiency and high costs limit the development. In this study, Yarrowia lipolytica was engineered as the robust cell factory for sustainable α‐humulene production. First, a chassis with high α‐humulene output in the cytoplasm was constructed by integrating α‐humulene synthases with high catalytic activity, optimizing the flux of mevalonate and acetyl‐CoA pathways. Subsequently, the strategy of dual cytoplasmic‐peroxisomal engineering was adopted in Y. lipolytica; the best strain GQ3006 generated by introducing 31 copies of 12 different genes could produce 2280.3± 38.2 mg/l (98.7 ± 4.2 mg/g dry cell weight) α‐humulene, a 100‐fold improvement relative to the baseline strain. To further improve the titer, a novel strategy for downregulation of squalene biosynthesis based on Cu2+‐repressible promoters was firstly established, which significantly improved the α‐humulene titer by 54.2% to 3516.6 ± 34.3 mg/l. Finally, the engineered strain could produce 21.7 g/l α‐humulene in a 5‐L bioreactor, 6.8‐fold higher than the highest α‐humulene titer reported before this study. Overall, system metabolic engineering strategies used in this study provide a valuable reference for the highly sustainable production of terpenoids in Y. lipolytica.
Layered similarity detection for programming plagiarism and collusion on weekly assessments
Authors: Karnalim Oscar , Simon  , Chivers William 
Published: July 2022

AbstractWhen weekly programming assessments are used, it is often the case that some of them are either trivial or strongly directed. Common code similarity detectors are not particularly helpful with such assessments: some potential instances of misconduct are not selected for manual investigation as all submissions are expected to be similar and it is not feasible to check them all. Several dedicated similarity detectors have been developed to work with such assessments, but the experience is required to determine when to use them. This paper presents a similarity detector that works on many kinds of weekly assessments. It combines three‐layered types of similarity so that even within a set of highly similar submissions, program pairs are still sorted according to their levels of similarity. Our similarity detector is more effective than JPlag in distinguishing similar programs and helping to identify plagiarism and collusion. The similarity detector is slower than JPlag, but the longer execution time is partly offset by some optimization that has no negative impact on the effectiveness. As weekly assessments seldom entail large submissions, the execution time does not appear to be a barrier to use.
Assembly of Two‐Dimensional DNA Arrays Could Influence the Formation of Their Component Tiles
Authors: Paluzzi Victoria E. , Zhang Cuizheng , Mao Chengde 
Published: July 2022

AbstractTile‐based DNA self‐assembly is a powerful approach for nano‐constructions. In this approach, individual DNA single strands first assemble into well‐defined structural tiles, which, then, further associate with each other into final nanostructures. It is a general assumption that the lower‐level structures (tiles) determine the higher‐level, final structures. In this study, we present concrete experimental data to show that higher‐level structures could, at least in the current example, also impact on the formation of lower‐level structures. This study prompts questions such as: how general is this phenomenon in programmed DNA self‐assembly and can we turn it into a useful tool for fine tuning DNA self‐assembly?
Sex differences on outcomes following left atrial appendage occlusion in atrial fibrillation: A systematic review and meta‐analysis
Authors: Zhu Yuansong , Sasmita Bryan Richard , Xue Yuzhou , Jiang Yi , Huang Bi , Luo Suxin 
Published: July 2022

AbstractThere is a lack of sufficient data on sex‐related differences in outcomes of nonvalvular atrial fibrillation (AF) patients following left atrial appendage occlusion (LAAO). We conducted a meta‐analysis to investigate the procedural complications and long‐term outcomes after LAAO in women versus men. We screened Medline, EMBASE, Cochrane Center Register of Controlled Trials, and Clinical Trials.gov. The inclusion criteria were studies targeting the sex‐related differences in outcomes in nonvalvular AF patients treated by LAAO. Procedural endpoints of interest included success rate, pericardial complications, major bleeding, and vascular complications during hospitalization. Long‐term outcomes included all‐cause mortality and ischemic stroke during follow‐up. Studies that merely considered sex in the subgroup analysis were not included. Six observational studies with a total of 64,035 patients were identified. The procedural success rates did not differ between sexes (odds ratio [OR]: 0.98, 95% confidence interval [CI]: 0.89–1.09, p = 0.77), while women experienced more pericardial complications (OR: 1.78, 95% CI: 1.58–2.01, p < 0.00001), major bleedings (OR: 2.04, 95% CI: 1.75–2.39, p < 0.00001), and vascular complications (OR: 1.75, 95% CI: 1.41–2.17, p < 0.00001) than men. The sensitivity analysis performed by removing the largest study showed good stability. The long‐term mortality and stroke rates did not differ between women and men in either the 1‐year subgroup or the 2‐year subgroup. In conclusion, despite comparable procedural success rates, women have a significantly higher incidence of pericardial complications, major bleeding, and vascular complications following LAAO. The long‐term mortality and stroke rates do not differ between the sexes.
Local versus general anesthesia for endovascular aneurysm repair in ruptured abdominal aortic aneurysm: A systematic review and meta‐analysis
Authors: Lei Jiahao , Pu Hongji , Wu Zhaoyu , Huang Qun , Yang Xinrui , Liu Guang , Lu Xinwu 
Published: July 2022

AbstractObjectives/BackgroundIn the endovascular treatment of ruptured abdominal aortic aneurysm (RAAA), there is no effective evidence to show preference for a specific anesthetic option. A meta‐analysis was conducted to assess the result of different anesthesia in endovascular aneurysm repair (EVAR) of RAAA.MethodsRandomized controlled trials (RCTs) and cohort studies were searched in PubMed, Embase, Ovid, and the Cochrane Library. Newcastle‐Ottawa Scale and the Cochrane Risk of Bias Tool were applied to evaluate the quality of cohort studies and RCTs, respectively. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to express differences for primary and secondary outcomes. Subgroup analyses and sensitivity analyses were applied in the primary outcome to illustrate the results further. Significance was set at p < 0.05. Random‐effects models were used considering limited research regardless of I2 < 50%.ResultsTen cohort studies were included in this meta‐analysis. Perioperative mortality was presented as the primary outcome by analyzing eight of these research. Among the included patients, local anesthesia (LA) was considered as a better choice considering perioperative mortality (n = 156/902) rather than general anesthesia (n = 907/3434) with significant difference (OR: 0.49; 95% CI: 0.35−0.67; p < 0.00001; I2 = 42%). However, no significant difference was found in the secondary outcome: the complication rate, ICU admission rate, postoperative morbidity of pneumonia, myocardial infarction, leg ischemia, and wound complication.ConclusionsThere exists some evidence in this review that LA appears to improve perioperative mortality, especially in hemodynamically stable patients and should be recommended for patients undergoing EVAR with RAAA when appropriate.
Sr Surface Enrichment in Solid Oxide Cells – Approaching the Limits of EDX Analysis by Multivariate Statistical Analysis and Simulations
Authors: Türk Hanna , Götsch Thomas , Schmidt Franz‐Philipp , Hammud Adnan , Ivanov Danail , de Haart L. G. J. (Bert) , Vinke Izaak C. , Eichel Rüdiger‐A. , Schlögl Robert , Reuter Karsten , Knop‐Gericke Axel , Lunkenbein Thomas , Scheurer Christoph 
Published: July 2022

AbstractIn solid oxide cells, Sr segregation has been correlated with degradation. Yet, the atomistic mechanism remains unknown. Here we begin to localize the origin of Sr surface nucleation by combining force field based simulations, energy dispersive X‐ray spectroscopy (EDX), and multi‐variate statistical analysis. We find increased ion mobility in the complexion between yttria‐stabilized zirconia and strontium‐doped lanthanum manganite. Furthermore, we developed a robust and automated routine to detect localized nucleation seeds of Sr at the complexion surface. This hints at a mechanism originating at the complexion and requires in‐depth studies at the atomistic level, where the developed routine can be beneficial for analyzing large hyperspectral EDX datasets.
An Enzyme Cascade Reaction for the Recovery of Hydroxytyrosol Dervatives from Olive Mill Wastewater
Authors: Terholsen Henrik , Kaur Jasmin , Kaloudis Nikolaos , Staudt Amanda , Pavlidis Ioannis V. , Bornscheuer Uwe T. 
Published: July 2022

AbstractThe valorization of olive mill wastewaters (OMWW), a by‐product of the olive milling, is getting rising attention. Lipophilization of the main phenolic compound 3‐hydroxytyrosol (HT) could facilitate its extraction. An immobilized variant of the promiscuous hydrolase/acyltransferase from Pyrobaculum calidifontis VA1 (PestE) was used to perform acetylation in water using ethyl acetate as acyl donor. PestE was used in a segmented flow setting to allow continuous operation. Additionally, HT precursors were made accessible by pretreatment with almond β‐glucosidase and the hydrolytic activity of PestE_I208A_L209F_N288A.
Use of a Scattered Light Sensor for Monitoring the Dispersed Surface in Crystallization
Authors: Schmitt Lukas , Meyer Conrad , Schorz Stefan , Manser Steffen , Scholl Stephan , Rädle and Matthias 
Published: July 2022

AbstractThe specific surface area of the dispersed phase in suspensions, emulsions, bubble columns and aerosols plays a decisive role in the increasement of heat and mass transfer processes. This has a direct effect on the space‐time yield in large‐scale chemical/process engineering production plants. Initial investigations show that an easy‐to‐install optical backscatter sensor outputs the dispersed surface area as a direct primary signal under certain boundary conditions. The article describes the construction, the mode of operation and first investigations applying of this new sensor. The sensor works even in high concentrated suspensions and emulsions, where conventional nephelometry already fails. Trends and limitations found so far for the sensor, which can be used in‐line in batch and continuously operated crystallizers, even in harsh production environments and in potentially explosive zones, are shown.
Experimental methods in chemical engineering: Pressure
Authors: Wu Kaiqiao , Galli Federico , de Tommaso Jacopo , Patience Gregory S. , van Ommen J. Ruud 
Published: July 2022

AbstractPressure and temperature are the most important state variables for monitoring physicochemical processes to detect deviations that might lead to explosions and to verify levels, flow rate, and solids/gas hold‐up. Pressure fluctuations in multi‐phase systems identify regime changes and flow anomalies. Pressure signals are the first indicator of a process upset and are tied into distributed control systems (DCS) to sound alarms when they drift to high or low and activate safety interlocks in the case of high, high‐high, low, and low‐low conditions. To maximize the information, it requires that pressure gauges (transducers) are installed and calibrated precisely. Pressure measuring devices include manometers, aneroid devices like bellows and Bourdon gauges, and electronic instruments—piezoresistive, piezoelectric, and capacitive. The electronic elements have the advantage of higher precision and faster response times to measure fluctuations. The Bourdon gauges are standard equipment for pressure regulators and are mounted on the exterior of vessels and pipes to facilitate visual inspections. Over 2 million articles indexed by the Web of Science Core collection mention pressure, and in 2021, chemical engineering ranks had over 7000 articles—only multidisciplinary material sciences and energy and fuels had more. A bibliometric analysis identified five research clusters: temperature, combustion, and kinetics; separation, membranes, and energy efficiency; carbon dioxide (capture and storage), water, and thermodynamics; methane, adsorption, and transport phenomena (e.g., diffusion and permeability); and modelling, optimization, and computational fluid dynamics (CFD).
Mixed neuroendocrine–non‐neuroendocrine neoplasm arising from long‐segment Barrettʼs esophagus showing exceptionally aggressive clinical behavior
Authors: Miyaguchi Kazuya , Kawasaki Tomonori , Tashima Tomoaki , Ryozawa Shomei 
Published: July 2022

AbstractBackgroundThere is only one report of Barrett's esophagus (BE) with mixed neuroendocrine–non‐neuroendocrine neoplasm (MiNEN). Herein, for the first time, we present a case with an aggressive esophageal MiNEN, as well as with both primary MiNEN and conventional adenocarcinoma, arising in BE.CaseA 68‐year‐old woman had been diagnosed with 0‐IIa type adenocarcinoma in the background of long‐segment BE, 45 months earlier. She underwent endoscopic submucosal dissection (ESD) and the pathological diagnosis was tubular adenocarcinoma, well‐differentiated, with slight submucosal invasion. There was no lymphovascular invasion and the margins were intact. The upper esophagogastroduodenoscopy conducted the year after ESD showed no residual or recurrent cancer. However, she was subsequently followed up at another hospital, and endoscopy was not performed after the second year. She was urgently transported to our hospital due to buttock pain in the ninth month of the fourth year. A computed tomography (CT) of the head showed multiple cerebral metastases and positron emission tomography–CT revealed numerous osseous and nodal involvements. We performed upper endoscopy and detected type 3 esophageal tumor. Multiple biopsy specimens histopathologically contained invasive neoplasm composed of neuroendocrine carcinoma (NEC) and adenocarcinoma, moderately to poorly differentiated. The NEC element showed diffuse proliferation of primitive cancer cells possessing fine‐granular cytoplasm and nuclei with prominent nucleoli, whereas the adenocarcinoma component had tubules or nested growth of basophilic cells. Immunohistochemically, the NEC cells were diffusely positive for synaptophysin, with focal expressions of INSM1, chromogranin A and NCAM, whereas the adenocarcinoma cells were mostly negative for these NE markers. The Ki67 index was 90% at the hot spots in both types. The patient died 3.5 months after the biopsy‐based histological diagnosis.ConclusionAppropriate therapy according to the guidelines and/or meticulous clinical follow‐up based on periodic endoscopy as well as a full physical examination are essential, from a proactive perspective, for early diagnosis of secondary aggressive cancers after ESD.
The influence of proton pump inhibitors on tissue attachment around teeth and dental implants: A scoping review
Authors: Chawla Bhavneet K. , Cohen Robert E. , Stellrecht Elizabeth M. , Yerke Lisa M. 
Published: July 2022

AbstractObjectivesProton pump inhibitors, such as omeprazole and pantoprazole, are frequently prescribed for the treatment of acid reflux. However, those medications have been shown to affect a variety of physiologic processes, including bone homeostasis and the gastrointestinal microbiome. The objective of this study was to assess the relationship between proton pump inhibitors and attachment levels around teeth and dental implants. A scoping review was performed to assess the extent and quality of the relevant literature.Materials and MethodsWe used the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses extension for scoping reviews (PRISMA‐ScR) and searched four relevant biomedical literature databases in addition to the grey literature. Keywords in the title and abstract fields, and subject headings for proton pump inhibitors, teeth, and dental implants were included as search terms.ResultsOverall search results identified 791 publications which, after applying the inclusion and exclusion criteria, yielded 27 publications that were further analyzed for relevance and quality of scientific evidence. The majority of eligible publications were retrospective cohort studies. Following critical analysis, 13 publications, including six abstracts, were used to assess the effect of proton pump inhibitors on tissue attachment around teeth and dental implants.ConclusionsThere are few high‐quality studies describing the effect of proton pump inhibitors on tissue attachment around teeth and dental implants. Nevertheless, among the included papers with the fewest confounding factors, there was a positive relationship between proton pump inhibitors and soft tissue attachment levels around teeth, and a predominantly negative but variable effect of proton pump inhibitors on the bone level around dental implants. Additional well‐controlled prospective studies are required to fully elucidate those relationships.
Pain intensity and salivary α‐amylase activity in patients following mandibular third molar surgery
Authors: Surin Wanvipa , Chatiketu Piyanart , Hutachok Nuntouchaporn , Srichairatanakool Somdet , Chatupos Vuttinun 
Published: July 2022

AbstractObjectiveWe aimed to compare the levels of pain and salivary α‐amylase (SAA) in patients before and after mandibular third molar surgery.Materials and MethodsPatients were divided into asymptomatic and symptomatic groups and were then identified by the analgesic drug taken throughout the 2‐week study. The visual analog scale (VAS) was employed to evaluate the severity of pain experienced by a given subject before treatment, when the anesthetic wore off, in the morning, and at night for a period of 1 week. Saliva was collected from the mouth floor of the subjects and the levels of SAA activity were measured at indicated times.ResultsThe levels of postoperative pain were higher than those of pretreatment pain (p < 0.05), but were not necessarily different between the two groups. The pain levels were positively correlated with SAA activities in both groups (p < 0.05). There was no difference between the number of analgesics taken by the two groups and the postoperative complications observed during the study. A significant correlation was observed between the VAS pain scale and SAA activities.ConclusionSAA would be a simple effective biomarker for the objective assessment of pain intensity in patients who have undergone mandibular third molar surgery.
β‐Aminosulfonyl Fluorides via Water‐Accelerated N‐Heterocyclic Carbene Catalysis
Authors: Park Jin Hyun , Lee Sun Bu , Koo Byeong Jun , Bae Han Yong 
Published: July 2022

AbstractHerein, a water‐accelerated, N‐heterocyclic carbene (NHC)‐catalyzed aza‐Michael addition reaction was reported to access β‐aminosulfonyl fluorides, which are key hubs of the sulfur(VI) fluoride exchange (SuFEx) reaction. As a crucial reaction medium, water considerably enhanced the reaction rate with excellent chemo‐ and site‐selectivity (up to >99 : 1) compared to conventional solvents. In addition, the late‐stage ligation of bioactive molecules with the aliphatic β‐amino SuFEx hub was demonstrated. Mechanistic studies on experimental, analytical, and computational approaches support noncovalent activation over NHC catalysis “on‐water”.
Emerging methodologies in stability and optimization problems of learning‐based nonlinear model predictive control: A survey
Authors: Meng Fei , Shen Xuyu , Karimi Hamid Reza 
Published: July 2022

AbstractSince last 40 years, the theory and technology of model predictive control (MPC) have been developed rapidly. However, nonlinear MPC still faces difficulties such as high online computational complexity and inability to accurately model the system. In order to improve or solve the problems mentioned above of MPC, recent researches have deepened the learning‐based control. The learned method can model unknown or highly uncertain nonlinearities. And the emergence of efficient algorithms has greatly improved the feasibility of computing. Stability is at the heart of control design. Learning‐based nonlinear model predictive control (LB‐NMPC) has achieved systematic research results in the past 10 years. But the stability of LB‐NMPC is still an open question that has not been fully addressed in the literature. This review mainly summarizes the latest research progress of LB‐NMPC. More specifically, the uncertainty and online optimization problems of the considered systems are investigated mainly focusing on the use of learning techniques. At the same time, the research hotspots such as the control stability and constraint satisfaction of LB‐NMPC are briefly discussed. Finally, the application of LB‐NMPC technology in integrated circuits, path tracking control, and other fields is reviewed, which provides a reference for the research and application of LB‐NMPC.