bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plpp3, a novel regulator of pluripotency exit and endodermal differentiation of mouse embryonic stem cells
Authors: Montané-Romero Martha E. , Martínez-Silva Ana V. , Poot-Hernández Augusto C. , Escalante-Alcalde Diana 
Published: December 2022

ABSTRACT
In recent decades, study of the actions of bioactive lipids such as lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) has increased since they are involved in regulating many processes, including self-renewal of embryonic stem cells, embryo development and cancer. Phospholipid phosphatase type 3 (PLPP3) has been shown to be a key player in regulating the balance of these lipids and, in consequence, their signaling. Different lines of evidence suggest that PLPP3 could play a role in endoderm development. To approach this hypothesis, we used mouse embryonic stem cells (ESC) as a model to study Plpp3 function in self-renewal and the transition towards differentiation. We found that lack of PLPP3 mainly affects endoderm formation during differentiation of suspension-formed embryoid bodies. PLPP3-deficient ESC strongly decrease the amount of FOXA2-expressing cells and fail to properly downregulate the expression of pluripotency factors when subjected to an endoderm-directed differentiation protocol. Impaired endoderm differentiation correlated with a transient reduction in nuclear localization of YAP1. These phenotypes were rescued by transiently restoring the expression of catalytically active hPLPP3. In conclusion, PLPP3 plays a role in downregulating pluripotency-associated factors and in endodermal differentiation. PLPP3 regulates proper lipid/YAP1 signaling required for endodermal differentiation.
What Do We Know about Frame Running? A Narrative Review
Authors: Voltolini Lucas de Assis , De Araújo Pedro Henrique , Antunes Diego , Lima Gabriel Benedito , de Lucas Ricardo Dantas , Fischer Gabriela 
Published: December 2022

AbstractThis narrative review aims to provide a general overview of the literature about frame running, which is a recent modality of Para-Athletics. Frame running is practiced by using a tricycle without pedals called PETRA RaceRunner, by people with moderate to severe cerebral palsy and other lower limb functional limitations. Briefly, the movement pattern is very similar to walking and running. This review includes studies from scientific databases and content of official sports web sites by using the keywords “framerunning,” “racerunning,” and “petra racerunning.” According to our search, this narrative review highlighted three themes involving the practice of frame running, namely health and quality of life, sports classification, and training and testing in the frame running context.
Inventory of Terrestrial Orchids in the Baturraden Botanical Garden of Central Java
Authors: Astuti Sutan Nur Chamida Tri , Aisiah Siti , Kurniawan Ardyan Pramudya 
Published: December 2022

Baturraden Botanical Garden located on the slopes of Mount Slamet in the south has a high enough humidity so it is ideal for plant growth, especially orchids. As an ex-situ plant conservation area, the Baturraden Botanical Garden plays an important role in preserving the diversity of orchids in it. The purpose of this study was to find out the types and descriptions of terrestrial orchids in the Baturraden Botanical Garden area of Central Java. The method used in this study is a 400 m line transect method with an inter-point distance of 100 m each and data collection is carried out at 10 m each on the right and left of the line transect. Hasil research shows that there are 13 species derived from 10 genus of soil orchids. The types that have been found are Goodyera rubicunda (Blume) Lindl., Calanthe pulchra (Blume) Lindl., Spathoglottis plicata Blume, Cryptostylis javanica J.J.Sm., Appendicula alba Blume, Phaius callosus (Blume) Lindl., Lepidogyne longifolia Blume, Tainia elongata J.J.Sm., Goodyera colorata Blume, Malaxis sp. Soland. ex Sw., Goodyera sp. R. Br., Goodyera procera (Ker-Gawl.) Hook., and Plocoglottis sp. Blume.
Species Diversity and Dominance of the Aranaemorphae Sub-Order in the 90-Day-Old Rice Generative Phase
Authors: Putra Ichsan Luqmana Indra , Pertiwi Pelangi Mayang Sukma 
Published: December 2022

Spiders have an important role in ecosystems, in particular agricultural ecosystems. Spiders play the role of natural enemies capable of helping farmers by eating insect pests. The purpose of this study was to identify the type of Sub-Order Aranaemorphae and calculate the level of diversity and dominance of the type of Sub-Order Aranaemorphae in the generative phase of rice aged 90 days. The study was conducted in April-September 2022 in the rice field area of Tamanan Village. The determination of the research location was carried out using the purposive sampling method with the criteria of a rice field area of 2500 m2 and the age of rice plants ranging from 90 days. Sampling was carried out on 9 plots with a plot size of 21 x 4 m2 each. Sampling was carried out by hand picking and indirect methods with sweep nets, pitfall traps, and yellow pan traps. The species of Sub-Order Aranaemorphae found in this study were 12 species, namely Oxyopes sp., O. lineatipes, O. javanus, Atypena formosana, Tetragnatha vermiformis, Pardosa pseudoannulata, Lycosa sp., L. pseudoannulata, Lycosa sp. 1, Araneus inustus, Clubiona sp. and Thomisus Sp. The conclusion of this study were the diversity index value (H') of the Aranaemorphae Sub-Order obtained in this study was 2.133 with a moderate category, and the dominance index value (D) of the Aranaemorphae Sub-Order obtained was 0.1785 with a low category. The total number of spider individuals obtained was 173 individuals.
Morphometric Variations of Chalcorana chalconota (Schlegel, 1837) in Four Populations in Jatimulyo Kulon Progo
Authors: Hanifuddin Afnan Saud , Aini Khuratul , Suwartiningsih Nurul 
Published: December 2022

Jatimulyo is an ecotourism area consisting of several areas, each of which has a different height and abiotic factors. This can affect the morphometric character of frogs including Chalcorana chalconota. Research on specific morphometric variations of Chalcorana chalconota in Jatimulyo ecotourism has never been conducted. The purpose of this study was to analyze the distinguishing character of four frog populations as well as analyze the morphometric variation of the Chalcorana chalconota frog in Jatimulyo ecotourism. The research samples were taken using the visual encounter survey method and direct collection in the field in all four populations. Morphometric measurements use 32 characters. The measurement data obtained were analyzed using the Kruskall-Wallis test followed by UPGMA to see dissimilarity using the MVSP 3.1 program. The results showed that of the 32 characters observed and analyzed, there were nine characters that had a p<0.05 value which means it is significant to distinguish the four populations. The nine characters are (HW), (TD), (MFE), (MBE), (HAL), (LAL), (FOL), (IN), (MTL). LK, DT, JMMD, JMMB, JIN, PMD, PBR, PTJ4, PTM. The frog Chalcorana chalconota population of Kembang Soka has a high morphometric similarity with the population of Kedung Pedut. The Mudal River Population and the Bull Kedung Population have a considerable morphometric similarity with the Soka Flower and Pedut Flower Populations. This study can be concluded that the morphometric variation of Chalcorana chalconota in Jatimulyo ecotourism is influenced by altitude factors.
Case-control investigation of invasive Salmonella disease in Malawi reveals no evidence of environmental or animal transmission of invasive strains, and supports human to human transmission
Authors: Koolman Leonard , Prakash Reenesh , Diness Yohane , Msefula Chisomo , Nyirenda Tonney S. , Olgemoeller Franziska , Wigley Paul , Perez-Sepulveda Blanca , Hinton Jay C. D. , Owen Siân V. , Feasey Nicholas A. , Ashton Philip M. , Gordon Melita A. 
Published: December 2022

Background
Invasive Salmonella infections cause significant morbidity and mortality in Sub-Saharan Africa. However, the routes of transmission are uncertain. We conducted a case-control study of index-case and geographically-matched control households in Blantyre, Malawi, sampling Salmonella isolates from index cases, healthy people, animals, and the household environment.

Methodology
Sixty index cases of human invasive Salmonella infection were recruited (March 2015-Oct 2016). Twenty-eight invasive Non-Typhoidal Salmonella (iNTS) disease and 32 typhoid patients consented to household sampling. Each index-case household was geographically matched to a control household. Extensive microbiological sampling included stool sampling from healthy household members, stool or rectal swabs from household-associated animals and boot-sock sampling of the household environment.

Findings
1203 samples from 120 households, yielded 43 non-Typhoidal Salmonella (NTS) isolates from 25 households (overall sample positivity 3.6%). In the 28 iNTS patients, disease was caused by 3 STs of Salmonella Typhimurium, mainly ST313. In contrast, the isolates from households spanned 15 sequence types (STs). Two S. Typhimurium isolates from index cases closely matched isolates from their respective asymptomatic household members (2 and 3 SNP differences respectively). Despite the recovery of a diverse range of NTS, there was no overlap between the STs causing iNTS disease with any environmental or animal isolates.

Conclusions
The finding of NTS strains from index cases that matched household members, coupled with lack of related animal or environmental isolates, supports a hypothesis of human to human transmission of iNTS infections in the household. The breadth of NTS strains found in animals and the household environment demonstrated the robustness of NTS sampling and culture methodology, and suggests a diverse ecology of Salmonella in this setting. Healthy typhoid (S. Typhi) carrier state was not detected. The lack of S. Typhi isolates from the household environment suggests that further methodological development is needed to culture S. Typhi from the environment.
Cytochrome P450 2D6 (CYP2D6) and glucose-6-phosphate dehydrogenase (G6PD) genetic variations in Thai vivax malaria patients: Implications for 8-aminoquinoline radical cure
Authors: Chamchoy Kamonwan , Sudsumrit Sirapapha , Thita Thanyapit , Krudsood Srivicha , Patrapuvich Rapatbhorn , Boonyuen Usa 
Published: December 2022

Background
Primaquine and tafenoquine are the only licensed drugs that effectively kill the hypnozoite stage and are used to prevent Plasmodium vivax malaria relapse. However, both primaquine and tafenoquine can cause acute hemolysis in glucose-6-phosphate dehydrogenase (G6PD)-deficient people with varying degrees of severity depending on G6PD variants. Additionally, primaquine efficacy against malaria parasites was decreased in individuals with impaired cytochrome P450 2D6 (CYP2D6) activity due to genetic polymorphisms. This study aimed to characterize G6PD and CYP2D6 genetic variations in vivax malaria patients from Yala province, a malaria-endemic area along the Thai–Malaysian border, and determine the biochemical properties of identified G6PD variants.

Methodology/Principle findings
Multiplexed high-resolution melting assay and DNA sequencing detected five G6PD variants, including G6PD Kaiping, G6PD Vanua Lava, G6PD Coimbra, G6PD Mahidol, and G6PD Kerala-Kalyan. Biochemical and structural characterization revealed that G6PD Coimbra markedly reduced catalytic activity and structural stability, indicating a high susceptibility to drug-induced hemolysis. While Kerala-Kalyan had minor effects, it is possible to develop mild adverse effects when receiving radical treatment. CYP2D6 genotyping was performed using long-range PCR and DNA sequencing, and the phenotypes were predicted using the combination of allelic variants. Decreased and no-function alleles were detected at frequencies of 53.4% and 14.2%, respectively. The most common alleles were CYP2D6*36+*10 (25.6%), *10 (23.9%), and *1 (22.2%). Additionally, 51.1% of the intermediate metabolizers showed CYP2D6*10/*36+*10 as the predominant genotype (15.9%).

Conclusions/Significance
Our findings provide insights about genetic variations of G6PD and CYP2D6 in 88 vivax malaria patients from Yala, which may influence the safety and effectiveness of radical treatment. Optimization of 8-aminoquinoline administration may be required for safe and effective treatment in the studied population, which could be a significant challenge in achieving the goal of eliminating malaria.
Assessment of magnitude and spectrum of cardiovascular disease admissions and outcomes in Saint Paul Hospital Millennium Medical College, Addis Ababa: A retrospective study
Authors: Mengistu Mekoya D. , Benti Henok 
Published: December 2022

BackgroundCardiovascular diseases(CVD) remain the leading cause of death in the world and over 80% of all cardiovascular-related deaths occur in low and middle income countries. Ethiopia is in epidemiologic transition from predominantly infectious diseases to non-communicable diseases and the CVD is a major public health challenge.MethodsThe aim of this study was to assess the magnitude and spectrum of cardiovascular admission and its outcomes among medical patients admitted to both Medical Ward and ICU of St. Paul Teaching Hospital from 1st of Jan 2020 to 1st of Jan 2021.ResultsOut of 1,165 annual medical admissions, the prevalence of cardiovascular diseases(CVD) was 30.3%. About 60%(212) of patients had advanced congestive heart failure of diverse causes. Hypertensive heart disease (HHD) was the next predominant diagnosis (41%(146)), and also the leading cause of cardiac diseases followed by rheumatic valvular heart disease(RVHD) (18%(64)) and Ischemic heart disease (IHD) (12.2%(43)), respectively. Yong age, rural residence and female sex were associated with RVHD(p = 0.001). Stroke also accounted for 20%(70) of CVD admission (hemorrhagic stroke-17% Vs Ischemic stroke-83%). Hypertension was the predominate risk factor for CVD and present in 46.7%(168) of patients. The mean hospital stay was 12days and in-hospital mortality rate was 24.3% with septic shock being the commonest immediate cause of death followed by fatal arrhythmia, brain herniation, and massive PTE.ConclusionCardiovascular diseases were common in the study area causing significant morbidity and mortality. Therefore, comprehensive approach is imperative to timely screen for cardiovascular risk reduction, disease control and complication prevention. Strategies should also be designed to increase public awareness regarding the cardiovascular risk reduction, drug adherence, and possible complications.
REFLEXIONES A PROPÓSITO DE LA AGENDA INTERDISCIPLINAR PENDIENTE DEL DERECHO CONSTITUCIONAL
Authors: Torres Manrique Jorge Isaac 
Published: December 2022

Contextualização: O Direito Constitucional vive inequivocamente o seu melhor momento. Isto pode ser visto não só pela multiplicidade da produção científi ca em livros e artigos, mas também pela presença profusa de eventos acadêmicos, bem como pelos importantes avanços, como o ordenamento jurídico em prol dos direitos fundamentais basilares. No entanto, o exposto não signifi ca que, necessariamente, atingiu o seu pleno desenvolvimento e sua consolidação
Objetivos: Estabelecer e analisar a agenda interdisciplinar pendente, à luz do Estado Constitucional de Direito
Metodologia: O trabalho se valeu do método indutivo para o seu desenvolvimento
Resultados: Os pontos levantados e desenvolvidos como agenda pendente difi cultam a efetiva e plena aplicação do direito constitucional, o que resulta na limitação de seus efeitos, comprometendo os ordenamentos jurídicos e os direitos e liberdades de todas as pessoas.
ÁREAS VERDES URBANAS, ESPAÇOS LIVRES PARA O LAZER
Authors: Mazzei Kátia MAZZEI , Colesanti Marlene T. Muno , Santos Douglas Gomes dos 
Published: December 2022

A criação e o manejo de Unidades de Conservação em áreas urbanas, tanto na categoria de
proteção integral como de uso sustentável, visa a melhorar a qualidade de vida do cidadão
urbano ao mesmo tempo que promove uma melhoria na qualidade ambiental urbana, dotando
o espaço urbano de cobertura vegetal e áreas verdes fundamentais Ã reprodução do ciclo
natural e manutenção do equilíbrio dinâmico. No ambiente urbano, as diferentes categorias
de espaços livres de construção (open spaces) e os índices de área verde por habitante
pretendem oferecer opções ao lazer e atividades recreativas, além de se caracterizarem como
instrumentos estratégicos para o planejamento municipal, integrando as características e os
limites do meio físico à expansão urbana.
KONSEP DARURAT NEGARA DALAM PERSPEKTIF FIQH SIYASAH DAN HUKUM TATA NEGARA STUDI KASUS TERHADAP PEMERINTAHAN PEMERINTAH DARURAT REPUBLIK INDONESIA TAHUN 1948-1949
Authors: Hilmatiar Kharismulloh 
Published: December 2022

Pembentukan PDRI berawal dari adanya Agresi Militer Belanda Kedua pada tanggal 19 Desember 1948 di Yogyakarta yang ingin menghancurkan Pemerintahan Republik Indonesia. Dalam serangan agresi tersebut, Pemerintah Pusat, terutama Presiden Soekarno dan Wakil Presiden Mohammad Hatta ditawan oleh Belanda, sehingga menyebabkan vakum dan lumpuhnya Pemerintahan. Sebelum Pemerintah Pusat ditawan oleh Belanda, Presiden telah mengumumkan pemberian mandat kepada Syafruddin Prawiranegara, untuk membentuk Pemerintahan Darurat, jika Pemerintah Pusat saat itu tidak dapat lagi meneruskan kewajibannya. Syafruddin Prawiranegara yang berada di Bukit Tinggi ketika serangan Belanda dilancarkan, tidak mengetahui adanya mandat tersebut. Hal ini disebabkan karena terputusnya jalur komunikasi antara Yogyakarta dan Bukit Tinggi akibat serangan Belanda terhadap kedua kota itu. Setelah mengetahui dengan pasti Presiden beserta pimpinan pemerintahan lainnya ditawan, bersama pemimpin sipil dan militer di Sumatera Tengah, Syafruddin Prawiranegara mendirikan PDRI tanggal 22 Desember 1948. Studi ini hendak mengkaji Pemerintahan PDRI menurut konsep darurat negara dalam perspektif fiqh siyâsah dan Hukum Tata Negara. Studi ini menunjukkan bahwa PDRI telah memainkan peranan penting dalam rangka perjuangan dan ketatanegaraan bangsa Indonesia semasa mempertahankan kemerdekaan dari Agresi Militer Belanda Kedua, khususnya dalam mempertahankan eksistensi Negara Republik Indonesia. Perjuangan PDRI tidak terlepas dari proses antara diplomasi dan perjuangan bersenjata, hal ini merupakan proses perjuangan yang dilakukan PDRI untuk mencapai Republik Indonesia yang berdaulat. Sedangkan secara hukum, baik menurut perspektif konsep siyâsah harbiyah dalam fiqh siyâsah dan Hukum Tata Negara Darurat dalam Hukum Tata Negara, Syafruddin Prawiranegara yang menjabat sebagai Ketua PDRI antara tanggal 19 Desember 1948 sampai dengan tanggal 13 Juli 1949 berhak disebut sebagai Presiden Republik Indonesia dalam keadaan darurat.
KESENJANGAN ANTARA KETENTUAN PERNIKHAN DI BAWAH UMUR DALAM FIKIH MUNAKAHAT DAN UU NO. 1 TAHUN 1974 TENTANG PERKAWINAN
Authors: Halim Abdul , Hamsin Muhammad Khaeruddin 
Published: December 2022

Sejak Pemerintah Belanda membagi rakyat Hindia Belanda menjadi tiga golongan yaitu golongan Eropa dan yang dipersamakan dengan golongan Eropa, golongan pribumi, dan golongan Timur Asing, maka sejak itu pula Hukum Perdata yang berlaku pada setiap golongan berbeda-beda. Dalam bidang perkawinan misalnya, masing-masing golongan memiliki hukum perkawinan sendiri. sehingga hukum perkawinan yang berlaku di masyarakat bersifat pluralistis. Akibatnya, kesenjangan antar system hukum tidak bisa dihindari. Undang- Undang Perkawinan lahir sebagai upaya untuk meminimalisir kesenjangan hukum tersebut, namun kenyataannya, sekalipun sudah berlaku selama kurang lebih tiga dasawarsa kesenjangan hukum di bidang perkawinan masih banyak terjadi salah satunya adalah prnikahan di bawah umur. Tulisan ini bertujuan menjelaskan mengapa masih terjadi kesenjangan antara ketentuan perkawinan di bawah umur dalam Fikih Munakahat dan UUP dan menawarkan resolusi penyelesaian dengan menggunakan teori legal system LM. Friedman, alternative kebijakan yang diintrodusir oleh Soetandyo Wignjosoebroto dan teori harmonisasi hukum yang diintrodusir oleh L.M. Gandhi.
Evaluation of the spatial variation in moisture content inside wood pieces during drying by NIR spectroscopy
Authors: Baliza Lívia Freire , Silva Carlos Henrique da , Amaral Evelize Aparecida , Ramalho Fernanda Maria Guedes , Trugilho Paulo Fernando , Sun Honggang , Hein Paulo Ricardo Gherardi 
Published: December 2022

Abstract
The moisture content (MC) of wood affects its industrial performance, but it is difficult to monitor spatial variations in MC. Here, a multivariate regression was developed to estimate the MC from near infrared (NIR) spectra and was used to monitor the spatial variation in the MC of wood during air- and oven-drying. The spectra and mass of wood pieces were measured at five stages during drying (at each 20% loss of initial water mass). Wood pieces were dried naturally and oven-dried at 60 °C. Initially, 25 spectra were recorded at equidistant points covering the entire longitudinal × radial surface of the sample. Then, a planing machine was used to access the inside of the wood, and NIR spectra were measured for each new surface, at a total of 100 points spatially distributed within the wood pieces. The wood pieces were analyzed in their original state, and when they had lost 20, 40, 60, and 80% of their initial water mass. An NIR-based regression (R
2
p = 0.90 and RMSEP = 10.51%) was applied to estimate the MC, and its spatial gradient during drying was then mapped. These analyses revealed the spatial variation in MC within wood pieces during drying.
Alginate and β-lactoglobulin matrix as wall materials for encapsulation of polyphenols to improve efficiency and stability
Authors: Sasikumar Raju , Sharma Paras , Jaiswal Amit K. 
Published: December 2022

Abstract
The present study aimed at developing novel encapsulate materials of calcium-alginate and β-lactoglobulin complex for polyphenols using the jet-flow nozzle vibration method. Encapsulated microbeads were characterized using SEM, FTIR, DSC, and MSI. The encapsulation efficiency of the microbeads varied depending upon the coating material in the range of 74.17–84.87%. Calcium-alginate-β-lactoglobulin microbeads (CABM) exhibited a smooth surface and uniform shape with an average particle size of 1053.73 nm. CABM also showed better thermal and storage stabilities as compared to calcium alginate microbeads. The CABM resulted in excellent target release of polyphenols (84%) in the intestine, which was more than 3-fold the bio-accessibility offered by free polyphenol powder. Further study on individual phenolic acids after simulated in-vitro digestion (SIVD), photo-oxidative and osmotic stress revealed that CABM significantly retained a higher amount of polyphenols and exhibited improved antioxidant capacity after SIVD environment, and may have high industrial application for nutraceutical production.
Curation as a Social Practice: Counter-Narratives in Public Space
Authors: Kathke Torsten , Tomann Juliane , Uhlig Mirko 
Published: December 2022

Abstract
The term “curation” has taken on a host of meanings beyond the museum context. While there are marked differences between its meanings – including the specific act of exhibition-making as well as the act of keeping and tending to an existing collection in a museum – we here specifically foreground curation as a social process of selecting and negotiating various forms of (embodied) performances in public, transcending institutionalized contexts such as museums. We argue that, when combined with the idea of counter-narratives, the concept of curation can elucidate aspects of social practices and open up a useful heuristic for the analysis of representations and performances in the public sphere. Since these practices make extensive use of imaginaries of the past, this approach is suitable for combining perspectives from public history and anthropology.