bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-Free Fat Extract Prevents Tail Suspension–Induced Bone Loss by Inhibiting Osteocyte Apoptosis
Authors: Xu Mingming , Du Jingke , Cui Junqi , Zhang Shuangyan , Zhang Shuhong , Deng Mingwu , Zhang Wenjie , Li Hanjun , Yu Zhifeng 
Published: January 2022

Introduction: As the space field has developed and our population ages, people engaged in space travel and those on prolonged bed rest are at increasing risk for bone loss and fractures. Disuse osteoporosis occurs frequently in these instances, for which the currently available anti-osteoporosis agents are far from satisfactory and have undesirable side effects. CEFFE is a cell-free fraction isolated from nanofat that is enriched with a variety of growth factors, and we aim to investigate its potential therapeutic effects on disuse osteoporosis.Methods: A tail suspension–induced osteoporosis model was applied in this study. Three weeks after tail suspension, CEFFE was intraperitoneally injected, and PBS was used as a control. The trabecular and cortical bone microstructures of the tibia in each group were assessed by μCT after 4 weeks of administration. Osteocyte lacunar-canalicularity was observed by HE and silver staining. In vitro, MLO-Y4 cell apoptosis was induced by reactive oxygen species (ROSUP). TUNEL staining and flow cytometry were used to detect apoptosis. CCK-8 was used to detect cell proliferation, and Western blotting was used to detect MAPK signaling pathway changes.Results: CEFFE increased the bone volume (BV/TV) and trabecular number (Tb.N) of the trabecular bone and increased the thickness of the cortical bone. HE and silver staining results showed that CEFFE reduced the number of empty lacunae and improved the lacuna-canalicular structure. CEFFE promoted osteocyte proliferative capacity in a dose-dependent manner. CEFFE protected MLO-Y4 from apoptosis by activating the serine/threonine-selective protein kinase (ERK) signaling pathways.Conclusion: CEFFE attenuated immobilization-induced bone loss by decreasing osteocyte apoptosis. CEFFE increased the survival of osteocytes and inhibited osteocyte apoptosis by activating the ERK signaling pathway in vitro.
Investigating the Influence of the Building Material on the Indoor Air Quality in Apartment in Dubai
Authors: Arar Mohammad , Jung Chuloh , Qassimi Nahla Al 
Published: January 2022

The residents of Dubai spend more than 90% of their time indoors and this lifestyle makes them easily exposed to Sick Building Syndrome (SBS). Even though Dubai Municipality strictly apply the IAQ (Indoor Air Quality) stipulation, indiscreet use of unproven finishing materials has been increased to deteriorate the health of residents in Dubai. The objective of this paper is to investigate the degree of influence of building material on indoor air pollutants concentration by measurement and prediction. As a methodology, indoor pollutants concentration was measured and investigated, variables were extracted through emission intensity experiments, and the indoor concentration was predicted by applying the double exponential decay model. The result had shown that electronic products, furniture, and textile products become new sources of indoor air pollution. The difference in emission patterns of wallpaper and flooring is confirmed via the emission rate test. It is statistically proven that Formaldehyde (CH2O) and VOCs showed a difference in the cumulative emission amount within 100 h but after that, it was confirmed that the difference in emission amount between materials became very small. In case of CH2O, the cumulative emission of the flooring material is greater than that of the wallpaper. This study will serve as a basic data to explore the cause of indoor air pollutants in daily life to reduce SBS symptoms in Dubai.
Circulating NAD+ Metabolism-Derived Genes Unveils Prognostic and Peripheral Immune Infiltration in Amyotrophic Lateral Sclerosis
Authors: Li Cheng , Zhu Yu , Chen Wenzhi , Li Menghua , Yang Mi , Shen Ziyang , Zhou Yiyi , Wang Lulu , Wang Huan , Li Shu , Ma Jiacheng , Gong Mengni , Xu Renshi 
Published: January 2022

Background: Nicotinamide adenine dinucleotide (NAD+) metabolism has drawn more attention on neurodegeneration research; however, the role in Amyotrophic Lateral Sclerosis (ALS) remains to be fully elucidated. Here, the purpose of this study was to investigate whether the circulating NAD+ metabolic-related gene signature could be identified as a reliable biomarker for ALS survival.Methods: A retrospective analysis of whole blood transcriptional profiles and clinical characteristics of 454 ALS patients was conducted in this study. A series of bioinformatics and machine-learning methods were combined to establish NAD+ metabolic-derived risk score (NPRS) to predict overall survival for ALS patients. The associations of clinical characteristic with NPRS were analyzed and compared. Receiver operating characteristic (ROC) and the calibration curve were utilized to assess the efficacy of prognostic model. Besides, the peripheral immune cell infiltration was assessed in different risk subgroups by applying the CIBERSORT algorithm.Results: Abnormal activation of the NAD+ metabolic pathway occurs in the peripheral blood of ALS patients. Four subtypes with distinct prognosis were constructed based on NAD+ metabolism-related gene expression patterns by using the consensus clustering method. A comparison of the expression profiles of genes related to NAD+ metabolism in different subtypes revealed that the synthase of NAD+ was closely associated with prognosis. Seventeen genes were selected to construct prognostic risk signature by LASSO regression. The NPRS exhibited stronger prognostic capacity compared to traditional clinic-pathological parameters. High NPRS was characterized by NAD+ metabolic exuberant with an unfavorable prognosis. The infiltration levels of several immune cells, such as CD4 naive T cells, CD8 T cells, neutrophils and macrophages, are significantly associated with NPRS. Further clinicopathological analysis revealed that NPRS is more appropriate for predicting the prognostic risk of patients with spinal onset. A prognostic nomogram exhibited more accurate survival prediction compared with other clinicopathological features.Conclusions: In conclusion, it was first proposed that the circulating NAD+ metabolism-derived gene signature is a promising biomarker to predict clinical outcomes, and ultimately facilitating the precise management of patients with ALS.
Acute Lesion Imaging in Predicting Chronic Tissue Injury in the Ventricles
Authors: El Hajjar Abdel Hadi , Huang Chao , Zhang Yichi , Mekhael Mario , Noujaim Charbel , Dagher Lilas , Nedunchezhian Saihariharan , Pottle Christopher , Kholmovski Eugene , Ayoub Tarek , Dhorepatil Aneesh , Barakat Michel , Yamaguchi Takano , Chelu Mihail , Marrouche Nassir 
Published: January 2022

BackgroundChronic lesion formation after cardiac tissue ablation is an important indicator for procedural outcome. Moreover, there is a lack of knowledge regarding the features that predict chronic lesion formation.ObjectiveThe aim of this study is to determine whether acute lesion visualization using late gadolinium enhanced magnetic resonance imaging (LGE-MRI) can reliably predict chronic lesion size.MethodsFocal lesions were created in left and right ventricles of canine models using either radiofrequency (RF) ablation or cryofocal ablation. Multiple ablation parameters were used. The first LGE-MRI was acquired within 1–5 h post-ablation and the second LGE-MRI was obtained 47–82 days later. Corview software was used to perform lesion segmentations and size calculations.Results:Fifty-Five lesions were created in different locations in the ventricles. Chronic volume size decreased by a mean of 62.5 % (95% CI 58.83–67.97, p &lt; 0.0005). Similar regression of lesion volume was observed regardless of ablation location (p = 0.32), ablation technique (p = 0.94), duration (p = 0.37), power (p = 0.55) and whether lesions were connected or not (p = 0.35). There was no significant difference in lesion volume reduction assessed at 47–54 days and 72–82 days after ablation (p = 0.31). Chronic lesion volume was equal to 0.32 of the acute lesion volumes (R2 = 0.75).ConclusionChronic tissue injury related to catheter ablation can be reliably modeled as a linear function of the acute lesion volume as assessed by LGE-MRI, regardless of the ablation parameters.
Generalizable Framework for Atrial Volume Estimation for Cardiac CT Images Using Deep Learning With Quality Control Assessment
Authors: Abdulkareem Musa , Brahier Mark S. , Zou Fengwei , Taylor Alexandra , Thomaides Athanasios , Bergquist Peter J. , Srichai Monvadi B. , Lee Aaron M. , Vargas Jose D. , Petersen Steffen E. 
Published: January 2022

ObjectivesCardiac computed tomography (CCT) is a common pre-operative imaging modality to evaluate pulmonary vein anatomy and left atrial appendage thrombus in patients undergoing catheter ablation (CA) for atrial fibrillation (AF). These images also allow for full volumetric left atrium (LA) measurement for recurrence risk stratification, as larger LA volume (LAV) is associated with higher recurrence rates. Our objective is to apply deep learning (DL) techniques to fully automate the computation of LAV and assess the quality of the computed LAV values.MethodsUsing a dataset of 85,477 CCT images from 337 patients, we proposed a framework that consists of several processes that perform a combination of tasks including the selection of images with LA from all other images using a ResNet50 classification model, the segmentation of images with LA using a UNet image segmentation model, the assessment of the quality of the image segmentation task, the estimation of LAV, and quality control (QC) assessment.ResultsOverall, the proposed LAV estimation framework achieved accuracies of 98% (precision, recall, and F1 score metrics) in the image classification task, 88.5% (mean dice score) in the image segmentation task, 82% (mean dice score) in the segmentation quality prediction task, and R2 (the coefficient of determination) value of 0.968 in the volume estimation task. It correctly identified 9 out of 10 poor LAV estimations from a total of 337 patients as poor-quality estimates.ConclusionsWe proposed a generalizable framework that consists of DL models and computational methods for LAV estimation. The framework provides an efficient and robust strategy for QC assessment of the accuracy for DL-based image segmentation and volume estimation tasks, allowing high-throughput extraction of reproducible LAV measurements to be possible.
Deciphering Degassing and Source Effects in Cl Isotopes in Melt Inclusions: The Possible Role of Amphibole in the Magma Source of Stromboli (Aeolian Island Arc)
Authors: Bouvier Anne-Sophie , Rose-Koga Estelle F. , Chapuis Alexis 
Published: January 2022

Chlorine isotopes have emerged as a new geochemical tool over the past 15 years. Most of the data consist of bulk rock data, with a minority carried out in situ on melt inclusions using secondary ion mass spectrometry. More data are necessary to understand the relationship between δ37Cl measured in melt inclusions and that in bulk rocks from the same volcanic center. Here we have analyzed a suite of melt inclusions entrapped in olivine Fo63-85, as well as some from clinopyroxene crystals, from a single hand-sample from the Vancori unit of Stromboli, Aeolian Islands. The 27 selected melt inclusions have major element compositions ranging from high potassium alkali basalt to evolved shoshonite. Their δ37Cl vary from −2.6 ± 0.1‰ to +1.2 ± 0.2‰, a far larger range than for Stromboli bulk rocks. In this dataset, the δ37Cl variation in melt inclusions is not related to Cl degassing, or to fractional crystallization. Instead, correlations between δ37Cl and S/Cl, K2O and trace element ratios suggest mixing of two Cl endmembers with distinct δ37Cl signatures. A first endmember is characterized by high potassium alkali basalt compositions, high Ba/La (∼28), high S/Cl, and high δ37Cl (&gt;1‰), confirming the influence in the mantle source of an aqueous fluid and providing a new constraint on its composition: that it derives from the breakdown of amphibole. The second endmember has a more evolved composition, high La/Yb, low S/Cl, and low δ37Cl (&lt;−2‰), identifying the influence of a solute-rich component derived from subducted sediments. The δ37Cl data thus help refine the two sources initially identified from bulk rock studies and δ37Cl proves to be a potential tracer for amphibole.
Potential Application of 4D Technology in Fabrication of Orthodontic Aligners
Authors: Elshazly Tarek M. , Keilig Ludger , Alkabani Yasmine , Ghoneima Ahmed , Abuzayda Moosa , Talaat Wael , Talaat Sameh , Bourauel Christoph P. 
Published: January 2022

Objectives: To investigate and quantify forces generated by three-dimensional-printed aligners made of shape memory polymers (four-dimensional [4D] aligner).Methods: Clear X v1.1 material was used in this study. On a custom-made typodont model, correction of maxillary central incisor (tooth 21) malposition by 4D aligners with thicknesses of 0.8 and 1.0 mm was measured by superimposition of subsequent scans. Maximum deflection forces generated by foil sheet specimens were measured at different temperatures in three-point bending (3-PB) tests. In a biomechanical system (orthodontic measurement and simulation system [OMSS]), forces generated on movements of tooth 21 by the 4D aligners were measured at different temperatures.Results: 4D aligners succeeded to achieve a significant tooth movement (2.5 ± 0.5 mm) on the typodont, with insignificant difference between different thicknesses. In the 3-PB test, the maximum deflection forces measured at 20, 30, 37, 45, and 55°C, were 3.8 ± 1.1, 2.5 ± 0.9, 1.7 ± 0.6, 1.0 ± 0.4, and 0.5 ± 0.4 N, respectively. Forces delivered on palatal displacement of tooth 21 at 37, 45, and 55°C by 0.8-mm aligners were 0.3 ± 0.1, 0.2 ± 0.1, and 0.7 ± 0.2 N, respectively, whereas those by 1.0-mm aligners were 0.3 ± 0.1, 0.3 ± 0.1, and 0.6 ± 0.2 N, respectively. A good concordance with movement on the typodont model was shown in OMSS.Conclusion: An initial study of 4D-printed aligner shows its ability to move a tooth by biocompatible orthodontic forces, after a suitable thermal stimulus within the oral temperature range.
A New Method for the Sputum Cytology Test Without Direct Contact to Specimens During COVID-19 Pandemic
Authors: Cui Junqi , Wang Xia , Rao Yamin , Ji Tianhai , Li Long 
Published: January 2022

BackgroundCoronavirus disease 2019 (COVID-19) pandemic continues to spread across the world. Specimens of blood, body fluids and excreta received in the department of pathology undoubtedly increased the risk of infection, especially in some hospitals that are short of professional protection capability. Here we provided a new simple way for the sputum cytology test during the COVID-19 pandemic.MethodsSputum samples from 30 patients with lung cancer were collected and divided into two groups, including the control group and the experimental group. Samples of the control group were processed in the biological safety cabinet, while the experimental group was put into the sealed specimen bag directly and pretreated with 75% medical alcohol. Then the cell morphology and tumor cell identification were analyzed by cell smears and liquid-based cell staining. The expression of cell antigens was determined by immunohistochemical staining.ResultOur result showed that both sputum samples in two groups exhibited complete cell structure and clear morphology according to the cell smear and liquid-based cell staining. In addition, the immunohistochemical result showed that cell antigens, including cytokeratin (CK), leukocyte common antigen (LCA), and thyroid transcription factor-1 (TTF1), were specifically expressed in the cell membrane, cytoplasm, and nucleus, respectively. The tumor cells were distributed diffusely, and cell antigens were located accurately after pretreatment with 75% medical alcohol and were consistent with that of the control group.ConclusionUsing 75% medical alcohol to pretreat sputum specimens has no obvious impact on cell morphology and antigens expression. Our study provided a new method for the sputum cytology test with no direct contact so as to protect medical staff against the virus during COVID-19 outbreak.
Differential COVID-19 Symptoms Given Pandemic Locations, Time, and Comorbidities During the Early Pandemic
Authors: Wang Yang , Zhang Fengwei , Byrd J. Brian , Yu Hong , Ye Xianwei , He Yongqun 
Published: January 2022

BackgroundCOVID-19 pandemic is disaster to public health worldwide. Better perspective on COVID's features early in its course–prior to the development of vaccines and widespread variants–may prove useful in the understanding of future pandemics. Ontology provides a standardized integrative method for knowledge modeling and computer-assisted reasoning. In this study, we systematically extracted and analyzed clinical phenotypes and comorbidities in COVID-19 patients found at different countries and regions during the early pandemic using an ontology-based bioinformatics approach, with the aim to identify new insights and hidden patterns of the COVID-19 symptoms.ResultsA total of 48 research articles reporting analysis of first-hand clinical data from over 40,000 COVID-19 patients were surveyed. The patients studied therein were diagnosed with COVID-19 before May 2020. A total of 18 commonly-occurring phenotypes in these COVID-19 patients were first identified and then classified into different hierarchical groups based on the Human Phenotype Ontology (HPO). This meta-analytic approach revealed that fever, cough, and the loss of smell and taste were ranked as the most commonly-occurring phenotype in China, the US, and Italy, respectively. We also found that the patients from Europe and the US appeared to have more frequent occurrence of many nervous and abdominal symptom phenotypes (e.g., loss of smell, loss of taste, and diarrhea) than patients from China during the early pandemic. A total of 22 comorbidities, such as diabetes and kidney failure, were found to commonly exist in COVID-19 patients and positively correlated with the severity of the disease. The knowledge learned from the study was further modeled and represented in the Coronavirus Infectious Disease Ontology (CIDO), supporting semantic queries and analysis. Furthermore, also considering the symptoms caused by new viral variants at the later stages, a spiral model hypothesis was proposed to address the changes of specific symptoms during different stages of the pandemic.ConclusionsDifferential patterns of symptoms in COVID-19 patients were found given different locations, time, and comorbidity types during the early pandemic. The ontology-based informatics provides a unique approach to systematically model, represent, and analyze COVID-19 symptoms, comorbidities, and the factors that influence the disease outcomes.
Validated Impacts of N6-Methyladenosine Methylated mRNAs on Apoptosis and Angiogenesis in Myocardial Infarction Based on MeRIP-Seq Analysis
Authors: Zhang Yingjie , Hua Wenjie , Dang Yini , Cheng Yihui , Wang Jiayue , Zhang Xiu , Teng Meiling , Wang Shenrui , Zhang Min , Kong Zihao , Lu Xiao , Zheng Yu 
Published: January 2022

Objectives: N6-methyladenosine (m6A) is hypothesized to play a role in the regulation of pathogenesis of myocardial infarction (MI). This study was designed to compare m6A-tagged transcript profiles to identify mRNA-specific changes on pathophysiological variations after MI.Methods: N6-methyladenosine methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were interacted to select m6A-modified mRNAs with samples collected from sham operated and MI rat models. m6A methylation regulated mRNAs were interacted with apoptosis/angiogenesis related genes in GeneCards. Afterwards, MeRIP-quantitative real-time PCR (MeRIP-qRT-PCR) was performed to measure m6A methylation level of hub mRNAs. m6A methylation variation was tested under different oxygen concentration or hypoxic duration in H9c2 cells and HUVECs. In addition, Western blot and qRT-PCR were employed to detect expression of hub mRNAs and relevant protein level. Flow cytometry and Tunel assay were conducted to assess apoptotic level. CCK-8, EdU, and tube formation assay were performed to measure cell proliferation and tube formation ability.Results: Upregulation of Mettl3 was firstly observed in vivo and in vitro, followed by upregulation of m6A methylation level. A total of 567 significantly changed m6A methylation peaks were identified, including 276 upregulated and 291 downregulated peaks. A total of 576 mRNAs were upregulated and 78 were downregulated. According to combined analysis of MeRIP-seq and RNA-seq, we identified 26 significantly hypermethylated and downregulated mRNAs. Based on qRT-PCR and interactive analysis, Hadh, Kcnn1, and Tet1 were preliminarily identified as hub mRNAs associated with apoptosis/angiogenesis. MeRIP-qRT-PCR assay confirmed the results from MeRIP-seq. With the inhibition of Mettl3 in H9c2 cells and HUVECs, downregulated m6A methylation level of total RNA and upregulated expression of hub mRNAs were observed. Increased m6A level was verified in the gradient context in terms of prolonged hypoxic duration and decreased oxygen concentration. Under simulated hypoxia, roles of Kcnn1 and Tet1 in angiogenesis and Hadh, Tet1, and Kcnn1 in apoptosis were further confirmed with our validation experiments.Conclusion: Roles of m6A-modified mRNA transcripts in the context of MI were preliminarily verified. In the context of m6A methylation, three hub mRNAs were validated to impact the process of apoptosis/angiogenesis. Our study provided theoretical basis and innovative targets for treatment of MI and paved the way for future investigations aiming at exploring upstream epigenetic mechanisms of pathogenesis after MI.
Mechanical Punctate Pain Thresholds in Patients With Migraine Across Different Migraine Phases: A Narrative Review
Authors: Pan Li-Ling Hope , Treede Rolf-Detlef , Wang Shuu-Jiun 
Published: January 2022

Purpose of the ReviewWe reviewed the studies of mechanical punctate pain thresholds (MPTs) in patients with migraine and summarized their findings focusing on the differences in MPT measurement and MPTs in different phases of migraine.MethodsWe searched the English-written articles that investigate the MPTs in the migraine population published in peer-reviewed journals with full-text using the PubMed, Web of Science, and Google Scholar databases. Moreover, we manually searched the references from the articles for possibly related studies.Main FindingsWe collected 276 articles and finally included twelve studies in this review. Most of the studies that included MPTs were measured with traditional von Frey filaments. The cephalic areas were always included in the assessment. Most studies compared the inter-ictal MPT in patients with migraine to controls. Among them, the majority found no significant differences; however, there were studies found either higher or lower levels of MPTs in migraine. Even though the studies provided the criteria to define the inter-ictal phase, not all of them followed up with the subjects regarding the next migraine attack. In studies that compared MPT between phases, lower MPTs were found during peri-ictal phases.SummaryChanges to MPT in migraine patients were inconclusive. The selection of measurement methods as well as properly defined migraine phases should be considered for future studies.