bluuebunny/crossref_metadata_2025_split huggingface dataset:
Praxeological judgment of conceptualization and theoretical models of the memory phenomenon in the study of J. Baudrillard's heritage
Authors: Maltsev O. 
Published: June 2022

The nature of global world transformations correlated to overwhelming systemic crises and contradictions and the transition of humanity to a new course of civilizational development, which is characterized by dynamic processes of digitization, the spread of hyperreality, the transition of human activity towards a virtual dimension. Current trends determine the prerequisites of ontological and epistemological understanding of sustainable social development vector, as well as relevant strategies for the educational improvement aimed at gaining relevant skills that allow one to achieve his goals and results.
The goal of the following scientific exploration is to apply the heuristic memory models knowledge to the interpretation of the praxiological results; it is directed at analyzing the heritage of Jean Baudrillard's personality, his projects, contributions to the development of philosophy and science; issues preserved in the living memory of experts for methodological value in education and self-education, in the practice of developing the skills, as well as relevant skills regarding the next generations. This praxeological concept is aimed at verifying the results of philosophical interpretation of theoretical models of the memory phenomenon in the practice of understanding the philosophy of J. Baudrillard and his memory heritage.
Within the activity approach we have comprehended works of academician G. Popov, in the tradition of his school and in solidarity with his concepts of activity approach to the study of generations’ memory, thus, we analyse practical lessons in teaching and educating relevant skills in the individual, verified by the requirements of society, such a philosophical under-standing contributed to the understanding of ontological, epistemological, axiological and praxeological aspects of further analysis. Based on the principle of unity of theory and practice, we managed to develop a system of practice-philosophical analysis in the application of semi-structured interviews experts, who are followers, successors, critics and independent researchers, i.e., the «Carrier data» of the philosophical, anthropological, psychological and sociological memory inheritance of Jean Baudrillard, the ‘productive’ figure in history, who for a reason is also the subject claimed to be the "Europe's last prophet».
Concerning the results of the scientific exploration, we shall consider the role and influence of the environment and world memory "constructs" and history on the memory formation of an authentic person, his works, achievements and results of creativity, on the determination of memory content of an individual, as well as on wherewith these data may be used in contemporary conditions from an active approach to the philosophical and practical conclusion of methodologies and educational technologies relevant and promising in the 21st century.
Language of web: bynet discourse and Belarusian netspeak
Authors: Mojeiko Marina A. 
Published: June 2022

It is proved that the modern Internet space creates a fundamentally new communication environment (social networks, chats, etc.), the capabilities of which, in turn, act as a trigger for linguistic transformations. It is the language of Internet communications that is today the field where new trends in language development are most actively formed. The functioning of the language in the network gives rise to a number of new phenomena. So, Internet discourse gives rise to such phenomena as massive abbreviations and an abundance of neologisms (including game ones), acronyms and backronyms, syngrahemics and supragrahemics, interlanguage hybrids, new tools for expressing subject-subject relations (formulas of politeness, for example) and many others. This poses new problems for both linguistics and philosophy of language. For example, it is shown that in the context of Internet communications, the classic dichotomy of verbal speech and written forms of language is blurred: on the one hand, in chats and forums, the language is formally represented by written texts, on the other – interactive dialogues actually set living one-time communication, which allows one to speak simultaneously, interrupt the interlocutor, respond immediately, etc., and all these are characteristic of verbal speech. It is concluded that if until now the main presenter of language in culture has clearly been fiction as embodying the established norm of the correct language, then today language practices (usus) come to the fore, reflecting the trends of linguistic evolution that are forming in the present continuous, that sets a new accentuation in modern culture, namely the prevalence of descriptive linguistics over prescriptive.
Diet Analysis of Sympatric Colobine Monkeys from Bako National Park, Sarawak, Borneo
Authors: WAN AZMAN WAN NUR SYAFINAZ , KHAN FAISAL ALI ANWARALI 
Published: June 2022

Habitat quality and abundant of food resources are among the key factors influencing the continued existence of primates in the wild. Although much has been studied on primate habitats and their diets, little is known about the nutritional value of the colobines’ foods. This study aimed to assess the dietary nutrient compositions of two sympatric colobine monkeys, Trachypithecus cristatus and Nasalis larvatus, in Bako National Park using proximate analysis of faecal, leaf and fruit samples of eight dominant tree species in Bako NP. Five nutrient parameters, namely crude protein, crude fat, crude fibre, ash, phosphorus, and energy content, were choosen to assess the nutritional demands of the monkeys in the wild. The faecal samples showed significantly higher percentage of crude fibre (27.58%) in N. larvatus compared to T. cristatus. In contrast, crude fat (8.52%), ash content (1.79%) and phosphorus (5.76 mg/g) were found to be significantly higher in the faecal samples of T. cristatus than in N. larvatus. The nutrient composition of leaves samples from the tree species consumed by N. larvatus and T. cristatus showed a significantly higher percentage of crude protein (14.56%) in Barringtonia asiatica (sea poison tree) and higher ash (13.70%) in Morinda citrifolia (Indian mulberry). Meanwhile, nutrient composition in fruit samples showed highest percentage of crude fibre (32.58%) and crude fat (12.35%) in Calophyllum inophyllum (Alexandrian laurel), whereas higher phosphorus (5.76%) and energy (24.26 KJ) were recorded in Ceriops tagal (Yellow mangrove). The higher crude fiber detected in N. larvatus’ faecal samples compared to T. cristatus may indicates that N. larvatus experiences lower digestibility as they are incapable of completely digesting the tough leaves or fruits. This study provides useful information for the conservation and management of these primate species especially on their dietary requirements in captivity or in a new habitat.
Sanitasi Terminal pada Era New Normal : Studi Kasus Terminal Tipe A Simbuang Mamuju
Authors: Islam Fahrul , Erwin Ashari Agus , Ahmad Haeranah 
Published: June 2022

Poor access to sanitation is one of the causes of disease in public places including at bus station. This research uses observational methods with a descriptive approach. The purpose of this study was to determine the sanitary condition of Simbuang Mamuju Type A Station. The object of this study is The Environmental Sanitation Facility of the Simbuang Type A Station, Mamuju Regency. The instrument used is an assessment instrument for environmental health examination of public transportation infrastructure by the Health Human Resources Education Center in 2018. The results showed that the condition of the clean water supply facilities was qualified with a percentage of the total score of 70%, but there were still components that did not meet the requirements, namely the components of periodic clean water quality inspection in the lab. Toilet conditions are not eligible with a percentage of the total score below 70%, which is 65%. unqualified components are toilets that are not clean and smelly. The condition of the waste disposal facility is qualified with a percentage of the total score of 87.5%, but there are still components that do not meet the requirements, namely the waste component is disposed of / transported > every 3 days. Conclusion: The Sanitation Condition of the Simbuang Type A Station is not healthy yet. Suggestion: all components that are not qualified to be paid more attention to.
Engineering Glucose-to-Glycerol Pathway in Klebsiella pneumoniae and Boosting 3-Hydroxypropionic Acid Production Through CRISPR Interference
Authors: Liu Hexin , Zhao Peng , Tian Pingfang 
Published: June 2022

The recent decline of the international biodiesel industry has led to decreased production and therefore increased the price of glycerol, which is a major by-product of biodiesel but a substrate for production of 3-hydroxypropionic acid (3-HP), that is, glycerol as a feedstock has no advantage over glucose in price. Hence, we engineered glucose to the glycerol pathway and improved 3-HP production by CRISPR interference (CRISPRi). To begin with, we cloned the genes encoding glycerol 3-phosphate dehydrogenase (gpd1) and glycerol 3-phosphatase (gpp2) from Saccharomyces cerevisiae, which jointly catalyze glucose into glycerol. The genes gpd1 and gpp2 were co-expressed in K. pneumoniae with the dCas9 gene integrated in genome, and this recombinant strain produced 2 g/L glycerol in the shake flask. To minimize the glucose consumption by competing pathways including the EMP pathway, glycerol oxidation pathway, and by-products pathways, we developed an CRISPRi system in aforementioned recombinant K. pneumoniae strain to inhibit the expression of the glyceraldehyde-3-phosphate dehydrogenase gene (gapA) and 2,3-butanediol production gene (budA), resulting in a bi-functional strain harboring both glucose-to-glycerol pathway and CRISPRi system. Reverse transcription and quantitative PCR (RT-qPCR) results showed that this engineered CRISPRi system transcriptionally inhibited gapA and budA by 82% and 24%, respectively. In shake flask cultivation, this bi-functional strain produced 2.8 g/L glycerol using glucose as the carbon source, which was 46.6% increase compared to the strain without the engineered CRISPRi system. Moreover, this bi-functional strain produced 0.78 g/L 3-HP using glucose as the sole carbon source. In fed-batch cultivation, this bi-functional strain produced 1.77 g/L 3-HP. This study provides insights for co-utilization of distinct carbon sources.
Asexual Blood-Stage Malaria Vaccine Candidate PfRipr5: Enhanced Production in Insect Cells
Authors: Correia Ricardo , Fernandes Bárbara , Castro Rute , Nagaoka Hikaru , Takashima Eizo , Tsuboi Takafumi , Fukushima Akihisa , Viebig Nicola K. , Depraetere Hilde , Alves Paula M. , Roldão António 
Published: June 2022

The malaria asexual blood-stage antigen PfRipr and its most immunogenic fragment PfRipr5 have recently risen as promising vaccine candidates against this infectious disease. Continued development of high-yielding, scalable production platforms is essential to advance the malaria vaccine research. Insect cells have supplied the production of numerous vaccine antigens in a fast and cost-effective manner; improving this platform further could prove key to its wider use. In this study, insect (Sf9 and High Five) and human (HEK293) cell hosts as well as process-optimizing strategies (new baculovirus construct designs and a culture temperature shift to hypothermic conditions) were employed to improve the production of the malaria asexual blood-stage vaccine candidate PfRipr5. Protein expression was maximized using High Five cells at CCI of 2 × 106 cell/mL and MOI of 0.1 pfu/cell (production yield = 0.49 mg/ml), with high-purity PfRipr5 binding to a conformational anti-PfRipr monoclonal antibody known to hold GIA activity and parasite PfRipr staining capacity. Further improvements in the PfRipr5 expression were achieved by designing novel expression vector sequences and performing a culture temperature shift to hypothermic culture conditions. Addition of one alanine (A) amino acid residue adjacent to the signal peptide cleavage site and a glycine-serine linker (GGSGG) between the PfRipr5 sequence and the purification tag (His6) induced a 2.2-fold increase in the expression of secreted PfRipr5 over using the expression vector with none of these additions. Performing a culture temperature shift from the standard 27–22°C at the time of infection improved the PfRipr5 expression by up to 1.7 fold. Notably, a synergistic effect was attained when combining both strategies, enabling to increase production yield post-purification by 5.2 fold, with similar protein quality (i.e., purity and binding to anti-PfRipr monoclonal antibody). This work highlights the potential of insect cells to produce the PfRipr5 malaria vaccine candidate and the importance of optimizing the expression vector and culture conditions to boost the expression of secreted proteins.
Sustainable Production of Bioethanol Using Levulinic Acid Pretreated Sawdust
Authors: Nawaz Ali , Huang Rong , Junaid Farah , Feng Yiwei , Haq Ikram Ul , Mukhtar Hamid , Jiang Kankan 
Published: June 2022

The sustainability and economic viability of the bioethanol production process from lignocellulosic biomass depend on efficient and effective pretreatment of biomass. Traditional pretreatment strategies implicating the use of mineral acids, alkalis, and organic solvents release toxic effluents and the formation of inhibitory compounds posing detrimental effects on the environment and interfering with the enzymatic saccharification process, respectively. Ionic liquids (ILs) as green solvents were used to overcome this issue, but the deep eutectic solvent as an emerging class of ionic liquids performed better in terms of making the process environmentally and economically viable. The green solvent-based pretreatment strategy applied in the current research was levulinic, acid-based natural deep eutectic solvent (NADES). Three different hydrogen bond acceptors (HBAs)—acetamide, betaine, and choline chloride—in combination with levulinic acid as hydrogen bond donor (HBD) in (HBD: HBA) molar ratio 2:1, were screened for biomass pretreatment. The best deep eutectic solvent was levulinic acid: choline chloride in an optimized molar ratio of 1:0.5, resulting in 91% delignification. The physicochemical parametric optimization of saccharification exhibited maximum enzymatic hydrolysis of 25.87% with 125 mg of pretreated sawdust via simultaneous addition of three thermostable cellulases [i.e., endo-1,4-β-D-glucanase (240 U), exo-1,4-β-D-glucanase (180 U), and β-glucosidase (320 U)] for 5 h of incubation at 75°C. The reducing sugar slurry obtained from the saccharified biomass was then added to a fermentation medium for bioethanol production, and a maximum of 11.82% of production was obtained at 30°C, 72 h, and 180 rpm using a 2.5% 24 h old Saccharomyces cerevisiae seed culture. The current study revealed that the levulinic-based deep eutectic solvent exhibited remarkable delignification, which led to the efficient enzymatic hydrolysis of sawdust and hence bioethanol production. Furthermore, it will prospect new avenues in bioethanol production using a deep eutectic solvent. Deep eutectic solvent overcame the issues posed by ionic liquids: toxicity, expensive and complex preparation, and non-biodegradability.
Laboratory Screening of Control Agents Against Isolated Fungal Pathogens Causing Postharvest Diseases of Pitaya in Guizhou, China
Authors: Li Yong , Chen Haijiang , Ma Lan , An Youshan , Wang Hui , Wu Wenneng 
Published: June 2022

Pitaya, or dragon fruit, is a typical tropical fruit with an appealing taste and diverse health benefits to humans. The plantation of pitaya in Guizhou province in China has greatly boosted the income of local farmers and alleviated poverty. However, the frequent occurrence of postharvest diseases has brought large economic loss. To find a solution, we set out to identify the postharvest disease-causing agents of Guizhou pitaya. Several fungi were isolated from diseased pitaya and identified as species based on the ITS1 sequence similarity. Of them, Penicillium spinulosum, Phoma herbarum, Nemania bipapillata, and Aspergillus oryzae were, for the first time, found to cause dragon fruit disease. In consideration of their prevalence in postharvest fruit diseases, Alternaria alternata H8 and Fusarium proliferatum H4 were chosen as representative pathogens for the drug susceptibility test. Among the tested drugs and plant extracts, 430 g/L tebuconazole and 45% prochloraz were found to be the most potent fungicides against H8 and H4, respectively. The research provides insights into the mechanism and control of postharvest diseases of dragon fruits in Guizhou, China, and thus could be of economic and social significance to local farmers and the government.
Fabrication of Ag/ZnO@N-Carbon Core@Shell Photocatalyst for Efficient Photocatalytic Degradation of Rhodamine B
Authors: Yang Xiaobing , Hu Jiapeng , Pan Junjie , Shen Yongbin , Cheng Kejun 
Published: June 2022

Photocatalytic degradation method has been recognized as an effective way to eliminate the contamination of environment. However, developing photocatalysts with excellent photocatalytic properties are still a big challenge. In this paper, Ag doped ZnO coating with a layer of N doped porous carbon (Ag/ZnO@N-carbon) was successfully synthesized by using polyvinyl pyrrolidone (PVP) modified ZIF-8 as precursor via adsorption, hydrothermal treatment, in situ growth and carbonization processes. The physical and chemical properties of all samples were characterized by X-ray powder diffraction (XRD), scanning electron microscope (SEM), electron transmission microscopy (TEM) and so on. The results show that Ag doping does not change the crystallinity of ZnO, but broaden its photo-response property. The coating of N doped carbon can improve the specific surface area of photocatalyst. The photocatalytic activity of all samples was evaluated by degradation of rhodamine B (RhB) solution under UV light irradiation for 25 min. Ag/ZnO@N-carbon exhibits the highest photocatalytic activity for degradation of RhB with a degradation of 98.65%. Furthermore, Ag/ZnO@N-carbon also has high stability. Based on the characterization, possible mechanism for degradation of RhB by Ag/ZnO@N-carbon under UV light irradiation was proposed.
Sex Differences in Intracranial Atherosclerotic Plaques Among Patients With Ischemic Stroke
Authors: Yan Xuejiao , Tang Min , Gao Jie , Wang Lihui , Li Ling , Ma Niane , Shi Xiaorui , Lei Xiaoyan , Zhang Xiaoling 
Published: June 2022

ObjectiveHigh-risk intracranial arterial plaques are the most common cause of ischemic stroke and their characteristics vary between male and female patients. However, sex differences in intracranial plaques among symptomatic patients have rarely been discussed. This study aimed to evaluate sex differences in intracranial atherosclerotic plaques among Chinese patients with cerebral ischemia.MethodsOne hundred and ten patients who experienced ischemic events underwent 3T cardiovascular magnetic resonance vessel wall scanning for the evaluation of intracranial atherosclerotic disease. Each plaque was classified according to its likelihood of causing a stroke (as culprit, uncertain, or non-culprit). The outer wall area (OWA) and lumen area of the lesion and reference sites were measured, and the wall and plaque areas, remodeling ratio, and plaque burden (characterized by a normalized wall index) were further calculated. The composition (T1 hyperintensity, enhancement) and morphology (surface irregularity) of each plaque were analyzed. Sex differences in intracranial plaque characteristics were compared between male and female patient groups.ResultsOverall, 311 plaques were detected in 110 patients with ischemic stroke (81 and 29 male and female patients, respectively). The OWA (P &lt; 0.001) and wall area (P &lt; 0.001) of intracranial arterial lesions were significantly larger in male patients. Regarding culprit plaques, the plaque burden in male patients was similar to that in female patients (P = 0.178, odds ratio [OR]: 0.168, 95% confidence interval [CI]: −0.020 to 0.107). However, the prevalence of plaque T1 hyperintensity was significantly higher than that in female patients (P = 0.005, OR: 15.362, 95% CI: 2.280–103.49). In the overall ischemic stroke sample, intracranial T1 hyperintensity was associated with male sex (OR: 13.480, 95% CI: 2.444–74.354, P = 0.003), systolic blood pressure (OR: 1.019, 95% CI: 1.002–1.036, P = 0.031), and current smoker (OR: 3.245, 95% CI: 1.097–9.598, P = 0.033).ConclusionFor patients with ischemic stroke, the intracranial plaque burden in male patients was similar to that in female patients; however, the plaque characteristics in male patients are associated with higher risk, especially in culprit plaques.
Rationale and Design of the ISOLATION Study: A Multicenter Prospective Cohort Study Identifying Predictors for Successful Atrial Fibrillation Ablation in an Integrated Clinical Care and Research Pathway
Authors: Verhaert Dominique V. M. , Linz Dominik , Chaldoupi Sevasti Maria , Westra Sjoerd W. , den Uijl Dennis W. , Philippens Suzanne , Kerperien Mijke , Habibi Zarina , Vorstermans Bianca , ter Bekke Rachel M. A. , Beukema Rypko J. , Evertz Reinder , Hemels Martin E. W. , Luermans Justin G. L. M. , Manusama Randolph , Lankveld Theo A. R. , van der Heijden Claudia A. J. , Bidar Elham , Hermans Ben J. M. , Zeemering Stef , Bijvoet Geertruida P. , Habets Jesse , Holtackers Robert J. , Mihl Casper , Nijveldt Robin , van Empel Vanessa P. M. , Knackstedt Christian , Simons Sami O. , Buhre Wolfgang F. F. A. , Tijssen Jan G. P. , Isaacs Aaron , Crijns Harry J. G. M. , Maesen Bart , Vernooy Kevin , Schotten Ulrich 
Published: June 2022

IntroductionContinuous progress in atrial fibrillation (AF) ablation techniques has led to an increasing number of procedures with improved outcome. However, about 30–50% of patients still experience recurrences within 1 year after their ablation. Comprehensive translational research approaches integrated in clinical care pathways may improve our understanding of the complex pathophysiology of AF and improve patient selection for AF ablation.ObjectivesWithin the “IntenSive mOlecular and eLectropathological chAracterization of patienTs undergoIng atrial fibrillatiOn ablatioN” (ISOLATION) study, we aim to identify predictors of successful AF ablation in the following domains: (1) clinical factors, (2) AF patterns, (3) anatomical characteristics, (4) electrophysiological characteristics, (5) circulating biomarkers, and (6) genetic background. Herein, the design of the ISOLATION study and the integration of all study procedures into a standardized pathway for patients undergoing AF ablation are described.MethodsISOLATION (NCT04342312) is a two-center prospective cohort study including 650 patients undergoing AF ablation. Clinical characteristics and routine clinical test results will be collected, as well as results from the following additional diagnostics: determination of body composition, pre-procedural rhythm monitoring, extended surface electrocardiogram, biomarker testing, genetic analysis, and questionnaires. A multimodality model including a combination of established predictors and novel techniques will be developed to predict ablation success.DiscussionIn this study, several domains will be examined to identify predictors of successful AF ablation. The results may be used to improve patient selection for invasive AF management and to tailor treatment decisions to individual patients.