bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histidine–tryptophan–ketoglutarate solution versus multidose cardioplegia for myocardial protection in cardiac surgeries: a systematic review and meta-analysis
Authors: Albadrani Muayad 
Published: May 2022

Abstract
Background
Surgical procedures in the heart requires protection of the heart from ischemia–reperfusion injury. Cardioplegia is the primary myocardial protective method in use. Histidine–tryptophan–ketoglutarate (HTK) solution is an intracellular cardioplegic solution that was initially used to preserve organs for transplantation.

Methods
A systematic electronic search was conducted in July 2021, in four databases; PubMed, Scopus, Web of Science, and Cochrane Library for eligible randomized controlled trials. The results were screened and the eligible trials were identified. Thereafter, the relevant data were extracted and pooled as mean difference or risk ratio, and 95% confidence interval in an inverse variance method using RevMan software.

Results
This review included 12 trials (n = 1327). HTK solution has resulted significantly in shorter intensive care unit stay (MD = − 0.09; 95% CI [− 0.15, − 0.03], p = 0.006), and shorter hospital stay (MD = − 0.51; 95% CI [− 0.71, − 0.31], p < 0.00001). Moreover, the patients who received the HTK solution had significantly lower levels of creatine kinase (after 4–7 h (MD = − 157.52; 95% CI [− 272.31, − 42.19], p = 0.007), and 24 h (MD = − 136.62; 95% CI [− 267.20, − 6.05], p = 0.04)), as well as creatine kinase muscle brain band (after 44–48 h (MD = − 3.35; 95% CI [− 5.69, − 1.02], p = 0.005)).

Conclusion
HTK solution had the same efficacy and safety as other cardioplegic solutions in most of the clinical parameters. Furthermore, the solution showed superiority in fastening the recovery and protecting the myocardium at the biochemical level. HTK solution provides longer myocardial protection; therefore, it limits surgical interruption. HTK solution can be used as an alternative to the currently used cardioplegic solutions.
High-risk twin pregnancy: case report of an adolescent patient with cystic fibrosis and systemic lupus erythematosus
Authors: Sanseverino Paula Baptista , Hoffmann Anneliese , Machado Sandra , Farias Mariana , Michels Marcus Silva , Sanseverino Maria Teresa Vieira , Marostica Paulo José Cauduro 
Published: May 2022

Abstract
Background
We present the first case to our knowledge of a spontaneous twin pregnancy in a 16-year-old Caucasian patient with cystic fibrosis and systemic lupus erythematosus. Cystic fibrosis is one of the most common autosomal recessive genetic disorders and primarily affects the respiratory and digestive systems. Systemic lupus erythematosus is a chronic inflammatory disease of unknown cause that affects nearly every organ. Patients with cystic fibrosis or systemic lupus erythematosus are progressively having longer life expectancy and better quality of life, which has led a greater number of female patients reporting the desire to become mothers.

Case presentation
We present a case of a Caucasian 16-year-old pregnant with twins being treated for both cystic fibrosis and systemic lupus erythematosus. She has two CFTR mutations: p.F508del and 1812_1G>A. In the second trimester, she was admitted for possible preterm labor, which was successfully stopped. The patient’s nutritional status worsened, and she had a pulmonary exacerbation as well as a flare of systemic lupus erythematosus. At the 28th gestational week, she presented with a massive hemoptysis episode. The cesarean delivery had no complications, and there were no serious immediate postpartum complications.

Discussion and conclusions
While adolescent pregnancies in and of themselves are considered high risk for both the young mothers and their children, they are further complicated when the mother has two chronic diseases and a twin pregnancy. We achieved positive results using a multidisciplinary approach; however, the risks involved were so high that major efforts are to be taken by our medical community to prevent unplanned pregnancies in all patients with cystic fibrosis, especially when a serious comorbidity like the one in this case is present.
Mxc, a Drosophila homolog of mental retardation-associated gene NPAT, maintains neural stem cell fate
Authors: Sang Rong , Wu Cheng , Xie Shanshan , Xu Xiao , Lou Yuhan , Ge Wanzhong , Xi Yongmei , Yang Xiaohang 
Published: May 2022

Abstract
Background
Mental retardation is a complex neurodevelopmental disorder. NPAT, a component of the histone locus body (HLB), has been implicated as a candidate gene for mental retardation, with a mechanism yet to be elucidated.

Results
We identified that mxc, the Drosophila ortholog of NPAT, is required for the development of nervous system. Knockdown of mxc resulted in a massive loss of neurons and locomotion dysfunction in adult flies. In the mxc mutant or RNAi knockdown larval brains, the neuroblast (NB, also known as neural stem cell) cell fate is prematurely terminated and its proliferation potential is impeded concurrent with the blocking of the differentiation process of ganglion mother cells (GMCs). A reduction of transcription levels of histone genes was shown in mxc knockdown larval brains, accompanied by DNA double-strand breaks (DSBs). The subsidence of histone transcription levels leads to prematurely termination of NB cell fate and blockage of the GMC differentiation process. Our data also show that the increase in autophagy induced by mxc knockdown in NBs could be a defense mechanism in response to abnormal HLB assembly and premature termination of NB cell fate.

Conclusions
Our study demonstrate that Mxc plays a critical role in maintaining neural stem cell fate and GMC differentiation in the Drosophila larval brain. This discovery may shed light on the understanding of the pathogenesis of NPAT-related mental retardation in humans.
Claudin18.2 is a novel molecular biomarker for tumor-targeted immunotherapy
Authors: Cao Weijie , Xing Haizhou , Li Yingmei , Tian Wenliang , Song Yongping , Jiang Zhongxing , Yu Jifeng 
Published: May 2022

AbstractThe claudin18.2 (CLDN18.2) protein, an isoform of claudin18, a member of the tight junction protein family, is a highly selective biomarker with limited expression in normal tissues and often abnormal expression during the occurrence and development of various primary malignant tumors, such as gastric cancer/gastroesophageal junction (GC/GEJ) cancer, breast cancer, colon cancer, liver cancer, head and neck cancer, bronchial cancer and non-small-cell lung cancer. CLDN18.2 participates in the proliferation, differentiation and migration of tumor cells. Recent studies have identified CLDN18.2 expression as a potential specific marker for the diagnosis and treatment of these tumors. With its specific expression pattern, CLDN18.2 has become a unique molecule for targeted therapy in different cancers, especially in GC; for example, agents such as zolbetuximab (claudiximab, IMAB362), a monoclonal antibody (mAb) against CLDN18.2, have been developed. In this review, we outline recent advances in the development of immunotherapy strategies targeting CLDN18.2, including monoclonal antibodies (mAbs), bispecific antibodies (BsAbs), chimeric antigen receptor T (CAR-T) cells redirected to target CLDN18.2, and antibody–drug conjugates (ADCs).
The polyG diseases: a new disease entity
Authors: Liufu Tongling , Zheng Yilei , Yu Jiaxi , Yuan Yun , Wang Zhaoxia , Deng Jianwen , Hong Daojun 
Published: May 2022

AbstractRecently, inspired by the similar clinical and pathological features shared with fragile X-associated tremor/ataxia syndrome (FXTAS), abnormal expansion of CGG repeats in the 5’ untranslated region has been found in neuronal intranuclear inclusion disease (NIID), oculopharyngeal myopathy with leukoencephalopathy (OPML), and oculopharyngodistal myopathy (OPDMs). Although the upstream open reading frame has not been elucidated in OPML and OPDMs, polyglycine (polyG) translated by expanded CGG repeats is reported to be as a primary pathogenesis in FXTAS and NIID. Collectively, these findings indicate a new disease entity, the polyG diseases. In this review, we state the common clinical manifestations, pathological features, mechanisms, and potential therapies in these diseases, and provide preliminary opinions about future research in polyG diseases.
The dural angioleiomyoma harbors frequent GJA4 mutation and a distinct DNA methylation profile
Authors: Tauziède-Espariat Arnault , Pierre Thibaut , Wassef Michel , Castel David , Riant Florence , Grill Jacques , Roux Alexandre , Pallud Johan , Dezamis Edouard , Bresson Damien , Benichi Sandro , Blauwblomme Thomas , Benzohra Djallel , Gauchotte Guillaume , Pouget Celso , Colnat-Coulbois Sophie , Mokhtari Karima , Balleyguier Corinne , Larousserie Frédérique , Dangouloff-Ros Volodia , Boddaert Nathalie , Debily Marie-Anne , Hasty Lauren , Polivka Marc , Adle-Biassette Homa , Métais Alice , Lechapt Emmanuèle , Chrétien Fabrice , Sahm Felix , Sievers Philipp , Varlet Pascale ,   the RENOCLIP-LOC
Published: May 2022

AbstractThe International Society for the Study of Vascular Anomalies (ISSVA) has defined four vascular lesions in the central nervous system (CNS): arteriovenous malformations, cavernous angiomas (also known as cerebral cavernous malformations), venous malformations, and telangiectasias. From a retrospective central radiological and histopathological review of 202 CNS vascular lesions, we identified three cases of unclassified vascular lesions. Interestingly, they shared the same radiological and histopathological features evoking the cavernous subtype of angioleiomyomas described in the soft tissue. We grouped them together with four additional similar cases from our clinicopathological network and performed combined molecular analyses. In addition, cases were compared with a cohort of 5 soft tissue angioleiomyomas. Three out 6 CNS lesions presented the same p.Gly41CysGJA4mutation recently reported in hepatic hemangiomas and cutaneous venous malformations and found in 4/5 soft tissue angioleiomyomas of our cohort with available data. Most DNA methylation profiles were not classifiable using the CNS brain tumor (version 12.5), and sarcoma (version 12.2) classifiers. However, using unsupervised t-SNE analysis and hierarchical clustering analysis, 5 of the 6 lesions grouped together and formed a distinct epigenetic group, separated from the clusters of soft tissue angioleiomyomas, other vascular tumors, inflammatory myofibroblastic tumors and meningiomas. Our extensive literature review identified several cases similar to these lesions, with a wide variety of denominations. Based on radiological and histomolecular findings, we suggest the new terminology of “dural angioleiomyomas” (DALM) to designate these lesions characterized by a distinct DNA methylation pattern and frequentGJA4mutations.
Social media analysis of car parking behavior using similarity based clustering
Authors: Arhab Nabil , Oussalah Mourad , Jahan Md Saroar 
Published: May 2022

AbstractThis paper investigates car parking users’ behaviors from social media perspective using social network based analysis of online communities revealed by mining the associated hashtags in Twitter. We propose a newinterpretablecommunity detection approach for mapping user’s car parking behavior by combining Clique, K-core and Girvan–Newman community detection algorithms together with a content-based analysis that exploits polarity, relative frequency and dominant topics. Twitter API was used to collect relevant data by tracking popular car-parking hashtags. A social network graph is constructed using a similarity-based analysis. Finally, interpretable communities are inferred by monitoring the outcomes of clique, K-core and Girvan–Newman community detection algorithms. This interpretability is linked to the aggregation of keywords, hashtags and/or location attributes of the tweet messages as well as a visualization module that enables interaction with users. In parallel, a global trend analysis investigates parking types and Twitter influence with respect to both sentiment polarity and dominant trends (extracted using KeyBERT based approach) is performed. The implementation of this social media analytics has uncovered several aspects associated to car-parking behaviors. A comparison with some state-of-the-art community detection methods has also been carried out and revealed some similarities with our developed approach.
Earthquake probability in the National Seismic Hazard Maps for Japan and people’s risk perceptions: the search for more effective expression
Authors: Saito Sayaka , Yasumoto Shinya , Sekiya Naoya 
Published: May 2022

AbstractThis study summarizes the findings of an attitude survey focused on people’s perceptions of seismic hazard maps, which illustrate the risk of an earthquake in each location throughout Japan. These seismic hazard maps depict the likelihood of an earthquake with a seismic intensity of “6 Lower” or more within 30 years, with 3.0% being considered a high possibility. The 3.0% occurrence probability within 30 years can be reworded to 2.0% occurrence probability within 20 years, 0.1% occurrence probability within a year, 4.9% occurrence probability within 50 years, or 39.4% occurrence probability within 500 years when being converted according to a Poisson distribution. In this study, we convert the occurrence probability within 30 years in the seismic hazard maps to obtain that within a year up to that within 1000 years, present the involved risk and demonstrate the yearly probability of the people who see the maps becoming most convinced of the need for countermeasures. Although those are shorter than the occurrence probability within 30 years anyway, it is a characteristic that the peaks existed at the occurrence probability within 10–20 years, not at the occurrence probability within one year or five years. However, the results changed according to the age of the people viewing the maps, and it was when the risk was presented as the occurrence probabilities within 20 and 30 years for the people in their 20–40 s, while it was when the risk was presented the occurrence probabilities within 10 years for the people in their 50–60 s, the perception toward the need of countermeasures increased the most. In addition, regardless of gender and place of residence (coast of the Sea of Japan or the Pacific coast), the perception toward the need of countermeasures peaked at the occurrence probabilities within 10 and 20 years.
Graphical Abstract
Improved succinic acid production through the reconstruction of methanol dissimilation in Escherichia coli
Authors: Guo Feng , Wu Min , Zhang Shangjie , Feng Yifan , Jiang Yujia , Jiang Wankui , Xin Fengxue , Zhang Wenming , Jiang Min 
Published: May 2022

AbstractSynthetic biology has boosted the rapid development on using non-methylotrophy as chassis for value added chemicals production from one-carbon feedstocks, such as methanol and formic acid. The one-carbon dissimilation pathway can provide more NADH than monosaccharides including glucose, which is conducive for reductive chemicals production, such as succinic acid. In this study, the one-carbon dissimilation pathway was introduced in E. coli Suc260 to enhance the succinic acid production capability. Through the rational construction of methanol dissimilation pathway, the succinic acid yield was increased from 0.91 to 0.95 g/g with methanol and sodium formate as auxiliary substrates in anaerobic fed-batch fermentation. Furthermore, the metabolic flux of by-product pyruvate was redirected to succinic acid together with the CO2 fixation. Finally, through the immobilization on a specially designed glycosylated membrane, E. coli cells are more resistant to adverse environments, and the final yield of succinic acid was improved to 0.98 g/g. This study proved the feasibility of endowing producers with methanol dissimilation pathway to enhance the production of reductive metabolites.
Graphical Abstract
Review of Fire ecology and management: past, present, and future of US forested ecosystems by Cathryn H. Greenberg and Beverly Collins (editors) and 75 contributing authors
Authors: Chaideftou Evgenia 
Published: May 2022

Abstract
Background
Fire is a multifaceted force. Fire activity and risk of fire incidence across US forested ecosystems have accelerated over the last two decades. At the same time, human land-use choices and climate change interacted with fire, in an era we are called to meet specific sustainability goals of reducing CO2 emissions and protecting biodiversity, ecosystems, and their resources. To understand the development of the traits and functions of organisms, and the processes and drivers of natural and human-induced events that cause fire, it is essential to study the fire ecology in the context of human land-use history in the forested ecoregions of the USA.

Results
This book is a research synthesis of the fire ecology in the US forest ecosystems at the regional level. Two main pillars are analyzed across all ecoregions and forest types covered in the book, the drivers and linkages that form fire history and interactions initiated by recurring fire events, and the interdependence of fire with the forest ecosystem dynamics and humans.

Conclusions
The research, policy, and management tools available for managing fire as a disturbance and as prevention and mitigation treatment need cross-boundary revision. It is fundamental to realize the precise role of fire at present and to redefine the role of fire in the short and long term. This book is an invitation to change our perspective and methods towards these directions.
Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community
Authors: Gruninger Robert J. , Zhang Xiu Min , Smith Megan L. , Kung Limin , Vyas Diwakar , McGinn Sean M. , Kindermann Maik , Wang Min , Tan Zhi Liang , Beauchemin Karen A. 
Published: May 2022

Abstract
Background
The major greenhouse gas from ruminants is enteric methane (CH4) which in 2010, was estimated at 2.1 Gt of CO2 equivalent, accounting for 4.3% of global anthropogenic greenhouse gas emissions. There are extensive efforts being made around the world to develop CH4 mitigating inhibitors that specifically target rumen methanogens with the ultimate goal of reducing the environmental footprint of ruminant livestock production. This study examined the individual and combined effects of supplementing a high-forage diet (90% barley silage) fed to beef cattle with the investigational CH4 inhibitor 3-nitrooxypropanol (3-NOP) and canola oil (OIL) on the rumen microbial community in relation to enteric CH4 emissions and ruminal fermentation.

Results
3-NOP and OIL individually reduced enteric CH4 yield (g/kg dry matter intake) by 28.2% and 24.0%, respectively, and the effects were additive when used in combination (51.3% reduction). 3-NOP increased H2 emissions 37-fold, while co-administering 3-NOP and OIL increased H2 in the rumen 20-fold relative to the control diet. The inclusion of 3-NOP or OIL significantly reduced the diversity of the rumen microbiome. 3-NOP resulted in targeted changes in the microbiome decreasing the relative abundance of Methanobrevibacter and increasing the relative abundance of Bacteroidetes. The inclusion of OIL resulted in substantial changes to the microbial community that were associated with changes in ruminal volatile fatty acid concentration and gas production. OIL significantly reduced the abundance of protozoa and fiber-degrading microbes in the rumen but it did not selectively alter the abundance of rumen methanogens.

Conclusions
Our data provide a mechanistic understanding of CH4 inhibition by 3-NOP and OIL when offered alone and in combination to cattle fed a high forage diet. 3-NOP specifically targeted rumen methanogens and partly inhibited the hydrogenotrophic methanogenesis pathway, which increased H2 emissions and propionate molar proportion in rumen fluid. In contrast, OIL caused substantial changes in the rumen microbial community by indiscriminately altering the abundance of a range of rumen microbes, reducing the abundance of fibrolytic bacteria and protozoa, resulting in altered rumen fermentation. Importantly, our data suggest that co-administering CH4 inhibitors with distinct mechanisms of action can both enhance CH4 inhibition and provide alternative sinks to prevent excessive accumulation of ruminal H2.
Receptor of advanced glycation end product (RAGE) polymorphism and oxidative status in Hashimoto’s thyroiditis in Egyptian female patients: case control study
Authors: Mohamed Noura Mostafa , elfatah Azza H. Abd 
Published: May 2022

Abstract
Background
Hashimoto's thyroiditis is the most widespread autoimmune illness targeting a specific organ. "Redox homeostasis" is achieved when the production of Reactive Oxygen Species and their elimination are in balance. Advanced glycation end products (AGEs) are formed when glucose and/or α-oxaloaldehydes react non-enzymatically with the amino groups of lipids, proteins, and DNA. Nowadays, many studies are concerned with AGEs, the polymorphisms of their receptors, and their association with increased risk of HT. However, few studies investigated the role of receptors of advanced glycation end product (RAGE) SNP in Egyptian females.

Objective
The goals of this investigation were to ascertain whether oxidative stress plasma malondialdehyde (MDA) and total antioxidant capacity (TAC) were associated with HT, in addition, to assess the association of RAGE polymorphisms (− 374 T > A and the − 429 T > C and Gly82Ser) with HT.
Subject and methods.
Our case–control study has 80 patients enrolled who have newly been diagnosed with HT and 80 age and sex-matched healthy female controls. Each participant underwent a thorough medical history, physical examination, and laboratory investigations, which included Genotyping of RAGE Gly82Ser, − 374 T > A and − 429 T > C using polymerase chain reaction-restriction fragment length polymorphisms (PCR–RFLP).

Results
Chi-square revealed a significant association regarding the distribution of RAGE (− 374 T < C) genotypes TT and CC between patients and control (P = 0.04). Non-significant associations regarding the distribution of Gly82Ser genotypes Gly/Gly, Gly/Ser, Ser/Ser were found between patients and control (P = 0.5), and non-significant associations related to − 429 T > C gene polymorphism were revealed. In addition, patients with HT had higher MDA and lower TCA compared with controls.

Conclusion
The elevated MDA and decreased TAC as an antioxidant may be one of several risk factors associated with the prevalence of HT in individuals with the − 429 T > C RAGE mutation polymorphism that is associated with an increased risk of HT in Egyptian females.