bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of microneedling with 5% minoxidil topical solution combination therapy in treatment of androgenetic alopecia
Authors: Jia Lingling , Xiong Jiachao , Guo Rong , Li Yufei , Jiang Hua 
Published: July 2022

AbstractBackgroundMicroneedling (MN) therapy is one of minimal invasive operations with needles rolled over skin to puncturing the epidermis, and it is becoming a widely used treatment during various dermatological diseases includes androgenetic alopecia (AGA).ObjectiveThe purpose of the current study is to investigate the clinical observation and safety of MN combined with 5% minoxidil on triggering hair growth in AGA patients.Material and MethodsThis retrospective study has analyzed 18 AGA patients who were treated by MN in combination with 5% minoxidil topical solution between July 2021 and February 2022. All patients received six sessions of treatment under aseptic condition at an interval of 1 week. Assessment of hair regrowth was done at the baseline and 10 weeks by photography, investigator, and patient assessment global scoring table on clinical improvement, and the patient's final satisfaction was investigated.ResultsAccording to the standardized 7‐point scale, mean scores of investigator and participant assessments were 1.44 ± 0.61 and 1.66 ± 0.59, respectively, indicating that the hair appearance was considerably improved by MN combined with 5% minoxidil treatment. Fifteen patients (83.3%) were satisfied with the improvement in hair growth. No severe adverse events were noted in patients during and after the procedure.ConclusionThe combination of the length of 1.5 mm MN and 5% minoxidil in the treatment of AGA showed efficacy with high safety, which is worthy of clinical application.
Reflections on health among women in homelessness: A qualitative study
Authors: Kneck Åsa , Klarare Anna , Mattsson Elisabet , Salzmann‐Erikson Martin ,   in collaboration with Women Advisory Board for Inclusion Health
Published: July 2022

Accessible SummaryWhat is known on the subject?

Previous research reports that people in homelessness have poor physical and mental health and are excluded from society with risk for loneliness and social exclusion.

Women in homelessness face particularly vulnerable circumstances with significant risks of harm.

What the paper adds to existing knowledge?

Feelings of having a home is a basis for meeting physical, psychosocial, and existential needs related to health.

Being involved in authentic relationships and caring for others gives women in homelessness a sense of worth.

To be accepted by others and feeling like an equal was important for experiences of preserved dignity.

What are the implications for practice?

Nurses need to recognize and support women in homelessness for their capacity to heal and to experience health.

To regard women in homelessness as active health‐seekers, instead of passive victims, can improve women's experiences of care provided by mental health professionals.

Nurses can promote health by regarding women as resourceful and active, despite the fact that they live in homelessness.

AbstractIntroductionMental health issues are common among women in homelessness, alongside undertreated chronic physical conditions leading to serious and unnecessary complications. Even though homelessness and risks of impaired health have been researched, broader perspectives of health are absent.AimTo describe reflections on health among women with experiences of homelessness.MethodWe conducted 13 interviews with women in homelessness using researcher‐driven photograph elicitation. Together with an advisory board of women with lived experience of homelessness, researchers were guided by the DEPICT model for collaborative data analysis and performed a thematic analysis.FindingsWomen with experiences of homelessness emphasized three main resources for achieving health and well‐being: feelings of having a home, being involved in authentic relationships and experiences of preserved dignity.Implication for practiceHealthcare needs to integrate the perceived resources for health and well‐being when caring for women in homelessness. It is imperative since women will return to the healthcare setting only if they feel safe and secure, and only if dignity is preserved or restored. The results promote utilization of an integrative nursing approach; understanding that the health of women in homelessness is inseparable from their environment and social determinants for health, such as housing and social integration.
Uncertainty quantification of structural flexibility identified from input–output measurement data for reliability analysis
Authors: Li Panjie , Zhao Tongkuai , Zhang Jian , Wei Jiabei , Feng Maria Q. , Feng Dongming , Li Shengli 
Published: July 2022

AbstractIn contrast to the traditional uncertainty quantification of the modal parameters identified from the output‐only measurement data, this study develops an uncertainty propagation and quantification method for structural flexibility identified from input–output measurement data and then uses the derived flexibility matrix for structural reliability analysis. First, a novel procedure for variance estimations of the complete system matrix of the state‐space model is derived based on first‐order perturbation theory. Then, the mode scaling factors and integrated modal flexibility are subsequently derived and quantified. Finally, the predicted displacements and their uncertainty intervals are used to implement the reliability analysis in terms of the structural displacement serviceability limit state (SLS). The accuracies of the derived expressions are verified by a numerical example through the comparison of variance values estimated from Monte Carlo simulations. The simulation example also indicates that we can expect to identify structural parameters with higher reliability if the input force to the structure is measured, and the identified modal scaling and corresponding flexibility matrix undergo more uncertainty than those of the modal parameters. Finally, we apply the proposed uncertainty quantification method to a scaled three‐story frame structure to demonstrate its practicability. The proposed method bridges a gap between the identified structural flexibility and structural reliability analysis directly by the probability category.
Molecular investigation of the incidence of Candida auris infections at selected hospitals in Iran
Authors: Safari Fatemeh , Madani Mahboobeh , Kheirollahi Majid , Mirhendi Hossein 
Published: July 2022

AbstractBackgroundThe accurate occurrence rate of C. auris infections is still not clear, mainly due to the defects in detection and identification tools routinely used. In this study, we used conventional PCR and real‐time PCR assays for sensitive and specific detection/identification of C. auris from either yeast isolates or clinical specimens collected from various patients in different parts of Iran. Our survey is the first large‐scale study rating the incidence of C. auris infections in Iran.MethodsA total of 439 yeast isolates and 590 clinical specimens were screened by specific C. auris‐PCR, targeting the ITS region. The validity of positive samples was assessed by sequencing.ResultsFour out of 590 clinical specimens (0.68%) were positive by conventional PCR, while in real‐time PCR performed on 100 clinical samples, including those four samples positive in conventional samples, 6 samples were positive. A complete agreement of the identification of positive cases with sequencing results was documented. Among 439 culture isolates, none was positive for C. auris. After following up and resampling of the patients with positive PCR, only one specimen showed positive culture for C. auris, which was confirmed by sequencing.ConclusionC. auris is not a common cause of systemic or superficial fungal infections in Iran, and a few detected positive cases can be considered as a commensal, coloniser or infecting yeast which may potentially emerge in some clinical and therapeutical conditions. Mycological and phenotypical assays are not sensitive approaches for isolation/identification of C. auris, unless a specific and sensitive molecular‐based method is applied.
Evidence of chromatin and transcriptional dynamics for cold development in peach flower bud
Authors: Canton Monica , Forestan Cristian , Marconi Gianpiero , Carrera Esther , Bonghi Claudio , Varotto Serena 
Published: July 2022

Summary

In temperate zones, fruit trees regulate their annual growth cycle to seasonal environmental changes. During the cold season, growth is limited by both environmental and genetic factors. After the exposure to low temperature and fulfillment of chilling requirements, mild temperatures promote the growth and flowering. However, an insufficient chilling exposure may lead to nonuniform blooming, with a negative impact on fruit set.

To gain insights into flower development in the fruit tree buds, peach is an interesting model, the flower and vegetative bud being distinct organs. To understand how flower bud development is regulated, we integrated cytological observations and epigenetic and chromatin genome‐wide data with transcriptional changes to identify the main regulatory factors involved in flower development during chilling accumulation.

We demonstrated that growth cessation does not occur in peach flower buds during chilling accumulation, but that there are changes in transcript abundance of key genes of hormone metabolism and flower bud development, distribution of histone modifications (H3K4me3 and H3K27me3) and DNA methylation.

Altogether, our findings indicate that during the cold season the flower bud is in a nondormant state and that the chilling experience allows flower differentiation to be completed.
Analysis of Th‐cell subsets in local and systemic environments from experimental periodontitis rats
Authors: Yuan Yun , Zhang Hongming , Gu Qinhua , Xu Xinrui , Yu Runping , Huang Hui 
Published: July 2022

AbstractObjectives: The objective of this study was to explore the effect of periodontitis on Th‐cell subsets in local and systemic environments.Methods: A total of 32 male Sprague‒Dawley rats were randomly divided into periodontitis and control groups. Silk ligatures were applied to the mandibular first (M1) molars in the periodontitis group. Inflammation and alveolar bone loss around the M1 molars were analyzed by histological staining and microcomputed tomography. The mRNA expression of interferon‐γ (IFN‐γ), interleukin 4 (IL‐4), IL‐17, and IL‐10 in the gingiva was measured by qRT‐PCR. The proportions of Th1, Th2, Th17, and Treg cells in the submandibular lymph nodes, peripheral blood, and jaw bone marrow were tested using flow cytometry.Results: More inflammatory cells and alveolar bone resorption were found in the periodontitis group, with upregulated mRNA expression of IFN‐γ, IL‐17, and IL‐10. The proportion of Th1 and Th17 cells was significantly elevated in submandibular lymph nodes, and the proportion of Th1, Th2, and Th17 cells was significantly elevated in peripheral blood, while the proportion of Th1, Th17, and Treg cells was significantly elevated in jaw bone marrow in the periodontitis group.Conclusion: This study suggests that periodontitis affects the differentiation of Th‐cell subsets in both local and systemic environments, resulting in an increased proportion of proinflammatory cells.
A reappraisal of microbiome dysbiosis during experimental periodontitis
Authors: Arce Marion , Endo Natalia , Dutzan Nicolas , Abusleme Loreto 
Published: July 2022

AbstractPeriodontitis is a chronic inflammatory disease associated with the presence of dysbiotic microbial communities. Several studies interrogating periodontitis pathogenesis have utilized the murine ligature‐induced periodontitis (LIP) model and have further examined the ligature‐associated microbiome relying on 16S rRNA‐based sequencing techniques. However, it is often very challenging to compare microbial profiles across studies due to important differences in bioinformatic processing and databases used for taxonomic assignment. Thus, our study aim was to reanalyze microbiome sequencing datasets from studies utilizing the LIP model through a standardized bioinformatic analysis pipeline, generating a comprehensive overview of microbial dysbiosis during experimental periodontitis.We conducted a reanalysis of 16S rDNA gene sequencing datasets from nine published studies utilizing the LIP model. Reads were grouped according to the hypervariable region of the 16S rDNA gene amplified (V1‐V3 and V4), preprocessed, binned into operational taxonomic units and classified utilizing relevant databases. Alpha‐ and beta‐diversity analyses were conducted, along with relative abundance profiling of microbial communities.Our findings revealed similar microbial richness and diversity across studies and determined shifts in microbial community structure determined by periodontitis induction and study of origin. Clear variations in the relative abundance of bacterial taxa were observed starting on day 5 after ligation and onward, consistent with a distinct microbial composition during health and experimental periodontitis. We also uncovered differentially represented bacterial taxa across studies, dominating periodontal health and LIP‐associated communities.Collectively, this reanalysis provides a unified overview of microbial dysbiosis during the LIP model, providing new insights that aim to inform further studies dedicated to unraveling oral host–microbial interactions.
Protein interactome mapping of Porphyromonas gingivalis provides insights into the formation of the PorQ‐Z complex of the type IX secretion system
Authors: Gorasia Dhana G. , Veith Paul D. , Reynolds Eric C. 
Published: July 2022

AbstractPorphyromonas gingivalis is an anaerobic Gram‐negative human oral pathogen highly associated with the more severe forms of periodontal disease. Porphyromonas gingivalis utilises the type IX secretion system (T9SS) to transport ∼30 cargo proteins, including multiple virulence factors, to the cell surface. The T9SS is a multiprotein system consisting of at least 20 proteins, and recently, we characterised the protein interactome of these components. Similar to the T9SS, almost all biological processes are mediated through protein‒protein interactions (PPIs). Therefore, mapping PPIs is important to understand the biological functions of many proteins in P. gingivalis. Herein, we provide native migration profiles of over 1000 P. gingivalis proteins. Using the T9SS, we demonstrate that our dataset is a useful resource for identifying novel protein interactions. Using this dataset and further analysis of T9SS P. gingivalis mutants, we discover new mechanistic insights into the formation of the PorQ‐Z complex of the T9SS. This dataset is a valuable resource for studies of P. gingivalis.
Successful treatment of a child's pityriasis rubra pilaris (PRP) with ustekinumab and acitretin
Authors: Katharina Medek , Sylvia Selhofer , Matthias Buchner , Ulrich Mrowietz , Martin Laimer 
Published: July 2022

AbstractPityriasis rubra pilaris (PRP) is a rare inflammatory skin disease that occurs with phenotypic variability in adults of all ages as well as in children. Data on the treatment of PRP is limited. Here, we report a 5‐year‐old girl with widespread skin involvement and prominent palmoplantar hyperkeratosis who was initially treated for psoriasis. After reevaluation, a diagnosis of PRP was made, and the patient had an excellent therapeutic response to ustekinumab and acitretin.
Livedo racemosa associated with central venous catheter use in a newborn
Authors: Barsan Kaya Tuğba , Kaya Erdoğan Hilal , Güneş Damla , Aydemir Özge 
Published: July 2022

AbstractLivedo racemosa (LRac) refers to violaceous, red, or blue netlike mottling of the skin in an irregular pattern caused by circulatory abnormalities. Central venous catheters (CVC) are widely used in neonates who require intensive care to offer steady intravenous access. CVCs are covered with a hydrophilic polymer gel that reduces friction between the sheath and the vessel wall, thereby minimizing vascular spasm, irritation, and aneurysm formation. However, the introduction of foreign material into the vein carries a risk of embolization. Recently, LRac was reported as a cutaneous manifestation of hydrophilic polymer gel embolization (HPE) in adults. We present a case of LRac in a newborn that developed following CVC insertion and resolved spontaneously after the catheter was removed.
CD7 CAR T bridging to allo‐HSCT in R/R T‐ALL: A case report
Authors: Fanqiao Meng , Chen Xiuqiong , Ren Xiaotong , Li Lijuan , Wu Tong 
Published: July 2022

AbstractBackgroundRefractory/relapsed T‐cell acute lymphoblastic leukemia (R/R T‐ALL) is a hematological malignancy with a poor prognosis. The current treatment strategy has not benefited most patients, and the treatment methods are still being explored.Case presentationAn 8‐year‐old boy with R/R T‐ALL achieved CR after multiple chemotherapies, followed by the first allo‐HSCT. Unfortunately, 1 year and 3 months later, he relapsed. After recurrence, the patient underwent multiple chemotherapies, but the NOTCH1 gene and MRD were still positive. FCM and immunohistochemistry revealed abnormally high expression of CD7, so we considered bridging the second allo‐HSCT after CD7 CAR T‐cells treatment. The patient has low toxic and side effects and is still in CR, findings from this case report have more important therapeutic significance for R/R T‐ALL.ConclusionIn conclusion, CD7 CAR T‐cells bridging to allo‐HSCT is a safe and effective approach for R/R T‐ALL, resulting in durable CR and longer survival.
THE NEGATIVELY BIASED MANDARIN BELIEF VERB yĭwéi*
Authors: Glass Lelia 
Published: July 2022

Abstract
The Mandarin belief verb yĭwéi strongly suggests that the belief it embeds is wrong or questionable. Based on original data, I propose that this sense of negative bias stems from a postsupposition that the reported belief must not be accepted in the Common Ground following an update with yĭwéi. When a belief is reported using a neutral, nonfactive verb such as rènwéi ‘think,’ it is possible for the content of that belief to become Common Ground if the belief or belief‐holder are considered reliable; but the postsupposition of yĭwéi explicitly blocks such an inference, giving rise to its negative bias. By heading off a potential inference, this postsupposition is further argued to serve a function common to other proposed postsuppositions in the literature. Zooming out, this investigation illuminates the complex calculations triggered by belief reports in discourse, and the linguistic resources used to guide them.
Calcification and osseous metaplasia of the meibomian glands of a horse
Authors: Gunsalus Kerry , Taylor Ryan , Croix Noelle La 
Published: July 2022

AbstractObjectiveTo report the clinical history, surgical management, and histologic findings of meibomian gland calcification and osseous metaplasia in a horse.Animal StudiedA 21‐year‐old Selle Français gelding presented with a 9 months history of blepharitis, blepharospasm, and epiphora affecting the right eye. The horse was diagnosed with meibomianitis and impaction with associated granulomas, but not treated surgically for 6 years.ProceduresPhysical and ophthalmic examinations, and systemic bloodwork were performed. A diamond burr debridement was performed on a corneal ulceration, and meibomian gland nodules were excised and examined histologically.ResultsMultiple firm concretions were associated with the palpebral conjunctiva of each eyelid. The largest nodule (5 × 4 × 10 mm) was excised from the lower right eyelid. Histology revealed dilated meibomian ducts with mineralized inspissated secretions surrounded by fibrosis and osseous metaplasia. Following nodule excision and corneal ulcer resolution, no blepharospasm or epiphora recurred for a follow‐up period of 15 months.ConclusionCalcification and osseous metaplasia of the meibomian glands was identified in a Selle Français gelding. It is suspected that inspissated meibomian secretions led to a local granulomatous reaction with secondary dystrophic calcification and osseous metaplasia.
Laparoscopic vertical sleeve gastrectomy in felines: A cadaveric feasibility study and experimental case series in two cats
Authors: Buote Nicole J. , Porter Ian , Loftus John , Cummings Bethany , Dakin Gregory F. 
Published: July 2022

AbstractObjectiveTo evaluate the feasibility of laparoscopic vertical sleeve gastrectomy (LVSG) in feline cadavers using endoscopic stapling equipment and report clinical outcomes in two live feline subjects.Study designCadaveric study and experimental case series.AnimalsTen feline cadavers; two feline subjects.MethodsLVSG technique was refined on feline cadavers and included retraction of the liver, dissection of the stomach, assessment of proper location for gastrectomy via stapling, and leak testing. Appropriateness of gastrectomy, gastrectomy %, surgical times and complications were recorded. The procedure was performed on two live feline subjects, and they were followed for 4 months to report surgical complications.ResultsLVSG was completed in 9/10 cadavers and both live patients. Stenosis at the incisura was recorded in 2/9 cadavers. No obvious leaks were seen in the 8 cadavers that were tested or either live patient. The mean surgical time for all cadaver procedures and live patients was 110.4 and 115 minutes, respectively. Mean weight of resected cadaver stomach was 10 g and the mean % of the total stomach weight resected was 27.6%. No intra‐ or postoperative surgical complications occurred in the live subjects.ConclusionLVSG technique appears feasible and safe for use in live patients.Clinical relevance: This LVSG technique may be safely used for partial gastric resection in cats. Further studies are necessary to determine if it is effective at reversing the effects of obesity and diabetes in this population.
Mitogenomics supports the monophyly of Mysidacea and Peracarida (Malacostraca)
Authors: Höpel Christoph G. , Yeo Darren , Grams Markus , Meier Rudolf , Richter Stefan 
Published: July 2022

AbstractHere, we provide the first complete mitochondrial genomes for two higher taxa of Peracarida, Lophogastrida and Stygiomysida. We examined Lophogaster typicus as a representative of Lophogastrida and Spelaeomysis bottazzii as a representative of Stygiomysida. Both mitogenomes have all typical metazoan genes (13 protein‐coding genes, two ribosomal RNA genes and 22 transfer RNAs). The mitogenomes have a length of 15,076 bp in L. typicus and 14,806 bp in S. bottazzii. Gene order differs markedly from the hypothetical pancrustacean/malacostracan ground pattern in both species, and in L. typicus, all genes were encoded on the heavy strand. This is the first time this is described for a crustacean. We also reconstruct eumalacostracan phylogenies using a data set consisting of 98 species based on alignments comprising all protein‐encoding genes as well as the protein‐encoding genes and the two ribosomal RNAs. We find support for the monophyly of Mysidacea based on species from all three higher taxa (Mysida, Lophogastrida, Stygiomysida). Moreover, our analyses also support a monophyletic Peracarida with Amphipoda or Amphipoda + Mysidacea as the sister group to the remaining Peracarida.