bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ocean acidification affects the expression of neuroplasticity and neuromodulation markers in seabream
Authors: Costa Rita A. , Olvera Aurora , Power Deborah M. , Velez Zélia 
Published: February 2022

ABSTRACT
A possible explanation for acidification-induced changes in fish behaviour is that acidification interferes with neurogenesis and modifies the plasticity of neuronal circuitry in the brain. We tested the effects on the olfactory system and brain of gilthead seabream (Sparus aurata) to 4 weeks' exposure to ocean acidification (OA). Olfactory epithelium (OE) morphology changed shortly after OA exposure and persisted over the 4 weeks. Expression of genes related to olfactory transduction, neuronal excitability, synaptic plasticity, GABAergic innervation, and cell proliferation were unchanged in the OE and olfactory bulb (OB) after 4 weeks' exposure. Short-term changes in the ionic content of plasma and extradural fluid (EDF) returned to control levels after 4 weeks' exposure, except for [Cl−], which remained elevated. This suggests that, in general, there is an early physiological response to OA and by 4 weeks a new homeostatic status is achieved. However, expression of genes involved in proliferation, differentiation and survival of undifferentiated neurons were modified in the brain. In the same brain areas, expression of thyroid hormone signalling genes was altered suggesting modifications in the thyroid-system may be linked to the changes in neuroplasticity and neurogenesis. Overall, the results of the current study are consistent with and effect of OA on neuroplasticity.
The complete mitogenome sequence of clam (Corbicula fluminea)
Authors: Luo Qing , Pi Jie , Tang Yangxin , Zeng Cong , Li Deliang 
Published: February 2022

The global invasion of the genus Corbicula has caused serious ecological and economic problems. The species of Corbicula fluminea stands out amongst the greater part of freshwater invaders around the world. Here, we sequenced the entire mitogenome of the Corbicula fluminea, which were gathered from Dongting Lake, in the Hunan province of central China. The circular genome is 17,073 bp long. The raw reads were obtained from the platform of Illumina HiSeq 2500, and assembled by the MITObim method followed by alignments to related species. The entire dataset was deposited at the NCBI Short Read Archive via accession number SRR14692229 and NCBI GenBank with accession number MZ231034.1.
Recommendations for the formatting of Variant Call Format (VCF) files to make plant genotyping data FAIR
Authors: Beier Sebastian , Fiebig Anne , Pommier Cyril , Liyanage Isuru , Lange Matthias , Kersey Paul J. , Weise Stephan , Finkers Richard , Koylass Baron , Cezard Timothee , Courtot Mélanie , Contreras-Moreira Bruno , Naamati Guy , Dyer Sarah , Scholz Uwe 
Published: February 2022

In this opinion article, we discuss the formatting of files from (plant) genotyping studies, in particular the formatting of (meta-) data in Variant Call Format (VCF) files. The flexibility of the VCF format specification facilitates its use as a generic interchange format across domains but can lead to inconsistency between files in the presentation of metadata. To enable fully autonomous machine actionable data flow, generic elements need to be further specified. We strongly support the merits of the FAIR principles and see the need to facilitate them also through technical implementation specifications. VCF files are an established standard for the exchange and publication of genotyping data. Other data formats are also used to capture variant call data (for example, the HapMap format and the gVCF format), but none currently have the reach of VCF. In VCF, only the sites of variation are described, whereas in gVCF, all positions are listed, and confidence values are also provided. For the sake of simplicity, we will only discuss VCF and our recommendations for its use. However, the part of the VCF standard relating to metadata (as opposed to the actual variant calls) defines a syntactic format but no vocabulary, unique identifier or recommended content. In practice, often only sparse (if any) descriptive metadata is included. When descriptive metadata is provided, proprietary metadata fields are frequently added that have not been agreed upon within the community which may limit long-term and comprehensive interoperability. To address this, we propose recommendations for supplying and encoding metadata, focusing on use cases from the plant sciences. We expect there to be overlap, but also divergence, with the needs of other domains.
Emvolio - A battery operated portable refrigerator preserves biochemical and haematological integrity of biological samples in preclinical studies
Authors: Maity Swastika , Aakriti Jaya , Manandhar Suman , Anchan Sharad B , Bhat Ashlesh , Shetty Mayur U , Nayak Yogendra 
Published: February 2022

Background: Emvolio is a non-medical device, indigenously developed portable refrigeration for maintaining the internal temperature 2–8˚C. The Indian Patent Office has granted patent for applications such as preservation and transport of medicines, vaccines, food, beverages, dairy etc. Further, use of Emvolio can be utilized in transport and store biologicals to preserve their biochemical and cellular integrity. The objective of this study was to evaluate the biochemical and haematological integrity of biological samples such as rat blood, serum and liver. Methods: The steady temperature was maintained inside the Emvolio, and it was compared to that of thermocol and polypropylene boxes aided with frozen gel packs. The blood and liver samples were isolated from Wistar rats and kept in Emvolio, thermocol and polypropylene boxes for 10 hrs, and the temperature was monitored. The blood parameters, namely red blood cells (RBC), white blood cells (WBC), platelets, haematocrit, haemoglobin, mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC) and red cell distribution width (RDW), serum parameters like alanine transaminase, alkaline phosphatase, total protein, albumin, creatine kinase, blood urea nitrogen and liver parameters like superoxide dismutase (SOD), glutathione (GSH), catalase were estimated and compared. Results: Emvolio maintained a constant inner temperature range of 2–8˚C, whereas a significant temperature variation was seen in thermocol and polypropylene boxes. There was no significant deviation in the parameters tested when samples were kept in Emvolio for six hours compared to the zero hour readings. In contrast, there was a significant deviation among the parameters for the samples kept in thermocol and polypropylene boxes for six hours compared to zero hour parameters. Conclusions: Emvolio maintained constant temperature and preserved the biological integrity of rat blood, serum and liver. Thus, Emvolio can be efficiently used as a biological sample carrier, especially in preclinical studies.
Education research is still the hardest science: a proposal for improving its trustworthiness and usability
Authors: Fischman Gustavo , Amrein-Beardsley Audrey , McBride-Schreiner Stephanie 
Published: February 2022

In this essay, we argue that colleges of education, particularly those at research-intensive institutions, favor simplistic notions of scholarly impact and that this trend has concerning implications for the field, for researchers, and for the public at large. After describing the challenges and shortcomings of the current models of research assessment in education, we outline an alternative proposal in which trustworthiness and usability of research would complement traditional metrics of scholarly relevance. This proposal encourages a twofold approach to research assessment that involves (1) a more thorough analysis of the limitations and problems generated by the use of simplistic notions of scholarly impact, and (2) a commitment to the implementation of more equitable systems based on a broader range of assessment measures to assess faculty research contributions.
Economic evaluations of maternal health interventions: a scoping review
Authors: Eddy Katherine E. , Eggleston Alexander , Chim Sher Ting , Zahroh Rana Islamiah , Sebastian Elizabeth , Bykersma Chloe , McDonald Steve , Homer Caroline S. E. , Scott Nick , Chou Doris , Oladapo Olufemi T. , Vogel Joshua P. 
Published: February 2022

Background Evidence on the affordability and cost-effectiveness of interventions is critical to decision-making for clinical practice guidelines and development of national health policies. This study aimed to develop a repository of primary economic evaluations to support global maternal health guideline development and provide insights into the body of research conducted in this field. Methods A scoping review was conducted to identify and map available economic evaluations of maternal health interventions. We searched six databases (NHS Economic Evaluation Database, EconLit, PubMed, Embase, CINAHL and PsycInfo) on 20 November 2020 with no date, setting or language restrictions. Two authors assessed eligibility and extracted data independently. Included studies were categorised by subpopulation of women, level of care, intervention type, mechanism, and period, economic evaluation type and perspective, and whether the intervention is currently recommended by the World Health Organization. Frequency analysis was used to determine prevalence of parameters. Results In total 923 studies conducted in 72 countries were included. Most studies were conducted in high-income country settings (71.8%). Over half pertained to a general population of pregnant women, with the remainder focused on specific subgroups, such as women with preterm birth (6.2%) or those undergoing caesarean section (5.5%). The most common interventions of interest related to non-obstetric infections (23.9%), labour and childbirth care (17.0%), and obstetric complications (15.7%). Few studies addressed the major causes of maternal deaths globally. Over a third (36.5%) of studies were cost-utility analyses, 1.4% were cost-benefit analyses and the remainder were cost-effectiveness analyses. Conclusions This review provides a navigable, consolidated resource of economic evaluations in maternal health. We identified a clear evidence gap regarding economic evaluations of maternal health interventions in low- and middle-income countries. Future economic research should focus on interventions to address major drivers of maternal morbidity and mortality in these settings.
Demoulding process assessment of elastomers in micro-textured moulds
Authors: Liarte Elias , Zambrano Valentina , A. Gracia Leticia , Amor José Ignacio , Borro Marcos , Hernández-Gascón Belén 
Published: February 2022

Background: Micro-texturing is an increasingly used technique that aims at improving the functional behaviour of components during their useful life, and it is applied in different industrial manufacturing processes for different purposes, such as reducing friction on dynamic rubber seals for pneumatic equipment, among others. Micro-texturing is produced on polymer components by transfer from the mould and might critically increase the adhesion and friction between the moulded rubber part with the mould, provoking issues during demoulding, both on the mould itself and on the rubber part. The mould design, the coating release agent applied to the mould surface, and the operational parameters of the moulding/demoulding process, are fundamental aspects to avoid problems and guarantee a correct texture transfer during the demoulding process. Methods: In this work, the lack of knowledge about demoulding processes was addressed with an in-house test rig and a robust experimental procedure to measure demoulding forces (DFs) as well as the final quality of the moulded part, between thermoset polymers and moulds. After the characterization of several Sol-Gel coating formulations (inorganic; hybrid) the influence of several parameters was analysed experimentally, i.e.: Sol-Gel efficiency, texture effects, pattern geometry, roughness and material compound. Results: The results obtained from the experimental studies revealed that texture depth is the most critical geometrical parameter, showing high scatter among the selected compounds. Finally, the experimental results were used to compute a model through reduced order modelling (ROM) technique for the prediction of DFs. Conclusions: The characterization of DFs in a laboratory, with a specific device operated by a universal testing machine (UTM), provided valuable information that allows a fast and optimized introduction of texturing in rubber components. Selection of a novel Sol-Gel coating and the use of the ROM technique contributed to speed up implementation for mass production.
Effects of vasopressor agents on the development of pressure ulcers in critically ill patients: a systematic review
Authors: McEvoy Natalie , Patton Declan , Avsar Pinar , Curley Ger , Kearney Cathal , Clarke Jennifer , Moore Zena 
Published: February 2022

Objective: The primary objective of this systematic review was to determine the effect of vasopressor agents on the development of pressure ulcers (PUs) among critically ill patients in intensive care units (ICUs). The secondary outcome of interest was length of stay in the ICU. Method: A systematic review was undertaken using the databases searched: Medline, Embase, CINAHL and The Cochrane Library. Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were used to formulate the review. Data were extracted using a predesigned data extraction table and analysed as appropriate using RevMan. Quality appraisal was undertaken using the EBL Critical Appraisal Tool. Results: The inclusion criteria were met by 13 studies. Two studies provided sufficient data to compare the number of patients who developed a PU with and without the use of vasopressors. Consistently, within these two studies, being treated with a vasopressor increased the likelihood of PU development. RevMan analysis identified that shorter duration of administration of vasopressors was associated with less PU development (mean difference (MD) 65.97 hours, 95% confidence interval (CI): 43.47–88.47; p=0.0001). Further, a lower dose of vasopressors was also associated with less PU development (MD: 8.76μg/min, 95% CI: 6.06–11.46; p<0.00001). Mean length of stay increased by 11.46 days for those with a PU compared to those without a PU (MD: 11.46 days; 95% CI: 7.10–15.82; p<0.00001). The overall validities of the studies varied between 45–90%, meaning that there is potential for bias within all the included studies. Conclusion: Vasopressor agents can contribute to the development of PUs in critically ill patients in ICUs. Prolonged ICU stay was also associated with pressure ulcers in this specific patient group. Given the risk of bias within the included studies, further studies are needed to validate the findings of this review paper.
Passively Q-switched Tm:YAP laser with a tantalum aluminum carbide saturable absorber
Authors: Zong Tianhao , Li Linjun , Gao Qiong , Liu Bangzheng , Yang Xining , Yang Yuqiang , Cui Haoyu 
Published: February 2022

Tantalum aluminum carbide (

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l

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) phase ceramic (MAX) material has attracted much attention because of its high conductivity, high strength, corrosion resistance, and good optical properties. However, there are too few reports on lasers with

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l

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-based saturable absorbers (SAs). We prepared and characterized a

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-based SA whose nonlinear absorption performances were achieved at a 2 µm waveband range and which was used in a passively

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-switched (PQS) Tm:YAP laser. In the PQS mode, a maximum average output power of 0.78 W was achieved with the central output wavelength of 1991.86 nm from a PQS Tm:YAP laser, corresponding to a pulse duration of 926 ns at 143.8 kHz.
Early INSURE Therapy Reduces CPAP Failure in Late Preterm Newborns with Respiratory Distress Syndrome
Authors: Alarcon Olave Maria Camila , Gómez-Ochoa Sergio Alejandro , Jerez-Torra Kihara Alejandra , Martínez-González Paula Liseth , Sarmiento-Villamizar Diego Felipe , Díaz-Martínez Luis Alfonso , Africano-León Martha Lucía , Rojas-Devia Mario Augusto , Pérez-Vera Luis Alfonso 
Published: February 2022

Background: INSURE (Intubation, Surfactant administration, and Extubation) therapy is controversial in managing respiratory distress syndrome (RDS) in late preterm newborns. This study aims to determine whether the use of the INSURE in late preterm infants with RDS is associated with improved outcomes compared to similar infants managed with CPAP alone. Methods: A retrospective cohort study compared two different neonatal care units with two different treatments of RDS in late preterm infants in Bucaramanga, Colombia. One cohort used selective bubble CPAP and rescue surfactant, the second cohort used selective bubble CPAP and INSURE within the first hour of life. We included all the newborns with gestational age between 33 - 366/7 weeks, born between 2012 to 2017, that developed early RDS and were treated with CPAP. Results: We recruited 208 patients (57 CPAP and rescue surfactant and 151 CPAP + INSURE). Early INSURE was reported in 117 patients (56.3 %). INSURE therapy was associated with a reduced risk of CPAP failure (RR = 0.50; 95 % CI 0.26 - 0.98); this effect was evident only when surfactant was administered within the first two hours of life (RR = 0.29; 95 % CI 0.12 - 0.69). Early INSURE was associated with a decreased risk of pneumothorax (RR = 0.07; 95 % CI 0.01 - 0.77) and pulmonary hypertension (RR = 0.34; 95 % CI 0.14 - 0.78). Conclusions: Early INSURE therapy was associated with a reduced incidence of CPAP failure, pneumothorax, and pulmonary hypertension in late preterm infants with moderate to severe RDS. Large, well-powered randomized controlled trials are needed to confirm these observations, but its use is supported by studies with similar results in more premature infants.
DETEKSI GEN HAEMOLISIN PADA Aeromonas hydrophila MENGGUNAKAN METODE PCR (Polymerase Chain Reaction) DI LABORATORIUM UJI BRPI, SUKAMANDI
Authors: Artati Diah , Lubis Dini Sahfitri 
Published: February 2022

Penyakit pada ikan merupakan salah satu kendala yang sering terjadi dan ikut berperan menurunkan angka keberhasilan para pembudidaya ikan. Salah satu jenis penyakit yang sering dijumpai pada organisme budidaya adalah penyakit bakterial yang disebabkan oleh serangan bakteri Aeromonas hydrophila. Berkembangnya metode identifikasi berbasis molekuler yang lebih cepat dengan tingkat sensitivitas dan spesifitas yang tinggi diharapkan mampu meminimalisir kerugian yang ditimbulkan. Kegiatan ini dilakukan menggunakan metode PCR dengan tujuan untuk mengetahui kemampuan primer AHH1 dan primer A16S dalam mendeteksi keberadaan gen haemolisin pada bakteri A. hydrophila serta mengkonfirmasi penggunaan ATCC 35654 sebagai referensi jaminan mutu pengujian pada kegiatan identifikasi bakteri A.hydrophila di Laboratorium Mikrobiologi BRPI, Sukamandi. Haemolisin merupakan lipid dan protein yang dapat menyebabkan lisis pada sel darah merah dengan cara menghancurkan membran sel. Gen 16S rRNA merupakan asam ribonukleat pengkode ribosom 16S. Gen tersebut memiliki sejumlah basa yang disebut sebagai hypervariable region yang merupakan ciri khas yang mampu membedakan tiap organisme. ATCC 35654 yang diamplifikasi menggunakan primer AHH1 dan A16S positif terdeteksi sebagai A. hydrophila yang membawa gen haemolisin.
Pengaruh Pemberian Pakan Rucah dan Pakan Pellet Terhadap Pertumbuhan Juvenil Ikan Kerapu Bebek (Chromileptes altivelis)
Authors: Wamnebo Ikhsan , Ali Ulat Muhammad 
Published: February 2022

Penelitian ini bertujuan untuk mengetahui Pengaruh pemberian pakan rucah dan pakan pellet terhadap pertumbuhan dan kelangsungan hidup juvenil ikan kerapu bebek (Chromileptes altivelis). Penelitian dilaksanakan di Balai Budidaya Laut Ambon. Penelitian terdiri dari 2 perlakuan yakni: Perlakuan A (pakan rucah) dan Perlakuan B (pakan pellet). Hasil penelitian menunjukkan bahwa tingkat pertumbuhan dan kelangsungan hidup juvenil ikan kerapu bebek (Chromileptes altivelis) yang diberi pakan rucah menmperlihatkan bahwa panjang rata-rata akhir adalah 10,56 cm dan berat rata-rata akhir 11,52 gram, sedangkan panjang rata-rata akhir ikan kerapu bebek yang diberi pakan pellet adalah 9,43 cm dan berat rata-rata akhir 8,44 gram. Tingkat kelangsungan hidup ikan kerapu bebek baik yang diberikan pakan pellet maupun pakan rucah menghasilkan angka kelangsungan yang tinggi yakni 100%.
HUBUNGAN PANJANG-BERAT DAN NISBAH KELAMIN LOBSTER BATU (Panulirus penicillatus) DI PANTAI SELATAN YOGYAKARTA
Authors: Larasati Rakhma Fitria , Setyobudi Eko , Suadi Suadi 
Published: February 2022

ABSTRAK Lobster (Panulirus spp.) adalah komoditas perikanan penting dan ekonomis tinggi. Spesies yang paling sering dan dominan tertangkap adalah lobster batu. Meningkatnya permintaan lobster menjadikan nelayan melakukan kegiatan penangkapan secara terus menerus. Penelitian ini bertujuan untuk menganalisis hubungan panjang-berat dan rasio jenis kelamin (sex ratio) lobster batu (Panulirus penicillatus). Penelitian dilakukan pada Februari 2017 sampai dengan Agustus 2017 di pantai selatan Yogyakarta. Kajian stok lobster dilakukan melalui survei yang meliputi metode deskriptif, observasi dan wawancara. Pemilihan lokasi pengambilan sampel dilakukan secara terpilih (purposive) yaitu pantai Ngrenehan, Baron, Drini dan Tepus. Total sampel lobster batu yang diperoleh sepanjang penelitian sejumlah 546 ekor, yang terdiri dari lobster jantan 289 ekor (53%) dan lobster betina 257 ekor (47%). Hasil penelitian menyatakan bahwa pola hubungan panjang dan berat lobster batu memiliki sifat allometrik negative dengan persamaan W= 0,004 L 2,577 pada lobster jantan dan W = 0,007 CL 2,481 pada lobster betina. Perbandingan nisbah kelamin lobster batu jantan dan betina adalah 1,12 : 1. ABSTRACTLobster (Panulirus spp.) is an important and economically high fishery commodity. The most frequent and dominant species caught is rock lobster. The increasing demand for lobsters makes fishermen conduct continuous catching activities. The study aims to analyze the relationship of length-weight and sex ratio of rock lobster (Panulirus penicillatus). The research was conducted from February 2017 to August 2017 in southern coast of Yogyakarta. The study of lobster stocks was conducted through surveys that included descriptive methods, observations and interviews. The sampling location was chosen purposively, namely Ngrenehan, Baron, Drini and Tepus. Total samples obtained throughout the study amounted to 546 double-spined rock lobster, consisting of 289 males (53%) and 257 females (47%). The results stated that the length and weight relationship pattern of rock lobsters is negative allometric, with the equation W= 0,004 L 2,577in males lobster and W = 0,007 CL 2,481 in females lobster. The sex ratio double-spined rock lobster between males and females lobster is 1,12 : 1.