bluuebunny/crossref_metadata_2025_split huggingface dataset:
The C4EB study—Transvamix (10% THC / 5% CBD) to treat chronic pain in epidermolysis bullosa: A protocol for an explorative randomized, placebo controlled, and double blind intervention crossover study
Authors: Schräder Nicholas H. B. , Duipmans José C. , Renken Remco J. , Sörös Peter , Vermeulen Karin M. , Bolling Maria C. , Wolff André P. 
Published: December 2022

Patients with the genetic blistering skin condition epidermolysis bullosa (EB) report severe pain as a consequence of skin and mucous membrane lesions including blisters, wounds, and scars. Adequate symptom alleviation is not often achieved using conventional pharmacologic interventions. Finding novel approaches to pain care in EB is imperative to improve the quality of life of patients living with EB. There are several anecdotal reports on the use of cannabinoid-based medicines (CBMs) by EB patients to reduce the burden of symptoms. However, controlled clinical investigations assessing these reported effects are lacking. As the pain quality “unpleasantness” delineates EB pain, we hypothesize the modulation of affective pain processing in the brain by way of intervention with CBMs comprising the cannabinoids Δ-9-tetrahydrocannabinol and cannabidiol—objectified by functional magnetic resonance imaging (fMRI). The C4EB study is an investigator-initiated, single-centre, randomized, double-blind, placebo-controlled and crossover trial. Adult patients with the diagnosis epidermolysis bullosa, reporting chronic pain will be eligible to participate. Following baseline measurements, participants will be randomized to receive the sublingually administered interventions placebo and Transvamix® in forward or reversed orders, each for two weeks and separated by a washout. The primary outcome is the difference in numeric rating scale pain scores between grouped interventions, using affective descriptors within the Short-form McGill Pain Questionnaire-2. Secondary outcomes include pain self-efficacy, concomitant analgesic medication-use and adverse events. Additionally, fMRI will be employed to assess brain connectivity related to neuroanatomic pain circuits at baseline, placebo and Transvamix® interventions. The study was approved by the ethical committee at the University Medical Center of Groningen in the Netherlands. Results will be submitted for publication in a peer-reviewed journal.
Trial registration number: Netherlands Trial Register: NL9347 (Acronym: C4EB).
Tracking development assistance for health from India to low- and middle-income countries, 2009–2020
Authors: Moitra Modhurima , Patel Nishali K. , Cogswell Ian , Chanana Dweep I. , Maddison Emilie , Simpson Kyle , Stutzman Hayley , Zhao Yingxi , Tsakalos Golsum , Dieleman Joseph , Micah Angela E. 
Published: December 2022

Background
Development assistance for health (DAH) is an important source of financing for health for many low-income and some middle-income countries. Most DAH has predominantly been contributed by high-income countries. However, in the context of economic progress and changing global priorities, DAH contributions from countries of the Global South such as India have gained importance. In this paper, we estimate DAH contributed by India between 2009 and 2020.

Methods
We leveraged data from budgetary documents, databases, and financial reports of the Ministry of External Affairs and multilateral organizations to estimate DAH contributions. The proportions of development assistance that go towards health in major recipient countries were estimated and reported by recipient country and year.

Results
Between 2009 and 2020, DAH contributed by India to bilateral and multilateral partners totaled $206.0 million. South Asian countries including Bangladesh, Bhutan, Nepal, Sri Lanka, and Myanmar received the most DAH from India. DAH contributed relative to DAH received ranged from 1.42% in 2009 to 5.26% in 2018, the latest year with country-level data. Health focus areas prioritized by India included technical training and innovation, health care infrastructure support, and supply of medications and medical equipment.

Conclusion
India is an important development partner to many countries–particularly to those in the South Asian region. India’s DAH allocation strategy prioritizes contributions toward neighboring countries in the South Asia region in several health focus areas. Detailed project-level data are needed to estimate DAH contributions from India with greater precision and accuracy.
Perceived discomfort and neuromuscular fatigue during long-duration real driving with different car seats
Authors: Lecocq Mathieu , Lantoine Pascaline , Bougard Clément , Allègre Jean-Marc , Bauvineau Laurent , González Damián , Bourdin Christophe , Marqueste Tanguy , Dousset Erick 
Published: December 2022

Introduction
Identification of the seat features that could improve driving experience is a main issue for automotive companies.

Objective
Long duration real driving sessions were performed to assess the effect of three seats (soft–S1, firm–S2 and suspended–S3) on perceived discomfort and neuromuscular fatigue (NMF).

Materials & methods
For each seat, the muscular activity of bilateral Trapezius Descendens (TD), Erector Spinae (ES) and Multifidus (MF) muscles of twenty-one participants was recorded during real driving sessions of 3-hours each lasting approximately 3 hours and following the same itinerary. During each driving session, participants were also regularly asked to self-evaluate their level of whole-body and local discomfort. In addition, an endurance static test (EST) was performed before (ESTpre) and after (ESTpost) each driving session to assess the seat effect on physical capacity.

Results
Whole-body discomfort increased with driving time for all seats, but this increase became significant latter for S3. The highest scores of local discomfort occurred for neck and lower back. Contrary to S1 and S2, the duration of ESTpost was not significantly lower compared to ESTpre with the S3. Interestingly, muscular activity of S1 remained stable throughout the driving task which could be attributed to sustained muscular contraction, while muscular recruitment adjustments occurred for S2 and S3 from 1H00 of driving. This muscular compensation concerns mostly the right side for S2 and S3 but with different profiles. On the left side, the muscular adjustments concern only the MF with S2 and the ES with S3.

Conclusion
Overall, our results demonstrated that S3 could be considered as the most suitable seat to delay discomfort and NMF appearance.
Prevalence of prediabetes and type 2 diabetes mellitus in south and southeast Asian women with history of gestational diabetes mellitus: Systematic review and meta-analysis
Authors: Shivashri Chockalingam , Hannah Wesley , Deepa Mohan , Ghebremichael-Weldeselassie Yonas , Anjana Ranjit Mohan , Uma Ram , Mohan Viswanathan , Saravanan Ponnusamy 
Published: December 2022

Background
The burden of Gestational Diabetes Mellitus (GDM) is very high in south Asia (SA) and southeast Asia (SEA). Thus, there is a need to understand the prevalence and risk factors for developing prediabetes and type 2 diabetes mellitus (T2DM) postpartum, in this high-risk population.

Aim
To conduct a systematic review and meta-analysis to estimate the prevalence of prediabetes and T2DM among the women with history of GDM in SA and SEA.

Methods
A comprehensive literature search was performed in the following databases: Medline, EMBASE, Web of Knowledge and CINHAL till December 2021. Studies that had reported greater than six weeks of postpartum follow-up were included. The pooled prevalence of diabetes and prediabetes were estimated by random effects meta-analysis model and I2 statistic was used to assess heterogeneity.

Results
Meta-analysis of 13 studies revealed that the prevalence of prediabetes and T2DM in post-GDM women were 25.9% (95%CI 18.94 to 33.51) and 29.9% (95%CI 17.02 to 44.57) respectively. Women with history of GDM from SA and SEA seem to have higher risk of developing T2DM than women without GDM (RR 13.2, 95%CI 9.52 to 18.29, p<0.001). The subgroup analysis showed a rise in the prevalence of T2DM with increasing duration of follow-up.

Conclusion
The conversion to T2DM and prediabetes is very high among women with history of GDM in SA and SEA. This highlights the need for follow-up of GDM women for early identification of dysglycemia and to plan interventions to prevent/delay the progression to T2DM.
Factors associated with acute malnutrition among children aged 6–59 months in Haiti, Burkina Faso and Madagascar: A pooled analysis
Authors: Nassur Ali-Mohamed , Daanouni Oussama , Luc Gwenaelle , Humphreys Alexandra , Blanarova Lenka , Heymsfield Grace , Kouassi Firmin , Kangas Suvi T. , N’Diaye Dieynaba S. 
Published: December 2022

Background
Acute malnutrition is one of the main causes of morbidity and mortality among children under 5 years worldwide, and Action Contre la Faim (ACF) aims to address its causes and consequences. To better tailor humanitarian programs, ACF conducts standardized contextual studies called Link NCAs (Nutrition Causal Analysis), to identify factors associated with severe acute malnutrition (SAM) and moderate acute malnutrition (MAM). Data from three Link NCAs performed in 2018 and 2019 in Haiti, Burkina Faso and Madagascar were used to explore the prevalence of malnutrition by different indicators and associated risk factors among children aged 6–59 months.

Methods
Cross-sectional data, collected via household surveys applying two-stage cluster sampling, were pooled to build a sample of 1,356 children. Recommended anthropometric thresholds were used to define SAM (Weight-for-Height Z-score (WHZ) <-3 or Mid-upper Arm Circumference (MUAC) <115 mm and/or presence oedema), MAM (-3≤WHZ<-2 or 115≤MUAC<125 mm) and global acute malnutrition GAM (SAM or MAM) among children. Multivariate analyses for each anthropometric indicator were performed using logistic mixed models and adjusting for potential confounders.

Results
The prevalence of acute malnutrition was the highest in Madagascar. The risk of having GAM and MAM varied across countries, while the risk of having SAM varied across clusters. Being male, suffering from diarrhea, and having unwashed face and hands, were significantly associated with GAM by WHZ with adjusted odds ratio of 1.9 [95%Confidence interval (CI):1.1–3.2], 1.7 (95%CI: 1.0–3.1) and 1.9 (95%CI: 1.0–3.6) respectively. These factors were also associated with MAM by WHZ. None of the studied factors was significantly associated with SAM, which could be due to a small sample size.

Conclusion
These results obtained from a large sample contribute to the evidence of the factors associated with undernutrition in children aged 6–59 months. Further research with larger sample sizes is needed to confirm these results.
PAISAGEM: CONCEPÇÕES, ASPECTOS MORFOLÓGICOS E SIGNIFICADOS
Authors: Silva Vicente de Paulo de Paulo da 
Published: December 2022

O objetivo deste trabalho é discutir acerca do conceito de paisagem sob as perspectivas morfológica
e de seu significado. Dessa forma é importante que se busque, inicialmente, entender
o conceito sob as formas que se nos apresentam para, em seguida, analisá-lo do ponto de
vista dos diversos significados que possam ser atribuídos a essa categoria. A paisagem apresenta
várias dimensões privilegiadas por várias matrizes epistemológicas. Sendo assim, ela
tem uma dimensão morfológica e uma dimensão funcional, uma dimensão espacial e uma
dimensão histórica (CORRÊA E ROSENDAHL, 1998). De acordo com estes autores a dimensão
morfológica é definida como um conjunto de formas criadas pela natureza, bem como pela
ação humana, sendo que, ao apresentar relações entre as diversas partes, define-se a sua
dimensão funcional. Essa ação humana, ao longo do tempo, definirá a dimensão histórica,
enquanto que o fato desta ocorrer em certa área da superfície da terra atribui-lhe a dimensão
espacial. Enfim, o fato de ser portadora de significados, expressando valores, crenças, mitos e
utopias, confere à paisagem a sua dimensão simbólica.
Wideband Fabric Antenna for Ultra Wideband Applications using for Medical Applications
Authors: Bhavani S. , Shanmuganantham T. 
Published: December 2022

Traditional cancer detection imaging techniques suffer high costs, high false negatives, high false positives,and pain. The microwave imaging techniques overcome the limitations, which depend primarily on antenna design. If an antenna is wearable and implantable, the imaging system gives better results with less pain and cost. A wide band fabric antenna that operates at the ultra-wideband frequency with a low specific absorption rate (SAR) on breast phantom is verified. The proposed design has Jeans material as a substrate and the copper patch as a radiating element. The patch is designed in a circular shape with an M-type slot to suppress the spurious modes. The designed antenna model is commonly used for monitoring microwave imaging and has dimensions of 28X30X1.6 mm3. The proposed antenna design covers 2.3-8 GHz frequency with a broadside radiation pattern. The gain over the operating frequency is about 2.3-4.5 dB, and the efficiency is 55 %–79 %. The antenna model was designed and simulated in CST microwave studio. The performance of an antenna is tested on breast cancer to detect the presence of tumor cells in the breast. The antenna analysis on the phantom was done by considering the tumor location and corresponding results are presented. By varying the sizes of the tumor the antenna performance is analysed. The simulated SAR values of the proposed antenna design on breast phantom are under the limits of FCC.
Molecular insights into endolysosomal microcompartment formation and maintenance
Authors: Kümmel Daniel , Herrmann Eric , Langemeyer Lars , Ungermann Christian 
Published: December 2022

Abstract
The endolysosomal system of eukaryotic cells has a key role in the homeostasis of the plasma membrane, in signaling and nutrient uptake, and is abused by viruses and pathogens for entry. Endocytosis of plasma membrane proteins results in vesicles, which fuse with the early endosome. If destined for lysosomal degradation, these proteins are packaged into intraluminal vesicles, converting an early endosome to a late endosome, which finally fuses with the lysosome. Each of these organelles has a unique membrane surface composition, which can form segmented membrane microcompartments by membrane contact sites or fission proteins. Furthermore, these organelles are in continuous exchange due to fission and fusion events. The underlying machinery, which maintains organelle identity along the pathway, is regulated by signaling processes. Here, we will focus on the Rab5 and Rab7 GTPases of early and late endosomes. As molecular switches, Rabs depend on activating guanine nucleotide exchange factors (GEFs). Over the last years, we characterized the Rab7 GEF, the Mon1-Ccz1 (MC1) complex, and key Rab7 effectors, the HOPS complex and retromer. Structural and functional analyses of these complexes lead to a molecular understanding of their function in the context of organelle biogenesis.
Rapid diagnosis of intra-amniotic infection using nanopore-based sequencing
Authors: Chaemsaithong Piya , Romero Roberto , Pongchaikul Pisut , Vivithanaporn Pornpun , Lertrut Waranyu , Jaovisidha Adithep , Mongkolsuk Paninee , Nitayanon Perapon , Pongsuktavorn Khontawan , Kamlungkuea Threebhorn , Jung Eunjung , Suksai Manaphat , Singhsnaeh Arunee , Jenjaroenpun Piroon , Thaipisuttikul Iyarit , Wongsurawat Thidathip 
Published: December 2022

Abstract

Objectives
Early diagnosis and treatment of intra-amniotic infection is crucial. Rapid pathogen identification allows for a definite diagnosis and enables proper management. We determined whether the 16S amplicon sequencing performed by a nanopore sequencing technique make possible rapid bacterial identification at the species level in intra-amniotic infection.

Methods
Five cases of confirmed intra-amniotic infection, determined by either cultivation or 16S rDNA polymerase chain reaction (PCR) Sanger sequencing, and 10 cases of women who underwent mid-trimester genetic amniocentesis were included. DNA was extracted from amniotic fluid and PCR was performed on the full-length 16S rDNA. Nanopore sequencing was performed. The results derived from nanopore sequencing were compared with those derived from cultivation and Sanger sequencing methods.

Results
Bacteria were successfully detected from amniotic fluid using nanopore sequencing in all cases of intra-amniotic infection. Nanopore sequencing identified additional bacterial species and polymicrobial infections. All patients who underwent a mid-trimester amniocentesis had negative cultures, negative 16S PCR Sanger sequencing and nanopore sequencing. Identification of the microorganisms using nanopore sequencing technique at the bacterial species level was achieved within 5–9 h from DNA extraction.

Conclusions
This is the first study demonstrating that the nanopore sequencing technique is capable of rapid diagnosis of intra-amniotic infection using fresh amniotic fluid samples.
Translational regulation by Hfq–Crc assemblies emerges from polymorphic ribonucleoprotein folding
Authors: Dendooven Tom , Sonnleitner Elisabeth , Bläsi Udo , Luisi Ben F 
Published: December 2022

AbstractThe widely occurring bacterial RNA chaperone Hfq is a key factor in the post‐transcriptional control of hundreds of genes in Pseudomonas aeruginosa. How this broadly acting protein can contribute to the regulatory requirements of many different genes remains puzzling. Here, we describe cryo‐EM structures of higher order assemblies formed by Hfq and its partner protein Crc on control regions of different P. aeruginosa target mRNAs. Our results show that these assemblies have mRNA‐specific quaternary architectures resulting from the combination of multivalent protein–protein interfaces and recognition of patterns in the RNA sequence. The structural polymorphism of these ribonucleoprotein assemblies enables selective translational repression of many different target mRNAs. This system elucidates how highly complex regulatory pathways can evolve with a minimal economy of proteinogenic components in combination with RNA sequence and fold.
Sports Team Participation and Vaping Among High School Students: 2015–2019
Authors: Rapoport Eli , Zhu Meng’ou , Pham Duy , Keim Sarah A. , Adesman Andrew 
Published: December 2022

Background and Objectives
Electronic vapor products (EVPs) have gained popularity among adolescents despite the health risks. This study aimed to evaluate whether sports team participation, a well-established protective factor against cigarette use, is similarly associated with decreased EVP use.

Methods
This cross-sectional study analyzed the 2015–2019 Youth Risk Behavior Survey cohorts. Survey-weighted logistic regressions investigated associations between sports team participation and past 30-day exclusive cigarette use, exclusive EVP use, and dual cigarette/EVP use among US high school students, adjusting for sex, grade, and survey year.

Results
The analytic cohort included 16 790 sports team participants (1.7% exclusive cigarette users, 18.3% exclusive EVP users, 5.5% dual users) and 13 972 nonparticipants (3.1% exclusive cigarette users, 13.4% exclusive EVP users, 7.6% dual users). Sports team participation was associated with lower odds of cigarette use (adjusted odds ratio [aOR], 0.58; 95% confidence interval [CI], 0.48-0.71) and dual use (aOR, 0.74; 95% CI, 0.63-0.88) and higher odds of EVP use (aOR, 1.39; 95% CI, 1.25-1.54). Among exclusive cigarette users and exclusive EVP users, sports team participation was associated with lower odds of frequent (≥20 days in the past month) than intermittent (1-19 days in the past month) cigarette use (aOR, 0.30; 95% CI, 0.19-0.49) and EVP use (aOR, 0.74; 95% CI, 0.61-0.91), respectively.

Conclusions
Our findings suggest that risk profiles for cigarette and EVP use differ with respect to sports team participation. Given the health risks associated with EVP use, aggressive efforts must be taken to educate student athletes about the health risks of EVP use.
Identification of Long-term Care Challenges in Europe
Authors: Drzazga Beata 
Published: December 2022

Background: Changes in demographic structures and especially the dynamic ageing of the population lead to an increased demand for care services. Finding ways to provide them efficiently has become one of the challenges for developed countries. The choice of the research issue is a consequence of recognising the fact that in recent years it has increasingly emerged as an area of practical and theoretical reflection in many scientific disciplines, including management and health sciences.
Research objectives: The aim of the study is to characterize characterising selected aspects of the organisations and functioning of long-term care in EU countries, describing the related initiatives taken by the European Commission and identifying the challenges faced by decision-makers and managers in the said service sector.
Research design and methods: The paper is based on the analysis of European documents, Eurostat database and literature review. It provides numerical analysis of current and prospective variables such as: average population age in EU 28, projected population age structure, population aged 65+, long-term care expenditure.
Results: Analysis of the available statistical data unambiguously confirms that increasing life expectancy, combined with low birth rates, leads to a significant quantitative increase in the proportion of older people in the overall population structure of EU countries. There is also no internationally accepted and standardised definition of what constitutes long-term care and thus no possibility of reliably identifying needs in this area. In many Member States, long-term care systems are characterised by horizontal interdependencies with health care and social care systems. Consequently, they may and do apply different criteria for long-term care eligibility, needs assessment, social protection scope and the catalogue of services and benefits offered.
Conclusions: The evolving social structures, the increasing introduction of artificial intelligence solutions and the situation after COVID-19 are changing the conditions of long-term care functioning, hence the reflection on the directions of these transformations is essential to properly formulate public policy practices.
The probability of developing a tumor in the small intestine neocyst
Authors: Kеln A. A. , Petrosyan G. S. , Kupchin A. V. , Lykov A. V. , Lebedev A. V. , Volynkina L. V. 
Published: December 2022

In clinical practice and scientific literature, there is very little data on the occurrence of tumors in the urinary intestinal reservoirs in patients after radical cystectomy with cystoplasty. A characteristic feature of such tumors is a long latent period before the formation of a relapse from 4 to 32 years.The article describes a clinical case of a 1951 patient with a tumor of a small intestinal neocyst. From the patient’s history, there is a long-term hematuria (for 2 years) after radical cystectomy with ileocystoplasty. The intensity of hematuria increased to the formation of profuse with the development of severe anemia. During the examination according to magnetic resonance imaging, computed tomography, ultrasound and invasive diagnostic examinations, no tumor was detected. The patient underwent a diagnostic laparotomy, a tumor was found in the area of ureteral-reservoir anastomosis of the small intestine neocyst. Resection of the small intestine reservoir and ureteral transplantation was performed. The morphological picture of the removed tumor is urothelial cancer of moderate differentiation.Secondary tumors of the small intestinal urinary reservoirs are rare and have the structure of adenocarcinoma. The mechanisms of their occurrence have not been fully studied at the moment. Dynamic monitoring of patients after radical cystectomy and ileocystoplasty should be carried out in a specialized center.