bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histone demethylase KDM5B catalyzed H3K4me3 demethylation to promote differentiation of bone marrow mesenchymal stem cells into cardiomyocytes
Authors: Wang Zhen , Zhong Chenlu , Li Hongxiao 
Published: February 2022

Abstract
BACKGROUND: Studies have shown that histone H3 methylation is involved in regulating the differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs). KDM5B can specifically reduce the level of histone 3 lysine 4 trimethylation (H3K4me3), thereby activating the expression of related genes and participating in biological processes such as cell differentiation, embryonic development and tumor formation. Whether KDM5B is involved in the regulation of BMSCs differentiation into cardiomyocytes through the above manner has not been reported.OBJECTIVE: To investigate the effect of KDM5B on the induction and differentiation of swine BMSCs into myocardial cells in vitro.METHODS: Swine bone marrow BMSCs were isolated and cultured, and the overexpression, interference expression and blank vector of KMD5B were constructed and transfected by lentivirus. BMSCs was induced to differentiate into cardiomyocytes by 5-azacytidine (5-AZA) in vitro, and the differentiation efficiency was compared by immunofluorescence, RT-PCR, Western Blot and whole-cell patch clamp detection.RESULT: Compared with the control group, the expression levels of histone H3K4me3 and pluripotency gene Nanog in KDM5B overexpression group were significantly decreased, while the expression level of key myocardial gene HCN4 and myocardial marker gene α-Actin and cTNT were significantly increased, and the Na+ current density on the surface of differentiated myocardial cell membrane was significantly increased. Meanwhile, the corresponding results of the KDM5B silent expression group were just opposite. CONCLUSIONS: It indicated that enhanced KDM5B expression could promote the differentiation of BMSCs into cardiomyocytes and improve the differentiation efficiency by controlling H3K4 methylation levels.
A Collective Control Scheme to Solve Time-varying Formation Control Problem of Multiple Autonomous Underwater Vehicles With Time Delay
Authors: Jia Yongnan , Zhang Weicun 
Published: February 2022

Abstract
Due to the limitation of complexity and uncertainty of the underwater environment, the related technologies of autonomous underwater vehicles(AUVs) develop slowly. Therefore, an ingenious solution characterized by low cost, convenient operation, and low individual intelligence is urgently required. Inspired from these collective behaviours of gregarious creatures in nature, the coordination control problem of multiple AUVs is endowed with new research significance to complete complex underwater operational tasks. This paper aims to propose a general control scheme to solve the time-varying formation control problem of multiple AUVs that take into account the communication time delay. Firstly, a complete six-degrees-of-freedom dynamical model is applied instead of the real AUVs in the following theoretical analysis and simulation verification. Then, a metric-based nearest neighbour interacted rule is introduced to build the communication network of the system. Periodic sampling technology and zero-order hold loop are adopted to simplify the communication problem of time delay. Based on the above dynamical model and communication mechanism, a distributed collective control protocol is proposed to enable these AUVs asymptotically converge to a desired geometrical configuration on the condition that the initial communication network is undirected and connected. During the evolutionary process, no collision happens between any two AUVs. The formation configuration can be maintained until a simple switching controller works for the configuration transformation tasks. Finally, the simulation results proved the effectiveness of the above collective control scheme and visually exhibited the three-dimensional dynamical evolutionary process.
The Effect Of Femoral Nerve Block Versus Adductor Canal Block On The Quality Of Early Recovery After Revision Total Knee Arthroplasty, A Retrospective Study
Authors: AlJa'bari Aboud , Sheikh Khalid AL 
Published: February 2022

Abstract
Background: Multimodal pain analgesia strategies are common in perioperative management of total knee arthroplasty (TKA), although the role of adductor canal blocks (ACB) versus femoral nerve block on early postoperative recovery for revision knee surgery is not investigated.Aim: The purpose of this study is to independently evaluate the effect of ACB on short-term postoperative outcomes including (1) length of stay (LOS), (2) postoperative narcotic utilization, and (3) function with physical therapy in revision TKA.Methods: We retrospectively identified a cohort study of consecutive 40 patients from January 2021 to July 2021 who had undergone unilateral revision TKA using a single-shot ACB (19 patients) vs femoral nerve block (21 patients) under spinal anesthesia (hyperbaric 0.5% Marcaine 2.5 ml and 20 microgram fentanyl) in addition to a standardized multimodal pain analgesia protocol. These 2 groups were compared using independent sample t-tests with primary end points of interest being distance ambulated after surgery, and inpatient narcotic use.Results: Quadriceps strength was better preserved in adductor group than in femoral group. Walking meters and going upstairs were better results in adductor group. IV morphine consumption within the first 48 hours period were less in adductor group comparing to femoral group. Conclusion: Adductor nerve block showed better early recovery in revision TKA when comparing to femoral nerve block (FNB).
What Are The Determinants Of Parametrial Invasion In Patients With Early Stage Cervical Cancer: A Cross Sectional Study
Authors: Hoorshad Niloufar , Zamani Narges , SheikhHasani Shahrzad , Poopak Amirhossein , Sharifi Amirsina 
Published: February 2022

Abstract
Background: There was an increase in number of patients presented with early-stage cervical cancer (CC). Tumors with favorable pathological features might be candidates for less radical surgery.Methods: We retrospectively reviewed 700 patients with histologically confirmed CC between January 2011 and March 2020. Chi-square, Fisher's exact tests and multivariate logistic regression analysis were used to assess relations between parametrial involvement (PI) and clinic-pathological variables.Results: Total number of 132 patients with stage IA to IIA were eligible to participate. Squamous cell carcinoma was reported in 100 (75.8%) patients, adenocarcinoma and other tumor pathologies were found in 24(18.2%) and 8(6.1%), respectively. Considering the FIGO stage, 11 (8.4%) patients had IA, 111 (83%%) IB and 10 (7.6%) IIA. Nine patients (6.8%) had PI on permanent pathologic report. Univariate analysis demonstrated that following variables were statistically different between patients with and without PI: age ≥ 50, tumor size ≥ 3cm, lower segment involvement, poorly differentiated pathology, deep stromal invasion, pelvic lymph node, lympho-vascular involvement and positive surgical margin (all p values < 0.05). Among these variables only tumor size ≥ 3 cm (OR: 2.1, 95% CI: 1.11-4.16, p value: 0.02), deep stromal invasion (OR: 2.2, 95% CI: 1.9-7.43, p value: 0.02) and positive surgical margin (OR: 5.1, 95% CI: 3.97-11.15, p value: 0.008) were independent risk factor of PI in multivariate analysis.Conclusions: Early stage CC can be surgically approached in a more conservative manner if patients have tumor size < 3 cm and do not have deep stromal invasion in conization.
Combination of a New Metaheuristic Algorithm Based on Cooperative Grouper Fish - Octopus and DBSCAN Algorithm to Automatic Clustering
Authors: Balavand Alireza 
Published: February 2022

Abstract
Density-based spatial clustering of applications with noise (DBSCAN) has been used to cluster data with arbitrary shapes which clustering is done based on the density among objects in data. Given that DBSCAN is a proper tool for identifying outliers and clustering non-convex data, it can be used for automatic clustering of non-convex data and covered the weakness of most automatic clustering algorithms in not recognizing non-convex clusters. So, in this paper, a new automatic clustering algorithm is introduced which is a combination of DBSCAN and grouper fish - octopus (GFO) algorithm. GFO-DBSCAN finds the best number of clusters in two main steps in an iterative manner. In the first step, the values of \(esp\) and \(minpts\) are generated by GFO algorithm and in the second step, the clustering of data is performed using DBSCAN algorithm with \(eps\) and \(minpts\) that are generated in the previous step. After each clustering, using correct data labels, and cluster centroids, the Calinski-Harabasz (CH) index is calculated. Finally, after passing some iterations of GFO, the best number of clusters is reported. In this study, three categories of data are used to measure the performance of the GFO-DBSCAN algorithm. Also, DBSCAN is compared with ACDE, DCPSO, and GCUK algorithms. According to the results, GFO-DBSCAN has achieved the optimal number of clusters in most data and has outperformed other well-known algorithms.
Shifting clinical relevance from ‘what is the matter to what matters’ utilising Legitimation Code Theory. A comparative investigation of medical students’ social accountability perceptions.
Authors: Whittenbury Kingsley James 
Published: February 2022

Abstract
Pedagogy for socially accountable medical professionalism lacks systematic integration of relevant social and biomedical facts. A sociological gaze and change in medical discourse may be transformative. A mixed methods study compared perceptions of social justice in healthcare of forty medical student participants from diverse social positions enrolled in two Australian medical schools. The ‘capability to aspire’ to prosocial professionalism links Sen’s capability theory and Bourdieu’s theory of practice to compare outsiders and insiders in a traditional and a socially accountable program. Comparison of First and Final Year cohorts aimed to identify the regulative discourses of each program. Demographic, short answer and multi-choice questions, and semi-structured audio-recorded interview tested participants’ aspirations, knowledges and reasoning related to social accountability. All cohorts supported social justice learning and agreed Indigenous, refugee and migrant groups were most disadvantaged in Australian health care. Final Year participants had greater awareness of health system shortfalls in social accountability, citing professional discourses lacking equity awareness, unfair patient responsibilisation, system overload and mismatch to Indigenous health needs. Less intention to practice in areas of health need was shown by participants with zero and multiple devalorised social identities than those with single equity identities. A common finding was inability to modify clinical reasoning to recognise what matters for equity needs, making ‘health equity’ a threshold concept. Critical reflexivity on human contexts of illness production was best realised by outsiders, unrelated to program. International participants held stronger perceptions of mismatch between the Australian health system and Indigenous health needs. Results inform a socio-science approach to learning health equity that builds on students’ prosocial values, social identities, and meaning-making of social contexts. A re-contextualising principle to validate health equity perspectives in medical pedagogy, and knowledge management system to integrate social and medical discourses, are proposed. The potential benefits of biosemiotic, edusemiotic and semioethic approaches to the humanising turn in medical education are discussed. Legitimation Code Theory provides a means to systematise diverse discourses in medical education. Biosemiotic concepts of lifeworld and dialogical communication may further cultivate students’ sociological and moral imagination of health production, and creativity for socially accountable solutions to emergent illness.
Lithospheric Based Eco-decontamination and Fate Elucidation of Fungicide Fluopyram Containing Benzamides Background
Authors: Naeem Hamna , Ahmad Khuram Shahzad 
Published: February 2022

Abstract
Fungicide Fluopyram (FLP) containing benzamides class has been scrutinized for the first time for its ecotoxicity and environmental lithosphere stationed fate from various climatic zones. Fate-decisive research inclusive of examining FLP's response in perspective of alteration via adsorption/desorption and disintegration in hydrolytic, photolytic, and bio-degradative procedures. Sorptive interactions determination was done by the batch equilibration approach and indicated its dependency on the chemico-physical properties of the soils. Fragile adsorptive interactions between soils and FLP molecules were established, as indicated by physio-sorption and a fragile exothermic response indicated by negative Gibbs free energy values (-8 to -15.3 kJ mol-1). The soil with the maximal adsorption, Kd(ads) =8.6 µg mL−1, was procured to have a significant organic matter content (1.30 %), resulting in a C-type isotherm that was scrutinized using a linear and Freundlich model. The analytical techniques, inclusive of ultraviolet-visible spectrophotometric and high-performance liquid chromatography procedures have been endeavored to examine FLP fate elucidation. Hydrolysis, photolysis, and biodegradation tests procured minimum halflives of 7.45, 1.29, and 5.77 days while the most remarkable were 10.6, 7.96, and 10.6 days, respectively. The results demonstrated that FLP displayed medium binding and thus low persistence in the tested soils while being extremely susceptible to transformative mechanisms. Current research can be embraced to include further augmentation of these degradative ways and to develop realistic methods for environmental cleanup through natural routes.
Financial Access of Adult People with Disability to Health Services: Rural Population Study from Iran
Authors: Hosseini-Shokouh Sayyed-Morteza , Shams Lida , nasiri Taha , Darvish Tahere 
Published: February 2022

Abstract
Background People with disabilities (PWDs) account for a significant percentage of the world's population, with a higher prevalence in less developed countries. Access to healthcare services is the main component of health systems performance, with lower access for PWDs living in rural areas. The current study aimed to investigate PWD's access to healthcare services in rural areas of Iran and, secondly, factors that contribute to this issue.Methods Following a cross-sectional design, the current descriptive-analytical study is performed in the north of Iran. Using the quota sampling technique, 471 PWDs were recruited. Data were collected using a valid and reliable questionnaire, covering three dimensions of access, by face-to-face interview. Data analysis was administered using central tendency indicators and multiply regression by SPSS version 17. Statistical significance was considered when p-value<0.05.Results The mean score of PWD's access to healthcare services for dimensions of utilization, availability, and affordability was 8.91(±6.86), 14.54(±2.3), and 51.91(±8.78), indicating very low, low, and moderate levels of access. All three regression models were significant (P<0.05), and variables of gender, age, marital status, education level, residence status, the income of the household head, receiving financial aid, and house area showed a significant effect (P<0.05).Conclusion This study demonstrated the seriousness of paying attention to PWD's financial access to healthcare services, particularly in rural areas of Iran. Hence, policymakers should better focus on this problem, mainly regarding accessibility and utilization and factors that result in inequalities.
Neutrophil Infiltration Induces Myocardial Injury In COVID-19 Post-Mortem Cases
Authors: Han Yaling , Zhang Quanyu , Zhang Huarong , Yan Xiaowei , Ma Sicong , Yao Xiaohong , Shi Yu , Ping Yifang , Cao Mianfu , Peng Chengfei , Wang Shuai , Luo Min , Yan Chenghui , Zhang Shuyang , Han Yaling , Bian Xiuwu 
Published: February 2022

Abstract
Objectives: The pathological features of severe cardiac injury induced by COVID-19 and relevant clinical features is unknown.Methods: This autopsy cohort study, including hearts from 26 deceased patients hospitalized in intensive care unit due to COVID-19, was conducted at four sites in Wuhan, China. Cases were divided into neutrophil-infiltration group and no-neutrophil group according to histopathological identification of neutrophilic infiltrates or not.Results: Among 26 cases, four cases had active myocarditis with histopathological examination. All cases with myocarditis accompanied with extensive neutrophil infiltration, while cases without myocarditis did not. Detection rates of interleukin-6 (100% vs 4.6%) and tumor necrosis factor-a (100% vs 31.8%) in neutrophil-infiltration group were significantly higher compared to no-neutrophil group (p<0.05 for both). At admission, patients with neutrophil infiltration in myocardium had significantly higher baseline values of aspartate aminotransferase, D dimer and high-sensitivity C reactive protein compared to other 22 patients (p<0.05 for all). During hospitalization, patients with neutrophil infiltration had a significantly higher maximum of creatine kinase (CK)-MB (median 280.0 vs 38.7IU/L, p=0.04), and a quantitatively higher top Troponin I (median 1.112 vs 0.220ng/ml, p=0.56) than patients without neutrophil infiltration. Conclusions: In hearts from deceased patients with severe COVID-19, active myocarditis was commonly infiltrated with neutrophils. Cases with neutrophil-infiltrated myocarditis had a series of severe abnormal laboratory tests at admission, and a high maximum of CK-MB during hospitalization. Role of neutrophil on severe heart injury and even systemic condition in COVID-19 should be emphasized.
BMI1 Promotes Cholangiocarcinoma Progression and Correlates with Antitumor Immunity in An Exosome-Dependent Manner
Authors: Liu Zengli , Hu Chunxiao , Zheng Lijie , Liu Jialiang , Li Kangshuai , Li Xingyong , Wang Yue , Mu Wentao , Chen Tianli , Shi Anda , Qiu Bo , Zhang Xin , Zhang Zongli 
Published: February 2022

Abstract
Background: Cholangiocarcinoma (CCA) is a class of malignant tumors originating from bile duct epithelial cells. Due to difficult early diagnosis and limited treatment, the prognosis of CCA is extremely poor. BMI1 is dysregulated in many human malignancies. However, the prognostic significance and oncogenic role of BMI1 in cholangiocarcinoma (CCA) are not well elucidated. Methods: In the present study, we investigated its clinical importance and the potential mechanisms in the progression of CCA. We detected BMI1 expression in a large CCA cohort. We demonstrated BMI1 was substantially upregulated in CCA tissues and was identified as an independent prognostic biomarker of CCA. Moreover, overexpression of BMI1 promoted CCA proliferation, migration, and invasion. And, BMI1 knockdown could inhibit proliferation and metastases of CCA in vitro and in vitro/vivo validation. Interestingly, we found that CCA-derived exosomes contain BMI1 proteins, which can transfer BMI1 between CCA cells. The unique BMI1-containing exosomes promote CCA proliferation and metastasis through autocrine/paracrine mechanisms. In addition, we demonstrated that BMI1 inhibits CD8+T cell-recruiting chemokines by promoting repressive H2A ubiquitination in CCA cells. Conclusions: BMI1 is an unfavorable prognostic biomarker of CCA. Our data demonstrate a novel function of BMI1 in CCA tumorigenesis and metastasis mediated by exosomes. Besides, BMI1 inhibition may augment immune checkpoint blockade to inhibit tumor progression by activating cell-intrinsic immunity of CCA
Adult and Pediatric All-Terrain Vehicle (ATV) Injury Patterns and Outcomes in a Community Trauma Center
Authors: Foote Christopher , Doan Xuan-Lan , Vanier Cheryl , Palacio Carlos 
Published: February 2022

Abstract
Background
All-terrain vehicle (ATV) crashes result in severe morbidity in trauma. Limited data on these injury patterns come from large urban academic centers, but studies show increased use of ATVs in small rural communities with fewer resources, where these injuries are more likely to be treated. This study uses injury patterns to determine impact on community trauma systems based on length of stay.
Methods
The trauma registry of a level II trauma center was reviewed for ATV crash patients from January 2015 to December 2020. Injury type and frequency were grouped by proportion and 95% confidence interval based on ‘score’ method, and co-incidences were first screened with Fisher’s exact test, with significant p-value<0.01. Factors influencing length of stay in hospital/ICU were analyzed in a linear model and log-transformed dependent variable with significance based on sequential nested likelihood ratio analysis. Descriptive statistics were back transformed.
Results
Out of 7,787 trauma patients admitted during the study, 127 were involved in ATV crashes with 62 adults (age>18) and 65 pediatric patients. Injuries to skin/soft tissue and extremities were most common in both adult (68% and 42%, p<0.001) and pediatric (56% and 38%, p<0.001). Paired injuries included head bleeds with skull fractures (p<0.001) in both adults and pediatrics, pediatrics alone with spine and rib fractures (p=0.01). Length of stay in hospital increased for extremity injuries in both groups (mean (SD); adults 5.8 (2.4) vs 3.0 (2.2) days and pediatrics 4.0 (2.2) vs 2.6 (2.0) days, P=0.048). Pediatric patients had shorter overall hospital length of stay compared to adults (mean (SD); 3.1 (2.1) vs 3.9 (2.4) days respectively, P=0.009). Mortality was low with only a single adult death.
Conclusions
The findings from our retrospective study among ATV-related injuries on a community level II trauma center demonstrates skin/soft tissue injuries and extremity injuries were most common. We can also predict prolonged hospital length of stay in those with extremity injuries and those older than 18 years of age, requiring greater resources with longer hospital stay. No factors were predictive of longer ICU stays and mortality was very low with a single adult death in our population.
Antibacterial Activity of Essential Oils Extracted From the Unique Chinese Spices Cassia Bark, Bay Fruits, and Cloves
Authors: Jiang Chunling , Meng Jing , Ou Jie , Xie Qingchao , Pan Yingjie , Zhao Yong , Liu Haiquan 
Published: February 2022

Abstract
Spices are widely used in daily life such as diet and have certain activity. Especially in China, spices have been mainly used as condiments for thousands of years in order to improve the sensory quality of food; in addition, they and their derivatives can also be used as preservatives. In this study, three species with Chinese characteristics unique and widely used by the public were selected: cassia bark (bark of Cinnamomum camphoraPresl), bay fruits (Laurus nobilis), and cloves (Syzygiumaromaticum). The main components and antibacterial ability of these three spices were analyzed by simulated extraction method. Through headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) analysis, it was determined that the main active compounds in the essential oils of cassia bark, bay fruits and cloves were cinnamaldehyde (78.11%), cinnamaldehyde (61.78%), and eugenol (75.23%), respectively. The agar plate diffusion test and the simulated food culture medium experiment confirmed that the essential oils extracted from the three flavors have antibacterial effects on Vibrio parahaemolyticus, Listeria monocytogenes and 4 kinds of Listeria. The antibacterial activity of different strains has different optimal extraction conditions. Generally speaking, cinnamon essential oil has the strongest antibacterial activity, while laurel fruit has the lowest antibacterial activity. The study proved the antibacterial activity of these three Chinese-specific spices, and provided some new ideas and methods for the subsequent research and preparation of natural food additives and food antibacterial agents.