bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychological risk indicators for peri‐implantitis: A cross‐sectional study
Authors: Strooker Hans , de Waal Yvonne Catharina Maria , Bildt Miriam Margot 
Published: May 2022

AbstractAimThe aim of this analytical cross‐sectional study was to evaluate the association between peri‐implantitis and psychological distress, and potentially related/mediating factors such as general health, bruxism, and lifestyle factors.Materials and MethodsPatients who received dental implants at a private practice in the Netherlands between January 2011 and January 2014 were recalled on a 5‐year clinical and radiographic follow‐up examination. Presence of peri‐implantitis was examined, and patients completed questionnaires measuring psychological distress (Symptom Checklist [SCL]‐90), bruxism, general health, and lifestyle factors. Associations between the self‐reported factors and peri‐implantitis were analysed with univariate and multivariate logistic regression models.ResultsA total of 230 patients (with 347 implants) were included in the analysis. Prevalence of (mild to severe) peri‐implantitis was 30% (69 patients). Variables that showed a significant univariable association with peri‐implantitis (p < .10) were the SCL‐90 subdomain depression, smoking, current medical treatment, and lung problems. In the multivariate regression analysis, depression was the only variable that was significantly associated with peri‐implantitis (p < .05).ConclusionsThe presence of depressive symptoms is a risk indicator for peri‐implantitis. Recognizing the potential negative impact of depressive symptoms may allow for better identification of high‐risk patients.
Ipriflavone suppresses NLRP3 inflammasome activation in host response to biomaterials and promotes early bone healing
Authors: Chen Yun , Li Jia , Shi Jue , Ning Dandan , Feng Jianying , Lin Weiwei , He Fuming , Xie Zhijian 
Published: May 2022

AbstractAimEmerging studies have shown that immune response to biomaterial implants plays a central role in bone healing. Ipriflavone is clinically used for osteoporosis. However, the mechanism of ipriflavone in immune response to implants in early stages of osseointegration remains unclear. In this study, we aimed to investigate the potential role of ipriflavone in early bone healing process and uncover the underlying mechanism.Materials and MethodsWe carried out histological examination as well as analysis of proinflammatory cytokines and NLRP3 inflammasome activation in a tibial implantation mouse model with intra‐peritoneal injection of ipriflavone. In addition, we explored the mechanism of ipriflavone in the regulation of NLRP3 inflammasome activation in macrophages.ResultsIn vivo, ipriflavone ameliorated host inflammatory response related to NLRP3 inflammasome activation at implantation sites, characterized by reductions of inflammatory cell infiltration and proinflammatory cytokine interleukin‐1β levels. Ipriflavone treatment also showed beneficial effects on early osseointegration. Further investigations of the molecular mechanism showed that the suppression of NLRP3 inflammasome acts upstream of NLRP3 oligomerization through abrogating the production of reactive oxygen species.ConclusionsThese results revealed an anti‐inflammatory role of ipriflavone in NLRP3 inflammasome activation through improving mitochondrial function. This study provides a new strategy for the development of immune‐regulated biomaterials and treatment options for NLRP3‐related diseases.
Activation and increased production of interleukin‐17 and tumour necrosis factor‐α of mucosal‐associated invariant T cells in patients with periodontitis
Authors: Kim Ok‐Su , Park Ki‐Jeong , Jin Hye‐Mi , Cho Young‐Nan , Kim Ye Seul , Kwon Seung‐Hee , Koh Jeong‐Tae , Ju Jae Kyun , Kee Seung‐Jung , Park Yong‐Wook 
Published: May 2022

AbstractAimMucosal‐associated invariant T (MAIT) cells are known to be resident in oral mucosal tissue, but their roles in periodontitis are unknown. This study aimed to examine the level and function of MAIT cells in periodontitis patients.Materials and MethodsFrequency, activation, and function of MAIT cells from 28 periodontitis patients and 28 healthy controls (HCs) were measured by flow cytometry.ResultsCirculating MAIT cells were numerically reduced in periodontitis patients. Moreover, they exhibited higher expression of CD69 and annexin V, together with more increased production of interleukin (IL)‐17 and tumour necrosis factor (TNF)‐α, in periodontitis patients than in HCs. Interestingly, periodontitis patients had higher frequencies of MAIT cells in gingival tissue than in peripheral blood. In addition, circulating MAIT cells had elevated expression of tissue‐homing chemokine receptors such as CCR6 and CXCR6, and the corresponding chemokines (i.e., CCL20 and CXCL16) were more strongly expressed in inflamed gingiva than in healthy gingiva.ConclusionsThis study demonstrates that circulating MAIT cells are numerically deficient with an activated profile toward the production of IL‐17 and TNF‐α in periodontitis patients. Furthermore, circulating MAIT cells have the potential to migrate to inflamed gingival tissues.
Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study
Authors: Reckelkamm Stefan Lars , Hannemann Anke , Kocher Thomas , Nauck Matthias , Völzke Henry , Ehmke Benjamin , Rauner Martina , Alayash Zoheir , Baumeister Sebastian‐Edgar , Nolde Michael , Holtfreter Birte 
Published: May 2022

AbstractAimTo examine the associations between bone turnover markers and periodontitis in two cross‐sectional population‐based studies.Materials and MethodsWe used data from two independent adult samples (N = 4993), collected within the Study of Health in Pomerania project, to analyse cross‐sectional associations of N‐procollagen type 1 amino‐terminal propeptide (P1NP), C‐terminal cross‐linking telopeptide, osteocalcin, bone‐specific alkaline phosphatase (BAP), fibroblast growth factor 23, wingless‐type mouse mammary tumour virus integration site family member 5a (WNT5A), and sclerostin values with periodontitis. Confounder‐adjusted gamma and fractional response regression models were applied.ResultsPositive associations were found for P1NP with mean pocket probing depth (PPD; ; 95% confidence interval [CI]: 1.001–1.015), mean clinical attachment loss (mean CAL; ; 95% CI: 1.011–1.044), and proportion of sites with bleeding on probing (%BOP; ; 95% CI: 1.005–1.109). Similar associations were seen for BAP with %BOP (; 95% CI: 1.042–1.205), proportion of sites with PPD ≥4 mm (%PPD4) (; 95% CI: 1.005–1.161), and sclerostin with %BOP (; 95% CI: 1.005–1.704). WNT5A was inversely associated with mean PPD (; 95% CI: 0.920–0.993) and %PPD4 (; 95% CI: 0.642–0.982).ConclusionsThis study revealed scattered associations of P1NP, BAP, WNT5A, and sclerostin with periodontitis, but the results are contradictory in the overall context. Associations reported in previous studies could not be confirmed.
Carvone and citral, two promising compounds for controlling the honey bee ectoparasitic mite, Varroa destructor
Authors: Sabahi Qodratollah , Kelly Paul Gordon , Guzman‐Novoa Ernesto 
Published: May 2022

AbstractVarroa destructor (Acari: Varroidae) is an external parasitic mite that has compromised honey bee (Hymenoptera: Apidae) health worldwide. Varroa mite resistance to commercial formulations of synthetic acaricides has led to an increasing need for new compounds to control mite infestations in honey bee colonies. Thus, essential oils and plant extracts have been used by organic producers concerned about the environment because they are low‐risk pesticides. This study tested the toxicity of four natural compounds: carvone, citral, cineole and limonene to mites and bees under laboratory conditions. Tau‐fluvalinate was used as positive control. Seven concentrations with logarithmic interval were used to test each compound. The safety margin of the compounds for the bees was calculated from selectivity ratios. Carvone was significantly more toxic to varroa mites (LC50 = 272.74 μg/ml) than the other three compounds but did not differ with tau‐fluvalinate (LC50 = 272.30 μg/ml). Citral was the second most toxic compound to mites (LC50 = 318.54 μg/ml), followed by cineole (LC50 = 3897.65 μg/ml) and limonene (LC50 = 3003.94 μg/ml). Citral and carvone showed low toxicity to worker bees at both 24 and 48 hpt. The LD50 values for citral, carvone and tau‐fluvalinate were 78,459, 106,620 and 143.02 μg/ml at 24 hpt, respectively, which were significantly different from each other. The relative selectivity ratios calculated for carvone, citral and tau‐fluvalinate were 390.9, 246.3 and 0.53, respectively. This study provides preliminary laboratory evidence for the effectiveness and safety of two plant compounds, carvone and citral, that could potentially be used for the control of V. destructor infestations in honey bee colonies.
Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening
Authors: Wang Jinfang , Tian Shouwei , Yu Yongtao , Ren Yi , Guo Shaogui , Zhang Jie , Li Maoying , Zhang Haiying , Gong Guoyi , Wang Min , Xu Yong 
Published: May 2022

ABSTRACTThe NAC transcription factor NONRIPENING (NOR) is a master regulator of climacteric fruit ripening. Melon (Cucumis melo L.) has climacteric and non‐climacteric fruit ripening varieties and is an ideal model to study fruit ripening. Two natural CmNAC‐NOR variants, the climacteric haplotype CmNAC‐NORS,N and the non‐climacteric haplotype CmNAC‐NORA,S, have effects on fruit ripening; however, their regulatory mechanisms have not been elucidated. Here, we report that a natural mutation in the transcriptional activation domain of CmNAC‐NORS,N contributes to climacteric melon fruit ripening. CmNAC‐NOR knockout in the climacteric‐type melon cultivar “BYJH” completely inhibited fruit ripening, while ripening was delayed by 5–8 d in heterozygous cmnac‐nor mutant fruits. CmNAC‐NOR directly activated carotenoid, ethylene, and abscisic acid biosynthetic genes to promote fruit coloration and ripening. Furthermore, CmNAC‐NOR mediated the transcription of the “CmNAC‐NOR‐CmNAC73‐CmCWINV2” module to enhance flesh sweetness. The transcriptional activation activity of the climacteric haplotype CmNAC‐NORS,N on these target genes was significantly higher than that of the non‐climacteric haplotype CmNAC‐NORA,S. Moreover, CmNAC‐NORS,N complementation fully rescued the non‐ripening phenotype of the tomato (Solanum lycopersicum) cr‐nor mutant, while CmNAC‐NORA,S did not. Our results provide insight into the molecular mechanism of climacteric and non‐climacteric fruit ripening in melon.
An exploration of nurses' knowledge, attitudes and intentions regarding restraint use in elderly residential care facilities
Authors: Redmond Liam Gerard , Hyland Pauline K. , Orr Patricia Meredith , White Mark 
Published: May 2022

AbstractPurposeIn Ireland, there is a regulatory focus on restraint minimisation in elderly residential care facilities. Consistent with this focus, this study aimed to explore and identify the relationship between nurses' knowledge levels, attitudes and intentions regarding physical restraint use in two large Irish elderly residential care facilities.DesignA correlational and cross‐sectional survey design was used to collect data on variables including nurses' education levels, years of experience and intentions toward restraint utilization.MethodsData was obtained from a sample of 83 nurses in early 2020 via an anonymous, adapted survey measuring knowledge, attitude and intentions.ResultsResults showed high knowledge levels, negative attitudes toward restraint implementation, and moderate mean intention scores. A significant positive relationship existed between knowledge and attitudes, with both variables negatively predicting intentions regarding restraint. Education was significant in predicting knowledge and attitudes; however, years of experience reported no such findings.ConclusionKnowledge and attitudes negatively predict nurses' intentions toward restraint, with attitude being the stronger predictor of intentions. Falls risk caused the greatest variation in intention scores.Clinical RelevanceThis research offered a seminal study providing insight into the use of restraints in an Irish context with findings that are in line with international research. It highlights the importance of knowledge and attitudes along with education with understanding intentions to use restraints. Furthermore this research demonstrates a useful instrument in the assessment of nurses' knowledge, attitudes, and intentions in Irish elderly residential care facilities, which can possibly be used in other settings.
Anatomic update on the 3‐dimensionality of the subdermal septum and its relevance for the pathophysiology of cellulite
Authors: Cotofana Sebastian , Kaminer Michael S. 
Published: May 2022

AbstractBackgroundCellulite is an aesthetic condition affecting the appearance of skin in specific body regions. When reviewing past literature, a 2‐D image of a subdermal septum was created most likely due to the applied cross‐sectional investigative methodology. Despite practitioners being aware of the 3‐D nature of the subdermal architecture, this is not reflected in the present scientific literature. The aim of this anatomic review is to summarize the past literature and to provide an update on the 3‐dimensionality of a subdermal septum with a specific focus on the pathophysiology of cellulite.Materials and MethodsThis review is based on the literature search performed in the PubMed database using the keywords: cellulite (n = 777), cellulite, AND pathophysiology (n = 53). The articles obtained were screened and those focusing on “cellulitis” or other non‐cellulite‐related topics were additionally excluded resulting in a total of n = 38 relevant articles which were evaluated for the purpose of this anatomic review.ResultsThe skin is comprised of two fat layers (superficial and deep), separated by the superficial fascia. The dynamic 3‐D interplay between retinacula cutis, fascia, and fat, with anatomic differences between men and women, highlights a complex anatomic construct with direct implications for the formation and treatment of cellulite.ConclusionThe 3‐dimensionality of a subdermal septum provides important clinical clues to understanding the underlying mechanisms and pathogenesis of cellulite. The 3‐D approach, in contrast to the past 2‐D models, presents a robust foundation for understanding and developing future cellulite therapeutic strategies.
Treatment of keratosis pilaris rubra with 577‐nm pro‐yellow laser
Authors: Temiz Selami Aykut , Ataseven Arzu , Dursun Recep 
Published: May 2022

AbstractObjectiveKeratosis pilaris rubra (KPR) is a rare group of idiopathic hereditary disorders of keratinization, and it is considered as variants of keratosis pilaris. It is characterized by a well‐defined erythema and small, keratotic follicular papules that are seen on the cheeks and preauricular area. Keratosis pilaris rubra is an aesthetically distressed situation, and especially vascular erythema is the most common complaint. In recent years, pro‐yellow (577 nm) laser, laser system with yellow light wavelength, has been used as an alternative for seeking more effective treatment especially in vascular lesions. However, in the literature, pro‐yellow laser therapy has never been used before in keratosis pilaris rubra. Therefore, we wanted to evaluate the effectiveness of the pro‐yellow laser in keratosis pilaris rubra patients.Materials and MethodsIn our study, four patients with keratosis pilaris rubra treated with pro‐yellow laser in our Cosmetology Unit between December 2017 and March 2019 were evaluated. The first session was started with 20 j/cm2 and the dose increased 2 j/cm2 at each session. The dose was increased up to 26 j/cm2, a total of four sessions (20–22–24–26 j/cm2) was applied in treatment. All the sessions were used in treatment scanner mode.ResultsObjectifying a clearance of erythema >75% was clinically evident in three patients, in the fourth patient, erythema regressed approximately 50%. There has been no recurrence of the lesions after a minimum three months follow‐up. There was no permanent side effect in any patient.ConclusionPro‐yellow laser is a well option for the treatment of keratosis pilaris rubra, and we think that it could be a safety choice therapy. In addition, a well tolerance to treatment and a low incidence of serious side effects make it a very reliable therapy. Further clinical studies are needed to improve our findings.
Orthokeratinized odontogenic cysts: A clinicopathologic study of 159 cases and molecular evidence for the absence of PTCH1 mutations
Authors: Wang Yan‐Jin , Zhang Jian‐Yun , Dong Qing , Li Tie‐Jun 
Published: May 2022

AbstractBackgroundOrthokeratinized odontogenic cyst (OOC), a newly designated entity of odontogenic cysts, is an intraosseous jaw cyst that is entirely or predominantly lined by orthokeratinized squamous epithelium. The aim of this study was to report a large series of OOC to substantiate its clinicopathologic profiles and to investigate PTCH1 mutations in OOCs.MethodThe clinicopathologic features of 167 OOCs from 159 patients were analyzed and the immunohistochemical expression of markers related to cell differentiation and proliferation was evaluated. Furthermore, PTCH1 mutations were analyzed in 14 fresh samples of OOC.ResultsOOCs occurred mostly in the third and fourth decades (60.4%) with a male predilection (66.7%). The lesions developed more often in the mandible than maxilla, primarily in the posterior mandible and ramus. Eight patients (5.0%) showed multiple locations of either bilateral posterior mandible (n = 6) or both the maxilla and mandible. Radiographically, the majority of OOCs (91.2%) showed a well‐demarcated, unilocular radiolucency with 14 multilocular cases (8.8%). A follow‐up of 131 patients (123 treated by enucleation with or without marsupialization and eight by peripheral ostectomy) revealed no recurrence during an average period of 4.56 years after surgery. Immunohistochemistry indicated lower proliferative activity and a varying epithelial differentiation pattern in OOC compared with odontogenic keratocysts (OKC). No PTCH1 mutation was detected, except for three known single nucleotide polymorphisms.ConclusionThe clinicopathological and molecular differences between OOC and OKC justified their separation, and unlike OKCs, OOCs did not harbor PTCH1 mutations, suggesting different pathogenesis underlying these two jaw cysts.
Balancing ecosystem integrity and cultural values at sacred Lake Hora, Ethiopia: The need for conservation of wetland vegetation
Authors: Gebrehiwot Mesfin , Gebrekidan Berhanu , Asmare Melese , Kifle Demeke , Adane Metadel , Girma Haileyesus , Triest Ludwig , Stiers Iris 
Published: May 2022

AbstractAlthough macrophytes represent important habitats for the plankton communities of shallow lakes and ponds, the role of littoral macrophyte patches in deep crater lakes is not well described. The objective of the present study was to investigate the role of littoral macrophyte vegetation in structuring phytoplankton and zooplankton communities and, therefore, sustaining ecological integrity in a deep tropical crater lake (Hora Lake, Ethiopia). The lake has a surface area of 1.03 km2 and a mean depth of 16.5 m. The area surrounding the lake is a prime location for the celebration of the Oromo thanksgiving festival (Irreecha), which is inscribed by the United Nations Educational, Scientific and Cultural Organization (UNESCO) as an intangible cultural heritage, and also considered sacred by the Oromo people. Measurements of physical, chemical and biological parameters were conducted at fifteen sampling sites in both the littoral and central parts of the lake during the dry and wet seasons in 2017. A total of 28 phytoplankton and 19 zooplankton genera were identified. The phytoplankton and zooplankton communities were dominated by cyanobacteria and rotifers, respectively, at the open water sites. The wetland vegetation was found to be important in reducing potentially toxic and unsightly cyanobacteria. The submerged and emergent vegetation also served as refugia for zooplankton during the wet and dry seasons, respectively. Crustaceans accounted for about 30–45% of the total zooplankton abundance within the vegetation fringes. Their abundance decreased to 5% in the non‐vegetated areas, wherein the community was dominated by rotifers. As the population around the lake, particularly during the festival, may damage the aquatic vegetation, their access to the shoreline should be regulated for conservation and sanitary reasons (e.g. to reduce cyanobacteria growths).
Effects of the neo‐X chromosome on genomic signatures of hybridization inRumex hastatulus
Authors: Beaudry Felix E. G. , Rifkin Joanna L. , Peake Amanda L. , Kim Deanna , Jarvis‐Cross Madeline , Barrett Spencer C. H. , Wright Stephen I. 
Published: May 2022

AbstractNatural hybrid zones provide opportunities for studies of the evolution of reproductive isolation in wild populations. Although recent investigations have found that the formation of neo‐sex chromosomes is associated with reproductive isolation, the mechanisms remain unclear in most cases. Here, we assess the contemporary structure of gene flow in the contact zone between largely allopatric cytotypes of the dioecious plantRumex hastatulus,a species with evidence of sex chromosome turn‐over. Males to the west of the Mississippi river, USA, have an X and a single Y chromosome, whereas populations to the east of the river have undergone a chromosomal rearrangement giving rise to a larger X and two Y chromosomes. Using reduced‐representation sequencing, we provide evidence that hybrids form readily and survive multiple backcross generations in the field, demonstrating the potential for ongoing gene flow between the cytotypes. Cline analysis of each chromosome separately captured no signals of difference in cline shape between chromosomes. However, principal component regression revealed a significant increase in the contribution of individual SNPs to inter‐cytotype differentiation on the neo‐X chromosome, but no correlation with recombination rate. Cline analysis revealed that the only SNPs with significantly steeper clines than the genome average were located on the neo‐X. Our data are consistent with a role for neo‐sex chromosomes in reproductive isolation betweenR. hastatuluscytotypes. Our investigation highlights the importance of studying plant hybrid zones for understanding the evolution of sex chromosomes.
Is English the Culprit? Longitudinal Associations Between Students’ Value Beliefs in English, German, and French in Multilingual Switzerland
Authors: COSTACHE OANA , BECKER EVA S. , GOETZ THOMAS 
Published: May 2022

AbstractMotivational interactions during multiple language learning have been largely neglected in language motivation research. To fill this gap, we investigate longitudinal relations between Swiss German students’ value beliefs in English, French, and German in upper secondary schools and whether there are differences in motivational development between multilingual and monolingual students. Multivariate latent growth modeling was used to analyze data from 850 students (Mage =  15.61 years, SD =  .62; 54% female) gathered yearly from Grades 9 to 11. Results suggest an interference between students’ value beliefs in English and the other 2 languages. Students who reported higher value beliefs in English in Grade 9 showed steeper decreases in their value beliefs for French and German from Grades 9 to 11. However, stronger increases in English value beliefs over time were associated with stronger increases in French and German value beliefs. Moreover, while multilingual students reported higher initial value beliefs in French and English, they also showed steeper decreases in French and English value beliefs over time compared to their monolingual peers. Findings are discussed in relation to their implications for teaching practice and future research directions.