bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term corrosion monitoring of carbon steels and environmental correlation analysis via the random forest method
Authors: Li Qing , Xia Xiaojian , Pei Zibo , Cheng Xuequn , Zhang Dawei , Xiao Kui , Wu Jun , Li Xiaogang 
Published: January 2022

AbstractIn this work, the atmospheric corrosion of carbon steels was monitored at six different sites (and hence, atmospheric conditions) using Fe/Cu-type atmospheric corrosion monitoring technology over a period of 12 months. After analyzing over 3 million data points, the sensor data were interpretable as the instantaneous corrosion rate, and the atmospheric “corrosivity” for each exposure environment showed highly dynamic changes from the C1 to CX level (according to the ISO 9223 standard). A random forest model was developed to predict the corrosion rate and investigate the impacts of ten “corrosive factors” in dynamic atmospheres. The results reveal rust layer, wind speed, rainfall rate, RH, and chloride concentration, played a significant role in the corrosion process.
High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr−/−.Leiden mouse model
Authors: Komninos D. , Ramos L. , van der Heijden G. W. , Morrison M. C. , Kleemann R. , van Herwaarden A. E. , Kiliaan A. J. , Arnoldussen I. A. C. 
Published: January 2022

AbstractObesity can disturb spermatogenesis and subsequently affect male fertility and reproduction. In our study, we aim to elucidate at which cellular level of adult spermatogenesis the detrimental effects of obesity manifest. We induced high fat diet (HFD) obesity in low-density lipoprotein receptor knock-out Leiden (Ldlr−/−.Leiden) mice, and studied the morphological structure of the testes and histologically examined the proportion of Sertoli cells, spermatocytes and spermatids in the seminiferous tubules. We examined sperm DNA damage and chromatin condensation and measured plasma levels of leptin, testosterone, cholesterol and triglycerides. HFD-induced obesity caused high plasma leptin and abnormal testosterone levels and induced an aberrant intra-tubular organisation (ITO) which is associated with an altered spermatids/spermatocytes ratio (2:1 instead of 3:1). Mice fed a HFD had a higher level of tubules in stages VII + VIII in the spermatogenic cycle. The stages VII + VII indicate crucial processes in spermatogenic development like initiation of meiosis, initiation of spermatid elongation, and release of fully matured spermatids. In conclusion, HFD-induced obese Ldlr−/−.Leiden mice develop an aberrant ITO and alterations in the spermatogenic cycle in crucial stages (stages VII and VII). Thereby, our findings stress the importance of lifestyle guidelines in infertility treatments.
Distinct gut microbiomes in two polar bear subpopulations inhabiting different sea ice ecoregions
Authors: Franz Megan , Whyte Lyle , Atwood Todd C. , Laidre Kristin L. , Roy Denis , Watson Sophie E. , Góngora Esteban , McKinney Melissa A. 
Published: January 2022

AbstractGut microbiomes were analyzed by 16S rRNA gene metabarcoding for polar bears (Ursus maritimus) from the southern Beaufort Sea (SB), where sea ice loss has led to increased use of land-based food resources by bears, and from East Greenland (EG), where persistent sea ice has allowed hunting of ice-associated prey nearly year-round. SB polar bears showed a higher number of total (940 vs. 742) and unique (387 vs. 189) amplicon sequence variants and higher inter-individual variation compared to EG polar bears. Gut microbiome composition differed significantly between the two subpopulations and among sex/age classes, likely driven by diet variation and ontogenetic shifts in the gut microbiome. Dietary tracer analysis using fatty acid signatures for SB polar bears showed that diet explained more intrapopulation variation in gut microbiome composition and diversity than other tested variables, i.e., sex/age class, body condition, and capture year. Substantial differences in the SB gut microbiome relative to EG polar bears, and associations between SB gut microbiome and diet, suggest that the shifting foraging habits of SB polar bears tied to sea ice loss may be altering their gut microbiome, with potential consequences for nutrition and physiology.
A systematic review and meta-analysis of the catastrophic costs incurred by tuberculosis patients
Authors: Ghazy Ramy Mohamed , El Saeh Haider M. , Abdulaziz Shaimaa , Hammouda Esraa Abdellatif , Elzorkany Amira Mohamed , Khidr Heba , Zarif Nardine , Elrewany Ehab , Abd ElHafeez Samar 
Published: January 2022

AbstractOne of the strategies of the World Health Organization End Tuberculosis (TB) was to reduce the catastrophic costs incurred by TB-affected families to 0% by 2020.Catastrophic cost is defined by the total cost related to TB management exceeding 20% of the annual pre-TB household income. This study aimed to estimate the pooled proportion of TB affected households who incurred catastrophic costs. We searched PubMed, SciELO, Scopus, Embase, Google Scholar, ProQuest, SAGE, and Web of Science databases according to Preferred Reporting Items of the Systematic Reviews and Meta-Analysis (PRISMA) guidelines till November 20, 2020. Eligible studies were identified and data on catastrophic costs due to TB were extracted. We performed a meta-analysis to generate the pooled proportion of patients with TB facing catastrophic costs. From 5114 studies identified, 29 articles were included in the final analysis. The pooled proportion of patients faced catastrophic costs was (43%, 95% CI [34–51]). Meta-regression revealed that country, drug sensitivity, and Human immune-deficiency Virus (HIV) co-infection were the main predictors of such costs. Catastrophic costs incurred by drug sensitive, drug resistant, and HIV co-infection were 32%, 81%, and 81%, respectively. The catastrophic costs incurred were lower among active than passive case findings (12% vs. 30%). Half (50%) of TB-affected households faced catastrophic health expenditure at 10% cut-off point. The financial burden of patients seeking TB diagnosis and treatment continues to be a worldwide impediment. Therefore, the End TB approach should rely on socioeconomic support and cost-cutting initiatives.PROSPERO registration: CRD42020221283.
Prone versus supine free-breathing for right-sided whole breast radiotherapy
Authors: Fargier-Bochaton Odile , Wang Xinzhuo , Dipasquale Giovanna , Laouiti Mohamed , Kountouri Melpomeni , Gorobets Olena , Nguyen Nam P. , Miralbell Raymond , Vinh-Hung Vincent 
Published: January 2022

AbstractProne setup has been advocated to improve organ sparing in whole breast radiotherapy without impairing breast coverage. We evaluate the dosimetric advantage of prone setup for the right breast and look for predictors of the gain. Right breast cancer patients treated in 2010–2013 who had a dual supine and prone planning were retrospectively identified. A penalty score was computed from the mean absolute dose deviation to heart, lungs, breasts, and tumor bed for each patient's supine and prone plan. Dosimetric advantage of prone was assessed by the reduction of penalty score from supine to prone. The effect of patients' characteristics on the reduction of penalty was analyzed using robust linear regression. A total of 146 patients with right breast dual plans were identified. Prone compared to supine reduced the penalty score in 119 patients (81.5%). Lung doses were reduced by 70.8%, from 4.8 Gy supine to 1.4 Gy prone. Among patient's characteristics, the only significant predictors were the breast volumes, but no cutoff could identify when prone would be less advantageous than supine. Prone was associated with a dosimetric advantage in most patients. It sets a benchmark of achievable lung dose reduction.Trial registration: ClinicalTrials.gov NCT02237469, HUGProne, September 11, 2014, retrospectively registered.
Analysis of coal face stability of lower coal seam under repeated mining in close coal seams group
Authors: Xiong Yu , Kong Dezhong , Wen Zhijie , Wu Guiyi , Liu Qinzhi 
Published: January 2022

AbstractAiming at the problem of coal face failure of lower coal seam under the influence of repeated mining in close coal seams, with the working face 17,101 as a background, the coal samples mechanics test clarified the strength characteristics of the coal face under repeated mining, through similar simulation experiments, the development of stable roof structure and surrounding rock cracks under repeated mining of close coal seams are further explored. And based on this, establish a coal face failure mechanics model to comprehensively analyze the influence of multiple roof structural instabilities on the stability of the coal face. Finally, numerical simulation is used to further supplement and verify the completeness and rationality of similar simulation experiment and theoretical analysis results. The results show that: affected by repeated mining disturbances, the cracks in the coal face are relatively developed, the strength of the coal body is reduced, and the coal face is more prone to failure under the same roof pressure; During the mining of coal seam 17#, the roofs of different layers above the stope form two kinds of "arch" structures and one kind of “voussoir beam” structure, and there are three different degrees of frequent roof pressure phenomenon, which is easy to cause coal face failure; Under repeated mining of close coal seams, the roof pressure acting on the coal face is not large. The main controlling factor of coal face failure is the strength of the coal body, and the form of coal face failure is mostly the shear failure of soft coal. The research results can provide a theoretical basis for coal face failure under similar conditions.
Active earth pressure against flexible retaining wall for finite soils under the drum deformation mode
Authors: Hu Weidong , Zhu Xinnian , Zeng Yongqing , Liu Xiaohong , Peng Chucai 
Published: January 2022

AbstractA reasonable method is proposed to calculate the active earth pressure of finite soils based on the drum deformation mode of the flexible retaining wall close to the basement’s outer wall. The flexible retaining wall with cohesionless sand is studied, and the ultimate failure angle of finite soils close to the basement’s outer wall is obtained using the Coulomb theory. Soil arch theory is led to get the earth pressure coefficient in the subarea using the trace line of minor principal stress of circular arc after stress deflection. The soil layers at the top and bottom part of the retaining wall are restrained when the drum deformation occurs, and the soil layers are in a non-limit state. The linear relationship between the wall movement’s magnitude and the mobilization of the internal friction angle and the wall friction anger is presented. The level layer analysis method is modified to propose the resultant force of active earth pressure, the action point’s height, and the pressure distribution. Model tests are carried out to emulate the process of drum deformation and soil rupture with limited width. Through image analysis, it is found that the failure angle of soil within the limited width is larger than that of infinite soil. With the increase of the aspect ratio, the failure angle gradually reduces and tends to be constant. Compared with the test results, it is shown that the horizontal earth pressure reduces with the reduction of the aspect ratio within critical width, and the resultant force decreases with the increase of the limit state region under the same ratio. The middle part of the distribution curve is concave. The active earth pressure strength decreases less than Coulomb’s value, the upper and lower soil layers are in the non-limit state, and the active earth pressure strength is more than Coulomb’s value.
Suppression of lipopolysaccharide-induced corneal opacity by hepatocyte growth factor
Authors: Elbasiony Elsayed , Cho WonKyung , Mittal Sharad K. , Chauhan Sunil K. 
Published: January 2022

AbstractKeratitis induced by bacterial toxins, including lipopolysaccharide (LPS), is a major cause of corneal opacity and vision loss. Our previous study demonstrates hepatocyte growth factor (HGF) promotes epithelial wound healing following mechanical corneal injury. Here, we investigated whether HGF has the capacity to suppress infectious inflammatory corneal opacity using a new model of LPS-induced keratitis. Keratitis, induced by two intrastromal injections of LPS on day 1 and 4 in C57BL/6 mice, resulted in significant corneal opacity for up to day 10. Following keratitis induction, corneas were topically treated with 0.1% HGF or PBS thrice daily for 5 days. HGF-treated mice showed a significantly smaller area of corneal opacity compared to PBS-treated mice, thus improving corneal transparency. Moreover, HGF treatment resulted in suppression of α-SMA expression, compared to PBS treatment. HGF-treated corneas showed normalized corneal structure and reduced expression of pro-inflammatory cytokine, demonstrating that HGF restores corneal architecture and immune quiescence in corneas with LPS-induced keratitis. These findings offer novel insight into the potential application of HGF-based therapies for the prevention and treatment of infection-induced corneal opacity.
A novel molecular diagnostic method for the gut content analysis of Philaenus DNA
Authors: Rodrigues Isabel , Ramos Vítor , Benhadi-Marín Jacinto , Moreno Aránzazu , Fereres Alberto , Pereira José Alberto , Baptista Paula 
Published: January 2022

AbstractPhilaenus spumarius is a vector of Xylella fastidiosa, one of the most dangerous plants pathogenic bacteria worldwide. There is currently no control measure against this pathogen. Thus, the development of vector control strategies, like generalist predators, such as spiders, could be essential to limit the spread of this vector-borne pathogen. In this study, a polymerase chain reaction (PCR)-based approach was developed to principally detect DNA of P. spumarius in the spider’s gut. Accordingly, 20 primer pairs, targeting the mitochondrial cytochrome oxidase I (COI) and cytochrome b (cytB) genes, were tested for specificity, sensitivity, and efficiency in detecting P. spumarius DNA. Overall, two primer sets, targeting COI gene (COI_Ph71F/COI_Ph941R) and the cytB gene (cytB_Ph85F/cytB_Ph635R), showed the highest specificity and sensitivity, being able to amplify 870 pb and 550 bp fragments, respectively, with P. spumarius DNA concentrations 100-fold lower than that of the DNA of non-target species. Among these two primer sets, the cytB_Ph85F/cytB_Ph635R was able to detect P. spumarius in the spider Xysticus acerbus, reaching 50% detection success 82 h after feeding. The feasibility of this primer set to detect predation of P. spumarius by spiders was confirmed in the field, where 20% of the collected spiders presented positive amplifications.
The AtMYB60 transcription factor regulates stomatal opening by modulating oxylipin synthesis in guard cells
Authors: Simeoni Fabio , Skirycz Aleksandra , Simoni Laura , Castorina Giulia , de Souza Leonardo Perez , Fernie Alisdair R. , Alseekh Saleh , Giavalisco Patrick , Conti Lucio , Tonelli Chiara , Galbiati Massimo 
Published: January 2022

AbstractStomata are epidermal pores formed by pairs of specialized guard cells, which regulate gas exchanges between the plant and the atmosphere. Modulation of transcription has emerged as an important level of regulation of stomatal activity. The AtMYB60 transcription factor was previously identified as a positive regulator of stomatal opening, although the details of its function remain unknown. Here, we propose a role for AtMYB60 as a negative modulator of oxylipins synthesis in stomata. The atmyb60-1 mutant shows reduced stomatal opening and accumulates increased levels of 12-oxo-phytodienoic acid (12-OPDA), jasmonic acid (JA) and jasmonoyl-l-isoleucine (JA-Ile) in guard cells. We provide evidence that 12-OPDA triggers stomatal closure independently of JA and cooperatively with abscisic acid (ABA) in atmyb60-1. Our study highlights the relevance of oxylipins metabolism in stomatal regulation and indicates AtMYB60 as transcriptional integrator of ABA and oxylipins responses in guard cells.
Interfacial reactions in lithia-based cathodes depending on the binder in the electrode and salt in the electrolyte
Authors: Im Hee Jeong , Park Yong Joon 
Published: January 2022

AbstractLithia (Li2O)-based cathodes, utilizing oxygen redox reactions for obtaining capacity, exhibit higher capacity than commercial cathodes. However, they are highly reactive owing to superoxides formed during charging, and they enable more active parasitic (side) reactions at the cathode/electrolyte and cathode/binder interfaces than conventional cathodes. This causes deterioration of the electrochemical performance limiting commercialization. To address these issues, the binder and salt for electrolyte were replaced in this study to reduce the side reaction of the cells containing lithia-based cathodes. The commercially used polyvinylidene fluoride (PVDF) binder and LiPF6 salt in the electrolyte easily generate such reactions, and the subsequent reaction between PVDF and LiOH (from decomposition of lithia) causes slurry gelation and agglomeration of particles in the electrode. Moreover, the fluoride ions from PVDF promote side reactions, and LiPF6 salt forms POF3 and HF, which cause side reactions owing to hydrolysis in organic solvents containing water. However, the polyacrylonitrile (PAN) binder and LiTFSI salt decrease these side reactions owing to their high stability with lithia-based cathode. Further, thickness of the interfacial layer was reduced, resulting in decreased impedance value of cells containing lithia-based cathodes. Consequently, for the same lithia-based cathodes, available capacity and cyclic performance were increased owing to the effects of PAN binder and LiTFSI salt in the electrolyte.
Structure of alumina glass
Authors: Hashimoto Hideki , Onodera Yohei , Tahara Shuta , Kohara Shinji , Yazawa Koji , Segawa Hiroyo , Murakami Motohiko , Ohara Koji 
Published: January 2022

AbstractThe fabrication of novel oxide glass is a challenging topic in glass science. Alumina (Al2O3) glass cannot be fabricated by a conventional melt–quenching method, since Al2O3 is not a glass former. We found that amorphous Al2O3 synthesized by the electrochemical anodization of aluminum metal shows a glass transition. The neutron diffraction pattern of the glass exhibits an extremely sharp diffraction peak owing to the significantly dense packing of oxygen atoms. Structural modeling based on X-ray/neutron diffraction and NMR data suggests that the average Al–O coordination number is 4.66 and confirms the formation of OAl3 triclusters associated with the large contribution of edge-sharing Al–O polyhedra. The formation of edge-sharing AlO5 and AlO6 polyhedra is completely outside of the corner-sharing tetrahedra motif in Zachariasen’s conventional glass formation concept. We show that the electrochemical anodization method leads to a new path for fabricating novel single-component oxide glasses.
Change in the optic nerve sheath diameter after deflation of a pneumatic tourniquet: a prospective observational study
Authors: Kim Ha-Jung , Kim Yeon Ju , Kim Jiyoung , Kim Hyungtae , Ro Young-Jin , Koh Won Uk 
Published: January 2022

AbstractApplying a pneumatic tourniquet provides surgeons with a bloodless surgical field. However, application of the tourniquet induces various physiological changes. We evaluated the effect of tourniquet deflation on the intracranial pressure by using ultrasonography to measure the optic nerve sheath diameter (ONSD) in patients undergoing lower limb surgery. The ONSD was measured in 20 patients at five time points: after anesthetic induction (T0) and immediately before (T1), immediately after (T2), 5 min after (T3), and 10 min after tourniquet deflation (T4). Hemodynamic and respiratory variables were recorded. The ONSD showed significant differences at each point (P < 0.001). The ONSDs at T2 and T3 were significantly greater than that at T1 (P = 0.0007 and < 0.0001, respectively). The change in the end-tidal carbon dioxide partial pressure (EtCO2) was similar to the change in the ONSD. The change in the ONSD was significantly correlated with the change in the EtCO2 after tourniquet deflation (r = 0.484, P = 0.030). In conclusion, the ONSD, as an indicator of intracranial pressure, increased after tourniquet deflation in patients undergoing lower limb surgery. This was correlated with an increased EtCO2 and arterial carbon dioxide partial pressure.Trial registration: ClinicalTrials.gov (NCT03782077).
Automated prediction of the clinical impact of structural copy number variations
Authors: Gažiová M. , Sládeček T. , Pös O. , Števko M. , Krampl W. , Pös Z. , Hekel R. , Hlavačka M. , Kucharík M. , Radvánszky J. , Budiš J. , Szemes T. 
Published: January 2022

AbstractCopy number variants (CNVs) play an important role in many biological processes, including the development of genetic diseases, making them attractive targets for genetic analyses. The interpretation of the effect of these structural variants is a challenging problem due to highly variable numbers of gene, regulatory, or other genomic elements affected by the CNV. This led to the demand for the interpretation tools that would relieve researchers, laboratory diagnosticians, genetic counselors, and clinical geneticists from the laborious process of annotation and classification of CNVs. We designed and validated a prediction method (ISV; Interpretation of Structural Variants) that is based on boosted trees which takes into account annotations of CNVs from several publicly available databases. The presented approach achieved more than 98% prediction accuracy on both copy number loss and copy number gain variants while also allowing CNVs being assigned “uncertain” significance in predictions. We believe that ISV’s prediction capability and explainability have a great potential to guide users to more precise interpretations and classifications of CNVs.
Mechanisms of dispersal and colonisation in a wind-borne cereal pest, the haplodiploid wheat curl mite
Authors: Laska Alicja , Przychodzka Anna , Puchalska Ewa , Lewandowski Mariusz , Karpicka-Ignatowska Kamila , Skoracka Anna 
Published: January 2022

AbstractDispersal and colonisation determine the survival and success of organisms, and influence the structure and dynamics of communities and ecosystems in space and time. Both affect the gene flow between populations, ensuring sufficient level of genetic variation and improving adaptation abilities. In haplodiploids, such as Aceria tosichella (wheat curl mite, WCM), a population may be founded even by a single unfertilised female, so there is a risk of heterozygosity loss (i.e. founder effect). It may lead to adverse outcomes, such as inbreeding depression. Yet, the strength of the founder effect partly depends on the genetic variation of the parental population. WCM is an economically important pest with a great invasive potential, but its dispersal and colonisation mechanisms were poorly studied before. Therefore, here we assessed WCM dispersal and colonisation potential in relation to the genetic variation of the parental population. We checked whether this potential may be linked to specific pre-dispersal actions (e.g. mating before dispersal and collective behaviour). Our study confirms that dispersal strategies of WCM are not dependent on heterozygosity in the parental population, and the efficient dispersal of this species depends on collective movement of fertilised females.