bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiological and biochemical contrasting responses associated with growth performances in sunflower seedlings after a cold stress
Authors: Fabio Ernesta , TOMMASINO EXEQUIEL ARTURO , Grieu Philippe 
Published: December 2022

Abstract
In the climate change context, extreme events have become more frequents. Spring is the sunflower sowing season. Late frosts events or several days of low temperatures often occur causing delays in crop growth and even losses in plant density per hectare. Tolerance to low temperatures is an important trait considering that the sunflower production area is expanding to marginal regions with suboptimal growing conditions. Furthermore, an early sowing practice to maximize the sunflower growing period is commonly implemented in Argentina and in others countries. An interesting approach to identifying tolerant genotypes in breeding programs is exploring biochemical and physiological traits involved in growth recovery after cold exposure in sunflower seedlings. In this research, the physiological and biochemical responses of two contrasting sunflower genotypes were evaluated. Commercial hybrids Pampero (PM) and Sierra (SA) were exposed to cold treatment at 5°C for 96 hours. Growth performance, malondialdehyde content, superoxide dismutase and catalase enzyme activities, chlorophyll content and electrolyte leakage were assessed at 0, 24, 48 and 72 hours after the cold treatment. Different patterns between genotypes were observed. The sensitive genotype PM showed higher oxidative damage, lower membrane stability, reduced catalase and reduced superoxide dismutase activities, in contrast with the tolerant genotype. Moreover, tolerant genotype SA showed a great capacity to recover the chlorophyll content and restart growth while the sensitive genotype showed only a slight increase in chlorophyll content and a clear delay in growth.
Treating tibial fracture with supra-cutaneous locking plate osteosynthesis: personalized therapy for special patient;A case series study
Authors: Zhang Chao , Zhang Junyu , Li Hongjian 
Published: December 2022

Abstract
Background The choice of surgical options for open tibial fractures and closed tibial fractures with soft tissue problems has been controversial.Supra-cutaneous locking plate fixation may be able to provide an personalized treatment plan.
Methods After retrospective analysis of the clinical database, we collected data on 19 patients identified as having chronic osteomyelitis after routine surgery for tibial fractures or open tibial fractures.All patients who underwent external fixation with a supra-cutaneous locking plate were followed up for 12.89±3.81 months postoperatively.Further statistical analysis was not performed because the number of cases was too small and the injuries and fracture types were different.
Results The postoperative Fugl-Meyer lower limb motor function score was 32.47±3.34 in 19 operated patients, which was evaluated according to the Mazur ankle scoring system: 11 cases were excellent, 5 cases were good, 2 cases were acceptable, and 1 case was poor, with an excellent rate of 84%. The duration of time that passed between the implantation of the supra-cutaneous locking plate and its subsequent removal ranged from 13.00±1.05 months.
Conclusions In certain tibial patients with specific indications, such as open tibial fractures without significant bone or soft tissue defect, closed tibial fractures with severe blisters or compromised soft tissue condition, and surgical site infections following tibial internal fixation with no significant bone or soft tissue defect, supra-cutaneous locking plate fixation may be used. It offers sufficient biomechanical stability for fracture repair while minimizing the inconveniences associated with bulky circular frames or external fixators.The supra-cutaneous locking plate fixation used as the final treatment may also result in a little shorter hospital stay for these individuals and lower healthcare expenses.
Sustainable project portfolio selection under uncertainty with consider conflicting projects
Authors: Ramedani Amir Mohammad , Mehrabian Ahmad , Didehkhani Hosein 
Published: December 2022

Abstract
Given the complexity of selecting an industrial project portfolio, portfolio project selection (PPS) has been one of the most crucial concerns of project managers. On the other hand, comprehensive evaluation of projects based on multiple criteria and uncertainty causes the complexity of PPS. Therefore, to improve this problem, this study presents PPS considering a synergy of industrial projects with different criteria related to sustainability, time-dependent, resources, budget, and human resource capability in a two-stage decision under uncertainty. In the first stage, according to the review of historical documents, analysis of checklists, consultants, and specialists the criteria are identified. Then, with a multi-criteria decision-making (MCDM) method, it is considered for scoring with Fuzzy Best-Worst Method (FBWM) and ranking with Fuzzy Vlsekriterijumska Optimizacija IKompromisno Resenje (FVIKOR) method and calculating the sustainable utility of projects. In the second stage, a multi-objective fuzzy-robust-stochastic (FRS) optimization model by maximizing the total profit of the project is a value of the sustainable utilization of the project and minimizing the amount of interruption in project implementation. This model has two challenges including multi-objective and complex, thus to solve the mixed-integer linear programming model, a novel hybrid method according to the Multi-Choice Goal Programming with Utility Function (MCGP-UF) with the Genetics Algorithm (GA) is developed. The outcomes of sensitivity analysis by implementing the model in the real world display that the model and approach presented in varies problems have high flexibility.
Applying multi-objective particle swarm optimization-based dynamic adaptive hyperlink evaluation to focused crawler for meteorological disasters
Authors: Liu Jingfa , Yang Zhihe 
Published: December 2022

Abstract
In traditional semantic-based focused crawlers, the topical priority of unvisited hyperlinks is calculated by linearly integrating pre-defined topical similarity evaluation metrics and their corresponding weighted factors. However, these weighted factors are manually determined by personal experience, which may introduce bias in evaluating unvisited hyperlinks, resulting in topic deviation during crawling. To address this problem, we propose a dynamic adaptive focused crawler, denoted by FCMOPSO, based on multi-objective particle swarm optimization (MOPSO). For topic representation, two domain ontologies of meteorological disasters are constructed. Additionally, we present a comprehensive priority evaluation method (CPEM) of hyperlink concerning both webpage content and hyperlink structure. In MOPSO, the weights of metrics of the CPEM can be updated in every crawling iteration. Furthermore, we utilize the non-dominant sorting with the nearest farthest candidate solution (NS_NFCS) to ensure the diversity of crawling hyperlinks and expand the search range. Compared with focused crawler strategies in the literature, the experimental results on domains of rainstorm disaster and typhoon disaster show that our proposed FCMOPSO achieves satisfactory performance that it can obtain more topic-relevant webpages with ideal time consumption.
Early Identification Methods for Emerging Technologies Based on Weak Signals
Authors: Zhan Chuan , du ye 
Published: December 2022

Abstract

To intelligently identify emerging technology research directions at an early stage, this study proposes a method for the early identification of emerging technology directions on the basis of weak signals and explores the effectiveness of the method. With signal combination map theory, the weak signal keywords of emerging technologies are extracted by text mining. An unsupervised learning model (LDA) is used for topic clustering to further improve the signal interpretation ability and identify weak signal topics. A deep learning model (BERT) is utilized to extend the weak signal topics and make the topic directions clear. By applying the method to augmented reality technology and basing on the data of invention patents and scientific studies in this field in the last decade, we identify six research topics with weak signal characteristics in current augmented reality research; these six are architecture, industrial manufacturing and maintenance, medical care, education, and augmented reality contact lenses, which may be the development directions of future augmented reality research. The weak signal topics are then further expanded to clearly show the specific content of these topics in terms of function, domain, and technology while proving that the early identification method is effective and feasible.
Loss of actin dynamics leads to VDAC dependent MAPK signalling and altered lipid homeostasis that promotes cell death in yeast cells
Authors: Davis Jack , Meyer Thorsten , Smethurst Daniel , Neuhaus Lisa , Heyden Jonas , Broeskamp Filomena , Edrich Elizabeth , Knittelfelder Oskar , Smolnig Martin , Kolb Dagmar , Haar Tobias von der , Gourlay Campbell , Rockenfeller Patrick 
Published: December 2022

Abstract
The dynamic nature of the actin cytoskeleton controls many cellular processes and a loss of its plasticity has been linked to accelerated cell ageing. Cofilin is an actin-binding protein that controls actin dynamics and is linked to mitochondrial signalling pathways that control drug resistance and cell death. Here we show that cofilin driven stabilisation of actin dynamics leads to a loss in cell wall integrity, vacuole fragmentation and disruption of lipid homeostasis, lipid droplet accumulation and the promotion of necrosis. Our data suggest that these phenotypes are triggered by the inappropriate activation of Protein Kinase C (PKC) which leads to constitutive MAPK signalling. Cofilin-driven activation of the MAPK Slt2 requires the presence of the voltage dependent anion channel (VDAC), which resides in the mitochondrial outer membrane, referred to as Porin 1 (Por1) in yeast. This provides further evidence for a link between actin regulation and mitochondrial signalling. Porin has recently been implicated in lipid transport and we find that prevention of excessive lipid droplet accumulation, achieved by deleting the LRO1 and DGA1 genes, was sufficient to prevent Slt2 activation and restore cellular homeostasis in actin stabilised cells. These data suggest that the integrity of the actin cytoskeleton is essential to maintain the fidelity of MAPK signalling and that this in turn is crucial for the maintenance of lipid homeostasis and cell health in S. cerevisiae. Our data also suggest that chronic actin stabilisation leads to mitochondrial VDAC-dependent Slt2 activation and altered, pro-death, cell fate in yeast cells.
Evaluation of Red-Complex Bacteria Loads in Complete Denture Patients
Authors: Veseli Enis , Staka Gloria , Tovani-Palone Marcos Roberto 
Published: December 2022

Abstract
Objective
To evaluate red-complex bacteria (RCB) loads in edentulous patients, before and after dentures’ insertion.
Materials and Methods
Thirty patients were included in the study. Deoxyribonucleic acid (DNA) isolated from bacterial samples were obtained from the dorsum of the tongue before and 3 months after complete dentures (CDs) insertion in order to identify the presence of RCB (Tannerella forsythia, Porphyromonas gingivalis, and Treponema denticola) and quantify their loads, using real-time polymerase chain reaction (RT-PCR). Bacterial loads were represented as “Lg (genome equivalents/sample)” and the data classified according to the “ParodontoScreen” test.
Results
Significant changes in bacterial loads were observed before and 3 months after the CDs insertion for Porphyromonas gingivalis (0.40 ±0.90 vs 1.29 ±1.64, p = 0.0007), Tannerella forsythia (0.36 ±0.94 vs 0.87 ±1.45, p = 0.005), and Treponema denticola (0.11 ±0.41 vs 0.33 ±0.75, p = 0.03). Before the CDs insertion, all patients had a normal bacterial prevalence range (100%) for all analyzed bacteria. Three months after the insertion, 2 (6.7%) of them had a moderate bacterial prevalence range for Porphyromonas gingivalis, while 28 (93.3%) had a normal bacterial prevalence range.
Conclusion
The use of CDs has a significant impact on increasing RCB loads in edentulous patients.
Do Dysfunctional Attitudes Mediate or Moderate the Association Between Everyday Discrimination and Symptoms of Depression in Adolescents?
Authors: Pancost Sherry , Pössel Patrick 
Published: December 2022

Abstract

We aimed to integrate perceived everyday discrimination into Beck’s cognitive model of depression. Participants included 339 adolescents (M = 14.19, 38.9% female), their majority identified as Black or White. Adolescents completed self-report instruments measuring perceived everyday discrimination, dysfunctional attitudes, and depressive symptoms. Our findings indicated a significant interaction effect between perceived everyday discrimination and dysfunctional attitudes on depressive symptoms. The analysis indicated no mediation effect of dysfunctional attitudes as the indirect effect was nonsignificant. If replicated, our findings highlight the importance of clinicians promoting social change to reduce discrimination and them supporting their clients by validating the unfairness of discrimination, help them to mitigate the negative effects of discrimination, and prepare them to cope with the potential of future discrimination.
Analysis and Validation of Antineutrophil cytoplasmic antibody-associated vasculitis with renal injury of the ferroptosis-related gene CD44 and Pan-Cancer
Authors: Tao Mi , Zeng Ziyue , He Yiqing , Li Yuyan , Liao Wenwen , Nie Haihang , Gao Ping 
Published: December 2022

Abstract
Objective: To investigate the role of ferroptosis in Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) with renal injury.
Methods: GSE104954 and GSE108112 were retrieved from the GEO database and concatenated into one dataset. Expression of ferroptosis-related genes (FRGs) was extracted for differential analysis. The ferroptosis signature genes were identified by LASSO regression and SVM-RFE, and their differential expression levels and diagnostic efficacy were verified by independent data sets. The ceRNA (miRNA-TF-mRNA) regulatory network and clinical diagnostic model were constructed respectively. By using consensus clustering, ferroptosis subtypes were identified. ssGSEA and GSVA were employed to assess immune response and pathway activation. Pan-cancer genes were found in TCGA and GTEx. Differential expression of CD44 in was validated by qPCR and immunohistochemistry from HPA database.
Results:Twenty-four FRGs were differentially expressed in patients with AAV kidney injury. Furthermore, five ferroptosis signature genes were identified by two machine learning algorithms. Not only were differentially expressed in independent datasets, the clinical diagnostic model constructed by these genes provided reference for clinical decision-making, but also the ceRNA network revealed their complex regulatory mechanisms. Unsupervised clustering analysis discovered two ferroptosis subtypes with distinct gene expression, immunological microenvironment, and biological functioning pathways. Notably, CD44 was found to be closely associated with many immune cells, most immune responses, and HLA genes, as well as prognosis, immune cell infiltration, TMB, and MSI in patients with a variety of tumors, suggesting it may be a potential intervention target for human diseases including AAV renal injury and tumors.
Conclusions:Ferroptosis in AAV with renal injury is significantly correlated with the immunological microenvironment. For AAV with renal injury and tumors, CD44 could be a useful intervention target.
“Intravitreal fluocinolone acetonide implant: a prospective, pilot study on patients with chronic diabetic macular edema”
Authors: Nicolò Massimo , Desideri Lorenzo Ferro , Rosa Raffaella , Vagge Aldo , Musetti Donatella , Critelli Alice , Corazza Paolo , Traverso Carlo Enrico 
Published: December 2022

Abstract
OBJECTIVES: To analyze the clinical efficacy and safety profile of intravitreal 0.19 mg fluocinolone acetonide (FAc) implant (Iluvien®) in patients with chronic/ refractory diabetic macular edema (DME)
SUBJECTS/METHODS: This a prospective, cross-sectional pilot study enrolling patients with chronic DME, previously treated with intravitreal anti-vascular endothelial growth factors (VEGF) agents and/or intravitreal corticosteroids. The primary endpoints were the modifications in best-corrected visual acuity (BCVA) and central macular thickness (CMT) during the follow-up. Secondary endpoints were recurrence rate, the presence of systemic and/or ocular adverse events (AEs), including changes in intraocular pressure (IOP).
RESULTS: Fourteen eyes of 11 patients were enrolled in this study. Average follow-up period was 18.7 months ± 8.2. Baseline BCVA improved from 59.6 letters to 61.1 letters after 1 month (p=0.994), 63.1 letters after 3 months (p=0.819), 63.7 letters after 6 months (p=0.635), 68.7 letters after 12 months (p=0.07) and 67.7 letters after 18 months (p=0.505). Baseline CMT significantly decreased from 378 µm before Fac treatment to 336 µm after 1 month (p= 0.034), 320 µm after 3 months (p= 0.015), 329 µm after 6 months (p= 0.05), 314 µm after 12 months (p= 0.0025) and 271 µm after 18 months (p= 0.001). Eight eyes (57.1%) needed to be re-injected with anti-VEGF drugs within 12 months and only one (7.1%) was retreated with DEX implant.
No systemic and/ocular AES were reported, including significant variations in IOP.
CONCLUSIONS: FAc implant can be considered an effective and safe treatment option in patients with chronic/refractory DME.
Cluster Analysis Applied to the Spatiotemporal and Climatological Variability of Precipitation over China
Authors: Fernandes Ronabson Cardoso , Porangaba Gislene Figueiredo Ortiz 
Published: December 2022

AbstractRainfall is one of the important climate variables, as it directly affects agriculture, livestock, electricity generation, supplies. And, with the objective of performing a cluster analysis, define homogeneous regions of precipitation for China. For this, the TRMM time series from 1998 to 2017, monthly frequency, was used. With this, a space-time cluster analysis was carried out for China. For verification and validation of these clusters, a linear regression of the homogeneous regions was elaborated with the help of multiple linear regression and its level of significance. The results showed that 8 clusters were needed, ranging from 7.2% (cluster 5) to 18.4% (cluster 3) of China's territory. Although 70.96% of the China region obtained R² greater than 0.99, and 94.88% of its area with R² greater than 0.9, with a valid significance level for this region. Therefore, this work shows an application of cluster analysis with the aid of TRMM data, corroborating to improve the climate understanding of the region.
Retinal microvasculature changes in optical coherence tomography – angiography after severe blunt ocular trauma
Authors: Rocher Antonin , Chirpaz Nicolas , Esminezhad Cedric , Bouvarel Hugo , Burillon Carole , Rochepeau Cédric 
Published: December 2022

Abstract

Background: The pathophysiological origin of the retinal damage present in ocular contusion is not clearly established. It is not known whether it is initially neurodegeneration or vascular ischaemia that leads to retinal atrophy.
Methods: The aim of this study was to evaluate the retinal microvasculature with optical coherence tomography (OCT) - angiography of the eyes affected by severe ocular contusion as compared to the contralateral non-traumatised eyes. The retinal vessel density of superficial (SVP) and deep vascular plexus (DVP), area of choriocapillaris flow voids and foveal avascular zone area were evaluated. The macular thickness in OCT in the acute and late stages of the trauma was also analysed.
Results: A total of 48 patients were included and at both 72 hours and 55 days after severe ocular contusion, there were no significant differences between traumatised and non-traumatised eyes for the vascular density of the SVP, the DVP, the choriocapillaris and area of the foveal avascular zone. But we observed significant increase in macular thickness in traumatised eyes compared to non-traumatised eyes at the initial emergency visit and a decrease in traumatised eyes at follow-up examination.
Conclusions: These results suggest that the micro-vascularisation of the retina and the choriocapillaris are not initially affected in severe ocular contusions. The damage to the retina after trauma is therefore probably neurodegenerative rather than vascular.
Case Report: Biventricular hypertrophic obstructive cardiomyopathy in Patient with Noonan syndrome
Authors: Alfraidy Khaled , Eldeib Moustafa , Amin Mohamed , Nagib Ayman 
Published: December 2022

Abstract
Background: Noonan Syndrome (NS) is characterized by short stature, typical facial dysmorphology, and congenital heart defects. The incidence of NS is estimated to be between 1:1000 and 1:2500 live births. The syndrome is transmitted as an autosomal dominant trait. In approximately 50% of cases, the disease is caused by missense mutations in the PTPN11 gene on chromosome 12, resulting in a gain of function of the non-receptor protein tyrosine phosphatase SHP-2 protein
Case summary: We reported 17-year-old male patient known case of Noonan syndrome with a mutation in PTPN11 gene with HOCM, started to follow up at pediatric cardiology due to murmur, diagnosed as non-obstructive HCM 13 years ago, A year ago he started to follow up at adult cardiology and found to have biventricular obstructive cardiomyopathy.
Oral Administration Sericin-Curcumin Nanoconjugates Inhibits Inflammation and Epithelial-Mesenchymal Transition to Treat Renal Interstitial Fibrosis
Authors: Hu Yang , Zhu Tangsong , gao Shaohui , Duan Aiping , Qin Weisong , Liu Zhihong , Hua Ruinian , Zhang Qiuhong , Bao Hao 
Published: December 2022

Abstract
Background: Chronic kidney disease (CKD) is the most common renal disease. Renal interstitial fibrosis (RIF) is an important marker of the progression of chronic kidney disease. Epithelial-interstitial transformation (EMT) and the release of inflammatory factors are the core mechanisms underlying RIF.
Results: A type of orally administered nanoconjugate composed of curcumin and sericin was successfully synthesized. Following unilateral ureteral obstruction (UUO) in mice, oral gavage of nanoconjugates could significantly downregulate cytokines and proteins that participate in fibrosis in vivo.
Conclusion: In general, sericin-curcumin nanoconjugate dosing via oral administration is an easy and effective therapeutic strategy for suppressing or even reversing the progression of renal interstitial fibrosis.