bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanoscale regulation of Ca2+ dependent phase transitions and real-time dynamics of SAP97/hDLG
Authors: Rajeev Premchand , Singh Nivedita , Kechkar Adel , Butler Corey , Ramanan Narendrakumar , Sibarita Jean-Baptiste , Jose Mini , Nair Deepak 
Published: July 2022

AbstractSynapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. We confirm that the molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Furthermore, the nanoscale molecular signatures of these signalling islands and phase transitions are altered in response to changes in cytosolic Ca2+. Additionally, exchange kinetics of alternatively spliced isoforms of the intrinsically disordered region in SAP97/hDLG C-terminus shows differential sensitivities to Ca2+ bound Calmodulin, affirming that the molecular signatures of local phase transitions of SAP97/hDLG depends on their nanoscale heterogeneity and compositionality of isoforms.
Synthetic periphyton as a model system to understand species dynamics in complex microbial freshwater communities
Authors: Lamprecht Olga , Wagner Bettina , Derlon Nicolas , Tlili Ahmed 
Published: July 2022

AbstractPhototrophic biofilms, also known as periphyton, are microbial freshwater communities that drive crucial ecological processes in streams and lakes. Gaining a deep mechanistic understanding of the biological processes occurring in natural periphyton remains challenging due to the high complexity and variability of such communities. To address this challenge, we rationally developed a workflow to construct a synthetic community by co-culturing 26 phototrophic species (i.e., diatoms, green algae, and cyanobacteria) that were inoculated in a successional sequence to create a periphytic biofilm on glass slides. We show that this community is diverse, stable, and highly reproducible in terms of microbial composition, function, and 3D spatial structure of the biofilm. We also demonstrate the ability to monitor microbial dynamics at the single species level during periphyton development and how their abundances are impacted by stressors such as increased temperature and a herbicide, singly and in combination. Overall, such a synthetic periphyton, grown under controlled conditions, can be used as a model system for theory testing through targeted manipulation.
Epigenetic clocks in relapse after a first episode of schizophrenia
Authors: Segura Àlex-González , Prohens Llucia , Mezquida Gisela , Amoretti Silvia , Bioque Miquel , Ribeiro María , Gurriarán-Bas Xaquin , Rementería Lide , Berge Daniel , Rodriguez-Jimenez Roberto , Roldán Alexandra , Pomarol-Clotet Edith , Ibáñez Angela , Usall Judith , García-Portilla Maria Paz , Cuesta Manuel J. , Parellada Mara , González-Pinto Ana , Berrocoso Esther , Bernardo Miquel , Mas Sergi ,   2EPS group, González-Díaz Jairo M. , Arbelo Néstor , González-Peñas Javier , Pina-Camacho Laura , Diestre Alba , Selma Judit , Zorrilla Iñaki , López Purificación , Trabsa Amira , Monserrat Clara , Sanchez-Pastor Luis , Nuñez-Doyle Aggie , Fatjó-Vilas Mar , Sarró Salvador , Butjosa Anna , Pardo Marta , López-Ilundain Jose M. , Sánchez Torres Ana M. , Saiz-Ruiz Jerónimo , Ochoa-Mangado Enriqueta , RIevero Olga , De-la-Cámara Concepción , Echevarría Rafael Segarra , González-Blanco Leticia 
Published: July 2022

AbstractThe main objective of the present study was to investigate the association between several epigenetic clocks, covering different aspects of aging, with schizophrenia relapse evaluated over a 3-year follow-up period in a cohort of ninety-one first-episode schizophrenia patients. Genome-wide DNA methylation was profiled and four epigenetic clocks, including epigenetic clocks of chronological age, mortality and telomere length were calculated. Patients that relapsed during the follow-up showed epigenetic acceleration of the telomere length clock (p = 0.030). Shorter telomere length was associated with cognitive performance (working memory, r = 0.31 p = 0.015; verbal fluency, r = 0.28 p = 0.028), but no direct effect of cognitive function or symptom severity on relapse was detected. The results of the present study suggest that epigenetic age acceleration could be involved in the clinical course of schizophrenia and could be a useful marker of relapse when measured in remission stages.
Cinnamon extract improves abnormalities in glucose tolerance by decreasing Acyl-CoA synthetase long-chain family 1 expression in adipocytes
Authors: Nishikai-Shen Tsubame , Hosono-Fukao Tomomi , Ariga Toyohiko , Hosono Takashi , Seki Taiichiro 
Published: July 2022

AbstractWe previously demonstrated that cinnamon extract (CE) alleviates streptozotocin-induced type 1 diabetes in rats. The present study aimed to elucidate the detailed molecular target of cinnamon in cultured adipocytes and epididymal adipose tissue of type 2 diabetes model mice. Two-dimensional gel electrophoresis was employed to determine the molecular target of cinnamon in adipocytes. The function of Acyl-CoA synthetase long-chain family-1 (ACSL1), a molecular target of cinnamon that was identified in this study, was further investigated in 3T3-L1 adipocytes using specific inhibitors. Type 2 diabetes model mice (KK-Ay/TaJcl) were used to investigate the effect of CE on glucose tolerance, ACSL1 expression, and related signal molecules in vivo. CE decreased ACSL1 mRNA and protein expression in 3T3-L1 adipocytes but increased glucose uptake and AMPK signaling activation; moreover, a similar effect was observed with an ACSL1 inhibitor. CE improved glucose tolerance and downregulated ACSL1 in mice adipose tissue in vivo. ACSL1 was demonstrated as a molecular target of CE in type 2 diabetes both in a cell culture system and diabetic mouse model.
A new L-shaped rigid beam FBG acceleration sensor
Authors: Teng Yuntian , Wang Yewei , Tang Yixiang , Wang Xiaomei , Li Caihua , Qiu Zhongchao 
Published: July 2022

AbstractAcceleration detection is an important technology in the fields of seismic monitoring, structural health monitoring and resource exploration. A FBG acceleration sensor with the combination of L-shaped rigid beam and spring structure based on bearings is proposed against the low sensitivity that predominates in the low-frequency vibration measurement by FBG acceleration sensors, where L-shaped rigid beam is utilized to amplify the vibration signal, and is fixed by the bearings at both ends to effectively suppress the transverse crosstalk. The effects of structural parameters on the sensitivity and natural frequency of the sensors were analyzed using Origin theory, and such parameters were optimized; next, static stress and modal simulation analysis was made using COMSOL; in the end, a test system was built to test the performance of the real sensors. According to the findings, the acceleration sensor, whose natural frequency is 57 Hz, is of a flat sensitivity response in the low frequency range of 1–35 Hz, with the dynamic range being 89.83 dB, the acceleration sensitivity being up to 1241.85 pm/g, the coefficient of determination R2for the sensitivity fit is 0.9997, and the transverse crosstalk being -26.20 dB within the operating frequency band. The findings offer a reference for improving the low-frequency vibration measurement capability of FBG acceleration sensors.
Anti-cardiolipin IgG autoantibodies associate with circulating extracellular DNA in severe COVID-19
Authors: Bertin Daniel , Brodovitch Alexandre , Lopez Alexandre , Arcani Robin , Thomas Grace M. , Beziane Abdou , Weber Samuel , Babacci Benjamin , Heim Xavier , Rey Louise , Leone Marc , Mege Jean Louis , Bardin Nathalie 
Published: July 2022

AbstractWhereas the detection of antiphospholipid autoantibodies (aPL) in COVID-19 is of increasing interest, their role is still unclear. We analyzed a large aPL panel in 157 patients with COVID-19 according to the disease severity. We also investigated a potential association between aPL and extracellular DNA (exDNA, n = 85) or circulating markers of neutrophil extracellular traps (NET) such as citrullinated histones H3 (CitH3, n = 49). A total of 157 sera of patients infected by SARS-CoV-2 were collected. A large aPL panel including lupus anticoagulant, anti-cardiolipin and anti-beta-2 glycoprotein I (IgG, IgM and IgA), anti-phosphatidylethanolamine IgA, anti-prothrombin (IgG and IgM) was retrospectively analyzed according to the disease severity. We found a total aPL prevalence of 54.8% with almost half of the cases having aCL IgG. Within an extended panel of aPL, only aCL IgG were associated with COVID-19 severity. Additionally, severe patients displayed higher CitH3 levels than mild patients. Interestingly, we highlighted a significant association between the levels of aCL IgG and exDNA only in aCL positive patients with severe disease. In conclusion, we showed a significant link between aPL, namely aCL IgG, and circulating exDNA in patients with severe form of COVID-19, that could exacerbate the thrombo-inflammatory state related to disease severity.
Molecular docking analysis and evaluation of the antimicrobial properties of the constituents of Geranium wallichianum D. Don ex Sweet from Kashmir Himalaya
Authors: Mir Wajahat Rashid , Bhat Basharat Ahmad , Rather Muzafar Ahmad , Muzamil Showkeen , Almilaibary Abdullah , Alkhanani Mustfa , Mir Manzoor Ahmad 
Published: July 2022

AbstractGeranium wallichianumD. Don ex Sweet is a well-known medicinal plant in Kashmir Himalya. The evidence for its modern medicinal applications remains majorly unexplored. The present study was undertaken to elucidate the detailed antimicrobial promises of different crude extracts (methanolic, ethanolic, petroleum ether, and ethyl acetate) ofG. wallichainumagainst common human bacterial and fungal pathogens in order to scientifically validate its traditional use. The LC–MS analysis ofG. wallichainumyielded 141 bioactive compounds with the vast majority of them having therapeutic applications. Determination of minimum inhibitory concentrations (MICs) by broth microdilution method ofG. wallichainumwas tested against bacterial and fungal pathogens with MICs ranging from 0.39 to 400 µg/mL. Furthermore, virtual ligands screening yielded elatine, kaempferol, and germacrene-A as medicinally most active constituents and the potential inhibitors of penicillin-binding protein (PBP), dihydropteroate synthase (DHPS), elongation factor-Tu (Eu-Tu), ABC transporter, 1,3 beta glycan, and beta-tubulin. The root mean square deviation (RMSD) graphs obtained through the molecular dynamic simulations (MDS) indicated the true bonding interactions which were further validated using root mean square fluctuation (RMSF) graphs which provided a better understanding of the amino acids present in the proteins responsible for the molecular motions and fluctuations. The effective binding of elatine, kaempferol, and germacrene-A with these proteins provides ground for further research to understand the underlying mechanism that ceases the growth of these microbes.
Short-term responses of the anammox process to Ni(II): nitrogen removal, mechanisms and inhibition recovery
Authors: Li Yu-Qi , Zhao Bai-Hang , Sun Qi , Zhang Jing , Zhang Yu-Qing , Li Jun 
Published: July 2022

AbstractAnaerobic ammonia oxidizing (anammox) has already been recognized as an innovative and economical nitrogen removal technology. However, the effect of heavy metals on anammox bacteria in aquatic ecosystem remains largely unknown. Ni(II) is a common kind of heavy metals detected in industrial wastewater and municipal sewage treatment plants. Hence, the responses of the anammox process to Ni(II) were studied here. The results showed that anammox was the dominant reaction with Ni(II) concentrations no more than 25 mg/L. 1 mg/L of Ni(II) addition promoted nitrogen removal by anammox. The higher the Ni(II) concentrations and longer exposure time, the more inhibition for anammox bacteria was gotten. The IC50 of Ni(II) to anammox was determined as 83.86 mg/L by an exponential regression equation. The inhibition of Ni(II) on anammox activity was mainly attributed to intracellular accumulation Ni(II) inhibition to HDH activity. Two times increase of IC50 after 4 times circles of domestication suggests multiple intermittent domestication can increase the tolerance of anammox bacteria to Ni(II). EDTA washing can eliminate the inhibition of anammox activity by Ni(II) with Ni(II) addition no more than 25 mg/L.
Multisensory interactions on auditory and somatosensory information in expert pianists
Authors: Hirano Masato , Furuya Shinichi 
Published: July 2022

AbstractFine-tuned sensory functions typically characterize skilled individuals. Although numerous studies demonstrated enhanced unimodal sensory functions at both neural and behavioral levels in skilled individuals, little is known about their multisensory interaction function, especially multisensory integration and selective attention that involve volitional control of information derived from multiple sensory organs. In the current study, expert pianists and musically untrained individuals performed five sets of intensity discrimination tasks at the auditory and somatosensory modalities with different conditions: (1) auditory stimulus, (2) somatosensory stimulus, (3) congruent auditory and somatosensory stimuli (i.e., multisensory integration), (4) auditory and task-irrelevant somatosensory stimuli, and (5) somatosensory and task-irrelevant auditory stimuli. In the fourth and fifth conditions, participants were instructed to ignore a task-irrelevant stimulus and to pay attention to a task-relevant stimulus (i.e., selective attention), respectively. While the discrimination perception was superior in the condition (3) compared to the better one of the individual unimodal conditions only in the pianists, the task-irrelevant somatosensory stimulus worsened the auditory discrimination more in the pianists than the nonmusicians. These findings indicate unique multisensory interactions in expert pianists, which enables pianists to efficiently integrate the auditory and somatosensory information, but exacerbates top-down selective inhibition of somatosensory information during auditory processing.
New systematic study approach of green synthesis CdS thin film via Salvia dye
Authors: Najm A. S. , Naeem Hasanain Salah , Alabboodi Khalid O. , Hasbullah Siti Aishah , Hasan Hiba Ali , Holi Araa Mebdir , AL-Zahrani Asla Abdullah , Sopian K. , Bais Badariah , Majdi Hasan Sh. , Sultan Abbas J. 
Published: July 2022

AbstractIn this study, we aimed to increase the knowledge regarding the response mechanisms which were associated with the formation of CdS thin films. CdS thin film remains the most appealing alternative for many researchers, as it has been a capable buffer material for effect in film based polycrystalline solar cells (CdTe, CIGSe, CZTS). The Linker Assisted and Chemical Bath Deposition (LA-CBD) technique, which combines the Linker Assisted (LA) technique and the chemical bath deposition (CBD) method for forming high quality CdS thin film, was presented as an efficient and novel hybrid sensitization technique. CdS films were bound to soda lime with the help of electrostatic forces, which led to the formation of the intermediate complexes [Cd (NH3)4]2+that helped in the collision of these complexes with a soda lime slide. Salvia dye and as a linker molecule 3-Mercaptopropionic acid (MPA) was used in the one step fabrication technique. Optical results showed that the bandgap varied in the range of (2.50 to 2.17) eV. Morphological properties showed a homogeneous distribution of the particles that aspherical in shape in the CdS + MPA + Salvia dye films. This technique significantly affected on the electrical characterizations of CdS films after the annealing process. The CdS + Ag + MPA + Salvia dye films showed the maximum carrier concentration and minimum resistivity, as 5.64 × 1018 cm−3and 0.83 Ω cm respectively.
Logic and memory functions of an inverter comprising reconfigurable double gated feedback field effect transistors
Authors: Jeon Juhee , Woo Sola , Cho Kyoungah , Kim Sangsig 
Published: July 2022

AbstractIn this study, we propose an inverter consisting of reconfigurable double-gated (DG) feedback field-effect transistors (FBFETs) and examine its logic and memory operations through a mixed-mode technology computer-aided design simulation. The DG FBFETs can be reconfigured to n- or p-channel modes, and these modes exhibit an on/off current ratio of ~ 1012 and a subthreshold swing (SS) of ~ 0.4 mV/dec. Our study suggests the solution to the output voltage loss, a common problem in FBFET-based inverters; the proposed inverter exhibits the same output logic voltage as the supply voltage in gigahertz frequencies by applying a reset operation between the logic operations. The inverter retains the output logic ‘1’ and ‘0’ states for ~ 21 s without the supply voltage. The proposed inverter demonstrates the promising potential for logic-in-memory application.
Impact on the reduction of CO2 emissions due to the use of telemedicine
Authors: Morcillo Serra César , Aroca Tanarro Ana , Cummings Catherine Mary , Jimenez Fuertes Araceli , Tomás Martínez José Francisco 
Published: July 2022

AbstractDigital health can reduce CO2 emissions thanks to telemedicine and access to digital test results and medical reports. However, the environmental impact of digital health activity is not well known. Here, we show that telemedicine reduces CO2 emissions. We found a net total of 6,655 tons of CO2 emissions decrease through a reduction in patient travel to surgeries and medical clinics thanks to the alternatives of digital appointments and digital access to test results and medical reports, which avoid the need to travel to a clinic for a face-to-face visit or to pick up printed results or reports. During 2020, a total of 640,122 digital appointments were carried out by the health care company, which avoided 1,957 net tons of CO2 emissions, while patients downloaded 3,064,646 digital medical reports through the company portal, which avoided an additional 4,698 net tons of CO2 emissions. Our results demonstrate how digital appointments and digital reports, reduce CO2 emissions by reducing the need for patient travel.
Leveraging plant physiological dynamics using physical reservoir computing
Authors: Pieters Olivier , De Swaef Tom , Stock Michiel , wyffels Francis 
Published: July 2022

AbstractPlants are complex organisms subject to variable environmental conditions, which influence their physiology and phenotype dynamically. We propose to interpret plants as reservoirs in physical reservoir computing. The physical reservoir computing paradigm originates from computer science; instead of relying on Boolean circuits to perform computations, any substrate that exhibits complex non-linear and temporal dynamics can serve as a computing element. Here, we present the first application of physical reservoir computing with plants. In addition to investigating classical benchmark tasks, we show thatFragaria × ananassa(strawberry) plants can solve environmental and eco-physiological tasks using only eight leaf thickness sensors. Although the results indicate that plants are not suitable for general-purpose computation but are well-suited for eco-physiological tasks such as photosynthetic rate and transpiration rate. Having the means to investigate the information processing by plants improves quantification and understanding of integrative plant responses to dynamic changes in their environment. This first demonstration of physical reservoir computing with plants is key for transitioning towards a holistic view of phenotyping and early stress detection in precision agriculture applications since physical reservoir computing enables us to analyse plant responses in a general way: environmental changes are processed by plants to optimise their phenotype.
Evaluation of the contact surface between vertebral endplate and 3D printed patient-specific cage vs commercial cage
Authors: Fernandes Renan Jose Rodrigues , Gee Aaron , Kanawati Andrew James , Siddiqi Fawaz , Rasoulinejad Parham , Zdero Radovan , Bailey Christopher Stewart 
Published: July 2022

AbstractBiomechanical study. To evaluate the performance of the contact surface for 3D printed patient-specific cages using CT-scan 3D endplate reconstructions in comparison to the contact surface of commercial cages. Previous strategies to improve the surface of contact between the device and the endplate have been employed to attenuate the risk of cage subsidence. Patient-specific cages have been used to help, but only finite-element studies have evaluated the effectiveness of this approach. There is a possible mismatch between the CT-scan endplate image used to generate the cage and the real bony endplate anatomy that could limit the performance of the cages. A cadaveric model is used to investigate the possible mismatch between 3D printed patient-specific cages and the endplate and compare them to commercially available cages (Medtronic Fuse and Capstone). Contact area and contact stress were used as outcomes. When PS cage was compared to the Capstone cage, the mean contact area obtained was 100 ± 23.6 mm2 and 57.5 ± 13.7 mm2, respectively (p < 0.001). When compared to the Fuse cage, the mean contact area was 104.8 ± 39.6 mm2 and 55.2 ± 35.1 mm2, respectively(p < 0.001). Patient-specific cages improve the contact area between the implant and the endplate surface, reducing the contact stress and the risk of implant subsidence during LIF surgeries.
Real intensity of physical activity capacity of patients with chronic disease: a cross-sectional study
Authors: Allado Edem , Poussel Mathias , Albuisson Eliane , Paysant Jean , Temperelli Margaux , Hily Oriane , Moussu Anthony , Benhajji Noura , Gauchard Gerôme , Chenuel Bruno 
Published: July 2022

AbstractThe aim of this study was to evaluate the real intensity level of exercise in a sample of patients with chronic disease from obesity, rheumatology, hematology and other departments involved in a hospital-based program of adapted physical activity (APA). For this cross-sectional study, we studied seventy-five patients with chronic disease and no beta-blocker treatment. They systematically performed a cardiopulmonary exercise test before participating in a supervised APA practice using a telemetry wireless system to monitor heart rate (HR) during the first session. Based upon the results of the functional evaluation of exercise performance, we studied two groups of patients: (1) No limitation in exercise performance (maximal oxygen uptake greater than or equal to 80% of the theoretical reference) and (2) limited exercise performance (maximal oxygen uptake less than 80% of the theoretical value). Fifty-two patients (69.3%) were women, mean age was 42.6 (± 13.8), and mean BMI was 36.7 (± 10.6). Most patients had been referred for obesity (57.3%). We found 39 patients with normal exercise capacities and 36 patients with limited exercise performance. There were no significant differences in demographic and clinical characteristics between the two groups. For all populations, the mean and median real intensity levels of exercise in a sample of patients were moderate (55–70% HR max) and were the same for both groups. During the most intensive 15-min bout of the APA session, the HR for patients in both groups was greater than 70% of the actual maximum HR. This study observed a moderate level of APA exercise intensity in patients suffering from various chronic diseases. We found no significant difference in intensity level of exercise between patients’ capacities, i.e., with and without limitation of their maximal performance.