bluuebunny/crossref_metadata_2025_split huggingface dataset:
Body fat composition determines outcomes before and after liver transplantation in patients with cirrhosis
Authors: Engelmann Cornelius , Aehling Niklas F. , Schob Stefan , Nonnenmacher Ines , Handmann Luise , Macnaughtan Jane , Herber Adam , Surov Alexey , Kaiser Thorsten , Denecke Timm , Jalan Rajiv , Seehofer Daniel , Moche Michael , Berg Thomas 
Published: April 2022

Abstract
Cachexia occurs in late stages of liver cirrhosis, and a low‐fat mass is potentially associated with poor outcome. This study compared different computed tomography (CT)–derived fat parameters with respect to its prognostic impact on the development of complications and death before and after liver transplantation. Between 2001 and 2014, 612 patients with liver cirrhosis without hepatocellular carcinoma listed for liver transplantation met the inclusion criteria, including abdominal CT scan (±200 days to listing). A total of 109 patients without cirrhosis served as controls. The subcutaneous fat index (SCFI), the paraspinal muscle fat index, and the visceral fat index were assessed at L3/L4 level and normalized to the height (cm2/m2). Data were collected and analyzed retrospectively. Low SCFI was associated with a higher rate of ascites and increased C‐reactive protein levels (p < 0.001). In addition, multivariate Cox regression analysis adjusting for sex, age, body mass index (BMI), and Model for End‐Stage Liver Disease showed that decreasing SCFI was also associated with an increased risk of cirrhosis‐related complications (p = 0.003) and death on the transplant wait list (p = 0.013). Increased paraspinal and visceral fat were not only positively correlated with creatinine levels (p < 0.001), BMI, and metabolic comorbidities (all p < 0.001) before transplantation, but also predictive for 1‐year mortality after transplantation. Conclusion: The distribution of body fat is a major determinant for complications and outcome in cirrhosis before and after liver transplantation.
Applying after action review to examine residential treatment programs' responses to COVID‐19
Authors: Kim Bo , Petrakis Beth Ann , McInnes D. Keith , Gifford Allen L. , Sliwinski Samantha K. , Smelson David A. 
Published: April 2022

AbstractResurgences of COVID‐19 cases are a grave public health concern. Hence, there is an urgent need for health care systems to rapidly and systematically learn from their responses to earlier waves of COVID‐19. To meet this need, this article delineates how we adapted the World Health Organization's After Action Review (AAR) framework to use within our health care system of the United States Department of Veterans Affairs. An AAR is a structured, methodical evaluation of actions taken in response to an event (e.g., recent waves of COVID‐19). It delivers an actionable report regarding (i) what was expected, (ii) what actually happened, (iii) what went well, and (iv) what could have been done differently, and thus what changes are needed for future situations. We share as an example our examination of Mental Health Residential Rehabilitation and Treatment Programs in Massachusetts (a COVID‐19 hotspot). Our work can be further adapted, beyond residential treatment, as a consistent framework for reviewing COVID‐19 responses across multiple health care programs. This will identify both standardized and tailored preparations that the programs can make for future waves of the pandemic. Given the expected resurgences of COVID‐19 cases, the time to apply AAR is now.
Yucatec Maya mothers' ethnotheories about learning to help at home
Authors: Alcalá Lucía , Cervera María Dolores 
Published: April 2022

AbstractIn most cultures, but particularly among Indigenous communities of the Americas, children help extensively with household work. However, less is known about the role of maternal ethnotheories as cultural organizers of the family environment and children's helping. We explored Maya maternal ethnotheories about children's learning to help in two villages. Mothers reported that children learn by observing and pitching in because they have the will and interest and want to help. Mothers help children by orchestrating the setting, supporting their initiative, but learning must be initiated by children. Results support the view of a Yucatec Maya theory of learning and highlight the relevance of studying maternal ethnotheories. As cultural models, ethnotheories guide the organization of the developmental niche, affording children's learning by observing and pitching in and supporting the emergence of their will and interest. Their study may contribute to further acknowledging and supporting cultural diversity.
Deep machine learning unravels the structural origin of mid‐gap states in chalcogenide glass for high‐density memory integration
Authors: Xu Meng , Xu Ming , Miao Xiangshui 
Published: April 2022

AbstractThe recent development of three‐dimensional semiconductor integration technology demands a key component—the ovonic threshold switching (OTS) selector to suppress the current leakage in the high‐density memory chips. Yet, the unsatisfactory performance of existing OTS materials becomes the bottleneck of the industrial advancement. The sluggish development of OTS materials, which are usually made from chalcogenide glass, should be largely attributed to the insufficient understanding of the electronic structure in these materials, despite of intensive research in the past decade. Due to the heavy first‐principles computation on disordered systems, a universal theory to explain the origin of mid‐gap states (MGS), which are the key feature leading to the OTS behavior, is still lacking. To avoid the formidable computational tasks, we adopt machine learning method to understand and predict MGS in typical OTS materials. We build hundreds of chalcogenide glass models and collect major structural features from both short‐range order (SRO) and medium‐range order (MRO) of the amorphous cells. After training the artificial neural network using these features, the accuracy has reached ~95% when it recognizes MGS in new glass. By analyzing the synaptic weights of the input structural features, we discover that the bonding and coordination environments from SRO and particularly MRO are closely related to MGS. The trained model could be used in many other OTS chalcogenides after minor modification. The intelligent machine learning allows us to understand the OTS mechanism from vast amount of structural data without heavy computational tasks, providing a new strategy to design functional amorphous materials from first principles.image
Polyethylene terephthalate textile heart valve: How poly(ethylene glycol) grafting limits fibrosis
Authors: Amri Amna , Chevallier Pascale , Guay‐Bégin Andrée‐Anne , Bilem Ibrahim , Gauvin Guillaume , Alamdari Houshang , Heim Frédéric , Laroche Gaétan 
Published: April 2022

AbstractTranscatheter aortic valve replacement (TAVR) is an alternative technique to surgical valve replacement for over 300,000 patients worldwide. The valve material used in the TAVR is made of biological tissues, whose durability remains unknown. The success of the TAVR favors the research toward synthetic valve leaflet materials as an alternative to biological tissues. In particular, polyethylene terephthalate (PET) textile valves have recently proven durability over a 6‐month period in animal sheep models. Excessive fibrotic tissue formation remains, however, a critical issue to be addressed. The aim of this work was therefore to investigate the potential of PET textiles covalently conjugated with polyethylene glycol (PEG), known for its antifouling properties, to modulate the fibrosis formation both in vitro and in vivo. For this purpose, the surfaces of heart valves made of PET textiles were functionalized with an atmospheric pressure plasma, leading to the formation of carboxylic acid (COOH) groups, further used for PEG‐NH2 conjugation. Surface modification efficiency was assessed by X‐ray photoelectron spectroscopy and water contact angle measurements. The biological behavior of the as‐modified surfaces was evaluated by in vitro assays, using rat cardiac fibroblast cells. The results show that PEG treated substrates restrained the fibroblasts adhesion and proliferation. The PEG treated valve, implanted in a juvenile sheep model, showed a significant fibrosis reduction. The explant also revealed calcification issues that need to be addressed.
Reduced Bone Mass in Collagen Prolyl 4‐Hydroxylase P4ha1+/−; P4ha2−/− Compound Mutant Mice
Authors: Tolonen Jussi‐Pekka , Salo Antti M , Finnilä Mikko , Aro Ellinoora , Karjalainen Emma , Ronkainen Veli‐Pekka , Drushinin Kati , Merceron Christophe , Izzi Valerio , Schipani Ernestina , Myllyharju Johanna 
Published: April 2022

ABSTRACTProper deposition of the extracellular matrix and its major components, the collagens, is essential for endochondral ossification and bone mass accrual. Collagen prolyl 4‐hydroxylases (C‐P4Hs) hydroxylate proline residues in the ‐X‐Pro‐Gly‐ repeats of all known collagen types. Their product, 4‐hydroxyproline, is essential for correct folding and thermal stability of the triple‐helical collagen molecules in physiological body temperatures. We have previously shown that inactivation of the mouse P4ha1 gene, which codes for the catalytic α subunit of the major C‐P4H isoform, is embryonic lethal, whereas inactivation of the P4ha2 gene produced only a minor phenotype. Instead, mice with a haploinsufficiency of the P4ha1 gene combined with a homozygous deletion of the P4ha2 gene present with a moderate chondrodysplasia due to transient cell death of the growth plate chondrocytes. Here, to further characterize the bone phenotype of the P4ha1+/−; P4ha2−/− mice, we have carried out gene expression analyses at whole‐tissue and single‐cell levels, biochemical analyses, microcomputed tomography, histomorphometric analyses, and second harmonic generation microscopy to show that C‐P4H α subunit expression peaks early and that the C‐P4H deficiency leads to reduced collagen amount, a reduced rate of bone formation, and a loss of trabecular and cortical bone volume in the long bones. The total osteoblast number in the proximal P4ha1+/−; P4ha2−/− tibia and the C‐P4H activity in primary P4ha1+/−; P4ha2−/− osteoblasts were reduced, whereas the population of osteoprogenitor colony‐forming unit fibroblasts was increased in the P4ha1+/−; P4ha2−/− marrow. Thus, the P4ha1+/−; P4ha2−/− mouse model recapitulates key aspects of a recently recognized congenital connective tissue disorder with short stature and bone dysplasia caused by biallelic variants of the human P4HA1 gene. Altogether, the data demonstrate the allele dose‐dependent importance of the C‐P4Hs to the developing organism and a threshold effect of C‐P4H activity in the proper production of bone matrix. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
On the highest oxidation states of the actinoids in AnO4 molecules (An = Ac – Cm): A DMRG‐CASSCF study
Authors: Lu Jun‐Bo , Jiang Xue‐Lian , Wang Jia‐Qi , Hu Han‐Shi , Schwarz W. H. Eugen , Li Jun 
Published: April 2022

AbstractActinoid tetroxide molecules AnO4 (An = Ac – Cm) are investigated with the ab initio density matrix renormalization group (DMRG) approach. Natural orbital shapes are used to read out the oxidation state (OS) of the f‐elements, and the atomic orbital energies and radii are used to explain the trends. The highest OSs reveal a “volcano”‐type variation: For An = Ac – Np, the OSs are equal to the number of available valence electrons, that is, AcIII, ThIV, PaV, UVI, and NpVII. Starting with plutonium as the turning point, the highest OSs in the most stable AnO4 isomers then decrease as PuV, AmV, and CmIII, indicating that the 5f‐electrons are hard to be fully oxidized off from Pu onward. The variations are related to the actinoid contraction and to the 5f‐covalency characteristics. Combined with previous work on OSs, we review their general trends throughout the periodic table, providing fundamental understanding of OS‐relevant phenomena.
LncRNA TUG1 functions as a ceRNA for miR‐1‐3p to promote cell proliferation in hepatic carcinogenesis
Authors: Tang Keke , Lv Di , Miao Lingling , Mao Yushan , Yu Xiaoyan 
Published: April 2022

AbstractBackgroundHepatocellular carcinoma (HCC) is characterised by high malignancy, metastasis and recurrence, but the specific mechanism that drives these outcomes is unclear. Recent studies have shown that long noncoding RNAs (lncRNAs) can regulate the proliferation and apoptosis of hepatic cells.MethodsWe searched for lncRNAs and microRNAs (miRNAs), which can regulate IGF1 expression, through a bioinformatics website, and predicted that lncRNA taurine‐upregulated gene 1 (TUG1) would have multiple targets for miR‐1‐3p binding, meaning that lncRNA TUG1 played an adsorption role. A double luciferase assay was used to verify the targeting relationship between lncRNA TUG1 and miR‐1‐3p. Western blotting and qPCR were used to verify the targeting relationship between miR‐1‐3p and IGF1, and qPCR was used to verify the regulatory relationship between the lncRNA TUG1‐miR‐1‐3p‐IGF1 axis. CCK‐8 was used to detect the growth activity of miRNA‐transfected L‐O2 cells, and flow cytometry was used to detect cell cycle changes and apoptosis.ResultThe proliferation cycle of L‐O2 cells transfected with miR‐1‐3p mimics was significantly slowed. Flow cytometry showed that the proliferation of L‐O2 cells was slowed, and the apoptosis rate was increased. In contrast, when L‐O2 cells were transfected with miR‐1‐3p inhibitor, the expression of IGF1 was significantly upregulated, and the cell proliferation cycle was significantly accelerated. Flow cytometry showed that the cell proliferation rate was accelerated, and the apoptosis rate was reduced.ConclusionLncRNA TUG1 can adsorb miR‐1‐3p as a competitive endogenous RNA (ceRNA) to promote the expression of IGF1 and promote cell proliferation in hepatic carcinogenesis.
An overview on inactivated and live‐attenuated SARS‐CoV‐2 vaccines
Authors: Khoshnood Saeed , Arshadi Maniya , Akrami Sousan , Koupaei Maryam , Ghahramanpour Hossein , Shariati Aref , Sadeghifard Nourkhoda , Heidary Mohsen 
Published: April 2022

AbstractAfter about 2 years since severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2), first infections were detected in Wuhan city of China in December 2019, which was followed by a worldwide pandemic with a record of 5.41 million deaths. Due to urgent need for the development of a safe and effective vaccine for coronavirus disease 2019 (COVID‐19), attempts for producing efficient vaccines are inexhaustibly continuing. According to a report by the World Health Organization (WHO) on COVID‐19 vaccine tracker and landscape, there are 149 vaccine candidates all over the world. Inactivated SARS‐CoV‐2 vaccines as a conventional vaccine platform consist of whole virus particles grown in cell culture and inactivated by chemicals. Because of benefits such as antigenic similarity to real virion inducing humoral and cellular immune responses and ease for transport and storage, these vaccines, including the vaccines produced by Bharat Biotech, Sinopharm, and Sinovac, are in use at large scales. In this study, we have a review on inactivated SARS‐CoV‐2 vaccines that are passing their phase 3 and 4 clinical trials, population which was included in the trials, vaccine producers, the efficiency, adverse effects, and components of vaccines, and other vaccine features.
Prevalence of cardiovascular risk factors by HIV status in a population‐based cohort in South Central Uganda: a cross‐sectional survey
Authors: Enriquez Rocio , Ssekubugu Robert , Ndyanabo Anthony , Marrone Gaetano , Gigante Bruna , Chang Larry W. , Reynolds Steven J. , Nalugoda Fred , Ekstrom Anna Mia , Sewankambo Nelson K. , Serwadda David M. , Nordenstedt Helena 
Published: April 2022

AbstractIntroductionCardiovascular disease is one of the leading causes of mortality for people living with HIV, but limited population‐based data are available from sub‐Saharan Africa. This study aimed to determine the prevalence of key cardiovascular disease risk factors, 10‐year risk of cardiovascular disease and type 2 diabetes mellitus through risk scores by HIV status, as well as investigate factors associated with hyperglycaemia, hypertension and dyslipidaemia in South‐Central Uganda.MethodsA cross‐sectional study was conducted in 37 communities of the population‐based Rakai Community Cohort Study from May 2016 to May 2018. In total, 990 people living with HIV and 978 HIV‐negative participants aged 35–49 years were included. Prevalence estimates and 10‐year cardiovascular and type 2 diabetes risk were calculated by sex and HIV serostatus. Multivariable logistic regression was used to determine associations between socio‐demographic, lifestyle and body composition risk factors and hyperglycaemia, hypertension and dyslipidaemia.ResultsOverweight (21%), obesity (9%), abdominal obesity (15%), hypertension (17%) and low high‐density lipoprotein (HDL) (63%) were the most common cardiovascular risk factors found in our population. These risk factors were found to be less common in people living with HIV apart from hypertension. Ten‐year risk for cardiovascular and type 2 diabetes mellitus risk was low in this population with <1% categorized as high risk. In HIV‐adjusted multivariable analysis, obesity was associated with a higher odds of hypertension (odds ratio [OR] = 2.31, 95% confidence interval [CI] 1.35–3.96) and high triglycerides (OR = 2.08, CI 1.25–3.47), and abdominal obesity was associated with a higher odds of high triglycerides (OR = 2.55, CI 1.55–4.18) and low HDL (OR = 1.36, CI 1.09–1.71). A positive HIV status was associated with a lower odds of low HDL (OR = 0.43, CI 0.35–0.52).ConclusionsIn this population‐based study in Uganda, cardiovascular risk factors of obesity, abdominal obesity, hypertension and dyslipidaemia were found to be common, while hyperglycaemia was less common. Ten‐year risk for cardiovascular and type 2 diabetes mellitus risk was low. The majority of cardiovascular risk factors were not affected by HIV status. The high prevalence of dyslipidaemia in our study requires further research.
Risks of catching COVID‐19 according to vaccination status of healthcare workers during the SARS‐CoV‐2 Delta variant dominant period and their clinical characteristics
Authors: Özüdoğru Osman , Acer Ömer , Genç Bahçe Yasemin 
Published: April 2022

AbstractThe exposure of healthcare workers (HCWs) to severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) has been a major concern since the beginning of the coronavirus disease 2019 (COVID‐19) pandemic. The study aimed to investigate the relationship between vaccination status and the status of catching COVID‐19 in HCWs working in a Training and Research Hospital in Turkey, and the clinical course of the disease in those who were caught. The vaccination status of 1279 HCWs working at Siirt Training and Research Hospital during the period when the SARS‐CoV‐2 Delta variant was dominant, their cases of catching COVID‐19 during this period, and the clinical course of the disease in patients with COVID‐19 were investigated retrospectively. We found that the rate of COVID‐19 transmission was lowest in fully vaccinated HCWs (p < 0.05). The rate of COVID‐19 transmission in HCWs who received two doses of BioNTech vaccine (4.4%) and two doses of CoronaVac+ one dose of BioNTech vaccines (2.7%) was considerably lower than those without vaccination (26.2%) (p < 0.05). The transmission rate was lowest among those vaccinated with two doses of CoronaVac+ one dose of BioNTech. Hospitalization was not required in fully vaccinated HCWs. The lymphocyte count was found to be significantly higher in fully vaccinated patients than incompletely vaccinated and unvaccinated patients. Although C‐reactive protein (CRP), d‐dimer, and ferritin values were higher in unvaccinated and partially vaccinated patients than in fully vaccinated patients, the differences were not statistically significant. As a result, the transmission rate of COVID‐19 was lowest in fully vaccinated HCWs and in those vaccinated with two doses of CoronaVac+ one dose of BioNTech. In fully vaccinated HCWs, hospitalization was not needed.
Comparative detection, density, and reproductive performance of Kirtland's warbler in jack and red pine
Authors: Van Dyke Fred , Harju Seth , Hindy Michael , Cannata Natalie , Schmidt Emily , Hillman Erin , Sargent Alyssa , Keas Brian 
Published: April 2022

AbstractThe formerly endangered Kirtland's warbler (Setophaga kirtlandii) is among a growing number of conservation‐reliant species that depend on active management to avoid reverting to endangered status. Because the Kirtland's warbler is a habitat specialist of young, even‐aged jack pine (Pinus banksiana), managers of the recovery effort stressed creating new jack pine stands and monitoring numbers of singing males through an annual census using single visits to individual stands. Kirtland's warbler will occupy and breed in red pine (P. resinosa), but red pine has not been surveyed for Kirtland's warblers in the annual population census. Furthermore, the current monitoring approach cannot determine their species detection probability or individual detection probability, which is essential to evaluate both red pine use and the accuracy of the census. From 2016–2018 we estimated density and detection probabilities in jack pine and red pine stands through repeated visits to a limited number of stands rather than single visits to many stands. Estimates of species detection probability indicated that ≥1 male Kirtland's warbler would be detected on most sites when any were present, but individual detection probabilities were less and varied by stand type, indicating that single visits to sites would underestimate numbers and that accurate estimation of detection probability was important for estimation of density in different stand types. We offer quantitative estimates of detection probabilities for determination of Kirtland's warbler population size in jack pine versus red pine stands in the same areas and breeding seasons. Managers of Kirtland's warblers should incorporate detection probabilities into population surveys to achieve more accurate estimates of population size.