bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation of serum anti-Mullerian hormone with hormonal and environmental parameters in Brazilian climacteric women
Authors: Gouvea Thiago Magalhães , Cota e Souza Laura Alves , Lima Angélica Alves 
Published: July 2022

AbstractThis study aimed to identify the correlation among anti-Mullerian Hormone serum levels and 25-OH-D, obesity, metabolic syndrome (MetS), and sexual hormones in climacteric women classified according to stages of reproductive aging (SRA). A cross-sectional study was conducted with a total of 177 Brazilian climacteric women between 40 and 64 years old. Concentrations of AMH were measured using the Access 2 Immunoassay System. A multiple linear regression analysis was used to identify the relationship among AMH, 25-OH-D, obesity, MetS, sexual hormones, sociodemographic and lifestyle factors. AMH levels decreased with increased age (B = − 0.059; p < 0.001), and reproductive aging (B = − 0.483; p < 0.001). Obesity indicators, lifestyle characters, 25-OH-D levels and MetS were not significantly associated with AMH serum concentration. Negative correlation was found for FSH (B = − 0.009; p < 0.001) and LH (B = − 0.006; p = 0.004); positive correlation for E2 (B = 0.001; p = 0.011), DHEAS (B = 0.003; p < 0.001) and SHBG (B = 0.003; p = 0.005). In the model adjusted for SRA, FSH levels (p < 0.001) and DHEAS (p = 0.014) were associated with AMH. Although, with the adjustment for age, only FSH remained with a significant association (p = 0.001). Of the other analytes, none was associated with AMH, regardless of the model fit. Our findings confirm that serum AMH level decreased with age and FSH levels, but there is no correlation between AMH with obesity, 25-OH-D, MetS or other sexual hormones in Brazilian climacteric women.
Circ_0000181 regulates miR-667-5p/NLRC4 axis to promote pyroptosis progression in diabetic nephropathy
Authors: Li Yining , Yu Weihong , Xiong Hao , Yuan Fang 
Published: July 2022

AbstractOur previous research demonstrated that NOD-like receptor family CARD domain-containing protein 4 (NLRC4) inflammasome was overexpressed in renal tissues of patients with diabetic nephropathy (DN). This study further investigated the effect of circRNAs-miRNAs interaction on NLRC4 and their potential mechanisms. DN mice models were first established using STZ. Then, pyroptosis related marker expression was detected using qPCR, western blot (WB), and immunohistochemistry analysis. After that, differentially expressed circRNAs, miRNAs, and mRNAs were investigated using next-generation sequencing. Additionally, the function and potential mechanism of circ_0000181 and miR-667-5p on pyroptosis were measured in vitro DN cell model using MTS, WB, and Enzyme-linked immunosorbent assay. There was an apparent elevation of NLRC4, Caspase1, IL-1β, and IL-18 levels in DN mice. The next-generation sequencing results revealed that there were 947 circRNAs and 390 miRNAs significantly different between the DN and sham kidney tissue, of which circ_0000181 and miR-667-5p had potential targeting effects with NLRC4. Dual-luciferase and functional rescue experiments demonstrated that circ_0000181 promoted NLRC4 inflammasome activation via competitive sponge of miR-667-5p, promoted the release of IL-1β and IL-18, and caused pyroptosis. Altogether, circ_0000181 regulates miR-667-5p/NLRC4 axis to promote pyroptosis progression in DN.
Nitrogen balance and efficiency as indicators for monitoring the proper use of fertilizers in agricultural and livestock systems
Authors: Oliveira Joyce Graziella , Luiz Santana Júnior Mário , Jaqueline Costa Maia Nayane , Batista Dubeux Junior José Carlos , Hauber Gameiro Augusto , Kunrath Taise Robinson , Geraldi Mendonça Gabriela , Fernanda Simili Flávia 
Published: July 2022

AbstractThe rational use of nutrients is a key factor for the sustainability of agricultural systems. This study aimed to analyze the nitrogen balance and use efficiency, and the valorization of organic residues within integrated systems, in comparison to conventional agricultural and livestock systems. The experiment was assembled in a randomized blocks design with three replicates. Six production systems were compared, grain maize production (CROP) and pasture for beef cattle production (LS), and four ICLS (Integrated Crop-Livestock System) for grain maize and pastures for beef cattle, in 2 years. In order to estimate the nutrients balance, inputs, and outputs at farm levels were considered, and with the results obtained for nutrient balance, the use efficiency was calculated. The CROP presented higher nutrient use efficiency (1.43 kg/ha−1), but at the same time, it resulted in negative contributions for the nutrient balance (−97 kg/ha−1) because of lower amounts of nitrogen in the organic residues (188 kg/ha−1) and lower valuation. The LS and ICLS provided a higher amount of nitrogen (983 kg/ha−1; mean ± 921 kg/ha−1) and valuation of organic residues. The presence of components such as pastures and the animal contribute to a positive production system, while reducing the needs for chemical fertilizers.
Prefrontal projections to the nucleus reuniens signal behavioral relevance of stimuli during associative learning
Authors: Yu Xiaotian , Jembere Fasika , Takehara-Nishiuchi Kaori 
Published: July 2022

AbstractThe nucleus reuniens (RE) is necessary for memories dependent on the interaction between the medial prefrontal cortex (mPFC) and hippocampus (HPC). One example is trace eyeblink conditioning, in which the mPFC exhibits differential activity to neutral conditioned stimuli (CS) depending on their contingency with an aversive unconditioned stimulus (US). To test if this relevancy signal is routed to the RE, we photometrically recorded mPFC axon terminals within the RE and tracked their changes with learning. As a comparison, we measured prefrontal terminal activity in the mediodorsal thalamus (MD), which lacks connectivity with the HPC. In naïve male rats, prefrontal terminals within the RE were not strongly activated by tone or light. As the rats associated one of the stimuli (CS+) with the US, terminals gradually increased their response to the CS+ but not the other stimulus (CS-). In contrast, stimulus-evoked responses of prefrontal terminals within the MD were strong even before conditioning. They also became augmented only to the CS+ in the first conditioning session; however, the degree of activity differentiation did not improve with learning. These findings suggest that associative learning selectively increased mPFC output to the RE, signaling the behavioral relevance of sensory stimuli.
Unsteady MHD free convection flow of an exothermic fluid in a convectively heated vertical channel filled with porous medium
Authors: Hamza Muhammed Murtala , Shuaibu Abdulsalam , Kamba Ahmad Samaila 
Published: July 2022

AbstractUtilizing porous media in a new mathematical model to improve convective heat transfer characteristics in a variety of applications, such as radiation nuclear disposal storing, evaporation cooling, sieving, geological extraction, crude petroleum refining, and building heating and cooling, is becoming increasingly important. This study proposed a numerical analysis of the unsteady magnetohydrodynamic free convection flow of an exothermic fluid with Newtonian heating. This discovery reveals two types of solutions: steady state and unsteady state. After transforming the governing equation from dimensional form to dimensionless form, the steady state governing equation was solved by the Homotopy Perturbation Method. However, the implicit finite difference approach is used to solve the time-dependent governing equations numerically. The impact of various emerging parameters, namely the Hartmann number, Boit number, Darcy number, Navier slip parameter, and the Frank-Kamenetskii parameter, was discussed and graphically analyzed. During the computations and analysis, it was discovered that a minor rise in the Hartman number results in the Lorentz force, which streamlines the momentum barrier layer and hence slows the fluid flow. The fluid velocity, on the other hand, rose as the porous medium, thermal Biot number, slip parameter, and temperature field increased as the viscous reactive fluid parameter and Newtonian heating increased. The skin friction and Nusselt number were also examined and reported. By comparing the finding to an existing work, a great agreement was revealed.
Ceftriaxone resistance among patients at GAMBY teaching general hospital
Authors: Gelaw Litegebew Yitayeh , Bitew Aschalew Afework , Gashey Eneyew Mebratu , Ademe Misrak Neway 
Published: July 2022

AbstractCeftriaxone is a broad spectrum of widely used antibiotics as it is highly effective against Gram-negative and Gram-positive isolates. Research on Ceftriaxone resistance helps to know its current status. Hence, we aimed to identify the prevalence of Ceftriaxone resistance on bacteria isolated from clinical specimens among patients at GAMBY Teaching General Hospital Bahir Dar, Ethiopia. Hospital-based retrospective cross-sectional study was conducted at GAMBY teaching General Hospital from November 01, 2015, to December 30, 2020, on 402 clinical specimens. Ceftriaxone susceptibility tests were carried out using the Kirby-Bauer disc diffusion method. Descriptive statistics and chi-square tests were applied for the analysis. Escherichia coli 114 (28.4%), S. aureus 90 (22.4%), S. saprophyticus 42 (10.4%), and Klebsiella spp 42 (10.4%) were the predominant identified bacteria. The overall resistance of ceftriaxone was 230 (57.2%). Sex and type of the clinical specimens had significantly associated with its resistance whereas age was not associated with ceftriaxone resistance. Ceftriaxone resistance has been steadily increasing. Ceftriaxone resistance was high. Sex and type of the clinical specimens had significantly associated with its resistance. Prevention mechanisms to ceftriaxone resistance should be strictly implemented. The right drugs should be selected based on susceptibility patterns.
Developing microsatellite duplex PCR reactions for sterlet (Acipenser ruthenus) and their application in parentage identification
Authors: Wang Jun , Sun Zhiwei , Jiang Linlang , Hu Yacheng 
Published: July 2022

AbstractThe sterlet (Acipenser ruthenus) is one of the 27 sturgeon species and is well-known for its wide distribution and small body size in comparison to other sturgeons. For assessing the population genetics and parentage identification of sterlet, ten microsatellites developed for Chinese sturgeon and cross-amplified in sterlet were tested by 40 individuals of sterlet. The ten microsatellites were developed using transcriptome sequencing of Chinese sturgeon. The expected heterozygosity (HE), observed heterozygosity (HO), Shannon-Weiner diversity indices (H′) and polymorphic information content (PIC) of the 10 microsatellites ranged from 0.466 to 0.751, from 0.438 to 0.938, from 0.66 to 1.51 and from 0.368 to 0.716, respectively. Combined exclusion probability based on the genotype of pair parent known (CE-PP), one parent known (CE-2P), and no parent known (CE-1P) of the 10 microsatellites were 99.99%, 99.96%, and 99.49%, respectively. These result showed that the 10 microsatellites should be helpful for assessing the population genetics and parentage identification of sterlet.
A rationally identified panel of microRNAs targets multiple oncogenic pathways to enhance chemotherapeutic effects in glioblastoma models
Authors: Sadeghipour Negar , Kumar Sukumar Uday , Massoud Tarik F. , Paulmurugan Ramasamy 
Published: July 2022

AbstractGlioblastoma (GBM) is the most common malignant brain tumor. Available treatments have limited success because most patients develop chemoresistance. Alternative strategies are required to improve anticancer effects of current chemotherapeutics while limiting resistance. Successful targeting of microRNAs (miRNAs) as regulators of gene expression can help reprogram GBM cells to better respond to chemotherapy. We aimed to identify a panel of miRNAs that target multiple oncogenic pathways to improve GBM therapy. We first identified differentially expressed miRNAs and tested if their target genes play central roles in GBM signaling pathways by analyzing data in the Gene Expression Omnibus and The Cancer Genome Atlas databases. We then studied the effects of different combinations of these miRNAs in GBM cells by delivering synthetic miRNAs using clinically compatible PLGA-PEG nanoparticles prior to treatment with temozolomide (TMZ) or doxorubicin (DOX). The successful miRNA panel was tested in mice bearing U87-MG cells co-treated with TMZ. We identified a panel of five miRNAs (miRNA-138, miRNA-139, miRNA-218, miRNA-490, and miRNA-21) and their oncogenic targets (CDK6, ZEB1, STAT3, TGIF2, and SMAD7) that cover four different signaling pathways (cell proliferation and apoptotic signaling, invasion and metastasis, cytokine signaling, and stemness) in GBM. We observed significant in vitro and in vivo enhancement of therapeutic efficiency of TMZ and DOX in GBM models. The proposed combination therapy using rationally selected miRNAs and chemotherapeutic drugs is effective owing to the ability of this specific miRNA panel to better target multiple genes associated with the hallmarks of cancer.
Multiplexed paper-based assay for personalized antimicrobial susceptibility profiling of Carbapenem-resistant Enterobacterales performed in a rechargeable coffee mug
Authors: Oeschger Taylor , Kret Lauren , Erickson David 
Published: July 2022

AbstractThe increasing prevalence of antibiotic resistance threatens to make currently treatable bacterial diseases deadly again. As drug resistance rises, antibiotic susceptibility testing needs to adapt to allow for widespread, individualized testing. Paper-based diagnostics offer low-cost, disposable alternatives to traditional time consuming and costly in-house methods. Here, we describe a paper-based microfluidic device, called the Bac-PAC, capable of categorizing the antibiotic susceptibly of individual strains of Carbapenem-resistant Enterobacterales. Each chip provides a colored readout with actionable susceptibility classification of three antibiotics, thus maximizing the chances of identifying a viable therapy. We verified the technology on thirty bacterial strains with two dyes using six clinically relevant antibiotics. We demonstrated that the dried tests are stable for one month and can be incubated in a rechargeable coffee mug that reduces the need for external infrastructure.
Design of ultra-thin underwater acoustic metasurface for broadband low-frequency diffuse reflection by deep neural networks
Authors: Li Ruichen , Jiang Yutong , Zhu Rongrong , Zou Yijun , Shen Lian , Zheng Bin 
Published: July 2022

AbstractUnderwater acoustic metasurfaces have broad application prospects for the stealth of underwater objects. However, problems such as a narrow operating frequency band, poor operating performance, and considerable thickness at low frequencies remain. In this study a reverse design method for ultra-thin underwater acoustic metasurfaces for low-frequency broadband is proposed using a tandem fully connected deep neural network. The tandem neural network consists of a pre-trained forward neural network and a reverse neural network, based on which a set of elements with flat phase variation and an almost equal phase shift interval in the range of 700–1150 Hz is designed. A diffuse underwater acoustic metasurface with 60 elements was designed, showing that the energy loss of the metasurface in the echo direction was greater than 10 dB. Our work opens a novel pathway for realising low-frequency wideband underwater acoustic devices, which will enable various applications in the future.
Wideband RCS reduction due to plasma generated by radioactive nuclei for cylindrical object
Authors: Ramezani M. , Razavi R. 
Published: July 2022

AbstractRadar cross section reduction has been one of the most important research topics in recent years. Plasma-based stealth is a method of reducing the radar cross section, which dampens the electromagnetic waves and reduces the amount of return waves. In this paper, a coating of the radioactive nucleus $$^{241}{\mathrm{Am}}$$

241

Am

on the surface of the cylinder with a radius of 10 cm is considered and the range of the emitted alpha particles and the electron density generated in the air are obtained using the Geant4 code under standard temperature and pressure conditions. By finite element method solution, the radar cross section of the conductive cylindrical object has been simulated and extracted in the presence and absence of plasma created by alpha-particles. The obtained results show a reduction of 5–8 dB $$\mathrm{m}^2$$

m

2

in the radar cross section in the frequency range of 2–12 GHz for specific activity source of 1 Ci/$$\mathrm{cm}^2$$

cm

2

.
Advanced microstructure, morphology and CO gas sensor properties of Cu/Ni bilayers at nanoscale
Authors: Ghaderi Atefeh , Shafiekhani Azizollah , Solaymani Shahram , Ţălu Ştefan , da Fonseca Filho Henrique Duarte , Ferreira Nilson S. , Matos Robert Saraiva , Zahrabi Hadi , Dejam Laya 
Published: July 2022

AbstractIn this study, we investigated the morphology of synthesized Cu/Ni nanoparticles in trace of carbon sources by the co-deposition process of RF sputtering and RF-PECVD methods and localized surface plasmon resonance of CO gas sensing of Cu/Ni nanoparticles. The surface morphology was studied by analyzing 3D micrographs of atomic force microscopy using image processing techniques and fractal/multifractal analyses. The MountainsMap® Premium software with the two-way ANOVA (Variance analysis) and least-significant differences tests were used for statistical analysis. The surface nano-patterns have a local and global particular distribution. Experimental and simulated Rutherford backscattering spectra confirm the quality of nanoparticles. Then, prepared samples were exposed to CO gas flue to study their gas sensor application using the localized surface plasmon resonance method. Increasing the Ni layer over Cu one shows an interesting result in both morphology and gas sensing sides. Advanced stereometric analyses for the surface topography of thin films in conjunction with Rutherford backscattering spectrometry and Spectroscopic analysis make a unique study in the field.
Tinnitus Affects Endogenous But Not Exogenous Auditory Attention Orienting
Authors: Yuan Yichen , Cai Yuexin , Wu Jiashuang , Li Jiahong , Huang Xiayin , Chen Guisheng , Lan Liping , Huang Hemei , Dong Hanwen , Zheng Yiqing , Yue Zhenzhu 
Published: July 2022

Purpose:
Previous studies have demonstrated that people with tinnitus show attention dysfunctions. In this study, we investigated the influence of tinnitus on attention orienting, especially whether the ability of attention orienting could be modulated by the degree of tinnitus.

Method:
Fifty-nine and 54 unilateral tinnitus participants were included in Experiment 1 and Experiment 2, respectively. All participants reported subjective tinnitus for at least 3 months and were divided into a mild tinnitus group (Tinnitus Handicap Inventory [THI] < 37) or a moderate to severe tinnitus group (THI ≥ 37) according to the THI score. An auditory exogenous attention task and an auditory endogenous attention task were adopted. In the exogenous task, a target sound following a cue sound was presented on either the left or right side. Participants were required to discriminate whether the target was pure tone or white noise. In the endogenous task, participants were required to pay attention to the stimuli on one side and judge the pitch of a target sound. Mixed-design analyses of variance were conducted for the mean reaction times and accuracy across the experimental conditions.

Results:
Our results showed that in the endogenous attention task, compared with the mild tinnitus group, moderate to severe tinnitus participants had better performance for stimuli presented on the tinnitus side but not on the nontinnitus side. In contrast, in the exogenous attention task, no differences were found between mild and moderate to severe tinnitus groups.

Conclusion:
The results suggest that the degree of tinnitus influences the performance of auditory endogenous attention but not auditory exogenous attention orienting.
Sentence Repetition Performance Differences in Bilingual and Monolingual Children
Authors: Friesen Deanna C. , Ward Olivia , Archibald Lisa M. D. 
Published: July 2022

Purpose:
This study examined language group differences in English syntactic knowledge based on performance on a sentence repetition task.

Method:

Fourth and sixth grade students who were monolinguals (
n
= 30), early bilinguals (i.e., simultaneous;
n
= 27), or late bilinguals (i.e., sequential;
n
= 29) completed an English sentence repetition task. Their responses were analyzed as a function of sentence length (short vs. long), sentence type (active vs. passive), phrase type (noun, verb, and prepositional), and word type (content vs. function).

Results:
Overall, early bilinguals' performance did not differ significantly from that of the monolinguals. However, these bilinguals recalled significantly more content words than function words on the long sentences. At each level of analysis, the late bilinguals' performance was less accurate than the other groups. The magnitude of these group differences was larger for passive sentences and prepositional phrases.

Conclusion:
Findings highlight areas of syntactic development that differ among groups and should be targeted for additional instruction with English language learners in elementary school.
Self‐sustained low‐frequency oscillation and its suppression in a practical AC/DC distribution network
Authors: Liu Wenkai , Zhan Meng 
Published: July 2022

AbstractA variety of power electronic equipment in AC/DC distribution networks causes oscillations with unknown origin. A self‐sustained low‐frequency oscillation appeared recently in a practical AC/DC distribution network in Tongli, Jiangsu Province, China. In this paper, the accident is introduced, and the oscillation phenomenon has been successfully reproduced by theoretical modelling. Based on the small signal model, the oscillation is well analysed and an additional controller is designed to suppress this low‐frequency oscillation. The reason of the oscillation is related to the power and control parameters of buck converter; the system is critically stable within the linear system stability theory, namely, the leading eigenvalues of the small‐signal system are on the imaginary axis. Meanwhile, a novel inhomogeneity phenomenon for oscillation amplitudes on different voltage‐level DC buses, which are not proportional to their steady‐state voltages, is found. This paper provides a new case study of self‐sustained oscillations coming from a practical AC/DC distribution network and is of great significance for our understanding of oscillations in electric power engineering.