bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum Nitric Oxide Metabolites (Nox) for Prognosis of Cardiovascular Death and Acute Myocardial Infarction in An ESSE-RF Case-Control Cohort with 6.5 Year Follow Up
Authors: Gumanova Nadezhda G. , Bogdanova Natalya L. , Metelskaya Victoria A. , Tarasov Vladimir I. , Kots Alexander Ya. , Kontsevaya Anna V. , Kutsenko Vladimir , Drapkina Oxana M. 
Published: March 2022

Abstract
Aims. The goal of the present case-control study was to assess associations of a panel of routine and experimental biomarkers with the risk for cardiovascular death and acute myocardial infarction (AMI) in a cohort recruited from the multicenter study “Cardiovascular Epidemiology in Russian Federation” (ESSE-RF).Methods and Results. A total of 222 subjects included cardiovascular death (N=48) and AMI cases (N=63) detected during 6.5-year follow up with corresponding numbers of matched healthy controls. The levels of 15 routine and experimental biochemical markers were assayed to analyze associations with the outcomes using logistic and Cox proportional hazard regressions validated by comparison between various groups to assess the estimated hazard ratio (eHR). Elevated levels of cardiac troponin I (cTnI), C-reactive protein (CRP), and metabolites of nitric oxide (NOx) were independently associated (P<0.001) with a higher risk of cardiovascular death in various models with adjusted eHRs ranging from 1.83 to 3.74. Elevated levels of NOx and cTnI were significantly (P<0.001) and independently associated with a higher risk of nonfatal AMI with eHR ranging from 1.78 to 2.67. Elevated levels of angiopoietin-like protein 3 (ANGPTL3) were significantly (P<0.001) and independently associated with a lower risk of cardiovascular death in certain models (eHRs from 0.09 to 0.16) and a higher risk of nonfatal AMI (eHR 2.07; P=0.01).Conclusion. Subsequent large scale validation studies should focus on the investigation of predictive impact of cTnI, NOx, CRP, and ANGPTL3 for the development of nationwide recommendations for individual baseline stratification of patients with potential cardiovascular risks.
Parkinson's Disease With Mild Cognitive Impairment May Has A Lower Risk Of Cognitive Decline After Subthalamic Nucleus Deep Brain Stimulation: A Retrospective Cohort Study
Authors: xie hutao , Bai Yutong , Jiang Yin , Zhang Jianguo 
Published: March 2022

Abstract
Objective: The cognitive outcomes induced by subthalamic nucleus deep brain stimulation (STN-DBS) remain unclear, especially in PD patients with mild cognitive impairment (MCI). This study explored the cognitive effects of STN-DBS in PD patients with MCI.Methods: This was a retrospective cohort study that included 126 PD patients who underwent STN-DBS; all patients completed cognitive and motor assessments before and at least 6 months after surgery. Cognitive changes were mainly evaluated by the Montreal cognitive assessment (MoCA) scale and the seven specific MoCA domains, including visuospatial, executive function, naming, attention, language, abstract, delayed recall, and orientation. Motor improvement was evaluated by the UPDRS-III. Cognitive changes and motor improvements were compared between PD-MCI and normal cognitive (NC) patients. Logistic regression analyses were performed to explore predictors of postoperative cognitive change. Results: At the time of surgery, 61.90% of the included PD patients had MCI. Compared with the PD-MCI group, the PD-NC group had a significantly higher proportion of cases with postoperative cognitive decline during follow-up of up to 36 months (mean 17.34 ± 10.61 months), including in global cognitive function, attention, memory, visuospatial, and executive function. Covariate-adjusted binary logistic regression analyses showed that preoperative global cognitive status was an independent variable for postoperative cognitive decline. We also found that preoperative cognitive specific function could predict its own decline after STN-DBS, except for the naming and orientation domains.Conclusion: PD-MCI patients are at a lower risk of cognitive decline after STN-DBS compared with PD-NC patients before surgery.
Evaluation of the Global Lung Function Initiative 2012 Reference Values for Spirometry in an Iranian Population
Authors: Sahebi Leyla , Rahimi Besharat , Shariat Mamak , Mousavy Seyyed Hosein 
Published: March 2022

Abstract
Background
Spirometry is an important measurement in detecting and monitoring of chronic obstructive pulmonary disease. The validity of the multi-ethnic GLI-2012 spirometric norms has been debated in some countries.
Aim
The aim of the present study was to evaluate the applicability of the GLI reference norms in the Iranian population.
Method
A cross-sectional study was performed on 622 healthy non-smoker population (204 males and 418 females, age range: 4 ± 82 years) between July 16 and August 27, 2019 in Iran. Z-scores for spirometric data (FEV1 (forced expiratory volume in one second), FVC (forced vital capacity) FEV1/FVC, and FEF25 − 75% (Forced expiratory flow averaged over the middle portion of FVC) were calculated. According to the agreement approved, a mean Z-score outside the range of ± 0.5 was considered as clinically significant.
Result
The mean (SD) Z-score values of FEV1, FVC, FEV1/FVC and FEF25 − 75% were 0.44(1.21), 0.49(1.14), 0.11(1.03), and − 1.13(0.99) in males and 0.61(1.14), 0.89(1.26), 0.17(0.88) and-0.49 (0.96) in females, respectively. The Z-score of FEV1/FVC was below the Lower Limit of Normal (LLN) in 3.43% of men and 2.01% of women (in ≥ 21 years), while these values were significantly higher in people under 21 years old.
Conclusion
The GLI reference values are not perfect for the Iranian population, especially in children below 10 years old and women. The use of the GLI reference values was appropriate in population above 21 years; however, they would overestimate the prevalence of airway obstruction in individuals below 21 years.
Impact of Aqueous Micellar Media on Biocatalytic Transformations Involving Transaminase (ATA); Applications to Chemoenzymatic Catalysis
Authors: Dussart-Gautheret Jade , Yu Julie , Ganesh Krithika , Rajendra Gaikwad , Gallou Fabrice , Lipshutz Bruce 
Published: March 2022

Abstract
Surfactant-enabled asymmetric ATA-catalyzed reductive aminations in aqueous buffered media are described, representative of the enhanced levels of conversion made possible by the presence of a nonionic surfactant in the water, thereby minimizing enzymatic inhibition and enabling 1-pot chemoenzymatic catalysis. Several applications are described highlighting these modified conditions that involve both biocatalysis and chemocatalysis that are environmentally responsible, indicative of the possibilities using chemistry in water. Also included herein is technology for converting a racemic benzylic alcohol to a nonracemic primary amine, and an especially efficient synthesis of the pharmaceutical (S)-rivastigmine.
Development Of A Detection System For ESR1 Mutations In Circulating Tumour DNA Using PNA-LNA-Mediated PCR Clamping
Authors: Kojima Yuki , Noguchi Emi , Yoshino Tomomi , Yagishita Shigehiro , Yazaki Shu , Okuma Hitomi S , Nishikawa Tadaaki , Tanioka Maki , Sudo Kazuki , Shimoi Tatsunori , Kazama Ayaka , Terasaki Hiroshi , Asano Sachiro , Fujiwara Yasuhiro , Hamada Akinobu , Tamura Kenji , Yonemori Kan 
Published: March 2022

Abstract
Background: ESR1 gene mutations are an essential mechanism of resistance to endocrine therapy in patients with oestrogen receptor-positive breast cancer. ESR1 mutations can exist at multiple sites and are typically detected polyclonally after endocrine therapy. The use of circulating tumour DNA (ctDNA) is a less invasive and less expensive method for assessing ESR1 mutations. Adequate amounts of DNA are required to assess polyclonal ESR1 mutations using next-generation sequencing (NGS). The aim of this study was to develop a method to assess polyclonal ESR1 mutations in blood samples.Methods: We developed a novel detection system to screen for ESR1-activated mutations in ctDNA by combining a peptide nucleic acid and locked nucleic acid polymerase chain reaction (PNA-LNA PCR) clamping assay for detection of the most common mutations: E380Q, Y537S, and D538G. Other mutations in codons 536-538 were detected by direct sequencing of the amplified product following clamp PCR. A validation assay was prospectively performed on clinical samples and compared with the NGS results. Results: The PNA-LNA PCR clamp assay was validated using six blood samples in which ESR1 mutations were detected by NGS and four samples in which no mutations were detected. Results of the PNA-LNA assay were comparable with those of NGS. We prospectively assessed the concordance between the PNA-LNA PCR clamp method and NGS. Using the PNA-LNA PCR clamp method, ESR1 mutations were detected in 5 of 18 samples, including those in which mutations were not detected by NGS due to the small amount of ctDNA.Conclusions: The PNA-LNA PCR clamping method is a highly sensitive and minimally invasive assay for the detection of polyclonal ESR1 mutations in the ctDNA of patients with breast cancer.
Expansion of Fibroblast Cell Sheets Using a Modified MEEK Micrografting Technique for Wound Healing Applications
Authors: Benchaprathanphorn Kanokaon , Sakulaue Phongphot , Siriwatwechakul Wanwipa , Muangman Pornprom , Chinaroonchai Kusuma , Namviriyachote Nantaporn , Viravaidya-Pasuwat Kwanchanok 
Published: March 2022

Abstract
One of many challenges in wound treatment is to provide sufficient wound area coverage to protect against pathogens and speed up the wound healing process. Recently, cell sheet engineering has shown promise in treating deep wounds. Unfortunately, the cell sheet size is too small for practical use. To overcome this limitation, the MEEK micrografting technique, currently being used in the treatment of extensive deep burns, was investigated. This technique was modified and applied to enlarge dermal fibroblast cell sheets, constructed using temperature-responsive PNIAM-co-AM graft copolymer, by transforming the intact cell sheets into small cell islands at expansion ratios of 1:3, 1:6 and 1:9. Afterwards, cell mechanisms essential for wound healing, including reattachment, proliferation, and migration were investigated. The fibroblast cells on MEEK gauzes possessed high cell viability, capable of reattachment and migration. The optimal expansion ratio having the highest migration rate was 1:6, possibly due to an effective intercellular communication distance. Therefore, the combination of cell sheet engineering with the MEEK micrografting technique could provide high quality cells with a large coverage area, which would be particularly beneficial in wound care applications.
Research on Modified High-performance Cement Mortar of Prefabricated Buildings Based on Orthogonal Test
Authors: Yuan Chengbin , Cai Yu , Gu Qiang , Sang Di 
Published: March 2022

Abstract
In order to improve the construction technology level of the connection nodes of prefabricated hydraulic structures, and improve the mechanical properties, fluidity and economy of the connection mortar, this paper adopts the orthogonal experimental design to improve the cement mortar by adding polymer dispersible polymer powder. sex. A modified high-performance mortar with a compressive strength of 10 MPa, a fluidity of 90 mm and a final compressive strength grade of M25 was studied. The factors and levels of the orthogonal test are: mortar ratio 1:3、1:4、1:5; silica powder content of 4%、6%、8%; dispersible polymer powder content of 3%、5%、7%. After research, the optimal mixing ratio of modified high-performance mortar is 1:3, the content of silicon powder is 6%, the content of redispersible latex powder is 3%, the content of early strength water reducing agent is 0.1%, and the content of defoamer is 0.5%. The experimental verification is also carried out. This new type of modified high-performance cement mortar provides a new idea for the connection material of prefabricated hydraulic structures, which is of great significance for improving the integrity of prefabricated hydraulic structures and the durability of the connection.
Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame
Authors: Wakao Shohei , Oguma Yo , Kushida Yoshihiro , Kuroda Yasumasa , Tatsumi Kazuki , Dezawa Mari 
Published: March 2022

Abstract
Stem cells undergo cytokine-driven differentiation; however, this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing ‘model cells’ (apoptotic differentiated cells) was found to only require a short time frame. Pluripotent-like Muse cells, multipotent mesenchymal stem cells, and neural stem cells phagocytosed apoptotic differentiated cells via different phagocytic receptor subsets than macrophages. The phagocytosed-differentiated cellderived contents (e.g. transcription factors) were quickly released into the cytoplasm, translocated into the nucleus and bound to promoter regions of the stem cell genomes. Within 24~36 h, the cells expressed lineage-specific markers corresponding to the phagocytosed-differentiated cells, both in vitro and in vivo . At one week, the gene expression profiles were similar to those of the authentic differentiated cells and expressed functional markers. Differentiation was limited to the inherent potential of each cell line: triploblastic-, adipogenic-/chondrogenic-, and neural-lineages, respectively. Disruption of phagocytosis, either by phagocytic receptor inhibition via small interfering RNA or Annexin V treatment, impeded differentiation in vitro and in vivo . Collectively, our study uncovers a simple way to directly transfer differentiation-directing factors to trigger rapid differentiation of somatic stem cells into the target cell lineage.
Occurrence And Seasonal Disparity Of Emerging Endocrine Disrupting Chemicals In A Drinking Water Supply System And Associated Health Risk
Authors: Kumawat Manoj , Sharma Poonam , Pal Namrata , James Meenu Mariya , Verma Vinod , Tiwari Rajnarayan R , Shubham Swasti , Sarma Devojit Kumar , Kumar Manoj 
Published: March 2022

Abstract
Contamination of drinking water with endocrine-disrupting chemicals (EDCs) raisesconcerns over the security and long-term sustainability of clean water supplies as well ashuman exposure via daily water intake. In this study, the seasonal disparity and occurrence ofsix phthalates and bisphenol A in the drinking water supply system and associated health risk wereexamined. DEHP was the most prevalent phthalate esters congener ranging from 1.14 - 8351.85 µg/L(winter) and 0.552 - 410.29 µg/L (summer) surpassing the permissible limit.Meanwhile, BPA concentrations were found under the permissible limit. The results suggested that PAEsconcentration displayed significant seasonal variations with the highest in winter and thelowest in the summer.The exposure toPAEs and BPA from drinking water was assessed, and the results indicated a possible healthrisk to humans with a Hazard Quotient (HQ) for DEHP.The findings necessitate an immediate scrutiny of these EDCs indrinking water supply system and are critical for implementing effective technologies at theWTP scale to ensure the quality and safety of drinking water in order to ascertain human andenvironmental health.
Mechanical Stress Protects Against Chondrocyte Pyroptosis through Lipoxin A4 via Synovial Macrophage M2 Subtype Polarization in an Osteoarthritis Model
Authors: Shen Peng , Jia Shuangshuo , Wang Yang , Zhou Xiaonan , Zhang Delong , Jin Zhuangzhuang , Wang Ziyuan , Liu Donghao , Bai Lunhao , Yang Yue 
Published: March 2022

Abstract
Our previous study found that lipoxin A4 (LXA4) exerted therapeutic effects on osteoarthritis (OA). In this study, we evaluated the effects of LXA4 via synovial macrophage M1/M2 subtype polarization on chondrocyte pyroptosis and mechanisms during mechanical stimulation. Synovial macrophages were subjected to various LXA4 concentrations and cyclic tensile strain (CTS) conditions to determine optimal co-culture conditions. The effects of LXA4 on chondrocyte pyroptosis, as represented by macrophage M1/M2 subtype polarization, were detected by western blot, immunofluorescence, and flow cytometry analyses. Forty male Sprague-Dawley rats were randomly divided into four groups (n = 10): control group (CG), OA group (OAG), OA with moderate-intensity treadmill exercise (OAE), and OAE + BOC-2 (an LXA4 antagonist). All rats were evaluated using histology, enzyme-linked immunosorbent assay (ELISA), quantitative PCR, and western blot analyses. We found that LXA4 was downregulated in articular fluid and that CD 86/Arg 1 was up-regulated in the synovium of patients with increasing Kellgren-Lawrence grade. In vitro, CTS and LXA4 both promoted M2 subtype polarization of synovial macrophages, inhibiting the nuclear translocation of NF-κB p65 and the NLRP3 formation in chondrocytes. In vivo, the OAE treatment exerted protective effects on articular cartilage and facilitated M2 polarization of synovial macrophages. These effects were suppressed by BOC-2 treatment. We concluded that moderate CTS enhances the therapeutic effects of LXA4 by inhibiting the nuclear translocation of NF-κB p65 and NLRP3. Furthermore, the therapeutic effects of LXA4 during treadmill exercise in monoiodoacetate-induced OA were driven by promotion of synovial macrophage M2 subtype polarization.
Comprehensive Pan-Cancer Analysis of 33 Human Cancers Reveals Immunotherapeutic Value of FAK/PTK2
Authors: Shi Yujing , Ju Mengyang , Xue Ning , Zhang Yumeng , Liang Liang , Sun Xinchen , Di Xiaoke 
Published: March 2022

Abstract
Background: The immune environment in tumors is the key factor affecting the survival and immunotherapeutic response of patients.This research aimed to explore the underlying association between FAK/PTK2 and cancer immunotherapy in 33 human cancers.Methods: Gene expression data and clinical features of 33 cancers were retrieved from the Cancer Genome Atlas Database. The immunotherapy cohorts included GSE 67501, GSE78220 and IMVIGOR210, which were derived from the comprehensive gene expression database or from previous studies. Clinical parameters including patient age, gender, survival rate and tumor stage were analyzed to evaluate the prognostic value of FAK/PTK2. FAK/PTK2 activity was detected by single-sample gene set enrichment analysis and used to compare the difference between FAK/PTK2 transcriptome and protein expression levels. To better understand the role of FAK/PTK2 in cancer immunotherapy, we analyzed its correlations with tumor microenvironment and with immune processes / elements (e.g. immune cell infiltration, immunosuppressants and stimulants) and major histocompatible complexes. Potential pathways associated with FAK/PTK2 signaling in cancers were also explored. Correlations between FAK/PTK2 and two immunotherapeutic biomarkers (tumor mutation load and microsatellite instability) were studied. Finally, the three independent immunotherapy cohorts were used to study the relationship between FAK / PTK2 and immunotherapeutic response.Results: Although FAK/PTK2 is not closely associated with age (13/33), gender (5/33) or tumor stage (5/33) in any of the studied human cancers, it has potential prognostic value for predicting patient survival. Consistency between FAK/PTK2 activity and expression exists in some cancers (3/33). Generally, FAK/PTK2 is robustly correlated with immune cell infiltration, immune modulators, and immunotherapeutic markers. Moreover, high FAK/PTK2 expression is significantly related to immune-relevant pathways. However, FAK/PTK2 is not significantly correlated with the immunotherapeutic response.Conclusions: Research on the immunotherapeutic value of FAK/PTK2 in 33 human cancers provides evidence regarding the function of FAK/PTK2 and its role in clinical treatment. However, given the use of a bioinformatics approach, our results are preliminary and require further validation.
Laboratory breeding of Phortica spp. (Diptera: Drosophilidae), vectors of the zoonotic eyeworm Thelazia callipaeda
Authors: Bernardini Ilaria , Poggi Cristiana , Manzi Sara , Bezerra-Santos Marcos Antonio , Beugnet Frédéric , Fourie Josephus , Otranto Domenico , Pombi Marco 
Published: March 2022

Abstract
Background - The establishment of arthropod colonies is an important tool to manipulate disease vectors, investigate their life history traits, and their vector competence, prevention of vector borne pathogen transmission, or to assess insecticidal/repellent activities. Some species of drosophilid flies belonging to the genus Phortica feed on ocular secretions of mammals, acting as biological vectors of the zoonotic eyeworm Thelazia callipaeda. This study describes an effective breeding protocol of Phortica variegata and Phortica oldenbergi in insectary conditions. Materials and methods - Gravid flies of P. oldenbergi, P. variegata and Phortica semivirgo were field collected in wooded areas of Lazio region (Italy) and allowed to oviposit singularly to obtain isofamilies. Flies were maintained in ovipots (200ml) with a plaster-covered bottom to maintain high humidity level inside. Adult feeding was guaranteed by freshly apple and a liquid dietary supplement containing NaCl and mucin proteins, while larval development was obtained by Drosophila-like agar feeding medium. The breeding performances of two media were compared: a standard one based on cornmeal flour and an enriched medium based on chestnut flour. All conditions were kept in a climatic chamber with a photoperiod of 14:10h light:dark, 26±2°C and 80±10% RH. Results - From a total of 130 field collected Phortica spp. three generations (i.e., 783 F1, 109 F2, 6 F3) were obtained. Phortica oldenbergi was the species with higher breeding performance, being the only species reaching F3. Chestnut-based feeding medium allowed higher adult production and survival probability in both P. oldenbergi and P. variegata. Adult production/female was promising in both species (P. oldenbergi: 13.5 F1/f; P. variegata: 4.5 F1/f), indicating the possibility to obtain stable colonies.Conclusions - This standardized breeding protocol, based on controlled climatic parameters and fly densities, together with the introduction of an enriched feeding medium, allowed to investigate aspects of life history traits of Phortica spp. involved in the transmission of T. callipaeda. Obtaining F3 generation of these species for the first time paved the road for the establishment of stable colonies, an essential requirement for future studies on these vectors in controlled conditions.
Heat and mass transfer in binary fluids containing alcohols with the Soret effect
Authors: Almasi Mohammad 
Published: March 2022

Abstract
In this study, heat and mass transfer in binary systems containing methylcyclohexane and n-alkanol (1-pentanol, 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol, and 1-decanol) at 293.15 K to 323.15 K was investigated considering the mutual diffusion coefficient, thermal diffusion coefficient, Soret effect, and the separation ratio. Soret coefficient \({S_T}\)for methylcyclohexane is positive and indicates that this molecule is enriched in the colder regions of the liquids. Obtained results demonstrate that increasing the alkyl chain length reduces its tendency to move to the warmer regions. This behavior means that molecular forces are reinforced with the alcohol chain length. The new method's advantages over previous methods for calculating the thermal behavior of the mixtures are discussed.