bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimating the Shear-Wave Velocities of Shallow Sediments in the Yellow Sea Using Ocean-Bottom-Seismometer Multicomponent Scholte-Wave Data
Authors: Wang Yuan , You Qingyu , Hao Tianyao 
Published: January 2022

Scholte-wave dispersion analysis is effective at imaging the relatively low shear-wave velocity of shallow marine sediments in marginal seas. The combination of a four-component ocean-bottom-seismometer (OBS) and a towed air-gun source can economically and effectively acquire the marine dispersive seismic data. Extracting higher-order dispersive Scholte wave modes is the most critical problem in the dispersion analysis method. The extremely low shear-wave velocity and severe attenuation in the top hundreds of meters of marginal sea sediment provide an uneven dispersive energy distribution for the four components of the Scholte wave data. The fundamental mode dispersive energy dominates in the vertical component and higher-order modes dominate in the horizontal component. We developed the method of the four-component OBS Scholte velocity-spectra stacking, which can effectively, rapidly, and robustly extract higher-order modes. We imaged the shear-wave velocity structure of complicated shallow marine sediment in the North Yellow Sea using an active OBS seismic profile with a large-volume air-gun array. The fourth higher-order Scholte wave mode can be imaged with the four-component velocity-spectra stacking method with a lower frequency range of 1.0–7.0 Hz. Only the second-order mode can be recognized from the dispersion energy image of the single vertical component. The joint inversion of multimode dispersion curves can provide more accuracy and deeper constraints for the inverted model; thus, the constraint depth with five modes increases by a factor of 1.9 compared with single fundamental mode inversion. The inverted profile suggests a low shear-wave velocity of 123–670 m/s and strong lateral variations within 350 m. The main regional geological structures are shown by the inverted shear-wave velocity structure.
The Effects of Endometrial Thickness on Pregnancy Outcomes of Fresh IVF/ICSI Embryo Transfer Cycles: An Analysis of Over 40,000 Cycles Among Five Reproductive Centers in China
Authors: Xu Jianing , Zhang Shaodi , Jin Lei , Mao Yundong , Shi Juanzi , Huang Rui , Han Xiao , Liang Xiaoyan , Zhang Cuilian 
Published: January 2022

ObjectiveTo investigate the effects of endometrial thickness (EMT) on pregnancy outcomes on hCG trigger day in fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles.MethodsA total of 42,132 fresh cycles between 1 January 2013 and 31 December 2019 were included in this retrospective cohort study. Data were collected from five reproductive centers of large academic or university hospitals in China. All patients were divided into different groups according to their endometrial thickness on hCG trigger day. Multivariate regression analysis, curve fitting and threshold effect analysis were performed.ResultsAfter adjusting for age, body mass index, infertility type, number of embryos transferred, number of retrieved oocytes and COS (controlled ovarian stimulation) protocols, significant associations were found between endometrial thickness and clinical pregnancy rate (adjusted odds ratio [aOR]: 1.05; 95% confidence interval [CI]: 1.06–1.08, P &lt; 0.0001), live birth rate (aOR: 1.04; 95% CI: 1.03–1.05, P &lt; 0.0001) as well as miscarriage rate(aOR: 0.96; 95% CI: 0.94 – 0.98, P &lt; 0.0001). When the endometrial thickness was less than 12mm, the clinical pregnancy rate and live birth rate were increased significantly by 10% and 9%(OR:1.10; 95%CI: 1.08-1.12, OR:1.09; 95%CI: 1.07-1.11), respectively, along with the increase of each millimeter increment of endometrial thickness. However, when the EMT ranged from 12-15 mm, were stable at the ideal level, that were not significantly associated with EMT growth. Additionally, clinical pregnancy rate and live birth rate were slightly reduced by 6% and 4% when EMT was ≥15mm. Meanwhile, the miscarriage rate was significantly declined by 8% (OR:0.92; 95%CI: 0.90-0.95)with each millimeter increment of EMT. And when EMT was thicker than 12mm, the miscarriage rate didn’t change any more significantly.ConclusionsEndometrial thickness exhibits a curvilinear relationship with pregnancy outcomes in fresh embryo transfer cycles. Clinical pregnancy rate, live birth rate and miscarriage rate may achieve their optimal level when EMT ≥ 12 mm, but some adverse pregnancy outcomes would be observed when EMT ≥15 mm especially for clinical pregnancy.
Intracranial Germinoma Misdiagnosed as Hyperthyroidism: A Case Report and Review of the Literature
Authors: Tian Juan , Wu Jialu , Yan Zhe , Huang Hui 
Published: January 2022

Intracranial germ cell tumors (GCTs) are relatively rare, which account for 0.5% of all primary intracranial neoplasms. Intracranial germinomas most commonly occur in the pineal and suprasellar region, making up the majority of all intracranial GCTs. For its diversified clinical manifestations, the diagnosis is easily confused with other diseases. Here, we present a case of a 19-year-old boy with intracranial germinoma who was preliminarily misdiagnosed as hyperthyroidism for the symptoms of weight loss and thyroid dysfunction.
ANGPTL4 Expression in Ovarian Granulosa Cells Is Associated With Polycystic Ovary Syndrome
Authors: Jiang Qi , Pan Ye , Li Ping , Zheng Yanjun , Bian Yuehong , Wang Wenqi , Wu Guihua , Song Tian , Shi Yuhua 
Published: January 2022

ObjectivesThis study aims to characterize the expression of ANGPTL4 in ovarian granulosa cells (GCs) and its association with polycystic ovary syndrome (PCOS).MethodsThis study included 104 PCOS patients and 112 women in control group undergoing in vitro fertilization-embryo transfer (IVF-ET) from the reproductive hospital affiliated with Shandong University from 2019 to 2021. By reverse transcription and real-time quantitative (RT-q) PCR, the mRNA expression of ANGPTL4 in GCs was assessed, and clinical information for these patients were then reviewed and analyzed.ResultsThe RT-qPCR results showed that ANGPTL4 expression in the control group was significantly lower than that in the PCOS group (p = 0.000) and had positive association with AMH (r = 0.211), HOMA-IR (r = 0.174), LDL/HDL (r = 0.176), ApoB/ApoAI (r = 0.155), and TC/HDL (r = 0.189). Additionally, the high expression of ANGPTL4 in the ovarian granulosa cells might be an independent predictor in PCOS (OR: 3.345; 95% CI: 1.951–5.734) with a close contact with incidence of PCOS (AUC: 0.704; 95% CI: 0.633–0.774, p &lt; 0.001).ConclusionsOur study revealed higher ANGPTL4 expression in ovarian GCs with PCOS. Its association with glucose and lipid metabolism showed that ANGPTL4 might play an important role in PCOS metabolism and pathogenesis.
Ultrasound and Contrast-Enhanced Ultrasound Characteristics Associated With cN1 and Microscopic pN1 in Papillary Thyroid Carcinoma
Authors: Li Wen , Qiu Shusheng , Ren Ling , Li Qiuyang , Xue Shaowei , Li Jie , Zhang Yan , Luo Yukun 
Published: January 2022

ObjectivesLymph node metastases (LNMs) could be stratified into clinical N1 (cN1) and microscopic pN1 (pathological N1), which bear different biological behavior and prognosis. Our study aimed to investigate the associations between LNMs and primary tumor’s US (ultrasound) and CEUS (contrast-enhanced ultrasound) characteristics based on the stratification of LNMs into cN1 and microscopic pN1 in papillary thyroid carcinoma (PTC).MethodsFrom August 2019 to May 2020, 444 consecutive PTC patients who underwent preoperative neck US and CEUS evaluation were included. According to regional lymph node status, the patients were classified into cN1 group versus cN0 (clinical N0) group and microscopic pN1 group versus pN0 (pathological N0) group. For multiple PTCs, the largest one was selected for the evaluation of US, CEUS and clinical features. Univariate and multivariate analyses were performed to determine independent predictors of cN1 and microscopic pN1.Results85 cN1 versus 359 cN0 patients and 117 microscopic pN1 versus 242 pN0 patients were analyzed. Multivariate logistic regression analysis showed that &lt;55-years-old (OR: 2.56 (1.08–6.04), male [OR: 2.18 (1.22–3.91)], large size [OR: 2.59 (1.71–3.92)], calcification [OR: 3.88 (1.58–9.51)], and hyper-enhancement [OR: 2.78 (1.22–6.30)] were independent risk factors of cN1, while &lt;55-years-old [OR: 1.91 (1.04–3.51)], large size [OR: 1.56 (1.003–2.42)], multifocality [OR: 1.67 (1.04–2.66)] were independent risk factors of microscopic pN1.ConclusionsFor patients with PTC, young age, male, large size, calcification, and hyper-enhancement were independent predictors of cN1, while young age, large size and multifocality were independent predictors of microscopic pN1.
Migraine Is More Prevalent in Advanced-Stage Endometriosis, Especially When Co-Occuring with Adenomoysis
Authors: Wu Yingchen , Wang Hao , Chen Shengfu , Lin Yueming , Xie Xiaoqian , Zhong Guangzheng , Zhang Qingxue 
Published: January 2022

BackgroundEmerging data suggest a significant association between migraine and endometriosis, however the relationship between migraine and endometriosis severity or adenomyosis is unclear. Our objectives were to explore the relationship between migraine and endometriosis, according to the endometriosis severity and co-exist with adenomyosis or not.MethodsThis case-control study of 167 endometriosis patients verified by surgery and 190 patients for other benign gynecological conditions (control subjects) was performed from September 2017 and January 2021. There is 49 adenomyosis detected by transvaginal ultrasound or histologic diagnosis among the endometriosis patients. Besides, we also included 41 adenomyosis but without endometriosis patients as a subgroup. All women completed a self-administered headache questionnaire and diagnosed as migraine according to the International Headache Society classification. The severity and stage of endometriosis was evaluated with revised American Society of Reproductive Medicine (rASRM) score. We used logistic regression to estimate the association between the presence of migraine and endometriosis severity while accounting for important confounders, including age, body mass index (BMI) and family history of migraine. We also estimate the risk of adenomyosis alone and adenomyosis with co-occurring endometriosis in migrainous women.ResultsMigraine was significantly more prevalent in endometriosis patients compared with controls (29.9% vs. 12.1%, p&lt;0.05), but the prevalence was similar between isolated adenomyosis patients and controls (9.8% vs.12.1%, p&gt;0.05). For all endometriosis and control participants, migraineurs were 4.6-times (OR=4.6; 95% CI 2.7-8.1) more likely to have severe endometriosis. However, the strength of the association decreased when the analysis examined in moderate stage (OR=3.6, 95% CI 2.1-6.2). The risk of mild and minimal endometriosis was not significant (OR=1.9, 95%CI 0.9-4.0; OR=1.6, 95% CI 0.8-3.4; respectively). When we divided the endometriosis patients according to whether co-occurring with adenomyosis. We found in migrainous women, the risk of endometriosis co-exist with adenomyosis increased, with nearly fivefold greater odds compared with control (OR=5.4;95% CI 3.0-9.5), and nearly two times higher than the risk of endometriosis without co-exist adenomyosis patients (OR=2.2; 95% CI 1.2-3.8).ConclusionOur study supports the strong association between migraine and endometriosis. We found migrainous women suffer more frequently from sever endometriosis, especially endometriosis with co-occurring adenomyosis. It is advisable to heighten suspicion for patients who presenting with either these conditions in order to optimize therapy.
Social Science Applications in Sustainable Aviation Biofuels Research: Opportunities, Challenges, and Advancements
Authors: Anderson Brian J. , Mueller Daniel W. , Hoard Season A. , Sanders Christina M. , Rijkhoff Sanne A. M. 
Published: January 2022

Social science has an important role in aviation biofuels research, yet social science methods and approaches tend to be underdeveloped and under-utilized in the broader aviation biofuels literature and biofuels overall. Over the last 5 years, social science approaches in aviation biofuels research, particularly site-selection, have made several advances. Where early site-selection models either entirely excluded social science concepts or included only a few measurements using poor proxies, current models more accurately, and more comprehensively capture key social science concepts to better examine and predict project implementation success and long-term sustainability. Despite several studies published within the last 20 years noting the need for more empirical studies of social sustainability and improvement in incorporation of social criteria, progress has remained rather stagnant in several areas. To help move the field forward, we conduct a review of the current state of social science research in aviation biofuels with a focus on sustainability, site-selection, and public acceptance research, identifying key approaches, important developments, and research gaps and weaknesses of current approaches. While several review studies already exist, they tend to focus on a single area of biofuels such as public acceptance. By broadening our review to several areas, we are able to identify several common limitations across these areas that contribute to the continued underutilization of social science approaches in aviation biofuels. This includes the preference for practical and reliable indicators for social criteria that prioritize quantitative methods over other approaches. Based on these limitations, we make several recommendations to improve social science research in aviation biofuels, including ensuring that social scientists are key members of the research team, the adoption of a mixed-methods research designs that combines quantitative and qualitative approaches that better measure some criteria and local-level impacts, and adequate resources for social science research throughout biofuel development projects as these methods are often more time-consuming and costly to implement. We argue that implementing these recommendations in future aviation biofuel development projects will improve social science approaches utilized in aviation biofuels research and address a long-acknowledged gap in the field.
Removal of Mercury, Cadmium, and Lead Ions by Penicillium sp.
Authors: Sánchez-Castellón Juan , Urango-Cárdenas Iván , Enamorado-Montes Germán , Burgos-Nuñez Saudith , Marrugo-Negrete José , Díez Sergi 
Published: January 2022

Discharge of metals into the environment generates significant impact upon human health and biological cycles. Some microorganisms such as fungi are known for their high metal adsorption capacity. The aim of this work was to evaluate the capacity for Penicillium sp. molds in the removal of Pb, Cd, and Hg from aqueous solutions by isolating the fungal strain from an artisanal gold mine soil. The biosorption experiments showed that optimum conditions for metal removal were noted at 150 min, acidic pH (4–5), 60 °C, and 2 g of biomass. The accomplished removal was 92.4% for Pb, 80% for Cd, and 99.6% for Hg, at a concentration of 51.5 mg/L. Kinetic analyses and isotherms best fit the pseudo–second-order and Langmüir models, respectively. Infrared spectra show functional groups (–OH, –NH, C-N, C-H, N-H, and C=O) that play an essential role in the adsorption of Pb, Hg, and Cd on fungal biomass.
LncRNA VPS9D1-AS1 Promotes Malignant Progression of Lung Adenocarcinoma by Targeting miRNA-30a-5p/KIF11 Axis
Authors: Liu Jiefeng , Feng Yuhua , Zeng Xinyu , He Miao , Gong Yujing , Liu Yiping 
Published: January 2022

Objective: This research probed into the molecular mechanisms of long non-coding RNA (lncRNA) VPS9D1 Antisense RNA 1 (VPS9D1-AS1) in lung adenocarcinoma (LUAD).Methods: lncRNA expression level was evaluated bioinformatically, and its downstream miRNA/mRNA regulatory axis was predicted by bioinformatics methods as well. qRT-PCR was used to measure VPS9D1-AS1, miRNA-30a-5p, and kinesin family member 11 (KIF11) expression. Western blot was performed to measure KIF11 protein expression. Proliferation, migration, and invasion of LUAD cells were all observed by cell biological function experiments. Dual-luciferase assay detected binding between miRNA-30a-5p and VPS9D1-AS1 or KIF11, respectively. RIP experiment detected interaction between VPS9D1-AS1 and miRNA-30a-5p.Results: VPS9D1-AS1 and KIF11 were increased in LUAD, whereas miRNA-30a-5p was decreased. VPS9D1-AS1 promoted the malignant progression of LUAD cells and could sponge miRNA-30a-5p. MiRNA-30a-5p could restore the impact of VPS9D1-AS1 on LUAD cells. KIF11 was a target downstream of miRNA-30a-5p. VPS9D1-AS1 could upregulate KIF11 expression through competitively sponging miRNA-30a-5p, and KIF11 could restore the impact of miRNA-30a-5p on LUAD cells.Conclusion: VPS9D1-AS1 could foster malignant progression of LUAD via regulating miRNA-30a-5p/KIF11 axis, suggesting that VPS9D1-AS1 is key to regulating the malignant progression of LUAD.
Gene Spectrum and Clinical Traits of Nine Patients With Oocyte Maturation Arrest
Authors: Huo Mingzhu , Zhang Yile , Shi Senlin , Shi Hao , Liu Yidong , Zhang Lingyun , Wang Yanchi , Niu Wenbin 
Published: January 2022

Background: Oocyte maturation arrest is a disease that produces immature oocytes and cannot be mature after culturing in vitro, which leads to female primary infertility. We aimed to summarize nine representative patients in our center to retrospectively analyze the genetic variants and clinical characteristics of oocyte maturation arrest.Methods: This study examined and analyzed nine families with oocyte maturation arrest. Whole-exome sequencing (WES) of the probands was performed to detect the pathogenic variants. Sanger sequencing verified the WES findings in patients and available parents. ExAC database was used to search the variant frequency. The variants were assessed by pathogenicity and conservational property prediction analysis and according to the American College of Medical Genetics and Genomics (ACMG). Phenotypes of oocytes were evaluated by a light microscopy, and the phenotype-genotype correlation was also evaluated.Results: Nine pathogenic variants in five genes were detected in nine patients, of which three were novel variants, including PATL2 [c.1374A &gt; G (p. Ile458Met)] and [1289-1291del TCC (p. Leu430del)] and ZP2 [c.1543C &gt; T (p. Pro515Ser)]. Nine variants were predicted to be pathogenic, resulting in different types of oocyte maturation arrest and clinical phenotypes.Conclusion: Three novel pathogenic variants were identified, enabling the expansion of the gene variant spectrum. The related pathogenic mutations of the PATL2, TUBB8, and ZP1∼3 genes were highly suggestive of being causative of oocyte maturation arrest.
Solitary Median Maxillary Central Incisor Syndrome: An Exploration of the Pathogenic Mechanism
Authors: Li Jie , Liu Dandan , Liu Yang , Zhang Chenying , Zheng Shuguo 
Published: January 2022

This study aimed to identify the genetic cause of one Chinese family with solitary median maxillary central incisor (SMMCI) and explore the relationship between genotype and its phenotype. One Chinese family with clinical diagnosis of SMMCI was collected. Single Nucleotide Polymorphism (SNP) array was performed and identified variation was confirmed by whole-genome sequencing (WGS). The reported chromosomal abnormalities and pathogenic genes in patients with SMMCI in literature were reviewed and summarized. The proband was an 8-year-old boy presenting a typical solitary median maxillary central incisor with a range of other phenotypic anomalies, including ptosis. SNP array revealed a 14.3 Mbp heterozygous deletion at chromosome 18p11.32-p11.21 in the proband but not in the unaffected parents. WGS further confirmed the identified deletion. 194 genes were involved in the chromosome region. Among them, 12 genes had been shown to be associated with diseases, including TGIF1, a reported SMMCI gene. The de novo 18p deletion resulted in SMMCI in the present study. Our results provide new genetic evidence that structural abnormality in chromosome 18p contributes to solitary median maxillary central incisor.
Development and Validation of a Pyroptosis-Related Signature for Predicting Prognosis in Hepatocellular Carcinoma
Authors: Ding Jianfeng , He Xiaobo , Luo Wei , Zhou Weiguo , Chen Rui , Cao Guodong , Chen Bo , Xiong Maoming 
Published: January 2022

Hepatocellular carcinoma (HCC) has emerged as a primary health problem and threat to global mortality, especially in China. Since pyroptosis as a new field for HCC prognosis is not well studied, it is important to open a specific prognostic model. In this study, consensus clustering method for 42 pyroptosis-related genes to classify 374 HCC patients in the TCGA database. After cox regression analysis of the differentially expressed genes between the two clusters, LASSO-Cox analysis was then performed to construct a pyroptosis-related prognostic model with 11 genes including MMP1, KPNA2, LPCAT1, NEIL3, CDCA8, SLC2A1, PSRC1, CBX2, HAVCR1, G6PD, MEX3A. The ICGC dataset was served as the validation cohort. Patients in the high-risk group had significantly lower overall survival (OS) rates than those in the low-risk group (p &lt; 0.05). COX regression analysis showed that our model could be used as an independent prognostic factor to predict prognosis of patients and was significantly correlated with clinicopathological characteristics. Nomogram showing the stability of the model predicting the 1, 3, 5 year survival probability of patients. In addition, based on the risk model, ssGSEA analysis revealed significant differences in the level of immune cell infiltration and activation of immune-related functional pathways between high and low-risk groups, and patients with the high-risk score may benefit more from treatment with immune checkpoint inhibitors. Furthermore, patients in the high-risk group were more tend to develop chemoresistance. Overall, we identified a novel pyroptosis-related risk signature for prognosis prediction in HCC patients and revealed the overall immune response intensity of the tumor microenvironment. All these findings make the pyroptosis signature shed light upon a latent therapeutic strategy aimed at the treatment and prevention of cancers.