bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pleural empyema caused by dropped gallstones after laparoscopic cholecystectomy for acute cholecystitis: a case report
Authors: Tokuda Aya , Maehira Hiromitsu , Iida Hiroya , Mori Haruki , Nitta Nobuhito , Maekawa Takeru , Takebayashi Katsushi , Kaida Sachiko , Miyake Toru , Kuroda Ryo , Yamamoto Haruka , Tani Masaji 
Published: April 2022

Abstract
Background
Dropped gallstones during laparoscopic cholecystectomy (LC) sometimes induce postoperative infectious complications. However, pleural empyema rarely occurs as a complication of LC.

Case presentation
We present the case of a 66-year-old woman with right pleural empyema. She previously underwent LC for acute gangrenous cholecystitis 11 months ago. The operative report revealed iatrogenic gallbladder perforation and stone spillage. The bacterial culture of the gallbladder bile was positive for Escherichia coli. Chest and abdominal computed tomography revealed right pleural effusion, perihepatic fluid collection, and multiple small radiopaque density masses. Although ultrasound-guided transthoracic drainage was performed, the drainage was incomplete, and systemic inflammatory reaction persisted. Consequently, thoracotomy and laparotomy with gallstone retrieval were performed, and the patient recovered completely. The patient has remained well without complications after 14 months of follow-up.

Conclusions
We report a rare case of pleural empyema caused by dropped gallstones after LC. This case emphasized the importance of completely retrieving the dropped gallstones to prevent late infectious complications after LC.
Does socioeconomic inequality exist in minimum acceptable diet intake among children aged 6–23 months in sub-Saharan Africa? Evidence from 33 sub-Saharan African countries’ demographic and health surveys from 2010 to 2020
Authors: Belay Daniel Gashaneh , Taddese Asefa Adimasu , Gelaye Kassahun Alemu 
Published: April 2022

AbstractBackgroundChild undernutrition is a major public health problem in many resource-poor communities in the world. More than two-thirds of malnutrition-related child deaths are associated with inappropriate feeding practices during the first 2 years of life. Socioeconomic inequalities are one of the most immediate determinants. Though sub-Saharan Africa (SSA) shares the huge burden of children undernutrition, as to our search of literature there is limited evidence on the pooled magnitude, socioeconomic inequalities of minimum acceptable diet intake and its contributing factors among children aged 6 to 23 months in the region. This study aimed to assess the level of socio-economic inequalities of minimum acceptable diet intake, and its contributor factors among children aged 6–23 months in SSA using recent 2010–2020 DHS data.MethodsA total of 78,542 weighted samples from Demographic and Health Survey datasets of SSA countries were used for this study. The data were cleaned using MS excel and extracted and analyzed using STATA V.16 software. The concentration index and curve and wag staff type decomposition analysis were applied to examine wealth-related inequalities in the outcomes.P-value < 0.05 was taken to declare statistical significance.ResultsThe pooled magnitude of MAD intake among children age 6–23 months in SSA was 9.89% [95%CI: 8.57, 11.21%] ranging from 3.10% in Guinea to 20.40% in Kenya. MAD intake in SSA was disproportionately concentrated on the rich households (pro-rich) [C = 0.191; 95% CI: 0.189, 0.193]. Residence (36.17%), media exposure (23.93%), and women’s education (11.63%) explained the pro-rich inequalities in MAD intake. The model explained 55.55% of the estimated socioeconomic inequality in MAD intake in SSA.Conclusion and recommendationsMinimum acceptable diet intake in SSA is relatively low. There are moderate socioeconomic inequalities in MAD intake in SSA, mainly explained by residence, media exposure and women’s education. The government of sub-Saharan African countries should plan and work in short terms through the program that endorses women empowerment such as income generation, cash assistance for mothers who have under 2 years of children and women employment using affirmative actions, and nutrition education such as media campaigns and promoting breast feedings. Long-term plans are also needed for those SSA countries with lower income status through programs to enhance their country’s economy to the middle and higher economic level and to improve the wealth index of individual households to narrow the poor-rich gap in the minimum acceptable diet intake.
Comparison of fluid dynamics changes due to physical activity in 3D printed patient specific coronary phantoms with the Windkessel equivalent model of coronary flow
Authors: Sommer Kelsey N. , Bhurwani Mohammad Mahdi Shiraz , Iyer Vijay , Ionita Ciprian N. 
Published: April 2022

Abstract
Background
3D printing (3DP) used to replicate the geometry of normal and abnormal vascular pathologies has been demonstrated in many publications; however, reproduction of hemodynamic changes due to physical activities, such as rest versus moderate exercise, need to be investigated. We developed a new design for patient specific coronary phantoms, which allow adjustable physiological variables such as coronary distal resistance and coronary compliance in patients with coronary artery disease. The new design was tested in precise benchtop experiments and compared with a theoretical Windkessel electrical circuit equivalent, that models coronary flow and pressure using arterial resistance and compliance.

Methods
Five phantoms from patients who underwent clinically indicated elective invasive coronary angiography were built from CCTA scans using multi-material 3D printing. Each phantom was used in a controlled flow system where patient specific flow conditions were simulated by a programmable cardiac pump. To simulate the arteriole and capillary beds flow resistance and the compliance for various physical activities, we designed a three-chamber outlet system which controls the outflow dynamics of each coronary tree. Benchtop pressure measurements were recorded using sensors embedded in each of the main coronary arteries. Using the Windkessel model, patient specific flow equivalent electrical circuit models were designed for each coronary tree branch, and flow in each artery was determined for known inflow conditions. Local flow resistances were calculated through Poiseuille’s Law derived from the radii and lengths of the coronary arteries using CT angiography based multi-planar reconstructions. The coronary stenosis flow rates from the benchtop and the electrical models were compared to the localized flow rates calculated from invasive pressure measurements recorded in the angio-suites.

Results
The average Pearson correlations of the localized flow rates at the location of the stenosis between each of the models (Benchtop/Electrical, Benchtop/Angio, Electrical/Angio) are 0.970, 0.981, and 0.958 respectively.

Conclusions
3D printed coronary phantoms can be used to replicate the human arterial anatomy as well as blood flow conditions. It displays high levels of correlation when compared to hemodynamics calculated in electrically-equivalent coronary Windkessel models as well as invasive angio-suite pressure measurements.
Beyond the Sexualized Colonial Narrative: Undoing the Visual History of Kisaeng in Colonial Korea
Authors: Rhee Jooyeon 
Published: April 2022

Abstract
This article examines visual representations of kisaeng (courtesans) in photographs and photo postcards, produced by Japanese entrepreneurs and kisaeng themselves, by viewing them as a contentious site of the historical memory of Japanese colonialism. It problematizes the nation-focused narratives on kisaeng in postcolonial South Korea as these narratives fail to recognize the complex dimension of the image-making process that cannot be fully grasped by the dialectic of the colonial aggressor and its victim. Instead, this article shows how kisaeng exercised their agency by actively engaging in producing visual images of themselves as a politically conscious response to the colonial reality. The author pays special attention to visual images appearing in the magazine Changhan, which was established by a group of kisaeng, to underscore women’s political intervention in the visual regime of colonial capitalism. The women’s voices embedded in Changhan are crucial, since they not only problematize their othered social position constructed by colonial capitalism and patriarchy but also lead us to investigate their interventions in the politics of representation that moved strategically across tradition and modernity while shifting their position from object to subject, and vice versa, revealing their tactical maneuver of the technological implications of visual politics.
A 3D molecular map of the cavefish neural plate illuminates eye-field organization and its borders in vertebrates
Authors: Agnès François , Torres-Paz Jorge , Michel Pauline , Rétaux Sylvie 
Published: April 2022

ABSTRACT
The vertebrate retinas originate from a specific anlage in the anterior neural plate called the eye field. Its identity is conferred by a set of ‘eye transcription factors’, whose combinatorial expression has been overlooked. Here, we use the dimorphic teleost Astyanax mexicanus, which develops proper eyes in the wild type and smaller colobomatous eyes in the blind cavefish embryos, to unravel the molecular anatomy of the eye field and its variations within a species. Using a series of markers (rx3, pax6a, cxcr4b, zic1, lhx2, emx3 and nkx2.1a), we draw a comparative 3D expression map at the end of gastrulation/onset of neurulation, which highlights hyper-regionalization of the eye field into sub-territories of distinct sizes, shapes, cell identities and combinatorial gene expression levels along the three body axes. All these features show significant variations in the cavefish natural mutant. We also discover sub-domains within the prospective telencephalon and characterize cell identities at the frontiers of the eye field. We propose putative fates for some of the characterized eye-field subdivisions, and suggest the existence of a trade-off between some subdivisions in the two Astyanax morphs on a micro-evolutionary scale.
Premeiotic endoreplication is essential for obligate parthenogenesis in geckos
Authors: Dedukh Dmitrij , Altmanová Marie , Klíma Jiří , Kratochvíl Lukáš 
Published: April 2022

ABSTRACT
Obligate parthenogenesis evolved in reptiles convergently several times, mainly through interspecific hybridization. The obligate parthenogenetic complexes typically include both diploid and triploid lineages. Offspring of parthenogenetic hybrids are genetic copies of their mother; however, the cellular mechanism enabling the production of unreduced cells is largely unknown. Here, we show that oocytes go through meiosis in three widespread, or even strongly invasive, obligate parthenogenetic complexes of geckos, namely in diploid and triploid Lepidodactylus lugubris, and triploid Hemiphyllodactylus typus and Heteronotia binoei. In all four lineages, the majority of oocytes enter the pachytene at the original ploidy level, but their chromosomes cannot pair properly and instead form univalents, bivalents and multivalents. Unreduced eggs with clonally inherited genomes are formed from germ cells that had undergone premeiotic endoreplication, in which appropriate segregation is ensured by the formation of bivalents made from copies of identical chromosomes. We conclude that the induction of premeiotic endoreplication in reptiles was independently co-opted at least four times as an essential component of parthenogenetic reproduction and that this mechanism enables the emergence of fertile polyploid lineages within parthenogenetic complexes.
Dopey proteins are essential but overlooked regulators of membrane trafficking
Authors: Molière Adrian , Beer Katharina B. , Wehman Ann M. 
Published: April 2022

ABSTRACT
Dopey family proteins play crucial roles in diverse processes from morphogenesis to neural function and are conserved from yeast to mammals. Understanding the mechanisms behind these critical functions could have major clinical significance, as dysregulation of Dopey proteins has been linked to the cognitive defects in Down syndrome, as well as neurological diseases. Dopey proteins form a complex with the non-essential GEF-like protein Mon2 and an essential lipid flippase from the P4-ATPase family. Different combinations of Dopey, Mon2 and flippases have been linked to regulating membrane remodeling, from endosomal recycling to extracellular vesicle formation, through their interactions with lipids and other membrane trafficking regulators, such as ARL1, SNX3 and the kinesin-1 light chain KLC2. Despite these important functions and their likely clinical significance, Dopey proteins remain understudied and their roles elusive. Here, we review the major scientific discoveries relating to Dopey proteins and detail key open questions regarding their function to draw attention to these fascinating enigmas.
The capture of crude oil droplets by filter feeders at high and low Reynolds numbers
Authors: Letendre Francis , Cameron Christopher B. 
Published: April 2022

ABSTRACT
Crustacean filter feeders capture oil droplets with the use of their ramified appendages. These appendages behave as paddles or sieves, based on the system's Reynolds number. Here, we used high-speed videography, scanning electron microscopy and fluid mechanics to study the capturing mechanisms of crude oil droplets and the filtering appendage's wettability by two species of barnacles (Balanus glandula and Balanus crenatus) and of the freshwater cladoceran Daphnia magna. Our results show that barnacle appendages behave as paddles and capture droplets in their boundary layers at low Reynolds number. At high Reynolds number, droplets are most likely to be captured via direct interception. There is an intermediate range of Reynolds number where droplets can be captured by both mechanisms at the same time. Daphnia magna captures droplets in the boundary layers of the third and fourth pair of thoracic legs with a metachronal motion of the appendages. All studied surfaces were revealed to be highly lipophobic, demonstrating captured oil droplets with high contact angles. We also discuss implications of such capture mechanisms and wettability on potential ingestion of crude oil by filter feeders. These results further our understanding of the capture of crude oil by filter feeders, shedding light on the main entry point of oil in marine food webs.
Relative Skeletal Maturity and Performance Test Outcomes in Elite Youth Middle Eastern Soccer Players
Authors: LOLLI LORENZO , JOHNSON AMANDA , MONACO MAURICIO , DI SALVO VALTER , GREGSON WARREN 
Published: April 2022

ABSTRACT

Purpose
This study aimed to explore the influence of differences in relative skeletal maturity on performance test outcomes in elite youth soccer players from the Middle East.

Methods
We integrated skeletal age and performance assessments using mixed-longitudinal data available for 199 outfield players (chronological age range, 11.7 to 17.8 yr) enrolled as academy student-athletes (annual screening range, 1 to 5 visits). Skeletal age was determined as per the Tanner–Whitehouse II protocol. Relative maturity was calculated as the difference (∆) between Tanner–Whitehouse II skeletal age minus chronological age. Performance test outcomes of interest were 10-m sprinting, 40-m sprinting, countermovement jump height, and maximal aerobic speed. Separate random-effects generalized additive models quantified differences in performance test outcomes by relative skeletal maturity. Estimated differences were deemed practically relevant based on the location of the confidence interval (95% CI) against minimal detectable change values for each performance test outcome.

Results
For 40-m sprinting, differences of +0.51 s (95% CI, +0.35 to +0.67 s) and +0.62 s (95% CI, +0.45 to +0.78 s) were practically relevant for relative maturity status of ∆ = −1.5 yr versus ∆ = +0.5 and ∆ = +1 yr, respectively. For countermovement jump height, a difference of −8 cm (95% CI, −10 to −5 cm) was practically relevant for ∆ = −1.5 yr versus ∆ = +1 yr relative maturity status comparison. Effects for 10-m sprinting and maximal aerobic speed were unclear.

Conclusions
Integration of skeletal age and performance assessments indicated that conventional maturity status classification criteria were inconsistent to inform player development processes in our sample. Between-player differences in test performance may depend on a substantial delay in skeletal maturation (∆ ≤ −1.5 yr) and the performance outcome measure.
Mitochondrial Mass of Naïve T Cells Is Associated with Aerobic Fitness and Energy Expenditure of Active and Inactive Adults
Authors: ALLEY JESSICA R. , VALENTINE RUDY J. , KOHUT MARIAN L. 
Published: April 2022

ABSTRACT

Purpose
Chronic exercise training is known to induce metabolic changes, but whether these adaptations extend to lymphocytes and how this may affect immune function remains largely unknown. This study was conducted to determine the extent to which mitochondrial characteristics of naïve T cells differ according to fitness status and to further examine the energy production pathways of cells from aerobically trained and inactive participants.

Methods
Blood was collected from 30 aerobically active (>6 h·wk−1) or inactive (<90 min·wk−1) men and women. Naïve T cell mitochondrial mass, membrane potential, and biogenesis were assessed with flow cytometry. Participants completed a treadmill maximal oxygen consumption (V̇O2peak) test and wore a physical activity monitor for 1 wk. In a subset of participants, naïve CD8+ T cell activation-induced glycolytic and mitochondrial ATP production was measured.

Results
Active participants exhibited 16.7% more naïve CD8+ T cell mitochondrial mass (P = 0.046), 34% greater daily energy expenditure (P < 0.001), and 39.6% higher relative V̇O2peak (P < 0.001), along with 33.9% lower relative body fatness (P < 0.001). Among all participants, naïve CD8+ T cell mitochondrial mass was correlated with estimated energy expenditure (r = 0.36, P = 0.048) and V̇O2peak (r = 0.47, P = 0.009). There were no significant differences in ATP production, mitochondrial biogenesis, or mitochondrial membrane potential between active and inactive groups.

Conclusions
This is the first study to examine the effects of aerobic exercise training status on metabolic parameters within human naïve T cells. Findings suggest that mitochondrial adaptations in certain immune cell types are positively associated with aerobic fitness and energy expenditure. This study provides a foundation for future development of prophylactic and therapeutic interventions targeting specific immune cell subsets to improve the immune response and overall health.
Effects of Exercise Training on PPARβ/δ Expression in Skeletal Muscle of Rats with Spontaneous Hypertension
Authors: YANG MINGXUAN , PAN YANXIA , LI KUNHUI , CHEN XIUYUN , LI MINYAN , LIN JIANPING , LI MING , LIN CHENG 
Published: April 2022

ABSTRACT

Purpose
This study aimed to identify the relationship and mechanism between skeletal muscle peroxisome proliferator–activated receptor β/δ (PPARβ/δ) and spontaneous hypertension.

Methods
Rats were divided into four groups (n = 10): spontaneous hypertensive rats exercise group (SHR-E), spontaneous hypertensive rats sedentary group (SHR-S), Wistar-Kyoto control rats exercise group (WKY-E), and Wistar-Kyoto control rats sedentary group (WKY-S). Although the sedentary groups were placed on the treadmill without moving during the training sessions, the exercise groups were forced to run on a treadmill for 8 wk, 1 h·d−1, 5 d·wk−1. After training, the density and area of gastrocnemius microvessels were observed. PPARβ/δ, vascular endothelial growth factor A (VEGFA), superoxide dismutase 2 (SOD-2), and nitric oxide synthase in gastrocnemius were measured by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot.

Results
Except the sixth week of age, the systolic blood pressure of SHR-S was significantly higher than that of WKY-S at all time periods. Exercise significantly reduced systolic blood pressure in SHR rats. Compared with the SHR-S group, the WKY-S group had significantly higher PPARβ/δ protein level and density of skeletal muscle microvessels. Eight weeks of exercise increased the PPARβ/δ, SOD-2, VEGFA, and microvessel density and area in the skeletal muscle of SHR.

Conclusions
Exercise training promoted PPARβ/δ mRNA and protein-level expression of PPARβ/δ, SOD-2 and VEGFA in skeletal muscle, thus increasing the density and area of skeletal muscle blood vessels. These regulations contribute to the reduction of peripheral vascular resistance. This may be a potential mechanism of exercise to reduce blood pressure.
An Evidence-Based Narrative Review of Mechanisms of Resistance Exercise–Induced Human Skeletal Muscle Hypertrophy
Authors: LIM CHANGHYUN , NUNES EVERSON A. , CURRIER BRAD S. , MCLEOD JONATHAN C. , THOMAS AARON C. Q. , PHILLIPS STUART M. 
Published: April 2022

ABSTRACT

Skeletal muscle plays a critical role in physical function and metabolic health. Muscle is a highly adaptable tissue that responds to resistance exercise (RE; loading) by hypertrophying, or during muscle disuse, RE mitigates muscle loss. Resistance exercise training (RET)–induced skeletal muscle hypertrophy is a product of external (e.g., RE programming, diet, some supplements) and internal variables (e.g., mechanotransduction, ribosomes, gene expression, satellite cells activity). RE is undeniably the most potent nonpharmacological external variable to stimulate the activation/suppression of internal variables linked to muscular hypertrophy or countering disuse-induced muscle loss. Here, we posit that despite considerable research on the impact of external variables on RET and hypertrophy, internal variables (i.e., inherent skeletal muscle biology) are dominant in regulating the extent of hypertrophy in response to external stimuli. Thus, identifying the key internal skeletal muscle–derived variables that mediate the translation of external RE variables will be pivotal to determining the most effective strategies for skeletal muscle hypertrophy in healthy persons. Such work will aid in enhancing function in clinical populations, slowing functional decline, and promoting physical mobility. We provide up-to-date, evidence-based perspectives of the mechanisms regulating RET-induced skeletal muscle hypertrophy.