bluuebunny/crossref_metadata_2025_split huggingface dataset:
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium
Authors: Huang Huimin , Gong Zizhen , Zhu Xuejun , Tan Wen‐song , Cai Haibo 
Published: June 2022

AbstractAs a water‐soluble macromolecule polysaccharide, xanthan gum (XG) has several biological activities, such as antitumor, antiviral, and immunomodulatory function. However, the effect of XG on the proliferation and cytotoxicity of cytokines induced killer (CIK) cells is rarely studied. In this study, the effect of XG on CIK cells derived from peripheral blood was investigated by analyzing the expansion fold of total cells, phenotype, cytotoxicity, degranulation, and apoptosis in serum‐free medium. The results showed that the expansion fold of total cells with 100 μg/ml XG which molecule weight is 2.95 × 106 Da reached 4534.0 folds, significantly higher than that without XG (1299.0 folds, p < 0.05). The percentage of main effector cells‐CD3+CD56+ cells increased to 25.5% and the cytotoxic activity of CIK cells increased to 45.3%. The cell proportions of expression granzyme B and perforin that related to cytotoxicity in CIK cells reached 53.6% and 48.3%, respectively, significantly higher than 27.5% and 37.5% in the group without XG (p < 0.05). Collectively, XG could stimulate the ex vivo expansion of CIK cells and enhance the cytotoxicity of expanded CIK cells. The above results provide technical support for optimizing the expansion process of CIK cells ex vivo.
Hyaluronic acid filler injection for deep nasolabial folds: A novel intraoral approach
Authors: Yi Kyu‐Ho , Lee Jong‐Jin , Hur Hye‐Won , Bae Hyungkyu , Kim Hee‐Jin 
Published: June 2022

AbstractHyaluronic acid filler injection is a minimally invasive procedure for facial rejuvenation that involves injecting filling materials to correct the volume or augment specific areas in the face. Deep nasolabial folds are a common concern in aging people. The simplest way to correct a deep nasolabial fold to rejuvenate the face is to inject Ristow's space with hyaluronic acid fillers. However, conventional injection methods, such as percutaneous injections using a needle, can cause severe complications, such as skin necrosis or blindness due to intravascular injections. Therefore, the aim of the present study was to introduce a safe technique for intraoral filler injections in deep nasolabial folds and review related anatomic features to evaluate the safety of this technique.
Candidate therapeutic agents in a newly established triple wild‐type mucosal melanoma cell line
Authors: Shi Chaoji , Gu Ziyue , Xu Shengming , Ju Houyu , Wu Yunteng , Han Yong , Li Jiayi , Li Chuwen , Wu Jing , Wang Lizhen , Li Jiang , Zhou Guoyu , Ye Weimin , Ren Guoxin , Zhang Zhiyuan , Zhou Rong 
Published: June 2022

AbstractBackgroundMucosal melanoma has characteristically distinct genetic features and typically poor prognosis. The lack of representative mucosal melanoma models, especially cell lines, has hindered translational research on this melanoma subtype. In this study, we aimed to establish and provide the biological properties, genomic features and the pharmacological profiles of a mucosal melanoma cell line that would contribute to the understanding and treatment optimization of molecularly‐defined mucosal melanoma subtype.MethodsThe sample was collected from a 67‐year‐old mucosal melanoma patient and processed into pieces for the establishment of cell line and patient‐derived xenograft (PDX) model. The proliferation and tumorigenic property of cancer cells from different passages were evaluated, and whole‐genome sequencing (WGS) was performed on the original tumor, PDX, established cell line, and the matched blood to confirm the establishment and define the genomic features of this cell line. AmpliconArchitect was conducted to depict the architecture of amplified regions detected by WGS. High‐throughput drug screening (HTDS) assay including a total of 103 therapeutic agents was implemented on the established cell line, and selected candidate agents were validated in the corresponding PDX model.ResultsA mucosal melanoma cell line, MM9H‐1, was established which exhibited robust proliferation and tumorigenicity after more than 100 serial passages. Genomic analysis of MM9H‐1, corresponding PDX, and the original tumor showed genetic fidelity across genomes, and MM9H‐1 was defined as a triple wild‐type (TWT) melanoma subtype lacking well‐characterized “driver mutations”. Instead, the amplification of several oncogenes, telomerase reverse transcriptase (TERT), v‐Raf murine sarcoma viral oncogene homolog B1 (BRAF), melanocyte Inducing transcription factor (MITF) and INO80 complex ATPase subunit (INO80), via large‐scale genomic rearrangement potentially contributed to oncogenesis of MM9H‐1. Moreover, HTDS identified proteasome inhibitors, especially bortezomib, as promising therapeutic candidates for MM9H‐1, which was verified in the corresponding PDX model in vivo.ConclusionsWe established and characterized a new mucosal melanoma cell line, MM9H‐1, and defined this cell line as a TWT melanoma subtype lacking well‐characterized “driver mutations”. The MM9H‐1 cell line could be adopted as a unique model for the preclinical investigation of mucosal melanoma.
Comprehensive global collaboration in the care of 1182 pediatric oncology patients over 12 years: The Iraqi–Italian experience
Authors: Al‐Jadiry Mazin Faisal , Uccini Stefania , Testi Anna Maria , Moleti Maria Luisa , Alsaadawi Adil Rabeea , Al‐Darraji Amir Fadhil , Al‐Saeed Raghad Majid , Faraj Al‐Badri Safaa A. , Sabhan Ahmed Hatem , Ghali Hasanein Habeeb , Fadhil Samaher Abdulrazzaq , Abed Wisam Majeed , Ameen Najiha Ahmed , Abed Yasir Saadoon , Yousif Fawaz Salim , Abed Aseel Rashid , Hussein Hanadi Munaf , Shkara Ahmed Mudhafar , Piciocchi Alfonso , Mohamed Sara , Ruco Luigi , Qaddoumi Ibrahim , Al‐Hadad Salma Abbas 
Published: June 2022

AbstractBackgroundIraq's health care system has gradually declined after several decades of wars, terrorism, and UN economic sanctions. The Oncology Unit at Children's Welfare Teaching Hospital (CWTH) in Baghdad was lacking basic facilities and support. To address this shortcoming, a humanitarian and educational partnership was established between CWTH and Sapienza University of Rome (SUR).MethodsWe investigated the outcomes of 80 online and 16 onsite educational sessions and 142 teleconsultation sessions from 2006 to 2014. We also determined the outcomes of pathology reviews by SUR of 1216 tissue specimens submitted by CWTH from 2007 until 2019 for second opinions. The primary outcomes were discordance, concordance, and changes among clinical diagnoses and pathology review findings. The measures included the frequency of teleconsultation and tele‐education sessions, the topics discussed in these sessions, and the number of pathology samples requiring second opinions.FindingsA total of 500 cases were discussed via teleconsultations during the study period. The median patient age was 7 years (range, 24 days to 16·4 years), and the cases comprised 79 benign tumors, 299 leukemias, 120 lymphomas, and 97 solid tumors. The teleconsultation sessions yielded 27 diagnostic changes, 123 confirmed diagnoses, and 13 equivocal impacts. The pathology reviews by SUR were concordant for 996 (81·9%) cases, discordant for 186 (15·3%), and inconclusive for 34 (2·8%). The major cause of discordance was inadequate immunohistochemical staining. The percentage of discordance markedly decreased over time (from 40% to 10%). The cause of the improvement is multifactorial: training of two CWTH pathologists at SUR, better immunohistochemical staining, and the ongoing clinical and pathologic telemedicine activities. The partnership yielded 12 publications, six posters, and three oral presentations by CWTH investigators.InterpretationThe exchange of knowledge and expertise across continental boundaries meaningfully improved the diagnoses and management of pediatric cancer at CWTH.
PDGF‐BB‐enriched collagen matrix to treat multiple gingival recessions with the tunneled coronally advanced flap
Authors: Barootchi Shayan , Giannobile William V. , Tavelli Lorenzo 
Published: June 2022

AbstractBackgroundWith technological advancements in reconstructive periodontology, traditional protocols for the treatment of gingival recessions (GRs) can be challenged. This manuscript presents preliminary findings of a novel minimally‐invasive approach for the regenerative treatment of multiple adjacent GR defects.MethodsTwo healthy adults were treated as part of this study. Multiple adjacent GRs in both subjects (1 in the mandible, and 1 in the maxilla) were treated employing a tunneled coronally advanced flap (TCAF) design, with the application of a cross‐linked collagen matrix (CCM) that was enriched with recombinant human platelet‐derived growth factor‐BB (PDGF‐BB) that was also applied on the prepared root surfaces. Clinical, ultrasonographic, esthetic, and patient‐reported outcomes were observed at approximately 6‐ and 18‐month time points.ResultsAll sites healed uneventfully after the treatments. Complete root coverage was achieved and maintained throughout the follow‐up observations, from 6 to 18 months. Patients reported minimal discomfort and reduction of dentinal hypersensitivity at the augmented sites. The areas augmented with CCM + PDGF‐BB revealed an increased soft tissue thickness relative to baseline (pretreatment) measures, as well as reduction in the level of the facial bone dehiscences.ConclusionThis article describes the success of two cases of a novel minimally invasive regenerative approach for the treatment of multiple adjacent GR defects by the TCAF, using a CCM loaded with PDGF‐BB. This approach offers potential as a minimally‐invasive method to repair multiple adjacent GRs.
A spatial augmented reality based circuit experiment and comparative study with the conventional one
Authors: Pan Zhigeng , Wang Zihan , Yuan Qingshu , Meng Qianyu , Liu Jiaxin , Shou Kailiang , Sun Xiaoyan 
Published: June 2022

AbstractHow to intuitively illustrate phenomena while retaining good operation experiences is a key issue in experimental learning. Virtual experiments with desktop environments, handheld devices, or headsets can show invisible phenomena for students. However, they are either visuo‐tactile inconsistent in space or with heavy physical burdens, causing bad experiences. A spatial augmented reality (SAR) based circuit experiment is developed. It allows students to interact with 3D (three dimensional) printed tangible objects without wearing any devices, having low physical burdens. Objects' poses are tracked using Microsoft Azure Kinect and inertial measurement unit. Virtual phenomena are projected onto the tangible objects and tabletop accordingly. Physical input and virtual output space are completely fused from students' view. It also offers efficient operation manners to students. A questionnaire comparing user experiences between the SAR and conventional experiment reveals that the former has a better learning experience.
Parallel‐branch network for 3D human pose and shape estimation in video
Authors: Wu Yuanhao , Wang Chenxing 
Published: June 2022

AbstractHuman pose and shape estimation have developed rapidly, where a skinned multi‐person linear (SMPL) approach performs excellent recently. However, the prior template of the human body in the SMPL model is fixed, thus a deviation may be resulted in the reconstructed body shape if a human body acts sharp movements such as sporting or dancing. To address this problem, we propose a parallel‐branch network including a designed spatial–temporal (ST) branch and a SMPL branch. The ST branch essentially performs the 2D‐to‐3D lifting for more accurate joint prediction, by the designed spatial transformer and temporal transformer. The 3D joints from the ST branch are used to supervise the 3D joints from the SMPL branch and further correct the deviation of the SMPL model. Experiments on some popular benchmarks like 3DPW and MPI‐INF‐3DHP show that our method has better performance than other methods with video input. Our code is available athttps://automation.seu.edu.cn/wcx/list.htm
Novel Hydrazono‐2‐Iminothiazolidin‐4‐Ones Based on a Monoterpenic Skeleton as Potential Antitumor Agents: Synthesis, DFT Studies, in Vitro Cytotoxicity, Apoptosis Inducing Properties and Molecular Docking
Authors: Fawzi Mourad , Oubella Ali , El Mansouri Az‐Eddine , Bimoussa Abdoullah , Ketatni El Mostafa , Saadi Mohamed , El Ammari Lahcen , Ait Itto Moulay Youssef , Morjani Hamid , Auhmani Aziz 
Published: June 2022

AbstractA series of novel 2‐iminothiazolidin‐4‐one analogs have been synthesized from limonaketone, and structurally characterized by HR‐MS, 1H‐NMR and 13C‐NMR spectroscopy techniques, and the structure of compound 4 was elucidated by XRD. The newly synthesized products were biologically evaluated in vitro for their cytotoxic activity against human cancer cell lines HT‐1080, A549, and MCF‐7. Thiazolidinones 9 and 10 were the most active compounds in HT‐1080 cell lines (IC50=15.85±1.75 and 16.13±1.55 μM, respectively). The apoptosis induction of the derivatives 9 and 10 were studied using annexin V staining, caspase‐3/7 activity and cell cycle analysis. Compound 10 showed the highest ability of apoptosis induction and caspase‐3/7 activation associated with S‐phase growth arrest in HT‐1080. Meanwhile, compound 9 has a moderate apoptotic effect and G0/G1‐phase arrest in the after‐mentioned cell. The molecular docking suggested that compounds 9 and 10 formed stable ligand‐caspase‐3 complexes. Besides, the presence of phenyl moiety in ligand 10 is responsible for the enhancement of the caspase‐3 activation by the apparition of two additional hydrogen bonds with Cys163 and Gln161amino acids.
The Role of Vacancies in a Ti2CTx MXene‐Derived Catalyst for Butane Oxidative Dehydrogenation
Authors: Ronda‐Lloret M. , Slot T. K. , van Leest N. P. , de Bruin B. , Sloof W. G. , Batyrev E. , Sepúlveda‐Escribano A. , Ramos‐Fernandez E. V. , Rothenberg G. , Shiju N. R. 
Published: June 2022

AbstractMXenes are a new family of 2D carbides or nitrides that have attracted attention due to their layered structure, tunable surface groups and high electrical conductivity. Here, we report for the first time that the Ti2CTx MXene catalyses the selective oxidative dehydrogenation of n‐butane to butenes and 1,3‐butadiene. This catalyst showed higher intrinsic activity compared to a commercial TiC and TiO2 samples in terms of C4 olefin formation rate. We propose that the stabilisation of structural vacancies and the change in composition (from a carbide to a mixed phase oxide) in the MXene causes its higher catalytic activity. These vacancies can lead to a higher concentration of unpaired electrons in the MXene‐derived material, enhancing its nucleophilic properties and favouring the production of olefins.
Emerging leaders: Leadership development experiences of counselor education doctoral students
Authors: DeDiego Amanda C. , Chan Christian D. , Basma Dareen 
Published: June 2022

AbstractCounselor educators often serve in leadership roles in professional organizations. These leadership activities often begin as graduate students through leadership development programs (e.g., Emerging Leaders) within professional counseling organizations. This basic interpretive qualitative study explored leadership development experiences of 24 counselor education doctoral students yielding themes informing leadership development.
Machine Learning Prediction of Structure‐Performance Relationship in Organic Synthesis
Authors: Yang Li‐Cheng , Zhu Lu‐Jing , Zhang Shuo‐Qing , Hong Xin 
Published: June 2022

Comprehensive SummaryData‐driven approach has emerged as a powerful strategy in the construction of structure‐performance relationships in organic synthesis. To close the gap between mechanistic understanding and synthetic prediction, we have made efforts to implement mechanistic knowledge in machine learning modelling of organic transformation, as a way to achieve accurate predictions of reactivity, regio‐ and stereoselectivity. We have constructed a comprehensive and balanced computational database for target radical transformations (arene C—H functionalization and HAT reaction), which laid the foundation for the reactivity and selectivity prediction. Furthermore, we found that the combination of computational statistics and physical organic descriptors offers a practical solution to build machine learning structure‐performance models for reactivity and regioselectivity. To allow machine learning modelling of stereoselectivity, a structured database of asymmetric hydrogenation of olefins was built, and we designed a chemical heuristics‐based hierarchical learning approach to effectively use the big data in the early stage of catalysis screening. Our studies reflect a tiny portion of the exciting developments of machine learning in organic chemistry. The synergy between mechanistic knowledge and machine learning will continue to generate a strong momentum to push the limit of reaction performance prediction in organic chemistry.
How do you get into this specific field? Could you please share some experiences with our readers?Based on my study experience in Prof. Houk's lab and Prof. Nørskov's lab, my major idea since the beginning of my lab is to combine the key design principles of homogeneous catalysis (transition state model) and heterogeneous (scaling relationship) catalysis. This idea eventually evolved to our explorations of mechanism‐based machine learning in organic chemistry.How do you supervise your students?I try my best to give them enough space and freedom, so they can experience the joy in chemistry research.What are your hobbies?I enjoy science fiction movies and novels.What is the most important personality for scientific research?Chemistry has unlimited frontiers. Targeting a hardcore question, developing someone's own approach is the most important merit in fundamental scientific research.How do you keep balance between research and family?Work‐life balance is certainly one of the biggest challenges for junior faculty. I try to work in fragmented time, so I would be available for both my family and my students.Who influences you mostly in your life?My high‐school experience in Chemistry Olympiad has influenced me dramatically, which cultivated my independent learning ability to tackle new questions. This has helped me a lot throughout my career.
Metal‐Free Visible‐Light‐Induced Atom‐Transfer Radical Addition Reaction of Alkenes/Alkynes with ICH2CN
Authors: Pan Chunxiang , Meng Yunyan , Deng Yao , Zhou Bingjie , Chen Jingchao , He Zhenxiu , Sun Weiqing , Khan Ruhima , Fan Baomin 
Published: June 2022

Comprehensive SummaryNew blue‐light promoted methods for the addition of iodoacetonitrile (ICH2CN) to alkene/alkynes have been developed. The reaction led to the formation of cyanoalkylated products when conjugated alkene/styrene was used. In the case of alkynes and non‐conjugated alkenes, iodoalkylation takes place furnishing the corresponding alkenyl iodides and alkyl iodides, respectively. Notably, trans‐alkenyl iodides were obtained as the major product. A plausible reaction mechanism is also proposed.
Self‐assembly Behavior of Metal Halide Perovskite Nanocrystals
Authors: Yang Zhuoying , Peng Shaomin , Lin Fan , Wang Pengfei , Xing Guichuan , Yu Lin 
Published: June 2022

Comprehensive SummaryThe self‐assembled metal halide perovskite (MHP) nanocrystal superlattices have attracted many researchers due to their exceptional optical and electrical properties. The bottom‐up self‐assembly can be facile to generate ideal and periodic structures. The superlattices’ stability can be improved and the photoluminescence lifetime can be extended by an order of magnitude. However, due to lack of a comprehensive and systematic understanding of the internal interactions on self‐assembled processes now, superlattices cannot be obtained controllably and play full use of their advantages. Therefore, gaining a deep insight of interaction forces about self‐assemblies is the premise of designing and controlling the degree of self‐assembly so as to prepare ideal materials. In this review, the definitions and functions of driving forces including van der Waals forces, electrostatic interactions and hydrogen bonds are discussed. Subsequently, we aim to explore the dominant factors affecting the driving forces, which can make a difference in the process of MHP self‐assembly. Based on current researches, we emphasize on three aspects−the core of nanocrystals, surface ligands and solvents−to clarify their critical roles in controlling the driving forces. Finally, the outlooks and perspectives of how to facilitate the MHP self‐assembly and their application on blue light emitting diodes are discussed.
Modeling social network behavior spread based on group cohesion under uncertain environment
Authors: Li Weimin , Deng Zhibin , Zhou Xiaokang , Jin Qun , Sheng Bin 
Published: June 2022

SummaryBehavior is autonomous, convergent, and uncertain, which brings challenges to the modeling of social network behavior spread. In this article, we propose a behavior spread model based on group cohesion under uncertain environments. First, for behavioral convergence, we define group cohesion to quantify the convergent effects of group. Second, based on the game theory to model the autonomy of behavior, according to the characteristics of the game payoffs changing with time and the depth of spread, and integrating group cohesion, a dynamic game payoffs calculation method is designed. Finally, aiming at the uncertainty of behavior, a group behavior spread model based on random utility theory is established. Experiments on multiple real social network behavior spread datasets demonstrate the effectiveness of the proposed model in modeling and predicting behavior spread processes under uncertain environments.
Incorporating Near‐Pseudocapacitance Insertion Ni/Co‐Based Hexacyanoferrate and Low‐Cost Metallic Zn for Aqueous K‐Ion Batteries
Authors: Luo Ping , Huang Zhen , Zhang Wenwei , Liu Chang , Liu Gangyuan , Huang Meng , Xiao Yao , Luo Hongyu , Qu Zhuo , Dong Shijie , Xia Lu , Tang Han , An Qinyou 
Published: June 2022

AbstractThe limited availability of cathode materials with high specific capacity and significant cycling stability for aqueous K‐ion batteries (AKIBs) hinder their further development owing to the large radius of K+ (1.38 Å). Prussian blue and its analogs with a three‐dimensional frame structure possessing special energy storage mechanism are promising candidates as cathode materials for AKIBs. In this study, K0.2Ni0.68Co0.77Fe(CN)6 ⋅ 1.8H2O (KNCHCF) was prepared as a cathode material for AKIBs. Both the electrochemical activity of Co ions and the near‐pseudocapacitance intercalation of KNCHCF enhance K+ storage. Therefore, KNCHCF exhibits a superior capacity maintenance rate of 86 % after 1000 cycles at a high current density of 3.0 A g−1. The storage mechanism of K+ in AKIBs was revealed through ex situ X‐ray diffraction, ex situ Fourier transform infrared spectroscopy, and ex situ X‐ray photoelectron spectroscopy measurements. Moreover, the assembled K−Zn hybrid battery showed good cycling stability with 93.1 % capacity maintenance at 0.1 A g−1 after 50 cycles and a high energy density of 96.81 W h kg−1. Hence, KNCHCF may be a potential material for the development of AKIBs.