bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low disease activity of microscopic polyangiitis in patients with anti-myosin light chain 6 antibody that disrupts actin rearrangement necessary for neutrophil extracellular trap formation
Authors: Yoshinari Miku , Nishibata Yuka , Masuda Sakiko , Nakazawa Daigo , Tomaru Utano , Arimura Yoshihiro , Amano Koichi , Yuzawa Yukio , Sada Ken-Ei , Atsumi Tatsuya , Dobashi Hiroaki , Hasegawa Hitoshi , Harigai Masayoshi , Takasaki Yoshinari , Saito Masaya , Matsuo Seiichi , Makino Hirofumi , Ishizu Akihiro 
Published: September 2022

Abstract
Background
Neutrophil extracellular traps (NETs) are critically involved in microscopic polyangiitis (MPA) pathogenesis, and some patients with MPA possess anti-NET antibody (ANETA). Anti-myosin light chain 6 (MYL6) antibody is an ANETA that affects NETs. This study aimed to determine the significance of anti-MYL6 antibody in MPA.
Methods
The influence of anti-MYL6 antibody on NET formation and actin rearrangement necessary for NET formation was assessed by fluorescent staining. An enzyme-linked immunosorbent assay was established to detect serum anti-MYL6 antibody, and the prevalence of this antibody in MPA was determined. Furthermore, the disease activity and response to remission-induction therapy of MPA were compared between anti-MYL6 antibody-positive and -negative MPA patients.
Results
Anti-MYL6 antibody disrupted G-actin polymerization into F-actin, suppressing phorbol 12-myristate 13-acetate-induced NET formation. Serum anti-MYL6 antibody was detected in 7 of 59 patients with MPA. The Birmingham vasculitis activity score (BVAS) of anti-MYL6 antibody-positive MPA patients was significantly lower than anti-MYL6 antibody-negative MPA patients. Among the nine BVAS evaluation items, the cutaneous, cardiovascular, and nervous system scores of anti-MYL6 antibody-positive MPA patients were significantly lower than anti-MYL6 antibody-negative MPA patients, although other items, including renal and chest scores, were equivalent between the two groups. The proportion of patients with remission 6 months after initiation of remission-induction therapy in anti-MYL6 antibody-positive MPA patients was significantly higher than in anti-MYL6 antibody-negative MPA patients.
Conclusions
Collective findings suggested that anti-MYL6 antibody disrupted actin rearrangement necessary for NET formation and could reduce the disease activity of MPA.
EnTube: A Dataset for YouTube Video Engagement Analytics
Authors: Le Truong , Nguyen-Thi Minh-Vuong , Le Huy , Vo Quoc-Thang , Le Tung , Nguyen Huy Tien 
Published: September 2022

Abstract

YouTube, one of the largest online video-sharing platforms today, has provided a place for content creators to share information and earn extra income. Anticipating whether a video will be engaged by viewers or not is an essential factor in helping video creators improve video content and quality before publishing. To facilitate this task, we build an annotated dataset of 23,738 videos collected from 72 YouTube channels in Vietnam that were in four categories (i.e., comedy, travel-and-events, education, science-and-technology) and published over 12 years. We evaluate a number of metrics for measuring video engagement to propose a novel measure which determines the engagement of a video via Q score. Using our proposed measure, we annotate videos with three levels of engagement including Engage, Neutral, and not Engage. From the supervised dataset, we constructed a multimodal model to infer the degree of engagement based on the content of a YouTube video such as title, audio, thumbnails, video, and tags. We believe our dataset and metric to be useful for engagement analytics as well as studies on social media content.
Early fluid-free macula and stability assessment: real world experience with Brolucizumab
Authors: Vadalà Maria , Grassi Maria Oliva , Alaimo Francesco , Moavero Chiara , Viggiano Pasquale , Boscia Francesco , Cillino Salvatore , Bonfiglio Vincenza Maria Elena 
Published: September 2022

Abstract
Purpose: Brolucizumab is the most recent anti-vascular endothelial growth factor (anti-VEGF) agent commercialized, approved for neovascular age-related macular degeneration (nAMD) as the first indication. A fluid-free retina and no recurrence of fluids are the new targets of retinal therapy, and early clinical experience with Brolucizumab shows strong effectiveness in drying retinal fluid. This study presents the results of real-world experience in treating nAMD naïve patients with intravitreal Brolucizumab.
Methods: This prospective observational study included 28 treatment-naïve eyes from two centres who underwent therapy with intravitreal injection of Brolucizumab for age-related neovascular macular degeneration. Several parameters were evaluated, including central retinal thickness (CRT), central retinal volume (CRV), intraretinal fluid (IRF), subretinal fluid (SRF), detachment of pigment retinal epithelium (PED), and subretinal hyper-reflective material (HRSM), from baseline up to 16 weeks.
Results: CTR and CRV reduced in a significant manner at w16 (p<0.0001 and p=0.01 respectively). SRF, IRF, PED, and HRSM almost disappeared in most cases. At w16, resolution of clinical activity was assessed at 86% to 89%, with PED showing a slower response. Interestingly, the cohort showed resolution of signs at w4 up to 82% of the eyes, after only one IVT-B. Visual acuity improved from 0.76 at baseline to 0.55 logMAR at w16 (p=0.002).
Conclusion: Our report strongly associated the effectiveness of the three-monthly induction treatment with a quick resolution of fluids in nAMD patients treated with Brolucizumab.
GADD34 activates p53 and may have utility as a marker of atherosclerosis
Authors: Tomiyoshi Go , Nakamura Rika , Shinmen Natsuko , Yoshida Yoichi , Mine Seiichiro , Machida Toshio , Kitamura Kenichiro , Iwase Katsuro , Iwadate Yasuo , Hiwasa Takaki , Kuroda Hideyuki 
Published: September 2022

Abstract
We previously identified growth arrest and DNA-damage-inducible gene 34 (GADD34) as a marker of ischemic stroke. In the present study, serum levels of anti-GADD34 antibodies were found to be significantly higher in patients with acute ischemic stroke or chronic kidney disease compared to healthy donors. We then examined the biological function of GADD34 by transfection into human osteosarcoma U2OS cells. Knockdown of GADD34 by siRNA resulted in enhanced cell proliferation, which was suppressed by knockdown of MDM2. Luciferase reporter assays revealed that the transactivation ability of p53 enhanced by genotoxic anticancer drugs such as camptothecin and etoposide was further potentiated by the presence of GADD34 and attenuated by co-transfection with p53 shRNA expression plasmids. Western blotting demonstrated increased p53 protein levels after treatment with camptothecin, which was also potentiated by GADD34 but suppressed by GADD34 siRNA, ATM siRNA, and wortmannin, an ATM inhibitor. GADD34 levels increased in response to treatment with camptothecin or adriamycin, and this increase was attenuated by MDM2 siRNA. Immunoprecipitation with anti-GADD34 antibodies followed by Western blotting with anti-MDM2 antibodies indicated ubiquitination of GADD34 is mediated by MDM2. Accordingly, GADD34 may function as a ubiquitination decoy to reduce p53 ubiquitination and increase p53 protein levels. Increased neuronal death due to activation of p53 by GADD34 may account for the elevated serum levels of anti-GADD34 antibodies observed in patients with acute ischemic stroke.
One script to solve it all – an open-source-based framework for a digital workflow based on WWTP data
Authors: Ahnert Markus , Hurzlmeier Stefan 
Published: September 2022

Abstract
The increasing use of digital technology has many advantages, but is insufficiently used in urban water management. This article describes a workflow based on detailed German rules and regulations for the use of routine data from wastewater treatment plants (WWTP) for design, assessment, benchmarking, and activated sludge modelling. Our workflows use data that are routinely collected. However, prior to use the data must first be checked. It includes data import, plausibility checks, data evaluation, and subsequent plant design. In addition, the data available in the system can be used to supply an activated sludge model of the plant as a basis for use in redesign and process optimisation. The main advantages of such an approach are time savings, increased quality, and a transparent and comprehensible procedure. Exemplary results are presented for individual elements of the workflow. We also provide a comprehensive classification of the question in the context of environmental data science. WWTP routine data bases are an important source for information needed for evaluation and optimisation.
Extinction Delay and Reinstated Fear Generalization
Authors: Callen Edward J. , Medders Collytte , Best Lauren 
Published: September 2022

Background:
After classical conditioning and extinction of fear, presentation of an unconditioned stimulus can produce a re-emergence of the extinguished fear response, a reinstatement effect. Previous research has reported that reinstatement of fear of contextual cues after a post-extinction delay could generalize over time to similar contexts.

Objective:
Two experiments were designed to investigate reinstated fear to a discrete stimulus with either a post-reinstatement delay (Experiment 1) or a post-extinction delay (Experiment 2).

Methods:
In Experiment 1 rat subjects were randomly assigned to four groups which received fear conditioning and extinction with a 3000 Hz tone CS. Reinstatement conditioning was then conducted with either the same or different CS, and testing for reinstated fear with the original CS was conducted after either a short or long delay following reinstatement. In Experiment 2 all procedures were the same except the long or short delay employed was between extinction and reinstatement instead of after reinstatement.

Results:
Reinstated fear increased with time to the same CS, and an increase in generalization of reinstated fear over time was observed with a different reinstatement CS (Experiment 1). When the delay employed was between extinction and reinstatement no increase in fear generalization was observed (Experiment 2).

Conclusion:
The results indicated support for the notion that reinstated fear to a discrete stimulus may generalize over time, but that generalization only takes place when reinstatement is administered a short interval after extinction. The results have implications for clinical behavior therapy treatments of anxiety disorders.
Role of Knowledge, Attitude and Practice Among the General Population of Saudi Arabia in Management of Second Wave of COVID-19
Authors: Al-Mohaithef Mohammed , Miraj Shaima Ali , Thomas Tintu , Zedan Haya , Saleem Shakir 
Published: September 2022

Introduction:
The highly infectious corona virus disease, described as a pandemic by the WHO, has been the focus of intense discussion world-wide due to its unpredictable nature and impact. The present study was undertaken to assess the health behavior of individuals as a result of their knowledge, attitudes and practice, which are important in containing the spread of such a pandemic in the community with anticipated future outcomes.

Methods:
This study was carried out to explore the influence of KAP of the Saudi population from Riyadh, Dammam, Jeddah, Medina, Abha, Qassim and other cities of KSA towards the second wave of the pandemic spike, and determine the acceptance of the population of changed behavioral measures.

Results:
About 90.2% of the respondents had strong knowledge and awareness about COVID-19 where as 80.7% showed positive attitudes, with a mean score of 29.52 ± 2.591 and 59.4% following good practices. About 75.5% of respondents regularly washed their hands, 73.7% used sanitizers effectively every time after touching some surface and 86.8% affirmed using masks regularly.

Conclusion:
The present data showed that irrespective of gender, age group, nationality, education level and occupation, majority of the population had adequate knowledge and positive attitude. However, it was found that good knowledge and positive attitude may not always culminate into good practice as evident from the study. This reflects that irrespective of the shortcomings, the Saudi government was successful in propagating right information across all sections of the society. The findings give valuable insight to policy makers and health officials to pinpoint gaps in these areas and bolster ongoing efforts to contain the pandemic and sustain the preventive measures implemented as part of the future strategic planning.
MEWUJUDKAN KESEJAHTERAAN KELUARGA MELALUI PERSPEKTIF FILOSOFIS HUKUM ISLAM
Authors: Mutaqim M. Akrom 
Published: September 2022

Proses untuk menuju kehidupan keluarga idaman merupakan suatu keinginan dan mimpi bagi setiap orang dalam menjalankan kehidupan berkeluarga. Selain itu, dalam mencapai sebuah keluarga idaman juga perlu dibutuhkan usaha lebih untuk mewujudkannya. Islam memiliki tuntunan dan hukum dimana dengan tercapainya keluarga yang sesuai dengan tujuan dari pernikahan yaitu sakinah adalah keluarga yang harmonis dan ideal, mawaddah, perasaan cinta terhadap pasangan, rahmah, cinta yang siap berkorban dan siap melindungi bagi siapa yang dicintai akan mewujudkan hasil dari keluarga idaman tersebut.
Pelaksanaan pembelajaran mengenai agama islam tentunya tak lepas dari pembelajaran hukum islam. Dalam hukum islam terdapat banyak sekali pembelajaran dan anjuran serta tuntunan untuk menjalani hidup baik dalam masyarakat maupun keluarga. Salah satu pembelajaran mengenai hukum islam yaitu hukum perkawinan, anjuran memilih pasangan, dan anjuran dalam menjalankan hidup dalam berumah tangga. Oleh karena itu, tidak dapat dipungkiri bahwa hukum islam sangatlah berperan bagi kelangsungan hidup bermasyarakat, yang akan mengatur atau mengarahkan guna mencapai tujuan dari sebuah keluarga yang harmonis.
Neurotoxoplasmose em portadores de síndrome da imunodeficiência humana: revisão de literatura
Authors: Ferreira Filho Victor Eddye , Mourão Maria Antonia Oakim , Hissi Paula Azevedo , Bittencourt Letícia Ayd , Caçador Alan Andrade , Soares Bernardo Mussi , Celes Vicente Carriello , Junger Henrique Daflon Fernandes , Fernandes Beatriz da Motta , Galhardo Maria Eulália Gouvea 
Published: September 2022

Objetivo: Correlacionar o manejo diagnóstico terapêutico da Neurotoxoplasmose ao desfecho de morbimortalidade nos indivíduos portadores de Síndrome da Imunodeficiência Humana. Revisão bibliográfica: A toxoplasmose é uma doença provocada pelo Toxoplasma gondii, parasita intracelular semeado pelo Brasil, que pode se instalar e gerar infectividade ao Sistema Nervoso Central (SNC). Em razão da imunodeficiência marcante do dinamismo fisiopatológico do HIV, esses indivíduos são inoperantes no controle da expansão e diferenciação do patógeno possibilitando reativação e menos frequentemente uma infecção aguda com repercussões no SNC como cefaleia, confusão mental, convulsões e alterações de força. Como pilar essencial do diagnóstico ancora-se a história clínica epidemiológica a Ressonância Magnética do Crânio com lesão hipo/isodensa com captação anelar do contraste e efeito de massa exercido. Considerações finais: Nessa perspectiva, com a maior facilidade de acesso à Terapia Antiretroviral atualmente é possível observar, ao longo dos anos, uma queda significativa da incidência de neurotoxoplasmose no Brasil. No entanto, a neurotoxoplasmose ainda é uma causa significativa de morbidade e mortalidade em pacientes imunossuprimidos, sendo importante reconhecer, conseguir diagnosticar e tratar tal patogenia de maneira efetiva, pois o intervalo de tempo entre o diagnóstico e o início do tratamento é determinante na morbimortalidade.
Spinal canal invasion as a predictor of neurological deficit in traumatic vertebral burst fractures
Authors: Montes-Aguilar Oscar Josue , Alaniz-Sida Karmen Karina , Dufoo-Olvera Manuel , Ladewig-Bernaldez Guillermo Ivan , Oropeza-Oropeza Edith , Gómez-Flores Gerson , Pérez-Rios Jeasson Javier , Miguel-Zambrano Alejandro , Ochoa-González Maurilio Vicente , Tirado-Ornelas Héctor Alonso 
Published: September 2022

Background:
This study correlated the extent of spinal canal compression from retropulsed traumatic burst cervical, thoracic, and lumbar spine fractures with the severity of neurological dysfunction.

Methods:
One hundred and sixty-nine patients with cervical, thoracic, or lumbar sub-axial traumatic burst fractures were seen in an emergency department from 2019 to 2021; 79.3% were men, averaging 37 years of age. The lumbar spine was most frequently involved (42%), followed by the thoracic (36.1%) and cervical (21.9%) levels. The extent of spinal canal compression was quantitated utilizing Hashimoto’s method, and correlated with patients’ extent of neurological injury based on their American Spinal Injury Association scores.

Results:
There was a positive correlation between the extent of cervical and thoracic spinal cord compression due to retro pulsed burst fragments and the severity of the patients’ neurological deficits, but this was not true for the lumbar spine.

Conclusion:
The extent of spinal cord compression from retropulsed cervical and thoracic traumatic burst fractures was readily correlated with the severity of patients’ neurological dysfunction. However, there was no such correlation between the extent of cauda equina compression from retropulsed lumbar burst fractures and the severity of their cauda equina syndromes.
Caracterización del impacto de la pandemia por Covid- 19 en el servicio de cirugía Gineco-oncológica en dos instituciones de III nivel en Colombia
Authors: Caicedo Páez Lina Maria , Serranoi Oscar , Santana Diana , Bonilla Carlos , Rodríguez Martha , Luna Joaquín 
Published: September 2022

Introducción: La declaración de una pandemia de COVID-19 ha generado múltiples cambios en el manejo de los pacientes con cáncer por temor al contagio en las instituciones de salud, lo que llevo a posponer el inicio de tratamientos y prolongar tiempos de seguimiento, sin conocer el impacto en el pronóstico y supervivencia. Este estudio describe la experiencia de un grupo de ginecología oncológica, que continuó el programa quirúrgico sin variaciones en el volumen de pacientes o tiempos de programación.
Métodos: se realizó un estudio de cohorte observacional retrospectivo, incluyó a 156 pacientes que se les realizo cirugía programada en el Servicio de Ginecología Oncológica, del 15 de marzo al 15 de julio de 2020. Se describieron las tasas de infección sintomática y mortalidad por COVID 19, con un seguimiento hasta 30 días después del procedimiento quirúrgico en pacientes y en el grupo de cirujanos
Resultados: Se reclutaron 156 pacientes, se analizaron 154 casos. La edad media de los pacientes fue 50 años (21,4% mayores de 60 años). El 13,4% eran obesos. El 96,7% recibieron anestesia general. El diagnóstico oncológico más frecuente fue carcinoma de cuello uterino en 19,4% de los casos. El abordaje quirúrgico se realizó de forma abierta en el 69% de los casos y laparoscópica en el 20%. La complicación mas frecuente fue la infección del sitio quirúrgico (1,9%).
Conclusión: Es posible continuar con un manejo oncológico quirúrgico oportuno y seguro, sin incrementar las tasas de complicaciones y mortalidad por COVID-19 en los pacientes y el grupo quirúrgico.
Efficacy of Combination of Meropenem with Gentamicin, and Amikacin against Resistant E. coli Isolated from Patients with UTIs: in vitro Study
Authors: A. abdul khaleq Maysaa , H. Abd Abdulkareem , A. dhahi Maysaa 
Published: September 2022

Seventy five E. coli isolates were collected from urine of patients with urinary tract infections in AL-Kadhimia and AL-Yarmook teaching hospitals in Baghdad for a period between 22/11/2009 to 15/3/2010, from these samples twenty five isolates were selected according to their pattern of the highest resistance as these showing multi-drug resistances and tested to specify their minimum inhibitory concentration for (meropenem, gentamicin and amikacin), meropenem was found having the lowest MIC comparing with others. This study also includes in vitro effects of various combinations of three types of antimicrobials (meropenem, gentamicin and amikacin) against twenty five E. coli isolates.Among combinations the combination of meropenem with the other types of antimicrobials showed high synergistic effect when 1/4+1/4 MIC for each antimicrobial were used. While combinations of amikacin with gentamicin in some isolates showed additive effect when 1/2+1/2 MIC for each antimicrobial were used. The plasmid profile for the twenty five E. coli isolates were studied using Pure Yeild â„¢ plasmid Miniprep system- Cat.# A1220 â€“ Promega- USA. In order to determined the presence of plasmid for antimicrobials resistance.
Synthesis and Preliminary Antimicrobial Study of 2-Amino-5-Mercapto-1,3,4-Thiadiazole Derivatives
Authors: A. Ameen Husam , J. Qasir Ahlam 
Published: September 2022

Nitrogen heterocycles are of a special interest because they constitute an important class of natural and non natural products, many of which exhibit useful biological activities.Among these nitrogen heterocycles are 1, 3, 4-thiadiazole containing compounds. The therapeutic effects of these derivatives have been well studied for a number of pathological conditions including inflammation, pain, or hypertension. Moreover, synthesis of thiadiazoles has attracted wide-spread attention due to their diverse applications as antibacterial, anticancer, antifungal anti-inflammatory and antidepressant agents.According to this informationâ€™s new derivatives of 1, 3, 4-thiadiazole were designed and synthesized and in the hope of having some activities as antibacterial and antifungal. These are:

4-(((5-mercapto-1,3,4-thiadiazol-2-yl)imino)methyl)-2-methoxyphenol.
Tert-butyl (1- ((2- ((5- (methylsulfonyl) -1,3,4- thiadiazol-2-yl) amino) -2- oxoethyl)amino) -1-oxopropan - 2-yl) carbamate.

Key words: 1, 3, 4-thiadiazole, biological activity, peptides, Schiff base.
The Effects of Aloe vera Gel, Sesame Oil and Camphor Oil on Pseudomonas aeruginosa Isolated from Burnt Patients
Authors: T. Flayyih May , Q. Majeed Raghad 
Published: September 2022

Three isolates of P. aeruginosa were isolated from burnt patients. The ability of these isolates for adhesion and formation of slime layer were tested, the result showed that all isolates were able to adherence on the smooth surface. The sensitivity of P. aeruginosa isolates for antibiotics were tested , all isolates were sensitive to Gentamycin, Piperacillin and Amikacin Ciprofloxacin, and resist to Tetracyclin, Amoxicillin, Cephalexine , Ceftriaxone. Ciprofloxacin and Amikacin were found effective against P. aeruginosa isolates with MIC values of 3.8 Î¼g/ ml for Ciprofloxacin and 0.244 Î¼g/ ml for Amikacin The antibacterial effect of Different concentrations of Aloe vera gel, Sesame Oil and Camphor Oil against P. aeruginosa were determined, Camphor was highly effective with Concentration inhibit bacteria value of 10% followed by Sesame Oil (20%) and Aloe vera gel (>75%). The combinations of Aloe vera gel, Sesame Oil and Camphor Oil and antibiotics (Ciprofloxacin and Amikacin ) showed that the efficacy of the two antibiotics (Ciprofloxacin and Amikacin ) against P.aeroginosa isolates was improved in the presence of Aloe vera gel, Sesame Oil and Camphor Oil.
Key words : Aloe vera gel, Sesame Oil , Camphor Oil , Pseudomonas aeruginosa.