bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct conversion of N2 and O2: status, challenge and perspective
Authors: Li Di , Zan Lingxing , Chen Shiming , Shi Zhang-Jie , Chen Ping , Xi Zhenfeng , Deng Dehui 
Published: March 2022

Abstract
As key components of air, nitrogen (N2) and oxygen (O2) are the vital constituents of lives. Synthesis of NO2, and C–N–O organics direct from N2 and O2, rather than from an intermediate NH3 (known as the Haber–Bosch process), is tantalizing. However, the extremely strong N≡N triple bond (945 kJ mol–1) and the nonpolar stable electron configuration of dinitrogen lead to its conversion being extensively energy demanding. The further selective synthesis of high-value C–N–O organics directly from N2, O2 and C-containing molecules is attractive yet greatly challenging from both scientific and engineering perspectives. Enormous efforts have been dedicated to the direct conversion of N2 and O2 via traditional and novel techniques, including thermochemical, plasma, electrochemical, ultrasonic and photochemical conversion. In this review, we aim to provide a thorough comprehension of the status and challenge of the direct conversion of N2, O2 and C-containing molecules (particularly N2 and O2). Moreover, we will propose some future perspectives to stimulate more inspiration from the scientific community to tackle the scientific and engineering challenges.
Systematic review of carotenoid concentrations in human milk and infant blood
Authors: Zaidi Yusuf , Stroh Rachel , Moran Nancy E 
Published: March 2022

AbstractContextDietary carotenoid intake is associated with vitamin A status and healthy visual and cognitive function in early life. To date, however, only limited population-level data on the concentrations of carotenoids in human milk or infant blood have been available to assess the dietary exposure of infants to carotenoids.ObjectiveThis systematic review seeks to define worldwide carotenoid concentrations in human milk and infant blood.Data SourcesThe PubMed, Embase, and Web of Science databases were searched for original research articles published before February 2021.Data ExtractionDietary carotenoid concentrations in human milk and in blood plasma or serum from healthy infants (≤1 year of age), along with study location, infant age, and lactation stage, were extracted. Means and 95%CIs were analyzed within and across variables.Data AnalysisPublications on carotenoid concentrations in infant blood (47 publications, n = 4553 unique individuals) and human milk (65 publications, n = 2871 unique individuals) described populations from 22 and 31 countries, respectively. Carotenoid species concentrations ranged from 0.3 to 20 µg/dL in blood and from 0.1 to 30 µg/dL in human milk, with carotenoid concentrations generally decreasing in milk across lactation stages and increasing in blood with infant age.ConclusionConcentrations of the major dietary carotenoids—β-carotene, lycopene, lutein, β-cryptoxanthin, zeaxanthin, and α-carotene—have been reported in both infant blood and human milk across infant ages and lactation stages, with β-carotene, lutein, and lycopene tending to be more abundant than other carotenoids. Despite heterogeneous amounts of data available for each outcome, infants worldwide are exposed to a variety of dietary carotenoids. The estimates of dietary carotenoids in human milk and infant blood can facilitate the interpretation of future studies and the design of nutritionally relevant experiments on dietary carotenoids and infant health.
The effect of plant-based diets on meta-inflammation and associated cardiometabolic disorders: a review
Authors: Escalante-Araiza Fabiola , Rivera-Monroy Germán , Loza-López Cristina E , Gutiérrez-Salmeán Gabriela 
Published: March 2022

Abstract
Meta-inflammation is a chronic, low-grade state of inflammation secondary to cardiometabolic disorders related to overweight (OW), obesity (OB), consumption of poor nutritional and hypercaloric diets with excessive amounts of lipids and sugars. It can lead to severe metabolic and cardiovascular diseases. Several studies have reported that plant-based diets (PBDs) can be a useful non-pharmacological therapeutic tool for the prevention and treatment of meta-inflammation, since it has been reported that patients following PBDs have lower serum levels of proinflammatory markers, a reduction in weight, and an overall healthier lipid profile. In this review, we describe the pathophysiology of meta-inflammation, the growing worldwide trend toward adoption of PBDs, and how PBDs act to reduce meta-inflammation.
Dolutegravir Monotherapy as Maintenance Strategy: A Meta-Analysis of Individual Participant Data From Randomized Controlled Trials
Authors: Fournier Anna L , Hocqueloux Laurent , Braun Dominique L , Metzner Karin J , Kouyos Roger D , Raffi François , Briant Anaïs R , Martinez Esteban , De Lazzari Elisa , Negredo Eugenia , Rijnders Bart , Rokx Casper , Günthard Huldrych F , Parienti Jean-Jacques 
Published: March 2022

Abstract

Background
Dolutegravir monotherapy (DTG-m) results in virological failure (VF) in some people with human immunodeficiency virus (PWH). We sought to identify the independent factors associated with the risk of VF and to explore the effect size heterogeneity between subgroups of PWH enrolled in DTG-m trials.

Methods
We searched for randomized clinical trials (RCTs) evaluating DTG-m versus combined antiretroviral therapy (cART) among PWH virologically controlled for at least 6 months on cART. We performed an individual participant data meta-analysis of VF risk factors and quantified their explained heterogeneity in random-effect models. Definition of VF was a confirmed plasma human immunodeficiency virus (HIV)-1 ribonucleic acid (RNA) &gt;50 copies/mL by week 48.

Results
Among 416 PWH from 4 RCTs, DTG-m significantly increased the risk of VF (16 of 227 [7%] versus 0 of 189 for cART; risk difference 7%; 95% confidence interval [CI], 1%–2%; P = .02; I2 = 51%). Among 272 participants exposed to DTG-m, VF were more likely in participants with the following: first cART initiated ≥90 days from HIV acute infection (adjusted hazard ratio [aHR], 5.16; 95% 95% CI, 1.60–16.65), CD4 T cells nadir &lt;350/mm3 (aHR, 12.10; 95% CI, 3.92–37.40), HIV RNA signal at baseline (aHR, 4.84; 95% CI, 3.68–6.38), and HIV-deoxyribonucleic acid (DNA) copy number at baseline ≥2.7 log/106 peripheral blood mononuclear cells (aHR, 3.81; 95% CI, 1.99–7.30). Among these independent risk factors, the largest effect size heterogeneity was found between HIV DNA subgroups (I2 = 80.2%; P for interaction = .02).

Conclusions
Our study supports the importance of a large viral reservoir size for explaining DTG-m simplification strategy failure. Further studies are needed to link size and genetic diversity of the HIV-1 reservoir.
Autophosphorylation Inhibits RcCDPK1, a Dual-Specificity Kinase that Phosphorylates Bacterial-Type Phosphoenolpyruvate Carboxylase in Castor Oil Seeds
Authors: Kilburn Ryan , Gerdis Suzanne A , She Yi-Min , Snedden Wayne A , Plaxton William C 
Published: March 2022

Abstract
Phosphoenolpyruvate carboxylase (PEPC) is a tightly regulated enzyme that plays a crucial anaplerotic role in central plant metabolism. Bacterial-type PEPC (BTPC) of developing castor oil seeds (COS) is highly expressed as a catalytic and regulatory subunit of a novel Class-2 PEPC heteromeric complex. Ricinus communis Ca2+-dependent protein kinase-1 (RcCDPK1) catalyzes in vivo inhibitory phosphorylation of COS BTPC at Ser451. Autokinase activity of recombinant RcCDPK1 was detected and 42 autophosphorylated Ser, Thr or Tyr residues were mapped via liquid chromatography-tandem mass spectrometry. Prior autophosphorylation markedly attenuated the ability of RcCDPK1 to transphosphorylate its BTPC substrate at Ser451. However, fully dephosphorylated RcCDPK1 rapidly autophosphorylated during the initial stages of a BTPC transphosphorylation assay. This suggests that Ca2+-dependent binding of dephospho-RcCDPK1 to BTPC may trigger a structural change that leads to rapid autophosphorylation and subsequent substrate transphosphorylation. Tyr30 was identified as an autophosphorylation site via LC–MS/MS and immunoblotting with a phosphosite-specific antibody. Tyr30 occurs at the junction of RcCDPK1’s N-terminal variable (NTVD) and catalytic domains and is widely conserved in plant and protist CDPKs. Interestingly, a reduced rate and extent of BTPC transphosphorylation occurred with a RcCDPK1Y30F mutant. Prior research demonstrated that RcCDPK1’s NTVD is essential for its Ca2+-dependent autophosphorylation or BTPC transphosphorylation activities but plays no role in target recognition. We propose that Tyr30 autophosphorylation facilitates a Ca2+-dependent interaction between the NTVD and Ca2+-activation domain that primes RcCDPK1 for transphosphorylating BTPC at Ser451. Our results provide insights into links between the post-translational control of COS anaplerosis, Ca2+-dependent signaling and the biological significance of RcCDPK1 autophosphorylation.
ABI5 binding protein2 inhibits ABA responses during germination without ABA-INSENSITIVE5 degradation
Authors: Lynch Tim , Née Guillaume , Chu Avan , Krüger Thorben , Finkemeier Iris , Finkelstein Ruth R 
Published: March 2022

AbstractOverexpression of ABA-INSENSITIVE5 binding proteins (AFPs) results in extreme ABA resistance of seeds and failure to acquire desiccation tolerance, at least in part through effects on chromatin modification. We tested the hypothesis that AFPs promote germination in Arabidopsis (Arabidopsis thaliana) by also functioning as adapters for E3 ligases that ubiquitinate ABI5, leading to its degradation. Interactions between AFPs and two well-characterized classes of E3 ligases targeting ABI5, DWD HYPERSENSITIVE TO ABA (DWA)s and KEEP ON GOING, were analyzed by yeast two-hybrid, bimolecular fluorescence complementation, and genetic assays. Although weak direct interactions were detected between AFPs and E3 ligases, loss of function for these E3 ligases did not impair ABA-resistance conferred by overexpression of the YFP–AFP2 fusion. Comparison of ABI5 and AFP2 levels in these lines showed that AFP2 accumulation increased during germination, but that ABI5 degradation followed germination, demonstrating that AFP2 overexpression reduces ABA sensitivity, thereby permitting germination prior to ABI5 degradation. Surprisingly, AFP2 overexpression in the dwa1 dwa2 mutant background produced the unusual combination of extreme ABA resistance and desiccation tolerance, creating an opportunity to separate the underlying biochemical characteristics of ABA sensitivity and desiccation tolerance. Our quantitative proteomics analysis identified at least three-fold more differentially accumulated seed proteins than previous studies. Comparison of dry seed proteomes of wild-type or dwa1 dwa2 mutants with or without AFP2 overexpression allowed us to separate and refine the changes in protein accumulation patterns associated with desiccation tolerance independently of ABA sensitivity, or vice versa, to a subset of cold-induced and defense stress-responsive proteins and signaling regulators.
Transcription factor StABI5-like 1 binding to the FLOWERING LOCUS T homologs promotes early maturity in potato
Authors: Jing Shenglin , Sun Xiaomeng , Yu Liu , Wang Enshuang , Cheng Zhengnan , Liu Huimin , Jiang Peng , Qin Jun , Begum Shahnewaz , Song Botao 
Published: March 2022

Abstract
Potato (Solanum tuberosum L.) maturity involves several important traits, including the onset of tuberization, flowering, leaf senescence, and the length of the plant life cycle. The timing of flowering and tuberization in potato is mediated by seasonal fluctuations in photoperiod and is thought to be separately controlled by the FLOWERING LOCUS T-like (FT-like) genes SELF-PRUNING 3D (StSP3D) and SELF-PRUNING 6A (StSP6A). However, the biological relationship between these morphological transitions that occur almost synchronously remains unknown. Here, we show that StABI5-like 1 (StABL1), a transcription factor central to abscisic acid (ABA) signaling, is a binding partner of StSP3D and StSP6A, forming an alternative florigen activation complex and alternative tuberigen activation complex in a 14-3-3-dependent manner. Overexpression of StABL1 results in the early initiation of flowering and tuberization as well as a short life cycle. Using genome-wide chromatin immunoprecipitation sequencing and RNA-sequencing, we demonstrate that AGAMOUS-like and GA 2-oxidase 1 genes are regulated by StABL1. Phytohormone profiling indicates an altered gibberellic acid (GA) metabolism and that StABL1-overexpressing plants are insensitive to the inhibitory effect of GA with respect to tuberization. Collectively, our results suggest that StABL1 functions with FT-like genes to promote flowering and tuberization and consequently life cycle length in potato, providing insight into the pleiotropic functioning of the FT gene.
Hybrid knowledge production and evaluation at the World Bank
Authors: Williams Kate 
Published: March 2022

Abstract
Before problems can be solved, they must be defined. In global public policy, problems are defined in large part by institutions like the World Bank, whose research shapes our collective understanding of social and economic issues. This article examines how research is produced at the World Bank and deemed to be worthwhile and legitimate. Creating and capturing research on global policy problems requires organizational configurations that operate at the intersection of multiple fields. Drawing on an in-depth study of the World Bank research department, this article outlines the structures and technologies of evaluation (i.e., the measurements and procedures used in performance reviews and promotions) and the social and cultural processes (i.e., the spoken and unspoken things that matter) in producing valuable policy research. It develops a theoretically informed account of how the conditions of measurement and evaluation shape the production of knowledge at a dominant multilateral agency. In turn, it unpacks how the internal workings of organizations can shape broader epistemic infrastructures around global policy problems.
The

H

elping

E

veryone

A

chieve

L

ong

T

erm

H

ealth Passport: exploring potential use of the HEALTH Passport in primary and secondary schools
Authors: Bechman Natasha , Thornby John , Brandstatter Emma , Hewitt Des , Patel Vinod 
Published: March 2022

Abstract

Background
Chronic disease is a significant burden on the global population. The Helping Everyone Achieve Long Term Health (HEALTH) Passport is a paper-based approach previously utilized to help adults modify clinical risk factors through lifestyle, which may be effective in improving the long-term health of school-age children. This study investigates the feasibility of in-school use by engaging trainee teachers in primary and secondary education.

Methods
Two hundred and fifty six unique responses were collated to evaluate current teaching of the main health risk factors and HEALTH Passports specifically adapted for schools. Trainees attended workshops with pre- and post-questionnaires used to measure training efficacy and evaluate the Passports’ suitability for in-school use. Narrative analysis of feedback was performed.

Results
Feedback received for both Passports was positive overall. Trainees highlighted the need for the Passports to be further age differentiated. Significantly increased confidence (P &lt; 0.01) in knowledge of exercise, type 2 diabetes, weight and blood pressure was shown. Confidence in smoking, drugs and alcohol knowledge was reduced highlighting the requirement for further teacher training.

Conclusions
The HEALTH Passport has potential as an intervention to improve health literacy in school-age children. Age adaptation is needed with references to weight measures removed. Emotional well-being should be focused on, and data management stringently assessed for child protection.
TNFR2 is critical for TNF-induced rheumatoid arthritis fibroblast-like synoviocyte inflammation
Authors: Suto Takahito , Tosevska Anela , Dalwigk Karolina , Kugler Maximillian , Dellinger Mirjam , Stanic Irena , Platzer Alexander , Niederreiter Birgit , Sevelda Florian , Bonelli Michael , Pap Thomas , Kiener Hans , Okamura Koichi , Chikuda Hirotaka , Aletaha Daniel , Heinz Leonhard X , Karonitsch Thomas 
Published: March 2022

Abstract

Objectives
TNF-induced activation of fibroblast-like synoviocytes (FLS) is a critical determinant for synovial inflammation and joint destruction in RA. The detrimental role of TNF-receptor 1 (TNFR1) has thoroughly been characterized. The contributions of TNFR2, however, are largely unknown. This study was performed to delineate the role of TNFR2 in human FLS activation.

Methods
TNFR2 expression in synovial tissue samples was determined by immunohistochemistry. Expression of TNFR2 was silenced using RNAi or CRISPR/Cas9 technologies. Global transcriptional changes were determined by RNA-seq. QPCR, ELISA and immunoblotting were used to validate RNA-seq results and to uncover pathways operating downstream of TNFR2 in FLS.

Results
TNFR2 expression was increased in RA when compared with OA synovial tissues. In particular, RA-FLS demonstrated higher levels of TNFR2 when compared with OA-FLS. TNFR2 expression in RA-FLS correlated with RA disease activity, synovial T- and B-cell infiltration. TNF and IL1β were identified as inflammatory mediators that upregulate TNFR2 in RA-FLS. Silencing of TNFR2 in RA-FLS markedly diminished the TNF-induced expression of inflammatory cytokines and chemokines, including CXCR3-binding chemokines and the B-cell activating factor TNFSF13B. Immunobiochemical analyses revealed that TNFR2-mediated expression of inflammatory mediators critically depends on STAT1.

Conclusion
Our results define a critical role for TNFR2 in FLS-driven inflammation and unfold its participation in the unresolved course of synovial inflammation in RA.
The role of PET/CT in disease activity assessment in patients with large vessel vasculitis
Authors: Galli Elena , Muratore Francesco , Mancuso Pamela , Boiardi Luigi , Marvisi Chiara , Besutti Giulia , Spaggiari Lucia , Casali Massimiliano , Versari Annibale , Giorgi Rossi Paolo , Salvarani Carlo 
Published: March 2022

Abstract

Objectives
To evaluate the accuracy of PET/CT and of PET vascular activity score (PETVAS) in assessing disease activity and the ability of PETVAS in predicting relapses in a large single-centre cohort of patients with large vessel vasculitis (LVV).

Methods
We conducted a retrospective cohort study of prospectively collected data of consecutive patients diagnosed with LVV who underwent at least one PET/CT scan between 2007 and 2020. The nuclear medicine physician’s interpretation of each PET/CT scan (active/inactive vasculitis) was compared with disease activity clinical judgement (active disease/remission). For each PET/CT scan, the PETVAS score was calculated and its accuracy in assessing disease activity was evaluated. The ability of PETVAS in predicting subsequent relapses was evaluated.

Results
A total of 100 consecutive LVV patients (51 large vessel GCA, 49 Takayasu arteritis) underwent a total of 476 PET/CT scans over a mean follow-up period of 97.5 months. Physician-determined PET/CT grading was able to distinguish between clinically active and inactive LVV with a sensitivity of 60% (95% CI 50.9, 68.7) and specificity of 80.1% (95% CI 75.5, 84.1); the area under the curve (AUC )was 0.70 (95% CI 0.65, 0.75). PETVAS was associated with disease activity, with an age and sex–adjusted odds ratio for active disease of 1.15 (95% CI 1.11, 1.19). A PETVAS ≥10 provided 60.8% sensitivity and 80.6% specificity in differentiating between clinically active and inactive LVV; the AUC was 0.73 (95% CI 0.68, 0.79). PETVAS was not associated with subsequent relapses, with an age and sex–adjusted hazard ratio of 1.04 (95% CI 0.97, 1.11).

Conclusions
The visual PET/CT grading scale and PETVAS had moderate accuracy to distinguish active LVV from remission. PETVAS did not predict disease relapses.
Oxytocin enhances basolateral amygdala activation and functional connectivity while processing emotional faces: preliminary findings in autistic vs non-autistic women
Authors: Procyshyn Tanya L , Lombardo Michael V , Lai Meng-Chuan , Jassim Nazia , Auyeung Bonnie , Crockford Sarah K , Deakin Julia B , Soubramanian Sentil , Sule Akeem , Terburg David , Baron-Cohen Simon , Bethlehem Richard A I 
Published: March 2022

Abstract
Oxytocin is hypothesized to promote social interactions by enhancing the salience of social stimuli. While previous neuroimaging studies have reported that oxytocin enhances amygdala activation to face stimuli in autistic men, effects in autistic women remain unclear. In this study, the influence of intranasal oxytocin on activation and functional connectivity of the basolateral amygdala—the brain’s ‘salience detector’—while processing emotional faces vs shapes was tested in 16 autistic and 21 non-autistic women by functional magnetic resonance imaging in a placebo-controlled, within-subject, cross-over design. In the placebo condition, minimal activation differences were observed between autistic and non-autistic women. However, significant drug × group interactions were observed for both basolateral amygdala activation and functional connectivity. Oxytocin increased left basolateral amygdala activation among autistic women (35-voxel cluster, Montreal Neurological Institute (MNI) coordinates of peak voxel = −22 −10 −28; mean change = +0.079%, t = 3.159, PTukey = 0.0166) but not among non-autistic women (mean change = +0.003%, t = 0.153, PTukey = 0.999). Furthermore, oxytocin increased functional connectivity of the right basolateral amygdala with brain regions associated with socio-emotional information processing in autistic women, but not in non-autistic women, attenuating group differences in the placebo condition. Taken together, these findings extend evidence of oxytocin’s effects on the amygdala to specifically include autistic women and specify the subregion of the effect.
The design of transformative research and innovation policy instruments for grand challenges: The policy-nesting perspective
Authors: Borrás Susana , Schwaag Serger Sylvia 
Published: March 2022

Abstract
Several countries have created policy instruments seeking to direct research and innovation (R&I) toward addressing societal challenges. However, the design of such instruments might not always live up to their proclaimed transformative rationale. The aim of this paper is to examine empirically this matter. In a unique cross country comparison of four Grand Challenge-oriented R&I programs in the Nordic countries, we ask to what extent the design of new policy instruments for grand challenges are nested according to the rationale of transformative R&I policy. The findings show that, while all have individual transformative elements, they only exhibit weak or medium degrees of nesting. At a time of increasing transformative ambition of R&I policies, our findings make an important contribution to understanding and addressing the complexity of designing R&I transformative policy instruments.