bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhizosheath: An adaptive root trait to improve plant tolerance to phosphorus and water deficits?
Authors: Aslam Mehtab Muhammad , Karanja Joseph K. , Dodd Ian C. , Waseem Muhammad , Weifeng Xu 
Published: July 2022

AbstractDrought and nutrient limitations adversely affect crop yields, with below‐ground traits enhancing crop production in these resource‐poor environments. This review explores the interacting biological, chemical and physical factors that determine rhizosheath (soil adhering to the root system) development, and its influence on plant water uptake and phosphorus acquisition in dry soils. Identification of quantitative trait loci for rhizosheath development indicate it is genetically determined, but the microbial community also directly (polysaccharide exudation) and indirectly (altered root hair development) affect its extent. Plants with longer and denser root hairs had greater rhizosheath development and increased P uptake efficiency. Moreover, enhanced rhizosheath formation maintains contact at the root‐soil interface thereby assisting water uptake from drying soil, consequently improving plant survival in droughted environments. Nevertheless, it can be difficult to determine if rhizosheath development is a cause or consequence of improved plant adaptation to dry and nutrient‐depleted soils. Does rhizosheath development directly enhance plant water and phosphorus use, or do other tolerance mechanisms allow plants to invest more resources in rhizosheath development? Much more work is required on the interacting genetic, physical, biochemical and microbial mechanisms that determine rhizosheath development, to demonstrate that selection for rhizosheath development is a viable crop improvement strategy.
The CBL‐CIPK network is involved in the physiological crosstalk between plant growth and stress adaptation
Authors: Mao Jingjing , Mo Zhijie , Yuan Guang , Xiang Haiying , Visser Richard G. F. , Bai Yuling , Liu Haobao , Wang Qian , van der Linden C. Gerard 
Published: July 2022

AbstractPlants have evolved to deal with different stresses during plant growth, relying on complex interactions or crosstalk between multiple signalling pathways in plant cells. In this sophisticated regulatory network, Ca2+ transients in the cytosol ([Ca2+]cyt) act as major physiological signals to initiate appropriate responses. The CALCINEURIN B‐LIKE PROTEIN (CBL)‐CBL‐INTERACTING PROTEIN KINASE (CIPK) network relays physiological signals characterised by [Ca2+]cyt transients during plant development and in response to environmental changes. Many studies are aimed at elucidating the role of the CBL‐CIPK network in plant growth and stress responses. This review discusses the involvement of the CBL‐CIPK pathways in two levels of crosstalk between plant development and stress adaptation: direct crosstalk through interaction with regulatory proteins, and indirect crosstalk through adaptation of correlated physiological processes that affect both plant development and stress responses. This review thus provides novel insights into the physiological roles of the CBL‐CIPK network in plant growth and stress adaptation.
Gene expression of a canopy‐forming kelp, Eisenia bicyclis (Laminariales, Phaeophyceae), under high temperature stress
Authors: Hara Yoshino , Otake Yui , Akita Shingo , Yamazaki Tomokazu , Takahashi Fumio , Yoshikawa Shinya , Shimada Satoshi 
Published: July 2022

SUMMARYTo understand the high temperature stress acclimation of a canopy‐forming kelp, RNA‐seq analysis was performed on cultured Eisenia bicyclis (Kjellman) Setchell (Laminariales, Phaeophyceae), a major component species of kelp forests along the coast of Japan. We established a culture strain from a population at the northernmost distribution limit of this species and measured photosystem II activity in young sporophytes. We found that photosystem II activity was lower at 25°C than at 5–15°C, thus, comparative RNA‐seq analysis was conducted between cultivated young thalli of E. bicyclis at 15°C and 25°C. Comparison with the expression genes revealed 277 up‐regulated genes and 327 down‐regulated genes at 25°C. The most up‐regulated gene was 3‐ketoacyl‐CoA thiolase, which is related to fatty acid degradation. This enzyme has been reported to positively regulate abscisic acid (ABA) signaling in Arabidopsis thaliana (Brassicaceae). In addition, heat shock proteins, ATP synthase, NADH‐dehydrogenase, and L‐ascorbate peroxidase were up‐regulated. The most down‐regulated gene was taurine catabolism dioxygenase TauD/TfdA. Genes related to fatty acid metabolism, photosynthesis, and synthesis of cell wall components were down‐regulated. These results suggest that E. bicyclis may counteract high temperature stress by up‐regulating genes involved in protein stabilization, energy production and antioxidant processes. Conversely, photosynthesis and other metabolic processes may be damaged due to the inability to withstand high temperature stress.
Cultivation of fish ciliate parasites: Progress and prospects
Authors: Li Ming , Bastos Gomes Giana , Zhao Weishan , Hu Guangran , Huang Ke , Yoshinaga Tomoyoshi , Clark Theodore G. , Li Wenxiang , Zou Hong , Wu Shangong , Wang Guitang 
Published: July 2022

AbstractCiliates are considered the most harmful parasites of fish, causing mass mortalities and thus substantial economic losses to the aquaculture and ornamental fish industries. To better elucidate their pathogenic mechanisms and facilitate the development of effective prevention and control strategies, it is essential to apply novel technologies to ensure the continuous and sufficient supply of parasite specimens. This review discusses the latest research progress in the artificial culture of six important parasitic ciliates affecting both freshwater and marine fish globally:Ichthyophthirius multifiliis,Cryptocaryon irritans,Chilodonella(C. hexasticha,C. piscicolaandC. uncinata),Balantidium ctenopharyngodoni, Scuticociliatida gen. spp., andTetrahymenaspp. Methods for in vitro and in vivo culture as well as cryopreservation/preservation (depending on the specific species) of these organisms are described herein. Challenges and future applications of parasite culture are also discussed.
ECB monetary policy and commodity prices
Authors: Aliyev Shahriyar , Kočenda Evžen 
Published: July 2022

AbstractWe assess the impact of ECB monetary policy on global aggregate and sectoral commodity prices over 2001–2019. We employ an SVAR model and separately assess periods before and after the global financial crisis. Our key results indicate that contractionary monetary policy shocks have positive effects on commodity prices during both conventional and unconventional monetary policy periods, indicating the effectiveness of unconventional monetary policy tools. The largest impact is documented on energy (fuel) and food commodities. Our results also suggest that the effect of ECB monetary policy on commodity prices transmits through the exchange rate channel, which influences European market demand.
Effect of Fiber Geometry on Fracture and Fatigue of Composite Hydrogels
Authors: Tang Jingda , Chen Xi , Men Libo , Wang Zhengjin 
Published: July 2022

Abstract
Hydrogel-based biomedical applications are under rapid development. These applications usually demand hydrogels to have high toughness and high fatigue threshold. Recently, various fiber-reinforced composite hydrogels have been developed to meet this challenge. However, the effect of fiber geometry on the fracture and fatigue of composite hydrogels is still elusive. Here, we use a model composite hydrogel to study the influence of fiber width, fiber spacing, and fiber configuration on these properties. It is found that the toughness of the composite hydrogel does not increase monotonically with the fiber width or fiber spacing, but presents a peak. This is because the variation of fiber width and fiber spacing not only affects the volume of fiber in the fracture process zone but also influences the dissipated elastic energy density in that volume, which is affected by the stress concentration. The peak is a consequence of the trade-off between these two factors. Our study further shows that the shape of the fiber network affects the stress concentration in the fiber dramatically, thereby leading to a huge difference in the toughness and fatigue threshold of the composite hydrogels. This work highlights the importance of fiber size as well as the shape of fiber networks on the mechanical properties of composite hydrogels. It may help the design of tough and fatigue-resistant stretchable composite materials.
Optimization of Exhaust Covers in Marine Scrubbers
Authors: Poulsen Mathias , Sørensen Kim , Condra Thomas 
Published: July 2022

Abstract
The number of marine scrubbers installed in industry has been on the rise over the past decade and is expected to continue in the coming years. Therefore, it is essential to ensure that the design of the scrubbers enables as an efficient operation as possible. In this study, an optimization of the exhaust cover inside an in-line scrubber was carried out. The optimization was done by combining a computational fluid dynamics model working on a simplified geometry with the method of feasible directions in order to reduce the pressure loss caused by the exhaust cover. The design is constrained in both height and width of the points making up the exhaust cover to ensure proper drainage of water and to avoid invalid designs. It was found that the optimized design reduced the pressure loss by 42% compared to the initial design. Furthermore, the scalability of the original design was investigated with the same height constraint enforced on the design variables. The result of the scalability analysis showed that the radius of the exhaust cover for the optimal designs scales linearly with the diameter of the scrubber, while the pressure loss was found to increase quadratically as the diameter of the scrubber increases.
Energy Availability Over One Athletic Season: An Observational Study Among Athletes From Different Sports
Authors: Jesus Filipe , Sousa Mónica , Nunes Catarina L. , Francisco Ruben , Rocha Paulo , Minderico Cláudia S. , Sardinha Luís B. , Silva Analiza M. 
Published: July 2022

During the athletic season, changes in body composition occur due to fluctuations in energy expenditure and energy intake. Literature regarding changes of energy availability (EA) is still scarce. The aim was to estimate EA of athletes from nonweight and weight-sensitive sports during the athletic season (i.e., preparatory and competitive phase). Eighty-eight athletes (19.1 ± 4.2 years, 21.8 ± 2.0 kg/m2, 27% females, self-reported eumenorrheic) from five sports (basketball [n = 29]; handball [n = 7]; volleyball [n = 9]; swimming [n = 18]; and triathlon [n = 25]) were included in this observational study. Energy intake and exercise energy expenditure were measured through doubly labeled water (over 7 days and considering neutral energy balance) and metabolic equivalents of tasks, respectively. Fat-free mass (FFM) was assessed through a four-compartment model. EA was calculated as EA = (energy intake − exercise energy expenditure)/FFM. Linear mixed models, adjusted for sex, were performed to assess EA for the impact of time by sport interaction. Among all sports, EA increased over the season: basketball, estimated mean (SE): 7.2 (1.5) kcal/kg FFM, p < .001; handball, 14.8 (2.9) kcal/kg FFM, p < .001; volleyball, 7.9 (2.8) kcal/kg FFM, p = .006; swimming, 8.7 (2.0) kcal/kg FFM, p < .001; and triathlon, 9.6 (2.0) kcal/kg FFM, p < .001. Eleven athletes (12.5%) had clinical low EA at the preparatory phase and none during the competitive phase. During both assessments, triathletes’ EA was below optimal, being lower than basketballers (p < .001), volleyballers (p < .05), and swimmers (p < .001). Although EA increased in all sports, triathlon’s EA was below optimal during both assessments. Risk of low EA might be seasonal and resolved throughout the season, with higher risk during the preparatory phase. However, in weight-sensitive sports, namely triathlon, low EA is still present.
Accuracy of algorithms to identify patients with a diagnosis of major cancers and cancer-related adverse events in an administrative database: a validation study in an acute care hospital in Japan
Authors: Fujiwara Takashi , Kanemitsu Takashi , Tajima Kosei , Yuri Akinori , Iwasaku Masahiro , Okumura Yasuyuki , Tokumasu Hironobu 
Published: July 2022

ObjectivesValidation studies in oncology are limited in Japan. This study was conducted to evaluate the accuracy of diagnosis and adverse event (AE) definitions for specific cancers in a Japanese health administrative real-world database (RWD).Design and settingRetrospective observational validation study to assess the diagnostic accuracy of electronic medical records (EMRs) and claim coding regarding oncology diagnosis and AEs based on medical record review in the RWD. The sensitivity and positive predictive value (PPV) with 95% CIs were calculated.ParticipantsThe validation cohort included patients with lung (n=2257), breast (n=1121), colorectal (n=1773), ovarian (n=216) and bladder (n=575) cancer who visited the hospital between January 2014 and December 2018, and those with prostate cancer (n=3491) visiting between January 2009 and December 2018, who were identified using EMRs.OutcomesKey outcomes included primary diagnosis, deaths and AEs.ResultsFor primary diagnosis, sensitivity and PPV for the respective cancers were as follows: lung, 100.0% (96.6 to 100.0) and 81.0% (74.9 to 86.2); breast, 100.0% (96.3 to 100.0) and 74.0% (67.3 to 79.9); colorectal, 100.0% (96.6 to 100.0) and 80.5% (74.3 to 85.8); ovarian, 89.8% (77.8 to 96.6) and 75.9% (62.8 to 86.1); bladder, 78.6% (63.2 to 89.7) and 67.3% (52.5 to 0.1); prostate, 100.0% (93.2 to 100.0) and 79.0% (69.7 to 86.5). Sensitivity and PPV for death were as follows: lung, 97.0% (84.2 to 99.9) and 100.0% (84.2 to 100.0); breast, 100.0% (1.3 to 100.0) and 100.0% (1.3 to 100.0); colorectal, 100.0% (28.4 to 100.0) and 100.0% (28.4 to 100.0); ovarian, 100.0% (35.9 to 100.0) and 100.0% (35.9 to 100.0); bladder, 100.0% (9.4–100.0) and 100.0% (9.4 to 100.0); prostate, 75.0% (19.4 to 99.4) and 100.0% (19.4 to 100.0). Overall, PPV tended to be low, with the definition based on International Classification of Diseases, 10th revision alone for AEs.ConclusionDiagnostic accuracy was not so high, and therefore needs to be further investigated.Trial registration numberUniversity Hospital Medical Information Network (UMIN) Clinical Trials Registry (UMIN000039345).
Measuring the impact introducing NHS 111 online had on the NHS 111 telephone service and the wider NHS urgent care system: an observational study
Authors: Simpson Rebecca M , Jacques Richard M , Nicholl Jon , Stone Tony , Turner Janette 
Published: July 2022

ObjectivesTo explore what impact introducing the National Health Service (NHS) 111 online service had on the number of phone calls to the NHS 111 telephone service and the NHS urgent care system.DesignObservational study using a dose–response interrupted time series model and random-effects meta- analysis to estimate the average effect.Setting and participantsNHS 111 telephone and online contacts for 18 NHS 111 area codes in England. NHS 111 telephone and online contacts data were collected between October 2010 to December 2019 and January 2018 to December 2019, respectively.Primary and secondary outcome measuresPrimary outcome: the number of triaged calls to the NHS 111 telephone service following the introduction of NHS 111 online. Secondary outcomes: total calls to the NHS 111 telephone service, total number of emergency ambulance referrals or advice to contact 999, total number of advice to attend an emergency department or other urgent care treatment facility, and total number of advice to contact primary care.ResultsFor triaged calls, the overall incidence rate ratio (IRR) per 1000 online contacts was 1.013 (95% CI: 0.996 to 1.029, p=0.127). For total calls, the overall IRR per 1000 online contacts was 1.008 (95% CI: 0.992 to 1.025, p=0.313). For emergency ambulance referrals or advice to contact 999, the overall IRR per 1000 online contacts was 1.067 (95% CI: 1.035 to 1.100, p<0.001). For advice to attend an emergency department or other urgent care treatment facility, the overall IRR per 1000 online contacts is 1.050 (95% CI: 1.010 to 1.092, p=0.014). And finally, for those advised to contact primary care, the overall IRR per 1000 online contacts is 1.051 (95% CI: 1.027 to 1.076, p<0.001).ConclusionsIt was found that the NHS 111 online service has little impact on the number of triaged and total calls, suggesting that the workload for the NHS 111 telephone service has not increased or decreased as a result of introducing NHS 111 online. However, there was evidence to suggest an increase in the overall number of disposition recommendations (ambulance, emergency department and primary care) for NHS 111 telephone and online services combined following the introduction of the NHS 111 online service.
Behavioural intentions in response to a potential menthol cigarette sales ban: a survey examining smokers in Washington, DC public housing
Authors: Dearfield Craig T , Horn Kimberly , Crandell Ian , Bernat Debra H 
Published: July 2022

ObjectivesLocal, national and international policies are being proposed to ban the sale of menthol-flavoured tobacco products. With more bans being implemented, it is increasingly important to understand reactions to these bans among smokers of low socioeconomic status. This study examined public housing residents’ behavioural intentions if menthol-flavoured cigarettes were no longer sold.Setting15 District of Columbia Housing Authority properties between March 2019 and March 2021.Participants221 District of Columbia Housing Authority residents ages 18–80 years who reported smoking menthol cigarettes (83.3% African-American/black).Primary and secondary outcomesCigarette quitting and switching intentions due to a hypothetical menthol-flavoured cigarette sales ban.ResultsNearly one-half (48.0%) of residents said they intended to quit cigarette use if menthol-flavoured products were no longer sold, while 27.2% were unsure if they would quit, and 24.9% reported they would not quit. Older residents (OR 0.94 per year, 95% CI 0.91 to 0.97), senior/disabled building versus family building residents (OR 0.50, 95% CI 0.25 to 0.97), those who smoked within 30 min of waking (OR 0.48, 95% CI 0.23 to 0.98) and daily smokers (OR 0.42, 95% CI 0.21 to 0.84) had lower odds of reporting quit intentions associated with a menthol ban. Of those not intending to quit, 40.7% reported they would switch to non-menthol cigarettes, 20.4% to another non-menthol product, 13.0% to menthol e-cigarettes and 20.4% to another menthol product.ConclusionsResults suggest banning the sale of menthol-flavoured products has the potential to impact cigarette smoking cessation. Nearly three-quarters of smokers in public housing indicated a possibility of quitting smoking because of a menthol cigarette ban. Bans that include all flavours in all tobacco products may be most effective for facilitating overall tobacco cessation.
Barriers and facilitators to implementation of a home-based cardiac rehabilitation programme for patients with heart failure in the NHS: a mixed-methods study
Authors: Daw Paulina , Wood Grace E R , Harrison Alexander , Doherty Patrick J , Veldhuijzen van Zanten Jet J C S , Dalal Hasnain M , Taylor Rod S , van Beurden Samantha B , McDonagh Sinead T J , Greaves Colin J 
Published: July 2022

ObjectivesThis study aimed to identify barriers to, and facilitators of, implementation of the Rehabilitation EnAblement in CHronic Heart Failure (REACH-HF) programme within existing cardiac rehabilitation services, and develop and refine the REACH-HF Service Delivery Guide (an implementation guide cocreated with healthcare professionals). REACH-HF is an effective and cost-effective 12-week home-based cardiac rehabilitation programme for patients with heart failure.Setting/participantsIn 2019, four early adopter ‘Beacon Sites’ were set up to deliver REACH-HF to 200 patients. In 2020, 5 online REACH-HF training events were attended by 85 healthcare professionals from 45 National Health Service (NHS) teams across the UK and Ireland.DesignOur mixed-methods study used in-depth semi-structured interviews and an online survey. Interviews were conducted with staff trained specifically for the Beacon Site project, identified by opportunity and snowball sampling. The online survey was later offered to subsequent NHS staff who took part in the online REACH-HF training. Normalisation Process Theory was used as a theoretical framework to guide data collection/analysis.ResultsSeventeen healthcare professionals working at the Beacon Sites were interviewed and 17 survey responses were received (20% response rate). The identified barriers and enablers included, among many, a lack of resources/commissioning, having interest in heart failure and working closely with the clinical heart failure team. Different implementation contexts (urban/rural), timing (during the COVID-19 pandemic) and factors outside the healthcare team/system (quality of the REACH-HF training) were observed to negatively or positively impact the implementation process.ConclusionsThe findings are highly relevant to healthcare professionals involved in planning, delivering and commissioning of cardiac rehabilitation for patients with heart failure. The study’s main output, a refined version of the REACH-HF Service Delivery Guide, can guide the implementation process (eg, designing new care pathways) and provide practical solutions to overcoming common implementation barriers (eg, through early identification of implementation champions).
Mapping spreading depolarisations after traumatic brain injury: a pilot clinical study protocol
Authors: Cramer Samuel W , Pino Isabela Peña , Naik Anant , Carlson Danielle , Park Michael C , Darrow David P 
Published: July 2022

IntroductionCortical spreading depolarisation (CSD) is characterised by a near-complete loss of the ionic membrane potential of cortical neurons and glia propagating across the cerebral cortex, which generates a transient suppression of spontaneous neuronal activity. CSDs have become a recognised phenomenon that imparts ongoing secondary insults after brain injury. Studies delineating CSD generation and propagation in humans after traumatic brain injury (TBI) are lacking. Therefore, this study aims to determine the feasibility of using a multistrip electrode array to identify CSDs and characterise their propagation in space and time after TBI.Methods and analysisThis pilot, prospective observational study will enrol patients with TBI requiring therapeutic craniotomy or craniectomy. Subdural electrodes will be placed for continuous electrocorticography monitoring for seizures and CSDs as a research procedure, with surrogate informed consent obtained preoperatively. The propagation of CSDs relative to structural brain pathology will be mapped using reconstructed CT and electrophysiological cross-correlations. The novel use of multiple subdural strip electrodes in conjunction with brain morphometric segmentation is hypothesised to provide sufficient spatial information to characterise CSD propagation across the cerebral cortex and identify cortical foci giving rise to CSDs.Ethics and disseminationEthical approval for the study was obtained from the Hennepin Healthcare Research Institute’s ethics committee, HSR 17-4400, 25 October 2017 to present. Study findings will be submitted for publication in peer-reviewed journals and presented at scientific conferences.Trial registration numberNCT03321370.