bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ablation of the miRNA cluster 24 in cartilage and osteoblasts impairs bone remodeling
Authors: Georgieva Veronika S. , Bluhm Björn , Probst Kristina , Zhu Mengjie , Heilig Juliane , Niehoff Anja , Brachvogel Bent 
Published: June 2022

AbstractMicroRNAs (miRNAs) post-transcriptionally regulate cartilage and bone development and function, however, only few miRNAs have been described to play a role for cartilage to bone transition in vivo. Previously, we showed that cartilage-specific deletion of the Mirc24 cluster in newborn male mice leads to impaired growth plate cartilage development due to increased RAF/MEK/ERK signaling and affects the stability of the cartilage extracellular matrix on account of decreased SOX6 and SOX9 and increased MMP13 levels. Here, we studied how Mirc24 cluster inactivation in cartilage and osteoblasts leads to an increased bone density associated with defects in collagen remodeling in trabecular bone. No changes in osteoblast distribution were observed, whereas the number of osteoclasts was reduced and TRAP activity in osteoclasts decreased. Surprisingly, an increased level of cluster-encoded miR-322 or miR-503 raises Rankl gene expression and inactivation of the cluster in chondrocytes reduces Rankl expression. These results suggest that the Mirc24 cluster regulates Rankl expression in chondrocytes at the chondro-osseous border, where the cluster is mainly expressed to modulate osteoclast formation, bone remodeling and bone integrity.
A high-throughput study of visceral organs in CT-scanned pigs
Authors: Nordbø Øyvind , Sagevik Rune , Kongsro Jørgen , Mikkelsen Kevin , Gjuvsland Arne B. , Gaustad Ann-Helen , Olsen Dan , Remme Espen W. , Grindflek Eli 
Published: June 2022

AbstractIt has been debated whether intensive selection for growth and carcass yield in pig breeding programmes can affect the size of internal organs, and thereby reduce the animal’s ability to handle stress and increase the risk of sudden deaths. To explore the respiratory and circulatory system in pigs, a deep learning based computational pipeline was built to extract the size of lungs and hearts from CT-scan images. This pipeline was applied on CT images from 11,000 boar selection candidates acquired during the last decade. Further, heart and lung volumes were analysed genetically and correlated with production traits. Both heart and lung volumes were heritable, with h2 estimated to 0.35 and 0.34, respectively, in Landrace, and 0.28 and 0.4 in Duroc. Both volumes were positively correlated with lean meat percentage, and lung volume was negatively genetically correlated with growth (rg = − 0.48 ± 0.07 for Landrace and rg = − 0.44 ± 0.07 for Duroc). The main findings suggest that the current pig breeding programs could, as an indirect response to selection, affect the size of hearts- and lungs. The presented methods can be used to monitor the development of internal organs in the future.
Ladder of Eckhaus instabilities and parametric conversion in chi(2) microresonators
Authors: Puzyrev Danila N. , Skryabin Dmitry V. 
Published: June 2022

AbstractLow loss microresonators have revolutionised nonlinear and quantum optics over the past decade. In particular, microresonators with the second order, chi(2), nonlinearity have the advantages of broad spectral tunability and low power frequency conversion. Recent observations have highlighted that the parametric frequency conversion in chi(2) microresonators is accompanied by stepwise changes in the signal and idler frequencies. Therefore, a better understanding of the mechanisms and development of the theory underpinning this behaviour is timely. Here, we report that the stepwise frequency conversion originates from the discrete sequence of the so-called Eckhaus instabilities. After discovering these instabilities in fluid dynamics in the 1960s, they have become a broadly spread interdisciplinary concept. Now, we demonstrate that the Eckhaus mechanism also underpins the ladder-like structure of the frequency tuning curves in chi(2) microresonators.
Success of post-fire plant recovery strategies varies with shifting fire seasonality
Authors: Tangney Ryan , Paroissien Ruby , Le Breton Tom D. , Thomsen Alexandria , Doyle Chantelle A. T. , Ondik Mercedes , Miller Russell G. , Miller Ben P. , Ooi Mark K. J. 
Published: June 2022

AbstractWildfires are increasing in size and severity and fire seasons are lengthening, largely driven by climate and land-use change. Many plant species from fire-prone ecosystems are adapted to specific fire regimes corresponding to historical conditions and shifts beyond these bounds may have severe impacts on vegetation recovery and long-term species persistence. Here, we conduct a meta-analysis of field-based studies across different vegetation types and climate regions to investigate how post-fire plant recruitment, reproduction and survival are affected by fires that occur outside of the historical fire season. We find that fires outside of the historical fire season may lead to decreased post-fire recruitment, particularly in obligate seeding species. Conversely, we find a general increase in post-fire survival in resprouting species. Our results highlight the trade-offs that exist when considering the effects of changes in the seasonal timing of fire, an already present aspect of climate-related fire regime change.
Enhancement on charge transfer properties of Cu12Sb4S13 quantum dots hole transport materials by surface ligand modulation in perovskite solar cells
Authors: Long Chengtang , Peng Zhuoyin , Huang Jincheng , Wang Yue , Luo Wen , Fu Yilong , Chen Jianlin , Chen Jian 
Published: June 2022

A two-step surface ligand-exchange process has been introduced for Cu12Sb4S13 QDs hole transport materials, which effectively enhanced the photovoltaic conversion efficiency of perovskite solar cells from 14.65% to 15.43%.
Gaussian guided IoU: A better metric for balanced learning on object detection
Authors: Gou Lijun , Wu Shengkai , Yang Jinrong , Yu Hangcheng , Li Xiaoping 
Published: June 2022

AbstractMost anchor‐based detectors use intersection over union (IoU) to assign targets to anchors during training. However, IoU did not pay enough attention to the proximity of the anchor's centre to the centre of the truth box, resulting in two issues: (1) the most slender objects were given just one anchor, resulting in insufficient supervision information for slender objects during training; (2) IoU cannot accurately represent the degree of alignment between the feature's receptive field at the anchor's centre and the object. As a result, some features with good alignment degrees are missing, while others with poor alignment degrees are used, reducing the model's localisation accuracy. To address these issues, we first created a Gaussian Guided IoU (GGIoU), which prioritises the proximity of the anchor's centre to the truth box's centre. We then proposed GGIoU‐balanced learning methods, including GGIoU‐guided assignment strategy and GGIoU‐balanced localisation loss. This method can assign multiple anchors to each slender object, favouring features that are well‐aligned with the objects during the training process. A large number of experiments show that GGIoU‐balanced learning can solve the aforementioned problems and significantly improve the detection model's performance.
Analysis and research on the sensitivity of current protection based on the planning and design of distribution networks
Authors: Ge Qun , Xing Jiaqi , Liu Siyuan , Li Zaiqiang 
Published: June 2022

AbstractThe safe and reliable operation of a distribution network (DN) is closely related to the development of national economy and the improvement of people's living standards. At present, the design principle of a distribution network is still based on a primary system and primary equipment, without considering the relay protection on the secondary side, which is an important guarantee for the safe and reliable operation of a power grid. The authors theoretically proved the influence of network structures on the main protection (instantaneous overcurrent protection and time‐delay instantaneous overcurrent protection) of conventional stage‐type current protection, and main influencing factors are analysed and verified in an example. The result shows that reasonable planning and design are an important premise for overcurrent protection to meet sensitivity requirements, and it shows a theoretical and practical guiding significance for the planning of a distribution network.
Distributed lossless coding system based on cloud computing in video transcoding for MRI and neuroimaging
Authors: Li Ying , Shabaz Mohammad 
Published: June 2022

AbstractA distributed lossless coding system based on cloud computing is proposed to increase the video transcoding capacity of the new media interactive broadcasting project in the research department. Distributed transcoding is carried out by using the programming ideas of two Hadoop cores: HDFS (Hadoop distributed file system) and map reduce. At the same time, the specific process of distributed transcoding is introduced and designed in detail. The experimental results show that the efficiency of the distributed transcoding scheme is greatly improved. The results show that this experiment selects the 32 m with the best segment size as the benchmark, and calculates that the distributed transcoding time is 60.3% higher than the traditional single machine transcoding. This improvement significantly improves the transcoding efficiency, improves the experience effect of video service, and adapts to the trend of the rapid development of new media in the current era. The design of the system architecture not only greatly improves the transcoding efficiency, but also saves the transcoding time.
Two Siblings Showing a Mild Phenotype of Joubert Syndrome with a Specific CEP290 Variant
Authors: Uda Daisuke , Kondo Hidehito , Tanda Koichi , Kizaki Zenro , Nishida Masashi , Dai Hongmei , Itoh Masayuki 
Published: June 2022

AbstractJoubert syndrome (JS) is a genetic neurodevelopmental disorder characterized by lower brainstem dysplasia and cerebellar vermis agenesis termed molar tooth sign (MTS), psychomotor retardation, abnormal respiratory pattern in infancy, and oculomotor abnormalities. Arima syndrome (AS), which is a severe form of JS, is characterized by severe psychomotor retardation, congenital visual impairment, progressive renal dysfunction, and lower brainstem dysplasia from early infancy. Numerous patients with AS expire in early childhood. Recently, c.6012–12T> A in the CEP290 gene was reported as a specific variant of AS. Herein, we report the cases of two siblings showing a phenotype of JS with compound heterozygous mutations (c.6012–12T > A / c.5924delT) in the CEP290 gene. The older sister (aged 19 years) had hypotonia, hypertelorism, and anteverted nares since birth. As a neonate, she developed a transient abnormal respiratory pattern and nystagmus, and brain magnetic resonance imaging (MRI) showed MTS. The younger sister (aged 13 years) exhibited mild hypotonia and pendular nystagmus as a neonate; MRI revealed MTS. Both sisters had psychomotor retardation, oculomotor dysfunction, and bilateral renal cysts with normal renal function. They can walk and have simple conversation. They do not meet the diagnostic criteria for AS, and their symptoms were milder than those of previously reported cases with this specific mutation. This report indicates the expanding spectrum of the CEP290 variant.
Palladium/Norbornene-Cocatalyzed Three-Component Synthesis of ortho-Acylated Benzonitriles
Authors: Wang Liqin , Song Guangjun , Yu Xinhong , Chen Niangen , Li Cuiqing , Wu Qiufang , Qin Jiaxin 
Published: June 2022

AbstractA palladium/norbornene-cocatalyzed Catellani-type ortho-C–H-acylation/ipso-cyanation of iodoarenes is developed via a three-component approach using safe and low-toxic sodium carboxylates as the acyl sources and CuCN as the CN source, affording the desired products in yields of 50–94%. Without introducing a directing group on the substrate, a catalytic amount of norbornene and palladium can regioselectively activate the ortho-C–H bonds of iodoarenes. The reaction exhibits good functional group tolerance and works well on gram-scale. In addition, this transformation allows rapid and convenient access toward isoindolinones, 1,4-dicarbonyl compounds and ortho-aminated benzonitriles by manipulation of the cyano and carbonyl groups.
Diagnostic endoscopic submucosal dissection for colorectal lesions with suspected deep invasion
Authors: Patenotte Adrien , Yzet Clara , Wallenhorst Timothée , Subtil Fabien , Leblanc Sarah , Schaefer Marion , Walter Thomas , Lambin Thomas , Fenouil Tanguy , Lafeuille Pierre , Chevaux Jean-Baptiste , Legros Romain , Rostain Florian , Rivory Jérôme , Jacques Jérémie , Lépilliez Vincent , Pioche Mathieu 
Published: June 2022

Abstract
Background Endoscopic submucosal dissection (ESD) is potentially a curative treatment for T1 colorectal cancer under certain conditions. The aim of this study was to evaluate the feasibility and effectiveness of ESD for lesions with a suspicion of focal deep invasion.
Methods In this retrospective multicenter study, consecutive patients with colorectal neoplasia displaying a focal (< 15 mm) deep invasive pattern (FDIP) that were treated by ESD were included. We excluded ulcerated lesions (Paris III), lesions with distant metastasis, and clearly advanced tumors (tumoral strictures).
Results 124 patients benefited from 126 diagnostic dissection attempts for FDIP lesions. Dissection was feasible in 120/126 attempts (95.2 %) and, where possible, the en bloc and R0 resection rates were 95.8 % (115/120) and 76.7 % (92/120), respectively. Thirty-three resections (26.2 %) were for very low risk tumors, so considered curative, and 38 (30.2 %) were for low risk lesions. Noncurative R0 resections were for lesions with lymphatic or vascular invasion (LVI; n = 8), or significant budding (n = 9), and LVI + budding combination (n = 4).
Conclusion ESD is feasible and safe for colorectal lesions with an FDIP ≤ 15 mm. It was curative in 26.6 % of patients and could be a valid option for a further 30.6 % of patients with low risk T1 cancers, especially for frail patients with co-morbidities.
Testing for Lupus Anticoagulants
Authors: Moore Gary W. 
Published: June 2022

AbstractLupus anticoagulant (LA) is one of the three criteria antiphospholipid antibodies (aPLs) employed in classification, and by default diagnosis, of antiphospholipid syndrome (APS). Detection of LA is not via calibrated assays but is based on functional behavior of the antibodies in a medley of coagulation assays. A prolonged clotting time in a screening test is followed by demonstration of phospholipid dependence and inhibitory properties in confirmatory and mixing tests, respectively, which are modifications of the parent screening test. Complications arise because no single screening test is sensitive to every LA, and no test is specific for LA, because they are prone to interference by other causes of elevated clotting times. Several screening tests are available but the pairing of dilute Russell's viper venom time (dRVVT) with LA-sensitive activated partial thromboplastin time (aPTT) is widely used and recommended because it is proven to have good detection rates. Nonetheless, judicious use of other assays can improve diagnostic performance, such as dilute prothrombin time to find LA unreactive with dRVVT and aPTT, and the recently validated Taipan snake venom time with ecarin time confirmatory test that are unaffected by vitamin K antagonist and direct factor Xa inhibitor anticoagulation. Expert body guidelines and their updates have improved harmonization of laboratory practices, although some issues continue to attract debate, such as the place of mixing tests in the medley hierarchy, and areas of data manipulation such as assay cut-offs and ratio generation. This article reviews current practices and challenges in the laboratory detection of LA.
Hirschsprung-Associated Enterocolitis: Transformative Research from Bench to Bedside
Authors: Zhang Zhen , Li Bo , Jiang Qian , Li Qi , Pierro Agostino , Li Long 
Published: June 2022

AbstractHirschsprung disease (HSCR) is a congenital disease that is characterized by the absence of intrinsic ganglion cells in the submucosal and myenteric plexuses of the distal colon and is the most common cause of congenital intestinal obstruction. Hirschsprung-associated enterocolitis (HAEC) is a life-threatening complication of HSCR, which can occur either before or after surgical resection of the aganglionic bowel. Even though HAEC is a leading cause of death in HSCR patients, its etiology and pathophysiology remain poorly understood. Various factors have been associated with HAEC, including the mucus barrier, microbiota, immune function, obstruction of the colon, and genetic variations. In this review, we examine our current mouse model of HAEC and how it informs our understanding of the disease. We also describe current emerging research that highlights the potential future of HAEC treatment.
Pituitary Apoplexy Following Severe Diabetic Ketoacidosis, With Two Uncommon Complications of Supraventricular Tachycardia and Acute Limb Ischemia in a Patient with Neglected Pituitary Adenoma and Undiagnosed Diabetes Mellitus: A Rare Clinical Association
Authors: Pattankar Sanjeev , Chauhan Phulrenu , Kapadia Farhad , Sankhe Milind 
Published: June 2022

AbstractPituitary apoplexy (PA) is a clinical emergency arising from acute ischemia or hemorrhage of the pituitary gland. A small subset of pituitary adenomas present with an apoplectic crisis, with common symptoms being headache, nausea/vomiting, visual impairment, ophthalmoplegia, altered sensorium, panhypopituitarism, etc. Though diabetic ketoacidosis (DKA) is an established complication of uncontrolled diabetes mellitus, its association with PA is extremely rare. Likewise, supraventricular tachycardia (SVT) and acute limb ischemia (ALI) have rare, reported association with DKA. We present one such case of rare associations seen in our clinical practice. A 20-year-old woman was brought to our emergency room with headache, breathlessness, and altered sensorium. Clinical and biochemical evaluation revealed SVT, DKA, and right lower limb ALI. On enquiry, patient was found to be diagnosed with pituitary adenoma 2 years ago and lost to follow-up. PA was detected on neuroimaging and confirmed histopathologically. Possibility of PA presenting as DKA and its sequelae exists.
The Effect of the Electronic Health Record on Interprofessional Practice: A Systematic Review
Authors: Robertson Samantha T. , Rosbergen Ingrid C.M. , Burton-Jones Andrew , Grimley Rohan S. , Brauer Sandra G. 
Published: June 2022

Abstract
Background Interprofessional practice and teamwork are critical components to patient care in a complex hospital environment. The implementation of electronic health records (EHRs) in the hospital environment has brought major change to clinical practice for clinicians which could impact interprofessional practice.
Objectives The aim of the study is to identify, describe, and evaluate studies on the effect of an EHR or modification/enhancement to an EHR on interprofessional practice in a hospital setting.
Methods Seven databases were searched including PubMed, Scopus, Web of Science, CINAHL, Cochrane, EMBASE, and ACM Digital Library until November 2021. Subject heading and title/abstract searches were undertaken for three search concepts: “interprofessional” and “electronic health records” and “hospital, personnel.” No date limits were applied. The search generated 5,400 publications and after duplicates were removed, 3,255 remained for title/abstract screening. Seventeen studies met the inclusion criteria and were included in this review. Risk of bias was quantified using the Quality Assessment Tool for Studies with Diverse Designs. A narrative synthesis of the findings was completed based on type of intervention and outcome measures which included: communication, coordination, collaboration, and teamwork.
Results The majority of publications were observational studies and of low research quality. Most studies reported on outcomes of communication and coordination, with few studies investigating collaboration or teamwork. Studies investigating the EHR demonstrated mostly negative or no effects on interprofessional practice (23/31 outcomes; 74%) in comparison to studies investigating EHR enhancements which showed more positive results (20/28 outcomes; 71%). Common concepts identified throughout the studies demonstrated mixed results: sharing of information, visibility of information, closed-loop feedback, decision support, and workflow disruption.
Conclusion There were mixed effects of the EHR and EHR enhancements on all outcomes of interprofessional practice, however, EHR enhancements demonstrated more positive effects than the EHR alone. Few EHR studies investigated the effect on teamwork and collaboration.
De Novo Glioblastoma Masqueraded within a Hemispheric Dural Meningiomatosis: Rare Imaging Findings and Rationale for Two-Staged Resection
Authors: Zacharewski Nicholas , Movahed-Ezazi Misha , Song Xianyuan , Mehta Tapan , Manjila Sunil 
Published: June 2022

Abstract
Introduction Collision tumors present as histologically different juxtaposed neoplasms within the same anatomical region, independent of the adjacent cell population. De novo intracranial collision tumors involving metachronous primary brain neoplasms alongside dural meningiomatosis are not well documented in the literature.
Clinical Presentation We present staged surgical management of a 72-year-old female with known left hemispheric stable dural-based convexity mass lesions over 10 years and new-onset expressive aphasia and headaches. MRI had revealed left supratentorial dural-based enhanced masses consistent with en plaque meningiomatosis. Embolization angiography showed an unusual tumor blush from an aberrant branch of anterior cerebral artery suggesting a deeper focal intra-axial nature; a stage 1 craniotomy for dural-based tumor resection was completed with diagnosis of a meningioma (WHO grade 1). Intraoperatively, a distinct intra-axial deep discrete lesion was verified stereotactically, concordant with the location of tumor blush. The patient made a complete neurological recovery from a transient postoperative supplemental motor area syndrome in a week. Subsequent postoperative follow-up showed worsening of right hemiparesis and MRI showed an increase in residual lesion size and perilesional edema, which prompted a stage 2 radical resection of a glioblastoma, WHO grade 4. She improved neurologically after surgery with steroids and physical therapy. At 15 months following adjuvant therapy, she remains neurologically intact throughout the postoperative course, with no recurrent tumor on MRI.
Conclusion A de novo glioblastoma presented as a masquerading lesion within hemispheric convexity meningiomatosis in an elderly patient with no prior radiation/phakomatosis, inciting a non-causal juxtapositional coexistence. The authors highlight rare pathognomonic angiographic findings and the rationale for two-staged resections of these collision lesions that led to excellent clinicoradiological outcome.