bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multidimensional coding architecture of the vagal interoceptive system
Authors: Zhao Qiancheng , Yu Chuyue D. , Wang Rui , Xu Qian J. , Dai Pra Rafael , Zhang Le , Chang Rui B. 
Published: March 2022

AbstractInteroception, the ability to timely and precisely sense changes inside the body, is critical for survival1–4. Vagal sensory neurons (VSNs) form an important body-to-brain connection, navigating visceral organs along the rostral–caudal axis of the body and crossing the surface–lumen axis of organs into appropriate tissue layers5,6. The brain can discriminate numerous body signals through VSNs, but the underlying coding strategy remains poorly understood. Here we show that VSNs code visceral organ, tissue layer and stimulus modality—three key features of an interoceptive signal—in different dimensions. Large-scale single-cell profiling of VSNs from seven major organs in mice using multiplexed projection barcodes reveals a ‘visceral organ’ dimension composed of differentially expressed gene modules that code organs along the body’s rostral–caudal axis. We discover another ‘tissue layer’ dimension with gene modules that code the locations of VSN endings along the surface–lumen axis of organs. Using calcium-imaging-guided spatial transcriptomics, we show that VSNs are organized into functional units to sense similar stimuli across organs and tissue layers; this constitutes a third ‘stimulus modality’ dimension. The three independent feature-coding dimensions together specify many parallel VSN pathways in a combinatorial manner and facilitate the complex projection of VSNs in the brainstem. Our study highlights a multidimensional coding architecture of the mammalian vagal interoceptive system for effective signal communication.
A conserved sequence in the small intracellular loop of tetraspanins forms an M-shaped inter-helix turn
Authors: Reppert Nikolas , Lang Thorsten 
Published: March 2022

AbstractTetraspanins are a family of small proteins with four transmembrane segments (TMSs) playing multiple roles in human physiology. Nevertheless, we know little about the factors determining their structure. In the study at hand, we focus on the small intracellular loop (SIL) between TMS2 and TMS3. There we have identified a conserved five amino acid core region with three charged residues forming an M-shaped backbone, which we call M-motif. The M´s plane runs parallel to the membrane surface and the central amino acid constitutes the inter-helix turning point. At the second position of the M-motif, in tetraspanin crystal structures we identified a glutamate oriented towards a lysine in the juxtamembrane region of TMS1. Using Tspan17 as example, we find that by mutating either the glutamate or juxtamembrane-lysine, but not upon glutamate/lysine swapping, expression level, maturation and ER-exit are reduced. We conclude that the SIL is more than a short linking segment but propose it is involved in shaping the tertiary structure of tetraspanins.
Impact of the COVID-19 pandemic on oral health and psychosocial factors
Authors: Ciardo Antonio , Simon Marlinde M. , Sonnenschein Sarah K. , Büsch Christopher , Kim Ti-Sun 
Published: March 2022

AbstractThe objective of this study was to investigate oral health-related quality of life (OHRQoL) in times of the COVID-19 pandemic and to examine a possible association to psychosocial factors like psychological stress and symptoms of depression and anxiety disorders. Secondary research questions were whether people changed oral hygiene regimens during the COVID-19 pandemic and to what extent dental symptoms existed and developed compared to pre-pandemic. For this cross-sectional study a survey has been conceptualized to determine OHRQoL, stress, depression and anxiety and their specific confounders in a German cohort. Validated questionnaires as OHIP-G14, PHQ-Stress and PHQ-4 have been implemented. Altogether 1178 participants completed the survey between May and August 2020. The overall OHIP-G14 sum score of 4.8 ± 7.5 indicated good OHRQoL. 21% of the participants (n = 248) reported toothache, 23% (n = 270) mucosal problems, 31% (n = 356) hypersensitivity of the teeth and 27% (n = 305) myofacial pain. The PHQ-Stress score (4.5 ± 3.5) demonstrated a mild severity of stress. Depression and anxiety level has been mild to moderate (PHQ-4 score: 2.4 ± 2.6). 38% of the participants stated subjectively greater emotional burden compared to pre-pandemic. Statistically significant differences exist for OHRQoL, stress, anxiety and depression levels between participants with greater, equal or less emotional burden compared to pre-pandemic. COVID-19 history and aggravated levels of depression, anxiety, and stress seem to associate with lower OHRQoL. Psychosocial consequences during pandemic times and their association to oral health should be further investigated.
The prevalence of dental developmental anomalies among childhood cancer survivors according to types of anticancer treatment
Authors: Halperson Elinor , Matalon Vered , Goldstein Gal , Saieg Spilberg Shirly , Herzog Karin , Fux-Noy Avia , Shmueli Aviv , Ram Diana , Moskovitz Moti 
Published: March 2022

AbstractSurvival following childhood cancer has increased considerably. In an observational cross-sectional study, we assessed the prevalence of dental developmental anomalies (DDA) among childhood cancer survivors according to types of anticancer treatment. Permanent teeth were examined clinically and radiographically in 121 adolescents with a history of childhood malignancies, to identify DDA, namely hypomineralization or hypoplasia, microdontia, root changes and hypodontia. DDA were observed in 56/121 individuals (46%), in 309/3388 teeth (9%). Hypomineralization or hypoplasia of enamel appeared in 21 (17%) patients. Altered root development appeared in 26 patients and hypodontia affected 13 (10%). Dental anomalies were observed in 36 (43%) individuals who received chemotherapy and not radiation, in 20 (52%) who received radiotherapy, and in 15 (60%) of those who received head and neck radiotherapy. Among patients who received only chemotherapy, young age (6 years or younger) was associated with a higher number of malformed teeth. In conclusion, antineoplastic treatment that combines chemotherapy and radiotherapy appears to increase the risk of DDA. Radiation to the head and neck area was shown to particularly increase the risk of DDA. No specific chemotherapy agent was found to be associated more than the others with DDA.
Natural antibodies and CRP drive anaphylatoxin production by urate crystals
Authors: Wessig Anne Kathrin , Hoffmeister Leonie , Klingberg Annika , Alberts Anika , Pich Andreas , Brand Korbinian , Witte Torsten , Neumann Konstantin 
Published: March 2022

AbstractIn gout, crystallization of uric acid in the form of monosodium urate (MSU) leads to a painful inflammatory response. MSU crystals induce inflammation by activating the complement system and various immune cell types, and by inducing necrotic cell death. We previously found that the soluble pattern recognition molecule C-reactive protein (CRP) recognizes MSU crystals, while enhancing complement activation. In the absence of CRP, MSU crystals still induced complement activation, suggesting additional CRP-independent mechanisms of complement activation. In the present study, we searched for additional MSU crystal-binding complement activators. We found that all healthy individuals, even unborn children, have MSU crystal-specific immunoglobulin M (IgM) in their blood. This indicates that innate IgM, also known as natural IgM, recognizes these crystals. In serum lacking IgM and CRP, MSU crystals showed negligible complement activation as assessed by the production of the anaphylatoxins C4a, C3a, and C5a (listed in order of production via the classical complement pathway). We show that IgM and CRP both activate the classical complement pathway on MSU crystals. CRP was more efficient at fixating active C1 on the crystals and inducing release of the most inflammatory anaphylatoxin C5a, indicating non-redundant functions of CRP. Notably, while CRP recognizes MSU crystals but not the related calcium pyrophosphate dihydrate (CPPD) crystals, natural IgM bound to both, suggesting common and distinct mechanisms of recognition of individual crystal types by complement activators.
A new feed additive composed of urea and soluble carbohydrate coated with wax for controlled release in ruminal fluid
Authors: Inácio Alexandre Guimarães , Ítavo Camila Celeste Brandão Ferreira , Dias Alexandre Menezes , dos Santos Difante Gelson , de Queiroz Joice Ferreira , de Oliveira Lincoln Carlos Silva , dos Santos Geraldo Tadeu , Ítavo Luís Carlos Vinhas 
Published: March 2022

AbstractUrea is a compound widely used as a feed additive for ruminants; however, when used profusely, it can lead animals to intoxication. Another factor that affects the effectiveness of urea is the lack of synchronization between the nitrogen and the availability of carbohydrates, necessary for better development of the ruminal microbiota. In order to circumvent these problems and improve the efficiency in urea use, the present study developed two new nutritional additives (F16 and F17) with different carbohydrate sources. One of the products developed (F16) used sugarcane molasses as a carbohydrate source, while the other (F17) used cassava starch. In addition to the carbohydrate source, both products contained the same amounts of urea, sulfur, calcium carbonate and were coated with carnauba wax. The supplements developed and two other commercial products based on extruded urea (UE) and polymer-coated urea (UP) were tested for solubility and cumulative gas production. The wax used in the coating process of the developed products (F16 and F17) proved to be efficient in reducing the solubility of the ingredients used. During chemical composition analysis it was verified that both supplements developed contained protein equivalent above 150% of crude protein. The cumulative gas production showed a higher production related to the product F17 (p < 0.05). Through thermogravimetric analysis, it was found the chemical integrity of the ingredients that make up the supplements developed. Therefore, is possible to reduce the solubility of urea using carnauba wax as a coating material. The formula with cassava starch associated with urea (F17) had a better synchronization during the degradation of its ingredients.
Constraining the response of continental-scale groundwater flow to climate change
Authors: Mather Ben , Müller R. Dietmar , O’Neill Craig , Beall Adam , Vervoort R. Willem , Moresi Louis 
Published: March 2022

AbstractNumerical models of groundwater flow play a critical role for water management scenarios under climate extremes. Large-scale models play a key role in determining long range flow pathways from continental interiors to the oceans, yet struggle to simulate the local flow patterns offered by small-scale models. We have developed a highly scalable numerical framework to model continental groundwater flow which capture the intricate flow pathways between deep aquifers and the near-surface. The coupled thermal-hydraulic basin structure is inferred from hydraulic head measurements, recharge estimates from geochemical proxies, and borehole temperature data using a Bayesian framework. We use it to model the deep groundwater flow beneath the Sydney–Gunnedah–Bowen Basin, part of Australia’s largest aquifer system. Coastal aquifers have flow rates of up to 0.3 m/day, and a corresponding groundwater residence time of just 2,000 years. In contrast, our model predicts slow flow rates of 0.005 m/day for inland aquifers, resulting in a groundwater residence time of $$\sim $$
∼
400,000 years. Perturbing the model to account for a drop in borehole water levels since 2000, we find that lengthened inland flow pathways depart significantly from pre-2000 streamlines as groundwater is drawn further from recharge zones in a drying climate. Our results illustrate that progressively increasing water extraction from inland aquifers may permanently alter long-range flow pathways. Our open-source modelling approach can be extended to any basin and may help inform policies on the sustainable management of groundwater.
Control of crystallization and magnetic properties of CoFeB by boron concentration
Authors: Kim Jun-Su , Kim Gukcheon , Jung Jinwon , Jung Kuyoul , Cho Jaehun , Kim Woo-Yeong , You Chun-Yeol 
Published: March 2022

AbstractControlling the crystallinity of CoFeB is the most essential issue for designing various spintronics devices. Here we show the microstructure and magnetic properties of MgO/CoFeB/MgO structures for various boron concentration. We present the effect of boron on the crystallinity of CoFeB into two categories: the critical boron concentration (5 ~ 6%) at which CoFeB crystallizes and the effect of remaining boron (0 ~ 5%) in the crystallized CoFeB. And the trends of the saturation magnetization, exchange stiffness, exchange length, domain wall energy and Gilbert damping constant according to the boron concentration are provided. Abrupt variation of properties near the critical boron concentration (5 ~ 6%) and a noticeable change in the crystallized CoFeB (0 ~ 5%) are confirmed, revealing a clear causal relationship with the structural analysis. These results propose that the crystallization, microstructure, and major magnetic properties of CoFeB are governed by the amount of boron, and emphasize the need for delicate control of boron concentration.
Quantal response equilibrium for the Prisoner’s Dilemma game in Markov strategies
Authors: Kozitsina T. S. , Kozitsin I. V. , Menshikov I. S. 
Published: March 2022

AbstractWithin the studies of human cooperation, there are gaps that require further investigation. One possible area for growth is developing theoretical concepts which describe high levels of cooperation. In this paper, we present a symmetrical quantal response equilibrium (QRE) in Prisoner’s Dilemma game (PD) constructed in Markov strategies (tolerance to defection and mutual cooperation). To prove the adequacy of the resulting equilibrium, we compare it with the previously found Nash equilibrium in PD in Markov strategies: the QRE converges with the Nash equilibrium that corresponds with the theory. Next, we investigate the properties of QRE in PD in Markov strategies by testing it against experimental data. For low levels of rationality, the found equilibrium manages to describe high cooperation. We derive the levels of rationality under which the intersection between Nash and QRE occurs. Lastly, our experimental data suggest that QRE serves as a dividing line between behavior with low and high cooperation.
An ultrafast time-resolution method based on picosecond pulsed laser for determining rock fracture toughness at multipoint during the crack propagation
Authors: Zhang Mingyuan , Li Dejian , Yang Liu , Chen Lu , Shen Muao , Huo Junhao , Li Yingjun 
Published: March 2022

AbstractAn innovative ultrafast time-resolution method based on a picosecond pulsed laser was employed to investigate the mode-I crack propagation characteristics of fractured rock. Its time resolution is as fast as the degree of 45 picoseconds. Then, a series of three-point compressive loading tests with this method were conducted on tuff semi-circular bend (SCB) specimens. Based on this method, we found that the mode-I fracture process of the tuff specimens were composed of repeated crack initiation, arrest, and re-initiation. In addition, the experimental results showed that the fracture rates of the tuff specimens in the initial 10 μs were 636 m/s, 663.9 m/s, and 578 m/s. In comparison, the fracture rates of the specimens were 11.19 m/s, 19.23 m/s, 26.79 m/s during the whole fracture process. As a typical heterogeneous material with primary defects, rock has different fracture toughness at different locations. Therefore, we proposed a new method for determining rock fracture toughness at multipoint during the crack propagation. This new method emphasizes the effect of fracture toughness on crack propagation, which enables to determine the fracture toughness at multipoint and is closer to the original definition of fracture toughness.
Increased O-GlcNAcylation promotes IGF-1 receptor/PhosphatidyI Inositol-3 kinase/Akt pathway in cervical cancer cells
Authors: Jiménez-Castillo Victoria , Illescas-Barbosa Daniela , Zenteno Edgar , Ávila-Curiel Beatriz Xóchitl , Castañeda-Patlán Maria Cristina , Robles-Flores Martha , De Oca Daniel Montante-Montes , Pérez-Campos Eduardo , Torres-Rivera Anayetzin , Bouaboud Abdelouhab , Pagesy Patrick , Solórzano-Mata Carlos Josué , Issad Tarik 
Published: March 2022

AbstractO-linked β-N-acetylglucosaminylation (O-GlcNAcylation) is a reversible post-translational modification on serine and threonine residues of cytosolic, nuclear and mitochondrial proteins. O-GlcNAcylation level is regulated by OGT (O-GlcNAc transferase), which adds GlcNAc on proteins, and OGA (O-GlcNAcase), which removes it. Abnormal level of proteinO-GlcNAcylation has been observed in numerous cancer cell types, including cervical cancer cells. In the present study, we have evaluated the effect of increasing proteinO-GlcNAcylation on cervical cancer-derived CaSki cells. We observed that pharmacological enhancement of proteinO-GlcNAcylation by Thiamet G (an inhibitor of OGA) and glucosamine (which provides UDP-GlcNAc substrate to OGT) increases CaSki cells proliferation, migration and survival. Moreover, we showed that increasedO-GlcNAcylation promotes IGF-1 receptor (IGF1R) autophosphorylation, possibly through inhibition of protein tyrosine-phosphatase 1B activity. This was associated with increased IGF-1-induced phosphatidyl-Inositol 3-phosphate production at the plasma membrane and increased Akt activation in CaSki cells. Finally, we showed that proteinO-GlcNAcylation and Akt phosphorylation levels were higher in human cervical cancer samples compared to healthy cervix tissues, and a highly positive correlation was observed betweenO-GlcNAcylation level and Akt phosphorylation in theses tissues. Together, our results indicate that increasedO-GlcNAcylation, by activating IGF1R/ Phosphatidyl inositol 3-Kinase (PI-3K)/Akt signaling, may participate in cervical cancer cell growth and proliferation.
Evaluation of the impact of pre-operative stereotactic radiotherapy on the acute changes in histopathologic and immune marker profiles of brain metastases
Authors: Kotecha Rupesh , Tonse Raees , Menendez Miguel A. Ramirez , Williams Andre , Diaz Zuanel , Tom Martin C. , Hall Matthew D. , Mehta Minesh P. , Alvarez Reinier , Siomin Vitaly , Odia Yazmin , Ahluwalia Manmeet S. , McDermott Michael W. 
Published: March 2022

AbstractThe unique acute effects of the large fractional doses that characterize stereotactic radiosurgery (SRS) or radiotherapy (SRT), specifically in terms of antitumor immune cellular processes, vascular damage, tumor necrosis, and apoptosis on brain metastasis have yet to be empirically demonstrated. The objective of this study is to provide the first in-human evaluation of the acute biological effects of SRS/SRT in resected brain metastasis. Tumor samples from patients who underwent dose-escalated preoperative SRT followed by resection with available non-irradiated primary tumor tissues were retrieved from our institutional biorepository. All primary tumors and irradiated metastases were evaluated for the following parameters: tumor necrosis, T-cells, natural killer cells, vessel density, vascular endothelial growth factor, and apoptotic factors. Twenty-two patients with irradiated and resected brain metastases and paired non-irradiated primary tumor samples met inclusion criteria. Patients underwent a median preoperative SRT dose of 18 Gy (Range: 15–20 Gy) in 1 fraction, with 3 patients receiving 27–30 Gy in 3–5 fractions, followed by resection within median interval of 67.8 h (R: 18.25–160.61 h). The rate of necrosis was significantly higher in irradiated brain metastases than non-irradiated primary tumors (p < 0.001). Decreases in all immunomodulatory cell populations were found in irradiated metastases compared to primary tumors: CD3 + (p = 0.003), CD4 + (p = 0.01), and CD8 + (p = 0.01). Pre-operative SRT is associated with acute effects such as increased tumor necrosis and differences in expression of immunomodulatory factors, an effect that does not appear to be time dependent, within the limited intervals explored within the context of this analysis.
Resting-state theta activity is linked to information content-specific coding levels during response inhibition
Authors: Pscherer Charlotte , Mückschel Moritz , Bluschke Annet , Beste Christian 
Published: March 2022

AbstractThe neurophysiological processes underlying the inhibition of impulsive responses have been studied extensively. While also the role of theta oscillations during response inhibition is well examined, the relevance of resting-state theta activity for inhibitory control processes is largely unknown. We test the hypothesis that there are specific relationships between resting-state theta activity and sensory/motor coding levels during response inhibition using EEG methods. We show that resting theta activity is specifically linked to the stimulus-related fraction of neurophysiological activity in specific time windows during motor inhibition. In contrast, concomitantly coded processes related to decision-making or response selection as well as the behavioral inhibition performance were not associated with resting theta activity. Even at the peak of task-related theta power, where task-related theta activity and resting theta activity differed the most, there was still predominantly a significant correlation between both types of theta activity. This suggests that aspects similar to resting dynamics are evident in the proportion of inhibition-related neurophysiological activity that reflects an “alarm” signal, whose function is to process and indicate the need for cognitive control. Thus, specific aspects of task-related theta power may build upon resting theta activity when cognitive control is necessary.
Drug and pesticide usage for sea lice treatment in salmon aquaculture sites in a Canadian province from 2016 to 2019
Authors: Hamoutene Dounia , Oldford Vanessa , Donnet Sebastien 
Published: March 2022

AbstractWe used 4 years of publicly available data (2016–2019) on chemical usage at salmon sites with information on production, stocking, locations and environmental conditions to explore patterns of anti-sea lice treatments in a Canadian province. Results show that sequential chemical treatments are prevalent, emamectin benzoate (EMB) with azamethiphos being the most used combination with a decrease in ivermectin usage. Relatively high rates of usage of EMB per fish biomass may point to potential lice resistance patterns with information needed on mechanisms and local populations. Lower or no chemical usage at some sites indicate less sea lice infestations likely influenced by localized site conditions (coves), and a lessened need for medication due to the usage of cleaner fish and possibly other non-chemical methods (not documented in this report). The year/climate influenced chemical input only in sites with higher treatment levels likely due to effects on sea lice growth and reproduction. Observed differences between years are warmer surface temperature in the fall, a higher freshwater input in spring, and stronger wind conditions for 2017 and 2018 with more medication usage for these two years. The lack of significant effect of site distances calculated in zones of influence based on 24 h potential connectivity patterns highlight the need to refine the resolution of hydrodynamic processes.