bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure and properties of antimicrobial peptides produced by antagonist microorganisms isolated from Siberian natural objects
Authors: Babich Olga , Milentyeva Irina , Dyshlyuk Lyubov , Ostapova Elena , Altshuler Olga 
Published: January 2022

Introduction. Public healthcare urgently needs new pharmaceuticals – alternative to traditional antibiotics – that pathogens develop no resistance to. Of special interest in this regard are antimicrobial, ribosomally synthesized bacterial peptides or bacteriocins. In this work, we aimed to study the structure and properties of antimicrobial peptides produced by antagonist microorganisms isolated from the natural objects of the Siberian region.
Study objects and methods. The study objects were bacteria isolated from the natural sources of Kuzbass. After culturing bacteria, total protein was precipitated from the culture fluid and separated into fractions by gel permeation HPLC. Their amino acid sequences were determined by MALDI-TOF mass spectrometry. The antibacterial (against Bacillus pumilus and Escherichia coli) and fungicidal (against Aspergillus flavus and Aspergillus niger) properties of the peptides were studied by the disk diffusion method.
Results and discussion. Seven peptides with different amino acid sequences were isolated from the culture fluid of bacteria, five of which had no analogues in the PepBank and Uniprot data banks. The peptide with an amino acid sequence of VMCLARKCSQGLIVKAPLM (2061.66 Da) was homologous to the cysteine membrane protein Giardia lamblia P15, and the peptide with an amino acid sequence of AVPSMKLCIQWSPVRASPCVMLGI (2587.21 Da) showed a homology with the Planctomycetes bacterium I41 peptides. We found antibacterial (against gram-positive and gram-negative bacteria) and fungicidal (against Aspergillus) properties in the peptide fractions.
Conclusion. Antimicrobial peptides produced by bacteria isolated from the natural objects of the Siberian region can be used to create pharmaceuticals as an alternative to traditional antibiotics to treat infectious diseases.
Improving the nutritional properties of Brassica L. vegetables by spontaneous fermentation
Authors: Parada Romina B. , Marguet Emilio , Campos Carmen A. , Vallejo Marisol 
Published: January 2022

Introduction. Brassica L. vegetables are rich in fiber, minerals, and bioactive compounds. Lactic fermentation can improve their nutritional value. The goal of this study was to evaluate phytase, calcium, phytic acid, total phenolic content, and antioxidants during spontaneous fermentation of white cabbage, red cabbage, and Chinese cabbage.
Study objects and methods. The research featured samples of water extract, methanol extract, and brine. The procedure involved monitoring lactic bacteria and pH during cabbage fermentation. Diphenyl-1-picrylhydrazyl radical (DPPH) scavenging assay and cupric reducing antioxidant capacity (CUPRAC) assay were used to measure the antioxidant activity and Folin-Ciocalteau method to determine total phenolic content in the water and methanol extracts. In the brine samples, we studied calcium, phytic acid, and phytase activity.
Results and discussion. The samples of white and red cabbage displayed the highest phytase activity on days 5–10 and had a maximal decrease of phytic acid and increase of calcium concentration, while in Chinese cabbage these processes occurred gradually throughout the fermentation. The total phenolic content in the brine and extracts was very similar for all the cultivars throughout the fermentation process. A continuous release from the solid phase to brine could be observed during the first ten days of fermentation. DPPH and CUPRAC assays revealed a similar phenomenon for the total phenolic content. The antioxidant capacity decreased in the water and methanol extracts and increased in the brine. At the end of fermentation, the red cabbage samples demonstrated a significant increase in the total phenolic content and total antioxidant activity, which was less prominent in the Chinese cabbage. The samples of white cabbage, on the contrary, showed a decrease in these parameters.
Conclusion. Fermentation made it possible to increase the concentration of free calcium in white, red, and Chinese cabbages, as well as improve the antioxidant capacity of red and Chinese cabbages.
Daily Level Association of Physical Activity and Performance on Ecological Momentary Cognitive Tests in Free-living Environments: A Mobile Health Observational Study
Authors: Zlatar Zvinka Z , Campbell Laura M , Tang Bin , Gabin Spenser , Heaton Anne , Higgins Michael , Swendsen Joel , Moore David J , Moore Raeanne C 
Published: January 2022

Background
Research suggests that physical activity (PA) has both acute and chronic beneficial effects on cognitive function in laboratory settings and under supervised conditions. Mobile health technologies make it possible to reliably measure PA and cognition in free-living environments, thus increasing generalizability and reach. Research is needed to determine whether the benefits of PA on cognitive function extend from the laboratory to real-world contexts.

Objective
This observational study aims to examine the association between daily fluctuations in PA and cognitive performance using mobile health technologies in free-living environments.

Methods
A total of 90 adults (mean age 59, SD 6.3 years; 65/90, 72% men) with various comorbidities (eg, cardiovascular risk and HIV) and different levels of baseline cognition (ranging from cognitively normal to impaired) completed ecological momentary cognitive tests (EMCTs) on a smartphone twice daily while wearing an accelerometer to capture PA levels for 14 days. Linear mixed-effects models examined the daily associations of PA with executive function and verbal learning EMCTs. Moderation analyses investigated whether the relationship between daily PA and daily performance on EMCTs changed as a function of baseline cognition, cardiovascular risk, and functional status (independent vs dependent).

Results
Days with greater PA were associated with better (faster) performance on an executive function EMCT after covariate adjustment (estimate −0.013; β=−.16; P=.04). Moderation analyses (estimate 0.048; β=.58; P=.001) indicated that days with greater PA were associated with better (faster) executive function performance in individuals who were functionally dependent (effect size −0.53; P<.001) and not in functionally independent adults (effect size −0.01; P=.91).

Conclusions
EMCTs may be a sensitive tool for capturing daily-level PA-related fluctuations in cognitive performance in real-world contexts and could be a promising candidate for tracking cognitive performance in digital health interventions aimed at increasing PA. Further research is needed to determine individual characteristics that may moderate the association between daily PA and EMCT performance in free-living environments.
Molecular insights into the structural and dynamical changes of calcium channel TRPV6 induced by its interaction with phosphatidylinositol 4,5-bisphosphate
Authors: Wang Lingyun , Cai Ruiqi , Chen Xing-Zhen , Peng Ji-Bin 
Published: January 2022

Transient receptor potential vanilloid subfamily member 6 (TRPV6) is an
epithelial Ca channel that regulates the initial
step of the transcellular Ca transport pathway.
TRPV6 is expressed in the kidney, intestine, placenta, and other
tissues, and the dysregulation of the channel is implicated in several
human cancers. It has been reported that phosphatidylinositol
4,5-bisphosphate (PIP ) activates TRPV6 and its close
homologue TRPV5; however, the underlying molecular mechanism is less
clear. Recently, a structure of rabbit TRPV5 in complex with dioctanoyl
(diC ) PIP , a soluble form of PIP
, was determined by cryo-electron microscopy. Based on
this structure, the structural model of human TRPV6 with PIP
was set up, and then molecular dynamics simulations
were performed for TRPV6 with and without PIP .
Simulation results show that the positively charged residues responsible
for TRPV5 binding of diC PIP are
involved in the interactions between TRPV6 and PIP .
The binding of PIP to TRPV6 increases the distance
between the diagonally opposed residues D542 in the selectivity filter
and that between the diagonally opposed M578 residues in the lower gate
of TRPV6. A secondary structural analysis reveals that residues of M578
in TRPV6 undergo structural and position changes during binding of PIP
with TRPV6. In addition, principal component analysis
indicates that the binding of PIP increases the
dynamical motions of both the selectivity filter and the lower gate of
TRPV6. These changes induced by PIP favor the channel
opening. Thus, this study provides a basis for understanding the
mechanism underlying the PIP -induced TRPV6 channel
activation.
Super-elasticity in vitro assessment of CuNiTi wires according to their Austenite finish temperature and the imposed displacement
Authors: Copelovici Noémie , Tran Maï-Linh , Lefebvre François , Laheurte Pascal , Wagner Delphine 
Published: January 2022

ABSTRACT

Objectives
To assess the super-elasticity of CuNiTi wires (Ormco, Glendora, Calif) according to their Austenite finish temperature (Af) and to the imposed displacement. The secondary objective was to compare the wire dimensions with the stated measurements and to study interbatch variability.

Materials and Methods
10 types of CuNiTi wires (Ormco, Glendora, Calif) (n = 350) were investigated at 36 ± 1°C, with conventional brackets (Victory Series, 3M Unitek, Monrovia, Calif). Tensile test with coronoapical displacement ranging from 1 to 5 mm of the canine bracket was imposed. The wire dimensions were initially measured from two batches (n = 10).

Results
Dimensional heterogeneity varied by ± 2.00% compared to the manufacturer's data, and even up to 5.54% for 0.014-inch CuNiTi (P = .00069). However, all unloading forces were reproducible. In decreasing order, the forces delivered by a CuNiTi 27 were greater than those with CuNiTi 35 and 40. The super-elasticity was expressed only for displacements of 1 to 2 mm, at best up to 3 mm for 0.014-inch CuNiTi 27.

Conclusions
The value of Af as well as the amount of imposed displacement seem to influence the expression of the super-elasticity of CuNiTi wires and the amount of corrected malocclusion. Among the tested wires, under these experimental conditions, 0.014-inch wire could be suitable as a first archwire. CuNiTi 35, therefore, seems to offer the best compromise among the force level, the expression of super-elasticity and the amount of malocclusion correction.
PERSONAL RESOURCES OF OVERCOMING A PROFESSIONAL BURNOUT BY MEDICAL WORKERS DURING THE COVID-19 PANDEMIC
Authors: Рерке Виктория Игоревна , Демаков Владимир Иванович , Бубнова Ирина Сергеевна 
Published: January 2022

Современная эпидемически нестабильная обстановка снижает чувство безопасности и защищенности каждого человека, а ситуация угрозы жизни становится все более привычной в мирной жизни. В этот социальный период увеличивается риск эмоционального истощения и снижения профессиональной активности медицинских работников. Значимость решения данной проблемы связана еще и с тем, что синдром эмоционального выгорания имеет сложную форму и трактуется неоднородно с точки зрения первопричин и конечного исхода.Статья посвящена изучению личностных ресурсов преодоления профессионального выгорания у медицинских работников в период пандемии COVID-19. Выявлены фазы и особенности профессионального выгорания у медицинских работников в период пандемии. Установлено, что умеренно выраженные показатели жизнестойкости могут выступать личностным ресурсом и фактором преодоления профессионального выгорания. Доказано, что врачи, обладающие жизнестойкостью как одним из личностных ресурсов, отличаются качествами, которые помогают адаптироваться и выстоять в сложных социальных ситуациях.
During the period of an epidemiologically unstable situation in the world, the risk of emotional exhaustion and a decrease in the professional activity among medical workers increases. The relevance and social significance of solving this problem is due to the fact that the burnout syndrome has a complex form and is interpreted heterogeneously from the point of view of root causes and final outcome. In this turn, it is necessary to find out some psychological means and methods. The purpose of the article is to explore a personal resource of overcoming a professional burnout by medical workers during the COVID-19 pandemic. It has been proven that doctors who possess resilience as one of the personal resources are distinguished by personal qualities that can help to adapt and withstand difficult social situations. The personal resources to overcome the professional burnout by health workers are characterized such categories as dominance, expressiveness, high standard behavior, courage, practicality, diplomacy, nonconformism, high self-control and tension. The health workers sown in this sample are autonomous, independent, firm and resilient. Intransigence, independence and a certain amount of stubbornness can be personal resources to overcome professional burnout, as it reflects the active and independent position by employees. The features of professional burnout by medical workers during a pandemic have been identified. It is found, that a high level of resilience in the structure of personal resources is a factor in overcoming professional burnout among doctors.
Iäkkäiden etäjumppa kunnissa koronapandemian aikaan
Authors: Iltanen Sonja , Topo Päivi 
Published: January 2022

Etäjumppa tarjoaa kunnille uudenlaisen tavan järjestää iäkkäiden terveysliikuntaa ja iäkkäille ihmisille mahdollisuuden osallistua siihen. Artikkelimme tavoitteena on luoda kokonaiskuva siitä, miten koronapandemia vaikutti iäkkäiden etäjumpan toteutukseen eli implementaatioon. Tutkimuksessa seurataan vuosina 2019–2022 seitsemää kuntaa, jotka toteuttivat pilotteja aiemmassa kehittämishankkeessa. Aineisto kerättiin elisitaatiohaastatteluilla. Haastateltavina videopuheluissa oli 12 etäjumppaa toteuttanutta ammattilaista. Aineistosta etsittiin etäjumpan toteutukseen vaikuttavia tekijöitä koronapandemian aikaan. Implementaatiota tarkasteltiin hyväksyttävyyden, käyttöönoton, soveltuvuuden, käytettävyyden, pysyvyyden, kustannusten, leviämisen ja juurtumisen näkökulmista. Tulostemme mukaan koronan tuomat rajoitukset muuttivat organisaatioiden, etäjumpan toteuttajien ja etäjumppaajien toimintaa. Koronapandemia aiheutti merkittävän riskin iäkkäiden etäjumpan jatkumiselle, mutta toisaalta se vahvisti motivaatiota, osaamista ja laitekantaa, jotka edesauttavat etäjumpan järjestämistä jatkossa. Kriisi- tai muutostilanteessa toiminnan jatkuvuuteen näyttää olennaisesti vaikuttavan se, miten vahvasti toiminta on ehtinyt vakiintua osaksi kunnan toimintaa. Implementaatio on moniulotteinen ja jatkuvasti muuttuva prosessi, ja on tärkeää tutkia, miten sitä voidaan tukea.
Online exercise for older adults in municipalities during the COVID-19 pandemic
Online exercise is a novel method for organizing health exercise for older adults, and for them to participate. Our aim is to provide a comprehensive view of how the COVID-19 pandemic affected the implementation of online exercise for older adults. This is a follow-up study (2019–2022) of seven municipalities that piloted online exercise for older adults in a previous development study. The data were gathered by videoconferencing applying elicitation interviews with professionals executing local online exercise (n=12). Factors affecting the implementation of online exercise during the COVID-19 pandemic were examined from perspectives of politics and policies, actions of organizations, executors and online exercisers, relation of technology and abilities, cultural views, and evaluation and knowledge. The outcomes of implementation were analyzed from the viewpoints of acceptability, adoption, appropriateness, feasibility, fidelity, cost, coverage, and sustainability. Our results suggest that the restriction due to the pandemic created a significant risk for the sustainability of online exercise, but also strengthened the motivation, skills and technological facilities – factors that support organizing online exercise in the future. Implementation is a multidimensional and constantly changing process and studying it from the viewpoints applied in this study may improve the comprehension of the process. It is necessary to identify ways of supporting successful implementation.
Stuttering frequency on content and function words in pre-school and school-age Jordanian Arabic-speaking children who stutter
Authors: Alqhazo Mazin , Alfwaress Firas 
Published: January 2022

Abstract
This study investigated the influence of loci of content and function words on stuttering frequency in the speech of Arabic children who stutter. Participants were 85 children who stutter (24 preschool, 61 school age). The preschool children who stutter were 17 males and 7 females with a mean age of 4.58 ± 0.50 (range: 4-5 years old). The school age children who stutter were 56 males and 5 females with a mean age of 10.64 ± 2.76 (range: 6-16 years old). No significant difference was found between the preschool and school age children who stutter in the mean percentage of stuttering on both content and function words. For school age children who stutter, results showed a significantly higher percentage of stuttering on function words compared to content words in the mild level of stuttering (p = .010). Taking severity as a continuous variable, results indicated a significant positive correlation between scores on the Stuttering Severity Instrument-4 (SSI-4) and loci of stuttering on both content and function words. The results also revealed a significant negative correlation between age (as a continuous variable) and loci of stuttering in the category of function words. The findings of the current study provide new information about the impact of word type (function vs. content words) on stuttering in Arabic-speaking children.
STUDY ALIRAN DAYA (LOAD FLOW) PADA SISTEM DISTRIBUSI TENAGA LISTRIK GEDUNG PASCA SARJANA UNIVERSITAS BHAYANGKARA SURABAYA
Authors: Kiswantono Agus , Ilmi Firmansyah Darul 
Published: January 2022

Studi aliran beban dimaksudkan untuk memperoleh informasi mengenai alirandaya atau tegangan pada suatu jaringan sistem tenaga listrik. Informasi ini sangat dibutuhkan untuk mengevaluasi unjuk kerja sistem tenaga. Permasalahan aliran daya mencakup perhitungan aliran dan tegangan sistem pada terminal atau bus tertentu. penelitian evaluatif dengan menggunakan metode penelitian operation research/action research, Manfaat studi aliran daya ini yaitu, untuk mengetahui tegangan pada setiap simpul yang ada pada sistem, untuk mengetahui semua peralatan apakah memenuhi batas – batas yang ditentukan untuk meyalurkan daya yang diinginkan, dan untuk memperoleh kondisi mula pada perencanaan sistem yang baru. Studi study aliran beban ini mengambil contoh pada implementasi sistem tenaga listrik di lembaga pendidikan Universitas Bhayangkara Surabaya, dengan karakteristik beban terpusat (lumped load), jaringan sistem distribusi tenaga listrik berupa jaringan radial dan sumber tegangan berasal dari saluran menengah PLN 20 kV menuju ke titik – titik beban. Dari hasil perhitungan aliran daya berbantuan program ETAP (Electrical Transient Analyzer Program) dapat disimpulkan bahwa sistem jaringan listrik sudah baik.Hasil yang diperoleh adalah Circuit breaker pada CB4 mengalami overload dan pada bus 24, bus 4 dan bus 6 mengalami undervoltage. Sedangkan aliran daya masih memenuhi standar menurut hasil Text Report pada ETAP.
The naive picture and unbridled world in the story Lilika by Dragoslav Mihailović
Authors: Hamović Valentina 
Published: January 2022

This paper deals with the motifs of eroticism in the story Lilika by Dragoslav Mihailović, in the context of the growing up and maturing of the young ten-year-old heroine. The erotic in this story is brought down to the level of pornography – animal instincts in the scenes of the girl’s drunken mother and stepfather grabbing each other by the arms and legs in front of the child, slipping hands under the skirt, chasing each other with knives and pushing their tongues into each other’s mouth. They stretch the sphere of eroticism to extremes, which is, in this case, thanatically tainted. And such a bestial life that is led by the stepfather and the mother is not far from true violence over the ten-year-old girl, who is beaten and tied to a bed by the parents; and apart from the physical abuse, whose effects are frequent blood spots and bruises under Lilika’s eyes and uncontrolled night urination, there is an additional passive kindof violence towards the girl – ignoring and neglecting her. In a series of circumstances in which she finds herself unwillingly, there forms an inner framework of the heroine’s unhappy childhood, whose basic problem was posed already at her very birth, and it is the question of her identity.
Immunocytochemistry of effusion fluids: Introduction to SCIP approach
Authors: Shidham Vinod B. , Layfield Lester J. 
Published: January 2022

Due to the remarkably wide morphologic spectrum of reactive mesothelial cells, some of the effusion fluids may be difficult to interpret with objective certainty by cytomorphology alone. Cytomorphology of well to moderately differentiated adenocarcinomas (responsible for the bulk of malignant effusions) may overlap with floridly reactive mesothelial cells. Even mesotheliomas including diffuse malignant epithelioid mesothelioma, are usually cytomorphologically bland without unequivocal features of malignancy.
The intensity of challenge depends on the interpreter’s training or experience level, institutional demographics of patients (such as type of prevalent diseases, predominant sex and age group), technical support, and quality of cytopreparatory processing. In general immunocytochemistry is valuable adjunct to facilitate objective interpretation with or without other ancillary techniques as indicated. An increasing number of immunomarkers is further refining the contribution of immunohistochemistry to this field.
However, application of immunohistochemistry to effusion fluids is relatively challenging because of many variables. Multiple factors such as delay after specimen collection, specimen processing related factors including fixation and storage; ambient conditions under which paraffin blocks are archived (for retrospective testing); antigen retrieval method; duration of antigen retrieval step; antibody clone and dilution; and antibody application time are identical to application of immunohistochemistry in other areas. The significant challenge related to the potential compromization of the immunoreactivity pattern due to exposure to non-formalin fixatives / reagents is also applicable to effusion fluid specimens.
The immunoreactivity results would be compared and corelated with cumulative metadata based on the reported studies performed and validated on formalin-fixed paraffin-embedded tissue sections. Deviating from such protocols may lead to suboptimal results, which is not uncommon in clinical practice with potential compromization of patient care and related liability. Because of this, it is critical to perform immunocytochemistry on formalin-fixed cell-block sections only.
In addition, unless the interpretation criteria for immunohistochemical evaluation of effusion fluids are not modified specifically, it may not be productive in resolving some challenging cases. However, this aspect is not well elaborated in the literature. A basic and critical challenge is finding and locating the cells of interest in cell-block sections of effusion fluids. A unique approach is to choose a fundamental immunopanel which highlight the mesothelial and inflammatory cells in reactive effusion fluids to create the basic map. This allows detection of a ‘second-foreign’ population which can be immunocharacterized further with the help of subtractive coordinate immunoreactivity pattern (SCIP) approach elaborated here.