bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desain Motif sebagai Brand Strategy Pengembangan Galeri Sulam Pinjaik Patah Kabupaten Agam Sumatra Barat
Authors: Masdupi Erni , Kurniawati Tri , Fitra Halkadri , Faisal Dini 
Published: November 2022

Abstrak Pinjaik patah atau jarum jahit yang dipatahkan merupakan brand yang cukup dikenal untuk persoalan produk sulaman terawang di Sumatra Barat, khususnya di kabupaten Agam, Nagari Panampuang. Sayangnya, pengembangan motif yang dilakukan masih minim sehingga diversifikasi produk, pembeda produk hasil sulaman pinjaik patah dan hasil produk kompetitor tidak dapat dibedakan. Perlu dirancang sebuah desain motif baru yang berangkat dari picture mark logo galeri sulam pinjaik patah dengan tujuan untuk menciptakan diversifikasi produk dan brand awareness sulaman pinjaik patah. Metode yang digunakan adalah research and development dengan tahapan 1) Potensi dan Masalah; 2) Pengumpulan informasi; 3) Pengembangan model; 4) Validasi model; 5) Uji coba model. Hasil yang didapatkan yaitu perajin sulaman dapat mengaplikasikan desain motif dalam jenis sulaman terawang dan sulaman menggunakan bordir, hanya saja masih diperlukan revisi dan latihan dari perajin sulaman agar dapat menghasilkan motif yang harmonis. Pengembangan desain dan produk sebaiknya difokuskan pada sulaman terawang yang menjadi unggulan sulam pinjaik patah. Kata kunci: brand awareness, desain motif, pinjaik patah, sulaman AbstractPinjaik patah or a broken sewing needle is a well-known brand for embroidery products in West Sumatra, especially in Agam district, Nagari Panampuang. Unfortunately, the development of the motifs is still minimal so that product diversification, the difference between the product of pinjaik patah embroidery and the product of competitors cannot be distinguished. It is necessary to design a new motif design that departs from the picture mark of the Pinjaik Patah embroidery gallery logo with the aim of creating product diversification and brand awareness of Pinjaik Patah embroidery. The method used is research and development which includes five steps like 1) potentials and problems; 2) gathering information; 3) designing; 4) validation and; 5) design testing. The results obtained are that the embroiderer can apply the motif design in the type of overlay embroidery and embroidery using embroidery, it's just that revision and practice from the embroiderer is still needed in order to produce a harmonious motif. Design and product development should be focused on terawang embroidery which is the flagship of pinjaik patah embroidery. Keywords: brand awareness, embroidery, motif design, pinjaik patah
Early prevention of radial artery occlusion via distal transradial access for primary percutaneous coronary intervention
Authors: Wang Yujie , Liu Zijing , Wu Yongxia , Li Zixuan , Wang Yuntao , Wang Senhu , Xu Rong , Zhang Libin , Wang Yuping , Guo Jincheng 
Published: November 2022

BackgroundConventional transradial access (TRA) has been the preferred access for coronary intervention. Recently, distal radial access (DRA) is introduced as an alternative choice to reduce radial artery occlusion (RAO) risk. The study sought to assess the impact of DRA on early RAO using Doppler ultrasound in patients with ST-segment elevation myocardial infarction (STEMI) who underwent primary percutaneous coronary intervention (PCI).MethodsThis is a prospective, single-center, open-label randomized clinical trial in which patients with indications for primary PCI from January 2022 to September 2022 were assigned to DRA or TRA group with 100 cases in each group. The primary endpoint was the incidence of forearm RAO, evaluated by Doppler ultrasound before discharge.ResultsThe rate of access success was comparable between the DRA and TRA groups (98.0 vs. 94.0%,P= 0.279). Compared with the TRA group, longer puncture time was observed in the DRA group [2.4 (1.7–4.2) min vs. 1.7 (1.4–2.3) min;P&lt; 0.001] whereas the door-to-wire time was not delayed in primary PCI [71 (54–88) min vs. 64 (56–82) min,P= 0.103]. Shorter hemostasis time was required in the DRA group [3.1 (2.7–3.3) h vs. 6.2 (5.9–6.4) h;P&lt; 0.001]. Significant reduction of the incidence of forearm RAO was observed in the DRA group (2.0 vs. 9.0%,P= 0.030). Local hematomas ≤ 5 cm was similar in both groups (4.0 vs. 6.0%,P= 0.516), while those &gt; 5 cm were significantly more frequent in the TRA group (0 vs. 6.0%,P= 0.029).ConclusionDistal radial access is associated with a comparable lower incidence of forearm RAO, shorter hemostasis time, and lower rate of vascular complications compared to TRA in primary PCI.Systematic review registration[https://www.chictr.org.cn], identifier [ChiCTR2200061841].
Construction and validation of a predictive model for hypocalcemia after parathyroidectomy in patients with secondary hyperparathyroidism
Authors: Cheng Jingning , Lv Yong , Zhang Ling , Liu Yafeng 
Published: November 2022

ObjectiveWe aimed to construct and validate a predictive model for the risk of hypocalcemia following parathyroidectomy (PTX) for the treatment of secondary(renal) hyperparathyroidism (SHPT).MethodsInformation regarding patients with SHPT who underwent PTX between January 2019 and April 2022 was collected retrospectively. Univariate and multivariate logistic regression analyses were used to identify independent risk factors for hypocalcemia following PTX and to construct predictive models. The areas under the receiver operating characteristic curve (AUC), the calibration curve, and the clinical decision curve (decision curve analysis, DCA) were used to assess the discrimination, calibration, and level of clinical benefit obtained using the predictive models.ResultsWe studied 238 patients who were randomly allocated in a 7:3 ratio to a training group (n=166) and a test group (n=72). Univariate and multivariate logistic regression analyses were performed, in which three variables (the circulating parathyroid hormone (PTH) and Ca concentrations, and alkaline phosphatase (ALP) activity) were interrogated for possible roles as independent risk factors for hypocalcemia in patients with SHPT who undergo PTX, and used to construct predictive models. The AUCs for the constructed models were high for both the training (0.903) and test (0.948) groups. The calibration curve showed good agreement between the incidence of postoperative hypocalcemia estimated using the predictive model and the actual incidence. The DCA curve indicated that the predictive model performed well.ConclusionA predictive model constructed using a combination of preoperative PTH, Ca, and ALP may represent a useful means of identifying patients with SHPT at high risk of developing hypocalcemia following PTX in clinical practice.
Using loop-primer mediated PCR to enhance the detection of poorly preserved DNA
Authors: Xiang Hai , Wang Zhi , Yang Liu , Zhang Xing , Zhao Xingbo 
Published: November 2022

Ancient DNA is vitally important in evolutionary research, and obtaining authentic ancient DNA sequences is critical for a proper analysis. However, it is difficult to acquire amplicons accurately and efficiently from ancient DNA templates using current techniques. Here, we established a loop-primer-mediated amplification method (L-PCR) to obtain target ancient DNA sequences with high accuracy and efficiency. The method was tested using 66 ancient samples (including 27 pig bones or teeth and 39 chicken bones) and serially diluted modern animal DNA templates. Compared to nested PCR, L-PCR was proven to be more efficient and accurate and could obtain more amplicons from both ancient pig samples and chicken bones and detect as low as 10−3 ng/μl modern pig template DNA. The efficiency was at least 100-fold that of the nested PCR. The results suggest that L-PCR is advantageous for obtaining authentic DNA sequences from poorly preserved or recalcitrant ancient specimens.
No causal association between tea consumption and 7 cardiovascular disorders: A two-sample Mendelian randomization study
Authors: Cai Dongsheng , Chen Jun , Wu Yuteng , Jiang Chenyang 
Published: November 2022

Background: Previous studies have reported inconsistent results on the causal association between habitual tea consumption and the risk of cardiovascular disease (CVD). This study is aim to determine the association between habitual tea intake and CVD using two-sample Mendelian randomization (MR) analysis.Methods: The genetically predicted causation between tea consumption and 7 common cardiovascular diseases (atrial fibrillation, hypertension, acute myocardial infarction, coronary atherosclerosis, peripheral vascular disease, angina, and heart failure) was evaluated using MR analysis model. We performed a total of 9 MR analysis methods to analyze the final results. The IVW methods was used as the primary outcome. The other MR analysis method (simple mode, weighted mode, simple median, weighted median, penalized weighted median, MR Egger, and MR-Egger (bootstrap)) were performed as the complement to IVW. Also, the robustness of the MR analysis results was assessed using a leave-one-out analysis.Results: The IVW analysis methods indicated that there is no causal association between tea consumption and risk of CVD (AF: OR, 0.997, 95% CI, 0.992–1.0001, p = 0.142; hypertension: OR, 0.976, 95% CI, 0.937–1.017, p = 0.242; AMI: OR, 0.996, 95% CI, 0.991–1.000, p = 0.077; CA: OR, 1.001, 95% CI, 0.993–1.009, p = 0.854; PVD: OR, 1.002, 95% CI, 1.000–1.005, p = 0.096; angina: OR, 0.999, 95% CI, 0.993–1.006, p = 0.818; HF: OR, 0.999, 95% CI, 0.996–1.002, p = 0.338). The other MR analysis method and further leave-one-out sensitivity analysis suggested the results were robust.Conclusion: This MR study indicated that there was no genetically predicted causal association between habitual tea intake and risk of CVD.
Krebs von den lungen-6 as a clinical marker for hypersensitivity pneumonitis: A meta-analysis and bioinformatics analysis
Authors: He Jie , Zhang Jiangliu , Ren Xinyi 
Published: November 2022

AimHypersensitivity pneumonitis (HP), also referred to as exogenous allergic alveolitis, is one of the most common interstitial lung diseases (ILDs). A potential immune biomarker, Krebs von den lgen-6 (KL-6) characterizes the progression and severity of HP. The meta-analysis in this study was conducted to elucidate the variations in the concentrations of KL-6 in different types of HP.MethodsA systematic search of various databases such as EMBASE, Pubmed, CNKI, VIP, Web of Science, and WanFang was carried out to find relevant published articles between January 1980 and August 2022 that explored the relationship between KL-6 and allergic pneumonia. Standardized mean difference (SMD) and 95% confidence interval (CI) were used as effect sizes for comparison among different groups. The GSE47460 and GSE150910 datasets were downloaded to extract and validate the differences in KL-6 mRNA expression between HP lung tissue and healthy controls. Furthermore, the single-cell sequencing dataset GSE135893 was downloaded to extract KL-6 mRNA expression in type II alveolar epithelial cells to validate the differences between HP and healthy controls. Two researchers evaluated the quality of the included studies by employing Newcastle-Ottawa Scale. All the qualified studies were subjected to statistical analyses carried out utilizing RevMan 5.2, Stata 11.0, and R software 4.1.3.ResultsTwenty studies aligned perfectly with the inclusion criteria of the meta. The concentrations of KL-6 were substantially higher in the blood of HP patients as compared to the control group. Subgroup analyses were carried out in accordance with the allergen source and the results revealed that patients with different allergens had higher blood KL-6 concentrations than healthy controls. Additionally, different subgroups of subjects were created for meta-analysis as per the fibrosis status, race, measurement method, and sample type. The concentration of KL-6 in blood was much higher in all HP subgroups than in healthy control groups. Moreover, the bioinformatics analysis revealed that KL-6 mRNA expression was higher in HP lung tissue and type II alveolar epithelial cells as compared to healthy controls.ConclusionThe present meta-analysis and bioinformatics analysis suggested that the concentration levels of KL-6 varied between HP patients and healthy individuals, and the KL-6 concentrations may be higher in the blood samples of HP patients.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, CRD42022355334.
Crosstalk of four kinds of cell deaths defines subtypes of cutaneous melanoma for precise immunotherapy and chemotherapy
Authors: Wan Qi , Wei Ran , Wei Xin , Deng Ying-ping 
Published: November 2022

BackgroundCell death patterns can give therapeutic and biological clues that facilitate the development of individualized treatments for this lethal form of skin cancer.MethodsWe employed unsupervised clustering to establish robust classifications based on the four kinds of cell death-associated gene expression of 462 melanoma patients in the Cancer Genome Atlas (TCGA) and tested their reproducibility in two independent melanoma cohorts of 558 patients. We then used dimensionality reduction of graph learning to display the different characteristics of cell death patterns and immune microenvironments.ResultsWe examined 570 cell death-associated gene expression data of melanoma patients for exploration, independent verification, and comprehensive classification of five reproducible melanoma subtypes (CS1 to CS5) with different genomic and clinical features. Patients in death-inactive subtypes (CS1, CS2, and CS5) had the least immune and stromal cell infiltration, and their prognosis was the poorest. A death-active subtype (CS4), on the other hand, had the highest infiltrated immune and stromal cells and elevated immune-checkpoints. As a result, these patients had the highest response to immunotherapy and the best prognosis. An additional subtype (CS3) had more diversified cell death and immune characteristics with moderate prognoses. Based on graph learning, we successfully divided the CS3 subtype into two subgroups (group A and group B) with distinct survival outcomes and immune features. Finally, we identified eight potential chemical drugs that were specifically targeted for the therapy of melanoma subtypes.ConclusionsThis research defines the intrinsic subtypes of melanoma based on the crosstalk of four kinds of cell deaths, which affords a blueprint for clinical strategies and guiding precise immunotherapy and chemotherapy for melanoma patients.
Anti-glutathione S-transferase theta 1 antibodies correlate with graft loss in non-sensitized pediatric kidney recipients
Authors: Comoli Patrizia , Cioni Michela , Ray Bryan , Tagliamacco Augusto , Innocente Annalisa , Caridi Gianluca , Bruschi Maurizio , Hariharan Jayasree , Fontana Iris , Trivelli Antonella , Magnasco Alberto , Nocco Angela , Klersy Catherine , Muscianisi Stella , Ghiggeri Gian Marco , Cardillo Massimo , Verrina Enrico , Nocera Arcangelo , Ginevri Fabrizio 
Published: November 2022

IntroductionImmunity to Human leukocyte antigen (HLA) cannot explain all cases of ABMR, nor the differences observed in the outcome of kidney recipients with circulating DSAs endowed with similar biologic characteristics. Thus, increasing attention has recently been focused on the role of immunity to non-HLA antigenic targets.MethodsWe analyzed humoral auto- and alloimmune responses to the non-HLA antigen glutathione S-transferase theta 1 (GSTT1), along with development of de novo (dn)HLA-DSAs, in a cohort of 146 pediatric non-sensitized recipients of first kidney allograft, to analyze its role in ABMR and graft loss. A multiplex bead assay was employed to assess GSTT1 antibodies (Abs).ResultsWe observed development of GSTT1 Abs in 71 recipients after transplantation, 16 with MFI &gt; 8031 (4th quartile: Q4 group). In univariate analyses, we found an association between Q4-GSTT1Abs and ABMR and graft loss, suggesting a potential role in inducing graft damage, as GSTT1 Abs were identified within ABMR biopsies of patients with graft function deterioration in the absence of concomitant intragraft HLA-DSAs. HLA-DSAs and GSTT1 Abs were independent predictors of graft loss in our cohort. As GSTT1 Ab development preceded or coincided with the appearance of dnHLA-DSAs, we tested and found that a model with the two combined parameters proved more fit to classify patients at risk of graft loss.DiscussionOur observations on the harmful effects of GSTT1Abs, alone or in combination with HLA-DSAs, add to the evidence pointing to a negative role of allo- and auto-non-HLA Abs on kidney graft outcome.
Three-dimensional ultrasound assessment of risk factors for cystocele and Green classification in primipara
Authors: Yin Weiwei , Ma Qianqing , Xie Wen , Zhu Yuting , Wang Junli 
Published: November 2022

Background and aimsThe present study aimed to analyze the effects of factors on cystocele and the Green classification.Materials and methodsWe conducted a cross-sectional study on 357 primiparous women examined at our hospital from January 2019 to May 2021. The following data were recorded: maternal characteristics, neonatal characteristics, and factors of childbirth. It was added to the multivariate logistic regression model to determine the independent predictors of the cystocele and the Green classification.ResultsA total of 242 women had cystocele, including 71 women with Green type I cystocele, 134 women with Green type II cystocele, and 37 women with Green type III cystocele. In multivariate logistic regression analysis, body mass index (BMI) at delivery was associated with cystocele, while BMI at delivery and the second stage of labor (SSL) &gt; 1 h were independently with the distance from the symphysis pubis to the bladder neck (SPBN) abnormal (P &lt; 0.05). BMI at examination was associated with the large retrovesical angle (RVA) (P &lt; 0.05). BMI at delivery and the fetal right occiput anterior position (ROA) were independently associated with the distance from the symphysis pubis to the posterior wall of the bladder (SPBP) abnormal (P &lt; 0.05), while epidural anesthesia (EDA) was the protective factor (P &lt; 0.05).ConclusionPrimipara women should strive to avoid exposure to modifiable risk factors such as controlling weight during pregnancy, reducing weight after delivery, and shortening SSL to reduce the occurrence of cystocele.
Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs
Authors: Chang Zhenyu , Bo Suxue , Xiao Qingqing , Wang Yu , Wu Xi , He Yuxuan , Iqbal Mujahid , Ye Yourong , Shang Peng 
Published: November 2022

IntroductionThe establishment of intestinal microbiota and the maintenance of its equilibrium structure plays an important role in Tibetan pigs during different growth stages. Understanding the structure and function of the intestinal microbiota at different growth stages of Tibetan pigs can provide a theoretical basis for guiding nutritional regulation and feeding management in different stages.MethodsFecal samples were collected from the Tibetan piglets at different growth stages, and the 16S rRNA was sequenced to analyze the changes of intestinal microbiota.ResultsAlpha and Beta diversity indexes showed that the diversity of the intestinal microbiota did not change during the three growth stages, and the main components of intestinal microbiota were not significantly different. At the phylum level, Firmicutes and Bacteroidetes were dominant and abundant at different growth stages and were not restricted by age. At the genus level, Streptococcus, Lactobacillus, and Bifidobacterium were the most dominant in the TP10d and TP40d groups, Streptococcus was the most dominant in the TP100d group, followed by Treponema_2 and Lactobacillus. Fusobacteria, Gluconobacter, and Synergistetes were found to be specific genera of 10-day-old Tibetan piglets by LEfSe combined with LDA score. The change of diet made Tenericutes and Epsilonbacteraeota, which are closely related to digestive fiber, become specific bacteria at the age of 40 days. With the consumption of oxygen in the intestine, obligate anaerobes, such as Verrucomicrobia, Fibrobacter, and Planctomycetes, were the characteristic genera of 100 days. KEGG function prediction analysis showed that the intestinal microbiota function of Tibetan pigs changed dynamically with the growth and development of Tibetan piglets.DiscussionIn conclusion, the structure and composition of the intestinal microbiota of Tibetan pigs are significantly different at different growth and development stages, which plays an important role in their immune performance.
Topology of diffusion changes in corpus callosum in Alzheimer's disease: An exploratory case-control study
Authors: Kumar Sumeet , De Luca Alberto , Leemans Alexander , Saffari Seyed Ehsan , Hartono Septian , Zailan Fatin Zahra , Ng Kok Pin , Kandiah Nagaendran 
Published: November 2022

AimThis study aims to assess the integrity of white matter in various segments of the corpus callosum in Alzheimer's disease (AD) by using metrics derived from diffusion tensor imaging (DTI), diffusion kurtosis imaging (DKI) and white matter tract integrity model (WMTI) and compare these findings to healthy controls (HC).MethodsThe study was approved by the institutional ethics board. 12 AD patients and 12 HC formed the study population. All AD patients were recruited from a tertiary neurology memory clinic. A standardized battery of neuropsychological assessments was administered to the study participants by a trained rater. MRI scans were performed with a Philips Ingenia 3.0T scanner equipped with a 32-channel head coil. The protocol included a T1-weighted sequence, FLAIR and a dMRI acquisition. The dMRI scan included a total of 71 volumes, 8 at b = 0 s/mm2, 15 at b = 1,000 s/mm2 and 48 at b = 2,000 s/mm2. Diffusion data fit was performed using DKI REKINDLE and WMTI models.Results and discussionWe detected changes suggesting demyelination and axonal degeneration throughout the corpus callosum of patients with AD, most prominent in the mid-anterior and mid-posterior segments of CC. Axial kurtosis was the most significantly altered metric, being reduced in AD patients in almost all segments of corpus callosum. Reduced axial kurtosis in the CC segments correlated with poor cognition scores in AD patients in the visuospatial, language and attention domains.