bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardioprotective Effect of Polymyxin‐B and Dantrolene Combination on Isoproterenol‐Induced Hypertrophic Cardiomyopathy in Rats, via Attenuation of Calmodulin‐Dependent Protein Kinase II
Authors: Shaikh Faiza , Bhatt Lokesh Kumar 
Published: August 2022

AbstractHypertrophic cardiomyopathy is a major cause of mortality worldwide. In this study, we hypothesized that the combination of Dantrolene and Polymyxin‐B will provide cardioprotective action against isoproterenol‐induced hypertrophic cardiomyopathy via attenuation of Calmodulin‐dependent protein kinase II (CaMKII). Hypertrophic cardiomyopathy was induced in rats by subcutaneous administration of isoproterenol (5 mg/kg) for 14 days. Simultaneously, animals were treated with Polymyxin‐B per se, Dantrolene per se, and Dantrolene and Polymyxin‐B combination for 14 days. Hemodynamic parameters, biochemical parameters, and histological analysis were performed. Administration of isoproterenol for 14 days resulted in severe myocardial damage, characterized by cardiac hypertrophy and increase serum CK‐MB, CK‐Nac, LDH, AST, and ALT levels. It also caused alteration in electrocardiogram and blood pressure. A significant increase in CaMKII was observed in heart homogenate. Treatment with the Polymyxin‐B and Dantrolene combination significantly ameliorated cardiac hypertrophy, biochemical parameters, ECG parameters, and heart histopathology. Further, significant attenuation in CaMKII levels was observed. The effect of the combination was more than per se treatment. Results of the current study showed that the combination of Polymyxin‐B and Dantrolene prevented the development of isoproterenol‐induced hypertrophic cardiomyopathy in rats via attenuation of the CaMKII.
Essential Oils from Some Lamiaceae Plants: Antioxidant and Anticancer Potentials besides Thermal Properties
Authors: Kilinç Büşra Özlem , Gödelek Duygu , Süfer Özge , Saygideğer Demir Burcu , Sezan Aycan , Saygideğer Yasemin , Bozok Fuat 
Published: August 2022

AbstractThe purpose of this study was to determine the chemical compositions, antioxidant and anticancer activities and thermal behavior of essential oils (EOs) obtained by a microwave assisted Clevenger apparatus fromMentha longifoliasubsp.typhoidesvar.typhoides(ML),Thymus kotschyanusvar.glabrescens(TK),Calamintha nepetasubsp.nepeta(CN) andSatureja cuneifolia(SC) in Osmaniye, Turkey. Nepetalactone (34.23 %), thymol (37.40 %), piperitone oxide (27.25 %), and carvacrol (28.34 %) were major compounds in the EOs of ML, TK, CN, and SC. Total phenolic content and antioxidant activity (by FRAP assay) were in the range of 0.27–3.01 mg gallic acid equivalents and 0.62–171.14 μmol trolox equivalent per g EO, respectively. IC50values of DPPH were mostly greater than ABTS. IC50levels of the EOs of ML, TK, CN for the cytotoxic activities were 195.7, 265.7, 442.9 μg/ml, and 218.4, 204.2, 133.9 μg/ml for 24 and 48 h, respectively. IC50of SC‐EO could not be calculated in the applied concentration range. The highest fusion enthalpies were in between 58.72 and 81.65 kJ/kg. Both the TK and SC plant EOs had comparable and significant bioactivities. CN‐EO reduced cell motility and triggered apoptosis more effectively than the others.
Sustainable Selective Propanol Production via Continuous Flow Conversion of Glycerol over Synergistic Bifunctional Catalysts: An Exploration into Factors Affecting Activity
Authors: Folkard Aaron L. , Farahani Majid D. , Mahomed Abdul S. , Friedrich Holger B. 
Published: August 2022

AbstractSelective hydrogenolysis of glycerol to propanol is a sustainable reaction, which can play a critical role in the future of the propylene market, as one step dehydration of this sustainable propanol provides access to sustainable propylene. In this work, Copper (Cu) – Tungstosilicic acid (HSiW) catalysts were studied for the hydrogenolysis of glycerol. For the first time over a Cu catalyst, this study reports a yield of ∼76 % for direct conversion of glycerol to propanols in continuous flow mode. Several advanced characterization techniques were employed to study the fresh and used catalysts. The presence of intact Keggin units of HSiW solid acid (with medium acidity) in the vicinity of small clusters of copper sites improved the performance and lifetime of these bifunctional catalysts. This study offers insights into the nature of the active sites required for the hydrogenolysis reaction, which was achieved without using expensive PGMs.
Biotechnological Advances in Detection of Contaminants from Wastewater
Authors: Mehta Hinal , Patel Priya , Mukherjee Anwesha , Munshi Nasreen S. 
Published: August 2022

AbstractWastewater from industries causes major environmental pollution problems. The urban development and commercialization in developing countries cause deterioration of surface water resources. Hence, for its improvement, it is essential to develop highly sensitive devices with quick response for monitoring of wastewater contamination. In this respect, biotechnological processes such as biosensors can be promising and a robust option for detection of toxic pollutants. Biosensors can be applied in order to acquire real‐time quantitative data. Nowadays, the enzymes or whole cell bio‐reporters‐based biosensors have promising potential due to the possibility of miniaturization and opportunity for development of in situ biosensors. The growing need of convenient, rapid, and efficient biosensing devices explains the current advancement of biosensors with new transduction materials for multiplexed contaminant detection. Considering the extensive range of sensing strategies including immunochemical‐based, enzymatic and nonenzymatic signals, different kinds of biosensors can be classified as electrochemical, optical, thermal, etc. For the past few years, more attention has been paid to nanomaterial‐based biosensors which exhibit monitoring on the basis of chemical reactions and biological phenomena. Overall, this review focuses on principle and operation (transduction mechanism) of biosensors based on bio‐recognition elements, for the early detection of organic and inorganic contaminants existing in wastewater.
Evaluation of Anticancer Activity of 1,3‐Oxazol‐4‐ylphosphonium Salts in Vitro
Authors: Brusnakov Mykhailo , Golovchenko Olexandr , Velihina Yevheniia , Liavynets Oleksandr , Zhirnov Victor , Brovarets Volodymyr 
Published: August 2022

AbstractA novel series of 1,3‐oxazol‐4‐yltriphenylphosphonium salts has been synthesized and functionalized. Oxazole derivatives were subjected to NCI in vitro assessment. Seven most active derivatives have been selected for five‐dose assay. Among them, compounds 9 ([2‐(4‐methylphenyl)‐5‐[(4‐methylphenyl)sulfanyl]‐1,3‐oxazol‐4‐yl]triphenylphosphonium perchlorate), 1 ([5‐(4‐methylphenyl)amino]‐2‐phenyl‐1,3‐oxazol‐4‐yl]triphenylphosphonium perchlorate) and 4 ([5‐phenyl‐2‐[(4‐methylphenyl)amino]‐1,3‐oxazol‐4‐yl]triphenylphosphonium perchlorate) were the most active against all tested cancer subpanels. Statistical analysis of the total panel data showed average values of parameters of anticancer activity in the range of 0.3–1.1 μM (GI50), 1.2–2.5 μM (TGI) and 5–6 μM (LC50). It was found that the presence of phenyl or 4‐methylphenyl groups at C(2) and C(5) in the oxazole ring is of critical importance for the manifestation of the anticancer activity. Matrix COMPARE analysis using LC50 vector showed a high positive correlation of compound 9 with standard anticancer agents that can directly disrupt mitochondrial function, causing programmed death of cancer cells. The obtained results indicate the anticancer activity of 1,3‐oxazol‐4‐ylphosphonium salts, which could be useful for developing new anticancer drugs. The most active of them can be recommended for further in‐depth studies and synthesis of new derivatives with antitumor activity on their basis.
Fluorinated Cycloalkyl Building Blocks for Drug Discovery
Authors: Grygorenko Oleksandr O. , Melnykov Kostiantyn P. , Holovach Serhii , Demchuk Oleksandr 
Published: August 2022

AbstractThe review covers various aspects of fluorinated cycloalkyl (C3−C7) building blocks for drug discovery, including their synthesis, key physicochemical properties, and biological and medicinal applications of their derivatives. The discussed synthetic methods include classical nucleophilic fluorinations of various substrates, the addition of fluorine and another heteroatom to double bonds, cycloadditions and other transformations of fluorine‐containing substrates, as well as some newer reactions like fluorination of non‐activated and remotely activated C−H bonds, decarboxylative and deborylative fluorinations, etc. The known data on the effect of introducing the fluorinated cycloalkyl groups on the compound's key in vitro parameters (such as acidity/basicity, lipophilicity, conformational behavior, and short contact capabilities) are surveyed. Finally, applications of fluorinated cycloalkyl building block derivatives in the design of biologically active compounds (including marketed drugs Maraviroc, Ivosidenib, and Sitafloxacin) are covered, with a focus on the fluorination impact.
Discovery of an L‐like Configuration for 3’‐Fluoro‐5’‐norcarbonucleoside Phosphonates as Potent Anti‐HIV Agents
Authors: Geant Pierre‐Yves , Kaci Malika , Uttaro Jean‐Pierre , Périgaud Christian , Mathé Christophe 
Published: August 2022

AbstractRecently, we reported the racemic synthesis of 3’‐fluoro‐5’‐norcarbocyclic nucleoside phosphonates bearing adenine as the heterocyclic base. For this study, to evaluate the antiviral activity of each enantiomer, we synthesized both enantiomers, as well as their corresponding bis(POM) prodrugs. Anti‐HIV‐1 evaluation against the LAI strain and clinically NRTI‐resistant HIV‐1 strains are presented. The activities against these different strains show that the activities of bis(POM) prodrug (−)‐9 are equivalent or even superior to those of (R)‐PMPA.
Neuropeptide S (NPS) neurons: Parabrachial identity and novel distributions
Authors: Huang Dake , Zhang Richie , Gasparini Silvia , McDonough Miriam C. , Paradee William J. , Geerling Joel C. 
Published: August 2022

AbstractNeuropeptide S (NPS) increases wakefulness. A small number of neurons in the brainstem express Nps. These neurons are located in or near the parabrachial nucleus (PB), but we know very little about their ontogeny, connectivity, and function. To identify Nps‐expressing neurons within the molecular framework of the PB region, we used in situ hybridization, immunofluorescence, and Cre‐reporter labeling in mice. The primary concentration of Nps‐expressing neurons borders the lateral lemniscus at far‐rostral levels of the lateral PB. Caudal to this main cluster, Nps‐expressing neurons scatter through the PB and form a secondary concentration medial to the locus coeruleus (LC). Most Nps‐expressing neurons in the PB region are Atoh1‐derived, Foxp2‐expressing, and mutually exclusive with neurons expressing Calca or Lmx1b. Among Foxp2‐expressing PB neurons, those expressing Nps are distinct from intermingled subsets expressing Cck or Pdyn. Examining Nps Cre‐reporter expression throughout the brain identified novel populations of neurons in the nucleus incertus, anterior hypothalamus, and lateral habenula. This information will help focus experimental questions about the connectivity and function of NPS neurons.
Prediction and forecasting of worldwide corona virus (COVID‐19) outbreak using time series and machine learning
Authors: Jain Priyank , Sahu Shriya 
Published: August 2022

SummaryHow will the newly discovered coronavirus (COVID‐19) affect the world and what will be its global impact? For answering this question, we will require a prediction of overall recoveries and fatalities, as well as a reliable prognosis of coronavirus cases. Predicting, however, requires an ample total of past data related to it. On any particular day, the prediction is unclear since events in the future rarely repeat themselves the way that they did in the past. Furthermore, forecasts and predictions are determined by the absolute interests, accuracy of the data, and prophesied variables. In addition, psychological factors play an enormous role in how people perceive and react to the danger from the disease and therefore the fear that it is going to affect them personally. This research paper advances an unbiased method for predicting the increase of the COVID‐19 employing a simple, but powerful method to do so. Assumed that the data are accurate and reliable which the longer term will still follow an equivalent disease pattern, our projections intimate with a large association. Within the COVID‐19 cases were documented, in contingency, there is a steady increase. The hazards are far away from symmetric, as underestimating a pandemic's spread and failing to do enough to prevent it is far a lot worse than overspending and being too cautious when it will not be needed. This paper illustrates the timeline of a live forecasting study with huge implied implications for devising and decision‐making and gives unbiased predictions on COVID‐19 confirmed cases, recovered cases, deaths, and ongoing cases are shown on a continental map using data science and machine learning (ML) approaches. Utilizing these ML‐based techniques, the proposed system predicts the accurate COVID‐19 cases and gives better performance.
Corporate social responsibility and green behaviour: Towards sustainablefood‐businessdevelopment
Authors: Shiri Nematollah , Jafari‐Sadeghi Vahid 
Published: August 2022

AbstractLiterature in the field of corporate social responsibility (CSR) has mainly focused on customers, particularly on their loyalty, behavioural intent and satisfaction. That is, the effectiveness of CSR dimensions has been less considered by scholars. Hence, this research strives to explore the effect of CSR dimensions on the green behaviour among employees of food businesses in Iran. In this vein, the mediating role of green trust and the moderating role of gender in the relationship between the CSR dimensions and the green behaviour of employees have been investigated. The samples of this research included 415 employees of food businesses and companies in Iran. The findings reveal that the CSR dimensions (economic CSR, ethical CSR and philanthropic CSR) directly and indirectly—through the mediating variable of green trust—played an important role in shaping the green behaviour of employees. In this study, the direct and indirect effects of legal CSR on green behaviour among employees were not confirmed. Furthermore, the results represent that the gender variable moderates the effect of the CSR dimensions on the green behaviour of employees, highlighting that economic CSR and ethical CSR played a prominent role in shaping the green behaviour of respectively males and females. In addition, the theoretical and practical implications of the research discussed the impact of green behaviour on producing environmentally friendly products in order to sustainable development in the food business.
Influence of early social isolation on general activity and spatial learning in adult WAG/Rij rats
Authors: Shishelova Anna Y. , Smirnov Kirill , Raevskiĭ Vladimir V. 
Published: August 2022

AbstractThe study identifies the critical period of early ontogeny, during which social factors have the greatest influence on the operant behavior with positive reinforcement in adult WAG/Rij rats. Individual social isolation of rats from dam and siblings was performed daily for 3 h during postnatal day (PND) 2–8, 9–15, and 16–22. General activity and water consumption were examined using the IntelliCage (IC) in adulthood. The operant behavior training was performed in four consecutive sessions: free exploration of the IC environment (adaptation), learning to retrieve water by nosepoking (nosepoke adaptation), spatial learning to retrieve water in the specific corner (place learning), and retraining with a change of a place preference (reversal learning). Social isolation during PND16–22 led to the greatest behavioral changes in all sessions of the experiment. These rats were more active, consumed more water, demonstrated a higher ratio of visits with drinking to the total number, and relearned faster after changing the location of the rewarded corner. Thus, the postnatal period between days 16 and 22 in WAG/Rij rat pups is more sensitive to social isolation for change of adaptive behavior in the IC in adulthood.
Engineering current collectors for advanced alkali metal anodes: A review and perspective
Authors: Hu Liang , Deng Jiaojiao , Liang Qinghua , Wu Junxiong , Ge Bingcheng , Liu Qiang , Chen Guohua , Yu Xiaoliang 
Published: August 2022

AbstractAlkali metal batteries (AMBs) are promising next‐generation high‐density electrochemical energy storage systems. In addition, current collectors play important roles in enhancing their electrochemical performances. Thus, it is essential to have a critical review of the most recent advances in engineering the current collectors for high‐performance AMBs. In this review paper, the fundamentals of alkali metal deposition on current collectors will be introduced first. Then recent advances in the development of advanced metal and carbon‐based current collectors are examined for boosting the stability and cycle life of lithium metal batteries (LMBs) in terms of various strategies including 3D architectural design and functional modifications. Thereafter, the research progress in design of advanced current collectors will be analyzed for sodium/potassium metal batteries, especially the counterparts that do not follow the paradigms established in LMBs. Finally, the major challenges and key perspectives will be discussed for the future development of current collectors in AMBs.image
Mapping the effect of plant‐based extracts on immune and tumor cells from a bioactive compound standpoint
Authors: Huang Chujun , Xu Tianxiong , Li Yanping , Ren Jiaoyan 
Published: August 2022

AbstractInstead of traditional regimen of “one molecule, one target, one disease,” natural plant extracts, as a multi‐component system, show great potential in alleviate disease with complex etiology and pathophysiological factors, such as cancer. In this study, we evaluated the chemical characteristic as well as bioactive activities profiles of 15 species of plant‐based extracts, which corresponding to polysaccharide, protein, polyphenol content, and effects on MC38, LLC, RAW264.7 cell viability, respectively. Person correlation analysis and multi‐criteria decision analysis method was proposed to evaluate dose‐effect relationship and the comprehensive activities of each extract. A significant positive correlation was found between polyphenol value and immune cell activity, which means that it was considered to perform well on antitumor and immunomodulatory effects when compared to the studied substances. The subsequent measurement by a MC38‐N4/OT‐I coculture model verified the results, showing an excellent effect of Grifola frondosa on enhancing the antigen‐specific killing of tumor cells. Here we give a general view of the chemical composition and the bioactive activities of 15 plant‐based extracts and provide a feasible strategy to estimate the complex effect of phytochemicals. That would help us to better understand the effect of phytochemical combination and lay a good foundation for further in vivo studies.
Rapid in vivo multiplexed editing (RIME) of the adult mouse liver
Authors: Katsuda Takeshi , Cure Hector , Sussman Jonathan , Simeonov Kamen P. , Krapp Christopher , Arany Zoltan , Grompe Markus , Stanger Ben Z. 
Published: August 2022

Background and Aims:
Assessing mammalian gene function in vivo has traditionally relied on manipulation of the mouse genome in embryonic stem cells or perizygotic embryos. These approaches are time‐consuming and require extensive breeding when simultaneous mutations in multiple genes is desired. The aim of this study is to introduce a rapid in vivo multiplexed editing (RIME) method and provide proof of concept of this system.

Approach and Results:
RIME, a system wherein CRISPR/caspase 9 technology, paired with adeno‐associated viruses (AAVs), permits the inactivation of one or more genes in the adult mouse liver. The method is quick, requiring as little as 1 month from conceptualization to knockout, and highly efficient, enabling editing in >95% of target cells. To highlight its use, we used this system to inactivate, alone or in combination, genes with functions spanning metabolism, mitosis, mitochondrial maintenance, and cell proliferation.

Conclusions:
RIME enables the rapid, efficient, and inexpensive analysis of multiple genes in the mouse liver in vivo.
Zinc oxide nanoclusters and their potential application as CH4 and CO2 gas sensors: Insight from DFT and TD‐DFT
Authors: Muz İskender , Kurban Mustafa 
Published: August 2022

AbstractWe have investigated the adsorption of CH4 and CO2 gases on zinc oxide nanoclusters (ZnO NCs) using density functional theory (DFT). It was found that the CH4 tends to be physically adsorbed on the surface of all the ZnO NCs with adsorption energy in the range −11 to −14 kcal/mol. Even though, the CO2 is favorably chemisorbed on the Zn12O12 and Zn15O15 NCs, with adsorption energy about −38 kcal/mol at B3LYP/6‐311G(d,p) level of theory. When the CH4 and CO2 gases are adsorbed on the ZnO NCs, their electrical conductivities are decreased, and thus the studied ZnO NCs do not generate an electrical signal in the presence of CH4 and CO2 gases. Interestingly, both pure and gas adsorbed Zn22O22 NC exhibited more favorable electronic and reactive properties than other NCs. Comparison of the structural, electronic, and optical data predicted by DFT/B3LYP and TD‐DFT/CAM‐B3LYP calculations with those experimentally obtained show good agreement.
The diagnostic importance of multiple cytokines in adult hemophagocytic lymphohistiocytosis
Authors: Jin Zhili , Suolitiken Dina , Wang Yini , Wang Zhao 
Published: August 2022

AbstractBackgroundHemophagocytic lymphohistiocytosis (HLH) is a category of immunological illnesses that cause out‐of‐control T cells and macrophages to release life‐threatening cytokines. The HLH‐2004 diagnostic criteria are the gold standard for HLH diagnosis, but there is a need to investigate the usefulness of various cytokines for HLH diagnosis.MethodsPatients admitted to Beijing Friendship Hospital of Capital Medical University from January 2016 to December 2020 were included in this retrospective study, with 166 patients with confirmed HLH and 142 febrile patients requiring differential diagnosis completing the sum. Multiplex cytokine assays using multifactor liquid phase microarray technology‐based multifactor liquid phase microarray technology were used to detect 33 cytokines. Twenty‐eight cytokines detected using the Luminex analytical platform technology were ultimately included in the analysis.ResultsInterleukin‐1 receptor antagonist (IL‐1 RA), IL‐18, interferon‐γ (IFN‐γ), and interferon‐induced protein 10 (IP‐10) regulated upon activation normal T cell expressed and secreted (RANTES), eotaxin, growth‐related oncogene α (GRO‐α), and macrophage inflammatory protein‐1 α (MIP‐1α) were higher in the HLH group than in the non‐HLH group, and the differences were statistically significant. Among them, the area under the curve (AUC) for IL‐18 for HLH diagnosis was reported for the first time as 82.69%, with a sensitivity of 76.32% and a specificity of 79.61%; the AUC of IL‐1 RA was 72.34%, with a sensitivity of 62.71% and a specificity of 75.97%; and the AUC of IP‐10 was 71.73%, with a sensitivity of 60.14% and a specificity of 75.15%. Moreover, the AUC of the combined diagnostic tests for IL‐1 RA, IL‐18, IFN‐γ, IP‐10, and RANTES was 99.6%, with a sensitivity of 95.8% and a specificity of 98.6%.ConclusionOur study concluded that multiple cytokines are valid biological markers for the diagnosis of HLH. The findings of this study remain to be validated in an external dataset.