bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-time monitoring of serotonin with highly selective aptamer-functionalized conducting polymer nanohybrids
Authors: Lim Seong Gi , Seo Sung Eun , Park Seon Joo , Kim Jinyeong , Kim Yejin , Kim Kyung Ho , An Jai Eun , Kwon Oh Seok 
Published: July 2022

AbstractAdequate serotonin levels are pivotal to human well-being; thus, serotonin can be used as a biomarker because it regulates a wide range of physical and psychological functions. As an imbalance of serotonin is highly likely to initiate the pathogenesis of various disorders, monitoring serotonin levels in real time is in high demand for the early detection of disease. We fabricated a field-effect transistor (FET) biosensor based on aptamer-immobilized conducting polymer nanohybrids, which showed an instantaneous response toward serotonin in solution. The mechanism of serotonin detection was based on aptamer deformation after aptamer-ligand interaction and the consequential decrease in the charge carrier density of the FET template. Docking simulations with AutoDock/Vina and PyMOL were successfully used to investigate the binding site of serotonin in the loop structure of the aptamer. The fabricated FET template showed high sensitivity toward serotonin in the range of 10 fM to 100 nM, and the limit of detection (LOD) was exceptionally low at 10 fM. Moreover, the selectivity toward serotonin was confirmed by observing no signal after the injection of structural analogs, functional analogs and excess physiological biomolecules. The potential clinical application of this sensor was confirmed because it remained consistent when the buffer solution was exchanged for artificial serum or artificial cerebrospinal fluid (CSF). †S.G.L. and S.E.S. contributed equally to this work.
Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum
Authors: Schlemmer Timo , Lischka Richard , Wegner Linus , Ehlers Katrin , Biedenkopf Dagmar , Koch Aline 
Published: July 2022

AbstractNumerous reports have shown that incorporating a double-stranded RNA (dsRNA)-expressing transgene into plants or applying dsRNA by spraying it onto their leaves successfully protects them against invading pathogens exploiting the mechanism of RNA interference (RNAi). How dsRNAs or siRNAs are transferred between donor host cells and recipient fungal cells is largely unknown. It is speculated that plant extracellular vesicles (EVs) function as RNA shuttles between plants and their pathogens. Recently, we found that EVs isolated from host-induced gene silencing (HIGS) or spray-induced gene silencing (SIGS) plants contained dsRNA-derived siRNAs. In this study, we evaluated whether isolated EVs from dsRNA-sprayed barley (Hordeum vulgare) plants affected the growth of the phytopathogenic ascomycete Fusarium graminearum. Encouraged by our previous finding that dropping barley-derived EVs on F. graminearum cultures caused fungal stress phenotypes, we conducted an in vitro growth experiment in microtiter plates where we co-cultivated F. graminearum with plant EVs isolated from dsRNA-sprayed barley leaves. We observed that co-cultivation of F. graminearum macroconidia with barley EVs did not affect fungal growth. Furthermore, plant EVs containing SIGS-derived siRNA appeared not to affect F. graminearum growth and showed no gene silencing activity on F. graminearum CYP51 genes. Based on our findings, we concluded that either the amount of SIGS-derived siRNA was insufficient to induce target gene silencing in F. graminearum, indicating that the role of EVs in SIGS is minor, or that F. graminearum uptake of plant EVs from liquid cultures was inefficient or impossible.
Rescue percutaneous transhepatic portal vein embolization after failed associated liver partition and portal vein ligation for staged hepatectomy in a patient with multiple liver metastases of rectal cancer: a case report
Authors: Tomida Hidenori , Notake Tsuyhosi , Shimizu Akira , Kubota Koji , Umemura Kentaro , Kamachi Atsushi , Goto Takamune , Yamazaki Shiori , Soejima Yuji 
Published: July 2022

Abstract
Background
Liver metastasis is the most common form of distant spread of colorectal cancer. Despite oncological and surgical advances, only about 25% of patients are eligible to undergo resection. As the liver has a limited resectable volume, tumor reduction and remnant liver hypertrophy are of critical importance in treating initially unresectable colorectal cancer liver metastasis. Associated liver partition and portal vein ligation for staged hepatectomy (ALPPS) allows rapid liver hypertrophy within a short period and has been reported to be useful in recent years.

Case presentation
A 29-year-old woman complaining of bloody stool was referred to our hospital. She was diagnosed with rectal cancer (Rb) with simultaneous multiple liver and lung metastases. The patient was then initially commenced on chemotherapy and completed it with a satisfactory response. Right trisectionectomy was necessary to achieve hepatic clearance; however, the future liver remnant (FLR) volume was insufficient. Therefore, we decided to perform totally laparoscopic ALPPS to obtain enough FLR volume. However, the FLR increase was slow, and FLR did not attain the required volume for right trisectionectomy. Computed tomography showed that right portal venous blood flow was increased via developed collateral vessels around the portal vein. We attempted to induce further liver growth by blocking portal blood flow using additional percutaneous transhepatic portal vein embolization (PTPE), and a rapid increase in FLR was obtained. The patient underwent right trisectionectomy and partial resection of S2 with negative margins, and the patient was discharged without postoperative liver failure.

Conclusions
Resumption of the portal venous blood flow through collateral vessels after ALPPS may have interfered with the planned residual liver hypertrophy. Performing PTPE in addition to ALPPS increased the FLR volume, and radical hepatectomy was completed safely. Remnant portal venous blood flow following ALPPS is an important issue to be considered in surgical planning, and early additional portal vein embolization could be effective.
Postoperative outcomes of mepivacaine vs. bupivacaine in patients undergoing total joint arthroplasty with spinal anesthesia
Authors: Stock Laura A. , Dennis Kevin , MacDonald James H. , Goins Andrew J. , Turcotte Justin J. , King Paul J. 
Published: July 2022

Abstract
Background
Spinal anesthesia (SA) has been previously associated with improved outcomes after total joint arthroplasty (TJA). The purpose of this study was to compare outcomes between various local anesthetics.

Methods
This was a retrospective study of 1,328 patients undergoing primary TJA with SA from September 2020–2021 at a single institution. Patients were grouped based on TKA or THA and further separated and analyzed in terms of anesthetic agents—mepivacaine (M), hyperbaric bupivacaine (HB), or isobaric bupivacaine (IB). Subgroup analysis of same-day-discharge (SDD) patients and low- (<11 mg) and high-dose bupivacaine was performed. Statistical significance was assessed at P<0.05.

Results
Mepivacaine use was associated with younger age, lower ASAs, and lower Charlson Comorbidity Index (CCI) scores in both THAs and TKAs. Postoperatively, significant differences were found between HB, IB, and M in LOS, the first PT ambulation distance and rates of SDD, and home discharge in both THAs and TKAs. No significant differences in outcomes were observed between high- and low-dose bupivacaine in THAs or TKAs. In SDD patients, a significant difference was found only in the first 6-clicks mobility scores. After controlling for age, BMI, sex, ASA, and procedure type, mepivacaine was found to be associated with shorter LOS, increased likelihood of SDD, home discharge, POD-0 ambulation, and, further, the first ambulation distance. No significant differences were observed in 6-clicks mobility scores, urinary retention, 30-day ED returns or 30-day readmissions.

Conclusions
Both bupivacaine and mepivacaine are safe and effective local anesthetics for patients undergoing TJA as evidenced by low, similar rates of urinary retention and 30-day ED returns and readmissions. Mepivacaine does appeared to facilitate early ambulation, shorter LOS and home discharge and should be considered as the local anesthetic of choice for patients undergoing rapid recovery TJA.
Longitudinal Monitoring of Circulating Tumor DNA to Predict Treatment Outcomes in Advanced Cancers
Authors: Gouda Mohamed A. , Huang Helen J. , Piha-Paul Sarina A. , Call S. Greg , Karp Daniel D. , Fu Siqing , Naing Aung , Subbiah Vivek , Pant Shubham , Dustin Derek J. , Tsimberidou Apostolia M. , Hong David S. , Rodon Jordi , Meric-Bernstam Funda , Janku Filip 
Published: July 2022

PURPOSE The response to cancer therapies is typically assessed with radiologic imaging 6-10 weeks after treatment initiation. Circulating tumor DNA (ctDNA), however, has a short half-life, and dynamic changes in ctDNA quantity may allow for earlier assessment of the therapeutic response. METHODS Patients with advanced solid tumors referred to the Department of Investigational Cancer Therapeutics at The University of Texas MD Anderson Cancer Center were invited to participate in a liquid biopsy protocol for which serial blood samples were collected before, during, and after systemic therapy. We isolated ctDNA from serially collected plasma samples at baseline, mid-treatment, and first restaging. Genomically informed droplet digital polymerase chain reaction (ddPCR) was performed, and ctDNA quantities were reported as aggregate variant allele frequencies for all detected molecular aberrations. RESULTS We included 204 patients receiving 260 systemic therapies. The ctDNA detection rate was higher in progressors (patients with progressive disease) compared with nonprogressors (patients with stable disease, partial responses, or complete responses) at all time points ( P < .009). Moreover, ctDNA detection was associated with a shorter median time-to-treatment failure ( P ≤ .001). Positive delta and slope values for changes in ctDNA quantity were more frequent in progressors ( P ≤ .03 and P < .001, respectively) and were associated with a shorter median time-to-treatment failure ( P ≤ .014 and P < .001, respectively). Increasing ctDNA quantity was predictive of clinical and/or radiologic progressive disease in 73% of patients (median lead time, 23 days). CONCLUSION Detection of ctDNA and early dynamic changes in its quantity can predict the clinical outcomes of systemic therapies in patients with advanced solid tumors.
Effect of Liraglutide Treatment on Whole-body Glucose Fluxes in C-peptide–Positive Type 1 Diabetes During Hypoglycemia
Authors: Zenz Sabine , Regittnig Werner , Boulgaropoulos Beate , Augustin Thomas , Brunner Martina , Korsatko Stefan , Münzker Julia , Narath Sophie H , Raml Reingard , Magnes Christoph , Pieber Thomas R 
Published: July 2022

Abstract

Context
The effect of liraglutide in C-peptide–positive (C-pos) type 1 diabetes (T1D) patients during hypoglycemia remains unclear.

Objective
To investigate the effect of a 12-week liraglutide treatment on the body glucose fluxes during a hypoglycemic clamp in C-pos T1D patients and its impact on the alpha- and beta-cell responses during hypoglycemia.

Design
This was a randomized, double-blind, crossover study. Each C-pos T1D patient was allocated to the treatment sequence liraglutide/placebo or placebo/liraglutide with daily injections for 12 weeks adjunct to insulin treatment, separated by a 4-week washout period.

Setting and Participants
Fourteen T1D patients with fasting C-peptide ≥ 0.1 nmol/L.

Intervention(s)
All patients underwent a hyperinsulinemic-stepwise-hypoglycemic clamp with isotope tracer [plasma glucose (PG) plateaus: 5.5, 3.5, 2.5, and 3.9 mmol/L] after a 3-month liraglutide (1.2 mg) or placebo treatment.

Main Outcome Measure(s)
The responses of endogenous glucose production (EGP) and rate of peripheral glucose disposal (Rd) were similar for liraglutide and placebo treatment during the clamp.

Results
The numbers of hypoglycemic events were similar in both groups. At the clamp, mean glucagon levels were significantly lower at PG plateau 5.5 mmol/L in the liraglutide than in the placebo group but showed similar responses to hypoglycemia in both groups. Mean C-peptide levels were significantly higher at PG-plateaus 5.5 and 3.5 mmol/L after liraglutide treatment, but this effect was not reflected in EGP and Rd. Hemoglobin A1c and body weight were lower, and a trend for reduced insulin was seen after liraglutide treatment.

Conclusions
The results indicate that 3 months of liraglutide treatment does not promote or prolong hypoglycemia in C-pos T1D patients.
Cardiac Complications After 3-Column Osteotomy for Adult Spinal Deformity Patients With Formal Cardiac Clearance: Identifying Key Risk Factors and Threshold Cutoffs Via Recursive Partitioning Analysis
Authors: Lau Darryl , Haddad Alexander F. , Joshi Rushikesh S. , Deviren Vedat , Ames Christopher P. 
Published: July 2022

BACKGROUND:
Despite formal cardiac clearance, a subset of 3-column osteotomy (3CO) patients still experience cardiac complications (CCs).

OBJECTIVE:
To define the incidence and risk factors for CC in 3CO patients who had formal cardiac clearance and assess the utility of the Revised Cardiac Risk Index (RCRI) and preoperative metabolic equivalent (MET) functional ability in predicting perioperative CC.

METHODS:
Patients with adult spinal deformity (ASD) who underwent 3CO deformity correction from 2006 to 2019 were retrospectively reviewed. Multivariate and recursive partitioning analyses were performed to assess risk factors.

RESULTS:
A total of 390 patients with ASD were included. The mean age was 64.6 years, and 60.3% were female. The CC rate was 9.7%. Patients with CCs were older (P < .001), had an increased history of heart disease (P = .001), and higher blood loss (P = .045). RCRI score (P = .646) or MET functional ability (P = .493) were not associated with CC. On multivariate analysis, age (P < .001), blood loss (P = .008), and prior spinal fusion (P = .025) were independent risk factors for CC. Patients age older than 81 years had a significantly higher CC rate than those younger than 81 years. In patients age 81 years and younger, if blood loss was >3900 mL, CC rate was significantly higher. Among patients age 81 years and younger and with >3900 mL blood loss, CC rate is significantly higher in patients with ejection fraction (EF) ≤54.5%.

CONCLUSION:
RCRI and MET functional ability are limited risk assessment tools in ASD 3CO patients with formal cardiac clearance. Patients older than 81 years are at high risk for CC. In younger patients, cardiac EF and blood loss are significant components to risk stratify for CC.
Co-transport of the nuclear-encoded Cox7c mRNA with mitochondria along axons occurs through a coding-region-dependent mechanism
Authors: Cohen Bar , Altman Topaz , Golani-Armon Adi , Savulescu Anca F. , Ibraheem Amjd , Mhlanga Musa M. , Perlson Eran , Arava Yoav S. 
Published: July 2022

ABSTRACT
Nuclear-encoded mitochondrial protein mRNAs have been found to be localized and locally translated within neuronal processes. However, the mechanism of transport for those mRNAs to distal locations is not fully understood. Here, we describe axonal co-transport of Cox7c with mitochondria. Fractionation analysis and single-molecule fluorescence in situ hybridization (smFISH) assay revealed that endogenous mRNA encoding Cox7c was preferentially associated with mitochondria in a mouse neuronal cell line and within mouse primary motor neuron axons, whereas other mRNAs that do not encode mitochondrial protein were much less associated. Live-cell imaging of MS2-tagged Cox7c mRNA further confirmed the preferential colocalization and co-transport of Cox7c mRNA with mitochondria in motor neuron axons. Intriguingly, the coding region, rather than the 3′ untranslated region (UTR), was the key domain for the co-transport. Our results reveal that Cox7c mRNA can be transported with mitochondria along significant distances and that its coding region is a major recognition feature. This is consistent with the idea that mitochondria can play a vital role in spatial regulation of the axonal transcriptome at distant neuronal sites.
The Tunable Coupling between Metasurface and Molecular Vibration towards the Platform of Spectral Analysis
Authors: Nishijima Yoshiaki , Juodkazis Saulius 
Published: July 2022

Abstract
We have demonstrated the coupling of metasurface resonance with a molecular vibration from weak to strong. Metal-insulator-metal (MIM) metasurfaces using various polymers as the insulator layer (I-layer) were fabricated and characterised. The strength of coupling between MIM metasurface and molecular absorbance in the I-layer of organic nano-film depends on the dipole mode of the molecular vibration and the molecular number density. In the case of strong coupling, when Rabi-splitting is observed at the molecular absorption band, the coupling efficiency reaches several percent of the particular molecular vibration modes in the volume below nanodisks of the MIM metasurface.
Individual Educational Route As a Means of Developing Digital Competence of University Library Staff
Authors: Babina Ol'ga , Ermolovich E. 
Published: July 2022

The problem of determining the structure and content of the professional competence of library specialists in the context of digitalization is becoming more and more urgent and does not find its solution due to the lack of a valid professional standard for a specialist in library and information activities. The need to clearly describe the structure of the digital competence of the modern librarian, as well as how to develop it, determined the problems of this article.
The methodological and theoretical basis of this study was compiled by leading theories and scientific schools on a competent approach, set forth in the works of domestic and foreign scientists.
The paper describes the structure of digital competence of the university library staff, which includes cognitive, functional-activity, motivation-value and communicative components. The organizational and methodological conditions for the development of digital competence among the staff of the university library are justified and described. The authors developed the contents of educational routes built taking into account the specifics of the digital activities of the categories of university employees.
Prediction of spontaneous onset of labor at term (PREDICT study): Research protocol
Authors: Migliorelli Federico , Ferrero Ludovica , McCarey Catherine , Marcenaro Sara , Othenin-Girard Véronique , Chilin Antonina , Martinez de Tejada Begoña 
Published: July 2022

Background
Recent studies have shown that elective induction of labor versus expectant management after 39 weeks of pregnancy result in lower incidence of perinatal complications, while the proportion of cesarean deliveries remains stable, or even decreases. Still, evidence regarding collateral consequences of the potential increase of induction of labor procedures is still lacking. Also, the results of these studies must be carefully interpreted and thoroughly counter-balanced with women’s thoughts and opinions regarding the active management of the last weeks of pregnancy. Therefore, it may be useful to develop a tool that aids in the decision-making process by differentiating women who will spontaneously go into labor from those who will require induction.

Objective
To develop a predictive model to calculate the probability of spontaneous onset of labor at term.

Methods
We designed a prospective national multicentric observational study including women enrolled at 39 weeks of gestation, carrying singleton pregnancies. After signing an informed consent form, several clinical, ultrasonographic, biophysical and biochemical variables will be collected by trained staff. If delivery has not occurred at 40 weeks of pregnancy, a second visit and evaluation will be performed. Prenatal care will be continued according to current hospital guidelines. Once recruitment is completed, the information gathered will be used to develop a logistic regression-based predictive model of spontaneous onset of labor between 39 and 41 weeks of gestation. A secondary exploration of the data collected at 40 weeks, as well as a survival analysis regarding time-to-delivery outcomes will also be performed. A total sample of 429 participants is needed for the expected number of events.

Conclusion
This study aims to develop a model which may help in the decision-making process during follow-up of the last weeks of pregnancy.

Trial registration
NCT05109247 (clinicaltrials.gov).
Utility of hematological and inflammatory biomarkers in predicting recovery in critical Covid-19 patients: Our experience in the largest Covid-19 treating center in Lebanon
Authors: Salame Hassan , Nawfal Rashad , Kassem Jad , Mckey Remy , Kassem Ali , AlKhalil Nayef , Saleh Mohamad , Abdel Sater Ali H. , Ibrahim Ali , Abou-Abbas Linda , Eldbouni Oussaima , Khatoun Hoda , Matar Bassam 
Published: July 2022

Background
COVID-19 pandemic has led to a catastrophic shortage of ICU beds. This has resulted in the need to identify patients that can be discharged early before full clinical recovery. We designed this study to determine if in changes routine tests like CBCD and CRP can be a useful complement to clinical status when deciding to discharge patients from ICU.

Methods
This retrospective study was conducted in Rafic Hariri University Hospital. Levels of biomarkers measured at admission (T1) and within 3 days of outcome (T2) were collected and ratios (T2/T1) were calculated. The Odds Ratios of association between the changes in these biomarkers and outcome were estimated. Multivariate analysis and AUC for the performance of these biomarkers were also conducted.

Results
We found on multivariate analysis that reduction in counts of lymphocyte and platelets and elevation in counts of neutrophils and level of CRP (T2/T1 ratio > 1) are strongly associated with mortality with respective ORs estimated at 6.74, 3.26, 5.65 and 4.34 [p-values < 0.001]. AUCs were found to lie in a range of 0.68 to 0.81 indicating fair to good performance. Other factors found to impact survival were AKI, AF and ACS [p-values < 0.01]. In contrast to other studies, risk factors didn’t show an association with survival when adjusted for effects of complications and changes in biomarker levels.

Conclusions
Our results confirm that inexpensive tests like lymphocyte count and CRP can be reliably used to follow COVID-19 patients in ICU and to support the decision to discharge patients.
história paulista do Brasil
Authors: Giarola Flávio 
Published: July 2022

Nosso artigo analisa o manual didático A Linda História de Meu País, de César Martinez, publicado em 1929. Nosso objetivo é perceber como o autor procurou colocar São Paulo como protagonista da história nacional e como sinônimo do progresso do Brasil. Para isso, utilizou símbolos como os bandeirantes, para defender o papel dos paulistas na construção da nação; valorizou personagens e fatos históricos de São Paulo, em detrimento de outros tradicionalmente expostos na história escolar; e, ao falar do presente, defendeu o estado como centro do desenvolvimento da nação. Em contrapartida, afirmou que seu livro era voltado para o patriotismo e para a valorização da nacionalidade. Concluímos que na obra ocorre a convivência de duas identidades, a regional e a nacional, que o autor buscou, ao invés de polarizá-las, conciliá-las de forma que a paulistanidade fosse vista como condutora da nacionalidade.