bluuebunny/crossref_metadata_2025_split huggingface dataset:
Entheseal Involvement in Spondyloarthritis (SpA) and Gout: An Ultrasound Comparative Study
Authors: Ventura-Ríos Lucio , Cazenave Tomas , Hernández-Díaz Cristina , Gallegos-Nava Selma , Gómez-Ruiz Citlallyc , Rosemffet Marcos , Silva-Luna Karina , Rodríguez-Henríquez Pedro , Vázquez-Mellado Janitzia , Casasola-Vargas Julio , Cruz-Arenas Esteban , de Miguel Eugenio M. 
Published: May 2022

ObjectiveTo compare the assessment of entheses in subjects with spondyloarthritis (SpA) with patients with gout by the Madrid Sonographic Enthesis Index (MASEI).MethodThis cross-sectional study includes videos of entheses evaluated by ultrasound (US) of 30 patients with SpA diagnosed according to the ASAS criteria and 30 patients with gout established by the presence of monosodium urate crystals. Entheses were evaluated for MASEI in 2 Institutes located in two different countries. Demographic and clinical data were registered. Total MASEI score, MASEI-inflammatory, and MASEI-chronic damage were analyzed. Comparisons between groups were obtained by chi-square test and Student's t-test. An inter-reading US reliability was realized.ResultsPatients with gout were older and had significantly more comorbidities than those with SpA. The total MASEI score was not significantly different among diseases (p = 0.07). MASEI-inflammatory was significantly more prevalent at the Achilles tendon in SpA, while the proximal patellar tendon was in gout. Power Doppler was higher in SpA compared to gout (p = 0.005). MASEI-chronic damage related to calcification/enthesophytes predominated in gout (p = 0.043), while calcaneal erosions did in SpA (p = 0.008). The inter-reader concordance was excellent (0.93, CI 95% 0.87–0.96, p = 0.001).ConclusionsSpA and gout similarly involve entheses according to MASE, however, some inflammatory and chronic lesions differ significantly depending on the underlying disease and tendon scanned.
Extent of Neck Dissection and Cervical Lymph Node Involvement in Oral Squamous Cell Carcinoma
Authors: Thoenissen Philipp , Heselich Anja , Deeg Stefanie , Al-Maawi Sarah , Tanneberger Anna , Sader Robert , Ghanaati Shahram 
Published: May 2022

IntroductionTumor resection combined with neck dissection (ND) or radiotherapy are established methods for the treatment of patients with oral squamous cell carcinoma (OSCC). However, the extent of ND can lead to postoperative complications. Therefore, for the first time, this study aims to identify lymph node involvement in OSCC performed in a bilateral systematic approach based on oncologic board meetings relying on presurgical magnetic resonance imaging (MRI) and computed tomography (CT).Materials and MethodsIn a retrospective single-center study, patients with primary OSCC resection and systematic ND performed in 4 different manners (MRND III bilateral, MRND III left and SND right, MRND III right, SND left, and SND bilateral) were examined. Lymph node involvement allocated to levels was evaluated depending on primary localization and T-stage.ResultsA total of 177 consecutive patients (mean age 63.64; 92 female, male 85) were enrolled in this study. A total of 38.98% showed cervical lymph node involvement, and metastases were found in levels 1–4. The distribution of positive lymph node metastases (n=190 LNs) was 39.47% in level 1, 38.95% in level 2, 10.53% in level 3, and 11.05% in level 4.DiscussionIn a cohort of OSCC patients with systematic bilateral ND, levels 1 and 2 had positive lymph node involvement, and no lymph node involvement was seen at level 5. Without any clinical or imaging suspicion, ND expanding 5-level MRND should be avoided regardless of the primary tumor localization, T-stage and intraoperative proof of cervical metastases.
Cell Senescence-Related Pathways Are Enriched in Breast Cancer Patients With Late Toxicity After Radiotherapy and Low Radiation-Induced Lymphocyte Apoptosis
Authors: Aguado-Flor Ester , Fuentes-Raspall María J. , Gonzalo Ricardo , Alonso Carmen , Ramón y Cajal Teresa , Fisas David , Seoane Alejandro , Sánchez-Pla Álex , Giralt Jordi , Díez Orland , Gutiérrez-Enríquez Sara 
Published: May 2022

BackgroundRadiation-induced late effects are a common cause of morbidity among cancer survivors. The biomarker with the best evidence as a predictive test of late reactions is the radiation-induced lymphocyte apoptosis (RILA) assay. We aimed to investigate the molecular basis underlying the distinctive RILA levels by using gene expression analysis in patients with and without late effects and in whom we had also first identified differences in RILA levels.Patients and MethodsPeripheral blood mononuclear cells of 10 patients with late severe skin complications and 10 patients without symptoms, selected from those receiving radiotherapy from 1993 to 2007, were mock-irradiated or irradiated with 8 Gy. The 48-h response was analyzed in parallel by RILA assay and gene expression profiling with Affymetrix microarrays. Irradiated and non-irradiated gene expression profiles were compared between both groups. Gene set enrichment analysis was performed to identify differentially expressed biological processes.ResultsAlthough differentially expressed mRNAs did not reach a significant adjusted p-value between patients suffering and not suffering clinical toxicity, the enriched pathways indicated significant differences between the two groups, either in irradiated or non-irradiated cells. In basal conditions, the main differentially expressed pathways between the toxicity and non-toxicity groups were the transport of small molecules, interferon signaling, and transcription. After 8 Gy, the differences lay in pathways highly related to cell senescence like cell cycle/NF-κB, G-protein-coupled receptors, and interferon signaling.ConclusionPatients at risk of developing late toxicity have a distinctive pathway signature driven by deregulation of immune and cell cycle pathways related to senescence, which in turn may underlie their low RILA phenotype.
Necroptosis-Related LncRNAs Signature and Subtypes for Predicting Prognosis and Revealing the Immune Microenvironment in Breast Cancer
Authors: Xu Yuhao , Zheng Qinghui , Zhou Tao , Ye Buyun , Xu Qiuran , Meng Xuli 
Published: May 2022

PurposeNecroptosis is a mode of programmed cell death that overcomes apoptotic resistance. We aimed to construct a steady necroptosis-related signature and identify subtypes for prognostic and immunotherapy sensitivity prediction.MethodsNecroptosis-related prognostic lncRNAs were selected by co-expression analysis, and were used to construct a linear stepwise regression model via univariate and multivariate Cox regression, along with least absolute shrinkage and selection operator (LASSO). Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to measure the gene expression levels of lncRNAs included in the model. Based on the riskScore calculated, we separated patients into high- and low-risk groups. Afterwards, we performed CIBERSORT and the single-sample gene set enrichment analysis (ssGSEA) method to explore immune infiltration status. Furthermore, we investigated the relationships between the signature and immune landscape, genomic integrity, clinical characteristics, drug sensitivity, and immunotherapy efficacy.ResultsWe constructed a robust necroptosis-related 22-lncRNA model, serving as an independent prognostic factor for breast cancer (BRCA). The low-risk group seemed to be the immune-activated type. Meanwhile, it showed that the higher the tumor mutation burden (TMB), the higher the riskScore. PD-L1-CTLA4 combined immunotherapy seemed to be a promising treatment strategy. Lastly, patients were assigned to 4 clusters to better discern the heterogeneity among patients.ConclusionsThe necroptosis-related lncRNA signature and molecular clusters indicated superior predictive performance in prognosis and the immune microenvironment, which may also provide guidance to drug regimens for immunotherapy and provide novel insights into precision medicine.
Association of Mitochondrial DNA Polymorphisms With Pediatric-Onset Cyclic Vomiting Syndrome
Authors: Veenin Kirana , Wattanasirichaigoon Duangrurdee , Suktitipat Bhoom , Noojarern Saisuda , Lertrit Patcharee , Tim-Aroon Thipwimol , Kaewsutthi Supannee , Treepongkaruna Suporn 
Published: May 2022

BackgroundCyclic vomiting syndrome (CVS) is a functional gastrointestinal disorder characterized by recurrent stereotypic episodes of vomiting. The pathophysiology of CVS remains obscure. Previous studies have supported the hypotheses of mitochondrial dysfunction. However, data on association studies between mitochondrial DNA (mtDNA) polymorphisms and pediatric-onset CVS are limited and inconsistent. The aims of this study were to describe clinical characteristics, evaluate association of mtDNA polymorphisms 16519T and 3010A with pediatric-onset CVS and identify new mtDNA candidate variants.MethodsThis study involved Thai patients diagnosed with CVS according to the Rome III or IV criteria before the age of 15 years. Patients' demographic data, clinical characteristics, previous investigations and treatment outcomes were obtained. Blood samples were collected for next-generation (whole exome) sequencing, followed by analysis of chromosome M (mitochondrial. Variants were filtered according to clinical significance using ClinVar and MITOMAP. mtDNA polymorphisms in 148 normal Thai individuals were used as controls.ResultsForty-eight children were enrolled in the clinical study, and 30 participated in the genetic analysis. The median age at onset and median age at diagnosis was 3.0 (1.5–5.6) and 6.3 (3.0–8.6) years, respectively. Maternal history of migraine was positive in 16.7%. About 45.7% (21 of 46) of the patients achieved complete clinical remission, with the mean symptom duration of 5.9 ± 3.3 years. The prevalence of mtDNA variants 16519T and 3010A among the patient group and Thai general population (control) were as follows: 40.0% (12/30) vs. 27.7% (P = 0.18) and 6.7% (2/30) vs. 0.7% (P = 0.07), respectively. Five known pathogenic variants were identified in 6 patients, including mtDNA 8528C in one patient who also had infantile hypertrophic cardiomyopathy. Six likely pathogenic variants were found but without statistical significance. We identified 11 variants with significant prevalence in the patient group. Though, these variants were classified as variants of unknown significance (VUS), several of them were located in mt functional regions and therefore they deserve further investigations as new candidates for association with pediatric CVS.ConclusionThere were no associations of mtDNA polymorphisms 16519T and 3010A with CVS in our pediatric cohort. Five pathogenic variants and 11 VUS were found associated with pediatric-onset CVS.
Rhoifolin Alleviates Alcoholic Liver Disease In Vivo and In Vitro via Inhibition of the TLR4/NF-κB Signaling Pathway
Authors: Mai Baoyu , Han Ling , Zhong Jiarui , Shu Jingqi , Cao Zelin , Fang Jiaqi , Zhang Xiaoying , Gao Zelin , Xiao Fengxia 
Published: May 2022

Background: Alcoholic liver disease (ALD) is a common chronic liver disorder worldwide, which is detrimental to human health. A preliminary study showed that the total flavonoids within Citrus grandis “Tomentosa” exerted a remarkable effect on the treatment of experimental ALD. However, the active substances of Citrus grandis “Tomentosa” were not elucidated. Rhoifolin (ROF) is a flavonoid component present in high levels. Therefore, this research aimed to evaluate the hepatoprotective effects of ROF and its possible mechanisms.Methods: Molecular docking was performed to analyze the binding energy of ROF to the main target proteins related to ALD. Subsequently, mice were fed ethanol (ETH) for 49 days to establish the chronic alcoholic liver injury models. The liver pathological injury, serum aminotransferase levels, and oxidative stress levels in the liver tissue were measured. Human normal hepatocytes (LO2 cells) were incubated with ETH to construct the alcoholic liver cell model. The inflammatory markers and apoptosis factors were evaluated using real-time PCR and flow cytometry. Finally, the effects of ROF on the CYP2E1 and NF-κB signaling pathways were tested in vitro and in vivo.Results: Molecular docking results demonstrated that ROF was able to successfully dock with the target proteins associated with ALD. In animal studies, ROF attenuated ETH-induced liver damage in mice by decreasing the serum concentrations of AST and ALT, reducing the expression of inflammatory cytokines, and maintaining antioxidant balance in the liver tissue. The in vitro experiments demonstrated that ROF suppressed ETH-induced apoptosis in LO2 cells by promoting Bcl-2 mRNA and inhibiting Bax mRNA and caspase 3 protein expression. ROF decreased the level of LDH, ALT, AST, ROS, and MDA in the supernatant; induced the activity of GSH and SOD; and inhibited TNF-α, IL-6, and IL-1β expression levels. Mechanistically, ROF could significantly downregulate the expression levels of CYP2E1, TLR4, and NF-κB phosphorylation.Conclusion: This study indicates that ROF is the active component within the total flavonoids, which may alleviate ETH-induced liver injury by inhibiting NF-κB phosphorylation. Therefore, ROF may serve as a promising compound for treating ALD.
Does Plasma Inhibit the Activity of KCl Cotransport in Red Cells From LK Sheep?
Authors: Lu David C.-Y. , Hannemann Anke , Gibson John S. 
Published: May 2022

Red cells from LK sheep represent an important paradigm for control of KCl cotransport activity, as well as being important to sheep erythroid function. A previous report (Godart et al., 1997) suggested that autologous plasma markedly inhibits red cell KCC activity and identified the presence of the bicarbonate/CO2 buffer system as the probable cause. Findings were restricted, however, to red cells from patients with sickle cell disease (SCD) swollen anisotonically and carried out at a very high O2 tension (c.700 mmHg). It was therefore important to investigate the generality of the effect described and whether it was also relevant to the two main stimuli for KCC activity encountered most often by circulating red cells in vivo - low pH in active muscle beds during exercise and high urea concentrations in the renal medulla during antidiuresis. Results confirm that inhibition was significant in response to anisotonic swelling with KCC activity in MOPS-buffered saline (MBS) vs. bicarbonate-buffered saline (BBS) and in MBS vs. plasma both reduced (by about 25 and 50%, respectively). By contrast, however, inhibition was absent at low pH and in high concentrations of urea. These findings suggest therefore that red cell KCC activity represents an important membrane permeability in vivo in red cells suspended in plasma. They are relevant, in particular, to sheep red cells, and may also be important by extension to those of other species and to the abnormal red cells found in human patients with SCD.
Spectroscopic and Physicochemical Studies on 1,2,4-Triazine Derivative
Authors: Alrooqi Arwa , Al-Amshany Zahra M. , Al-Harbi Laila M. , Altalhi Tariq A. , Refat Moamen S. , Hassanien Ali M. , Mersal Gaber A. M. , Atta Ahmed A. 
Published: May 2022

A novel 5-(5-Bromo-2-hydroxybenzylidene)-6-oxo-3-phenyl-5,6-dihydro-1,2,4-triazine-2(1H)-carbothioamide (4) “compound 4” was synthesized. The chemical structure of compound 4 was confirmed with spectroscopic techniques. Thermal analysis (TGA/dTGA) studies were conducted for identifying the kinetic thermodynamic parameters and the thermal stability of the synthesized compound 4. Cyclic voltammetric studies were performed for recognizing electrochemical characteristics of the synthesized compound 4. The calculated highest occupied molecular orbital (HOMO), lowest unoccupied molecular orbital (LUMO), and the band gap were found to be −3.61, −5.32, and 1.97 eV, respectively. Using a diffused reflectance spectroscopy (DRS) technique, the estimated values of the optical band transitions of compound 4 in powder form were found to be 2.07 and 2.67 eV. The structural properties of thermally evaporated compound 4 thin films were analyzed using field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. It was found that compound 4 has a triclinic crystal structure. The optical transitions and the optical dispersion factors of compound 4 thin films were investigated using a UV-Vis spectroscopy technique. From the UV-Vis spectroscopy technique, Egind=3.6 V was estimated for both the as-deposited and annealed thin films. For the as-deposited film, there were two photoluminescence (PL) emission peaks centered at 473 and 490 nm with a shoulder at 422 nm. For the annealed film at 423 K, there were five PL emission peaks centered at 274, 416, 439, 464, and 707 nm with a shoulder at 548 nm. The dark electrical conduction of compound 4 thin film was through a thermally activated process with activation energy equaling 0.88 eV.
Gestión digital para la calidad de servicio en atención de reclamos a usuarios de la EPS Marañón S.A-Jaén
Authors: Culqui Lozada Mirtha , Callao Alarcón Marcelino 
Published: May 2022

Para que las entidades públicas puedan optimizar su capacidad de respuesta ante las necesidades y exigencias de cada ciudadano, necesitan implementar la gestión digital en todas las oficinas y departamentos, porque, esto permite solventar peticiones y mejorar la calidad de vida de la población. Se utilizó como objetivo general analizar la construcción sobre un modelo de gestión digital que contribuya a la calidad de servicio en atención de reclamos a usuarios de la EPS Marañón S.A. Jaén, 2021. Asimismo, se utilizó un enfoque cuantitativo, con diseño no experimental transaccional, de tipo básica – propositivo. Para ello se consideró como técnica la encuesta y como instrumento el cuestionario, el cual, fue aplicado a 77 trabajadores de la EPS Marañón S.A. Llegando a concluir que la gestión digital, presenta demasiadas deficiencias, siendo el factor principal que afecta directamente a la calidad de servicio en la atención que se brinda a los usuarios.
Plan participación ciudadana para el fortalecimiento del sistema educativo descentralizado de la Institución Educativa
Authors: Quesquén Díaz Jenny 
Published: May 2022

La participación ciudadana y la descentralización educativa son procesos que han permitido mejorar los niveles de acceso y calidad educativa, pero inconclusos en los espacios locales o desde las instituciones educativas para lograr un mayor impacto significativo en la ciudadanía, por eso se planteó el objetivo de diseñar un plan de participación ciudadana que fortalezca el sistema educativo descentralizado en la Institución Educativa; se identificó procesos, entidades y funcionamiento de la participación ciudadana y analizó factores y procesos de la descentralización educativa. La investigación fue de tipo básica, pro, descriptiva – proyectiva, diseño no experimental; se aplicó como instrumento un cuestionario a 46 representantes de entidades de la Institución Educativa y se entrevistó a tres especialistas. Se obtuvo como resultado que la valoración de la participación ciudadana y descentralización educativa es baja (39.0% y 39,1%, respectivamente). Considerando que se obtuvo una valoración baja de las variables, se diseñó el plan de participación ciudadana para fortalecer el sistema educativo descentralizado de la Institución Educativa, con la finalidad de mejorar la gestión y calidad educativa, lograr autonomía educativa desde los espacios locales; se estructuró considerando las dimensiones de las variables, actores locales, aliados estratégicos y basado en las normas legales vigentes.
Indicadores de gestión en el manejo de los recursos públicos en la Municipalidad
Authors: Nuñez Jimenez Jessica Karina 
Published: May 2022

Los recursos públicos son todos aquellos que están relacionados con el gasto que realizan los gobiernos del Estado, con la finalidad de prestar servicios públicos de diversos tipos; sin embargo, se observa mucha deficiencia en el manejo de los recursos de la administración en la gestión pública en la Municipalidad. Es por ello que este estudio se propuso diagnosticar el manejo de los recursos públicos en la Municipalidad de Pimentel. Para ello se utilizó una investigación de tipo básica con enfoque de carácter cuantitativo con un diseño transversal no experimental. Asimismo, se usó una muestra con 66 funcionarios públicos aplicando muestreo probabilístico con una unidad de análisis. Para la cual se utiliza la técnica de la encuesta y el instrumento del cuestionario que fueron los mecanismos de medición aplicados en el presente trabajo. Entre los resultados que más destacan se tiene que la variable relacionada con las fuentes de financiamientos sobre los recursos públicos obtuvo un nivel bajo con el 61%, así mismo la variable gestión obtuvo también un bajo nivel con el 42%. Concluyendo que el costo/ beneficio dentro del modelo resulta factible y sobre todo viable.
Estrategia	de	gestión	pública	para	inclusión	de	personas	con discapacidad en la municipalidad
Authors: Zarpán Romero Rosa María 
Published: May 2022

En la actualidad hay un promedio de 480 millones de individuos que están en edad para trabajar y que presentan una discapacidad como mínimo; en el año 2006 la Organización de las Naciones Unidas puso en vigencia normas creadas por la Organización Internacional sobre los derechos para las personas con discapacidad; sin embargo, las instituciones del Estado peruano no acatan estas disposiciones, es el caso de la Municipalidad Distrital de Tumán. Razón por la cual, el presente estudio tuvo como propósito diagnosticar las políticas públicas para la inclusión de las personas con discapacidad que se desarrollan en la Oficina Municipal de Atención a las Personas con Discapacidad (OMAPED). El estudio fue realizado con enfoque cuantitativo, de nivel descriptivo. Para ello se tomaron a 92 personas identificadas por OMAPED, a quienes se les aplicó un cuestionario basado en la dirección, educación, cultura y capacitación e integración laboral. Los resultados mostraron que no existen políticas claras en beneficio de estas personas y son pocas las oportunidades de desarrollo, no existe un ambiente adecuado y tampoco acceso a servicios de salud básica para control médico. Se concluye que es importante la aplicación de estrategias de gestión pública para mejorar la inclusión partiendo de los ejes estratégicos como la cultura inclusiva laboral, medidas institucionales para el apoyo para trabajadores con discapacidad, actividades que promuevan y fortalezcan las relaciones interpersonales, el reclutamiento, selección y promoción.
Age-related menopause and carbonyl stress
Authors: Бричагина А.С. , Семёнова Н.В. , Колесникова Л.И. 
Published: May 2022

В обзоре проанализированы данные литературы некоторых исследований редокс-гомеостаза у женщин с возрастной физиологической менопаузой. Несмотря на неоднозначность представленных результатов по окислительной модификации липидов, белков, углеводов, большинство исследований позволяет рассматривать данный возрастной период как один из факторов развития карбонильного стресса, являющегося неотъемлемой частью старения. Наличие гипергликемии и свободнорадикальной патологии представлены как основные причины развития карбонильного стресса, возрастной дефицит эстрогенов с учетом их антиоксидантных свойств рассматривается в качестве одного из пусковых механизмов развития данного состояния, а система глутатиона определена как один из его основных ингибиторов.
The review analyzes the literature data on studies of redox homeostasis in women with age-related physiological menopause. Despite the ambiguity of the presented results on the lipids, proteins, carbohydrates oxidative modification, most studies allow us to consider this age period as one of the factors in the carbonyl stress development, which is an integral part of aging. The presence of hyperglycemia and free radical pathology are presented as the main causes of the carbonyl stress development, age-related estrogen deficiency, taking into account their antioxidant properties, is considered as one of the triggers for this condition development, and the glutathione system is identified as one of its main inhibitors.