bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular identification and phylogenetic characterization of new A-strain isolates of Maize streak virus from western Ethiopia
Authors: Ketsela Daniel , Oyeniran Kehinde Adewole , Bekele Berhanu , Fontenele Rafaela S , Kraberger Simona , Varsani Arvind 
Published: July 2022

Abstract
The A-strain of Maize streak virus (MSV) causes maize streak disease (MSD) which is a major biotic threat to maize production in sub-Saharan Africa. Previous studies have described different maize streak virus (MSV) strains of economic importance from southern and eastern African countries, and how eastern African regions are hubs for MSV diversification. Despite these efforts, and lack of extensive sampling, there is limited knowledge about the MSV-A diversity in Ethiopia. Here, field sampling of maize plants and wild grasses with visible MSD symptoms were carried out in western Ethiopian regions of Gambela, Oromia, and Benishangul-Gumuz, during the maize growing season of 2019. The complete genomes of MSV isolates (n = 60) were cloned and Sanger sequenced. We use a model based phylogenetic approach to analyse 792 full MSV genome sequences available in the GenBank together with newly recovered genomes from Ethiopia to determine their subtypes and recombinant lineages. Of the 127 fields accessed, MSD prevalence was highest at 96% in Gambela region, and lowest in Oromia at 66%. Highest mean symptom severity of 4/5, (where 5 is the highest and 1 the lowest) was observed for both Gambela and Benishangul-Gumuz. Our results show that these new MSV isolates belong in the recombinant lineage V of the A1 subtype with the widest dissemination, and economic significance in the sub-Saharan Africa and the adjacent Indian Ocean islands.
Curcumin ameliorates LPS/D-GalN induced acute liver injury by regulating TLR4/NF-кB/NLRP3 signaling pathway in vivo and in vitro
Authors: Gui Ying , Zhang Yunchao , Lei Huan , Deng Qin , Liu Ziyuan , Li Shuo , Qiu Yinsheng , Mei Zhinan , Xu Lingyun 
Published: July 2022

Abstract
Background: NLRP3 inflammasome activation results in liver inflammation and injury. Curcumin, a polyphenol from Curcumin longa, exhibits anti-inflammatory properties. The effects and anti-inflammatory mechanisms of curcumin were explored on acute liver injury induced by LPS/D-GalN.Methods and Results: An in vitro acute liver injury model was established on L-02 cells by treatment with10 μg/mL lipopolysaccharide (LPS); An in vivo model was established on SD rats by intraperitoneal injection of 0.02 mg/kg LPS and 500 mg/kg D-galactosamine (D-GalN). Biochemical index detection, histopathology, Western blotting, and qPCR were used to explore the effects and anti-inflammatory mechanisms of curcumin on acute liver injury induced by LPS/D-GalN in vitro and in vivo. Our results showed that curcumin significantly reduced the damage of L-02 cells induced by LPS, reduced the levels of transminase, inhibited oxidative stress induced by LPS in rats, and alleviated liver pathological injury. These effects accompanied by the downregulation of the expression of TLR4, NF-кB, and pyrotosis related proteins NLRP3 and caspase 1.Conclusions: Curcumin ameliorated acute liver injury induced by LPS and D-GalN in L-02 cells and SD rats by inhibiting TLR4/NF-кB/NLRP3 pathway.
Promising anti-Toxoplasma activity of 1,2,3-triazole tethering sulfonamide moiety; synthesis; characterization and in vitro study
Authors: Arafa Fadwa Mostafa , Osman Doaa Hassan , Tolba Mona Mohamed , Rezki Nadjet , Aouad Mohamed R , Hagar Mohamed , Osman Mervat , Said Heba 
Published: July 2022

Abstract
Background
Toxoplasmosis is an infection that prevails all over the world and is caused by the obligate intracellular protozoan parasite Toxoplasma gondii (T.gondii). The current study introduces new lead compounds for the treatment of T.gondii.
Methods
Three novel 1,2,3-triazoles-based sulfonamide scaffold were designed, synthesized and fully characterized in order to investigate the In vitro toxoplasmacidal potency using Vero cells as host for T. gondii. Selectivity index parameter was used to express compounds efficacy in the inhibition of T. gondii proliferation, whereas the scanning electron microscopy technique was used to assess the ultrastructural changes. A significant anti-toxoplasma activity was observed with hybrid molecules compared to the sulfadiazine as a positive control.
Results
The results showed that the IC50 (inhibitory concentration) of the investigated compounds 3(a,b,c) were recoded as 10.335 µg/ml, 20.106 µg/ml and 5.574 µg/ml, respectively; while the used sulfadiazine exhibited 49.794 µg/ml as IC50. The investigated 1,2,3-triazole-sulfadrug molecular conjugates 3(a,b,c) revealed selectivity index at 21.028, 8.356, 28.595, respectively. All compounds were highly selective than the gold standard drug which showed selectivity index of 7. Scanning electron microscopy (SEM) revealed that most tachyzoites had remarkable morphological changes after 2 hours of exposure.
Conclusions
These results indicate that these new candidates 3(a,b,c) have the potential to be a viable source of antiparasitic therapeutic agents, and compound 3c exhibits the highest activity against T. gondii.
Assessment of Patient Willingness to SMS based health promotion and its associated factors in Northern Ethiopia: A facility based cross-sectional study
Authors: Gebreslassie Tesfit Gebremeskel , Weldu Liya Mamo 
Published: July 2022

Abstract
Background: - The health system collaborating with mobile technology has created a mixed-expertise called mHealth. mHealth is about using mobile technology for treatment compliance, appointment reminders, community mobilization, and awareness-raising over health issues. Toll-free voice calls, downloadable third-party mobile applications, and bulk SMS are among the many different types of mHealth Initiatives. SMS is ubiquitous, lean, and with far-reaching technological capacity for infrastructure constraint setting. SMS-based health intervention mainly focuses on health education, self-management, health promotion, and health service provision. Different SMS-based health promotion implementation researches have been implemented in different parts of the globe. The findings of these researches conclude that SMS-based health promotion was efficacious towards addressing the intended purpose. But, according to the WHO observatory survey, raising awareness using mHealth initiatives have shown a low level of service uptake globally. Most of these researches were in developed countries, and taking these results ASIS and opting to implement it in low- and middle-income countries may have its own drawbacks. The purpose of this research is to assess patient willingness to SMS-based health promotion and identify its associated factors.Method: - Facility based cross-sectional study was used. Data were collected using adopted and customized questionnaire from previous studies. By means of single population proportion formula and simple random sampling technique, 400 Outpatient and inpatient visitors to Ayder comprehensive specialized hospital from September 2019 up to October 2020 were selected. SPSS v.26 was used to analyze descriptive statistics and associativity properties.Result: - Of all 400 participants, 343(85.75%) are willing to receive SMS intervention for health promotion. Though gender was not a moderator, education, age and settlement are associative factors for SMS based health promotion (P > 0.001). Most of Participants prefers to start the SMS service within one month (335(97.7)) and are in need of receiving the messages once a week (136(39.7%)) late in the evening (199 (58.0%)).Conclusion: The study showed that SMS willingness for health promotion was high. This study also revealed that the associated demographic factors such as Education and age should be given a special emphasis in designing and implementing SMS based intervention. Besides the type, mode and timing of the SMS service should be in line with the health intervention.
Anthropogenic microparticles in the emerald rockcod Trematomus bernacchii (Nototheniidae): a 1998 snapshot from the Antarctica
Authors: Bottari Teresa , Nibali Valeria Conti , Branca Caterina , Grotti Marco , Savoca Serena , Romeo Teresa , Spano Nunziacarla , Azzaro Maurizio , Greco Silvestro , D'Angelo Giovanna , Mancuso Monique 
Published: July 2022

Abstract
Anthropogenic microparticles (AMs) were found for the first time in specimens of Trematomus bernacchii collected in 1998 in the Ross Sea (Antarctica) and stored in the Antarctic Environmental Specimen Bank. Most of the identified AMs were fibers of natural and synthetic origin. The natural AMs were cellulosic, the synthetic ones were polyester, polypropylene, polypropylene/polyester, and cellulose acetate. The presence of dyes in the natural AMs indicates their anthropogenic origin. Five industrial dyes were identified by Raman spectroscopy with Indigo occurring in most of them (55%). Our research not only adds further data to the ongoing knowledge of pollution levels in the Antarctica ecosystem, it provides an interesting snapshot of the past, highlighting that microplastics and anthropogenic fiber pollution had already entered the Antarctic marine food web at the end of the ‘90s. These findings therefore establish the foundations for understand the changes in marine litter pollution over time.
The Correlation of Pericoronary Adipose Tissue with Coronary artery disease and left ventricular function
Authors: You Deshu , Yu Haiyang , Wang Zhiwei , Wei Xiaoyu , Wu Xiangxiang , Pan Changjie 
Published: July 2022

Abstract
Objective: To investigate the correlation of pericoronary adipose tissue with coronary artery disease and left ventricular function.Methods: Participants with clinically suspected coronary artery disease were enrolled. All participants underwent coronary computed tomography angiography (CCTA) and echocardiography followed by invasive coronary angiography (ICA) within 6month. pericoronary adipose tissue (PCAT) was extracted to analyze the correlation with Gensini score and left ventricular function parameter, including IVS, LVPW, LVEDD, LVESD, LVEDV, LVESV, FS, LVEF, LVM, LVMI. The correlation between PCAT and Gensini was assessed by using Spearman correlation analysis, and between PCAT volume or FAI and left ventricular function parameters was assessed by using partial correlation analysis.Results: One hundred and fifty-nine participants (mean age, 64.55years ±10.64, male:65.4%[104/159]) were included for final analysis. The risk factors of coronary artery disease, such as hypertension, diabetes, dyslipidemia, history of smoking and drinking, had no significant association with the PCAT (P>0.05); and there is no correlation between PCAT and Gensini score. However, the LAD-FAI was positively correlated with IVS(r=0.203, P=0.013), LVPW(r=0.218, P=0.008),LVEDD(r=0.317, P＜0.001)，LVESD(r=0.298, P＜0.001), LVEDV(r=0.317, P＜0.001), LVESV(r=0.301, P＜0.001), LVM(r=0.371, P＜0.001), LVMI(r=0.304, P＜0.001). The LCX-FAI was positively correlated with LVEDD(r=0.199, P=0.015)，LVESD(r=0.190, P=0.021), LVEDV(r=0.203, P=0.013), LVESV(r=0.197, P=0.016), LVM(r=0.220, P=0.007), LVMI(r=0.172, P=0.036). The RCA-FAI was positively correlated with LVEDD(r=0.258, P=0.002)，LVESD(r=0.238, P=0.004), LVEDV(r=0.266, P=0.001), LVESV (r=0.249, P=0.002), LVM(r=0.237, P=0.004), LVMI(r=0.218, P=0.008). The total volume was positively correlated with FS (r=0.167, P=0.042).Conclusion: The FAI was positively correlated with the left ventricular function, but was not associated with the severity of coronary artery disease.
Sweetness effect of stevioside in paraventricular thalamic enhanced the feeding motivation of mice
Authors: Zhu* Yingjie , Jiang Shaolei , Song Bo , Chen Gaowei , Shen Shuifa , Qian Wei-Liang , Liu Zhongdong 
Published: July 2022

Abstract
Attitudes towards the consumption of various types of sweetened food products are controlled by the central nervous system. Therefore, finding the brain regions responsible for regulating the feeding decisions on sweeteners in animals will provide new strategies for the development of sweeteners. Here, we selected sucrose and the other two plant sweeteners: stevioside and xylitol, then provided the mice with the diets supplemented with 25% sucrose, 1.25% stevioside and 25.05% xylitol, respectively, to ensure that these diets had the same sweetness. The mice showed strong preference for HSD (high sucrose diet) and HSSD (high stevioside diet), but they were not interested in HXD (high xylitol diet) with the same sweetness as HSD. After six weeks of intermittent exposure to different sweet diets, when the mice have consumed the corresponding diet, the c-fos expression level in paraventricular thalamic (PVT) of mice in HSD group is significantly higher than those in the HXD group (p < 0.0001) and CD group (p = 0.0006), and there was no significant difference from the HSSD group to HXD group (p = 0.0507). Though fiber photometry, we found that the PVT of mice were involved in the feeding motivation of different sweet diets. When the mice consumed sucrose solution, the neuronal activity of PVT increased rapidly and peaked at the instant when the mice obtained the sucrose solution, this phenomenon was also observed in the HSSD group of mice. The PVT activity of mice exposed to water and xylitol solutions has no fluctuation. Our data indicate that PVT is involved in the regulation of the feeding motivation of mice for different sweeteners, and stevioside can activate PVT like sucrose to increase the feeding motivation of animals.
Fragmentation Route of doubly ionized benzene, aniline, and nitroanilines monomers using a novel protocol from density functional theory and QTAIM
Authors: Oliveira Carlos X. , Costa Fabio L.P. , Mota Gunar V. S. 
Published: July 2022

Abstract
The possibility of finding the fragmentation routes by theoretical methods, led us to compare the molecular ions between neutral molecules of benzene, aniline, and o-, m-, and p-nitroaniline, using the Density Functional Theory (DFT), under an aug-cc-pVDZ base set and a B3LYP exchange-correlation functional. After determining the structure and electronic energy of neutral and doubly ionized species, we used a new protocol based on the analysis of Wiberg's binding indexes and the quantum theory of atoms in Bader molecules (QTAIM). Where the charge transfer and electronic distribution in aromatic monomers indicate the possibility of fragment formation in at least two pairs of carbon-carbon (CC) atoms and indicate the possible loss of the -CNH2 and -NO2 groups in the aniline and nitroaniline molecules doubly ionized.
Simultaneous Analysis of Nine Volatile Phenols in Smoked Meat Products Using an Gas Chromatography-Tandem Mass Spectrometry
Authors: LI Jun , WANG Zhen , LAI Fei , SHU MeiCheng , SONG Lei , HE ZhiLin 
Published: July 2022

Abstract
A sensitive method for the determination of nine volatile phenols in smoked meat products samples has been developed using Gas Chromatography-Tandem Mass Spectrometry(GC-MS/MS). Smoked meat sample was extracted with soaking in 0.1 mol/L sodium hydroxide solution for 12 hours.The extracting solution was acidified by injecting the excess CO2 gas and then purified using sodium chloride and acetonitrile. Good linearities (R2>0.9908) were acquired for the analytes. The limit of detection and quantification were 5–15 μg kg-1 for all the nine target compounds. The recoveries were 81.6–92.0% and the relative standard deviations values were lower than 8.2% at three spiked levels (50, 200 and 1000 μg kg-1 ). The results indicated that the method was employed for the determination of volatile phenolic compounds in smoked meat because of its accuracy, speediness, high-efficiency and inexpensiveness.
Projection of Meteorological Drought under a Changing Climate in the Urmia Lake Basin, Iran
Authors: Chelan Bagher Shirmohammadi , Rostami Maryam , Moosavi Vahid , Varamesh Saeid , Semiromi Majid Taie 
Published: July 2022

Abstract
Arid and semi-arid regions are more susceptible to climate change impacts, leading to recurrent and sustained meteorological droughts as a natural hazard. Therefore, this study is established to assess the meteorological drought characteristics in the Urmia Lake Basin under a changing climate in Iran. To that end, General Circulation Models (GCMs) were evaluated using observed and Global Precipitation Climatology Centre (GPCC) datasets. Then, the selected GCMs were applied to project drought conditions until 2046 under Representative Concentration Pathway 2.6 and 8.5 (RCP2.6 and RCP8.5). Afterward, we developed eight combined scenarios (A1, A2, A3, A4, B1, B2, B3, and B4) to assess future drought characteristics. Ultimately, the Standardized Precipitation Index (SPI) was calculated using the Drought Indices Package (DIP) for the historic (1985–2015) and future (2016–2046) periods under two temporal scales, namely the medium-term (SPI-6) and long-term (SPI-18). According to the results, precipitation is expected to increase from 16.3–34% while the northeast and south parts of the basin will be affected by future drought effects. The projected SPI-18 under RCP8.5 indicated that the basin will suffer from the most severe and long-standing meteorological drought, anticipated to happen around 2045–2046 (A4 scenario). The increase was projected for severe low-frequency drought events in A4 (long-term) and, B1 and A3 (medium-term) scenarios. While, in most cases, the decrease was shown for the high-frequency with less intensity drought events mainly in medium-term assessment.
New strategy for silicon supply through fertigation in sugarcane integrating the pre-sprouted seedling phase and field cultivation
Authors: Silva José Lucas Farias , Prado Renato Mello , Alves Thayane Leonel , Lata-Tenesaca Luis Felipe , Soares Mariana Bomfim 
Published: July 2022

Abstract
Adopting a Si supply strategy can amplify sugarcane response. Thus, this study aimed to verify whether Si supply in the pre-sprouted seedling (PSS) formation phase would have an effect after field transplanting similar to Si supply only in the field phase (via foliar spraying or fertigation), and whether Si supply in the PSS formation phase associated with Si fertigation after transplanting can potentiate or amplify Si benefits. Two experiments were conducted. In experiment I, PSS were grown in nursery without Si (Control) and with Si. Experiment II was conducted in the field on Eutrustox with the following treatments: no Si supply (Control); Si supplied during the PSS formation phase; Si supplied through foliar spraying in the field; Si supplied through fertigation in the field; and Si supplied in the PSS formation phase and during field development. Silicon use in both crop phases benefited sugarcane by increasing photosynthetic pigment content and the antioxidative defense system. The innovation of Si management to be supplied via fertigation integrated in both crop phases (PSS and in the field) optimizes the element use by increasing the crop's productivity and sustainability.
Development of Python Application for Automated Geomatics Data Processing, Analysis, and Visualisation
Authors: Ugwuanyi Ifeanyi A. , Anejionu Obinna C.D. 
Published: July 2022

Abstract
Emerging technologies have led to the rapid generation of vast quantities of field data by geomatics professionals. However, these raw field data cannot be directly utilised unless they undergo rigorous processing (using complex mathematical models e.g. - reductions, adjustments, and computations) to convert them to meaningful information. These operations are often repetitive. Despite global advancement in automated data processing, most surveyors in developing countries such as Nigeria still deploy manual surveying computation techniques for their day-to-day data processing and analysis needs. Hence, the development of cost effective and easy-to-use automated applications based on the existing models is highly relevant, to ease operations requiring repeated processes. This study developed a Python-based geomatics application “Survey Companion” that can streamline the processing of survey field data into meaningful spatial information. To achieve these, python codes were written for the processing of data from key geomatics operations such as traverse and levelling networks, resection, intersection, and bathymetric operations as well as area computation. It is expected that this application will greatly improve survey operations in developing countries as well as increase the speed of data processing, thereby reducing overall project costs.
m6A RNA Methylation Regulator-Based Signature for Prognostic Prediction and its Potential Immunological Role in Uterine Corpus Endometrial Carcinoma
Authors: Fang Fang , Wang Peipei , Huang Haoyu , Ye Mingquan , Liu Xiaocen , Li Qingqing 
Published: July 2022

Abstract
BackgroundUterine corpus endometrial carcinoma (UCEC) is the most common female pelvic malignancy worldwide. N6-methyladenosine (m6A) plays an important role in various cellular responses, especially in cancer progression. However, the correlation between prognostic UCEC and m6A RNA methylation regulators remains unclear. MethodsWe used The Cancer Genome Atlas (TCGA) to provide a gene signature that could improve the prognostic evaluation of UCEC patients according to the distinct genetic trait of m6A RNA methylation regulators from a bioinformatics perspective. After comparing UCEC subgroups with different genetic profiles of m6A regulators, we identified 71 differentially expressed genes associated with overall survival (OS) and generated a nine-gene signature through least absolute shrinkage and selection operator (LASSO) Cox regression analysis. Finally, we used in vitro tumor cell experiments and immune correlation analysis to verify the function of each gene in the proposed gene signature.ResultsTime-dependent receiver operating characteristic (ROC) curves revealed that the proposed gene signature could predict the outcome of UCEC patients accurately. We found that CDKN2A mainly acted from the perspective of tumor cells, while COL4A4, PXDN, TIGIT, CHODL, LMO3, KCNJ12, and L1CAM might play a role in UCEC from an immunological point of view.ConclusionsFrom an epigenetics perspective, the m6A RNA methylation regulator-based gene signature can predict the prognosis of UCEC patients and immune therapeutic efficacy.