bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipoprotein(a) Modulates Carotid Atherosclerosis in Metabolic Syndrome
Authors: Cremonini Anna Laura , Pasta Andrea , Carbone Federico , Visconti Luca , Casula Matteo , Elia Edoardo , Bonaventura Aldo , Liberale Luca , Bertolotto Maria , Artom Nathan , Minetti Silvia , Contini Paola , Verzola Daniela , Pontremoli Roberto , Viazzi Francesca , Viviani Giorgio Luciano , Bertolini Stefano , Pende Aldo , Montecucco Fabrizio , Pisciotta Livia 
Published: June 2022

Background and Aim: High lipoprotein(a) [Lp(a)] is a well-established cardiovascular (CV) risk factor, but the effect of mildly elevated Lp(a) on CV health is largely unknown. Our aim was to evaluate if Lp(a) is associated with the severity of carotid atherosclerosis (CA) in the specific subset of metabolic syndrome (MetS).Patients and Methods: Subjects with diagnosed MetS and ultrasound-assessed CA were enrolled. Those patients were categorized according to the severity of CA (moderate vs. severe), and the circulating levels of Lp(a) alongside with clinical, anthropometric, and biochemical data were collected.Results: Sixty-five patients were finally included: twenty-five with moderate and forty with severe CA (all with asymptomatic disease). Intergroup comparison showed Lp(a) as the only significantly different variable [6 (2–12) mg/dl vs. 11.5 (6–29.5) mg/dl; p = 0.018]. Circulating levels of Lp(a) were also confirmed as the only variable independently associated with severity of CA at logistic regression analysis [OR 2.9 (95% CI 1.1–7.8); p = 0.040]. ROC curve analysis for Lp(a) confirmed a serum level of 10 mg/dl as the best cut-off value [AUC 0.675 (95% CI 0.548–0.786)]. Although sensitivity and specificity were suboptimal (69.0 and 70.4%, respectively)—likely due to the small sample size—this result is in line with those previously reported in the literature.Conclusion: Lp(a) is independently associated with severity of CA in the subgroup of MetS patients.
Reaching the “Hard-to-Reach” Sexual and Gender Diverse Communities for Population-Based Research in Cancer Prevention and Control: Methods for Online Survey Data Collection and Management
Authors: Myers Katie J. , Jaffe Talya , Kanda Deborah A. , Pankratz V. Shane , Tawfik Bernard , Wu Emily , McClain Molly E. , Mishra Shiraz I. , Kano Miria , Madhivanan Purnima , Adsul Prajakta 
Published: June 2022

PurposeAround 5% of United States (U.S.) population identifies as Sexual and Gender Diverse (SGD), yet there is limited research around cancer prevention among these populations. We present multi-pronged, low-cost, and systematic recruitment strategies used to reach SGD communities in New Mexico (NM), a state that is both largely rural and racially/ethnically classified as a “majority-minority” state.MethodsOur recruitment focused on using: (1) Every Door Direct Mail (EDDM) program, by the United States Postal Services (USPS); (2) Google and Facebook advertisements; (3) Organizational outreach via emails to publicly available SGD-friendly business contacts; (4) Personal outreach via flyers at clinical and community settings across NM. Guided by previous research, we provide detailed descriptions on using strategies to check for fraudulent and suspicious online responses, that ensure data integrity.ResultsA total of 27,369 flyers were distributed through the EDDM program and 436,177 impressions were made through the Google and Facebook ads. We received a total of 6,920 responses on the eligibility survey. For the 5,037 eligible respondents, we received 3,120 (61.9%) complete responses. Of these, 13% (406/3120) were fraudulent/suspicious based on research-informed criteria and were removed. Final analysis included 2,534 respondents, of which the majority (59.9%) reported hearing about the study from social media. Of the respondents, 49.5% were between 31-40 years, 39.5% were Black, Hispanic, or American Indian/Alaskan Native, and 45.9% had an annual household income below $50,000. Over half (55.3%) were assigned male, 40.4% were assigned female, and 4.3% were assigned intersex at birth. Transgender respondents made up 10.6% (n=267) of the respondents. In terms of sexual orientation, 54.1% (n=1371) reported being gay or lesbian, 30% (n=749) bisexual, and 15.8% (n=401) queer. A total of 756 (29.8%) respondents reported receiving a cancer diagnosis and among screen-eligible respondents, 66.2% reported ever having a Pap, 78.6% reported ever having a mammogram, and 84.1% reported ever having a colonoscopy. Over half of eligible respondents (58.7%) reported receiving Human Papillomavirus vaccinations.ConclusionStudy findings showcase effective strategies to reach communities, maximize data quality, and prevent the misrepresentation of data critical to improve health in SGD communities.
The Application of Biopsy Density in Transperineal Templated-Guided Biopsy Patients With PI-RADS<3
Authors: Zhu Hai , Ding Xue-fei , Lu Sheng-ming , Ding Ning , Pi Shi-yi , Liu Zhen , Xiao Qin , Zhu Liang-yong , Luan Yang , Han Yue-xing , Chen Hao-peng , Liu Zhong 
Published: June 2022

BackgroundIn patients with multiparameter magnetic resonance imaging (mpMRI) low-possibility but highly clinical suspicion of prostate cancer, the biopsy core is unclear. Our study aims to introduce the biopsy density (BD; the ratio of biopsy cores to prostate volume) and investigates the BD-predictive value of prostate cancer and clinically significant prostate cancer (csPCa) in PI-RADS&lt;3 patients.MethodsPatients underwent transperineal template–guided prostate biopsy from 2012 to 2022. The inclusion criteria were PI-RADS&lt;3 with a positive digital rectal examination or persistent PSA abnormalities. BD was defined as the ratio of the biopsy core to the prostate volume. Clinical data were collected, and we grouped the patients according to pathology results. Kruskal–Wallis test and chi-square test were used in measurement and enumeration data, respectively. Logistics regression was used to choose the factor associated with positive biospy and csPCa. The receiver operating characteristic (ROC) curve was used to evaluate the ability to predict csPCa.ResultsA total of 115 patients were included in our study. Biopsy was positive in 14 of 115 and the International Society of Urological Pathology grade groups 2–5 were in 7 of all the PCa patients. The BD was 0.38 (0.24-0.63) needles per milliliter. Binary logistics analysis suggested that PSAD and BD were correlated with positive biopsy. Meanwhile, BD and PSAD were associated with csPCa. The ROC curve illustrated that BD was a good parameter to predict csPCa (AUC=0.80, 95% CI: 0.69-0.91, p&lt;0.05). The biopsy density combined with PSAD increased the prediction of csPCa (AUC=0.90, 95% CI: 0.85-0.97, p&lt;0.05). The cut-off value of the BD was 0.42 according to the Youden index.ConclusionIn PI-RADS&lt;3 patients, BD and PSAD are related to csPCa. A biopsy density of more than 0.42 needles per millimeter can increase the csPCa detection rate, which should be considered as an alternative biopsy method when we perform prostate biopsy in patients with PI-RADS&lt;3.
Modeling Kaempferol as a Potential Pharmacological Agent for COVID-19/PF Co-Occurrence Based on Bioinformatics and System Pharmacological Tools
Authors: Jiang Yong , Xie Yi-Zi , Peng Chen-Wen , Yao Kai-Nan , Lin Xue-Ying , Zhan Shao-Feng , Zhuang Hong-Fa , Huang Hui-Ting , Liu Xiao-Hong , Huang Xiu-Fang , Li Hang 
Published: June 2022

Objective: People suffering from coronavirus disease 2019 (COVID-19) are prone to develop pulmonary fibrosis (PF), but there is currently no definitive treatment for COVID-19/PF co-occurrence. Kaempferol with promising antiviral and anti-fibrotic effects is expected to become a potential treatment for COVID-19 and PF comorbidities. Therefore, this study explored the targets and molecular mechanisms of kaempferol against COVID-19/PF co-occurrence by bioinformatics and network pharmacology.Methods: Various open-source databases and Venn Diagram tool were applied to confirm the targets of kaempferol against COVID-19/PF co-occurrence. Protein-protein interaction (PPI), MCODE, key transcription factors, tissue-specific enrichment, molecular docking, Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to clarify the influential molecular mechanisms of kaempferol against COVID-19 and PF comorbidities.Results: 290 targets and 203 transcription factors of kaempferol against COVID-19/PF co-occurrence were captured. Epidermal growth factor receptor (EGFR), proto-oncogene tyrosine-protein kinase SRC (SRC), mitogen-activated protein kinase 3 (MAPK3), mitogen-activated protein kinase 1 (MAPK1), mitogen-activated protein kinase 8 (MAPK8), RAC-alpha serine/threonine-protein kinase (AKT1), transcription factor p65 (RELA) and phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) were identified as the most critical targets, and kaempferol showed effective binding activities with the above critical eight targets. Further, anti-COVID-19/PF co-occurrence effects of kaempferol were associated with the regulation of inflammation, oxidative stress, immunity, virus infection, cell growth process and metabolism. EGFR, interleukin 17 (IL-17), tumor necrosis factor (TNF), hypoxia inducible factor 1 (HIF-1), phosphoinositide 3-kinase/AKT serine/threonine kinase (PI3K/AKT) and Toll-like receptor signaling pathways were identified as the key anti-COVID-19/PF co-occurrence pathways.Conclusion: Kaempferol is a candidate treatment for COVID-19/PF co-occurrence. The underlying mechanisms may be related to the regulation of critical targets (EGFR, SRC, MAPK3, MAPK1, MAPK8, AKT1, RELA, PIK3CA and so on) and EGFR, IL-17, TNF, HIF-1, PI3K/AKT and Toll-like receptor signaling pathways. This study contributes to guiding development of new drugs for COVID-19 and PF comorbidities.
The Rapid and Long-Lasting Antidepressant Effects of Iridoid Fraction in Gardenia Jasminoides J.Ellis Are Dependent on Activating PKA-CREB Signaling Pathway
Authors: Ren Li , Zhang Hailou , Tao Weiwei , Chen Yin , Zou Zhilu , Guo XiaoYan , Shen Qinqin , Feng Quansheng , Hu Jingqing 
Published: June 2022

Lag periods of therapeutic efficacy cause poor compliance of patients, which has made solutions for rapid antidepressants the most urgent need in the depression study field at present. We have identified through our previous studies the rapid antidepressant effects of the traditional herb Gardenia jasminoides J.Ellis [Rubiaceae] (GJ) and its standardized fractions. Through screening different fractions of GJ, we decided to place our focus on the iridoid fraction of GJ (GJ-IF).Methods: 1. Tail suspension test (TST), forced swimming test (FST), and novelty suppressed-feeding test (NSFT) were performed in sequence on mice after GJ-IF administration. 2. Mice in the model group were under chronic unpredictable mild stress (CUMS) for 3 w. After GJ-IF treatment, mice were placed in an open field test (OFT), Sucrose preference test (SPT), NSFT, TST, and FST. 3. Western Blot was performed to examine the expression of brain-derived neurotrophic factor (BDNF), Synapsin 1, cyclic-AMP dependent protein kinase A (PKA), phosphorylated cyclic-AMP responsive element-binding protein (p-CREB), and cAMP response element-binding protein (CREB). 4. Mice in the test group were administrated with GJ-IF after intraperitoneal injection of PKA blocker H89.Results: 1. GJ-IF treatment significantly reduced the immobility time of TST at 1 d and FST at 26 h. 2. GJ-IF reversed the deficits induced by 3 w CUMS in SPT, TST, FST, and NSFT at 1 d and 26 h. The antidepressant effects of a single dose of iridoid fraction could also last for at least 14 d. 3. The results of molecule studies suggested that a single dose of GJ-IF activated p-CREB at 2 h and the PKA-CREB pathway at 1 d. The expression of BDNF did not significantly change from 30 min to 1 d after GJ-IF administration. 4. Blockade of PKA-CREB signaling pathway reversed the antidepressant effects of GJ-IF at 1 d, but not 30 min and 2 h.Conclusion: GJ-IF is the crucial component in the rapid antidepressant of GJ. Rapid and sustained antidepressant effects of GJ-IF were dependent on activating the PKA-CREB signaling pathway.
An Updated Meta-Analysis Based on the Preclinical Evidence of Mechanism of Aconitine-Induced Cardiotoxicity
Authors: Jiang Hong , Zhang Yating , Zhang Yi , Wang Xiaobo , Meng Xianli 
Published: June 2022

Background: Most Aconitum species in traditional Chinese medicine have the effect of dispelling wind, dehumidifying, warming the meridian, and relieving pain. Aconitine is the characteristic chemical component with the function of anti-inflammation, analgesic, and heart-strengthening effects. However, improper use will produce cardiotoxicity and neurotoxicity. Currently, the mechanisms of cardiotoxicity caused by aconitine are wheels within wheels without being fully disclosed. The systematic review and meta-analysis were therefore conducted to summarize the available evidence of myocardial toxicity caused by aconitine.Methods: We searched PubMed, Embase, Web of Science, National Knowledge Infrastructure, WANFANG, and VIP information database for relevant preclinical studies. All the data were analyzed by RevMan version 5.3.Results: Thirty-two studies met the final inclusion criteria, including both in vivo and in vitro study types. After aconitine treatment, the heart rate of animals was obviously abnormal, and the morphology and function of myocardial cells were significantly changed. Aconitine can induce changes in the electrophysiological activity of cardiac myocytes by regulating Na+, Ca2+, and K+ currents. Meanwhile, the mechanisms of cardiotoxicity of aconitine may be related to triggering mitochondrial dysfunction by inducing mitochondrial apoptosis and autophagy. It should not be ignored that the overactivation of NLRP3 inflammasome also exacerbates aconitine’s cardiotoxicity.Conclusion: The altered ion channels and mitochondrial function, as well as the signaling pathways interacting with NLRP3, may deserve further study for aconitine-induced cardiotoxicity.
RETRACTED: Intolerance of Uncertainty Scale-12: Psychometric Properties of This Construct Among Iranian Undergraduate Students
Authors: Vadivel Balachandran , Azadfar Zahra , Talib Mansor Abu , Mutlak Dhameer A. , Suksatan Wanich , Abbood Abbas Abd Ali , Sultan Mohammed Q. , Allen Kelly A. , Patra Indrajit , Hammid Ali Thaeer , Abdollahi Abbas , Chupradit Supat 
Published: June 2022

BackgroundUncertainty intolerance (IU), the tendency to think or react negatively toward uncertain events may have implication on individuals’ mental health and psychological wellbeing. The Intolerance of Uncertainty Scale-12 (IU-12) is commonly used across the globe to measure IU, however, its’ psychometric properties are yet to be evaluated in Iran with a Persian-speaking population. Therefore, the purpose of this research was to translate and validate the IU-12 among Iranian undergraduate students.Materials and MethodsThe multi-stage cluster random sampling was employed to recruit 410 Iranian undergraduate students (260 females) from the Azad University to complete the IU-12, the Depression Anxiety Stress Scale-2, and the Penn State Worry Questionnaire in a cross-sectional design. In this study, face validity, content validity, construct validity, and concurrent validity were measured and Construct Reliability (CR) and Cronbach’s alpha were used to measure reliability.ResultsThe impact score of the translated IU-12 indicated acceptable face validity (value of impact score was greater than 1.5). The value of Content Validity Index (CVI) and the value of Content Validity Ratio (CVR) were above 0.7 and 0.78, respectively. The values of CVI and CVR indicated the items had acceptable content validity and were deemed essential to the measure. The measurement model analysis showed the measure with two subscales had good fit indices (CMIN/df = 2.75, p &lt; 0.01, RMSEA = 0.07, TLI = 0.94, CFI = 0.95, GFI = 0.94). A Confirmatory Factor Analysis (CFA) indicated the scale was composed of the two subscales found in the English-version of the scale (prospective anxiety and inhibitory anxiety), and no items were removed from the scale. The values of CR (0.86) and Cronbach’s alphas (0.89) showed the measure had appropriate internal consistency.ConclusionThe findings support the psychometric properties of the Persian version of the IU-12. This scale could be used to reliably and accurately measure uncertainty intolerance among undergraduate students in Iran.
Laparoscopic Subtotal Gastrectomy and Sigmoidectomy Combined With Natural Orifice Specimen Extraction Surgery (NOSES) for Synchronous Gastric Cancer and Sigmoid Colon Cancer: A Case Report
Authors: Zhu Qingshun , Yu Lei , Zhu Guangxu , Jiao Xuguang , Li Bowen , Qu Jianjun 
Published: June 2022

BackgroundGastric cancer and colon cancer are rarely seen in clinic, but there are still related reports. For gastric cancer and simultaneous colon cancer, surgical resection is the main treatment. Traditional surgery requires an incision from xiphoid process to pubic symphysis. With the progress of minimally invasive technology, laparoscopic surgery is also used in the treatment of gastric cancer, but also in the abdominal incision to remove specimens and in vitro anastomosis of digestive tract. Taking specimens through the natural cavity as a new surgical method can not only reduce the abdominal incision, but also reduce the occurrence of wound-related complications. Here, we report a patient with gastric cancer and colon cancer who was treated in our hospital.Case SummaryWe report a series of patients with gastric cancer and colon cancer. upper abdominal pain was treated in our hospital for 6 months. electronic gastroscopy showed large irregular ulcers on the lesser curvature of the gastric antrum and biopsy showed poorly differentiated adenocarcinoma of the gastric antrum. The enhanced CT of abdomen and pelvis showed irregular thickening of gastric antrum wall, irregular thickening of sigmoid colon wall and no obvious enlarged lymph nodes around. Further electronic enteroscopy showed that the sigmoid colon showed cauliflower protuberance, the intestinal cavity was slightly narrow, the intestinal wall was stiff, and the biopsy pathology showed moderately differentiated adenocarcinoma of the sigmoid colon. No obvious abnormality was found in serological tumor indexes. We diagnosed gastric cancer with sigmoid colon cancer and the patient received Laparoscopic subtotal gastrectomy and sigmoidectomy combined with natural orifice specimen extraction surgery. At present, 12 months after operation, no clear tumor recurrence was found in the metastasis.ConclusionWe should improve the understanding of gastric cancer and sigmoid cancer and combine examination with pathology to avoid misdiagnosis as metastatic cancer. Laparoscopic subtotal gastrectomy should be performed for tumors with no serosa invasion, body mass index &lt;30 and tumor diameter &lt;6.5 cm. Sigmoidectomy combined with natural nostril sampling is feasible.
FOCUS ON TEACHING GRAMMAR IN CONTEXT
Authors: Бігунова Світозара Анатоліївна , Фрідріх Алла , Бігунов Дмитро 
Published: June 2022

The material presented in this article focuses on teaching grammar as the recent research recognizes the necessity of studying grammar and notes that learning is especially effective if grammar categories are included in meaningful communicative context. The paper is especially useful for secondary school English teachers as it identifies different options for focusing on grammar and integrating it into context, offers examples of learning activities for a specific option. The article also includes the description of the option, its theoretical and empirical basis and an example that illustrates the ways of its implementation in the classroom.
The paper highlights the basic principles for teaching grammar, namely E-Factor and A-Factor. E-Factor means Efficiency and, teaching any grammatical category, its components – economy, ease and efficacy – should be kept in mind. A-Factor means Appropriacy and contains a lot of factors, such as learners’ age, their hobbies and interests, learners’ previous level of knowledge, etc. Teachers should consider them all to succeed in grammar presentation. The authors offer multi-vector opportunities for learning grammar to encourage students to learn it understanding its necessity because mastering grammar is the basis of language proficiency.
ВИКОРИСТАННЯ ЕЛЕМЕНТІВ НАРОДОЗНАВСТВА НА УРОКАХ УКРАЇНСЬКОЇ МОВИ ЯК ОДНА З УМОВ ФОРМУВАННЯ КОМПЕТЕНТНОСТІ ВІЛЬНОГО ВОЛОДІННЯ ДЕРЖАВНОЮ МОВОЮ
Authors: Шуст Людмила , Гончарук Олена , Дацюк Вікторія 
Published: June 2022

Національне виховання школярів, зокрема в контексті розвитку компетентності вільного володіння державною мовою, є важливою ланкою у процесі розбудови незалежної України, духовного відродження і демократизації нашого суспільства. Найбільш придатними для роботи з народознавчим матеріалом є уроки української мови. Народознавчий матеріал у початковій школі досі використовується епізодично, без детально розробленої методики його опрацювання.
Ефективність освітнього процесу значною мірою залежить від рівня зацікавленості школярів. Використання художніх текстів, фольклору на уроках мови створюють можливості не лише для більш повного, глибокого оволодіння мовою, а й для розуміння естетичної природи літератури, її сутності як мистецтва слова.
Уведення в освітній процес народознавчих елементів дає змогу збагачувати уроки української мови та читання, розширювати словниковий запас здобувачів освіти, їхню стилістичну і загальну мовну культуру, грамотність. Важливо брати до уваги регіональні особливості національних традицій у вихованні учнів, за можливості проводити пошукову народознавчу роботу, під час якої діти могли б записувати фольклорні тексти безпосередньо від їх носіїв.
Засоби народознавства роблять урок більш цікавим, незвичайним, вносять у нього елементи гри, цікавості, прищеплюють інтерес до рідної мови, розширюють світогляд школяра, збільшують словниковий запас, розвивають мову, знайомлять дітей з українською історією та культурою.
ВИКОРИСТАННЯ КОМП’ЮТЕРНО-ІНФОРМАЦІЙНИХ ТЕХНОЛОГІЙ У ВИВЧЕННІ ІНОЗЕМНОЇ МОВИ
Authors: Коваленко Валентина , Пилипюк Лариса 
Published: June 2022

Питання застосування комп’ютерно-інформаційних технологій у навчанні, а саме у вивченні англійської мови досліджується у статті. Впровадження сучасних технологій, зокрема Інтернету, в навчальний процес створює широкі можливості для ефективної організації освітнього простору та розширення комунікативних можливостей навчання. Окреслено роль інтернет-ресурсів у навчальному процесі. Зазначено, що використання інтернет-ресурсів дозволяє організувати навчання англійської мови на основі принципів відкритості, адекватності та автентичності, а використання комп’ютерно-інформаційних технологій шляхом взаємодії викладача та студента забезпечує високий рівень персоналізації навчання на основі індивідуально-особистісного та технологічного підходів. Реалізується принцип автономності, що передбачає врахування особливостей студента, його індивідуальну освітню траєкторію, стимулювання самостійної пізнавальної діяльності та високої мотивації до вивчення мови. Визначено сутність поняття комп’ютерно-інформаційних технологій. Проаналізовано можливості їх використання у дистанційному навчанні іноземних мов і доступу до знань студентів з особливими потребами. Відзначено, що комп’ютерні програмні продукти можна розглядати як засіб об’єктивізації рівня вивчення іноземних мов.