bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global Characteristics of Cold Protons Around Midnight in the Magnetotail: Implication for Efficient Heating and Origin
Authors: Keika K. , Asami R. , Hoshino M. , Fuselier S. A. 
Published: July 2022

AbstractWe investigate plasma transport to and plasma heating in the plasma sheet in the noon‐midnight meridian, characterizing protons with temperature colder than the core plasma sheet protons (<700 eV). We extract the density and temperature of the cold protons from velocity distribution functions measured by the Hot Plasma Composition Analyzer instrument on board the Magnetospheric Multiscale spacecraft in the radial distance (r) of 6–25 Re, performing two‐component Maxwellian fits. We selected time intervals with no fast flow observed, to examine the characteristics of magnetotail plasma not directly affected by magnetic reconnection and associated phenomena. In the region of r > ∼10 Re, the two‐component populations are identified more frequently near the plasma sheet boundary than the central plasma sheet. The cold component density peaks near the boundary, in contrast to the hot components which display high density near the central plasma sheet. These characteristics suggest that cold protons are convected from the lobe by the open field lines and then heated and mixed with the plasma sheet hot plasma near the lobe‐plasma sheet boundary. The statistical features of the extracted cold components indicate that, in the tailward regions (r > ∼20 Re), temperature increases with decreasing vertical distance from the plasma sheet (represented by plasma β) in a similar trend to the hot components. In the near‐Earth plasma sheet (r < ∼15 Re), cold proton temperature is lower at higher‐β regions; the density decreases as increasing r. These features suggest that cold protons in the near‐Earth plasma sheet are of ionospheric origin, transported to the plasma sheet in the closed magnetic field configuration.
Contrasting Responses of Hailstorms to Anthropogenic Climate Change in Different Synoptic Weather Systems
Authors: Fan Jiwen , Zhang Yuwei , Wang Jingyu , Jeong Jong‐Hoon , Chen Xiaodong , Zhang Shixuan , Lin Yun , Feng Zhe , Adams‐Selin Rebecca 
Published: July 2022

AbstractHailstones and extreme precipitation generate substantial economic losses across the United States (US) and the globe. Their strong association with short‐lived, intense convective storms poses a great challenge in predicting their future changes. Here, we conducted model simulations at 1.2 km grid spacing for severe convective storms with large hail and heavy precipitation that occurred in two typical types of synoptic‐scale environments in spring seasons over the central US under both current and future climate conditions. We find that the responses of large hail (diameters >2.5 cm) to anthropogenic climate change (ACC) are markedly different between the hailstorms developed in the two types of synoptic‐scale environments, with over 110% increase in large hail occurrences for the frontal systems, whereas less than 30% increase for the Great Plains low‐level jet (GPLLJ) systems. This is explained by the larger increase in convective intensity and updraft width and a smaller increase in warm cloud depth in the frontal storms compared with the GPLLJ storms. Interestingly, the occurrences and intensity of heavy precipitation (rain rate >20 mm hr−1) in both types of systems are similarly sensitive to ACC (e.g., 40% and 33% increases in the occurrences for the frontal and GPLLJ systems, respectively). These results advance our knowledge of hail projection and have important implications for managing risks for future hail.
Proteinaceous Matter and Liquid Water in Fine Aerosols in Nanchang, Eastern China: Seasonal Variations, Sources, and Potential Connections
Authors: Xu Yu , Dong Xin‐Ni , Xiao Hua‐Yun , Zhou Jin‐Xiu , Wu Dai‐She 
Published: July 2022

AbstractThe transformation of aerosol proteinaceous matter (PrM) has potentially adverse environmental and health effects. The origins and atmospheric processes of PrM have obtained an increasing interest, but there are still large uncertainties. Although aerosol liquid water (ALW) has been found to be vital in numerous atmospheric processes, its impact on atmospheric degradation of PrM remains poorly understood. ALW, proteins, and low molecular weight PrM (LMW PrM, <10 kDa) were investigated in fine aerosols (PM2.5) collected in urban and suburban Nanchang (Eastern China) over a 1‐year period. The average concentrations of proteins and LMW PrM in the urban center were higher than those in the suburban area. Urban PrM tended to increase from cool season to warm season, which was opposite to the case of suburban PrM. These differences could be attributed to varied sources and atmospheric processes of PrM. Increase in ALW was prominent from the suburban site to the urban site, which was attributable to increased anthropogenic nitrate and sulfate. LMW PrM can be degraded from higher molecular weight PrM by ozone. Furthermore, correlation analysis between LMW PrM and ozone and ALW suggested that the enhanced ALW in the urban center facilitated the ozone‐induced LMW PrM release. The ALW‐related protein degradation was further supported by ozone exposure experiments with bovine serum albumin and PM2.5 samples under dry condition. Our findings suggest that ALW is a crucial promoter during PrM degradation by ozone, providing new insights into atmospheric transformation of proteins.
RacGAP1 promotes the malignant progression of cervical cancer by regulating AP-1 via miR-192 and p-JNK
Authors: Zhang Tianli , Wang Chunyan , Wang Kun , Liang Ying , Liu Ting , Feng Liping , Yang Xingsheng 
Published: July 2022

AbstractCervical cancer (CC) is the most frequently diagnosed genital tract cancer in females worldwide. Rac GTPase-activating protein 1 (RacGAP1) is one of the specific GTPase-activating proteins. As a novel tumor protooncogene, overexpression of RacGAP1 was related to the occurrence of various tumors, but its function in CC is still unclear. In this study, bioinformatics analyses showed that RacGAP1 might be a key candidate gene in the progression of CC. RacGAP1 was significantly overexpressed in CC tissues. High RacGAP1 expression was positively associated with poor prognosis. Downregulating RacGAP1 significantly inhibited the proliferation, migration, and invasion of CC cells, while overexpressing RacGAP1 had the opposite effects. Further research showed that miR-192, which plays as a tumor suppressor in CC, was identified as a downstream target of RacGAP1 in CC cells. miR-192 inhibition could partially rescue the decrease in cell proliferation, migration, and invasion caused by RacGAP1 downregulation. In opposite, miR-192 overexpression could decrease the promotion of malignant progression caused by RacGAP1 upregulation. Mechanism studies revealed that RacGAP1 could regulate the expression and phosphorylation of c-Jun, which was the component of AP-1, via miR-192 and p-JNK separately. These findings suggested that RacGAP1 promoted tumorigenicity, migration, and invasion of CC. Therefore, it represented a potential novel prognostic marker in CC and may probably be a therapeutic target.
Single cell sequencing identifies clonally expanded synovial CD4+ TPH cells expressing GPR56 in rheumatoid arthritis
Authors: Argyriou Alexandra , Wadsworth Marc H. , Lendvai Adrian , Christensen Stephen M. , Hensvold Aase H. , Gerstner Christina , van Vollenhoven Annika , Kravarik Kellie , Winkler Aaron , Malmström Vivianne , Chemin Karine 
Published: July 2022

AbstractRheumatoid arthritis (RA) is an autoimmune disease affecting synovial joints where different CD4+ T cell subsets may contribute to pathology. Here, we perform single cell sequencing on synovial CD4+ T cells from anti-citrullinated protein antibodies (ACPA)+ and ACPA- RA patients and identify two peripheral helper T cell (TPH) states and a cytotoxic CD4+ T cell subset. We show that the adhesion G-protein coupled receptor 56 (GPR56) delineates synovial CXCL13high TPH CD4+ T cells expressing LAG-3 and the tissue-resident memory receptors CXCR6 and CD69. In ACPA- SF, TPH cells display lower levels of GPR56 and LAG-3. Further, most expanded T cell clones in the joint are within CXCL13high TPH CD4+ T cells. Finally, RNA-velocity analyses suggest a common differentiation pathway between the two TPH clusters and effector CD4+ T cells. Our study provides comprehensive immunoprofiling of the synovial CD4+ T cell subsets in ACPA+ and ACPA- RA.
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery
Authors: Havugimana Pierre C. , Goel Raghuveera Kumar , Phanse Sadhna , Youssef Ahmed , Padhorny Dzmitry , Kotelnikov Sergei , Kozakov Dima , Emili Andrew 
Published: July 2022

AbstractCo-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order of magnitude faster than previous approaches. We apply mCF/MS to map the protein interaction landscape of non-transformed mammary epithelia versus breast cancer cells in parallel, revealing large-scale differences in protein-protein interactions and the relative abundance of associated macromolecules connected with cancer-related pathways and altered cellular processes. The integration of multiplexing capability within an optimized workflow renders mCF/MS as a powerful tool for systematically exploring physical interaction networks in a comparative manner.
Reconstitution of a telomeric replicon organized by CST
Authors: Zaug Arthur J. , Goodrich Karen J. , Song Jessica J. , Sullivan Ashley E. , Cech Thomas R. 
Published: July 2022

AbstractTelomeres, the natural ends of linear chromosomes, comprise repeat-sequence DNA and associated proteins1. Replication of telomeres allows continued proliferation of human stem cells and immortality of cancer cells2. This replication requires telomerase3 extension of the single-stranded DNA (ssDNA) of the telomeric G-strand ((TTAGGG)n); the synthesis of the complementary C-strand ((CCCTAA)n) is much less well characterized. The CST (CTC1–STN1–TEN1) protein complex, a DNA polymerase α-primase accessory factor4,5, is known to be required for telomere replication in vivo6–9, and the molecular analysis presented here reveals key features of its mechanism. We find that human CST uses its ssDNA-binding activity to specify the origins for telomeric C-strand synthesis by bound Polα-primase. CST-organized DNA polymerization can copy a telomeric DNA template that folds into G-quadruplex structures, but the challenges presented by this template probably contribute to telomere replication problems observed in vivo. Combining telomerase, a short telomeric ssDNA primer and CST–Polα–primase gives complete telomeric DNA replication, resulting in the same sort of ssDNA 3′ overhang found naturally on human telomeres. We conclude that the CST complex not only terminates telomerase extension10,11 and recruits Polα–primase to telomeric ssDNA4,12,13 but also orchestrates C-strand synthesis. Because replication of the telomere has features distinct from replication of the rest of the genome, targeting telomere-replication components including CST holds promise for cancer therapeutics.
European primary datasets of alien bacteria and viruses
Authors: Magliozzi Chiara , Artois Marc , Bertaccini Assunta , Candresse Thierry , Tsiamis Konstantinos , D’Amico Fabio , Deriu Ivan , Gervasini Eugenio , Cardoso Ana Cristina 
Published: July 2022

AbstractBacteria and viruses are a natural component of Earth biodiversity and play an essential role in biochemical and geological cycles. They may also pose problems outside their native range, where they can negatively impact on natural resources, wildlife, and human health. To address these challenges and develop sustainable conservation strategies, a thorough understanding of their invasion related- factors is needed: origin, country and year of introduction, and pathways dynamics. Yet, alien bacteria and viruses are underrepresented in invasion ecology studies, which limits our ability to quantify their impacts and address future introductions. This study provides primary datasets of alien bacteria and viruses of plants and animals present in the European environment. The datasets contain expert-revised data on 446 taxa and their invasion related- factors across terrestrial and aquatic environments. Taxa information are complemented with spatial occurrences. The datasets provide a basis for collaborative initiatives to improve the collection of alien bacteria and viruses’ data, and a starting point for data-driven conservation practices.
Experimental study on the influence of mental fatigue on risk decision-making of miners
Authors: Jia Aifang , Guo Xinyue , Tian Shuicheng 
Published: July 2022

AbstractMental fatigue increases risk-taking behavior. Using data collected between June 15 and August 6, 2020, this study investigates the impact of miners’ mental fatigue on risk decision-making to improve risk prevention and prediction abilities, and to reduce the occurrence of coal mine safety accidents. A total of 273 and 33 people participated in the preliminary and formal experiments, respectively. The participants, coal miners, visited a lab thrice to complete the pre-experiment, Balloon Analog Risk Task (BART), and Iowa Gambling Task (IGT). On the BART, mental fatigue displayed a significantly positive association with risk preference. On the IGT, as mental fatigue increased, net scores continuously decreased, while the frequency of making unfavorable decisions and the probability of taking risks increased. The BART value had no or weak correlations with the net score. Results suggest that mental fatigue leads to an increasing propensity to take risks. Therefore, regarding coal mine safety management, further attention is necessary concerning miners’ mental health, addressing mental fatigue, increasing rest time, and reducing night work. Furthermore, reasonable diet, improved working environments, and a positive attitude toward work should be promoted to reduce or eliminate mental fatigue and avoid decision-making errors that could cause accidents.
Maternal childbirth experience and time in labor: a population-based cohort study
Authors: Carlhäll Sara , Nelson Marie , Svenvik Maria , Axelsson Daniel , Blomberg Marie 
Published: July 2022

AbstractA negative childbirth experience may have long term negative effects on maternal health. New international guidelines allow a slower progress of labor in the early active phase. However, a longer time in labor may influence the childbirth experience. In this population-based cohort study including 26,429 women, who gave birth from January 2016 to March 2020, the association between duration of different phases of active labor and childbirth experience was studied. The women assessed their childbirth experience by visual analogue scale (VAS) score. Data was obtained from electronic medical records. The prevalence of negative childbirth experience (VAS 1–3) was 4.9%. A significant association between longer duration of all labor phases and a negative childbirth experience was found for primi- and multipara. The adjusted odds ratio (aOR (95%CI)) of negative childbirth experience and longer time in active labor (above the 90th percentile) in primipara was 2.39 (1.98–2.90) and in multipara 2.23 (1.78–2.79). In primi-and multipara with duration of labor ≥ 12 h or ≥ 6 h the aOR (95%CI) of negative childbirth experience were 2.22 (1.91–2.58) and 1.91 (1.59–2.26) respectively. It is of great importance to identify and optimize the clinical care of women with longer time in labor to reduce the risk of negative childbirth experience and associated adverse long-term effects.
Responsiveness of the Italian version of the Pediatric Quality of Life Multidimensional Fatigue Scale in adult inpatients with obesity
Authors: Smout Matthew F. , Manzoni Gian Mauro , Guerrini-Usubini Anna , Caroli Diana , De Col Alessandra , Castelnuovo Gianluca , Pietrabissa Giada , Molinari Enrico , Sartorio Alessandro 
Published: July 2022

AbstractThis study aimed to evaluate the responsiveness of the Italian version of the Paediatric Quality of Life Inventory Multidimensional Fatigue Scale (PedsQL-MFS) to changes in BMI, fatigue and depressive symptoms in adult inpatients with obesity. 198 adults (81% female, mean age = 44.7 years) with obesity completed the PedsQL-MFS, the Fatigue Severity Scale (FFS) and the Centre for Epidemiologic Studies Depression Scale (CESD) before and after completing a 3-week body weight reduction program. Internal responsiveness was measured via paired t-tests, standardized mean response (SMR) and Glass’s delta (d). Changes in FFS, CESD and BMI were used as anchors to categorize participants as “improved”, “unchanged” or “deteriorated”. External Responsiveness was assessed by comparing mean post-intervention PedsQL-MFS scores across change groups, adjusting for pre-intervention PedsQL-MFS scores and in area-under-curve (AUC) analysis. PedsQL-MFS Total, Sleep/Rest Fatigue and Cognitive Fatigue scores demonstrated significant reductions in response to an established body weight reduction program. Post-intervention PedsQL-MFS scale scores were lower among those who had improved on the CESD and FSS than among those whose CESD and FSS scores had not significantly changed. There was no difference in PedsQL-MFS scale scores according to whether participants had reduced their BMI by at least 5%. AUC analyses indicated that change in PedsQL-MFS scores was somewhat more predictive of improvement in CESD than FSS scores. The Italian version of the PedsQL-MFS demonstrated both internal and external responsiveness. It appeared more sensitive to improvement than deterioration in fatigue symptoms and its sensitivity to deterioration in depressive symptoms and weight loss could not be evaluated in the present study as there was no reliable deterioration in CESD scores and weight loss was modest. Future studies should include a control group to assess the sensitivity of the PedsQL-MFS more thoroughly.
Multi-objective optimization can balance trade-offs among boreal caribou, biodiversity, and climate change objectives when conservation hotspots do not overlap
Authors: Martin Amanda E. , Neave Erin , Kirby Patrick , Drever C. Ronnie , Johnson Cheryl A. 
Published: July 2022

AbstractThe biodiversity and climate change crises have led countries—including Canada—to commit to protect more land and inland waters and to stabilize greenhouse gas concentrations. Canada is also obligated to recover populations of at-risk species, including boreal caribou. Canada has the opportunity to expand its protected areas network to protect hotspots of high value for biodiversity and climate mitigation. However, co-occurrence of hotspots is rare. Here we ask: is it possible to expand the network to simultaneously protect areas important for boreal caribou, other species at risk, climate refugia, and carbon stores? We used linear programming to prioritize areas for protection based on these conservation objectives, and assessed how prioritization for multiple, competing objectives affected the outcome for each individual objective. Our multi-objective approach produced reasonably strong representation of value across objectives. Although trade-offs were required, the multi-objective outcome was almost always better than when we ignored one objective to maximize value for another, highlighting the risk of assuming that a plan based on one objective will also result in strong outcomes for others. Multi-objective optimization approaches could be used to plan for protected areas networks that address biodiversity and climate change objectives, even when hotspots do not co-occur.
Short-sighted decision-making by those not vaccinated against COVID-19
Authors: Halilova Julia G. , Fynes-Clinton Samuel , Green Leonard , Myerson Joel , Wu Jianhong , Ruggeri Kai , Addis Donna Rose , Rosenbaum R. Shayna 
Published: July 2022

AbstractWidespread vaccination is necessary to minimize or halt the effects of many infectious diseases, including COVID-19. Stagnating vaccine uptake can prolong pandemics, raising the question of how we might predict, prevent, and correct vaccine hesitancy and unwillingness. In a multinational sample (N = 4,452) recruited from 13 countries that varied in pandemic severity and vaccine uptake (July 2021), we examined whether short-sighted decision-making as exemplified by steep delay discounting—choosing smaller immediate rewards over larger delayed rewards—predicts COVID-19 vaccination status. Delay discounting was steeper in unvaccinated individuals and predicted vaccination status over and above demographics or mental health. The results suggest that delay discounting, a personal characteristic known to be modifiable through cognitive interventions, is a contributing cause of differences in vaccine compliance.
Predictive factors for massive hemorrhage in women with retained products of conception: a prospective study
Authors: Sonehara Reina , Nakamura Tomoko , Iwase Akira , Nishida Kazuki , Takikawa Sachiko , Murakami Mayuko , Yoshita Sayako , Muraoka Ayako , Miyake Natsuki , Nakanishi Natsuki , Osuka Satoko , Goto Maki , Kajiyama Hiroaki 
Published: July 2022

AbstractRetained products of conception (RPOC) is a common cause of postpartum bleeding, which may be life-threatening; however, no evidence-based guidelines exist to assist in evaluating the risk of massive hemorrhage in women with RPOC. In this prospective study, we aimed to evaluate the predictive factors for massive hemorrhage in women with RPOC. The primary and secondary endpoints were to validate the usefulness of power Doppler color scoring (PDCS) in evaluating hypervascularity and to identify other predictive factors (such as maximum RPOC diameter and serum βhCG and Hb level at first visit), respectively. Among the 51 women with RPOC included in this study, 16 (31.5%) experienced massive hemorrhage during follow-up. None of the women with PDCS 1 or 2 (18) experienced massive hemorrhage, whereas 16 (48.5%) women with PDCS 3 or 4 (33) did. Multiple logistic regression analysis showed that the odds ratio [95% confidence interval] (P value) for PDCS, assisted reproductive technology (ART), and low serum hemoglobin (Hb) levels were 22.39 [2.25 − 3087.92] (P = 0.004), 5.72 [1.28 − 33.29] (P = 0.022), and 4.24 [0.97 − 22.99] (P = 0.056), respectively. Further, the decision tree method identified PDCS, ART, and low serum Hb levels as potential predictive factors for massive hemorrhage. This study identified PDCS as useful predictor of massive hemorrhage in women with RPOC. With additional inclusion of factors such as ART and low serum Hb levels, the risk of massive hemorrhage may be effectively evaluated, leading to better management of women of reproductive age.