bluuebunny/crossref_metadata_2025_split huggingface dataset:
Informing plasmid compatibility with bacterial hosts using protein-protein interaction data
Authors: Downing Tim , Lee Min Jie , Archbold Conor , McDonnell Adam , Rahm Alexander 
Published: July 2022

AbstractThe compatibility of plasmids with new host cells is significant given their role in spreading antimicrobial resistance (AMR)1and virulence factor genes. Evaluating this usingin vitroscreening is laborious and can be informed by computational analyses of plasmid-host compatibility through rates of protein-protein interactions (PPIs) between plasmid and host cell proteins. We identified large excesses of such PPIs in eight important plasmids, including pOXA-48, using most known bacteria (n=4,363). 23 species had high rates of interactions with fourblaOXA-48-positive plasmids. We also identified 48 species with high interaction rates with plasmids common inEscherichia coli. We found a strong association between one plasmid and the fimbrial adhesin operonpil, which could enhance host cell adhesion in aqueous environments. An excess rate of PPIs could be a sign of host-plasmid compatibility, which is important for AMR control given that plasmids like pOXA-48 move between species with ease.HighlightsWe tested for protein interactions between key plasmids and 4,363 bacterial hosts23 host species had high rates of protein interactions with fourblaOXA-48 plasmidsPseudomonasspecies showed high rates of interactions with these plasmidsHost-plasmid protein-protein interaction rates may be associated with compatibility
Effects of Long-Term Alcohol Consumption on Behavior in the P301S (Line PS19) Tauopathy Mouse Model
Authors: Catavero Christina M. , Marsh Annelise E. , Downs Anthony M. , Teklezghi Adonay T. , Cohen Todd J. , McElligott Zoe A. 
Published: July 2022

AbstractAlcohol consumption and misuse remain prevalent public health issues with recent reports of increased heavy consumption in older populations in the United States. Several studies have identified alcohol consumption as a risk factor for developing multiple forms of dementia. The behavioral and psychological symptoms of dementia (BPSD), such as changes in affective behavior (e.g. anxiety), precede and coincide with cognitive decline. While many studies have characterized the intersection of alcohol use and affective behaviors, little is known regarding alcohol consumption and BPSD. This study characterizes the impact of long-term alcohol consumption on various behaviors in the P301S (line PS19) tauopathy mouse model. Male and female P301S and littermate control mice underwent two-bottle choice intermittent access to alcohol for sixteen weeks starting at 12 weeks of age. There were no significant differences in total ethanol consumption between wildtype and P301S mice of the same sex; however, drinking behavior differed among genotypes, and female mice of each genotype drank significantly more ethanol than males of each genotype. Following drinking studies, mice were run through a battery of behavioral tests during a period of forced abstinence, including approach/avoidance assays, social behavior tests, and memory and cognition tests. Across these tests we observed differences between groups due to genotype, alcohol history, and interactions between alcohol exposure and genotype. These differences were not always consistent between the sexes. In total, this study reveals significant alcohol-tauopathy interactions in subsequent behavior, which may have implications for understanding how alcohol may impact BPSD in conditions associated with tauopathy like Alzheimer’s disease and frontotemporal dementia.
Template-free prediction of a new monotopic membrane protein fold and oligomeric assembly by Alphafold2
Authors: Gulsevin Alican , Han Bing , Porta Jason C. , Mchaourab Hassane S. , Meiler Jens , Kenworthy Anne K. 
Published: July 2022

AbstractAlphaFold2 (AF2) has revolutionized the field of protein structural prediction. Here, we test its ability to predict the tertiary and quaternary structure of a previously undescribed scaffold with new folds and unusual architecture, the monotopic membrane protein caveolin-1 (CAV1). CAV1 assembles into a disc-shaped oligomer composed of 11 symmetrically arranged protomers, each assuming an identical new fold, and contains the largest parallel β-barrel known to exist in nature. Remarkably, AF2 predicts both the fold of the protomers and interfaces between them. It also assembles between 7 and 15 copies of CAV1 into disc-shaped complexes. However, the predicted multimers are energetically strained, especially the parallel β-barrel. These findings highlight the ability of AF2 to correctly predict new protein folds and oligomeric assemblies at a granular level while missing some elements of higher order complexes, thus positing a new direction for the continued development of deep learning protein structure prediction approaches.
Genetically Corrected RAG2-SCID Human Hematopoietic Stem Cells Restore V(D)J-Recombinase and Rescue Lymphoid Deficiency
Authors: Pavel-Dinu Mara , Gardner Cameron L. , Nakauchi Yusuke , Kawai Tomoki , Delmonte Ottavia M. , Palterer Boaz , Bosticardo Marita , Pala Francesca , Viel Sebastien , Malech Harry L. , Ghanim Hana Y. , Bode Nicole M. , Kurgan Gavin L. , Vakulskas Christopher A. , Sheikali Adam , Menezes Sherah T. , Chrobok Jade , Hernández González Elaine M. , Majeti Ravindra , Notarangelo Luigi D. , Porteus Matthew H. 
Published: July 2022

ABSTRACTRecombination-activating genes (RAG1 and RAG2) are critical in lymphoid cell development and function for initiating the V(D)J-recombination process to generate polyclonal lymphocytes with broad antigen-specificity. Clinical manifestations of defective RAG1/2 genes range from immune dysregulation to severe combined immunodeficiencies (SCID), causing life-threatening infections and death early in life in the absence of hematopoietic cell transplantation (HCT). Haploidentical HCT without myeloablative conditioning carries a high risk of graft failure and incomplete immune reconstitution. The RAG complex is only expressed during the G0-G1 phases of the cell cycle at the early stages of T and B cell development, underscoring that a direct gene correction would capture the precise temporal expression of the endogenous gene, is a promising therapeutic approach for RAG1/2-deficiencies. Here, we report a feasibility study using the CRISPR/Cas9-based “universal gene-correction” approach for the RAG2 locus in human hematopoietic stem/progenitor cells (HSPCs) in healthy donors and one RAG2-SCID patient. V(D)J recombinase activity was restored following gene correction of RAG2-SCID-derived HSPCs, resulting in the development of TCR αβ and γδ CD3+ cells and single-positive CD4+ and CD8+ lymphocytes. TCR repertoire analysis indicated a normal distribution of the CDR3 length and preserved usage of distal TRAV genes. We confirmed in vivo rescue of B-cell development, with normal IgM surface expression and a significant decrease in CD56bright NK cells. Together, we provide specificity, toxicity, and efficacy data supporting the development of a gene-correction therapy to benefit all RAG2-deficient patients.KEY POINTSHuman hematopoietic stem cells can be corrected to restore endogenous RAG2 gene expression while preserving durable engraftment potential.Gene-corrected RAG2 locus restores V(D)J recombination in RAG2-SCID patient stem cells, promoting T and B-cells’ receptor formation.
Structural basis for the enhanced infectivity and immune evasion of Omicron subvariants
Authors: Li Yaning , Shen Yaping , Zhang Yuanyuan , Yan Renhong 
Published: July 2022

AbstractThe Omicron variants of SARS-CoV-2 have recently become the globally dominant variants of concern in the COVID-19 pandemic. At least five major Omicron sub-lineages have been characterized: BA.1, BA.2, BA.3, BA.4 and BA.5. They all possess over 30 mutations on the Spike (S) protein. Here we report the cryo-EM structures of the trimeric S proteins from the five subvariants, of which BA.4 and BA.5 share the same mutations of S protein, each in complex with the surface receptor ACE2. All three receptor binding domains of S protein from BA.2 and BA.4/BA.5 are “up”, while the BA.1 S protein has two “up” and one “down”. The BA.3 S protein displays increased heterogeneity, with the majority in the all “up” RBD state. The differentially preferred conformations of the S protein are consistent with their varied transmissibilities. Analysis of the well defined S309 and S2K146 epitopes reveals the underlie immune evasion mechanism of Omicron subvariants.
Gender and mobility: enhancing the Egyptian working women’s travel pattern
Authors: Tawfik Radwa , Attia Sahar , Elbarmelgy Ingy Mohamed , Abdelaziz Tamer Mohamed 
Published: July 2022

PurposeWomen's travel pattern is different from those of men. Women who have both paid employment and unpaid care work have more complex travel patterns. However, land-use policies and urban mobility strategies in the Egyptian context do not consider these differences. This paper analyzes and discusses the travel patterns of the Egyptian working women with children. It examines the difference between men's and women's travel behavior in different income levels. The paper aims at determining the main factors that affect working women's travel patterns within the care economy framework and suggesting recommendations for enhancing women's travel patterns in Greater Cairo Region (GCR).Design/methodology/approachThe methodology relies on conducting a quantitative and qualitative analysis using questionnaires and interviews with working women and men from different social/economic levels in two different workplaces in GCR.FindingsThe results demonstrate that income level, workplace locations, schools locations, and schools typologies greatly affect working women's travel patterns in GCR.Originality/valueThe study findings will help urban planners and decision-makers to improve working women's mobility to make their daily trips shorter and more accessible to achieve equitable cities through understanding the conducted affecting factors and considering the suggested recommendations.
Obese customers’ fitness goal disclosure on social media: exploring weight-loss image sharing on emotions and healthy lifestyle aspirations
Authors: Singh Gurmeet , Sharma Shavneet 
Published: July 2022

Purpose
Obesity is today’s most neglected, yet blatantly visible, public health problem. This study aims to examine the role of social media and goal-directed behavior in motivating healthy lifestyle intentions for customers experiencing obesity. It investigates the distinct roles of self-conscious emotions (shame and pride) and weight-transformational posts shared by others on social media as moderators of these relationships.

Design/methodology/approach
The conceptual model uses the goal-directed behavior theory and social comparison theory, tested using data collected from 804 obese customers in Fiji through an experimental design.

Findings
Weight-loss transformation posts by others on social media, elicit distinct emotions for obese customers. Obese customers who felt guilt and shame due to shared weight-loss transformation posts showed a stronger association between goal disclosure and healthy lifestyle intention. In addition, the association between goal disclosure and healthy lifestyle intention is conditionally mediated by goal commitment, specifically for those obese customers that elicited guilt over shame due to shared weight-loss transformation posts by others on social media.

Research limitations/implications
Despite the adoption of an experimental design using a fictional stimulus being a commonly used method in marketing studies, external validity issues are likely. Also, this study examines obese customer behavior relating to Facebook. In addition, data collection for this study has been done from a single country perspective. Therefore, caution needs to be exercised when generalizing the findings of this study.

Practical implications
The findings assist businesses and marketers in the health and fitness industry to better leverage social media and goal-directed behavior and understand the emotions of obese customers to undertake data-driven precision marketing strategies.

Originality/value
The findings provide novel insights into goal disclosure and commitment, electronic word-of-mouth on social media platforms, self-conscious emotions and healthy lifestyle intentions for customers experiencing obesity.
Dynamic sentiment sensing of cities with social media data
Authors: Ye Guanghui , Peng Ze , Wei Jinyu , Hong Lingzi , Li SongYe , Wu Chuan 
Published: July 2022

PurposeA lot of people share their living or travelling experiences about cities by writing posts on social media. Such posts carry multi-dimensional information about the characteristics of cities from the public’s perspective. This paper aims at applying text mining technology to automatically extract city images, which are known as how observers perceive the status of the city, from these social media texts.Design/methodology/approachThis paper proposes a data processing pipeline for automatic city image extraction and applies sentiment analysis, timing analysis and contrastive analysis in a case study on Wuhan, a central China megacity. Specifically, the city image constructed with social media text and the expected policy outcomes by the government are compared.FindingsResults reveal gaps between the public’s impression and the strategic goals of the government in traffic and environment.Originality/valueThis study contributes a novel approach to assess government performance by complementary data from social media. This case study implies the value of social media-based city image in the identification of gaps for the optimization of government performance.
Situating futures literacies in the Colombian educational system: a decolonizing theoretical model
Authors: Anderson Carl Edlund , David Rosa Dene 
Published: July 2022

Purpose
This paper aims to present a theoretical model for restructuring Colombia’s educational initiatives in response to current socioeconomic needs. More equitable and decolonized education could help learners decouple their capacities to imagine the future from colonialized paradigms, thereby opening spaces for more active engagement in their own futures.

Design/methodology/approach
The authors take a critical, postmodern approach focused on empowering people to transcend constraints from a colonial past and recognizing that the purpose of knowledge, although reflecting power and social relationships, is to help people improve society. Notions of situated and futures literacies nourish an approach toward a decolonized and glocalized educational model.

Findings
The current Colombian educational system tends to favor a single focus – local, national or international – at the expense of the others. The authors argue that educational policy and planning should account for three realms of knowledge: locally situated literacies, nationally situated literacies and globally situated literacies.

Originality/value
Deconstructing obsolete and colonized methodologies could not only help prepare Colombian learners for active engagement both within and beyond their modern-day borders but could also help transform other educational systems originally designed to support societies and economies that no longer exist, including those of the Global North.
Determinants of Iberian companies’ performance: corporate boards and the nonlinearity of gender diversity
Authors: Proença Catarina , Neves Maria Elisabete 
Published: July 2022

Purpose
This paper aims to analyze the performance determinants of listed companies in the Iberian Peninsula, focusing on the analysis of the effect of gender diversity and the structure of the board of directors.

Design/methodology/approach
To achieve this aim, the authors analyzed 97 listed companies, of which 23 are Portuguese and 74 are Spanish, between 2015 and 2019. The authors use Arellano and Bond’s (1991) generalized method of moments system model to test the hypotheses.

Findings
The results show an important impact of corporate governance variables on corporate performance. Specifically, board size, average director age and board academic qualifications are crucial to explaining profitability and market value. Moreover, the authors identified a nonlinear relationship between gender diversity and profitability and market value levels due to critical mass theory and quotas that enhance more social justice. The authors concluded that the corporate performance determinants differ depending on the performance measures.

Originality/value
To the best of the authors’ knowledge, this study is the first to analyze the nonlinear effect of gender diversity and board structure (size, educational qualifications and average director age) on the performance of Iberian listed companies as a single market.
Why customer mistreatment undermines hospitality employees’ performance: the moderating role of job crafting
Authors: Lu Wenzhu , Wu Haibo , Liu Shanshi , Guo Zisheng , He Xiongtao 
Published: July 2022

PurposeBased on the person-environment (P-E) fit theory, this study aims to explore the effect of customer mistreatment on the reduced service performance of hospitality employees mediated by person-job (P-J) fit perceptions and moderated by job crafting.Design/methodology/approachThe authors tested this study’s hypotheses with a nine-day diary study involving 83 service employees located in Lanzhou, China; a total of 548 daily surveys were completed. The authors used multilevel structural equation modeling to analyze the data.FindingsEmployees who experienced daily customer mistreatment suffered diminished P-J fit perceptions, leading to lower levels of service performance the next day. In addition, job crafting significantly buffered the impact of customer mistreatment on P-J fit perceptions and the indirect impact of customer mistreatment on service performance through P-J fit perceptions.Practical implicationsGiven the damaging effect that customer mistreatment has on service performance, where employees’ P-J fit perceptions are impaired, hotel managers should implement service competence improvement training programs and managerial preventions to reduce the possibility of customer mistreatment behavior. The moderating role of job crafting behavior suggests that managers should offer supportive practices (i.e. job autonomy) to encourage job crafting behaviors among employees.Originality/valueThis study reveals that individuals’ P-J fit perceptions can explain the damaging impacts of customer mistreatment on service performance, a finding that contributes valuable information to the literature on customer mistreatment and P-E fit. Second, this study also tests the impact of individuals’ job crafting behaviors in terms of mitigating the negative effect of customer mistreatment. Finally, this study’s findings broaden the scope of predictors of P-J fit perceptions by revealing that customer mistreatment can pose a threat to hospitality employees’ P-J fit perceptions.
Net-zero economy research in the field of supply chain management: a systematic literature review and future research agenda
Authors: Mishra Ruchi , Singh Rajesh , Govindan Kannan 
Published: July 2022

PurposeThe purpose of this study is to systematically review the state-of-art literature on the net-zero economy in the field of supply chain management.Design/methodology/approachA systematic literature review of 79 articles published from 2009 to 2021 has been conducted to minimise the researchers' bias and maximise the reliability and replicability of the study.FindingsThe thematic analysis reveals that studies in the field of net-zero economy have mostly been done on decarbonisation in the supply chain, emission control and life cycle analysis and environmental and energy management. The findings highlight the strong positive association between digitalisation, circular economy and resources optimization practices with net-zero economy goals. The study also addresses the challenges linked with the net-zero economy at the firm and country levels.Research limitations/implicationsPractitioners in companies and academics might find this review valuable as this study reviews, classifies and analyses the studies, outlines the evolution of literature and offers directions for future studies using the theory, methodology and context (TMC) framework.Originality/valueThis is the first study that uses a structured approach to analyse studies done in the net-zero field by assessing publications from 2009 to 2021.
Clarifying the conceptual map of VUCA: a systematic review
Authors: Taskan Burcu , Junça-Silva Ana , Caetano António 
Published: July 2022

Purpose
Over the past few decades, the environment for organisations has been frequently described using the acronym VUCA: a volatile, uncertain, complex and ambiguous environment. In spite of the popularity of this acronym, it is not unusual to find some overlap concerning the meaning of those terms, as well as poor definitions of each in the literature. Consequently, the main purpose of this paper was to conduct a systematic literature review to obtain a conceptual map of the components of VUCA and their relationships and to highlight some avenues for future research.

Design/methodology/approach
The authors conducted a systematic review of various databases between 1999 and 2021. A total of 833 papers were identified and 26 of them met the inclusion criteria for the current study.

Findings
The subsequent analysis revealed several overlaps and relationships between the four terms. Based on this analysis, the authors propose a conceptual map that could serve as a basis for future research and practice.

Research limitations/implications
Because of the exploratory nature of the study and the scarce number of empirical studies, the impact that the use of the VUCA framework has had on businesses could not be addressed.

Originality/value
By clarifying the different components of VUCA and specifying the relationships between them with a comprehensive conceptual map, this paper may contribute to more rigorous empirical research, as well as help managers and executives more effectively deal with turbulent environments.
Testing an instrument to assess the perception of climate change policies in universities: the case of Salamanca University
Authors: Ferrari Enzo , Reyes-Carrasco Paula Mariel , Barrón Ruíz Angela , Ruíz Camilo 
Published: July 2022

Purpose
This study aims to introduce a new instrument to assess the perception of the university community after the Climate Emergency Declaration (CED) and its application at the Universidad de Salamanca (USAL) in Spain. This CED includes a comprehensive program to reduce the carbon footprint and the introduction of the education for sustainable development in the curriculum. This study aims to understand the gap between perception and reality in the program's implementation and verify whether the student organization's initial push translates into higher approval ratings.

Design/methodology/approach
The authors conducted a cross-sectional analytical study. In this research, the authors used a sample of 731 people from the USAL community (67% students, 20% academics and the rest administrative and service staff).

Findings
The findings revealed that PhD students are the group with the highest perception of the policies implemented by the university to fight climate change, even higher than academics. In addition, the perception of the food, energy and sustainable mobility aspects of the program are good predictors: of the knowledge of the indicators and of the policy dimensions within the CED.

Originality/value
The university community perception survey for a CED process provides a gateway into the gaps between perception, expectations and reality. Moreover, it is helpful to engage its members in action plans to fight climate change and its impacts.