bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interventions targeting psychological well-being for motor neuron disease carers: A systematic review
Authors: Cafarella Paul , Effing Tanja , Chur-Hansen Anna 
Published: March 2022

AbstractObjectivesThis systematic review considers interventions designed to improve the psychological well-being (PWB) of carers of people with motor neuron disease (MND) using quantitative, qualitative, or mixed-methods studies, and aimed to (1) summarize current research, (2) assess the quality of evidence, and (3) evaluate the effectiveness of interventions.MethodMixed-methods systematic review (MMSR) was conducted based on Joanna Briggs Institute methodology for quantitative, qualitative, and mixed-methods reviews and Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.ResultsThirteen papers met the inclusion criteria, including 12 studies (six mixed-methods, four quantitative, and two qualitative). Four studies described randomized controlled trials, seven detailed uncontrolled longitudinal studies with a single treatment group and a pre-post design, and one was an observational survey. Critical appraisal of the studies revealed a wide range of weaknesses in the quantitative and/or qualitative methodologies. Due to the heterogeneity of interventions, outcomes, and measurements, a narrative and convergent approach to data synthesis was employed. While a minority of studies demonstrated some benefits to hedonic and eudaimonic aspects of PWB, the interpretability of these data was limited by methodological problems.Significance of resultsThis MMSR highlighted a paucity of quality research regarding interventions for the PWB of MND carers. Although some benefits to PWB were demonstrated, most studies suffered from substantial methodological problems, rendering the overall evidence base low. High-quality and carefully designed studies are a priority to enable effective development and testing of much-needed interventions targeting the PWB for MND carers.
Strategies to improve the control of glyphosate-resistant horseweed (Erigeron canadensis) with glufosinate applied preplant to soybean
Authors: Dilliott Meghan , Soltani Nader , Hooker David C. , Robinson Darren E. , Sikkema Peter H. 
Published: March 2022

AbstractThe objectives of this study were to determine if the level and consistency of glyphosate-resistant (GR) horseweed control prior to soybean planting can be improved by (i) adding halauxifen-methyl, 2,4-D ester, saflufenacil, metribuzin, or dicamba to glufosinate, (ii) increasing the rate of glufosinate from 500 to 1,000 g ai ha–1, and (iii) adding 28% urea ammonium nitrate (UAN) as the carrier solution. During a 2-yr period (2020–2021), four field trials were conducted on commercial farms located in southwestern Ontario, Canada, with confirmed GR horseweed. Glufosinate controlled GR horseweed 65%, 66%, and 63% at 2, 4, and 8 wk after application (WAA), respectively, and reduced density and biomass 46% and 33% at 8 WAA, respectively. There was no improvement in GR horseweed control from the addition of halauxifen-methyl, 2,4-D ester or saflufenacil to glufosinate and no decrease in density and biomass, with the exception that the addition of saflufenacil to glufosinate reduced density 30% compared to glufosinate alone. The addition of metribuzin to glufosinate improved GR horseweed control by 22%, 22%, and 28% at 2, 4, and 8 WAA, respectively, and further reduced density and biomass 50% and 47%, respectively, at 8 WAA, respectively. The addition of dicamba to glufosinate improved GR horseweed control by 19%, 26%, and 30% at 2, 4, and 8 WAA, respectively, and further reduced density and biomass 54% and 60%, respectively, at 8 WAA. There was no improvement in GR horseweed control by increasing the rate of glufosinate from 500 to 1,000 g ai ha–1 or when using 28% UAN as the carrier solution. The addition of all herbicides to glufosinate, increasing the rate of glufosinate, or using 28% UAN as the carrier solution improved the consistency of GR horseweed control.
Target-site cross-resistance to ALS inhibitors in johnsongrass originating from Greek cornfields
Authors: Papapanagiotou Aristeidis P. , Loukovitis Dimitrios , Ntoanidou Symela , Eleftherohorinos Ilias G. 
Published: March 2022

AbstractFive johnsongrass populations collected from corn grown in northern Greece were studied to elucidate the levels and mechanisms of resistance to acetolactate synthase (ALS)- and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Whole-plant response assays indicated that two populations were highly cross-resistant to all ALS inhibitors tested (foramsulfuron, nicosulfuron, rimsulfuron, and imazamox) but were effectively controlled by the recommended rate of the ACCase-inhibiting herbicides propaquizafop and clethodim. TheALSgene sequence revealed a point mutation that resulted in the substitution of Trp574 by Leu in the ALS enzyme, suggesting that the resistance mechanism is target-site mediated. These findings highlight a serious threat against the sustainable use of the ALS-inhibiting herbicides in controlling johnsongrass and other grass weeds in cornfields, suggesting rotational use of herbicides with different modes of action, along with the use of nonchemical methods, for viable Johnsongrass management.
Potential Impact of Spring Thermal Forcing Over the Tibetan Plateau on the Following Winter El Niño–Southern Oscillation
Authors: Yu Wei , Liu Yimin , Xu Lianlian , Wu Guoxiong , Yang Song , Chen Dake , Yang Xiu‐Qun , Hu Chundi , He Bian 
Published: March 2022

AbstractUsing observational analysis and numerical experiments, we identify that the dipole mode of spring surface wind speed (SWS) over the Tibetan Plateau (TP) could act as a trigger for subsequent winter El Niño–Southern Oscillation events. During the positive phase of spring SWS dipole mode (south‐positive and north‐negative), a self‐sustaining “negative sensible heating–baroclinic structure” prevails over the western TP, which is characterized by negative surface sensible heating anomalies, anomalous low‐level anticyclones, and mid–high‐level cyclones. The “negative sensible heating–baroclinic structure” stimulates the surface westerly wind anomalies over the tropical western Pacific in May through two pathways, favoring the occurrence of subsequent El Niño events. One is through weakening the zonal monsoon circulation over the tropical Indian Ocean and the Walker circulation over the tropical western Pacific. The other is modulating the air–sea interaction over the North Pacific through triggering Rossby waves. The negative SWS dipole mode tends to induce La Niña events.
Transport of Phosphorus in the Hyporheic Zone
Authors: Jin Guangqiu , Chen Hexiang , Zhang Zhongtian , Jiang Qihao , Liu Zhenyu , Tang Hongwu 
Published: March 2022

AbstractPhosphorus is not only an essential nutrient for organisms but also a major contributor to the eutrophication of fluvial ecosystems. Previous studies have focused on the flux of phosphorus at the water‐sediment interface, and very little is known about the transport and distribution of phosphorus in bedforms. In this study, a series of flume experiments and numerical simulations were conducted to investigate phosphorus dynamics in the hyporheic zone. The results show that the phosphorus concentration in the overlying water decreases faster than that of conservative solutes because of sediment adsorption. It is also found that the hyporheic exchange flux of phosphorus is more sensitive to the maximum adsorption capacity than to the equilibrium isotherm distribution coefficient, and the penetration depth is inhibited at higher maximum adsorption capacity. The mass proportion of phosphorus is decreased by 19.5% in the pore water but increased by 95.9% in the sediment with the increase of the maximum adsorption capacity from 0.5 to 1. These findings provide a better understanding of the transport and distribution of phosphorus in the riverbed and the contribution of transient flows such as flood to phosphorus dynamics in the hyporheic zone.
Characterizing cerebral metabolite profiles in anorexia and bulimia nervosa and their associations with habitual behavior
Authors: Westwater Margaret L. , Murley Alexander G. , Diederen Kelly M. J. , Carpenter T. Adrian , Ziauddeen Hisham , Fletcher Paul C. 
Published: March 2022

AbstractAnorexia nervosa (AN) and bulimia nervosa (BN) are associated with altered brain structure and function, as well as increased habitual behavior. This neurobehavioral profile may implicate neurochemical changes in the pathogenesis of these illnesses. Altered glutamate, myo-inositol and N-acetyl aspartate (NAA) concentrations are reported in restrictive AN, yet whether these extend to binge-eating disorders, or relate to habitual traits in affected individuals, remains unknown. We therefore used single-voxel proton magnetic resonance spectroscopy to measure glutamate, myo-inositol, and NAA in the right inferior lateral prefrontal cortex and the right occipital cortex of 85 women [n = 22 AN (binge-eating/purging subtype; AN-BP), n = 33 BN, n = 30 controls]. To index habitual behavior, participants performed an instrumental learning task and completed the Creature of Habit Scale. Women with AN-BP, but not BN, had reduced myo-inositol and NAA concentrations relative to controls in both regions. Although patient groups had intact instrumental learning task performance, both groups reported increased routine behaviors compared to controls, and automaticity was related to reduced prefrontal glutamate and NAA participants with AN-BP. Our findings extend previous reports of reduced myo-inositol and NAA levels in restrictive AN to AN-BP, which may reflect disrupted axonal-glial signaling. Although we found inconsistent support for increased habitual behavior in AN-BP and BN, we identified preliminary associations between prefrontal metabolites and automaticity in AN-BP. These results provide further evidence of unique neurobiological profiles across binge-eating disorders.
A systematic review of microbial markers for risk prediction of colorectal neoplasia
Authors: Yu Lili , Zhao Gang , Wang Lijuan , Zhou Xuan , Sun Jing , Li Xinxuan , Zhu Yingshuang , He Yazhou , Kofonikolas Kleovoulos , Bogaert Debby , Dunlop Malcolm , Zhu Yimin , Theodoratou Evropi , Li Xue 
Published: March 2022

AbstractBackgroundSubstantial evidence indicates that dysbiosis of the gut microbial community is associated with colorectal neoplasia. This review aims to systematically summarise the microbial markers associated with colorectal neoplasia and to assess their predictive performance.MethodsA comprehensive literature search of MEDLINE and EMBASE databases was performed to identify eligible studies. Observational studies exploring the associations between microbial biomarkers and colorectal neoplasia were included. We also included prediction studies that constructed models using microbial markers to predict CRC and adenomas. Risk of bias for included observational and prediction studies was assessed.ResultsForty-five studies were included to assess the associations between microbial markers and colorectal neoplasia. Nine faecal microbiotas (i.e.,Fusobacterium, Enterococcus, Porphyromonas, Salmonella, Pseudomonas, Peptostreptococcus,Actinomyces, BifidobacteriumandRoseburia), two oral pathogens (i.e.,Treponema denticolaandPrevotella intermedia) and serum antibody levels response toStreptococcus gallolyticus subspecies gallolyticuswere found to be consistently associated with colorectal neoplasia. Thirty studies reported prediction models using microbial markers, and 83.3% of these models had acceptable-to-good discrimination (AUROC > 0.75). The results of predictive performance were promising, but most of the studies were limited to small number of cases (range: 9–485 cases) and lack of independent external validation (76.7%).ConclusionsThis review provides insight into the evidence supporting the association between different types of microbial species and their predictive value for colorectal neoplasia. Prediction models developed from case-control studies require further external validation in high-quality prospective studies. Further studies should assess the feasibility and impact of incorporating microbial biomarkers in CRC screening programme.
A microphysiological model of human trophoblast invasion during implantation
Authors: Park Ju Young , Mani Sneha , Clair Geremy , Olson Heather M. , Paurus Vanessa L. , Ansong Charles K. , Blundell Cassidy , Young Rachel , Kanter Jessica , Gordon Scott , Yi Alex Y. , Mainigi Monica , Huh Dan Dongeun 
Published: March 2022

AbstractSuccessful establishment of pregnancy requires adhesion of an embryo to the endometrium and subsequent invasion into the maternal tissue. Abnormalities in this critical process of implantation and placentation lead to many pregnancy complications. Here we present a microenigneered system to model a complex sequence of orchestrated multicellular events that plays an essential role in early pregnancy. Our implantation-on-a-chip is capable of reconstructing the three-dimensional structural organization of the maternal-fetal interface to model the invasion of specialized fetal extravillous trophoblasts into the maternal uterus. Using primary human cells isolated from clinical specimens, we demonstrate in vivo-like directional migration of extravillous trophoblasts towards a microengineered maternal vessel and their interactions with the endothelium necessary for vascular remodeling. Through parametric variation of the cellular microenvironment and proteomic analysis of microengineered tissues, we show the important role of decidualized stromal cells as a regulator of extravillous trophoblast migration. Furthermore, our study reveals previously unknown effects of pre-implantation maternal immune cells on extravillous trophoblast invasion. This work represents a significant advance in our ability to model early human pregnancy, and may enable the development of advanced in vitro platforms for basic and clinical research of human reproduction.
Myosin VI regulates the spatial organisation of mammalian transcription initiation
Authors: Hari-Gupta Yukti , Fili Natalia , dos Santos Ália , Cook Alexander W. , Gough Rosemarie E. , Reed Hannah C. W. , Wang Lin , Aaron Jesse , Venit Tomas , Wait Eric , Grosse-Berkenbusch Andreas , Gebhardt J. Christof M. , Percipalle Piergiorgio , Chew Teng-Leong , Martin-Fernandez Marisa , Toseland Christopher P. 
Published: March 2022

AbstractDuring transcription, RNA Polymerase II (RNAPII) is spatially organised within the nucleus into clusters that correlate with transcription activity. While this is a hallmark of genome regulation in mammalian cells, the mechanisms concerning the assembly, organisation and stability remain unknown. Here, we have used combination of single molecule imaging and genomic approaches to explore the role of nuclear myosin VI (MVI) in the nanoscale organisation of RNAPII. We reveal that MVI in the nucleus acts as the molecular anchor that holds RNAPII in high density clusters. Perturbation of MVI leads to the disruption of RNAPII localisation, chromatin organisation and subsequently a decrease in gene expression. Overall, we uncover the fundamental role of MVI in the spatial regulation of gene expression.
Reversible writing/deleting of magnetic skyrmions through hydrogen adsorption/desorption
Authors: Chen Gong , Ophus Colin , Quintana Alberto , Kwon Heeyoung , Won Changyeon , Ding Haifeng , Wu Yizheng , Schmid Andreas K. , Liu Kai 
Published: March 2022

AbstractMagnetic skyrmions are topologically nontrivial spin textures with envisioned applications in energy-efficient magnetic information storage. Toggling the presence of magnetic skyrmions via writing/deleting processes is essential for spintronics applications, which usually require the application of a magnetic field, a gate voltage or an electric current. Here we demonstrate the reversible field-free writing/deleting of skyrmions at room temperature, via hydrogen chemisorption/desorption on the surface of Ni and Co films. Supported by Monte-Carlo simulations, the skyrmion creation/annihilation is attributed to the hydrogen-induced magnetic anisotropy change on ferromagnetic surfaces. We also demonstrate the role of hydrogen and oxygen on magnetic anisotropy and skyrmion deletion on other magnetic surfaces. Our results open up new possibilities for designing skyrmionic and magneto-ionic devices.
Choice of vector and surgical approach enables efficient cochlear gene transfer in nonhuman primate
Authors: Andres-Mateos Eva , Landegger Lukas D. , Unzu Carmen , Phillips Jean , Lin Brian M. , Dewyer Nicholas A. , Sanmiguel Julio , Nicolaou Fotini , Valero Michelle D. , Bourdeu Kathrin I. , Sewell William F. , Beiler Rudolph J. , McKenna Michael J. , Stankovic Konstantina M. , Vandenberghe Luk H. 
Published: March 2022

AbstractInner ear gene therapy using adeno-associated viral vectors (AAV) promises to alleviate hearing and balance disorders. We previously established the benefits of Anc80L65 in targeting inner and outer hair cells in newborn mice. To accelerate translation to humans, we now report the feasibility and efficiency of the surgical approach and vector delivery in a nonhuman primate model. Five rhesus macaques were injected with AAV1 or Anc80L65 expressing eGFP using a transmastoid posterior tympanotomy approach to access the round window membrane after making a small fenestra in the oval window. The procedure was well tolerated. All but one animal showed cochlear eGFP expression 7–14 days following injection. Anc80L65 in 2 animals transduced up to 90% of apical inner hair cells; AAV1 was markedly less efficient at equal dose. Transduction for both vectors declined from apex to base. These data motivate future translational studies to evaluate gene therapy for human hearing disorders.
Intrinsically disordered intracellular domains control key features of the mechanically-gated ion channel PIEZO2
Authors: Verkest Clement , Schaefer Irina , Nees Timo A. , Wang Na , Jegelka Juri M. , Taberner Francisco J. , Lechner Stefan G. 
Published: March 2022

AbstractA central question in mechanobiology is how mechanical forces acting in or on cells are transmitted to mechanically-gated PIEZO channels that convert these forces into biochemical signals. Here we examined the role of the intracellular domains of PIEZO2, which account for 25% of the channel, and demonstrate that these domains fine-tune properties such as poking and stretch-sensitivity, velocity coding and single channel conductance. Moreover, we show that the intrinsically disordered linker between the transmembrane helices twelve and thirteen (IDR5) is required for the activation of PIEZO2 by cytoskeleton-transmitted forces. The deletion of IDR5 abolishes PIEZO2-mediated inhibition of neurite outgrowth, while it only partially affected its sensitivity to cell indentation and does not alter its stretch sensitivity. Thus, we propose that PIEZO2 is a polymodal mechanosensor that detects different types of mechanical stimuli via different force transmission pathways, which highlights the importance of utilizing multiple complementary assays when investigating PIEZO function.
A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity
Authors: Eslami-Mossallam Behrouz , Klein Misha , Smagt Constantijn V. D. , Sanden Koen V. D. , Jones Stephen K. , Hawkins John A. , Finkelstein Ilya J. , Depken Martin 
Published: March 2022

AbstractThe S. pyogenes (Sp) Cas9 endonuclease is an important gene-editing tool. SpCas9 is directed to target sites based on complementarity to a complexed single-guide RNA (sgRNA). However, SpCas9-sgRNA also binds and cleaves genomic off-targets with only partial complementarity. To date, we lack the ability to predict cleavage and binding activity quantitatively, and rely on binary classification schemes to identify strong off-targets. We report a quantitative kinetic model that captures the SpCas9-mediated strand-replacement reaction in free-energy terms. The model predicts binding and cleavage activity as a function of time, target, and experimental conditions. Trained and validated on high-throughput bulk-biochemical data, our model predicts the intermediate R-loop state recently observed in single-molecule experiments, as well as the associated conversion rates. Finally, we show that our quantitative activity predictor can be reduced to a binary off-target classifier that outperforms the established state-of-the-art. Our approach is extensible, and can characterize any CRISPR-Cas nuclease – benchmarking natural and future high-fidelity variants against SpCas9; elucidating determinants of CRISPR fidelity; and revealing pathways to increased specificity and efficiency in engineered systems.
Pressure-induced liquid-liquid transition in a family of ionic materials
Authors: Wojnarowska Zaneta , Cheng Shinian , Yao Beibei , Swadzba-Kwasny Malgorzata , McLaughlin Shannon , McGrogan Anne , Delavoux Yoan , Paluch Marian 
Published: March 2022

AbstractLiquid−liquid transition (LLT) between two disordered phases of single-component material remains one of the most intriguing physical phenomena. Here, we report a first-order LLT in a series of ionic liquids containing trihexyl(tetradecyl)phosphonium cation [P666,14]+ and anions of different sizes and shapes, providing an insight into the structure-property relationships governing LLT. In addition to calorimetric proof of LLT, we report that ion dynamics exhibit anomalous behavior during the LLT, i.e., the conductivity relaxation times (τσ) are dramatically elongated, and their distribution becomes broader. This peculiar behavior is induced by isobaric cooling and isothermal compression, with the τσ(TLL,PLL) constant for a given system. The latter observation proves that LLT, in analogy to liquid-glass transition, has an isochronal character. Finally, the magnitude of discontinuity in a specific volume at LLT was estimated using the Clausius-Clapeyron equation.
Battery technology and recycling alone will not save the electric mobility transition from future cobalt shortages
Authors: Zeng Anqi , Chen Wu , Rasmussen Kasper Dalgas , Zhu Xuehong , Lundhaug Maren , Müller Daniel B. , Tan Juan , Keiding Jakob K. , Liu Litao , Dai Tao , Wang Anjian , Liu Gang 
Published: March 2022

AbstractIn recent years, increasing attention has been given to the potential supply risks of critical battery materials, such as cobalt, for electric mobility transitions. While battery technology and recycling advancement are two widely acknowledged strategies for addressing such supply risks, the extent to which they will relieve global and regional cobalt demand–supply imbalance remains poorly understood. Here, we address this gap by simulating historical (1998-2019) and future (2020-2050) global cobalt cycles covering both traditional and emerging end uses with regional resolution (China, the U.S., Japan, the EU, and the rest of the world). We show that cobalt-free batteries and recycling progress can indeed significantly alleviate long-term cobalt supply risks. However, the cobalt supply shortage appears inevitable in the short- to medium-term (during 2028-2033), even under the most technologically optimistic scenario. Our results reveal varying cobalt supply security levels by region and indicate the urgency of boosting primary cobalt supply to ensure global e-mobility ambitions.