bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircCDK17 knockdown inhibits tumor progression and cell glycolysis by downregulaing YWHAZ expression through sponging miR-1294 in cervical cancer
Authors: Chen Rui , Liang Fei , Yan Jun , Wang Yu 
Published: February 2022

AbstractBackgroundCervical cancer (CC) is the fourth aggressive tumor affecting women worldwide. Circular RNA (circRNA) is enrolled in CC process. This study aims to unveil the profiles of circ_101119 (circCDK17) in cell proliferation, migration, invasion, apoptosis and glycolysis in CC.MethodsThe expression levels of circCDK17, microRNA-1294 (miR-1294) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) mRNA were detected by quantitative real time polymerase chain reaction (qRT-PCR). The protein expression levels of YWHAZ, recombinant glucose transporter 1 (GLUT1) and hexokinase 2 (HK2) were determined by western blot. Cell proliferation, migratory and invasive abilities and apoptosis were illustrated by cell counting kit-8 (CCK-8) assay, transwell assay and flow cytometry analysis, respectively. Cell lactate production, glucose uptake and adenosine 5'-triphosphate (ATP) level were severally elucidated by lactate assay kit, glucose assay kit and ATP detection kit.ResultsCircCDK17 expression and the mRNA and protein expression levels of YWHAZ were dramatically upregulated, while miR-1294 expression was obviously downregulated in CC tissues or cells compared with control groups. CircCDK17 silencing suppressed cell proliferation, migration, invasion and glycolysis, and induced cell apoptosis in CC; however, miR-1294 inhibitor restrained these effects. Additionally, circCDK17 was a sponge of miR-1294 and miR-1294 bound to YWHAZ. Furthermore, circCDK17 knockdown inhibited tumor formation in vivo.ConclusionCircCDK17 knockdown repressed cell proliferation, migration, invasion and glycolysis, and promoted cell apoptosis via miR-1294/YWHAZ axis in CC. This finding provides a theoretical basis in studying circRNA-mediated therapy in CC.
Assessing the effectiveness of a community health advisor plus screen to save educational intervention on stool-based testing adherence in an African American safety net clinic population: study protocol for a randomized pragmatic trial
Authors: Luque John S. , Matthew Olayemi O. , Jackson Deloria R. , Vargas Matthew A. , Austin Tifini , Ali Askal , Kiros Gebre E. , Harris Cynthia M. , Tawk Rima , Gwede Clement K. , Wallace Kristin , Jean-Pierre Pascal 
Published: February 2022

AbstractBackgroundColorectal cancer (CRC) is the second most deadly cancer affecting US adults and is also one of the most treatable cancers when detected at an earlier clinical stage of disease through screening. CRC health disparities experienced by African Americans are due in part to the later stage of diagnosis, suggesting the importance of improving African Americans’ CRC screening participation. The national Screen to Save (S2S) initiative employs a community health educator to deliver CRC screening education which can be tailored for specific populations, and such approaches have increased CRC screening rates in disadvantaged and racial/ethnic minority populations.Methods/designIn this trial emphasizing stool-based CRC screening, focus groups informed the development of an adapted S2S video and brochure tailored for African Americans and identified preferred motivational text messages for a multicomponent community health advisor (CHA) intervention. A CHA hired from the community was trained to deliver a 6-week CRC educational intervention consisting of an initial face-to-face meeting followed by 5 weeks of calls and texts. Interested eligible persons are enrolled primarily through recruitment by two partnering community health centers (CHCs) and secondarily through various outreach channels and, after consenting and completing a baseline survey, are randomly assigned to one of two study arms. The CHCs are blinded to study arm assignment. Intervention arm participants receive the brochure and CHA intervention while participants assigned to the control group receive only the brochure. All participants receive a stool-based CRC screening test from their health center, and the primary outcome is the completion of the screening test at 12 months. Secondary objectives are to estimate the effect of the intervention on mediating factors, explore the effect of moderating factors, and perform a cost-effectiveness analysis of the CHA intervention.DiscussionThe TUNE-UP study will enhance understanding about CRC screening in African Americans obtaining primary health care through CHCs and is one of the very few studies to examine a CHA intervention in this context. A better understanding of the mechanisms by which the intervention affects patient beliefs and behaviors will help focus future research while the exploratory cost-effectiveness analysis will inform CHCs’ decision-making about implementing a CHA program to increase screening and reduce cancer health disparities.Trial registrationClinicalTrials.govNCT04304001. Registered on March 11, 2020.
The effects of acupuncture on psychological symptoms in patients with insomnia: study protocol for a randomized controlled trial
Authors: Wang Lifen , Wang Ruisen , Yao Yanling , Bai Xue , Sheng Gang 
Published: February 2022

Abstract
Background
Insomnia is a common sleep-related condition that includes dissatisfaction with sleep quality, difficulty in initiating or maintaining sleep, and early morning waking. Insomnia can affect daytime functioning by causing fatigue, depression, and anxiety. Medications are the most common method for the management of insomnia but can cause adverse effects, including psychological and physical dependence, residual daytime sedation, and cognitive impairment. Acupuncture is a common traditional Chinese therapy. It has been used in the treatment of insomnia, depression, and anxiety in China. However, there are no high-quality studies focusing on acupuncture for insomnia, especially for depression and anxiety due to insomnia. Therefore, we have designed a randomized controlled trial (RCT) involving a placebo control to ensure blinding of participants to investigate the effects of acupuncture on insomnia in improving sleep quality and psychosocial symptoms.

Methods
We have designed a single-center, parallel-group, single-blinded RCT. A total of 252 participants who meet the eligibility criteria will be randomly allocated into a manual acupuncture group or sham acupuncture group in a 1:1 ratio. All participants will receive 24 sessions of acupuncture (30 min per session, three sessions per week for 8 weeks). Participants will be assessed using the Pittsburgh Sleep Quality Index score, self-assessment anxiety scale, self-assessment depression scale, and Medical Outcomes Study 36-Item Short-Form Health Survey at baseline and 8 weeks. All analyses will be based on an intention-to-treat principle. The results will be published in an international peer-reviewed journal.

Discussion
The results of this study are expected to clarify the effects of acupuncture on sleep quality and psychosocial symptoms in patients with insomnia. This will contribute to the clinical practice of acupuncture in the management of insomnia.

Trial registration
Chinese Clinical Trail Registry ChiCTR2100049172. Registered on 24 July 2021.
Optimization of an economical medium composition for the coculture of Clostridium butyricum and Bacillus coagulans
Authors: Li Yonghong , Wang Yun , Liu Yingying , Li Xuan , Feng Lifei , Li Keke 
Published: February 2022

AbstractClostridium butyricum is a spore-forming probiotic which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is 10 g/L bran, 15 g/L corn steep powder, 15 g/L peptone, 1 g/L K2HPO4 and 0.5 g/L MnSO4.Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached 1.5 × 108 cfu/mL, Which was 375 times higher than that incubated in the initial medium. The sporulation rate reach 92.6%. The results revealed an economical and effective medium composition for the coculture of C. butyricum and B. coagulans, which achieved a 64.6% cost reduction. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.
Physicians’ perceptions and preferences for implementing venous thromboembolism (VTE) clinical practice guidelines: a qualitative study using the Theoretical Domains Framework (TDF)
Authors: Abboud Juliana , Rahman Abir Abdel , Shaikh Niaz , Dempster Martin , Adair Pauline 
Published: February 2022

AbstractBackgroundVenous thromboembolism is a primary cause of morbidity and mortality in hospitalised patients. Clinical practice guidelines were developed to prevent venous thromboembolism events. This study adopted the Theoretical Domains Framework to explore the beliefs and perceptions of physicians adoption of clinical practice guidelines for the uptake of venous thromboembolism prevention guidelines.MethodsSemi-structured interviews were conducted with a stratified purposive sample of internal medicine physicians in an acute hospital. The interview topic guide was developed using the Theoretical Domains Framework to identify the factors perceived to influence the practice. Two researchers coded the interview transcripts using thematic content analysis. Emerging relevant themes were mapped to TDF domains.ResultsA total of sixteen medical physicians were interviewed over a six-month period. Nine theoretical domains derived from thirty-three belief statements were identified as relevant to the target behaviour; knowledge (education about the importance of VTE guidelines); beliefs about capabilities (with practice VTE tool easier to implement); beliefs about consequences (positive consequences in reducing the development of VTE, length of stay, financial burden and support physician decision) and (negative consequence risk of bleeding); reinforcement (recognition and continuous reminders); goals (patient safety goal); environmental context and resources (workload and availability of medications were barriers, VTE coordinator and electronic medical record were enablers); social influences (senior physicians and patient/family influence the VTE practice); behavioural regulation (monitoring and mandatory hospital policy); and nature of the behaviour.ConclusionsUsing the Theoretical Domains Framework, factors thought to influence the implementation of VTE clinical practice guidelines were identified which can be used to design theoretically based interventions by targeting specific psychological constructs and linking them to behaviour change techniques to change the clinical practice of physicians.
The cultivation of five microalgae species and their potential for biodiesel production
Authors: Rodríguez-Palacio Mónica Cristina , Cabrera-Cruz René Bernardo Elías , Rolón-Aguilar Julio Cesar , Tobías-Jaramillo Ricardo , Martínez-Hernández Marisol , Lozano-Ramírez Cruz 
Published: February 2022

Abstract
Background
Due to the problems we face today, such as wastewater pollution of aquifers and climate change, it is necessary to search for environmental solutions that help us minimize this problem. An alternative solution might be the cultivation of microalgae that are efficient in the purification of wastewater, removal of greenhouse gases and production of biomass that can be used for the production of biofuels such as biodiesel, methane, bioethanol, among others. The aim of this work is to cultivate five strains of microalgae native in Mexico: Chlorella miniata, Coelastrella sp., Desmodesmus quadricauda, Neochloris oleoabundans and Verrucodesmus verrucosus. The cultivations were performed using municipal wastewater and a foliar fertilizer with the further purpose of assessing their capacity to produce various types of biomass, in particular lipids.

Methods
The experiments were carried out using triplicate 16-L glass bioreactors assays with a 12:12 light–darkness cycle at 25 °C ± 1 under constant aeration. Every 3rd day, a 1-mL sample was taken to determine cell density. In the stationary growth phase, each culture was harvested by sedimentation and lipid content analysis was performed. The biomass with the highest concentration of total lipids was subjected to an analysis of the methyl esters of fatty acids.

Results
An ANOVA test showed significant differences between the growth rates (F = 6.8, p = 0.0001). The species that were able to produce biomass with the highest concentrations of total lipids were Coelastrella sp. with 44–46%; Verrucodesmus verrucosus with 43–44% and Neochloris oleoabundans 35–37%. As the analysis of the methyl esters of fatty acids showed, the species Coelastrella sp. and V. verrucosus produced lipids composed of 82.9% and 91.28% of fatty acids, respectively, containing C16–C18 carbon chains.

Conclusions
All the species used in the present study were able to grow on wastewater and produce high concentrations of lipids. Therefore, the demands for biodiesel production could be met in the immediate future after continuing working with different microalgae species. Therefore, it is necessary to determine their adaptation potential to grow on contaminated effluents and produce lipids that can be used for the benefit of people and environment.
Postoperative decrease of albumin (ΔAlb) as early predictor of complications after gastrointestinal surgery: a systematic review
Authors: Joliat Gaëtan-Romain , Schoor Arnaud , Schäfer Markus , Demartines Nicolas , Hübner Martin , Labgaa Ismail 
Published: February 2022

Abstract
Background
Postoperative complications are frequent after gastrointestinal surgery and early prediction remains an unmet need. Serum albumin shows a rapid decrease after surgery, and this decline (ΔAlb) may reflect the intensity of the surgical stress response and thereby be a predictor of postoperative complications. This study aimed to comprehensively review the available data on ΔAlb in gastrointestinal surgery.

Methods
PRISMA guidelines were followed to conduct a systematic review of the literature in MEDLINE and Embase. Studies assessing the role of ΔAlb to predict complications after gastrointestinal surgery were included.

Results
A total of 1256 articles were screened, and 16 studies were included in the final analysis: 7 prospective and 9 retrospective trials. Sensitivity of ΔAlb to predict postoperative complications ranged from 63 to 84%, whereas specificity ranged from 61 to 86%. Nine out of the 16 included studies established a threshold of ΔAlb to predict morbidity (range: 5–11 g/l or 14–27%).

Conclusion
ΔAlb appeared as a valuable and promising biomarker to anticipate complications after gastrointestinal surgery. Future efforts are needed to determine whether and how ΔAlb may be integrated in clinical practice to guide clinicians in the perioperative management of patients.
A review of Japanese-style bathing: its demerits and merits
Authors: Tochihara Yutaka 
Published: February 2022

AbstractJapanese-style bathing (JSB), which involves soaking in hot water up to the shoulders in deep bathtubs for a long time in the evening to night, is unique. Many experimental and epidemiological studies and surveys have shown that JSB improve sleep quality, especially shortens sleep onset latency in winter. In addition, repeated JSB lead the improvement of depressive symptoms. JSB is a simple and low-cost non-pharmacological measure to sleep difficulty in winter and mental disorders, especially for the elderly. On the contrary, drowning, while soaking in a bathtub, is the most common of accidental death at home in Japan. It is estimated that approximately 19,000 Japanese individuals die annually while taking a bath, mostly during winter, and most victims are elderly people. Elderly Japanese people tend to prefer a higher-risk JSB because the temperature inside the house during winter, especially the dressing room/bathroom temperature, is very low. Since the physiological thermal effect of the elderly associated with bathing is relatively lower among the elderly than the young, the elderly prefer to take a long hot bath. This elderly’s favorite style of JSB results in larger increased blood pressure in dressing rooms and larger decreased in blood pressure during hot bathing. A sudden drop in blood pressure while immersed in the bathtub leads to fainting and drowning. Furthermore, elderly people are less sensitive to cold air or hot water, therefore, it is difficult to take appropriate measures to prevent large fluctuations in blood pressure. To ensure a safe and comfortable winter bathing, the dressing room/bathroom temperature needs to be maintained at 20 °C or higher, and several degrees higher would be recommended for the elderly.
Terminal anorexia nervosa: three cases and proposed clinical characteristics
Authors: Gaudiani Jennifer L. , Bogetz Alyssa , Yager Joel 
Published: February 2022

AbstractBackgroundMost individuals with eating disorders will either recover, settle into an unrecovered but self-defined acceptable quality of life, or continue to cycle from crisis to relative stability over time. However, a minority of those with severe and enduring eating disorders recognize after years of trying that recovery remains elusive, and further treatment seems both futile and harmful. No level of harm reduction proves achievable or adequately ameliorates their suffering. In this subgroup, many of those with anorexia nervosa will experience the medical consequences of malnutrition as their future cause of death. Whereas anyone who wishes to keep striving for recovery despite exhaustion and depletion should wholeheartedly be supported in doing so, some patients simply cannot continue to fight. They recognize that death from anorexia nervosa, while perhaps not welcome, will be inevitable. Unfortunately, these patients and their carers often receive minimal support from eating disorders health professionals who are conflicted about terminal care, and who are hampered and limited by the paucity of literature on end-of-life care for those with anorexia nervosa.Case presentationThree case studies elucidate this condition. One patient was so passionate about this topic that she asked to be a posthumous co-author of this paper.ConclusionsConsistent with literature on managing terminal illness, this article proposes clinical characteristics of patients who may be considered to have a terminal eating disorder: diagnosis of anorexia nervosa, older age (e.g. age over 30), previous participation in high quality care, and clear and consistent determination by a patient who possesses decision-making capacity that additional treatment would be futile, knowing their actions will result in death. By proposing the clinical characteristics of terminal anorexia nervosa, we hope to educate, inspire compassion, and help providers properly assess these patients and provide appropriate care. We hope that this proposal stimulates further expert consensus definitions and clinical guidelines for management of this population. In our view, these patients deserve the same attendant care and rights as all other patients with terminal illness, up to and including medical aid in dying in jurisdictions where such care is legal.
Role of strain echocardiography in patients with hypertension
Authors: Oh Jin Kyung , Park Jae-Hyeong 
Published: February 2022

AbstractHypertension is a well-recognized risk factor for the development of cardiovascular disease, and the early detection of cardiac changes from hypertension can allow reversing these. Hypertensive heart diseases (HHD) refer to the complex and diverse change of the cardiac structure and function secondary to hypertension. Although conventional echocardiography is the most common imaging modality in detecting HHD, it cannot detect subtle changes of cardiac structure in subclinical states. Because strain echocardiography is another echocardiographic modality can detect subclinical myocardial dysfunction by measuring intrinsic myocardial deformation, it became more and more popular in clinical and research fields. In this review article, we described the basic concept of strain echocardiography and summarized several clinical studies showing its clinical utilities in the detection of HHD.
Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases
Authors: Kawada Taishu , Kino Katsuhito , Tokorodani Kyousuke , Anabuki Ryuto , Morikawa Masayuki , Kobayashi Takanobu , Ohara Kazuaki , Ohshima Takayuki , Miyazawa Hiroshi 
Published: February 2022

Abstract
Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua. We report here the following nucleotide incorporations opposite Ua by various DNA polymerases: DNA polymerase α, dATP and dGTP (dATP > dGTP); DNA polymerase δ, dATP; DNA polymerase ζ, dATP; Kf exo−, dATP; Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), dGTP and dATP (dGTP > dATP); and DNA polymerase η, dCTP, dGTP, dATP, and dTTP (dCTP > dGTP > dATP > dTTP). DNA polymerases β and ε were blocked by Ua. Elongation by DNA polymerases δ and ζ stopped after inserting dATP opposite Ua. Importantly, the elongation efficiency to full-length beyond Ua using DNA polymerase η and Dpo4 were almost the same as that of natural DNA.

Graphical abstract
Why many African countries may not achieve the 2022 COVID-19 vaccination coverage target
Authors: Idris Israel Oluwaseyidayo , Ayeni Gabriel Omoniyi , Adebisi Yusuff Adebayo 
Published: February 2022

AbstractCOVID-19 continues to strain, stress, and stretch health systems globally. With the development of the COVID-19 vaccines, there are many issues still lurking behind the widespread coverage; one of which is COVID-19 vaccine nationalism and African countries are not exempted from these issues. This is evident in that many countries in the African region missed the earlier targets set by World Health Organization (WHO) for COVID-19 vaccination coverage. The WHO further set a target of 70% coverage of the COVID-19 vaccines for all countries by June 2022. In this article, we discuss the possible reasons why many African countries are struggling and may not achieve the COVID-19 vaccination target in 2022. With the fundamental issues facing COVID-19 vaccination ranging from nationalism to hesitancy, it is important that stakeholders continue to work harder to ensure that the continent is not left behind in the race to keep the world free and safe from the sting of the COVID-19 pandemic.