bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integration of multi‐omics data reveals interplay between brassinosteroid and Target of Rapamycin Complex signaling in Arabidopsis
Authors: Montes Christian , Wang Ping , Liao Ching‐Yi , Nolan Trevor M. , Song Gaoyuan , Clark Natalie M. , Elmore J. Mitch , Guo Hongqing , Bassham Diane C. , Yin Yanhai , Walley Justin W. 
Published: July 2022

Summary

Brassinosteroids (BRs) and Target of Rapamycin Complex (TORC) are two major actors coordinating plant growth and stress responses. Brassinosteroids function through a signaling pathway to extensively regulate gene expression and TORC is known to regulate translation and autophagy. Recent studies have revealed connections between these two pathways, but a system‐wide view of their interplay is still missing.

We quantified the level of 23 975 transcripts, 11 183 proteins, and 27 887 phosphorylation sites in wild‐type Arabidopsis thaliana and in mutants with altered levels of either BRASSINOSTEROID INSENSITIVE 2 (BIN2) or REGULATORY ASSOCIATED PROTEIN OF TOR 1B (RAPTOR1B), two key players in BR and TORC signaling, respectively.

We found that perturbation of BIN2 or RAPTOR1B levels affects a common set of gene‐products involved in growth and stress responses. Furthermore, we used the multi‐omic data to reconstruct an integrated signaling network. We screened 41 candidate genes identified from the reconstructed network and found that loss of function mutants of many of these proteins led to an altered BR response and/or modulated autophagy activity.

Altogether, these results establish a predictive network that defines different layers of molecular interactions between BR‐ or TORC‐regulated growth and autophagy.
Unraveling the intestinal epithelial barrier in cyanotoxin microcystin‐treated Caco‐2 cell monolayers
Authors: Kaak Jan‐Leo , Lobo de Sá Fábia D. , Turner Jerrold R. , Schulzke Jörg‐Dieter , Bücker Roland 
Published: July 2022

AbstractMicrocystin is a widespread cyanobacterial toxin that affects the intestine to produce diarrheal symptoms after ingestion of freshwater blue‐green algae. Our study aimed to characterize the mechanism by which the toxin leads to diarrhea via epithelial barrier dysfunction in a small intestine Caco‐2 cell model. Microcystin‐treated human Caco‐2 epithelial monolayers were functionally and molecularly analyzed for barrier dysfunction. Tight junctions (TJs) and cell damage were analyzed in relation to transepithelial electrical resistance (TER) changes. TER of microcystin‐treated Caco‐2 cells was reduced by 65% of the initial value after 24 h; concomitantly, permeability for fluorescein increased 2.6‐fold. Western blot analysis showed reduced claudin‐1 expression, while expression of claudin‐3 and ‐4 remained unchanged. Super‐resolution stimulated emission depletion microscopy revealed that TJ integrity was compromised by fraying and splitting of the TJ domain of the epithelial cells. Epithelial apoptosis did not significantly contribute to epithelial barrier dysfunction, while cytoskeletal actomyosin constriction was associated with TJ disintegration and the barrier defect. Our results indicate that microcystin causes intestinal barrier leakiness, which helps to explain the leak flux type of diarrhea as the main pathomechanism after ingestion of cyanobacterial toxin.
Customized Multilevel 3D Printing Implant for Reconstructing Spine Tumor: A Retrospective Case Series Study in a Single Center
Authors: Sun Zhengwang , Yin Mengchen , Sun Yueli , Cheng Mo , Fang Meng , Huang Wending , Ma Junming , Yan Wangjun 
Published: July 2022

AbstractObjectiveTo investigate the clinical efficacy and safety of 3D printed artificial vertebral body for patients who underwent multilevel total en bloc spondylectomy (TES) and analyze whether it could reduce the incidence of implant subsidence.MethodsThis is a retrospective study. From January 2017 to May 2018, eight consecutive cases with spine tumor undergoing multilevel TES were analyzed. All patients underwent X‐ray and CT examinations to evaluate the stability of internal fixation during the postoperative follow‐up. Demographic, surgical details, clinical data, and perioperative complications was collected. Visual analog scale, Frankel score, and spinal instability neoplastic score (SINS) classification were also recorded.ResultsThere were six cases of primary spinal tumor and two cases of metastatic spinal tumor. All patients achieved remarkable pain relief and improvement in neurological function. Five patients underwent operation through the posterior approach, one patient underwent operation through the anterior approach and the remaining two patients through a combined anterior and posterior approach. At the last follow‐up period, X‐rays showed that the 3D printed artificial vertebral body of all cases matched well, and the fixation was reliable. Hardware failure such as loosening, sinking, breaking, and displacement wasn't observed during the follow‐up period.Conclusion3D printed customized artificial vertebral body can provide satisfying good clinical and radiological outcomes for patients who have undergone multilevel TES.
A consensus statement on health‐care transition for childhood‐onset inflammatory bowel disease patients
Authors: Kumagai Hideki , Shimizu Toshiaki , Iwama Itaru , Hagiwara Shin‐Ichiro , Kudo Takahiro , Takahashi Michiko , Saito Takeshi , Kunisaki Reiko , Uchino Motoi , Hiraoka Sakiko , Naganuma Makoto , Sugimoto Ken , Miyoshi Jun , Shibuya Tomoyoshi , Hisamatsu Tadakazu 
Published: July 2022

AbstractInflammatory bowel disease (IBD) is a chronic relapsing inflammatory disorder of the intestine. The incidence of IBD is increasing worldwide, including Japan, and in approximately 25% of all affected patients it is diagnosed before 18 years of age. For the health maintenance of such patients, planned transition to adult care systems is essential. Previous Japanese surveys have revealed gaps between adult and pediatric gastroenterologists with regard to their knowledge and perception of health‐care transition for patients with childhood‐onset IBD. In 2021–2022, several Web workshops to discuss issues related to the transitional care of IBD patients were held by the Ministry of Health, Labour and Welfare of Japan as part of their program for research on intractable diseases. Clinicians experienced in IBD treatment for pediatric and adult patients participated. As a result, this panel of adult and pediatric gastroenterologists developed five consensus statements on the issue of “transfer from pediatric to adult care” and nine statements on the issue of “addressing transitional care (transition program).” To address current gaps in health‐care transition for childhood‐onset IBD patients, a programmed approach to transition, and better partnerships between pediatric and adult gastroenterologists are indicated. It is hoped that this consensus statement will provide a basis for the development of appropriate guidelines for clinical practice.
Foreknowledge requires determinism
Authors: Todd Patrick 
Published: July 2022

AbstractThere is a longstanding argument that purports to show that divine foreknowledge is inconsistent with human freedom to do otherwise. Proponents of this argument, however, have for some time been met with the following reply: the argument posits what would have to be a mysterious non‐causal constraint on freedom. In this paper, I argue that this objection is misguided—not because after all there can indeed be non‐causal constraints on freedom (as in Pike, Fischer, and Hunt), but because the success of the incompatibilist's argument does not require the real possibility of non‐causal constraints on freedom. I contend that the incompatibilist's argument is best seen as showing that, given divine foreknowledge, something makes one unfree—and that this something is most plausibly identified, not with the foreknowledge itself, but with the causally deterministic factors that would have to be in place in order for there to be infallible foreknowledge in the first place.
Assumption-lean Inference for Generalised Linear Model Parameters
Authors: Vansteelandt Stijn , Dukes Oliver 
Published: July 2022

AbstractInference for the parameters indexing generalised linear models is routinely based on the assumption that the model is correct and a priori specified. This is unsatisfactory because the chosen model is usually the result of a data-adaptive model selection process, which may induce excess uncertainty that is not usually acknowledged. Moreover, the assumptions encoded in the chosen model rarely represent some a priori known, ground truth, making standard inferences prone to bias, but also failing to give a pure reflection of the information that is contained in the data. Inspired by developments on assumption-free inference for so-called projection parameters, we here propose novel nonparametric definitions of main effect estimands and effect modification estimands. These reduce to standard main effect and effect modification parameters in generalised linear models when these models are correctly specified, but have the advantage that they continue to capture respectively the (conditional) association between two variables, or the degree to which two variables interact in their association with outcome, even when these models are misspecified. We achieve an assumption-lean inference for these estimands on the basis of their efficient influence function under the nonparametric model while invoking flexible data-adaptive (e.g. machine learning) procedures.
Pentoxifylline and tocopherol for prevention of osteoradionecrosis in patients who underwent oral surgery: A clinical audit
Authors: Lombardi Niccolò , Varoni Elena , Villa Giulio , Salis Annalisa , Lodi Giovanni 
Published: July 2022

AbstractPurposeOsteoradionecrosis (ORN) of the jaw is a severe and debilitating complication of the head and neck radiotherapy which frequently occurrs after oral surgery. This clinical audit aims aevaluate the effectiveness of combined use of pentoxifylline and tocopherol (PENTO) in prevention ORN onset in patient who underwent oral surgery after head and neck radiotherapy (RT).Material methodIn this clinical audit Pentoxifylline 400 mg, twice a day, and Tocopherol 800 IU once a day (PENTO protocol) have been prescribed. Patients started the protocol 1 week before the surgical procedure and continued for 8 weeks after.ResultsTwenty‐nine patients were included. They received 75 surgical interventions under PENTO protocol: 71 surgical procedures of dental extraction (single or multiple dental extractions in each session) and four implant placements. A total of 152 dental extractions were carried out: 64 surgical extractions which required the raising of mucoperiosteal flap, and 88 simple extractions. Four out of 29 patients developed ORN after surgical procedures: four cases of ORN occurred after dental extractions (5.6%) and one case of ORN after implant placement (25%).ConclusionPENTO is a useful ORN preventive protocol, low‐cost and clinically feasible, safe and well tolerated by patients. Further studies should focus on better defining the effectiveness PENTO, independently from the antibiotic therapy.
Poverty, social work, and radical incrementalism: Current developments of the poverty‐aware paradigm
Authors: Krumer‐Nevo Michal 
Published: July 2022

AbstractFacing the growing domination of neoliberalism and new public management in welfare services, social work scholars and practitioners are engaged in efforts to resist it. Using Schram's concept of radical incrementalism, this article describes and analyzes the development of the Poverty‐Aware Paradigm (PAP) in Israel in light of this resistance. The PAP is a critical framework that focuses on poverty as a central axis of the analysis of power relations between service users and social workers. Since 2015, when the Israeli welfare ministry adopted the PAP as a leading model for social work practice in the social service departments, the paradigm has had a surprisingly warm reception, and its implementation has been extended to the health and justice systems as well as to additional welfare ministry units. The article discusses these developments as examples of ‘radical incrementalism’ that is, strategies of resisting neoliberalism by collaborating with the establishment to ‘bend’ the power of the state to benefit marginalized groups. The article analyzes the contribution of specific elements of the PAP‐its dialectic process of bottom‐up and top‐down development; its discursive contribution; its organizational framework; and its training model. Finally, the article discusses the radical nature of the PAP.
Grass‐specific ABERRANT MICROSPORE DEVELOPMENT 1 is required for maintaining pollen fertility in rice
Authors: Zou Ting , Xiong Pingping , Zhou Fuxing , Zhou Dan , Chen Hao , Li Gongwen , Peng Kun , Zheng Kaiyou , Han Yuhao , Zhang Kaixuan , Zhang Xu , Yang Shangyu , Deng Qiming , Wang Shiquan , Zhu Jun , Liang Yueyang , Sun Changhui , Yu Xiumei , Liu Huainian , Wang Lingxia , Li Ping , Li Shuangcheng 
Published: July 2022

SUMMARYPollen development includes a series of biological events that require precise gene regulation. Although several transcription factors (TFs) have been shown to play roles in maintaining pollen fertility, the major regulatory networks underlying tapetum development and pollen wall formation are largely unknown. Herein, we report that ABERRANT MICROSPORE DEVELOPMENT1 (AMD1), a protein annotated previously as unknown protein, is required for tapetum development and pollen exine patterning in rice (Oryza sativa L.). AMD1 encodes a grass‐specific protein exhibiting transactivation activity in the nucleus and is spatiotemporally expressed in the tapetum and microspores during pollen development. Further biochemical assays indicate that AMD1 directly activates the transcription of DEFECTIVE POLLEN WALL (DPW) and POLYKETIDE SYNTHASE2 (OsPKS2), which are both implicated in sporopollenin biosynthesis during exine formation. Additionally, AMD1 directly interacts with TAPETUM DEGENERATION RETARDATION (TDR), a key TF involved in the regulation of tapetum degradation and exine formation. Taken together, we demonstrate that AMD1 is an important regulatory component involved in the TDR‐mediated regulatory pathway to regulate sporopollenin biosynthesis, tapetum degradation, and exine formation for pollen development. Our work provides insights into the regulatory network of rice sexual reproduction and a useful target for genetic engineering of new male‐sterile lines for hybrid rice breeding.
The potential therapeutic benefits of low frequency haemodynamic oscillations
Authors: Anderson Garen K. , Rickards Caroline A. 
Published: July 2022

AbstractHaemodynamic oscillations occurring at frequencies below the rate of respiration have been observed experimentally for more than a century. Much of the research regarding these oscillations, observed in arterial pressure and blood flow, has focused on mechanisms of generation and methods of quantification. However, examination of the physiological role of these oscillations has been limited. Multiple studies have demonstrated that oscillations in arterial pressure and blood flow are associated with the protection in tissue oxygenation or functional capillary density during conditions of reduced tissue perfusion. There is also evidence that oscillatory blood flow can improve clearance of interstitial fluid, with a growing number of studies demonstrating a role for oscillatory blood flow to aid in clearance of debris from the brain. The therapeutic potential of these haemodynamic oscillations is an important new area of research which may have beneficial impact in treating conditions such as stroke, cardiac arrest, blood loss injuries, sepsis, or even Alzheimer's disease and vascular dementia.
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Future Leadership
Authors: Bryant-Lukosius Denise 
Published: July 2022

To coin a phrase from the lyrics of an old song, “what the world needs now” to improve global health is nursing leadership. Several policy reports from the World Health Organization1,2 and the Pan America Health Organization (PAHO)3 emphasize the strategic importance of nursing leadership and the strengthening of nursing leadership at all levels of health systems for improving access and quality of care and population health outcomes. Similarly, these reports recognize the enhanced clinical expertise provided by advanced practice nurses and advocate for their expanded use and optimized scope of practice to deliver needed health care services in all countries, but especially in lower- and middle-income countries where advanced practice nurses may be few or non-existent, and where there is high health inequity.
Guanidinoacetic acid provides superior cardioprotection to its combined use with betaine and (or) creatine in HIIT-trained rats
Authors: Prokic Veljko Z. , Rankovic Marina R. , Draginic Nevena D. , Andjic Marijana M. , Sretenovic Jasmina Z. , Zivkovic Vladimir I. , Jeremic Jovana N. , Milinkovic Milica V. , Bolevich Sergey , Jakovljevic Vladimir L.J. , Pantovic Suzana B. 
Published: July 2022

This study aimed to determine how guanidinoacetic acid (GAA) or its combined administration with betaine (B) or creatine (C) influences the cardiac function, morphometric parameters, and redox status of rats subjected to high-intensity interval training (HIIT). This research was conducted on male Wistar albino rats exposed to HIIT for 4 weeks. The animals were randomly divided into five groups: HIIT, HIIT + GAA, HIIT + GAA + C, HIIT + GAA + B, and HIIT + GAA + C + B. After completing the training protocol, GAA (300 mg/kg), C (280 mg/kg), and B (300 mg/kg) were applied daily per os for 4 weeks. GAA supplementation in combination with HIIT significantly decreased the level of both systemic and cardiac prooxidants ([Formula: see text], H2O2, [Formula: see text], and thiobarbituric acid reactive substances) compared with nontreated HIIT ( p < 0.05). Also, GAA treatment led to an increase in glutathione and superoxide dismutase levels. None of the treatment regimens altered cardiac function. A larger degree of cardiomyocyte hypertrophy was observed in the HIIT + GAA group, which was reflected through an increase of the cross-sectional area of 27% ( p < 0.05) and that of the left ventricle wall thickness of 27% ( p < 0.05). Since we showed that GAA in combination with HIIT may ameliorate oxidative stress and does not alter cardiac function, the present study is a basis for future research exploring the mechanisms of cardioprotection induced by this supplement in an HIIT scenario.
ALP-portal majorana dark matter
Authors: Gola Shivam , Mandal Sanjoy , Sinha Nita 
Published: July 2022

Axion like particles (ALPs) and right-handed neutrinos (RHNs) are two well-motivated dark matter (DM) candidates. However, these two particles have a completely different origin. Axion was proposed to solve the strong CP problem, whereas RHNs were introduced to explain light neutrino masses through seesaw mechanisms. We study the case of ALP portal RHN DM (Majorana DM) taking into account existing constraints on ALPs. We consider the leading effective operators mediating interactions between the ALP and Standard Model (SM) particles and three RHNs to generate light neutrino masses through type-I seesaw. Further, ALP-RHN neutrino coupling is introduced to generalize the model which is restricted by the relic density and indirect detection constraint.
Impact of generalized fourier law in thermal flux convective flow over a vertical plate: Analysis of fractional derivative
Authors: Raza Ali , Hejazi Hala A. , Khan M. Ijaz 
Published: July 2022

In this paper, a convective fluid flow mixed with different nanoparticles (NPs) flowing on a vertical plate is studied. The impacts of slip boundary wall, Newtonian heating, and magnetic field are also pondered. Using Fourier’s rule of heat conductivity and Caputo-time (CT) fractional derivative (FD), the nondimensional governing equations for this mathematical model are obtained. The Laplace transformation (LT) technique is used to get a generalized solution to the equations of momentum and energy. The physical interpretation of velocity distribution in some particular cases is well recognized in the past research works and is also highlighted to scrutinize some further physical perception into the subject and to fortify the novelty of this research communication. Furthermore, graphical depictions are created using the mathematica program to assess the physical importance of the considered topic. As a result, we concluded that the blood-based aluminium oxide ([Formula: see text] NPs show more decaying behavior as compared to titanium dioxide ([Formula: see text] NPs for temperature and velocity profile. Moreover, changing the fractional parameter’s value for a long period of time improves thermal and velocity profiles.
High-Performance Polynomial Root Finding for Graphics
Authors: Yuksel Cem 
Published: July 2022

We present a computationally-efficient and numerically-robust algorithm for finding real roots of polynomials. It begins with determining the intervals where the given polynomial is monotonic. Then, it performs a robust variant of Newton iterations to find the real root within each interval, providing fast and guaranteed convergence and satisfying the given error bound, as permitted by the numerical precision used.
For cubic polynomials, the algorithm is more accurate and faster than both the analytical solution and directly applying Newton iterations. It trivially extends to polynomials with arbitrary degrees, but it is limited to finding the real roots only and has quadratic worst-case complexity in terms of the polynomial's degree.
We show that our method outperforms alternative polynomial solutions we tested up to degree 20. We also present an example rendering application with a known efficient numerical solution and show that our method provides faster, more accurate, and more robust solutions by solving polynomials of degree 10.
Temporally Stable Real-Time Joint Neural Denoising and Supersampling
Authors: Thomas Manu Mathew , Liktor Gabor , Peters Christoph , Kim Sungye , Vaidyanathan Karthik , Forbes Angus G. 
Published: July 2022

Recent advances in ray tracing hardware bring real-time path tracing into reach, and ray traced soft shadows, glossy reflections, and diffuse global illumination are now common features in games. Nonetheless, ray budgets are still limited. This results in undersampling, which manifests as aliasing and noise. Prior work addresses these issues separately. While temporal supersampling methods based on neural networks have gained a wide use in modern games due to their better robustness, neural denoising remains challenging because of its higher computational cost.
We introduce a novel neural network architecture for real-time rendering that combines supersampling and denoising, thus lowering the cost compared to two separate networks. This is achieved by sharing a single low-precision feature extractor with multiple higher-precision filter stages. To reduce cost further, our network takes low-resolution inputs and reconstructs a high-resolution denoised supersampled output. Our technique produces temporally stable high-fidelity results that significantly outperform state-of-the-art real-time statistical or analytical denoisers combined with TAA or neural upsampling to the target resolution.