bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of the TLR4/NF-κB inflammatory pathway decreases the microglia-mediated overpruning of synapses to ameliorate the mechanism underlying brain injury after epileptic seizures
Authors: Wu Qiong , Wang Hua , Liu Xueyan , Zhao Yajuan , Zhao Xin , Su Peng , Miao Jianing 
Published: September 2022

Abstract
Objective: Brain injury after the onset of epilepsy is a neurological symptom that urgently needs to be addressed, and there is currently no effective treatment. Excessive synaptic pruning is one of the most important causes of this symptom, but the specific underlying mechanism is not clear. Methods: A pilocapine-induced rat model of epilepsy was established, and the rats were randomly divided into 4 groups: a control group, Epilepsy group, TLR4 inhibitor group (Epilepsy+TAK-242 group), and NF-κB antagonist group (Epilepsy+BAY11-7082 group). Semeiology was used to observe epilepsy in the rats, and conducted experiments Results: 1. Frequency of epileptic seizures and epileptic seizure grade: Compared with those in the EP group, the frequency and intensity of epileptic seizures in the EP+BAY group and the EP+TAK group were reduced to different degrees. 2. The Nissl staining results showed that the number of neurons in the TLR4 inhibitor group and NF-κB antagonist group was significantly higher than that in the epilepsy group. 3. CX3CR1 was most highly expressed in the Epilepsy group, with a statistically significant difference compared with CX3CR1 expression in the Ctrl, EP+BAY and EP+TAK group. 4. The expression of SYP and PSD95 was the lowest in the Epilepsy group, with a statistically significant difference. 5 The number of synapses in the hippocampus was significantly lower in the Epilepsy group, Epilepsy group and Control group, and the number of synapses in the EP + BAY and EP + TAK group was significantly different. 6. In the Morris water maze test, compared with the EP, the EP+BAY and EP+TAK groups exhibited reduced escape latency, increased of platform crossings, and increased time spent in the target quadrant. Conclusion: This study proved at the organization level that in epilepsy, the TLR4/NF-κB inflammatory pathway is inhibited, which decreases the expression of chemokine receptor CX3CR1, promotes the expression of presynaptic membrane proteins and postsynaptic membrane proteins, increases the number of synapses, improves the order of synaptic structures, inhibits microglia via abnormal synaptic pruning and synaptic E/I imbalances, and inhibits the occurrence and severity of epilepsy, thus ameliorating head injury after the onset of epilepsy.
Modelling Loan Classification as a Markov Chain
Authors: Gidigah Pascal , ODOI BENJAMIN 
Published: September 2022

Abstract
This paper examined the loan portfolio classification criteria with the application of discrete time Markov chain. Default Portfolio were classify under three stage; Sub-standard, Doubtful and Loss in R programming. We predicted that Import and export from some specific classes states is possible with certain probabilities and stationary states distribution of 38 percent sub-standard, 33 percent Doubtful and 27 percent loss of a given portfolio at specific time period.
New Type of Aeronautical Risk Assessment: Performance of Kohonen Self-Organizing Maps in Identifying Brazilian Aircraft with Greater Associated Risks
Authors: Silva Marcell Bruno Sousa e 
Published: September 2022

Abstract
The purpose of this paper is to present a new way of assessing aeronautical risk using a configuration of Kohonen Self-Organizing Maps (SOM) to identify the Brazilian aircraft more likely to be involved in aeronautical accidents and the riskiest Brazilian aircraft. The technique described is classified as predictive for managing aeronautical risks, according to DOC 9859, and can be used both in the context of prevention and investigation of aeronautical accidents/incidents, as well as in the context of the insurance industry. Using this technique, it was possible to identify the 147 Brazilian aircraft with the highest associated probabilities of occurrence of aeronautical accidents, and the 180 with the highest associated risks. Five years after this identification, the respective percentages of aeronautical accidents/incidents were 34% and 27%. The application of this technique can help achieve the objective of the aeronautical community in determining what, where, and when the next aeronautical accidents and/or incidents will occur. Another aspect of the present work is to demonstrate that data collected by the national civil aviation agency in Brazil can be used to implement a predictive methodology for the management of safety in civil aviation.
Performance Study of HF communication using NOMA over Narrowband HF Channel
Authors: Ismail Ahmad , Mohamedpour Kamal 
Published: September 2022

Abstract
HF systems are still important for many communication applications. Data can be sent for a long distance with low cost. One major drawback of them is their low bitrate. The aim of this paper is to increase the bit rate per HF narrowband channel using NOMA. Two parts of Data has been divided and sent for one user over two different power levels. In the receiver side these two parts Data are reorganized using NOMA technique. The results review that using NOMA in a Narrowband 3 kHz channels is reliable. Compared to the maximum rate proofed in previous works using conventional methods, the proposed method increases the rate by 45%. Combined with multichannel algorithms such as HF XL, the total increase in bit rate can be considerable. Results are validated with real experiments. The system is built on SDR plate form and tested in different channel conditions.
Effect of stocking density and vitamin E or zinc supplementation on growth, physiology, gene expression, and economic efficiency of growing broiler chicks
Authors: Shehata Seham F , Baloza Samar H , Elsokary Mohamed M.M. , Hashem Nesrein M. , Khawanda Maha M 
Published: September 2022

Abstract
A total of 636 one-day-old Cobb chicks were randomly assigned to seven treatments. The chicks were offered feed and water ad libitum throughout the experimental period. The first three groups included different stocking densities of broiler birds (low stocking density, LSD: 10 birds/m2; medium stocking density, MSD: 15 birds/m2; and high stocking density HSD: 17 birds/m2). The LSD group was considered a control group. The other four groups included MSD or HSD broiler birds supplemented with either Vit E (100 mg/kg DM diet; MSDVE and HSDVE) or Zn (100 mg/kg DM diet; MSDZn and HSDZn) in their basal diet. The main findings indicated that HSD and MSD negatively affected (p < 0.05) all variables under investigation compared with LSD. Compared with LSD, broiler birds in the MSD and HSD groups had lower body weights and higher feed conversion ratios, higher co ncentrations of blood plasma hormones (triiodothyronine thyroxine and corticosterone), and downregulated expression levels of hepatic growth hormone and insulin-like growth factor-l. In addition, broiler birds stocked at medium or high densities resulted in less economic return and profit. Vit E or Zn supplementation to broiler birds stocked at medium or high densities significantly reversed all adverse effects of HSD (>10 birds/m2) on growth performance, hormones, and gene expression. It could be recommended that adding Zn at a level of 100 mg/kg per DM diet allows increasing the stocking density of broiler birds from 10 birds/m2 to 15 birds/m2 while maintaining the birds, welfare, and economic profit.
Social worlds of attitudes towards anti-COVID-19 vaccination: a Multi-Sited Approach to contextualise a Society in Europe
Authors: Sandu Dumitru 
Published: September 2022

Abstract
What does the balance of attitudes towards the anti-COVID-19 vaccination look like in an eastern European Society? We compared what the Romanians in Romania, interviewed on the subject in the Flash Eurobarometer 494, say with what those from other European Union countries said. From the "discussion with the data" we understood that there are not only ani-vaxxers, pro-vaxxers, and hesitant. There are other categories that matter, between them. We tried to see how much it mattered, in the pro- or anti-vaccination beliefs, not only the usual demographic conditionings of education, age, gender, or residential environment, but also personal pre-pandemic vaccination experiences, as an adult, or trust in institutions relevant to the COVID-19 vaccination. How is the typical Romanian compared to the typical European from the perspective of the main factors of conditioning the attitude towards the anti-COVID-19 vaccination?
Exosomes from high glucose-treated macrophages promote epithelial–mesenchymal transition of renal tubular epithelial cells via long non-coding RNAs
Authors: Yang Huayu , Bai Yu , Fu Chen , Liu Wenhu , Diao Zongli 
Published: September 2022

Abstract
Background: Macrophages contribute to epithelial–mesenchymal transition (EMT) in diabetic nephropathy (DN). Exosomal long non-coding RNAs (lncRNAs) derived from macrophages play a major role in transmitting biological information, whereas related studies on DN are rare. Here we investigated the effects of exosomal lncRNAs from high glucose-treated macrophages on EMT. Methods: High glucose-treated macrophage exosomes (HG-exos) were extracted by coprecipitation and stabilized. Then, mouse renal tubular epithelial cells were treated with HG-exos for 24 hours. Expression of E-cadherin, α-smooth muscle actin (α-SMA), and fibronectin was detected by western blotting, qPCR, and immunofluorescence. High-throughput sequencing was then applied to analyze the bioinformatics of HG-exos. Results: HG-exos inhibited the proliferation of tubular epithelial cells. Additionally, HG-exos markedly upregulated α-SMA and fibronectin expression and downregulated E-cadherin expression in tubular epithelial cells, indicating EMT induction. A total of 378 differentially expressed lncRNAs and 674 differentially expressed mRNAs were identified by high-throughput sequencing of HG-exos. Bioinformatics analysis and subsequent qPCR validation suggested 23 lncRNAs were enriched in the EMT-related MAPK pathway. Among them, ENSMUST00000181751.1, XR_001778608.1, and XR_880236.2 showed high homology with humans. Conclusion: ﻿Exosomes from macrophages induce EMT in DN and lncRNAs in exosomes enriched in the MAPK signaling pathway may be the possible mechanism.
Torsional instability in suspension bridges: a simplified nonlinear model
Authors: ZURRU MARCO 
Published: September 2022

Abstract
A simplified nonlinear model for the dynamic behaviour of suspension bridges is proposed, based on sinusoidal shape functions. The model is applied to the understanding of torsional instability. Numerical experiments are performed using the structural parameters of the Tacoma Narrows Bridge, with initial conditions similar to those observed before the collapse. Each simulation spans about 30 minutes. In line with previous work, numerical results show how torsional instability depends on total energy, with a specific energy level required for each set of initial conditions to reach instability. The time before the onset of instability varies for each initial condition. Interestingly, the model indicates a lack of energy transfer to non-initially-excited even modes. The present numerical investigation confirms recent results, while the proposed model provides a simple and intuitive tool to simulate the relationship between design parameters and potential instability phenomena.
SIRT6-PAI-1 axis is a promising therapeutic target in aging-related bone metabolic disruption
Authors: Aobulikasimu Alkebaier , Tao Liu , Piao Jinying , Sato Shingo , Ochi Hiroki , Okawa Atsushi , Tsuji Kunikazu , Asou Yoshinori 
Published: September 2022

Abstract
The mechanistic regulation of bone mass in aged animals is poorly understood. In this study, we examined the role of SIRT6, a longevity-associated factor, in osteocytes, using mice lacking Sirt6 in Dmp-1-expressing cells (cKO mice) and the MLO-Y4 osteocyte-like cell line. cKO mice exhibited increased osteocytic expression of Sost, Fgf23 and senescence inducing gene Pai-1 and the senescence markers p16 and Il-6, decreased serum phosphate levels, and low-turnover osteopenia. The cKO phenotype was reversed in mice that were a cross of PAI-1-null mice with cKO mice. Furthermore, senescence induction in MLO-Y4 cells increased the Fgf23 and Sost mRNA expression. Sirt6 knockout and senescence induction increased HIF-1α binding to the Fgf23 enhancer sequence. Bone mass and serum phosphate levels were higher in PAI-1-null aged mice than in wild-type mice. Therefore, SIRT6 agonists or PAI-1 inhibitors may be promising therapeutic options for aging-related bone metabolism disruptions.
“Pain Neuroscience Education as an Adjunct to Physiotherapy Protocol in Knee Osteoartritis”- A Case Report
Authors: Walimbe Vaibhavi , Kulkarni Yash , Deolankar Apurva , Chitapure Tajuddin 
Published: September 2022

Abstract
Background: Knee Osteoarthritis affects the entire joint, causing synovial inflammation, cartilage injury, bone remodeling, and the development of osteophytes. Pain, muscle fatigue, joint dysfunction, brief morning stiffness, crepitus, and functional limitations are the common symptoms of knee osteoarthritis.Pain Neuro Science Education (PNE) produces wonderful effects in altering the pain perception of patients by changing their perspective towards pain and reducing pain catastrophization.Methodology A 61 year old female primarily complaining of pain in her right knee (9 on NPRS) along with crepitus, swelling and painful knee flexionfor which shereceived conventional physiotherapy treatment (Cryotherapy, IFT, Ultrasound, strengthening exercises) along with pain neuroscience education PNE (Neurobiology of pain, Education about the pathology of OA, Audio visual aids 30 min session for 5 days Outcome measures were NPRS for pain, pain catastrophizing scale (PCS).Result: PNE has/was shown an additive effect on overall reducing pain and pain catastrophizing along with the conventional physiotherapy treatment.
Outcome of Percutaneous coronary intervention and associated factors among patients with coronary artery disease in selected public hospital Addis Ababa, Ethiopia, 2022; A cross-sectional study
Authors: Amdestion Niguse , Fetensa Getahun , Wondimu Mestawot , Bires Abebaw , Lameesa Fayisa , Haile Biruk 
Published: September 2022

Abstract
Background
Percutaneous coronary intervention is a non-surgical, invasive revascularization procedure of relieving the narrowing or occlusion of the coronary artery and improving blood supply to the ischemic tissue. However, in Ethiopia, limited numbers of research regarding outcomes of percutaneous coronary intervention, and most of them lack representativeness.
Objectives
The aim of this study is to assess the outcome of percutaneous coronary intervention and associated factors among patients with coronary artery disease in selected public hospitals in Addis Ababa.
Methods
Institutional-based cross-sectional study was conducted from January 2017 up to September 2021 GC in the coronary unit of the selected public and private hospitals. Records of patients from the coronary cardiac unit (n = 408) were reviewed and the sample size was allocated proportionally and subjects were selected by using a simple random sampling technique. The data were abstracted using a checklist and entered into Epi Data manager version 4.6 and statistical analyses were performed using the SPSS version 25. The strength of association was assessed using odds ratio and Variables that had a value of P ≤ 0.25 on bivariable analysis were directly forward to be analyzed by multivariable analysis then having P-values < 0.05 is considered as statistically significant.
Result
Out of 422 charts,408 were reviewed with a response rate of 96%, prevalence of procedure-related complications was 18.4%. Being male (AOR = 6.5295%CI:2.41–17.66), diabetes mellites patients (AOR = 2.1795%CI:1.17–4.03), being chronic kidney disease patient (AOR = 3.53, 95%CI:1.37–9.08), having a history of smoking (AOR = 3.69 95%CI:1.77–7.68), radial access (AOR = 0.38,95%CI:0.15–0.99), having ejection fraction of < 30%(AOR = 3.8595%CI:1.47–10.08), from31-40%(AOR = 3.68,95%CI:1.56–8.69) respectively were significantly associated with the outcome of percutaneous coronary intervention.
Conclusion
This study shows that percutaneous coronary intervention in spite of a low mortality rate, complications remain a concern. It is crucial to conduct further studies to identify other keys to determine percutaneous coronary intervention that might be better to conduct a prospective study with better investigation tools.
Multi-objective pathfinder algorithm for multi-objective optimal power flow problem with random renewable energy sources: wind, photovoltaic and tidal
Authors: Li Ning , Zhou Guo , Zhou Yongquan , Deng Wu , Luo Qifang 
Published: September 2022

Abstract
Classical optimal power flow problem is an important optimization problem of power system. Renewable energy can produce electricity with near zero pollution, with the increasing popularity of renewable energy, it becomes more and more urgent to study the optimal power flow problem with renewable energy. In this paper, the standard IEEE30 bus is modified to incorporate renewable energy, and the multi-objective optimal power flow (MOOPF) problem is studied. The MOOPF problem optimization objectives include generation cost, emission, real power loss and voltage deviation (VD). Three renewable energy sources with successful industrial applications including wind energy, solar energy and tidal energy been introduced. Weibull distribution probability, lognormal probability and Gumbel probability are used to calculate the instability and intermittency of wind energy, solar energy and tidal energy, respectively. In order to solve the multi-objective optimal power flow problem with multiple renewable energy sources, a named multi-objective pathfinder algorithm (MOPFA) based on elite dominated set and crowding distance is proposed. Simulation results show that MOPFA can get more evenly distributed Pareto front and provide more diverse solutions. A Compromise solution was selected by the fuzzy decision system. The compromise solution obtained by MOPFA can effectively dispatch the power distribution of the system, and is more inclined to use renewable energy, reducing the power used by thermal power units, which can significantly minimize emissions and other optimization objective. Comparison with the recently published literature also shows that the proposed model can effectively reduce emission and other indicator. In addition, the statistical test results show that MOPFA's multi-objective optimization performance ranks first.
Apolipoprotein C3 facilitates internalization of cationic lipid nanoparticles into terminally differentiated bone marrow-derived mouse mast cells
Authors: Alam Syed Benazir , Wang Feng , Qian Hui , Kulka Marianna 
Published: September 2022

Abstract
Mast cells (MCs), are hematopoetically-derived secretory immune cells that release preformed as well as de novo synthesized inflammatory mediators in response to activation by several stimuli. Based on their role in inflammatory responses, particularly in the lung and skin, MCs provide a convenient and effective target for anti-inflammatory therapeutic strategies. Drug-delivery of lipophilic payloads to MCs can be challenging due to their functionally distinct intracellular structures. In the present study, pH-sensitive cationic lipid-based nanoparticles (LNPs) composed of DODMA, DODAP or DOTAP lipids that encapsulated a GFP or eGFP plasmid were constructed using non-turbulent microfluidic mixing. This approach achieved up to 75-92% encapsulation efficiency. Dynamic light scattering revealed a uniform and homogeneous size distribution of LNPs.To promote cellular internalization, LNPs were complexed with apolipoproteins, amphipathic proteins capable of binding lipids and facilitating their transport in the bloodstream and into cells. Cryo-TEM analysis showed that LNP structure was differentially modified when associated with different types of apolipoproteins. LNP preparations made up of DODMA or DODMA, DODAP and DOTAP lipids were coated with seven apolipoproteins (Apo A1, B, C3, D, E2, E4 and H). Terminally differentiated bone-marrow derived mouse mast cells (BMMCs) were exposed to Apolipoprotein-LNP mixture and internalization was measured using flow cytometry. Out of all the apolipoproteins tested, ApoC3 most efficiently facilitated cellular internalization of the LNP into BMMCs as determined by GFP fluorescence using flow cytometry. These effects were confirmed in a less differentiated but also interleukin-3-dependent model of mouse mast cells, MC/9. ApoC3-LNP enhanced internalization by BMMC in a concentration-dependent manner and this was significantly increased when BMMC were pre-treated with inhibitors of actin polymerization, suggesting a dependence on intracellular shuttling. Activation of peroxisome proliferator-activated receptor gamma (PPAR) decreased ApoC3-LNP internalization and reduced the expression of apolipoprotein E receptor 2 (ApoER2) , suggesting that ApoC3-LNP binding to ApoER2 may be responsible for its enhanced internalization. Altogether, our studies reveal an important role of ApoC3 in facilitating internalization of cationic LNPs into MCs.
Single-cell analysis of mast cell populations across organs reveals insights into cell origins, anatomical niches and neuron-associated functions.
Authors: Gaudenzio Nicolas , Tauber Marie , Basso Lilian , Wemelle Eve , Martin Jeremy , Bostan Luciana , Pinto Marlene Magalhaes , Thierry Guilhem , Houmadi Raissa , Serhan Nadine , Loste Alexia , Bleriot Camille , Samrout Celine El , Lasrado Reena , Kamphuis Jasper , Pachnis Vassilis , Grujic Mirjana , Kjellén Lena , Pejler Gunnar , Paul Carle , Dong Xinzhong , Galli Stephen , Reber Laurent , Ginhoux Florent , Bajenoff Marc , Gentek Rebecca , Knauff Claude 
Published: September 2022

Abstract
Mast cells (MCs) are heterogenous tissue-resident immune cells associated with blood vessels and neurons. However, the extent to which specialized MCs can exhibit micro-environment-specific functions is unclear. Using single cell RNA sequencing, we characterize connective tissue-type and mucosal MCs across organs and found that they can be discriminated based on MrgprB2 expression. While MrgprB2+ connective MCs are enriched in neuroreceptors, develop during the embryogenesis and renew independently of the bone marrow (BM); MrgprB2neg mucosal MCs arise postnatally, are sensitive to signals from the microbiome and renewed by BM progenitors. Importantly, MrgprB2+, but not MrgprB2neg MCs are required for food-induced anaphylactic shock. In the gut, MrgprB2+ MCs occur in contact with substance P-secreting enteric neurons and the specific depletion of either MrgprB2+ MCs or substance P results in dysregulation of gut contractions. Thus, we demonstrate that two independent MC populations exist in multiple organs, with different developmental dynamics and biological programming.