bluuebunny/crossref_metadata_2025_split huggingface dataset:
G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and glycolysis modulation
Authors: Yuan Lifang , Hang Weiyu , Bi Yin , Chen Saiqiong , Wang Xi , Li Ting , Wei Peiru , Du Jiebing , Zhao Ling , Liu Bo , Yang Yihua 
Published: January 2022

Abstract
Background: The ovarian function restoration of premature ovarian insufficiency (POI) patients is one of the most challenging problems in the reproduction field, as no evidence-based efficient therapy exists. Regenerative medicine with peripheral blood mononuclear cells (PBMCs) transplantation sheds light on this issue. However, the efficiency and safety of PBMCs treatment in patients with natural ovarian aging (NOA) remain unclear.Methods: To investigate the effect of PBMCs alone or combined with PRP on NOA functional recovery, we used 13-month-old female Sprague–Dawley (SD) rats as an NOA model. Seventy-two NOA rats were randomly divided into three groups: the NOA control group, PBMCs group, and PBMCs combined with platelet-rich plasma (PRP) group. PBMCs and PRP were transplanted by intraovarian injection. The effects of ovarian function and fertility ability were measured and compared among different groups after transplantation.Results: Intraovarian transplantation of PBMCs alone or in combination with PRP resulted in restoration of the normal estrus cycle, consistent with recovery of serum sex hormone levels, increased follicle numbers at all stages, and restoration of fertility by facilitating pregnancy and live birth. Moreover, when combined with PRP injection, these effects were more significant. The male-specific SRY gene was detected in the ovary at all four time points, which suggested that PBMCs continuously survived and functioned in NOA rats. In addition, after PBMCs treatment, the expression of the angiogenesis-related marker VEGF and glycolysis-related molecules lactate dehydrogenase A (LDH-A) and sirtuin 1 (SIRT1) in NOA-overexpressing cells was upregulated, which indicated that these effects were associated with angiogenesis and glycolysis.Conclusions: PBMCs transplantation can restore the ovarian functions and fertility of NOA rats by increasing ovarian vascularization, folliculogenesis and glycolysis, and PRP could enhance the efficiency. However, more studies are required to evaluate the feasibility, safety, and efficacy of these novel fertility-preserving methods.
The Prevalence of Cardiovascular Risk Factors in Patients with and Without Metabolic Syndrome in Diabetes Mellitus: A Study Based on Rafsanjan Cohort Study
Authors: Kamiab Zahra , Abbasifard Mitra , Nadimi Ali Esmaeili , Alinejad Hasan , Bazmandegan Gholamreza 
Published: January 2022

Abstract
Background
Cardiovascular disease (CVD) is the leading causes of death and disability in diabetes. This study aimed to determine the prevalence of cardiovascular risk factors in people with and without metabolic syndrome (MtS) in diabetes mellitus (DM).
Methods
This cross-sectional study was part of Rafsanjan Cohort Study (RCS).as part of the comprehensive PERSIAN (Prospective Epidemiological Research Studies in IrAN) on adults with and without MtS in DM. CVD risk factors, including gender, age, blood pressure, dyslipidemia, smoking, alcohol consumption, fasting blood sugar, creatinine, blood urea, waist circumference, body mass index, family history, physical inactivity, fruit and vegetable consumption were collected in the PERSIAN Cohort Questionnaire. The data were analyzed by SPSS software version 22.
Results
The prevalence of MtS in 1933 participants was estimated to be 80% (95% confidence interval 81.8% -78.1%). In the logistic regression model, smoking, alcohol consumption, triglyceride to HDL (High Density Lipoprotein) ratio, abdominal obesity, and hypertension were identified as the factors associated with MtS.
Conclusions
Our results show that Based on our study, the prevalence of cardiovascular risk factors in DM was high. Reducing smoking and alcohol consumption and controlling hypertension, hyperlipidemia, and overweight are the suggested solutions in this field.
Reactive strength index during single-leg vertical continuous jumps after anterior cruciate ligament reconstruction: cross-sectional study
Authors: Hirohata Kenji , Aizawa Junya , Ohmi Takehiro , Ohji Shunsuke , Mitomo Sho , Ohara Toshiyuki , Koga Hideyuki , Yagishita Kazuyoshi , Jinno Tetsuya , Okawa Atsushi 
Published: January 2022

Abstract
Background

The association of the reactive strength index (RSI) during single-leg vertical continuous jumps (SVCJs) with single-leg hop tests in athletes after anterior cruciate ligament reconstruction (ACLR) is unclear. Thus, this study aimed to confirm the measurement properties of the RSI during SVCJs in athletes with ACLR at the phase of determining the timing of their return to sport.
Methods

RSI during SVCJs and single-leg hop (single, triple, and crossover) tests were measured for post-ACLR and healthy athletes. The limb symmetry index (LSI) was calculated using the measurements of each parameter. For each test, patients were divided into two subgroups according to their LSI score (≥90%, satisfactory; <90%, unsatisfactory). Fisher’s exact test was used to examine the association of single-leg hop tests with RSI during the SVCJs.
Results

A total of 21 post-ACLR and 17 healthy athletes completed all the tests. RSI during SVCJs were significantly lower on the involved limb than on the uninvolved limb in post-ACLR athletes (P < 0.001). The LSI of RSI during SVCJs of post-ACLR athletes were significantly lower than that of the healthy athletes (P < 0.01). Among the post-ACLR athletes, <30% of those with LSIs >90% in the single-leg hop tests had an LSI >90% of the RSI during SVCJs.
Conclusions

RSI during SVCJs of post-ACLR athletes was significantly lower on the involved limb than on the uninvolved limb, and the asymmetry was more remarkable in the SVCJs than in the single-leg hop tests.
Analysis of the Risk Factors of Soft Tissue Bleeding Phenomenon in Covid-19 Patients: the Point of View After Two Years of the Pandemic.
Authors: Faiella Eliodoro , Castiello Gennaro , Santucci Domiziana , Altomare Carlo , Pacella Giuseppina , Bernetti Caterina , Ferrari Ugo , Villamu Moises Muley , Incalzi Raffaele Antonelli , Zobel Bruno Beomonte , Grasso Rosario Francesco 
Published: January 2022

Abstract
Background: to analyze the relationship between patient characteristics, including anagraphic, laboratoristic data and amount of adipose tissue measured in Computed Tomography (CT)-scans in COVID-19 patients, and incidence of soft-tissue bleeding requiring medical and/or interventional radiology management. A total of 132 patients hospitalized for COVID-19 pathology from October 2020 to May 2021, were included in the study, considering two groups: a bleeding group, 70 cases of soft-tissue bleeding occurred during hospitalization, and a control group, 62 hospitalized COVID-19 patients without bleeding events. In the bleeding group, two subgroups were considered: an embolization group including soft tissue bleeding cases requiring interventional radiology TAE (Transarterial Embolization) (16/70; 22.9%) and a non-embolization group, clinically managed without TAE (54/70; 77.1%). Demographics and clinical data, visceral adipose tissue (VAT), sub-cutaneous adipose tissue (SAT) areas measured on CT images and VAT/SAT ratio were compared between bleeding and control groups and between embolization and non-embolization subgroups. Results: Bleeding and control groups did not significantly differ for sex distribution, Covid-19, platelet count, SAT-area, VAT-area and VAT/SAT ratio. Embolization and non-embolization groups did not significantly differ for age, Covid-19, platelet count, SAT area, and VAT/SAT ratio. A statistically significant difference was observed between embolization and non-embolization groups for VAT area, with smaller values in embolization group (mean difference: 64.2 cm², 95% CI, 8.3 – 120.1; p<0.05). Conclusions: Soft-tissue bleeding in COVID-19 is more frequent and severe in patients with low amount of visceral adipose tissue, demonstrating that fat mass may have a containing function on bleeding, limiting its progression in surrounding structures. Other factors influence the risk of bleeding, such as age.
Identifying the functional pathways and potential genes associated with interferon signaling during human adenovirus type 7 (HAdV-7) by weighted gene co-expression network analysis
Authors: Yang Zhongying , Wei Jianhua , He Yu , Ren Luo , Chen Shiyi , Deng Yu , Zang Na , Liu Enmei 
Published: January 2022

Abstract
Background
Human adenovirus type 7 (HAdV-7) can cause severe pneumonia in children and severe complications. At present, however, the pathogenesis and genes involved remain largely unknown.
Methods
We collected HAdV-7 infected and mock-infected A549 cells at 24, 48, and 72 hours post-infection (hpi) for RNA sequencing (RNA-seq). And, we identified potential genes and functional pathways associated with HAdV-7 infection using weighted gene co-expression network analysis (WGCNA).
Results
Based on bioinformatics analysis, 12 co-expression modules were constructed by WGCNA, with the blue, tan, and brown modules significantly positively correlated with Ad-24 hpi, Ad-48 hpi, and Ad-72 hpi, respectively. Functional enrichment analysis indicated that the blue module was mainly enriched in DNA replication, cell cycle, and viral process; the tan module was largely enriched in metabolic pathways and regulation of superoxide radical removal; and the brown module was predominantly enriched in mitochondrial respiratory chains and regulation of cell death. Quantitative polymerase chain reaction (qPCR) was used to determine transcript abundance of some identified hub genes, and results were consistent with those from RNA-seq. Based on comprehensive analysis of hub genes and differentially expressed genes (DEGs) in the GSE68004 dataset, we identified SOCS3, OASL, ISG15, and IFIT1 as potential candidate genes for biomarkers or drug targeting in HAdV-7 infection. Further, we propose a multi-target inhibition of interferon signaling mechanism to explain the HAdV-7 associated with higher severity of clinical consequences.
Conclusions
We constructed a framework of co-expression gene modules from A549 cells infected with HAdV-7, thus providing a basis for identifying potential genes and pathways involved in adenovirus infection and for studying the pathogenesis of adenoviruses.
Critical Observations on the Dynamic Change in Caliber of Interarterial Segment of Anomalous Coronary Arteries on CT Angiogram in Patients with Chest Pain and Treadmill Test Findings - A Potential ‘Game-Changer’ Information in the Patient Management
Authors: Mahalingam Vijay , Gadabanahalli Karthik , Bhat Venkatraman 
Published: January 2022

Abstract
Aims and backgroundThe incidence of coronary artery anomalies is rare in the general population, anomalous origin of right coronary artery being the most common. These anomalies, particularly anomalous coronary arteries with an interarterial course (ACAIAC) are potentially dangerous. Due to their low incidence, meticulous clinical and imaging guidelines have not yet been defined in assessing such patients and guiding management. Methods and resultsCT coronary angiograms of patients who underwent the study for exclusion of coronary artery disease were reviewed. Patients with ACAIAC were recorded. The images were reviewed and reconstructed to measure the caliber and area of the narrowest interarterial segment of ACAIAC in systolic and diastolic phases using Philips Intellispace version 12.1 software.Percentage change in area (p value 0.093) and diameter (p value 0.108) of the interarterial segment in systolic and diastolic segments, was statistically significant between anomalous coronaries with high and low interarterial course. Percentage change in area and diameter between patients with positive and negative TMT findings was also statistically significant (p<0.001 in both cases). ConclusionSignificant positive correlation between change in vessel caliber in the interarterial course of coronary arteries during the cardiac cycle and TMT findings, suggests elevated risk of inducible ischemia in patients with significant vessel compression. Hence the change in vessel caliber can be used as a potential criterion for risk assessment and management of patients with ACAIAC.
A Sclerosing Stromal Tumour of the Ovary in a postmenopausal Female: A Rare Case Report
Authors: Saraf Pallavi , BHARTI JYOTSNA NARESH , Sharma Charu 
Published: January 2022

Abstract
Sclerosing stromal tumour (SST) is an extremely rare benign ovarian neoplasm, usually seen in young females. We report a case of a 55-year-old female who presented with pelvic pain and irregular bleeding. On imaging, she was suspected of have a borderline malignant tumour of the left ovary and benign teratoma of the right ovary. Postoperatively, diagnosis of SST of left ovary and mature cystic teratoma of right ovary was made after histopathological examination and immunohistochemistry. Our case is unique because SST is a rare entity, and its existence with teratoma has not been reported to date in the literature; and lastly, its occurrence in elderly females is also rarer.
Cost-efficient multiplex PCR-based metabarcoding with new comprehensive primer sets for monitoring plankton diversity dynamics
Authors: Zou Shanmei , Smith Lydia 
Published: January 2022

Abstract
Aim: Accurate and quick monitoring of plankton diversity dynamics is critical and urgent for aquatic environmental protection, especially for bloom management. Multiple genetic markers with efficient primers should be considered for accurate species detection by metabarcoding. Here we aim to provide cost-efficient multiplex PCR-based metabarcoding (CMPM) with new comprehensive primer sets for monitoring plankton diversity dynamics. The CMPM assay was tested in samples from Lake Tai.Location: Freshwater Lake Tai, ChinaMethods: A total of nine primer sets from 18S, 28S, 23S, 16S and rbcL was presented, including five newly designed ones 18S-v4-5, 28S, 16S-new, rbcL-Chlo and rbcL-Cry in this study. Three multiplex PCR-based system were developed for Next Generation Sequencing of all nine genetic markers. The assignment efficiency of new primer sets for plankton were detected. The taxonomic assignments of all genetic markers were compared in each of species, genus and family level. The single and multiplex PCR-based metabarcoding were compared. The assignments from multiple genetic markers and microscope observations were compared in the reference samples. The CMPM assay was tested in samples from Lake Tai. Results: The taxonomic assignments of all nine genetic markers differed from each other in each of species, genus, family and phylum level. The 28S identified a higher proportion of phytoplankton, zooplankton and fungi with high assignment performance in species level. The 16S-new marker not only obtained large cyanobacteria assignments, but also obtained other assignments from phytoplankton and bacteria. The new 18S-v4-5 was much variant to detect a broader range of zooplankton and phytoplankton. The two new primer sets of rbcL-Chlo and rbcL-Cry specifically assigned Chlorophyta and Cryptophyta successfully. The multiple genetic markers produced more plankton assignments than microscope observations. The plankton communities in Lake Tai showed pronounced seasonal dynamic by CMPM, which is consistent with the previous reports. Main conclusions: The 28S, 16S-new and 18S-v4-5 newlywed designed here could be as complementary universal genetic markers for identifying plankton. The rbcL-Chlo and rbcL-Cry could be as specific markers for identifying Chlorophyta and Cryptophyta respectively. The CMPM assay can provide precise identification and save cost for monitoring plankton diversity dynamics with comprehensive primer sets.
Matrix Metalloproteinases; A Biomarker of Disease Activity and Prognosis
in Spondyloarthritis: A Narrative Review
Authors: Rahmouni Safa , Slouma Maroua , Bouzid Sirine , Dhahri Rim , Litaiem Noureddine , Gharsallah Imen , Metoui Leila , Louzir Bassem 
Published: January 2022

Background:
Matrix metalloproteinases, as components of the proteolytic system, are
deemed to be implicated in the pathogenesis and progression of several rheumatic diseases. Their role
in spondyloarthritis has been investigated by several studies.

Objective:
This article aims to review and summarize the current knowledge related to metalloproteinases
in patients with spondyloarthritis.

Methods:
To examine the association between matrix metalloproteinases and spondyloarthritis, we
conducted a narrative review using a literature search in SCOPUS for English-language sources. The
search included studies published from the database inception to December 2020.

Results:
A total number of 74 articles were included. It was found that levels of matrix metalloproteinases
3 were higher in radiographic axial spondyloarthritis patients and seemed to play a role in the
progression of joint damage. The levels of matrix metalloproteinases 1, 2, and 9 were upregulated in
psoriatic arthritis patients compared to psoriasis and could identify psoriasis patients who would develop
rheumatic manifestations. The levels of matrix metalloproteinases correlated significantly with
disease activity in ankylosing spondylitis and decreased upon treatment with Tumor Necrosis Factor
inhibitors (TNFi).

Conclusion:
Excessive matrix metalloproteinases activity is associated with articular destruction.
Their levels can reflect disease activity, structural damage, and response to TNFi in patients with
spondyloarthritis. Nevertheless, further studies are needed to confirm these results.
Reparación Directa y Conflicto Armado Interno. La Constitucionalización de la Responsabilidad Extracontractual del Estado
Authors: Roncancio-B. Andrés Felipe , Restrepo-M. Mariana , Hoyos-García José Fernando 
Published: January 2022

Introducción: En materia de responsabilidad extracontractual del Estado, los procesos de reparación directa que se han derivado de hechos con ocasión al Conflicto Armado Interno, en adelante -CAI- han generado cambios en las categorías de protección que se han ido transformando especialmente desde que se reconoce la existencia del CAI. Objetivo: el principal objetivo de esta investigación está articulado a determinar cuáles son las condiciones que se han valorado por parte del Consejo de Estado para establecer la adecuación frente a los títulos de imputación y asimismo, las condiciones de interpretación de las categorías del daño antijuridico y la responsabilidad del Estado. Materiales y Métodos: El estudio fue delimitado a partir de la revisión documental de una elección aleatoria de sentencias del Consejo de Estado tomadas de forma no probabilística a través de las categorías de los títulos de imputación que se presentaron en una reconstrucción cronológica donde el eje referencia se contrastó en una dimensión analítica del reconocimiento del CAI. Resultados: el estudio evidenció cómo entre los diferentes títulos de imputación aplicables a hechos del CAI existen parámetros que evidencian una transición de omisión institucional que permitía un margen de la concepción del daño antijurídico en un sentido amplio, hacia un margen más limitado fundamentado en un desarrollo más riguroso del principio de legalidad. Conclusiones: La declaración del CAI tuvo una gran incidencia en la forma como se desarrolló la responsabilidad extracontractual del Estado, que al disponer de nuevas categorías de protección generó con ello más seguridad jurídica, pero, que a su vez limitó interpretaciones amplias del daño antijurídico.
Psychological Well-Being amongst Cancer Palliative Care Professionals working in Bengaluru, India
Authors: Kaur Amanpreet , Sharma Mahendra P. , Chaturvedi Santosh K. 
Published: January 2022

Objectives:
Increased levels of psychological distress and burnout in cancer palliative care professionals have implications on their psychological well-being, quality of patient care they provide and for their employing organisations. There is a dearth of studies on psychological well-being with no published study available on psychological well-being amongst cancer palliative care professionals in India. The aim of the present study was to assess psychological well-being amongst cancer palliative care professionals.

Materials and Methods:
The study was cross-sectional and quantitative which was carried out at four cancer palliative care centers (one hospice and three hospitals) in Bengaluru city of India. The tools sociodemographic and professional datasheet and psychological well-being (PWB-20) scale were administered with 65 participants (Mean Age = 32.5, SD = 11.78). Purposive sampling method was used to recruit the participants working full-time at respective centers after obtaining permissions and ethical approvals. Descriptive, correlational, and inferential analysis of the quantitative data was carried out based on normality of the distribution.

Results:
The results revealed above average levels of self-acceptance and engagement and growth, below average levels of mastery and competence, while average levels of positive relations and PWB (total score). Significant differences in PWB domains based on age (P < 0.05) and self-care practices (P < 0.05) were seen. Sense of engagement and growth was found to be positively correlated with age and income earned per month (P = 0.01).

Conclusion:
Findings from the present study suggest that cancer palliative care professionals had moderate levels of PWB with implications in training and future research.
Hemorrhagic brain metastasis from small-cell carcinoma of the urinary bladder
Authors: Maeshima Kazuhide , Sasaki Takahiro , Yamoto Toshikazu , Fukai Junya , Nishibayashi Hiroki , Nakao Naoyuki 
Published: January 2022

Background:
Small-cell carcinoma of the urinary bladder (SCCB) accounts for 1% of all bladder tumors. We present a rare case of hemorrhagic metastatic brain tumor from SCCB diagnosed by navigation-guided endoscopic biopsy.

Case Description:
A 76-year-old man presented with sudden onset of aphasia and right hemiplegia from 3 weeks previously. He had a medical history of prostate cancer and SCCB. Computed tomography showed a mixed density mass in the left basal ganglia. On magnetic resonance imaging, the mass showed mixed intensity in both T1-weighted images and T2-weighted images, suggesting subacute hemorrhage. The mass was partially enhanced with gadolinium. The patient underwent endoscopic hematoma evacuation and partial removal of the tumor. Histopathological diagnosis was neuroendocrine carcinoma, which was consistent with SCCB metastasis. After surgery, the patient underwent whole-brain radiation therapy of 30 Gy. His general condition gradually deteriorated, however, and he died 4 months after surgery.

Conclusion:
Our patient had a rare case of brain metastasis derived from SCCB which presented with cerebral hemorrhage. Navigation-guided endoscopic biopsy was useful for the diagnostic sampling of deep localized brain tumors with hemorrhage.