bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complement C5aR/LPS-induced BDNF and NGF modulation in human dental pulp stem cells
Authors: Irfan Muhammad , Kim Ji Hyun , Druzinsky Robert E. , Ravindran Sriram , Chung Seung 
Published: February 2022

AbstractStem cells with the ability to differentiate into a variety of cells and secrete nerve regeneration factors have become an emerging option in nerve regeneration. Dental pulp stem cells (DPSCs) appear to be a good candidate for nerve regeneration given their accessibility, neural crest origin, and neural repair qualities. We have recently demonstrated that the complement C5a system, which is an important mediator of inflammation and tissue regeneration, is activated by lipoteichoic acid-treated pulp fibroblasts, and governs the production of brain-derived nerve growth factor (BDNF). This BDNF secretion promotes neurite outgrowth towards the injury site. Here, we extend our observation to DPSCs and compare their neurogenic ability to bone marrow-derived mesenchymal stem cells (BM-MSCs) under inflammatory stimulation. Our ELISA and immunostaining data demonstrate that blocking the C5a receptor (C5aR) reduced BDNF production in DPSCs, while treatment with C5aR agonist increased the BDNF expression, which suggests that C5aR has a positive regulatory role in the BDNF modulation of DPSCs. Inflammation induced by lipopolysaccharide (LPS) treatment potentiated this effect and is C5aR dependent. Most important, DPSCs produced significantly higher levels of C5aR-mediated BDNF compared to BM-MSCs. Taken together, our data reveal novel roles for C5aR and inflammation in modulation of BDNF and NGF in DPSCs.
InSAR data reveal that the largest hydraulic fracturing-induced earthquake in Canada, to date, is a slow-slip event
Authors: Eyre Thomas S. , Samsonov Sergey , Feng Wanpeng , Kao Honn , Eaton David W. 
Published: February 2022

AbstractFor tectonic earthquakes, slip rate spans a continuum from creep to supershear earthquakes, where slow slip events (SSEs) are important in releasing stress without radiating damaging seismic energy. Industrial-scale subsurface fluid injection has caused induced earthquakes, but the role of SSEs in fault activation is currently unclear. Ground-deformation observations, measured by satellite radar, show that SSEs up to magnitude 5.0 occurred during hydraulic fracturing (HF) operations in northwestern Canada, corroborated by reported deformation of the steel well casing. Although the magnitude 5.0 SSE exceeded the magnitude of the largest induced earthquake in this region (magnitude 4.55), it was undetected by seismograph networks. The observed SSEs occurred within a buried thrust belt and their magnitude and duration are consistent with scaling behavior of SSEs in unbounded natural systems, e.g. slab interfaces in subduction zones.
Diversity and dynamics of bacteria at the Chrysomya megacephala pupal stage revealed by third-generation sequencing
Authors: Xu Wang , Wang Yu , Wang Ying-hui , Zhang Ya-nan , Wang Jiang-feng 
Published: February 2022

AbstractCharacterization of the microbial community is essential for understanding the symbiotic relationships between microbes and host insects. Chrysomya megacephala is a vital resource, a forensic insect, a pollinator, and a vector for enteric bacteria, protozoa, helminths, and viruses. However, research on its microbial community is incomprehensive, particularly at the pupal stage, which comprises approximately half of the entire larval development stage and is important entomological evidence in forensic medicine. For the first time, this study investigated the bacterial communities of C. megacephala pupae at different ages using third-generation sequencing technology. The results showed that C. megacephala has a diverse and dynamic bacterial community. Cluster analysis at ≥ 97% similarity produced 154 operational taxonomic units (OTUs) that belonged to 10 different phyla and were distributed into 15 classes, 28 orders, 50 families, 88 genera, and 130 species. Overall, the number of bacterial OTUs increased with the development of pupae, and the relative abundance of Wolbachia in the Day5 group was significantly lower than that in the other groups. Within the pupal stage, Proteobacteria, Firmicutes, and Bacteroidetes were the dominant phyla of bacteria. At the genus level, Wolbachia and Ignatzschineria coexisted, a rarely known feature. In addition, we found Erysipelothrix rhusiopathiae, the etiological agent of swine erysipelas, which is rarely identified in insects. This study enriches the understanding of the microbial community of C. megacephala and provides a reference for better utilization and control of C. megacephala.
Retraction Note: Discrimination of urinary exosomes from microvesicles by lipidomics using thin layer liquid chromatography (TLC) coupled with MALDI-TOF mass spectrometry
Authors: Singhto Nilubon , Vinaiphat Arada , Thongboonkerd Visith 
Published: February 2022

AbstractThis Article has been retracted; the Retraction Note is available at: https://doi.org/10.1038/s41598-022-06491-2.
Fgf8 dynamics and critical slowing down may account for the temperature independence of somitogenesis
Authors: Zhang Weiting , Scerbo Pierluigi , Delagrange Marine , Candat Virginie , Mayr Vanessa , Vriz Sophie , Distel Martin , Ducos Bertrand , Bensimon David 
Published: February 2022

AbstractSomitogenesis, the segmentation of the antero-posterior axis in vertebrates, is thought to result from the interactions between a genetic oscillator and a posterior-moving determination wavefront. The segment (somite) size is set by the product of the oscillator period and the velocity of the determination wavefront. Surprisingly, while the segmentation period can vary by a factor three between 20 °C and 32 °C, the somite size is constant. How this temperature independence is achieved is a mystery that we address in this study. Using RT-qPCR we show that the endogenous fgf8 mRNA concentration decreases during somitogenesis and correlates with the exponent of the shrinking pre-somitic mesoderm (PSM) size. As the temperature decreases, the dynamics of fgf8 and many other gene transcripts, as well as the segmentation frequency and the PSM shortening and tail growth rates slows down as T–Tc (with Tc = 14.4 °C). This behavior characteristic of a system near a critical point may account for the temperature independence of somitogenesis in zebrafish.
Geometry-enhanced molecular representation learning for property prediction
Authors: Fang Xiaomin , Liu Lihang , Lei Jieqiong , He Donglong , Zhang Shanzhuo , Zhou Jingbo , Wang Fan , Wu Hua , Wang Haifeng 
Published: February 2022

AbstractEffective molecular representation learning is of great importance to facilitate molecular property prediction. Recent advances for molecular representation learning have shown great promise in applying graph neural networks to model molecules. Moreover, a few recent studies design self-supervised learning methods for molecular representation to address insufficient labelled molecules; however, these self-supervised frameworks treat the molecules as topological graphs without fully utilizing the molecular geometry information. The molecular geometry, also known as the three-dimensional spatial structure of a molecule, is critical for determining molecular properties. To this end, we propose a novel geometry-enhanced molecular representation learning method (GEM). The proposed GEM has a specially designed geometry-based graph neural network architecture as well as several dedicated geometry-level self-supervised learning strategies to learn the molecular geometry knowledge. We compare GEM with various state-of-the-art baselines on different benchmarks and show that it can considerably outperform them all, demonstrating the superiority of the proposed method.
Secretion Bubbling as the Sound Mechanism for Nasal Rustle: A Perceptual Study
Authors: Oren Liran , Kummer Ann W. , Boyce Suzanne 
Published: February 2022

Purpose:
Secretion bubbling on the superior aspect of the velopharyngeal (VP) valve typically occurs with a small VP opening during production of oral pressure consonants. The use of high-speed nasopharyngoscopy has shown correlation between the bubbling frequency and the acoustics captured with the nasal microphone of the nasometer. The purpose of this study was to investigate if the sound generated by the bubbling process is perceived as nasal rustle (also known as nasal turbulence).

Method:
Speech samples were extracted from the data of patients who were diagnosed with nasal rustle (five boys and five girls, ranging in age from 5 to 10 years old). A customized filter was used to remove the sound generated by the secretion bubbling. Six experienced listeners were asked to rate the perception of nasal rustle in each speech stimuli before and after the filtering process.

Results:
Rating values for the perception of nasal rustle were overall reduced in all cases after the filtering process. Furthermore, the perception of nasal rustle was eliminated in 40% of the cases. Rating reliability was excellent before the filtering process and moderate to good after filtering.

Conclusion:
Reducing the perception of nasal rustle using spectral filtering based on the bubbling frequencies supports the hypothesis that undesired sound in the nasal cavity is generated from the interaction of the turbulent airflow with the secretion bubbling.

Supplemental Material:

https://doi.org/10.23641/asha.19111544
A fractional‐N MASH2‐k FDC phase‐locked loop architecture enabling higher‐order quantisation noise shaping
Authors: Iwashita Ryoga , Xu Zule , Osada Masaru , Iizuka Tetsuya 
Published: February 2022

AbstractThe quantisation noise contribution of a conventional FDC phase‐locked loop (PLL) is still high due to the only second‐order noise‐shaping capability. A MASH2‐k FDC PLL architecture enabling (k + 2)th‐order noise shaping for more flexible loop design optimization and for a wider loop bandwidth to suppress the noise from the digitally controlled oscillator is proposed. The linear loop model is derived, and the noise contributions are analysed. The proposed PLL is evaluated with behavioural simulation whose results are consistent with those of the analysis. The results also suggest a lower phase noise of the proposed PLL compared with the conventional one.
Chemistry of nebulae around binary post-AGB stars: A molecular survey of mm-wave lines
Authors: Gallardo Cava I. , Bujarrabal V. , Alcolea J. , Gómez-Garrido M. , Santander-García M. 
Published: February 2022

Context. There is a class of binary post-asymptotic giant branch (post-AGB) stars that exhibit remarkable near-infrared excess. Such stars are surrounded by Keplerian or quasi-Keplerian disks, as well as extended outflows composed of gas escaping from the disk. This class can be subdivided into disk- and outflow-dominated sources, depending on whether it is the disk or the outflow that represents most of the nebular mass, respectively. The chemistry of this type of source has been practically unknown thus far.
Aims. Our objective is to study the molecular content of nebulae around binary post-AGB stars that show disks with Keplerian dynamics, including molecular line intensities, chemistry, and abundances.
Methods. We focused our observations on the 1.3, 2, 3 mm bands of the 30mIRAM telescope and on the 7 and 13 mm bands of the 40 m Yebes telescope. Our observations add up ~600 h of telescope time. We investigated the integrated intensities of pairs of molecular transitions for CO, other molecular species, and IRAS fluxes at 12, 25, and 60 μm. Additionally, we studied isotopic ratios, in particular 17O/18O, to analyze the initial stellar mass, as well as 12CO/13CO, to study the line and abundance ratios.
Results. We present the first single-dish molecular survey of mm-wave lines in nebulae around binary post-AGB stars. We conclude that the molecular content is relatively low in nebulae around binary post-AGB stars, as their molecular lines and abundances are especially weaker compared with AGB stars. This fact is very significant in those sources where the Keplerian disk is the dominant component of the nebula. The study of their chemistry allows us to classify nebulae around AC Her, the Red Rectangle, AI CMi, R Sct, and IRAS 20056+1834 as O-rich, while that of 89 Her is probably C-rich. The calculated abundances of the detected species other than CO are particularly low compared with AGB stars. The initial stellar mass derived from the 17O/18O ratio for the Red Rectangle and 89 Her is compatible with the central total stellar mass derived from previous mm-wave interferometric maps. The very low 12CO/13CO ratios found in binary post-AGB stars reveal a high 13CO abundance compared to AGB and other post-AGB stars.
Silencing LncRNA PVT1 Reverses High Glucose-Induced Regulation of the
High Expression of PVT1 in HRMECs by Targeting miR-128-3p
Authors: Wang Xuyang , Chen Wangling , Lao Wei , Chen Yunxin 
Published: February 2022

AbstractThis paper aims to discuss the possibility of lncRNA PVT1 as a diagnostic
biomarker for diabetic retinopathy (DR) and explore the underlying mechanism.
Real-time quantitative polymerase chain reaction (RT-qPCR) was selected to
determine the expression level of lncRNA PVT1 in the serum of all subjects. The
receiver operating characteristic (ROC) curve reflected the diagnostic
significance of PVT1 for DR patients. The Cell Counting Kit-8 (CCK-8) and
Transwell assays were used to evaluate the effect of PVT1 expression on the
proliferation and migration of human retinal microvascular endothelial cells
(HRMECs). The luciferase reporter gene was selected to verify the interaction
between PVT1 and miR-128-3p. The relative expression level of PVT1 in serum was
higher in both the DB and DR group than in the healthy controls group (HC), and
it was highest in the DR group. ROC curve indicated that serum PVT1 could
distinguish between HC and DB patients, DB patients and DR patients,
respectively. In vitro, high glucose induction significantly increased the
proliferation and migration capabilities of HRMECs, but silencing PVT1 (si-PVT1)
downregulated the proliferation and migration capabilities of HRMECs. The
detection of luciferase reporter gene showed that lncRNA PVT1 targeted
miR-128-3p, and there was a negative correlation in the serum of DR patients. In
conclusion, this study confirmed that lncRNA PVT1 might regulate the process of
DR by targeting miR-128-3p, and has the potential as a biomarker for the
diagnosis of DR.
Surgical Technique and Considerations about Transanal Transection and Single-Stapled (TTSS) Anastomosis: The Search for a Perfect Anastomosis
Authors: Campos Fábio Guilherme , Pandini Rafael Vaz , Bustamante-Lopez Leonardo Alfonso , Nahas Sérgio Carlos 
Published: February 2022

AbstractThe evaluation of preventive measures and risk factors for anastomotic leakage has been a constant concern among colorectal surgeons. In this context, the description of a new way to perform a colorectal, coloanal or ileoanal anastomosis, known as transanal transection and single-stapled (TTSS) anastomosis, deserves an appreciation of its qualities, and a discussion about its properties and technical details. In the present paper, the authors review the most recent efforts aiming to reduce anastomotic dehiscence, and describe the TTSS technique in a patient submitted to laparoscopic total proctocolectomy with ileal pouch-anal anastomosis for familial adenomatous polyposis. Surgical perception raises important advantages such as distal rectal transection under visualization, elimination of double-stapling lines (with cost-effectiveness and potential protection against suture dehiscence), elimination of dog ears, and the opportunity to be accomplished via a transanal approach after open, laparoscopic, or robotic colorectal resections. Future studies to confirm these supposed advantages are needed.
Is Fistulotomy with Marsipulization more Effective than Fistulectomy alone for Low Fistula in Ano?
Authors: Aslam Danish , Zaheer Farhan , Siddiqui Sarush Ahmed , Moosa Foad Ali , Naseer Shafaq , Arsalan Rabia , Osama Muhammed 
Published: February 2022

Abstract
Background Fistula in ano is a very common perianal condition seen in outpatient departments. Fistulotomy and fistulectomy are two conventional options of surgery. The present study is designed to observe wound healing time and mean postoperative pain score in the comparison of outcome of the fistulectomy to fistulotomy with marsupialization.
Methods This prospective randomized trial was conducted in the surgical department of the Civil Hospital Karachi for a period of 12 months, in which 60 patients with low anal fistula were divided into 2 groups. Thirty patients in group A were treated with fistulectomy, and 30 in group B were treated with fistulotomy with marsupialization. The postoperative pain severity was assessed after 24 hrs through a visual analogue scale and on weekly and fortnightly follow-ups for 6 weeks. Wound healing was assessed by clinical examination on weekly and fortnightly follow-ups for 6 weeks to estimate the mean healing time.
Results The mean pain score was significantly lower in group B in comparison to group A (3.6 ± 1.99 versus 2.40 ± 1.52; p = 0.01). The mean wound healing time was shorter in group B in comparison to group A (4.23 ± 0.77 versus 5.80 ± 0.41 weeks; p = 0.0005).
Conclusion Fistulotomy with marsupialization is a simple, easy, and more effective method than fistulectomy for the treatment of simple perianal fistula.
Fecal Calprotectin as a Predictor of COVID-19 Severity
Authors: Ahmed Mohammed Hussien , Ahmed Hebat Allah Abdel Maksoud , Nosair Nahla Abd el-Aziz , Hassan Asmaa Mbarak , Sherief Dalia ElSayed , Mahros Aya Mohammed 
Published: February 2022

AbstractCoronavirus disease 2019 (COVID-19) is highly transmittable through contact with respiratory droplets. The virus is also shed in fecal matter. Some patients may present with effects in more than one system; however, there are no defined biomarkers that can accurately predict the course or progression of the disease. The present study aimed to estimate the severity of the disease, to correlate the severity of the disease with biochemical predictors, to identify valuable biomarkers indicative of gastrointestinal disease, and to determine the cutoff values. A cross-sectional study was conducted on COVID-19 patients admitted to the Kafrelsheikh University Hospital (isolation unit) between July 10, 2020, and October 30, 2020. The diagnosis of COVID-19 was confirmed via reverse transcription-polymerase chain reaction (RT-PCR), which was employed for the detection of the viral RNA. We conclude that lymphopenia, elevated C-reactive protein (CRP) level, and liver enzymes were among the most important laboratory findings in COVID-19 patients. Statistically significant differences in platelet count, neutrophil count, D-dimer level, and fecal calprotectin levels were observed among patients presenting with chest symptoms only and patients with both chest and gastrointestinal symptoms (p = 0.004; < 0.001; 0.010; 0.003; and < 0.001, respectively). C-reactive protein, D-dimer, and fecal calprotectin levels positively correlated with disease severity. The cutoff value for fecal calprotectin that can predict gastrointestinal involvement in COVID-19 was 165.0, with a sensitivity of 88.1% and a specificity of 76.5%.
A Mobile Application to Guide Healthcare Professionals in the Correct Technique for Personal Protective Equipment Use During the COVID-19 Pandemic
Authors: Salomé Geraldo Magela , Mendonça Adriana Rodrigues dos Anjos , de Almeida Marcus Vinicius Teixeira , Miranda Flávio Dutra 
Published: February 2022

AbstractObjectives The present study aimed to develop an application to guide healthcare professionals on using personal protective equipment (PPE) during the COVID-19 pandemic and prevent and treat skin lesions caused by these devices.Methods This is a study on technological production. The framework for application development consisted in the following phases: Phase 1–“Design: identification of application development requirements;” Phase 2–“Application prototype development”: including an integrative literature review in major databases; Phase 3–“Application construction”: elaboration of the decision tree, algorithm, database structuring, and software development; Phase 4–“Transition”: application functionality test.Result Our application Simplifica EPI is an innovative technology; this software is a tool to assist healthcare professionals in using PPE. In addition, it describes how to prevent and care for skin lesions caused by PPE. Simplifica EPI has 25 screens and 32 images. It will be available on the Google Play Store after its registration with the Brazilian National Institute of Industrial Property.Conclusions After an integrative literature review, the application Simplifica EPI was developed as an innovative technology with great potential for use by healthcare professionals.
The Importance of the Therapeutic Care Plan in Colorectal Surgery
Authors: Figueiredo Henrique Carvalho e Silva , Corrêa Neto Isaac José Felippe , Mocerino Jéssica , de Oliveira Mariana Campello , Robles Laercio 
Published: February 2022

Abstract
Introduction A therapeutic plan is elaborated based on the health needs of each user, allowing a multidisciplinary team to assess diagnoses, treatment options, bonds, and optimal hospitalization time.
Objectives To identify risk management tools already used and implemented in a reference teaching hospital in the city of São Paulo and to analyze their application and risk factors in medium and large colorectal surgery.
Method Observational, longitudinal, and prospective study, with 30 patients with colorectal disease hospitalized in the surgical ward of the coloproctology service and in need of surgical treatment. In the first group, the protocol was applied with the knowledge of the researcher only, and, in the second group, with the knowledge of both the researcher and the attending physicians.
Results Sixty percent of the patients were female with a mean age of 60.93 years and body mass index (BMI) of 26.07 Kg/m2.After surgery, patients in the first group who did not receive venous thromboembolism (VTE) prophylaxis in the first 24 hours had an increased risk of having the event compared with those who returned to prophylaxis (p < 0.005), thus suggesting this prophylaxis was a protective factor against thromboembolic event (p = 0.006). This group also had a higher risk of hypoglycemia when no strict control was performed (p = 0.041).
Conclusion The compliance to hospital protocols with application monitoring, notedly in teaching places with annual admission of resident physicians, is a fundamental part of the adequate care of the patient combined with the implementation of therapeutic plans.