bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-Genome Sequencing of Bacillus spizizenii DN reveals possible mechanisms for electron transfer
Authors: Gomaa Ola M. 
Published: March 2022

Abstract
Gram positive bacteria are considered excellent candidates for bioelectrochemical systems (BES) but has not received sufficient attention as compared to the extensively studied gram negative bacteria. Bacillus spizizenii DN is a gram positive bacterium characterized as nitrate reducing, dye degrading and biofilm producing bacterium. Whole Genome sequence (WGS) of this bacterium showed Genome size 33.1 Kb and 3371 protein family. (NCBI biosample accession number: SAMN19821646). Genome annotation suggested the presence of microbial electron transfer (MET) related pathways such as nitrogen metabolism, flavonoid and anthocyanin biosynthesis, riboflavin and xenobiotic metabolism, in addition to peptidoglycan biosynthesis. Cell-wall-associated nitrate reductase as a terminal electron acceptor and surface polysaccharide suggest direct electron transfer and biofilm involvement, while the presence of cofactors, vitamins and secondary metabolites provide diffusible redox mediators for a mediated electron transfer pathway. The results indicate that Bacillus spizizenii DN showed electroactivity and can be used for different BES applications. It was further used in fabrication of nitrate biosensor, it was also used for preparing electroconductive microbeads for use in biodegradation of nitrate containing colored wastewater. WGS can be a step for acquiring knowledge about EAB to harness their electroactive capacity and to develop an efficient biodegrading strategy that accommodates the application.
Life Cycle Costing of Institutional Buildings in India: A Case Study of GRIHA-Certified CPWD Buildings
Authors: Kochhar Priyanka , Singh Mandeep 
Published: March 2022

Abstract
Buildings have an important role in mitigating, adapting, and developing resilience toward climate change. While green buildings help reduce construction’s detrimental impact on the environment, the real-estate market and select industry stakeholders remain unconvinced regarding the monetary returns of investment through building resource efficiency. Life cycle cost (LCC) assessment is an important tool used by professionals globally to assess the economic feasibility of green buildings over their service life. In India, LCC studies have been adopted for private-sector residential buildings. Moreover, municipal bodies have incentivized green buildings for private developers to offset any incremental initial cost. However, LCC calculation is yet to become mainstream within the public procurement system for institutional buildings in India. Considering that building owners and occupants would accrue any operational cost benefits of green buildings, this work aims to address issues regarding cost barriers for mainstreaming the LCC of green and sustainable institutional buildings in India. Indian public-sector organizations such as the Central Public Works Department (CPWD) have had a long-term financial interest in property, own large portfolios, and are committed to delivering a project’s best value to their clients. Accordingly, actual data from CPWD buildings and developing factors were used to create useful scenarios for future decisions based on the LCC of the buildings. Three GRIHA-rated CPWD projects were selected as case studies to investigate their LCCs. Results indicated that despite initial incremental expenditure, all the projects showed significant net savings at the end of 25 years. LCC comparison enables decision making for buildings with equal performances, while in the case of varying performances, LCC techniques relate the total LCC to identifiable units of performance to enable decisions.
­­Assessing the profile of support for potential tobacco control policies targeting availability in Great Britain: a cross-sectional population survey
Authors: Kock Loren , Shahab Lion , Moore Graham , Shortt Niamh , Pearce Jamie , Brown Jamie 
Published: March 2022

Abstract
Context: To examine the level of support for tobacco availability policies across Great Britain (GB) and associations between support for policy and sociodemographic, smoking and quitting characteristics. Methods: Cross-sectional data came from a representative survey of adults in GB (n = 2,197) during September 2021. Weighted proportions indicated the level of support for each policy. Logistic regressions estimated the associations between support for each policy and sociodemographic and smoking characteristics. Findings: There was majority support for requiring retailers to have a license which can be removed if they sell to those under-age (89.6% (95%CI 88.3–90.9)) and for restrictions on the sale of cigarettes and tobacco near schools (69.9% (67.9–71.9)). More supported than opposed raising the legal age of sale of cigarettes and tobacco to 21 (49.2% supported; 30.7% opposed; 20.1% unsure) and reducing the number of retailers selling tobacco in neighbourhoods with a high density of tobacco retailers (46.5% supported; 23.3% opposed; 30.2% unsure). More opposed than supported a ban on the sale of cigarettes and tobacco to everyone born after a certain year from 2030 onwards (41.3% opposed; 34.5% supported; 24.2% unsure). Age was positively associated with support for raising the age of sale (OR 1.06 95%CI 1.01–1.12), and inversely associated with requiring tobacco retailer licenses (OR 0.86, 0.79–0.94).Women were more likely to support raising the age of sale (OR 1.25, 1.05–1.49) and reducing the number of retailers (OR 1.34, 1.12–1.60). Current smokers were less likely to support proposed availability policies than never smokers. Smokers with high motivation to stop had higher odds of supporting a ban on tobacco sales (OR 3.45, 1.68–7.14) and reducing the density of retailers (OR 2.60, 1.29–5.27). Conclusions: Requiring tobacco retailer licensing and restrictions on sales near schools received majority support. Other tobacco availability policies received substantial support despite considerable opposition. Many of the public remain unsure. Policy Points • Strong majority support for tobacco retailer licensing and restrictions on sales near schools suggests that implementation of these tobacco availability policies would be popular among adults in Great Britain. • There was greater support than opposition to raising the legal age of sale of tobacco to 21 and reducing the number of retailers in high density tobacco retailer neighbourhoods. • Opposition to a ban on tobacco sales has weakened compared with a previous survey in England, and many of the public remain unsure; informed debate will be critical in determining public attitudes towards this tobacco availability policy.
Data-driven patient stratification and drug target discovery by using medical information and serum proteome data of idiopathic pulmonary fibrosis patients
Authors: Natsume-Kitatani Yayoi , Itoh Mari N , Takeda Yoshito , Kuroda Masataka , Hirata Haruhiko , Miyake Kohtaro , Shiroyama Takayuki , Shirai Yuya , Noda Yoshimi , Adachi Yuichi , Enomoto Takatoshi , Amiya Saori , Adachi Jun , Narumi Ryohei , Muraoka Satoshi , Tomonaga Takeshi , Kurohashi Sadao , Cheng Fei , Tanaka Ribeka , Yada Shuntaro , Aramaki Eiji , Wakamiya Shoko , Chen Yi-An , Higuchi Chihiro , Nojima Yosui , Fujiwara Takeshi , Nagao Chioko , Matsumura Yasushi , Mizuguchi Kenji , Kumanogoh Atsushi , Ueda Naonori 
Published: March 2022

Abstract
Medical information is valuable information obtained from humans regarding the phenotype of diseases. Omics data is informative to understand diseases at biomolecular level. We aimed to detect patient stratification patterns in a data-driven manner and identify candidate drug targets by investigating biomolecules that are linked to phenotype-level characteristics of a targeted disease. Such data integration is challenging because the data types of them are different, and these data contain many items that are not directly related to the disease. Hence, we developed an algorithm, subset binding, to find inter-related attributes in heterogeneous data. To search for potential drug targets for intractable IPF (idiopathic pulmonary fibrosis), we collected medical information and proteome data of serum extracellular vesicles from patients with interstitial pneumonia including IPF. Our approach detected 20 proteins linked with IPF characteristics, whose expression intensities were confirmed to be high in fibrotic areas of human lung tissues. Furthermore, ponatinib, which inhibits these proteins, suppressed EMT (epithelial mesenchymal transition) in vitro. This workflow paves the way for data-driven drug target discovery even for intractable diseases whose mechanisms of pathogenesis are not fully understood.
Analisis Kesesuaian Kompetensi Dasar dengan Bahan Ajar Bahasa Arab MTs Kelas IX Karya Yushi M. Mahmudah
Authors: Mufti Ali , Mudrofin Mudrofin 
Published: March 2022

The Indonesian Ministry of Religion has published Arabic textbooks for ninth grade to assist the implementation of the revision of the Core and Basic Competencies. The purpose of this research is to evaluate the compatibility of Arabic teaching materials with the Basic Competencies outlined in KMA No. 183 of 2019. The study employs a library research design with the data collected using documentation. The sample was selected using purposive sampling techniques, namely the content examination and assessment. Content analysis was performed to analyze the data. The results showed that some contents of the textbook did not comply with the requirements of the basic competence. Several readings and conversational texts did not instance of grammatical structures in use. The textbook did not contain practice questions for mahrah istima'. Also, only 13 (out of 34) questions in the mahārah istimā' segment pertain to higher-order thinking skills (HOTS), while the majority of the questions in mahārah kalām and kitābah pertains to the basic competence of 2013 Curriculum.
The Effects of Curcumin Supplementation on Metabolic Biomarkers and Body Mass
Index in Patients with Nonalcoholic Fatty Liver Disease: A Systematic Review and
Meta-analysis of Randomized Controlled Trials
Authors: Khalili Leila , Nammi Srinivas 
Published: March 2022

Background and Aims:
Curcumin is one of the most commonly used indigenous molecules endowed
with various shielding functionalities that protect the liver. In the present research, we aimed to investigate
the effects of curcumin on metabolic factors and body mass index (BMI) in patients with non-alcoholic fatty
liver disease (NAFLD) using a meta-analysis of randomized, controlled trials.

Methods::
Online databases PubMed, Embase, Web of Science, and Science Direct were searched until April
2021 to identify eligible articles. Fourteen trials were included.

Results:
The results showed that curcumin consumption can significantly reduce AST (-0.35, (-0.57 to
-0.14)), total cholesterol (-0.81, (-1.34 to -0.27)), TG (-0.49, (-0.71 to -0.27)), and FBS (-0.28, (-0.46 to -0.09))
in patients with NAFLD. However, the improvements in ALT (-0.29, (-0.58 to 0.00)), LDL (-0.48, (-0.97 to
0.01)), HDL (0.03, (-0.38 to 0.44)), and BMI (-0.13, (-0.29 to 0.02)) were not statistically significant. Furthermore,
the findings revealed that the optimal dose and duration of curcumin consumption for patients with
NAFLD is <500 mg/d for less than 10 weeks.

Conclusions:
The present study suggests that consuming curcumin can improve liver enzymes, lipid profile,
FBS, and BMI in patients with NAFLD. Moreover, curcumin supplementation may provide beneficial effects
on metabolic biomarkers and body weight if used at the appropriate dose and duration. Further RCTs are
required to confirm our findings.
Indole-3-carbinol (I3C) as Leukemia Therapeutic Agents: Review
Authors: Hassanshahi Gholamhossein , Karimabad Mojgan Noroozi , Mohamadi Maryam , Torabizadeh Seyedeh Atekeh 
Published: March 2022

Abstract:
Leukemia or blood cancer was initially discovered in 1845 and this malignancy was reported in patients who had an amplified number of blood cells, in particular, white blood cells (WBC), due to this disease. The event of leukemia was further identified as a malignant hematopoietic disorder due to both uncontrolled and unlimited proliferation in combination with lack of differentiation of the leukemic stem cells.Furethermore, 75 to 80% of the global population use herbal remedies as primary therapy, mainly because of their better efficiency and satisfaction, which elevate the human body symmetry with the minimum unwanted adverse effects. For the control of cancer, plant products, and fruits have been considered promising tools and are being consumed for centuries. Several plant extracts are also being used for the therapy and prevention of different types of known cancers. Indole-3-carbinol (I3C) is a natural material obtained from Brassica diversity of vegetables and has been reported to serve as a promising cancer preventative agent. In the present review, theauthors mainly tried to focus and emphasize I3C applications in the leukemia treatment.
Second-line Therapy for Helicobacter Pylori Eradication: State of the Art
Authors: Losurdo Giuseppe , D’Abramo Fulvio Salvatore , Piazzolla Mariapaola , Rima Raffaella , Continisio Antonio , Pricci Maria , Ierardi Enzo , Di Leo Alfredo 
Published: March 2022

Abstract:
Helicobacter pylori (H. pylori) is a Gram negative spiraliform bacterium that colonizes the human stomach. It is the most common cause of chronic gastritis, peptic ulcer, and gastric carcinoma. The eradication therapy is based on the combination of a proton pump inhibitor and several antibiotics such as amoxicillin, metronidazole, clarithromycin, levofloxacin or tetracycline. The most commonly used regimens for eradication in the first line are triple, sequential and concomitant therapy, despite the last European Guidelines suggesting a quadruple therapy already at the first attempt in areas with high resistance rates. However, the rise in antibiotic resistance is the main reason for a marked increase in first-line therapy failure. Clarithromycin resistancea, is especially acknowledged as the most important event resulting in failure. Up to 20% of patients are intended not to eradicate, therefore they will need a second line therapy. Currently, the most used rescue regimens are levofloxacin-based triple therapy and bismuth-containing quadruple therapy, despite guidelines suggesting to use a combination of antibiotics that have not been included in previous treatments. Nitazoxanide is a novel antibiotic with promising results. Additionally, an interesting field worth of investigation is the antibiotic susceptibility based approach, which could help choose antibiotics with confirmed effectiveness in vitro. Analysis of antibiotic resistance may be performed by both bacterial culture and molecular biology techniques, able to detect point mutations conferring resistance. This is a particularly interesting approach, since it may personalize the therapy, thus optimizing the regimen and maximizing the probability of success.
Complex Processes Underlying the Dynamic Changes of D-serine Levels in
AD Brains
Authors: Ni Xiance , Mori Hisashi 
Published: March 2022

Background:
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by
extracellular β-amyloid (Aβ) plaques and cognitive impairments. D-Serine, produced by the enzyme
serine racemase (SR) in the brain, functions as an endogenous co-agonist at the glycine-binding site
of N-methyl-D-aspartate receptor (NMDAR), has been implicated in the pathophysiological progression
of AD.

Purpose:
Evidence regarding the understanding of the role and dynamic modulation of D-serine during
AD progression remains controversial. This literature review aims to offer novel research directions
for studying the functions and metabolisms of D-serine in AD brains.

Method:
We searched PubMed, using D-serine/SR and AD as keywords. Studies related to NMDAR
dysfunction, neuronal excitotoxicity, D-serine dynamic changes and inflammatory response were included.

Results:
This review primarily discusses: (i) Aβ oligomers’ role in NMDAR dysregulation, and the
subsequent synaptic dysfunction and neuronal damage in AD, (ii) D-serine’s role in NMDAR-elicited
excitotoxicity, and (iii) the involvement of D-serine and SR in AD-related inflammatory pathological
progression.

Conclusion:
We also presented supposed metabolism and dynamic changes of D-serine during AD
progression and hypothesized that: (i) the possible modulation of D-serine levels or SR expression as
an effective method of alleviating neurotoxicity during AD pathophysiological progression, and (ii)
the dynamic changes of D-serine levels in AD brains possibly resulting from complex processes.
Novel Derivatives Based on Zerumbone Scaffold as Potential Anticancer
Inhibitors
Authors: Hieu Luu Xuan , Ha Tran Thi Hong , Chi Hoang Kim , Vu Tran Khac , Chung Pham Van , Hung Truong Ngoc , Chinh Luu Van 
Published: March 2022

Abstract:
A two-step procedure was applied to couple zerumbone, a natural sesquiterpene, with thiols
8a-k to obtain a small library of ten novel zerumbone derivatives 9a-k with full-length library data of
spectra including 1H-, 13C-NMR, and HRMS. The tautomerization of 9a, 9b, and 9c was revealed in
DMSO and discussed in the case of 9c. The series of 9a-k together with zerumbone 1 was evaluated
for their in vitro cytotoxic activity using three human cancer cell lines, HepG2, A549 and HeLa. The
results revealed that all zerumbone derivatives had cytotoxic activity against HepG2, A549, and HeLa
cells that was 4-20 times stronger than zerumbone.
Multiple Leiomyomas in a Patient with Benign Metastasizing Leiomyoma:
A Case Report
Authors: Liu Shuangjiao , Zhou Wenming , Fu Weidong 
Published: March 2022

Introduction:
Benign metastasizing leiomyoma (BML) is a rare disease and mostly affects
females with a history of uterine leiomyoma, and particularly the presence of multiple leiomyomas in
BML patients is extremely rare.

Case Presentation:
This paper reported the clinical and imaging data of a BML patient with multiple
leiomyomas involving bilateral pulmonary, mediastinum, pericardium, spine, peritoneum, and left thigh.
Multiple BML lesions exhibited consistent imaging examinations, significantly improving the delayed
phase enhancement. After multi-stage targeted therapy for multiple systemic metastases and the development
of drug resistance, the patient was treated with hysterectomy and bilateral adnexectomy along
with letrozole-based endocrine therapy. BML lesions, both pulmonary and mediastinum, became significantly
smaller than before.

Conclusion:
This paper aims to analyze the imaging and clinical features of multiple leiomyomas in this
BML case, thus strengthening the understanding of the rare type of leiomyoma for effective preoperative
diagnosis and clinical treatment. Furthermore, it is noteworthy that gynecologists should avoid the
manifestation of BML when performing uterine fibroids surgery.
α-Glucosidase and α-Amylase Inhibition Study andIn SilicoAnalysis ofMimosa pudicaL. of Nepalese Origin
Authors: Shrestha Dipesh , Pokhrel Tamlal , Dhakal Kamal , Pandey Anisha , Sharma Prabha , Sapkota Sima , Adhikari Achyut 
Published: March 2022

Background:Diabetes has become a considerably more frequent condition and has increased alarmingly in recent years, possibly due to the adoption of modern lifestyle and food habits. The two prominent features of diabetes mellitus are high blood glucose and insulin deficiency, leading to severe consequences. Developing next-generation anti-diabetic medicines with fewer side effects has been a major focus in this situation.Objective:This research aimed to investigate the total phenolic and flavonoid content, antioxidant, antibacterial, α-amylase, and α-glucosidase inhibition activity, as well as in silico analysis of Mimosa pudica L.Methods:The inhibitory activity against α-amylase and α-glucosidase was performed using CNPG3 and PNPG, respectively. Antioxidant activity was estimated using DPPH free radical scavenging assay. The well diffusion method was used for the antibacterial. Using folin- ciocalteu’s reagent, the total phenolic content was determined. The total flavonoid content was determined using the aluminium trichloride method. In addition, molecular docking was performed using autodock vina.Results:Inhibition of α-glucosidase (IC50 = 1.059±0.14μg/mL) was found to be more significant than α-amylase (IC50 = 164.9±0.95μg/mL). The plant was also found to have antioxidant activity (IC50 = 8.207 ±0.23μg/mL), as well as antibacterial activity against Staphylococcus aureus (ZOI = 13mm) and Bacillus subtilis (ZOI = 10mm). Similarly, the total phenolic and flavonoid content was found to be 177.93±1.8 mg GAE/g, and 19.747±6.11 mg QE/g, respectively. In addition, compounds (stigmasterol, quercetin, and avicularin) isolated from M. pudica showed perfect binding to the enzyme’s active site.Conclusion:Mimosa pudica of Nepalese origin possess potent inhibition against digestive enzymes. Therefore, M. pudica can be used as an alternative therapeutic source to combat the global threat of diabetes.
The Toxic Effects of Endocrine Disrupting Chemicals (EDCs) on Gut
Microbiota: Bisphenol A (BPA) A Review
Authors: Charitos Ioannis Alexandros , Topi Skender , Gagliano-Candela Roberto , De Nitto Emanuele , Polimeno Lorenzo , Montagnani Monica , Santacroce Luigi 
Published: March 2022

Background:
Bisphenol A (BPA), an important industrial material widely applied in daily products, is considered an endocrine-disrupting chemical that may adversely affect humans. Grow-ing evidence has shown that intestinal bacterial alterations caused by BPA exposure play an im-portant role in several local and systemic diseases.

Aim:
finding evidence that BPA-induced alterations in gut microbiota composition and activity may perturb its role on human health.

Results:
Evidence from several experimental settings shows that both low and high doses of BPA in-terfere with the hormonal, homeostatic, and reproductive systems in animals and humans. Moreover, it has recently been classified as an environmental obesogenic, with metabolic-disrupting effects on lipid metabolism and pancreatic b-cell functions. Several evidence characterizes PBA as an envi-ronmental contributor to type II diabetes, metabolic syndromes, and obesity. However, the highest estimates of the exposure derived from foods alone or in combination with other sources are 3 to 5 times below the new tolerable daily intake (TDI) value, today reduced by the European Food Safety Authority (EFSA) experts from 50 micrograms per kilogramme of bodyweight per day (μg/kg bw/day) to 4 μg/kg bw/day.

Conclusions:
Considering estimates for the total amount of BPA that can be ingested daily over a lifetime, many International Health Authorities conclude that dietary exposure of adult humans to BPA does not represent a risk to consumers' health, declaring its safety due to very-low established levels in food and water and any appreciable health risk.