bluuebunny/crossref_metadata_2025_split huggingface dataset:
E-Marketing Sebagai Bentuk Pemasaran Darurat Dalam Meningkatkan Minat Pendaftar Pada Masa Pandemi Covid-19
Authors: Dekanawati Vivid , Fatimah Siti , Purnomo Cahya , Sahudiyono Sahudiyono , Supartini Supartini , Atinahsyani Aisyah 
Published: March 2022

Penelitian ini bertujuan untuk menemukan cara efektif bagaimana menarik perhatian pendaftar sebagai taruna baru di Sekolah Tinggi Maritim Yogyakarta dimasa pandemi Covid-19. Subyek penelitian ini adalah Taruna baru semester satu jurusan Diploma 3 Manajemen Transportasi Laut, Diploma 3 Permesinan Kapal, Diploma 3 Studi Nautika dan S1 Transportasi dikarenakan taruna tersebut masih sangat baru masuk menjadi taruna di Sekolah Tinggi Maritim Yogyakarta. Obyek Penelitian ini adalah E-Marketing, Pemasaran Darurat, Pemasaran di era Pandemi Covid-19 di Sekolah Tinggi Maritim Yogyakarta. Dari sisi perguruan tinggi sekolah maritim yang ada di Yogyakarta ini menunjukkan keunikannya dimana Yogyakarta belum mempunyai pelabuhan komersial untuk kapal-kapal cargo namun mampu mencetak lulusan di bidang kepelabuhanan dan kemaritiman. Metode pengumpulan data dalam penelitian ini menggunakan kuesioner. Analisis data menggunakan metode analisis regresi linier berganda. Hasil penelitian ini adalah variabel-variabel E-Marketing dan Pemasaran Darurat berpengaruh secara simultan terhadap pemasaran yang dilakukan di era Pandemi Covid-19, dimana interaksi tatap muka dibatasi. Pengaruh variabel independen yaitu e-marketing dan pemasaran darurat terhadap variabel dependen pemasaran yang dilakukan di era Pandemi Covid-19 adalah 70%. Sedangkan 30% dipengaruhi oleh variabel lain selain E-Marketing dan Pemasaran Darurat. Ini artinya eletronik marketing sangat efektif untuk menarik minat pendaftar.
Investigation of Lysozyme Diffusion in Agarose Hydrogels Employing a Microfluidics-Based UV Imaging Approach
Authors: Wenger Lukas , Hubbuch Jürgen 
Published: March 2022

Hydrogels are polymer-based materials with a high water content. Due to their biocompatible and cell-friendly nature, they play a major role in a variety of biotechnological applications. For many of these applications, diffusibility is an essential property influencing the choice of material. We present an approach to estimate diffusion coefficients in hydrogels based on absorbance measurements of a UV area imaging system. A microfluidic chip with a y-junction was employed to generate a fluid-hydrogel interface and the diffusion of lysozyme from the fluid into the hydrogel phase was monitored. Employing automated image and data processing, analyte concentration profiles were generated from the absorbance measurements and fits with an analytical solution of Fick’s second law of diffusion were applied to estimate diffusion coefficients. As a case study, the diffusion of lysozyme in hydrogels made from different concentrations (0.5–1.5% (w/w)) of an unmodified and a low-melt agarose was investigated. The estimated diffusion coefficients for lysozyme were between 0.80 ± 0.04×10−10 m2 s−1 for 1.5% (w/w) low-melt agarose and 1.14 ± 0.02×10−10 m2 s−1 for 0.5% (w/w) unmodified agarose. The method proved sensitive enough to resolve significant differences between the diffusion coefficients in different concentrations and types of agarose. The microfluidic approach offers low consumption of analyte and hydrogel and requires only relatively simple instrumentation.
Optimize the Preparation of Novel Pyrite Tailings Based Non-sintered Ceramsite by Plackett-Burman Design Combined With Response Surface Method for Phosphorus Removal
Authors: Chen Ruihuan , Pan Zhenlin , Chu Shuyi , Xiao Jibo , Weng Rengui , Ouyang Da , Yang Yunlong , Wu Xiangting , Huang Zhida 
Published: March 2022

The large amount of untreated pyrite tailings has caused serious environmental problems, and the recycling of pyrite tailings is considered as an attractive strategy. Here, we reported a novel non-sintered ceramsite prepared with pyrite tailings (PTNC) as the main active raw material for phosphorus control, and the dosage effect of ingredients on total phosphorus (TP) removal ability was investigated. The results from Plackett-Burman Design (PBD) suggested the dosages of dehydrated sludge, sodium bicarbonate, and cement were the factors which significantly affect the TP removal ability. The Box-Behnken Design (BBD) based response surface methodology was further employed, and it indicated the interactions between different factors, and the optimized recipe for PTNC was 84.5 g (pyrite tailings), 10 g (cement), 1 g (calcined lime), 1 g (anhydrous gypsum), 3 g (dehydrated sludge), and 0.5 g (sodium bicarbonate). The optimized PTNC was characterized and which presented much higher specific area (7.21 m2/g) than the standard limitation (0.5 m2/g), as well as a lower wear rate (2.08%) rather than 6%. Additionally, the leaching metal concentrations of PTNC were far below the limitation of Chinese National Standard. The adsorption behavior of TP on PTNC was subsequently investigated with batch and dynamic experiments. It was found that the calculated max adsorption amount (qmax) was about 7 mg/g, and PTNC was able to offer a stable TP removal ability under different hydraulic retention time (HRT). The adsorption mechanism was discussed by model fitting analysis combined with XRD and SEM characterization, and cobalt phosphide sulfide was observed as the newly formed substance through the adsorption process, which suggested the existing of both physical and chemical adsorption effect. Our research not only offered an economic preparation method of ceramsite, but also broadened the recycling pathway of pyrite tailings.
Sepsis Prediction for the General Ward Setting
Authors: Yu Sean C. , Gupta Aditi , Betthauser Kevin D. , Lyons Patrick G. , Lai Albert M. , Kollef Marin H. , Payne Philip R. O. , Michelson Andrew P. 
Published: March 2022

ObjectiveTo develop and evaluate a sepsis prediction model for the general ward setting and extend the evaluation through a novel pseudo-prospective trial design.DesignRetrospective analysis of data extracted from electronic health records (EHR).SettingSingle, tertiary-care academic medical center in St. Louis, MO, USA.PatientsAdult, non-surgical inpatients admitted between January 1, 2012 and June 1, 2019.InterventionsNone.Measurements and Main ResultsOf the 70,034 included patient encounters, 3.1% were septic based on the Sepsis-3 criteria. Features were generated from the EHR data and were used to develop a machine learning model to predict sepsis 6-h ahead of onset. The best performing model had an Area Under the Receiver Operating Characteristic curve (AUROC or c-statistic) of 0.862 ± 0.011 and Area Under the Precision-Recall Curve (AUPRC) of 0.294 ± 0.021 compared to that of Logistic Regression (0.857 ± 0.008 and 0.256 ± 0.024) and NEWS 2 (0.699 ± 0.012 and 0.092 ± 0.009). In the pseudo-prospective trial, 388 (69.7%) septic patients were alerted on with a specificity of 81.4%. Within 24 h of crossing the alert threshold, 20.9% had a sepsis-related event occur.ConclusionsA machine learning model capable of predicting sepsis in the general ward setting was developed using the EHR data. The pseudo-prospective trial provided a more realistic estimation of implemented performance and demonstrated a 29.1% Positive Predictive Value (PPV) for sepsis-related intervention or outcome within 48 h.
Randomized Clinical Trial: Probiotics Alleviated Oral-Gut Microbiota Dysbiosis and Thyroid Hormone Withdrawal-Related Complications in Thyroid Cancer Patients Before Radioiodine Therapy Following Thyroidectomy
Authors: Lin Baiqiang , Zhao Fuya , Liu Yang , Wu Xin , Feng Jing , Jin Xiangren , Yan Wei , Guo Xiao , Shi Shang , Li Zhiyong , Liu Lujia , Chen Hongye , Wang Haoran , Wang Shuang , Lu Yu , Wei Yunwei 
Published: March 2022

BackgroundThyroid hormone withdrawal (THW) in postoperative thyroid cancer patients who need always accompanied by complications (e.g., dyslipidemia and constipation). At present, there are no effective and safe means to alleviate these complications.PurposeWe aimed to assess the oral-gut microbiota profiles in THW patients then investigate whether probiotics could alleviating alleviate THW related complications and investigate whether these therapeutic effects were associated with the oral-gut microbiota state.MethodsFifty eligible thyroid carcinoma patients undergoing thyroidectomy were randomly assigned to receive probiotics or placebo during THW. Complications were assessed through validated questionnaires and plasma lipid indicators. The complex probiotics preparation was composed of Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis, and Bacillus cereus.ResultsProbiotics alleviated lack of energy, constipation, weight gain, and dry mouth and decreased the levels of fecal/serum LPS and plasma lipid indicators (total cholesterol, triglycerides, low-density lipoprotein, and apolipoprotein A) (P &lt; 0.05). Gut and oral microbial diversity were significantly decreased after THW, while an increased microbial dysbiosis index (MDI) was observed. Probiotics distinctly restored the gut and oral microbial diversity. Increased Holdemanella, Enterococcus, and Coprococcus_2, while decreased Fusobacterium, Eubacterium_ruminantium_group, Ruminococcus_1, and Parasutterella in the gut were found after probiotics intervention. Lack of energy, constipation, weight gain, and dyslipidemia were seen to be related to the above microbiota. In addition, probiotics reduced oral Prevotella_9, Haemophilus, Fusobacterium, and Lautropia, which were positively correlated with the occurrence of dry mouth.ConclusionProbiotics reduce the incidence of complications in patients after THW, which may be related to modifying the oral and gut microbiota.Clinical Trial Registration[https://clinicaltrials.gov/], identifier America Clinical Trial Registry NCT03574051.
Residue Monitoring of Propiconazole in the Rice–Crab Co-Culture Field and its Toxicity and Bioaccumulation to Eriocheir sinensis
Authors: Yu Lina , Li Changsheng , Zhang Yuting , Guo Xuanjun , Cao Niannian , Guo Shuxin , Wu Sijia , Li Xuefeng , Pang Sen 
Published: March 2022

Rice–crab co-culture is a high-benefit eco-breeding pattern that has been extensively developed in many regions of China. However, little attention has been paid to the safety of pesticides used to control rice pests in the crab–rice co-culture system. This study monitored the actual residue levels of propiconazole in water and soil of rice–crab co-culture fields and evaluated the acute toxicity, subchronic toxicity, and bioaccumulation of propiconazole to Eriocheir sinensis. We observed that the residue level of propiconazole in paddy soil was higher than that in paddy water within 42-day field monitoring. Propiconazole demonstrated a low acute toxicity (96 h-LC50 &gt; 100 mg/L) to E. sinensis and exhibited no obvious adverse impact on the growth of E. sinensis after exposure to 500 μg/L, which was 10 times the actual residual concentration of propiconazole in the crab–rice co-culture field. The highest bioaccumulation of propiconazole was obtained from gills, followed by the hepatopancreas and meat. These results will contribute to the guidance of scientific utilization of pesticides in the crab–rice co-culture field.
GPC2 Is a Potential Diagnostic, Immunological, and Prognostic Biomarker in Pan-Cancer
Authors: Chen Guoming , Luo Dongqiang , Zhong Nan , Li Danyun , Zheng Jiyuan , Liao Hui , Li Zhuoyao , Lin Xiaoxiao , Chen Qiqi , Zhang Cheng , Lu Yuanjun , Chan Yau-Tuen , Ren Qing , Wang Ning , Feng Yibin 
Published: March 2022

BackgroundGlypican 2 (GPC2), a member of glypican (GPC) family genes, produces proteoglycan with a glycosylphosphatidylinositol anchor. It has shown its ascending significance in multiple cancers such as neuroblastoma, malignant brain tumor, and small-cell lung cancer. However, no systematic pan-cancer analysis has been conducted to explore its function in diagnosis, prognosis, and immunological prediction.MethodsBy comprehensive use of datasets from The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), Genotype-Tissue Expression Project (GTEx), cBioPortal, Human Protein Atlas (HPA), UALCAN, StarBase, and Comparative Toxicogenomics Database (CTD), we adopted bioinformatics methods to excavate the potential carcinogenesis of GPC2, including dissecting the correlation between GPC2 and prognosis, gene mutation, immune cell infiltration, and DNA methylation of different tumors, and constructed the competing endogenous RNA (ceRNA) networks of GPC2 as well as explored the interaction of GPC2 with chemicals and genes.ResultsThe results indicated that GPC2 was highly expressed in most cancers, except in pancreatic adenocarcinoma, which presented at a quite low level. Furthermore, GPC2 showed the early diagnostic value in 16 kinds of tumors and was positively or negatively associated with the prognosis of different tumors. It also verified that GPC2 was a gene associated with most immune-infiltrating cells in pan-cancer, especially in thymoma. Moreover, the correlation with GPC2 expression varied depending on the type of immune-related genes. Additionally, GPC2 gene expression has a correlation with DNA methylation in 20 types of cancers.ConclusionThrough pan-cancer analysis, we discovered and verified that GPC2 might be useful in cancer detection for the first time. The expression level of GPC2 in a variety of tumors is significantly different from that of normal tissues. In addition, the performance of GPC2 in tumorigenesis and tumor immunity also confirms our conjecture. At the same time, it has high specificity and sensitivity in the detection of cancers. Therefore, GPC2 can be used as an auxiliary indicator for early tumor diagnosis and a prognostic marker for many types of tumors.
Orbital Apex Syndrome: A Case Series in a Tertiary Medical Center in Southern Taiwan
Authors: Lee Peng-Hsuan , Shao Shih-Chieh , Lee Wan-Ju Annabelle 
Published: March 2022

BackgroundOrbital apex syndrome (OAS) is a rare ocular complication following by infection, inflammation, trauma, neoplasms, and vascularity. The epidemiological features of OAS remained limited, so this study aimed to present ophthalmic clinical features, determine the causes to evaluate the visual prognosis of orbital apex syndrome (OAS) patients in Taiwan.MethodsThis was a retrospective study by reviewing the electronic medical records from National Cheng Kung University Hospital in Taiwan during 2017–2019. We included patients diagnosed with OAS to review their ocular symptoms and signs, visual acuity, ocular images, etiologies, treatment and visual prognosis.ResultsTwenty cases (mean age: 65.55 ± 13.06; male: 75%) with the diagnosis of OAS were included in this study. All patients presented as unilateral involvement, but the initial ocular presentations and etiologies varied. For example, blurred vision was reported in 80% of these patients, and tumor-related compression (55%) and infection (15%) were the most frequent causes for the OAS. After the follow-up, we found 35% of patients' visions declined or worsened to the blindness, 15% of patients' visions remained stable, 20% of patients' visions had mild improvement, and 35% of patients' visions were not measured because of debilitating clinical condition. We identified three OAS patients with mortality (15%), and all of them were attributed to the underlying malignancies.ConclusionThe clinical magnifications and etiologies of OAS are heterogeneous in Taiwan. Our findings indicated the tumor-related compression is the most frequent causes of OAS in Taiwan, and it is also related to poor clinical outcomes.
Different Complement Activation Pathways Underly Cognitive Impairment and Type 2 Diabetes Mellitus Combined With Cognitive Impairment
Authors: Li Zhenxing , Zhang Weiwei , Gao Feng , Tang Qiqiang , Kang Dongmei , Shen Yong 
Published: March 2022

BackgroundThe immune response and the complement system are associated with cognitive impairment and diabetes mellitus, respectively. Activation of the complement system in these diseases occurs mainly through either the classical pathway or the alternative pathway. However, the specific complement proteins involved in the development of the type 2 diabetes mellitus (T2DM) and cognitive impairment are still unclear. Here, we investigated complement proteins in serum from patients with T2DM, cognitive impairment, or both T2DM and cognitive impairment.ObjectiveTo investigate the levels of serum immune complement proteins in patients with T2DM, cognitive impairment, or T2DM combined with cognitive impairment and the associations between these complement proteins and risk factors for T2DM or cognitive impairment.MethodsClinical markers were collected from blood samples of 264 participants. Luminex multiplex assays were used to detect serum complement proteins. All statistical analyses were performed using Prism or R studio.ResultsThere was a difference in serum levels of the complement proteins C1q, C3, C3b, and FH between the three different groups. Hyperglycemia was significantly correlated with elevated C3b or reduced C3, C1q, and FH. In addition, hyperlipidemia was positively correlated with elevated levels of C3, C4, C1q, and FH proteins. There was an association between C1q, C3, C4, and FH and β-pancreas cell function, whereas only FH was associated with insulin resistance. Higher serum C1q was significantly associated with an increased risk of cognitive impairment.ConclusionSerum levels of complement proteins were closely associated with hyperglycemia and hyperlipidemia. We found that classical complement pathway activation mainly occurred in the cognitive impairment only group, whereas the alternative pathway may reflect T2DM and T2DM with cognitive impairment.
Eucommia ulmoides Oliver-Tribulus terrestris L. Drug Pair Regulates Ferroptosis by Mediating the Neurovascular-Related Ligand-Receptor Interaction Pathway- A Potential Drug Pair for Treatment Hypertension and Prevention Ischemic Stroke
Authors: Zhang Qian , Yang Jie , Yang Chuanhua , Yang Xuesong , Chen Yongzhi 
Published: March 2022

BackgroundIn this study, we used the network pharmacology approach to explore the potential disease targets of the Eucommia ulmoides Oliver (EUO)-Tribulus terrestris L. (TT) drug pair in the treatment of hypertension-associated neurovascular lesions and IS via the ferroptosis pathway.MethodsWe used the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform to search for the key active compounds and targets of the drug pair. Based on the GeneCards database, the relevant targets for the drug pair were obtained. Then, we performed the molecular docking of the screened core active ingredients and proteins using the DAVID database and the R AutoDock Vina software. Based on the GSE22255 dataset, these screened target proteins were used to build random forest (RF) and support vector machine (SVM) models. Finally, a new IS nomogram prediction model was constructed and evaluated.ResultsThere were 36 active compounds in the EUO-TT drug pair. CHRM1, NR3C1, ADRB2, and OPRD1 proteins of the neuroactive ligand-receptor interaction pathway interacted with the proteins related to the ferroptosis pathway. Molecular docking experiments identified 12 active ingredients of the drug pair that may tightly bind to those target proteins. We constructed a visual IS nomogram prediction model using four genes (CHRM1, NR3C1, ADRB2, and OPRD1). The calibration curve, DCA, and clinical impact curves all indicated that the nomogram model is clinically applicable and diagnostically capable. CHRM1, NR3C1, ADRB2, and OPRD1, the target genes of the four effective components of the EUO-TT drug pair, were considered as risk markers for IS.ConclusionsThe active ingredients of EUO-TT drug pair may act on proteins associated with the neuroactive ligand-receptor interaction pathway to regulate ferroptosis in vascular neurons cells, ultimately affecting the onset and progression of hypertension.
“GENYAL” Study to Childhood Obesity Prevention: Methodology and Preliminary Results
Authors: Marcos-Pasero Helena , Aguilar-Aguilar Elena , de la Iglesia Rocío , Espinosa-Salinas Isabel , Molina Susana , Colmenarejo Gonzalo , Martínez J. Alfredo , Ramírez de Molina Ana , Reglero Guillermo , Loria-Kohen Viviana 
Published: March 2022

ObjectiveThis article describes the methodology and summarizes some preliminary results of the GENYAL study aiming to design and validate a predictive model, considering both environmental and genetic factors, that identifies children who would benefit most from actions aimed at reducing the risk of obesity and its complications.DesignThe study is a cluster randomized clinical trial with 5-year follow-up. The initial evaluation was carried out in 2017. The schools were randomly split into intervention (nutritional education) and control schools. Anthropometric measurements, social and health as well as dietary and physical activity data of schoolchildren and their families are annually collected. A total of 26 single nucleotide polymorphisms (SNPs) were assessed. Machine Learning models are being designed to predict obesity phenotypes after the 5-year follow-up.SettingsSix schools in Madrid.ParticipantsA total of 221 schoolchildren (6–8 years old).ResultsCollected results show that the prevalence of excess weight was 19.0, 25.4, and 32.2% (according to World Health Organization, International Obesity Task Force and Orbegozo Foundation criteria, respectively). Associations between the nutritional state of children with mother BMI [β = 0.21 (0.13–0.3),p(adjusted) &lt;0.001], geographical location of the school [OR = 2.74 (1.24–6.22),p(adjusted) = 0.06], dairy servings per day [OR = 0.48 (0.29–0.75),p(adjusted) = 0.05] and 8 SNPs [rs1260326, rs780094, rs10913469, rs328, rs7647305, rs3101336, rs2568958, rs925946;p(not adjusted) &lt;0.05] were found.ConclusionsThese baseline data support the evidence that environmental and genetic factors play a role in the development of childhood obesity. After 5-year follow-up, the GENYAL study pretends to validate the predictive model as a new strategy to fight against obesity.Clinical Trial RegistrationThis study has been registered inClinicalTrials.govwith the identifier NCT03419520,https://clinicaltrials.gov/ct2/show/NCT03419520.