bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome engineering of the Corynebacterium glutamicum chromosome by the Dual-In/Out strategy
Authors: Lobanova Juliya S. , Gorshkova Natalya V. , Krylov Alexander A. , Stoynova Nataliya V. , Mashko Sergey V. 
Published: March 2022

Abstract
The combination of recipient chromosome modification due to recombineering by a linear dsDNA fragment with two types of site-specific recombination (SSR) systems, which were previously developed for Escherichia coli and called Dual-In/Out, followed by transfer of the provided genome modifications by homologous recombination between the target strain genome and isolated donor chromosomal DNA penetrating the cell due to electroporation results in a novel gene engineered editing strategy for Corynebacterium glutamicum. This strategy was based on (i) E. coli Rac prophage RecE564/RecT-dependent recombineering; (ii) corynephage φ16 (Int/Xis)- and E. coli phage P1 Cre-mediated site-specific recombination systems; and (iii) the development of a C. glutamicum electrotransformation protocol with donor chromosomal DNA. Thus, two antibiotic resistance-marked chromosomal modifications were selectively combined in a targeted C. glutamicum strain that already contained another modification. For each tested C. glutamicum strain, the efficiency of the different desired modifications for electrotransformation could fluctuate significantly (up to two orders of magnitude), likely due to the recombinogenic accessibility of the corresponding locus of the bacterial chromosome. It was found that the expression of RecE564/RecT coding genes in the recipient strain facilitated the inheritance of the penetrated DNA. It was supposed that the developed strategy in general and its separate elements could be helpful for enhancing the genetic toolbox needed for genome editing of targeted C. glutamicum strains.
An increase in psychosomatic symptoms among youth: Is it connected to adult mental health?
Authors: Giannotta Fabrizia , Nilsson Kent W. , Åslund Cecilia , Larm Peter 
Published: March 2022

Abstract
Background. Despite an increase in mental health problems, with psychosomatic symptoms having been observed in new generations of youth, the extent to which these problems correspond to an increase in adult mental problems is unknown. The present study investigates whether adolescents with high levels of psychosomatic symptoms are at risk of developing depression and anxiety problems in adulthood and whether sex moderates any association. Moreover, we aim to understand whether different clusters of youth psychosomatic symptoms – somatic, psychological and musculoskeletal – have different impacts on adult mental health. Methods. 1545 Swedish adolescents – aged 13 (49%) and 15 (51%) – completed surveys at baseline (T1) and 3 years later (T2); of them, 1174 (61% females) also participated after 6 years (T3). Multivariate logistic models were run. Results. Youth with high levels of psychosomatic symptoms had higher odds of high levels of depressive symptoms at T2 and T3. Moreover, psychosomatic symptoms at T1 predicted a high level of anxiety symptoms and diagnoses of anxiety disorders at T3. When analyzed separately, musculoskeletal symptoms predicted higher odds of having high levels of depressive symptoms at T2 and T3 and anxiety symptoms at T3, while psychological symptoms predicted high levels of anxiety symptoms at T2. Moreover, somatic symptoms at T1 predicted diagnoses of depression and anxiety disorders at T3. Sex did not moderate any of the relationships. Conclusions. The study supports the idea that the decline of mental health in youth can seriously impact the psychological health of new generations of young adults.
Nutritional Status of Children Living with Nodding Disease Syndrome and Copying Strategies of Their Families in Uganda
Authors: Peter Enyagu , Bazira Joel , Kirungi Gloria 
Published: March 2022

Abstract
In northern Uganda, it is estimated that over 3,541 children in the districts of Kitgum, Pader and Lamwo were affected by nodding disease syndrome (NDS) by December 2013. The nodding Disease syndrome continues to manifest itself in Children in Akwang, Kitgum and other parts of Northern Uganda causing progressive cognitive dysfunction, neurological deterioration and stunted growth. This study sought to determine the nutritional status of children with NDS in Akwang Sub County, Kitgum District, identify the factors influencing this nutritional status and delineate the coping strategies at the household level. Methods: We used mixed methods, Children between 5–18 years with Nodding Disease Syndrome were chosen after their parents consented, and Akwang HCIII in Akwang sub county, Kitgum district was considered. This was majorly done for all children registered with the health Centre III and suffering from NDS, 123 children aged (5–18 years) were sampled and their parents/guardians were then traced using the snowballing method to their respective households from where consent was sought. They then participated by way of answering the questionnaire and taking of the anthropometric measurements of the children. We then used SPSS for analyzing quantitative data and Dedoose for qualitative data. Results: A total of 123 participants took part in the Study. Participants reported that prevalence of malnutrition in the NDS children is very high and this could be attributed to seizures while feeding, religious beliefs and age at weaning. Various coping mechanism include rearing animals, planting different kinds of crops for feeding and rearing cows and goats which are used as food to eat. This would play an important role in fighting malnutrition in NDS children in Northern Uganda. Conclusion: There is an urgent need to address the through factors that are associated with malnutrition among NDS children, and to strengthen the identified coping mechanisms. Empowering the care takers and prioritizing improvement on economic status could help in fighting malnutrition in NDS children.
Succession of forensically important Coleopterans from Southern Rajasthan: A preliminary study and their forensic relevance.
Authors: prasad arti , Meena Sanjay 
Published: March 2022

Abstract
Identification and knowledge of biology of insects is an initial and crucial step of using entomological evidence in forensic investigations. This preliminary study makes a strong platform for the importance of beetles (Order- Coloeptera) in forensic investigations. Methods: - The work reported here monitored the presence of the beetles by using bait traps with the goat meat (Capra sps. Bovidae) and laboratory mice, and also collected from decomposed animal bodies from various locations of different studied area. Result: - The main Coleopteran beetles collected from carcasses were Dermestes maculates (Dermestidae- carpet beetle), Necrobia rufipes (Cleridae- checkered beetle), and Saprinus planiusculus (Histeridae-clown beetle). Conclusion: - The Coleopteran insects studied here can be considered as strong clues for forensic investigations and also to detect the nature of crime with the help of biology and succession study of forensic insects.
The Exquisitely Preserved Dinosaur Skin of Psittacosaurus and the Scaly Skin of Ceratopsian Dinosaurs
Authors: Bell Phil , Hendrickx Christophe , Pittman Michael , Kaye Thomas , Mayr Gerald 
Published: March 2022

Abstract
The Frankfurt specimen of the early-branching ceratopsian dinosaur Psittacosaurus is remarkable for the exquisite preservation of squamous (scaly) skin and other soft tissues that cover almost its entire body. Newly detected details revealed under Laser-Stimulated Fluorescence (LSF) reveals the complexity of the squamous skin of Psittacosaurus, including several unique features and details of newly detected and previously-described integumentary structures. Variations in the scaly skin are found to be strongly regionalised in Psittacosaurus. For example, feature scales consist of truncated cone-shaped scales on the shoulder, but form a longitudinal row of quadrangular scales on the tail. Re-examined through LSF, the cloaca of Psittacosaurus has a longitudinal opening, or vent; a condition that it shares only with crocodylians. This implies that the cloaca had crocodylian-like internal anatomy, including a single, ventrally-positioned copulatory organ. Combined with these new integumentary data, a comprehensive review of integument in ceratopsian dinosaurs reveals that scalation was generally conservative in ceratopsians and typically consisted of large subcircular-to-polygonal feature scales surrounded by a network of smaller non-overlapping polygonal basement scales. This study highlights the importance of combining exceptional specimens with modern imaging techniques, which are helping to redefine the perceived complexity of squamation in ceratopsians and other dinosaurs.
The Medial Temporal Lobe Structure and Function Support Positive Affect
Authors: Jin Weipeng , Feng Jie , Zhu Wenwei , Zhang Bin , Chen Shuning , Wei Shiyu , Wang Pinchun , Deng Kun , Wang Yajie , Zhang Manman , Yang Shaofeng , Im Hohjin , WANG QIANG 
Published: March 2022

Abstract
Positive affect (PA) is not only associated with individuals’ psychological and physical health, but also their cognitive processes. However, whether medial temporal lobe (MTL) and its subfields’ volume/functional connectivity can explain individual variability in PA remains understudied. We investigated the morphological (i.e., gray matter volume; GMV) and functional characteristics (i.e., resting-state functional connectivity; rsFC) of PA combined with univariate and multivariate pattern analyses (MVPA) using a large sample of participants (n = 321). We simultaneously collected the T1-weighted (n = 321), high-resolution MTL T2-weighted, and resting-state functional imaging data (n = 209). The MTL and its subfields’ volumes, including the CA1, CA2 + 3, DG, and subiculum (SUB), perirhinal cortex (PRC), and parahippcampus (PHC), were extracted by automatic segmentation of hippocampal subfields (ASHS) software. The morphological results revealed that GMVs in the prefrontal-occipital and limbic (i.e., hippocampus, amygdala, and PHC) systems were associated with variability in PA at the whole-brain level using MVPA but not univariate analysis. Linear regression results further revealed a positive association between the MTL subfields’ GMV, especially for the right PRC, and PA after controlling for several covariates. PRC-seed-based rsFC analyses further revealed that its couplings with the fronto-parietal-occipital system predicted PA in both univariate and MVPA. These findings provide novel insights into the neuroanatomical and functional substrates underlying human PA trait. Findings also suggest critical contributions of the MTL and its subfield of the perirhinal cortex, but not hippocampal subfields, as well as its functional coupling with the fronto-parietal control-system on the formation of PA.
Self-cleaning and air de-pollution performance of TiO2/SiO2 and N-TiO2/SiO2 photocatalysts coated on Portland cement mortar substates
Authors: Khannyra Souad , Gil Maria Luisa Almoraima , Addou Mohammed , Mosquera Maria.J. 
Published: March 2022

Abstract
In this study, the newly synthesized TiO2 and N doped TiO2 clusters were added to silica sol to synthesize N-TiO2/SiO2 composites via the sol-gel method. Afterwards, the prepared sols were applied by brushing on portland cement. Doping with nitrogen significantly increased the absorption of TiO2 towards the visible region, thus, increasing the photocatalytic activity. SEM characterization of the treated samples showed that the clusters were distributed in form of aggregates on the ssamples' surface. The self-cleaning and air de-polluting performances were assessed through methylene blue degradation and the oxidation of nitrogen oxide, resulting in methylene blue (MB) removal of 85% and 78% after 60 min of irradiation for SN10TiO2 and STiO2, respectively. Regarding air de-pollution performance, the newly synthesized photocatalysts showed the ability of NOx reduction. However, their efficiency was somewhat lower, in which 23.81% of NO has been oxidized by the sample SN10TiO2, while SP25 showed a total NO conversion of 38.98%. The powdered xerogels of the newly synthesized nanoparticles revealed high photocatalytic efficiency concerning NO oxidation, resulting in higher performance compared to those obtained by the xerogel containing P25.
A machine learning route between band mapping and band structure
Authors: Xian Rui Patrick , Stimper Vincent , Zacharias Marios , Dong Shuo , Dendzik Maciej , Beaulieu Samuel , Schoelkopf Bernhard , Wolf Martin , Rettig Laurenz , Carbogno Christian , Bauer Stefan , Ernstorfer Ralph 
Published: March 2022

Abstract
Electronic band structure (BS) and crystal structure are the two complementary identifiers of solid state materials. While convenient instruments and reconstruction algorithms have made large, empirical, crystal structure databases possible, extracting quasiparticle dispersion (closely related to BS) from photoemission band mapping data is currently limited by the available computational methods. To cope with the growing size and scale of photoemission data, we develop a pipeline including probabilistic machine learning and the associated data processing, optimization and evaluation methods for band structure reconstruction, leveraging theoretical calculations. The pipeline reconstructs all 14 valence bands of a semiconductor and shows excellent performance on benchmarks and other materials datasets. The reconstruction uncovers previously inaccessible momentum-space structural information on both global and local scales, while realizing a path towards integration with materials science databases. Our approach illustrates the potential of combining machine learning and domain knowledge for scalable feature extraction in multidimensional data.
Impact of prostatic shape on the difficulty of robot-assisted laparoscopic radical prostatectomy
Authors: Kamisawa Ken , Matsumoto Kazuhiro , Takeda Toshikazu , Takamatsu Kimiharu , Niwa Naoya , Yasumizu Yota , Tanaka Nobuyuki , Morita Shinya , Kosaka Takeo , Mizuno Ryuichi , Asanuma Hiroshi , Oya Mototsugu 
Published: March 2022

Abstract
BackgroundTo investigate the impact of prostatic shape observed on preoperative magnetic resonance imaging (MRI) on the difficulty of robot-assisted laparoscopic radical prostatectomy (RALP).MethodsWe retrospectively reviewed the operative records of 211 patients who underwent RALP. We excluded patients who received neoadjuvant therapy. All surgeries in this study were performed by two surgeons. Each patient clinicopathological and surgical data were reviewed. Prostate sphericity was evaluated by measuring the roundness of the prostate at the largest axial slice by MRI. The console time was adopted as an objective indicator for assessing surgical difficulty.Results The mean prostate volume was 34 cc (range 14 to 88) and the mean prostate roundness was 0.55 (range 0.24 to 0.90). The mean console time was 194 minutes (range 95 to 296). To assess the relationship between prostate volume and console time, scatter plot analysis was performed. The prostate volume had a weak positive correlation with the console time (r =0.165, p=0.016). Similarly, scatter plot analysis between the prostate roundness and console time demonstrated a weak positive correlation (r =0.167, p=0.015) . Next, we performed subgroup analysis of 56 patients with a large prostate volume (≥ 40 cc), and the positive correlation between the prostate volume and the console time disappeared (r =0.142, p=0.296). On the other hand, the prostate roundness was more strongly correlated with the console time (r =0.439, p=0.001).ConclusionsOur results indicated that the spherical shape of the prostate is associated with the surgical difficulty of RALP, especially in patients with a large prostate volume.
Application of high-throughput, high-depth, targeted single-nucleus DNA sequencing in
pancreatic cancer
Authors: Zhang Haochen , Karnoub Elias-Ramzey , Umeda Shigeaki , Chaligné Ronan , Masilionis Ignas , McIntyre Caitlin , Hayashi Akimasa , Sashittal Palash , Zucker Amanda , Mullen Katelyn , Makohon-Moore Alvin , Iacobuzio-Donahue Christine 
Published: March 2022

Abstract
Despite insights gained by bulk DNA sequencing of cancer it remains challenging to resolve the admixture of normal and tumor cells, and/or of distinct tumor subclones; high throughput single-cell DNA sequencing circumvents these and brings cancer genomic studies to higher resolution. However, its application has been limited to liquid tumors or a small batch of solid tumors, mainly because of the lack of a scalable workflow to process solid tumor samples. Here we optimized a highly automated nuclei extraction workflow that achieved fast and reliable targeted single-nucleus DNA library preparation of 38 samples from 16 pancreatic adenocarcinoma (PDAC) patients, with an average library yield per sample of 2867 single nuclei. We demonstrate that this workflow not only performs well using low cellularity or low tumor purity samples but reveals novel genomic evolution patterns of PDAC as well.
The Effect of Ambient PM2.5 Exposure on Survival of Lung Cancer Patients After Lobectomy
Authors: Liu Changpeng , Yang Dongjian , Liu Yuxi , Piao Heng , Zhang Tao , Li Xi , Zhao Erjiang , Zhang Di , Zheng Yan , Tang Xiance 
Published: March 2022

Abstract
Exposure to fine particulate matter (PM2.5) is linked to lung cancer incidence and mortality. However, the impact of PM2.5 exposure on lung cancer patients after lobectomy, which remains the primary treatment for early-stage lung cancer, is unknown. Therefore, we investigated the correlation between PM2.5 exposure and survival of lung cancer patients after lobectomy. This study included 3,327 patients with lung cancer who underwent lobectomy. We converted residential addresses into coordinates and estimated individual patients' daily PM2.5 and O3 exposure levels. The Cox multivariate regression model was used to analyze the specific monthly association between PM2.5 exposure and lung cancer survival. Every 10 μg/m3 increase in monthly PM2.5 concentration in the first and second months after lobectomy increased the risk of death (hazard ratio [HR]: 1.043, 95% confidence interval [CI]: 1.019–1.067 and HR: 1.036, 95% CI: 1.013–1.060, respectively). Non-smokers, younger patients, and patients with longer hospitalization durations had worse survival when exposed to greater concentrations of PM2.5. High postoperative PM2.5 exposure immediately after lobectomy reduced the survival of patients with lung cancer. Patients should be transferred after lobectomy to areas with better air quality to prolong their survival time.
Effects of Complex Spa Therapy in Patients with Osteoarthritis of the Spine Receiving Treatments in Health Resorts in South-Eastern Poland
Authors: Zwolińska Jolanta , Gąsior Monika 
Published: March 2022

Abstract
Management of patients with degenerative diseases commonly comprises health-resort based treatment programs, including spa therapies, balneotherapy as well as terrain therapy making use of microclimate factors. The study was designed to assess short- and long-term effects of spa therapy administered to patients with osteoarthritis of the spine who received treatment in health resorts located in Poland. The study involved 102 patients receiving treatment in health resorts, a group of patients receiving ambulatory treatment (100 patients) and a group receiving no therapy (100 patients). The assessment survey included: Pain VAS and Laitinen, LISAT-9 and HAQ-20 questionnaires. The assessments were carried out three times: at the start of the therapy program, as well as one month and six months after the end of the program. Short-term effects showed statistically significant improvement in all the outcome measures in spa group and outpatient treatment group. The long-term effects showed statistically significant improvement in all the outcome measures in spa group only. In conclusion spa therapy reduces pain, improves functional efficiency and increases the level of life satisfaction in patients with osteoarthritis of the spine. Its effects are sustained for at least six months. Spa therapy is more effective long-term, than outpatient treatment. The study was registered at Clinical Trials: NCT03974308.The study was approved by the Ethics Commission of the University of Rzeszów (Resolution No. Nr 7/04/2019 dated 11/04/2019). Fully anonymous survey was conducted.
Loss of SUMO-specific protease 2 causes isolated glucocorticoid deficiency by blocking adrenal cortex zonal transdifferentiation
Authors: Dufour Damien , Dumontet Typhanie , Sahut-Barnola Isabelle , Onzon Méline , Pussard Eric , Wilmouth James , Olabe Julie , Lucas Cécily , Levasseur Adrien , Damon-Soubeyrand Christelle , Pointud Jean-Christophe , Roucher-Boulez Florence , Tauveron Igor , Bossis Guillaume , Yeh Edward , Breault David , VAL Pierre , Lefrançois-Martinez Anne-Marie , Martinez Antoine 
Published: March 2022

Abstract
SUMOylation is a dynamic posttranslational modification, that provides fine-tuning of protein function involved in the cellular response to stress, differentiation, and tissue development. In the adrenal cortex, an emblematic endocrine organ that mediates adaptation to physiological demands, the SUMOylation gradient is inversely correlated with the gradient of cellular differentiation raising important questions about its role in functional zonation and the response to stress. Considering that SUMO-specific protease 2 (SENP2), a deSUMOylating enzyme, is upregulated by ACTH/PKA signalling within the zona Fasciculata (zF), we generated mice with adrenal-specific Senp2 loss to address these questions. Disruption of SENP2 activity in steroidogenic cells leads to specific hypoplasia of the zF, a blunted reponses to ACTH and isolated glucocorticoid deficiency. Mechanistically, overSUMOylation resulting from SENP2 loss shifts the balance between ACTH/PKA and WNT/β-catenin signalling leading to repression of PKA activity and ectopic activation of β-catenin. At the cellular level, this blocks transdifferentiation of β-catenin-positive zona Glomerulosa cells into zF cells and sensitises them to premature apoptosis. Our findings indicate that the SUMO pathway is critical for adrenal homeostasis and stress responsiveness.