bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hubungan Status Gizi dengan Usia Menarche
Authors: Enggar Enggar , Suastuti Ni Putu , Rosiyana Ni Made 
Published: June 2022

Introduction: Early menarche age is associated with an increase in body mass index. Nutrient intake also affects the sexual maturity of adolescent girls. Adolescent girls who get early menarche tend to have more weight and height than those who have not. The purpose of this study was to determine the relationship between nutritional status and age of menarche at SMP Negeri 6 Palu. Methods: This study uses a cross sectional approach, a sample of 45 people from March to July 2020 using purposive sampling technique, measuring nutritional status using BMI and data analysis using chi square. The results showed that there was no relationship between nutritional status and age at menarche, p-value 0.542. The age at which menarche occurs is not affected by either being thin, normal or obese. It is advisable for young women to eat a balanced diet to maintain a healthy nutritional status during menarche. Inadequate nutrition will interfere not only with growth and organ function, but also reproductive function, especially menstrual disorders.

ABSTRAK
Pendahuluan: Cepatnya usia Menarche dikaitkan dengan meningkatnya Indeks Masa Tubuh. Asupan zat gizi juga mempengaruhi kematangan seksual remaja putri. Remaja putri yang mendapatkan Menarche dini cenderung memiliki berat badan dan tinggi badan yang lebih dibandingkan dengan yang belum Menarche. Tujuan penelitian ini untuk mengetahui hubungan status gizi dengan usia Menarche di SMP Negeri 6 Palu. Metode: Penelitian ini menggunakan pendekatan cross sectional, sampel 45 orang pada bulan Maret sampai Juli 2020 menggunakan teknik purposive sampling, pengukuran status gizi menggunakan IMT dan analisis data menggunakan chi square. Hasil penelitian didaptkan bahwa tidak ada hubungan antara status gizi dengan usia menarche p-value 0,542. usia menarche terjadi tidak dipengaruhi baik dari kurus, normal atau gemuknya seseorang. Sebaiknya remaja putri mengonsumsi makanan dengan gizi seimbang untuk menjaga status gizi yang sehat selama menarche. Gizi yang tidak adekuat akan mengganggu tidak hanya pertumbuhan dan fungsi organ tubuh, tetapi juga fungsi reproduksi terutama gangguan mentrusi.
Effects of a Dried Neem Leaf Extract on the Growth Performance, Meat Yield and Meat Quality in Skeletal Muscle of Broiler Chickens Under High-Temperature Conditions
Authors: Nakamura Kiriko , Katafuchi Ayumi , Shimamoto Saki , Ogawa Goichiro , Khandelwal Nikhil , Tatsugawa Kenji , Fujita Yoshikazu , Ohtsuka Akira , Ijiri Daichi 
Published: June 2022

We aimed to examine the effects of cyclical high ambient temperature (HT) and dried Neem (Azadirachta indica) leaf extract (DNE) supplementation on the growth performance, muscle lipid peroxidation level, and muscle drip loss of broiler chickens. Twenty-four 15-day old broiler chickens (Chunky strain ROSS 308) were divided into four treatment groups that were fed diets with or without 2.0% DNE under thermoneutral (25 ± 1°C) or cyclical HT (35 ± 1°C for 8 h/day) conditions. Supplementation of DNE did not affect the growth performance of the chicks, but HT reduced their feed intake, the weights of breast muscle and heart. In addition, supplementation with DNE ameliorated the negative effects of cyclical HT on feed intake and breast muscle mass. Furthermore, cyclical HT increased the malondialdehyde (MDA) concentration and drip loss over 48 h of storage of the breast muscle, and these effects were ameliorated by DNE. Collectively, we conclude that dietary supplementation with DNE reduces the muscle MDA concentration and drip loss of broiler chickens kept under HT conditions.
Combinatorial CRISPR Interference Library for Enhancing 2,3-BDO Production and Elucidating Key Genes in Cyanobacteria
Authors: Li Hung , Pham Nam Ngoc , Shen Claire R. , Chang Chin-Wei , Tu Yi , Chang Yi-Hao , Tu Jui , Nguyen Mai Thanh Thi , Hu Yu-Chen 
Published: June 2022

Cyanobacteria can convert CO2 to chemicals such as 2,3-butanediol (2,3-BDO), rendering them promising for renewable production and carbon neutralization, but their applications are limited by low titers. To enhance cyanobacterial 2,3-BDO production, we developed a combinatorial CRISPR interference (CRISPRi) library strategy. We integrated the 2,3-BDO pathway genes and a CRISPRi library into the cyanobacterium PCC7942 using the orthogonal CRISPR system to overexpress pathway genes and attenuate genes that inhibit 2,3-BDO formation. The combinatorial CRISPRi library strategy allowed us to inhibit fbp, pdh, ppc, and sps (which catalyzes the synthesis of fructose-6-phosphate, acetyl-coenzyme A, oxaloacetate, and sucrose, respectively) at different levels, thereby allowing for rapid screening of a strain that enhances 2,3-BDO production by almost 2-fold to 1583.8 mg/L. Coupled with a statistical model, we elucidated that differentially inhibiting all the four genes enhances 2,3-BDO synthesis to varying degrees. fbp and pdh suppression exerted more profound effects on 2,3-BDO production than ppc and sps suppression, and these four genes can be repressed simultaneously without mutual interference. The CRISPRi library approach paves a new avenue to combinatorial metabolic engineering of cyanobacteria.
Enhanced Glial Reaction and Altered Neuronal Nitric Oxide Synthase are Implicated in Attention Deficit Hyperactivity Disorder
Authors: Zhang Peng , Fang Huyue , Lou Chengjian , Ye Shan , Shen Guanghong , Chen Shijia , Amin Nashwa , Botchway Benson O. A. , Fang Marong 
Published: June 2022

Attention deficit hyperactivity disorder (ADHD) has a complex etiology, and its specific causal factors remain to be elucidated. Aberration of nitric oxide synthase (nNOS) and inflammation, together with astrocytic and microglial cells have been continually associated with several neurological disorders, including ADHD. Using spontaneously hypertensive rat (SHR), we investigated the changes in nNOS, inflammatory, microglial and astrocytic markers in the frontal cortex and hippocampus at three different ages: onset of hypertension stage (i.e., 6 weeks after birth of SHR), established hypertension stage (i.e., 12 weeks after birth of SHR) and senescent stage (i.e., 12 months after birth of SHR), and compared with its age-matched normotensive control, Wistar-Kyoto (WKY) rats. A significant upregulation of Iba-1 expression in the senescent stage of SHR was observed. Further, we observed an upregulated nNOS expression in both onset and established stages of SHR, and a downregulated nNOS in the senescent stage. Our study showed an age-related increment of astrogliosis in the cortex and hippocampi of aged SHR. On the basis of our results, alterations in the nNOS and Iba-1 expressions, as well as age-related astrogliosis, may contribute to ADHD pathogenesis.
Associations of Clinical Factors and Blood Groups With the Severity of COVID-19 Infection in Makkah City, Saudi Arabia
Authors: Shesha Nashwa , Melebari Sami , Alghamdi Saad , Refaat Bassem , Naffadi Hind , Alquthami Khalid 
Published: June 2022

ContextThe possible associations between the different blood groups and clinical factors with COVID-19 infection among patients in Makkah city.ObjectiveTo investigate the relationship between ABO blood groups and COVID-19 infection in patients who were tested positive and to elucidate the most common ABO blood groups with a higher infectivity of COVID-19 and disease association.Materials and MethodsThis was an observational cross-sectional study that included COVID-19 patients diagnosed with PCR and who were hospitalized in Al-Noor Specialist Hospital (Makkah) during the period between March to November 2020. The ABO and Rhesus blood groups alongside the clinical characteristics were determined and retrieved from medical records and HESN of the Ministry of Health of the Kingdom of Saudi Arabia (KSA).ResultsThe overall confirmed COVID-19 cases included in this study were 1,583 patients who underwent positive PCR testing between March and November 2020. The frequencies of blood groups were as follows: group O+ (37%), group A+ (29.2%), group B+ (22.6%), group AB+ (5.1%), group O- (2.8%), group B- (1.8%), group A- (1.1%), and group AB- (0.4%). However, no significant correlations were observed for ABO groups and Rh types with the severity of COVID-19 illness. Conversely, signs and symptoms of respiratory distress syndrome (RDS), pneumonia, and respiratory failure symptoms, alongside a history of diabetes mellitus, hypertension, chronic kidney diseases, and congestive heart failure significantly increased the risk of death from COVID-19 infection. Moreover, the rates of fever, cough, and asthma were markedly lower in the deceased group compared with the recovered group of patients.ConclusionThe association between the different blood groups with the prevalence and mortality of COVID-19 among infected patients has yet to be elucidated as we found no significant differences in the observed versus expected distribution of ABO phenotypes among the included cases. The prevalence of RDS, pneumonia, and respiratory failure was found higher among hospitalized COVID-19 patients in the deceased group. However, other factors such as fever, cough, and asthma appeared to be more significantly lower than in the recovered group.
Changes in Impaired Fasting Glucose and Borderline High Low-Density Lipoprotein-Cholesterol Status Alter the Risk of Cardiovascular Disease: A 9-Year Prospective Cohort Study
Authors: Wang Xianxuan , Zhou Yan-Feng , Huang Zegui , Yu Xinran , Chen Zekai , Cai Zefeng , Lan Yulong , Li Werijian , Cai Zhiwei , Fang Wei , Chen Guanzhi , Wu Weiqiang , Wu Shouling , Chen Youren 
Published: June 2022

BackgroundWe aimed to characterize the relationships of the changes in impaired fasting glucose (IFG) and borderline high low-density lipoprotein-cholesterol (LDL-C) status with cardiovascular disease (CVD).MethodsA total of 36,537 participants who did not have previous CVD, diabetes mellitus, or high LDL-C (≥ 4.1 mmol/L), nor were taking lipid-lowering drugs were recruited from the Kailuan study. The participants were allocated to six groups according to their baseline and follow-up fasting blood glucose (FBG) and LDL-C concentrations: (1) both were normal; (2) both normal at baseline, one abnormality subsequently; (3) both normal at baseline, both abnormal subsequently; (4) at least one abnormality that became normal; (5) at least one abnormality at baseline, a single abnormality subsequently; and (6) at least one abnormality, two abnormalities subsequently. The outcomes were CVD and subtypes of CVD (myocardial infarction and stroke). Multiple Cox regression models were used to calculate adjusted hazard ratio (HR) and confidence interval (95% CI).ResultsDuring a median follow-up period of 9.00 years, 1,753 participants experienced a CVD event. After adjustment for covariates, participants with IFG in combination with a borderline high LDL-C status at baseline and follow-up had higher risks of CVD (HR: 1.52; 95% CI: 1.04–2.23 and HR: 1.38, 95% CI: 1.13–1.70, respectively) compared with those with normal fasting blood glucose and LDL-C. Compared with participants that remained normal, those who changed from normality to having two abnormalities were at a higher risk of CVD (HR: 1.26; 95% CI: 0.98–1.61), as were those who changed from at least one abnormality to two abnormalities (HR: 1.48, 95% CI: 1.02–2.15).ConclusionChanges in IFG and borderline high LDL-C status alter the risk of CVD and its subtype, implying that it is important to focus on such individuals for the prevention and control of CVD.
Aquifer-Peatland Hydrological Connectivity and Controlling Factors in Boreal Peatlands
Authors: Lambert C. , Larocque M. , Gagné S. , Garneau M. 
Published: June 2022

The conditions in which groundwater inflow occurs in boreal peatlands and its contribution to peatland water balance are still poorly understood. The objectives of this research were to quantify the hydrological connectivity between a surficial aquifer and a peatland, and to identify the controlling factors in boreal peatlands of north-central Quebec (Canada). The peatlands were instrumented with piezometers and groundwater levels were monitored during two growing seasons. Hydraulic conductivities were measured on peat cores andin situ,groundwater inflows and outflows were calculated using the Darcy equation. The peatland water budgets were simulated for the two peatlands with a steady-state groundwater flow model to verify flow hypotheses, to quantify unmeasured flows and to explore recharge scenarios leading to changes in connectivity. The two peatlands have contrasted water budgets, with recharge representing the largest inflow (78%) and subsurface runoff representing the largest outflow (85%) the peatland with the smallest catchment area (Misask). The peatland with the largest catchment area (Cheinu) is also located downgradient within the regional watershed. Its inflows are dominated by groundwater (56%) and its outflows are mostly towards subsurface runoff (74%). The two peatlands are in conditions of precipitation excess and a recharge reduction would not affect their peatland heads markedly (&lt;10 cm). However, recharge changes could induce larger modifications in groundwater inflows and outflows for the peatland with a larger catchment area. The dominating peatland hydrological functions are thus contrasted at the two sites, and it is hypothesized that the water table depths thresholds triggering changes between storage, transmission and runoff functions are also different. Although further studies remain to be done to understand how hydrological conditions change through time, and ultimately what are the long-term impacts of a changing climate on hydrology, vegetation and carbon accumulation, this work shows that understanding peatland hydrology requires to consider hydrological conditions beyond the peatland limits.
Long-Term Trends in Black Carbon and Aerosol Optical Depth Over the Central Himalayas: Potential Causes and Implications
Authors: Joshi Hema , Naja Manish , Srivastava Priyanka , Gupta Tarun , Gogoi Mukunda M. , Suresh Babu S. 
Published: June 2022

Continuous ground-based observations of aerosols over the Himalayan region are highly beneficial in studying aerosol trends over the complex geographic terrain. This study presents the results from continuous long-term observations (2004–2016) of aerosols over a high-altitude site in the central Himalayas – Nainital, quantifying multihued aspects of black carbon (BC) and columnar aerosol optical depth (AOD). The results reveal that BC and AOD have a positive trend of 23.04 ± 3.00 ng m−3 year−1 and 0.0076 ± 0.0003 year−1, respectively. Simultaneously, a positive trend of 0.0066 ± 0.0059 year−1 is observed in the Angstrom exponent, thus indicating an increase in anthropogenic aerosols. The satellite observations covering a broader region around Nainital also depicted increasing trends in AOD (0.0042–0.0074 year−1), absorbing aerosol optical depth (0.0001 ± 0.0001 year−1) and aerosol index (0.0131 ± 0.0019 year−1), along with a slight decline in single scattering albedo (−0.0004 year−1). These results suggest that aerosols of absorbing nature might have increased over this region in recent times. Investigation of the AOD ratio, obtained from aerosol vertical profiles, revealed an increasing trend in aerosol load below 1 km. The observed trends in aerosols are found to be associated with enhanced biomass burning activity, reflected in terms of the positive trend in fire count ∼38.65 ± 5.88 year−1 over the region.
Discovering a Four-Gene Prognostic Model Based on Single-Cell Data and Gene Expression Data of Pancreatic Adenocarcinoma
Authors: Huang Weizhen , Li Jun , Zhou Siwei , Li Yi , Yuan Xia 
Published: June 2022

BackgroundPancreatic cancer has a 5-year overall survival lower than 8%. Pancreatic adenocarcinoma (PAAD) is the most common type. This study attempted to explore novel molecular subtypes and a prognostic model through analyzing tumor microenvironment (TME).Materials and MethodsSingle-cell RNA sequencing (scRNA-seq) data and expression profiles from public databases were downloaded. Three PAAD samples with single-cell data and 566 samples with gene expression data were included. Seurat was used to identify cell subsets. SVA merged and removed batch effects from multichip datasets. CIBERSORT was used to evaluate the components of different cells in transcriptome, ConsensusClusterPlus was used to identify molecular subtypes, and gene set enrichment analysis was used for functional enrichment analysis. LASSO Cox was performed to construct dimensionality reduction and prognosis model.ResultsMemory B cells (MBCs) were identified to be significantly with PAAD prognosis. Two immune subtypes (IS1 and IS2) with distinct overall survival were constructed. Forty-one DEGs were identified between IS1 and IS2. Four prognostic genes (ANLN, ARNTL2, SERPINB5, and DKK1) were screened to develop a prognostic model. The model was effective in classifying samples into high-risk and low-risk groups with distinct prognosis. Three subgroups of MBCs were identified, where MBC_0 and MBC_1 were differentially distributed between IS1 and IS2, high-risk and low-risk groups.ConclusionsMBCs were closely involved in PAAD progression, especially MBC_0 and MBC_1 subgroups. The four-gene prognostic model was predictive of overall survival and could guide immunotherapy for patients with PAAD.
Global Trends in Research of Androgen Receptor Associated With Breast Cancer From 2011 to 2020: A Scientometric Analysis
Authors: Chen Lingzhi , Liu Yiyuan , Cai Jiehui , Ji Zeqi , Zou Juan , Chen Yaokun , Wu Jinyao , Zheng Daitian , Zheng Jiehua , Chen Yexi , Li Zhiyang 
Published: June 2022

Recently, the androgen receptor has been found as a potential prognostic index and therapeutic target for breast cancer. To reveal the current research status and hotspots in this area, we analyzed the characteristics of related publications from 2011 to 2020. All related publications from 2011 to 2020 were retrieved from the Web of Science. Biblioshiny, VOSviewer, and CiteSpace V were applied to obtain the information on annual publications and citations, the highest yielding countries and authors, influential journals and articles, as well as hot keywords. In total, 2,118 documents, including 1,584 original articles and 534 reviews, were retrieved. Annual publication output was rich from 2014 to 2018, reaching the top in 2017. A systematic review written by Lehman et al. in 2011 was the most-cited document and reference. The United States was the leading country with the maximum number of publications, citations, and link strengths with other countries. The journal publishing the most was Oncotarget. Lehmann was the author who had the highest link strengths with other authors. The most highlighted keywords were “androgen receptor” (n = 1,209), “breast cancer” (n = 690), “expression” (n = 545), “breast cancer” (n = 410), “prostate cancer” (n = 290), and so on, revealing the trend from molecular mechanism level to therapeutic use level. The androgen receptor plays a significant role in the development of breast cancers, whereas its therapeutic value seems to be controversial and needs further study. With the help of a scientometric analysis in this field, researchers can clarify the current research status and hotspots worth fully exploring.
Tell Us a Story Granddad: Age and Origin of an Iconic Australian Lungfish
Authors: Mayne Benjamin , Espinoza Tom , Roberts David 
Published: June 2022

The modern discovery of the Australian lungfish (Neoceratodus forsteri) by European settlers in 1870 was considered one of the most important events in natural history by leading international scientists and naturalists of that time. Its distinct evolutionary lineage and unique extant morphological characteristics fostered the romantic zoological moniker “living fossil”. Although N. forsteri were suspected of being long-lived, a reliable estimate of maximum age has remained elusive. Maximum age is critical to inform wildlife management and conservation efforts, including the use of population viability models. To estimate the maximum age for N. forsteri, we sourced DNA from “Granddad”, the presumed longest-living lungfish known in a zoological park and utilised an epigenetic ageing clock developed for N. forsteri. This lungfish specimen was gifted to the Chicago John G. Shedd Aquarium from Australia in 1933 and lived there for 84 years until death in 2017. We estimated the age of Granddad at death to be 109 years (±6 years), confirming N. forsteri as a true centenarian species. Genotyping also revealed the natal origin of Granddad to be the Burnett River, Queensland, Australia, the location of the species’ original discovery in 1870. We demonstrate the application of novel molecular techniques to a unique long-lived and captive-raised specimen, to improve estimates of maximum age for the species, and to identify natal origin. This information will support future conservation efforts for this iconic yet endangered species.
Adsorption Characteristics of Three Types of Soils on Biogas Slurry Ammonium Nitrogen
Authors: Wang Zichen , Zhang Liping , Sun Guofeng , Zhou Wei , Sheng Jing , Ye Xiaomei , Olaniran Ademola O. , Kana E. B. Gueguim 
Published: June 2022

Using farmland to digest biogas slurry is an effective measure to overcome the bottleneck of sewage treatment in livestock and poultry farms. However, there is limited research on the soil adsorption characteristics of biogas slurry ammonium nitrogen (NH4+-N). In addition, the maximum adsorption capacity (Qm) of farm soil is unclear. In this study, three typical farmland tillage layer soils (silty loam, loam, and sandy loam) were used to analyze adsorption characteristics through adsorption kinetics experiments (adsorption for 0.25, 0.5, 1, 2, 4, 6, 12, 18, or 24 h with NH4+-N concentrations of 42.90 mg/L) and thermodynamic experiments (adsorption for 3 days with NH4+-N concentrations of 54.25, 88.66, 105.85, 133.71, 178.80, 273.54, and 542.87 mg/L). The Qm value was fitted by models, and its relationship with soil properties was discussed. The results showed the following: 1) the adsorption of biogas slurry NH4+-N by the three types of soils was a composite kinetic process that comprised two stages of rapid and slow reactions. Rapid adsorption predominantly occurred within 0–1 h, and the adsorption capacity accounted for 35.24%–43.55% of the total adsorption. The ExpAssoc equation produced a good fit for the adsorption kinetic behavior in the three soil types. 2) The equilibrium adsorption could be described by the Langmuir equation, the Freundlich equation, the PlPlatt model, and the Langevin model isotherm, among which the Langevin model had the best fit, with a coefficient of determination R2 close to 1. The theoretical saturated Qm fitting results of NH4+-N were 1038.41–1372.44 mg/kg in silty loam, 840.85–1157.60 mg/kg in loam, and 412.33–481.85 mg/kg in sandy loam. The optimal values were 1108.55, 874.86, and 448.35 mg/kg for silty loam, loam, and sandy loam, respectively. 3) The Qm value was significantly positively correlated with soil organic matter, total nitrogen, available phosphorus, available potassium, cation exchange capacity, and particle content of 0.02–0.002 mm (p &lt; 0.01), but significantly negatively correlated with soil pH (p &lt; 0.05). This study can provide a reference for the safe application of biogas slurry on farmland.