bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers to Adequate Pain Control and Opioid Use Among Cancer Survivors: Implications for Nursing Practice
Authors: Marshall Victoria Kate , Chavez Melody , Efre Andrea , Lake Paige W. , Rigg Khary K. , Lubrano Barbara , Pabbathi Smitha , Rajasekhara Sahana , Tyson Dina Martinez 
Published: May 2022

Background
Cancer survivors can experience long-term negative effects from cancer and its treatment. Pain is one of the most common and distressing symptoms that cancer survivors experience. Opioids are often prescribed for pain; however, cancer survivors who have completed active treatment may have unique challenges with regard to pain management.

Objective
The aim of this study was to explore barriers to pain management and perceptions of opioid use among cancer survivors.

Methods
This research was an exploratory pilot study using in-depth qualitative interviews with adult cancer survivors who were recruited from community-based survivorship organizations. Data were analyzed using applied thematic analysis techniques.

Results
Participants (n = 25) were mostly women (96%), diagnosed with breast cancer (88%) and stages I to III disease (84%), with a mean age of 56.2 years. Three themes on barriers to adequate pain control emerged: (1) taking just enough to take the edge off: self-medicating behaviors and nonadherence to prescribed regimen; (2) lack of insurance coverage and costly alternative pain treatment options; and (3) chronicity of cancer-related pain not adequately addressed and often mismanaged.

Conclusions
Discussions with cancer survivors unveiled personal accounts of unmanaged pain resulting from limited pain management/opioid education, fear of opioid addiction, negative perceptions/experiences with opioids, lack of insurance coverage for alternative pain therapies, and regulatory policies limiting access to opioids.

Implications for Practice
There is a clear need for improved access to multimodal pain management options and nonopioid alternatives for cancer survivors. Oncology nurses should endeavor to support policies and procedures aimed at opioid education, training, and legislation.
Increasing Racial and Ethnic Diversity in the Nursing Workforce
Authors: Hinson Tyonne D. , Brostoff Marcie , Grossman Amanda Beit , Ward Valerie L. , Lind Kathleen , Wood Laura J. 
Published: May 2022

Abstract

Background:
The 2021 Future of Nursing Report 2020-2030: Charting a Path to Achieve Health Equity recognizes increasing racial and ethnic diversity in nursing as an imperative to achieving health equity.

Practice Initiatives:
Over a 3-year period, nursing and human resource leaders at Boston Children's Hospital, a tertiary care, 454-bed pediatric academic medical center in Massachusetts, developed, implemented, and evaluated specific strategies to increase racial and ethnic diversity in recruitment and hiring of the nursing workforce. These specific strategies focused on cultivating partnerships, building relationships with candidates, and supporting transition into practice.

Results:
Significant increases in racial and ethnic diversity recruitment and hiring were achieved over the 3-year period. In 2019, strategies yielded a 6% overall increase in total registered nurse diversity hiring with an 18% increase in new graduate diversity hires over 2018. In total, 16.2% of registered nurse hires for 2019 were racially and ethnically diverse. Subsequent years yielded similar success in the recruitment of diverse registered nurses.

Clinical Implications:
With the projected growth of racial and ethnic minority populations, nursing and health care leaders must prioritize intentional strategic diversity recruitment and retention actions to address this imperative to advance health equity through the creation of a racially and ethnically diverse nursing workforce.
The Initial Approach to the Multisystem Pediatric Trauma Patient
Authors: Hannon Megan M. , Middelberg Leah K. , Lee Lois K. 
Published: May 2022

Abstract
Trauma remains the leading cause of morbidity and mortality in children and youth 1 to 19 years old in the United States. Providing timely care with a systematic approach is essential for emergently addressing life-threatening injuries and ongoing assessment. The primary survey is focused on identifying and managing life-threatening injuries. The secondary survey is focused on identifying and managing other important injuries. Over the past decade, there have been important advances in the evidence supporting the management of multisystem trauma in the pediatric patient by the emergency medicine clinician. In addition, the emergence of diagnostics, such as point-of-care ultrasound, aids decision making in the evaluation and management of the pediatric trauma patient. The purpose of this article is to review the initial systematic diagnostic approach and the emergent management of multisystem injuries from blunt force trauma in children in the emergency department and provide insight into the aspects of care that are still evolving.
Thalassolituus alkanivorans sp. nov., a hydrocarbon-utilizing bacterium isolated from the Mariana Trench
Authors: Wei Ting-Ting , He Shan , Quan Zhe-Xue 
Published: May 2022

Two strains, TMPB967T and TTPB476, were isolated from two different locations in the Mariana Trench. Cells of strains TMPB967T and TTPB476 were Gram-negative, curved rod-shaped (0.35–0.6 µm×2–4 µm) with flagella. Both strains were catalase- and oxidase-positive. Strains TMPB967T and TTPB476 could grow at 4–37 °C (optimum, 37 °C), at pH 6–9 (optimum, pH 6–7) and in the presence of 0–8 % (w/v) NaCl (optimum, 5 %). Both strains could grow with tetradecane or hexadecane as the sole carbon source. The predominant isoprenoid quinone was ubiquinone 9. The major cellular fatty acids of strains TMPB967T and TTPB476 were C18 : 1
ω9c, C16 : 0 and summed feature 3 (C16 : 1
ω7c or ω6c). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminolipid. The DNA G+C contents of strains TMPB967T and TTPB476 were 53.1 and 53.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the most closely related validly published species were

Thalassolituus marinus

IMCC1826T (97.1 % similarity) and

Thalassolituus oleivorans

MIL-1T (95.9 % similarity). Digital DNA–DNA hybridization results of strain TMPB967T with TTPB476,

T. marinus

IMCC1826T and

T. oleivorans

MIL-1T were 99.9, 20.9 and 20.2 %, respectively. Average nucleotide identity results of strain TMPB967T with TTPB476,

T. marinus

IMCC1826T and

T. oleivorans

MIL-1T were 100, 75.8 and 72.0 %, respectively. On the basis of the phenotypic, chemotaxonomic and molecular features, strains TMPB967T and TTPB476 belong to a novel species within the genus

Thalassolituus

, for which the name Thalassolituus alkanivorans sp. nov. is proposed. The type strain is TMPB967T (=KCTC 82621T=MCCC 1K05476T).
Induction, decay, and determinants of functional antibodies following vaccination with the RTS,S malaria vaccine in young children
Authors: Feng Gaoqian , Kurtovic Liriye , Agius Paul A. , Aitken Elizabeth , Sacarlal Jahit , Wines Bruce , Hogarth P. Mark , Rogerson Stephen , Fowkes Freya J. I. , Dobaño Carlota , Beeson James G. 
Published: May 2022

ABSTRACTBACKGROUNDRTS,S is the first malaria vaccine recommended for implementation among young children at risk. However, vaccine efficacy is modest and short-lived. Antibodies play the major role in vaccine-induced immunity, but knowledge on the induction, decay, and determinants of antibody function is limited, especially among children. Antibodies that promote opsonic phagocytosis and other cellular functions appear to be important contributors to RTS,S immunity.METHODSWe studied a phase IIb trial of RTS,S/AS02 conducted young children in malaria endemic regions of Mozambique. We evaluated the induction of antibodies targeting the circumsporozoite protein (CSP, vaccine antigen) that interact with Fcγ-receptors (FcRγs) and promote phagocytosis (neutrophils, monocytes, THP-1 cells), antibody-dependent respiratory burst (ADRB) by neutrophils, and natural killer (NK) cell activity, as well as the temporal kinetics of responses over 5 years of follow-up (ClinicalTrials.gov registry number NCT00197041).RESULTSRTS,S vaccination induced CSP-specific IgG with FcγRIIa and FcγRIII binding activity and promoted phagocytosis by neutrophils, THP-1 monocytes, and primary human monocytes, neutrophil ADRB activity, and NK cell activation. Responses were highly heterogenous among children, and the magnitude of neutrophil phagocytosis by antibodies was relatively modest, which may reflect modest vaccine efficacy. Induction of functional antibodies was lower among children with higher malaria exposure. Functional antibodies largely declined within a year post-vaccination, consistent with the decline in vaccine efficacy over that time, and decay rates varied for different antibody parameters. Biostatistical modelling suggested IgG1 and IgG3 contribute in promoting FcγR binding and phagocytosis, and IgG targeting the NANP-repeat and C-terminal regions CSP were similarly important for functional activities.CONCLUSIONSResults provide new insights to understand the modest and time-limited efficacy of RTS,S in children, and the induction of antibody functional activities. Improving the induction and maintenance of antibodies that promote phagocytosis and cellular functions, and combating the negative effect of malaria exposure on vaccine responses are potential strategies for improving RTS,S efficacy and longevity.
Behavior of cetaceans in the waters south of Pico Island (Azores, Portugal) - data obtained during whale-watching 2020
Authors: Zahn Peter , Kulike Lynn , Bloechl Armin 
Published: May 2022

AbstractLand-based and boat-based surveys were conducted to collect data during whale-watching excursions in July/August and October 2020. Occurrence and behavior of cetaceans south of Pico Island (Azores) were determined. 10 species were sighted. The most frequently sighted species were: Sperm whale (Physeter macrocephalus), Sei whale (Balaenoptera borealis), Atlantic spotted dolphin (Stenella frontalis), Common bottlenose dolphin (Tursiops truncatus), and Risso’s dolphin (Grampus griseus). 51 different behaviour patterns were recorded. The majority, 32 behavior, were observed from two or more species. 19 behavioral patterns were observed only once from a single species, Atlantic spotted dolphins showed nine and Sperm whale showed six. The most behavioral patterns were displayed by Stenella frontalis with 26 and Pseudorca crassidens with 22. Interspecific cooperative hunting and intraspecific food sharing were observed, for the latter there were no known observations in the Azores so far.
CRISPR-Mediated Generation and Characterization of a Gaa Homozygous c.1935C>A (p.D645E) Pompe Disease Knock-in Mouse Model Recapitulates Human Infantile Onset-Pompe Disease
Authors: Kan Shih-hsin , Huang Jeffrey Y. , Harb Jerry , Rha Allisandra , Dalton Nancy D. , Christensen Chloe , Chan Yunghang , Davis-Turak Jeremy , Neumann Jon , Wang Raymond Y. 
Published: May 2022

AbstractPompe disease (PD) is an autosomal recessive disorder caused by deficient lysosomal acid α-glucosidase (GAA), leading to reduced degradation and subsequent accumulation of intra-lysosomal glycogen in tissues, especially skeletal and oftentimes cardiac muscle. The c.1935C>A (p.Asp645Glu) variant is the most frequent GAA pathogenic mutation in people of Taiwanese and Southern Chinese ethnicity, causing infantile-onset PD (IOPD), which presents neonatally with severe hypertrophic cardiomyopathy, profound muscle hypotonia, and respiratory failure leading to premature death if untreated.To further investigate the pathogenic mechanism and facilitate development of therapies pertaining to this variant, we applied CRISPR-Cas9 homology-directed repair (HDR) using a novel dual sgRNA approach flanking the target site to generate a GaaEm1935C>A knock-in mouse model as well as a myoblast cell line carrying the Gaa c.1935C>A mutation. Herein we describe the molecular, biochemical, physiological, histological, and behavioral characterization of 3-month-old homozygous GaaEm1935C>A mice.Homozygous GaaEm1935C>A knock-in mice exhibited normal Gaa mRNA expression levels relative to wild-type mice, but GAA enzymatic activity was almost completely abolished, leading to a substantial increase in tissue glycogen storage, and significant concomitant impairment of autophagy. Echocardiography of 3-month-old knock-in mice revealed significant cardiac hypertrophy. The mice also demonstrated skeletal muscle weakness but, paradoxically, not early mortality. Longitudinal studies of this model, including assessment of its immune response to exogenously supplied GAA enzyme, are currently underway.In summary, the GaaEm1935C>A knock-in mouse model recapitulates the molecular, biochemical, histopathologic, and phenotypic aspects of human IOPD caused by the GAA c.1935C>A pathogenic variant. It is an ideal model to assess innovative therapies to treat IOPD, including personalized therapeutic strategies that correct pathogenic variants, restore GAA activity and produce functional phenotypes.
Finding rearrangements in nanopore DNA reads with last and dnarrange
Authors: Frith Martin C. , Mitsuhashi Satomi 
Published: May 2022

AbstractLong-read DNA sequencing techniques such as nanopore are especially useful for characterizing complex sequence rearrangements, which occur in some genetic diseases and also during evolution. Analyzing the sequence data to understand such rearrangements is not trivial, due to sequencing error, rearrangement intricacy, and abundance of repeated similar sequences in genomes.The last and dnarrange software packages can resolve complex relationships between DNA sequences, and characterize changes such as gene conversion, processed pseudogene insertion, and chromosome shattering. They can filter out numerous rearrangements shared by controls, e.g. healthy humans versus a patient, to focus on rearrangements unique to the patient. One useful ingredient is last-train, which learns the rates (probabilities) of deletions, insertions, and each kind of base match and mismatch. These probabilities are then used to find the most likely sequence relationships/alignments, which is especially useful for DNA with unusual rates, such as DNA from Plasmodium falciparum (malaria) with ∼ 80% a+t. This is also useful for less-studied species that lack reference genomes, so the DNA reads are compared to a different species’ genome. We also point out that a reference genome with ancestral alleles would be ideal.
Heavy–tailed neuronal connectivity arises from Hebbian self–organization
Authors: Lynn Christopher W. , Holmes Caroline M. , Palmer Stephanie E. 
Published: May 2022

AbstractIn networks of neurons, the connections are heavy–tailed, with a small number of neurons connected much more strongly than the vast majority of pairs.1–6 Yet it remains unclear whether, and how, such heavy–tailed connectivity emerges from simple underlying mechanisms. Here we propose a minimal model of synaptic self–organization: connections are pruned at random, and the synaptic strength rearranges under a mixture of Hebbian and random dynamics. Under these generic rules, networks evolve to produce scale–free distributions of connectivity strength, with a power–law exponent that depends only on the probability p of Hebbian (rather than random) growth. By extending our model to include correlations in neuronal activity, we find that clustering—another ubiquitous feature of neuronal networks6–9—also emerges naturally. We confirm these predictions in the connectomes of several animals, suggesting that heavy–tailed and clustered connectivity may arise from general principles of self–organization, rather than the biophysical particulars of individual neural systems.
Users' mental models of cross-device search under controlled and autonomous motivations
Authors: Dong Jing , Duan Ruoyang , Liang Shaobo 
Published: May 2022

PurposeExisting literature has elicited the external behaviors of cross-device search but not much is known about users' cognition of cross-device search. The study aims to explore how users perceive the cross-device search by combining with the mental models and how motivations affect the users' understanding of cross-device search.Design/methodology/approachThe research questions are addressed through semi-structured face-to-face interviews with 59 users. Prior to the interview, the user is asked to complete a cross-device search task designed with the simulation of controlled and autonomous motivations to gain a real experience. The concepts of mental models are coded according to the constructivist grounded theory method.FindingsThe study finds the users' mental models of cross-device search consist of four dimensions: Element, Quality, Function and Issue. The effect of motivation on the mental models is tested as significant in terms of the Quality and Function aspects. The controlled motivation affects the user's perception of how the device switch influence the search and the autonomous motivation influences the user's opinion of search system functions.Originality/valueThe contribution of this study is found to extend the existing knowledge of cross-device search and update the mental models of information search in the current multi-device environment. The findings inform the future study of cross-device search and practices of search system design.
Lie subalgebras of so(3,1) up to conjugacy
Authors: Ghanam Ryad , Thompson Gerard , Bandara Narayana 
Published: May 2022

PurposeThis study aims to find all subalgebras up to conjugacy in the real simple Lie algebra so(3,1).Design/methodology/approachThe authors use Lie Algebra techniques to find all inequivalent subalgebras of so(3,1) in all dimensions.FindingsThe authors find all subalgebras up to conjugacy in the real simple Lie algebra so(3,1).Originality/valueThis paper is an original research idea. It will be a main reference for many applications such as solving partial differential equations. If so(3,1) is part of the symmetry Lie algebra, then the subalgebras listed in this paper will be used to reduce the order of the partial differential equation (PDE) and produce non-equivalent solutions.
Local perspectives on the cultural significance of rejuvenated heritage shophouses in George Town, Penang
Authors: Phua Jing Sern , Tan Keith Kay Hin 
Published: May 2022

PurposeThis research presents a comparative perception study towards rejuvenated, community-driven shophouses in George Town, Penang.Design/methodology/approachIt captures the opinions of two distinct age groups by utilizing a mixed-methods approach encompassing quantitative (main) and qualitative (supporting) research to obtain a dynamic understanding of perceptions between younger and older residents in the city and how these impact the long-term sustainability of heritage conservation efforts. The distribution of questionnaires to residents of Penang was the primary data collection method, with the structure of the questionnaires supported by recent academic literature and past perceptional research studies about built heritage.FindingsThe four main findings from the study are therefore as follows: 1. Both age groups have a similar, positive perception towards the tangible, physical elements of rejuvenated shophouses. 2. Intangible, functional factors play an equal or stronger role in influencing people and their attitude towards public participation and overall “Sense of Place”. 3. The pessimistic “Sense of Place” responses from the “Older” group reflect an equally pessimistic attitude towards intangible, functional factors surrounding rejuvenated shophouses. 4. Youth-led changes to George Town's heritage shophouses are degrading the “sense of place” connecting the older generation to the city.Originality/valueThe study can serve as a guide for the development of more inclusive and socially sustainable conservation and adaptive re-use policies for safeguarding the heritage identity and value of shophouses for current and future generations to experience in a post-COVID world.
What is my calling? An exploratory mixed-methods approach to conceptualizing hospitality career calling
Authors: Lee Lindsey , Ponting Sandra Sun-Ah , Ghosh Ankita , Min Hyounae (Kelly) 
Published: May 2022

Purpose
This study aims to provide important insights in advancing the hospitality workforce by exploring the dimensions of calling. By identifying significant calling dimensions among hospitality employees, the study is guided by work as calling theory by also examining the mediating role of employees’ professional identity on intention to leave the industry.

Design/methodology/approach
This study used an exploratory mixed-methods approach. Study 1 included an online qualitative survey to explore the significant dimensions of calling among hospitality employees. Study 2 measured the significance of hospitality calling dimensions on intention to leave the industry, mediated by professional identity.

Findings
Study 1 identified transcendent summons, passion and purposeful life as significant dimensions of hospitality calling. Study 2 examined calling as a second-order construct with the aforementioned dimensions and proposed calling increases professional identity and decreases intention to leave the industry. However, professional identity did not significantly influence the intention to leave the industry.

Originality/value
This study brings value to the calling literature by exploring the calling dimensions unique to the hospitality workforce. Findings also highlight that subjective professional identity alone cannot lower employees’ intention to leave the industry. Organizational and industry support focusing on transcendent summons, passion and purposeful life are recommended.