bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of VSV‐ΔG‐spike production process with the Ambr15 system for a SARS‐COV‐2 vaccine
Authors: Rosen Osnat , Jayson Avital , Goldvaser Michael , Dor Eyal , Monash Arik , Levin Lilach , Cherry Lilach , Lupu Edith , Natan Niva , Girshengorn Meni , Epstein Eyal 
Published: March 2022

AbstractTo face the coronavirus disease 2019 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) virus, our institute has developed the rVSV‐ΔG‐spike vaccine, in which the glycoprotein of vesicular stomatitis virus (VSV) was replaced by the spike protein of SARS‐CoV‐2. Many process parameters can influence production yield. To maximize virus vaccine yield, each parameter should be tested independently and in combination with others. Here, we report the optimization of the production of the VSV‐ΔG‐spike vaccine in Vero cells using the Ambr15 system. This system facilitates high‐throughput screening of process parameters, as it contains 24 individually controlled, single‐use stirred‐tank minireactors. During optimization, critical parameters were tested. Those parameters included: cell densities; the multiplicity of infection; virus production temperature; medium addition and medium exchange; and supplementation of glucose in the virus production step. Virus production temperature, medium addition, and medium exchange were all found to significantly influence the yield. The optimized parameters were tested in the BioBLU 5p bioreactors production process and those that were found to contribute to the vaccine yield were integrated into the final process. The findings of this study demonstrate that an Ambr15 system is an effective tool for bioprocess optimization of vaccine production using macrocarriers and that the combination of production temperature, rate of medium addition, and medium exchange significantly improved virus yield.
A dynamic air–liquid interface system for in vitro mimicking of the nasal mucosa
Authors: Capuana Elisa , Fucarino Alberto , Burgio Stefano , Intili Giorgia , Manna Olga Maria , Pitruzzella Alessandro , Brucato Valerio , La Carrubba Vincenzo , Carfì Pavia Francesco 
Published: March 2022

AbstractThe development of an in vitro 3D model for the growth of the nasal mucosa cells can improve the therapy and the study of pathological states for subjects with chronic airway conditions. We have previously characterized a system consisting of a scaffold with an internal channel and a perfusion bioreactor with two independent flows provided by an external and an internal circuit, respectively. In this paper, this system was designed as a model of the nasal cavity, in which cells, grown on the inner surface of the scaffold channel, would be in contact at the same time with both culture medium, supplied by the external circuit, and air, provided with the internal flow. To ensure adequate nutrient supply to the cells in the scaffold channel, the radial diffusion of the culture medium through the porous matrix was evaluated first in qualitative and, then, in quantitative terms, demonstrating the capability of the system to control the value and direction of this flux. As a preliminary study, the culture of epithelial cells in the scaffold channel is also discussed in static, maintaining the air–liquid interface condition for up to 3 weeks. Despite minor abnormalities, such as a gap between cell layers and some detachments from the scaffold, the scaffold ensured cell survival and growth during the experimental time.
Secondary cancers after carbon‐ion radiotherapy and photon beam radiotherapy for uterine cervical cancer: A comparative study
Authors: Nitta Yuki , Murata Hiroto , Okonogi Noriyuki , Murata Kazutoshi , Wakatsuki Masaru , Karasawa Kumiko , Kato Shingo , Yamada Shigeru , Nakano Takashi , Tsuji Hiroshi 
Published: March 2022

AbstractBackgroundThere are limited studies on the risk of secondary cancers after carbon‐ion radiotherapy (CIRT). We assessed the incidence of secondary cancers in patients treated with CIRT for cervical cancer. We also evaluated the incidence of secondary cancers in patients who received standard photon radiotherapy (RT) throughout the same period.MethodsThis retrospective study included patients with cervical cancer who underwent curative RT at our hospital. All cancers discovered for the first time after RT were classified as secondary cancers. To compare the risk of secondary cancers among cervical cancer survivors to the general population, standardized incidence ratios (SIRs) were calculated.ResultsThe analysis included a total of 197 and 417 patients in the CIRT and photon RT groups, respectively. The total person‐years during the observation period were 1052.4 in the CIRT group and 2481.5 in the photon RT group. The SIR for all secondary cancers was 1.1 (95% confidence interval [CI], 0.6–2.1) in the CIRT group and 1.4 (95% CI, 1.0–2.1) in the photon RT group. The 10‐year cumulative incidence of all secondary cancers was 9.5% (95% CI, 4.0–21.5) in the CIRT group and 9.4% (95% CI, 6.2–14.1) in the photon RT group. The CIRT and photon RT groups were not significantly different in incidence (p = 0.268).ConclusionsThe incidence of secondary cancers after CIRT for cervical cancer was similar to that after photon RT. Validation of our findings after long‐term observation is warranted.
Self‐organisation in dialogical patterns of a patient with borderline personality disorder and their therapist: A case study from nonlinear dynamics perspective
Authors: Mellado Augusto , Guastello Stephen J. , Martínez Claudio , Tomicic Alemka , Krause Mariane 
Published: March 2022

AbstractObjectiveThe purpose of this study was to identify self‐organised dynamics in sessions with dialogical patterns (i.e. reflective interactions of self‐states associated with psychological change) within long‐term psychotherapy. One of the hallmarks of self‐organisation is the presence of an Inverse Power Law (IPL) in the frequency distribution of relevant events, which reflect better adaptation to internal and environmental changes, produced by psychotherapy.MethodsA case study of successful psychotherapy (44 weekly sessions) with a patient diagnosed with borderline personality disorder (BPD) and the therapist was conducted. The expressions of the subjectivity (i.e. voices) in the patient and therapist were identified through the Model of Analysis of Discursive Positioning in Psychotherapy (MAPP). State Space Grids (SSG) identified sessions that presented dialogical patterns acting as attractors (8 sessions) and those with non‐dialogical patterns acting as attractors (12 sessions). Finally, Orbital Decomposition (ORBDE) produced statistics of self‐organisation in both groups of sessions and the degree of fit to an IPL.ResultsBoth groups of sessions presented an acceptable fit to the IPL distributions (R2 = .853 and .938), and statistics placed them in different self‐organised regions.ConclusionsAlthough dialogical patterns are an emergent property of therapy, their structure is similar to sessions of other types, in that dynamic processes occur in them that can be empowered in patients to consolidate psychological change. The nonlinear and emergent character of these self‐organised processes and their clinical implications for activating the patients' psychological resources are discussed.
Chemoenzymatic Isolation and Characterization of High Purity Mammalian Melanin
Authors: Kalaj Brianna N. , Ni Qing Zhe , La Clair James J. , Deheyn Dimitri D. , Burkart Michael D. 
Published: March 2022

AbstractAlthough melanin is one of the most ubiquitous polymers in living systems, our understanding of its molecular structure, biosynthesis and biophysical properties has been limited to only a small number of organisms other than humans. This is in part due to the difficulty associated with isolating pure melanin. While purification methods exist, they typically involve harsh treatments with strong acid/base conditions combined with elevated temperatures that can lead to the polymer backbone degradation. To be successful, a viable isolation method must deliver a selective, yet complete degradation of non‐melanin biopolymers as well as remove small molecule metabolites that are not integrative to the melanin backbone. Here, we demonstrate the use of chemoenzymatic processing guided by fluorescent probes for the purification and isolation of native mammalian melanin without significant induction of chemical degradation. This multi‐step purification‐tracking methodology enables quantitative isolation of pure melanin from mammalian tissue for spectroscopic characterization.
Isolation of Tobacco Mosaic Virus‐Binding Peptides for Biotechnology Applications
Authors: Chan Soo Khim , Steinmetz Nicole F. 
Published: March 2022

AbstractTobacco mosaic virus (TMV) was the first virus to be discovered and it is now widely used as a tool for biological research and biotechnology applications. TMV particles can be decorated with functional molecules by genetic engineering or bioconjugation. However, this can destabilize the nanoparticles, and/or multiple rounds of modification may be necessary, reducing product yields and preventing the display of certain cargo molecules. To overcome these challenges, we used phage display technology and biopanning to isolate a TMV‐binding peptide (TBPT25) with strong binding properties (IC50=0.73 μM, KD=0.16 μM), allowing the display of model cargos via a single mixing step. The TMV‐binding peptide is specific for TMV but does not recognize free coat proteins and can therefore be used to decorate intact TMV or detect intact TMV particles in crude plant sap.
Functional Groups Assisted Tunable Dielectric Permittivity of Guest‐Free Zn‐Based Coordination Polymers for Gate Dielectrics
Authors: Kamal Saqib , Inamdar Arif I. , Chiou Kuan‐Ru , Sainbileg Batjargal , Usman Muhammad , Chen Jenq‐Wei , Luo Tzuoo‐Tsair , Hayashi Michitoshi , Hung Chen‐Hsiung , Liaw Wen‐Feng , Lu Kuang‐Lieh 
Published: March 2022

AbstractThe dielectric properties of coordination polymers has been a topic of recent interest, but the role of different functional groups on the dielectric properties of these polymers has not yet been fully addressed. Herein, the effects of electron‐donating (R=NH2) and electron‐withdrawing (R=NO2) groups on the dielectric behavior of such materials were investigated for two thermally stable and guest‐free Zn‐based coordination polymers, [Zn(L1)(L2)]n (1) and [Zn(L1)(L3)]n (2) [L1=2‐(2‐pyridyl) benzimidazole (Pbim), L2=5‐aminoisophthalate (Aip), and L3=5‐nitroisophthalate (Nip)]. The results of dielectric studies of 1 revealed that it possesses a high dielectric constant (κ=65.5 at 1 kHz), while compound 2 displayed an even higher dielectric constant (κ=110.3 at 1 kHz). The electron donating and withdrawing effects of the NH2 and NO2 substituents induce changes in the polarity of the polymers, which is due to the inductive effect from the aryl ring for both NO2 and NH2. Theoretical results from density functional theory (DFT) calculations, which also support the experimental findings, show that both compounds have a distinct electronic behavior with diverse wide bandgaps. The significance of the current work is to provide information about the structure‐dielectric property relationships. So, this study promises to pave the way for further research on the effects of different functional groups on coordination polymers on their dielectric properties.
Theoretical Studies on a Rotating Film Reactor for Hydrogen Production from Methane
Authors: Becker Tobias , Agar David W. 
Published: March 2022

AbstractA film of liquid metal can protect the reactor wall from carbon deposits during methane pyrolysis, discharge carbon from the reactor and prevent blocking. The film can be generated by rotation in a rotating film reactor. Parameters such as liquid volume flow, operating mode or the diameter of the reactor can have an influence on the reaction behavior. A design of experiments was used to investigate the rotating film reactor in more detail and to characterize the influence of various parameters.
The SQ HDM SLIT‐Tablet is safe and well tolerated in patients with House Dust Mite allergic rhinitis with or without asthma: A “real‐life” French study
Authors: Demoly Pascal , Leroyer Christophe , Serrano Elie , Le Maux Annelore , Magnier Gabrielle , Chartier Antoine 
Published: March 2022

AbstractBackgroundThe SQ House Dust Mite (HDM) SubLingual ImmunoTherapy (SLIT)‐Tablet (Acarizax) is the only allergen immunotherapy authorized by European regulatory authorities to treat HDM‐induced allergic asthma (AA) that is not well‐controlled by inhaled corticosteroids and associated with mild‐to‐severe HDM allergic rhinitis (AR). The aim of this study was to add evidence on the safety of the SQ HDM SLIT‐Tablet in patients with AR, alone or with AA, under real‐life conditions.MethodsThis was a French “real‐life”, multicenter, non‐comparative, longitudinal, prospective study. It included patients initiating the SQ HDM SLIT‐Tablet for either persistent moderate‐to‐severe HDM AR or AA not well‐controlled by inhaled corticosteroids and associated with mild‐to‐severe HDM AR. Adverse Events (AEs) were collected at the first intake and throughout the study. Logistic regression was used to compare safety according to asthma control before treatment initiation.ResultsBetween May 09, 2018 and May 29, 2019, 1526 patients were enrolled at 185 sites and 1483 were included in the safety population (SAF). Of them, 33.6% had suspected clinical manifestations of AA. Asthma was uncontrolled for 18.2% of the patients, partially controlled for 27.9% and well‐controlled for 53.8%. Overall, 31.9% of the SAF patients experienced at least one AE. The percentage of patients with AEs was 29.9% among patients with AR alone and 35.9% among those with AA (p = 0.0193). No significant difference was observed in the rate of AE or SAE depending on asthma control at inclusion (2.2% of SAEs reported for patients with uncontrolled asthma, 1.4% for partly controlled and 1.1% for well‐controlled).ConclusionsThe overall results indicate a good SQ HDM SLIT‐Tablet safety profile consistent with that reported in previous studies, regardless of asthma control.
Durvalumab and tremelimumab with definitive chemoradiotherapy for locally advanced esophageal squamous cell carcinoma
Authors: Park Sehhoon , Oh Dongryul , Choi Yoon‐La , Chi Sang Ah , Kim Kyunga , Ahn Myung‐Ju , Sun Jong‐Mu 
Published: March 2022

BackgroundThe current standard treatment for patients with inoperable, locally advanced esophageal squamous cell carcinoma (ESCC) is definitive concurrent chemoradiotherapy (CCRT).MethodsPatients with locally advanced ESCC received 2 cycles of 5‐fluorouracil, cisplatin, durvalumab, and tremelimumab every 3 weeks with concurrent radiation therapy (60.2 or 64.5 grays). After completing CCRT plus immunotherapy, patients received 2 cycles of consolidative durvalumab and tremelimumab followed by durvalumab monotherapy every 4 weeks for 2 years after enrollment. Their survival outcomes were compared with those from a propensity score‐matched historical control group that had received CCRT alone.ResultsIn total, 40 patients were enrolled and analyzed. The 24‐month progression‐free survival (PFS) and overall survival rates were 57.5% and 75%, respectively. Compared with the historical control group (n = 75), the study population had significantly longer PFS (hazard ratio [HR], 0.52; 95% confidence interval [CI], 0.28‐0.97; P = .040) and overall survival (HR, 0.49; 95% CI, 0.25‐0.98; P = .043). In the study population, patients who had PD‐L1–positive tumors (n = 28) had significantly longer PFS (HR, 0.20; 95% CI, 0.07‐0.54; P < .001) and overall survival (HR, 0.16; 95% CI, 0.05‐0.56; P = .001) compared with those who had PD‐L1–negative tumors (n = 11). However, there was no difference in survival outcomes according to PD‐L1 status in the historical control group, indicating a strong interaction between PD‐L1–positive status and survival outcomes in the treatment groups (PFS, P for interaction = .003; overall survival, P for interaction = .002).ConclusionsDurvalumab and tremelimumab with definitive CCRT had promising efficacy in patients with locally advanced ESCC. In addition, PD‐L1 expression had strong predictive value in the study population.
Darknet on OpenCL: A multiplatform tool for object detection and classification
Authors: Sowa Piotr , Izydorczyk Jacek 
Published: March 2022

AbstractThe goal of this article is to overview the challenges and problems on the way from the state‐of‐the‐art CUDA accelerated neural network code to multi‐GPU code. For this purpose, the authors describe the journey of porting that existing in GitHub, a fully featured CUDA‐accelerated Darknet engine, to OpenCL. This article presents the lessons learned and the techniques that were put in place for this porting. There are few other implementations on GitHub that leverage the OpenCL standard, and a few have tried to port Darknet as well. Darknet is a well‐known convolutional neural network (CNN) framework. The authors of this article investigated all aspects of porting and achieved a fully featured Darknet engine on OpenCL. The effort was focused not only on classification using YOLO1, YOLO2, YOLO3, and YOLO4 CNN models. Other aspects were also covered, such as training neural networks and benchmarks to identify weak points in the implementation. Compared with the standard CPU version, the GPU computing code substantially improves the Darknet computing time by using underutilized hardware in existing systems. If the system is OpenCL‐based, it is practically hardware‐independent. The authors also improved the CUDA version as Darknet‐vNext.
A Review on Carbon Quantum Dot Based Semiconductor Photocatalysts for the Abatement of Refractory Pollutants
Authors: M. Athulya , John Bony K. , Chacko Anu Rose , Mohan Chitra , Mathew Beena 
Published: March 2022

AbstractPhotocatalysis is a green approach frequently utilised to eliminate a variety of environmentally hazardous refractory pollutants. Accordingly, the modification of semiconductor photocatalysts with Carbon Quantum Dots (CQDs) is of great importance for the treatment of such pollutants due to their attractive physical and chemical properties. CQDs are a perfect candidate to handle photocatalysts of high‐performance since they operate as co‐catalysts and as visible light harvesters. The higher separation rate of electron‐hole pairs in the photocatalytic system is attributable to better photodegradation efficiency. This review classifies CQD based photocatalysts as pure, doped and composite materials and discusses the specific advantages of CQDs in visible light‐driven photocatalysis. In this work, the versatile roles of CQDs in CQD‐based photocatalytic systems are thoroughly discussed and summarised.
The influence of corporate social responsibilities on sustainable financial performance: Mediating role of shared vision capabilities and moderating role of entrepreneurship
Authors: Luo Wenbo , Zhang Chuan , Li Minxin 
Published: March 2022

AbstractThis study examined the roles of economic, social, and environmental proactive corporate social responsibility (CSR) on sustainable financial performance among small and medium‐sized enterprises (SMEs) in China. The study also explored the moderating role of entrepreneurship and mediating role of shared vision capabilities in the relationships between economic, social, and environmental proactive CSR and sustainable financial performance for SMEs in China. This quantitative study used questionnaires to collect data from respondents. The PLS‐SEM method was used to analyse a sample of 490 Chinese SMEs in the manufacturing sector. The results indicated that economic, social, and environmental proactive CSR were positively associated with sustainable financial performance. The results also showed that shared vision capabilities positively mediated the relationships between economic, social, and environmental proactive CSR and sustainable financial performance. Entrepreneurship significantly moderated the relationships between economic and social proactive CSR and sustainable financial performance. The findings of this study are suitable for policymakers when developing policies related to CSR, entrepreneurship, and sustainable financial performance.
Advancements of Polyvinylpyrrolidone‐Based Polymer Electrolyte Membranes for Electrochemical Energy Conversion and Storage Devices
Authors: Li Wen , Wang Haining , Zhang Jin , Xiang Yan , Lu Shanfu 
Published: March 2022

AbstractPolymer electrolyte membranes (PEMs) play vital roles in electrochemical energy conversion and storage devices, such as polymer electrolyte membrane fuel cell (PEMFC), redox flow battery, and water electrolysis. As the crucial component of these devices, PEMs need to possess high ion conductivity and electronic insulation, remarkable mechanical and chemical stability, and outstanding isolation function for the materials on both sides of the cathode and anode. Polyvinylpyrrolidone has received widespread attention in the research of PEMs owing to its tertiary amine basic groups and exceptional hydrophilic properties. This review focuses on the application status of polyvinylpyrrolidone‐based PEMs in PEMFC, vanadium redox flow battery, and alkaline water electrolysis, and describes in detail the key scientific problems in these fields, providing constructive suggestions and guidance for the application of polyvinylpyrrolidone‐based PEMs in electrochemical energy conversion and storage devices.
A weighted range‐free localization algorithm for irregular multihop networks
Authors: Yan Xiaoyong , Zhou Jian , Huang Haiping , Wu Chenhuang , Sun Lijuan , Song Aiguo 
Published: March 2022

SummaryRange‐free localization is a widely concerned technique that supports many essential network applications like coverage and clustering. Most existing localization technologies assume that nodes are uniformly distributed in a regular network with no obstacles and that propagation paths between pairwise nodes are close to straight lines. However, in real‐world scenarios, network topology is usually irregular, resulting in estimated distances that differ significantly from the corresponding physical distances and poor anchor deployment. These will lead to erroneous distance measurement and inaccurate localization results. This paper proposes a new algorithm, called the weighted range‐free localization algorithm (WRL for short), to improve the localization performance in irregular networks. Specifically, we first present a feasible scheme that can fulfill the information exchange and relative distance estimation for each node simultaneously. We then employ an optimal weight function to suppress the effect of cumulative errors and introduce the weighted bounding‐box algorithm to mitigate outlier location estimation induced by probably collinear anchor nodes. Simulation results show that, compared to state‐of‐the‐art methods, WRL improves localization accuracy while incurring lower computation and communication overheads in various irregular network topologies.