bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using short tandem repeat profiling to validate cell lines in biobanks
Authors: Kosobokova E. N. , Malchenkova A. A. , Kalinina N. A. , Kosorukov V. S. 
Published: December 2022

Aim. To approve the COrDIS kit (Gordiz, Russia) for the authenticity of cell lines from the Bioresource Collection of the N.N. Blokhin National Medical Research Center of Oncology by the short tandem repeat (STR) profiling.Material and methods. The chosen method proved to be a reliable and reproducible option. With this approach, a number of polymorphic STR loci are amplified using commercially available primer sets. Polymerase chain reaction (PCR) products are analyzed simultaneously with size standards using automated fluorescent detection methods. The results are presented as a simple number code corresponding to the lengths of the PCR products amplified at each locus. By applying this method to cell lines, the laboratory can both authenticate commercial cell lines and build a database of their lines. In the work, we used the COrDIS EXPERT 26 kit (Gordiz, Russia), validated for molecular genetic identification of personality based on multiplex PCR analysis of 26 highly polymorphic loci of human genomic deoxyribonucleic acid. PCR results were analyzed by capillary electrophoresis using an automatic genetic analyzer with laser-induced fluorescence detection (Applied Biosystems 3500xL).Results. When testing the method, profiling of 37 cell lines was carried out, of which 18 were announced in international databases and 19 were unique, obtained at the N. N. Blokhin National Medical Research Center of Oncology, as well as a cell line mixture in order to determine the limits of contamination detection. The obtained results showed the correspondence of commercial cell lines with the data in international databases. Within the framework of this work, profiles of unique lines were obtained and the foundation of own genetic database was laid. Studies to identify the limit of contamination detection by another line have shown that even 4% of the contaminant culture in the total pool can be used to identify its individual alleles.Conclusion. The results obtained indicate the possibility of using the method to identify samples of the collection and detect intraspecific contamination.
Population biobank as a basis for determining spatial variation of clinically relevant pharmacogenetic biomarkers of cardiovascular diseases
Authors: Pylev V. Yu. , Agdzhoyan A. T. , Gorin I. O. , Petrushenko V. S. , Pocheshkhova E. A. , Mirzaev K. B. , Balanovskaya E. V. 
Published: December 2022

The introduction of pharmacogenetic tests among the Russian population faces a fundamental limitation — pronounced genetic differences between populations. The genetic geography of pharmacogenetic markers of deoxyribonucleic acid (DNA) helps to remove these limitations.Aim. To reveal the spatial variation of the gene pools of the indigenous European Russian population in terms of DNA markers that are significant for the pharmacotherapy of cardiovascular diseases (CVDs) using the population biobank collections.Material and methods. A total of 3170 samples from 61 populations of the Biobank of Northern Eurasia, which represents the gene pools of the indigenous Eastern Europe population, were studied using two pharmacogenetic DNA marker arrays as follows: 60 most significant markers and 24 markers associated with CVDs. Using the multivariate statistics and genetic geography, a comparison of gene pool variation was made.Results. A cartographic atlas has been created that includes maps of the distribution among the Eastern Europe population of 24 pharmacogenetic CVD markers. These cartographic models allow various specialists to analyze patterns in the distribution of pharmacogenetic markers. General patterns are supplemented by regional studies in the North Caucasus, the Cisurals and the Russian Plain, which identify population groups with similar pharmacogenetic status. For each region, a comparison of gene pool variation for two arrays of above-mentioned DNA markers was made.Conclusion. The created atlas is the basis for the development of pharmacogenetic studies conducted by genetic geography methods using a single panel of markers and representative samples provided by population biobanks. The reliability of the results is ensured by a detailed genealogical and population annotation of each biobank sample and representative samples from the populations.
How News Reports and ESG Publication Financially Affect Firms
Authors: Anang Eric , Васиев Максим , Wang Qian 
Published: December 2022

ESG has become a growing and integral part of company activities. Not only is it something investors now take into consideration when choosing stocks, but the question of a firm’s Environmental, Social and Governance awareness and actions has integrated itself into something as critical as some banks’ decisions to provide loans to a firm. While ESG used to bejust a “nice-to-have,” there is no denying that it is now a must-have for any company that wants to be a global market leader.
Many prior studies have focused on ESG ratings, the types of ESG information disclosed in annual reports and the effect of financial news on firms. The aim of this research is to take a deeper look at the effect of ESG on firms and find out whether different news feed and news publications concerning a company’s ESG activities and circumstances affect its value? Our research shows that there is a weakly significant effect of negative ESG-related news on firms in the window (–1, +1) and no significant reaction to positive news. This means that investors do not statistically significantly react to positive ESG news about firms. We examined 65 publicly traded companies from 7 different markets worldwide over the course of 13 years (from 2009 to 2021). We collected a total of 458 separate news articles from the S&P Global Market Intelligence platform –and classified them into positive and negative depending on the news. We ran OLS regressions of the data points together with financial control variables on a company’s CAR in a 5-, 3- and 2-day interval to check for the effect. No significant effect was reported.
Openreuma Consensus on the role of nursing in the care of patients with rheumatoid arthritis and diffuse interstitial lung disease
Authors: Cano García Laura , García de Yébenes María Jesús , Mena Vázquez Natalia , Martín Martín José Ma , Domínguez Quesada Carmen , García-Díaz Silvia , Rodríguez Vargas Ana Isabel , Jiménez Núñez Francisco , Espíldora Hernández Francisco , León Mateos Leticia , Vázquez Lojo Ana , Marcos Pérez Elena , Castiblanco Laura , Carmona Loreto 
Published: December 2022

Objective. To develop practical recommendations, based on the best available evidence and experience, on the nursing management of patients with rheumatoid arthritis (RA) and interstitial lung disease (ILD).
Methods. The usual consensus methodology was used, with a nominal group, systematic reviews (SRs), and Delphi survey. The expert panel, consisting of rheumatology nurses, rheumatologists, a psychologist, a physiotherapist, and a patient, defined the scope, the users, the topics on which to explore the evidence and on which to issue recommendations.
Results. Three PICO questions evaluated the efficacy and safety of pulmonary rehabilitation and non-pharmacological measures for the treatment of chronic cough and gastroesophageal reflux by means of SR of the literature. With the results of the reviews, 15 recommendations were established for which the degree of agreement was obtained with a Delphi survey. Three recommendations were rejected in the second round. The 12 recommendations were in patient assessment (n=4); patient education (n=4); and risk management (n=4). Only one recommendation was based on available evidence, while the remaining were based on expert opinion. The degree of agreement ranged from 77% to 100%.
Conclusion. This document presents a series of recommendations with the aim of improving the prognosis and quality of life of patients with RA-ILD. Nursing knowledge and implementation of these recommendations can improve the follow-up and prognosis of patients with RA who present with ILD.
Role of arginase 1 immunohistochemical marker in differentiating hepatocellular carcinoma from other primary and secondary carcinomas of the liver, a tissue microarray study
Authors: Yussif Shaimaa M , Elzeftawy Dina H , Elshawaf Ibrahim M , Elkashef Wagdi F 
Published: December 2022

: Hepatocellular carcinoma (HCC) is the most prevalent type of primary cancer of the liver in adults represent about 80%-90% of all liver cancers. It is essential to differentiate primary HCC and intrahepatic cholangiocarcinoma and metastatic carcinoma. Arginase-1 was considered as the most sensitive and specific marker of benign and malignant hepatocyte. This study aimed to detect the diagnostic role of immunohistochemical expression of arginase-1 in differentiating HCC From cholangiocarcinoma and metastatic carcinomas of the liver in comparison with hepatocyte paraffin antigen -1 (HepPar-1) and Glypican 3. : This is a retrospective study was performed on 117 cases, 77 cases were diagnosed as HCC, 13 cases as cholangiocarcinoma and 27 cases as metastatic carcinomas in the liver. Cases obtained from surgical pathology laboratory at Gastroenterology Center, Mansoura University, Egypt during the period from 2014 to 2017. All the studied cases were immunostained with Arginase 1, Heppar 1and Glypican 3.: Arginase 1 was expressed in all 77 HCC cases with sensitivity (100%), while Arginase 1 was expressed only in 1 cholangiocarcinoma case and negative in other metastatic carcinomas with specificity(97.5%), the overall accuracy was (99.1%). On the other side, Glypican 3 was expressed in 36 out of 77 HCC cases with sensitivity (46.8%), while Glypican 3 was expressed in 3 out of 40 cholangiocarcinoma and other metastatic carcinomas with specificity (92.3%), overall accuracy (62.4%). Heppar 1 was expressed in 69 out of 77 HCC cases with sensitivity (89.6%), while Heppar 1 was negative in all cholangiocarcinoma cases and other metastatic carcinomas with specificity (100%). The overall accuracy was 93.2%.: Arginase 1 is the most sensitive and accurate marker in differentiating HCC from non HCC cases in liver, while heppar is most specific and second accurate marker in differentiating HCC from cholangiocarcinoma and metastatic carcinomas in the liver. Arginase-1 and HepPar-1, are the best markers regarding sensitivity and specificity for small liver biopsies.
The Effects of Body Percussion Exercise on Balance in Older Adults
Authors: Siminghalam Mona , Alibakhshi Hossein , Valikhani Negin , Gholami Maryam , Mirshoja Mina Sadat 
Published: December 2022

Introduction: One of the most complex problems of elders is the balance problem and the increased risk of falling. Body percussion is an effective treatment for various diseases with cognitive, physical, and psychological deficits and improves the memory, motor, and coordination skills, as well as the social interaction of patients with neurological diseases. One of the problems in the elderly is the weakness in balance skills and falling. This study aimed to investigate the effect of body percussion exercises (BPE) on the balance of the elderly.
Materials and Methods: This study was carried out as pretest-posttest research with two experimental and control groups. A 12-session therapeutic program was implemented 3 days a week each for 45-60 minutes. The subjects were evaluated using the Berg balance scale, functional reach, and timed up and go (TUG) tests. Data were analyzed by paired and independent t tests.
Results: The results of the treatment group showed a significant effect of BPE on the balance (P<0.005). A comparison of post-treatment results showed that the Berg balance scale (P=0.00), and timed up and go (P=0.023) were significantly different. The functional reach test (P=0.174) was not significantly different.
Conclusion: This study showed that BPE had a significant effect on the elderly`s balance.
Evaluation of Interventions to Improve Ventilation in Households to Reduce Risk for Transmission of Severe Acute Respiratory Syndrome Coronavirus 2
Authors: Ha Wilson , Stiefel Mitchell , Gries Jeremy , Cadnum Jennifer , Torres-Teran Maria , Wilson Brigid , Donskey Curtis 
Published: December 2022

Background: Inadequate ventilation may contribute to the high risk for household transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: We evaluated the effectiveness of several interventions recommended to improve ventilation in households. In 7 residential homes, carbon dioxide monitoring was conducted to assess ventilation in occupied open areas such as family rooms and in bedrooms and/or offices. Carbon dioxide levels above 800 parts per million (ppm) were considered an indicator of suboptimal ventilation for the number of people present. In 1 of the 7 homes, various interventions to improve ventilation or to filter air were assessed in a kitchen area by measuring clearance of aerosol particles produced using an aerosol-based spray system and carbon dioxide generated by cooking with a gas stove. Results: Carbon dioxide levels rose above 800 ppm in bedrooms and offices with 2 occupants when windows and doors were closed and in open areas during gatherings of 5 to 10 people; carbon dioxide levels decreased when windows or doors were opened. Clearance of carbon dioxide and aerosol particles significantly increased with interventions including running fans, operating portable air cleaners, and opening windows, particularly when there was a noticeable breeze or when a window fan was used to blow contaminated air outside. Conclusion: In households, several measures to improve ventilation or air filtration were effective in reducing carbon dioxide accumulation or enhancing clearance of carbon dioxide and aerosol particles. Studies are needed to determine if interventions to improve ventilation can reduce the risk for airborne transmission of SARS-CoV-2 in households.
Effects of Segond fracture and associated injuries on knee stability
Authors: ruan siyuan , liu desen , gu liuwei , wang yuqi , huang xincheng , cao hong 
Published: December 2022

Abstract
Objective: To investigate the effects of Segond fracture and associated injuries on knee stability.
Methods: Retrospective analysis of 35 cases of Segond fracture, allpatients had anterior cruciate ligament rupture (100%), including 6 patients (17.1%) with avulsion fracture of tibia intercondylar eminence. There were 8 cases (22.9%) with medial collateral ligament injury. There were 5 cases (14.3%) with lateral collateral ligament injury. There were 20 cases (57.1%) with combined meniscus injury, 5cases (14.3%) with both medial and lateral meniscus injury, 3 cases (8.6%) with only medial meniscus injury, and 12cases (34.3%) with only lateral meniscus injury. All 35 patients underwent surgical treatment, including 35 cases of ligament reconstruction, 6 cases of intercondylar eminence fracture fixation, and 20 cases of meniscus repair. To compare the function and stability of knee joint before operation and 2 years after operation.
Results: The Lysholm score for knee joint function on admission was (43.9±5.3) and the IKDC score was (36.1±3.9). All 35 patients were followed up for 24-36 months. X-rays showed that the Segond fractures healed 3 months after surgery. The anterior drawer test, Lachman test, McMurray test, and Lateral stress test were all negative at the last follow-up. 5 patients with lateral meniscus injury had 1 grade pivot shift at the last follow-up. The Lysholm score was (91.7±3.6), and the difference was statistically significant as compared with that before surgery (P<0.05). The IKDC score was (89.0±1.7), and the difference was statistically significant as compared with that before surgery (P<0.05).
Conclusion: The indicative effect of Segond fracture on timely detection of combined injuries in the knee joint cannot be ignored. In addition to repairing the intra-articular injuries, more attention should be paid to the anterolateral structural stability of the knee joint. For Segond fracture patients with higher pivot shift grade may have residual axial migration after surgery, so ALL reconstruction surgery may be considered.
Exceptional minor rainy season inducing flooding of the UNESCO World Heritage site of Grand-Bassam (Côte d'Ivoire)
Authors: TOUALY ELISEE , S MARCEL PhD , Zokagoa Jean Marie 
Published: December 2022

Abstract

This work focuses on the time and space variability of the Comoé river’s flow in relation with the flooding in 2019 of the UNESCO World Heritage city of Grand Bassam (GB) in Côte d’Ivoire (CI). The rainfall, sea level, ocean swell and the Comoé river flow data have been used to conduct this work. This study showed that the Comoé river flow is higher in September-October. The maximum flow depends of the annual precipitation accumulated from the North to the South. During the rainy period, the flow at GB increases till to reach an absolute maximum during the minor rainy season in October. The event of October 2019 is an exceptional event due to strong minor rainy season compared to the others years. Besides this year, the minor rainy season was stronger than the major one. To solve the problem of flooding, opening the channel to rebuild the estuary linking the Comoé river to the sea appears as a solution. But precaution should be taken because of salinification and, during September-October strong swells and highest sea level are observed along the coast of GB. Besides, these strong swells are an opportunity for removing sediments from the mouth of the estuary one month before the critical month of October. Meanwhile using the water of the Comoé river for irrigation is an alternative solution. Observing the annual precipitation of a given year until the end of September could provide some information about the occurrence of flooding this year.
Causal analysis between rice growth and cadmium accumulation and transfer under arbuscular mycorrhizal inoculation
Authors: Zhao Ting , Wang Li , Yang Jixian , Ma Fang 
Published: December 2022

Abstract
Cadmium contamination of rice is a serious hazard to human health in recent years. To investigate the effects of AMF on cadmium translocation of rice, a pot experiment was adopted to compare and analyze the effects of AMF on the growth and Cd content of rice under seven soil Cd levels. The results showed that (1) AMF promoted the growth of rice especially the growth of root system. The number of large individuals of host plants increased and their plasticity was enhanced after AMF inoculation. (2) The Cd concentration in rice roots of AMF treatment group were significantly higher than those of blank control group, indicating that the AMF fixed more Cd in rice roots. It is the opposite in rice seeds and it indicated that the host plant actively reduce the Cd accumulation from the seed rather than dilution effect under AMF regulation. (3) The path coefficient of each variable were different in different treatment. It indicates that the direct effect of cadmium content in soil on the accumulation of cadmium content in seeds was reduced by AMF inoculation. In conclusion, the effect of mycorrhizal on cadmium transport in plants is complex. This is because the accumulation of Cd is always accompanied by the simultaneous growth dilution and compensatory accumulation caused by mycorrhizal effect, which is difficult to be quantitatively analyzed. The AMF inoculation is helpful to solve the safety of rice production under the condition of medium and light cadmium pollution
A physics based reservoir model for analyzing large amount of reservoir production data
Authors: Hiorth Aksel , Jettestuen Espen , Osmundsen Petter 
Published: December 2022

Abstract

50 years of fluid production from the Norwegian Continental Shelf (NCS) are available in the public domain. The data sets are not complete if one wants to use grid-based models, required by commercial reservoir simulators to history match the data. To make use of the data, it is necessary to return to simpler and more generic reservoir models. Many of the simpler models, such as decline curve models or pure data driven e.g., machine learning models are often not based on theory, which makes it hard to trust them outside the parameter range they have been tested and it is more complicated to extract generic knowledge. We propose a novel, simple reservoir model, with theoretical underpinnings from continuous flow models to analyse field production data from 102 fields from the NCS. The novelty in this work comes from our theoretical model which contains two parameters with a clear physical interpretation. Despite its simplicity, the model has a good match for production data from almost all the fields. Total production for most fields is matched within 5%. We demonstrate that one use of our model could be to evaluate the effect on hydrocarbon production if more or less wells than planned are drilled on a specific field. We present correlations which makes it possible to predict hydrocarbon production with limited data available.
Production of sterile trout (Triploids) by chromosome set manipulation using thermal shock treatment in rainbow trout (Oncorhynchus mykiss) from Kashmir Himalaya
Authors: Bazaz Asim Iqbal , Shah Tasaduq H. , Bhat Farooz A. , Ahmad Irfan , Kumar Ravindra , Kushwaha Basdeo , Abubakr Adnan , Bhat Bilal A. , Malik Rizwana , ul-Arab Nafhat 
Published: December 2022

Abstract
Rainbow trout is one of the promising cultivable fish species and has wide potential for its expansion. Being a low volume, high value commodity the trout has strong potential for both domestic consumption and international export. It is a cold water fish belonging to the family Salmonidae and requires an optimum temperature range of 10–15℃ for best growth. The current study to produce sterile trout (triploids) using thermal shock treatment in (O.mykiss) was carried out at the government trout farm Laribal, Srinagar. Heat shock at 26oC and 28oC for 10 min. duration were applied 15 min. and 20 min. after fertilization using a water bath. No treatment (heat shock) was given to Control. The highest fertilization rate (90.393 ± 0.37%) was observed in group T1, heat shocked at 26oC, after 15 minutes of fertilization (TAF) for 10 minutes. The highest hatching rate (81.843 ± 0.88%) among the treatment groups was observed in group T2, heat shocked at 26oC for 10 minutes after 20 minutes of fertilization. The highest yolk sac absorption rates for treatment groups was observed in T2 (83.94 ± 0.52%) heat shocked at 26oC, after 20 minutes of fertilization (TAF) for 10 minutes. T4 group showed the highest triploidy rate of 76.66 ± 3.33% heat shocked at 28oC, after 20 minutes of fertilization (TAF) for 10 minutes. However, no triploids were observed in control group. It is observed that temperature intensity showed significant negative correlation with fertilization rate and hatching rate (r= -0.168, p < 0.01; r= -0.492, p < 0.01) respectively. However, fertilization rates displayed significant negative (r= -0.378, p < 0.01) and hatching rates showed significant positive correlation with heat shock time after fertilization (r = 0.624, p < 0.01) respectively. Yolk sac absorption rate and triploidy rates showed significant positive correlation with heat shock time after fertilization (r = 0.778, p < 0.01; r = 0.382, p < 0.01) respectively. However, temperature intensity formed significant negative correlation with yolk sac absorption rates (r= -0.556, p < 0.01) while as significant positive correlation was observed between temperature intensity and triploidy rates (r = 0.891, p < 0.01).Control group showed higher survival rates at fertilization (93.16 ± 0.41%), hatching (84.28 ± 0.46) and at complete yolk sac absorption (86.27 ± 0.55%) compared to heat shocked groups.