bluuebunny/crossref_metadata_2025_split huggingface dataset:
Commercial ChIP-Seq library preparation kits performed differently for different classes of protein targets
Authors: Simper MS , Coletta L Della , Gaddis S , Lin K , Mikulec CD , Takata Y , Tomida MW , Zhang D , Tang DG , Estecio MR , Shen J , Lu Y 
Published: April 2022

ABSTRACTBackgroundChIP-Seq is a powerful method commonly used to study global protein-DNA interactions including both transcription factors and histone modifications. We have found that the choice of ChIP-Seq library preparation protocol plays an important role in overall ChIP-Seq data quality. However, very few studies have compared ChIP-Seq libraries prepared by different protocols using multiple targets and a broad range of input DNA levels.ResultsIn this study, we evaluated the performance of four ChIP-Seq library preparation protocols [NEB NEBNext Ultra II, Roche KAPA HyperPrep, Diagenode MicroPlex, and Bioo (now PerkinElmer) NEXTflex] on three target proteins, chosen to represent the three typical signal enrichment patterns in ChIP-Seq experiments: sharp peaks (H3K4me3), broad domains (H3K27me3) and punctate peaks with a protein binding motif (CTCF). We also tested a broad range of different input DNA levels from 0.10 to 10 ng for H3K4me3 and H3K27me3 experiments.ConclusionsOur results suggest that the NEB protocol may be better for preparing H3K4me3 (and potentially other histone modifications with sharp peak enrichment) libraries; the Bioo protocol may be better for preparing H3K27me3 (and potentially other histone modifications with broad domain enrichment) libraries, and the Diagenode protocol may be better for preparing CTCF (and potentially other transcription factors with well-defined binding motifs) libraries. For ChIP-Seq experiments using novel targets without a known signal enrichment pattern, the NEB protocol might be the best choice as it performed well for each of the three targets we tested across a wide array of input DNA levels.
Release of VAMP5-positive extracellular vesicles by retinal Müller gliain vivo
Authors: Demais Valerie , Pohl Anne , Wunderlich Kirsten A. , Pfaller Anna M. , Kaplan Lew , Barthélémy Amelie , Dittrich Robin , Puig Berta , Giebel Bernd , Hauck Stefanie M. , Pfrieger Frank W. , Grosche Antje 
Published: April 2022

AbstractCell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Müller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal Müller cells in adult micein vivo. We identify VAMP5 as a Müller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified Müller cells and neuronsin vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.
Exploring how social capital and self-esteem shape career success among women in a patriarchal African society: the case of Nigeria
Authors: Imhanrenialena Benedict Ogbemudia , Cross Ogohi Daniel , Ebhotemhen Wilson , Chukwu Benjamin Ibe , Oforkansi Ejike Sebastian 
Published: April 2022

Purpose The purpose of this research is to investigate how bridging and bonding social capital relate to career success among career women in a patriarchal African society. Further, the intervening role of self-esteem in the association between social capital and career success was examined.Design/methodology/approach Structured questionnaire was used to collect data from 488 Nigerian career women in management cadres in both private and public sectors. Partial least squares structural equation modeling was applied in testing the proposed hypotheses.Findings The outcomes show that bridging social capital has a significant positive relationship with subjective and objective career success. Conversely, bonding social capital has no significant positive relationship with subjective and objective career success. Further analyses show that self-esteem only partially mediates the association between bridging social capital and career success while an insignificant intervening effect of self-esteem on the association between bonding social capital and career success was found.Practical implications The findings suggest the need for organisations to stimulate a friendly work environment that has a zero-tolerance culture for workplace discrimination against women. This will enable the women to relate with people in the workplace irrespective of gender or cadre to generate more bridging social capital to achieve greater career success.Originality/value The study extends social capital and career success research to career women in a patriarchal African context as a response to the call for context-specific career research in non-western countries particularly Africa. Second, the study provides empirical evidence that African career woman with bridging social capital can achieve career success irrespective of their self-esteem level amid patriarchal discrimination.
Difficulties experienced by managers in the coordination of teams working from home: an exploratory study considering the COVID-19 pandemic
Authors: Rodrigues Edilson Alves , Rampasso Izabela Simon , Pavan Serafim Milena , Filho Walter Leal , Anholon Rosley 
Published: April 2022

PurposeThis research aims to analyse difficulties experienced by Brazilian managers in coordinating teams working from home during the coronavirus disease (2019) COVID-19 pandemic.Design/methodology/approachThe methodological strategy used was a survey with 39 managers who led teams working from home during the COVID-19 pandemic. Seven difficulties indicated by current literature were analysed using a fuzzy scale. First, a hierarchical cluster analysis (HCA) approach was used to group managers according to managers' similarities and capacity to infer the difficulties. Responses of each group identified were weighted considering the capacity to assess the theme. In the sequence, data were analysed via frequencies and the fuzzy technique for order of preference by similarity to ideal solution (TOPSIS) approach and difficulties were ordered.FindingsComparatively, the main difficulties evidenced are (1) to reconcile personal and professional life tasks in the same place; (2) to motivate collaborators in a period when social isolation affect employee's mental health and (3) to keep team members integrated and working within the activities scope in a virtual environment.Originality/valueThe findings present in this paper contribute to theory and practice. For theory, this article contributes to the knowledge on WFH and leadership, evidencing in a comparatively way the difficulties that are experienced by managers during the COVID-19 pandemic. Researchers in future studies can better analyse these difficulties. For practice, managers who conduct managers' teams remotely can use the information to analyse teams' practices and improve performance critically. This was an atypical moment of humanity, and different aspects need to be considered by managers compared with previous periods.
A deeper understanding of switching intention and the perceptions of non-subscribers
Authors: Tsai Ling Long 
Published: April 2022

PurposeMost previous studies on new technologies and services have concentrated on their acceptance, seldom exploring in depth why users may choose not to accept technology or service and remain “non-users.” This study aims to understand free platform users' intention to switch to paid subscription platforms.Design/methodology/approachThis study utilized push-pull-mooring (PPM) theory to investigate free OTT platform viewers' switching intentions toward paid OTT platforms. A research model was established and examined via a two-stage partial least square (PLS) method. A total of 446 free users were collected from Facebook and Line for data analysis.FindingsResults show that perceived intrusiveness is the push factor and alternative attractiveness is the pull factor and that both have a positive impact on the switching intention of non-subscribers. Habit represents the mooring factor and negative affects switching intention. Perceived convenience and perceived enjoyment are shown to be two significant habitual antecedents. Furthermore, habit is revealed to moderate the effect of users' perceived advertisement intrusion and alternative attractiveness on switching intention to strengthen positive impact when the habit is strong.Originality/valueThis study is one of the pioneering studies to consider free-to-paid switching behavior on media services using PPM's structural equation model. Contrary to previous research, the study found that, in the context of the free-to-paid transition, highly accustomed users' perception of pull factors and push factors were strengthened, thus generating the tendency to switch platforms.
Ongoing adaptive reuse: patterns of heritage resilience before and after COVID-19
Authors: Fava Federica 
Published: April 2022

PurposeThe paper aims to assess the impact and responses to coronavirus disease 2019 in six European heritage labs (Horizon 2020 Framework Programme) selected for their adaptive heritage re-use practices based on participation, self-organisation and self-management. As they are naturally oriented towards building resilient urban systems, the hypothesis is that the co-production of cultural values and places promoted by these projects could create the conditions for equitable perspectives of resilience in the normality of contemporary urban life.Design/methodology/approachThe paper draws on data collected through a survey of six European Living Labs between January and May 2021. The survey results are framed by a literature review that defines adaptive reuse in terms of resilience. The five resilience characteristics described by Judith Rodin (awareness, diversification, integration, self-regulation and adaptability) are used to navigate the literature and organise the survey results.FindingsCombining survey results and insights from the literature, some modes and elements (territorial, social, financial) are presented that contribute to creating the conditions for resilience through adaptive heritage reuse according to community-based approaches. Without claiming to be exhaustive, this evidence should be considered in the design phase of resilience programmes, policies or projects related to cultural heritage.Originality/valueThe concepts of community and resilience are becoming increasingly important in the field of cultural heritage. This paper makes a creative contribution to the ongoing debate by presenting and evaluating the contribution of adaptive reuse practices to resilience building.
Effect of TiO2 nanoparticles on the microstructure, mechanical and thermal properties of rapid quenching SAC355 lead-free solder alloy
Authors: Al-sorory Hamed , Gumaan Mohammed S. , Shalaby Rizk Mostafa 
Published: April 2022

Purpose
This study aims to investigate the effect of a small amount of TiO2 NPs addition on the microstructure, thermal, mechanical and electrical properties of environmentally friendly eutectic (SAC355)100-x(TiO2)x (x = 0.1, 0.3, 0.5, 0.7 and 1 wt.%) solder alloys.

Design/methodology/approach
Mechanical, thermal and electrical properties and microstructure conditions are taken into major consideration in any study of materials containing nanoparticles. Dynamic resonance technique, X-ray diffraction and scanning electron microscopy were carried out to study stiffness, identification of the phases and the morphology features of the solder. Structure and microstructure analysis indicated that the presence of rhombohedral β–Sn phase in addition to orthorhombic intermetallic compound (IMC) Ag3Sn and Cu3Sn phase dispersed in Sn matrix. In addition, the results showed that TiO2 NPs addition at a small trace amount into SAC355 system reduces and improves the particle size of both rhombohedral β–Sn and orthorhombic IMC Ag3Sn and Cu3Sn. The interstitial dispersion of TiO2 NPs at grain boundaries resulted in Ag3Sn being more uniform needle-like, which is distributed in the β–Sn matrix. The fine and uniform microstructure leads to improvement of mechanical strength.

Findings
Some important conclusions are summarized as follows: microstructure investigations revealed that the addition of TiO2 NPs particles to eutectic SAC355 inhibited in reducing and refines the crystallite size as well as the Ag3Sn IMC, which reinforced the strength of plain solder alloy. The mechanical properties values such as Young’s modulus and Vickers microhardness of SAC355 solder alloy can be significantly improved by adding a trace amount of TiO2 NPs compared with plain solder because of the existence of appropriate volume fraction of Ag3Sn IMC. The results show that the best creep resistance is obtained when the addition of 0.3 wt.% of TiO2 NPs is compared to plain solder. TiO2 NPs addition could increase the melting temperature, compared with plain solder. All results showed that TiO2 NPs addition is an effective method to enhance new solder joints.

Practical implications
New solder alloys.

Originality/value
Development of TiO2 NPs-doped eutectic SAC355 lead-free solder for electronic packaging.
The place of ISO 18404:2015 in organisational improvement
Authors: Oudrhiri Radouane , Al-Balushi Mustafa , Anwyl Stuart , Bendell Anthony , Chamie Sabet , Coleman Shirley Yvonne , Hayman Mark , Hilton Roger , Melhem Osama Ahmad , Patel Jayeshkumat , Ward Steve , White Simon , Whitehouse Peter 
Published: April 2022

PurposeThis paper gives the background to the ISO 18404:2015 standard and explains its rationale. It aims to correct misconceptions and erroneous statements about the standard appearing in the paper by Antony et al. (2021) and to demonstrate the usefulness of the standard in a wide range of application sectors.Design/methodology/approachA review of recently reported misconceptions and erroneous statements is presented and clarifications are provided. A qualitative interview approach was utilised to obtain the views of leading academics and practitioners familiar with Six Sigma and Lean in a range of sectors and from different parts of the world. This includes the results of a survey for capturing expectations and requirements for the next ISO18404 version.FindingsClarifications were needed to correct some misconceptions and erroneous statements in recently published work. However, on review, the reports of the interviews in Antony et al. (2021) indicate that most Lean Six Sigma professionals have positive experiences with ISO 18404:2015 and see the advantages of a common standard in helping continuous improvement deployment. Possible causes of some reported negative results are already scheduled to be addressed in the forthcoming review of ISO 18404:2015.Research limitations/implicationsA very real constraint when conducting research into ISO 18404:2015 is to obtain a balanced view of the standard from those who have a vested interest in its continuation and evolution, or not. Whilst the authors cannot claim to be any more objective than Antony et al.’s (2021) authors and commentators, they are, in contrast to that group, highly knowledgeable about the reality of the standard, rather than speculating in ignorance.Practical implicationsA very real constraint when conducting research into ISO 18404:2015 is to obtain a balanced view of the standard which is balanced with respect from those who have a vested interest in its continuation and evolution, or not. Whilst the current authors cannot claim to be any more objective than previous authors, Antony et al.’s (2021) authors and commentators, they are, in contrast to that group, highly knowledgeable about the reality of the standard, rather than speculating in ignorance.Originality/valueThe paper gives a clear description of the ISO standard development process and provides a resource for people to obtain insight into the value or non-value add of a standard in Six Sigma and Lean, and the appropriate details of such a standard. These results can form the basis of a case for the implementation of the standard for those organisations currently trying to decide whether or not to implement it.
Social stratification in meaningful work: Occupational class disparities in the United Kingdom
Authors: Williams Mark , Gifford Jonny , Zhou Ying 
Published: April 2022

AbstractSociologists have long been interested in the meaning workers derive from their jobs. The issue has garnered increasing academic and policy attention in recent years with the concept of “meaningful work,” yet little is known about how social stratification relates to access to it. This paper addresses this issue by exploring how the meaningfulness of jobs—as rated by their incumbents—is stratified across classes and occupations in a national survey of 14,000 working adults in the United Kingdom. It finds modest differentials between classes, with those in routine and manual occupations reporting the lowest levels of meaningfulness and those in managerial and professional occupations and small employers and own account workers reporting the highest levels. Detailed job attributes (e.g., job complexity and development opportunities) explain much of the differences in meaningfulness between classes and occupations, and much of the overall variance in meaningfulness. The main exception is the specific case of how useful workers perceive their jobs to be for society: A handful of occupations relating to health, social care, and protective services which cut across classes stand out from all other occupations. The paper concludes that the modest stratification between classes and occupations in meaningful work is largely due to disparities in underlying job complexity and development opportunities. The extent to which these aspects of work can be improved, and so meaningfulness, especially in routine and manual occupations, is an open, yet urgent, question.
Identification of two new recessive MC1R alleles in red‐coloured Evolèner cattle and other breeds
Authors: Hauser Miriam , Signer‐Hasler Heidi , Küttel Luzia , Capitan Aurélien , Guldbrandtsen Bernt , Hinrichs Dirk , Flury Christine , Seefried Franz R. , Drögemüller Cord 
Published: April 2022

AbstractSequence variations in the melanocortin‐1 receptor (MC1R) gene are associated with melanism in different animal species. Six functionally relevant alleles have been described in cattle to date. In a hypothesis‐free approach we performed a genome‐wide allelic association study with black, red and wild‐coloured cattle of three Alpine cattle breeds (Eringer, Evolèner and Valdostana), revealing a single significant association signal close to the MC1R gene. We searched for candidate causative variants by sequencing the entire coding sequence and identified two novel protein‐changing variants. We propose designating the mutant alleles at MC1R:c.424C>T as ev1 and at MC1R:c.263G>A as ev2. Both affect conserved amino acid residues in functionally important transmembrane domains (p.Arg142Cys and p.Ser88Asn). Both alleles segregate predominantly in the Swiss Evolèner breed. They occur in other European cattle breeds such as Abondance and Rotes Höhenvieh as well. We observed almost perfect association between the MC1R genotypes and the coat colour phenotype in a cohort of 513 black, red and wild‐coloured cattle. Animals carrying two copies of MC1R loss‐of‐function alleles or that were compound heterozygous for e, ev1, or ev2 have a red to dark red (chestnut‐like red) coat colour. These findings expand the spectrum of causal MC1R variants causing recessive red in cattle.
COVID‐19 disease and vaccination in pregnant and lactating women
Authors: Safadi Marco A. P. , Spinardi Julia , Swerdlow David , Srivastava Amit 
Published: April 2022

AbstractBackgroundMore than 325,000 cases of coronavirus disease 2019 (COVID‐19) have been reported among pregnant women in the Americas.AimsThis review examines the impact of COVID‐19 in pregnant women and describes available evidence on the safety, effectiveness, and immune response(s) to vaccination among pregnant and lactating women.ContentMultiple studies indicate that pregnant women are more susceptible to adverse COVID‐19 outcomes, including hospitalization, intensive care unit admission, and invasive ventilation than non‐pregnant women with COVID‐19. Furthermore, COVID‐19 in pregnancy is associated with adverse maternal and neonatal outcomes. Adverse COVID‐19 outcomes appear to disproportionately affect pregnant women from low‐ and middle‐income countries, likely reflecting inequities in access to quality healthcare. Despite the absence of safety and efficacy data from randomized clinical trials in this subpopulation, observational studies and data from pregnancy registries thus far have demonstrated that vaccination of pregnant or lactating women against COVID‐19 is safe, effective, and results in robust immune responses including transfer of antibodies to the newborn via the placenta and breast milk, respectively.ImplicationsThese data support vaccination recommendations intending to help protect these vulnerable individuals against COVID‐19 and its sequelae. Randomized clinical studies will further evaluate the safety and immunogenicity of COVID‐19 vaccines in these populations. This review examines the impact of COVID‐19 in pregnant women and describes available evidence on the safety, effectiveness, and immune response(s) to vaccination among pregnant and lactating women.
Association between screening for antenatal depressive symptoms and delivery outcomes: The Born in Queensland Study
Authors: San Martin Porter Macarena A. , Kisely Steve , Salom Caroline , Betts Kim S. , Alati Rosa 
Published: April 2022

BackgroundEvidence shows that depressive symptoms during pregnancy increase the risk of an intervention during delivery (induction, the use of forceps or vacuum, and caesarean sections (CS)). Many women with depression during pregnancy are not identified and therefore will not receive appropriate follow up of their symptoms. We hypothesised that routine screening for depressive symptoms during pregnancy could reduce detrimental consequences of depressive symptoms on delivery outcomes.AimWe explored the association between screening for depressive symptoms during pregnancy and delivery outcomes.Materials and MethodsA cross‐sectional analysis of state‐wide administrative data sets. The population included all women who delivered a singleton in Queensland between the July and December of 2015. Logistic regression analyses were run in 27 501 women (93.1% of the total population) with information in all variables. The following were the main outcomes: onset of labour, CS, instrumental vaginal delivery, and all operative deliveries (including both CS and instrumental vaginal deliveries).ResultsWomen who completed the screening had increased odds of a spontaneous onset of labour (adjusted odds ratio (aOR) 1.18; 95% CI 1.09–1.27) and decreased odds of an operative delivery (instrumental or CS) (aOR 0.88; 95% CI 0.81–0.96). Among women who had a vaginal delivery, those who completed the screening had decreased odds of having an instrumental delivery (aOR 0.84; 95% CI 0.74–0.97). Sensitivity analyses in women who did not have a formal diagnosis of depression showed similar results.ConclusionOur findings suggest that screening may decrease interventions during delivery in women with depressive symptoms.