bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Multi-Centre Prospective Study of the Efficacy and Safety of Alglucosidase Alfa in Chinese Patients With Infantile-Onset Pompe Disease
Authors: Zhu Diqi , Zhu Jiacong , Qiu Wenjuan , Wang Benzhen , Liu Lin , Yu Xiaodan , Ou Zhenheng , Shan Guangsong , Wang Jian , Li Bin , Chen Xiaokang , Liu Cong , Li Zipu , Fu Lijun 
Published: June 2022

Background: A high prevalence of infantile-onset Pompe disease (IOPD) in the Chinese population has been noted, but there are currently no reported clinical trials of enzyme replacement therapy (ERT) for IOPD in this population. The purpose of this study was to evaluate the efficacy and safety of alglucosidase alfa in Chinese patients with IOPD.Materials and Methods: A multicentre, single-arm, prospective, open-label clinical trial was performed at 4 sites in China. Eligible Chinese subjects with IOPD received an infusion of alglucosidase alfa at a dose of 20 mg/kg every 2 weeks for up to 52 weeks. The primary endpoints of clinical efficacy were the survival rate and changes in the left ventricular mass index (LVMI). The safety assessment was based on the incidence of adverse events (AEs).Results: A total of 10 eligible subjects were enrolled in the study. The mean age at the start of ERT was 5.36 ± 1.56 months. Nine subjects had survived after 52 weeks of treatment. One subject discontinued the study and died after mechanical ventilation was withdrawn. The intent-to-treat analysis demonstrated that the survival rate was 90.0% (95% confidence interval: 55.5–99.7%). The mean LVMI at week 52 was 70.59 ± 39.93 g/m2 compared to that of 298.02 ± 178.43 g/m2 at baseline, with a difference of -227.60 ± 155.99 g/m2. All subjects had left ventricular mass (LVM) Z scores &gt;10 at baseline, and eight subjects (80%) achieved Z scores &lt;5 at week 52. No treatment-related AEs were observed, and no AEs led to the discontinuation of treatment.Conclusions: This clinical trial is the first study of ERT for IOPD in China, indicating that alglucosidase alfa has favourable efficacy and safety for the treatment of Chinese patients with IOPD (ClinicalTrials.gov number, NCT03687333).
TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury
Authors: Hu Xuyang , Huang Jinxin , Li Yiteng , Dong Lei , Chen Yihao , Ouyang Fangru , Li Jianjian , Li Ziyu , Jing Juehua , Cheng Li 
Published: June 2022

Following spinal cord injury (SCI), microglia gradually migrate to the edge of the lesion, interweaving around the border of the lesion to form the microglial scar, which performs inflammatory limiting and neuroprotective functions. Recent reports showed that Yes-associated protein (YAP) was expressed in astrocytes and promoted the formation of astrocytic scars, while YAP was not expressed in microglia after SCI. YAP and its paralogue transcriptional coactivator with PDZ-binding motif (TAZ) are transcriptional coactivators, which have a similar functional role as both are negatively regulated by the Hippo signalling pathway. However, the expression and function of TAZ after SCI are unclear. Our research group previously found that Fascin-1 was highly expressed in microglia and promoted migration of microglia after SCI, and that, there was a close regulatory relationship between Fascin-1 and YAP/TAZ. In this study, we demonstrated that TAZ was significantly upregulated and mainly expressed in microglia after SCI, and accumulated in the nuclei of microglia in the spinal cord at 14 days post-SCI. Moreover, TAZ was upregulated and accumulated in the nuclei of anti-inflammatory M2-like (M2-L) polarized or myelin-treated microglia. Additionally, XMU-MP-1 (an inhibitor of the Hippo kinase MST1/2 to active TAZ) promoted the aggregation of microglia around the lesion core, resulting in the formation of microglial scars and the functional recovery of mice after SCI. Our findings also indicated that TAZ promoted microglial migration in vitro. Mechanistically, Fascin-1 interacted with TAZ, which upregulated TAZ expression and induced TAZ nuclear accumulation in microglia to promote microglial migration. These findings revealed that TAZ mediated microglial migration to the edge of the lesion core, promoting the formation of microglial scars and functional recovery after SCI. Moreover, TAZ was downstream of Fascin-1, which positively regulated microglial migration after SCI.
Te Film as a Saturable Absorber for the Mid-Infrared Er3+-Doped ZBLAN Fiber Laser
Authors: Zhang Min , Kang Liu , Ma Zhijian , Yan Peiguang , Hou Shaodong 
Published: June 2022

We demonstrate a Q-switched Er3+-doped ZBLAN fiber laser at 2.8 µm mid-infrared (mid-IR) region achieved by adopting Te as the saturable absorber mirror (SAM). The modulation depth and saturation intensity of the Te-SAM were measured to be ∼7.2% and 10.81 MW∕cm2, respectively. Stable Q-switched laser pulses with the maximum pulse energy of 3.05 µJ and the minimum pulse width of 0.457 µs at the launched pump power of 4.51 W were obtained. Maximum average output power of 357 mW with repetition rate of 116.98 kHz were achieved. The signal-to-noise ratio (SNR) is 52 dB, which is higher than that of most 2.8 µm mid-infrared Q-switched fiber lasers reported so far. To the best of our knowledge, this is the first demonstration from a Q-switched fiber laser at 2.8 µm based on a Te-SAM.
Modification of Fibronectin by Non-Enzymatic Glycation Impairs K+ Channel Function in Rat Cerebral Artery Smooth Muscle Cells
Authors: Yang Yan , Nourian Zahra , Li Min , Sun Zhe , Zhang Liping , Davis Michael J. , Meininger Gerald A. , Wu Jianbo , Braun Andrew P. , Hill Michael A. 
Published: June 2022

Fibronectin (FN) enhances K+ channel activity by integrin-mediated mechanisms. As vascular smooth muscle (VSM) K+ channels mediate vasodilation, we hypothesized that modification of fibronectin, via advanced non-enzymatic glycation, would alter signaling of this extracellular matrix protein through these channels. Bovine FN (1 mg/ml) was glycated (gFN) for 5 days using methylglyoxal (50 mM), and albumin was similarly glycated as a non-matrix protein control. VSM cells were isolated from rat cerebral arteries for measurement of macroscopic K+ channel activity using whole cell patch clamp methodology. Pharmacological inhibitors, iberiotoxin (0.1 μM) and 4-aminopyridine (0.1 mM), were used to identify contributions of large-conductance, Ca2+-activated, K+ channels and voltage-gated K+ channels, respectively. Compared with baseline, native FN enhanced whole cell K+ current in a concentration-dependent manner, whereas gFN inhibited basal current. Furthermore, native albumin did not enhance basal K+ current, but the glycated form (gAlb) caused inhibition. gFN was shown to impair both the Kv and BKCa components of total macroscopic K+ current. Anti-integrin α5 and β1 antibodies attenuated the effects of both FN and gFN on macroscopic K+ current at +70 mV. Consistent with an action on BKCa activity, FN increased, whereas gFN decreased the frequency of spontaneous transient outward current (STOCs). In contrast, gAlb inhibited whole cell K+ current predominantly through Kv, showing little effect on STOCs. A function-blocking, anti-RAGE antibody partially reversed the inhibitory effects of gFN, suggesting involvement of this receptor. Further, gFN caused production of reactive oxygen species (ROS) by isolated VSMCs as revealed by the fluorescent indicator, DHE. Evoked ROS production was attenuated by the RAGE blocking antibody. Collectively, these studies identify ion channel-related mechanisms (integrin and ROS-mediated) by which protein glycation may modify VSMC function.
The Perceived Impact of COVID-19 on Functional Activities Among Canadian Education Workers: A Cross-Sectional Study
Authors: Serrano Frances , Nowrouzi-Kia Behdin , Oddson Bruce , Bishai Rita , Casole Jennifer , Gohar Basem 
Published: June 2022

ObjectiveThis cross-sectional study examined the self-perceived impact of the COVID-19 pandemic on 2,378 education workers in Ontario, Canada, during the second wave.MethodsWe examined six domains of functioning as per the short version of the World Health Organization Disability Assessment Schedule-2.0. Participants selected if their functioning had improved, remained unchanged or worsened during the pandemic for each item.ResultsEducational workers described a general worsening of functional activities since the beginning of the pandemic. Moderate-to-extreme challenges were reported for all six functional domains. These challenges appeared to aggravate functional challenges for workers with disability, as indicated by pre-existing work accommodations. Older participants reported worse mobility than younger participants; however, they appeared to have better coping skills in learning new tasks and maintaining friendships. Women were more likely to report difficulties in maintaining household responsibilities.ConclusionsWe consider the role of mental health challenges and pre-existing inequality as predictors of pandemic-related difficulties. Recommendations include more longitudinal research in this population and policymakers to incorporate a health promotion lens to support their education workers more proactively.
Bulk RNA Sequencing With Integrated Single-Cell RNA Sequencing Identifies BCL2A1 as a Potential Diagnostic and Prognostic Biomarker for Sepsis
Authors: Li Jun , Zhou Mi , Feng Jia-Qi , Hong Soon-Min , Yang Shao-Ying , Zhi Lang-Xian , Lin Wan-Yi , Zhu Cheng , Yu Yue-Tian , Lu Liang-Jing 
Published: June 2022

BackgroundSepsis is one of the leading causes of morbidity and mortality worldwide in the intensive care unit (ICU). The prognosis of the disease strongly depends on rapid diagnosis and appropriate treatment. Thus, some new and accurate sepsis-related biomarkers are pressing needed and their efficiency should be carefully demonstrated.MethodsDifferential expression analysis and weighted gene co-expression network analysis (WGCNA) were applied to detect sepsis and monocyte/macrophage-related genes. Least absolute shrinkage and selection operator (LASSO) and random forest regression analyses were used in combination to screen out prognostic genes. Single-cell RNA sequence profiling was utilized to further verify the expression of these genes on a single cell level. Receiver operating characteristic (ROC) curve and decision curve analysis (DCA) were also applied to verify the diagnostic value of the target biomarkers.ResultsThe intersections of the genes detected by differential expression and WGCNA analyses identified 141 overlapping candidate genes that were closely related to sepsis and macrophages. The LASSO and random forest regression analyses further screened out 17 prognostic genes. Single-cell RNA sequencing analysis detected that FCGR1A and BCL2A1 might be potential biomarkers for sepsis diagnosis and the diagnostic efficacy of BCL2A1 was further validated by ROC curve and DCA.ConclusionsIt was revealed that BCL2A1 had good diagnostic and prognostic value for sepsis, and that it can be applied as a potential and novel biomarker for the management of the disease.
Bacillus subtilis Produces Amino Acids to Stimulate Protein Synthesis in Ruminal Tissue Explants via the Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Beta–Serine/Threonine Kinase–Mammalian Target of Rapamycin Complex 1 Pathway
Authors: Wang Qiuju , Ren Yulong , Cui Yizhe , Gao Bingnan , Zhang Hao , Jiang Qianming , Loor Juan J. , Deng Zhaoju , Xu Chuang 
Published: June 2022

BackgroundBacillus subtilis is a probiotic strain that is widely used as a feed supplement for ruminants. In this study, one B. subtilis strain isolated from the ruminal fluid of Holstein dairy cows was used for an ex vivo study with ruminal tissue explants. The main goal was to assess the potential endosymbiotic links between B. subtilis and the ruminal epithelium using molecular analyses and amino acid profiling. The explant culture protocol was first optimized to determine the ideal conditions in terms of tissue viability before performing the actual experiments involving active and inactive bacteria with or without protein synthesis inhibitors, such as LY294002 (phosphatidylinositol 3-kinase inhibitor) or rapamycin [mammalian target of rapamycin (mTOR) inhibitor].ResultsThe mRNA levels of phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta (PIK3CB), serine/threonine kinase (AKT), mTOR, P70S6K1, and eukaryotic translation initiation factor 4E binding protein 1 were the highest (p &lt; 0.01), while those of programmed cell death 4 were the lowest when the tissue was incubated with 107 of B. subtilis. Compared with the inactivated bacteria, the expression levels of PIK3CB and AKT, and overall changes in mTOR and P70S6K1 were greater in rumen explants with living bacteria (p &lt; 0.05). With an increase in B. subtilis concentration, the trends of protein and corresponding gene changes were consistent. There were differences in the concentrations of individual amino acids in the supernatants of living and inactivated bacterial culture groups, with most amino acids enriched in pathways, such as aminoacyl tRNA biosynthesis, cyanoamino acid metabolism, monobactam biosynthesis, or glycine, serine, and threonine metabolism. The addition of psilocybin upregulated the expression levels of PIK3CB and AKT. A significant decrease (p &lt; 0.05) in PIK3CB and mTOR protein expression levels was detected after the addition of LY294002 and rapamycin. In addition, These responses were associated with the downregulation (p &lt; 0.05) of AKT and P70S6K protein expression levels.ConclusionsWe confirmed that the in vivo ruminal tissue culture system is a suitable model for studying probiotic-induced alterations in tissue function. As such, this study provides a means for future mechanistic studies related to microbial regulation and the dietary supply of proteins. In addition, living and inactivated B. subtilis can promote protein synthesis in ruminal tissue explants by altering the expression levels of related factors in the PIK3CB–AKT–mTORC1 pathway, which could further aid in optimizing the feed efficiency and increasing the use of inactivated bacteria as additives in dairy cow farming.
Biological and Social Change of the Aayar Ethnic Group in the Keethari Novel
Authors: R Vimala Devi 
Published: June 2022

Novels are one of the mainstays of contemporary media documenting the biographies of soil-based peoples. Contemporary writers have documented people's lives, customs, rituals, values, in their novels with a contemporary character. The industrial revolution, the Green Revolution, the White Revolution, the deforestation for development projects, the occupation of agricultural lands, the loss of livelihoods to the people living in their respective areas as refugees, the loss of their own land due to land occupation. Their work records the changes in society during the respective eras, such as the transformation of wages into agricultural wages and the loss of livelihood and suicide. Sundara Ramasamy's Oru Puliyamarathin Kathai, Vairamuthu's Kallaikattu epic, work in novels that record the history of the community or a component of a race. S. Tamilchelvi has recorded the lives of the common people who migrate for their livelihood as sheep migrated for their livelihood as evidenced by Kovalan's complaint to chase the prey for livelihood through the sociological approach in the novel. The writers have written extensively on the unique cultural life style of the Mullai people, the people of Mullai land, from the Tolkappiyam to the novel literature up to today. The purpose of this article is to explore the impact of globalization, liberalism, capitalism, the astounding growth of consumer culture and natural disasters on the livestock breeding and availability of the ancient Aayar.
Texto, memória e subjetividade: uma leitura de "Aperfeiçoamento o imperfeito", de Carla Luma
Authors: Soares Isabelle Maria 
Published: June 2022

“Aperfeiçoando o imperfeito” de Carla Luma, conto escolhido como objeto de estudo do presente artigo, é um texto literário situado no espaço virtual. Publicado no blog Escritoras Suicidas, o conto instigou a seguinte proposta: uma leitura pela ótica de alguns conceitos acerca do texto (BARTHES, 2004; KARVALHAL, 2012; CARVALHAL, 1992; SAMOYAULT, 2008), da memória (NORA, 1993; BERGSON, 1999; HALBWACHS, 2015; POLLAK, 1989, 1992) e da subjetividade (GIDDENS, 1991; HALL, 2015; CASTORIADIS, 1999). A abordagem do aporte teórico é colocada em prática a partir da nossa análise do conto de Luma. Percebemos, assim, que o texto literário estudado dialoga com outros textos por meio de uma pluralidade de memórias e da subjetividade fragmentada da voz que narra.
SGINet: Toward Sufficient Interaction Between Single Image Deraining and Semantic Segmentation
Authors: Wei Yanyan , Zhang Zhao , Zheng Huan , Hong Richang , Yang Yi , Wang Meng 
Published: June 2022

<p>Please cite this work as </p>
<p><br></p>
<p>Yanyan Wei, Zhao Zhang, Huan Zheng, Richang Hong, Yi Yang and Meng Wang, "SGINet: Toward Sufficient Interaction Between Single Image Deraining and Semantic Segmentation," In: Proceedings of the 30th ACM International Conference on Multimedia (ACM MM), Lisbon, Portugal, June 2022. </p>
Distributed Precoding For Decentralized Estimation In Coexisting IoT Networks
Authors: Garg Navneet , Ratnarajah Tharmalingam , Vakamulla Venkata Mani , Sellathurai Mathini 
Published: June 2022

<p>With the increasing number of applications of internet-of-things (IoT) devices, co-channel interference is unavoidable among coexisting wireless networks, where multiple IoT devices transmit their observations to their respective des tinations (access points (APs)). In this scenario, we present joint precoding and power allocation solution to minimize the mean squared error (MSE), while satisfying the power constraint at each IoT device. In this regard, first, the necessary conditions for the feasibility for the joint problem is obtained for successful interference mitigation. Subsequently, an MSE minimization based iterative algorithm is derived and analyzed for convergence. Analysis shows that the MSE performance or the signal-to-noise-plus-interference-ratio (SINR) at the APs is limited by the observation signal-to-noise-ratio (SNR). It is also inferred that to avoid the MSE saturation at AP, the transmit power at IoT devices should be scaled proportional to and must be less than the observation SNR. We compare the performance of our solution with two classical methods, namely, the minimum variance distortionless precoding (MVDP) and interference alignment (IA) methods, which are modified for the given coexisting scenario. Simulations verify the above results, and show that MSE based algorithm converges globally with robustness to initializations and provides the better precoders and power allocation as compared to MVDP’s and IA’s.</p>
Noncooperative Equilibrium Seeking in Distributed Energy Systems Under AC Power Flow Nonlinear Constraints
Authors: Scarabaggio Paolo 
Published: June 2022

<p>Power distribution grids are commonly controlled through centralized approaches, such as the optimal power flow.</p>
<p>However, the current pervasive deployment of distributed renewable energy sources and the increasing growth of active players, providing ancillary services to the grid, have made these centralized frameworks no longer appropriate.</p>
<p>In this context, we propose a novel noncooperative control mechanism for optimally regulating the operation of power distribution networks equipped with traditional loads, distributed generation, and active users. The latter, also known as prosumers, contribute to the grid optimization process by leveraging their flexible demand, dispatchable generation capability, and/or energy storage potential.</p>
<p>Active users participate in a noncooperative liberalized market designed to increase the penetration of renewable generation and improve the predictability of power injection from the high voltage grid.</p>
<p>The novelty of our game-theoretical approach consists in incorporating economic factors as well as physical constraints and grid stability aspects.</p>
<p>Lastly, by integrating the proposed framework into a rolling-horizon approach, we show its effectiveness and resiliency through numerical experiments.</p>
<p><br></p>
<p> </p>
<p>Postprint accepted for pubblication in <em>IEEE Transactions on Control of Network Systems</em></p>
<p><br></p>
<p><strong>How to cite</strong>: P. Scarabaggio, R. Carli and M. Dotoli, (2022) "Noncooperative Equilibrium Seeking in Distributed Energy Systems Under AC Power Flow Nonlinear Constraints," in <em>IEEE Transactions on Control of Network Systems</em>.</p>
<p>DOI: <a href="https://doi.org/10.1109/TCNS.2022.3181527" target="_blank">https://doi.org/10.1109/TCNS.2022.3181527</a><br>
</p>
The Influence of Consumer Behavior and Product Quality on Consumer Decision Making in Selecting Dana as Financial Technology Mobile Payment Application
Authors: Raya Agung , Kartawinata Budi Rustandi 
Published: June 2022

As technology advances and becomes more sophisticated, consumer behavior patterns shift. The presence of such events stimulates diverse corporate industries to compete to develop innovation. Businesses in the field of financial technology also use it (Fintech). DANA is a mobile payment application that supports ATM cards for online purchases. Although DANA is a new program, it is capable of adapting fast to attract users. DANA has proven to be the third most popular app in Indonesia, with the most users and the most downloads. The goal of this research is to analyze the influence of customer behavior and product quality on the selection to use DANA as a mobile payment application. This type of research is quantitative by using descriptive data analysis techniques that use multiple linear hypothesis models with a total of 400 research samples. The results obtained from this study that consumer behavior and quality product have a significant and positive effect on partial (individual) decision making. As the results of simultaneous testing (collectively) on consumer behavior and product quality to consumer decision making in choosing DANA as a mobile payment application has a significant effect and the results are accepted.