bluuebunny/crossref_metadata_2025_split huggingface dataset:
New diagnosis for hepatocellular carcinoma via hsa_circ_0097009
Authors: Wang Xue , Peng Tingwei , Xu Limin , Lu Zuohua , Liu Qianqian , Li Beibei , Wang Yufang , Liu Xinghui 
Published: November 2022

Abstract
Background
Although circular RNAs take a key part in the regulation of cancer, its role in the diagnosis and prognosis of hepatocellular carcinoma (HCC) still unclear. This study investigated the diagnostic and prognostic usefulness of hsa_circ_0097009 in HCC.
Methods
qRT-PCR assay was performed to detect the presence of hsa_circ_0097009 in plasma collected from 30 HCC patients and 30 healthy controls. Receiver operating characteristic (ROC) curve, Cox regression, and Kaplan–Meier curve analyses were applied to evaluate the clinical diagnostic and prognostic values of hsa_circ_0097009.
Results
The experimental data suggested that hsa_circ_0097009 expression of HCC plasma and cell lines is upregulated. The plasma hsa_circ_0097009 level of HCC patients was linked to tumor diameter (P = 0.026) and alpha-fetoprotein (AFP) expression level (P = 0.019). In addition, the plasma level of hsa_circ_0097009 decreased markedly after surgery to remove the tumor. Moreover, the level of plasma of hsa_circ_0097009 in HCC patients was increased compared with healthy controls (P < 0.0001) and hepatitis B patients. ROC curve reflected that the area under the curve (AUC) of plasma hsa_circ_0097009 to distinguish HCC from healthy controls and hepatitis B patients were 0.9368 (95 percent CI = 0.8874–0.9862) and 0.9067 (95 percent CI = 0.8439–0.9695), respectively, with the same sensitivity 0.9779 and the same specificity 0.6889. Furthermore, the AUC value of hsa_circ_0097009 combined with AFP for distinguishing HCC from healthy controls was 0.9822 (95 percent CI = 0.9682–0.9961), with the sensitivity 0.9889 and specificity 0.667. The plasma hsa_circ_0097009 low expression group had a considerably longer overall survival (OS) than the high expression group.
Conclusion
The study suggests that hsa_circ_0097009 could be used as a new potential biomarker in diagnoses and prognoses of HCC patients.
The principle of beneficence in nursing care: an integrative review
Authors: Valizadeh Leila , Zamanzadeh Vahid , Cheraghi Rozita , Jafarzadeh Anahita 
Published: November 2022

Abstract
Background: Ethics-based nursing practice can transform health care practices. As the biggest human capital in the health care system, nurses are obliged to follow the ethical principles in this field. One of these ethical principles; is beneficence, which is considered the core of nursing care. This study aimed to investigate the ethical principle of beneficence in nursing care and its related challenges.
Methods: This integrative review was conducted using the Whittemore & Knafl method in 5 stages, including problem identification, searching the literature, evaluating primary sources, analyzing data, and presenting the results. Databases like SID, Irandoc, Magiran, Google Scholar, Web of science, PubMed, and Scopus were searched using the keywords; "beneficence", "ethic", "nursing" and "care" in English and Persian. After applying inclusion criteria and assessing the articles using Bowling's Quality Assessment Tool, finally, 16 papers were included from 957.
Results: After reviewing and evaluating the qualified articles, the findings were classified into four main categories: 1) nature, 2) applicability, 3) Relevant and influential factors, and 4) challenges related to the ethical principle of beneficence in nursing care.
Conclusion: Based on the results of this review it seems that paying attention to training to the principle of beneficence in nursing care can provide positive outcomes for patients to benefit from this principle and finally, it leads to increasing the well-being and health of patients, reducing their mortality rate, increasing satisfaction and maintaining the respect and human dignity of patients.
Sexual dysfunction worsen both the general and specific quality of life of women with irritable bowel syndrome. A cross-sectional study
Authors: Camacho Santiago , Díaz Andrea , Pérez Paulina , Batalla Héctor , Flores Yoshua , Altamirano Evelyn , Tijera María de Fátima Higuera-de la , Murguía Daniel , Gómez-Laguna Laura 
Published: November 2022

Abstract
Background: Irritable bowel syndrome (IBS) and sexual dysfunction (SxD) lowers quality of life (QOL) separately, but the effect of their overlap in unselected populations has not been studied.
Objective: To evaluate the QOL of IBS women with and without SxD and compare it with controls.
Methods: In this cross-sectional assessment, we studied 51 IBS women (Rome IV criteria) and 54 controls. SxD was determined using the female sexual function index questionnaire. QOL was evaluated by the Short Form 36 (SF-36) and IBS-QOL questionnaires.
Results: SxD prevalence was similar between IBS women (39.22%) and controls (38.89%). Compared with other groups, IBS patients with SxD showed lower scores in all domains as well as in the physical, mental summaries of the SF-36 and almost all domains (except for body image, food avoidance, and social reaction compared with IBS patients without SxD) and the total score of IBS-QOL.
Conclusions: These findings show that SxD worsens both general and specific QOL of women with IBS. The consideration of SxD in patients with IBS will allow us to make a more effective diagnostic and therapeutic approach. Clinical trial registry in Mexico City General Hospital: DI/19/107/03/080. Clinical trials registration: NCT04716738.
Over-coupled resonator for broadband surface enhanced infrared absorption (SEIRA)
Authors: Paggi Laura , Fabas Alice , Ouazzani Hasnaa El , Hugonin Jean-Paul , Bardou Nathalie , Dupuis Christophe , Greffet Jean-Jacques , Bouchon Patrick 
Published: November 2022

Abstract
Detection of molecules is a key issue for many applications. Surface enhanced infrared absorption (SEIRA) uses arrays of resonant nanoantennas with good quality factors which can be used to locally enhance the illumination of molecules. The technique has proved to be an effective tool to detect small amount of material. However the use of nanoresonators only enables the detection of a few absorption lines, hampering the identification of the spectral fingerprint of a molecule. Here, we introduce a new paradigm and use low quality factor resonators with large radiative losses (over-coupled resonators). The bandwidth enables to detect all absorption lines between 5 and 10 μm. Counterintuitively, despite a lower quality factor, the system sensitivity is improved and we report a reflectivity variation as large as one percent per nanometer of molecular layer of PMMA. This paves the way to specific identification of molecules. We illustrate the potential of the technique with the detection of the explosive precursor dinitrotoluene (DNT). We compare the data with numerical simulations and introduce an analytic model of the results.
Beyond single-crystalline metals: ultralow-loss silver films on lattice-mismatched substrates
Authors: Baburin Aleksandr S. , Moskalev Dmitriy O. , Lotkov Evgeniy S. , Sorokina Olga S. , Baklykov Dmitriy A. , Avdeev Sergey S. , Buzaverov Kirill A. , Yankovskii Georgiy M. , Baryshev Alexander V. , Ryzhikov Ilya A. , Rodionov Ilya A. 
Published: November 2022

Abstract
High-quality factor plasmonic devices are crucial components in the fields of nanophotonics, quantum computing and sensing. The majority of these devices are required to be fabricated on non-lattice matched or transparent amorphous substrates. Plasmonic devices quality factor is mainly defined by ohmic losses, scattering losses at grain boundaries, and in-plane plasmonic scattering losses of a metal – substrate system. Here, we demonstrate the deposition technique to e-beam evaporate ultralow-loss silver thin films on transparent lattice-mismatched substrates. The process is based on evolutionary selection growth. The key feature of our approach is a well-defined control of deposition on a cooled substrate, self-crystallization and subsequent annealing for precise stress relaxation that promote further grains growth. We are able to deposit 100-nm thick ultraflat polycrystalline silver films with micrometer-scale grains and ultralow optical losses. Finally, we show ultra-high-quality factor plasmonic silver nanostructures on transparent lattice-mismatched substrate comparable to epitaxial silver. This can be of the great interest for high performance or single-molecule optical sensorics applications.
Construction of a Bacteriophage-Derived Vector with Potential applications in Targeted Drug Delivery and Cell Imaging
Authors: Sharifi Mehdi , Alizadeh Ali Akbar , Hamzeh-Mivehroud Maryam , Dastmalchi Saivoush 
Published: November 2022

Abstract
There is a strong relation between dysregulation of epidermal growth factor receptor (EGFR) and the development of epithelial-derived cancers. Therefore, EGFR has usually been considered the desired target for gene therapy. Here, we propose an approach for targeting EGFR expressing cells by phage particles capable of displaying EGF and GFP as the tumor-targeting and reporting elements, respectively. For this, the superfolder GFP-EGF (sfGFP-EGF) coding sequence was inserted at the N-terminus of the pIII gene in pIT2 phagemid. The capability of constructed phage to recognize EGFR overexpressing cells was monitored by fluorescence microscopy, fluorescence-activated cell sorting (FACS), and cell-based ELISA experiments. The FACS analysis showed a significant shift in the mean fluorescence intensity (MFI) of the cells treated with phage displaying sfGFP-EGF compared to phage displaying only sfGFP. The binding of phage displaying sfGFP-EGF to A-431 cells, monitored by fluorescence microscopy, indicated formation of sfGFP-EGF-EGFR complex on the surface of the treated cells. Cell-based ELISA experiments showed that the phages displaying either EGF or sfGFP-EGF can specifically bind EGFR expressing cells. The vector constructed in the current study has the potential to be engineered for gene delivery purposes as well as cell-based imaging for tumor detection.
Diversity, genetic structure and core collection of mangaba (Hancornia speciosa) genebank
Authors: Silva Ana Veruska Cruz , Gois Itamara Bomfim , Soares Adrielle Naiana Ribeiro , Ledo Ana Silva 
Published: November 2022

Abstract
This work was developed with the objective of characterizing the variability and genetic structure of matrices and progenies of the Mangaba genebank of Embrapa Tabuleiros Costeiros, to define a core collection. From the matrix of seven accessions, 289 individuals were generated, evaluated by nine SSR markers. The characterization of genetic variability was performed from the estimates: Average number of alleles observed (Na = 5.63) and effective (Ne = 2.71); Shannon Information Index (I = 1.04); Heterozygosity observed (Ho = 0.45) and expected (He = 0.51); Fixation index (f = 0.13); Percent polymorphism (%P = 90.47); and, Number of private alleles (Nap = 20). The Analysis of Molecular Variance (AMOVA) identified that the largest proportion of genetic variation is present within accessions (92%). The estimates of genetic differentiation (GST and RST) were considered low (< 0.05) to moderate (0.05 to 0.15) magnitude. Multivariate analyses (PCoA, Rogers and Bayesian genetic diversity) did not discriminate the accessions according to origin. The formation of the core collection allowed retaining 94.90% of the identified alleles. The accessions present genetic variability to be explored in mangaba conservation and genetic improvement programs.
Integrated analysis of m6A regulator-mediated RNA methylation modification patterns and immune characteristics in Sjögren’s Syndrome
Authors: Yin Junhao , Fu Jiayao , Xu Jiabao , Chen Changyu , Zhu Hanyi , Zhao Yijie , Zheng Zhanglong , Wang Baoli , Zheng Lingyan 
Published: November 2022

Abstract
Growing evidence suggests that N6-methyladenosine (m6A), the most abundant RNA internal modification, plays a critical role in immune regulation and thereby potentially contributes to the pathogenesis of autoimmune disorders. However, the role of m6A modification of the immune microenvironment of Sjögren’s syndrome (SS) remains unknown. In this study, we used data from public databases and our sequencing efforts to evaluate the expression levels of m6A regulators by profiling the data of whole peripheral blood of 220 SS patients and 62 healthy controls. We found that SS was associated with the expression of several m6A regulators, and this difference was correlated with activated CD4+T cells. We screened key genes with a random forest (RF) machine learning algorithm and constructed a diagnostic model of SS using multivariate logistic regression analysis. Two distinct m6A modification patterns were determined by unsupervised clustering, with significant differences in immunocyte infiltration, immune reactivity, and enriched biological functions. Key m6A regulators, gene modules, and co-expression networks of m6A-related genes were identified by conventional bioinformatics methods. This identified three key m6A regulators (METTL3, ALKBH5, and YTHDF1) and two m6A-related hub genes (COMMD8 and SRP9) which may play an essential role in the diagnosis and treatment of SS. This study demonstrates the close relationship between m6A modification and the immune microenvironment in SS and provides a basis for an improved understanding of m6A modification patterns and the exploration of new therapeutic options for SS.
Urban Population Characteristics Benchmarking Inequity of Access to Public Transport Services in Ibadan, Nigeria
Authors: Ajayi Julius O. , Osabohien Romanus , Adebayo Iyabo T. , Wojuade Christopher A. , Aworemi Joshua R. 
Published: November 2022

Abstract

Inequity in access to public transport services highlights the differences in the ease and difficulty in reaching destinations and affects the quality of urban life. The study examined the factors underlying Public Transport Service Accessibility Inequity (PTAI) of population groups and asked if inequity of accessibility to public transport services differs based on the travel needs of aggregated population groups. Incidental-random sampling technique was used to select 400 participants for a questionnaire survey in ten major transit stops in Ibadan. Over a period of five (5) working days, 40 commuters were engaged in each of the transit stops. Descriptive statistics was employed to summarize the responses from the sampled respondents. One-way Analysis of Variance (ANOVA) was used to test if the PTAI of aggregated population groups differs based on their travel needs at a statistical probability of 5% level of significance. The findings of the study revealed that public transport services are inadequate for the low-income groups. More so, PTAI differ based on the travel needs/purposes of aggregated population groups and low-income people who use public transport to access essential activities such as work and education experience a higher levels of public transport accessibility inequity than those who use public transport to access other activities which are either domestic, social or leisure. Government and policymakers should therefore prioritize the accessibility of low-income groups whose travel purposes are majorly work and education to public transport by formulating strategic policies which addresses their peculiar transport needs.
Urban Stream Corridors and Forest Patches – The Connections: A Case Study of Bloomington, IN
Authors: Luchauer Gretchen , Freeman-Day Stephanie , Fischer Burnell 
Published: November 2022

Abstract
Streams and forests are connected ecosystems, but few studies have looked at the connectivity between streams and forests in urban environments. City-made decisions affect connections between streams and forests by isolating both streams and forests. Streams are often channelized or buried to reduce flooding and increase potential developable areas. Forests become fragmented and are removed unless they are protected by public ownership, private ownership, or mixed governance arrangements. Historical choices in land usage affect the sites and sizes of current urban streams, forests, and development. This affects the distribution of impervious surfaces, a ubiquitous urban land cover type, which separates streams from forests. Despite these barriers to stream/forest interactions, cities can experience stream/forest connectivity. Seven Bloomington watersheds are ranked on their proportions of buried streams, channelized streams, forested hydrology, forested streams, urban forest patch cover, and impervious surface cover, along with historical presence of urbanization. Several watersheds demonstrate stream/forest connectivity, with five of these watersheds containing 50% or greater forested stream segments. Bloomington canopy cover reduces stormwater runoff by approximately 127 kiloliters per year. These forested areas reduce flooding, reduce nutrient loading, and reduce stream conditions associated with urban stream syndrome. Streams provide incentive for forest protection, aesthetic value, and recreational value. Understanding urban stream/forest connectivity can improve green infrastructure design and green space design, which improves urban resilience and better connects residents to the environment.
Immunomodulatory effects of β-defensin 2 on tumor-associated macrophages induced antitumor function in breast cancer
Authors: Agarwal Sonam , Chauhan Anita , Singh Khushwant , Kaur Rupinder , Masih Marilyn , Gautam Pramod Kumar 
Published: November 2022

AbstractBackground: TAMs express cytokines and chemokines that can suppress antitumor immunity and promote tumor progression. The immunomodulatory and antitumor function of β-defensin 2 is still unclear, despite the evidence of infection response. So, this study aims to investigate the association between β-defensin 2 and TAMs and determine the role in tumor-promoting attributes of TAMs reversal of phenotype in tumor regression.Methods:Swiss albino mice and C127i breast cancer cell line were used in this study. C127i conditioned media was prepared and generated macrophage-derived TAM to study antitumor function. Flow cytometry was performed for phenotype identification of macrophages and TAMs. MTT was performed to estimate cytotoxicity and dose optimization of β-defensin 2. Oxidative stress was analyzed by H2O2and NO estimation, and qPCR was performed for iNOS, cytokines and chemokines quantification.Results:PEC harvested macrophages were characterized by flow-cytometry using F4/80, CD11c antibodies with 98% pure population of macrophages and cultured in C127i conditioned media for 7 days. TAM markers were estimated, and it was found that 98 % expression of F4/80, CD-206, and CD-115 expression compared to macrophages. Purified 100ng/ml of β-defensin 2 was used to stimulate the TAMs 98% population was viable, which was confirmed by cell viability assay. ROS levels decreased (TAMs alone p<0.05, TAMs treated p<0.001) compared to control. IL-6, IL-10, IL-3, TNF-α, and TGF-β and CXCL-1, CXCL-5, CXCL-15, CCL-24, and CCL-5 decreased drastically compared to control.Conclusion:This is the first report of β-defensin 2 on TAMs to elucidate the immunomodulatory and anti-tumor function. It was found that the cytokines and chemokines molecules, ROS expression, pliably changed, which facilitates tumor regression. β-defensin 2 is a new therapeutic target peptide to revert tumor-promoting function.
The use of forced oscillation technique for monitoring of lung mechanics during covid-19 pneumonia
Authors: Taivans Immanuels , Grima Laura , Zvaigzne Ligita , Jurka Normunds , Gordjusina Valentina , Strazda Gunta 
Published: November 2022

Abstract
Forced oscillation technique (FOT) is non-invasive method, that does not require the patient's participation and allows to evaluate lung and airways mechanics. The question addressed by the study is whether Computerized Tomography (CT) and FOT data correlate between and whether FOT allows to follow up the course of covid-19 pneumonia during the acute intrahospital and recovery period.
Material, patients and methods
In the study were involved 30 intrahospital treated spontaneously breathing, oxygen dependent patients with moderate severity SARS-CoV-2 induced pneumonia. (CT) scans were quantitatively assessed by CT-scoring method. During the hospital stay and 3 months after the discharge lung function was measured by FOT method.
Results
During acute period resistance values R5 and R5-20 were elevated above the upper limit of normal (ULN) in 28% and 50% of all patients respectively. Reactance indices X5, AX and Fres exceeded ULN in 55%, 68% and 66% of cases. Significant correlations were observed between PaO2/FiO2, the time spent in the hospital and R5, X5, AX and Fres expressed as % of predicted. Significant correlations between FOT indices and CT-score was not found. After 3 months 18 patients were compared. R5-20 from 316;-171/+583 decreased to 122;- 56/+266 % pred. P=0,0075. AX from 448;- 275/+731 decreased to 213;- 131/+347 % pred,p=0,0112 and Fres from 171;- 143/+200 to 130;- 110/+150 %pred, p=0,0081.
Study has shown that FOT method reliably reflects the pathological changes in lung mechanics and allows to monitor the course of covid-19 disease, but it cannot substitute for CT.
Real-time Driver Cognitive Strain Evaluation System Based on Multivariate Biosignal Analysis
Authors: Shimizu Takeshi , Shima Keisuke , Mukaeda Takayuki , Muraji Shu , Matsuo Juntaro , Horiue Masayoshi 
Published: November 2022

Abstract
Traffic accidents are often attributed to reduced attentional capacity, as vehicle operations involve complex tasks that depend on the driver's mental workload (MWL). Cognitive strain in MWL affects focus on other vehicles and pedestrians, which can result in serious accidents. Therefore, a method for effective cognitive-strain evaluation is required.
In this study, the driver cognitive strain was determined using the a posteriori probability method. The posteriori probability of the driver strain state is computed using multidimensional biological signals mapped into a probabilistic space based on a Gaussian mixture model. Ultimately, the driver’s cognitive strain was estimated using a regression function based on the posteriori probability. In the experiments, tasks tracked the preceding vehicle, and N-back evaluation helped elucidate the cognitive strain. Other experiments have shown an increase in the evaluation value during the satnav operation. Accordingly, the proposed method is considered suitable for real-time driver cognitive strain evaluation.