bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Electrical Stimulation on Spinal Cord Injury: In Vitro and In Vivo Analysis
Authors: Bang Woo-Seok , Han Inbo , Mun Seul-Ah , Cho Dae-Chul , Lee Sang-Woo , Kim Byoung-Joon , Kim Chi Heon , Choi Hyuk , Kim Kyoung-Tae 
Published: June 2022

Abstract
Electrical stimulation influences neural stem cell neurogenesis. We analyzed the effects of electrical stimulation on neurogenesis in rodent spinal cord-derived neural stem cells (SC-NSCs) in vitro and in vivo and evaluated functional recovery and neural circuitry improvements with electrical stimulation using a rodent spinal cord injury (SCI) model. Rats (20 rats/group) were assigned to a sham (Group 1), SCI only (Group 2), SCI + electrode implant without stimulation (Group 3), and SCI + electrode with stimulation (Group 4) groups to count total SC-NSCs and differentiated neurons and evaluate morphological changes in differentiated neurons. Further, the Basso, Beattie, and Bresnahan scores were analyzed, and the motor and somatosensory evoked potentials in all rats were monitored. In vitro, biphasic electrical currents increased SC-NSC proliferation and neuronal differentiation and caused qualitative morphological changes in differentiated neurons. Electrical stimulation promoted SC-NSC proliferation and neuronal differentiation and improved functional outcomes and neural circuitry in SCI models. Increased Wnt3, Wnt7, and β-catenin protein levels were also observed after electrical stimulation. In conclusion, our study proved the beneficial effects of electrical stimulation on SCI. We believe that Wnt/β-catenin pathway activation may be associated with this relationship between electrical stimulation and neuronal regeneration after SCI.
Transient thermal error modeling of a ball screw feed system
Authors: Su Dongxu , Li Yang , Zhao Wanhua , Zhang Huijie 
Published: June 2022

Abstract
The thermal behaviors of the ball screw feed system significantly affect the machining accuracy of the machine tool, which is widely valued by users and manufacturers. In the current ball screws thermal error models, the moving heat source at nuts is simplified to a fixed heat source to simplify the calculation, which can decrease the model’s prediction accuracy. A novel thermal error modeling method for the ball screw feed system is proposed by this paper based on the FEM. In the proposed method, the moving heat source at the nut is loaded into the model by changing the grid distribution at different time steps. Meanwhile, a series of thermal experiments are carried out to collect the thermodynamic data with the machine tool’s feed drive system operating at a different speed. And then the multi-objective optimization method is introduced to obtain the heat generated by the nuts and the convection coefficient between the air and the screw. The experimental results show that the thermal error of the ball screw feed system is different when the feed system runs in different intervals, even if the temperature rise at measuring points is the same. In the final, experiments under different conditions are carried out and the proposed model is accurate enough to predict the thermal error of the ball screw feed system.
Case report: A Rare Case Involving the Coexistence of Glioblastoma Multiforme, Arteriovenous Malformation and Intracranial Aneurysm
Authors: Li Chi-Ruei , Shen Chiung-Chyi , Yang Meng-Yin , Cheng Wen-Yu , Chang Cheng-Siu , Chen Se-Yi 
Published: June 2022

Abstract
BackgroundThe coexistence of Glioblastoma Multiforme (GBM) and Arteriovenous Malformation (AVM) is rarely reported in literature. Additionally, there is a paucity of data surrounding the simultaneous presentation of Glioblastoma Multiforme (GBM) with intracranial aneurysms. In the English literature, the concurrence of these three intracranial lesions has never been reported. Regarding our case, we present a case of an Internal Carotid Artery (ICA) aneurysm that occurred in a patient with coexisting GBM and AVM. In addition, we discuss the current literature relating to this unique combination of medical conditions.Case PresentationThe 44-year-old female was presented to our hospital experiencing intermittent headaches and dizziness. Magnetic Resonance Imaging (MRI) revealed a heterogeneously enhanced mass with peri-focal edema at the right temporal lobe. Upon additional computed tomographic angiography study, the presence of AVM nidus at the right temporal-parietal occipital region was noted. Moreover, the image study also demonstrated a right ICA aneurysm. After surgery, histopathological examination demonstrated GBM and AVM in the separately resected lesion specimen. Furthermore, we expected that the ICA aneurysm would reduce in size after removal of the ipsilateral AVM and glioblastoma. Unfortunately, the postoperative digital subtraction angiography revealed only sparse change in the right ICA aneurysm, resulting in the patient still needing to receive intervention involving flow diverter stent placement.ConclusionsIn this case, we discuss the current literature relating to this unique combination of medical conditions. The causality and correlation of this presentation remains uncertain. With regards to our case, the pre-surgical image survey provided us with much support towards making critical decisions. Surgery surrounding the removal of simultaneously coexisting AVM and GBM is always a challenging task due to the potential risk of massive intraoperative bleeding.
Clinical utility of chromosomal microarray analysis and whole exome sequencing in fetuses with oligohydramnios
Authors: Shi# Xiaomei , Ding# Hongke , Li Chen , Liu Ling , Yu LiHua , Zhu Juan , Wu Jing 
Published: June 2022

Abstract
Background: Oligohydramnios is associated to a high rate of pregnancy complications and increased fetal morbidity and mortality. Numerous genetic disorders can present with oligohydramnios. This study aims to evaluate the clinical utility of chromosomal microarray analysis (CMA) and whole exome sequencing (WES) in fetuses with oligohydramnios.Methods: In this retrospective study, 126 fetuses with oligohydramnios at our center from 2018 to 2021 were reviewed. Results of CMA and WES were analyzed.Results: One hundred and twenty-four cases had CMA performed and 32 cases had WES performed. The detection rate of pathogenic/likely pathogenic (P/LP) copy number variants (CNVs) by CMA was 1.6% (2/124). WES revealed P/LP variants in 21.8% (7/32) of fetuses. Six (85.7%, 6/7) fetuses showed an autosomal recessive inheritance pattern. Three (42.9%, 3/7) variants were involved in the renin-angiotensin-aldosterone system (RAAS), which are the known genetic causes of autosomal recessive renal tubular dysgenesis (ARRTD).Conclusion: CMA has low diagnostic utility for oligohydramnios. WES is an efficient approach and should be recommended in cases of oligohydramnios.
Candidate pathophysiology processes in Fragile-X associated neuropsychiatric disorders: An event-related potential study
Authors: Harold Roslyn , Kelleher Bridgette , Novak Keisha , Neo Wei Siong , Stump Tim R , Lee Taylor , Garwood Tessa , Berry-Kravis Elizabeth , Foti Dan 
Published: June 2022

Abstract
BackgroundWomen who carry the premutation of the fragile X mental retardation 1 (FMR1) gene are at high risk for adult-onset major depressive disorder. The current study explores abnormal reward sensitivity (indexed by the reward positivity [RewP]) and error processing (indexed by the error-related negativity [ERN]) as candidates processes of depression risk in this population. Both the RewP and ERN have been linked to depression risk in the general population, but are unexplored within FMR1 premutation carriers (PMC).MethodData were collected from 16 adult PMC women and 16 women with no history of fragile X syndrome, autism, or intellectual disability. Participants completed validated measures of neural sensitivity to rewards (a simple guessing task) and errors (arrow flankers task) while the electroencephalogram was recorded. Cognitive functioning and lifetime history of depression were also assessed.ResultsBoth the ERN and RewP were abnormal among premutation carriers: the topography of the ERN was altered among carriers, and the amplitude of the RewP was reduced among carriers. Both effects were attributable to PMC status, as they remained significant after controlling for differences in verbal comprehension and lifetime history of depression. ConclusionThe FMR1premutation may confer increased risk for depression in part through abnormal neural sensitivity to reward delivery and error commission, which are pathophysiological processes of depression that are shared with the general population. If validated in larger samples, the RewP and ERN may potentially be used as risk markers for depression to aid early intervention and prevention efforts.
Evaluation of some oxidative stress biomarkers in Syrian patients with Chronic Kidney Disease: a case-control study
Authors: Al-torkmany Kassem , Al-zeer Samar 
Published: June 2022

Abstract
Introduction: Oxidative stress is defined as an imbalance between oxidant production and antioxidant defense mechanisms, in favor of oxidant production. Oxidative stress has been implicated in the pathogenesis and development of chronic kidney disease (CKD). Hemodialysis (HD) has also been described to contribute remarkably to oxidative stress in CKD patients. This study aims to evaluate the levels of different oxidative stress biomarkers in Syrian patients with chronic kidney disease (CKD) that also analyzed the effect of HD on redox status.Materials and Methods: Blood samples were collected from CKD (n=30) and HD (n=30) patients and healthy individuals (n=30), and the following markers of oxidative stress were analyzed: malondialdehyde (MDA), advanced oxidation protein product (AOPP), glutathione (GSH), in addition to Hgb, Hct, CRP and Cr.Results: We have found that the levels of MDA, AOPP, CRP, and Cr in CKD and HD groups were significantly higher than in the control group (P=0.0001, P=0.003, P=0.0001, and P=0.0001 respectively). Also, we have found that the levels of GSH, Hgb, and Hct in CKD and HD groups were significantly lower than in the control group (P=0.0001, P=0.0001and P=0.0001, respectively). On the other hand, there were no statistically significant differences (P>0.05) in MDA, AOPP, GSH, and CRP between CKD and HD groups whilst there were statistically significant differences (P<0.05) in Hgb, Hct, and Cr between CKD and HD groups.Conclusions: CKD and HD patients have both higher concentrations of all studied serum oxidative stress biomarkers and decreased concentrations of antioxidants. Also, the results showed that the greatest influence on the development of oxidative stress is the severity of renal failure rather than Hemodialysis treatment.
Comparative study on the nutritional content and physical attributes of giant freshwater prawn (Macrobrachium rosenbergii) and redclaw crayfish (Cherax quadricarinatus) meats
Authors: Tee Zheng-Bin , Ibrahim Saadiah , Teoh Chaiw-Yee 
Published: June 2022

Abstract
Giant freshwater prawn (GFP), Macrobrachium rosenbergii and redclaw crayfish (RC), Cherax quadricarinatus are among the commercial aquaculture species of Malaysia. The tail meat yield and nutritional content are important to both producers and consumers and the present study aimed to determine both aspects for both species. A total of 180 GFP and RC samples consisting of 45 males and females each were obtained, and their physical attributes and proximate composition of tail meat were analysed. Samples were grouped into four categories with triplicates each (n = 3): male GFP, female GFP, male RC, female RC. Individual body weight, tail meat weight, individual body width, and individual body length were measured and the percentage of edible part in relation to body weight was calculated for physical attributes. Moisture, ash, protein, and lipid content of the tail meat for each group were determined for nutritional content. The results indicated that female GFP had significantly (P < 0.05) highest percentage of edible meat (36.15%), and GFP had significantly higher percentage of edible part than RC regardless of the gender. Moreover, both gender and species demonstrated significant effect on the percentage of edible part. On the other hand, male RC had significantly higher moisture (79.17%), ash (1.66%), and lipid (2.98%) contents than male and female GFP, whereas male GFP had significantly highest protein content (20.02%). To sum up, the present study found out that female GFP had the highest tail meat yield, male RC had the highest moisture, ash, and lipid contents, and male GFP had the highest protein content. Besides, different species or gender contributes differently on body growth and development, and that the nutritional content (protein, lipid, ash) of male and female individual is different.
Relationship Among Water, Sediment and Intestinal Microbial Community Structure between Breeding Group and Control Group of Juvenile Huanghe Carp New Strain
Authors: Su Shengyan , Jing Xiaojun , Lu Zeyu , Li Zhixun , Zhou Xiaolin , Li Bing , Tang Yongkai , Zhu Jian , Zhang Chengfeng 
Published: June 2022

Abstract
A total of 1500 fish species in the Yellow River carp breeding group and the control group were reared in the pond and fed with commercial bait three times a day. In the first 2 months of feeding, samples were taken at 4 time points to measure water quality parameters. At the fourth time point, intestinal samples were taken for DNA extraction and purification. PCR amplification, purification and 16S amplicon sequencing were performed. The results showed that except for NO2-N, NO3-N and transparency, the other 11 water quality indexes were closely related to water quality bacterial community. A total of 1,364,230 original tags were obtained for 50 samples including water, sediment and intestines, with an average of 56, 842.9 tags per sample. Finally, 5105 operational taxa (OTUs) were isolated from all samples. OTU mean abundance was highest in water (1228.08±155.87). It was lowest in intestinal tract (717.50±95.31). The Alpha diversity in breeding group was higher than that in control group. There was a clear dividing line between the control group and the selective group. The intestinal microbes first clustered with the water quality microbes, and then clustered with the sediment microbial community. From the perspective of bacterial community structure, whether in the control group or the breeding group, Needle-shaped bacteria were dominant at the 2nd, 3rd and 4th time points of water samples, while Limnohabitans were dominant at the 1st time point. Rhodobacter was dominant in the intestinal tract of both the breeding group and the control group, while Firmicutes were dominant in the bottom mud. The bacteria ranked second and third at the first time point in the water were consistent with those in the bottom mud. In the intestinal tract, the abundance of Chlorooscilla and Firmicutes was different in the breeding group. These results indicated that the breeding group of new strain of Yellow River carp had specific microflora structure.
Predictive value of p16-ki67 double staining of cell block in high-grade cervical intraepithelial neoplasia and grades above it
Authors: Meng Fanfan , Gu Yiqun , Wang Li , Sun Xiaofei 
Published: June 2022

Abstract
Objectives: we performed p16-ki67 immunohistochemical double staining on cervical cytological specimens to shunt patients with high risk human papillomavirus positive(HR-HPV) and cytological diagnosis of atypical squamous sells of undetermined significance(ASC-US), and to explore its expression in cervical intraepithelial neoplasia grade 2 and above (Cin2 +) patients.Methods: Age 21-74 years old woman, who were screened for cervical cancer, tested for HPV and cervical cytology in Haidian District Maternal and Child Health care Hospital from January to December 2021 were selected as the research objects. A subset of paired cervical and vaginal specimens (n = 261) were selected to undergo p16-Ki-67 dual-stain cytology, liquid-based cytology and HR-HPV detection.Results: (1)1) 239 HR-HPV positive patients (239 / 261), of which 80 had histopathological results of Cin2 + and 159 had histopathological results of negative. The sensitivity, specificity, positive predictive value and negative predictive value of Cin2 + detected by HR-HPV were 98.8% (80 / 81), 11.7% (21 / 180), 33.5% (80 / 239) and 95.5% (21 / 22) respectively; Among 261 patients, 80 cases were positive for p16-Ki67 double staining and 181 cases were negative.The sensitivity, specificity, positive predictive value and negative predictive value of patients with Cin2 + were 86.4% (70 / 81), 94.4% (170 / 180), 87.5% (70 / 80) and 93.9% (170 / 181) respectively; Among the 261 patients, 233 were above ASC-US and 28 were negative. The sensitivity, specificity, positive predictive value and negative predictive value of Cin2 + patients were 91.4% (74 / 81), 11.7% (21 / 180), 31.8% (74 / 233) and 75% (21 / 28), respectively; 2)The kappa values of patients with HR-HPV infection, p16-ki67 double staining and TCT diagnosis above ASC-US and histopathological results were 0.068, 0.811 and 0.02 respectively.(2)The positive rates of p16-ki67 double staining in 239 HR-HPV positive patients and 22 negative patients were 33% (79 / 239) and 4.5% (1 / 22), respectively, with significant difference（ χ2=7.703，p=0.006）.(3)The results showed that TCT had statistical significance for patients with Cin2 + detected in histology (P = 0.041). The positive expression of p16-ki67 double staining was a risk factor for Cin2 + patients (P < 0.05).Conclusion: Our study shows that p16-Ki67 double staining has certain sensitivity and specificity, and has high application value for screening Cin2 + population. In particular, it can be used as an important reference index for negative TCT, ASC-US and low grade squamous intraepithelial lesion(LSIL) results.
Estrogen prevents atherosclerosis by promoting macrophage cholesterol crystal metabolism via estrogen receptor alpha (ERα)
Authors: Li Jun , Ding Xue , Zhang Shu rui , Han Feng , Fu Yu , Chao Ying , Ji Tingting , Yu Xichao , Zhang Weiwei , Bi Yunhui , Zhang Yuhan , Chen Qi , Meng Qinghai , Li Yu , Bian Huimin 
Published: June 2022

Abstract
The accumulation of macrophages and cholesterol crystals in atherosclerotic plaques is an important pathological feature of patients with the acute coronary syndrome (ACS). Decreased estrogen levels and estrogen receptors (ERs) lead to an increase in atherosclerosis in postmenopausal women. Whether estrogen prevents atherosclerosis by promoting macrophage cholesterol crystal metabolism via estrogen receptor alpha (ERα) is unknown. The ascending aorta of postmenopausal women decreased the fibrous cap around the plaque, increased the aggregation of CC and macrophages, and reduced the expression of ERα, and transcription factor EB (TFEB). Estrogen supplementation ameliorated atherosclerosis, and reduced CC and macrophages in the cardiac aorta and CC in primary peritoneal macrophages in OVX LDLR−/− mice. Silencing TFEB eliminated the effect of estrogen in enhancing lysosomal function, promoting CC internalization in lysosomes, and promoting reverse cholesterol transport (RCT) through APOA1, but did not affect triglyceride (TG) accumulation or RCT mediated by HDL in macrophages. Inhibition of hormone-sensitive lipase (HSL) decreased the effect of estrogen in increasing RCT through APOA1 and HDL and decreasing TG in macrophages. Inhibition of ERα weakened the effect of estrogen in reducing CC, TG, total cholesterol(TC), free cholesterol(FC), and cholesterol ester (CE) in macrophages. Silencing ERα eliminated the effect of estrogen in increasing lysosomal function and promoting the expression of TFEB, HSL, ABCA1/ABCG1 and inhibiting the expression of NLRP3 and IL-1β in macrophages. Our results provide evidence that estrogen promotes macrophage CC metabolism, which includes increasing the internalization of CC in lysosomes through rescuing the lysosomal biogenesis program by the ERα-TFEB pathway and promoting RCT via ERα-TFEB and HSL pathways to increase the cholesterol efflux.
Development of Cohort-Based Prediction Model for Human Health in Relation to Ecological Aspects
Authors: Pimpale Yogita , Gupta Sachin 
Published: June 2022

Abstract
The ecological aspects such as environmental factors, socio economic constraints and demographic parameters are one of the key aspects to examine the health benefits of human subject and used as ready reference in eco system modelling. Presently, there are various kinds of deadly diseases and disorders who are liable for affecting the human health and impacting the eco framework of whole world. The virus such as Corona, Swine Flu, omicron and others are one of the best examples for the research community to understand the vulnerability of human health in relation to these unpredictable causes. As per report of world health organization every year more than ten million people are affected by such ecological and environmental disbalance. The burden of ecological aspects apparently affecting the working of various organs in human subject. There is a need to understand this ecological model in relation to health of human subjects. In this study, a cohort-based data set of Ecological pollutants and Physiological signals such as ECG and anthropogenic data of human subjects were extracted from Maharashtra from 2015 to 2021. As per neural network-based hazard ratio was calculated and observed to be deplorable among unhealthy and health categories of human subjects. The accumulative eco system is responsible for overburden to organs of living beings and policy makers must focus on the facts of study for modern management framework designs.
CMOS-Compatible Ising Machines built using Bistable Latches Coupled through Ferroelectric Transistor Arrays
Authors: Mallick Antik , Zhao Zijian , Bashar Mohammad Khairul , Alam Shamiul , Islam Md Mazharul , Xiao Yi , Xu Yixin , Aziz Ahmedullah , Narayanan Vijaykrishnan , Ni Kai , Shukla Nikhil 
Published: June 2022

Abstract
Realizing compact and scalable Ising machines that are compatible with CMOS-process technology is crucial to the effectiveness and practicality of using such hardware platforms for accelerating computationally intractable problems. Besides the need for realizing compact Ising spins, the implementation of the coupling network, which describes the spin interaction, is also a potential bottleneck in the scalability of such platforms. Therefore, in this work, we propose an Ising machine platform that exploits the novel behavior of compact bi-stable CMOS-latches (cross-coupled inverters) as classical Ising spins interacting through highly scalable and CMOS-process compatible ferroelectric-HfO2-based Ferroelectric FETs (FeFETs) which act as coupling elements. We experimentally demonstrate the prototype building blocks of this system, and evaluate the behavior of the scaled system using simulations. We project that the proposed architecture can compute Ising solutions with an efficiency of ~1.04 x 108 solutions/W/second. Our work not only provides a pathway to realizing CMOS-compatible designs but also to overcoming their scaling challenges.