bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-32 promotes MYC-driven prostate cancer
Authors: Scaravilli Mauro , Koivukoski Sonja , Gillen Andrew , Bouazza Aya , Ruusuvuori Pekka , Visakorpi Tapio , Latonen Leena 
Published: March 2022

AbstractmiR-32 is an androgen receptor (AR)-regulated microRNA, expression of which is increased in castration-resistant prostate cancer (PC). We have previously shown that overexpression of miR-32 in the prostate of transgenic mice potentiates proliferation in prostate epithelium. Here, we set out to determine whether increased expression of miR-32 influences growth or phenotype in prostate adenocarcinoma in vivo. We studied transgenic mice expressing MYC oncogene (hiMYC mice) to induce tumorigenesis in the mouse prostate and discovered that transgenic overexpression of miR-32 resulted in increased tumor burden as well as a more aggressive tumor phenotype in this model. Elevated expression of miR-32 increased proliferation as assessed by Ki-67 immunohistochemistry, increased nuclear density, and higher mitotic index in the tumors. By gene expression analysis of the tumorous prostate tissue, we confirmed earlier findings that miR-32 expression regulates prostate secretome by modulating expression levels of several PC-related target genes such asSpink1,Spink5, andMsmb. Further, we identifiedPdk4as a tumor-associated miR-32 target in the mouse prostate. Expression analysis ofPDK4in human PC reveals an inverse correlation with miR-32 expression and Gleason score, a decrease in castration-resistant and metastatic tumors compared to untreated primary PC, and an association of lowPDK4expression with a shorter recurrence-free survival of patients. Although decreased PDK4 expression induces the higher metabolic activity of PC cells, induced expression of PDK4 reduces both mitotic respiration and glycolysis rates as well as inhibits cell growth. In conclusion, we show that miR-32 promotes MYC-induced prostate adenocarcinoma and identifies PDK4 as a PC-relevant metabolic target of miR-32-3p.
Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm
Authors: Jia Yiting , Zhang Lu , Liu Ziyi , Mao Chenfeng , Ma Zihan , Li Wenqiang , Yu Fang , Wang Yingbao , Huang Yaqian , Zhang Weizhen , Zheng Jingang , Wang Xian , Xu Qingbo , Zhang Jian , Feng Wei , Yun Caihong , Liu Chuanju , Sun Jinpeng , Fu Yi , Cui Qinghua , Kong Wei 
Published: March 2022

AbstractAbdominal aortic aneurysm (AAA) is a lethal cardiovascular disease, and there is no proven drug treatment for this condition. In this study, by using the Connectivity Map (CMap) approach, we explored naringenin, a naturally occurring citrus flavonoid, as a putative agent for inhibiting AAA. We then validated the prediction with two independent mouse models of AAA, calcium phosphate (CaPO4)-induced C57BL/6J mice and angiotensin II-infused ApoE−/− mice. Naringenin effectively blocked the formation of AAAs and the progression of established AAAs. Transcription factor EB (TFEB) is the master regulator of lysosome biogenesis. Intriguingly, the protective role of naringenin on AAA was abolished by macrophage-specific TFEB depletion in mice. Unbiased interactomics, combined with isothermal titration calorimetry (ITC) and cellular thermal shift assays (CETSAs), further revealed that naringenin is directly bound to 14-3-3 epsilon blocked the TFEB-14-3-3 epsilon interaction, and therefore promoted TFEB nuclear translocation and activation. On one hand, naringenin activated lysosome-dependent inhibition of the NLRP3 inflammasome and repressed aneurysmal inflammation. On the other hand, naringenin induced TFEB-dependent transcriptional activation of GATA3, IRF4, and STAT6 and therefore promoted reparative M2 macrophage polarization. In summary, naturally derived naringenin or macrophage TFEB activation shows promising efficacy for the treatment of AAA.
Possibility for strong northern hemisphere high-latitude cooling under negative emissions
Authors: Schwinger Jörg , Asaadi Ali , Goris Nadine , Lee Hanna 
Published: March 2022

AbstractIt is well established that a collapse or strong reduction of the Atlantic meridional overturning circulation (AMOC) would substantially cool the northern high latitudes. Here we show that there is a possibility that such cooling could be amplified under deliberate CO2 removal and result in a temporary undershoot of a targeted temperature level. We find this behaviour in Earth system models that show a strong AMOC decline in response to anthropogenic forcing. Idealized simulations of CO2 removal with one of these models indicate that the timing of negative emissions relative to AMOC decline and recovery is key in setting the strength of the temporary cooling. We show that the pronounced temperature-fluctuations at high northern latitudes found in these simulations would entail considerable consequences for sea-ice and permafrost extent as well as for high latitude ecosystems.
Loss of MIG-6 results in endometrial progesterone resistance via ERBB2
Authors: Yoo Jung-Yoon , Kim Tae Hoon , Shin Jung-Ho , Marquardt Ryan M. , Müller Ulrich , Fazleabas Asgerally T. , Young Steven L. , Lessey Bruce A. , Yoon Ho-Geun , Jeong Jae-Wook 
Published: March 2022

AbstractFemale subfertility is highly associated with endometriosis. Endometrial progesterone resistance is suggested as a crucial element in the development of endometrial diseases. We report that MIG-6 is downregulated in the endometrium of infertile women with endometriosis and in a non-human primate model of endometriosis. We find ERBB2 overexpression in the endometrium of uterine-specific Mig-6 knockout mice (Pgrcre/+Mig-6f/f; Mig-6d/d). To investigate the effect of ERBB2 targeting on endometrial progesterone resistance, fertility, and endometriosis, we introduce Erbb2 ablation in Mig-6d/d mice (Mig-6d/dErbb2d/d mice). The additional knockout of Erbb2 rescues all phenotypes seen in Mig-6d/d mice. Transcriptomic analysis shows that genes differentially expressed in Mig-6d/d mice revert to their normal expression in Mig-6d/dErbb2d/d mice. Together, our results demonstrate that ERBB2 overexpression in endometrium with MIG-6 deficiency causes endometrial progesterone resistance and a nonreceptive endometrium in endometriosis-related infertility, and ERBB2 targeting reverses these effects.
Exchange-biased topological transverse thermoelectric effects in a Kagome ferrimagnet
Authors: Zhang Heda , Koo Jahyun , Xu Chunqiang , Sretenovic Milos , Yan Binghai , Ke Xianglin 
Published: March 2022

AbstractKagome metal TbMn6Sn6 was recently discovered to be a ferrimagnetic topological Dirac material by scanning tunneling microscopy/spectroscopy measurements. Here, we report the observation of large anomalous Nernst effect and anomalous thermal Hall effect in this compound. The anomalous transverse transport is consistent with the Berry curvature contribution from the massive Dirac gaps in the 3D momentum space as demonstrated by our first-principles calculations. Furthermore, the transverse thermoelectric transport exhibits asymmetry with respect to the applied magnetic field, i.e., an exchange-bias behavior. Together, these features place TbMn6Sn6 as a promising system for the outstanding thermoelectric performance based on anomalous Nernst effect.
Different expression of circulating microRNA profile and plasma SP-D in Tibetan COPD patients
Authors: Shi Xue-feng , He Xiang , Sun Ze-rui , Wang Jian-xiang , Gu Yu-hai , Xie You-bang , Duo Jie 
Published: March 2022

AbstractCOPD is the fourth leading cause of mortality, and is predicted to be the third leading cause of death worldwide by 2020. But few studies on Tibetan COPD of China. This study identifies distinctive miRNA signatures in Tibetan COPD patients from Tibetan healthy subjects that could serve as diagnostic biomarkers or describe differential molecular mechanisms with potential therapeutic implications. In this study, a total of 210 differentially expressed miRNAs were screened. Analysis of the functions of target genes of differentially expressed miRNAs via GO enrichment analysis revealed that they mainly influenced guanyl-nucleotide exchange factor activity, cell morphogenesis and the positive regulation of GTPase activity. KEGG pathway enrichment analysis showed that these target genes were mainly enriched in signaling by NGF, Axon guidance, developmental biology, ubiquitin mediated proteolysis, and PDGF signaling pathways. MiR-106-5p and miR-486-5p expression was validated in the complete cohort. Age, plasma miR-106-5p, miR-486-5p, SP-D protein levels, and SP-D mRNA level were also determined to be correlated with FEV1%Pred, and may as the risk factors of Tibetan COPD. The combination of plasma miR-106-5p, miR-486-5p and SP-D mRNA expression may be the best model to assist the diagnosis of Tibetan COPD.
Liver injury in non-alcoholic fatty liver disease is associated with urea cycle enzyme dysregulation
Authors: Gallego-Durán Rocío , Ampuero Javier , Pastor-Ramírez Helena , Álvarez-Amor Leticia , del Campo Jose Antonio , Maya-Miles Douglas , Montero-Vallejo Rocío , Cárdenas-García Antonio , Pareja Mª Jesús , Gato-Zambrano Sheila , Millán Raquel , del Carmen Rico María , Luque-Sierra Amparo , Gil-Gómez Antonio , Rojas Ángela , Muñoz-Hernández Rocío , García-Lozano María , Aller Rocío , Andrade Raúl J. , García-Monzón Carmelo , Andreola Fausto , Martín Francisco , Jalan Rajiv , Romero-Gómez Manuel 
Published: March 2022

AbstractThe main aim was to evaluate changes in urea cycle enzymes in NAFLD patients and in two preclinical animal models mimicking this entity. Seventeen liver specimens from NAFLD patients were included for immunohistochemistry and gene expression analyses. Three-hundred-and-eighty-two biopsy-proven NAFLD patients were genotyped for rs1047891, a functional variant located in carbamoyl phosphate synthetase-1 (CPS1) gene. Two preclinical models were employed to analyse CPS1 by immunohistochemistry, a choline deficient high-fat diet model (CDA-HFD) and a high fat diet LDLr knockout model (LDLr −/−). A significant downregulation in mRNA was observed in CPS1 and ornithine transcarbamylase (OTC1) in simple steatosis and NASH-fibrosis patients versus controls. Further, age, obesity (BMI > 30 kg/m2), diabetes mellitus and ALT were
found to be risk factors whereas A-allele from CPS1 was a protective factor from liver fibrosis. CPS1 hepatic expression was diminished in parallel with the increase of fibrosis, and its levels reverted up to normality after changing diet in CDA-HFD mice. In conclusion, liver fibrosis and steatosis were associated with a reduction in both gene and protein expression patterns of mitochondrial urea cycle enzymes. A-allele from a variant on CPS1 may protect from fibrosis development. CPS1 expression is restored in a preclinical model when the main trigger of the liver damage disappears.
Quantum teleportation with one classical bit
Authors: Parakh Abhishek 
Published: March 2022

AbstractQuantum teleportation allows one to transmit an arbitrary qubit from point A to point B using a pair of (pre-shared) entangled qubits and classical bits of information. The conventional protocol for teleportation uses two bits of classical information and assumes that the sender has access to only one copy of the arbitrary qubit to be sent. Here, we ask whether we can do better than two bits of classical information if the sender has access to multiple copies of the qubit to be teleported. We place no restrictions on the qubit states. Consequently, we propose a modified quantum teleportation protocol that allows Alice to reset the state of the entangled pair to its initial state using only local operations. As a result, the proposed teleportation protocol requires the transmission of only one classical bit with a probability greater than one-half. This has implications for efficient quantum communications and the security of quantum cryptographic protocols based on quantum entanglement.
Synthetic correlated diffusion imaging hyperintensity delineates clinically significant prostate cancer
Authors: Wong Alexander , Gunraj Hayden , Sivan Vignesh , Haider Masoom A. 
Published: March 2022

AbstractProstate cancer (PCa) is the second most common cancer in men worldwide and the most frequently diagnosed cancer among men in more developed countries. The prognosis of PCa is excellent if detected at an early stage, making early screening crucial for detection and treatment. In recent years, a new form of diffusion magnetic resonance imaging called correlated diffusion imaging (CDI) was introduced, and preliminary results show promise as a screening tool for PCa. In the largest study of its kind, we investigate the relationship between PCa presence and a new variant of CDI we term synthetic correlated diffusion imaging (CDI$$^s$$

s

), as well as its performance for PCa delineation compared to current standard MRI techniques [T2-weighted (T2w) imaging, diffusion-weighted imaging (DWI), and dynamic contrast-enhanced (DCE) imaging] across a cohort of 200 patient cases. Statistical analyses reveal that hyperintensity in CDI$$^s$$

s

is a strong indicator of PCa presence and achieves strong delineation of clinically significant cancerous tissue compared to T2w, DWI, and DCE. These results suggest that CDI$$^s$$

s

hyperintensity may be a powerful biomarker for the presence of PCa, and may have a clinical impact as a diagnostic aid for improving PCa screening.
A sensitivity study of rising compound coastal inundation over large flood plains in a changing climate
Authors: Peter Sheng Y. , Paramygin Vladimir A. , Yang Kun , Rivera-Nieves Adail A. 
Published: March 2022

AbstractCoastal flood hazards and damage to coastal communities are increasing steeply and nonlinearly due to the compound impact of intensifying tropical cyclones (TCs) and accelerating sea-level rise (SLR). We expand the probabilistic coastal flood hazard analysis framework to facilitate coastal adaptation by simulating the compound impact of predicted intensifying TCs and rising sea levels in the twenty-first century. We compared the characteristics of landfalling TCs in Florida (FL) and southwest Florida (SWFL) for the late twentieth and twenty-first centuries predicted by several climate models and downscaling models. TCs predicted by four climate models, one without downscaling and three with downscaling, were used by a coupled surge-wave model to predict the future flood hazard due to compound effects of TCs and SLR over a large SWFL coastal flood plain. By 2100, the coastal inundation metrics of the 1% annual chance coastal flood could become almost 3–7 folds of their current values, depending on the climate and downscaling models, Representative Concentration Pathway scenarios, Atlantic Multi-decadal Oscillation phases, TCs, SLR, precipitation, and how TCs and SLR are incorporated. By 2100, the current 1% (100-year) inundation event could become a 3-year event, and the 0.2% (500-year) inundation event could become a 5-year event.
SARS-CoV-2 enzyme-linked immunosorbent assays as proxies for plaque reduction neutralisation tests
Authors: Kay Grant A. , Owen Sophie I. , Giorgi Emanuele , Clark David J. , Williams Christopher T. , Menzies Stefanie , Cuevas Luis E. , Davies Benedict M. O. , Eckersley Nicholas M. , Hughes Grant L. , Kirwan Daniela E. , Krishna Sanjeev , Patterson Edward I. , Planche Tim , Staines Henry M. , Adams Emily R. 
Published: March 2022

AbstractSevere acute respiratory coronavirus 2 (SARS-CoV-2) has spread globally since its emergence in 2019. Most SARS-CoV-2 infections generate immune responses leading to rising levels of immunoglobulins (Ig) M, A and G which can be detected using diagnostic tests including enzyme-linked immunosorbent assays (ELISA). Whilst implying previous SARS-CoV-2 infection, the detection of Ig by ELISA does not guarantee the presence of neutralising antibodies (NAb) that can prevent the virus infecting cells. Plaque reduction neutralisation tests (PRNT) detect NAb, but are not amenable to mass testing as they take several days and require use of SARS-CoV-2 in high biocontainment laboratories. We evaluated the ability of IgG and IgM ELISAs targeting SARS-CoV-2 spike subunit 1 receptor binding domain (S1-RBD), and spike subunit 2 (S2) and nucleocapsid protein (NP), at predicting the presence and magnitude of NAb determined by PRNT. IgG S2 + NP ELISA was 96.8% [95% CI 83.8–99.9] sensitive and 88.9% [95% CI 51.8–99.7] specific at predicting the presence of NAbs (PRNT80 > 1:40). IgG and IgM S1-RBD ELISAs correlated with PRNT titre, with higher ELISA results increasing the likelihood of a robust neutralising response. The IgM S1-RBD assay can be used as a rapid, high throughput test to approximate the magnitude of NAb titre.
Magnetisation switching dynamics induced by combination of spin transfer torque and spin orbit torque
Authors: Meo Andrea , Chureemart Jessada , Chantrell Roy W. , Chureemart Phanwadee 
Published: March 2022

AbstractWe present a theoretical investigation of the magnetisation reversal process in CoFeB-based magnetic tunnel junctions (MTJs). We perform atomistic spin simulations of magnetisation dynamics induced by combination of spin orbit torque (SOT) and spin transfer torque (STT). Within the model the effect of SOT is introduced as a Slonczewski formalism, whereas the effect of STT is included via a spin accumulation model. We investigate a system of CoFeB/MgO/CoFeB coupled with a heavy metal layer where the charge current is injected into the plane of the heavy metal meanwhile the other charge current flows perpendicular into the MTJ structure. Our results reveal that SOT can assist the precessional switching induced by spin polarised current within a certain range of injected current densities yielding an efficient and fast reversal on the sub-nanosecond timescale. The combination of STT and SOT gives a promising pathway to improve high performance CoFeB-based devices with high speed and low power consumption.
Diversity of prokaryotic microorganisms in alkaline saline soil of the Qarhan Salt Lake area in the Qinghai–Tibet Plateau
Authors: Wang Yaqiong , Bao Guoyuan 
Published: March 2022

AbstractThe composition of microbial communities varies considerably across ecological environments, particularly in extreme environments, where unique microorganisms are typically used as the indicators of environmental conditions. However, the ecological reasons for the differences in microbial communities remain largely unknown. Herein, we analyzed taxonomic and functional community profiles via high-throughput sequencing to determine the alkaline saline soil bacterial and archaeal communities in the Qarhan Salt Lake area in the Qinghai–Tibet Plateau. The results showed that Betaproteobacteria (Proteobacteria) and Halobacteria (Euryarchaeota) were the most abundant in the soils of this area, which are common in high salinity environments. Accordingly, microbes that can adapt to local extremes typically have unique metabolic pathways and functions, such as chemoheterotrophy, aerobic chemoheterotrophy, nitrogen fixation, ureolysis, nitrate reduction, fermentation, dark hydrogen oxidation, and methanogenesis. Methanogenesis pathways include hydrogenotrophic methanogenesis, CO2 reduction with H2, and formate methanogenesis. Thus, prokaryotic microorganisms in high salinity environments are indispensable in nitrogen and carbon cycling via particular metabolic pathways.
The effect of a variable focal spot size on the contrast channels retrieved in edge-illumination X-ray phase contrast imaging
Authors: Astolfo A. , Buchanan I. , Partridge T. , Kallon G. K. , Hagen C. K. , Munro P. R. T. , Endrizzi M. , Bate D. , Olivo A. 
Published: March 2022

AbstractMulti-modal X-ray imaging allows the extraction of phase and dark-field (or “Ultra-small Angle Scatter”) images alongside conventional attenuation ones. Recently, scan-based systems using conventional sources that can simultaneously output the above three images on relatively large-size objects have been developed by various groups. One limitation is the need for some degree of spatial coherence, achieved either through the use of microfocal sources, or by placing an absorption grating in front of an extended source. Both these solutions limit the amount of flux available for imaging, with the latter also leading to a more complex setup with additional alignment requirements. Edge-illumination partly overcomes this as it was proven to work with focal spots of up to 100 micron. While high-flux, 100 micron focal spot sources do exist, their comparatively large footprint and high cost can be obstacles to widespread translation. A simple solution consists in placing a single slit in front of a large focal spot source. We used a tunable slit to study the system performance at various effective focal spot sizes, by extracting transmission, phase and dark-field images of the same specimens for a range of slit widths. We show that consistent, repeatable results are obtained for varying X-ray statistics and effective focal spot sizes. As the slit width is increased, the expected reduction in the raw differential phase peaks is observed, compensated for in the retrieval process by a broadened sensitivity function. This leads to the same values being correctly retrieved, but with a slightly larger error bar i.e. a reduction in phase sensitivity. Concurrently, a slight increase in the dark-field signal is also observed.
The role of the AC component in human perception of AC–DC hybrid electric fields
Authors: Jankowiak Kathrin , Kaifie Andrea , Krampert Thomas , Kraus Thomas , Kursawe Michael 
Published: March 2022

AbstractElectric energy is essential to today’s society. To cope with global higher demand while minimizing land use, efficient high voltage direct current (HVDC) power lines are planned to be mounted on existing alternating current (AC) structures leading to electric fields (EFs) from both AC and DC transmission lines in hybrid configurations. Due to the close proximity to residential areas, the investigation of human hybrid EF perception and underlying mechanisms will be useful to project permitting. To specify the influence of the AC component on the whole-body detection thresholds of hybrid EFs and to explore the lower bound of human hybrid EF perception, 51 participants with an EF detection ability above average were exposed in a double-blind laboratory study. A psychophysical method based on the signal detection theory was used. Very low EF strength combinations, e.g. 1 kV/m AC combined with 1 kV/m DC, were reliably perceived by at least one participant. Detection thresholds were significantly lower with increased AC EF strengths, underlining the key role of the AC component in the human perception of hybrid EFs. Findings will contribute to the assessment of public reaction to the perception of EFs around hybrid overhead power lines and to their optimal designs.