bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soil fungal communities affect the chemical quality of flue-cured tobacco leaves in Bijie, Southwest China
Authors: Wang Mei , Zhang Long , He Yi , Huang Lukuan , Liu Lei , Chen Dan , Shan Anqi , Feng Ying , Yang Xiaoe 
Published: February 2022

AbstractSoil microorganisms could affect the quality of tobacco leaves, however, little is known about the association of tobacco chemical components and soil fungal communities. In the present study, the relationship between soil fungi and tobacco quality based on chemical components in Bijie was investigated. The results showed that the total harmony scores (THS) of the analyzed tobacco leaves ranged from 46.55 ± 3.5 to 91.55 ± 2.25. Analyses of chemical components revealed that high contents of nicotine (≥ 1.06%) and sugar (total sugar: ≥ 22.96%, reducing sugar: ≥ 19.62%), as well as low potassium level (≤ 2.68%) were the main factors limiting the quality of flue-cured tobacco leaves. Pearson correlation analysis indicated that soil nitrate, available potassium/phosphorous, and organic matter significantly correlated with tobacco nicotine, potassium, and chloride levels (p < 0.05). Besides, the analysis of alpha- and beta-diversity of soil fungal communities implied that fungal structure rather than the richness affected the chemical quality of tobacco. In detail, the relative abundance of Humicola olivacea species in soils was positively correlated with the THS of tobaccos (r = 0.52, p < 0.05). Moreover, the species including Mortierella alpina, Mortierella hyalina, Tausonia pullulan, and Humicola olivacea were negatively correlated with tobacco sugar (r ≤  − 0.45, p < 0.05) while, Codinaea acaciae and Saitozyma podzolica species were negatively correlated with tobacco nicotine (r ≤  − 0.51, p < 0.05). The present study provides a preliminary basis for utilizing fungal species in soils to improve the chemical quality of tobacco in the studied area.
Classification of ischemia from myocardial polar maps in 15O–H2O cardiac perfusion imaging using a convolutional neural network
Authors: Teuho Jarmo , Schultz Jussi , Klén Riku , Knuuti Juhani , Saraste Antti , Ono Naoaki , Kanaya Shigehiko 
Published: February 2022

AbstractWe implemented a two-dimensional convolutional neural network (CNN) for classification of polar maps extracted from Carimas (Turku PET Centre, Finland) software used for myocardial perfusion analysis. 138 polar maps from 15O–H2O stress perfusion study in JPEG format from patients classified as ischemic or non-ischemic based on finding obstructive coronary artery disease (CAD) on invasive coronary artery angiography were used. The CNN was evaluated against the clinical interpretation. The classification accuracy was evaluated with: accuracy (ACC), area under the receiver operating characteristic curve (AUC), F1 score (F1S), sensitivity (SEN), specificity (SPE) and precision (PRE). The CNN had a median ACC of 0.8261, AUC of 0.8058, F1S of 0.7647, SEN of 0.6500, SPE of 0.9615 and PRE of 0.9286. In comparison, clinical interpretation had ACC of 0.8696, AUC of 0.8558, F1S of 0.8333, SEN of 0.7500, SPE of 0.9615 and PRE of 0.9375. The CNN classified only 2 cases differently than the clinical interpretation. The clinical interpretation and CNN had similar accuracy in classifying false positives and true negatives. Classification of ischemia is feasible in 15O–H2O stress perfusion imaging using JPEG polar maps alone with a custom CNN and may be useful for the detection of obstructive CAD.
Investigation of discordant SARS-CoV-2 RT-PCR results using minimally processed saliva
Authors: White Dawn , Gu Jimmy , Steinberg Catherine-Jean , Yamamura Deborah , Salena Bruno J. , Balion Cynthia , Filipe Carlos D. M. , Capretta Alfredo , Li Yingfu , Brennan John D. 
Published: February 2022

AbstractSaliva is an attractive sample for coronavirus disease 2019 testing due its ease of collection and amenability to detect viral RNA with minimal processing. Using a direct-to-RT-PCR method with saliva self-collected from confirmed COVID-19 positive volunteers, we observed 32% false negative results. Confirmed negative and healthy volunteer samples spiked with 106 genome copies/mL of heat-inactivated severe acute respiratory syndrome coronavirus 2 showed false negative results of 10% and 13%, respectively. Additional sample heating or dilution of the false negative samples conferred only modest improvements. These results highlight the potential to significantly underdiagnose COVID-19 infections when testing directly from minimally processed heterogeneous saliva samples.
Central Mongolian lake sediments reveal new insights on climate change and equestrian empires in the Eastern Steppes
Authors: Struck Julian , Bliedtner Marcel , Strobel Paul , Taylor William , Biskop Sophie , Plessen Birgit , Klaes Björn , Bittner Lucas , Jamsranjav Bayarsaikhan , Salazar Gary , Szidat Sönke , Brenning Alexander , Bazarradnaa Enkhtuya , Glaser Bruno , Zech Michael , Zech Roland 
Published: February 2022

AbstractThe repeated expansion of East Asian steppe cultures was a key driver of Eurasian history, forging new social, economic, and biological links across the continent. Climate has been suggested as important driver of these poorly understood cultural expansions, but paleoclimate records from the Mongolian Plateau often suffer from poor age control or ambiguous proxy interpretation. Here, we use a combination of geochemical analyses and comprehensive radiocarbon dating to establish the first robust and detailed record of paleohydrological conditions for Lake Telmen, Mongolia, covering the past ~ 4000 years. Our record shows that humid conditions coincided with solar minima, and hydrological modeling confirms the high sensitivity of the lake to paleoclimate changes. Careful comparisons with archaeological and historical records suggest that in the vast semi-arid grasslands of eastern Eurasia, solar minima led to reduced temperatures, less evaporation, and high biomass production, expanding the power base for pastoral economies and horse cavalry. Our findings suggest a crucial link between temperature dynamics in the Eastern Steppe and key social developments, such as the emergence of pastoral empires, and fuel concerns that global warming enhances water scarcity in the semi-arid regions of interior Eurasia.
Evaluation of white sandstone mechanical behaviour and the energy evolution of prepeak unloading damage
Authors: Liang Zhonghao , Yu Zhuoqun , Guo Longren , Huang Sa , Qin Nan , Wen Zhijie 
Published: February 2022

AbstractDeep high-stress roadway excavation under unloading disturbance inevitably leads to damage deterioration of the surrounding rock, which poses a serious threat to its stability. To explore the energy characteristics of white sandstone damaged by peak front unloading, uniaxial compression tests were conducted on damaged rock samples. The results show that the peak strength and modulus of elasticity of the rock sample gradually decrease with increasing damage degrees. The external work input energy, releasable elastic strain energy and dissipation energy all decreased with increasing damage. Damage evolution curves and equations of the rock samples were obtained based on the damage instantiation model established by the principle of energy dissipation and release. The effects of unloading damage on the fracture characteristics of the rock samples were analysed from both macro and microscopic viewpoints, and the results showed that a micro fracture in the rock is transformed from brittle–ductile damage, while macroscopic damage occurs in the form of a "shear"-"splitting"-"mixed shear-splitting" damage process. This paper has certain research and reference value for understanding the damage evolution characteristics of rocks with peak front unloading damage.
MqsR toxin as a biotechnological tool for plant pathogen bacterial control
Authors: de Souza-Neto Reinaldo Rodrigues , Carvalho Isis Gabriela Barbosa , Martins Paula Maria Moreira , Picchi Simone Cristina , Tomaz Juarez Pires , Caserta Raquel , Takita Marco Aurélio , de Souza Alessandra Alves 
Published: February 2022

AbstractType II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.
Dynamic personalized risk prediction in chronic heart failure patients: a longitudinal, clinical investigation of 92 biomarkers (Bio-SHiFT study)
Authors: Klimczak-Tomaniak Dominika , de Bakker Marie , Bouwens Elke , Akkerhuis K. Martijn , Baart Sara , Rizopoulos Dimitris , Mouthaan Henk , van Ramshorst Jan , Germans Tjeerd , Constantinescu Alina , Manintveld Olivier , Umans Victor , Boersma Eric , Kardys Isabella 
Published: February 2022

AbstractThe aim of our observational study was to derive a small set out of 92 repeatedly measured biomarkers with optimal predictive capacity for adverse clinical events in heart failure, which could be used for dynamic, individual risk assessment in clinical practice. In 250 chronic HFrEF (CHF) patients, we collected trimonthly blood samples during a median of 2.2 years. We selected 537 samples for repeated measurement of 92 biomarkers with the Cardiovascular Panel III (Olink Proteomics AB). We applied Least Absolute Shrinkage and Selection Operator (LASSO) penalization to select the optimal set of predictors of the primary endpoint (PE). The association between repeatedly measured levels of selected biomarkers and the PE was evaluated by multivariable joint models (mvJM) with stratified fivefold cross validation of the area under the curve (cvAUC). The PE occurred in 66(27%) patients. The optimal set of biomarkers selected by LASSO included 9 proteins: NT-proBNP, ST2, vWF, FABP4, IGFBP-1, PAI-1, PON-3, transferrin receptor protein-1, and chitotriosidase-1, that yielded a cvAUC of 0.88, outperforming the discriminative ability of models consisting of standard biomarkers (NT-proBNP, hs-TnT, eGFR clinically adjusted) − 0.82 and performing equally well as an extended literature-based set of acknowledged biomarkers (NT-proBNP, hs-TnT, hs-CRP, GDF-15, ST2, PAI-1, Galectin 3) − 0.88. Nine out of 92 serially measured circulating proteins provided a multivariable model for adverse clinical events in CHF patients with high discriminative ability. These proteins reflect wall stress, remodelling, endothelial dysfunction, iron deficiency, haemostasis/fibrinolysis and innate immunity activation. A panel containing these proteins could contribute to dynamic, personalized risk assessment.Clinical Trial Registration: 10/05/2013 https://clinicaltrials.gov/ct2/show/NCT01851538?term=nCT01851538&draw=2&rank=1.
P2X4 deficiency reduces atherosclerosis and plaque inflammation in mice
Authors: Peikert Alexander , König Sebastian , Suchanek Dymphie , Rofa Karlos , Schäfer Ibrahim , Dimanski Daniel , Karnbrock Lorenz , Bulatova Kseniya , Engelmann Juliane , Hoppe Natalie , Wadle Carolin , Heidt Timo , Albrecht Philipp , von Garlen Sunaina , Härdtner Carmen , Hilgendorf Ingo , Wolf Dennis , von zur Mühlen Constantin , Bode Christoph , Zirlik Andreas , Duerschmied Daniel , Merz Julian , Stachon Peter 
Published: February 2022

AbstractExtracellular adenosine-5′-triphosphate (ATP) acts as an import signaling molecule mediating inflammation via purinergic P2 receptors. ATP binds to the purinergic receptor P2X4 and promotes inflammation via increased expression of pro-inflammatory cytokines. Because of the central role of inflammation, we assumed a functional contribution of the ATP-P2X4-axis in atherosclerosis. Expression of P2X4 was increased in atherosclerotic aortic arches from low-density lipoprotein receptor-deficient mice being fed a high cholesterol diet as assessed by real-time polymerase chain reaction and immunohistochemistry. To investigate the functional role of P2X4 in atherosclerosis, P2X4-deficient mice were crossed with low-density lipoprotein receptor-deficient mice and fed high cholesterol diet. After 16 weeks, P2X4-deficient mice developed smaller atherosclerotic lesions compared to P2X4-competent mice. Furthermore, intravital microscopy showed reduced ATP-induced leukocyte rolling at the vessel wall in P2X4-deficient mice. Mechanistically, we found a reduced RNA expression of CC chemokine ligand 2 (CCL-2), C-X-C motif chemokine-1 (CXCL-1), C-X-C motif chemokine-2 (CXCL-2), Interleukin-6 (IL-6) and tumor necrosis factor α (TNFα) as well as a decreased nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3)-inflammasome priming in atherosclerotic plaques from P2X4-deficient mice. Moreover, bone marrow derived macrophages isolated from P2X4-deficient mice revealed a reduced ATP-mediated release of CCL-2, CC chemokine ligand 5 (CCL-5), Interleukin-1β (IL-1β) and IL-6. Additionally, P2X4-deficient mice shared a lower proportion of pro-inflammatory Ly6Chigh monocytes and a higher proportion of anti-inflammatory Ly6Clow monocytes, and expressend less endothelial VCAM-1. Finally, increased P2X4 expression in human atherosclerotic lesions from carotid endarterectomy was found, indicating the importance of potential implementations of this study’s findings for human atherosclerosis. Collectively, P2X4 deficiency reduced experimental atherosclerosis, plaque inflammation and inflammasome priming, pointing to P2X4 as a potential therapeutic target in the fight against atherosclerosis.
Experimental study on fracture characteristics of rock-like material with prefabricated cracks under compression shear
Authors: Yuan Shuang-Xi , Jiang Tong , Lei Jia-Hua , Cui Cheng-Hao 
Published: February 2022

AbstractIn order to understand the effects of different patterns of prefabricated fractures and grain size compositions on the fracture characteristics, acoustic emission characteristics and mechanical properties of the rock masses. We conducted compression shear experiments on square rock masses with different modes of prefabricated fractures and different grain size compositions. The experimental results showed that five fracture patterns were produced in specimens with different fracture patterns. The first fracture specimen, the fourth fracture specimen and the fifth fracture specimen were all brittle fractures. The other four specimens were not brittle fractures. The fracture patterns, fracture processes and mechanical characteristics of the different fracture pattern rock masses were revealed. The lowest peak shear stresses were found in specimens consisting of two grain size ranges and the highest peak shear stresses were found in specimens consisting of three grain size ranges. The highest shear displacements corresponding to the peak shear stresses were found in the specimens consisting of three particle size ranges. The effect of different grain size compositions on the peak shear stress and its corresponding shear displacement of the rock mass was revealed. Specimens consisting of one grain size range produced significant fracture and acoustic emission prior to the peak shear stress. The acoustic emission was jumped after the main fracture was formed. The specimen consisting of two grain size ranges produced fractures and strong acoustic emission characteristics after the peak shear stress. Thereafter, fracture reappeared and the acoustic emission signature increased again. As the specimen entered the residual strength phase, the acoustic emission was jumpy. Specimens consisting of three grain size ranges were brittle fractures with weak acoustic emission characteristics after the main fracture has formed. The cumulative energy of shear acoustic emission was the highest for a rock mass consisting of three grain size ranges. The rock mass consisting of three grain size ranges was also the strongest and most difficult to fracture because the grains were more fully embedded in each other.
Regulated cell death joins in atherosclerotic plaque silent progression
Authors: Uyy Elena , Suica Viorel I. , Boteanu Raluca M. , Cerveanu-Hogas Aurel , Ivan Luminita , Hansen Rune , Antohe Felicia 
Published: February 2022

AbstractNon-apoptotic regulated cell death (ferroptosis and necroptosis) leads to the release of damage-associated molecular patterns (DAMPs), which initiate and perpetuate a non-infectious inflammatory response. We hypothesize that DAMPs and non-apoptotic regulated cell death are critical players of atherosclerotic plaque progression with inadequate response to lipid-lowering treatment. We aimed to uncover the silent mechanisms that govern the existing residual risk of cardiovascular-related mortality in experimental atherosclerosis. Proteomic and genomic approaches were applied on the ascending aorta of hyperlipidemic rabbits and controls with and without lipid-lowering treatment. The hyperlipidemic animals, which presented numerous heterogeneous atherosclerotic lesions, exhibited high concentrations of serum lipids and increased lipid peroxidation oxidative stress markers. The analyses revealed the significant upregulation of DAMPs and proteins implicated in ferroptosis and necroptosis by hyperlipidemia. Some of them did not respond to lipid-lowering treatment. Dysregulation of five proteins involved in non-apoptotic regulated cell death proteins (VDAC1, VDAC3, FTL, TF and PCBP1) and nine associated DAMPs (HSP90AA1, HSP90AB1, ANXA1, LGALS3, HSP90B1, S100A11, FN, CALR, H3-3A) was not corrected by the treatment. These proteins could play a key role in the atherosclerotic silent evolution and may possess an unexplored therapeutic potential. Mass spectrometry data are available via ProteomeXchange with identifier PXD026379.
Association between depression and anxiety on symptom and function after surgery for lumbar spinal stenosis
Authors: Held U. , Burgstaller J. M. , Deforth M. , Steurer J. , Pichierri G. , Wertli M. M. 
Published: February 2022

AbstractEvidence on the role of depression and anxiety in patients undergoing surgical treatment for symptomatic degenerative lumbar spinal stenosis (DLSS) is conflicting. We aimed to assess the association between depression and anxiety with symptoms and function in patients undergoing surgery for DLSS. Included were patients with symptomatic DLSS participating in a prospective multicentre cohort study who underwent surgery and completed the 24-month follow-up. We used the hospital anxiety and depression scale (HADS) to assess depression/anxiety. We used mixed-effects models to quantify the impact on the primary outcome change in the spinal stenosis measure (SSM) symptoms/function subscale from baseline to 12- and 24-months. Logistic regression analysis was used to quantify the odds of the SSM to reach a minimal clinically important difference (MCID) at 24 months follow-up. The robustness of the results in the presence of unmeasured confounding was quantified using a benchmarking method based on a multiple linear model. Out of 401 patients 72 (17.95%) were depressed and 80 anxious (19.05%). Depression was associated with more symptoms (β = 0.36, 95% confidence interval (CI) 0.20 to 0.51, p < 0.001) and worse function (β = 0.37, 95% CI 0.24 to 0.50, p < 0.001) at 12- and 24-months. Only the association between baseline depression and SSM symptoms/function was robust at 12 and 24 months. There was no evidence for baseline depression/anxiety decreasing odds for a MCID in SSM symptoms and function over time. In patients undergoing surgery for symptomatic DLSS, preoperative depression but not anxiety was associated with more severe symptoms and disability at 12 and 24 months.
Causes of functional low vision in a Brazilian rehabilitation service
Authors: Ferreira Manuela Molina , Antunes-Foschini Rosalia , Furtado João M. 
Published: February 2022

AbstractThere is limited information on functional low vision (FLV) in Latin America, especially in individuals under 50 years of age. In the present study, we retrospectively evaluated the medical records of 1393 consecutive subjects seen at a Brazilian tertiary rehabilitation service, from February 2009 to June 2016. We collected sociodemographic, clinical data, and information on optical aids and spectacle prescription. Subjects were divided into three age groups: 0 to 14 years old (children), 15 to 49 years old (young adults), and 50 years or older (older adults). The main etiologies leading to FLV in children were cerebral visual impairment (27.9%), ocular toxoplasmosis (8.2%), and retinopathy of prematurity (7.8%). In young adults, retinitis pigmentosa (7.4%) and cone/rod dystrophy (6.5%) were the most frequent, while in older adults, age-related macular degeneration (25.3%) and diabetic retinopathy (18.0%) were the leading causes. Our results indicate that preventable diseases are important causes of FLV in children in the area, and proper prenatal care could reduce their burden. The increasing life expectancy in Latin America and the diabetes epidemic are likely to increase the demand for affordable, people-centered rehabilitation centers, and their integration into health services should be planned accordingly.
Realizing the full reservoir operation potential during the 2020 Yangtze river floods
Authors: Zhang Hairong , Dou Yanhong , Ye Lei , Zhang Chi , Yao Huaming , Bao Zhengfeng , Tang Zhengyang , Wang Yongqiang , Huang Yukai , Zhu Shuang , Xie Mengfei , Wu Jiang , Shi Chao , Ren Yufeng , Zhang Dongjie , Wu Biqiong , Chen Yufan 
Published: February 2022

AbstractFive severe floods occurred in the Yangtze River Basin, China, between July and August 2020, and the Three Gorges Reservoir (TGR) located in the middle Yangtze River experienced the highest inflow since construction. The world’s largest cascade-reservoir group, which counts for 22 cascade reservoirs in the upper Yangtze River, cooperated in real time to control floods. The cooperation prevented evacuation of 600,000 people and extensive inundations of farmlands and aquacultural areas. In addition, no water spillage occurred during the flood control period, resulting in a world-record annual output of the TGR hydropower station. This work describes decision making challenges in the cooperation of super large reservoir groups based on a case-study, controlling the 4th and 5th floods (from Aug-14 to Aug-22), the efforts of technicians, multi-departments, and the state, and reflects on these. To realize the full potential of reservoir operation for the Yangtze River Basin and other basins with large reservoir groups globally, we suggest: (i) improve flood forecast accuracy with a long leading time; (ii) strengthen and further develop ongoing research on reservoir group cooperation; and (iii) improve and implement institutional mechanisms for coordinated operation of large reservoir groups.