bluuebunny/crossref_metadata_2025_split huggingface dataset:
Meningkatkan Kemampuan Siswa Dalam Menjumlahkan Pecahan Campuran Menggunakan Metode Drill Di Kelas V
Authors: Setiawan Muhran 
Published: May 2022

Penelitian tindakan kelas bertujuan secara deskriptif untuk mengetahui apakah ada pengaruh penerapan metode drill terhadap peningkatan kemampuan siswa dalam menjumlahkan dan mengurangkan pecahan campuran pada mata pelajaran matematika kelas V. Subjek yang diberi tindakan adalah siswa kelas V SD Negeri 6 Menteng yang berjumlah 20 orang. Perlakuan tindakan yang diberikan adalah dengan menggunakan metode drill dalam pembelajaran. Harapannya setelah menerapkan metode drill dalam pembelajaran maka nantinya kemampuan siswa dalam menjumlahkan dan mengurangkan pecahan campuran jadi meningkat.
Untuk mengetahui adanya peningkatan kemampuan siswa dalam mengerjakan soal penjumlahan dan pengurangan pecahan maka dilakukan tes uji coba yang dilaksanakan 3 kali kemudian hasilnya dianalisis. Hasil penelitian secara deskriptif menunjukkan bahwa terdapat kecenderungan prestasi belajar matematika siswa kelas V SD Negeri 6 Menteng ada peningkatan, yakni dengan perolehan skor rata � rata yang diperoleh sebelum diadakan tindakan sebesar 61,9 dan setelah diadakan tindakan menjadi 84 dengan demikian berarti ada peningkatan prestasi sebesar 22,1 dengan ketuntasan belajar mencapai 90%.
Meningkatkan Hasil Belajar Matematika Pokok Bahasan Alat Ukur Dalam Tema Kesehatan Melalui Media Konkrit Pada Siswa Kelas III SDN Talian Kereng
Authors: Juriah Agustinah Siti 
Published: May 2022

ABSTRAK
Media pembelajaran sangat berpengaruh terhadap keberhasilan kegiatan belajar mengajar, khususnya terhadap hasil belajar siswa. Untuk itu, penggunaan madia pembelajaran harus lebih mengutamatan keaktivan siswa. Dengan demikian siswa mudah untuk memehami suatu materi pembelajaran.Pelajaran matematika masih dipandang sebagai pelajaran yang sulit, membosankan, dan menakutkan. Untuk itu, media pembelajaran yang dapat dijadikan alternativ oleh guru adalah media konkrit. Dengan media konkrit, siswa dapat dengan mudah memahami isi materi pembelajaran, karena siswa mengetahui secara langsung bentuk nyata dari isi materi yang disampaikan oleh guru.
Tujuan penelitian ini untuk membuktikan adanya peningkatan hasil belajar siswa kelas III SDN Talian Kereng Katingan, setelah menggunakan media konkrit berupa meteran, timbangan, dan jam dinding dalam pelajaran matematika pada pokok bahasan alat ukur.Data awal yang diperoleh dalam penelitian ini menyebutkan bahwa dari 32 siswa hanya 12 anak memperoleh nilai di atas 65 atau dinyatakan tuntas dalam belajar dan 20 anak belum tuntas dalam belajar, karena nilai yang diperoleh masih di bawah 65.Siklus I data yang diperoleh menunjukkan peningkatan hasil belajar siswa. Data yang diperoleh menyebutkan dari 32 siswa, 21 anak dinyatakan tuntas dalam belajar dan 11 anak belum tuntas dalam belajar. Dari hasil tersebut, maka dilanjutkan pada siklus II dan hasilnya menyebutkan peningkatan hasil belajar siswa yang signifikan, yaitu siswa tuntas 100 %.
SPION based nanoformulations: bio-inspired design and functionalization strategies for applications in medicine
Authors: Tietze Rainer , Alexiou Christoph 
Published: May 2022

<img src=” https://s3.amazonaws.com/production.scholastica/article/35650/large/photo-1597423498323-e7e7554b83c6?1652369616”> Biomedical applications in medicine are an area of research studied in great breadth, covering the various potential applications. Mimicking natural processes promises to be a successful strategy for several reasons. For example, physiological structures can generate drug delivery platforms that are better tolerated by the immune system. Furthermore, physiological movement patterns in flagella or contracting motions, which originate from unicellular organisms, are imi-tated. Further examples are stimuli-responsive tissue structures that serve the regeneration of bone material by stimulating pre-osteoblasts to proliferate. The examples of bioinspired SPIONs discussed in this review span the fields of drug delivery, imaging/diagnostics, nano/micromotion, and tissue engineering. All these concepts need to be evaluated regarding their technical feasibility and translatability into the clinic. Therefore, this text concludes with a general consideration for clinically proving such developments.
Pemanfaatan Media Sosial Sebagai Sarana Pembentukan Karakter Kristiani Tingkat Sekolah Menengah Pertama
Authors: Saingo Yakobus Adi 
Published: May 2022

Tujuan penelitian ini adalah untuk mengkaji peran guru Pendidikan Agama Kristen dalam pemanfaatan media sosial sebagai sarana pembentukan karakter kristiani di tingkat Sekolah Menengah Pertama. Dimana pembahasannya juga akan diperdalam dengan mendeskripsikan mengenai bentuk media sosial yang sering digunakan oleh guru Pendidikan Agama Kristen, penggunaan media sosial secara umum oleh guru Pendidikan Agama Kristen, serta dampak penyalahgunaan media sosial oleh peserta didik ketika tidak mendapatkan arahan dan bimbingan yang benar dalam pemanfaatam teknologi digital (khususnya media sosial) oleh guru Pendidikan Agama Kristen. Metode peneltian yang digunakan adalah studi kepustakaan dengan pendekatan deskriptif untuk menggambarkan keadaan umum yang dialami guru Pendidikan Agama Kristen dan peserta didik terkait penggunaan media sosial. Subjek penelitian ini adalah guru Pendidikan Agama Kristen dan peserta didik di lingkungan sekolah. Dalam kajian pustaka ini, peneliti juga memberikan solusi mengenai upaya guru Pendidikan Agama Kristen dalam pemanfaatan media sosial sebagai sarana pembentukan karakter kristiani peserta didik di tingkat Sekolah Menengah Pertama.
¿Es útil la intervención psicológica en las Unidades de Rehabilitación Cardíaca?
Authors: Valls Lázaro Esperanza 
Published: May 2022

Las enfermedades cardiovasculares (ECV) están reduciendo su impacto gracias a la prevención y control de sus factores de riesgo. La rehabilitación cardíaca pretende fomentar una gestión saludable de dichos factores, aunque, en España, la dotación de programas de rehabilitación (PRC) está por debajo de lo recomendado y menor aún el porcentaje que incluye asistencia psicológica. Este estudio examina los resultados obtenidos en factores psicosociales de riesgo cardiaco en 905 pacientes cardíacos adscritos a un PRC multidisciplinar, según la participación o no en un programa psicológico (PP) incluido en el programa general. Resultados: Los participantes del PP (76.6%) mejoraron significativamente en todas las variables evaluadas, con tamaños del efecto moderados, destacando mejorías en depresión, ira, ansiedad y calidad de vida.Los no participantes en PP(23.4%) obtuvieron resultados más limitados y tamaños del efecto menores. Conclusión: Se resalta la contribución beneficiosa de la intervención psicológica en el marco de los PRC por lo que se reclama aumentar la presencia de dichas actuaciones.
Inflammatory Microenvironment Accelerates Bone Marrow Mesenchymal Stem Cell Aging
Authors: Peng Xin , Zhou Xin , Yin Ying , Luo Beibei , Liu Yang , Yang Cheng 
Published: May 2022

MSC senescence is considered a contributing factor in aging-related diseases. We investigated the influence of the inflammatory microenvironment on bone marrow mesenchymal stem cells (BMSCs) under aging conditions and the underlying mechanism to provide new ideas for stem cell therapy for age-related osteoporosis. The BMSCs were cultured until passage 3 (P3) (young group) and passage 10 (P10) (aging group) in vitro. The supernatant was collected as the conditioned medium (CM). The young BMSCs were cultured in the CM of P3 or P10 cells. The effects of CM from different groups on the aging and stemness of the young BMSCs were examined. A Quantibody® mouse inflammation array on serum extracts from young (aged 8 weeks) and old (aged 78 weeks) mice was performed, and differentially expressed factors were screened out. We discovered that the CM from senescent MSCs changed the physiology of young BMSCs. Systemic inflammatory microenvironments changed with age in the mice. In particular, the pro-inflammatory cytokine IL-6 increased, and the anti-inflammatory cytokine IL-10 decreased. The underlying mechanism was investigated by GO and KEGG analyses, and there was a change in the JAK-STAT signaling pathway, which is closely related to IL-6 and IL-10. Collectively, our results demonstrated that the age-related inflammatory microenvironment has a significant effect on the biological functions of BMSCs. Targeted reversal of this inflammatory environment may provide a new strategy for stem cell therapy to treat aging-related skeletal diseases.
New Prognostic Gene Signature and Immune Escape Mechanisms of Bladder Cancer
Authors: Jiang Yi , Zeng Zhenhao , Xiong Situ , Jiang Ming , Huang Gaomin , Zhang Chiyu , Xi Xiaoqing 
Published: May 2022

Background: The immune microenvironment profoundly affects tumor prognosis and therapy. The present study aimed to reveal potential immune escape mechanisms and construct a novel prognostic signature via systematic bioinformatic analysis of the bladder cancer (BLCA) immune microenvironment.Patients and Methods: The transcriptomic data and clinicopathological information for patients with BLCA were obtained from The Cancer Genome Atlas (TCGA). Consensus clustering analysis based on the CIBERSORT and ESTIMATE algorithms was performed with patients with BLCA, which divided them into two clusters. Subsequently, the differentially expressed genes (DEGs) in the two were subjected to univariate Cox and least absolute shrinkage and selection operator (LASSO) regression analyses to identify prognostic genes, which were used to construct a prognostic model. The predictive performance of the model was verified by receiver operating characteristic (ROC) and Kaplan–Meier (K-M) analyses. In addition, we analyzed the differentially altered immune cells, mutation burden, neoantigen load, and subclonal genome fraction between the two clusters to reveal the immune escape mechanism.Results: Based on the ESTIMATE and clustering analyses, patients with BLCA were classified into two heterogeneous clusters: ImmuneScoreH and ImmuneScoreL. Univariate Cox and LASSO regression analyses identified CD96 (HR = 0.83) and IBSP (HR = 1.09), which were used to construct a prognostic gene signature with significant predictive accuracy. Regarding potential immune escape mechanisms, ImmuneScoreH and ImmuneScoreL were characterized by inactivation of innate immune cell chemotaxis. In ImmuneScoreL, a low tumor antigen load might contribute to immune escape. ImmuneScoreH featured high expression of immune checkpoint molecules.Conclusion: CD96 and IBSP were considered prognostic factors for BLCA. Innate immune inactivation and a low tumor antigen load may be associated with immune escape mechanisms in both clusters. Our research complements the exploration of the immune microenvironment in BLCA.
Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide
Authors: Guo Yang , Feng Liang 
Published: May 2022

Tumor markers are one of the important indicators for early cancer diagnosis. As a new analytical method, electrochemical immunosensing analysis has the advantages of high sensitivity, good selectivity, and rapid detection, which is of great significance for the detection of tumor markers. In this work, an AuNP/reduced graphene oxide (AuNP/rGO) composite was synthesized. We used it for electrochemical sensor fabrication with the assistance of the biotin–streptavidin protein (SA) system to further amplify the signal to achieve sensitive detection of carcinoembryonic antigen (CEA). In addition, AuNPs have been incorporated due to their good electrical conductivity and biocompatibility, which can accelerate electron transfer at the electrode interface and improve the loading capacity to capture antibodies. The fabricated AuNPs/SA/rGO has a large working surface area and high material utilization ratio, which improves the catalytic capacity of H2O2 reduction and effectively amplifies the current signal. The linear range of the response current signal of the sensor toward the CEA concentration is 20 fg/ml to 200 ng/ml, and the limit of detection can achieve 6.2 fg/ml. In addition, the fabricated immunosensor has good reproducibility, selectivity, and stability.
QuantiFERON-Cytomegalovirus Assay for Prediction of Cytomegalovirus Viremia in Kidney Transplant Recipients: Study From High Cytomegalovirus Seroprevalence Country
Authors: Pongsakornkullachart Kritsada , Chayakulkeeree Methee , Vongwiwatana Attapong , Kantakamalakul Wannee , Skulratanasak Peenida , Phoompoung Pakpoom 
Published: May 2022

BackgroundEarly studies showed the utility of pretransplant QuantiFERON-Cytomegalovirus (QF-CMV) assays for CMV-disease prediction post kidney transplant (KT). However, recent data are conflicting.MethodsThis prospective cohort study enrolled adult patients undergoing KT between July 2017 and May 2019. Patients with antithymocyte globulin therapy or negative pretransplant CMV IgG were excluded. QF-CMV assays were performed on transplantation day and one month thereafter, and CMV viral loads were obtained 1, 3, and 6 months posttransplantation. The primary outcome was CMV viremia within 6 months. The QF-CMV assay–posttransplant CMV viremia association was analyzed.Results Fifty-five patients were enrolled (male, 58.2%; mean (SD) age, 46.5 (10.2) years). Fifty-two (94.5%) received CMV-seropositive donor kidneys. Over 6 months, 29 patients developed CMV viremia (52.7%), with 14 (25.5%) having significant viremia requiring antiviral therapy. The CMV-viremia incidence of patients with nonreactive and reactive baseline QF-CMV assays did not differ significantly (55.3% and 47.1%; p = 0.573). Among patients with reactive pretransplant QF-CMV assays, there was a trend toward a lower incidence of CMV viremia for those who were persistently reactive at 1 month after KTs, although there was no statistically significant difference (50% vs 83%; p = 0.132).ConclusionsOur study could not support the use of single-timepoint pretransplant or 1-month posttransplant QF-CMV assays as a predictor for posttransplant CMV viremia in CMV seropositive KT recipients. Investigation of the association between dynamic QF-CMV-status changes and CMV-viremia incidence are needed.
Association of QTc Interval and V4-S Wave With Appropriate ICD Therapy in Hypertrophic Cardiomyopathy
Authors: Zhang Nixiao , Cheng Sijing , Niu Hongxia , Gu Min , Peng Hui , Sun Zhijun , Liu Xi , Deng Yu , Chen Xuhua , Hua Wei 
Published: May 2022

BackgroundVentricular arrhythmias in patients with hypertrophic cardiomyopathy (HCM) may lead to sudden cardiac death (SCD). We aimed to investigate the relationship between electrocardiogram (ECG) indicators and the risk of appropriate implantable cardioverter-defibrillator (ICD) therapy in HCM.MethodsThe HCM patients receiving ICD implantation were enrolled consecutively. QT interval correction (QTc) was calculated using Bazett's formula. Long or deep S wave in V4 lead was defined as duration time &gt;50 ms and/or voltage amplitude &gt;0.6 mV. The endpoint in our study was at least one ICD appropriate therapy triggered by ventricular tachyarrhythmia (VT) or ventricular fibrillation (VF), including anti-tachyarrhythmia pacing (ATP) and electrical shock.ResultsA total of 149 patients with HCM (mean age 53 ± 14 years, male 69.8%) were studied. Appropriate ICD therapies occurred in 47 patients (31.5%) during a median follow-up of 2.9 years. Cox regression analysis showed that long or deep S wave in V4 lead [hazard ratio (HR) 1.955, 95% confidence interval (CI) 1.017–3.759, P = 0.045] and QTc interval (HR 1.014, 95% CI 1.008–1.021, P &lt; 0.001) were independent risk factors for appropriate ICD therapy. The ROC showed that the optimal cut-off point value for the QTc interval to predict the appropriate ICD therapy was 464 ms, and the AUC was 0.658 (95% CI 0.544–0.762, P = 0.002). The AUC for S wave anomalies in V4 lead was 0.608 (95% CI 0.511–0.706, P = 0.034). We developed a new model that combined the QTc interval and S wave anomalies in V4 lead based on four patient groups. Patients with QTc ≥464 ms and long or deep V4-S wave had the highest risk of developing appropriate ICD therapy (log-rank P &lt; 0.0001). After adding QTc interval and V4-S wave anomalies into the HCM-risk-SCD model, the prediction effect of the new model was significantly improved, and the NRI was 0.302.ConclusionsIn this HCM cohort, QTc and S wave anomalies in V4 lead were found to be significant and strong predictors of the risk of appropriate ICD therapy. Patients with QTc ≥464 ms and long or deep S wave had the highest risk. After QTc interval and V4-S wave anomalies adding to the HCM-risk-SCD model, the prediction effect is significantly improved.
ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress
Authors: Chi Ani , Yang Bicheng , Cao Xiaohui , Wang Zhenqing , Liu Hanchao , Dai Hao , Deng Chunhua , Zhang Min 
Published: May 2022

Testicular torsion-detorsion is an ischaemia-reperfusion-induced male gonad injury that may lead to male infertility. Oxidative stress plays an important role in the ischaemia-reperfusion injury. Icariside II (ICA II) prevents oxidative stress and has obvious protective effects on spermatogenic function. The present study was aimed to investigate therapeutic potentials of ICA II on testicular torsion. 72 mice were randomly divided into three groups: sham-operated control group (n = 24), testicular ischemia-reperfusion + saline group (n = 24) and testicular ischemia-reperfusion + icariside II treated group (n = 24). Testicular ischemia-reperfusion was induced by the left testis rotated 360 degrees in a clockwise direction for 30 minutes followed by detorsion, the contralateral testis was removed. ICA II in saline (5 mg/kg/day) was administrated by gavage immediately after detorsion. The results demonstrated that ICA II alleviated testicular damage by mitigating spermatogenic cell injury and improving testosterone production in mouse models of testicular torsion. We revealed that ICA II alleviated oxidative stress and apoptosis in the testes, reduced inflammatory infiltration and accelerated angiogenesis. Briefly, ICA II administration ameliorated testicular damage by improving spermatogenic function and testosterone production, which supports its use as a pharmacological treatment of testicular torsion.
Base Editing in Peanut Using CRISPR/nCas9
Authors: Neelakandan Anjanasree K. , Subedi Binita , Traore Sy M. , Binagwa Papias , Wright David A. , He Guohao 
Published: May 2022

Peanut (Arachis hypogaea L.), an allotetraploid legume of the Fabaceae family, is able to thrive in tropical and subtropical regions and is considered as a promising oil seed crop worldwide. Increasing the content of oleic acid has become one of the major goals in peanut breeding because of health benefits such as reduced blood cholesterol level, antioxidant properties and industrial benefits such as longer shelf life. Genomic sequencing of peanut has provided evidence of homeologous AhFAD2A and AhFAD2B genes encoding Fatty Acid Desaturase2 (FAD2), which are responsible for catalyzing the conversion of monounsaturated oleic acid into polyunsaturated linoleic acid. Research studies demonstrate that mutations resulting in a frameshift or stop codon in an FAD2 gene leads to higher oleic acid content in oil. In this study, two expression vectors, pDW3873 and pDW3876, were constructed using Cas9 fused to different deaminases, which were tested as tools to induce point mutations in the promoter and the coding sequences of peanut AhFAD2 genes. Both constructs harbor the single nuclease null variant, nCas9 D10A, to which the PmCDA1 cytosine deaminase was fused to the C-terminal (pDW3873) while rAPOBEC1 deaminase and an uracil glycosylase inhibitor (UGI) were fused to the N-terminal and the C-terminal respectively (pDW3876). Three gRNAs were cloned independently into both constructs and the functionality and efficiency were tested at three target sites in the AhFAD2 genes. Both constructs displayed base editing activity in which cytosine was replaced by thymine or other bases in the targeted editing window. pDW3873 showed higher efficiency compared to pDW3876 suggesting that the former is a better base editor in peanut. This is an important step forward considering introgression of existing mutations into elite varieties can take up to 15 years making this tool a benefit for peanut breeders, farmers, industry and ultimately for consumers.
Whole-Genome/Exome Sequencing Uncovers Mutations and Copy Number Variations in Primary Diffuse Large B-Cell Lymphoma of the Central Nervous System
Authors: Zhu Qiong , Wang Jianchao , Zhang Wenfang , Zhu Weifeng , Wu Zaizeng , Chen Yanping , Chen Musheng , Zheng Limei , Tang Jianqing , Zhang Sheng , Wang Di , Wang Xingfu , Chen Gang 
Published: May 2022

Background/objective: Identification of key genetic alterations is of importance in the targeted therapies of primary central nervous system lymphoma (PCNSL). However, only a small number of studies have been carried out in PCNSL. In this study, we further described the genetic mutations and copy number variations (CNVs) in PCNSL patients using whole-genome/exome sequencing (WGS/WES), as well as revealed their associations with patients’ clinicopathological features and prognosis.Methods: Tumor specimens from 38 patients with primary diffuse large B-cell lymphoma of the central nervous system (CNS DLBCL) were enrolled to WGS (n = 24) or WES (n = 14). The CNVs and mutations of 24 samples (WGS) and 38 samples (WGS/WES) were characterized, respectively. The associations between CNVs and mutations with the overall survival rates of PCNSL patients were also evaluated.Results: The most common mutations were identified in IGLL5 (68%), PIM1 (63%), MYD88 (55%), CD79B (42%), BTG2 (39%), PCLO (39%), KMT2D (34%), and BTG1 (29%) genes. Among the mutated genes, EP300, ETV6, and HIST1H1E mutations were exclusively detected in the elderly, while DUSP2 mutations were associated with the immune microenvironment indicators. In addition, KMT2D mutation was associated with a poor prognosis. In addition, 488 CNVs including 91 gains and 397 deletions were observed across 24 samples from WGS results. Notably, 1q31.3 amplification was closely associated with the poor prognosis of PCNSL patients.Conclusion: This study further characterizes the genomic landscape of primary CNS DLBCL using WGS/WES, which provides insight into understanding the pathogenesis of PCNSL and fosters new ideas for the targeted treatment of PCNSL.