bluuebunny/crossref_metadata_2025_split huggingface dataset:
Energy status-promoted growth and development of Arabidopsis require copper deficiency response transcriptional regulator SPL7
Authors: Schulten Anna , Pietzenuk Björn , Quintana Julia , Scholle Marleen , Feil Regina , Krause Marcus , Romera-Branchat Maida , Wahl Vanessa , Severing Edouard , Coupland George , Krämer Ute 
Published: July 2022

AbstractCopper (Cu) is a cofactor of around 300 Arabidopsis proteins, including photosynthetic and mitochondrial electron transfer chain enzymes critical for adenosine triphosphate (ATP) production and carbon fixation. Plant acclimation to Cu deficiency requires the transcription factor SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7). We report that in the wild type (WT) and in the spl7-1 mutant, respiratory electron flux via Cu-dependent cytochrome c oxidase is unaffected under both normal and low-Cu cultivation conditions. Supplementing Cu-deficient medium with exogenous sugar stimulated growth of the WT, but not of spl7 mutants. Instead, these mutants accumulated carbohydrates, including the signaling sugar trehalose 6-phosphate, as well as ATP and NADH, even under normal Cu supply and without sugar supplementation. Delayed spl7-1 development was in agreement with its attenuated sugar responsiveness. Functional TARGET OF RAPAMYCIN and SNF1-RELATED KINASE1 signaling in spl7-1 argued against fundamental defects in these energy-signaling hubs. Sequencing of chromatin immunoprecipitates combined with transcriptome profiling identified direct targets of SPL7-mediated positive regulation, including Fe SUPEROXIDE DISMUTASE1 (FSD1), COPPER-DEFICIENCY-INDUCED TRANSCRIPTION FACTOR1 (CITF1), and the uncharacterized bHLH23 (CITF2), as well as an enriched upstream GTACTRC motif. In summary, transducing energy availability into growth and reproductive development requires the function of SPL7. Our results could help increase crop yields, especially on Cu-deficient soils.
Radiofrequency Ablation of the Infrapatellar Branch of the Saphenous Nerve for the Treatment of Chronic Anterior Inferomedial Knee Pain
Authors: Beckwith Margaret , Cushman Daniel , Clark Tyler , Park Peter K , Burnham Taylor , Burnham Robert , McCormick Zachary L , Conger Aaron 
Published: July 2022

Abstract
Intro. Genicular nerve radiofrequency ablation (GNRFA) is an effective treatment for chronic knee pain related to osteoarthritis. It is often utilized when conservative management has failed and patients wish to avoid arthroplasty, are poor surgical candidates due to comorbid medical conditions, or in those suffering from persistent pain after arthroplasty. The classic targets for GNRFA include the superior lateral genicular nerve, superior medial genicular nerve, and inferior medial genicular nerve but multiple anatomic studies have demonstrated additional sensory innervation to the knee. Objective. In this research article, we propose an image-guided technique that can safely target the infrapatellar branch of the saphenous nerve which also provides sensory innervation to the anterior capsule. Proposal. The proposed technique includes variations for conventional bipolar radiofrequency ablation, cooled radiofrequency ablation, dual-tined bipolar radiofrequency ablation, and monopolar radiofrequency ablation using a long axis approach. The described technique is based on updated anatomic studies and takes into account safety concerns such as thermal risk to the skin and/or pes anserine tendons and breaching of the synovial cavity. Conclusion. Future clinical research should be performed to confirm the safety and effectiveness of this specific approach.
The Pain Clinic for Older People
Authors: Chen Nancy , Farrell Michael , Kendall Sarah , Levy Leah , Mehan Robert , Katz Benny 
Published: July 2022

Abstract

Objective
Multidisciplinary pain clinics have an established role in the management of persistent pain, but there is little evidence to support this approach in an older population. This study describes the characteristics and pain outcomes of patients attending a pain clinic designed exclusively for older people.

Methods
A retrospective audit was performed of outcomes of the Pain Clinic for Older People (PCOP) in 2015–2019. Response to treatment was determined by change in Brief Pain Inventory (BPI) scores at initial attendance and after a treatment program. Clinically meaningful improvement was defined by the Initiative on Methods, Measurement, and Pain Assessment in Clinical Trials (IMMPACT) consensus criteria of ≥30% improvement in average pain and one-point improvement in pain interference. Results were compared with the national benchmark collated by the electronic Persistent Pain Outcomes Collaboration (ePPOC), which reports the combined results from 67 participating Australian and New Zealand pain services.

Results
Patients attending the PCOP had a mean age of 80.5 years and had high rates of frailty (84%), cognitive impairment (30%), and multimorbidity. Significant reductions in BPI average pain and BPI pain interference scores were achieved. Clinically meaningful improvement in BPI average pain was achieved in 63% of patients attending the PCOP who were 65–74 years of age and in 46% of patients who were ≥75 years of age, which met the national benchmark set by ePPOC of 40% for both age groups. Clinically meaningful improvement in BPI pain interference was achieved in 69% of those attending the PCOP who were 65–74 years of age and in 66% of those who were ≥75 years of age, comparable to the ePPOC benchmark of 71% and 65% for the respective age groups.

Conclusion
PCOP clients achieved significant and meaningful improvements in their pain outcomes that satisfied the national benchmark. Advanced age, cognitive impairment, frailty and multimorbidity should not be regarded as barriers to benefit from a pain clinic specifically designed for older people.
Quantifying navigation complexity in transportation networks
Authors: Jiang Zhuojun , Dong Lei , Wu Lun , Liu Yu 
Published: July 2022

Abstract
The complexity of navigation in cities has increased with the expansion of urban areas, creating challenging transportation problems that drive many studies on the navigability of networks. However, due to the lack of individual mobility data, large-scale empirical analysis of the wayfinder’s real-world navigation is rare. Here, using 225 million subway trips from three major cities in China, we quantify navigation difficulty from an information perspective. Our results reveal that (1) people conserve a small number of repeatedly used routes and (2) the navigation information in the subnetworks formed by those routes is much smaller than the theoretical value in the global network, suggesting that the decision cost for actual trips is significantly smaller than the theoretical upper limit found in previous studies. By modeling routing behaviors in growing networks, we show that while the global network becomes difficult to navigate, navigability can be improved in subnetworks. We further present a universal linear relationship between the empirical and theoretical search information, which allows the two metrics to predict each other. Our findings demonstrate how large-scale observations can quantify real-world navigation behaviors and aid in evaluating transportation planning.
A flux-based machine learning model to simulate the impact of pathogen metabolic heterogeneity on drug interactions
Authors: Chung Carolina H , Chandrasekaran Sriram 
Published: July 2022

AbstractDrug combinations are a promising strategy to counter antibiotic resistance. However, current experimental and computational approaches do not account for the entire complexity involved in combination therapy design, such as the effect of pathogen metabolic heterogeneity, changes in the growth environment, drug treatment order, and time interval. To address these limitations, we present a comprehensive approach that uses genome-scale metabolic modeling and machine learning to guide combination therapy design. Our mechanistic approach (a) accommodates diverse data types, (b) accounts for time- and order-specific interactions, and (c) accurately predicts drug interactions in various growth conditions and their robustness to pathogen metabolic heterogeneity. Our approach achieved high accuracy (area under the receiver operating curve (AUROC) = 0.83 for synergy, AUROC = 0.98 for antagonism) in predicting drug interactions for Escherichia coli cultured in 57 metabolic conditions based on experimental validation. The entropy in bacterial metabolic response was predictive of combination therapy outcomes across time scales and growth conditions. Simulation of metabolic heterogeneity using population FBA identified two subpopulations of E. coli cells defined by the levels of three proteins (eno, fadB, and fabD) in glycolysis and lipid metabolism that influence cell tolerance to a broad range of antibiotic combinations. Analysis of the vast landscape of condition-specific drug interactions revealed a set of 24 robustly synergistic drug combinations with potential for clinical use.
Disparity in female and Asian representation amongst cardiology journal editorial boards members: a call for empowerment
Authors: Lim O Z H , Chen Y , Dimmeler S , Yong A W J , Singh H , Sim M L E , Kong G , Lim W H , Low T T , Park D -W , Chew N W S , Foo R 
Published: July 2022

Summary

Background
While progress is evident in gender and ethnic representation in the workplace, this disparity remains prevalent in academic positions.

Objectives
We examined gender and Asian ethnic representation in editorial boards of cardiology journals.

Methods
A cross-sectional analysis was conducted using publicly available data on Cardiology and Cardiovascular medicine journals in the first quartile of the 2020 Scimago Journal & Country Rank indicator. The proportions of female and Asian editorial board members, associate editors and editors-in-chief were assessed. Subgroup analyses were conducted based on the journal’s geographical origin, subspecialty and demographic of the editor-in-chief.

Results
Seventy-six cardiology journals, involving 8915 editorial board members, were included. Only 19.6% of editorial board members were female, 20.8% Asians and 4.0% Asian females. There were less female representation amongst editors-in-chief (9.9%) compared to associate editors (22.3%). European (18.1%) and North American-based journals (21.1%) had higher female representation compared to Asian-based journals (8.7%). There was lower Asian representation in European (18.1%) and North American-based journals (19.9%) compared to Asian-based journals (72.3%). Females were underrepresented in interventional (14.5%) journals, while Asians were underrepresented in general cardiology (18.3%) and heart failure (18.3%) journals. Journals led by female editors-in-chief had significantly higher female representation compared to male-led ones, while journals with Asian editors-in-chief had greater Asian representation compared to non-Asian led ones.

Conclusion
This study highlights the female and Asian ethnic underrepresentation in academic roles in cardiology journal editorial boards. Further analysis is needed for other ethnicities, while the community pushes towards gender-balanced and ethnic diversity across editorial boards.
Mind your step: social cerebellum in interactive navigation
Authors: Li Meijia , Pu Min , Baetens Kris , Baeken Chris , Deroost Natacha , Heleven Elien , Van Overwalle Frank 
Published: July 2022

AbstractThe posterior cerebellum contributes to dynamic social cognition by building representations and predictions about sequences in which social interactions typically take place. However, the extent to which violations of prior social expectations during human interaction activate the cerebellum remains largely unknown. The present study examined inconsistent actions, which violate the expectations of desired goal outcomes, by using a social navigation paradigm in which a protagonist presented a gift to another agent that was liked or not. As an analogous non-social control condition, a pen was transported via an assembly line and filled with ink that matched the pen’s cap or not. Participants (n = 25) were required to memorize and subsequently reproduce the sequence of the protagonist’s or pen’s trajectory. As hypothesized, expectation violations in social (vs non-social) sequencing were associated with activation in the posterior cerebellum (Crus 1/2) and other cortical mentalizing regions. In contrast, non-social (vs social) sequencing recruited cerebellar lobules IV–V, the action observation network and the navigation-related parahippocampal gyrus. There was little effect in comparison with a social non-sequencing control condition, where participants only had to observe the trajectory. The findings provide further evidence of cerebellar involvement in signaling inconsistencies in social outcomes of goal-directed navigation.
Energy restriction and housing of pregnant beef heifers in mud decreases body weight and conceptus free live weight
Authors: Nickles Kirsten R , Garcia-Guerra Alvaro , Fluharty Francis L , Kieffer Justin D , Relling Alejandro E , Parker Anthony J 
Published: July 2022

AbstractAverage temperatures in the Midwest, USA are predicted to increase 2–9°C by the end of the century; resulting in muddy pastures for spring calving beef heifers as they enter late gestation. The objective of this study was to evaluate the effects of muddy conditions on heifer body weight (BW), body condition score (BCS), conceptus free live weight (CFLW), and fetal growth when heifers were energy restricted during late gestation. Eighteen Angus heifers (n = 9/treatment) were paired based on initial BW. One heifer from each BW pair was randomly allocated to either the mud (MUD) or control (CON) treatment on day 196 of gestation. Pens in the CON treatment were bedded with wood chips, while pens in the MUD treatment were filled with mud (average depth of 19.5 ± 7.9 cm). Heifers were housed individually and fed the same diet that consisted of a limit-fed total mixed ration from day 196 to 266 of gestation that was formulated to meet 66% of the net energy for maintenance, growth, and gestation requirements. Requirements and the amount of the diet offered were adjusted weekly, and heifers were weighed and sampled for blood metabolites weekly. Data were analyzed as a randomized complete block design with repeated measurements. There was a treatment × day of gestation interaction, such that heifers had similar BW, BCS, and CFLW on day 196 of gestation. By day 266 of gestation; however, heifers in the MUD treatment weighed 43.5 kg less (P &lt; 0.01) and were 1.8 BCS units less (P &lt; 0.01) than heifers in the CON treatment. This is further supported by the treatment × day effects we observed for back fat (BF) and rump fat (RF) thickness, such that the MUD heifers had less BF (P = 0.02) and RF (P &lt; 0.01) by day 266 of gestation. There was a marginally significant difference for gestation length (P = 0.06), such that heifers in the MUD treatment calved approximately 3.1 days before the heifers in the CON treatment. Though heifers in the MUD treatment decreased their BW and CFLW during the treatment period, we did not observe a difference in calf birth weight (P = 0.34), calf plasma IgG concentration (P = 0.37), or calf weaning weight (P = 0.63). Despite heifers in the MUD treatment having greater BW, CFLW, and BCS losses compared with the heifers in the CON treatment, the heifers in the MUD treatment seemed to prioritize fetal growth, as they mobilized their body tissues to meet the energetic demands of pregnancy.
Amygdala subnuclei volumes, functional connectivity, and social–emotional outcomes in children born very preterm
Authors: Mueller Megan , Thompson Benjamin , Poppe Tanya , Alsweiler Jane , Gamble Greg , Jiang Yannan , Leung Myra , Tottman Anna C , Wouldes Trecia , Harding Jane E , Duerden Emma G ,   PIANO Study Group
Published: July 2022

AbstractChildren born very preterm can demonstrate social-cognitive impairments, which may result from limbic system dysfunction. Altered development of the subnuclei of the amygdala, stress-sensitive regions involved in emotional processing, may be key predictors of social-skill development. In a prospective cohort study, 7-year-old children born very preterm underwent neurodevelopmental testing and brain MRI. The Child Behavioral Checklist was used to assess social–emotional outcomes. Subnuclei volumes were extracted automatically from structural scans (n = 69) and functional connectivity (n = 66) was examined. General Linear Models were employed to examine the relationships between amygdala subnuclei volumes and functional connectivity values and social–emotional outcomes. Sex was a significant predictor of all social–emotional outcomes (P &lt; 0.05), with boys having poorer social–emotional outcomes. Smaller right basal nuclei volumes (B = -0.043, P = 0.014), smaller right cortical volumes (B = -0.242, P = 0.02) and larger right central nuclei volumes (B = 0.85, P = 0.049) were associated with increased social problems. Decreased connectivity strength between thalamic and amygdala networks and smaller right basal volumes were significant predictors of greater social problems (both, P &lt; 0.05), effects which were stronger in girls (P = 0.025). Dysregulated maturation of the amygdala subnuclei, along with altered connectivity strength in stress-sensitive regions, may reflect stress-induced dysfunction and can be predictive of social–emotional outcomes.
Microtemporal Relationships in the Fear Avoidance Model
Authors: Kichline Tiffany , Cushing Christopher C. , Connelly Mark , Black William R. , Simons Laura E. , Friesen Craig , Schurman Jennifer V. 
Published: July 2022

Objectives:
Current literature in pediatric pain evaluates the Fear Avoidance model (FAM) pathways at the trait (or macrotemporal) level, but it is unknown if these pathways also occur at the state (or microtemporal) level. Identifying microtemporal processes can improve our understanding of how the relationships within the Fear Avoidance constructs vary when specific Fear Avoidance variables wax and wane. We hypothesized that changes in FAM constructs would be associated with changes in the next variable in the sequence on a microtemporal level, including: (1) higher pain when there is more pain-related fear, (2) higher pain-related fear when there is more avoidance, and (3) higher avoidance when there is more reported disability.

Methods:
71 pediatric patients with chronic abdominal pain (M=13.34 y, SD=2.67 y) reported pain severity, pain-related fear, and avoidance via ecological momentary assessments over 14 days.

Results:
Our results indicated significant microtemporal relationships between Fear Avoidance constructs for pain predicting pain-related fear, pain-related fear predicting avoidance, and avoidance predicting disability.

Discussion:
The current study suggests that the ways in which the FAM is related to various aspects of pain functioning differs on a state-level, which adds new clinical and research opportunities.
No Benefits of Adding Dexmedetomidine, Ketamine, Dexamethasone, and Nerve Blocks to an Established Multimodal Analgesic Regimen after Total Knee Arthroplasty
Authors: Muñoz-Leyva Felipe , Jack James M. , Bhatia Anuj , Chin Ki Jinn , Gandhi Rajiv , Perlas Anahi , Jin Rongyu , Chan Vincent 
Published: July 2022

Background
An optimal opioid-sparing multimodal analgesic regimen to treat severe pain can enhance recovery after total knee arthroplasty. The hypothesis was that adding five recently described intravenous and regional interventions to multimodal analgesic regimen can further reduce opioid consumption.

Methods
In a double-blinded fashion, 78 patients undergoing elective total knee arthroplasty were randomized to either (1) a control group (n = 39) that received spinal anesthesia with intrathecal morphine, periarticular local anesthesia infiltration, intravenous dexamethasone, and a single injection adductor canal block or (2) a study group (n = 39) that received the same set of analgesic treatments plus five additional interventions: local anesthetic infiltration between the popliteal artery and capsule of the posterior knee, intraoperative intravenous dexmedetomidine and ketamine, and postoperatively, one additional intravenous dexamethasone bolus and two additional adductor canal block injections. The primary outcome measure was 24-h cumulative opioid consumption after surgery and secondary outcomes were other analgesics, patient recovery, functional outcomes, and adverse events.

Results
Opioid consumption was not different between groups at 24 h (oral morphine equivalents, mean ± SD; study: 23.7 ± 18.0 mg vs. control: 29.3 ± 18.7 mg; mean difference [95% CI], –5.6 mg [–2.7 to 13.9]; P = 0.189) and all other time points after surgery. There were no major differences in pain scores, quality of recovery, or time to reach rehabilitation milestones. Hypotensive episodes occurred more frequently in the study group (25 of 39 [64.1%] vs. 13 of 39 [33.3%]; P = 0.010).

Conclusions
In the presence of periarticular local anesthesia infiltration, intrathecal morphine, single-shot adductor canal block and dexamethasone, the addition of five analgesic interventions—local anesthetic infiltration between the popliteal artery and capsule of the posterior knee, intravenous dexmedetomidine, intravenous ketamine, an additional intravenous dexamethasone dose, and repeated adductor canal block injections—failed to further reduce opioid consumption or pain scores or to improve functional outcomes after total knee arthroplasty.

Editor’s Perspective

What We Already Know about This Topic

What This Article Tells Us That Is New
A Cadaver Feasibility Study of Extradural Contralateral C7 Ventral Root Transfer Technique for Treating Upper Extremity Paralysis
Authors: Chang Jie , Wang Binyu , Yu Lipeng , Wang Tong , Sui Tao , Zhu Yi , Cao Xiaojian , Yang Kaixiang 
Published: July 2022

Study Design.
A total of 6 formalin-fixed cadavers were included in the cadaver feasibility study.

Objective.
The aim was to ascertain the anatomical feasibility of extradural contralateral C7 ventral root transfer technique by cervical posterior.

Summary of Background Data.
Upper limb spastic hemiplegia is a common sequela after stroke. In our previous study, the authors established a method by transferring contralateral C7 dorsal and ventral roots to the corresponding C7 dorsal and ventral roots on the affected side in the cervical posterior.

Methods.
In the present study, six formalin-fixed cadavers were dissected to confirm the anatomical feasibility. Experimental anastomosis in cadavers was conducted. The pertinent lengths of the extradural nerve roots were measured. The tissue structures surrounding regions between the extradural CC7 nerve roots and the vertebral artery were observed. The cervical magnetic resonance imaging scans of 60 adults were used to measure the distance between the donor and recipient nerves.

Results.
Experimental anastomosis showed that the distance between the donor and recipient nerves was approximately 1 cm; the short segment of the sural nerve needed bridging. The distance between both exit sites of the exit of the extradural dura mater was 33.57±1.55 mm. The length of the extradural CC7 ventral root was 22.00±0.98 mm. The ventral distance (vd) and the dorsal distance (dd) in males were 23.98±1.72 mm and 30.85±2.22 mm (P<0.05), while those in females were 23.28±1.51 mm and 30.03±2.16 mm, respectively. C7 vertebral transverse process, ligaments, and other soft tissues were observed between the vertebral artery and the extradural C7 nerve root.

Conclusion.
Under the premise of less trauma, our study shows that the extradural contralateral C7 ventral root transfer technique, in theory, yields better surgical results, including better recovery of motor function and complete preservation of sensory function.

Level of Evidence.
5