bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Novel Hub Genes in Atherosclerosis Induced by Abnormal Endothelial Shear Stress via Bioinformatics Analysis
Authors: Zhu Guoqi , Lai Yan , Chen Fei , Qian Jun , lin Hao , Yuan Deqiang , Yao Tongqing , Liu XueBo 
Published: January 2022

Abstract
Background: Abnormal endothelial shear stress (ESS) is a significant risk factor for atherosclerosis (AS); however, the genes and pathways between ESS and AS are poorly understood. Here, we screened hub genes and potential regulatory targets linked to the progression of AS induced by abnormal ESS. Methods: GSE45225, GSE23289 and GSE43292 were downloaded from the Gene Expression Omnibus (GEO) database. The limma package in R was used to identify differentially expressed genes (DEGs). The common DEGs of GSE45225 and GSE43292 were regarded as low-ESS-AS related genes. Co-DEGs obtained from GSE23289 and GSE43292 were considered as high-ESS-AS related genes. Next, Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were conducted. Molecular interaction networks were assembled and their crucial genes were identified using Cytoscape. Our findings were also verified in GSE28829, GSE100927. Results: A total of 85 low-ESS-AS related genes and 118 high-ESS-AS related genes were identified. FBXO32, ICAM1 and TNFRSF1B were identified as key hub genes and validated in the GSE28829, GSE100927. ROC analysis indicates that FBXO32 (AUC=0.782), ICAM1 (AUC=0.822) and TNFRSF1B (AUC=0.801) could effectively distinguish the atherosclerotic plaque and normal arterial. Conclusion: We identified ICAM1, TNFRSF1B and FBXO32 as key genes associated with abnormal ESS and AS and may provide potential prevention and treatment target of AS induced by abnormal ESS.
Long-Term Straw Return Increases Biological Nitrogen Fixation by Increasing Soil Organic Carbon and Decreasing Available Nitrogen in Rice–Rapeseed Rotation
Authors: Fan Huishan , Jia Shengqiang , Yu Man , Chen Xijing , Shen Alin , Su Yao 
Published: January 2022

Abstract
Background

Straw return can affect the soil nitrogen (N) balance by changing soil carbon (C) and N components and contents. To understand how straw return affects soil N fixation capacity, relations between biological N fixation and changes in soil C and N contents at different depths need to be examined.
Method

Soil samples were collected at 20-cm intervals from 0 to 100 cm after 5 years of a rice–rapeseed rotation system with straw return (ST) and without (CK). Organic C and inorganic N contents, potential N fixation rate, and abundance and population composition of N-fixing bacteria were analyzed at different soil depths.
Results

Compared with CK, ST increased soil organic C (SOC), particulate organic C (POC), mineral-associated organic C (MOC), and total N (TN) but decreased nitrate-N (NO3−-N). Straw return also increased abundance of N-fixing bacteria 2.9 to 18.2-fold at depths from 0 to 40 cm and potential N fixation rate at 0–20 cm by 24.78%, which was equivalent to an annual increase in N fixation of approximately 0.01 to 0.54 kg·ha−1. Long-term ST significantly affected the composition of N-fixing bacteria at depths from 0 to 40 cm.
Conclusions

Long-term straw return can effectively increase potential N fixation rate in soil and change the structure of the N-fixing bacterial community by increasing SOC and decreasing N availability. The experiment showed that SOC, POC, TN, and NO3−-N were the main environmental factors driving changes in abundance, activity, and population composition of soil N-fixing bacteria under ST.
Managing Metabolic Risk Factors of Non-Communicable Diseases: A Field Trial
Authors: Asadi-Aliabadi Mehran , Karimi Seyed M , Mirbaha-Hashemi Fariba , Tehrani-Banihashemi Arash , Janani Leila , Babaee Ebrahim , Nojomi Marzieh , Moradi-Lakeh Maziar 
Published: January 2022

Abstract
Background: Non-communicable diseases can be controlled and managed by reducing their associated metabolic risk factors. In this study, a set of intervention packages were designed to reduce the prevalence of metabolic risk factors in the community by motivating non-physician health workers. Methods: A field trial study was conducted in 4 districts of Iran. Thirty-two community health centers were randomly selected in the districts. A survey of 30 to 70-year-old participants in each community health center was conducted to measure baseline metabolic risk factors. The intervention packages focused on improving hypertension, hyperlipidemia, obesity and overweight. The interventions included goal-setting, evidence-based education, operational planning, and incentive payments for non-physician health workers. A second survey to measure the final metabolic risk factors was performed after one year. The difference-in-difference method was used to evaluate the effectiveness of the intervention packages.Results: The average age of participants in both surveys was 49 years, and the sex ratio did not change. In percentage of surveyed individuals with a college education was greater in non-intervention centers. The interventions had statistically significant effects only on decreasing the prevalence of overweight and obesity. The package with all the interventions except pay-for-performance decreased the odds of overweight and obesity to 0.57 (95% CI: 0.34, 0.95). Conclusion: Involving non-physician health workers and having action plans based on the health needs of the covered population, as well as holding training courses, and introducing new prevention and control strategies, can decrease obesity and overweight in the community. However, longer trials are needed to observe the effects on hypertension and hyperlipidemia.Trial Registration: This trial was registered in the Iranian Registry of Clinical Trials (IRCT20081205001488N2) on 3 June 2018 (https://en.irct.ir/trial/774).
Health resource utilization and cost before versus after initiation of second-generation long-acting injectable antipsychotics among adults with schizophrenia in Alberta, Canada: a retrospective, observational single-arm study
Authors: Wong Kai On , Klarenbach Scott W , Martins Karen JB , Chue Pierre , Dursun Serdar M , Snaterse Mark , Guigue Alexis , So Helen , Luu Huong , Richer Lawrence 
Published: January 2022

Abstract
Background

Long-acting injectable (LAI) antipsychotics, along with community treatment orders (CTOs), are used to improve treatment effectiveness through adherence among individuals with schizophrenia. Understanding real-world medication adherence, and healthcare resource utilization (HRU) and costs in individuals with schizophrenia overall and by CTO status before and after second generation antipsychotic (SGA)-LAI initiation may guide strategies to optimize treatment among those with schizophrenia.
Methods

This retrospective observational single-arm study utilized administrative health data from Alberta, Canada. Adults (≥18 years) with schizophrenia who initiated a SGA-LAI (no use in the previous 2-years) between April 1, 2014 and March 31, 2016, and had ≥1 additional dispensation of a SGA-LAI were included; index date was the date of SGA-LAI initiation. Medication possession ratio (MPR) was determined, and paired t-tests were used to examine mean differences in all-cause and mental health-related HRU and costs (Canadian dollars), comprised of hospitalizations, physician visits, emergency department visits, and total visits, over the 2-year post-index and 2-year pre-index periods. Analyses were stratified by presence or absence of an active CTO during the pre-index and/or post-index periods.
Results

Among 1,211 adults with schizophrenia who initiated SGA-LAIs, 64% were males with a mean age of 38 (standard deviation [SD] 14) years. The mean overall antipsychotic MPR was 0.39 (95% confidence interval [CI] 0.36, 0.41) greater during the 2-year post-index period (0.84 [SD 0.26]) compared with the 2-year pre-index period (0.45 [SD 0.40]). All-cause and mental health-related HRU and costs were lower post-index versus pre-index (p<0.001) for hospitalizations, physician visits, emergency department visits, and total visits; mean total all-cause HRU costs were $33,788 (95% CI -$38,993, -$28,583) lower post- versus pre-index ($40,343 [SD $68,887] versus $74,131 [SD $75,941]), and total mental health-related HRU costs were $34,198 (95%CI -$39,098, -$29,297) lower post- versus pre-index ($34,205 [SD $63,428] versus $68,403 [SD $72,088]) per-patient. Forty-three percent had ≥1 active CTO during the study period; HRU and costs varied according to CTO status.
Conclusions

SGA-LAIs are associated with greater medication adherence, and lower HRU and costs however the latter vary according to CTO status.
Clinical Presentation and Surgical Management of Peptic Ulcer Perforation in a Tertiary Hospital in Mogadishu- Somalia: A 5 Years Retrospective Study
Authors: Ali Abdihamid Mohamed , Mohamed Abdulkadir Nor , Mohamed Yahye Garad , Keleşoğlu Salim İdris 
Published: January 2022

Abstract
Background: Peptic ulcer perforation is a common surgical emergency condition worldwide, which is associated with significant morbidity and mortality if early diagnosis and immediate surgical management was not carried out. Perforation occurs in roughly 5% of PUD patients during their lifetime.Methods: A 5 years Retrospective observational study on the clinical Presentation and Surgical Management of Peptic Ulcer Perforation in a Tertiary Hospital in Mogadishu- Somalia, department of General Surgery from January 2017 to December 2021. We included all patients undergoing operations with an intra-operative confirmed diagnosis of peptic ulcer perforation at the general surgery department. For operated patients, follow-up evaluation was performed in the outpatient department.Results: Fifty-one patients underwent an emergency operation for peptic ulcer perforation during the study period. The socio-demographic distribution of patients was 45(88.2%) males and 6 (11.8%) females, giving a male to female ratio of 7.5:1.The mean age of patients was 35.5±16.8 years, and the peak frequency was in the third decade. The commonest presenting symptoms were sudden onset of severe epigastric pain in 42 (82.4%) patients. Patients who presented perforated peptic ulcer within 24 hours of initiation of symptoms were free from complications.Age group and delayed presentation > 48 hours after onset of symptoms were linked to post-operative complications and were statistically significant (P 0.032), (P0.005) respectively. Four patients died (mortality rate of 7.8%). Two patient was reoperated because of the failed primary repair and 4 patients had >5cm intra-abdominal abscess image-guided percutaneous drainage was performed and the rest antibiotic therapy according to peritoneal fluid culture and sensitivity results.The most common microorganism isolated was E. coli 22% and Klebsiella 11%. Other rare microorganisms (pseudomonas, Staphylococcus aureus, and Candida spp) were identified. Half 51% of Peritoneal fluid culture no micro-organism growth was seen.Conclusion: The distribution of peptic ulcer perforation is common in the young age group in the third decades of life. Delayed presentation of the disease is linked because most patients arrived from remote areas where proper facilities of health care and health education are not available and the patient might come to the hospital in an advanced stage of the disease. We suggest conducting further researches, health awareness related to complications over-the-counter drugs self-medication, and bad habit including smoking, and to improve health-seeking behaviors of society.
MED15-TFE3 Renal Cell Carcinoma: a Xp11 Translocation Renal Cell Carcinoma Subtype With Distinct Clinicopathological Features and Superior Prognosis
Authors: Gan Weidong , Dong Xiang , Guo Wei , Zhou Peng , Pan Jun , Guo Hongqian 
Published: January 2022

Abstract
Background: MED15-TFE3 renal cell carcinoma is a rare subtype of the Xp11 translocation renal cell carcinoma. To data, only 14 cases of MED15-TFE3 renal cell carcinoma have been reported worldwide.Methods: MED15-TFE3 gene fusion was identified in two cases of Xp11 translocation renal cell carcinoma by using RNA-sequencing. We reviewed and summarized the clinicopathological characteristics, imaging features and prognosis of the MED15-TFE3 renal cell carcinoma. Results: Morphologically, MED15-TFE3 RCC have the typical cystic-solid (mostly cystic) structure. The tumor cells had round nuclei with inconspicuous nucleoli and abundant clear or eosinophilic cytoplasm. Psammoma bodies were randomly distributed within the stroma. Immunohistochemically, the typical TFE3 positive staining could be observed in both cases. The split red-green signal also revealed the positive results in fluorescence in situ hybridization (FISH) assay. Finally, RNA sequencing detected the existence of MED15-TFE3 gene fusion. One pediatric patient developed renal hilar lymph node metastasis before surgery. However, during the follow-up period, no disease recurrence and metastasis happened to both patients. Conclusions: Different from the traditional Xp11 translocation RCC, MED15-TFE3 RCC may have the distinctive cystic-solid structure and superior prognosis. These findings enhanced our understanding of the heterogeneity in Xp11 translocation RCC.
Identification of Hub Genes and Molecular Mechanism Associated With the Progression of Pediatric Low Grade Gliomas
Authors: Tao Hualin , Zeng Chuyi , Zuo Zhihua 
Published: January 2022

Abstract
Background: To explore hub genes and underlying molecular mechanism about the malignant progression of pediatric low grade gliomas (PLGG). Methods: The microarray were obtained from Gene Expression Omnibus database and hub genes were identified by bioinformatics analysis. Results: Ten hub genes were confirmed to increase with the development of PLGG, and their high expression was correlated with poor prognosis. Additionally, the encoding protein expression level of CDK1, KIF11, TRIP13, MAD2L1, PBK were significantly higher in high grade glioma tissues than that in PLGG. Finally, functional enrichment analysis demonstrated these genes were mainly enriched in cell cycle activities. Conclusion: Our results offer novel insights of the molecular mechanisms and identify potential biomarkers or therapeutic targets for PLGG progression.
The potential effect of blood glucose on the obesity paradox in patients with severe stroke: a retrospective observational study
Authors: Ma Zisheng , Li Shunxian , Lin Xinjiang 
Published: January 2022

Abstract
Background: On the one hand, according to the obesity paradox, patients with excessive BMI will have lower mortality after onset. On the other hand, due to higher levels of glucose induced by excessive BMI, they will suffer a worse prognosis. This is contradictory. In the present study, we aim to prove the obesity paradox in critically ill stroke patients and find out the potential role of increased glucose.Methods: This was a retrospective observational study about patients with acute stroke in the intensive care unit (ICU) and all data were extracted from the Medical Information Mart for Intensive Care III (MIMIC-III) database. The main exposure was BMI classified into normal group (18.5≤ BMI＜25), excessive group (BMI≥ 25). The outcome concluded 30-day, 90-day and 1-year mortality. The association between two BMI groups and mortality was elucidated by Cox regression models, propensity score matching (PSM) and inverse probability of treatment weighting (IPTW). The underlying effect of blood glucose on the “obesity paradox” was analyzed by causal mediation analysis. Results: A total of 522 patients were included in our study, of which 177 were the normal group and 345 were the excessive group. According to Cox regression models, a significant beneficial effect of excessive BMI in terms of mortality was observed: 30-day mortality (HR 0.57, 95%CI 0.35-0.90, p = 0.017), 90-day mortality (HR 0.53, 95%CI 0.36-0.78, p = 0.001) and 1-year mortality (HR 0.65, 95%CI 0.46-0.91, p = 0.013). The conclusions were stable after propensity score matching and inverse probability of treatment weighting. There were no interactions between BMI, gender, age, and diabetes. The causal mediation analysis showed that the increased glucose level would reduce the protective effect of excessive BMI on 30-day mortality.Conclusions: In severe stroke patients, those with excessive BMI are linked to lower mortality, while the protective effect on 30-day mortality weakened accompanied by the acute increase of glucose level.
Spectral indicators as a tool to assess the moisture status of forest habitats
Authors: Młynarczyk Adam , Konatowska Monika , Królewicz Sławomir , Rutkowski Paweł , Piekarczyk Jan 
Published: January 2022

Abstract
Measurement of water content in forest habitats is considered essential in all ecological research on forests, climate change or forest management, but also in protection against floods, wildfires and soil erosion. In traditional forest habitat classification two systems of habitat conditions analysis are found: single factor or multifactor methods. Both labour-intensive and time-consuming, and therefore costly. Remote sensing methods provide a sound and low-cost alternative. The aim of the presented study was to find the relationship between the spectral indicators obtained from satellite images and the Forest Habitats Moisture Indexes used traditionally in the Polish forest habitats classification system, allowing their practical use in forest management and the protection of forest ecosystems, not only in Poland. The research area was Bory Tucholskie National Park, in which the results of habitat studies performed with the traditional approach were compared with the analysis of 191 spectral indicators calculated for Sentinel-2 image data. Normalized Difference Vegetation Index (NDVI) was considered to best reflect the moisture content in forest habitats, but also the trophic status, provided that spectral images from the full growing season, which in the study area cover the period from June to August, are taken into account. The results of the research also highlight the impact of water reservoirs on the humidity and trophicity of forest habitats, showing a decrease in the fertility of forest habitats with distance from the water reservoir.
Research trends in healthcare and hospital administration in Japan: Content analyses of article titles in the Journal of the Japan Society for Healthcare Administration
Authors: Moteki Yasutoshi 
Published: January 2022

Abstract
Background

To understand the characteristics and changes in research trends within various academic fields, research increasingly uses text mining, content analysis, or bibliometric methods. Such methods are widely used in medicine and medical policy to analyze trend changes over extended periods.
Methods

Using text-mining, this study quantitatively analyzed research trends in studies of healthcare administration in Japan, focusing on article titles over 28 years taken from the Journal of Japan Society for Healthcare Administration, known as Byon Kanri (Hospital Administration) from 1964 to 2007. Co-occurrence network and correspondence analysis were conducted to understand the changes in research interests during this period. To readily comprehend changing research trends over time in healthcare administration studies in Japan, a 10-year period division was set, resulting in three time periods. The correspondence analysis method analyzed the features and trends in research during each period.
Results

The configuration figures derived from the correspondence analysis revealed the first dimension revealed as an axis showing time transition and the second dimension as an axis showing policy/specific situations. The extracted words displayed in the configuration maps at the intersection of the two axes were the patient, survey, and evaluation. They had no distinctive features compared to the other words and were commonly used in the titles of articles in this journal, regardless of the period.
Conclusions

The text data analysis throughout the period shows the characteristics of and changes in the research trends in healthcare and hospital administration in Japan. Many points were clarified by this paper. First, the changes in the geriatric care system of public medical insurance and the related introduction of the long-term care insurance system in 2000 are also expressed in the characteristic extracted words expressed in the co-occurrence network analysis and correspondence analysis. Second, references to the role of doctors, nurses, and other health care professionals during the 14 years after the journal name change were frequent in studies, presenting extracted words such as doctors, staff, nurses, nursing, managers, roles, and relationships. The chi-square test focusing on these extracted words and the period classification confirmed a statistically significant relationship between them.
Community roots of COVID-19 infection rates between population composition and regional systems in Romania
Authors: Sandu Dumitru 
Published: January 2022

Abstract
This is an analysis of local and regional conditions favouring the COVID-19 infections in Romanian local communities, between February 2020 - April 2021. The general hypothesis that is tested here is that reported infections with the new coronavirus are higher in communities of higher direct social interactions with people from extra-local communities. These interactions are favoured by socio-human capital, the regional capital, migration abroad experience and modernity at the local level. Other factors are captured by the cultural areas as subregions of historical regions of the country. Nuclei of higher infections with COVID-19 are located in developed communities around large cities, high modernity areas, and communities of high emigration abroad. This is, to my knowledge, one of the first approaches to the topic at the local community level. Policy implications are specified in the conclusions.
Can Mood-Stabilizing Agents Increase the Activity of Complexes I and II of the Electron Transport Chain and the Level of Two Tricarboxylic Acid Cycle Enzymes in Euthymic Bipolar Patients during the Early and Late Stages of the Disease?
Authors: Cyrino Luiz Arthur Rangel , Lima Daniela Delwing-de , Rezin Gislaine Tezza , Ferreira Gabriela Kozuchovski , Ramos Silvia Aparecida , Magro Débora Delwing-Dal , Cavichioli Natalia , Ullmann Oliver Matheus , Mayer Yasmin , de Oliveira Mariana Pacheco , Silva Larissa Espindola da 
Published: January 2022

Abstract
Bipolar disorder (BD) is associated with changes in biomarkers and mood episodes, leading to neurological damage, which may reflect alterations in cognitive functions and functionality. We analyzed the effect of four biomarkers related to metabolic energy in leukocytes. Two enzymes are related to the tricarboxylic acid (TCA) cycle (citrate synthase and succinate dehydrogenase), and two proteins are related to electron transport chain (ETC) (complexes I and II). We studied 50 euthymic patients divided into two subgroups with 25 patients (≤ 3 years and ≥ 10 years of diagnosis, from the first episode of mania) and 25 control patients. All the patients were using mood stabilizers. There were no changes in the levels of TCA cycle enzymes in the leukocytes in any subgroup. However, complex I activity showed a significant increase in patients ≤ 3 years (461.80±162.76) and ≥ 10 years (561.21±328.13) since disease onset compared to the control group (335.39±167.64) (p<0.01). Complex II activity was significantly increased in the ≥ 10 years (3.42±1.38) group compared to the ≤ 3 years (2.66±1.17) group and the control group (2.43±0.95) (p<0.01). These results suggest that stabilizing medications improve the activity of ETC complexes and stabilize TCA cycle enzymes.