bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENINGKATAN PRODUKTIVITAS USAHA MELALUI KEGIATAN PENDAMPINGAN SOSIAL SEBAGAI RANGKAIAN PROGRAM KEWIRAUSAHAAN SOSIAL PADA KECAMATAN SUSUKAN KABUPATEN SEMARANG
Authors: Eva Purnamasari  , Ayuwardani Mellasanti 
Published: October 2022

This social mentoring activity is a part of the Social Entrepreneurship Program, a collaboration between the Semarang State Polytechnic and the Ministry of Social Affairs, especially the Directorate of Individual, Family and Institutional Social Empowerment. The activity involves lecturers and students, as well as coordinators and assistants from the ministry. Susukan District is one of the areas that is a priority assistance area in Semarang Regency. The target of the program is the Beneficiary Families (KPM), and aims to provide business assistance for KPMs so that they are able to develop their ultra-micro businesses. With this assistance, the ultra-micro business of the KPM business can develop both in terms of model, capital, assets and business turnover through mentoring in production, packaging, marketing, and also legal aspect.

Kegiatan pendampingan sosial ini merupakan rangkaian dari Program Kewirausahaan Sosial, kerjasama Politeknik Negeri Semarang dengan Kementerian Sosial khususnya Direktorat Pemberdayaan Sosial Perorangan, Keluarga dan Kelembagaan Masyarakat. Kegiatan melibatkan dosen dan mahasiswa, serta koordinator serta pendamping dari pihak kementerian. Kecamatan Susukan merupakan salah satu wilayah yang menjadi area pendampingan prioritas pada Kabupaten Semarang. Sasaran program adalah para Keluarga Penerima Manfaat (KPM), dan bertujuan untuk memberikan pendampingan usaha bagi KPM agar mereka mampu mengembangkan usaha ultra mikro yang mereka miliki. Dengan pendampingan ini usaha ultra mikro usaha KPM dapat berkembang baik secara model, modal, aset dan omset usaha melalui pendampingan sosial di bidang produksi, kemasan, pemasaran dan aspek legalitas.
Red algae industrial residues as a sustainable carbon platform for the co-production of poly-3-hydroxybutyrate and gluconic acid by Halomonas boliviensis
Authors: Bondar Maryna , Pedro Filipa , Oliveira M. Conceição , da Fonseca M. Manuela R. , Cesário M. Teresa 
Published: October 2022

Polyhydroxyalkanoate (PHA) production using halophilic bacteria has been revisited because less severe operational conditions with respect to sterility can be applied, also alleviating production costs. Halomonas boliviensis was selected because it is a moderate halophile able to grow and attain high poly-3-hydroxybutyrate (P3HB) contents under 5–45 g/L NaCl concentrations, conditions that discourage microbial contamination. Industrial residues of the red alga Gelidium corneum after agar extraction were used as sugar platform to reduce costs associated with the carbon source. These residues still comprise a high carbohydrate content (30–40% w/w) of mainly cellulose, and their hydrolysates can be used as substrates for the bioproduction of value-added products. Preliminary assays using glucose were carried out to determine the best conditions for growth and P3HB production by H. boliviensis in bioreactor fed-batch cultivations. Two strategies were addressed, namely nitrogen or phosphorus limitation, to promote polymer accumulation. Similar P3HB cell contents of 50% (gpolymer/gCDW) and yields YP3HB/glucose of 0.11–0.15 g polymer/g glucose were attained under both conditions. However, higher specific productivities were reached under P-limitation, and thus, this strategy was adopted in the subsequent study. Two organic acids, resulting from glucose metabolism, were identified to be gluconic and 2-oxoglutaric acid. Reducing the oxygen concentration in the cultivation medium to 5% sat was found to minimize organic acid production and enhance the yield of polymer on sugar to 0.20 gP3HB/gglucose. Finally, fed-batch cultivations using G. corneum hydrolysates as the only C-source achieved an overall volumetric productivity of 0.47 g/(L.h), 40% polymer accumulation, and negligible gluconic acid production.
Enhanced thermal and photo-stability of a para-substituted dicumyl ketone intercalated in a layered double hydroxide
Authors: Costa Ana L. , Monteiro Rodrigo P. , Nunes Barradas Paulo D. , Ferreira Simone C. R. , Cunha Carla , Gomes Ana C. , Gonçalves Isabel S. , Seixas de Melo J. Sérgio , Pillinger Martyn 
Published: October 2022

A ketodiacid, 4,4′-dicarboxylate-dicumyl ketone (3), has been intercalated into a Zn, Al layered double hydroxide (LDH) by a coprecipitation synthesis strategy. The structure and chemical composition of the resultant hybrid material (LDH-KDA3) were characterized by powder X-ray diffraction (PXRD), FT-IR, FT-Raman and solid-state 13C{1H} NMR spectroscopies, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), thermogravimetric analysis (TGA), and elemental analysis (CHN). PXRD showed that the dicarboxylate guest molecules assembled into a monolayer to give a basal spacing of 18.0 Å. TGA revealed that the organic guest starts to decompose at a significantly higher temperature (ca. 330°C) than that determined for the free ketodiacid (ca. 230°C). Photochemical experiments were performed to probe the photoreactivity of the ketoacid in the crystalline state, in solution, and as a guest embedded within the photochemically-inert LDH host. Irradiation of the bulk crystalline ketoacid results in photodecarbonylation and the exclusive formation of the radical-radical combination product. Solution studies employing the standard myoglobin (Mb) assay for quantification of released CO showed that the ketoacid behaved as a photoactivatable CO-releasing molecule for transfer of CO to heme proteins, although the photoreactivity was low. No photoinduced release of CO was found for the LDH system, indicating that molecular confinement enhanced the photo-stability of the hexasubstituted ketone. To better understand the behavior of 3 under irradiation, a more comprehensive study, involving excitation of this compound in DMSO-d6 followed by 1H NMR, UV-Vis and fluorescence spectroscopy, was undertaken and further rationalized with the help of time-dependent density functional theory (TDDFT) electronic quantum calculations. The photophysical study showed the formation of a less emissive compound (or compounds). New signals in the 1H NMR spectra were attributed to photoproducts obtained via Norrish type I α-cleavage decarbonylation and Norrish type II (followed by CH3 migration) pathways. TDDFT calculations predicted that the formation of a keto-enol system (via a CH3 migration step in the type II pathway) was highly favorable and consistent with the observed spectral data.
Proteomics analysis of cancer tissues identifies IGF2R as a potential therapeutic target in laryngeal carcinoma
Authors: Liu Bing , Hu Yuqiang , Wan Lixia , Wang Luan , Cheng Liangjun , Sun Hai , Liu Yaran , Wu Di , Zhu Jiefei , Hong Xiu , Li Yang , Zhou Chong 
Published: October 2022

BackgroundLaryngeal cancer (LC) is a prevalent head and neck malignancy; however, the essential pathophysiological mechanism underlying its tumorigenesis and progression remains elusive. Due to the perduring scarcity of effective targeted drugs for laryngeal cancer, insights into the disease’s pathophysiological mechanisms would substantially impact the treatment landscape of laryngeal cancer.MethodsTo ensure quality consistency, 10 tumor and 9 non-tumor samples underwent proteomic analysis on a single mass spectrometer using a label-free technique. Subsequently, gene expression variations between laryngeal squamous cell carcinoma and normal tissues were analyzed using The Cancer Genome Atlas (TCGA) database. Immunohistochemical expressions of insulin-like growth factor 2 receptor (IGF2R), fibronectin (FN), vimentin, and α-smooth muscle actin (SMA) in LC tissues and normal tissues were determined.ResultsIn the tumor group, significant variations were detected for 433 upregulated and 61 downregulated proteins. Moreover, the heatmap revealed that the expressions of RNA translation-related proteins and proteins involved in RNA metabolism, such as IGF2R, tenascin C (TNC), periostin (POSTN), proteasome 26S subunit ATPase 4 (PSMC4), serpin family A member 3 (SERPINA3), heat shock protein family B (small) member 6 (HSPB6), osteoglycin (OGN), chaperonin containing TCP1 subunit 6A (CCT6A), and chaperonin containing TCP1 subunit 6B (CCT6B), were prominently elevated in the tumor group. Nonsense-mediated RNA decay (NMD), RNA translation, and protein stability were significantly altered in LC tumors. IGF2R was remarkably upregulated in LC tumors. In the TCGA database, the IGF2R mRNA level was significantly upregulated in LSCC tissues. Additionally, IGF2R mRNA expression was lowest in clinical grade 1 samples, with no significant difference between grades 2 and 3. In LSCC patients, a significant positive correlation between IGF2R expression and the stromal score was detected using the ESTIMATE algorithm to estimate the immune score, stromal score, and tumor purity in the tumor microenvironment. Lastly, immunohistochemical analysis revealed that IGF2R is overexpressed in LC.ConclusionThese results demonstrate the vital role of IGF2R in LC carcinogenesis and progression and may facilitate the identification of new therapeutic targets for the prevention and treatment of LC.
Obesity paradox and aging: Visceral Adiposity Index and all-cause mortality in older individuals: A prospective cohort study
Authors: Wang Lei , Yi Zhong 
Published: October 2022

BackgroundThe relationship between body mass index (BMI) and mortality in older adults diminished. It is necessary to examine other factors that may accurately predict mortality in older adults. The visceral adiposity index (VAI) is an uncomplicated marker specific to the gender that incorporates anthropometric data and lipid profiles. VAI has been proposed as a marker of visceral adipose tissue dysfunction and of the related cardiometabolic risk. The aim of this study was to evaluate the link of VAI with all-cause mortality among the elderly.MethodsThe present prospective cohort study included data from 1999 to 2014 provided by the National Health and Nutrition Examination Survey (NHANES) in the United States. NHANES participants at or above the age of 65 were included. Data collection was carried out by taking face-to-face interviews, mobile-physical examinations, and lab tests. From the start of the survey to the end of December 2015, mortality-related follow-up statistics are available. The shape of the link between VAI and all-cause mortality was investigated using a restricted cubic spline model. Univariate- and multivariate-adjusted Cox proportional hazard models were estimated for VAI, and the results were presented as regression coefficients and 95% confidence intervals (CI).ResultsThe 82,091 NHANES participants represented 442.2 million non-institutionalized residents of the United States. A total of 11,173 older individuals (representing 23.3 million; aged 73.4 ± 5.8 years; 56.3% women, 82.7% non-Hispanic Whites, 6.8% non-Hispanic Blacks, and 3.3% Mexican Americans) were included in the study. During the 80-month follow-up period, 4466 fatalities were reported, including 825 deaths from cancer, 867 from heart disease, and 211 from cerebrovascular disease. The restricted cubic spline model demonstrated a robust J-shaped link between VAI and all-cause mortality, revealing a significant decrease in risk within the lower range of VAI, which attained the lowest risk close to 1.7. With VAI greater than 1.7, the risk of mortality increased with the increase of VAI (P for non-linearity = 0.025). In the multivariate-adjusted model, the risk of all-cause mortality was 0.73 (0.56-0.97) and 1.05 (1.01-1.09) in participants with VAI less than 1.7 and VAI greater than or equal to 1.7, respectively.ConclusionThis investigation is a population-based cohort study with high sample sizes and a long-term in older individuals follow-up that showed a J-shaped link between VAI levels and all-cause mortality. Understanding the independent roles of VAI in the relationship between BMI and mortality is crucial to understanding the obesity paradox phenomenon.
Sediment oxygen demand rate in a flow regulated lagoon (Venice, Italy)
Authors: Leoni Simone , Dominik Janusz , Cassin Daniele , Manfè Giorgia , Tagliapietra Davide , Acri Francesco , Zonta Roberto 
Published: October 2022

From October 2020, the MOSE system went into operation in the three inlets to protect Venice and its lagoon from flooding caused by high tide events in the Adriatic Sea. While the MOSE increases the prospect of physical defense, how will the new status of a regulated lagoon affect the functioning of the waterbody? In particular, the dissolved oxygen balance in the water column can be affected by the expected increase in water residence times. Sediments play an important role in oxygen consumption in the water column and for this reason sediment oxygen demand (SOD) was studied in situ for the first time, in 16 sites at four lagoon areas. SOD rate, measured throughout 2021 with specially made benthic chambers, varied in the different areas and with seasons (from 0.63 to 24.00 g m−2 d−1), with higher values at the more confined sites and significantly lower values in those belonging to the open lagoon. Based on SOD rate, consumption times of dissolved oxygen in the water column were estimated at the MOSE closures, from saturation values to hypoxia and in the temperature range of 11.7–33.3°C. Especially in the confined sites, sediments can exert an important depleting action on dissolved oxygen already at water temperatures above 20°C, which are not particularly high for the lagoon.
Inappropriateness of space-for-time and variability-for-time approaches to infer future dryland productivity changes
Authors: Zhan Weiwei , Lian Xu , Liu Jiangong , Gentine Pierre 
Published: October 2022

Drylands are among the most susceptible ecosystems to global climate change. It has been suggested that a future surface drying trend would largely reduce gross primary productivity (GPP) in drylands, given that water is the dominant factor controlling the spatial distributions (i.e., space-for-time analogy) and inter-annual fluctuations (i.e., variability-for-time analogy) of dryland GPP. However, whether these approaches using spatial and inter-annual diagnostics are valid to infer long-term dryland GPP remains unknown. In this study, we evaluate whether space-for-time and variability-for-time approaches, which are based on the empirical scaling between GPP and dryness, are able to capture future changes in dryland GPP as simulated by 18 Earth system models (ESMs). Using observational data during 1958–2014, we identify a strong coupling between dryland GPP and the annual aridity index (AI, the ratio of precipitation to potential evapotranspiration) over both spatial and inter-annual scales. This GPP-AI scaling is used to predict future GPP changes throughout the 21st Century based on the future AI changes projected by ESMs. The space-for-time, and variability-for-time approaches predict an overall decrease of dryland GPP by -23.66 ± 10.93 (mean ±1 standard deviation) and -3.86 ± 2.22 gC m−2 yr−1, respectively, in response to future surface drying, however, the ESM projections exhibit a strong dryland GPP increase (+81.42 ± 36.82 gC m−2 yr−1). This inconsistency is because the space- and variability-based approaches, which rely on the spatial or short-term GPP-AI relationships, cannot capture the slowly-evolving but key determinant of dryland GPP changes over multi-decadal or longer timescales, which, in ESMs, is the ecosystem physiological response to rising CO2. Our study questions the validity of “the drier the less productive” hypothesis rooted in the space-for-time and variability-for-time theories, and the implementation of such theories to constrain future ecosystem changes.
Interactive effects of benthivorous fish disturbance and ammonium loading on two submersed macrophytes of contrasting growth forms based on a mesocosm study
Authors: Yu Qing , Wang Haijun , Liu Miao , Xu Chao , Ma Yu , Guo Weihua , Jeppesen Erik 
Published: October 2022

Benthivorous fish disturbance and nitrogen loading are two important factors that influence the community structure of submersed macrophytes, but their interactive effect is not well elucidated. We conducted an outdoor mesocosm experiment to examine the individual and combined effects of these two factors on the growth of two submersed macrophytes of different growth forms, i.e., the rosette-forming Vallisneria natans and the canopy-forming Myriophyllum spicatum. The treatments involved two levels of fish (Carassius auratus) disturbance crossed with two levels (0 and 12 g NH4Cl per month) of ammonium (NH4+-N) loading. For M. spicatum, we found that maximum height (MH) was reduced by 30.7%, 26.4%, and 51.0% in fish addition alone (F) and nitrogen addition treatments (N, F + N), respectively, compared with the control (C) treatment. The density of M. spicatum declined by 20%, 62% and 68.8% in the F, N and F + N treatment. The above-ground biomass (AGB) of M. spicatum respectively reduced by 56.7%, 94%, and 96.5% in the F, N and F + N treatments, and the roots/shoots ratio (R/S) increased by 114%, and 176% in N and N + F treatments, respectively. Regarding V. natans, only the MH in the N treatment was reduced (71.9%), and the density exhibited a reduction of 59.1% and 64.5% in the N and F + N treatments, respectively. The AGB of V. natans was significantly lower in the N (90.3%) and N + F (78.4%) treatments compared with the C treatment, while increased by 60.3% in F treatment. The R/S of V. natans increased by 227%, and 74.4% in the N and F + N treatments compared with the C treatment. The interactive effect of fish disturbance and high N on MH and AGB of V. natans and density of M. spicatum were antagonistic. However, the interactive effect on density and BGB of V. natans and AGB of M. spicatum were negatively synergistic. Moreover, fish activity significantly increased the concentration of total suspended solids (TSS) in the water, while total nitrogen (TN), ammonium, total phosphorus (TP), light, pH and salinity were unaffected. When fish and nitrogen were combined, TN, TP, TSS and salinity increased significantly, while pH decreased. Our study reveals that the interactive effects of fish disturbance and high N are synergistic and/or antagonistic, suggesting that the same stressor interaction may vary from synergistic to antagonistic depending on the response variables and growth forms of the macrophytes examined. Our study contributes to the understanding of how different factors can interact with each other and affect submersed macrophytes in aquatic ecosystems. This is timely and relevant knowledge, considering the range of multiple stressors involved in the decline of aquatic ecosystems worldwide at present.
Nitrogen deposition may increase litter accumulative CO2 release in a subtropical estuarine marsh
Authors: Hu Weifang , Zeng Congsheng , Tong Chuan , Li Guoliang , Lan Xue , Zhou Jiacong , Zhang Meiying , Chen Yuehmin , Zhang Linhai 
Published: October 2022

Microbial evolution-mediated CO2 from litter has aroused widespread concern, and knowing the factors controlling litter-derived CO2 is important when considering the effects of accumulative CO2 release from litter on the global greenhouse. We conducted a short-term N addition (6, 16, and 24 g N m‒2 yr‒1) experiment in Cyperus malaccensis var. brevifolius (shichito matgrass) litter decomosition. Phospholipid fatty acid (PLFA) method and enzyme method were used to analysis litter microbial community composition and enzymatic activity. During a 220-day decomposition period, there was little effect of the N amendments on litter CO2 evolution rates (9.97‒307.54 μg C g−1 h−1) with a notable exception regarding the increase of the high-N treatment at day 20. The accumulative CO2 release significantly increased after N addition in the medium and late phases. The facilitation effect on accumulative CO2 release by N amendments was more and more obvious over the decomposition time, especially for the low- and intermediate-N treatments. At the end of our experiment, compared with the control treatment, accumulative CO2 release increased 69.75%, 76.62%, and 39.93% for low-, intermediate-, and high-N treatments, respectively. These observations highlight that N deposition could cause high losses of litter C as CO2.
Promising CoSe2-CNT composite catalyst for efficient photoelectrochemical hydrogen evolution reaction
Authors: Pi Zhi-Gang , Ye Hongfeng , Han Zuqi , Yu Peng , Yin Zhuoxun , Ma Xinzhi 
Published: October 2022

CoSe2 is a typical transition metal chalcogenide and an hydrogen evolution reaction hydrogen evolution catalyst due to its efficient and inexpensive characteristics. In this work, CoSe2/CNT composite was synthesized through a one-step solvothermal method. Specifically, a current density of 10 mA cm−2 can be achieved under an overpotential of 163 mV, and the Tafel slope is 69.4 mV dec−1. The reasons for these excellent performances are: 1) the abundant catalytic active sites of CoSe2; 2) the high conductivity of CNTs; and 3) the synergistic effect between CoSe2 and CNTs; iv the solar irradiation excites electrons to join the reaction. This work proposes a new way to reasonably design the high-efficiency and low-cost transition metal chalcogenide hydrogen evolution catalysts.