bluuebunny/crossref_metadata_2025_split huggingface dataset:
E3 ubiquitin ligase MID1 ubiquitinates and degrades type‐I interferon receptor 2
Authors: Chen Xiangjie , Zhao Qian , Xu Ying , Wu Qiuyu , Zhang Renxia , Du Qian , Miao Ying , Zuo Yibo , Zhang Hong‐Guang , Huang Fan , Ren Tengfei , He Jiuyi , Qiao Caixia , Li Yue , Li Shifeng , Xu Yang , Wu Depei , Yu Zhengyuan , Lv Haitao , Wang Jun , Zheng Hui , Yuan Yukang 
Published: July 2022

AbstractType I interferon (IFN‐I) is a common biological molecule used for the treatment of viral diseases. However, the clinical antiviral efficacy of IFN‐I needs to be greatly improved. In this study, IFN‐I receptor 2 (IFNAR2) was revealed to undergo degradation at the protein level in cells treated with IFN‐I for long periods of time. Further studies found a physical interaction between the E3 ubiquitin ligase midline‐1 (MID1) and IFNAR2. As a consequence, MID1 induced both K48‐ and K63‐linked polyubiquitination of IFNAR2, which promoted IFNAR2 protein degradation in a lysosome‐dependent manner. Conversely, knockdown of MID1 largely restricted IFN‐I‐induced degradation of IFNAR2. Importantly, MID1 regulated the strength of IFN‐I signalling and IFN‐I‐induced antiviral activity. These findings reveal a regulatory mechanism of IFNAR2 ubiquitination and protein stability in IFN‐I signalling, which could provide a potential target for improving the antiviral efficacy of IFN‐I.
Mental health nurses' resilience in the context of emotional labour: An interpretive qualitative study
Authors: Delgado Cynthia , Evans Alicia , Roche Michael , Foster Kim 
Published: July 2022

AbstractStrengthening mental health nurses' (MHNs) resilience may help mitigate the negative effects of the emotional labour (EL) of their work. There is no prior evidence on MHNs' experiences of resilience in the context of EL. This interpretive qualitative study sought to explore how MHNs build and maintain their resilience in the face of high levels of EL. Semi‐structured interviews were conducted with 11 MHNs. Reflexive thematic analysis was used to analyse the data. Four main themes were constructed. The first three; Being attuned to self and others, Having a positive mindset grounded in purpose, and Maintaining psychological equilibrium through proactive self‐care, describe how MHNs build and maintain their resilience. The fourth theme, Running on emotionally empty, describes what impedes MHNs' resilience. MHNs engaged in internal self‐regulatory processes to manage their mental and emotional state. They maintained intra‐ and inter‐personal boundaries and proactively used self‐care strategies to maintain their well‐being. Through this, they were able to replenish and sustain the energy required to maintain a state of equilibrium between themselves, their interpersonal practice, and their working environment, and to positively adapt to EL. However, lack of organizational support and high workplace demands can negatively impact MHNs' equilibrium and adaptive ability. There is a need for organizations to proactively work to reduce workplace stressors, and support MHNs' professional well‐being and practice. Education and support strategies focused on strengthening MHNs' resilience, well‐being, and mental health practice capabilities, including the provision of clinical supervision, and clear role expectations within MHNs' scope of practice are recommended.
Brazing SiC ceramic to Al0.3CoCrFeNi high‐entropy alloy using Ag–Cu filler metal
Authors: Sun Jie , Lei Yu , Fu Wei , Lin Danyang , Hu Shengpeng , Song Xiaoguo , Cao Jian , Yang Minxuan 
Published: July 2022

AbstractRealizing the joining of SiC ceramic to Al0.3CoCrFeNi high‐entropy alloy is a technical interest for aviation, aerospace, and nuclear energy industry. In this study, the reliable brazing of SiC and Al0.3CoCrFeNi high‐entropy alloy was realized by inactive AgCu filler, which lays the foundation for the following research on ceramic‐high‐entropy alloy dissimilar joining. The interfacial microstructure was characterized by a scanning electron microscope and transmission electron microscope in detail, and the influence of Al0.3CoCrFeNi dissolution on interfacial reactions was studied. The effect of brazing temperature on microstructure evolution and mechanical properties was discussed. The results indicated that elements Ni, Fe, Co, and Cr from Al0.3CoCrFeNi were crucial for the joint formation. (Ni, Fe, Co)2Si + graphite + Cr23C6/(Cr, Fe)23C6 + (Ni, Fe, Co)2Si formed adjacent to SiC and (Fe, Co, Cr, Ni)–Si silicide + Cu(s,s) + Cu–Ni–Al‐rich phase formed next to Al0.3CoCrFeNi. The reaction layers on SiC side thickened and the morphology of (Fe, Co, Cr, Ni)–Si‐ and Cu–Ni–Al‐rich phase experienced distinct changes with the increasing temperature. The highest strength of ∼32.2 MPa was obtained at 850°C for 10 min.
Aquatic environments: A potential source of antimicrobial-resistant Vibrio spp.
Authors: Kumarage Prasanga Madhushani , De Silva Liyana Arachchilage Dinithi Sandunika , Heo Gang-Joon 
Published: July 2022

Abstract
Vibrio spp. are associated with water and seafood-related outbreaks worldwide. They are naturally present in aquatic environments such as seawater, brackish water and freshwater environments. These aquatic environments serve as the main reservoirs of antimicrobial-resistant genes and promote the transfer of antimicrobial-resistant bacterial species to aquatic animals and humans through the aquatic food chain. Vibrio spp. are known as etiological agents of cholera and non-cholera Vibrio infections in humans and animals. Antimicrobial-resistant Vibrio species have become a huge threat in regard to treating Vibrio infections in aquaculture and public health. Most of the Vibrio spp. possess resistance towards the commonly used antimicrobials, including β-lactams, aminoglycosides, tetracyclines, sulphonamides, quinolones and macrolides. The aim of this review is to summarize the antimicrobial resistance properties of Vibrio spp. isolated from aquatic environments to provide awareness about potential health risks related to Vibrio infections in aquaculture and public health.
Development and application of CRISPR-based genetic tools in Bacillus species and Bacillus phages
Authors: Song Yafeng , He Siqi , Jopkiewicz Anita , Setroikromo Rita , van Merkerk Ronald , Quax Wim J. 
Published: July 2022

Abstract
Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been developed into a precise and efficient genome editing tool. Since its discovery as an adaptive immune system in prokaryotes, it has been applied in many different research fields including biotechnology and medical sciences. The high demand for rapid, highly efficient and versatile genetic tools to thrive in bacteria-based cell factories accelerates this process. This review mainly focuses on significant advancements of the CRISPR system in Bacillus subtilis, including the achievements in gene editing, and on problems still remaining. Next, we comprehensively summarize this genetic tool's up-to-date development and utilization in other Bacillus species, including B. licheniformis, B. methanolicus, B. anthracis, B. cereus, B. smithii and B. thuringiensis. Furthermore, we describe the current application of CRISPR tools in phages to increase Bacillus hosts' resistance to virulent phages and phage genetic modification. Finally, we suggest potential strategies to further improve this advanced technique and provide insights into future directions of CRISPR technologies for rendering Bacillus species cell factories more effective and more powerful.
Effects of Scoparone on differentiation, adhesion, migration, autophagy and mineralization through the osteogenic signalling pathways
Authors: Park Kyung‐Ran , Kim Bomi , Lee Joon Yeop , Moon Ho‐Jin , Kwon Il Keun , Yun Hyung‐Mun 
Published: July 2022

AbstractScoparone (SCOP), an active and efficient coumarin compound derived from Artemisia capillaris Thunb, has been used as a traditional Chinese herbal medicine. Herein, we investigated the effects of SCOP on the osteogenic processes using MC3T3‐E1 pre‐osteoblasts in in vitro cell systems. SCOP (C11H10O4, > 99.17%) was purified and identified from A. capillaries. SCOP (0.1 to 100 μM concentrations) did not have cytotoxic effects in pre‐osteoblasts; however, it promoted alkaline phosphatase (ALP) staining and activity, and mineralized nodule formation under early and late osteogenic induction. SCOP elevated osteogenic signals through the bone morphogenetic protein 2 (BMP2)‐Smad1/5/8 pathway, leading to the increased expression of runt‐related transcription factor 2 (RUNX2) with its target protein, matrix metallopeptidase 13 (MMP13). SCOP also induced the non‐canonical BMP2‐MAPKs pathway, but not the Wnt3a‐β‐catenin pathway. Moreover, SCOP promoted autophagy, migration and adhesion under the osteogenic induction. Overall, the findings of this study demonstrated that SCOP has osteogenic effects associated with cell differentiation, adhesion, migration, autophagy and mineralization.
Effect of encapsulation and natural polyphenolic compounds on bacteriophage stability and activity on Escherichia coli in Lactuca sativa L. var. longifolia
Authors: Pacios‐Michelena Sandra , Rodríguez‐Herrera Raúl , Rincón‐Enríquez Gabriel , Ramos‐González Rodolfo , Flores‐Gallegos Adriana C. , Chávez‐González Mónica L. , de la Peña González Jonathan Enmanuel , Ilina Anna 
Published: July 2022

AbstractThe goals of the present study were to characterize the phages isolated by magnetic nanocomplex, to determine the effect of natural polyphenols on phage activity against E. coli and the stability of free and encapsulated bacteriophages under UV irradiation, and their efficacy against E. coli on romaine lettuce (Lactuca sativa L. var. longifolia) as a fresh food model. The morphology of the two magnetically isolated phages were characterized using the transmission electron microscope (TEM). All isolated phages were classified in a morphological family of tailed phages of the order Caudovirales, Myoviridae family. The PCR results showed that the stx1 and cI genes were not on any phage. Assays were carried out to determine the effect of four polyphenolic natural extracts on phages under UV radiation. The highest activity and stability of phages expressed in phage titer (PFU/ml) were observed in the presence of polyphenols from tarbush leaves (Flourensia cernua DC). Coliphages were encapsulated in alginate/chitosan and alginate/PVA carriers with and without polyphenols from tarbush leaves. Encapsulation led to a higher phage stability under UV radiation. All encapsulated formulations and free coliphages were applied on the surfaces of romaine lettuce containing E. coli cells. An effective reduction of 2 log of E. coli titers from lettuce was observed. Encapsulated phage formulations with and without polyphenols from tarbush leaves could be considered for future application in fresh food preservation.
Efficacy and safety of a new peeling formulated with a pool of PHAs for the treatment of all skin types, even sensitive
Authors: Gentili Giulia , Perugini Paola , Bugliaro Stefano , D'Antonio Camilla 
Published: July 2022

AbstractBackgroundActually, the use of chemical peels in cosmetics and dermatology continues to grow due to their versatility, clinical endpoint–directed predictability, and favorable risk profile in comparison to lasers. The chemical peel is a generally safe method for treatment of some skin disorders and to refresh and rejuvenate the skin. The major challenge of chemical peels is the tolerability, that is because of sensitive skin which is one of the most common skin disorders.AimThe aim of this study was to evaluate the effectiveness of the new Miamo Renewal Peel Serum formulated with a pool of new generation acids (ELPA25™) on sensitive skin with respect to mandelic acid serum only and with respect to placebo comparison.Materials and MethodsThe “in vivo” study following the half‐face experimental protocol active versus placebo involved 30 healthy Caucasian female volunteers between 25 and 64 years, with sensitive skin, who were divided into two different groups. ELPA25™ serum was applied in one group three times a week for 8 weeks. The other group, with the same protocol, applied an active serum containing mandelic acid, as control, versus placebo. In particular, skin moisturizing, skin viscoelastic properties, skin surface smoothness, wrinkle reduction, and stratum corneum renewal were evaluated.ResultsRenewal Peel Serum was very well tolerated from sensitive skin. A significant decrease in skin roughness and wrinkle breadth, and an improvement in firmness and in skin elasticity, was observed after 2 months of treatment with respect both to mandelic acid serum and to placebo comparison.ConclusionsScientific protocol using self‐controlled study methodology and noninvasive skin bioengineering techniques with adequate statistical methods were able to evaluate both the safety and the efficacy of the new Miamo Renewal Peel Serum. This study highlighted that the Miamo Renewal Peel Serum formulated with a patent‐pending mixture of new generation acids (ELPA25™) exerts many beneficial effects and it can be successfully employed for sensitive skin.
The sword or the plowshare: Conflict and third‐party groups' reaction to violent versus nonviolent resistance
Authors: Ünal Helin , Adelman Levi , Leidner Bernhard 
Published: July 2022

AbstractAround the world, movements for justice or social change struggle with the question of whether to use nonviolent or violent protest strategies. While research suggests that nonviolent strategies may be more successful than violent ones, people's preferences and support for different strategies may depend on their specific role in the conflict. We tested this in Study 1 in the context of the Kurdish question in Turkey (N = 320), and we found that Turks and Americans supported nonviolent movements more than violent movements, while Kurds were equally supportive of both. Study 2 (N = 192) replicated Study 1 and investigated whether the preference for nonviolent strategies among the third‐party group was dependent on the perceivers' specific preferred outcomes in the conflict. We found that, in the context of the Kurdish question in Syria, third‐party Americans still supported nonviolent movements more than violent movements regardless of their preferred outcomes, although the more that they preferred that Kurds would win the conflict, the more supportive they were of both nonviolent and violent protest movements. These studies suggest that the preference for nonviolent strategies may depend on people's role in the conflict, with important implications for addressing conflict needs and conflict resolution.
Contact‐free radar recordings of body movement can reflect ultradian dynamics of sleep
Authors: Heglum Hanne Siri Amdahl , Drews Henning Johannes , Kallestad Håvard , Vethe Daniel , Langsrud Knut , Sand Trond , Engstrøm Morten 
Published: July 2022

SummaryThis work aimed to evaluate if a contact‐free radar sensor can be used to observe ultradian patterns in sleep physiology, by way of a data processing tool known as Locomotor Inactivity During Sleep (LIDS). LIDS was designed as a simple transformation of actigraphy recordings of wrist movement, meant to emphasise and enhance the contrast between movement and non‐movement and to reveal patterns of low residual activity during sleep that correlate with ultradian REM/NREM cycles. We adapted the LIDS transformation for a radar that detects body movements without direct contact with the subject and applied it to a dataset of simultaneous recordings with polysomnography, actigraphy, and radar from healthy young adults (n = 12, four nights of polysomnography per participant). Radar and actigraphy‐derived LIDS signals were highly correlated with each other (r > 0.84), and the LIDS signals were highly correlated with reduced‐resolution polysomnographic hypnograms (rradars >0.80, ractigraph >0.76). Single‐harmonic cosine models were fitted to LIDS signals and hypnograms; significant differences were not found between their amplitude, period, and phase parameters. Mixed model analysis revealed similar slopes of decline per cycle for radar‐LIDS, actigraphy‐LIDS, and hypnograms. Our results indicate that the LIDS technique can be adapted to work with contact‐free radar measurements of body movement; it may also be generalisable to data from other body movement sensors. This novel metric could aid in improving sleep monitoring in clinical and real‐life settings, by providing a simple and transparent way to study ultradian dynamics of sleep using nothing more than easily obtainable movement data.
Whole‐genome phylogeography of the blue‐faced honeyeater (Entomyzon cyanotis) and discovery and characterization of a neo‐Z chromosome
Authors: Burley John T. , Orzechowski Sophia C. M. , Sin Simon Yung Wa , Edwards Scott V. 
Published: July 2022

AbstractWhole‐genome surveys of genetic diversity and geographic variation often yield unexpected discoveries of novel structural variation, which long‐read DNA sequencing can help clarify. Here, we report on whole‐genome phylogeography of a bird exhibiting classic vicariant geographies across Australia and New Guinea, the blue‐faced honeyeater (Entomyzon cyanotis), and the discovery and characterization of a novel neo‐Z chromosome by long‐read sequencing. Using short‐read genome‐wide SNPs, we inferred population divergence events within E. cyanotis across the Carpentarian and other biogeographic barriers during the Pleistocene (~0.3–1.7 Ma). Evidence for introgression between nonsister populations supports a hypothesis of reticulate evolution around a triad of dynamic barriers around Pleistocene Lake Carpentaria between Australia and New Guinea. During this phylogeographic survey, we discovered a large (134 Mbp) neo‐Z chromosome and we explored its diversity, divergence and introgression landscape. We show that, as in some sylvioid passerine birds, a fusion occurred between chromosome 5 and the Z chromosome to form a neo‐Z chromosome; and in E. cyanotis, the ancestral pseudoautosomal region (PAR) appears nonrecombinant between Z and W, along with most of the fused chromosome 5. The added recombination‐suppressed portion of the neo‐Z (~37.2 Mbp) displays reduced diversity and faster population genetic differentiation compared with the ancestral‐Z. Yet, the new PAR (~17.4 Mbp) shows elevated diversity and reduced differentiation compared to autosomes, potentially resulting from introgression. In our case, long‐read sequencing helped clarify the genomic landscape of population divergence on autosomes and sex chromosomes in a species where prior knowledge of genome structure was still incomplete.
Screening of azole resistance in Aspergillus fumigatus using the EUCAST E.Def 10.2 azole‐containing agar method: A single study suggests that filtration of conidial suspensions prior to inoculum preparation may not be needed
Authors: Serrano‐Lobo Julia , Gómez Ana , Reigadas Elena , Muñoz Patricia , Escribano Pilar , Guinea Jesus ,   the ASPE IN Study Group
Published: July 2022

AbstractBackgroundAzole resistance screening in Aspergillus fumigatus isolates can be routinely carried out by using azole‐containing plates (E.Def 10.2 method), that requires filtering conidial suspensions prior inoculum adjustment.ObjectivesWe evaluated whether skipping the filtration step of conidial suspensions negatively influences the performance of the E.Def 10.2.Patients/MethodsA. fumigatus sensu stricto isolates (n = 92), classified as azole‐susceptible or azole‐resistant according to the EUCAST microdilution E.Def 9.4 method, were studied. Azole‐resistant isolates had either wild type cyp51A gene sequence (n = 3) or the TR34‐L98H (n = 26), G54R (n = 5), TR46‐Y121F‐T289A (n = 1), F46Y‐M172V‐N248T‐D255E‐E427K (n = 1), F165L (n = 1) or G448S (n = 1) cyp51A gene substitutions. In‐house azole‐containing agar plates were prepared according to the EUCAST E.Def 10.2 procedure. Conidial suspensions were obtained by adding distilled water (Tween 20 0.1%). Subsequently, the suspensions were either filtered or left unfiltered prior to inoculum adjustment to 0.5 McFarland. Using microdilution as the gold standard, agreement, sensitivity and specificity of the agar plates inoculated with two inoculums were assessed.ResultsAgreements for the agar screening method with either unfiltered or filtered conidial suspensions were high for itraconazole (100%), voriconazole (100%) and posaconazole (97.8%). Sensitivity (100%) and specificity (98.2%) of the procedure to rule in or out resistance when unfiltered suspensions were used were also high. Isolates harbouring the TR34‐L98H, G54R and TR46‐Y121F‐T289A substitutions were detected with the modified method.ConclusionsUnfiltered conidial suspensions does not negatively influence the performance of the E.Def 10.2 method when screening for A. fumigatus sensu stricto.
Laccaria bicolor pectin methylesterases are involved in ectomycorrhiza development with Populus tremula × Populus tremuloides
Authors: Chowdhury Jamil , Kemppainen Minna , Delhomme Nicolas , Shutava Iryna , Zhou Jingjing , Takahashi Junko , Pardo Alejandro G. , Lundberg‐Felten Judith 
Published: July 2022

Summary

The development of ectomycorrhizal (ECM) symbioses between soil fungi and tree roots requires modification of root cell walls. The pectin‐mediated adhesion between adjacent root cells loosens to accommodate fungal hyphae in the Hartig net, facilitating nutrient exchange between partners. We investigated the role of fungal pectin modifying enzymes in Laccaria bicolor for ECM formation with Populus tremula × Populus tremuloides.

We combine transcriptomics of cell‐wall‐related enzymes in both partners during ECM formation, immunolocalisation of pectin (Homogalacturonan, HG) epitopes in different methylesterification states, pectin methylesterase (PME) activity assays and functional analyses of transgenic L. bicolor to uncover pectin modification mechanisms and the requirement of fungal pectin methylesterases (LbPMEs) for ECM formation.

Immunolocalisation identified remodelling of pectin towards de‐esterified HG during ECM formation, which was accompanied by increased LbPME1 expression and PME activity. Overexpression or RNAi of the ECM‐induced LbPME1 in transgenic L. bicolor lines led to reduced ECM formation. Hartig Nets formed with LbPME1 RNAi lines were shallower, whereas those formed with LbPME1 overexpressors were deeper.

This suggests that LbPME1 plays a role in ECM formation potentially through HG de‐esterification, which initiates loosening of adjacent root cells to facilitate Hartig net formation.
EARLY ISLAMIC CRUDE HANDMADE WARE: NEW INSIGHTS ON THE TYPOLOGY, CHRONOLOGY AND PROVENANCE OF A SOUTHERN LEVANTINE HANDMADE POTTERY
Authors: Taxel Itamar , Shamir Orit , Waiman‐Barak Paula , Ondricek Willie , Ayalon Etan 
Published: July 2022

SummaryThis study presents new evidence about a class of Early Islamic ceramics that have been largely overlooked; namely, crude handmade ware, sometimes previously referred to as ‘Negebite’ Ware, typical of the southern portions of what is now modern‐day Israel and Jordan. A large array of handmade vessels retrieved in excavations of an Early Islamic settlement in the Yotvata oasis of the ‘Arabah Valley, Israel and comparative material from neighbouring sites, provide a basis for new perceptions. These consist of a typo‐chronology of Early Islamic crude handmade wares, clarification of previously unrecognized production techniques (including ones based on evidence from textile impressions), and the results of initial petrographic analysis. We also explore chronological and cultural links between this family of ceramics and other endemic Islamic‐period handmade wares that are known from the region.