bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis And Bio-Evaluation Of Some 4-Hydroxycoumarin Derivatives
Authors: Al-Majedy Yasameen K. Kadhim Hasan , Ibraheem Hiba H. , Shaker Samar M. 
Published: February 2022

Abstract
Coumarins are naturally occurring oxygen heterocyclic compounds having multifarious medicinal properties, hence used as lead compounds for designing new potent analogs. The modification of 4-hydroxycoumarin by different reaction steps was done to yield target compounds. The structures of the synthesized compounds were characterized by spectroscopic methods, Infrared Spectra FT-IR and 1H Nuclear Magnetic Resonance Spectra and elemental analysis techniques, melting point and thin layer chromatography. The newly synthesized derivatives contain different functional groups, and as such they could exhibit microbiological activity. Therefore, we tested the microbiological activity of these derivatives on various species of bacteria and fungi. The tested compounds have shown different activity in terms of growth inhibition of microorganisms. Newly synthesized derivatives exhibit antibacterial activities.
Inhibition of Spinal 5-HT3 Receptor and Spinal Dorsal Horn Neuronal Excitability Alleviates Hyperalgesia in A Rat Model of Parkinson's Disease
Authors: Li Cheng-Jie , Liu Lu-Bing , An Meng-Qi , Zhang Li-Ge , Dong Li-guo , Gu Han-Ying , Dai Yong-Ping , Wang Fen , Mao Cheng-Jie , Liu Chun-Feng 
Published: February 2022

Abstract
Pain in Parkinson’s disease (PD) is increasingly recognized as a major factor associated with poor health-related quality of life. However, classic therapeutic drugs supplying dopamine have limited therapeutic effect on PD related pain. This suggests that there is a mechanism outside the dopamine system that causes pain in PD. Our previous study has demonstrated that 6-OHDA induced PD model manifested hyperalgesia to thermal and mechanical stimuli and decreased serotonin (5-HT) contents in the spinal dorsal horn (SDH). Several 5-HT receptor subtypes have been confirmed to be associated with nociception in the spinal cord, such as 5-HT1A receptor, 5-HT1B receptor, 5-HT2 receptor, 5-HT3 receptor, and 5-HT7 receptor. Most researches have shown that 5-HT1A receptor and 5-HT3 receptor played a key role in pain transmission in the spinal cord. Thus, we hypothesize that hyperalgesia of 6-OHDA rats may be related to increased excitability of SDH neurons and the functional change of 5-HT3 receptor may reverse the hyperalgesia of 6-OHDA rats and decrease cell excitability of SDH neurons. To test this hypothesis, we used whole-cell patch-clamp and pharmacological methods to evaluate the effect of 5-HT3 receptor and 5-HT1A receptor on the hyperalgesia of 6-OHDA rats. The results suggested that increased excitability in SDH neurons could be reversed by 5-HT3 receptor antagonist ondansetron (20 µmol/L), but not 5-HT3 receptor agonist M-CPBG (30 µmol/L), 5-HT1A receptor antagonist 8-OH DPAT (10 µmol/L)and agonist WAY-100635 (10 µmol/L). Intrathecal injection with ondansetron (0.1 mg/kg) but not M-CPBG (0.1 mg/kg), 8-OH DPAT (0.1 mg/kg) and WAY-100635 (0.1 mg/kg) significantly attenuated the mechanical hyperalgesia and thermal hyperalgesia of 6-OHDA rats. Therefore, the present study suggests that inhibition of 5-HT3 receptor relieves hyperalgesia in PD rats by reducing the excitability of SDH neurons. Our study provides a novel mechanism or therapeutic strategy for pain in patients with PD.
Surgical Treatment on Infective Endocarditis: Impact of Diabetes on Mortality
Authors: Kogan Alexander , Wieder Anat , Frogel Jonathan , Peled-Potashnik Yael ,   Eilon                                 and Leonid Sternik1,5. Ram, Raanani Ehud , Sternik Leonid 
Published: February 2022

Abstract

BackgroundType 2 diabetes mellitus (DM) is a frequent co-morbidity among patients suffering from infective endocarditis (IE). The aim of the study was to evaluate the impact of type 2 DM on the early-, intermediate- and long-term mortality of patients who underwent surgical treatment of endocarditis.MethodsWe performed an observational cohort study in the large tertiary center in Israel during 14 years. All data of patients who underwent surgical treatment of endocarditis, performed between 2006 and 2020 were extracted from the departmental database. Patients were divided into two groups: Group I (non-diabetic patients), and Group II (diabetic patients).ResultsThe study population includes 420 patients. Group I (non-diabetic patients), comprise 326 patients, and Group II (diabetic patients), comprise 94 patients. Mean follow-up duration was 39.3 ± 28.1 months. Short-term, 30-day and in-hospital mortality, also intermediate-term mortality (1- and 3-year) was higher in the DM group compared with the non-DM group, but did not reach statistical significance: 11.7% vs. 7.7%. (p = 0.215); 12.8% vs. 8.3% (p = 0.285); 20.2% vs. 13.2% (p = 0.1) and 23.4% vs. 15.6% (p = 0.09) respectively. Long-term, 5-year mortality was significantly higher in the DM group, compared to the non-DM group: 30.9% vs. 16.6% (p = 0.003). Furthermore, predictors for long-term mortality included diabetes (CI 1.056–2.785, p = 0.029), as demonstrated by regression analysis.ConclusionsDiabetic patients have trend to increasing mortality at the short- and intermediate period post-surgery for IE, but this is not statistically significant. Survival of diabetic patients deteriorates after more than three years follow surgery. Diabetes is an independent predictor for long-term, 5-year mortality after surgical treatment of endocarditis, regardless of the patients age and comorbidities.
Application of zinc oxide reduces the bioavailability and specific uptake of cadmium by alfalfa grown in a cadmium-contaminated alkaline soil
Authors:   Honghua (S102) He, Zhang Zekun , Wu Miaomiao 
Published: February 2022

Abstract

Aims Zinc (Zn) and cadmium (Cd) have similar chemical properties and interact with each other in the soil-plant system. Application of Zn may be an effective way for phytoremediation of Cd-contaminated soils.Methods A greenhouse pot experiment was carried out to grow alfalfa in an alkaline soil spiked with different rates of Cd (0, 20, and 80 mg kg–1) and Zn (0, 200, and 800 mg kg–1) as cadmium oxide (CdO) and zinc oxide (ZnO), respectively, to investigate the effects of ZnO application on soil Cd bioavailability, plant growth and Cd uptake. Results Adding ZnO resulted in a lower soil pH and greater DTPA-extractable Zn concentration, but a lower DTPA-extractable Cd concentration. The effect of ZnO addition on Cd bioavailability depended on both soil Cd level and Zn dose. Soil Cd contamination considerably inhibited plant growth and increased both root and shoot Cd concentrations. Adding ZnO significantly enhanced plant growth and reduced specific Cd uptake by roots and plant Cd concentrations, but did not affect the total amount of Cd taken up by plants. The percentage of total plant Cd content allocated to roots was significantly lower, but the root-to-shoot translocation factor of Cd was higher when ZnO was added. Both CdO and ZnO addition had a small effect on root exudation of carboxylates. Conclusions Application of ZnO to soil alleviated Zn deficiency and reduced Cd bioavailability, consequently enhanced plant growth and Cd concentrations, and it may be a cost-effective way for phytoremediation of Cd-contaminated soils.
Effect of Autologous Bone Marrow Derived Mesenchymal Stem Cells in Treatment of Rheumatoid Arthritis.
Authors: Elbasha Yara I. , El-Maaty Dina M. Abo , Mesbah Noha M. , Ramadan Asmaa M. , Bakry Sayed M. , Mansour Ahmad M. , Elbeialy Adel A. 
Published: February 2022

Abstract
Background
Chronic inflammation causes articular bone and cartilage degeneration in people with rheumatoid arthritis (RA). Despite recent advances, management of RA is frequently accompanied with negative consequences, and ineffectiveness. Access to treatment is still hampered by many factors including financial restrictions. As a result, medications that reduce inflammation and bone resorption that are both safer and less expensive are required. Mesenchymal stem cells have recently been identified as a potential therapy for rheumatoid arthritis.
Aim of Study:
The aim of this study was to examine the anti-arthritic effect of rat bone marrow-MSCs, oligosaccharides and human placental extract, individually and combined on RA model, using Complete Freund's adjuvant (CFA) induced arthritis in rats.
Materials and Methods
In female rats, rheumatoid arthritis was induced by injecting Complete Freund's adjuvant in the paw of hind limbs. Treatments with rat bone marrow-mesenchymal stem cells via intravenous route, oligosaccharides, and human placental extract were given individually and in combination via intra-peritoneal route. Measurement of complete blood picture (CBC), ESR, cortisol, urea, uric acid, liver and renal biochemical parameters were carried out to determine the safety and efficacy of the different combinations. Inflammatory cytokines TNF- α, IL-6 and IL-10 were measured to assess anti-inflammatory effects. Histopathological analysis of bone sections was carried out too.
Results
Our findings showed that adding innovative treatments involving oligosaccharides and human placental extract to rat-bone marrow mesenchymal stem cells in a CFA-induced arthritic rat model resulted in a favorable anti-arthritic inflammatory effect as shown in reduction of inflammatory cytokines, with complete safety on blood picture, renal and liver functions, cortisol, and ESR. Histopathological analysis went concurrent with these results showing healing and remodeling of osteoporotic lesions induced in arthritic rat model.
Conclusions
The combination of rBM-MSCs via intravenous route, with oligosaccharides, and human placental extract could be employed as a powerful therapeutic medication to treat rheumatoid arthritis.
Antiviral efficacy of cerium oxide nanoparticles
Authors: Nefedova Alexandra , Rausalu Kai , Zusinaite Eva , Vanetsev Alexander , Rosenberg Merilin , Koppel Kairi , Lilla Stevin , Visnapuu Meeri , Šmits Krišjānis , Kisand Vambola , Tätte Tanel , Ivask Angela 
Published: February 2022

Abstract
Nanomaterials have been proposed as good candidates for the elimination of viruses due to their multimodal mechanisms of action. Here, we tested the antiviral potential of cerium dioxide (CeO2) nanoparticles which is largely unexplored.Two types of nano-CeO2 particles with opposing surface charge, nano-CeO2(+) and nano-CeO2(-), were assessed for their capability to decrease the plaque forming units (PFU) of four enveloped and two non-enveloped viruses during 1 h exposure. Statistically significant antiviral activity of nano-CeO2 towards enveloped coronavirus SARS-CoV-2 and influenza virus A/WSN/1933 was registered already at 20 mg Ce/l. Significant decrease in PFU of other two enveloped viruses, transmissible gastroenteritis virus TGEV and bacteriophage φ6 was evidenced at 200 mg Ce/l. For all the enveloped viruses the maximum reduction of PFU after 1 h exposure to nano-CeO2 exceeded 2 logs, that has been considered as the lowest biologically meaningful activity in antiviral applications. For most of the enveloped viruses, 1 h exposure to nano-CeO2 resulted in ≥ 4 log reduction in PFU. As expected, the sensitivity of non-enveloped viruses towards nano-CeO2 was significantly lower than that of enveloped viruses. Until the highest tested concentration, 2000 mg Ce/l neither of the nano-CeO2 showed an effect on picornavirus EMCV and only nano-CeO2(-) caused a slight non-monotonic response in MS2 bacteriophage.Parallel testing of antiviral activity of Ce3+ ions and SiO2 nanoparticles allows to conclude that nano-CeO2 activity was due to neither released Ce3+ ions nor nonspecific effects of any nanosized particulate. Interestingly, we did not evidence any significant antiviral activity of Ag nanoparticles at 1 h exposure. This result referred to notably higher antiviral activity of nano-CeO2 compared with nanosilver that has been generally considered as active against viruses. Although exhibiting antiviral effects, the antibacterial activity of nano-CeO2 was very low. These results along with non-existent cytotoxicity nano-CeO2 allow us to propose nano-CeO2 for specific antiviral applications.
Transfusion of older red blood cells increase the risk of postoperative infectious complications in pediatric scoliosis surgery: a propensity score matched analysis
Authors: Wang Lipeng , Liu Jiangli , Chu Shuaishuai , Cui Ying , Ma Zhengliang , Xia Tianjiao , Gu Xiaoping , Chen Yongquan 
Published: February 2022

Abstract
Purpose: Pediatric scoliosis patients undergoing posterior spinal fusion surgery often need RBCs transfusion. However, the effects of storage time of transfused blood on perioperative outcomes remain undefined.Methods: A retrospective study including 539 pediatric patients undergoing posterior spinal fusion surgery was conducted. These patients were divided into 2 groups: the fresh group, who received exclusively blood cells stored for 14 days or less; the older group, who received exclusively blood for more than 14 days. The effect of red blood cell storage time on length of hospital stay and postoperative complications was analyed by propensity score matching. Results: The propensity score matching yielded 78 well-matched pairs after adjusting for potential confounders in baseline characteristics. Patients who received older blood had not prolonged length of stay compared with those received fresh blood (13.0 ± 3.8 days vs 11.8 ± 2.6 days, p = 0.09). However, multivariable logistic regression analysis showed that older group had a higher incidence of infectious complications compared with fresh group (OR = 2.15 [95% CI, 1.08–4.31]; P = 0.029). Conclusions: In pediatric scoliosis patients undergoing posterior spinal fusion surgery, transfusion of older RBCs had a higher incidence of postoperative infectious complications but was not associated with prolonged length of hospital stay.
Anatomical Dissection as a Source of Clinically Relevant Findings with Potential Medico-Legal Implications
Authors: Dzetkuličová Veronika , Handlos Petr , Vojtíšek Tomáš , Joukal Marek 
Published: February 2022

Abstract
PurposeBodies bequeathed to anatomical departments of medical schools in the Czech Republic for anatomical teaching and research belong to donors whose manner of death is deemed natural and does not require further investigation with medico-legal autopsies. However, there are rare cases when critical medico-legal findings related to the cause/manner of death or clinically relevant past surgical procedures can be found during anatomical dissection. This article also adds to the wealth of literature supporting the argument that pathologies and clinically relevant findings encountered by medical students during anatomical dissection are an opportunity for problem-based learning and integration of clinically oriented modules into early undergraduate phases of medical degree curricula.MethodsThis article gives an example of an extrapericardial iatrogenic diaphragmatic hernia which was most likely caused by instrument manipulation during a thoracic surgical procedure and went unobserved for three years until the body was subject to anatomical dissection. ResultsAlthough this clinical finding was most likely unconnected to the cause of death of the donor, the case is a cautionary example that bodies undergoing anatomical dissection could contain evidence of previous surgical incaution during invasive procedures. ConclusionsFor the benefit of anatomical technicians and educators, this article also points out some examples when signs of suspicious death could be overlooked by medical professionals who establish a cause of death in bodies which could be a subject to body fixation and subsequent anatomical dissection.
Adolescent Electronic Cigarette Use: A Bibliometric Analysis of Global Scientific Research
Authors: Li Jin , Wang Chen , Wang Xiangyu , Duan Zhiguang 
Published: February 2022

Abstract
Background
Adolescent electronic cigarette (e-cigarette) use is an emerging global public health concern as nicotine addiction may be established among this vulnerable population. Its study has generated a burgeoning body of research charactering a wide spectrum. However, the development and current status of research on this alarming issue have been inadequately examined in an objective, systematic and visualized way. This bibliometric analysis aims to fill this gap by providing a comprehensive overview of scientific literature in relation to adolescent e-cigarette use.
Methods
A total of 943 eligible papers published from 2011 to 2021 were retrieved from Web of Science Core Collection. Performance analysis was used with various bibliometric indicators to examine the publication trend, most prolific countries, institutions, authors, journals, and highly cited articles. Science mapping based on VOSviewer was employed to visualize the collaboration networks between countries and authors, research hotspots, and their evolutions.
Results
Publications on adolescent e-cigarette use has increased significantly during the past decade. The USA dominated this field in all aspects and the University of Southern California was the most productive institution. There were clear collaborative author groupings, but their transnational collaborations were generally weak. Major research themes were identified in the extant literature, including epidemiology and KABs; risk factors; health implications; gateway effect; and prevention interventions. The keywords such as ‘vapor’, ‘flavor’, ‘advertising’, ‘dual use’ reflected research emphases in early studies while ‘bullying’, ‘COVID-19’, ‘social norms’, and ‘heated tobacco products’ were the emerging research trends.
Conclusion
This study is presumed to provide an overall picture of research on adolescent e-cigarette use, which may help scholars and public health practitioners stay abreast of the research developments and serve as a reference and entry point for further investigation into this multifaceted phenomenon.
Anatomo-radyographic study in individuals with and without medial patellofemoral ligament rupture
Authors: KABAKCI Ayse Gul , Ögüt Emre , Tekbaş Volkan Tolga , Bozkır Memduha Gülhal 
Published: February 2022

Abstract
Purpose; The purpose of the present study was to evaluate of patella elevation (patellar height) and trochlear groove angle in individuals with and without medial patellofemoral ligament rupture for surgical reconstruction and possible clinical use.Materials and Methods; The study had a retrospective design, and included 149 people (53 females, 96 men) who were between the ages of 18 and 36 admitting to the Orthopedic Clinic of Bartın State Hospital with various complaints in knee joints and different preliminary diagnoses between January 2005 and December 2020. 61 of the 149 people in the study were patients with medial patellofemoral rupture. Two groups were examined, those with and without medial patellofemoral rupture.Results; As a result of the study, while significant differences were detected in trochlear groove angle, there wasn’t significant differences in patella elevation (patellar height) between the groups (with and without medial patellofemoral ligament rupture).Conclusion; Patella elevation (patellar height) was similar in the group with and without medial patellofemoral rupture, while a significant difference was found in the trochlear groove angle.
Screening the best candidates for primary tumor resection among stage IV breast cancer patients, and identifying associated prognostic factors after surgery
Authors: Chi Changxing , Zhu Lujian , Zhao Yihan , Li Chenge , Zhu Yin , Chen Zhiyi , Zhao Youhong , Xu Yejin 
Published: February 2022

Abstract
Background
We analyzed overall survival (OS) following the surgical resection of primary tumor (PT) in patients with stage IV breast cancer (S4BC). We generated a predictive model to test our hypothesis that surgery will only benefit a subset of S4BC patients, depending on properties of PT, and patient responsiveness to other treatments, as well as PT resection.
Methods
For our analysis, we selected 5297 S4BC patients from the SEER database. We screened for potential prognostic indicators related to patients who received PT resection (PTR) surgery, using multivariate logistic regression analysis, in our attempt to identify an optimal patient population for PT surgery. Furthermore, we assessed the performance and practical value of our nomogram using calibration curves, receiver operating characteristic curves (ROC), and decision curve analysis (DCA) of our internal validation and external validation cohorts (VC).
Results
Overall, we selected 5297 eligible patients with S4BC. Among them, 2276 (42.97%) underwent PTR surgery. Based on our observation, the PTR surgery was an independent correlate of prolonged median OS duration in patients with S4BC, according to multivariate Cox analysis (49 months vs. 29 months, p:0.001). S4BC patients, who underwent PTR, were then arbitrarily separated into either a training (TC) or validation cohort (VC). Next, logistic regression analysis including age, gender, TN stage, histological type, tumor location, degree of differentiation, distant metastases occurrence, and treatment was used to construct a predictive model, which was then displayed as a clinical nomogram.
Conclusion
We established a nomogram that identified the optimal candidates for surgical PTR among patients with S4BC.
Multi-species meta-analysis identifies transcriptional signatures associated with cardiac endothelial responses in the ischaemic heart
Authors: Li Ziwen , Solomonidis Emmanouil , Berkeley Bronwyn , Tang Michelle , Stewart Katherine Ross , Perez-Vicencio Daniel , McCracken Ian , Spiroski Ana-Mishel , Gray Gillian , Barton Anna , Sellers Stephanie , Riley Paul , Baker Andrew , Brittan Mairi 
Published: February 2022

Abstract
Myocardial infarction remains the leading cause of heart failure. The adult human heart lacks the capacity to undergo endogenous regeneration. New blood vessel growth is integral to regenerative medicine necessitating a comprehensive understanding of the pathways that regulate vascular regeneration. We present a comprehensive meta-analysis of integrated single cell RNA-sequencing data of coronary vascular endothelial cells from the developing and adult mouse and human heart spanning healthy and acute and chronic ischaemic cardiac disease. We identify species-conserved gene regulatory pathways aligned to endogenous neovascularisation. We annotated injury-associated temporal shifts of the endothelial transcriptome and validated four genes: VEGF-C, KLF4, EGR1 and ZFP36. Moreover, we show that ZFP36 regulates human coronary endothelial cell proliferation and define that VEGF-C administration in vivo enhances clonal expansion of the cardiac vasculature post-myocardial infarction. Finally, we constructed a coronary endothelial cell meta-atlas, crescENDO, to empower future in-depth research to target pathways associated with coronary neovascularisation.