bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving the proteome coverage of Daphnia magna ‐ implications for future ecotoxicoproteomics studies
Authors: Wilde Magdalena V. , Brehm Julian , Schwarzer Michael , Stöckl Jan B. , Laforsch Christian , Fröhlich Thomas 
Published: February 2022

AbstractAquatic pollution is an increasing problem and requires extensive research efforts to understand associated consequences and to find suitable solutions. The crustacean Daphnia is a keystone species in lacustrine ecosystems by connecting primary producers with higher trophic levels. Therefore, Daphnia is perfectly suitable to investigate biological effects of freshwater pollution and is frequently used as an important model organism in ecotoxicology. The field of ecotoxicoproteomics has become increasingly prevalent, as proteins are important for an organism's physiology and respond rapidly to changing environmental conditions. However, one obstacle in proteome analysis of Daphnia is highly abundant proteins like vitellogenin, decreasing the analytical depth of proteome analysis. To improve proteome coverage in Daphnia, we established an easy‐to‐use procedure based on the LC‐MS/MS of whole daphnids and the dissected Daphnia gut, which is the main tissue getting in contact with soluble and particulate pollutants, separately. Using a comprehensive spectral library, generated by gas‐phase fractionation and a data‐independent acquisition method, we identified 4621 and 5233 protein groups at high confidence (false discovery rate < 0.01) in Daphnia and Daphnia gut samples, respectively. By combining both datasets, a proteome coverage of 6027 proteins was achieved, demonstrating the effectiveness of our approach.
Molecular evidences on anti‐inflammatory, anticancer, and memory‐boosting effects of frankincense
Authors: Khajehdehi Mina , Khalaj‐Kondori Mohammad , Baradaran Behzad 
Published: February 2022

AbstractChemical diversity of natural products with drug‐like features has attracted much attention from medicine to develop more safe and effective drugs. Their anti‐inflammatory, antitumor, analgesic, and other therapeutic properties are sometimes more successful than chemical drugs in controlling disease due to fewer drug resistance and side effects and being more tolerable in a long time. Frankincense, the oleo gum resin extracted from the Boswellia species, contains some of these chemicals. The anti‐inflammatory effect of its main ingredient, boswellic acid, has been traditionally used to treat many diseases, mainly those target memory functions. In this review, we have accumulated research evidence from the beneficial effect of Frankincense consumption in memory improvement and the prevention of inflammation and cancer. Besides, we have discussed the molecular pathways mediating the therapeutic effects of this natural supplement.
Berberine as a natural modulator of inflammatory signaling pathways in the immune system: Focus on NF‐κB, JAK/STAT, and MAPK signaling pathways
Authors: Haftcheshmeh Saeed Mohammadian , Abedi Maliheh , Mashayekhi Kazem , Mousavi Mohammad Javad , Navashenaq Jamshid Gholizadeh , Mohammadi Asadollah , Momtazi‐Borojeni Amir Abbas 
Published: February 2022

AbstractThree main inflammatory signaling pathways include nuclear factor‐κB (NF‐κB), Janus kinases/Signal transducer and activator of transcriptions (JAKs/STATs), and mitogen‐activated protein kinases (MAPKs) play crucial roles in inducing, promoting, and regulating inflammatory responses in the immune system. Importantly, the breakdown of mechanisms that tightly regulate inflammatory signaling pathways can be the underlying cause of uncontrolled inflammatory responses and be associated with the generation and development of several inflammatory diseases. Hence, therapeutic strategies targeting inflammatory signaling pathways and their downstream components may promise to treat inflammatory diseases. Studies over the past two decades have provided important information on the polytrophic pharmacological and biochemical properties of berberine (BBR) as a naturally occurring compound, such as antioxidant, antitumor, antimicrobial, and antiinflammatory activates. Interestingly, the modulatory effects of BBR on inflammatory signaling cascades, which lead to the inhibition of inflammation, have been widely investigated in several in vitro and in vivo studies. For the first time, herein, this comprehensive review attempts to put together these studies and provide important insight into the modulatory effects of BBR on NF‐κB, JAKs/STATs, and MAPKs signaling pathways in vitro in various types of immune cells and in vivo in several experimental inflammatory diseases. As the second achievement of this review, we also explore the therapeutic efficacy and antiinflammatory effects of BBR regarding its modulatory action.
Field assessment of horizontal accelerations using air ride suspension trailers
Authors: Dunno Kyle , Ge Changfeng 
Published: February 2022

AbstractCurrent laboratory tests designed to evaluate package and unit load systems focus strictly on the intensity of vertical orientation. However, due to an increased demand for source reduction and the light‐weighting of packaging components, unit loads are becoming more susceptible to the horizontal forces encountered during transport. Understanding the field measured acceleration levels is critical to developing accurate laboratory test simulations to examine unit load rigidity. This paper uses horizontal acceleration data collected from two air ride suspension trailers to quantify the rise times and steady‐state accelerations levels experienced by the vehicles in the longitudinal (braking) and lateral directions. These characteristics are essential in establishing test methods that accurately reflect field measured events. The results show that the rise times experienced by larger transport trailers are longer than previous reports using smaller trailers. The larger transport vehicles from the present study showed lower decelerations and accelerations in braking and lateral moving events, respectively, compared to smaller transport vehicles. Regardless of travel direction, the deceleration and acceleration levels are right‐skewed, with most of the events falling below 0.33 g.
Ordered Element Distributed C3N Quantum Dots Manipulated Crystallization Kinetics for 2D CsPbI3 Solar Cells with Ultra‐High Performance
Authors: Li Zhizai , Yang Siwei , Ye Caichao , Wang Gang , Ma Bo , Yao Huanhuan , Wang Qian , Peng Guoqiang , Wang Qiang , Zhang Hao‐Li , Jin Zhiwen 
Published: February 2022

AbstractTwo‐dimensional (2D) CsPbI3 is developed to conquer the phase‐stability problem of CsPbI3 by introducing bulky organic cations to produce a steric hindrance effect. However, organic cations also inevitably increase the formation energy and difficulty in crystallization kinetics regulation. Such poor crystallization process modulation of 2D CsPbI3 leads to disordered phase‐arrangement, which impedes the transport of photo‐generated carriers and worsens device performance. Herein, a type of C3N quantum dots (QDs) with ordered carbon and nitrogen atoms to manipulate the crystallization process of 2D CsPbI3 for improving the crystallization pathway, phase‐arrangement and morphology, is introduced. Combination analyses of theoretical simulation, morphology regulation and femtosecond transient absorption (fs‐TA) characterization, show that the C3N QDs induce the formation of electron‐rich regions to adsorb bulky organic cations and provide nucleation sites to realize a bi‐directional crystallization process. Meanwhile, the quality of 2D CsPbI3 film is improved with lower trap density, higher surface potential, and compact morphology. As a result, the power conversion efficiency (PCE) of the optimized device (n = 5) boosts to an ultra‐high value of 15.63% with strengthened environmental stability. Moreover, the simple C3N QDs insertion method shows good universality to other bulky organic cations of Ruddlesden‐Popper and Dion‐Jacobson, providing a good modulation strategy for other optoelectronic devices.
Atomic‐Scale Mechanism of Spontaneous Polarity Inversion in AlN on Nonpolar Sapphire Substrate Grown by MOCVD
Authors: Liu Zhiqiang , Liu Bingyao , Ren Fang , Yin Yue , Zhang Shuo , Liang Meng , Dou Zhipeng , Liu Zhetong , Yang Shenyuan , Yan Jianchang , Wei Tongbo , Yi Xiaoyan , Wu Chaoxing , Guo Tailiang , Wang Junxi , Zhang Yong , Li Jinmin , Gao Peng 
Published: February 2022

AbstractThe performance of nitride devices is strongly affected by their polarity. Understanding the polarity determination and evolution mechanism of polar wurtzite nitrides on nonpolar substrates is therefore critically important. This work confirms that the polarity of AlN on sapphire prepared by metal–organic chemical vapor deposition is not inherited from the nitrides/sapphire interface as widely accepted, instead, experiences a spontaneous polarity inversion during the growth. It is found that at the initial growth stage, the interface favors the nitrogen‐polarity, rather than the widely accepted metal‐polarity or randomly coexisting. However, the polarity subsequently converts into the metal‐polar situation, at first locally then expanding into the whole area, driven by the anisotropy of surface energies, which results in universally existing inherent inverse grain boundaries. Furthermore, vertical two‐dimensional electron accumulation originating from the lattice symmetry breaking at the inverse grain boundary is first revealed. This work identifies another cause of high‐density defects in nitride epilayers, besides lattice mismatch induced dislocations. These findings also offer new insights into atomic structure and determination mechanism of polarity in nitrides, providing clues for its manipulation toward the novel hetero‐polarity devices.
Confinement Assembly in Polymeric Micelles Enables Nanoparticle Superstructures with Tunable Molecular‐Like Geometries
Authors: Zhang Zhebin , Xia Yan , Wan Siyu , Yang Dong , Dong Angang 
Published: February 2022

AbstractThe self‐assembly of a small number of nanoparticles into superstructures that mimic the geometry of molecules provides an unprecedented route for creating materials with precisely defined structures and potentially programmable functionalities. Such nanoparticle clusters (NPCs), also known as colloidal molecules, have a wide range of applications due to the decisive ensemble effect. Here, a universal and straightforward strategy is developed to construct NPCs with tunable molecular‐like geometries by confining the self‐assembly of hydrophobic nanoparticles within micelles formed by amphiphilic copolymers. It is found that confinement assembly of both spherical and anisotropic nanoparticles can lead to NPCs, the molecular‐like conformation of which is widely tunable by adjusting the ratio between copolymers and nanoparticles. Mechanistic studies reveal the formation of large‐vesicle intermediates along the path toward forming NPCs. This work establishes a facile and general strategy of assembling finite nanoparticles with precisely tunable geometries without introducing any directional interactions, which can accelerate the exploration of clustered superstructures toward broad applications.
Does telemedicine save lives? Evidence on the effect of telemedicine parity laws on mortality rates
Authors: Chen Jiajia , Dills Angela K. 
Published: February 2022

AbstractBetween 1995 and 2018, just over half of U.S. states enacted laws requiring private insurance plans cover medical care provided remotely. These telemedicine parity laws likely increase health care access, particularly in areas with few providers, by granting patients access to specialists or primary care providers located elsewhere. We estimate the effect of telemedicine parity laws on mortality rates of all causes and for causes of death due to conditions more frequently treated with telemedicine. Mortality rates decline postparity laws, driven by decreases in ischemic heart disease deaths. Ischemic heart disease mortality rates decline by about 6% in the difference‐in‐differences specification and 9% in the event study estimation. These effects are concentrated in counties located in the fringes of metropolitan areas. We also estimate declines in hospital admissions postparity law, consistent with improved health outcomes. Our results suggest that relaxing current telemedicine regulations would reduce mortality rates.
Effect of Catalyst and Cross‐Linker Concentrations on the Functional and Chemical Properties of Sago Starch
Authors: Sondari Dewi , Restu Witta Kartika , Septevani Athanasia Amanda , Suryaningrum Riska , Burhani Dian , Widyaningrum Bernadeta Ayu , Putri Rahmawati 
Published: February 2022

AbstractThis study aims to analyze the chemical characteristics of sago starch cross‐linked with sodium trimetaphosphate (STMP) and sodium chloride as a catalyst. The modified sago is applied as a functional material, such as for a drug delivery matrix. The study investigates the effects of cross‐linker agents and catalysts (starch/STMP/catalyst) on swelling factor, solubility, and freeze‐thaw stability. The functional and chemical properties of commercial and modified sago starch are comparatively studied. Modified sago starch samples are produced by adding various amounts of sodium trimetaphosphate (0.5, 1.0, and 2.0 g) and the catalyst of sodium chloride (2.0 and 4.0 g) into the reactor in a water bath hotplate at 70 °C and 300 rpm for an hour. The spectra of Fourier transform infrared (FTIR) demonstrate new bands related to phosphorylation. The intensity of the vibration peak of –P = O bending at 1150–1414 cm–1 and P–O–C stretching at 995–1050 cm–1 appears in the spectra of the samples. The spectra from FTIR spectroscopy confirm that the commercial sago starch is successfully modified.
Extraction, Bioactive Composition, and Antioxidant Activity of Polysaccharides from the Mushroom Rugiboletus extremiorientalis
Authors: Guo Xiao‐Ying , Guo Hong‐Bo , Wu Shuang‐Yan , Yu Xiao‐Dan 
Published: February 2022

AbstractThe wild, edible mushroom Rugiboletus extremiorientalis has an excellent taste and also medicinal effects, and is very popular in China. In this study, hot water extraction is used to isolate polysaccharides from R. extremiorientalis fruiting bodies, and response surface methodology is used to optimize the extraction conditions in terms of temperature, liquid–solid ratio, and time. The crude extract (REP) is purified in turn by DEAE‐52 chromatography and Sephadex G‐100 chromatography, resulting in three fractions (REP‐1, REP‐2, and REP‐3) with recovery rates of 16.7%, 57.65%, and 6.63%, respectively. In vitro antioxidant activity is highest for REP‐2, and the subsequent research is therefore focused on that fraction. High‐performance liquid chromatography reveals that REP‐2 is comprised of fucose, arabinose, galactose, glucose, xylose, and mannose. Fourier‐transform infrared spectroscopy of REP‐2 indicates the presence of functional groups typical for polysaccharides. High‐performance gel filtration chromatography indicates the REP‐2 has a molecular weight of 342.4 kDa. The results of this study provide a foundation for future research concerning the antioxidant activity of polysaccharides in R. extremiorientalis.
The Work of Story Mediators
Authors: Cleland Silva Tricia , de Tarso Fonseca Silva Paulo 
Published: February 2022

AbstractIn this chapter, we describe how we work as Story Mediators to facilitate authenticstory exchange for making sense of social identities and embodied realities at work. Starting with our own personal and professional stories, we discuss how we enter the workplace with our own embodied lived experiences. In addition, we share how our defined values help us keep accountable to other storytellers and ourselves.
Introduction
Authors: Stępka Maciej 
Published: February 2022

AbstractThis chapter provides foundational information for the discussion conducted in further parts of the book. The Introduction includes a review of the existing literature and contemporary perspectives on migration and security, focusing on different modes and practices of securitisation. It outlines the aims and methods of the book, arguing for a more inclusive, interpretative and framing-centred reading of securitisation theory. In doing so, the chapter also clarifies the interpretative characteristics and scope of the research presented in the book. The introduction ends with an outline of the book, discussing its structure and the aims of each chapter.
Anomalies for anomalous symmetries
Authors: Karasik Avner 
Published: February 2022

Abstract
4d gauge theories with massless fermions typically have axial U(1) transformations that suffer from the ABJ anomaly. One can modify the theory of interest by adding more fields in a way that restores the axial symmetry, and use it to derive rigorous ’t Hooft anomaly matching conditions. These conditions are not valid for the original theory of interest, but for the modified theory. We show that the modification can be done in a specific way that allows us to relate the dynamics of the modified theory to the dynamics of the original theory. In this way, the anomaly matching conditions of the modified theory can be used to learn new things on the original theory even though they involve axial transformations which are not a symmetry of the original theory. We describe this method and discuss some applications to various examples.
Dark Matter interpretation of the neutron decay anomaly
Authors: Strumia Alessandro 
Published: February 2022

Abstract
We add to the Standard Model a new fermion χ with minimal baryon number 1/3. Neutron decay n → χχχ into non-relativistic χ can account for the neutron decay anomaly, compatibly with bounds from neutron stars. χ can be Dark Matter, and its cosmological abundance can be generated by freeze-in dominated at T ∼ mn. The associated processes n → χχχγ, hydrogen decay H → χχχν(γ) and DM-induced neutron disappearance $$ \overline{\chi} $$

χ
¯

n → χχ(γ) have rates below experimental bounds and can be of interest for future experiments.
Competitive Cooperation
Authors: Osganian Vanessa 
Published: February 2022

AbstractThis paper examines the institutional and social dimensions of cooperation in the Alliance of Science Organisations, the central corporatist stakeholder in German science policy, in the 1970s and 1980s, which were a crucial period for this committee. In doing so, this essay mainly focuses on the way science organizations interact with each other, as well as with national politics. The Federal Ministry of Research invited the Alliance to regular meetings and thereby fostered its involvement into political decision-making processes. Consequently, the question of who belonged to the Alliance came into the focus of different players. Although the members of the Alliance themselves decided on the composition of their committee, they were not able to completely insulate themselves from external demands. Including new members into the Alliance had a destabilizing effect on the carefully balanced distribution of power within this committee, as will be shown through the case study on the admission of the Association of the Major Research Centers (Arbeitsgemeinschaft der Großforschungseinrichtungen, AGF) in 1976. In order to restabilize the situation, the members of the Alliance tried to exclude the AGF from certain issues. At the same time, the AGF itself was keen on being regarded as an equal partner and thus strove for its inclusion. This complex interplay of cooperative and competitive actions finally resulted in the institutionalization of the Alliance.
Early DNA methylation changes in children developing beta cell autoimmunity at a young age
Authors: Starskaia Inna , Laajala Essi , Grönroos Toni , Härkönen Taina , Junttila Sini , Kattelus Roosa , Kallionpää Henna , Laiho Asta , Suni Veronika , Tillmann Vallo , Lund Riikka , Elo Laura L. , Lähdesmäki Harri , Knip Mikael , Kalim Ubaid Ullah , Lahesmaa Riitta 
Published: February 2022

Abstract
Aims/hypothesis
Type 1 diabetes is a chronic autoimmune disease of complex aetiology, including a potential role for epigenetic regulation. Previous epigenomic studies focused mainly on clinically diagnosed individuals. The aim of the study was to assess early DNA methylation changes associated with type 1 diabetes already before the diagnosis or even before the appearance of autoantibodies.

Methods
Reduced representation bisulphite sequencing (RRBS) was applied to study DNA methylation in purified CD4+ T cell, CD8+ T cell and CD4−CD8− cell fractions of 226 peripheral blood mononuclear cell samples longitudinally collected from seven type 1 diabetes-specific autoantibody-positive individuals and control individuals matched for age, sex, HLA risk and place of birth. We also explored correlations between DNA methylation and gene expression using RNA sequencing data from the same samples. Technical validation of RRBS results was performed using pyrosequencing.

Results
We identified 79, 56 and 45 differentially methylated regions in CD4+ T cells, CD8+ T cells and CD4−CD8− cell fractions, respectively, between type 1 diabetes-specific autoantibody-positive individuals and control participants. The analysis of pre-seroconversion samples identified DNA methylation signatures at the very early stage of disease, including differential methylation at the promoter of IRF5 in CD4+ T cells. Further, we validated RRBS results using pyrosequencing at the following CpG sites: chr19:18118304 in the promoter of ARRDC2; chr21:47307815 in the intron of PCBP3; and chr14:81128398 in the intergenic region near TRAF3 in CD4+ T cells.

Conclusions/interpretation
These preliminary results provide novel insights into cell type-specific differential epigenetic regulation of genes, which may contribute to type 1 diabetes pathogenesis at the very early stage of disease development. Should these findings be validated, they may serve as a potential signature useful for disease prediction and management.

Graphical abstract
Quality appraisal of clinical guidelines for recurrent urinary tract infections using AGREE II: a systematic review
Authors: Pat Jorik J. , Witte Lambertus P. W. , Steffens Martijn G. , Vernooij Robin W. M. , Marcelissen Tom A. T. , Fuentes Paulina , Garcia-Perdomo Herney A. , Pardo-Hernandez Hector , Blanker Marco H. 
Published: February 2022

Abstract
Introduction and hypothesis
Recommendations for preventing and diagnosing recurrent urinary tract infection (UTI) tend to vary between clinical practice guidelines (CPGs) because of low-quality scientific evidence, potentially leading to practice variation and suboptimal care. We assessed the quality of existing CPGs for recurrent UTI.

Methods
A systematic search was performed from January 2000 to June 2021 in PubMed and EMBASE for CPGs on recurrent UTI prevention or hospital diagnostics in Dutch, English, and Spanish. Each CPG was assessed by four appraisers in a multidisciplinary review team, using the Appraisal of Guidelines, Research, and Evaluation II (AGREE II) instrument.

Results
We identified and assessed eight CPGs published between 2013 and 2021. The scope and purpose (mean and standard deviation: 67.3 ± 21.8) and clarity of presentation (74.8 ± 17.6) domains scored highly. However, issues with methods, patient participation, conflict of interests, and facilitators and barriers were common and resulted in lower scores for the rigour of development (56.9 ± 25.9), applicability (19.6 ± 23.4), stakeholder involvement (50.4 ± 24.6), and editorial independence (62.1 ± 23.1) domains. Overall, two CPGs were recommended, three were recommended with modifications, and three were not recommended.

Conclusions
Significant room for improvement exists in the quality of CPGs for recurrent UTI, with most displaying serious limitations in the stakeholder involvement, rigour of development, and applicability domains. These aspects must be improved to decrease diagnostic and therapeutic uncertainty. Developers could benefit from using checklists and following guidelines when developing de novo CPGs.