bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Continuous Marine Sponge Cell Line Established
Authors: Hesp Kylie , Heijden Jans M.E. van der , Munroe Stephanie , Sipkema Detmer , Martens Dirk E. , Wijffels Rene H. , Pomponi Shirley A. 
Published: October 2022

Abstract
The potential of sponge-derived chemicals for pharmaceutical applications remains largely unexploited due to limited available biomass. Although many have attempted to culture marine sponge cells in vitro to create a scalable production platform for such biopharmaceuticals, these efforts have been mostly unsuccessful. We recently showed that Geodia barretti sponge cells could divide rapidly in M1 medium. In this study we established the first continuous marine sponge cell line, originating from G. barretti. G. barretti cells cultured in OpM1 medium, a modification of M1, grew more rapidly and to a higher density than in M1. Cells in OpM1 reached 1.74 population doublings after 30 minutes, more than 2-fold higher than the already rapid growth rate of 0.74 population doublings in 30 minutes in M1. The maximum number of population doublings increased from 5 doublings in M1 to at least 98 doublings in OpM1. Subcultured cells could be cryopreserved and used to inoculate new cultures. With these results, we have overcome a major obstacle that has blocked the path to producing biopharmaceuticals with sponge cells at industrial scale for decades.
An integrated modeling framework for groundwater contamination risk assessment in arid, data-scarce environments
Authors: Rafiei-Sardooi Elham , Ghazanfarpour Hossein , Azareh Ali , Parteli Eric J. R. , Faryabi Mohammad 
Published: October 2022

Abstract
Groundwater contamination risk mapping is one essential measure in groundwater management and quality control. The purpose of the present study is to address this mapping by means of a novel framework, which is more suitable for arid regions than other methods developed in previous work. Specifically, we integrate machine learning tools, interpolation and process-based models with a modified version of DRASTIC-AHP to evaluate groundwater vulnerability to nitrate contamination and to map this contamination in Jiroft plain, Iran. The DRASTIC model provides a tool for evaluating aquifer vulnerability by using seven parameters related to the hydrogeological setting (Depth to water, net Recharge, Aquifer media, Soil media, Topography, Impact of vadose zone, and hydraulic Conductivity), while the criteria ratings and weights of these parameters are evaluated by means of an Analytic Hierarchy Process (AHP). However, to obtain the risk map, the results about groundwater vulnerability are combined here with a contamination hazard map, which we estimate by applying ensemble modeling based, in part, on the occurrence probability predicted from Generalized Linear Model (GLM), Flexible Discriminant Analysis (FDA), and Support Vector Machine (SVM). Our integrated modeling framework provides an assessment of both regional patterns of groundwater contamination and an estimate of the impacts of the contamination based on socio-environmental variables, and is particularly suitable for applications based on limited amount of available data. The groundwater contamination risk map obtained from our case study shows that the central and southern regions of the Jiroft plain display high and very high contamination risk, which is associated with high production rate of urban waste in residential lands and an overuse of nitrogen fertilizers in agricultural lands. Therefore, our work is providing new modeling insights for the future assessment of groundwater contamination, with potential impacts for the management and control of water resources in arid and semi-arid environments.
Experiences of Patients with Cancer regarding Decentralization of Oncology Services at a Tertiary Hospital in the Eastern Cape
Authors: Jojo Lumkile Wilmot , Nkutu Nonyaniso Trustina 
Published: October 2022

Abstract
Background
The cancer burden is a global public health concern associated with high morbidities and mortalities. Low and middle-income countries are more affected including South Africa. Limited access to oncology services contributes to the late presentation, late diagnosis, and treatment of cancer. In the Eastern Cape, oncology services were previously centralized with negative effects on the quality of life of the already compromised health status of the oncology patients. To mitigate the situation, a new oncology unit was opened to decentralize oncology services in the province. Little is known about the experiences of consumers after this transformation. That prompted this inquiry.
Objective
This study aims to explore the experiences of cancer patients regarding the decentralization of oncology services to enhance the quality of life of these patients.
Methodology
: A qualitative approach with a descriptive, explorative, and contextual design was undertaken, to get the perspective of oncology recipients following the decentralization of oncology services at a selected public tertiary hospital in the Eastern Cape. After obtaining ethical clearance and permission to conduct the study, interviews were conducted with 19 participants. All interviews were transcribed verbatim against their audio recordings. Field notes were taken by the researcher. The concept of trustworthiness was used to ensure rigor throughout this study. Thematic analysis was done using Tesch’s approach to open coding in qualitative research.
Results
Seven themes emerged: 1) level of satisfaction; 2) waiting time; 3) human and material resources; 4) attitude of health care workers; 5) appropriate treatment and care, 6) access; and 7) improved infrastructural resources.
Conclusion
The majority of patients had positive experiences with the unit. The waiting time was acceptable, and medication was available. Access to services was improved. The staff had a positive attitude.
The Anti-Oxidant Effects of Coenzyme Q10 on Albino Rat Testicular Toxicity and Apoptosis Triggered by Bisphenol A
Authors: Eid Refaat A. , Abadi Alsaleem Mohammed , kott Attalla F. El , Zaki Mohamed Samir A. , Abd-Ella Eman M. 
Published: October 2022

Abstract
Polycarbonate plastics for packaging and epoxy resins are both made with the industrial chemical bisphenol A (BPA). This investigation looked at the histological structure, antioxidant enzymes, and albino rats' testis to determine how Coenzyme Q10 (CoQ10) impacts BPA toxicity. For the experiments, 18 adult male rats were broken into three groups: group 1 received no therapy, group 2 acquired BPA, and group 3 got daily BPA treatment accompanied by coenzyme Q10, one hour apart. All experiment done for 14 days. The biochemical biomarkers catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) were altered as a result of BPA exposure. The testicular histological architecture, which is made up of apoptosis, was also exaggerated. Furthermore, rats given BPA and CoQ10 treatment may experience a diminution in these negative BPA effects. These protective properties of CoQ10 may be correlated with the ability to eliminate oxidizing substances that can harm living species.The outcomes might support the hypothesis that CoQ10 prevented oxidative damage and boosted rats' stress responses when BPA was introduced. Thus, by shielding mammals from oxidative stress, CoQ10 aids in the growth and development of the animals.BPA is extremely hazardous to humans and can persist in tissues. Human reproductive functions are a worry due to human exposure to BPA, especially for occupational workers who are typically exposed to higher doses of BPA. As a result, in order to reduce the health risks, BPA usage must be minimized across a diverse range of industries, and improper plastic container handling must be prohibited. By giving CoQ10 to patients, BPA's harmful effects on reproductive structures and functions may be avoided.
Patient-reported outcomes measures of X-linked hypophosphataemia participants: findings from a prospective cohort study in the UK
Authors: Cole Sophie , Sanchez-Santos Maria T. , Kolovos Spyros , Javaid M. Kassim , Pinedo-Villanueva Rafael 
Published: October 2022

Abstract
Background
X-linked hypophosphataemia (XLH) is a rare genetic condition passed on through the X chromosome which causes multiple symptoms including weakened teeth, bones, and muscles. Due to the rarity of the condition, little is known about the health outcomes as reported by people with the disease. The objectives of this study were threefold: to characterise key patient reported outcome measures (PROMs) in adults with XLH, to identify clusters of symptom-severity groups based on PROMs, and to analyse the longitudinal progression of available PROMs.
Methods
Data from 48 participants from the Rare and Undiagnosed Diseases cohort Study (RUDY) was used to analyse both cross-sectional and longitudinal patient-reported outcomes. We analysed data for health-related quality of life (HRQL): EQ-5D-5L, SF-36 Physical Component Score (PCS), and SF-36 Mental Component Score (MCS), sleep: PSQI and ESS, fatigue: FSS and FACIT-F, pain: SF-MPQ-2 and PainDETECT, and mental well-being: HADS anxiety and depression. Summary statistics, tests of mean differences, mixed-effects models, and cluster analysis were used to describe and examine the various health dimensions of individuals with XLH.
Results
Overall mean scores were EQ-5D-5L=0.65, SF-36-PCS=32.7, and SF-36-MCS=48.4 for HRQL, ESS=5.9 and PSQI=8.9 for sleep, FSS=32.8 and FACIT-F=104.4 for fatigue, SF-MPQ-2=1.9, and 7% reporting neuropathic pain (PainDETECT) for pain, and HADS-depression=4.7 and HADS-anxiety =6.2 for mental well-being. 7% reported neuropathic pain (PainDETECT) for pain. Whilst many adults with XLH reported good outcomes, extreme or severe problems were reported across all outcomes. Cluster analysis identified that adults with XLH could be divided into two distinct groups, one reporting worse (35.3%) and the other better outcomes (64.7%) (less pain, fatigue, depression, and higher levels of sleep). Longitudinal analysis showed that FACIT-F and HADS-anxiety scores worsened slightly over two years with statistically significant (p<0.05) time coefficients (b=-2.135 and b=0.314, respectively).
Conclusion
Although about two thirds of adult participants of the RUDY cohort with XLH report good health outcomes, for a considerable third much worse outcomes are reported. More research is needed to examine why some experience good and others poor health outcomes and the characteristics which identify them.
An efficient point-of-interest recommendation for LOcation-based social networks system with spatio-temporal model
Authors: VUPPALA VENKATA PRAVEEN KUMAR , SAMBANDAM PAZHANIRAJAN , SREE INDRANEEL 
Published: October 2022

Abstract
The problem of personalized next point-of-interest (POI) recommendation is significant and of practical value in location-based social networks (LBSNs). Twitter together with other online social networks has begun to collect hundreds of millions of check-ins which capture the spatial and temporal information of user movements and interests. Due to the sparsity of data in regard to check-in, POI recommendations remain a challenging problem. An efficient point-of-interest recommendation system with Upstream Spatiotemporal Topic Model (USTTM) for LBSNs is proposed in this paper. This recommendation system contains purpose prediction phase that classifying the POIs in spatio-temporal database based on purpose and constructing a purpose ranking model to model the selection of user’s intended purpose for the trip. Second phase is scoring of each candidate POI which considers the properties of spatial and temporal information when calculating the score. USTTM is used in this system to model and analyze the spatio-temporal aspect of check-in data that can discover a user’s choice of region. Extensive experiments were conducted and the results demonstrate that the recommendation accuracy of the model outperforms the state-of-the-art POI recommendation models with good runtime performance. In quantitative analysis, effectiveness of USTTM in terms of accuracy of POI recommendation and accuracy of user and time prediction are evaluated and results show that the USTTM achieves better performance than the state-of-the-art models.
Variations in the type of nutritional support provided after pancreatoduodenectomy: results from an international multicentre retrospective cohort study
Authors: Russell Thomas , Labib Peter , Murphy Paula , Ausania Fabio , Pando Elizabeth , Roberts Keith , Kausar Ambareen , Mavroeidis Vasileios , Marangoni Gabriele , Thomasset Sarah , Frampton Adam , Lykoudis Pavlos , Maglione Mauel , Alhaboob Nassir , Bari Hassaan , Smith Andrew , Spalding Duncan , Sriniva Parthi , Davidson Brian , Bhogal Ricky , Croagh Daniel , Dominguez Ismael , Thakkar Rohan , Gomez Dhanny , Silva Michael , Lapolla Pierfrancesco , Mingoli Andrea , Porcu Alberto , Shah Nehal , Hammady Zaed , Al-Sarrieh Bilal , Serrablo Alejandro , Aroori Somaiah 
Published: October 2022

Abstract
Background/Objectives: An early oral diet is recommended after pancreatoduodenectomy (PD), however, the nutritional management of PD patients is known to be highly variable, and some centres still provide postoperative parenteral nutrition (PN) routinely. A proportion of patients who receive PN experience clinically significant complications underscoring its judicious use. Using a multicentre cohort, this study aimed to determine the proportion of PD patients that received postoperative nutritional support (NS), describe the variations in this support, and investigate whether receiving PN correlated with morbidity.
Subjects/Methods: Data was extracted from the Recurrence After Whipple’s (RAW) study database, a retrospective study of patients undergoing PD for histologically confirmed pancreatic, ampullary or distal bile duct malignancy.
Results: 1323 patients (89.2%) had data on their postoperative NS status available. Of these, 45.4% received postoperative NS. This was “enteral only”, “parenteral only”, and “enteral and parenteral” in 44.3%, 35.2% and 20.5% of cases, respectively. Body mass index <18.5 kg/m2 (p=0.03), absence of preoperative biliary stenting (p=0.009) and serum albumin <36 g/L (p=0.009) all correlated with receiving postoperative NS. Among those who did not develop a serious postoperative complication (Clavien-Dindo grade ≥IIIa), one-fifth received PN.
Conclusion: Being underweight, not undergoing preoperative biliary stenting and having a low preoperative serum albumin all correlated with receiving postoperative NS. A considerable number of patients who had an uneventful recovery received PN; this should be reserved for those who are unable to take an oral diet.
Selective disruption of perineuronal nets in mice lacking Crtl1 is sufficient to make fear memories susceptible to erasure
Authors: Poli Andrea , Viglione Aurelia , Mazziotti Raffaele , Morea Silvia , Melani Riccardo , Silingardi Davide , Putignano Elena , Berardi Nicoletta , Pizzorusso Tommaso 
Published: October 2022

Abstract
The ability to store, retrieve, and extinguish memories of adverse experiences is an essential skill for animals’ survival. The cellular and molecular factors that underlie such processes are only partially known. Using chondroitinase ABC treatment targeting chondroitin sulfate proteoglycans (CSPGs), previous studies showed that the maturation of the extracellular matrix makes fear memory resistant to deletion. Mice lacking the cartilage link protein Crtl1 (Crtl1-KO mice) display normal CSPG levels but impaired CSPG condensation in perineuronal nets (PNNs). Thus, we asked whether the presence of PNNs in the adult brain is responsible for the appearance of persistent fear memories by investigating fear extinction in Crtl1-KO mice. We found that mutant mice displayed fear memory erasure after an extinction protocol as revealed by analysis of freezing and pupil dynamics. Fear memory erasure did not depend on passive loss of retention; moreover, we demonstrated that, after extinction training, conditioned Crtl1-KO mice display no neural activation in the amygdala (Zif268 staining) in comparison to control animals. Taken together, our findings suggest that the aggregation of CSPGs into PNNs regulates the boundaries of the critical period for fear extinction.
Analysis of Cord Stress During Tire Dynamic Longitudinal Slip
Authors: Zhou Shuiting , Chen Luwen , Sun Pengfei , Meng Junling , Qian Chao , Sun Xin 
Published: October 2022

Abstract
Select 215/55 R17 radial tires for material test to obtain relevant material parameters. Use these parameters to build a finite element model and conduct longitudinal slip simulation. The relationship between longitudinal force and slip rate at different speeds and loads was determined by tire longitudinal slip tests. Then the longitudinal slip simulation results are compared with the test results to verify the feasibility of the model. The circumferential and axial Rebarforce of the belt layer at different speeds and the extreme value of the circumferential Rebarforce on the reverse-envelope of different skeleton materials are analyzed. The results show that: in the static state, the cord force of the belt layer will show a trend consistent with the direction of the cord arrangement. As the speed increases, the belt cord force exhibits significant fluctuations and asymmetry. The Rebarforce of the turn-up points on the belt and ply is significantly smaller compared to the fetal crown ply. The Rebarforce at the fetal crown and belt wrapping points at 60km/h is smaller than at 40km/h and 80km/h. The cord force of the fetal crown ply and the belt ply is much more affected by the load than that of the ply.
Smile and speech for screening Parkinson’s disease and cognitive decline: A crowdsourced cross-sectional study
Authors: Oyama Genko , Ogawa Mayuko , Morito Ken , Ishida Ryosuke , Urata Hiroshi , Lu Xiangxun , Kobayashi Tomohisa , Wooden Rina , Ota Susumu , Hatano Taku , Hattori Nobutaka 
Published: October 2022

Abstract
Smiling and speech are typically disturbed in patients with Parkinson’s disease (PD). This study aimed to find a subset of smile and speech features suitable for identifying PD and cognitive decline to build predictive models. This crowdsourced cross-sectional study collected smile data, conversational speech, and cognitive tests using a mobile chatbot app from 74 PD patients and 236 healthy controls (HC). In the classification model, the area under the curve for the accuracy of PD classification was 0.944 ± 0.029. In the regression model to predict the cognitive score of PD, linear regression showed a mean absolute error of 1.977 ± 0.309. A machine-learning model using a combination of smile and speech features obtained by a mobile chatbot app could differentiate between PD and HC. Furthermore, a model using a combination of smile and speech features might predict cognitive decline in PD.
Impact of legalization of same-sex marriage on the new HIV infection rate in 172 countries around the world based on difference-in-differences (DID) method
Authors: Jiang Kuan , Su Mintao , Zhang Zhongsong , Jia Jinzhu 
Published: October 2022

Abstract
Background: Acquired immunodeficiency syndrome is a major global health challenge, which is exceedingly harmful to human health. This study aimed to explore the impact of legalization of same-sex marriage on the new HIV infection rate in countries around the world.
Methods: The study is conducted on 172 countries whose observed data were collected from 1996 to 2019 in an open-source website. To evaluate the effect of legalization of same-sex marriage, difference of differences (DID) method was applied. The treatment effect was reported in static DID model, and the time-varied treatment effect and its 95% CI were reported in dynamic DID model.
Results: In the static DID model, we found that compared with countries without legalization of homosexuality, countries with legalization of same-sex marriage had an increase of 0.129‰ (95%CI: [-0.234,0.491]) in the new HIV infection rate, but the result was not statistically significant. In the dynamic DID model from 1996 to 2010, we found that the fifth year after the implementation of same-sex marriage legalization policy significantly increased the new HIV infection rate by 0.7‰. But in other years the effect are not significant after the policy enforcement. The implementation of same-sex marriage legalization policy has no significant effect on the new HIV infection rate during 1996-2019, but increase the rate in early year from 1996-2010, although the latter result seems incredible because of the unbalance of the sample in 1996-2010.
Conclusion: Based on current study, the policy on legalization of same-sex marriage has no significant effect on new HIV infection rate. It seems that legalizing same-sex marriage do not intervene HIV transmission, but more evidence is needed and the mechanism remains to be studied.
Cardiac Adipose Tissue Volume assessed by Computed Tomography is a specific and independent Predictor of Early Mortality and Critical Illness in COVID-19 in type 2-Diabetic Patients
Authors: Charpentier Etienne , Redheuil Alban , Bourron Olivier , Boussouar Samia , Lucidarme Olivier , Zarai Mohamed , Kachenoura Nadjia , Bouazizi Khaoula , Salem Joe-Elie , Hekimian Guillaume , Kerneis Mathieu , Amoura Zahir , Allenbach Yves , Hatem Stephane , Jeannin Anne-Caroline , Andreelli Fabrizio , Phan Franck 
Published: October 2022

Abstract
Background
Patients with type 2-diabetes mellitus (T2D), are characterized by visceral and ectopic adipose tissue expansion, leading to systemic chronic low-grade inflammation. As visceral adiposity is associated with severe COVID-19 irrespective of obesity, we aimed to evaluate and compare the predictive value for early intensive care or death of three fat depots (cardiac, visceral and subcutaneous) using computed tomography (CT) at admission for COVID-19 in consecutive patients with and without T2D.
Methods
Two hundred and two patients admitted for COVID-19 were retrospectively included between February and June 2020 and distributed in two groups: T2D or non-diabetic controls. Chest CT with cardiac (CATi), visceral (VATi) and subcutaneous adipose tissue (SATi) volume measurements were performed at admission. The primary endpoint was a composite outcome criteria including death or ICU admission at day 21 after admission. Threshold values of adipose tissue components predicting adverse outcome were determined.
Results
One hundred and eight controls (median age: 76[IQR:59–83], 61% male, median BMI: 24[22–27]) and ninety-four T2D patients (median age: 70[IQR:61–77], 70% male, median BMI: 27[24–31]), were enrolled in this study. At day 21 after admission, 42 patients (21%) had died from COVID-19, 48 (24%) required intensive care and 112 (55%) were admitted to a conventional care unit (CMU). In T2D, CATi was associated with early death or ICU independently from age, sex, BMI, dyslipidemia, CRP and coronary calcium (CAC). (p = 0.005). Concerning T2D patients, the cut-point for CATi was > 100 mL/m² with a sensitivity of 0.83 and a specificity of 0.50 (AUC = 0.67, p = 0.004) and an OR of 4.71 for early ICU admission or mortality (p = 0.002) in the fully adjusted model. Other adipose tissues SATi or VATi were not significantly associated with early adverse outcomes. In control patients, age and male sex (OR = 1.03, p = 0.04) were the only predictors of ICU or death.
Conclusions
Cardiac adipose tissue volume measured in CT at admission was independently predictive of early intensive care or death in T2D patients with COVID-19 but not in non-diabetics. Such automated CT measurement could be used in routine in diabetic patients presenting with moderate to severe COVID-19 illness to optimize individual management and prevent critical evolution.