bluuebunny/crossref_metadata_2025_split huggingface dataset:
Propofol enhances stem-like properties of glioma via GABAAR‐dependent Src modulation of ZDHHC5-EZH2 palmitoylation mechanism
Authors: Fan Xiaoqing , Gong Meiting , Yu Huihan , Yang Haoran , Wang Sheng , Wang Ruiting 
Published: August 2022

AbstractBackgroundPropofol is a commonly used anesthetic. However, its effects on glioma growth and recurrence remain largely unknown.MethodsThe effect of propofol on glioma growth was demonstrated by a series of in vitro and in vivo experiments (spheroidal formation assay, western blotting, and xenograft model). The acyl-biotin exchange method and liquid chromatography-mass spectrometry assays identified palmitoylation proteins mediated by the domain containing the Asp-His-His-Cys family. Western blotting, co-immunoprecipitation, quantitative real-time polymerase chain reaction, co-immunoprecipitation, chromatin immunoprecipitation, and luciferase reporter assays were used to explore the mechanisms of theγ-aminobutyric acid receptor (GABAAR)/Src/ZDHHC5/EZH2 signaling axis in the effects of propofol on glioma stem cells (GSCs).ResultsWe found that treatment with a standard dose of propofol promoted glioma growth in nude mice compared with control or low-dose propofol. Propofol-treated GSCs also led to larger tumor growth in nude mice than did vector-treated tumors. Mechanistically, propofol enhances the stem-like properties of gliomas through GABAAR to increase Src expression, thereby enhancing the palmitoylation of ZDHHC5-mediated EZH2 and Oct4 expression.ConclusionThese results demonstrate that propofol may promote glioma growth through the GABAAR-Src-ZDHHC5-EZH2 mechanism and are helpful in guiding the clinical use of propofol to obtain a better patient prognosis after the surgical resection of tumors.
Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages
Authors: Wen Yukang , Chen Zhengkun , Tian Yaqin , Yang Mei , Dong Qingshuang , Yang Yujiao , Ding Honglei 
Published: August 2022

AbstractAutophagy is an important conserved homeostatic process related to nutrient and energy deficiency and organelle damage in diverse eukaryotic cells and has been reported to play an important role in cellular responses to pathogens and bacterial replication. The respiratory bacterium Mycoplasma hyopneumoniae has been identified to enter porcine alveolar macrophages, which are considered important immune cells. However, little is known about the role of autophagy in the pathogenesis of M. hyopneumoniae infection of porcine alveolar macrophages. Our experiments demonstrated that M. hyopneumoniae infection enhanced the formation of autophagosomes in porcine alveolar macrophages but prevented the fusion of autophagosomes with lysosomes, thereby blocking autophagic flux and preventing the acidification and destruction of M. hyopneumoniae in low-pH surroundings. In addition, using different autophagy regulators to intervene in the autophagy process, we found that incomplete autophagy promoted the intracellular proliferation of M. hyopneumoniae. We also found that blocking the phosphorylation of JNK and Akt downregulated the autophagy induced by M. hyopneumoniae, but pathways related to two mitogen-activated protein kinases (Erk1/2 and p38) did not affect the process. Collectively, M. hyopneumoniae induced incomplete autophagy in porcine alveolar macrophages through the JNK and Akt signalling pathways; conversely, incomplete autophagy prevented M. hyopneumoniae from entering and degrading lysosomes to realize the proliferation of M. hyopneumoniae in porcine alveolar macrophages. These findings raise the possibility that targeting the autophagic pathway may be effective for the prevention or treatment of M. hyopneumoniae infection.
The incidence of TB and MDR-TB in pediatrics and therapeutic options: a systematic review
Authors: Harichander Sheetal , Wiafe Ebenezer , Mensah Kofi Boamah , Bangalee Varsha , Oosthuizen Frasia 
Published: August 2022

Abstract
Background
Tuberculosis (TB) is considered one of the top 10 causes of death worldwide and the leading cause of death from a single infectious agent. Multidrug-resistant (MDR) TB can affect people of all age groups, including children (aged 0–15 years). However, very little is known about the extent of this problem in children. This systematic review aims to investigate the incidence of TB and drug-resistant (DR) TB among the pediatric population. It also reviews the therapeutic options available to treat the condition.

Methods
A comprehensive search for all relevant evidence was conducted. The following databases were searched: MEDLINE, CINAHL, and Web of Science. The searched time frame was limited from January 1990 to December 2020 with a focus on the incidence of TB and MDR-TB among pediatrics and the therapeutic options available.

Results
A total of 537 articles were obtained via the selected databases. After title and abstract screening, 418 articles were excluded leaving 119 articles. Full-text screening was conducted on 119 articles, excluding a further 110 articles. Thus, 9 articles were subject to quality assessment and included in this review. The 9 articles represented the age group of 0–15 years and included both males and females. All studies included were of retrospective study design.

Discussion
The included studies mentioned a moderate increase in TB cases among pediatrics exacerbated by malnutrition, lack of bacille Calmette-Guérin (BCG) vaccination, and human immunodeficiency virus (HIV) coinfection. MDR-TB prevalence was especially high in South Africa. Drug therapy for both TB and MDR-TB yielded favorable outcomes among pediatrics. However, one of the biggest challenges with drug therapy includes the dosage forms available.

Systematic review registration
DOI: 10.17605/OSF.IO/G34NF
COVID-19 pandemic vaccination strategies of early 2021 based on behavioral differences between residents of Tokyo and Osaka, Japan
Authors: Yasuda Hidenori , Ito Fuyu , Hanaki Ken-ichi , Suzuki Kazuo 
Published: August 2022

Abstract
Background
During the fourth COVID-19 wave in Japan, marked differences became apparent in the scale of the epidemic between metropolitan Tokyo in eastern Japan and Osaka prefecture in western Japan.

Methods
Public epidemic data were analyzed, with performance of mathematical simulations using simplified SEIR models.

Results
The increase in the number of infected persons per 100,000 population during the fourth wave of expansion was greater in Osaka than in Tokyo. The basic reproduction number in Osaka was greater than in Tokyo. Particularly, the number of infected people in their 20 s increased during the fourth wave: The generation-specific reproduction number for people in their 20 s was higher than for people of other generations. Both Tokyo and Osaka were found to have strong correlation between the increase in the number of infected people and the average number of people using the main downtown stations at night. Simulations showed vaccination of people in their 60 s and older reduced the number of infected people among the high-risk elderly population in the fourth wave. However, age-specific vaccination of people in their 20 s reduced the number of infected people more than vaccination of people in their 60 s and older.

Conclusions
Differences in the epidemic between Tokyo and Osaka are explainable by different behaviors of the most socially active generation. When vaccine supplies are adequate, priority should be assigned to high-risk older adults, but if vaccine supplies are scarce, simulation results suggest consideration of vaccinating specific groups among whom the epidemic is spreading rapidly.
A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics
Authors: Wang Yanshuang , Li Xuemiao , Dance David A. B. , Xia Han , Chen Chen , Luo Nini , Li Anyang , Li Yanmei , Zhu Qiao , Sun Qinghui , Wu Xingyong , Zeng Yingfei , Chen Lin , Tian Shen , Xia Qianfeng 
Published: August 2022

Abstract
Background
Burkholderia pseudomallei is a tropical pathogen that causes melioidosis. Its intrinsic drug-resistance is a leading cause of treatment failure, and the few available antibiotics require prolonged use to be effective. This study aimed to assess the clinical potential of B. pseudomallei phages isolated from Hainan, China.

Methods
Burkholderia pseudomallei strain (HNBP001) was used as the isolation host, and phages were recovered from domestic environmental sources, which were submitted to the host range determination, lytic property assays, and stability tests. The best candidate was examined via the transmission electron microscope for classification. With its genome sequenced and analyzed, its protective efficacy against B. pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated, in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test.

Results
A phage able to lyse 24/25 clinical isolates was recovered. It was classified in the Podoviridae family and was found to be amenable to propagation. Under the optimal multiplicity of infection (MOI) of 0.1, an eclipse period of around 20 min and a high titer (1012 PFU/ml) produced within 1 h were demonstrated. This phage was found stabile at a wide range of temperatures (24, 37, 40, 50, and 60 °C) and pH values (3–12). After being designated as vB_BpP_HN01, it was fully sequenced, and the 71,398 bp linear genome, containing 93 open reading frames and a tRNA-Asn, displayed a low sequence similarity with known viruses. Additionally, protective effects of applications of vB_BpP_HN01 (MOI = 0.1 and MOI = 1) alone or in combination with antibiotics were found to improve viability of infected cells (70.6 ± 6.8%, 85.8 ± 5.7%, 91.9 ± 1.8%, and 96.8 ± 1.8%, respectively). A significantly reduced mortality (10%) and a decreased pathogen load were demonstrated in infected C. elegans following the addition of this phage.

Conclusions
As the first B. pseudomallei phage was isolated in Hainan, China, phage vB_BpP_HN01 was characterized by promising lytic property, stability, and efficiency of bacterial elimination during the in vitro/vivo experiments. Therefore, we can conclude that it is a potential alternative agent for combating melioidosis.

Graphical Abstract
Salt weathering impact on Nero/Ramses II Temple at El-Ashmonein archaeological site (Hermopolis Magna), Egypt
Authors: Fahmy Abdelrhman , Molina-Piernas Eduardo , Martínez-López Javier , Domínguez-Bella Salvador 
Published: August 2022

Abstract
Nero’s Temple at El-Ashmonein archaeological site, in Minia (middle of Egypt), is considered one of the most important of all temples of Nero. This temple dates back to 1520 BC–1075 BC in the New Kingdom and was reused in the era of Nero (the Fifth Emperor of Rome). The temple construction materials are severely decayed from surrounding environmental impacts, especially contaminated water sources. The main objective of this paper is to identify the construction materials of Nero’s Temple and their decay by-products. To achieve them, X-ray diffraction, micro X-ray fluorescence spectrometry, and portable Raman spectroscopy were utilized to identify the compositions and alteration by-products/degradation compounds (mainly saline efflorescence and crusts) from the construction materials, such as limestone walls, and structural mortars of the temple. In addition, a polarizing microscope was used to identify the minerals inside the construction materials and reveal the alteration of the minerals because of decay. Digital microscopy and scanning electron microscopy with EDS were used to detect decayed materials' morphological features. Finally, results showed that the main decay factor is salt attack (chlorides, sulfates, phosphates, nitrates, carbonates, and bicarbonates) for all architectural and structural elements of the temple, which have been carried to the construction materials from various sources of contaminated water (canal, sewage, and agricultural water).

Graphical Abstract
Feeding controls H2S production in situ in high solid anaerobic digestion
Authors: Ruxiang Cen , Ruiying Chen , Tianyun Pu , Chunyan Huang , Tengbing He , Guangliang Tian 
Published: August 2022

AbstractIn this study, a high frequency monitoring method was used to assess how semi-continuous feeding affects H2S production in high solid anaerobic digestion. The results showed that H2S characteristics at a monitoring frequency of 1 point/3 h were different to that of 1 point/24 h, its concentration decreased from 3449 ± 227 mg/m3 at 0 h to 298 ± 45 mg/m3 at 3 h. H2S concentration was negatively correlated with volatile fatty acids (VFAs), and oxidation reduction potential (ORP). 72–82% of H2S reduction in the first 3 h resulted from the introduction of O2 during feeding, and 18–28% of that was closely related to the production of a large quantity of soluble acidic matter, such as VFAs. A more accurate H2S release model was established according to the content of VFAs. Totally, this study implies that feed carrying air is a promising method for in situ control of H2S production in anaerobic digestion.
Graphical Abstract
Comparison and selection of patient follow-up systems for covid-19 pandemic patients
Authors: Eren Tamer , Danışan Tuğba , Deringöz Ayşegül , Aksüt Güler 
Published: August 2022

AbstractPeople have struggled with many infectious diseases throughout history. Today, the Covid-19 is being fought. One of the most important things for people who have or are at risk of getting Covid-19 is social isolation. Many countries resort to different ways to ensure social isolation. For this, remote patient monitoring systems have been developed. In this study, the problem of the selection of Covid-19 remote patient monitoring systems is discussed. Seven Wearable Health Technology (WHT) products were evaluated with a total of 10 criteria, including the important symptoms used in the patient tracking systems. The weights of 10 criteria determined by the Analytical Hierarchy Process (AHP) method were calculated, and these weights were used in the solution of The Preference Ranking Organization Method for Enrichment Evaluation (PROMETHEE), and Technique for Order Preference by Similarity to Ideal Solutions (TOPSIS) methods. WHT products were compared. As a result, the most appropriate patient follow-up system was determined. This study generates differences in terms of evaluating seven different products and ten criteria in total with MCDM methods. A more comprehensive evaluation has been made in the literature than the studies in this field.
Association of vitamin D deficiency and pelvic organ prolapse in postmenopausal women: a cross-sectional study
Authors: Legan Mateja , Barbič Matija , Osredkar Joško , Blaganje Mija 
Published: August 2022

Abstract
Background
Vitamin D is vital for skeletal integrity as well as optimal muscle work. High incidence and prevalence of vitamin D deficiency as well as pelvic organ prolapse are found in postmenopausal women, thus raising the question of whether the entities could be related.

Methods
We compared 50 postmenopausal women aged 50 to 75 years with pelvic organ prolapse (POP) with 48 women of same age without POP. The clinical assessment of the disorder was performed using the Pelvic Organ Prolapse Quantification system (POP-Q). An anamnestic questionnaire was filled out by the participants on their anthropometric data, life habits, reproductive history, previous and actual diseases. A blood sample was collected for determination of 25-OH-vitamin D as well as calcium and phosphorus concentrations.

Results
The group with POP and the control group were comparable in body mass index, physical activity, life habits and general health, but differed significantly in parity (being higher in POP) and vitamin D blood level concentrations, being lower in POP patients. A significantly higher prevalence of vitamin D deficiency (25-OH-vitamin D < 50 nmol/l) was found in the POP group compared to controls. Taking into account the confounding variables the logistic regression model confirmed the significant role of vitamin D for POP.

Conclusions
Vitamin D deficiency might be an important systemic factor associated to pelvic organ prolapse. The determination of vitamin D levels in postmenopausal women and replenishing its deficiency might also be of importance for the pelvic floor.
Entomopathogenic bacteria Photorhabdus luminescens as natural enemy against the African migratory locust, Locusta migratoria migratorioides (Reiche & Fairmaire, 1849) (Orthoptera: Acrididae)
Authors: Muhammad Jihan , Fathy Zeinab , Moussa Saad 
Published: August 2022

Abstract
Background
The African Migratory Locust, Locusta migratoria migratorioides (Reiche & Fairmaire, 1849) (Orthoptera: Acrididae), is a major threat to agricultural crops and food security on a worldwide scale; hence, maintaining control over it is crucial. Photorhabdus luminescens bacteria can accomplish the efficient biocontrol agent criteria. As a result, the aim of this study was to assess the efficacy of the P. luminescens (EGAP3) strain and its cell-free filtrate against L. migratoria migratorioides, as well as to investigate changes in the activity of carbohydrates hydrolyzing enzymes, amylase, invertase, and trehalase in whole-body homogenates of the 5th nymphal instar under laboratory conditions and to investigate the histopathological changes in the midgut of the locust.

Results
The virulence of entomopathogenic bacteria was determined at different densities of 4 × 107, 4 × 106, 4 × 105, and 4 × 104 colony-forming units (CFU)/ml at different exposure times as well as different concentrations of its cell-free filtrate, undiluted cell-free filtrate (100, 50, 25, and 12.5%). The results indicated that higher-density cell suspension up to 4 × 107 cells.ml−1 and undiluted cell-free filtrate (100%) were the most effective insecticidal fluids, reaching up to 76.7 and 80%, respectively, after 7 days. The estimated LC50 value was 2.7 × 106 cells.ml−1 for bacteria, and the estimated LC50 value for cell-free filtrate was the 2nd dilution (50%).

Conclusions
The data clarified the toxicological and histopathological effects and carbohydrate hydrolyzing enzyme activities of the host insect L. migratoria migratorioides, following bacteria and cell-free filtrate infection; that provides an overview of the efficiency of bacteria and their cell-free filtrate on the host. In conclusion, P. luminescens (EGAP3) and its toxins can be an optimal option for bio-controlling of L. migratoria migratorioides.
Treatment of ruptured rectal artery aneurysm in a patient with neurofibromatosis
Authors: Nemoto Hidehiko , Mori Kensaku , Takei Yohei , Kikuchi Shunsuke , Hoshiai Sodai , Yamamoto Yoshiyuki , Nakajima Takahito 
Published: August 2022

AbstractBackgroundSuperior rectal artery (SRA) aneurysms are rare. Although melena is the most common symptom, it has not been observed in cases of aneurysms located in the SRA trunk. Here, we report a case of a ruptured SRA trunk aneurysm successfully treated with coil embolization. Including our case, three of the four reported cases of SRA trunk aneurysms were related to neurofibromatosis type 1 (NF1).Case presentationA 52-year-old woman with NF1 was referred to our hospital for the investigation of an abdominal mass with back pain. She had previously undergone a blood transfusion at another hospital for anemia without melena. Computed tomography angiography revealed a ruptured SRA trunk aneurysm measuring 3 cm in diameter and surrounded by a retroperitoneal hematoma. The aneurysm was isolated by embolizing the SRA trunk distally and proximally. Distal embolization was performed retrogradely from the internal iliac artery (IIA) via the middle rectal artery (MRA)-SRA anastomosis because the antegrade approach from the inferior mesenteric artery (IMA) failed. To our knowledge, this is the first case of successful coil embolization of an IMA branch through the IIA.ConclusionSRA trunk aneurysms are rare; however, they are frequently associated with NF1. Antegrade distal embolization beyond the aneurysm is sometimes difficult to achieve. In such cases, a retrograde approach via MRA-SRA anastomosis can be the choice for isolating SRA trunk aneurysms.
Modified and improved performance of local hammermill for mushroom residues chopping
Authors: Amer Mariam A. , Ali El-Sayed A. E. , Dawood Victor M. 
Published: August 2022

Abstract
Background
During the mushroom production process, about one-fifth of the mushroom gets lost. The mushroom residues (MR) are rich in nutrients and can be utilized in diverse applications. Therefore, the goal of this research was to modify a local hammermill to improve the performance of chopping mushroom residues to be efficient used as a by-product.

Results
The experiments were conducted on a hammermill without and with six screen diameters, three drum rotational speeds, three feed rates, two average moisture content and two hammer rotation tracks (long–short). Then, chopper experiments were carried out with a focus on a specified size, power consumption and energy required. The findings of using any size screen offered little productivity since after a short time, the chopping MR was built blocks around the drum and blades. But using no screen gives these phenomena disappear. The chopping MR performed well in long track conditions, with a feeding rate of 700 kg/h, a drum speed of 300 rpm and moisture content of 43% .

Conclusions
The chopping operation had the best at feed rate of 700 kg/h, drum speed of 3000 rpm and 43% moisture content of which gave the appropriate MWD of 5.54 and 5.32 mm, consumed power of 1114.35 and 1189.125 W and required specific energy of 1.59 and 1.7 kW h/Mg for short and long tracks, respectively, and also largest mean weight diameter under such conditions due to a decrease in the amount of mushroom residues and an increase in their dryness, which increases the fragmentation impact of hammers on mushroom remnants.
Biomechanical validation of additively manufactured artificial femoral bones
Authors: Metzner F. , Neupetsch C. , Carabello A. , Pietsch M. , Wendler T. , Drossel W.-G. 
Published: August 2022

AbstractReplicating the mechanical behavior of human bones, especially cancellous bone tissue, is challenging. Typically, conventional bone models primarily consist of polyurethane foam surrounded by a solid shell. Although nearly isotropic foam components have mechanical properties similar to cancellous bone, they do not represent the anisotropy and inhomogeneity of bone architecture. To consider the architecture of bone, models were developed whose core was additively manufactured based on CT data. This core was subsequently coated with glass fiber composite. Specimens consisting of a gyroid-structure were fabricated using fused filament fabrication (FFF) techniques from different materials and various filler levels. Subsequent compression tests showed good accordance between the mechanical behavior of the printed specimens and human bone. The unidirectional fiberglass composite showed higher strength and stiffness than human cortical bone in 3-point bending tests, with comparable material behaviors being observed. During biomechanical investigation of the entire assembly, femoral prosthetic stems were inserted into both artificial and human bones under controlled conditions, while recording occurring forces and strains. All of the artificial prototypes, made of different materials, showed analogous behavior to human bone. In conclusion, it was shown that low-cost FFF technique can be used to generate valid bone models and selectively modify their properties by changing the infill.