bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel strategy for deliveringNiemann‐Pick typeC2proteins across the blood–brain barrier using the brain endothelial‐specificAAV‐BR1virus
Authors: Rasmussen Charlotte Laurfelt Munch , Hede Eva , Routhe Lisa Juul , Körbelin Jakob , Helgudottir Steinunn Sara , Thomsen Louiza Bohn , Schwaninger Markus , Burkhart Annette , Moos Torben 
Published: May 2022

AbstractTreating central nervous system (CNS) diseases is complicated by the incapability of numerous therapeutics to cross the blood–brain barrier (BBB), mainly composed of brain endothelial cells (BECs). Genetically modifying BECs into protein factories that supply the CNS with recombinant proteins is a promising approach to overcome this hindrance, especially in genetic diseases, like Niemann Pick disease type C2 (NPC2), where both CNS and peripheral cells are affected. Here, we investigated the potential of the BEC‐specific adeno‐associated viral vector (AAV‐BR1) encoding NPC2 for expression and secretion from primary BECs cultured in anin vitroBBB model with mixed glial cells, and in healthy BALB/c mice. Transduced primary BECs had significantly increased NPC2 gene expression and secreted NPC2 after viral transduction, which significantly reversed cholesterol deposition in NPC2 deficient fibroblasts. Mice receiving an intravenous injection with AAV‐BR1‐NCP2‐eGFP were sacrificed 8 weeks later and examined for its biodistribution and transgene expression of eGFP and NPC2. AAV‐BR1‐NPC2‐eGFP was distributed mainly to the brain and lightly to the heart and lung, but did not label other organs including the liver. eGFP expression was primarily found in BECs throughout the brain but occasionally also in neurons suggesting transport of the vector across the BBB, a phenomenon also confirmedin vitro. NPC2 gene expression was up‐regulated in the brain, and recombinant NPC2 protein expression was observed in both transduced brain capillaries and neurons. Our findings show that AAV‐BR1 transduction of BECs is possible and that it may denote a promising strategy for future treatment of NPC2.image
Pitfalls of self‐reported measures of self‐control: Surprising insights from extreme debtors
Authors: Jia Lile , Yuen Wei Lun , Ong Qiyan , Theseira Walter Edgar 
Published: May 2022

AbstractObjectiveWe took a rare opportunity to examine whether extreme debtors have inflated assessment of their self‐control capacity, potentially rendering self‐reported measures ineffective as prediction tools for debt risks.MethodThe self‐control profiles of extreme debtors (n = 1442), whose credit card debt amounted to more than 12 months of their income, were compared with samples of the general population (n = 505) and students from an elite university (n = 1011) on a self‐reported measure, a behavioral intention measure, and an executive function measure.ResultsExtreme debtors reported the highest self‐control on the self‐reported measure but scored the lowest on the behavioral intention measure and executive function. This pattern was robust across different subsections of demographics and diverse reasons of indebtedness.ConclusionOur findings suggest that self‐reported measures may be more susceptible to psychological distortions than behavioral measures, a pitfall that past research has overlooked. Furthermore, we reveal a double whammy faced by extreme debtors: they have a low capacity to regulate their behavior but hold an illusory perception of high capacity, which may expose them to further debt accumulation. The insights from our study underscore the utility of examining extreme segments of the population for advancing psychological knowledge.
Infant feeding type and maternal sleep during the postpartum period: a systematic review and meta‐analysis
Authors: Srimoragot Manassawee , Hershberger Patricia E. , Park Chang , Hernandez Teri L. , Izci Balserak Bilgay 
Published: May 2022

SummaryThe aim of this meta‐analysis was to examine the associations among infant feeding types, sleeping habits, and maternal sleep postpartum. Databases including Cumulative Index of Nursing and Allied‐Health Literature (CINAHL), PubMed, and Google Scholar were searched in addition to reference lists from selected articles and other key references. A critical review of relevant articles from the data sources was conducted with attention to the infant feeding types and maternal night‐time sleep. The methodological quality was assessed systematically. The pooled mean difference was calculated. Narrative summaries were also used. A total of 6,472 participants from seven studies were included in the meta‐analysis. A random‐effects model demonstrated a significantly higher maternal night‐time sleep in breastfeeding mothers than non‐breastfeeding mothers with a pooled standardized mean difference of 0.24 h (95% confidence interval 0.03–0.46, p = 0.026). Co‐sleeping with infants during the night also increased the sleeping hours in breastfeeding mothers. Homogeneity was observed with a Tau2 of 0.0308 and I2 of 44.3%. Funnel plots, Egger's and Begg's tests revealed no evidence of publication bias. This systematic review and meta‐analysis demonstrated that breastfeeding may be associated with a longer night‐time sleep postpartum and the synthesis of the literature suggested that co‐sleeping with the infant was associated with longer sleep duration in breastfeeding women. Further research into factors involving maternal decisions on infant feeding types and their effects on maternal sleep is needed to better understand the mothers' attitude toward infant feeding and their own sleep.
Closing the hepatitis C treatment gap: United States strategies to improve retention in care
Authors: Jones Austin T. , Briones Christopher , Tran Torrence , Moreno‐Walton Lisa , Kissinger Patricia J. 
Published: May 2022

AbstractThe hepatitis C virus (HCV) treatment landscape is shifting given the advent of direct‐acting antivirals and a global call to action by the World Health Organization. Eliminating HCV is now an issue of healthcare delivery. Treatment is limited by the complexity of the HCV care continuum, expensive therapy and competing health burdens experienced by an underserved HCV population. The objective of this literature review was to assess strategies to improve retention in HCV care, with particular focus on those implemented in the United States. We identified barriers in HCV care retention and propose solutions to increase HCV treatment delivery. The following recommendations are herein described: improving the cohesion of health services through localized care and integrated case management, expanding the supply of non‐specialist HCV treatment providers, leveraging patient navigators and care coordinators, improving adherence through directly observed therapy and reducing cost barriers through value‐based payment and pharmaceutical subscription models.
New insights into the etiology, risk factors, and pathogenesis of pancreatitis in dogs: Potential impacts on clinical practice
Authors: Cridge Harry , Lim Sue Yee , Algül Hana , Steiner Jörg M. 
Published: May 2022

AbstractWhile most cases of pancreatitis in dogs are thought to be idiopathic, potential risk factors are identified. In this article we provide a state‐of‐the‐art overview of suspected risk factors for pancreatitis in dogs, allowing for improved awareness and detection of potential dog‐specific risk factors, which might guide the development of disease prevention strategies. Additionally, we review important advances in our understanding of the pathophysiology of pancreatitis and potential areas for therapeutic manipulation based thereof. The outcome of pathophysiologic mechanisms and the development of clinical disease is dependent on the balance between stressors and protective mechanisms, which can be evaluated using the critical threshold theory.
Cytomegalovirus viral interleukin‐10 in patients with Aspergillus infection and effects on clinical outcome
Authors: Huang Shiang‐Fen , Huang Yu‐Chi , Lee Chen‐Te , Chou Kun‐Ta , Chen Hsin‐Pai , Huang Chia‐Chang , Ji Dar‐Der , Chan Yu‐Jiun , Yang Ying‐Ying 
Published: May 2022

AbstractBackgroundHuman cytomegalovirus (CMV) is associated with aspergillosis, but the simultaneous presence of CMV viral interleukin‐10 (cmvIL‐10) and aspergillosis has never been investigated. CmvIL‐10 is produced by CMV‐infected cells and acts as an immune modulator during CMV infection. The aim of this study was to evaluate cmvIL‐10 levels in peripheral blood and its influence on the clinical outcomes of Aspergillus infection.MethodsPatients who visited or were admitted to the hospital with suspected Aspergillus infection, including invasive aspergillosis (IA) and chronic pulmonary aspergillosis (CPA), were prospectively enrolled. The cmvIL‐10, human IL‐10 (hIL‐10), IL‐1B, IL‐6, IL‐8, IFN‐γ, and TNF‐α levels in peripheral blood were measured.ResultsPatients with Aspergillus infection had a higher level of cmvIL‐10 than the control group (158 ± 305 vs 27.9 ± 30.4 pg/ml, p < .05). The level of cmvIL‐10 was not correlated with CMV viremia or end‐organ disease. The cmvIL‐10 but not hIL‐10 level was positively correlated with the IFN‐γ level (p < .05) and marginally negatively correlated with IL‐1B and IL‐8 levels (p < .1). In patients with CPA, a high level of cmvIL‐10 (≥100 pg/ml) was a poor prognostic factor for long‐term survival (p < .05). In contrast, CMV viremia or end‐organ disease was associated with poor survival in patients with IA (p = .05).ConclusionsAspergillus infection was associated with CMV coinfection with cmvIL‐10 in blood. A cmvIL‐10 concentration ≥100 pg/ml was a predictor for unfavourable outcome in CPA patients.
Changes and differences in school food standards (2010–2021) and free school meal provision during COVID‐19 across the UK: Potential implications for children's diets
Authors: McIntyre Rebecca Louise , Adamson Ashley J , Nelson Michael , Woodside Jayne , Beattie Shirley , Spence Suzanne 
Published: May 2022

AbstractThis paper explores changes to school food standards from 2010, free school meal provision during the COVID‐19 pandemic across the UK and potential implications for children's diets. To obtain information on UK school food policies and free school meal provision methods we reviewed several sources including news articles, policy documents and journal articles. School food is an important part of the UK's health agenda and commitment to improving children's diets. Each UK nation has food‐based standards implemented, however, only Scotland and Wales also have nutrient‐based standards. School food standards in each nation have been updated in the last decade. Universal free school meals are available for children in the first 3 years of primary school in England and the first 5 years of primary school in Scotland, with plans announced for implementation of free school meals for all primary schoolchildren in Scotland and Wales. There is a lack of consistent monitoring of school food across the UK nations, and a lack of reporting compliance to the standards. Each nation differed in its response and management of free school meals during COVID‐related school closures. Further, there are issues surrounding the monitoring of the methods to provide free school meal support during school closures. The role of school food has been highlighted during COVID‐19, and with this, there have been calls for a review of free school meal eligibility criteria. The need for improved and consistent monitoring of school food across the UK remains, as does the need to evaluate the impact of school food on children's diets.
Rampant nuclear–mitochondrial–plastid phylogenomic discordance in globally distributed calcifying microalgae
Authors: Kao Tzu‐Tong , Wang Tzu‐Haw , Ku Chuan 
Published: May 2022

Summary

Incongruent phylogenies have been widely observed between nuclear and plastid or mitochondrial genomes in terrestrial plants and animals. However, few studies have examined these patterns in microalgae or the discordance between the two organelles.

Here we investigated the nuclear–mitochondrial–plastid phylogenomic incongruence in Emiliania–Gephyrocapsa, a group of cosmopolitan calcifying phytoplankton with enormous populations and recent speciations. We assembled mitochondrial and plastid genomes of 27 strains from across global oceans and temperature regimes, and analyzed the phylogenomic histories of the three compartments using concatenation and coalescence methods.

Six major clades with varying morphology and distribution are well recognized in the nuclear phylogeny, but such relationships are absent in the mitochondrial and plastid phylogenies, which also differ substantially from each other. The rampant phylogenomic discordance is due to a combination of organellar capture (introgression), organellar genome recombination, and incomplete lineage sorting of ancient polymorphic organellar genomes. Hybridization can lead to replacements of whole organellar genomes without introgression of nuclear genes and the two organelles are not inherited as a single cytoplasmic unit.

This study illustrates the convoluted evolution and inheritance of organellar genomes in isogamous haplodiplontic microalgae and provides a window into the phylogenomic complexity of marine unicellular eukaryotes.
A replication of “an experimental test of the expectancy‐disconfirmation theory of citizen satisfaction”
Authors: Chen Wenna , Dong Binzizi , Hsieh Chih‐Wei , Lee M. Jin , Liu Ning , Walker Richard M. , Wang Yao , Wen Bo , Wen Wen , Wu Peiyi , Wu Xia , Zhang Jiasheng 
Published: May 2022

AbstractTo better understand citizen satisfaction with public services, public administration research has adopted the expectancy‐disconfirmation model in recent years. This model proposes that satisfaction is a function of perceived performance and expectations. Recent quantitative and experimental studies of the expectancy‐disconfirmation model have supported the framework. However, few replications have been conducted and none outside western contexts. We conducted two narrow, robust experimental replications of Van Ryzin (2013, Journal of Policy Analysis and Management, 32(3), pp. 597–614) in the Chinese cities of Hong Kong (in 2017) and Shenzhen (in 2021). We found support for the findings reported in Van Ryzin (2013) and concluded that the expectancy‐disconfirmation model holds promise in a variety of settings as a framework for measuring citizen satisfaction with public services.
Bearing Witness: The Duty of Non‐indifference and the Case for Reading the News
Authors: Brown Brookes 
Published: May 2022

AbstractIgnorance of current events is ordinarily treated as a moral failing. In this article, I argue that much of this ire is misplaced. The disengaged are no less positioned to do good or dispense beneficence, no more arrogant or complicit than those glued to the headlines. Nonetheless, I contend that citizens do have moral reason to remain informed – they ought not be indifferent to others. This, I show, provides a standing reason to pay attention to distant strangers: by bearing witness, we avoid indifference. It follows that we should reconsider our media diet and our critiques of the disengaged.
Genome‐wide association study revealed TaHXK3‐2A as a candidate gene controlling stomatal index in wheat seedlings
Authors: Li Shumin , Yu Shizhou , Zhang Yifang , Zhu Dehe , Li Fangfang , Chen Bin , Mei Fangming , Du Linying , Ding Li , Chen Lei , Song Jiancheng , Kang Zhensheng , Mao Hude 
Published: May 2022

AbstractStomata are important channels for the control of gas exchange between plants and the atmosphere. To examine the genetic architecture of wheat stomatal index, we performed a genome‐wide association study (GWAS) using a panel of 539 wheat accessions and 450 678 polymorphic single nucleotide polymorphisms (SNPs) that were detected using wheat‐specific 660K SNP array. A total of 130 SNPs were detected to be significantly associated with stomatal index in both leaf surfaces of wheat seedlings. These significant SNPs were distributed across 16 chromosomes and involved 2625 candidate genes which participate in stress response, metabolism and cell/organ development. Subsequent bulk segregant analysis (BSA), combined with GWAS identified one major haplotype on chromosome 2A, that is responsible for stomatal index on the abaxial leaf surface. Candidate gene association analysis revealed that genetic variation in the promoter region of the hexokinase gene TaHXK3‐2A was significantly associated with the stomatal index. Moreover, transgenic analysis confirmed that TaHXK3‐2A overexpression in wheat decreased the size of leaf pavement cells but increased stomatal density through the glucose metabolic pathway, resulting in drought sensitivity among TaHXK3‐2A transgenic lines due to an increased transpiration rate. Taken together, these results provide valuable insights into the genetic control of the stomatal index in wheat seedlings.
Taxonomic and thematic semantic relationships in picture naming as revealed by Laplacian‐transformed event‐related potentials
Authors: Anderson Elizabeth J. , Midgley Katherine J. , Holcomb Phillip J. , Riès Stephanie K. 
Published: May 2022

AbstractSemantically related concepts co‐activate when we speak. Prior research reported both behavioral interference and facilitation due to co‐activation during picture naming. Different word relationships may account for some of this discrepancy. Taxonomically related words (e.g., WOLF‐DOG) have been associated with semantic interference; thematically related words (e.g., BONE‐DOG) have been associated with facilitation. Although these different semantic relationships have been associated with opposite behavioral outcomes, electrophysiological studies have found inconsistent effects on event‐related potentials. We conducted a picture‐word interference electroencephalography experiment to examine word retrieval dynamics in these different semantic relationships. Importantly, we used traditional monopolar analysis as well as Laplacian transformation allowing us to examine spatially deblurred event‐related components. Both analyses revealed greater negativity (150–250 ms) for unrelated than related taxonomic pairs, though more restricted in space for thematic pairs. Critically, Laplacian analyses revealed a larger negative‐going component in the 300 to 500 ms time window in taxonomically related versus unrelated pairs which were restricted to a left frontal recording site. In parallel, an opposite effect was found in the same time window but localized to a left parietal site. Finding these opposite effects in the same time window was feasible thanks to the use of the Laplacian transformation and suggests that frontal control processes are concurrently engaged with cascading effects of the spread of activation through semantically related representations.
Arabidopsis B‐box transcription factors BBX20‐22 promote UVR8 photoreceptor‐mediated UV‐B responses
Authors: Podolec Roman , Wagnon Timothée B. , Leonardelli Manuela , Johansson Henrik , Ulm Roman 
Published: May 2022

SUMMARYPlants undergo photomorphogenic development in the presence of light. Photomorphogenesis is repressed by the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1), which binds to substrates through their valine–proline (VP) motifs. The UV RESISTANCE LOCUS 8 (UVR8) photoreceptor senses UV‐B and inhibits COP1 through the cooperative binding of its own VP motif and photosensing core to COP1, thereby preventing COP1 binding to substrates, including the basic leucine zipper (bZIP) transcriptional regulator ELONGATED HYPOCOTYL 5 (HY5). As a key promoter of visible light and UV‐B photomorphogenesis, HY5 requires coregulators for its function. The B‐box family transcription factors BBX20–BBX22 were recently described as HY5 rate‐limiting coactivators under red light, but their role in UVR8 signaling was unknown. Here we describe a hypermorphic bbx21‐3D mutant with enhanced photomorphogenesis, carrying a proline‐to‐leucine mutation at position 314 in the VP motif that impairs the interaction with and regulation by COP1. We show that BBX21 and BBX22 are UVR8‐dependently stabilized after UV‐B exposure, which is counteracted by a repressor induced by HY5/BBX activity. bbx20 bbx21 bbx22 mutants under UV‐B are impaired in hypocotyl growth inhibition, photoprotective pigment accumulation and the expression of several HY5‐dependent genes under continuous UV‐B, but the immediate induction of marker genes after exposure to UV‐B remains surprisingly rather unaffected. We conclude that BBX20–BBX22 contribute to HY5 activity in a subset of UV‐B responses, but that additional, presently unknown, coactivators for HY5 are functional in early UVR8 signaling.
Elliptical Trainer Redesign to Diversify Muscles Recruitment
Authors: Dweiri Yazan M. , Al-Dwairi Abdullah F. , Al-Zanina Mousa S. , Al Diabat Reham 
Published: May 2022

Abstract
This work aims to introduce simple-to-implement modifications to the elliptical trainer device to increase its utility with added new exercise options. The effectiveness of the introduced modifications was assessed on 51 subjects, with effectiveness representing the recruitment of a broader range of muscle groups with desired intensity levels. The improvements include a new in-phase mode, where bilateral body synchronization creates a skiing-like motion, and a variable range of motion through adjusting the stride length of a rotating-link mechanism. The impact of these modifications on muscle recruitment was assessed by recording surface electromyogram (sEMG) from eleven major muscles while performing a total of six exercise routines. The routines have various combinations of mode and intensity to cover the traditional mechanism and the newly introduced mechanism adjustments for comparative analysis. The results have shown that increasing the stride length increases the demand on lower limbs muscles during the antiphase mode while decreasing it on upper limb muscles. When comparing the two exercise modes, all muscle groups showed significantly higher activity in the in-phase mode except for thigh muscles (Hamstrings and Quadriceps). Hamstrings revealed significantly higher activity in the antiphase mode, while Quadriceps showed no significantly different activity between the two modes. The introduced design modifications are shown to diversify the demand on major skeletal muscles hence improving its functionality at low added cost. Furthermore, these results can be exploited to implement gradual physiotherapeutic rehabilitation plans targeting various muscle groups with desired intensity levels.
High-Speed Jet Injector for Pharmaceutical Applications
Authors: Hankare Priyanka , Agrawala Ashish , Menezes Viren 
Published: May 2022

Abstract
A shock wave-driven needle-free syringe was developed and tested for liquid jet delivery into an artificial skin model and porcine skin samples. The device could deliver an adequate volume of liquid to a depth sufficient for drug dissemination in skin samples. The device is equipped with a splash-proof conduit and a silencer for smooth operation. The concept is expected to minimize the pain of liquid injection by (a) minimally breaching the blood vessels in the skin, (b) reducing trauma and inflammation, and aiding in regeneration of the incised spot by the liquid of the jet, and (c) preserving most of the microcirculation system in the target, enabling an effective drug uptake. A theoretical model that predicts jet penetration into viscoelastic targets is derived and presented. A sound agreement has been observed between the experimental jet penetration depths and the corresponding theoretical predictions. The development can offer a cost-effective, minimally invasive health care solution for immunization and drug delivery.