bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machining Accuracy Enhancement of Five-axis Machine Tools by a New Sensitivity Analysis Method Based on Machining Error Model
Authors: Tao Haohao , Li Tongjie , Chen Feng , Fan Jinwei , Pan Ri , Yu Zhen 
Published: January 2022

Abstract
Identification of key geometric errors is an essential prerequisite for improving the machining accuracy of five-axis machine tools. This paper presents a new sensitivity analysis (SA) method to extract key geometric errors, and then to improve the machining performance of machine tools by compensating key geometric error components. Development of geometric error prediction model is involved to obtain geometric error values at arbitrary positions at first. Based on the multi-body system theory and flank milling theory, the machining error prediction model is developed, which considers 37 geometric errors. Then, a new SA method is introduced by taking the machining error model as sensitivity analysis model and taking the geometric errors as analytical factors. Meanwhile, a sensitivity index, which has the characteristics of simple expression and clear physical meaning, is proposed, i.e., the peak value of the machining error caused by each geometric error. Moreover, the simulations analysis is carried out to obtain the sensitivity coefficient of each geometric error and the key error components. Finally, the validity and correctness of the proposed method is demonstrated by the experiments. Furthermore, the SA method can be extended to multi-axis machine tools.
Trabecular Bone Score: a New Simple Tool for Evaluating Osteoporosis in Patients With Axial Spondyloarthritis
Authors: Saisirivechakun Pannarat , Mahakkanukrauh Ajanee , Pongchaiyakul Chatlert , Boonya-ussadorn Trirat , Narongroeknawin Pongthorn , Pakchotanon Rattapol , Assavatanabodee Paijit , Chaiamnuay Sumapa 
Published: January 2022

Abstract
Background: Axial spondyloarthritis (ax-SpA) increases the risk of osteoporosis and vertebral fractures (VFs). Although bone mineral density (BMD) is used to diagnose osteoporosis, it has some limitations in patients with ax-SpA. Trabecular bone score (TBS), a new tool that indirectly assesses bone microarchitecture, can be used to assess fracture risk; however, few studies have been conducted to investigate TBS in ax-SpA patients.Objectives: To compare TBS between ax-SpA patients and 1:1 sex- and age-matched healthy controls and determine factors associated with low TBS in ax-SpA patients. Methods: A cross-sectional study was conducted in two tertiary-care hospitals. A total of 137 ax-SpA patients and controls were enrolled. Demographics, disease characteristics, and osteoporosis-related factors were recorded. TBS and BMD at the lumbar spine and hip were measured by dual X-ray absorptiometry (DXA). Low TBS define as a TBS value <1.230. Vertebral fractures were evaluated by densitometric vertebral fracture assessment. Factors associated with low TBS were examined by logistic regression.Results: Most ax-SpA patients were male (75.9%) and tested positive for HLA-B27 (88.3%). The mean (SD) age was 42.8 (12.0) years. The mean (SD) of TBS in the ax-SpA patients was lower than those in the controls [1.402 (0.107) vs 1.440 (0.086), respectively; p=0.002]. The mean (SD) of lumbar BMD in the ax-SpA patients was higher than those in controls [1.186 (0.212) vs 1.087 (0.124), p<0.001], whereas the mean (SD) of femoral neck BMD in the ax-SpA group was lower than that in the controls [0.867 (0.136) vs 0.904 (0.155), p=0.038]. High disease severity as indicated by sacroiliac joint fusion and a high ASDAS score were associated with low TBS with the odds ratios (95% confidence interval) of 11.8 (1.2-115.4) and 5.2 (1.6-16.9), respectively. Conclusion: Ax-SpA patients had a higher prevalence of low TBS than controls. Sacroiliac joint fusion and a high ASDAS score were associated with low TBS.
Epigenetic Silencing of PTEN and Epi-transcriptional Silencing of MDM2 Underlied Resistance to Hypomethylating Agents in Myelodysplastic Syndrome
Authors: Lee Paul , Yim Rita Lok Hay , Miu Ka-Kai , Sin-Hang Fung , Liao Jinyue , Wang Zhangting , Li Jun , Yung Yammy , Chu Hiu-Tung , Yip Pui-Kwan , Lee Emily , Tse Eric , Kwong Yok-Lam , Gill Harinder 
Published: January 2022

Abstract
In myelodysplastic syndrome (MDS), resistance to hypomethylating agents (HMA) portends a poor prognosis, underscoring the importance of understanding the molecular mechanisms leading to HMA-resistance. In this study, P39 and Kasumi-1 cells and their azacitidine-resistant and decitabine-resistant sublines were evaluated comparatively with transcriptomic and methylomic analyses. Expression profiling and genome-wide methylation microarray showed downregulation of PTEN associated with DNA hypermethylation in P39 cell lines resistant to azacitidine and decitabine. This pattern of PTEN dysregulation was also confirmed in a cohort of patients failing treatment with HMA. DNA hypomethylation of MDM2 was detected with downregulation of MDM2 in HMA resistant cell lines. Long-read sequencing revealed significant RNA hypomethylation of MDM2 resulting in alternative splicing and production of a truncated MDM2 transcript in azacitidine-resistant P39 cells. The expression of this MDM2 truncated transcript was also significantly increased in HMA-resistant patients compared with HMA-responsive patients. In conclusion, epigenetic and epi-transcriptomic dysregulation of PTEN and MDM2 were associated with resistance to hypomethylating agents.
Talaromyces Marneffei Mp1p Antigen Detection May Play an Important Role in The Early Diagnosis of Talaromycosis in Patients with Acquired Immunodeficiency Syndrome
Authors: Chen Xiaoman , Ou Xu , Wang Haodi , Li Liya , Guo Pengle , Chen Xiejie , Cai Weiping , Tang Xiaoping , Li Linghua 
Published: January 2022

Abstract
Talaromycosis is a life-threatening fungal disease commonly seen in patients with acquired immunodeficiency syndrome (AIDS), which is endemic in Southern China and Southeast countries. The diagnostic methods available for talaromycosis are relatively time-consuming and yield a high mortality. Therefore, early diagnosis of talaromycosis is extremely important. We aimed to determine a potential method for assisting in its early diagnosis. A total of 283 patients with AIDS admitted to our hospital were prospectively included in this cross-sectional study and divided into those with Talaromyces marneffei (TSM group, n=93) and those without Talaromyces marneffei (non-TSM group, n=190). The diagnostic accuracy of the Mp1p enzyme immunoassay (EIA), galactomannan (GM) assay, and blood culture performed within 3 days of hospitalisation were evaluated, using talaromycosis confirmed by culture and/or pathology as the gold standard. The positivity rates in the Mp1p EIA, GM assay, and blood culture were 72%, 64.5%, and 81.7%, respectively, in the TSM group. The sensitivity, specificity, and positive and negative predictive values of the Mp1p EIA were 72.0% (67/93), 96.8% (184/190), 91.8% (67/73), and 87.6% (184/210), respectively. The Mp1p EIA showed a substantial agreement with the gold standard (kappa: 0.729) and superiority to the GM assay (kappa: 0.603); it also showed a superior diagnostic accuracy in the patients with CD4+ counts of <50 cells/µL compared to those with CD4+ counts ranged from 50–100 cells/µL. The Mp1p EIA has the advantage of assisting in the early diagnosis of talaromycosis in patients with AIDS, especially those with low CD4+ counts.
The 3-Oxoacyl-(Acyl-Carrier-Protein) Reductase HSD-X1 of Pseudomonas
Citronellolis SJTE-3 Catalyzes the Conversion of 17β-estradiol to Estrone
Authors: Liang Rubing , Fu Yali , Peng Wanli , Lin Shuangjun , Deng Zixin 
Published: January 2022

Background:
Pseudomonas citronellolis SJTE-3 can efficiently degrade 17β-estradiol
(E2) and other estrogenic chemicals. However, the enzyme responsible for E2 metabolism within
strain SJTE-3 has remained unidentified.

Objective:
Here, a novel 3-oxoacyl-(acyl-carrier protein) (ACP) reductase, HSD-X1 (WP_
009617962.1), was identified in SJTE-3 and its enzymatic characteristics for the transformation
of E2 were investigated.

Methods:
Multiple sequence alignment and homology modelling were used to predict the protein
structure of HSD-X1. The concentrations of different steroids in the culture of recombinant strains
expressing HSD-X1 were determined by high performance liquid chromatography. Additionally,
the transcription of hsd-x1 gene was investigated using reverse transcription and quantitative PCR
analysis. Heterologous expression and affinity purification were used to obtain recombinant HSD-
X1.

Results:
The transcription of hsd-x1 gene in P. citronellolis SJTE-3 was induced by E2. Multiple
sequence alignment (MSA) indicated that HSD-X1 contained the two consensus regions and
conserved residues of short-chain dehydrogenase/reductases (SDRs) and 17β-hydroxysteroid dehydrogenases
(17β-HSDs). Over-expression of hsd-x1 gene allowed the recombinant strain to degrade
E2. Recombinant HSD-X1 was purified with a yield of 22.15 mg/L and used NAD+ as its cofactor
to catalyze the oxidization of E2 into estrone (E1) while exhibiting a Km value of 0.025 ±
0.044 mM and a Vmax value of 4.92 ± 0.31 mM/min/mg. HSD-X1 could tolerate a wide range of
temperature and pH, while the presence of divalent ions exerted little influence on its activity. Further,
the transformation efficiency of E2 into E1 was over 98.03% across 15 min.

Conclusion:
Protein HSD-X1 efficiently catalyzed the oxidization of E2 and participated in estrogen
degradation by P. citronellolis SJTE-3.
Expression Profiles of Long Noncoding and Messenger RNAs in Epicardial
Adipose Tissue-Derived from Patients with Coronary Atherosclerosis
Authors: Du Yu , Zhu Yong , Liu Yan , Liu Jinxing , Hu Chengping , Sun Yan , Zhang Dai , Lv Sai , Cheng Yujing , Han Hongya , Zhang Jianwei , Zhao Yingxin , Zhou Yujie 
Published: January 2022

Background:
Given its close anatomical location to the heart and its endocrine properties,
attention on epicardial adipose tissue (EAT) has increased.

Objective:
This study investigated the expression profiles of long noncoding RNAs (lncRNAs) and
messenger RNAs (mRNAs) in EAT derived from patients with coronary artery disease (CAD).

Methods:
EAT samples from 8 CAD, and 8 non-CAD patients were obtained during open-heart
surgery, respectively. The expression of lncRNAs and mRNAs in each EAT sample was investigated
using microarray analysis and further verified using reverse transcription-quantitative polymerase
chain reaction.

Results:
Overall, 1,093 differentially expressed mRNAs and 2,282 differentially expressed lncRNAs
were identified in EAT from CAD vs. non-CAD patients. Analysis using Gene Ontology and
the Kyoto Encyclopedia of Genes and Genomes showed that these differentially expressed genes
were mainly enriched in various inflammatory, immune, and metabolic processes. They were also
involved in osteoclast differentiation, B cell receptor and adipocytokine signaling, and insulin resistance
pathways. Additionally, lncRNA-mRNA and lncRNA-target pathway networks were built to
identify potential core genes (e.g., Lnc-CCDC68-2:1, AC010148.1, NONHSAT104810) involved
in atherosclerotic pathogenesis.

Conclusion:
In summary, lncRNA and mRNA profiles in EAT were markedly different between
CAD and non-CAD patients. Our study identifies several potential key genes and pathways that
may participate in atherosclerosis development.
Comparative Assessment of Polyherbal Formulation and Mucuna pruriens Extract as Neuroprotectant by Using MPTP Screening Mouse Model of
Parkinson’s Disease
Authors: Srivastava Amit Kishor , Naseer Arif , Gupta Amresh 
Published: January 2022

Background:
The main aim of the study was to compare the neuroprotective potential
of Polyherbal Formulation (PHF) with that of an extract of a well-reported anti-parkinson plant,
i.e., Mucuna pruriens.

Methods:
Different PHF combinations (PHFs) were formulated by using hydro-alcoholic extracts
and were tested for neuroprotective potential against Mucuna pruriens extract (MPE). In the experimental
study, 30 albino mice (Swiss strain, 35-45g) were grouped into Control, MPTP, MPTP+
MPE, MPTP+PHFs, MPTP+ L-DOPA groups. Experimental mice were given PHFs and MPE
(50 mg/kg body wt.) by intraperitoneal routes. MPTP (1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine)
was given orally for 2 weeks with prior use of PHFs and MPE 20 mg/kg body wt. for 2
weeks. After treatment, a neurobehavioral study was performed as well as neurochemical parameters
were evaluated.

Results:
The results showed that polyherbal formulation improved the performance of the dopaminergic
neurons in the substantia nigra region of the brain compared to MPE with respect to MPTP intoxication.
A significant reduction was found in spontaneous locomotor activity and rotarod activity
in MPTP treated mice in contrast with the control group, in whom these activities were restored
by MPTP+MPE and MPTP+PHF1; however, this contrasted with the standard L-Dopa treatment
group. This improvement was observed to be significantly better in the MPTP+PHF1 treated group
compared to the treatment group of MPTP+MPE. The changes in different parameters occurred after
the MPTP treatment. These changes were observed in the levels of malondialdehyde (MDA),
conjugated dienes (CD), superoxide dismutase (SOD), and catalase.

Conclusion:
The study concluded that PHF treatment promotes significant neurogenesis, reduces
apoptosis, promotes antioxidant capacity, and restores dopamine levels. PHF contains numerous
classes of chemical constituents, which show a synergistic effect for better therapeutic remuneration
and neuroprotection compared to the single chemical entity L-DOPA, which is a well-known
chemical constituent present in MPE.
ADR Profile of the Covishield Vaccine Among Healthcare Workers in a
Tertiary Care Teaching Hospital in India
Authors: Deb Tirthankar , Garg Rajesh , Kaur Manmeet , Beniwal Ankita , Gupta Varsha 
Published: January 2022

Background:
Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory
Syndrome Coronavirus 2 (SARS-CoV-2/2019-nCoV). Though many classes of drugs have
been advocated/used for the treatment of this disease, vaccines are being targeted for its ultimate
prevention. Indian Government has approved Covishield (ChAdOx1 nCoV19 Corona Virus
Vaccine-Recombinant) for restricted use in emergency circumstances.

Objective:
This study is aimed at evaluating the safety profile of the Covishield vaccine among
healthcare workers in a tertiary care hospital.

Methods:
All Adverse Drug Reactions (ADRs) reported to the ADR monitoring centre by healthcare
workers who received the Covishield vaccine during the study period of 3 months were analysed
on various parameters, and the assessment was done using the WHO-UMC scale.

Results:
During the study period, a total of 1216 beneficiaries were vaccinated with 1st dose of Covishield,
and out of these, 727 beneficiaries also received 2nd dose of the vaccine. Out of the 1216
beneficiaries vaccinated, 69 ADRs were reported in 24 healthcare workers. The most common
ADR reported was fever (24.64%), followed by myalgia (18.84%). No death or any serious anaphylactic
reaction was observed. None of the healthcare workers experienced any adverse event during
the observation period of 30 minutes. Serious reactions were reported in 4 healthcare workers as
per the Pharmacovigilance Programme of India (PvPI) criteria of serious AEFI. 92% of healthcare
workers recovered from ADRs during the study period. 84% of ADRs were classified as probable
as per the WHO-UMC scale.

Conclusion:
As per the Government of India directives, all the healthcare workers in the institute
were encouraged to take both doses of the Covishield vaccine. The beneficiaries were kept under
observation for 30 minutes and monitored for any adverse drug reaction following vaccination. The
vaccine was found to be safe with a very low incidence of ADRs.
Developing a Mobile Patient-Reported Outcomes Version of the Common Terminology Criteria for Adverse Events Administration System to Capture Postradiation Toxicity in Oncology: Usability and Feasibility Study
Authors: Underwood Jody , McCloskey Susan , Raldow Ann , Kishan Amar , Zalkin Chad , Navarro Daniel , Holt Lisa Scott , Webb Andrew , Lynch Kathleen A , Atkinson Thomas M 
Published: January 2022

Background
Accurate self-reported symptomatic toxicity documentation via the Patient-Reported Outcomes version of the Common Terminology Criteria for Adverse Events (PRO-CTCAE) is essential throughout cancer treatment to ensure safety and understand therapeutic efficacy. However, the capture of accurate toxicities from patients undergoing radiation therapy is challenging because this is generally provided only at the time of scheduled visits.

Objective
This study seeks to establish the usability and feasibility of a mobile PRO-CTCAE Administration System (mPROS) to capture toxicities related to radiation therapy.

Methods
English-speaking adult patients who were undergoing radiation therapy for cancer were enrolled and given a brief demonstration of the Say All Your Symptoms (SAYS) and Symptom Tracking Entry Program (STEP) interfaces of the mPROS app, followed by a patient-use phase where patient actions were observed as they navigated mPROS to enter toxicities. Patient feedback was captured via a semistructured interview and brief questionnaire.

Results
We enrolled 25 patients (age: mean 60.7 years; females: n=13, 52%; White patients: n=13; 52%; non-Hispanic patients: n=19, 76%; college graduates: n=17, 68%). Patients almost equally preferred the SAYS (n=14, 56%) or STEP (n=11, 44%) interfaces, with 21 patients (84%) agreeing that they would use mPROS to report their symptoms to their health care team and 19 patients (76%) agreeing that they would recommend mPROS to others.

Conclusions
The mPROS app is usable and feasible for facilitating the patient reporting of radiation therapy–related symptomatic toxicities. A revised version of mPROS that incorporates patient input and includes electronic health record integration is being developed and validated as part of a multicenter trial.
Extraction of Synovial Fluid from the Non-Effusive Pathologic Knee with Pneumatic Compression
Authors: MD Ahsan Iqbal, , MD Sumir Brahmbhatt, , MD Maheswari Muruganandam, , MD Jaren R. Trost, , MD Fatemeh Jafari Farshami, , MD David R. Cisneros, , MD Adnan N. Kiani, , MD Matthew K. McElwee, , PhD William A. Hayward, , PhD Luke J. Haseler, , PhD Philip A. Band, , MD Wilmer L. Sibbitt, Jr., 
Published: January 2022

Objectives –Aspiration of synovial fluid from non-effusive
joints is undertaken for the diagnosis of crystal-associated arthritis,
biomarker analysis, and to confirm intraarticular positioning. We
hypothesized that pneumatic compression of the non-effusive knee would
mobilize occult synovial fluid and improve arthrocentesis success.
Methods – The absence of a knee effusion was determined by
physical examination, imaging, and exclusion of confounding
disease. Conventional arthrocentesis was performed in 111 consecutive
non-effusive knees and arthrocentesis volume (milliliters) determined.
Pneumatic compression was then applied, and arthrocentesis was resumed.
Results – Pneumatic compression improved fluid yield:
conventional: 0.4±1.0 ml, compression: 1.8±2.5 ml (319% increase, 95%
CI -1.9<-1.4<-0.9; p=0.0001). Pneumatic compression
reduced arthrocentesis failure (< 0.1 ml) from 74.8% (83/111)
to 41.4% (46/111) (p=0.0001) and improved successful arthrocentesis in
terms of adequate synovial fluid yield: 1) ≥ 0.1 ml from 25.2% (28/111)
to 58.5% (65/111) (+132%, p=0.0001), 2) ≥ 0.5 ml from 22.5% (25/111)
to 57.7% (64/111) (+156%, p =0.0001), 3) ≥ 2.0 ml from 11.7% (13/111)
to 47.7% (53/111) (+300%, p =0.0001), and 4) ≥ 3.0 ml from 5.4%
(6/111) to 36.0% (40/111) (+319%, p =0.0001).
Conclusions: Pneumatic compression of the non-effusive knee
improves the extraction of synovial fluid of various requisite volumes
for conventional and biomarker analysis.
Kontrovesi Perang Malam Hari dan Ambivalensi Tokoh Karna dalam Lakon Suluhan
Authors: Lutfhi Muhammad 
Published: January 2022

AbstractThis study aims to understand the meaning of Suluhan or the Death of Gatutkaca Play by Ki Hadi Sugito. This study employs qualitative descriptive method in which Paul Ricoeur's hermeneutic theory is used. Note-taking, listening, and identity techniques were used in data analysis. The results show that Adipati Karna’s decisions and actions to fight at night becomes the driving force of the events that occur in the next scenes. Adipati Karna's actions break the rules of war to disprove the accusation of being a spy and to prove his loyalty to the Kurawas. The death of Gathutkaca in Tegal Kurusetra is not solely due to the Adipati Karna’s or Kalabendana’s actions, but it has become Gathutkaca’s choice and destiny as it is written in the Jitabsara Book. AbstrakPenelitian ini bertujuan memahami makna Lakon Suluhan Ki Hadi Sugito. Penelitian ini menggunakan teori hermeneutika Paul Ricoeur. Metode deskriptif kualitatif digunakan dalam penelitian ini. Teknik catat, simak, dan padan orthografi digunakan dalam analisis data. Hasil penelitian menunjukkan bahwa keputusan dan tindakan adipati Karna berperang pada malam hari menjadi penggerak dari peristiwa-peristiwa yang terjadi dalam adegan-adegan berikutnya. Tindakan Adipati Karna melanggar aturan perang dalam rangka untuk menegasi tuduhan mata-mata terhadap dirinya dan untuk membuktikan kesetiaannya pada Kurawa. Kematian Gathutkaca di Tegal Kurusetra bukan semata-mata karena perbuatan Adipati Karna atau Kalabendana, namun sudah menjadi pilihannya dan dalam kerangka takdir Tuhan Yang Maha Kuasa seperti tertulis dalam Kitab Jitabsara.
Tiempo y teoría social. Hacia una clasificación operativa de las sociologías del tiempo
Authors: Torres Felipe 
Published: January 2022

El presente trabajo cruza tres niveles desde los cuales se podría abordar la noción ‘tiempo’ en la teoría social. En primer lugar, una dimensión en sentido estricto, relativa a la localización nuclear del concepto al interior de ciertos análisis de la emergente sociedad contemporánea; en segundo lugar, se explora la aplicación diagonal que el concepto ‘tiempo’ posee en algunos lugares de la teoría sociológica, así como una dimensión relacionada con, tercero, la evaluación que esta categoría tiene en sentido normativo. A través de esta clasificación, se espera hacer mención a los programas teóricos más recientes y originales sobre la temporalidad social. Con esto se espera mostrar i) que el tiempo no resulta una categoría marginal al interior de la sociología; ii) que la clasificación propuesta permite organizar de manera esquemática las diferentes aproximaciones teóricas al tiempo desde los estudios sociales y culturales, y iii) que su estudio resulta de gran actualidad, especialmente en relación a las posibilidades descriptivas y límites críticos de los fenómenos temporales al interior de la teoría sociológica.
--
This paper initially crosses three levels from which the notion of ‘time’ in sociology could be approached. Firstly, a dimension in the strict sense, related to the nuclear location of the concept within certain analyses of the emerging contemporary society; secondly, the diagonal application that the concept ‘time’ has in some places of sociological theory is explored, as well as a dimension related to, thirdly, the evaluation that this category has in a normative sense. Through this classification, it is hoped to make mention of the most recent and original theoretical programmes on social temporality. With this we hope to show i) that time is not a marginal category within sociology; ii) that the proposed classification allows us to organise in a schematic way the different theoretical approaches to time in sociology, and iii) that its study is highly topical, especially in relation to the descriptive possibilities and critical limits of temporal phenomena within sociological theory.