bluuebunny/crossref_metadata_2025_split huggingface dataset:
“RSSC-Lineage Multiplex PCR” assay detects and differentiates Ralstonia solanacearum, R. pseudosolanacearum, R. syzygii and the R3bv2 subgroup
Authors: Paudel Sujan , Dobhal Shefali , Lowe-Power Tiffany , Schlub Robert L. , Hu John , Allen Caitlyn , Alvarez Anne M. , Arif Mohammad 
Published: May 2022

Abstract
Bacterial wilt strains in the Ralstonia solanacearum species complex (RSSC) pose serious threats to economically important crops worldwide. In 2014, Safni and co-workers proposed the reclassification of RSSC into three genomospecies: R. solanacearum (Rsol), R. pseudosolanacearum (Rpseu), and R. syzygii (Rsyz). The revision requires the proper identification of strains for diagnostic and epidemiological studies. In response, we developed an inexpensive and user-friendly “RSSC-Lineage Multiplex PCR” that effectively detects plant pathogenic Ralstonia strains in general and also distinguishes between Rpseu, Rsol, Rsyz, and the high-security Select Agent “race 3 biovar 2” subgroup of Rsol, also known as the phylotype IIB-1 potato brown rot pandemic lineage. Genomes were retrieved from the NCBI GenBank database and screened for unique gene regions using OrthoMCL and other comparative genomic approaches. Specific primers were designed for each genomospecies, Ralstonia in general and for “race 3 biovar 2”. AT-rich flaps were added at 5’ position of each primer to optimize the reaction thermodynamics. The specificity was tested in silico using the NCBI GenBank genome database and an in-house database. In vitro specificity and accuracy of the tool was validated with 110 representative Ralstonia strains and 24 strains from other genera. The assay is highly specific, generating neither false positives nor false negatives. Primer set detection limits ranged from 10- to 100-pg. The assay also detected and differentiated strains from naturally and artificially inoculated plant hosts. This tool is highly specific, reliable, and economical for culture characterization, diagnostics, surveys, quarantine decisions, and epidemiological studies.
Prevalence of antibiotic resistance among blood pathogens isolated from routine laboratory specimens at Livingstone Central Hospital in Zambia
Authors: Mwansa Thresa N. , Chanda Warren 
Published: May 2022

Abstract
Introduction. Bloodstream infections (BSI) are a major public health burden with high mortality, and when coupled with antimicrobial resistance, the healthcare costs increase. This study aimed to establish the prevalence of antimicrobial resistance patterns of pathogens isolated from blood cultures at Livingstone Central Hospital (LCH) from 2019 to 2021. Methods. A single-centre laboratory based retrospective study with information collected from electronic laboratory system generated reports on all isolated organisms at LCH microbiology laboratory for a period of 3 years. Results. A total of 765 specimens were processed from January 2019 to December 2021 and only 331 (43.3%) met the inclusion criteria. More specimens from female (61.3%) than males (38.7%), and from out-patient departments (65.9%) than in-patient departments (34.1%) specimens were processed. Amongst the bacteria isolates identified, Escherichia coli (27.2%) was the commonest isolate followed by Enterobacter agglomerans (22.7%), Klebsiella pneumoniae (13%), Klebsiella oxytoca (6.3%), Enterobacter aerogenes (5.4%), Enterobacter cloacae (5.4%), Citrobacter freundii (C4.8%), Serratia marcescens (3.6%), Proteus mirabilis (3.3%), and Staphylococcus aureus (2.7%). Antibiotic susceptibility testing identified the least potent antibiotic as ampicillin (92.9%) followed by co-trimoxazole (82.7%), nalidixic acid (68.3%), penicillin (66.7%), tetracycline (63.5%), and chloramphenicol (50.3%) whereas the most effective antibiotic was imipenem (84.6%) followed by norfloxacin (64.7%) and nitrofurantoin (61.2%). Resistance maybe affected by patient gender and location. Conclusion. Multidrug resistance strains causing BSI are increasing and imipenem is still effective but risk being over-used. This will impact healthcare costs and increase mortality rates at the hospital level.
Demonstrating the Interply of Machine learning and optimization methods for operational planning Decision
Authors: Achamu Gedefaye , Berhan Eshetie , Geremaw Sisay 
Published: May 2022

Abstract
In this paper, the interplaying of machine learning and optimization problems considered as indispensable problem-solving methods. While solving the real-world problem for distinctive aim but with the possibility of interaction, their integration is not as such a long history. Concerning this interplaying, literature summarized from the perspective of challenges in decision making and supply chain. Problem related to determining and forecasting both sales and demand in inventory decision problem based on classical methods is due to the underlying of relying on observational and censored data, which however is believed insufficient and mostly limited to upstream decision making. Inventory problem literature found encouraging in this regard especially related to incorporating side information for consistent decision-making. Paying attention to such side information at the qualitative scale, as the central scheme, and the classical inventory problem considered to predict then optimization demonstration of operational decision-making. With aim of bringing categorical scaled problems amenable to prediction, optimization and even to integration, encoding methods from machine learning and regressor compared at baseline. Consequently, information other than censored data, also become part of decision making process in this work to make decision consistent in one hand and ease at operational level (downstream) on the other hand. Moreover, the approach outperforms the classical state of an art method in terms of operating performance metrics and the cost found is stable.
Virtual Screening of Novel Phytocompound(s) with Potential to Combat
Mycobacterium tuberculosis Infection
Authors: Khare Deepa , Aggarwal Anchal , Sharma Shilpa 
Published: May 2022

Background:
Tuberculosis is a worldwide health concern, and there is an immediate need for
effective therapeutics to inhibit the infection caused by Mycobacterium tuberculosis. The persistent state
of bacteria and the emergence of Multi-Drug Resistance are the two major reasons for the difficulty in
treating tuberculosis.

Objective:
The study aims to identify novel phytocompounds to effectively inhibit Mycobacterium tuberculosis
by targeting the Esx-1 protein, which plays a vital function in the secretion pathway of M. tuberculosis
to successfully disrupt the host cell and cause tuberculosis.

Method:
In the current study, ~500 novel phytocompounds were screened by docking against Esx-1
using AutoDock Vina 4.2 version. The visualization analysis for selected phytocompounds was performed
using Protein-Ligand Interaction Profiler. A comparative study with a well-known drug for tuberculosis,
Rifampicin, was also performed. Moreover, ADMET analysis was performed to check the druggability
and pharmacokinetic parameters of the selected compounds.

Result:
Based on the analysis, cadabicine, an alkaloid produced by Cadaba fruticose (Vizhuthi), Crataeva
nurvala (Varuna) plants, exhibits the best binding affinity of -7.8 Kcal/mol with the active site residues,
Leu 29 and Trp 43, of Esx-1, which are required for the stability of Esx-1 and virulence of M. tuberculosis
in the host cell. ADMET analysis showed that cadabicine exhibits better druggability and
pharmacokinetic parameters than other selected compounds.

Conclusion:
Cadabicine possesses an acceptable binding affinity with the active site of Esx-1 and exhibits
acceptable physicochemical and pharmacokinetic properties, which makes it a potential new drug candidate
for the treatment of tuberculosis.
Investigation of Carbon Nanotube Metal Zinc Oxide Nanoparticle Synthesis
and its Applications
Authors: Ravi T. , Viswanathan K. , Ramachandran D. , Thirugnanasambandam A. 
Published: May 2022

Background:
Carbon nanotubes (CNT) synthesized by CVD method at 950 ℃. CNT and metal ZnO nanocomposites material synthesized by ball mill procedure have been examined. Stability of nanocomposite material has been attained by cationic ion liquid (IL), 1-tetradecyl-3 methylimidazolium chloride, structural morphology material characterized by Fourier transform infrared (FTIR) spectroscopy, Field emission scanning electron microscopy (FESEM), Ultraviolet visible (UV-Vis) spectroscopy. Furthermore, these materials were analyzed by Energy dispersive X-ray spectroscopy (EDX) to study for the percentage of elemental compositions. Current-Voltage (I-V) characterized by electrical properties are showing highly responsive of resistance variation and easy recoverable high sensitivity was the main feature of the CO2 gas sensing properties.

Objective:
Carbon nanotube and zinc oxide nanoparticle combined with different ratio and optimization of coating methods using for sensor applications.

Methods:
It is synthesized with chemical vapour deposition method and zinc nanoparticle developed by ball mill method. We have optimized spin coating and dip coating method on glass surface.

Results:
FTIR spectrum results are recommended that the existing hydroxyl group and C group CNT-ZnO nanoparticle covered by the surface active site of ZnO. The size and composition of the CNT-ZnO were confirmed by FESEM EDAX studies. The absorption and transmittance wavelength of CNT-ZnO nanoparticle were recorded by UV-Visible spectroscopy. The I-V property shows that the drain current and voltages are varied by gas implying the materials suitable for applications.

Conclusion:
This module can be used to monitor CO¬2 gas application instruments with help of software. In future, this module and techniques can be used to study stress sensor and piezoelectric applications
The Role, Significance, and Association of MicroRNA-10a/b in Physiology
of Cancer
Authors: Elgeshy Khaled M. , Abdel Wahab Abdel Hady A. 
Published: May 2022

Abstract:
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the translation of
mRNA and protein, mainly at the posttranscriptional level. Global expression profiling of miRNAs
has demonstrated a broad spectrum of aberrations that correlated with several diseases, and miRNA-
10a and miRNA-10b were the first examined miRNAs to be involved in abnormal activities upon
dysregulation, including many types of cancers and progressive diseases. It is expected that the
same miRNAs behave inconsistently within different types of cancer. This review aims to provide a
set of information about our updated understanding of miRNA-10a and miRNA-10b and their clinical
significance, molecular targets, current research gaps, and possible future applications of such
potent regulators.
Accounting Estimation Intensity, Auditor Estimation Expertise, and Managerial Bias
Authors: Boone Jeff P. , Khurana Inder K. , Raman K. K. 
Published: May 2022

SYNOPSIS
We examine the relation between the accounting estimation intensity (AEI) ingrained in a company’s financial reports and the company’s propensity to meet or beat analyst earnings forecasts and whether this relation is attenuated by the auditor’s estimation expertise at the city-office or national level. Although we find a positive relation between AEI and the propensity to meet analyst forecasts, we find little evidence to suggest that the relation is weakened by auditor estimation expertise. Along the same lines, we find little evidence to suggest that the positive relation between AEI and audit fees is affected by auditor estimation expertise. Our findings are of potential interest to regulators concerned about insufficient auditor skepticism in the audit of accounting estimates, investors interested in better understanding managerial accounting judgments, and academics investigating audit quality.
JEL Classifications: M41; M42.
Diabetes Care Provider Perceptions Regarding Emerging Adults’ Diabetes Self-Management Influences and Patient-Provider Visit Interactions Within a Safety-Net Hospital
Authors: Wolf Rachel A. , Haw J. Sonya , Martyn Kristy K. , Kimble Laura P. 
Published: May 2022

<p> </p>
<p>The increasing number of emerging adults with diabetes (EAWD) being cared for in adult health care settings requires a better understanding of the needs of EAWD and their interactions with adult health care providers (HCPs). This article describes findings from interviews with endocrinologists and diabetes nurses from a safety-net health care system to investigate HCPs’ perspectives regarding influences on EAWD self-management and HCP interactions with EAWD. HCPs frequently perceived lower EAWD engagement in diabetes management, which was complicated by barriers such as the emotional burden of diabetes, busy lives and multiple responsibilities, and limited access to resources; however, HCPs valued the role of information and communication at visits in tailoring care for EAWD. Measures to tailor care should address the psychosocial burden related to the life stage goals and priorities of EAWD, identification of resources for EAWD and HCPs, and further elucidation of effective self-management guidance and communication strategies to support EAWD in safety-net settings.</p>
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MOVE! Enhanced: A Virtual Multidisciplinary Weight and Diabetes Management Program for High-Risk Veterans. A Feasibility Pilot.
Authors: Mendez Carlos E. , Bertram Kristen , Hansen Jill , Taxman Jill , Feest Aaron , Hanley Alfred , Anton Andreaa L. 
Published: May 2022

<p> </p>
<p>The Clement J. Zablocki Veterans Affairs (VA) Medical Center delivers primary, secondary, and tertiary medical care in 166 acute operating beds and provides >600,000 visits annually through an extensive outpatient program. It is the largest VA facility in Wisconsin and, as part of the VA Integrated Services Network 12 (VISN 12), it is designated as a referral base for the upper peninsula of Michigan and northern Illinois. Throughout the state, the medical center has community-based outpatient clinics in Appleton and Green Bay, serving 11 counties in the north central region; Union Grove, serving four counties in the southeastern region, and Cleveland, serving four counties in the east central region. These programs serve a veteran population of ~250,000 (1)</p>
STUDI KELAYAKAN PENGHAWAAN BANGUNAN WISMA ATLET KEMAYORAN SEBAGAI RUMAH SAKIT DARURAT COVID-19 DENGAN METODE ANALISIS SOFTWARE
Authors: Ayuningtyas Nurina Vidya , Suryabrata Jatmika Adi 
Published: May 2022

Salah satu yang dilakukan pemerintah guna menmbah fasilitas kesehatan ketika angka suspek covid-19 meningkat adalah dengan merubah fungsi bangunan Wisma Atlet Kemayoran milik Kementrian PUPR menjadi Rumah Sakit Darurat Covid 19. Oleh karena itu perlu dilakukan kajian terkait layak tidaknya desain bangunan tersebut untuk membantu proses penyembuhan pasien. Penelitian ini memiliki tujuan untuk mengetahui tingkat kelayakan Wisma Atlet sebagai RS (Rumah Sakit) Darurat COVID. Salah satu faktor yang harus diperhatikan yaitu terkait proses sirkulasi udara yang ada di bangunan tersebut. Metode Penelitian yang digunakan yaitu metode simulasi software. Penghawaan Alami dihitung menggunakan software CFD (Computational Fluid Dynamic) untuk mengetahui aliran udara yang terjadi di dalam bangunan. Dengan melakukan perhitungan ini akan terlihat arah aliran udara yang melewati bangunan apakah terjadi pergantian udara atau cross ventilation ataukah tidak terjadi pergantian udara di dalam ruangan. Berdasarkan hasil simulasi yang didapat dapat disimpulkan bahwa antara model double load dan single load sama-sama memiliki pola pertukaran udara yang karakteristiknya sama. Hanya berbeda pada tingkat kecepatan anginnya.FEASIBILITY STUDY OF AIR CONDITIONING OF KEMAYORAN WISMA ATLET AS A COVID-19 EMERGENCY HOSPITAL USING SOFTWARE ANALYSIS METHODOne of the things the government has done to increase health facilities when the number of suspected COVID-19 increases is to change the function of the Kemayoran Athlete House building belonging to the Ministry of PUPR into a Covid 19 Emergency Hospital. Therefore, it is necessary to conduct a study regarding whether or not the design of the building is appropriate to assist the healing process. This study aims to determine the feasibility level of Wisma Alter as a COVID-19 Emergency Hospital. One of the factors that must be considered is related to air circulation in the building. The research method used is the software simulation method. Penghawaan Alami is calculated using CFD (Computational Fluid Dynamic) software to determine the airflow that occurs in the building. By doing this calculation, the direction of airflow through the building will be seen whether there is a change of air or cross ventilation or whether there is no air change in the room. Based on the simulation result, it can be concluded that the double load and single load models both have the same air exchange pattern with the same characteristics. It only differs in the level of wind speed.
Inovasi Teknologi Pengolahan Limbah Sabut Kelapa di Desa Sigar Penjalin
Authors: Bulkaini  , Sukarne  , Syamsuhaidi  , Dwi Anggara Ristami  , Noviani Adhiningsih  , Dian Maulana  
Published: May 2022

Desa Sigar Penjalin Kecamatan Tanjung merupakan salah satu desa penghasil kelapa di Kabupaten Lombok Utara. Sebelum dijual ke konsumen, kelapa yang dihasilkan dikupas terlebih dahulu sehingga menghasilkan limbah yang disebut sabut kelapa. Sampai saat ini limbah sabut kelapa belum secara maksimal dimanfaatkan oleh masyarakat, sehingga menimbulkan masalah baru yaitu terjadinya pencemaran lingkungan. Hal ini disebabkan karena kurangnya kesadaran dan motivasi masyarakat untuk mengolah limbah serabut kelapa menjadi sesuatu yang bernilai ekonomis. Salah satu solusi yang dapat dilakukan untuk mengatasi permasalahan tersebut adalah pengadaan penyuluhan dan pelatihan pemanfaatan limbah sabut kelapa menjadi barang yang bernilai ekonomis seperti dibuat menjadi pot bunga, dan kotak tempat pensil. Tujuan dari program ini adalah memberdayakan masyarakat Desa Sigar Penjalin dalam memanfaatkan limbah sabut kelapa menjadi barang yang bernilai ekonomis. Metode yang digunakan dalam melaksanakan program adalah metode Participatory Research Appraisal (PRA) yaitu bentuk metode yang melibatkan semua anggota mitra sasaran dalam melakukan melakukan program kerja. Dari program yang telah dilaksanakan, diperoleh hasil bahwa sekitar 75% anggota kelompok ibu-ibu PKK Desa Sigar Penjalin siap untuk mengolah limbah sabut kelapa menjadi barang yang bernilai ekonomis. Hasil monitoring dan evaluasi menunjukkan bahwa pengolahan limbah sabut kelapa menjadi barang yang bernilai ekonomis secara tidak langsung dapat meningkatkan pendapatan ibu-ibu PKK.
Somatotypological features of men of working age – natives of the North
Authors: Averyanova I. V. 
Published: May 2022

Anthropometry is known as the basic method for the body physical status assessment.The aim. The study examined anthropometric and somatometric indices in the working age men and investigated physical development variables specific for the region in terms of being used for early diagnosing obesity-related risks for non-infectious diseases.Materials and methods. One hundred and twenty-three male subjects aged 32–40 participated in the survey (mean age was 35.2 ± 0.2 years). Subjective main physicaldevelopment parameters were analyzed.Results. Participants’ average body length variables were seen to be significantly higher than those in other Russia’s regions and some other countries. The similar tendency was observed for subjective body mass, chest circumference, and body mass index variables with disharmonic somatotype and hypersthenic type of body constitution revealed in examined working age men in comparison with younger male subjects. Musculoskeletal mass loss and fat accumulation indicate the development tendency of sarcopenia in men of working age. Excessive body weight prevalence in 32–40-year-old men was 47 %, and 17 % of the examinees were diagnosed with 1st degree obesity.Conclusion. The survey identified the modern population of male northerners as having negative tendencies in their somatometric picture, which involves disharmonic somatotype, sarcopenia, excessive body weight, and 1st degree obesity and results in significant risks for non-infectious inflammation and cardiovascular diseases at the studied age.
The role of retinal focal photosensitivity in micropulse treatment of vascular genesis focal macular edema
Authors: Guro M. Yu. , Khzardzhan Yu. Yu. , Balalin A. S. 
Published: May 2022

Introduction. Vascular macular edema (ME) is the most common in diabetic retinopathy and retinal vein occlusion. To assess the efficacy and safety of ongoing treatment the dynamics of the best-corrected visual acuity is always taken into account, however, taking into consideration the subjectivity of this indicator, objective methods of assessing the morphofunctional state of the retina should be performed. These include optical coherence tomography and microperimetry.The aim of the study is to evaluate the role of focal retinal photosensitivity (FRP) in assessing the efficacy of micropulse treatment of macular edema of vascular genesis.Material and methods. The study group consisted of 110 patients (110 eyes) including 74 patients (74 eyes) with diabetic macular edema (DME) up to 500 μm and 36 patients (36 eyes) with ME up to 500 μm caused by branch retinal vein occlusion. The patients were divided into two groups randomly: in the first group micropulse was performed using a green laser (λ = 532 nm), in the second group – a yellow laser (λ = 577 nm).Results. In both groups ME decreased significantly to 342 ± 11 µm (M ± σ), maximum corrected visual acuity and FRP increased to 0.59 ± 0.07 and 23.9 ± 1.7 dB (M ± σ), accordingly. Statistically significant difference in treatment efficacy depending on laser length wasn’t detected.Conclusion. FRP in the area of edema of neuroepithelium (NE) has a direct correlation with NE thickness and is an important indicator of the retinal functional state and can be used to develop a personalized approach to patient treatment and assess the effectiveness of ME treatment. Application of micropulse for treatment of vascular genesis focal ME with height up to 500 μm is effective and safe, as it allows to decrease reliably the thickness of NE in the edema zone and improve clinical and functional indices, reliably increase FRP.