bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabarcoding Reveals Diverse Endophytic Fungal Communities in Vaccinium Myrtillus Plant Organs and Suggests Systemic Distribution of Some Ericoid Mycorrhizal and DSE Fungi
Authors: Perotto Silvia , Daghino Stefania , Martino Elena , Voyron Samuele 
Published: January 2022

Abstract
Genome sequencing data revealed unexpected similarities among plant-interacting fungi belonging to different ecological guilds. In particular, the sequenced genomes of ericoid mycorrhizal fungi (ErMF) showed closer similarities with genomes of plant endophytes than with those of other mycorrhizal fungi. ErMF are typically associated with roots of plants in the Ericaceae, but it has never been investigated whether they also colonize other organs of their natural hosts. Here, we applied a metabarcoding approach to describe the fungal community associated with the different organs of Vaccinium myrtillus plants collected in the field. Taxa in the Helotiales and Sebacinales, known to include ErMF, characterize the root endosphere, together with Agaricales and Lecanoromycetes, while the stems were enriched in Agaricomycetes, Tremellomycetes and Pleosporales, the leaves were enriched in Sordariomycetes, Hysteriales, and the flowers were enriched in Dothideomycetes. Operational Taxonomic Units attributed to known or putative ErMF and Dark Septate Endophytic fungi, namely Pezoloma ericae, Meliniomyces spp. and Phialocephala fortinii, were found in all the plant organs. The ErMF Oidiodendron sp. was rarely detected in organs other than roots in field samples, but we could detect its presence in the above-ground organs of V. myrtillus grown in vitro. This first report of ErMF colonizing the above-ground tissues of the host plant mirrors their evolutionary closeness with endophytes and increases the list of fungi found to occupy several niches.
The Economic Effects of Grazing in a Small-Scale Sheep Production System in Central Mexico via an Analysis of Scenarios
Authors: Salinas-Martínez Jesús Armando , Posadas Rodolfo Rogelio , Ángeles-Hernández Juan Carlos , Morales-Díaz Leydi Diana , Rebollar-Rebollar Samuel , Rojo-Rubio Rolando , Arriaga-Jordan Carlos Manuel 
Published: January 2022

Abstract
The objective was to assess grazing as an element of profitability and competitiveness of a small-scale sheep production system (SSSPS) in central Mexico and compare its economic performance by means of an analysis of scenarios. A questionnaire was applied to 66 sheep farmers on technical variables, flock management, economic and social aspects. Two scenarios were analyzed to assess the contribution of grazing on profitability and competitiveness. The first analysis was when grazing was the feed base, and secondly, costs of opportunity and economic impacts were studied under the assumption that sheep do not graze and total feed has to be bought from feed vendors. In order to determine the differences between the farmers with the highest and lowest profitability indexes, a four-stratum stratification was made (stratum 1, between 6 and 35 sheep; stratum 2, 36 to 70 sheep, stratum 3 = 71 - 105 sheep; and stratum 4, 106 to 140 sheep). Quantitative variables (technical management, flock management and social aspects) were evaluated with analysis of variance, while qualitative variables were analyzed by means of a Kruskal-Wallis test. Production cost analysis and profitability index were assessed with the Policy Analysis Matrix. Differences were found between strata, farmers with more than 70 sheep have the best profitability indices and the least vulnerability before the non-grazing scenario. Grazing had a positive effect reducing the cost of production and increasing competitiveness in the four strata assessed. However, farmers with higher technical level, specialized breeds and larger flocks (strata 3 and 4) have higher economic profits.
High Stability and Recyclable Cellulose-based Fluorescent Paper Derived from Waste Bagasse for Anti-counterfeiting
Authors: Guo Lei , Liu Hongchen , Peng Fang , Kang Jian , Haisong Qi 
Published: January 2022

Abstract
Developing fluorescent paper-based materials with recyclable, environmental friendly, excellent stable and uniform performances are still an urgent challenge at present. Herein, a simple, green, and high-efficiency method for the fabrication of high stability and recyclable cellulose-based fluorescent paper from waste bagasse via Hantzsch reaction is proposed. Compared to the pristine paper, the surface modification showed little effect on the morphology, mechanical properties, thermal performance and appearance. Meanwhile, the good writing and printing ability of the paper still remained. The prepared paper showed excellent fluorescence performance, which is very stable even treated with acidic, alkaline, and different solvents for 24 h. It can be processed into different security patterns. What’s more, this strategy could be extended by using other compositions with aldehyde groups. Considering their functional and recyclable features, as well as the convenient and efficient fabrication process, our fluorescent papers hold great potential to be applied in many fields such as anti-counterfeiting.
Synthesis and Characterization Studies of Mesostructured Chitosan Coated Fe3O4 Nanoparticles with Folic Acid
Authors: J Agnes , P Ajith , M Sappani Muthu , D Premanand 
Published: January 2022

Abstract
In this present work, Fe3O4 nanoparticles were obtained by a one-part Co-precipitation method. Secondly, a simple solvothermal method was employed to synthesis Chitosan (CS) coated Fe3O4 nanoparticles. Chitosan was used as a surface modification agent. The surface of Chitosan-coated Fe3O4 nanoparticles was conjugated with Folic Acid (FA). Various properties of the resultant products were performed by characterization studies. The structure and surface morphology of as-prepared nanoparticles were characterized by X-ray diffraction (XRD) and Scanning electron microscopy (SEM). The physical parameter such as strain and the crystallite size was evaluated for all the reflection peaks of the samples by using Williamson–Hall (W-H plot) method. Determination of the chemical component was marked by Fourier transform infrared spectroscopy (FTIR) and Energy-dispersive X-ray spectroscopy (EDAX) analyses. UV- Vis experiment was carried out to record optical absorbance and bandgap energy of the nanoparticles was determined by Tauc’s plot. Thermogravimetric analysis (TGA) was conducted to recognize the thermal stability of the magnetic nanoparticles and endothermic peaks were observed from the first derivative of the TGA curve (DTG curve).
A BDNF-TrkB autocrine loop enhances senescent cell viability
Authors: Anerillas Carlos , Herman Allison , Munk Rachel , Payea Matthew , Rossi Martina , Tsitsipatis Dimitrios , Martindale Jennifer , Piao Yulan , Mazan-Mamczarz Krystyna , Fan Jinshui , Cui Chang-Yi , De Supriyo , Abdelmohsen Kotb , de Cabo Rafael , Gorospe Myriam 
Published: January 2022

Abstract
Cellular senescence is characterized by cell cycle arrest, resistance to apoptosis, and a senescence-associated secretory phenotype (SASP) whereby cells secrete pro-inflammatory and tissue-remodeling factors. Given that the SASP exacerbates age-associated pathologies, some aging interventions aim at selectively eliminating senescent cells. In this study, a drug library screen uncovered TrkB (NTRK2) inhibitors selectively capable of triggering apoptosis of senescent, but not proliferating, human fibroblasts. Senescent cells expressed high levels of TrkB, which supported senescent cell viability, and secreted the TrkB ligand BDNF. The reduced viability of senescent cells after ablating BDNF signaling suggested an autocrine function for TrkB and BDNF, including increased expression of BCL2L2 downstream of BDNF-TrkB, favoring senescent cell survival. Strikingly, treatment with TrkB inhibitors reduced the accumulation of senescent cells in aged mouse organs. We propose that the activation of TrkB by SASP factor BDNF promotes cell survival and could be exploited therapeutically to reduce the senescent-cell burden.
Whole-brain in vivo base editing reverses autistic-like behaviors in mice
Authors: Qiu Zilong , Li Weike , Peng Wanling , Yuan Bo , Yuan Yiting , Wang Jincheng , Han Wenjian , Chen Zhi-Fang , Shan Shifang , Zhu Shujia , Xu Min , Chen Jinlong , Cheng Tian-Lin , Xue Zhenyu 
Published: January 2022

Abstract
Autism spectrum disorder (ASD) is a highly heritable neurodevelopmental disorder associated with deficits in social communication and stereotypical behaviors. Numerous ASD-related genetic mutations have been identified and genome editing methods have been developed but successful genome editing in the whole-brain scale to alleviate autistic-like behaviors in animal models has not been achieved. Here we report the development of a new CRISPR-mediated cytidine base editor (CBE) system, which converts C·G base pairs to T·A. We demonstrate the effectiveness of this system by targeting an ASD-associated de novo mutation in the MEF2C gene (c.104T>C, p.L35P). We constructed a Mef2c L35P knock-in mouse and observed that Mef2c L35P heterozygous mice displayed autistic-like behaviors, including deficits in social behaviors and repetitive behaviors. We programmed the CBE to edit the C·G base pairs of the mutated Mef2c gene (c.104T>C, p.L35P) to T·A base pairs and delivered it via a single dose intravenous injection of blood brain barrier (BBB)-crossing AAV-PHP.eB vector into the mouse brain. This treatment restored MEF2C protein levels and reversed impairments in social interactions and repetitive behaviors in Mef2c L35P heterozygous mice. Together, this work presents an in vivo gene editing strategy in which correcting a single nucleotide mutation in the whole-brain scale could be successfully achieved, further providing a new therapeutic framework for neurodevelopmental disorders.
Community impact on a cryptocurrency: Twitter comparison example between Dogecoin and Litecoin
Authors: Lansiaux Edouard , Tchagaspanian Noé , Forget Joachim 
Published: January 2022

Abstract
Context: The 3rd generation of cryptocurrencies groups together cryptocurrencies as diverse as they are sulphurous, like Dogecoin or Litecoin. While one qualifies as memecoin, the other is of interest to a different category of investors. In our knowledge, no study has independently assessed crypto community economic impact concerning this comparable cryptocurrencies. Method: Our study has retrospectively studied (from 01/01/2015 to 03/11/2021), using open access data, the association strength (using normalized mutual information) as well as the linear correlation (using Pearson’s correlation) between Twitter social networks markers and cryptocurrency economic markers. In addition, we have computed different models in order to forecast past prices values and then we have assessed their precision. Findings and conclusions: While average Dogecoin transaction value is impacted by tweets, Litecoin transactions number and average Litecoin transaction value impacted tweets. Concerning whales, tweets are impacted by Dogecoin whales but any significant relationship was found between Litecoin whales and tweets. According to our ARIMA(0,0,0) models, we have a past values forecasting error of 0.08% on Litecoin and 0.22% on Dogecoin.Therefore, there are thus the beginnings of a scientific rationale in order to build a trading robot based on these big datas. This paper is only for academic discussion, conclusions need to be confirmed by further research.
Prevalence of depression and associated factors among heart failure patients at cardiac follow-up clinics in government teaching hospital at Addis Ababa, Ethiopia; A cross sectional study
Authors: Alemayehu Kassahun , Ayalew Yohannes , Habte Teshome 
Published: January 2022

Abstract
Background
Depression is one of the most co-morbid disorders in patients with heart failure and it is a major public health problem in worldwide. There is great information gap about the depression of heart failure patients in developing countries including in Ethiopia.
Objective
This study aimed to assess prevalence of depression and associated factors among heart failure patients at cardiac follow-up clinics.
Methods
Health care institution based cross-sectional study design was employed to assess the prevalence of depression and associated factors among 424 heart failure patients who were selected by using a systematic random sampling method from January 1 to 30, 2021 at four public hospitals, and the sample was proportionally allocated for each study hospital and then data were collected by using structured-interview questionnaires. Bivariate and Multivariate logistic regression analysis was done to examine the possible predictors and variables with the statistical association of P-value of < 0.05 and a 95% confidence interval were considered.
Result
Data were gathered from heart failure in cardiac follow clinic with 100% response rate. The mean and standard deviation of the age of respondents was 52.68 and17.471years respectively. The prevalence of depression in this study was 59%. According to NYHA class III and IV is highly associated with depression [(AOR:12.8(2.2--71.6),95%CI, P=0.004, (AOR:19.2(1.9-189.9), 95% CI, P=0.011)] respectively, poor self-care behaviours[(AOR: 9.1(2.4-34.6), 95%CI, P=0.001)], alcohol use[(AOR:17.7(4.14-35.65),95% CI,P=0.001)], poor social support[( AOR: 4.6(1.2-16.7),95%CI,P=0.020)], poor knowledge level [(AOR:5.1(1.3-20.6), 95%CI, P=0.020), were associated with Depression and statistically significant.
Conclusion
The evidence from this study indicates that depression is highly prevalent among heart failure patients and age of respondent, alcohol intake, self-care behaviour, social support, knowledge level, and co-morbidity was associated with depression and statistically significant. Therefore, all institutions of cardiac centre should work on screening of depression in heart failure patients, and consult psychiatrists and psychologists for early detection and measures.
An NADPH-auxotrophic Corynebacterium glutamicum recombinant strain and used it to construct L-leucine high-yielding strain
Authors: Chen Sheng-Ling , Liu Ting-Shan , Zhang Wei-Guo , Xu Jianzhong 
Published: January 2022

Abstract
The NADPH-regeneration enzymes in Corynebacterium glutamicum were inactivated to construct an NADPH-auxotrophic C. glutamicum strain by gene knockout and gene replacement. The resultant NADPH-auxotrophic C. glutamicum XL-1 ΔZMICg::ISm (i.e., strain Leu-1) grew well in the basic medium only with gluconate as carbon source. Replacement of the native glyceraldehyde 3-phosphate dehydrogenase (NAD-GapDHCg) by NADP-GapDHCa from Clostridium acetobutylicum is an effective strategy for producing L-leucine in NADPH-prototrophic strain XL-1 and NADPH-auxotrophic strain Leu-1, whereas the L-leucine yield did not differ significantly between these strains (14.1±1.8 g/L v.s. 16.2±1.1 g/L). Enhancing the carbon flux in biosynthetic pathway by recombinant expression plasmid pEC-ABNCE promoted L-leucine production, but the shortage NADPH supply limited the L-leucine yield. The mutated promoters of zwf and icdCg were introduced into C. glutamicum with NADP-GapDHCa and pEC-ABNCE increased L-leucine yield (32.8±1.7 g/L) and improved cell growth (OD562=41.3±4.2) because the resultant strain C. glutamicum XL-1 ΔMICg::ISm GCg::GCa Pzwf-D1 Picd-D2/pEC-ABNCE (i.e., strain Leu-9) exhibited the proper intracellular NADPH and NADH level. This is the first report of constructing an L-leucine high-yielding strain that reasonably supplies NADPH by optimizing the biosynthetic pathway of NADPH from an NADPH-auxotrophic strain.
Long-term nitrogen deposition enhances microbial capacities in soil carbon stabilization but reduces network complexity
Authors: Ma Xingyu , Wang Tengxu , Shi Zhou , Chiariello Nona R. , Docherty Kathryn , Field Christopher B. , Gutknecht Jessica , Gao Qun , Gu Yunfu , Guo Xue , Hungate Bruce A. , Lei Jiesi , Niboyet Audrey , Roux Xavier Le , Yuan Mengting , Yuan Tong , Zhou Jizhong , Yang Yunfeng 
Published: January 2022

Abstract
Background: Anthropogenic activities have increased the inputs of atmospheric reactive nitrogen (N) into terrestrial ecosystems, affecting soil carbon stability and microbial communities. Previous studies have primarily examined the effects of nitrogen deposition on microbial taxonomy, enzymatic activities, and functional processes. Here, we examined various functional traits and interactions of soil microbial communities in a Meditarean-type grassland administrated with 14 years of 7 g m-2 year-1 of N amendment, based on estimated atmospheric N deposition in California, USA by the end of the 21st century. Results: Soil microbial communities were significantly altered by N deposition. Consistent with higher aboveground plant biomass and litter, fast-growing bacteria, based on abundance-weighted average rRNA operon copy number, were favored in N deposited soils. The relative abundances of genes associated with labile carbon (C) degradation (e.g., amyA and cda) were also increased. In contrast, the relative abundances of functional genes associated with the degradation of more recalcitrant C (e.g., mannanase and chitinase) were either unchanged or decreased. Compared with the ambient control, N deposition significantly reduced network complexity, such as average degree and connectedness. The network of N deposited samples contained only genes associated with C degradation, suggesting that C degradation genes became more intensely connected under N deposition. Conclusions: We propose a conceptual model to summarize the mechanisms of how changes in above- and below-ground ecosystems by long-term N deposition collectively lead to more soil C accumulation.
Synthesis of 1,2,3-Benzotriazin-4(3H)-one derivatives as α-glucosidase inhibitor and their in-silico study
Authors: Khalid Zunera , Shafqat Syed Salman , Ahmad Hafiz Adnan , Muzzamil Hafiz , Munawar Munawar Ali , Ahmad Matloob , Asiri Abdullah Mohammad Ahmad , Ashraf Muhammad 
Published: January 2022

Abstract
α-Glucosidase inhibition is considered as an effective strategy for the treatment of diabetes mellitus. Currently, three α-glucosidase inhibitors are being used as drugs; Acarbose, Voglibose and Miglitol. The side effects of these drugs are forcing researchers to search for new and effective molecules. In this research work, novel 1,2,3-benzotriazin-4(3H)-one sulfonamides were synthesized and investigated for their α-glucosidase inhibition activity. TCT: DMF adduct have been utilized for the direct synthesis of targeted sulfonamides. All reactions were performed at room temperature under mild conditions. In-vitro enzyme inhibition studies led us to discover many potent inhibitors demonstrating good to excellent activity. The compound 5c with dimethyl substituent was found to be more potent inhibitor than acarbose with the IC50 value of 29.75±0.14 μM. Compounds 5a, 5b, 5d, 5e, 5f and 5m showed good inhibition results with IC50 value 31.97±0.03, 33.24±0.01, 33.76±1.05, 35.98±0.03, 30.87±0.51 and 37.24±0.04 µM respectively. Further structure activity relationship was analyzed by molecular docking studies.
Dilong Attenuates Pathological Cardiac Hypertrophy Through Akt/mTOR and NF-κB Signaling in Pressure Overload-induced Rats
Authors: Liang Tianming , Zhang Shiyu , Pan Ruiyan , Guo Juan , Sun Tai , Chen Yusha , Zhang Xindi , Xie Wenwen , Liu Ling , Feng Ranran , Zhang Zhijia , Zhou Jin , Zhang Lane 
Published: January 2022

Abstract
Background: Dilong (earthworm), a traditional Chinese medicine obtained from the dried body of Pheretima aspergillum (E. Perrier), has proven cardioprotective effects in cardiovascular disorders. We aimed to investigate the potential therapeutic application of Dilong in preventing cardiomyocyte fibrosis and inflammation during cardiac hypertrophy (CH) after pressure overload (PO).Methods: We used the active protein compounds of Dilong (DAPC) to treat CH in vivo and in vitro. Hypertrophic rats were generated by abdominal aortic constriction (AAC)-surgery in male Wistar rats. DAPC (low-dose, 400 mg/kg per day; high-dose, 800 mg/kg per day, intraperitoneal injection [i.p.]) or captopril (50 mg/kg per day, i.p.) were given to rats for 3 weeks post AAC-surgery. Primary neonatal rat ventricular myocytes (NRVMs) isolated from 1-to 2-day-old SD rats were CH induced by phenylephrine (PE, 50 μ M) with or without DAPC (low-dose, 4 mg/mL; high-dose, 8 mg/mL) or captopril (10 μ M). Myocardial hypertrophy was identified by the heart weight index. Left ventricle remodeling and fibrosis of hypertrophic rats were evaluated by hematoxylin and eosin (H&E) staining, Mason staining, and Wheat germ agglutinin (WGA) staining. Using quantitative real-time PCR to examine the expressions of hypertrophic markers and inflammatory cytokines at mRNA levels. Additionally, protein expression of the Akt/mTOR and NF-κB signaling pathways were evaluated by western blot assay. Results: DAPC markedly decreased heart weight index and improved pathological morphology of the myocardium in AAC-induced rats. Of note, DAPC inhibits hypertrophic biomarkers ANP and BNP in vivo and in vitro. Meanwhile, the levels of IL-1β, IL-6, and TNF-α were decreased. Interestingly, DAPC inhibited the activation of Akt/mTOR and NF-κB proteins, which were in line with the in vitro findings.Conclusion: DAPC treatment in rats with PO-induced CH prevented cardiac fibrosis and had anti-inflammatory effects via regulation of the Akt/mTOR and NF-κB pathways.
Boundary Control of Stochastic Korteweg-de Vries-Burgers Equations
Authors: Liang Shuang , Wu Kai-Ning 
Published: January 2022

Abstract
The boundary control problem is considered for stochastic Korteweg-de Vries-Burgers equations. First, a boundary controller is proposed, and a criterion is obtained for mean square exponential stability by using Lyapunov functional method and inequality techniques. Then, when there exist uncertainties in the system parameters, the robust mean square exponential stability is considered, and a sufficient criterion is obtained. Furthermore, if there are also additive noises in the considered system, the H-infinity performance is investigated and a sufficient condition is obtained to ensure the mean square H-infinity performance. Numerical examples illustrate the validity of the theoretical results.