bluuebunny/crossref_metadata_2025_split huggingface dataset:
The pharmacological mechanisms of Sophoraflavescens in the treatment of atopic dermatitis based on pharmacology network strategy
Authors: Song Xue , Bao Shentao , Yan Ying , Xu Guomei , Zhang Jing , Wang Yunong , Wang Chengxiang , Sun Zhanxue 
Published: March 2022

Abstract
Background
Network pharmacology explores the protective mechanism of Sophoraflavescens on atopic dermatitis (AD).
Methods
With the help of the Traditional Chinese Medicine System Pharmacology Technology Platform (TCMSP) and the Swiss target prediction database, the effective ingredients and potential target information of the active ingredients of Sophoraflavescens were obtained, and the human gene database (GeneCards) software was used to screen out the target genes related to atopic dermatitis. Using network pharmacology to explore possible molecular mechanisms.
Results
A total of 38 active ingredients and 508 corresponding targets of Sophoraflavescens were screened out, of which the core target is PAR-2. Sophoraflavescens and cetirizine can significantly improve the degree of inflammatory cell infiltration in AD model mice, reduce pruritus symptoms and pathological damage, and reduce the levels of PGE2, CGRP, LTB4 and SP. Inhibit the expression of PAR-2 and the downstream receptor TrK-A protein.
Conclusions
Sophoraflavescens and its components can affect AD pruritus by inhibiting the PAR-2 signaling pathway.
Sinonasal hemangiopericytoma: two case reports and review of literature.
Authors: Meherzi abir , Khalifa Mouna , Omri Malika , Belakhdher Mouna , Hwass Jihen , Kermani Wassim , Abdelkefi Mohamed 
Published: March 2022

Abstract
Hemangiopericytoma is a rare tumor that arises from pericytes, classified as a low-grade malignant vascular tumor. It is characterised by a poorly clinical presentation and histologically, often confused with other fibrous tumors, which could pose difficulties on the diagnosis.
The nasal cavity is an uncommon site. The rarity of this tumor imply the absence of management guidelines. So far, it still controversial regarding wether the endonasal surgery could control the tumor resection despite the wide performance, nowdays, of endoscopic techniques. It still unclear also conserning the use of embolization before surgery removal and the place of chemotheray. The high rate of recurrence impose long-term follow-up. So that, we report our experience through two cases and we aimed to discuss the clinical features and the differents treatement procedures. In fact, they are two women who consulted for epistaxis and nasal obstruction. Vascular masses were visualized with nasal endoscopy – one in the left nasal cavity and the second one had a destructive mass of the right hemiface . CT and MRI helped identify the mass, its margins and its extirpability. DiagnosIs was confirmed by histology. In one patient, surgery was performed. The second patient had an agressive and invading tumor for which chemotherapy was performed. The first patient remained free of disease after surgery. The second one had a fatal evolution.
Serum nitric oxide metabolites (NOx) for prognosis of cardiovascular death and acute myocardial infarction in an ESSE-RF case-control cohort with 6.5 year follow up
Authors: Gumanova Nadezhda G. , Bogdanova Natalya L. , Metelskaya Victoria A. , Tarasov Vladimir I. , Kots Alexander Ya. , Kontsevaya Anna V. , Kutsenko Vladimir , Drapkina Oxana M. 
Published: March 2022

Abstract
Aims. The goal of the present case-control study was to assess associations of a panel of routine and experimental biomarkers with the risk for cardiovascular death and acute myocardial infarction (AMI) in a cohort recruited from the multicenter study “Cardiovascular Epidemiology in Russian Federation” (ESSE-RF).Methods and Results. A total of 222 subjects included cardiovascular death (N=48) and AMI cases (N=63) detected during 6.5-year follow up with corresponding numbers of matched healthy controls. The levels of 15 routine and experimental biochemical markers were assayed to analyze associations with the outcomes using logistic and Cox proportional hazard regressions validated by comparison between various groups to assess the estimated hazard ratio (eHR). Elevated levels of cardiac troponin I (cTnI), C-reactive protein (CRP), and metabolites of nitric oxide (NOx) were independently associated (P<0.001) with a higher risk of cardiovascular death in various models with adjusted eHRs ranging from 1.83 to 3.74. Elevated levels of NOx and cTnI were significantly (P<0.001) and independently associated with a higher risk of nonfatal AMI with eHR ranging from 1.78 to 2.67. Elevated levels of angiopoietin-like protein 3 (ANGPTL3) were significantly (P<0.001) and independently associated with a lower risk of cardiovascular death in certain models (eHRs from 0.09 to 0.16) and a higher risk of nonfatal AMI (eHR 2.07; P=0.01).Conclusion. Subsequent large scale validation studies should focus on the investigation of predictive impact of cTnI, NOx, CRP, and ANGPTL3 for the development of nationwide recommendations for individual baseline stratification of patients with potential cardiovascular risks.
TaLBD1, a LOB transcription factor gene in T. aestivum, confers plant adaptation to low-N stress via modulating N acquisition-associated processes
Authors: Zhang Yanyang , Ni Chenyang , Li Tianjiao , Han Le , Du Pingping , Xiao Kai 
Published: March 2022

Abstract
Distinct members of the transcription factor (TF) families act as the critical mediators in plant low-N stress response by regulating transcription of the stress defensive-associated genes. In this study, we characterized TaLBD1, a member of the Lateral Organ Boundary (LOB) TF family in T. aestivum, in modulating plant adaptation to the N deprivation condition. The TaLBD1 protein harbors conserved domains specified by the plant LOB TF proteins, which targeted onto nucleus after sorted from endoplasmic reticulum (ER). The transcripts of TaLBD1 in roots and aerial tissue were shown to be response to modified external N levels, showing in the tissues with a pattern to be gradually upregulated following a 27-h N starvation treatment. Transgene characterization on TaLBD1 in N. tabacum indicated that it confers plants improved phenotype, root system architecture (RSA) establishment, dry mass production, and N accumulation under N starvation (NS) condition. Analyses on expression patterns of the genes in nitrate transporter (NRT) and those in PIN-FORMED (PIN) families revealed that the NRT gene NtRNT2.4 and PIN gene NtPIN6 displayed significantly upregulated expression in the NS-challenged lines. Further knockdown expression of them indicated that NtNRT2.4 regulates N uptake whereas NtPIN6 modulates RSA behavior. These results suggested that the TaLBD1-mediated plant NS adaptation is associated with its role in transcriptionally regulating distinct NRT and PIN members that improve N uptake and RSA establishment of plants upon low-N stress. High throughput RNA-seq analyses indicated that a quantity of genes modified expression upon NS underlying TaLBD1 regulation. These differentially expressed (DE) genes were enriched into the gene ontology (GO) terms associated with signal transduction, transcription, protein biosynthesis, primary or secondary metabolism, and stress defensiveness. Our results together suggested that TaLBD1 acts as one of the crucial regulators in plant adaptation to low-N stress. It can be used as a valuable index for evaluating plant NS response and a potential target for molecular breeding of high N use efficiency (NUE) crop cultivars.
Metformin reverse minocycline to inhibit minocycline-resistant Acinetobacter baumannii by destroy the outer membrane and enhance membrane potential in vitro
Authors: Guo Tingting , Sun Xiaoli , Yang Jie , Yang Liying , Li Mengying , Wang Yuhang , Jiao Hongmei , Li Guocai 
Published: March 2022

Abstract
Background: Acinetobacter baumannii (A. baumannii) is an opportunistic pathogen and has emerged as one of the most troublesome pathogens. Drug resistance in A. baumannii has been reported on a global scale. Minocycline was found to be active against multi-drug resistant A. baumannii and was approved by the FDA for the infections caused by sensitive strains of A. baumannii. However, the emergence of minocycline resistance and its toxic effects still need to be addressed. Therefore, this study aimed to evaluate the synergistic effects of metformin combined with minocycline on minocycline-resistant A. baumannii. Results: The effect of metformin on the antibacterial activity of minocycline was determined by checkerboard and time-kill assays. Further, it was observed by biofilm formation assay that metformin combination with minocycline can inhibit the formation of biofilm. Outer membrane integrity, membrane permeability, membrane potential and reactive oxygen species (ROS) were monitored to explore the underlying synergistic mechanisms of metformin on minocycline. And the results shown that metformin can destroy the outer membrane of A. baumannii, enhance its membrane potential, but does not affect the membrane permeability and ROS.Conclusion: These findings suggested that the combination of metformin and minocycline has the potential for rejuvenating the activity of minocycline against minocycline-resistant A. baumannii.
Planar Pentacoordinate Aluminium and Gallium Atoms
Authors: Yashmin Farnaz , Kalita Amlan J. , Borah Ritam R. , Baruah Indrani , Deka Rinu P. , Guha Ankur K. 
Published: March 2022

Abstract
Planar hypercoordinate structures are gaining immense attention due to the shift from common paradigm. Herein, our high level ab initio calculations predict that planar pentacoordinate aluminium and gallium centres in Cu5Al2+ and Cu5Ga2+ clusters are global minima in their singlet ground states. These clusters are thermodynamically and kinetically very stable. Detailed electronic structure analyses reveal the presence of both σ and π aromaticity which is the driving force for the stability of the planar form.
Statistical Validation of LENS: Towards Understanding the Empty Nest Syndrome in India using Lyfas, an Optical Biomarker-based Smartphone Biomedical Tool
Authors: Chattopadhyay Subhagata , Das Rupam 
Published: March 2022

Abstract
Background
Empty Nest Syndrome (ENS) is not a listed clinical entity in DSM-5, although ICD-11 has listed it under “Other Associated Problems with Social or Cultural Environment”. However, a complex grief state is observed in the parents when their children leave home for study and work or marriage. Literature shows ENS may progress to clinical-grade depression and anxiety, if left uncared.
Methods
The study presents a solution (LENS) to assess such risks in ENS using Cardiovascular Optical Biomarkers (COB) from the capillary blood flow signal of the index finger using the technique of arterial photoplethysmography when the finger is gently pressed on the phone camera with the torchlight ‘on’. The scores of COB provide the psychological and metabolic snapshots of the mind-body homeostasis.
Results
The study observes that (a) COB (e.g., SD1/SD2, LF/HF, HRVScore, and ENERGY), obtained through Lyfas tests, and (b) physical e.g., Diastolic Blood Pressure, BMI, Heart rate, HbA1c, TSH, and Estradiol have significant roles in the backdrop of ENS. Bland Altman's reliability measure shows that LENS (novel instrument under trial) has a high agreement of 92.85% and 93.86%, respectively with ENS scoring done by senior psychologists (champion instrument – 1) and HAM-D grading performed by psychiatrists (champion instrument – 2).
Conclusions
LENS can be used as an assistive tool to screen and monitor ENS cases in a health set up.
ICA1 is differentially methylated and expressed in familial type 1 diabetes
Authors: Dashti Mohammad , Nizam Rasheeba , Hebbar Prashantha , Jacob Sindhu , John Sumi Elsa , Channanath Arshad , Al-Kandari Hessa , Thanaraj Thangavel Alphonse , Al-Mulla Fahd 
Published: March 2022

Abstract
There has recently been a growing interest in examining the role of epigenetic modifications, such as DNA methylation, in the etiology of type 1 diabetes (T1D). This study aimed to delineate differences in methylation patterns between T1D-affected and healthy individuals by examining the genome-wide methylation of individuals from three Arab families from Kuwait with T1D-affected mono-/dizygotic twins and non-twinned siblings. Bisulfite sequencing of DNA from the peripheral blood of affected and healthy individuals from each of the three families was performed. Methylation profiles of the affected individuals were compared to those of healthy individuals. The principal component analysis on methylation profiling based on base-pair resolution clustered the T1D-affected twins together family-wide. Sites/regions that were differentially methylated between T1D and healthy samples harbored 84 genes, of which 18 were known to be differentially methylated in T1D individuals compared to healthy individuals in publicly available gene expression data resources. We further validated two of the 18 genes—namely ICA1 and DRAM1 that were hypermethylated in T1D samples compared to healthy samples—for upregulation in T1D samples from an extended study cohort of familial T1D. The ICA1 is well-known to have a role in the development of autoimmune beta-cell destruction.
Patient-reported outcome (PRO) measurements in chronic and malignant diseases: Ten years’ experience with PRO-algorithm-based patient-clinician interaction (telePRO) in AmbuFlex
Authors: Hjollund Niels Henrik Ingvar , Larsen Louise Pape , de Thurah Annette Ladefoged , Grove Birgith Engest , Skuladottir Halla , Linnet Hanne , Friis Rasmus Blechingberg , Johnsen Søren Paaske , May Ole , Jensen Annesofie Lunde , Hansen Troels Krarup , Taarnhøj Gry Assam , Tolstrup Lærke Kjær , Pappot Helle , Ivarsen Per , Dørflinger Liv , Jessen Anne , Sørensen Nanna Toxvig , Schougaard Liv Marit Valen , Team The AmbuFlex 
Published: March 2022

Abstract
Background Patient-reported Outcome (PRO) measures may be used as the basis for out-patient follow-up. Instead of fixed appointments, patients attend follow-up based on disease-specific questionnaires filled in at home (telePRO). The questionnaire is handled in real time by a disease-specific algorithm, which assigns an outcome color reflecting clinical need. Questionnaires and algorithms are constructed in a consensus process with clinicians. We aimed to describe AmbuFlex’ telePRO solutions and the algorithm outcomes and variation, to discuss differences between patient groups, and to highlight possible applications and challenges. Methods TelePRO solutions with more than 100 processed questionnaires were included in the analysis. Data were retrieved together with data from national registers. Results From 2011 to 2021, 29 disease-specific telePRO solutions were implemented within 24 different ICD-10 groups. A total of 42,015 patients were referred and answered 171,268 questionnaires. An existing disease-specific instrument with cut-off values was available for four solutions, whereas items were selected or developed ad hoc for the other solutions. Mean age ranged from 10.7 years to 73.3 years. Mortality varied between 0 and 528 per 1000 patient years. Variations in algorithm outcomes reflected differences in patient groups and aims while different solutions within the same patient group varied little. Discussion TelePRO can be applied in diseases where PRO can be used to evaluate clinical status and needs. Questionnaires and algorithms should be adapted for the specific patient groups and clinical aims.
Association of Apolipoprotein B /Apolipoprotein A-I Ratio With Cardiometabolic Risk Biomarkers in Type 2 Diabetes Mellitus
Authors: Makaju Hari Sharan , Sharma Vijay Kumar , Yadav Binod Kumar , Tuladhar Eans Tara , Bhandari Roshan , Bhattarai Aseem , Raut Mithileshwor , Dubey Raju , Niraula Apeksha , Sapkota Alisha , Neupane Anant , Ramtel Rabina 
Published: March 2022

Abstract
Background
Patients with Type 2 Diabetes mellitus and metabolic syndrome have increased risk for cardiovascular disease (CVD). Measuring the Apolipoprotein B (Apo B) can accurately estimate the atherogenic particles whereas Apolipoprotein A-I (Apo A-I) is the major structural constituent of antiatherogenic high-density lipoproteins. Early analysis of cardiometabolic risk reduces the chance of CVD.
Objectives
The aim of this study was to evaluate the association of Apo B/Apo A-I ratio with cardiometabolic risk biomarkers in T2DM.
Material and Methods
This cross sectional study conducted at Tribhuvan University Teaching Hospital, included 120 diagnosed with type 2 diabetes Mellitus visiting Medicine OPD and 120 apparently healthy controls. Clinical and anthropometric characteristics were documented using clinical proforma. The fasting blood samples were collected for estimating serum glucose, Apo B, Apo A-I, traditional lipid profile analysis and Apo B/Apo A-I ratio were calculated.
Results
The mean values of cardiometabolic risk biomarkers and Apo B/Apo A-I ratio in patents with T2DM were significantly higher whereas HDL-C was significantly lower than that of a control group (p ≤ 0.001). There was a strong positive correlation between the Apo B/Apo A-I ratio and LDL-C, TC, Non HDL-C, and Apo B (r = 0.68–0.89, p < 0.001). Similarly, modest positive correlation was found with BMI, WC, TG and VLDL-C (r = 0.55–0.67, p < 0.001) and a weak, yet significant, correlation (r = 0.18–0.35 p < 0.05) with weight, systolic blood pressure and FBG. In contrast, the ratio showed negative correlation with HDL-C and Apo A-I (r = -0.58, p < 0.001).
Conclusion:
The present study demonstrated that an elevated Apo B/Apo A-I ratio constituted a good association with cardiometabolic biomarkers and supports that the Apo B/Apo A-I ratio as a promising risk marker of future cardiovascular disease as it is not interfered by dietary variation of lipid intake during investigation.
The efficacy of pegylated interferon and entecavir in HBeAg-positive children and adolescents with chronic hepatitis B
Authors: He Yi , zhou Yingzhi , Wang Huimin , Peng Xiaorong , Chang Yunan , Hu Peng , Ren Hong , Xu Hongmei 
Published: March 2022

Abstract
Background and objectives: Pegylated interferon (peg-IFN) and entecavir (ETV) are both recommended as the first-line antiviral drugs for chronic hepatitis B (CHB) at present. We aimed to compare the efficacy and safety between peg-IFN and ETV initial therapy in children and adolescents with CHB and investigate the potential factors affecting the treatment response during the first 48 weeks. Methods: We retrospectively enrolled 70 treatment-naïve children and adolescents with CHB who received peg-IFN(n=26) or ETV(n=44) as initial therapy and completed 48-week follow-up for data analysis. Blood samples before treatment were collected from 26 patients of the cohort to assess the cytokine profiles. Results: We found that initial peg-IFN therapy results in higher rates of hepatitis B surface antigen (HBsAg) serological response (SR) but lower rates of virological and biochemical response rates compared to ETV at week 48. As for achieving hepatitis B envelope antigen (HBeAg) SR, peg-IFN was comparable to ETV in the univariate analysis and turned out to be better than ETV after adjustment for important baseline factors. We also found that elevated pre-treatment IL-18 level was significantly associated with HBeAg SR, and remained as the only independent factor of predicting HBeAg SR after adjustment for other important factors. No serious adverse effects of the 2 drugs were reported during the 48-week follow-up. Conclusions: comparing to ETV, peg-IFN was superior in achieving HBsAg and HBeAg SR; higher baseline IL-18 levels were independently associated with HBeAg SR in this study of children and adolescents with CHB.
Multi-Scale-Average-Filter-Assisted Level Set Segmentation Model with Local Region Restoration Achievements
Authors: Mabood Lutful , Badshah Noor , Ali Haider , Zakarya Muhammad , Ahmed Aftab , Khan Ayaz Ali , Rada Lavdie , Haleem Muhammad 
Published: March 2022

Abstract
Segmentation of noisy images having light in the background it is a challenging task for the existing segmen-tation models. In this paper, we propose a new variational model for joint restoration and segmentation of noisy images having intensity inhomogeneity in the presence of high contrast light in the background. The proposed model combines statistical local region information of circular regions centered at each pixel with a multi-phase segmentation technique enabling inhomogeneous image restoration. The proposed model is written in the fuzzy set framework and solved by alternating direction minimization method of multipliers. Through experiments, we have tested our proposed model on different types of synthetic and real images in the presence of intensity in-homogeneity and demonstrate the accuracy and the robustness of the proposed model. In addition, the results are compared with state of the art two-phase and multi-phase methods and show that our method has superiority for images in the presence of noise and inhomogeneity.
Maternal Coping Mechanism and Its associated factors following perinatal loss in Hospitals of Wolaita Zone, South Ethiopia, 2021
Authors: Yeshambel Addisu , Alene Tamiru , Asmare Getachew , Asnake Gedion , Anmut Wallilign , Abebe Kelemu 
Published: March 2022

Abstract
Background: Perinatal loss is the most painful and unanticipated experience for those who conceived and it’s a major global concern worldwide, especially in developed countries like Ethiopia. Women who lost their pregnancy are believed to be at higher risk of developing severe grief following a loss. To reduce the risk of complications after perinatal loss, health care providers need to appreciate the grieving process and identify factors of grief in mothers for early copying and to be managed promptly by providing adequate emotional, social, and psychological support. The purpose of this study was to assess Maternal coping mechanisms and its associated factors following perinatal loss in Hospitals of Wolaita Zone, Ethiopia, 2021. Method: A Hospital-based cross-sectional design from June 30/2021-August1/2021 was conducted. Study participants were selected through systematic random sampling. Epi-data manager version 4.6 was used for template preparation and data entry and SPSS version 25 was used for analysis. Logistic regression with 95% CI was employed. The strength of association was declared at a P value<0.05. Result: Out of the total 393 participants, 51.4% fall under high/positive copying whereas the rest 48.6% had a poor coping mechanism following perinatal loss or grief. Participants who have been satisfied on marriage(AOR=5.64, 95% CI(3.10-10.29)), history ANC follow-up(AOR=2.52, 95%CI(1.39-4.57)), profession-based support(AOR=2.91, 95%CI(1.64-5.15)), support from their own spiritual belief (AOR=3.87, 95%CI(2.33-6.43)), support from their parents(AOR=8.11, 95%CI(3.94-16.69)),support from their husband(AOR=3.2, 95%CI(1.74-5.89)) and discussed/ received information from those who had a history of loss(AOR=2.65, 95%CI(1.31-5.32)) were some of the factors associated with maternal copying following perinatal loss. Conclusion and recommendation: Maternal coping following the perinatal loss was relatively low. Thus, Health care providers need to be more cautious and ensure mothers receive adequate care. Increasing awareness of the support needs of perinatally bereaved mothers and ensuring giving adequate emotional, social, and psychological support for them during grieving moments is essential.