bluuebunny/crossref_metadata_2025_split huggingface dataset:
PU.1-c-Jun interaction is crucial for PU.1 function in myeloid development
Authors: Zhao Xinhui , Bartholdy Boris , Yamamoto Yukiya , Evans Erica K. , Alberich-Jordà Meritxell , Staber Philipp B. , Benoukraf Touati , Zhang Pu , Zhang Junyan , Trinh Bon Q. , Crispino John D. , Hoang Trang , Bassal Mahmoud A. , Tenen Daniel G. 
Published: September 2022

AbstractThe Ets transcription factor PU.1 is essential for inducing the differentiation of monocytes, macrophages, and B cells in fetal liver and adult bone marrow. PU.1 controls hematopoietic differentiation through physical interactions with other transcription factors, such as C/EBPα and the AP-1 family member c-Jun. We found that PU.1 recruits c-Jun to promoters without the AP-1 binding sites. To address the functional importance of this interaction, we generated PU.1 point mutants that do not bind c-Jun while maintaining normal DNA binding affinity. These mutants lost the ability to transactivate a target reporter that requires a physical PU.1-c-Jun interaction, and did not induce monocyte/macrophage differentiation of PU.1-deficient cells. Knock-in mice carrying these point mutations displayed an almost complete block in hematopoiesis and perinatal lethality. While the PU.1 mutants were expressed in hematopoietic stem and early progenitor cells, myeloid differentiation was severely blocked, leading to an almost complete loss of mature hematopoietic cells. Differentiation into mature macrophages could be restored by expressing PU.1 mutant fused to c-Jun, demonstrating that a physical PU.1-c-Jun interaction is crucial for the transactivation of PU.1 target genes required for myeloid commitment and normal PU.1 function in vivo during macrophage differentiation.
JWST/MIRI coronagraphic performances as measured on-sky
Authors: Boccaletti A. , Cossou C. , Baudoz P. , Lagage P. O. , Dicken D. , Glasse A. , Hines D. C. , Aguilar J. , Detre O. , Nickson B. , Noriega-Crespo A. , Gáspár A. , Labiano A. , Stark C. , Rouan D. , Reess J. M. , Wright G. S. , Rieke G. , Garcia Marin M. , Lajoie C. , Girard J. , Perrin M. , Soummer R. , Pueyo L. 
Published: September 2022

Context. Characterization of directly imaged exoplanets is one of the most eagerly anticipated science functions of the James Webb Space Telescope. MIRI, the mid-IR instrument, has the capability to provide unique spatially resolved photometric data points in a spectral range never before achieved for such objects.
Aims. We aim to present the very first on-sky contrast measurements of the MIRI coronagraphs. In addition to a classical Lyot coronagraph at the longest wavelength, this observing mode implements the concept of the four-quadrant phase mask for the very first time in a space telescope.
Methods. We observed single stars together with a series of reference stars to measure raw contrasts as they are delivered on the detector, as well as reference-subtracted contrasts.
Results. The MIRI coronagraphs achieve raw contrasts better than 10−3 at the smallest angular separations (within 1″) and about 10−5 farther out (beyond 5 ~ 6″). Subtracting the residual diffracted light left behind the coronagraph has the potential to bring the final contrast down to the background- and detector-limited noise floor at most angular separations (a few times 10−5 at less than 1″).
Conclusions. The MIRI coronagraphs behave as expected from simulations. In particular, the raw contrasts for all four coronagraphs are fully consistent with the diffractive model. Contrasts obtained by subtracting reference stars also meet expectations and are fully demonstrated for two four-quadrant phase masks (F1065C and F1140C). The worst contrast, measured at F1550C, is very likely due to a variation in the phase aberrations at the primary mirror during the observations, and not an issue with the coronagraph itself. We did not perform reference star subtraction with the Lyot mask at F2300C, but we anticipate that it would bring the contrast down to the noise floor.
The Accuracy of Hestia and Simplified PESI to Predict the Prognosis in Pulmonary Embolism: Systematic Review with Meta-analysis
Authors: Palas Miguel , Silva Beatriz Valente , Jorge Cláudia , Almeida Ana G. , Pinto Fausto J. , Caldeira Daniel 
Published: September 2022

AbstractIntroduction Pulmonary embolism (PE) patients at low risk of early complications may be considered for early discharge or home treatment. Last decades evidence has been growing about the safety of several clinical prediction rules for selecting those patients, such as simplified Pulmonary Embolism Severity Index (sPESI) and Hestia Criteria. The aim of this review was to compare the safety of both strategies regarding 30-days mortality, venous thromboembolism recurrence and major bleeding.Methods A systematic literature search was conducted using MEDLINE, CENTRAL and Web of Science on 6th January 2022. We searched for studies that applied both Hestia Criteria and sPESI to the same population. Sensitivity, specificity and diagnostic odds ratio were calculated for both stratification rules. Both Hestia and sPESI criteria of low risk were evaluated to set the number of patients that could be misclassified for each 1000 patients with PE. The estimates were reported with their 95% confidence intervals (95%CI).Results This systematic review included 3 studies. Only mortality data was able to be pooled. Regarding mortality, the sensitivity, specificity and diagnostic odds ratio was 0.923 (95%CI: 0.843–0.964), 0.338 (95%CI: 0.262–0.423) and 6.120 (95%CI: 2.905–12.890) for Hestia Criteria; and 0.972 (95%CI: 0.917–0.991), 0.269 (95%CI: 0.209–0.338) and 12.738 (95%CI: 3.979–40.774) for sPESI score. The negative predictive values were higher than 0.977. The risk of misclassification of high-risk patients in low risk was 5 (95%CI: 3–11) with Hestia and 2 (95%CI: 1–6) with sPESI, for each 1000 patients with PE in terms of mortality.Conclusion The risk of misclassification of patients presenting with low-risk pulmonary embolism with the intent of early discharge or home treatment with both Hestia Criteria and sPESI score is low and these data supports methods for this purpose.
The 10th Costal Cartilage Graft in Secondary Cleft Rhinoplasty—A Versatile Rib
Authors: Ujam Atheer B. , Vig Navin , Nasser Nasser 
Published: September 2022

AbstractAutologous costal cartilage for rhinoplasty is an excellent graft material providing both volume and quality of cartilage required for nasal reconstruction. Commonly, the 5th, 6th, 7th, and 8th rib costal cartilages have been harvested but other ribs such as the 10th rib are often overlooked and yet offer an excellent graft option for columellar, alar rim, nasal tip, and dorsal augmentation grafts. The use of the 10th rib costal cartilage in patients with complex secondary cleft nasal deformity has scant reference in the literature. We demonstrate our simple and quick 10th rib cartilage harvesting technique and elicit its use in secondary rhinoplasty and cleft lip nasal deformities. The advantages of harvesting the 10th rib cartilage and our long-term results demonstrating ease of harvest, stability, and predicable outcomes are with several clinical cases. The senior author has used the 10th rib graft successfully in a variety of challenging secondary cleft rhinoplasty procedures with excellent and consistent outcomes. We advocate the use of the 10th rib graft in such procedures as it is easy to harvest, is a dynamic cartilage graft, and offers predictable results with low levels of complications.
Imaging of Metabolic Bone Diseases: The Spine View, Part I
Authors: Bazzocchi Alberto , Isaac Amanda , Dalili Danoob , Fotiadou Anastasia , Kariki Eleni P. , Kirschke Jan S. , Krestan Christian R. , Messina Carmelo , Oei Edwin H.G. , Phan Catherine M. , Prakash Mahesh , Sabir Nuran , Tagliafico Alberto , Aparisi Francisco , Baum Thomas , Link Thomas M. , Guglielmi Giuseppe , Aparisi Gómez Maria Pilar 
Published: September 2022

AbstractMetabolic bone diseases comprise a wide spectrum. Of them, osteoporosis is the most frequent and the most commonly found in the spine, with a high impact on health care systems and on morbidity due to vertebral fractures (VFs).This article discusses state-of-the-art techniques on the imaging of metabolic bone diseases in the spine, from the well-established methods to the latest improvements, recent developments, and future perspectives.We review the classical features of involvement of metabolic conditions involving the spine. Then we analyze the different imaging techniques for the diagnosis, characterization, and monitoring of metabolic bone disease: dual-energy X-ray absorptiometry (DXA) and DXA-based fracture risk assessment applications or indexes, such as the geometric parameters, Bone Strain Index, and Trabecular Bone Score; quantitative computed tomography; and magnetic resonance and ultrasonography-based techniques, such as radiofrequency echographic multi spectrometry. We also describe the current possibilities of imaging to guide the treatment of VFs secondary to metabolic bone disease.
Imaging of Metabolic Bone Diseases: The Spine View, Part II
Authors: Aparisi Gómez Maria Pilar , Isaac Amanda , Dalili Danoob , Fotiadou Anastasia , Kariki Eleni P. , Kirschke Jan S. , Krestan Christian R , Messina Carmelo , Oei Edwin H.G. , Phan Catherine M. , Prakash Mahesh , Sabir Nuran , Tagliafico Alberto , Aparisi Francisco , Baum Thomas , Link Thomas M. , Guglielmi Giuseppe , Bazzocchi Alberto 
Published: September 2022

AbstractMetabolic bone diseases comprise a wide spectrum. Osteoporosis, the most frequent, characteristically involves the spine, with a high impact on health care systems and on the morbidity of patients due to the occurrence of vertebral fractures (VFs).Part II of this review completes an overview of state-of-the-art techniques on the imaging of metabolic bone diseases of the spine, focusing on specific populations and future perspectives. We address the relevance of diagnosis and current status on VF assessment and quantification. We also analyze the diagnostic techniques in the pediatric population and then review the assessment of body composition around the spine and its potential application. We conclude with a discussion of the future of osteoporosis screening, through opportunistic diagnosis and the application of artificial intelligence.
Factors Associated with Pulse Methylprednisolone Treatment Failure in COVID-19-Related Multisystem Inflammatory Syndrome in Children (MIS-C)
Authors: Sugunan Sheeja , Bindusha S. , Niyas H. R. , Geetha S. , Chinchilu R V 
Published: September 2022

Abstract
Objective This article determines the occurrence and variables associated with pulse methylprednisolone treatment failure in children with coronavirus disease 2019 (COVID-19)-related multisystem inflammatory syndrome in children (MIS-C).
Methods This prospective observational study was undertaken at a tertiary care teaching hospital in Kerala, India. Children admitted with COVID-19-related MIS-C who were treated with pulse methylprednisolone as first-line therapy were included in the study. Depending on the response to the treatment, they were divided into two groups. The clinical, laboratory parameters, and follow-up findings at 3 months were compared between the two groups
Results Seventy-six patients were admitted with MIS-C during the study period. Sixty received pulse methylprednisolone as the first-line therapy. Of the 60 patients who received pulse methylprednisolone, 50 responded to treatment, while 10 required repeat immunomodulation. Need for noninvasive or invasive ventilation (relative risk [RR]: 13.14, 95% confidence interval [CI]: 3.147–54.88), six or more organ involvement (RR: 4.667, 95% CI: 1.349–16.149), thrombocytopenia (RR: 6.43, 95% CI: 0.87–47.6, p 0.003), and abnormal chest X-ray findings at admission (RR: 4.5, 95% CI: 1.46–13.8), were found to be associated with increased risk of treatment failure with pulse methylprednisolone therapy. Note that 88% of patients with coronary artery involvement showed resolution at 3-month follow-up.
Conclusion More than 80% of children with MIS-C can be treated successfully with corticosteroids. The need for ventilator support, abnormal chest X-ray findings, and thrombocytopenia at admission were found to be factors associated with pulse methylprednisolone treatment failure.
Evaluation of Cognitive Domain in Objective Exam of Physiotherapy Teaching Program by Using Bloom's Taxonomy
Authors: Zahoor Al-Wardha , Farooqui Sumaira Imran , Khan Amna , Kazmi Syed Abid Mehdi , Qamar Naveed , Rizvi Jaza 
Published: September 2022

Abstract
Objective For the development and growth in conceptual understanding of education, evaluation is one of the key factors of it. Improving a student's cognitive level is highly dependent upon the questions being asked in exams. The primary aim of this study is to analyze the cognitive level of physiotherapy exam papers using Bloom's taxonomy.
Material and Methods The study was performed in a Private Medical University, Doctor of Physical Therapy Program in all 5 years of mid-term examination of 2019. One thousand and eighty multiple-choice questions were evaluated on revised Bloom's taxonomy of cognitive domain.
Results It was found that most lower order cognitive questions were asked from first- and second-year students, whereas third- to fifth-year students were asked higher order cognitive questions ranging from 27.5 to 38%.
Conclusion The examination analyzed the efficacy of education being provided. It helped in finding the subject content that needs greater emphasis and clarification. The faculty should give consideration on higher order cognitive level questions to encourage critical thinking among students and the medical colleges should develop the policy on construction of question papers according to the goal of each study year.
Fatigue among Cancer Patients Receiving Radiation Therapy in a Selected Hospital, Mangalore
Authors: Raju Giya , Crasta Glinivia Reesha , Rodrigues Jane , Johny Jelmol , Dsouza Jelvita , T. Denitha 
Published: September 2022

Abstract
Introduction Cancer is a group of diseases characterized by the uncontrolled proliferation of aberrant cells. Radiation therapy is one of the cancer treatments options. It uses high-energy waves to attack tumor cells, as well as adjacent cells. The majority of cancer patients report feeling tired at the start of treatment or after a few weeks of radiation therapy. It is one of the most disabling and ongoing side effects of anticancer therapy among cancer patients. As a result, cancer-related fatigue can be a troubling symptom for most people getting anticancer treatment which is commonly underdiagnosed by the health care team. This research study aids us in assessing fatigue in cancer patients who receive radiation therapy as a treatment.
Materials and Methods A quantitative descriptive research design was used in this study to collect data from 138 cancer patients who were undergoing radiation therapy both inpatient and outpatient departments of the selected Medical College Hospital in Mangaluru. The samples for this study were chosen using a purposive sampling technique. Data were collected by administering baseline proforma and Fatigue Assessment Scale.
Results Majority of the patients were experiencing substantial fatigue, that is, 105 (76.08%) of patients were experiencing fatigue, 8 (5.79%) were experiencing extreme fatigue. and only 25 (18.11%) were experiencing no fatigue. Mean percentage shows that the majority, that is, 57%, of the patients with cancer experience physical fatigue and 50.25% experience mental fatigue. There is no significant association found between fatigue and selected demographic variables except for marital status (p = 0.015). Hence H0 is accepted at 0.05 level of significance, except for one selected variable.
Conclusion The present study concluded that fatigue is one of the common symptoms and adverse effects experienced among cancer patients who receive radiation therapy. Health care professionals should consider the impact of fatigue on the individual and effective fatigue management to be provided to improve the quality of life
The Study of Spray-Freeze-Drying Technique for Development of Novel Combination pMDIs, Part I: Study on the Preparation Method
Authors: Xi Quan , Miao Jia-Ying , Cao Zhen , Wang Hao 
Published: September 2022

AbstractClinically available pressurized metered-dose inhalers (pMDIs) mainly directly use micronized drugs as inhalable microparticles. Although technology for preparing pMDIs has proven to obtain clinically appropriate aerosol performance, the fine particle fraction and delivered dose content uniformity (DDCU) of pMDIs still need to be improved. DDCU problem is usually exacerbated by patients' handling errors prior to taking a dose. In this study, novel phospholipid microparticle inhalation pMDIs were prepared by a spray-freeze-drying process using mometasone furoate and formoterol fumarate dihydrate as model drugs and distearoylphosphatidylcholine as an excipient. Combined with the material composition, the atomization and freeze-drying processes were also studied. Our data showed that both atomization parameters of gas–liquid ratio and freeze-drying curve settings met the requirements of drug design. According to aerodynamic performance in vitro and DDCU evaluation, the performance of the phospholipid microparticle inhalation pMDI was better than that of the micronized drug microparticle pMDI. In conclusion, preparing pMDIs with particle engineering has the potential to ensure accuracy of quantification and to improve the efficiency of drug deposition in lungs in clinical practice.
Social stability risk assessment and economic competitiveness in China
Authors: Zhang Ruilian , Worden Sandy , Xu Junzhuo , Owen John R. , Shi Guoqing 
Published: September 2022

AbstractSocial stability risk assessment (SSRA) has become the mainstream policy instrument for assessing potential risks of large-scale development projects across all sectors in China. In this paper, fuzzy qualitative comparative analysis (fsQCA) is used to quantify the impact of SSRA policy on economic competitiveness across China’s 31 provinces using a SSRA policy dataset (2003–2020) and a provincial economic competitiveness dataset (2019–2020). QCA combines Boolean algebra and set theory to identify configurations of conditions that are necessary or sufficient for a given outcome. Rather than following the mainstream statistical method of developing a single causal model that best fits the data, QCA explores multiple concurrent causality. A typology of SSRA policies was developed to guide our analysis. The research concluded that to support high economic competitiveness within provinces, SSRA policies must be structured around solving social stability problems and addressing a specific industry issue (e.g., pollution) in a particular industry (e.g., resources). Policies that only include one of these factors or that focus on the performance of government officials were found to contribute to low economic competitiveness. Reorienting the focus of SSRA policies could support more rigorous risk assessments and enhance economic competitiveness, particularly in provinces that host large-scale development projects. These findings have implications for China’s policymakers given their dual objectives of driving economic reform while maintaining a harmonious society.
Characterising the woody vegetation in contrasting habitat types in the lower Fitzroy River, Western Australia
Authors: Freestone Fiona L. , Canham Caroline A. , Setterfield Samantha A. , Douglas Michael M. , Beesley Leah S. , Loomes Robyn C. 
Published: September 2022

Context Riverine systems consist of distinct habitats along a landscape gradient and characterising the composition and structure of vegetation in these habitats can support environmental water-management decisions. However, in many regions, including northern Australia, there is a paucity of hydro-ecological data. Aims We aimed to characterise the species composition and the structure of riparian and floodplain woody vegetation of the lower Fitzroy River. Methods We surveyed woody vegetation in different habitats within the riparian zone and floodplain. Multivariate analysis was used to assess differences in the composition of riparian woody species among the four habitat types and univariate analysis was used to compare vegetation structure, recruitment, and environmental variables among habitats. Key results The composition and the physical structure of woody species differed among habitat types of the lower Fitzroy River, indicating a zonation of riparian and floodplain vegetation in response to fluvial processes and water availability. The floodplain was characterised by sparsely distributed Eucalyptus microtheca and a sparse (∼30%) canopy cover. In contrast, the riverbank habitat type had very large trees (mean basal area = 0.26 m2), with a dense canopy cover (∼80%) and was dominated by Melaleuca argentea, M. leucadendra and Barringtonia acutangula. Both the top of bank and off-channel wetlands represent a more intermediary environment, characterised by greater species richness and greater seedling recruitment. Conclusions Identifying these habitat types and characterising their physical and biological properties, such as the relationship between flooding and the composition of woody species, provides a framework to assist the management of large floodplain river systems.
Microscope instrument in-flight characterization
Authors: Chhun Ratana , Hardy Emilie , Rodrigues Manuel , Touboul Pierre , Métris Gilles , Bergé Joel , Boulanger Damien , Christophe Bruno , Danto Pascale , Foulon Bernard , Guidotti Pierre-Yves , Huynh Phuong-Anh , Lebat Vincent , Liorzou Françoise , Robert Alain 
Published: September 2022

Abstract
Since the MICROSCOPE instrument aims to measure accelerations as low as a few 10−15 m s−2 and cannot operate on ground, it was necessary to have a large time dedicated to its characterization in flight. After its release and first operation, the characterization experiments covered all the aspects of the instrument design in order to consolidate the scientific measurements and the subsequent conclusions drawn from them. Over the course of the mission we validated the servo-control and even updated the PID control laws for each inertial sensor. Thanks to several dedicated experiments and the analysis of the instrument sensitivities, we have been able to identify a number of instrument characteristics such as biases, gold wire and electrostatic stiffnesses, non linearities, couplings and free motion ranges of the test-masses, which may first impact the scientific objective and secondly the analysis of the instrument good operation.