bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zinc transporter ZIP13 is involved in myogenic differentiation: establishment of Ehlers– Danlos syndrome spondylodysplastic type 3 induced pluripotent stem cells
Authors: Shoji Masaki , Ohashi Takuto , Nagase Saki , Ichihashi Kenta , Takagishi Teruhisa , Nagata Yuji , Nomura Yuki , Hara Takafumi , Yoshigai Emi , Fukunaka Ayako , Fujitani Yoshio , Sakurai Hidetoshi , Santos Heloísa G. dos , Fukada Toshiyuki , Kuzuhara Takashi 
Published: December 2022

Abstract
Ehlers–Danlos syndrome spondylodysplastic type 3 (EDSSPD3, OMIM 612350) is an inherited recessive connective tissues disease caused by loss of function of SLC39A13/ZIP13, a zinc transporter belonging to the Slc39a/ZIP family. Patients with EDSSPD3 suffer from impaired development of bone and connective tissues, and muscular hypotonia, or myopathy. However, whether ZIP13 participates in the early differentiation process of these cell types remains unclear. In this study, we investigated the role of ZIP13 in myogenic differentiation using murine myoblast cell line (C2C12) as well as human patient-derived induced pluripotent stem cells (iPSCs). We found that ZIP13 expression was upregulated by myogenic stimulation in C2C12 cells, and its knockdown disrupted myotubular differentiation. Myocytes differentiated from iPSCs of patients with EDSSPD3 (EDSSPD3-iPSCs) exhibited incomplete myogenic differentiation. Moreover, the phenotypic abnormalities of EDSSPD3-iPSC-derived myocytes were corrected by genomic editing of the pathogenic ZIP13 mutation, suggesting the indispensable role of ZIP13 in myogenic differentiation. These results clearly indicate that ZIP13 is required for proper myogenic differentiation and that the study of EDSSPD3-iPSCs may help shed light on the molecular basis underlying various clinical features caused by the loss of ZIP13.
Electronic Optimization and Modification of Efficient Ir Clusters Embedded onto Ni-Mo-P for Electrocatalytic Oxygen Evolution Reaction
Authors: Wei Hehe , Li Jing , Du Peng , Zhang Longtao , Su Zixiang , Li Hui , Lei Ming , Huang Kai , Gong Xueqing 
Published: December 2022

Abstract
The implementation of low-cost and efficient electrocatalysts for water oxidation is crucial for the development of industrial water electrolysis, however, they often suffer from inferior activity or poor stability. Herein, we demonstrated 0.7-nm iridium clusters embedded onto Ni-Mo-P (Ir-NMP) that exhibited an ultralow overpotential of 290 mV vs reversible hydrogen electrode (RHE) to reach the current density of 50 mA cm− 2 in 1 M KOH, together with low Tafel slop of 35.3 mV dec− 1, high mass activity of 2604 A gIr−1 and excellent catalytic stability with almost complete retention of activity within more than 30 hours. According to characterizations and analysis, the interlaced crystalline and amorphous structure of Ni-Mo-P made homogeneous embedment of ultrathin Ir clusters onto NMP substrate, and the introduction of Ir clusters enabled the optimization of electronic structure for Ni and Mo species in NMP, which were available to highly efficient and durable Ir-NMP electrocatalyst for OER.
Quasi-seamless stitching for large-area micropatterned surfaces enabled by Fourier spectral analysis of moiré patterns
Authors: Kim Woo Young , Seo Bo Wook , Lee Sang Hoon , Lee Tae Gyung , Kwon Sin , Chang Won Seok , Nam Sang , Fang Nicholas , Kim Seok , Cho Young Tae 
Published: December 2022

AbstractThe main challenge in preparing a flexible mold stamp using roll-to-roll nanoimprint lithography is to simultaneously increase the imprintable area with a minimized perceptible seam. However, the current methods for stitching multiple small molds to fabricate large-area molds and functional surfaces typically rely on the alignment mark, which inevitably produces a clear alignment mark and stitched seam. In this study, a self-pattern alignment (SPA) method inspired by moiré engineering is proposed to fabricate scalable functional surfaces and flexible imprint molds with quasi-seamless and alignment mark-free patterning, which is based on the Fourier spectral analysis of moiré patterns formed by superposed identical patterns. By harnessing the rotational invariance property in the Fourier transform, SPA was confirmed to be a simple and efficient method for extracting the rotational and translational offsets in overlapped periodic or nonperiodic patterns with a minimized stitched region, thereby allowing for the large-area and quasi-seamless fabrication of imprinting molds and functional surfaces, such as liquid-repellent film and micro-optical sheets, that surpass the conventional alignment and stitching limits and potentially expand their application in producing large-area metasurfaces.
A Conceptual framework of Cognitive-Affective Theory of Mind: Towards a Precision Identification of Mental Disorders
Authors: Zhou Peng , Ma Huimin , Zou Bochao , Zhang Xiaowen , Zhao Shuyan , Lin Yuxin , Wang Yidong , Feng Lei , Wang Gang 
Published: December 2022

AbstractTo explore the minds of others, which is traditionally referred to as Theory of Mind (ToM), is perhaps the most fundamental ability of humans as social beings. Impairments in ToM could lead to difficulties or even deficits in social interaction. The present study focused on two core components of ToM, the ability to infer others’ beliefs and the ability to infer others’ emotions, which we refer to as cognitive and affective ToM respectively. Charting both typical and atypical trajectories underlying the cognitive-affective ToM promises to shed light on the precision identification of mental disorders, such as depressive disorders (DD) and autism spectrum disorder (ASD). However, most prior studies failed to capture the underlying processes involved in the cognitive-affective ToM in a fine-grained manner. To address this problem, we propose an innovative conceptual framework, referred to as visual theory of mind (v-ToM), by constructing visual scenes with emotional and cognitive meanings and by depicting explicitly a four-stage process of how humans make inferences about the beliefs and emotions of others. Through recording individuals’ eye movements while looking at the visual scenes, our model enables us to accurately measure each stage involved in the computation of cognitive-affective ToM, thereby allowing us to infer about potential difficulties that might occur in each stage. Our model is based on a large sample size (n > 700) and a novel audio-visual paradigm using visual scenes containing cognitive-emotional meanings. The obtained differential features among normal people, DD and ASD individuals overcome the subjectivity of conventional questionnaire-based assessment, and therefore could serve as valuable references for mental health applications based on AI-aided digital medicine.
Extraction of normalized Digital Surface Model (nDSM) from LiDAR Data in Forest Inventory Mapping
Authors: Faisal Abdullah-Al- , Afroz Farzana , Kafy Abdulla Al 
Published: December 2022

Abstract
The retrieval of Light Detection and Ranging (LiDAR) data is a complex procedure that necessitates extensive processing in order to develop terrain and surface models and forest structure applications. The gradual acquisition of LiDAR information is required to create Digital Elevation Models (DEM) and Digital Surface Models (DSM). The purpose of the study was to generate topographic DEM and normalized DSM (nDSM) data from LiDAR point cloud and to outline the canopy height extraction procedure in the New Forest region of the United Kingdom. Later, under 21 random enclosures, a demonstration of how the nDSM can be used in forest inventory mapping was discussed. The results show that, of the various interpolation techniques used to generate DEM, IDW had the lowest RMSE value of 0.382. The Digital Terrain Model (DTM) was created using two neighborhood settings (3×3) and (30×30), with the last one showing higher accuracy. In the comparison of different interpolation techniques, Inverse Distance Weighting (IDW) was found to have the lowest RMSE value of 0.382. Finally, within the enclosures, the percentage of no trees (mostly shrubs), canopy height ranged 2-10m, 10-15m, and > 15 was mapped. Each enclosure with 40% of its area covered by trees taller than 15 m was assumed to be harvestable. The study demonstrated detailed algorithm-based LiDAR data extraction and processing, which can be used to explore and forecast terrestrial ecosystems with advanced longitudinal orientation potentialities.
Soil CO2, CH4 and N2O fluxes in urban forests, treed and open lawns in Angers, France
Authors: Künnemann Tom , Cannavo Patrice , Guérin Vincent , Guénon René 
Published: December 2022

Abstract
Urban green spaces (UGSs) are mostly represented by lawns and forests. These UGSs can store carbon in soil and above-ground biomass, potentially modulated by management intensity and vegetation cover (shading, rainfall intercept, litterfall, …). Trees in lawns can create a local microclimate modifying soil biogeochemical cycles affecting in turn greenhouse gas (GHG) emissions. The objective of this study was to assess the effects of trees on microclimate (temperature and moisture) influencing GHG in contrasted UGS types. We monthly monitored (from March to November 2021) and compared soil CO2, CH4 and N2O fluxes simultaneously with surface temperature and moisture in treed lawns, open lawns and urban forests. Lawns included 4 different management intensities including mowing, irrigation and fertilization practices. Temperature was the best predictor of soil respiration in all UGS types studied and was the highest in open lawns. We showed that moisture reflected by the water filled pore space (WFPS) significantly added on variation explanation. The shading of trees strongly decreased soil respiration in treed lawns while soil properties were similar indicating a straightforward effect of lowering temperature. On the contrary, forests deeply changed soil properties as well as decreased soil temperature resulting in the lowest rates of soil respiration. Urban forests are a sink for CH4 throughout the year. Lawns were weak to mitigate CH4 and a source of CH4 in irrigated parks where WFPS overpassed 75%. N2O fluxes were weak probably reflecting the transition already made from mineral to organic fertilization limiting N availability.
Telephone outreach by volunteer navigators: a theory-based evaluation of an intervention to improve access to appropriate primary care
Authors: Haggerty Jeannie , Smithman Mélanie-Ann , Beaulieu Christine , Breton Mylaine , Dionne Émilie , Lewis Virginia 
Published: December 2022

Abstract
Background: Pilot interventions were implemented in five different regions within a Canada-Australia participatory research programme to improve access to primary healthcare for vulnerable populations. In Québec, Canada, a telephone outreach program used volunteer patient navigators to help unattached persons from deprived neighbourhoods attach successfully to a family doctor newly-assigned to them from a centralized waiting list. According to our theory-based program logic model we evaluated the extent to which the volunteer navigator outreach helped patients reach and engage with their newly-assigned primary care team, have a positive healthcare experience, develop an enduring doctor-patient relationship, and reduce forgone care and emergency room use.
Method: For the mixed-method evaluation, indicators were developed for all domains in the logic model and measured in a telephone-administered survey at baseline and three months later to determine if there was a significant difference. Semi-structured with a subsample of 13 survey respondents explored the mechanisms and nuances of intended effects.
Results: Five active volunteers provided the service to 108 persons, of whom 60 agreed to participate in the evaluation. All surveyed participants attended the first visit, where 90% attached successfully to the new doctor. Indicators of abilities to access healthcare increased statistically significantly as did ability to explain health needs to professionals. The telephone outreach predisposed patient to have a positive first visit and have trust in their new care team, establishing a basis for an enduring relationship. Access difficulties, foregone care and use of hospital emergency rooms decreased dramatically after patients attached to their new doctors.
Conclusions: As per the logic model, telephone outreach by volunteer navigators significantly increased patients’ abilities to seek, reach and engage with care and helped them attach successfully to newly-assigned family doctors. This light-touch intervention led to achievement of the intended policy goals for the centralized waiting list to increase population access to appropriate primary care and reducing forgone care and use of emergency rooms.
Leakage reduction of Water Distribution Networks with Clustering
Authors: Huzsvár Tamás , Wéber Richárd , Szabó Marcell , Hős Csaba 
Published: December 2022

Abstract
Nowadays, the rapid growth of operational costs and human resource shortages increase the importance of the leakage reduction, and the pressure management. This paper presents an optimisation procedure placing pressure-reducing valves (PRVs) to minimise the water losses of real-life water distribution networks (WDNs). The number and placement of the valves are optimised from clustering perspective with the Leiden algorithm. The optimal settings of the valves are determined with a differential evolution algorithm, where a compound fitness function is applied. On the one hand, it minimises the leakages, on the other hand, evades the pressure-dependent consumer outages, i.e. it keeps the pressure above the desired. Seven real-life water distribution networks were analysed from the region of Western Hungary using the techniques. Moreover, three of them are analysed in details. The robustness of the achieved leakage reduction with the optimised PRV placement is analysed to the uncertainty of the leakage exponent.
Advancing positive social determinants of health through collective impact
Authors: McCrae Julie S. , Spain Angeline K. 
Published: December 2022

Abstract
Background
Communities across the U.S. and globally confront the challenge of transforming negative social determinants of health (SDOH) into positive ones. To address this complex social problem, collective impact (CI) has promise but has been critiqued for insufficiently challenging structural inequities. Research applying CI to SDOH is limited. This mixed-methods study examined early adoption of CI in the 100% New Mexico initiative that aims to address SDOH population-wide in a state with strong cultural identity and assets but also persistent socio-economic inequality. |
Methods. A web-based survey, interviews and focus groups were conducted with initiative participants in June and July 2021. Survey participants rated agreement with six items assessing CI foundation from the Collective Impact Community Assessment Scale. Interviews and focus groups centered on motivation to engage, progress in model components, CI core conditions, and contextual factors influencing experiences. Surveys were analyzed using descriptive means. Qualitative data were analyzed using thematic analysis, an inductive approach, stratified analyses, and co-interpretation. Results. Fifty-eight participants completed the survey, and 21 participated in interviews (n = 12) and focus groups (n = 9). Survey means were highest related to initiative commitment, and lower regarding shared ownership, having multiple perspectives and voices, and adequate resources. Qualitative results indicated the framework’s cross-sector emphasis helped motivate participation. Participants embraced the focus on leveraging community assets that characterizes CI and the current framework. Counties used unique engagement and visibility strategies including mural projects and book clubs. Participants reported communication challenges across sector teams which influenced feelings of accountability and ownership. Participants did not report challenges lacking relevant, available, and timely data or tension between funder-driven and community-driven desired outcomes, contrasting previous CI research.
Conclusion. Multiple foundational conditions of CI were supported in 100% New Mexico, including evidence for support of the common agenda addressing SDOH, shared measurement framework, and mutually reinforcing activities. Results suggest that efforts to launch CI to address SDOH, which are by nature multi-sector, should include robust communication support for local teams. The use of community-administered surveys to identify gaps in SDOH resource access contributed to ownership and collective efficacy that may portend sustainability; however, relying on volunteers threatens sustainability.
Partial trisomy 21 with or without highly restricted-Down syndrome critical region (HR- DSCR). Report of two new cases and reanalysis of the genotype-phenotype association
Authors: Pelleri Maria Chiara , Locatelli Chiara , Mattina Teresa , Bonaglia Maria Clara , Piazza Francesca , Magini Pamela , Antonaros Francesca , Ramacieri Giuseppe , Vione Beatrice , Vitale Lorenza , Seri Marco , Strippoli Pierluigi , Cocchi Guido , Piovesan Allison , Caracausi Maria 
Published: December 2022

Abstract
Background
Down syndrome (DS) is caused by the presence of an extra copy of full or partial human chromosome 21 (Hsa21). Partial (segmental) trisomy 21 (PT21) is the duplication of only a delimited region of Hsa21 and can be associated or not to DS: the study of PT21 cases is an invaluable model for addressing genotype-phenotype correlation in DS. Previous works reported systematic reanalyses of 132 subjects with PT21 and allowed the identification of a 34-kb highly restricted DS critical region (HR-DSCR) as the minimal region whose duplication is shared by all PT21 subjects diagnosed with DS.
Methods
We report clinical data and cytogenetic analysis of two children with PT21, one with DS and the other without DS. Moreover, we performed a systematic bibliographic search for any new PT21 report.
Results
Clinical and cytogenetic analyses of the two PT21 children have been reported: in Case 1 the duplication involves the whole long arm of Hsa21, except for the last 2.7 Mb, which are deleted as a consequence of an isodicentric 21: the HR-DSCR is within the duplicated regions and the child is diagnosed with DS. In Case 2 the duplication involves 7.1 Mb of distal 21q22, with a deletion of 2.1 Mb of proximal 20p, as a consequence of an unbalanced translocation: the HR-DSCR is not duplicated and the child presents with psychomotor development delay but no clinical signs of DS. Furthermore, two PT21 reports recently published (named Case 3 and 4) have been discussed: Case 3 has DS diagnosis, nearly full trisomy for Hsa21 and a monosomy for the 21q22.3 region. Case 4 is a baby without DS and a 0.56-Mb duplication of 21q22.3. Genotype-phenotype correlation confirmed the presence of three copies of the HR-DSCR in all DS subjects and two copies in all non-DS individuals.
Conclusions
The results presented here are fully consistent with the hypothesis that the HR-DSCR is critically associated with DS diagnosis. No exception to this pathogenetic model was found. Further studies are needed to detect genetic determinants likely located in the HR-DSCR and possibly responsible for core DS features, in particular intellectual disability.
Molecular Characterization of Metal Tolerant Bacteria Isolated from Contaminated Soil
Authors: Mairaj Asma , Saeed Muhammad Faran , Sarwar Qurat-ul-Ain 
Published: December 2022

Abstract
Soil pollution with toxic metals is an alarming threat to the agricultural sector as these are non-biodegradable. The problem is getting worse in developing countries like Pakistan. Several methods remove the toxic metals from the environment including physical, chemical, and biological processes. Physical and chemical methods are not only expensive but also produce secondary by-products that may damage the ecosystem. Bioremediation is a cost-effective method that reduced environmental pollution by using biodegradation processes. Therefore, this study was carried out for the isolation and characterization of metal tolerant bacteria from wastewater irrigated soil in Multan. Contaminated soil samples were collected from wastewater irrigated soil, and were inoculated on Nutrient agar poured with hexavalent chromium. Positive cultures were freshed on Nutrient agar on basis of colony morphology, and pure colonies were subjected to varying concentrations of metal to determine the Minimum Inhibitory Concentration (MIC). The broth dilution method was used for the determination of minimum inhibitory concentration. Out of four soil specimens, a total of six bacteria belonging to the two different species were isolated and identified on basis of gram staining and biochemical characterization. Differentiation of gram-positive rods on basis of colony morphology and biochemical tests were identified as Bacillus subtilis (n = 4) and Bacillus cereus (n = 2). Gene amplification through specific primers for 16S rDNA was performed according to the protocol. For further evaluation and sequencing, isolates have been sent to Macrogen (Korea).
The effects of high plasma levels of Aβ 1-42 on mononuclear macrophage in mouse models of Alzheimer’s disease
Authors: Li Chunrong , Liu Kangding , Zhu Jie , Zhu Feiqi 
Published: December 2022

Abstract
Microglia plays a crucial role in the pathogenesis of Alzheimer’s disease (AD). Plasma Aβ1−42 levels significantly increased 15 years before the onset of dominantly inherited AD. The effects of high plasma levels of Aβ1−42 on mononuclear macrophage, the peripheral counterparts of microglia, remains unclear. Herein, we used a parabiosis model to investigate the effects of plasma Aβ1−42 on peripheral mononuclear macrophage and the possible mechanism. We found the proportions of pro-inflammatory macrophages in peritoneal cavity, myeloid derived suppressor cells (MDSCs) in spleen, granulocyte-monocyte progenitors (GMP) in bone marrow, and the plasma levels of interleukin-6 (IL-6) were significantly decreased after 4 months sustaining stimulation with high plasma levels of Aβ1−42. The proportions of pro-inflammatory macrophages, MDSCs, GMPs, the plasma levels of IL-6 and TNF-α, as well as the numbers of bone marrow-derived macrophages (BMDMs) in mice brain were increased after 8 months sustaining stimulation with high plasma levels of Aβ1−42. In addition, the proportions of monocytes in spleen and the proliferation of bone marrow cells (BMCs) were increased, however, the phagocytic function of macrophages had no obviously alteration after high plasma levels of Aβ1−42 sustaining stimulation. These results demonstrated that high plasma levels of Aβ1−42 had a biphasic regulating effects on the amounts of pro-inflammatory macrophages, MDSCs, and bone marrow myeloid progenitor cells, as well as the secretory function of macrophage. Herein, we suggest that the alterations of the amounts and proliferations of myeloid progenitor cells in bone marrow may be involved in the regulation of peripheral mononuclear macrophages and MDSCs, intervening the effects of plasma Aβ1−42 on monocytes/macrophages might offer a new therapeutic approach to AD.