bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulatory Role of Long Non-Coding RNA “Maternally-Expressed Gene-3” in Diabetic Nephropathy through Targeting microRNA-21 and Transforming Growth Factor-Beta
Authors: Elwi Heba Mostafa , El-Din Shimaa Saad , Rashed Laila , Sadek Noha Adly , Medhat Engy 
Published: March 2022

Abstract
Introduction: Diabetic nephropathy (DN) is a leading cause of chronic kidney disease and end-stage renal disease worldwide. The maternally expressed gene-3 (MEG3), a lncRNA, is implicated in the development of diabetic microvascular complications like retinopathy and nephropathy. MicroRNA-21 (miRNA-21) and Transforming Growth Factor-Beta (TGF-β) are established playmakers in fibrogenesis in DN. Research stated that MEG3 can sponge miRNA-21 inhibiting it. Additionally, MEG3 modulates the activity of TGF-β genes by binding to promotor-distal regulatory elements. Aim: Investigate MEG3 regulatory role in DN pathogenesis, and its possible inhibitory effect on miRNA-21 and TGF-β signaling pathway. Methods: The study was performed on 75 subjects divided into 3 groups: 25 controls, 25 diabetics, and 25 DN patients. Participants were subjected to routine laboratory investigations, estimation of gene expression of MEG3 and miRNA-21 by RT-qPCR and measurement of serum TGF-β levels by ELISA. Results: DN group shows a highly significant decrease in MEG-3 relative gene expression, and a significant increase in both miRNA-21 relative gene expression and TGF-β sera levels, when compared to diabetic and control groups. In addition, MEG3 is significantly negatively correlated to miRNA-21, TGF-β1, and HbA1c confirming the sponging effect of MEG3 on miRNA-21 and predicting its potential protective role in DN. On the other hand, there is a statistically significant positive correlation between miRNA-21 gene expression and TGF-β1 levels indicating the up-regulatory role of miRNA-21 on TGF-β1 protein expression levels. Conclusion: Our data suggests that MEG3 is implicated in DN pathogenesis through a possible loop encompassing miRNA-21 and TGF- β1.
The experiences and attitudes among Chinese soft tissue sarcoma patients: a qualitative study using expressive writing
Authors: Lin Nan , Deng Yaotiao , Wei Weige , Qin Taolin , Jiang Yu 
Published: March 2022

Abstract
PurposeWith poor diagnose and limited knowledge, soft tissue sarcoma (STS) patients were affected by their suffering. Chinese STS patients are often not willing to talk about their feelings and thoughts publicly, which may have negative impact on their psychological status. Expressive writing allows people to disclose their thoughts during writing, which has not yet been studied in patients with soft tissue sarcoma. This study was conducted to understand the experience and attitude of Chinese STS patients using expressive writing.MethodSeven participants with soft tissue sarcoma were included to take 6 episodes of expressive writing with structured guidance. Standard qualitative research method was used to analyze the writing materials.ResultFour themes were identified: treatment process, interpersonal relationship, coping strategies, views about the future. We depicted some crucial aspects of life for STS patients in China, that they may have many difficulties and struggles, but most of them still face life with a positive attitude.ConclusionExpressive writing was acceptable and feasible in Chinese STS patients. We revealed the dynamic process of acceptance and adaptation to life change in them. Itis necessary for doctors and nurses to understand the specific psychological status of STS patients in order to improve their quality of life.Trial registration: ChiCTR2000038923
Difference in leukemia microenvironment induced by PAX5 mutations in acute lymphoblastic leukemia
Authors: Huang Wei , Gong Duanhao , Wang Lei , Xu Fangfei 
Published: March 2022

Abstract
Background:PAX5 mutations are involved in the development and recurrence of leukemia. There were few studies on how PAX5 mutations influencing leukemia bone marrow microenvironment. Methods: We obtained the mRNA profiling of B-acute lymphoblastic leukemia(ALL) patients from GSE11877 and the clinical features (including molecular genetics) from the literature. We identified differentially expressed genes (DEGs) between B-ALL with PAX5 mutations and B-ALL without PAX5 mutations in leukemia microenvironment using the LIMMA package of R. We assessed immune cell types by CIBERSORT. DEGs were analyzed using the DAVID,STRING and Coexpedia. Results:The results from CIBERSORT analysis showed that M0 macrophages were significantly increased from ALL PAX5 mutations. The results from DAVID analysis indicated that these DEGs were mostly involved in phosphorylation and antigen presentation from biological process categories. These DEGs may be involved in changes in macrophages. Other analyses from PPI network and co -expression network found three common genes such as IL1B, CD34, PDGFRB, which were highly related to angiogenesis. Conclusions:These changes hinted that PAX5 mutations led to macrophage changes, and macrophage changes were related to processes of antigen presentation and angiogenesis in leukemia microenvironment.
Degradation Characteristics of Pinoxaden by Acinetobacter and Prediction of Related Genes
Authors: Yang Fengshan , Wei Yuning , Sun Cong , Yuan Mingrui , Zeng Weimin , Liu Chunguang , Fu Haiyan 
Published: March 2022

Abstract
In order to improve crop yield per unit area, pinoxaden was widely used in weed control in the field to better degrade the residual pinoxaden in the soil. In this experiment, 14 strains were domesticated and isolated from soil samples using pinoxaden as the only carbon and nitrogen source, and the highly degraded strain was T4P20-1. The effects of different pH values, inoculation amount and culture time on the growth and degradation rate of strain T4P20-1 were studied. The optimal growth conditions of strain T4P20-1 were determined, and the degradation rate reached 92.23%. The morphological characteristics of strain T4P20-1 were determined by optical microscope. The strain T4P20-1 was identified as Acinetobacter Pittii by physiological and biochemical characteristics and 16S rDNA homology analysis, and the nucleic acid sequence was submitted to Gentbank to obtain the accession number MW350066. Through gene sequencing, the whole genome framework of strain T4P20-1 and database annotations of GO, KEGG, COG, CAZymes and PHI were obtained. Meanwhile, genes related to herbicide degradation were predicted by gene functional annotations. There was a negative correlation between herbicide and seed germination rate, bud length and root length of maize susceptible to subsequent crop. In conclusion, the strains screened in this study have certain remediation effects on soil contaminated by pinoxaden. It provides a new choice for the degradation of pinoxaden in the future. It can increase crop yields and reduce food security risks.
Removal of mixed cationic dyes by a biosorbent based on magnetic tragacanth gum hydrogel
Authors: Jaymand Mehdi 
Published: March 2022

Abstract
A magnetic tragacanth gum-grafted poly(acrylic acid) hydrogel (TG-g-PAA/Fe3O4) was applied for removal of malachite green (MG), basic yellow 28 (BY28), and rhodamine 6G (Rh6G) cationic dyes form industrial simulated wastewater via the well-known batch method. The most important parameters such as initial dyes concentrations, adsorbent dosage, pH, and contact time that affected adsorption performance were optimized in all single, binary, and ternary systemes. The adsorption for all dyes in all systemes were well-fitted with Langmuir model than those of the Freundlich model that revealed the linearity of the process. Maximum adsorption capacities (Qm) for MG, BY28, and Rh6G in ternary system were obtained as 626.5, 568.2, and 459.7 mgg-1, resepectively. Kinetic studies exhibited that the removal of all dyes in all systems were best-fitted with the pseudo-second-order model, which proved that the rate-limiting step might be the chemical adsorption. The hydrogel was regenerated by desorption process after loading process and reused for several times. As results, the removal efficiency of adsorbent almost remains the same for the first four cycles, and then decreased gradually.
Comparative ability of EDTA, GLDA, and MGDA to desorb Pb from contaminated montmorillonite: Aging effects
Authors: Shirvani Mehran , Parsadoust Farzad , Shariatmadari Hossein , Dinari Mohammad 
Published: March 2022

Abstract
Study of Pb desorption processes from clay minerals by chelating ligands is crucial to better understand the fate and transport of Pb in soil and sediment environments. In this study, Pb desorption from a Pb-loaded montmorillonite (MMT) was studied as affected by ethylenediaminetetraacetic acid (EDTA) and its two alternative eco-friendly chelating agents, i.e., methylglycine diacetic acid (MGDA) and glutamic-N,N-diacetic acid (GLDA) at two concentrations of 0.25 and 1.0 mM. The impacts of a 30-day residence time on the rate and quantity of Pb desorption were also evaluated. The result showed that Pb desorption kinetics was biphasic with an initial rapid phase, lasting for about 3 h, followed by a slow phase, lasting to 12 h. The degree of Pb desorption from Ca-MMT was proven to be governed by the nature and concentration of the chelating ligands presented in the systems. The capacity of the ligands to desorb Pb from Ca-MMT was in the order EDTA > > MGDA > GLDA, according to the decreasing order in the stability constants of their complexes with Pb ions. The aging of the MMT systems caused a significant reduction in both Pb desorption quantity and rate parameters. The results suggest that EDTA would have a more serious Pb-mobilizing impact than GLDA and MGDA in contaminated soil and sediments which contain Ca-MMT as a major clay constituent.
Analysis of the intestinal flora of sucking goats with diarrhea by metagenomic Sequencing
Authors: Cheng Darong , Cheng Cheng , Zhu Shengmin , Chen Xiaofang , Tao Jianping 
Published: March 2022

Abstract
Background: Diarrhea is one of the important diseases of sucking goats and seriously affects the survival rate of lambs. Many studies have been reported on the etiology of goat diarrhea, but the available information on the intestinal flora disorder in diarrheic goats was limited. Methods: To understand the impact of the gut microbes on goat health and to determine the changes in the intestinal flora of sucking goats with diarrhea, 13 fecal samples from 9 diarrheic lambs and 4 healthy lambs were collected and submitted to metagenomics analysis. Results: The data show that the intestinal flora of diarrheic goats was not only significant changed in the bacterial species, but also displayed abundances decrease in probiotics, such as Butyricicoccus pullicaecorum, Ruminococcus gnavus, Bacteroides fragilis, etc. Many harmful bacteria harboring in the intestinal flora and pathogenic bacteria invading from in vitro should be important bacteria that cause intestinal flora disorder. The most dominant harmful bacteria include Staphylococcus aureus, Lactococcus garvieae, Helicobacter pylori, Escherichia coli, Firmicutes bacterium, Bacteroides plebeius, etc. Conclusion: The intestine flora of sucking goats with diarrhea changed significantly in both species and abundance. Disorder of intestinal flora should be one of the important causes of diarrhea in sucking goats.
Psychiatric emergencies during, after, and before the COVID-19 lockdown; what happened to our patients? A naturalistic observational study
Authors: Brandizzi Martina , Polselli Annalivia , Corigliano Valentina , Tamorri Stefano Maria , Venturini Paola , Azzoni Antonella , Grasso Silvia , Onofri Antonio , Pesce Salvatore , Romani Fiammetta , Polselli Gian Marco , Forte Alberto 
Published: March 2022

Abstract
Background: Despite concerns on mental health problems related to COVID19 lockdown, recent reports revealed a reduction in psychiatric admissions in Emergency Departments (ED) during the lockdown period compared with the previous year in several countries. Most of the existing studies focused on the first lockdown not considering the different phases of the COVID-19 crisis. The present study aimed to analyze differences in ED admission for psychiatric consultation during three different phases of the COVID-19 pandemia in Italy. Methods: Information on ED admission for psychiatric consultations were retrospectively collected at the ED of the Santo Spirito Hospital in Rome (Italy), and compared between the three periods: the lockdown (March - June 2020) and the post-lockdown period (June 2020 - June 2021) compared to the pre-lockdown (January 2019 - March 2020). Multinomial logistic regression was used to assess the risk of accessing ED for psychiatric consultation before, during, after the lockdown. Results: 3,871 ED psychiatric consultations were collected. A significant reduction of psychiatric consultations in ED during the lockdown period and the post-lockdown (H: 762,45; p<0.001) was documented. Multinomial logistic regression analysis showed that compared to pre-lockdown during the lockdown and post-lockdown patients were more likely to be men (RRR: 1.52; 95% CI: 1.10 - 2.12) and more often diagnosed with non-severe mental illnesses (nSMI) (relative risk ratio [RRR]: 1.53, 95% CI: 1.10 - 2.15; and 1.72, 95% CI: 1.42 - 2.08); during the lockdown, patients were also more often diagnosed with alcohol/substance abuse (A&S) (RRR: 1.70; 95% CI: 1.10 - 2.65). Conclusions: Several changes in the clinical characteristics of psychiatric consultations during and after the lockdown emerged from the present study; nSMI and A&S abuse patients were more likely to present at the ED in the lockdown and post-lockdown periods while SMI patients appeared to be less likely. These may inform clinicians and future preventive strategies among community mental health services.
Uveitis Including Vogt-Koyanagi-Harada Syndrome Following Inactive Covid-19 Vaccination: A Case Series
Authors: Shariati Mehrdad Motamed , Abrishami Mojtaba , Jahani Shahin , Bolouki Ali , Astaneh Mohamadreza Ansari , Hosseini Seyedeh Maryam 
Published: March 2022

Abstract
Background: Currently, large populations around the world have been vaccinated against COVID-19. The whole inactivated Sinopharm COVID-19 vaccine has been the main available and widely used COVID-19 vaccine in Iran. Ocular inflammatory reactions have been reported following vaccination. This case report aimed to introduce four cases of uveitis after Sinopharm vaccine inoculation. Case presentation: The first case was a 38-year-old woman who was a known case of ulcerative colitis whose systemic disease was inactive during the past four years. Active uveitis was developed following the second dose of the COVID-19 vaccination. The other three cases were completely healthy individuals who developed the first episode of uveitis, including one case of Vogt-Koyanagi-Harada syndrome, after the COVID-19 vaccination. All cases responded well to corticosteroid treatments.Conclusion: our cases and ongoing reports around the world raise concerns about the possibility of uveal inflammations in cases with a history of auto-immune systemic diseases or inactive uveitis after the COVID-19 vaccination.
Impact of untethering of the spinal cord on urological management and antibiotic stewardship among spina bifida patients in Malaysia
Authors: Nisheljeet Singh , Azizi Abu Bakar , Palaniandy Kamalanathan , Ganesan Dharmendra , Ong Teng-Aik , Alias Azmi , Rajamanickam Ramalinggam , Atroosh Wahib Mohammed , Mohd-Zin Siti Waheeda , Lee-Shamsuddin Andrea , Nivrenjeet Singh , Abdul-Aziz Noraishah Mydin 
Published: March 2022

Abstract
BackgroundThe incidence and severity of urinary tract infections (UTIs) due to spina bifida is poorly understood in Malaysia. Tethering of the spinal cord is a pathological fixation of the cord in the vertebral column that can result in neurogenic bladder dysfunction and other neurological problems. It occurs in patients with spina bifida and the authors of this study sought to determine the effects of untethering on the urological outcome of children with a tethered cord and thus identifying proper urological management for individuals with spina bifida. MethodsDemographic and clinical data were collected via an online questionnaire and convenient sampling technique were used. 49 spina bifida individuals participated in this study. ResultsThe diagnosis of UTIs is dependent on patients reporting cloudy and smelly urine (67%) and urine validation (60%). 18% of the respondents claimed to have been prescribed antibiotics even though they had no history of UTI. Indiscriminate prescription of antibiotics by healthcare workers further compounds the severity of UTI. Employing CISC (73%) including stringent usage of sterile catheters (71%) did not prevent patients from getting UTI. Overall, 33% of our respondents reported manageable control of UTI (0 – 35 years of age). All individuals below the age of 5 (n=14) had improved urological outcome due to the untethering surgery under the guidance of the Malaysia NTD support group. ConclusionSpina biﬁda individuals may procure healthy bladder and bowel continence for the rest of their lives provided that neurosurgical and urological treatment being instigated soon after birth and continuing into adulthood.
Successful multidisciplinary treatment with laparoscopic hepatectomy and adjuvant therapy for metachronous solitary hepatic metastasis after excision of a primary anorectal malignant melanoma: A case report
Authors: Yokoo Hideki , Shimazaki Ryotaro , Hagiwara Masahiro , Tani Chikayoshi , Iwata Hiroyoshi , Takahashi Hiroyuki , Imai Koji , Fukuyama Marika , Matsuya Taisuke , Yamamoto Akemi , Yuzawa Sayaka , Tanino Mishie , Matsuno Naoto 
Published: March 2022

Abstract
Background: Anorectal malignant melanoma (ARMM) is extremely rare and generally lethal, irrespective of the treatment administered. The disease is often diagnosed late, metastases being present in approximately two-third of patients at the time of initial diagnosis. Solitary metastasis of ARMM to a distant organ is exceedingly rare. To the best of our knowledge, only two cases of hepatic resection for a synchronous solitary liver metastasis from a primary ARMM have been reported, and only one of hepatic resection for a metachronous solitary liver metastasis after excision of a primary ARMM. Additionally, we did not find any previous reports of multidisciplinary therapy incorporating surgery and a full course of adjuvant therapy for the latter condition. Case presentation: A 76-year-old woman with a history of laparoscopic abdominoperineal resection of an ARMM 13 months previously was found to have a solitary liver metastasis on follow-up computed tomography. A preoperative work-up showed no other distant metastases nor contraindication to surgery. It was therefore considered that resection was indicated. The metachronous solitary liver metastasis from an ARMM was treated by laparoscopic wedge hepatectomy of the eighth segment 18 months after excision of her primary ARMM. Adjuvant therapy with pembrolizumab was initiated and continued at 6-week intervals. The patient has now completed 12 months of adjuvant pembrolizumab therapy, having survived 33 months from the initial operation for primary ARMM, and remaining recurrence-free 14 months after hepatectomy.Conclusions: We here report a rare case of hepatectomy for a solitary metachronous liver metastasis after resection of a primary ARMM, followed by completion of 12 months of adjuvant therapy with pembrolizumab. ARMM is extremely rare and resection of a metachronous solitary metastasis followed by adjuvant therapy has not previously been reported. We hope this case will be useful for clinicians treating similar patients.
The design process of the chassis of a prototype vehicle for Shell Eco-marathon
Authors: Odi-Owei Steven , Anisiji Emeka Obiora , Erukainure Frank Efe 
Published: March 2022

Abstract
This paper presents the design, modelling, and fabrication procedures for the chassis of a prototype car with the aim of achieving the objectives: rigid and high strength chassis, reduced vehicle weight, driver safety, and an energy efficient vehicle. The novelty of this work is that it demonstrates how aluminium alloy could be used to construct non-integrated chassis for super mileage vehicles through a load-stress calculation model. Furthermore, a method of Finite Element Analysis (FEA) was presented which achieves the same result as the analytical calculation. The work also presents a method of fabricating aluminium square tube for the chassis by joining two angle bars. The results show that the FEA approach agrees with the analytical design model thereby reducing the time consumed in conceptual design process. The fabricated chassis was found to show no cracks and it was able to resist bending and shear from the loads acting on the vehicle in line with the design data. The energy efficiency recorded from the first test-run of the vehicle was 250 km/L of gasoline. The methods presented could be characterized as accurate and reliable for manufacturers of super mileage racing cars for Shell Eco-marathon events.
Feasibility indicators in obesity-related behavioral intervention preliminary studies: A historical scoping review
Authors: Pfledderer Christopher D , von Klinggraeff Lauren , Burkart Sarah , Wolfenden Luke , Ioannidis John P.A. , Beets Michael W. 
Published: March 2022

Abstract
Background: Behavioral interventions are often complex, operate at multiple levels, across settings, and employ a range of behavior change techniques. Collecting and reporting key indicators of initial trial and intervention feasibility is essential to decisions for progressing to larger-scale trials. The extent of reporting on feasibility indicators and how this may have changed over time is unknown. The aims of this study were to: 1) conduct a historical scoping review of the reporting of feasibility indicators in behavioral pilot/feasibility studies related to obesity published through 2020, and 2) describe trends in the amount and type of feasibility indicators reported in studies published across three time periods: 1982-2006, 2011-2013, and 2018-2020.Methods: A search of online databases (PubMed, Embase, EBSCOhost, Web of Science) for health behavior pilot/feasibility studies related to obesity published up to 12/31/2020 was conducted and a random sample of 600 studies, 200 from each of the three timepoints (1982-2006, 2011-2013, and 2018-2020) was included in this review. The presence/absence of feasibility indicators, including recruitment, retention, participant acceptability, attendance, compliance, and fidelity, were identified/coded for each study. Univariate logistic regression models were employed to assess changes in the reporting of feasibility indicators across time.Results: A total of 16,365 unique articles were identified of which 6,873 of these were reviewed to arrive at the final sample of 600 studies. For the total sample, 428 (71.3%) studies provided recruitment information, 595 (99.2%) provided retention information, 219 (36.5%) reported quantitative acceptability outcomes, 157 (26.2%) reported qualitative acceptability outcomes, 199 (33.2%) reported attendance, 187 (31.2%) reported participant compliance, 23 (3.8%) reported cost information, and 85 (14.2%) reported treatment fidelity outcomes. When compared to the Early Group (1982-2006), studies in the Late Group (2018-2020) were more likely to report recruitment information (OR=1.60, 95%CI:1.03-2.49), acceptability-related quantitative (OR=2.68, 95%CI:1.76-4.08) and qualitative (OR=2.32, 95%CI:1.48-3.65) outcomes, compliance outcomes (OR=2.29, 95%CI:1.49-3.52), and fidelity outcomes (OR=2.13, 95%CI:1.21, 3.77).Conclusion: The reporting of feasibility indicators within behavioral pilot/feasibility studies has improved across time, but key aspects of feasibility, such as fidelity, are still not reported in the majority of studies. Given the importance of behavioral intervention pilot/feasibility studies in the translational science spectrum, there is a need for improving the reporting of feasibility indicators.