bluuebunny/crossref_metadata_2025_split huggingface dataset:
The theranostic potentialities of bioavailable nanocurcumin in oral cancer management
Authors: Essawy Marwa M. , Mohamed Mostafa M. , Raslan Hanaa S. , Rafik Salma T. , Awaad Ashraf K. , Ramadan Omneya R. 
Published: November 2022

Abstract
Background
Oral cancer, one of the most common cancers, has unimproved 5-years survival rate in the last 30 years and the chemo/radiotherapy-associated morbidity. Therefore, intervention strategies that evade harmful side effects of the conventional treatment modalities are of need. Herbal therapy as a complementary preventive/therapeutic modality has gained attention. Curcumin is one of the herbal compounds possessing unique anticancer activity and luminescent optical properties. However, its low water solubility limits its efficacy. In contrast, curcumin at the nanoscale shows altered physical properties with enhancing bioavailability.

Methods
The current study evaluated the impact of nanocurcumin as an anti-oral cancer herbal remedy, comparing its efficacy against the native curcumin complement and conventional chemotherapeutic. An optimized polymeric-stabilized nanocurcumin was synthesized using the solvent-antisolvent precipitation technique. After assuring the solubility and biocompatibility of nanocurcumin, we determined its cytotoxic dose in treating the squamous cell carcinoma cell line. We then evaluated the anti-tumorigenic activity of the nano-herb in inhibiting wound closure and the cytological alterations of the treated cancer cells. Furthermore, the cellular uptake of the nanocurcumin was assessed depending on its autofluorescence.

Results
The hydrophilic optimized nanocurcumin has a potent cancerous cytotoxicity at a lower dose (60.8 µg/mL) than the native curcumin particles (212.4 µg/mL) that precipitated on high doses hindering their cellular uptake. Moreover, the nanocurcumin showed differential targeting of the cancer cells over the normal fibroblasts with a selectivity index of 4.5. With the confocal microscopy, the luminescent nanoparticles showed gradual nuclear and cytoplasmic uptake with apparent apoptotic cell death, over the fluorescent doxorubicin with its necrotic effect. Furthermore, the nanocurcumin superiorly inhibited the migration of cancer cells by -25%.

Conclusions
The bioavailable nanocurcumin has better apoptotic cytotoxicity. Moreover, its superior luminescence promotes the theranostic potentialities of the nano-herb combating oral cancer.
How should healthcare be reported in Catalonia? Qualitative study with healthcare leaders
Authors: García-Altés Anna , Aguado Hortensia , Guilabert Mercedes , Carrillo Irene , Mira Jose Joaquín 
Published: November 2022

Abstract
Background
The Results Centre is the name of a project that, since 2012, has been openly publishing the results of each healthcare centre in Catalonia, with the idea of promoting benchmarking among centres and transparency toward society. As the project evolves, it has become increasingly necessary to adapt its contents and formats. The objective of this study is to identify the preferences and expectations of healthcare leaders regarding the Results Centre.

Methods
A qualitative study was conducted using the nominal group technique. Five nominal groups were created with the participation of 58 professionals (26 from hospital care, 16 from primary care, and 16 from long-term care centres). The areas of analysis were: (1) what the Results Centre of the future should be like; (2) what information needs should be addressed; and (3) what novelties should be incorporated to stimulate quality improvement. The spontaneity of ideas, intensity of recommendations, and intergroup consistency were analysed. The study was conducted in April 2019.

Results
The requirements reported by the participants to be met by the Results Centre included: being a tool for benchmarking and strategic decision-making; adjusted and segmented indicators; non-clinical information (patient experience, socio-economic status, etc.); and data accessible to all stakeholders, including citizens. The ideas were consistent across the different levels of care, although the intensity of recommendations varied depending on their content.

Conclusions
Regional agencies that are accountable for health outcomes should be consistently committed to adapting to the needs of different stakeholders in the health system. This project is an example of how this requirement has been addressed in Catalonia.
Development and validation of a novel Spectrofluorimetric method of oral anticoagulant Edoxaban via derivatization with 9-fluorenyl methyl chloroformate: green assessment of the method by Eco-Scale and ComplexGAPI
Authors: Rizk Mohamed , El-Alamin Maha Mahmoud Abou , Elkhalek Ola Abd , Shallan Aliaa I. 
Published: November 2022

AbstractA precise, sensitive eco-friendly, simple, rapid, and derivative spectrofluorimetric method was developed to quantify edoxaban tosylate monohydrate in pure form and pharmaceutical dosage form. Sudden death due to pulmonary embolism as a consequence of coronavirus infection (covid-19) is an emerging problem. As a result, the world health organization introduced new guidelines to treat patients with COVID-19 with oral anticoagulants. Edoxaban tosylate monohydrate is an oral anticoagulant that doesn’t require hospitalization after dose adjustment. This spectrofluorimetric method relies on the derivatization by 9-fluorenyl methyl chloroformate at room temperature in borate buffer pH 9.0. After excitation at 265 nm, the product is highly fluorescent at 309 nm. Many experimental factors influencing the reaction's stability and development were thoroughly investigated and optimized. The method validation was evaluated by using ICH guidelines and showed high precision and accuracy with an average percent recovery of 101.46% ± 1.02. The linear range was 5.0–50.0 ng/mL with a correlation coefficient of 0.9999, the LOD was 1.5 ng/mL, and the LOQ was 4.5 ng/mL. The green assessment of the method was achieved utilizing the eco-scale and the Green Analytical Procedure Index. There was no significant difference between the results of the suggested method and those of the reported method according to Statistical analysis.
A multi-dimensional extensible cloud-native service stack for enterprises
Authors: Lin Jian , Xie Dongming , Huang Jinjun , Liao Zinan , Ye Long 
Published: November 2022

AbstractWith the widespread acceptance of the cloud-native concept and the emergence of a large number of dedicated cloud-native applications, the service stacks of cloud-native applications have received extensive attention in the industry. To analyze the extensibility problems of service stacks, a cloud-native light-cone model is proposed, which focuses on the dimensions of application, infrastructure, tenant and workflow, and provides a perspective view that reflects the concerns of stakeholders. Based on this model, various challenges in designing extensible cloud-native service stacks are identified by classification. To solve these challenges, a holistic architecture and a set of key technologies are designed, involving unified runtime abstraction, cluster bootstrapped creation, application-specific controllers, etc. Furthermore, the OMStack (Oriental Mind Stack) is implemented, which integrates these technologies and provides a group of PaaS and SaaS services for container cluster (OMCC), artificial intelligence (OMAI), big data (OMBD) and so on. Experimental analysis and production applications demonstrate the practicality, efficiency and reliability of the proposed architecture, stack and services.
Whole-exome sequencing of BRCA-negative breast cancer patients and case–control analyses identify variants associated with breast cancer susceptibility
Authors: Lee Ning Yuan , Hum Melissa , Amali Aseervatham Anusha , Lim Wei Kiat , Wong Matthew , Myint Matthew Khine , Tay Ru Jin , Ong Pei-Yi , Samol Jens , Lim Chia Wei , Ang Peter , Tan Min-Han , Lee Soo-Chin , Lee Ann S. G. 
Published: November 2022

AbstractBackgroundFor the majority of individuals with early-onset or familial breast cancer referred for genetic testing, the genetic basis of their familial breast cancer remains unexplained. To identify novel germline variants associated with breast cancer predisposition, whole-exome sequencing (WES) was performed.MethodsWES on 290BRCA1/BRCA2-negative Singaporeans with early-onset breast cancer and/or a family history of breast cancer was done. Case–control analysis against the East-Asian subpopulation (EAS) from the Genome Aggregation Database (gnomAD) identified variants enriched in cases, which were further selected by occurrence in cancer gene databases. Variants were further evaluated in repeated case–control analyses using a second case cohort from the database of Genotypes and Phenotypes (dbGaP) comprising 466 early-onset breast cancer patients from the United States, and a Singapore SG10K_Health control cohort.ResultsForty-nine breast cancer-associated germline pathogenic variants in 37 genes were identified in Singapore cases versus gnomAD (EAS). Compared against SG10K_Health controls, 13 of 49 variants remain significantly enriched (False Discovery Rate (FDR)-adjustedp < 0.05). Comparing these 49 variants in dbGaP cases against gnomAD (EAS) and SG10K_Health controls revealed 23 concordant variants that were significantly enriched (FDR-adjustedp < 0.05). Fourteen variants were consistently enriched in breast cancer cases across all comparisons (FDR-adjustedp < 0.05). Seven variants inGPRIN2, NRG1, MYO5A, CLIP1,CUX1, GNASandMGAwere confirmed by Sanger sequencing.ConclusionsIn conclusion, we have identified pathogenic variants in genes associated with breast cancer predisposition. Importantly, many of these variants were significant in a second case cohort from dbGaP, suggesting that the strategy of using case–control analysis to select variants could potentially be utilized for identifying variants associated with cancer susceptibility.
Clean recovery and recycling of seasonal surplus forage grass by microbial driven anaerobic fermentation: a case study of napiergrass
Authors: Zhao Jie , Yin Xue-Jing , Li Jun-Feng , Wang Si-Ran , Dong Zhi-Hao , Shao Tao 
Published: November 2022

AbstractIn this study, the anaerobic fermentation technique was conducted to accomplish the clean recycling of surplus napiergrass. The surplus napiergrass harvested at two harvest dates (early vegetative stage, NI; late vegetative stage, NII) was treated as follows: (i) natural fermentation of NI (NNI); (ii) natural fermentation of NII (NNII) and stored for 1, 3, 7, 15, 30 and 60 days. After 60 days of anaerobic fermentation, NNI had higher lactic acid concentration and ratio of lactic to acetic acid, but lower pH value and ammonia–nitrogen concentration than NNII. Lactobacillus and Enterobacter were, respectively, dominant in both 7-day NNI and NNII, while Lactobacillus was the most abundant genus in 30-day NNI and NNII. Both harvest date and store time altered the bacterial co-occurrence networks of fresh and fermented napiergrass. The complexity of the bacterial networks decreased from NII, NI, NNII to NNI. The correlations were primarily positive in the bacterial networks of NI, NII, NNII-7 and NNII-30 with positive correlative proportion of 53.0%, 64.3%, 53.1% and 55.6%, but negative in those of NNI-7 (47.4%) and NNI-30 (46.2%) with positive correlative proportion of 47.4% and 46.2%, respectively. Overall, the fermentation quality and microbial community structure of napiergrass during anaerobic fermentation were highly influenced by harvest date and store time. Based on the principle of stable fermentation and high quality, anaerobic fermentation of NI for at least 15 days is recommended. The in-depth understanding of microbial community dynamics and co-occurrence networks during anaerobic fermentation of napiergrass is important for revealing the fermentation mechanism and can contribute to resource recycling without increasing cost.
Graphical Abstract
Individual and contextual factors associated with childhood malnutrition: a multilevel analysis of the double burden of childhood malnutrition in 27 countries
Authors: Ijaiya Mukhtar A. , Anjorin Seun , Uthman Olalekan A. 
Published: November 2022

Abstract
Background
Malnutrition is a key global health challenge and a major contributor to childhood morbidity and mortality. In recent times, the contrasting coexistence of undernutrition including micronutrient deficiencies and overweight/obesity called double burden of malnutrition has been noted at individual, household or population level and/or at different times in life. The objective of this study was to examine individual, neighborhood and country level factors that are associated with the double burden of childhood malnutrition.

Methods
We conducted multivariable multilevel logistic regression analyses on the most recent demographic and health datasets from surveys conducted between 2015 and 2020 in low- and middle-income countries. We analyzed data of 138,782 children (level 1) living in 13,788 communities (level 2) from 27 countries (level 3).

Results
The results of our analysis show variation in childhood malnutrition across the 27 countries from as low as 6.5% in Burundi to as high as 29.5% in Timor Leste. After adjusting for all level factors, we found that those who were wasted/overweight tended to have had an episode of diarrhea or fever in the last two weeks preceding the survey, were part of a multiple birth, were being breastfed at the time of the survey and born to mothers with more than one under 5-child resident in neighborhoods with high illiteracy and unemployment rates. The intra-neighbourhood and intra-country correlation coefficients were estimated using the intercept component variance; 44.3% and 21.0% of variance in odds of double burden of childhood malnutrition are consequent upon neighborhood and country level factors respectively.

Conclusions
Evidence of geographical clustering in childhood malnutrition at community and country levels was found in our study with variability due to neighborhood level factors twice that of country level factors. Therefore, strategies in tackling the double burden of malnutrition must consider these shared drivers, contextual barriers and geographical clustering effects.
Diagnostic performance of ADC value in histopathological grading of mass-forming cholangiocarcinoma
Authors: Ellaban Heba Said , El-Kholy Mohamed R. , Abdellah Tarek F. , Abdelgawad Mohamed Saied 
Published: November 2022

Abstract
Background
Various invasive and non-invasive imaging modalities are used to diagnose and stage cholangiocarcinoma (CC). In this study, we aimed to assess the role of diffusion-weighted imaging (DWI) and apparent diffusion coefficient (ADC) value measurement in diagnosing and grading CC with mass formation. The study was conducted on 38 cases, including 24 males and 14 females, with ages ranging from 34 to 77 years with a mean age of 61.52 ± 10.45 year. Patients were referred by the Hepatobiliary Surgery and Hepatology departments of our institution between October 2019 and November 2021. With respect to the patients diagnosed with mass-forming CC by pathology, they underwent dynamic MR 1.5 T. CC was evaluated qualitatively using visual analysis of DW-MR images and quantitatively with measurement of ADC values.

Results
The mean ADC of mass-forming carcinoma was lower than that of hepatic parenchyma and more than that of the splenic parenchyma. Poorly differentiated CC was associated with a lower ADC value (mean 0.98 ± 0.12), while well-differentiated CC was associated with a higher ADC value (mean 1.23 ± 0.16). The cutoff value for poorly differentiated CC ADC measurements was less than 1.15, whereas it was greater than 1.15 for the well-differentiated CC.

Conclusions
DWI is a new effective technique for detecting and improving CC diagnosis as a safe, non-invasive, and non-contrast dependent.
Factors influencing COVID-19 vaccine acceptance and hesitancy among pharmacy students in Bangladesh: a cross-sectional study
Authors: Roy Debendra Nath , Hossen Md. Mohabbot , Biswas Mohitosh , Islam Ekramul , Azam Md. Shah 
Published: November 2022

Background: In the wake of COVID-19 prevention, a growing attention has been devoted to administering vaccines among various sub-group populations including community health care providers. As a community health worker pharmacists and pharmacy students played a crucial role in patient-centered services for managing COVID-19. Examining pharmacy students’ vaccine acceptance intent has great potential in understanding how pharmacists’ perception impacts community people. This study investigated COVID-19 vaccine acceptance among pharmacy students in Bangladesh and identified the potential factors associated with their vaccine acceptance and hesitancy. Methods: An anonymous questionnaire was deployed online using Google forms in English and conveniently sent to 1190 pharmacy students at different universities between 15th October 2021 and 15th December 2021. The convenience sampling consisted of 1034 student pharmacists (response rate 86.9%) who participated in this study. Binary logistic regressions and Chi-squared test were used for rationalizing the study objectives. Results: The pooled COVID-19 vaccine acceptance rate was 908 (87.8%; 95% CI 85.8─87.8) among student pharmacists while 29.6% (95% CI 25.4─33.9) admitted the willingness to pay (WTP) for a COVID-19 vaccine. Out of 12 vital predictive factors, “safety,” “efficacy,” and “trust” had the strongest significant and positive association with vaccine acceptance (p=0.000). The logistic analysis also revealed that “communication” and “information sufficiency” had a significant positive association with vaccine acceptance (p=0.035 and 0.038, respectively) among student pharmacists. Although the odds of accepting the COVID-19 vaccine were found to be 1.1; an insignificant association between gender and vaccine acceptance was observed in the Chi-squared test. Conclusions: The prevalence of COVID-19 vaccine apprehension will reduce if vaccine-related information becomes more publicly available. Ensuring easy access to scientific information with evidence-based and tailored communication would enhance vaccine acceptance among pharmacy students. Implementation of multidisciplinary educational intervention would support the health care students to achieve adequate knowledge on vaccination consequences.
Case Report: An unusual case of small bowel volvulus due to appendicitis associated with pneumatosis intestinalis: Review of the literature
Authors: Zouaghi Alia , Hadded Dhafer , Meryam Mesbahi , Benzarti Yazid , Cherif Mona , Zaafouri Haithem , Ben Massoud Khalil , Chamekhi Chiraz , Ben Maamer Anis 
Published: November 2022

Pneumatosis cystoid intestinalis is a rare disease reported in the literature affecting 0.03% of the population. It has a variety of causes and its manifestation may change widely. It usually presents as a marginal finding resulting from various gastrointestinal pathologies. In the acute complicated form of pneumatosis intestinalis, management is challenging for physicians and surgeons. We present a case of a 60-year-old patient who was admitted to our surgical department with a symptomatology suggestive of small bowel occlusion. Computed tomography demonstrated ileal volvulus associated with parietal signs suffering and pneumoperitoneum. An emergent exploratory laparoscopy followed by conversion was performed demonstrating segmental ileal pneumatosis intestinalis secondary to a small bowel volvulus due to an inflammatory appendix wrapping around the distal ileum. Further, detorsion, retrograde draining, and appendectomy were performed because there were no signs of necrosis and the appendix was pathological. The postoperative course was uneventful. This case is exceedingly rare in the literature, because it was featured by the ileal volvulus due to appendicitis.This case report emphasizes the importance of surgical procedures in the management of symptomatic pneumatosis intestinalis associated to an acute abdomen.
Avon Community-Acquired Pneumonia (AvonCAP) Surveillance Study: A Pan-pandemic Acute Lower Respiratory Tract Disease Surveillance Study, and the linked records of the participants in the Avon Longitudinal Study of Parents and Children (ALSPAC)
Authors: Mumme Mark , Clout Madeleine , Grimes Charli , Calkin Rachel , Finn Adam , Hyams Catherine , Macleod John 
Published: November 2022

This data note describes the linking of records of the Avon Community-Acquired Pneumonia (AvonCAP) Surveillance Study – A Pan-pandemic Acute Lower Respiratory Tract Disease Surveillance Study (ISRCTN: 17354061), with the participants of the Avon Longitudinal Study of Parents and Children (ALSPAC – also known as ‘Children of the 90s’). These records were obtained from a database created specifically by AvonCAP operating within the two acute care NHS Trusts in Bristol, which is the centre of the ALSPAC study catchment area. These two trusts are the (a) North Bristol NHS Trust (NBT) based at Southmead Hospital (SMH) and (b) the University Hospitals Bristol and Weston NHS Foundation Trust (UHBWT) based at Bristol Royal Infirmary (BRI). The AvonCAP database was updated by staff using a combination of clinical records and self-reporting by the participant, which was unable to be independently verified. Data was collected using the REDCap (Research Electronic Data Capture) software program. Software code was created to transform the original files into a single data base which was reviewed for data-completeness and for its potential value as a research resource. The AvonCAP records provide a contemporaneous record of a subset of the ALSPAC cohort who experienced Lower Respiratory Tract Disease. It is available for research and may be used in conjunction with other ALSPAC data.
Spatio-temporal occurrence, burden, risk factors and modelling methods for estimating scrub typhus burden from global to subnational resolutions: a systematic review protocol
Authors: Wang Qian , Sartorius Benn , Philip John Day Nicholas , James Maude Richard 
Published: November 2022

Background: Scrub typhus is a neglected life-threatening vector-borne disease mainly caused by the bacterium Orientia tsutsugamushi, which is occasionally transmitted to humans during feeding of larval mites. It has been estimated that more than 1 billion persons potentially threatened and 1 million clinical cases occur annually across the world; however, it is unclear how this estimate was computed (and what the original source was) and much remains unknown regarding its global burden and risk factors. This systematic review aims to provide a comprehensive overview of the spatial-temporal distribution of scrub typhus, associated burden and risk factors at global, national and subnational resolutions, and to review the burden estimation models used at those different scales. Methods: A systematic search for literature on scrub typhus occurrence, risk factors and modelling methods will be conducted. PubMed and five other databases will be searched for published literature, and Google Scholar and nine other databases will be used to search for grey literatures. All titles/abstracts of the searched records will be separately assessed by two reviewers, who will then screen the full-text of potential records to decide eligibility. Two reviewers will independently perform corresponding data extraction and finally cross-check using designed standardized forms. Data will be tabulated, synthesized descriptively, and summarized narratively for each review question. Where appropriate, meta-analyses will be conducted. The risk of bias will be assessed, and potential publication bias will be detected. Discussion: This review will provide a comprehensive understanding of the current occurrence, spatial-temporal distribution, and burden of scrub typhus, identify associated risk factors from global to subnational resolutions, consolidate the best practice modeling framework(s) to estimate the burden of scrub typhus at various geographic/temporal resolutions, and decompose the relative contributions of various risk factors at scale. PROSPERO Registration: CRD42022315209