bluuebunny/crossref_metadata_2025_split huggingface dataset:
The compact genome of a new Caenorhabditis species Caenorhabditis niphades n. sp., isolated from Niphades beetles
Authors: Sun Simo , Kanzaki Natsumi , Dayi Mehmet , Maeda Yasunobu , Yoshida Akemi , Tanaka Ryusei , Kikuchi Taisei 
Published: September 2022

Abstract
The first metazoan genome sequenced, that of Caenorhabditis elegans, impulse animal genome evolution studies. To date > 50 species from the genus Caenorhabditis have been sequenced, allowing research on genome variation. In the present study, we describe a new gonochoristic species, Caenorhabditis niphades n. sp., previously referred as C. sp. 36, isolated from adult weevils (Niphades variegatus), with whom they appear to be tightly associated during its life cycle. Along with a species description, we sequenced the genome of C. niphades n. sp. and produced a chromosome-level assembly. A genome comparison highlighted that C. niphades n. sp. has the smallest genome (59 Mbp) so far sequenced in the Elegans supergroup, despite of being closely related with the largest genome species, C. japonica. The compact genome of C. niphades n. sp. can serve as a key resource for comparative evolutionary studies of genome and gene number expansions in Caenorhabditis species.
The roles of estrogen receptor alpha (ERα) in endometrial carcinoma
Authors: Ge Yidong , Ni Xiaoqi , Li Jingyun , Ye Meng , Jin xiaofeng 
Published: September 2022

Abstract
Endometrial carcinoma (EC) is a group of endometrial epithelial malignancies, most of which are adenocarcinomas and occur in perimenopausal and postmenopausal women. It is one of the most common carcinomas of the female reproductive system, with a mortality rate only after to ovarian and cervical cancer. Existing studies have shown that the occurrence and development of EC is closely related to estrogen (E2) and estrogen receptor, especially estrogen receptor alpha (ERα). ERα, as a key nuclear transcriptional factor, is mainly an oncogenic factor in EC. Its interaction with upstream, co-regulators and downstream is important in the proliferation, metastasis, invasion and anti-apoptosis of EC. In this review, the structure of ERα and the regulation of ERα in multiple dimensions are described. In addition, the classical E2/ERα signaling pathway and the crosstalk between ERα and other EC regulators are elucidated, as well as a therapeutic target of ERα, which may provide a new direction for clinical applications of ERα in the future.
Advertising Contents of Child Health Drinks in India
Authors: Dey Sri Rajib , Barman Arup , Chakraborty Nilanjana , Elngar Ahmed A. 
Published: September 2022

Abstract
The objective of this paper is to study the trend and pattern of advertising content for child health drinks in India. A detailed analysis of the advertisement content of 5 child health drink brands for 6 years has been undertaken in this study. The majority of the brand's core focus area was growth. The most highlighted nutrient among the brand was protein. Maximum brands have highlighted more than one nutrient in their advertisements. Most of the brands have displayed scientific information and 100% of the brands have shown health benefits in their ads. The celebrity or HCP was present in most of the brand’s advertisements. Regarding location, it was found that in the majority of the brand's advertisements there was a combination of location i.e., home, leisure & shop. All the brands have used rational and emotional appeals in their advertisements. In the majority of the brands' advertisements, the lead voice was female. In child-parent interaction, most of the brands have shown the mother in the parents’ position. However, in the advertisement content, there was no verbal mention of a balanced diet, RDA, and calories which are very important from a child’s health point of view. As there are several studies that suggest the rise in childhood obesity and the association of television advertisements with it. The policymakers also need to closely look into the advertising content and act for the benefit of the child’s health.
An Investigation of the Maintenance Mechanisms of the Quasi- biweekly Pacific-Japan Teleconnection
Authors: Zhu Yu , Chen Ruidan , Song Qingtao , Li Xiuzhen , Guo Yuanyuan , Wen Zhiping 
Published: September 2022

Abstract
The intraseasonal Pacific–Japan (PJ) pattern, characterized by a pronounced quasi-biweekly oscillation, is triggered by deep convection around the western North Pacific. Three possible dynamical mechanisms on multiple timescales responsible for the growth and decay of the quasi-biweekly PJ pattern are proposed in this study based on daily reanalysis data from the Japanese 55-year Reanalysis for the 1958‒2021 period. First, the eastward-propagating wave energy associated with the quasi-biweekly circumglobal teleconnection in the upstream region enters the mid-latitude North Pacific and induces the wavelike barotropic geopotential height anomalies, amplifying the magnitudes of three mid-latitude centers of the PJ pattern by about 40% through their linear constructive interference. Secondly, the barotropic feedback forcing of both high-frequency and low-frequency transient eddies triggered by the pronounced meridional SST gradient over the mid-latitude Pacific is beneficial to the development and persistence of the PJ-related centers to the east of Japan and around the Bering Strait, whereas it damps the PJ-related center in the Gulf of Alaska, increasing the amplitude difference between the former two centers and the latter center. Such feedback forcing also leads to the asymmetry of the positive and negative PJ events. Thridly, dry energy conversion from the background atmospheric circulation and the moist process due to the convective heating over the western North Pacific are both efficient enough to energize the PJ pattern in the developing and mature stages, indicating that the quasi-biweekly PJ pattern can be viewed as a convectively coupled dynamical mode.
Effect of build orientation and post-curing of (meth)acrylate‐based photocurable resin fabricated by stereolithography on the mechanical behavior from quasi-static to high strain rate loadings
Authors: Zirak Nader , Shirinbayan Mohammadali , Saddaoui Ouiza , Mammeri Amrid , Azzouz Kamel , Benfriha Khaled , Tcharkhtchi Abbas , Fitoussi Joseph 
Published: September 2022

Abstract
Stereolithography (SLA) is becoming an important fabrication method among the different additive manufacturing (AM) techniques. This study investigates the effect of high strain rate on mechanical behavior, considering the fact that, materials can be shown different mechanical properties under rapid straining compared to quasi-static loading. In addition, the role of polymerization as a determining factor in the final mechanical properties of the SLA parts is indicated. Regarding, samples based on urethane dimethacrylate resin material were printed in different directions (θ = [0-90]) and post-treatment was performed with respect to the UV and UV with temperature. The effect of high strain rate was analyzed through a servo-hydraulic machine, monotonic, and interrupted tensile tests ranging from 0.3 to 117.4 s-1. Scanning electron microscopy (SEM) was applied to analyze the failure surface characteristics. The results show the important effect of strain rate on mechanical properties such that by increasing the strain rate, yield stress and ultimate stress were increased. Furthermore, investigation of the strain rate sensitivity during the different steps of the failure indicates more sensitivity of the non-linear zone of the stress-strain curve with strain rate.
The use of the rapid unequal interval interpolation method ( RUIIM) for a non-RTCP NC program and five-axis machining
Authors: chen Wei-Chih , Lin Tzu-Kuan , Teng Hsing-Ming , Tsao chung-chen 
Published: September 2022

Abstract
In industries such as electronics 3C, medical, aviation and energy, multi-axis machining machines are an important element of modern production systems, especially for post-processing, which necessitates the safe operation of control equipment. Some controller and software for five-axis simultaneous machining only interpolate equidistantly between cutter location (CL) points and do not support post-processing using a rotation tool center point (RTCP) so the machining path of the tool has manufacturing errors (overcutting or undercutting) and the workpiece fails to be machined to the correct size. Depending on the maximum allowable error (δMax), this study uses rapid unequal interval sub-point interpolation to perform sub-point interpolation on the original path of the five-axis machining machine, and the sub-point values are output in the computer numerical control (CNC) machining program in C# programming language. The tool tip is then moved to the desired machining position. Finally, an example involving helical milling of a curved surface is used to verify the feasibility of the rapid unequal interval interpolation method (RUIIM). The experimental results show that rapid unequal interval sub-point interpolation gives a more accurate original path that is generated in the CAM and a faster processing speed for the entire controller so the operation speed is increased.
Characterization of a new Komagataeibacter intermedius isolate: nanocellulose production, whole-genome analysis and genetic tractability studies
Authors: Cannazza Pietro , Rissanen Antti J , Guizelini Dieval , Minardi Carlotta , Losoi Pauli , Molinari Francesco , Romano Diego , Mangayil Rahul 
Published: September 2022

AbstractBacterial nanocellulose (BC) is a highly versatile biopolymer currently pursued as a material of choice in varied themes of biomedical and material science research fields. With the aim to extend the biotechnological applications, the genetic tractability of the BC producers within theKomagataeibactergenus and its potential as an alternative host chassis in synthetic biology have been extensively studied, albeit in modelKomagataeibacterspp. Here, we present a novelK. intermediusstrain capable of utilizing glucose, and glycerol sources for biomass and BC synthesis. Genome assembly identified one bacterial cellulose synthetase (bcs) operon containing the complete gene set encoding the BC biogenesis machinery (bcs I) and three additional copies (bcs II-IV). Interestingly, a gene annotated for cyclic di-GMP binding protein upstream ofbcs IIwhich has not yet been reported inKomagataeibacterspp. was identified from the genome. Investigations on the genetic tractability confirmed plasmid transformation, propagation of vectors with pBBR1 and p15A origin of replications and constitutive and inducible induction of recombinant protein inK. intermediusENS15. This study provides the first report on the genetic tractability ofK. intermedius, serving as starting point towards future genetic engineering of this strain.
De novo Biosynthesis of Vindoline and Catharanthine in Saccharomyces cerevisiae
Authors: Gao Di , Liu Tengfei , Gao Jucan , Xu Junhao , Gou Yuanwei , Pan Yingjia , Li Dongfang , Ye Cuifang , Pan Ronghui , Huang Lei , Xu Zhinan , Lian Jiazhang 
Published: September 2022

Abstract
Vinblastine has been used clinically as one of the most potent therapeutics for the treatment of several types of cancer. However, the traditional plant extraction method suffers from unreliable supply, low abundance, and extremely high cost. Here we employ synthetic biology approach to engineer Saccharomyces cerevisiae for de novo biosynthesis of vindoline and catharanthine, which can be coupled chemically or biologically to vinblastine. Based on a platform strain with sufficient supply of precursors and co-factors for biosynthesis, we reconstituted, debottlenecked, and optimized the vindoline and catharanthine biosynthetic pathways, representing the most complicated pathways ever reconstituted in microbial cell factories. Using shake flask fermentation, our engineered yeast strains were able to produce catharanthine and vindoline at a titer of 527.1 μg L-1 and 149.3 μg L-1, respectively, without accumulating detectable amount of pathway intermediates. The present study holds great promise for scalable production of vinblastine and other plant natural products.
Aspergillus flavus biomass in harvested maize in Kenya and Tanzania and its relation to aflatoxin levels
Authors: Temba Benigni Alfred , Bakari Gaymary George , Mgonja Frida George , Mushi James Richard 
Published: September 2022

Abstract
Controlling occurrence of aflatoxins in foods must go along with managing the fungi responsible for their production. Abundance and diversity of aflatoxin producing Aspergillus flavus account for accumulation of the toxins in crops, which has been a persistent threat for public health and economy in tropical developing countries. A study was conducted to investigate occurrence and level of A. flavus and relate them to aflatoxin level in maize in Kenya and Tanzania. A total of 786 maize samples were collected at harvesting in selected areas of the two countries for the analysis. Fungal abundance in the samples was expressed as amount of fungal DNA relative to maize DNA. The fungal DNA was quantified by qPCR, targeting internal transcribed space (ITS) gene whereas in maize DNA was quantified through alpha-tubulin gene. The two genes are known to be conserved. Aflatoxins were quantified using ultra-high performance liquid chromatography, coupled to ultra-high sensitivity, ultra-fast triple quadrupole, tandem-mass spectrophotometer. A flavus was detected in 88.5% of 786 tested samples and average fungal load for the samples (log host/pathogen ratio) was 5.53. Aflatoxin occurrence was positive in 31.9% of the samples at an average level of 2.3 ± 0.643 ppb. There was a positive relationship between occurrence and level if the aflatoxin B1 and presence and biomass of A. flavus was proved statistically. The findings of this study intensify the need to give substantial attention on preharvest control of A. flavus in cereal fields as an effort of controlling accumulation of aflatoxin B1 in foods.
Cancer cell-macrophage fusion increases tumor cell heterogeneity and reshapes their microenvironment
Authors: Li Mengtao , Basile John R. , Mallya Sanjay , Lin Yi-Ling 
Published: September 2022

Abstract
The trademark of cancer is the ability to evolve, which lays the grounds for progressive events such as metastasis and recurrence. Although genetic mutations and epigenetic changes have been implicated as the mechanisms, they don’t explain why many cancers develop leukocytic traits. Cell fusion between cancer and somatic cells, particularly macrophages, has been suggested as an alternative pathway for cancer cells to obtain new traits via acquiring exogenous genetic material. In this study, tumor-macrophage hybrid cells were generated, and two clones, both grew slowly yet with very different tumorigenicity, were selected for further study. Despite their very different abilities to form tumors in mice, both clones showed significant abilities to influence the tumor microenvironment. RNA-seq of the hybrid cell clones revealed the differential expression profiles of the hybrid cells that contributed to the biological behaviors. This study emphasizes the role of hybrid cells as potent environmental modifiers that aid tumor survival and evolution despite their minority status among the tumor cells. This study also provides an animal experimental platform to study cancer-myeloid fusion and a potential direction for novel therapeutic interventions.
Study on the formation mechanism of ornamentation patterns in spores and pollen as revealed by finite element method
Authors: Yang Kun-Long , Yang Zhu L. , Wang Pan-Meng 
Published: September 2022

Abstract
Spores (especially fungal and plant spores) and pollen usually adopt an approximately spheroidal shape and are characterized by variable and distinct ornamentation patterns. However, the mechanisms responsible for the formation of these patterns have not been thoroughly studied. In this study, spores and pollen were simplified into spheroidal core/shell systems. By using finite element method (FEM), the anisotropic stress-driven wrinkles on the systems were evaluated under different ratios of equatorial/polar radii and ratios of effective size/exine thickness, which were in comparison with spore and pollen ornamentation patterns of given species under electron microscopy. We demonstrated that anisotropic stress-driven wrinkles can reproduce various anisotropic patterns of spores and pollen from a mechanical point of view, suggesting that stress is definitely the primary determinant of ornamentation. The ratio of equatorial/polar radii and the ratio of effective size/exine thickness mainly govern the ornamentation patterns. This study provided new insights into the formation and evolution of spores and pollen ornamentation patterns in nature.
Clinicopathological study of surgically treated non-neoplastic diseases of the pancreas with special reference to autoimmune pancreatitis
Authors: Seki Makoto , Ninomiya Eiji , Saiura Akio , Takahashi Yu , Inoue Yosuke , Katori Masamichi , Yamamoto Noriko , Takamatsu Manabu , Kato Yo , Yamada Keiko , Matsueda Kiyoshi , Ohkura Yasuo 
Published: September 2022

Abstract
Purpose
After the popularization of serum immunoglobulin G4 (IgG4) measurement and endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) in our institute, surgical resection for non-neoplastic diseases of the pancreas became less common. Although the incidence of such false-positive cases was clarified in the 10-year period after introduction of these measures (2009–2018), these data were not compared with the 30 years before 2009 (1979–2008). This study was performed to determine the percentage of autoimmune pancreatitis (AIP) that was included during the latter period and how the numbers of false-positive cases differed between the two periods.
Methods
From 1979 to 2008, 51 patients had clinical suspicion of pancreatic carcinoma (false-positive disease). Among these 51 patients, 32 non-alcoholic patients who had tumor-forming chronic pancreatitis (TFCP) were clinically, histologically, and immunohistochemically compared with 11 patients who had TFCP during the latter 10-year period.
Results
Retrospective IgG4 immunostaining of TFCP revealed 14 (43.8%) cases of AIP in the former 30 years versus 5 (45.5%) in the latter 10 years. There were 32 (6.7%) cases of TFCP among 675 patients in the former 30 years and 11 (0.9%) among 1289 patients in the latter 10 years.
Conclusions
When the TFCP ratio of pancreatic resections and the AIP ratio of TFCPs were compared between the two periods, the TFCP ratio was 4.7% versus 0.9% and the AIP ratio was 43.8% versus 45.5%, respectively. These findings indicate that IgG4 measurement and EUS-FNA are imperative for the diagnosis of TFCP.
Autonomous and induced demand in the United States between 1960-2019: A long-run approach
Authors: Pérez-Montiel Jose A. , Sansó Andreu , Pariboni Riccardo 
Published: September 2022

Abstract
This paper presents a long-run study of the relationship between autonomous and induced demand spanning 1960–2019 for the United States. Our exercise can be considered a contribution to the burgeoning empirical literature revolving around autonomous demand-led growth models, which have displayed the potential to establish bridges not only within the Post- Keynesian community, but also between Post- Keynesian economics and other evolutionary and pluralistic approaches to economic growth. In particular, we study the long-run dynamic relationship between autonomous demand - which comprises R&D expenditures, government spending, exports and residential investment - and induced demand. Through a cointegration model with quantile-varying coefficients, we account for the possibility of changes in the relationship between the two variables of interest and demonstrate that the long-run equilibrium relationship between autonomous and induced demand is robust to exogenous shocks and changes in the parameters.
CAWE-ACNN Algorithm for Coprime Array Adaptive Beamforming
Authors: Liu Fulai , Qin Dongbao , Qin Hao , Du Yufeng , Dou Xiuquan , Du Ruiyan 
Published: September 2022

Abstract
This paper presents a coprime array robust adaptive beamforming algorithm based on attention convolutional neural network (ACNN), named as CAWE-ACNN algorithm. Firstly, a spatial attention unit and a channel attention unit are used to construct an attention network that can strengthen the features contributing to beamforming weight vector estimation from spatial and channel dimension, so beamformer output signal-to-interference-plus-noise ratio (SINR) is improved, and an ACNN model is established which is suitable for coprime array beamforming. Then, a virtual array of the coprime array is utilized to achieve direction-of-arrival (DOA) estimation, and a least squares method and a quadratic convex optimization problem are used to reconstruct the interference-plus-noise covariance matrix (INCM) and correct mismatched steering vector, respectively. Furthermore, the beamforming weight vector is calculated and it is regarded as the training label of the ACNN model, and the mapping procedure from covariance matrix to beamforming weight vector is completed. Finally, the well-trained ACNN model is utilized to obtain the beamforming weight vector. Simulation results verify the effectiveness of the proposed algorithm.
Analysis of the genetic stability of insect and herbicide resistance genes in transgenic rice lines: a laboratory and field experiment
Authors: Sun Yue , Chen Zhongkai , Chen Huizhen , Wang Chunlei , Li Bai , Qin Lu , Lin Xiaoli , Cai Yicong , Zhou Dahu , Ouyang Linjuan , Zhu Changlan , He Haohua , Peng Xiaosong 
Published: September 2022

Abstract
A lack of stability in the expression of Bacillus thuringiensisgenes (CRY) and the dialaninophosphate resistance gene (BAR) in transgenic rice plants can lead to the loss of important characters. The genetic stability of transgenic expression in high-generation lines is thus critically important for ensuring the success of molecular breeding efforts. Here, we studied the genetic stability of resistance to insect pests and herbicides in transgenic rice lines at the molecular and phenotypic levels in a pesticide-free environment. Southern blot analysis, real-time polymerase chain reaction, and enzyme-linked immunosorbent assays revealed high stability in the copy numbers and expression levels of CRY1C, CRY2A, and BAR in transgenic lines across different generations, and gene expression levels were highly correlated with protein expression levels. The insecticide resistance of the transgenic rice lines was high. The larval mortality of Chilo suppressalis was 50.25% to 68.36% higher in transgenic lines than in non-transgenic control lines. Percent dead hearts and percent white spikelets were 16.66% to 22.15% and 27.07% to 33.47% lower in transgenic lines than in non-transgenic control lines, respectively. The herbicide resistance of the transgenic rice lines was also high. The bud length and root length ranged were 2.53 cm to 4.20 cm and 0.28 cm to 0.73 cm higher in transgenic lines than in non-transgenic control lines in the budding stage, respectively. Following application of the herbicide Basta, the chlorophyll content of the transgenic lines began to recover 2 d later in the seedling and tillering stages and 3 d later in the booting and heading stages, by contrast, the chlorophyll content of the non-transgenic lines did not recover and continued to decrease. These findings revealed high genetic stability of the resistance to insect pests and herbicides across several generations of transgenic rice regardless of the genetic background.