bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quercetin mediated TET1 expression through miR-17 induced cell apoptosis in melanoma cells
Authors: Gao Yongjian , Li Chengshun , Lin Chao , Hou Ruizhi , Xia Qianyun , Ding Dayong , Wang Dongxu , Feng Ye 
Published: February 2022

Abstract
Previous report suggested that the expression of ten-eleven translocation (TET) proteins was abnormal in different cancers. Quercetin, has been demonstrated as anti-caner role in cancer development. To analyze the expression of TETs, quercetin treated with uveal melanoma cells in the present study. Our results suggested that the expression of TET1 was increased following treated with quercetin in OCM-1, SK-MEL-1 and B16 cells. In addition, quercetin treatment induced apoptosis and inhibited invasion. To further investigate if the expression of TET1 was associate with cell growth, apoptosis, migration and invasion, knocked down and overexpressing of TET1 was constructed. The results showed that the increased expression of TET1 induced cell apoptosis, increased 5-hydroxymethylcytosine (5hmC) and inhibited cell invasion. TET1 was a target gene of miR-17. Our results showed that inhibited expression of miR-17 increased TET1 expression in OCM-1 cells. To further confirm the effect of TET1 on melanoma tumors, nude mice was used. The results indicated that quercetin treatment increased TET1 expression and inhibited tumor growth. Taken together, these results suggested that quercetin can regulate cell proliferation and apoptosis through TET1 via miR-17 in melanoma cells.
On Variation of I–V-Characteristics of p-n-Heterojunctions Under Influence of Mismatch-Induced Stress
Authors: Pankratov Evgeny L. 
Published: February 2022

Abstract
In this paper, we consider p-n-junctions, manufactured by diffusion or ion implantation in a heterostructures. We analyzed influence of existing in heterostructure missmatch induced stresses on the current-voltage characteristics of the p-n-junctions. We also introduce an analytical approach for analysis of mass and heat transfer in heterostructures with account changes of their parameters on time, as well as their nonlinearity and missmatch induced stresses. In this paper we introduce an analytical approach for prognosis of the considered processes.
Gut Microbiome of Century-Old Snail Specimens Stable Across Time in Preservation
Authors: Chalifour Bridget , Li Jingchun , Elder Leanne 
Published: February 2022

Abstract
Background
Oreohelix strigosa (Rocky Mountainsnail) is a land snail found in the talus slopes of the Rocky Mountains. The University of Colorado’s Museum of Natural History has densely sampled Oreohelix for the past century; many are preserved in ethanol and available for molecular research. While microbiome compositions in other systems are not affected by short-term field season preservation, the effects of decades-long preservation have yet to be assessed.
Results
We used 16S rRNA (ribosomal ribonucleic acid) gene amplicon sequencing to examine Oreohelix strigosa gut microbiomes from museum specimens across a 98-year range, as well as within short term preservation treatments collected in 2018. Treatment groups included samples extracted fresh, without preservation; samples starved prior to extraction; and samples preserved for 1 month, 6 months, and 9 months. General microbiome composition was similar across all years. Sample groups belonging to specific years, or specific short-term treatments, showed unique associations with select bacterial taxa. Collection year was not a significant predictor of microbial richness, though unpreserved short-term treatments showed significantly higher richness than preserved treatments. While year was a significant factor in microbiome composition, it did not explain much of the variation across samples. Other factors like location collected and drought index were also significant drivers of community composition and explained as much or more of the variability.
Conclusions
This study is the first to examine animal host associated microbiome change across a period of nearly one century. Generally, geographic location was a greater factor in shaping gut microbiome composition, rather than year collected. Consistent patterns across this temporal range indicate that historic specimens can answer many ecological questions surrounding the host associated microbiome.
Correlation Between National Surveillance and Search Engine Query Data on Respiratory Syncytial Virus Infections in Japan
Authors: Uda Kazuhiro , Hagiya Hideharu , Yorifuji Takashi , Koyama Toshihiro , Tsuge Mitsuru , Yashiro Masato , Tsukahara Hirokazu 
Published: February 2022

Abstract
BackgroundThe respiratory syncytial virus (RSV) disease burden is significant, especially in infants and patients with an underlying disease, and prophylaxis with palivizumab is recommended for these high-risk groups. Early recognition of a RSV epidemic is important for timely administration of palivizumab. We herein aimed to assess the correlation between national surveillance and Google Trends data pertaining to RSV infections in Japan.MethodsThe retrospective survey was performed between January 1, 2018, and November 14, 2021, and evaluated the correlation of national surveillance and Google Trends data. Joinpoint regression was used to analyze the data to evaluate the points at which changes in trends occurred. ResultsAs a result, a strong correlation was observed every study year (2018 [r=0.87, p<0.01], 2019 [r=0.83, p<0.01], 2020 [r=0.83, p<0.01], and 2021 [r=0.96, p<0.01]). The change-points in the Google Trends data indicating the start of the RSV epidemic in 2018 and 2021 were observed earlier than by sentinel surveillance and in 2019 and 2020 simultaneously with sentinel surveillance.ConclusionsOur data suggested that Google Trends has the potential to enable early identification of the RSV epidemic. In countries without a national surveillance system, Google Trends may serve as an alternative early warning system.
Lung muscarinic receptor occupancy by tiotropium: translational PET studies in non-human primates and humans
Authors: Cselényi Zsolt , Jucaite Aurelija , Ewing Pär , Stenkrona Per , Kristensson Cecilia , Johnström Peter , Schou Magnus , Bolin Martin , Halldin Christer , Larsson Bengt , Grime Ken , Eriksson Ulf G , Farde Lars 
Published: February 2022

Abstract
BackgroundThe aim of the present translational PET study was to estimate occupancy of tiotropium at muscarinic acetylcholine receptors (mAChR) in the lungs in vivo. The relationship between the tiotropium exposure and receptor occupancy (RO) in the lung was assessed in non-human primates (NHPs) after intravenous injection of tiotropium doses at a broad dose interval (0.1-1mg/kg). The feasibility of measuring mAChR occupancy in the human lung was then confirmed in seven healthy human subjects after inhalation of a single therapeutic dose of tiotropium (18mg). PET examinations were performed using radioligand [11C]VC-002. Occupancy in lungs was estimated using Lassen plot analysis of parametric images showing total radioligand binding (total distribution volume, VT).ResultsThere was an evident effect of tiotropium on [11C]VC-002 binding to mAChRs in lungs in both NHPs and humans. In NHPs, the occupancy was 11 to 78%, increasing in a dose dependent manner. The Lassen-plot based estimate of non-displaceable binding in NHPs was about 10% of the VT. In humans, occupancy was 6-65%, and non-displaceable binding (VND) was about 20% of total binding, VT at baseline. ConclusionsThe results demonstrated that [11C]VC-002 binds specifically to mAChRs in the lungs such that it allows for the detection and quantification of lung muscarinic receptor occupancy following administration of an intravenously administered or inhaled muscarinic antagonist drugs. The methodology has potential for dose finding and comparison of drug formulations in future applied studies. Clinical Trial Registration: ClinicalTrials.gov identifier: NCT03097380, registered: 31 March 2017, url: https://clinicaltrials.gov/ct2/show/NCT03097380
Assessment of drought tolerance in indica rice: A new method
Authors: Singh Sandeep Kumar , Katara Jawahar Lal , Parameswaran C , Jagadev Prem Narayan , Bastia Debendra Nath , Jeughale Kishor , Samantaray Sanghamitra 
Published: February 2022

Abstract
Background: Most of the rice-growing areas are exposed to the threat of drought stress due to the effects of climate change. In order to meet this challenge and support an increasing population, it is desirable to breed rice varieties with improved drought tolerance. Breeders' success in developing improved drought resistance lines depends entirely on a simple and accurate screening process. All available drought assessment methods are time consuming, labour oriented and indirect indicators of drought. Result: We explain a method which efficiently evaluates the drought tolerance degree (DTD) of lowland indica rice varieties. DTD is defined as the average of the ratio of green leaf length to the total length of the top three leaves of each rice seedling after drought treatment, and therefore takes a value from 0 to 1. To test the potentiality of the DTD method, 118 doubled haploids with two parents showing different degrees of drought tolerance were assessed, from which the DTD value has been determined. Of the 118 doubled haploids identified by the drought tolerance assessment system under severe drought stress, DH102 showed the strongest drought resistance, with the highest DTD value, and DH22 was found to be the weakest drought resistance with the lowest DTD value. Further correlation analysis revealed a high association of DTD values with RWC, leaf tip drying scores, and leaf rolling scores. Based on these characteristics, the entire population under study was divided into two main groups. PCA analysis suggested three principal components with its first principal component possessing the DTD value, RWC, leaf rolling score, leaf tip drying score, and chlorophyll content index with maximum loading score. Conclusion: This new method will help in assessing the drought tolerance of indica rice varieties. This study indicates the DTD method is simple, cost-effective, direct and relatively accurate for drought-tolerant screening of rice varieties.
A Nomogram Predictive Model Of Ileus After Radical Cystectomy
Authors: Zhang Heng , Tian Ye , An Ling-Yue , Shan Gang , Ban Yong , Luo Guang-Heng 
Published: February 2022

Abstract
BACKGROUND: The golden standard for the treatment of muscle-invasive or high-grade bladder cancer is radical cystectomy combined with various forms of urinary diversion, but postoperative paralytic ileus is an troublesome postoperative complication.The purpose of this research is to build and verify the nomogram prediction model of postoperative ileus.METHODS: From January 2015 to December 2020, the clinical data of patients who underwent radical cystectomy in the urology department of Guizhou Provincial People's Hospital for bladder cancer were collected. The risk factors related to postoperative ileus were obtained by logitic single factor and multiple factor regression analysis. Then, the prediction model of postoperative ileus was constructed based on the risk factors and its identification ability and accuracy were verified.RESULT: 252 patients were included, 51 of whom had ileus. Statistical analysis showed that preoperative creatinine level, postoperative creatinine level, and postoperative serum albumin concentration were correlated with postoperative ileus. The area under ROC curve of nomogram predictive model constructed based on the risk factors was 0.748 (95%CI 0.672-0.824). The bootstrap correction curve of the prediction model shows that the prediction accuracy of the model is close to that of the ideal model.CONCLUSION: Preoperative creatinine level, postoperative creatinine level, and postoperative serum albumin concentration were correlated with postoperative ileus and were risk factors for ileus after radical cystectomy, which could be used as predictors of clinical prediction models. The prediction model of nomogram based on these predictors can predict the probability of postoperative intestinal obstruction.
Optimal Self-assembly of Lipid Nanoparticles (LNP) in a Ring Micromixer
Authors: RIPOLL Manon , Martin Elian , Enot Mathilde , Robbe Oscar , Rapisarda Chiara , Nicolai Marie-Claire , Deliot Aurelie , tabeling patrick , Authelin Jean-René , Nakach Mostafa , Wils Pierre 
Published: February 2022

Abstract
Lipid nanoparticles (LNPs) for RNA and DNA delivery have gained considerable attention to treat a broad range of diseases and to vectorize mRNA for COVID vaccines. LNPs are produced by mixing biomolecules and lipids, which self-assemble to form the desired structure. In this domain, microfluidics shows clear advantages: high mixing quality under low stress conditions added to fast preparation. Studies of LNPs produced in micromixers have revealed, in certain ranges of flow-rates, a degradation of performances in terms of size, size monodispersivity and encapsulation efficiency. In this study we focus on the ring micromixer, well adapted to high throughputs. We reveal three regimes - side-by-side, transitionnal and highly mixed – which control the mixing performance of the device. Further, on using cryo-TEM and biochemical analysis, we show that the mixing performances are strongly correlated to the characteristics of the LNPs we produce. We underline the important role of the flow-rate ratio and propose a physical criterion, based on the onset of temporal instabilities, coupled to a stochiometric one, for producing LNPs with optimal characteristics, in term of geometry, monodispersivity and encapsulation yield. These criteria are general.
Revisiting mechanisms of the Mesoamerican Midsummer drought
Authors: García-Franco Jorge Luis , Chadwick Robin , Gray Lesley , Osprey Scott , Adams David K 
Published: February 2022

Abstract
Observations show that the seasonal cycle of precipitation in parts of southern Mexico and Central America exhibits a bimodal signal, known as the Midsummer drought (MSD), but there is no consensus on which processes are most relevant for the two-peak structure of the rainy season. This paper evaluates three hypotheses that could explain the MSD: the SST cloud-radiative feedback, the solar declination angle and the Caribbean LowLevel Jet (CLLJ) moisture transport hypotheses. Model experiments produced by the Met Office Hadley Centre (MOHC) for CMIP6 as well as ERA5 reanalysis data are used to critically assess the predictions of each hypothesis. The simulations capture the double peak signal of precipitation well and reasonably simulate the spatial and temporal variations of the MSD and other relevant climate features such as the CLLJ. Evidence from our analysis suggests that the Eastern Pacific SSTs do not increase in late summer in ERA5 data and only slightly increase in the simulations. More importantly, the Eastern Pacific SST variability in ERA5 and in the model experiments cannot explain the differences in the seasonality of precipitation. The net shortwave radiation at the surface shows a two-peak seasonal cycle; however, this behaviour appears to result from a strong anti-correlation of the incoming shortwave and convective activity due to cloud radiative-effects. There was no evidence found by this study of a causal link in which absorption of shortwave energy forces precipitation variations, as suggested by the solar declination angle hypothesis. The moisture convergence, CLLJ and the precipitable water vapor variations best explain the characteristics of the observed and simulated MSD, particularly for the onset of the MSD. The diagnosed variations of moisture convergence, which are synchronous with the timing of the MSD, point to a dynamic mechanism in which the low-level inflow from the Caribbean is more important for the MSD than other radiative mechanisms.
Morphological and Hemodynamic Differences Between Ruptured and Unruptured Vertebral Artery Dissecting Aneurysms: A Two-Center Retrospective Study
Authors: Wei Heng , Yao Kun , Tian Qi , Han Shoumeng , Gao Wenhong , Han Wenrui , Liu Sheng , Wang Guijun , Chen Qianxue , Li Mingchang 
Published: February 2022

Abstract
Purpose
The morphological and hemodynamic features of patients with vertebral artery dissecting aneurysms (VADAs) are yet unknown. This study sought to elucidate morphological and hemodynamical features of patients with ruptured and unruptured VADAs.
Methods
This study recruited 31 patients with unruptured VADAs and 21 patients with ruptured VADAs who were treated at two hospitals. We collected data on their clinical, morphological, and hemodynamic characteristics. After reconstructing a patient-specific model, computational fluid dynamics (CFD) was performed. Binary logistic regression was used to analyze significant parameters and identify independent risk factors. Receiver operating characteristic (ROC) analysis identified cut-off values separating ruptured from unruptured aneurysms for each parameter.
Results
Three hemodynamic parameters were observed to be significantly different between ruptured and unruptured VADAs: intra-aneurysmal pressure (IAP), wall shear stress (WSS), and normalized pressure (NP). However, no morphological parameters had a significant difference between ruptured and unruptured VADAs. Binary logistic regression analysis revealed that IAP (AUC = 0.9662, 95% CI 1.014–1.068) and WSS (AUC = 0.7873, 95% CI 1.124–16.859) were independently significant variables in the hemodynamics model.
Conclusion
This study indicated significant hemodynamic differences but no significant morphological difference between ruptured and unruptured VADAs. Hemodynamic parameters of IAP and WSS are important in discriminating VADAs rupture status. These results should be confirmed through future larger studies.
Outcomes of Endoscopic Mucosal Resection for Large Superficial Non-Ampullary Duodenal Adenomas
Authors: Amoyel Maxime , Belle Arthur , Dhooge Marion , Ali Einas Abou , Pellat Anna , Hallit Rachel , Terris Benoit , Prat Fréderic , Chaussade Stanislas , Coriat Romain , Barret Maximilien 
Published: February 2022

Abstract
Background
Endoscopic mucosal resection (EMR) is the recommended treatment for superficial non-ampullary duodenal epithelial tumors larger than 6 mm. This endoscopic technique carries a high risk of adverse events. Our aim was to identify the risk factors for adverse events following EMR for non-ampullary duodenal adenomas.
Methods
We retrospectively analyzed a prospectively collected database of consecutive endoscopic resections for duodenal lesions at a tertiary referral center for therapeutic endoscopy. We analyzed patients with non-ampullary duodenal adenomas ≥ 10mm resected by EMR, and searched for factors associated with adverse events after EMR.
Results
167 duodenal adenomas, with a median (range) size of 25 (10–90) mm, were resected by EMR between January 2015 and December 2020. Adverse events occurred in 37/167 (22.2%) after endoscopic resection, with 26/167 (15.6%) delayed bleeding, 4/167 (2.4%) immediate perforation and 4/167 (2.4%) delayed perforation. In logistic regression, the size of the lesion was the only associated risk factor of adverse events (OR = 2.81, 95%CI [1.27; 6.47], p = 0.012). Adverse events increased mean hospitalization time (7.7 ± 9 vs. 1.9 ± 1 days, p < 0.01). None of the currently recommended preventive methods, particularly clips, affected the adverse event rate.
Conclusions
EMR of centimetric and supracentimetric duodenal adenomas carries a high risk of adverse events, increasing with the size of the lesion and with no benefit from any preventive method. These results suggest that these procedures should be performed in expert centers, and underline the need for novel endoscopic tools to limit the rate of adverse events.
Comparison of Efficacy of SolyxTM Single Incision Sling and TO-TVT for Female Stress Urinary Incontinence
Authors: Li Shuaishuai , Ashok Raj , Xue Ning , Zhao Fangzheng , Chen Rui , Zhu Haitao 
Published: February 2022

Abstract
Introduction To compare the efficacy, safety, and complications of the trans-obturator mid-urethral sling (TO-TVT) and SolyxTM single-incision sling for treatment of female SUIMethods The clinical data of 92 patients who underwent urinary incontinence surgery in the Affiliated Hospital of Xuzhou Medical University were retrospectively analyzed. The groups were divided on surgical techniques such as TO-TVT and the Solyx single-incision sling groups. Preoperative evaluation included patient's history, urodynamic evaluation, clinical examination results, I-QOL, and UDI-6 scores. The operative time, intraoperative blood loss, complications, and hospital stay were recorded. At six months follow-up, the patient was re-evaluated for I-QOL, UDI-6 scores, and urodynamics and clinical examinations. The PGI-I questionnaire assessed subjective cure rates at six months postoperatively. Results Patients in the TO-TVT and Solyx single-incision sling groups had similar subject cure rates six months after surgery. There was no statistical significance in intraoperative blood loss, length of hospital stay, I-QOL and UDI-6 scores between the two groups except for operation time .There was no significant difference in the incidence of intraoperative and postoperative complications between the two groups.Conclusion In terms of the effect and complications, the TO-TVT is similar to SolyxTM single incision sling. Nevertheless, SolyxTM single incision sling is shorter in operation time, more minimally invasive, and has a definite curative effect worthy of clinical promotion.
Vaccination Hesitancy On Introduction Of COVID 19 Vaccine In Pakistan: A Web-Based Cross Sectional Study April 2021
Authors: Sawani Sobiya , Siddiqui Rehana , Usmani Bilal Ahmed , Azam Syed Iqbal , Siddiqui Durr E Amna , Khwaja Hajra , Karim Sehrish 
Published: February 2022

Abstract
Background: Vaccination against COVID-19 is one of the control measures for an ongoing COVID-19 pandemic. Vaccine acceptance is known to be low in Pakistani population, we identified the factors associated with vaccine hesitancy (VH) for COVID-19 at the time of first introduction of this vaccine for elderly and health care workers. Methods: An online survey was launched using a google form during March to April 2021 to assess VH in adult (> 18 years) Pakistani population. The survey form determined VH individuals who declined to questions on getting vaccinated and maintained a perspective of reluctance to vaccinate. In addition to sociodemographic characteristics, observation of standard precautions, and sources of information on COVID-19 vaccine were inquired. Results: Of the 278 responses 80% of the study population was less than 45 years of age, 68% were males, 91% had 16 or more years of education, 39% were in health care profession, 81% reported consistent use of face masks in public places, 68% sanitized hands each time, 35% always avoided social gatherings, 24% had received vaccination, 20% had suffered from COVID-19, and 45.7% were vaccine hesitant. At multivariable level, being health care worker, increasing mean precautionary score, not being vaccinated against COVID-19 and those with concerns for vaccine safety were the contributing factors for VH. Participants who presumed COVID-19 vaccine was safe were 90% less hesitant to get COVID-19 vaccine (Adjusted Prevalence Ratio: 0.1; 95% CI 0.09, 0.29) than who were uncertain on vaccine safety.Conclusion: Reliable safety information on COVID-19 vaccine and trust building influenced VH, to mass vaccinate Pakistani population to control the pandemic.