bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-transcriptome analysis of periodontal tissue and construction of immune-related competitive endogenous RNA network
Authors: Zhao Quanquan , Wen Jing , Ouyang Xiangying , Liu Jianru , Liu Wenyi , Zhang Shengnan , Lv Peiying , Lou Xinzhe 
Published: May 2022

Abstract
Background
In periodontitis, noncoding RNAs plays a regulatory role in the immune microenvironment through competitive endogenous RNA. We aimed to profile noncoding RNA expression and interaction in periodontal tissue and identify key regulators in the immune microenvironment in periodontitis.
Methods
Five inflamed periodontal tissue and five healthy gingivae were collected for whole-transcriptome sequencing. Differential gene, functional enrichment, and protein-protein interaction network analysis were performed to explore the function of differentially expressed genes. CIBERSORTx was used to analyze level of immune cell infiltration in the periodontal tissue. An immune-related competitive endogenous RNA network was constructed and expression of key regulators in the network was validated.
Results
Compared with healthy gingiva, 200 mRNAs, 90 long noncoding RNAs, 65 microRNAs, and 518 circular RNAs were differentially expressed, and cell chemotaxis was significantly enhanced in inflamed periodontal tissue. Immune cell infiltration analysis showed that neutrophils, macrophages M1, T follicular helper cells, and naive B cells were significantly increased in periodontitis. Key regulators including JUN, FOS, THBS1, KLF2, WIF1, were identified and their expression was then validated.
Conclusion
We constructed an immune-related competitive endogenous RNA network in periodontal tissue, which provided new insights into immune homeostasis in periodontitis and laid a foundation for further study of noncoding RNAs. Key regulators in this network may be promising targets for future periodontitis treatment.
Forecasting homogenized cloud cover time series over Greece using generalized regression neural networks
Authors: Tsiotas Georgios 
Published: May 2022

Abstract
Cloud cover is an important meteorological parameter that affects the earth’s ra-diative energy balance and precipitation, playing a major role in the hydrologicalcycle. Long records of cloud cover data are the result of human estimations madeduring the synoptic observation hours at each meteorological station. Here, adoptinga homogenization methodology of the cloud cover time series of the Hellenic NationalWeather Service (HNMS) network and using monthly average cloud cover time series,we analyze 6 meteorological stations in the region of Crete, Greece for a period be-tween 1975 and 2004. To analyze these time series data, we propose the generalizedregression neural network (GRNN), a special type of neural network. By taking ad-vantage of several GRNN properties (i.e., a single design parameter and fast learning)and by incorporating several design strategies, the algorithm investigates effectivelythe possible seasonal and trend patterns found in cloud cover time series data. Fore-casting experiment results implemented under various strategies and time horizonsreveal that the GRNN methods outperforms previously adopted forecasting methodbased on exponential smoothing and ARIMA methodology. Our results reveal thatthe selected GRNN specifications may vary across monitoring stations and forecasting horizons.
Estimation of bed net coverage indicators using a national mobile phone survey in Tanzania
Authors: Worges Matt , Kamala Benjamin , Yukich Joshua , Chacky Frank , Lazaro Samwel , Dismas Charles , Aroun Sijenun , Ibrahim Raya , Khamis Mwinyi , Gitanya Peter , Mwingizi Deodatus , Metcalfe Hannah , Deku Sena , Dadi David , Serbantez Naomi , Loll Dana , Koenker Hannah 
Published: May 2022

Abstract
Background: Monitoring and surveillance of bed net coverage indicators is critical, particularly due to threats in maintaining high population access to effective bed nets, but usually occurs every 2-3 years through large-scale national household surveys. However, the rapid growth of mobile phone ownership in Tanzania has resulted in mobile phone-based survey methodologies emerging as an alternative to household surveys.Methods: A national mobile phone survey was conducted in early 2021 with focus on bed net ownership and access. Half of the sample target was contacted through a standard random digit dial methodology and the remaining half was reached through a voluntary opt-in respondent pool. Both sampling approaches used an interactive voice response survey conducted in Kiswahili.Results: The response rate was approximately 1.5% across the combined sampling approaches. Population access (i.e., the percent of the population that could sleep under a bed net, assuming one bed net per two people) varied from a regionally adjusted low of 48.1% (Katavi) to a high of 65.5% (Dodoma). The adjusted percent of households that had a least one bed net ranged from 54.8% (Pemba) to 75.5% (Dodoma); the adjusted percent of households with at least one bed net per 2 de facto household population ranged from 35.9% (Manyara) to 55.7% (Dodoma). The estimates produced by both sampling approaches were generally similar, differing by only a few percentage points. An analysis of differences between estimates generated from the two sampling approaches showed minimal bias when considering variation across the indicator for households with at least one bed net per two de facto household population.Conclusion: Mobile phone surveys can provide rapid estimates of bed net coverage in Tanzania and may serve as a suitable source of information between large-scale household surveys. The results generated by this survey show that overall bed net access in the country appears to be lower than target thresholds. The results suggest that bed net distribution is needed in large sections of the country to ensure that coverage levels remain high enough to sustain protection against malaria for the population.
Improved preoperative risk stratification in endometrial carcinoma patients: External validation of the ENDORISK Bayesian network model in a large population-based case series
Authors: Grube Marcel , Reijnen Casper , Lucas Peter JF , Kommoss Friedrich , Kommoss Felix KF , Brucker Sara Y , Walter Christina B , Oberlechner Ernst , Krämer Bernhard , Andress Jürgen , Neis Felix , Staebler Annette , Pijnenborg Johanna MA , Kommoss Stefan 
Published: May 2022

Abstract
PurposePreoperative risk stratification of newly diagnosed endometrial carcinoma (EC) patients has been hindered by only moderate prediction performance for many years. Recently ENDORISK, a Bayesian network model, showed high predictive performance. It was the aim of this study to validate ENDORISK by applying the model to a population-based case series of EC patients. MethodsENDORISK was applied to a retrospective cohort of women surgically treated for EC from 2003-2013. Prediction accuracy for LNM as well as 5-year DSS was investigated. The model’s overall performance was quantified by the Brier score, discriminative performance by area under the curve (AUC).ResultsA complete data set was evaluable from 247 patients. 78.1% cases were endometrioid histotype. The majority of patients (n=156;63.2%) had stage IA disease. Overall, positive lymph nodes were found in 20(8.1%) patients. Using ENDORISK predicted probabilities, most (n=156;63.2%) patients have been assigned to low or very low risk group with a false-negative rate of 0.6%.AUC for LNM prediction was 0.851 (95% confidence interval [CI] 0.761-0.941) with a Brier score of 0.06. For 5-year DSS the AUC was 0.698 (95% CI 0.595-0.800) as Brier score has been calculated 0.09. ConclusionsWe were able to successfully validate ENDORISK for prediction of LNM and 5-year DSS. Next steps will now have to focus on ENDORISK performance in daily clinical practice. In Addition, incorporating TCGA-derived molecular subtypes will be of key importance for future extended use. This study may support further promoting of data-based decision-making tools for personalized treatment of EC.
Virtually supported penicillin allergy de-labelling during COVID-19
Authors: Ghassemian Arian , Sadi Geetanjalee , Mak Raymond , Erdle Stephanie , Wong Tiffany , Jeimy Samira 
Published: May 2022

Abstract
Background: Penicillin allergy is a commonly listed medication allergy despite rare overall incidence. Many patients erroneously have this label, which has personal, health, and societal costs. Penicillin allergy delabelling requires an oral challenge, which can be a rate limiting step in the de-labeling process; this is even more relevant with the reduction of in-person visits during the COVID-19 pandemic. Objective: To expedite penicillin oral provocation challenge and penicillin de-labeling in patients at low to moderate risk of immediate hypersensitivity reaction, given COVID-19 restrictions. Methods: Patients referred for penicillin allergy were assessed virtually. Those deemed appropriate for oral challenge based on the allergist consultant were offered the option of a virtual oral provocation challenge to oral amoxicillin in a subsequent virtual visit. Patients who agreed and were consented underwent a virtually supervised oral amoxicillin challenge during the second virtual visit. Results: Twenty-three patients, both adult and pediatric, ranging from no to significant co-morbidities were consented and underwent the virtual challenge. One hundred percent of patients were successful with no reaction after an hour post virtual oral provocation challenge with amoxicillin. Conclusion: Virtual medicine is likely to remain in the allergist’s practice. Virtually supported penicillin allergy delabelling, based on shared decision making and risk stratification, presents another pathway for penicillin allergy delabelling. Shared decision making between the allergist and patient plays a key role to determine candidacy of the appropriate patient.
Morphological brain alterations in dialysis- and non-dialysis-dependent patients with chronic kidney disease
Authors: Yu Huan , Zhang Chaoyang , Cai Yan , Wu Ning , Jia Xiuqin , Wu Jiaojiao , Shi Feng , Yang Qi 
Published: May 2022

Abstract
Objective
To 1) investigate the morphological brain-tissue changes in patients with dialysis- and non-dialysis-dependent chronic kidney disease (CKD); 2) analyze the effects of CKD on whole-brain cortical thickness, cortical volume, surface area, and surface curvature; and 3) analyze the correlation of these changes with clinical and biochemical indices.
Methods
This study included normal controls (NCs, n = 34) as well as patients with CKD who were divided into dialysis (diagnosis-dependent chronic kidney disease [DD-CKD], n = 26) and non-dialysis (non-dialysis patients who underwent cranial magnetic resonance imaging [MRI] scans [NDD-CKD], n = 26) groups. Cortical thickness, volume, surface area, and surface curvature in each group were calculated using FreeSurfer software. Brain morphological indicators with statistical differences were correlated with clinical and biochemical indicators.
Results
Patients with CKD exhibited a significant and widespread decrease in thickness and volume compared with NCs. Among the brain regions associated with higher neural activity, patients with CKD exhibited more significant morphological changes in the paracentral gyrus, transverse temporal gyrus, and lateral occipital cortex than in other brain regions. Cortical thickness and volume in patients with CKD correlated with blood pressure, lipid, hemoglobin, creatinine, and urea nitrogen levels. The extent of brain atrophy was further increased in the DD-CKD group compared with that in the NDD-CKD group.
Conclusion
Patients with CKD potentially exhibit a certain degree of structural brain-tissue imaging changes, with morphological changes more pronounced in patients with DD-CKD, suggesting that blood urea nitrogen and dialysis may be influential factors in brain morphological changes in patients with CKD.
Heterophile carbohydrate antigen N-glycolylneuraminic acid as a potential biomarker in patients with hepatocellular carcinoma
Authors: Akimoto Shuji , Tahara Hiroyuki , Yanagawa Senichiro , Ide Kentaro , Tanaka Yuka , Kobayashi Tsuyoshi , Ohdan Hideki 
Published: May 2022

Abstract
Hepatocellular carcinoma (HCC) has a high recurrence rate even after radical hepatectomy. No optimal biomarker can predict the recurrence and prognosis of HCC. We investigated whether the oncological properties of N-glycolylneuraminic acid (NeuGc) can participate in the recurrence and prognosis of HCC. We evaluated the expression of NeuGc antigen (Ag) in the HCC tissues and measured the preoperative anti-NeuGc IgG antibodies (Abs) in the sera of the patients with HCC. We compared the clinical characteristics and survival rate in the hepatectomized patients (initial; n = 66, recurrent; n = 34) with and without the NeuGc Ag or Abs. Multivariate analyses showed positive expression of NeuGc Ag in HCC tissues (Odds ratio; initial = 6.3, recurrent = 14.0) and higher titers of preoperative anti-NeuGc Ab (Odds ratio; initial = 4.9; recurrent = 3.8), which could be the predictive factors related to early recurrence post-surgery. Both the NeuGc Ag-positive and Ab-positive groups in the initial hepatectomized patients exhibited significantly shorter recurrent free survival compared to those in the negative groups. Our findings suggested that preoperative anti-NeuGc Ab titers and NeuGc Ag expression in the HCC tissues can be used as the predictive factors for the postoperative recurrence and prognosis of HCC.
Risk factors for Ex-press® surgery failure
Authors: Otsuka Mistuya , Tojo Naoki , Hayashi Atsushi 
Published: May 2022

Abstract
Purpose: To investigate the risk factors for Ex-Press® (EXP) surgery failure.Patients and Methods: This was a retrospective non-randomized study of 98 patients who had undergone EXP and were followed-up >5 years. We investigated the following nine risk factors: age, gender, hypertension, diabetes mellitus (DM), previous glaucoma surgery, type of glaucoma (primary open angle glaucoma vs. pseudo-exfoliation glaucoma), surgical methods (EXP alone vs. EXP+cataract surgery simultaneously), central corneal thickness (CCT), and preoperative intraocular pressure (IOP). We defined a successful surgery as a post-operative reduction in the IOP >20% from the preoperative IOP and <18 mmHg. We determined the risk factors using multivariate cox regression models.Results: Performing EXP significantly decreased the IOP (pre-operative: 25.2 ± 8.7, at 5 years: 11.1 ± 4.1). The success ratio of EXP was 67.4% at 5 years. We found no significant risk factors for EXP surgery failure. The p-values of the factors were age (p=0.936), gender (p=0.0587), hypertension (p=0.409), DM (p=0.967), previous glaucoma surgery (p=0.940), type of glaucoma (p=0.435) surgical methods (p=0.521), CCT (p=0.091), and preoperative IOP (p=0.082).Conclusions: No preoperative factors that could be used to predict the failure of EXP surgery were identified. EXP can be safely performed for various types of glaucoma.
Impact of opioid analgesics on the efficacy of immune checkpoint inhibitors in a lung cancer population
Authors: Yu Xiaoyuan , Zhao Li , Song Bin 
Published: May 2022

Abstract
ObjectiveA retrospective clinical study was conducted to compare the prognosis between the opioid analgesic(OA) and non-OA groups and to evaluate the effect of opioid analgesics on the efficacy of immune checkpoint inhibitors (ICIs) in the treatment of advanced lung cancer patients. In addition, a subgroup analysis of the clinical characteristics of the enrolled patients was performed to explore possible influencing factors.MethodsWe reviewed the medical records of eligible patients who received ICIs at our institution. The clinicopathological features and clinical outcomes were compared. Also, the use of OA was collected. Patient survival, incidence of immune-related adverse events (IrAEs), and other baseline variables were examined in both cohorts according to whether OA were used.ResultsA total of 132 patients were included in the study. Of them, 39 (29.5%) were in the OA-treated group. No significant differences in baseline characteristics were observed between the OA-treated and untreated groups. Combined application of OA treatment significantly shortened progression-free survival (PFS) (P < 0.001) and overall survival (OS) (P = 0.002). However, both groups experienced similar incidences and gradations of irAEs. According to multivariate analysis, OA treatment resulted in significantly worse PFS [HR = 0.200 (95% CI: 0.124, 0.324, P = 0.001)] and OS [HR = 0.275 (95% CI: 0.160, 0.473, P = 0.001)].ConclusionsClinical outcomes of ICIs were significantly diminished in a cohort of Chinese patients with advanced lung cancer receiving OA therapy.
Effects of waterlogging and elevated salinity on the allocation of photosynthetic carbon in marsh plants and soils
Authors: Li Yalei , Ge Zhenming , Xie Lina , Li Shihua , Tan Lishan 
Published: May 2022

Abstract
Background and aimCoastal marshes, termed the “blue carbon (C)” ecosystems, are vulnerable to sea-level rise (SLR) and its consequent effects. In this study, we explored the effects of changed hydrology on the accumulation and allocation of photosynthetic C within a widely distributed marsh species and rooted soils.MethodsThe plant–soil mesocosms of Phragmites australis were grown under waterlogging and elevated-salinity conditions to investigate the responses of photosynthetic C allocation in different C pools (plant organs and soils) based on 13CO2 pulse-labeling technology.ResultsWaterlogging and elevated salinity treatments decreased photosynthetic C. Both hydrological treatments reduced 13C recovery in the plant organs of P. australis while significantly increased the 13C allocation percentage in the belowground tissues. Waterlogging and slight salinity had no notable effects on 13C recovered in rhizosphere soils, while high salinity (15 and 30 ppt) significantly reduced 13C recovered in soils, indicating a decrease in C input from roots in a saline environment. Waterlogging strengthened the effects of salinity on the 13C allocation pattern, especially during the late growing season. The responses of C allocation in plant organs and rhizosphere soils to the hydrological treatments can be related to changes in nutrient, ionic concentrations and microbial biomass.ConclusionThe adaptation strategy of P. australis led to increased C allocation in belowground organs under changed hydrology. The expected SLR projection might notably decrease the total C stock and alter the C allocation pattern in the marsh plant–soil systems, due to amplifying effect of prolonged flooding and elevated salinity.
Blind nasogastric tube insertion performed within one minute or for a maximum of three attempts minimize laryngopharyngeal injury during resuscitation
Authors: Miyamoto Kazuyuki , Takayasu Hiromi , Katsuki Shino , Maeda Atsuo , Suzuki Keisuke , Nakamura Motoyasu , Hida Noriko , Sambe Takehiko , Yagi Masaharu , Sasaki Jun , Hayashi Munetaka , Dohi Kenji 
Published: May 2022

Abstract
Blind nasogastric (NG) is inserted for patients under resuscitation, however minimal trauma to the laryngopharynx can sometimes lead severe bleeding. Recently, NG tube placement under the assistance of a Video-laryngoscope (VLS) has reported. We investigated laryngopharyngeal mucosal injury associated with blind NG tube insertion and considered practical blind NG tube insertion. Patients with cardio pulmonary arrest in whom blind nasogastric tube insertion was possible within 120 s in the Blind group and those in whom it was not possible in the Difficult (Dif) group. In the Dif group, VLS-assisted NG tube insertion was performed. Success rates, insertion time, number and laryngopharyngeal mucosal injury scores were compared. Success rates in the Blind and Dif groups were 98.5% and 76.5%, and insertion times were 48.8 ± 4.0 and 54.8 ± 3.0 s, respectively. The number of insertions and injury scores in the Blind group were significantly lower than those in the Dif group, respectively. The number of insertions and insertion time both showed strong positive correlations with injury scores. Blind NG tube insertion performed within 1 min or for a maximum of two or three attempts may minimize laryngopharyngeal mucosal injury, and VLS-assisted insertion should be considered if these limits are exceeded.
Involvement of p.R72P and PIN3 Ins16bp (TP53) polymorphisms and the I157T (CHEK2) mutation in breast cancer occurrence in Burkina FasoInvolvement of p.R72P and PIN3 Ins16bp (TP53) polymorphisms and the I157T (CHEK2) mutation in breast cancer occurrence in Burkina Faso
Authors: ZOURE Abdou Azaque , DABRE Soayebo , KIENDREBEOGO Touwendpoulimdé Isabelle , ZONGO Nayi , AMEGNONA Lanyo Jospin , SOMBIÉ Herman Karim , ADICO Marc Donald Wilfried , BAKYONO Bélélé Siméon , TRAORÉ Lassina , OUEDRAOGO Teega-Wendé Clarisse , OUEDRAOGO Rogomenoma Alice , ZOHONCON Théodora M. , YONLI Albert Théophane , SAWADOGO Alexis Yobi , DJIGMA Florencia W. , SIMPORE Jacques 
Published: May 2022

Abstract
Background: The TP53 and CHEK2 genes have been described as breast cancer susceptibility genes and some of their polymorphisms have been associated with an increased risk of breast cancer in certain populations. The objective of this study was to investigate the p.R72P and PIN3 Ins16bp (TP53) polymorphisms and the I157T (CHEK2) mutation.Methods and results: This case-control study had enrolled 144 participants including 65 cases (breast cancer patients) and 79 controls (women without breast abnormalities). The genotyping of polymorphisms was performed by ASO-PCR (Allele Specific Oligonucleotides - Polymerase Chain Reaction) and PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) techniques.The heterozygous mutated genotype (RP) of the p.R72P polymorphism was in the majority in cases (73.85%) and controls (73.42%). As for the PIN3 Ins16bp polymorphism, the homozygous wild type (A1A1) was the most represented in both cases (53.85%) and controls (60.76%). Concerning the I157T mutation, only one (01) patient was homozygous mutant (TT) and no controls had the mutation. This study found no association between these polymorphisms and the risk of breast cancer occurrence (p.R72P (OR=0.96; 95%IC (0.59-1.56); p=0.471), PIN3 Ins16bp (OR= 1.1; 95%IC (0.61-1.98); p=0.420)).Conclusion: This study showed that the mutated allele of the p.R72P polymorphism versus the wild-type allele of the PIN3 Ins16bp polymorphism were in the majority. The I157T mutation was very rare. These polymorphisms were not associated with the risk of developing breast cancer.
Intermediate monocytes expansion and homing markers expression in COVID-19 patients associate with kidney dysfunction
Authors: Dentone Chiara , Fenoglio Daniela , Parodi Alessia , Altosole Tiziana , Biagio Antonio , Bozzano Federica , Nasi Giorgia , Vena Antonio , Fabbi Marina , Ferrera Francesca , Bruzzone Bianca , Giacomini Mauro , Giacobbe Daniele Roberto , Pelosi Paolo , De Maria Andrea , Bassetti Matteo , De Palma Raffaele , Filaci Gilberto 
Published: May 2022

Abstract
Patients with severe SARS-CoV-2 infection have an overwhelming inflammatory response characterized by remarkable organs monocyte infiltration. We performed an immunophenotypic analysis on circulating monocytes in 19 COVID-19 patients in comparison with 11 naïve HIV-1 patients and 10 healthy subjects. Reduced frequency of classical monocytes and increased frequency of intermediate monocytes characterized COVID-19 patients with respect to both HIV naïve patients and healthy subjects. Intensity of C-C motif chemokine receptor 2 (CCR2) monocyte expression highly correlated with parameters of kidney dysfunction. Our data indicate that highly activated monocytes of COVID-19 patients may be pathogenically associated to the development of renal disease.