bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sakuranetin exerts anticonvulsant effect in bicuculline‐induced seizures
Authors: Vicente‐Silva Wilson , Silva‐Freitas Francisca Rayanne , Beserra‐Filho José Ivo Araújo , Cardoso Gabriela Nascimento , Silva‐Martins Suellen , Sarno Tamires Alves , Silva Sara Pereira , Soares‐Silva Beatriz , dos Santos José Ronaldo , da Silva Regina Helena , Prado Carla Máximo , Ueno Anderson Keity , Lago João Henrique Ghilardi , Ribeiro Alessandra Mussi 
Published: February 2022

AbstractEpilepsy is a chronic neurological disorder characterized by an abnormal, spontaneous, and synchronized neuronal hyperactivity. Therapeutic approaches for controlling epileptic seizures are associated with pharmacoresistance and side effects burden. Previous studies reported that different natural products may have neuroprotector effects. Sakuranetin (SAK) is a flavanone with antiparasitic, anti‐inflammatory, antimutagenic, antiallergic, and antioxidant activity. In the present work, the effect of SAK on seizures in a model of status epilepticus induced by bicuculline (BIC) in mice was evaluated. Male Swiss mice received an intracerebroventricular injection (i.c.v.) of SAK (1, 10, or 20 mg/kg—SAK1, SAK10, or SAK20). Firstly, animals were evaluated in the open field (OF; 20 min), afterwards in the elevated plus maze (EPM) test (5 min). Next, 30 min prior the administration of BIC (1 mg/kg), mice received an injection of SAK (1 or 10 mg/kg, i.c.v.) and were observed in the OF (20 min) for seizures assessment. After behavioral procedures, immunohistochemical analysis of c‐Fos was performed. Our main results showed that the lowest doses of SAK (1 and 10 mg/kg) increased the total distance traveled in the OF, moreover protected against seizures and death on the BIC‐induced seizures model. Furthermore, SAK treatment reduced neuronal activity on the dentate gyrus of the BIC‐treated animals. Taken together, our results suggest an anticonvulsant effect of SAK, which could be used for the development of anticonvulsants based on natural products from herbal source.
Effect of the upper limit stress on the fatigue properties and crack propagation processes of sandstones containing pre‐existing crack under fatigue‐creep interaction
Authors: Shi Zhanming , Li Jiangteng , Wang Ju 
Published: February 2022

AbstractTo study the effect of the upper limit stress on the fatigue characteristics and crack propagation process of jointed rock masses under the interaction of fatigue‐creep, a series of fatigue‐creep tests with different cyclic stress levels were carried out on jointed sandstone samples. Acoustic emission, scanning electron microscopy, and digital image correlation technologies were used to analyze the fatigue characteristics and crack growth process of the specimen. The results show that as the stress level increases, the deformation of the sample increases, the strength decreases, the elasticity attenuates, and the damage increases. The splitting failure of grains is weakened, the area of fatigue folds increases, and the fracture roughness increases. Besides, the crack propagation process of the sample can be divided into three stages: crack initiation, stable crack growth, and unsteady crack growth. As the stress level increases, the failure mode of the sample gradually changes from brittle to ductile.
Single‐Well Injection‐Drift Test to Estimate Groundwater Velocity
Authors: Paradis Charles , Van Ee Nathan , Hoss Kendyl , Meurer Cullen , Tigar Aaron , Reimus Paul , Johnson Raymond 
Published: February 2022

AbstractA simple algebraic equation is presented here to estimate the magnitude of groundwater velocity based on data from a single‐well injection‐drift test thereby eliminating the time‐consuming and costly extraction phase. A volume of tracer‐amended water was injected by forced‐gradient into a single well followed by monitoring of the conservative solute tracers under natural‐gradient conditions as their upgradient portions drifted back through the well. The breakthrough curve data from the single well during the drift phase was analyzed to determine the mean travel times of the tracers. The estimated mean upgradient travel distance back through the single well and the mean travel times of the tracers were used in a simple algebraic equation to estimate groundwater velocity. The groundwater velocity based on the single‐well injection‐drift test was estimated to be approximately 0.64 ft per day. Two transects of observation wells were used to monitor the natural‐gradient tracer transport downgradient of the injection well. The one‐dimensional, or dual‐well, transport of the tracer from the injection well to the nearest downgradient observation well indicated that the groundwater velocity was 0.55 ft per day. The two‐dimensional, or multi‐well, transport of the center of mass of the tracers indicated that the groundwater velocity was 0.60 ft per day; the dual‐ and multi‐well results were in excellent agreement with those from the single‐well and validated the simple algebraic equation. The new single‐well method presented here is relatively simple, rapid, and does not require an extraction phase.
Rabeprazole plus amoxicillin dual therapy is equally effective to bismuth‐containing quadruple therapy for Helicobacter pylori eradication in central China: A single‐center, prospective, open‐label, randomized‐controlled trial
Authors: Shao Qiao‐Qiao , Yu Xue‐Chun , Yu Miao , Ma Jing , Zhao Jun‐Bo , Yuan Lin , Qi Ya‐Bin , Hu Ruo‐Bing , Wei Pei‐Ru , Xiao Wei , Lan Ling , Jia Bai‐Ling , Zhang Lian‐Zhong , Ding Song‐Ze 
Published: February 2022

AbstractBackgroundAntibiotic resistance emerges as a major issue for Helicobacter pylori (H. pylori) treatment. High‐dose dual therapy has recently shown encouraging results in H. pylori eradication, but it has yet to be validated in this H. pylori highly infected area; it is also not known if this concept can be extended to antibiotics other than amoxicillin, and factors that affect the eradication. We investigate if rabeprazole plus amoxicillin or furazolidone regimens could be a first‐line therapy for H. pylori eradication, and factors that affect the curing rate.MethodsThis is a single‐center, prospective, open‐label, randomized‐controlled trial. Naive patients (n=292) were randomly treated with bismuth‐containing quadruple therapy (BQT), rabeprazole plus amoxicillin (RADT), or furazolidone (RFDT) groups. RADT and FADT use three times daily regimens. H. pylori diagnosis and eradication were determined and confirmed by 13C‐urea breath test.ResultsIn per‐protocol (PP) analysis, H. pylori eradication rate was 91.2% in BQT group, 89.6% in RADT, and 51.0% in RFDT group. In intention‐to‐treat (ITT) analysis, infection was eradicated in 86.7% of patients in BQT group, 85.8% in RADT, and 48.1% in RFDT groups, respectively. Noninferiority was confirmed between BQT and RADT groups. The incidence of side effects in BQT group was significantly higher than that in RADT group. Successful eradication was associated with lower body surface area (BSA) and low body mass index (BMI) in BQT group. Smoking and high BSA index reduced H. pylori eradication rate in RADT group.ConclusionsRabeprazole‐amoxicillin dual therapy is equally effective to the bismuth‐containing quadruple therapy for H. pylori eradication with fewer side effects and saves use of one antibiotic per each treatment. Successful eradication is also associated with low BSA and non‐smoking condition, which deserves future stratified analysis for refinement and optimization.
Defining mission statements in higher education: Towards a genre perspective
Authors: Aib Iman , Shehzad Wasima 
Published: February 2022

AbstractMission statements provide educational institutions with the legitimacy to market and promote services through positive statements. This study applied genre analysis to examine the function of promotional rhetoric in top 100 international universities' mission statements. Bhatia's rhetorical moves of promotional genre was triangulated with Pearce and David's components for mission‐writing. It was seen that international universities rely on promotional rhetorical moves to articulate their self‐concept and services. Moreover, universities actively compete against each other to secure a high position in the international ranking lists. This ensures that they obtain additional incentives that help them gain economic stability and build a reputation. Promotional genre has become a practiced culture in the professional discourse community of higher education. Hence, there is a need to allow mission statements genre membership. We suggested a genre model that can be taught and used for writing mission statements. The most recurrent moves employed suggest that the communicative purpose for mission‐writing used by international universities is to target the international market and build a public image to reel in more students, thereby improving their financial status while ensuring a good reputation. Based on this study, institutions can get guidance on designing and drafting effective mission statements.
Cord and peripheral blood erythrocyte analysis by scanning electron microscopy and flow cytometry
Authors: Manna Sayak , Biswas Payel , Haldar Rajen , Naskar Tapan Kumar , Law Sujata 
Published: February 2022

AbstractIntroductionHuman umbilical cord blood is rich in hematopoietic cells. We aimed to focus on the morphological, biochemical, membrane protein profile and surface protein expression differences of erythrocytes, isolated from cord and adult peripheral blood using techniques such as high‐resolution scanning electron microscopy (SEM), gel electrophoresis (SDS‐PAGE) and flow cytometry.MethodsAdult peripheral blood was collected from consenting adults, and umbilical cord blood was procured from consenting mothers, post‐delivery at Medical College, Kolkata. We emphasized on cord and adult peripheral blood erythrocytes' morphological variations using SEM images and protein expression by flow cytometric analysis. Some conventional biochemical analyses such as osmotic fragility of the cell membrane, haemoglobin co‐oxidation study and lipid peroxidation assay were done for supporting evidence along with membrane protein content using gel electrophoresis.ResultsOur SEM images indicated clear morphological variations in cord erythrocyte with a higher degree of cellular deformities and difference in membrane texture. Flow cytometric analysis of cord erythrocyte showed a significant difference in CD235a expression than adults. We observed an overexpression of GLUT1 and decreased expression of Band 3 in cord erythrocyte membrane. Our results also showed cord erythrocytes have low osmotic fragility, a slower rate of co‐oxidation of cord haemoglobin and a lesser lipid peroxidation level than that of adults.ConclusionCord blood erythrocytes have deeper indentations leading to higher flexibility, more oxygen‐carrying capacity and less osmotic fragility in comparison with adult erythrocytes. The expression of CD235a and Band 4.5 (GLUT 1) was significantly higher in cord erythrocytes than peripheral adult erythrocytes.
Knockdown of sphingomyelinase (NlSMase) causes ovarian malformation of brown planthopper,Nilaparvata lugens(Stål)
Authors: Shi Xiao‐Xiao , Zhang He , Quais Md. Khairul , Chen Ming , Wang Ni , Zhang Chao , Mao Cungui , Zhu Zeng‐Rong 
Published: February 2022

AbstractSphingomyelinases (SMases) are a group of enzymes that catalyse the hydrolysis of sphingomyelins into ceramides and phosphorylcholine. They have been intensively investigated for their pathophysiological roles in mammals whereas much remains unclear about their counterparts in insects. Herein we report the cloning and functional characterization of four SMase homologue genes, designatedNlSMase1‐4, from brown planthopper (BPH). The phylogenetic analysis revealed that NlSMase1 and NlSMase2 were clustered into acid SMase family, and NlSMase3 and NlSMase4 with neutral SMase family.NlSMase1,NlSMase3andNlSMase4were highly expressed in BPH females, andNlSMaes2in the 5th instar nymph. All fourNlSMaseshad the lowest transcription in BPH males.NlSMase1andNlSMase4were highly expressed in BPH ovaries, whileNlSMase2andNlSMase3in midgut and wings, respectively. Knocking‐down of eachNlSMaseindividual by RNA interference (RNAi) caused the ovarian malformation in BPH. The transcriptomic analysis revealed thatNlSMase4knockdown could strongly affect diacylglycerol (DAG)‐related metabolisms and their downstream pathways. Further, qRT‐PCR analysis ofvitellogenin(Vg) genes indicates that the DAG metabolism disorder could interrupt the essential Vg accumulation for BPH oogenesis. Our study demonstrates the vital role of NlSMases in BPH reproductive development and provides new insights into the mediated mechanism of how SMases function.
Oncolytic virus treatment differentially affects the CD56dim and CD56bright NK cell subsets in vivo and regulates a spectrum of human NK cell activity
Authors: Wantoch Michelle , Wilson Erica B. , Droop Alastair P. , Phillips Sarah L. , Coffey Matt , El‐Sherbiny Yasser M. , Holmes Tim D. , Melcher Alan A. , Wetherill Laura F. , Cook Graham P. 
Published: February 2022

AbstractNatural killer (NK) cells protect against intracellular infection and cancer. These properties are exploited in oncolytic virus (OV) therapy, where antiviral responses enhance anti‐tumour immunity. We have analysed the mechanism by which reovirus, an oncolytic dsRNA virus, modulates human NK cell activity. Reovirus activates NK cells in a type I interferon (IFN‐I) dependent manner, inducing STAT1 and STAT4 signalling in both CD56dim and CD56bright NK cell subsets. Gene expression profiling revealed the dominance of IFN‐I responses and identified induction of genes associated with NK cell cytotoxicity and cell cycle progression, with distinct responses in the CD56dim and CD56bright subsets. However, reovirus treatment inhibited IL‐15 induced NK cell proliferation in an IFN‐I dependent manner and was associated with reduced AKT signalling. In vivo, human CD56dim and CD56bright NK cells responded with similar kinetics to reovirus treatment, but CD56bright NK cells were transiently lost from the peripheral circulation at the peak of the IFN‐I response, suggestive of their redistribution to secondary lymphoid tissue. Coupled with the direct, OV‐mediated killing of tumour cells, the activation of both CD56dim and CD56bright NK cells by antiviral pathways induces a spectrum of activity that includes the NK cell‐mediated killing of tumour cells and modulation of adaptive responses via the trafficking of IFN‐γ expressing CD56bright NK cells to lymph nodes.
Utilizing the mental health nursing workforce: A scoping review of mental health nursing clinical roles and identities
Authors: Hurley John , Lakeman Richard , Linsley Paul , Ramsay Mike , Mckenna‐Lawson Stephen 
Published: February 2022

AbstractDespite rising international needs for mental health practitioners, the mental health nursing workforce is underutilized. This is in part due to limited understandings of their roles, identities, and capabilities. This paper aimed to collate and synthesize published research on the clinical roles of mental health nurses in order to systematically clarify their professional identity and potential. We searched for eligible studies, published between 2001 and 2021, in five electronic databases. Abstracts of retrieved studies were independently screened against exclusion and inclusion criteria (primarily that studies reported on the outcomes associated with mental health nursing roles). Decisions of whether to include studies were through researcher consensus guided by the criteria. The search yielded 324 records, of which 47 were included. Retained papers primarily focused on three themes related to mental health nursing clinical roles and capabilities. Technical roles included those associated with psychotherapy, consumer safety, and diagnosis. Non‐technical roles and capabilities were also described. These included emotional intelligence, advanced communication, and reduction of power differentials. Thirdly, the retained papers reported the generative contexts that influenced clinical roles. These included prolonged proximity with consumers with tensions between therapeutic and custodial roles. The results of this scoping review suggest the mental health nurses (MHNs) have a wide scope of technical skills which they employ in clinical practice. These roles are informed by a distinctive cluster of non‐technical capabilities to promote the well‐being of service users. They are an adaptable and underutilized component of the mental health workforce in a context of escalating unmet needs for expert mental health care.
Digital attrition: The negative implications of the sharing economy for the digital options of incumbent firms
Authors: Meng Tian , Ng Evelyn , Tan Barney 
Published: February 2022

AbstractOne of the key competitive advantages of sharing economy platforms stems from their superior IT capabilities, which enable operations that are more efficient and/or effective than the incumbent firms. However, given that many of the incumbents tend to be established market leaders or resource‐rich multi‐nationals, their general inability to acquire the appropriate digital options (ie, the IT capabilities that enable the launch of, and response to, competitive actions) to meet the challenge of the sharing economy platforms is puzzling. This study explores this phenomenon by posing the research question: How do the “forces at work” associated with the sharing economy paradigm impact the digital options of incumbent firms? Based on the case of Qiangsheng Taxi and how its IT capabilities have been affected by the emergence of ridesharing platforms, the evidence uncovered suggests that the sharing economy can influence the digital options of incumbent firms through a process of digital attrition, which may be induced by a blend of unfavorable contextual influences (ie, an unbalanced regulatory regime, evolving market preferences and the resourcing advantages of sharing economy platforms). A framework is inductively derived from the data collected that depicts digital attrition as a process of three phases: (a) deinstitutionalization, (b) technological incapacitation, and (c) competitive erosion. In doing so, our study suggests that digital attrition may culminate in IT‐induced competitive disadvantages for incumbent firms, which in turn, could exacerbate the unfavorable contextual influences to complete a vicious cycle that reinforces the negative influence of the sharing economy even further.
Diffusion of social science research in Nigeria
Authors: Olofinyehun Adedayo , Egbetokun Abiodun , Ayo‐Lawal Aderonke , Sanni Maruf , Oluwatope Omolayo , Yusuff Utieyineshola 
Published: February 2022

AbstractUnderstanding the research–policy nexus in developing economies requires knowing who disseminates and how social science research (SSR) is diffused. Based on survey and secondary data on a diverse set of critical stakeholders, this paper characterises the SSR diffusion landscape in Nigeria. We find that university researchers are the most active disseminators of SSR though other actors also engage in dissemination activities. Collaboration among different categories of actors, both locally and internationally, is pervasive in the system. However, online visibility of research is poor in the Nigerian SSR system. Most of the local scientific journals do not operate online, and a large share of the published SSR output is missing from widely used bibliographic databases. For a better research–policy nexus, research producers need to become skilled research advocates and policymakers need to be accessible to other actors.
Biofertilization containing Paenibacillus triticisoli BJ-18 alters the composition and interaction of the protistan community in the wheat rhizosphere under field conditions
Authors: Li Yongbin , Wang Caixia , Chen Sanfeng 
Published: February 2022

Abstract

Aims
Most studies focus on the effects of biofertilizer on the bacterial and fungal communities, and we still lack an understanding of biofertilizer on the protistan community. Here, the effects of biofertilizer containing Paenibacillus triticisoli BJ-18 on composition and interaction of the protistan community in the wheat rhizosphere were investigated.

Methods and Results
Biofertilizer application altered soil physicochemical properties and the protistan community composition, and significantly induced an alpha diversity decline. Random forecast and redundancy analysis demonstrated that nitrogenase activity and available phosphorus were the main drivers. Trichomonas classified to the phylum Metamonada was enriched by biofertilizer, and was significantly positive connected with soil nitrogenase activity and some function genes involved in nitrogen-fixation and nitrogen-dissimilation. Biofertilization loosely connected biotic interactions, while it did not affect the stability of the protistan community. Besides, biofertilizer promoted the connections of protists with fungi, bacteria, and archaea. Combined with biotic networks (protists, fungi, bacteria, and archaea) and interactions between protists and soil physicochemical properties/function genes, protists may act as keystone taxa potentially driving soil microbiome composition and function.

Significance and Impact of the Study
Overall, these results suggest that the biofertilizer is a driver of the soil protistan community, contributing to ecosystem functioning.
Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat
Authors: Wang Huan , Li Jinyan , Liang Xiaoxia , Tao Shengchang , Wu Zhanghua , Wei Gang 
Published: February 2022

Abstract

Aims
Microbial communities that inhabit plants are crucial for plant survival and well-being including growth in stressful environments. The medicinal plant, Dendrobium officinale grows in the barren soils of the Danxia Habitat. However, the microbiome composition and functional potential for growth of this plant in this environment are still unexplored.

Methods and Results
In this study, we analysed the taxonomic and functional diversity of the D. officinale Microbiome by metagenomic sequencing of both rhizosphere and endosphere samples. A total of 155 phyla, 122 classes, 271 orders, 620 families and 2194 genera were identified from all samples. The rhizospheric microbes (DXRh) were mainly composed of Proteobacteria and Acidobacteria, while Basidiomycota and Ascomycota were the most dominant phyla in root endosphere (DXRo) and stem endosphere (DXS), respectively. Most of the dominant microbial communities had been reported to have diverse functional potentials that can help plant growth and development in stressful and nutrient-deprived ecological environmental. These include plant growth promoting rhizobacteria (PGPR) such as Massilia, Pseudomonas, Bradyrhizobium, Klebsiella, Streptomyces, Leclercia, Paenibacillus, Frankia and Enterobacter in the DXRh, Tulasnella and Serendipita in the DXRo, Colletotrichum and Burkholderia in the DXS and Paraburkholderia, Rhizophagus and Acetobacter in endosphere. Analysis using the KEGG, eggNOG and CAZy databases showed that metabolic pathways such as carbohydrate metabolism, amino acid metabolism, energy metabolism, genetic information processing and environmental information processing are significantly abundant, which may be related to the survival, growth and development of D. officinale in a stressful environment.

Conclusions
We speculated that the microbial community with diverse taxonomic structures and metabolic functions inhabiting in different niches of plants supports the survival and growth of D. officinale in the stressful environment of Danxia Habitat.

Significance and Impact of the Study
This study provided an important data resource for microbes associated with D. officinale and theoretical foundation for further studies.