bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomal circZFR promotes colorectal cancer progression via stabilizing BCLAF1 and regulating the miR-3127-5p/RTKN2 axis
Authors: Chen Jiaxin , Xu Jianbin , Wang Huijuan , Chen Engeng , Meng Qing , Wang Jiawei , Zhou Wei , Shan Ge , Ju Zhenyu , Song Zhangfa 
Published: July 2022

Abstract
Background
Circular RNAs (circRNAs) have gained attention as novel biomarkers for cancer diagnosis and prognosis. Aberrant expression of circRNAs is widely involved in cancer development, whereas the function and mechanism of novel circRNAs in colorectal cancer (CRC) remain unclear.
Methods
CircZFR was identified both in CRC tissues from the GEO database and in serum exosomes by competing endogenous RNA (ceRNA) microarray. The expression of circZFR was evaluated in CRC tissues and serum by qRT-PCR and in tissue microarray (TMA) by FISH. The functional roles were confirmed using a series of in vitro assays and tumor xenografts and pulmonary/hepatic metastasis mouse models. The mechanisms of circZFR were assessed through circRNA pulldown, RNA immunoprecipitation (RIP), dual-luciferase reporter assays and rescue experiments. Lastly, TCP1-CD-QDs nanocarrier was used to carry circZFR siRNA (si-circZFR) to exam the therapeutic potential of circZFR in patient-derived xenograft (PDX) models.
Results
CircZFR was upregulated in cancer tissues and serum exosomes of CRC patients and its level was positively associated to advanced TNM stages and poor prognosis. Serum circZFR was indicative of CRC incidence, advanced-stages and metastasis. Functional experiments demonstrated that circZFR promoted CRC proliferation and metastasis but inhibited apoptosis in vitro and in vivo, exosomes with upregulation of circZFR facilitated growth and migration of cocultured CRC cells. Mechanistically, the generation of circZFR could be improved by epithelial splicing regulatory protein 1 (ESRP1) in CRC cells. CircZFR bound to BCL2-associated transcription factor 1 (BCLAF1) and inhibited its ubiquitinated degradation. Moreover, circZFR sponged miR-3127-5p to increase the expression of rhotekin 2 (RTKN2). Our TCP1-CD-QDs nanocarrier was able to carry si-circZFR and delivered them to the vasculature of CRC tissues and cells, resulting in suppressive tumor growth without obvious adverse effects in PDX models.
Conclusions
Our findings indicate that ESRP1-mediated circZFR exerts oncogenic effects on CRC development and spread through suppressing BCLAF1 degradation and regulating the miR-3127-5P/RTKN2 axis. CircZFR is a promising serum biopsy marker for CRC diagnosis and an attractive target for further therapy.
Abulia major and hypoactive delirium in COVID-19 reversed with methylprednisolone pulse therapy
Authors: Zanella Luiz Gonzaga Francisco de Assis Barros D’Elia , Zanella Luiz Gonzaga Francisco de Assis Barros D’Elia 
Published: July 2022

Abstract
Background: Sars-CoV-2 is a member of the genus Betacoronavirus like the two other coronaviruses viz. SARS-CoV (severe acute respiratory syndrome coronavirus) and MERS-CoV (Middle East respiratory syndrome coronavirus). SARS-CoV-2 infection has been associated with neuropsychiatric manifestations in acute and chronic COVID-19 (long COVID-19 syndrome), bringing social consequences and worsening people's quality of life. Case description: This article exemplifies two cases of neurological manifestations resulting from infection by SARS-CoV-2 that were reversed with methylprednisolone in a pulse therapy regimen. The first case presents a young patient with symptoms similar to those existing in patients with Alzheimer's disease, whose final and presumable diagnosis was Abulia major. The second case exemplifies an elderly person admitted to the hospital due to hypoactive delirium triggered by a urinary tract infection hypothesis. The final diagnosis was hypoactive delirium secondary to COVID-19, with urinary manifestations from SARS-CoV-2 kidney injury. Discussion/Conclusion: The purpose of this article is to warn about phenomena related to COVID-19, whose treatment can be performed with high doses of corticosteroids and with drugs that act positively on dopaminergic and serotonergic pathways. Patient exams and more information are available in the Appendix of this article.
Comparative Analysis of Complete Genome Sequences and Phenotypic Correlation of Klebsiella spp.
Authors: Sundaresan Abhirami Krishnamoorthy , Mohan Ganesh Babu Malli , Murugavel Aravind , Ramakrishnan Jayapradha 
Published: July 2022

Abstract
In recent times K. pneumoniae, a common nosocomial pathogen, tends to evolve more virulent and multidrug-resistant. Urinary tract infections by antibiotic resistant and virulent K. pneumoniae are a growing concern due to the challenging treatment strategies. Hence understanding the genome and validating the genomic profile along with pan-genome analysis will facilitate surveillance of high risk-clones of K. pneumoniae to underpin management strategies toward early detection. Thus, the present study aimed to correlate genotypic and phenotypic antimicrobial resistance and pathogenicity associated with sequence types and virulence factors in Klebsiella spp. The complete genome profile of 3 K. pneumoniae and a K. variicola were analysed in the study which was assigned to ST200, ST45, ST147 and ST of K. variicola are not known. The accessory genome comprised 66% of genes consisting of shell and cloud genes in K. pneumoniae. The phenotypic antimicrobial resistance identified kp3 to have maximum resistance and the susceptibility of environment isolate K. variicola was less resistant to the tested antibiotics. The in vitro and genomic antimicrobial resistance profiling showed concordance with all the tested antibiotics against the 4 study strains. Hypermucoviscosity was not observed for any of the test isolates; this phenotypic character matches perfectly with the absence of rmpA, rmpA2 and magA. Though the genes encoding for major virulence factors; hypermucoviscosity, yersiniabactin and allantoin metabolism were absent, significant pathogenicity and mortality were observed for kp3 isolate in the Zebrafish infection model. In contrast, the isolates predicted with the aerobactin gene were observed with least mortality rate. In the present study virulome and in vivo study illustrate the discrepancies in the virulence. To the best of our knowledge, this is the first report on the presence of ste, stf, stc and sti major fimbrial operons of Salmonella enteric serotype Typhimurium in K. pneumoniae genome, that might contribute to the virulence of kp3. Our study highlights the further necessity of genomic and phenotypic virulence markers for timely diagnosis and immediate treatment for the management of high risk K. pneumoniae clones.
The Role of Vascular-Platelet Hemostasis Disturbances in the Genesis of Respiratory Failure in Patients with COVID-19
Authors: Neymark M. I. , Epp D. P. , Nikolaeva M. G. , Momot A. P. , Proskurin S. N. , Karkavina A. N. , Kontseba V. V. , Mekshun C. V. 
Published: July 2022

The objective: to study the relationship between markers of imbalance in the platelet-vascular hemostasis system and lung damage and disease outcome in patients with COVID-19.Subjects and Methods: the study included 225 patients with the confirmed diagnosis of moderate, severe and extremely severe COVID-19. In all patients, lung damage was confirmed using computed and X-ray diagnostic methods. We studied the concentration of von Willebrand factor (vWF), activity of metalloproteinase ADAMTS-13, concentration of homocysteine, endothelin-1, thrombomodulin and the number of platelets and changes in these parameters on the 1st, 3–5th and 7–10th days from the admission to the intensive care unit (ICU). In deceased patients (n = 106), lung specimens were collected to assess the nature of morphological changes.Results. It was determined that by the 7–10th days with a lethal outcome of the disease, there was a statistically significant increase in the concentration of vWF by 22.2% (p = 0.0225), the vWF/ADAMTS-13 ratio by 2 times (p = 0.0408) and decrease in the platelet count by 52% (p = 0.0008) versus the initial stage of the study. In all the deceased, the morphological picture of lung tissue damage was characterized by destruction of alveolocytes, proliferation of connective tissue, cellular infiltration, plethora of capillaries, parietal erythrocyte thrombi in the lumen of capillaries, arterioles and venules, and obstructive erythrocyte thrombi in the lumen of vessels of lung microcirculation.Conclusion. The study showed that a possible cause of thrombosis in the lumen of vessels of lung microcirculation in the case of a lethal outcome of COVID-19 might be a primary imbalance in the vascular-platelet hemostasis characterized by a significant increase in the concentration of the vWF factor, the vWF/ADAMTS-13 ratio and a decrease in the number (consumption) of platelets by the 7–10th day of the disease versus the initial stage of the study.
Hemodynamic Reactions during Removal of Brain Tumors with the Combined Use of Fentanyl and Alpha2-Adrenoagonist
Authors: Rumyantseva M. V. , Tsentsiper L. M. , Kondratiev A. N. 
Published: July 2022

Anesthesia including drugs that act on the opioid (fentanyl) and adrenergic (clonidine or dexmedetomidine) neuroregulatory systems, creates optimal conditions for performing brain surgery.The objective: to study the effect of combination of opioid analgesic fentanyl and alpha2-adrenoagonists on stability (reactivity) and appropriate functional activity of the circulatory system.Subjects and Methods. The study included 118 patients operated on for a brain tumor. Patients were divided into groups depending on the alpha2-adrenoagonist used, its dosage and position on the operating table.Results. In all groups, the required level of neurovegetative stabilization with stable hemodynamics was achieved at all stages of the operation. Conclusion. Studies have shown that a lower dose of dexmedetomidine versus clonidine was required to achieve a central sympatho-modulatory effect.
Specific Parameters of Intraoperative Changes in the Hemostasis System during Liver Resection in Children
Authors: Novikov D. I. , Zaitsev A. Yu. , Dubrovin K. V. , Bespalov E. K. , Filin A. V. 
Published: July 2022

The objective: to determine severity of changes in the hemostasis system in children occurring at main stages of liver resection as well as the need for their intraoperative correction.Subjects and Methods. A single-center prospective pilot study of coagulogram and rotational thromboelastometry values was conducted in 25 children aged 0–11 years old with hepatoblastoma after a course of chemotherapy according to the PRETEX IV regimen who underwent hemihepatectomy.Results. There were no statistically significant differences in parameters of the ROTEM tests both when comparing groups and when comparing hemihepatectomy stages within the same age group of patients. At the beginning of the separation of the liver parenchyma INTEM: CT 200.00 (186.00; 238.00), CFT 88.00 (83.00; 139.00), MCF 54.00 (51.00; 61.00), ML 0.00 (0.00; 5.00) in Group I, CT 191.00 (179.00; 199.00), CFT 84.00 (79.00; 103.00), MCF 60.00 (58.00; 64.00), 4,00 (3.00; 9.00) in Group II, respectively, p > 0.05. After separation of the liver parenchyma INTEM: CT 201.00 (161.00; 237.50), CFT 93.00 (95.00; 112.00), MCF 54.00 (52.50; 59.50), ML 6.00 (2.00; 10.00) in Group I, CT 229.00 (201.00; 285.00), CFT 93.00 (78.00; 177.00), MCF 59.00 (49.00; 60.00), ML 5.00 (5.00; 10.00) in Group II, respectively, p > 0.05.Conclusion. After chemotherapy courses, children aged 0–11 years suffering from hepatoblastoma, maintain normal functional activity of the hemostasis system at all stages of surgical treatment. In children under 11 years of age, routine hemostasis correction with blood products and prothrombin complex factor concentrate is not required during the main stages of hemihepatectomy.
Specificity and Sensitivity of Lung Ultrasound Examination in Patients with COVID-19 Induced Pneumonia
Authors: Lakhin R. E. , Zhirnova E. A. , Shchegolev A. V. , Zheleznyak I. S. , Menkov I. A. , Chugunov A. A. 
Published: July 2022

The coronavirus infection (COVID-19) is characterized by a high incidence of pneumonia. Extensive damage, high mortality associated with COVID-19 make the rapid bedside diagnosis and dynamic monitoring of the volume and nature of lung tissue damage a challenge. Lung ultrasound examination can be used as a tool to answer it.The objective: to compare the signs detected by lung computed tomography and ultrasound and to assess the sensitivity and specificity of ultrasound in the diagnosis of pneumonia induced by COVID-19.Subjects and Methods. The observational prospective clinical study included 388 patients aged 18–75 years old; they had a confirmed diagnosis of pneumonia caused by COVID-19 or suspected COVID-19. Lung ultrasound was performed within 24 hours after computed tomography (CT) of the chest organs. During CT, pathological signs, infiltration and consolidation of the lungs were visualized which were documented by lung segments. Lung ultrasound was performed according to the Russian Protocol, ultrasound signs of B-lines and consolidation were also documented based on the projection of lung segments on the chest wall. The distributions of variables was analyzed, described and summarized. The sensitivity and specificity of ultrasound methods were evaluated on the basis of ROC analysis according to CT gold standard.Results. Bilateral involvement was found in 100% of cases. Typical CT signs of pneumonia caused by coronavirus infection were ground-glass opacity of the pulmonary parenchyma, thickened pleura, consolidation, interstitium, reticular induration, and cobblestone appearance. With ultrasound examination of the lungs and pleura, the detected signs corresponded to CT signs. B lines (multifocal, discrete or merging) and consolidation of various volumes of lung tissue were most common during ultrasound. The sign of consolidation was detected less frequently versus infiltration (p < 0.001). The sensitivity of lung ultrasound in the diagnosis of lung lesions was 95.3%, and the specificity was 85.4%, the area under the curve was 0.976 with a confidence interval of 0.961–0.991 (p < 0.001).Conclusion. The use of lung ultrasound during the COVID-19 pandemic makes it possible to identify, assess the volume and nature of lung damage. Lung ultrasound demonstrated accuracy comparable to CT of the chest organs in detecting pneumonia in patients with COVID-19.
MyARTS: Towards enhancing users’ knowledge and understanding of articles
Authors: Tengku Paris Tengku Nazatul Shima , Mohamed Hamdan Hanani , Idris Khamizatul Baizura , Mat Pardi Khalid , Ahmad Sidiki Ahmad Firdaos Syauqi , Ling Lo Mun 
Published: July 2022

MyARTS, an interactive grammar mobile game is exclusively designed according to the Theory of Variation (ToV). The game provides a variety of exercises to expose learners to different aspects of learning. The study is to examine the elements that support the design of the questions in the digital game. A qualitative content analysis was used in illuminating the elements from the theory that support the game and quantitative pre-and post-tests to see the impact of the game on learners. The features in the ToV guiding the game's tasks help learners to discern and remember the usage of articles.
Keywords: The Theory of Variation; Grammar; Interactive; Mobile-Assisted Language Learning
eISSN: 2398-4287 © 2022. The Authors. Published for AMER ABRA cE-Bs by E-International Publishing House, Ltd., UK. This is an open-access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer-review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v7i20.3430
University Administrators Retention: The Moderating roles of organisational justice and generational diversity
Authors: Harun Shariff , Ahmad Ibrahim 
Published: July 2022

Despite its wide acceptance as a theoretically important element in an organisation, organisational justice and generational diversity are neglected research areas, especially within the higher learning institutions context. Thus, this study intends to fill the knowledge gap by investigating the moderating roles of both variables towards the Malaysian Private Universities HRM practices and the administrator's retention. Two hundred seventy-two university administrators took part in the study, and empirically, the results obtained indicated that training, performance appraisal, selection, justice - management practices and distributive justice – reward are influential determinants of university administrators' retention.
Keywords: Organisational Justice; Generational Diversity; HRM Practices; University Administrators Retention
eISSN: 2398-4287 © 2022. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.
DOI: https://doi.org/10.21834/ebpj.v7i20.3451
Evaluation of the impact of asymptomatic supraventricular arrhythmias on the incidence of serious adverse events in patients on chronic hemodialysis
Authors: Lihachev-Mishchenko O. V. , Garina I. A. , Haisheva L. A. , Shlyk S. V. 
Published: July 2022

Objective: To assess the relationship of arrhythmic events such as supraventricular arrhythmias and asymptomatic arrhythmias and their long-term association with adverse events among patients undergoing chronic hemodialysis.Materials and methods: a study was conducted involving 87 patients undergoing chronic hemodialysis with a 10-year follow-up period. At the inclusion stage, sinus rhythm was registered in all patients. A total of 87 patients (47 men and 40 women; mean age 56.3±16.1 years) took part. All patients underwent Holter monitoring (HM) of the ECG after each hemodialysis procedure, 6 times in a row, and the indicators obtained during the echocardiographic study were also evaluated.Results: after evaluating the results obtained and statistically processing the data, it was found that 71.2% of patients had arterial hypertension, 30.6% had type 2 diabetes mellitus. During HM, supraventricular arrhythmias were detected in 43 patients (49.4%); these arrhythmias were short-term, asymptomatic and stopped on their own. Age (hazard ratio [HR], 1.07 per year; 95% conﬁdence interval [95% CI], 1.02 to 1.09) and right atrial enlargement (hazard ratio [HR] 4.31; 95 % CI 1.33 to 14.12), which showed a direct association with supraventricular arrhythmia in multivariate analysis. During 48 months, 67 patients died, the main cause of death of which was cardiovascular disease (58.4%). In the Cox model, the variables associated with all-cause mortality were C-reactive protein (RR, 1.03 per 1 mg/l; 95% conﬁdence interval from 1.00 to 1.08), age (RR, 1.07 per year; 95% CI, 1.00 to 1.08), supraventricular arrhythmias (RR 3.42; 95% CI, 1.34 to 7.91). Patients with supraventricular arrhythmia had a signiﬁcantly higher risk of non-fatal cardiovascular events (hazard ratio, 4.41; 95% conﬁdence interval, 2.18–8.89).Conclusion: a direct relationship has been demonstrated between the presence of supraventricular arrhythmias during chronic hemodialysis and symptomatic AF, which will develop in these patients in the future. The main aggravating factors associated with mortality in patients on dialysis were supraventricular arrhythmias, advanced age, and elevated C-reactive protein.
State-of-the-Art Deep Learning Methods on Electrocardiogram Data: Systematic Review
Authors: Petmezas Georgios , Stefanopoulos Leandros , Kilintzis Vassilis , Tzavelis Andreas , Rogers John A , Katsaggelos Aggelos K , Maglaveras Nicos 
Published: July 2022

Background
Electrocardiogram (ECG) is one of the most common noninvasive diagnostic tools that can provide useful information regarding a patient’s health status. Deep learning (DL) is an area of intense exploration that leads the way in most attempts to create powerful diagnostic models based on physiological signals.

Objective
This study aimed to provide a systematic review of DL methods applied to ECG data for various clinical applications.

Methods
The PubMed search engine was systematically searched by combining “deep learning” and keywords such as “ecg,” “ekg,” “electrocardiogram,” “electrocardiography,” and “electrocardiology.” Irrelevant articles were excluded from the study after screening titles and abstracts, and the remaining articles were further reviewed. The reasons for article exclusion were manuscripts written in any language other than English, absence of ECG data or DL methods involved in the study, and absence of a quantitative evaluation of the proposed approaches.

Results
We identified 230 relevant articles published between January 2020 and December 2021 and grouped them into 6 distinct medical applications, namely, blood pressure estimation, cardiovascular disease diagnosis, ECG analysis, biometric recognition, sleep analysis, and other clinical analyses. We provide a complete account of the state-of-the-art DL strategies per the field of application, as well as major ECG data sources. We also present open research problems, such as the lack of attempts to address the issue of blood pressure variability in training data sets, and point out potential gaps in the design and implementation of DL models.

Conclusions
We expect that this review will provide insights into state-of-the-art DL methods applied to ECG data and point to future directions for research on DL to create robust models that can assist medical experts in clinical decision-making.
A ARTE DE BEM VIVER: APROXIMAÇÕES ENTRE O ESTOICISMO E A TERAPIA COGNITIVO-COMPORTAMENTAL
Authors: Costa de Oliveira Felipe , Silva Josué Candido 
Published: July 2022

A arte de bem-viver ou felicidade (eudaimonia) foi o centro das preocupações de muitos filósofos no campo da ética. É claro que o bem-estar do indivíduo não depende só de suas escolhas, mas de uma série de fatores que fogem ao seu controle, como ter onde morar, trabalhar, as interações sociais, condições de saúde, etc. Os filósofos estoicos direcionaram suas reflexões para as modificações que o sujeito pode realizar em si mesmo através de práticas e exercícios que constituiriam, nas palavras de Foucault, as tecnologias do cuidado de si capazes de nos proporcionar a tranquilidade da alma e equilíbrio mental. Atualmente, os cuidados da alma estão entregues, em grande medida, aos psicólogos, mas ainda podemos ver muitos pontos de contato entre a filosofia estoica e certas práticas terapêuticas. Nesse artigo, apresentaremos como os estoicos pretendiam realizar uma terapia filosófica da alma e como muitas de suas ideias filosóficas permanecem como fundamentos de determinadas correntes da Psicologia, particularmente da Terapia Cognitivo-Comportamental de Albert Ellis e Aaron Beck. Além dessas aproximações, exploraremos também os pontos de afastamento e especularemos se a filosofia dos estoicos teria ainda algo a nos dizer sobre o bem-viver no mundo contemporâneo.