bluuebunny/crossref_metadata_2025_split huggingface dataset:
Berberine and Evodiamine Synergically Exert Anti-colorectal Cancer Effeccts via P38/MAPK/MAPKAPK2/HSP27 Signaling Pathway
Authors: Chen Ningning , Jiang Yifang , Yang Yi , Zhao Ziyi , Xiao Chong , You Fengming , Yan Ran 
Published: January 2022

Abstract
Objective: Combinatorial natural products have high application potential for treatment of complex diseases owing to their synergistic effects and multi-targeting effect. However, studies have not explored the therapeutic effect and the synergetic mechanisms of action combinations of natural products. The present study aimed sought to evaluate the synergistic antitumor effects of a combination of Berberine and Evodiamine, and explore the drug effect on proliferation, migration, invasion of HCT116 and RKO human colorectal cancer cells. Results: The effect of berberine and evodiamine at a specific paired dose (BER30μM, EVO 0.8μM) was explored. A combination of berberine and evodiamine had no effect on activity and proliferation of HCT116 and RKO cells. The combination regulates the cell cycle of HCT116 and RKO cells at different cell phases. Berberine mainly blocked the cell cycle at G0/G1 phase, whereas evodiamine induced cell cycle arrest at G2/M phase. The results showed that the combined effect of berberine and evodiamine does not offset each other, but plays a synergistic role in regulation of colon cancer cell cycle. Western blot analysis showed that the combination of berberine and evodiamine regulated cell cycle by downregulating expression of cdc25c and upregulating expression of p21. The combination significantly inhibited cell migration and invasion by regulating EMT related proteins, upregulating expression of E-cadherin and downregulating expression of N-cadherin. The combination of berberine and evodiamine significantly inhibited phosphorylation of P38 MAPK in HCT116 and RKO cells, and further inhibited phosphorylation of the downstream MAPKAPK2 and HSP27, thus playing a synergistic anti-colon cancer role.Conclusion: Berberine and Evodiamine exhibit synergistic antitumor effects by suppressing cell proliferation, inducing cell cycle arrest and inhibiting EMT by modulating P38MAPK /MAPKAPK2/HSP27 pathway.Significance of the study: To illustrate the potential mechanism of formula-based combination of natural products, and explore the potential applications of the combination and possible antitumor therapeutic targets.
Identification of the Glucose Metabolism Biomarkers of NASH: Weighted Gene Co-Expression Network Analysis
Authors: Chen Xingyuan , Ye Zhanhui , Chen Kequan , Xu Jiahui , Ye Liangying , Luo Yufeng , Xu Yangzhi , Liu Shiming , Yang Hui 
Published: January 2022

Abstract
Background
The genetic mechanism of glucose metabolism has not been elucidated in nonalcoholic steatohepatitis (NASH), and many genes are took part in glucose metabolism of NASH. In this study, we used the weighted gene co-expression network analysis (WGCNA) to find the key genes associated with glucose metabolism;
Methods
Data sets GSE96971 and GSE89632 from Gene Expression Omnibus (GEO) were analyzed by WGCNA. We screened the Hub gene from the GSE96971 dataset, and the selected Hub genes were verified by GSE89632 dataset. We then analyzed the dataset using the Gene Ontology (GO) term enrichment and the Kyoto Encyclopedia of Genome (KEGG) path analysis. Expression levels of the hub genes are assessed by qPCR analysis. The function of hub genes was verified by Nile Red staining and relative glucose consumption detection;
Results
The hub genes are mannosidase beta like (MANBAL), myc proto-oncogene protein (MYC), caspase 4 (CASP4), CDK5 regulatory subunit associated protein 3 (CDK5RAP3) and ZFP36 ring finger protein (ZFP36) in the datasets GSE96971 and the GSE89632. Further, these genes are mainly involved in the integral component of membrane and plasma membrane, the PI3K-AKT signaling pathway and the olfactory transduction according to the GO and KEGG results. These hub genes were significantly up-regulated in the palmitic acid (PA) cell model and methionine-choline-deficient medium (MCD) cell model. After knocking out the hub genes in PA model and the MCD model of NASH, relative glucose consumption was increased and lipid deposition was reduced compared with the control group;
Conclusions
MANBAL, MYC, CASP4, CDK5RAP3 and ZFP36 are elevated and involved in the pathogenesis of NASH. Further research on these genes are warranted.
Large spontaneous HBV DNA fluctuations and potential usefulness of a single point measurement of combined HBV DNA and quantitative HBsAg for the exclusion of HBeAg negative chronic hepatitis B: A prospective Tunisian cohort study
Authors: Chtourou Amel , Gargouri Saba , Elleuch Emna , Fki-Berrajah Lamia , Fahmi  , Taktak Awatef , Mnif Khouloud , Kassis Mondher , Hammami Adnene , Jemaa Mounir Ben , KARRAY HELA 
Published: January 2022

Abstract
Background/Aims: We aimed to describe spontaneous short-term hepatitis B Virus (HBV) DNA level fluctuations and to assess the usefulness of quantitative HBsAg (qHBsAg) in Tunisian patients with HBeAg-negative chronic HBV infection.Patients and methods: We included 174 treatment-naïve patients with chronic HBeAg-negative HBV. A one-year prospective follow-up was carried out with serial determinations of HBV DNA, alanine aminotransferase levels and qHBsAg. Patients were classified into three groups: inactive carriers (G1), patients with HBeAg negative chronic hepatitis B (CHB) (G2) and patients with indeterminate state (G3). For this latter group, a liver biopsy was indicated.Results: Only genotype D was detected. During the follow-up, 21.6% and 19.5% of patients with low initial (<2000 IU/mL) and intermediate viral load (2000-20000 IU/mL), experienced a subsequent increase in their HBV DNA levels above 2000 and 20000 IU/mL, respectively. Significant variations of HBV DNA levels (≥0.5 log10 IU/mL) were observed in 61.1% of patients at 6 months-interval. Among the 174 patients, 89 (51.1%) belonged to G1, 33 (19%) to G2 and 52 (29.9%) to G3. Fourteen patients have undergone liver biopsy, among whom 7 (50%) showed moderate to severe liver disease. Combination of HBV DNA <2000 IU/mL and qHBsAg <832 IU/mL excluded CHB in 98.4% of cases.Conclusions: This study highlights the large short-term HBV DNA fluctuations in Tunisian patients with HBeAg negative chronic HBV of genotype D. HBV DNA < 2000 IU/mL along with qHBsAg < 832 IU/mL excluded CHB in 98.4% of cases. Significant proportion of patients with indeterminate state within genotype D would have HBeAg negative CHB.
Soft Biometrics and Deep Learning: Detecting Facial Soft Biometrics Features Using Ocular and Forehead Region for Masked Face Images
Authors: Banati Urja , Prakash Vamika , Verma Rashi , Srivast Smriti 
Published: January 2022

Abstract
Soft Biometrics is a growing field that has been known to improve the recognition system as witnessed in the past decade. When combined with hard biometrics like iris, gait, fingerprint recognition etc. it has been seen that the efficiency of the system increases many folds. With the Pandemic came the need to recognise faces covered with mask in an efficient way- soft biometrics proved to be an aid in this. While recent advances in computer vision have helped in the estimation of age and gender - the system could be improved by extending the scope and detecting quite a few other soft biometric attributes that helps us in identifying a person, including but not limited to - eyeglasses, hair type and color, mustache, eyebrows etc. In this paper we propose a system of identification that uses the ocular and forehead part of the face as modalities to train our models that uses transfer learning techniques to help in the detection of 12 soft biometric attributes (FFHQ dataset) and 25 soft biometric attributes (CelebA dataset) for masked faces. We compare the results with the unmasked faces in order to see the variation of efficiency using these data-sets Throughout the paper we have implemented 4 enhanced models namely - enhanced Alexnet ,enhanced Resnet50, enhanced MobilenetV2 and enhanced SqueezeNet. The enhanced models apply transfer learning to the normal models and aids in improving accuracy. In the end we compare the results and see how the accuracy varies according to the model used and whether the images are masked or unmasked. We conclude that for images containing facial masks - using enhanced MobileNet would give a splendid accuracy of 92.5% (for FFHQ dataset) and 87% (for CelebA dataset).
When the Messenger is More Important than the Message: An Experimental Study of Evidence Use in Francophone Africa
Authors: Fillol Amandine , Sween-Cadieux Esther Mc , Larose Marie-Pier , Ventelou Bruno , Kanguem Ulrich Boris Nguemdjo , Kadio Kadidiatou , Dagenais Christian , Ridde Valéry 
Published: January 2022

Abstract
Background: Epistemic injustices are increasingly decried in global health. This study aims to investigate whether the source of knowledge influences the perception of that knowledge and the willingness to use it in francophone African health policy-making context. Methods: The study followed a randomized experimental design in which participants were randomly assigned to one of seven policy briefs that were designed with the same scientific content but with different organizations presented as authors. Each organization was representative of financial, scientific, or moral authority. For each type of authority, two organizations were proposed: one North American or European, and the other African. Results: The initial models show that there was no significative association between the type of authority and the location of the authoring organization and the two outcomes (perceived quality and reported instrumental use). Stratified analyses highlighted that policy briefs signed by the North American/European donor organization were perceived to be of lower quality than policy briefs signed by the African donor organization. For both perceived quality and reported instrumental use, these analyses found that policy briefs signed by the North American/European university were associated with higher scores than policy briefs signed by the African university whereas policy briefs signed by the North American/European regional office or international organization were associated with lower score than those signed by the African regional office of the international organization. Conclusion: The results confirm the significant influence of sources on perceived global health knowledge and the intersectionality of sources of influence. This analysis allows us to learn more about organizations in global health leadership, and to reflect on the implications for knowledge translation practices.
Recovery And The Use Of Postoperative Physical Therapy After Total Hip Or Knee Replacement
Authors: Groot L. , Latijnhouwers D.A.J.M. , Reijman M. , Verdegaal S.H.M. , Vlieland T.P.M. Vliet , Gademan M.G.J. 
Published: January 2022

Abstract
Background: To investigate the relation between recovery and postoperative physical therapy (PT) usage, including the presence of comorbidities, 6 months after total hip or knee arthroplasties (THA/TKA). Methods: Multicenter, observational study in primary THA/TKA patients who completed preoperative and 6 months postoperative assessments. The assessments included questions on PT use (yes/no and duration; long term use defined as ≥12 weeks), comorbidities (musculoskeletal, non-musculoskeletal, sensory comorbidities). Recovery was assessed with the HOOS/KOOS on all 5 subdomains. Logistic regression with long term PT as outcome was performed adjusted for confounding including an interaction term (comorbidity*HOOS/KOOS-subdomain).Results: In total, 1289 THA and 1333 TKA patients were included, of whom 95% received postoperative PT, 56% and 67% received postoperative PT ≥12 weeks respectively. In both THA and TKA group, less improvement on all HOOS/KOOS domain scores was associated with ≥12 weeks of postoperative PT (range Odds Ratios 0.97-0.99). In the THA group the impact of recovery was smaller in patient with comorbidities as non- musculoskeletal comorbidities modified all associations between recovery and postoperative PT duration (Odds Ratios range 1.01-1.05). Musculoskeletal comorbidities modified the associations between Function-in-Daily-Living-and Sport-and-recreation recovery and postoperative PT. Sensory comorbidities only had an effect on Sport-and-recreation recovery and postoperative PT. In the TKA group comorbidity did not modify the associations. Conclusion: Worse recovery was associated with longer duration of postoperative PT suggesting that PT provision is in line with patients’ needs. The impact of physical recovery on the use of long-term postoperative PT was smaller in THA patients with comorbidities. Trial registration: Registered in the Dutch Trial Registry on March 13, 2012. TRIAL ID NTR3348; registration number: P12.047. https://www.trialregister.nl/trial/3197.
Verification of the Geomagnetic Field Models Using Historical Satellite Measurements Obtained in 1964 and 1970
Authors: Soloviev Anatoly , Peregoudov Dmitry 
Published: January 2022

Abstract
In 2019, the WDC for Solar-Terrestrial Physics in Moscow digitized the archive of observations of the Earth’s magnetic field carried out by the Soviet satellites Kosmos-49 (1964) and Kosmos-321 (1970). As a result, the scientific community for the first time obtained access to a unique digital data set, which was registered at the very beginning of the scientific space era. This article sets out three objectives. First, the quality of the obtained measurements is assessed by their comparison with the IGRF reference field model. Secondly, we assess the quality of the models, which at that time were derived from the data of these two satellites and ground-based observations. Thirdly, we propose a new, improved model of the geomagnetic field secular variation based on the scalar measurements of the Kosmos-49 and Kosmos-321 satellites using modern mathematical methods.
Factors Related To Implementation of An Interprofessional Communication Concept In Thoracic Oncology: A Mixed-Methods Study
Authors: Krug Katja , Bossert Jasmin , Möllinger Sophia , Deis Nicole , Unsöld Laura , Siegle Anja , Villalobos Matthias , Hagelskamp Laura , Jung Corinna , Thomas Michael , Wensing Michel 
Published: January 2022

Abstract
Background: An innovative patient-centred interprofessional communication concept with advanced lung cancer patients (Heidelberg Milestone Communication Approach, MCA) has been developed and implemented. Role changes and interprofessional communication are challenging in a busy outpatient oncology service. The aim of the study was to assess team-related factors associated with its implementation and thoracic oncology teams’ attitudes towards interprofessional collaboration.Methods: Interviews and focus groups on implementation and interprofessional collaboration in the context of MCA were conducted with healthcare staff. The topics were analysed deductively, guided by the Professional Interactions factor of the Tailored Implementation for Chronic Diseases (TICD) framework. In addition, 3 of the 4 subscales of the validated German translation of the University of the West of England Interprofessional Questionnaire (UWE-IP-D) were collected prior to implementation of MCA (t0) with follow-up data collections at 4 months (t1), 10 months (t2) and 17 months (t3). Descriptive analysis included calculating subscale sum scores and categorizing each subscale into positive, neutral and negative attitudes. Results: Fifteen staff members participated in the interviews and focus groups. The main interprofessional interaction factors associated with implementation concerned the knowledge of the MCA and the impact of the intervention on team roles, on information sharing and on transfer processes between wards. The survey with 87 staff (44 nurses, 13 physicians, 12 psycho-social staff, 7 therapists, and 11 others) participating at least once (t0: n=20, t1: n=48, t2: n=33, t3: n=25) found heterogeneous attitudes. A positive attitude to ‘Communication and Teamwork’ was indicated by individual sum scores between 10 and 23 (M=17.7, SD=3.0). The majority of the respondents (n=48) had neutral attitudes to ‘Interprofessional Interaction’ with individual sum scores varying between 13 and 45 (positive attitude n=14, negative attitude n=22; M=28.5, SD=5.6). ‘Interprofessional Relationships’ were characterized by primarily positive attitudes (n=80) (neutral: n=6, negative attitude n=1; M=16.0, SD=3.6). There were no differences between collection times.Conclusions: Positive experiences and potential shortfalls in the implementation were observed. Future introductions of interprofessional communication concepts require further activities which should address the attitudes of healthcare professionals towards interprofessional care.Trial registration: DRKS00013469 / Date of registration: 22/12/2017.
Comparison of Alternate Part-time Patching and Pencil Push-up Training for Patients with Intermittent Exotropia
Authors: Song Desheng , Qian Jing , Chen Zhijun 
Published: January 2022

Abstract
Background

To compare the effect of alternate part-time patching and pencil push-up training on control ability in patients with intermittent exotropia.
Methods

Patients (3-7 years of age) with previously untreated intermittent exotropia were randomly assigned to receive alternate part-time patching, pencil push-up training or observation. Control ability was assessed using the Office Control Score. Stereoacuity at 40cm was evaluated with Titmus. Results were compared after 12-weeks follow-up.
Results

Ninety-two patients (28 in patching, 30 in pencil push-up and 34 in observation group) completed baseline and 12-week follow-up assessments. On the basis of 6-point scale, the mean deviation control was significantly better in patching and pencil push-up group after 12 weeks at distance (P=0.002 and 0.026, respectively). Furthermore, there was greater control changes in patching and pencil push-up groups in comparison with observation group from baseline to 12 weeks (P༜0.001;P=0.003, respectively). After 12 weeks of treatment, stereoacuity and stereoacuity changes were not significantly different between either of the intervention group and control group (P=0.140 and 0.393, respectively).
Conclusions

Based on the common office control scales, alternate part-time patching and pencil push-up training were effective treatment strategies for intermittent exotropia.
Identification of an Exosome-Related Signature Associated with Prognosis and Immune Infiltration in Breast Cancer
Authors: Guo Qiaonan , Qiu Pengjun , Pan Kelun , Chen Jianpeng , Lin Jianqing 
Published: January 2022

Abstract
Background: Exosomes are nanosized vesicles, play a vital role in breast cancer (BC) occurrence, development, invasion, metastasis, and drug resistance. Nevertheless, studies about exosome-related genes in breast cancer are limited. Besides, the interaction between the exosomes and tumor immune microenvironment (TIME) in BC are still unclear. Hence, we procced to study the potential prognostic value of exosome-related genes and their relationship to immune microenvironment in BC. Methods: 121 exosome-related genes were provided by ExoBCD database and 7 final genes were selected from the intersection of 33 differential expression genes (DEGs) and 19 prognostic genes in BC. Based on the expression levels of the 7 genes, downloaded from The Cancer Genome Atlas (TCGA) database, as well as the regression coefficients, the exosome-related signature was constructed. As a result, the patients in TCGA and GEO database were separated into low- and high- risk groups, respectively. Subsequently, R clusterProfiler package was applied to identify the distinct enrichment pathways between high-risk group and low-risk group. The ESTIMATE method was used to calculate ESTIMATE Score and CIBERSORT was applied to evaluate the immune cell infiltration. Eventually, the different expression levels of immune checkpoint related genes were analyzed between the two risk groups. Results: Results of BC prognosis vary from different risk groups. The low-risk groups were identified with higher survival rate both in TCGA and GEO cohort. The DEGs between high- and low- risk groups were found to enrich in immunity, biological processes, and inflammation pathways. The BC patients with higher ESTIMATE scores were revealed to have better overall survival (OS). Subsequently, CD8+ T cells, naive B cells, CD4+ resting memory T cells, monocytes, and neutrophils were upregulated, while M0 macrophages and M2 macrophages were downregulated in the low-risk group. At last, 4 genes reported as the targets of immune checkpoint inhibitors were further analyzed. The low-risk groups in TCGA and GEO cohorts were indicated with higher expression levels of LAG-3, CD274, TIGIT and CTLA-4. Conclusion: According to this study, exosomes are closely associated with the prognosis and immune cell infiltration of BC patients. These findings may make contributions to improve immunotherapy and bring a new sight for BC treatment strategies.
Relative Contributions of Tropical and Mid-Latitude Signals on Intraseasonal Precipitation Anomalies Over South China in Boreal Winter
Authors: Wang Lu , Jiang Jie , Li Tim 
Published: January 2022

Abstract
The southern China (SC) exhibits a strong intraseasonal precipitation variability in boreal winter, but so far the relative contributions of the tropical Madden-Julian Oscillation (MJO) and the mid-latitude intraseasonal oscillation (ISO) is unclear. This issue is addressed through a cluster analysis. The result shows that 53% of strong intraseasonal precipitation events are unrelated to the MJO. They are caused by southward propagation of a low-pressure anomaly in the lower troposphere from higher latitudes. Southerly anomalies associated with the low-pressure system transport high mean moisture from South China Sea, leading to moisture accumulation over SC. 47% of the events are accompanied by the MJO, and they can be further divided into two groups: one with enhanced MJO convection over the eastern Indian Ocean (termed as IO group), and the other over the Maritime Continent (termed as MC group). For the IO group, the SC precipitation is triggered by low-level southerly anomalies associated with an anomalous anticyclone over the western North Pacific (WNP) in association with suppressed MJO convection in situ, as well as the upper-tropospheric divergence related to a wave train excited from the MJO convection. For the MC group, both the upper-tropospheric wave train related to MJO and the southward propagation of low-pressure anomaly from higher latitudes in the lower troposphere contribute to trigger the SC precipitation.