bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leaf-venation-directed cellular alignment for macroscale cardiac constructs with tissue-like functionalities
Authors: Mao Mao , Qu Xiaoli , Zhang Yabo , Gu Bingsong , Li Chen , Liu Rongzhi , Li Xiao , Zhu Hui , He Jiankang , Li Dichen 
Published: November 2022

Abstract
Engineering functional cardiac tissues would represent a therapeutic alternative for patients with end-stage heart disease. Recapitulating the complex structural, mechanical, and electrophysiological properties of the heart is crucial to improving the utility of the engineered cardiac tissues. Here, we report a leaf-venation-directed strategy that enables the contraction and remodeling of cell-hydrogel hybrids into a highly aligned and densely packed organization in predetermined patterns. This strategy contributes to biomimetic hierarchical vasculatures with interconnected tubular structures and the improved maturation and functionality of the engineered rat and human cardiac tissues, evidenced by robust electrophysiological activity, macroscopically synchronous contractions, and upregulation of crucial maturation genes. With the mechanical support of the elastic scaffolds, functional leaf-venation-directed tissues can be assembled into 3D pre-vascularized cardiac constructs resembling the anisotropic mechanical properties of native myocardium and allowing for minimally invasive implantation. The present strategy may generate cardiac tissue constructs with multifaceted functionalities to meet clinical demands.
Association between triglyceride glucose index and subclinical left ventricular systolic dysfunction in patients with type 2 diabetes
Authors: Chen Yanyan , Fu Jianfang , Wang Yi , Zhang Ying , Shi Min , Wang Cheng , Li Mengying , Wang Li , Liu Xiangyang , Ta Shengjun , Liu Liwen , Li Zeping , Li Xiaomiao , Zhou Jie 
Published: November 2022

Abstract
Background
Triglyceride glucose (TyG) index has been considered a new biomarker for diagnosis of angiocardiopathy and insulin resistance. However, the association of TyG index with subclinical left ventricular (LV) systolic dysfunction still lacks a comprehensive exploration. The study was carried out to examine this relationship in the asymptomatic with type 2 diabetes mellitus (T2DM).
Methods
150 T2DM cases with preserved LV ejection fraction (LVEF ≥ 50%) from June 2021 to December 2021 were enrolled in this study. The subclinical LV function was evaluated through global longitudinal strain (GLS), with the pre-defined GLS < 18% as the cutoff for subclinical LV systolic dysfunction. The TyG index calculation was achieved according to ln (fasting triglycerides (mg/dL) × fasting glucose (mg/dL)/2), which was then stratified into 4 quartiles (TyG-Q).
Results
The analyses of baseline characteristics in the four TyG-Q (Q1 (TyG ≤ 8.89) n = 38, Q2 (8.89 < TyG ≤ 9.44) n = 37, Q3 (9.44 < TyG ≤ 9.83) n = 38, and Q4 (TyG > 9.83) n = 37) were conducted. A negative correlation of TyG index with GLS (r=-0.307, P < 0.001) was revealed according to correlation analysis. After the gender and age adjusted in multi-model logistic regression analysis, the higher TyG index (OR 6.86; 95% CI 2.44 to 19.30; P < 0.001, Q4 vs Q1) showed a significant association with GLS < 18%, which was still maintained after further adjustment for related clinical confounding factors (OR 5.15, 95%CI 1.13 to 23.39, p = 0.034, Q4 vs Q1). Receiver operator characteristic analysis indicated a diagnostic capacity of TyG index for GLS < 18% (area under curve: 0.678; P < 0.001).
Conclusions
Higher TyG index had a significant association with the subclinical LV systolic dysfunction in asymptomatic T2DM patients, with the potential to exert prognostic value for the progression of myocardial damage.
Effects of fructose epimers on blood lipid profile: A systematic review and meta-analysis of randomized controlled trials
Authors: Xu Cuiju , Chen Shanbin , Du Fangling , Zong Aizhen , Xu Tongcheng , You Yanli 
Published: November 2022

Abstract
OBJECTIVES
The epimers of fructose may have the benefit of regulating lipid metabolism. This systematic review and meta-analysis of randomized controlled trials (RCTs) aimed to assess the effects of fructose epimers on blood lipids, including high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), total cholesterol (TC), triglyceride (TG) and free fatty acids (FFA).
METHODS
PUBMED, EMBASE, Cochrane Library and Web of science databases were retrieved up to November 2022. We included all published RCTs concerning the effects of fructose epimers on fasting or postprandial blood lipid responses. Data were pooled with standardized mean differences (SMD). Subgroup analysis was applied to investigate the heterogeneity. Quality of literature was accessed with version 2 of the Cochrane risk-of-bias tool for randomized trials (RoB 2).
RESULTS
A meta-analysis of 6 trials including 686 participants was eligible. The pooled data revealed that single dose fructose epimers intervention significantly increase postprandial FFA levels (SMD, 0.64; 95% CI, 0.07 to 1.22; P = 0.027) but no effect on postprandial TG. The daily intervention of fructose epimers significantly ameliorated the fasting plasma HDL-C level (SMD, -0.42; 95% CI, -0.83 to -0.01; P = 0.046). In addition, fructose epimers showed an obvious but not significant effect on reducing fasting TC level (SMD, -0.13; 95% CI, -0.29 to 0.04; P = 0.135). The epimers of fructose have no observable effect on fasting LDL-C and TG levels.
CONCLUSIONS
Fructose epimers intake leads to modest improvements in blood lipid profiles. Strong and long-term randomized controlled trials are needed to confirm the certainty and sustainability of these improvements.
Application of Deep Learning Technology for Temporal Analysis of Videofluoroscopic Swallowing Studies
Authors: Jeong Seong Yun , Kim Jeong Min , Park Ji Eun , Baek Seung Jun , Yang Seung Nam 
Published: November 2022

Abstract
Background: For an objective and quantitative evaluation for videofluosroscopic swallowing study (VFSS), temporal parameters during swallowing are analyzed for both clinical and research purposes. However, because it is time-consuming and is complicated to analyze manually by clinicians, automated analysis using deep learning has been attempted. This study aimed to develop a model for automatic measurement of various temporal parameters of swallowing using deep learning.
Methods: Five-hundred and forty-seven VFSS video clips were included in this study. Seven temporal parameters were manually measured by two physiatrists as ground-truth data: oral phase duration, pharyngeal delay time, pharyngeal response time, pharyngeal transit time, laryngeal vestibule closure reaction time, laryngeal vestibule closure duration and upper esophageal sphincter opening duration. ResNet3D was chosen as the base model for deep learning of temporal parameters. Specifically, we proposed several architectural variants of ResNet3D to improve the estimation of temporal parameters. The performance of ResNet3D variants was compared with that of the VGG and I3D models which were used in the previous studies.
Results: The average accuracy of the proposed ResNet3D variants in measuring the temporal parameter of VFSS was from 0.864 to 0.981. F1 score ranged from 0.707 to 0.924, and average precision ranged from 0.643 to 0.865. In comparison with the VGG and I3D models, our model achieved the best results in terms of accuracy, F1 score and average precision values.
Conclusion: We proposed deep learning models for automatically measuring temporal parameters of VFSS. The proposed ResNet3D variants outperformed the existing models in detecting all phases of the swallowing process. Through the clinical application of this automatic model,, temporal analysis of VFSS will be easier and more accurate.
Optimal dual-energy computed tomography scan parameters to detect small- sized urinary stones and their composition
Authors: Jung Jin-Woo , Shin Jun-Bong , Choi Hyo-Jun , Pak Seongyong , Yang Hyungjin , Yoon Byung Il 
Published: November 2022

Abstract
Objective
To investigate the optimal scanning parameters of dual-energy computed tomography (DECT), which can accurately determine sensitivity (the detectability of urinary stones) and accuracy (the composition matching of urinary stones), and to apply them to clinical trials.
Methods
Fifteen urinary stones were chemically analyzed, and their chemical compositions were considered a reference standard with which we compared the uric acid (UA) and non-UA compositions determined using DECT. The urinary stones were placed inside a bolus and scanned with a dual-source CT scanner under various selected dual-energy conditions (A to X) using various solid water phantom thicknesses. These datasets were analyzed using the Siemens syngo.via software tool (integrated into the CT system) for matching the sensitivity and accuracy assessments.
Results
This study showed that 80% of the highest sensitivity (detection of urinary stones) and 92% of the highest accuracy (composition matching of urinary stones) were achieved under condition A (a collimation beam width setting of 2 × 32 mm × 0.6 mm, an automatic exposure control setting of 80/Sn140 peak kilovoltage, and a slice thickness of 0.5/0.5 mm) (P < 0.05).
Conclusion
Application of the DECT energy parameters presented in the study will help identify the sensitivity and accuracy of UA and non-UA stone analysis, even in patients with small-sized urinary stones and in conditions difficult for analysis.
Initial experience of single-incision plus one port totally laparoscopic pancreaticoduodenectomy
Authors: Cai He , zhang Man , Wang Xin , Cai Yunqiang , Peng Bing 
Published: November 2022

Abstract
Background: The use of single-incision plus one-port laparoscopic pancreaticoduodenectomy (SILPD+1) has been never reported and its safety and efficacy remain unknown. The objective of the study is to evaluate the short-term outcomes of SILPD+1 compared with conventional laparoscopic pancreaticoduodenectomy (CLPD).
Method: Fifty-seven cases of laparoscopic pancreaticoduodenectomy (LPD) were performed by a single surgical team in the Department of Pancreatic Surgery, West China Hospital, Sichuan University between November 2021, and March 2022. Among these, 10 cases of LPD were performed using a single-incision plus one-port device. Based on the same inclusion and exclusion criteria, 47 cases of LPD performed using traditional 5-trocar were included as a control group. The patient’s demographic characteristics, intraoperative, and postoperative variables were prospectively collected and retrospectively analyzed.
Results: Three men and seven women were included in the SILPD+1 group. All baseline parameters of both groups were comparable except for age. Patients were younger in the SILPD+1 group (47.2 ± 18.3 years vs 60.6 ± 11.7 years, P = 0.05) than that in the CLPD group. Compared to the CLPD group, median operation time (222.5 (208.8–245.0) vs. 305.0 (256.0-337.0) min, P < 0.001) was shorter, median postoperative VAS scores on days 1-3 were lower, and median cosmetic score (21.0 (19.0-23.5) vs. 17.0 (16.0-20.0), P = 0.026) was higher one month after the surgery in SILPD+1 group. The estimated blood loss, conversion rate, blood transfusion rate, exhaust time, time of drainage tube removal, postoperative hospital stays, and perioperative complications were comparable between the two groups.
Conclusion: In a high-volume LPD center, SILPD+1 is safe and feasible without increasing the operation time and complications. It even has the advantages of reduced postoperative pain and improved cosmetic results.
Investigation of physical and rheological properties of LDPE/HDPE/thermoplastic starch biodegradable blend films
Authors: Zeraatpishe Mohammad , Hassanajili Shadi 
Published: November 2022

Abstract
In this work, low-density polyethylene/high density polyethylene/thermoplastic starch (LDPE/HDPE/TS) films were prepared with different compositions to obtain environmentally friendly materials. LDPE/HDPE/TS blends, at ratios of 95:5:15, 90:10:15, 85:15:15, 85:15:20, 85:15:25 and 85:15:30, were processed into thin films by single-step single-screw extrusion. Fourier transform infrared (FTIR) spectra confirmed the immiscibility of matrices and TS in the blend, also in the SEM micrographs of the films no miscibility between the PE and starch was observed. in addition, the SEM micrographs showed a fairly good dispersion of TS (at low wt%) in the blend. Differential scanning calorimetry (DSC) analysis depicted an increase in the percentage of crystallinity with increasing HDPE and a decrease in the percentage of crystallinity with increasing TS. With the increase of HDPE content at low wt% of TS in the blend, an improvement was obtained in oxygen barrier property of the prepared films when compared to LD film. Water absorption of the samples decreased to some extent with increasing HDPE and the absorption increased with increasing TS. The rheological properties of LDPE/HDPE/TS blends were investigated before and after biodegradation. Results were fitted with well-known rheological models to achieve more valuable data about the effect of the composition as well degradation condition on the blend property. By adding HDPE in the presence of TS, rheological properties close to the LDPE were obtained for LDPE/HDPE/TS samples.
Effectiveness of public gardens on the insect diversity of green roofs
Authors: Hussain Raja Imran , Frank Thomas , Kratschmer Sophie 
Published: November 2022

Abstract
Rapid urbanization is among the factors, causing decrease in insect diversity. However, by offering suitable habitat, green roofs could lessen such adverse effects of urbanization. Certain factors, like a nearby public garden could be useful predictor variables to analyze at what extent green roofs can support insect communities. The aim of the study was to measure the insect diversity on intensive green roofs located close to public gardens, and more isolated green roofs within an urban setting. Insect species richness, abundance and assemblages were different on the green roofs close to public gardens compared to isolated green roofs. We can conclude that green roofs near a public garden will host more species, especially pollinators, consequently lessens urbanization's negative effect. Establishing a green roof at the right location would probably enhance biodiversity conservation in an urban setting. To properly understand how landscape factors impact insect communities on green roofs, future studies on green roofs should take public gardens into consideration.
The significance of preoperative serum fibrinogen in patients with gallbladder carcinoma after curative-intent resection: A Lasso predictive model with an extremely high predictive accuracy and a meta-analysis
Authors: Lv Tian-Run , Hu Hai-Jie , Liu Fei , Ma Wen-Jie , Jin Yan-Wen , Li Fu-Yu 
Published: November 2022

Abstract
Objective
To systematically evaluate the prognostic value of preoperative fibrinogen in surgically-treated patients with gallbladder carcinoma (GBC). Various prognostic models were established to evaluate their predictive accuracy versus the latest 8th American Joint Committee on Cancer (AJCC) staging system.
Methods
Post-surgical patients with GBC between 2010 and 2020 were identified. Time-dependent receiver operating characteristic (ROC) curve was used evaluate the diagnostic performance of fibrinogen. Lasso regression-based predictive model and Cox regression-based predictive model were developed. Model performance was evaluated via area under curve.
Results
Based on our cohort and the following meta-analysis, an elevated preoperative fibrinogen level in GBC indicated a more advanced stage and a worse prognosis. Even after propensity score matching, the recurrence rate was still significantly higher in patients with hyper-fibrinogen. The diagnostic performance of fibrinogen on overall survival was inferior to CA199 while satisfactory performance was acquired when they were combined. Lasso regression-based predictive model with eight predictors incorporated (postoperative chemotherapy, age, preoperative fibrinogen, surgical margin, node metastasis, lymph-vascular invasion, liver invasion, and T stage) showed the highest predictive accuracy (AUC: 0.9) and the latest 8th AJCC staging system has the worst predictive accuracy (AUC: 0.85).
Conclusion
An elevated preoperative fibrinogen in resected GBC indicated a more advanced stage and a worse prognosis. An established Lasso model with eight predictors included, especially the incorporation of fibrinogen, showed the most superior predictive performance than Cox-based model or the latest 8th AJCC staging system.
CDKN1C gene mutation causing familial Silver–Russell syndrome, with family pedigree
Authors: Li Jie , He Hainan , Chen Lina 
Published: November 2022

Abstract
Background: CDKN1C is a cell proliferation inhibitor that regulates the cell cycle and cell growth through G1 cell cycle arrest. CDKN1C mutations can lead to IMAGe syndrome (CDKN1C allele gain-of-function mutations lead to intrauterine growth retardation, primary adrenal insufficiency, metaphyseal dysplasia, and genital malformations). So far,There are three reported pedigrees worldwide had CDKN1C missense mutations that caused silver Russell syndrome (SRS) (p.Arg279Leu, p.Arg279Ser). The affected family members showed an SRS phenotype but did not have limb asymmetry or adrenal insufficiency. The amino acid changes in this specific region were located in a narrow functional region that contains mutations previously associated with IMAGe syndrome.
Results: We found an SRS pedigree, which was due to missense mutation affecting the same amino acid position , 279, of the CDKN1C gene, resulting in the amino acid substitution p.Arg279His (c.836G>A). Five affected family members also showed SRS phenotypes , but without limb asymmetry or adrenal insufficiency. Two affected member had diabetes mellitus. Although the SRS-related CDKN1Cmutation is in the IMAGe-related mutation hotspot region(the proliferating cell nuclear antigen (PCNA) domain), no adrenal insufficiency has been reported in this SRS pedigree. The reason may be that the location of genomic mutation and the type of missense mutation determine the phenotype.
Conclusions: Functional mutations in CDKN1C can lead to familial SRS without limb asymmetry, and some patients may have glucose abnormalities. In familial SRS patients, the PCNA region of CDKN1C should be analysed. Adrenal insufficiency should be excluded in all patients with functional CDKN1C variants.
DDIT4 mediated autophagy and ferroptosis through VDR-mTOR signaling pathway in the treatment of diabetic kidney disease
Authors: Lu Hai-tao , Jiao Yuan-yuan , Yu Tian-yu , Shi Jing-xuan , Tian Jing-wei , Zou Gu-ming , Liu Lin , Li Wen-ge , Zhuo Li 
Published: November 2022

Abstract
To determine the changes in the expression of DNA damage inducing transcription factor 4 (DDIT4) and the gene related to the activation of vitamin D receptor (VDR) in diabetic kidney disease (DKD) patients and experimental mice, and to explore the molecular mechanism of DDIT4 in the treatment of DKD. The pathological damage of kidney tissue and the molecular expression of VDR-mTOR related pathway in DKD patients and db/db mouse models were observed by periodate schiff (PAS) staining and immunohistochemistry (IHC) staining; furthermore, diabetic cell models were established in mouse immortalized kidney podocyte line MPC5 cells and mouse glomerular mesangial cell line SV40-MES-13 cells by high glucose culture, transfected with DDIT4 plasmid, observed cell morphological changes by transmission electron microscopy and laser confocal microscopy, and detected VDR/mTOR/p70s6k/4E-BP1 signal pathway protein expression by qRT-PCR and western blotting. PAS staining and IHC staining results showed that pathological damage of kidney tissue was observed in samples of DKD patients and in db/db mouse models. The results of qRT-PCR and western blotting showed that the expression of related proteins in VDR mTOR signal pathway changed. DDIT4 treatment could increase the expression of VDR, and reduce the expression of mTOR, p70s6k, 4E-BP1. The autophagy level showed that DDIT4 treatment could increase the expression of LC3I and decrease the expression of LC3II. The results of ferroptosis detection showed that DDIT4 treatment could reduce the expression of MDA and increase the expression of SOD and GSH. By participating in the VDR/mTOR/p70s6k/4E-BP1 signaling pathway, DDIT4 affects the process of autophagy and ferroptosis, thereby improving the pathological damage of diabetic kidney disease, and may become a new target for the treatment of DKD.
Confining donor conformation distributions for efficient thermally activated delayed fluorescence with fast spin-flipping
Authors: Qiu Weidong , Liu Denghui , Li Mengke , Cai Xinyi , Chen Zijian , He Yanmei , Liang Baoyan , Qiao Zhenyang , Chen Jiting , Li Wei , Xie Wentao , Peng Xiaomei , Wang Zhiheng , Li Deli , Gan Yiyang , Jiao Yihang , Pu Junrong , Gu Qing , Su Shi-Jian 
Published: November 2022

Abstract
Fast spin-flipping is the key to exploit the triplet excitons in thermally activated delayed fluorescence (TADF) based organic light-emitting diodes (OLEDs) toward high efficiency, low efficiency roll-off and long operating lifetime. In common donor-acceptor type TADF molecules, the distribution of dihedral angles in the film state would have significant influence on the photo-physical properties, which are usually neglected by researches. Herein, we found that the excited state lifetimes of TADF emitters are subjected to conformation distributions in the host-guest system. Acridine-type flexible donors have a broad conformation distribution or dual conformations, which could bring about the long-tail conformer with large singlet-triplet energy gap, resulting in long excited state lifetime. Utilization of rigid donors with steric hindrance can restrict the conformation distributions in the film to achieve degenerate singlet and triplet states, which is beneficial to efficient reverse intersystem crossing (RISC). Based on this principle, three prototype TADF emitters with confined conformation distributions were developed, achieving high RISC rate constants greater than 106 s− 1, which enable highly efficient solution-processed OLEDs with significantly suppressed efficiency roll-off.