bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recast layer removal of WEDMed surface by wire electrochemical finishing
Authors: Pan Hongwei , Liu Zhidong , Chen Xinsong , Yin Xiyuan 
Published: June 2022

Abstract
To eliminate the recast layer on the surface machined by multi-pass wire electrical discharge machining (WEDM), hence improving the surface integrity, the present paper proposed and investigated a new combined machining process of performing wire electrochemical machining (WECM) after multi-pass WEDM on the same machine tool by utilizing the slight conductivity of the compound working fluid. A theoretical model based on the electric double layer and Faraday’s law of electrolysis was established to calculate the wire feed rate for WECM finishing. Due to the exclusive film protection of the compound working fluid, the theoretical model was modified according to the experimental results under different electrolyte concentrations. Afterward, the optimized model was applied to guide the experiments of the combined process. An electrolyte concentration of 1:5 was used to facilitate the electrochemical reaction. The experimental results showed that the remaining recast layer after multi-pass WEDM could be completely removed at a wire feed rate of 10 µm/s, and a fine surface finish could be obtained. In addition, the surface roughness of the machined part was improved from 1.458 µm Ra to 0.683 µm Ra.
Bioinformatics Analysis Reveals miR-98-5p as a potential Inhibitor of Tumor cell proliferation and metastasis in Colorectal Cancer by targeting the FZD3 receptor of the Wnt signaling pathway
Authors: Kenneth Mutebi John , Haque Fahim Kabir Monjurul 
Published: June 2022

Abstract
Background
The World Health Organization (WHO) report of 2020 indicated that Colorectal Cancer (CRC) is ranked the third most common cancer type and the second cause of cancer-related deaths in the world. However, the existing treatment, as well as prognosis strategies, need to be improved to increase the survival of CRC patients. Targeted therapies of CRC as opposed to ordinary therapies; target key biological features and pathways of cancerous cells hence minimizing the subsequent damage to normal cells. MicroRNAs have been reported to play a crucial role in inhibiting and/or suppressing major pathways in various cancer types by targeting transcripts of key genes in such pathways.
Methods
This study aimed at in silico inhibiting cancer cell proliferation and metastasis by targeting a key gene – Frizzled receptor 3 (FZD3) in a major pathway of CRC called Wnt signaling; using microRNAs. The in silico analysis revealed that miR-98-5p is a direct target of FZD3, using 5 microarray datasets containing tumorous and control samples.
Results
Further analysis indicated that miR-98-5p inhibits FZD3 through binding directly to the 3’UTR of its mRNA hence exerting a suppressor role of colorectal tumors through the Wnt pathway. However, these results need to be validated in the future through basic research experiments using CRC cells in vivo and in vitro.
Conclusion
The study reveals miR-98-5p as a novel target of FZD3 and an inhibitor of the Wnt signaling pathway hence being a potential candidate for developing targeted therapies against CRC.
Influence of socioeconomic factors on PM 2.5 pattern in China’s megacities between 2013 and 2019
Authors: Rajasekar Adharsh , Ayugi Brian , Nyasulu Matthew , Babaousmail Hassen , Zhao Cailin , Gul Chaman , Wang Bangguo 
Published: June 2022

Abstract
In the last decade, Chinese megacities have undergone rapid and massive urbanisation that resulted in rising PM2.5 levels in megacities, which forced the government to implement stricter air quality guidelines to combat it. This study investigates the effectiveness of the five-year (2013–2017) air combat plan in reducing PM2.5 in these megacities since they have achieved a certain level of urbanisation. The findings show that annual concentrations of PM2.5 exceeded the World Health Organization (WHO) guideline value, and eight out of ten cities exceeded the national ambient air quality standard (NAAQS) guideline value. Although, on an annual level, a consistent downward trend was observed for PM2.5 values among all cities starting from 2013, indicating positive policy feedback from guidelines. The cumulative rate of change in PM2.5 concentrations from 2013 to 2019 indicated that the highest magnitude of the decrease occurred in Beijing and Chengdu (-59%). The Environmental Kuznets curve was observed for PM2.5 with GDP and Industrial SO2 emission. A negative relationship was observed between PM2.5 and secondary industry share, while the contrary was observed between PM2.5 and Industrial NO2 emissions. Our data indicated that stricter sanctions and emission policies are needed to lower the PM2.5 values in most of these megacities.
Secondary Psychiatric Care Patients’ Experiences of Internet CBT for Insomnia – a Qualitative Study
Authors: Sidén Frej Nicolaisen , Spak Fredrik 
Published: June 2022

Abstract
Background: Insomnia is very common, especially among psychiatric patients in secondary care. It is experienced as a 24h problem affecting several domains of life. Cognitive behavioural therapy for insomnia (CBT-I) is widely regarded as the first-line treatment and often improves comorbid disorders. Despite this, many patients are not offered CBT-I. Internet based CBT for insomnia (iCBT-I) is just as good as face-to-face treatments and could considerably increase availability. However, it is unclear whether iCBT-I is suitable for patients with more severe and comorbid psychiatric illnesses in secondary care.Methods: Eleven Swedish participants (24-68 years old) in outpatient secondary psychiatric care who underwent iCBT-I treatment were interviewed. The semi-structured interviews were analysed using content analysis. The purpose was to map their experiences, motivation and suggestions for improvement.Results: Prior to the treatment, most participants were highly motivated to take the opportunity to address their insomnia. The treatment was perceived as well-structured and interesting. The most difficult aspect was counteracting the fatigue. It was also hard to continue the treatment when faced with major life events. During this phase, contact with the therapist motivated them to continue the treatment. Several desired more face-to-face meetings. It was also motivating to gain insights into and a sense of control over sleep. Several described better sleep, improved daily routines, a more predictable everyday life and increased energy. Daytime well-being was improved in some, partly because they had more energy but also because they filled their days with more activities.Conclusions: The treatment has the potential to be very useful in secondary psychiatric care where insomnia is common and affects comorbid disorders. Psychiatric patients might have more difficulties continuing with iCBT-I treatment, but those who manage to complete the program have a good chance of obtaining benefit. Additional social support and other adjustments may enhance treatment outcomes. Participants' stories are particularly valuable, as therapists and treatment developers receive less feedback from patients in internet-based treatments compared to face-to-face treatments.
Senescence Induced by Nox4 Expressed Aberrantly in Osteoclast Precursors Accelerates Ostoeclastogenesis to Promote Osteoarthritis
Authors: Yu Bin , shen Ke , Qin Hanjun , Liu Xiaonan , Wang Lei , He Siying , Zhao Xingqi , Yang Jun , Wang Shengnan 
Published: June 2022

Abstract
Abnormal bone remodeling of subchondral bone is associated with osteoclast formation during early osteoarthritis (OA) while senescent phenotypes are detected during osteoclast differentiation. However, the associations between cellular senescence and osteoclastogenesis remain unclear in early OA. The present study found that Nox4 positively regulated the senescence of OCPs to promote multinucleated osteoclastogenesis. Overexpression of Nox4 induced overproduction of ROS, thereby promoting senescence of OCPs. Senescent OCPs secreted SASP factors to spread the senescence in OCPs clusters, over-activating the TRAF6/MAPK/c-Fos/NFATc1 axis to accelerate the fusion and osteoclast differentiation. Interestingly, the senescence of OCPs mediated by Nox4/ROS may be an essential step in initiating osteoclast differentiation. Compared to WT mice, subchondral bone loss was reduced in early OA and cartilage degeneration alleviated throughout the course of OA in Nox4-deficient mice. In addition, pharmacological inhibition of Nox4 significantly delayed OA development. In conclusion, we have identified Nox4-induced cellular senescence as an important regulator of osteoclast differentiation, suggesting that it might be a potential target to retard or prevent OA.
Identification of the unique subtype of macrophages in aneurysm lesions at the growth phase
Authors: Okada Akihiro , Koseki Hirokazu , Ono Isao , Kayahara Tomomichi , Kurita Hiroki , Miyamoto Susumu , Kataoka Hiroharu , Aoki Tomohiro 
Published: June 2022

Abstract
Background
Considered with the devastating outcome of subarachnoid hemorrhage due to rupture of intracranial aneurysm (IA), a novel therapeutic strategy based on the pathogenesis of IAs should be established. Recent experimental studies have defined IAs as a macrophage-mediated chronic inflammatory disease affecting intracranial arteries. Although there are various subtypes in macrophages, what type of macrophages is present in lesions during the disease development or contributes to the pathogenesis remains to be elucidated.
Methods
The previously-established aneurysm model of rats were used. Macrophages were labeled with the fluorescent protein, DiI, and labelled cells were isolated by a laser-microdissection method. The comprehensive gene expression profile analyses and gene ontology analyses was then done to identify a macrophage subtype present in lesions at the growth phase. The gene expression profile data from in vitro-differentiated bone marrow derived macrophages was acquired from the database and used as a reference data. The histopathological examinations to validate the results were also done.
Results
The gene expression profile data of total 52 macrophages infiltrating into the lesions was acquired. The principal component analysis failed to form multiple cluster, suggesting that macrophages infiltrating in the lesions were monotonous. By comparing the profile of the macrophage subtype identified with one from in vitro-differentiated M0 or M1 macrophages, the macrophages in the lesions were found to be belonged to the simple and unique subtype. Because the perception of signaling from nervous system was highlighted as terms up-represented compared with terms in M0 or M1 macrophages through gene ontology analyses, the macrophage subtype in the aneurysm lesions at the growth phase might be differentiated under the influence of nervous system in the microenvironment of the disease. The histopathological examinations clarified the presence of nerves in the adventitia of intracranial artery bifurcation, supporting the above notion.
Conclusions
The findings from the present study have provided the useful insights about the macrophage subtype in aneurysm lesions at the growth phase and also proposed this subtype or genes specifically expressed in this subtype as a therapeutic target.
The apical sealing effect of various on root canal fillings on isolated teeth: a systematic review
Authors: Yin Jinping , Liu Zhenglong , Liu Kehua 
Published: June 2022

Abstract
Background: Root canal filling materials that are commonly used include AH-Plus, GuttaFlow, and Resilon/Epiphany. In clinical practice, the apical closure performance of three root canal fillers is still debatable. The goal of this study was to conduct a meta-analysis to evaluate the effect of AH Plus, GuttaFlow, and Resilon/Epiphany on apical sealing in order to provide references for their clinical application.Methods: Between January 1, 2000 and June 1, 2022, the computer database was searched for randomized controlled trials on the effects of AH Plus (AH group), GuttaFlow (GF group), and Resilon/Epiphany (RE Group) root canal filling materials on apical sealing. Two evaluators independently assessed the studies' quality and extracted the data. Review 5.4 software was used to conduct a meta-analysis of the included studies.Results: A total of 10 literatures containing 452 isolated teeth were included. The meta-analysis results revealed a significant difference in apical microleakage between the RE group and the AH group [SMD = 1.45, 95% CI (1.20, 1.69), P＜0.00001] and the GF group [SMD = 1.37, 95% CI (0.80, 1.95), P＜0.00001]. There is no significant difference in apical microleakage between the AH and GF groups [SMD = 0.09, 95% CI (-0.21, 0.40), P = 0.55].Conclusions: Based on existing research findings, Resilon/Epiphany has the best apical sealing effect in the short term (≤ 1 week) when compared to AH plus and GuttaFlow.
Subjective wellbeing in developing countries: The case of rural Ethiopia
Authors: Tekleselassie Tsegay Gebrekidan 
Published: June 2022

Abstract
This study focuses on the impact of religiosity, general and political trust, local participation, and welfare metrics on wellbeing in rural areas using the Ethiopian Rural Household Survey. Ordered probit methods that account for Heteroscedasticity reveal distinctive determinants of overall life satisfaction and momentary happiness. Broader socio-economic factors such as religiosity and political governance strongly predict life satisfaction, while largely welfare metrics drive momentary happiness.
Relationship between Helicobacter pylori and idiopathic non-granulomatous anterior uveitis
Authors: Pérez-Cano Héctor Javier , Ceja-Martínez Jimena , Tellezgiron-Lara Vanessa , Voorduin-Ramos Stephanie , Morales-López Oscar , Somilleda-Ventura Selma Alin 
Published: June 2022

Abstract
Purpose: The relationship of Helicobacter pylori infection with ocular diseases, including anterior uveitis, has been reported. The objective of this study was to determine the presence of anti-H. pylori IgG antibodies in patients with idiopathic non-granulomatous anterior uveitis and compare the results with a control group. Methods: A prospective, comparative and cross-sectional study was conducted. Patients with idiopathic granulomatous anterior uveitis and a group of control subjects were included. The presence of anti-H. pylori IgG antibodies was determined. Chi square test was performed for comparative analysis with GraphPad Prism V5.0 software Results: 30 patients with idiopathic non-granulomatous anterior uveitis and 35 control subjects were included. In the determination of anti-H. pylori IgG antibodies, 24 (80%) patients and 19 (54%) control subjects were positive. A significant difference (p = 0.0263) was found between the groups and a odds ratio (OR) of 3.37. Conclusions: A direct relationship was found between the presence of anti H. pylori IgG antibodies and idiopathic non-granulomatous anterior uveitis. An association can be established between idiopathic non-granulomatous anterior uveitis and H. pylori infection, without this being a causal or physiopathogenic relationship.
DNA methylomes derived from alveolar macrophages and alveolar T cells display distinct patterns in tuberculosis – a future precision tool for TB status determination?
Authors: Pehrson Isabelle , Sayyab Shumaila , Das Jyotirmoy , Idh Nina , Paues Jakob , Méndez-Aranda Melissa , Ugarte-Gil César , Lerm Maria 
Published: June 2022

Abstract
BackgroundHost innate immune cells have been identified as key players in the early eradication of Mycobacterium tuberculosis and in the maintenance of an anti-mycobacterial immune memory, which we and others have shown are induced through epigenetic reprogramming. Studies on human tuberculosis immunity are dominated by those using peripheral blood as surrogate markers for immunity. We aimed to investigate DNA methylation patterns in immune cells of the lung compartment by obtaining induced sputum from patients with tuberculosis and subjects exposed to M. tuberculosis. Alveolar macrophages and alveolar T cells were isolated from the collected sputum and DNA methylome analyses performed (Illumina Infinium Human Methylation 450k). ResultsMultidimensional scaling analysis revealed that DNA methylomes of cells from the patients with tuberculosis, the exposed subjects and controls appeared as separate clusters. The numerous genes that were differentially methylated between the three groups were functionally connected and overlapped with previous findings of trained immunity and tuberculosis. In addition, analysis of the interferon-gamma release assay (IGRA) status of the subjects demonstrated that the IGRA status was reflected in the DNA methylome by a unique signature. Conclusions M. tuberculosis induces epigenetic reprogramming in immune cells, reflected as a specific DNA methylation pattern of immune cells in the lung compartment upon exposure to tuberculosis. The data of this pilot study suggests that DNA methylation-based biosignatures could be considered for further development towards a clinically useful tool for determining tuberculosis infection status and the level of tuberculosis exposure.
The association between Uric Acid and Diabetic Retinopathy: Review and meta-analysis
Authors: Zhang Luyuan , Li Xiaorong 
Published: June 2022

Abstract
Background
Diabetic retinopathy (DR) is a serious neuro-microvascular complication of diabetes mellitus (DM), which is due to the disturbance of metabolism. Uric acid (UA) is one of the most important indicators of the occurrence and development of metabolism disorders. Many studies reported UA in DR patients was higher than that in non-DR patients. however, the results are controversial. The purpose of this meta-analysis study was to reveal the relationship between UA level and DR.
Method
We searched the online electronic database and retrieved all the relevant articles published before March 2022. Demographic data and UA biochemical parameters in different studies were collected. The pooled standard mean deviation (SMD) and its 95% confidence interval (CI) were calculated and meta-analysis was performed.
Results
A total of 32 articles including 20658 patients were involved in this meta-analysis. The UA levels in DR group was significantly higher than that in non-DM group and NDR group (P = 0.0001; P < 0.00001, respectively). Subgroup analysis showed that there were significant differences in UA level between NPDR and PDR groups (P < 0.00001), NPDR and NDR groups (P = 0.02). In addition, UA levels were significantly higher in DR group comparing NDR group in plasma (P < 0.0001), vitreous (P < 0.00001) and aqueous humor (P < 0.0001) samples. It was also showed UA level was significantly ascended in DR patients no matterT1DM(P < 0.00001) and T2DM (P < 0.00001).
Conclusions
The UA level is definitely associated with DR severity. Therefore, increased UA concentration maybe a potential biomarker that distinguishes DR from DM, and elevated UA level implied the gradual progress of DR.
Evaluation of the marginal adaptation and gingival status of full-crown restorations using an intraoral camera
Authors: Chiu Shuting , Lee Yeh , Liu Min , Chen Hu , Ye Hongqiang , Liu Yunsong 
Published: June 2022

Abstract
Objective
The purpose of this study was to compare the usefulness of intraoral photographs, acquired with a household intraoral camera operating in conventional, calibrated, and polarized modes, with clinical examinations for assessing the marginal adaptation and gingival status of full-crown restorations.
Methods
Clinical examinations were performed by a prosthodontist who classified the marginal adaptation of full-crown restorations according to FDI World Dental Federation criteria, and a periodontal expert who classified gingival status according to the Modified Gingival Index (MGI). The margins and gingival status of the conventional, calibration, and polarization groups of full-crown restorations were independently assessed by three evaluators who obtained photographs using an intraoral camera. Cases where at least two of three assessors were in agreement were analyzed using Cohen’s kappa coefficient and the chi-square test, and the sensitivity and specificity were calculated.
Results
The conventional, calibration, and polarization groups differed significantly in marginal and gingival status of full-crown restorations. In the calibration group, there was good agreement between the camera-based and oral clinical examinations in terms of the gingival status of full-crown restorations (kappa = 0.945), with 100% sensitivity and 91.67% specificity; this was also the case in the polarization group with respect to the margins of full-crown restorations (kappa = 0.917, sensitivity = 97.22%, specificity = 94.44%).
Conclusions
An intraoral camera with black and white calibrated images is useful to assess the gingival status of full-crown restorations. Polarization mode can be used to assess the marginal adaptation of full-crown restorations. The camera is a feasible and valid diagnostic aid.
Partial replacement of fishmeal by Azolla cristata in diets for fingerling common carp, Cyprinus carpio var. communis
Authors: Ahmed Imtiaz , Khan Younis Mohd , Lateef Anzar , Majeed Aamir , Shah Manzoor A. 
Published: June 2022

Abstract
A 12-week growth experiment was conducted to evaluate the suitability of Azolla cristata as fish meal substitute for fingerling Cyprinus carpio var Communis. Six iso-nitrogenous and isocaloric (16.17 kJ g− 1 GE) diets were formulated to contain 42% crude protein and each treatment had three replicates with a mean initial weight of 3.4 ± 0.2 g. A. cristata in gradation of 0, 10, 20, 30, 40 and 50% were fed in order to check possible replacement of fish meal. No significant (P > 0.05) differences in growth performance of fish fed on diets containing up to 10% inclusion and the control were seen. However, further increase in Azolla meal resulted in progressively reduced growth performance of fish in terms of live weight gain (LWG), specific growth rate (SGR), feed conversion ratio (FCR) and protein retention efficiency (PRE). All hematological parameters had a linear declining trend as the proportion of Azolla meal in the diet was increased. Protein content was found significantly (P < 0.05) reduced in the fish fed higher inclusion of Azolla meal. Serum biochemical parameters were also found to reduce with increasing inclusion of Azolla meal, except for glucose, cholesterol, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) which showed higher concentration with increasing inclusion of Azolla meal. Based on the above results, it is suggested that 10% Azolla meal can be added as a replacement of fishmeal in practical diets for C. carpio communis, which would be helpful in reducing the cost of the feed as well as the appropriate use of aquatic macrophytes.