bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short Chain Fatty Acids Taken at Time of Thrombectomy in Acute Ischemic Stroke Patients Are Independent of Stroke Severity But Associated With Inflammatory Markers and Worse Symptoms at Discharge
Authors: Henry Nicholas , Frank Jacqueline , McLouth Christopher , Trout Amanda L. , Morris Andrew , Chen Jianzhong , Stowe Ann M. , Fraser Justin F. , Pennypacker Keith 
Published: January 2022

IntroductionShort chain fatty acids (SCFA) are gut microbiota-derived metabolites that contribute to the gut-brain axis and may impact stroke outcomes following gut dysbiosis. We evaluated plasma SCFA concentrations against stroke severity parameters and identified SCFA-associated protein networks.MethodsThe Blood and Clot Thrombectomy Registry and Collaboration (BACTRAC), a continuously enrolling tissue bank, was used to obtain stroke samples. Arterial blood distal and proximal to the thrombus was obtained from Acute Ischemic Stroke (AIS) Patients (n=53) during thrombectomy. Patient demographics, stroke presentation and outcome parameters were reported. The SCFAs were isolated from proximal plasma via chemical derivatization UHPLC coupled tandem mass spectrometry using electrospray ionization and multiple reaction monitoring. Proteomic levels for 184 cardioembolic and inflammatory proteins was quantified from systemic and intracranial plasma by Olink. Arterial blood from cerebrovascular patients undergoing elective neurointerventional procedures was used as controls.ResultsAcetate positively correlated with time from last known normal (LKN) and was significantly lower in stroke patients compared to control. Isobutyrate, Butyrate and 2-Methylbutyrate negatively correlated with %ΔNIHSS. Isobutyrate and 2-Methylbutyrate positively correlated with NIHSS discharge. SCFA concentrations were not associated with NIHSS admission, infarct volume, or edema volume. Multiple SCFAs positively associated with systemic and pro-inflammatory cytokines, most notably IL-6, TNF-α, VCAM1, IL-17, and MCP-1.ConclusionsPlasma SCFA concentrations taken at time of stroke are not associated with stroke severity at presentation. However, higher levels of SCFAs at the time of stroke are associated with increased markers of inflammation, less recovery from admission to discharge, and worse symptom burden at discharge.
The Immune Response to SARS-CoV-2 Vaccination: Insights Learned From Adult Patients With Common Variable Immune Deficiency
Authors: Quinti Isabella , Locatelli Franco , Carsetti Rita 
Published: January 2022

CVID patients have an increased susceptibility to vaccine-preventable infections. The question on the potential benefits of immunization of CVID patients against SARS-CoV-2 offered the possibility to analyze the defective mechanisms of immune responses to a novel antigen. In CVID, as in immunocompetent subjects, the role of B and T cells is different between infected and vaccinated individuals. Upon vaccination, variable anti-Spike IgG responses have been found in different CVID cohorts. Immunization with two doses of mRNA vaccine did not generate Spike-specific classical memory B cells (MBCs) but atypical memory B cells (ATM) with low binding capacity to Spike protein. Spike-specific T-cells responses were also induced in CVID patients with a variable frequency, differently from specific T cells produced after multiple exposures to viral antigens following influenza virus immunization and infection. The immune response elicited by SARS-CoV-2 infection was enhanced by subsequent immunization underlying the need to immunize convalescent COVID-19 CVID patients after recovery. In particular, immunization after SARS-Cov-2 infection generated Spike-specific classical memory B cells (MBCs) with low binding capacity to Spike protein and Spike-specific antibodies in a high percentage of CVID patients. The search for a strategy to elicit an adequate immune response post-vaccination in CVID patients is necessary. Since reinfection with SARS-CoV-2 has been documented, at present SARS-CoV-2 positive CVID patients might benefit from new preventing strategy based on administration of anti-SARS-CoV-2 monoclonal antibodies.
Physicochemical Characteristics of Aedes Mosquito Breeding Habitats in Suburban and Urban Areas of Kinshasa, Democratic Republic of the Congo
Authors: Mbanzulu Kennedy Makola , Mboera Leonard E. G. , Wumba Roger , Engbu Danoff , Bojabwa Michael Mondjo , Zanga Josué , Mitashi Patrick Mulopo , Misinzo Gerald , Kimera Sharadhuli Iddi 
Published: January 2022

BackgroundThe knowledge of key elements of the ecosystem affecting mosquito distribution and their population dynamics is essential for designing mosquito-borne disease interventions. The present study characterized the physicochemical properties ofAedesmosquito breeding habitats in Democratic Republic of the Congo.MethodsA cross-sectional survey was carried out in Kinshasa, from February to April 2021. The physicochemical characteristics of the natural and artificial aquatic habitats ofAedeswere measured using a multiparametric device.ResultsOut of 438 breeding habitats inspected, 273 (62.3%) contained mosquito larvae. TheAedesmosquitoes identified in 76.19% of positive breeding sites wereAedes albopictus(67.30%) andAedes aegypti(37.98%). The median values of dissolved oxygen (DO) (1.0), turbidity (19.15), and salinity (0.115) in water breeding sites ofAedeswere respectively 0.8, 55.0, and 0.29 inCulexbreeding sites (p&lt; 0.05). The physicochemical characteristics of the breeding habitat forAe. aegyptiandAe. albopictuswere almost identical. In urban areas, the median temperature was 29.82 while it was 29.60 in suburban areas (p&lt; 0.05). Significantly, the salinity was higher in bamboo and metal containers while DO was higher in tins. After analysis using simple linear regression, total dissolved solids (r = 0.23;p= 0.000), conductivity (r = 0.23), salinity (r = 0.23), and temperature (r = 0.13) were associated with larval density (p&lt; 0.05). In the final model (r = 0.30,p= 0.01), salinity (r = 0.23) and DO (r = 0.138) adjusted to temperature, pH, and turbidity were associated positively to larvae density.ConclusionTheAedesbreeding sites and mosquito density were significantly influenced by water salinity, DO, temperature, pH, and turbidity.
Characterization of Heavily Irradiated Dielectrics for Pixel Sensors Coupling Insulator Applications
Authors: Bharthuar S. , Golovleva M. , Bezak M. , Brücken E. , Gädda A. , Härkönen J. , Karadzhinova-Ferrer A. , Kramarenko N. , Kirschenmann S. , Koponen P. , Luukka P. , Mizohata K. , Ott J. , Tuominen E. 
Published: January 2022

An increase in the radiation levels during the high-luminosity operation of the Large Hadron Collider calls for the development of silicon-based pixel detectors that are used for particle tracking and vertex reconstruction. Unlike the conventionally used conductively coupled (DC-coupled) detectors that are prone to an increment in leakage currents due to radiation, capacitively coupled (AC-coupled) detectors are anticipated to be in operation in future collider experiments suitable for tracking purposes. The implementation of AC-coupling to micro-scale pixel sensor areas enables one to provide an enhanced isolation of radiation-induced leakage currents. The motivation of this study is the development of new generation capacitively coupled (AC-coupled) pixel sensors with coupling insulators having good dielectric strength and radiation hardness simultaneously. The AC-coupling insulator thin films were aluminum oxide (Al2O3) and hafnium oxide (HfO2) grown by the atomic layer deposition (ALD) method. A comparison study was performed based on the dielectric material used in MOS, MOSFET, and AC-coupled pixel prototypes processed on high resistivity p-type Magnetic Czochralski silicon (MCz-Si) substrates. Post-irradiation studies with 10 MeV protons up to a fluence of 1015 protons/cm2 suggest HfO2 to be a better candidate as it provides higher sensitivity with negative charge accumulation on irradiation. Furthermore, even though the nature of the dielectric does not affect the electric field within the AC-coupled pixel sensor, samples with HfO2 are comparatively less susceptible to undergo an early breakdown due to irradiation. Edge-transient current technique (e-TCT) measurements show a prominent double-junction effect as expected in heavily irradiated p-type detectors, in accordance with the simulation studies.
CBCT Verification of SRT for Patients With Brain Metastases
Authors: Papp Judit , Simon Mihály , Csiki Emese , Kovács Árpád 
Published: January 2022

BackgroundThe aim of our work is to demonstrate the role of image guidance and volumetric imaging in stereotactic radiotherapy (SRT) of brain metastases.MethodsBetween 2018 and 2020, 106 patients underwent intracranial stereotactic radiotherapy. 10 patients with metastatic brain tumors treated with SRT were randomly selected and included in our study model. Patients were scanned pre- and post-treatment with cone beam CT. Total of 100 verifications of 50 stereotaxic treatments were performed and analyzed.ResultsPopulation mean X, Y, Z values were -0.13 cm, -0.04 cm, -0.03 cm, respectively, rotation values 0.81°, 0.51°, 0.46°, respectively. Systematic error components for translational displacements pre corrections were as follows: 0.14 cm for X, 0.13 cm for Y and 0.1 cm for Z. Systematic error components of the post-treatment HR 3D CBCTs were as follows: 0.01 cm for X, 0.06 cm for Y and 0.04 cm for Z.ConclusionsPopulation mean values close to 0 confirmed that there is no systematic variation in our system and the accuracy of our equipment and tools is reliable. HR 3D CBCT scans performed pre SRTs further refine patient and target volume setting, support medical decision making and eliminate the possibility of gross error.
Cutaneous Toxicity Associated With Enfortumab Vedotin: A Real-Word Study Leveraging U.S. Food and Drug Administration Adverse Event Reporting System
Authors: Yang Hui , Yu Xiaojia , An Zhuoling 
Published: January 2022

IntroductionEnfortumab vedotin (EV) has been demonstrated to have a significant response rate in early phase trials and is known for its tolerable side-effect profile. Emerging case reports have raised awareness of cutaneous toxicities, which may be a potentially fatal complication.ObjectiveTo assess the potential relevance between EV and cutaneous toxicities reports through data mining of the U.S. Food and Drug Administration (FDA) adverse event reporting system (FAERS).MethodsData from January 1, 2019, to November 4, 2021, in the FAERS database were retrieved. Information component (IC) and reporting odds ratio (ROR) were used to evaluate the association between EV and cutaneous toxicities events.ResultsEV was significantly associated with cutaneous toxicities in the database compared with both all other drugs (ROR 12.90 [10.62–15.66], IC 2.76 [2.52–3.01], middle signal) and platinum-based therapy (ROR 15.11 [12.43–18.37], IC 2.91 [2.66–3.15], middle signal) in the FAERS database. A significant association was detected between EV and all the cutaneous adverse effects (AEs) except erythema, palmar–plantar erythrodysesthesia syndrome, and dermatitis allergic. Both Stevens–Johnson syndrome and toxic epidermal necrolysis occurred 15 times as frequently for EV compared with all other drugs (ROR = 15.20; ROR = 15.52), while Stevens–Johnson syndrome occurred 18 times and toxic epidermal necrolysis occurred 7 times as frequently for EV compared with platinum-based therapy in the database (ROR = 18.74; ROR = 7.80). All groups that limited the gender and age showed a significant association between EV and cutaneous toxicities.ConclusionsA significant signal was detected between EV use and cutaneous toxicities. It is worth noting that Stevens–Johnson syndrome and toxic epidermal necrolysis were significantly associated with EV use.
The Gene Expression Analysis of Peripheral Blood Monocytes From Psoriasis Vulgaris Patients With Different Traditional Chinese Medicine Syndromes
Authors: Lu Yue , Qi Yao , Li Li , Yan Yuhong , Wei Jianan , Yao Danni , Wu Jingjing , Deng Hao , Deng Jingwen , Ye Shuyan , Chen Haiming , Chen Qubo , Gao Hengjun , Han Ling , Lu Chuanjian 
Published: January 2022

Psoriasis is chronic skin disease and an important health concern. Traditional Chinese Medicine (TCM) has shown great promise in the treatment of psoriasis. However, the correlation between TCM Syndromes and genomics of psoriasis has not been evaluated. Here, we analyzed gene expression profiling of monocytes from psoriasis vulgaris patients with different TCM syndrome types to reveal the molecular basis of different psoriasis syndromes. Of the 62 cases of psoriasis vulgaris recruited, 16, 23, and 23 cases were of blood-heat syndrome, blood stasis syndrome, and blood-dryness syndrome, respectively; 10 healthy controls were recruited as controls. Affymertix’s Gene Chip ®clariom D gene chip was used to detect the gene expression profile of peripheral blood monocytes collected from recruited individuals. Compared with the healthy control group, 1570 genes were up-regulated and 977 genes were down-regulated in the psoriasis vulgaris patients group; 798 genes and 108 genes were up- and down-regulated in the blood-heat syndrome group respectively; 319 and 433 genes were up- and down-regulated in the blood-dryness syndrome group, respectively; and 502 and 179 genes were up-and down-regulated in the blood-stasis syndrome group. Our analyses indicated not only common differential genes and pathways between psoriasis syndrome groups and healthy controls, but also syndrome-specific genes and pathways. The results of this study link the three syndromes at the gene level and will be useful for clarifying the molecular basis of TCM syndromes of psoriasis.Clinical Trial Registration: (http://www.chictr.org.cn/showproj.aspx?proj=4390), identifier (ChiCTR-TRC-14005185).
Virtual Reality and EEG-Based Intelligent Agent in Older Adults With Subjective Cognitive Decline: A Feasibility Study for Effects on Emotion and Cognition
Authors: Cuesta Marc , Verty Lynn Valeyry , Ben Abdessalem Hamdi , Byrns Alexie , Bruneau Marie-Andrée , Frasson Claude , Belleville Sylvie 
Published: January 2022

Objectives: Immersive virtual reality has tremendous potential to improve cognition in populations with cognitive impairment. We conducted a feasibility and proof-of-concept study to assess the potential of virtual reality and electroencephalography, with or without an intelligent agent, that adapts the presented material to the emotions elicited by the environment.Method: Older adults with subjective cognitive decline recruited from the community received a virtual reality-based intervention taking place in one of two virtual environments, a train (Part 1, N = 19) or a music theatre, complemented by the intelligent agent (Part 2, N = 19). A comparative control group (N = 19) receiving no intervention was also included. All participants completed measures of affect and cognition before and after the intervention. The intervention groups completed measures of cybersickness and user experience after the intervention.Results: Participants did not suffer from increased cybersickness following either intervention. They also reported a positive to highly positive user experience concerning the following aspects: attractivity, hedonic quality-identity and hedonic quality-stimulation. The measures of affect showed no pre-post change when comparing either intervention to the control condition. However, a reduction of negative affect was observed following the train intervention for participants with a high self-reported negative affect at baseline. Finally, there was a significant improvement in working memory when comparing either intervention group to the control condition.Conclusion: Our results support the feasibility and tolerability of the technology, and a positive impact on cognition, paving the way for a larger-scale randomized clinical trial to confirm efficacy.
Portable vs. Benchtop NIR-Sensor Technology for Classification and Quality Evaluation of Black Truffle
Authors: Kappacher Christoph , Trübenbacher Benedikt , Losso Klemens , Rainer Matthias , Bonn Günther K. , Huck Christian W. 
Published: January 2022

Truffles represent the best known and most expensive edible mushroom. Known as Ascomycetes, they belong to the genus Tuber and live in symbiosis with plant host roots. Due to their extraordinary taste and smell, truffles are sold worldwide for high prices of up to 3000–5000 euros per kilogram (Tuber magnatum PICO). Amongst black truffles, the species Tuber melanosporum VITTAD. is highly regarded for its organoleptic properties. Nonetheless, numerous different sorts of black truffle are offered at lower prices, including Tuber aestivum VITTAD., Tuber indicum and Tuber uncinatum, which represent the most frequently consumed types. Because truffles do not differ visually for inexperienced consumers, food fraud is likely to occur. In particular, for the highly prized Tuber melanosporum, which morphologically forms very similar fruiting bodies to those of Tuber indicum, there is a risk of fraud via imported truffles from Asia. In this study, 126 truffle samples belonging to the four mentioned species were investigated by four different NIR instruments, including three miniaturized devices—the Tellspec Enterprise Sensor, the VIAVI solutions MicroNIR 1700 and the Consumer Physics SCiO—working on different technical principles. Three different types of measurement techniques were applied for all instruments (outer shell, rotational device and fruiting body) in order to identify the best results for classification and quality assurance in a non-destructive manner. Results provided differentiation with an accuracy up to 100% for the expensive Tuber melanosporum from Tuber indicum. Classification between Tuber melanosporum, Tuber indicum, Tuber aestivum and Tuber uncinatum could also be achieved with success of 100%. In addition, quality monitoring including discrimination between fresh and frozen/thawed, and prediction of the approximate date of harvesting, was performed. Furthermore, feasibility studies according to the geographical origin of the truffle were attempted. The presented work compares the performance for prediction and quality monitoring of portable vs. benchtop NIR devices and applied measurement techniques in order to be able to present a suitable, accurate, fast, non-destructive and reliable method for consumers.
‘You know where we are if you need us.’ The role of primary care in supporting patients following pancreaticoduodenectomy for cancer: a qualitative study
Authors: Taylor Anna Kathryn , Kausar Ambareen , Chang David , Phelan Alison , Chew-Graham Carolyn Anne 
Published: January 2022

BackgroundTen per cent of patients diagnosed with pancreatic cancer undergo pancreaticoduodenectomy. It is known that these patients have unmet psychological support needs, and GPs are key in enabling effective coordination of care for people living with life-shortening conditions.AimTo explore patients’ perspectives on the role of primary care in their management, and their sources of support.Design & settingInductive qualitative study of patients who had undergone pancreaticoduodenectomy between 6 months and 6 years previously for pancreatic or distal biliary duct cancers. Participants were recruited by clinical nurse specialists (CNSs) from a single NHS trust in Northwest England.MethodSemi-structured interviews, either face-to-face or via video link, were conducted with 20 participants. Interviews were audio-recorded, transcribed, and anonymised. Thematic analysis utilised principles of constant comparison.ResultsParticipants described immense treatment burden and uncertainty around the role of the GP in their ongoing care. They recognised that GPs may have little experience of patients who have undergone pancreaticoduodenectomy, but felt that GPs can play a vital role in offering support. Participants wished for emotional support postoperatively, and valued support networks including family and friends. However, they found expressing their deepest fears difficult. Participants felt they would value greater recognition by primary care of both physical and psychological sequelae of major pancreatic surgery, and the impact on their families.ConclusionPatients may feel themselves to be a ‘burden’ to both healthcare professionals and their own support networks following pancreaticoduodenectomy. Primary care is in a key position to proactively offer psychological support.
Blood Flow Regulates Glomerular Capillary Formation in Zebrafish Pronephros
Authors: Nishimura Yusuke , Ishii Tomohiro , Ando Koji , Yuge Shinya , Nakajima Hiroyuki , Zhou Weibin , Mochizuki Naoki , Fukuhara Shigetomo 
Published: January 2022

Background
The renal glomerulus is a tuft of capillaries in Bowman’s capsule and functions as a blood-filtration unit in the kidney. The unique glomerular capillary tuft structure is relatively conserved through vertebrate species. However, the morphogenetic mechanism governing glomerular capillary tuft formation remains elusive.

Methods
To clarify how glomerular capillaries develop, we analyzed glomerular capillary formation in the zebrafish pronephros by exploiting fluorescence-based bio-imaging technology.

Results
During glomerular capillary formation in the zebrafish pronephros, endothelial cells initially sprouted from the dorsal aorta and formed the capillaries surrounding the bilateral glomerular primordia in response to podocyte progenitor-derived vascular endothelial growth factor-A. After formation, blood flow immediately occurred in the glomerular primordia-associated capillaries, while in the absence of blood flow, they were transformed into sheet-like structures enveloping the glomerular primordia. Subsequently, blood flow induced formation of Bowman’s space at the lateral sides of the bilateral glomerular primordia. Concomitantly, podocyte progenitors enveloped their surrounding capillaries while moving toward and coalescing at the midline. These capillaries then underwent extensive expansion and remodeling to establish a functional glomerular capillary tuft. However, stopping blood flow inhibited the remodeling of bilateral glomerular primordia, which therefore remained unvascularized but covered by the vascular sheets.

Conclusions
We delineated the morphogenetic processes governing glomerular capillary tuft formation in the zebrafish pronephros and demonstrated crucial roles of blood flow in its formation. Blood flow maintains tubular structures of the capillaries surrounding the glomerular primordia and promotes glomerular incorporation of these vessels by inducing the remodeling of glomerular primordia.
UMA LEITURA FOUCAULTIANA DAS CARTAS DE SÊNECA: CUIDADO DE SI, ESCRITA DE SI, LEITURA DE SI
Authors: Pereira David Da Silva , Pereira Silvana Dias Cardoso 
Published: January 2022

Michel Foucault ensinou no Collège de France entre 02.dez.1970 e 25.jun.1984, de sua aula inaugural ao seu desaparecimento. Nesse último ano, profere o Curso à "A Coragem da Verdade", por meio do qual, compartilha uma certa forma de interpretação das Cartas de Sêneca no âmbito de uma investigação sobre a aleturgia - processos de produção do dizer verdadeiro. Esta comunicação estende-se ainda um artigo publicado em 1983, em uma série de estudos e conferências sobre as artes de si, para restituir elementos de um conjuntos de atos de cuidado, de escrita, de leitura de si, a partir de um trabalho realizado estritamente sobre esses escritos e sobre o que eles deixaram de contribuição para o pensamento no presente. Nessas conferências, meses antes, Foucault já anunciara, de modo específico em sua quarta Conferência, aspectos fundamentais dessas práticas estoicas em um contexto de aprofundamento de uma investigação acerca do Cuidado de Si e da Parresía em seus três últimos Cursos do Collége. Evidenciam-se os efeitos desses exercícios na transformação do sujeito, na autoconstituição do sujeito, na transformação de si por meio da relação com o outro a partir dos elementos oferecidos pela compreensão foucaultiana. Palavras-chave: Cuidado de si; Escrita de si; Leitura de Si.