bluuebunny/crossref_metadata_2025_split huggingface dataset:
INFLUÊNCIA DA RESERVA VENTILATÓRIA NA MOBILIDADE DIAFRAGMÁTICA EM PACIENTES COM DOENÇA PULMONAR OBSTRUTIVA CRÔNICA
Authors: Oliveira Flávia Roberta Rocha de , Gonçalez Jéssica Canizelli , Brüggemann Ana Karla Vieira , Gonçalves Márcia Aparecida , Leal Bruna da Cunha Estima , Francisco Davi de Souza , Yamaguti Wellington Pereira dos Santos , Paulin Elaine 
Published: July 2022

Objetivo: Investigar a influência da reserva ventilatória (RV) na mobilidade diafragmática (MD) em pacientes com doença pulmonar obstrutiva crônica (DPOC), bem como comparar as características antropométricas, função pulmonar, MD, dispneia e capacidade de exercício entre os grupos RV >11litros/minuto (l/min) e RV <11l/min. Métodos: Tratou-se de um estudo transversal, no qual foram avaliados 42 pacientes com DPOC. No primeiro dia os pacientes realizaram espirometria e avaliação da dispneia. No segundo dia fizeram o teste da caminhada de seis minutos, e após uma semana foi avaliada a MD. A amostra foi subdividida nos grupos RV >11litros/minuto (l/min) e RV <11l/min para comparação entre os grupos. Resultados: A RV influenciou em 25% a MD (p=0,001, F=13,78). Ao comparar os grupos RV ? 11l/min e RV ? 11l/min, constatou-se que no grupo RV ?11l/min os pacientes apresentaram redução das variáveis: capacidade inspiratória (CI), volume expiratório forçado no primeiro segundo (VEF1), MD, pior capacidade submáxima de exercício e maior dispneia. Conclusões: A RV influencia a MD. Além disso, o grupo RV <11l/min foi mais comprometido tanto na função pulmonar e MD quanto na capacidade submáxima de exercício e dispneia.
DESCRIBING DIFFICULT SHELL-HASH ASSEMBLAGES FROM THE LOWER CAMBRIAN SOLTANIEH FORMATION, ALBORZ MOUNTAINS, NORTHERN IRAN
Authors: LINDSAY-KAUFMAN AMELIA , ROSBACH STEPHANIE A. , WRIGHT LAUREN S. , EDWARDS EMILY L.V. , VAZIRI SEYED HAMID , MAJIDIFARD MAHMOUD REZA , SELLY TARA , LAFLAMME MARC , SCHIFFBAUER JAMES D. 
Published: July 2022

ABSTRACTThe fossil record spanning the latest Ediacaran and earliest Cambrian is characterized by the proliferation of small, mineralized organisms that comprise the well-known and abundant deposits of small shelly fauna. Many of these fossils are tubular or conical forms with simple morphologies, and thus present difficulties in both taxonomic and phylogenetic interpretation. This study investigates a community of poorly preserved shelly tubicolous organisms in two fossiliferous slabs from the Soltanieh Formation, northern Iran. Analysis of the taphonomy of this fossil assemblage using thin-section petrography, scanning electron microscopy, and energy dispersive X-ray spectroscopy, suggests a two-part preservational pathway involving phosphatic replacement of the shell wall and separate, diagenetically later infillings of void space with either phosphatic or calcium carbonate cements. In parallel with the taphonomic study and given the difficulty in assigning the observed fossils taxonomically, morphometrics of the shelly organisms were also explored. Biometric measurements were collected from high-resolution photomosaic images of the slab-surface fossils, as well as from a three-dimensional volume of the interior of one of the slabs generated via X-ray tomographic microscopy. Statistical analysis of these measurements revealed a separation of the fossils into two morphologically distinct groups of conical and tubular forms, which we characterize respectively as ‘conomorphs' and ‘tubomorphs'. Based on previous studies of fossils from the Soltanieh Fm., we can offer tentative generic-level assignment to Anabarites and Cambrotubulus to at least some of the fossils present, though these are dependent on views in thin section rather than morphometric distinction. Cumulatively, we provide a conservative, taxonomy-free approach for detailing the morphology and preservation of poorly preserved fossils from the Ediacaran–Cambrian transition.
A 3-year retrospective study of 866 children and adolescent outpatients followed in the Nice Pediatric Psychotrauma Center created after the 2016 mass terror attack.
Authors: Gindt Morgane , Fernandez Arnaud , Zeghari Radia , Ménard Marie-Line , Nachon Ophélie , Richez Aurélien , Auby Philippe , Battista Michèle , Askenazy Florence 
Published: July 2022

Abstract
Background
The mass terrorist attack in Nice, France, in July 2016 caused deaths and injuries in a local population, including children and adolescents. The Nice Pediatric Psychotrauma Center (NPPC) was opened to provide mental health care to the pediatric population (0–18 years) who experienced traumatic events.
Objectives
To report clinical findings and provide considerations focusing on 1) Traumatic events; 2) Psychiatric diagnoses; 3) Needed care of pediatric population; 4) Recommendations based on the experience of NPPC
Methods
In this retrospective study, we conducted quantitative and qualitative data collection and analysis of new and follow-up consultations, primary and comorbid diagnoses, and the kind of trauma (terrorist attack versus other kinds of trauma). Ethics approval was obtained from the local Ethics committee.
Results
866 children and adolescents were followed in the NPPC. A high number of children and adolescents impacted by the terrorist attack required follow-up consultations (average of 30 consultations per week). After exposure to the mass terrorist attack, the first care-seeking requests continued to occur three years later, although at a slower rate than in the first and second years. New consultations for other kinds of trauma were observed over time. We found a high rate of Post-Traumatic Stress Disorder (PTSD; 71%) in this population with a high rate of comorbidities (67%), mainly sleep disorders (34.7%) and mood and anxiety disorders (16.2%).
Discussion
This study supports previous findings on the significant impact of mass trauma in the pediatric population showing even a higher level of PTSD and a high rate of comorbidities. This may be explained by the brutality of the traumatic event, particularly for this age group. The findings of this study have implications for early interventions and long-term care for children and adolescents to prevent the development of chronic PTSD into adulthood.
Hypoxia-induced reprogramming of glucose-dependent metabolic pathways maintains the stemness of human bone marrow-derived endothelial progenitor cells
Authors: Lin Dongni , Yan Kaihao , Chen Lingyun , Chen Junxiong , Xu Jianing , Xie Zhijing , Li Zhujun , Lin Shuo , Li Jinghuan , Chen Zhenzhou 
Published: July 2022

Abstract
Background aimsThe benefits of hypoxia for maintaining the stemness of cultured human bone marrow-derived endothelial progenitor cells (BM EPCs) have previously been demonstrated but the mechanisms responsible remain unclear. There is growing evidence to suggest a role for cellular metabolism in the regulation of stem cell fate and self-renewal. This study aimed at exploring changes in glucose metabolism and roles in maintaining BM EPCs stemness under hypoxia.MethodsExtracellular flux analysis, LC-MS/MS and 13C tracing HPLC-QE-MS were used to establish EPC metabolic status. Then inhibitors of glucose metabolism were used to assess the impact of the dependent pathways on cell stemness. The key enzymes of glycolysis, tricarboxylic acid cycle (TCA), pentose phosphate pathway (PPP) and mitochondrial respiration were inhibited, and the cell survival rate, clone formation rate, mRNA expression of stemness markers, Nanog, Oct4, Klf4 and Sox2, and adenosine triphosphate (ATP) level were compared.ResultsReprogramming of pathways concerned with glucose metabolism was found under hypoxic conditions, including increased rates of flux through glycolysis and the pentose phosphate pathway (PPP), together with decreased flux through the tricarboxylic acid (TCA) cycle and mitochondrial respiration. We found that inhibiting glycolysis or PPP impaired cell proliferation either under normoxia or hypoxia. Moreover, promoting pyruvate oxidation reverses the maintenance effect of hypoxia on cell stemness. On the contrary, inhibiting pyruvate oxidation, TCA or ETC increased cell stemness under normoxia mimicking hypoxic conditions. Although hypoxia decreased mitochondrial ATP level, the total ATP level remained unchanging, indicating that energy production does not play a major role in this process. ConclusionIn summary, hypoxia induced glucose metabolic reprogramming, and such pattern of metabolic reprogramming maintains the stemness of BM EPCs, and artificial manipulation of cell metabolism can be an effective way for regulating the stemness of BM EPCs.
Prevalence of depression and its correlation with anxiety, headache and sleep disorders amongst medical staff in South China
Authors: Lu Guangshuang , Xiao Shaobo , He Jiaji , Xie Wei , Ge Wei , Meng Fanchao , Yang Ying , Yu Shengyuan , Liu Ruozhuo 
Published: July 2022

Abstract
Background
The aim of this cross-sectional survey was to investigate the prevalence of depression in medical staff and its risk factors, as well as the association between depression, anxiety, headache, and sleep disorders.
Methods
Stratified random cluster sampling was used to select medical staff from various departments in four hospitals in Sanya City. The Self-rating Depression Scale(SDS), Self-rating Anxiety Scale (SAS) and Pittsburgh Sleep Quality Index (PSQI) were used to quantitatively score the depression, anxiety and sleep disorders. Correlation analysis and regression analysis were performed on the factors affecting the occurrence and severity of depression.
Results
Among 645 medical staff, 548 (85%) responded. The 1-year prevalence of depression was 42.7% and the prevalence of depression combined with anxiety, headache and sleep disorders was 23%, 27% and 34.5%. The prevalence of depression in females, nurses, unmarried or single group, and rotating-shift population was significantly higher than that in males(48.3% vs 27.1%, OR = 2.512), doctors(55.2% vs 26.7%, OR = 3.388), married group(50.5% vs 35.8%, OR = 1.900), and day-shift population(35.2% vs 7.5%, OR = 1.719). The occurrence of depression was correlated with anxiety, sleep disorder, headache and migraine, with anxiety being the most significant (Spearman Rho = 0.531). The SDS was significantly correlated with SAS and PSQI (Spearman Rho = 0.801, 0.503), and also related to the presence of headache and migraine (Spearman Rho = 0.228, 0.159). Multivariate logistic regression indicated that nurse occupation and anxiety were risk factors for depression, while grades of anxiety, sleep disorders and nurse occupation were risk factors for depression degree in multiple linear regression.
Conclusion
The prevalence of depression in medical staff is higher than that in the general population especially among females, nurse occupation, unmarried people and rotating-shift population. Depression is associated with anxiety, sleep disorders, headache and migraine. Anxiety and nurse occupation are risk factors for depression. This study might provide a reference for the promotion of occupational health among medical staff.
WITHDRAWN: Design and application of non-circular gear with cusp pitch curve
Authors: Liu JianGang , Tong ZhiPeng , Yu Gaohong , Zhao Xiong , Zhou HaiLi 
Published: July 2022

Abstract
To solve the design problem of non-circular gear with cusp pitch curve, this paper proposed a new involute and incomplete variable cycloid composite tooth profile, deduced the new involute and incomplete variable cycloid composite profile mathematical model, and constructed the conjugate gear model based on the envelope method. The design software of non-circular gear with cusp pitch curve was developed based on MATLAB. The variation law of rolling radius on incomplete cycloid profile and its characteristics such as pressure angle and radius of curvature were analyzed. The variation relationship of rolling radius on involute and incomplete cycloid composite profile meshing line and contact ratio were studied. The variation relationship of incomplete cycloid profile shape, pressure angle, curvature radius corresponding to different elliptical eccentricity were analyzed. The meshing analysis of the non-circular gear transmission mechanism was carried out based on virtual software. Comparison of the consistency of theoretical value, simulation value of the transmission ratio curve verified that the tooth profile design method was feasible, and the composite tooth profile was applied to the seedling pick-up mechanism of the non-circular gear planetary gear train. Through the seedling picking experiment of the seedling pick-up mechanism, the feasibility of the application of the involute and incomplete variable cycloid composite tooth profile was verified.
Exploration of natural product database for the identification of potent inhibitor against IDH2 mutational variants for glioma therapy
Authors: Murali Poornimaa , Karuppasamy Ramanathan 
Published: July 2022

Abstract
Mutation in Isocitrate dehydrogenase 2 (mIDH2) is an oncogenic driver prevalently reported in various cancer types including gliomas. To date, enasidenib is the only FDA-approved drug widely used as a mIDH2 (R140Q) inhibitor. However, dose-limiting toxicity and modest brain penetrating capability restrict its use as a plausible mIDH2 inhibitor. Furthermore, secondary site mutations (Q316E and I319M) were identified in patients with enasidenib treatments resulting in acquired therapeutic resistance. Hence, in the present investigation, we aimed to identify novel and potent drug-like compounds to overcome the existing drawbacks using an integrated in-silico strategy. A sum of 1574 natural compounds from the Naturally occurring Plant-based Anti-cancerous Compound Activity Target (NPACT) database was proclaimed and subjected to screening processes such as similarity calculations and molecular docking. The binding affinities of the resultant natural compounds were rescored using MM-GBSA and RF scoring functions. The toxicity and drug likeliness of the lead compounds were investigated using ADMET properties. Eventually, the integrated in-silico approach resulted in a lead molecule namely squalene (NPACT00954) against mIDH2 protein. The screened compound was subjected to mutational analysis accomplishing second-site mutations. Interestingly, squalene exhibited appreciable binding affinity alongside good brain penetrating potential than enasidenib. Indeed, the reproducibility and significance of our results are examined by running 3 replica 100ns simulations per system using the random initial velocities of the atoms generated by Maxwell distribution at a given temperature. Collectively, the results from our study speculate that squalene could be persuasively translated for its use as a rational therapeutic agent against mIDH2 variants.
Comprehensive Analysis of Pyroptosis-related Genes for the Prognosis of Esophageal Cancer
Authors: Zhou Xiaoying , Li Wenjie , Xia Peichen , Chen Han , Su Xin , Li Xinyan , Jiang Xingzhou , Zhang Guoxin 
Published: July 2022

Abstract
Background
Esophageal cancer (EC), known for its high malignancy and difficulty in treatment, has seen a significant increase in incidence in recent years. Studies on prognosis of EC are extremely urgent to provide personalized medicine which will benefit more patients individually. Pyroptosis, an inflammatory form of programmed cell death triggered by caspase-1/4/5/11 which is activated by some inflammasomes has been connected to cancer in recent studies. However, the association between pyroptosis-related genes and esophageal cancer has not been elucidated, and their vital role in the development and prognosis of esophageal cancer needs further research.
Methods
We obtained 290 samples from both TCGA and GEO database,including 11 normal people and 279 EC patients. Then we screened pyroptosis-related regulators that were differentially expressed in normal esophageal and cancer tissues. We next exerted univariate Cox regression analysis and LASSO analysis to further choose key genes.Validation of these genes’ expression in cells was also conducted in vitro. Using these genes and other clinical features, we next constructed a risk model and a nomogram for predicting the prognosis of EC patients. Functions of pyroptosis-related genes and their relevance to clinical characteristics were discussed through two clustering analyses, Finally, we constructed a pyroptosis score system to predict the prognosis of EC.
Results
We finally screened 32 differentially expressed genes between normal and tumor tissues.And 8 key genes were identified after univariate Cox regression and LASSO analysis. Then the risk model and nomogram built based on them both showed strong predictive power according to our survival curves and ROC curves.Our subsequent invitro validation on key genes above also appeared high agreement with the regression analysis result. Finally, we calculated the pyroptosis score and it showed strong relationship with clinical features.
Conclusion
Pyroptosis-related genes are intimately related with EC and can be used to predict the prognosis of EC, which may also provide novel therapeutic schedule for EC patients.
Validated LC-MS/MS method for the simultaneous analysis of veterinary drugs in cultured meat media
Authors: Gu Song-Yi , Kim Jong Jib , Lee Jongsoo , Park Kyung-Ho , Lee Sang-Yoon , Kim Sang-Gu 
Published: July 2022

Abstract
The development of alternative meat, such as cultured meat, is of current interest due to its potential to solve various problems common to the livestock industry. Cultured meat is defined as in vitro, lab-grown, or synthetic meat, and is produced through the differentiation and culture of animal cells. To increase the efficiency of this culture veterinary drugs are commonly employed. It is therefore necessary to develop a method that allows the amounts of veterinary drugs, growth-promoting hormones, and antibiotics to be determined in cultured meat, to ultimately ensure an adequate safety profile. In this study, we report the development of an analytical method based on liquid chromatography–tandem mass spectrometry for the simultaneous analysis of various veterinary drugs. Validation of the developed method indicated a high accuracy and precision, in addition to a linearity of 0.9868–0.9999. Using this method, the veterinary drugs present in samples of culture media were analyzed. Importantly, penicillin was detected in the range of 2 to 44,259 mg/L, and no other veterinary drugs were detected. It is therefore expected the implementation of this method will improve the safety profile of the cultured meat market, in addition to securing the safety of new food technologies.
Multimodal bubble microrobot near free liquid surface
Authors: Wang Leilei , Chen Li , Zheng Xu , Yu Zexiong , Lv Wenchao , Sheng Minjia , Wang Lina , Nie Pengcheng , Li Hangyu , Guan Dongshi , CUI Haihang 
Published: July 2022

Abstract
The development of multifunctional and robust swimming microrobots working at the free air-liquid interface has encountered challenge as new manipulation strategy are needed to overcome the complicated interfacial restrictions. Here, we show flexible but reliable mechanisms to achieve a remote-control bubble microrobot with multiple working modes and high maneuverability by the assistance of a soft air-liquid interface. This bubble microrobot is developed from a hollow Janus microsphere regulated by a magnetic field, which can implement switchable working modes like pusher, gripper, anchor and sweeper. The cavitation of the microbubble and the accompanying directional jet flow play a key role for functioning in these working modes, which is analogues to "bubble tentacle". In particular, we find a novel speed modulation method for the bubble microrobot that does not need to change the fuel concentration. Our findings demonstrate a substantial advance of the bubble microrobot specifically working at the air-liquid interface. And the results depict the nonintuitive mechanisms, such as bubble dynamics confined by a free surface, hydrodynamic jetting and surface capillary wave induced by bubble collapse, and bubble particle interaction, which offer helpful insights for developing swimming microrobots working in complicated environments.
An integrated analysis of whole transcriptome and epigenome data reveals ENSR00000272060, an enhancer RNA, as a potential diagnostic biomarker in gastric cancer
Authors: Bahrami Basireh , Wolfien Markus , Nikpour Parvaneh 
Published: July 2022

Abstract
Enhancer RNAs (eRNAs) are a type of non-coding RNAs that are involved in gene expression regulation. Although altered expression and functional roles of eRNAs in the pathophysiology of neoplasms have been reported, their role in gastric cancer (GC), one of the most common and lethal cancers, is less known. In this work, we utilized a network-based approach for analyzing transcriptome and epigenome alteration in GC using The Cancer Genome Atlas (TCGA) data portal. A hub eRNA from the network: ENSR00000272060, showed a significantly increased expression in GC tumoral compared to the non-tumoral tissues, as well as an association with prognosis-related clinicopathological features. The constructed regulatory network of eRNAs and target genes in GC presents a comprehensive understanding of the underlying processes involved in tumor development, as well as a potential start to derive diagnostic or prognostic biomarkers.
Efficacy of subthreshold trabeculoplasty with micropulse laser in the treatment of primary open angle glaucoma
Authors: Parolini Barbara , Sahyoun Jean-Yves , Carbognin Sara , Tozzi Luigi , Gius irene , Greggio Angelo , Frisina Rino 
Published: July 2022

Abstract
Purpose
To report the results of subthreshold micropulse laser trabeculoplasty (MLT) in a consecutive series of patients, affected by primary open angle glaucoma (POAG).
Methods
In this single-center, one-arm, prospective study conducted on sixty-eight patients (112 eyes) affected by POAG, who underwent 360° 577-nm MLT. Follow-up consultations were scheduled at 1, 6, 12, 18 and 24 months. At baseline and at each consultation after MLT, data were collected on IOP, best corrected visual acuity, local and systemic side effects, number of eye-drops in use.
Results
One-hundred and twelve eyes of 68 patients were enrolled. Mean age was 57.68 ± 8.0 years, mean baseline IOP was 18.4 ± 5.2 mmHg with 1.43 ± 0.85 glaucoma medications. Treatment with MLT resulted in significantly lower IOP at both 1 month (18.4 ± 5.2mmHg, p = 0.045) and 24 months (18.4 ± 5.2 mmHg; p = 0.046) compared to baseline. No statistically significant difference was found between IOP at 1 month and at 12 months and between IOP at 1 month and at 24 months after treatment (p > 0.05). A low rate of retreatments was observed, mostly performed after 18 months from baseline. Most of the eyes were free of additional medical therapy at 12 months (88.9%) and 24 months (94.3%). No local or systemic side effects during or after MLT were reported.
Conclusion
This is the first prospective study to report long-term outcomes of MLT in POAG. MLT is safe and effective with an efficacy achieved within the first month and maintained after 2 years of treatment.