bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wear and tear of the intestinal visceral musculature by intrinsic and extrinsic factors
Authors: Kim Ho D. , So Eric , Lee Jiae , Wang Yi , Gill Vikram S. , Gorbacheva Anna , Han Hee Jin , Ng Katelyn G.‐L. , Ning Ken , Pranoto Inez K. A. , Cabrera Alejandra J. H. , Eom Dae Seok , Kwon Young V. 
Published: March 2022

AbstractBackgroundThe gut visceral musculature plays essential roles in not only moving substances through the lumen but also maintaining the function and physiology of the gut. Although the development of the visceral musculature has been studied in multiple model organisms, how it degenerates is poorly understood.ResultsHere, we employ the Drosophila midgut as a model to demonstrate that the visceral musculature is disrupted by intrinsic and extrinsic factors, such as aging, feeding, chemical‐induced tissue damage, and oncogenic transformation in the epithelium. Notably, we define four prominent visceral musculature disruption phenotypes, which we refer as “sprout,” “discontinuity,” “furcation,” and “crossover” of the longitudinal muscle. Given that the occurrence of these phenotypes is increased during aging and under various stresses, we propose that these phenotypes can be used as quantitative readouts of deterioration of the visceral musculature. Intriguingly, administration of a tissue‐damaging chemical dextran sulfate sodium (DSS) induced similar visceral musculature disruption phenotypes in zebrafish larvae, indicating that ingestion of a tissue‐damaging chemical can disrupt the visceral musculature in a vertebrate as well.ConclusionsOur study provides insights into the deterioration of the gut visceral musculature and lays a groundwork for investigating the underlying mechanisms in Drosophila as well as other animals.
Effectiveness of acoustic lures for increasing tropical forest understory bat captures
Authors: Aylen Oliver , Bishop Philip J. , bin Haji Abd Wahab Rodzay , Grafe T. Ulmar 
Published: March 2022

AbstractBats are the most diverse mammalian order second to rodents, with 1400+ species globally. In the tropics, it is possible to find more than 60 bat species at a single site. However, monitoring bats is challenging due to their small size, ability to fly, cryptic nature, and nocturnal activity. Recently, bioacoustic techniques have been incorporated into survey methods, either through passive acoustic monitoring or acoustic bat lures. Lures have been developed on the premise that broadcasting acoustic stimuli increases the number of captures in harp traps or mist nets. However, this is a relatively new, niche method. This study tested the efficacy of two commonly used acoustic bat lure devices, broadcasting two different acoustic stimuli, to increase forest understory bat captures in the tropics. This is the first time an acoustic bat lure has been systematically tested in a tropical rainforest, and the first study to compare two lure devices (Sussex AutoBat and Apodemus BatLure). Using a paired experimental design, two synthesized acoustic stimuli were broadcasted, a feeding call and a social call, to understand the importance of the call type used on capture rates and genus‐specific responses. Using an acoustic lure significantly increased capture rates, while the type of device did not impact capture rates. The two acoustic stimuli had an almost even distribution of captures, suggesting that the type of call may be less important than previously thought. Results indicate a possible deterrent effect on Rhinolophous sp., while being particularly effective for attracting bats in the genera Murina and Kerivoula. This study highlights the effectiveness of lures, however, also indicates that lure effects can vary across genera. Therefore, lures may bias survey results by altering the species composition of bats caught. Future research should focus on a single species or genus, using synthesized calls of conspecifics, to fully understand the effect of lures.
Flt3L, LIF, and IL‐10 combination promotes the selective in vitro development of ESAMlow cDC2B from murine bone marrow
Authors: Cyran Laura , Serfling Julia , Kirschner Luisa , Raifer Hartmann , Lohoff Michael , Hermanns Heike M. , Kerstan Andreas , Bodem Jochen , Lutz Manfred B. 
Published: March 2022

AbstractThe development of two conventional dendritic cells (DC) subsets (cDC1 and cDC2) and the plasmacytoid DC (pDC) in vivo and in cultures of bone marrow (BM) cells is mediated by the growth factor Flt3L. However, little is known about the factors that direct the development of the individual DC subsets. Here, we describe the selective in vitro generation of murine ESAMlow CD103− XCR1− CD172a+ CD11b+ cDC2 from BM by treatment with a combination of Flt3L, LIF, and IL‐10 (collectively named as FL10). FL10 promotes common dendritic cell progenitors (CDP) proliferation in the cultures, similar to Flt3L and CDP sorted and cultured in FL10 generate exclusively cDC2. These cDC2 express the transcription factors Irf4, Klf4, and Notch2, and their growth is reduced using BM from Irf4−/− mice, but the expression of Batf3 and Tcf4 is low. Functionally they respond to TLR3, TLR4, and TLR9 signals by upregulation of the surface maturation markers MHC II, CD80, CD86, and CD40, while they poorly secrete proinflammatory cytokines. Peptide presentation to TCR transgenic OT‐II cells induced proliferation and IFN‐γ production that was similar to GM‐CSF‐generated BM‐DC and higher than Flt3L‐generated DC. Together, our data support that FL10 culture of BM cells selectively promotes CDP‐derived ESAMlow cDC2 (cDC2B) development and survival in vitro.
Cookies Formulated with Gamma‐Irradiated Virgin Coconut Oil are Less Rancid: Analysis By Metal Oxide‐Based Electronic Nose and Support Vector Machines
Authors: Mondal Kona , Bose Anupama , Tamili Dipshikha , Chakraborty Soumi , Chatterjee Dipan , Paul Kaninika , Bhattacharyya Nabarun , Bhattacharjee Paramita 
Published: March 2022

AbstractIn this study, hydrogenated vegetable fat in cookie is replaced by gamma‐irradiation‐induced (4.2 kGy) deodorized (rancid‐acid odor removed) virgin coconut oil (VCO) with the aim to formulate antioxidant‐rich cookies with good sensory acceptability up to 150 days,vis‐à‐visthe hydrogenated fat‐ and VCO‐formulated cookies. Cookies formulated with hydrogenated fat, non‐irradiated VCO and 4.2 kGy irradiated VCO serve as control, positive control, and sample sets, respectively. Rancidity of three sets of cookies is assessed for a period of 180 days at 25±2°C, ≈75–80% RH using metal oxide gas sensor‐based electronic nose (e‐nose). The best combinations of sensors are selected employing support vector machines analysis to accurately predict rancidity development in the three sets of cookies during storage. Analyses of principal component analysis (PCA) plots of the e‐nose sensor responses reveal that the progression of rancidity in the control and positive control sets of cookies is detected on day 90 and 120, respectively; whereas, the onset of rancidity is detected for sample set of cookies on day 150. Findings of sensory evaluation and e‐nose analyses affirm that the newly formulated cookies can be safely consumed up to 150 days when stored at 25±2 °C, 75–80% RH. Practical applications: Gamma‐irradiated deodorized VCO successfully enhances storage‐life of drop cookies by more than 60 daysvis‐à‐visits hydrogenated fat counterpart. These newly formulated cookies which can be stored and consumed for a longer period would certainly be more commercially viable. Gamma‐irradiated VCO can also be used as an alternative to hydrogenated fats in preparing similar bakery‐cum‐confectionary products. The methodology developed for assessment of rancidity using electronic nose technology would be extremely useful considering high industrial throughput and can be safely extrapolated to other bakery and confectionary products for accurate assessment of rancidity, forgoing cumbersome biochemical assays.
A compact microfluidic geometry for multiplexing enzyme‐linked immunosorbent assays
Authors: Giri Basant , Dutta Debashis 
Published: March 2022

AbstractWe have previously reported a novel approach to implementing multiplex enzyme‐linked immunosorbent assay (ELISA) in connected microchannels by exploiting the slow diffusion of the enzyme reaction product across the different assay segments. This work builds on that report by implementing the noted assay in segments arranged along the circumference of a circular channel layout to reduce the footprint size and sample volume requirement. Using the current design, a 5‐plex cytokine ELISA was demonstrated in a 1.5 × 1.5‐cm region, which corresponded to a reduction in the footprint area by about a factor of 3 compared to that reported in our previous study. Additionally, the selective coating of our assay segments with the target molecules was realized in this work using electroosmosis instead of hydrodynamic flow as was the case in the previous report. This aspect of our experimental design is particularly significant as it permits the use of cross‐sectional channel dimensions significantly shorter than those employed in the current work. Moreover, the use of an electric field for coating purposes enables the integration of functionalities such as electrokinetic preconcentration of analyte molecules during the sample incubation period that can further enhance the capabilities of our assay method.
Scalp HFO rates are higher for larger lesions
Authors: Cserpan Dorottya , Gennari Antonio , Gaito Luca , Lo Biundo Santo Pietro , Tuura Ruth , Sarnthein Johannes , Ramantani Georgia 
Published: March 2022

AbstractHigh‐frequency oscillations (HFO) in scalp EEG are a new and promising noninvasive epilepsy biomarker, providing added prognostic value, particularly in pediatric lesional epilepsy. However, it is unclear if lesion characteristics, such as lesion volume, depth, type, and localization, impact scalp HFO rates. We analyzed scalp EEG from 13 children and adolescents with focal epilepsy associated with focal cortical dysplasia (FCD), low‐grade tumors, or hippocampal sclerosis. We applied a validated automated detector to determine HFO rates in bipolar channels. We identified the lesion characteristics in MRI. Larger lesions defined by MRI volumetric analysis corresponded to higher cumulative scalp HFO rates (P = .01) that were detectable in a higher number of channels (P = .05). Both superficial and deep lesions generated HFO detectable in the scalp EEG. Lesion type (FCD vs tumor) and lobar localization (temporal vs extratemporal) did not affect scalp HFO rates in our study. Our observations support that all lesions may generate HFO detectable in scalp EEG, irrespective of their characteristics, whereas larger epileptogenic lesions generate higher scalp HFO rates over larger areas that are thus more accessible to detection. Our study provides crucial insight into scalp HFO detectability in pediatric lesional epilepsy, facilitating their implementation as an epilepsy biomarker in a clinical setting.
Recognition and promotion of faculty work: Practices emerging at the intersection between faculty development and educational renewal
Authors: Lutz Christel 
Published: March 2022

AbstractHow can we change the conversation about faculty work? Informed by recent changes in higher education in the Netherlands, this chapter highlights three areas that are at the intersection between faculty development and educational renewal as salient for supporting mid‐career faculty—addressing first the emerging opportunities and responsibilities for individual faculty members, then for teams, and finally for institutional leaders.
Analysis of direct‐acting antiviral‐resistant hepatitis C virus haplotype diversity by single‐molecule and long‐read sequencing
Authors: Yamauchi Kozue , Sato Mitsuaki , Osawa Leona , Matsuda Shuya , Komiyama Yasuyuki , Nakakuki Natsuko , Takada Hitomi , Katoh Ryo , Muraoka Masaru , Suzuki Yuichiro , Tatsumi Akihisa , Miura Mika , Takano Shinichi , Amemiya Fumitake , Fukasawa Mitsuharu , Nakayama Yasuhiro , Yamaguchi Tatsuya , Inoue Taisuke , Maekawa Shinya , Enomoto Nobuyuki 
Published: March 2022

Abstract
The method of analyzing individual resistant hepatitis C virus (HCV) by a combination of haplotyping and resistance‐associated substitution (RAS) has not been fully elucidated because conventional sequencing has only yielded short and fragmented viral genomes. We performed haplotype analysis of HCV mutations in 12 asunaprevir/daclatasvir treatment‐failure cases using the Oxford Nanopore sequencer. This enabled single‐molecule long‐read sequencing using rolling circle amplification (RCA) for correction of the sequencing error. RCA of the circularized reverse‐transcription polymerase chain reaction products successfully produced DNA longer than 30 kilobase pairs (kb) containing multiple tandem repeats of a target 3 kb HCV genome. The long‐read sequencing of these RCA products could determine the original sequence of the target single molecule as the consensus nucleotide sequence of the tandem repeats and revealed the presence of multiple viral haplotypes with the combination of various mutations in each host. In addition to already known signature RASs, such as NS3‐D168 and NS5A‐L31/Y93, there were various RASs specific to a different haplotype after treatment failure. The distribution of viral haplotype changed over time; some haplotypes disappeared without acquiring resistant mutations, and other haplotypes, which were not observed before treatment, appeared after treatment. Conclusion: The combination of various mutations other than the known signature RAS was suggested to influence the kinetics of individual HCV quasispecies in the direct‐acting antiviral treatment. HCV haplotype dynamic analysis will provide novel information on the role of HCV diversity within the host, which will be useful for elucidating the pathological mechanism of HCV‐related diseases.
Work transformation and the HR ecosystem dynamics: A longitudinal case study of HRM disruption in the era of the 4th industrial revolution
Authors: Yalenios Jocelyne , d'Armagnac Sophie 
Published: March 2022

AbstractDisruption in human resource management (HRM) practices necessitates processes of mutual adjustment within the organization that seeks to address these changes properly, overcome tensions, and fit strategic needs. In our single longitudinal case study of a new HRM practice development concerning blue‐collar worker talent management, we examine how HR professionals and managers interact and develop new HRM practices as a response to disruptive work transformation. Considering these interactions from the perspective of HR ecosystem alignment dynamics, we find that both managers and HR professionals engaged in fruitful collaboration processes along three differentiated steps to provide value for the whole organization. We propose a grounded theorizing of HR ecosystem alignment that is based on the progression of successive convergent and divergent phases and introducing collaborative spaces of work.
Non‐Newtonian nanofluid characteristics over a melting wedge: A numerical study
Authors: Dharmaiah G. , Sridhar W. , AL‐Farhany Khaled , Balamurugan K. S. , Ali Farhan 
Published: March 2022

AbstractA study is conducted to determine the impact of joule heating, thermo‐diffusion, and chemical reaction effect on wedge flow with melting. Using similarity transformation, the nonlinear PDEs regulate nanofluid flow is converted to nonlinear ODEs. The MATLAB solver is used to solve the boundary value problem numerically. The interaction of relevant physical entities on nanoparticle concentration, nanofluid temperature, nanofluid velocity, skin friction, rate of heat, and mass transfer is graphically portrayed. This study will aid in the development of cooling devices and various shapes in heat sinks, as well as improving the heat transfer characteristics of Casson flow and strengthening formerly industrial uses. In the limiting situation, current findings are compared to analysis of findings. Flow velocity and concentration compacts in association with enhancing values of chemical reaction factor while temperature increases with enhancing the values of chemical reaction parameter. An upsurge in the temperature of the fluid is seen with the increasing Eckert number. It is found that the melting process increases the thicknesses of Solutal, thermal, and momentum boundary layers while it reduces mass transfer rate, heat transfer rate, and Skin friction. The Casson fluid displays a superior heat transfer mechanism than the Newtonian fluid. This study would be valuable in designing cooling gadgets and heat sinks of various shapes which will enhance the heat transfer properties of Casson nanofluids thereby increasing their applications in industrial perspectives. Moreover, the study reveals the novel applications of Casson nanofluids in cooling devices and heat sinks.
Numerical scrutinization of dropwise condensation heat transfer on an inclined surface
Authors: Mohammadpour Loghman , Moghadasi Hesam , Saffari Hamid 
Published: March 2022

AbstractRecently, achieving an ameliorated heat transfer rate in dropwise condensation (DWC) has attracted the attention of many researchers. Several parameters, including chemical and physical properties of the substrate, inclination, and interfacial characteristics influence DWC heat transfer rate. The variation of inclination angle is followed by the change in droplet shape and consequently, the heat transfer rate are changed. In this study, the effect of droplet shape variation on diverse inclined substrates is simulated. Moreover, three‐dimensional mass, momentum, and energy equations considering the desired boundary conditions on the unstructured grid are utilized for the scrutinization of flow behavior and heat transfer for static and sliding droplets. For the sake of validation, the outcomes obtained from the simulation were compared with existent data in the literature and a proper agreement was attained. Regarding the outcomes, it was of concern that the influence of inclination angle on the droplet shape is more distinct at higher droplet volume; while no considerable change was seen on heat flux of small droplets by increasing the inclination angle. Furthermore, a higher heat transfer rate was noted by exceeding the inclination angle beyond a definite angle. Additionally, the increased heat transfer rate was affirmed by increasing the Marangoni number .
Effects of ash derived from livestock manure and two other treatments on soil moisture content and water infiltration rate
Authors: Mortazavizadeh Fatemehsadat , Fatahi Amirali , Asefpour Vakilian Keyvan , Pagliari Paulo H. , Cerdà Artemi , Mirzaei Majid , Zhang Xiuju , Adnan Ikram Rana Muhammad 
Published: March 2022

AbstractThis study investigates the effects of using livestock manure (LM), livestock manure ash (LMA) and the combination of LM with LMA (LM + LMA) on the soil moisture content and water infiltration rate of clay, clay loam and sandy loam soils. The soil moisture content and water infiltration rate were 11.60, 8.90 and 6.85% and 1.98, 1.55 and 1.62 cm h‾¹ for clay, clay loam and sandy loam soils, respectively, by the application of 15 t ha‾¹ LM. Moreover, using 15 t ha‾¹ of LMA had the most desirable effects on the soil moisture content in the clay and clay loam soils. Using this treatment, the soil moisture content and water infiltration rate were 10.85, 11.20 and 7.24% and 2.40, 1.90 and 1.75 cm h‾¹ for clay, clay loam and sandy loam soils, respectively. Promising results were obtained using 15 + 15 t ha‾¹ of LM + LMA, where the soil moisture content and water infiltration rate were 11.50, 10.20 and 8.20% and 2.80, 1.90 and 1.72 cm h‾¹ for clay, clay loam and sandy loam soils, respectively. Both LMA and its combination with LM were found to be positive strategies to achieve sustainable agricultural goals because of the increased water infiltrates (less runoff) available for the plants.
Medium‐ to long‐term forecast of reference crop evapotranspiration based on the correction of the principal control factor
Authors: Gong Liqin , Peng Zhigong , Wei Zheng , Zhang Baozhong , Dong Jie , Chen He , Cai Jiabing , Zhang Qian , Zhang Feng 
Published: March 2022

AbstractThis study selected the temperature Penman–Monteith (PMT) model to solve the difficulty of reference crop evapotranspiration (ET0) prediction caused by incomplete meteorological data. Then, the principal control factor of the PMT model was determined using several indicators. The results show that the annual average correlation and sensitivity coefficients between Tmax and ET0 are the highest. Hence, among the factors, Tmax is the largest contributor in contribution proportion and amount to ET0 and the principal control factor of the PMT model. The root mean square error (RMSE), relative error (RE) and mean absolute error (MAE) of the forecast ET0 increased by 0.7, 0.2, and 0.6 mm/day, respectively, after the 16th day compared with those on the 15th day. Thus, a remarkable drop in ET0 forecast precision is observed and is hardly accurate for the purpose of water distribution management in an irrigation district. The RMSE, RE, and MAE of the Tmax forecast decrease by 2.0–3.0°C, 0.1–0.2, and 2.0–2.8°C, respectively, and those of the corresponding ET0 forecast decrease by 0.7–0.9, 0.2–0.3, and 0.6–0.7 mm/day, respectively, after Tmax correction. Compared with the decaying‐average method, the improved decaying‐average method can correct Tmax better and improve the ET0 prediction accuracy.
Population Pharmacokinetics of Z‐Endoxifen in Patients With Advanced Solid Tumors
Authors: Koubek Emily J. , Ralya Andrew T. , Larson Thomas R. , McGovern Renee M. , Buhrow Sarah A. , Covey Joseph M. , Adjei Alex A. , Takebe Naoko , Ames Matthew M. , Goetz Matthew P. , Reid Joel M. 
Published: March 2022

AbstractThe purpose of this study was to develop and validate a population pharmacokinetic model for Z‐endoxifen in patients with advanced solid tumors and to identify clinical variables that influence pharmacokinetic parameters. Z‐endoxifen‐HCl was administered orally once a day on a 28‐day cycle (±3 days) over 11 dose levels ranging from 20 to 360 mg. A total of 1256 Z‐endoxifen plasma concentration samples from 80 patients were analyzed using nonlinear mixed‐effects modeling to develop a population pharmacokinetic model for Z‐endoxifen. A 2‐compartment model with oral depot and linear elimination adequately described the data. The estimated apparent total clearance, apparent central volume of distribution, and apparent peripheral volume of distribution were 4.89 L/h, 323 L, and 39.7 L, respectively, with weight‐effect exponents of 0.75, 1, and 1, respectively. This model was used to explore the effects of clinical and demographic variables on Z‐endoxifen pharmacokinetics. Weight, race on clearance, and aspartate aminotransferase on the absorption rate constant were identified as significant covariates in the final model. This novel population pharmacokinetic model provides insight regarding factors that may affect the pharmacokinetics of Z‐endoxifen and may assist in the design of future clinical trials.