bluuebunny/crossref_metadata_2025_split huggingface dataset:
An inosine triphosphate pyrophosphatase safeguards plant nucleic acids from aberrant purine nucleotides
Authors: Straube Henryk , Straube Jannis , Rinne Jannis , Fischer Lisa , Niehaus Markus , Witte Claus‐Peter , Herde Marco 
Published: December 2022

Summary
In plants, inosine is enzymatically introduced in some tRNAs, but not in other RNAs or DNA. Nonetheless, our data show that RNA and DNA from Arabidopsis thaliana contain (deoxy)inosine, probably derived from nonenzymatic adenosine deamination in nucleic acids and usage of (deoxy)inosine triphosphate (dITP and ITP) during nucleic acid synthesis.
We combined biochemical approaches, LC–MS, as well as RNA‐Seq to characterize a plant INOSINE TRIPHOSPHATE PYROPHOSPHATASE (ITPA) from A. thaliana, which is conserved in many organisms, and investigated the sources of deaminated purine nucleotides in plants.
Inosine triphosphate pyrophosphatase dephosphorylates deaminated nucleoside di‐ and triphosphates to the respective monophosphates. ITPA loss‐of‐function causes inosine di‐ and triphosphate accumulation in vivo and an elevated inosine and deoxyinosine content in RNA and DNA, respectively, as well as salicylic acid (SA) accumulation, early senescence, and upregulation of transcripts associated with immunity and senescence. Cadmium‐induced oxidative stress and biochemical inhibition of the INOSINE MONOPHOSPHATE DEHYDROGENASE leads to more IDP and ITP in the wild‐type (WT), and this effect is enhanced in itpa mutants, suggesting that ITP originates from ATP deamination and IMP phosphorylation.
Inosine triphosphate pyrophosphatase is part of a molecular protection system in plants, preventing the accumulation of (d)ITP and its usage for nucleic acid synthesis.
Mitochondrial alternative NADH dehydrogenases NDA1 and NDA2 promote survival of reoxygenation stress in Arabidopsis by safeguarding photosynthesis and limiting ROS generation
Authors: Jethva Jay , Lichtenauer Sophie , Schmidt‐Schippers Romy , Steffen‐Heins Anja , Poschet Gernot , Wirtz Markus , van Dongen Joost T. , Eirich Jürgen , Finkemeier Iris , Bilger Wolfgang , Schwarzländer Markus , Sauter Margret 
Published: December 2022

Summary

Plant submergence stress is a growing problem for global agriculture. During desubmergence, rising O2 concentrations meet a highly reduced mitochondrial electron transport chain (mETC) in the cells. This combination favors the generation of reactive oxygen species (ROS) by the mitochondria, which at excess can cause damage. The cellular mechanisms underpinning the management of reoxygenation stress are not fully understood.

We investigated the role of alternative NADH dehydrogenases (NDs), as components of the alternative mETC in Arabidopsis, in anoxia–reoxygenation stress management.

Simultaneous loss of the matrix‐facing NDs, NDA1 and NDA2, decreased seedling survival after reoxygenation, while overexpression increased survival. The absence of NDAs led to reduced maximum potential quantum efficiency of photosystem II linking the alternative mETC to photosynthetic function in the chloroplast. NDA1 and NDA2 were induced upon reoxygenation, and transcriptional activation of NDA1 was controlled by the transcription factors ANAC016 and ANAC017 that bind to the mitochondrial dysfunction motif (MDM) in the NDA1 promoter. The absence of NDA1 and NDA2 did not alter recovery of cytosolic ATP levels and NADH : NAD+ ratio at reoxygenation. Rather, the absence of NDAs led to elevated ROS production, while their overexpression limited ROS.

Our observations indicate that the control of ROS formation by the alternative mETC is important for photosynthetic recovery and for seedling survival of anoxia–reoxygenation stress.
A cyclic lipopeptide produced by an antagonistic bacterium relies on its tail and transient receptor potential‐type Ca2+ channels to immobilize a green alga
Authors: Hou Yu , Bando Yuko , Carrasco Flores David , Hotter Vivien , Das Ritam , Schiweck Bastian , Melzer Tommy , Arndt Hans‐Dieter , Mittag Maria 
Published: December 2022

Summary
The antagonistic bacterium Pseudomonas protegens secretes the cyclic lipopeptide (CLiP) orfamide A, which triggers a Ca2+ signal causing rapid deflagellation of the microalga Chlamydomonas reinhardtii.
We performed chemical synthesis of orfamide A derivatives and used an aequorin reporter line to measure their Ca2+ responses. Immobilization of algae was studied using a modulator and mutants of transient receptor potential (TRP)‐type channels.
By investigating targeted synthetic orfamide A derivatives, we found that N‐terminal amino acids of the linear part and the terminal fatty acid region are important for the specificity of the Ca2+‐signal causing deflagellation. Molecular editing indicates that at least two distinct Ca2+‐signaling pathways are triggered. One is involved in deflagellation (Thr3 change, fatty acid tail shortened by 4C), whereas the other still causes an increase in cytosolic Ca2+ in the algal cells, but does not cause substantial deflagellation (Leu1 change, fatty acid hydroxylation, fatty acid changes by 2C). Using mutants, we define four TRP‐type channels that are involved in orfamide A signaling; only one (ADF1) responds additionally to low pH.
These results suggest that the linear part of the CLiP plays one major role in Ca2+ signaling, and that orfamide A uses a network of algal TRP‐type channels for deflagellation.
Two‐Year Clinical Outcomes after Arthroscopic Re‐Excision of Recurrent Dorsal Wrist Ganglion Cyst
Authors: Zheng Tong , Huang Xingjian , Yang Yong , Li Bin , Li Feng , Li Zhongzhe 
Published: December 2022

ObjectivesArthroscopic excision of dorsal wrist ganglion (DWG) cysts has recently become an alternative to open surgery, with the advantage of lower recurrence. However, in recurrent cases, whether re‐excision using an arthroscopic approach would achieve favorable outcomes has not been determined. This study aimed to evaluate the clinical outcomes of function evaluation and recurrence rate after arthroscopic excision of recurrent DWG cysts.MethodsA total of 11 consecutive patients with clinically diagnosed recurrent DWG cysts were retrospectively reviewed between November 2017 and March 2020. Extensive re‐excision of the ganglion cyst and its surrounding pathological capsule was performed using an arthroscopic approach. Magnetic resonance imaging (MRI) was routinely performed before surgery to identify the location and limits of the cyst. All patients were followed up for a minimum of 2 years. A second recurrence was recorded if a mass reappeared at the same site with a positive transillumination test. Pain during activity was evaluated using the visual analog scale (VAS). The active range of motion (ROM) of the wrist was measured using a goniometer, and the hand grip strength was measured using a digital dynamometer. Comparative analysis between the pre‐ and postoperative indexes was performed using Student's t‐test.ResultsAfter a mean follow‐up period of 29.3 months (range, 24–34 months), no second recurrence of the cyst was recorded. The VAS score improved from 1.4 to 0.3 (t = 3.833, p = 0.003), and residual pain was reported by three patients (VAS score = 1 for each). Active wrist flexion increased from 73.6° to 78.2° (t = 2.887, p = 0.016). No significant changes were found in active wrist extension or hand grip strength (p > 0.05). No major complications occurred during the study.ConclusionArthroscopic excision of a recurrent DWG cyst yielded satisfactory results with no second recurrence, significant pain relief, and good wrist function at a minimum of 2‐year follow‐up. Clear identification of the location and limits of the ganglion based on preoperative MRI could be helpful to achieve complete excision and therefore prevent a second recurrence.
Multicentre observational study on multisystem inflammatory syndrome related to COVID‐19 in Argentina
Authors: Vainstein Eduardo , Baleani Silvia , Urrutia Luis , Affranchino Nicolás , Ackerman Judith , Cazalas Mariana , Goldsman Alejandro , Sardella Angela , Tolin Ana Laura , Goldaracena Pablo , Fabi Mariana , Cosentino Mariana , Magliola Ricardo , Roggiero Gustavo , Manso Paula , Triguy Jésica , Ballester Celeste , Cervetto Vanesa , Vaccarello María , De Carli Domingo Norberto , De Carli Maria Estela , Ciotti Ana Laura , Sicurello María Irene , Rios Leiva Cecilia , Villalba Claudia , Hortas María , Peña Sonia , González Gabriela , Zold Camila Lidia , Murer Mario Gustavo ,   EMSKC Group
Published: December 2022

AbstractBackgroundThe impact of the pediatric inflammatory multisystem syndrome temporally associated with SARS‐CoV‐2 (PIMS‐TS) in low‐ and middle‐income countries remains poorly understood. Our aim was to understand the characteristics and outcomes of PIMS‐TS in Argentina.MethodsThis observational, prospective, and retrospective multicenter study enrolled patients younger than 18 years‐old manifesting PIMS‐TS, Kawasaki disease (KD) or Kawasaki shock syndrome (KSS) between March 2020 and May 2021. Patients were followed‐up until hospital discharge or death (one case). The primary outcome was pediatric intensive care unit (PICU) admission. Multiple logistic regression was used to identify variables predicting PICU admission.ResultsEighty‐one percent, 82%, and 14% of the 176 enrolled patients fulfilled the suspect case criteria for PIMS‐TS, KD, and KSS, respectively. Temporal association with SARS‐CoV‐2 was confirmed in 85% of the patients and 38% were admitted to the PICU. The more common clinical manifestations were fever, abdominal pain, rash, and conjunctival injection. Lymphopenia was more common among PICU‐admitted patients (87% vs. 51%, p < 0.0001), who also showed a lower platelet count and higher plasmatic levels of inflammatory and cardiac markers. Mitral valve insufficiency, left ventricular wall motion alterations, pericardial effusion, and coronary artery alterations were observed in 30%, 30%, 19.8%, and 18.6% of the patients, respectively. Days to initiation of treatment, rash, lymphopenia, and low platelet count were significant independent contributions to PICU admission.ConclusionRates of severe outcomes of PIMS‐TS in the present study agreed with those observed in high‐income countries. Together with other published studies, this work helps clinicians to better understand this novel clinical entity.
Allocation to pediatric recipients around the world: An IPTA global survey of current pediatric solid organ transplantation deceased donation allocation practices
Authors: Hernández Benabe Stefany , Batsis Irini , Dipchand Anne I. , Marks Stephen D. , McCulloch Mignon I. , Hsu Evelyn K. 
Published: December 2022

AbstractBackgroundThere has not been a comprehensive global survey of pediatric‐deceased donor allocation practices across all organs since the advent of deceased donor transplantation at the end of the 20th century. As an international community that is responsible for transplanting children, we set out to survey the existing landscape of allocation. We aimed to summarize current practices and provide a snapshot overview of deceased donor allocation practices to children across the world.MethodsThe International Registry in Organ Donation and Transplantation (IRODAT, www.irodat.org) was utilized to generate a list of all countries in the world, divided by continent, that performed transplantation. We reviewed the published literature, published allocation policy, individual website references and associated links to publicly available listed allocation policies. Following this, we utilized tools of communication, relationships, and international fellowship to confirm deceased donation pediatric centers and survey pediatric allocation practices for liver, kidney, heart, and lung across the world. We summarize pediatric allocation practices by organ when available using source documents, and personal communication when no source documents were available.ResultsThe majority of countries had either formal or informal policies directed toward minimizing organ distribution disparity among pediatric patients.ConclusionChildren have long‐term life to gain from organ donation yet continue to die while awaiting transplantation. We summarize global strategies that have been employed to provide meaningful and sustained benefit to children on the waitlist.
International Pediatric Transplant Association (IPTA) position statement supporting prioritizing pediatric recipients for deceased donor organ allocation
Authors: Freeman Michael A. , Botha Jean , Brewer Eileen , Damian Mihaela , Ettenger Robert , Gambetta Katheryn , Lefkowitz Debra S. , Ross Lainie Friedman , Superina Riccardo , McCulloch Mignon I. , Blydt‐Hansen Tom ,   for the IPTA Ethics Committee
Published: December 2022

AbstractA position statement of the International Pediatric Transplant Association endorsing prioritizing pediatric recipients for deceased donor organ allocation, examining the key ethical arguments that serve as the foundation for that position, and making specific policy recommendations to support prioritizing pediatric recipients for deceased donor organ allocation globally.
Melioristic genealogies and Indigenous philosophies
Authors: De Cruz Helen , De Smedt Johan 
Published: December 2022

AbstractAccording to Mary Midgley, philosophy is like plumbing: like the invisible entrails of an elaborate plumbing system, philosophical ideas respond to basic needs that are fundamental to human life. Melioristic projects in philosophy attempt to fix or reroute this plumbing. An obstacle to melioristic projects is that the sheer familiarity of the underlying philosophical ideas renders the plumbing invisible. Philosophical genealogies aim to overcome this by looking at the origins of our current concepts. We discuss philosophical concepts developed in Indigenous cultures as a source of inspiration for melioristic genealogy. Examining the philosophical concepts of these communities is useful because it gives us a better idea of the range of ethical, political, and metaphysical approaches that exist in the world. Members of western societies do not get a clear view of this range, in part because living in large groups presents its own constraints and challenges, which limit philosophical options. We argue that features of Indigenous philosophies, such as egalitarianism and care for one's natural environment, are not inevitable byproducts of Native material conditions and lifestyles, but that they are deliberate forms of conceptual engineering. We propose that comparative philosophy is an integral part of the genealogical project.
Core values for ideal civil servants: Service‐oriented, responsive and dedicated
Authors: Neo Sheeling , Grimmelikhuijsen Stephan , Tummers Lars 
Published: December 2022

AbstractWhat do citizens want? How do citizens think public servants should behave? Although such questions seem straightforward, little is known about the values citizens expect public servants to uphold. This paper therefore identifies such values through extensive coding of qualitative data from representative samples of United States (n = 395), Dutch (n = 369), and South Korean (n = 379) citizens. Surprisingly, and contrasting to assumptions in the literature on citizen satisfaction, citizens hardly value effectiveness, efficiency, and accountability. In contrast, we found that the top three universal values that citizens desire from public servants are serviceability, responsiveness, and dedication. These values are generic across countries, age, gender, and education level. However, some values are more important in some countries than in others. These differences show the influence of a country's longstanding public administrative tradition and its current situation. Our findings challenge scholars and government officials to rethink what citizens want from their civil servants.
The system is broken: Can we have some more?
Authors: Lee Kyung Suk , Goidel Kirby , Young Clifford 
Published: December 2022

AbstractObjectiveIn this article, we explore how populist beliefs and related attitudes (nativism and system‐level discontent) are associated with support for government spending across a wide range of countries and spending categories.MethodUsing cross‐sectional survey data spanning 18 democratic countries, we test how each of these constructs affects support for government spending on education, healthcare, social welfare, and job creation, and how that support is affected by explicit tradeoffs between spending increases, taxation, and public debt.ResultsPopulism and system‐level discontent are associated with support for increased government spending, while nativism is associated with opposition to government spending.ConclusionsThis creates an inherent and perhaps intractable tension within right‐wing populist movements. The nativist sentiments that animate right‐wing populist movements work to undermine support for redistributive spending.
The deep radiomic analytics pipeline
Authors: Currie Geoff , Rohren Eric 
Published: December 2022

AbstractRadiomics refers to the process of extracting useful imaging features from radiological data. Conventional radiomics like standard uptake value, intensity histograms, or phase images involve hand‐crafted (manual) or automated regions of interest (computer generated), however, artificial intelligence (AI) segmentation (AI‐augmented radiomics) has recently emerged. Radiomic feature extraction extends image insights beyond simply data quantitation and provides additional insights to aid semantic reporting. Deeper layers of a convolutional neural network produce more abstract radiomic features that are referred to as deep radiomics. The application of radiomics in veterinary radiology is already firmly entrenched using hand‐crafted and automated computer‐generated radiomic features in X‐ray, nuclear medicine, CT, ultrasound, and MRI. There is an opportunity for veterinary radiology to capitalize on advances in AI, machine learning, and deep learning to enrich imaging interpretation using deep radiomic feature extraction. This manuscript aims to provide a general understanding of radiomics and deep radiomics, and to arm readers with the vernacular to progress discussion and development of deep radiomics in veterinary imaging.
Ecological determinants and risk areas of Striga hermonthica infestation in western Kenya under changing climate
Authors: Kimathi Emily , Abdel‐Rahman Elfatih M. , Lukhoba Catherine , Ndambi Asaah , Mudereri Bester Tawona , Niassy Saliou , Tonnang Henri E. Z. , Landmann Tobias 
Published: December 2022

AbstractStriga hermonthica (Del.) Benth is a parasitic weed that is damaging major cereal crops in sub‐Saharan Africa (SSA). Although Striga is recognised as an agricultural scourge, there is limited information available indicating the extent of its growth and spread as impacted by the changing climate in Kenya. This study investigated the impact of current climate conditions and projected future (2050) climate change on the infestation of Striga hermonthica in the western Kenya region. Specifically, the study aimed to predict Striga hermonthica habitat suitability in five counties in the western Kenya region through using the maximum entropy (MaxEnt) model and bioclimatic, soil, topographic and land use, and land cover (LULC) variables. Striga hermonthica geolocations were collected and collated and ecological niche models were developed to determine the habitat suitability. The results showed that approximately 1767 km2 (10% of the total study area) is currently highly suitable for Striga hermonthica occurrence. The future projections showed a range between 2106 km2 (19% of the total study area) and 2712 km2 (53% of the total study area) at the minimum carbon (RCP 2.6) and the maximum carbon emission scenarios (RCP 8.5) respectively. Elevation, annual precipitation, LULC, temperature seasonality and soil type were determined to be the most influential ecological predictor variables for Striga hermonthica establishment. The study revealed the importance of using climate, soil, topographic and LULC variables when evaluating agricultural production constraints such as Striga's prevalence. The methodology used in this study should be tested in other Striga affected areas.
Assessment of Dynamic Photovoltaic Shading Systems on Energy Performance of Commercial Buildings
Authors: Alwelayti Dana , Dehwah Ammar H. A. , Krarti Moncef 
Published: December 2022

Abstract
In this paper, the energy benefits of dynamic photovoltaic (PV)-integrated overhang systems are evaluated for office buildings. The benefits of dynamic PV-integrated overhangs combined energy efficiency by decreasing both heating and cooling energy demand and on-site renewable power generation by deploying tracking PV panels. The energy performance of dynamic PV-integrated overhangs is considered for various design and operation conditions. Specifically, the impacts of various design features are considered such as overhang depth, window size, glazing type, and heating, ventilation, and air conditioning (HVAC) system type. Moreover, the effects of operating conditions are assessed including the location of the building and the control strategy of the dynamic PV-integrated systems such as load-tracking, demand-tracking, and PV-tracking. The results of the various analyses indicate that the heating and cooling energy demands of the dynamic overhangs are mostly affected by the window glazing type and window overhang depths. The analysis shows that the use of demand-tracking maximizes the energy performance of the dynamic PV-integrated overhangs when deployed to US office buildings.
Highly Accurate Wavelet Solution for Bending and Free Vibration of Circular Plates Over Extra-Wide Ranges of Deflections
Authors: Liu Xiaojing , Zhou Youhe , Wang Jizeng 
Published: December 2022

Abstract
The wavelet multiresolution interpolation Galerkin method in which both the unknown functions and nonlinear terms are approximated by their respective projections onto the same wavelet space is utilized to implement the spatial discretization of the highly coupled and nonlinear Von Karman equation for thin circular plates with various types of boundary conditions and external loads. Newton’s method and the assumption of a single harmonic response are then used for solving the static bending and free vibration problems, respectively. Highly accurate wavelet solutions for an extremely wide range of deflections are finally obtained by the proposed method. These results for moderately large deflections are in good agreement with existing solutions. Meanwhile, the other results for larger deflections are rarely achieved by using other methods. Comparative studies also demonstrate that the present wavelet method has higher accuracy and lower computational cost than many existing methods for solving geometrically nonlinear problems of thin circular plates. Moreover, the solutions for large deflection problems with concentrated load support the satisfactory capacity for handling singularity of the proposed wavelet method. In addition, a trivial initial guess, such as zero, can always lead to a convergent solution in very few iterations, even when the deflection is as large as over 46 times thickness of plate, showing an excellent convergence and stability of the present wavelet method in solving highly nonlinear problems.