bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ovarian status modulates endocrine and neuroinflammatory responses to a murine mammary tumor
Authors: Strehle Lindsay D. , Russart Kathryn L.G. , Burch Valerie , Grant Corena V. , Pyter Leah M. 
Published: April 2022

Abstract
In breast cancer patients, behavioral comorbidities reduce quality of life, increase mortality, and may persist years into remission. These side effects are associated with increased circulating and brain inflammatory markers in patients and rodent tumor models, respectively. The prevalence and severity of these behavioral comorbidities stratify by menopausal status, suggesting an underlying mechanistic role of estrogen. Here, we manipulated “menopause” status (ovary-intact and ovariectomized) in a mouse mammary tumor model to determine the extent to which ovarian status modulates: 1) tumor effects on estrogen concentrations and signaling in the brain, 2) tumor effects on estrogen-associated neurobiology and inflammation, and 3) the ability for tumor resection to resolve the effects of a tumor. As estrogens are anti-inflammatory and beneficial to cognitive and emotional behavior, we hypothesized that reduced circulating estradiol (E2) following ovariectomy exacerbates tumor-induced peripheral and central inflammation. Notably, we observed ovarian-dependent modulation on tumor-induced peripheral outcomes, including E2-dependent processes and, to a lesser degree, circulating inflammatory markers. In the brain, ovariectomy exacerbated neuroinflammatory markers in select brain regions and modulated E2-related neurobiology due to a tumor and/or resection. Overall, our data suggest that ovarian status has moderate implications for tumor-induced alterations in neuroendocrinology and neuroinflammation and mild effects on peripheral inflammatory outcomes in this murine mammary tumor model.
Fracture Prediction of Inconel Sheet Deformed under Different Stress States
Authors: Morchhale Ayush , Ural U Sachin , Arvikar Siddhant , Pandre Sandeep , Kotkunde Nitin , Singh Swadesh Kumar 
Published: April 2022

Abstract
The present work focuses on the prediction of onset of fracture for thin-walled metallic sheets used for forming complex shape and lightweight components for aerospace applications. The Lou-Huh ductile damage model has been used along with Barlat 1989 anisotropic yielding function for the prediction of fracture limits of Inconel alloy in different stress states, namely, pure shear, uniaxial tension, pure strain and equi-biaxial tension. Nine different types of specimens have been deformed experimentally till the occurrence of fracture using simple tensile test and stretch forming setup. The principal stresses at the onset of fracture have been determined experimentally and numerically and plotted in the major-minor strain space and, further, have been transformed into the effective plastic strain to fracture vs. stress triaxiality space. The fracture sites in the numerical simulations have been found to be consistent with the experimental results. Cockcroft-Latham, Brozzo, Oh and Rice-Tracey model have also been used for determining the fracture locus but the Lou–Huh model has only been found to accurately predict the fracture limit in all the stress states for a wide range of stress triaxiality as it considers the effect of shear-linkage of voids.
The genetic and epigenetic regulation of CD55 and its pathway analysis in colon cancer
Authors: Liu Jiawei , Fu Ning , Wu Hongjiao , Jin Ye , Li Ang , Song Qinqin , Ji Shanshan , Zhang Zhi , Zhang Xuemei 
Published: April 2022

Abstract
Background
CD55 played an important role in the development of colon cancer. This study aims to evaluate the expression of CD55 in colon cancer and discover how it was regulated by transcriptional factor and miRNA.
Methods
The expression of CD55 was explored by Tumor Immune Estimation Resource2.0 (TIMER 2.0), Human Protein Altas (HPA), and UALCAN databases. TRANSFAC and Contra v3 programs were used to predict the potential binding transcription factors in the CD55 promoter. TargetScan and Starbase v2.0 were used to predict potential binding ability of miRNAs to the 3’ untranslated region (3’UTR) of CD55. SurvivalMeth was used to explore the differentially methylated sites of CD55 promoter in colon cancer. Dual-luciferase reporter assay was performed to determine the targeting relationship of TFCP2, NF-κB, or miR-27a-3p with CD55. CD55 related genes was explored by constructing a protein-protein interaction (PPI) network. The pathway analysis was performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis.
Results
CD55 was significantly highly expressed in colon cancer tissues when compared with that in adjacent normal colon tissues. Dual luciferase reporter assays for CD55 promoter region showed that after knocking down TFCP2, the relative luciferase activity was decreased by 21% (P < 0.05); after inhibiting NF-κB, the relative luciferase activity was decreased by 70% (P < 0.01). The reporter gene activity assay presented that luciferase activity affected by 3’UTR of CD55 was decreased by 46% in the presence of targeting miR-27a-3p (P < 0.001). PPI network assay revealed a set of CD55-related genes, which including CFP, CFB, C4A, C4B, C5AR1, C3, C3AR1, C2, EGF, LCK, CD59, PIGA, PGAP1, ICAM1, EMR2 and CD97. GO and KEGG analysis revealed that the target genes occurred more frequently in immune-related pathways.
Conclusion
Our results indicated that CD55 was regulated by TFCP2, NF-κB, miR-27a-3p and several immune-related genes, which in turn affects colon cancer.
An Adaptive Interactional Technique Based on the Five-Dimensional Emotional Model for Webpage Interface Design
Authors: Zhang Hanzhong , Yin Jibin , Qin Haonan 
Published: April 2022

Abstract
The five-dimensional emotional model has been proposed for the recognition of emotions. This paper presents an adaptive emotional technique for webpage interface design based on it, which adaptively adjusts the presentation of various elements of web user interfaces by recognizing the changes in a user’s facial expression. The adjustments in the web interface, in turn, affect the user’s emotions. An experiment was designed and conducted to examine the relationship between interface elements and users’ emotional dimensions. The experimental results showed that there was a significant correlation between the layouts of the web user interfaces and the users’ emotions. Using a multi-layer perceptron, a neural network model was then trained to transform the values of the users’ emotional dimensions and interface element values, and a quantifiable transformation relationship between the webpage elements and users’ emotions was obtained. Finally, design guidelines based on our proposed adaptive technique are provided for web user interfaces.
Quality of life and the impact from sociodemographic and clinical parameters in Chinese chronic lymphocytic leukemia patients: an observational cohort study
Authors: Li Heng , Lian Qiaoping , Luo Ming , Wang Peilong , Yin Le , Zhang Donghua , Wang Jun , Li Wei , Wu Cuicui , Deng Shibin , Wu Daxing , Peng Hongling 
Published: April 2022

Abstract
Background: Relatively few studies have reported quality of life (QoL) and psychosocial distress in patients with chronic lymphocytic leukemia (CLL). This study aims to assess the QoL and psychological distress and the impact from sociodemographic and clinical parameters in the Chinese context.Methods: Taking advantage of the European Organization for Research and Treatment of Cancer (EORTC) QLQ-C30 questionnaire with QLQ-CLL17 module and DASS-21 questionnaire, a total of 50 Chinese patients with CLL completed self-reported questionnaires, assessing the level of depression, anxiety, stress and QoL.Results: A significantly higher proportion of patients in the active-treatment group had impaired physical function than the “watch and wait” group (90% vs. 65%, P=0.03). Patients with active treatment reported a significantly lower QoL in the role functioning subscale (84.44±25.50 vs. 95.83±9.17, P=0.031) and cognitive functioning subscale (76.67±18.88 vs. 89.17±12.42, P=0.007). The symptom scales had a negative impact on the functioning subscale. A dramatically high proportion of patients reported psychological distress. With regard to sociodemographic parameters, gender, age, education background and income had certain impact on functional subscales. 5 patients retested the questionnaires after median 8（2-18）months. Compared to the initial assessment, they scored significantly worse on the emotional scales (P=0.0237).Conclusions: There are unique correlates of QoL and disease status among Chinese CLL patients. This finding illuminates individual differences that may be associated with QoL. Therefore, psychosocial interventions and survivorship care plans should be made in the management of CLL survivors.
Impact of preoperative white blood cell count on long-term outcome in patients undergoing surgery for colorectal cancer: A retrospective observational study of Chinese patients
Authors: Wang Bei , Li Lihong , Wang Shilai , Zhou Changming , Sun Zhirong , Weng Meilin 
Published: April 2022

Abstract
Purpose: The correlation between preoperative white blood cell (WBC) count and the outcome in patients who underwent surgery for colorectal cancer (CRC) was evaluated in present study.Patients and methods: A total of 8121 Chinese patients at Fudan University Shanghai Cancer Center (FUSCC) undergoing colectomy, Dixon’s or Mile’s, for CRC from January 2008 to December 2014 were enrolled in present study. Based on that the WBC optimal cut-off value was ﻿7*109, the patients were divided into two groups, the moderate leukocytosis group (the leukocytosis group) and non-leukocytosis group. The impact of preoperative WBC count on disease-free survival (DFS) and overall survival (OS) was investigated using the Kaplan-Meier method and Cox proportional hazard models.Results: Moderate leukocytosis (≥﻿7*109) was recognized as a prognostic factor for survival independently. Moderate leukocytosis was significantly associated with male sex (P﻿＜0.001), advanced T stage (P﻿＜0.001), TNM stage (P﻿＜0.001), and no preoperative chemotherapy (P﻿＜0.001). Moderate leukocytosis group was corelated with poorer overall survival (OS) (P﻿＜0.001) and disease-free survival (DFS) (P=0.024) than non-leukocytosis group. In the multivariate analysis, the moderate leukocytosis group had a 19.6% higher risk in OS (HR=1.196, 95CI%:1.088-1.314, P<0.001) and had a 13.1% higher risk in DFS (HR=1.131, 95CI%:1.016-1.259, P=0.024) than the non-leukocytosis group. We found that the association with preoperative moderate leukocytosis and poorer OS (P﻿＜0.001) or DFS (P﻿=0.001) in patients with no preoperative chemotherapy in subgroup analysis.Conclusions: Preoperative moderate leukocytosis is associated with shorter OS, DFS indicating poor outcome in CRC patients after surgery.
Realgar-induced neurotoxicity: Crosstalk between autophagic flux and the p62-NRF2 feedback loop mediates p62 accumulation to promote apoptosis
Authors: Feng Rui , Liu Jieyu , Yang Zhao , Yao Tiantian , Ye Ping , Li Xiuhan , Zhang Jiaxin , Jiang Hong 
Published: April 2022

Abstract
Realgar is a traditional Chinese medicine that contains arsenic. It has been reported that the abuse of medicine containing realgar has potential central nervous system toxicity, but the toxicity mechanism has not been elucidated. In this study, we established realgar exposure model in vivo and selected DMA, the end product of realgar metabolism, to treat SH-SY5Y cells in vitro. Many assays including behavioral, analytical chemistry, and molecular biology were used to elucidate the roles of autophagic flux and p62-NRF2 feedback loop in realgar-induced neurotoxicity. The results showed that arsenic could accumulate in the brain, causing cognitive impairment and anxiety-like behavior. Realgar impairs the ultrastructure of neurons, promotes apoptosis, perturbs autophagic flux homeostasis, amplifies the p62-NRF2 feedback loop, and leads to p62 accumulation. Further analysis showed that realgar activates JNK/c-Jun, promotes the formation of Beclin1/Vps34 complex, induces autophagy, and recruits p62. Meanwhile, realgar inhibits the activities of CTSB and CTSD, and changes the acidic of lysosomes, resulting in the inhibition of p62 degradation, and then accumulation. Moreover, the amplified p62-NRF2 feedback loop is also involved in the accumulation of p62. Its accumulation promotes neuronal apoptosis by up-regulating the expression levels of Bax and cleaved-caspase-9, resulting in neurotoxicity. Taken together, these data suggest that realgar can perturb the cross-talk between autophagic flux and the p62-NRF2 feedback loop to mediate p62 accumulation, promote apoptosis, and induce neurotoxicity.
Anti-NMDAR encephalitis with euthyroid sick syndrome and lateral ventricle lesions predicts a poor prognosis
Authors: Wang Gong , Liu Juan , Yue Chengsong , Zhang Xi , Wang Hongyu , Luo Xue , Yang Xuesen 
Published: April 2022

Abstract
Background
The aim of this study was to clarify the role of thyroid dysfunction in anti-NMDAR encephalitis (ANE) patients.
Methods
The clinical and laboratory characteristics of 42 ANE patients were analyzed, including 24 ANE patients with normal thyroid function and 18 ANE patients with thyroid dysfunction (12 ANE patients with euthyroid sick syndrome (ESS); 6 ANE patients with thyroid dysfunction but not ESS). Cranial magnetic resonance imaging (MRI) findings, cerebrospinal fluid (CSF) parameters, clinical symptoms, antibodies, and admission and discharge modified Rankin scale (mRS) scores were evaluated.
Results
ANE patients with thyroid dysfunction had more serious consciousness disorders (p = 0.008), motor disorders (p = 0.005) and status epilepticus than those without thyroid dysfunction (p = 0.007). The conditions of ANE patients with ESS were more serious than those of ANE patients without ESS in terms of language disorders (p = 0.035), status epilepticus (p = 0.001), motor disorders (p < 0.007) and consciousness disorders (p = 0.001). Moreover, the admission mRS scores of ANE patients with ESS were higher than those of ANE patients without ESS (p = 0.030). ANE patients with ESS with lateral ventricle lesions (p = 0.017) had higher discharge mRS scores than those without lateral ventricle lesions (p = 0.045).
Conclusion
The conditions of ANE patients with thyroid dysfunction were more serious than those of ANE patients without thyroid dysfunction. The conditions of ANE patients with ESS were more serious than those of ANE patients without ESS, and these patients had poorer prognoses. The lateral ventricles were not common sites of lesions in autoimmune encephalitis patients, but they might predict a worse prognosis in ANE patients with ESS. Such patients should be given more attention. However, interventions for ESS are still controversial. Prospective randomized controlled trials should be performed to further explore the possible mechanisms of ESS in ANE.
Predictors of Sick Leave among Healthcare Workers in a Lebanese Tertiary Medical Center: A Retrospective Analysis
Authors: Sakr Carine , Fakih Lina , Musharrafieh Umayya , Makki Maha , Doudakian Rita , Tamim Hani , Redlich Carrie , Slade Martin , Rahme Diana 
Published: April 2022

Abstract
Background:Previous studies have shown that demographic, social, and work-related factors can influence sick-leave rates among healthcare workers. In this multivariable study, we evaluated potential predictors of sick leaves among the employees of a tertiary medical center in Lebanon between the years 2015 and 2018.Methods: This study was a retrospective analysis of sick leave records of the population of healthcare workers who took sick leave between 2015 and 2018. The records included a large sample size of 2,850 employees. Descriptive statistics were run, and the number of sick leave episodes was stratified by sick leave diagnosis. Bivariate analysis was done to investigate the association of the outcome with all predictors. To identify potential predictors of sick leave episodes, a multiple linear regression was conducted, and a p-value<0.05 was considered statistically significant.Results:Infectious diseases and musculoskeletal disorders diagnoses accounted for the highest number of sick leaves throughout the study period. All diagnoses had a short duration of sick leave days, with the mean duration of all diagnoses being 2.60 days. As for predictors of number of sick leave episodes, results from multiple regression analysis showed that the female gender, older age, marital status, lower job grade, job position, smoking status, and some medical conditions (namely gastrointestinal problems, respiratory problems, kidney disease, headache, back pain, and anxiety) were all significant predictors of taking more sick-leave episodes in the study population. Conclusion:Analyzing sick leave is a complex process with multifactorial determinants, and our study found a significant association between multiple demographics, work related, and health related predictors with the number of sick leave episodes. These findings call for further research to evaluate how interventions such as smoking cessation programs, safe patient handling programs, and employee assistant programs could impact sick leave rates among healthcare workers. Such interventions would guide policies for healthier, safer, and more productive workplaces.
Relation between the number of the older adults patients hospitalized for pneumonia and air particulate matter exposure: a time-stratified case-crossover study in multiple cities
Authors: Lin Chengwei , Jiang Wanyanhan , Gao Xi , He Yi , Li Jia , Zhou Chengchao , Yang Lian 
Published: April 2022

Abstract
Background: Many previous studies have already proved the relationship between air pollutants and respiratory diseases. However, limited studies have so far assessed the impacts of air particulate matter exposure on the older adults patients with pneumonia. This study aims to reveal the impacts of short-term exposure to air particulate matter on the daily number of the older adults patients hospitalized due to pneumonia and calculate the economic costs attributable to this exposure.Methods: A time-stratified case-crossover study design was adopted. Hospitalization data were collected from hospitals above county levels from 9 cities in Sichuan Province, China from January 1, 2018 to December 31, 2019. A sample of 43,746 pneumonia patients aged over 65 was retrieved. After adjusting for the influence of temperature and humidity, we analyzed the relationships between the daily number of the older adults pneumonia inpatients and the levels of air particulate matters (PM2.5, and PM10) and their relevant economic burdens. Then, stratification analyses were conducted based on patient age, gender, season, and whether the patient has comorbidity.Results: Short-term exposure to air particulate matter pollution was correlated with the increase of the older adults pneumonia inpatients. The single pollutant model showed that PM2.5 and PM10 had the greatest impact on the number of the older adults pneumonia inpatients at lag7 and lag4, respectively. For every 10 μg/m3 increase in PM2.5 and PM10 concentrations, the daily number of the older adults pneumonia inpatients increased by 1.5% (95% CI: 1.010-1.021) and 1.0% (95% CI: 1.006-1.014), respectively. The 65-79 year-olds were more susceptible to air particulate pollutants (P < 0.05). During the study period, the total hospitalization costs and out-of-pocket expenses attributable to PM2.5 and PM10 exposure were 44.60 million CNY and 16.03 million CNY, respectively, with PM2.5 being the primary influencing factor.Conclusion: Short-term exposure to air particulate pollution increased the risks of hospitalization for pneumonia in the 65 plus age group, increasing economic burdens on the society and involved families. These findings can further support the policies aiming to reduce the number of the older adults pneumonia inpatients by controlling air particulate pollutant concentrations.
Role of the Specialized Proresolving Mediator in Amyotrophic lateral sclerosis
Authors: Wei Xiao-jing , Li Xi-xi , Sun Hui , Qiu Ru-qing , Yu Xue-fan 
Published: April 2022

Abstract
Background
Amyotrophic Lateral Sclerosis (ALS) is a devastating progressive neurodegenerative disease that affects motor neurons, leading to a relentless paralysis of skeletal muscles and eventual respiratory failure. Chronic inflammation is one of the hallmarks of ALS and may contribute to motoneurons degeneration. Accumulating evidence suggests that chronic inflammation resulting from insufficient resolution, a process operated by specialized pro-resolving mediators (SPMs). However, whether resolution of inflammation is impaired in ALS is not known. The objective of this study was to investigate the levels of SPMs in the serum and cerebrospinal fluid (CSF) of ALS patients, and to explore the roles of SPMs in clinical features of ALS.
Methods
Fourty-one patients with ALS, and 73 patients with non-inflammatory neurological diseases were enrolled in this study. Pro-resolving mediators including resolvin D1 (RvD1), maresin 1 (MaR1) and lipoxinA4(LxA4) levels were analyzed using enzyme-linked immunosorbent assay. Pro- and anti-inflammatory cytokines levels were analyzed by cytometric beads array (CBA).
Findings:
Our results showed serum RvD1 levels were decreased, whereas CSF RvD1 levels were significantly increased in ALS patients compared to controls. Serum RvD1 was also negatively correlated to disease progression rate (DPR), and positively correlated with forced vital capacity (FVC).
Conclusions
The present preliminary study allows hypothesizing impaired resolution of inflammation in patients with ALS, confirming the emerging role of bioactive lipids in this disease.
Effects of Lactobacillus plantarum inoculation on the quality and bacterial community of whole-crop corn silage at different harvest stages
Authors: Dong Jianan , Li Songze , Chen Xue , Sun Zhe , Sun Yue , Zhen Yuguo , Qin Guixin , Wang Tao , Demelash Natnael , Zhang Xuefeng 
Published: April 2022

Abstract
Background: Silage is achieved by anaerobic fermentation of lactic acid bacteria. However, harvest stage and Lactobacillus plantarum (L. plantarum) content affect the changes of microbial community in silage, which play an important role in silage quality and mycotoxin levels. The current study evaluated the effects exerted by the application of L. plantarum on fermentation, mycotoxins and bacterial community of whole-crop corn silage at different harvest stages. The fresh whole plant corn from the same area was harvested during the milk stage and the dough stage, following which from each harvest stage was subjected to four L. plantarum treatments: 0 cfu/kg; 1×109 cfu/kg; 1×1010 cfu/kg; and 1×1011 cfu/kg in a 2×4 factorial experimental design. Samples were collected on days 0, 7, 14, 21 and 42. Subsequently, nutrition parameters, fermentation parameters, mycotoxin levels and bacterial community were determined. Results: Prolonging the harvest stage resulted in an increase in the levels of starch and dry matter as well as deoxynivalenol in corn silage. Addition of 1×1010 cfu/kg and 1×1011 cfu/kg of L. plantarum applied after 42 d of ensilage reduced dry matter loss, which significantly lowered deoxynivalenol concentration in corn silage at the dough stage (P < 0.01). The pH of corn silage decreased, whereas the lactic acid concentration increased after ensiling. The effects exerted by the interaction (harvest stage × dose of L. plantarum ) on pH on day 21 as well as on fermentation and lactic acid on days 21 and 42 were significant (P < 0.05). Addition of 1×1010 cfu/kg of L. plantarum at the dough stage significantly increased the relative abundance of Firmicutes while decreasing that of Bacteroidetes, compared to when L. plantarum was not added (P < 0.05). Conclusion: Adding L. plantarum improved the fermentation quality and bacterial community of silage, which reduced part of mycotoxin content and relative abundance of harmful bacteria. And adding L. plantarum in dough stage resulted in greater improvements to mycotoxin content and bacterial community.