bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of ATG12–ATG5-ATG16L1 autophagy E3 complex on the ability of LC3/GABARAP proteins to induce vesicle tethering and fusion
Authors: Iriondo Marina N. , Etxaniz Asier , Varela Yaiza R. , Ballesteros Uxue , Lázaro Melisa , Valle Mikel , Fracchiolla Dorotea , Martens Sascha , Montes L. Ruth , Goni Felix M , Alonso Alicia 
Published: August 2022

Abstract
In macroautophagy, the autophagosome (AP) engulfs portions of cytoplasm to allow their lysosomal degradation. AP formation in humans requires the concerted action of the ATG12 and LC3/GABARAP conjugation systems. The ATG12–ATG5-ATG16L1 (E3) complex acts as a ubiquitin-like E3 ligase enzyme, promoting LC3/GABARAP protein anchoring to the AP membrane. The role of the various proteins in the AP expansion process is still unclear, in part because there are no studies comparing LC3/GABARAP-family member roles under the same conditions, and also because the full human E3 complex was only recently available. In the present study, the lipidation of six members of the LC3/GABARAP family has been reconstituted in the presence and absence of E3, and the mechanisms by which E3 and LC3/GABARAP proteins participate in vesicle tethering and fusion have been investigated. In the absence of E3, GABARAP and GABARAPL1 showed the highest activities. Differences found within LC3/GABARAP proteins suggest the existence of a lipidation threshold, lower for the GABARAP subfamily, as a requisite for tethering and inter-vesicular lipid mixing. E3 increases and speeds up lipidation and LC3/GABARAP-promoted tethering. However E3 hampers LC3/GABARAP capacity to induce inter-vesicular lipid mixing or subsequent fusion, presumably through formation of a rigid scaffold on the vesicle surface. Our results suggest a model of AP expansion in which the growing regions would be areas where the LC3/GABARAP proteins involved should be susceptible to lipidation in the absence of E3, or else a regulatory mechanism would allow vesicle incorporation and phagophore growth when E3 is present.
Comparison between azacitidine and decitabine as front-line therapy in elderly treatment naïve Acute Myeloid Leukemia not eligible for intensive chemotherapy
Authors: Maurillo Luca , Spagnoli Alessandra , Candoni Anna , Papayannidis Cristina , Borlenghi Erika , Lazzarotto Davide , Fianchi Luana , Sciumè Maria Rita , Zannier Maria Elena , Buccisano Francesco , Principe Maria Ilaria Del , Mancini Valentina , Breccia Massimo , Fanin Renato , Todisco Elisabetta , Lunghi Monia , Palmieri Raffaele , Fracchiolla Nicola , Musto Pellegrino , Rossi Giuseppe , Venditti Adriano 
Published: August 2022

Abstract
We compared the efficacy of azacitidine (AZA) and decitabine (DEC) in elderly patients with untreated AML, diagnosed according to WHO criteria. In the two groups, we evaluated complete remission (CR), overall survival (OS) and disease free survival (DFS). The AZA and DEC groups included 139 and 186 patients, respectively. To minimize the effects of treatment selection bias, adjustments were made using the propensity-score matching method, which yielded 136 patient pairs. In the AZA and DEC cohort, median age was 75 years in both, (IQR, 71–78 and 71–77), median WBCc at treatment onset 2.5x109/L (IQR, 1.6–5.8 ) and 2.9x 109/L (IQR, 1.5–8.1), median bone marrow (BM) blast count 30% (IQR, 24–41%) and 49% (IQR, 30–67%), 59 (43%) and 63 (46%) patients had a secondary AML, respectively. Karyotype was evaluable in 115 and 120 patients: 80 (59%) and 87 (64%) had intermediate-risk, 35 (26%) and 33 (24%) an adverse risk karyotype, respectively. Median number of cycles delivered was 6 (IQR, 3.0–11.0) and 4 (IQR, 2.0–9.0), CR rate was 24% vs 29%, median OS and 2-year OS rates 11.3 (95% CI 9.5–13.8) vs 12.0 (95% CI 7.1–16.5) months and 20% vs 24%, respectively. No differences in CR and OS were found within the following subgroup: intermediate- and adverse-risk cytogenetic, frequency of WBCc at treatment ≥ 5x10^9L and < 5x10^9/L, de novo and secondary AML, BM blast count < and ≥ 30%. Median DFS for AZA and DEC treated patients was 9.2 vs 12 months, respectively. Our analysis indicates similar outcomes with AZA compared to DEC.
Evaluation of the coordination between China’s marine ecological environment and marine economic development
Authors: Cai Ling , Liang Junlang , Guo Zhouhua , Ouyang Yurong , Yang Ming , Dai Juanjuan 
Published: August 2022

Abstract
Since the 21st century, rapid development of marine economies has contributed considerably to economic growth. The problem of coordinated development of marine ecological environmental protection and economies have become increasingly prominent. This study establishes a set of index evaluation systems for coordinated development of marine ecology and economies and uses the Analytic Hierarchy Process to analyze the coordination of marine ecology and marine economic development in China. The results show that more attention has been paid to the protection of marine ecological environment during marine economic development in China since the year 2012; Furthermore, the coordination of marine economic development and marine ecological protection in coastal provinces was analyzed. Results show that Shanghai and Shandong Province better coordinate marine economic development and marine ecological protection in 2016. The results of the index evaluation system suggest that China marine ecological protection should further increase the protection of marine ecological environments by preventing and controlling marine pollution and carrying out ecological restoration.
Fully Verifiable Outsourcing of Single and Composite Modular Exponentiation with Single Untrusted Cloud Server
Authors: Torki Omid , Mala Hamid , Ashouri-Talouki Maede , Alkhzaimi Hoda 
Published: August 2022

Abstract
Modular exponentiation is considered one of the most widely used, yet costly operations in public-key cryptography, especially for resource-constrained devices in a peer-to-peer networks such as net of IoT or sensor devices. However, it can be computed at a relatively low cost with the help of outsourcing. The first contribution of this paper is investigating the security of the Su et al.’s scheme which, our first attack confirms that the verifiability of this scheme is less than the authors’ claim and our second attack reveals that a malicious server is able to forge user signature. The second contribution of this paper is investigating the verifiability of Ding’s scheme, a scheme previously claimed to provide high verifiability, and we shows that this scheme is not verifiable. The third contribution is centered around proposing two practical and secure outsourcing schemes for outsourc-ing computations of single and composite modular exponentiation, in the single-server model. It is worth noting that both of our proposed schemes offer higher efficiency and security than the previous schemes as their verifiability are equal to one, while the recorded verifiability of the previous schemes decreases by the number of exponentiations utilized.
A Novel Method to Develop Nanocomposite Bimodal Foams Containing Expandable Polymeric Microballoons: Microstructural Characteristics
Authors: Mohammadian Zahra , Azdast Taher 
Published: August 2022

Abstract
This study aims to develop a novel technique in manufacturing nanocomposite bimodal foams containing expandable polymeric microballoons. Low density polyethylene (LDPE) syntactic foams were prepared via injection molding process, afterwards, a batch refoaming method was utilized to create bimodal structure. The effects of microballoon and nanoclay content and foaming time and temperature on microstructure and physical properties of foams were investigated. The results revealed that refoaming leads to a considerable decrease in density due to nucleation of microcells along with re-expansion of microballoons, as well as CO2 diffusion in voids between the matrix and microballoon surfaces. Microballoon content has no significant effect on cell size of bimodal foams, while a great growth in cell density was observed as its content increased. Results also indicated that at low and high foaming process parameters, melt strength and gas loss are the overcoming phenomena, respectively leading to an optimal processing temperature and time.
Efficacy and safety of double balloon catheter and dinoprostone for labor induction in multipara at term
Authors: Yuan Lu , Peng Jing , Yang Lijun , Zhao Yun 
Published: August 2022

Abstract
Background: The aim of this study was to comparatively assess the efficacy and safety of double balloon catheter (DBC) and dinoprostone and as labor-inducing agents for multipara at term. Methods: A retrospective cohort study was conducted among multipara at term with a Bishop score <6 who needed planned labor induction from January 1, 2020, to December 30, 2020 in Maternal and Child Health Hospital of Hubei province, Tongji Medical College, Huazhong University of Science and Technology. They were divided into DBC group and dinoprostone group respectively according to the method of labor induction. Baseline maternal data and perinatal outcomes were recorded for statistical analysis. Total vaginal delivery rate, rate of vaginal delivery within 24 h, rate of uterine hyperstimulation combined with abnormal fetal heart rate(FHR) and rate of fetal distress were regarded as the primary outcome variables. The diffrerence between groups was considered statistically significant when p value <0.05. Results: A total of 202 pregnant women was included for analysis (95 women in DBC group vs 107 women in dinoprostone group). There were no significant differences in total vaginal delivery rate and rate of vaginal delivery within 24 h between groups (93.7% vs 86.9% and 75.8% vs 71%, p＞0.05). Uterine hyperstimulation combined with abnormal FHR occurred exclusively in dinoprostone group (0% vs 5.6%, p＜0.05). The rate of amniotic fluid fecal staining and prenatal fever in dinoprostone group was higher than in DBC group (p＜0.05). There was no significant difference in the rate of fetal distress between in the two groups (4.2% vs 10.3%, p＞0.05). Both groups were similar in neonatal outcomes including birth weight, neonatal asphyxia and neonatal intensive care unit admission (NICU). Conclusions: DBC and dinoprostone seem to be equally effective in labor induction for multipara at term, and DBC seems to be safer than dinoprostone during induction in multipara at term as it causes lower rate of uterine hyperstimulation combined with abnormal FHR.
Iontronic pressure sensor with ultra-board linear range and high sensitivity enabled by laser-induced gradient pyramidal structures
Authors: Yang Ruoxi , Dutta Ankan , Li Bowen , Tiwari Naveen , Zhang Wanqing , Niu Zhenyuan , Gao Yuyan , Erdely Daniel , Xin Xin , Li Tiejun , Cheng Huanyu 
Published: August 2022

Abstract
Despite the extensive developments of flexible capacitive pressure sensors, it is still elusive to simultaneously achieve excellent linearity over a broad pressure range, high sensitivity, and ultrahigh pressure resolution under large pressure preloads at low cost. This work presents a facile and low-cost fabrication method to integrate an ultrathin ionic layer with gradient microstructures with programmable profiles and heights created by a simple CO2 laser. The coupled electrical and mechanical simulations provide a route to optimize the design of iontronic pressure sensors based on the electric double layer to address the existing challenges for significantly improved pressure sensing performance. The resulting optimized sensor exhibits a high sensitivity of 33 kPa− 1 over an ultra-board linear sensing range of 1700 kPa, an ultralow detection limit of 0.36 Pa, and a pressure resolution of 0.00725% under ultrahigh pressure of 2000 kPa. Taken together with a rapid response/recovery time of 4/16 ms and excellent repeatability over 4,500 cycles, the sensor has been applied to subtle pulse detection from the fingertip, interactive control on the robotic hand, and a smart weight scale/chair with ultrahigh pressure resolution. The simple fabrication approaches and design toolkit from this work can also be leveraged to easily tune the pressure sensor performance for varying target applications and open up the opportunities to create other iontronic sensors for the next-generation flexible devices.
Deep low-frequency earthquake activity associated with the 2018 eruptions in the Kirishima volcano group, Japan
Authors: Kurihara Ryo , Kato Aitaro 
Published: August 2022

Abstract
Deep low-frequency (DLF) earthquakes have occurred at depths of 10–30 km in the Kirishima volcano Group, Japan. Here we investigate the DLF earthquake activity that was associated with the 2018 eruptions, compare these DLF earthquakes with those associated with the 2011 eruptions, and provide inferences on magmatic fluid ascension during these two eruptions. We apply a new matched-filter method to the continuous waveform data from the 2017–2018 period to comprehensively detect the DLF earthquake activity surrounding the 2018 eruptions. This new method can detect microearthquakes using a single seismic station based on an index that is computed as the product of mutual information and the correlation coefficient to measure the similarity between the template and target waveforms. We perform the same analysis using the 2010–2011 waveform data for comparison with the DLF earthquake activity associated with the 2011 eruptions. We detect 75 DLF earthquakes at approximately 25 km depth during the 2017–2018 period, whereas we detect 1302 DLF earthquakes at similar depths during the 2010–2011 period. Although the number of detected 2017–2018 events is small, we identify two swarms of DLF earthquake activity in March and July 2017. The March 2017 swarm coincides with the appearance of mud pots and jet fumaroles at the surface, and the July 2017 swarm coincides with the initiation of crustal deformation, which indicates the inflation of a deep magma reservoir. Furthermore, the occurrence rate of DLF earthquakes increased slightly after the March 2018 eruptions. Although the occurrence rate of DLF earthquakes associated with the 2018 eruptions was much lower than that associated with the 2011 eruptions, the slight increase in DLF earthquakes during the 2018 eruptions implies a connection between the deep magmatic fluid ascension and shallow volcanic unrest in 2018, which is similar to that observed during the 2011 eruptions. Such a close temporal relationship between the DLF earthquakes and surface volcanic activity suggests that the pressure disturbance within volcanic conduits propagates rapidly from depth.
Investigation into the potential of using UV-treated sporulated oocysts of Eimeria tenella as a local solution to immunization of chickens against caecal coccidiosis
Authors: Samir Djemai , Mekroud Abdeslam , Hide Geoff , Khelifi Daoudi , Bellil Inès 
Published: August 2022

Abstract
In this study, we aim to evaluate the immune response of chickens to UV-treated sporulated oocysts as a means of protection against caecal coccidiosis caused by field strains of Eimeria tenella. Two groups of chicks were immunized using prepared UV-treated oocysts of E. tenella and challenged at day 20 post hatching. The first group was immunized only once at day 1 post hatching, the second group was immunized twice (day 1 and day 8 post hatching). Two non-immunized control groups were used: the first group was challenged with E. tenella, while the second group remained uninfected. The effectiveness of immunization on production and animal health was evaluated by the following criteria: body weight, feed conversion ratio, blood in faeces, mortality, lesion scores and oocyst output. The two immunized groups showed a significantly better performance in body weight, weight gain and lesion scores than the non-immunized group. However, all three groups performed significantly worse than the unchallenged group. The mortality of the non-immunized infected group was high (70%) while mortality in both immunized and unchallenged groups of chickens was significantly lower (range 2.2 to 4.4%) than the infected group (p<0.05). The production of oocysts in faeces, post-infection, was significantly higher in the non-immunized group compared to the immunized group (p<0.05) and both were significantly higher than the uninfected group (p<0.05). In conclusion, immunization by prepared UV-irradiated oocysts is effective in stimulating at least a partial protective immunity in immunized chickens against caecal coccidiosis.
Research on Stress Performance of Universally Wrapped Assembled Joints of Lattice Wind Power Tower with Concrete-filled Steel Tube
Authors: Wen Yang , Liu Zhen , Xiong Wen 
Published: August 2022

Abstract
With the control parameters of the wall thickness of the ball table and the thickness of the pressure plate of the ball table, four universal wrapped assembled joint models were designed for the research of stress performance via static loading test. The failure mode of the joint, the axial force-displacement curve of the web member, the equivalent stress distribution of the ball table and the pressure plate of the ball table were analyzed, and the ABQAUS finite element software was used for the analysis of parameter expansion of the specimen. The research results show that the failure modes of universally wrapped assembled joints can be divided into the buckling failure of the ball table and the strength failure of the material. The wall thickness of the ball table is the key parameter affecting the failure mode of the joints, and the thickness of the pressure plate of the ball table is the crucial parameter influencing the ultimate bearing capacity of the joints. The maximum equivalent stress of the pressure plate and the ball table at compression zone for all the joints is occurred at the side squeezed by the bolt ball, and the growth rate of the equivalent stress for the ball table at compression zone is more uniform than that at tension zone. According to the finite element analysis results, when the wall thickness of the ball table is greater than 7mm, the thickness of the pressure plate of the ball table is greater than 16mm, the growth rate of the ultimate bearing capacity of the joint is significantly decreased, therefore, it is recommended to use the wall thickness of 7mm and the pressure plate thickness of 16mm in the actual design of the project.
ABCC10-mediated Oxaliplatin resistance in colorectal cancer cells was alleviated by intense endoplasmic reticulum stress (ERS) / IRE1α
Authors: Liu Xiaohui , Chen Hong , Sun Haimei , Guo Xiaoxia , Wu Bo , Sun Tingyi , Ji Fengqing , Yang Shu , Zhou Deshan 
Published: August 2022

Abstract
Background
Oxaliplatin resistance is a challenge in treating colorectal cancer (CRC) patients, contributory to the failure in chemotherapy and the risks in relapse and metastasis. However, the mechanism of Oxaliplatin resistance has not been completely elucidated.
Methods
Microarray screening, western blot and qPCR on clinic CRC samples were conducted to select the target gene ABCC10 transporter. The Cancer Genome Atlas data was analyzed to figure out the correlation between the clinical manifestation and ABCC10 expression. ABCC10 knock-down in CRC cells was conducted to identify its role in the Oxaliplatin resistance. Cell counting kit-8 assay was conducted to identify the CRC cell viability and Oxaliplatin IC50. Flow cytometry was conducted to detect the cell apoptosis exposed to Oxaliplatin. The intracellular Oxaliplatin accumulation was measured by ultra-high performance liquid chromatography coupled to tandem mass spectrometry.
Results
CRC patients with higher ABCC10 were prone to relapse and metastasis. Differential ABCC10 expression in multiple CRC cell lines revealed a strong positive correlation between ABCC10 expression level and Oxaliplatin resistance. In ABCC10 knock-down CRC cells the Oxaliplatin sensitivity was evidently elevated due to an increase of intracellular Oxaliplatin accumulation resulted from the diminished drug efflux. To explore a strategy to block ABCC10 in CRC cells, we paid a special interest in the endoplasmic reticulum stress (ERS) / unfolded protein response (UPR) that plays a dual role in tumor development. We found that neither the inhibition of ERS nor the induction of mild ERS had anti-CRC effect. However, the CRC cell viability was profoundly decreased and the pro-apoptotic factor CHOP and apoptosis were increased by the induction of intense ERS. Significantly, the Oxaliplatin sensitivity of CRC cells was enhanced in response to the intense ERS, which was blocked by inhibiting IRE1α branch of UPR. Finally, we figured out that the intense ERS down-regulated ABCC10 expression via regulated IRE1-dependent decay activity.
Conclusion
Oxaliplatin was a substrate of ABCC10 efflux transporter. The intense ERS/IRE1α alleviated Oxaliplatin resistance through down-regulating ABCC10 in addition to inducing CHOP. We suggested that introduction of intense ERS/UPR could be a promising strategy to restore chemo-sensitivity when used in combination with Oxaliplatin or other chemotherapeutic drugs pumped out by ABCC10.
Feasibility, effectiveness and costs of a decision support intervention for consultees and legal representatives of adults lacking capacity to consent (CONSULT): protocol for a randomised Study Within a Trial
Authors: Shepherd Victoria , Wood Fiona , Gillies Katie , Martin Adam , O’Connell Abby , Hood Kerenza 
Published: August 2022

Abstract
Background
Randomised trials play a vital role in underpinning evidence-based care. However, trials involving adults with impaired capacity to consent raise a number of ethical and methodological challenges, leading to the frequent exclusion of this group from trials. This includes challenges around involving family members as alternative ‘proxy’ decision-makers. Family members are often given little information about their role as a consultee or legal representative. Some family members find making a decision about trial participation difficult and may experience an emotional and decisional burden as a result. Families have reported a need for greater support and guidance when making such decisions, leading to the development of a decision aid (‘Making decisions about research for others’) for family members acting as consultee/legal representative. The decision aid now requires evaluation to determine its effectiveness in supporting families to make more informed decisions.
Methods
This protocol describes a prospective, multi-centre, randomised-controlled Study Within a Trial (SWAT) to evaluate the effectiveness of the decision aid. The SWAT will initially be embedded in approximately five host trials. Participants will be randomised in a 1:1 ratio to either the intervention (decision aid alongside standard information about the host trial provided to consultees/legal representatives) or control (standard information alone). The primary outcome is the quality of proxy consent decision, assessed by the Combined Scale for Proxy Informed Consent Decisions (CONCORD). The SWAT design is informed by previous qualitative research. Initial feasibility will be explored in one host trial, followed by the main SWAT. An embedded process evaluation and economic evaluation will enable the SWAT findings to be contextualised and identify factors likely to affect implementation.
Discussion
This SWAT will generate the first evidence for recruitment interventions for trials involving adults lacking capacity to consent and add to knowledge about the use of decision support interventions in trial participation decisions. The SWAT will be embedded in a range of trials, and the heterogenous nature of the host trials, settings and populations involved will enable the intervention to be evaluated in a wide range of contexts. However, a pragmatic and flexible approach to conducting the SWAT is needed.
Trial Registration
The SWAT is registered as SWAT #159 with the Northern Ireland Hub for Trials Methodology Research SWAT repository (registered 09.08.2020). Each host trial will be registered on a clinical trials registry.