bluuebunny/crossref_metadata_2025_split huggingface dataset:
A single centre study from western India to evaluate the frequency of developing second and subsequent multiple primary malignancies among cancer survivors
Authors: Sharma Anjali , Sharma Ajay , Patni Sanjeev , Gupta Anil , Ledwani Naresh , Saini Shashikant , Patni Nidhi , Bapna Ajay , Somani Naresh , Sharma Mudit , Verma Shubham , Sharma Gulshan Kumar , Nunia Vandana 
Published: September 2022

Abstract
Aim
This study was designed to evaluate the frequency of developing second and subsequent primary cancers among cancer survivors.

Methods
We have retrospectively analyzed 121 multiple primary cancer patients treated at Bhagwan Mahavir Cancer Hospital and Research Centre, Jaipur, Rajasthan, India from the last 20 years. The survival analysis was performed by Kaplan-Meier methodology.

Results
The prevalence of multiple primary malignant tumors (MPMTs) was 1.51% (121/8000), with a male to female ratio of 1:1.42. In males most of the reported MPMTs were synchronous and most common first primary cancer cases were head & neck and lung whereas, in females most of the reported MPMTs were metachronous and most common first primary cancer cases were breast and gynaecological malignancies. Family history was reported in 15 cases. Maximum 33.05% patients received combined treatment of chemotherapy, radiotherapy and 91.73% (111/121) of patients with MPMTs were effectively followed up, 35 (28.92%) patients died and 5-year survival rate of the remaining 86 patients was 68.46%.

Conclusions
In the present observational study the most frequent sites of MPMTs in men were head & neck and lung whereas, in women breast and gynaecological sites were common. Therefore, careful monitoring and follow up are required for these patients.
Medical cannabis authorization patterns, safety, and associated effects in older adults
Authors: MacNair Laura , Kalaba Maja , Peters Erica N. , Feldner Matthew T. , Eglit Graham M. L. , Rapin Lucile , El Hage Cynthia , Prosk Erin , Ware Mark A. 
Published: September 2022

Abstract
Background
Use of medical cannabis is increasing among older adults. However, few investigations have examined cannabis use in this population.

Methods
We assessed the authorization patterns, safety, and effects of medical cannabis in a sub-analysis of 201 older adults (aged ≥ 65 years) who completed a 3-month follow-up during this observational study of patients who were legally authorized a medical cannabis product (N = 67). Cannabis authorization patterns, adverse events (AEs), Edmonton Symptom Assessment Scale-revised (ESAS-r), and Brief Pain Inventory Short Form (BPI-SF) data were collected.

Results
The most common symptoms for which medical cannabis was authorized were pain (159, 85.0%) and insomnia (9, 4.8%). At baseline and at the 3-month follow-up, cannabidiol (CBD)-dominant products were authorized most frequently (99, 54%), followed by balanced products (76, 42%), and then delta-9-tetrahydrocannabinol (THC)-dominant products (8, 4.4%). The most frequent AEs were dizziness (18.2%), nausea (9.1%), dry mouth (9.1%), and tinnitus (9.1%). Significant reductions in ESAS-r scores were observed over time in the domains of drowsiness (p = .013) and tiredness (p = .031), but not pain (p = .106) or well-being (p = .274). Significant reductions in BPI-SF scores over time were observed for worst pain (p = .010), average pain (p = .012), and overall pain severity (p = 0.009), but not pain right now (p = .052) or least pain (p = .141).

Conclusions
Overall, results suggest medical cannabis was safe, well-tolerated, and associated with clinically meaningful reductions in pain in this sample of older adults. However, the potential bias introduced by the high subject attrition rate means that all findings should be interpreted cautiously and confirmed by more rigorous studies.
Fire interval and post-fire climate effects on serotinous forest resilience
Authors: Agne Michelle C. , Fontaine Joseph B. , Enright Neal J. , Harvey Brian J. 
Published: September 2022

Abstract
Background
Climate change is eroding forest resilience to disturbance directly through warming climate and indirectly through increasing disturbance activity. Forests characterized by stand-replacing fire regimes and dominated by serotinous species are at risk when the inter-fire period is insufficient for canopy seed bank development and climate conditions for recruitment in the post-fire growing season are unsuitable. Although both factors are critical to serotinous forest persistence, their relative importance for post-fire regeneration in serotinous forests remains poorly understood. To assess the relative effects of each factor, we established plots in severely burned knobcone pine (Pinus attenuata Lemmon) forests in Oregon and California, USA, representing a range of past fire intervals (6 to 31+ years). Specifically, we evaluated effects of fire interval and pre-fire canopy seed bank (proxies for seed supply) and post-fire climate on three metrics of post-fire tree regeneration (seedling density, probability of self-replacement, percent population recovery).

Results
Seed supply consistently had the strongest effect on post-fire regeneration. Between 6- and 31-year fire intervals, post-fire seedling density increased from 1000 to 100,000 seedlings ha−1, while probability of self-replacement increased from ~ 0 to ~ 100% and percent population recovery increased from 20 to 2000% of the pre-fire population, respectively. Similarly, increasing the canopy seed bank by two orders of magnitude increased seedling density and percent population recovery by two orders and one order of magnitude, respectively, and increased the probability of self-replacement by > 50%. Greater post-fire climatic moisture deficit exacerbated the effect of seed supply; an additional 4–6 years between fires was required under high moisture stress conditions to reach similar regeneration levels as under low moisture stress conditions.

Conclusion
The overriding effect of seed supply—strongly driven by pre-fire stand age—on post-fire regeneration suggests that altered fire frequency (an indirect effect of climate change) will have a profound impact on serotinous forests. Although direct effects of hot and dry climate are lower in magnitude, they can alter forest recovery where seed supply nears a threshold. These findings reveal how fire interval and climate combine to determine changes in forest cover in the future, informing management and vulnerability mapping.
Screening for depressive symptoms in patients with rheumatoid arthritis: relationship with pain severity, disease activity, and sleep quality
Authors: Azzam Adel Ibrahim , Lamlom Mohamed , Khalifa Ahmed Mohamed 
Published: September 2022

Abstract
Background
Rheumatoid arthritis (RA) is an autoimmune systemic condition that primarily affects all synovial joints, eventually leading to deformity and clinical disability. Much progress has been made in the evaluation of inflammation and disease activity in recent years; however, other factors that can influence these patients’ quality of life, including depression, stress, fatigue, sleep problems, fibromyalgia, sexual activity, and obesity, are often not evaluated by rheumatologists. Our purpose was to explore depressive symptoms in patients with RA and determine how they connected to other aspects of the disease, including pain severity, disease activity, and sleep quality.

Results
A cross-sectional study including 1200 patients with RA was performed. Paints with RA were classified into two groups based on the presence or absence of depressive symptoms using the Beck Depression Inventory-II (BDI-II). Group 1 included patients with both RA and depressive symptoms of varying severity; group 2 included patients with RA but without depressive symptoms. The patients underwent clinical evaluation and application of the Pittsburgh Sleep Quality Index (PSQI), Health Assessment Questionnaire for pain (HAQ-pain), and the Multidimensional Assessment of Fatigue scale (MAF). RA disease activity was evaluated using the DAS28 score. Depressive symptoms of varying severity were prevalent in 96% of our patients with RA, of whom 43.3% had minimal depression, while 13.7% had severe depression. The RA group with depression had a longer duration of disease, prolonged morning stiffness, and high disease activity measured by the DAS28 score than patients with only RA. In RA patients with concomitant depression, pain, sleep, and fatigue scores were also worse.

Conclusions
The presence of depression among patients with RA was associated with worse DAS28, HAQ, PASQI, and fatigue scores. Screening and recognition of such psychosocial disorders may help patients achieve optimal disease control and a good outcome.
Flavonoids as dual inhibitors of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX): molecular docking and in vitro studies
Authors: Md Idris Muhd Hanis , Mohd Amin Siti Norhidayu , Mohd Amin Siti Norhidayah , Nyokat Nyotia , Khong Heng Yen , Selvaraj Manikandan , Zakaria Zainul Amiruddin , Shaameri Zurina , Hamzah Ahmad Sazali , Teh Lay Kek , Salleh Mohd Zaki 
Published: September 2022

Abstract
Background
Inflammation is known to involve in many pathological processes of different diseases, but the current therapy causes adverse effects. Thus, there is a great interest for the discovery of flavonoids as a valuable alternative to classical analgesic and anti-inflammatory agent with dual-inhibitory action, especially on both COX-2 and 5-LOX which can minimize or overcome this problem.

Results
In the present work, drug-likeness properties of the synthesized flavonoids via Lipinski’s Rule of Five were predicted using QikProp prior to evaluation of their COX and LOX inhibitory activities using enzyme assays. Subsequently, molecular docking was performed using GLIDE to analyse their binding behaviour. The results showed that all compounds obeyed the Lipinski’s Rule of Five. NPC6 and NPC7 had displayed better selectivity towards COX-2 as compared to Indomethacin with less than 50% inhibition against COX-1. In addition, these compounds also inhibited activity of 5-LOX. Their selectivity to COX-2 was due to the binding to hydrophobic region and extends to lobby region near the entrance of COX binding site forming hydrogen bond with Ser530. Interestingly, these compounds showed a similar binding mode as Zileuton in the active site of 5-LOX and formed hydrogen bond interaction with Ala424.

Conclusion
NPC6 and NPC7 had potential as dual inhibitor of COX-2 and 5-LOX. The scaffolds of these chemical entities are useful to be as lead compounds for the dual inhibition of COX-2 and 5-LOX.
Green synthesis of silver nanoparticles using dietary antioxidant rutin and its biological contour
Authors: Kubavat Kinjal , Trivedi Pooja , Ansari Hafsa , Kongor Anita , Panchal Manthan , Jain Vinod , Sindhav Gaurang 
Published: September 2022

AbstractBackgroundDietary and wholesome antioxidant rutin is considered advantageous due to its potential protective role for numerous diseases related to oxidative stress, high safety, cost-effectiveness, and extensive biological effects. The present study accounts for an expeditious method for the synthesis of silver nanoparticles (AgNPs) using rutin.ResultsThe presence of AgNPs was affirmed by UV–visible spectroscopy at 425 nm, and FESEM and zeta sizer analysis revealed the average size of the AgNPs 80–85 nm and 160 d.nm, respectively. Zeta potential measurements (− 30.3 mV) showed that the AgNPs have reasonably good stability. Element mapping analysis of the AgNPs was confirmed by XRD and AFM, while FTIR spectra of the AgNPs showed the existence of functional groups. In the DPPH assay, highest radical scavenging activity of AgNPs, 86.95 ± 01.60%, was confirmed. The interaction of AgNPs with CT-DNA and HS-DNA was studied spectrophotometrically, and the data display a shift in the respective spectra. Furthermore, interaction with pBR322 DNA,λDNA, CT-DNA, and HS-DNA was deliberated by a nicking assay that shows the physicochemical properties of AgNPs. Antibacterial activity was evaluated by the standard well-diffusion method againstEscherichia coliandStaphylococcus aureus,and cytotoxicity was assessed against human WBCs by MTT assay.ConclusionAs per this appraisal, it can be concluded that it is a cost-effective, simple, and eco-friendly tactic and such NPs are beneficial to improve therapeutics since the antioxidant, DNA interaction, antibacterial, and cytotoxic exploits offer a new horizon of euthenics.
Lower Blood Oxygen Saturation is Associated With Microvascular Complications in Individuals With Type 1 Diabetes
Authors: Laursen Jens Christian , Mizrak Hatice Isik , Kufaishi Huda , Hecquet Sofie Korsgaard , Stougaard Elisabeth Buur , Tougaard Ninna Hahn , Frimodt-Møller Marie , Hansen Tine Willum , Hansen Christian Stevns , Rossing Peter 
Published: September 2022

Abstract

Context
Blood oxygen saturation (SpO2) is lower in type 1 diabetes (T1D) compared with nondiabetic controls. Hypoxia (low tissue oxygenation) is thought to be a risk factor for progression of diabetic complications, but it is unknown whether hypoxemia (low SpO2) is associated with diabetic complications.

Objective
To test if hypoxemia is associated with presence of diabetic complications in T1D.

Design, Setting, and Methods
Cross-sectional study in persons with T1D divided by a previously suggested threshold in low (&lt;96%) and high (≥96%) SpO2, measured in the supine position with pulse oximetry. Complications included albuminuria (2 of 3 consecutive measurements ≥30 mg/g), any diabetic retinopathy, neuropathy, and history of cardiovascular disease (CVD). Odds ratios were adjusted for age, diabetes duration, sex, smoking, physical activity, body mass index, systolic blood pressure, and blood hemoglobin.

Results
We included 659 persons, 23 (3.5%) with low and 636 (96.5%) with high SpO2. In total, 151 (23%) had albuminuria, 233 (36%) had retinopathy, 231 (35%) had neuropathy, and 72 (11%) had CVD. The adjusted odds ratio (95% CI, P value) for low vs high SpO2 was 3.4 (1.3-8.7, P = 0.01) for albuminuria, 2.8 (1.0-7.5, P = 0.04) for retinopathy, 5.8 (1.8-18.6, P &lt; 0.01) for neuropathy, and nonsignificant for CVD (0.6 [0.2-2.4, P = 0.51]).

Conclusions
SpO2 below 96% was associated with increased presence of albuminuria, retinopathy, and neuropathy in T1D, but not with CVD. Whether hypoxemia could be a target of intervention to prevent progression in microvascular disease in type 1 diabetes should be investigated.
Automated Detection and Surgical Planning for Focal Cortical Dysplasia with Multicenter Validation
Authors: Mo Jiajie , Zhang Jianguo , Hu Wenhan , Sang Lin , Zheng Zhong , Zhou Wenjing , Wang Haixiang , Zhu Junming , Zhang Chao , Wang Xiu , Zhang Kai 
Published: September 2022

BACKGROUND:In patients with surgically amenable focal cortical dysplasia (FCD), subtle neuroimaging representation and the risk of open surgery lead to gaps in surgical treatment and delays in surgery.OBJECTIVE:To construct an integrated platform that can accurately detect FCD and automatically establish trajectory planning for magnetic resonance–guided laser interstitial thermal therapy.METHODS:This multicenter study included retrospective patients to train the automated detection model, prospective patients for model evaluation, and an additional cohort for construction of the automated trajectory planning algorithm. For automated detection, we evaluated the performance and generalization of the conventional neural network in different multicenter cohorts. For automated trajectory planning, feasibility/noninferiority and safety score were calculated to evaluate the clinical value.RESULTS:Of the 260 patients screened for eligibility, 202 were finally included. Eighty-eight patients were selected for conventional neural network training, 88 for generalizability testing, and 26 for the establishment of an automated trajectory planning algorithm. The model trained using preprocessed and multimodal neuroimaging displayed the best performance in diagnosing FCD (figure of merit = 0.827 and accuracy range = 75.0%-91.7% across centers). None of the clinical variables had a significant effect on prediction performance. Moreover, the automated trajectory was feasible and noninferior to the manual trajectory (χ2= 3.540,P= .060) and significantly safer (overall: test statistic = 30.423,P< .001).CONCLUSION:The integrated platform validated based on multicenter, prospective cohorts exhibited advantages of easy implementation, high performance, and generalizability, thereby indicating its potential in the diagnosis and minimally invasive treatment of FCD.
Coupled myovascular expansion directs cardiac growth and regeneration
Authors: DeBenedittis Paige , Karpurapu Anish , Henry Albert , Thomas Michael C. , McCord Timothy J. , Brezitski Kyla , Prasad Anil , Baker Caroline E. , Kobayashi Yoshihiko , Shah Svati H. , Kontos Christopher D. , Tata Purushothama Rao , Lumbers R. Thomas , Karra Ravi 
Published: September 2022

ABSTRACT
Heart regeneration requires multiple cell types to enable cardiomyocyte (CM) proliferation. How these cells interact to create growth niches is unclear. Here, we profile proliferation kinetics of cardiac endothelial cells (CECs) and CMs in the neonatal mouse heart and find that they are spatiotemporally coupled. We show that coupled myovascular expansion during cardiac growth or regeneration is dependent upon VEGF-VEGFR2 signaling, as genetic deletion of Vegfr2 from CECs or inhibition of VEGFA abrogates both CEC and CM proliferation. Repair of cryoinjury displays poor spatial coupling of CEC and CM proliferation. Boosting CEC density after cryoinjury with virus encoding Vegfa enhances regeneration. Using Mendelian randomization, we demonstrate that circulating VEGFA levels are positively linked with human myocardial mass, suggesting that Vegfa can stimulate human cardiac growth. Our work demonstrates the importance of coupled CEC and CM expansion and reveals a myovascular niche that may be therapeutically targeted for heart regeneration.
Assessment of SARS-CoV-2 vaccination status in SARS-COV-2 infected patients admitted in Dr Ruth K.M. Pfau, Civil Hospital Karachi
Authors: Rasheed Tazeen , Ali Faiza Sadaqat , Zuberi Bader Faiyaz , Sadaf Rabiah 
Published: September 2022

Objectives: To determine the frequency of vaccination status in patients with SARS-CoV-2 infection
Methods: This case-control study was conducted at Dr Ruth KM Pfau Civil Hospital Karachi, Pakistan between September 2021 to October 2021. All patients who had positive PCR on nasopharyngeal swab for SARS-CoV-2 infection were included. Information regarding vaccination status and brand of vaccination administered and duration between the last dose of vaccine and positive PCR was noted. The disease status of patients was classified on admission into severe and non-severe disease.
Results: Study included 143 patients, out of which 58 (40.6%) were males and 85 (59.4%) were females. Majority of our patients (78.3%) were unvaccinated. Frequency of Severe SARS-CoV-2 Infection in fully vaccinated patients was less than in unvaccinated patients. The odds of developing severe COVID infection in unvaccinated patients versus vaccinated was 8.55 times higher (OR = 6.23, 95% CI 2.58-28.35). Proportion of vaccinated females was less as compared to males. Significant differences were found in severity between hypertension (p<.001), diabetes (<.001) and age (p<.001).
Conclusion: The frequency of SARS-CoV-2 infection was greater in unvaccinated patients. The odds of developing severe COVID infection in unvaccinated patients versus vaccinated was 8.55 times higher.
doi: https://doi.org/10.12669/pjms.38.8.5733
How to cite this:Rasheed T, Ali FS, Zuberi BF, Sadaf R. Assessment of SARS-CoV-2 vaccination status in SARS-COV-2 infected patients admitted in Dr Ruth K.M. Pfau, Civil Hospital Karachi. Pak J Med Sci. 2022;38(8):---------. doi: https://doi.org/10.12669/pjms.38.8.5733
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Mental health following an initial period of COVID-19 restrictions: findings from a cross-sectional survey in the Republic of Ireland
Authors: Troya Maria Isabela , Joyce Mary , Khashan Ali , Buckley Claire , Chakraborti Kasturi , Hoevel Philipp , Humphries Rory , Kearney Patricia M. , Kiely Elizabeth , Murphy Mike , Perry Ivan , Arensman Ella 
Published: September 2022

Background: We assessed the mental health of individuals in the general population, during an initial period of easing of COVID-19 restrictions in the Republic of Ireland (RoI). Methods: Data were collected through a nationally representative cross-sectional telephone survey, during the first period of easing of restrictions during the COVID-19 pandemic between May and July 2020. Mental health was examined using the Patient Health Questionnaire Anxiety Depression Scale. Poisson regression analyses were conducted to estimate risk ratios with robust variance estimation of the association between selected demographic factors and the risk of having depression and anxiety symptoms. Results: Of the 1,983 participants, 27.7% (n = 549; 95% CI: 0.26 - 0.30) reported depression and anxiety symptoms, while 74 (3.8%; 95% CI: 0.03 - 0.05) disclosed self-harm and/or suicidal thoughts. Females (RR: 1.60, 95% CI: 1.37 - 1.87, p < 0.0005), employed individuals who experienced a change in work status (RR: 1.50, 95% CI: 1.24 - 1.82, p < 0.0005), participants cocooning due to a health condition (RR: 1.34, 95% CI: 1.08 - 1.66, p< 0.01), participants who were self-isolating (RR: 1.25, 95% CI: 1.03 - 1.51, p=0.025) and moderate-heavy drinkers (RR: 1.27, 95% CI: 1.09 - 1.47, p<0.01) were at increased risk of depression and anxiety. Young people aged 18-29 years and those in the two lowest income categories were most likely to report self-harm and/or suicidal thoughts. Conclusion: As the COVID-19 pandemic continues, with further waves and associated restrictions, the impact on mental health in the population as a whole and in specific subgroups must be considered. Study protocol registration: doi.org/10.12688/hrbopenres.13103.2
Features of non-traumatic spinal cord infarction on MRI: Changes over time
Authors: Kim Bo Ra , Park Kyung Seok , Kim Hyo Jin , Kim Jun Yup , Kim Bo Ram , Lee Eugene , Lee Joon Woo 
Published: September 2022

Background and purpose
Spinal cord infarction (SCI) is difficult to diagnosis using MRI findings. We aimed to suggest the optimal timing of MRI studies for diagnosing SCI.

Materials and methods
This retrospective study was approved by our institutional review board. The requirement for informed consent was waived. MRI scans of SCI patients diagnosed between January 2015 and August 2019 were enrolled in the SCI group and subdivided according to the interval between symptom onset and time of MRI scan (A, within 6 h; B, 6–12 hours; C, 12–24 hours; D, 24–72 hours; E, 3–7 days). Three radiologists analyzed the T2WI scans and evaluated the confidence level of diagnosing SCI using a five-point Likert scale: 1, certainly not; 2, probably not; 3, equivocal; 4, probably yes; 5, certainly yes. Scores of 4 and 5 were defined as “T2WI-positive SCI” and scores of 1–3 were defined as “T2WI-negative SCI”.

Results
The SCI group included 58 MRI scans of 34 patients (mean age, 60.6 ± 14.0 years; 18 women). The T2WI positivity rate was 72.4% (42/58). In contrast to the other subgroups, subgroup A included fewer cases of T2WI-positive SCI (1/4, 25%) than T2WI-negative SCI. A confidence score of 5 was the most common in subgroup D (4/27, 14.8%). Among the 12 patients who underwent MRI studies more than twice, confidence scores increased with time.

Conclusion
In patients with suspected SCI showing equivocal initial MRI findings, follow-up MRI studies are helpful, especially when performed between 24 and 72 hours after symptom onset.