bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological description of Pintomyia (Pifanomyia) veintemillasi n. sp., a new sand fly species from the sub-Andean region of Bolivia
Authors: Martinez Eddy , Leon Renato , Mihalca Andrei Daniel , Dujardin Jean-Pierre , Le Pont François 
Published: September 2022

AbstractBackgroundMost sand fly species are located in the Americas; some act as vectors of leishmaniasis and other human diseases. In Bolivia, about 25% of Neotropical species have been identified, and only a few have been implicated as vectors of cutaneous and visceral leishmaniasis. A new species of anthropophilic sand fly from the sub-Andean region of Alto Beni is described herein.MethodsA large systematic entomological survey was carried out in a subtropical humid forest located in the Marimonos mountain range, at around 900 m altitude, in the municipality of Palos Blancos, Sud Yungas Province, Department of La Paz, Bolivia. Sand flies were captured over a period of 26 months between January 1982 and February 1984, at the ground and canopy level, using both CDC light traps and protected human bait. A total of 24,730 sand flies were collected on the ground, distributed in 16 species, and 3259 in the canopy, with eight species. One of these species was labeled asPintomia(Pifanomyia)nevesi, although certain morphological features allowed us to doubt that it was that taxon. To define the identity of this sand fly, a re-evaluation (this work) was recently carried out through morphological analyses and measurements of the available specimens mounted on Euparal, previously labeled asPi. (Pif.)nevesi.ResultsBased on the morphological traits and measurements, the re-evaluated specimens were definitively identified as a new sand fly species,Pintomyia(Pifanomyia)veintemillasi, closely related toPi. (Pif.)nevesiandPintomyia(Pifanomyia)maranonensiswithin the Evansi series. This new sand fly was the third most numerous anthropophilic species at the floor (6.2%) and the second most numerous anthropophilic at the canopy (35.1%).ConclusionsA new anthropophilic sand fly species is described asPi. (Pif.)veintemillasin. sp. This sand fly species was caught at about 900 m altitude in the Marimonos mountain range, a highly endemic area for cutaneous and mucosal leishmaniasis due toLeishmania(Viannia)braziliensis. Therefore, this species could be involved in the leishmaniasis transmission in the sub-Andean foothills of Alto Beni, Department of La Paz, Bolivia.Graphical Abstract
CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells
Authors: Gong Shixin , Hu Gongcheng , Guo Rong , Zhang Jie , Yang Yiqi , Ji Binrui , Li Gang , Yao Hongjie 
Published: September 2022

AbstractThe CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation and its biological function is still lacking. Here, we show that CTCF can be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (CBP) and deacetylated by histone deacetylase 6 (HDAC6). CTCF-K20 is required for the CTCF interaction with CBP. A CTCF point mutation at lysine 20 had no effect on self-renewal but blocked the mesoderm differentiation of mouse embryonic stem cells (mESCs). The CTCF-K20 mutation reduced CTCF binding to the promoters and enhancers of genes associated with early cardiac mesoderm differentiation, resulting in diminished chromatin accessibility and decreased enhancer-promoter interactions, impairing gene expression. In summary, this study reveals the important roles of CTCF-K20 in regulating CTCF genomic functions and mESC differentiation into mesoderm.
Microbial community regulation and performance enhancement in gas biofilters by interrupting bacterial communication
Authors: Wang Yong-Chao , Lin Yu-Ting , Wang Can , Tong Zhen , Hu Xu-Rui , Lv Ya-Hui , Jiang Guan-Yu , Han Meng-Fei , Deng Ji-Guang , Hsi Hsing-Cheng , Lee Chung-Hak 
Published: September 2022

Abstract
Background
Controlling excess biomass accumulation and clogging is important for maintaining the performance of gas biofilters and reducing energy consumption. Interruption of bacterial communication (quorum quenching) can modulate gene expression and alter biofilm properties. However, whether the problem of excess biomass accumulation in gas biofilters can be addressed by interrupting bacterial communication remains unknown.

Results
In this study, parallel laboratory-scale gas biofilters were operated with Rhodococcus sp. BH4 (QQBF) and without Rhodococcus sp. BH4 (BF) to explore the effects of quorum quenching (QQ) bacteria on biomass accumulation and clogging. QQBF showed lower biomass accumulation (109 kg/m3) and superior operational stability (85–96%) than BF (170 kg/m3; 63–92%) at the end of the operation. Compared to BF, the QQBF biofilm had lower adhesion strength and decreased extracellular polymeric substance production, leading to easier detachment of biomass from filler surface into the leachate. Meanwhile, the relative abundance of quorum sensing (QS)-related species was found to decrease from 67 (BF) to 56% (QQBF). The QS function genes were also found a lower relative abundance in QQBF, compared with BF. Moreover, although both biofilters presented aromatic compounds removal performance, the keystone species in QQBF played an important role in maintaining biofilm stability, while the keystone species in BF exhibited great potential for biofilm formation. Finally, the possible influencing mechanism of Rhodococcus sp. BH4 on biofilm adhesion was demonstrated. Overall, the results of this study achieved excess biomass control while maintaining stable biofiltration performance (without interrupting operation) and greatly promoted the use of QQ technology in bioreactors.

Graphical Abstract
De novo disruptive heterozygous MMP21 variants are potential predisposing genetic risk factors in Chinese Han heterotaxy children
Authors: Qin Xi-ji , Xu Meng-meng , Ye Jia-jun , Niu Yi-wei , Wu Yu-rong , Xu Rang , Li Fen , Fu Qi-hua , Chen Sun , Sun Kun , Xu Yue-juan 
Published: September 2022

Abstract
Background
Heterotaxy syndrome (HTX) is caused by aberrant left–right patterning early in embryonic development, which results in abnormal positioning and morphology of the thoracic and abdominal organs. Currently, genetic testing discerns the underlying genetic cause in less than 20% of sporadic HTX cases, indicating that genetic pathogenesis remains poorly understood. In this study, we aim to garner a deeper understanding of the genetic factors of this disease by documenting the effect of different matrix metalloproteinase 21 (MMP21) variants on disease occurrence and pathogenesis.

Methods
Eighty-one HTX patients with complex congenital heart defects and 89 healthy children were enrolled, and we investigated the pathogenetic variants related to patients with HTX by exome sequencing. Zebrafish splice-blocking Morpholino oligo-mediated transient suppression assays were performed to confirm the potential pathogenicity of missense variants found in these patients with HTX.

Results
Three MMP21 heterozygous non-synonymous variants (c.731G > A (p.G244E), c.829C > T (p.L277F), and c.1459A > G (p.K487E)) were identified in three unrelated Chinese Han patients with HTX and complex congenital heart defects. Sanger sequencing confirmed that all variants were de novo. Cell transfection assay showed that none of the variants affect mRNA and protein expression levels of MMP21. Knockdown expression of mmp21 by splice-blocking Morpholino oligo in zebrafish embryos revealed a heart looping disorder, and mutant human MMP21 mRNA (c.731G > A, c.1459A > G, heterozygous mRNA (wild-type&c.731G > A), as well as heterozygous mRNA (wild-type& c.1459A > G) could not effectively rescue the heart looping defects. A patient with the MMP21 p.G244E variant was identified with other potential HTX-causing missense mutations, whereas the patient with the MMP21 p.K487E variant had no genetic mutations in other causative genes related to HTX.

Conclusion
Our study highlights the role of the disruptive heterozygous MMP21 variant (p.K487E) in the etiology of HTX with complex cardiac malformations and expands the current mutation spectrum of MMP21 in HTX.
Persian version of the work design questionnaire: measurement of its psychometric characteristics
Authors: Kahaki Zeinab Rasouli , Gharibi Vahid , Keshavarz Mojtaba , Cousins Rosanna , Mokarami Hamidreza 
Published: September 2022

AbstractBackgroundWork design questionnaire (WDQ), as a comprehensive and integrative tool, is one of the most important instruments frequently used to assess work characteristics. The aim of this study was to measure the psychometric characteristics of the Persian version of WDQ.MethodsTranslation and cross-cultural adaptation procedures were applied in translating the original WDQ into Persian. A total of 270 participants participated in this study. The validity of the questionnaire were measured using face validity, content validity, convergent validity, and construct validity based on confirmatory factor analysis (CFA). Reliability was assessed through internal consistency.ResultsMean content validity index was 0.95. The CFA results indicated support for a 21-factor solution. There were significant correlations between dimensions of WDQ and both job satisfaction and perceived stress. Cronbach's alpha of all items was 0.87.ConclusionResults indicated that the WDQ exhibited very good psychometric properties and can be applied as a useful tool to assess work characteristics among Iranian employees. Accordingly, the authors recommend its administration in future studies. The work characteristics was significantly associated with job satisfaction and job stress. Therefore, improved work design would reduce negative consequences, such as job stress, and increase positive behaviors, such as job satisfaction.
Estimation of paleo-permeability around a seismogenic fault based on permeability tensor from observable geometric information of quartz veins
Authors: Hosono Hinako , Takemura Takato , Asahina Daisuke , Otsubo Makoto 
Published: September 2022

AbstractThe mineral veins formed by filling tensile cracks record the accumulation of past hydraulic activities such as fluid migration in the damage zones of a fault. The purpose of this study is to estimate the fluid flow behavior around thrust faults using a three-dimensional permeability tensor from the geometric information of mineral veins. Here, the estimated permeability represents paleo-permeability when the mineral veins were open fractures. We attempted to estimate paleo-permeability in the damage zone around the Nobeoka Thrust fault by applying Oda’s permeability tensor theory, as determined from the geometric information of mineral veins observed in the outcrop. In addition, in situ data acquisition and analytic techniques were developed to estimate a three-dimensional paleo-permeability tensor, and we estimated the paleo-permeability around the Nobeoka Thrust. As a result, the paleo-permeability tensor could be estimated from the geometric information of the mineral veins in the outcrop, which helped clarify the migration of fluids around the fault. Our results show that the paleo-permeability anisotropy and paleo-permeability value changed with distance from the fault core on the footwall; in particular, the maximum paleo-permeability increased from the damage zone to the fault core. In addition, the direction of maximum paleo-permeability shows that the fluid in the footwall migrated toward the fault plane or to the hanging wall immediately after the earthquake.
Graphical Abstract
Tools for the efficient analysis of surface waves from active and passive seismic data: exploring an NE-Italy perilagoon area with significant lateral variations
Authors: Dal Moro Giancarlo , Stemberk Josef 
Published: September 2022

AbstractA series of reclamation works that took place during the twentieth century, almost completely destroyed the dune system that characterized the eastern part of the Grado-Marano perilagoonal area (NE Italy). Because of the limited data available, so far very little was known about the local subsurface conditions and the present paper presents the main outcomes of the seismic exploration accomplished with a twofold goal: collecting comprehensive data about the subsurface conditions (which geologists need to be able to reconstruct the formation processes of the local geomorphological elements) and testing a series of efficient and unconventional methodologies based on the analysis of surface waves from both active and passive seismic data. The survey was designed and accomplished also considering the local digital terrain model (DTM) and some resistivity and penetrometer data. In the present paper we focus on three main areas and, from the methodological point of view, special emphasis is given to the Holistic analysis of Surface waves (HS) and the Horizontal-to-Vertical Spectral Ratio (HVSR), since both these techniques require simple field procedures and a light equipment. It is also show the wealth of information that the simple spectral analysis of multi-offset passive data can provide in particular for the identification of possible lateral variations. In fact, in spite of the low-energy depositional environment, the area reveals extremely complex with major and abrupt lateral variations that require special care and prevent from using coarse methodologies that cannot properly handle their identification. Collected geophysical data provide a consistent overall scenario: while the area is in general dominated by soft (silty) sediments, the residual dunes are constituted by cemented sandy materials (medium-grained calcarenite) responsible for anomalously high shear-wave velocity (VS) values already at the surface. Parallel to such residual sandy dunes we also identified a series of peat channels characterized by distinctive low VS values due to a significant amount of organic components.
Graphical Abstract
Mechanical consequences at the tendon-bone interface of different medial row knotless configurations and lateral row tension in a simulated rotator cuff repair
Authors: Maia Dias Carlos , Gonçalves Sérgio B. , Completo António , da Silva Manuel Ribeiro , de Campos Azevedo Clara , Mineiro Jorge , Ferreira Frederico , Folgado João 
Published: September 2022

AbstractPurposeLittle is known about the direct influence of different technical options at the rotator cuff tendon-bone interface (TBI) and, more specifically, at the medial bearing row (MBR), regarding local contact force, area and pressure. We evaluated the mechanical repercussions of different medial row anchor configurations for that setting using different values of tension in the lateral row anchors.MethodsKnotless transosseous equivalent (TOE) rotator cuff repairs with locked versus nonlocked medial anchors and single versus double-hole suture passage were tested in a synthetic rotator cuff mechanical model, using 2 different values of lateral row tension. Contact force, area, pressure, peak force and MBR force were compared at the simulated TBI using a pressure mapping sensor.ResultsWhen compared to locked anchors, medial row sliding configurations generate lower values for all the above-mentioned parameters.The use of double-hole suture passage in the medial cuff generated slightly higher values contact area regardless of lateral row tension. At higher lateral row tension values, lower values of the remaining parameters, including MBR force, were found when compared to single-hole suture passage.Lateral row anchor tension increase induced an increase of all parameters regardless of the medial row configuration and TBI contact force and MBR force were the most susceptible parameters, regardless of the medial row pattern.ConclusionMedial row mechanism, suture configuration and lateral row tension interfere with the mechanical force, area and pressure at by TBI. Lateral row tension increase is a major influencer in those parameters.These results can help surgeons choose the right technique considering its mechanical effect at the TBI.
Aortic repair following initial decompressive craniectomy for acute type A aortic dissection complicated with extensive hemorrhagic cerebral infarction: a case report
Authors: Kano Masaki , Iwahori Akinari , Ogino Hitoshi 
Published: September 2022

AbstractA 69-year-old woman presented with acute type A aortic dissection complicated by extensive hemorrhagic cerebral infarction due to brain malperfusion. Emergency decompressive craniectomy was initially performed, with an initial diagnosis of hemorrhagic cerebral infarction. The patient was referred for surgical management following a diagnosis of acute type A aortic dissection. After stabilizing the neurological condition with medical treatment for nine weeks, hemiarch replacement was performed electively. The postoperative course was uneventful, with no new neurological disorders. Subsequently, she recovered sufficiently to have daily conversations and attend hospital appointments using a wheelchair.
Primary spindle cell tumor originating from the liver that was difficult to diagnose
Authors: Ikehara Tomohiko , Shimizu Tadaaki , Hirano Shohei , Fukushima Kentaro , Yoshizawa Jun-ichi , Nakamura Toshitsugu , Nakayama Ataru 
Published: September 2022

Abstract
Background
It has been reported that hepatocellular carcinoma (HCC) with spindle cell tumor accounts for 1.8% of all HCCs, but spindle cell tumors that do not show an obvious conventional HCC are extremely rare. In this report, we describe a case of resection of a primary spindle cell tumor of the liver that was difficult to diagnose.

Case presentation
A 75-year-old man presented with fever and right chest pain. He was suspected of a giant primary diaphragmatic tumor of extrahepatic origin by imaging studies. The preoperative differential diagnosis included benign masses such as myxoid sarcoma and schwannoma, and we planned a diaphragmatic resection. Intraoperatively, however, dissection of the tumor from the liver was not possible, requiring an extended right posterior segmentectomy with combined resection of the diaphragm. The patient had a good postoperative course and 1 year has passed since the surgery with no recurrence. The pathology showed that the mass was located just below the hepatic capsule/parenchymal region and was adherent to the diaphragm, but there was no continuity. The morphology suggested a low-grade mesenchymal tumor such as a solitary fibrous tumor and perivascular epithelioid cell tumor, but immunostaining was negative, making the diagnosis difficult. Although some areas of high proliferative activity were observed, finally, the diagnosis of primary spindle cell tumor of the liver with smooth muscle differentiation was made based on the positive results of muscle markers such as αSMA, desmin, and h-caldesmon.

Conclusions
Spindle cell tumor arising from the liver is so rare that preoperative and pathological diagnosis is often difficult to reach. Although further studies are needed to elucidate and better understand this uncommon clinical entity, we consider that complete resection is necessary for the above case, which may contribute to long-term survival.
miR-31-5p from placental and peripheral blood exosomes is a potential biomarker to diagnose preeclampsia
Authors: Zou Gang , Ji Qingfang , Geng Zixiang , Du Xiling , Jiang Lingyan , Liu Te 
Published: September 2022

Abstract
Background
Preeclampsia, a multisystem disorder of unknown etiology, is one of the leading causes of maternal and perinatal morbidity and mortality. Identifying sensitive, noninvasive markers can aid its prevention and improve prognosis. microRNAs (miRs), which function as negative regulators of gene expression, are closely related to preeclampsia occurrence and development. Herein we investigated the relationship between the DLK1-Dio3 imprinted miR cluster derived from placental and peripheral blood exosomes of pregnant women with preeclampsia and routine clinical diagnostic indicators, and also determined its potential as a noninvasive diagnostic marker.

Methods
Exosomes were extracted from the placenta and peripheral blood of pregnant women with preeclampsia.

Results
qPCR data indicated that the expression level of miRs, such as miR-134, miR-31-5p, miR-655, miR-412, miR-539, miR-409, and miR-496, in pregnant women with preeclampsia was significantly lower than that in healthy controls; miR-31-5p expression was the most different. Gene ontology analysis predicted that genes negatively regulated by miR-31-5p were mainly enriched in cellular entity, cellular process, and binding; moreover, Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that genes were involved in gonadotropin-releasing hormone receptor pathway and other signaling pathways. Correlation analysis revealed that miR-31-5p was significantly negatively correlated with clinical indicators of preeclampsia, such as systolic and diastolic pressure, lactate dehydrogenase, and proteinuria.

Conclusion
We believe that exosome-derived miR-31-5p can serve as an effective and sensitive biomarker to determine the course of preeclampsia in pregnant women.
Laboratory test of Beauveria bassiana (Balsamo-Crivelli) Vuillemin s.l. (Hypocreales: Clavicipitaceae) baits for the biocontrol of the Toad grasshopper pest, Bufonacris claraziana (Saussure) (Orthoptera: Tristiridae)
Authors: Mariottini Yanina , Lange Carlos E. , Pelizza Sebastian E. 
Published: September 2022

Abstract
Background
The Toad grasshopper pest, Bufonacris claraziana (Saussure) (Orthoptera: Tristiridae) is an endemic species of the Argentine Patagonia region. Among the Tristiridae family, it is the only species recognized as harmful to agricultural and livestock activities in the country. Outbreaks of B. claraziana have become a recurring phenomenon in the recent years, affecting different areas of the Patagonian provinces. The aim of this study was to evaluate, as laboratory bioassays, the mortality in young nymphs of this species treated with bait formulations of the entomopathogenic fungus Beauveria bassiana (Hypocreales: Clavicipitaceae).

Results
The two treatments performed, one of them (I) with conidia of B. bassiana only and the other (II) with conidia of B. bassiana plus canola oil as phagostimulant, produced significantly higher mortality than the control (p < 0.05). At 10 days from the start of the bioassay, the mortality registered in the treatment II (53.33%) was higher than that in the treatment I (23.33%) (p < 0.05). This difference was similar at 15 days post-treatment, mortality reached in treatment II (93.33%) higher than in treatment I (73.33%).

Conclusions
Results demonstrated that the combination of canola oil with wheat bran makes the bait with conidia of B. bassiana more attractive for nymphs of B. claraziana, enhancing mortality over a shorter interval of time.
Preoperative flexible bronchoscopy-aided cannulation of a neonatal H-type tracheo-oesophageal fistula assists intraoperative identification of the fistulous tract
Authors: Procopiuc Livia , Naqvi Shehryer , Yardley Iain , Nyman Andrew G. 
Published: September 2022

Abstract
Background
The surgical repair of H-type tracheo-oesophageal fistulas situated below the level of the second thoracic vertebra requires an open thoracotomy or a thoracoscopy. We describe a novel technique that allows for the use of a cervical incision to repair a fistula situated in the thorax, thus diminishing surgical risk.

Case presentation
In this report, we describe a 3-day-old term baby with an H-type tracheo-oesophageal fistula where flexible bronchoscopy and gastroscopy were used to cannulate the fistula with a soft ureteric catheter. This allowed for it to be tractioned into the cervical region where it was surgically dissected and isolated. There was no need for re-intervention in the first 3 months after surgery.

Conclusion
Flexible bronchoscopy-aided cannulation of H-type fistulas can assist in intraoperative identification of the fistulous tract as well as help traction it into a surgically more accessible area like the cervical region.