bluuebunny/crossref_metadata_2025_split huggingface dataset:
Edukasi Nutrisi dalam Pencegahan Stunting dan Implementasi Senam Hamil di Puskemas Sidomulyo Rawat Inap Pekanbaru
Authors: Agritubella Syafrisar Meri , Jannah Fathul 
Published: September 2022

Nutrition education and the implementation of pregnancy exercises help mothers improve maternal health, prevent anemia and babies born with low weight. Maintaining the consumption pattern of pregnant women also prevents children from experiencing stunting. The purpose of this community service was to provide education and implementation of pregnancy exercise to improve the health status of pregnant women at the Sidomulyo Public Health Center, RI. The method used is the provision of health education and assessing the mother's understanding of nutrition and assessing the implementation of pregnancy exercise for pregnant women. The activity was carried out 3 times, starting with a pre-test and post-test related to knowledge of pregnant women and observing the implementation of pregnancy exercise. The number of pregnant women who participated in this activity was 9 people. The results showed that there was an increase in the average value of knowledge of pregnant women by 24.29. The results of the implementation of pregnancy exercise showed that 100% of pregnant women carried out pregnancy exercise in the good category. It is hoped that there will be an increase in the health status of pregnant women therefore that they do not experience anemia and normal birth weight babies and growth and development according to their age.

ABSTRAK
Edukasi nutrisi dan pelaksanaan senam hamil membantu ibu meningkatkan derajat kesehatan, mencegah anemia dan bayi lahir dengan berat badan rendah. Pentingnya menjaga pola konsumsi ibu hamil juga mencegah anak mengalami stunting. Tujuan pengabdian masyarakat ini adalah pemberian edukasi dan pelaksanaan senam hamil untuk meningkatkan derajat kesehatan ibu hamil di Puskesmas Sidomulyo RI. Metode yang digunakan adalah pemberian pendidikan kesehatan dan menilai pemahaman ibu tentang nutrisi dan menilai pelaksanaan senam hamil pada ibu hamil. Pelaksanaan kegiatan sebanyak 3 kali dimulai dengan adanya pre–test dan post-test terkait pengetahuan ibu hamil dan observasi pelaksanaan senam hamil. Jumlah ibu hamil yang mengikuti kegiatan ini sebanyak 9 orang. Hasil didapatkan bahwa terdapat peningkatan nilai rata-rata pengetahuan ibu hamil sebesar 24,29. Hasil pelaksanaan senam hamil didapatkan bahwa 100% ibu hamil melaksanakan senam hamil pada kategori baik. Diharapkan adanya peningkatan derajat kesehatan ibu hamil sehingga tidak mengalami anemia dan berat badan bayi lahir normal serta pertumbuhan dan perkembangan sesuai dengan usianya.
Angle-stable interlocking nailing in a canine critical-sized femoral defect model for bone regeneration studies: In pursuit of the principle of the 3R’s
Authors: Saunders W. B. , Dejardin L. M. , Soltys-Niemann E. V. , Kaulfus C. N. , Eichelberger B. M. , Dobson L. K. , Weeks B. R. , Kerwin S. C. , Gregory C. A. 
Published: September 2022

Introduction: Critical-sized long bone defects represent a major therapeutic challenge and current treatment strategies are not without complication. Tissue engineering holds much promise for these debilitating injuries; however, these strategies often fail to successfully translate from rodent studies to the clinical setting. The dog represents a strong model for translational orthopedic studies, however such studies should be optimized in pursuit of the Principle of the 3R’s of animal research (replace, reduce, refine). The objective of this study was to refine a canine critical-sized femoral defect model using an angle-stable interlocking nail (AS-ILN) and reduce total animal numbers by performing imaging, biomechanics, and histology on the same cohort of dogs.Methods: Six skeletally mature hounds underwent a 4 cm mid-diaphyseal femoral ostectomy followed by stabilization with an AS-ILN. Dogs were assigned to autograft (n = 3) or negative control (n = 3) treatment groups. At 6, 12, and 18 weeks, healing was quantified by ordinal radiographic scoring and quantified CT. After euthanasia, femurs from the autograft group were mechanically evaluated using an established torsional loading protocol. Femurs were subsequently assessed histologically.Results: Surgery was performed without complication and the AS-ILN provided appropriate fixation for the duration of the study. Dogs assigned to the autograft group achieved radiographic union by 12 weeks, whereas the negative control group experienced non-union. At 18 weeks, median bone and soft tissue callus volume were 9,001 mm3 (range: 4,939–10,061) for the autograft group and 3,469 mm3 (range: 3,085–3,854) for the negative control group. Median torsional stiffness for the operated, autograft treatment group was 0.19 Nm/° (range: 0.19–1.67) and torque at failure was 12.0 Nm (range: 1.7–14.0). Histologically, callus formation and associated endochondral ossification were identified in the autograft treatment group, whereas fibrovascular tissue occupied the critical-sized defect in negative controls.Conclusion: In a canine critical-sized defect model, the AS-ILN and described outcome measures allowed refinement and reduction consistent with the Principle of the 3R’s of ethical animal research. This model is well-suited for future canine translational bone tissue engineering studies.
A new mouse model of ankle instability induced by multiple mechanical sprains with controlled inversion angle and speed
Authors: Ching Shih-Hong , Chiu Yen-Chun , Liao Yu-Ching , Yang Shang-Hsun , Tsai Yi-Ju 
Published: September 2022

Ankle sprain occurs by a sudden and extreme inversion and plantarflexion at the ankle joint to cause ligamentous injuries. A portion of ankle sprain patients experience recurrent ankle sprains and develop chronic ankle instability (CAI). The present CAI animal models are single events with severe ligamentous injury using surgical transection of ligaments or manually overextending the ankle.Purpose: To simulate the mechanical and recurrent sprain injuries in CAI patients, we established a new ankle instability model with multiple ankle injuries using a self-designed machine to sprain the ankle with a controlled inversion angle and speed.Methods: Male C57BL/6J mice were used and respectively subjected to a sham operation, calcaneofibular ligament (CFL) transection, and mechanical ankle sprains. Three mechanical sprains were performed on the 13th and 185th day after the initial mechanical ankle sprain.Results: The first mechanical sprain and CFL transection induced ankle injury as indicated by an average of a 62% decrease in ankle pressure pain threshold and a 114% increase in the ankle thickness compared with the contralateral untreated ankle. The second and third mechanical sprains induced recurrent ankle injuries. The foot slips during beam tests were increased after mechanical ankle sprains but not after CFL transection, indicating the induction of motor balance deficits. Multiple mechanical ankle sprains induced significant gait changes in longer duration of stance (an average of 194% increase), swing (134%), and step cycle (147%) compared with CFL transection or sham operation, and slower walking speed (78% reduction) and shorter step distance (91%) after the third sprain.Conclusion: These results elucidate that multiple mechanical sprains, which induce recurrent ankle injuries, balance deficits, and gait changes, are a good model for investigating the mechanisms of CAI induced by recurrent sprain injuries.
Health risk assessment of heavy metals in soils and food crops from a coexist area of heavily industrialized and intensively cropping in the Chengdu Plain, Sichuan, China
Authors: Liu Qing , Li Xiaohui , He Lei 
Published: September 2022

Environmental pollution caused by rapid industrial activities are becoming increasingly drastic, particularly its impact on soil and plant health. The present study was conducted to investigate the heavy metal (loid) (As, Cd, Cu, Hg, Pb, and Zn) concentrations in soils and food biomass crops and estimate the potential health risks of metals to humans via consumption of contaminated food biomass crops from Shifang, a periurban agricultural areas in the Chengdu Plain, Sichuan, China. Results revealed that the soils have been experiencing a substantial accumulation of heavy metals, especially for Cd, with a mean of 0.84 mg kg−1, about six times higher than the background values, of which 98% exceeded the pollution warning threshold of the China Soil Environmental Quality Standards. A total of 78% of all the grain part failed the national food standard for Cd. No significantly positive relationships between metal levels in food biomass crops and in the corresponding soils, indicated metals enrichment in soils were not entirely reflected to crops contaminant burdens. Estimated daily intake (EDI) of all the metals except for Pb, exceeded the oral reference dose (RfD) or the minimal risk levels recommended by USEPA and ATSDR. Target hazard quotients (THQs) of all the metals except for Cd was less than one indicated that potential health risk to the local inhabitant originated mainly from Cd exposure via cereals consumption. Mitigation strategies to curtail Cd-contaminated soils and crops Cd burdens need careful tailoring to meet the needs of health and safety in this region.
Evaluation of environmental Mucorales contamination in and around the residence of COVID-19-associated mucormycosis patients
Authors: Ghosh Anup K. , Singh Ravinder , Reddy Snigdha , Singh Shreya , Rudramurthy Shivaprakash M. , Kaur Harsimran , Choudhary Hansraj , Chakrabarti Arunaloke 
Published: September 2022

IntroductionRecently, India witnessed an unprecedented surge of coronavirus disease 2019 (COVID-19)-associated mucormycosis (CAM) cases. In addition to patient management issues, environmental Mucorales contamination possibly contributed to the outbreak. A recent study evaluated environment contamination by Mucorales in the hospital setting. However, a considerable number of CAM patients were never admitted to a hospital before the development of the disease. The present study, therefore, planned to evaluate Mucorales contamination of patients’ residences.MethodsThe residential environment of 25 patients with CAM living in north India was surveyed. Air samples were collected from indoor and immediate outdoor vicinity of the patients’ residence and cultured on Dichloran Rose–Bengal Chloramphenicol (DRBC) agar with benomyl for selective isolation of Mucorales. Surface swab samples were also collected from the air coolers fitted in those residences and cultured on DRBC agar. The isolates were identified by phenotypic and genotypic methods. Amplified fragment length polymorphism (AFLP) was employed to evaluate the genetic relatedness of the environmental and patients’ clinical isolates.ResultsThe median spore count (mean ± SD, cfu/m3) of Mucorales in the air of patients’ bedrooms was significantly higher than in the air in other rooms in those residences (3.55 versus 1.5, p = 0.003) or the air collected directly from the front of the air cooler (p &lt; 0.0001). The Mucorales spore count in the environment did not correlate with either ventilation of the room or hygiene level of the patients’ residences. Rhizopus arrhizus was isolated from the environment of all patients’ residences (n = 25); other Mucorales species isolated were Cunninghamella bertholletiae (n = 14), Rhizopus microsporus (n = 6), Rhizopus delemar (n = 6), Syncephalastrum racemosum (n = 1), Lichtheimia corymbifera (n = 1), and Mucor racemosus (n = 1). Genetic relatedness was observed between 11 environmental isolates from the patients’ bedrooms and respective clinical isolates from patients.DiscussionThe study supported the view that the patients might have acquired Mucorales from the home environment during the post-COVID-19 convalescence period. Universal masking at home during patients’ convalescence period and environmental decontamination could minimize exposure in those susceptible patients.
Inflammatory bowel disease and cardiovascular disease: A two-sample Mendelian randomization analysis
Authors: Wu Kaiwen , Li Aoshuang , Liu Lei , Shu Tao , Xia Demeng , Sun Xiaobin 
Published: September 2022

BackgroundAlthough epidemiological studies have shown a positive relationship between inflammatory bowel disease (IBD) and risk of cardiovascular disease (CVD) outcomes, a solid causal relationship has not been established. Thus, a two-sample Mendelian randomization (MR) study was conducted to explore the potential causal effect between IBD and CVD outcomes.MethodsWe performed a two-sample MR analysis to analyze the causal effect of the IBD on CVD outcome by using summary-level genome-wide association studies of European descent. The inverse-variance weighted (IVW) method was used as the main MR analysis, with complementary analyses of MR Egger, maximum likelihood, weighted median, penalized weighted media, simple mode, weighted mode, and MR-PRESSO methods. Multiple sensitivity analyses were used to evaluate the robustness of our results.ResultsAll P-values were greater than 0.05 in the IVW method, showing no evidence of a causal association between circulating IBD and CVD. Similar results were observed by using other MR methods. No evidence of heterogeneity, pleiotropy, or outlier single-nucleotide polymorphisms was detected. Sensitivity analyses demonstrated the robustness of the results.ConclusionThe findings of this study provided no evidence to support that IBD has a large effect on risk of CVD outcomes, which is in contrast to many previous observational reports. Further studies are needed to determine the potential mechanism of association identified in observational studies.
A newly designed disk-lobe occluder with isogenous barbs for left atrial appendage closure: Initial multicenter experience
Authors: Bai Yuan , Tang Xuechao , Xu Xudong , Zhao Xianxian , Xu Yawei , Chen Wei , Zhu Xianyang , Wang Qiguang , Han Zhihua , Wang Changqian , He Lu , Zhang Yushun , Pan Xin , Wang Cheng , Chen Lianglong , Cen Xuejiang , Qu Baiming , Zhu Ni , Zhang Sha , Huang Xinmiao , Qin Yongwen 
Published: September 2022

BackgroundAlthough the implant success rate of left atrial appendage closure (LAAC) has increased and complications have decreased over time, there are still anatomically and technically complicated cases where novel LAA occluders may simplify the procedure and thus might potentially improve the clinical outcome.ObjectivesThis study aimed to assess the safety and efficacy of the newly designed device with isogenous barbs in LAAC.MethodsEight centers in China participated in this prospective study from July 2016 to April 2018. Peri- and post-procedural safety and efficacy were evaluated through scheduled follow-ups and transesophageal echocardiography (TEE).ResultsA total of 175 patients with a mean age of 68.4 ± 9.2 years old, a mean CHA2DS2-VASc score of 4.7 ± 1.8, and a mean HAS-BLED score of 3.2 ± 1.3, were included. The device was successfully implanted in 173 patients (98.9%). The device size ranged from 18 to 34 mm. Clinically relevant pericardial effusion (PEF) in the perioperative period, occurred in 3 patients (1.7%). TEE follow-up was available in 167 (96.5%) patients at 12-month. During follow-up, 9 patients suffered serious adverse event: 4 death (2.3%), 1 ischemic stroke (0.6%), and 2 gastro-intestinal bleeding (1.2%) and 2 device-related thrombus (DRT) (1.2%). Estimated annual thromboembolism rate reduced by 90% and estimated annual major bleeding rate reduced by 81% after LAAC with the newly designed device.ConclusionThe newly designed device with isogenous barbs for LAAC could be performed effectively with a low incidence of adverse events and a high incidence of anatomic closure.
Sleep duration, daytime napping, and risk of incident stroke: Nuances by metabolic syndrome from the China health and retirement longitudinal study
Authors: Fang Yuanyuan , He Yuqin , Huang Yanzhu , Ran Lusen , Song Wenhui , Hao Jiahuan , Yao Di , Li Rong , Pan Dengji , Qin Tingting , Wang Minghuan 
Published: September 2022

Background and purposeThe relationship between sleep duration and stroke are inconclusive in China, especially in those individuals with metabolic syndrome. We aimed to investigate the association between sleep duration and incident stroke in participants with metabolic syndrome or its specific components in China.Materials and methodsData were taken from the 2011 and 2015 waves of China Health and Retirement Longitudinal Study (CHARLS). Habitual sleep duration (≤6, 6∼8 [reference], &gt;8 h), daytime napping (0, 1∼60 [reference], and &gt;60 min) were determined by self-reported questionnaires. Metabolic syndrome was defined by blood assessment and biomarkers combined with self-reported doctors’ diagnosis. Incident stroke was determined by reported stroke from 2011 to 2015 wave. Cross-sectional and longitudinal associations between sleep and (incident) stroke at baseline and 4-year follow-up period were tested among the population with metabolic syndrome and its components.ResultsA U-shaped relationship was observed between sleep duration and stroke in cross-sectional analysis. Sleep ≤ 6 h/night had a greater risk of incident stroke (hazard ratio [HR] 1.65; 95% confidence interval [CI] 1.04–2.61) compared with sleep 6∼8 h/night. And the HR of stroke was 1.62 (95%CI, 1.03–2.53) for sleep &lt; 7 h/day compared to 7∼9 h/day. These associations were more evident in the female and individuals aged 45–65 years. Furthermore, the effect of short sleep duration on incident stroke was different in each component of metabolic syndrome, which was more pronounced in participants with elevated blood pressure. And a significant joint effect of sleeping ≤ 6 h/night and no napping on risk of stroke was observed (HR 1.82, 95%CI 1.06–3.12).ConclusionShort sleep duration was an independent risk factor for incident stroke, especially among females, individuals aged 45–65 years, or those with some components of metabolic syndrome, such as hypertension. Napping could buffer the risk of short sleep duration on incident stroke.
Stratigraphy and eruption history of maars in the Clear Lake Volcanic Field, California
Authors: Ball Jessica L. 
Published: September 2022

The Clear Lake Volcanic Field (CLVF) is the northernmost and youngest field in a chain of volcanic fields in the California Coast Range mountains. Effusive and explosive volcanic activity in the field has spanned at least 2.1 million years, with the youngest eruptions comprising a series of maar craters at the edges of, and within, Clear Lake itself. This work documents the first direct ages for many of these maar deposits and builds the stratigraphic basis for interpreting eruptive processes and dynamics of the young eruptions which produced them. Detailed stratigraphy has distinguished maar eruption products from pyroclastic deposits (monolithologic falls and flows, previously mapped together with maars as a single unit) and established a set of six eruption facies from maar deposit lithology, grain size parameters, and depositional structures. Radiocarbon dates from carbon films found on clasts at three outcrops have constrained several of these maar eruptions to ∼8,500–13,500 years BP, coinciding with eruptive periods previously estimated based on lake core tephrachronology. Part of this period also coincides with indigenous inhabitation (&lt;12,000 years BP), which suggests that oral histories of Pomo and other local tribes may contain descriptions of volcanic phenomena experienced by local residents of the CLVF. Collaboration between volcanologists and indigenous historians may add a valuable human dimension to the youngest eruptions of the Clear Lake Volcanic Field, and help inform future volcanic hazard assessment.
Co-application of organic amendments and inorganic P increase maize growth and soil carbon, phosphorus availability in calcareous soil
Authors: Khan Khuram Shehzad , Ali Muhammad Moaaz , Naveed Muhammad , Rehmani Muhammad Ishaq Asif , Shafique Muhammad Waleed , Ali Hayssam M. , Abdelsalam Nader R. , Ghareeb Rehab Y. , Feng Gu 
Published: September 2022

Phosphorus (P) constraint can be alleviated by increasing C inputs, which can help to improve crop production and P fertilizer use efficiency. However, the effects of different manures on soil microbial biomass P (MBP) and P fractions as well as C fractions in calcareous soils remain poorly understood. Soil MBP pool involves the P mineralization and immobilization processes, potentially changing P fractions and P availability. Therefore, the effects of different manures on soil microbial biomass (MBP, MBC) pool, P, and C fractions and crop P utilization were evaluated in greenhouse experiments with maize plantation. Treatments included no manure (control), poultry manure (PM), cow manure (CM), goat manure (GM), mixed manure (MM), and three inorganic P (Pi) rates; P0: 0 mg kg-1, P50: 50 mg kg-1, and P100: P100 mg kg-1 (P2O5). For plant growth comparison, crop physiological growth indices, shoot P contents and total P uptake were increased by PM and P100 as compared to other treatments. The PM with P100 significantly increased the plant growth by inducing P uptake of ∼18% compared with control. The results exhibited that Pi (P100) combined with manure (PM) significantly (p &lt; 0.05) increased the soil physicochemical properties, that is, 683.76 mg kg-1 total P, 21.5 mg kg-1 Olsen P, 4.26 g kg-1 SOC, 2.41 g kg-1 POC, as well as microbial biomass C and P increased by 152.84 mg kg-1 and 36.83 mg kg-1, respectively. Consequently, we concluded that PM with Pi (P100) application builds up soil microbial biomass, which is more beneficial for promoting P utilization for maize.
Association of genetic variants in ULK4 with the age of first onset of type B aortic dissection
Authors: Huang Lihong , Tang Jiaqi , Lin Lijuan , Wang Ruihan , Chen Feng , Wei Yongyue , Si Yi , Fu Weiguo 
Published: September 2022

Background: The association between autophagy, structural alterations of the aortic wall, and endothelial dysfunction in humans has yet to be fully elucidated. The family of ULK (UNC51-like) enzymes plays critical roles in autophagy and development. This study aimed to evaluate the association between ULK gene family members and patient age of first type B aortic dissection (TBAD) onset.Methods: The genotype data in a TBAD cohort from China and the related summary-level datasets were analyzed. We applied the sequence kernel association test (SKAT) to test the association between single-nucleotide polymorphisms (SNPs) and age of first onset of TBAD controlling for gender, hypertension, and renal function. Next, we performed a 2-sample Mendelian randomization (MR) to explore the potential causal relationship between ULK4 and early onset of TBAD at the level of gene expression coupled with DNA methylation with genetic variants as instrumental variables.Results: A total of 159 TBAD patients with 1,180,097 SNPs were included. Concerning the association between the ULK gene family and the age of first onset of the TBAD, only ULK4 was found to be significant according to SKAT analysis (q-FDR = 0.0088). From 2-sample MR, the high level of ULK4 gene expression was related to a later age of first onset of TBAD (β = 4.58, p = 0.0214).Conclusion: This is the first study of the ULK gene family in TBAD, regarding the association with the first onset age. We demonstrated that the ULK4 gene is associated with the time of onset of TBAD based on both the SKAT and 2-sample MR analyses.