bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of COVID-19 on disease epidemiology, family dynamics, and social justice in Minnesota: All that you cannot see
Authors: Schleiss Mark R. , Blazar Bruce , Chapman Emily P. , Cutler Gretchen J. , Cutts Diana B. , Eder Milton Mickey , Li Shengxu , Mason Susan M. , Bretscher Brianna M. , Neglia Joseph P. , Scal Peter B. , Winter Stuart S. 
Published: June 2022

Abstract

Objective:
The COVID-19 pandemic presented a challenge to established seed grant funding mechanisms aimed at fostering collaboration in child health research between investigators at the University of Minnesota (UMN) and Children’s Hospitals and Clinics of Minnesota (Children’s MN). We created a “rapid response,” small grant program to catalyze collaborations in child health COVID-19 research. In this paper, we describe the projects funded by this mechanism and metrics of their success.

Methods:
Using seed funds from the UMN Clinical and Translational Science Institute, the UMN Medical School Department of Pediatrics, and the Children’s Minnesota Research Institute, a rapid response request for applications (RFAs) was issued based on the stipulations that the proposal had to: 1) consist of a clear, synergistic partnership between co-PIs from the academic and community settings; and 2) that the proposal addressed an area of knowledge deficit relevant to child health engendered by the COVID-19 pandemic.

Results:
Grant applications submitted in response to this RFA segregated into three categories: family fragility and disruption exacerbated by COVID-19; knowledge gaps about COVID-19 disease in children; and optimizing pediatric care in the setting of COVID-19 pandemic restrictions. A series of virtual workshops presented research results to the pediatric community. Several manuscripts and extramural funding awards underscored the success of the program.

Conclusions:
A “rapid response” seed funding mechanism enabled nascent academic-community research partnerships during the COVID-19 pandemic. In the context of the rapidly evolving landscape of COVID-19, flexible seed grant programs can be useful in addressing unmet needs in pediatric health.
The Effect of an Earthquake Experienced During Pregnancy on Maternal Health and Birth Outcomes
Authors: Amarpoor Mesrkanlou Hossein , Ghaemmaghami Hezaveh Seyed Jamal , Tahmasebi Sanaz , Nikniaz Zeinab , Nikniaz Leila 
Published: June 2022

Abstract

Objective:
Maternal health in pregnancy and birth outcomes were compared between pre- and post-Varzaghan earthquake.

Methods:
In this retrospective descriptive study, before and after the earthquake, 550 and 450 women were enrolled respectively. Neonatal weight, height, and head circumference, as well as maternal weight gain and hemoglobin (Hb) levels were obtained using medical records at health centers. Chi-square test and Independent t-test were used to analyze differences in pregnancy outcomes. A P-value less than 0.05 was considered significant.

Results:
A significant increase in inadequate gestational weight gain (44.1% vs 58.9%) was observed (P = 0.043) before and after the earthquake. The mean hemoglobin level in the first trimester before the earthquake was significantly higher than after the earthquake (P = 0.001). Before–after earthquake comparisons showed that the mean birth weight, birth height, and birth head circumference were decreased significantly (P < 0.05). In addition, the rates of preterm birth (18.91% vs 10.90%), abortion (17.11% vs 10.54%), and stillbirth (3.78% vs 1.82%) were increased significantly after the earthquake (P < 0.05).

Conclusions:
Earthquake causes inadequate gestational weight gain and decreased hemoglobin levels, which lead to adverse birth outcomes. More longitudinal and well-designed studies are desired to investigate the longitudinal consequences of disasters on susceptible groups.
Foreign to whom? Constraining the moral foreign language effect on bilinguals’ language experience
Authors: Del Maschio Nicola , Del Mauro Gianpaolo , Bellini Camilla , Abutalebi Jubin , Sulpizio Simone 
Published: June 2022

AbstractThe moral foreign language effect (MFLE) describes how people’s decisions may change when a moral dilemma is presented in either their native (NL) or foreign language (FL). Growing attention is being directed to unpacking what aspects of bilingualism may influence the MFLE, though with mixed or inconclusive results. The current study aims to bridge this gap by adopting a conceptualization of bilingualism that frames this construct as a composite and continuous measure. In a between-group analysis, we asked 196 Italian–English bilinguals to perform a moral dilemmas task in either their NL (i.e., Italian) or FL (i.e., English). In a within-group analysis, we evaluated the effects of FL age of acquisition, FL proficiency, and language dominance – all measured as continuous variables – on moral decision-making. Overall, findings indicate that differences within bilinguals’ language experience impact moral decisions in an FL. However, the effect of the linguistic factors considered was not ubiquitous across dilemmas, and not always emerged into a MFLE. In light of these results, our study addresses the importance of treating bilingualism as multidimensional, rather than a unitary variable. It also discusses the need to reconceptualize the FLE and its implications on moral decision-making.
The natural occurring RRR-α-Tocopherol and synthetic α-Tocopherol stereoisomers in maternal plasma, cord plasma and breast milk among six regions of China
Authors: Lou Zeru , Dang Guodong , Wu Shiyin , Wu Ke , Cai Meiqin 
Published: June 2022

AbstractThe study aimed to investigate RRR-α-Tocopherol and synthetic α-Tocopherol stereoisomers in maternal plasma, cord plasma and breast milk from different regions of China, providing a reference for further guidance on maternal diet and the potential need to supplement mothers with vitamin E. Two hundred and twenty-one sample sets from maternal plasma, cord plasma and three stages of milk (colostrum: 1–5 d after delivery; transitional milk: 10–15 d; mature milk: 40–45 d) were collected longitudinally in six regions of Shanghai, Guangzhou, Tianjin, Chengdu, Lanzhou and Changchun. α-Tocopherol and the stereoisomers were determined by HPLC with a fluorescence detector. The RRR configuration accounted for more than 80 % of α-Tocopherol in maternal plasma, cord plasma and breast milk. Overall, there were regional differences both in α-Tocopherol and RRR. There were significant correlations between α-Tocopherol and RRR in maternal plasma, cord plasma and milk (P < 0.001). As well as negative correlations among α-Tocopherol, RRR and weight-related indicators, which refer to pre-gestation weight and BMI, pre-delivery weight and BMI and pregnancy weight gain, in almost all of samples. This study suggested that RRR-α-Tocopherol was the dominant configuration of α-Tocopherol and the main active form of vitamin E in the early life, guiding the rational supplement of pregnant women and the addition of α-Tocopherol in infant formula milk powder.
Parents tune their vowels to the emergence of children’s words
Authors: ODIJK Lotte , GILLIS Steven 
Published: June 2022

AbstractThe aim of this study was to investigate the acoustic vowel space area in infant directed speech (IDS). The research question is whether the vowel space is expanded or remains constant in IDS. A corpus of spontaneous interactions of 9 dyads followed monthly from the age of 6 to 24 months was analyzed. The occurrences in the parents’ speech of each word that the children eventually acquired were extracted. The surface of the vowel triangle and the convex hull of all vowels were computed. The main result is that the development of the vowel space in IDS follows an inverted U-shaped curve: the vowel space starts relatively small, gradually increases as the child’s first word use approaches, and decreases again afterwards. These findings show that parents adapt their articulation to the evolving linguistic abilities of their child, and this adaptation can be detected at the level of individual lexical items.
A Storm, An Explosion, and Flying Rocks - An Unusual Injury due to a Lightning Strike in the Mountains
Authors: Kosiński Sylweriusz , Aleksandrowicz Dawid , Serafinowicz Zofia 
Published: June 2022

AbstractBackground:Most injuries observed in victims of lightning strikes can be explained by electrothermal phenomena. Blast penetrating injuries caused by a lightning-strike-induced explosion of a nearby structure are rarely reported.Case Presentation:Here reported is the case of a patient with numerous mixed injuries caused by a lightning strike, including deep lacerations of both hips and thighs with rock fragments embedded in the wounds. Surgical removal of rock fragments from deep areas of the right hip and right lower leg was necessary. The cause of the formation of rock missiles was the lightning-strike-induced explosion of rock. Rapid evaporation of water enclosed in rock crevices was presumably the main force underlying the explosion.Conclusion:Blast penetrating injuries should be considered and excluded in all patients struck by lightning, particularly when occurring in rocky terrain. The diagnosis and treatment of such injuries can be difficult and require special preparation.
Is spiritual well-being related to survival time of inpatients with advanced cancer? An East Asian cohort study
Authors: Hiratsuka Yusuke , Suh Sang-Yeon , Won Seon Hye , Choi Sung Eun , Kim Sun-Hyun , Cheng Shao-Yi , Chen Ping-Jen , Yoon Seok-Joon , Koh Su-Jin , Park Shin Ae , Seo Ji-Yeon , Hui David , Yamaguchi Takashi , Morita Tatsuya , Tsuneto Satoru , Mori Masanori , Inoue Akira 
Published: June 2022

AbstractObjectivesIt has been suggested that psychosocial factors are related to survival time of inpatients with cancer. However, there are not many studies examining the relationship between spiritual well-being (SWB) and survival time among countries. This study investigated the relationship between SWB and survival time among three East Asian countries.MethodsThis international multicenter cohort study is a secondary analysis involving newly admitted inpatients with advanced cancer in palliative care units in Japan, South Korea, and Taiwan. SWB was measured using the Integrated Palliative Outcome Scale (IPOS) at admission. We performed multivariate analysis using the Cox proportional hazards model to identify independent prognostic factors.ResultsA total of 2,638 patients treated at 37 palliative care units from January 2017 to September 2018 were analyzed. The median survival time was 18.0 days (95% confidence interval [CI] 16.5–19.5) in Japan, 23.0 days (95% CI 19.9–26.1) in Korea, and 15.0 days (95% CI 13.0–17.0) in Taiwan. SWB was a significant factor correlated with survival in Taiwan (hazard ratio [HR] 1.27; 95% CI 1.01–1.59; p = 0.04), while it was insignificant in Japan (HR 1.10; 95% CI 1.00–1.22; p = 0.06), and Korea (HR 1.02; 95% CI 0.77–1.35; p = 0.89).Significance of resultsSWB on admission was associated with survival in patients with advanced cancer in Taiwan but not Japan or Korea. The findings suggest the possibility of a positive relationship between spiritual care and survival time in patients with far advanced cancer.
Building a virtual Roman city: teaching history through video game design
Authors: Forsyth Harrison 
Published: June 2022

AbstractIn October of 2018, a pedagogical experiment was conducted at York University, Toronto, Canada, in which students were given an assignment. For this assignment they were to conduct research on a variety of Roman public buildings in groups, build digital reconstructions of them using the Unity 3D game engine, and present them to the class in the form of a virtual reality (VR) simulation. In doing so, students were able to create a virtual built environment based on their research, navigate it, and discuss the space with a sense of immersion and scale. Using this experiment as a case study, the goal of this article is twofold: firstly, to assess the pedagogical efficacy of constructionist approaches to teaching students about Roman architecture, specifically using VR and video game design technology. The second goal is to address the technical and pedagogical challenges of using game design software in the classroom and to propose ways in which this assignment can be improved in the future.
Detection of Forced Change Within Combined Climate Fields Using Explainable Neural Networks
Authors: Rader Jamin K. , Barnes Elizabeth A. , Ebert‐Uphoff Imme , Anderson Chuck 
Published: June 2022

AbstractAssessing forced climate change requires the extraction of the forced signal from the background of climate noise. Traditionally, tools for extracting forced climate change signals have focused on one atmospheric variable at a time, however, using multiple variables can reduce noise and allow for easier detection of the forced response. Following previous work, we train artificial neural networks to predict the year of single‐ and multi‐variable maps from forced climate model simulations. To perform this task, the neural networks learn patterns that allow them to discriminate between maps from different years—that is, the neural networks learn the patterns of the forced signal amidst the shroud of internal variability and climate model disagreement. When presented with combined input fields (multiple seasons, variables, or both), the neural networks are able to detect the signal of forced change earlier than when given single fields alone by utilizing complex, nonlinear relationships between multiple variables and seasons. We use layer‐wise relevance propagation, a neural network explainability tool, to identify the multivariate patterns learned by the neural networks that serve as reliable indicators of the forced response. These “indicator patterns” vary in time and between climate models, providing a template for investigating inter‐model differences in the time evolution of the forced response. This work demonstrates how neural networks and their explainability tools can be harnessed to identify patterns of the forced signal within combined fields.
Multiple Types of Porosity – P‐Wave Velocity Relationships for the Nankai Trough
Authors: Yabe S. , Hamada Y. , Kitamura M. , Fukuchi R. , Hashimoto Y. 
Published: June 2022

AbstractThe empirical relationship between the porosity and P‐wave velocity is a useful tool for probing large‐scale underground physical properties and stress states based on P‐wave velocity structures acquired by seismic surveys. In this study, the porosity‐velocity curves were examined using local core sample and logging datasets acquired along the Kumano transect in the Nankai Trough, Southwest Japan. We tested Hashimoto et al.’s (2010) hypothesis that slope apron and accreted sediments have different relationships. Our advantage is using a large amount of logging and core data obtained at multiple sites in the Nankai Trough under various geological conditions, from the incoming oceanic plate to the inner wedge. We identified multiple types of porosity – P‐wave velocity relationships: 1) The first type agrees with the low‐velocity model in the global empirical relation of Erickson and Jarrard 1998), which is observed at lithostatic stress state condition (incoming upper Shikoku basin and slope sediments), 2) The second type consistent with high‐velocity models of the global empirical relation observed at compressive stress state condition (accreted sediments), which have a higher velocity and larger dependence on the porosity than the first type with the same porosity, and 3) The third type for incoming sediments with high smectite content has lower velocity than the first type. Based on our results, the transition between the first and second porosity – velocity relationships occurs in the prism toe, implying that compressive stress due to subduction controls acoustic properties and the lithification process of these sediments.
A biosensing system employing nanowell microelectrode arrays to record the intracellular potential of a single cardiomyocyte
Authors: Xiang Yuting , Liu Haitao , Yang Wenjian , Xu Zhongyuan , Wu Yue , Tang Zhaojian , Zhu Zhijing , Zeng Zhiyong , Wang Depeng , Wang Tianxing , Hu Ning , Zhang Diming 
Published: June 2022

AbstractElectrophysiological recording is a widely used method to investigate cardiovascular pathology, pharmacology and developmental biology. Microelectrode arrays record the electrical potential of cells in a minimally invasive and high-throughput way. However, commonly used microelectrode arrays primarily employ planar microelectrodes and cannot work in applications that require a recording of the intracellular action potential of a single cell. In this study, we proposed a novel measuring method that is able to record the intracellular action potential of a single cardiomyocyte by using a nanowell patterned microelectrode array (NWMEA). The NWMEA consists of five nanoscale wells at the center of each circular planar microelectrode. Biphasic pulse electroporation was applied to the NWMEA to penetrate the cardiomyocyte membrane, and the intracellular action potential was continuously recorded. The intracellular potential recording of cardiomyocytes by the NWMEA measured a potential signal with a higher quality (213.76 ± 25.85%), reduced noise root-mean-square (~33%), and higher signal-to-noise ratio (254.36 ± 12.61%) when compared to those of the extracellular recording. Compared to previously reported nanopillar microelectrodes, the NWMEA could ensure single cell electroporation and acquire high-quality action potential of cardiomyocytes with reduced fabrication processes. This NWMEA-based biosensing system is a promising tool to record the intracellular action potential of a single cell to broaden the usage of microelectrode arrays in electrophysiological investigation.
RhoA signaling increases mitophagy and protects cardiomyocytes against ischemia by stabilizing PINK1 protein and recruiting Parkin to mitochondria
Authors: Tu Michelle , Tan Valerie P. , Yu Justin D. , Tripathi Raghav , Bigham Zahna , Barlow Melissa , Smith Jeffrey M. , Brown Joan Heller , Miyamoto Shigeki 
Published: June 2022

AbstractMitophagy, a mitochondria-specific form of autophagy, removes dysfunctional mitochondria and is hence an essential process contributing to mitochondrial quality control. PTEN-induced kinase 1 (PINK1) and the E3 ubiquitin ligase Parkin are critical molecules involved in stress-induced mitophagy, but the intracellular signaling mechanisms by which this pathway is regulated are unclear. We tested the hypothesis that signaling through RhoA, a small GTPase, induces mitophagy via modulation of the PINK1/Parkin pathway as a protective mechanism against ischemic stress. We demonstrate that expression of constitutively active RhoA as well as sphingosine-1-phosphate induced activation of endogenous RhoA in cardiomyocytes result in an accumulation of PINK1 at mitochondria. This is accompanied by translocation of Parkin to mitochondria and ubiquitination of mitochondrial proteins leading to recognition of mitochondria by autophagosomes and their lysosomal degradation. Expression of RhoA in cardiomyocytes confers protection against ischemia, and this cardioprotection is attenuated by siRNA-mediated PINK1 knockdown. In vivo myocardial infarction elicits increases in mitochondrial PINK1, Parkin, and ubiquitinated mitochondrial proteins. AAV9-mediated RhoA expression potentiates these responses and a concurrent decrease in infarct size is observed. Interestingly, induction of mitochondrial PINK1 accumulation in response to RhoA signaling is neither mediated through its transcriptional upregulation nor dependent on depolarization of the mitochondrial membrane, the canonical mechanism for PINK1 accumulation. Instead, our results reveal that RhoA signaling inhibits PINK1 cleavage, thereby stabilizing PINK1 protein at mitochondria. We further show that active RhoA localizes at mitochondria and interacts with PINK1, and that the mitochondrial localization of RhoA is regulated by its downstream effector protein kinase D. These findings demonstrate that RhoA activation engages a unique mechanism to regulate PINK1 accumulation, induce mitophagy and protect against ischemic stress, and implicates regulation of RhoA signaling as a potential strategy to enhance mitophagy and confer protection under stress conditions.
β2-adrenergic receptor promotes liver regeneration partially through crosstalk with c-met
Authors: Tao Xiang , Chen Can , Chen Yingxiang , Zhang Luoying , Hu Jiong , Yu Hongjun , Liang Minglu , Fu Qin , Huang Kai 
Published: June 2022

AbstractThe β2-adrenergic receptor (β2AR) is a G protein-coupled receptor (GPCR) that mediates the majority of cellular responses to external stimuli. Aberrant expression of β2AR results in various pathophysiological disorders, including tumorigenesis, but little is known about its role in liver regeneration. This study aims to investigate the impact and the underlying mechanism of β2AR in liver regeneration. Here, we found that β2AR was upregulated during liver regeneration induced by 70% PH. Deletion of β2AR in mice resulted in 62% mortality 2 days post-PH, decreased proliferative marker expression and impaired liver function throughout regeneration. Moreover, AAV8-mediated overexpression of β2AR in hepatocytes accelerated the regeneration process and increased target gene expression. Mechanistically, β2AR recruited G-protein-coupled receptor kinase 2 (GRK2) to the membrane and then formed a complex with c-met to transactivate c-met signaling, which triggered downstream extracellular regulated protein kinase (ERK) signaling activation and nuclear translocation. Inhibition of c-met with SU11274 or ERK with U0126 decreased β2AR overexpression-induced hepatocyte proliferation. Our findings revealed that β2AR might act as a critical mediator regulating liver regeneration by crosstalk with c-met and activation of ERK signaling.
Estimation of the number of inherited prion disease mutation carriers in the UK
Authors: Corbie Rosie , Campbell Tracy , Darwent Lee , Rudge Peter , Collinge John , Mead Simon 
Published: June 2022

AbstractInherited prion diseases (IPD) are a set of rare neurodegenerative diseases that are always caused by mutation of the prion protein gene (PRNP). These are highly heterogeneous in clinical presentation and best described by the specific gene mutation, but traditionally include the canonical syndromes familial Creutzfeldt-Jakob disease, Gerstamann-Straussler-Scheinker syndrome, and fatal familial insomnia. In the UK, care of IPD patients and clinical PRNP sequencing have been carried out almost exclusively by the National Prion Clinic and affiliated laboratories since the disease gene was discovered in 1989. Using data obtained over 30 years (1990–2019), this study aimed to provide a greater understanding of the genetic prevalence of IPD using multiple complementary methods. A key source of bias in rare disorders is ascertainment, so we included an analysis based on capture-recapture techniques that may help to minimise ascertainment bias. 225 patients, with 21 different IPD mutations were identified, varying in frequency (with 8/21 mutations comprising over 90% observed cases), derived from 116 kindreds and 151 3-generation families. We estimated a total of 303 UK families (95% CI = 222, 384) segregate IPD mutations, 1091 (95% CI = 720, 1461) UK mutation carriers and a lifetime risk of approximately 1 in 60,000. Simpler methods of measuring prevalence based on extrapolation from the annual incidence of disease, and large scale genomic studies, result in similar estimates of prevalence. These estimates may be of value for planning preventive trials of therapeutics in IPD mutation carriers, prevention of prion disease transmission and provision of specialist services.