bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hydrodynamics of a tidal inlet under gray to green coastal protection interventions
Authors: Velasquez-Montoya Liliana , Wargula Anna , Nangle Jessica , Sciaudone Elizabeth , Smyre Elizabeth , Tomiczek Tori 
Published: October 2022

Structural coastal protection interventions have been implemented worldwide to stabilize channels and shorelines in tidal inlets. Although these conventional “gray” interventions typically attain their goals, there is an increasing interest and need to consider nature-based or “green” interventions that also address system resilience and environmental impacts. For a better implementation of gray to green interventions in tidal inlets, their effectiveness and their effects on the velocity of these dynamic coastal systems need to be understood. The effects on flow velocity of gray to green coastal protection interventions for tidal inlets are assessed here by exploring six interventions intended to protect against erosion on the estuarine-side shoreline near the inlet. A field-calibrated numerical model with Willmott Skill scores rated as excellent for Oregon Inlet located in North Carolina, United States, is used to simulate tidal currents under present conditions and after implementing a seawall, a set of bendway weirs, a terminal groin extension, a dual-jetty system, a flood channel relocation, and an island restoration project. Comparisons of time series of flow velocities in the flood channel along an eroding, estuarine shoreline are used to identify the effectiveness of each coastal protection alternative at reducing erosive velocities. Geospatial difference maps are used to determine velocity changes caused by each alternative throughout the inlet system. With no coastal protection interventions, the velocities along the eroding shoreline exceeded an erosive threshold velocity (defined as 0.2 m/s) during 50% of the simulated period. Alternatives closer to the green side of the coastal protection intervention spectrum, such as channel relocation and island restoration, tend to display the most effectiveness at reducing flow velocities at the eroding shoreline while resulting in minimal inlet-wide hydrodynamic changes. On the other hand, gray alternatives either cause minimal (seawall and bendway weirs) or extreme (jetties) changes in velocities throughout the inlet system. This comparison of gray and green coastal protection interventions in tidal inlets serves as an example to contrast the effectiveness of different coastal protection alternatives at reducing erosive flow velocities and to inform alternative selection at other inlet systems.
Assessing the impact of land use and changes in land cover related to carbon storage by linking trajectory analysis and InVEST models in the Nandu River Basin on Hainan Island in China
Authors: Gong Wenfeng , Duan Xuanyu , Mao Mingjiang , Hu Jihan , Sun Yuxin , Wu Genghong , Zhang Yangyang , Xie Yidan , Qiu Xincai , Rao Xiaodong , Liu Tiedong , Liu Tao 
Published: October 2022

This study aims to evaluate the effects of the spatiotemporal patterns of land-use and land-cover (LULC) changes on the dynamics of carbon storage in a tropical region of China by linking the trajectory analysis of LULC changes and the InVEST model. Based on remote sensing (RS), geographic information system (GIS) and change trajectories, the spatiotemporal evolution of LULC changes was explored. This evolution could be coupled with the spatiotemporal LULC change trajectories and the InVEST model for the quantitative study of the spatial distribution and temporal variation in regional carbon stocks. The results showed that during the 2000–2020 period, the built-up land continually increased to 206.05 km2 through urban expansion, and forestland became the dominant type of land, with an area of 357.39 km2. In addition to the change in land use, the carbon storage in the study region increased by 4.87 Tg C. The anaphasic trajectory had the largest area ratio at 7.05% in the total area, while the prophasic trajectory contributed to the largest increase in carbon storage, 5.87 Tg C. Moreover, the repetitive trajectories had no impact on carbon sinks and sources, whereas the anaphasic trajectory and the continual trajectory imposed passive impacts on carbon storage. These advances in research underpin scientific efforts to improve the understanding of the relationship between the optimization of land-use structure and patterns and the carbon storage service in the Nandu River Basin.
Lateral stress and its transmission law caused by operation of a double-wing subsoiler in sandy loam soil
Authors: Qin Kuan , Zhao Yun , Zhang Yongzheng , Cao Chengmao , Shen Zhougao 
Published: October 2022

Sandy loam soils are widespread and important for agricultural soil use. The lateral stress caused by the loosening shovel of a subsoiler produces lateral disturbances in sandy loam soil, reducing compaction and improving soil structure. To explore the variation and transmission of lateral stress due to the operation of a double-wing subsoiler in sandy loam soil, a lattice of point-type soil sensors was arranged in a soil bin, and the lateral stress generated by subsoiling was measured in the shallow, medium, and deep soil layers. The experimental results show that when the lateral stress generated by the double-wing subsoiler is transferred to the lateral position of different distances.The lateral stress shows a sinusoidal fluctuation law with time, the sine wave frequency range is: shallow (0.366–0.549); middle (0.306–0.494); and deep (0.088–0.501). There were greater fluctuation amplitudes in the transfer process for the shallow and deep soil layers than for the middle layer. Shallow soil had the largest final stable lateral stress (0.1617 N), and middle soil the smallest (0.0733 N). Thus, lateral stress from deep loosening has the greatest effect on shallow soil and the least effect on middle soil. These results indicate that the fluctuating lateral stresses generated by double-wing-subsoiler operation drive a fluctuating soil disturbance that can improve soil structure, and that they are greater for shallow and deep soils than for middle soils.
A comprehensive analysis of metabolomics and transcriptomics to reveal major metabolic pathways and potential biomarkers of human preeclampsia placenta
Authors: Feng Yan , Lian Xinlei , Guo Kaimin , Zhang Guanglan , Huang Xuan 
Published: October 2022

Background: The etiology of preeclampsia (PE) remains unclear. With the utilization of metabolomics, dysregulated production of several metabolic components in human plasma, such as lipids, amino acids, androgens and estrogens, was found to be important in the pathogenesis of PE. Transcriptomics adds more in-depth information, and the integration of transcriptomics and metabolomics may yield further insight into PE pathogenesis than either one alone.Objectives: We investigated the placental metabolomics and transcriptomics of PE patients to identify affected metabolic pathways and potential biological targets for exploring the disease pathogenesis.Methods: Integrated transcriptomics and metabolomics were used to analyze five paired human placentas from patients with severe PE and normal pregnancies. This was followed by further validation of our findings in a publicly available dataset of 173 PE vs. 157 control placentas. In addition, weighted gene coexpression network construction was performed to assess the correlation between genetic alterations and diseases.Results: We identified 66 and 41 differentially altered metabolites in negative and positive ion modes, respectively, in the PE group compared to the control group, and found 2,560 differentially expressed genes. Several pathways were aberrantly altered in the PE placenta at both the metabolic and transcriptional levels, including steroid hormone biosynthesis, the cAMP signaling pathway, neuroactive ligand–receptor interactions, taste transduction and prion diseases. Additionally, we found 11 differential metabolites and 11 differentially expressed genes involved in the steroid hormone biosynthesis pathway, indicating impaired metabolism of steroid hormones in the PE placenta. Furthermore, we found that CYP11A1, HSD3B2, and HSD17B6 are highly correlated with diseases.Conclusion: Our findings provide a profile of the dysregulated steroid hormone biosynthesis in PE placenta, we observed a dysregulated cortisol-to-cortisone ratio, testosterone accumulation, decreased testosterone downstream metabolites, impaired production of estrone and estriol, and aberrant hydroxylation and methylation of estradiol. Disorders of placental steroid hormone metabolism might be a consequence or a compensatory change in pathological placentation in PE, which underscores the need to investigate the physiology of steroid hormone metabolites in the etiology of PE.
SARS-CoV-2-Specific IgG and IgA response in maternal blood and breastmilk of vaccinated naïve and convalescent lactating participants
Authors: Longueira Yesica , Ojeda Diego S. , Battistelli Rocio B. Antivero , Sanchez Lautaro , Oviedo Rouco Santiago , Albano Daniel , Guevara Eleonora , Valls Vanesa , Pando María A. , Gamarnik Andrea V. 
Published: October 2022

BackgroundRecent studies have shown the presence of SARS-CoV-2-specific antibodies in the milk of breastfeeding mothers vaccinated with mRNA and convalescent. However, limited information is available in lactating women receiving other vaccine platforms used in developing countries, such as the inactivated SARS-CoV-2 vaccine BBIBP-CorV (Sinopharm) and the non-replicating adenovirus vaccines Sputnik V (Gamaleya Institute) and ChAdOx1-S (Oxford AstraZeneca).MethodsHere, we evaluated anti-SARS-CoV-2 IgG and IgA levels in both serum and milk samples using a longitudinal and a cross-sectional cohort of 208 breastfeeding vaccinated women from Argentina with or without previous SARS-CoV-2 infection.ResultsThe analysis showed that IgA levels remain constant in serum and milk of breastfeeding mothers between the first and second doses of vector-based vaccines (Sputnik V and ChAdOx1-S). After the second dose, anti-spike IgA was found positive in 100% of the serum samples and in 66% of breastmilk samples. In addition, no significant differences in milk IgA levels were observed in participants receiving BBIBP-CorV, Sputnik V or ChAdOx1-S. IgG levels in milk increased after the second dose of vector-based vaccines. Paired longitudinal samples taken at 45 and 120 days after the second dose showed a decrease in milk IgG levels over time. Study of IgA levels in serum and milk of vaccinated naïve of infection and vaccinated-convalescent breastfeeding participants showed significantly higher levels in vaccinated-convalescent than in participants without previous infection.ConclusionThis study is relevant to understand the protection against SARS-CoV-2 by passive immunity in newborns and children who are not yet eligible to receive vaccination.
Acute hypoxia changes the gene expression profiles and alternative splicing landscape in gills of spotted sea bass (Lateolabrax maculatus)
Authors: Ren Yuhang , Tian Yuan , Mao Xuebin , Wen Haishen , Qi Xin , Li Jinku , Li Jifang , Li Yun 
Published: October 2022

Hypoxia is one of the most important environmental stressors in aquatic ecosystems. To deal with the hypoxia environment, fishes exhibit a series of physiological and molecular responses to maintain homeostasis and organism functions. In the present study, hypoxia-induced changes in gene expression profiles and alternative splicing (AS) events in spotted sea bass (Lateolabrax maculatus), a promising marine-culture fish species in China, were thoroughly investigated by RNA-Seq analysis. A total of 1,242, 1,487 and 1,762 differentially expressed genes (DEGs) were identified at 3 h, 6 h and 12 h in gills after hypoxia stress. Functional enrichment analysis by KEGG and GSEA demonstrated that HIF signal network system was significantly activated and cell cycle process was remarkably suppressed in response to hypoxia. According to the temporal gene expression profiles, six clusters were generated and protein-protein interaction (PPI) networks were constructed for the two clusters that enriched with hypoxia-induced (cluster 2) or -suppressed genes (cluster 5), respectively. Results showed that HIF signaling related genes including vegfa, igf1, edn1, cox2b, cxcr4b, ctnnb1, and slc2a1a, were recognized as hubs in cluster 2, while mcm2, chek1, pole, mcm5, pola1, and rfc4, that tightly related to cell cycle, were down-regulated and considered as hubs in cluster 5. Furthermore, a total of 410 differential alternative splicing (DAS) genes were identified after hypoxia, which were closely associated with spliceosome. Of them, 63 DAS genes also showed differentially expressed levels after hypoxia, suggesting that their expression changes might be regulated by AS mechanism. This study revealed the key biological pathways and AS events affected by hypoxia, which would help us to better understand the molecular mechanisms of hypoxia response in spotted sea bass and other fish species.
Antimicrobial resistance of microorganisms present in periodontal diseases: A systematic review and meta-analysis
Authors: Abe Flávia Casale , Kodaira Katia , Motta Cristiane de Cássia Bergamaschi , Barberato-Filho Silvio , Silva Marcus Tolentino , Guimarães Caio Chaves , Martins Carolina Castro , Lopes Luciane Cruz 
Published: October 2022

ObjectiveThe aim of this study was to estimate the antimicrobial resistance in microorganisms present in periodontal diseases.MethodsA systematic review was conducted according to the PRISMA statement. The MEDLINE (PubMed/Ovid), EMBASE, BVS, CINAHL, and Web of Science databases were searched from January 2011 to December 2021 for observational studies which evaluated the antimicrobial resistance in periodontal diseases in permanent dentition. Studies that allowed the antimicrobial consumption until the time of sample collection, studies that used laboratory acquired strains, studies that only characterized the microbial strain present, assessment of cellular morphological changes, sequencing system validation, and time series were excluded. Six reviewers, working in pairs and independently, selected titles, abstracts, and full texts extracting data from all studies that met the eligibility criteria: characteristics of patients, diagnosis of infection, microbial species assessed, antimicrobial assessed, identification of resistance genes, and virulence factors. “The Joanna Briggs Institute” critical appraisal for case series was adapted to assess the risk of bias in the included studies.ResultsTwenty-four studies (N = 2.039 patients) were included. Prevotella and Porphyromonas species were the most cited microorganisms in the included studies, and the virulence factors were related to Staphylococcus aureus. The antimicrobial reported with the highest frequency of resistance in the included studies was ampicillin (39.5%) and ciprofloxacin showed the lowest frequency of resistance (3.4%). The most cited genes were related to macrolides. The quality of the included studies was considered critically low.ConclusionNo evidence was found regarding the profile of antimicrobial resistance in periodontal diseases, requiring further research that should focus on regional population studies to address this issue in the era of increasing antimicrobial resistance.Clinical relevanceThe knowledge about the present microorganism in periodontal diseases and their respective antimicrobial resistance profiles should guide dentists in prescribing complementary therapy for these infections.Systematic review registration[http://dx.doi.org/10.1097/MD.0000000000013158], identifier [CRD42018077810].
Efficacy of auricular plaster therapy for sleep disorders in preschool children with autism spectrum disorders: Study protocol for a randomized controlled trial
Authors: Duan Duoxi , He Lin , Chen Hong , Lei Ying , Wu Wei , Li Tao 
Published: October 2022

BackgroundChildren with autism spectrum disorders (ASDs) suffer from sleep disorders to a considerable degree; however, there is no safe and effective treatment available in clinical practice. The objective of the trial is to assess the clinical effectiveness of auricular plaster therapy (APT) in treating sleep disorders in children with ASD.MethodThis is a single-center, patient-assessor blind, randomized controlled trial. A total of 44 preschool children with sleep disorders with ASD will be included in this study. Eligible participants will be randomly assigned to either the auricular plaster group or the sham auricular plaster group in a 1:1 ratio. Participants in the different groups will receive APT or sham APT, respectively, for a total of 30 sessions over 30 days. The primary outcome includes the Children's Sleep Habits Questionnaire (CSHQ), while secondary outcomes include the Autism Behavior Checklist (ABC) and polysomnography (PSG) for total sleep time, sleep latency, awakening duration, and sleep structures. The CSHQ and ABC will be assessed at baseline, 10, 20, 30, 60, 90, and 120 days after randomization, whereas PSG will be assessed at baseline and 30 days after randomization. The follow-up period will be scheduled to be 60, 90, and 120 days after randomization.DiscussionThe results of this study may provide evidence of the efficacy of APT, as well as offer new alternatives for the treatment of sleep disorders in children with ASD.Trial registrationCHiCTR.org.cn (ChiCTR2100048257). Registered on July 5, 2021.
The effect of FTO rs9939609 polymorphism on the association between colorectal cancer and dietary fiber
Authors: Fathi Soroor , Ahmadzadeh Mina , Vahdat Mahsa , Afsharfar Maryam , Roumi Zahra , Hassanpour Ardekanizadeh Naeemeh , Shekari Soheila , Poorhosseini Seyed Mohammad , Gholamalizadeh Maryam , Abdollahi Sepideh , Kheyrani Elham , Doaei Saeid 
Published: October 2022

BackgroundGene polymorphisms may explain the controversy on the association between colorectal cancer (CRC) and dietary fibers. The purpose of this study was to investigate the effect of fat mass and obesity-associated (FTO) rs9939609 polymorphism on the association between colorectal cancer and dietary fiber.MethodsThis case-control study was conducted on 160 CRC cases and 320 healthy controls in Tehran, Iran. The participants' food intake was assessed using a semi-quantitative food frequency questionnaire (FFQ). The frequency of rs9939609 FTO polymorphism in the case and control groups was determined using the tetra-primer amplification refractory mutation (tetra-ARMS) method.ResultsIn the participants with the TT genotype of the FTO rs9939609, the cases had higher BMI and lower intake of dietary fiber compared to the controls (P = 0.01). Among A allele carriers of FTO rs9939609 polymorphism, the cases had higher BMI (P = 0.04) and lower intake of total fiber (P = 0.02) and soluble fiber (P = 0.02). An inverse association was found between CRC and dietary fiber intake among those with the AA/AT FTO rs9939609 genotype after adjusting for age, sex, smoking, alcohol consumption, physical activity, BMI, and calorie intake (OR = 0.9, CI 95%:0.84–0.92, P &lt; 0.05).ConclusionThis study found a link between higher dietary fiber consumption and a lower risk of CRC in A-allele carriers of FTO rs9939609 polymorphism. Future studies are needed to identify the underlying mechanisms of the association between CRC and dietary fibers in people with different FTO genotypes.
Breastfeeding performance index and associated factors among children aged 0–6 months in Ethiopia: Analysis of the 2019 Ethiopia Mini Demographic and Health Survey
Authors: Gessese Getachew Tilahun , Woldeamanuel Berhanu Teshome , Demie Takele Gezahegn , Diriba Biratu Tolesa , Handebo Simegnew 
Published: October 2022

BackgroundInfants under the age of 6 months are commonly affected by malnutrition globally. The higher the breastfeeding performance index (BPI), the greater the advantage of breastfeeding will be. However, there is a lack of literature in the context of Ethiopia. Therefore, this study is aimed at investigating the magnitude and determinants of the breastfeeding performance index score among mothers of children under the age of 6 months in Ethiopia.MethodsThis study was conducted using the 2019 Ethiopia Mini Demographic and Health Survey (EMDHS) dataset. A stratified, two-stage cluster sampling technique was used in the study. The survey data were weighted using the “svy” function in STATA version 16. Descriptive statistics, bivariable and multivariable logistic regression were employed in the analysis. An adjusted odds ratio (AOR) with a 95% confidence interval (CI) was reported. The results were considered statistically significant if the p-value was &lt; 0.05. The goodness of fit of the model was checked using the Hosmer–Lemeshow test.ResultsA total of 4,273 mothers with children under the age of 6 months were included in the analysis. Our analysis revealed that the prevalence of low breastfeeding performance index was 79.05% (95% CI: 78.01, 81.59). A unit increase in child age (AOR = 11.56; 95% CI: 6.97, 19.17), the richest wealth quintile (AOR = 2.76; 95% CI: 1.18, 6.5), a higher level of education (AOR = 5.41; 95% CI: 2.08, 14.05), being married or living with partner (AOR = 2.73; 95% CI: 1.18, 6.27), being women from Somali (AOR = 5.11; 95% CI: 2.08, 12.56), Afar (AOR = 3.03; 95% CI: 1.16, 7.91), Oromia (AOR = 1.88; 95% CI: 1.03, 3.41), Diredawa city administration (AOR = 2.89; 95% CI: 1.04, 8.07), and antenatal care (ANC) visit (AOR = 2.05; 95% CI: 1.31, 3.19) were positively associated with the low breastfeeding performance index.ConclusionThe prevalence of the low breastfeeding performance index was found to be high. Hence, the findings of the study suggest the need to target interventions aimed at improving breastfeeding performance toward mothers with higher socioeconomic and demographic status and educational status. Antenatal care clients are among the targets of the intervention.