bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of IMMS Containing Multi-site Liposomes for Dynamic Monitoring of
Blood CTC in Patients with Osimertinib-resistant Non-small-cell Lung Cancer and its
Mechanism
Authors: Li Bixun , Chen Xiaoju , Luo Ling , Lu Yanyan , Qiu Ye , Liang Jie , Chen Yulin , Ning Yufang 
Published: November 2022

Objective:
This article aims to establish a liquid biopsy system for gene detection of circulating tumor cells
(CTC) in lung cancer, systematically analyze the significance of osimertinib resistance, and formulate an individualized
diagnosis and treatment plan.

Methods:
Liposome-contained magnetic microspheres coated with Fe3O4 nanoparticles were synthesized by microemulsion,
and the surface was modified with EGFR antibody to form EGFR/EpCAM multi-site liposome-contained
immunomagnetic microspheres (IMMSs). The CTCs were isolated and identified from peripheral blood samples and
the cell lines of lung cancer patients collected by the multi-site liposome-contained IMMSs. To investigate the effects
of the order of use of IMMSs sequence at different sites on the sorting and trapping efficiency of non-small-cell lung
cancer (NSCLC) cells . The preliminary verification of drug-resistant gene function and dynamic monitoring of CTCs
in 20 patients with EGFR-positive NSCLC were screened and statistically analyzed before and after osimertinib treatment.
Sensitivity analysis and drug resistance evaluation of oxitidine were detected in vitro.

Results:
Results showed the prepared multi-site liposome-contained IMMSs had high stability and specificity. The
number of CTCs in blood samples of the patients with NSCLC was detected, revealing high sorting efficiency, and
positive sorting rate reaching more than 90%. We investigated the effect of osimertinib on the HER-2 expression on
the EGFR-mutated NSCLC cells and found that osimertinib increased the expression of HER-2 on the cell surface of
NSCLC cell lines., And further explored the therapeutic potential of osimertinib combined with T-DM1 at different
dosing times.

Conclusion:
Our results demonstrate that the prepared multi-site liposome-contained IMMSs can efficiently isolate
CTCs from the peripheral blood in lung cancer. Combined with the experimental data about osimertinib can be effectively
identified, the resistant genes of NSCLC including EGFR, which will provide a new scientific basis for guiding
clinical medication and formulating individualized treatment plans.
Application of Functional Bionic Technologies on Micropumps
Authors: Zhou Peijian , Wei Shang , Shu Lingfeng , Gao Shuyu , Mou Jiegang 
Published: November 2022

Background:
Bionics applied to micropumps is the most advanced technology currently accessible. The widespread use of microfluidic transport technology in fields like drug delivery and chemical analysis has made it a current research hotspot. As a core component in the microfluidic transport process, the micropump is a key part of the breakthrough.

Objective:
The study aims to summarize the engineering applications of various bionic micropumps in order to serve as a resource for future research in related fields.

Methods:
Study the application of bionic technologies that mimic fish tail fin oscillation, female mosquito blood sucking, honeybee nectar ingestion, and plant stomatal transpiration in various micropumps by sorting out typical research results.

Results:
This study examines the current state of bionic micropumps research and problems, as well as anticipates the future direction of functional bionic technology in micropumps.

Conclusion:
In this paper, we review the functional bionic technology used in micropumps and study some of the physiological processes of specific creatures from a biological perspective. We also show how effectively using bionic design can enhance the overall functionality of micropumps. However, man's knowledge of the natural world is still very limited. Functional bionics technology will shine in the area of engineering application as a result of the investigation of materials, processing technology, and biological principles.
The Research of Improved Mean-Shift Algorithm used in Rapid Localization
of Electromagnetic Objects under Specific Geographical Environment
Authors: hui Xue Guang , Wei Lv Jun 
Published: November 2022

Background:
The need for electromagnetic target positioning has grown increasingly
important in recent years, especially in the field of communication, navigation and other electromagnetic
fields.

Objective:
The objective of this study is to find a method for electromagnetic target location in different
geographical environment and improve the positioning accuracy of the electromagnetic target
in specific geographical environments effectively.

Methods:
This paper has proposed an improved Mean-shift Algorithm that can be used in the rapid
localization of electromagnetic objects under specific geographical environments. The paper also
analyses different interference source power prediction algorithms and gives out the analysis results
of the different algorithms. Parameter adjustments of mean shift algorithm are also given out, and
the related process in detail is presented.

Results:
Through the suppression jamming source location experiment, the proposed method can be
used in the rapid localization of electromagnetic objects under specific geographical environment,
and its characteristics are better than other localization algorithms.

Conclusion:
The proposed algorithm is suitable for the location of the electromagnetic target in a
specific geographical environment, and its accuracy is greatly improved compared with the traditional
method.
Amlodipine and Diltiazem Significantly Repress Human Rotavirus
Infection In Vitro
Authors: Tavakoli Ahmad , Khales Pegah , Keyvani Hossein , Ataei-Pirkooh Angila , Saghafi Mohammad Mehdi , Bokharaei-Salim Farah , Ghorbani Saied , Monavari Seyed Hamidreza , Kiani Seyed Jalal , Esghaei Maryam , Farahmand Mohammad , Sayyahfar Shirin , Khanaliha Khadijeh , Habib Zahra 
Published: November 2022

Background:
Considering the role of calcium in the replication and morphogenesis of rotaviruses, it is hypothesized that decreased cytosolic calcium levels by using calcium channel blockers can subsequently interfere with rotavirus replication.

Objective:
The present study investigated the effects of two calcium ion channel blockers, amlodipine and diltiazem, against human rotavirus infection.

Methods:
Cytotoxic effects of the drugs on MA-104 cells were evaluated using the neutral red assay. The effects of amlodipine and diltiazem at non-toxic concentrations on human rotavirus were examined using cytopathic effect inhibition, TCID50, and real-time PCR assays.

Results:
The highest inhibitory effect was obtained at concentrations of 0.5 μg/ml of amlodipine and 3 μg/ml of diltiazem, leading to 4.6 and 5.5 logarithmic reductions in infectious rotavirus titer and four- and a five-fold increase in the Ct values compared to the virus control, respectively (p-value <0.001). Conversely, infectious rotavirus titers were significantly elevated compared to the virus control at concentrations above 0.9 μg/ml of amlodipine and above 25 μg/ml of diltiazem.

Conclusion:
Our study suggests that in addition to cardiovascular diseases, calcium channel blockers at their optimal doses may also be used to treat gastroenteritis caused by rotavirus infection
Memorias del VI FORO DE EDUCACIÓN VETERINARIA
Authors: Facultades de Ciencias Veterinarias Federación panamericana de Escuelas y 
Published: November 2022

Durante el Congreso Panamericano de Ciencias Veterinarias (PANVET) celebrado en Santiago de Chile del 12 al 16 de noviembre de 2006, se convocó por primera vez al I Foro de Educación Veterinaria, espacio importante para difundir las experiencias y propuestas educativas en Medicina Veterinaria; fue tal la demanda, que desde entonces se ha celebrado el Foro de Educación Veterinaria ya que favorece la apertura de mejores y mayores posibilidades de intercambio científico, académico y social entre académicos e investigadores de las Ciencias Veterinarias de las Américas.
En esta ocasión, asociada a postpandemia por SARS-CoV-2, en su versión VI el Foro de Educación Veterinaria se desarrolló en la ciudad de Santiago de Chile, específicamente las puertas abiertas de la nueva Escuela de Ciencias Veterinarias de la Pontificia Universidad Católica de Chile fueron los anfitriones durante los días 24 al 26 de agosto del año 2022, en sus instalaciones se reunieron académicos líderes de procesos de investigación y docencia de 10 países de las Américas y se generaron un número importante de socializaciones (ponencias) de diversos temas de las Ciencias Veterinarias, además de mesas de trabajo en torno a temas vanguardistas de las ciencias Veterinarias como lo son la gestión de animales en desastres y la tendencia “One Health”, todo bajo el liderazgo del presidente de la Federación panamericana de Escuelas y Facultades de Ciencias Veterinarias, profesor Juan de Jesús Taylor Preciado.
DIFFICULTIES OF SPEAKING THAT ARE ENCOUNTERED BY ENGLISH LANGUAGE STUDENTS AT AL MARGEB UNIVERSITY
Authors: Dalem Mohamed 
Published: November 2022

The aim of this paper is to explore the speaking difficulties encountered by English language students at Al Margeb University, and to discover the causes of such difficulties. Speaking English has been a vital importance in international communication. Speaking fluent English is a common problem among the nonnative speakers. The second language learners have gone through a variety of cases to learn how to speak not only correct grammar and using the right vocabulary but with correct accent and pronunciation. Many obstacles, therefore, have been known as the predictors of such a problem among the language learners. According to the review of literature, appropriate speaking instruction was found to be the learners' priority and a field in which they need more attention. In this paper the writer highlight the speaking difficulties encountered by English language students at Al Margeb University, which are considered to be the most urgent for every teacher, such as fear of mistakes, shyness, anxiety, lack of confidence and lack of motivation. This paper can be useful to teachers to consider their language learners' speaking needs in English language teaching and learning context.
Game theoretic model for low carbon supply chain under carbon emissions reduction sensitive random demand
Authors: Ma Chao , Huang Fuyou , Hu Yongwen 
Published: November 2022

Abstract
This paper considers a low carbon supply chain consisting of a single manufacturer and a single retailer under the condition that the demand is uncertain. We first establish three games, including manufacturer Stackelberg (MS), retailer Stackelberg (RS) and Nash according to the different power structure of the firms. We then determine that the equilibrium stocking factors, emission reduction levels, wholesale prices and retail prices for the three models, respectively. After that, we demonstrate the effects of power structure. Results show that when the power shifts from the retailer to the manufacturer, the stocking factor decreases, whereas the wholesale price increases. Finally, we discuss the impacts of the random demand. We find that the expected profits of the firms, the emission reduction levels and the retail prices are increasing with respect to the market potential and low-carbon sensitivity coefficient, respectively. Meanwhile, they decrease with respect to the price sensitivity coefficient.
Impact Assessment of the Renewable Energy Policy Scenarios – a Case Study of Latvia
Authors: Kacare Marika , Pakere Ieva , Gravelsins Armands , Blumberga Andra 
Published: November 2022

Abstract
Even though the development of renewable energy technologies has been one of the most discussed and research-rich fields of science, and there are many practical and convincing technologies in the field of renewable energy, the path taken by society to shift from the use of non-renewable energy sources to the use of renewable ones has often been slow and unclear. Renewable energy technologies have undergone many improvements. There are several successful and promising examples where installing renewable energy technologies has paid off financially and improved the environment and quality of life. Nevertheless, fossil fuel still dominates or makes up a large proportion of energy production. The research simulates existing, planned, and potential policies to assess the best way to integrate renewable and local energy resources into the energy system by 2030 and in the long term. Policy analysis is carried out for several possible combinations of support measures to assess if it is possible to achieve the set targets in the National and Climate plan by 2030 and reach Climate neutrality by 2050. Such an approach makes it possible to assess the impact of existing policies that create synergies or undesirable side effects and whether they maximize the return on investment from a socio-economic and environmental point of view. In addition, a risk analysis and impact assessment of the proposed policy scenarios are carried out using multi-criteria analysis.
Endothelial dysfunction in patients with various forms of congenital epidermolysis bullosa
Authors: Kornev V. I. , Startseva O. N. , Pleshkov A. S. , Nikiforov M. V. 
Published: November 2022

Introduction. The endothelial system is an important component of vascular-platelet hemostasis, capable of actively responding to mechanical and inflammatory agents. Patients with congenital epidermolysis bullosa are prone to mechanical damage to the skin and the development of a chronic inflammatory syndrome with a high probability of endothelial dysfunction.The study objectivewas to assess the state of the endothelial system and to reveal the dependence of endothelial dysfunction on the form of epidermolysis bullosa.Methods and materials. The study used venous blood of 57 patients (27 men and 30 women) with congenital epidermolysis bullosa. In patients with simple and dystrophic forms of epidermolysis bullosa, the platelet count, P-selectin, fibrinogen, albumin, C-reactive protein, von Willebrand factor antigen concentration, and factor VIII activity were determined.Results. Comparative results of endothelial dysfunction depending on the form of epidermolysis bullosa were represented and endothelial dysfunction’s dependence on the concentration of albumin, C-reactive protein, and platelet count was determined.Conclusions. In patients with a dystrophic form of epidermolysis bullosa, endothelial dysfunction is accompanied by an increase in the expression of P-selectin, factor VIII activity, and the concentration of von Willebrand factor antigen. Chronic inflammation and impaired nutritional status with a decrease in albumin contribute to the development of endothelial dysfunction.
Systemic inflammation in salivary gland cancer
Authors: Kutukova S. I. , Belyak N. P. , Ivaskova J. V. , Razumova A. Ya. , Pakhomova N. V. , Frolov N. A. 
Published: November 2022

Relevance. The inflammatory process and endogenous intoxication of the patient’s body has a negative impact on the course of many malignant neoplasms, including salivary gland cancer.The objective of the study was to determine the influence of some factors of systemic inflammation and endogenous intoxication in salivary gland cancer.Methods and materials. A prospective study included the data of 59 patients with salivary gland cancer. The influence of peripheral blood parameters and relative indicators characterizing systemic inflammation on overall and disease-free survival was assessed.Results. As a result of the multivariate analysis, it was revealed that an increase in the level of the relative number of peripheral blood neutrophils by more than 60.08 % increases the risk of death in patients from salivary gland cancer by 3.90 times (p=0.0456; HR 3.90: 95 % CI 1.03–14.79). The level of the absolute number of peripheral blood lymphocytes, not exceeding 1.49x109 /l, increases the risk of disease progression by 8.72 times (p=0.0002, R 8.72: 95 % CI 2.78–27.28).Conclusion. Individual factors of systemic inflammation and endogenous intoxication, it is advisable to evaluate at the stage of planning the primary treatment of patients with salivary gland cancer, in order to determine the prognosis of the disease and optimize the choice of tactics for the primary treatment of patients.
Toxicity of Agave sisalana extracts on Cordyceps and their effect and the association with fungi on Nasutitermes corniger (Isoptera: Termitidae)
Authors: Da Silva Lopes Rosineide , Barroso Martins Mônica Cristina , De Lima Geiziquele , Gonçalves de Oliveira Luciana , Félix da Costa Antonio , Dos Santos Venézio Felipe , Dos Santos Correia Maria Tereza , Da Silva Nicácio Henrique , Correia de Albuquerque Auristela , Áurea de Luna Alves Lima Elza , De Menezes Lima Vera Lúcia 
Published: November 2022

Nasutitermes corniger is an arboreal termite that causes economic damage in urban areas, and its control is for chemical insecticides. An alternative for insect control is the use of entomopathogenic fungi and plant extracts, or the synergistic effect of the association of these. The toxicity of aqueous and hydroethanolic extracts of Agave sisalana on the biological aspects of the fungal Cordyceps farinosa, C. fumosorosea and C. javanica and the action of the extracts and their synergistic effect on N. corniger was examined. The effect of the extracts on fungi was evaluated by germination, sporulation and mycelial growth of fungi in Sabouraud medium through the study of biological aspects under laboratory conditions; and control of N. corniger were examined, by ingestion of extracts and of the association extract plus fungus on filter paper, being the percentage of mortality of soldiers and workers of the termite daily. In general, the extracts were inoffensive to the fungi at concentrations 10, 25, 50, 100 and 200 mg.mL-1. The extracts demonstrated termiticidal actions at concentrations tested, causing the mortalities about 100% of the workers after the third and sixth days (LC50: 0.778 to 0.803 mg.mL-1) and 100% of the soldiers between the fourth and seventh days (LC50:0.146 to 0.956 mg.mL-1). The association of the extracts with C. farinosa ESALQ1355 was more efficient in inducing the mortality in N. corniger. These results demonstrated the efficiency in vitro of the sisal extracts in controlling N. corniger termites, alone or in combination with fungi, suggesting their potential joint usefulness in the biological control of insect pests.
Criminal Competition: Spheres, Analysis and Counteraction in Public Administration
Authors: Стецюнич Юлия Николаевна , Зайцев Андрей Александрович 
Published: November 2022

Криминальная конкуренция - это элемент рыночных отношений, развитие которого представляет интерес для правоохранительных органов, поскольку оказывает отрицательное влияние на экономику в целом. В статье рассмотрена сущность понятия «криминальная конкуренция», выявлены отличия криминальной конкуренции от экономической (добросовестной) конкуренции, рассмотрены основные причины развития криминальной конкуренции в отечественной экономике, проанализированы статистические данные о составе преступлений в экономической сфере по причине криминальной конкуренции, в том числе в отдельных сферах деятельности, изучены действующие и предложены направления борьбы с недобросовестной конкуренцией в рамках государственного управления.
Criminal competition is an element of market relations representing interest to law enforcement agencies due to its negative impact on the economy as a whole. We consider the essence of criminal competition, identify its differences from economic (fair) competition and look at the main reasons for the development of criminal competition in the domestic economy. We also analyze the statistics concerning corpus delicti in economic sphere resulting from criminal competition in various areas of activity, make a research of current measures of unfair competition counteraction and suggest new ways of struggle against it within public administration.
Objectives, Purposes and Instruments of Implementation of «Science» National Project
Authors: Горчаков Сергей Евгеньевич 
Published: November 2022

В статье анализируются перспективы реализации национального проекта «Наука». Описываются ключевые цели проекта для научного и технологического обеспечения реализации задач и национальных приоритетов Российской Федерации, разработанных в рамках целеполагания на федеральном уровне и определенных в документах стратегического планирования. Подчеркнуто значение эффективной работы функциональной структуры в ходе инициирования, подготовки, реализации и завершения федеральных и региональных проектов и государственных программ. Обоснована необходимость совершенствования подходов в управлении реализацией национального проекта «Наука».
The article focuses on the prospects of implementation of «Science» national project. Particular attention is drawn to describing the key objectives of the project for the scientific and technological support aimed to ensure fulfillment of the aims and objectives of national priority which are developed within a goal setting framework at the federal level and which are defined in the relevant documents of strategic planning. The importance of efficient performance of functional structure is highlighted with regard to the processes of initiating, planning, implementing and completing the federal and regional projects along with government programs. The author substantiates the need for improving approaches and methods of «Science» national project management.