bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thoracoscopic posterior tracheopexy during primary esophageal atresia repair ameliorate tracheomalacia in neonates: a single-center retrospective comparative cohort study
Authors: Yasui Akihiro , Hinoki Akinari , Amano Hizuru , Shirota Chiyoe , Tainaka Takahisa , Sumida Wataru , Yokota Kazuki , Makita Satoshi , Okamoto Masamune , Takimoto Aitaro , Nakagawa Yoichi , Uchida Hiroo 
Published: July 2022

Abstract
Background
Esophageal atresia (EA) is often associated with tracheomalacia (TM). The severity of TM symptoms varies widely, with serious cases requiring prolonged respiratory support and surgical treatment. Although we performed thoracoscopic posterior tracheopexy (TPT) during primary EA repair to prevent or reduce the symptoms of TM, few studies have investigated the safety and effectiveness of TPT during primary EA repair. Therefore, this study aimed to evaluate the safety and efficacy of TPT in neonates.

Methods
We retrospectively reviewed the records of all patients diagnosed with TM who underwent primary thoracoscopic EA repair between 2013 and 2020 at the Nagoya University Hospital. Patients were divided into two groups: TPT (TPT group) and without TPT (control group). TPT has been performed in all patients with EA complicated by TM since 2020. We compared patient backgrounds, surgical outcomes, postoperative complications, and treatment efficacy.

Results
Of the 22 patients reviewed, eight were in the TPT group and 14 were in the control group. There were no statistically significant differences in the surgical outcomes between the groups (operation time: p = 0.31; blood loss: p = 0.83; time to extubation: p = 0.30; time to start enteral feeding: p = 0.19; time to start oral feeding: p = 0.43). Conversion to open thoracotomy was not performed in any case. The median operative time required for posterior tracheopexy was 10 (8–15) min. There were no statistically significant differences in postoperative complications between the groups (chylothorax: p = 0.36; leakage: p = 1.00; stricture: p = 0.53). The respiratory dependence rate 30 days postoperative (2 [25%] vs. 11 [79%], p = 0.03) and the ratio of the lateral and anterior–posterior diameter of the trachea (LAR) were significantly lower in the TPT group (1.83 [1.66–2.78] vs. 3.59 [1.80–7.70], p = 0.01).

Conclusions
TPT during primary EA repair for treatment of TM significantly lowered respiratory dependence rate at 30 days postoperative without increasing the risk of postoperative complications. This study suggested that TPT could improve TM associated with EA.
Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats
Authors: Wang Man , Geng Xufang , Li Kaipeng , Wang Yawen , Duan Xiaofeng , Hou Congcong , Zhao Lili , Zhou Huimin , Zhao Ding 
Published: July 2022

Abstract
Background
Berberine (BBR) has been found to have antiobesity effects, and obesity can lead to adipose tissue degeneration. As a special adipose tissue, perivascular adipose tissue (PVAT) is closely related to vascular function and affects vasoconstriction and relaxation. What happens to PVAT in the early stages of diet-induced obesity and how BBR affects vascular function is the focus of our experimental study.

Methods
Sprague–Dawley rats were fed a high-fat diet (fat 34% kcal) for 4 weeks to simulate early obesity. Obese rats were treated with BBR (200 mg/kg) or metformin (MET, 100 mg/kg) by gavage for 2 weeks. The mesenteric arterioles were studied by atomic force microscopy (AFM). The force vs. time curves were observed and analysed to indicate vascular function. Nitric oxide (NO) and noradrenaline (NA) release was quantified using an organ bath with fluorescence assays and ELISA, respectively. Network pharmacology was used to analyse the overlapping targets related to BBR and obesity-related diseases, and the expression of NOS in mesenteric PVAT was further analysed with immunohistochemistry and real-time PCR. The serum inflammatory factor levels were tested.

Results
BBR significantly reduced the levels of blood glucose, blood lipids and inflammatory factors in serum. It also effectively improved abnormal mesenteric vasoconstriction and relaxation in obese rats. There was no significant change in mesenteric vascular structure, but NO production and eNOS expression were significantly increased in mesenteric PVAT (P < 0.01), and NA was decreased (P < 0.05) in obese rats. All these changes in the mesenteric arterioles and PVAT of obese rats were reversed by treatment with BBR and MET.

Conclusions
In diet-induced obesity in rats, the function of vasoconstriction and relaxation in mesenteric arterioles is altered, NO is increased, and NA is decreased in mesenteric PVAT. All these changes were reversed by BBR, suggesting a novel effect of BBR in ameliorating mesenteric vascular dysfunction by regulating PVAT.
Difference between self-reported adherence to standard precautions and surveillance and factors influencing observed adherence: a quantile regression approach
Authors: Kim Jin Suk , Lee Eunhee 
Published: July 2022

Abstract
Background
Standard precautions are minimum healthcare-associated infection prevention practices applied in all healthcare settings. The aim of this study was to investigate adherence to standard precautions using a survey and surveillance. Factors affecting observed adherence to standard precautions were also determined.

Methods
This cross-sectional observational study included 163 clinical nurses who were directly involved in patient care. Differences in adherence according to investigative methods are represented as a boxplot. Quantile regression was used to identify factors affecting observed adherence, including organizational factors (such as department, safety environment, and patient safety climate) and personal factors (such as knowledge and awareness). Stata SE version 14.2 was used for all statistical analyses.

Results
The observed adherence to standard precautions was 76.8 out of 100, whereas the self-reported adherence was approximately 95. Hand hygiene adherence received the lowest score of less than 70. Factors influencing observed adherence were self-reported adherence (p = 0.043) in 25% and 50% quantiles, work experience (p = 0.002) in the 25% quantile, and working department (p = 0.030) in the 50% quantile. There were no significant factors in the 75% quantile.

Conclusion
Inadequate adherence to standard precautions might increase healthcare-associated infections. Thus, an organizational environment such as nurse staffing needs to be established so that clinical nurses with high competency can comply with standard precautions in clinical settings.
Can we scale up a comprehensive school-based eye health programme in Zambia?
Authors: Yong Ai Chee , Buglass Anne , Mwelwa Godfrey , Abdallah Ibrahim , Chan Ving Fai 
Published: July 2022

Abstract
Background
Globally, 19 million children have preventable vision impairment simply because refractive and eye health services are inaccessible to most of them. In Zambia, approximately 50,000 school children need spectacle provision. The School-based Eye Health Programme (SEHP) has been identified worldwide as a proven strategy to address childhood blindness. Given its great benefits, the Zambian government intends to scale up the programme. This scalability assessment aims to identify and evaluate the essential components of an effective SEHP, determine roles, assess existing capacities within user organisations, identify environmental facilitating and inhibiting factors, and estimate the minimum resources necessary for the scaling up and their proposed scale-up strategies.

Methods
Five elements (innovation, user organisation, resource team, environment, and strategies for horizontal and vertical scaling-up) were assessed guided by the ExpandNet-WHO Nine Steps for Developing a Scaling-Up Strategy. Literature review on proven strategies to reduce childhood blindness and the credibility of SEHP implemented in resource-limited settings, document review on the pilot project, questionnaires, and stakeholders’ interviews were conducted to collect data for this assessment. Subsequently, twenty questions in the Worksheets for Developing a Scaling-up Strategy were used to report the assessment outcome systematically.

Results
Additional components of SEHP incorporated in Zambia’s model enhanced the innovation’s credibility and relevance. The resource team was relatively competent in the pilot project, and the same team will be employed during the scaling-up. Potential change in political parties, the lack of supply chain, and unstable financial support were identified as inhibiting factors. The objectives of SEHP were aligned with the National Eye Health Strategic Plan 2017–2021, which supports the institutionalisation of the SEHP into the existing School Health and Nutrition Programme. For the pace of expansion, replicating SEHP to another district rather than a province will be more realistic.

Conclusion
Scaling up a comprehensive SEHP in Zambia is feasible if sufficient funding is available. Additionally, the pace must be adapted to the local context to ensure that every component within the SEHP is intact.
STAT3-mediated osteogenesis and osteoclastogenesis in osteoporosis
Authors: Hou Xiaoli , Tian Faming 
Published: July 2022

AbstractOsteoporosis is a common skeletal disease with marked bone loss, deterioration of the bone microstructure and bone fragility. An abnormal bone remodelling cycle with relatively increased bone resorption is the crucial pathophysiological mechanism. Bone remodelling is predominantly controlled by osteoblasts and osteoclasts, which are specialized cell types that are regulated by a variety of osteogenic and osteoclastic factors, including cytokines expressed within the bone microenvironment under local or systemic inflammatory conditions. Signal transducer and activator of transcription 3 (STAT3) plays a prominent role in the communication between cytokines and kinases by binding downstream gene promotors and is involved in a wide range of biological or pathological processes. Emerging evidence suggests that STAT3 and its network participate in bone remodelling and the development of osteoporosis, and this factor may be a potent target for osteoporosis treatment. This review focuses on the role and molecular mechanism of the STAT3 signalling pathway in osteogenesis, osteoclastogenesis and osteoporosis, particularly the bone-related cytokines that regulate the osteoblastic differentiation of bone marrow stromal cells and the osteoclastic differentiation of bone marrow macrophages by initiating STAT3 signalling. This review also examines the cellular interactions among immune cells, haematopoietic cells and osteoblastic/osteoclastic cells.
cGAS inhibition alleviates Alu RNA-induced immune responses and cytotoxicity in retinal pigmented epithelium
Authors: Li Jing , Zhang Feng , Bian Wei , Chen Yanyun , Liu Jianying , Liu Zhenyu , Xiong Ying , Wan Xiuhua 
Published: July 2022

Abstract
Background
The degeneration of retinal pigmented epithelium (RPE) cells results in severe diseases, such as age-related macular degeneration (AMD) that causes blindness in millions of individuals.

Results
We report that targeting GMP-AMP (cGAMP) synthase (cGAS) alleviates Alu RNA-induced immune responses and cytotoxicity in RPE. We find that the deletion of cGAS in RPE inhibits the Alu RNA-stimulated interferon production. cGAS deficiency also protects RPE from cell death triggered by Alu RNA. Importantly, two natural chemicals, epigallocatechin gallate (EGCG) and resveratrol (RSVL), are effective in suppressing the immunogenic and cytotoxic effect of Alu RNA in RPE.

Conclusions
Our findings further demonstrate the crucial role of cGAS in the Alu RNA-induced RPE damage and present EGCG and RSVL as potential therapies for AMD and other RPE degeneration-related conditions.
Comparative analysis of human and bovine thyroglobulin structures
Authors: Kim Han-ul , Jeong Hyeongseop , Chung Jeong Min , Jeoung Dooil , Hyun Jaekyung , Jung Hyun Suk 
Published: July 2022

AbstractIn biology, evolutionary conserved protein sequences show homologous physiological phenotypes in their structures and functions. If the protein has a vital function, its sequence is usually conserved across the species. However, in highly conserved protein there still remains small differences across the species. Upon protein–protein interaction (PPI), it is observed that the conserved proteins can have different binding partners that are considered to be caused by the small sequence variations in a specific domain. Thyroglobulin (TG) is the most commonly found protein in the thyroid gland of vertebrates and serves as the precursor of the thyroid hormones, tetraiodothyronine and triiodothyronine that are critical for growth, development and metabolism in vertebrates. In this study, we comparatively analyzed the sequences and structures of the highly conserved regions of TG from two different species in relation to their PPIs. In order to do so, we employed SIM for sequence alignment, STRING for PPI analysis and cryo-electron microscopy for 3D structural analysis. Our Cryo-EM model for TG of Bos taurus determined at 7.1 Å resolution fitted well with the previously published Cryo-EM model for Homo sapiens TG. By demonstrating overall structural homology between TGs from different species, we address that local amino acid sequence variation is sufficient to alter PPIs specific for the organism. We predict that our result will contribute to a deeper understanding in the evolutionary pattern applicable to many other proteins.
A broadband magnetotelluric survey for Mt. Meakandake volcano with special attention to the unrest during 2016–2017
Authors: Inoue Tomohiro , Hashimoto Takeshi , Tanaka Ryo , Yamaya Yusuke 
Published: July 2022

AbstractWe performed a broadband magnetotelluric (BBMT) survey and three-dimensional resistivity modeling for the Meakandake volcano in eastern Hokkaido, Japan, where remarkable ground deformation suggests a sill-like intrusion on the northeastern flank from 2016 to 2017. The volcano remained unerupted, and therefore the volcanological meaning of the deformation was unclear, making the evaluation of the “unrest” event difficult. Our 3D MT model has revealed a subvertical low-resistivity column C1 (approximately 1–10 Ωm) extending from 0.5 km BSL (below sea level) to a deeper part of Mt. Meakandake. The conductor C1 was not right on the presumed sill but just beneath the summit craters offset southwest. We performed a sensitivity test in which the bottom limit of C1 was varied, and confirmed that C1 was meaningful down to approximately 30 km BSL. The vertical reach in depth was necessary to reproduce the impedance phases out of quadrant at some sites west of Mt. Meakandake. In addition, we interpreted that the uppermost part of C1 was probably connected to the active vents of Mt. Meakandake through presumed subvertical pathways of heat and fluids, corresponding to the alignment of microearthquakes. On the other hand, we found no remarkable conductivity anomalies beneath the northeastern flank, where the sill-like inflation source was presumed. While our MT data do not suggest a thin sill at depth, it does not exclude the possibility that the ground inflation has been caused by a lateral magmatic or hydrothermal intrusion that branched from a certain depth of the subvertical conductor C1.
Graphical Abstract
The habit, choice, intention, and perception of raw beef consumers on raw beef-eating: the health risk management perspective
Authors: Gebeyehu Daniel Teshome , Alemu Biruk , Belete Gemechu 
Published: July 2022

AbstractApart from its nutritive value, meat is one of the substances for the transmission of pathogenic micro-organisms to consumers and the raw beef eating habit of Ethiopians can create a favourable condition for the transmission of pathogens from contaminated meat to raw beef consumers. The face-to-face interview of raw beef consumers was done using a structured questionnaire and 570 total samples were collected. A considerable number (74%) of raw beef consumers had favourable food choice; 85% of the raw beef consumers had favourable intentions to stop their raw beef eating habit, and 67% of them had an unfavourable perception of the safety of raw beef-eating. In conclusion, the study showed that raw beef consumers were not aware of the health risks of raw beef-eating. As a result, urgent sensitization intervention is required to shift the raw beef consumers from unhealthy eating habits to prudent (processed) eating practices.
Students' self-repair in EFL classroom interactions: implications for classroom dynamics
Authors: Beshir Mohammed , Yigzaw Abiy 
Published: July 2022

AbstractThis study aimed to evaluate whether or not students engage in self-help behavior when they encounter difficulties during their presentations. The participants were second year EFL college trainees; the data were obtained from their classroom presentations. The audio recorded data has been transcribed, coded, and categorized into quantitative and qualitative data. Descriptive statistical tools were employed to analyze the quantitative component, while thematic analysis was used to explain the qualitative data. Based on the findings, the students engaged in different kinds of strategies for self-repair, including same-information repair (repetition) 48 (36.9%); appropriateness repair 46 (35.4%); error repair 32 (24.6%); and back-to-error 4 (3.1%). From the point of view of the trouble sources that trigger self-repair, this research highlighted difficulties with syntactic and lexical errors as the most prevalent problems among EFL learners. The self-repair evidences revealed the importance of giving time for students to modify their utterances by themselves. In addition, the result could help teachers determine where to focus their efforts in helping students.
A review of real-time multi-GNSS precise orbit determination based on the filter method
Authors: Lou Yidong , Dai Xiaolei , Gong Xiaopeng , Li Chenglong , Qing Yun , Liu Yang , Peng Yaquan , Gu Shengfeng 
Published: July 2022

AbstractStable and reliable high-precision satellite orbit products are the prerequisites for the positioning services with high performance. In general, the positioning accuracy depends strongly on the quality of satellite orbit and clock products, especially for absolute positioning modes, such as Precise Point Positioning (PPP). With the development of real-time services, real-time Precise Orbit Determination (POD) is indispensable and mainly includes two methods: the ultra-rapid orbit prediction and the real-time filtering orbit determination. The real-time filtering method has a great potential to obtain more stable and reliable products than the ultra-rapid orbit prediction method and thus has attracted increasing attention in commercial companies and research institutes. However, several key issues should be resolved, including the refinement of satellite dynamic stochastic models, adaptive filtering for irregular satellite motions, rapid convergence, and real-time Ambiguity Resolution (AR). This paper reviews and summarizes the current research progress in real-time filtering POD with a focus on the aforementioned issues. In addition, the real-time filtering orbit determination software developed by our group is introduced, and some of the latest results are evaluated. The Three-Dimensional (3D) real-time orbit accuracy of GPS and Galileo satellites is better than 5 cm with AR. In terms of the convergence time and accuracy of kinematic PPP AR, the better performance of the filter orbit products is validated compared to the ultra-rapid orbit products.
A Population-Based Study of the Long-Term Risk of Infections Associated With Hospitalization in Childhood Cancer Survivors
Authors: Chehab Leena , Doody David R. , Esbenshade Adam J. , Guilcher Gregory M.T. , Dvorak Christopher C. , Fisher Brian T. , Mueller Beth A. , Chow Eric J. , Rossoff Jenna 
Published: July 2022

PURPOSE Infections pose a significant risk during therapy for childhood cancer. However, little is known about the risk of infection in long-term survivors of childhood cancer. METHODS We performed a retrospective observational study of children and adolescents born in Washington State diagnosed with cancer before age 20 years and who survived at least 5 years after diagnosis. Survivors were categorized as having a hematologic or nonhematologic malignancy and were matched to individuals without cancer in the state birth records by birth year and sex with a comparator:survivor ratio of 10:1. The primary outcome was incidence of any infection associated with a hospitalization using diagnostic codes from state hospital discharge records. Incidence was reported as a rate (IR) per 1,000 person-years. Multivariate Poisson regression was used to calculate incidence rate ratios (IRR) for cancer survivors versus comparators. RESULTS On the basis of 382 infection events among 3,152 survivors and 771 events among 31,519 comparators, the IR of all hospitalized infections starting 5 years after cancer diagnosis was 12.6 (95% CI, 11.4 to 13.9) and 2.4 (95% CI, 2.3 to 2.6), respectively, with an IRR 5.1 (95% CI, 4.5 to 5.8). The survivor IR during the 5- to 10-year (18.1, 95% CI, 15.9 to 20.5) and > 10-year postcancer diagnosis (8.3, 95% CI, 7.0 to 9.7) periods remained greater than comparison group IRs for the same time periods (2.3, 95% CI, 2.1 to 2.6 and 2.5, 95% CI, 2.3 to 2.8, respectively). When potentially vaccine-preventable infections were evaluated, survivors had a greater risk of infection relative to comparators (IRR, 13.1; 95% CI, 7.2 to 23.9). CONCLUSION Infectious complications continue to affect survivors of childhood cancer many years after initial diagnosis. Future studies are needed to better understand immune reconstitution to determine specific factors that may mitigate this risk.
Disrupted and Elevated Circadian Secretion of Glucagon-Like Peptide-1 in a Murine Model of Type 2 Diabetes
Authors: Biancolin Andrew D , Jeong Hyerin , Mak Kimberly W Y , Yuan Zixuan , Brubaker Patricia L 
Published: July 2022

AbstractMetabolism and circadian rhythms are intimately linked, with circadian glucagon-like peptide-1 (GLP-1) secretion by the intestinal L-cell entraining rhythmic insulin release. GLP-1 secretion has been explored in the context of obesogenic diets, but never in a rodent model of type 2 diabetes (T2D). There is also considerable disagreement regarding GLP-1 levels in human T2D. Furthermore, recent evidence has demonstrated decreased expression of the β-cell exocytotic protein secretagogin (SCGN) in T2D. To extend these findings to the L-cell, we administered oral glucose tolerance tests at 6 time points in 4-hour intervals to the high-fat diet/streptozotocin (HFD-STZ) mouse model of T2D. This revealed a 10-fold increase in peak GLP-1 secretion with a phase shift of the peak from the normal feeding period into the fasting-phase. This was accompanied by impairments in the rhythms of glucose, glucagon, mucosal clock genes (Arntl and Cry2), and Scgn. Immunostaining revealed that L-cell GLP-1 intensity was increased in the HFD-STZ model, as was the proportion of L-cells that expressed SCGN; however, this was not found in L-cells from humans with T2D, which exhibited decreased GLP-1 staining but maintained their SCGN expression. Gcg expression in isolated L-cells was increased along with pathways relating to GLP-1 secretion and electron transport chain activity in the HFD-STZ condition. Further investigation into the mechanisms responsible for this increase in GLP-1 secretion may give insights into therapies directed toward upregulating endogenous GLP-1 secretion.