bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular mechanisms involved in Atlantic halibut (Hippoglossus hippoglossus) egg quality: impairments at transcription and protein folding levels induce inefficient protein and energy homeostasis during early development
Authors: Yilmaz Ozlem , Jensen Anders Mangor , Harboe Torstein , Møgster Margareth , Jensen Ragnfrid Mangor , Mjaavatten Olav , Birkeland Even , Spriet Endy , Sandven Linda , Furmanek Tomasz , Berven Frode S. , Wargelius Anna , Norberg Birgitta 
Published: February 2022

ABSTRACTBackgroundReproductive success and normal development in all animals are dependent on egg quality and developmental competence of the produced embryo. This study employed tandem mass tags labeling based liquid chromatography tandem mass spectrometry for egg proteomic profiling to investigate differences in the global proteome of good versus poor quality Atlantic halibut eggs at 1-cell stage post fertilization.ResultsA total of 115 proteins were found to be differentially abundant between good and poor quality eggs. Frequency distribution of these proteins revealed higher protein folding activity in good quality eggs in comparison to higher transcription and protein degradation activities in poor quality eggs (p< 0.05). Poor quality halibut eggs were significantly enriched with additional proteins related to mitochondrial structure and biogenesis (p< 0.05). The differential abundance of a selection of proteins was first confirmed at gene expression level using a transcriptomic approach followed by a targeted proteomic approach (parallel reaction monitoring based mass spectrometry) in biological samples obtained from two consecutive reproductive seasons. The findings of global proteome profiling, together with the validation of differential abundance of targeted proteins and their related genes, suggest impairments in protein and energy homeostasis which might be related to unfolded protein response and mitochondrial stress in poor quality eggs. Additional transmission electron microscopy studies were taken to assess potential differences in abundance and morphological integrity of mitochondria between good and poor quality eggs. Observations reveal poor quality eggs to contain significantly higher number of mitochondria with higher number of cristae. These mitochondria, however, are significantly smaller and have a more irregular shape than those found in high-quality eggs. Therewithal difference in mtDNA levels represented bymt-nd5andmt-atp6genomic DNA abundance in this study, were found to be not statistically significant (p> 0.05) between good and bad quality eggs at both 1 hpf and 24 hpf stages.ConclusionOverall evidence from this study indicate that poor quality eggs undergo impairments at both transcription and translation level leading to endoplasmic reticulum and mitochondrial deficiencies. Additional research may be required to expediate the details and the potential of these impairments occurring in different species. Nonetheless, this study will pave the way for future research and will help in acceleration of recent advances in the field of embryonic developmental competence of living organisms.
Identification of interacting proteins of maize mosaic virus glycoprotein in its vector, Peregrinus maidis
Authors: Barandoc-Alviar Karen , Rotenberg Dorith , Martin Kathleen M. , Whitfield Anna E. 
Published: February 2022

AbstractRhabdovirus glycoproteins (G) serve multifunctional roles in virus entry, assembly, and exit from animal cells. We hypothesize that maize mosaic virus (MMV) G is required for invasion, infection, and spread in Peregrinus maidis, the planthopper vector. Using a membrane-based yeast two-hybrid assay, we identified 125 P. maidis proteins that physically interacted with MMV G, of which 68% matched proteins with known functions in endocytosis, vesicle-mediated transport, protein synthesis and turnover, nuclear import/export, metabolism and host defense. Physical interaction networks among conserved proteins indicated a possible cellular coordination of processes associated with MMV G translation, protein folding and trafficking. Non-annotated proteins contained predicted functional sites, including a diverse array of ligand binding sites. Cyclophilin A and apolipophorin III co-immunoprecipitated with MMV G, and each showed different patterns of co-localization with G in insect cells. This study describes the first protein interactome for a rhabdovirus spike protein and insect vector.
A widespread group of large plasmids in methanotrophic Methanoperedens archaea
Authors: Schoelmerich Marie C. , Oubouter Heleen T. , Sachdeva Rohan , Penev Petar , Amano Yuki , West-Roberts Jacob , Welte Cornelia U. , Banfield Jillian F. 
Published: February 2022

AbstractAnaerobic methanotrophic (ANME) archaea conserve energy from the breakdown of methane, an important driver of global warming, yet the extrachromosomal genetic elements that impact the activities of ANME archaea are little understood. Here we describe large plasmids associated with ANME archaea of the Methanoperedens genus. These have been maintained in two bioreactors that contain enrichment cultures dominated by different Methanoperedens species and co-occur with Methanoperedens species in other anoxic environments. By manual curation we show that two of the plasmids are large (155,607 bp and 191,912 bp), circular, and replicate bidirectionally. The group of Methanoperedens species that carry these plasmids is related to “Ca. Methanoperedens nitroreducens”, “Ca. Methanoperedens ferrireducens”, “Ca. Methanoperedens manganicus" and the plasmids occur in the same copy number as the main chromosome. The larger plasmid encodes transporters that potentially enhance access to Ni, which is required for the methyl-CoM reductase (Mcr), Co required for the cobalamin cofactor needed for methyltransferases, and amino acid uptake. We show that many plasmid genes are actively transcribed, including genes involved in plasmid chromosome maintenance and segregation, a Co2+/Ni2+ transporter and cell protective proteins. Notably, one plasmid carries three tRNAs and two colocalized genes encoding ribosomal protein uL16 and elongation factor eEF2. These are not encoded in the host Methanoperedens genome and uL16 and eEF2 were highly expressed, indicating an obligate interdependence between this plasmid and its host. The finding of plasmids of Methanoperedens opens the way for the development of genetic vectors that could be used to probe little understood aspects of Methanoperedens physiology. Ultimately, this may provide a route to introduce or alter genes that may enhance growth and overall metabolism to accelerate methane oxidation rates.
Real-life performance of a COVID-19 rapid antigen detection test targeting the SARS-CoV-2 nucleoprotein for diagnosis of COVID-19 due to the Omicron variant
Authors: Michelena Paula de , Torres Ignacio , Ramos-García Ángela , Gosalbes Victoria , Ruiz Nidia , Sanmartín Ana , Botija Pilar , Poujois Sandrine , Huntley Dixie , Albert Eliseo , Navarro David 
Published: February 2022

ABSTRACTObjectivesIt has been suggested that rapid antigen detection assays (RADT) may perform suboptimally in terms of sensitivity for the diagnosis of SARS-CoV-2 Omicron variant infection. To address this issue, we conducted a prospective study in primary health centers to evaluate the clinical performance of the Panbio™ COVID-19 Ag Rapid Test Device in nasopharyngeal specimens (NP) carried out at the point of care.MethodsWe recruited 244 patients (median age, 40 years; range 2–96; 141 female) with clinical suspicion of COVID-19 (232 adults and 12 children). 228/244 patients had been fully vaccinated (two doses) with licensed COVID-19 vaccines prior to recruitment. Most patients (222/244) were SARS-CoV-2 naïve prior to enrollment. Patients were tested by RT-PCR and RADT within 5 days since symptoms onset.Results126 patients (51.6%) tested positive by both RT-PCR and RADT, 90 patients (36.8%) returned negative results by both assays and 28 patients (11.4%) yielded discordant results (RT-PCR+/RADT-). No patients tested RT-PCR-/RADT+. Overall specificity and sensitivity of RADT was 100% (95% CI, 95.9–100%) and 81.8% (95% CI, 75–87.1%) respectively. The sensitivity of the assay increased from 79.6% (95% CI, 66.4–88.5) when considering specimens collected at days 0–1 after symptoms onset, to 86.4% (95% CI, 66.7–95.3) when grouping the specimens obtained on days 4–5.ConclusionThe Panbio™ COVID-19 Ag Rapid Test Device perform well (≥80% sensitivity) as a point-of-care test for early diagnosis of COVID-19 due to the Omicron variant in primary healthcare centers.
Research on the influencing factors of knowledge transfer among construction workers based on social cognitive theory
Authors: Zhou Shan , Qin Luping , Zhang Jixuan , Cao Xinying 
Published: February 2022

PurposeThis paper aims to employ social cognitive theory (SCT) as a theoretical framework to investigate the influencing factors affecting the knowledge transfer effectiveness of construction workers in China. The mediating role of their knowledge transfer willingness is also assessed.Design/methodology/approachA questionnaire on knowledge transfer among Chinese construction workers is designed and subsequently analyzed by structural equation modeling (SEM), with a total sample of 288 construction workers.FindingsThe SEM results show that the knowledge self-efficiency, blood and geographic relationships, and trust relationship promote knowledge transfer willingness and positively influence the knowledge transfer effectiveness of construction workers. However, the effect of organizational culture on knowledge transfer willingness and effectiveness is nonsignificant.Practical implicationsThe results are conducive to managers and governments formulating strategies to optimize the learning mechanism of construction workers and facilitate their obtaining of resources from the project, thus easing skill shortages and promoting the transformation of construction workers into industrial workers.Originality/valueThis paper innovatively proposes blood and geographic relationships as research variables, expanding their scope. Furthermore, SCT is applied to enable future research to better understand individual knowledge transfer behavior from both personal and environmental perspectives.
Top management team gender diversity and firm risk-taking in the hospitality industry
Authors: Ozdemir Ozgur , Erkmen Ezgi 
Published: February 2022

PurposeThe purpose of this study is to investigate the link between top management team (TMT) gender diversity and firm risk-taking in hospitality companies. The study also links female leadership to risk-taking. Finally, this study examines the moderating effects of TMT incentive pay and TMT age on the relationship between TMT gender diversity and firm risk-taking.Design/methodology/approachThis study uses an unbalanced data set of 81 hospitality firms and 888 firm-year observations over the period of 1992–2020. The study uses fixed-effects regression estimation for primary analyses and addresses potential endogeneity concerns via two-stage least square regression with firm fixed-effects instrumental variable regression. Risk-taking is measured by total firm risk (i.e. the annualized volatility of daily stock returns). Main results are supported with alternative measures of firm risk and estimation methods.FindingsThe study finds that increasing TMT gender diversity leads to a reduction in firm risk-taking in the hospitality industry. Moreover, the study finds that hospitality firms led by a female CEO experience lower firm risk compared to firms led by a male CEO. Finally, the study finds evidence that the relationship between TMT gender diversity and firm risk is contingent on the level of incentive pay awarded to TMT members and the age of TMT members. Increasing incentive pay and aging executive teams decrease the risk reduction effect of TMT gender diversity.Practical implicationsThe findings of this study recommend that firm risk-taking in the hospitality industry is related to gender diversity in TMTs. Hence, the board of directors should pay attention to gender composition for executive positions for risk management. Moreover, the results also suggest that care should be exercised when using incentive pay to align the interests of managers and shareholders. Finally, the board of directors needs to consider both gender diversity and age of the TMT members for TMT composition to manage executives’ risk-taking behavior.Originality/valueThis study fills a research gap in the hospitality literature by providing empirical evidence for the link between TMT gender diversity and firm risk-taking. Additionally, the study introduces incentive pay and age of TMT as contingency factors for the link between TMT gender diversity and firm risk-taking.
Rethinking the wine list: restaurant customers’ preference for listing wines according to wine style
Authors: Staub Cornelia , Siegrist Michael 
Published: February 2022

Purpose
The purpose of this research paper was to examine difficulties that consumers have when choosing wine in a restaurant and whether structuring a wine list according to taste rather than origin facilitates decision-making.

Design/methodology/approach
The authors conducted two online studies in Switzerland to assess whether a wine list organized according to wine style categories, such as fresh and dry or bold and fruity, reduces consumers’ perceived difficulty of choice compared to a traditional origin-grouped wine list and which wine list consumers prefer. In the first study (N = 577), participants received either an origin- or a style-grouped wine list. In the second study (N = 276), participants received and evaluated both wine lists.

Findings
In Study 1, participants with the style-grouped wine list had a longer decision time, but perceived difficulty of choosing a wine was the same as that for the origin-grouped wine list. Study 2 revealed that participants strongly preferred either the origin- or style-grouped wine list. Overall, more than half the participants preferred the style-grouped wine list, indicating that many consumers may appreciate wine lists organized according to wine style.

Practical implications
The findings underscore that restaurants should recognize customer preferences for wine list structures, which may influence customer satisfaction.

Originality/value
This paper assesses consumer perceptions of different wine list structures and how these affect consumers’ selection of a wine in a restaurant.
A humanoid robotic hand capable of internal assembly and measurement in spacesuit gloves
Authors: Bai Rui , Kang Rongjie , Shang Kun , Yang Chenghao , Tang Zhao , Wang Ruiqin , Dai Jian S. 
Published: February 2022

Purpose
To identify the dexterity of spacesuit gloves, they need to undergo bending tests in the development process. The ideal way is to place a humanoid robotic hand into the spacesuit glove, mimicking the motions of a human hand and measuring the bending angle/force of the spacesuit glove. However, traditional robotic hands are too large to enter the narrow inner space of the spacesuit glove and perform measurements. This paper aims to design a humanoid robot hand that can wear spacesuit gloves and perform measurements.

Design/methodology/approach
The proposed humanoid robotic hand is composed of five modular fingers and a parallel wrist driven by electrical linear motors. The fingers and wrist can be delivered into the spacesuit glove separately and then assembled inside. A mathematical model of the robotic hand is formulated by using the geometric constraints and principle of virtual work to analyze the kinematics and statics of the robotic hand. This model allows for estimating the bending angle and output force/torque of the robotic hand through the displacement and force of the linear motors.

Findings
A prototype of the robotic hand, as well as its testing benches, was constructed to validate the presented methods. The experimental results show that the whole robotic hand can be transported to and assembled in a spacesuit glove to measure the motion characteristics of the glove.

Originality/value
The proposed humanoid robotic hand provides a new method for wearing and measuring the spacesuit glove. It can also be used to other gloves for special protective suits that have highly restricted internal space.
Modelling the strategies for improving maturity and resilience in medical oxygen supply chain through digital technologies
Authors: K.E.K. Vimal , Nadeem Simon Peter , Meledathu Sunil Siddharth , Suresh Gokul , Sanjeev Navaneeth , Kandasamy Jayakrishna 
Published: February 2022

Purpose
Improving the medical oxygen supply chain (MOSC) is important to cope with the uneven demand and supply seen in the MOSC when India faced the second wave of COVID-19. This improvisation increases the supply chain (SC) maturity and consequently the efficiency and resiliency to tackle oxygen shortage across the country and to prevent another similar scenario from ever happening. The purpose of this study is to identify and prioritize the solutions to overcome the issues faced by the MOSC during the second wave of COVID-19 cases in India and in turn reduce the extent of casualties in the expected third wave.

Design/methodology/approach
This paper uses best worst method (BWM) and fuzzy technique for order performance by similarity to ideal solution to classify the sub-criteria for solutions to solve major SC issues. BWM is used to determine the weights of the sub-criteria and fuzzy technique for order performance by similarity to ideal solution for the final ranking of the solutions to be adopted.

Findings
The result of this study shows that the Internet of Things based tagging system is the best solution followed by horizontal and vertical integration of SC in making a resilient and digitized MOSC capable of handling general bottlenecks during a possible third wave.

Research limitations/implications
The research provides insights that can enable the personnel involved in MOSC. Proper understanding will help the practitioners involved in the SC to effectively tailor the operations and to allocate the resources available in an effective and dynamic manner by minimizing or eliminating the pre-existing bottlenecks within the SC.

Originality/value
The proposed framework provides an accurate ranking and decision-making tool for the implementation of the solutions for the maturity of the MOSC.
Research strength index to identify the performance of research universities: the case of Indonesia
Authors: Dimyati Muhammad , Putera Prakoso Bhairawa , Laksani Chichi Shintia , Zulhamdani Muhammad , Handoyo Setiowiji , Rianto Yan , Handoko Laksana Tri 
Published: February 2022

Purpose
This paper aims to identify the strengths of the universities based on the research area which are prioritized by the Government of Indonesia in the National Medium Term Development Plan 2015–2019, in the research areas of food and agriculture; energy, new and renewable energy; health and medicine; transportation; telecommunication, information and communication; defense and security technologies and advanced materials.

Design/methodology/approach
The mapping of the research strength in Indonesian universities is performed by using data of the university research output in the Information System for Research and Community Service, Ministry of Research, Technology and Higher Education and categorized into seven categories, i.e. accredited national journals, international journals indexed by Scopus or others, Intellectual Property ownership, textbooks, prototypes and an appropriate technology. Based on the data obtained from Information System for Research and Community Service, there are 904 universities in Indonesia conducting research activities and generating 14,188 research outputs.

Findings
This paper analyzes 3 of the 9 National Research Priorities, namely, food, energy and health and medicine. The data show that there are 904 universities in Indonesia conducting research activities and producing 14,188 research results. The research strength index based on National Research Priorities shows that three universities have the highest cluster strength index. Gadjah Mada with an index value of 4.95 is the highest index in the food cluster. In the energy cluster of the Institut Teknologi Bandung with the highest index value of 0.63. Meanwhile, the Universitas Indonesia reached 2.06 as the highest index value in the health and medicine cluster.

Originality/value
Measurement of the strength of the study was conducted using data from research results from universities in 2016 which were recorded in the Information System for Research and Community Service. The University’s R&D strength is calculated from seven categories of research results: accredited national journals, international journals indexed by Scopus or others, intellectual property rights, textbooks, prototypes and appropriate technology products.
Development of institutional repositories in academic and research libraries in Nigeria
Authors: Eromosele George Osas , Adesina Olabisi Fadeke , Abdulrazaq Mutalib Olanrewaju , Aliyu Mahmud 
Published: February 2022

Purpose
The development of an institutional repository for academic and research libraries makes possible the visibility of localized scholarly contents on web platform and also provide open access to restricted resources. This paper aims to explore the relevance of developing institutional repositories for open and unrestricted access to confined resources, and discusses academic and research libraries roles in developing institutional repositories; the types of software to be used for the development; and the hardware requirements for server setup and copyright issues. With more establishment of institutional repositories, access to localized information domiciled in the remote institutions can be easily accessed by visiting the institutional library online to retrieve the material.

Design/methodology/approach
To provide a thorough breakdown of the building of institutional repositories in the University of Ilorin, Library, webliography sources were consulted.

Findings
It has been established by the OpenDOAR which is the quality-assured, global Directory of Open Access Repositories that only 30 repositories have been created by some academic and research libraries in Nigeria. There is a need for more academic and research libraries in Nigeria to key into the initiative of developing online institutional repositories to give online visibility to their intellectual contents that have no copyright restrictions in meeting the researcher’s information needs.

Originality/value
This study revealed a thorough approach and various steps that should be followed in developing institutional repositories for academic and research libraries.