bluuebunny/crossref_metadata_2025_split huggingface dataset:
Facile production of binary polymer/carbonic nanofiller‐based biodegradable electromagnetic interference shield films with low electrical percolation threshold
Authors: Nath Krishnendu , Ghosh Suman Kumar , Katheria Ankur , Das Palash , Das Narayan Chandra 
Published: September 2022

AbstractInterference of electromagnetic (EM) radiation generated from different electronic gadgets produces noises diminishing their performance. So, shielding of EM interferences (EMI) is necessary to achieve noise‐free performance from electronic gadgets. In the present work, we have produced thermoplastic polyurethane (TPU)/polybutylene adipate‐co‐terephthalate (PBAT) based biodegradable binary polymeric blend and incorporated different carbonic nanofillers such as Vulcan XC‐72 conductive carbon black (VCB) and multiwalled carbon nanotube (MWCNT) into it employing solvent mixing and casting technique and studied the morphology of polymer blends, EMI shielding effectiveness in X‐band of the EM spectrum as well as mechanical performance, electrical performance, and biodegradability of polymer nanocomposites. The selective nanofiller distribution in the low viscous PBAT phase of the “co‐continuous” binary blend is found to achieve a “lower electrical percolation threshold” compared to neat polymer nanocomposites. For each of the TPU/PBAT/VCB and TPU/PBAT/MWCNT nanocomposites, electrical percolation threshold of around 12.5 and 5 wt% respectively has been achieved successfully. Nanocomposite sheets with a thickness of 1.0 mm and loading of 30 wt% of VCB and 15 wt% of MWCNT gave EMI shielding values of −25 dB and −28 dB respectively at a frequency of 8.4 GHz.
Smart polydimethylsiloxane sponges with high piezoelectric responses
Authors: Hu Hang 
Published: September 2022

AbstractIn recent years, soft and stretchable electrets have drawn considerable attention for their wide applications in energy harvesters, sensors, and actuators. However, existing electrets struggle to attain both longevity and stretchability (softness), which means charge retention capability and stretchability (softness) of electrets conflict. In this article, one novel soft and stretchable electret (PPSE) with great electromechanical performance is demonstrated. The PPSE is composed of a polydimethylsiloxane (PDMS) sponge and polytetrafluoroethylene (PTFE) particles. The uniformly distributed pores obtained by the sugar leaching method inside the PPSE can not only accumulate macroscopic dipoles but also provide great stretchability, flexibility, and softness for the material. The resulting PPSE has a large value up to 477 pC/N and great charge stability. Moreover, by the virtue of stretchability and softness, it can output electric signals from twisting, bending, and other mechanical interactions without breaking apart. With these great performances, PPSE will potentially enable applications for actuation, sensing, and energy harvesting.
Indirubin regulates T cell differentiation by promoting αVβ8 expression in bone marrow‐derived dendritic cells to alleviate inflammatory bowel disease
Authors: Zhang Tao , Peng Hong , Li Yunxiang , Zhou Xiaoqing , Pu Wenfeng , Zhang Yan , Du Zhonghan , Wei Fuxia , Li Siqing , Zhou Qian 
Published: September 2022

AbstractInflammatory bowel disease is a disease that can invade the whole digestive tract and is accompanied by immune abnormalities. Immune dysfunction involving dendritic cells (DCs) and T cells is recognized as a key factor in diseases. Indirubin (IDRB) exerts antiinflammatory effects and can help in treating immune diseases. This study aimed to isolate bone marrow‐derived dendritic cells (BMDCs) using lipopolysaccharide (LPS) to obtain mature DCs (mDCs). The expression of CD80, CD86, CD40, and MHC‐II was detected using flow cytometry after treatment with IDRB. αVβ8 siRNA was used to knock down αVβ8 in mDCs, and the expression of CD80, CD86, CD40, and MHC‐II was detected. Meanwhile, DCs were co‐cultured with T cells. Then, T cell differentiation was detected using flow cytometry, and the cytokine levels were detected using enzyme‐linked immunosorbent assay. The animal model of dextran sulfate sodium (DSS)‐induced inflammatory bowel disease was established in mice. After intervention with IDRB and αVβ8 shRNA, the intestinal tissues were evaluated using H&E staining, disease activity index (DAI) score, and histological damage index, and the corresponding factors and cytokines to regulatory T cells (Treg) and Th17 were measured. The results showed that αVβ8 was expressed in immature DCs and mDCs. CD80, CD86, CD40, and MHC‐II expression decreased after IDRB treatment in mDCs. Meanwhile, the expression of TNF‐α and TGF‐β also decreased after IDRB treatment. The effect of IDRB on the expression of CD80, CD86, CD40, MHC‐II, TNF‐α, and TGF‐β in mDCs was reversed by αVβ8 siRNA. The Treg differentiation increased after IDRB treatment, while the differentiation of Th17 cells was inhibited. This effect of IDRB was reversed by mDCs after treatment with αVβ8 siRNA. In vivo experiments showed that IDRB alleviated the symptoms of inflammatory bowel disease in animals. Enteritis significantly reduced, and the effect of IDRB was reversed by αVβ8 shRNA. The results suggested that IDRB regulated the differentiation of T cells by mediating the maturation of BMDCs through αVβ8. This study confirmed the therapeutic effect of IDRB in inflammatory bowel disease and suggested that IDRB might serve as a potential drug.
Antibacterial activity and synergistic antibiotic mechanism of trialdehyde phloroglucinol against methicillin‐resistant Staphylococcus aureus
Authors: Shen Dingyi , Duan Songpo , Hu Yuhan , Liang Jintong , Tang Youzhi 
Published: September 2022

AbstractThe emergence of methicillin‐resistant Staphylococcus aureus (MRSA) has become a critical global concern. Identifying new anti‐S. aureus agents or therapeutic strategies are urgently needed to treat S. aureus infection. The present study investigated the antibacterial activity of 16 phenolic compounds against MRSA, four of which exhibited antibacterial activity. Their antibacterial activities increased in a dose‐dependent manner but showed different responses with the extension of treatment time. Trialdehyde phloroglucinol (TPG) and 2‐nitrophloroglucinol (NPG) maintained stable antibacterial activity; however, that of dichlorophenol and myricetin decreased rapidly over 24 hr of treatment. Checkerboard and time‐kill assays indicated that TPG and NPG exhibited strong synergistic antibacterial activities with penicillin or bacitracin. Microscopic observation and membrane integrity analysis showed that the combination of TPG and penicillin destroyed the MRSA cell membrane, resulting in the leakage of intracellular biomacromolecules, marked changes in surface zeta potential, and the collapse of membrane potential. Moreover, the combination significantly decreased penicillinase activity and penicillin‐binding protein 2a mRNA expression, inhibiting MRSA growth. Taken together, these results demonstrated that the combination of the phloroglucinol derivative TPG and penicillin has significant synergistic anti‐MRSA activity and can serve as a potential therapeutic strategy to treat MRSA infections.
DNAzyme‐Assisted Nano‐Herb Delivery System for Multiple Tumor Immune Activation
Authors: Du Shiyu , Chen Chao , Qu Suchen , Song Hongxiu , Yang Jingjing , Li Yayao , Liu Kunguo , Lu Qianglan , Luo Wen , Wang Runtian , Guan Xiaoxiang , Song Yujun , Han Xin 
Published: September 2022

AbstractAs a promising therapeutic strategy against cancer, immunotherapy faces critical challenges, especially in solid tumors. Immune checkpoint blockade therapy, particularly blocking the interaction of the programmed cell death 1 (PD1)‐PD1 ligand 1 (PD‐L1) axis, can reverse the suppression of T cells so as to destroy tumor cells and exert antitumor effects. Here, a strategy of multiple activation of immune pathways is developed, to provide supporting evidence for potential antitumor therapies. Briefly, a pH/glutathione responsive drug‐loading hollow‐manganese dioxide (H‐MnO2)‐based chlorine6 (Ce6)‐modified DNAzyme therapeutic nanosystem for the combination of gene therapy and immunotherapy is established. The H‐MnO2 nanoparticles could efficiently deliver the DNAzyme and glycyrrhizic acid (GA) to enhance the tumor target effects. In the tumor microenvironments, the biodegradation of H‐MnO2 via pH‐induced hydrolyzation allows the release of guest DNAzyme payloads and host Mn2+ ions, which serve as PD‐L1 mRNA‐targeting reagent and require DNAzyme cofactors for activating gene therapy. In addition, Mn2+ is also associated with the immune activation of thcGAS‐STING pathway. Auxiliary photosensitizers Ce6 and GA could produce reactive oxygen species, resulting in immunogenic cell death. Overall, this study provides a general strategy for targeted gene inhibition and GA release, which is valuable for the development of potential tumor immunotherapies.
Chameleon‐Like Fluorescent Probe for Monitoring Interplays between Three Organelles and Reporting Cell Damage Processes through Dramatic Color Change
Authors: Zhang Zheming , Yang Shuxian , Dong Baoli , Kong Xiuqi , Tian Minggang 
Published: September 2022

AbstractThe in‐depth study of the interplay and cooperation between multiple organelles is an important biological task. Single fluorescent probes for separate visualization of multiple organelles is a desirable molecular tool, but the construction of such a probe is extremely difficult owing to the lack of valid strategies. In this work, utilizing the reversible cyclization reaction and intermolecular π stacking mechanism, a robust fluorescent probe is constructed to discriminatively illuminate lipid droplets (LDs), mitochondria, and lysosomes with blue, green, and red emission colors, respectively. Using the probe, the interplays and cooperation between LDs, mitochondria, and lysosomes are successfully studied, and the critical roles of lysosomes and LDs during mitochondrial fission are successfully revealed. Furthermore, this unique probe reveals the sequential damage of mitochondria and lysosomes during apoptosis through the successive fading of green and red emission. Thereby, the probe enables the discrimination of health state, early apoptosis, and late apoptosis of cells with three different sets of fluorescent signals. Overall, the robust probe is a desirable molecular tool to reveal the interactions between the three organelles, and investigate cell apoptosis and relative areas.
Thiophene‐2,3‐Dialdehyde Enables Chemoselective Cyclization on Unprotected Peptides, Proteins, and Phage Displayed Peptides
Authors: Wei Tongyao , Li Dongfang , Zhang Yue , Tang Yubo , Zhou Haiyan , Liu Han , Li Xuechen 
Published: September 2022

AbstractOrtho‐phthalaldehyde has recently found wide potentials for protein bioconjugation and peptide cyclization. Herein, the second‐generation dialdehyde‐based peptide cyclization method is reported. The thiophene‐2,3‐dialdehyde (TDA) reacts specifically with the primary amine (from Lys side chain or peptide N‐terminus) and thiol (from Cys side chain) within unprotected peptides to generate a highly stable thieno[2,3‐c]pyrrole‐bridged cyclic structure, while it does not react with primary amine alone. This reaction is carried out in the aqueous buffer and features tolerance of diverse functionalities, rapid and clean transformation, and operational simplicity. The features allow TDA to be used for protein stapling and phage displayed peptide cyclization.
Taxonomic and palaeobiological implications of a large, pathological sabretooth (Carnivora, Felidae, Machairodontinae) from the Lower Pliocene of South Africa
Authors: Rabe Caitlin , Chinsamy Anusuya , Valenciano Alberto 
Published: September 2022

AbstractWe describe the most complete postcranial remains of a pathological, large‐bodied sabretooth from the Lower Pliocene site of Langebaanweg ‘E’ Quarry (South Africa). The skeleton consists of hind limb and vertebral elements that exhibit distinctive exostoses, osteophytes and eburnation. We performed a quantitative morphological comparison of the new postcranial remains found in Langebaanweg, with other Neogene and Quaternary sabretooth and non‐sabretooth felids, consisting of the genera Amphimachairodus, Machairodus, Lokotunjailurus, Dinofelis, Panthera, Homotherium and Smilodon from several sites in Africa, Europe and North America, using principal component analysis and Mosimann transformations. Although the pathological deformation of the remains distorted some of the linear measurements, most of the analysed variables do not contain pathological features, and strongly indicate that the Langebaanweg sabretooth is morphologically closer to Machairodus aphanistus and Lokotunjailurus emageritus than it is to Amphimachairodus giganteus. This indicates that the remains could belong to an undetermined sabretooth species from the Langebaanweg locality. The observed pathologies in the foot and lumbar spine are consistent with diagnostic criteria for severe osteoarthritis (due to maturity), which would have limited limb mobility with severe consequences for hunting success.
Vision Zero in Disease Eradication
Authors: Rosenberg Mark , Laney Emaline , Tingvall Claes 
Published: September 2022

AbstractThe eradication of smallpox – the first and so far only human disease to be eradicated – generated a tremendous amount of excitement and attention to the idea of disease eradication and elimination. In the twentieth century alone, smallpox had claimed the lives of more than 300,000,000 people (Sneed A, Scientific American, https://www.scientificamerican.com/article/who-remember-smallpox/, 2014). The last natural case occurred in 1977, almost 200 years after the smallpox vaccine was discovered, and in 1979 the World Health Organization declared that the disease had been eradicated (WHO, Smallpox, World Health Organization. World Health Organization. https://www.who.int/csr/disease/smallpox/en/, 2019c). Most importantly, it demonstrated that eradication was possible; that through the use of science, we could change our future for the better in ways that we had not even dared to think about previously. This achievement also helped to focus attention on clear definitions of disease eradication and elimination. Since then, these definitions have been continuously evolving. In 1993, the International Task Force for Disease Eradication (ITFDE I) defined disease control, elimination, and eradication. With that, they evaluated over 90 potential infectious disease candidates and concluded that six were eradicable. Subsequently in 1997, the Dahlem Workshop focused on the science of eradication and defined a range of public health approaches to infectious diseases from control, to elimination of disease, elimination of infections, eradication, and extinction. Three years later, the second International Task Force for Disease Eradication (ITFDE II) convened in 2000 to review the progress over the past decade.Inspired by these efforts, we reviewed our own experience and interviewed leaders of several disease eradication and elimination campaigns to identify lessons learned from a variety of disease elimination campaigns including smallpox, polio, Guinea worm, and onchocerciasis. Our aim was to identify lessons that might be applied to road traffic injuries. We identified 12 lessons from these infectious disease campaigns: (1) this is a cause and effect world; (2) know the truth; (3) coalitions are absolutely essential and absolutely hard; (4) avoid certainty (the Achilles heel of science); (5) measure frequently and build in continuous improvement; (6) respect the culture and work with the people you are trying to help; (7) the best decisions are based on the best science, but the best results, on the best management; (8) the best solutions move us closer to global health equity; (9) do not underestimate the time, resources, or tenacity it will take succeed; (10) eradication does not always require a vaccine or a cure; (11) start in the most difficult places first; and (12) you don’t begin at the end. These concepts from infectious disease campaigns have broader public health applications, and we discuss some of the implications for reducing road traffic injuries.
Miscommunications Based on Different Meanings of “Safe” and Their Implications for the Meaning of Safe System
Authors: Sakashita Chika , Job R. F. Soames , Belin Matts-Åke 
Published: September 2022

AbstractWhile many countries are apparently adopting “Safe System” for road safety, the failure to deliver the vision of zero deaths and serious injuries continues in part due to the lack of a rigorous and agreed definition of “safe” in road safety. Multiple authoritative definitions of the adjective “safe” exist which may be categorized as probabilistic and absolute. While apparently similar, these definitions are in a fundamental sense inconsistent with each other. The probabilistic definition involves degrees of safety, through probabilities that harm is not likely or unlikely, or that there is little risk. The absolute definition presents safety as free from harm or not involving any risk or protected from danger. Road safety is currently communicated as though there is an agreed meaning of safe, but the vital conversation around what is meant by safe is not undertaken because the difference in usage of the term safe is not appreciated. For example, in road design and engineering, road design standards are generally developed to achieve this probabilistic definition of safety and not absolute safety: the road can be described as safe because it by itself (with perfect use) will not cause a crash, even though people still die on it. Based on the absolute definition of safe, such roads are not safe as unambiguously demonstrated by people dying on them. Calls for roads to be made safe employing the absolute definition are often met with the response that they are already safe (in the probabilistic sense), having been built to “accepted” design guidelines. The acceptance of the probabilistic definition of safe for Safe System hinders progress toward its fundamental aims of zero deaths and serious injuries. In order to achieve zero deaths and serious injuries, uniform understanding and acceptance of “safe” adopting the absolute definition is needed.
Collateral Capacity Assessment
Authors: Yang Wenjin , Soomro Jazba , Jansen Ivo G. H. , Venkatesh Aashish , Yoo Albert J. , Lopes Demetrius , Beenen Ludo F. M. , Emmer Bart J. , Majoie Charles B. L. M. , Marquering Henk A. 
Published: September 2022

Abstract
Background and Purpose
Intracranial collateral capacity is conducive to imply parenchymal perfusion of affected territory after acute vessel occlusion. The Tan collateral score is commonly used to assess the intracranial collateral capacity; however, this score is coarsely grained and interobserver agreement is low, which reduces prognostic value and clinical utility. We introduce and evaluate an alternative extended Tan score based on the conventional Tan scale and assess the agreement with a quantitative score.

Methods
We included 100 consecutive patients with a proven acute single large vessel occlusion of the proximal anterior circulation. Collaterals were graded with the conventional and extended Tan score and an automated quantitative score. The extended Tan score is a finer 6‑scale manual score based on the conventional 4‑point Tan scale. The quantitative score is calculated by an automatic software package (StrokeViewer). Interobserver agreement of the manual scores was assessed with the weighted kappa. The Spearman correlation coefficient was calculated to determine the agreement between the manual and automated collateral scores.

Results
The interobserver agreement was higher for the extended score than for the conventional score with a weighted kappa of 0.70 and 0.65, respectively. For the extended and conventional score, the Spearman correlation coefficient for the agreement with the automated score was 0.78 and 0.76, respectively.

Conclusion
Because of the good interobserver agreement and good agreement with quantitative assessment, the extended collateral score is a strong candidate to improve prognostic value of collateral assessment and implementation in clinical practice.
Investigation and prediction of central cracking in cross wedge rolling
Authors: Zhou Xianyan , Sun Chaoyang , Wang Baoyu , Jiang Jun 
Published: September 2022

AbstractCentral cracking refers to the formation of internal cavities in cross wedge rolling (CWR) products. It occurs in various materials such as aluminium/titanium alloys, steels and plasticine at room or elevated temperatures, driven by different central cracking mechanisms. However, these mechanisms are still elusive, and a unified central cracking predictive model is absent due to the complex stress states within the workpiece, including triaxial stress states, cyclic loading and severe shear effects. In this study, the underlying fracture mechanisms were revealed, and a robust unified damage model with sound physical meanings was developed using a lab-scale CWR physical model and finite element models. The physical model with the plasticine billets was built, allowing the CWR dies with different geometries rapidly 3D printed and the billets with various ductility efficiently manufactured. The central cracking transiting from brittle to ductile fracture was experimentally observed for the first time using specifically designed plasticine/flour composite samples at varying ductility. The corresponding physics-based central cracking predictive model was proposed and validated quantitatively with 60 groups of CWR tests and compared with ten existing damage models/fracture criteria. This study effectively solves the long-lasting central cracking problem in the CWR industry and enhances the scientific understanding of fracture mechanics in complex engineering applications.
Radiogenomic markers enable risk stratification and inference of mutational pathway states in head and neck cancer
Authors: Spielvogel Clemens P. , Stoiber Stefan , Papp Laszlo , Krajnc Denis , Grahovac Marko , Gurnhofer Elisabeth , Trachtova Karolina , Bystry Vojtech , Leisser Asha , Jank Bernhard , Schnoell Julia , Kadletz Lorenz , Heiduschka Gregor , Beyer Thomas , Hacker Marcus , Kenner Lukas , Haug Alexander R. 
Published: September 2022

Abstract

Purpose
Head and neck squamous cell carcinomas (HNSCCs) are a molecularly, histologically, and clinically heterogeneous set of tumors originating from the mucosal epithelium of the oral cavity, pharynx, and larynx. This heterogeneous nature of HNSCC is one of the main contributing factors to the lack of prognostic markers for personalized treatment. The aim of this study was to develop and identify multi-omics markers capable of improved risk stratification in this highly heterogeneous patient population.

Methods
In this retrospective study, we approached this issue by establishing radiogenomics markers to identify high-risk individuals in a cohort of 127 HNSCC patients. Hybrid in vivo imaging and whole-exome sequencing were employed to identify quantitative imaging markers as well as genetic markers on pathway-level prognostic in HNSCC. We investigated the deductibility of the prognostic genetic markers using anatomical and metabolic imaging using positron emission tomography combined with computed tomography. Moreover, we used statistical and machine learning modeling to investigate whether a multi-omics approach can be used to derive prognostic markers for HNSCC.

Results
Radiogenomic analysis revealed a significant influence of genetic pathway alterations on imaging markers. A highly prognostic radiogenomic marker based on cellular senescence was identified. Furthermore, the radiogenomic biomarkers designed in this study vastly outperformed the prognostic value of markers derived from genetics and imaging alone.

Conclusion
Using the identified markers, a clinically meaningful stratification of patients is possible, guiding the identification of high-risk patients and potentially aiding in the development of effective targeted therapies.

Graphical abstract