bluuebunny/crossref_metadata_2025_split huggingface dataset:
Annonaceous acetogenins: A computational study into their antitumor activity in multidrug resistant enzymes
Authors: Alanzi AbdullAllah , Moussa Ashaimaa Y. 
Published: December 2022

Abstract
Natural products that could reverse tumor drug resistance are promising and highly appreciated; especially, if they are part of combination therapy with conventional anti-cancer drugs. Acetogenins, the long chain polyketide derived fatty acids, from Annona squamosa and muricata were screened in the last 20 years and promising compounds were selected to investigate their activity by computational studies inside the binding pockets of four major MDR enzymes (EGFR, MCL-1, BCL-2, and NQO1). Using virtual screening and molecular docking, compounds were ranked according to their fitting scores and squamostanin A, annupupuricin, Squamocin IV were the best fitting molecules with favorable non covalent interactions with key amino acids compared to native ligands and standards. Subsequently, these compounds in their prepared enzyme complexes were subjected to molecular dynamics stability study to investigate the complex characteristics. The key interactions were confirmed in the molecular dynamic study, which manifested no noticeable unfolding of the binding complexes. The physicochemical and drug likeness properties of all the compounds were evaluated through the QikProp module indicating their suitable ADMET properties within the recommended ranges.
A New, Alternative Risk Score for Sarcopenia in Chinese Patients with Type 2 Diabetes Mellitus
Authors: He Qinghua , Wang Xiuzhi , Yang Caizhe , Zhuang Xiaoming , Yue Yanfen , Jing Hongjiang , Hu Jing , Sun Mingxiao , Guo Lixin 
Published: December 2022

Abstract
Objective
To develop a new, alternative sarcopenia risk score to screen for sarcopenia in type 2 diabetes patients in China and to demonstrate its validity.
Research design and methods
: The data for this study came from a multicenter, cross-sectional study that had been designed to estimate the prevalence of sarcopenia among adults with type 2 diabetes and had been conducted in several hospitals in Beijing, China. A total of 1125 participants were randomly divided into two groups: an exploratory population and a validation population. A multivariable logistic regression model using the backward stepwise likelihood ratio method to estimate the probability of sarcopenia was fitted with candidate variables in the exploratory population. A new, alternative sarcopenia risk score was developed based on the multivariable model. The internal and external validations were performed in the exploratory and validation populations. The study was registered at Chinese Clinical Trial Registry (ChiCTR-EOC-15006901).
Results
The new, alternative sarcopenia risk score included seven variables: age, sex, BMI, glycosylated hemoglobin, insulin treatment or not, total caloric intake per day, and the proportion of calorie supplied by protein. The score ranged from − 9 to 23. The area under the receiver operating characteristic (ROC) curve of the risk score for the prediction of sarcopenia in type 2 diabetes patients was 0.812 (95% CI 0.748–0.877) and 0.841 (95% CI 0.785–0.897) in the exploratory and validation populations, respectively. At the optimal cutoff value of 11, the sensitivity and specificity of the score for the prediction of sarcopenia were 74.5% and 78.0% in the exploratory population and 80.5% and 72.2% in the validation population, respectively. The Hosmer-Lemeshow goodness-of-fit test showed a good calibration with the risk score in external validation (χ2 = 1.687, P = 0.989).
Conclusions
The new, alternative sarcopenia risk score appears to be an effective screening tool for the identification of sarcopenia in Chinese patients with type 2 diabetes in clinical practice.
Development Of A New Nanosensor For The Determination Of Food Coloring Sunset Yellow In Powder Drinks Using L-Cysteine Coated Copper Nanoclusters
Authors: Bilkay Mehmetcan , Karataş Büşra , Kara Hayriye Eda Satana 
Published: December 2022

Abstract

Sunset Yellow (SY) is an artificial colorant containing an azo group. The high stability and low cost of SY make it more preferable. Excessive consumption of SY, which is used to improve the appearance of foods, has harmful effects. The most important of these effects are cancer and hyperactivity disorder. Therefore, determination of SY in foods is extremely important for human health. In this method, L-cysteine ​​coated copper nanoclusters (CuNCs) were used as a fluorescence probe. L-cysteine ​​has been used as both a reducing and stabilizing agent. One-step green hydrothermal synthesis of CuNCs was made. L-cysteine-coated CuNCs have been characterized using several of methods. CuNCs quenching mechanism is static and inner filter effect (IFE). The linear range is 0.65-14 µg.ml− 1 at optimum conditions. LOD and LOQ values ​​were calculated as 0.1–0.35 µg.ml− 1, respectively. The proposed method was used for the determination of SY in different type of powder drinks. The developed nanosensor is environmentally friendly, easy, fast, reproducible, and low cost.
The role of TOP2A in immunotherapy and Vasculogenic mimicry in non-small cell lung cancer and its potential mechanism
Authors: wu Jiatao , Zhang Lei , Li wenjuan , Wang Luyao , Li Kairui , Shi Fan , Jia Qianhao , Liao Lingli , Shi Yuqi , Wu Shiwu 
Published: December 2022

Abstract
Background: Type IIA topoisomerase (TOP2A) is significantly associated with malignant tumor development, invasion, treatment and its prognosis, and has been shown to be a therapeutic target against cancer. In contrast, the role of TOP2A in the immunotherapy of non-small cell lung cancer as well as in VM formation and its potential mechanisms are unclear.
Methods: Based on the 82 significantly co-expressed genes of TOP2A screened, consensus molecular typing was performed by the NMF algorithm, and the effect of immunotherapy was further evaluated in two groups of patients with high and low risk. The expression of TOP2A and VM in non-small cell lung cancer tissues was assessed by immunohistochemistry. Western Blot, colony formation assay, CCK8 assay, cell cycle and apoptosis assay, tube-forming assay and cytoskeleton staining were used to verify the role of TOP2A in proliferation, skeleton regulation, motility and VM generation in non-small cell lung cancer and its mechanism.
Results: Patients with lung adenocarcinoma were distinguished into high- and low-risk subgroups based on significant co-expression of TOP2A genes. Subgroup analysis showed that patients in the low-risk group had a better prognosis, while higher risk was associated with higher tumor mutational load, M1-type macrophage and immune checkpoint molecule expression. The Tumor Immune Dysfunction and Rejection (TIDE) and Tumor Immunome Atlas (TCIA) databases also showed significant differences in the outcome of immunotherapy in patients with different types of lung adenocarcinoma. As verified by further clinical specimens, the presence of both TOP2A and VM were significantly and positively correlated with poor prognosis. TOP2A may ultimately affect immunotherapy and VM formation in non-small cell lung cancer through its involvement in regulating the expression of Wnt3a and PD-L1.
Conclusion: A model based on significantly co-expressed genes of TOP2A was significantly correlated with mutational load and immunotherapeutic effects in patients with non-small cell lung cancer. TOP2A plays an important role in immunotherapy and VM formation in non-small cell lung cancer through upregulation of Wnt3a and PD-L1 expression.
The switching process of trailing edge dominant tones on the different ladders
Authors: Song Zhangchen , Liu Peiqing , Guo Hao , Qu Qiulin , Hu Tianxiang 
Published: December 2022

Abstract
Airfoil trailing edge noise in low-to-moderate Reynolds number has multiple tonal noise. The frequency of the dominant tone has a ladder structure for the dependency on the free stream velocity, while the presence of the secondary tones is explained as an amplitude modulation by the primary single tone. However, during the jumping process, when the main tone is selected from two nearly equal strength tonal noise on the different ladders, the choice of the main tone and amplitude modulation theory are poorly investigated and lacks specific experiments at present. Hence, the switching process of trailing edge dominant tones of a NACA0012 airfoil at a Reynold number of around 130,000 is focused on by a simultaneous measurement of microphone and hot-wires at Beihang D7 aeroacoustics wind tunnel. The far field noise measurement confirms presence of two tones on time-average with a velocity range from 19.8m/s to 20.2m/s. For a freestream velocity of 20m/s, after selecting the time span by short-term Fourier transform, the wavelet contour maps of microphone present a random selection of the strongest tone, which is a switching tones pattern contradictory to the periodic amplitude modulation of only one tone. The simultaneous measurement results by two hotwires also show similar patterns with differences on the spanwise locations. This non-uniform spanwise alternation between frequencies rather than a consistent modification along the spanwise direction might contribute to the switching tones pattern in the microphone signals. A statistical analysis of the occurrence of two tones indicates that the switching tones pattern in sound pressure signals is greatly influenced by the spatial distribution of velocity on the spanwise direction as sound sources during the jumping process.
Do Hip and Ankle Strength as well as Range of Motion Predict Y-Balance Test - Lower Quarter Performance in Healthy Males?
Authors: Olszewski Maciej , Zając Bartosz , Mika Anna , Golec Joanna 
Published: December 2022

Abstract
The study aim was to evaluate associations between hip and ankle strength, ROM and YBT-LQ. The study involved 66 healthy males (age: 25.2+6.8 years). Each participant was assessed with ankle DF ROM, hip IR ROM, ER ROM and hip ABD, EXT and ER muscle isometric strength. The YBT-LQ test was performed for both limbs (stance and kicking leg). Forward two-step multiple linear regression analysis was performed to determine relationships between a predictor set and the criterion variable. Of 6 input variables for regression models, only 2 (ankle DF ROM, hip ABD strength) explained variance of YBT-LQ performance. Variance for anterior, posteromedial (PM) and posterolateral (PL) reach distances were explained from R2 = 0.15 to R2 = 0.49, but the models’ composition in PM and PL differed between legs. YBT-LQ composite score in both legs was explained by the same model, including ankle DF ROM and hip ABD (R2 = 0.44; R2 = 0.25). Hip ABD strength and ankle DF ROM assessment may be useful in individuals with poor YBT-LQ test performance to detect specific musculoskeletal deficits. Moreover, the regression models in stance leg for all reaches and composite score were more predictive than for the kicking leg.
An Availability Design to Capture Heterogeneous Decision Rules: a Case Study for Airfare Choice
Authors: Wang Jue , Smith Brett 
Published: December 2022

Abstract

In choice behaviour studies, decision makers are generally assumed to process all information for each available alternative and can choose by trading-off attributes at the margin. In many choice contexts, decision makers may employ alternative heuristics and we argue that this is likely to apply in airline itinerary choices. A population of choice makers employing multiple decision rules combined with heterogeneous preferences presents an added layer of complexity to the estimation of demand. This study presents an availability stated preference design aimed to differentiate decision rules used decision makers and, subsequently, provide more accurate demand responses. The empirical study reveals that air travellers apply different decision rules when choosing an airfare. Most adopt choice heuristics, such as non-trading (i.e. choosing the same carrier or fare class always) and lexicographic ordering on price. Identification of these rules leads to a better understanding of consumers’ airfare choice behaviour. Ignoring multiple decision rules leads to misguided yield management decisions.
WITHDRAWN: Study on multi-constraint and multi-objective optimization layout method of monitoring network for mine water inrush
Authors: Du Zhili , Wu Qiang , Zhao Yingwang , Zhang Xiaoyan , Yao Yi 
Published: December 2022

Abstract
Disastrous water inrush often causes heavy property losses and even casualties, while the current theory and technology of mine water prevention and control can not accurately predict the time and place of disastrous water inrush in mine, so monitoring disastrous water inrush has become an urgent problem to be solved. Through the networked deployment of water level sensors in the roadway, this paper proposes a monitoring network for catastrophic water inrush in coal mine, and studies a multi-constraint and multi-objective optimal deployment method for the monitoring network. By setting three practical constraints of mining area, risk level of water inrush and installation at specified location, and constructing two objective functions of minimum total cost and minimum average monitoring time, a mathematical model is established, and then NSGA - Ⅱ multi-objective genetic algorithm is designed to solve the model. As results, the method can optimize the monitoring network for mine water inrush from two dimensions of time and space. The proposed method is then verified in the Beiyangzhuang coal mine in the North China. The results show that the average time of the catastrophic water inrush monitored can be controlled within 916 seconds by using only 28 water level sensors, and the higher the risk level of water inrush, the shorter the monitoring response time. Due to the constraints of installation at specified location, the monitoring network can also take into account monitoring the daily water inflow in the Beiyangzhuang coal mine.
Diverse but Desolate Landscape of Gut Microbial Azoreductases: A Rationale for Idiopathic IBD Drug Response
Authors: Simpson Joshua B. , Sekela Josh J. , Carry Benjamin S. , Beaty Violet V. , Patel Shakshi , Redinbo Matthew R. 
Published: December 2022

Abstract
Background:
Prodrugs reliant on microbial activation are widely used but exhibit a range of efficacies that remain poorly understood. The anti-inflammatory compound 5-aminosalicylic acid (5-ASA), which is packaged in a variety of azo-linked prodrugs provided to most Ulcerative Colitis (UC) patients, show confounding inter-individual variabilities in response. Such prodrugs must be activated by azo-bond reduction to form 5-ASA, a process that has been attributed to both enzymatic and non-enzymatic catalysis. Gut microbial azoreductases (AzoRs) are the first catalysts shown to activate azo-linked drugs and to metabolize toxic azo-chemicals.
Results:
We chart the scope of the structural and functional diversity of AzoRs in health and in patients with the inflammatory bowel diseases (IBDs) UC and Crohn’s Disease (CD). Using structural metagenomics, we define the landscape of gut microbial AzoRs in 413 healthy donor and 1059 IBD patient fecal samples. Firmicutes encode a significantly higher number of unique AzoRs compared to other phyla. However, structural and biochemical analyses of distinct AzoRs from the human microbiome reveal significant differences between prevalent orthologs in the processing of toxic azo-dyes, and their generally poor activation of IBD prodrugs. Furthermore, while individuals with IBD show higher abundances of AzoR-encoding gut microbial taxa than healthy controls, the overall abundance of AzoR-encoding microbes is markedly low in both disease and health.
Conclusions:
Gut microbial AzoRs are functionally diverse but sparse in both health and disease, factors that may contribute to non-optimal processing of azo-linked prodrugs and idiopathic IBD drug responses.
Green Goals and Organic Produce: Towards the achievement of Sustainable Development Goals
Authors: Arora Manpreet 
Published: December 2022

Abstract
The current article is based upon bibliometric and secondary data where the author has contended to establish a connection of sustainable development and sustainable agricultural produce. The author believes that if organic practices in farming are adopted, they have the capacity to deal with multiple sustainable development goals including promoting the overall wellbeing of every living species. Present trends in the field of Organic Agriculture are seen to find out the themes where the acadecimicians are doing research.
Serum lipidomics reveals phosphatidylethanolamine and phosphatidylcholine disorders in patients with myocardial infarction and post-myocardial infarction-heart failure
Authors: Rong Jidong , He Tianmu , Zhang Jianyong , Bai Zhixun , Shi Bei 
Published: December 2022

Abstract
Background: Myocardial infarction (MI) and post-MI-heart failure (pMIHF) are major causes of death worldwide, however, the underlying mechanisms underlying pMIHF from MI are not well understood. The study sought to characterize early lipid biomarkers for the development of pMIHF disease.
Methods: Serum samples of 18 MI and 24 pMIHF patients were collected from the Affiliated Hospital of Zunyi Medical University and analyzed using lipidomics with Ultra High Performance Liquid Chromatography and Q-Exactive High Resolution Mass Spectrometer. All serum samples will be tested by the Official partial least squares discriminant analysis (OPLS-DA) to find the different expression of metabolites between the two groups. Furthermore, the metabolic biomarkers of pMIHF were screened using subject operating characteristic (ROC) curve and correlation analysis.
Results: The average age of 18 MI and 24 pMIHF participants is 57.83 ± 9.28 and 64.38 ± 10.89 years, respectively. The B-type natriuretic peptide (BNP) level was 328.5 ± 299.842 and 3535.96 ± 3025 pg/mL, TC was 5.59 ± 1.51 and 4.69 ± 1.13 mmol/L, and BUN was 5.24 ± 2.15 and 7.20 ± 3.49 mmol/L, respectively. In addition, 88 lipids, including 76 (86.36%) down-regulated lipids, were identified between the patients with MI and pMIHF. ROC analysis showed thatphosphatidylethanolamine (PE) (12:1e_22:0) (area under the curve [AUC] = 0.9306) and phosphatidylcholine (PC) (22:4_14:1) (AUC = 0.8380) were potential biomarkers. Correlation analysis showed that PE (12:1e_22:0) was inversely correlated with BNP and BUN, but positively correlated with TC. In contrast, the PC (22:4_14:1) was positively associated with both the BNP and the BUN, and was negatively associated with the TC.
Conclusions: Several lipid biomarkers were identified that could potentially be used to predict and diagnose patients with pMIHF. PE (12:1e_22:0) and PC (22:4_14:1) could sufficiently differentiate between patients with MI and pMIHF.
Full-thickness corneal suture analysis using anterior segment Ocular Coherence Tomography (AS-OCT)
Authors: Pérez Sara Margarita Pérez , Arias Valentina Cárdenas , Pino Margarita María Perilla , Martínez Camilo Eduardo 
Published: December 2022

Abstract
Purpose: To evaluate the anatomic and visual outcomes of patients who underwent full-thickness corneal suture in penetrating ocular trauma.
Methods: A retrospective observational study was conducted after analyzing the medical records of 7 patients who presented to the ohptalmology department of the Hospital Universitario San Ignacio, during January 2020 and December 2021, with diagnosis of penetrating ocular trauma requiring corneal suture with subsequent anterior segment ocular tomography (AS- OCT) at 2 weeks post-trauma.
Results: In 2 of the 7 patients included in the cohort intraocular foreign body was found. The average age at presentation of the trauma was 47 years, with a higher rate (71.4%) in men. The average time between trauma and surgery was approximately 2.6 days. In 100% of the patients the corneal architecture was restored, defined by the adequate apposition of the corneal endothelium between both sides of the wound.
Conclusions: The present study provides promising results regarding the use of full-thickness corneal suture in the context of penetrating ocular trauma. This intervention, employed at the 100% of the patients studied, adequately restores corneal architecture, positively impacting postoperative visual acuity.
Analysis of long term spatio- temporal trend of rainfall in Tamil Nadu between 1981-2020
Authors: Sembanan Kokilavani , Subbiah Ramanathan , Vellingiri Geethalakshmi , Ganesan Dheebakaran , Nakaranai Sathyamoorthy , Shanmugam Mohankumar 
Published: December 2022

Abstract
Increased anthropogenic activity in recent decades has resulted in major global climate change. This paper mainly focuses on the assessment of changes occurring in the spatio -temporal distribution of rainfall with 40-years database of monthly precipitation for Seasonal Precipitation Concentration Index (SPCI) and trends in Tamil Nadu. The hydro-meteorological time series rainfall data over a period of 40 years (1981–2020) was collected from Tamil Nadu Agricultural University and India Meteorological Department and subsequently analysed using various statistical methods for Tamil Nadu. The SPCI was analysed for both southwest and northeast monsoon. SPCI values (< 10) revealed that the rainfall was uniformly distributed in southwest and SPCI values (> 10) showed that more weather extremes were observed during northeast monsoon. Mann–Kendall, non-parametric test was done using trend software for both the monsoon. During southwest, significant increasing trend in rainfall was observed at Coimbatore (1.8mm/season/year), Erode (2.1mm/season/year), Perambular (2.1mm/season/year), Theni(2.0mm/season/year) and Tirunelveli (2.4 mm/season/year) while significant decreasing trend in rainfall was observed at Namakkal(2.5 mm/season/year). During northeast, significant increasing trend in rainfall was observed Kancheepuram (2.4 mm/season/year), Tutucorin(2.6 mm/season/year) and Villupuram(2.0 mm/season/year).
Association of HLA-DPA1 and DQA1 genes with PLE in Han and Tibetan males in Qinghai, China
Authors: Cao FaLong , Li JunPeng , Duan ZhiLi , Luo JunMing , Liu Xia , Zhang XiaoCen , Liu XueShan , Peng Yan 
Published: December 2022

Abstract
BACKGROUND
Polymorphic light eruption (PLE) is the commonest photosensitive disorder, characterized by pruritus, erythematous papules, plaques or blister lesions that occur within hours of exposure to ultraviolet radiation. However, the etiology and pathogenesis of PLE are still uncertain. The current study aims to identify the relationship between HLA and PLE, and analyze the discrepancies between Han and Tibetan males on the association of HLA with PLE.
METHODS
Polymerase chain reaction sequence-based typing (PCR-SBT) method was used to determine the distribution of HLA-DPA1 and -DQA1 alleles among 62 male patients with PLE (45 Hans and 17 Tibetans) and 66 healthy males (52 Hans and 14 Tibetans) in Qinghai region.
RESULTS
4 HLA-DPA1 and 11 HLA-DQA1 genotypes were detected in all samples. The allele frequency of HLA-DPA1*01:03 in healthy Han men was significantly higher than that in healthy Tibetan men (Pc=0.008), while the HLA-DPA1*02:01 was significantly lower than that in Tibetan men (Pc=0.04). The frequency of HLA-DQA1*03:03 allele (OR=2.7891, Pc=0.011) was significantly increased in Han men with PLE compared with Han controls , and HLA-DPA1*01:03 allele (OR=7.217, Pc=0.0076) was significantly increased in Tibetan men with PLE than Tibetan controls, whereas HLA-DPA1*02:01 (OR=0.154, Pc=0.008) allele frequency was highly decreased in Tibetan men with PLE.
CONCLUSIONS
There were racial differences between Han and Tibetan men in HLA gene polymorphism. The HLA-DQA1*03:03 allele could be a susceptible allele of PLE in Han men, and the HLA-DPA1*01:03 allele could be a susceptible allele of PLE in Tibetan men, while the HLA-DPA1*02:01 allele could be a protective allele in Tibetan men.