bluuebunny/crossref_metadata_2025_split huggingface dataset:
NAP1L5 targeting combined with MYH9 Inhibit HCC progression through PI3K/AKT/mTOR signaling pathway
Authors: Zhao Rui , Ge Yuzhen , Gong Yongjun , Li Bo , Xiao Benli , Zuo Shi 
Published: July 2022

Abstract
Purpose:Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death worldwide. Nucleosome assembly protein 1-like 5 (NAP1L5) is a protein-coding gene that encodes a protein similar to nucleosome assembly protein 1 (NAP1). It is a histone chaperone that plays an important role in gene transcription in organisms. However, the role of NAP1L5 in the pathogenesis of hepatocellular carcinoma remains to be elucidated.Method：We used QRT-PCR, immunohistochemistry and westernbolting to detect the expression of NAP1L5 in tissues and cell lines, and analyzed the relationship between its expression and clinicopathological features and prognosis. after up-regulating and down-regulating NAP1L5 in cell lines, we used a variety of methods to verify its regulatory effect on HCC, and verified it again in animal experiments, mass spectrometry analysis to find the possible binding protein (MYH9) and downstream signaling pathway (PI3K/AK/mTOR). And verify the relationship between them.Results: In this study, low expression of NAP1L5 was found in hepatocellular carcinoma, and the downregulation of NAP1L5 was related to shorter survival and disease-free survival. In addition, its expression is also related to the tumor size and recurrence of hepatocellular carcinoma. The overexpression and knockdown of NAP1L5 by plasmid and siRNA showed that NAP1L5 inhibited the proliferation, migration and invasion and induced apoptosis of hepatoma cells. In vivo experiments confirmed that NAP1L5 can inhibit the growth and metastasis of hepatocellular carcinoma cells. In the mechanistic study, we found that NAP1L5 affects the occurrence and development of hepatocellular carcinoma by regulating MYH9 to inhibit the PI3K/AKT/mTOR signaling pathway. Conclusion: As a functional tumor suppressor, NAP1L5 is expressed at low levels in HCC. NAP1L5 inhibits the PI3K/AKT/mTOR signaling pathway in hepatocellular carcinoma by regulating MYH9.It may be a new potential target for liver cancer treatment.
Anti-inflammatory and anti-oxidant activities of Gymnema sylvestre extract rescues acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway
Authors: JANGAM ARUNA , TIRUNAVALLI SATYA KRISHNA , ADIMOOLAM BALA MANIKANTHA , KASIREDDY BHAVANA , PATNAIK SAMATA SAI , Erukkambattu Jayashankar , Thota Jagadeshwar Reddy , Andugulapati Sai Balaji , ADDLAGATTA ANTHONY 
Published: July 2022

Abstract
Acute respiratory distress syndrome (ARDS) is one of the major cause of mortality in COVID-19 patients, due to limited therapeutic options. This prompted us to explore natural sources to mitigate this condition. Gymnema sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects. In this investigation, Gymnema sylvestre hydroalcoholic extract (HAEGS) was examined against lipopolysaccharide (LPS) induced lung injury and ARDS in in vitro and in vivo models. UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids. Gene expression by RTqPCR and DCFDA analysis by flow-cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive-oxygen-species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment. Consistent with the in vitro studies, we found that in LPS induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose dependent manner. Further mechanistic studies suggested that the HAEGS ameliorates the ARDS through the NF-κB/MAPK signaling pathway. Additional fractionation results revealed that fraction-6 which has the exclusive composition of gymnemic acid derivatives showed anti-inflammatory effects which is similar to the HAEGS. Overall, HAEGS significantly mitigated the LPS-induced lung injury and ARDS by targeting NF-κB/MAPK signaling pathway. Thus, our work unravels the protective role of HAEGS for the first time in managing ARDS.
Change in quality of life and psychological distress in patients with resected non-metastatic vs unresectable advanced cancer undergoing systemic treatment
Authors: Rodríguez-Gonzalez Adán , Carmona-Bayonas Alberto , Hernandez Raquel , Cruz-Castellanos Patricia , Velasco-Durantez Verónica , Antoñanzas-Basa Mónica , Lorente-Estelles David , Corral María J , González-Moya Manuel , Castillo-Trujillo Alfredo , Jimenez-Fonseca Paula , Esteban Emilio , Calderon Caterina 
Published: July 2022

Abstract
Purpose: Cancer and its treatments changes patients’ quality of life. The aim was to analyze quality of life, psychological distress, and life satisfaction in patients with resected versus unresectable cancer.Methods: Two prospective, multicenter (15 medical oncology departments), studies with consecutive patient recluitment were conducted, NEOetic, in individuals with unresectable advanced disease (2020-2021) and NEOcoping, in subjects with resected non-metastatic cancer (2016-2018). Participants completed quality of life (EORTC QLQ-C30), emotional distress (Brief Symptom Inventory, BSI), and life satisfaction (Satisfaction with Life Scale, SWLS) questionnaires before systemic antineoplastic treatment and after treatment. A descriptive, bivariate chi-square analysis and t-tests were performed to ascertain the differences between localized and advanced cancer.Results: A total of 1450 patients were recruited, 941 (65%) with a resected cancer and 509 with unresectable advanced cancer with a mean age of 59.0 and 64.9 years, respectively. The most common cancers were colorectal (42%) and breast (34%) in resected group and bronchopulmonary (29%), colorectal (17%), and pancreatic (11%) in unresectable advanced cancer group. Subjects with advanced disease exhibited worse scores on functional scales (physical, cognitive, emotional, and social), and had more symptoms than those with resected disease prior to initiating systemic treatment. Individuals with advanced cancer displayed better functional status and fewer symptoms, but more fatigue and nausea and those with resected cancer presented worse physical and cognitive function, more psychological distress, and more symptoms post-treatment. Conclusion: This sample reveals how antineoplastic treatment can enhance quality of life for people with unresectable advanced disease and negatively affect individuals with resected, non-metastatic cancer.Structured Abstract: Our study shows the potential quality of life benefit of systemic antineoplastic treatment in patients with unresectable advanced cancer. In addition, it shows the deterioration of quality of life in patients with resected non-metastatic cancer after completion of adjuvant treatment.
Representational momentum of biological motion in full-body, point-light and single-dot displays
Authors: Zucchini Elena , Casile Antonino 
Published: July 2022

Abstract
Observing the actions of others triggers, in our brain, an internal and automatic simulation of its unfolding in time. Here, we investigated whether the instantaneous internal representation of an observed action is modulated by the point of view under which an action is observed and the stimulus type. To this end, we motion captured the elliptical arm movement of a human actor and used these trajectories to animate a photorealistic avatar, a point-light stimulus or a single dot rendered either from an egocentric or an allocentric point of view. Crucially, the underlying physical characteristics of the movement were the same in all conditions. In a representational momentum (RM) paradigm, we then asked subjects to report the perceived last position of an observed movement at the moment in which the stimulus was randomly stopped. In all conditions subjects tended to misremember the last configuration of the observed stimulus as being further forward than the veridical last showed position. This misrepresentation was however significantly smaller for full-body stimuli compared to point-light and single dot displays and it was not modulated by the point of view. We interpret these findings as evidence that full-body stimuli elicit a simulation process that is closer to the instantaneous veridical configuration of the observed movements while impoverished displays (both point-light and single-dot) elicit a prediction that is further forward in time. For all stimulus types, this simulation process seems to be independent from the point of view under which the actions are observed.
A nomogram for predicting postoperative overall survival of patients with lung squamous cell carcinoma: a SEER-based study
Authors: Rao Jin , Yu Yue , Zhang Li , Wang Pei , Wang Xuefu , Wang Zhinong 
Published: July 2022

Abstract

Background: Lung squamous cell carcinoma (LSCC) is a common subtype of non-small cell lung cancer. Our study aimed to construct and validate a nomogram for predicting overall survival (OS) for postoperative LSCC patients. Methods: A total of 8,078 patients eligible for recruitment between 2010 and 2015 were selected from the Surveillance, Epidemiology and End Results database. The study outcomes were 1-, 2-, and 3-year OS. Analyses performed included univariable and multivariable Cox regression, receiver the operating characteristic (ROC) curves, calibration plots, decision curve analysis (DCA), and Kaplan-Meier survival curves.Results: Seven variables were selected to establish the nomogram for LSCC. The area under the curve in ROC curve revealed 0.658, 0.651, and 0.647 in the training cohort and 0.673, 0.667, and 0.658 in the validation cohort at 1-, 2-, and 3-year survival. The calibration curves showed satisfactory consistencies between nomogram-predicted and observed survival probability. DCA showed great clinical net benefit. In risk stratification systems, patients were divided into three risk groups with a significant difference in OS in Kaplan-Meier curves (P＜0.001).Conclusions: A prognostic nomogram for OS of postoperative LSCC patients was developed and validated, which may assist clinicians in evaluating prognosis and providing highly individualized therapy.
The effect of the Interactive Self-talk and Mental Imagery program on Badminton Motor Skills and Self-Confident of Youth Beginner Student- Athletes
Authors: Hidayat Yusuf , Yudiana Yunyun , Hambali Burhan , Sultoni Kuston , Ustun Umit Dogan , Singhnoy Chatkamon 
Published: July 2022

Abstract
Background: self-talk (ST) and mental imagery (MI) psychological strategies have an essential role in training and sports performance, but their implementation is still limited, especially when they are implemented in combination use. This study aimed to examine badminton motor skills (BMS) and self-confidence (SC) mastery after the ST and MI psychological strategy intervention, both in independent and interactive functions integrated into the BMS training process. Methods: Eighty badminton beginner student-athletes aged years 11.4+52 years old (Male=40, Female=40) were divided through a disproportional stratified sampling into four experimental groups (EG: 2-function ST x 2-function MI) and one control group (CG). The intervention program was eight weeks (24 meetings and three times a week). Participants completed a fidelity check at each session. At the end of the intervention, they took a BMS test and completed a self-confidence scale. Results: ST and MI strategies had a significant effect on the BMS and SC mastery (independent and interaction functions), in multivariate and univariate ways, except that, independently, they had no significant effect on SC. The effect of I-ST > M-ST and the effect of C-MI > M-MI on BMS, but there was no different effect on SC. In the M-MI function, the effect of I-ST > M-ST on the BMS and SC mastery. Meanwhile, in the C-MI function, I-ST and M-ST did not have a different interaction effect on BMS and the effect of M-ST > I -ST on SC in the C-MI function. In the M-ST function, the effect of C-MI > M-MI on BMS and SC, but there was no difference of the interaction effect on BMS in the I-ST condition. Ethics approval: Centre of Research and Community Service, Universitas Pendidikan Indonesia (Project No. B-1030/UN40.PL/PJ.00.00/2021), approval date: 25 June 2021.Conclusion: The results of this study strengthen the evidence on the effectiveness of ST and MI strategies in motor skill mastery and psychological skill development of beginner student-athletes. Thus, ST and MI strategies can be adapted as psychological strategies that coaches and physical educators can use to improve beginner student-athlete's learning, sports performance, and psychological skills.
Primary septic arthritis of the knee caused by Neisseria meningitidis serogroup B in an elderly patient. Case report and review of the literature
Authors: Longo Benedetta , Fazio Cecilia , Meini Simone , Neri Arianna , Andreoli Elisabetta , Vacca Paola , Salamone Domenico , Sani Spartaco , Mura Maddalena , Bracalente Irene , Venturini Luigi , Andreini Roberto , Ambrosio Luigina , Carannante Anna , Stefanelli Paola 
Published: July 2022

Abstract
Purpose: Primary meningococcal arthritis (PMA) represents an uncommon clinical presentation of meningococcal infection, mainly reported among young people. Herein, a case of PMA of the knee in an elderly patient is described.Case presentation: On January 2022, an 87-year-old patient arrived at hospital with continuous fever persisting for three days and a picture of joint pain, swelling, redness, and warmth of her left knee. An arthrocentesis was promptly performed and the inoculated synovial fluid turned positive with numerous Gram-negative diplococci at the microscopic examination. The BioFire® FilmArray®Meningitis/Encephalitis (ME) Panel (bioMérieux) quickly confirmed the presence of Neisseria meningitidis. The meningococcal isolate belonged to the serogroup B (MenB), Sequence type (ST)-162/ clonal complex (cc)162. Two grams of ceftriaxone twice a day were administered for 21 days; than cefditoren pivoxil 400 mg twice a day for further 6 weeks after discharge. In Italy, from 2018 to January 2022, among 135 MenB, 31 MenB/cc162 were identified, of which only the case here reported was associated with an atypical clinical picture.Review of the literature: A total of 40 cases of PMA caused by N. meningitidis was reported in the literature, but only 4 occurred in elderly. To our knowledgements, no cases of PMA caused by MenB were previously reported among patients of more than 65 years of age.
A Novel Compartmental Approach to Modeling COVID-19 Disease Dynamics and Analyzing the Effect of Common Preventative Measures
Authors: Lin Caden 
Published: July 2022

Abstract
As of December 2020, the COVID-19 pandemic has infected over 75 million people, making it the deadliest pandemic in modern history. This study develops a novel compartmental epidemiological model specific to the SARS-CoV-2 virus and analyzes the effect of common preventative measures such as testing, quarantine, social distancing, and vaccination. By accounting for the most prevalent interventions that have been enacted to minimize the spread of the virus, the model establishes a paramount foundation for future mathematical modeling of COVID-19 and other modern pandemics. Specifically, the model expands on the classic SIR model and introduces separate compartments for individuals who are in the incubation period, asymptomatic, tested-positive, quarantined, vaccinated, or deceased. It also accounts for variable infection, testing, and death rates. I first analyze the outbreak in Santa Clara County, California, and later generalize the findings. The results show that---although all preventative measures reduce the spread of COVID-19---quarantine and social distancing mandates reduce the infection rate and subsequently are the most effective policies, followed by vaccine distribution and, finally, public testing. Thus, governments should concentrate resources on enforcing quarantine and social distancing policies. In addition, I find mathematical proof that the relatively high asymptomatic rate and long incubation period are driving factors of COVID-19’s rapid spread.
Paving the way for synthetic C1- metabolism in Pseudomonas putida through the reductive glycine pathway
Authors: Bruinsma Lyon , Wenk Sebastian , Claassens Nico , Santos Vitor Martins dos 
Published: July 2022

Abstract

One-carbon (C1) compounds such as methanol, formate, and CO2 are alternative, sustainable microbial feedstocks for the biobased production of chemicals and fuels. In this study, we engineered the carbon metabolism of the industrially important bacterium Pseudomonas putida to assimilate these three substrates through the reductive glycine pathway. First, we demonstrated the functionality of the C1-assimilation module by coupling the growth of auxotrophic strains to formate assimilation. Next, we extended the module from formate to methanol using both NAD and PQQ – dependent methanol dehydrogenases. Finally, we demonstrated CO2-dependent growth through CO2 reduction to formate by the native formate dehydrogenase, which required short-term evolution to rebalance the cellular NADH/NAD+ ratio. This research paves the way to engineer P. putida towards growth on formate, methanol, and CO2 as sole feedstocks, thereby substantially expanding its potential as a sustainable and versatile cell factory.
Psychological resilience mediates the association between childhood maltreatmentand self-harm phenotype in Chinese early adolescents
Authors: Li Yuan , Li Yong-Han , He Yang , Chen Shan-Shan , Chang Jun-Jie , Yuan Meng-Yuan , Cao Lei-Lei , Wang Shao-Jie , Wang Geng-Fu , Su Pu-Yu 
Published: July 2022

Abstract

BackgroundSelf-harm (SH) increases significantly in early adolescence with great variability, and childhood maltreatment (CM) contributes to this increase. Understanding the developmental pathway from CM to SH could provide clues for SH prevention. This study used latent class analysis (LCA) to detect the phenotype of SH and explored the role of psychological resilience in the pathway from the CM to SH phenotype.MethodsA cross-sectional study was conducted with 5724 early adolescents from three middle schools in Anhui Province, China. We categorized SH into five subtypes based on their severity and further explored SH phenotypes by LCA to detect the heterogeneity of SH. Moreover, a path model was performed to test the mediation of resilience in the CM-SH association.ResultsThe prevalence rates of highly lethal SH, less lethal SH with visible tissue damage, SH without visible tissue damage, self-harmful behaviours with latency damage and psychological SH were 10.2%, 25.8%, 35.5%, 20.8% and 28.2%, respectively. Three interpretable phenotypes of SH were identified: low SH (57.8%), medium SH (29.0%), and high SH (13.2%). Furthermore, CM was positively associated with the SH phenotype, psychological resilience mediated the association between CM and the SH phenotype (all ps < 0.01), and a larger mediating effect was observed in the medium SH (22.41%).ConclusionsOur findings offer new perspectives that improving psychological resilience can be used as an efficient intervention to reduce the risk of SH among early adolescents who have experienced CM.
BMI and pelvimetry help to predict the duration of laparoscopic resection for low and middle rectal cancer
Authors: Teng Wenhao , Liu Jingfu , Chen Meimei , Zang Weidong , Wu Aiwen 
Published: July 2022

Abstract
Background: In rectal cancer surgery, recent studies have found associations between clinical factors, especially pelvic parameters, and surgical difficulty; however, their findings are unreliable because the studies use different criteria. This study aims to evaluate common clinical factors that influence operative time for the laparoscopic anterior resection of low and middle rectal cancer.Methods: Patients who underwent laparoscopic radical resection of low and middle rectal cancer from January 2018 to December 2020 were retrospectively analyzed and classified according to operative time. Preoperative clinical and MRI-related parameters were collected. Logistic regression analysis was used to identify factors for predicting operative time.Results: In total, 214 patients with a mean age of 60.3±8.9 years were divided into two groups: the long operative time group (n=105) and the short operative time group (n=109). Univariate analysis revealed that male sex, higher BMI(≥24.0 kg/m2), preoperative treatment, smaller pelvic inlet (<11.0 cm), and shorter pelvic depth(<10.7 cm) were significantly correlated with longer operative time(P<0.05). However, only BMI(OR=1.893,95% CI 1.064-3.367, P=0.030) and pelvic inlet (OR=0.439,95% CI 0.240-0.804, P=0.008) were independent predictors of longer operative time. Moreover, the rate of anastomotic leakage was higher in the group with a longer operative time (P<0.05).Conclusion: A longer surgical duration might be associated with more anastomotic leakage; however, surgical duration was predictable when considering BMI and pelvic inlet. Laparoscopic rectal resection patients with higher BMI or smaller pelvic inlets are expected to take longer to perform. This finding is helpful for centers that lack surgical resources; by taking it into consideration, these centers can make more reasonable surgical arrangements and prepare for exceptional postoperative management.
The benefit of treatment beyond progression with immune checkpoint inhibitors: A multi- center study
Authors: Guven Deniz Can , Yekeduz Emre , Erul Enes , Yazgan Sati Coskun , Sahin Taha Koray , Karatas Gokturk , Aksoy Sercan , Erman Mustafa , Yalcin Suayib , Urun Yuksel , Kilickap Saadettin 
Published: July 2022

Abstract
Objective: Treatment beyond progression (TBP) with immune checkpoint inhibitors (ICIs) is an evolving field due to the limitations of conventional imaging in response evaluation with ICIs. However, real-life data on the benefit of TBP is scarce, especially from the limited resource settings. Therefore, we aimed to investigate the survival benefit of TBP with ICIs in patients with advanced tumors from a limited resource setting.Methods: For this multicenter study, we included 282 patients treated with ICIs and radiological progression according to RECIST 1.1 criteria. The evaluated post-progression survival according to the use of TBP (TBP and non-TBP groups) with univariate and multivariate analyses.Results: 82 (29.1%) of 282 patients continued on ICIs following the initial progression event. In multivariate analyses, patients in the TBP group had improved post-progression survival compared to non-TBP (13.18 vs. 4.63 months, HR: 0.500, 95% CI: 0.349-0.717, p<0.001). The benefit of the TBP was independent of the tumor type, treatment line, and patient age. Furthermore, TBP with ICIs was associated with improved post-progression survival (HR: 0.600, 95% CI: 0.380-0.947, p=0.028) in the additional analyses after excluding the patients with no further treatment after progression in the non-TBP arm.Conclusions: In this study, we observed that patients receiving ICIs beyond progression had considerably longer survival. Continuation of ICIs after progression should be kept in mind as a reasonable management option for patients with advanced cancer, specifically for patients with limited alternative options.