bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of dexmedetomidine up-regulating HIF-1ɑ on intestinal mucosal barrier injury related to sepsis in rats
Authors: Lei Xiuzhen , Zhao Bihan , Li Jun , Huang Suqin , Li Yuhong , Zhu Shengmei 
Published: March 2022

Abstract
Dexmedetomidine (DEX), a highly selective α2 receptor agonist. DEX can inhibit the release of inflammatory factors in septic rats. It can also protect against H2O2 injury by up-regulating HIF-1α expression. The aim of this study was to explore whether DEX can up regulate HIF-1ɑ protein level and plays a role in reducing septic intestinal mucosal injury. Twenty-four Sprague Dawley (SD) rats were randomly divided into four groups of 6 rats each: the sham group, sepsis group (subjected to cecal ligation and perforation, CLP), sepsis + dexmedetomidine group (DEX group, 30 µg/kg of DEX by intraperitoneal injection 30 minutes before and 2 hours after CLP), and sepsis + DEX + HIF-1ɑ inhibitor Bay87-2243 (Bay87-2243 group, 30 µg/kg of DEX by intraperitoneal injection 30 minutes before and 2 hours after CLP and 9mg/kg of bay87-2243 by oral administration for 3 days before CLP. The HIF-1ɑ and the tight junction protein (TJs) was detected by Western blot; the plasma concentrations of diamine oxidase (DAO), intestinal fatty acid binding protein (FABP2) and D-lactic acid (D-LAC) were detected by ELISA; the morphological changes of intestinal mucosa were detected by HE staining. DEX significantly increased the expression level of HIF-1ɑ on intestinal mucosa in rats with sepsis injury, thus ameliorated intestinal mucosal pathological injury, reduced Chiu’s score, decreased intestinal mucosal permeability, and up-regulated TJs protein expression. Moreover, effect on sepsis induced intestinal mucosal injury of DEX was reversed by HIF-1ɑ inhibitor Bay87-2243. DEX could protect against sepsis-induced intestinal mucosal injury by up-regulating HIF-1ɑ expression in rats.
Scientific evidence for the cancer treatment of human-natured infrared radiation and stimulated electron radiation
Authors: Erdene-Ochir Enkhmaa , Jamiyan Batsuuri , Chinbat Munkhbaatar 
Published: March 2022

Abstract
The principles of surgery in the early stages of cancer, physical radiation in severe cases, and chemotherapy are now commonplace around the world. When the cancer has spread (metastasis) within the organ of its first formation and to other parts of the body, these methods can increase life expectancy by a maximum of five years. At the current level of scientific progress, no perfect cure for cancer has yet been found. Here, we demonstrate the result of our study of how Purevsuren Begzjav, a Mongolian citizen and healer, uses her natural bioenergy to completely cure late-stage cancer. It was found that B.Purevsuren’s palms (body) emit hot photons of stimulated electrons saturated with infrared light at 40°C to 45°C, which regenerate the patient’s bioplasma (energy) body and restrain the cancer foci and eradicate it. B.Purevsuren’s innate infrared energy reaches the organs and organ systems of the human body in appropriate doses, is harmless, and the level of her bioenergy parameters is quite stable. For example, the mean pre-treatment bioenergy area of the healer (X̅ ± S = 11549.3 ± 672.3) is maintained at 98.4 percent compared to the post-treatment average (X̅ ± S = 11368.0 ± 650.2). However, the mean pre-treatment bioenergy intensity of the healer (X̅ ± S = 75.42 ± 1.48) is maintained at 99.6 percent compared to the post-treatment average (X̅ ± S = 75.14 ± 1.53). The result of B. Purevsuren’s treatment is providing evidence that there is a scientific approach to cure cancer with photons of human-natured, high-temperature infrared energy-stimulated electrons completely. Trial registration (NIRT20121127), November 27, 2012
Influence of Prebiotics and Bacteriocin on Yogurt Quality
Authors: Hussien Heba , Abd-Rabou Hagar Saeed , Saad Marwa A. 
Published: March 2022

Abstract
This study determined the quality of yogurt fortified with 2% inulin, 2% Fructo-oligo-saccharides (FOS), and Lactobacillus acidophilus with their bacteriocins. The results indicated that coagulation time increased by 3.29–22.75% in yogurts prepared with Lactobacillus acidophilus and their bacteriocins compared to the control also titratable acidity increased gradually in all groups during storage. The sensorial properties showed the superiority of inulin, FOS, and Lactobacillus acidophilus bacteriocin groups. Lactobacillus bulgaricus, Streptococcus thermophiles, and Lactobacillus acidophilus count increased in the treatments compared to the control group incorporated with an extended shelf life to 39 days of storage in the treatments containing lactobacillus acidophilus bacteriocin. Coliforms, Moulds, and yeasts did not detect in the group containing 2% inulin, 2% FOS, and lactobacillus acidophilus bacteriocin for 21 days of refrigerated storage. This study proved that 2% inulin, 2% FOS, and Lactobacillus acidophilus bacteriocin fortification extended the shelf life for 6 weeks.
A Wearable Fiber-free Optical Sensor for Continuous Monitoring of Cerebral Blood Flow in Freely Behaving Mice
Authors: Liu Xuhui , Irwin Daniel , Huang Chong , Gu Yutong , Chen Li , Donohue Kevin , Chen Lei , Yu Guoqiang 
Published: March 2022

Abstract
Wearable technologies for functional brain monitoring in freely behaving subjects would advance our understanding of cognitive processing and adaptive behavior. Existing technologies are lacking in this capability or need procedures which are invasive and/or otherwise impede brain assessments during social behavioral conditions, exercise, and sleep. In response we developed a complete system combining cerebral blood flow (CBF) measurement, O2 and CO2 supplies, and behavior recording for use on conscious, freely behaving mice. An innovative diffuse speckle contrast flowmetry (DSCF) device and associated hardware were miniaturized and optimized for small subject applications. The installation and use of this wearable, fiber-free, near-infrared DSCF head-stage/probe required no craniotomy, invasive probe implantation, or restraints of awake animals. Significant correlations were found between measurements with the new DSCF design and an optical standard. The system successfully and repeatedly detected CBF responses to CO2-induced hypercapnia in both anesthetized and moving mice. Collecting CBF and activity information together during natural behaviors provides realistic physiological results and opens the path to exploring their correlations with pathophysiological conditions.
A new treatment option for stage IIIB lung squamous cell carcinoma-Hypofractionated brachytherapy combined with ICIs: A Case Report
Authors: Wei Chun , Cui Ran , Li Xian Yi , Jiang Ou 
Published: March 2022

Abstract
Here, we present a case of a patient with stage IIIB squamous lung cancer with mediastinal and cervical lymph node metastases in the left lung with peripheral squamous lung cancer, who was selected for simultaneous immunotherapy with insertional radiotherapy at the primary site in the left lung, followed by intensity-modulated radiotherapy for mediastinal and cervical lymph node metastases and maintenance immunotherapy every 3 weeks. There was no treatment-related toxicity above grade 2 during the treatment. The response was PR, and PFS was more than 12 months. That provides a new therapeutic idea for treating advanced squamous lung cancer. Here, we present a case of a patient with stage IIIB squamous lung cancer with mediastinal and cervical lymph node metastases in the left lung with peripheral squamous lung cancer, who was selected for simultaneous immunotherapy with insertional radiotherapy at the primary site in the left lung, followed by intensity-modulated radiotherapy for mediastinal and cervical lymph node metastases and maintenance immunotherapy every 3 weeks. There was no treatment-related toxicity above grade 2 during the treatment. The response was PR, and PFS was more than 12 months. That provides a new therapeutic idea for treating advanced squamous lung cancer.
Predicting Clinical Outcomes of Alpha-1 Antitrypsin Deficiency-Associated Liver Disease Using a Stacking Ensemble Machine Learning Model Based on UK Biobank Data
Authors: Meng Linxi , Treem Will , Heap Graham , Chen Jingjing 
Published: March 2022

Abstract
With many unknowns in alpha-1-antitrypsin deficiency-associated liver disease (AATD-LD), we aimed to develop a tailored stacking ensemble supervised machine learning (ML) model to predict the disease progression and clinical outcomes of AATD-LD to enable the data-driven decision-making for the clinical outcome endpoints selection as well as clinical development strategy. This analysis was carried out through a stacking ensemble learning model via meta-learning by combining five different supervised ML algorithms with 58 potential predictor variables using a nested 5-fold cross-validation with repetitions based on the UK Biobank data. Performance of the model was assessed through prediction accuracy, area under the receiver operating characteristic (AUROC), and area under the precision-recall curve (AUPRC). The importance of predictor contributions was evaluated through a feature importance permutation method. For example, the AUROC of the prediction model in patients with AATD-LD was 68.1%, 75.9%, 91.2%, and 67.7% for all-cause mortality, liver-related death, liver transplant, and all-cause mortality or liver transplant, respectively. The generalizable predictive patterns support the use of ML to address the unanswered clinical questions with clinically meaningful accuracy using real-world data. This method can be easily applied to other clinical outcomes and/or diseases.
Utilization of Long –Acting Contraceptive Methods and Associated Factor Among Female Health Care Providers in South Wollo Zone Hospitals, North East, Ethiopia.
Authors: seid Mehdi , Yimer Aragaw , seid Ahmed , Fantaye Fasil , Damtie Yitayshi 
Published: March 2022

Abstract
Back ground: - Long-acting contraception method utilization was found low (22.7%) among female health care providers. However, as far as my literature search no study was done on long-acting contraception methods utilization among female health providers in the study area. These studies failed to include important variable like socio demography and individual factor which might affect the use of long-acting contraceptive methods female health providers and limited studies in Ethiopia. Objective: - To assessing utilization of long -acting contraceptive methods and associated factors among health care providers in south wollo zone public hospitals, Amhara region, Ethiopia 2021.Methods: -An Institutional-based cross- sectional was conducted among 354 female health care workers in south wollo zone hospitals, from March to April 22, 2021. The participants were selected using Systematic random sampling technique. Data were collected by using self-administered questionnaire. The data were entered Epi-data version 4.1 and export to SPSS versions 25 for analysis. Bivariable and multivariable logistic regression analysis were performed. The Adjusted odds Ratio (AOR) along with a 95% confidence interval (CI) was estimated to measure the association and level of significance was declared at a P- value of less than 0.05.Result: -The current utilization of long-acting contraceptive methods among female health care providers was found to be 33.6% [95%, CI 29-39)], discuss with partner [AOR= 2.277,95% CI, (1.026-5.055)], method shift/switched [AOR=4.302,95% CI, (2.285-8.102)], knowledge of respondent [AOR= 1.887,95% CI, (1.020-3.491)], ever birth [AOR=15.670,95% CI, (5.065-48.49)] were significant factors towards the utilization of long-acting contraceptive methods.Conclusion :The current utilization of long-acting contraceptive methods was found low. Discus with partner, methods shift/switching, ever birth and knowledge of respondents were identified as significant factor. Therefore, encouraging partner discussion targeted Information Education Communication Intervention should be intensified to improve long-acting contraceptive methods utilization.
Post-recurrence survival analysis in patients with oligo-recurrence after curative esophagectomy
Authors: Hsu Po-Kuei , Tsai Ping-Chung , Chien Hung-Che , Hung Jung-Jyh , Huang Chien-Sheng , Hsu Wen-Hu , Hsu Han-Shui 
Published: March 2022

Abstract
BackgroundRecurrent esophageal cancer is associated with dismal prognosis. There is no consensus about the role of surgical treatments in patients with limited recurrences. This study aimed to evaluate the role of surgical resection in patients with resectable recurrences after curative esophagectomy and to identify their prognostic factors. MethodsWe retrospectively reviewed patients with recurrent esophageal cancer after curative esophagectomy between 2004 and 2017 and included those with oligo-recurrence that was amenable for surgical intent. The prognostic factors of overall survival (OS) and post-recurrence survival (PRS), as well as the survival impact of surgical resection, were analyzed. ResultsAmong 654 patients after curative esophagectomies reviewed, 316 (48.2%) had disease recurrences. The recurrences were found resectable in 63 (9.6%) patients, and 30 (4.6%) patients received surgery. The significant prognostic factors of PRS with poor outcome included mediastinum LN recurrence and pathologic T3 stage. In patients with and without surgical resection for recurrence cancer, the 3-year OS rates were 65.6% and 47.6% (p = 0.108), while the 3-year PRS rates were 42.9% and 23.5% (p = 0.100). In the subgroup analysis, surgery for resectable recurrence, compared with non-surgery, could achieve better PRS for patients without any comorbidities (hazard ratio 0.36, 95% CI: 0.14 to 0.94, p = 0.038).ConclusionsMediastinum LN recurrence or pathologic T3 was associated with worse OS and PRS in patients with oligo-recurrences after curative esophagectomies. No definite survival benefit was noted in patients undergoing surgery for resectable recurrence, except in those without comorbidities.
Controlling the optical properties and analyzing mechanical, dielectric characteristics of MgO doped (PVA–PVP) blend by altering the doping content for multifunctional microelectronic devices
Authors: Mohammed M.I. 
Published: March 2022

Abstract
The solution-cast method was used to synthesize magnesia (MgO) nanoparticles filled poly(vinyl alcohol) (PVA) and poly(vinyl pyrrolidone) (PVP) blend matrix (80/20 wt %) based polymeric nanocomposite (PNC) films (i.e., [PVA–PVP]–x wt % MgO; x = 0, 0.06, 0.3, 0.6, 3.1, and 6.25). Most of these PNC materials structures are amorphous, according to an X-ray diffraction investigation. The blend surface was smooth and homogeneous under SEM, confirming PVA and PVP compatibility. MgO loading, on the other hand, enhanced the surface roughness. According to optical investigations, the films transmittance, Urbach's energy, and energy bandgap decreased as the Mg+ 2 -ions increased. Tensile strength has also improved from 5.92 MPa to 10.65 MPa, while Young's modulus has enhanced from 51.16 to 166.4 MPa. From 100 Hz to 1 MHz, the dielectric and electrical spectra of these films were measured. Due to the nanoconfinement effect, it has been determined that the dispersion of MgO nanoparticles in the PVA–PVP blend matrix dramatically increases the dielectric permittivity. With increasing frequency, the dielectric permittivity of these PNC films decreases while the ac electrical conductivity increases. When the temperature of a PNC film is raised, a nonlinear rise in dielectric permittivity is seen, and the dc electrical conductivity of the film follows the Arrhenius law. The nonlinear I-V curves improve with increased Mg+ 2 ion concentration. The developed and engineered unique high-performance flexible nanocomposites in the field of advanced functional materials for usage in next-generation optoelectronic, gas sensors, and microelectronic devices were ascribed to the studied properties of the PNC films.
Causal inference of genetic variants and genes in amyotrophic lateral sclerosis
Authors: Pan Siyu , Liu Xinxuan , Liu Tianzi , Zhao Zhongming , Dai Yulin , Wang Yin-Ying , Jia Peilin , Liu Fan 
Published: March 2022

Abstract
Amyotrophic lateral sclerosis (ALS) is a fatal progressive multisystem disorder with limited therapeutic options. Although genome-wide association studies (GWASs) have revealed multiple ALS susceptibility loci, unraveling causal relationships between genetic variants, genes, cell types, tissues, and risk of ALS remains challenging. Here, we reported a comprehensive post-GWAS analysis of the recent large ALS GWAS (n = 80610), including functional mapping and annotation (FUMA), transcriptome-wide association study (TWAS), colocalization (COLOC) and summary da-ta-based Mendelian Randomization analyses (SMR) in extensive multi-omics datasets. Gene property analysis highlighted inhibitory neuron 6, oligodendrocytes and GABAergic neurons (Gad1/Gad2) as functional cell types of ALS and confirmed cerebellum and cerebellar hemisphere as functional tissues of ALS. Functional annotation detected the presence of multiple deleterious variants at 3 loci (9p21.2, 12q13.3, 12q14.2) and highlighted a list of SNPs that are potentially functional. TWAS, COLOC, and SMR identified 43 genes at 24 loci, including 23 novel genes and 10 novel loci, showing significant evidence of causality. Integrating multiple lines of evidence, we further propose that rs2453555 at 9p21.2 and rs229243 at 14q12 functionally contribute to the development of ALS by regulating the expression of C9orf72 in pituitary and SCFD1 in skeletal muscle, respectively. Overall, our study represents the most comprehensive post GWAS study of ALS to date and provides important new insights into the etiology of ALS.
Effects of Glutamate and γ-aminobutyric Acid of Cerebral Ischemia on Rhesus Monkey
Authors: ZHANG Xiao-ran , Jun PU , LIU Bo-hu , LI ze-qi 
Published: March 2022

Abstract
Objective To explore the effect of cerebral ischemia in rhesus monkeys on the metabolism of glutamate and γ-aminobutyric acid. Method Bilateral common carotid artery was blocked in 9 rhesus monkeys. The extracellular fluid was collected by microdialysis. Perform liquid chromatography analysis . Result After cerebral ischemia, the content of glutamate and gamma-aminobutyric acid was significantly increased compared with that of ischemia. P <0.05, the difference was statistically significant. Conclusions Cerebral ischemia hypoxia leads to the large release of glutamate, which leads to increased synthesis of GABA and antagonism to it’s excitatory toxicity.
Mutations in α-synuclein, TDP-43 and tau prolong protein half-life through diminished degradation by lysosomal proteases
Authors: Kao Aimee , Sampognaro Paul , Arya Shruti , Knudsen Giselle , Gunderson Emma , Sandoval-Perez Angelica , Craik Charles , Jacobson Matthew 
Published: March 2022

Abstract
Autosomal dominant mutations in α-synuclein, TDP-43 and tau promote protein aggregation and neurodegeneration. Rates of aggregation are highly dependent on protein concentration, which can be regulated via lysosomal proteolysis. Lysosomal cathepsins recognize specific linear amino acid sequences; thus, mutations in α-synuclein, TDP-43 and tau have the potential to impact protein half-life by impairing lysosomal degradation. To test this, we first generated comprehensive proteolysis maps containing cathepsin cleavage sites for each of these disease-associated proteins. In silico analysis of these maps suggested that certain mutations would diminish cathepsin cleavage, a prediction we validated utilizing in vitro protease assays. We further demonstrated that lysosomes degrade inducibly-expressed mutant forms of α-synuclein, TDP-43 and tau less efficiently than wild-type protein. Together, this study provides evidence that pathogenic mutations in α-synuclein, TDP-43 and tau directly impair their own lysosomal degradation, altering protein homeostasis by increasing the half-life of these proteins.
Absolute quantification of gene copy number variation in Plasmodium falciparum novel putative genes associated to drug resistance (the actin-binding coronin protein, cysteine desulfurase and plasmepsin 2), using Eva green-based quantitative PCR
Authors: Diarra Houdou , Mobegi Victor A. , Makhulu Edward E. , Kinyua Johnson , Herren Jeremy K 
Published: March 2022

Abstract
Background: Exposure of Plasmodium falciparum to host immune system and antimalarials pressure leads the parasite to exploit genetic factors such as genome sequence variation (structural variation and single nucleotide polymorphism) to withstand environmental conditions. Gene copy number variation (CNVs) is a type of genome sequence polymorphism and it has been shown that CNVs contribute to parasite adaptation to environmental pressure, acquisition of drug resistance and enable the parasite to escape from host immune system. In this study, we explored CNVs in P.falciparum three putative genes related to drug pressure.Method: Blood samples were obtained from asymptomatic school children collected from December 2016 to October 2018 in Mbita sub-county, western Kenya. Genomic DNA was extracted using ISOLATE II Genomic DNA kit (Bioline, UK) following the manufacturer’s instructions. Real-time quantitative PCR was performed and the target genes copy numbers were determined by absolute quantification method.Results: The findings revealed differential level of copy number variation in target genes investigated. One isolate (KE024/18) was found to contain 5.29 copies of Pfcoronin gene, t(2) = 27.91, P = 0.0013, 95% CI [3.62 - 4.95]. In the Pfcysteine desulfurase gene, similar level of copy amplification was observed in the OS0149/18, (5.09 copies) t(2) = 7.85, P = 0.0148, 95% CI [1.85 – 6.33]. These two isolates were found to harbour the highest level of CNVs of the corresponding genes. Pfplasmepsin 2 gene was found to be less polymorphic, however, three isolates showed high copy amplification, OS062/17 (9.63 copies), t(2) = 80.50, P = 0.0002, 95% CI [8.01 – 9.09]; AL106/16 (9.18 copies), t(2) = 28.61, P = 0.0012, 95% CI [6.95 – 9.41], and KM019/18 (9.41 copies), t(2) = 90.51, P = 0.0001, 95% CI [8.01 – 8.81].Conclusion: The results show gene copy number variation in the clinical samples we explored. The findings suggest that P.falciparum may be under drug pressure in the study area, therefore, this calls for close surveillance of drug resistance.
Paralytic Ileus as First Symptom of Miller Fisher Syndrome: a Case Report
Authors: Liu Xiubin , Chen Xiqi , Zhang Xiaoxia , Zhou Yongkun 
Published: March 2022

Abstract
Introduction Miller Fisher syndrome (MFS) is a nervous disease, immune correlation of Guillain Barre syndrome (GBS).The typical clinical manifestations of MFS are extraocular muscle paralysis, ataxia, tendon reflex loss or disappearance. This report describes a case of MFS with paralytic ileus as the first symptom. Case presentation A 48-year-old woman presenting with abdominal pain and distention was diagnosed with paralytic ileus. There was no significant improvement in symptoms after symptomatic treatment. After that, the patient developed visual rotation, with limited binocular abduction and adduction, and ataxia. Anti-ganglioside testing revealed positive anti-GQ1b antibodies. The patient was diagnosed as Miller Fisher syndrome. And recovered after symptomatic treatment. Conclusion In case of inconsistency between imaging examination and clinical symptoms, neuroelectrophysiology and cerebrospinal fluid puncture should be performed in time, and strive for timely detection and treatment.