bluuebunny/crossref_metadata_2025_split huggingface dataset:
Melatonin reduces muscle damage, inflammation and oxidative stress induced by exhaustive exercise in people with overweight/obesity
Authors: Ben Dhia Imen , Maaloul Rami , Marzougui Houssem , Ghroubi Sameh , Kallel Choumous , Driss Tarak , Elleuch Mohamed Habib , Ayadi Fatma , Turki Mouna , Hammouda Omar 
Published: March 2022

Abstract

Background
Intense physical exercise leads to inflammation, oxidative stress and muscle damage, and these responses are of greater magnitude in people with obesity. Melatonin (MLT) is considered an endogenous antioxidant which may have beneficial effects against inflammation, oxidative stress and promote tissue repair after exercise. The aim of this study was to examine the effect of MLT on inflammatory parameters, oxidative stress and muscle damage in people with overweight/obesity after a high-intensity interval exercise (HIIE).

Methods
A total of 23 subjects with obesity (9 men and 14 women) age: 33.26 ± 9.81 years, BMI: 37.75 ± 8.87 kg.m−2 were randomized to participate in two experimental sessions: HIIE + Placebo and HIIE + MLT (3 mg). The HIIE protocol corresponds to 8 intervals of 1 min (90% of the maximal aerobic power (MAP)) alternating with 2 min recovery (45% of the MAP). Blood samples were drawn before and 5 min after each exercise session.

Results
MLT ingestion attenuated the increase of inflammation (C-reactive protein, white blood cells (P < 0.001, ηp2 = 0.45; for both) and Neutrophils (P < 0.01, ηp2 = 0.36)) and hepatic and muscle damage (Aspartate aminotransferase (P < 0.01, ηp2 = 0.25), Alanine aminotransferase (P < 0.01, ηp2 = 0.27) and Creatine kinase (P = 0.02, ηp2 = 0.23). MLT also attenuated the exercise induced lipid and protein peroxidation (i.e., Malondialdehyde (P = 0.03, ηp2 = 0.19) and AOPP (P < 0.001, ηp2 = 0.55)). Concerning the antioxidant status, MLT intake increased Thiol (P < 0.01, ηp2 = 0.26) and Catalase (P < 0.01, ηp2 = 0.32) and decreased Uric acid (P = 0.02, ηp2 = 0.2) and Total bilirubin (P < 0.01, ηp2 = 0.33).

Conclusions
MLT intake before HIIE reduced muscle damage by modulating oxidative stress and preventing overexpression of the pro-inflammatory mediators in people with obesity.
Metabolic syndrome in patients with COPD: Causes and pathophysiological consequences
Authors: Fekete Monika , Szollosi Gergo , Tarantini Stefano , Lehoczki Andrea , Nemeth Anna N , Bodola Csenge , Varga Luca , Varga Janos Tamas 
Published: March 2022

Abstract

Background
Decreased physical activity significantly increases the probability of prevalent metabolic syndrome (MetS) with substantial impact on the expected course of COPD.

Objective
Our research aims to assess the metabolic consequences of chronic obstructive pulmonary disease (COPD) and evaluate the prevalence of MetS and its interrelations with age, sex, comorbidities, drug intake, degree of decreased lung function, nutritional status, physical activity and quality of life.

Methods
A cross-sectional study was performed on a random sample (n = 401) at the Department of Pulmonary Rehabilitation of the National Koranyi Institute of Pulmonology from March 1, 2019 to March 1, 2020 in Budapest, Hungary. Anthropometric and respiratory function tests and laboratory parameters of all patients were registered.

Results
MetS occurred in 59.1% of COPD patients with significant gender difference (male: 49.7% female: 67.6%). Concerning BMI, the prevalence of MetS was higher with BMI≥25 kg m−2 (P < 0.0001). Patients with this syndrome had significantly worse FEV1%pred (43 (30–56) vs. 47 (36–61); P = 0.028), lower quality of life (CAT: 26 (21–32) vs. 24.5 (19–29); P = 0.049) and significantly more frequent exacerbations (2 (1–3) vs.1 (0–2); P < 0.05), than patients without MetS. The prevalence of comorbidities were higher in overweight/obese patients (BMI> 25 kg m−2).

Conclusions
In COPD patients MetS negatively affect respiratory function and quality of life and promotes exacerbations of the disease. MetS is related to nutritional status and the level of systemic inflammation in COPD patients.
Effect of resistance exercise with arm sleeve compression garments accelerates blood lactate removal
Authors: Chiappa Gaspar Rogerio da Silva 
Published: March 2022

Purpose:
It has long been suggested that arm sleeve compression garments (CG) may impact blood lactate levels ([Lac-]B) during the recovery from resistive exercise. In this study, we tested the hypothesis that upper-body compression garments during recovery from intense resistive exercise contribute to La clearance, thus leading to reduced [Lac-]B.
Methods:
Sixteen healthy men underwent two sequences of flexion and extension exercises of the elbows and triceps on a pulley, with and without upper-body compression garments, separated by 72h. The exercises were performed with 3 sets of 10 RM, and a cadence of 2 sec was maintained in the eccentric phase and concentric phase. During an inactive recovery period of 20 minutes, serial arterialized venous blood samples were collected to obtain lactate concentrations.
Results:
Subjects showed similar responses at baseline and at peak exercise during the two experimental conditions. [Lac-]B during recovery was reduced with the use of upper-body compression garments at 5, 10, 15, and 20 min of recovery (P<0.05).
Conclusion:
These data are consistent with the notion that compression garments can accelerate lactate removal during recovery from intense exercise.
Protective effect of Crocus sativus stamens extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney
Authors: MAMRI Samira , DAOUDI Nour Elhouda , MARGHICH Mohamed , OUAHHOUD Sabir , KHOULATI Amine , CHOUKRI Mohammed , ASEHRAOU Abdeslam , BNOUHAM Mohamed , SAALAOUI Ennouamane 
Published: March 2022

Crocus sativus is a medicinal plant supposedly possessing various biological activities. Currently, it is evaluated only by the medicinal properties of its stigma and many parts of this plant are unused. This work contributes to the valorization of C.sativus stamens by exploring the property of methanolic extract to prevent gentamicin-induced nephrotoxicity in rats. Twenty Wistar rats (weight 250 ± 30g) were assigned into four equal groups (n = 5), and among the assigned groups, group 1 was given only distilled water (Control), group 2 received intraperitoneal (i.p.) injection of gentamicin (GEN) 80 mg/kg/d, group 3 received the combination of gentamicin (80 mg/kg/d, i.p.) and oral administration of a lower dose of C. sativus methanolic extract (250 mg/kg/d), while the group 4 received the combination of gentamicin (80 mg/kg/d, i.p.) and oral administration of a higher dose of C. sativus methanolic extract (500 mg/kg/d). The injection of gentamicin for the nephrotoxicity induction and post-treatment with methanolic extract was carried out once a day for 15 days. For nephrotoxicity evaluation, biochemical and histopathological analyses were performed. The estimation of serum and urinary creatinine, blood urea nitrogen, sodium levels was carried out with the help of Architect Ci 4100 Analyzer. Oxidative stress was assessed by the determination of renal malondialdehyde (MDA) and catalase (CAT) levels. The results of the study suggested that gentamicin injection induced a significant (p < 0.01) elevation in serum renal biochemical parameters and oxidative stress indices. The methanolic extract of C. sativus significantly (p < 0.05) reduced serum creatinine, urea, and sodium levels, with an improvement in the histopathological results of gentamicin-induced alterations. Furthermore, pretreatment with plant extracts improved hepatic antioxidant status, by the elevation of the CAT and reducing the lipid peroxidation level (MDA) in tissues. The present study suggests that the methanolic extract of C. sativus stamens has an interesting nephroprotective effect on the renal lesions induced by GEN in modulating renal parameters and oxidative stress on Wistar rats.
Effect of Short-term Irrigation of Treated Wastewater on Vetch (Vicia sativa L.) and Alfalfa (Medicago sativa L.) Growth
Authors: Qaoud Hassan Abu , Shtaya Munqez , Mizyed Numan , Al-Fares Heba , Lebdi Ahmed 
Published: March 2022

Background: This study was conducted to evaluate the production of two fodder crops; common vetch (Vicia sativa L.) and Alfalfa (Medicago sativa L.) irrigated with treated wastewater. The study was implemented during summer 2015. Methods: Four water regimes were used in the experiment including: Irrigation with fresh water (control), fresh water supplied by 10 ppm N-P-K fertilizer, treated wastewater and treated wastewater supplied with 10 ppm N-P-K fertilizer. The crops were planted in 5 liters plastic pots in 4:1 v/v sandy to clay soil. The soil was analyzed before and after planting. The following plant parameters were recorded: Plant height, fresh weight, dry weight, leaves number/plant, fruit number/plant and fruit weight/plant. Result: using treated wastewater significantly increased production parameters, the highest production was obtained with treated wastewater supplied with 10 ppm N-P-K fertilizer (81.5 cm/plant higher in vetch and 112.5 cm/plant in alfalfa for plant height). When treated wastewater was used alone compared to fresh water higher fresh weights (62.8 g/plant) (82.33 g/plant (132 and 87.17 g/plant) for vetch and alfalfa were obtained respectively. Regarding the dry weight, irrigation with TWW produced 33.83 g/plant and 44.67 g/plant compared to 22 g/plant and 24.5 g/plant for plants irrigated with fresh water for vetch and alfalfa respectively. The results of soil analysis show an increase in soil content of nitrogen, phosphorus and potassium, as well as an increase in soil salinity and SAR values.
Extension spatio-temporelle du modèle chèvre INRA 2018 : 1. Application à l'alimentation en lots
Authors: SAUVANT Daniel , CHAPOUTOT Patrick , GIGER-REVERDIN Sylvie , BLUET Bertrand 
Published: March 2022

Cette publication présente une démarche visant à aider à la gestion alimentaire des lots de chèvres laitières en s'appuyant sur le modèle individuel « chèvre » proposé par INRAE (INRA, 2018). Cette démarche consiste à s'appuyer sur des paramètres de la gestion des lots, en particulier sur le choix de l'animal cible qui est nourri à son potentiel. Ce choix permet i) de prédire les potentiels de production de chaque animal du lot en fonction des productions moyennes observées pour le lot et de la qualité des fourrages distribués, et ii) de prédire les réponses du lot en matière d'ingestion de concentrés et de fourrages, ainsi que de production de lait et de bilan nutritionnel, Outre l'animal cible, la chèvre moyenne, représentative de l'ensemble du lot, et la chèvre faible productrice, utilisée comme sentinelle pour des rations à risque, sont systématiquement prises en compte. Les démarches proposées sont applicables à différents contextes d'élevage. En particulier, elles peuvent être mises en œuvre pour établir une ration a priori ou bien pour analyser une ration constatée sur le terrain.
Two-Staged Total Knee Arthroplasty in Juvenile Rheumatoid Arthritis with Severe Flexion Deformity
Authors: Vecham Ratnakar , Annapareddy Adarsh , Hippalgaonkar Kushal , Bhushan Chandra , Pawar Sawankumar , Reddy A.V. Gurava 
Published: March 2022

Case:
Juvenile rheumatoid arthritis (JRA) is associated with severe flexion contracture of the knees disabling a patient's ability to walk. Although various treatment modalities are available in the literature, we report a 2-stage procedure in a case of a 22-year-old female JRA patient with bilateral severe flexion deformity of the knee: stage 1—correction of the flexion contracture deformity by ring fixators and stage 2—total knee arthroplasty (TKA) for arthritis. To the best of our knowledge, no such approach has been reported previously.

Conclusions:
An Ilizarov ring fixator is a safe technique for gradual correction of a severe flexion deformity. Subsequent TKA can be performed as a standard procedure.
Bilateral Pseudoarthrodesis
Authors: Assi Hussein , Massé Vincent , Saint-Yves Hugo , Barry Janie , St-Louis Jean , Isler Marc 
Published: March 2022

Case:
We present a severe hemophilia A patient with high titers of inhibitors presenting stage IV knee arthropathy with functional bilateral arthrodesis. On presentation, his mode of ambulation was to project himself forward without the benefit of any significant ankle motion. Total knee arthroplasty was performed on both knees and allowed significant improvement in the range of motion of both knees from 5° to 100°.

Conclusion:
Although hemophilic patients with inhibitors can represent complex cases, successful outcomes can be achieved in a multidisciplinary team setting. However, we would recommend performing this type of surgery at an earlier stage when less extensive muscle and tendon release is required.
Pediatric Lyme Disease Presenting as a Ruptured Popliteal Cyst
Authors: Sager Alora F. , Carolan Patrick L. , Georgiadis Andrew G. , Laine Jennifer C. 
Published: March 2022

Cases:
This case report describes 3 pediatric patients presenting with acute calf or knee pain, calf swelling, and a ruptured popliteal cyst diagnosed by magnetic resonance imaging. Lyme disease was serologically confirmed in each case. In all instances, treatment was delayed because of atypical presentation. All patients responded favorably after antibiotic therapy.

Conclusion:
The differential diagnosis of Lyme disease should be considered in the context of children presenting with atraumatic unilateral calf pain and a ruptured popliteal cyst. Otherwise, this unusual presentation could delay diagnosis or result in unnecessary surgical intervention, particularly in pediatric patients.
The Impact of APOE and Smoking History on Cognitive Function in Older, Long-Term Breast Cancer Survivors
Authors: Ahles Tim , Orlow Irene , Schofield Elizabeth , Li Yuelin , Ryan Elizabeth , Root James C. , Patel Sunita K. , McNeal Katrazyna , Gaynor Alexandra , Tan Heidi , Katheria Vani , Vazquez Jessica , Corrales-Guerrero Sergio , Sadeghi Keimya , Traina Tiffany , Hurria Arti 
Published: March 2022

Abstract
Purpose
To determine whether older breast cancer survivors score lower on neuropsychological tests compared to matched non-cancer controls and to test the hypotheses that survivors who were APOE ε4 carriers would have the lowest cognitive performance, but that smoking history would decrease the negative effect of ε4 on cognition.
Methods
Female breast cancer survivors who had been diagnosed and treated at age 60 or older and were 5 – 15 year survivors (N=328) and age and education matched non-cancer controls (N=160) were assessed at enrollment and at 8, 16 and 24 month follow ups with standard neuropsychological and psychological assessments. Blood for APOE genotyping was collected and smoking history was assessed at enrollment. Participants were purposely recruited so that approximately 50% had a history of treatment with chemotherapy or no chemotherapy and approximately 50% had a smoking history.
Results
After adjusting for age, cognitive reserve, depression and fatigue, breast cancer survivors scored significantly lower on all domains of cognitive function. A significant two-way interaction demonstrated that the negative effect of ε4 on cognitive performance was stronger among survivors. A significant three-way interaction supported the hypothesis that smoking history had a protective effect on cognitive function in ε4 carriers that was more pronounced in the controls than the survivors.
Conclusions
The results support the long-term cognitive impact of breast cancer diagnosis and treatments on older, disease-free survivors, particularly for ε4 carriers. The results also emphasize the importance of assessing smoking history when examining APOE and cognition and are an example of the complex interactions of age, genetics, health behaviors and disease history in determining cognitive function.
Effects of Tilt and Decentration of Visian Implantable Collamer Lens (ICL V4c) on Visual Quality: An Observational Study
Authors: Niu Lingling , Zhang Zhe , Miao Huamao , Zhao Jing , Li Meiyan , He Ji C , Yao Peijun , Zhou Xingtao 
Published: March 2022

Abstract
Objectives
To investigate the tilt and decentration effects of the Visian Implantable Collamer Lens (ICL V4c) on visual quality after implantation.
Methods
A total of 135 eyes from 69 patients who underwent ICL V4c implantation were included in this study. Evaluation of spherical equivalent, uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), and aberrations were performed 6-months postoperatively. The anterior segment parameters were collected using CASIA2 anterior segment-optical coherence tomography, and tilt and decentration of the ICL V4c were analyzed using MATLAB software. All patients received questionnaires to investigate the visual quality postoperatively.
Results
The safety and effectiveness were 1.18 ± 0.17 and 1.11 ± 0.18, respectively. No significant changes were observed regarding higher-order and spherical aberrations after the operation; however, coma and trefoil significantly increased compared to preoperative values. The average decentration was 0.21 ± 0.12 mm. The average tilt was 2.54 ± 1.00°. Horizontal, vertical, and total values of tilt and decentration were not significantly associated with postoperative CDVA, UDVA, and aberrations. The most common visual symptom was halo, and 97.04% of patients had a satisfaction score ≥ 8. The total or vertical tilt was significantly positively correlated with the frequency, severity, and bothersome scores from the questionnaires.
Conclusions
ICL V4c implantation can obtain high visual quality and patient satisfaction. Although the degree of tilt and decentration after ICL V4c implantation was small, a positive effect on subjective visual quality was observed.
Phylogenetic analysis of torquetenovirus in Romania: possible evidence of distinct geographical distribution
Authors: Cimponeriu Gheorghe Danut , Spandole-Dinu Sonia , Stoica Ileana , Apircioaie Oana , Gogianu Larisa , Berca Lavinia Mariana , Nica Silvia , Toma Mihai , Nica Remus 
Published: March 2022

Abstract
Torquetenovirus (TTV) is highly prevalent and little is known of its circulation in humans. We aimed to assess the geographical distribution and phylogeny of TTV from Romanians. A fragment of TTV UTR B was sequenced in samples from volunteers from all seven development regions of Romania. Additional sequences from Romanian dialyzed patients were added for phylogenetic analysis. More than 80% of Romanian sequences clustered with isolates assigned to TTV1 and TTV3 (former genogroup 1), while the rest clustered with TTV19-SANBAN and TTV22-svi-1 (former genogroup 3) and TTV-Hebei-1. Phylogenetic analysis showed segregation between isolates from North-East and West Romania providing possible evidence of distinct geographical distribution.
Beehive Sound: Can It Indicate The Response Of Honey Bees To Chemicals In Or Out Of The Beehive?
Authors: Yu Baizhong , Huang Xinqiu , Sharif Muhammad Zahid , Jiang Xueli , Di Nayan , Liu Fanglin 
Published: March 2022

Abstract
Honey bees (Apis spp.) are widely used as biological indicators of environmental changes. Recently, bees have been explored by researchers to monitor air contamination by listening to their hive sound. However, no study has determined whether beehive sound reflects the responses of bees to in-hive or out-hive chemicals. In this study, we conducted a feeding experiment to address this. First, we fed colonies with pure syrup (PS), syrup containing acetone (SA) or syrup containing ethyl acetate (SE) to collect beehive sound to establish multiple classifications using machine learning (ML) models. Then, we orderly fed colonies with PS, followed by SE, SA and PS. Next, we fed colonies in PS, SA, SE and PS order. Eventually, we evaluated the recall and precision of the model in detecting each syrup type. The result built on orderly feeding had a recall of 99%, 80%, 30%, 53% in detecting PS, SE, SA, PS, respectively. In the reverse feeding experiment, the ML model has a recall of 99%, 89%, 37% and 44% in detecting PS, SA, SE, and PS, respectively. Because the collected syrup in the two orderly feeding sessions was not removed from the frames during the experiment, the results indicate that beehive sound responds to chemicals in or out of the beehive.
Vaccinia Virus H7-Protein Organizes the Viral Scaffold Protein-Essential for Membrane and Virus Assembly
Authors: Tonnemacher Susanne , Folly-Klan Marcia , Gazi Anastasia , Schäfer Simon , Penard Esthel , Eberle Regina , Kunz Renate , Walther Paul , Locker Jacomine Krijnse 
Published: March 2022

Abstract
Viruses of the giant virus family are characterized by a structurally conserved scaffold-capsid protein that shapes the icosahedral virion. The vaccinia virus (VACV) scaffold protein D13, however, transiently shapes the newly assembled viral membrane in to a sphere and is absent from the mature brick-shaped virion. In infected cells D13, a 62kDa polypeptide, forms trimers that arrange in hexamers and a honey-comb like lattice. Membrane association of the D13-lattice may be mediated by A17, an abundant 21kDa viral membrane protein. Whether membrane binding mediates the formation of the honey-comb lattice or if other factors are involved, remains elusive. Here we show that H7, a 17kDa protein conserved among poxviruses, mediates proper formation of D13-hexamers, and hence the honey comb lattice and spherical immature virus. Without H7 synthesis D13 trimers organize into a regular network, composed of short D13 tubes of discrete length that are tightly associated with the endoplasmic reticulum (ER). The data show an unexpected role for H7 in D13 organization and imply that formation of the honey-comb, hexagonal, lattice is essential for VACV membrane assembly and production of infectious progeny. The data are discussed with respect to scaffold proteins of other giant viruses.
Reducing Contrast-Agent Volume And Radiation Dose In CT With 90­Kvp Tube Voltage, High Tube Current Modulation, And Advanced Iteration Algorithm
Authors: Park Min Su , Ha Hong Il , Ahn Jhii-Hyun , Lee Sang Min , Lee In Jae 
Published: March 2022

Abstract
The purpose of this study was to evaluate the image quality and the radiation dose in pancreatic dynamic CT with 90­kVp tube voltage and reduction of the standard amount of contrast agent, compared with 100­kVp PDCT. 51 patients who underwent both CT protocols were included in this retrospective study. One reviewer measured the attenuation values of the abdominal organs and image noise for objective image quality analysis. Two radiologists evaluated the five categories of image qualities for subjective image quality analysis. As a result, the total amount of the contrast agent, the radiation dose, and image noise decreased in the low-kVp group, by 24.4%, 31.7%, and 20.6%, respectively (p < 0.001). The intraobserver and interobserver agreements were moderate to substantial (k = 0.4 − 0.8). The SNR and CNR of the almost organs in the low-kVp group were significantly higher (p < 0.001). Except for lesion conspicuity, both reviewers judged that the subjective image quality of the low-kVp group was better (p < 0.001). With 90­kVp tube voltage, reduced contrast agent volume with advanced iteration and high tube current modulation achieved radiation dose reduction of 31.7%, as well as better image quality and diagnostic confidence.