bluuebunny/crossref_metadata_2025_split huggingface dataset:
SKI-INTERACTING PROTEIN interacts with SHOOT MERISTEMLESS to regulate shoot apical meristem formation
Authors: Li Ruiqi , Wei Zhifeng , Li Yan , Shang Xudong , Cao Ying , Duan Liusheng , Ma Ligeng 
Published: May 2022

AbstractThe shoot apical meristem (SAM), which is formed during embryogenesis, generates leaves, stems, and floral organs during the plant life cycle. SAM development is controlled by SHOOT MERISTEMLESS (STM), a conserved Class I KNOX transcription factor that interacts with another subclass homeodomain protein, BELL, to form a heterodimer, which regulates gene expression at the transcriptional level in Arabidopsis (Arabidopsis thaliana). Meanwhile, SKI-INTERACTING PROTEIN (SKIP), a conserved protein in eukaryotes, works as both a splicing factor and as a transcriptional regulator in plants to control gene expression at the transcriptional and posttranscriptional levels by interacting with distinct partners. Here, we show that, similar to plants with a loss of function of STM, a loss of function of SKIP or the specific knockout of SKIP in the SAM region resulted in failed SAM development and the inability of the mutants to complete their life cycle. In comparison, Arabidopsis mutants that expressed SKIP specifically in the SAM region formed a normal SAM and were able to generate a shoot system, including leaves and floral organs. Further analysis confirmed that SKIP interacts with STM in planta and that SKIP and STM regulate the expression of a similar set of genes by binding to their promoters. In addition, STM also interacts with EARLY FLOWERING 7 (ELF7), a component of Polymerase-Associated Factor 1 complex, and mutation in ELF7 exhibits similar SAM defects to that of STM and SKIP. This work identifies a component of the STM transcriptional complex and reveals the mechanism underlying SKIP-mediated SAM formation in Arabidopsis.
High-energy-level metabolism and transport occur at the transition from closed to open flowers
Authors: Borghi Monica , Perez de Souza Leonardo , Tohge Takayuki , Mi Jianing , Melandri Giovanni , Proost Sebastian , Martins Marina C M , Al-Babili Salim , Bouwmeester Harro J , Fernie Alisdair R 
Published: May 2022

Abstract
During the maturation phase of flower development, the onset of anthesis visibly marks the transition from buds to open flowers, during which petals stretch out, nectar secretion commences, and pollination occurs. Analysis of the metabolic changes occurring during this developmental transition has primarily focused on specific classes of metabolites, such as pigments and scent emission, and far less on the whole network of primary and secondary metabolites. To investigate the metabolic changes occurring at anthesis, we performed multi-platform metabolomics alongside RNA sequencing in individual florets harvested from the main inflorescence of Arabidopsis (Arabidopsis thaliana) ecotype Col-0. To trace metabolic fluxes at the level of the whole inflorescence and individual florets, we further integrated these studies with radiolabeled experiments. These extensive analyses revealed high-energy-level metabolism and transport of carbohydrates and amino acids, supporting intense metabolic rearrangements occurring at the time of this floral transition. These comprehensive data are discussed in the context of our current understanding of the metabolic shifts underlying flower opening. We envision that this analysis will facilitate the introgression of floral metabolic traits promoting pollination in crop species for which a comprehensive knowledge of flower metabolism is still limited.
Meeting the challenge of health system transformation in European countries
Authors: Hunter David J , Bengoa Rafael 
Published: May 2022

Abstract
Transforming health systems is a complex, messy business with no quick or simple solutions. Countries struggle to make it happen with policy failure often the result. Reporting on a World Health Organization Europe project aimed at understanding how health system transformation can succeed, the paper draws on three European country case studies to offer insights and lessons for policy-makers elsewhere engaged in similar efforts. Critical to success in implementation is the adoption of a receptive context for change. Building on the policy capacity literature, it emphasizes the importance of environmental pressure, the quality and coherence of policy, leadership style, supportive organizational culture, and managerial-health profession relations.
Boswell’s Self-hatred
Authors: Leonard-Roy Thomas 
Published: May 2022

Abstract
While scholars often mention the influence of James Boswell’s strict Presbyterian upbringing on his emotional life, his voluminous journals are typically approached in secular psychological terms This essay puts the journals in conversation with Calvinist-Christian theology and reads Boswell’s feelings, specifically his self-directed hateful ones, as religious in nature. Beginning with the vexed issue of hatred in Calvin and later seventeenth- and eighteenth-century religious writers, who exhort the believer to hate his sin while acknowledging the dangers posed by hatred, the essay then turns to Boswell’s religious attitudes and feelings. Boswell’s melancholy, shame after a night of drunkenness or prostitution, and statements such as ‘I despised myself’ or ‘my wretched existence’ demonstrate a mode of self-hatred that is distinctly Calvinist. ‘Correct’ Calvinist self-hatred—hating your sin and inclination to sinfulness—should provoke love of God, yet Boswell struggles to find any consolation in his hateful feelings. The journals prove to be powerful examples of the Calvinist degradation of the self, as well as important texts in the culture of the ‘long Reformation’.
Strong response after fourth dose of mRNA COVID-19 vaccine in autoimmune rheumatic diseases patients with poor response to inactivated vaccine
Authors: Aikawa Nadia E , Kupa Leonard V K , Silva Clovis A , Saad Carla G S , Pasoto Sandra G , Yuki Emily F N , Fusco Solange R G , Shinjo Samuel K , Andrade Danieli C O , Sampaio-Barros Percival D , Pereira Rosa M R , Chasin Anna C S , Shimabuco Andrea Y , Luppino-Assad Ana P , Leon Elaine P , Lopes Marta H , Antonangelo Leila , Medeiros-Ribeiro Ana C , Bonfa Eloisa 
Published: May 2022

Abstract

Objectives
To assess immunogenicity of a heterologous fourth dose of an mRNA (BNT162b2) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in autoimmune rheumatic diseases (ARD) patients with poor/non-response to inactivated vaccine (Sinovac-CoronaVac).

Methods
A total of 164 ARD patients who were coronavirus disease 2019 (COVID-19) poor/non-responders (negative anti-SARS-CoV-2 S1/S2 IgG and/or neutralizing antibodies—NAb) to the third dose of Sinovac-CoronaVac received an additional heterologous dose of mRNA (BNT162b2) 3 months after last dose. IgG and NAb were evaluated before and after the fourth dose.

Results
Significant increases were observed after the fourth dose in IgG (66.4 vs 95.1%, P &lt; 0.001), NAb positivity (5.5 vs 83.5%, P &lt; 0.001) and geometric mean titre (29.5 vs 215.8 AU/ml, P &lt; 0.001), and 28 (17.1%) remained poor/non-responders. Patients with negative IgG after a fourth dose were more frequently under rituximab (P = 0.001). Negative NAb was associated with older age (P = 0.015), RA (P = 0.002), SSc (P = 0.026), LEF (P = 0.016) and rituximab use (P = 0.007). In multiple logistic regression analysis, prednisone dose ≥7.5 mg/day (OR = 0.34; P = 0.047), LEF (OR = 0.32, P = 0.036) and rituximab use (OR = 0.19, P = 0.022) were independently associated with negative NAb after the fourth vaccine dose.

Conclusions
This is the largest study to provide evidence of a remarkable humoral response after the fourth dose of heterologous mRNA SARS-CoV-2 vaccination in ARD patients with poor/non-response to the third dose of an inactivated vaccine. We further identified that treatment, particularly rituximab and prednisone, impaired antibody response to this additional dose.

Trial registration
ClinicalTrials.gov, https://clinicaltrials.gov, CoronavRheum #NCT04754698.
A new tool supporting the diagnosis of childhood-onset Behçet’s disease: venous wall thickness
Authors: Atalay Erdal , Oguz Berna , Sener Seher , Ozcan H Nursun , Sag Erdal , Kaya Akca Ummusen , Kasap Cuceoglu Muserref , Balik Zeynep , Karakaya Jale , Karadag Omer , Basaran Ozge , Batu Ezgi Deniz , Bilginer Yelda , Ozen Seza 
Published: May 2022

AbstractObjectivesThe lower extremity venous wall thickness (VWT) of Behçet’s disease (BD) patients was reported to be significantly increased in adults, suggesting its use for the support of BD diagnosis. This prospective study aimed to investigate the lower extremity VWT in childhood-onset definite and incomplete BD patients and compare it to healthy age-matched controls.MethodsPaediatric patients classified with BD according to the 2015 international paediatric BD criteria in our centre were included in the study. Intima-media thickness of the lower extremity veins to evaluate VWT was measured by ultrasonography, including common femoral vein (CFV), femoral vein (FV), vena saphena magna, vena saphena parva and popliteal vein (PV).ResultsIn this cross-sectional study, VWT was measured in 35 patients (63% male) and 27 healthy controls (55% male). Thirteen (37%) of 35 patients met the criteria for the diagnosis of BD. The remaining 22 (63%) had incomplete BD and met two criteria. The median VWT values of both definite and incomplete BD patients were significantly higher than the control group in all veins on both sides. Regarding the best cut-off values of VWT for all lower extremity veins, the sensitivity rates were between 63% and 86%, while specificity rates were between 71% and 100%.ConclusionIncreased VWT was present not only in BD patients with vascular involvement but also in those without. We suggest that VWT may be a new criterion in supporting the diagnosis of childhood BD both in definite and incomplete BD patients.
Nailfold capillaroscopy: a survey of current UK practice and ‘next steps’ to increase uptake among rheumatologists
Authors: Eden Martin , Wilkinson Sarah , Murray Andrea , Bharathi Praveen Gurunath , Vail Andy , Taylor Chris J , Payne Katherine , Herrick Ariane L 
Published: May 2022

Abstract

Objectives
To identify barriers to the use of nailfold capillaroscopy as a diagnostic tool for patients presenting with Raynaud’s phenomenon in UK rheumatology centres and to obtain rheumatologists’ views on a proposed internet-based standardized system for clinical reporting of nailfold capillaroscopy images.

Methods
An online survey was developed using expert opinion from clinicians, scientists and health service researchers. The survey was piloted and sent to UK-based rheumatologists using established electronic mailing lists between October 2020 and March 2021. Survey data were analysed using descriptive statistics.

Results
A total of 104 rheumatologists representing rheumatology centres across the UK responded to the survey. Wide variations in terms of workloads and practices were described. Thirty-four (33%) respondents reported using nailfold capillaroscopy only at their own centre, 33 (32%) referred to other centres, 9 (9%) did both and 28 (27%) did not use capillaroscopy at all. Of the 43 respondents using capillaroscopy on site, 25 (58%) used either a dermatoscope or universal serial bus microscope and 9 (21%) used videocapillaroscopy. Among the 61 respondents not undertaking capillaroscopy on site, barriers included lack of equipment (85%), lack of experience in acquiring images (69%) and lack of expertise in interpreting images (67%). Sixty-six respondents (63%) expressed interest in an internet-based, standardized automated system for reporting images.

Conclusion
Most UK rheumatologists currently do not perform nailfold capillaroscopy on site. An internet-based nailfold capillaroscopy system for use with low-cost microscopes as well as with videocapillaroscopy could help increase uptake of capillaroscopy and thereby facilitate early diagnosis of SSc across the UK.
2021 NATIONAL DIAGNOSTIC REFERENCE LEVELS FOR PAEDIATRIC COMPUTED TOMOGRAPHY IN EGYPT
Authors: Abdou Sayed E , Salama Dina H , Ahmad Khaled A , Sallam Abdelsattar M , El-Sayed El-Sayed Mahmoud , Talaat M S , El-Farrash Rania A , Vassileva J 
Published: May 2022

Abstract
OBJECTIVE. To establish national diagnostic reference levels (NDRLs) for most common paediatric computed tomography (CT) examinations in Egypt. METHODS: This was a prospective study that included all dedicated paediatric imaging centers in Egypt. Data from 1680 individual paediatric patients undergoing CT scanning of the head, chest and abdomen–pelvis were collected. Computed tomography dose indices were recorded, data were analysed and compared with the internationally published paediatric DRLs in14 countries. RESULTS: The Egyptian NDRLs of the CTDIvol (mGy) for head, chest and abdomen–pelvis scans among four paediatric age groups were found to be (23, 27, 28, 32, 4, 5, 6, 8, 5, 6, 7, 9) mGy, respectively; and the corresponding NDRLs of the DLP (mGycm) for head, chest and abdomen–pelvis scans were found to be (345, 428, 499, 637, 67, 85, 145, 215, 97, 135, 240, 320) mGycm, respectively. There were variations in the radiation doses between CT centers and identical scanners indicating the need for dose optimization. The NDRLs of the CTDIvol (mGy) and the DLP (mGycm) values were similar to or lower than international DRLs. CONCLUSION: This study summarizes the results of the first Egyptian Computed Tomography survey that provides national diagnostic reference levels for paediatric patients in Egypt. Despite the reasonable NDRLs values, the study depicted certain pros and cons concerning CT practice, and identified some problems that hinder the process of optimization as well as justification in children.
0019 Drowsy Driving in a Community Sample: Associations with Sleep Duration, Insomnia Severity, Daytime Sleepiness, and Fatigue
Authors: Hamze Mohi , Kennedy Kathryn , Hale Lauren , Branas Charles , Killgore William , Wills Chloe , Grandner Michael 
Published: May 2022

Abstract

Introduction
Drowsy driving is an important public health concern. Yet, real-world data on drowsy driving patterns remains relatively scarce. The present study aimed to investigate reports of drowsy driving in a general community sample and whether these are associated with daytime and nighttime sleep health risk factors.

Methods
Data were obtained through the Sleep and Health Activity, Diet, Environment, and Socialization (SHADES) study, which recruited N=1,007 working-age adults from the Philadelphia area. Drowsy driving was assessed with the item from the CDC BRFSS, “During the past 30 days, have you ever nodded off or fallen asleep, even just for a brief moment, while driving?” Responses were coded as “yes” or “no” (or “don’t drive,” which was excluded). Sleep-related factors included sleep duration (NHANES item, assessed in hours), insomnia (Insomnia Severity Index [ISI]), sleepiness (Epworth Sleepiness Scale [ESS]), fatigue (Fatigue Severity Scale [FSS]), and sleep medication use (PSQI item). Covariates included age, sex, education, income, race/ethnicity, employment, body mass index, and stress (Perceived Stress Scale).

Results
The sample consisted of N=738 adults, excluding those who did not drive in the past 30 days. After adjustment for covariates, each hour of sleep duration was associated with a 23% reduction in likelihood of drowsy driving (OR=0.77; 95%CI[0.66,0.89]; p&lt;0.0005). Likelihood of drowsy driving increased with each point on the ISI by 8% (OR=1.08; 95%CI[1.04,1.13]; p&lt;0.0005), with each point on the ESS by 19% (OR=1.19; 95%CI[1.13,1.26]; p&lt;0.0005), and with each point on the FSS by 4% (OR=1.04; 95%CI[1.02,1.06]; p&lt;0.0005). Sleep medication use was not associated with drowsy driving. In a post-hoc model that combined duration, insomnia, sleepiness, and fatigue, unique effects were seen for sleepiness (OR=1.17; 95%CI[1.10.1.23]; p&lt;0.0005) and sleep duration (OR=0.82; 95%CI[0.70,0.98]; p=0.026).

Conclusion
Drowsy driving in a community sample is associated with less sleep duration and more insomnia, sleepiness, and fatigue. These effects may overlap, though daytime sleepiness emerged as the most robust risk factor. The combined model showed that sleep duration also contributed variance that was otherwise unexplained by the other factors. Drowsy driving prevention efforts should focus on sufficient sleep and daytime sleepiness as screening and prevention targets.

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0051 The Role of Perceived Control in Buffering Against Poor Sleep in Essential Workers During COVID-19
Authors: Meyer Mia , Hamm Jeremy , Garcia Odalis , Tan Jaron , Delaney Rachel , Duggan Katherine 
Published: May 2022

Abstract

Introduction
The COVID-19 pandemic has impacted sleep, with some populations such as essential workers reporting insomnia and poor sleep health. Prior research has suggested (but not tested) that this worsening of sleep may be tied to a lack of control over one’s health or safety during the pandemic. This study tests this prediction and examines the role of perceived control as a protective factor against poor sleep in essential workers.

Methods
This study uses data from the NDSU National COVID Study, which has followed 301 nationally-representative American adults across four waves of data collection since April 2020. The current analysis includes data from wave 1 (April 2020) in 279 participants who had complete demographic, essential worker, perceived control (including domain general perceived control as well as health, COVID, work-specific control), and sleep health (RU SATED) data. Using t-tests and correlations, we hypothesized: (1) sleep health would be worse in essential workers compared with others; (2) perceived control would relate to better sleep health; and (3) perceived control would be a stronger predictor of sleep health in essential workers relative to others.

Results
There were no significant differences in sleep health between essential workers (N=44, M=8.27, SD=2.72) and others (N=235, M=8.46, SD=2.54; t=-0.44, p=.66). In the full sample, all indices of perceived control were significantly related to better sleep health (rs=.17-.31, ps&lt;.004). Associations were stronger in essential workers (N=44, rs=.30-.56, ps&lt;.05) than in others (N=235, rs=.13-.31, ps&lt;.04). In sensitivity analyses that excluded participants not working for pay (e.g., people who were unemployed, retired, or receiving disability) from the other category, moderation effects were stronger; only COVID-related perceived control was significantly related to sleep health (N=110; r=.24, p=.01) in non-essential workers.

Conclusion
This is the first study to demonstrate links between perceived control and sleep. Although sleep health was not significantly different between essential and non-essential workers, we found that perceived control was especially beneficial for essential workers’ sleep. Our results suggest interventions to improve perceived control, a modifiable psychosocial resource, might improve sleep health for essential workers.

Support (If Any)
L30 HL143741 to KAD from NHLBI; Research and Creative Activity Award to KAD and JMH from NDSU.
0065 Geographic Association Between Neighborhood Sleep Health and Child Opportunity Index: Data at the Census Tract Level
Authors: Phan Sydney , Gorovoy Suzanne , Begay Tommy , Valencia Dora , Hale Lauren , Robbins Rebecca , Killgore William , Wills Chloe , Grandner Michael 
Published: May 2022

Abstract

Introduction
Sleep health impacts the community in many ways. Regional sleep health may reflect other important indicators of health and well-being. Few studies have examined sleep health at the regional level, though.

Methods
Data on neighborhood sleep health values were obtained from the “500 Cities” data collected by the CDC that includes census tract and proportion of the population in that region that report values associated with health, as assessed with the Behavioral Risk Factor Surveillance System. Data include the population of each census tract as well as census-estimated proportion of the population in each census tract that report obtaining at least 7 hours of sleep. Additional variables included as covariates in analyses included the proportion with healthcare access, that were obese, had high blood pressure, had diabetes, and were smokers. The Child Opportunity Index (COI) is a publicly-available index (DiversityDataKids.org) reported at the census tract level. It provides indices for “Education,” “Health & Environment,” and “Social & Economic” domains, as well as a global score. The present analysis merged the 500 Cities data with the COI data, using census tract as the matching variable. Linear regression analyses examined COI global and subscale scores as outcome variable and proportion of the population obtaining 7 hours of sleep as the independent variable, unadjusted and adjusted for covariates. When data were merged, 27,130 census tracts were included.

Results
Sleep sufficiency was associated with global COI, such that for each additional percent of the population that obtains &gt;=7 hours of sleep, COI increases by 3.6 points (95%CI[3.57,3.64]; p&lt;0.0001); this was attenuated in adjusted analyses (B=1.58; 95%CI[1.53,1.63]; p&lt;0.0001). Each component of COI was related to sleep sufficiency, including education (B=3.06; 95%CI[1.19,1.33]; &lt;0.0001), health & environment (B=3.61; 95%CI[3.57,3.64]; p&lt;0.0001), and social & economic (B=2.23; 95%CI[2.19,2.28]; p&lt;0.0001). All associations were attenuated but significant in adjusted analyses.

Conclusion
Regional prevalence of insufficient sleep was linearly associated with Child Opportunity Index, which itself is an important predictor of a wide range of health and economic outcomes. Community sleep health interventions may have wide-ranging benefits.

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