bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multimodal Image Normalisation Tool (MINT) for the Adolescent Brain and Cognitive Development study: the MINT ABCD Atlas
Authors: Pecheva Diliana , Iversen John R , Palmer Clare E , Watts Richard , Jernigan Terry L , Hagler Donald J , Dale Anders M 
Published: August 2022

AbstractThe Adolescent Brain and Cognitive Development (ABCD) study aims to measure the trajectories of brain, cognitive, and emotional development. Cognitive and behavioural development during late childhood and adolescence have been associated with a myriad of microstructural and morphological alterations across the brain, as measured by magnetic resonance imaging (MRI). These associations may be strongly localised or spatially diffuse, therefore, it would be advantageous to analyse multimodal MRI data in concert, and across the whole brain. The ABCD study presents the unique challenge of integrating multimodal data from tens of thousands of scans at multiple timepoints, within a reasonable computation time. To address the need for a multimodal registration and atlas for the ABCD dataset, we present the synthesis of an ABCD atlas using the Multimodal Image Normalisation Tool (MINT). The MINT ABCD atlas was generated from baseline and two-year follow up imaging data using an iterative approach to synthesise a cohort-specific atlas from linear and nonlinear deformations of eleven channels of diffusion and structural MRI data. We evaluated the performance of MINT against two widely used methods and show that MINT achieves comparable alignment to current state-of-the-art multimodal registration, at a fraction of the computation time. To validate the use of the ABCD MINT atlas in whole brain, voxelwise analysis, we replicate and expand on previously published region-of-interest analysis between diffusion MRI-derived measures and body mass index (BMI). We also report novel association between BMI and brain morphology derived from the registration deformations. We present the ABCD MINT atlas as a publicly available resource to facilitate whole brain voxelwise analyses for the ABCD study.
Inflammatory bowel disease-associated gut commensals degrade components of the extracellular matrix
Authors: Porras Ana Maria , Zhou Hao , Shi Qiaojuan , Xiao Xieyue ,   JRI Live Cell Bank, Longman Randy , Brito Ilana Lauren 
Published: August 2022

ABSTRACTExtracellular matrix (ECM) remodeling has emerged as a key feature of inflammatory bowel disease (IBD), and ECM fragments have been proposed as markers of clinical disease severity. Recent studies report increased protease activity in the gut microbiota of IBD patients. Nonetheless, the relationship between gut microbiota and ECM remodeling has remained unexplored. We hypothesized that members of the human gut microbiome can degrade host ECM, and that bacteria-driven remodeling, in turn, can enhance colonic inflammation. Through a variety ofin vitroassays, we first confirmed that multiple bacterial species found in the human gut are capable of degrading specific ECM components. Clinical stool samples obtained from ulcerative colitis patients also exhibited higher levels of proteolytic activityin vitrocompared to those of their healthy counterparts. Furthermore, culture supernatants from bacteria species capable of degrading human ECM accelerated inflammation in a dextran sodium sulfate (DSS)-induced colitis. Finally, we identified several of the bacterial proteases and carbohydrate degrading enzymes (CAZymes) potentially responsible for ECM degradationin vitro. Some of these protease families and CAZymes were also found in increased abundance in a metagenomic cohort of IBD. These results demonstrate that some commensal bacteria in the gut are indeed capable of degrading components of human ECMin vitroand suggest this proteolytic activity may be involved in the progression of IBD. A better understanding of the relationship between nonpathogenic gut microbes, host ECM, and inflammation could be crucial to unravel some of the mechanisms underlying host-bacteria interactions in IBD and beyond.
Nutrition scores and MUAC of adult surgical orthopaedic inpatients at a teaching hospital in Lusaka province, Zambia
Authors: Miyoba Nixon , Ogada Irene 
Published: August 2022

AbstractBackgroundPoor nutrition status among hospitalised patients has been shown to increase length of hospital stay, as well as contribute to increased morbidity and mortality. The purpose of the study was to evaluate the nutrition status of adult surgical orthopaedic patients attending a teaching hospital in Zambia.MethodsThis study adopted a hospital-based cross-sectional study design to collect data from 98 adult patients aged 18 - 64 years. A structured questionnaire, the Subjective Global Assessment (SGA) tool, mid-upper arm circumference (MUAC) tape were used to collect data during the study period of three months in 2015.ResultsThe mean age of the patients was 36.4 plus or minus 9.44 years, while the mean length of hospital stay was 17.33 plus or minus 10.91 days. Nutrition-focused physical examination revealed that majority (89.8%) of the patients were of acceptable weight with no weight loss reported in 70.4% of the patients. Poor appetite was only reported by 10.2% of the patients. SGA findings suggest that most of the patients (79.6%) were well-nourished. The mean mid-upper arm circumference of the study participants during hospitalization was 25.09 plus or minus 2.85 cm. An association was found between length of hospital stay and mid-upper arm circumference of the patients (p<0.001).ConclusionSubjective Global Assessment has the potential to evaluate the nutrition status of surgical patients in resource-poor settings such as Zambia. However, the use of SGA should be supplemented by other tools such as MUAC which has the potential to screen for adult malnutrition in clinical settings with limited resources.
Genetic variation associated with relative resistance in teak (Tectona grandis L. f.) against the leaf skeletonizer, Eutectona machaeralis Walker
Authors: Vaishnav Vivek , Kulkarni Nitin , Ansari Shamim Akhtar , Rana Tikam Singh 
Published: August 2022

AbstractBackgroundPhotosynthesizing tissue of teak (Tectona grandis L. f.) foliage is damaged by a host-specific insect pest called leaf skeletonizer (Eutectona machaeralis Walker) that severely eclipses annual growth increment and carbon sequestration of natural populations and plantation of teak. Gene-assisted selection of relatively resistant teak clones may efficiently control the damage in the populations and plantations. The present investigation aimed to identify genetic variation associated with relative resistance in teak against the pest.MethodThe investigation was carried out on 106 teak plus tree clones assembled at the National Teak Germplasm Bank from the Indian meta-population of teak. Resistance data were obtained recording the ocular damage caused by the pest to teak accessions for four years. Genotyping of the teak accessions was performed with 21 co-dominant markers and marker-trait association mapping was performed confirming the genetic structure of the germplasm bank and linkage disequilibrium (LD) among the marker loci.ResultsThe sampled teak accessions exhibited a low albeit highly admixed genetic structure (FST=0.07) and low level of LD (16.66%) among loci, making them suitable for high-resolution association analysis. A significant correlation (p≤0.01, R2=0.67) was obtained between intra-specific heterozygosity and the relative resistance against the pest. A marker locus CCoAMT-1 representing the enzyme caffeoyl-CoA O-methyltransferase of phenylpropanoid pathway was also found significantly (p≤0.05) associated with the relative resistance against the pest explaining 6.6% of the phenotypic variation (R2=0.066) through positive effect (0.57) on the trait.ConclusionsThe present work exhibited a significant correlation of intra-specific heterozygosity with relative resistance in teak against a pest. It is the first report on teak identifying genetic markers associated with relative resistance against the pest. The marker can be applied for the selection of resistant planting stock for breeding and commercial plantation.Further investigation can be performed to understand the expression level polymorphism linked with the resistance applying next-generation sequencing approaches.
Identification of β2 microglobulin, the product of B2M gene, as a Host Factor for Vaccinia Virus Infection by Genome-Wide CRISPR genetic screens
Authors: Matía Alejandro , Lorenzo Maria M. , Romero-Estremera Yolimar C. , Sanchez-Puig Juana M. , Zaballos Angel , Blasco Rafael 
Published: August 2022

AbstractGenome-wide genetic screens are powerful tools to identify genes that act as host factors of viruses. We have applied this technique to the analyze the infection of HeLa cells by Vaccinia virus, in an attempt to find genes necessary for infection. Infection of cell populations harboring single gene inactivations resulted in no surviving cells, suggesting that no single gene knock-out was able to provide complete resistance to Vaccinia virus and thus allow cells to survive infection. In the absence of an absolute infection blockage, we explored if some gene inactivations could provide partial protection leading to a reduced probability of infection. Multiple experiments using modified screening procedures involving replication restricted viruses led to the identification of multiple genes whose inactivation potentially increase resistance to infection and therefore cell survival. As expected, significant gene hits were related to proteins known to act in virus entry, such as ITGB1 and AXL as well as genes belonging to their downstream related pathways. Additionally, we consistently found β2-microglobulin, encoded by the B2M gene, among the screening top hits, a novel finding that was further explored. Inactivation of B2M resulted in 54% and 91% reduced VV infection efficiency in HeLa and HAP1 cell lines respectively. In the absence of B2M, while virus binding to the cells was unaffected, virus internalization and early gene expression were significantly diminished. These results point to β2-microglobulin as a relevant factor in the Vaccinia virus entry process.Author summaryOrthopoxviruses, a genus belonging to the familyPoxviridae, include human pathogens like Variola virus, the causative agent of the now eradicated Smallpox, and Monkeypox virus that cause human outbreaks of zoonotic origin. Being the prototype Poxvirus,Vaccinia virushas been extensively used as the ideal model to study infection. For Poxviruses, both fluid phase endocytosis and direct fusion at the plasma membrane have been described as modes of entry. To date, only a few cellular factors have been identified in the vaccinia virus entry pathway. In this study, we report that blind genome-wide genetic screens allowed us to identify several cellular factors involved in Vaccinia Virus infection, of which many could be related to known factors in virus entry. In addition, we found that β2-microglobulin constitute a novel player for Poxvirus entry not related to previously described cellular pathways involved in the entry process. These findings add new information to the complex picture of Poxvirus entry and open the door to the discovery of new entry mechanisms used by Poxviruses.
Deterministic and probabilistic fate decisions co-exist in a single retinal lineage
Authors: Nerli Elisa , Kretzschmar Jenny , Bianucci Tommaso , Rocha-Martins Mauricio , Zechner Christoph , Norden Caren 
Published: August 2022

SummaryCorrect nervous system development depends on the timely differentiation of progenitor cells into neurons. While the output of progenitor differentiation is well investigated at the population and clonal level, the possibilities and constraints for fate decisions of specific progenitors over development are less explored. Particularly little is known about their variability and competence plasticity. To fill this gap, we here use long-term live imaging to follow the outcome of progenitor divisions in the zebrafish retina.We find that neurogenic Atoh7 expressing progenitors produce different neuronal types over development with time-dependent probabilities. Interestingly, deterministic and probabilistic fate decisions co-exist in the same lineage. While interference with the deterministic fate affects lineage progression, interference with fate probabilities of the stochastic lineage branch results in a broader range of fate possibilities than seen in controls. When tissue development is challenged, Atoh7 expressing progenitors can produce any neuronal type, arguing against the concept of fixed competence windows. Stochastic modelling of fate probabilities in challenged conditions revealed a simple gene regulatory network able to recapitulate the observed competence changes during development. Based on our results, we postulate that fate plasticity could be involved in robust retinal development, a concept possibly applicable to other tissues.
Role of human HSPE1 for OPA1 processing independent of HSPD1
Authors: Yeung Nelson , Murata Daisuke , Iijima Miho , Sesaki Hiromi 
Published: August 2022

AbstractThe mtHSP60/HSPD1-mtHSP10/HSPE1 system prevents protein misfolding and maintains proteostasis in the mitochondrial matrix. Altered activities of this chaperonin system have been implicated in human diseases, such as cancer and neurodegeneration. However, how defects in HSPD1 and HSPE1 affect mitochondrial structure and dynamics remains elusive. In the current study, we address this fundamental question in a human cell line, HEK293T. We found that the depletion of HSPD1 or HSPE1 results in fragmentation of mitochondria, suggesting a decrease in mitochondrial fusion. Supporting this notion, HSPE1 depletion led to proteolytic inactivation of OPA1, a dynamin-related GTPase that fuses the mitochondrial membrane. This OPA1 inactivation was mediated by a stress-activated metalloprotease, OMA1. In contrast, HSPD1 depletion did not induce OMA1 activation or OPA1 cleavage. These data suggest that HSPE1 controls mitochondrial morphology through a mechanism separate from its chaperonin activity.
Humoral responses against HDL particles are linked to lipoprotein traits, atherosclerosis occurrence, inflammation and pathogenic pathways during the earliest stages of arthritis
Authors: Rodríguez-Carrio Javier , Alperi-López Mercedes , López Patricia , Pérez-Álvarez Ángel I. , Robinson George A. , Alonso-Castro Sara , Amigó Núria , Atzeni Fabiola , Suárez Ana 
Published: August 2022

ABSTRACTObjectivechronic inflammation and immune dysregulation are crucial mechanisms for atherosclerosis in rheumatoid arthritis (RA). Recent evidence suggests a link via humoral responses against high-density lipoproteins (HDL). However, their specificity, clinical relevance and emergence along disease course are unknown, especially during the earliest phases of RA.MethodsIgG and IgM serum levels of antibodies against HDL (anti-HDL) and Apolipoprotein A1 (anti-ApoA1) were measured in 82 early RA patients, 14 arthralgia individuals and 96 controls. Established RA patients (n=42) were included for validation. Atherosclerosis and vascular stiffness were measured by Doppler-ultrasound. Lipoprotein content, particle numbers and size were measured by H-NMR. Cytokines were measured by immunoassays. A cardiometabolic-related protein panel was evaluated using high- throughput targeted proteomics.Resultsanti-HDL and anti-ApoA1 responses were increased in early RA compared to controls (both p<0.001) and were comparable to established disease. Only anti-ApoA1 antibodies were increased in arthralgia. IgG anti-HDL and anti-ApoA1 were associated with unfavourable lipoprotein traits in RA and arthralgia, respectively. A similar picture was observed for inflammatory mediators. No associations with clinical features or risk factors were found. IgG anti-HDL were independently associated with atherosclerosis occurrence in early RA, and outperformed patient stratification over conventional algorithms (mSCORE) and their anti-ApoA1 counterparts. Anti-HDL antibodies correlated with proteins involved in immune activation, remodelling, and lipid metabolism pathways in early RA.Conclusionhumoral responses against HDL particles are an early event along arthritis course, although quantitative and qualitative differences can be noticed among stages. These differences informed distinct capacities as biomarkers and underlying pathogenic circuits.
A robust scheme for numerical simulation of heat transfer in two-fluid flows with high volumetric heat capacity contrasts
Authors: Lu Min , Yang Zixuan , He Guowei 
Published: August 2022

Purpose
This paper aims to propose a new method for robust simulations of passive heat transfer in two-fluid flows with high volumetric heat capacity contrasts.

Design/methodology/approach
This paper implements a prediction–correction scheme to evolve the volumetric heat capacity. In the prediction substep, the volumetric heat capacity is evolved together with the temperature. The bounded downwind version of compressive interface capturing scheme for arbitrary meshes and central difference scheme are used for the spatial discretization of the advection and diffusion terms of the heat transfer equation, respectively. In the correction substep, the volumetric heat capacity is updated in accordance with the interface captured by using a coupled level-set and volume-of-fluid method to capture the interface dynamics precisely.

Findings
The proposed method is verified by simulating the advection of a hot droplet with high volumetric heat capacity, a stationary air–water tank with temperature variation between top and bottom walls and heat transfer during wave plunging at

Re=108. The test results show that the proposed method is practical and accurate for simulating two-fluid heat transfer problems, especially for those feature high volumetric heat capacity contrasts.

Originality/value
To ensure the numerical stability, this paper solves an additional conservative form of volumetric heat capacity equation along with the conservative form of temperature equation by using consistent spatial-discretization and temporal-integration schemes.
Guest service experience in eco-centric hotels: a content analysis
Authors: Olorunsola Victor Oluwafemi , Saydam Mehmet Bahri , Arasli Huseyin , Sulu Deniz 
Published: August 2022

PurposeSustainable tourism is becoming more popular all over the world. Eco-friendly (green) hotels are properties that are friendly to the environment and are becoming increasingly popular among green travellers. Electronic word-of-mouth is a technique of communicating with consumers in order to share their experiences, and it is a significant marketing tool for hotels. This paper aims to identify the main themes shared in online reviews by tourists visiting eco-friendly hotels, and which of these themes were associated with satisfaction and dissatisfaction ratings.Design/methodology/approachThe research used qualitative content analyses to analyse 1,202 user-generated content of the top 10 hotels in UK shared by guests on an online platform.FindingsThe analyses revealed nine themes in descriptions of airline travel experiences. These are “hotel amenities”, “services”, “location”, “staff”, “eco” (eco-friendly activities), “value” and “recommend/revisit” (intentions). Negative comments are associated with the “bathroom”, “mattress”, “water”, “bed”, “price”, “shower”, “Wi-Fi” and “restaurant” concepts.Originality/valueThis study differs from previous research in which it aims to address a void in the literature on the shortcomings of research focused on finding the dominant themes expressed in online reviews by tourists visiting eco-friendly hotels, and it does so using data mining approach.
Understanding the impact of social learning forms on environmentally sustainable consumption behavior among school children
Authors: Sharma Meenakshi 
Published: August 2022

PurposeThe learning environment is not a vacuum, but rather is rich in social contexts with innumerable points, interests, and beliefs. This takes one of the concepts of “social learning” which is not just a natural occurring phenomenon but also a way of organizing learning and communities of learners. Social Learning (SL) tends to refer to learning that takes place when divergent interests, norms and values of reality meet in an environment that is conducive to learning. This paper helps to determine the impact of SL forms on environmentally sustainable consumption behavior among school children.Design/methodology/approachAfter conducting an experimental study among school children, data were analyzed using ANOVA. The total sample size was 760.FindingsSL forms as an intervention influence environmentally sustainable consumption behavior (ESCB) among school children. The findings of this paper suggest that SL techniques such as visuals had been able to positively impact the constructs like knowledge, comprehension, and application. This was very much supported by observations of children, interactions with them and the upfront support provided by school and teachers.Research limitations/implicationsBased on the result, the paper identified that although SL forms influence the ESCB, there is a need to identify the most effective SL forms having impact on ESCB.Practical implicationsThis study will help marketers to understand that what type of learning forms can be used to enhance the environmentally sustainable consumption behavior among children.Originality/valueThis paper is completely an original work carried out by the author.
Serving the community while balancing multiple responsibilities – experiences of working as a paid part-time firefighter
Authors: Lantz Emelie , Nilsson Bengt , Elmqvist Carina , Fridlund Bengt , Svensson Anders 
Published: August 2022

PurposeThe purpose of this study was to describe experiences of working as a paid part-time firefighter (PTF) in Swedish rural areas.Design/methodology/approachAn inductive explorative design was used, based on interviews with 18 paid PTFs in Sweden. Data were analyzed using qualitative latent content analysis.FindingsThree themes emerged from the interviews and describe paid PTFs’ experiences. The findings provide insights into how firefighters share a strong commitment, how support plays a crucial role, and how training and call-outs contribute to their experiences. Paid PTFs’ experiences are nuanced, ranging from personal limitations and challenges to satisfaction and the contrast with ordinary life.Practical implicationsThe implications for fire and rescue service organizations are that they can encourage firefighters’ commitment and pride, as well as the commitment and support of their families and main employers. Further, highlighting the importance of support and facilitating flexibility when on call is crucial. Finally, acknowledging and promoting personal development and facilitating an inclusive culture are important factors for both motivation and satisfaction.Originality/valuePaid PTFs are under-represented in the literature, despite the reliance on them in Sweden, and this study begins to address the knowledge gap. To improve retention, it is vital to understand paid PTFs work situation: what motivates them, what barriers they face, and how those challenges influence their experiences.