bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pushing the Natural Frontier: Progress on the Integration of Biomaterial Cues toward Combinatorial Biofabrication and Tissue Engineering
Authors: Guimarães Carlos F. , Marques Alexandra P. , Reis Rui L. 
Published: April 2022

AbstractThe engineering of fully functional, biological‐like tissues requires biomaterials to direct cellular events to a near‐native, 3D niche extent. Natural biomaterials are generally seen as a safe option for cell support, but their biocompatibility and biodegradability can be just as limited as their bioactive/biomimetic performance. Furthermore, integrating different biomaterial cues and their final impact on cellular behavior is a complex equation where the outcome might be very different from the sum of individual parts. This review critically analyses recent progress on biomaterial‐induced cellular responses, from simple adhesion to more complex stem cell differentiation, looking at the ever‐growing possibilities of natural materials modification. Starting with a discussion on native material formulation and the inclusion of cell‐instructive cues, the roles of shape and mechanical stimuli, the susceptibility to cellular remodeling, and the often‐overlooked impact of cellular density and cell–cell interactions within constructs, are delved into. Along the way, synergistic and antagonistic combinations reported in vitro and in vivo are singled out, identifying needs and current lessons on the development of natural biomaterial libraries to solve the cell–material puzzle efficiently. This review brings together knowledge from different fields envisioning next‐generation, combinatorial biomaterial development toward complex tissue engineering.
Coupling Localized Laser Writing and Nonlocal Recrystallization in Perovskite Crystals for Reversible Multidimensional Optical Encryption
Authors: Li Mingjia , Yang Dandan , Huang Xiongjian , Zhang Hao , Zhao Yifei , Yin Bozhao , Pan Qiwen , Kang Juan , Zheng Nan , Liu Xiaofeng , Qiu Jianrong , Yang Zhongmin , Dong Guoping 
Published: April 2022

AbstractThe ability to generate and manipulate photoluminescence (PL) with high spatial resolution has been of primary importance for applications in micro‐optoelectronics, while the emerging metal halide perovskites offer novel material platforms where diverse photonic functionalities and fine structuring are constantly explored. Herein, micro‐PL patterns consisting of highly luminescent CsPbBr3 nanocrystals (NCs) in nonluminescent perovskite crystals are directly fabricated by focused femtosecond laser irradiation. Further modulation with a moisture field leads to the selective dissolution of the laser‐destabilized perovskite structures as revealed by density functional theory simulations, thus allowing for facile control of the reversible PL from the recrystallization of moisture‐induced CsPbBr3 NCs. By leveraging the coupled laser writing and moisture modulation, multimodal information encryption is realized by reversible encryption–reading and repeatable erasing–refreshing. This optical storage mechanism is also extended to 3D and 4D by realizing spatially and temporally resolved optical encryption. The coupled multifield modulation on perovskite crystals can enable potential applications in optical storage and encryption, and offer a novel solution for the creation and manipulation of localized PL structures with high temporal and spatial resolutions.
Fully Textile X‐Ray Detectors Based on Fabric‐Embedded Perovskite Crystals
Authors: Possanzini Luca , Basiricò Laura , Ciavatti Andrea , Tessarolo Marta , Fraboni Beatrice 
Published: April 2022

AbstractThe interest and thrust for wearable ionizing radiation dosimeters are rapidly growing, stimulated by a large number of different applications impacting on humankind, spanning from medicine to civil security and space missions. Lead halide perovskites are considered one of the most promising classes of novel materials for X‐ray detectors due to their superior electronic and detection performance coupled with compatibility with solution‐based printing processes, allowing fabrication onto flexible substrates. It is reported on fully textile perovskite‐based direct X‐ray detectors, where the photoactive layer is constituted by a silk‐satin fabric functionalized with methylammonium lead bromide perovskite crystals embedded in the textile. The reliability of the proposed fabrication process, based on simple and low‐tech deposition techniques adaptable to industrial printing technologies for textiles, is assessed by realizing different detector's architectures that exhibit comparable detection performances. Sensitivity values up to (12.2 ± 0.6) µC Gy−1 cm−2 and a limit of detection down to 3 μGy s−1 are achieved, and low bias operation (down to 1 V) is demonstrated, validating wearable applications. Further, fully textile pixelated matrix X‐ray sensors are implemented and tested, providing the proof of principle for large‐area scalability.
Interface Engineering of Bi‐Fluorescence Molecules for High‐Performance Data Encryption and Ultralow UV‐Light Detection
Authors: Wang Jian‐Xin , Gutiérrez‐Arzaluz Luis , Yin Jun , Maity Partha , Zhou Yang , Chen Cailing , Han Yu , Bakr Osman M. , Eddaoudi Mohamed , Mohammed Omar F. 
Published: April 2022

AbstractIt is extremely difficult if not impossible to effectively and precisely regulate the luminescence of organic chromophores from different electronic excited states through external stimuli for use in light‐conversion devices. This is mainly due to the difficulty in breaking Kasha's rule by large energy separation and stabilization of different emissive electronic excited states. Here, the authors address this great challenge in a single experiment by expanding the utility of a monounsaturated omega‐9 fatty acid (oleic acid) capped with organic chromophores as a new and efficient luminescent regulator. More specifically, the authors have successfully promoted the use of oleic acid as an efficient and reversible switch that can precisely regulate chromophore luminescence. These time‐resolved absorption and luminescence experiments, along with density functional theory calculations have clearly demonstrated that ultrafast electron transfer from oleic acid to the difluoroboron β‐diketonate (DFBK) chromophores efficiently blocks the intramolecular charge transfer process of DFBK chromophores, and activates the locally excited state luminescence, leading to different emission colors from different electronic excited states for ultralow UV‐light detection and high‐performance data encryption.
Regulation of Irradiation‐Dependent Long‐Lived Room Temperature Phosphorescence by Controlling Molecular Structures of Chromophores and Matrix
Authors: Wang Zhonghao , Qu Lunjun , Gao Liang , Zheng Xian , Zheng Yan , Zhu Yinyin , Xia Jie , Zhang Yongfeng , Wang Chang , Li Youbing , Yang Chaolong 
Published: April 2022

AbstractLong‐lived room temperature phosphorescent (LRTP) materials have attracted widespread attention due to their unique luminescence phenomenon and application prospects in the fields of information encryption and bioimaging. However, achieving intelligent response LRTP from amorphous polymers under atmospheric conditions is fascinating but challenging. Here, a series of irradiation‐dependent LRTP systems with ultralong phosphorescent lifetime and high quantum yields are fabricated by a consuming triplet oxygen strategy, and the LRTP performance exhibits obvious chromophore structure and polymeric molecular weight dependence. By extending the UV irradiation time, the phosphorescence lifetime can be increased by hundreds of times, which can exceed 2 s. Experimental results prove that the molecular weight of the polymer matrix, the doping concentration, and the structure of the chromophore have a crucial influence on the stimulus‐response speed and phosphorescence properties of the materials. This kind of intelligent and irradiation‐dependent LRTP material has excellent application prospects in the field of multi‐level information encryption. This work will effectively promote the development of stimulus‐responsive LRTP materials under atmospheric conditions.
Glacial vicariance and oceanic circulation shape population structure of the coastal legume Derris trifoliata in the Indo‐West Pacific
Authors: Banerjee Achyut Kumar , Feng Hui , Guo Wuxia , Harms Nathan E. , Xie Hongxian , Liang Xinru , Xing Fen , Lin Yuting , Shao Huiyu , Guo Zixiao , Ng Wei Lun , Huang Yelin 
Published: April 2022

AbstractPremiseThe phylogeography of coastal plant species is shaped by contemporary and historical biogeographic processes. In this study, we aim to decipher the phylogeography of Derris trifoliata, a woody legume of relatively recent origin and wide distribution, in coastal areas in the Indo‐West Pacific (IWP) region.MethodsGenetic diversity and population structure were assessed by analyzing six nuclear and three chloroplast DNA sequences from 30 populations across the species’ range. Phylogeography was inferred by estimating gene flow, divergence time, historical population size changes, and historical habitat suitability using paleoclimatic niche modeling.ResultsHigh genetic diversity was observed at the species level. The populations of three oceanic regions included in this study (i.e., Indian Ocean, South China Sea, and Pacific Ocean) formed distinct clades and likely diverged during the late Pleistocene. Potential barriers to gene flow were identified, including the Sunda and Sahul shelves, geographic distance, and current patterns of oceanic circulation. Analysis of changes in population size supported the bottleneck model, which was strengthened by estimates of habitat suitability across paleoclimatic conditions.ConclusionsThe once widespread distribution of D. trifoliata was fragmented by changes in climatic suitability and biogeographic barriers that arose following sea‐level changes during the Pleistocene. In addition, contemporary patterns of oceanic circulation and geographic distance between populations appear to maintain genetic differentiation across its distribution in the IWP.
Stereoselective Synthesis of Allenyl Alcohols by Cobalt(III)‐Catalyzed Sequential C−H Bond Addition to 1,3‐Enynes and Aldehydes
Authors: Xu Chaofan , Tassone Joseph P. , Mercado Brandon Q. , Ellman Jonathan A. 
Published: April 2022

AbstractAn efficient and stereoselective CoIII‐catalyzed sequential C−H bond addition to 1,3‐enynes and aldehydes is disclosed. This transformation represents the first example of sequential C−H bond additions to 1,3‐enynes and a second coupling partner and provides the first example of preparing allenes by C−H bond addition to 1,3‐enynes. A wide range of aldehydes, C−H bond substrates and 1,3‐enynes with large substituents on the alkynes are effective substrates. The allenyl alcohol products can be further converted to dihydrofurans with high stereoselectivity either in situ or under Ag‐mediated cyclization conditions. The allenyl silyl group can also be transferred to the adjacent alcohol by a Brook rearrangement. Moreover, a mechanism for the transformation is proposed supported by X‐ray structural characterization of a cobaltacycle intermediate.
A Single‐Molecule Insight into the Ionic Strength‐dependent, Cationic Peptide Nucleic Acids‐Oligonucleotides Interactions
Authors: Asandei Alina , Mereuta Loredana , Bucataru Ioana C. , Park Yoonkyung , Luchian Tudor 
Published: April 2022

AbstractTo alleviate solubility‐related shortcomings associated with the use of neutral peptide nucleic acids (PNA), a powerful strategy is incorporate various charged sidechains onto the PNA structure. Here we employ a single‐molecule technique and prove that the ionic current blockade signature of free poly(Arg)‐PNAs and their corresponding duplexes with target ssDNAs interacting with a single α‐hemolysin (α‐HL) nanopore is highly ionic strength dependent, with high salt‐containing electrolytes facilitating both capture and isolation of such complexes. Our data illustrate the effect of low ionic strength in reducing the effective volume of free poly(Arg)‐PNAs and augmentation of their electrophoretic mobility while traversing the nanopore. We found that unlike in high salt electrolytes, the specific hybridization of cationic moiety‐containing PNAs with complementary negatively charged ssDNAs in a salt concentration as low as 0.5 M is dramatically impeded. We suggest a scenario in which reduced charge screening by counterions in low salt electrolytes enables non‐specific, electrostatic interactions with the anionic backbone of polynucleotides, thus reducing the ability of PNA‐DNA complementary association via hydrogen bonding patterns. We applied an experimental strategy with spatially‐separated poly(Arg)‐PNAs and ssDNAs, and present evidence at the single‐molecule level suggestive of the real‐time, long‐range interactions‐driven formation of poly(Arg)‐PNA‐DNA complexes, as individual strands entering the nanopore from opposite directions collide inside a nanocavity.
Rising Interest in the Development of Metal Complexes in Cancer Immunotherapy
Authors: Li Zhi‐Yuan , Shen Qing‐Hua , Mao Zong‐Wan , Tan Cai‐Ping 
Published: April 2022

AbstractMetal complexes have shown great potential in cancer immunotherapy. This review briefly introduces the basic concepts and strategies of cancer immunotherapy and summarizes the recent discoveries on the immune effects of traditional platinum‐based anticancer compounds. In addition, we also outline the latest research progresses on metal complexes for cancer immunotherapy focusing on platinum, ruthenium, iridium, rhenium and copper complexes. Finally, the research perspectives and unsolved problems on the applications of metallo‐anticancer agents in cancer immunotherapy are purposed.
Advances in Optical Imaging of Nonalcoholic Fatty Liver Disease
Authors: Shen Yang , Zhou Qianhui , Li Wei , Yuan Lin 
Published: April 2022

AbstractNonalcoholic fatty liver disease (NAFLD), emerging as one of the most common chronic liver diseases including simple steatosis and non‐alcoholic steatohepatitis (NASH), is likely to progress to liver fibrosis and hepatic carcinoma if not treated in time. Therefore, early diagnosis and treatment of NAFLD are necessary. Currently, liver biopsy, as the gold standard for clinical diagnosis of NAFLD, is not widely accepted by patients due to its invasiveness. However, other non‐invasive methods that had been reported for NAFLD (such as magnetic resonance imaging, positron emission tomography, and ultrasound) still suffer from low resolution and sensitivity, which are available as a guide for liver biopsy sometimes. As a non‐invasive modality with high spatiotemporal resolution and superior sensitivity, optical imaging methods have been widely favored in recent years, mainly including fluorescence imaging, photoacoustic imaging, and bioluminescence imaging. With these optical imaging approaches, a series of optical probes based on optical and molecular‐specific design have been developed for the biomarker diagnosis and research of diseases. In this review, we summarize the existing non‐invasive optical imaging probes for the detection of biomarkers in NAFLD, including microenvironment (viscosity, polarity), ROS, RSS, ions, proteins, and nucleic acids. Design strategies for optical imaging probes and their applications in NAFLD bioimaging are discussed and focused on. We also highlight the potential challenges and prospects of designing new generations of optical imaging probes in NAFLD studies, which will further enhance the diversity, practicality, and clinical feasibility of NAFLD research.
Optimal burn‐in policy for mixed population of products under two‐dimensional combination warranty
Authors: Wei Yinzhao , Liu Sanyang , Ling Xiaoliang , Shen Lijuan 
Published: April 2022

AbstractIn actual production, the heterogeneity of products is ubiquitous. This article considers a new burn‐in model for repairable products sold with a two‐dimensional combination warranty . It is assumed that the products are heterogeneous and that all failures during the warranty period are repaired by the minimal repair at subpopulations level. Performance‐based and cost‐based models are developed to obtain optimal burn‐in policies under the non‐renewing two‐dimensional combination warranty policy, respectively. First, under mild conditions, we show that the optimal burn‐in time and the optimal usage rate should be as high as possible, provided that the burn‐in time and the burn‐in usage rate have the upper bounds. Second, we analyze the influence of incorrect use of the failure rate function instead of the intensity function on performance‐based and cost‐based models. Finally, we compare the optimal burn‐in policies of the two models. One illustrative example and relevant sensitivity analysis are also presented.
Genetic engineering of the branched‐chain fatty acid biosynthesis pathway to enhance surfactin production from Bacillus subtilis
Authors: Jing Ya‐Fei , Wei Hao‐Xun , Liu Fang‐Fang , Liu Yi‐Fan , Zhou Lei , Liu Jin‐Feng , Yang Shi‐Zhong , Zhang Hui‐Zhan , Mu Bo‐Zhong 
Published: April 2022

AbstractSurfactin, which is composed of a β‐hydroxy fatty acid chain and a peptide ring, has drawn considerable attention due to its potential applications in the biomedicine, bioremediation, and petroleum industries. However, the low yield of surfactin from wild strains still restricts its industrial applications. In this study, eight genes relevant to the fatty acid biosynthesis pathway were targeted to enhance surfactin production, and high surfactin‐yielding strains with potential industrial applications were obtained. When ldeHA and acc were co‐overexpressed, the surfactin yield of recombinant strains TDS8 and TPS8 increased to 1.55‐ and 1.19‐fold of their parental strains, respectively, again proving that the conversion of acetyl‐coenzyme A (CoA) to malonyl‐CoA is the rate‐limiting step in fatty acid biosynthesis. Furthermore, changes in surfactin isoforms of recombinant strain TPS8 suggest that the fatty acid precursor synthesis pathway can be modified to improve the proportion of different isoforms. In addition, the deletion of lpdV, which is responsible for the conversion of α‐ketoacyl‐CoA precursors, resulted in a sharp decrease in surfactin production, further demonstrating the importance of branched‐chain fatty acid biosynthesis in surfactin production. This work will facilitate the design and construction of more efficiently engineered strains for surfactin production and further extend industrial applications.
An overview of synthetic methods and applications of photoluminescence properties of carbon quantum dots
Authors: Rawat Pooja , Nain Parul , Sharma Shaveta , Sharma Parshant Kumar , Malik Vidhu , Majumder Sudip , Verma Ved Prakash , Rawat Varun , Rhyee Jong Soo 
Published: April 2022

AbstractCarbon quantum dots (CQDs) are promising carbonaceous nanomaterials fortuitously discovered in 2004. CQDs are the rising stars in the nanotechnology ensemble because of their unique properties and widespread applications in sensing, imaging, medicine, catalysis, and optoelectronics. CQDs are notable for their excellent solubility and effective luminescence and, as a result, they are also known as carbon nanolights. Many strategies are used for the efficient and economical preparation of CQDs; however, CQDs prepared from waste or green sustainable methods have greater requirements due to their safety and ease of synthesis. Sustainable chemical strategies for CQDs have been developed, emphasizing green synthetic methodologies based on ‘top‐down’ and ‘bottom‐up’ approaches. This review summarizes many such studies relevant to the development of sustainable methods for photoluminescent CQDs. Furthermore, we have emphasized recent advances in CQDs' photoluminescence applications in chemical and biological fields. Finally, a brief overview of synthetic processes using the green source and their associated applications are tabulated, providing a clear understanding of the new optoelectronic materials.
Pareto optimal metabolic engineering for the growth‐coupled overproduction of sustainable chemicals
Authors: Amaradio Matteo N. , Ojha Varun , Jansen Giorgio , Gulisano Massimo , Costanza Jole , Nicosia Giuseppe 
Published: April 2022

AbstractOur research aims to help industrial biotechnology develop a sustainable economy using green technology based on microorganisms and synthetic biology through two case studies that improve metabolic capacity in yeast models Yarrowia lipolytica (Y. lipolytica) and Saccharomyces cerevisiae (S. cerevisiae). We aim to increase the production capacity of beta‐carotene (β‐carotene) and succinic acid, which are among the highest market demands due to their versatile use in numerous consumer products. We performed simulations to identify in silico ranking of strains based on multiple objectives: the growth rate of yeast microorganisms, the number of used chromosomes, and the production capability of β‐carotene (for Y. lipolytica) and succinate (for S. cerevisiae). Our multiobjective optimization methodology identified notable gene deletions by searching a vast solution space to highlight near‐optimal strains on Pareto Fronts, balancing the above‐cited three objectives. Moreover, preserving the metabolic constraints and the essential genes, this study produced robust results: seven significant strains of Y. lipolytica and seven strains of S. cerevisiae. We examined gene knockout to study the function of genes and pathways. In fact, by studying the frequently silenced genes, we found that when the GPH1 gene is knocked out in S. cerevisiae, the isocitrate lyase enzyme is activated, which converts the isocitrate into succinate. Our goals are to simplify and facilitate the in vitro processes. Hence, we present strains with the least possible number of knockout genes and solutions in which the genes are turned off on the same chromosome. Therefore, we present results where the constraints mentioned above are met, like the strains where only two genes are switched off and other strains where half of the knockout genes are on the same chromosome. This study offers solutions for developing an efficient in vitro mutagenesis for microorganisms and demonstrates the efficiency of multiobjective optimization in automatizing metabolic engineering processes.
Spatio‐Temporal Photoactivation of Cytotoxic Proteins
Authors: Cruz‐Samperio Raquel , Mart Robert J. , Luk Louis Y. P. , Tsai Yu‐Hsuan , Jones Arwyn T. , Allemann Rudolf K. 
Published: April 2022

AbstractProtein therapeutics offer exquisite selectivity in targeting cellular processes and behaviors, but are rarely used against non‐cell surface targets due to their poor cellular uptake. While cell‐penetrating peptides can be used to deliver recombinant proteins to the cytosol, it is generally difficult to selectively deliver active proteins to target cells. Here, we report a recombinantly produced, intracellular protein delivery and targeting platform that uses a photocaged intein to regulate the spatio‐temporal activation of protein activity in selected cells upon irradiation with light. The platform was successfully demonstrated for two cytotoxic proteins to selectively kill cancer cells after photoactivation of intein splicing. This platform can generically be applied to any protein whose activity can be disrupted by a fused intein, allowing it to underpin a wide variety of future protein therapeutics.