bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid detection of Salmonella by low‐field NMR biosensor using long‐arm functional magnetic nanoprobes
Authors: Dong Qiuling , Liang Xuehua , Xiong Chunhong , Huang Ganhui , Zhang Jinsheng 
Published: October 2022

SummaryDeveloping rapid detection tools and methods for foodborne pathogens has always been a research hotspot, which is undoubtedly very important for food safety. In this study, the preparation of functional magnetic nanoprobes and their application to detect target pathogens in a complex matrix by low‐field nuclear magnetic resonance (LF‐NMR) is demonstrated. The long‐arm functional magnetic nanoprobes were constructed by modification with polyethylene glycol (PEG) onto carboxyl‐modified magnetic nanoparticles (MNPs) and then reaction with biotin (Bio). Under the optimal conditions, the lower limit that can be detected in the PBS buffer system is 100 CFU/mL, and the detection of the actual sample matrix can be achieved 102 CFU/g. The practical application of LF‐NMR sensors to detect harmful pathogenic bacteria in food provides an efficient way of monitoring dangerous pathogenic diseases.
Functioning of structurally diverse families living with adolescents and children with chronic disease: A metasynthesis
Authors: Neris Rhyquelle Rhibna , Bolis Letícia Onelli , Leite Ana Carolina Andrade Biaggi , Alvarenga Willyane de Andrade , Garcia‐Vivar Cristina , Nascimento Lucila Castanheira 
Published: October 2022

AbstractPurposeThe diagnosis of children and adolescents with a chronic disease may affect the entire family system. When families have diverse structures, additional tensions can be present and affect the balance of family functioning. This metasynthesis aims to analyze and synthesize qualitative evidence on the functioning of structurally diverse families who live with adolescents and children with chronic disease.DesignQualitative metasynthesis.MethodsSystematic searches up to 2021 were performed in PubMed, CINAHL, PsycINFO, SCOPUS, LILACS, and Web of Science and supplemented by manual search strategies. It followed guidelines from the statement in the Enhancing Transparency in Reporting the Synthesis of Qualitative Research (ENTREQ). A quality appraisal of each study was undertaken using the Critical Appraisal Skills Programme. Data synthesis was conducted according to the thematic synthesis approach.FindingsOf a total of 6538 references identified, 9 studies were included in the metasynthesis. The thematic synthesis enabled the construction of three analytical themes: “Family structural changes and weakened co‐parenting”; “Family rearrangements and the challenges faced by families”; and “Committed to healthy family functioning for the child's well‐being: Searching for family homeostasis”.ConclusionsThe themes showed that the causes of the rupture in the family unit interfere in family functioning, making it ineffective. In most families, family functioning is centered on the mothers. Faced with the need to care for children and adolescents and to control chronic disease, structurally diverse families need to adjust their family functioning and search for family homeostasis.Clinical RelevanceThe results of this review can support nurses to target their care toward these families and formulate effective interventions that promote, strengthen, or maintain the healthy functioning of these families.
Putting life on hold: A longitudinal phenomenological‐hermeneutic study of living with [or close to someone with] an enterocutaneous fistula before and after reconstructive surgery
Authors: Härle Karolina , Börjeson Sussanne , Hallböök Olof , Myrelid Pär , Thylèn Ingela 
Published: October 2022

AbstractAims and ObjectivesIlluminate meanings of living with [or close to someone with] an enterocutaneous fistula before and after reconstructive surgery.BackgroundEnterocutaneous fistula is a serious condition affecting the well‐being and social life of both patients and families, sometimes for several years.DesignA longitudinal qualitative design.MethodsPatients and families (n = 14) were followed with dyadic interviews at three occasions, conducted 2017–2020. The interviews were analysed with a phenomenological‐hermeneutic approach. COREQ guidelines were followed.ResultLiving with an enterocutaneous fistula was explained as life being put on hold, while living in a bubble for an uncertain time. This bubble meant facing an unpredictable and restricted life where the dyads were forced to take control over the situation despite being vulnerable inside, striving to resume normality. The patients dealt with never‐ending symptoms such as leakage from the fistula, pain and fatigue, while the family supported with practical matters and just being close. Dependency on intravenous fluids resulted in social isolation, which caused mode swings and depressiveness. In this situation, healthcare professionals often became a substitute for other interactions, but the lack of understanding about the dyads' situation, affected their trust in the healthcare. Despite all, they still had belief in the future, the patients having higher expectations than the family.ConclusionsLiving with an enterocutaneous fistula meant a daily life struggling with many limitations. This implies that the transition was associated with difficulties and the dyads strived to accept their situation.Relevance to Clinical PracticeThe findings indicate that these patients must be cared for with a multidisciplinary approach. A person‐centred health plan could impact on the dyads' feeling of control and thereby making them less dependent on healthcare. Every patient should have their own contact nurse and be offered psychological support.No Patient or Public ContributionNot applicable due to the current method.
The characteristics of pre‐excitation syndrome concomitant with atrial tachyarrhythmia and the effect of radiofrequency ablation
Authors: Cui Chang , Li Zhaomin , Ju Weizhu , Yang Gang , Gu Kai , Liu Hailei , Li Mingfang , Chen Hongwu , Wang Jing , Chen Minglong 
Published: October 2022

AbstractBackgroundWolff‐Parkinson‐White (WPW) concomitant with atrial tachyarrhythmia (ATA) has not been systemically characterized.MethodsDetailed electroanatomical mapping of the right atrium (RA) and/or left atrium (LA) was performed using three‐dimensional mapping and the accessory pathway (AP) was mapped.ResultsWPW syndrome with ATA was diagnosed in 11 patients (median age 60 years). The characteristic of unidirectional anterograde conduction over the AP was displayed in nine patients, six of whom were intermittent. Sustained atrial tachycardia, that is, counterclockwise atrial flutter (AFL) with a median tachycardia cycle length (TCL) of 225 (220–275) ms, was mapped in eight patients; furthermore, “figure 8” right atrial reentry was mapped with TCL 250 ms in one patient with a surgical history of ventricular septal defect repair. The remaining two patients underwent mitral annulus‐dependent AT after paroxysmal atrial fibrillation (PAF) ablation and LA micro‐reentry AT, respectively. In four patients, the location of the APs was left posterior. Left‐lateral APs were identified in four patients. The locations of the APs in the remaining three patients were the right posterior and middle septum. All ATAs and APs were successfully ablated. After a median follow‐up of 37 (15–72) months, no anterograde conduction over the AP was recorded, new onset of PAF was recorded in three patients, and all of them underwent circumferential pulmonary vein isolation.ConclusionsWPW with concomitant ATA frequently had continuous anterograde conduction over the AP with a rapid ventricular rate. Most WPWs displayed the characteristic of unidirectional anterograde conduction.
Engineering triacylglycerol accumulation in duckweed (Lemna japonica)
Authors: Liang Yuanxue , Yu Xiao‐Hong , Anaokar Sanket , Shi Hai , Dahl William B. , Cai Yingqi , Luo Guangbin , Chai Jin , Cai Yuanheng , Mollá‐Morales Almudena , Altpeter Fredy , Ernst Evan , Schwender Jorg , Martienssen Robert A. , Shanklin John 
Published: October 2022

SummaryDuckweeds are amongst the fastest growing of higher plants, making them attractive high‐biomass targets for biofuel feedstock production. Their fronds have high rates of fatty acid synthesis to meet the demand for new membranes, but triacylglycerols (TAG) only accumulate to very low levels. Here we report on the engineering of Lemna japonica for the synthesis and accumulation of TAG in its fronds. This was achieved by expression of an estradiol‐inducible cyan fluorescent protein‐Arabidopsis WRINKLED1 fusion protein (CFP‐AtWRI1), strong constitutive expression of a mouse diacylglycerol:acyl‐CoA acyltransferase2 (MmDGAT), and a sesame oleosin variant (SiOLE(*)). Individual expression of each gene increased TAG accumulation by 1‐ to 7‐fold relative to controls, while expression of pairs of these genes increased TAG by 7‐ to 45‐fold. In uninduced transgenics containing all three genes, TAG accumulation increased by 45‐fold to 3.6% of dry weight (DW) without severely impacting growth, and by 108‐fold to 8.7% of DW after incubation on medium containing 100 μm estradiol for 4 days. TAG accumulation was accompanied by an increase in total fatty acids of up to three‐fold to approximately 15% of DW. Lipid droplets from fronds of all transgenic lines were visible by confocal microscopy of BODIPY‐stained fronds. At a conservative 12 tonnes (dry matter) per acre and 10% (DW) TAG, duckweed could produce 350 gallons of oil/acre/year, approximately seven‐fold the yield of soybean, and similar to that of oil palm. These findings provide the foundation for optimizing TAG accumulation in duckweed and present a new opportunity for producing biofuels and lipidic bioproducts.
Response of sugar metabolism in the cotyledons and roots of Ricinus communis subjected to salt stress
Authors: Li Y. , Chu Y. , Yao K. , Shi C. , Deng X. , Lin J. 
Published: October 2022

Abstract

Ricinus communis is an important oilseed crop worldwide and is also considered one of the best potential plants for salt‐affected soil improvement in northeast China. However, little is known about photosynthesis and carbohydrate metabolism in this plant, nor the distribution of carbohydrates in cotyledons and roots under salinity stress.

In the present study, seedling growth, gas exchange parameters (PN, E, gs and Ci), carbohydrate (fructose, sucrose, glucose, soluble sugar and starch) metabolism and related enzymes and genes were measured in Ricinus plants.

Under salt stress, PN of cotyledons decreased significantly (P < 0.05), resulting in weak photosynthetic capacity. Furthermore, salt stress increased sucrose and glucose content in cotyledons, but decreased soluble sugar and starch content. However, sucrose increased and starch decreased in roots. This may be correlated with the increasing sugar metabolism under salinity, including notable changes in sugar‐related enzyme activities (SPS, SuSy, α‐amylase and β‐amylase) and gene expression of RcINV, RcSUS, RcAmY, RcBAM and RcGBE1.

The results suggest that salinity reduces photosynthesis of cotyledons, alters carbohydrate allocation between cotyledons and roots and also promotes starch utilization in cotyledons and starch biosynthesis in roots, leading to a functional imbalance between cotyledons and roots. Together, these findings provide insights into the crucial role of sugar metabolism in improving salt‐tolerance of Ricinus during the early seedling growth stage.
Trajectories of low‐income mothers’ and fathers’ engagement in learning activities and child socioemotional skills in middle childhood
Authors: Fagan Jay , Cabrera Natasha 
Published: October 2022

AbstractUsing Fragile Families and Child Wellbeing data (N = 3259), the current study examined Black and Latinx mothers’ and fathers’ trajectories of engagement in learning activities (e.g., storytelling) from infancy to age 5, and whether those trajectories predicted socioemotional skills at age 9, predictors of the trajectories (poverty, mother‐father nonresidence, temperament, race/ethnicity), and moderators of the trajectories. Mothers’ and fathers’ learning activities decreased significantly over time as children got older. Higher rates of decline in fathers’ engagement in learning activities over time significantly predicted lower socioemotional skills. Mother‐father nonresidence during infancy was associated significantly with higher rates of decline in mothers’ and fathers’ learning activities. Difficult temperament moderated the association between fathers’ trajectories and child outcomes.
Class A lysophosphatidic acid acyltransferase 2 from Camelina sativa promotes very long‐chain fatty acids accumulation in phospholipid and triacylglycerol
Authors: Yin Yongtai , Raboanatahiry Nadia , Chen Kang , Chen Xinfeng , Tian Tian , Jia Jia , He Hongsheng , He Jianjie , Guo Zhenyi , Yu Longjiang , Li Maoteng 
Published: October 2022

SUMMARYVery long‐chain fatty acids (VLCFAs) are important industrial raw materials and can be produced by genetically modified oil plants. For a long time, class A lysophosphatidic acid acyltransferase (LPAT) was considered unable to promote the accumulation of VLCFA in oil crops. The bottlenecks that the transgenic high VLCFA lines have an oil content penalty and the low amount of VLCFA in phosphatidylcholine remains intractable. In the present study, a class A LPAT2 from Camelina sativa (CsaLPAT2) promoting VLCFAs accumulation in phospholipid was found. Overexpression of CsaLPAT2 alone in Arabidopsis seeds significantly increased the VLCFA content in triacylglycerol, including C20:0, C20:2, C20:3, C22:0, and C22:1. The proportion of phosphatidic acid molecules containing VLCFAs in transgenic seeds reached up to 45%, which was 2.8‐fold greater than that in wild type. The proportion of phosphatidylcholine and diacylglycerol molecules containing VLCFAs also increased significantly. Seed size in CsaLPAT2 transgenic lines showed a slight increase without an oil content penalty. The total phospholipid content in the seed of the CsaLPAT2 transgenic line was significantly increased. Furthermore, the function of class A LPAT in promoting the accumulation of VLCFAs is conserved in the representative oil crops of Brassicaceae, such as C. sativa, Arabidopsis thaliana, Brassica napus, Brassica rapa, and Brassica oleracea. The findings of this study provide a promising gene resource for the production of VLCFAs.
Photosynthetic recovery in drought‐rehydrated grapevines is associated with high demand from the sinks, maximizing the fruit‐oriented performance
Authors: Patono Davide L. , Said‐Pullicino Daniel , Eloi Alcatrāo Leandro , Firbus Andrea , Ivaldi Giorgio , Chitarra Walter , Ferrandino Alessandra , Ricauda Aimonino Davide , Celi Luisella , Gambino Giorgio , Perrone Irene , Lovisolo Claudio 
Published: October 2022

SUMMARYTo understand how grapevine sinks compete with each other during water stress and subsequent rehydration, carbon (C) allocation patterns in drought‐rehydrated vines (REC) at the beginning of fruit ripening were compared with control vines maintained under drought (WS) or fully irrigated (WW). In the 30 days following rehydration, the quantity and distribution of newly fixed C between leaves, roots and fruits was evaluated through 13CO2 pulse‐labeling and stable isotope ratio mass spectrometry. REC plants diverted the same percentage of fixed C towards the berries as the WS plants, although the percentage was higher than that of WW plants. Net photosynthesis (measured simultaneously with root respiration in a multichamber system for analysis of gas exchange above‐ and below‐ground) was approximately two‐fold greater in REC compared to WS treatment, and comparable or even higher than in WW plants. Maximizing C assimilation and delivery in REC plants led to a significantly higher amount of newly fixed C compared to both control treatments, already 2 days after rehydration in root, and 2 days later in the berries, in line with the expression of genes responsible for sugar metabolism. In REC plants, the increase in C assimilation was able to support the requests of the sinks during fruit ripening, without affecting the reserves, as was the case in WS. These mechanisms clarify what is experienced in fruit crops, when occasional rain or irrigation events are more effective in determining sugar delivery towards fruits, rather than constant and satisfactory water availabilities.
Explaining anomalies of B-physics, muon $$g-2$$ and W mass in R-parity violating MSSM with seesaw mechanism
Authors: Zheng Min-Di , Chen Feng-Zhi , Zhang Hong-Hao 
Published: October 2022

AbstractThe recent experimental results including $$R_{K^{(*)}}$$

R

K

(

∗
)

, $$R_{D^{(*)}}$$

R

D

(

∗
)

, $$(g-2)_\mu $$

(
g
-
2
)

μ

and W mass show deviations from the standard model (SM) predictions, implying the clues of new physics (NP). In this work, we investigate the explanations of these anomalies in the R-parity violating minimal supersymmetric standard model (RPV-MSSM) extended with the inverse seesaw mechanism. The non-unitarity extent $$\eta _{ee}$$

η

ee

and the loop corrections from the interaction $$\lambda '\hat{L} \hat{Q} \hat{D}$$

λ
′

L
^

Q
^

D
^

are utilized to raise the prediction of W mass through muon decays. We also find that the interaction $$\lambda '\hat{L} \hat{Q} \hat{D}$$

λ
′

L
^

Q
^

D
^

involved with right-handed (RH)/singlet (s)neutrinos can explain the $$R_{K^{(*)}}$$

R

K

(

∗
)

and $$R_{D^{(*)}}$$

R

D

(

∗
)

anomalies simultaneously when considering nonzero $$\lambda '_{1jk}$$

λ

1
j
k

′

. For nonzero $$\lambda '_{2jk}$$

λ

2
j
k

′

, this model fulfills the whole $$b\rightarrow s\ell ^+\ell ^-$$

b
→
s

ℓ
+

ℓ
-

fit but cannot be accordant with $$R_{D^{(*)}}$$

R

D

(

∗
)

measurements. The explanations in both cases are also favored by $$(g-2)_\mu $$

(
g
-
2
)

μ

data, neutrino oscillation data and the relevant constraints we scrutinized. Furthermore, this model framework can be tested in future experiments covering, e.g., the predicted lepton flavor violations (LFV) at Belle II and the Future Circular Collider with $$e^+e^-$$

e
+

e
-

beams (FCC-ee), as well as the heavy neutrinos at future colliders.
Gravitational wave of the Bianchi VII universe: particle trajectories, geodesic deviation and tidal accelerations
Authors: Osetrin Konstantin , Osetrin Evgeny , Osetrina Elena 
Published: October 2022

AbstractFor the gravitational wave model based on the type III Shapovalov wave space-time, test particle trajectories and the exact solution of geodesic deviation equations for the Bianchi type VII universe are obtained. Based on the found 4-vector of deviation, tidal accelerations in a gravitational wave are calculated. For the obtained solution in a privileged coordinate system, an explicit form of transformations into a synchronous reference system is found, which allows time synchronization at any points of space-time with separation of time and spatial coordinates. The synchronous reference system used is associated with a freely falling observer on the base geodesic. In a synchronous coordinate system, an explicit form of the gravitational wave metric, a 4-vector of geodesic deviation, and a 4-vector of tidal accelerations in a gravitational wave are obtained. The exact solution describes a variant of the primordial gravitational wave. The results of the work can be used to study the plasma radiation generated by tidal accelerations of a gravitational wave.
Variation in Patient-Reported Decision-Making Roles in the Last Year of Life among Patients with Metastatic Cancer: A Longitudinal Study
Authors: Ozdemir Semra , Chaudhry Isha , Tan Si Ning Germaine , Teo Irene , Malhotra Chetna , Malhotra Rahul , Finkelstein Eric Andrew 
Published: October 2022

Objective We investigated the variation in patient-reported decision-making roles in the past year of life among patients with metastatic solid cancer and the associations of these roles with patient quality of life and perceived quality of care. Methods We used the last year of life data of 393 deceased patients from a prospective cohort study. Patients reported their decision-making roles, quality of life (emotional well-being, spiritual well-being, and psychological distress) and perceived quality of care (care coordination and physician communication) every 3 months until death. We used mixed effects linear regressions to investigate the associations of decision-making roles with patients’ quality of life and perceived quality of care. Results The most reported roles, on average, were patient-led (37.9%) and joint (23.4%; with physicians and/or family caregivers) decision making, followed by no patient involvement (14.8%), physician/family-led (12.9%), and patient alone (11.0%) decision making. Patient level of involvement in decision making decreased slightly as death approached ( P < 0.05). Compared with no patient involvement, joint decision making was associated with better emotional well-being (β [95% confidence interval] = 1.02 [0.24, 1.81]), better spiritual well-being (1.48 [0.01, 2.95]), lower psychological distress (−1.99 (−3.21, −0.77]), higher perceived quality of care coordination (5.04 [1.24, 8.85]), and physician communication (5.43 [1.27, 9.59]). Patient-led decision making was associated with better spiritual well-being (1.69 [0.24, 3.14]) and higher perceived quality of care coordination (6.87 [3.17, 10.58]) and physician communication (6.21 [2.15, 10.27]). Conclusion Joint and patient-led decision-making styles were reported by 61% of the patients and were associated with better quality of life and quality of care. A decrease in the level of patient involvement over time indicates reliance on family and physicians as death approached. Highlights Among patients with metastatic cancer, the level of patient involvement in decision making decreased slightly as death approached. Joint decision making of patients with their physicians and/or family caregivers and patient-led decision making were associated with better quality of life and perceived quality of care. Patients with metastatic cancer should be encouraged to be involved in decision making together with their physicians and/or family caregivers to potentially improve their end-of-life experience.
Direct Oral Anticoagulants Versus Warfarin for Venous Thromboembolism Prophylaxis in Patients With Nephrotic Syndrome: A Retrospective Cohort Study
Authors: Tijani Aminat , Coons Eric M. , Mizuki Britta , Dermady Miranda , Stanilova Katerina , Casey Ashley L. , Alqudsi Muhannad , Gastanaduy Mariella , Elmayan Ardem , Bamnolker Adi , Velez Juan Carlos Q. 
Published: October 2022

Background: Evidence supporting venous thromboembolism (VTE) prophylaxis with direct oral anticoagulants (DOACs) in patients with nephrotic syndrome (NS) is limited to case reports. Objective: The purpose of this study was to compare bleeding and thromboembolic events in this population. Methods: A retrospective cohort study was conducted in adults with NS initiated on a DOAC or warfarin for VTE prophylaxis between January 2013 and July 2021 within the Ochsner Health System. Patients with study drug exposure within the preceding 7 days, acute VTE within the preceding 6 months, or ≤7 days of study drug exposure were excluded. The primary outcome was the composite rate of major bleeding and clinically relevant nonmajor bleeding. Secondary outcomes included time to major bleeding and rate of new thromboembolic events. This study was approved by the Ochsner Health System Institutional Review Board. Results: Twenty-five DOAC and 19 warfarin patients were included. The primary outcome occurred in 8% vs 26.3% ( P = 0.21) of patients treated with a DOAC or warfarin, respectively, and was driven by major bleeding (4% vs 21%, P = 0.25). Other secondary outcomes were similar between cohorts. The study was limited by a small sample size. Conclusion and Relevance: Use of DOACs for VTE prophylaxis resulted in a nonstatistically significant, but clinically relevant lower rate of major bleeding compared to warfarin. This study provides comparative data showing safe and effective use of DOACs in patients with NS. Prospective, randomized studies are needed to confirm results.