bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elucidating the patterns of pleiotropy and its biological relevance in maize
Authors: Khaipho-Burch Merritt , Ferebee Taylor , Giri Anju , Ramstein Guillaume , Monier Brandon , Yi Emily , Romay M. Cinta , Buckler Edward S. 
Published: July 2022

AbstractPleiotropy - when a single gene controls two or more seemingly unrelated traits - has been shown to impact genes with effects on flowering time, leaf architecture, and inflorescence morphology in maize. However, the genome-wide impact of true biological pleiotropy across all maize phenotypes is largely unknown. Here we investigate the extent to which biological pleiotropy impacts phenotypes within maize through GWAS summary statistics reanalyzed from previously published metabolite, field, and expression phenotypes across the Nested Association Mapping population and Goodman Association Panel. Through phenotypic saturation of 120,597 traits, we obtain over 480 million significant quantitative trait nucleotides. We estimate that only 1.56-32.3% of intervals show some degree of pleiotropy. We then assessed the relationship between pleiotropy and various biological features such as gene expression, chromatin accessibility, sequence conservation, and enrichment for gene ontology terms. We find very little relationship between pleiotropy and these variables when compared to permuted pleiotropy. We hypothesize that biological pleiotropy of common alleles is not widespread in maize and is highly impacted by nuisance terms such as population structure and linkage disequilibrium. Natural selection on large standing natural variation in maize populations may target wide- and large-effect variants, leaving the prevalence of detectable pleiotropy relatively low.Author SummaryThe genetic basis of complex traits has been thought to exhibit pleiotropy, which is the notion that a single locus can control two or more unrelated traits. Widespread reports in the human disease literature show genomic signatures of pleiotropic loci across many traits. However, little is known about the prevalence and behavior of pleiotropy in maize across a large number of phenotypes. Using association mapping of common alleles in over one hundred thousand traits, we determine how pleiotropic each region was and use these quantitative scores to functionally characterize each region of the genome. Our results show little evidence that pleiotropy is a common phenomenon in maize. We observed that maize does not exhibit the same pleiotropic characteristics as human diseases in terms of prevalence, gene expression, chromatin accessibility, or sequence conservation. Rather than pervasive pleiotropy, we hypothesize that strong selection on large and wide effect loci and the need for trait independence at the gene level keep the prevalence of pleiotropy low, thus, allowing for the adaptation of maize varieties to novel environments and conditions.
Male Guinea baboons are oblivious to their females’ whereabouts
Authors: Treschnak Dominique , Zinner Dietmar , Fischer Julia 
Published: July 2022

AbstractIn group-living species, evolution puts a premium on the ability of individuals to track the state, whereabouts, and interactions of others. The value of social information might vary with the degree of competition within and between groups, however. We investigated male monitoring of female location in wild Guinea baboons (Papio papio). Guinea baboons live in socially tolerant multi-level societies with one-male-units comprising 1-6 females and young at the core. Using field playback experiments, we first tested whether male Guinea baboons (N=14) responded more strongly to playbacks of associated vs. non-associated females, which was the case. In the second and core experiment, we tested whether males (N=22 males, N=62 trials) keep track of the whereabouts of associated females by playing back unit females’ calls from locations that were either consistent or inconsistent with the actual position of the female. Contrary to predictions, males responded equally strongly in both conditions. While males seem to recognize their females by voice, they might lack the attention or motivation to track their females’ movement patterns. These results reinforce the view that the value of social information may vary substantially with the distribution of power in a society. While highly competitive regimes necessitate high attention to deviations from expected patterns, egalitarian societies allow for a certain degree of obliviousness.
Isoform- and ligand-specific modulation of the adhesion GPCR ADGRL3/Latrophilin3 by a synthetic binder
Authors: Kordon Szymon P. , Dutka Przemysław , Adamska Justyna M. , Bandekar Sumit J. , Leon Katherine , Adams Brock , Li Jingxian , Kossiakoff Anthony A. , Araç Demet 
Published: July 2022

ABSTRACTAdhesion G protein-coupled receptors (aGPCRs) are cell-surface proteins with large extracellular regions that bind to multiple ligands to regulate key biological functions including neurodevelopment and organogenesis. Modulating a single function of a specific aGPCR isoform while affecting no other function and no other receptor is not trivial. Here, we engineered an antibody, termed LK30, that binds to the extracellular region of the aGPCR ADGRL3, and specifically acts as an agonist for ADGRL3 but not for its isoform, ADGRL1. The LK30/ADGRL3 complex structure revealed that the LK30 binding site on ADGRL3 overlaps with the binding site for an ADGRL3 ligand – teneurin. In cellular-adhesion assays, LK30 specifically broke the trans-cellular interaction of ADGRL3 with teneurin, but not with another ADGRL3 ligand – FLRT3. Our work provides proof of concept for the modulation of isoform- and ligand-specific aGPCR functions using unique tools, and thus establishes a foundation for the development of fine-tuned aGPCR-targeted therapeutics.
Molecular characterization of extended-spectrum beta-lactamase-producing extra-intestinal pathogenic Escherichia coli isolated in a university teaching hospital Dakar-Senegal
Authors: Dossouvi Komla Mawunyo , Ba Bissoume Sambe , Lo Gora , Cissé Abdoulaye , Ba-Diallo Awa , Ndiaye Issa , Dieng Assane , Ndiaye Serigne Mbaye Lo , Fall Cheikh , Tine Alioune , Karam Farba , Diagne-Samb Habsa , Ngom-Cisse Safietou , Diop-Ndiaye Halimatou , Toure-Kane Coumba , Gaye-Diallo Aïssatou , Mboup Souleymane , Boye Cheikh Saad Bouh , Dièye Yakhya , Seck Abdoulaye , Camara Makhtar 
Published: July 2022

AbstractExtra-intestinal pathogenic Escherichia coli (ExPEC), a predominant Gram-negative bacterial pathogen, express a wide range of virulence factors and is responsible of several diseases including urinary tract infections (UTI), nosocomial pneumonia, bacteremia, and neonatal meningitis. ExPEC isolates are often multidrug resistant (MDR) and clones producing extended-spectrum beta-lactamases (ESBL) are increasingly reported all over the world.Seventy-eight clinical ExPEC strains were selected for this study. The majority was from UTIs (n=51), while the rest (n=27) was from pus, sputum, bronchial fluid and vaginal samples (non-uropathogenic ExPEC). Interestingly, 49 out of the 78 ExPEC isolates where considered as community-acquired (CA) and 29 hospital-acquired (HA) bacteria. Antibiotic susceptibility testing was performed using the Kirby-Bauer disc diffusion method. Standard polymerase chain reaction (PCR) was used to screen major ESBL genes (blaCTX-M, blaOXA-1, blaTEM, blaSHV) and blaCTX-M variants (blaCTX-M-1, blaCTX-M-9, blaCTX-M-15, blaCTX-M-25).All the ExPEC strains were resistant to ampicillin, ticarcillin, amoxicillin/clavulanic acid combination, cefalotin, cefotaxime, ceftazidime, cefepime and aztreonam, but showed a high susceptibity to fosfomycin (98.7%, n = 77), ertapenem (96.2%, n = 75), and imipenem (100%). Moreover, isolates harbored at least one ESBL gene, including blaCTX-M (98.7%), blaOXA-1 (78.2%), blaTEM (44.9%) and blaSHV (3.8%). The CTX-M variants were also found with the predominance of blaCTX-M-1 (90.9%) and blaCTX-M-15 (90.9%) followed by blaCTX-M-9 (11.7%), while blaCTX-M-25 was not detected.Despite the resistance to most of the tested antibiotics, ExPEC isolates showed fortunately a good susceptibility to fosfomycin and carbapenems. blaCTX-M (blaCTX-M1, blaCTX-M15) and blaOXA-1 seem to be E. coli major ESBL genes circulating in Senegal. No significant difference was noted when comparing prevalence of ESBL genes detected from CA and HA strains, and from UPEC and non-uropathogenic ExPEC. The high level of resistance to antimicrobials observed stresses the need of establishing an epidemiological surveillance of antimicrobial resistance in both community and hospital settings.
Circulating androgen regulation by androgen-catabolizing gut bacteria in male mouse gut
Authors: Hsiao Tsun-Hsien , Chou Chia-Hong , Chen Yi-Lung , Wang Po-Hsiang , Brandon-Mong Guo-Jie , Lee Tzong-Huei , Wu Tien-Yu , Li Po-Ting , Li Chen-Wei , Lai Yi-Li , Tseng Yu-Lin , Shih Chao-Jen , Chen Mei-Jou , Chiang Yin-Ru 
Published: July 2022

SummaryAbnormally high circulating androgen levels have been considered a causative factor for benign prostatic hypertrophy and prostate cancer. Recent studies suggested that gut bacteria can alter sex steroid profile of host; however, the underlying mechanisms and bacterial taxa remain elusive.Thauerasp. strain GDN1 is an unusual betaproteobacterium capable of aerobic and anaerobic androgen catabolism in environmental conditions (37°C) resembling the mammalian gut. The strain GDN1 administration to C57BL/6J mice through oral gavage profoundly affected gut bacterial community, along with an approximately 50% reduction in serum androgen level in male mice. Our RT–qPCR results revealed the differential expression of aerobic and anaerobic androgen catabolic genes in the mouse ileum (microaerobic) and caecum (anaerobic), respectively. Furthermore, androgenic ring-cleaved metabolites were detected in the mouse fecal extract. This study discovered that androgen serves as a carbon source of gut microbes and that androgen-catabolizing gut bacteria can modulate host circulating androgen levels.HighlightsThauerasp. strain GDN1 administration through oral gavage regulated mouse serum androgen levels.The biochemical, genetic, and metabolite profile analyses revealed the occurrence of bacterial androgen catabolism in the mouse gut.Androgen catabolism proceeds through the O2-dependent and O2-independent catabolic pathways in mouse ileum and caecum, respectively.A possibility to harnessThauerasp. strain GDN1 as a functional probiotic to treat hyperandrogenism.Graphical AbstractIn briefHsiao et al. found that oral administration of androgen-catabolizingThaueraspecies regulated mouse serum androgen level. They characterized the gut microbe–mediated androgen catabolism through genetic and biochemical analyses. Their discovery portends a possibility of harnessing androgen-catabolic gut bacteria as functional probiotics to treat hyperandrogenism.
Study on the symbiotic development path of traditional villages in the county based on the grey relation analysis: the case of Xunxian County in Henan, China
Authors: Gao Changzheng , Hou Juepin , Gong Jian 
Published: July 2022

PurposeAbundance of cultural resources is a typical feature of traditional villages. It is particularly important to explore the development path of traditional villages from the perspective of cultural resources.Design/methodology/approachBased on symbiosis theory, establish a symbiotic development mechanism of traditional village characteristic cultural elements and use grey relation analysis (GRA) to evaluate the resource advantages of different cultural attributes. This paper proposes the traditional village development model of different symbiotic units in the county. An empirical study is conducted in conjunction with the national historical and cultural city of Xunxian County, Henan, China.FindingsThe results show that (1) according to the different attributes of humanistic cultural resources, artificial cultural resources and natural cultural resources in traditional villages, different symbiotic units can be divided, and advantageous resources can be shared through the symbiotic interface; (2) using GRA to calculate the grey relational degree between cultural resources sequence of different attributes and the sum of cultural resources sequence in the county and define as the active degree of humanistic culture, the inheritance degree of artificial culture and the integrity of natural cultural resources are shown to reflect the status of the inheritance of humanistic cultural resources, the existence of artificial cultural resources, and the protection of natural cultural resources; and (3) a comparative analysis of the active degree, inheritance, and integrity of each symbiotic unit leads to the proposal of three symbiotic models of folklore vitality, characteristic space, and natural ecology, all of which are beneficial to promote the formation of a symbiotic environment.Originality/valueThe main innovation of this paper is to put forward the research scale of symbiosis theory in the field of urban and rural planning at the county level, delimit the symbiosis units of traditional villages at the county level, complete the quantitatively evaluate cultural resources in different symbiotic units with GRA, revealing the symbiotic development mechanism of cultural resources characteristic elements in traditional villages.
Influencing supply chain practice: the action principles approach applied to pandemic risk management
Authors: van Hoek Remko , Lacity Mary , Willcocks Leslie 
Published: July 2022

PurposeThis paper offers a novel approach for conducting impactful research on emerging topics or practices. This method is particularly relevant in the face of emerging phenomena and new dynamics, such as the impact of the COVID-19 pandemic on supply chain risks. Because these new phenomena and dynamics are relatively unexplored, little prior knowledge exists in literature and industry, and they represent a large opportunity and/or challenge to practitioners.Design/methodology/approachThe action principles research (APR) approach, as a newer version of critically engaged research (CER), offers comparison against more traditional empirical or intervention-based research. The authors illustrate the approach with a pandemic risk-management study.FindingsThe APR approach originated in the information technology field. It is highly applicable for researchers who are seeking to more expeditiously support decision making and actioning on new dynamics and emerging topics and practice in supply chain management than is allowed by traditional methods and longitudinal CER.Originality/valueIn the context of ongoing calls for relevance, impact and actionable findings on pandemic risk management, this paper describes an approach to developing timely findings that are actionable for practitioners and that advance science around dynamic and emerging topics or practices. We hope this will grow societal value of research, particularly in the face of the COVID-19 pandemic and the new dynamics and uncertainties that managers face in modern supply chains.
Exploring job resources as predictors of employees' effective coping with job stress
Authors: Zampetakis Leonidas A. , Gkorezis Panagiotis 
Published: July 2022

PurposeThe purpose of this paper is to shed more light on the relative impact of the various workplace resources on employees' effective coping with job stress symptoms, taking into account synergistic and antagonistic effects. The authors used job demands-resources (JD-R) theory as an overarching theoretical framework to test the hypotheses.Design/methodology/approachThe authors used a factorial survey experiment and a within-person design. Data were obtained from a random sample of 97 Greek employees working in public and private organizations. Multilevel modeling regression techniques were used for data analyses. The authors examined the relative effect of various job resources at different levels on employees' ratings of effective coping with job stress symptoms. In parallel, the authors investigated the possible synergistic and antagonistic interactions between the specific job resources.FindingsThe authors found that managers' leadership and humor style, their relationship with subordinates and coworkers’ support had positive effects on employees' coping with job stress. In addition, the authors found that the manager's leadership style interacts with manager–subordinate relationship quality and coworkers’ support as well as the latter interacts with the manager's humor style and manager–subordinate relationship quality. The study model explained 50% of the variance in effective coping with stress ratings.Originality/valueThe study highlights the importance of workplace resources as contextual variables, for the effective coping with stress symptoms at work. It highlights that a combination of workplace resources produces a net effect that was better than would have been expected based solely on the individual performance of these job resources. As such, the research answer calls to attend to the effects of synergistic effects of workplace resources on effective coping with stress symptoms at work.
Organizational ambidexterity and the learning organization: the strategic role of a corporate university
Authors: Lissillour Raphael , Rodriguez-Escobar Javier Alfonso 
Published: July 2022

Purpose
Balancing exploration and exploitation is a strategic challenge for technology-based companies striving to successfully implement ambidexterity in rapidly changing markets. This study aims to look at the extent in which corporate universities can be instrumental in the cross-functional deployment of the resources, capabilities and experience needed to achieve organizational ambidexterity.

Design/methodology/approach
This study is the result of a single case study of ZTE University in China. Data from archives, direct observations, and semi-open interviews have been triangulated and analyzed with pattern matching technique.

Findings
This study analyzed the development of capabilities allowing the strategic combinations of exploration and exploitation, and to clearly witness how the corporate university was dynamically linked with those development.

Originality/value
The empirical results offer new insights on the most relevant capabilities for technology-based companies and notably those that are more likely to be exploited through a corporate university.
Willingness to purchase refurbished products sold online: a qualitative inquiry of young consumers from an emerging market
Authors: Gaur Jighyasu , Srivastava Ankur , Gupta Ritu 
Published: July 2022

Purpose
The literature provides evidence of consumers’ willingness to purchase (WTP) refurbished products. However, the studies focusing on young consumers’ (18–24 years) purchasing behaviour of refurbished products from online platforms/stores are limited. Therefore, the purpose of this study is to conduct a qualitative study to identify the key attributes/factors that influence young consumers’ WTP refurbished products.

Design/methodology/approach
Two qualitative techniques, focus group discussion (FGD) and depth interviews (DI), are used for data collection. Criterion sampling is used for sample selection. Consequently, 37 participants (20 for FGD and 17 for DI) from India were recruited.

Findings
The study’s findings indicate six broad attributes/factors (purchase attributes, product attributes, credibility, reviews/support, refurbishment process and ecological factors) that influence young consumers’ WTP refurbished products. In each attribute, some characteristics are unique (for example, greater trust in online platforms/stores, preference for video reviews and historical information of the product) to young consumers.

Originality/value
The current study integrates three literature streams: consumer behaviour in the online/offline context, young consumers’ inclination towards an online purchase and WTP refurbished products. To the best of the authors’ knowledge, this is the first study to do it.
Multiple parasitoid species enhance top‐down control, but parasitoid performance is context dependent
Authors: Thierry Mélanie , Pardikes Nicholas A. , Ximénez‐Embún Miguel G. , Proudhom Grégoire , Hrček Jan 
Published: July 2022

Abstract

Ecological communities are composed of many species, forming complex networks of interactions. Current environmental changes are altering the structure and species composition of ecological networks, which could modify interactions, either directly or indirectly. To predict changes in the functioning of communities, we need to understand whether species interactions are primarily driven by network structure (i.e. topology) or the specific identities of species (i.e. nodes). Yet, this partitioning of effects is challenging and thus rarely explored.

Here we disentangled the influence of network structure and the identities of species on the outcome of consumer–resource interactions using a host–parasitoid system.

We used four common community modules in host–parasitoid communities to represent network structure (i.e. host–parasitoid, exploitative competition, alternative host and a combination of exploitative competition and alternative host). We assembled nine different species combinations per community module in a laboratory experiment using a pool of three Drosophila hosts and three larval parasitoid species (Leptopilina sp., Ganaspis sp. and Asobara sp.). We compared host suppression and parasitoid performance across community modules and species assemblages to identify general effects linked to network structure and specific effects due to species community composition.

We found that multiple parasitoid species enhanced host suppression due to sampling effect, weaker interspecific than intraspecific competition between parasitoids, and synergism. However, the effects of network structure on parasitoid performance were species specific and dependent on the identity of co‐occurring species. Consequently, multiple parasitoid species generally strengthen top‐down control, but the performance of the parasitoids depends on the identity of either the co‐occurring parasitoid species, the alternative host species or both.

Our results highlight the importance of preserving parasitoid diversity for ecosystem functioning and show that other effects depend on species community composition, and may therefore be altered by ongoing environmental changes.