bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic criteria of small sellar lesions with hyperprolactinemia: Prolactinoma or else
Authors: Cho Anna , Vila Greisa , Marik Wolfgang , Klotz Sigrid , Wolfsberger Stefan , Micko Alexander 
Published: September 2022

ObjectiveTo evaluate the combined predictive value of MRI criteria with the prolactin-volume-ratio (PVR) to differentiate prolactinoma from non-prolactinoma, in small sellar lesions with hyperprolactinemia.MethodsRetrospective analysis of 55 patients with sellar lesions of ≤15 mm diameter on MRI and hyperprolactinemia of ≤150 ng/mL, surgically treated between 2003 and 2020 at the Medical University of Vienna, with a conclusive histopathological report. Serum prolactin levels, extent of pituitary stalk deviation, size and volume of the lesion were assessed. The PVR was calculated by dividing the preoperative prolactin level by tumor volume.ResultsOur study population consisted of 39 patients (71%) with a prolactin-producing pituitary adenoma (group A), while 16 patients (29%) had another type of sellar lesion (group B). Patients in group A were significantly younger (p=0.012), had significantly higher prolactin levels at diagnosis (p&lt;0.001) as well as smaller tumor volume (p=0.036) and lower degree of pituitary stalk deviation (p=0.009). The median PVR was significantly higher in group A (243 ng/mL per cm3) than in group B (83 ng/mL per cm3; p=0.002). Furthermore, the regression operating characteristics analysis revealed a PVR &gt;100 ng/mL per cm3 to be predictive for distinguishing prolactin-producing lesions from other small sellar lesions.ConclusionIn patients with small sellar lesions, Prolactin-Volume-Ratios &gt;100 represents a possible predictive marker for the diagnosis of prolactin-producing pituitary adenomas.
Application of Stranded Pelagic Sargassum Biomass as Compost for Seedling Production in the Context of Mangrove Restoration
Authors: Trench Camilo , Thomas Shanna-Lee , Thorney Delroy , Maddix Gina-Marie , Francis Patrice , Small Hugh , Machado Carla Botelho , Webber Dale , Tonon Thierry , Webber Mona 
Published: September 2022

The Sargassum spp. inundations across the Atlantic and Caribbean that began in 2011 have continued unabated, and new uses for the biomass are being continuously explored. Mangroves protect shorelines, store carbon, enhance water quality, and promote biodiversity. Their restoration can be hindered by poor soils associated with urbanized coastlines. Sargassum spp. application in the form of mulch, compost, and plant tonics has yielded positive results in a range of plants. As part of transforming the inundations to benefit communities, Sargassum spp. compost (SC) was assessed in mangrove seedling production for restoration. Pure SC was mixed with soil/sand medium, as different treatments, for the production of Rhizophora mangle seedlings in “wet” and “dry” nurseries. Plants in the “wet” nursery performed poorly, with 90–100% of plants in 50 and 100% SC, respectively, dying after 6 weeks. Seedlings in all SC treatments in the “dry” nursery survived with obvious and statistically significant treatment differences. Height and number of leaves indicated the best growth in the 75% SC treatment, while the control (0% SC) had the poorest growth. Seedling health, greatest in the control, was poorest in 50 and 100% SC. Elemental analysis of SC, seedlings, and soil/sand medium indicated that several elements (Na, K, Ca, As, and Se) found in high concentrations in the SC were low in the plants. Overall, low sequestration of elements by mangrove seedlings and the reported ability of mangrove soils to reduce element mobilization through chelation indicate the potential use of Sargassum spp. in soil amelioration for mangrove restoration without proportional contamination of the ecosystem. We see the potential use of nuisance Sargassum spp. blooms to support mangrove restoration, leading to increased benefits to coastal communities being affected by the inundations.
Soil quality restoration and yield stabilization in acidic soils of northeastern Himalayas: Five years impact of green manuring and crop residue management
Authors: Ansari Meraj A. , Babu Subhash , Choudhary Jairam , Ravisankar Natesan , Panwar Azad S. 
Published: September 2022

Soil quality restoration and crop productivity maximization are the global challenge to feed the galloping population. The task is much more daunting in a risk-prone, fragile, and low productive hilly region due to the depletion of supporting and regulating ecosystem services. A five-year long-term (2012–2017) field experiment was conducted to stabilize the yield and soil quality through legume green manuring and crop residue recycling in intensified cropping systems in the Eastern Himalayan region of India. Four treatments involving three green manures [green gram (Vigna radiata); cowpea (Vigna unguiculata);Sesbania(Sesbania aculeata) along with control (no-green manure)], three cropping systems [groundnut (Arachis hypogaea)—pea (Pisum sativum); maize (Zea mays)—pea, and maize + groundnut–pea] and two levels of residue management practices [residue removal and residue retention] were evaluated in three times replicated split–split plot design. Among the green manure options,Sesbaniaexerted a significant positive impact on the soil organic carbon (SOC) stock, available micro- (Fe, Mn, Zn, and Cu), and macronutrients (N, P and K) in surface (0–0.15 m) and subsurface (0.15–0.45 m) soils. The improvement in soil enzymatic activities (acid phosphatase, alkaline phosphatase, dehydrogenase, beta-glucosidase, and aryl sulfatase activity) (p&lt; 0.05) inSesbania-treated soil was +28.1% to +38.9% in surface and +18.3% to +27.3% in subsurface soils over non-green manure.Sesbania-treated soils also exhibited higher soil quality index (SQI) and stratification ratio (SR) of available soil nutrients and enzymes over non-green manured soils. Among the cropping systems, groundnut intercropped with maize followed by peas (MGP) within situresidue retention increased (p&lt; 0.05) the available soil macro- and micronutrients, SOC stock, soil enzymes, SR, and SQI in comparison to other cropping systems.Sesbaniagreen manuring and residue retention improved the yield sustainability by +19% and +11% in the MGP system over non-green manuring and residue removal, respectively. Therefore,Sesbaniagreen manuring in the MGP cropping system along with residue retention is recommended for stabilizing the soil quality through enhancing supporting and regulating ecosystem services and maintaining long-term productivity in the fragile Eastern Himalayan ecosystem of India.
Determinants of villagers’ satisfaction with post-disaster reconstruction: Evidence from surveys ten years after the Wenchuan earthquake
Authors: Ao Yibin , Zhong Jinglin , Zhang Zijun , Han Lili , Wang Yan , Chen Yunfeng , Wang Tong 
Published: September 2022

Residents’ satisfaction with post-disaster reconstruction in earthquake-stricken areas directly affects their quality of life, which cannot be ignored in post-disaster reconstruction. More than 10 years after the Wenchuan earthquake, we took ten randomly selected villages in the five areas hardest-hit by the Wenchuan earthquake as research objects and obtained 483 valid completed questionnaires. The villagers were randomly sampled and descriptive statistical analysis, factor analysis, and ordered logistic regression were used to explore the factors and relationships influencing villagers’ satisfaction with post-disaster reconstruction in Wenchuan earthquake-stricken areas. The results show that: 1) the more rural residents know about the post-disaster reconstruction, the greater their level of satisfaction; 2) the more the annual income of families increases after resettlement, the greater the satisfaction of rural residents with the post-disaster reconstruction; 3) six public factors, namely the village committee acts as, housing construction quality, public service, policy of benefiting farmers, cultural environment, and hardware environment, all significantly positively affect residents’ overall satisfaction with post-earthquake reconstruction. This study thus enriches the theory of residents’ satisfaction studies and the practice of post-earthquake reconstruction.
A novel immune-related radioresistant lncRNAs signature based model for risk stratification and prognosis prediction in esophageal squamous cell carcinoma
Authors: Zheng Jianqing , Chen Xiaohui , Huang Bifen , Li Jiancheng 
Published: September 2022

Background and purpose: Radioresistance remains a major reason of radiotherapeutic failure in esophageal squamous cell carcinoma (ESCC). Our study is to screen the immune-related long non-coding RNA (ir-lncRNAs) of radiation-resistant ESCC (rr-ESCC) via Gene Expression Omnibus (GEO) database and to construct a prognostic risk model.Methods: Microarray data (GSE45670) related to radioresistance of ESCC was downloaded from GEO. Based on pathologic responses after chemoradiotherapy, patients were divided into a non-responder (17 samples) and responder group (11 samples), and the difference in expression profiles of ir-lncRNAs were compared therein. Ir-lncRNA pairs were constructed for the differentially expressed lncRNAs as prognostic variables, and the microarray dataset (GSE53625) was downloaded from GEO to verify the effect of ir-lncRNA pairs on the long-term survival of ESCC. After modelling, patients are divided into high- and low-risk groups according to prognostic risk scores, and the outcomes were compared within groups based on the COX proportional hazards model. The different expression of ir-lncRNAs were validated using ECA 109 and ECA 109R cell lines via RT-qPCR.Results: 26 ir-lncRNA genes were screened in the GSE45670 dataset with differential expression, and 180 ir-lncRNA pairs were constructed. After matching with ir-lncRNA pairs constructed by GSE53625, six ir-lncRNA pairs had a significant impact on the prognosis of ESCC from univariate analysis model, of which three ir-lncRNA pairs were significantly associated with prognosis in multivariate COX analysis. These three lncRNA pairs were used as prognostic indicators to construct a prognostic risk model, and the predicted risk scores were calculated. With a median value of 2.371, the patients were divided into two groups. The overall survival (OS) in the high-risk group was significantly worse than that in the low-risk group (p &lt; 0.001). The 1-, 2-, and 3-year prediction performance of this risk-model was 0.666, 0.702, and 0.686, respectively. In the validation setting, three ir-lncRNAs were significantly up-regulated, while two ir-lncRNAs were obviouly down-regulated in the responder group.Conclusion: Ir-lncRNAs may be involved in the biological regulation of radioresistance in patients with ESCC; and the prognostic risk-model, established by three ir-lncRNAs pairs has important clinical value in predicting the prognosis of patients with rr-ESCC.
Unlocking phenotypic plasticity provides novel insights for immunity and personalized therapy in lung adenocarcinoma
Authors: Wang Feng , Du Hongjuan , Li Bibo , Luo Zhibin , Zhu Lei 
Published: September 2022

Background: Unlocking phenotype plasticity (UPP) has been shown to have an essential role in the mechanism of tumor development and therapeutic response. However, the clinical significance of unlocking phenotypic plasticity in patients with lung adenocarcinoma is unclear. This study aimed to explore the roles of unlocking phenotypic plasticity in immune status, prognosis, and treatment in patients with lung adenocarcinoma (LUAD).Methods: Differentially expressed genes (DEGs) and clinical information of UPP were selected from the cancer genome atlas (TCGA) database, and the GO, KEGG enrichment analyses were performed. The independent prognostic genes were determined by univariate and multivariate Cox regression, and the UPP signature score was constructed. Patients with LUAD were divided into high- and low-risk groups according to the median of score, and the immunocytes and immune function, the gene mutation, and drug sensitivities between the two groups were analyzed. Finally, the results were validated in the GEO database.Results: Thirty-nine significantly DEGs were determined. Enrichment analysis showed that UPP-related genes were related to protein polysaccharides and drug resistance. The prognostic results showed that the survival of patients in the high-risk group was poorer than that in the low-risk group (p &lt; 0.001). In the high- and low-risk groups, single nucleotide polymorphism (SNP) and C &gt; T are the most common dissent mutations. The contents of immune cells were significantly different between high- and low-risk groups. And the immune functions were also significantly different, indicating that UPP affects the immunity in LUAD. The results from TCGA were validated in the GEO.Conclusion: Our research has proposed a new and reliable prognosis indicator to predict the overall survival. Evaluation of the UPP could help the clinician to predict therapeutic responses and make individualized treatment plans in patients with LUAD.
Comprehensive multiomics analysis of cuproptosis-related gene characteristics in hepatocellular carcinoma
Authors: Fu Jie , Wang Sixue , Li Zhenghao , Qin Wei , Tong Qing , Liu Chun , Wang Zicheng , Liu Zhiqiang , Xu Xundi 
Published: September 2022

Background: The mechanism of copper-induced cell death, which is called cuproptosis, has recently been clarified. However, the integrated role of cuproptosis-related genes in hepatocellular carcinoma (HCC) and its relationship with immune characteristics are still completely unknown.Methods: In this study, the expression, genetic, and transcriptional regulation states of 16 cuproptosis-related genes in HCC were systematically investigated. An unsupervised clustering method was used to identify distinct expression patterns in 370 HCC patients from the TCGA-HCC cohort. Differences in functional characteristics among different expression clusters were clarified by gene set variation analysis (GSVA). The abundances of immune cells in each HCC sample were calculated by the CIBERSORT algorithm. Next, a cuproptosis-related risk score was established based on the significant differentially expressed genes (DEGs) among different expression clusters.Results: A specific cluster of HCC patients with poor prognosis, an inhibitory immune microenvironment, and high expression levels of immune checkpoint molecules was identified based on the expression of the 16 cuproptosis-related genes. This cluster of patients could be well-identified by a cuproptosis-related risk score system. The prognostic value of this risk score was validated in the training and two validation cohorts (TCGA-HCC, China-HCC, and Japan-HCC cohorts). Moreover, the overall expression status of the cuproptosis-related genes and the genes used to establish the cuproptosis-related risk score in specific cell types of the tumor microenvironment were preliminarily clarified by single-cell RNA (scRNA) sequencing data.Conclusion: These results indicated that cuproptosis-related genes play an important role in HCC, and targeting these genes may ameliorate the inhibitory immune microenvironment to improve the efficacy of immunotherapy with immune checkpoint inhibitors (ICIs).
COVID-19–associated lncRNAs as predictors of survival in uterine corpus endometrial carcinoma: A prognostic model
Authors: Ding Yang , Li Xia , Li Jiena , Liqun Zhu  
Published: September 2022

Background: Patients with uterine corpus endometrial carcinoma (UCEC) may be susceptible to the coronavirus disease-2019 (COVID-19). Long non–coding RNAs take on a critical significance in UCEC occurrence, development, and prognosis. Accordingly, this study aimed to develop a novel model related to COVID-19–related lncRNAs for optimizing the prognosis of endometrial carcinoma.Methods: The samples of endometrial carcinoma patients and the relevant clinical data were acquired in the Carcinoma Genome Atlas (TCGA) database. COVID-19–related lncRNAs were analyzed and obtained by coexpression. Univariate, least absolute shrinkage and selection operator (LASSO), and multivariate Cox regression analyses were performed to establish a COVID-19–related lncRNA risk model. Kaplan–Meier analysis, principal component analysis (PCA), and functional enrichment annotation were used to analyze the risk model. Finally, the potential immunotherapeutic signatures and drug sensitivity prediction targeting this model were also discussed.Results: The risk model comprising 10 COVID-19–associated lncRNAs was identified as a predictive ability for overall survival (OS) in UCEC patients. PCA analysis confirmed a reliable clustering ability of the risk model. By regrouping the patients with this model, different clinic-pathological characteristics, immunotherapeutic response, and chemotherapeutics sensitivity were also observed in different groups.Conclusion: This risk model was developed based on COVID-19–associated lncRNAs which would be conducive to the precise treatment of patients with UCEC.
Understanding gender dynamics in mHealth interventions can enhance the sustainability of benefits of digital technology for maternal healthcare in rural Nigeria
Authors: Udenigwe Ogochukwu , Okonofua Friday E. , Ntoimo Lorretta F. C. , Yaya Sanni 
Published: September 2022

IntroductionNigeria faces enormous challenges to meet the growing demands for maternal healthcare. This has necessitated the need for digital technologies such as mobile health, to supplement existing maternal healthcare services. However, mobile health programs are tempered with gender blind spots that continue to push women and girls to the margins of society. Failure to address underlying gender inequalities and unintended consequences of mobile health programs limits its benefits and ultimately its sustainability. The importance of understanding existing gender dynamics in mobile health interventions for maternal health cannot be overstated.ObjectiveThis study explores the gender dimensions of Text4Life, a mobile health intervention for maternal healthcare in Edo State, Nigeria by capturing the unique perspectives of women who are the primary beneficiaries, their spouses who are all men, and community leaders who oversaw the implementation and delivery of the intervention.MethodThis qualitative study used criterion-based purposive sampling to recruit a total of 66 participants: 39 women, 25 men, and two ward development committee chairpersons. Data collection involved 8 age and sex desegregated focus group discussions with women and men and in-depth interviews with ward development committee chairpersons in English or Pidgin English. Translated and transcribed data were exported to NVivo 1.6 and data analysis followed a conventional approach to thematic analysis.ResultsWomen had some of the necessary resources to participate in the Text4Life program, but they were generally insufficient thereby derailing their participation. The program enhanced women's status and decision-making capacity but with men positioned as heads of households and major decision-makers in maternal healthcare, there remained the possibility of deprioritizing maternal healthcare. Finally, while Text4Life prioritized women's safety in various contexts, it entrenched systems of power that allow men's control over women's reproductive lives.ConclusionAs communities across sub-Saharan Africa continue to leverage the use of mHealth for maternal health, this study provides insights into the gender implications of women's use of mHealth technologies. While mHealth programs are helpful to women in many ways, they are not enough on their own to undo entrenched systems of power through which men control women's access to resources and their reproductive and social lives.
Serial infection with SARS-CoV-2 Omicron BA.1 and BA.2 following three-dose COVID-19 vaccination
Authors: Lapointe Hope R. , Mwimanzi Francis , Cheung Peter K. , Sang Yurou , Yaseen Fatima , Kalikawe Rebecca , Datwani Sneha , Waterworth Rachel , Umviligihozo Gisele , Ennis Siobhan , Young Landon , Dong Winnie , Kirkby Don , Burns Laura , Leung Victor , Holmes Daniel T. , DeMarco Mari L. , Simons Janet , Matic Nancy , Montaner Julio S.G. , Brumme Chanson J. , Prystajecky Natalie , Niikura Masahiro , Lowe Christopher F. , Romney Marc G. , Brockman Mark A. , Brumme Zabrina L. 
Published: September 2022

SARS-CoV-2 Omicron infections are common among individuals who are vaccinated or have recovered from prior variant infection, but few reports have immunologically assessed serial Omicron infections. We characterized SARS-CoV-2 humoral responses in an individual who acquired laboratory-confirmed Omicron BA.1.15 ten weeks after a third dose of BNT162b2, and BA.2 thirteen weeks later. Responses were compared to 124 COVID-19-naive vaccinees. One month post-second and -third vaccine doses, the participant’s wild-type and BA.1-specific IgG, ACE2-displacement and virus neutralization activities were average for a COVID-19-naive triple-vaccinated individual. BA.1 infection boosted the participant’s responses to the cohort ≥95th percentile, but even this strong “hybrid” immunity failed to protect against BA.2. Reinfection increased BA.1 and BA.2-specific responses only modestly. Though vaccines clearly protect against severe disease, results highlight the continued importance of maintaining additional protective measures to counteract the immune-evasive Omicron variant, particularly as vaccine-induced immune responses naturally decline over time.
Spatio-temporal analysis of east greenland polar front
Authors: Liu Yu , Wang Jianhui , Han Guoqing , Lin Xiayan , Yang Guijing , Ji Qiyan 
Published: September 2022

The East Greenland Polar Front (EGPF) is an important front with strong salinity and temperature gradients in the Nordic Seas. It is formed by the interaction between Arctic-origin and Atlantic-origin water. The variations of EGPF are closely linked with sea ice melting and heat content transport associated with North Atlantic water recirculation. For a three-dimensional (3D) daily analysis, we use the global ocean eddy resolution reanalysis product (GLORYS12V1) from 1993 to 2018 to calculate the salinity and temperature horizontal gradient in the upper ocean and obtain the spatiotemporal distribution and intensity characteristics of EGPF. After assessment, the thresholds of the salinity and temperature fronts are set to 0.04 psu/km and 0.09°C/km, respectively. Compared with satellite observations of sea ice concentration, a significant spatial relationship is observed between the main position of EGPF and the ice edge before the sea ice shrinks to the continental shelf sea area. Affected by the freshening of the Arctic-origin water due to the melting of the sea ice, the intensity and area of EGPF show significant seasonal variations. Against the background of global warming, the sea ice area presents an obvious decreasing trend in the Greenland Sea. The melting of sea ice increased annually every summer from 1998 to 2018. The heat content transport of the Atlantic-origin water has also increased in recent years. The 3D characteristics (intensity and volume) of EGPF as salinity and temperature fronts exhibit increasing trends.