bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogrouping and pathogrouping of Potato virus Y isolates from pepper plants in Turkey
Authors: TOPKAYA Serife , Gibbs Adrian J 
Published: September 2022

Abstract
Potato virus Y(PVY) causes yield loss of pepper (Capsicum) crops worldwide, but little is known of its molecular diversity in pepper in Turkey or elsewhere. During 2016 and 2017, pepper crops were surveyed in three different provinces of Turkey, and plants with virus-like symptoms were collected. Four hundred and twenty one samples were collected and 35 found to be infected with PVY by DAS-ELISA using PVY-specific antisera. The positive samples were tested by RT-PCR and viral genes of some were sequenced. Two schemes, and mixtures thereof, are currently used for grouping and naming isolates of PVY. Eight of the pepper isolates were probably from phylogroup C, and three from pathogroup N-Wi, which is part of recombinant phylogroup R1. We discuss the theory and practice of grouping PVY isolates. This is the first report of recombinant PVY isolates in pepper plants in Turkey.
The contribution of cell hashing for single-cell analysis of acute leukemia
Authors: Madaci Lamia , Gard Charlyne , Nin Sébastien , Venton Geoffroy , Rihet Pascal , Puthier Denis , Loriod Béatrice , Costello Régis 
Published: September 2022

Abstract
After decades during which the treatment of acute myeloblastic leukemia consisted in cytarabine + anthracycline, targeted therapies have appeared, first based on monoclonal antibodies (anti-CD52, anti-CD123) and then on specific inhibitors of molecular mutations (anti-IDH, IDH2 or FLT3). What should be the place of these therapeutic options considering the tumor heterogeneity inherent to leukemia diagnosis and the clonal drift of which this type of tumor is capable? Targeted drugs would require an analysis of the various therapeutic targets not in the total population but at the individual cell level. Indeed, the prognostic value and therapeutic interest of a given molecular target are certainly not the same if it is a cell in terminal differentiation with low proliferative potential or, on the contrary, a stem cell with strong capacities of both proliferation and self-renewal. However, this cell-by-cell analysis is fraught with several pitfalls. The first one is scientific because the comparison of two different single cell analysis experiments is delicate, in spite of the different techniques aiming at standardizing the results. The second pitfall is practical, as each single cell experiment is very costly from a financial point of view but also very time consuming. The solution is therefore to be able to process several samples at the same time, which is the specificity of the cell hashing technique. In this study we demonstrate that the cell hashing technique can be used for the analysis of acute myeloid leukemia cells. We compared the cell hashing technique with the classic single cell analysis and demonstrated a good concordance of different parameters: quality control, gene expression correlation, expression analysis of leukemic blast markers in both patients. The technique could thus in the future be part of the biological assessment of acute myeloid leukemia and contribute to the individualization and optimization of their management, particularly in the context of the use of targeted therapies.
Differential Evolution Algorithms with Novel Mutations, Adaptive Parameters and Weibull Flight Operator
Authors: Layeb Abdesslem 
Published: September 2022

Abstract
Differential evolution (DE) is among the best evolutionary algorithms for global optimization. However, the basic DE has several shortcomings like the slow convergence speed and it is more likely to be stuck at a local optimum. Moreover, the performance of DE is sensitive to its mutation strategies and mutation and crossover control parameters. In this scope, we present in this paper three mechanisms to overcome DE limitations. Firstly, two novel mutations called DE/mean-current/1 and DE/best-mean-current/1 are proposed and integrated in DE algorithm, they have both exploration ability and exploitation trend. On the other hand, to avoid to be trapped in local minima of hard functions, a new exploration operator has proposed called Weibull flight based on the Weibull distribution. Finally, new adapted control parameters based also on the Weibull distribution are integrated. The efficacy of the proposed algorithms called meanDE, MDEW, AMDE and AMDEW is validated throughout intensive experimentations using classical tests, some challenging tests, the CEC2017, CEC2020, the most recent CEC2022, four constraint engineering problems, and data clustering problem. Moreover, comparison with several popular, recent, and high-performance optimization algorithms showed a high effectiveness of the proposed algorithms in locating the optimal or near-optimal solutions with higher efficiency. The efficiency of the new mutations is clear compared to the standard DE mutations. Moreover, the proposed Weibull flight has great capacity to deal with hard composition functions of CEC benchmarks. Finally, the use of the adapted control parameter of mutation scale helps to avoid the parameters setting problem present in several metaheuristics.
Design and Dynamic Modeling of a Coupled-Drive Flexible Joint for Nursing-Care Robot Arm
Authors: Lu Hao , Guo Shi-Jie , Yang Zhi-Qiang , Deng Fei , Wang Hong-Bo 
Published: September 2022

Abstract
Patient transfer is one of the most physically challenging tasks in nursing care, the burden of which can be reduced by introducing nursing-care robots. This study proposes a coupled-drive 2-DOF (degree of freedom) joint for nursing-care robot arms to ensure that the arms have a high load capacity and smooth configuration, which is crucial for safe and comfortable contact with the care receiver. This paper presents the design methodology of the coupled-drive joint by using differential mechanism which can superimposing the torque of two motors to achieve a high load capacity. To make the joint flexible for this purpose, we introduced rubber layers in the connections of the major parts. We expressed the dynamics of the rubber layers using a hyper-viscoelastic model and applied it to the joint dynamics to build a dynamic model of the coupled-drive 2-DOF joint using the Lagrange method. To demonstrate the effectiveness of the arms with coupled-drive joints in patient transfer tasks, we built two arms, integrated them into a robot, and conducted a transfer experiment in which the motions were planned based on the dynamic model. The results showed that the designed joints had both a high load capacity and flexibility for safe and comfortable contact with humans.
Influencing factors of hyperuricemia and gene analysis of familial gout among the elderly in Shijing Town, Nan'an City, Fujian Province
Authors: Tam HoYin , Zhang ShiTing , Su HaoBin , Yin XiaoJian , Wang TianYi , Zhao Hong , Cheng JiDong 
Published: September 2022

Abstract
Background
To investigate and analyze the relationship between hyperuricemia and various metabolic diseases and complications in people over 60 years old in Shijing Town, Nan'an City, Fujian Province, as well as the genetic analysis of familial gout attacks.
Methods
A cross-sectional study was conducted on the health examination data of people over 60 years old in Shijing Town, Nan'an City, Fujian Province (total population of 78,500) in 2019 and 2020. The differences between groups of metabolic indicators and complications and the correlation between uric acid and various metabolic indicators were analyzed. And 2 gout attack families were selected and used clinical whole-exome sequencing (including mitochondria) to conduct genome-wide association analysis.
Results
The total prevalence rate of hyperuricemia was 29.04%; albumin, triglyceride, alanine aminotransferase, aspartate aminotransferase, potassium ion, blood urea nitrogen, blood glucose, creatinine, body mass index, hypertension, and fatty liver were higher than those in the non-hyperuricemia group, and the difference was statistically significant (P < 0.05); the high-density lipoprotein in the hyperuricemia group was lower than that in the non-hyperuricemia group blood group, the difference was statistically significant (P < 0.05). There was no significant difference in serum total cholesterol, low-density lipoprotein, total bilirubin, direct bilirubin, sodium ion, diabetes, coronary heart disease, and abnormal electrocardiogram (P > 0.05); serum uric acid level in people over 60 years old It was positively correlated with albumin, triglyceride, low-density lipoprotein, potassium ion, alanine aminotransferase, aspartate aminotransferase, and body mass index levels (P < 0.05), and negatively correlated with HDL level (P < 0.05). P < 0.05); each of SLC4A1 gene, ABCB4 gene, and LRBA gene mutation was detected in the two tested families, and no mutation of SLC2A9 and ABCG2 gene was found.
Conclusions
The prevalence of hyperuricemia in the elderly population in Nan'an City, Fujian Province (29.04%) is significantly higher than the average level of the elderly in my country (13.1%). Compared with the normal population, patients have significantly abnormal metabolism. There is a significant correlation with the abnormal degree of familial hyperuricemia. Hypertension and fatty liver are risk factors for hyperuricemia; the SLC4A1 gene, ABCB4 gene, and LRBA gene may be closely related to the occurrence of familial hyperuricemia.
Association of maternal main pulmonary arterial diameter and neonatal birth weight: a study based on pulmonary arterial hypertension
Authors: Zhao Kaixun , Zhang Caojin , Yang Ziyang , Zhou Yin , Xie Nanshan , Lai Disheng 
Published: September 2022

Abstract
OBJECTIVE
To study the association of maternal main pulmonary arterial diameter and neonatal weight of pregnant women with pulmonary arterial hypertension.
METHODS
We divide patients into 2 group, according to whether the patients with pulmonary arterial hypertension owing to congenital heart disease, or idiopathic pulmonary arterial hypertension. Univariate and multivariate regression analysis were used. After adjusting for various confounding, tangent points were obtained by fitting curves and threshold effect analysis. Finally, we apply a stratified analysis according to New York Heart Association (NYHA) Functional Classification.
RESULTS
According to the results of multivariate regression analysis, in patients with PAH owing to congenital heart disease, maternal pulmonary arterial diameter was significantly associated with neonatal birth weight, and the regression coefficient was − 37.03, and the 95% confidence interval was − 63.44 to -10.62, P value < 0.001. The result of regression analysis is that when maternal main pulmonary arterial diameter increases by 1 mm, the weight of the newborn will decrease by 37.03g. After stratified analysis, the regression coefficient was − 40.94, and the 95% confidence interval was − 69.68 to -12.20, P value < 0.001.
CONCLUSION
The maternal main pulmonary artery diameter was significantly correlated with the neonatal birth weight in patients with pulmonary arterial hypertension owing to congenital heart diseases, especially thoses with functional classification I or II.
Tea consumption and Papillary Thyroid Cancer Risk: a Case-Control Study of Adults in Shanghai, China
Authors: Xu Xiaoli , Zang Jiajie , Wang Zhengyuan , Wang Jiadong , Wu Yi , Liu Meixia , Shi Zehuan , Song Qi , Cui Xueying , Wu Fan 
Published: September 2022

Abstract
Few studies have explored the impact of consumption of three types of tea on the risk for papillary thyroid cancer (PTC) in East Asia. A case-control study was conducted with323 Chinese patients with histological confirmed differentiated thyroid cancer and 323 healthy subjects selected from the same hospital. Participants were matched by age, nationality and gender, and interviewed face-to-face by trained interviewers using a structured questionnaire. Participants who drank tea at least once a day (p = 0.023) or consumed ≤ five grams each time (p = 0.047) had a lower PTC risk than unfermented tea non-drinkers had. Participants who drank tea at least once a month but < once a day (p = 0.023) or consumed ≤ five grams each time (p = 0.018) had a lower PTC risk than did semi-fermented tea non-drinkers. Among females, a significantly lower PTC risk was found in those who drank unfermented tea at least once a day (p = 0.030) compared with the unfermented tea non-drinkers. No dose-response relationship of the ever-drinkers’ three tea types was found between the amount of tea consumed each time and a lower PTC risk among all participants. Consuming unfermented tea at least once a day was the most protective factor in lowering PTC risk among females.
A Distributed Algorithm for Solving Large-scale p-median Problems Using Expectation Maximization
Authors: Gwalani Harsha , Helsing Joseph , Alshammari Sultanah , Tiwari Chetan , Mikler Armin 
Published: September 2022

Abstract
The p-median problem selects p source locations to serve n destinations such that the average distance between the destinations and corresponding sources is minimized. It is a well-studied NP-hard combinatorial optimization problem with many existing heuristic solutions, however, existing algorithms are not scalable for large-scale problems. The Fast Interchange (FI) heuristic which yields results close to the optimal solution with respect to the objective function value becomes suboptimal with respect to time requirements for large-scale problems. We present a novel distributed multi-core divide and conquer algorithm, EM-FI, to solve large-scale p-median problems quickly even with limited computing resources. The algorithm identifies the existing spatial clusters of the destination locations using Expectation Maximization(EM) and solves them as independent p-median problems using Integer Programming or FI concurrently. The proposed algorithm showed an order of magnitude improvement in time without the loss of quality in terms of the objective function value on synthetic and real datasets.
Primary sarcoma of the uterine cervix: Experience of a single center
Authors: Yuan Hua , Li Ning 
Published: September 2022

Abstract
Objectives To investigate the clinicopathological characteristics and prognosis of patients diagnosed with primary sarcoma of the uterine cervix.
Methods We identified all patients with primary cervical sarcomas treated at our institution from 2002 to 2020. Clinical and pathologic variables, treatment modalities, and prognosis were abstracted from the medical records.
Results Thirty-four patients were identified. Of these patients, 7 (20.6%) patients had leiomyosarcoma, 6 (17.6%) patients had carcinosarcoma or malignant mixed Müllerian tumor, 5 ( 14.7% ) patients had Ewing's sarcoma, 4 (11.8%) patients had rhabdomyosarcoma , 4 (11.8%) patients had undifferentiated sarcoma, 3 (8.8%) patients had sarcoma not otherwise specified, 2 (5.9%) patients had adenosarcoma, 2 (5.9%) patients had endometrial stromal sarcoma, and 1 (2.9%) patient had alveolar soft tissue sarcoma. The median age of the whole patients was 43.5 years (range, 13-63). The median age of patients with Ewing's sarcoma or rhabdomyosarcoma was 22 years (range, 13-39) and 17 years (range, 13-36), respectively. Twenty-four patients presented with vaginal bleeding which was the most common primary symptoms. The 2018 distribution by stage was: stage I in 21 (61.8%) patients, stage II in 4 (11.8%) patients, stage III in 6 (17.6%) patients and stage IV in 3 (8.8%) patients.
Overall, 30 patients (88.2%) received surgical treatment and 4 patients (11.8%) with advanced disease received systematic chemotherapy with or without radiotherapy. The median follow-up was 33.3 months (range 3.6–187.3 months). 15 patients died during follow up, and 11 patients died within 2 years after diagnosis, most of them were patients with carcinosarcoma or undifferentiated sarcoma (45.5%, 5/11). In the entire cohort, 2-year overall survival (OS) and 5-year OS were 67.2% and 56.9%, respectively. For patients with different pathologic subtype, 5-year OS was 25.0% for undifferentiated sarcoma, 50.0% for rhabdomyosarcoma, 50.0% for carcinosarcoma, 53.3% for Ewing's sarcoma, 57.1% for leiomyosarcoma, and 75.0% for others. The 5-year OS for stage I-II and III-IV patients were 62.3% and 41.7%, respectively.
Conclusions Cervical sarcomas are rare neoplasms with multiple histological subtypes and follow an aggressive course. Surgery is the main treatment for cervical sarcoma. Prognosis may be associated with tumor histology and stage.
GRHPR, targeted by miR-138-5p, inhibits the proliferation and metastasis of hepatocellular carcinoma through PI3K/AKT signaling pathway
Authors: Yang Shuangshuang , Liu Yixian , Zhang Bushi , Li Jinxia , Xu Fang , Yu Mengdan , Chen Ying , Li Chenglong , Liu Ting , Zhao Ying , Zhao Qianwei , Zhang Jintao 
Published: September 2022

Abstract
Background
Hepatocellular carcinoma (HCC) is one of the most aggressive cancers. The present study aimed to investigate the effects of GRHPR on the proliferation and metastasis of HCC and to identify the miRNA targeting GRHPR to understand the underlying mechanism of miR-138-5p/GRHPR on HCC proliferation and metastasis.
Methods
The expression levels of GRHPR and miR-138-5p were detected in HCC tissues and cells. Bioinformatic analysis and dual-luciferase reporter assay were carried out to confirm that GRHPR was a target gene of miR-138-5p. HCC cell lines overexpressing GRHPR were established to detect the roles of GRHPR in HCC cell proliferation, migration and invasion. The biological function of miR-138-5p targeting to GRHPR in HCC cell lines was also evaluated. The xenograft mouse model was established to investigate the functions of GRHPR in vivo.
Results
GRHPR was markedly downregulated in human HCC samples, whereas miR-138-5p was upregulated in HCC samples. The expression of GRHPR was regulated by miR-138-5p in HCC cell lines. HCC cell proliferation, migration and invasion could be suppressed by GRHPR overexpression and GRHPR exerted an anti-tumor effect in a xenograft mouse model. However, the inhibition of GRHPR by miR-138-5p promoted the proliferation and invasive properties of HCC cells. MiR-138-5p could regulate the phosphorylation levels of PI3K and AKT related to the phosphatidylinositol signaling pathway via inhibiting GRHPR expression.
Conclusions
This study suggests that GRHPR may play a significant role in HCC and its function is regulated by miR-138-5p.
Presence and risk assessment of pharmaceutically active compounds in neotropical small basins, Brazil
Authors: Minillo Alessandro , Isique William , Cardoso Claudia , Súarez Yzel Rondon 
Published: September 2022

Abstract
Pharmaceutically active compounds (PAC) are micropollutants that represent emerging contaminants (ECs) commonly found in aquatic environments. Exposure of aquatic biota to these identified ECs has resulted in severe effects. In the last few years, the number of studies on PAC has grown quickly owing to increasing awareness of the potential environmental and health risks related to these micropollutants. The aim of our study was to evaluate the contamination levels of selected PAC and their potential ecological threat to forested streams. Samples of stream water were collected in the second largest city in Mato Grosso do Sul State in center-west of Brazil. Physicochemical parameters and concentrations of PAC were quantified in samples collected in six field campaigns. Ecological risk assessment (ERA) based on risk quotient (RQ) was carried out based on the maximum measured concentration of PAC in water. Six pharmaceutical compounds were successfully quantified in the forested streams, namely caffeine (1.0 – 1,696.04 ng/L), naproxen (0.9 – 681.80 ng/L), diclofenac (0.8 – 849.94 ng/L), estriol (0.9 – 13.22 ng/L), estradiol (0.9 – 587.30 ng/L) and ethinylestradiol (0.9 – 790.90 ng/L). From an ecological risk perspective, ethinylestradiol, estradiol, diclofenac and caffeine can be considered as priority PAC because they showed moderate to high risks to aquatic organisms and may further lead to damages along the food chain. This study provides valuable information to reinforce the importance of continuous monitoring of the forested streams in center-west region of the country, as well as efforts to control the entry of these micropollutants in watercourse.
Do Adult Patients with Congenital Colorectal Conditions know their Diagnosis?
Authors: Vargas Maria Camila , Wehrli Lea , Louiselle Amanda , Ketzer Jill , Reppucci Marina , Judd-Glossy Laura , Alaniz Veronica , Wilcox Duncan , Wood Dan , Peña Alberto , Torre Luis de la , Bischoff Andrea 
Published: September 2022

Abstract
Purpose
Limited research exists about the knowledge that adult patients have about their congenital colorectal diagnosis.
Methods
This was an IRB approved, prospective study of patients in the Adult Colorectal Research Registry who completed surveys between October 2019 and March 2022. Surveys were administered through REDCap after patients consented to being contacted for research purposes. Patients provided demographic data, which was linked to surgical records, and the diagnoses provided by patients were compared with diagnoses recorded by the original surgeons.
Results
One hundred and thirty-one questionnaires were collected, 115 patients had anorectal malformations (ARM) and 16 had Hirschsprung disease (HD). Seven patients who had ARM were unaware that they had an ARM or HD. The type of ARM recorded by the surgeon was unavailable for comparison with the patient's reported diagnosis in four cases. Of the 111 remaining patients with ARM, only 32 of them (29%) knew what their own type of anomaly was. Female patients recalled their diagnosis more often than male patients (42.4% versus 13.5%). All 16 participants with HD correctly identified their diagnosis severity as HD with or without Total Colonic Aganglionosis.
Conclusion
The results of this study demonstrate patients’ limited understanding of their type of ARM and highlight the urgent need to enhance communication and education strategies, such as issuing patients with medical diagnosis identification cards. It is critical for clinicians to better communicate with patients to ensure that they and their relatives truly understand their precise diagnosis. Better informed patients should be better able to advocate for themselves, adhere to treatments and precautionary recommendations, navigate the complexities of transitional care, and more effectively manage lifelong complications.
Non-isothermal thin-film flow of a viscoplastic material over topography
Authors: Moyers-Gonzalez Miguel , Hewett James N. , Cusack Dale R. , Kennedy Ben M. , Sellier Mathieu 
Published: September 2022

Abstract
This paper considers the non-isothermal flow of a viscoplastic fluid on a horizontal or an inclined surface with a flat, a step-up and a step-down topography. A particular application of interest is the spread of a fixed mass – a block – of material under its own weight. The rheology of the fluid is described by the Bingham model which includes the effect of yield stress, i.e. a threshold stress which must be exceeded before flow can occur. Both the plastic viscosity and the yield stress are modelled with temperature-dependent parameters. The flow is described by a reduced model with a thin-film equation for the height of the block and a depth-averaged energy conservation equation for the heat transfer. Results show that for large values of the yield stress, only the outer fraction of the fluid spreads outward, the inner fraction remaining unyielded, hence the block only partially slumps. We present an analysis which predicts the threshold value of the yield stress for which partial slump occurs.