bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wound healing effect of Carum carvi L. on the incised skin wound in male rats: Histopathology, total protein and biomechanical evaluations
Authors: Salari Rafsanjani Mojtaba , Tabatabaei Naeini Aboutorab , Meimandi‐Parizi Abdolhamid , Nowzari Fariborz , Mujtaba Wani Muhammad , Iraji Aida 
Published: October 2022

AbstractBackgroundThe skin has several functions, one of the most important of which is to protect the internal organs from external damage and the entry of germs. Since skin and wound healing is one of the mostly concerned issues worldwide, the development of wound healing remedies is one of the main fields in modern medical research.ObjectivesTo evaluate the effect of the hydroalcoholic extract of Cumin carvi L. seed as one of the traditional medicinal plants used for wound healing through an in vivo model.MethodsWide circular skin wounds (2 × 2 cm) were created on the dorsal area of 50 Sprague–Dawley male rats ​​following ethical principles. The animals were divided into five groups including no treatment, base gel, tetracycline treatment, 10% v/v hydroalcoholic extract of Cumin carvi L. seed treatment and 20% v/v hydroalcoholic extract of Cumin carvi L. seed treatment group. Treatment was performed within 20 days. On days 1, 3, 5, 7 and 10, photographs were taken, and the percentage of wound healing was calculated. Also, on the 10th day, the skin area was sampled for histopathology and on the 20th day, the skin was sampled for biomechanical and total protein assessments.ResultsThe results of wound healing percentage showed that from day 3 onwards, there was a significant improvement between the group treated with 20% v/v hydroalcoholic extract of Cumin carvi L. and the negative control and basal gel groups (p < 0.05). According to histopathological and total protein content evaluations, the amount of collagen production and inflammation score in the Cumin carvi‐treated groups confirmed the healing process compared to other groups.ConclusionsAccording to the results of this project, 20% v/v ethanolic extract of Cumin carvi L. has potential therapeutic effects in the treatment of skin wounds.
Wounds are one of the health concerns, and the economic burden of wound care and healing has continued to increase over the past years.
The healing effects of C. carvi. ethanolic extract on the wound were investigated.
The histopathological and macroscopical evaluations, as well as total protein content, were measured to investigate its wound healing properties.
Single or repeated immunization against GnRH fails to completely abolish spermatogenesis in dwarf bucks (Capra hircus)
Authors: Giriboni Julia , Martínez‐Nevado Eva , García Juncal , Velázquez Rosario , Toledano‐Díaz Adolfo , Ungerfeld Rodolfo , Santiago‐Moreno Julián 
Published: October 2022

AbstractIn both captive wildlife and production animals is important to develop strategies for population control. Immunization against GnRH is an easy and inexpensive immunocastration method that reduces the concentration of testosterone and decreases sperm quality. However, its effectiveness depends on the species and repetition of the treatment. This study aimed to compare the effectiveness of a single treatment (initial immunization plus a booster with Improvac) vs repeated treatment (six doses of Improvac) to inhibit testicular function and maintain the contraceptive status during long periods in bucks. Three Dwarf bucks (Capra hircus) received two doses of Improvac, the first on Week 0, and the booster 4 weeks later (single immunization, group SI) while three Dwarf bucks received one dose of Improvac every 6 months during 3 consecutive years (repeated immunization, group RI). The other three Dwarf bucks remained untreated (control bucks, group CON). Bucks from RI had a greater decrease in scrotal circumference, testosterone concentration, male odor intensity, and sperm quality than SI bucks. However, there were no differences between SI and CON bucks in any of the variables studied. Overall, repeated treatment of Improvac decreased the testicular function of Dwarf bucks, although did not produce complete infertility. However, the repetition of the treatment produced more intensive negative effects, indicating that the strength of the effects of Improvac is rapidly lost in bucks.
Conformal integrals in four dimensions
Authors: Pal Aritra , Ray Koushik 
Published: October 2022

Abstract
We obtain analytic expressions of four-dimensional Euclidean N-point conformal integrals for arbitrary N by solving a Lauricella-like system of differential equations derived earlier. We demonstrate their relation to the GKZ A-hypergeometric systems. The conformal integrals are solutions to these expressed in terms of leg factors and infinite series in the conformal invariant cross ratios.
Soybean genetic resources contributing to sustainable protein production
Authors: Guo Bingfu , Sun Liping , Jiang Siqi , Ren Honglei , Sun Rujian , Wei Zhongyan , Hong Huilong , Luan Xiaoyan , Wang Jun , Wang Xiaobo , Xu Donghe , Li Wenbin , Guo Changhong , Qiu Li-Juan 
Published: October 2022

AbstractKey messageGenetic resources contributes to the sustainable protein production in soybean.AbstractSoybean is an important crop for food, oil, and forage and is the main source of edible vegetable oil and vegetable protein. It plays an important role in maintaining balanced dietary nutrients for human health. The soybean protein content is a quantitative trait mainly controlled by gene additive effects and is usually negatively correlated with agronomic traits such as the oil content and yield. The selection of soybean varieties with high protein content and high yield to secure sustainable protein production is one of the difficulties in soybean breeding. The abundant genetic variation of soybean germplasm resources is the basis for overcoming the obstacles in breeding for soybean varieties with high yield and high protein content. Soybean has been cultivated for more than 5000 years and has spread from China to other parts of the world. The rich genetic resources play an important role in promoting the sustainable production of soybean protein worldwide. In this paper, the origin and spread of soybean and the current status of soybean production are reviewed; the genetic characteristics of soybean protein and the distribution of resources are expounded based on phenotypes; the discovery of soybean seed protein-related genes as well as transcriptomic, metabolomic, and proteomic studies in soybean are elaborated; the creation and utilization of high-protein germplasm resources are introduced; and the prospect of high-protein soybean breeding is described.
Maternal amoxicillin affects piglets colon microbiota: microbial ecology and metabolomics in a gut model
Authors: Nissen Lorenzo , Aniballi Camilla , Casciano Flavia , Elmi Alberto , Ventrella Domenico , Zannoni Augusta , Gianotti Andrea , Bacci Maria Laura 
Published: October 2022

Abstract
The first weeks of life represent a crucial stage for microbial colonization of the piglets’ gastrointestinal tract. Newborns’ microbiota is unstable and easily subject to changes under stimuli or insults. Nonetheless, the administration of antibiotics to the sow is still considered as common practice in intensive farming for pathological conditions in the postpartum. Therefore, transfer of antibiotic residues through milk may occurs, affecting the piglets’ colon microbiota. In this study, we aimed to extend the knowledge on antibiotic transfer through milk, employing an in vitro dedicated piglet colon model (MICODE—Multi Unit In vitro Colon Model). The authors’ focus was set on the shifts of the piglets’ microbiota composition microbiomics (16S r-DNA MiSeq and qPCR—quantitative polymerase chain reaction) and on the production of microbial metabolites (SPME GC/MS—solid phase micro-extraction gas chromatography/mass spectrometry) in response to milk with different concentrations of amoxicillin. The results showed an effective influence of amoxicillin in piglets’ microbiota and metabolites production; however, without altering the overall biodiversity. The scenario is that of a limitation of pathogens and opportunistic taxa, e.g., Staphylococcaceae and Enterobacteriaceae, but also a limitation of commensal dominant Lactobacillaceae, a reduction in commensal Ruminococcaceae and a depletion in beneficial Bifidobactericeae. Lastly, an incremental growth of resistant species, such as Enterococcaceae or Clostridiaceae, was observed. To the authors’ knowledge, this study is the first evaluating the impact of antibiotic residues towards the piglets’ colon microbiota in an in vitro model, opening the way to include such approach in a pipeline of experiments where a reduced number of animals for testing is employed.

Key points
• Piglet colon model to study antibiotic transfer through milk.
• MICODE resulted a robust and versatile in vitro gut model.
• Towards the “3Rs” Principles to replace, reduce and refine the use of animals used for scientific purposes (Directive 2010/63/UE).

Graphical abstract
Negative Pressure Wound Therapy for the Prevention of Surgical Site Infections Using Fascia Closure After EVAR—A Randomized Trial
Authors: Svensson‐Björk Robert , Hasselmann Julien , Asciutto Giuseppe , Zarrouk Moncef , Björk Jonas , Bilos Linda , Pirouzram Artai , Acosta Stefan 
Published: October 2022

AbstractBackgroundSurgical site infections (SSI) in the groin after vascular surgery are common. The aim of the study was to evaluate the effect of negative pressure wound therapy (NPWT) on SSI incidence when applied on closed inguinal incisions after endovascular aneurysm repair (EVAR).MethodsA multicenter randomized controlled trial (RCT). Between November 2013 and December 2020, 377 incisions (336 bilateral and 41 unilateral) from elective EVAR procedures with the primary intent of fascia closure were randomized and included, receiving either NPWT or a standard dressing. In bilateral incisions, each incision randomly received the opposite dressing of the other side, thereby becoming each other's control. The primary endpoint was SSI incidence at 90 days postoperatively, analyzed on an intention‐to‐treat basis. Uni and bilaterally operated incisions were analyzed separately, and their respective p‐values combined using Fisher's method for combining P‐values. Study protocol (NCT01913132).ResultsThe SSI incidence at 90 days postoperatively in bilateral incisions was 1.8% (n = 3/168) in the NPWT and 4.8% (n = 8/168) in the standard dressing group, and in unilateral incisions 13.3% (n = 2/15) and 11.5% (n = 3/26), respectively (combined p = 0.49). In all SSIs, bacteria were isolated from incisional wound cultures. No additional SSIs were diagnosed between 90 days and 1 year follow‐up.ConclusionsNo evidence of difference in SSI incidence was seen in these low‐risk inguinal incisions when comparing NPWT with standard dressings after EVAR with the primary intent of fascia closure.Clinical Trials: NCT01913132.
Optical, electrical and chemical properties of PEO:I2 complex composite films
Authors: Telfah Ahmad , Al-Bataineh Qais M. , Tolstik Elen , Ahmad Ahmad A. , Alsaad Ahmad M. , Ababneh Riad , Tavares Carlos J. , Hergenröder Roland 
Published: October 2022

AbstractSynthesized PEO:I2 complex composite films with different I2 concentrations were deposited onto fused silica substrates using a dip-coating method. Incorporation of PEO films with I2 increases the electrical conductivity of the composite, reaching a maximum of 46 mS/cm for 7 wt% I2. The optical and optoelectronic properties of the complex composite films were studied using the transmittance and reflectance spectra in the UV-Vis region. The transmittance of PEO decreases with increasing I2 content. From this study, the optical bandgap energy decreases from 4.42 to 3.28 eV as I2 content increases from 0 to 7 wt%. In addition, the refractive index for PEO films are in the range of 1.66 and 2.00.1H NMR spectra of pure PEO film shows two major peaks at 3.224 ppm and 1.038 ppm, with different widths assigned to the mobile polymer chains in the amorphous phase, whereas the broad component is assigned to the more rigid molecules in the crystalline phase, respectively. By adding I2 to the PEO, both peaks (amorphous and crystal) are shifted to lower NMR frequencies indicating that I2 is acting as a Lewis acid, and PEO is acting as Lewis base. Hence, molecular iodine reacts favorably with PEO molecules through a charge transfer mechanism, and the formation of triiodide ($${\mathrm{I}}_{3}^{-}$$

I

3

-

), the iodite ($${\mathrm{IO}}_{2}^{-}$$

IO

2

-

) anion, $${\mathrm{I}}_{2} \cdot \cdot \cdot \mathrm{ PEO}$$

I
2

·
·
·
PEO

and $${\mathrm{I}}_{2}^{+} \cdot \cdot \cdot \mathrm{ PEO}$$

I

2

+

·
·
·
PEO

complexes. PEO:I2 complex composite films are expected to be suitable for optical, electrical, and optoelectronic applications.
The preoperative Hounsfield unit value at the position of the future screw insertion is a better predictor of screw loosening than other methods
Authors: Li Jingchi , Zhang Zhuang , Xie Tianhang , Song Zhetao , Song Yueming , Zeng Jiancheng 
Published: October 2022

Abstract
Objective
Screw loosening is a widely reported issue after spinal screw fixation and triggers several complications after lumbar interbody fusion. Osteoporosis is an essential risk factor for screw loosening. Hounsfield units (HU) value is a credible indicator during bone mineral density (BMD) evaluation. As compared with the general evaluation of BMD, we hypothesized that specific measurements of HU at the precise location of the future screw insertion may be a better predictor of screw loosening.

Methods
Clinical data of 56 patients treated by oblique lumbar interbody fusion (OLIF) of the L4-L5 segments with an anterior lateral single rod (ALSR) screw fixation were reviewed in this study. Vertebral bodies with ≥ 1 mm width radiolucent zones around the screw were defined as screw loosening. HU in the insertional screw positions, the central transverse plane, and the average values of three and four planes were measured. Regression analyses identified independent risk factors for screw loosening separately. The area under the receiver operating characteristic curve (AUC) was computed to evaluate predictive performance.

Results
The local HU values were significantly lower in the loosening group, regardless of the selected measuring methods. The AUC of screw loosening prediction was higher in the insertional screw positions’ HU than other frequently used methods.

Conclusions
The HU value measured in the insertional screw position is a better predictor of ALSR screw loosening than other methods. The risk of screw loosening should be reduced by optimizing the trajectory of the screw based on the measurement of HU in preoperative CT.

Key Points
• Osteoporosis is an essential risk factor for screw loosening, and Hounsfield units (HU) are a credible predictor during bone mineral density (BMD) evaluation.
• The HU value measured in the insertional screw position is a better predictor of screw loosening than other frequently used HU measurement methods.
• The risk of screw loosening might potentially be reduced by optimizing the trajectory of the screw based on the measurement of HU in preoperative CT.
In-flight measurement of free-stream turbulence in the convective boundary layer
Authors: Greiner Michael , Würz Werner 
Published: October 2022

AbstractNatural laminar flow airfoils have achieved such a level of refinement that further optimisation and subsequent wind tunnel testing need to regard the specific free-stream turbulence to be expected during operation. This requires the characterisation of this turbulence in terms of those properties which are relevant for boundary layer receptivity and subsequent transition. These parameters of turbulence change with environmental conditions and, in case of aircraft, along the flight profile. This study investigates the free-stream turbulence relevant for the case of sailplane airfoils. In-flight measurements with a constant temperature anemometer x-wire probe were conducted during cross-country flights in Central Europe and provided 22 h of flight data, covering thermalling phases as well as straight flight legs. Longitudinal and transversal velocity fluctuations were recorded well into the dissipation range. The special challenges of operating a constant temperature anemometer probe continuously for several hours are addressed. The permanent unsteadiness of the inflow poses challenges for the evaluation, but also provides a broad database of measured turbulence levels. The quality of the measurements is shown by verifying some of the predictions of Kolmogorov's inertial range theories. Free-stream turbulence in thermalling phases is sufficiently homogeneous to be described accurately, as the dissipation range fluctuates only in a limited range and follows a log-normal distribution. On the straight flight legs, the turbulence depends on the convective activity along the flight path. In general, within the convective part of the atmosphere, turbulence levels are found to be significantly larger than in low-turbulence wind tunnels.Graphical abstract
Implanted haemodynamic telemonitoring devices to guide management of heart failure: a review and meta-analysis of randomised trials
Authors: Iaconelli Antonio , Pellicori Pierpaolo , Caiazzo Elisabetta , Rezig Asma O. M. , Bruzzese Dario , Maffia Pasquale , Cleland John G. F. 
Published: October 2022

Abstract
Background and aims
Congestion is a key driver of morbidity and mortality in heart failure. Implanted haemodynamic monitoring devices might allow early identification and management of congestion. Here, we provide a state-of-the-art review of implanted haemodynamic monitoring devices for patients with heart failure, including a meta-analysis of randomised trials.

Methods and results
We did a systematic search for pre-print and published trials in Medline, Embase, and the Cochrane Central Register of Controlled Trials (CENTRAL) on the 22nd of September 2021. We included randomised trials that compared management with or without information from implanted haemodynamic monitoring devices for patients with heart failure. Outcomes selected were hospitalisation for heart failure and all-cause mortality. Changes in treatment associated with haemodynamic monitoring resulted in only a small reduction in mean pulmonary artery pressure (typically < 1 mmHg as a daily average), which generally remained much greater than 20 mmHg. Haemodynamic monitoring reduced hospitalisations for heart failure (HR 0.75; 95% CI 0.58–0.96; p = 0.03) but not mortality (RR 0.92; 95% CI 0.68–1.26; p = 0.48).

Conclusions
Haemodynamic monitoring for patients with heart failure may reduce the risk of hospitalization for heart failure but this has not yet translated into a reduction in mortality, perhaps because the duration of trials was too short or the reduction in pulmonary artery pressure was not sufficiently large. The efficacy and safety of aiming for larger reductions in pulmonary artery pressure should be explored.

Graphical abstract
Epidemiology of cardiac amyloidosis in Germany: a retrospective analysis from 2009 to 2018
Authors: Ney Svenja , Ihle Peter , Ruhnke Thomas , Günster Christian , Michels Guido , Seuthe Katharina , Hellmich Martin , Pfister Roman 
Published: October 2022

Abstract
Background
Improved imaging modalities contributed to increasing awareness of cardiac amyloidosis. Contemporary data on frequency trends in Germany are lacking.

Methods
In a retrospective study using health claims data of a German statutory health insurance, patients with diagnostic codes of amyloidosis and concomitant heart failure between 2009 and 2018 were identified.

Results
Prevalence increased from 15.5 to 47.6 per 100,000 person-years, and incidence increased from 4.8 to 11.6 per 100,000 person-years, with a continuous steepening in the slope of incidence trend. In patients with amyloidosis and heart failure age and proportion of men significantly increased, whereas the frequency of myeloma and nephrotic syndrome significantly decreased over time. Median (IQR) survival time after first diagnosis was 2.5 years (0.5–6 years), with a 9% (95% CI 2–15%, p = 0.008) reduced risk of death in the second compared to the first 5 years of observation. In the 2 years prior and 1 year after diagnosis, mean total health care costs were 6568 €, 11,872 € and 21,955 € per person and year.

Conclusion
The rise in cardiac amyloidosis has continuously accelerated in the last decade. Considering the adverse outcome and high health care burden, further effort should be put on early detection of the disease to implement available treatment.
Retrosplenial and subicular inputs converge on superficially projecting layer V neurons of medial entorhinal cortex
Authors: Simonsen Øyvind Wilsgård , Czajkowski Rafał , Witter Menno P. 
Published: October 2022

AbstractThe medial entorhinal cortex (MEC) plays a pivotal role in spatial processing together with hippocampal formation. The retrosplenial cortex (RSC) is also implicated in this process, and it is thus relevant to understand how these structures interact. This requires precise knowledge of their connectivity. Projections from neurons in RSC synapse onto principal neurons in layer V of MEC and some of these neurons send axons into superficial layers of MEC. Layer V of MEC is also the main target for hippocampal efferents from the subiculum and CA1 field. The aim of this study was to assess whether the population of cells targeted by RSC projections also receives input from the hippocampal formation and to compare the distribution of synaptic contacts on target dendrites. We labeled the cells in layer V of MEC by injecting a retrograde tracer into superficial layers. At the same time, we labeled RSC and subicular projections with different anterograde tracers. 3D-reconstruction of the labeled cells and axons revealed likely synaptic contacts between presynaptic boutons of both origins and postsynaptic MEC layer V basal dendrites. Moreover, these contacts overlapped on the same dendritic segments without targeting specific domains. Our results support the notion that MEC layer V neurons that project to the superficial layers receive convergent input from both RSC and subiculum. These data thus suggest that convergent subicular and RSC information contributes to the signal that neurons in superficial layers of EC send to the hippocampal formation.