bluuebunny/crossref_metadata_2025_split huggingface dataset:
Minimizing N-related environmental harm associated with food security in China
Authors: Liu Xuejun , de Vries Wim , Zhang Ying , Liu Lei , Ma Lin , Cui Zhenling , Zhu Qichao , Ying Hao , Fan Mingsheng , Zhang Weifeng , Goulding Keith , Misselbrook Tom , Chadwick David , Zhang Jie , Zhang Fusuo 
Published: October 2022

Abstract
Elevated inputs of nitrogen (N) fertilizer have played a key role in feeding an increasing global population, but also caused many environmental problems due to emissions of reactive N (Nr) to air and water worldwide, especially in China. To better understand the improvements to N management required to reduce impacts in China, we compiled a long-term N budget over the period 1961-2018, quantified historic changes in N inputs, crop uptakes and losses, and compared them with required N inputs to satisfy food security and critical N inputs to protect the environment in view of acceptable losses of ammonia to air and nitrate to water. Nitrogen inputs required to satisfy the food demand of China were 14, 21, 27 and 29 Tg N yr-1 in 1961, 1980, 2000 and 2018, respectively, while the actual N inputs were 4, 25, 39 and 48 Tg N yr-1, respectively, exceeding the required input after 1980. Since 2000, the actual N inputs also exceeded the critical N inputs in view of ammonia emissions to air (35 Tg N yr-1) and nitrate leaching to groundwater (28 Tg N yr-1) that are needed to protect biodiversity and human health. We show that a reduction in N input from 48 to 31 Tg N yr-1, which reconciles food production with environmental safety, can be achieved through the effective recycling of manure, balanced N fertilization and integrated soil-crop system management. Such a 'win-win' would improve air and water quality and protect the environment and human health through improved resource use efficiencies, reduced N fertilizer use and decreased Nr losses.
PCR-like Performance of Rapid Test with Permselective Tunable Nanotrap
Authors: Park Seong Jun , Lee Seungmin , Lee Dongtak , Lee Na Eun , Park Jeong Soo , Hong Ji Hye , Jang Jae Won , Kim Hyunji , Roh Seokbeom , Lee Gyudo , Lee Dongho , Cho Sung-Yeon , Park Chulmin , Lee Dong-Gun , Lee Raeseok , Nho Dukhee , Yoon Dae Sung , Yoo Yong Kyoung , Lee Jeong Hoon 
Published: October 2022

Abstract
Highly sensitive rapid testing for COVID-19 is essential for minimizing virus transmission, especially before recognizing symptoms and in asymptomatic cases. Here, we show bioengineered enrichment tools for LFA with enhanced sensitivity and selectivity (BEETLES2), achieving enrichment of SARS-CoV-2 viruses, nucleocapsid (N) proteins and immunoglobulin G (IgG) with 3 min operation. The limit of detection is improved up to 20-fold. With clinical samples, including 83% of intermediate (35%) and low viral loads (48%), collected from 62 individuals (n = 42 for positive and n = 20 for healthy controls), we observed that the diagnostic sensitivity, specificity, and accuracy reached 88.1%, 100%, and 91.9%, respectively, compared with those of commercial LFA (14.29%, 100%, and 41.94%, respectively). BEETLES2, with permselectivity and tunability, can enrich the SARS-CoV-2 virus, N proteins, and IgG in the nasopharyngeal/oropharyngeal swab, saliva, and blood serum, enabling reliable and sensitive point-of-care testing, facilitating fast early diagnosis.
Antibiotic-impregnated calcium sulfate for the treatment of pediatric hematogenous osteomyelitis
Authors: Tao Rui , Wu Jian-qun , Luo Ji-wei , Hong Liang , Zhou Chun-hao , Cheng Guo-yun , Qin Cheng-he 
Published: October 2022

Abstract

Background Antibiotic-impregnated calcium sulfate has excellent curative efficacy in chronic osteomyelitis. However, its curative efficacy in pediatric hematogenous osteomyelitis has not been sufficiently studied. The purpose of this study was to evaluate the curative effects of antibiotic-impregnated calcium sulfate in the treatment of pediatric hematogenous osteomyelitis. Methods Overall, twenty-one pediatric patients with hematogenous osteomyelitis treated in our hospital were included for assessment between 2013 and 2018. The clinical history, clinical manifestation, infection recurrence rate, sinus leakage, incision leakage, pathological fractures, bone growth and surgical procedures were analyzed. Results The infection recurrence rate was 0% (0/21) at a minimum of 31 months (range 31 to 91 months) of follow-up. Incision leakage postoperatively was found in one pediatric patient. Osteolysis was found in one pediatric patient. Acceleration of bone growth occurred in one pediatric patient. Retardation of bone growth occurred in one pediatric patient. Genu valgus deformity occurred in one pediatric patient. Conclusions Although exist non-infective complications, the curative effect of antibiotic-impregnated calcium sulfate in pediatric hematogenous ostemyelitis was satisfactory.
Growth of an entire function on the basis of relative (p, q)-th order in terms of central index
Authors: Bhan Suraj , Rastogi Anupma 
Published: October 2022

Abstract
In this paper, we study some comparative growth properties of composite entire functions on the basis of relative (p,q)-th order and relative (p,q)-th lower order of entire function with respect to another entire function where p and q are any two positive integers, here we also take relative L order and relative L * order form, to prove some comparative growth properties.
Long-term in situ restoration of different arbors in coal areas reconfigures metabolite and bacterial symbiosis patterns in vertical soils
Authors: WANG Yi-wang , BAI Dong-sheng , ZHANG Yu , LUO Xue-gang 
Published: October 2022

Abstract
Aims
To elucidate the dominant effects of long-term restoration of different woody plants on the reconstitution of multiple environmental and biological factors in vertical soils of coal mining areas.
Methods
Soil physicochemical properties, soil enzyme activities, soil ionophore, microbial community structure, and soil metabolite distribution characteristics and interaction networks were analyzed at different vertical depths after long-term restoration (20 years) of four woody plant species in the coal mine area.
Results
(1) Phytoremediation increased the soil urease, sucrase, and acid phosphatase activities by 4.23–8.82, 4.52–8.29, and 3.28–5.26 times at 10 cm depth. It also reduced the active element content in the soil, and alleviated the mine toxicity stress of S, Fe, As, Pb, and U in the soil. (2) The Richness index and Shannon index of soil microorganisms increased by 4.77–23.81% and 2.93–7.93% after long-term restoration of woody plants, and the bacterial ecological niche was broadened. The microbial community structure shaped by different woody plants was highly similar, but the community similarity decreased with the increase in relative distance at vertical depth. Soil Cu, Fe, Mo, Na, and pH were important influencing factors of community structure for Sphingomonas, Pseudarthrobacter, Nocardioides, and Thiobacillus. (3) Woody plant restoration increased the abundance of soil metabolites (10 cm depth), and induced metabolites such as Organooxygen compounds, Carboxylic acids and derivatives, and Fatty Acyls as the main microbial regulators.
Conclusions
Woody plants have different patterns of environmental and biometabolic factor configurations for different depths of soil during mine restoration.
Impact of Farmers-Herders Conflict on Socio-Economic Development of Oke-Ogun area of Oyo State
Authors: SALISU Temitope Quadri 
Published: October 2022

AbstractThis study investigated the impact of farmers-herders conflict on socio economic development of Oke-Ogun area of Oyo State. The study used survey method to gather the relevant data by structuring three hundred and fifty (350) questionnaires in order to gather some necessary and relevant information from respondents. In other to test the impact on farmers-herders conflict on the residence of the Oke-Ogun area of Oyo State oral interview was also conducted. The qualitative data collected were analysed using content analysis verbation quotations and thematic analysis. The findings of the study research question one revealed there has been different causes of farmers-herders conflict from various dimension. Using response from the highest number of the respondents, it was found that land dispute, destruction of crops and disregards for local traditional authorities are the major causes of farmers-herders conflict in Oke-Ogun area of Oyo State. Moreso, the study found that lots of damages were recorded as a result of the conflict between both parties which includes loss of lives, properties, homes etc. This study recommends that Government (policy makers) should set up of administrative machinery for peace and conflict resolution as well as the encouragement of constant dialogue between farmers and herders
Thoracic anterior spinal cord herniation: treatment and prognosis outcome of 15 patients and review of the literature
Authors: Jiang Qingyu , Gao Gan , Tao Benzhang , Gao Haihao , Wang Hui , Wang Peixin , Sun Mengchun , Shang Aijia 
Published: October 2022

Abstract
Background: This study aimed to investigate the treatment and curative effects of thoracic anterior spinal cord herniation.
Methods: Clinical data of 15 patients with thoracic anterior spinal cord herniation were retrospectively analysed. All patients were diagnosed with a complete pre-operative examination and scheduled for surgical treatment. In addition, regular follow-up was performed after the surgery, and the operation’s efficacy was evaluated according to clinical symptoms, imaging findings, and improvement in neurological function.
Results: All 15 patients underwent spinal cord release with an anterior dural patch. Notably, no severe post-operative surgical complications were observed. All patients were followed up for 3–108 months, with an average duration of approximately 52.9 months. Post-operative pain symptoms were effectively controlled, neurological dysfunction and related symptoms improved to varying degrees, and anterior spinal cord herniation did not recur. The modified Japanese Orthopaedic Association score at the last follow-up was significantly higher than the pre-operative score.
Conclusions: Clinicians should avoid misdiagnosing patients with thoracic anterior spinal cord herniation with intervertebral disc herniation, arachnoid cysts, and other related diseases, and patients should undergo surgical treatment as early as possible. In addition, surgical treatment can protect the neurological function of patients and effectively prevent the aggravation of clinical symptoms.
Community participation for COVID-19 pandemic preparedness and response: A systematic review
Authors: Abayneh Aschalew , Sasie Sileshi Demelash , Aragaw Fantu Mamo , Azage Muluken , Mengesha Selamawit , Spigt Mark 
Published: October 2022

Abstract
Introduction:
Community engagement is an integral part of any preparedness and response to an emergency. Therefore, community center approaches must be developed during public health emergency preparedness and response. The main objectives of this systematic review were to identify existing evidence of community engagement and participation in relation to COVID-19 pandemic and to better prepare for potential future outbreaks in the local context.
Methods
Databases searched included PubMed, CINAHL, and CABI. Grey literature sources included greylit.org, opengrey.eu, worldcat.org, and evidenceaid.org. Individual international organization, national government and non-governmental organization (NGO) websites were also searched, both directly and using the Google site search.
Results
The results of this review indicated that community engagement is very essential for the COVID-19 pandemic preparedness and response. By involving communities in COVID-19 preparation and response, the health sector can avoid the emergence of cases that would worsen the pandemic. Engaging communities should play a central role in COVID-19 pandemic preparedness responses by building trust and allowing the community to enter in the system starting from the designing and planning of emergency preparedness and response.
Conclusion
Community engagement is very crucial for effective community response and preparedness. Concerned bodies should immediately set up specific community engagement taskforces to ensure for effective pandemic response and preparedness. It is very crucial build the capacities of local stakeholders in supporting communities to respond to different health challenges and threats. The results indicate that new approach for large-scale community engagement in future epidemics and other health emergencies.
Phytochemical and biological evaluation of stem bark of Holarrhena pubescens against oxidative and inflammatory disorders
Authors: Dhiman Sakshi , Nadda Rohit Kumar , Thakur Ruhi , Goyal Rohit , Bhardwaj Prerna 
Published: October 2022

Abstract
Background
Holarrhena has been traditionally used to treat inflammatory disorders and oxidative stress. The purpose of our study was to examine the biological potential of phytoconstituents found in the stem bark of H. pubescens using antioxidant and anti-inflammatory in-vitro methods.
Method
Dried stem bark of this plant was firstly extracted with n-hexane for defatting and then extracted with hydro-alcohol (90% ethanol). The hydro-alcoholic extract was further fractionated by different solvents (chloroform, ethyl acetate and butanol) and the antioxidant activity was assessed using reducing power assay, free radical scavenging methods for 1,1-diphenyl-2-picrylhydrazyl (DPPH), and hydrogen peroxide radicals. Anti-inflammatory activities were carried out by using albumin denaturation and nitric oxide (NO) assays.
Results
The qualitative phytochemical analysis of n-hexane and hydro-alcoholic extract revealed the presence of alkaloids, saponins, flavonoids, phenols, carbohydrates, proteins and tannins. In quantitative phytochemical analysis of hydro-alcoholic extract showed a higher percentage of phytochemicals than n-hexane extract. Hydro-alcoholic extract and fractions showed significant antioxidant activities against DPPH, H2O2, and reducing power assay. Similarly, in anti-inflammatory studies, butanol fraction showed higher protection against albumin denaturation which was 55.81 ± 2.05 whereas in NO assay ethyl acetate fraction was 30.39 ± 1.37.
Conclusion
Our research concluded that the hydro-alcoholic extract has highly phytochemical properties than n-hexane extract whereas ethyl acetate and butanol fraction showed superior anti-oxidant and anti-inflammatory properties as compared to other fractions. This research supports the plant's traditional use as a medicine.
Identification of lipid droplet-associated genes in breast cancer patients
Authors: Dogan Senol , Leopold Jenny , Hoffmann Daniel T. , Kubitschke Hans , Blauth Eliane , Ficorella Carlotta , Zschau Amelie , Schiller Jürgen , Käs Josef A. 
Published: October 2022

Abstract

Background: Abnormal lipid metabolism plays an essential role in breast cancer progression and metastasis. Lipid droplets (LD) have multifunctional tasks as they store and transfer lipids and act as molecular messengers. In particular, they are known to be involved in reprogramming tumor cells, invasion, and migration of breast cancer cells. In this study, we aimed to identify lipid droplet-associated genes as prognostic markers in breast cancer.
Methods: Established lipid droplet-associated proteins were used to create the research gene lists. Bioinformatics analysis on the GEPIA platform was carried out for the list of the genes to identify differential expression in breast cancer versus healthy breast tissues. Differentially expressed genes were analyzed regarding significant changes during the metastatic transition and detected genes which play a role in breast cancer patients. Changes in lipid composition were monitored by mass spectrometry. In more detail, immunohistochemistry and cell culture studies were performed to understand the LD-related proteins and lipids in the cell lines.
Results: 143 genes were identified as lipid droplet-associated factors by literature research. Bioinformatics analysis of 1085 breast cancer samples and 291 normal breast tissue samples identified 48 differentially expressed genes in breast cancer with 3 over-expressed genes (SQLE, FADS2, MUCI) and 45 under-expressed genes. Among 48 differentially expressed genes, only one over-expressed gene
(SQLE) and 5 under-expressed genes (FABP7, SAA4, CHKB, RBP4, PLA2G4A) were significantly associated with the overall survival of breast cancer patients. While 26 of these genes were also found in the metastatic transition, the expression of only 13 of them changed in cancer. SELP, FABP4, and PLIN1 were detected as the highest F-value in the transitions of metastatic stages. OSBPL2, CPA4, DGAT1, and FADS6 were effective genes in both overall survival and metastatic transition. Among all these genes, only FABP7 showed a statistically significant rank in all criteria as a prognostic factor. Changes in the lipid compositions, size and radii of lipid droplets were also be monitored and combined with bioinformatics analysis.
Conclusions: Through bioinformatics analysis, 29 prognostically relevant differentially expressed genes were identified. 26 genes play a role during the metastatic transition highlighting the role of lipid droplet-associated factors in breast cancer.
Comprehensive analysis of PCM container construction effects PV panels thermal management
Authors: Metwally Hesham , Mahmoud N. A. , Aboelsoud W. , Ezzat M. 
Published: October 2022

Abstract
Current research aims to identify the finest phase change material container construction and tries to close the design gap for optimum photovoltaic panel thermal management. The phase change material is used as heat sink of photovoltaic panel and heat source for thermoelectric generator. The latent heat of phase change material maximizes the power generation from thermoelectric generator. The results show that the efficiency of the photovoltaic panel was enhanced by 3% and steady for ten hours. The photovoltaic panel electrical output power was enhanced by 25% under different weather conditions. The hybrid cooling system with capsules phase change material cavity shows a significant enhancement and stability in thermal system management, photovoltaic efficiency, and system output power.
Deciphering Bacterial and Resistome Compositions of Sewage Samples from a Major Wastewater Treatment Plant in Northern Mexico: A Metagenomics Approach
Authors: Oyedara Omotayo Opemipo , Ruiz-Amaro Carlos Jesús , Heredia Norma , García Santos 
Published: October 2022

Abstract
Wastewater treatment plants (WWTPs) are hotspots for pathogens, antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and horizontal gene transfer (HGT) because of the inflow of nutrient-rich organic wastewater they receive from different sources including, household, hospitals, and industries. Bacterial communities and ARGs of raw (influent) and treated (effluent) sewage samples collected in March 2021 and June 2022 from a WWTP in Northern Mexico were studied using nanopore sequencing technology. Proteobacteria (52.56–61.50%), Bacteroidetes (8.70-15.58%), Actinobacteria (7.45–12.86%), and Firmicutes (2.78–21.27%) were the major phyla detected in all the sewage samples. The genus Arcobacter (15.36–29.11%) dominated all the sewage samples, except in the effluent collected in 2022, where more abundance of the genus Shewanella (7.51%) and Aeromonas (6.12%) was observed. Multidrug (antibiotics efflux) resistance genes were the major ARGs (28.30-36.61%) detected in the samples by the NanoARG platform. ARGs classes common to the samples include glycopeptide (vanR and vanRI), bacitracin (bacA and bcrA), macrolide (LlmA and macB), fluoroquinolone (patA), peptide (ugd), tetracycline (otrC), and phenicol (clbB). Beta-lactam, tetracycline, MLS, aminoglycosides, and sulphonamide have more ARGs associated with plasmids. The members of the clinically relevant ESKAPE group (Enterococcus faecium, Klebsiella pneumoniae, and Acinetobacter baumannii), E. coli, Eubacterium rectale, and Acinetobacter johnsonii with mobile genetic elements carrying ARGs were among the potential human pathogens detected in effluent samples. The release of effluents containing these bacteria or their genomes into the natural environment could have public health implications and aid the spread of ARGs. Better policies and enhanced wastewater treatment strategies are necessary to reduce or eliminate these risks.
Automated Image Analysis Pipeline Development to Monitor Disease Progression in Muscular Dystrophy Using CellProfiler
Authors: Brown Alexandra , Alshudukhi Abdullah A. , Jama Abdulrahman , Kamau John Karanja , Ren Hongmei 
Published: October 2022

Abstract
Muscular dystrophies are inherited disorders that are characterized by progressive muscle degeneration. These disorders are caused by mutation in the genes encoding structural elements within the muscle, which leads to increased vulnerability to mechanical stress and sarcolemma damage. Although myofibers have the capacity to regenerate, the newly formed myofibers still harbor the genetic mutation, which induces continuous cycles of muscle fiber death and regeneration. This repeated cycling is accompanied by an inflammatory response which eventually provokes excessive fibrotic deposition. The histopathological changes in skeletal muscle tissue are central to the disease pathogenesis. Analysis of muscle histopathology is the gold standard for monitoring muscle health and disease progression. However, manual, or semi-manual quantification methods are not only immensely tedious but can be subjective. Here, we present four image analysis pipelines built in CellProfiler which enable users without a background in computer vision or programming to quantitatively analyze biological images. These image analysis pipelines are designed to quantify skeletal muscle histopathological staining for membrane damage, the abundance and size distribution of regenerating muscle fibers, inflammation via quantification of CD68 + M1 macrophages, and collagen deposition. Additionally, we discuss methods to address common errors associated with the quantification of microscopy images. These automated tools can not only improve workflow efficiency but can provide a better understanding of the histopathological progression of muscular dystrophy.
A selective hybrid system for State-of-Charge forecasting of Lithium-ion batteries
Authors: Medina Marie Chantelle Cruz , de Oliveira João Fausto Lorenzato 
Published: October 2022

Abstract
As research in Lithium-ion batteries field has extended, the need for better management systems also increases. An important part of them is the proper estimation of battery status over time with indirect metrics such as State-of-Charge (SoC). In the machine learning environment, different simple techniques have been tested showing good performance and being surpassed by hybrid systems.In this study, a static selection model is proposed to choose the best non-linear predictor to work with and ARIMA model and combination function for a specific database considering how the perform in a validation set. This architecture allows to consider linear and non-linear components of the time series using residual forecasting and a selection step to reduce the chances of choosing the wrong combination in each specific database.SoC values for five different databases where forecasted, allowing to compare the results of six models relevant in literature to the proposed one.The results showed a superior performance for the proposed model in four out of the five databases, with gains of 5.27%, 13.51%, 56.67%, and 38.71%. There was only one database where the proposed model scored in second place. To determine whether the obtained results were statistically significant enough to make a conclusion, a Nemenyi test was conducted using MSE and MAE values to rank the performance of all models in all databases. The critical distance and rank achieved by the proposed model allowed to conclude that this, in fact, delivered the best performance amongst all models tested.