bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of clinical outcomes between new T-shaped and variable-angle volar locking plates for the treatment of distal radius fractures.
Authors: Zhou Dong , Wu Shuting , Wang Lei , Su Yu , Liu Sihui , Guo Weizhong 
Published: November 2022

Abstract

Background:New T-shaped volar locking plates (VLPs) and variable-angle volar locking plates (VLPs) are utilized for the internal fixation of distal radius fractures (DRFs). However, studies regarding the difference in clinical outcomes between these two plates are limited. The purpose of the present study was to compare postoperative outcomes between these two plate types for DRFs.
Materials and Methods: A total of 45 consecutive patients (23 patients with new T-shaped VLPs and 22 patients with variable-angle VLPs) who underwent surgical treatment for DRFs were reviewed for this retrospective study. The range of motion (ROM), grip strength, visual analogue scale (VAS) pain score, X-ray findings, complication rate, fracture healing time and patient satisfaction with current activity were compared.
Results: The analysis of wrist ROM revealed that there were no statistically significant differences (p > 0.05) in extension, flexion, ulnar deviation, radial deviation, supination, pronation or grip strength between the two groups. There were no statistically significant differences (p > 0.05) between the two groups with regard to alignment and fracture healing on X-ray. The final VAS pain score and patient satisfaction with current activity differed between the two groups. The complication rate in the variable-angle VLP group was significantly different from the 0% rate observed in the new T-shaped VLP group (P < 0.05).
Conclusions: Although use of the new T-shaped VLP for the treatment of DRFs did not show superiority in functional outcomes or radiological findings over variable-angle VLPs, the present findings showed that internal fixation of unstable DRFs using the new T-shaped VLP yielded better clinical results with fewer complications.
Time-delay identification from chaotic time series via statistical complexity measures based on ordinal pattern transition networks
Authors: He Xin , Sun zhongKui 
Published: November 2022

Abstract
In this paper, a methodology based on the nonlinear time series analysis complex network theory to identify time-delay parameters from the chaotic time series is proposed for the first time, to accurately and rapidly reveal the intrinsic time-delay characteristics for the underlying dynamics. More exactly, we discover that time-delay parameters can be identified from chaotic time series by using two statistical complexity measures (SCMs) respectively, which are defined by two normalized ways of the ordinal pattern transition matrix of ordinal pattern transition networks (OPTNs). The prime advantage of the proposed method is straightforward to apply and well robustness to dynamical noises and observational noises. Some other merits were discovered including: A comparative research of the new technique with the permutation-information-theory approach shows that the identifying performance is improved to two orders of magnitude at least for the dynamical Gaussian white noise. And the new method also identifies two time-delay parameters for the condition of relatively short time series, but the traditional delayed mutual information technology cannot.
Low level incorporation of Lactobacillus Sporogenes in feed of Tinfoil Barb (Barbonymus Schwanenfeldii (Bleeker, 1854)) Fingerlings Positively Improves Growth, Gut Microflora and Intestinal Morphology
Authors: Vutukuri Manjusha , Ghode Gajanan , Pawase Anil S , Kouser Shaik , Tibile R. M. 
Published: November 2022

Abstract
Use of probiotics in freshwater fishes is debated for its benefits to the host. Tinfoil barb, Barbonymus schwanenfeldii is a tropical fish having ornamental as well as food value. This study was conducted to evaluate effect of Lactobacillus sporogenes on Tinfoil barb. Diets containing 0%(T0), 0.5%(T1), 1%(T2), 1.5%(T3), and 2% (T4) of probiotic L. sporogenes were prepared and fed to fishes at 3% body weight for 80 days. Control diet was fed to fishes of all treatments for 20 more days. Growth parameters, gut microflora and intestinal histology were analysed. The growth performance was found to be significantly better (P < 0.05) in fishes fed with 1% of probiotic but Survival remained unaffected due to the concentration of probiotic. Total Plate Count of aerobes seemed to be affected by concentration and duration of probiotic treatment. However, anaerobic TPC did not show much variation. Histological analysis revealed that in lower level of incorporation (T2-1%) fish have intact mucosal layer with increased absorptive enterocytes and absorptive area of intestinal villi, well organized lamina propria with different types of cells compared to other treatments. The results indicated that addition of L. sporogenes at 1% level of inclusion was found to be effective for better growth, survival, gut flora as well as intestinal morphology in B. schwanenfeldii.
Significance of metagenomic next-generation sequencing in the diagnosis and prognosis for spontaneous peritonitis in patients with cirrhosis
Authors: Xiao Peng , Xu Hongqin , Zhang Feiyu , Liu Tao , Gao Yanhang 
Published: November 2022

Abstract
Background
The most common infectious complication in patients with cirrhosis is spontaneous bacterial peritonitis (SBP), which is directly linked to the prognosis of patients with decompensated cirrhosis. The standard diagnostic criteria for SBP are insensitive, and early detection of SBP is difficult. Despite the evident benefits of metagenomic next-generation sequencing (mNGS) in the pathogens diagnosis of many infectious diseases, few data on the diagnosis and prognosis of SBP in patients with cirrhosis.
Methods
Sixty patients with cirrhotic ascites were enrolled from July 11, 2020, to December 15, 2020, who underwent ultrasound-guided abdominal puncture at Jilin University's First Hospital. Ascites samples of these patients were collected for mNGS detection and conventional pathogen culture. Patients were prospectively followed up on for 28 days and 90 days after enrolment. Finally, data on liver transplantation as well as other adverse events, death, and reasons for death were gathered prospectively in all patients 28 and 90 days after enrollment.
Results
Ascites mNGS technology can increase the positive rate of pathogens in cirrhotic patients with ascites when compared to ascites culture. The mNGS detection results are more detailed than ascites culture results, and they can help clinicians use antibiotics sooner. The positive rate of ascites PMN combined with ascites mNGS detection in the diagnosis of SBP in cirrhotic patients was significantly higher than that of ascites PMN combined with ascites culture. In cirrhotic patients with ascites, the mNGS assay was more accurate than the ascites culture in predicting adverse outcomes during the 28-dayand 90-day follow-up periods.
Conclusions
The ascites mNGS assay has greater diagnostic and prognostic value than bacterial culture for cirrhotic patients. In clinical practice, ascites mNGS detection should be conducted as soon as possible in patients with suspected SBP so that clinicians can identify and treat SBP earlier and improve prognosis.
Aging-related genes related to the prognosis and the immune microenvironment of acute myeloid leukemia
Authors: Gang dongxu , Jiang yinyan , Wang xiaofang , Zhou jifan , Zhang xiaoyuan , He xiaoyu , Dong rujiao , Huang ziyang , Jiang songfu 
Published: November 2022

Abstract
Background: Acute myeloid leukemia (AML), one of the most common malignancies of the hematologic system, has progressively increased in incidence. Aging is present in both normal tissues and the tumor microenvironment. However, the relationship between senescence and AML prognosis is still not elucidated.
Methods: In this study, RNA sequencing data of AML were obtained from TCGA, and prognostic prediction models were established by LASSO-Cox analysis. Differences in immune infiltration between the different risk groups were calculated using the CIBERSORT and ESTIMATE scoring methods. The KEGG and GO gene enrichment and GSEA enrichment were also used to enrich for differential pathways between the two groups. Subsequently, this study collected bone marrow samples from patients and healthy individuals to verify the differential expression of uncoupling protein 2 (UCP2) in different populations. Genipin, a UCP2 protein inhibitor, was also used to examine its effects on proliferation, cell cycle, and apoptosis in AML cell lines in vitro.
Results: It showed that Aging-related genes (ARGs) expression was correlated with prognosis. And there was a significant difference in the abundance of immune microenvironment cells between the two groups of patients at high risk and low risk. Subsequently, UCP2 expression was found to be elevated in AML patients. Genipin inhibits UCP2 protein and suppresses the proliferation of AML cell lines in vitro.
Conclusion: ARGs can be used as a predictor of prognosis in AML patients. Moreover, suppressing UCP2 can reduce the proliferation of AML cell lines, alter their cell cycle and promote apoptosis in vitro.
Rilmenidine protects against joint damage in MIA-induced model of osteoarthritis in rats
Authors: Kukula Osman , Çiçekli Mustafa Nusret , Şarklıoğlu Selenay Sevinç , Kırmızıkan Seda , Günaydın Caner 
Published: November 2022

Abstract
Rilmenidine protects against joint damage in MIA-induced model of osteoarthritis in rats
Purpose: Osteoarthritis is a common problem, and its incidence significantly increases with age. Patients suffer from excruciating pain while moving, and currently, major treatment options consist of surgery. Rilmenidine is a potent antihypertensive agent with a high affinity for imidazoline and alpha2 adrenergic receptors. Based on the knowledge that these receptors are also related to bone turnover and pain, we aimed to reveal the effect of rilmenidine on the osteoarthritis model in rats.
Methods: Monosodium iodoacetate(MIA) was used to induce osteoarthritis. Animals were treated with rilmenidine(0.5, 2 mg/kg) for 14 days. Hot plate test was performed to assess pain response before and end of the drug treatments, in addition to the walking track analysis. Twenty-four hours after the last drug treatment, serum levels of receptor activator of nuclear factor kappa-Β ligand(RANKL) and osteoprotegerin(OPG) were measured. Hematoxylin&eosin and safranin-O staining were used to evaluate MIA and rilmenidine induced changes in the hindlimb joints.
Results: Our results demonstrated that rilmenidine(2 mg/kg) prevented MIA-induced thermal hyperalgesia with improved walking behavior in the walking track test. Additionally, rilmenidine(2 mg/kg) also prevented MIA-induced increase in the RANKL and OPG levels in the serum. Histopathological analysis showed that rilmenidine was protective on joint capsule and matrix.
Conclusion: Our results suggest that rilmenidine showed the antinociceptive effect on MIA-induced OA via improving bone turnover.
Distribution and ecological status of alien fish species in the Karun River Basin, Iran
Authors: Shahraki Mojgan Zare , Keivany Yazdan , Dorche Eisa Ebrahimi , Blocksom Karen , Bruder Andreas , Flotemersch Joseph 
Published: November 2022

Abstract
We assessed the distribution of alien fishes in one of the most ecologically and economically important aquatic ecosystems in Iran, the Karun River Basin. Fish samples were collected from 35 sites in the Karun River Basin during the low-flow period from November to December 2018. We documented the occurrence of 37 species of fishes from nine orders and 14 families. Amongst these, 10 species were alien (986 individuals; 15.7%). The relative abundance of native, endemic, and alien species estimated as 54.8%, 29.5% and 15.7%, respectively. Total length of alien species ranged from 0.4 to 25.5 cm and their total weight from 0.17 to 350 g. The ten alien species belonged to seven families including, Cyprinidae, Cichlidae, Xenocyprididae, Gobiidae, Poeciliidae, Gobionidae, and Salmonidae. Carassius gibelio, Oreochromis aureus, and Coptodon zillii were found to be the most abundant alien species in the downstream region. We applied redundancy analysis (RDA) as a direct stressor method to identify the extent of changes in alien fish assemblages with environmental parameters. The first two axes (RDA1 and RDA2) accounted for 36.24% and 25.33% of the variation of five alien species, respectively. Altitude, depth, electrical conductivity, water temperature, turbidity, dissolved oxygen, and river width were the most significant parameters affecting the distribution of alien species. Our results propose that management practices are needed in the downstream sections of the Basin to recover native and endemic species. Monitoring of presence and extent of alien species is a key to measuring the success of these efforts.
Stateful Layered Chain Model to Improve the Scalability of Bitcoin
Authors: Elwi Dalia , Abu-Elnasr Osama , Tolba A. S. , Elmougy Samir 
Published: November 2022

Abstract
Bitcoin is a digital cryptocurrency which had become the focus of scientific research in the modern era. Blockchain is the underlying technology of Bitcoin because of its decentralization, transparency, trust-less, and immutability features. However, blockchain can be considered the cause of Bitcoin scalability issues especially storage. Nodes in Bitcoin network need to store the full blockchain to validate transactions. By time, the blockchain size will be extremely huge. So, the full nodes will prefer to leave the network, and this leads to the blockchain being centralized and trusted. Therefore, security will be adversely affected. In this paper, we propose a Stateful Layered Chain Model which is based on storing accounts’ balances to reduce the size of the Bitcoin blockchain. This model changes the structure of the traditional blockchain from blocks to layers. The experimental results demonstrated that the proposed model reduces the size of blockchain by about 50.6%. Imlicitly, the transaction throughput can also be nearly doubled.
Hematologic and serum biochemical characteristics of rare minnow (Gobiocypris rarus) in two different culture environments
Authors: Su Liangxia , Qiu Ning , Hou Miaomiao , Xu Chunsen , Xiong Xiaoqin , Wang Jianwei 
Published: November 2022

Abstract
Hematological and serum biochemical parameters are essential for the evaluation of both structural and function status of fish species due to the involvement of culture environment and sex. To obtain a basic knowledge about the variation in hematological and serum biochemical parameters of conventional (CV) and specific pathogen-free (SPF) rare minnow (Gobiocypris rarus) with respect to sex, blood samples collected from 720 healthy and adult rare minnow (♀:♂=1:1) are analyzed by using standard methods and techniques. The results indicated that hemoglobin (Hb), hematocrit (HCT) and mean corpuscular volume (MCV) values in female CV rare minnow were 82.4 ± 9.5 g/L, 41.6 ± 8.4%, 408.3 ± 81.6 fl, which were significantly lower than those in SPF rare minnow (p < 0.05). Alkaline phosphatase (ALP) differed significantly between these two cultured rare minnows (p < 0.05), and no significant differences were observed in other serum biochemical parameters (p > 0.05). In addition, MCV values of female rare minnow are significantly higher than male individuals (p > 0.05). These data demonstrated that hematological and serum biochemical parameters of rare minnow were affected by culture environment and sex, and that a clean culture environment could contribute to the health physiological status of rare minnow.
Prognostic value and dynamic change characteristics of cytokines in Severe fever with thrombocytopenia syndrome
Authors: Lv Dongmei , Meng Tao , Zhang Luwen , Zuo Yan , Xu Yuanhong 
Published: November 2022

Abstract
Background: Severe fever with thrombocytopenia syndrome (SFTS) is a serious infectious disease that has received widespread attention. This study focuses on the predictive value and trend of cytokines in SFTS.
Methods: Information of 128 SFTS patients retrospectively collected from January 2019 to January 2022 from a large general hospital in Anhui Province, China. Multivariate Cox model was used to identify predictors of death, ROC curve was used to calculate the value of predictors. Linear regression analysis was used to explore the correlation between cytokines and viral titers. Dynamic changes in cytokines to demonstrate therapeutic significance.
Results: Of the 128 patients diagnosed with SFTS, 34 died during the course of the disease. Cytokines were significantly higher in the non-survival group than the survival group, while CD4 and CD8 counts were lower in the non-survival group. Multivariate Cox analysis showed that TNF-α was an independent predictor of death (p = 0.038, HR = 12.78) with an AUC value of 0.901. TNF-α, IL-6, IL-10 and IL-8 were correlated with viral titer (p < 0.001, 0.5 < r < 1). Different cytokines showed different trends in the course of treatment. High dose gamma-globulin shock therapy combined with ribavirin can effectively reduce the level of some cytokines (IL-6, IL-2R, IL-10).
Conclusion: TNF-α is an independent predictor of SFTS death outcome and has excellent predictive value. There was a significant correlation between TNF-α, IL-6, IL-10, IL-8 and virus titer. Gamma-globulin shock therapy combined with ribavirin can control cytokine storm to some extent and provide new ideas for clinical treatment.
Composition and Provenance of Beach Sands in La Graciosa, Lanzarote, Fuerteventura and Gran Canaria Islands (Eastern Canary Islands, Spain): a Review
Authors: Mangas José , Espino Emma Perez-Chacon 
Published: November 2022

Abstract

In this work, a review of petrographic studies in recent decades on 50 sandy beach samples of the eastern Canary Islands (La Graciosa, Lanzarote, Fuerteventura and Gran Canaria) has been carried out in order to know the composition and provenance of the sand grains. The sandy samples are intertidal and most are related to beach-dune systems with coastal desert and arid climates. Thus, these samples contain different proportions of calcareous bioclast, intraclast and volcaniclast grains, with the latter coming from different source areas associated with the building stages of the islands (shield, decline, erosive and rejuvenation). In general, the calcareous bioclasts are mostly fragments of red coralline algae and molluscs, with the appearance of occasional grains of echinoderms, foraminifera and bryozoans. The lithoclasts are essentially fragments of ultramafic and mafic alkaline rocks (basanite and basalt) from the shield and rejuvenation stages and to a lesser extent trachytic-phonolitic felsic rocks from the decline stage, while there are few mafic (olivine, clinopyroxene, Fe-Ti oxide, and amphibole) and felsic (feldspar) mineral grains. The intraclasts are fragments of coastal sedimentary rocks such as beachrocks, eolianites, and paleosols. The abundance percentages depend on geographic and geological factors in each coastal zone. In general, the beaches studied in La Graciosa, Lanzarote and Fuerteventura contain higher percentages of bioclasts than lithoclasts. However, volcaniclasts are more abundant than bioclasts in Gran Canaria, and sand grains of trachytic-phonolitic composition appear only on this island. Finally, urban and semi-urban beaches were found to have fewer bioclasts than natural ones.
Sinusoidal RF Simulations for Optimized Electroporation Protocols
Authors: Milestone W. , Hu Q. , Garner A. L. , Joshi R. P. 
Published: November 2022

Abstract

Protocols surrounding electroporation have long been based on trapezoidal pulsing of biological cells. Here, we revisit cellular electroporation for bio-medical applications, including tumor treatment, based on a self-consistent electro-thermal analysis with sinusoidal RF excitation. Predictions for the evolution of pores and their surface angular distribution, as well as potential heating and temperature increases, are given. Our results show an optimum frequency range from 5–7 MHz to achieve increased mass transport without detrimental heating in Jurkat cells. Through parametrized frequency sweeps, this work establishes potential optimized regimes that could guide experimental and clinical protocols. More significantly, the optimal frequency for porating healthy B-cells is predicted to be ~ 2.5 MHz, with almost no poration at 7 MHz. This opens up the exciting possibility for treating malignant tissue with a well-tuned optimal frequency range for bioeffects, while minimizing deleterious effects on healthy cells and tissues.
Evaluation of different dietary polyphenols on conformational, functional changes and lycopene-loaded nanocarriers of walnut protein isolate
Authors: Wang Yuzhen , Chen Yu , Lv Jiao , Li Chang , Wang Fengjun 
Published: November 2022

Abstract
This study investigated the effects of the interaction of walnut protein isolate (WPI) with epigallocatechin gallate (EGCG), chlorogenic acid (CLA), (+)-catechin (CA), and ellagic acid (EA) on the structural and functional properties of proteins by a free radical grafting method. The results for polyphenol binding equivalents and content of free amino and sulfhydryl groups as well as those from sodium dodecyl sulfate‒polyacrylamide gel electrophoresis confirmed the covalent interaction between WPI and the polyphenols. The binding capacities of the mixtures and conjugates were as follows: WPI-EGCG > WPI-CLA > WPI-CA > WPI-EA. Fourier transform infrared spectroscopy (FTIR) and fluorescence spectrum analysis identified changes in the protein structure. The conjugation process obviously increased the polyphenols’antioxidant properties and the surface hydrophobicity was substantially reduced. WPI-EGCG conjugates had the best functional properties, followed by WPI-CLA, WPI-CA, and WPI-EA. Lycopene (LYC) was loaded into nanocarriers by WPI-EGCG self-assembly. These results indicated that WPI-polyphenol conjugates can be utilized to develop food-grade delivery systems to protect chemically lipophilic bioactive compounds.