bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recruitment of a splicing factor to the nuclear lamina for its inactivation
Authors: Vester Karen , Preußner Marco , Holton Nicole , Feng Suihan , Schultz Carsten , Heyd Florian , Wahl Markus C. 
Published: July 2022

AbstractPrecursor messenger RNA splicing is a highly regulated process, mediated by a complex RNA-protein machinery, the spliceosome, that encompasses several hundred proteins and five small nuclear RNAs in humans. Emerging evidence suggests that the spatial organization of splicing factors and their spatio-temporal dynamics participate in the regulation of splicing. So far, methods to manipulate the spatial distribution of splicing factors in a temporally defined manner in living cells are missing. Here, we describe such an approach that takes advantage of a reversible chemical dimerizer, and outline the requirements for efficient, reversible re-localization of splicing factors to selected sub-nuclear compartments. In a proof-of-principle study, the partial re-localization of the PRPF38A protein to the nuclear lamina in HEK293T cells induced a moderate increase in intron retention. Our approach allows fast and reversible re-localization of splicing factors, has few side effects and can be applied to many splicing factors by fusion of a protein tag through genome engineering. Apart from the systematic analysis of the spatio-temporal aspects of splicing regulation, the approach has a large potential for the fast induction and reversal of splicing switches and can reveal mechanisms of splicing regulation in native nuclear environments.
A diagnostic classifier for gene expression-based identification of early Lyme disease
Authors: Servellita Venice , Bouquet Jerome , Rebman Alison , Yang Ting , Samayoa Erik , Miller Steve , Stone Mars , Lanteri Marion , Busch Michael , Tang Patrick , Morshed Muhammad , Soloski Mark J. , Aucott John , Chiu Charles Y. 
Published: July 2022

Abstract
Background
Lyme disease is a tick-borne illness that causes an estimated 476,000 infections annually in the United States. New diagnostic tests are urgently needed, as existing antibody-based assays lack sufficient sensitivity and specificity.

Methods
Here we perform transcriptome profiling by RNA sequencing (RNA-Seq), targeted RNA-Seq, and/or machine learning-based classification of 263 peripheral blood mononuclear cell samples from 218 subjects, including 94 early Lyme disease patients, 48 uninfected control subjects, and 57 patients with other infections (influenza, bacteremia, or tuberculosis). Differentially expressed genes among the 25,278 in the reference database are selected based on ≥1.5-fold change, ≤0.05 p value, and ≤0.001 false-discovery rate cutoffs. After gene selection using a k-nearest neighbor algorithm, the comparative performance of ten different classifier models is evaluated using machine learning.

Results
We identify a 31-gene Lyme disease classifier (LDC) panel that can discriminate between early Lyme patients and controls, with 23 genes (74.2%) that have previously been described in association with clinical investigations of Lyme disease patients or in vitro cell culture and rodent studies of Borrelia burgdorferi infection. Evaluation of the LDC using an independent test set of samples from 63 subjects yields an overall sensitivity of 90.0%, specificity of 100%, and accuracy of 95.2%. The LDC test is positive in 85.7% of seronegative patients and found to persist for ≥3 weeks in 9 of 12 (75%) patients.

Conclusions
These results highlight the potential clinical utility of a gene expression classifier for diagnosis of early Lyme disease, including in patients negative by conventional serologic testing.
Pediatric Amplification: A Proposed Protocol for In-Person Hearing Aid Fittings and Virtual Follow-Ups
Authors: Petrarca Kirsten A. , Worthington Megan 
Published: July 2022

Background:
Children with hearing loss and their families face many financial and logistical barriers to accessing audiological care. At Rush University's Student Community Outreach Program of Excellence (SCOPE), a pediatric hearing loss outreach program is under development to address and overcome those barriers through in-person hearing aid fittings and virtual follow-up appointments.

Objectives:
The goal of this clinical focus article was to develop a proposed protocol for SCOPE's pediatric hearing loss outreach program that would detail the use of a bimodal model of service delivery for pediatric amplification services. This clinical focus article provides a general description of the proposed protocol.

Method:
The proposed protocol was developed as a guideline for future service delivery within SCOPE's pediatric hearing loss outreach program. Categories and details within the protocol were derived from previously published protocols and clinically relevant research.

Results:
The final protocol is composed of six sections, which detail the rationale and target population, necessary equipment, procedures for in-person hearing aid fittings, procedures for virtual follow-ups, outcome measures, and schedule of appointments.

Discussion:
On the national level, access to audiological care for pediatric patients and their families is restricted by both financial and logistical barriers. A telehealth model of service delivery has been shown to be effective in providing high-quality patient care while addressing these barriers. A clinical program using a bimodal model of service delivery will be implemented to address these barriers in Chicago, Illinois. Future investigation is required to monitor the efficacy of the program and develop program-specific materials.
The Impact of Daily Hours of Sound Therapy on Tinnitus Relief for People With Chronic Tinnitus: A Randomized Controlled Study
Authors: Jin In-Ki , Choi Soon-Je , Ku Minseung , Sim YeonWoo , Lee TaeRim 
Published: July 2022

Purpose:
Tinnitus is defined as the perception of sounds in the absence of extrinsic sound stimuli. Sound therapy is an option for tinnitus rehabilitation, which aims to mitigate the functional and emotional effects of tinnitus. Several studies have reported that a longer duration of sound therapy may result in a greater tinnitus relief effect. However, the relationship between the daily hours of sound therapy and the tinnitus relief effect remains unclear. Thus, we aimed to evaluate the tinnitus relief effect according to the time spent in daily performing sound therapy.

Method:
Fifty-eight chronic tinnitus participants were randomly assigned to three groups according to their daily hours of sound therapy (1-hr, 3-hr, and 5-hr groups), and mixing point-based sound therapy, which adjusts the volume of the external stimulus to blend with the participant's tinnitus sound, was administered for 3 months. The effectiveness of sound therapy was determined based on changes in the tinnitus loudness level, visual analog scale for loudness score, and the Korean version of the Tinnitus Primary Function Questionnaire (K-TPFQ) score between the baseline and 3-month time points.

Results:
Longer daily sound therapy hours were associated to a greater effect of sound therapy in the tinnitus loudness level and the total K-TPFQ scores, including the emotion and hearing subcategories. Particularly, the 3-hr and 5-hr groups showed a greater tinnitus relief than the 1-hr group.

Conclusion:
An increase in the daily sound therapy hours may increase the possibility of improving tinnitus relief effects.
Event‐based motion segmentation of small objects in the wild
Authors: Sun Zhezheng , Hou Chuantong , Wang Longguang , Deng Xinpu , Li Miao 
Published: July 2022

AbstractEvent‐based cameras are sensitive to brightness changes and can capture rich temporal information with very high temporal resolution, which has great potential for motion segmentation of moving objects. Under static background, events are only triggered by motion of objects, thereby moving objects can be easily segmented. However, in many real‐world applications, events are also triggered by the motion of camera or background and submerge the ones corresponding to moving objects. In this letter, an event‐based motion segmentation method to segment moving small objects in events obtained from the wild is proposed. First, motion estimation is performed to align the events triggered by the background. Then, candidate events corresponding to moving objects or moving backgrounds are detected. Finally, motion information is adopted to segment the events of moving small objects from the ones triggered by the background. In addition, the first dataset for event‐based motion segmentation of small objects is developed. Experimental results demonstrate the effectiveness of this method and show that this method can achieve robust motion segmentation of small moving objects in the wild.
Physical properties and mini-outburst of 64P/Swift-Gehrels
Authors: Xu R. Q. , Shi J. C. , Ma Y. H. , Li F. , Yuan Y. 
Published: July 2022

Aims. We present secular multiwavelength broadband photometry, spanning over 93 days, performed at the Yaoan High Precision Telescope on comet 64P/Swift-Gehrels in its 2018–2019 apparition. Our aim is to study its dust activity, coma properties, and the accidentally discovered mini-outburst.
Methods. We used aperture photometry to measure the Afρ value and correct the back-scattering effect based on phase angle. We deployed azimuthally averaged image enhancement methods to identify the coma morphology and the outburst. Secular color measurement was also conducted on the comet.
Results. We identified a −0.5 mag mini-outburst around January 3, 2019, with coma morphology study showing an obvious dust jet feature strengthened during the outburst. The maximum A(0)fρ value of 64P/Swift-Gehrels recorded is 313 cm at the top of the fitted curve and 334 cm at the outburst event. Both volatile-driven pressure mechanisms and nonvolatile-driven mechanisms could be the major explanation for the outburst. We obtain a mean color B − V = 0.68 ± 0.03 and V − R = 0.08 ± 0.01. The B − V color is similar to the mean value for Jupiter Family comets, but the V − R color turns out to be bluest among all the recorded short-period comets. The cause of this anomaly remains unknown.
Wie wurden Thuja & Co. zu Arzneien für Impffolgen?
– Ein historischer Überblick bis in die
Gegenwart
Authors: Zauner Bernhard 
Published: July 2022

ZusammenfassungImpfungen werden in Medizin, Gesellschaft und Politik schon immer kontrovers
diskutiert und sind ein Thema, das die Gemüter erhitzt. Auch innerhalb
der homöopathisch arbeitenden Ärzteschaft gab es in den letzten
200 Jahren verschiedene Strömungen und Entwicklungen.90% der Impfungen werden von niedergelassenen Ärzten
durchgeführt, diese sind daher zur Aufklärung und Beratung der
Patienten verpflichtet. Als Leitlinien und zur Orientierung bei Impffragen
stehen aktuelle Empfehlungen der jeweiligen Gesundheitsministerien zur
Verfügung.Bestimmte homöopathische Arzneien genießen den Status, als
besonders für Impffolgen geeignet zu sein.
The Physiological and Cardiologic Effects of Long Video Gaming
Sessions in Adult Males
Authors: Krarup Kasper Bygum , Rantanen Jesper Mosgaard , Mørk Morten , Pedersen Inge Søkilde , Christensen Jeppe Hagstrup , Kristensen Søren Risom , Handberg Aase , Krarup Henrik Bygum 
Published: July 2022

AbstractThe effect of long gaming sessions on energy intake, caffeine intake, blood
pressure, heart rate, heart rate variability, and biochemical cardiac injury
markers is unknown. The objective of this exploratory study was to investigate
the changes in healthy male adults during two consecutive 18-hour sedentary
video gaming sessions. Nine participants were enrolled in the study. Energy
intake was noted in food diaries. Heart rate variability was monitored
continuously; blood pressure and cardiac injury markers were measured every
three to six hours. During the 42-hour study, the participants had an energy and
caffeine intake of 8004.9 kcal and 1354.4 mg, respectively. The
participants had a significant decrease in energy intake in the second session
(p=0.01). A strong, negative correlation was found between body mass
index and total energy intake (R=–0.84, p=0.005) and
waist circumference and total energy intake (R=–0.70,
p=0.036) in the first session. No nightly dip in blood pressure or heart
rate was observed. Based on this study, long-term adverse effects of gaming
cannot be ruled out. The non-dip of HR and BP suggests that long gaming sessions
could be detrimental to cardiovascular health long term.
Pleiotropic Benefits of Statins in Cardiovascular
Diseases
Authors: Wasim Rufaida , Ansari Tarique Mahmood , Ahsan Farogh , Siddiqui Mohammad Haris , Singh Aditya , Shariq Mohammad , Parveen Saba 
Published: July 2022

AbstractIn 1976, Japanese microbiologist Akira Endo discovered the first statin as a
product of the fungus Penicillium citrinum that inhibited the activity of
3-hydroxy-3-methylglutaryl coenzyme A reductase. Their primary mode of action is
to lower the blood cholesterol by decreasing hepatic cholesterol production,
which upregulates hepatic low-density lipoprotein (LDL) receptors and increases
LDL-cholesterol clearance. In addition to cholesterol lowering, statins inhibit
other downstream products of the mevalonate pathway, causing the so-called
pleiotropic effects. As a result of their pleiotropic effects statins modulate
virtually all known processes of atherosclerosis and have beneficial effects
outside the cardiovascular system Statins inhibit the post-translational
prenylation of small GTP-binding proteins such as Rho, Rac, as well as their
downstream effectors such as Rho kinase and nicotinamide adenine dinucleotide
phosphate oxidases since they suppress the synthesis of isoprenoid intermediates
in the cholesterol biosynthetic pathway altering the expression of endothelial
nitric oxide synthase, the stability of atherosclerotic plaques, production of
proinflammatory cytokines, reactive oxygen species, platelet reactivity,
development of cardiac hypertrophy and fibrosis in cell culture and animal
experiments. Inhibition of Rho and Rho-associated coiled-coil containing protein
kinase (ROCK), has emerged as the principle mechanisms underlying the
pleiotropic effects of statins. However, the relative contributions of statin
pleiotropy to clinical outcomes are debatable and difficult to measure because
the amount of isoprenoid inhibition by statins corresponds to some extent with
the amount of LDL-cholesterol decrease. This article examines some of the
existing molecular explanations underlying statin pleiotropy and discusses if
they have clinical relevance in cardiovascular diseases.
Biocomponentes à base de hidroxiapatita: Influência da esterilização na resistência mecânica
Authors: Tagliari Ivânio , Lerner Alan Menegaz , Severo Antônio Lourenço , Israel Charles Leonardo 
Published: July 2022

Resumo
Objetivo O objetivo deste estudo foi avaliar a influência da esterilização na resistência mecânica à compressão e flexão de biocomponentes à base de hidroxiapatita obtida a partir de osso bovino liofilizado e sua associação com quitosana.
Métodos O osso bovino liofilizado foi processado em partículas de 100 μm e misturado à quitosana em proporção de 50% de seu peso. A mistura foi acondicionada em moldes metálicos para preparo dos espécimes e esterilizada a 127°C em autoclave para posterior experimentação. Os espécimes foram submetidos a ensaios de compressão e flexão seguindo a norma 5833 da International Organization for Standardization (ISO); os espécimes eram blocos cilíndricos de 6 × 12 mm (para ensaios de compressão) e placas de 75 × 10 × 3,3 mm (para ensaios de flexão). As amostras foram divididas em quatro grupos de 20 espécimes cada, sendo 10 para ensaios de compressão e 10 para ensaios de flexão. Três grupos foram esterilizados (por autoclavagem, raios gama e óxido de etileno), enquanto o quarto grupo (controle) não foi. Os testes mecânicos obtidos nos diferentes processos de esterilização foram comparados por análise de variância (ANOVA, p < 0,05) seguido pelo teste de comparação múltipla de médias de Tukey, com intervalo de confiança de 95%.
Resultados Os espécimes apresentaram resistências médias à compressão de 10,25 MPa para o grupo de controle e 3,67 MPa, 9,65 MPa e 9,16 MPa após esterilização com óxido de etileno, raios gama e autoclavagem, respectivamente. Os resultados do teste de flexão mostraram uma resistência média de 0,40 MPa no grupo de controle, e 0,15 MPa, 0,17 MPa e 0,30 MPa após esterilização com óxido de etileno, raios gama e autoclavagem, respectivamente. A compressão máxima observada no grupo esterilizado com óxido de etileno foi estatisticamente diferente à obtida no grupo de controle (p = 0,0002), esterilizado com raios gama (p = 0,0003) e autoclavado (p = 0,0006). A flexão máxima dos espécimes esterilizados com raios gama foi estatisticamente diferente à observada no grupo de controle (p = 0,0245). No entanto, a resistência à flexão foi baixa em todos os espécimes.
Conclusão A esterilização em autoclave não foi associada a diferenças estatisticamente significativas nos testes de compressão ou flexão. Assim, a autoclave foi a melhor opção de esterilização para os biocomponentes à base de hidroxiapatita neste estudo.
Há diferença na força de compressão interfragmentar entre parafusos com rosca total ou parcial? Resultados de um estudo biomecânico experimental piloto
Authors: Okoro Tosan , Landgren Marcus , Afenu Edem , Russow Gabriele , Wulsten Dag , Heyland Mark 
Published: July 2022

Resumo
Objetivo Este estudo avaliou diferenças entre parafusos com rosca total ou parcial na resistência à compressão interfragmentar inicial. Nossa hipótese era de que haveria maior perda de resistência à compressão inicial com o parafuso de rosca parcial.
Métodos Uma linha de fratura oblíqua de 45 graus foi criada em amostras de osso artificial. O primeiro grupo (TOTAL, n = 6) foi fixado com um parafuso de 3,5 mm de rosca total, enquanto o segundo grupo (PARCIAL, n = 6) usou um parafuso de 3,5 mm de rosca parcial. Avaliamos a rigidez à torção em ambas as direções de rotação. Os grupos foram comparados com base nos seguintes parâmetros biomecânicos: momento de rigidez-ângulo, momento de rigidez-tempo, momento de torção máxima (carga de falha) e força de compressão calibrada com base na medida do sensor de pressão.
Resultados Depois da perda de uma amostra PARCIAL, não foram observadas diferenças estatisticamente significativas na força de compressão calibrada entre os 2 grupos [mediana (intervalo interquartil)]: TOTAL: 112,6 (10,5) N e PARCIAL: 106,9 (7,1) N, com p = 0,8 segundo o teste U de Mann-Whitney). Além disso, após a exclusão de 3 amostras para testes mecânicos (TOTAL, n = 5, PARCIAL, n = 4), não foram observadas diferenças estatisticamente significativas entre os construtos TOTAL e PARCIAL quanto ao momento de rigidez-ângulo, momento de rigidez-tempo ou momento de torção máxima (carga de falha).
Conclusão Não há diferença aparente na força de compressão inicial (força de compressão ou rigidez do construto ou carga de falha) com o uso de parafusos de rosca total ou parcial neste modelo biomecânico em osso artificial de alta densidade. Parafusos de rosca total podem, portanto, ser mais úteis no tratamento de fraturas diafisárias. Mais pesquisas são necessárias sobre o impacto em modelos ósseos osteoporóticos ou metafisários de menor densidade e avaliação do significado clínico.
Técnica de reconstrução do pé com prótese de calcâneo feita sob medida em câncer primário – Relato de caso
Authors: Gowda Aditya K. S. , Dhingra Mohit , Vathulya Madhubari , Seenivasagam Rajkumar K. 
Published: July 2022

ResumoO pé é um local incomum para tumores ósseos, e compreende cerca de 3% de todos os tumores esqueléticos, em especial ao redor do calcâneo. A cirurgia radical cria um vazio no pé, o que afeta de forma negativa a capacidade de resgate do membro. As cirurgias de reconstrução do calcâneo não são comumente realizadas por causa da instabilidade da prótese, defeito de partes moles, e consequente possibilidade de insucesso pós-operatório. Assim, apresentamos aqui um caso raro de sarcoma sinovial originário da bainha do tendão tibial posterior com acometimento secundário do osso calcâneo. Considerando as experiências prévias de diferentes cirurgiões, projetamos uma prótese sob medida com modificações relevantes.
Two approaches for the passive charge management of contactless test masses
Authors: Wang S , Saraf S , Lipa J , Yadav D , Buchman S 
Published: July 2022

Abstract
Free floating test masses (TM) of inertial reference instruments accumulate charge mainly through the triboelectric effect during separation from their housings and, if in the space environment, from cosmic radiation. These charges will degrade the accuracy of high sensitivity accelerometers and drag-free sensors. We demonstrate in ground testing two passive bipolar charge management systems using photoelectrons emitted from gold coated surfaces under illumination by ultraviolet light emitting diodes (UV-LEDs) with 255 nm, 275 nm, and 295 nm central wavelengths. The first method uses fast photoelectrons, generated by two 255 nm UV-LEDs with adjustable-intensity (through fine-tuning of their excitation currents) and illuminating the TM and its housing respectively. A second technique uses slow photoelectrons generated by one UV-LED, of either 275 nm or 295 nm, directed at the TM. Fast and slow electrons are defined as having kinetic energies after photoemission above and below

e
V

T
M

max

, where

V

T
M

max

is the maximum allowable potential required for normal operation of the TM. In its optimized configurations and following an exposure of <30 s to UV, the fast-photoelectron control system converges to zero TM potential from ±1 V with a drift of ≈1.5 mV/day. The slow-photoelectron system requires about 5 min to converge to zero TM potential from 100 mV, with a similar drift of ≈2.0 mV/day. For reference,

V

T
M

max

≅
80

m
V

for the LISA and LISA pathfinder sensors. Insight into the slow photoelectrons charge control method is provided by our simple Gaussian model of the work function for gold films (that approximates their quasi-triangular measured shape): 4.6 eV center, 0.2 eV standard deviation, and 4.30 eV and 4.90 eV cutoffs. The longest wavelength (lowest energy) photoemission is obtained with the 295 nm LED (11 nm FWHM) at about 285 nm (4.35 eV), where only about 3.5% of the photons have an energy above the photoelectric threshold. While the two-source UV fast photoelectron method is a modification of the LISA Pathfinder approach, the slow photoelectron method is a new passive charge management technique. These two passive techniques were developed for instruments without sensing and activation systems. For instruments with electric fields surrounding the TM and more complex geometries additional adaptations are required.