bluuebunny/crossref_metadata_2025_split huggingface dataset:
Healthcare use among people with diabetes mellitus in Europe: a population-based cross-sectional study
Authors: Fuentes-Merlos Álvaro , Quesada-Rico José Antonio , Reina Raul , Orozco-Beltrán Domingo 
Published: November 2022

ObjectiveThis study aimed to determine the association of health determinants, lifestyle and socioeconomic variables on healthcare use in people with diabetes in Europe.DesignA cross-sectional study was conducted using data from the European Health Interview Survey wave 2 (ie, secondary analysis).SettingThe sample included data from 25 European countries.ParticipantsThe sample included 16 270 patients with diabetes aged 15 years or older (49.1% men and 50.9% women).ResultsThe survey data showed that 58.2% of respondents had seen their primary care physician in the past month and 22.6% had been admitted to the hospital in the past year. Use of primary care was associated with being retired (prevalence ratio (PR) 1.13, 95% CI 1.07 to 1.19) and having very poor self-perceived health (PR 1.80, 95% CI 1.51 to 2.15), long-standing health problems (PR 1.14, 95% CI 1.04 to 1.24), high blood pressure (PR 1.06, 95% CI 1.03 to 1.10) and chronic back pain (PR 1.07, 95% CI 1.04 to 1.11). Hospital admission was associated with very poor self-perceived health (PR 3.03, 95% CI 2.14 to 4.31), accidents at home (PR 1.54, 95% CI 1.40 to 1.69), chronic obstructive pulmonary disease (COPD) (PR 1.34, 95% CI 1.22 to 1.47), high blood pressure (PR 1.08, 95% CI 1.01 to 1.17), chronic back pain (PR 0.91, 95% CI 0.84 to 0.98), moderate difficulty walking (PR 1.33, 95% CI 1.21 to 1.45) and severe difficulty walking (PR 1.67, 95% CI 1.51 to 1.85).ConclusionsIn the European diabetic population, the high cumulative incidences of primary care visits and hospital admissions are associated with labour status, alcohol consumption, self-perceived health, long-standing health problems, high blood pressure, chronic back pain, accidents at home, COPD and difficulty walking.
Aspirin versus aggressive antiplatelet therapy for acute carotid stenting plus thrombectomy in tandem occlusions: ETIS Registry results
Authors: Marnat Gaultier , Finistis Stefanos , Moreno Ricardo , Sibon Igor , Pop Raoul , Mazighi Mikaël , Clarençon Frédéric , Rosso Charlotte , Dargazanli Cyril , Darcourt Jean , Olivot Jean-Marc , Boulouis Gregoire , Janot Kevin , Moulin Solène , Bourcier Romain , Consoli Arturo , Richard Sébastien , Arquizan Caroline , Vannier Stephane , Richter Sebastian , Gentric Jean-Christophe , Papagiannaki Chrisanthi , Naggara Olivier , Eker Omer F , Lapergue Bertrand , Caroff Jildaz , Gory Benjamin 
Published: November 2022

BackgroundPatients treated with acute carotid stenting (CAS) may have higher odds of a favorable outcome than those treated without CAS during thrombectomy in tandem occlusions. Antiplatelet therapy is associated with CAS to avoid stent thrombosis, which occurs in around 20% of patients and negatively impacts outcomes. In this study we compared two antiplatelet strategies in tandem occlusion strokes treated with CAS and intracranial thrombectomy in clinical practice.MethodsThe Endovascular Treatment in Ischemic Stroke Registry is an ongoing prospective observational study involving 21 comprehensive stroke centers performing thrombectomy in France. We analyzed patients with atherosclerotic tandem occlusions treated with acute CAS and intracranial thrombectomy who received at least one antiplatelet agent. Aggressive antiplatelet therapy included oral or intravenous glycoprotein (GP) IIb/IIIa or P2Y12 inhibitors. The primary outcome was cervical carotid artery patency at day 1 imaging follow-up.ResultsAmong the 187 included patients, 124 (66.3%) received aspirin alone and 63 (33.7%) received aggressive antiplatelet therapy. There was no significant difference regarding safety outcomes, especially in symptomatic intracerebral hemorrhage, parenchymal hematoma, and procedural complications. There was a significantly higher rate of carotid stent patency at day 1 in the aggressive antiplatelet therapy group (81.7% vs 97.1%, aOR 17.49, 95% CI 1.10 to 277.2, p=0.042). Odds of favorable functional outcome (90-day modified Rankin Scale score 0–2) were similar between the groups (OR 3.04, 95% CI 0.64 to 14.25, p=0.158).ConclusionsIn tandem occlusions treated with CAS plus thrombectomy, an aggressive antiplatelet regimen was associated with an increased rate of carotid stent patency at day 1 without safety concerns. Randomized trials are warranted to confirm these findings.
Desire for hastened death in advanced cancer: cross-sectional study in China
Authors: Liu Feng , Peng Wanglian , Zhou Ran , Huang Xufen , Yang Hui , Wen Minni , zhang Lemeng , Tong Fei , Yang Desong , Jiang Ling , Yi Lili , Liu Xiaohong 
Published: November 2022

ObjectiveTo evaluate the incidence of desire for hastened death (DHD) among patients with advanced cancer and to identify factors associated with DHD.MethodsThis was a cross-sectional study of 227 patients with advanced cancer in Hunan Cancer Hospital. The patients were assessed using Chinese version of the Schedule of Attitudes toward Hastened Death, Karnofsky Performance Scale, Quality of Life (QOL), MD Anderson Symptom Inventory and Patient Health Questionnaire Depression Module-9.ResultsThe number of patients with or without DHD were 71 (31.3%) and 156 (68.7%), respectively. Follow-up visits and average and high QOL were protective factors for DHD; severely disturbed sleep, symptoms that severely interfered with mood, and symptoms that severely interfered with relations with other people were risk factors for DHD.ConclusionsThe incidence of the DHD in patients with advanced cancer at home is high. Those who have low QOL, severely disturbed sleep, symptoms that severely interfered with mood, or symptoms that severely interfered with relations with other people should be paid attention to. These data provide a theoretical basis for the early detection and diagnosis of the desire to accelerate death of patients with advanced cancer.
Quality of life outcomes in children after surgery for Hirschsprung disease and anorectal malformations: a systematic review and meta-analysis
Authors: Oltean Irina , Hayawi Lamia , Larocca Victoria , Bijelić Vid , Beveridge Emily , Kaur Manvinder , Grandpierre Viviane , Kanyinda Jane , Nasr Ahmed 
Published: November 2022

BackgroundNo systematic review and meta-analysis to date has examined multiple child and parent-reported social and physical quality of life (QoL) in pediatric populations affected by Hirschsprung’s disease (HD) and anorectal malformations (ARM). The objective of this systematic review is to quantitatively summarize the parent-reported and child-reported psychosocial and physical functioning scores of such children.MethodsRecords were sourced from the CENTRAL, EMBASE, and MEDLINE databases. Studies that reported child and parent reported QoL in children with HD and ARM, regardless of surgery intervention, versus children without HD and ARM, were included. The primary outcome was the psychosocial functioning scores, and the secondary outcomes were the presence of postoperative constipation, postoperative obstruction symptoms, fecal incontinence, and enterocolitis. A random effects meta-analysis was used.ResultsTwenty-three studies were included in the systematic review, with 11 studies included in the meta-analysis. Totally, 1678 total pediatric patients with HD and ARM underwent surgery vs 392 healthy controls. Pooled parent-reported standardized mean (SM) scores showed better social functioning after surgery (SM 91.79, 95% CI (80.3 to 103.3), I2=0). The pooled standardized mean difference (SMD) showed evidence for parent-reported incontinence but not for constipation in children with HD and ARM after surgery that had a lower mean QoL score compared with the normal population (SMD −1.24 (-1.79 to –0.69), I2=76% and SMD −0.45, 95% CI (−1.12 to 0.21), I2=75%). The pooled prevalence of child-reported constipation was 22% (95% CI (16% to 28%), I2=0%). The pooled prevalence of parent-reported postoperative obstruction symptoms was 61% (95% CI (41% to 81%), I2=41%).ConclusionThe results demonstrate better social functioning after surgery, lower QoL scores for incontinence versus controls, and remaining constipation and postoperative obstruction symptoms after surgery in children with HD and ARM.
Preparation of pretreated sugarcane bagasse and chitosan bio-composite for the adsorption of phosphate from aqueous media
Authors: Manyatshe Alusani , Cele Zamani E.D. , Balogun Mohammed O. , Nkambule Thabo T.I. , Msagati Titus A.M. 
Published: November 2022

In this work, we report on the biosorbent bio-composite consisting of pretreated sugarcane bagasse, chitosan, and epichlorohydrin, which was prepared using the blending method for the adsorption of phosphate from aqueous media. The effect of solvent concentration was investigated, and it was observed that the high solvent concentration significantly enhanced the extraction of cellulose. Moreover, pretreatment enhances the compatibility between the extracted cellulose and chitosan. Furthermore, the addition of epichlorohydrin significantly enhanced the adsorption capacity as well as the stability of the synthesized biosorbent bio-composite. Fourier transform infrared, X-ray photoelectron spectroscopy, scanning electron microscope, X-ray diffraction, and thermogravimetric analysis were used to characterize the prepared bio-composite. Batch studies were conducted to investigate the effectiveness of the bio-composite. The mechanism of adsorption between the biosorbent bio-composite and phosphate was through chemisorption as the data best fitted to the pseudo-second-order kinetic model. The isotherm study showed that the data best fitted to the Freundlich adsorption isotherm model, and the Langmuir adsorption isotherm model showed that the maximum adsorption capacity was 15.26 mg/g. The biosorbent was found to be pH dependent, selective, and recyclable.
Convenient preparation of copper(I) N-heterocyclic carbene complexes under mild conditions
Authors: Lim Meilin Q. , Keske Eric C. 
Published: November 2022

This work describes a new method for the preparation of copper N-heterocyclic carbene (NHC) complexes, which is conducted under air with mild conditions, using bench-stable precursors and a weak base (NEt3). Under optimized conditions, the complexes were prepared in short reaction times of 1 h, giving moderate to good yields after purification by column chromatography. Commonly utilized NHC–HCl salts were found to be applicable reacting with CuCl or CuI giving [(NHC)CuX]-type complexes (X = Cl, I). This study adds to the growing literature in the preparation of catalytically relevant transition metal NHC complexes under mild and operationally simple conditions.
Solitary states in complex networks: impact of topology
Authors: Schülen Leonhard , Mikhailenko Maria , Medeiros Everton S. , Zakharova Anna 
Published: November 2022

AbstractThe dynamical behavior of networked systems is expected to reflect the properties of their coupling structure. Yet, symmetry-broken solutions often occur in symmetrically coupled networks. An example are so-called solitary states where the dynamics of one network node is different from the synchronized rest. Here, we investigate the structural constraints of networks for the appearance of solitary states. By performing a large number of numerical simulations, we find that such states occur with high probability in asymmetric networks, among them scale-free ones. We analyze the structural properties of the networks that support solitary states. We demonstrate that the minimum neighbor node degree of a solitary node is crucial for the appearance of solitary states. Finally, we perform bifurcation analysis of dimension-reduced systems, which confirm the importance of the connectivity of the neighboring nodes.
A global view on the Ordovician stratigraphy of southeastern Europe
Authors: Ferretti Annalisa , Schönlaub Hans Peter , Sachanski Valeri , Bagnoli Gabriella , Serpagli Enrico , Vai Gian Battista , Yanev Slavcho , Radonjić Miloš , Balica Constantin , Bianchini Luca , Colmenar Jorge , Gutiérrez-Marco Juan Carlos 
Published: November 2022

Abstract
The Ordovician documented in southeastern Europe reflects different sedimentary environments, from shallow water to basin, belonging to diverse palaeogeographical domains. Some of these geological sectors and their palaeontological content have been well described for a long time such as the Carnic Alps, which represent one of the most continuous Paleozoic sequences in the world. For some other areas, the quality of the data is variable and the knowledge is less detailed, sometimes with lithostratigraphic units still to be formalized, which also reflects the fragmentary nature of the outcrops. The Ordovician stratigraphy of southeastern Europe with its diverse successions has been revised herein and integrated with new data in an attempt to develop a global scenario for this critical time interval in the evolution of life.
ATM Regulates Differentiation of Myofibroblastic Cancer-Associated Fibroblasts and Can Be Targeted to Overcome Immunotherapy Resistance
Authors: Mellone Massimiliano , Piotrowska Klaudia , Venturi Giulia , James Lija , Bzura Aleksandra , Lopez Maria A. , James Sonya , Wang Chuan , Ellis Matthew J. , Hanley Christopher J. , Buckingham Josephine F. , Cox Kerry L. , Hughes Gareth , Valge-Archer Viia , King Emma V. , Beers Stephen A. , Jaquet Vincent , Jones George D.D. , Savelyeva Natalia , Sayan Emre , Parsons Jason L. , Durant Stephen , Thomas Gareth J. 
Published: November 2022

Abstract

Myofibroblastic cancer-associated fibroblast (myoCAF)–rich tumors generally contain few T cells and respond poorly to immune-checkpoint blockade. Although myoCAFs are associated with poor outcome in most solid tumors, the molecular mechanisms regulating myoCAF accumulation remain unclear, limiting the potential for therapeutic intervention. Here, we identify ataxia-telangiectasia mutated (ATM) as a central regulator of the myoCAF phenotype. Differentiating myofibroblasts in vitro and myoCAFs cultured ex vivo display activated ATM signaling, and targeting ATM genetically or pharmacologically could suppress and reverse differentiation. ATM activation was regulated by the reactive oxygen species–producing enzyme NOX4, both through DNA damage and increased oxidative stress. Targeting fibroblast ATM in vivo suppressed myoCAF-rich tumor growth, promoted intratumoral CD8 T-cell infiltration, and potentiated the response to anti–PD-1 blockade and antitumor vaccination. This work identifies a novel pathway regulating myoCAF differentiation and provides a rationale for using ATM inhibitors to overcome CAF-mediated immunotherapy resistance.

Significance:
ATM signaling supports the differentiation of myoCAFs to suppress T-cell infiltration and antitumor immunity, supporting the potential clinical use of ATM inhibitors in combination with checkpoint inhibition in myoCAF-rich, immune-cold tumors.
MEX3A Mediates p53 Degradation to Suppress Ferroptosis and Facilitate Ovarian Cancer Tumorigenesis
Authors: Wang Cheng-Kai , Chen Tzu-Jou , Tan Grace Y.T. , Chang Fang-Pei , Sridharan Samyuktha , Yu Chen-Hsin Albert , Chang Yen-Hou , Chen Yi-Jen , Cheng Li-Tzu , Hwang-Verslues Wendy W. 
Published: November 2022

Abstract

Epithelial ovarian cancer is a highly heterogeneous and malignant female cancer with an overall low survival rate. Mutations in p53 are prevalent in the major ovarian cancer histotype, high-grade serous ovarian carcinoma (HGSOC), while p53 mutations are much less frequent in other ovarian cancer subtypes, particularly in ovarian clear cell carcinoma (OCCC). Advanced stage OCCC with wild-type (WT) p53 has a worse prognosis and increased drug resistance, metastasis, and recurrence than HGSOC. The mechanisms responsible for driving the aggressiveness of WT p53-expressing ovarian cancer remain poorly understood. Here, we found that upregulation of MEX3A, a dual-function protein containing a RING finger domain and an RNA-binding domain, was critical for tumorigenesis in WT p53-expressing ovarian cancer. MEX3A overexpression enhanced the growth and clonogenicity of OCCC cell lines. In contrast, depletion of MEX3A in OCCC cells, as well as ovarian teratocarcinoma cells, reduced cell survival and proliferative ability. MEX3A depletion also inhibited tumor growth and prolonged survival in orthotopic xenograft models. MEX3A depletion did not alter p53 mRNA level but did increase p53 protein stability. MEX3A-mediated p53 protein degradation was crucial to suppress ferroptosis and enhance tumorigenesis. Consistently, p53 knockdown reversed the effects of MEX3A depletion. Together, our observations identified MEX3A as an important oncogenic factor promoting tumorigenesis in ovarian cancer cells expressing WT p53.

Significance:
Degradation of p53 mediated by MEX3A drives ovarian cancer growth by circumventing p53 tumor suppressive functions, suggesting targeting MEX3A as a potential strategy for treating of ovarian cancer expressing WT p53.
Molecular Monitoring in Endometrial Cancer—Ready for Prime Time?
Authors: Grant Brooke M. , Pugh Trevor J. , Oza Amit M. 
Published: November 2022

Summary
Efforts are under way to define the role of minimally invasive strategies for molecular monitoring and risk stratification in endometrial cancer. A recent publication aims to define the association between circulating tumor DNA level and disease stage in patients with newly diagnosed endometrial cancer and determine whether sequencing of longitudinal cell-free DNA samples can be used for disease monitoring and detection of progression or recurrence. These results accelerate the current knowledge of molecular follow-up in endometrial cancer.
See related article by Ashley et al., p. 410
Dysregulated Lipid Synthesis by Oncogenic IDH1 Mutation Is a Targetable Synthetic Lethal Vulnerability
Authors: Thomas Daniel , Wu Manhong , Nakauchi Yusuke , Zheng Ming , Thompson-Peach Chloe A.L. , Lim Kelly , Landberg Niklas , Köhnke Thomas , Robinson Nirmal , Kaur Satinder , Kutyna Monika , Stafford Melissa , Hiwase Devendra , Reinisch Andreas , Peltz Gary , Majeti Ravindra 
Published: November 2022

AbstractIsocitrate dehydrogenase 1 and 2 (IDH) are mutated in multiple cancers and drive production of (R)-2-hydroxyglutarate (2HG). We identified a lipid synthesis enzyme [acetyl CoA carboxylase 1 (ACC1)] as a synthetic lethal target in mutant IDH1 (mIDH1), but not mIDH2, cancers. Here, we analyzed the metabolome of primary acute myeloid leukemia (AML) blasts and identified an mIDH1-specific reduction in fatty acids. mIDH1 also induced a switch to b-oxidation indicating reprogramming of metabolism toward a reliance on fatty acids. Compared with mIDH2, mIDH1 AML displayed depletion of NADPH with defective reductive carboxylation that was not rescued by the mIDH1-specific inhibitor ivosidenib. In xenograft models, a lipid-free diet markedly slowed the growth of mIDH1 AML, but not healthy CD34+ hematopoietic stem/progenitor cells or mIDH2 AML. Genetic and pharmacologic targeting of ACC1 resulted in the growth inhibition of mIDH1 cancers not reversible by ivosidenib. Critically, the pharmacologic targeting of ACC1 improved the sensitivity of mIDH1 AML to venetoclax.Significance:Oncogenic mutations in both IDH1 and IDH2 produce 2-hydroxyglutarate and are generally considered equivalent in terms of pathogenesis and targeting. Using comprehensive metabolomic analysis, we demonstrate unexpected metabolic differences in fatty acid metabolism between mutant IDH1 and IDH2 in patient samples with targetable metabolic interventions.See related commentary by Robinson and Levine, p. 266.This article is highlighted in the In This Issue feature, p. 247
Legenda profissional e amadora: um estudo descritivo-contrastivo baseado na série espanhola Gran Hotel
Authors: Malta Gleiton , Souza Priscyla Gomes de 
Published: November 2022

Inserido no campo disciplinar dos Estudos da Tradução, este estudo tem como objetivo principal contrastar um corpus composto por legendas em português do Brasil extraído da série espanhola Gran Hotel. Especificamente, objetiva-se (i) verificar se as legendas seguem as normas vigentes de legendagem; registrar diferenças e (ii) identificar sua natureza, se relacionadas à norma ou à estrutura linguístico-semântica. Por último, (iii) propor soluções para alguns problemas de tradução identificados. Como resultados, averiguou-se que a legenda profissional demonstra maior cuidado no que tange às normas de legendagem e segmentação textual em comparação com a legenda amadora. Esta, por sua vez, foge dos padrões estabelecidos como corretos. Espera-se que este estudo contribua para novas pesquisas no âmbito da tradução para legendagem, campo fértil a ser explorado academicamente no nicho da tradução audiovisual.
A High Cholesterol Diet During Late Pregnancy Impairs Long-Term Maternal Vascular Function in Mice
Authors: Sáez Tamara , Pageé Abbey , Kirschenman Raven , Quon Anita , Spaans Floor , Davidge Sandra T. 
Published: November 2022

Background:
Gestational dyslipidemia is associated with pregnancy complications including preeclampsia. However, whether gestational dyslipidemia leads postpartum vascular dysfunction, which could increase the risk for cardiovascular complications later in life, is not known. Here, we aimed to determine whether a gestational dyslipidemia affects postpartum vascular health and induces early signs of atherosclerosis.

Methods:
Pregnant C57BL/6 mice received a high cholesterol diet or control diet from gestational day 13.5 until term. After delivery, all mice received the control diet for ≈3 months postpartum (PP). Age-matched nulliparous females were on the same diets for equal periods. After 3 months, all mice were euthanized, serum was collected, and aortas were isolated to assess vascular function (wire myography) and markers of oxidative stress and early atherosclerosis.

Results:
PP-high cholesterol diet females had increased circulating cholesterol levels compared with PP-control diet mice, without effect of the diet in nulliparous mice. Methacholine-induced vasodilation was impaired, and nitric oxide contribution reduced, by the high cholesterol diet in aortas of PP mice, but not in nulliparous mice. Exposure to oxidized low-density–protein cholesterol further impaired methylcholine-induced vasodilation in PP-high cholesterol diet aortas only. Compared with PP-control diet mice, aortic inducible nitric oxide synthase expression, reactive oxygen species and nitrotyrosine levels were increased in aortas from PP-high cholesterol diet mice. No differences in aortic lipid deposition and macrophage infiltration were found.

Conclusions:
Exposure to a high cholesterol diet in pregnancy impairs vascular function postpartum. Our results support the hypothesis that gestational dyslipidemia impacts maternal vascular function after pregnancy, which could potentially predispose these women to future cardiovascular complications.