bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lymphatic metastasis-associated circRNA‒miRNA‒mRNA network for exploring the pathogenesis and therapeutic target of triple negative breast cancer based on whole-transcriptome sequencing analysis: an experimental verification study
Authors: Luo Jiayue , Cao Dong , Hu Chuwen , Liang Zhen , Zhang Yuanping , Lai Jianguo 
Published: November 2022

Abstract
Background
The metastatic mechanisms of axillary lymph nodes (ALNs) in triple-negative breast cancer (TNBC) remain unclear. We aimed to identify the potential circRNA regulatory network in ALN metastasis.

Methods
We performed whole transcriptome sequencing (WTS) to determine the expression profiles of RNAs and screen out differentially expressed messenger RNAs (DEMs), microRNAs (DEMis), and circRNAs (DECs) between ALN-positive and ALN-negative TNBC patients. Functional enrichment analysis and Kaplan–Meier survival analysis were utilized to unearth the potential regulatory mechanisms of the DEMs. A competing endogenous RNA (ceRNA) network was constructed using computational biology. The expression levels of DECs in cell lines were confirmed by real-time polymerase chain reaction (RT‒PCR).

Results
Following WTS and differential expression analysis, 739 DEMs, 110 DEMis, and 206 DECs were identified between ALN-positive and ALN-negative TNBC patients. Functional analysis indicated that the DEMs mainly functioned in carcinogenesis and tumor progression-related pathways. ceRNA networks containing eight circRNAs, six miRNAs, and eighteen mRNAs were developed. In the ceRNA network, two mRNAs (RAB3D and EDARADD) that were significantly associated with better overall survival and one mRNA (GSR) that predicted favorable recurrence-free survival in TNBC patients were chosen for further analysis. Then, a survival-related ceRNA network containing two DECs (hsa_circ_0061260 and hsa_circ_0060876), two DEMis (hsa-miR-5000-3p and hsa-miR-4792), and three mRNAs (GSR, RAB3D, and EDARADD) was identified. Then, two candidate DECs were validated by real-time PCR.

Conclusion
Our research constructed a ceRNA network that provides novel insights into the molecular mechanism of ALN metastasis and potential therapeutic targets in TNBC.
Assessment of selected heavy metals in honey samples using flame atomic absorption spectroscopy (FAAS), Ethiopia
Authors: Tibebe Dessie , Hussen Mohammed , Mulugeta Marye , yenealem Dereje , Moges Zerubabile , Gedefaw Mohammed , Kassa Yezbie 
Published: November 2022

AbstractHoney is a natural, sugary and sticky liquid that is produced from the nectars of flowers by the bees. This study aimed to analyze the concentration of some selected heavy Metals in honey samples. 1 g of honey sample was digested by a hot plate using 9ml of HNO3 and 3ml of H2O2. The concentrations of the heavy metals in the digested were detected using a flame atomic absorption spectrometer. The results of this study found that the concentrations of the heavy metals in the honey samples were ranged from 1.97 to 2.04 µg/g for Zn, 1.93 µg/g to 2 µg/g for Cu, 0.83 to 1.01 µg/g for Mn, 0.25 to 0.45 µg/g for Cr, 0.025–0.031 µg/g for Cd. However, Pb was not detected in all honey samples. Hence, the levels of heavy metals found were below the permitted levels set by the World Health Organization. From the results, the levels of heavy metals found were below the permitted levels set by the World Health Organization. Thus, the heavy metals in the sampled honey are safe for human consumption in these selected areas.
Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods
Authors: Gow Ineka , Smith Nicholas C. , Stark Damien , Ellis John 
Published: November 2022

AbstractLeishmaniainfections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world’s poorest regions. Transmitted by the bite of a female sand fly,Leishmaniainfections are increasing with human movement (due to international travel and war) as well as with shifts in vector habitat (due to climate change). Accurate diagnosis of the 20 or so species ofLeishmaniathat infect humans can lead to the successful treatment of infections and, importantly, their prevention through modelling and intervention programs. A multitude of laboratory techniques for the detection ofLeishmaniahave been developed over the past few decades, and although many have drawbacks, several of them show promise, particularly molecular methods like polymerase chain reaction. This review provides an overview of the methods available to diagnostic laboratories, from traditional techniques to the now-preferred molecular techniques, with an emphasis on polymerase chain reaction-based detection and typing methods.Graphical abstract
Partial otubain 1 deficiency compromises fetal well-being in allogeneic pregnancies despite no major changes in the dendritic cell and T cell compartment
Authors: Stutz Annika , Nishanth Gopala , Zenclussen Ana C. , Schumacher Anne 
Published: November 2022

Abstract
Objective
Pregnancy is characterized by well-defined immunological adaptions within the maternal immune cell compartment allowing the survival of a genetically disparate individual in the maternal womb. Phenotype and function of immune cells are largely determined by intracellular processing of external stimuli. Ubiquitinating and deubiquitinating enzymes are known to critically regulate immune signaling either by modulating the stability or the interaction of the signaling molecules. Accordingly, if absent, critical physiological processes may be perturbed such as fetal tolerance induction. Based on previous findings that mice hemizygous for the deubiquitinating enzyme otubain 1 (OTUB1) do not give rise to homozygous progeny, here, we investigated whether partial OTUB1 deficiency influences fetal-wellbeing in a syngeneic or an allogeneic pregnancy context accompanied by changes in the dendritic cell (DC) and T cell compartment.

Results
We observed increased fetal rejection rates in allogeneic pregnant OTUB1 heterozygous dams but not syngeneic pregnant OTUB1 heterozygous dams when compared to OTUB1 wildtype dams. Fetal demise in allogeneic pregnancies was not associated with major changes in maternal peripheral and local DC and T cell frequencies. Thus, our results suggest that OTUB1 confers fetal protection, however, this phenotype is independent of immune responses involving DC and T cells.
Secure routing protocol based on dynamic reputation and load balancing in wireless mesh networks
Authors: Yuan Xiaogang , Chen Yipiao 
Published: November 2022

AbstractAiming at the characteristics of dynamic topology change and vulnerability of wireless mesh networks (WMN), a secure routing protocol based on dynamic reputation and load balance (SRP-DRLB) is proposed to improve the routing security and load balance. By dynamically predicting of the reputation and traffic of the node in WMN, SRP-DRLB adopts edge weight strategy and node load idleness to avoid the competitive access of a large number of data packets to nodes with high reputation. SRP-DRLB dynamically adjusts routes according to the real-time status of network nodes’ reputation and traffic, reducing the impact of malicious nodes on the network and the overload of high-reputation nodes, thus improving the identification rate of malicious nodes and node load balancing. Simulation results show that SRP-DRLB can effectively ensure the security of data transmission, and it has the advantages of improving the identification rate of malicious nodes, the success rate of packet transmission and avoiding network congestion.
The EU and the Western Balkans’ response during the migrant crisis
Authors: Komljenović Marija 
Published: November 2022

AbstractBackgroundThe European Green Deal does not consider the issue of migrant inclusion, nor are there specific data on the risk of migrants falling into energy poverty, social exclusion, and other data that could help create an effective policy that would be applied in this area. The main objective of this study is to assess the current status of the migrant population when it comes to risk from poverty (including energy poverty) and social exclusion in four (4) EU countries with the most significant influx of migrants.ResultsAn analysis included two main indicators aimed to describe level of risk of migrants to fall into energy poverty: (a) persons at risk of poverty or social exclusion by group of citizenship (population aged 18 and over, male and female); and (b) Migrant Integration Policy Index. The results revealed that foreign-born citizens have greater poverty or social exclusion risk. In 2020, citizens of Sweden that were not born in the EU28 had more than 360% greater chance than national citizens of being at risk of poverty (including energy poverty) or social exclusion. In France, foreign citizens were 340% more likely to be exposed to poverty than national citizens, while Germany records the highest rate: 250% in 2020. The United Kingdom rates demonstrate an increased 10% (2014–2018) difference in the risk of poverty (including energy poverty) or social exclusion for foreign citizens. In addition, results obtained by using the Migrant Integration Policy Index showed highest level of Anti-discrimination score is highest in Sweden, and lowest in Albania. On the other hand, Labor market mobility score is highest in Sweden, and lowest in Slovenia.ConclusionsBearing in mind the results of the research, the primary recommendations elaborated in the paper are as follows: (a) up-to-date collection and innovation of data for the migrant population (including data on energy poverty); (b) creation of conditions for more efficient employment of migrants; (c) adoption of special measures to protect migrants from energy poverty, and (d) strengthening of cooperation between the EU and to non-EU countries on the migrant route.
Global trends and frontiers in research on coronary microvascular dysfunction: a bibliometric analysis from 2002 to 2022
Authors: Gao Jing , Meng Tiantian , Li Min , Du Ruolin , Ding Jingyi , Li Anqi , Yu Shanshan , Li Yixiang , He Qingyong 
Published: November 2022

Abstract
Background
Coronary microvascular dysfunction (CMD) is a leading cause of ischemic heart disease. Over the past few decades, considerable progress has been made with respect to research on CMD. The present study summarized the current research hotspots and trends on CMD by applying a bibliometric approach.

Methods
Relevant publications between 2002 and 2022 were extracted from the Web of Science Core Collection. Visualization network maps of countries, institutions, authors, and co-cited authors were built using VOSviewer. CiteSpace was used for keyword analysis and the construction of a dual-map overlay of journals and a timeline view of co-cited references.

Results
1539 CMD-related publications were extracted for bibliometric analysis. The annual publications generally showed an upward trend. The United States of America was the most prolific country, with 515 publications (33.5%). Camici P. G. was the most influential author, whereas the European Heart Journal, Circulation, and Journal of the American College of Cardiology were the most authoritative journals. Research hotspot analysis revealed that endothelial dysfunction as well as reduced nitric oxide production or bioavailability played critical roles in CMD development. Positron emission tomography was the most widely used imaging method for diagnosis. In addition, microvascular angina, hypertrophic cardiomyopathy, and heart failure have attracted much attention as the main clinical implications. Furthermore, international standards for CMD diagnosis and management may be the future research directions.

Conclusions
This study offers a comprehensive view about the hotspots and development trends of CMD, which can assist subsequent researchers and guide future directions.
Chinese patients’ response to doctor–patient relationship stimuli: evidence from an event-related potential study
Authors: Ye Zehan , Pang Jiaoyan , Ding Wei , He Wen 
Published: November 2022

Abstract
Background
With improvements in medical technology, the doctor–patient relationship should be further improved. However, disputes between doctors and patients have increased, with the two groups frequently hurting each other. Therefore, we sought to explore the perception of Chinese patients regarding the stimuli of doctor–patient relationships with different valence.

Methods
We used event-related potential (ERP) to explore the brain electrical activity of 19 undergraduate participants who had a clinical experience in the previous 6 months where they perceived negative, neutral, and positive doctor–patient relationships. The ERPs were recorded, and the early ERP components (P2) and late positive potential (LPP) were measured.

Results
Compared with the stimuli of negative doctor–patient relationships, those of positive doctor–patient relationships would attract more attention and have larger P2 amplitude; LPP was larger for the stimuli of negative doctor–patient relationships than neutral ones in the 500–800 ms, while in the 1100–1500 ms, the stimuli of neutral doctor–patient relationships elicited larger LPP amplitude than positive ones.

Conclusion
Patients paid more attention to the stimuli of positive doctor–patient relationships because they expected to have the same positive relationship. Although threatening elements in negative doctor–patient relationships would catch patients’ attention and make them have implicit emotional regulation, neutral stimuli with poker-faced doctors would cause lasting attention. These results illustrate the patients’ real perception of the different valence of doctor–patient relationship stimuli.
The assessment, development, and measurement of human capacity building programs for El Salvador’s textile and apparel industry
Authors: McAndrews Laura E. , Ha-Brookshire Jung E. 
Published: November 2022

AbstractSustained competitive advantage in developing nations is driven by human development through intangible assets that come from education. Grounded from Barney’s (Journal of Management 17:99–120, 1991) resource-based theory, this study was designed (a) to assess El Salvador’s textile and apparel (T&A) industry by identifying key knowledge gaps, (b) to create human capacity building opportunities using Kolb’s experiential learning theory for T&A supply chain members, and (c) to measure effectiveness of such opportunities through principle-attributes matrix, ultimately helping the industry sector compete. A case study research study approach was used to develop a comprehensive approach to develop four educational workshops for Salvadorian T&A supply chain members from April 2019 to February 2020. Pre and post surveys measured workshops’ effectiveness in knowledge, skills, and attitudes. The results showed that there was a statistically significant difference in pre- and post means for knowledge, skills, and attitudes for all four workshops. Further analysis showed that each of the workshops statistically changed participants’ human capacity in supply chain management, product development, marketing, and CAFTA-DR provisions. The study shows that educational theory could be used successfully for human capacity building in developing nations and supply chain training.
Does Hypoxia and Stress Erythropoiesis Compromise Cardiac Function in Healthy Adults? A Randomized Trial
Authors: Arrebola-Moreno Antonio L. , Casuso Rafael A. , Bejder Jacob , Bonne Thomas Christian , Breenfeldt Andersen Andreas , Aragón-Vela Jerónimo , Nordsborg Nikolai B. , Huertas Jesús R. 
Published: November 2022

Abstract
Objectives
To investigate whether recombinant human erythropoietin (rHuEPO) injections during an altitude training camp impact heart function.

Methods
Thirty (12 women) moderately trained subjects stayed at 2320 m altitude for 4 weeks while training. Subjects were randomized to placebo (isotonic saline) or rHuEPO (20 IU/kg body weight) i.v. injections. Transthoracic echocardiography imaging was acquired 3 days after arrival to altitude and prior to the first placebo or rHuEPO injection as well as one day after the last rHuEPO injection three weeks later.

Results
rHuEPO did not alter cardiovascular morphology parameters, systolic or diastolic function. In the placebo group, altitude exposure improved left ventricle (LV) systolic function due to an increased twist angle but rHuEPO had no additional effects. Pulmonary arterial systolic pressure was unaffected in either group. Notably, rHuEPO hampered LV untwist rate without affecting LV early filling.

Conclusion
rHuEPO provided during mild altitude exposure does not cause any major effects on heart function. The observed alteration in LV untwist induced by rHuEPO is unlikely to have a meaningful clinical effect.
Trial Registration Registered on www.clinicaltrials.gov (NCT04227665).
Robot-assisted early mobilization of intensive care patients: a feasibility study protocol
Authors: Warmbein Angelika , Schroeder Ines , Mehler-Klamt Amrei , Rathgeber Ivanka , Huber Jana , Scharf Christina , Hübner Lucas , Gutmann Marcus , Biebl Johanna , Lorenz Andreas , Kraft Eduard , Zoller Michael , Eberl Inge , Fischer Uli 
Published: November 2022

Abstract
Background
Early mobilization positively influences the outcome of critically ill patients, yet in clinical practice, the implementation is sometimes challenging. In this study, an adaptive robotic assistance system will be used for early mobilization in intensive care units. The study aims to evaluate the experience of the mobilizing professionals and the general feasibility of implementing robotic assistance for mobilization in intensive care as well as the effects on patient outcomes as a secondary outcome.

Methods
The study is single-centric, prospective, and interventional and follows a longitudinal study design. To evaluate the feasibility of robotic-assisted early mobilization, the number of patients included, the number of performed VEM (very early mobilization) sessions, and the number and type of adverse events will be collected. The behavior and experience of mobilizing professionals will be evaluated using standardized observations (n > 90) and episodic interviews (n > 36) before implementation, shortly after, and in routine. Patient outcomes such as duration of mechanical ventilation, loss of muscle mass, and physical activity will be measured and compared with a historical patient population. Approximately 30 patients will be included.

Discussion
The study will provide information about patient outcomes, feasibility, and the experience of mobilizing professionals. It will show whether robotic systems can increase the early mobilization frequency of critically ill patients. Within ICU structures, early mobilization as therapy could become more of a focus. Effects on the mobilizing professionals such as increased motivation, physical relief, or stress will be evaluated. In addition, this study will focus on whether current structures allow following the recommendation of mobilizing patients twice a day for at least 20 min.

Trial registration
ClinicalTrials.gov, NCT05071248. Date: 2021/10/21
Mixed pulmonary infection with four isolates of nontuberculous mycobacteria: a case report of mycobacterium bacteremicum infection
Authors: Masoumi Morteza , Sakhaee Fatemeh , Zolfaghari Mohammad Reza , Tarashi Samira , Rahimi Jamnani Fatemeh , Vaziri Farzam , Siadat Seyed Davar , Fateh Abolfazl 
Published: November 2022

Abstract
Background
A mixed pulmonary infection of Mycobacterium bacteremicum and three different isolates of nontuberculous mycobacteria (NTM) is an unusual clinical manifestation and have not yet been indicated. In this case report, we reported four isolates of NTM using phenotypic and genotypic test of pulmonary sample in Tehran, Iran.

Case presentation
We report a case of severe pulmonary disease in a 19-year-old male patient with productive cough, shortness of breath, and low-grade fever for several weeks. The C-reactive protein (CRP) level (80.2 mg/L) and erythrocyte sedimentation rate (ESR) (95 mm/h) were high. The computed tomographic scan indicated bronchiectasis, nodular opacities, consolidation, and cavitary lesions on both sides. The result of purified protein derivative (PPD) test was equal to 15 mm. The sequences of hsp65, rpoB, and 16S rDNA genes indicated more than 99% homology to four isolates of nontuberculous mycobacteria (NTM), including Mycobacterium fortuitum, M. chelonae, M. mucogenicum, and M. bacteremicum. We found that all four strains were susceptible to amikacin, cefoxitin, ciprofloxacin, clarithromycin, imipenem, and linezolid. The patient was treated with ciprofloxacin, clarithromycin, and amikacin, along with Montelukast, for five months.

Conclusion
We report a case of severe pulmonary infection by four isolates of NTM. After treatment, the patient reported complete resolution of the signs and a weight gain of 5 kg; also, the CRP and ESR were normal. Nine months after the infection diagnosis, a new CT scan revealed further improvements.
Psychometric evaluation of the Urgency NRS as a new patient-reported outcome measure for patients with ulcerative colitis
Authors: Dubinsky Marla C. , Shan Mingyang , Delbecque Laure , Lissoos Trevor , Hunter Theresa , Harding Gale , Stassek Larissa , Andrae David , Lewis James D. 
Published: November 2022

AbstractBackgroundThe Urgency Numeric Rating Scale (NRS) was developed as a content-valid single-item patient-reported outcome measure to assess severity of bowel urgency. Here, we evaluated the psychometric properties of the Urgency NRS.MethodsData were from a multicenter, randomized, placebo-controlled phase 3 trial in adults with moderately to severely active ulcerative colitis (NCT03518086). Patients completed the Urgency NRS using a daily electronic diary, from which weekly average Urgency NRS scores were calculated. Test–retest reliability, known-groups validity, construct validity, responsiveness, and score interpretation were assessed using the modified Mayo score, Inflammatory Bowel Disease Questionnaire (IBDQ), Patient Global Rating of Severity (PGRS), Patient Global Rating of Change (PGRC), and Geboes score.ResultsThe study sample comprised 1,162 participants (40.2% female). Mean Urgency NRS score was higher (worse) at baseline than at week 12 (6.2 vs. 3.7). Test–retest reliability was strong, with intra-class correlation coefficients of 0.76–0.89. Baseline least-square mean Urgency NRS score was higher for participants with a PGRS score greater than the median (worse symptoms) than for those with a PGRS score less than or equal to the median (7.5 vs. 5.4;p < 0.0001), indicating good known-groups validity. Urgency NRS score was moderately correlated with IBDQ total and domain scores, PGRS, PGRC, and modified Mayo stool frequency, establishing its convergent validity. Correlations were weak for Geboes score and weak to moderate for modified Mayo endoscopic subscore and modified Mayo rectal bleeding, indicating that the Urgency NRS also had discriminant validity. Patients achieving clinical remission, clinical response, IBDQ remission, and PGRS score improvement showed significantly greater improvement on the Urgency NRS (p < 0.0001 for all), demonstrating responsiveness to change. A ≥ 3-point improvement in Urgency NRS score represented a meaningful improvement in bowel urgency and an Urgency NRS score of ≤ 1 point represented a bowel urgency remission threshold that was closely associated with clinical, endoscopic, and histologic remission.ConclusionsThe Urgency NRS is a valid and reliable patient-reported outcome measure that is suitable for evaluating treatment benefits in clinical trials in patients with moderately to severely active ulcerative colitis.