bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tandem Mass Tag-Based Proteomics Analysis Reveals the Mechanism Underlying the Interleukin-6-Mediated Regulation of Trophoblast Function in Preeclampsia
Authors: Han Qing , Zheng Shuisen , Zhang Huale , Yan Jianying 
Published: April 2022

Abstract
Objective we investigate the mechanism whereby interleukin-6 (IL-6), an important inflammatory marker, influences trophoblast function during preeclampsia. Methods Quantitative PCR and enzyme-linked immunosorbent assay were used to determine the IL-6 mRNA and protein levels, respectively. CCK8 and transwell assays were used to detect how IL-6 affects the proliferation and invasion abilities, respectively, of HTR-8/SVneo cells; the tube-forming assay was used to detect how IL-6 affects the angiogenesis ability of human umbilical vein endothelial cells (HUVECs) after their co-culture with HTR-8/SVneo cells. Using tandem mass tag-based proteomics analysis, we screened for different proteins before and after IL-6 stimulation; Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to explore the functions and signal pathways associated with, these proteins. Results The IL-6 levels were higher in the placenta of preeclampsia group than in normal group. IL-6 suppressed the proliferation and invasion of HTR-8/SVneo cells, but promoted the angiogenesis of HUVECs. Seventy differentially expressed IL-6 downstream proteins were identified; these were enriched with various biological processes, molecular functions, cellular components, and biological pathways. Conclusions IL-6 regulates trophoblast function by interacting with multiple proteins and pathways. Proteomics-based screening represents a macroscopic means for elucidating the molecular mechanisms underlying preeclampsia.
Demography of ethnic minorities and its effects on the genetic diversity of rice landraces in Guizhou Province, China
Authors: Liu Chunhui , Wang Yanjie , Jiao Aixia , Ma Xiaoding , Cui Di , Li Xiaobing , Han Bing , Chen Huicha , Ruan Renchao , Xue Dayuan , Han Longzhi 
Published: April 2022

Abstract
Ethnic minorities living in Guizhou Province have a long history of rice farming and traditional culture, and they produce numerous of rice landraces, which are rich in genetic variation. Therefore, studying the genetic structure and diversity of rice landraces in Guizhou has become a hot topic. However, the influence of ethnic minorities and their traditional farming system and culture on rice landraces remains unclear. In this study, we analyzed the genetic diversity and population structure in 598 rice landraces in six eco-rice farming zones using simple sequence repeat (SSR) markers and the nucleotide variation between two similar populations collected during different periods using single nucleotide polymorphism (SNP) haplotype analysis of six unlinked nuclear loci. The results were as follows: 1) The genetic diversity level of rice landraces in six different eco-rice farming zones of Guizhou Province was high (He = 0.7659), and the genetic background was rich. Qianxinan Miao and Buyi Autonomous Prefecture is the genetic diversity center of rice landraces in Guizhou, with the highest He (0.7549) and PIC (0.7278). 2) The neighbor-joining (N-J) tree and the STRUCTURE model showed the same genetic structure, and the population structure of japonica was correlated with geographical origin. Under the influence of the domestication of the Buyi, Miao and Dong people, rice landraces in the III and IV eco-rice farming zones were isolated into a subgroup, forming a special ecological type of landrace. 3) Nucleotide variation analysis was performed on similar rice landraces collected in 1980 and 2015. The results showed that after 35 years of domestication, the original dominant haplotypes could be preserved well, and the frequency of the most favorable haplotypes gradually increased, while the frequency of a few unfavorable haplotypes gradually decreased to adapt to the corresponding environment and traditional culture. This study can promote the protection and sustainable utilization of rice landraces and provide more valuable germplasm materials and information for rice breeding and basic research.
Th2 cells are associated with tumor recurrence following radiation.
Authors: Abdelhakiem Mohamed , Bao Riyue , Pifer Phillip M. , Molkentine David , Molkentine Jessica , Hefner Andrew , Beadle Beth M. , Heymach John , Luke Jason J. , Ferris Robert L. , Pickering Curtis , Wang Jing H. , Patel Ravi B. , Skinner Heath D. 
Published: April 2022

Abstract

Background
Curative treatment of the most aggressive solid tumors utilizes radiation therapy, either as a monotherapy or combined with surgery and/or chemotherapy. Previously, we linked the expression of PD-L1 with clinical radioresistance; however, the relationship between outcome following radiation and immune function is unclear.
Methods
We used two well-annotated cohorts to examine clinical outcomes. The discovery cohort included 94 patients diagnosed with Head and Neck squamous cell carcinoma (HNSCC) who were treated uniformly with surgery and adjuvant radiation. The validation cohort consisted of 97 patients with similar treatment. Immune infiltrates in tumors were derived from RNAseq gene expression in tumor tissues using xCell. The association between each immune cell type and clinical outcomes was tested using Cox proportional hazards models. Immune cell types significantly associated with overall survival (OS), distant metastasis (DM), or locoregional recurrence (LRR) in the discovery cohort were examined in the independent validation cohort. Genes that carry nonsynonymous somatic mutations or have mRNA expression that is associated with the abundance of CD4 + T helper 2 (Th2) cell infiltrate in tumors were also identified in the full TCGA HNSCC cohort as well as non-overlapping cohorts from the International Cancer Genome Consortium (ICGC) and the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC).
Results
In the discovery cohort, we identified intratumoral cell types significantly associated with OS (monocytes, CD4 + memory T cells & Th2 cells), DM (CD4 + memory T cells, sebocytes, and epithelial cells) or LRR (common lymphoid progenitor (CLP) cells, CD4 + memory T cells, Th2 cells, and immature dendritic cells (iDCs). However, only a positive association between high levels of Th2 cells and LRR was identified in the validation cohort. We also identified CREBBP/EP300 and CASP8 among the genes that carry somatic mutations significantly associated with the presence of Th2 cells. Additionally, pathway analysis of genes correlated with Th2 cells revealed potential repression of the antitumor immune response and activation of BRCA1-associated DNA damage repair in multiple cohorts.
Conclusions
Th2 infiltrate is enriched in HPV-negative HNSCC tumors and is associated with LRR in two independent cohorts of patients following surgery and radiation. Th2 infiltrate is associated with mutations in CASP8 and CREBBP/EP300 and pathways previously shown to impact the response to radiation. Further investigation is warranted to investigate the mechanisms underlying the role of Th2 cells in mediating radioresistance in HPV-negative HNSCC.
Transmissibility and control of tuberculosis: a modeling study based on four outbreaks in China
Authors: Liu Chan , Yang Meng , Chen Qiuping , Jia Jing , Zhao Zeyu , Xu Jingwen , Zhao Benhua , Su Yanhua , Gavotte Laurent , Frutos Roger , Chen Tianmu 
Published: April 2022

Abstract
Background: As the second largest burden country, China displays a high incidence of tuberculosis with frequent occurrence of outbreaks. Summarizing and analyzing the characteristics of tuberculosis outbreaks can help to quantify the transmissibility and to evaluate the effectiveness of intervention measures, providing thus technical support for prevention and control. Methods: We collected data on 4 tuberculosis outbreaks in Changsha city, Hunan Province. Susceptible-Exposed-Infectious-Recovered (SEIR) model was used to fit collected data and calculate transmissibility. Susceptible-Exposed-Infectious-Quarantined-Recovered (SEIQR) model was used to evaluate the effect of isolation. Effective reproduction number and prevalence rate were used to quantify the transmissibility of tuberculosis. Results: The number of male patients was higher than that of female patients in 4 outbreaks. Most student cases were distributed in high grades of high school and universities. The Reff of the 4 outbreaks were 15.22, 12.87, 13.47 and 2.70, respectively. Isolating 100% of the patients at first month could reduce the number of cases by 96.45%, 96.36%, 97.08% and 100.00%, respectively. The median Reff of 4 outbreaks in Changsha city was 13.17 (IQR 10.33-13.91). Conclusions: Early detection and isolation of cases are effective methods of prevention of tuberculosis outbreaks.
Sequential tocilizumab and tofacitinib treatment for systemic juvenile idiopathic arthritis: a case report
Authors: Zhang Ye , Ru Jinli , Zhang Jinxiu 
Published: April 2022

Abstract
Background: Systemic juvenile idiopathic arthritis (sJIA) is complex, difficult to cure, and has high morbidity and mortality rates. Herein, we report a case of a patient with sJIA who was treated with sequential tocilizumab and tofacitinib treatment.Case presentation: The patient was a 4-year-old girl who was hospitalised with recurrent fever and polyarticular pain in June 2020. Laboratory tests revealed a white blood cell count of 15.32 × 109/L, platelet count of 676.8 × 109/L, serum ferritin level of 1,103.8 U/L, erythrocyte sedimentation rate of 85.00 mm/h, c-reactive protein level of 146.00 g/L, and interleukin (IL)-6 level of 287.95 pg/ml. Rheumatoid factor and antinuclear antibody results were negative, and she was diagnosed with sJIA. The patient was started on a combination of ibuprofen, methotrexate, and tocilizumab, and her temperature decreased to the normal range without any recurrence. The painful joint swellings resolved significantly after 3 months of follow-up. Janus kinase (JAK) inhibitors inhibit the effects of many cytokines, particularly IL-6, and is economical and convenient. Therefore, we selected tofacitinib to replace tocilizumab for her treatment, whereas the other drugs remained unchanged. Arthritis symptoms disappeared gradually after 9 months of follow-up. In May 2021, due to a slight recurrence of upper respiratory tract infection, the patient was hospitalised, administered one dose of tocilizumab, and continued to take oral tofacitinib, which quickly relieved the patient's symptoms. By December 2021, the patient's condition was stable.Conclusions: The efficacy of treatment with sequential tofacitinib via tocilizumab in our patient with sJIA was found to be remarkable. IL-6 inhibitors sequential to JAK inhibitors could be a new option for the treatment of systemic juvenile idiopathic joints.
Pulmonary embolism as a cause of suicide attempt by stabbing in the chest. Clinical dilemmas
Authors: Nożewski Jakub , Nowak Ewa , Dura Marta , Grześk Grzegorz , Kłopocka Maria 
Published: April 2022

Abstract
BackgroundPulmonary embolism is the third most common cause of cardiovascular death. While the conditions that rank 1st and 2nd manifest with characteristic symptoms, pulmonary embolism is a tricky, often oligosymptomatic disease. While in the case of stable patients, diagnosed with a low-risk disease, the treatment is predictable, in patients with co-existing medical conditions, anticoagulation treatment leads to serious bleeding in 7.22 out of 100 treated patients/year, with a mortality rate reaching 9%.Case presentationThis report presents a case of a male patient with self-inflicted knife chest wound as a result of severe chest pain due to massive pulmonary embolism, as well as the resulting clinical dilemmas.ConclusionsThe above case is a rarely seen concomitance. It describes an invariably rare attempt to deal with the untreatable pain of a pulmonary embolism. I will certainly be a guide for other doctors that it is possible to perform similar procedures without endangering the patient's life despite clear contraindication in guidelines.
Estimates of genomic heritability and genome-wide association studies for blood parameters in Akkaraman sheep
Authors: Arzik Yunus , Kizilaslan Mehmet , White Stephen N. , Piel Lindsay M.W. , Cinar Mehmet Ulas 
Published: April 2022

Abstract
The aim of this study was to estimate genomic heritability and the impact that genetic backgrounds have on blood parameters in Akkaraman sheep by conducting genome-wide association studies. Genomic heritability estimates for blood parameters ranged from 0.00 to 0.55, indicating that measured phenotypes have a low to moderate heritability. A total of 7 genome- and 13 chromosome-wide significant SNPs were associated with phenotypic changes in 15 blood parameters tested. Accordingly, SCN7A, SCN9A, MYADM-like, CCDC67, ITGA9, MGAT5, SLC19A1, AMPH, NTRK2, MSRA, SLC35F3, HDAC4, SIRT6, CREB3L3, OSBPL8, ZDHHC17, CSRP2, NAV3 and E2F7 genes as well as three undefined regions (LOC101117887, LOC106991526 and LOC105608461) were suggested as candidates. Most of the identified genes were involved in basic biological processes that are essential to immune system function and cellular growth; specific functions include cellular transport, histone deacetylation, cell differentiation, erythropoiesis, and endocytosis. The top significant SNP for HCT, MCH, and MCHC was found within a genomic region mainly populated by the MYADM-like gene family. This region was previously suggested to be under historical selection pressure in many sheep breeds from various parts of the world. These results have implications on animal breeding program studies due to the effect that the genetic background has on blood parameters, which underlying many productive and wellness related traits.
Development and validation of clinical risk score model for predicting progression of COVID-19 among Iranian patients
Authors: Mohammadzadeh Fatemeh , Noghabi Ali Delshad , Bilondi Sina Sabeti , Tavakolizadeh Mitra , Hajavi Jafar , Aalami Hosein , Sahebanmaleki Mohsen 
Published: April 2022

Abstract
There are few scoring systems within the clinical setting for early figuring out high-risk COVID-19 patients for severe illness and negative consequences. The present study aimed to create a risk prediction score that could identify patients whose disease is probably to progress at the time of hospitalization. From April to June 2021, 171 confirmed COVID-19 patients admitted to Bohlool hospital, Iran, were consecutively enrolled in this prospective study. First, the risk score prediction model was constructed the use of a logistic regression model. Then, the validation of the risk score was assessed in an independent sample of patients. 141 (82.5%) were recovered or showed symptomatic improvement. Nine patients (5.2%) had progressed to severe disease, while 21 patients (12.3%) died. Three independently significant variables, including IL-6, NLR, and lung involvement were included in the risk score. The discrimination ability of the risk score was good, with the area under the receiver operating characteristic curve (ROC) 0.970 (95% CI: 0.935, 1.00) in the discovery dataset and 0.973 (95% CI: 0.923, 1.00) in the validation dataset. We discovered a risk score that might be utilized as a clinical diagnostic tool to identify COVID-19 individuals who are at increased risk of illness progression.
What Have We Learned from OpenReview?
Authors: Wang Gang , Peng Qi , Zhang Yanfeng , Zhang Mingyang 
Published: April 2022

Abstract
Anonymous peer review is used by the great majority of computer science conferences. OpenReview is such a platform that aims to promote openness in peer review process. The paper, (meta) reviews, rebuttals, and final decisions are all released to public. We collect 11,915 submissions and their 41,276 reviews from the OpenReview platform. We also collect these submissions' citation data from Google Scholar and their non-peer-reviewed versions from arXiv.org. By acquiring deep insights into these data, we have several interesting findings that could help understand the effectiveness of the public-accessible double-blind peer review process. Our results can potentially help writing a paper, reviewing it, and deciding on its acceptance.
Investigation of the Relationship of CO-RADS and CT Patterns with Laboratory Parameters in COVID-19 Patients and a New Perspective on the Total CT Scoring System
Authors: Aydin Nevin , Yildiz Pinar , Cansu Döndü Üsküdar , Gündogdu Elif , Mutluay Rüya , Yorulmaz Göknur , Tekin Melisa Sahin , Kocaturk Evin , Alatas İbrahim Özkan , Kartal Elif Doyuk , Erben Nurettin , Durmaz Gül , Kasifoglu Nilgun , Us Tercan , Sahin Garip , Bal Cengiz , Yilmaz Senay , Korkmaz Cengiz 
Published: April 2022

Abstract
Background: It is important to determine the correlation of the CO-RADS classification and computed tomography (CT) patterns of the lung with laboratory data. To investigate the relationship of CO-RADS categories and CT patterns with laboratory data in patients with a positive RT-PCR test. We also developed a structured total CT scoring system and investigated its correlation with the total CT scoring system.Method: The CT examinations of the patients were evaluated in terms of the CO-RADS classification, pattern groups and total CT score. Structured total CT score values were obtained by including the total CT score values and pattern values in a regression analysis. The CT data were compared according to the laboratory data. Results: A total of 198 patients were evaluated. There were significant differences between the CO-RADS groups in terms of age, ICU transfer, oxygen saturation, creatinine, LDH, D-dimer, high-sensitivity cardiac troponin-T (hs-TnT), CRP, structured total CT score values, and total CT score values. A significant difference was also observed between the CT pattern groups and oxygen saturation, creatinine and CRP values. When the structured total CT score values and total CT score values were compared they were observed to be correlated.Conclusions: Creatinine can be considered as an important marker for the CO-RADS and pattern classifications in lung involvement. LDH can be considered as an important marker of parenchymal involvement, especially bilateral and diffuse involvement. The structured total CT scoring system is a new system that can be used as an alternative.
Selective Outcome Reporting Bias Is Highly Prevalent in Randomized Clinical Trials of Periodontology
Authors: Souza Nathalia Vilela , Nicolini Alessandra Cardoso , Reis Isabella Neme Ribeiro , Sendyk Daniel Isaac , Cavagni Juliano , Pannuti Claudio Mendes 
Published: April 2022

Abstract
Objectives: This study aimed to investigate the prevalence of a type of bias named Selective Outcome Reporting (SOR) in publications of RCTs on non-surgical periodontal therapy (NSPT) and verify its associated factors. Materials and Methods: The protocols were searched and selected on the www.clinicaltrials.gov platform up to January 16, 2022. Corresponding publications were identified, and data extraction and discrepancy analysis were performed. The risk of bias was assessed according to the RoB2 tool. Results: One hundred forty-five studies (174 publications) were included. The prevalence of SOR was 49.7% and was unclear in nearly one third of studies (27.6%). Only 31.7% of the primary outcomes were completely described in the publications. The overall risk of bias was high in 60% of the included studies. SOR was associated with statistical significance (p < .001), and multiple publications of the same study (p = .005). Conclusions: Our study demonstrated the high prevalence of SOR, highlighting the need to improve the quality of reporting of RCTs on NSPT studies. Clinical relevance: SOR is a concerning bias that can affect clinical practice as it increases the effect of the intervention, emphasizing positive results. The incidence of SOR can be reduced by the prospective registry of the study protocol and the complete definition of the outcome. Journal editors could instruct reviewers to check discrepancies between the protocol and the manuscript whenever such information is available.
Incomplete Kawasaki disease with retropharyngeal space abscess as the main manifestation: a case report and literature review
Authors: chen jun , Wen Yang 
Published: April 2022

Abstract
Background: Kawasaki disease (KD) is a childhood acute self-limiting systemic vasculitis of unknown etiology. The diagnosis of KD is based upon clinician's recognition of a symptom pattern. However, manifestations of KD can be atypical, Deep neck infections (DNIs) is a rare manifestation of KD, which can mislead the clinician and delay diagnosis. The following case report describes a patient with incomplete Kawasaki disease (iKD) whose initial presentation a retropharyngeal abscess.Case presentation: A child with clinical manifestations of fever, neck pain, cervical lymphadenopathy, high erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) was reported. The anti-infective treatment was not effective. A retropharyngeal space abscess was detected on neck enhanced Computed Tomography (CT). On the third day following admission, the conjunctival hyperemia, red lips, and strawberry tongue appeared gradually. High-dose of intravenous immunoglobulins (IVIG) and oral aspirin were administered with rapid defervescence, fever and neck pain gradually disappeared, and the neck Magnetic Resonance Imaging (MRI) showed that the abscess was completely resolved.Conclusion: KD with a retropharyngeal space abscess as the first manifestation is rare and may be related to Streptococcus or Staphylococcus aureus infection. When anti-infective treatment was not effective, it is very important to provide timely diagnosis and treatment in combination with other clinical features of KD.
Pediatric patient with Penetrating Brain Injury by a Pickaxe: Case Report and Literature Review
Authors: Mansilla Raíssa , Pinguelo Paulo Henrique Reigota , Pilon Bárbara Contarato , Amaral Luís Gustavo Duarte , Martins Fernando Jorge Monteiro , Silva Geraldo Luiz Paraguassu Correia da 
Published: April 2022

Abstract
Head trauma due to falls is often seen in children, however Penetrating Brain Injury (PBI) – the most life-threatening condition of Traumatic Brain Injury (TBI) - is exceedingly rare. Herein, we report and discuss the challenges encountered in surgical and post-operative management of a 13-old-child patient with PBI by a pickaxe, admitted in Glasgow Coma Scale (GCS) 3 and that not only survived, but also achieved a Glasgow Outcome Scale (GOS) after one year of postoperative follow-up. To our knowledge, this is the first case of pickaxe-induced brain injury on the American Continent and the youngest survivor of this trauma reported in literature.