bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scoping review using PRISMA-SCR on m-health interventions on self-care management of type 2 diabetes at home
Authors: Sinha Abhijeet Prasad , Joshi Ashish , Singhal Manmohan , Gupta Mansi 
Published: June 2022

Abstract
The goal of this study is to conduct a scoping evaluation of the global evidence on m-health interventions and to determine the efficacy of mobile-based self-management interventions for type 2 diabetes mellitus. A rigorous search of the PubMed and Scopus research databases was conducted. Studies on Type 2 diabetes conducted in age ranges 18-75 years and published in English worldwide from 2010 to 2020 are included. Randomized controlled trials (RCTs) using mobile-based self-management for type 2 diabetes patients, as well as recording and analysis of results at the patient level, were among the studies. The studies were checked by the reviewer, and they were then independently reviewed by a second reviewer. The data was extracted, graphed, and the studies' quality was evaluated. To deliver study outcomes and 27 recommendations, the scoping study approach employs Arksey and O'Malley's operational framework. This work is presented using the PRISMA-SCR checklist. The assessment comprised a total of 40 articles, all of which were RCT-based treatments studies with over 3800 participants. For this study, 40 original research publications were chosen. The chosen studies mostly focused on type 2 diabetes self-management (N = 40) and hypertension self-management (N = 2). The usage of mobile SMS (N = 17) or mobile on phone (N = 12), the content of session education with mobile (N = 10) or the placement of mobile devices (N = 4) were among the several treatments. The therapies were usually successful, resulting in better glucose control and improved health indices. Conclusions:For patients with type 2 diabetes, mobile-based self-management interventions appear to have beneficial benefits on self-care and management, as well as meaningful outcomes. Overall improvement in physiologic markers, self-care, and patient quality of life in Type 2 Diabetes patients. Though the research focused on results in small populations, there was little indication that the intervention will be scaled up in the future. Furthermore, the findings suggest that more research into self-management, which includes nutrition informatics in a self-care management regime, is needed, with a particular focus on the availability of nutrition informatics by area. This will also aid in diabetic and cardiovascular disease co-management. In low- and middle-income countries (LMICs), self-management looks to be an effective way of improving health outcomes, hence mobile-led selfcare management treatments incorporating nutritional informatics may be beneficial.
Pulmonary Function Test induction Jacket - A device which reduces the effort related challenges during Forced Vital Capacity maneuver.
Authors: Rajdeep Prashant , Poorey Ketaki , Patel RK , Oommen ER 
Published: June 2022

Abstract
Background- Over the last few years, the prevalence of lung function abnormalities has led to several mass testing programs across the world. PFT induction jacket has been invented as a small step towards making the process of performing FVC maneuver effortless and productive for both the patients and the medical practitioner. Method- The jacket is made up of a three-layered PVC sheet sealed together to form a single jacket with two chambers. The inner chamber is formed between an inner layer and middle layer, in which, when triggered cold water of 10 degree is circulated using connected water unit. Like ways, an outer chamber where air is pressurised using connected air unit is formed between middle layer and outer layer. Thirty volunteers performed FVC maneuver with and without wearing jacket. Results- There was no difference between the results in spirometry parameters ‘without jacket’ and ‘with jacket’. However, Median ± IQR (Q3-Q1) for the number of trials, which a participant had to undergo to perform spirometry was 3 ± 2 (4 − 2) (without jacket) and 1 ± 1 (2 − 1) (with jacket) and this difference was statistically significant (Wilcoxon signed rank test z = 4.129, p < 0.0001), which shows the jacket’s usefulness in performing FVC maneuver. Conclusion- PFT induction jacket has automated the FVC maneuver of computerized Spirometer by triggering physiological inspiratory gasp using cold water and circumscribing pressurised air for expiration. Induction jacket makes the maneuver easy to perform, time efficient and effortless for the patient.
Cadmium speciation in sediments: Effect of grain size, pH, organic acids, and inorganic ions
Authors: Wang Haibo , Zhang Qi , Gomez Mario Alberto , Jia Yongfeng , Yao Shuhua , Li Shifeng 
Published: June 2022

Abstract
In sediments, cadmium (Cd) contamination is an increasingly severe global problem facing environmental protection and human health. The mobility and biological toxicity of Cd are related to its chemical speciation. In this work, the Xihe River in Shenyang, China, was the representative of investigation to study the effect of grain size, pH, organic acids, inorganic anions, and cations on the chemical fractions of Cd in sediments by using the European Community Bureau of Reference (BCR) sequential extraction procedure. The result indicated that fine grains (< 63 μm, 0.57%) presented a relatively higher proportion of the oxidizable fraction Cd in the sediments, while the coarse grains (>250 μm, 78.02%) exhibited the more exchangeable/acid-soluble fraction (78.14 wt.%). The amount of Cd released from the sediment decreased dramatically with the pH from 2-6 and leveled off in the pH range of 8-10. The pH significantly influenced the more mobile fraction (Exchangeable/Acid soluble fraction (F1) and Reducible fraction (F2)), meaning that Cd can be a big-source of danger for the upper water environment. The influence of organic acid on the mobility of Cd in sediments followed the order: ethylenediaminetetraacetate acid (EDTA) > citric acid (CA) > tartaric acid (TA)> oxalic acid (OA). The impact of SO42-, Cl-, CO32- and NO3- was not evident in the release of Cd from the sediment. Besides, Ca(II) and Mg(II) had a major influence on F1 of Cd, and Al(III) and Fe(III) affected various chemical fractions of Cd. The study outcomes provide fundamental knowledge for understanding the mobility and potential ecotoxicological impacts of Cd in sediment.
De novo genome assembly and genome skims reveal LTRs dominate the genome of a limestone endemic Mountainsnail (Oreohelix idahoensis)
Authors: Linscott T. Mason , González-González Andrea , Hirano Takahiro , Parent Christine 
Published: June 2022

Abstract
Background
Calcareous outcrops, rocky areas composed of calcium carbonate (CaCO3), often host a diverse, specialized, and threatened biomineralizing fauna. Despite the repeated evolution of physiological and morphological adaptations to colonize these mineral rich substrates, there is a lack of genomic resources for calcareous rock endemic species. This has hampered our ability to understand the genomic mechanisms underlying calcareous rock specialization and manage these threatened species.
Results
Here, we present a new draft genome assembly of the threatened limestone endemic land snail Oreohelix idahoensis and genome skim data for two other Oreohelix species. The O. idahoensis genome assembly (scaffold N50: 404.19 kb; 86.6% BUSCO genes) is the largest (~ 5.4 Gb) and most repetitive mollusc genome assembled to date (85.74% assembly size). The repetitive landscape was unusually dominated by an expansion of long terminal repeat (LTR) transposable elements (57.73% assembly size) which have shaped the evolution genome size, gene composition through retrotransposition of host genes, and ectopic recombination. Genome skims revealed repeat content is more than 2–3 fold higher in limestone endemic O. idahoensis compared to non-calcareous Oreohelix species. Gene family size analysis revealed stress and biomineralization genes have expanded significantly in the O. idahoensis genome.
Conclusions
Hundreds of threatened land snail species are endemic to calcareous rock regions but there are very few genomic resources available to guide their conservation or determine the genomic architecture underlying CaCO3 resource specialization. Our study provides one of the first high quality draft genomes of a calcareous rock endemic land snail which will serve as a foundation for the conservation genomics of this threatened species and for other groups. The high proportion and activity of LTRs in the O. idahoensis genome is unprecedented in molluscan genomics and sheds new light how transposable element content can vary across molluscs. The genomic resources reported here will enable further studies of the genomic mechanisms underlying calcareous rock specialization and the evolution of transposable element content across molluscs
Asymmetry in the thermal sensitivity of predator and prey performance may explain T-S and Bergmann’s rules in zooplankton
Authors: Maszczyk Piotr , Babkiewicz Ewa , Wilczyński Wojciech , Gliwicz Z. Maciej , Leniowski Konrad , Zebrowski Marcin 
Published: June 2022

Abstract
Numerous studies have demonstrated an inverse correlation between ambient temperature and body size in various ectotherms. This pattern could be explained by the greater temperature effect on the predator’s foraging rate and, in turn, on the mortality rate in comparison to the birth rate in prey populations. While a number of studies have revealed that the latter effect is close to the one anticipated by the Q10 = 2 assumption, the hypothesis would be confirmed if the effect of temperature on the foraging rate of the predator was greater than Q10 = 2. To test this hypothesis, we compared the capture rate and SMR of two fish species (rudd and Malabar danio) and several parameters characterising the mobility of the fish and their planktonic prey (Daphnia) at different temperatures. Although the Q10 for SMR was only around 2, the capture rate increased with rising temperatures much more than expected from the Q10 = 2 assumption, which confirmed our hypothesis. The most likely explanation seems to be a much greater increase of mobility and, in turn, reaction field volume of the fish than of its planktonic prey at elevated temperatures, which improves its attack rate more than predicted by the Q10 = 2 assumption.
Molecular identification of Lingyun Baihao wild and cultivated tea through genome-wide sequencing
Authors: He Shuang , Wang Xiaohu , Yin Meng , Ye Jingping , Meng Jianzong , liqin zhou 
Published: June 2022

Abstract
The tea plants have been one of the most widely consumed and oldest nonalcoholic beverage crops in the world. Lingyun Baihao tea plants are unique species and widely distribute in Guangxi. It is well known that aroma usually determine the quality of tea. In recent years, molecular identification has been employed in the tea plant at the genetic level. However, the association of aroma loss issues to genetic diversities between wild and cultivated tea plants is not clear. Here, we identified 112,938,224 single nucleotide polymorphisms (SNPs) and 10,736,211 insertions/deletions (InDels) for the tea plants through the whole genome sequencing technology. Both SNPs and InDels have certain characteristics in the tea genome, which transition in SNPs type distributes wildly comparing with transversion and single base variation has absolute advantage in InDels. These markers demonstrated that DNA polymorphisms and high diversity. Collectively, we confirmed that the loss of aroma might due to genetic differences at the molecular level between wild and cultivated tea plants, which will be valuable resources for further genetic researches of Lingyun Baihao tea plant.
DNA methylation profile in beef cattle is influenced by additive genetics and age
Authors: Ribeiro Andre Mauric F. , Sanglard Leticia P. , Wijesena Hiruni R. , Ciobanu Daniel C. , Horvath Steve , Spangler Matthew L. 
Published: June 2022

Abstract
DNA methylation (DNAm) has been considered a promising indicator of biological age in mammals and could be useful to increase the accuracy of phenotypic prediction in livestock. The objectives of this study were to estimate the heritability and age effects of site-specific DNAm (DNAm level) and cumulative DNAm across all sites (DNAm load) in beef cattle. Blood samples were collected from cows ranging from 217 to 3,192 days (0.6 to 8.7 years) of age (n = 136). All animals were genotyped, and DNAm was obtained using the Infinium array HorvathMammalMethylChip40. Genetic parameters for DNAm were obtained from an animal model based on the genomic relationship matrix, including the fixed effects of age and breed composition. Heritability estimates of DNAm levels ranged from 0.18 to 0.72, with a similar average across all regions and chromosomes. Heritability estimate of DNAm load was 0.45. The average age effect on DNAm level varied among genomic regions. The DNAm level across the genome increased with age in the promoter and 5' UTR and decreased in the exonic, intronic, 3’ UTR, and intergenic regions. In addition, DNAm level increased with age in regions enriched in CpG and decreased in regions deficient in CpG. Results suggest DNAm profiles are influenced by both genetics and the environmental effect of age in beef cattle.
Acute renal intracystic hemorrhage in patients with autosomal dominant polycystic kidney disease
Authors: Suwabe Tatsuya , Ubara Yoshifumi , Oba Yuki , Mizuno Hiroki , Ikuma Daisuke , Yamanouchi Masayuki , Sekine Akinari , Tanaka Kiho , Hasegawa Eiko , Hoshino Junnichi , Sawa Naoki 
Published: June 2022

Abstract
Renal cyst bleeding is a frequent problem in patients with autosomal dominant polycystic kidney disease (ADPKD). However, there remains minimal information concerning its frequency, causative factors, and effects on ADPKD progression. We investigated the total volume of acute renal intracystic hemorrhage (ARIH) and its relationship to total kidney volume (TKV) in patients with ADPKD on dialysis; we evaluated factors potentially associated with ARIH. We enrolled 199 patients who received renal transcatheter arterial embolization (TAE) from 2014 to 2018 (107 men, 92 women; mean age: 59.1 ± 8.6 years). The median volume of ARIH was 97.3 ml (interquartile range: 36.6–261.7 ml). Multivariate analysis revealed that body weight and ARIH volume were significantly associated with TKV; age, body mass index, smoking, cerebral vascular disease, serum alkaline phosphatase, and TKV were significantly associated with ARIH; and sex, age, dialysis vintage, TKV, and ARIH volume were significantly associated with the number of microcoils required to achieve renal TAE. ARIH was common among ADPKD patients on dialysis; it was significantly associated with TKV. ARIH was greater in younger patients with greater renal artery luminal volume. In conclusion, renal cyst bleeding and renal artery blood flow volume may synergistically accelerate the enlargement of polycystic kidneys.
Photobiomodulation by low-power laser irradiation promotes odontogenic differentiation of stem cells from human exfoliated deciduous teeth
Authors: Wang Ziting , Tian Tian , Chen Leyi , Zhang Chuhan , Zhong Ni , Zheng Xiao , Wu Buling , Xu Wen'an 
Published: June 2022

Abstract
Purpose
The aim of this study was to evaluate the effect of photobiomodulation (PBM) on stem cells from human exfoliated deciduous teeth (SHED) odontogenic differentiation and biomineralization potential at various different energy fluences.
Methods
One control group (without irradiation) and four testing groups (irradiation at 0.5, 5.0 and 10.0 J/cm2 with 10s exposure time) were designed. Cell viability of SHED in response to varying energy fluences of PBM was investigated by the Cell Counting Kit-8 assay. Biomineralization abilitiy of different groups was determined by alkaline phosphatase (ALP) staining and Alizarin Red S (ARS) staining. The qRT-PCR and western blotting were conducted to measure the levels of genes and proteins related to SHED odontogenic differentiation.
Results
PBM at 0.5 J/cm2 significantly promoted SHED proliferation compared to the control group. In addition, PBM at 5.0 J/cm2 significantly upregulated the expression of osteo-/odontogenic genes including runt-related transcription factor 2 (RUNX2), ALP, bone morphogenetic protein 2 (BMP2), dentin sialophosphoprotein (DSPP) and dentin matrix acidic phosphoprotein 1 (DMP1). Moreover, The PBM at 5.0 J/cm2 modulate the components of the BMP/Smad and Wnt signaling pathway involves in BMP2, p-Smad 1/5/9, p-β-catenin and RUNX2.
Conclusion
PBM could trigger SHED odontogenic differentiation and biomineralization in a dose-dependent manner through the activation of the components of the BMP/Smad and Wnt signaling pathway. These results reveal a promising strategy for application of PBM in for vital pulp therapies of deciduous teeth.
Acceptance, Hesitancy, and Refusal in anti-COVID-19 vaccination. A cluster analysis aiming at the typology behind these three concepts
Authors: Cristea Darie , Ilie Dragoș-Georgian , Constantinescu Claudia , Fîrțală Valeriu 
Published: June 2022

Abstract
Most sociological studies on the intention to vaccinate against COVID-19 in the last year have divided the public into people who want to be vaccinated, people who are against vaccination and hesitant. Opinion polls also described how the public relates to specific motivations for agreement, refusal or hesitation about vaccination.Our study proposes a typology of attitude towards vaccination against covid constructed by cluster analysis, based on a battery of questions that indicate the attitude of the subjects towards this vaccination on several dimensions.The questions were deliberately placed by the authors of the study in a nationally representative opinion poll for Romania, in order to collect these data and to study the resulting typology.
Sponge Campus Design by Incorporating Vertical Design and LID Facilities: A Case Study in Eastern China
Authors: Fei Yiming , Fu Dafang , Singh Rajendra Prasad , Shang Qingyu 
Published: June 2022

Abstract
With the rapid advancement of sponge city infrastructure in China, the concept and construction of sponge campus have received wide attention. Implementation of a sponge campus incorporated with low impact development (LID) facilities could effectively reduce runoff and promote rainwater recycling in case of rainfall occurrence, provide resilience and improve stability against climate change for both system itself and adjacent regions. However, most of the studies on sponge campus in China are focused on the design and evaluation of college campuses, while little emphasis has been put on elementary and secondary schools where the requirement and characteristics of target users are totally different. In this paper, designing concepts regarding vertical design and application of LID for sponge campus for elementary and secondary schools were given based on engineering practice. Then a typical secondary school campus in Kunshan, China was taken as an example, and simulation was conducted on recurrence period of 1 year and 20 years using stormwater management model (SWMM). The results showed that, under the rainfall intensity with a recurrence period of 1 year and 20 years, the peak flow was reduced 46.8% and 59.0%, respectively, while the total inflow was reduced 25.4% and 33.9%, respectively. Under recurrence period of 1 year, this vertically-designed campus with LID facilities provided 15.13% water storage capacity for the neighboring plot with only 9.16% of the plot area. This new way of vertical design coupled with LID facilities can help to create a much more ecological, safe and beautiful school campus.
Marker based monitoring for virulence distribution of Pseudomonas syringae pv. tomato causing bacterial speck diseased of tomato in Egypt
Authors: El-Fatah Bahaa E.S. Abd , Abo-Elyousr Kamal A.M , Mahmoud Amer F. , Imran Muhammad 
Published: June 2022

Abstract
The bacterial pathogen Pseudomonas syringae pv. tomato is the most important pathogen causing bacterial speck disease of tomato. Present study was conducted to investigate and characterize the population diversity of Pseudomonas syringae pv. tomato pathogen collected from infected tomato growing regions of Egypt. Significant variation was observed among the five isolates which showed significant level of virulence. Genetic variation among the isolates was investigated by PCR approach for the amplification of hrpZ gene using random amplified polymorphic DNA (RLFP), sequence-related amplified polymorphism (SRAP), and inters simple sequence repeats (ISSR). The length of amplified product for ITS1 region was 810 bp whereas for hrpZ gene was 536bp with primer pairs (1406-f/23S-r) and (MM5-F, MM5-R) respectively. The restriction analysis of amplified regions “ITS” and hrpZ by using 5 and 4 endonucleases respectively, showed slight variation among the bacterial isolates. The results of RAPD, ISSR and SRAP showed higher polymorphism (60.52%) between the isolates which assist for their successful characterization by unique and specific positive markers, based on geographical distribution, origin and virulence intensity. The results of this study suggested that the use of molecular approach can provide successful and valuable information for differentiation and classification of Pseudomonas syringae pv. tomato in future.
Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China
Authors: Wu Zun , Wu Yating , Gao Haofeng , He Xuexin , Yao Qiang , Yang Zhanglei , Zhou Jinyi , Ji Linting , Gao Jinwei , Jia Xuying , Dou Yong , Wang Xiaoyu , Shao Peng 
Published: June 2022

Abstract
Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The scales of the diseased fish fell off, water was accumulated in their abdomens, and their tissues and organs were damaged. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity.