bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Ion‐Enhanced Oncolytic Virus‐Like Nanoparticle for Tumor Immunotherapy
Authors: Wu Fan , Li Yanli , Meng Yun , Cai Xuechao , Shi Jieyun , Li Jinjin , Chen Yang , Zhang Li , Meng Xianfu , Li Huiyan , Jiang Xingwu , Fu Zhenxiao , Wu Yelin , Bu Wenbo 
Published: September 2022

AbstractT lymphocytes (T cells) are essential for tumor immunotherapy. However, the insufficient number of activated T cells greatly limits the efficacy of tumor immunotherapy. Herein, we proposed an oncolytic virus‐mimicking strategy to enhance T cell recruitment and activation for tumor treatment. We constructed an oncolytic virus‐like nanoplatform (PolyIC@ZIF‐8) that was degraded in the acidic tumor environment to release PolyIC and Zn2+. The released PolyIC exhibited an oncolytic virus‐like function that induced tumor cell apoptosis and promoted T cell recruitment and activation through a tumor antigen‐dependent manner. More importantly, the released Zn2+not only enhanced T cell recruitment by inducing CXCL9/10/11 expression but also promoted T cell activation to increase interferon‐γ (INF‐γ) expression by inducing the phosphorylation of ZAP‐70 via a tumor antigen‐independent manner. This Zn2+‐enhanced oncolytic virus‐mimicking strategy provides a new approach for tumor immunotherapy.
Regio‐ and Diastereoselective Formal [2+2] Cycloaddition of Allenes with Amino‐Functionalized Alkenes by Rare‐Earth‐Catalyzed C(sp2)−H Activation
Authors: Xu Wenxuan , Cong Xuefeng , An Kun , Lou Shao‐Jie , Li Zhenghua , Nishiura Masayoshi , Murahashi Tetsuro , Hou Zhaomin 
Published: September 2022

AbstractThe [2+2] cycloaddition of allenes with alkenes is of much interest and importance as a straightforward route for the construction of four‐membered carbocycles but has remained much underexplored to date. Herein we report for the first time the intermolecular regio‐ and diastereoselective formal [2+2] cycloaddition of a wide range of allenes with amino‐functionalized alkenes by half‐sandwich rare‐earth catalysts. The reaction proceeded through an allene C(sp2)−H activation mechanism initiated by the site‐selective deprotonation of the allene unit by a rare‐earth metal alkyl species followed by alkene insertion into the resulting metal‐allenyl bond and the subsequent intramolecular cycloaddition to an allene C=C bond. This protocol offers a unique route for the synthesis of a new family of cyclobutane and cyclobutene derivatives which were difficult to access previously.
19F Barcoding Enables Multiplex Detection of Biomarkers Associated with Organ Injury and Cancer
Authors: Luo Xiangjie , Kang Bilun , Chi Xiaoqin , Xiong Hui , Chen Dongxia , Fan Yifan , Li Lingxuan , Chen Limin , Li Ao , Gao Jinhao , Lin Hongyu 
Published: September 2022

AbstractSimultaneous detection of multiple biomarkers in complex environments is critical for the in‐depth exploration of different biological processes, which is challenging for many current analytical methods due to various limitations. Herein, we report a strategy of 19F barcoding which takes the advantages of 19F's high magnetic resonance (MR) sensitivity, prompt signal response to environmental changes, negligible biological background, quantitative signal output, and multiplex capacity. A set of 19F‐barcoded sensors responding to different biomarkers involved in organ injury and cancer are designed, synthesized, and characterized. With these sensors, we accomplish concurrent assessment of different biomarkers in the samples collected from the mice with drug‐induced liver/kidney injury or tumor, illustrating the feasibility of this approach for multiplexed detection of different biomarkers in complex environments during various biological processes.
Quantifying Reductive Amination in Nonenzymatic Amino Acid Synthesis
Authors: Mayer Robert J. , Moran Joseph 
Published: September 2022

AbstractAmino acid biosynthesis initiates with the reductive amination of α‐ketoglutarate with ammonia to produce glutamate. However, the other α‐keto acids derived from the glyoxylate and Krebs cycles are converted into amino acids by transamination, rather than by reductive amination. Why is only one amino acid synthesized by reductive amination and not the others? To explore this question, we quantified the inherent reactivities of keto acids in nonenzymatic reduction and reductive amination by using BH3CN− as a model nucleophile. Biological α‐keto acids were found to show pronounced nonenzymatic reactivity differences for the formation of amino acids (α‐ketoglutarate<oxaloacetate≈pyruvate≪glyoxylate). Accordingly, the flow of ammonia passes through the least reactive α‐keto acid of the Krebs cycle. One possible explanation for this choice is the position of the corresponding amino acid, glutamate, at the top of the thermodynamic landscape for subsequent transamination reactions.
Synthesis and biological evaluation of coumarin‐thiazole hybrids as selective carbonic anhydrase IX and XII inhibitors
Authors: Thacker Pavitra S. , Newaskar Vaishnavi , Angeli Andrea , Sigalapalli Dilep Kumar , Goud Nerella Sridhar , Chirra Hepsibha , Shaik Afzal B. , Arifuddin Mohammed , Supuran Claudiu T. 
Published: September 2022

AbstractA series of coumarin‐linked thiazoles (6a–p) was synthesized and the synthesized compounds were evaluated against human carbonic anhydrases (hCAs) IX and XII, which have been implicated in cancer. All the compounds exhibited selective inhibition of both isoforms. The designed compounds inhibited hCA IX in a moderate nanomolar to submicromolar range. The hCA XII was inhibited in a low to moderate nanomolar range. Compound 6o exhibited the best inhibition of hCA XII with a Ki value of 91.1 nM. The hydrolyzed form of compound 6o also exhibited favorable interactions as well as good docking scores with both the isoforms. Hence, this compound can be taken as a template for the design of selective and potent hCA XII inhibitors.
Dynamic Prediction of Pulmonary Hypertension in Systemic Sclerosis Using Landmark Analysis
Authors: Nihtyanova Svetlana I. , Schreiber Benjamin E. , Ong Voon H. , Wells Athol U. , Coghlan John G. , Denton Christopher P. 
Published: September 2022

ObjectivePulmonary hypertension (PH) is a serious complication of systemic sclerosis (SSc). In this study, we explored the prediction of short‐term risk for PH using serial pulmonary function tests (PFTs) and other disease features.MethodsSSc patients in whom disease onset occurred ≥10 years prior to data retrieval and for whom autoantibody specificity and PFT data were available were included in this study. Mixed‐effects modeling was used to describe changes in PFTs over time. Landmarking was utilized to include serial assessments and stratified Cox proportional hazards regression analysis with landmarks as strata was used to develop the PH prediction models.ResultsWe analyzed data from 1,247 SSc patients, 16.3% of whom were male and 35.8% of whom had diffuse cutaneous SSc. Anticentromere, antitopoisomerase, and anti–RNA polymerase antibodies were observed in 29.8%, 22.0%, and 11.4% of patients, respectively, and PH developed in 13.6% of patients. Over time, diffusing capacity for carbon monoxide (DLco) and carbon monoxide transfer coefficient (Kco) declined in all SSc patients (up to 1.5% per year) but demonstrated much greater annual decline (up to 4.5% and 4.8%, respectively) in the 5–7 years preceding PH diagnosis. Comparisons between multivariable models including either DLco, Kco, or forced vital capacity (FVC)/DLco ratio, demonstrated that both absolute values and change over the preceding year in those measurements were strongly associated with the risk of PH (hazard ratio [HR] 0.93 and 0.76 for Kco and its change; HR 0.90 and 0.96 for DLco and its change; and HR 1.08 and 2.01 for FVC/DLco ratio and its change; P < 0.001 for all). The Kco‐based model had the greatest discriminating ability (Harrell's C‐statistic 0.903).ConclusionOur findings strongly support the importance of PFT trends over time in identifying patients at risk of developing PH.
Preparation and characterization of biogenic silver nanoparticles using Strobilanthes cordifolia (Vahl) J.R.I. Wood leaves and its biological applications
Authors: Kirubakaran Dharmalingam , Selvam Kuppusamy , Prakash Palanisamy , Manimegalai Peraman , Shivakumar Muthugounder Subaramanian , SenthilNathan Sengottayan 
Published: September 2022

AbstractIn the present study, aqueous leaf extract of Strobilanthes cordifolia J.R.I.Wood was combined with silver nitrate to synthesis silver nanoparticles (AgNPs). The AgNPs were characterized using visible spectroscopy (UV), X‐ray diffraction (XRD), Fourier transform infrared spectrophotometer (FTIR), scanning electron microscope (SEM), energy dispersive X‐ray (EDaX), particle size analysis, and transmission electron microscope (TEM). The UV spectrum absorption peak occurred at 438 nm. The FTIR analysis of the AgNPs indicated the presence of functional groups such as aldehyde, alkenes, and carboxylic acids. The crystalline structure of AgNPs was confirmed by XRD. The AgNPs have a spherical shape according to SEM. The AgNPs components composition was confirmed by EDaX. The particle size distribution of AgNPs is monodispersion in the range at 42.54 nm. TEM demonstrated the AgNPs size to be between 11.35 and 34.90 nm. The AgNPs exhibited good antibacterial property against Escherichia coli and Staphylococcus aureus. The antioxidant activity of the AgNPs was represented by increased DPPH, ABTS, and H2O2 activities.The antidiabetic activity of the AgNPs was indicated by the inhibition of α‐amylase and α‐glycosidase and anti‐inflammatory highest albumin denaturation and HRBC membrane stabilization properties. Further, the AgNPs also significantly inhibited the MCF‐7 cell lines. These results clearly suggest that the synthesized AgNPs using S. cordifolia leaves could have several potential biomedical applications.
Ammonium‐Functionalized Naphthalene Diimides as Two‐Electron‐Transfer Negolyte for Aqueous Redox Flow Batteries
Authors: Singh Vikram , Ahn Seongmo , Byon Hye Ryung 
Published: September 2022

AbstractAqueous organic redox flow batteries (AORFBs) have been developed as safe and economical energy storage systems for renewable energy applications. Herein, the solubility of two‐electron‐transfer organic molecules, naphthalene diimides (NDIs), was improved by adding two propyl‐spaced ammonium functionalities. Under neutral conditions, the di‐ammonium‐functionalized NDIs exhibited a solubility of ∼0.7 M (1.4 M/e−) in water. Using 0.3 M NDIs as negolyte (negative electrolyte) and iodide/triiodide as posolyte (positive electrolyte), AORFBs achieved 300 galvanostatic cycles with approximately 100 % capacity retention. The post‐mortem analyses revealed negligible chemical decomposition with no crossover while using a Nafion membrane. This study presents a promising NDI negolyte that can achieve stable two‐electron transfer in AORFBs.
Pilot scale production and characterization of next generation high molecular weight and tense quaternary state polymerized human hemoglobin
Authors: Cuddington Clayton T. , Wolfe Savannah R. , Belcher Donald A. , Allyn Megan , Greenfield Alisyn , Gu Xiangming , Hickey Richard , Lu Shuwei , Salvi Tanmay , Palmer Andre F. 
Published: September 2022

AbstractPolymerized human hemoglobin (PolyhHb) is being studied as a possible red blood cell (RBC) substitute for use in scenarios where blood is not available. While the oxygen (O2) carrying capacity of PolyhHb makes it appealing as an O2 therapeutic, the commercial PolyhHb PolyHeme® (Northfield Laboratories Inc.) was never approved for clinical use due to the presence of large quantities of low molecular weight (LMW) polymeric hemoglobin (Hb) species (<500 kDa), which have been shown to elicit vasoconstriction, systemic hypertension, and oxidative tissue injury in vivo. Previous bench‐top scale studies in our lab demonstrated the ability to synthesize and purify PolyhHb using a two‐stage tangential flow filtration purification process to remove almost all undesirable Hb species (>0.2 µm and <500 kDa) in the material, to create a product that should be safer for transfusion. Therefore, to enable future large animal studies and eventual human clinical trials, PolyhHb synthesis and purification processes need to be scaled up to the pilot scale. Hence in this study, we describe the pilot scale synthesis and purification of PolyhHb. Characterization of pilot scale PolyhHb showed that PolyhHb could be successfully produced to yield biophysical properties conducive for its use as an RBC substitute. Size exclusion high performance liquid chromatography showed that pilot scale PolyhHb yielded a high molecular weight Hb polymer containing a small percentage of LMW Hb species (<500 kDa). Additionally, the auto‐oxidation rate of pilot scale PolyhHb was even lower than that of previous generations of PolyhHb. Taken together, these results demonstrate that PolyhHb has the ability to be seamlessly manufactured at the pilot scale to enable future large animal studies and clinical trials.
Personalisation methods in e‐learning‐A literature review
Authors: Mikić Vladimir , Ilić Miloš , Kopanja Lazar , Vesin Boban 
Published: September 2022

AbstractOver the years, personalisation in e‐learning has evolved as a promising paradigm for matching learners' specific requirements. Although many literature reviews on personalised e‐learning have been conducted, there are still voids and limitations in the comprehensive literature survey regarding how personalisation might occur in e‐learning environments and benefit the teaching and learning process. In addition, no design guidelines, frameworks, or tools to guide its implementation exist, so practice can vary widely. The principal purpose of this literature review is to examine the subject, provide insights into the use of personalised techniques and systems in e‐learning, and investigate its impact on the engagement and success of the students. This study applies methods of precise selection criteria to determine which of the selected publications have the strongest interconnection and relevance with the topic of e‐learning personalisation. It also categorises the personalisation techniques employed by each identified system and highlights new perspectives and advances in the area.
Smart generation of code tracing questions for assessment in introductory programming
Authors: Stankov Emil , Jovanov Mile , Madevska Bogdanova Ana 
Published: September 2022

AbstractTeaching programming is a challenging activity nowadays, especially in introductory programming courses, which are typically massively attended. Writing functional programs is a cognitive skill, which many students, novices in programming, find it difficult to master. It is equally challenging to assess this ability of students. Research has shown that students need to learn how to read and understand, before they can learn to write programs. Code tracing questions are a suitable way to assess the knowledge of semantics of programming constructs (understanding what programs do). However, when designing these types of questions, teachers need to be aware of the complexity of the code and must always try to create questions with code snippets of same or similar complexity, especially when preparing different versions of the same test (for large student groups). In this paper we propose a new model for smart automatic generation of code tracing questions. The model uses a suitable source code metric to measure the code complexity, and to enable generation of questions containing code snippets of consistent complexity. We also introduce CodeCPP: a newly developed Moodle plugin, which is an implementation of the proposed model. The evaluation of CodeCPP, through a year‐long use of the system in teaching programming as well as feedback from selected teachers with many years of experience in teaching programming courses, shows positive and promising results. The system utilization confirms that the application of the proposed model enables a fair and objective knowledge assessment. Furthermore, the feedback provided by teachers and students is highly positive.
Microarray and in silico analysis of DNA repair genes between human testis of patients with nonobstructive azoospermia and normal cells
Authors: Hashemi Karoii Danial , Azizi Hossein , Skutella Thomas 
Published: September 2022

AbstractDNA repair processes are critical to maintaining genomic integrity. As a result, dysregulation of repair genes is likely to be linked with health implications, such as an increased prevalence of infertility and an accelerated rate of aging. We evaluated all the DNA repair genes (322 genes) by microarray. This study has provided insight into the connection between DNA repair genes, including RAD23B, OBFC2A, PMS1, UBE2V1, ERCC5, SMUG1, RFC4, PMS2L5, MMS19, SHFM1, INO80, PMS2L1, CHEK2, TRIP13, and POLD4. The microarray analysis of six human cases with different nonobstructive azoospermia revealed that RAD23B, OBFC2A, PMS1, UBE2V1, ERCC5, SMUG1, RFC4, PMS2L5, MMS19, SHFM1, and INO80 were upregulated, and expression of PMS2L1, CHEK2, TRIP13, and POLD4 was downregulated versus the normal case. For this purpose, Enrich Shiny GO, STRING, and Cytoscape online evaluation was applied to predict proteins’ functional and molecular interactions and then performed to recognize the master pathways. Functional enrichment analysis revealed that the biological process (BP) terms “base‐excision repair, AP site formation,” “nucleotide‐excision repair, DNA gap filling,” and “nucleotide‐excision repair, preincision complex assembly” was significantly overexpressed in upregulated differentially expressed genes (DEGs). BP analysis of downregulated DEGs highlighted “histone phosphorylation,” “DNA damage response, detection DNA response,” “mitotic cell cycle checkpoint signaling,“ and “double‐strand break repair.” Overrepresented molecular function (MF) terms in upregulated DEGs included “Oxidized base lesion DNA N‐glycosylase activity,” “uracil DNA N‐glycosylase activity,” “bubble DNA binding” and “DNA clamp loader activity.” Interestingly, MF investigation of downregulated DEGs showed overexpression in “heterotrimeric G‐protein complex,” “5′‐deoxyribose‐5‐phosphate lyase activity,” “minor groove of adenine‐thymine‐rich DNA binding,” and “histone kinase activity.” Our findings suggest that these genes and their interacting hub proteins could help determine the pathophysiology of germ cell abnormalities and infertility.
Outcomes and challenges of the provisional stenting technique: Insights from the PROGRESS‐BIFURCATION registry
Authors: Simsek Bahadir , Kostantinis Spyridon , Karacsonyi Judit , Allana Salman , Vemmou Evangelia , Nikolakopoulos Ilias , Burke Martin Nicholas , Garcia Santiago , Wang Yale , Chavez Ivan , Gössl Mario , Sorajja Paul , Mooney Michael , Poulose Anil , Sandoval Yader , Traverse Jay , Rangan Bavana V. , Brilakis Emmanouil S. 
Published: September 2022

AbstractBackgroundProvisional stenting is the most commonly used coronary bifurcation stenting strategy.MethodsWe examined the clinical and angiographic characteristics, challenges encountered, and procedural outcomes with the provisional bifurcation stenting strategy in the Prospective Global Registry of Percutaneous Coronary Intervention (PCI) in Bifurcation Lesions (PROGRESS‐BIFURCATION, NCT05100992).ResultsProvisional stenting was used in 334 of 430 bifurcation interventions (78%). Technical success was lower (95% vs. 100%, p = 0.017) in provisional, but procedural success (90% vs. 95%, p = 0.095) and incidence of in‐hospital major adverse cardiovascular events were similar (5% vs. 5%, p = 0.945) compared with two‐stent strategies. Provisional was less often preferred in left main/left anterior descending involvement (47% vs. 73%, p < 0.001). Provisional stenting cases had smaller side branch (SB) diameter (2.4 ± 0.5 vs. 2.7 ± 0.6 mm, p < 0.001), shorter SB lesion length (5 [3−8] vs. 10 [5−10] mm, p < 0.001), less SB diameter stenosis (46 ± 35 vs. 81 ± 20%, p < 0.001), and were less likely to be Medina 1,1,1 (34% vs. 73%, p < 0.001). PCI challenges were less common (30% vs. 58%, p < 0.001) with provisional stenting: (1) rewiring difficulty (43%) that was overcome with use of a different wire (74%) or microcatheter (46%); (2) inability to deliver a stent (22%) or balloon (9%) that was overcome with use of a smaller balloon (88%), rewiring (25%), or increased support/microcatheter (25%).ConclusionsProvisional bifurcation stenting was more often performed in distal lesions with less SB involvement and had lower technical success, but similar procedural success and complications compared with two‐stent strategies.
Successful treatment of severe post hematopoietic stem cell transplantation bronchiolitis obliterans syndrome with lung transplantation in a patient with multi‐organ chronic graft‐versus‐host disease
Authors: Pénichoux Juliette , Bouclet Florian , Alani Mustafa , Contentin Nathalie , Ménard Anne‐Lise , Leprêtre Stéphane , Lenain Pascal , Stamatoullas Aspasia , Lhuillier Elodie , Lanic Hélène , Lemasle Emilie , Dubois Sydney , Bourhis Jean‐Henri , Mal Hervé , Jardin Fabrice , Camus Vincent 
Published: September 2022

AbstractAllogeneic stem cell transplant and chronic graft‐versus‐host disease may lead to severe non‐infectious pulmonary disease in 6% of patients at 5 years. We report the case of a young patient with acute myeloid leukemia who successfully received bilateral lung transplantation for severe bronchiolitis obliterans syndrome.
Pseudohexagonal Nb2O5‐Decorated Carbon Nanotubes as a High‐Performance Composite Anode for Sodium Ion Batteries
Authors: Chen Guanxu , Chen Jintao , Parkin Ivan P. , He Guanjie , Miller Thomas S. 
Published: September 2022

AbstractTo promote the commercial application of sodium ion batteries (SIBs), new high‐stability and high‐capacity anode materials are needed. Metal oxides show great promise, but significant issues relating to their low electrical conductivity and unstable structure during charge storage must be resolved. Herein, a new composite anode consisting of pseudohexagonal Nb2O5 (TT‐Nb2O5) nanoparticles strongly anchored to carbon nanotubes (CNT) through a glucose‐derived carbon framework (Nb2O5/g‐CNT) was synthesised and assessed for use as the anode in SIBs. This represents the first application of TT‐Nb2O5 in SIBs. The composite is shown to offer high specific capacity (203 mAh g−1 at 0.2 A g−1), good rate capability (53 mAh g−1 at high current density of 5 A g−1), and a high‐capacity retention rate (135 mAh g−1 at 0.2 A g−1 between 25 and 300 cycles). This is attributed to high electrical conductivity and flexibility offered by the designed carbon framework, the superior capacity of the TT‐Nb2O5 and the linkage between the two provided by the bonding glucose derived carbon. This work therefore demonstrates a scalable route for the application of metal oxides in future high‐performance SIB systems.
Initial Stages of Sodium Deposition onto Au(111) from [MPPip][TFSI]: An In‐Situ STM Study for Sodium‐Ion Battery Electrolytes
Authors: Heubach Maren‐Kathrin , Schuett Fabian M. , Kibler Ludwig A. , Abdelrahman Areeg , Jacob Timo 
Published: September 2022

AbstractInvited for this issue's Front Cover is the group of Professor Timo Jacob at Ulm University. The cover picture shows a scanning tunnelling microscopy (STM) tip that is scanning the Au(111) single crystal surface in‐situ during sodium deposition. Read the full text of the Research Article at 10.1002/celc.202200722.