bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interferon-γ secreted by recruited Th1 cells in peritoneal cavity inhibits the formation of malignant ascites
Authors: SONG Yu , Liu Chang , Xiao Zhuanglong , Du Li , Zhu Shenghua , Xiang Hongyu , Wang Zehui , Liu Fang 
Published: November 2022

Abstract
Type1 T helper (Th1) cells generate an efficient antitumor immune response in multiple malignancies. The functions of Th1 cells in malignant ascites (MA) have not been elucidated. The distribution of helper T cells in peritoneal fluid and peripheral blood was determined in patients and animal model with malignant ascites. The effects of Th1-derived interferon-γ (IFN-γ) on the formation of malignant ascites were investigated. The mechanism underlying the recruitment of Th1 cells into peritoneal cavity was explored. In patients with malignant ascites and animal models of malignant ascites, the percentage of Th1 cells increased in peritoneal fluid compared with peripheral blood. Next, our experiment demonstrated that Th1 cells inhibited the growth of tumor cells by secreting IFN-γ in vitro. In murine models of malignant ascites, increased peritoneal fluid and shorter survival time were observed in IFN-γ−/− mice compared with WT mice. Then, the level of C-X-C motif chemokine ligand (CXCL) 9/10 and the ratio of CXCR3+ Th1 cells indicated the involvement of CXCL9, 10/CXCR3 axis in the recruitment of Th1 cells into peritoneal cavity. As expected, in murine models of malignant ascites, the gradient between ascites Th1 ratio and blood Th1 ratio decreased in CXCR3−/− mice compared with WT mice. IFN-γ secreted by recruited Th1 cells in peritoneal cavity inhibits the formation of malignant ascites. Hence, manipulation of Th1 cells or IFN-γ will provide a therapeutic candidate against malignant ascites.
Proposition and Application of Multi-criteria Analysis With Fuzzy Logic Approach and Consequential Life Cycle Assessment to Support Decision-making on Life Cycle Sustainability
Authors: Maranduba Henrique Leonardo , Cruz Rosenira Serpa da , Rodrigues Luciano Brito , Neto José Adolfo de Almeida 
Published: November 2022

Abstract

The purpose of this study is to use a multicriteria fuzzy controller (MFC) associated with the cLCA to assist in the sustainability assessment, including different perspectives (environmental, exergetic, and economic), allowing more objectivity and confidence in interpreting results. The case used in this study to test the purpose uses a cradle-to-grave approach with a system expansion boundary (avoided burdens) of transporting one ton of cargo by one kilometer, with diesel B, using four ReCipe Midpoint categories (H) and the Cumulative Exergy Demand – CExD in the impact assessment. Three representative variables were chosen to test the integrated approach, two LCIA categories (CC and CExD) and one economic (Price). The results presented an approach that integrates cLCA and Fuzzy Logic, solving the limitations mentioned and providing a decision-making tool that can receive inputs from any Triple Bottom Line dimension and generate objective results. When used autonomously, the cLCIA presents results with trade-offs, categories with positive (CC, FE, and ALO), negative (CExD), or neutral impacts (NLT). When combined with Fuzzy Logic, the results bring surfaces, and response areas that allow decision-makers to visualize the limits and trends of the best scenarios, facilitating consensus and managing conflicts. The association of fuzzy controller LCA tools enabled more direct and objective results and findings to support decision-making, integrating different sustainability perspectives.
Microbial degradation of organophosphorous compounds by Soil bacterial isolates
Authors: Chauhan Vinita , Dhaked Ram Kumar 
Published: November 2022

Abstract
Several bacterial strains that can use organophosphates as a source of carbon have been isolated from the soil, where the synthesis of organophosphorus compound takes places. The enrichment of biodegrading bacteria for OP compound was carried out in mineral salt (MS1) medium. All these organisms synthesize an enzyme called organophosphorus hydrolase in each case the enzyme is encoded by a opd gene. The isolates were grouped together using pyro GC, PAGE and identified by 16S rRNA sequencing. Two species of bacteria which are degrading OP compound effectively were identified as sp. of Pseudomonas and Acinetobactor. Organophosphates were utilized second preferred after glucose as carbon source by these bacteria whereas acetate and lactose exhibited minimum effect on growth. Degradation of organophosphorus compound by the bacterial isolates was determined by the estimation of concentration of inorganic phosphorus after 12 and 24 h. The biodegradation of methyl parathion, dimethyl methylphosphonate (DMMP), and sarin proceeded rapidly with the formation of a series of intermediate product, which were analyzed using a combination of GC/MS.
Human GLP1R variants affecting GLP1R cell surface expression contribute to impaired glucose control and increased adiposity
Authors: gao wenwen , liu lei , Huh Eunna , Hegron Alan , Fan Zhiran , Hou Guofei , Charpentier Guillaume , Derhourhi Mehdi , Marre Michel , Balkau Beverley , Froguel Philippe , Lichtarge Olivier , Dam Julie , Bonnefond Amélie , Liu Jianfeng , Jockers Ralf 
Published: November 2022

Abstract
The glucagon-like peptide 1 receptor (GLP1R) is a major drug target with several agonists being prescribed in patients with type 2 diabetes (T2D) and obesity. The impact of genetic variability of the GLP1R gene on receptor function and its association with metabolic traits are unclear. Here, functional profiling of 59 rare and one common GLP1R variant across four signaling pathways reveals an unexpected diversity of phenotypes ranging from defective cell surface expression to complete or pathway-specific gain- and loss-of-functions. Defective insulin secretion of loss-of-function GLP1R variants was rescued by allosteric GLP1R ligands or high exendin-4 concentrations in INS-1 823/3 cells. Genetic association studies in 200K participants from UK Biobank show that impaired GLP1R cell surface expression contributes to impaired glucose control and increased adiposity with increased HbA1c, BMI and diastolic blood pressure. This study defines impaired GLP-1R cell surface expression as a risk factor for T2D- and obesity-associated traits.
Effects of Nonlinear Behaviour of Linear Ball Guideway on Chatter Frequency of Lathe Machine Tool
Authors: Hadraba Petr , Wang Jiunn-Jyh , Hadas Zdenek 
Published: November 2022

Abstract

Linear ball guideways (LBGs) have several advantages for the precise positioning control of machine tools. However, the ball-groove contact behaviour leads to poor dynamic compliance, which induces a significant nonlinear behaviour of the guideway and a poor chatter stability. Accordingly, the present study first employs Hertzian contact theory to describe the ball-groove contact behaviour in the LBG of a lathe machine. Two static models are then derived to predict the LBG stiffness under different cutting loads. For simplicity, the first model ignores the effects of the contact force on the deformation of the guideway components. By contrast, the second model adopts a dynamic sub-structuring co-simulation method to incorporate the effects of structural deformation into the analysis. For both models, a linearisation approach is employed to analyse the dynamic behaviour of the system under static loading and define the dynamic compliance. The chatter frequencies of the LBG are estimated using the second model and are shown to be in good agreement with the experimental results. In general, the analysis results show that fundamental shifts in the frequency response of the LBG occur under specific values of the cutting force and load. In other words, the results confirm the need to take the nonlinear behaviour of the LBG structure into account in the design of lathe machine tools.
Individualized flow-controlled versus conventional pressure-controlled ventilation in on-pump heart surgery (FLOWVENTIN HEARTSURG): study protocol for a randomized controlled trial
Authors: Becker Simon , Schnitzler Romina , Rembecki Martin , Geppert Johannes , Kurz Christian Tobias , Wichelhaus Lisa-Marie , Timmesfeld Nina , Zahn Peter 
Published: November 2022

Abstract
• Background:

In on-pump cardiac surgery lungs are at high risk of periprocedural organ impairment because of atelectasis formation, ventilator-induced lung injury and hyperinflammation due to the cardiopulmonary bypass which results in postoperative pulmonary complications in half of this patient population. The new ventilation mode flow-controlled ventilation (FCV) uniquely allows full control of ins- and expiratory airway flows. This approach reduces mechanical power of invasive ventilation as a possible cause of ventilator-induced lung injury. The scope of FLOWVENTIN HEARTSURG is to compare perioperative individualized FCV with best clinical practice pressure-controlled ventilation (PVC) modes in patients with elective on-pump cardiac surgery procedures. We hypothesize that the postoperative inflammatory response can be reduced by perioperative application of FCV compared to PCV.

• Methods:

FLOWVENTIN HEARTSURG is a single center, randomized, parallel group trial with two intervention arms: perioperative PCV modes (n = 70, PCV group) with an individualized positive end-expiratory pressure (PEEP) and a tidal volume of 6–8 ml/kg predicted bodyweight compared to perioperative FCV (n = 70, FCV group) with an individualized PEEP and driving pressure, resulting in a liberal tidal volume. As the primary study endpoint interleukin 8 plasma level is assessed six hours after cardiopulmonary bypass as a surrogate biomarker of systemic and pulmonary inflammation. As secondary aims clinically relevant patient outcomes are analyzed, e.g. perioperative lung function regarding oxygenation indices, postoperative pulmonary and extra-pulmonary complications, SIRS-free days as well as ICU and total inpatient stays. As additional sub studies with an exploratory approach perioperative right ventricular function parameters are assessed by echocardiography and perioperative lung aeration by Electrical Impedance Tomography.

• Discussion:

Current paradigms regarding protective low tidal volume ventilation are consciously left in the FCV intervention group in order to reduce mechanical power as a determinant of ventilator-induced lung injury in this high-risk patient population and procedures. This approach will be compared in a randomized-controlled trial with current best clinical practice PCV in FLOWVENTIN HEARTSURG.

Trial registration:

German Clinical Trials Register, DRKS00018956. Registered on 12 June 2020 (Version 1), last update on 22 August 2022 (Version 4), https://www.drks.de/drks_web/navigate.do?navigationId=trial.HTML&TRIAL_ID=DRKS00018956.
Vision Rehabilitation Using Microperimetric Biofeedback Training in Age- Related Macular Degeneration
Authors: Widihastha Sri Hudaya , Iskandar Erwin , Satari Karmelita , Irfani Irawati , Virgana Rova , Amiruddin Primawita Oktarima 
Published: November 2022

Abstract
Background
Age-related macular degeneration (AMD) is a degenerative disease affecting retinal tissue that may cause central vision loss. Microperimetric Biofeedback Training (MBFT) is a visual rehabilitation to train patients who have lost foveal fixation to relocate the locus to an area of the retina with better sensitivity.
Objective
To determine the impact of MBFT on the quality of vision in patients with AMD.
Methods
This study was a prospective, interventional, comparative study with subjects of patients diagnosed with AMD in the National Eye Center Cicendo Eye Hospital. Patients were randomly divided into two groups, intervention and non-intervention, with balance block randomization. The intervention group would receive six MBFT training sessions of 10-minute time duration each.
Results
The number of subjects enrolled in this study was 36, with 18 patients in each group. A statistically significant improvement of best corrected visual acuity (BCVA) was found after the intervention, from 1.24 ± 0.416 logMar to 0.83 ± 0.242 (P < 0.001). A statistically significant improvement for near vision acuity (NVA) was also observed, from 1.02 ± 0.307 logMar to 0.69 ± 0.278 logMar (P < 0.001). In addition, reading rate increased, from 40.83 ± 30,411 words/minute to 65.06 ± 31,598 words/minute (P < 0.001). Similarly, a comparison of changes in BCVA, NVA, and reading rate between intervention and non-intervention groups showed a significant difference (P < 0.001).
Conclusion
Microperimetric biofeedback training significantly and positively impacts visual acuity, near vision acuity, and reading rate in patients with AMD.
Ovarian Inactivity in Traditionally Raised Indonesian Bligon Goats: Association with Microminerals
Authors: Widiyono Irkham 
Published: November 2022

Abstract

This study was aimed to investigate the ovarian activity and its association with serum micromineral level in Indonesian Bligon goats. The observations were conducted on non-pregnant Bligon goat does, 2–3 years old, with poor to moderate body condition (Body Condition Score, BCS 1–3 on a scale of 5), reared traditionally, and slaughtered in a slaughterhouse in Yogyakarta Indonesia during the period of April – August (dry season). All animals did not show any gynecological and systemic disease. Before slaughtering, venous blood samples were taken for blood chemistry. After slaughtering, the ovaries were immediately removed for examination of the follicles and the corpus luteum. Zinc (Zn) and copper (Cu) in serum were analyzed by Atomic Absorbent Spectrophotometry. There were 9 animals with inactive ovaries (IO). As a control, 16 animals with active ovaries (AO) were observed. The IO animals had serum Zn level of 2.30 ± 1.17 ppm, while AO had a significantly higher one, 3.64 ± 1.68 ppm (p < 0.05). The serum Cu level in the IO was 0.55 ± 0.13 ppm, while the serum Cu level in AO tended to be higher, namely 0.62 ± 0.19 ppm (p > 0.05). The IO had BCS of 1.56 ± 0.39, while the AO had a higher BCS of 2.50 ± 0.58 (p < 0.05). It could be concluded that ovarian inactivity in Bligon goats with poor body condition had a close association with microminerals Cu and Zn metabolism. It is necessary to pay attention to nutritional aspect of this animal to improve its reproductive and productive performance as well as health condition.
The impact of training chemotherapy safety standards with a smartphone application on the knowledge, attitude, and performance of nurses
Authors: Hojati Zohreh , Goudarzi Fateme , Hasanvand Shirin , Galehdar Nasrin , Birjandi Mehdi 
Published: November 2022

Abstract

Background: Nurses who play the leading role in caring for patients, especially nurses in the chemotherapy department who are constantly exposed to high-risk drugs and their side effects, should pay more attention to occupational safety. This study was performed to determine the effect of training chemotherapy safety standards using a smartphone application on nurses' knowledge, attitude, and practice.
Methods: The whole enumeration selected fifty oncology nurses from 3 hospitals affiliated with Lorestan University of Medical Sciences (western Iran). The study was conducted from June to November 2021. The training was done for four weeks with a smartphone application, including six main courses of familiarity with antineoplastic drugs, personal protective equipment, drug preparation, prescription and transfer, waste leakage, and elimination. The nurses' knowledge, attitude, and performance questionnaire were completed before, immediately, and one month after the intervention. Data analysis was performed using SPSS version 26, descriptive and inferential statistical tests of independent t-test, one-way analysis of variance, Spearman's rank correlation coefficient, repeated measures analysis of variance, and the Generalized Estimating Equation (GEE) model.
Results: Mean knowledge score of participants before, immediately, and one month after the intervention was (47.18 ± 8.19), (60.08 ± 3.82), and (61.88 ± 3.45), respectively. The mean attitude score of participants before, immediately, and one month after the intervention was (30.34 ± 3.94), (34.32 ± 3.25), and (34.98± 2.88), respectively, and the mean performance score of participants before, immediately, and one month after the intervention was (43.60 ±5.11), (51.78 ± 3.15) and (52.88 ± 3.06), respectively. The mean nurses' knowledge, attitude, and performance score increased significantly over time (P <0.001).
Conclusions: Teaching chemotherapy safety standards using the application improved oncology nurses' knowledge, attitude, and performance. Appropriate educational programs, especially new methods such as e-learning, are recommended for providing safety for nurses and methods of its promotion.
Engineered Myeloid Precursors Differentiate into Osteoclasts and Resorb Heterotopic Ossification in Mice
Authors: Rementer Cameron , Yavirach Apichai , Buranaphatthana Worakanya , Ausk Brandon , Speer Mei , Pierce Kate , Dharmarajan Subramanian , Scatena Marta , Leber Elizabeth , Rasch Alexander , Sangiorzan Bruce , Bain Steven , Giachelli Cecilia 
Published: November 2022

Abstract
Heterotopic ossification (HO) is prevalent following orthopedic trauma, traumatic brain and spinal cord injuries, brain, and limb amputations, particularly in combat veterans. HO causes pain, limited mobility and decreased quality of life. Current treatments are limited and have significant complications with high recurrence rates, underscoring the need for improved therapeutic interventions. In this study, we used genetically engineered osteoclasts (OCs) as a cell therapy to treat HO. Inducible, engineered myeloid precursors (iRANK cells) treated with chemical inducer of dimerization (CID) differentiated into TRAP+ multinucleated OCs, expressed functional osteopontin, and resorbed mineralized tissues in vitro. To investigate if iRANK OCs could reduce HO in vivo, HO lesions were induced by injections of BMP-2, and iRANK cells were locally delivered to ectopic bone nodules with concomitant systemic administration of CID. Micro-CT and histology showed that HO lesions were significantly reduced, and more OCs were observed in the cell-treated group compared to the control. Moreover, many OCs were TRAP, MMP9, and GFP-positive indicating that they differentiated from delivered iRANK cells. Thus, our data confirm the ability of engineered myeloid precursors to differentiate into OCs and resorb HO lesions in vivo paving the way for OC delivery as a promising approach for HO treatment.
Evaluation of large-scale medical equipment in public hospitals in China from the perspective of value-based medicine: a Delphi study
Authors: Ji-Zhuo Peng , WU Wei-Xuan , XU Jun , Ting-Ting Yang , ZHANG Zi-Cheng , Cheng-cheng LU , De-min Li 
Published: November 2022

Abstract
Objective
To select the value evaluation index system of large-scale medical equipment in public hospitals in China, to provide an evidence-based decision-making basis for the introduction and management of large-scale medical equipment.
Methods
Based on the theory of value medical and whole life cycle, literature analysis was used to establish the original indicator library of value evaluation of large-scale medical equipment. We used a 2-round Delphi technique to conduct expert consultation on the alternative value evaluation indicators of large-scale medical equipment, and further select indicators to build a value evaluation index system to determine its weight.
Results
A total of 17 experts were selected in this study to participate in the consultation, with an average professional period of 19.15. The positive coefficients of the two rounds of consultation were 88% and 100% respectively. The expert authority coefficient of the first round and the second round was 0.83. In the second round of consultation, the expert consistency coefficient (Kendall's W) of the first-level indicators and the second-level indicators were 0.305 and 0.387 (P < 0.005). The weights of engineering value, clinical value, economic value, and social value were 0.2433, 0.3267, 0.2433, and 0.1867, respectively. The Delphi coordinating group agreed that 24 secondary indicators were finally selected.
Conclusion
This study has built a large-scale medical equipment value evaluation index system through the Delphi method.The second round of consulting experts' evaluation opinions tend to be consistent, with a high degree of coordination. The constructed value evaluation index system of large-scale medical equipment is scientific and reliable.
Pollen morphology of the genus Gossypium and its systematic implications
Authors: Cai Xiaoyan , Hou Yuqing , Wang Heng , Xu Yanchao , Zheng Jie , Wang Yuhong , Liu Fang , Zhou Zhongli , Hua Jinping , Wang Kunbo 
Published: November 2022

Abstract
Background
Plants develop a variety of pollen morphological features during long-term evolution, which are controlled by genes and are not easily affected by the external natural environment. Therefore, pollen morphology has great significance in plant taxonomy, evolution and identification. However, there is no detailed study on the pollen morphology of the Gossypium genus although some cotton species have been investigated in the scattered reports. In this study, the pollen morphology of 23 cotton species was comprehensively examined using scanning electron microscopy to evaluate the pollen diversity of the genus and its taxonomic significance.
Results
The common characteristics of Gossypium pollen are spherical, radially symmetrical, echinate, panporate and operculate. The pollen diameter ranges from 62.43µm in G. harknessii to 103.41µm in G. barbadense, revealing that there are great variations in pollen size among cotton species. Moreover, the exine sculpture is echinate. The exine echini shape is mostly conical or sharply conical and occasionally rodlike. Echini density is found maximum in G. incanum (173) and minimum in G.gossypioides (54), meanwhile, echini length varies from 3.53 µm in G. herbaceum to 9.47 µm in G. barbadense. In addition, all cotton species are divided into three clusters based on cluster analysis, which provides new understanding of the evolution and phylogeny of the Gossypium genus.
Conclusion
Although the pollen characteristics alone are insufficient to reconstruct taxonomic and systematic relationships within the genus Gossypium, our findings can enrich our knowledge of sporopollen morphology and fill the phenological gap of these taxa and will contribute to future systematic and phylogenetic studies of the Gossypium genus.