bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene mapping methodology powered by induced genome rearrangements
Authors: Yone Hideyuki , Kono Hiromitsu , Hirai Hayato , Ohta Kunihiro 
Published: October 2022

AbstractPhenotypic variation occurs through genome rearrangements and mutations in certain responsible genes; however, systematic gene identification methodologies based on genome rearrangements have not been fully established. Here, we explored the loci responsible for the given phenotype using the TAQing system and compared it with a conventional mutagenesis-based method. Two yeast strains with different genetic backgrounds and flocculation phenotypes were fused and genomic rearrangements were induced by transient DNA breaks. Then, selection pressure was applied and multiple mutants were generated, showing different flocculation abilities. We also raised mutants with altered cohesiveness due to spontaneous mutations during long-term recursive passages of haploid strains without TAQing treatment. Comparative genomic analysis of the TAQed mutants revealed three chromosomal regions harboring pivotal flocculation genes, whereas conventional mutagenesis generated a more diverse list of candidate loci after prolonged selection. The combined use of these approaches will accelerate the identification of genes involved in complex phenotypes.
Potential link between high FIB-4 score and chronic kidney disease in metabolically healthy men
Authors: Kuma Akihiro , Mafune Kosuke , Uchino Bungo , Ochiai Yoko , Miyamoto Tetsu , Kato Akihiko 
Published: October 2022

AbstractAlthough the association between non-alcoholic fatty liver disease and chronic kidney disease (CKD) has been well known, it is unclear whether Fibrosis-4 (FIB-4) score is a predictor of CKD development. We performed this retrospective cohort study, with a longitudinal analysis of 5-year follow-up data from Japanese annual health check-ups. Participants with CKD (estimated glomerular filtration rate [eGFR] < 60 mL/min/1.73 m2 and/or proteinuria) and a habit of alcohol consumption were excluded. The cut-off FIB-4 score was 1.30, indicating increased risk of liver fibrosis. Overall, 5353 participants (men only) were analyzed without exclusion criteria. After propensity score matching, high FIB-4 score (≥ 1.30) was not an independent risk factor for incident CKD (odds ratio [OR] 1.57; 95% confidence interval [CI] 0.97–2.56). However, high FIB-4 score was a significant risk factor for CKD in non-obese (OR 1.92; 95% CI 1.09–3.40), non-hypertensive (OR 2.15; 95% CI 1.16–3.95), or non-smoking (OR 1.88; 95% CI 1.09–3.23) participants. In these participants, FIB-4 score was strongly associated with eGFR decline in the multiple linear regression analysis (β = − 2.8950, P = 0.011). Therefore, a high FIB-4 score may be significantly associated with CKD incidence after 5 years in metabolically healthy participants.
Investigating the rate of skeletal muscle atrophy in men and women in the intensive care unit: a prospective observational study
Authors: Wu Ruo-Yan , Sung Wei-Hung , Cheng Hui-Chen , Yeh Huan-Jui 
Published: October 2022

AbstractMuscle atrophy greatly affects the prognosis of patients in the intensive care unit, but the rate of change remains unclear. In this prospective observational study, we used ultrasound to measure the change in muscle thickness of the rectus femoris (RF) and vastus intermedius (VI) in 284 patients who were admitted to the SICU of Taoyuan General Hospital between January 1 and June 30, 2020. Patients were excluded if there is a wound at the right thigh which hinders the ultrasonography probe from placing. Daily rates of muscle atrophy were calculated using linear analysis and the ratios of change were plotted against the period of hospitalization. Patient characteristics were adjusted using propensity score matching and differences between men and women were analyzed. A linear mixed model was used to calculate the influence of other factors on muscle loss. The average daily atrophy rates of the RF and VI were 0.84% and 0.98%, respectively. The rate of atrophy was the highest in the third and fourth weeks. Daily atrophy rates of the RF and VI were approximately three times higher in women than in men. Protective factors of muscle atrophy included higher BMI and lower initial thickness of the RF and VI. Our study depicts the trend of muscle atrophy in the ICU and suggests more discussion in prevention to be conducted especially for women.
Targeted-sequence of normal urothelium and tumor of patients with non-muscle invasive bladder cancer
Authors: Hayashi Yujiro , Fujita Kazutoshi , Sakai Kazuko , Adomi Shogo , Banno Eri , Nojima Satoshi , Tomiyama Eisuke , Matsushita Makoto , Kato Taigo , Hatano Koji , Kawashima Atsunari , Minami Takafumi , Morii Eiichi , Uemura Hirotsugu , Nishio Kazuto , Nonomura Norio 
Published: October 2022

AbstractDuring tumorigenesis, certain tissues are colonized by mutant clones with oncogenic driver mutations as precancer lesions. These mutations can facilitate clonal expansion and may contribute to malignant transformation. The molecular features of low-grade non-muscle invasive bladder cancer (NMIBC) and high-grade bladder cancer are so distinct that they are thought to follow different evolutionary tumorigenesis pathways. Although NMIBC accounts for most bladder tumors, the somatic mutation patterns in “precancer” urothelium of patients with NMIBC remain unclear. Here, we analyzed specimens of normal urothelium and bladder tumors from patients with low-grade and high-grade NMIBC and investigated the genomic evolution of the cancer. Somatic mutations were analyzed using 50 oncogene-targeted sequences and droplet digital polymerase chain reaction for TERT promoter mutations. Somatic mutations in TERT promoter, FGFR3, and CDKN2A were characteristically identified in the normal urothelium of patients with NMIBC. These mutations, consistently identified in both tumor and normal specimens, likely affect clonal expansion during the malignant transformation of NMIBC. Though larger samples and comprehensive study are warranted to confirm our results, the difference in mutational landscape of the precancerous urothelium of patients with bladder cancer could offer deeper understandings of genomic evolution in bladder tumorigenesis.
Temporal link prediction via adjusted sigmoid function and 2-simplex structure
Authors: Zhang Ruizhi , Wang Qiaozi , Yang Qiming , Wei Wei 
Published: October 2022

AbstractTemporal network link prediction is an important task in the field of network science, and has a wide range of applications in practical scenarios. Revealing the evolutionary mechanism of the network is essential for link prediction, and how to effectively utilize the historical information for temporal links and efficiently extract the high-order patterns of network structure remains a vital challenge. To address these issues, in this paper, we propose a novel temporal link prediction model with adjusted sigmoid function and 2-simplex structure (TLPSS). The adjusted sigmoid decay mode takes the active, decay and stable states of edges into account, which properly fits the life cycle of information. Moreover, the latent matrix sequence is introduced, which is composed of simplex high-order structure, to enhance the performance of link prediction method since it is highly feasible in sparse network. Combining the life cycle of information and simplex high-order structure, the overall performance of TLPSS is achieved by satisfying the consistency of temporal and structural information in dynamic networks. Experimental results on six real-world datasets demonstrate the effectiveness of TLPSS, and our proposed model improves the performance of link prediction by an average of 15% compared to other baseline methods.
Inter-organellar and systemic responses to impaired mitochondrial matrix protein import in skeletal muscle
Authors: Neupane Nirajan , Rajendran Jayasimman , Kvist Jouni , Harjuhaahto Sandra , Hu Bowen , Kinnunen Veijo , Yang Yang , Nieminen Anni I. , Tyynismaa Henna 
Published: October 2022

AbstractEffective protein import from cytosol is critical for mitochondrial functions and metabolic regulation. We describe here the mammalian muscle-specific and systemic consequences to disrupted mitochondrial matrix protein import by targeted deletion of the mitochondrial HSP70 co-chaperone GRPEL1. Muscle-specific loss of GRPEL1 caused rapid muscle atrophy, accompanied by shut down of oxidative phosphorylation and mitochondrial fatty acid oxidation, and excessive triggering of proteotoxic stress responses. Transcriptome analysis identified new responders to mitochondrial protein import toxicity, such as the neurological disease-linked intermembrane space protein CHCHD10. Besides communication with ER and nucleus, we identified crosstalk of distressed mitochondria with peroxisomes, in particular the induction of peroxisomal Acyl-CoA oxidase 2 (ACOX2), which we propose as an ATF4-regulated peroxisomal marker of integrated stress response. Metabolic profiling indicated fatty acid enrichment in muscle, a shift in TCA cycle intermediates in serum and muscle, and dysregulated bile acids. Our results demonstrate the fundamental importance of GRPEL1 and provide a robust model for detecting mammalian inter-organellar and systemic responses to impaired mitochondrial matrix protein import and folding.
Youth with severe mental illness and complex non-somatic motor abnormalities: conflicting conceptualizations and unequal treatment
Authors: Andersson Peter , Wachtel Lee E. , Lundberg Johan , Jamshidi Esmail , Bring Johan , Rask-Andersen Mathias , Jarbin Håkan , Jokinen Jussi , Desai Boström Adrian E. 
Published: October 2022

AbstractTwo emerging diagnostic concepts promote distinct treatments for youth with acute-onset motor abnormalities and severe concurrent psychiatric symptoms: Pediatric acute-onset neuropsychiatric syndrome (PANS) and pediatric catatonia. Both have institutional approval in parts of Europe and in the USA, meriting an unconditional comparison of supporting evidence. Here we report results of qualitative and quantitative analyses of literature and Swedish National Registry Data suggesting that (1) catatonic patients are liable to fulfilling diagnostic criteria for PANS, (2) three conservatively assessed PANS case-reports present with possible unrecognized catatonia, (3) lithium and electroconvulsive therapy usage frequencies in Swedish minors (exclusively recommended for severe mental illness) are strongly intercorrelated and unequally distributed across Swedish counties, (4) established severe mental disorders are rarely overtly considered amongst PANS-specific research and (5) best-available evidence treatments appear markedly superior for pediatric catatonia compared to PANS in both childhood and adolescence. Prioritizing treatments for pediatric catatonia in concerned subjects could markedly improve treatment outcomes.
Establishing the accuracy of asteroseismic mass and radius estimates of giant stars
Authors: Brogaard K. , Arentoft T. , Slumstrup D. , Grundahl F. , Lund M. N. , Arndt L. , Grund S. , Rudrasingam J. , Theil A. , Christensen K. , Sejersen M. , Vorgod F. , Salmonsen L. , Ørtoft Endelt L. , Dainese S. , Frandsen S. , Miglio A. , Tayar J. , Huber D. 
Published: October 2022

Context. Eclipsing binary stars with an oscillating giant component allow accurate stellar parameters to be derived and asteroseismic methods to be tested and calibrated. To this aim, suitable systems need to be firstly identified and secondly measured precisely and accurately. KIC 4054905 is one such system, which has been identified, but with measurements of a relatively low precision and with some confusion regarding its parameters and evolutionary state.
Aims. Our aim is to provide a detailed and precise characterisation of the system and to test asteroseismic scaling relations.
Methods. Dynamical and asteroseismic parameters of KIC 4054905 were determined from Kepler time-series photometry and multi-epoch high-resolution spectra from FIES at the Nordic Optical Telescope.
Results. KIC 4054905 was found to belong to the thick disk and consist of two lower red giant branch (RGB) components with nearly identical masses of 0.95 M⊙ and an age of 9.9 ± 0.6 Gyr. The most evolved star with R ≃ 8.4 R⊙ displays solar-like oscillations. These oscillations suggest that the star belongs to the RGB, supported also by the radius, which is significantly smaller than the red clump phase for this mass and metallicity. Masses and radii from corrected asteroseismic scaling relations can be brought into full agreement with the dynamical values if the RGB phase is assumed, but a best scaling method could not be identified.
Conclusions. The dynamical masses and radii were measured with a precision better than 1.0%. We firmly establish the evolutionary nature of the system to be that of two early RGB stars with an age close to 10 Gyr, unlike previous findings. The metallicity and Galactic velocity suggest that the system belongs to the thick disk of the Milky Way. We investigate the agreement between dynamical and asteroseismic parameters for KIC 4054905 measured in various ways. This suggests that consistent solutions exist, but the need to analyse more of these systems continues in order to establish the accuracy of asteroseismic methods.
LHAASO J2226+6057 as a pulsar wind nebula
Authors: De Sarkar Agnibha , Zhang Wei , Martín Jonatan , Torres Diego F. , Li Jian , Hou Xian 
Published: October 2022

Context. The Large High Altitude Air Shower Observatory has reported the detection of cosmic-ray sources in the Milky Way that can accelerate particles up to PeV (=1015 eV) energies. These sources, so-called “PeVatrons”, are mostly unidentified. Several classes of sources, such as supernova remnants, pulsar wind nebula, or young stellar clusters can potentially be the counterparts of these PeVatrons.
Aims. The aim of this work is to study a pulsar wind nebula interpretation of one of these PeVatrons, LHAASO J2226+6057, which has a relatively well covered multifrequency spectrum.
Methods. We have performed a leptonic, time-dependent modeling of the pulsar wind nebula (PWN) associated with PSR J2229+6114 considering a time-energy-dependent diffusion-loss equation. Injection, energy losses, as well as the escape of particles were considered to balance the time-dependent lepton population. We have also included the dynamics of the PWN and the associated supernova remnant and their interaction via the reverse shock to study the reverberation phase of the system.
Results. We have considered different values of the braking index (n) and true age (tage) for the fitting of the MultiWaveLength (MWL) spectral energy distribution (SED) of LHAASO J2226+6057. The best-fit PWN model parameters and their 1σ confidence intervals have been evaluated. We have also demonstrated the impact of reverberation on the MWL SED with increasing time. Additionally, we have discussed the resultant large radius and low magnetic field associated with the PWN in question, as caveats for the possible physical connection of the pulsar as the origin of this high energy source.
Isolated toughness variant and fractional κ-factor
Authors: He Zhengyue , Liang Li , Gao Wei 
Published: October 2022

Isolated toughness is a crucial parameter considered in network security which characterizes the vulnerability of the network from the perspective of graph topology. I’(G) is the unique variant of isolated toughness which was introduced in 2001. This work investigates the correlation of I’(G) and the existence of fractional factor. It is proved that a graph G with δ(G) ≥ k admits fraction k-factor if I’(G) > 2k − 1, where k ≥ 2 is an integer. A counterexample is presented to show the sharpness of I’(G) bound.
Echokardiografische Graduierung der Mitralklappen- und der Trikuspidalklappeninsuffizienz
Authors: Schneider Matthias , Binder Thomas 
Published: October 2022

ZusammenfassungDie Mitralklappeninsuffizienz und Trikuspidalklappeninsuffizienz sind häufige Vitien. Die Echokardiografie ist essenziell bei der initialen Diagnose und Graduierung, während therapeutischer
Interventionen sowie in den Verlaufskontrollen. Sowohl für die Mitralklappe als auch für die Trikuspidalklappe gilt das gleiche Grundprinzip: Zunächst muss der Mechanismus der Insuffizienz
erkannt werden, es geht um die Unterscheidung einer primären/strukturellen von einer sekundären/funktionellen Insuffizienz. Im Anschluss erfolgt eine holistische Analyse des Schweregrads der
Insuffizienz, die immer qualitative, quantitative sowie semiquantitative Aspekte umfasst.
Dialektisch-Behaviorale Therapie bei
Substanzgebrauchsstörungen: DBT-S
Authors: Förster Julia 
Published: October 2022

ZusammenfassungDie Dialektisch Behaviorale Therapie (DBT) wurde für Menschen mit
schwerwiegenden Problemen der Emotionsregulation und der Impulskontrolle
entwickelt. Substanzkonsum ist eine häufige Folge dieser Probleme. Die
Betroffenen sollen im Rahmen der Behandlung neue Verhaltensfertigkeiten
erlernen, um dadurch in der Lage zu sein ihr Problemverhalten zu reduzieren und
lösungsorientierte Strategien einzusetzen, so dass für sie ein
sinnerfülltes Leben möglich ist. Im folgenden Beitrag wird die
Dialektisch-Behaviorale Therapie bei Borderline- und
Subtanzgebrauchsstörung (DBT-S) als strukturierte Behandlungsmethode
beschrieben.
Phyllobilins – Bioactive Natural Products Derived from Chlorophyll – Plant Origins, Structures, Absorption Spectra, and Biomedical Properties
Authors: Karg Cornelia A. , Taniguchi Masahiko , Lindsey Jonathan S. , Moser Simone 
Published: October 2022

AbstractPhyllobilins are open-chain products of the biological degradation of chlorophyll a in higher plants. Recent studies reveal that phyllobilins exert anti-oxidative and
anti-inflammatory properties, as well as activities against cancer cells, that contribute to the human health benefits of numerous plants. In general, phyllobilins have been overlooked in
phytochemical analyses, and – more importantly – in the analyses of medicinal plant extracts. Nevertheless, over the past three decades, > 70 phyllobilins have been identified upon
examination of more than 30 plant species. Eight distinct chromophoric classes of phyllobilins are known: phyllolumibilins (PluBs), phylloleucobilins (PleBs), phylloxanthobilins (PxBs), and
phylloroseobilins (PrBs)–each in type-I or type-II groups. Here, we present a database of absorption and fluorescence spectra that has been compiled of 73 phyllobilins to facilitate
identification in phytochemical analyses. The spectra are provided in digital form and can be viewed and downloaded at www.photochemcad.com. The present review describes the plant origin,
molecular structure, and absorption and fluorescence features of the 73 phyllobilins, along with an overview of key medicinal properties. The review should provide an enabling tool for the
community for the straightforward identification of phyllobilins in plant extracts, and the foundation for deeper understanding of these ubiquitous but underexamined plant-derived
micronutrients for human health.
Clarification of the influences of superficial velocity U
g and particle diameter d
p on the gas–solid flow characterizations in a rolling circulating fluidized bed (RCFB) by electrical capacitance tomography (ECT) method
Authors: Wang Zhilong , Zhao Tong , Liu Xiayi , Zhou Chunguo , Zhang Yanchao , Murata Hiroyuki , Takei Masahiro 
Published: October 2022

The influences of the superficial velocity and particle diameter on gas–solid flow characterizations in a rolling circulation fluidized bed (RCFB) are clarified by using the electrical capacitance tomography method. Gas–solid flow characterizations include the time-space averaged particle volume fraction ᾱz′, the time transition of the cross-sectional average particle volume fraction 〈α〉t, and the radial profile of the particle volume fraction 〈α〉r/R. Two kinds of particles are selected in the case that the particle diameter is 0.22 mm and the superficial velocity is set to 2.0, 3.0, and 4.0 m/s and in the case that the particle diameter is 0.45 mm and the superficial velocity is set to 3.0, 4.0, and 5.0 m/s. After analyzing the results, the main conclusions are able to be summarized as follows: First, when compared with the superficial velocity, ᾱz′ visualized from particle distribution images is much more influenced by the particle diameter. Second, the fluctuations of 〈α〉t and 〈α〉r/R tend to be more serious under the conditions of larger particle and lower riser’s height, which are able to be quantitatively explained by the fast Fourier transform of 〈α〉t and the spatial standard deviation 〈σs〉 of 〈α〉r/R. Consequently, a strategy to guarantee both particle distribution stability and particle distribution uniformity within the RCFB is proposed.
The syntax of progressive and ingressiveaanhet-constructions in Dutch
Authors: Bogaards Maarten , Boogaart Ronny , Barbiers Sjef 
Published: October 2022

AbstractThis paper presents a novel syntactic analysis of the much-debated Dutchaanhet-construction, e.g.Pieter is aan het opruimen‘lit. Peter is on the cleanup: Peter is cleaning up’. We show that the construction’s syntactic behavior varies with the matrix verb: progressivezijn‘be’ versus ingressivegaan‘go’ andslaan‘hit’. Based on this variation, we argue that there are twoaanhet-projections occupying different synchronic positions on a functional-to-lexical cline.
Variation in the processing of grammatical norm violations
Authors: Cos Floris , Hubers Ferdy 
Published: October 2022

Abstract
So far, processing studies on grammatical norm violations (GNVs) in Dutch (i.e. als ‘as’ in
comparatives) have mainly focused on general differences between GNVs and their grammatical and ungrammatical counterparts. The
present study is the first to also systematically investigate between-participant and between-construction variation in the
processing of GNVs, using a self-paced reading task. Age and educational level were investigated as potential sources of
between-participant variation, and between-construction variation was assessed by including three GNVs that vary in the amount of
prescriptive attention they receive in society. Results indeed showed that the processing of GNVs was influenced by the age and
educational level of participants. Moreover, different results were obtained for different norm violations. Based on these
results, we conclude that it is very important to take into account differences between participants and constructions when
studying the processing of GNVs.
Pronouns of address in recruitment advertisements from multinational companies
Authors: den Hartog Maria , van Hoften Marjolein , Schoenmakers Gert-Jan 
Published: October 2022

Abstract
In Netherlandish Dutch, Belgian Dutch, German, French, and Spanish, speakers have a choice between formal (V) and informal (T) pronouns of address. We present a quantitative study of how V and T are used on recruitment pages of multinational companies. Our corpus-based method is inspired by studies on pronouns of address in Netherlandish and Belgian Dutch by Vismans (2007) and Waterlot (2014). Unlike these earlier studies, we provide a comparison of the same companies recruiting in different countries, thereby strengthening the comparison of V- and T-forms between languages. We find a preference for T in recruitment ads in Belgian Dutch, Netherlandish Dutch, and Spanish, while we find a preference for V in French. There seems to be no clear preference for either V or T in German, which may reflect that address preferences in German are changing or ambiguous.