bluuebunny/crossref_metadata_2025_split huggingface dataset:
High GTSE1 expression promotes cell proliferation, metastasis and cisplatin resistance in ccRCC and is associated with immune infiltrates and poor prognosis
Authors: Lei Pu , Wang Ziming 
Published: July 2022

Abstract
Background: Clear cell renal cell carcinoma (ccRCC) is the most common and fatal form of kidney cancer, accounting for 80% of new cases. Although it has been reported that GTSE1 is highly expressed in a variety of tumors and associated with malignant progression and poor clinical prognosis, its clinical significance, correlations with immune infiltration and biological function in ccRCC are still poorly understood.Methods: The gene expression, clinicopathological features, and clinical significance of GTSE1 were analyzed by multiple databases, including TCGA, GEO, TIMER, and ﻿UALCAN. Kaplan–Meier survival analysis; ﻿Gene set enrichment analysis (GSEA); ﻿Gene ontology enrichment (GO);Kyoto Encyclopedia of Genes and Genomes (KEGG); Tumor-infiltrating immune cells and immunomodulators were extracted and analyzed using ﻿TCGA ccRCC ﻿profiles. Protein-Protein interactions were built using the STRING website. The protein level of GTSE1 in ccRCC patients was detected by ﻿immunohistochemistry assay using the ccRCC tissue chip. Finally, MTT assay, colony formation assay, ﻿cell flow cytometry analysis, EdU staining assay, wound healing assay, transwell migration, and invasion assay were conducted to assess the biological function of GTSE1 in vitro. ﻿Results: GTSE1was overexpressed in ccRCC tissues and cells, and the overexpression of GTSE1 was associated with adverse clinical-pathological factors and poor clinical prognosis. Meanwhile, the functional enrichment analysis indicated that GTSE1 and its co-expressed genes were mainly related to the cell cycle, DNA replication, and immunoreaction such as T cell activation and innate immune response through multiple signaling pathways including the P53 signaling pathway and T cell receptor signaling pathway. Furthermore, we observed a significant relationship between the GTSE1 expression and immune infiltrating levels of different immune cells in ccRCC. Biological functional studies also demonstrated that GTSE1 could promote malignant progression of ccRCC by promoting cell proliferation, cell cycle transition, migration, invasion capacity, and decreasing the sensitivity of ccRCC cells to cisplatin. Conclusion: Our results indicate that GTSE1 serving as a potential oncogene that can promote the malignant progression and cisplatin resistance in ccRCC. Additionally, High GTSE1 expression contributes to an increased level of immune cell infiltration and is associated with a worse prognosis, providing a potential target for tumor therapy in ccRCC.
Gelatinous zooplankton versus Algerian environmental factors
Authors: KHAMES Ghollame Ellah Yacine , ALIOUA Zakia , KHERCHOUCHE Aldjia , HAFFERSSAS Aziz 
Published: July 2022

Abstract
Factors governing gelatinous zooplankton distributions and dispersal have long interested pelagic ecologists. The objective of the present study was to investigate the distribution of gelatinous zooplankton in relation to environmental parameters of coastal Algeria (Southwestern Mediterranean Sea). The zooplankton samples were collected on nine stations located in the central (Sidi Fredj) and western (Habibas Islands) sectors of the Algerian coast during 2012 and 2013. A total of 48 species belonging to 4 groups: Cnidaria, Tunicata, Chaetognathae and Mollusca have been recorded. Their composition showed a variable distribution in both investigated zones according to ANOSIM (p < 0.05) whatever the sampling period and between May and July for Habibas Islands and confirmed by NMDS, but no differences were detected between the sampling periods for Sidi Fredj. The redundancy analysis was used to identify different sample assemblages. It showed relationships between temperature, salinity and chlorophyll a with zooplankton abundances. Temperature was found to be the strongest explanatory variable for some Cnidaria (e.g. Aglaura hemistoma, Rhopalonema velatum, Liriope tetraphylla and Pelagia noctiluca) and Appendicularia (e.g. Oikopleura longicauda, Oikopleura fusiformis and Fritillaria pellucida) while, the development of Doliolum nationalis and Creseis virgula were related to salinity variations.
Neutrophil-lymphocyte Ratio is a Negative Prognostic Biomarker for Luminal A Breast Cancer
Authors: Xiang Yimei , Zhang Ningning , Lei Haike , Wu Jing , Wang Wenting , Zhang Huan , Zeng Xiaohua 
Published: July 2022

Abstract
Background
Inflammation plays an important role in the occurrence, development and metastasis of tumors. The neutrophil-lymphocyte ratio (NLR) is widely used to evaluate global inflammation in various tumor types. However, the prognostic role of NLR in patients with Luminal A breast cancer is rarely reported. The purpose of this study was to investigate the relationship between preoperative NLR and the survival rate of patients with Luminal type A.
Methods
This study collected the data of 226 Luminal A breast cancer patients who were eligible in the Chongqing University Cancer Hospital from January 2011 to December 2016, and analyzed the relationship between preoperative NLR and survival time. The cutoff value of NLR was defined by ROC curve, and the overall survival (OS) time was estimated by Kaplan-Meier method. Cox analysis was performed to determine the correlation between clinicopathological parameters and prognosis.
Results
The OS of patients with NLR lower than 2.0 was significantly longer than that of patients with higher NLR (P < 0.0001). In the univariable cox regression analysis, the NLR, T stage (T3-T4 vs. T1-T2) and histology grade (II-III vs. I) were all significantly associated with OS. In the multivariable cox regression analysis, the NLR and the histology grade (II-III vs. I) were independent factors, the hazard ratio was 36.003 (95% CI: 4.912–263.92, P < 0.0001) and 1.924 (95% CI: 1.14–3.25, p = 0.014) respectively.
Conclusions
The results suggest that higher preoperative NLR related to worse prognosis in Luminal A breast cancer, NLR has a high prognostic value for patients with Luminal A breast cancer and preoperative NLR can be used as a reference index to evaluate the prognosis of Luminal A breast cancer.
Photoinhibiting via simultaneous photoabsorption and free-radical reaction for high-fidelity light-based bioprinting
Authors: He Ning , Wang Xiaonan , Shi Liyang , Li Jing , Mo Lan , Chen Feng , Huang Yuting , Liu Hairong , Zhu Xiaolong , Zhu Wei , Mao Yiqi , Han Xiaoxiao 
Published: July 2022

Abstract
Light-based three-dimensional (3D) bioprinting is now employed widely to fabricate geometrically complex constructs for various biomedical applications. However, the inherent light scattering defect creates significant challenges in patterning dilute hydrogels to form high-fidelity structures with fine-scale features. Herein, we introduce an innovative photoinhibiting approach that can suppress the light scattering effect more effectively than the current purely photoabsorbing-based method via a new mechanism of simultaneous photoabsorption and free-radical reaction. Mechanistically, photoabsorption can confine vertical light penetration depth by absorbing excess light, whilst the free-radical reaction can rapidly consume the free radicals generated by scattered light. We have showcased that this biocompatible approach significantly improves the printing resolution (~ 30 µm) and fidelity (geometric error less than 5%), while minimising the costly trial-and-error procedures simultaneously. The strategy established in this study promotes the printability and operability of light-based 3D bioprinting systems by addressing the light scattering issue, allowing for more advanced tissue engineering and regenerative medicine.
Diversity of RNA Editing in Chloroplast Transcripts across Three Main Plant Clades
Authors: Zhang Aidi , Fang Jing , Zhang Xiujun 
Published: July 2022

Abstract
RNA editing is a post-transcriptional modification of an RNA nucleotide sequence. Until now, different RNA editing systems were found in the major eukaryotic clades. In the plant kingdom, RNA editing was mainly documented in the mitochondria and chloroplast genomes. However, variation among large taxonomic groups and the evolutionary trajectory in terms of intra- and inter- clades remains unclear. To gain a better understanding of RNA editing evolution, in this study, based on publicly available RNA-seq data across three clades (fern, gymnosperm, and angiosperm), we provided a detailed analysis of chloroplast RNA editing events and discussed its evolution in land plants. A total of 5,205 editing sites were determined across 21 species after rigorous screening. We found that the clustering relations of RNA editing sites across 21 species agreed with the phylogenetic tree based on protein sequences approximately, and more editing sites occurred in early-diverging lineages for all the three clades, implying they shared similar evolutionary trajectories of editing loss. We observed that the average RNA editing level varied among species as well as genes, a lowest RNA editing level (~0.42) was detected in Selaginella moellendorffii; the highest editing level (~0.88) was detected in atpA gene. The reduction of cytosine content with evolution detected in our study further suggested that the substitution of the genomic sequence was the significant driver of loss of editing for later-branching plants. Many of the identified sites in our study have not been previously reported and provided a valuable data set for the future research community. Our findings also provide valuable information for the evolution of RNA editing in plants.
Current Management of the Urachal Anomalies (Ua). Lessons Learned From the Clinical Practice
Authors: Perez Dolev , Neeman Binyamin , Kocherov Stanislav , Jaber Gaudat , Armon Yaron , Zilber Sofia , Chertin Boris 
Published: July 2022

Abstract
Purpose: It has been suggested that symptomatic UA requires surgical excision. However, the management of asymptomatic urachus is still controversial. We aimed to evaluate the clinical presentation, the efficacy of current modalities used, and postoperative pathology in patients with UA. Material and Methods: We have performed a retrospective review of all patients diagnosed with UA and treated surgically or conservatively over 18 years. Demographic data, clinical presentation, imaging modalities, pathology, treatment, and postoperative complications were analyzed. Results: Twenty-five symptomatic patients (18 males and seven females) with a median age of 13 years (one month to 37 years) were identified. 15 (60%) were diagnosed with a urachal cyst, 4(16%) with sinus, 3(12%) with urachal diverticulum, and the remaining 3(12%) with patent urachus. Of those, 20(80%) underwent surgical repair, and the remaining five (20%) patients were managed conservatively. 4 (20%) underwent laparotomy, 7 (35%) laparoscopic incision, and the remaining 9 (45%) laparoscopic robotic-assisted surgery. Nine patients required bladder cuff excision. The median operative time was 75 minutes (42-140 min). One patient developed Clavien Dindo grade IIIA complication resulting in infected hematoma, which resolved after drainage. Another patient with a complication of grade IIIB needed reoperation as a result of recurrent events of an abscess. 13 (65%) demonstrated epithelium lining of the urachus on postoperative pathology.Conclusions: Our data show that most of the patients with UA presented with epithelial lining, which might lead to the later malignant transformation. It might cause a shift from the conservative management of asymptomatic patients to surgical intervention. Robotic-assisted surgery appears beneficial in these patients, especially when the bladder cuff excision is required.
Fibration structure for Gromov h-principle
Authors: Yamazaki Koji 
Published: July 2022

Abstract
The h-principle is a powerful tool for obtaining solutions to partial differential inequalities and partial differential equations. Gromov discovered the h-principle for the general partial differential relations to generalize the results of Hirsch and Smale. In his book, Gromov generalizes his theorem and discusses the sheaf theoretic h-principle, in which an object called a flexible sheaf plays an important role. We show that a flexible sheaf can be interpreted as a fibrant object with respect to a model structure. The sheaf of formal sections is interpreted as a fibrant replacement in the model structure. There are two main results. The first result is that the category of continuous sheaves can embed in a model category. The class of cofibrations is not included in the category of continuous sheaves. However, the category of continuous sheaves has weak equivalences and fibrations. The second result is that the category of continuous sheaves has an ABC prefibration structure, a generalized structure of a model structure.
3D Quantitative Measurement of the Coronoid Process and Proximal Ulna: A Chinese Adults-Based Morphometric Study
Authors: Wang Daofeng , Li Jiantao , Xu Gaoxiang , Zhang Hao , Xu Cheng , Zhang Wupeng , Li Hua , Gan Xuewen , Xiong Ying , Zhang Licheng , Li Li , Tang Peifu 
Published: July 2022

Abstract
Purpose To perform quantitative measurements of anatomic morphology of the proximal ulna and coronoid process and establish the morphologic references based on Chinese for the surgical protocol and implant design. Methods The computed tomography data of 156 upper extremities were involved in this study. The ulna model was reconstructed in Mimics. Ten distance and 6 angle parameters were measured by 4 independent investigators with a new 3D quantitative measurement method. The intraclass correlation coefficient was used to evaluate the measuring reliability. Gender and side differences of measured parameters were evaluated. Results Measurements showed a mean coronoid height of 15 mm, which was 42% of ulnar height with gender-specific differences (mean, 16 mm in men and 14 mm in women, p<0.001). A mean unsupported anteromedial facet width of 8 mm was 61% of coronoid anteromedial facet. A larger opening angle correlates to a larger olecranon-diaphysis angle (p<0.001) and larger coronoid height (p=0.001). A mean proximal ulna dorsal angulation of 4.7° is present in 80% of models at an average of 52 mm distal to olecranon tip. The average proximal ulna varus angulation was 16° at a mean of 74 mm distal to olecranon tip. Morphological features between the left and right sides were highly consistent. The high correlation between coronoid anatomic parameters, poor correlation among proximal ulnar parameters, and poor relevance between both was also found. The ICC was between 0.789 and 0.978 for inter-observer and between 0.696 and 0.997 for intra-observer reliability.Conclusions The proximal ulna features variable morphology and poor correlation with coronoid. Over half of the anteromedial facet was not supported by the proximal ulnar diaphysis, making the coronoid vulnerable to elbow trauma. Surgeons should pay more attention to the effect of gender difference and sides consistency of proximal ulna morphology on the treatment of fractures.
Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE)
Authors: Yun Yang , Wang Xuejiao , Xu Jingyi , Jin Chenye , Chen Jingyu , Wang Xueru , Wang Jianing , Qin Ling , Yang Pingting 
Published: July 2022

Abstract
Background
Pristane induced lupus (PIL) model is a useful tool to study environmental-related systemic lupus erythematosus (SLE). However, the involvement of neuropsychiatric manifestations in this model has not been investigated in detail. Since neuropsychiatric lupus (NPSLE) is an important complication of SLE, we investigated how PIL mice develop neuropsychiatric symptoms to explore its suitability for NPSLE study.
Results
We found that PIL mice showed olfactory dysfunction accompanied with an anxiety- and depression-like phenotype at month 2 or 4 after pristane injection. The levels of cytokines (IL-1β, IL-6, TNF-α and IL-10) in the brain and BBB permeability increased significantly from month 1, and persisted throughout the observed course of the disease. Then, IgG deposition in the choroid plexus and lateral ventricle wall were observed at month 1. Both astrocytes and microglia were activated at month 1. The activation of astrocytes was persistent throughout the observed course of the disease, while the microglia activation decayed dramatically at month 4. Lipofuscin deposition, a sign of neuronal damage, was detected in cortical and hippocampal neurons from month 4 to 8.
Conclusion
PIL mice showed a series of behavioral deficits and pathological changes in the brain, which might be applicable to investigate the development of pathogenesis and potential therapeutic targets of environmental-related NPSLE.
Classification prediction of early pulmonary nodes based on weighted gene correlation network analysis and machine learning
Authors: Li Guang , Yang Meng , Ran Longke , Jin Fu 
Published: July 2022

Abstract
Objective: To use weighted gene correlation network analysis (WGCNA) and machine learning algorithm to predict classification of early pulmonary nodes with public databases.Methods: The expression data and clinical data of lung cancer patients were firstly extracted from public database (GTEx and TCGA) to study the differentially expressed genes (DEGs) of lung adenocarcinoma (LUAD). The intersection of three R packages（Dseq2,Limma,EdgeR) methods were selected as candidate DEGs for further study. WGCNA was used to obtain relevant modules and key genes of lung cancer classification, GO and KEGG enrichment analysis was performed. The model was built using two machine learning methods, Least Absolute Shrinkage and Selection Operator (LASSO) regression and tumor classification was also predicted with extreme Gradient Boosting (XGBoost) algorithm. Results: DEGs analysis revealed that there were 1306 LUAD genes. WGCNA module analysis showed that a total of 116 genes were significantly related to classification, and module genes were mainly related to 14 KEGG pathways. The machine learning algorithm identified 10 target genes by LASSO regression analysis of differential genes, and 18 genes were identified by XGBoost model. A total of 6 genes were found from the intersection of the above methods as classification signatures of early pulmonary nodules， including "HMGB3" "ARHGAP6" "TCF21" "FCN3" "COL6A6" "GOLM1".Conclusion: Using DEGs analysis, WGCNA method and machine learning algorithm, 6 gene signatures related to early stage of LUAD , which can assist clinicians in disease classification prediction.
The role of social circle COVID-19 illness and vaccination experiences in COVID-19 vaccination decisions: a representative online survey of the United States population
Authors: Skidmore Mark 
Published: July 2022

Abstract
Background: Around the world, policymakers have clearly communicated that COVID-19 vaccination programs need to be accepted by a large proportion of the population to allow life return to normal. However, according to the Center for Disease Control, about one third of the United States population is still not fully vaccinated as of June 2022.Aims: The primary aim of this work is to identify the factors associated by American citizens with the decision to be vaccinated against COVID-19. In addition, the proportion of fatal events from COVID-19 vaccinations was estimated and compared with the data in the VAERS database.Methods: A nationally representative online survey of COVID-19 health experiences was conducted. Information was collected regarding reasons for and against COVID-19 inoculations, experiences with COVID-19 illness and COVID-19 inoculations by survey respondents and their social circles. Logit regression analyses were carried out to identify factors influencing the likelihood of being vaccinated.Results: A total of 2,840 participants completed the survey between December 18 and 23, 2021. Fifty-one percent of the participants were female and the mean age was 47 (95% CI: 46.36 – 47.64) years. Those who knew someone who experienced a health problem from COVID-19 were 6.5% (95% CI: 2.1 – 10.9) more likely to be vaccinated, while those who knew someone who reported a health problem following vaccination were 13.3% (95% CI: 8.4 – 18.2) less likely to be vaccinated. Thirty-four percent reported that they knew at least one person who had experienced a significant health problem due to the COVID-19 illness. Similarly, 22% of respondents indicated that they knew at least one person who had experienced a severe health problem following COVID-19 vaccination. With these survey data, the total number of fatalities due to COVID-19 inoculation may be as high as 278,000 (95% CI: 217,330—332,608) when fatalities that may have occurred regardless of inoculation are removed.Conclusions: Knowing someone who reported serious health issues either from COVID-19 or from COVID-19 vaccination are important factors for the decision to get vaccinated. The large difference in the possible number of fatalities due to COVID-19 vaccination that emerges from this survey and the available governmental data should be further investigated.
Initiation of anticoagulation therapy within 48-hour after esophageal variceal band ligation is safe in portal vein thrombosis patients having cirrhosis and acute variceal bleeding: A multi-centric randomized controlled trial
Authors: Gao Zhanjuan , Li Shanshan , Zhao Jingrun , Li Jinhou , Gao yanjing 
Published: July 2022

Abstract
Background and aims: Portal vein thrombosis (PVT) and acute variceal bleeding (AVB) are recurrent complications of cirrhosis. Safety, efficacy, and initiation time of anticoagulant treatment among cirrhotic patients having PVT and AVB are controversial issues. We aimed to establish the safety and efficacy of nadroparin calcium warfarin sequential (NWS) anticoagulation therapy within 48-hour after esophageal variceal band ligation within PVT patients having cirrhosis and AVB.Methods: Cirrhotic patients having AVB and PVT who underwent EVL were included and randomly allocated to either the NWS therapy group (one-month nadroparin calcium by subcutaneous injection following five-month warfarin through oral administration) or the control group (without any anticoagulation therapy). Five-day failure, 14-day, four-weeks, and six-month rebleeding rates after EVL, other major bleeding events in specific sites, and recanalization rate of PVT among the two groups were assessed at six months. Results: 118 patients were eligible and randomly divided into the NWS group and the control group, with 59 patients in either group. No patients experienced five-day failure in both groups. There was no significant difference in the rate of 14-day (0 vs. 1.7%, P = 1.000), four-week (0 vs. 3.4%, P = 0.476), and six-month rebleeding (11.9% vs. 18.6%, P = 0.36) between the two groups. The overall recanalization (complete and partial) rate in the NWS therapy group was significantly higher than in the control group (67.8% vs. 40.7%, P = 0.003). Low Child-Pugh score (P = 0.005), D-dimer<2.00 ug/mL (P = 0.016), and NWS anticoagulation therapy (P = 0.004) were the predictors of PVT recanalization through univariate analysis of binary logistic regression. NWS anticoagulation therapy (P = 0.004) was the independent factor of recanalization through multivariate analysis. In the NWS group, the Child-Pugh score (6.77 ±1.36 vs. 6.00 ±1.00, P = 0.001) and the albumin level (35.01 ±5.95 vs. 36.95 ±4.27, P = 0.048) were significantly improved at the sixth month.Conclusions: Initiation of NWS anticoagulation therapy within 48-hour after EVL is safe with better efficacy against PVT patients having cirrhosis and AVB. Moreover, it can also improve liver function.