bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR‐188‐3p/GRHL2 axis
Authors: Zhou Meixi , Yu Xiang , Li Chenping , Lou Lejing , Yang Song , Cai Jihao , Cai Chang 
Published: November 2022

AbstractProblemPreeclampsia (PE) is the main factor threatening the life of primipara. Defective migration and invasion of trophoblast cells was one of the causes of PE. Circ_0111277 had been reported to be related to the development of PE, but the mechanism of its effect on trophoblast cells needed further study.Method of StudyThe expression of circ_0111277, microRNA‐188‐3p (miR‐188‐3p) and grainyhead‐like 2 (GRHL2) mRNA were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR). Cell Counting Kit‐8 (CCK‐8) assay, 5‐Ethynyl‐20‐deoxyuridine (EdU) and colony formation assay were used to examine cell proliferation ability. Tube formation and transwell assay were performed to assess the angiogenesis and metastasis ability of cells. Western blot was applied to measure the levels of epithelial‐mesenchymal transition (EMT)‐related proteins (E‐cadherin and Vimentin) and GRHL2 protein. The relationship between miR‐188‐3p and circ_0111277 or GRHL2 was verified by the dual luciferase reporter experiment.ResultsCirc_0111277 and GRHL2 were elevated, and miR‐188‐3p was declined in PE patients. Overexpression of circ_0111277 could inhibit the proliferation, angiogenesis, migration, invasion and EMT of trophoblast cells (HTR‐8/Svneo). Circ_0111277 was the molecular sponge of miR‐188‐3p. MiR‐188‐3p up‐regulation could reduce the inhibition of HTR‐8/Svneo cell growth caused by overexpression of circ_0111277. GRHL2 was a target gene of miR‐188‐3p, and GRHL2 silencing relieved the adverse effects of miR‐188‐3p inhibitors on HTR‐8/Svneo. In general, circ_0111277 up‐regulated GRHL2 expression through sponge miR‐188‐3p.ConclusionHighly expressed circ_0111277 up‐regulated the expression of GRHL2 through sponge miR‐188‐3p, thereby inhibiting trophoblast cells function, which suggested a new molecular mechanism for the pathogenesis of PE.
Long and Short‐term Investments by European Banks – Trends Since the IASB Published IFRS 9
Authors: Löw Edgar , Erkelenz Marc 
Published: November 2022

AbstractIFRS 9 was introduced by the IASB in 2014 with a mandatory application in 2018. Three years later the IASB started a post‐implementation review project. Major concerns at a political level and from the (insurance) industry were raised about long‐term investment strategies that appear less favourable due to new measurement categories and impairment rules. Furthermore, it was expected that the new rules on categorisation would create more profit or loss (P/L) volatility. We show that concerns about banks' decreasing maturity investment behaviour and increased profit or loss volatility since the publication of IFRS 9 in 2014 could not be verified until 2020. Additionally, banks hardly use the fair value option in practice, which suggests it can be abolished. Finally, the paper finds that the fair value through other comprehensive income option for equity instruments has almost no importance for banks. The IASB should, therefore, abolish this category as well. Hence, the measurement categories could be reduced by two categories reducing complexity and increasing the decision usefulness of financial instruments accounting rules for investors.
Barriers to access adequate maternal care in Romania, Bulgaria, and Moldova: A cross‐country comparison
Authors: Miteniece Elina , Pavlova Milena , Rechel Bernd , Kabakchieva Margarita , Zuza Irina , Radu Ilinca , Groot Wim 
Published: November 2022

AbstractBackgroundEastern European health system indicators (e.g., number of health workers and care coverage) suggest well‐resourced maternity care systems, but maternal health outcomes compare poorly with those in Western Europe. Often, poor maternal health outcomes are linked to inequities in accessing adequate maternal care. This study investigates access‐related barriers (availability, appropriateness, affordability, approachability, and acceptability) to maternity care in Romania, Bulgaria, and Moldova.MethodsThis cross‐country study (n = 7345) is based on an online survey where women who received maternity care and gave birth in 2015–2018 in Bulgaria (n = 4951), Romania (n = 2018), and Moldova (n = 376) provided information on their experiences with the care received. We used regression analysis to identify factors associated with accessing maternity care across the three countries.ResultsResults show high rates of cesarean births (CB) and a low number of antenatal and postnatal care visits. Informal payments and use of personal connections are common practices. Formal and informal out‐of‐pocket payments create a financial burden for women with health complications. Women who had health complications, those who gave birth by cesarean, and women who gave birth in a public facility and had fewer antenatal check‐ups, were more likely to describe facing access‐related barriers.ConclusionsThis study identifies several barriers to high‐quality maternity care in Romania, Bulgaria and Moldova. More attention should be paid to the appropriateness of care provided to women with complicated pregnancies, to those who have CBs, to women who give birth in public facilities, and to those who receive fewer antenatal care visits.
Shifting risk‐stratified early prostate cancer detection to a primary healthcare setting
Authors: Hogenhout Renée , Osses Daniël F. , Alberts Arnout R. , Buizer‐Rijksen Hanne G. , Remmers Sebastiaan , Roobol Monique J. 
Published: November 2022

ObjectiveTo evaluate the feasibility of multivariable risk stratification for early prostate cancer (PCa) detection in a primary healthcare diagnostic facility with regard to its effects on the referral rate and subsequent PCa diagnoses compared to a PSA threshold of 3.0 ng/mL as the current referral indicator.Patients and MethodsIn 2014, the Erasmus MC Cancer Institute and the primary healthcare diagnostic facility STAR‐SHL (located in Rotterdam city centre) initiated this observational study, in which general practitioners (GPs) could refer men who wished to undergo PCa screening to STAR‐SHL for consultation by specially trained personnel. Referral recommendations to secondary healthcare were based on the outcome of application of the Rotterdam Prostate Cancer Risk Calculator (RPCRC) and were compared to the current Dutch GPs' PSA referral threshold of 3.0 ng/mL. For data collection on PCa diagnoses, the study cohort was linked to the Dutch nationwide pathology databank (PALGA).ResultsBetween January 2014 and February 2021, 507 men were referred for consultation and in 495 men prostate‐specific antigen (PSA) was tested. The median (interquartile range) follow‐up from consultation to PALGA linkage was 43 (25–65) months. In total, 279 men (56%) had a PSA level ≥3.0 ng/mL, of whom 68% (95% confidence interval [95% CI] 63–74) were considered at low risk according to the RPCRC. Within 1 year after consultation, one of these men (0.52%; 95% CI 0.092–2.9) was diagnosed with clinically significant (cs)PCa (i.e., International Society of Urological Pathology Grade Group ≥2). Thereafter, another four (2.1%; 95% CI 0.82–5.3) low‐risk men were diagnosed with csPCa. Of the high‐risk men who were biopsied within 1 year after consultation (n = 61), 77% (95% CI 65–86) were diagnosed with PCa and 49% (95% CI 37–61) with csPCa.ConclusionIn a primary healthcare diagnostic facility, the RPCRC could reduce up to 68% of referrals to secondary healthcare, as compared to a PSA referral threshold of 3.0 ng/mL. Deploying the RPCRC in this setting resulted in a high csPCa detection rate in those men biopsied. This strategy can be considered safe since the observational data showed low proportions of csPCa among men at low risk.
The fate of interneurons, GABAA receptor sub‐types and perineuronal nets in Alzheimer's disease
Authors: Ali Afia B. , Islam Anam , Constanti Andrew 
Published: November 2022

AbstractAlzheimer's disease (AD) is the most common neurological disease, which is associated with gradual memory loss and correlated with synaptic hyperactivity and abnormal oscillatory rhythmic brain activity that precedes phenotypic alterations and is partly responsible for the spread of the disease pathology. Synaptic hyperactivity is thought to be because of alteration in the homeostasis of phasic and tonic synaptic inhibition, which is orchestrated by the GABAA inhibitory system, encompassing subclasses of interneurons and GABAA receptors, which play a vital role in cognitive functions, including learning and memory. Furthermore, the extracellular matrix, the perineuronal nets (PNNs) which often go unnoticed in considerations of AD pathology, encapsulate the inhibitory cells and neurites in critical brain regions and have recently come under the light for their crucial role in synaptic stabilisation and excitatory‐inhibitory balance and when disrupted, serve as a potential trigger for AD‐associated synaptic imbalance. Therefore, in this review, we summarise the current understanding of the selective vulnerability of distinct interneuron subtypes, their synaptic and extrasynaptic GABAAR subtypes as well as the changes in PNNs in AD, detailing their contribution to the mechanisms of disease development. We aim to highlight how seemingly unique malfunction in each component of the interneuronal GABA inhibitory system can be tied together to result in critical circuit dysfunction, leading to the irreversible symptomatic damage observed in AD.
Cells with stem‐like properties are associated with the development of HPV18‐positive cervical cancer
Authors: Kusakabe Misako , Taguchi Ayumi , Tanikawa Michihiro , Wagatsuma Ryota , Yamazaki Miki , Tsuchimochi Saki , Toyohara Yusuke , Kawata Akira , Baba Satoshi , Ueno Toshihide , Sone Kenbun , Mori‐Uchino Mayuyo , Ikemura Masako , Matsunaga Hiroko , Nagamatsu Takeshi , Wada‐Hiraike Osamu , Kawazu Masahito , Ushiku Tetsuo , Takeyama Haruko , Oda Katsutoshi , Kawana Kei , Mano Hiroyuki , Osuga Yutaka 
Published: November 2022

AbstractThe cellular origins of cervical cancer and the histological differentiation of human papillomavirus (HPV)‐infected cells remain unexplained. To gain new insights into the carcinogenesis and histological differentiation of HPV‐associated cervical cancer, we focused on cervical cancer with mixed histological types. We conducted genomic and transcriptomic analyses of cervical cancers with mixed histological types. The commonality of the cellular origins of these cancers was inferred using phylogenetic analysis and by assessing the HPV integration sites. Carcinogenesis was estimated by analyzing human gene expression profiles in different histological types. Among 42 cervical cancers with known HPV types, mixed histological types were detected in four cases, and three of them were HPV18‐positive. Phylogenetic analysis of these three cases revealed that the different histological types had a common cell of origin. Moreover, the HPV‐derived transcriptome and HPV integration sites were common among different histological types, suggesting that HPV integration could occur before differentiation into each histological type. Human gene expression profiles indicated that HPV18‐positive cancer retained immunologically cold components with stem cell properties. Mixed cervical cancer has a common cellular origin among different histological types, and progenitor cells with stem‐like properties may be associated with the development of HPV18‐positive cervical cancer.
Enlightening through children—playing and thinking together in new ways
Authors: Saal Britta 
Published: November 2022

AbstractThe undertaking in this article is primarily to recall the basic meaning of enlightenment as a self‐active thinking movement. I show that enlightenment is not something to be taught, but rather the encouragement to think for yourself and, subsequently, to think anew; and this applies to the same degree to children and adults. I suggest a childist critique of enlightenment by addressing, first, enlightenment's overlooked adultist conception, giving, second, a brief practical insight into the children's polylogue, and, finally, re‐adjusting the understanding of enlightenment by referring to Hannah Arendt's considerations about world‐building and John Wall's thoughts about play.
Screening for obstructive sleep apnea before coronary angiography
Authors: Pei Guo , Ou Qiong , Chen Yongchi , Xu Yanxia , Tan Jiaoying 
Published: November 2022

AbstractIntroductionThe prevalence of obstructive sleep apnea (OSA) in patients with suspected coronary heart disease (CHD) is yet to be clarified. This study aimed to investigate the prevalence of OSA before coronary angiography (CAG).MethodsWe retrospectively evaluated patients with suspected CHD admitted to the Department of Geriatric Cardiology of our hospital between July 2019 and July 2021. OSA was screened using the level III home sleep apnea test before CAG. The prevalence of OSA was then compared between the CHD and non‐CHD groups. CHD severity was determined using the Gensini score of CAG results, and OSA severity was graded using the apnea‐hypopnea index (AHI).ResultsAmong the 327 patients, 211 had CHD. In total, 264 patients were diagnosed with OSA (80.7%) (184 patients, CHD group [87.2%]; 80 patients, non‐CHD group [69.0%]). The CHD group had a significantly higher prevalence of OSA (P < 0.01) and higher AHI (CHD group 18.76 ± 14.94, non‐CHD group 11.56 ± 10.67, P < 0.01). The Gensini score was positively correlated with OSA severity in patients with CHD, and AHI ≥ 20 was a risk factor for CHD (odds ratio: 1.961, 95% confidence interval: 1.065–3.608, P < 0.05).ConclusionOSA screening before CAG revealed a higher prevalence in CHD patients than in non‐CHD patients. The degree of coronary artery obstruction is positively correlated with AHI, and AHI ≥ 20 is a risk factor for CHD. Therefore, attention should be paid to OSA screening and management before CAG in patients with suspected CHD.
Plasma levels of E‐selectin are associated with retinopathy in sickle cell disease
Authors: Agouti Imane , Masson Elodie , Loundou Anderson , Jean Estelle , Arnaud Laurent , Abdili Evelyne , Berenger Patricia , Lavoipierre Virginie , Séguier Julie , Dignat‐George Françoise , Lacroix Romaric , Bernit Emmanuelle 
Published: November 2022

AbstractBackgroundThe vascular endothelium is markedly disrupted in sickle cell disease (SCD) and is the converging cascade of the complex pathophysiologic processes linked to sickle cell vasculopathy. Circulating endothelial activation and/or apoptotic markers may reflect this endothelial activation/damage that contributes to the pathophysiology of the SCD vascular complications.MethodsPlasmatic levels of circulating endothelial cells (CECs), E‐selectin, progenitor's endothelial cells (EPCs), and circulating extracellular vesicles (EVs) were evaluated in 50 SCD patients, 16 with vasculopathy. The association between these markers and the occurrence of disease‐related microvascular injuries of the eye (retinopathy), kidney (nephropathy), and skin (chronic active ulcers) was explored.ResultsAmong the endothelial activation markers studied, only higher plasma levels of E‐selectin were found in SCD patients with vasculopathy (p = .015). Increased E‐selectin levels were associated with retinopathy (p < .001) but not with nephropathy or leg ulcers. All patients, at steady state, with or without vasculopathy, did not display a high count of CEC and EPC, markers of endothelial injury and repair. We did not show any significant differences in EVs levels between vasculopathy and not vasculopathy SCD patients.ConclusionsFurther studies will be required to determine whether the E‐selectin could be used as an early biomarker of retinopathy sickle cell development.
Creep life estimation of a service‐exposed P91 cross‐weld joint based on a primary‐secondary creep deformation model
Authors: Lok Vanno , Le Thi Giang , Yoon Kee Bong 
Published: November 2022

AbstractCreep deformation and rupture behavior of welded joints from service‐exposed P91 steel were investigated by creep tests and microstructure examination. A series of miniature tensile creep tests were conducted at 569°C under applied stresses ranging from 182.5 to 286 MPa for the heat‐affected zone (HAZ), base metal, and weld metal. Creep deformation behavior was modeled using the experimentally measured creep strain data as the primary creep‐secondary creep (PC‐SC) model and fitting closely with the measured creep curves of each metal. Power‐law type creep equations were employed to describe the primary and secondary creep behavior. The creep rupture life of the cross‐weld specimen was predicted using the PC‐SC model with HAZ properties. An accurate prediction was possible if the stress in estimation was increased to 1.14 times of the actual applied stress. The creep test results with the cross‐weld specimens were used for verification. Applying the Monkman–Grant relationship and the creep damage tolerance factors to the cross‐weld joint life prediction are also discussed.
Parameter estimation in multiaxial fatigue short crack growth model using hierarchical Bayesian linear regression
Authors: He Gao Yuan , Zhao Yong Xiang , Yan Chu Liang 
Published: November 2022

AbstractMultiaxial fatigue failure is the most common problem in engineering structures. It is worth noting that short crack growth accounts for most of the fatigue life. Hence, it is necessary to study the short crack growth models for multiaxial fatigue life assessment. The primary focus of this study is to develop a hierarchical Bayesian linear regression method to estimate parameters in multiaxial fatigue crack growth model. The Bayesian method is used to estimate the intercept and slope of the regression equation for each loading path and ensemble test datasets. The method of this work was demonstrated on three multiaxial fatigue crack growth datasets. The main results obtained in this paper were that the parameters of the multiaxial fatigue crack growth model changed significantly with different loading paths, and the parameters of the model depended on the multiaxial loading path.
The potential bias of nitrogen deposition effects on primary productivity and biodiversity
Authors: Ke Yuguang , Yu Qiang , Wang Hongqiang , Zhao Yi , Jia Xiaotong , Yang Yadong , Zhang Yunlong , Zhou Wei , Wu Honghui , Xu Chong , Sun Tao , Gao Yingzhi , Jentsch Anke , He Nianpeng , Yu Guirui 
Published: November 2022

AbstractAtmospheric nitrogen (N) deposition is composed of both inorganic nitrogen (IN) and organic nitrogen (ON), and these sources of N may exhibit different impacts on ecosystems. However, our understanding of the impacts of N deposition is largely based on experimental gradients of INs or more rarely ONs. Thus, the effects of N deposition on ecosystem productivity and biodiversity may be biased. We explored the differential impacts of N addition with different IN:ON ratios (0:10, 3:7, 5:5, 7:3, and 10:0) on aboveground net primary productivity (ANPP) of plant community and plant diversity in a typical temperate grassland with a long‐term N addition experiment. Soil pH, litter biomass, soil IN concentration, and light penetration were measured to examine the potential mechanisms underlying species loss with N addition. Our results showed that N addition significantly increased plant community ANPP by 68.33%–105.50% and reduced species richness by 16.20%–37.99%. The IN:ON ratios showed no significant effects on plant community ANPP. However, IN‐induced species richness loss was about 2.34 times of ON‐induced richness loss. Soil pH was positively related to species richness, and they exhibited very similar response patterns to IN:ON ratios. It implies that soil acidification accounts for the different magnitudes of species loss with IN and ON additions. Overall, our study suggests that it might be reasonable to evaluate the effects of N deposition on plant community ANPP with either IN or ON addition. However, the evaluation of N deposition on biodiversity might be overestimated if only IN is added or underestimated if only ON is added.
Environmental DNA reveals a mismatch between diversity facets of Amazonian fishes in response to contrasting geographical, environmental and anthropogenic effects
Authors: Coutant Opale , Jézéquel Céline , Mokany Karel , Cantera Isabel , Covain Raphaël , Valentini Alice , Dejean Tony , Brosse Sébastien , Murienne Jérôme 
Published: November 2022

AbstractFreshwater ecosystems are among the most endangered ecosystem in the world. Understanding how human activities affect these ecosystems requires disentangling and quantifying the contribution of the factors driving community assembly. While it has been largely studied in temperate freshwaters, tropical ecosystems remain challenging to study due to the high species richness and the lack of knowledge on species distribution. Here, the use of eDNA‐based fish inventories combined to a community‐level modelling approach allowed depicting of assembly rules and quantifying the relative contribution of geographic, environmental and anthropic factors to fish assembly. We then used the model predictions to map spatial biodiversity and assess the representativity of sites surveyed in French Guiana within the EU Water Framework Directive (WFD) and highlighted areas that should host unique freshwater fish assemblages. We demonstrated a mismatch between the taxonomic and functional diversity. Taxonomic assemblages between but also within basins were mainly the results of dispersal limitation resulting from basin isolation and natural river barriers. Contrastingly, functional assemblages were ruled by environmental and anthropic factors. The regional mapping of fish diversity indicated that the sites surveyed within the EU WFD had a better representativity of the regional functional diversity than taxonomic diversity. Importantly, we also showed that the assemblages expected to be the most altered by anthropic factors were the most poorly represented in terms of functional diversity in the surveyed sites. The predictions of unique functional and taxonomic assemblages could, therefore, guide the establishment of new survey sites to increase fish diversity representativity and improve this monitoring program.