bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lamin B1 curtails early human papillomavirus infection by safeguarding nuclear compartmentalization and autophagic capacity
Authors: Molenberghs Freya , Verschuuren Marlies , Vandeweyer Lauran , Peeters Sarah , Bogers Johannes , Novo Claudina Perez , Berghe Wim Vanden , De Reu Hans , Cools Nathalie , Schelhaas Mario , De Vos Winnok 
Published: August 2022

Abstract
Human papillomavirus (HPV) infection is the prime elicitor of cervical and head-and-neck cancers. The HPV genome enters the nucleus during mitosis when the nuclear envelope dismantles. Since lamins safeguard nuclear integrity during interphase, we asked to what extent their loss would affect early HPV infection. We challenged human cervical cancer cells knocked out for the major lamin genes with a HPV16 pseudovirus (PsV) encoding an EGFP reporter and found that loss of lamin B1 amplified infection rate. A prolonged mitotic window and extensive nuclear rupture propensity during interphase led to a higher nuclear PsV load in LMNB1 knockout cells, but unchanged EGFP transcript levels pointed to an additional defect in protein turnover. We found a strong decrease in autophagic capacity in LMNB1 knockout cells, which we connect to the persistent activation of cGAS. Thus, loss of lamin B1 increases nuclear perviousness and blunts the autophagic capacity, which primes cells for unrestrained buildup of HPV capsids.
Investigating doctors' involvement to ACC/AHA 2018 blood cholesterol treatment recommendations at Al-Qurryyat General Hospital, in Northern Saudi Arabia
Authors: AlHindi yosra , Alanazi Radhi Mufadhi 
Published: August 2022

Abstract
Background: The updated 2018 American College of Cardiology (ACC) and American Heart Association (AHA) blood cholesterol treatment guideline showed a major shift in treating atherosclerotic cardiovascular disease (ASCVD). In 2018 they recommendations were adopted to increase the number of persons using statin therapy, but the real-world observations especially in Saudi Arabia of how treatment use has changed has been limited in the literature. Doctors' involvement to ACC/AHA 2018 recommendations regarding prescribing medications have not been studied in Saudi Arabia.· Aim: To examine doctors' involvement to ACC/AHA 2018 cholesterol recommendations in managing patients with any acute atherosclerotic cardiovascular disease. How they follow the role of prescribing statin medications and the proper doses for statin or other anti-cholesterol medications.· Results: Around 47% of the patients were managed according to the 2018 ACC/AHA recommendations. On the other hand, 53% of patients did not take statin. Doctors found to be adhering to the recommendations when managing patients with myocardial infarction and unstable angina in contrast to other astherovascular events. For the non-adherent patients 40% of them refused because of their own believes or the fear of the side effects.· Conclusion: our primary analysis concluded that around more than half of the patients with acute atherosclerotic diseases were not given the recommended statin by the 2018 ACC/AHA blood cholesterol recommendations, moreover some of them did not receiving any statin therapy.
compartment syndrome of the hand: a rare localization with a poor prognosis: two case reports and a literature review
Authors: bouziane walid , karbal jamal , agoumi omar , daoudi abdelkrim 
Published: August 2022

Abstract
Introduction:
Compartment syndrome is a medical-surgical emergency. In fact, it is a "race against the clock" to reduce, as quickly as possible, the pressure inside a well-defined muscular compartment. Its development at the hand level as part of a crushing trauma is a rare localization and mainly affects the radial interosseous muscular compartment. The diagnosis of hand compartment syndrome is primarily clinical. Its classical clinical triad includes spontaneous pain, paralysis, and pain increase during passive extension. Any additional examination must not, in any sense, delay the patient's management. Ignoring this surgical emergency can cause permanent damage to the hand.
Case report:
In this article, we will discuss our experience with two cases who presented with hand compartment syndrome after suffering numerous metacarpal fractures as a result of trauma. The treatment included a discharge dermofasciotomy, metacarpal pinning, and close hand surveillance. Excellent outcomes were seen after three months, with complete functional recovery of hand mobility.
Conclusion
The compartment syndrome in the hand is a rare localization that affects the hand's functional prognosis. Its treatment consists of two key components: discharge aponeurotomy and bone stabilization. There should be no delay in surgical treatment for this syndrome.
Minimizing JIT scheduling of unrelated parallel machine with family setups and soft time window constraints
Authors: Shafipour Milad , Rezaeian Javad , Foroutan Reza Alizadeh 
Published: August 2022

Abstract

Multi-machine scheduling has been one of the best-known and practical problems in the last decade and its applications are constantly increasing. This study addresses an unrelated parallel machine scheduling problem with family setups and soft time windows, in which machine eligibility and precedence constraints are considered. The objective is to minimize the total of weighted early and tardy costs. The problem is investigated for different sizes of jobs, families and machines. Two different metaheuristic algorithms, a simulated annealing (SA) and an artificial immune system (AIS) are presented. Two strategies called repair and penalty are proposed to implement predecessor constraints. Some computational experiments are performed and one-way analysis of variance (ANOVA) is conducted to compare the performance of the proposed metaheuristics and evaluate the designed combinations in terms of objective values and computational (CPU) times. Results demonstrate that SA with repair strategy generally outperforms other proposed methods.
Microbial responses to soil cooling might explain increases in microbial biomass in winter
Authors: Schnecker Jörg , Spiegel Felix , Li Yue , Richter Andreas , Sandén Taru , Spiegel Heide , Zechmeister-Boltenstern Sophie , Fuchslueger Lucia 
Published: August 2022

Abstract

In temperate soil systems, microbial biomass often increases during winter and decreases again in spring. This build-up and release of microbial carbon could potentially lead to a stabilization of soil carbon during winter times. Whether this increase is caused by changes in microbial physiology, in community composition or by changed substrate allocation within microbes or communities is unclear. In a laboratory incubation study, we looked into microbial respiration and growth, as well as microbial glucose uptake and carbon resource partitioning in response to cooling. Soils taken from a temperate forest and an agricultural system in October 2020, were cooled down from field temperature of 11 °C to 1 °C. We determined microbial growth using 18O-incorporation into DNA immediately after cooling and after an acclimation phase of 7 days; in addition, we traced 13C-labelled glucose into microbial biomass, CO2 respired from the soil, and into microbial phospholipid fatty acids (PLFAs). Our results show that the studied soil microbial communities responded immediately to soil cooling. Independent of soil type and acclimation period, total respiration, as well as 18O based growth, and thus cell division were strongly reduced when soils were cooled from 11 °C to 1 °C, while glucose uptake and glucose-derived respiration were unchanged. We found that microbes increased the investment of glucose-derived carbon in unsaturated phospholipid fatty acids at colder temperatures. Since unsaturated fatty acids retain fluidity at lower temperatures compared to saturated fatty acids, this could be interpreted as a precaution to reduced temperatures. Together with the maintained glucose uptake and reduced cell division, our findings show an immediate response of soil microorganisms to soil cooling, potentially to prepare for freeze-thaw events. The discrepancy between C uptake and cell division, further hints at a mechanism that could explain previously observed high microbial biomass carbon in temperate soils in winter.
Postindustrial landscapes are neglected localities which may play an important role in the urban ecology of ticks and tick-borne diseases
Authors: Dvořáková Anna , Klímová Anita , Hrnková Johana , Černý Jiří 
Published: August 2022

Abstract
Background
Numerous recent studies have shown that ticks and tick-borne pathogens pose a considerable threat in urban areas, such as parks, playgrounds, zoos, cemeteries etc. Abandoned postindustrial localities, and other types of vague terrain, are another example of urban wilderness areas which have been absolutely neglected in respect to ticks and tick-borne pathogens thus far, even though they provide ideal biotopes for ticks.
Methods
The abundance of ticks and prevalence of Borrelia burgdorferi sensu lato spirochetes were compared between a city park and an adjacent abandoned construction waste disposal site in Prague, Czechia during the 2021 tick season.
Results
The results showed that ticks and borrelia spirochetes are present at the city park as well as at the abandoned construction waste disposal site, although, in lower numbers.
Discussion
According to the best of our knowledge, this is the first report describing the presence of ticks and tick-borne pathogens in an urban postindustrial landscape. More detailed studies are needed to uncover the role of these localities in the ecology of ticks and ecoepidemiology of tick-borne diseases in urban areas.
High-accuracy and high-speed calculation of second-order ODE by the interpolation finite difference method
Authors: Fukuchi Tsugio 
Published: August 2022

Abstract

Numerical calculation of differential equations using the finite difference method (FDM) is entering a new phase. The high-accuracy calculation system of the interpolation FDM (IFDM) enables extremely high-accuracy numerical calculation. One of the higher-order difference schemes of IFDM is defined as the compact interpolation finite difference (CIFD) scheme. According to this finite difference (FD) scheme, it becomes clear that it can be calculated with 15 significant figures in the numerical analysis of the 1D Poisson equation under double precision calculation. Thus, it is possible to perform an apparent error-free calculation. The 1D Poisson equation is a special form of the second-order ordinary differential equation (ODE), but this paper shows that the methods reported thus far are generalized to the general second-order ODE.
Gaps in research and capacity development for malaria surveillance and response in the Asia- Pacific: meeting report
Authors: Sirimatayanant Massaya , Hein Phone Si , Anderson Laura Fay , Montoya Lucía Fernández , Potter Rebecca , Nghipumbwa Mwalenga , Ranaweera Prasad , Ngor Pengby , Phetsouvanh Rattanaxay , Maude Richard James 
Published: August 2022

Abstract
Background
In light of the 2030 regional malaria elimination goal, there is an urgency for Asia-Pacific countries to accelerate progress towards national elimination and prevent re-establishment. The Asia Pacific Malaria Elimination Network (APMEN) Surveillance Response Working Group (SRWG) supports elimination goals of national malaria control programmes (NMCPs) by expanding the knowledge base, guiding the region-specific operational research agenda and addressing evidence gaps to improve surveillance and response activities.
Method
An online annual meeting was hosted from 1–3 November 2021, to reflect on research needed to support malaria elimination in the region, challenges with malaria data quality and integration, current surveillance-related technical tools, and training needs of NMCPs to support surveillance and response activities. Facilitator-led breakout groups were held during meeting sessions to encourage discussion and share experience. A list of identified research priorities was voted on by attendees and non-attending NMCP APMEN contacts.
Findings
127 participants from 13 country partners and 44 partner institutions attended the meeting, identifying strategies to address malaria transmission amongst mobile and migrant populations as the top research priority, followed by cost effective surveillance strategies in low resource settings, and integration of malaria surveillance into broader health systems. Key challenges, solutions and best practices for improving data quality and integrating epidemiology and entomology data were identified, including technical solutions to improve surveillance activities, guiding priority themes for hosting informative webinars, training workshops and technical support initiatives. Inter-regional partnerships and SRWG-led training plans were developed in consultation with members to be launched from 2022 onwards.
Conclusion
The 2021 SRWG annual meeting provided an opportunity for regional stakeholders, both NMCPs and APMEN partner institutions, to highlight remaining challenges and barriers and identify research priorities pertaining to surveillance and response in the region, and advocate for strengthening capacity through training and supportive partnerships.
Flood vulnerability assessment at the local scale using remote sensing and GIS techniques: a case study in Da Nang City, Vietnam
Authors: Thi An Tran , Izuru Saizen , Narumasa Tsutsumida , Raghavan Venkatesh , Hanh Le Ngoc , Van An Nguyen , Long Nguyen Vinh , Thi Thuy Ngo , Minh Truong Phuoc 
Published: August 2022

Abstract
This paper has developed a cost-efficient framework for flood vulnerability assessment at a local scale using a multi-parametric approach integrated with the Open Source Geographical Information System (GIS) and Open Remote Sensing data. The study focuses on generating a set of criteria considering three dimensions of flood vulnerability: exposure, sensitivity, and adaptive capacity (AC) on an index-based approach. These indicators were decided based on a robust analysis considering the physical and socio-economic conditions of the study area. The flood exposure was generated from the geomorphological and hydrological parameters integrated with the flood water depth, the distance to river channels, and the Modified Normalized Difference Water Index. The flood sensitivity was determined by the aggregation of local income, land use, poverty index, population density, and other parameters reflecting the socio-economic condition. The AC has been evaluated based on the Normalized Difference Vegetation Index, the density of the community service facilities, and other factors related to the coping capacity to flood. Finally, the flood vulnerability at the local scale was determined based on the integration of its contributing factors using the Analytical Hierarchical Process-based aggregated model. Results indicated that a total of 20 parameters impacted the flood vulnerability of the research area. The findings also confirmed that among the indicators of flood vulnerability of Da Nang City, the flood depth, land-use condition, and drainage system are the key factors affecting the vulnerability level. The empirical assessment showed that the study area is significantly affected by flood vulnerability with more than 60% of the area having the vulnerability level from moderate to very high. In addition, this paper points out that the vulnerability research should be localized and is not always based on the administrative units. This practice can make the decision-making process and adaptation plan more appropriate locally. Especially, this study attempted to evaluate the accuracy of the flood vulnerability map for the first time by using field survey data and the statistical report on flood damage that most of the previous studies have not conducted yet. This framework provides a valuable toolkit for flood management in data-scarce regions all over the world.
GIS-based spatio-temporal assessment of forest cover change and carbon sequestrations of District Abbottabad, Pakistan
Authors: Goheer Muhammad Arif , Hassan Sher Shah , Gul Kaenat , Waqar Mahnoor , Parveen Nazia 
Published: August 2022

Abstract
Forest plays an important role in the climate of a country and ecosystem's balance. During the last decade, District Abbottabad of Pakistan has gone through extensive land-use changes due to accelerated development, urbanization, and agriculture. This study examines the change in forest cover due to environmental factors with the help of satellite images (Landsat, Sentinel) and their classification through supervised classification, as well as the application of change detection technique after classification using geographical information system (GIS) over the last three decades (1986–2019). The land-use transition matrix was calculated from the year 1986 to 2019. The result shows an overall significant increase of 3.17, 17.24, and 7.24% in the forest, vegetation, and built-up areas, respectively; whereas, water bodies and others (barren land) have decreased significantly by 0.69 and 26.96%, respectively. This study also provides an assessment of the incline of carbon stock from the forest area over the period of the study by 17.19%.
The Effect of Whole Blood and Bone Marrow with the Addition of
Pyrimidine-2,4(1h,3h)-dione Thietanyl Derivatives on Free Radical
Oxidation
Authors: Meshcheryakova Svetlana , Kayumova Aliya , Kang Yang , Shumadalova Alina , Vinogradova Yulia , Khuzin Dinislam , Ziyakaeva Klara , Kiseleva Olga , Gabdulkhakova Irina , Beylerli Ozal , Gareev Ilgiz , Sufianov Albert , Sufianova Galina , Ahmad Aamir , Yang Guang , Guo Zidan 
Published: August 2022

Background:
It is relevant to study the general patterns and identify non-specific mechanisms
of body protective and adaptive reactions violation, which can lead to the various pathological processes
and develop principles for the correction of these disorders. One of the therapy and prevention directions
is the search for new medicines. In recent years, new derivatives of pyrimidine bases have been synthesized
and studied. Pyrimidine-based medicines have a membrane-stabilizing and immunomodulatory effect
and can normalize metabolic disorders and increase the oxidative activity of leukocytes. Disruption
of the free radical oxidation processes, the generation of reactive oxygen species and lipid peroxidation,
including in whole blood and bone marrow, has gained importance in recent years.

Methods:
Each reaction was monitored by thin layer chromatography. 1H, 13C, and 15N NMR spectra
were recorded (chemical shifts were expressed as δ-values). We studied the effect of 6-methyl-3-(thietan-
3-yl)pyrimidine-2,4(1H,3H)-dione on the generation of reactive oxygen species (ROS) in the whole
blood and bone marrow using the study of whole blood spontaneous and stimulated chemiluminescence
(CL). CL methods make it possible to quickly and easily assess the studied material (whole blood, bone
marrow) effect on free radical oxidation. Using CL methods, it is possible to reveal the presence of
medicines' pro- or antioxidant properties, opening up new possibilities in the search for substances with
antioxidant properties and comparing their activity.

Results:
Alkylation of 6-methylpyrimidine-2,4(1H,3H)-dione by 2-chloromethylthiirane in protic solvents
in the presence of alkali leads to the formation of an N-thietane derivative. NMR spectroscopy
showed that 6-methylpyrimidine-2,4(1H,3H)-dione was alkylated at position 3. The oxidation reactions
of N-(thietan-3-yl)pyrimidine-2,4(1H,3H)-dione were studied, and it was determined that, depending on
the excess of the oxidizing agent and the duration of the process, N-(1-oxothietan-3-yl)- or N-(1,1--
dioxothietan-3-yl)pyrimidine-2,4(1H,3H)-diones were formed. The effects of free radical oxidation processes
of new biologically active pyrimidine-2,4(1H,3H)-diones were studied.

Conclusion:
New pyrimidine-2,4(1H,3H)-diones increase the general adaptive capabilities of the body
and have protective effects in extreme conditions.
Assessing the Influence of Salvia triloba on Memory Deficit Caused by
Sleep Deprivation in the Context of Oxidative Stress
Authors: Massadeh Adnan M. , Alzoubi Karem H. , Melhim Amal M. , Rababa’h Abeer M. 
Published: August 2022

Background:
Learning and memory deficit has been reported to be correlated to oxidative
mutilation in the hippocampus. Moreover, sleep deprivation (SD) mitigates memory via distressing
oxidative stress balance. In the current report, the prospective neuroprotective role of oral sage (Salvia
triloba) extract on cognitive impairment induced by chronic SD was investigated.

Methods:
The SD was induced in adult male Wistar rats employing a modified multiple platform (8
h/day; for six weeks). Simultaneously, S. triloba extract (375 mg/kg, orally) was administered for six
weeks. Thereafter, the Radial Arm Water Maze test was utilized to evaluate spatial learning and
memory. Moreover, activities of different hippocampal antioxidant parameters: glutathione peroxidase
(GPx), oxidized glutathione (GSSG), reduced glutathione (GSH), catalase, superoxide dismutase
(SOD), and the thiobarbituric acid reactive substance (TBARS) were measured in rats’ hippocampus.
Moreover, the level of brain derived neurotrophic factor (BDNF) was assessed.

Results:
Current results illustrate that chronic SD significantly compromised both memories, shortand
long-term, while sage extract inhibited these consequences. Moreover, sage extract remarkably
stabilized the antioxidant enzyme levels, which were decreased by SD, such as: SOD, catalase, and
GPx (P <0.05), and remarkably augmented the GSH/GSSG ratio in SD rats (P < 0.05). However, no
substantial alterations of GSH, TBARS or BDNF levels (P > 0.05) were seen with sage extract administration.

Conclusion:
Chronic treatment with sage extract (S. Triloba) precluded SD-induced memory impairment
by regularizing antioxidant parameters levels in rats’ hippocampus.
Outcomes of Revision Arthroplasty for Hip Joint Infection in Matched
Groups of HIV-Positive and HIV-Negative Patients
Authors: Triapichnikov Alexander S. , Ermakov Artem M. , Malkova Tatiana A. 
Published: August 2022

Background:
Several studies reported on periprosthetic infection after primary THA in
HIV-positive patients, but very few showed the results of its revision.

Objective:
The aim was to compare primary and secondary clinical outcomes after revision arthroplasty
for hip joint infection in matched groups of HIV-infected and HIV-negative patients.

Methods:
Using the hospital database, thirteen HIV-positive patients (13 infected hips) and thirteen
HIV-negative patients of the matched control group (13 infected hips) were identified and their records
were studied retrospectively. They underwent revision surgery aimed at infection arrest and
total hip replacement due to infection developed after primary THA or infected spacers. Harris Hip
Score, reinfection rate, limb shortening, and definite outcomes were evaluated with Wilcoxon,
Mann-Whitney, and Chi-squared tests.

Results and Discussion:
Spacers with antibiotics were implanted in patients of both groups at the
first step of revision, except for one HIV-infected patient who had resection arthroplasty. The mean
follow-up was 29.4 ± 2.7 and 33. ± 2.9 months for the HIV-group and control group, respectively.
Three patients of the HIV-group completed two-stage revision arthroplasty versus ten patients from
the control group. Re-infection rate was higher in the HIV-group. At final follow-ups, the mean
HHS was significantly different (53 ± 3.2 points in the HIV-group versus 79.14 ± 3.1 points in the
control group) along with limb length discrepancy (3.71 ± 0.43 versus 1.4 ± 0.32).

Conclusion:
Both primary and secondary clinical outcomes of revision arthroplasty for hip joint infection
in HIV-positive patients were significantly worse than in the matched group of HIVnegative
patients. Revisions in HIV-positive group resulted in a higher reinfection rate, a small
number of definite two-stage revisions, and lower functional scores.