bluuebunny/crossref_metadata_2025_split huggingface dataset:
Da vinci robotic-assisted treatment of pediatric choledochal cyst
Authors: Chen Shan , Lin Yang , Xu Di , Lin Jianli , Zeng Yunlong , Li Lizhi 
Published: November 2022

ObjectiveTo evaluate the advantages and disadvantages of da Vinci robot and laparoscopy in treating pediatric choledochal cysts.MethodsWe retrospectively analyzed clinical data from forty-two children diagnosed with choledochal cysts in our hospital from January 2018 to January 2021. Twenty children underwent da Vinci robotic surgery, and the others underwent traditional laparoscopy. We compared differences in general information and preoperative, intraoperative, and postoperative differences between the two surgical groups.ResultsThere was no statistically significant difference in age, gender, weight, type, maximum cyst diameter, preoperative C-reactive protein (CRP) value, postoperative complication rate, and postoperative pain score between the two surgical groups (P &gt; 0.05). The average age of the robot-assisted group was 3.62 ± 0.71 years old (range = 1–12 years). There were nineteen cases of Todani type I, one patients of other types, and the maximum cyst diameter was 35.45 ± 9.32 mm (range = 12–56 mm). In the laparoscopic group, the average age was 3.08 ± 0.82 years old (range = 3–10 years). Twenty-one patients had Todani type I, and one had other types. The maximum cyst diameter was 31.94 ± 8.64 mm (range = 10–82 mm). The robot-assisted group had better abdominal drainage, postoperative CRP value, fasting time, and discharge time than the laparoscopic group (P &lt; 0.05).ConclusionCompared with laparoscopy, the da Vinci system has the advantages of less tissue damage, faster recovery, and better healing in the treatment of children with congenital choledochal cysts. With technological advancements and an increased number of experienced surgeons, robotic surgery may become a new trend in surgery.
Zuogui Wan ameliorates high glucose-induced podocyte apoptosis and improves diabetic nephropathy in db/db mice
Authors: Zhu Bingbing , Fang Ji , Ju Zhengcai , Chen Ying , Wang Li , Wang Hao , Xing Lina , Cao Aili 
Published: November 2022

Zuogui Wan (ZGW), a well-known traditional Chinese medicine (TCM), has been used to nourish “Kidney-Yin” for a long time in China, implying a protective effect on the kidney. The aim of the present study is to investigate the effect of ZGW on high glucose-induced podocyte apoptosis and diabetic nephropathy (DN) in db/db mice. ZGW (1 g/kg−1/day−1) was administered intragastrically to db/db mice for 8 weeks. HPLC was used for identifying the components of ZGW, biochemical and histopathological approaches were used for evaluating its therapeutic effects, and cultured mouse podocytes were used for further exploring its underlying mechanism in vitro. ZGW improved renal function and podocyte loss and also normalized kidney reactive oxygen species production in db/db mice. The cytotoxicity of ZGW on mouse podocytes was assessed by the LDH assay. The effect of ZGW on podocyte viability and apoptosis was determined with CCK-8 and Annexin-V/PI staining by treatment with high glucose. ZGW attenuated podocyte apoptosis, and oxidative stress was detected by the peroxide-sensitive fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) staining in a dose-dependent manner. Furthermore, ZGW decreased the expression of caspase-3 and phospho-p38 in both the kidney cortex and high glucose-treated podocytes. Thus, our data from in vivo and in vitro studies demonstrated that ZGW improved renal injury in diabetes by inhibiting oxidative stress and podocyte apoptosis.
Zebrafish cornea formation and homeostasis reveal a slow maturation process, similarly to terrestrial vertebrates’ corneas
Authors: Ikkala Kaisa , Raatikainen Sini , Koivula Henri , Michon Frederic 
Published: November 2022

Corneal blindness is the fourth leading cause of blindness worldwide. The superficial position of cornea on the eye makes this tissue prone to environmental aggressions, which can have a strong impact on sight. While most corneal pathology studies utilize terrestrial models, the knowledge on zebrafish cornea is too scarce to comprehend its strategy for the maintenance of a clear sight in aquatic environment. In this study, we deciphered the cellular and molecular events during corneal formation and maturation in zebrafish. After describing the morphological changes taking place from 3 days post fertilization (dpf) to adulthood, we analyzed cell proliferation. We showed that label retaining cells appear around 14 to 21dpf. Our cell proliferation study, combined to the study of Pax6a and krtt1c19e expression, demonstrate a long maturation process, ending after 45dpf. This maturation ends with a solid patterning of corneal innervation. Finally, we demonstrated that corneal wounding leads to an intense dedifferentiation, leading to the recapitulation of corneal formation and maturation, via a plasticity period. Altogether, our study deciphers the maturation steps of an aquatic cornea. These findings demonstrate the conservation of corneal formation, maturation and wound healing process in aquatic and terrestrial organisms, and they will enhance the use of zebrafish as model for corneal physiology studies.
Mental health problems of preschool children during the COVID-19 home quarantine: A cross-sectional study in Shanghai, China
Authors: Ma Chen-huan , Jiang Lian , Chu Li-ting , Zhang Chun-cao , Tian Yuan , Chen Jin-jin , Wang Yu 
Published: November 2022

ObjectiveAs the coronavirus disease 2019 (COVID-19) pandemic spread across Shanghai, China, in late February 2022 and protective measures to mitigate its impact were enacted, this study aimed to estimate how home quarantine affected the mental health of preschool children in Shanghai, China and explore the association between lifestyle factors and mental health during this special period.MethodsA cross-sectional online survey of 2,110 preschool students from Shanghai, China, was conducted during May 20–25,2022. Preschooler’ mental health (Strengths and Difficulties Questionnaire, SDQ) and daily activities were reported by parents.ResultsThe sample involved 2,110 children with a mean age of 4.65 years [standard deviation (SD): 0.91, range: 3–6 years]. Boys and children whose mother’s education level were college and high school had higher rate of mental health problems. Boys had significantly higher rates of peer problems and prosocial behaviors than girls. The 3-year-old group had significantly higher rates of prosocial behaviors than other groups. As compared to the Shanghai norm and the SDQ results of preschool children in Shanghai in 2019 (SH2019), there were a significant decrease in emotional symptoms score, as well as a significant increase in conduct problems score. Additionally, peer problems score significantly increased compared to SH2019. Decreased time spent on daily sleep was associated with the increased risk for preschoolers’ mental health problems.ConclusionThere was an increase in the frequency of emotional and behavioral problems, especially regarding conduct problems and peer problems, in preschool children during the COVID-19 home quarantine in Shanghai, China. Boys, younger preschool children and children whose mother’s education level were college and high school may be especially vulnerable to emotional and behavioral problems. It was also found that decreased time spent on sleep may aggravate preschool children’s mental health problems. It may be beneficial to differentiate and focus on conducting psychoeducation and implementing psycho-behavioral interventions to solve these issues.
Network connectivity between benevolent childhood experiences and uncertainty stress among Chinese university students
Authors: Pei Yifei , Wang Jingjing , Tang Jie , Yan Na , Luo Yunjiao , Xie Yaofei , Zhou Qin , Zhang Caiyi , Wang Wei 
Published: November 2022

BackgroundThe purpose of this study was to explore the association between benevolent childhood experiences (BCEs) and uncertainty stress among Chinese university students by network analysis.MethodsA total of 1,830 university students from three Chinese cities were recruited. Respondents' BCEs and uncertainty stress were self-reported using online questionnaire. The structure of the BCEs-uncertainty stress and related centrality indicators were examined for this sample.ResultsThe overall network model showed that “no ways to suit the important changes in life” was the most influential, followed by “all things are not going well,” “feel that there is nothing to do,” and “worry about the future.” And in this network, the most influential bridge symptom was “having a positive self-concept.”ConclusionThe central symptoms of the BCEs-uncertainty stress network should be prioritized as targets in interventions and prevention efforts to reduce uncertainty stress among Chinese university students. Improving university students' positive self-concept is important to alleviate the level of uncertainty stress among Chinese university students.
Distinct glycosylation and functional profile of typhoid vaccine-induced antibodies in a UK challenge study and Nepalese children
Authors: Stockdale L. K. , de Haan N. , Hill J. , Johnson M. , Tomic A. , Wuhrer M. , Jones E. , Jin C. , Nouta J. , Koeleman C. A. M. , Verheul M. , Basnyat B. , Shakya M. , Pant D. , Provstgaard-Morys S. , Pollard A. J. 
Published: November 2022

Vaccines against typhoid fever have been shown to be safe and effective in field trials. The mechanism through which the vaccines protect remains elusive. Recent data have implicated antibody glycosylation, and specifically afucosylated antibodies, as an important factor in vaccine-induced effector function for a range of viral infections, however this has not been evaluated for vaccines against bacterial infections such as Salmonella typhi. Here, we studied antibody glycosylation after either Vi-conjugate or Vi-polysaccharide vaccine in a UK cohort who were then challenged with virulent S. typhi, and compared findings to antibody glycosylation after Vi-conjugate vaccine in Nepalese children living in a typhoid endemic region. We compared vaccine-induced responses and correlated these measures with antibody-dependent function. Robust antigen-specific antibody galactosylation and sialylation modifications were induced by both vaccines in UK adults, with Vi-conjugate vaccine inducing Vi-specific glycan changes of higher magnitude than Vi-polysaccharide. Among those individuals diagnosed with typhoid fever after challenge, a distinct glycan profile was correlated with disease severity. Elevated galactosylation and sialylation was correlated with increased antibody-dependent phagocytosis by macrophages and neutrophils among UK adults. While bulk IgG glycosylation differed between Nepalese children and UK adults, vaccination with the Vi-conjugate vaccine overcame these differences to result in similar Vi-specific antibody glycosylation profiles 28 days after vaccination in both cohorts.
Evaluating clinical utility of subgingival and salivary endotoxin activity levels as periodontal biomarkers
Authors: Zaric Svetislav , Strachan Alexander , Kurushima Yuko , Dong Anbo , McIlwaine Clare , Harrington Zoe , Nibali Luigi , Foey Andrew , Ide Mark 
Published: November 2022

ObjectivesThe use of periodontal biomarkers for identification and monitoring of unique patient populations could foster better stratification of at-risk groups, increase access to treatment for those most in need, facilitate preventive measures and improve personalised care plans. The aim of this study was to examine the diagnostic and prognostic utility of oral lipopolysaccharides as bacterially-derived periodontal biomarkers.MethodsPeriodontal parameters were recorded, and saliva and subgingival plaque samples were collected at the beginning of the study from periodontally healthy volunteers and periodontitis patients, and three months after completion of conventional periodontal treatment in the periodontitis group. Endotoxin activity in the samples was measured using the recombinant factor C assay. Associations between clinical periodontal parameters and subgingival and salivary endotoxin activities were analysed using a multivariate regression model, while the ROC curve was applied to estimate the sensitivity, specificity and c-statistics for salivary and subgingival endotoxin activities as diagnostic biomarkers for periodontitis.ResultsSignificant correlations were found between subgingival endotoxin activities, probing pocket depth and periodontal diagnosis, which were independent from patients' age, gender and smoking status. In addition, subgingival endotoxin levels had high specificity and sensitivity in detecting periodontal health and disease (0.91 and 0.85 respectively). Salivary endotoxin activity was positively associated with periodontal diagnosis, mean probing pocket depth, percentages of sites over 4 mm and full mouth bleeding score. However, it was inferior in discriminating patients with stable periodontium from those with periodontitis (sensitivity = 0.69, specificity = 0.61) compared to subgingival endotoxin activity.ConclusionsSubgingival endotoxin activity has good diagnostic and prognostic values as a site-specific periodontal biomarker and is not influenced by the patient's age, gender or smoking status. In contrast, salivary endotoxin activity, as a patient-level biomarker, is dependent on patient's age, has poorer diagnostic and prognostic capability, but shows good correlations with disease susceptibility and both its extent and severity.
From physics of polar aurora to changes of the fundamental approaches to the physics of the magnetospheric processes
Authors: Antonova Elizaveta E. 
Published: November 2022

One of the main problems of modern magnetospheric physics is the lack of a self-consistent explanation of the main physical processes based on the laws of plasma physics. Among all the traditionally studied phenomena, the polar aurora stands out for being key to our understanding of several magnetospheric processes. In this manuscript, I would like to share with the younger generation my view about main auroral processes which I have developed during my career over the past 50 years.
Active exercise therapy improves the recovery of knee joint function and reduction of muscle atrophy after medial patellofemoral ligament reconstruction for recurrent patellar dislocation
Authors: Xing Dong , Li Wenyi , Yang Zhaoxu , Dong Zhijie , Kang Huijun , Wang Fei 
Published: November 2022

ObjectivesMedial patellofemoral ligament (MPFL) reconstruction is an important surgical therapy for recurrent patellar dislocation. However, few studies have focused on exercise therapy after MPFL reconstruction. Therefore, the first purpose was to compare the active and traditional postoperative exercise therapies on the recovery of knee joint function and reduction of muscle atrophy after MPFL reconstruction, and the second purpose was to compare the active and traditional postoperative exercise therapies on the patellar stability after MPFL reconstruction.MethodsThe cases of 31 patients with recurrent patellar dislocation treated with patella double semi-tunnel anatomical MPFL reconstruction from February 2016 and February 2019 were retrospectively reviewed. The clinical outcomes, including the patellar tilt angle (PTA), lateral patellofemoral angle (LPFA), thigh circumference reduction, Kujala score, and Lysholm score, were compared between two groups (i.e., active exercise and traditional exercise groups) preoperatively, 3 months postoperatively, 6 months postoperatively, 12 months postoperatively, and 24 months postoperatively.ResultsThe Kujala score was significantly higher in the active exercise group than traditional exercise group 3 months postoperatively (80.06 vs. 74.80, P &lt; 0.01), 6 months postoperatively (89.19 vs. 82.07, P &lt; 0.01), 12 months postoperatively (91.43 vs. 86.60, P &lt; 0.01), and 24 months postoperatively (92.50 vs. 90.27, P = 0.02). Similarly, there was a higher Lysholm score in the active exercise group compared with traditional exercise group 3 months postoperatively (81.25 vs. 76.53, P &lt; 0.01), 6 months postoperatively (89.81 vs. 84.80, P &lt; 0.01), 12 months postoperatively (93.25 vs. 88.40, P &lt; 0.01), and 24 months postoperatively (93.69 vs. 90.67, P &lt; 0.01). Significantly lower thigh circumference reduction was reported in the active exercise group compared with that in the traditional exercise group 3 months postoperatively (1.90 ± 0.57 vs. 2.45 ± 0.45, P &lt; 0.01) and 6 months postoperatively (1.50 ± 0.31 vs. 1.83 ± 0.32, P &lt; 0.01). No statistical difference was observed between the two groups in terms of PTA (P &gt; 0.05) or LPFA postoperatively (P &gt; 0.05).ConclusionsOur results suggested that active exercise therapy might benefit the early recovery of knee joint function and reduction of muscle atrophy in patients with recurrent patellar dislocation after MPFL reconstruction.
A Clinical Outcome of the Anti-PD-1 Therapy of Melanoma in Polish Patients Is Mediated by Population-Specific Gut Microbiome Composition
Authors: Pietrzak Bernadeta , Tomela Katarzyna , Olejnik-Schmidt Agnieszka , Galus Łukasz , Mackiewicz Jacek , Kaczmarek Mariusz , Mackiewicz Andrzej , Schmidt Marcin 
Published: November 2022

The gut microbiota is considered a key player modulating the efficacy of immune checkpoint inhibitor therapy. The study investigated the association between the response to anti-PD-1 therapy and the baseline gut microbiome in a Polish cohort of melanoma patients, alongside selected agents modifying the microbiome. Sixty-four melanoma patients enrolled for the anti-PD-1 therapy, and ten healthy subjects were recruited. The response to the treatment was assessed according to the response evaluation criteria in solid tumors, and patients were classified as responders or non-responders. The association between selected extrinsic factors and response was investigated using questionnaire-based analysis and the metataxonomics of the microbiota. In the responders, the Bacteroidota to Firmicutes ratio was higher, and the richness was decreased. The abundance of Prevotella copri and Bacteroides uniformis was related to the response, whereas the non-responders’ gut microbiota was enriched with Faecalibacterium prausnitzii and Desulfovibrio intestinalis and some unclassified Firmicutes. Dietary patterns, including plant, dairy, and fat consumption as well as gastrointestinal tract functioning were significantly associated with the therapeutic effects of the therapy. The specific gut microbiota along with diet were found to be associated with the response to the therapy in the population of melanoma patients.
Taxonomic Reassessment of Freshwater Mussels from the Western Balkans Reveals an Overlooked but Critical Refugium and Defines Conservation Priorities
Authors: Lyubas Artem A. , Kondakov Alexander V. , Tomilova Alena A. , Gofarov Mikhail Yu. , Eliseeva Tatyana A. , Konopleva Ekaterina S. , Vikhrev Ilya V. , Yunitsyna Olesya A. , Pešić Vladimir , Bolotov Ivan N. 
Published: November 2022

Freshwater mussels are an endangered group of animals, especially in the water bodies of Central and Western Europe. Conservation priorities were established for many endangered freshwater mussel species in the last decade. Here, we present new data on a cryptic refugium of freshwater mussels in the Western Balkans. Two species, Anodonta exulcerata Porro, 1838 and Unio elongatulus Pfeiffer, 1825, were discovered in Montenegro for the first time. The phylogenetic position and taxonomic status of freshwater mussel species from Montenegro were studied by means of an integrative approach, combining morphological data and COI gene sequences. Unio carneus Küster, 1854 stat. rev. represents a species with a restricted range, which is endemic to the Western Balkans. A viable population of Microcondylaea bonellii (Férussac, 1827) was discovered in the Zeta River. This species was not mentioned in Montenegro since the 1900s. A significant level of genetic diversity was revealed for the studied species. In the Skadar Lake basin, freshwater mussels from diverse ecological groups were discovered. Rheophilic species of freshwater mussels may represent ancient lineages, which need special conservation planning. Conservation priorities should be aimed at the protection of freshwater mussel habitats on the Balkan Peninsula.
Peran Kredibilitas Merek Memediasi Pengaruh Kredibilitas Endorser Terhadap Ekuitas Merek
Authors: Maulidasari Cut Devi , Damrus Damrus , Darmayanti Cici 
Published: November 2022

The purpose of this study was to examine the role of endorser credibility on brand equity. How to mediate brand credibility also supports this role. This study uses a qualitative approach by using secondary data in the form of literature and literature study. The results of this study show that brand credibility has actually become a mediation that can be used on endorser credibility and brand equity. This study states that an endorser must be someone who has been declared competent and credible, has attractiveness, ability or skill, and trust. The credibility of the endorser is believed to be able to develop the credibility of the brand.. Keywords: Brand Equity, Endorser, Brand Credibility, Celebrity
PENGARUH KEMITRAAN DAN INOVASI TERHADAP KEWIRAUSAHAAN SOSIAL DOSEN PTS BIDANG EKONOMI DI KOTA PEKANBARU
Authors: Hadiyati Hadiyati , Fatkhurahman Fatkhurahman 
Published: November 2022

Pelaksanaan tri dharma perguruan tinggi sebagai bentuk kewajiban dosen menjadi bagian penting, khusus bidang pengabdian masyarakat seorang dosen membutuhkan daya kreasi dalam menganalisis situasi permasalahan yang dihadapi masyarakat dan hal inilah dari masih dinilai belum optimal. Perguruan tinggi swasta di Kota Pekanbaru berjumlah 11 PTS dengan dosen sebanyak 284 orang, di tahun 2020 daftar penerima hibah pengabdian dikti hanya sebanyak 5 judul atau sebesar 1,76%. Kondisi ini mengindikasikan masih rendahnya kinerja dosen bidang pengabdian masyarakat pada PTS bidang ekonomi di Kota Pekanbaru yang membutuhkan kewirausahaan sosial yang tinggi. Diduga terdapat banyak faktor yang mempengaruhi kewirausahaan sosial dosen diantaranya adalah kemitraan dan inovasi. Metode yang digunakan adalah penelitian survei dengan pendekatan kuantitatif. Data dikumpulkan dari populasi dosen PTS bidang ekonomi sebanyak 284 orang dengan sampel diambil sebanyak 74 orang dengan teknik proporsional random sampling. Pengumpulan data dengan menggunakan kuesioner online dan teknik analisis data dengan menggunakan teknik analisis kuantitatif dengan alat regresi linier berganda. Hasil penelitian menunjukkan bahwa terbukti variabel kemitraan dan inovasi berpengaruh signifikan terhadap kewirausahaan sosial dosen PTS di Kota Pekanbaru dan juga diketahui variable inovasi memiliki pengaruh lebih besar dibandingkan kemitraan terhadap kewirausahaan social dosen