bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-Expression and Combined Prognostic Value of CSPG4 and PDL1 in TP53-Aberrant Triple-Negative Breast Cancer
Authors: Hu Zhe-Yu , Zheng Chanjuan , Yang Jianbo , Ding Siyu , Tian Can , Xie Ning , Xue Lian , Wu Muyao , Fu Shujun , Rao Zhouzhou , Price Matthew A. , McCarthy James B. , Ouyang Quchang , Lin Jizhen , Deng Xiyun 
Published: February 2022

BackgroundIn triple-negative breast cancer (TNBC), PDL1/PD1-directed immunotherapy is effective in less than 20% of patients. In our preliminary study, we have found CSPG4 to be highly expressed together with PDL1 in TNBCs, particularly those harboring TP53 aberrations. However, the clinical implications of co-expressed CSPG4 and PDL1 in TNBCs remain elusive.MethodsA total of 85 advanced TNBC patients treated in the Hunan Cancer Hospital between January 2017 and August 2019 were recruited. The expressions of CSPG4 and PDL1 in TNBC tissues were investigated using immunohistochemistry (IHC). The RNA-seq dataset from the TCGA-BRCA project was further used to analyze the mRNA expression of CSPG4 and PDL1 in TP53-aberrant TNBCs. Cox proportional hazards model and Kaplan–Meier curves with Logrank test was used to analyze the effects of CSPG4 and PDL1 on survival. TNBC cell lines were further used to investigate the molecular mechanism that were involved.ResultsTP53 aberrations occurred in more than 50% of metastatic TNBCs and were related to higher tumor mutation burden (TMB). In TCGA-BRCA RNA-seq dataset analysis, both CSPG4 and PDL1 levels were high in TNBCs, especially in TP53-aberrant TNBCs. IHC assay showed nearly 60% of advanced TNBCs to be CSPG4-positive and about 25% to be both CSPG4-positive and PDL1-positive. The levels of CSPG4 and PDL1 were high in TNBC cell lines as revealed by flow cytometry and immunoblotting compared with non-TNBC cells. Univariate Cox regression analysis indicated that CSPG4 positivity was a significant risk factor for progression-free survival in metastatic TNBCs, with a hazard ratio (HR) of 2.26 (P = 0.05). KM curves with Logrank test also identified high level of CSPG4 as a significant risk factor for overall survival in advanced breast cancers in TCGA-BRCA samples (P = 0.02). The immunoblotting assays showed that EMT-related pathways were involved in CSPG4-mediated invasion.ConclusionsCSPG4 expression level is associated with PDL1 positivity in TP53-aberrant TNBC cells. Patients with CSPG4 expression have poor treatment response and poor overall survival. Co-expressed CSPG4 and PDL1 may have an important prognostic value and provide new therapeutic targets in TNBC patients. CSPG4 might mediate tumor invasion and PDL1 overexpression through EMT-related pathway.
Mental Health Differences in Healthcare Workers Exposed to Different Risks During the Coronavirus Disease 2019 Pandemic
Authors: Zhang Yihui , Li Da , Ouyang Xuan , Bai Hanping , Zhao Linlin , Shi Yongyan , Tan Liwen 
Published: February 2022

BackgroundThe coronavirus disease 2019 (COVID-19) pandemic has caused a significant impact on the physical and mental health of healthcare workers. This study assessed the psychological status of healthcare workers who were exposed to different risk-levels in China and explored the factors that affected their mental health.MethodsDemographic, occupational characteristics, and mental health measurements were collected from 810 workers in 41 hospitals in China, through online questionnaires from February 11 to March 3, 2020. The degree of symptoms for fatigue, anxiety, and insomnia were assessed using the Chinese versions of the Fatigue Severity Scale, 7-item Generalized Anxiety Disorder Scale, and Insomnia Severity Index, respectively. Binary logistic regression analysis was performed to identify factors associated with mental health symptoms.ResultsAll 810 participants completed the relevant questionnaires without missing data. The prevalence of fatigue, anxiety, and insomnia symptoms was 74.3, 73.7, and 61.7%, respectively. Nurses, women, and workers exposed to high-risk areas were more likely to report mental health problems (P &lt; 0.05). After controlling for confounders, exposure to high-risk areas was independently associated with increased symptoms of fatigue, anxiety, and insomnia (fatigue among high-risk areas: OR, 3.87; 95% CI, 2.26–6.61; P &lt; 0.001; anxiety among high-risk areas: OR, 2.66; 95% CI, 1.58–4.51; P &lt; 0.001; insomnia among high-risk areas: OR, 2.83; 95% CI, 1.68–4.79, P &lt; 0.001).ConclusionThe study demonstrated significant differences in psychological symptoms among healthcare workers exposed to different levels of risk, and those in high-risk areas were more vulnerable to experiencing mental health symptoms. These findings emphasize the importance of giving due attention to healthcare workers, especially women, nurses, and those working in high-risk settings during the COVID-19 pandemic.
Effects of a 6 Week Yoga Intervention on Executive Functioning in Women Screening Positive for Adult ADHD: A Pilot Study
Authors: Fritz Kathryn , O'Connor Patrick J. 
Published: February 2022

PurposeLittle is known about the effects of yoga training in adults with ADHD symptoms. This pilot study sought to determine the feasibility and selected psychological effects of 6 weeks of yoga training in women screening positive for adult ADHD compared to a wait-list control group.MethodsA randomized trial was conducted with 32 adult women (18–24 years) who volunteered after screening positive for adult ADHD as assessed by the Adult ADHD Self-Report Scale (ASRS). Participants were randomized to 6 weeks of Bikram yoga training or to a wait-list control group. The yoga intervention consisted of two 90-min classes per week. Multilevel models were used to test hypothesized interactions of yoga-induced improvements compared to controls across time (baseline, 3 weeks, and 6 weeks). The primary outcomes assessed inhibitory control, cognitive flexibility and working memory using the NIH Toolbox. Separate models with trait mindfulness, trait anxiety and expectations for change in either attention or working memory as covariates tested whether these variables mediated the changes in the three measures of executive function. Secondary outcomes included mood, perceived functional impairment and motivation for, and hyperactivity during, the cognitive tests.ResultsNo adverse events were observed. Attendance averaged 91.7% among the 69% of the sample that did not dropout. No significant Group X Time interactions were found for any of the psychological outcomes and the null executive function findings were unchanged when including the covariates.ConclusionSix-weeks of yoga training twice per week is potentially feasible for women experiencing ADHD symptoms, but an exercise stimulus of this duration and magnitude yields no beneficial cognitive or mood outcomes.
Laparoscopic Radical Resection of Colorectal Cancer in the Treatment of Elderly Colorectal Cancer and Its Effect on Gastrointestinal Function
Authors: Liu Biao , Yao Chuanhui , Li Haiying 
Published: February 2022

ObjectiveTo explore the efficacy and safety of laparoscopic radical resection of colorectal cancer in the elderly patients and its impact on gastrointestinal function.MethodsA total of 122 elderly patients with colorectal cancer admitted to our hospital from March 2020 to June 2021 were selected as the research subjects, and they were divided into the control group (n = 61) and the observation group (n = 61). The control group was treated with traditional laparotomy, and the observation group was treated with laparoscopic radical resection of colorectal cancer. The clinical data of operation time, incision length, intraoperative bleeding volume, and hospitalization time in the two groups were recorded. Serum motilin (MTL) and gastrin (GAS) levels were measured pre- and post-operatively. The duration of abdominal distension, the time for the abdominal sound to return to normal, the time for the anal exhaust to normal, and the time for normal food intake were recorded after operation. The patients were followed up for 6 months post-operatively, and the complications during follow-up were recorded.ResultsThe total response rate of the observation group (95.08%) was higher than that of the control group (81.97%) (P &lt; 0.05). The operation time, incision length, intraoperative bleeding volume, and hospitalization time of the observation group were lower than those of the control group (P &lt; 0.05). The duration of abdominal distension, the time for bowel sounds to return to normal, the time for the anus to exhaust gas to normal, and the normal eating time in the observation group were all lower than those in the control group (P &lt; 0.05). After surgery, the levels of MTL and GAS in the two groups were lower than those before surgery, and those in the observation group were lower than those in the control group (P &lt; 0.05). The total incidence of complications in the observation group (3.28%) was lower than that in the control group (13.12%) (P &lt; 0.05).ConclusionLaparoscopic radical resection of colorectal cancer in the elderly patients has good effect, short operation time, less trauma, less blood loss during operation, short hospital stay, good recovery of gastrointestinal function, fewer complications, and high safety.
The Impact of Accelerated HF-rTMS on Canine Brain Metabolism: An [18F]-FDG PET Study in Healthy Beagles
Authors: Xu Yangfeng , Peremans Kathelijne , Courtyn Jan , Audenaert Kurt , Dobbeleir Andre , D'Asseler Yves , Achten Eric , Saunders Jimmy , Baeken Chris 
Published: February 2022

BackgroundRepetitive transcranial magnetic stimulation (rTMS) has been proven to be a useful tool for the treatment of several severe neuropsychiatric disorders. Accelerated (a)rTMS protocols may have the potential to result in faster clinical improvements, but the effects of such accelerated paradigms on brain function remain to be elucidated.ObjectivesThis sham-controlled arTMS study aimed to evaluate the immediate and delayed effects of accelerated high frequency rTMS (aHF-rTMS) on glucose metabolism in healthy beagle dogs when applied over the left frontal cortex.MethodsTwenty-four dogs were randomly divided into four unequal groups: five active (n = 8)/ sham (n = 4) stimulation sessions (five sessions in 1 day), 20 active (n = 8)/ sham (n = 4) stimulation sessions (five sessions/ day for 4 days), respectively. [18F] FDG PET scans were obtained at baseline, 24 h poststimulation, after 1 and 3 months post the last stimulation session. We explicitly focused on four predefined regions of interest (left/right prefrontal cortex and left/right hippocampus).ResultsOne day of active aHF-rTMS- and not sham- significantly increased glucose metabolism 24 h post-active stimulation in the left frontal cortex only. Four days of active aHF-rTMS only resulted in a nearly significant metabolic decrease in the left hippocampus after 1 month.ConclusionsLike in human psychiatric disorders, active aHF-rTMS in healthy beagles modifies glucose metabolism, although differently immediately or after 1 month post stimulation. aHF-rTMS may be also a valid option to treat mentally disordered dogs.
Dry Eye Syndrome and Therapeutic Efficacy of the Tear Substitute HYLO-PARIN
Authors: Чекина А.Ю. , Марченко Л.Н. , Джумова М.Ф. , Далидович А.А. , Качан Т.В. , Терешенко О.В. 
Published: February 2022

Оценить терапевтическую эффективность слезозаменителя ХИЛО-ПАРИН при амбулаторном лечении пациентов с воспалительными заболеваниями глазной поверхности, протекающими на фоне синдрома «сухого глаза» (ССГ). Представлены результаты амбулаторного лечения 32 пациентов (64 глаза) с различной степенью тяжести ССГ и клиническими признаками блефароконъюнктивита, кератита. Описаны причины и клинические проявления ССГ. Диагноз устанавливали на основании биомикроскопии глазной поверхности. Обращали внимание на прокрашивание эпителия роговицы и конъюнктивы флюоресцеином; оценивали степень гиперемии конъюнктивы и выраженность складок конъюнктивы; оценивали состояние выводных протоков мейбомиевых желез; стабильность прероговичной слезной пленки; величину суммарной слезопродукции. Продолжительность лечения пациентов составляла от 1 до 2 месяцев, частота инстилляций ХИЛО-ПАРИН – от 4 до 6 раз в день.Результаты применения препарата ХИЛО-ПАРИН продемонстрировали его терапевтическую эффективность при лечении пациентов с различными проявлениями кератита и блефароконъюнктивита на фоне синдрома «сухого глаза».
To evaluate the therapeutic efficacy of HYLO-PARIN tear replacer in the outpatient treatment of patients with inflammatory-dystrophic ocular surface disease with concomitant "dry eye" syndrome. The causes and clinical manifestations of "dry eye" syndrome are described. The results of outpatient treatment of 32 patients (64 eyes) with varying degrees of severity of dry eye syndrome are presented. The treatment duration ranged from 1 to 2 months, the frequency of HYLO-PARIN instillation – from 4 to 6 times a day.The HYLO-PARIN eye drops application demonstrated therapeutic efficacy in patients with various keratoconjunctivitis manifestations on the background of the "dry eye" syndrome.
Cerebral Vasculitis Caused by Mixed Viral Infection as a Cause of Ischemic Stroke in a Child
Authors: Куликова С.Л. , Лихачев С.А. , Левшук О.Н. , Кишкурно Е.П. , Дивакова Е.В. , Дмитриев В.В. 
Published: February 2022

Наиболее частой причиной инфаркта мозга в детском возрасте является церебральная артериопатия, возникающая в силу различных механизмов. Одной из причин вторичных церебральных васкулитов у детей являются недавно перенесенные либо активные вирусные инфекции.В статье представлено описание клинического случая ребенка 5 лет, у которого развились повторные инфаркты мозга в вертебробазилярном бассейне вследствие церебрального васкулита. Исключены наследственные тромбофилии, антифосфолипидный синдром, системные васкулиты, Лайм-боррелиоз, COVID-19-инфекция. При исследовании сыворотки крови методом ПЦР на вирусы группы герпеса и энтеровирусы (на 17-й день болезни) обнаружена ДНК ВЭБ (1 203 копий/мл), ВГЧ-6В (56 копий/мл) и ВГЧ-7. Также отмечено повышение уровня гомоцистеина легкой степени.Наиболее очевидной причиной развития церебрального васкулита у данного ребенка явилась активно протекающая микст-вирусная инфекция (ВГЧ-6B + ВГЧ-7 + ВЭБ), которая привела к локальному воспалению сосудистой стенки церебральных сосудов, изменению церебральной гемодинамики. Применение антиагрегантной и антикоагулянтной терапии в сочетании с противовирусной (ганцикловир) и иммуномодулирующей (кортикостероиды системные, иммуноглобулин человеческий нормальный) позволило избежать повторных эпизодов нарушения мозгового кровообращения.
The most common cause of cerebral infarction in childhood is cerebral arteriopathy due to various mechanisms. One of the causes of the secondary cerebral vasculitis in children is recent or active viral infections.The article describes a clinical case of a 5-year-old child, who developed repeated cerebral infarctions in the posterior circulation system due to cerebral vasculitis. Hereditary thrombophilia, antiphospholipid syndrome, systemic vasculitis, Lyme disease, COVID-19 infection are excluded. In the study of blood serum by PCR for viruses of the herpes group and enterovirus (on the 17th day of disease), EBV DNA (1,203 copies / ml), HHV-6В (56 copies / ml), and HHV-7 were detected. There was also a slight increase of homocysteine level.The most obvious cause of development of cerebral vasculitis in this child was an active mixed viral infection (HHV-6B + HHV-7 + EBV), which led to local inflammation of the vascular wall of cerebral vessels and changes in cerebral hemodynamics. The use of antiplatelet and anticoagulant therapy in combination with antiviral (ganciclovir) and systemic corticosteroids, intravenous immune globulin (IVIG) allowed to avoid repeated episodes of cerebrovascular accident.
Effectiveness of Hypobaric Adaptation and Cellular Chronoblockers for Prevention of the Development of the Falls Syndrome in the Concept of Preventive Geriatrics
Authors: Оленская Т.Л. 
Published: February 2022

В связи с постарением населения актуальным является поиск компонентов профилактики развития и прогрессирования основных гериатрических синдромов, в частности синдрома падений, у лиц среднего возраста с точки зрения превентивной гериатрии на амбулаторном и домашнем этапах медицинской реабилитации.Целью работы явилось изучение эффективности гипобарической адаптации и клеточных хроноблокаторов для профилактики развития синдрома падений у пациентов среднего возраста на амбулаторном и домашнем этапах медицинской реабилитации.В работе методом стабилометрии изучена эффективность применения курса гипобарической барокамерной адаптации, клеточных хроноблокаторов для повышения статокинетической устойчивости у пациентов среднего возраста.Полученные результаты позволяют рекомендовать предложенные компоненты для практического применения в концепции Национальной стратегии «Активное долголетие – 2030» и в программах медицинской реабилитации на амбулаторном и домашнем этапах.
In connection with aging of the population, it is urgent to search for the components of prevention of the development and progression of major geriatric syndromes, in particular the fall syndrome, in middle-aged people from the point of view of preventive geriatrics at the outpatient and home stages of medical rehabilitation.The aim of the work was to study the effectiveness of hypobaric adaptation and cellular chronoblockers for prevention of the development of the fall syndrome in middle-aged patients at the outpatient and home stages of medical rehabilitation.In the work, the method of stabilometry was used to study the effectiveness of the course of hypobaric pressure chamber adaptation, cellular chronoblockers to increase the statokinetic stability of middle-aged patients.The obtained results allow us to recommend the proposed components for practical application in the concept of the National Strategy “Active Longevity – 2030” and in medical rehabilitation programs at the outpatient and home stages.
Components of Medical Rehabilitation at the Outpatient and Home Stages of Older People Suffering after COVID-19 Pneumonia
Authors: Оленская Т.Л. , Николаева А.Г. , Азаренок М.К. , Юхно Ю.С. , Шишко О.И. 
Published: February 2022

Появление и распространение инфекции COVID-19 поставило перед специалистами здравоохранения задачи, связанные с оказанием программ восстановления и реабилитации для пациентов разных возрастных групп после перенесенных пневмоний.Для лиц старшего возраста после перенесенных пневмоний, ассоциированных с инфекцией COVID-19, разработан инновационный YouTube-проект «Поможем друг другу восстановиться после перенесенной пневмонии».В работе представлены результаты обследования лиц старшего возраста после перенесенной пневмонии, ассоциированной с инфекцией COVID-19, до и после одно- и двухкомпонентных программ медицинской реабилитации.Показана рациональность применения для пациентов пожилого возраста на амбулаторном и домашнем этапах медицинской реабилитации курса Цитофлавина, предложенной методики гипобарической барокамерной адаптации и их сочетания, а также кинезиотейпирования заднего свода диафрагмы и психотерапевтической коррекции.
The emergence and spread of COVID-19 infection has set health professionals the task of providing recovery and rehabilitation programs for patients of different age groups after suffering from pneumonia.For older people who have suffered from pneumonia associated with COVID-19 infection, an innovative component of the remote online form of work, the YouTube project "Let's help each other recover from pneumonia", has been developed.The paper presents the results of a survey of older people after pneumonia associated with COVID-19 infection before and after one-and two-component medical rehabilitation programs.The rationality of the use of a course of Cytoflavin for elderly patients at the outpatient and home stages of medical rehabilitation, the proposed method of adaptation of the hypobaric pressure chamber and their combination, as well as kinesiotaping of the posterior arch and psychotherapeutic correction is shown.
Mythical Diagnoses in Pediatrics: Adenoiditis or Nasopharyngitis? Which diagnosis is correct? Questions and Answers
Authors: Попович В.И. , Бекетова Г.В. 
Published: February 2022

В педиатрии существует много так называемых мифических диагнозов, среди которых и «аденоидит», который часто используется врачами первого контакта. В статье в форме вопросов и ответов представлена мультидисциплинарная дискуссия, касающаяся анатомо-физиологических подходов в формировании диагноза «острый риносинусит» и «острый назофарингит». Авторы обсуждают врачебные ошибки в отношении синдрома насморка у детей, неправомерности диагноза «аденоидит», понятия «аденоидные вегетации», физиологической роли лимфоидной ткани в носоглотке. Представлены клинические критерии острого риносинусита и острого назофарингита, их этиология и патогенетическая сущность. Рассмотрены современные подходы к лечению поражений носа, околоназальных пазух и носоглотки с использованием стандартизованных фитониринговых препаратов Синупрет и Имупрет.
In pediatrics, there are many so-called mythical diagnoses, including “adenoiditis”, which is often used by doctors of the first contact. The article in the form of “questions and answers” presents a multidisciplinary discussion concerning the anatomical and physiological approaches in the formation of the diagnoses “acute rhinosinusitis” and “acute nasopharyngitis.” The authors discuss medical errors in relation to the rhinitis syndrome in children, inappropriateness of the diagnosis of “adenoiditis”, the concept of “adenoid vegetation”, physiological role of lymphoid tissue in the nasopharynx. The clinical criteria for acute rhinosinusitis and acute nasopharyngitis, their etiology and pathogenetic essence are presented. Modern approaches to the treatment of lesions of the nose, paranasal sinuses, and nasopharynx using the standardized phytoneering drugs Sinupret and Imupret are considered.
PERAN ANTIOKSIDAN DALAM MENGATASI CEKAMAN KEKERINGAN PADA TANAMAN KEDELAI (Glycine max (L.) Merril)
Authors: Asyura AG Laily 
Published: February 2022

The decreasing of soybean production full fill of soybean in Indonesia, one of the main causes is the decreasing of land farming area. To increase the production of soybean in Indonesia can be reached by the expansion of planting areas,use potential land. This research has held in plastic house Agriculture Faculty, University of Sumatera Utara, Medan on altitude +32 meters above sea level, to August 2016 until November 2016,using factorial rendomized block design with 2 factors.The first factor is drought stress with 3 levelsof KL 80% ; KL 60% ; KL 40% and the second factor is apllication antioxidants with 2 kind of Ascorbic Acid and Salicylic Acid.The result of this research showed that drought stresswere significantly effect to leaf area, the number of pods fullness per plant, dry weight of seeds per plant and dry weight of 100 seeds. Apllication antioxidantswere significantly effect to chlorophyll a, chlorophyll b, thenumber of pods fullness per plant, dry weight of seeds per plant and dry weight of 100 seeds.Interaction of drought stress and application antioxidant were significantly effect to dry weight of seeds per plant and dry weight of 100 seeds.