bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translation and psychometric testing of the simplified version of the health-related diet and exercise self-efficacy scale in China
Authors: Mao Xia , Zeng Xuefang , Zhong Qinyi , Guo Jia 
Published: August 2022

Abstract
Background
Measuring health-related diet and exercise self-efficacy is an important first step in improving healthy behaviors and health outcomes. However, we did not find a self-efficacy measurement in Chinese that is specifically targeted at diet and exercise self-efficacy among healthy adults.

Aim
The present study aimed to translate the Health-Related Diet and Exercise Self-Efficacy Scale -simplified version into Mandarin Chinese (HRDESES) and evaluate its reliability and validity in Chinese healthy adults.

Methods
The HRDESES was translated and adapted to the Chinese context, with a good content validity of 0.86 among seven experts. The survey was then carried out in 216 adults in Hunan, China. Testing of the reliability included internal consistency reliability and test-retest reliability, while validity included content validity, construct validity, and criterion validity.

Results
The Cronbach's α of the HRDESES was 0.87 for the total scale, 0.86 for the diet subscale and 0.91 for the exercise subscale; the McDonald's ω of the HRDESES-SC was 0.85 for the total scale, 0.86 for the diet subscale and 0.91 for the exercise subscale, all demonstrating good internal consistency. The test-retest reliability was 0.88 for the total scale, 0.81 for the diet subscale and 0.82 for the exercise subscale, demonstrating good test-retest reliability. For construct validity, the scale effectively distinguished subjects by age, gender, education, occupation, marital status, and family income, showing good discriminant validity. The confirmatory factor analysis (CFA) supported a two-factor structure of the scale: diet and exercise subscale. It was demonstrated that the HRDESES was highly associated with the General Self-Efficacy Scale and its two subscales, with correlation coefficients ranging from 0.83 to 0.86 (p < 0.05), showing high criterion validity.

Conclusion
The HRDESES had good reliability and validity and could be used as a simple and effective tool for assessing the health-related diet and exercise self-efficacy in Chinese healthy adults.
Total laparoscopic pancreaticoduodenectomy with left posterior superior mesenteric artery first-approach and plexus-preserving circumferential lymphadenectomy: step-by-step technique with a surgical case report (with video)
Authors: Khiem Thanh , Hoi Ham , Hiep Tuan , Khue Kim , Duy Van , Inoue Yosuke , Son Hong , Dung Duc 
Published: August 2022

Abstract
Introduction
Total laparoscopic pancreaticoduodenectomy (tLPD) for cancer of the Vater remains a challenging procedure. Recently, several meta-analyses showed the superior aspects of “superior mesenteric artery (SMA)-first approach,” “systematic mesopancreas dissection,” and “circumferential lymphadenectomy around SMA” in increasing R0 resection rate and reducing postoperative complications including pancreatic fistula and bleeding as well as improving overall survival particularly.

Case presentation
Our patient is a 70-year-old female with a no special medical history, recruited because of jaundice. She was referred for pancreaticoduodenectomy because of a 10-mm-sized mass in distal bile duct referred to as Vater’s tumor. We used 5 trocars, and the patient was placed in a Trendelenburg position. The transverse colon was lifted, the first loop of the jejunum was pulled to the left, and lymph node groups 14th and 15th were removed en bloc and then exposed the SMA from the anterior to the left posterior side from the caudal side to the origin. The first jejunal vessels and the posterior inferior pancreaticoduodenal artery were ligated as well as the extensive mobility of the duodenum and head of the pancreas from the left side. The systematic mesopancreas dissection from the right site of the SMA will be easily and conveniently done afterwards. Histopathological examination of ypT2N1 indicated that 1 of the 22 lymph nodes was positive, which was 1 of 7 LN no. 14. Pathological results showed a Vater adenocarcinoma with all margins being negative.

Conclusions
This technique was safe and effective to perform precise level 2 mesopancreas dissection and complete lymphadenectomy around SMA without dissection of pl-SMA in laparoscopic field.
An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster
Authors: Fiol Arnau , Jurado-Ruiz Federico , López‑Girona Elena , Aranzana Maria José 
Published: August 2022

Abstract
Background
Genome complexity is largely linked to diversification and crop innovation. Examples of regions with duplicated genes with relevant roles in agricultural traits are found in many crops. In both duplicated and non-duplicated genes, much of the variability in agronomic traits is caused by large as well as small and middle scale structural variants (SVs), which highlights the relevance of the identification and characterization of complex variability between genomes for plant breeding.

Results
Here we improve and demonstrate the use of CRISPR-Cas9 enrichment combined with long-read sequencing technology to resolve the MYB10 region in the linkage group 3 (LG3) of Japanese plum (Prunus salicina). This region, which has a length from 90 to 271 kb according to the P. salicina genomes available, is associated with fruit color variability in Prunus species. We demonstrate the high complexity of this region, with homology levels between Japanese plum varieties comparable to those between Prunus species. We cleaved MYB10 genes in five plum varieties using the Cas9 enzyme guided by a pool of crRNAs. The barcoded fragments were then pooled and sequenced in a single MinION Oxford Nanopore Technologies (ONT) run, yielding 194 Mb of sequence. The enrichment was confirmed by aligning the long reads to the plum reference genomes, with a mean read on-target value of 4.5% and a depth per sample of 11.9x. From the alignment, 3261 SNPs and 287 SVs were called and phased. A de novo assembly was constructed for each variety, which also allowed detection, at the haplotype level, of the variability in this region.

Conclusions
CRISPR-Cas9 enrichment is a versatile and powerful tool for long-read targeted sequencing even on highly duplicated and/or polymorphic genomic regions, being especially useful when a reference genome is not available. Potential uses of this methodology as well as its limitations are further discussed.
Acute kidney injury-associated delirium: a review of clinical and pathophysiological mechanisms
Authors: Pang Haoming , Kumar Sanjeev , Ely E. Wesley , Gezalian Michael M. , Lahiri Shouri 
Published: August 2022

AbstractAcute kidney injury is a known clinical risk factor for delirium, an acute cognitive dysfunction that is commonly encountered in the critically ill population. In this comprehensive review of clinical and basic research studies, we detail the epidemiology, clinical implications, pathogenesis, and management strategies of patients with acute kidney injury-associated delirium. Specifically addressed are the pathological roles of endogenous toxin or drug accumulation, acute kidney injury-mediated neuroinflammation, and acute kidney injury-associated volume overload as discrete potential biological mechanisms of the condition. The optimization of clinical contributors and normalization of renal function are reviewed as pragmatic management strategies in addition to potential and emerging therapeutic approaches.
In vitro total phenolics, total flavonoids, antioxidant and antibacterial activities of selected medicinal plants using different solvent systems
Authors: Mehmood Ansar , Javid Sonia , Khan Muhammad Faraz , Ahmad Khawaja Shafique , Mustafa Amna 
Published: August 2022

AbstractRecently, an interest has surged in utilizing indigenous medicinal plants to treat infectious illnesses and extract bioactive substances, highlighting the need to analyze medicinal plants for phytochemicals and bioactivities. The present study was aimed to evaluate the impact of different solvent systems (aqueous, ethanol, and methanol) used for extraction on total phenolics, total flavonoids, antioxidant, and antibacterial activity of three medicinal plants of Azad Kashmir (Achillea millefolium,Bergenia ciliata,andAloe vera). High phenolic content was found in methanol extracts ofB. ciliata(27.48 ± 0.58 mg GAE/g dry weight),A. vera(25.61 ± 0.33 mg GAE/g dry weight), andA. millefolium(24.25 ± 0.67 mg GAE/g dry weight). High flavonoid content was obtained in the ethanol extract ofA. millefolium(27.13 ± 0.64 mg QE/g dry weight), methanol extract ofB. ciliata(17.44 ± 0.44 ± 0.44 mg QE/g dry weight), and the methanol extract ofA. vera(14.68 ± 0.67 mg QE/g dry weight). Strong 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) was obtained with a methanol extract ofB. ciliata(IC50 = 60.27 ± 0.20 µg/mL). With a zone of inhibition and a minimum inhibitory concentration ranging from 10.00 ± 0.66 to 24.67 ± 1.21 mm and 78 to 625 µg/mL, respectively, all of the studied plants demonstrated notable antibacterial activity againstStaphylococcus aureusandEscherichia coli.A. verashowed greater antibacterial activity as compared to other plants under study while methanolic extract showed greater antibacterial activity than ethanolic and aqueous extract. The findings of this research support the use of these medicinal plants to treat a variety of diseases.
Synopsis of the ticks of Algeria with new hosts and localities records
Authors: Mechouk Noureddine , Mihalca Andrei Daniel , Deak Georgiana , Bouslama Zihad 
Published: August 2022

Abstract
Background
Ticks are obligate hematophagous arthropods with a world-wide distribution that are extremely important not only in terms of human and animal health but also economically. In Algeria, information on tick species is scarce.

Methods
A systematic literature review was performed using online databases. The information extracted from the databases was was supplemented by information from an original study. Ticks were collected from various hosts and by flagging from January 2018 to December 2019.

Results
To date, in Algeria a total of 36 valid tick species belonging to two families have been recorded: (1) family Argasidae, with three Argas species and nine Ornithodoros species recorded; and (ii) family Ixodidae, with one Dermacentor species, three Haemaphysalis species, 10 Hyalomma species, four Ixodes species and six Rhipicephalus species recorded. The geographical distribution for each species was determined and listed. Eight new tick-host associations were recorded: four for Ixodes inopinatus sensu Estrada-Peña et al. 2014, one for Rhipicephalus bursa, one for R. turanicus, one for Hyalomma marginatum and one for Hy. lusitanicum. To our best knowledge, this study is the first to report the presence of I. inopinatus sensu Estrada-Peña et al. 2014 in Algeria. We also report here for the first time all tick species (Argasidae and Ixodidae) known to be present in Algeria.

Conclusion
This article represents a tool for students and scientists who work in the field of ticks and provides important new data on the distribution of ticks in Algeria.

Graphical Abstract
Placental imprinting of SLC22A3 in the IGF2R imprinted domain is conserved in therian mammals
Authors: Ishihara Teruhito , Griffith Oliver W. , Suzuki Shunsuke , Renfree Marilyn B. 
Published: August 2022

Abstract
Background
The eutherian IGF2R imprinted domain is regulated by an antisense long non-coding RNA, Airn, which is expressed from a differentially methylated region (DMR) in mice. Airn silences two neighbouring genes, Solute carrier family 22 member 2 (Slc22a2) and Slc22a3, to establish the Igf2r imprinted domain in the mouse placenta. Marsupials also have an antisense non-coding RNA, ALID, expressed from a DMR, although the exact function of ALID is currently unknown. The eutherian IGF2R DMR is located in intron 2, while the marsupial IGF2R DMR is located in intron 12, but it is not yet known whether the adjacent genes SLC22A2 and/or SLC22A3 are also imprinted in the marsupial lineage. In this study, the imprinting status of marsupial SLC22A2 and SLC22A3 in the IGF2R imprinted domain in the chorio-vitelline placenta was examined in a marsupial, the tammar wallaby.

Results
In the tammar placenta, SLC22A3 but not SLC22A2 was imprinted. Tammar SLC22A3 imprinting was evident in placental tissues but not in the other tissues examined in this study. A putative promoter of SLC22A3 lacked DNA methylation, suggesting that this gene is not directly silenced by a DMR on its promoter as seen in the mouse. Based on immunofluorescence, we confirmed that the tammar SLC22A3 is localised in the endodermal cell layer of the tammar placenta where nutrient trafficking occurs.

Conclusions
Since SLC22A3 is imprinted in the tammar placenta, we conclude that this placental imprinting of SLC22A3 has been positively selected after the marsupial and eutherian split because of the differences in the DMR location. Since SLC22A3 is known to act as a transporter molecule for nutrient transfer in the eutherian placenta, we suggest it was strongly selected to control the balance between supply and demand of nutrients in marsupial as it does in eutherian placentas.
Isolated posterior ST-elevation myocardial infarction: the necessity of routine 15-lead electrocardiography: a case series
Authors: Alsagaff Mochamad Yusuf , Amalia Rizki , Dharmadjati Budi Baktijasa , Appelman Yolande 
Published: August 2022

AbstractBackgroundTrue isolated posterior myocardial infarction is an uncommon finding of acute coronary syndrome, with an incidence rate of 3–7%. The prevalence rates of isolated posterior myocardial infarction in men and women are 72% and 28%, respectively. This uncommon finding may be attributed to multiple factors, such as unremarkable changes on 12-lead electrocardiography, a lack of awareness or knowledge, and an absence of diagnostic consensus, which leads to reperfusion delay and poor clinical outcomes.Case summaryHerein, we report three cases of acute myocardial infarction presenting as isolated ST-segment elevation in the posterior leads (V7–V9): Asian men aged 57, 62, and 53 years, who presented with ST-segment depression in V1–V3 that resolved gradually. Coronary angiography revealed a total/critical occlusion of the proximal circumflex coronary artery in all three cases. Routine and accurate interpretations of 15-lead electrocardiography (12-lead with additional V7–V9) resulted in a better sensitivity for isolated posterior myocardial infarction diagnoses, followed by a timely and opportune primary percutaneous coronary intervention.ConclusionsIsolated posterior myocardial infarction is a rare but potentially fatal event that is often accompanied by atypical and subtle changes on 12-lead electrocardiography (especially in the V1–V3 precordial leads) and may remain undetected by physicians. Therefore, the comprehensive and routine application of posterior leads is a crucial addition to the standard diagnosis and management of acute coronary syndrome in patients with subtle ST-segment changes who do not fulfill the criteria for ST-elevation myocardial infarction.
Mastopexy using de-epithelialised dermoglandular flaps: a case series for maximal volume conservation following breast implants removal
Authors: Khan Umar Daraz , Naseem Salma , Rafiq Sadia 
Published: August 2022

Abstract
Aims and objective
Removal of implants without replacement is often requested, and the procedure is more commonly performed today than ever before. However, the resultant loss of body image, secondary to the loss of breast volume, is not an outcome, that a patient is looking forward to. There is a lack of information on the options available to the patients following explantation. This case series presents an option of breast volume preservation and reshaping during mastopexy after breast implant removal that can be offered to selected patients. In the current case series, de-epithelialised dermoglandular flap mastopexy was used as an autologous tissue for breast reshaping and remodelling.

Material and methods
Since 2015, ten patients were selected for de-epithelialised dermoglandular mastopexy using wise pattern or vertical scar. Surgery was performed under general anaesthesia as a day case. A vertically oriented bipedicular dermoglandular flap was used for vertical scar mastopexy in two patients, and eight patients had Wise pattern incisions. Of these eight patients, four had superomedial and four had inferiorly based flaps for dermoglandular mastopexy and closure. All patients had a preoperative cup size D or larger.

Results
All patients had adequate results with an acceptable breast cup size. There was no skin breakdown, nipple loss, haematoma or infection.

Conclusion
De-epithelialised dermoglandular flap mastopexy is a safe procedure and can be used as an option in selected patients.

Level of Evidence
IV.
Evaluating the effects of analytical decisions in large-scale assessments: analyzing PISA mathematics 2003-2012
Authors: Heine Jörg-Henrik , Robitzsch Alexander 
Published: August 2022

AbstractResearch questionThis paper examines the overarching question of to what extent different analytic choices may influence the inference about country-specific cross-sectional and trend estimates in international large-scale assessments. We take data from the assessment of PISA mathematics proficiency from the four rounds from 2003 to 2012 as a case study.MethodsIn particular, four key methodological factors are considered as analytical choices in the rescaling and analysis of the data: (1) The selection of country sub-samples for item calibration differing at three factor levels. (2) The item sample refering to two sets of mathematics items used within PISA. (3) The estimation method used for item calibration: marginal maximum likelihood estimation method as implemented in R package TAM or an pairwise row averaging approach as implemented in the R package pairwise. (4) The type of linking method: concurrent calibration or separate calibration with successive chain linking.FindingsIt turned out that analytical decisions for scaling did affect the PISA outcomes. The factors of choosing different calibration samples, estimation method and linking method tend to show only small effects on the country-specific cross-sectional and trend estimates. However, the selection of different link items seems to have a decisive influence on country ranking and development trends between and within countries.
Scientific policies of developing countries and mid-level entry strategy: from the perspective of a marine scientist
Authors: Lee Sang-Mook 
Published: August 2022

AbstractScientific developments are considered the primary source of the economic prosperity of a country. This faith in science appears stronger among economists and policymakers than those involved in day-to-day activities. However, science policy is often confused with a policy for science. The former is dictated by national interest and ideology, including long-term plans for the country. Because it is so crucial, whether to let scientists formulate the science policy of a nation is a long-standing issue in many countries. Policymakers and politicians generally provide the overall picture with some help from scientists. A common approach adopted by developing countries is the so-called ‘mid-level entry strategy.’ The idea is to dispense basic science to developed countries and focus on those areas that may bring immense profits. And when the time comes, and technology matures, bet heavily on those specific areas and jump ahead of the flock to reap the benefit. However, many consider DARPA (Defense Advanced Research Projects Agency) the most successful research and development (R&D) institution in the US. It is said that the accomplishment of the agency was possible because they started with basic science and moved up to applied and practical research. Doing so dominated the entire value chain, and the know-how was then disseminated to the public. Such observation leads one to conclude that, contrary to the myth of mid-level entry strategy, the real big reward for R&D of a country comes from starting at the fundamental level. On the other hand, because scientific and technological development is not straightforward, some examples may point to the contrary.
Level of asthma control and its determinants among adults living with asthma attending selected public hospitals in northwestern, Ethiopia: using an ordinal logistic regression model
Authors: Belachew Eyayaw Ashete , Tadess Sumeya , Alemayehu Mekuriaw , Ayele Emneteab Mesfin 
Published: August 2022

Abstract
Background
Asthma is a major public health challenge and is characterized by recurrent attacks of breathlessness and wheezing that vary in severity and frequency from person to person. Asthma control is an important measure of health outcomes of the patients with asthma and reflecting the impact of an illness and its treatment from the patient’s perspective. Therefore, this study assessed the asthma control levels and their determinants among adults living with asthma in selected public referral hospitals in northwestern Ethiopia.

Materials and method
A multicenter institutional-based cross-sectional study was conducted in North-western Ethiopia, from October to December 2021. A systematic random sampling technique was employed to recruit the study participants. Bi-variable and multivariable ordinal logistic regression was used to determine the independent predictors of asthma control levels. A p-value of < 0.05 was considered as statistically significant.

Result
A total of 409 patients were included in the final analysis. Asthma was controlled by 28.9% with 95%CI (24.7, 33.5) people who have asthma. Regarding the potential predictor of asthma control level, being male (AOR = 6.5, 95%CI (1.28, 32.44), Married (AOR = 3.62, 95%CI (1.28, 10.27), healthcare provider adherence to guideline usage (AOR = 8.4,95%CI (2.7, 26) and non-fuel users (AOR = 6.0, 95%CI (1.5, 22.5) were variables that increase asthma control. However, non-adherent to medication (AOR = 0.16, 95%CI (0.059, 0.48), low level of patient enablement (AOR = 0.19, (95%CI) (0.08, 0.49) and poor relationship with healthcare provider (AOR = 0.024,95%CI (0.02, 0.23) were variables that significantly decreased asthma control level.

Conclusion
The findings indicated that asthma control remains suboptimal in a large proportion of patients with asthma in the study setting. Socio-demographic, clinical, healthcare-related, and medication-related variables were significantly associated with asthma control. Therefore, our study highlights multifaceted interventions, including comprehensive asthma education along with an integrated treatment plan to improve asthma control and quality of life.