bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-expression network analysis of frontal cortex during the progression of Alzheimer’s disease
Authors: Beck John S , Madaj Zachary , Cheema Calvin T , Kara Betul , Bennett David A , Schneider Julie A , Gordon Marcia N , Ginsberg Stephen D , Mufson Elliott J , Counts Scott E 
Published: January 2022

AbstractMechanisms of Alzheimer’s disease (AD) and its putative prodromal stage, amnestic mild cognitive impairment (aMCI), involve the dysregulation of multiple candidate molecular pathways that drive selective cellular vulnerability in cognitive brain regions. However, the spatiotemporal overlap of markers for pathway dysregulation in different brain regions and cell types presents a challenge for pinpointing causal versus epiphenomenal changes characterizing disease progression. To approach this problem, we performed Weighted Gene Co-expression Network Analysis and STRING interactome analysis of gene expression patterns quantified in frontal cortex samples (Brodmann area 10) from subjects who died with a clinical diagnosis of no cognitive impairment, aMCI, or mild/moderate AD. Frontal cortex was chosen due to the relatively protracted involvement of this region in AD, which might reveal pathways associated with disease onset. A co-expressed network correlating with clinical diagnosis was functionally associated with insulin signaling, with insulin (INS) being the most highly connected gene within the network. Co-expressed networks correlating with neuropathological diagnostic criteria (e.g., NIA-Reagan Likelihood of AD) were associated with platelet-endothelium-leucocyte cell adhesion pathways and hypoxia-oxidative stress. Dysregulation of these functional pathways may represent incipient alterations impacting disease progression and the clinical presentation of aMCI and AD.
Frequency-dependent genetic modulation of neuronal oscillations: a combined transcriptome and resting-state functional MRI study
Authors: Liu Siyu , Zhang Cun , Meng Chun , Wang Rui , Jiang Ping , Cai Huanhuan , Zhao Wenming , Yu Yongqiang , Zhu Jiajia 
Published: January 2022

Abstract
Neuronal oscillations within certain frequency bands are assumed to associate with specific neural processes and cognitive functions. To examine this hypothesis, transcriptome-neuroimaging spatial correlation analysis was applied to resting-state functional magnetic resonance imaging data from 793 healthy individuals and gene expression data from the Allen Human Brain Atlas. We found that expression measures of 336 genes were correlated with fractional amplitude of low-frequency fluctuations (fALFF) in the slow-4 band (0.027–0.073 Hz), whereas there were no expression-fALFF correlations for the other frequency bands. Furthermore, functional enrichment analyses showed that these slow-4 fALFF-related genes were mainly enriched for ion channel, synaptic function, and neuronal system as well as many neuropsychiatric disorders. Specific expression analyses demonstrated that these genes were specifically expressed in brain tissue, in neurons, and during the late stage of cortical development. Concurrently, the fALFF-related genes were linked to multiple behavioral domains, including dementia, attention, and emotion. In addition, these genes could construct a protein–protein interaction network supported by 30 hub genes. Our findings of a frequency-dependent genetic modulation of spontaneous neuronal activity may support the concept that neuronal oscillations within different frequency bands capture distinct neurobiological processes from the perspective of underlying molecular mechanisms.
Genetically encoded voltage indicator revealed differential effects of hyperosmotic stress on yeast plasma membrane potential imposed by different stress conditions
Authors: Limapichat Walrati 
Published: January 2022

Abstract
Cells can be affected by several causes of osmotic stress by which they were forced to tolerate and adapt. An essential aspect of adaptation is ion regulation, and many insights into such complex processes can be obtained through measurement of plasma membrane potential (PMP) of cells during the stress. We recently established genetically encoded voltage indicator proteins which could be utilized to report yeast PMP change in real time. In this work, we employed this method to monitor early change in PMP of yeast Saccharomyces cerevisiae with intact cell wall, immediately following hyperosmotic up-shock due to various stress agents. The results pointed to differential effects of NaCl, sorbitol, and Polyethylene Glycol (PEG) 6000. Yeast PMP was more responsive toward PEG 6000 than NaCl and sorbitol at comparable osmotic pressure, and PEG 6000 stimulated the largest response magnitude, followed by sorbitol and NaCl, respectively. After prolonged treatments, PEG 6000 also instigated distinct cell morphology from NaCl and sorbitol. Accordingly, this study presents new evidence supporting multiple pathways underlying yeast adaptation to varying hyperosmotic conditions, enabled through the optical physiology approach. Our findings promote better understanding of yeast cellular response to hyperosmotic stress, with tenable relevance to the physiologically-related plant cells.
Marine habitat mapping to support the use of conservation and anti-trawl structures in Kep Province, Cambodia
Authors: Strong J A , Wardell C , Haïssoune A , Jones A L , Coals L 
Published: January 2022

Abstract
The marine habitats within the Kep Archipelago, Cambodia, support species of conservation importance and commercial value. Despite the area being designated a Marine Fisheries Management Area (equivalent to a Marine Protected Area locally), illegal trawling has continued to damage vulnerable habitats within the region. To augment the protection of the designated area, Conservation and Anti Trawl Structures (CATS) have been deployed locally. These structures can snare the nets of illegal trawlers and provide a hard substratum for coral colonization. A sidescan sonar survey and ground truthing campaign was used to precisely locate the 40 CATS deployed and produce maps of the important benthic habitats in the area. Due to the challenging coastal environment and minimal available infrastructure, this study used small, rechargeable or low-power (12 V), and low-cost habitat mapping equipment to map the approximate extent of several benthic habitats of conservation interest. The area and type of habitat protected by CATS has been estimated by combining the marine habitat map with the precise locations of the deployed CATS. It is hoped that this information will help inform local management decisions, such as optimizing the placement of future CATS.
Stability-Indicating New RP-UPLC Method for Simultaneous Determination of a Quaternary Mixture of Paracetamol, Pseudoephedrine, Chlorpheniramine, and Sodium Benzoate in (Cold–Flu) Syrup Dosage Form
Authors: Mohamed Mahmoud A 
Published: January 2022

Abstract

Background
A combination of paracetamol, pseudoephedrine, chlorpheniramine, and sodium benzoate in (Cold–Flu) 1,2,3 Syrup dosage form is specified for the treatment of common cold and flu symptoms.

Objective
The functional role of this study is to develop a novel, reliable, and selective stability-indicating reversed-phase ultra-performance liquid chromatography (RP-UPLC) method for simultaneous identification of a quaternary mixture of paracetamol, pseudoephedrine, chlorpheniramine, and sodium benzoate in (Cold–Flu) 1,2,3 Syrup dosage form.

Method
The specific method is accomplished using an Acquity UPLC HSS T3 C18 column (2.1 mm × 100 mm), 1.8 µm particle size with pore size 100 Å, utilizing a mixture of purified water–methanol–trifluoroacetic acid (72.5:27.5:1.5, v/v) as the mobile phase at a flow rate of 0.3 mL/min. The column void volume is 1.15 min. UPLC detection is adjusted at 205 nm using a photodiode array detector.

Results
Calibration curves are obtained in the linearity ranges: 25–500 µg/mL for paracetamol, 10–50 µg/mL for pseudoephedrine, 0.5–5 µg/mL for chlorpheniramine, and 3–30 µg/mL for sodium benzoate with a correlation coefficient &gt; 0.9992. The mean recovery of the developed method is tested and shows good recovery results between 99–101%; selectivity and forced degradation studies are investigated as per the International Council for Harmonisation Guidelines and no interference is detected due to degradation peaks.

Conclusion
The proposed stability-indicating UPLC method for simultaneous determination of the three drugs, paracetamol, pseudoephedrine, and chlorpheniramine, with a preservative sodium benzoate in (Cold–Flu) 1,2,3 Syrup dosage form is successfully accomplished, developed, and validated, and can be easily used in the analysis of drugs in pure or dosage form.

Highlights
The novelty of the current research work lies in the development of the UPLC method for simultaneous determination of a quaternary mixture of paracetamol, pseudoephedrine, chlorpheniramine, and sodium benzoate in (Cold–Flu) 1,2,3 Syrup dosage form.
Trypanosomatids Associated in the Alimentary Canal of Bagrada hilaris (Hemiptera: Pentatomidae)
Authors: Grodowitz Michael J , Gundersen-Rindal Dawn E , Elliott Brad , Evans Richard , Sparks Michael E , Reed Darcy A , Miles Godfrey P , Allen Margaret L , Perring Thomas M 
Published: January 2022

Abstract
Bagrada hilaris (Burmeister) is an invasive pest of economically important crops in the United States. During physiological investigations of B. hilaris, a flagellated protozoan was discovered in the alimentary canal of many specimens. This manuscript characterizes the morphology and molecular identification of the trypanosomatid, which appears similar to trypanosomatids identified in other stink bug species. It has been identified as a species in the Blastocrithidia genus based on morphological characteristics and molecular analyses.
The Differential Effects of Malpractice Reform: Defensive Medicine in Obstetrics
Authors: Cano-Urbina Javier , Montanera Daniel 
Published: January 2022

Abstract
Recent studies argue that different types of patients are affected differently by changes in malpractice pressure. We argue that defensive medicine causes these differential effects. Our theoretical model predicts that reduced malpractice pressure decreases health care spending among patients with good access to care, but increases spending among those with poor access. We test this theory by estimating the effects of tort reforms on birth by cesarean section. Reduced malpractice pressure through collateral source rule reform decreases C-section rates by 4.75% for mothers with timely initiation of prenatal care. On the other hand, reduced pressure through noneconomic damages caps increase C-section rates by 7.59% for mothers without timely initiation. These findings are consistent with defensive medicine. Further investigation suggests that reduced pressure improves access to care for vulnerable populations and reduces utilization among well-served consumers. These findings explain the literature’s conflicting assessments of defensive medicine, despite its practice being widespread.
Falciform ligament torsion as a rare aetiology of the acute abdomen
Authors: O’Connor Adam , Sabri Shariq , Solkar Maseera , Ramzan Amaila , Solkar Mamoon 
Published: January 2022

Abstract
The falciform ligament is a remnant of the embryonic ventral mesentery containing the obliterated umbilical vein and round ligament. It extends from the umbilicus to the superior aspect of the diaphragm. We report about a 53-year-old fit and well patient who presented with acute upper abdominal pain with tenderness to palpation. Ultrasound scan was unremarkable, but blood tests revealed raised inflammatory markers. Thus, computed tomography was performed. This demonstrated acute torsion and fat necrosis of the falciform ligament, which was the aetiology of the upper abdominal pain. Such pathology is rare with 23 previously reported cases. Conservative management is usually proposed, but on occasion, surgical intervention may be warranted in cases that do not respond to initial supportive measures. We describe this case to demonstrate a rare cause of a common presentation to the surgical service.
A novel technique for forehead and eyebrow reconstruction using a temporal hairline flap combined with a free forearm flap: a report of two cases
Authors: Hihara Masakatsu , Hikiami Rina , Mitsui Toshihito , Kakudo Natsuko , Okamoto Maki , Kuro Atsuyuki , Kusumoto Kenji 
Published: January 2022

Abstract
We herein report a novel technique for managing large forehead skin defects that include the eyebrows and upper eyelid. Case 1 was of a 55-year-old woman with an eccrine carcinoma (T2N0M0) at the right forehead, resulting in a skin defect 15 cm in diameter. Case 2 was of an 81-year-old man with a malignant peripheral nerve sheath tumor (T1a N0M0) at the left forehead, resulting in a skin defect 18 cm in diameter. A pedicled temporal hairline flap and free forearm flap were combined and placed on the defect, and good eyebrows and forehead morphology was ultimately achieved.
The legal regime for assignment of interests in Nigeria’s upstream petroleum sector: a critique of Moni Pulo vs Brass Exploration, DPR’s 2014 and 2021 Guidelines and the Petroleum Industry Act of 2021
Authors: Adaralegbe Bayo 
Published: January 2022

Abstract
Nigeria discovered oil in 1956. The Petroleum Act of 1969 that for a long time regulated the upstream petroleum sector was obsolete. This was manifest in respect of the legal regime for the transfer of upstream assets and interests. Under this regime only direct transfer of Oil Licenses or Leases were regulated. Change in control situations of Oil License or Lease holders or a merger/acquisition were not regulated. Unfortunately, Nigeria’s legislative organ, like most of the developing world where there has been political instability due to military intervention, has not been able to respond swiftly to fast-paced developments in the industry. The Petroleum Industry Act has taken some 20 years from conception to passage. In that period, the Department of Petroleum Resources (DPR) that regulated the industry found creative ways to regulate the industry in light of the failure of the National Assembly. This included issuing regulations regarding transfer of interests in the upstream sector. The judiciary was not left out and at least one case came up for determination in this regard. This article argues that case law on this point and DPR’s intervention through regulation are wrong in law and section 95 of the new Petroleum Industry Act has saved the situation.
A Labelling Semantics and Strong Admissibility for Weighted Argumentation Frameworks
Authors: Bistarelli Stefano , Taticchi Carlo 
Published: January 2022

Abstract
Argumentation Theory provides tools for both modelling and reasoning with controversial information and is a methodology that is often used as a way to give explanations to results provided using machine learning techniques. In this context, labelling-based semantics for Abstract Argumentation Frameworks (AFs) allow for establishing the acceptability of sets of arguments, dividing them into three partitions: in, out and undecidable (instead of classical Dung acceptable and not acceptable sets). This kind of semantics have been studied only for classical AFs, while the more powerful weighted and preference-based frameworks have not been studied yet. In this paper, we define a novel labelling semantics for Weighted Argumentation Frameworks (WAFs), extending and generalizing the crisp one, and we provide some insights towards a definition of strong admissibility for WAFs.
Spectral-timing of AGN ionized outflows with Athena
Authors: Juráňová A , Costantini E , Uttley P 
Published: January 2022

ABSTRACT
Spectral-timing techniques have proven valuable in studying the interplay between the X-ray corona and the accretion disc in variable active galactic nuclei (AGNs). Under certain conditions, photoionized outflows emerging from central AGN regions also play a role in the observable spectral-timing properties of the nuclear components. The variable ionizing flux causes the intervening gas to ionize or recombine, resulting in a time-dependent absorption spectrum. Understanding the spectral-timing properties of these outflows is critical not only for the determination of their role in the AGN environment but also for the correct interpretation of timing signatures of other AGN components. In this paper, we test the capabilities of the Athena X-IFU instrument in studying the spectral and spectral-timing properties of a black hole system displaying a variable outflow. We take the narrow-line Seyfert 1 IRAS 13224−3809 as a test case. Our findings show that while the non-linear response of the absorbing medium can result in complex behaviour of time lags, the resulting decrease in the coherence can be used to constrain gas density and distance to the central source. Ultimately, modelling the coherence spectra of AGN outflows may constitute a valuable tool in studying the physical properties of the outflowing gas.
Linking deep CO2 outgassing to cratonic destruction
Authors: Wang Zhao-Xue , Liu Sheng-Ao , Li Shuguang , Liu Di , Liu Jingao 
Published: January 2022

Abstract
Outgassing of carbon dioxide from the Earth's interior regulates the surface climate through deep time. Here we examine the role of cratonic destruction in mantle CO2 outgassing via collating and presenting new data for Paleozoic kimberlites, Mesozoic basaltic rocks and their mantle xenoliths from the eastern North China Craton (NCC), which underwent extensive destruction in the early Cretaceous. High Ca/Al and low Ti/Eu and δ26Mg are widely observed in lamprophyres and mantle xenoliths, which demonstrates that the cratonic lithospheric mantle (CLM) was pervasively metasomatized by recycled carbonates. Raman analysis of bubble-bearing melt inclusions shows that redox melting of the C-rich CLM produced carbonated silicate melts with high CO2 content. The enormous quantities of CO2 in these magmas, together with substantial CO2 degassing from the carbonated melt–CLM reaction and crustal heating, indicate that destruction of the eastern NCC resulted in rapid and extensive mantle CO2 emission, which partly contributed to the early Cretaceous greenhouse climate episode.
Regional aid policies after Brexit: 2nd edition
Authors: Bell David 
Published: January 2022

AbstractThis article revisits the issue of UK regional policy 5 years after the Brexit referendum. Though the mechanisms to replace EU regional investment funds are not yet in place, much has changed both in terms of understanding, and of policy relating to regional development, in the post-Brexit UK. These changes include the acknowledgement of wide regional variations in productivity and the UK government commitment to the ‘levelling up’ agenda. This has led to a number of policy initiatives, including revision of the Green Book and new funding intended to support ‘levelling up’. In addition, Brexit has necessitated the introduction of legislation to regulate the UK internal market. Its particular form may undermine the current constitutional settlement.
Impact of Hospital-Based Rehabilitation Services on Discharge to the Community by Value-Based Payment Programs After Joint Replacement Surgery
Authors: Kumar Amit , Roy Indrakshi , Warren Meghan , Shaibi Stefany D , Fabricant Maximilian , Falvey Jason R , Vashist Amit , Karmarkar Amol M 
Published: January 2022

Abstract

Objective
The purpose of this study was to examine the impact of hospital-based rehabilitation services on community discharge rates after hip and knee replacement surgery according to hospital participation in value-based care models: bundled payments for care improvement (BPCI) and comprehensive care for joint replacement (CJR). The secondary objective was to determine whether community discharge rates after hip and knee replacement surgery differed by participation in these models.

Methods
A secondary analysis of Medicare fee-for-service claims was conducted for beneficiaries 65 years of age or older who underwent hip and knee replacement surgery from 2016 to 2017. Independent variables were hospital participation in value-based programs categorized as: (1) BPCI, (2) CJR, and (3) non-BPCI/CJR; and total minutes per day of hospital-based rehabilitation services categorized into tertiles. The primary outcome variable was discharged to the community versus discharged to institutional post-acute care settings. The association between rehabilitation amount and community discharge among BPCI, CJR, and non-BPCI/CJR hospitals was adjusted for patient-level clinical and hospital characteristics.

Results
Participation in BPCI or CJR was not associated with community discharge. This analysis found a dose–response relationship between the amount of rehabilitation services and odds of community discharge. Among those who received a hip replacement, this relationship was most pronounced in the BPCI group; compared with the low rehabilitation category, the medium category had odds ratio (OR) = 1.28 (95% CI = 1.17 to 1.41), and the high category had OR = 1.90 (95% CI = 1.71 to 2.11). For those who received a knee replacement, there was a dose–response relationship in the CJR group only; compared with the low rehabilitation category, the medium category had OR = 1.21 (95% CI = 1.15 to 1.28), and the high category had OR = 1.56 (95% CI = 1.46 to 1.66).

Conclusion
Regardless of hospital participation in BPCI or CJR models, higher amounts of rehabilitation services delivered during acute hospitalization is associated with a higher likelihood of discharge to community following hip and knee replacement surgery.

Impact
In the era of value-based care, frontloading of rehabilitation care is vital for improving patient-centered health outcomes in acute phases of lower extremity joint replacement.
Equity Risk Premium Predictability from Cross-Sectoral Downturns
Authors: Faias José Afonso , Zambrano Juan Arismendi 
Published: January 2022

Abstract
We illustrate the role of left tail dependence—left tail mean (LTM)—in equity risk premium (ERP) predictability. LTM measures the average of pairwise left tail dependency among major equity sectors incorporating shocks imperceptible at the aggregate level. LTM, as well as the variance risk premium, significantly predicts the ERP in and out of sample, which is not the case with commonly used predictors. We find this predictability is the result of procyclical shocks’ reversals in a stable business cycle. This paper contributes to the ongoing debate on ERP predictability. (JEL G10, G12, G14)
Perichondrial progenitor cells promote proliferation and chondrogenesis of mature chondrocytes
Authors: Ho Chien-Liang , Huang Lynn L H , Shieh Shyh-Jou 
Published: January 2022

AbstractAutologous chondrocytes (C cells) are effective sources of cell therapy for engineering cartilage tissue to repair chondral defects, such as degenerative arthritis. The expansion of cells with C cell characteristics has become a major challenge due to inadequate donor sites and poor proliferation of mature C cells. The perichondrial progenitor cells (P cells) from the cambium layer of the perichondrium possessed significantly higher mesenchymal stem cell markers than C cells. In the transwell co-culture system, P cells increased the passaging capacity of C cells from P6 to P9, and the cell number increased 128 times. This system increased the percentage of Alcian blue-positive C cells from 40% in P6 to 62% in P9, contributing about 198 times more Alcian blue-positive C cells than the control group. C cells co-cultured with P cells also exhibited higher proliferation than C cells cultured with P cell-conditioned medium. Similar results were obtained in nude mice that were subcutaneously implanted with C cells, P cells or a mixture of the two cell types, in which the presence of both cells enhanced neocartilage formation in vivo. In aggregate, P cells enhanced the proliferation of C cells in a dose–dependent manner and prolonged the longevity of mature C cells for clinical applications.