bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of novel microsatellite markers for population differentiation and detection of natural selection in wild populations of Butter catfish, Ompok bimaculatus (Bloch, 1794)
Authors: Chowdhury Labrechai Mog , Chaturvedi Shradha , Mandal Sangeeta , Kumar Rajesh , Singh Rajeev Kumar , Lal Kuldeep Kumar , Mohindra Vindhya 
Published: October 2022

Abstract

Background: Butter catfish (Ompok bimaculatus) is a preferred species in South East Asia, with huge aquaculture potential. However, there is limited information about genetic stock composition due to insufficient markers. The goal of this study was to develop de novo microsatellite markers.
Methods and Results: For sequencing, genomic SMRT bell libraries (1.5 Kbp size) were prepared for O. bimaculatus. A total of 114 SSR containing sequences were used for primer designing and tested. Polymorphic loci were validated by genotyping 83 individuals from four distant riverine populations, viz., Brahmaputra, Bichiya, Gomti and Kaveri. A total of 30 microsatellite loci were polymorphic, of which, five were found to be associated with functional genes and eight (four positive and four negatives) loci were found to be under selection pressure. A total of 115 alleles were detected in all loci and PIC ranged from 0.539 to 0.927 and pair-wise FST values from 0.1267 to 0.26002 (p<0.001), with overall FST value of 0.17047, indicating presence of population sub-structure. Cross-species transferability of 29 loci (96.67%) was successful in congener species, O. pabda.
Conclusion: The novel SSR markers developed in this study will facilitate stock characterization of natural populations, to be used in future selection breeding programs and planning conservation strategies in these species. Identified non-neutral markers will give insights into the effect of local adaptation on genetic differentiation in the natural population of this species.
Work-Related Stress, Quality of Life, and Coping Mechanism Among Lecturers in a Tertiary Educational Institution in Anambra State, Nigeria
Authors: Chukwuemeka Uchechukwu Martha , Okonkwo Uchenna Prosper , Njoku Chibuike Jefferen , Igwe Emeka Sylvester , Oyewumi Taiwo Joseph , Ugwuanyi Daniel Chimmuanya 
Published: October 2022

Abstract

Work-related stress (WRS) is a highly prevalent and pervasive problem that can result in loss of productivity and deterioration of lecturer’s health. Lecturing work requires coping with some of the stressful situations found in any workplace. The study determined the work-related stress, coping mechanisms, and quality of life among lecturers at Nnamdi Azikiwe University (NAU).
Method:This was a cross-sectional survey involving 283 lecturers consecutively recruited from NAU. The Health and Safety Executive Work Related stress (HSE-WRS), Work-Related Quality of life (WRQL), and Brief-cope Questionnaires (BCQ) were applied to assess the participant's work-related stress, quality of work life, and coping mechanism(CM) respectively. Data were analyzed using Spearman rank and Mann-Whitney U tests at a 0.05 level of significance.
Result: Age, years of practice, and academic rank had no statistically significant relationship with work-related stress with p-values given as: p=0.96, p =0.9, and 0.21 respectively. Age and gender were significantly related to the coping mechanism with p-values: p=0.00 and p= 0.01 respectively. The quality of life of lecturers was significantly associated with age with p-values of 0.03. The years of practice have no statistically significant association with coping mechanisms.
Conclusions: there was a statistically significant relationship between age and active coping; there was a statistically significant relationship between gender and work-related stress; there was no statistically significant relationship between years of experience and QOWL; the QOWL of lecturers had a statistically significant correlation with their age, and gender affected the coping mechanism of lecturers at NAU. Also, this study revealed that male lecturers coped better with the rigorous demands of the job in contrast to female lecturers
Measuring the concentration of radioactive gas in building materials
Authors: Elaraby Entesar , Sha Doaa 
Published: October 2022

Abstract
Radon is a noble, radioactive gas produced by the radioactive decay of radium from the dissolution of uranium in soil, water, and various rocks in the environment, which leads to harm to humans and living organisms. The CR39 nuclear track detector was used to measure radioactive rates of radon in one hundred and five samples of different building materials (sand - ceramic - granite - marble -cement - gypsum - bricks) collected from the Jazan region. The average value of radon gas concentration for all samples of buildings used in the Kingdom of Saudi Arabia was 286.84 ± 33.35 Bq/Kg. The results show the highest value ​​of radiation doses in Royal Gold Granite with a value of 10.98±0.87 mSv / year. The value of the quality coefficient was a correlation between the values of radium concentration and exhalation rates of building materials. As a result, it is advised not to use granite inside homes and only place it on the front of the house.
Bioinformatics analysis of IGFBP-3 and research advances in brain tumors Running title：Protein structure and biological function
Authors: Fan Guofeng , Liu Wen , aili Yirizhati , Li Yabin , Ma Xiaohu , Liu Dong , Wang Yongxin 
Published: October 2022

Abstract
OBJECTIVE：Bioinformatics analysis of human insulin-like growth factor-binding protein-3 (IGFBP-3) molecules and genes, and review of the pathways and regulatory mechanisms of IGFBP-3 in brain tumors ( especially gliomas ) .
METHODS：Analysis of IGFBP-3 protein open reading frame（ORF）and physicochemical properties, secondary structure, tertiary structure, signal peptide and nuclear localization signal, transmembrane structure, domain, phosphorylation and glycosylation of IGFBP-3 protein using molecular biology tools Sites, proteins and biological functions that interact with IGFBP-3 proteins.
RESULTS：The molecular formula of IGFBP-3 protein is C1390H2230N418O422S21. 104 ORFs were found in the mRNA sequence of IGFBP-3. The longest ORF is ORF2, which is 519 bp in total and can encode 172 amino acids.It is an unstable hydrophilic secreted protein, the secondary structure is mainly ring structure and helical structure, containing 67 phosphorylation sites and 3 N-glycosylation sites, no transmembrane region, 3 main domains, Having nuclear localization signals exerts biological effects in the nucleus. IGFBP-3 can participate in biological functions such as apoptosis, negative regulation of cell proliferation, negative regulation of signal transduction, and regulation of insulin-like growth factor receptor signaling pathway.
CONCLUSIONS：The predicted results provide a basis for further research on the structure and biological function of IGFBP-3 gene and its encoded protein.
Accumulation of soxC-expressing cells facilitated by MMPreg is essential for blastema formation in annelids
Authors: Fujita Toshiyuki , Aoki Naoya , Mori Chihiro , Homma Koichi , Yamaguchi Shinji 
Published: October 2022

Abstract

Regeneration in many organisms involves the formation of a blastema that differentiates and organises into appropriate missing tissues. Although the mechanisms involved in blastema formation have been an intriguing topic of study in regeneration biology, the cellular and molecular basis of blastema formation remains poorly understood. Here, we demonstrate that during regeneration in E. japonensis, which can regenerate all organs from small fragments, the Sry-related high-mobility group (HMG) box (Sox) C-expressing cells accumulate in the head blastema. In addition, using RNAi silencing, we showed that MMPreg, a member of the matrix metalloprotease (MMP) family involved in extracellular matrix remodelling, is necessary for the accumulation of soxC-expressing cells in the head blastema, suggesting that MMPreg facilitated soxC-expressing cell migration to the head blastema. These findings provide insight into the cellular and molecular mechanisms underlying blastema formation.
VE-cadherin-dependent vasculogenic mimicry-like tube formation in rheumatoid arthritic synovium
Authors: Yamazaki Noritaka , Sudo Haruka , Taya Yuji , Soeno Yuuichi , Matsumoto Hideo , Okada Yasunori , Imai Kazushi 
Published: October 2022

Abstract
Increased vascularity in the synovium of patients with rheumatoid arthritis (RA) is thought to result from angiogenesis. However, other possibilities to develop vasculatures by mechanism(s) like vasculogenic mimicry remain elusive. In this study, expression of vascular endothelial (VE)-cadherin was immunohistochemically detected in both microvessel lining cells and fibroblastic cells in RA synovium. VE-cadherin-positive lining cells surrounded by periodic acid-Schiff staining-positive substance were occasionally negative for endothelial cell markers CD31/CD34 but positive for osteoblast (OB)-cadherin that is specific to fibroblastic cells. RA synovial fibroblast-like cells (RSFLs) in culture expressed VE-cadherin and OB-cadherin, but not CD31/CD34. Tube formation was evaluated by real-time adhesion and tube formation assays. RSFLs specifically bound to VE-cadherin-chimera-coated plates. They developed tube-like structures solely, which were hindered by anti-VE-cadherin antibody or VE-cadherin-chimera, and with endothelial cells. VE-cadherin was up-regulated by vascular endothelial growth factor-A165 (VEGF165) treatment, and shed from cell surface by treatment with tumor necrosis factor-a. RSFLs expressed VEGF and VEGF receptors (VEGFRs), and VEGF165 induced up-regulation of VE-cadherin was suppressed by inhibiting VEGFR2 activity. These data demonstrate that fibroblastic cells in RA synovium express VE-cadherin, and suggest that the fibroblastic cells are involved in formation of vasculogenic mimicry-like tubes in RA synovium through the VEGF/VEGFR2/VE-cadherin pathway.
The Temperature Dependence of Hot Prograde Flows in Solar Active Regions
Authors: Fitzpatrick Jimmy C. , Hudson Hugh 
Published: October 2022

Abstract
Using simultaneous observations from the EUV Variability Experiment (EVE) and imaging from the Atmospheric Imaging Assembly (AIA), we characterise the temperature dependence of apparent hot flows in solar active regions. The EVE instrument performs Sun-as-a-star spectroscopy and is composed of two spectrographs, MEGS-A and MEGS-B. It is known that EVE can measure wavelength shifts, and thus can observe relative Doppler velocities in solar atmospheric plasmas over an extended temperature range. However, MEGS-A is a↵ected by a known astigmatism e↵ect (Chamberlin, 2016); inho-mogeneities in EUV brightness on the solar surface result in purely instrumental wavelength errors. We validate our methods by independently quantifying this e↵ect and comparing to Chamberlin’s results, and explore the wavelength dependence as an extension of his formula as derived for He ii 304Å304Å. MEGS-B is una↵ected by this instrumental e↵ect in any case, and this has allowed us to find evidence of hot prograde flows in active regions. Using our image-based models for astigmatism and flows, we independently confirm the MEGS-B results (Hud-son et al., 2022). We now extend our knowledge of the temperature dependence of these flows via the additional Fe emission lines available in MEGS-A. We find a monotonic increase of apparent flow speed with temperature up through lines of Fe xvi, nominally formed at about 6.4 MK.
Faster Algorithm for Finding Maximum 1-Restricted Simple 2-Matchings
Authors: Artamonov Stepan , Babenko Maxim 
Published: October 2022

Abstract
We revisit the problem of finding a 1-restricted simple 2-matching of maximum cardinality. Recall that, given an undirected graph G = (V, E), a simple 2-matching is a subset M ⊆ E of edges such that each node in V is incident to at most two edges in M. Clearly, each such M decomposes into a node-disjoint collection of paths and circuits. M is called 1-restricted if it contains no isolated edges (i.e. paths of length one). A combinatorial polynomial algorithm for finding such M of maximum cardinality and also a min-max relation were devised by Hartvigsen. It was shown that finding such M amounts to computing a (not necessarily 1-restricted) simple 2-matching M0 of maximum cardinality and subsequently altering it into M of the same cardinality so as to minimize the number of isolated edges. While the first phase (which computes M0) runs in O(E√V ) time, the second one (which turns M0 into M) requires O(V E) time. In this paper we apply the general blocking augmentation approach (initially introduced, e.g., for bipartite matchings by Hopcroft and Karp, and also by Dinic) and present a novel algorithm that reduces the time needed for the second phase to O(E√V ) thus completely closing the gap between 1-restricted and unrestricted cases.
Perception of a need to change weight in individuals living with and beyond breast, prostate and colorectal cancer: a cross-sectional survey
Authors: Heuchan Gabriella N , Lally Phillippa J , Beeken Rebecca J , Fisher Abigail , Conway Rana E 
Published: October 2022

Abstract
Purpose
People living with and beyond cancer (LWBC) are advised to achieve a body mass index (BMI) within the healthy range (≥18.5 and <25). Not perceiving a need for weight change may be a barrier to achieving a healthy weight. This study aimed to explore factors associated with perceived need for weight change among people LWBC.
Methods
Adults diagnosed with breast, prostate, or colorectal cancer were recruited through National Health Service sites in Essex and London. Participants (N=5,835) completed the ‘Health and Lifestyle After Cancer’ survey, which included a question on perceived need to change weight. Associations between perceived need for weight change and BMI, and perceived need for weight change and health and demographic variables, were analysed using chi-square tests and logistic regression, respectively.
Results
The proportion of participants perceiving a need to lose weight differed according to BMI category: healthy weight (23%), overweight (64%), obese (85%) (P<0.001). Having overweight or obesity but not perceiving a need to lose weight was associated with being older, male, non-white, not married or cohabiting, and having cancer that had spread, no formal qualifications, and no comorbidities.
Conclusions
Perceived need to lose weight is prevalent among people LWBC with obesity and overweight. This group may be interested in weight management support. Demographic and health factors were associated with having obesity or overweight but not perceiving a need to lose weight.
Implications
Weight loss interventions for people LWBC are needed. A subset of people LWBC with overweight and obesity may need additional information or motivators to engage with weight management.
A systematic description of continent ileostomy revision techniques
Authors: Ecker Nils Karl Josef , Tönsmann Mathias , Ecker Karl-Wilhelm 
Published: October 2022

Abstract
Based on the anatomic site and severity of the respective complication, a systematically classified approach to the appropriate techniques for continent ileostomy revision surgery is presented. By analyzing 133 revision procedures performed over 30 years, four classes of complications are defined: class A refers to the nipple valve, class B to the pouch, class C to the stoma, and class D to the afferent loop. The severity of the complication or complexity of the revision procedure is surmised by a subdivision from a to d. The resulting surgical variants are shown in schematic illustrations with accompanying descriptions of technical details, the respective fields of application, and the special indications. Trial registration numbers: None
Relation Correlations-Aware Graph Convolutional Network With Text-Enhanced for Knowledge Graph Embedding
Authors: Yu Hong , Tang Jinxuan , Peng Zhihan , Liu Dun 
Published: October 2022

Abstract

Long-tail distribution is a diffiffifficult challenge for knowledge graph embedding.We expect to solve the problem by complementing the information of a few shot relations through the neighbor aggregation mechanism of GCN. However, the GCN method and its derivations ignore semantic information and are unable to learn the representation of edges. To address this problem, we propose RCGCN-TE, Relation Correlations-aware Graph Convolutional Network with Text-Enhanced for knowledge graph embedding. As far as we know, this is the fifirst effffort to enable GCN to learn the representation of relations directly. First, the pre-trained language model is used to extract semantic information from description text. Then, the relation correlation graph is constructed by defifining the relation relevance function based on the co-occurrence pattern and semantic similarity of relations. Finally, two GCNS are designed to learn entities and relations respectively, with the loss function allowing them to learn jointly. The experimental results reveal that the proposed model outperforms the baselines on the tasks such as triple classifification and link prediction. For example, Hits@10, Hits@3, and Hits@1 improved by 8.23%, 37.49%, and 46.94%, respectively, on the entity prediction task.
Caffeine Improves Elevated IOP by modulating Oxido-inflammatory responses in Rat Models of Glaucoma
Authors: Adekeye Adeshina , Fafure Adedamola , Mellah Faith Seember , Zubairu Mahmud Kamaru , Enye Linus , Oni James Olukayode 
Published: October 2022

Abstract

Glaucoma, a neurodegenerative disease caused by continuous damage to the retina and optic nerve, is the leading cause of irreversible blindness globally. Caffeine, a central nervous system stimulant, is widely consumed for its psychoactive effects. This study aimed to determine caffeine's anti-oxidative and anti-inflammatory role on elevated intraocular pressure (IOP) following hyaluronic acid and hypertonic saline injections. Thirty (30) adult Long-Evans rats were distributed randomly into six (n=6) groups. The Control group received 50 µL of dH20. H.A. received 25 µL of hyaluronic acid into the corneosclera junction. H.S., 50 µL of hypertonic saline into the episcleral vein. PHA and PHS were treated with an intraperitoneal injection of 20mg/kg caffeine. Group CAF received an intraperitoneal injection of 20 mg/kg of caffeine. IOP measurement was taken, and markers of oxidative stress, malondialdehyde (MDA), superoxide dismutase (SOD) were assayed. The inflammasome immunoreactivity was evaluated. We observed severe inflammasome activation in the H.S. model of elevated IOP. H.A. and H.S. injections induced antioxidant imbalance by increased and decreased levels of MDA and SOD, respectively. In addition, an increase in the IOP and retina damage was observed following H.A. and H.S. injection, while caffeine demonstrated an ameliorative role in reviving the RGC damage. Caffeine demonstrated an ameliorative role in reducing the intraocular pressure and rejuvenating effect on the RGC, which was more evident in the hypertonic saline model of elevated IOP.
Criteria for the use of conventional clip and over-the-scope clip for closure of mucosal defects after duodenal endoscopic submucosal dissection
Authors: Jinushi Ryuhei , Tashima Tomoaki , Sato Ryo , Komori Kengo , Shin Takahiro , Terada Rie , Shiomi Rie , Muramatsu Takahiro , Ishikawa Tsubasa , Fujita Akashi , Tanisaka Yuki , Mashimo Yumi , Mizuide Masafumi , Kawasaki Tomonori , Ryozawa Shomei 
Published: October 2022

Abstract
Purpose

Over-the-scope clip (OTSC) is used for treatment of gastrointestinal perforation, postoperative anastomotic leakage, and for mucosal defect closure after duodenal endoscopic submucosal dissection (ESD). However, OTSCs are expensive and associated with fatal complications; therefore, proper OTSC usage is necessary. There are no clear criteria for OTSC use for mucosal defect closure after duodenal ESD. Therefore, we examined the closure outcomes achieved using OTSCs or conventional clips for patients that underwent duodenal ESD. We also analyzed the resected specimen area and preoperative estimated size of tumors treated with each method to determine the criteria for using either OTSC or conventional clip.

Methods

Endoscopic resection was performed for 133 superficial duodenal epithelial tumors at our institution from April 2017 to February 2022. Complete closure of mucosal defects after duodenal ESD was attempted for 82 tumors; these were divided into the OTSC group and the control group (for which conventional clips were used). Closure outcomes were analyzed.

Results

The overall rate of complete mucosal defect closure in the OTSC and control groups was 98.8% (95% confidence interval = 93.39–99.97%). There were significant differences in the median estimated tumor size (20 mm vs 15 mm; p < 0.001) and median resected specimen area (339.1 mm2 vs 169.6 mm2; p < 0.001) between the groups.

Conclusion

If the estimated preoperative tumor size is ≤ 18 mm, complete closure of mucosal defects after duodenal ESD can be achieved with only conventional clips. Based on this study, we suggest that OTSC is not necessary for small lesions.
A Novel Metaheuristic Method: Gravitational-based Global Optimization
Authors: ERTUĞRUL Ömer Faruk , ALDEMİR Erdoğan , ÖZTEKİN Abdulkerim , ACAR Emrullah 
Published: October 2022

Abstract
Metaheuristic optimization methods provide a satisfactory solution for complex engineering problems. In this study, a novel metaheuristic searching approach was proposed to tackle engineering problems. The relative slope-based gravitational searching algorithm, namely XAR, provides a novel searching strategy, which is built on the law of gravity and interaction of the inertia mass. Making to move the searching agents using the slope-based gravity is the original contribution to this work. Searching agents of the method, i.e., balls, evolutionarily move regarding the relative slope of the consecutive balls. The algorithm determines a set of resolutions by the numbers of the balls aggregated. In other words, the method converges to the area in search space, where the greatest number of agents is located. The algorithm has been tested on a wide range of benchmark function sets and a complex real-world engineering problem. The implementation results confirm a notable achievement for a diverse set of cost functions. The results demonstrate that the proposed algorithm achieves a notable achievement of global optimum compared to the state-of-the-art methods. Furthermore, a satisfactory rate of convergence for all functions was found. In other word, the method converges to the optimal position (minimum cost) in search space, where the greatest number of agents is located.