bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the Oral and Dental Health Awareness Levels of Pregnant Individuals from a Sociocultural and Socioeconomİc Point of View: A Pilot Study from Turkey
Authors: YILDIRIM Ozgun , TURKMENOGLU Kadir , MOLLAOGLU Nur 
Published: October 2022

Abstract
Background
Hormonal changes occur during pregnancy and their effects on oral and dental health have been investigated for a long time. In this study, it was aimed to investigate the sociocultural and socioeconomic status of pregnant patients who applied to the department of oral and maxillofacial surgery for tooth extraction as well as their level of awareness about oral and dental health.
Methods
A questionnaire including total of 16 questions about their awareness, practices and attitudes towards oral and dental health was filled by 193 pregnant volunteers, and the results were evaluated statistically.
Results
When the attitudes of pregnant women towards oral and dental health were examined according to their monthly income, it was found that there was no statistically significant difference (p > 0.05). However, it was determined that the attitudes of pregnant women with a middle income towards oral and dental health were better. In addition, it was determined that there was a statistically significant difference in the level of consciousness between those who had a dental examination during pregnancy and those who did not (p < 0.05).
Conclusion
Not all the pregnant women are always aware that they may have problems by their oral and dental health during their pregnancy and moreover, they are not always well informed by the health personnel. It seems there is a lack of dental health controls in Turkey for pregnants. This pilot study indicates that oral health screening needs to be a routine procedure of antenatal clinics in Turkey in order to inform pregnants in detail regardless of their sociocultural and socioeconomic status to reach better oral health and consequently better pregnancy outcomes.
Early flowering of the transgenic rice with AtNF-YB1
Authors: Peng Meifang , Gan Feng , Pan Chunmei , Lin Xiaomin , Lin Feng , Ren Yuanhang , Na Shungui , Zhu Xinhai , Tang Wenwen , Wu Zhixue , Fan Xiaoli , Chen Kegui 
Published: October 2022

Abstract
Grain yield and growth period are two critical agronomic traits for a rice cultivar to be used in the field. The growth period is determined by flowering time, which also greatly affects grain yield. An Arabidopsis gene, AtNF-YB1, was introduced into rice, including Kasalath and two hybrids, Jinfeng X Chenghui 727 and Jinfeng XChuanhui 907. All the transgenic rice showed early flowering in both natural long-day (NLD) and natural short-day (NSD) conditions because of suppression of Ghd7 transcription resulting in early activating the flowering transition. The of pure line of Kasalath with the gene showed also shorter plant height and less grain yield with a decrease in spike length and grain number but more productive panicle. But, for the hybrids, a little or no reduction of spike length, grain number, and more productive panicles were observed so that grain yields were kept or even increased under NLD conditions. RNA-Seq further demonstrated that three pathways related to plant photosynthesis were markedly up-regulated in the transgenic Jinfeng B and hybrid Jinfeng X Chuanhui 907. Measurements of photosynthetic parameters indicated that photosynthetic rates were up-regulated with an increase of chlorophyll contents in the transgenic Jinfeng B and hybrid Jinfeng XChuanghui 907. The results suggest that AtNF-YB1could be useful for creating rice variety with early ripening, particularly for hybrid rice.
Adaptive Resistance to Lorlatinib via EGFR Signaling in ALK-rearranged Lung Cancer
Authors: Yamada Tadaaki , Katayama Yuki , Tanimura Keiko , Tokuda Shinsaku , Morimoto Kenji , Hirai Soichi , Matsui Yohei , Nakamura Ryota , Ishida Masaki , Kawachi Hayato , Yoneda Kazue , Hosoya Kazutaka , Tsuji Takahiro , Ozasa Hiroaki , Yoshimura Akihiro , Iwasaku Masahiro , Kim Young , Horinaka Mano , Sakai Toshiyuki , Takayama Koichi 
Published: October 2022

Abstract
Anaplastic lymphoma kinase (ALK)-tyrosine kinase inhibitors rarely elicit complete responses in patients with advanced ALK-rearranged non-small cell lung cancer (NSCLC), as a small population of tumor cells survives due to adaptive resistance. Therefore, we focused on the mechanisms underlying adaptive resistance to lorlatinib and therapeutic strategies required to overcome them. We found that epidermal growth factor receptor (EGFR) signaling was involved in the adaptive resistance to lorlatinib in ALK-rearranged NSCLC, activation of which was induced by heparin-binding EGF-like growth factor production via c-Jun activation. EGFR inhibition halted ALK-rearranged lung cancer cell proliferation by enhancing ALK inhibition-induced apoptosis via suppression of Bcl-xL. Xenograft models showed that the combination of EGFR inhibitor and lorlatinib considerably suppressed tumor regrowth following cessation of these treatments. This study provides new insights regarding tumor evolution due to EGFR signaling after lorlatinib treatment and the development of combined therapeutic strategies for ALK-rearranged lung cancer.
Toxin expression during Staphylococcus aureus infection imprints host immunity to inhibit vaccine efficacy
Authors: Teymournejad Omid , Li Zhaotao , Beesetty Pavani , Yang Ching , Montgomery Christopher 
Published: October 2022

Abstract
Staphylococcus aureus infections are a major public health issue and a vaccine is urgently needed. Despite considerable promise in preclinical models, all vaccines tested thus far have failed to protect humans against S. aureus. Unlike laboratory mice, humans are exposed to S. aureus throughout life. In the current study, we hypothesized that prior exposure to S. aureus “imprints” the immune response to inhibit vaccine-mediated protection. We established a mouse model in which S. aureus skin and soft tissue infection (SSTI) is followed by vaccination and secondary SSTI. Unlike naïve mice, S. aureus-sensitized mice were incompletely protected against secondary SSTI by vaccination with the inactivated α-hemolysin (Hla) mutant HlaH35L. Inhibition of protection was specific for the HlaH35L vaccine and required hla expression during primary SSTI. Surprisingly, inhibition occurred at the level of vaccine-elicited effector T cells; hla expression during primary infection limited the expansion of T cells and dendritic cells and impaired vaccine-specific T cell responses. Importantly, the T cell-stimulating adjuvant CAF01 rescued inhibition and restored vaccine-mediated protection. Together, these findings identify a potential mechanism for the failure of translation of promising S. aureus vaccines from mouse models to clinical practice and suggest a path forward to prevent these devastating infections.
Shorter Planning Depth and Higher Response Noise During Sequential Decision-Making in Old Age
Authors: Steffen Johannes , Markovic Dimitrije , Glöckner Franka , Neukam Philipp T. , Kiebel Stefan J. , Li Shu-Chen , Smolka Michael N. 
Published: October 2022

Abstract
Forward planning is crucial to maximize outcome in complex sequential decision-making scenarios. In this cross-sectional study, we were particularly interested in age-related differences of forward planning. We presumed that especially older individuals would show a shorter planning depth to keep the costs of modelbased decision-making within limits. To test this hypothesis, we developed a sequential decision-making task to assess forward planning in younger (age < 40 years; n = 25) and older (age > 60 years; n = 27) adults. By using reinforcement learning modelling, we inferred planning depths from participants' choices. Our results showed significantly shorter planning depths and higher response noise for older adults. Age differences in planning depth were only partially explained by wellknown cognitive covariates such as working memory and processing speed. Consistent with previous findings, this indicates agerelated shifts away from modelbased behaviour in older adults. In addition to a shorter planning depth, our findings suggest that older adults also apply a variety of heuristical low-cost strategies.
The efficacy of postoperative antiviral therapy in elderly HCC patients: a retrospective multicenter propensity score-matched study
Authors: Zhang Jinyu , Wu Qionglan , Zeng Jianxing , Zeng Jinhua , Liu Jingfeng 
Published: October 2022

AbstractPurpose We investigated the effects of postoperative adjuvant antiviral therapy (AVT) on elderly patients following curative treatment for hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Methods A total of 300 elderly patients with HBV-related HCC were recruited, which comprised 123 non-AVT groups and 177 AVT groups. Propensity score matching (PSM) was developed to reduce any bias in patient selection. Independent risk factors were identified by Cox regression analysis. Results After PSM, the 1-, 3-, and 5-year OS rates in the AVT group and non-AVT group were 97.5%, 76.0%, 55.0%, and 83.5%, 60.1%, 40.9%, (p = 0.003), respectively. The 1-, 3-, and 5-year RFS rates in the AVT group and non-AVT group were 76.6%, 50.2%, 30.4%, and 63.2%, 42.4%, 26.6% (p = 0.120), respectively. Multivariate Cox analysis revealed that postoperative adjuvant AVT was the independent protective factor associated with mortality (HR = 0.46, 95%CI = 0.31–0.68, p < 0.001). Conclusions Among patients who underwent curative hepatectomy for HBV-related elder HCC. Postoperative adjuvant AVT can improve long-term survival and was the independent protective factor associated with survival after PSM.
Patent Ductus Arteriosus (PDA) and Response to Late Surfactant Treatment in Premature Infants
Running Title: Ductus Arteriosus and Late Surfactant Treatment
Authors: Beauchene Madeline , Cunningham Alison , Stanford Amy , Bischoff Adrianne , Dagle John , Rios Danielle , Klein Jonathan , Giesinger Regan , McNamara Patrick 
Published: October 2022

Abstract
Objective
To determine clinical/echocardiography (ECHO) phenotype of response to late surfactant, according to patent ductus arteriosus (PDA) status.
Study Design
Infants ≤26+6 weeks gestational age (GA), who received ≥1 surfactant dose after postnatal day 6 with known PDA status, were enrolled. Response to surfactant, based on change in respiratory severity score, and relationship with PDA status were evaluated via univariate analysis.
Results
In total, 35 patients were enrolled (total of 71 doses of surfactant), whose mean weight and GA at birth were 595g(508, 696) and 23.3(22.7, 25) weeks. Positive response to late surfactant was independently associated with absence of PDA [OR 26(2, 334), p=0.01] whereas presence of PDA was associated with negative response [OR 12(1.1, 126), p=0.04].
Conclusions
In neonates ≤26+6 weeks gestation response to late surfactant is influenced by PDA status. Future trials should consider PDA status when refining patient selection for late surfactant treatment.
Microencapsulation of Lactobacillus plantarum in the Alginate/Chitosan improves immunity, disease resistance, and growth of Nile tilapia (Oreochromis niloticus)
Authors: Bahrami Zahra , Roomiani Laleh , Javadzadeh Narges , Sary Aboalfazl Askary , Javaheri Mehran 
Published: October 2022

Abstract
This study evaluated the effects of microencapsulation of L. plantarum (as a probiotic) ‎with chitosan/alginate biopolymers ‎(MLCA) on innate immune response, disease resistance, and growth performance of Nile tilapia (Oreochromis niloticus). Four hundred and eighty fish were randomly distributed in glass tanks (150 L) and fed with diets including Diet 1: Control; ‎‎ Diet 2: 10 g kg-1 microcapsules‎; Diet 3: 108 CFU g-1 L. plantarum ; and Diet 4: ‎10 g kg-1 MLCA for 60 days. The hematology and biochemical indices, lysozyme activity, alternative complement activities, respiratory burst, serum bactericidal activity as well as growth performance parameters (specific growth rate, feed conversion ratio) were assayed. White blood cells, plasma protein and globulin concentration, serum lysozyme, and respiratory burst activities of fish were significantly increased in the MLCA diet. A challenge test against Streptococcus agalactiae , at the end of the experiment, showed the highest survival rate of the fish fed with MLCA. Moreover, the fish fed with MLCA showed a significant improvement in SGR and FCR and had the highest growth performance. These results suggest longer stability of probiotics in the microcapsules, and their immunomodulatory effect can be considered a promising immunostimulant and growth enhancer in the Nile tilapia diet.
Screening of breast cancer diagnostic and prognostic biomarkers using bioinformatics analysis
Authors: Xu Yuehong , Niu Changchun 
Published: October 2022

Abstract
Background Breast cancer is one of the most common malignant tumors in women, and its incidence is increasing year by year and tends to be younger, which seriously threatens women's life and health. Therefore, the search for more sensitive and specific diagnostic and prognostic biomarkers for breast cancer is an urgent need. Object Screening potential diagnostic and prognostic biomarkers of breast cancer using bioinformatics analysis.
Method GEO2R was used to analyze the gene differential expression of the breast cancer chips screened in the comprehensive gene expression database, and the DAVID online analysis website was used to conduct GO enrichment analysis and KEGG pathway enrichment analysis of the differential genes and visualized them by R language. Finally, the five genes with the most significant differences were screened for further analysis, and the survival analysis of the five most significant genes was carried out through the GEPIA online analysis website, and the expression levels of these five differential genes were verified in the TCGA database and GTEx database.
Result Through differential analysis of cancer samples from breast cancer patients and normal breast samples, a total of 965 differential genes were obtained, 833 were down-regulated and 132 were up-regulated. Differentially expressed genes were enriched for different GO subsets such as angiogenesis, plasma membrane, and integrin binding, PPAR signaling pathways, regulation of lipolysis in adipocytes, and glycerollipid metabolism. The five genes with the most significant differences were CA4, PLIN4, GPD1, TUSC5, and S100B, and the expression levels of these five genes in breast cancer tissues were lower than those in normal breast tissues. GEPIA online analysis of the five most significantly differentially expressed genes, we found that the gene S100B has a significant relationship with the prognosis of patients. The higher the expression of the S100B gene, the better the prognosis of the patient. However, the expression levels of CA4, PLIN4, GPD1, and TUSC5 genes were not significantly associated with the prognosis of patients. The expression levels of these five genes in the TCGA database and GTEx database were down-regulated in breast cancer, and there was statistical significance.
Conclusion The five most significantly differentially expressed genes, CA4, PLIN4, GPD1, TUSC5, and S100B, are expected to be potential diagnostic biomarkers for breast cancer patients. The gene S100B has prognostic value for breast cancer patients. The higher the expression of the S100B gene, the better the prognosis of patients.
How Do Cancer Patients Refuse Treatment? A Grounded Theory Study
Authors: Khankeh Hamid Reza , Vojdani Reza , Saber Mahboobeh , Imanieh Mohamadhadi 
Published: October 2022

Abstract
Background
All cancer patients, except for a small fraction, seek treatment after becoming aware of the disease. That small fraction do not seek any treatment due to various reasons, and this phenomenon is unknown to us. Therefore, the present study aimed to discover the reasons for treatment refusal in cancer patients.
Methods
This qualitative grounded theory study was conducted on 22 participants including patients, caregivers, physicians, and nurses. Purposive theoretical sampling was employed. Data were collected through in-depth interviews. All interviews were gradually transcribed and analyzed. Data analysis was carried out through the three-step method of open, axial, and selective coding and was continued until theoretical saturation. Straussian Grounded Theory was used for data analysis.
Results
A total of 4 themes and 20 sub-themes were extracted in this study. The core variable extracted from the interviews was "resilience" Other related themes included encounter with cancer, fighting cancer, and coping with cancer. The findings showed that in the context of fighting cancer, patients lost their tolerance through various processes and refused treatment.
Conclusion
Cancer patients abandon the treatment in silence, oncologists and even family members being unaware of the matter. In other words, refusal of treatment is like an iceberg and the majority of the patients who have abandoned treatment are unknown to the health system. The model obtained in this study can increase the knowledge of the process that leads patients to lose their tolerance against cancer and abandon treatment, which can increase the possibility of recognizing and predicting treatment refusal for oncologists.
The characters of root-derived fungi from Gentiana scabra Bunge and the relations with their habitats
Authors: Hu Tianshuai , Wang Fuqi , Wang Dongmei , Guo Ruixue , Zhai Ying , Wang Huan , Cheng Cheng , Ding Wenjing , Ren Shumeng , Pan Yingni , Liu Xiaoqiu 
Published: October 2022

Abstract
Aims
Gentiana scabra Bunge (GTSB) root-derived fungi including endophytic fungi (EF) and rhizospheric fungi (RF) are key factors which influence the quality and fitness of this important herb.
Methods
We determined the population, diversity, and the dominant type of EF and RF in GTSB obtained from Liaoning, Yunnan and Jilin province, China by using ITS rRNA cloning and sequencing methods. Meanwhile, we investigated the potential influence of different habitats on fungi communities, and analyzed the correlation between fungi communities, the main active metabolites of Gentian polysaccharide and gentiopicroside in GTSB and soil physicochemical properities.
Results
5,419 fungal isolates, belonging to 1 domain, 1 kingdom, 8 phyla, 32 classes, 104 orders, 280 families, 630 genera, 1,238 species were obtained. The population and diversity of EF and RF varied in both habitats and months, showing a significant difference. Among them, the predominant genera of EF were Lecidella and unclassified_p_Ascomycota, while the predominant genera of RF were complicated. Compared to EF, the diversity of rhizosphere fungi is more affected by soil physicochemical properties, including pH, water content, alkali-hydrolyzable nitrogen, available phosphorus, etc. Additionally, we found that the accumulation of gentiopicroside was positively correlated with EF of Epicoccum and RF of Mortierella, Solicoccozyma, Talaromyces and Trichoderma. The accumulation of Gentian polysaccharide was negatively correlated with EF of Lenzites, Mucor, Myrothecium and Saccharomycopsis and rhizosphere fungi, such as Botrytis, Cadophora, Cladophora, Didymela, Fusarium, etc.
Conclusions
This work will guide the development strategies to cultivate high-quality Gentiana scabra Bunge in the future and provide new ideas for the study of other medicinal plants.
1H, 15N and 13C resonance assignments of a minimal CPSF73-CPSF100 C-terminal heterodimer
Authors: Thore Stéphane , Fribourg Sébastien , Mackereth Cameron D. 
Published: October 2022

Abstract
The initial pre-mRNA transcript in eukaryotes is processed by a large multi-protein complex in order to correctly cleave the 3’ end, and to subsequently add the polyadenosine tail. This cleavage and polyadenylation specificity factor (CPSF) is composed of separate subunits, with structural information available for both isolated subunits and also larger assembled complexes. Nevertheless, certain key components of CPSF still lack high-resolution atomic data. One such region is the heterodimer formed between the first and second C-terminal domains of the endonuclease CPSF73, with those from the catalytically inactive CPSF100. Here we report the backbone and sidechain resonance assignments of a minimal C-terminal heterodimer of CPSF73–CPSF100 derived from the parasite Encephalitozoon cuniculi. The assignment process used several amino-acid specific labeling strategies, and the chemical shift values allow for secondary structure prediction.
Effective role of dietary curcumin nanoparticles and Spirulina platensis supplementation on growth, digestive enzymes, hematological, serum biochemical parameters, antioxidant status, immune responses, and histological examination in Nile tilapia fingerlings
Authors: Eissa El-Sayed Hemdan , Elbahnaswy Samia , Elshopakey Gehad E. , Alamoudi Muna Omer , Aljarari Rabab Mohamed , Khattab Marwa S. 
Published: October 2022

Abstract

The positive effects of feeding nano-curcumin and Spirulina platensis onO. niloticus's growth performance, hepatoprotective ability, antioxidant response, immunity, digestibility, and organ histopathology were predicted by this study. Nile tilapia fingerlings (n = 180 fish; 10 ± 0.5 g) were divided into three groups (three replicates/group) and fed diets containing zero percent (control), 30 mg kg-1 curcumin nanoparticles (Cu-NPs group), and 5 g kg-1Spirulina platensis(SP group). Growth performance, haematological, antioxidant, biochemical, and immunological markers plus histological changes were evaluated after the feeding study lasted 56 days. Our results revealed that Cu-NPs and SP have favorable effects on carcass composition, growth performance, hematological, and biochemical parameters. Moreover, Cu-NPs and SP supplementation significantly elevated serum Ig M level (p < 0.01,p < 0.05), lysozyme (p < 0.001), amylase (p < 0.05,p < 0.01), lipase (p < 0.05) and protease (p < 0.01) activities unlike the control group. Additionally, significant elevation of SOD (p < 0.001) and GPx (p < 0.01,p < 0.05) activities with diminished MDA (p < 0.001,p < 0.01) production was noticed in SP and Cu-NPs groups compared to control one. Overall, diets supplemented with Cu-NPs and SP effectively improved the growth performance, hepatic function, immune response, and antioxidant impacts in Nile tilapia fingerlings.