bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cirrhosis and Pregnancy: A Single Centre Experience
Authors: Imamoglu Metehan , Ekici Huseyin , Okmen Firat , Ergenoglu Mete 
Published: February 2022

Abstract
PurposeCirrhosis is a diffuse pathology characterized by fibrosis of the liver and is the last stage of chronic liver diseases. It is a serious medical condition which seriously impacts reproduction and reproductive life span. MethodsRetrospective chart review of the fetal and maternal results of 20 pregnant women with liver cirrhosis who had undergone antenatal follow-up and delivery at a tertiary center in a 12-year period was performed. ResultsChronic hepatitis B was found to be the leading cause of liver cirrhosis in the study group, with a rate of 25 % (n: 5/20). The average MELD score was calculated as 8.8 ± 3.5. Only three patients developed hepatic decompensation during pregnancy. Fetal demise was observed in 10% of the cases (n: 2/20, MELD scores 8 and 17).ConclusionEven though pregnancy is rarely observed in women with liver cirrhosis, many patients with are able to achieve favorable maternal and fetal results without developing hepatic decompensation with appropriate management and close follow-up. The Model for End-Stage Liver Disease (MELD) score is a clinical tool utilized to estimate the severity and survival for chronic liver disease and was previously found to be associated with unfavorable outcomes in pregnant patients. Our study confirms this finding with the current experience from a tertiary care center. In addition, with the effects of cirrhosis on pregnancy being a concern, the impact of pregnancy on the course of the disease should also be addressed.
The changes of serum fibrinogen-like protein 1 levels associated with improved liver function and uric acid after laparoscopic sleeve gastrectomy
Authors: Qu Shen , Dilimulati Diliqingna , Du Lei , Huang Xiu , Cai Meili , Zhang Yuqin , Zhou Donglei , Zhu Jiangfan , Su Lili , Zhang Manna 
Published: February 2022

Abstract
Background: Fibrinogen-like protein (FGL)-1 is a novel hepatokine that plays an important role in the development of hepatic steatosis and insulin resistance. The purpose of this study is to analyze serum FGL-1 levels in patients with obesity before and after laparoscopic sleeve gastrectomy (LSG). Methods: Ninety-two individuals (32 people with normal weight and 60 patients with obesity) were included in this study. All patients with obesity finished follow-up visits at 6 months after LSG. Clinical data, anthropometric parameters and biochemical variables were collected. Serum FGL-1 levels were assessed by enzyme-linked immunosorbent assay (ELISA). Results: FGL-1 levels in patients with obesity (44.66 ± 20.03 ng/mL) were higher than individuals with normal weight (20.73 ± 9.73 ng/mL, P < 0.001). At baseline, FGL-1 levels were positively correlated with body mass index (BMI), blood pressure, hepatic enzyme levels, glucose metabolism, lipid metabolism and uric acid (UA). After LSG, FGL-1 levels were significantly decreased (27.53 ± 11.45 ng/mL, P < 0.001). Besides, BMI, hepatic enzyme levels, glucose metabolism, lipid metabolism and UA were significantly improved. After adjusting possible confounders, changes in FGL-1 levels at 6 months after surgery were positively correlated with changes in ALT, AST and UA levels.Conclusions: FGL-1 levels were closely correlated with various metabolic factors and decrease following LSG in patients with obesity. Furthermore, FGL-1 levels were more closely related to liver function and uric acid metabolism, but not body weight and glucolipid metabolism.Trial registration: This study protocol was approved by the Ethical Committee of Shanghai Tenth People’s Hospital. The Clinical Registration Number is ChiCTR-OCS-12002381. Date: 23 July 2012.
Optimal Synthesis of Algae@Cu Hybrid Nanoflower And Their Antioxidant And Catalytic Activities
Authors: Koca Fatih Doğan , Muhy Haydar Matz , Halici Mehmet Gökhan 
Published: February 2022

Abstract
Hybrid nanoflowers (hNFs), which are widely used in enzyme purification and catalytic activity applications, consist of organic and inorganic components. In this study, hNFs were synthesized in PBS buffer (pH: 5-9) by using different volume of Ascoseria mirabilis algae extract (0.65 ml, 1 ml and 1.65 ml) instead of expensive and difficult to obtain molecules such as enzymes and DNA for the synthesis process. According to the FE-SEM images, the morphology and diameters of the petals and NFs differ depend to the pH of the PBS buffer (synthesis did not occur at pH 5) and the volume of extract used. The catalytic and antioxidant properties of NFs synthesized with the used bioextract under optimum conditions (pH: 7.4, 1 ml extract) where a complete flower formation was observed were evaluated. The presence of Cu element and other components was detailed with EDX mapping. The peaks obtained by FT-IR analysis and the existence of functional groups that play a role in the synthesis are discussed. According to the results of the characterization tests, hNFs were synthesized by a cheap and eco-friendly with the coordination of Cu element and A. mirabilis extract in PBS buffer. Cu NFs exhibited peroxidase like catalytic activity against guaiacol, and antioxidant activity. The activities of hNFs is based on a Fenton-like mechanism. It is thought that this study will shed light on hNFs synthesis and catalytic activity application studies with bioextracts instead of biomolecules obtained by expensive and complex processes.
Constructing a prognostic immune-related lncRNA model for colon cancer
Authors: Lai Yu , Yang Lin , Li Xinyun , Huang Wei , Rao Xiangshu 
Published: February 2022

Abstract
Background: Colon cancer is a common digestive tract tumor. Although many gene prognostic indicators have been used to predict the prognosis of colon cancer patients, the accuracy of these prognostic indicators is still uncertain. Thus, it is necessary to construct a model for the prognostic analysis of colon cancer.Methods: We downloaded the original transcriptome data of colon cancer and performed a differential coexpression analysis of immune-related genes to obtain different immune-related long noncoding RNAs, which were paired as differentially expressed immune-related lncRNA pairs (DEirlncRNAPs). Then, the 1-year overall survival rate receiver operating characteristic (ROC) curve was calculated, and the Akaike information criterion value was evaluated to determine the maximum inflection point, which was used as the cutoff point to identify groups of colon cancer patients at high and low risk for death. Subsequently, the optimal prediction model was established. Finally, we used the patients’ survival times, clinicopathological features, tumor infiltrating immune cells, chemotherapy responses and immunosuppressive biomarkers to verify the DEirlncRNAP model.Results: Seventy-one DEirlncRNAPs were obtained to build the risk assessment model. The patients were divided into a high-risk group and a low-risk group according to the cutoff point. Then, the DEirlncRNAP model was verified using patient survival times, clinicopathological features, tumor-infiltrating immune cells, chemotherapy responses and immunosuppressive biomarkers.Conclusion: A new DEirlncRNAP model for predicting the prognosis of colon cancer patients was established, which could reveal new insights into the relationships of colon cancer with tumor-infiltrating immune cells and antitumor immunotherapy.
Evidence for paleo-continental margin and folding of relic fragment of the Bay of Bengal, northeast Indian subcontinent
Authors: Krishna Kolluru , Ismaiel M. , Rajan Abhimanyu 
Published: February 2022

Abstract
The processes of continental breakup and reassembling of landmasses are persistently contributing to reconfiguring the Earth’s surface. In addition, deltaic sediment deposition also reshapes the face of Earth by advancing shorelines towards the ocean. Here, we show a classic example of contiguous structures of paleo-continental margin on the eastern Indian Shield and an ancient fragment of the Bay of Bengal beneath Bangladesh. In the present work, we collated gravity anomaly data from the regions of Bangladesh and adjoined parts of the Indian shield and the northern Bay of Bengal for deciphering the nature and long-term behavior of rocks. The combined gravity anomaly map illustrates nearly N-S oriented alternate bands of about 200 km wide positive and negative anomalies in Bangladesh region, besides showing signatures of prominent geomorphic features such as Shillong Plateau, Sylhet Trough, Burmese Arc, etc. The crustal models reveal the presence of paleo-continental margin segments on eastern edge of the Singhbhum craton and south of the Shillong Plateau, approximately 200 km wide folded oceanic crust beneath Bangladesh, and low gradient (about 3.0° - 3.7°) bending of the subducting Indian plate beneath the Burmese platelet. The inferred continental margin was evolved after breakup occurred between the Rajmahal-Sylhet Line and continental fragments (Elan Bank and southern parts of the Kerguelen Plateau) at about 120 Ma, and then remained in marine conditions at least until the Oligocene-Miocene time (~23 Ma). Consequently, the sediments supplied by two great Himalayan rivers were excessively deposited on the margin and over an older fragment of the Bay of Bengal lithosphere for formation of Bengal Delta, leading to the delta progradation towards the ocean and creation of Bangladesh land. The oceanic crust beneath Bangladesh was folded with approximately 200 km wavelength as the compressive stress regime is locally operative due to the eastward moment of the Indian plate and westward propagation of the Indo-Burman Wedge.
The early impacts of COVID-19 on unpaid carers of people living with dementia: Part II of a mixed-methods systematic review
Authors: Giebel Clarissa , Talbot Catherine V , Wharton Emily , Lorenz-Dant Klara , Suarez-Gonzalez Aida , Cannon Jacqueline , Tetlow Hilary , Lion Katarzyna M , Thyrian Jochen Rene 
Published: February 2022

Abstract
Background

With a lack of existing comprehensive reviews, the aim of this mixed-methods systematic review was to synthesise the evidence on the early impacts of the pandemic on unpaid dementia carers across the globe.
Methods

This review was registered on PROSPERO [CDR42021248050]. PubMed, CINAHL, Embase, Scopus and Web of Science were searched from 2020 to July 2021. Studies were included if they reported on the different impacts of the pandemic on unpaid dementia carers aged 18+, with papers published in English, German, Polish, or Spanish. A number of research team members were involved in the selection of studies following PRISMA guidance.
Results

Thirty-six studies (43 papers) from 18 countries reported on the early impact of the pandemic on unpaid dementia carers. Impacts were noted on accessing care and support; carer burden; and well-being. Studies found that carers had limited access to care and support services, increased workload, enhanced feelings of isolation, and reduced wellbeing. All these negatives impacts were interlinked, with reductions in access to care and support increased carer’s unpaid caring tasks, removing any opportunities for temporary respite, and thus increasing carer burden and reducing mental well-being in many.
Conclusions

The needs of unpaid dementia carers appear to have increased during the pandemic, without adequate support provided. Policy initiatives need to enable better mental health support and formal care provision for unpaid carers and their relatives with dementia, whilst future research needs to explore the long-term implications of carer needs in light of care home restrictions and care delivery.
Longevity Compliance Scale
Authors: BELICE TAHIR 
Published: February 2022

Abstract
ObjectivesNine common characteristics of Blue Zone regions (Power 9) are having a natural movement pattern, having an ikigai (a reason for being), being able to cope with stress, being able to stop eating before being full, eating a predominantly plant-based diet, drinking wine regularly, having a sense of belonging, strong family ties and strong social bonds. In the present study, we investigated the relationship of Power 9 characteristics with age and gender using the 'Longevity Compliance Scale' that we have recently developed.MethodsData were collected by administering the online 'Longevity Compliance Scale' (nine questions of 5-point Likert scale type) to 490 participants. SPSS was used for calculations.ResultsCronbach's alpha value was found to be 0.763 (76.3%). Kaiser–Meyer–Olkin coefficient was 83%, and the factor analysis test provided high reliability (0.830 > 0.750). The total score was higher in female and elderly participants (Hedges' g: 0.046643, 95% Cls: 1.152-4.517, p:0.01).ConclusionWe found that longevity compliance developed predominantly in female and elderly participants. These results may vary across regions and cultures; thus, they cannot be generalized. However, it is crucial to demonstrate the effect of the nine common dominant points, which have been found after extensive studies conducted for many years. These nine points could be critical factors associated with longevity. It might provide us with tips to prolong our lives and help us stay healthy. Knowledge and experience accumulated as a result of aging, especially in women, may, instinctively or consciously, enhance compliance with the codes for longevity.
Optimizing Photoautotrophic Micropropagation Conditions for Ginger
Authors: Zhou Jie , Guo Fengling , Qi Chuangdong , Fu Jiaping , Xiao Ying , Wu Jinping 
Published: February 2022

Abstract
Ginger (Zingiber officinale), a member of the Zingiberaceae family, is a commonly available spice and medicinal plant. This study aimed to develop an alternative to conventional micropropagation using photomixotrophic micropropagation for ginger. Rootless ginger tissue culture plantlets were initiated in seedling trays grown in a chamber maintained under a 16 h/day photoperiod with 60 μE m-2 s-1 of cool white light from fluorescent lamps at 25 ± 2°C with an air relative humidity of 60 ± 5%. The photoautotrophic micropropagation of ginger was optimized using vermiculite + peat (1:1(v/v)) as substrate combined with MS + 0.5 mg/L NAA + 0.1% Metalaxyl-M·Hymexazol + 80% relative humidity (RH). After 40 days of culture, in-vitro ginger seedlings were successfully bred with a rooting rate of 100.0%. The seedlings could be directly transplanted to the field without the need for domestication and cultivation of conventional tissue culture seedlings.
A Novel Technique for Low Rectal Cancer: Pushing the Anus in Laparoscopic Radical Resection
Authors: Yang Rui , Yang Yuyi , Li Jianxin , Yang Qingqiang 
Published: February 2022

Abstract
Background: In sphincter-preserving surgery for low rectal cancer, it is significant to reduce the number of stapler cartridges and the incidence of anastomotic leakage. On this basis, we have developed a safer and more economical technique— pushing the anus in laparoscopic radical resection of low rectal cancer.Method: From January 2015 to July 2020, 213 consecutive patients with rectal cancer received laparoscopic radical surgery. For 151 of these patients, the surgeon used the stapler cartridges (Ethicon Intraluminal Linear Staplers EC60A, Ethicon, USA) to transect the edge of tumor of the rectum (Conventional Surgery Group). And for another 62 patients, besides applying the stapler cartridges, the surgeon had the assistants push the anus forward from the perineum during the process of transecting the rectum (Pushing the Anus Group). The postoperative outcomes and complications were compared between the two groups.Results: In terms of the number of the stapler cartridges, the Pushing the Anus Group was less than the Conventional Surgery Group (P<0.001). Moreover, the incidence of anastomotic leakage in the Pushing the Anus Group is lower than that in the Conventional Surgery Group (P=0.043). Conclusions: With pushing the anus forward during the process of transecting the rectum, the sphincter-preserving surgery can be performed more safely and economically.
Analisis Potensi Penerapan STEAM (Science, Technology, Engineering, Art, Mathematics) pada Kurikulum 2013 Bidang Studi Biologi SMA Kelas X
Authors: Halim Amalia Prabandani , Roshayanti Fenny 
Published: February 2022

STEAM (Science, Technology, Engineering, Art, Mathematics) adalah pendekatan pembelajaran yang menggabungkan 5 aspek yaitu sains, teknologi, teknik, seni, dan matematika yang dapat diterapkan dalam berbagai disiplin ilmu. Pendekatan STEAM sangat popular dimasa sekarang, sehingga dengan adanya penelitian ini diharapkan proses pembelajaran dapat dilaksakan menggunakan pendekatan STEAM. Penelitian ini bertujuan untuk mengetahui potensi penerapan STEAM pada Kurikulum 2013 Bidang Studi Biologi SMA Kelas X. Penelitian ini merupakan penelitian deskriptif kualitatif dengan subjek penelitian perangkat Kurikulum 2013 yaitu Kompetensi Dasar Bidang Studi Bioligi SMA Kelas X dan buku teks yang digunakan dalam proses pembelajaran. Hasil penelitian menunjukkan bahwa Kurikulum 2013 cukup berpotensi untuk diterapkan STEAM. Fakta tersebut dapat dikembangkan dan diintegrasikan dengan pendekatan STEAM sehingga mampu meningkatkan dan mengembangkan kemampuan yang dimiliki oleh setiap peserta didik.
Langmuir and Freundlich Equation Test on Methylene Blue Adsorption by Using Coconut Fiber Biosorbent
Authors: Baunsele Anselmus Boy , Missa Hildegardis - 
Published: February 2022

Methylene blue is a cationic dye often used for various industries. Methylene blue waste harms aquatic biota. Most ways have been done to reduce the methylene blue pollution that includes photocatalytic, electrochemical, and adsorption. This study aimed to reduce the impact of methylene blue pollution on the aquatic environment and to test the adsorption isothermal for methylene blue adsorption using coconut fiber. The abundant coconut fiber waste around the Kupang city was taken and prepared to be used as an adsorbent. The characterization results showed that the active groups in cellulose were clearly described on the FTIR spectra which showed the presence of O-H vibrations at 3296.35 cm-1, C-O vibrations were seen at 1267.23 and 1060.85 cm-1, at wavelengths 1608.63 cm-1 and 817.72 cm-1 there is a vibration from the C=C bond, while the C-H vibration appears at a wavelength of 2939.52 cm-1. The results of this research analysis showed that the adsorption of methylene blue according to the Langmuir isotherm indicated by the value of the equation of the straight line R2 being one and the direction of the curve depicting occurring the effective adsorption. The maximum adsorption capacity was 4.467 mg g-1 with the percentage of methylene blue adsorbed at 99.3%. Adsorption occurs chemically with an energy of 27.29 kJmol-1 so it can be determined that the adsorption occurs with a monolayer mechanism.
Screening for Inhibitors of Dihydrofolate Reductase using Pulsed Ultrafiltration Mass Spectrometry
Authors: Nikolic Dejan , van Breemen Richard B. 
Published: February 2022

:
A method of screening combinatorial libraries for inhibitors of eukaryotic dihydrofolate reductase has been developed using pulsed ultra-filtration electrospray mass spectrometry, which is a continuous-flow affinity separation system for extracting and identifying high affinity ligands in combinatorial libraries. In this application, pulsed ultrafiltration conditions were optimized for the isolation and identification of inhibitors of dihydrofolate reductase from a 22 compound library containing six known inhibitors of the enzyme including trimethoprim, aminopterin, methotrexate, pyrimethamine, folic acid, and folinic acid, and 16 compounds without known affinity. In order to optimize the screening method, sources of non-specific binding were identified and minimized. A significant source of non-specific binding for this set of library compounds was hydrophobic interaction with the surfaces of the ultrafiltration chamber. After affinity separation of bound (high affinity) versus free (low affinity) library compounds during pulsed ultrafiltration, receptor-bound ligands were released and eluted using either organic solvent or acidified mobile phase. Although 80% methanol easily disrupted the receptor-ligand complexes, organic solvent had the undesirable effect of releasing non-specifically bound compounds from the chamber and thereby increasing the background noise. Interference from non-specific binding was minimized by releasing bound ligands using a low pH mobile phase eluent instead of organic solvent. Under the conditions used, pulsed ultrafiltration mass spectrometry selectively identified the two library compounds with the highest affinity for dihydrofolate reductase, methotrexate and aminopterin.
Vitamin D Effect on the Severity of Chronic Pelvic Pain in Patients with Ovarian Endometriosis Combined with Pelvic Inflammatory Diseases
Authors: Bihun Ruslana 
Published: February 2022

Numerous publications in recent years indicate the impact of vitamin D deficiency on the course and severity of a number of gynecological diseases, including endometrial disease. As this transmitter is associated with immune disorders, its involvement in local immunosuppression in the progression of the systemic inflammatory response in the case of endometrial disease is not excluded. Considering the pathogenetic links between vitamin D metabolism and the severity of pelvic pain, it became appropriate to study the relationship between this parameter and the intensity of pelvic pain in patients with ovarian endometriomas associated with chronic pelvic inflammatory diseases, which was the objective of this research. Materials and methods of research. The study included 90 patients with ovarian endometriosis aged 20 to 41 years. The main group consisted of 44 patients with endometrioma combined with pelvic inflammatory diseases; the comparison group consisted of 46 patients with isolated ovarian endometrioma. The control group included 30 relatively healthy women without gynecological and somatic pathology. At the initial stage, the level of 25-hydroxyvitamin-D3 (25 (OH) D) was determined in patients of the three groups by mass spectrometry using standard methods. Analysis and discussion of research results. Patients with ovarian endometrial cysts on the background of pelvic inflammatory disease were statistically significantly older compared with the group of isolated endometriomas; more than half of the observations were patients aged 36-40 years (p<0.05). Laparoscopic diagnostic and therapeutic techniques allowed to present objective signs of combination of ovarian endometrioma with manifestations of external genital endometriosis: severe manifestations of external genital endometriosis were diagnosed in 31.8% vs. 15.21% in the comparison group. Depending on the severity of the pain syndrome according to VAS, patients of the main group were stratified as follows: 22.7% noted pelvic pain of low intensity, 40.9% – moderate pain, 25.0% – severe pain; in the comparison group there were no manifestations of severe pelvic pain. The combination of pelvic inflammatory disease with ovarian endometriosis was accompanied by an increase in the share of vitamin D deficiency – in 38.64% and the share of severe pain – in 25.0%.
Conclusion. A significantly moderate inverse correlation was found between the verification of the manifestations of external genital endometriosis and the level of vitamin D in the blood serum. The obtained data dictated the need to assess the therapeutic potential of vitamin D in combination with nonsteroidal anti-inflammatory drugs to enhance the analgesic effect and reduce the drug load.