bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential secondary metabolite analysis of soil Streptomyces sp. GMR22 and antibacterial assay on Porphyromonas gingivalis ATCC 33277
Authors: Nirwati Hera , Damayanti Ema , Sholikhah Eti Nurwening , Mustofa . , Widada Jaka 
Published: May 2022

Infectious diseases caused by oral pathogenic bacteria are currently a serious problem due to the increasing incidence of antimicrobial resistance. Streptomyces sp. GMR22, a soil actinobacterium which has large-genome size. In previous studies, it was known to have antifungal, and antibiofilm activity on Candida albicans. However, its antibacterial activity on oral pathogenic bacterium, Porphyromonas gingivalis is not clear. This study aimed to identify potential active compound based on genome mining analysis and to evaluate the antibacterial activity of GMR22 extract on P. gingivalis ATCC 33277. Potential active compounds and biosynthesis gene clusters were analysis using antiSMASH version 5. Antibacterial activity assay was carried out by the microdilution method on P. gingivalis ATCC 33277. Based on genome mining analysis polyketide synthase (PKS), the Streptomyces sp. GMR22 is the abundant BGCs (35%) and has large-predicted compounds which have antibiotic-antibacterial activity (22.9%). On antibacterial assay, chloroform extract of GMR22 at 7.8 – 62.5 µg/mL has high antibacterial activity on P. gingivalis compared to other extracts. Soil Streptomyces sp. GMR22 bacterium has biotechnological potential to produce active compounds for antibacterial.
ANALISIS MANAJEMEN WAKTU PADA IBU BEKERJA
Authors: Agiani Preista , Nursetiawati Sitti , Muhariyati Metty 
Published: May 2022

AbstrakPenelitian ini bertujuan untuk menganalisis manajemen waktu pada ibu bekerja terutama dalam membagi waktu antara pekerjaan rumah tangga dengan pekerjaan publik. Metode yang digunakan dalam penelitian ini ialah metode deskriptif. Informan dalam penelitian ini berjumlah 7 orang wanita yang bekerja sebagai guru SMP Negeri di Kecamatan Cibinong. Teknik pengambilan data yang digunakan yaitu wawancara, observasi dan dokumentasi. Data dianalisis dengan metode deskriptif dan teknik pemeriksaan keabsahan data menggunakan member check. Hasil penelitian ini menunjukkan bahwa manajemen waktu pada ibu bekerja yang berprofesi sebagai guru SMP Negeri di Kecamatan Cibinong sudah cukup baik. Kegiatan ibu bekerja pada hari senin hingga minggu dapat disimpulkan rata-rata per-24 jam untuk pekerjaan rumah tangga (domestik) persentasenya sebesar 22 persen yaitu 5,28 jam, pekerjaan publik (guru) persentasenya 31 persen yaitu 7,44 jam, waktu luang persentasenya sebesar 12 persen yaitu 2,88 jam, dan pekerjaan/kebutuhan pribadi persentasenya sebesar 35 persen yaitu 8,4 jam.Kata kunci: manajemen waktu, ibu bekerja, guru
AbstractThis study aims to analyze time management of working mothers, especially in dividing her time between housework with public employment. The method used in this research is descriptive method. Informants in this study amounted to 7 persons, namely women who worked as a teacher in the district of Cibinong Junior High School. Data collection techniques used were interviews, observation and documentation. Data were analyzed with descriptive methods and techniques of data validity checking using the member check. The results showed that the time management in working mother who works as a junior high school teacher in the district of Cibinong is good enough. Activity mother worked on Monday through Sunday can be summed average per-24 hours to housework (domestic) percentage of 22 percent (5.28 hours), public employment (as a teacher) the percentage is 31 percent (7.44 hours), free time percentage of 12 percent (2.88 hours), and work/personal needs percentage of 35 percent (8.4 hours).Keywords: management time, mother worked, teachers
PROPOLIS AND BEE VENOM IN DIABETIC WOUNDS; A POTENTIAL APPROACH THAT WARRANTS CLINICAL INVESTIGATION
Authors: Al-waili Noori , Hozzein Wael N. , Badr Gamal , Al-Ghamdi Ahmed , Al-Waili Hamza , Salom Khelod , Al-Waili Thia 
Published: May 2022

Background: Wound healing in diabetes mellitus is a complex multi-stage process that requires the proper function of multiple systems. The
mechanisms of impaired wound healing of diabetic wounds are still poorly understood. Therefore, various interventions are being used for wound
management without great success. Bee products have various properties that make them an important addition to the diabetic wound management.
Methods: This review summarized previous and recently published papers of the effects of two bee products, propolis and bee venom, on the wound
healing. The main results were obtained from preclinical experimentation.
Results: Diabetes mellitus compromises immune system, increases infections, impairs wound healing, and affects cells and factors involved in the
wound healing. There is an increasing interest in natural products in modern medicine as part of disease management. Bee products are natural
substances that others and we have explored some of their biological activities and applications in the treatment of various diseases. Some of these
products are bee venom and propolis. These products have analgesic, antioxidant, antimicrobial, and anti-inflammatory properties. In addition, both
propolis and bee venom contain considerable amounts of antioxidants that have a great role in accelerating wound healing.
Conclusion: There is sound rationality and scientific data for using propolis and bee venom in diabetic wound healing. We believe that topical
application of propolis in addition to bee venom might have a place in repairing damaged tissues and accelerating the healing of diabetic wounds.
TREATMENT OF BOVINE SCHISTOSOMIASIS WITH MEDICINAL PLANT VERONIA ANTHELMINTICA (KALIZIRI), AN ALTERNATIVE APPROACH
Authors: Niaz Sadaf , Akhtar Tanveer , Shams Sumaira , AbdEl-Salam Naser M. , Ayaz Sultan , Ullah Riaz , Bibi Safia , Hussain Iqbal , Ahmad Shabir 
Published: May 2022

Background: Veronia anthelmintica (Kaliziri) is a medicinally very important plant as being reported for remarkable therapeutic potential. The
present research evaluates its antischistosomal activity.
Methods and Materials: 54 buffaloes naturally infected with Schistosomiasis were used for anthelmintic trails. All animals for the study were
tagged so as to distinguish from others. The animals were checked up on routine basis by a veterinary doctor. Preparation of samples of herbal
drug and experiment was design according to available literature.
Results: During present studies, buffalos naturally infected with Schistosomiasis were treated with Veronia anthelmintica (Kaliziri), with dose
75 mg/kg, 150 mg/kg and 225 mg/kg body weight respectively, and their impact on various parameters like eggs reduction, milk production,
weight gain and feed intake was noted. Furthermore, their efficiency (%) was compared with standard drug “Praziquantel” (allopathic drug) at
“10mg/Kg” body weight dose level. The effectiveness (%) of “Praziquantel” was noted as hundred after first dose, while the effectiveness for the
herbal drug get to this level after injecting of second dose of “225mg/kg body weight”. Statistically significant (P
THERAPEUTIC POTENTIAL OF PEGNUM HARMELA AGAINST SCHISTOSOMA BOVIS IN BUFFALOES
Authors: Niaz Sadaf , Akhter Tanveer , AbdEl-Salam Naser M. , Ayaz Sultan , Shams Sumaira , Ullah Riaz , Hussain Hidayat , Bibi Safia 
Published: May 2022

Background: Peganum harmala have many biological and pharmacological activities such as antifungal, antibacterial, analgesic and antiinflammatory.
The present study was carried out to evaluate the antischistosomal activities of Peganum harmala with special reference to bovine
schistosomiasis in naturally infected buffaloes.
Methods and Materials: A total of 54 naturally infected buffaloes with “Schistosomiasis” of ages around five years were used for anthelmintic
studies. All of the buffaloes were properly marked so as to make a distinction from one other. A doctor of veterinary was available for daily checkup.
Samples prepared according to the available methods in literature.
Results: In the present study, naturally infected buffaloes with Schistosoma bovis were treated with Peganum harmala (Harmal, seed), with dose 75
mg/kg, 150 mg/kg and 225 mg/kg body weight respectively, and their impact on various parameters like eggs reduction, milk production, weight gain
and feed intake was noted. Furthermore, their efficiency (%) was evaluated with “Praziquantel” (allopathic drug) at “10mg/Kg body weight” dose
level. After first dose of “Praziquantel” hundred percent effectiveness was noted, while the same result obtained for herbal drug after giving second
dose of “225mg/kg body weight”. To evaluate the effect of herbal medicines on protein portions, sera of treated and control buffaloes were also
investigated.
Conclusion: From present findings, it is concluded that the herb Peganum harmala can easily replace Praziquantal (PZQ) with almost same
efficiency (%). Furthermore, the herb is easily available at cheap price at local market and it will be good for the economy of Pakistan
WOUND HEALING POTENTIAL OF NAUCLEA LATIFOLIA AND MANIHOT ESCULENTA LEAF EXTRACTS IN TYPE 1DIABETIC RATS
Authors: Ajayi Ebenezer Idowu O. , Popoola Gbemisola , Ojediran Edward 
Published: May 2022

Background: This study was designed to ascertain this claim and to investigate the possible mechanism of action in relation to their phytochemical
contents, using crude extracts of N. latifolia and M. esculenta leaves on topical wound in a rat model of Type 1 diabetes.
Methods and Materials: The leaves were air-dried under shade and subjected to cold extraction in 95% ethanol. Normoglycaemic male Wistar rats
were subjected to 2-month high-energy diet/ fat emulsion manipulation and injected with alloxan (150 mg/kg BW/day, i. p.). Fasting plasma glucose
was determined after 7 days and rats with values exceeding 200 mg/dl were selected. 16 diabetic rats (120 – 180 g) were randomly assigned to four
groups of four animals each. Wound was inflicted on the back of each rat by excision method. The crude extracts were topically applied over a period
of 21 days. The scar tissues were removed at 7 days interval for collagen quantification and wound margin reduction was also monitored using
tracing paper.
Results: The crude extracts showed efficient wound healing activity as revealed by increased collagen content (scar tissues versus fresh wound
tissues ab initio). Percentage wound closure also progressed significantly (p< 0.05) upon topical application of the extracts.
Conclusion: The crude extract of N. latifolia leaves has proven to be more potent than that of M. esculenta in wound healing in Type I diabetic rats.
It stimulated wound contraction and collagen formation, making it a promising natural product for further screening in search of new chemical
entities that can be useful in diabetic wound management.
ANALGESIC, ANTI-INFLAMMATORY AND ANTIPYRETIC ACTIVITIES OF THE AQUEOUS EXTRACT OF GERANIUM CAROLINIANUM L
Authors: Li Yuan , Ye Ying , Wang Su-Juan , Xia Wei , Rahman Khalid , Yue Wei , Zhang Hong 
Published: May 2022

Background: Geranium carolinianum L. (Geraniaceae) is widely used for a variety of diseases including herpetic keratitis, eczema,
rheumatalgia etc. However, there is lack of relevant scientific research.
Materials and Methods: GCE (125, 250, 500mg/kg body weight) was evaluated for its pharmacological properties by using the acetic
acid-induced writhing test, the hot plate test and the fresh egg white-induced paw edema in rats. The dimethylbenzene-induced mouse
inflammation model and the lipopolysaccharide (LPS)-induced rat fever model were employed and the acute toxicity of GCE was also
assessed.
Results: The Geranium carolinianum aqueous extract (GCE) significantly inhibited the writhing responses in mice, increased reaction time of
mice in the hot plate test, and suppressed the fresh egg white-induced paw edema in rats and the dimethylbenzene-induced ear edema in mice
whilst attenuating LPS-induced fever in rats in a dose dependent manner. Furthermore, no deaths were observed when mice were orally
administered GCE up to 14 g/kg body weight (approximately 553 times of clinical dose).
Conclusions: GCE possesses analgesic, anti-inflammatory and antipyretic activities and is non-toxic at the doses used. The results of this
study support the clinical use and effectiveness of Geranium carolinianum as an analgesic, anti-inflammatory and antipyretic agent in folk
medicine.
MEDICAL STUDENTS' PERCEPTIONS OF COMPLEMENTARY AND ALTERNATIVE MEDICINE THERAPIES: A PRE- AND POST-EXPOSURE SURVEY IN MAJMAAH UNIVERSITY, SAUDI ARABIA
Authors: Al Mansour Mohammed Abdullah , Al-Bedah Abdullah M.N. , Elsubai Ibrahim S. , Al- Rukban Mohammed Othman , Mohamed Elsadig Yousif , El Olemy Ahmed Tawfik , Khalil FCM Asim A.H. , Khalil Mohamed K. M. , Alqaed Meshari Saleh , Almudaiheem Abdullah , Mahmoud Waqas Sami , Medani Khalid Altohami , Ali Gazzaffi Ibrahim Mahjoub , Qureshi Naseem A. 
Published: May 2022

Background: Evidently, Complementary and Alternative Medicine (CAM) is increasingly a recognized medical practice that efficiently uses
multiple treatment therapies and techniques in promoting the health and wellbeing of people as well as preventing and managing a variety of
human disorders. Research in CAM, which courses exposure to diverse healthcare professionals, is important from many perspectives including
improvement in teaching skills of faculty, enhancing capacity building, and innovative curriculum development. This pre- and post-design crosssectional
study aimed to assess perceptions, training needs, personal usage, use in office practice, and knowledge of two batches of medical
students toward CAM therapies in Majmaah University, Saudi Arabia.
Materials and Methods: The second year medical students of the first (year 2012-13) and second (year 2013-2014) batch [n=26 & 39,
respectively] were selected for this study. A reliable 16-item self-administered questionnaire was distributed among all students for answering
before and after the 48-hour specific 19 CAM therapies course, in terms of CAM therapies are clearly conventional or alternative, training needs,
effectiveness, personal use, use in practice, management of two clinical cases by CAM or conventional therapies, and views about which
evidence based approach strongly support individual CAM modalities.
Results: Medical students' knowledge and perceptions of CAM therapies significantly improved across some sub-items of CAM questionnaire
with a positive trend in the rest of its items including their views about CAM therapies, need for further training, personal use of therapies and
advising patients regarding CAM practices strongly supported by randomized clinical controlled trials and published case studies.
Conclusion: CAM course tends to have positive impact on the knowledge and perceptions of medical students, in addition to need for further
training, and personal use and use of CAM therapies in practice in line with strong evidence-based data regarding therapeutic efficacy. The
preliminary results of this study call for further research in specific CAM modalities with a larger sample in academic settings across the nation.
SELENIUM ALONE OR IN COMBINATION WITH LYCOPENE MODIFIES LIVER METABOLIZING ENZYMES AGAINST GALACTOSAMINE – A TIME BOUND STUDY
Authors: Khan Jehan A. , Moselhy Said S. 
Published: May 2022

Background: Hepatitis is one of the major public health problems worldwide. This study was designed to evaluate the potential
hepatoprotective effects of lycopene (Lyco) and selenium (Se) against galactosamine (Gala) induced hepatitis in rats.
Materials and Methods: Seventy five (75) male albino rats were grouped into five of fifteen rats each. GP 1: Control. Animals in Groups (II-V)
were injected i.p with Gala (300 mg/kg b.w daily) for 5 days. GP III: Rats were orally pretreated with Lyco (15 mg/kg b.w). GP IV: rats were
pretreated orally sodium selenite (0.1 mg/kg b.w) by gavages. GP V: Rats treated with both Lyco and Se. The treatment was continuous for 30
days.
Results: The levels of serum inflammatory markers interleukine-6 (IL-6), tumor necrosis factor TNF- α, nitric oxide (NO) and malondialdhyde
(MDA) were markedly elevated in rats injected with Gala compared with control group. Administration of Lyco combined with Se reversed
these effects and significantly reduced the levels of these markers (p
A BIBLIOMETRIC STUDY ON CHINESE HERBAL MEDICINE TREATMENT OF CARDIOVASCULAR DISEASES
Authors: Huang Youliang , Deng Qingqiong , Zhang Juan , Sajid Ali , Shang Xiangang , Zhou Mingquan 
Published: May 2022

Background: The aims of this study are to evaluate and summarize the scientific production in the field of Chinese herbal medicine (CHM)
treatment of cardiovascular disease (CVD).
Methods: A systematic bibliometric search was performed based on the PubMed database covering relative publications between January 1,
1995, and December 31, 2014. Core of the search strategy include the key word cardiovascular disease and the Medical Subject Heading
Chinese herbal medicine. The number, type, country, major journals, and research focus of articles were analyzed in accordance with
bibliometrics methodologies.
Results: The retrieve results were analyzed and described in the form of texts, tables, and graphs. A total of 3,826 articles were identified,
40.28% of which were original articles. All articles were from 50 countries/territories. China was ranked first with 2,258 articles, followed by
Japan with 173 articles. 665 articles were published by Zhongguo Zhong Xi Yi Jie He Za Zhi.
Conclusion: The publication activity of literature has grown rapidly in the past 20 years, indicating enhanced attention and increasing research
input to CHM treatment of CVD. Owing to its great advances in scientific studies, CHM will continue to play an important role in medical
research.
ANTIULCEROGENIC BENEFITS OF HERBAL INGREDIENTS IN ETHANOL-INDUCED ANIMAL MODELS
Authors: Bi Weiping , Man Huibin , Hu Lizhi , Man Mao-Qiang 
Published: May 2022

pathogenesis of gastric ulcer. Although therapeutic approaches are widely available, preventive regimens are limited. Numerous studies
have demonstrated that herbal medicines display preventive benefit in the development of ethanol-induced gastric ulcers in both rat and
mouse models. The preventive efficacy of herbal medicines on the development of ethanol-induced gastric ulcers is comparable or
superior to histamine receptor 2 antagonists. But herbal medicines have fewer side effects.
Materials and Methods: Keywords including gastric ulcer, herbal medicines, prevention and natural ingredients were used to search
on http://www.ncbi.nlm.nih.gov/pubmed. The search was performed on January 12, 2014. Only articles published in English were used
in this review.
Result and Conclusion: According to the mechanisms of their action and pathogenesis of gastric ulcer, combination of certain herbal
medicines could be a valuable alternative to prevent the development of gastric ulcer, particularly for the subjects who are at a higher risk
of developing gastric ulcer.
ANTI-OBESITY POTENTIALS OF AQUEOUS AND METHANOL EXTRACTS OF Vernonia amygdalina DEL. LEAVES IN HIGH-FAT DIET FED RATS
Authors: Egedigwe Chima Agatha , Ejike Chukwunonso E.C.C. , Ijeh Ifeoma I. , Herbert Udo , Onwuka Gregory I. , Asumugha Victoria U. 
Published: May 2022

Background: Obesity is currently a global epidemic. Conventional treatments have not been very satisfactory to patients, warranting
a search for alternative therapeutic options that are natural, safe and affordable. This study therefore investigated the anti-obesity
potentials of aqueous and methanol extracts of Vernonia amygdalina Del (AEVA and MEVA respectively) in a rat model in which
obesity was induced using a high-fat diet.
Materials and Methods: Forty two Wistar rats were randomised into 7 groups of 6 rats each. One group served as the Normal
Control group and obesity was induced in the other 6 groups. One of the 6 groups each served as Positive Control and Negative
Control while the 4 test groups were designated AEVA100, AEVA500, MEVA50 and MEVA200, respectively. The study lasted for
12 weeks after which standard protocols were followed for all analyses and determinations.
Results: The results show that both AEVA and MEVA at the tested concentrations resulted in significant (P < 0.05) weight loss
(without affecting internal organs negatively), and significant (P < 0.05) improvement in some metabolic markers of obesity in the
test rats compared to the negative control rats. MEVA 200 had the greatest anti-obesity effect while MEVA 50 was the least
effective. All the test extracts compared well with Orlistat used as the positive control drug on all counts.
Conclusion: The observed weight-loss benefits of AEVA and MEVA are attributable to the rich milieu of phytochemicals found in
Vernonia amygdalina Del. Further studies to unlock the mechanisms through which the observed weight loss is mediated are
warranted.
INSULIN-MIMETIC ACTIVITY OF STEVIOSIDE ON DIABETIC RATS: BIOCHEMICAL, MOLECULAR AND HISTOPATHOLOGICAL STUDY
Authors: Saleh Osama M. , Awad Nabil S. , Soliman Mohamed M. , Mansour Ahmed A. , Nassan Mohammed A. 
Published: May 2022

Background: Stevioside has been used as a medication for reducing glucose levels in diabetic patients. The exact mode of action is still unclear. Therefore, the current study outlines the molecular and biological roles of stevioside in treatment of diabetes.
Materials and Methods: induced diabetic male wistar rats treated with stivioside and metformin as therapy for diabetic rats. Biochemical, molecular and histopathological studies have been done to evaluate the therapeutic effect of stevioside on minimizing levels of glucose in diabetic rats.
Results: Stevioside administration normalized kidney and liver biomarkers, restored alterations in antioxidants activity and lipid profiles. Moreover, stevioside increased insulin and leptin secretion that are decreased in diabetic rats to the normal levels.For mRNA expression, stevioside up-regulated the expressions of PK and IRS-1 genes which are down-regulated in diabetic rats, and was very effective in down-regulation of CPT-1 mRNA expression. At the cellular levels; stevioside normalized the histopathological changes induced in pancreas.
Conclusion: Stevioside has insulin like effects and it is useful for diabetic patient’s therapy.
ACUTE AND SUB-CHRONIC ORAL TOXICITY STUDIES OF METHANOL EXTRACT OF CLINACANTHUS NUTANS IN MICE
Authors: Zakaria Zainul Amiruddin , Abdul Rahim Mohammad Hafiz , Mohtarrudin Norhafizah , Abdul Kadir Arifah , Cheema Manraj Singh , Ahmad Zuraini , Mooi Ching Siew , Md. Tohid Siti Farah 
Published: May 2022

Background: Clinacanthus nutans (C. nutans) Lindau (family Acanthaceae) is a shrub widely cultivated in the South East Asia region,
including Malaysia. It has been traditionally used for treatment of various ailments including pain-mediated diseases. Various
pharmacological activities of C. nutans have been reported except for its pain-relieving activity. This study was performed to evaluate the
acute and sub-chronic oral toxicity of the methanol extract of Clinacanthus nutans (MECN) in male and female mice.
Methods: In the acute toxicity study, a single dose of 5000 mg kg-1 of body weight MECN was administered orally, and was monitored
for 14 consecutive days. In the sub-chronic toxicity study, the MECN was administered orally at doses of 50, 500, and 2500 mg kg-1 day-1
for 28 days. Results: Results showed that there were no clinical signs of toxicity, mortality and body weight changes in both acute and
subchronic toxicity studies. There were no significant differences in relative organ weight, haematological parameters, and biochemical
parameters; except for the creatinine level, which indicated significant (p