bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using whole-genome sequencing to characterize clinically significant blood groups among healthy older Australians
Authors: Jadhao Sudhir , Davison Candice , Roulis Eileen V. , Lee Simon , Lacaze Paul , Riaz Moeen , McNeil John J. , Thomas David M. , Pecheniuk Natalie M. , Hyland Catherine A. , Flower Robert L. , Nagaraj Shivashankar H. 
Published: April 2022

AbstractThere have been no comprehensive studies of a full range of blood group polymorphisms within the Australian population. This problem is compounded by the absence of any databases carrying genomic information on chronically transfused patients and low frequency blood group antigens in Australia. Here, we use RBCeq, a web server–based blood group genotyping software, to identify unique blood group variants among Australians and compare the variation detected vs global data. Whole-genome sequencing data were analyzed for 2796 healthy older Australians from the Medical Genome Reference Bank and compared with data from 1000 Genomes phase 3 (1KGP3) databases comprising 661 African, 347 American, 503 European, 504 East Asian, and 489 South Asian participants. There were 661 rare variants detected in this Australian sample population, including 9 variants that had clinical associations. Notably, we identified 80 variants that were computationally predicted to be novel and deleterious. No clinically significant rare or novel variants were found associated with the genetically complex ABO blood group system. For the Rh blood group system, 2 novel and 15 rare variants were found. Our detailed blood group profiling results provide a starting point for the creation of an Australian blood group variant database.
Identification of sentinel plant species for evaluating phytotoxicity of veterinary antibiotics in Mediterranean Europe
Authors: García Rosa M. , Martínez-Fernández Jesús , Rodríguez Antonio , de la Torre Ana 
Published: April 2022

Abstract
Background
Antibiotics used to treat livestock species enter agricultural fields when they are excreted by grazing animals or are present in manure that is added to fields as fertiliser. In the European Union, the potential effects of such antibiotics on terrestrial plants must be evaluated following the standardised OECD 208 method, which specifies the crop and wild species that should serve as “sentinels” for assessing antibiotic exposure. The present study aimed to compare this approved list of sentinel species against crop and wild plant species actually present in agricultural and pasture lands in Mediterranean Europe in order to identify the most appropriate sentinel plants for the region. The study focused on Spain as a region representative of Mediterranean Europe. Georeferenced layers for wild plant species and cultivated areas (crops), livestock density and land cover were combined, and then selection criteria were applied, leading to the identification of sentinel crop and wild species for crop land and pasture scenarios.

Results
In the crop land scenario, the sentinel crop species were barley (Hordeum vulgare L.), wheat (Triticum spp.), corn (Zea mays L), sunflower (Helianthus annuus L.), dried pea (Pisum sativum L.), alfalfa (Medicago sativa L.), vetch (Vicia sativa L.), oilseed rape (Brassica napus L.) and sugar beet (Saccharum officinarum L.), all of them listed in the OECD 208 method with the exception of alfalfa; the sentinel wild species were Papaver rhoeas L., Galium aparine L. and Chenopodium album L. In the pasture scenario, sentinel wild species were Bromus tectorum L., Agrostis capillaris L., Trifolium pratense L., Lotus corniculatus L. and Galium aparine L. The following common weed species in field boundaries or in pasture lands also emerged as potential sentinel species for risk assessment, even though they are not listed in the OECD 208 method: Sonchus oleraceus L., Avena sterilis L., Dactylis glomerata L., Hordeum murinum L. and Lolium rigidum Gaudin.

Conclusions
The sentinel species identified in this study may be useful in risk assessment procedures covering the Mediterranean Europe. The method developed for this study could be applied to identify sentinel species for other representative agroclimatic regions in Europe (such as Atlantic and Continental).
Intermittent fasting induces chronic changes in the hepatic gene expression of Red Jungle Fowl (Gallus gallus)
Authors: Lindholm Caroline , Batakis Petros , Altimiras Jordi , Lees John 
Published: April 2022

Abstract
Background
Intermittent fasting (IF), the implementation of fasting periods of at least 12 consecutive hours on a daily to weekly basis, has received a lot of attention in recent years for imparting the life-prolonging and health-promoting effects of caloric restriction with no or only moderate actual restriction of caloric intake. IF is also widely practiced in the rearing of broiler breeders, the parent stock of meat-type chickens, who require strict feed restriction regimens to prevent the serious health problems associated with their intense appetites. Although intermittent fasting has been extensively used in this context to reduce feed competition and its resulting stress, the potential of IF in chickens as an alternative and complementary model to rodents has received less investigation. In both mammals and birds, the liver is a key component of the metabolic response to IF, responding to variations in energy balance. Here we use a microarray analysis to examine the liver transcriptomics of wild-type Red Jungle Fowl chickens fed either ad libitum, chronically restricted to around 70% of ad libitum daily or intermittently fasted (IF) on a 2:1 (2 days fed, 1 day fasted) schedule without actual caloric restriction. As red junglefowl are ancestral to domestic chicken breeds, these data serve as a baseline to which existing and future transcriptomic results from farmed birds such as broiler breeders can be compared.

Results
We find large effects of feeding regimen on liver transcriptomics, with most of the affected genes relating to energy metabolism. A cluster analysis shows that IF is associated with large and reciprocal changes in genes related to lipid and carbohydrate metabolism, but also chronic changes in genes related to amino acid metabolism (generally down-regulated) and cell cycle progression (generally up-regulated). The overall transcription pattern appears to be one of promoting high proliferative plasticity in response to fluctuations in available energy substrates. A small number of inflammation-related genes also show chronically changed expression profiles, as does one circadian rhythm gene.

Conclusions
The increase in proliferative potential suggested by the gene expression changes reported here indicates that birds and mammals respond similarly to intermittent fasting practices. Our findings therefore suggest that the health benefits of periodic caloric restriction are ubiquitous and not restricted to mammals alone. Whether a common fundamental mechanism, for example involving leptin, underpins these benefits remains to be elucidated.
Blood pressure profile of primary school children in Eastern Cape province, South Africa: prevalence and risk factors
Authors: Gomwe Howard , Seekoe Eunice , Lyoka Philemon , Marange Chioneso Show 
Published: April 2022

Abstract

Background
The problem of cardiovascular diseases and lack of adequate information about the blood pressure profiles among children in South Africa has enormous consequences for public health and the general well-being of communities.

Aim
The aim of this study is to determine the blood pressure profiles and associated risk factors of primary school children in South Africa.

Methods
A cross sectional study was conducted among 876 children aged 9 to 14 years from 18 randomly selected schools in the Eastern Cape province of South Africa. Standardised blood pressure measuring instruments were used and an average of three readings was considered. Blood pressure status was classified according to the percentiles of systolic blood pressure (SBP).

Results
The overall prevalence of hypertension was 5.2% and pre-hypertension was 18.5% while normal blood pressure was 76.3%. The multilevel binary logistic regression’s crude and adjusted analysis revealed that increase in age was significantly associated with elevated BP in children ([crude OR = 1.17 [1.05 – 1.29] and [adjusted OR = 1.12 [1.01 – 1.25]). In addition, increase in BMI was significantly associated with elevated BP in children ([crude OR = 1.08 [1.04 – 1.12] and [adjusted OR = 1.06 [1.02 – 1.11]). There was no statistically significant association between elevated BP and gender for both the univariate and multivariate models. There was also no statistical significant risk for elevated BP associated with place of residence.

Conclusions
In this sampled population the established proportion of primary school children with elevated BP is of great concern. In addition, older children and those with high BMI (that is, overweight/obesity) were associated with elevated BP. Interventions towards promoting healthy lifestyles among school learners is a necessity if we are to prevent cardiovascular diseases.
Is nutritional functional diversity in the rural food and nutrition system associated with food security and nutrient adequacy? A case study of rural areas of Zahedan district, Iran
Authors: Sheikhi Mahdieh , Omidvar Nasrin , Tabatabaei Seyed Mehdi , Eini-Zinab Hassan 
Published: April 2022

Abstract
Background
An overlooked problem in food and nutrition system analysis is assuring adequate diversity for a healthy diet. Little is known about nutrient diversity in food and nutrition systems and how it transmits to dietary diversity. Nutritional functional diversity (NFD) is a metric that describes diversity in providing nutrients from farm to market and the consumption level. The objective of this study is to determine the NFD score at different stages of the rural food and nutrition system, including household’s agricultural and home production, domestic food processing, purchased food, and diet. It also aims to explore the association between NFD and nutrient adequacy, food security, and anthropometric indicators.

Methods
A cross-sectional study was conducted on 321 households in 6 villages of Zahedan district. The NFD score was measured at three subsystems (production, processing, and consumption) of the food and nutrition system. Household food security, mean adequacy ratio (MAR), and anthropometrics of the household’s head were measured to assess the association between NFD and food and nutrition indicators. Linear and bivariate statistical techniques were applied to study the associations between variables.

Results
In the rural food and nutrition system, the food purchased from the city plays the main role in the households NFD score. Their contribution to total NFD was twice that of the food items purchased from the village. The NFD score of homestead production and households food processing was found to be five times less than those of food purchased from cities. The food insecure households had significantly lower NFD scores for food purchased from the city and higher NFD scores for purchased food items from the rural market and native wild vegetable consumption. A strong and positive relationship was observed between NFD of food items purchased from the city and households’MAR. No significant association was found between the NFD score of homestead production, processing, and dependent variables, i.e. food insecurity, MAR, and household head anthropometrics.

Conclusion
NFD score, as a relatively new metric, could help in determining diversity from farm to diet and identifying the gaps to plan appropriate interventions for improving diversity in the local food system.
Level playing field: young males, masculinity and mental wellbeing through sport
Authors: Drummond Murray , Wadham Ben , Prichard Ivanka , Elliott Sam , Drummond Claire , Crossman Sarah 
Published: April 2022

AbstractIn Australia, and throughout the world, it is evident that the mental health and wellbeing of young males aged 15–24, is not a priority. In Australia suicide is the leading cause of death in people aged 15–24 years and 75% are male (Australian Institute of Health and Welfare. Australian hospital statistics 2011–12, 2021). It is clear young males as well as those who identify as indigenous or LGBTIQ are at risk groups with respect to self arm and suicide (Drummond, MJN, et al. 2019). It is the transition period from adolescence to adluthood that is of particular concern. Often young males must pass through this phase of life with minimal guidance or direction and without a “safe space” where they can attain emotional support. Sport is a significant part of boys’ and young males' lives and offers that “safe space”. Sport can play a substantial role in protective mental health through socialization and engagement in a socially endorsed activity that seemingly has far more positive outcomes than negative ones. This research is underpinned by both quantitative and qualitative research with young males involved masculinised sporting clubs. Its aim is to provide insights into how we can create safe spaces for males and influence positive forms of masculinities that can enhance mental health promotion among young males. This mixed methods research explores issues around mental health in young males involved across two male dominated sporting codes in Australian (Australian football and cricket). Surveys and interviews with young males age 15–24 as well as parents, coaches and key stakeholders underpin this research. While the data is designed to assist in the development of educational resources for males to influence positive forms of masculinities that can enhance mental health promotion among young males involved in these sporting codes, this paper reports on the exploratory nature of the data and raises important issues emerging among young males with respect to mental health and the role of the sporting club.
Intraparenchymal breast leiomyoma and atypical leiomyoma
Authors: Long Mengping , Hu Xuejiao Lina , Zhao Guiyang , Liu Yiqiang , Hu Taobo 
Published: April 2022

Abstract
Background
Breast leiomyoma is a rare benign mesenchymal tumor, accounting for less than 1% of all breast neoplasms. Cases of breast atypical leiomyoma is even more rarely reported and its diagnostic criteria together with its clinical courses is not cleared defined.

Case presentation
We described two patients with breast leiomyomas. One has unilateral benign breast leiomyoma, the other one has bilateral breast leiomyomas. For the bilateral case, the left-side tumor was diagnosed as benign leiomyoma while the right-side tumor was diagnosed as atypical leiomyoma. The morphological features that lead to the diagnosis of atypical leiomyoma are its invasive growth pattern, mild nuclear atypia, and mitotic figures up to 3mitoses/10HPF.

Conclusions
Atypical breast leiomyoma appears to behave like benign leiomyoma without recurrence in our study with nine-year follow-up. Due to the limited experience, cases presented as atypical intraparenchymal breast leiomyoma should be closely followed.
Facilitators and barriers to implementation of integrated community case management of childhood illness: a qualitative case study of Kapiri Mposhi District
Authors: Riri Johnson Vonje , Silumbwe Adam , Mweemba Chris , Zulu Joseph Mumba 
Published: April 2022

Abstract
Background
Zambia adopted the Integrated Community Case Management (ICCM) of childhood illness strategy in May 2010, targeting populations in rural communities and hard-to-reach areas. However, evidence suggests that ICCM implementation in local health systems has been suboptimal. This study sought to explore facilitators and barriers to implementation of ICCM in the health system in Kapiri Mposhi District, Zambia.

Methods
Data were gathered through 19 key informant interviews with district health managers, ICCM supervisors, health facility managers, and district health co-operating partners. The study was conducted in Kapiri Mposhi district, Zambia. Interviews were translated and transcribed verbatim. Data were were analyzed using thematic analysis in NVivo 11(QSR International).

Results
Facilitators to implementation of ICCM consisted of community involvement and support for the program, active community case detection and timeliness of health services, the program was not considered a significant shift from other community-based health interventions, district leadership and ownership of the program, availability of national and district-level policies supporting ICCM and engagement of district co-operating partners. Program incompatibility with some socio-cultural and religious cotexts, stock-out of prerequisite drugs and supplies, staff reshuffle and redeployment, inadequate supervision of health facilities, and nonpayment of community health worker incentives inhibited implementation of ICCM.

Conclusion
The study findings highlight key faciliators and barriers that should be considered by policy-makers, district health managers, ICCM supervisors, health facility managers, and co-operating partners, in designing context-specific strategies, to ensure successful implementation of ICCM in local health systems.
Adapting community-based sexual and reproductive health services for adolescents and young people aged 15-24 years in response to COVID-19 in Lusaka, Zambia: the implications on the uptake of HIV testing services
Authors: Phiri Mwelwa Muleba , Hensen Bernadette , Schaap Ab , Sigande Lucheka , Simuyaba Melvin , Simwinga Musonda , Floyd Sian , Fidler Sarah , Hayes Richard , Ayles Helen 
Published: April 2022

Abstract
Background
Across Sub-Saharan Africa, adolescents and young people (AYP) aged 15-24 have limited access to sexual and reproductive health (SRH) services, including HIV testing services (HTS). In response, the Yathu Yathu study was implemented in two high-density communities in Lusaka, Zambia. Yathu Yathu provides comprehensive, community-based, peer-led SRH services, including differentiated HTS (finger-prick and HIV self-testing) and comprehensive sexuality education (CSE). We describe adaptations to the Yathu Yathu intervention in response to the COVID-19 epidemic, and implications on uptake of HTS among AYP.

Methods
Yathu Yathu provides SRH services through community-based peer-led spaces. AYP in study communities were offered prevention points cards (PPC), which incentivizes and tracks service use. Social media (WhatsApp©/Facebook©) is used to engage and inform AYP about SRH. Due to COVID-19, hubs closed from April-June 2020. We describe adaptations in response to COVID-19 and, using routinely collected PPC data, describe uptake of HTS before (September 2019-March 2020) and after (July-December 2020) adaptations in response to COVID-19. We describe reach of the Yathu Yathu Facebook page and use qualitative data to describe AYP experiences of SRH service access.

Results
During hub closures, CSE was delivered via video on social media, resulting in an increase in Facebook page followers from 539(April) to 891(June). WhatsApp groups evolved as a platform to deliver CSE and COVID-19 information, with higher participation among young people aged 20-24. Key service delivery adaptations included: reducing the number of participants in hubs, mandatory handwashing before entry, use of personal protective equipment by staff and provision of facemasks to AYP. HTS were provided as normal. Adaptations led to fewer AYP attending hubs. Uptake of HTS among AYP visiting hubs for the first time after COVID-19-related closures was higher (73.2%) compared to uptake before adaptations (65.9%; adjOR=1.24 95%CI 0.99, 1.56, p=0.06). Despite disappointments with some aspects of service delivery, AYP expressed happiness that hubs had reopened.

Conclusions
Social media can be a useful additional platform to reach AYP with HIV prevention information during COVID-19. With proper infection control in place, HTS can safely be provided to, accessed and accepted by AYP in community-based settings during COVID-19.

Trial Registration
National Clinical Trials NCT04060420,19th August 2019. Current Controlled Trials ISRCTN75609016, 14th September 2021, retrospectively registered.
Evaluating the effectiveness of enhanced family planning education on knowledge and use of family planning in fishing communities of Lake Victoria in Uganda: a randomized controlled trial
Authors: Nanvubya Annet , Wanyenze Rhoda K. , Abaasa Andrew , Nakaweesa Teddy , Mpendo Juliet , Kawoozo Barbarah , Matovu Francis , Nabukalu Sarah , Omoding Geoffrey , Kaweesi Jed , Ndugga John , Bagaya Bernard , Chinyenze Kundai , Price Matt A. , Van Geertruyden Jean Pierre 
Published: April 2022

AbstractIntroductionFamily planning knowledge is poor and use is low in Ugandan fishing communities. We compared the effectiveness of enhanced family planning (FP) education with routine counselling on FP knowledge and use.MethodsIndividuals aged 15–49 years were randomly assigned to intervention or control arm. The intervention constituted enhanced FP education based on a simplified handout extracted from the WHO FP guidance tool called, “Family planning: A global handbook for FP providers” which participants took home for additional reading. The control arm constituted FP counselling following Uganda Ministry of Health guidelines. FP knowledge score and contraceptive prevalence rate (CPR) were compared between trial arms at baseline and at 12 months. Negative binomial regression models were used to estimate the effect of the intervention on FP knowledge and use.ResultsOverall, 1410 participants were screened to enrol 1004 (502 per study arm, 48.5% women). Subsequently, 384 (76.5%) and 383 (76.3%) completed the 12 months’ follow-up in the intervention and control arms respectively. At baseline, a median FP knowledge score of 8 and a < 70% FP knowledge score was observed for all participants with a CPR of 36.8%. At month-12, the median FP knowledge score improved in both arms, higher in the intervention arm than the control arm (46 vs 30;p < 0.001). In the intervention arm, 304 (79.2%) had a score of ≥70 compared with 21 (5.5%) in the control arm (p < 0.001). In the negative binomial regression model, the change in FP knowledge score was 47% higher in the intervention arm than in the control arm (score ratio: 1.47, 95%CI: 1. 43-1.51,p < 0.001). The change in CPR was 16% higher in the intervention arm than in the control arm (Prevalence ratio: 1.16, 95%CI: 1.01-1.34,p < 0.040).InterpretationEnhanced FP education using a simplified FP education handout was more effective in increasing FP knowledge and use compared to routine FP counselling for people living in fishing communities. Innovative FP education interventions are recommended for improving FP knowledge and optimizing uptake in remote-rural settings where literacy levels are low.Trial registrationThe study was registered by the Pan African Clinical Trial Registry on 03 July 2021 with a Trial Registration NumberPACTR202107891858045. “Retrospectively registered”.