bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of m6A RNA methylation markers in the hypothalamus of Atlantic salmon
Authors: Ahi Ehsan Pashay , Frapin Morgane , Hukkanen Mikaela , Primmer Craig R. 
Published: September 2022

AbstractMethylation at the N6-position of adenosine, m6A, is the most abundant mRNA modification in eukaryotes. It is a highly conserved universal regulatory mechanism controlling gene expression in a myriad of biological processes. The role of m6A methylation in sexual maturation, however, has remained largely unexplored. While the maturation process is known to be affected by many genetic and environmental factors, the molecular mechanisms causing variation in the timing of maturation are still poorly understood. Hence, investigation of whether a widespread mechanism like m6A methylation could be involved in controlling of the maturation timing is warranted. In Atlantic salmon (Salmo salar), two genes associated with the age at maturity in human,vgll3andsix6, have been shown to play an important role in maturation timing. In this study, we investigated the expression of 16 genes involved in the regulation of m6A RNA methylation in the hypothalamus of Atlantic salmon with different homozygous combinations oflate(L) andearly(E) alleles forvgll3andsix6genes. We found differential expression ofythdf2.2which encodes an m6A modification reader and promotes mRNA degradation. Its expression was higher insix6*LLcompared to other genotypes as well as immature males compared to matures. In addition, we found that the expression levels of genes coding for an eraser,alkbh5, and for a reader,ythdf1, were higher in the hypothalamus of females than in males across all the different genotypes studied. Our results indicate a potential role of the m6A methylation process in sexual maturation of Atlantic salmon, and therefore, provide the first evidence for such regulatory mechanism in the hypothalamus of any vertebrate. Investigation of additional vertebrate species is warranted in order to determine the generality of these findings.
Various tomato cultivars display contrasted morphological and molecular responses to a heat wave
Authors: Bollier N. , Micol-Ponce R. , Dakdaki A. , Maza E. , Zouine M. , Djari A. , Bouzayen M. , Chevalier C. , Delmas F. , Gonzalez N. , Hernould M. 
Published: September 2022

SummaryClimate change is one of the biggest threats that human society currently needs to face. Heat waves associated with global warming negatively affect plant growth and development and will increase in frequency. Tomato is one of the most produced and consumed fruit in the world but remarkable yield losses occur every year due to the sensitivity of many cultivars to heat stress. New insights into how tomato plants are responding to heat waves will contribute to the development of new cultivars with high yields under harsh temperature conditions. In this study, the analysis of microsporogenesis and pollen germination rate of eleven tomato cultivars after exposure to a simulated heat wave revealed differences between genotypes. The transcriptome of floral buds at two developmental stages of five cultivars selected based on their pollen germination tolerance or sensitivity, revealed common and specific molecular responses implemented by tomato cultivars to cope with heat waves. These data provide valuable insights into the underlying molecular adaptation of floral buds to heat stress and will contribute to the development of future climate resilient tomato varieties.
Mitotic tethering enables en masse inheritance of a shattered micronuclear chromosome
Authors: Trivedi Prasad , Steele Christopher D. , Alexandrov Ludmil B. , Cleveland Don W. 
Published: September 2022

SummaryChromothripsis, the shattering and imperfect reassembly of one (or a few) chromosome(s)1, is an ubiquitous2 mutational process generating localized complex chromosomal rearrangements that drive genome evolution in cancer. Chromothripsis can be initiated by missegregation errors in mitosis3,4 or DNA metabolism5-7 that lead to entrapment of chromosomes within micronuclei and their subsequent fragmentation in the next interphase or upon mitotic entry6,8-10. Here, we use inducible degrons to demonstrate that chromothriptically produced pieces of a micronucleated chromosome are tethered together in mitosis by a protein complex consisting of Mediator of DNA damage checkpoint 1 (MDC1), DNA Topoisomerase II Binding Protein 1 (TOPBP1), and Cellular Inhibitor of PP2A (CIP2A), thereby enabling en masse segregation to the same daughter cell. Such tethering is shown to be crucial for the viability of cells undergoing chromosome missegregation and shattering after transient inactivation of the spindle assembly checkpoint. Pan-cancer analysis of transcriptome and whole-genome sequencing data revealed that chromothriptic genome rearrangements were accompanied by elevated expression of MDC1, TOPBP1, and CIP2A. Thus, chromatin-bound tethers maintain proximity of fragments of a shattered chromosome enabling their re-encapsulation into, and religation within, a daughter cell nucleus to form heritable, chromothriptically rearranged chromosomes found in the majority of human cancers.
FRIZZLED 2 regulates limb development by mediating both β-catenin-dependent and independent Wnt signaling pathways
Authors: Zhu Xuming , Xu Mingang , Leu N. Adrian , Morrisey Edward E. , Millar Sarah E. 
Published: September 2022

AbstractHuman Robinow Syndrome and omodysplasia, characterized by skeletal limb and craniofacial defects, are associated with mutations in the Wnt receptor FZD2. However, as FZD2 can activate both canonical and non-canonical Wnt pathways, its precise functions and mechanisms of action in limb development are unclear. To address these questions, we generated mice harboring a single nucleotide insertion in the Dishevelled-interacting domain of Fzd2 (Fzd2em1Smill), causing a frameshift mutation similar to the effects of human syndromic FZD2 mutations. Fzd2em1Smill mutant mice had shortened limbs resembling those of Robinow Syndrome and omodysplasia patients. Fzd2em1Smill mutant embryos displayed decreased canonical Wnt signaling in developing limb mesenchyme and disruption of digit chondrocyte elongation and orientation, which is controlled by the WNT5A/PCP pathway. In line with this, we found that tissue-specific disruption of Fzd2 function in limb mesenchyme caused formation of shortened bone elements and was associated with deficiency in both Wnt/β-catenin and WNT5A/PCP signaling. These findings indicate that FZD2 controls limb development by mediating both canonical and non-canonical Wnt pathways and reveal causality of pathogenic FZD2 mutations in Robinow Syndrome and omodysplasia patients.Summary statementHuman FZD2 mutations are associated with limb defects; using genetic mouse models we revealed causality of these mutations and showed that they disrupt both canonical and non-canonical Wnt signaling.
Contact patterns of UK home delivery drivers and their use of protective measures during the COVID-19 pandemic: a cross-sectional study
Authors: Bridgen Jessica RE , Wei Hua , Whitfield Carl , Han Yang , Hall Ian , Jewell Chris P , van Tongeren Martie JA , Read Jonathan M 
Published: September 2022

ABSTRACTObjectivesTo quantify contact patterns of UK home delivery drivers and identify protective measures adopted during the pandemic.MethodsWe conducted a cross-sectional online survey to measure the interactions of 170 UK delivery drivers during a working shift between 7 December 2020 and 31 March 2021.ResultsDelivery drivers had a mean number of 71.6 (95% Confidence Interval (CI) 61.0 to 84.1) customer contacts per shift and 15.0 (95%CI 11.19 to 19.20) depot contacts per shift. Maintaining physical distancing with customers was more common than at delivery depots. Prolonged contact (more than 5 minutes) with customers was reported by 5.4% of drivers on their last shift. We found 3.0% of drivers had tested positive for SARS-CoV-2 since the start of the pandemic and 16.8% of drivers had self-isolated due to a suspected or confirmed case of COVID-19. Additionally, 5.3% (95%CI 2.3% to 10.2%) of participants reported having worked whilst ill with COVID-19 symptoms, or with a member of their household having a suspected or confirmed case of COVID-19.ConclusionDelivery drivers had a large number of face-to-face customer and depot contacts per shift compared to other working adults during this time. However, transmission risk may be curtailed as contact with customers was of short duration. Most drivers were unable to maintain physical distance with customers and at depots at all times. Usage of protective items such as face masks and hand sanitizer was widespread.
Genome-wide analyses reveal novel opioid use disorder loci and genetic overlap with schizophrenia, bipolar disorder, and major depression
Authors: Holen Børge , Shadrin Alexey A. , Icick Romain , Hindley Guy , Rødevand Linn , O’Connell Kevin S. , Frei Oleksandr , Bahrami Shahram , Cheng Weiqiu , Parker Nadine , Tesfaye Markos , Jahołkowski Piotr , Karadag Naz , Dale Anders M. , Djurovic Srdjan , Smeland Olav B. , Andreassen Ole A. 
Published: September 2022

AbstractOpioid use disorder (OUD) and mental disorders are often comorbid, with increased morbidity and mortality. The causes underlying this relationship are poorly understood. Although these conditions are highly heritable, their shared genetic vulnerabilities remain unaccounted for. We applied the conditional/conjunctional false discovery rate (cond/conjFDR) approach to analyse summary statistics from independent genome wide association studies of OUD, SCZ, BD and MD. Next, we characterized the identified shared loci using biological annotation resources. OUD data was obtained from the Million Veteran Program (15,756 cases 99,039 controls). SCZ (53,386 cases 77,258 controls), BD (41,917 cases 371,549 controls) and MD (170,756 cases 329,443 controls) data was provided by the Psychiatric Genomics Consortium. We discovered genetic enrichment for OUD conditional on associations with SCZ, BD, MD and vice versa, indicating polygenic overlap with identification of 14 novel OUD loci at condFDR<0.05 and 7 unique loci shared between OUD and SCZ (n=2), BD (n=2) and MD (n=7) at conjFDR<0.05 with concordant effect directions, in line with estimated positive genetic correlations. Two loci were novel for OUD, one for BD and one for MD. Three OUD risk loci were shared with more than one psychiatric disorder, at DRD2 on chromosome 11 (BD and MD), at FURIN on chromosome 15 (SCZ, BD and MD), and at the major histocompatibility complex region (SCZ and MD). Our findings provide new insights into the shared genetic architecture between OUD and SCZ, BD, and MD, indicating a complex genetic relationship, suggesting overlapping neurobiological pathways.
Loss of ANT1 Increases Fibrosis and Epithelial Cell Senescence in Idiopathic Pulmonary Fibrosis
Authors: Boatz Jennifer C. , Sui Justin , Hu Qianjiang , Li Xiaoyun , Zhang Yingze , Königshoff Melanie , Kliment Corrine R. 
Published: September 2022

ABSTRACTIdiopathic Pulmonary Fibrosis (IPF) is an interstitial lung disease characterized by progressive lung scarring and remodeling. Although treatments exist that slow disease progression, IPF is irreversible and there is no cure. Cellular senescence, a major hallmark of aging, has been implicated in IPF pathogenesis, and mitochondrial dysfunction is increasingly recognized as a driver of senescence. Adenine nucleotide translocases (ANTs) are abundant mitochondrial ATP-ADP transporters critical for regulating cell fate and maintaining mitochondrial function. We sought to determine how alterations in ANTs influence cellular senescence in pulmonary fibrosis. We found SLC25A4 (ANT1) and SLC25A5 (ANT2) expression is reduced in the lungs of IPF patients and particularly within alveolar type II cells by single cell RNA sequencing. Loss of ANT1 by siRNA in lung epithelial cell lines resulted in increased senescence markers such as beta-galactosidase staining and p21 by Western Blot and RT-qPCR. Bleomycin treated ANT1 knockdown cells also had increased senescence markers when compared to bleomycin treated control cells. Global loss of ANT1 resulted in worse lung fibrosis and increased senescence in the bleomycin and asbestos-induced mouse models of pulmonary fibrosis. This data supports the concept that loss of ANT1 drives IPF pathogenesis through mitochondrial dysfunction associated cellular senescence (MiDaS). In summary, loss of ANT1 induces cellular senescence, leading to abnormal tissue remodeling and enhanced lung fibrosis in IPF. Modulation of ANTs presents a new therapeutic avenue that may alter cellular senescence pathways and limit pulmonary fibrosis.
Optimal Covid-19 vaccine stations location and allocation strategies
Authors: Kumar Akhilesh , Kumar Gaurav , Ramane Tanaya Vijay , Singh Gurjot 
Published: September 2022

PurposeThis study proposes strategies for vaccine center allocation for coronavirus disease (COVID) vaccine by determining the number of vaccination stations required for the vaccination drive, location of vaccination station, assignment of demand group to vaccination station, allocation of the scarce medical professional teams to station and number of optimal days a vaccination station to be functional in a week.Design/methodology/approachThe authors propose a mixed-integer nonlinear programming model. However, to handle nonlinearity, the authors devise a heuristic and then propose a two-stage mixed-integer linear programming (MILP) formulation to optimize the allocation of vaccination centers or stations to demand groups in the first stage and the allocation of vaccination centers to cold storage links in the second stage. The first stage optimizes the cost and average distance traveled by people to reach the vaccination center, whereas the second stage optimizes the vaccine’s holding and storage and transportation cost by efficiently allocating cold storage links to the centers.Findings The model is studied for the real-world case of Chandigarh, India. The results obtained validate that the proposed approach can immensely help government agencies and policymaking body for a successful vaccination drive. The model tries to find a tradeoff between loss due to underutilized medical teams and the distance traveled by a demand group to get the vaccination.Originality/value To the best of our knowledge, there are hardly any studies on a vaccination program at such a scale due to sudden outbreaks such as Covid-19.
A bibliometric analysis of obesity in marketing research
Authors: Onjewu Adah-Kole , Sadraei Razieh , Jafari-Sadeghi Vahid 
Published: September 2022

PurposeIn spite of wide civic and academic interest in obesity, there are no bibliometric records of this issue in the marketing corpus. Thus, this inquiry is conceived to address this shortcoming with a bibliometric analysis of Scopus indexed articles published on the subject.Design/methodology/approachThe analysis followed a five-step science mapping approach of study design, data collection, data analysis, data visualisation and data interpretation. R programming software was used to review 88 peer reviewed journals published between 1987 and 2021.FindingsA sizable stream of literature exploring obesity has accrued in the marketing area as authors have drawn parallels between the influence of persuasive communication and advertising on human wellbeing and child health. The United States of America is found to be by far the country with the highest number of publications on obesity, followed by Australia and the United Kingdom. The topic dendrogram indicates two strands of obesity discourse: (1) social and policy intervention opportunities and (2) the effects on social groups in the population.Research limitations/implicationsThis review will shape future enquiries investigating obesity. Beyond the focus on children, males and females, an emerging focus on cola, ethics, food waste, milk, policy-making and students is highlighted.Originality/valueThis is the first bibliometric review of obesity in the marketing literature. This is especially timely for weighing up the utility of research aimed at understanding and reporting the trends, influences and role of stakeholders in addressing obesity.
Walking on a thin line! Empirical examination of work–home boundary violations faced by employees during forced work from home
Authors: Ramya S.M. , Banu Jasmine , Asokan Ajitha Aswathy , Baral Rupashree 
Published: September 2022

PurposeThis research aims to study employees' pandemic-induced work–home boundary violations using the work–home boundary model. Boundary theory and social theories provide the theoretical underpinnings for this study. The authors study the role of gender, gender role ideology, and fear of COVID-19 in explaining the relationship between work–home boundary violations, work–family conflict (WFC), and subjective well-being (SWB) among working professionals.Design/methodology/approachData were gathered using an online survey on married and working individuals (N = 354) and analyzed using the multi-group analysis technique in structural equation modeling (SEM).FindingsResults show that men faced higher WFC due to job insecurity, while women reported higher WFC due to traditional gender role ideology. Surprisingly, men reported lower subjective well-being due to WFC compared to women, when fear of COVID-19 was low. One promising finding is the potential in using problem-focused coping strategy (PCS) as a boundary-work tactic for both men and women to ensure boundary control (BC) to reduce WFC and improve SWB during the new normal.Practical implicationsThis study contributes to boundary theory, social role theory, and social support resource theory, along with practical implications for employees, organizations, and policymakers.Originality/valueThis study dissects the primary role of problem-focused coping as a valid coping mechanism for managing the issues arising from the pandemic-induced unfavorable working conditions.
Does practice make perfect? Assessing the formation of expertise amongst new venture founders
Authors: Nogueira Tadeu F. , Clausen Tommy H. , Corbett Andrew C. 
Published: September 2022

PurposePrior research has theorised that entrepreneurs use deliberate practice (DP) in the start-up process to improve their competences and achieve new venture success. However, does DP truly lead to an increase in entrepreneurial expertise? This article advances the understanding of DP for entrepreneurship scholars by answering the following question: to what extent does DP influence the formation of entrepreneurial expertise amongst business founders?Design/methodology/approachThe study employs a sample of founders of limited-liability firms. Data were collected mainly through a web-based survey designed specifically for this research. Regression analysis was used to investigate the relationship between DP and entrepreneurial expertise amongst business founders.FindingsThe results show that DP is positively associated with entrepreneurial expertise, which provides us with an improved understanding of DP and expertise in the entrepreneurship context.Originality/valueThe article offers empirical evidence linking DP to the achievement of entrepreneurial expertise. Further, the article emphasises DP as key to experiential learning, representing a predominant mode by which entrepreneurs’ experiences are transformed into expertise. Finally, the article highlights the important role of learning through DP in opportunity development.
The effectiveness of improvisational behavior on sales performance during the COVID-19 pandemic: the moderating effect of functional customer orientation
Authors: Charoensukmongkol Peerayuth , Pandey Arti 
Published: September 2022

Purpose
This research aims to apply the contingency approach to explore the effectiveness of the improvisational behavior of salespeople on their sales performance by considering the moderating role of functional customer orientation.

Design/methodology/approach
Survey data were obtained from a sample of 227 salespeople in Bangkok, Thailand. The data analysis was implemented by partial least squares structural equation modeling.

Findings
The overall results indicate that the association between improvisational behavior and sales performance is significantly contingent on the level of functional customer orientation that salespeople exhibit. For salespeople with high functional customer orientation, improvisational behavior clearly shows a positive association with sales performance. In contrast, for salespeople with low functional customer orientation, improvisational behavior appears to have a negative association with sales performance.

Originality/value
This research broadens the knowledge in the literature by showing that improvisational behavior of salespeople needs to be combined with functional customer orientation to yield a benefit to their sales performance.
The determinants of Tunisian influencer-mompreneurs’ success: an exploratory study of a new form of female web entrepreneurship on Instagram
Authors: Boufares Tayaa Siwar , Bouzaabia Rym 
Published: September 2022

Purpose
This paper is designed with the aim to enlarge our understanding of a new form of women’s entrepreneurship in an emerging market, which is Tunisia. By exploring the role of nowadays’ “influencer-mompreneurs” on Instagram, our research provides a springboard to holistically investigate an up-to-date facet of female entrepreneurship in the digital era and to identify the determinants of its success.

Design/methodology/approach
This study took place in Tunisia and examined a sample of 20 female followers of three successful influencer-mompreneurs. A qualitative exploratory study was conducted through semistructured interviews. To analyze the collected data, the authors used thematic analysis, through which the determinants of influencer-mompreneurs’ success on Instagram bearing on Tunisian followers’ perspectives and previous literature were identified.

Findings
The results suggest that the key determinants of influencer-mompreneurs’ success from their followers’ perspectives depend on their personal traits and their shared content. This is consistent with previous theories and models. In particular, source credibility theory and Ducoffe’s model of advertisement value, which to our knowledge, has not yet been wholly served with regard to influencer marketing research. Paradoxically, the findings reveal that “sharenting” details of their private lives as well as those of their children is also a determinant of influencer-mompreneurs’ success.

Originality/value
Research into influencer-mompreneurs’ success with presence of their children on Instagram can help to provide valuable insights because existing literature into the subject is ambiguous and requires further research to clarify this research gap. Furthermore, by broadening the view of a new form of women entrepreneurship in relation to mother-influencer marketing, this study will inspire future research to dig deeper into creative women owned businesses and to support such emerging economies in the African context.