bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Sr2+, BO33−, and SiO32− on differentiation of human dental pulp stem cells into odontoblast-like cells
Authors: Miyano Yuko , Mikami Masato , Katsuragi Hiroaki , Shinkai Koichi 
Published: November 2022

Abstract
Objectives: This study aimed to clarify the effects of strontium (Sr2⁺), borate (BO33−), and silicate (SiO32−) on cell proliferative capacity, the induction of differentiation into odontoblast-like cells (OLCs), and substrate formation of human dental pulp stem cells (hDPSCs).
Methods: Sr2+, BO33−, and SiO32− solutions were added to the hDPSC culture medium at three different concentrations, totaling nine experimental groups. The effects of these ions on hDPSC proliferation, calcification, and collagen formation after 14, 21, and 28 days of culture were evaluated using a cell proliferation assay, a quantitative alkaline phosphatase (ALP) activity assay, and Alizarin red S and Sirius red staining, respectively. Further, the effects of these ions on hDPSC differentiation into OLCs were assessed via real-time polymerase chain reaction and immunohistochemistry.
Results: Sr2+ and SiO32− increased the expression of odontoblast markers; i.e., nestin, DMP-1, dentin sialophospholipoprotein, and ALP genes, compared with the control group. BO33− increased the ALP gene expression and activity.
Significance: The results of this study suggested that Sr2+, BO33−, and SiO₃²− may induce hDPSC differentiation into OLCs.
Assay for interferon gamma release as a novel marker in pediatric patients with systemic lupus erythematosus
Authors: Zhang Song , Li Xue , Tian Yang , Chen Huishan , Liang Defeng , Chen Kaizhou , Cai Zhe , Zeng Huasong 
Published: November 2022

Abstract
Background: The interferon-gamma (IFN-γ) release assay (IGRA) is an important laboratory diagnosis for latent Mycobacterium tuberculosis (TB) infection. The TB-IGRA measures the release of IFN-γ from peripheral blood cells, who are exposed to TB antigen (Ag), mitogen (MT), or negative/nil control (NL) in vitro. While, an exceptional higher TB Ag-NL level will reflect an elevation of peripheral lymphocytes released IFN-γ in a same condition. Therefore, we found that the elevated levels of TB Ag-NL could become a new biomarker for the diagnosis and treatment of pediatric systemic lupus erythematosus (SLE) patients.
Methods: We have analyzed the clinical data of 776 children who are underwent TB-IGRA testing in the Department of Allergy and Rheumatology of Guangzhou Women and Children's Medical Center from 2018 to 2020. To investigate the association between TB Ag-NL and SLE, we have analyzed the clinical data of 47 SLE patients and TB Ag-NL testing results, and then evaluated the association between TB Ag-NL and SLE disease activity.
Results: The TB Ag-NL levels were significantly higher in patients with active SLE than those in inactive SLE (p=0.0002). The TB Ag-NL levels were positively correlated with the SLE disease activity index (SLEDAI) and laboratory diagnosis parameters. The mean value of TB Ag-NL in SLE patients (0.04191 ± 0.07955, IU/mL) were significantly higher than those in patients with juvenile dermatomyositis (JDM) (0.0158 ± 0.0337, IU/mL, p=0.036), juvenile idiopathic arthritis (JIA) (0.0162 ± 0.0388, IU/mL, p=0.001), and healthy controls (HC) (0.0001 ± 0.0027, IU/mL, p=0.0003). Therefore, the elevated TB Ag-NL levels could serve as a potential diagnostic biomarker of SLE, especially for the active SLE.
Conclusion: The detection of IFN-γ release levels by the TB-IGRA may be useful to assess SLE disease activity in pediatric patients with active SLE.
On the derivation of linear theories for gels
Authors: Barcellos Aline Rigueti , de Souza Angela Cristina Cardoso , Duda Fernando Pereira 
Published: November 2022

Abstract
In this paper we derive a coupled linear theory of elastic deformation and fluid permeation in gels. We start by introducing a nonlinear theory, which describe the state of a gel by its network deformation and interstitial fluid pressure, and then proceed with its linearization about an arbitrary and time-independent state. The linear theory so obtained accounts for the presence of residual stress and involves the tensors of elasticity and permeability which appear from the linearization of the responses for Piola stress and volumetric fluid flow. We present general expressions for these tensors in the case of isotropic gels and particularize the one for the elasticity tensor with respect to isotropic and transversely isotropic states. The linearized equations so obtained are applied to conduct a linear stability analysis of a gel layer attached to a rigid substrate to address the issue of swelling-induced wrinkling. The results are specialized for a gel whose elastic and mixing energies are described by the Gent and Flory–Huggins models.
Variation in dietary resilience among Amazonian populations living in a context of dramatic seasonal flooding
Authors: Tregidgo Daniel , Piperata Barbara , de Lima Jéssica Jaine Silva , Inglez Mariana , Valsecchi João 
Published: November 2022

Abstract
Every year, during one the planet’s greatest seasonal flooding events, Central Amazonian rivers inundate farmland and expand aquatic habitats, making fish harder to catch. Understudied is whether this hydrological regime impacts human dietary intakes/sufficiency, and, if so, whether it varies between communities in seasonally flooded (várzea) and non-flooded (terra firme) zones. To address this gap, we collected dietary data on 8,100 meals over the course of the hydrological cycle in six várzea and three terra firme communities, and calculated energy, carbohydrate, protein, and fat intake. Diets were dominated by fish (44%), the main source of protein and fat, and manioc flour (26%), the main source of energy and carbohydrate. Fish consumption fell as water levels rose, resulting in reduced energy and macronutrient intake in várzea communities. Terra firme communities showed greater seasonal resilience, maintaining protein and fat levels largely by increasing the consumption of wildmeat and fatty palm fruits, respectively.
Biodegradable starch sachets reinforced with montmorillonite for packing ZnO nanoparticles: solubility and Zn2+ ions release
Authors: Peres Laiza Gabriela Sanches , Malafatti João Otávio Donizette , Bernardi Bárbara , Mattoso Luiz Henrique Capparelli , Paris Elaine Cristina 
Published: November 2022

Abstract
Agriculture's importance in human lives and the economy has directed studies to improve crop production. An essential challenge for improving fertilizer efficacy is reducing losses due to leaching and increasing the nutrients supplies. In this context, biodegradable sachets stand out as internal packaging instead of direct insertion into the polymer matrix, facilitating the system processing and making it easier to adapt the soil's nutritional quantity. Thus, the present work aimed to increase the zinc oxide (ZnO) solubility by obtaining nanoparticles using top-down and bottom-up approaches and packaging them in montmorillonite (MMT) reinforced starch sachets. The different diameters and forms of the ZnO nanoparticles were evaluated to understand the solubility dependence on these parameters. In this way, the top-down process was performed for the attritor milling method allowing the nanoparticles with about 71 nm average diameter and greater homogeneity than the commercial one (approximately 174 nm). The milled ZnO nanoparticles presented better solubility than those synthesized bottom-up processes and the commercial ones, reaching a 90 to 100% solubility plateau in 48 h. Concerning starch sachets, the 1% MMT (w w− 1) insertion in the polymeric matrix promoted increased water vapor barrier and mechanical properties, improving the tensile strength. In the solubility test for nanoparticulate ZnO packed in sachets, similar behaviors to free ZnO were observed due to the high affinity of the starch matrix with water. Therefore, starch sachet systems with improved properties from the MMT reinforcement insertion showed as an alternative source of Zn2+ ions to minimize losses during application.
Photothermal seleno-polysaccharide polymer nanocomposites mediating mitochondria function to anti-tumor therapy
Authors: Yang Xiaotong , MA DANDAN , Sheng Enze , Tang Yingying , Tang Yuyin , Hu Linyu , Li Su , Zhu Dong 
Published: November 2022

Abstract
Mediating mitochondria function is essentially challenging but actually significance for biomedicine. However, remote non-invasive regulation of mitochondria function in a living system has been seldom demonstrated and further developed for potential therapy. In here, we prepared a seleno-polysaccharide polymer nanocomposite owning photothermal activity to increase anti-tumor therapy capacity. Such a nanocomposite comprises two key constitutions: near infrared polymer amphiphile and seleno-polysaccharide, acting as photothermal nanotransducer and temperature-sensitive reactive oxygen species (ROS) accelerator, respectively. Under near infrared irradiation (NIR), the productivity of intracellular reactive oxygen species (ROS) can be increased by 1.5-fold to efficiently mitochondrial dysfunction, leading to endogenous mitochondrial apoptosis. This design thus enables provide a promising approach to remotely mediate mitochondria-mediated apoptosis for anti-tumor therapy.
The numerical model of the Internal friction angle and Saturation Degree affect the vertical displacement of the ground
Authors: Doan Thy Truc 
Published: November 2022

Abstract
The numerical model of the different vertical displacement and the internal friction angle variations were determined carefully by the Viet Nam Standard. The difference between experimental values and simulation of the PLAXIS 3D software, was shown remarkably as the maximum value was shown in 0.01975 m (z = -36.9m) depth with ϕ0 = 290); whereas the minimum value in 0.002844 m (z = 0m) depth with ϕ0 = 20. The mean value at the center of the Clay layer (from 0.0m to 27.0m) depth was obtained at 0.0577m whereas compared with 0.0156 m at the Sand layer (from 27.0 to 39.6m) depths.
Moreover, water content (W%) and porosity (P%) were calculated particularly at the maximum value of 94.35% (borehole “HK 2”) with 4.8m depth, whereas the minimum value is 18.22% at 39.3m depth (borehole “HK3”); which compared with porosity (P%) of 71.9% (borehole “HK2”) at 4.8m depth, and 40.4% (borehole “HK3”) at 4.8m depth.
On the other hand, Saturation results presented a relatively low mean value of 88.09% and 86.79% at the center of the ground (from 4.3m to 15.3m). The increase of 23% and 75.42% (from 18.3m to 39.3m) as compared with the research of Zapatata, 1999.
The Rectum, Anal Sphincter and Puborectalis Muscle Show Different Contraction Wave Forms During Deferment of Simulated Feces
Authors: Sun Daming , Lo Kar Man , Chen Ssu-Chi , Leung Wing Wa , Wong Cherry , Mak Tony , Ng Simon , Futaba Kaori , Gregersen Hans 
Published: November 2022

Abstract
Introduction.
The contractile interaction of rectum, puborectalis muscle and anal sphincter must be studied to understand evacuation and consequences of deferred defecation.
Methods.
Six subjects were recruited to study contractile waveforms during deferred defecation with Fecobionics. The subjects filled out symptom questionnaires and had the balloon expulsion test and anorectal manometry done for reference. The Fecobionics bag was filled to urge-to-defecate volume in rectum. The subjects were asked to defer defecation for 4hrs. Pressures and bend angle variations were analyzed with Fast Fourier Transform (FFT). Two subjects were considered either constipated or hypersensitive.
Results.
The remaining four normal subjects were 23.8 ± 1.9yrs. Low frequency signals (< 0.06Hz) were found for pressures and bend angle. The waves were largely uncoordinated between recordings, except for rear and bag pressures. The frequency domain analysis showed waves with amplitude around 0.02-0.04Hz. Differences were found between the pressures and the bend angle both for peak 1 (ANOVA F = 9.403, p < 0.001) and peak 2 amplitudes (F = 5.48, p < 0.005). Post hoc tests showed that the front pressure amplitude was bigger than the others (rear and bend, p < 0.05; bag, p < 0.005) for peak 1, and bigger than bag pressure (p < 0.005) and bend angle (p < 0.05) for peak 2. The constipated subject had lower front pressure amplitudes compared to normal subjects. The other parameters had increased amplitudes. The hypersensitive subject also differed from normal subjects.
Conclusions.
The rectum, anal sphincter and puborectalis muscle showed different contraction waves during deferred defecation. The data call for larger studies to better understand the implications on anorectal disorders.
Real Time Efficient Block Level Dual Mode Data Deduplication Towards Mitigating Side Channel Attack in Cloud Storage
Authors: K Jayashree , K.E Narayana 
Published: November 2022

Abstract
The task of data deduplication and privacy preservation in cloud storage is well studied. There exist numerous approaches to enforce such deduplication and user privacy in cloud storage. However, the methods suffer to achieve expected performance in various metrics. To handle this issue, an efficient Real Time Block Level Dual Mode Data Deduplication (RBDD) scheme is presented in this article. The approach not just concentrates on data deduplication but also focuses on restricting the malicious adversaries at the block level. The method first enforces access restriction in block level based on the key metrics as well as access metrics measured in terms of Access Behavioral Measure (ABM). Also, the method maintains Page Matrix to store the schema of different pages which has information about the blocks, keys and encryption schemes. The dual mode approach encrypts the data encrypted by the user by its own system key which has been dynamically updated and modified at different sessions to handle different threats. The deduplication is performed by verifying the data in block level by computing Block Level Relational Measure (BLRM). Overall, the method improves the performance of data deduplication, public auditing and privacy preservation. Also, the method improves the performance in access restriction towards QOS development.
Improvement the Expression and Purification of Loa22: A Lipoprotein with OmpA domain from Pathogenic Leptospira Serovars
Authors: Gharakhani Mehdi , Ghasemi Mohammad Faezi , Khaki Pejvak , Esmaelizad Majid , Tebianian Majid 
Published: November 2022

Abstract
Background
Outer membrane proteins (OMPs) are the main surface antigens of the pathogenic Leptospires. One of the highly conserved outer membrane proteins expressed only by pathogenic Leptospires is Loa22. This study aims is to obtain the optimum conditions for high expression and purification of Loa22 recombinant (rLoa22) protein.
Methods
Based on evidence of phylogenetic studies, complete coding sequence of loa22 gene was optimized based on codon usage chart and sub-cloned into a pET32a (+) expression vector. BL21 (pLysS) was used as expression host for transformation. The recombinant clones were selected on ampicillin plates and subjected to PCR by using pET T7 primers and expression conditions optimized then by adjusting parameters such as culture media, induction time, temperature, and IPTG concentration.
Results
SDS-PAGE Analysis showed that the production of rLoa22 protein was at the highest level when post induction incubation, IPTG concentration, and duration of induction were 37ºC, 0.1M and 5h in 2xTY medium respectively. Due to the soluble nature of the protein, the purification of the rLoa22 protein under native conditions using Ni-NTA pull-down was optimum in one hour binding process at 37°C, five times washing process and elution buffer with a pH 7.4 and a 0.3 M imidazole concentration.
Conclusion
Based on the results of this study, optimizing the expression and purification process for over production of rLoa22 protein resulted in the large quantity of pure recombinant antigen that forms the basis for future investigation on the design of rapid diagnostic tests and more effective subunit vaccine candidates for leptospirosis.
Minimizing invasiveness and simplifying the surgical procedure for upper and middle early gastric cancer with near-infrared light and organ traction
Authors: Nagano Shinnosuke , Ushimaru Yuki , Kawabata Ryohei , Nishikawa Kazuhiro 
Published: November 2022

Abstract
Background
Surgeons are often faced with optimal resection extent and reconstructive method problems in laparoscopic gastrectomy for gastric cancer in the upper and middle body of the stomach. Indocyanine green (ICG) marking and Billroth I (B-I) reconstruction was used to solve these problems with the organ retraction technique.
Case Presentation
A 51-year-old man with upper gastrointestinal endoscopy revealed a 0-Ⅱc lesion in the posterior wall of the upper and middle gastric body 4 cm from the esophagogastric junction. CT1bN0M0 (cStage IA) was the preoperative diagnosis. Laparoscopic distal gastrectomy (LDG) and D1 + lymphadenectomy was decided to be performed considering postoperative gastric function preservation. The ICG fluorescence method was used to determine the accurate tumor location since the determination was expected to be difficult to the extent of optimal resection with intraoperative findings. By mobilizing and rotating the stomach, the tumor in the posterior wall was fixed in the lesser curvature, and as large a residual stomach as possible was secured in gastrectomy. Finally, delta anastomosis was performed after increasing gastric and duodenal mobility sufficiently. Operation time was 234 min and intraoperative blood loss was 5 ml. The patient was discharged 10 days postoperatively without complications.
Conclusion
The indication for LDG and B-I reconstruction can be expanded to cases where laparoscopic total gastrectomy or LDG and Roux-en-Y reconstruction has been selected for early-stage gastric cancer in the upper gastric body by combining preoperative ICG markings and gastric rotation method dissection.
Brain injury after cranial radiotherapy combined with immunotherapy for brain metastases in lung cancer: a retrospective study
Authors: Li Jiatong , Li Wanhu , Xu Shuhui , Li Yuying , Lu Shuangqing , Chen Feihu , Yu Jinming , Zhu Hui 
Published: November 2022

Abstract

Background: For patients with brain metastasis (BM) from lung cancer, whether cranial radiotherapy (CRT) combined with immune checkpoint inhibitors (ICIs) will increase the risk of radiation-induced brain injury (RBI) remains inconclusive. This retrospective study was performed with the aim of analyzing the incidence of RBI of CRT combined with ICIs and revealing the risk factors forRBI.
Methods: We retrospectively reviewed the medical records of patients with BM from lung cancer who underwent CRT between January 2019 and December 2020 at Shandong Cancer Hospital and Institute. According to whether systemic treatment was used within 6 months before and after CRT, all patients were divided into theCRT+ICIs group and the CRT+non-ICIs group respectively. The diagnosis of brain radiation-induced necrosis (RN) and white matter lesions (WML) was evaluated by brain enhanced MRI. The Fazekas scale and CTCAE v5.0 were used to grade the injury. The risk factors for RBI were identified using univariate and multivariate analyses.
Results: Overall, 210 BM patients undergoing CRT were included in our study. Within 6 months before and after CRT, 56 patients received ICIs, and 154 patients received other systemic therapeutic drugs, including tyrosine kinase inhibitors (TKIs) and chemotherapy. Seventeen (8.1%) patients developed RN, and 142 (67.6%) patients developed WML. The incidence of RN in theCRT+ICIs group vs. the CRT+non-ICIs group was 14.3% vs. 5.8% (p=0.090). However, if ICIs were used within three months of CRT, the incidence of RN in the CRT+ICIs group was significantly higher than that in the CRT+non-ICIs group (18.5% vs. 5.4%, p=0.045).
Multivariate analysis revealed that the maximum diameter of BM > 3.3 cm (p = 0.023) and the total cumulative radiation dose of metastatic lesions > 75.7 Gy (p = 0.018) were risk factors for RN. Additionally, re-radiotherapy was also a trend factor in the development of RN (OR 3.40; 95% CI 0.99-11.67, p=0.051).
Conclusions: ICIs could increase the risk of RN, especially when used within three months of CRT. The total cumulative radiation dose of metastatic lesions is closely related to the development of RN, and re-radiotherapy is also a trend factor in the development of RN.
Multimodal Imaging of Presumed Dusn Associated with Strongyloides Stercoralis Infection
Authors: Urbano Adela , Giralt Lena , Artaraz Joseba , Fonollosa Alex 
Published: November 2022

Abstract
PURPOSE
To report multimodal imaging findings in a case of presumed Diffuse Unilateral Subacute Neuroretinitis (DUSN) associated with Strongyloides stercoralis infection.
METHODS
Retrospective case report.
RESULTS
A thirty-eight-year-old woman presented with visual loss of one week’s duration in her left eye. She had a medical history of chronic dermatitis and epigastralgia. Fundoscopic examination revealed diffuse subretinal inflammatory lesions. Optical coherence tomography showed a disruption of the ellipsoid layer and focal thickenings of the retinal pigment epithelium in the macular region, as well as cells in the vitreous cavity. Fundus autofluorescence showed granular hyperautofluorescence in the posterior pole. Fluorescein angiography revealed leakage of the optic disk and pinpoint disperse hyperfluorescent lesions. Serology tests detected Strongyloides stercoralis antibodies and blood count showed eosinophilia. Treatment with Ivermectine resolved both the ophthalmologic abnormalities and the eosinophilia.
CONCLUSION
DUSN may be associated with Strongyloides stercoralis infection. Inflammatory lesions, if treated early, may resolve without sequelae.
Use of hydrogeochemistry and environmental isotopes for evaluation of groundwater in Qinlian coal base of China
Authors: Zhao Chongqin , Li Xiangquan , Wang Zhenxing , Hou Xinwei , Ma Jianfei 
Published: November 2022

Abstract
The Jiangcang Basin is an important mining area of the former Qilian Mountain large coal base in Qinghai Province. In this study, hydrogeochemical specifications and multiple isotopic tracers are used to propose the main controlling factors of groundwater evolution and groundwater circulation mechanism in the Jiangcang Basin. According to different ion ratios to reveal the main hydrogeochemical processes of groundwater in the study area. The δ18O and the δD isotopes distribution show that suprapermafrost water is recharged by modern meteoric water, while infrapermafrost water is recharged by modern meteoric at the time of nuclear explosion, and the groundwater is closely hydraulically connected to the surface water with weak evaporation overall. Based on the δ34S isotopic characteristics, it is concluded that the high δ34S value of deep groundwater is due to SO4 reduction, and SO42−-rich snow recharge with lixiviated sulfate minerals are the main controlling factor for the high SO42− concentration in groundwater. In addition, using the 14C and the 3H isotopes to date groundwater, we find that the formation age of suprapermafrost water is between 7 and 21 years, that of infrapermafrost water is more than 46 years, that of hot springs is between 8305 and 39600 years, while the formation age of infrapermafrost deep water in the tectonic talik is between 2537 and 6889 years, which has faster updating capacity than thermal springs. According to the multivariate water conversion relationships, it reveals that the river receives more groundwater recharge, suprapermafrost water is recharged by the proportion of meteoric water, which is closely related to the mountainous area at the edge of the basin, while infrapermafrost water is mainly recharged by the shallow groundwater. Through detailed hydrogeological investigation and hydrogeochemical isotope characterization, the groundwater circulation model of the Jiangcang Basin is constructed. The research results are of great significance to the sustainable utilization of groundwater and ecological environmental protection in the coal base of the Tibetan Plateau.