bluuebunny/crossref_metadata_2025_split huggingface dataset:
Manipulating autophagic degradation in human diseases: from mechanisms to interventions
Authors: Zhang Yiqing , Liu Xiaoxia , Klionsky Daniel J , Lu Boxun , Zhong Qing 
Published: October 2022

AbstractTargeted degradation, having emerged as a powerful and promising strategy in drug discovery in the past two decades, has provided a solution for many once undruggable targets involved in various diseases. While earlier targeted degradation tools, as exemplified by PROteolysis-TArgeting Chimera (PROTAC), focused on harnessing the ubiquitin-proteasome system, novel approaches that aim to utilize autophagy, a potent, lysosome-dependent degradation pathway, have also surfaced recently as promising modalities. In this review, we first introduce the mechanisms that establish selectivity in autophagy, which provides the rationales for autophagy-based targeted degradation; we also provide an overview on the panoply of cellular machinery involved in this process, an arsenal that could be potentially harnessed. On this basis, we propose four strategies for designing autophagy-based targeted degraders, including Tagging Targets, Directly Engaging Targets, Initiating Autophagy at Targets, and Phagophore-Tethering to Targets. We introduce the current frontiers in this field, including AUtophagy-TArgeting Chimera (AUTAC), Targeted Protein Autophagy (TPA), AUTOphagy-TArgeting Chimera (AUTOTAC, not to be confused with AUTAC), AuTophagosome TEthering Compound (ATTEC), and other experimental approaches as case studies for each strategy. Finally, we put forward a workflow for generating autophagy-based degraders and some important questions that may guide and inspire the process.
basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method
Authors: Haines Matthew C , Carling Benedict , Marshall James , Shenshin Vasily A , Baldwin Geoff S , Freemont Paul , Storch Marko 
Published: October 2022

Abstract
Standardized deoxyribonucleic acid (DNA) assembly methods utilizing modular components provide a powerful framework to explore designs and iterate through Design–Build–Test–Learn cycles. Biopart Assembly Standard for Idempotent Cloning (BASIC) DNA assembly uses modular parts and linkers, is highly accurate, easy to automate, free for academic and commercial use and enables hierarchical assemblies through an idempotent format. These features enable applications including pathway engineering, ribosome binding site (RBS) tuning, fusion protein engineering and multiplexed guide ribonucleic acid (RNA) expression. In this work, we present basicsynbio, open-source software encompassing a Web App (https://basicsynbio.web.app/) and Python Package (https://github.com/LondonBiofoundry/basicsynbio), enabling BASIC construct design via simple drag-and-drop operations or programmatically. With basicsynbio, users can access commonly used BASIC parts and linkers while designing new parts and assemblies with exception handling for common errors. Users can export sequence data and create instructions for manual or acoustic liquid-handling platforms. Instruction generation relies on the BasicBuild Open Standard, which is parsed for bespoke workflows and is serializable in JavaScript Object Notation for transfer and storage. We demonstrate basicsynbio, assembling 30 vectors using sequences including modules from the Standard European Vector Architecture (SEVA). The BASIC SEVA vector collection is compatible with BASIC and Golden Gate using BsaI. Vectors contain one of six antibiotic resistance markers and five origins of replication from different compatibility groups. The collection is available via Addgene under an OpenMTA agreement. Furthermore, vector sequences are available from within the basicsynbio application programming interface with other collections of parts and linkers, providing a powerful environment for designing assemblies for bioengineering applications.
Graphical Abstract
Complete Capsular Repair Using a Knotless Barbed Suture With a Mini-direct Lateral Approach for Total Hip Arthroplasty: A Technique Note and Feasibility Study
Authors: Kobayashi Naomi , Yukizawa Yohei , Takagawa Shu , Honda Hideki , Kameda Kensuke , Inaba Yutaka 
Published: October 2022

Introduction:
How the hip joint capsule is processed during total hip arthroplasty (THA) and whether a capsular repair is needed, are important factors determining the success of the procedure. In this surgical technique report, we present a detailed technical discussion of the pearls and pitfalls of complete capsular repair using knotless barbed sutures for a mini-direct lateral approach THA.

Materials and Methods:
A total of 45 consecutive cases that underwent a mini-direct lateral approach for THA with a complete capsular repair were included. A historical control group of 169 cases with partial capsulectomy without repair were also reviewed. This technique is based on a modified mini-incision direct lateral approach. A T-shaped capsulotomy was performed. The margin to be seamed at the femoral side of the capsular ligament must be preserved. A knotless barbed suture was applied for repairing the capsular ligament and gluteus minimus.

Results:
In 42 cases (93.3%), a complete capsular repair was achieved successfully. The average operative time was 106±20 minutes. The average intraoperative bleeding was 369±241 mL. There was no major complication both intra and postoperatively.

Conclusion:
Complete capsular repair through a mini-direct lateral approach was feasible by using a knotless barbed suture. Preserving the margin used to seam the femoral side of the capsular ligament is a key requirement for reattachment of the capsule. In addition, a continuous knotless barbed suture facilitates gradual plication of the capsule without a cheese cut.
Patients With High Cardiovascular Risk as Candidates to Bempedoic Acid, After Treatment With Statins, Ezetimibe and PCSK9 Inhibitors: An Estimation and Cost-Effectiveness Analysis
Authors: Seijas-Amigo José , Cordero Alberto , Olmo Rosa F. , Cortez Quiroga Gustavo A. , Fácila Lorenzo , Salgado-Barreira Ángel , Reyes-Santías Francisco , Romero-Menor Cesar , Murillo Juan Rondán , Rodríguez-Mañero Moisés , Bello Mora María C. , Valle Alfonso , Sandin Miriam , Pamias Roman Freixa , Bañeras Jordi , García Pedro Blanch , Lorenzo Milagros Clemente , Sánchez-Alvarez Sergio , López-Rodríguez Luis , González-Juanatey José R. 
Published: October 2022

Abstract:
Low-density lipoprotein cholesterol (LDLc) is the lead effector of atherosclerosis and main treatment target. Bempedoic acid is a novel oral drug in the therapeutic armamentarium which is able to reduce LDLc. The objectives of this study were (1) to select the potential patients for administering bempedoic acid such as those with a very high cardiovascular risk in which objectives of LDLc were not achieved despite conventional treatment with PCSK9 inhibitors (PCSK9i) and/or statins and ezetimibe and (2) to estimate the cost-effectiveness of bempedoic acid in different scenarios. The methods used were a multicenter and retrospective study of 652 patients initiating treatment with any PCSK9 inhibitor in 17 different hospitals. Before and on-treatment LDLc cholesterol levels, medical treatments, clinical indication, and baseline characteristics were recorded. The results obtained from 443 subjects in secondary prevention were analyzed. The mean (±) LDLc level at baseline was 142.5 ± 46.4 mg/dL and 61.5 ± 40.5 mg/dL in the follow-up, with a reduction of 55.9% (P < 0.0001); 71.6% of the patients reached the target of LDL < 55 mg/dL or >50% reduction. Of those patients treated with medium-intensity and low-intensity statins plus PCSK9 inhibitors (with or without ezetimibe), only 5.7% of them were able to reduce LDL below 55 mg/dL and the main LDLc reduction in this group was the lowest (42.9% on average). Patients with TG values >135 mg/dL represented 41.6% of the sample, of which approximately 10% of them were using fibrates. Assuming only LDLc reduction and the UK price, the incremental cost-effectiveness ratio was 88,359€; 83,117€; 82,378€; and 79,015€ for different discount rates. In conclusion, one-third of the patients could achieve the target LDL proposed in the 2019 ESC/EAS guidelines. Approximately 10% of them could also benefit from treating hypertriglyceridemia as indicated in the 2021 ESC guidelines on cardiovascular disease prevention. Patients with medium-intensity and low-intensity statins plus PCSK9i and ezetimibe would be the most benefited. Bempedoic acid could be a not cost-efficacy therapy in all the scenarios, but we need to wait for the CLEAR OUTCOMES Trial results.
Interactions between nutrient intake and comorbidities for quality of life in premenopausal and postmenopausal women
Authors: Nguyen Hai Duc 
Published: October 2022

Abstract

Objective
This study aimed to identify the associations between nutrient intake and health-related quality of life (HRQOL) in premenopausal and postmenopausal women.

Methods
Using data from Korean National Health and Nutrition Examination Surveys (2019‐2020), EQ-5D-3L, and multivariate logistic and linear regression models, we analyzed the association and interaction between nutrient intake, comorbidities, and HRQOL among 4,027 Korean women.

Results
Nutrient intake levels were lower in postmenopausal women than in premenopausal women. Interactions were observed between numerous nutrient intakes and comorbidities for HRQOL among premenopausal and postmenopausal women. Higher intakes of various nutrients (particularly, folic acid and vitamin A) were related to higher HRQOL scores in premenopausal women with comorbidities (log of inverse HRQOL scores, β = −0.31 [95% confidence interval [CI], −0.58 to −0.05] and β = −0.19 [95% CI, −0.37 to −0.001]) and postmenopausal women with comorbidities (log of inverse HRQOL scores, β = −0.34 [95% CI, −0.61 to −0.07] and β = −0.19 [95% CI, −0.38 to −0.01]), respectively. Regular exercise was associated with a lower risk of mobility problems (odds ratio [OR], 0.59; 95% CI, 0.44‐0.81), self-care problems (OR, 0.53; 95% CI, 0.28‐0.99), usual activity problems (OR, 0.42; 95% CI, 0.26‐0.67), and any problem (OR, 0.71; 95% CI, 0.56‐0.90), as well as an increase in HRQOL score (log of inverse HRQOL scores, β = −0.42; 95% CI, −0.68 to −0.16) in postmenopausal women. Smoking was related to a higher prevalence of depression and anxiety problems (OR, 2.51; 95% CI, 1.53‐4.13) and any problem (OR, 1.67; 95% CI, 1.11‐2.52), as well as a decrease in HRQOL scores (log of inverse HRQOL scores, β = 0.51; 95% CI, 0.14‐0.88) in premenopausal women.

Conclusions
Given the lower nutrient intake levels in postmenopausal women, it is critical to establish HRQOL-improving approaches for this population, including regular exercise, nutrient intake, and smoking cessation.
InternalBrace for Intercarpal Ligament Reconstruction
Authors: Starr Brian W. , Ono Shimpei , Seyferth Anne , Chung Kevin C. 
Published: October 2022

SUMMARY:
Intercarpal ligament injuries such as scapholunate and lunotriquetral tears are common causes of wrist pain. There is no shortage of surgical techniques, nor in the volume of literature exploring this topic. However, research progress has not led to a consensus regarding the optimal management of subacute and chronic injuries without articular wear. The senior author (KCC) has performed the spectrum of reconstructive techniques, including dorsal and volar approaches, tendon weaves, ligament tenodesis procedures, reduction and association procedures, and bone-ligament-bone techniques.1,2 In the absence of convincing, consistent outcomes, we began investigating a novel all-dorsal reconstructive technique using the Arthrex InternalBrace system. The complexity and unpredictable outcomes associated with traditional ligament tenodesis procedures led us to adopt the all-dorsal InternalBrace technique as our primary reconstructive approach. We have performed over 20 procedures with this technique since 2019. In an average tourniquet time of under 60 minutes, we are able to achieve predictable carpal stabilization, pain relief, and restoration of motion. This is the preferred reconstructive approach of the senior author.
Stripes and loss of color in ball pythons (Python regius) are associated with variants affecting endothelin signaling
Authors: Dao Uyen M. , Lederer Izabella , Tabor Ray L. , Shahid Basmah ,   The BIO306W Consortium, Graves Chiron W. , Seidel Hannah S. 
Published: October 2022

AbstractColor patterns in non-avian reptiles are beautifully diverse, but little is known about the genetics and development of these patterns. Here we investigated color patterning in pet ball pythons (Python regius), which have been bred to show color phenotypes that differ dramatically from the wildtype form. We report that several color phenotypes in pet animals are associated with putative loss-of-function variants in the gene encoding endothelin receptor EDNRB1: (i) frameshift variants inEDNRB1are associated with conversion of the normal mottled color pattern to skin that is almost fully white, (ii) missense variants affecting conserved sites of the EDNRB1 protein are associated with dorsal, longitudinal stripes, and (iii) substitutions atEDNRB1splice donors are associated with subtle changes in patterning compared to wildtype. We propose that these phenotypes are caused by loss of specialized color cells (chromatophores), with loss ranging from severe (fully white) to moderate (dorsal striping) to mild (subtle changes in patterning). Our study is the first to describe variants affecting endothelin signaling in a non-avian reptile and suggests that reductions in endothelin signaling in ball pythons can produce a variety of color phenotypes, depending on the degree of color cell loss.
Assessment of the influence of weave structure, wind speed and garment size on the ventilation of simulated garment
Authors: Sayanjali Fateme , Ezazshahabi Nazanin , Mousazadegan Fatemeh 
Published: October 2022

PurposeThe aim of the present study is to investigate the effect of fabric weave structure on air permeability and its relation with the garment ventilation.Design/methodology/approachFor this purpose, five groups of cotton/polyester shirting fabrics with plain, T2/1, T2/2, T3/1 and T3/3 weave structures were studied. In order to evaluate ventilation, the garment samples were prepared in different sizes, so that the thickness of the air gap formed between the garment and the body simulator varies by zero, 1.5, 1.2 and 2.9 cm. The effect of wind and its speed (1, 2 and 3 m/s) on clothing ventilation has also been evaluated.FindingsThe results indicated that the rise of wind speed and air gap thickness, due to the increased convective heat transfer, would diminish the air gap temperature of clothing and improves its ventilation. In addition, the fabric weave pattern influences the air ability to pass through the fabric, thus affecting the ventilation capability of the garment.Originality/valueGarments made of fabrics with higher structural firmness, such as the plain, not only have lower air permeability, but also has weaker ventilation capability.
A divide quantified—exploring the relationship between ICT infrastructure diffusion and income inequality
Authors: Kocsis David , Xiong Jason 
Published: October 2022

PurposeInformation and communication technology (ICT) has the potential to address and reduce income inequality. However, since 1980, income inequality in the United States has caused concerns for researchers, policymakers and the public. Entrepreneurs and managers can take advantage of information technologies, while those in the middle and the bottom see fewer benefits. Meanwhile, countries such as Iceland are more capable of using ICT infrastructure to reduce income inequality, which contributes to the well-being of its citizens. This research study explores the relationship between infrastructure diffusion and income inequality through Rogers’s diffusion of innovations theory.Design/methodology/approachTo answer the research questions, the author assessed the data through a series of regression analyses using SPSS. The authors used Power BI software to chart the relationships between ICT infrastructure diffusion and income inequality by country and in the United States by state and region.FindingsThe results show diffusion of innovations theory’s tenets do not necessarily hold, because a significant negative relationship exists between infrastructure diffusion and income inequality, especially in countries with emerging economies. In the United States, this relationship significantly differs by region.Originality/valueThis research contributes to research by expanding economic and sociology work to the IS domain, while providing conflicting evidence for diffusion of innovations theory. The research also provides suggestions for practice, such as more focused ICT infrastructure investments and regulations.
Supporting sustainable marketing programs: exploring relationships between cultural values, green attitudes and intent
Authors: Johnston Nick E. , Jai Tun-Min (Catherine) , Phelan Kelly Virginia , Velikova Natalia 
Published: October 2022

Purpose
This study aims to explore antecedents of supporting local food branded by a sustainable state-operated agricultural marketing program (SOAMP). Cultural values and pro-environmental attitudes were examined as well as their effect on purchase and word-of-mouth intent.

Design/methodology/approach
The values, attitudes and behavior model served as the framework for testing the measurement and structural models and mediation effects. A total of 259 individuals participated in the online survey.

Findings
Structural model was a good fit to the data. The values of collectivism and long-term orientation were the strongest predictors of intent to purchase and spread word of mouth about SOAMP branded products. Green consumption attitudes mediate the relationship from collectivism value to intent to support products branded by a sustainability marketing program.

Originality/value
This study is original in that it challenges the notion that local food consumption is primarily driven by pro-green attitudes. To the best of the authors’ knowledge, it is also the first of its kind to approach local food and pro-green consumption from a cultural perspective by using Hofstede’s cultural values scale to explore how individuals’ values affect their support of products branded by a sustainability marketing program.
A case of palisaded neutrophilic and granulomatous dermatitis associated with systemic lupus erythematosus presenting with pustules
Authors: Tajima Aki , Yamazaki Fumikazu , Huynh Trang Nguyen Mai , Ueda‐Hayakawa Ikuko , Kishimoto Izumi , Itsukage Shizu , Kume Noriko , Tanizaki Hideaki 
Published: October 2022

AbstractPalisaded neutrophilic and granulomatous dermatitis (PNGD) is a relatively rare skin disease that is characterized by a reactive granulomatous histopathological pattern and is often associated with systemic autoimmune diseases. We encountered a case of PNGD that presented with pustules, although the prototypical clinical presentation of PNGD is mainly erythema and papules. Here, this rare case of PNGD with pustules is presented and discussed in relation to the relevant literature.
Interspecific and intraspecific variation of tree branch, leaf and stomatal traits in relation to topography in an aseasonal Amazon forest
Authors: Zuleta Daniel , Muller‐Landau Helene C. , Duque Alvaro , Caro Natali , Cardenas Dairon , Castaño Nicolas , León‐Peláez Juan Diego , Feeley Kenneth J. 
Published: October 2022

Abstract

Tropical forest responses to variation in water availability, which are critical for understanding and predicting the effects of climate change, depend on trait variation among trees.

We quantified interspecific and intraspecific variation in 18 branch, leaf and stomatal traits for 19–72 dominant tree species along a local topographic gradient in an aseasonal Amazon terra firme forest, and tested trait relationships with tree size, elevation, and species' topographic associations. We further tested whether correlation and coordination of traits vary among trees, among species and/or among trees within species.

Intraspecific trait variation was substantial and exceeded interspecific variation in 10 of 18 traits. For leaf acquisition traits, intraspecific variation was mainly related to tree topographic elevation, while most of the variation in branch, leaf and stomatal traits was related to tree size. Interspecific variation showed no clear relationships with species' habitat association. Although trait correlations and coordinations were generally maintained among trees and among species, bivariate relationships varied among trees within species, across habitat association classes and across tree size classes.

Our results demonstrate the magnitude and importance of intraspecific trait variation in tropical trees, especially as related to tree size. Furthermore, these results suggest that previous findings relating interspecific variation with topographic association in seasonal forests do not necessarily generalize to aseasonal forests.

Read the free Plain Language Summary for this article on the Journal blog.
Different ways to success: Plant community trajectories over time and a soil moisture gradient in restored wetlands
Authors: Mosanghini Davide , Oriolo Giuseppe , Boscutti Francesco 
Published: October 2022

Abstract

Ecological restoration is one of the most promising strategies to combat historical wetland losses caused by land use changes. Restored areas are ideal sites to study plant succession and changes in ecosystem functions over time. However, little is known about the influence of restoration on plant succession along environmental stress gradients. Knowing the processes and mechanisms driving the succession over time in contrasting abiotic conditions might provide new insight into the ultimate success of an ecological restoration.

Relying on long‐term vegetation monitoring, we studied the community succession of 4 plant communities along a restored waterlogging gradient in North‐East Italy (from high to low soil saturation level): (i) Cladium fens, (ii) low alkaline fens, (iii) Molina wet meadows and (iv) dry meadows. We monitored 23 permanent plots distributed along the gradient, spanning from 1 to 21 years since restoration, and 4 plots as target vegetation (natural habitats). We analysed the changes in plant communities in terms of functional traits, diversity and species composition.

We found that exotic and annual species decreased in mature stages of restoration while leaf dry matter content increased over time. Nutrient indicator value and leaf area showed opposite trends at the extreme points of the gradient. Across the successional stages, species richness decreased in Cladium fens and increased in alkaline fens and meadows. Species composition moved toward target vegetation showing contrasting dynamics between different restored habitats.

Synthesis and applications. During succession waterlogging stress acts as main abiotic filter, triggering contrasting trajectories of plant communities. This filter seems to be stronger at the extreme points of the gradient generating opposite but faster dynamics than at intermediate conditions. Time and waterlogging promoted a continuous selection of species consistent to target vegetation in terms of richness, functional traits and composition. The evidenced trajectories suggest the need to develop habitat‐specific protocols concerning the selection of restoration site and subsequent management decisions, with particular regard to plant communities at intermediate ecological conditions.