bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibody titer 6 months after the third dose of COVID-19 mRNA vaccination
Authors: Kozakai Rikei , Suzuki Susumu , Hoshi Kuniko , Izumi Yoshihiko , Takahashi Shinichiro 
Published: December 2022

Abstract

Objectives
Administration of the third dose of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccine was initiated on December 1, 2021, in Japan. However, data on the long-term effects of this third vaccination remain scarce. Here, we examined the levels of SARS-CoV-2 antibodies in those who received the Pfizer BioNTech (BNT162b2) vaccine, 6 months after the third vaccination.

Methods
Samples from 40 healthy volunteers were used to measure SARS-CoV-2 antibodies with chemiluminescent assays against the receptor-binding domain (RBD) of the virus.

Results
At 445 days after the first dose of BNT162b2, which is 180 days after the third vaccination, the mean anti-RBD IgG level was 159.4 AU/mL (SD 100.1 AU/mL), which was significantly higher than 144 days after the second vaccination, while mean anti-RBD IgM was baseline level (0.4 C.O.I.). The decline in IgG, 180 days after the third vaccination, was 74.1% (SD 16.1%), which was significantly lower than the 88.6% (SD 4.4%) decline observed 144 days after the second vaccination. Furthermore, we revealed that the reduction in IgG from 14 to 180 days after the third vaccination showed a significant inverse correlation with age, and the higher antibody response in younger participants at 14 days after the third vaccination disappeared at longer time points.

Conclusions
The long-term durability of the IgG titer was significantly higher following the third vaccination compared with the second vaccination, and the reduction in IgG titer after the third vaccination inversely correlated with age.
New properties for the Ramanujan R-function
Authors: Cai Chuan-Yu , Chen Lu , Huang Ti-Ren , Chu Yuming 
Published: December 2022

Abstract
In the article, we establish some monotonicity and convexity (concavity) properties for certain combinations of polynomials and the Ramanujan R-function by use of the monotone form of L’Hôpital’s rule and present serval new asymptotically sharp bounds for the Ramanujan R-function that improve some previously known results.
Research of cooperation strategy of government-enterprise digital transformation based on differential game
Authors: Xie Weihong , Zheng Diwen , Luo Jianbin , Wang Zhong , Wang Yongjian 
Published: December 2022

Abstract
To cope with the development of digital economy, it is particularly important to discuss the digital transformation strategy of government-enterprise cooperation. Taking government and enterprise as game subjects, this paper constructs a game model of government subsidy behavior and enterprise digital transformation effort in the process of digital transformation based on differential game theory and studies different game strategies in noncooperative game, Stackelberg game, and cooperative game. The results show that (1) as an effective regulation mechanism, the government subsidy coefficient can significantly improve the optimal effort of the digital transformation of enterprises; (2) the optimal strategy, the optimal benefit, and the total benefit of the digital ecosystem formed by the government and enterprises in the cooperative game are better than those in the non-cooperative game. Pareto optimality is achieved; (3) the results of theoretical derivation are verified by numerical simulation. This study provides a theoretical basis for the digital transformation cooperation between government and enterprises.
Gray correlation analysis of factors influencing compressive strength and durability of nano-SiO2and PVA fiber reinforced geopolymer mortar
Authors: Zhang Peng , Zhang Xuemei , Zhang Yamin , Zheng Yuanxun , Wang Tingya 
Published: December 2022

AbstractTo investigate the mechanical properties and durability of nano-SiO2, polyvinyl alcohol (PVA) fiber-modified fly ash (FA), and metakaolin (MK)-based geopolymer mortar (FMGM), tests of compressive strength, electrical flux, freeze–thaw cycles, and sulfate dry and wet cycles were conducted. Based on the experimental results, combined with Dunn’s gray correlation theory analysis method, a mathematical analysis of the effect sensitivity of the contents of the four mixtures on the mechanical properties and durability of FMGM was carried out. The method of gray correlation analysis can solve the mathematical problem with partial unclear and uncertain information, and the method requires less data and less computation compared with other mathematical analysis method. The results showed that the gray correlation degree between the content of MK and the electric flux value is higher than the that of other comparison sequence and each reference sequence, while the gray correlation degree between the PVA fiber dosage and the loss rate of compressive strength is lower than that of other comparison sequence and each reference sequence. The influence of the four mixture contents on the compressive strength and mass loss rate was in the following decreasing order: MK and FA, PVA fiber, and nano-SiO2. In addition, the influence of the four material mixture contents on the electric flux value and compressive strength loss rate was consistent in the following decreasing order: MK and FA, nano-SiO2, and PVA fiber. Furthermore, the mechanical properties and durability reached the optimum when the PVA fiber content was 0.6% and the dosage of nano-SiO2was 1.0%. The results of this study can provide a new method for the analysis and evaluation of mechanical properties and durability of nano-SiO2and PVA fiber-reinforced FMGM in future.
Polycaprolactone/epoxide-functionalized silica composite microparticles for long-term controlled release of trans-chalcone
Authors: Kaptan Yasemin , Güvenilir Yüksel 
Published: December 2022

Abstract
In this study, controlled release of trans-chalcone was achieved by using a polycaprolactone-based hybrid system as the drug carrier material. Encapsulation efficiency was obtained in the range of 70–75% for various formulations and in vitro release studies, conducted at 37 °C and pH 7.4, revealed slow profile reaching 60% cumulative release. As interpreted from kinetic modelling, drug release was controlled mainly by Fickian diffusion; polymer erosion did not contribute to the TC release. Difference in drug loading efficiencies of the hybrid and neat PCL microparticles was observed such that PCL microparticles had lower loading efficiency than the hybrid microparticles whereas the release profiles were similar. pH of the release medium had affected release profiles; acidic medium enhanced drug release. Characterization of the microparticles were realized by FT-IR, TGA, DSC, SEM and WCA which revealed key properties such as molecular dispersion state and hydrophilicity. With the results obtained, we concluded that our hybrid system has a significant potential for long term release of trans-chalcone.
Study on laser de-weight and dynamic balance technology of rotating machines
Authors: Tan Zhifu , He Lidong , Deng Chunyan , Zhang Yipeng , Jia Xinyun 
Published: December 2022

Abstract
To solve the poor precision and low efficiency of manual grinding de-weight dynamic balance of turbopump rotor, the laser de-weight dynamic balance technology of rotating machines is adopted. Combined with the advantages of laser de-weight, such as, fast processing speed high machining precision and good machined surface quality, the smooth transition of the machined surface was realized by means of hierarchical machining. According to the characteristics of the turbopump rotor structure, the equivalent transformation model for laser de-weight turbopump rotor was built to convert the unbalance into laser processing parameters, which achieve the rapid and accurate de-weight of turbopump rotor. It focuses on solving the repeatedly loading and low accuracy of manual grinding dynamic balance. To verify the effectiveness of the proposed technique, laser de-weight experiments were carried out on two turbopump rotor test pieces. A dynamic balance test bench was set up to measure the rotor residual unbalance at 1800 r/min test speed, which is low dynamic balance. The results show that the machined surface transition was smooth, the residual unbalance was small and the machining efficiency was high. The effect of laser de-weight dynamic balance reached the expectation. It is an universal technology which is not limited to the dynamic balance of turbopump rotor, but also applicable to other rotating machinery..
Using AAP Guidelines for Managing Febrile Infants Without C-Reactive Protein and Procalcitonin
Authors: Nguyen Tran H.P. , Young Beverly R. , Alabaster Amy , Vinson David R. , Mark Dustin G. , Van Winkle Patrick , Sharp Adam L. , Shan Judy , Rauchwerger Adina S. , Greenhow Tara L. , Ballard Dustin W. 
Published: December 2022

BACKGROUND AND OBJECTIVES
In 2021, the American Academy of Pediatrics (AAP) published the Clinical Practice Guideline (CPG) for management of well-appearing, febrile infants 8 to 60 days old. For older infants, the guideline relies on several inflammatory markers, including tests not rapidly available in many settings like C-reactive protein (CRP) and procalcitonin (PCT). This study describes the performance of the AAP CPG for detecting invasive bacterial infections (IBI) without using CRP and PCT.

METHODS
This retrospective cohort study included infants aged 8 to 60 days old presenting to Kaiser Permanente Northern California emergency departments between 2010 and 2019 with temperatures ≥38°C who met AAP CPG inclusion criteria and underwent complete blood counts, blood cultures, and urinalyses. Performance characteristics for detecting IBI were calculated for each age group.

RESULTS
Among 1433 eligible infants, there were 57 (4.0%) bacteremia and 9 (0.6%) bacterial meningitis cases. Using absolute neutrophil count &gt;5200/mm3 and temperature &gt;38.5°C as inflammatory markers, 3 (5%) infants with IBI were misidentified. Sensitivities and specificities for detecting infants with IBIs in each age group were: 8 to 21 days: 100% (95% confidence interval [CI] 83.9%–100%) and 0% (95% CI 0%–1.4%); 22 to 28 days: 88.9% (95% CI 51.8%–99.7%) and 40.4% (95% CI 33.2%– 48.1%); and 29 to 60 days: 93.3% (95% CI 77.9%–99.2%) and 32.1% (95% CI 29.1%– 35.3%). Invasive interventions were recommended for 100% of infants aged 8 to 21 days; 58% to 100% of infants aged 22 to 28 days; and 0% to 69% of infants aged 29 to 60 days.

CONCLUSIONS
When CRP and PCT are not available, the AAP CPG detected IBI in young, febrile infants with high sensitivity but low specificity.
Bases conceptuales para la compensación ambiental bajo el enfoque ecológico
Authors: Bonilla Fabián , Monrós Juan-S. , Sasa Mahmood 
Published: December 2022

Introducción: La generación de impactos negativos producto de proyectos de infraestructura o actividades de desarrollo generan pérdida de biodiversidad y reducción de la calidad de los servicios que el ambiente provee a sociedades humanas. Afortunadamente, muchos de esos impactos pueden ser reducidos, controlados o resarcidos por medio de medidas de mitigación, rehabilitación y compensación, que suelen sustentarse en el marco jurídico ambiental de los países o estados donde son implementados. Esta revisión provee una visión teórica del uso de compensación ambiental como instrumento jurídico/técnico integrado al manejo del entorno natural.
Metodología: Realizamos una revisión de la literatura global sobre el tema mediante los buscadores de referencias GoogleScholar y SciELO empleando varios indicadores en inglés y español.
Resultados: Un total de 244 referencias sobre compensación ambiental fueron encontradas, catalogadas y evaluadas, mostrándose un claro patrón de incremento del número de ellas en la última década. La compensación ambiental constituye un instrumento jurídico/técnico que permite resarcir por pérdidas ambientales y suele ser un componente de los Estudios de Impacto Ambiental regulado por el estado. Varias estrategias metodológicas son empleadas para establecer acciones compensatorias, cada una de ellas sustentada en alguno de cuatro enfoques posibles: económico, ecológico, político y cultural/social. Dentro del enfoque ecológico, se busca la equivalencia ecológica entre los elementos ambientales impactados y la compensación, procurando que no exista pérdida neta. Así, este enfoque tiene la ventaja de posibilitar la incorporación de múltiples criterios para la evaluación del daño y de las acciones de indemnización, por lo que es posible de aplicar en situaciones diversas. A pesar de su uso, la aplicación de medidas compensatorias no está exenta de crítica y hay situaciones en que la singularidad de los elementos impactados imposibilita un resarcimiento efectivo de las pérdidas.
Conclusiones: La compensación tiene gran relevancia actualmente en la gestión ambiental y debe favorecer el derecho universal a un ambiente sano. Sin embargo, su aplicación efectiva requiere claridad en los procedimientos seguidos y una muy estrecha vigilancia prevenir pérdidas o incluso una ganancia neta ambiental.
Effects of a single dose of psilocybin on cytokines, chemokines and leptin in rat serum
Authors: Bove Geoffrey M. , Mokler David J. 
Published: December 2022

AbstractBackground and AimsThe hallucinogenic drug psilocybin is being widely tested in humans for the treatment of psychiatric disorders. Psilocybin and other psychedelics are proposed to work through serotonin 2a (5-HT2a) receptors, which are tightly linked to immune function. The purpose of the present study was to assess the effects of a single dose of psilocybin on a panel of cytokines, chemokines, and peptides in the short term (24 h) and long term (seven days) in female rats.MethodsFemale rats were given a dose of psilocybin (20 mg kg−1, i.p.} or a dose of synthetic interstitial fluid. At 24 h, the control group and one group of rats were anesthetized, and blood was withdrawn by intracardiac puncture. In a third group of rats, blood was withdrawn after seven days. Serum was analyzed by a separate lab (Eve Laboratories, Calgary, Canada) for 27 immunomodulators.ResultsSerum levels of IL-1β, TNF-α, MCP-1, IP-10, G-CSF, IFN-γ, IL-10, IL-13, and leptin were significantly increased compared to controls after 24 h and were increased further after 7 days. Most of the other assays showed this same pattern of increase, although not statistically significant.ConclusionsPsilocybin induces the release of multiple immune factors, consistent with a generalized activation of the immune system, which can persist for at least seven days after a single dose. These findings may relate to the mechanism of action. The implications of these findings require additional research to determine how these finding relate to the clinical effects of psilocybin.
Transforming US Agriculture: Perspective Directions for Development
Authors: Korotkikh Alla 
Published: December 2022

The escalation of global problems in the XX1 century, such as limited natural resources, climate change, environmental protection and food security, stimulates the technological renewal of world agriculture, its transition to high-tech production methods. The US agriculture is not left aside in this transformation. Today this country is one of the world leaders in terms of the implementation of innovative solutions in agriculture.
Information, bio-, nano-, and robotic technologies transfer makes agricultural work more efficient, and replace human labor. Innovative technologies in Precision Farming like artificial intelligence, GPS, drones and fine-grained data on topography, soils and other aspects of farmland allow farmers to target specific areas with fertilizers, pesticides and water, instead of blanket spraying. It results in
Vertical farming offers many benefits that traditional farming cannot. It uses much less land, allows to grow regional or seasonal crops anywhere, year-round in the controlled environment. As a result, consumers (especially those in urban areas typically far from traditional farmlands) can have easier access to fresher, locally grown vegetables, berries, microgreens.
One third of American farmers are using a few or all precision farming technologies. While vertical farming has a small market share yet, but it has huge growth potential as environment control, and crop production technologies are improved and production costs, especially energy costs, are reduced.
La bioética como un pilar de la formación de profesores de Biología
Authors: Castro Moreno Julio Alejandro 
Published: December 2022

Propósito/Contexto. Este artículo presenta una propuesta acerca de cómo incorporar (o robustecer) la educación bioética en la formación inicial de profesores de biología, en el contexto colombiano.
Metodología/Enfoque. Se inicia con la definición de bioética en la que se basará el artículo, para lo cual se acude a la propuesta de “Bioética Global” de Potter, misma que se amplía, incorporando otras dimensiones, más allá de la ética médica y la ética ambiental. La bioética global, asimismo, se complementa con algunos enfoques: esta perspectiva ha de ser práctica, laica, inter y transdisciplinar, y estar en estrecha relación con la educación.
Resultados/Hallazgos. Se mencionan algunas situaciones de orden nacional que implican debates bioéticos y que podrían desempeñar un rol preponderante en la formación de profesores de biología, situaciones que se sugiere abordar con base en unas estrategias de enseñanza particulares.
Discusión/Conclusiones/Contribuciones. Se afianza la idea de por qué es necesario que los profesores tengan una educación bioética, se enumeran las limitaciones de la propuesta planteada en el documento y se enfatiza en las perspectivas y retos que de ella devienen.
Evaluation of chromogenic agar medium. Can it be a suitable alternative to conventional culture system for identification of uropathogens?
Authors: Sachu Arun , Samuel Abel K 
Published: December 2022

Background and Objectives: Urinary tract infections (UTI) account for major proportion of outpatient load and hospital admission globally. In most of theclinical microbiology laboratories MacConkey agar (MAC) and Cystine lactose electro- lyte-deficient (CLED) agar are being used for identification ofuropathogens. The main objective of the present study was to evaluate the usefulness of HiCromeTM UTI by comparing isolation rate and presumptiveidentification of uropathogens against CLED and MAC agar.
Materials and Methods: This study was conducted over a period of three months on 672 non-duplicate midstream and/or catheter-catch urine samples. All samples were inoculated on to HiCromeTM UTI, CLED agar and MacConkey agar.
Results: Among the 672 samples received for culture, 113 (16.8%)showed significant growth. Among the 672 samples, 95 (14.1%) showed growth of a single organism while 18 (2.7%) showed polymicrobial growth. The rate of isolation and presumptive identification of theisolates and polymicrobial growth was found significantly higher on HiCromeTM UTI Agar.
Conclusion: HiCromeTM UTI Agar has the potential to streamlineprocessing of samples for urine culture in a way that will reduce the workload for technicians, reduce turnaround time which in turn will benefit the laboratoryultimately leading to better patient care.
Effects of nisin on the expression of virulence genes of methicillin- resistant/sensitive Staphylococcus aureus
Authors: Ramezani Mahnaz , Rezazadeh Zarandi Ebrahim , Zainodini Nahid , Bahramabadi Reza , Assar Shokrollah 
Published: December 2022

Background and Objectives: Few studies have considered potential benefits of probiotic bacteria and their derivatives on human and animal health. Nisin is an antimicrobial agent that is produced by lactobacilli and served as a preservative in foods. This study aims to investigate whether nisin suppresses ordecreases the genes involved in the pathogenicity of meth- icillin-susceptible and methicillin-resistant Staphylococcus aureus (MSSA and MRSA).
Materials and Methods: MSSA and MRSA strains were cultured at the ¼, ½, and 1 × minimum inhibitory concentration (MIC) of nisin. Next, RNAextraction was performed at the mid-exponential stage of growth, and cDNA was synthesized. The expression of virulence factors was measured by qPCR, and the data were analyzed by the ΔΔCt formula.
Results: Depending on the incubation times and the Lactobacillus species, the MIC of nisin on MRSA and MSSA observed in 800 and 1600 mg/l, respectively.The qPCR assay showed the expression level of the sea, agrA, and spa genes decreased and the level of the sae gene increased at the sub-MIC of nisin, and no antagonism was observed. Concerning MRSA, the maximum downregulation rate was observed in the sea gene (up to 5.9 folds) while in MSSA, the maximumdownregulation rate was noticed in the agrA gene (up to 10 folds).
Conclusion: Due to the high inhibitory effect of the sub-MIC of nisin on the expression of virulence factor genes in MRSA and MSSA, this compound could potentially reduce the virulence of S. aureus.
Molecular detection of HHV-1, HHV-2, HHV-5 and HBV in semen of fertile and infertile men by multiplex PCR method
Authors: Yasaghi Mohammad , Hosseini Seyyede Delafruz , Moradi Abdolvahab , Hassanpour Mina , Tabarraei Alijan 
Published: December 2022

Background and Objectives: A great diversity of factors including viruses such as human herpes virus 1&2 (HHV-1&2), human herpes virus 5 (HHV-5), andhepatitis B virus (HBV) play key roles in sterility and it is worth noting that male infertil- ity accounts for nearly 50% of barrenness, globally. In this regard, weevaluated the prevalence of the aforementioned viruses in semen specimens of two distinct groups of men referred to Novin Infertility Center in Mashhad, Iran.
Materials and Methods: In this cross-sectional study, 300 semen samples were collected from 150 infertile and 150 fertile men. Subsequently, genomic DNAwas extracted before performing multiplex polymerase chain reaction (PCR). Eventually, the results were analyzed via SPSS Statistics V.16.0.
Results: Out of 300 specimens, 183 (61.1%) were positive at least for one of the forenamed viruses; genome detection of HHV-1&2, HHV-5, and HBV were 27%, 18%, 36.66%, and 4%, respectively.
Conclusion: The current study found no correlation between infertility and HBV, HHV-5, and HHV-1&2, which may have to do with factors like sample size,the geographical distribution of the viruses, and the lifestyle (sexual behavior) of the participants. These results emphasize the implementation of such studies on a broader scale to determine the exact factors involved in infertility.
Tourism Development Towards International Competitive Tourism in Promoting The Economy of East Java
Authors: Yustie Renta , Ariska Ricky Angga , Purwitasari Fadilla 
Published: December 2022

The tourism sector is one of the economic sectors that has a characteristic and contributes to the GRDP (Gross Regional Domestic Product) in East Java. East Java has a large tourism potential with a pattern of tourism development that can contribute to increasing East Java's economic growth. The role of the tourism sector in East Java can improve the national economy and contribute to the national GDP (Gross Domestic Product) of Indonesia. This study aims to determine the development of the tourism sector in East Java during 2016 to 2020 using the LQ (Location Quotient) method so as to produce the tourism sector in the basic or non-basic sector category. The data used is secondary data and this research is a quantitative research. The results of the calculation of LQ (Location Quotient) can help develop and build the tourism sector that is in the basic or non-basic category. Keywords: LQ, GDP, Tourism Sector, Development Economic, East Java