bluuebunny/crossref_metadata_2025_split huggingface dataset:
Independent prognostic significance of TP53 mutations in adult acute myeloid leukaemia with complex karyotype
Authors: Xu Nan , Lai Yue‐Yun , Chen Wen‐Min , Jiang Hao , Wang Yu , Wang Xu , Zhao Xiao‐Su , Huang Xiao‐Jun , Jiang Qian , Qin Ya‐Zhen 
Published: May 2022

AbstractIntroductionAdult acute myeloid leukaemia (AML) patients with complex karyotype (CK) generally have unfavourable outcomes. CK commonly co‐exists with characteristic chromosomal and genetic abnormalities such as monosomal karyotype (MK), −17 or 17p‐ [abn(17p)] and TP53 mutations. Their individual prognostic significance needs to be clarified.MethodsSeventy‐three adult CK‐AML patients and eleven adult non‐CK‐AML patients with TP53 mutations (non‐CK/TP53mu) who were diagnosed and received therapy at our institute were enrolled. One hundred and fifty‐seven AML cases retrieved from the cancer genome atlas (TCGA) for validation.ResultsAmong CK‐AML patients, those with TP53 mutations (CK/TP53mu) had significantly lower rates of 1‐course induction complete remission (CR), 2‐year relapse‐free survival (RFS) and 2‐year overall survival (OS) than those without TP53 mutations (CK/TP53wt); whereas, abn(17p) did not have the above impacts; MK was significantly associated with a lower 2‐year OS rate but was not related to the rates of CR and RFS. Multivariate analysis showed that it were TP53 mutations and treating with chemotherapy alone but not MK and abn(17p) that independently predicted the adverse prognosis for RFS and OS in CK‐AML. Furthermore, non‐CK/TP53mu patients showed similar rates of CR, RFS and OS to CK/TP53mu patients. Validation using the TCGA cohort showed that CK/TP53mu patients had a significantly lower 2‐year OS rate than CK/TP53wt patients, whereas abn(17p) and MK did not impact OS; the 2‐year OS rate of patients with CK/TP53wt was similar to that of patients with intermediate‐risk cytogenetics.ConclusionAdult CK‐AML patients have varied risks and TP53 mutations seem to be an independent adverse prognostic factor.
MicroRNA‐mediated metabolic reprogramming of chimeric antigen receptor T cells
Authors: Rad Seyed Mohammad Ali Hosseini , Halpin Joshua Colin , Tawinwung Supannikar , Suppipat Koramit , Hirankarn Nattiya , McLellan Alexander D 
Published: May 2022

AbstractAdvances made in chimeric antigen receptor (CAR) T cell therapy have revolutionized the treatment and management of certain cancers. Currently, B cell malignancies have been among the few cancers to which CAR T cells have shown persistent and resilient anti‐tumor responses. A growing body of evidence suggests that the persistence of CAR T cells within patients following infusion is linked to the mitochondrial fitness of the CAR T cell, which could affect clinical outcomes. Analysis of CAR T cells from patients undergoing successful treatment has shown an increase in mitochondrial mass and fusion events, and a reduction in aerobic metabolism, highlighting the importance of mitochondria in CAR T cell function. Consequently, there has been recent interest and investment in approaches that focus on mitochondrial programming. In this regard, miRNAs are promising agents in mitochondrial reprogramming for several reasons: (1) natural and artificial miRNAs are non‐immunogenic, (2) one miRNA can simultaneously modulate the expression of multiple genes within a pathway, (3) the small size of a sequence required for producing mature miRNA is ideal for use in viral vectors and (4) different precursor miRNAs (pre‐miRNAs) hairpins can be incorporated into a polycistronic miRNA cluster to create a miRNA cocktail. In this perspective, we describe the latest genetic engineering strategies that can be used to achieve the optimal expression of candidate miRNAs alongside a CAR construct. In addition, we include an in silico analysis of rational candidate miRNAs that could promote the mitochondrial fitness of CAR T cells.
Conflicting temporalities and the unsustainability of the Italian model of migrant personal care assistant
Authors: Vianello Francesca Alice 
Published: May 2022

AbstractIn Southern Europe, the migrant‐in‐the‐family model has become a structural component of the elderly care regime. However, home care work is, by its nature, poorly reconcilable with private and family life. In fact, several studies have denounced the limitations on the right to private and family life that these workers suffer. In this article, I use the migrant home care assistants’ experience of conflicting temporalities around the work–life balance as lens through which to show the social unsustainability of the Italian model of home care assistant. This paper is based on broader research conducted between 2018 and 2020 in Padua (Italy) on Moldovan female migrant workers. As part of this research, 30 semi‐structured interviews were collected with Moldovan workers employed – at the time of the interview or in the past – in home care for elderly people, in a live‐in or live‐out regime.
The role of S-layer protein (SlpA) in biofilm-formation of Deinococcus radiodurans
Authors: Shukla Sudhir K. , Manobala Tamilselvam , Rao Toleti Subba 
Published: May 2022

Abstract

Aims
To investigate the molecular basis of biofilm formation in a recombinant lab strain of Deinococcus radiodurans with a plasmid harbouring gfp and kanR that acquired the biofilm-forming ability.

Methods and results
Deinococcus radiodurans R1 is known as a nonbiofilm former bacterium and so far there are no reports on its biofilm-producing capabilities. In this study, we investigated the molecular basis of biofilm formation in a recombinant strain of D. radiodurans using classical biofilm assays, confocal laser scanning microscopy and real-time PCR. Biochemical analysis of D. radiodurans biofilm matrix revealed that it consisted predominantly of protein and carbohydrate complexes with a little amount of extracellular DNA (eDNA). Furthermore, studies showed that D. radiodurans biofilm formation was enhanced in the presence of 25 mM Ca2+, which enhanced the exopolysaccharide and protein content in the biofilm matrix. Enzymatic treatments with proteinase K, alginate lyase and DNase I indicated the involvement of some proteinaceous components to be critical in the biofilm formation. RT-PCR studies showed that increased expression of a surface layer protein SlpA conferred the biofilm ability to D. radiodurans.

Conclusion
Overexpression of SlpA in D. radiodurans conferred the biofilm formation ability to the bacterium, in which a partial role was also played by the recombinant plasmid pKG. It was also shown that the presence of Ca2+ in the growth medium enhanced SlpA production, thus improving biofilm stability and biofilm maturation of D. radiodurans.

Significance and Impact
This study shows how biofilm formation can be augmented in D. radiodurans. The finding has implications for the development of D. radiodurans biofilm-based biotechnological applications.
Shocks and Time: The Development of the European Financial Assistance Regime
Authors: Rehm Moritz 
Published: May 2022

AbstractThis paper analyses the impact of economic crises on the development of European financial assistance. It demonstrates that crisis episodes that have taken place over the last five decades have significantly altered the design of European Union emergency support. This paper illustrates how solutions adopted in formative moments – including the 1973 oil shock, the Eurozone crisis, and the Covid‐19 pandemic – and their long‐term consequences led to the smorgasbord of instruments of the present financial assistance structure. By applying a historical institutionalist approach, combining insights from studies of critical junctures and gradual change, this contribution explains how economic shocks contributed to change in financial assistance, while also highlighting mechanisms that led to the continuation of specific elements of the assistance structure.
The evolution of the additive variance of a trait under stabilizing selection after autopolyploidization
Authors: Clo Josselin 
Published: May 2022

AbstractWhole‐genome duplication is a common mutation in eukaryotes with far‐reaching phenotypic effects. The resulting morphological, physiological and fitness consequences and how they affect the survival probability of polyploid lineages are intensively studied, but little is known about the effect of genome doubling on the evolutionary potential of populations. Historically, it has been argued polyploids should be less able to adapt because gene duplication dilutes the effects of alleles, such that polyploids are less likely to evolve new adaptive gene complexes compared with diploids. In this paper, I investigate the short‐ and long‐term consequences of genome doubling on the additive genetic variance of populations. To do so, I extended the classical models of quantitative traits under stabilizing selection to study the evolution of the additive variance of the trait under study after a shift from diploidy to tetraploidy. I found that, for realistic allele‐dosage effects, polyploidization is associated with an initial decrease in adaptive potential. In the long term, the better masking of recessive deleterious mutations associated with polyploidy compensates for the initial decrease in additive variance. The time for the tetraploid populations to reach or exceed the additive variance of their diploid progenitors is generally lower than 200 generations. These results highlight that polyploidization per se has a negligible negative effect on the adaptive potential of populations in the short term, and a substantial positive effect in the long term.
Integrated electron beam irradiation treatment with hydrogen peroxide aqueous solution to inactivate Salmonella on grape tomatoes
Authors: Omac Basri , Moreira Rosana G. , Castell‐Perez Elena M. 
Published: May 2022

AbstractThis study investigated the efficacy of integrated electron beam (e‐beam) irradiation and hydrogen peroxide (H2O2) aqueous solution treatments to inactivate mixed strains of Salmonella inoculated in grape tomatoes. Whole grape tomatoes were inoculated with a mixed bacterial cocktail composed of five strains of Salmonella enterica corresponding to serotypes Rissen (SAL1449), Montevideo (SAL4599), Saintpaul (476398), and Typhimurium (ATCC 13311 and ATCC 700720), dried in a biological safety cabinet for 2 h at room temperature and then stored at 4°C for 24 h to facilitate bacterial attachment before treatment. Tomatoes in H2O2 (60 mg/L) aqueous solution were irradiated at 0.45, 0.60, 0.75, 1.0, and 1.25 kGy dose. The combined treatment of 1.0 kGy radiation dose and 60 mg/L H2O2 achieved 4‐log reductions in Salmonella. The D10−average value of Salmonella in the present study was 0.25 ± 0.01 kGy. Regarding the effect of the treatment on tomato quality, the combined application of e‐beam up to 1.25 kGy and H2O2 (60 mg/L) did not affect (p > .05) the color and texture of grape tomatoes compared to non‐irradiated samples. Furthermore, the combined treatments of e‐beam and H2O2 aqueous solution reduced the dose uniformity ratio (DUR) by 7%, a relevant finding regarding commercial applications of electron beam accelerators. Exposure of the tomatoes to e‐beam and water wash with added H2O2 is a promising decontamination system for use in tomatoes and other produce processing. Further studies on the optimization of this integrated treatment are needed.Practical ApplicationsElectron beam irradiation of tomatoes immersed in hydrogen peroxide (H2O2) solution reduces the dose required to eliminate pathogens in the fresh produce. The integrated treatment ensures minimal changes to produce quality and increased treatment efficiency in terms of dose uniformity.
Use of ComBase data to develop an artificial neural network model for nonthermal inactivation of Campylobacter jejuni in milk and beef and evaluation of model performance and data completeness using the acceptable prediction zones method
Authors: Boleratz Bethany L. , Oscar Thomas P. 
Published: May 2022

AbstractComBase is a widely used microbial modeling database. ComBase data can be used to develop and validate models and to test novel modeling methods like artificial neural networks (ANN) and acceptable prediction zones (APZ), which have been shown to outperform traditional methods. Here, ComBase data were used to evaluate the ANN and APZ methods for modeling nonthermal inactivation of Campylobacter jejuni in milk and beef as a function of time, temperature (−20, 1, 10, 20, 30, and 40°C), and strain (18177, ATCC 29428). Four ANN were developed using Excel and NeuralTools, and the best‐performing was a general regression neural network (GRNN) whose performance and data completeness were evaluated using the APZ method. Relative variable impacts in the GRNN model were 42.5%, 31.5%, 20.1%, and 5.9% for time, temperature, food, and strain, respectively. Nonthermal inactivation of C. jejuni was faster and greater at ambient than at cold temperatures and in milk than in beef except at 1°C where it was similar. The proportion of residuals in the APZ (pAPZ) ranged from 0.77 to 1 for individual nonthermal inactivation curves. Although the model had acceptable performance (pAPZ ≥0.7), it failed validation because of data gaps like one instead of four replicates per combination of independent variables and no data at −10°C. Thus, these and other data gaps identified need to be filled before the model can be used with confidence to predict behavior of C. jejuni in milk and beef. Nonetheless, results indicated that ANN and APZ methods can be used to model data for nonthermal inactivation of C. jejuni in food.
Endoscopic radial incision and cutting for benign stenosis of the lower gastrointestinal tract: An investigation of novel endoscopic treatment in multicenter trial
Authors: Moroi Rintaro , Shiga Hisashi , Nochioka Kotaro , Chiba Hirofumi , Shimoyama Yusuke , Kuroha Masatake , Tosa Masaki , Kakuta Yoichi , Kayaba Shoichi , Takahashi Seiichi , Kinouchi Yoshitaka , Masamune Atsushi 
Published: May 2022

AbstractBackground and AimThe standard therapies for benign gastrointestinal stenosis are endoscopic balloon dilation or surgery; each have their advantages and disadvantages. In contrast, radial incision and cutting (RIC) is a novel approach for such stenosis. This study aimed to investigate the feasibility, safety, and effectiveness of RIC.MethodsWe enrolled 20 patients with benign stenosis of the lower gastrointestinal tract developed by various causes and conducted RIC. We evaluated the re‐intervention free rate 52 weeks after RIC, technical success rate, adverse events, procedure time, and improvement of symptoms using a visual analog scale.ResultsWe performed 20 sessions of first RIC for 20 lesions and seven sessions of additional RIC due to re‐stenosis. The cumulative re‐intervention‐free survival rate 52 weeks after the first RIC was 55.8%. The technical success rate of the first RIC was 100% (20/20) while that of the additional RIC was 85.7% (6/7). One case developed perforation during the additional RIC and urgent surgery was performed. The additional RIC tended to show worse results in adverse events and procedure time compared with the first RIC. The patients' symptoms including abdominal bloating and dyschezia were significantly improved.ConclusionsAlthough RIC demonstrated a higher technical success rate for lower gastrointestinal stricture and subsequent improvement of patient symptoms, several issues including preventing delayed bleeding, perforation, and the long‐term prognosis should be solved and clarified in further investigations.
A new stoma for an older person—An association with quality of life and physical function: A systematic review
Authors: Neuberger Lydia , Braude Philip , Weeks Kayleigh , Braude Peter , Halliday Ruth , McCarthy Kathryn , Carter Ben 
Published: May 2022

AbstractBackgroundOlder people are more likely to have a stoma postabdominal surgery than younger people. Few studies have examined the effect of a stoma on older people. The aim of this review was to explore the effect of a stoma on functional independence of an older person. We explored secondary outcomes of poststoma formation length of hospital stay, quality of life and factors affecting stroma independence.MethodsAn exploratory systematic review was developed by our multidisciplinary group including an expert patient, colorectal surgeon, stoma nurse, physiotherapist, geriatrician, and methodologist. Four databases were searched including studies with participants 60 years old or older, who had undergone abdominal surgery for any pathology resulting in an abdominal stoma.ResultsWe identified 857 studies, of which we included 25 in the final review incorporating 6972 participants (average age 67.4 years). There was a strong association between presence of stoma and (1) worse physical function (standardized MD = 0.7; 95% CI 0.21–1.19; I2 = 95) and (2) worse quality of life (standardized MD = 1.61; 95% CI 0.5–2.72, I2 = 98). The same effect was seen in fecal ostomy and urinary diversion. Few studies measured stoma independence and only one examined factors affecting this. No studies examined length of stay.ConclusionsStoma have a negative association with the physical function and quality of life of older people. Future studies should focus on identifying modifiable factors that may affect physical function, quality of life, and stoma independence.
Endpoints and viewpoints on spatial neglect
Authors: McIntosh Robert D. , Ishiai Sumio 
Published: May 2022

AbstractIn this issue of the Journal of Neuropsychology, Abe and Ishiai (2022) report an experiment designed to probe the subjective experience of line bisection in neglect. A re‐analysis of their data can also offer insights into how best to characterise neglect performance for this and other tasks. We show that sensitive measures of neglect can be obtained by quantifying the difference in the influence (or ‘weighting’) that each endpoint has on the response. The right endpoint is dramatically more influential than the left in people with neglect performing line bisection and endpoint reproduction tasks. This supports the view that neglect may limit the ability to simultaneously represent two locations, so that the response is determined primarily with respect to the right endpoint. We also discuss Abe and Ishiai's conclusion that bisection responses in neglect are accompanied by the subjective experience of a complete line extending equally to either side of the chosen midpoint.
How organizational cultures shape social cognition for newcomer voices
Authors: Duan Jinyun , Lin Xiaoshuang , Wang Xiaotian , Xu Yue 
Published: May 2022

AbstractDrawing on cue consensus theory and status characteristics theory, we argue that the consensus between newcomer voice (challenging vs. supportive) and organizational culture (individualistic vs. collectivistic) leads to observers' social cognition of warmth or competence. Based on two survey studies and two experiment studies, we found that individualistic organizational culture strengthens the positive relationship between challenging voice and perceived competence and that voice constructiveness mediates this moderating effect. We also found that collectivistic organizational culture strengthens the positive relationship between supportive voice and perceived warmth, and prosocial motivation mediates this moderating effect. Focusing on consistent cues between voice and organizational culture, our research contributes to the voice literature, answering when and how both newcomer challenging voice and supportive voice can lead to perceived warmth and competence. Our results offer both theoretical implications and practical insights for employees and organizations.
“She will control my son”: Navigating womanhood, English and social mobility in India
Authors: Highet Katy 
Published: May 2022

AbstractThrough its colonial, class‐ and caste‐based history, English in India has come to be seen as a powerful resource that opens doors for those who ‘have’ it and holds back those who do not. For women, English ostensibly offers various promises in addition to employment: progressiveness and ‘empowerment’; and the potential for upward mobility through marriage. Yet, the conversion of English capital for English‐speaking Indian women proves to be intensely complex in practice, as many find themselves forced to navigate between shifting moral regimes attached to ‘tradition’ and ‘modernity’. Drawing on ethnographic fieldwork in an NGO in Delhi that offers free English training to ‘disadvantaged youth’, this paper explores how English capital is managed by young women striving to attain middle classness through English, and how their class, caste and gender positionings are negotiated across particular time‐space configurations as they seek to become English speakers.
The dark side of business model innovation: An empirical investigation into the evolvement of customer resistance and the effectiveness of potential countermeasures
Authors: Heidenreich Sven , Freisinger Elena , Landau Christian 
Published: May 2022

AbstractIn the past decade, a core assumption of research on business model innovation (BMI) has been its beneficial character. However, studies have shown that potentially disrupting BMI is not immune to failure. Still, studies that investigate the causes of BMI failures are lacking. This article shifts the focus to the dark side of BMI by using a demand‐side approach, which cross‐fertilizes on the new product development (NPD) research stream of passive innovation resistance. We argue that BMI, like any other type of innovation, imposes change on the customer, which endangers the status quo. As a result, passive innovation resistance evolves, potentially disrupting continuous adoption. Thus, the main goal of the current study is to investigate whether and how BMI evokes negative effects of passive innovation resistance on customers' adoption behavior (Study 1) and to determine which marketing instruments can be used as countermeasures (Study 2). Our findings confirm that passive innovation resistance is a strong inhibitor of continuous BMI adoption. However, the detrimental effects of passive innovation resistance on continuous BMI adoption can be attenuated by employing benefit comparisons or testimonials in business model (BM) announcements. From a theoretical perspective, this study enhances the current knowledge on how stable customer predispositions affect the adoption process of BMI. By so doing, our study confirms the applicability of passive innovation resistance beyond the NPD domain but also sheds light on differences in the cause‐effect mechanism between BMI and product innovation contexts. From a managerial perspective, this study equips managers with effective countermeasures to passive innovation resistance that should reduce the probability of BMI failure.