bluuebunny/crossref_metadata_2025_split huggingface dataset:
The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect
Authors: Wu Yuqiong , Du Jiahui , Wu Qianju , Zheng Ao , Cao Lingyan , Jiang Xinquan 
Published: February 2022

AbstractGinsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system. However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1. Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive. In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly. Then, micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively. It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression. Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1. Significantly, Ginsenoside Rb1 loaded on micro-nano HAp/silk could facilitate osteogenesis and angiogenesis. All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor’s expression of BMSCs. Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.
Effect of PAIP1 on the metastatic potential and prognostic significance in oral squamous cell carcinoma
Authors: Swarup Neeti , Hong Kyoung-Ok , Chawla Kunal , Choi Su-Jung , Shin Ji-Ae , Oh Kyu-Young , Yoon Hye-Jung , Lee Jae-Il , Cho Sung-Dae , Hong Seong-Doo 
Published: February 2022

AbstractPoly Adenylate Binding Protein Interacting protein 1 (PAIP1) plays a critical role in translation initiation and is associated with the several cancer types. However, its function and clinical significance have not yet been described in oral squamous cell carcinoma (OSCC) and its associated features like lymph node metastasis (LNM). Here, we used the data available from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and Clinical Proteomic Tumor Analysis Consortium (CPTAC) to analyze PAIP1 expression in oral cancer. The publicly available data suggests that PAIP1 mRNA and protein levels were increased in OSCC. The high PAIP1 expression was more evident in samples with advanced stage, LNM, and worse pattern of invasion. Moreover, the in vitro experiments revealed that PAIP1 knockdown attenuated colony forming, the aggressiveness of OSCC cell lines, decreasing MMP9 activity and SRC phosphorylation. Importantly, we found a correlation between PAIP1 and pSRC through the analysis of the IHC scores and CPTAC data in patient samples. Our findings suggest that PAIP1 could be an independent prognostic factor in OSCC with LNM and a suitable therapeutic target to improve OSCC patient outcomes.
NICU discharge preparation and transition planning: guidelines and recommendations
Authors: Smith Vincent C. , Love Kristin , Goyer Erika 
Published: February 2022

AbstractIn this section, we present Interdisciplinary Guidelines and Recommendations for Neonatal Intensive Care Unit (NICU) Discharge Preparation and Transition Planning. The foundation for these guidelines and recommendations is based on existing literature, practice, available policy statements, and expert opinions. These guidelines and recommendations are divided into the following sections: Basic Information, Anticipatory Guidance, Family and Home Needs Assessment, Transfer and Coordination of Care, and Other Important Considerations. Each section includes brief introductory comments, followed by the text of the guidelines and recommendations in table format. After each table, there may be further details or descriptions that support a guideline or recommendation. Our goal was to create recommendations that are both general and adaptable while also being specific and actionable. Each NICU’s implementation of this guidance will be dependent on the unique makeup and skills of their team, as well as the availability of local programs and resources. The recommendations based only on expert opinion could be topics for future research.
The proton-activated G protein-coupled receptor GPR4 regulates the development of osteoarthritis via modulating CXCL12/CXCR7 signaling
Authors: Li Rong , Guan Zijing , Bi Shuyan , Wang Fanhua , He Liang , Niu Xin , You Yu , Liu Yuwei , Ding Yi , Siwko Stefan , Wang Ning , Zhang Ziming , Jin Yunyun , Luo Jian 
Published: February 2022

AbstractInflammatory diseases decrease the extracellular environmental pH. However, whether proton-activated G protein-coupled receptors (GPCRs) can regulate the development of osteoarthritis (OA) is largely unknown. In this study, we report that proton-activated GPR4 is essential for OA development. We found a marked increase in expression of the proton-activated GPR4 in human and mouse OA cartilage. Lentivirus-mediated overexpression of GPR4 in mouse joints accelerated the development of OA, including promotion of articular cartilage damage, synovial hyperplasia, and osteophyte formation, while Gpr4 knockout effectively attenuated the development of posttraumatic and aging-associated OA in mice. We also found that inhibition of GPR4 with the antagonist NE52-QQ57 ameliorated OA progression in mice, promoted extracellular matrix (ECM) production, and protected cartilage from degradation in human articular cartilage explants. Moreover, GPR4 overexpression upregulated matrix-degrading enzymes’ expression and inflammation factors under pro-inflammatory and slightly acidic conditions. Mechanistically, GPR4 suppressed chondrocyte differentiation and upregulated cartilage homeostasis through NF-κB/MAPK signaling activation by regulating CXCR7/CXCL12 expression. Together, our results take the lead to illustrate that proton-activated GPCR acts as a key regulator for OA pathogenesis in vivo, and support that GPR4 could be a promising therapeutic target for OA treatment.
Regulation of P53 signaling in breast cancer by the E3 ubiquitin ligase RNF187
Authors: Li Xin , Niu Zhiguo , Sun Chen , Zhuo Shu , Yang Huijie , Yang Xiao , Liu Yun , Yan Cheng , Li Zhongbo , Cao Qi , Ji Guimei , Ding Yinlu , Zhuang Ting , Zhu Jian 
Published: February 2022

AbstractThe tumor suppressor P53 plays critical role in preventing cancer. P53 is rarely mutated and remains functional in luminal-type breast cancer(1). According to current knowledge, wild-type P53 function is tightly controlled by posttranslational modifications, such as ubiquitination. Several ubiquitin ligases have been shown to regulate P53 ubiquitination and protein stability. Here, we report that RNF187, a RING family ubiquitin ligase, facilitates breast cancer growth and inhibits apoptosis by modulating P53 signaling. RNF187 expression was elevated in breast cancer and correlated with breast cancer survival only in the P53 wild-type groups. Bioinformatic analysis showed that the expression of RNF187 was negatively correlated with the expression of P53 target genes, such as IGFBP3 and FAS, in breast cancer. RNF187 depletion inhibited breast cancer growth and facilitated cell death. RNA sequencing analysis indicated that RNF187 could be an important modulator of P53 signaling. Further experiments showed that RNF187 interacts with P53 and promotes its degradation by facilitating its polyubiquitination in breast cancer cells. Interestingly, the in vitro ubiquitin assay showed that RNF187 can directly ubiquitinate P53 in a manner independent of MDM2. These findings reveal a novel direct P53 regulator and a potential therapeutic target for breast cancer.
Clinical and biomarker analyses of sintilimab versus chemotherapy as second-line therapy for advanced or metastatic esophageal squamous cell carcinoma: a randomized, open-label phase 2 study (ORIENT-2)
Authors: Xu Jianming , Li Yi , Fan Qingxia , Shu Yongqian , Yang Lei , Cui Tongjian , Gu Kangsheng , Tao Min , Wang Xiuwen , Cui Chengxu , Xu Nong , Xiao Juxiang , Gao Quanli , Liu Yunpeng , Zhang Tao , Bai Yuxian , Li Wei , Zhang Yiping , Dai Guanghai , Ma Dong , Zhang Jingdong , Bai Chunmei , Huang Yunchao , Liao Wangjun , Wu Lin , Chen Xi , Yang Yan , Wang Junye , Ji Shoujian , Zhou Hui , Wang Yan , Ma Zhuo , Wang Yanqi , Peng Bo , Sun Jiya , Mancao Christoph 
Published: February 2022

AbstractThis randomized, open-label, multi-center phase 2 study (NCT03116152) assessed sintilimab, a PD-1 inhibitor, versus chemotherapy in patients with esophageal squamous cell carcinoma after first-line chemotherapy. The primary endpoint was overall survival (OS), while exploratory endpoint was the association of biomarkers with efficacy. The median OS in the sintilimab group was significantly improved compared with the chemotherapy group (median OS 7.2 vs.6.2 months;P = 0.032; HR = 0.70; 95% CI, 0.50–0.97). Incidence of treatment-related adverse events of grade 3–5 was lower with sintilimab than with chemotherapy (20.2 vs. 39.1%). Patients with high T-cell receptor (TCR) clonality and low molecular tumor burden index (mTBI) showed the longest median OS (15.0 months). Patients with NLR < 3 at 6 weeks post-treatment had a significantly prolonged median OS (16.6 months) compared with NLR ≥ 3. The results demonstrate a significant improvement in OS of sintilimab compared to chemotherapy as second-line treatment for advanced or metastatic ESCC.
Rhodium hydride enabled enantioselective intermolecular C–H silylation to access acyclic stereogenic Si–H
Authors: An Kun , Ma Wenpeng , Liu Li-Chuan , He Tao , Guan Guiyu , Zhang Qing-Wei , He Wei 
Published: February 2022

AbstractThe tremendous success of stereogenic carbon compounds has never ceased to inspire researchers to explore the potentials of stereogenic silicon compounds. Intermolecular C–H silylation thus represents the most versatile and straightforward strategy to construct C–Si bonds, however, its enantioselective variant has been scarcely reported to date. Herein we report a protocol that allows for the enantioselective intermolecular C–H bond silylation, leading to the construction of a wide array of acyclic stereogenic Si–H compounds under simple and mild reaction conditions. Key to the success is (1) a substrate design that prevents the self-reaction of prochiral silane and (2) the employment of a more reactive rhodium hydride ([Rh]-H) catalyst as opposed to the commonly used rhodium chloride ([Rh]-Cl) catalyst. This work unveils opportunities in converting simple arenes into value-added stereogenic silicon compounds.
Partially sintered copper‒ceria as excellent catalyst for the high-temperature reverse water gas shift reaction
Authors: Liu Hao-Xin , Li Shan-Qing , Wang Wei-Wei , Yu Wen-Zhu , Zhang Wu-Jun , Ma Chao , Jia Chun-Jiang 
Published: February 2022

AbstractFor high-temperature catalytic reaction, it is of significant importance and challenge to construct stable active sites in catalysts. Herein, we report the construction of sufficient and stable copper clusters in the copper‒ceria catalyst with high Cu loading (15 wt.%) for the high-temperature reverse water gas shift (RWGS) reaction. Under very harsh working conditions, the ceria nanorods suffered a partial sintering, on which the 2D and 3D copper clusters were formed. This partially sintered catalyst exhibits unmatched activity and excellent durability at high temperature. The interaction between the copper and ceria ensures the copper clusters stably anchored on the surface of ceria. Abundant in situ generated and consumed surface oxygen vacancies form synergistic effect with adjacent copper clusters to promote the reaction process. This work investigates the structure-function relation of the catalyst with sintered and inhomogeneous structure and explores the potential application of the sintered catalyst in C1 chemistry.
Charge-density reduction promotes ribozyme activity in RNA–peptide coacervates via RNA fluidization and magnesium partitioning
Authors: Iglesias-Artola Juan M. , Drobot Björn , Kar Mrityunjoy , Fritsch Anatol W. , Mutschler Hannes , Dora Tang T.-Y. , Kreysing Moritz 
Published: February 2022

AbstractIt has long been proposed that phase-separated compartments can provide a basis for the formation of cellular precursors in prebiotic environments. However, we know very little about the properties of coacervates formed from simple peptides, their compatibility with ribozymes or their functional significance. Here we assess the conditions under which functional ribozymes form coacervates with simple peptides. We find coacervation to be most robust when transitioning from long homopeptides to shorter, more pre-biologically plausible heteropeptides. We mechanistically show that these RNA–peptide coacervates display peptide-dependent material properties and cofactor concentrations. We find that the interspacing of cationic and neutral amino acids increases RNA mobility, and we use isothermal calorimetry to reveal sequence-dependent Mg2+ partitioning, two critical factors that together enable ribozyme activity. Our results establish how peptides of limited length, homogeneity and charge density facilitate the compartmentalization of active ribozymes into non-gelating, magnesium-rich coacervates, a scenario that could be applicable to cellular precursors with peptide-dependent functional phenotypes.
Characterization and functional analysis of the adipose tissue-derived stromal vascular fraction of pediatric patients with osteogenesis imperfecta
Authors: Tauer Josephine T. , Al-Jallad Hadil , Umebayashi Mayumi , Bakhsh Dena , Rauch Damian , Tran Simon D. , Rauch Frank , Hamdy Reggie 
Published: February 2022

AbstractPediatric patients with Osteogenesis Imperfecta (OI), a heritable connective tissue disorder, frequently suffer from long bone deformations. Surgical correction often results in bone non-unions, necessitating revision surgery with autogenous bone grafting using bone-marrow-derived stem cells (BM-SC) to regenerate bone. BM-SC harvest is generally invasive and limited in supply; thus, adipose tissue's stromal vascular fraction (SVF) has been introduced as an alternative stem cell reservoir. To elucidate if OI patients' surgical site dissected adipose tissue could be used as autologous bone graft in future, we investigated whether the underlying genetic condition alters SVF's cell populations and in vitro differentiation capacity. After optimizing SVF isolation, we demonstrate successful isolation of SVF of pediatric OI patients and non-OI controls. The number of viable cells was comparable between OI and controls, with about 450,000 per gram tissue. Age, sex, type of OI, disease-causing collagen mutation, or anatomical site of harvest did not affect cell outcome. Further, SVF-containing cell populations were similar between OI and controls, and all isolated SVF's demonstrated chondrogenic, adipogenic, and osteogenic differentiation capacity in vitro. These results indicate that SVF from pediatric OI patients could be used as a source of stem cells for autologous stem cell therapy in OI.
Behavioural thermal regulation explains pedestrian path choices in hot urban environments
Authors: Melnikov Valentin R. , Christopoulos Georgios I. , Krzhizhanovskaya Valeria V. , Lees Michael H. , Sloot Peter M. A. 
Published: February 2022

AbstractDue to phenomena such as urban heat islands, outdoor thermal comfort of the cities’ residents emerges as a growing concern. A major challenge for mega-cities in changing climate is the design of urban spaces that ensure and promote pedestrian thermal comfort. Understanding pedestrian behavioural adaptation to urban thermal environments is critically important to attain this goal. Current research in pedestrian behaviour lacks controlled experimentation, which limits the quantitative modelling of such complex behaviour. Combining well-controlled experiments with human participants and computational methods inspired by behavioural ecology and decision theory, we examine the effect of sun exposure on route choice in a tropical city. We find that the distance walked in the shade is discounted by a factor of 0.86 compared to the distance walked in the sun, and that shadows cast by buildings have a stronger effect than trees. The discounting effect is mathematically formalised and thus allows quantification of the behaviour that can be used in understanding pedestrian behaviour in changing urban climates. The results highlight the importance of assessment of climate through human responses to it and point the way forward to explore scenarios to mitigate pedestrian heat stress.
Experimental study on the bearing capacity of PZ shape composite dowel shear connectors with elliptical holes
Authors: Huang Pingming , He Junlong , Kong Fanlei , Mei Kuihua , Li Xiaolong 
Published: February 2022

AbstractThe push-out test was carried out in order to study the shear performance of perfobond ribs (PBL) and puzzle (PZ) shape composite dowel shear connectors which are used in corrugated steel–concrete composite bridge decks. By considering the influencing factors of transversal reinforcement, elliptical holes and bonding friction forces, eight specimens were designed and prepared. The load–slip curves were obtained, and the static mechanical properties, including bearing capacity, slip, shear stiffness, and ductility factor, were analyzed. The formula of bearing capacity and load–slip curve were proposed. The results showed a clear deformation of the transversal reinforcement of both the two types of shear connectors. The dominating failure mode of both the shear connectors was the double plane shearing of the concrete dowel. The load–slip curves of the two types of shear connectors followed the same trend. The PZ shape composite dowel performed better in ultimate bearing capacity, ductility, and shear stiffness than the PBL shear connector. Elliptical holes in a PZ shape composite dowel significantly increased the ultimate bearing capacity. The shear stiffness increased with transversal reinforcement and reduced with elliptical holes. For PBL shear connectors, the strain change in the C1 area near the loading point was most apparent. While, the A1 and B1 areas were stress concentration locations for PZ shape composite dowel shear connectors. The two formulas were in good agreement with the test results.
High prevalence of occult left ventricular diastolic dysfunction detected by exercise stress test in systemic sclerosis
Authors: Mohri Takato , Goda Ayumi , Takeuchi Kaori , Kikuchi Hanako , Inami Takumi , Kohno Takashi , Sakata Konomi , Soejima Kyoko , Satoh Toru 
Published: February 2022

AbstractDespite the poor prognosis of systemic sclerosis (SSc) due to the co-occurrence of left ventricular diastolic dysfunction (LVDD), presence of occult LVDD has not been sufficiently investigated. This retrospective study aimed to reveal the prevalence and determinants of occult LVDD in patients with SSc by exercise stress test. Forty-five SSc patients (age, 63 ± 13 years; men/women, 6/39) with normal pulmonary artery pressure and pulmonary artery wedge pressure (PAWP) at rest underwent a symptom-limited exercise test with right heart catheterization using a supine cycle ergometer; haemodynamic parameters at rest, leg raise and during exercise were evaluated. Occult LVDD defined PAWP ≥ 25 mmHg during exercise was seen in 13 patients (29%). Higher PAWP, lower pulmonary vascular resistance and diastolic pulmonary pressure gradient, larger left atrium at rest, and higher PAWP during leg raise (15 ± 4 vs 10 ± 4 mmHg in non-LVDD group, p < 0.001) were observed in the occult LVDD group. The area under the ROC curve for PAWP after leg raise was largest at 0.83 (95% CI: 0.70–0.95, p = 0.001). About one-third (29%) of SSc patients with normal haemodynamics at rest showed occult LVDD. A higher PAWP after leg raise could be useful for detecting occult LVDD.
Detecting disease associated biomarkers by luminescence modulating phages
Authors: Kulpakko Janne , Juusti Vilhelmiina , Rannikko Antti , Hänninen Pekka E. 
Published: February 2022

AbstractAssessment of risk for a given disease and the diagnosis of diseases is often based on assays detecting biomarkers. Antibody-based biomarker-assays for diseases such as prostate cancer are often ambiguous and biomarker proteins are frequently also elevated for reasons that are unspecific. We have opted to use luminescence modulating phages for the analysis of known acute inflammatory response biomarker CRP (C-reactive protein) and biomarkers of prostate cancer in urine samples. Firstly, CRP was used to simulate the detection process in a controlled chemical environment. Secondly, we tried to classify more challenging lethal prostate cancer samples from control samples. Our unique method utilizes a special biopanning process in order to create special phages capable of capturing a dye necessary for detection and potential biomarkers. As the biomarker-molecules interfere with the phages, dye is repelled from the phage network resulting in an altered reporter luminescence. These changes can be observed with an absorbance reader and even with the naked eye. The simple method could present an alternative for screening of disease biomarkers. For prostate cancer urine samples, we achieved a sensitivity of 80% and specificity of 75% to detect Grade Group (GG) 4 and 5 prostate cancer.
Source identification and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in air and dust samples of Lahore City
Authors: Aslam Rabia , Sharif Faiza , Baqar Mujtaba , Shahzad Laila 
Published: February 2022

AbstractDuring two consecutive summer and winter seasons in Lahore, the health risk of air and dust-borne polycyclic aromatic hydrocarbons (PAHs) was evaluated. Gas chromatography/mass spectrometry (GS/MS) was used to determine air and dust samples from various functional areas across the city. The mean ∑16PAHs were higher in air 1035.8 ± 310.7 (pg m−3) and dust 963.4 ± 289.0 (ng g−1 d.w.) during winter seasons as compared to summer seasons in air 1010.9 ± 303.3 (pg m−3) and dust matrices 945.2 ± 283.6 (ng g−1 d.w.), respectively. PAHs ring profile recognized 3 and 4 rings PAHs as most dominant in air and dust samples. Estimated results of incremental lifetime cancer risk (ILCR) highlighted high carcinogenic risk among the residents of Lahore via ingestion and dermal contact on exposure to atmospheric PAHs. The total ILCR values in air among children (summer: 9.61E − 02, winter: 2.09E − 02) and adults (summer: 1.45E − 01, winter: 3.14E − 02) and in dust, children (summer: 9.16E − 03, winter: 8.80E − 03) and adults (summer: 1.38E − 02, winter: 1.33E − 02) during the study period. The isomeric ratios in the study area revealed mixed PAH sources, including vehicular emission, petroleum, diesel and biomass combustion. As a result, it is advised that atmospheric PAHs should be monitored throughout the year and the ecologically friendly fuels be used to prevent PAHs pollution and health concerns in the city. The findings of this study are beneficial to the local regulating bodies in terms of controlling the exposure and promoting steps to reduce PAHs pollution and manage health in Lahore.