bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of total marrow/lymphoid irradiation dose to the intestine on graft-versus-host disease in allogeneic hematopoietic stem cell transplantation for hematologic malignancies
Authors: Saldi Simonetta , Fulcheri Christian Paolo Luca , Zucchetti Claudio , Abdelhamid Amr Mohamed Hamed , Carotti Alessandra , Pierini Antonio , Ruggeri Loredana , Tricarico Sara , Chiodi Marino , Ingrosso Gianluca , Bini Vittorio , Velardi Andrea , Martelli Massimo Fabrizio , Hui Susanta Kumar , Aristei Cynthia 
Published: December 2022

Background and purposeGraft-versus-host disease (GvHD) is a leading cause of non-relapse mortality in patients undergoing allogeneic hematopoietic stem cell transplantation. The Perugia Bone Marrow Transplantation Unit designed a new conditioning regimen with total marrow/lymphoid irradiation (TMLI) and adaptive immunotherapy. The present study investigated the impact of radiotherapy (RT) doses on the intestine on the incidence of acute GvHD (aGvHD) in transplant recipients, analyzing the main dosimetric parameters.Materials and methodsBetween August 2015 and April 2021, 50 patients with hematologic malignancies were enrolled. All patients underwent conditioning with TMLI. Dosimetric parameters (for the whole intestine and its segments) were assessed as risk factors for aGvHD. The RT dose that was received by each intestinal area with aGvHD was extrapolated from the treatment plan for each patient. Doses were compared with those of the whole intestine minus the affected area.ResultsEighteen patients (36%) developed grade ≥2 aGvHD (G2 in 5, G3 in 11, and G4 in 2). Median time to onset was 41 days (range 23–69 days). The skin was involved in 11 patients, the intestine in 16, and the liver in 5. In all 50 TMLI patients, the mean dose to the whole intestine was 7.1 Gy (range 5.07–10.92 Gy). No patient developed chronic GvHD (cGvHD). No dosimetric variable emerged as a significant risk factor for aGvHD. No dosimetric parameter of the intestinal areas with aGvHD was associated with the disease.ConclusionIn our clinical setting and data sample, we have found no clear evidence that current TMLI dosages to the intestine were linked to the development of aGvHD. However, due to some study limitations, this investigation should be considered as a preliminary assessment. Findings need to be confirmed in a larger cohort and in preclinical models.
Immune subtype identification and multi-layer perceptron classifier construction for breast cancer
Authors: Yang Xinbo , Zheng Yuanjie , Xing Xianrong , Sui Xiaodan , Jia Weikuan , Pan Huali 
Published: December 2022

IntroductionBreast cancer is a heterogeneous tumor. Tumor microenvironment (TME) has an important effect on the proliferation, metastasis, treatment, and prognosis of breast cancer.MethodsIn this study, we calculated the relative proportion of tumor infiltrating immune cells (TIICs) in the breast cancer TME, and used the consensus clustering algorithm to cluster the breast cancer subtypes. We also developed a multi-layer perceptron (MLP) classifier based on a deep learning framework to detect breast cancer subtypes, which 70% of the breast cancer research cohort was used for the model training and 30% for validation.ResultsBy performing the K-means clustering algorithm, the research cohort was clustered into two subtypes. The Kaplan-Meier survival estimate analysis showed significant differences in the overall survival (OS) between the two identified subtypes. Estimating the difference in the relative proportion of TIICs showed that the two subtypes had significant differences in multiple immune cells, such as CD8, CD4, and regulatory T cells. Further, the expression level of immune checkpoint molecules (PDL1, CTLA4, LAG3, TIGIT, CD27, IDO1, ICOS) and tumor mutational burden (TMB) also showed significant differences between the two subtypes, indicating the clinical value of the two subtypes. Finally, we identified a 38-gene signature and developed a multilayer perceptron (MLP) classifier that combined multi-gene signature to identify breast cancer subtypes. The results showed that the classifier had an accuracy rate of 93.56% and can be robustly used for the breast cancer subtype diagnosis.ConclusionIdentification of breast cancer subtypes based on the immune signature in the tumor microenvironment can assist clinicians to effectively and accurately assess the progression of breast cancer and formulate different treatment strategies for different subtypes.
Invisible cervical cancers on MRI: Can the type of histology (SCC versus non-SCC) influence surgical planning?
Authors: Jeon Jungeun , Park Byung Kwan , Lee Jeong-Won , Choi Chel Hun , Lee Yoo-Young , Kim Tae-Joong , Kim Byoungi-Gie 
Published: December 2022

BackgroundInvisible cervical cancers on MRI can indicate less invasive surgery. Cervical cancers consist of squamous cell carcinoma (SCC) and non-SCC, each with different long-term outcomes. It is still unclear if surgical planning should be changed according to the histologic type of cervical cancer when it is not visible on MRI.PurposeThe purpose of the study was to determine if surgical planning for cervical cancer that is not visible on MRI is influenced by the histologic type.Materials and methodsBetween January 2007 and December 2016, 155 women had Federation of Gynecology and Obstetrics (FIGO) stage 1B1 cervical cancer that was not visible on preoperative MRI. They underwent radical hysterectomies and pelvic lymph node dissections. Among them, 88 and 67 were histologically diagnosed with SCC and non-SCC, respectively. The size of the residual tumor, depth of stromal invasion, parametrial invasion, vaginal invasion, lymphovascular invasion, and lymph node metastasis were compared between these patients using the t-test, Mann–Whitney U test, Chi-squared test, or Fisher’s exact test. The recurrence-free and overall 10-year survival rates were compared between the groups by Kaplan–Meier analysis.ResultsThe mean sizes of residual tumors were 8.4 ± 10.4 mm in the SCC group and 12.5 ± 11.9 mm in the non-SCC group (p = 0.024). The mean depth of stromal invasion in the SCC group was 12.4 ± 21.2% (0%–100%), whereas that in the non-SCC group was 22.4 ± 24.4 (0%–93%) (p = 0.016). However, there was no difference in parametrial or vaginal invasion, lymphovascular invasion, or lymph node metastasis (p = 0.504–1.000). The recurrence-free and overall 10-year survival rates were 98.9% (87/88) and 95.5% (64/67) (p = 0.246), and 96.6% (85/88) and 95.5% (64/67) (p = 0.872), respectively.ConclusionsThe non-SCC group tends to have larger residual tumors and a greater depth of stromal invasion than the SCC group, even though neither is visible on MRI. Therefore, meticulous care is necessary for performing parametrectomy in patients with non-SCC cervical cancer.
Integrated transcriptome and metabolome analysis reveals the anthocyanin biosynthesis mechanisms in blueberry (Vaccinium corymbosum L.) leaves under different light qualities
Authors: Zhang Jiaying , Li Shuigen , An Haishan , Zhang Xueying , Zhou Boqiang 
Published: December 2022

IntroductionBlueberry (Vaccinium corymbosum L.) is a popular fruit with an abundance of anthocyanins in its leaves and fruits. Light is one of the pivotal environmental elements that affects plant growth and development, but the regulatory mechanism between light quality and anthocyanin formation is poorly understood.MethodsAn integrated transcriptome and metabolome analysis was performed to investigate the effects of white (control), blue (B), red (R), and red/blue (60R/40B) light on blueberry growth and reveal the potential pathway controlling anthocyanin biosynthesis in blueberry leaves.ResultsThe anthocyanin content was significantly improved by the blue and red/blue light when compared with white light, whereas there was a significant reduction in the photosynthesis under the blue light, showing an inverse trend to that of anthocyanin accumulation. Transcriptomic analysis resulted in the assembly of 134,709 unigenes. Of these, 22 were differentially expressed genes (DEGs) that participate in the anthocyanin biosynthesis pathway, with the majority being significantly up-regulated under the blue light. Most of the photosynthesis-related genes that were down-regulated were expressed during anthocyanin accumulation. Targeted metabolome profiling identified 44 metabolites associated with anthocyanin biosynthesis. The contents of most of these metabolites were higher under blue light than the other light conditions, which was consistent with the transcriptome results. The integrated transcriptome and metabolome analysis suggested that, under blue light, leucoanthocyanidin dioxygenase (LDOX), O-methyltransferase (OMT), and UDP-glucose flavonoid glucosyltransferase (UFGT) were the most significantly expressed, and they promoted the synthesis of cyanidin (Cy), malvidin (Mv), and pelargonidin (Pg) anthocyanidins, respectively. The expression levels of dihydroflavonol 4-reductase (DFR) and OMT, as well as the accumulation of delphinidin (Dp), peonidin (Pn), and petunidin (Pt), were significantly increased by the red/blue light.DiscussionThe blue and red/blue lights promoted anthocyanin biosynthesis via inducing the expression of key structural genes and accumulation of metabolites involved in anthocyanin synthesis pathway. Moreover, there was a possible feedback regulating correlation between anthocyanin biosynthesis and photosynthesis under different light qualities in blueberry leaves. This study would provide a theoretical basis for elucidating the underlying regulatory mechanism of anthocyanin biosynthesis of V. corymbosum.
The impact of built environment on physical activity and subjective well-being of urban residents: A study of core cities in the Yangtze River Delta survey
Authors: Zhang Jing , Zheng Yan , Wen Tao , Yang Min , Feng Qiang ming 
Published: December 2022

ObjectiveIn cities with high population density in China, the impact of built environment on human health is rather complicated. Physical activities are an important factor in promoting people’s health. This study is aimed to explore ways of enhancing the residents’ intensity of physical activities and psychological health in a limited built environment. For this purpose, this study conducted research on 1875 residents from cities in the Yangtze River Delta in China to clarify the complicated correlations among the residents’ physical activities, the multi-dimensional geographic environment characteristics, and subjective well-being.MethodsFirst, Neighborhood Environment Walkability Scale (NEWS-A), International Physical Activity Questionnaire Short Form (IPAQ-SF), and Subjective Well-being Scale for Chinese Citizens (SWBS-CC) were used to measure built environment characteristics, intensity of physical activities, and subjective well-being. Second, the correlations among built environment, physical activities, and subjective well-being were analyzed, which reflected different effects of built environment characteristics on physical activities and subjective well-being. Third, physical activities were viewed as a mediating variable in SEM to analyze the influence mechanism of each built environment characteristic on the subjective well-being of residents.ResultResidents with different individual characteristics may have different levels of perception and usage of built environment. The intensity of physical activities has significant positive correlations with proximity to supporting facilities, accessibility of destinations, and public security, while no significant correlation with overall environmental aesthetics and street connectivity. The residents’ subjective well-being has significant positive correlations with accessibility of destinations, overall environmental aesthetics, and public security, while no significant correlation with proximity to supporting facilities and street connectivity. Physical activities not only have a direct effect on subjective well-being, but also a mediating effect on the correlations between subjective well-being and built environment characteristics.ConclusionIn the future, more research could be conducted on the optimization of correlations between residential built environment characteristics and physical activities as well as subjective well-being, so as to gain a deeper understanding about the impact of residential built environment on people’s physical and mental health.
Social support as a mediator of the relationship between forgiveness and post-traumatic growth in hemodialysis patients: A structural equation modeling approach
Authors: Ye Yansheng , Tong Zongwu , Li Changqiong , Gao Xiufang , Sun Yane , Xu Jianqing , Xu Qian , Song Chengrong 
Published: December 2022

BackgroundPost-traumatic growth (PTG) refers to the positive psychological changes experienced with individuals after struggling with highly challenging life circumstances. Forgiveness can facilitate positive outcomes such as reduced distress, anxiety, and depression. Many studies have tested the relationships among forgiveness, social support, and PTG; however, a mechanism of social support has not been completely explored in hemodialysis patients.ObjectiveTo test the relationship between forgiveness and post-traumatic growth and verify the mediating factor of social support on the relationship between forgiveness and PTG in hemodialysis patients.Materials and methodsIn a descriptive cross-sectional study using convenience sampling from March to May 2021, 497 hemodialysis patients from nine hospitals filled out the Perceived Social Support Scale (PSSS), Heartland Forgiveness Scale (HFS), Post-traumatic Growth Inventory (PTGI), and general information. Data were analyzed using SPSS, and structural equation modeling was used to explore the relationships among forgiveness, social support, and PTG.ResultsForgiveness was significantly positively associated with PTG (P &lt; 0.01). The proposed model provided a good fit to the data. Social support was found to play a partial mediating role between forgiveness and PTG (a*b = 0.122, BCa 95% CI: 0.078∼0.181).ConclusionThe results imply that forgiveness significantly directly and indirectly is related to PTG. Forgiveness in hemodialysis patients should be detected and effectively managed to ameliorate positive effects on PTG. It is necessary for nurses to consider implementing forgiveness interventions with an emphasis on building social support strategies to help hemodialysis patients enhance their PTG.
Barriers and facilitators for access and utilization of reproductive and sexual health services among Female Sex Workers in urban and rural Maharashtra, India
Authors: Shewale Suhas , Sahay Seema 
Published: December 2022

BackgroundThe public health interventions among Female Sex Workers (FSWs) have mainly addressed HIV/ STI prevention. The focus of the HIV prevention program on FSWs' Reproductive and Sexual Health (RSH) has been limited, thus, rendering them at a higher risk of unintended pregnancies, delayed pregnancy detection, and utilizing unsafe abortion methods.MethodsA multistakeholder analysis was performed to study access and use of RSH services among FSWs in urban and rural India. Between January 2016 and June 2019, a qualitative grounded theory approach was used to explore the FSWs' perspectives and experiences about services pertaining to HIV prevention, Antenatal Care (ANC), child delivery, abortion, and pregnancy prevention. Using purposive and convenience sampling, 29 In-Depth Interviews (IDIs), 2 Focus Group Discussions (FGDs) and 22 Key Informant Interviews (KIIs) were conducted with consenting FSWs and indirect stakeholders, respectively. Verbatim translated data was entered in NVivo12 Software and analyzed inductively.ResultsThe following themes emerged: (1) Condomless sex, unintended pregnancy, vertical transmission, (2) Signs/ indication used for pregnancy detection causing delay (3) Pregnancy prevention methods used, (4) Pregnancy prevention or AIDS prevention, (5) Legal formalities as a barrier to access RSH, (6) Differential facility preference.ConclusionPregnancy prevention is a greater motivation for condom use than HIV prevention among FSWs. Therefore, there is an emerging need to reallocate public health resources and redesign policies to meet the RSH needs of FSWs, especially for the prevention of unintended pregnancies. FSW-focused Information Education Communication (IEC) strategies for RSH service utilization are essential to reduce the burden of unintended pregnancies. The National HIV Targeted Intervention (TI) program needs to include pregnancy testing services and information to non-barrier contraceptive methods. An ambient policy environment calls for examining the need for male involvement in pregnancy, family planning and abortion decisions.
Profiling resilience: A latent profile analysis of German nurses' coping and resilience
Authors: Klingenberg Ingo , Süß Stefan 
Published: December 2022

IntroductionBecause of the shortage of nurses, it becomes crucial for organizations and health systems to keep nurses in their workforce. As individual resilience is positively associated with organizational commitment and negatively with mental disorders, it may reduce nurses' intention to leave the profession. Thus, individual resilience gained attention in research. Nevertheless, there is no common conceptualization of individual resilience in the literature. Rather, three prevalent understandings exist. Due to these multiple understandings, the role of coping in the context of resilience remains unclear. Against this background, the aim of this study is to analyze the relationship between nurses' resilience and coping based on a person-centered approach.MethodsThis study presents a latent profile analysis based on a survey of 210 German nurses. The profiles were generated based on the Brief Resilience Scale and Brief COPE. The Perceived Workload of Nurses' Scale and sociodemographic data were considered as explanatory factors using nominal logistic regression. Further, the relation with possible consequences was tested byχ²-test using the Irritation Scale and KUT Commitment Measure.ResultsThe study identifies four different profiles of coping and resilience. The profiles “resistant” and “social-active” show rather low irritations and high organizational commitment. The “passive” profile has lower irritation scores than the “solitary” profile does, but the “passive” profile is associated with more irritation than the “resistant” or the “social-active” profile. Whereas the other profiles include characteristics of resilience, the “solitary” profile has a vulnerable nature. The analysis shows that more coordination and information problems, higher age, and not being in a leadership role are associated with a higher probability of belonging to the “solitary” profile. The chance of belonging to the “solitary” profile is significantly higher for women than for men, whereas women have a significantly lower chance of belonging to the “resistant” profile, compared to men.ConclusionThe analysis shows that the three prevalent understandings of resilience are appropriate but it also indicates that future scientific debate requires more precision in defining individual resilience. The study contributes to sharpening the definition of resilience as well as to understanding the link between coping and resilience.
An electrochemical device to control sample pH locally in Lab-on-PCB devices: An investigation into spatial resolution
Authors: Maxted Grace , Estrela Pedro , Moschou Despina 
Published: December 2022

During the recent pandemic outbreak, Lab-on-Chip devices did not manage to fully reach their potential in rapid diagnosis of pathogens, mainly due to the lack of cost-effective LoC solutions integrated with all required sample preparation modules. This paper presents such a critical step, aiming to translate electrochemical pH control into practical protein preconcentration modules, easy to integrate with subsequent quantification modules seamlessly via Lab-on-PCB technology. In this work we present a device capable of electrochemically controlling the pH of a solution local to an individually addressed electrode in a PCB array. The electrodes were functionalised with an electropolymerised self-assembled monolayer of 4-Aminothiophenol and were subjected to voltages of 0.2–0.4 V, evaluating for the first time the bias effect both over time and over space. This study enables for the first time the implementation of this technique for electrochemical pH control into practical Lab-on-PCB devices such as isoelectric focusing, via the informed design of such electrode arrays of appropriate size and spacing.
Less is better than more with resection of periacetabular tumors – A retrospective 16 years study and literature review
Authors: Atzmon Ran , Drexler Michael , Dolkart Oleg , Goldstein Yariv , Dubin Jeremy , Sternheim Amir , Gortzak Yair , Bickels Jacob 
Published: December 2022

IntroductionWide resections of periacetabular tumors create a sizeable bony defect that inevitably results in severe loss of function. Reconstruction of such defects usually requires using large metal implants, a feature associated with considerable surgery extension and complications. The aim of this study is to report resection with no reconstruction of the bony defect. In this retrospective study, we reviewed a consecutive series of 16 patients diagnosed with malignant periacetabular tumors and underwent en-bloc resection without reconstructing their remaining bone defect.MethodsRecords were reviewed of 16 consecutive patients diagnosed with malignant periacetabular tumors and underwent en-bloc resection without reconstructing their remaining bony defect. Measurements included: the duration of surgery, blood loss, hemoglobin levels and the need for blood transfusions, data on other hospitalization characteristics, and intraoperative and postoperative complications.ResultsSixteen patients with malignant periacetabular bone tumors and extensive bone destruction underwent wide periacetabular tumor resection with a mean follow-up of 75 months and a mean age of 53 years. The average HOOS score was 46 (range: 20 to 76), and the mean MSTS score was 13% (range: 0 to 15). The mean operative time was 4.1 h, and the mean blood loss was 1200 ml. At their most recent follow-up, patients had a mean shortening of their operated extremity of 4.8 cm, and all could ambulate with assisting devices.ConclusionWide resection of periacetabular tumors without reconstruction provides acceptable levels of function and was associated with shorter surgical time, less blood loss and fewer postoperative complications compared to resection with reconstruction. Therefore, this approach may be considered a viable surgical option in patients with an extensive malignant periacetabular.Level IIIRetrospective study.
Dual-beam room-temperature atomic magnetometer with high sensitivity and large dynamic range
Authors: Liu Bei , Peng Jin , Wang Hai-Ning , Fang Chang-Feng , Wang Jun-Lei , Zhao Xian , Deng Lu 
Published: December 2022

Abstract
We demonstrate a dual-beam high-sensitivity room-temperature atomic magnetometer (AM) with a large dynamic range based on a nonlinear magneto-optical rotation (NMOR) enhancing scheme. Using an inelastic wave-mixing laser, we find significant NMOR signal enhancement compared with a single-beam scheme under the same far-detuned operation conditions. This dual-beam scheme is shown to operate well in the regime of lower laser intensity and off-resonance detuning. The best sensitivity achieved is 20 fT/Hz1/2 with a bandwidth of 2 kHz, substantially greater than common spin-exchange relaxation-free AMs. This provides a way to develop low-power, inexpensive miniaturized AMs for wide application.