bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenylpropanoids in Silybum marianum cultures treated with cyclodextrins coated with magnetic nanoparticles
Authors: Corchete Purificación , Almagro Lorena , Gabaldón Jose Antonio , Pedreño María Angeles , Palazón Javier 
Published: March 2022

Abstract
The glucose oligosaccharide-derived cyclodextrins (CDs) are used for improving bioactive compound production in plant cell cultures because, in addition to their elicitation activity, CDs promote product removal from cells. However, despite these advantages, the industrial application of CDs is hampered by their high market price. A strategy to overcome this constraint was recently tested, in which reusable CD polymers coated with magnetic Fe3O4 nanoparticles were harnessed in Vitis vinifera cell cultures to produce t-resveratrol (t-R).
In this study, we applied hydroxypropyl-β-CDs (HPCD) and HPCDs coated with magnetic nanoparticles (HPCD-EPI-MN) in methyl jasmonate (MJ)-treated transgenic Silybum marianum cultures ectopically expressing either a stilbene synthase gene (STS) or a chalcone synthase gene (CHS), and compared their effects on the yields of t-R and naringenin (Ng), respectively. HPCD-EPI-MN at 15 g/L stimulated the accumulation of metabolites in the culture medium of the corresponding transgenic cell lines, with up to 4 mg/L of t-R and 3 mg/L of Ng released after 3 days. Similar amounts were produced in cultures treated with HPCD. Concentrations higher than 15 g/L of HPCD-EPI-MN and prolonged incubation periods negatively affected cell growth and viability in both transgenic cell lines. Reutilization of HPCD-EPI-MN was possible in three elicitation cycles (72 h each), after which the polymer retained 25–30% of its initial efficiency, indicating good stability and reusability.
Due to their capacity to adsorb metabolites and their recyclability, the application of magnetic CD polymers may reduce the costs of establishing efficient secondary metabolite production systems on a commercial scale.

Key points
• Long-term transgenic S. marianum suspensions stably produce transgene products
• t-R and Ng accumulated extracellularly in cultures elicited with HPCD and HPCD-EPI-MN
• The recyclability of HPCD-EPI-MN for metabolite production was proven

Graphical abstract
Drying tendency over the southern slope of the Tibetan Plateau in recent decades: role of a CGT-like atmospheric change
Authors: Wang Ziqian , Yang Song , Luo Haolin , Li Jiandong 
Published: March 2022

AbstractA significant drying tendency over the southern slope of the Tibetan Plateau (SSTP) in summer (especially in July–September) during 1980–2018 is identified in this study. Moisture budget analysis reveals that the drying tendency is dominated by a decreased vertical moisture advection due to weakened upward motion, which is mainly resulted from an anticyclonic trend appeared over the northeastern TP. This anomalous anticyclone can weaken the upper-tropospheric divergence pumping over the SSTP. In addition, moist static energy diagnosis indicates that the southern branch of the anomalous anticyclone advects low moist enthalpy air into the SSTP, which also suppresses local upward motion and convection. Moreover, the anticyclonic trend over the northeastern TP is found not a local phenomenon, but is rather associated with the large-scale atmospheric change in the middle latitudes that shows a circumglobal teleconnection (CGT)-like pattern. Our results highlight that the long-term CGT-like trend of atmospheric circulation plays a crucial role in triggering the drying tendency over the SSTP in recent decades.
Diagnostic and prognostic value of Bcl-2 in uterine leiomyosarcoma
Authors: Travaglino Antonio , Raffone Antonio , Raimondo Diego , Gencarelli Annarita , Esposito Italia , Gallo Cinzia , Improda Francesco Paolo , Vitale Salvatore Giovanni , Mollo Antonio , Casadio Paolo , Seracchioli Renato , Zullo Fulvio , Insabato Luigi 
Published: March 2022

Abstract
Background
Uterine leiomyosarcoma (uLMS) may show loss of expression of B-cell lymphoma-2 (Bcl-2) protein. It has been suggested that Bcl-2 loss may both be a diagnostic marker and an unfavorable prognostic marker in uLMS.

Objective
To define the diagnostic and prognostic value of Bcl-2 loss in uLMS through a systematic review and meta-analysis.

Methods
Electronic databases were searched from their inception to May 2020 for all studies assessing the diagnostic and prognostic value of Bcl-2 loss of immunohistochemical expression in uLMS. Data were extracted to calculate odds ratio (OR) for the association of Bcl-2 with uLMS vs leiomyoma variants and smooth-muscle tumors of uncertain malignant potential (STUMP), and hazard ratio (HR) for overall survival; a p value < 0.05 was considered significant.

Results
Eight studies with 388 patients were included. Loss of Bcl-2 expression in uLMS was not significantly associated with a diagnosis of uLMS vs leiomyoma variants and STUMP (OR = 2.981; p = 0.48). Bcl-2 loss was significantly associated with shorter overall survival in uLMS (HR = 3.722; p = 0.006). High statistical heterogeneity was observed in both analyses.

Conclusion
Loss of Bcl-2 expression appears as a significant prognostic but not diagnostic marker in uLMS. The high heterogeneity observed highlights the need for further research and larger studies.
Evaluation of the benefit of thermal spa therapy in plaque psoriasis: the PSOTHERMES randomized clinical trial
Authors: Beylot-Barry Marie , Mahé Emmanuel , Rolland Carole , de la Bretèque Maud Amy , Eychenne Claire , Charles Julie , Payen Catherine , Machet Laurent , Vermorel Céline , Foote Alison , Roques Christian , Bosson Jean-Luc 
Published: March 2022

Abstract
Spa therapy is considered an add-on treatment for psoriasis, but without any objective evaluation in the absence of randomized trials. This multicenter, open-label, randomized trial compared immediate spa therapy versus a control group having usual treatments until study assessments at 4.5 months. Spa therapy was proposed in five French spa resorts with standardized programs. Inclusion criteria were adults with plaque psoriasis, Dermatology Life Quality Index (DLQI) > 10, and stable medical treatment in the last 6 months. The main objective was DLQI ≤ 10 at 4.5 months after inclusion. VQ-Dermato and EQ5D-3L also assessed quality of life (QoL), Perceived Stress Scale (PSS) stress, and visual analogue scales (VAS) pain and pruritus. Between January 2015 and November 2018, 128 patients were randomized to either immediate spa therapy (64) (within 34 days, median) or usual treatments (61) until assessment at 4.5 months. Most were first-time spa users (71.2%). Mean DLQI and Psoriasis Area and Severity Index at inclusion were 16.7 and 10.5, respectively. Immediate spa therapy patients achieved the primary objective for 66.1% [95% CI 52.6% > 77.9%] vs 41.4% [95% CI 28.6% > 55.1%] control group patients (p = 0.007). VQ-Dermato scores and pruritus VAS significantly improved. Outcomes at 12-month follow-up of the immediate spa therapy group showed persistent improvement of DLQI, VQ-Dermato, and pruritus. This randomized controlled trial demonstrated that a cure of spa therapy improves QoL and alleviates certain symptoms of psoriasis, in short and long terms. This justifies its integration in the therapeutic strategies for psoriasis. Trial registration number: ClinicalTrials.gov Identifier: NCT02098213.
AI-based user authentication reinforcement by continuous extraction of behavioral interaction features
Authors: Garabato Daniel , Dafonte Carlos , Santoveña Raúl , Silvelo Arturo , Nóvoa Francisco J. , Manteiga Minia 
Published: March 2022

AbstractIn this work, we conduct an experiment to analyze the feasibility of a continuous authentication method based on the monitorization of the users’ activity to verify their identities through specific user profiles modeled via Artificial Intelligence techniques. In order to conduct the experiment, a custom application was developed to gather user records in a guided scenario where some predefined actions must be completed. This dataset has been anonymized and will be available to the community. Additionally, a public dataset was also used for benchmarking purposes so that our techniques could be validated in a non-guided scenario. Such data were processed to extract a number of key features that could be used to train three different Artificial Intelligence techniques: Support Vector Machines, Multi-Layer Perceptrons, and a Deep Learning approach. These techniques demonstrated to perform well in both scenarios, being able to authenticate users in an effective manner. Finally, a rejection test was conducted, and a continuous authentication system was proposed and tested using weighted sliding windows, so that an impostor could be detected in a real environment when a legitimate user session is hijacked.
Formal model for inter-component communication and its security in android
Authors: El-Zawawy Mohamed A. , Faruki Parvez , Conti Mauro 
Published: March 2022

AbstractThe Android application framework has a pervasive presence. In early 2021, Android has over $$70\%$$

70
%

share of the operating system mobile market (according to GlobalStats). Components are the main building blocks of Android Applications. These blocks communicate via a rich Inter-Component Communication (ICC) model rather than the traditional inter-process communication model. , -filters, and their resolution (matching) algorithm are main elements of the ICC. However, the resolution algorithm is not robust enough and has flaws that can lead to security breaches. In this paper, we present DLAIR, as an enrichment of the resolution algorithm to overcome its security issues. To this end, we start by presenting a formal model to express and validate the ICC semantics. This includes defining key properties guaranteeing consistent and realistic semantic states. We then demonstrate how the semantics can be used to formally validate ICC aspects and to express and check ICC system updates. We verified our proposed model and all its lemmas and theorems in the Coq Proof Assistant, a machine-assisted verification tool. We extend our semantics to develop DLAIR which is assisted by a heuristic, and lightweight tool, LekInt. This tool identifies suspicious execution paths responsible for based sensitive user-information leakage. On a dataset of 2000 real-world apps, we evaluated LekInt against Flowdroid, a state-of-the-art information leakage analysis tool. Experiments show that LekInt is more effective and efficient than Flowdroid which has a higher false-negative rate and lower false-positive rate than LekInt. Considering the dynamic context in which LekInt is designed to work, the advantage of efficiency overcomes the disadvantage of higher false-negative.
Comparing future climatic suitability to shoreline loss for recreational beach use: a case study of five Japanese beaches
Authors: Zajch Andrew , Hewer Micah J. , Gough William A. , Udo Keiko 
Published: March 2022

AbstractCoastal tourism is impacted by regional environmental change, including sea-level rise and climatic change. A case study at five Japanese beaches was conducted to determine the relative difference in coastal and climatic changes that are important for recreational beach tourism. Future climate conditions and projections of shoreline changes due to sea-level rise were estimated using ensembles of 14 and 21 Global Circulation Models (GCMs), respectively. The Holiday Climate Index (HCI:Beach) specific to recreational beach use was used to assess historical and future climatic suitability while beach loss area was used to quantify physical changes to beach conditions. Diminished beaches with improved climate conditions were observed at all sites except Yonehara, which had both diminished beach and climate conditions. Comparing HCI:Beach scores relative to historical conditions revealed a change of −2.4 to +10%, while beach losses were typically >60%. These results highlight that beach tourism suitability at the study sites will likely exhibit greater changes due to sea-level rise, compared to those driven by changing atmospheric conditions.
STREETS AND OPEN SPACES: COMPARING MOBILITY AND URBAN FORM AT ANGAMUCO AND CHUNCHUCMIL, MEXICO
Authors: Hutson Scott R. , Solinis-Casparius Rodrigo 
Published: March 2022

AbstractAngamuco and Chunchucmil are two of the few Mesoamerican cities with relatively complete street maps. These maps provide a rare opportunity to study how the bulk of the population moved through cities, how people worked together to organize a network of paths and open spaces, what kind of interactions these features afforded, and how they contributed to the formation of social identities. Having found that space syntax methods confirmed intuitive understandings without generating new findings, we apply a segment (paths) and node (intersections) analysis to both sites. With these analyses we recorded and characterized segment variables such as width, length, form, and curvature, and node variables such as size, form, and number of linked segments. Many of the nodes at both sites are open spaces, allowing us to register details about the configuration of shared public spaces that are less formal than monumental plazas. The analyses revealed neighborhood differentiation, local-level coordination of labor, and intentional efforts to create markets or spaces of assembly that may have complemented collective governance proposed for both sites. While Angamuco and Chunchucmil differ in terms of the general pattern of their pedestrian networks, they share similarities in terms of density of paths and types of intersections.
Novel circularly polarized slot array antennas with a wide 3-dB axial ratio bandwidth based on a spindle-shaped radiating cavity
Authors: Wang Hai , Lei Xue , Duan Tiandong , Li Tianpeng , Gao Jun , Zhao Mingyang 
Published: March 2022

AbstractA novel wide 3-dB axial ratio (AR) circularly polarized 2 × 2 array antenna is proposed in this paper. The spindle-shaped coupling cavity with tilted waveguide is capable of generating circular polarization waves from incident linear waves, which improves the AR bandwidth (ARBW) of the antenna. With this structure, a similar amplitude of the two orthogonal transmitted wave components and a stable phase difference of nearly 90° can be generated. The circularly polarized antenna proposed herein has been fabricated. According to the measurement results, the operating bandwidth from 5.32 to 6.13 GHz is <−10 dB. In addition, the measured ARBW, which is below 3 dB, can cover the range of 5.41–6.02 GHz. The maximum gain of the antenna can attain 15.65 dBi, and the efficiency is better than 80%.
The Demands-Buffering Role of Perceived and Received Social Support	for Perceived Stress and Cortisol Levels
Authors: Schmiedl Annika , Schulte Eva-Maria , Kauffeld Simone 
Published: March 2022

Abstract. Background: Social support is known as a crucial resource in buffering the effect of stress in terms of negative outcomes. Nevertheless, research on potential buffering effects of support before stress sets in has mostly entailed experimental studies. Thus, the current literature limits the transferability into the field, especially concerning the different roles of perceived and actually received social support. Aim: This study aims to extend research on the mechanisms behind the demands-buffering effects of social support. Accordingly, the job demands-resources framework undergirded our analysis of the effects of different aspects of social support (perceived vs. received support and support quality vs. quantity) on the relationship between demands and perceived stress as well as cortisol levels. Method: Data were collected from N = 125 participants at two measurement points 4 weeks apart, using questionnaires, social network analysis, and salivary cortisol. Results: Study findings reveal that (1) buffering effects of support were different for perceived stress and cortisol levels; (2) the buffering effect of perceived support depended on the level of demands, how stress was measured, and whether received support was included in the model; and (3) support quality demonstrated a demand-buffering effect, while support quantity showed contradictory patterns. Limitations: Limitations concerning the sample characteristics and measurement approaches are discussed. Conclusion: Overall, received support, especially quality, seems most relevant for buffering the effect of demands on stress. Moreover, the findings emphasize the need to assess social support as a multidimensional construct to better understand the mechanism of its demand-buffering effects.
Incorporating patient perspectives in the development of a core outcome set for reproductive genetic carrier screening: a sequential systematic review
Authors: Richardson Ebony , McEwen Alison , Newton-John Toby , Crook Ashley , Jacobs Chris 
Published: March 2022

AbstractThere is currently no consensus on the key outcomes of reproductive genetic carrier screening (RGCS). This has led to a large amount of variability in approaches to research, limiting direct comparison and synthesis of findings. In a recently published systematic review of quantitative studies on RGCS, we found that few studies incorporated patient-reported outcomes. In response to this gap, we conducted a sequential systematic review of qualitative studies to identify outcomes exploring the patient experience of RGCS. In conjunction with the review of quantitative studies, these outcomes will be used to inform the development of a core outcome set. Text excerpts relevant to outcomes, including quotes and themes, were extracted verbatim and deductively coded as outcomes. We conducted a narrative synthesis to group outcomes within domains previously defined in our review of quantitative studies, and identify any new domains that were unique to qualitative studies. Seventy-eight outcomes were derived from qualitative studies and grouped into 19 outcome domains. Three new outcome domains were identified; ‘goals of pre- and post-test genetic counselling’, ‘acceptability of further testing and alternative reproductive options’, and ‘perceived utility of RGCS’. The identification of outcome domains that were not identified in quantitative studies indicates that outcomes reflecting the patient perspective may be under-represented in the quantitative literature on this topic. Further work should focus on ensuring that outcomes reflect the real world needs and concerns of patients in order to maximise translation of research findings into clinical practice.
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing
Authors: Goenka Sneha D. , Gorzynski John E. , Shafin Kishwar , Fisk Dianna G. , Pesout Trevor , Jensen Tanner D. , Monlong Jean , Chang Pi-Chuan , Baid Gunjan , Bernstein Jonathan A. , Christle Jeffrey W. , Dalton Karen P. , Garalde Daniel R. , Grove Megan E. , Guillory Joseph , Kolesnikov Alexey , Nattestad Maria , Ruzhnikov Maura R. Z. , Samadi Mehrzad , Sethia Ankit , Spiteri Elizabeth , Wright Christopher J. , Xiong Katherine , Zhu Tong , Jain Miten , Sedlazeck Fritz J. , Carroll Andrew , Paten Benedict , Ashley Euan A. 
Published: March 2022

AbstractWhole-genome sequencing (WGS) can identify variants that cause genetic disease, but the time required for sequencing and analysis has been a barrier to its use in acutely ill patients. In the present study, we develop an approach for ultra-rapid nanopore WGS that combines an optimized sample preparation protocol, distributing sequencing over 48 flow cells, near real-time base calling and alignment, accelerated variant calling and fast variant filtration for efficient manual review. Application to two example clinical cases identified a candidate variant in <8 h from sample preparation to variant identification. We show that this framework provides accurate variant calls and efficient prioritization, and accelerates diagnostic clinical genome sequencing twofold compared with previous approaches.
Evaluating the usefulness of VGI from Waze for the reporting of flash floods
Authors: Lowrie Chris , Kruczkiewicz Andrew , McClain Shanna N. , Nielsen Miriam , Mason Simon J. 
Published: March 2022

AbstractUsing volunteered geographic information (VGI) to supplement disaster risk management systems, including forecasting, risk assessment, and disaster recovery, is increasingly popular. This attention is driven by difficulties in detection and characterization of hazards, as well as the rise of VGI appropriate for characterizing specific forms of risk. Flash-flood historical records, especially those that are impact-based, are not comprehensive, leading to additional barriers for flash-flood research and applications. In this paper we develop a method for associating VGI flood reporting clusters against authoritative data. Using Hurricane Harvey as a case study, VGI reports are assimilated into a spatial analytic framework that derives spatial and temporal clustering parameters supported by associations between Waze’s community-driven emergency operations center and authoritative reports. These parameters are then applied to find previously unreported likely flash flood-events. This study improves the understanding of the distribution of flash flooding during Hurricane Harvey and shows potential application to events in other areas where Waze data and reporting from official sources, such as the National Weather Service, are available.
Beeinträchtigt die robotergestützte radikale Zystektomie das onkologische Ergebnis bei Blasenkrebspatienten?
Authors: Mally David , Pfister David , Heidenreich Axel , Albers Peter , Niegisch Günter 
Published: March 2022

ZusammenfassungDas Einsatzgebiet der roboterassistierten Chirurgie in der Urologie umfasst mittlerweile ein breites Spektrum an Eingriffen. Wesentlich sind dabei neben rekonstruktiven Eingriffen insbesondere tumorchirurgische Eingriffe wie die radikale Zystektomie. Während Untersuchungen zur perioperativen Qualität der robotisch-assistierten radikalen Zystektomie einen günstigen Einfluss auf die Morbidität (und damit auch Mortalität) nahelegen, bleibt die Frage, inwieweit auch die onkologische Qualität des Eingriffs gemessen am rezidivfreien und Gesamtüberleben beeinflusst.In diesem Kontext werden in dieser Arbeit nach einer umfassenden Literaturrecherche sowohl die Daten retrospektive Kohortenvergleiche zwischen offener und robotischer Zystektomie, Registerdaten zur robotischen Zystektomie sowie die Ergebnisse prospektiv randomisierter Studien dargestellt.Zusammengefasst ist unter onkologischen Gesichtspunkten die robotische der offenen Zystektomie nicht unterlegen. Die Daten des Gesamtüberlebens, des karzinomspezifischen Überlebens und des progressionsfreien Überlebens unterscheiden sich weder in den retrospektiven Kohortestudien, im indirekten Vergleich von Registerdaten und/oder großer Serien noch in den bisherigen prospektiven Untersuchungen. Im Hinblick auf das Auftreten atypische Metastasen nach robotischer Zystektomie fehlen aktuell prospektive Daten, dies nicht das generelle onkologische Outcome zu beeinflussen.Damit ist die robotisch-assistierte Zystektomie auch aus onkologischen Gesichtspunkten eine gute Option für Patienten, bei denen eine Indikation zur radikalen Zystektomie besteht.
Pflege als Impulsgeber?
Authors: Kasper Nico 
Published: March 2022

ZusammenfassungUrsprünglich stammt der Begriff Strategie vom griechischen „Strategos“ ab, der Kunst der Heerführung. Damit ist die Planung und Organisation von Feldzügen sowie die Einbettung einzelner taktischer Schritte in einen längerfristigen Plan mit klarer Zielsetzung gemeint. Aufs Krankenhaus gemünzt bedeutet Strategie, die Zukunft des Hauses aktiv zu gestalten und nicht nur zu reagieren.
Zurück in die Vergangenheit?
Authors: Welzbacher Marcel 
Published: March 2022

ZusammenfassungEin Blick in unsere Gesundheitseinrichtungen reicht, um festzustellen, dass sich die Lage zum Thema Personalbemessung seit 1990 kaum weiterentwickelt hat. Ist die PPR 2.0 jetzt ein Schritt in die Vergangenheit?