bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hand‐Assisted Versus Pure Minimally‐Invasive Distal Pancreatectomy: Is There a Downside to Lending a Hand?
Authors: Miller Phoebe N. , Romero‐Hernandez Fernanda , Conroy Patricia , Calthorpe Lucia , Yilma Mignote , Mohamedaly Sarah , Kelly Yvonne M. , Feng Jean , Hirose Kenzo , Kirkwood Kimberly , Maker Ajay V. , Corvera Carlos , Nakakura Eric , Alseidi Adnan , Adam Mohamed A. 
Published: November 2022

AbstractBackgroundHand‐assisted laparoscopic distal pancreatectomy (HALDP) is suggested to offer similar outcomes to pure laparoscopic distal pancreatectomy (LDP). However, given the longer midline incision, it is unclear whether HALDP increases the risk of postoperative hernia. Our aim was to determine the risk of postoperative incisional hernia development after HALDP.MethodsWe retrospectively collected data from patients undergoing HALDP or LDP at a single center (2012–2020). Primary endpoints were postoperative incisional hernia and operative time. All patients had at minimum six months of follow‐up. Outcomes were compared using unadjusted and multivariable regression analyses.ResultsNinety‐five patients who underwent laparoscopic distal pancreatectomy were retrospectively identified. Forty‐one patients (43%) underwent HALDP. Patients with HALDP were older (median, 67 vs. 61 years,p = 0.02). Sex, race, Body Mass Index (median, 27 vs. 26), receipt of neoadjuvant chemotherapy, gland texture, wound infection rates, postoperative pancreatic fistula, overall complications, and hospital length‐of‐stay were similar between HALDP and LDP (allp > 0.05). In unadjusted analysis, operative times were shorter for HALDP (164 vs. 276 min,p < 0.001), but after adjustment, did not differ significantly (MR 0.73; 0.49–1.07,p = 0.1). Unadjusted incidence of hernia was higher in HALDP versus LDP (60% vs. 24%,p = 0.004). After adjustment, HALDP was associated with an increased odds of developing hernia (OR 7.52; 95% CI 1.54–36.8,p = 0.014). After propensity score matching, odds of hernia development remained higher for HALDP (OR 4.62; 95% CI 1.28–16.65, p = 0.031)p = 0.03).ConclusionsCompared with LDP, HALDP was associated with increased likelihood of postoperative hernia with insufficient evidence that HALDP shortens operative times. Our results suggest that HALDP may not be equivalent to LDP.
Modulation Theory for Radially Symmetric Kink Waves Governed by a Multi-Dimensional Sine-Gordon Equation
Authors: Nguyen Lu Trong Khiem , Smyth Noel Frederick 
Published: November 2022

AbstractWe derive a modulation theory for the resolution of radially symmetric kink waves governed by a multi-dimensional sine-Gordon equation. Whitham modulation theory is developed to explain the return of an expanding kink wave, as well as predicting its maximum expansion radius and its return time. Comparisons with full numerical solutions of the sine-Gordon equation show that the modulation theory gives excellent predictions for not only the returning time and the maximum expansion radius, but also for the details of the kink itself. In addition, the method can be extended to dissipative sine-Gordon equations and generalized to deal with a wide class of initial conditions beyond kinks.
Comparative single-cell analysis of the adult heart and coronary vasculature
Authors: Balachandran Saranya , Pozojevic Jelena , Sreenivasan Varun K. A. , Spielmann Malte 
Published: November 2022

AbstractThe structure and function of the circulatory system, including the heart, have undergone substantial changes with the vertebrate evolution. Although the basic function of the heart is to pump blood through the body, its size, shape, speed, regeneration capacity, etc. vary considerably across species. Here, we address the differences among vertebrate hearts using a single-cell transcriptomics approach. Published datasets of macaque (Macaca fascicularis), mouse, and zebrafish hearts were integrated and compared to the human heart as a reference. While the three mammalian hearts integrated well, the zebrafish heart showed very little overlap with the other species. Our analysis revealed a mouse-specific cell subpopulation of ventricular cardiomyocytes (CM), represented by strikingly different expression patterns of specific genes related to high-energy metabolism. Interestingly, the observed differences between mouse and human CM coincided with actual biological differences between the two species. Smooth muscle and endothelial cells (EC) exhibited species-specific differences in clustering and gene expression, respectively, which we attribute to the tissues selected for sequencing, given different focuses of the original studies. Finally, we compared human and zebrafish heart-specific fibroblasts (FB) and identified a distinctively high expression of genes associated with heart regeneration following injury in zebrafish. Together, our results show that integration of numerous datasets of different species and different sequencing technologies is feasible and that this approach can identify species-specific differences and similarities in the heart.
Impacts of ocean warming on the settlement success and post-settlement survival of Pacific crown-of-thorns starfish (Acanthaster cf. solaris)
Authors: Lang Bethan J. , Caballes Ciemon F. , Uthicke Sven , Doll Peter C. , Donelson Jennifer M. , Pratchett Morgan S. 
Published: November 2022

AbstractOcean warming and population irruptions of crown-of-thorns starfish (CoTS; Acanthaster cf. solaris) are two of the greatest threats to coral reefs. As such, there is significant interest in understanding how CoTS may be directly impacted by rising ocean temperatures. Settlement of planktonic larvae and subsequent metamorphosis is purported to be a major population bottleneck in marine invertebrates, yet it is unknown how ocean warming will impact these processes in CoTS. Herein, the effect of temperature (28 °C ambient, 30 °C, 32 °C, 34 °C) on the settlement success, metamorphic success, and post-settlement survival of this corallivore was explored. While larval settlement was robust to elevated temperature, with at least 94% of larvae settling after 48 h across all temperatures, it was observed that settlement success was lower on substrate that had been pre-treated ≥ 32 °C. Metamorphic success was also significantly constrained at temperatures ≥ 32 °C. At 32 °C and 34 °C metamorphic success was 16% and 63% lower than at ambient temperature, respectively. Significant adverse effects of warming on post-settlement survival were observed at even cooler temperatures, with 10% lower survival at 30 °C compared to at ambient temperature, and at 34 °C, survival was 34% lower. Substantial reductions in metamorphic success and early post-settlement survival at elevated temperatures, as well as negative impacts of warming on the settlement substrate and its capacity to induce settlement, may present a bottleneck for recruitment in a warmer ocean.
Trends and patterns of initial percutaneous nephrolithotomy and subsequent procedures among commercially-insured US adults with urinary system stone disease: a 10-year population-based study
Authors: Johnston Stephen S. , Johnson Barbara H. , Rai Pragya , Grange Philippe , Amos Tony , Ghosh Sudip , Buchholz Noor 
Published: November 2022

Abstract
Purpose
To describe trends and patterns of initial percutaneous nephrolithotomy (PCNL) and subsequent procedures from 2010 to 2019 among commercially-insured US adults with urinary system stone disease (USSD).

Methods
Retrospective study of administrative data from the IBM® MarketScan® Database. Eligible patients were aged 18–64 years and underwent PCNL between 1/1/2010 and 12/31/2019. Measures of interest for analysis of trends and patterns included the setting of initial PCNL (inpatient vs. outpatient), percutaneous access (1 vs. 2-step), and the incidence, time course, and type of subsequent procedures (extracorporeal shockwave lithotripsy [SWL], ureteroscopy [URS], and/or PCNL) performed up-to 3 years after initial PCNL.

Results
A total of 8,348 patients met the study eligibility criteria. During the study period, there was a substantial shift in the setting of initial PCNL, from 59.9% being inpatient in 2010 to 85.3% being outpatient by 2019 (P < 0.001). The proportion of 1 vs. 2-step initial PCNL fluctuated over time, with a low of 15.1% in 2016 and a high of 22.0% in 2019 but showed no consistent yearly trend (P = 0.137). The Kaplan–Meier estimated probability of subsequent procedures following initial PCNL was 20% at 30 days, 28% at 90 days, and 50% at 3 years, with slight fluctuations by initial PCNL year. From 2010 to 2019, the proportion of subsequent procedures accounted for by URS increased substantially (from 30.8 to 51.8%), whereas SWL decreased substantially (from 39.5 to 14.7%) (P < 0.001).

Conclusions
From 2010 to 2019, PCNL procedures largely shifted to the outpatient setting. Subsequent procedures after initial PCNL were common, with most occurring within 90 days. URS has become the most commonly-used subsequent procedure type.
Direct detection and quantification of Toxoplasma gondii in meat samples from feral raccoons (Procyon lotor) in Germany by magnetic-capture real-time PCR
Authors: Engel Lydia , Hamedy Ahmad , Koethe Martin 
Published: November 2022

AbstractBecause the number of wild raccoons in Germany is increasing constantly, it appears to be economic reasonable to use their meat as food. For this purpose, it is essential to generate data regarding the pathogen load of the meat to be consumed and handled. It is known that raccoons, particularly in Germany, show a high seroprevalence ofToxoplasma gondii. Because serological data only indicates contact of a host to a parasite additional direct detection is needed to prove presence of parasitic stages in particular tissues. Therefore, a total of 150 samples from raccoons with known serostatus were tested and quantified using magnetic-capture real-time PCR forToxoplasma gondii.As it represents potentially consumption-relevant parts of raccoons, meat from forelimb and hindlimb was examined. Samples were stratified into three groups based on the animals’ serostatus (each 50 negative, low positive, and high positive). All samples from seronegative animals were found negative by MC-PCR as well. In a total of 56 meat samples from 100 seropositive animals,T. gondiiDNA was detected. Statistically significant more samples were positive by MC-PCR in the high positive than in the low positive serostatus group (38/50 vs. 18/50,p < 0.0001). Furthermore, samples from the former group were also found to have statistically significant higher DNA equivalent values compared to samples from the low positive serostatus group (p < 0.0001). These results suggest that meat from seropositive raccoons may contain considerable numbers ofT. gondiipresenting a potential public health risk for humans whilst handling and consumption.
A re-appraisal of mesenchymal-epithelial transition (MET) in endometrial epithelial remodeling
Authors: Spooner-Harris Madelyn , Kerns Karl , Zigo Michal , Sutovsky Peter , Balboula Ahmed , Patterson Amanda L. 
Published: November 2022

Abstract
Mesenchymal-epithelial transition (MET) is a mechanism of endometrial epithelial regeneration. It is also implicated in adenocarcinoma and endometriosis. Little is known about this process in normal uterine physiology. Previously, using pregnancy and menses-like mouse models, MET occurred only as an epithelial damage/repair mechanism. Here, we hypothesized that MET also occurs in other physiological endometrial remodeling events, outside of damage/repair, such as during the estrous cycle and adenogenesis (gland development). To investigate this, Amhr2-Cre-YFP/GFP mesenchyme-specific reporter mice were used to track the fate of mesenchymal-derived (MD) cells. Using EpCAM (epithelial marker), EpCAM+YFP+ MD-epithelial cells were identified in all stages of the estrous cycle except diestrus, in both postpartum and virgin mice. EpCAM+YFP+ MD-epithelial cells comprised up to 80% of the epithelia during estrogen-dominant proestrus and significantly declined to indistinguishable from control uteri in diestrus, suggesting MET is hormonally regulated. MD-epithelial cells were also identified during postnatal epithelial remodeling. MET occurred immediately after birth at postnatal day (P) 0.5 with EpCAM+GFP+ cells ranging from negligible (0.21%) to 82% of the epithelia. EpCAM+GFP+ MD-epithelial cells declined during initiation of adenogenesis (P8, avg. 1.75%) and then increased during gland morphogenesis (P14, avg. 10%). MD-epithelial cells expressed markers in common with non-MD-epithelial cells (e.g., EpCAM, FOXA2, ESR1, PGR). However, MD-epithelial cells were differentially regulated postnatally and in adults, suggesting a functional distinction in the two populations. We conclude that MET occurs not only as an epithelial damage/repair mechanism but also during other epithelial remodeling events, which to our knowledge has not been demonstrated in other tissues.
Hawk owl irruptions: spatial and temporal variation in rodent abundance drive push and pull dynamics
Authors: Dale Svein , Sonerud Geir A. 
Published: November 2022

AbstractBird irruptions are thought to be triggered by low food availability in breeding areas, thereby causing emigration (push factor). However, few studies have tested whether emigrating individuals are drawn towards areas of high food availability (pull factor). The Northern hawk owl (Surnia ulula), a rodent specialist, occurs irruptively to southern parts of Fennoscandia. We analysed whether irruption size during 1980–2020 in southeastern Norway was related to rodent abundance at four sites 450–990 km to the north-northeast (potential source areas) and at two sites in southeastern Norway to test push and pull dynamics of irruptions. Irruptions occurred when rodent abundance in potential source areas were low, supporting the push hypothesis. High rodent abundance in potential source areas 1–2 years before irruptions suggested that irruptions were preceded by high reproduction. Upon arrival to southeastern Norway, hawk owls did not encounter high rodent abundance in their main habitat (boreal forest). However, hawk owls stayed in boreal forest in hills in years with higher microtine rodent abundance, but occurred in farmland areas in the lowlands when microtine rodents were less abundant. Use of lowlands coincided with higher than median numbers of wood mouse (Apodemus sylvaticus) for 87% of the hawk owls settling in the lowlands, thus suggesting support for the pull hypothesis. In conclusion, hawk owl irruptions to southern Fennoscandia were triggered by low food availability in northern areas (push factor), and appeared to be drawn by high food availability in southeastern Norway to some degree (pull factor).
Effects of add-on Celecoxib treatment on patients with schizophrenia spectrum disorders and inflammatory cytokine profile trial (TargetFlame): study design and methodology of a multicentre randomized, placebo-controlled trial
Authors: Strube Wolfgang , Aksar Aslihan , Bauer Ingrid , Barbosa Susana , Benros Michael , Blankenstein Christiane , Campana Mattia , Davidovic Laetitia , Glaichenhaus Nicolas , Falkai Peter , Görlitz Thomas , Hansbauer Maximilian , Heilig Daniel , Khalfallah Olfa , Leboyer Marion , Martinuzzi Emanuela , Mayer Susanne , Moussiopoulou Joanna , Papazova Irina , Perić Natasa , Wagner Elias , Schneider-Axmann Thomas , Simon Judit , Hasan Alkomiet 
Published: November 2022

AbstractNeuroinflammation has been proposed to impact symptomatology in patients with schizophrenia spectrum disorders. While previous studies have shown equivocal effects of treatments with add-on anti-inflammatory drugs such as Aspirin,N-acetylcysteine and Celecoxib, none have used a subset of prospectively recruited patients exhibiting an inflammatory profile. The aim of the study is to evaluate the efficacy and safety as well as the cost-effectiveness of a treatment with 400 mg Celecoxib added to an ongoing antipsychotic treatment in patients with schizophrenia spectrum disorders exhibiting an inflammatory profile. The “Add-on Celecoxib treatment in patients with schizophrenia spectrum disorders and inflammatory cytokine profile trial (TargetFlame)” is a multicentre randomized, placebo-controlled phase III investigator-initiated clinical trial with the following two arms: patients exhibiting an inflammatory profile receiving either add-on Celecoxib 400 mg/day or add-on placebo. A total of 199 patients will be assessed for eligibility by measuring blood levels of three pro-inflammatory cytokines, and 109 patients with an inflammatory profile, i.e. inflamed, will be randomized, treated for 8 weeks and followed-up for additional four months. The primary endpoint will be changes in symptom severity as assessed by total Positive and Negative Syndrome Scale (PANSS) score changes from baseline to week 8. Secondary endpoints include various other measures of psychopathology and safety. Additional health economic analyses will be performed. TargetFlame is the first study aimed at evaluating the efficacy, safety and cost-effectiveness of the antiphlogistic agent Celecoxib in a subset of patients with schizophrenia spectrum disorders exhibiting an inflammatory profile. With TargetFlame, we intended to investigate a novel precision medicine approach towards anti-inflammatory antipsychotic treatment augmentation using drug repurposing.Clinical trial registration:http://www.drks.de/DRKS00029044andhttps://trialsearch.who.int/Trial2.aspx?TrialID=DRKS00029044
Forecasting seasonal to sub-seasonal rainfall in Great Britain using convolutional-neural networks
Authors: Barnes Andrew Paul , McCullen Nick , Kjeldsen Thomas Rodding 
Published: November 2022

AbstractTraditional weather forecasting approaches use various numerical simulations and empirical models to produce a gridded estimate of rainfall, often spanning multiple regions but struggling to capture extreme events. The approach presented here combines modern meteorological forecasts from the ECMWF SEAS5 seasonal forecasts with convolutional neural networks (CNNs) to improve the forecasting of total monthly regional rainfall across Great Britain. The CNN is trained using mean sea-level pressure and 2-m air temperature forecasts from the ECMWF C3S service using three lead-times: 1 month, 3 months and 6 months. The training is supervised using the equivalent benchmark rainfall data provided by the CEH-GEAR (Centre for Ecology and Hydrology, gridded estimates of areal rainfall). Comparing the CNN to the ECMWF predictions shows the CNN out-performs the ECMWF across all three lead times. This is done using an unseen validation dataset and based on the root mean square error (RMSE) between the predicted rainfall values for each region and benchmark values from the CEH-GEAR dataset. The largest improvement is at a 1-month lead time where the CNN model scores a RMSE 6.89 mm lower than the ECMWF. However, these differences are exacerbated at the extremes with the CNN producing, at a 1-month lead time, RMSEs which are 28.19 mm lower than the corresponding predictions from the ECMWF. Following this, a sensitivity analysis shows the CNN model predicts increased rainfall values in the presence of a low sea-level pressure anomaly around Iceland, followed by a high sea-level pressure anomaly south of Greenland.
Screening performances of an 8-item UPSIT Italian version in the diagnosis of Parkinson’s disease
Authors: Landolfi Annamaria , Picillo Marina , Pellecchia Maria Teresa , Troisi Jacopo , Amboni Marianna , Barone Paolo , Erro Roberto 
Published: November 2022

AbstractHyposmia is a common finding in Parkinson’s disease (PD) and is usually tested through the University of Pennsylvania Smell Identification Test (UPSIT). The aim of our study is to provide a briefer version of the Italian-adapted UPSIT test, able to discriminate between PD patients and healthy subjects (HS). By means of several univariate and multivariate (machine-learning-based) statistical approaches, we selected 8 items by which we trained a partial-least-square discriminant analysis (PLS-DA) and a decision tree (DT) model: class predictions of both models performed better with the 8-item version when compared to the 40-item version. An area under the receiver operating characteristic (AUC-ROC) curve built with the selected 8 odors showed the best performance (sensitivity 86.8%, specificity 82%) in predicting the PD condition at a cut-off point of ≤ 6. These performances were higher than those previously calculated for the 40-item UPSIT test (sensitivity 82% and specificity 88.2 % with a cut-off point of ≤ 21). Qualitatively, our selection contains one odor (i.e., apple) which is Italian-specific, supporting the need for cultural adaptation of smell testing; on the other hand, some of the selected best discriminating odors are in common with existing brief smell test versions validated on PD patients of other cultures, supporting the view that disease-specific odor patterns may exist and deserve a further evaluation.
Clinical score for early diagnosis of myotonic dystrophy type 2
Authors: Ivanovic Vukan , Peric Stojan , Pesovic Jovan , Tubic Radoje , Bozovic Ivo , Petrovic Djordjevic Ivana , Savic-Pavicevic Dusanka , Meola Giovanni , Rakocevic-Stojanovic Vidosava 
Published: November 2022

Abstract
Introduction

Myotonic dystrophy type 2 (DM2) is a rare, multisystemic, autosomal dominant disease with highly variable clinical presentation. DM2 is considered to be highly underdiagnosed.

Objective
The aim of this study was to determine which symptoms, signs, and diagnostic findings in patients referred to neurological outpatient units are the most indicative to arouse suspicion of DM2. We tried to make a useful and easy-to-administer clinical scoring system for early diagnosis of DM2-DM2 early diagnosis score (DM2-EDS).

Patients and methods
Two hundred ninety-one patients with a clinical suspicion of DM2 were included: 69 were genetically confirmed to have DM2, and 222 patients were DM2 negative. Relevant history, neurological, and paraclinical data were obtained from the electronic medical records.

Results
The following parameters appeared as significant predictors of DM2 diagnosis: cataracts (beta = 0.410, p < 0.001), myotonia on needle EMG (beta = 0.298, p < 0.001), hand tremor (beta = 0.211, p = 0.001), positive family history (beta = 0.171, p = 0.012), and calf hypertrophy (beta = 0.120, p = 0.043). In the final DM2-EDS, based on the beta values, symptoms were associated with the following values: cataracts (present 3.4, absent 0), myotonia (present 2.5, absent 0), tremor (present 1.7, absent 0), family history (positive 1.4, negative 0), and calf hypertrophy (present 1.0, absent 0). A cut-off value on DM2-EDS of 3.25 of maximum 10 points had a sensitivity of 84% and specificity of 81% to diagnose DM2.

Conclusion
Significant predictors of DM2 diagnosis in the neurology outpatient unit were identified. We made an easy-to-administer DM2-EDS score for early diagnosis of DM2.