bluuebunny/crossref_metadata_2025_split huggingface dataset:
Specific gut bacterial and fungal microbiota pattern in the first half of pregnancy is linked to the development of gestational diabetes mellitus in the cohort including obese women
Authors: Vavreckova Marketa , Galanova Natalie , Kostovcik Martin , Krystynik Ondrej , Ivanovova Eliska , Roubalova Radka , Jiraskova Zakostelska Zuzana , Friedecky David , Friedecka Jaroslava , Haluzik Martin , Karasek David , Kostovcikova Klara 
Published: September 2022

AimsGestation is linked to changes in gut microbiota composition and function. Since gestational diabetes mellitus (GDM) can develop at any time of the pregnancy, we stratified the women into four groups according to the time and test used for the diagnosis. We focused on the gut microbiota pattern in early pregnancy to detect changes which could be linked to later GDM development.MethodsWe collected stool samples from 104 pregnant women including obese individuals (first trimester body mass index median was 26.73). We divided the women into four groups according to routine screening of fasting plasma glucose (FPG) levels and oral glucose tolerance test (oGTT) in the first and third trimesters, respectively. We processed the stool samples for bacterial 16S rRNA and fungal ITS1 genes sequencing by Illumina MiSeq approach and correlated the gut microbiota composition with plasma short-chain fatty acid levels (SCFA).ResultsWe found that gut bacterial microbiota in the first trimester significantly differs among groups with different GDM onset based on unweighted UniFrac distances (p=0.003). Normoglycemic women had gut microbiota associated with higher abundance of family Prevotellaceae, and order Fusobacteriales, and genus Sutterella. Women diagnosed later during pregnancy either by FGP levels or by oGTT had higher abundances of genera Enterococcus, or Erysipelotrichaceae UCG-003, respectively. We observed significant enrichment of fungal genus Mucor in healthy pregnant women whereas Candida was more abundant in the group of pregnant women with impaired oGTT. Using correlation analysis, we found that Holdemanella negatively correlated with Blautia and Candida abundances and that Escherichia/Shigella abundance positively correlated and Subdoligranulum negatively correlated with plasma lipid levels. Coprococcus, Akkermansia, Methanobrevibacter, Phascolarctobacterium and Alistipes positively correlated with acetate, valerate, 2-hydroxybutyrate and 2-methylbutyrate levels, respectively, in women with GDM.ConclusionsWe conclude that there are significant differences in the gut microbiota composition between pregnant women with and without GDM already at the early stage of pregnancy in our cohort that included also overweight and obese individuals. Specific microbial pattern associated with GDM development during early pregnancy and its correlation to plasma lipid or SCFA levels could help to identify women in higher risk of GDM development.
Effectiveness of multiple eHealth-delivered lifestyle strategies for preventing or intervening overweight/obesity among children and adolescents: A systematic review and meta-analysis
Authors: Qiu Li-Ting , Sun Gui-Xiang , Li Ling , Zhang Ji-Dong , Wang Dan , Fan Bo-Yan 
Published: September 2022

ObjectiveTo investigate the effect of multiple eHealth-delivered lifestyle interventions on obesity-related anthropometric outcomes in children and adolescents.MethodsThe Medline (via PubMed), Embase, Cochrane Library, Web of Science, CBM, VIP, CNKI, and Wanfang electronic databases were systematically searched from their inception to March 18, 2022, for randomized controlled trials (RCTs). Meta-analyses were performed to investigate the effect of multiple eHealth-delivered lifestyle interventions on obesity-related anthropometric outcomes (body mass index [BMI], BMI Z-score, waist circumference, body weight, and body fat%). Two independent investigators reviewed the studies for accuracy and completeness. All included studies were evaluated using the Cochrane Risk-of-Bias (ROB) Tool.ResultsForty trials comprising 6,403 patients were selected for the meta-analysis. The eligible trials were published from 2006 to 2022. Compared with the control group, the eHealth-intervention group was more effective in reducing BMI (weighted mean difference [WMD] = −0.32, 95% confidence interval [CI]: −0.50 to −0.13, I2 = 85.9%), BMI Z-score (WMD = −0.08, 95% CI: −0.14 to −0.03, I2 = 89.1%), waist circumference (WMD = −0.87, 95% CI: −1.70 to −0.04, I2 = 43.3%), body weight (WMD = −0.96, 95% CI: −1.55 to −0.37, I2 = 0.0%), and body fat% (WMD = −0.59, 95% CI: −1.08 to −0.10, I2 = 0.0%). The subgroup analysis showed that parental or school involvement (WMD = −0.66, 95% CI: −0.98 to −0.34), eHealth-intervention duration of &gt;12 weeks (WMD = −0.67, 95% CI: −0.96 to −0.38), and mobile-based interventions (WMD = −0.78, 95% CI: −1.13 to −0.43) had a significantly greater intervention effect size on BMI.ConclusionsThis review recommends that multiple eHealth-delivered lifestyle strategies may be useful for preventing or treating overweight and obesity among children and adolescents. However, our results should be cautiously interpreted due to certain limitations in our study.
A necroptosis -related signature for predicting prognosis and immunotherapy in hepatocellular carcinoma
Authors: Fu Xing , Yang Yuling , Zhang Xiaozhi 
Published: September 2022

Background: Hepatocellular Carcinoma (HCC) is an aggressive tumor with an inferior prognosis. Necroptosis is a new form of programmed death that plays a dual effect on the tumor. However, the role of necroptosis-related genes(NRGs) in HCC remains unknown.Methods: All datasets were downloaded from publicly available databases. The consensus clustering analysis was used to classify patients into different subtypes based on NRGs. The Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression were used to develop a prognostic signature. Tumor Immune Dysfunction and Exclusion (TIDE) was used to predict immunotherapy response.Results: The genetic and transcriptional changes of NRGs were observed in HCC. Patients were classified into three clusters based on differentially expressed NRGs, of which Cluster-3 had the worst prognosis and the highest immune infiltration. The prognostic signature was developed based on 8-NRGs, which have shown excellent prognostic performance. The high-risk group in the signature presented significantly higher immune infiltration, such as aDCs, iDCs, macrophages, and Treg, compared to the low-risk group. TMB and immune checkpoints were also higher in the high-risk group. Moreover, a lower TIDE score was observed in the high-risk group, indicating the patients with high risk-score may be suitable for immunotherapy. Via the dataset of IMvigor210, we found a higher risk score in the immunotherapy response group.Conclusion: We developed a new necroptosis-related signature for predicting prognosis with the potential to predict immunotherapy for HCC patients.
Increased risk of early-onset childhood systemic lupus erythematosus for children born to affected parents: A nationwide child-parent cohort study
Authors: Wu Chun-Hsin , Chen Chih-An , Lin Sheng-Hsiang , Weng Chia-Tse , Kuo Pao-Lin , Shieh Chi-Chang 
Published: September 2022

ObjectiveChildren of women with systemic lupus erythematosus (SLE) are at risk for childhood-onset SLE (cSLE). This study evaluated the incidence of early-onset cSLE and associated risk factors, including concomitant maternal and paternal autoimmune diseases, for these children.MethodsA population-based cohort study was conducted using national databases including the linked information of children and parents. Children of women with SLE and those of women without SLE were identified between 2004 and 2015. The cumulative cSLE incidence was estimated using the Kaplan-Meier method. The marginal Cox model was used to calculate the hazard ratio (HR) for cSLE events.ResultsA total of 4,419 singletons of women with SLE and 1,996,759 singletons of women without SLE were identified. There were 9 (0.20%) and 503 (0.03%) incident cases of early-onset cSLE for offspring of women with and without SLE, respectively (incidence rate ratio, 8.34; 95% confidence interval [CI], 3.79–15.95]. The adjusted HR of incident cSLE in children of women with SLE was 4.65 (95% CI 2.11–10.24). Other risks for cSLE included pregnancy-induced hypertension/preeclampsia/eclampsia, paternal SLE, paternal Sjögren’s syndrome (SS), and maternal SS.ConclusionsThis national child-parent cohort study demonstrated that children of women with SLE are at significantly higher risk for cSLE during early childhood. Moreover, paternal SLE and parental SS increase the risk of cSLE for offspring.
Pathogenicity and antibiotic resistance analysis of Vibrio species found in coastal water at mainly beach of Shenzhen, China
Authors: Huang Ziwei , Anokyewaa Melody Abena , Wang Junlin , Jian Jichang , Lu Yishan 
Published: September 2022

Offshore industries and resources are the links between humans and the marine ecosystem. Several risk factors are associated with beach activities. The risk of Vibrio infection at bathing beaches is often overlooked as there are virtually no regulations regarding them. In this study, we investigated the current prevalence of pathogenic Vibrio spp. on the main beaches of Shenzhen. The risk indicator levels of pathogenic Vibrio species obtained were analyzed using 17 virulence genes mainly associated with Vibrio species and are responsible for several ailments and infections. A total of 60 Vibrio strains were isolated and identified by morphological observation, evolutionary tree alignment, and biochemical testing. There was a high abundance of Vibrio in the seawater. Also, a positive correlation was observed between the presence of virulence genes and the exhibition of high pathogenicity after artificially infecting fish with some of the virulent Vibrio species. In the infection experiment, it was observed that all the zebrafish infected with MEDF7 (Vibrio alginolyticus) and JSW-YELLOW (Vibrio harveyi) died a day after injection, with varying degrees of abdominal enlargement and skin ulceration. The mortality rates for strains with medium risk and low risk were 65% and 45%, respectively. Lethal dose 50 (LD50) values were 5.67 ×104 CFU/fish, 3.72 ×105 CFU/fish, and 4.31 ×105 CFU/fish for the high-risk, medium-risk, and low-risk strains of zebrafish, respectively. The results of the antibiotic sensitivity test showed that all the six experimental strains, except JSW-YELLOW, were resistant to doxycycline and neopenicillin. In summary, our study first identified and evaluated the pathogenicity of Vibrio in the Shenzhen beach baths, serving as a scientific benchmark for Vibrio risk prevention and control as well as guidance for Vibrio diagnosis through virulence factor detection and risk classification.
Study on the relationship between the particle size distribution characteristics of ground granulated blast furnace slag and its mortar properties
Authors: He Tusheng , Xu Zengwei , Li Zaibo , Zhao Xuguang , Zhao Sanyin , Liu Yang 
Published: September 2022

This study adopted the Rosin-Rammler-Bennett (RRB) equation to model the particle size distribution (PSD) of ground granulated blast furnace slag (GGBFS) and prepared four groups of GGBFS powders with a characteristic particle size De = 8–20 μm and uniformity coefficient n = 0.9–1.4 by means of grading and combining blast furnace slag. In cases both with and without polycarboxylate superplasticizers (PCE), the fluidity and strength of the mortar were measured. The study found that without PCE, the aggregation of GGBFS had a significant negative impact on the mortar fluidity and hardened paste strength. With the addition of PCE, the properties of the mortar mainly depended on two factors, the specific surface area of the GGBFS and the differences in De and n between the GGBFS and Portland cement. At a given specific surface area of GGBFS, high hardened paste strength can be obtained through simultaneously lowering the De and increasing the n.
Machine learning based on metabolomics reveals potential targets and biomarkers for primary Sjogren’s syndrome
Authors: Wang Kai , Li Ju , Meng Deqian , Zhang Zhongyuan , Liu Shanshan 
Published: September 2022

Background: Using machine learning based on metabolomics, this study aimed to construct an effective primary Sjogren’s syndrome (pSS) diagnostics model and reveal the potential targets and biomarkers of pSS.Methods: From a total of 39 patients with pSS and 38 healthy controls (HCs), serum specimens were collected. The samples were analyzed by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry. Three machine learning algorithms, including the least absolute shrinkage and selection operator (LASSO), random forest (RF), and extreme gradient boosting (XGBoost), were used to build the pSS diagnosis models. Afterward, four machine learning methods were used to reduce the dimensionality of the metabolomics data. Finally, metabolites with significant differences were screened and pathway analysis was conducted.Results: The area under the curve (AUC), sensitivity, and specificity of LASSO, RF and XGBoost test set all reached 1.00. Orthogonal partial least squares discriminant analysis was used to classify the metabolomics data. By combining the results of the univariate false discovery rate and the importance of the variable in projection, we identified 21 significantly different metabolites. Using these 21 metabolites for diagnostic modeling, the AUC, sensitivity, and specificity of LASSO, RF, and XGBoost all reached 1.00. Metabolic pathway analysis revealed that these 21 metabolites are highly correlated with amino acid and lipid metabolisms. On the basis of 21 metabolites, we screened the important variables in the models. Further, five common variables were obtained by intersecting the important variables of three models. Based on these five common variables, the AUC, sensitivity, and specificity of LASSO, RF, and XGBoost all reached 1.00.2-Hydroxypalmitic acid, L-carnitine and cyclic AMP were found to be potential targets and specific biomarkers for pSS.Conclusion: The combination of machine learning and metabolomics can accurately distinguish between patients with pSS and HCs. 2-Hydroxypalmitic acid, L-carnitine and cyclic AMP were potential targets and biomarkers for pSS.
Impact of physiotherapy with telerehabilitation on caregivers of patients with neurological disorders: A systematic review protocol
Authors: Silva Emília Márcia Gomes de Souza e , de Melo Luciana Protásio , de Souza Aline Alves , de Holanda Ledycnarf Januário , Ribeiro Tatiana Souza , Valentim Ricardo Alexsandro de Medeiros , Lindquist Ana Raquel 
Published: September 2022

IntroductionCaregivers are essential during and after rehabilitation but exhibit intense physical and mental burdens due to responsibilities, resulting in stress, irritability, depression, anxiety, pain, and financial distress. Telerehabilitation offers several remote health services that improve time, engagement, and physical and mental health care access. Thus, we outlined a systematic review protocol to evaluate the impact of telerehabilitation on the burden, stress, pain, and quality of life of caregivers of patients with neurological disorders.MethodsSearches will be conducted in Ovid MEDLINE, Pubmed, Scopus, Web of Science, Embase, Cochrane Central Register of Controlled Trials, Physiotherapy Evidence Database, and PsycINFO databases. Clinical trials evaluating the burden, stress, pain, and quality of life of caregivers of patients with neurological disorders using telerehabilitation will be included without publication date or language restriction. Two reviewers will independently select studies from titles, abstracts, and reference lists. The quality of evidence and risk of bias will be assessed according to Cochrane recommendations.ResultsThis systematic review to be developed will evaluate the impact of telerehabilitation on the burden, stress, pain, and quality of life of caregivers of patients with neurological disorders.DiscussionCaregivers, especially of patients with neurological disorders, need more attention since the overload, stress, duties with other personal responsibilities, and low remuneration may impact the quality of life. Therefore, they need intervention, especially physical therapy via telehealth, which values the time of caregivers and may change their perception of health and quality of life.PROSPERO registration numberCRD42022278523.
Identification of a novel necroptosis-related classifier to predict prognosis and guide immunotherapy in breast invasive carcinoma
Authors: Zhou Qin , Xu Yan , Shen Liang , Yang Xiaochen , Wang Li 
Published: September 2022

BackgroundNecroptosis plays a crucial function in the progression of breast invasive carcinoma (BRCA). It may be triggered in cancer therapy to enhance anti-tumor immunity. However, the functions of necroptosis in tumors and its relationship with the tumor microenvironment (TME) remain largely unclear.MethodsNecroptosis-related genes (NRGs) were collated from high-quality literature reviews. A robust risk model was constructed to systematically evaluate the clinical value, functional status, effects exerted by the risk model on the TME, and the genomic variations based on the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) meta-cohorts.ResultsA risk model was constructed which comprised of six NRGs, including TNF receptor-associated factor 5 (TRAF5), Toll-like receptor 3 (TLR3), a riboflavin kinase (RFK), Fas ligand (FASLG), Fas-associated protein with death domain (FADD), and baculoviral IAP repeat-containing 3 (BIRC3). The stability and accuracy of the risk model were demonstrated for both the training and validation cohorts and its utility as an independent prognostic model for BRCA was verified. Patients in the low-risk group exhibited “hot” tumors having active immune and cell killing functions, while those in the high-risk group showed “cold” tumors having active tumor proliferation and immunosuppression. Moreover, patients in the high-risk group had a greater number of CNV events in their genome, while the somatic mutations were fewer. Furthermore, patients in the low-risk group showed high sensitivity toward immunotherapy and chemotherapy.ConclusionA reliable risk model based on NRGs to assess patient prognoses and guide clinical decision-making was constructed and validated. Our findings may contribute to the understanding of necroptosis and aid clinical management, along with precision treatment in BRCA.
Immunotherapy in advanced kidney cancer: an alternative meta-analytic method using reconstructed survival data in case of proportional hazard assumption violation
Authors: Nocera Luigi , Fallara Giuseppe , Raggi Daniele , Belladelli Federico , Robesti Daniele , Montorsi Francesco , Karakiewicz Pierre I. , Malavaud Bernard , Ploussard Guillaume , Necchi Andrea , Martini Alberto 
Published: September 2022

BackgroundWith the advent of immuno-oncology compounds in randomized trials, we observe more and more survival curves crossing. From a statistical standpoint this corresponds to violation of the proportional hazard assumption. When this occurs, the hazard ratio from the Cox regression is not reliable as an estimate. Herein, we aimed to identify the most appropriate IO-based therapy for metastatic renal cell carcinoma applying an alternative method to overcome the issue of hazard assumption violation for meta-analyses.MethodsPubmed, EMBASE, Web of Science and Scopus databases were searched. Only phase III randomized clinical trials on IO-IO (nivo-ipi) or IO-TKI combinations were included. An algorithm to obtain survival data from published Kaplan-Meier curves was used to reconstruct data on overall survival (OS), progression-free survival (PFS) and duration of response (DoR). Differences in restricted mean survival time (RMST) were used for comparisons.Resultsindividual survival data from 4,206 patients from five trials were reconciled. Patients who received nivo-ipi or IO-TKI had better OS, PFS and DoR relative to sunitinib (all p&lt;0.001). Patients who received IO-TKI had similar OS and PFS relative to nivo-ipi, with a 36-month ΔRMST of -0.55 (95% CI: -1.71-0.60; p=0.3) and -1.5 (95% CI: -2.9-0.0; p=0.051) months, respectively. Regarding DoR, patients who received nivo-ipi had longer duration of response relative to IO-TKI, with a 24-month ΔRMST of 1.5 (95% CI: 0.2-2.8; p=0.02) months.ConclusionDespite overall similar OS and PFS for patients receiving nivo-ipi and IO-TKI combinations, DoR was more favorable in patients who received nivo-ipi compared to IO-TKI. A meta-analysis based on differences in RMST is a useful alternative whenever the proportional hazard assumption is violated.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/, identifier CRD42021241421.
Oncologic outcome of multimodality treatment for sinonasal malignancies: An 18-year experience
Authors: Chen Meng-Yu , Wen Xin , Wei Yi , Chen Lin , Huang Zi-Xuan , Lu Tong , Zheng Nian-Zhen , Li Jian , Wen Wei-Ping , Wen Yi-Hui 
Published: September 2022

PurposeThe aim of this study was to retrospectively evaluate the oncologic outcomes of sinonasal malignancies (SNMs) of various histologic subtypes and investigate the impact of multimodality treatment on prognosis of SNM.MethodsSNM patients treated with curative-intent surgery from 2000 to 2018 were included. The primary outcomes were overall survival (OS). Survival was then assessed through Cox proportional hazards models.ResultsThree hundred and three patients were eligible for the analysis. The 5-year OS and event-free survival (EFS) were 61.0% (95% CI: 55.4%–67.1%) and 46.2% (95% CI: 40.4%–52.7%). The 5-year OS was the worst for malignant melanoma and the best for adenocarcinoma. Patients who received surgery had better OS than those who only received radiotherapy and/or chemotherapy. Endoscopic surgery had better OS than the open approach (p &lt; 0.05). Microscopically margin-negative resection (R0 resection) significantly benefited OS and EFS (p &lt; 0.001). No significant difference in OS was observed between patients who received macroscopic complete resection (R1 resection) followed by adjuvant therapy and patients who received R0 resection. Older age (HR = 1.02, p = 0.02), R1 resection (HR = 1.99, p = 0.02), sinonasal surgical history of more than 3 months before diagnosis (HR = 2.77, p = 0.007), and radiotherapy history (HR = 3, p = 0.006) are risk factors for worse EFS.ConclusionsCurative-intent surgery is irreplaceable in the treatment of SNM. The endoscopic approach is an effective alternative to the open approach. EFS is worse among patients with older age, R1 resection, sinonasal surgical history of more than 3 months before diagnosis, and radiotherapy history.