bluuebunny/crossref_metadata_2025_split huggingface dataset:
Water vapor fuelled brine-silk cocoon protein bio-battery for a self-lighting kettle and water-vapor panels
Authors: Jangir Himanshi , Das Mainak 
Published: June 2022

Abstract
Water vapor increases the electrical conductivity of silk cocoons, human hair, jute, and corn silk. This phenomenon is unclear. In the present study, XPS analysis of cocoons showed that water vapor reduces the surface presence of low-energy carbon species (C-C, C-H). In contrast, electron-dense, high-energy carbon species (C-N, C = C, C = O) remained unchanged, possibly enhancing surface charge hopping. While water vapor improves the conduction, the deficiency of charge carrier diminishes the effect. We increase the charge carrier by soaking the cocoon in an aqueous solution of common salt (NaCl) to amplify the current. Salt treatment followed by two-minute exposure to water vapor results in a sharp upward spike in the current (3.6 ± 1.07 mA, n = 12; mean ± SE) from the baseline (0.06 ± 0.02 mA, n = 12). After one hour, it maintains an average value of 0.39 ± 0.12, n = 12) ampere, indicating an upward shift in the baseline. Every time the cocoon charges with water vapor, the next charging cycle initiates after the cocoon dries up. Inspired by the cocoon ecology, we demonstrate an alternating 'water vapor- dry air' cycle for rapid charging and discharging of the cocoon battery. Finally, we designed a prototype of a self-lighting kettle and water-vapor panels for futuristic homes using a 'brine-silk cocoon protein bio-battery,' where moist waste heat generates electricity.
Mail-based harm reduction services are key solutions to reduce the impact of COVID-19-like crises on people who use drugs: evidence from two independent structures in France and in the USA
Authors: Torres-Leguizamon Magally , Favaro Jamie , Coello Dan , Reynaud Emmanuel G. , Néfau Thomas , Duplessy Catherine 
Published: June 2022

Abstract
Background: Harm Reduction (HR) policies for People Who Use Drugs (PWUD) have a significant positive impact on their health. Such approaches limit the spread of infections and reduce opioid overdose mortality. These policies have led to the opening of specialized centers located mainly in big cities and urbanized zones. The COVID-19 pandemic reduced access to HR centers in locations undergoing lockdown. Before the pandemic, HR services in France and in the USA were complemented by the development of remote HR programs: HaRePo (Harm Reduction by Post) for France, implemented in 2011, and NEXT Distro for the USA founded in 2017. These programs are free and specifically designed for people who have difficulties accessing HR tools and counseling in-person. PWUD can access HaRePo program by phone and/or email. NEXT Distro users can access the program through its dedicated website. The COVID-19 pandemic and the associated lockdowns have drastically impacted the HR services in both countries and many HR centers were forced to adapt their activities. Methods: Here, we analyzed how lockdowns impacted the number of new users entering the programs, the numbers of parcels sent and naloxone distributed using the records of both structures. With the ability to reach PWUD remotely, HaRePo and NEXT Distro were particularly effective at maintaining service continuity and scaling up services to meet the needs of PWUD during the COVID-19 pandemic.Results: By comparing previous year’s data average with a 95% confidence interval, we showed that the activity of both programs were significantly impacted by the pandemic. Both show an increase in new users joining the programs as well as the number of parcels sent per month. It shows that remote HR was able to partially compensate for the reduced HR activities due to COVID-19. We also observed that the distribution of naloxone per parcel tends to increase for both structures. Conclusion: By studying two independent structures in France and in the USA sharing similar objectives (remote HR), we showed that this approach can be a key solution to crises that impact classical HR structures despite various differences in operating procedures between countries.
Potential biomarker proteins for aspiration pneumonia detected by shotgun proteomics using buccal mucosa samples: A cross-sectional study
Authors: Ogura Kohei , Endo Maho , Hase Takashi , Negami Hitomi , Tsuchiya Kohsuke , Suzuki Takeshi , Ogai Kazuhiro , Sanada Hiromi , Okamoto Shigefumi , Sugama Junko 
Published: June 2022

Abstract
Background: Aspiration pneumonia (AP), which is a major cause of death in the elderly, does present with typical symptoms in the early stages of onset, thus it is difficult to detect and treat at an early stage. In this study, we identified biomarkers that are useful for the detection of AP and focused on salivary proteins, which may be collected non-invasively. This is because expectorating saliva is often difficult for elderly people, so we collected salivary proteins from the buccal mucosa.Methods: We collected samples from the buccal mucosa of six patients with AP and six control patients (no AP) in an acute-care hospital. Following protein precipitation using trichloroacetic acid and washing with acetone, the samples were analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS). We also determined the number of cytokines and chemokines in non-precipitated samples from buccal mucosa.Results: Comparative quantitative analysis of LC-MS/MS spectra revealed 179 and 181 proteins that were significantly higher and lower in abundance, respectively, in the AP group compared with the control group. We identified eight proteins (chloride intracellular channel protein 3, b-defensin 4A, protein S100-A7A, Purkinje cell protein 4-like protein 1, glutamine synthetase, protein S100-A7, cytosolic 5'-nucleotidase 3A, pyridoxal kinase) that were detected in all of the AP samples with high coverage, indicating that these proteins are promising AP biomarker candidates. In addition, the abundance of C-reactive protein (CRP) in oral samples was highly correlated with serum CRP levels, suggesting that oral CRP levels may be used as a surrogate to predict serum CRP in AP patients. A multiplex cytokine/chemokine assay revealed that MCP-1 tended to be low, indicating unresponsiveness of MCP-1 and its downstream immune pathways in AP.Conclusion: Our findings suggest that oral salivary proteins, which are obtained non-invasively, can be utilized for the detection of AP.
Clinical value and potential mechanisms of BUB1B up-regulation in nasopharyngeal carcinoma
Authors: Qin Li-Ting , Huang Si-Wei , Huang Zhi-Guang , Dang Yi-Wu , Fang Ye-Ying , He Juan , Niu Yi-Tong , Lin Cai-Xing , Wu Ji-Yun , Wei Zhu-Xin 
Published: June 2022

Abstract
Nasopharyngeal carcinoma (NPC) has insidious onset, late clinical diagnosis and high recurrence rate, which leads to poor quality of patient life. Therefore, it is necessary to further explore the pathogenesis and therapy targets of NPC. BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) was found to be up-regulated in a variety of cancers, but only one previous study showed that BUB1B was overexpressed in NPC and the sample size was small. The clinical role of BUB1B expression and its underlying mechanism in NPC require more in-depth research. Immunohistochemical samples and public RNA-seq data indicated that BUB1B protein and mRNA expression levels were up-regulated in NPC, and summary receiver operating characteristic curve indicated that BUB1B expression level had a strong ability to distinguish NPC tissues from non-NPC tissues. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes were performed and revealed that BUB1B and its related genes were mainly involved in cell cycle and DNA replication. Protein- Protein Interaction were built to interpret the BUB1B molecular mechanism. Histone deacetylase 2 (HDAC2) could be the upstream transcription factor of BUB1B, which was verified by Chromatin Immunoprecipitation Sequencing samples. In summary, BUB1B was highly expressed in NPC, and the predicted BUB1B upstream transcription factor HDAC2 may affect cell cycle by regulating BUB1B to promote cancer progression.
Secondary Budd-Chiari syndrome occurred after adjuvant radiotherapy for perihilar cholangiocarcinoma: A case report
Authors: Miura Yuya , Ashida Ryo , Saiga Atsushi , Sugiura Teiichi , Ohgi Katsuhisa , Yamada Mihoko , Otsuka Shimpei , Aramaki Takeshi , Sato Rui , Uesaka Katsuhiko 
Published: June 2022

Abstract
Background: Budd–Chiari syndrome (BCS) is a rare vascular disorder of the liver, and acute and secondary BCS is even rarer. Case presentation: A 62-year-old man with perihilar cholangiocarcinoma of Bismuth type IIIa underwent right hemi-hepatectomy with caudate lobectomy and pancreatoduodenectomy. Adjuvant chemoradiotherapy was performed due to a positive hepatic ductal margin. Subsequently, the disease passed without recurrence. The patient visited for acute onset abdominal pain at the 32nd postoperative month. Multidetector-row computed tomography (MDCT) showed stenosis of the left hepatic vein (LHV) root, which was the irradiated field, and thrombotic occlusion of the LHV. The patient was diagnosed with acute BCS caused by adjuvant radiotherapy. Although anticoagulation therapy was performed, the patient complained of sudden upper abdominal pain again. MDCT showed an enlarged LHV thrombus and hepatomegaly. The patient was diagnosed with exacerbated acute BCS, and stenting for the stenotic LHV root was performed with a bare stent. Although stenting for the LHV root was very effective, restenosis occurred twice due to thrombus in the existing stent, so re-stenting was performed twice. The subsequent clinical course was acceptable without recurrence or restenosis of the LHV root as of six months after the last stenting using a stent graft.Conclusion: Although no case of BCS caused by radiotherapy has yet been reported, the present case showed that late side effect of radiotherapy can cause hepatic vein stenosis and secondary BCS.
The Role of Green Gram Plant Volatile Blends in the Behavior of Arctiid Moth, Spilosoma Obliqua
Authors: Mobarak Syed Husne , Koner Anamika , Debnath Rahul , Barik Anandamay 
Published: June 2022

Abstract
Larvae of Spilosoma obliqua Walker (Lepidoptera: Arctiidae) feed on the leaves and flowers of green gram [Vigna radiata (L.) Wilczek] and reduce seed yield. Therefore, it is interesting to observe whether undamaged (UD) and insect-damaged (ID, plants after feeding by S. obliqua larvae) plants cause attraction of conspecific adult moths. Females showed attraction towards volatile blends of UD, ID and mechanically-damaged (MD) plants of three green gram cultivars [PDM 54 (PDM), Pusa Baisakhi (PUSA) and Samrat (SAM)] in Y-tube olfactometer bioassays. Females were more attracted towards volatile blends of ID plants of a particular cultivar than UD plants of the same cultivar. Total amount of volatiles was higher in ID plants than UD plants. Some herbivore-induced plant volatiles − 3Z-hexenal, 1-hexanol, 3Z-hexenyl acetate, 2-octanol and ocimene were attractive to the insect. Females were attracted towards three synthetic blends (µg in 25 µl CH2Cl2) comprised − 2.97 µg 3Z-hexenal, 4.92 µg 1-hexanol, 5.56 µg benzaldehyde, 9.76 µg 3Z-hexenyl acetate, 7.27 µg 2-octanol, 7.97 µg ocimene and 5.71 µg acetophenone resembling PDM, 4.68 µg 3Z-hexenal, 8.38 µg benzaldehyde, 7.66 µg 3Z-hexenyl acetate, 5.96 µg ocimene and 7.60 µg acetophenone resembling PUSA, and 3.39 µg 3Z-hexenal, 6.29 µg benzaldehyde, 8.74 µg 3Z-hexenyl acetate, 6.17 µg ocimene and 3.69 µg acetophenone resembling SAM in olfactometer and wind tunnel bioassays. Females could not distinguish between these three synthetic blends in olfactometer and wind tunnel bioassays, suggesting that these three synthetic blends could be used as lures in developing baited traps in integrated pest management strategies.
From marginal zone lymphoma to aggressive diffuse large B-cell lymphoma: a whole-exome and clinicopathological characteristics analysis of transformed lymphoma
Authors: Zhao Xia , Bian Haiyan , Hao Fengyun , Shao Shihong , Wu Chuanhong , Zhang Qian , Zhao Xiangzhong , Wu Mingxuan , Li Zhiqiang , Gao Chengwen 
Published: June 2022

Abstract
Transformed lymphoma occurs when indolent lymphoma transforms into more aggressive lymphoma and are associated with poor prognosis. In this study, we analyzed the immunophenotypes, prognostic factors, and outcomes of 35 patients with transformed lymphoma from among 306 marginal zone lymphoma (MZL), 544 follicular lymphoma (FL), and 871 chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL) cases. In addition, we performed whole-exome sequencing study of 7 transformed MZL (tMZL) cases. Our results demonstrate that the median time from indolent lymphoma diagnosis to transformed DLBCL was 35 months (range, 14–53 months). The 5-year overall survival (OS) and progression-free survival (PFS) rates after histological transformation (HT) were 50% and 26%, respectively. Kaplan-Meier survival analysis revealed that asynchronous HT and transformed CLL/SLL (tCLL/SLL) were significant adverse prognostic factors for OS after DLBCL HT. Our study found that TNFAIP3 was the most frequently mutated gene in tMZL samples. We identified mutations involvement in chromatin remodeling (CREBBP and EP300) and regulators of NF-κB signaling (TNFAIP3, BCL10, MYD88, CD79B, and CARD11) were affected in tMZL. Whole-exome sequencing and copy-number analysis revealed that tMZL derives from the divergent evolution of an ancestral common progenitor clone (CPC). Collectively, this study provides clinicopathological characteristics of three common types of transformed lymphomas and the genetic profile of tMZL with diagnostic and therapeutic implications.
The origin for the increased Curie temperature of B20-MnSi films
Authors: Li Zichao , Yuan Ye , Begeza Viktor , Rebohle Lars , Helm Manfred , Nielsch Kornelius , Prucnal Slawomir , Zhou Shengqiang 
Published: June 2022

Abstract
B20-type MnSi is the prototype magnetic skyrmion material. Thin films of MnSi show a higher Curie temperature than their bulk counterpart. However, it is not yet clear what mechanism leads to the increase of the Curie temperature. In this work, we grow MnSi films on Si(100) and Si(111) substrates with a broad variation in their structures. By controlling the Mn thickness and annealing parameters, the pure MnSi phase of polycrystalline and textured nature as well as the mixed phase of MnSi and MnSi1.7 are obtained. Surprisingly, all these MnSi films show an increased Curie temperature of up to around 43 K. The Curie temperature is likely independent of the structural parameters within our accessibility including the film thickness above a threshold, strain, cell volume and the mixture with MnSi1.7. However, a pronounced phonon softening is observed for all samples, which can tentatively be attributed to slight Mn excess from stoichiometry, leading to the increased Curie temperature.
C-type lectin domain family 4 member G(CLEC4G) is a negative marker for CD34 in the evolution of liver pathogenesis
Authors: Chen Xiang , Jiang Zui-ming , Gu Min , Feng Bin , Xie Zhi-qin , Huang Mei-yuan , Chen Zhu , Tang Man-ling 
Published: June 2022

Abstract
Purpose: This study aimed to explore the expression of the C-type lectin domain family 4 member G (CLEC4G) gene in the liver sinusoidal endothelial cells (LSECs) during liver pathogenesis, and to evaluate its correlation with CD34 and clinical significance in hepatocellular carcinoma patients. Methods: We conducted bioinformatics analysis of the differential expression of CLEC4G in in various human organs, carcinomatous and adjacent tissues. Then, the differential expression in carcinomatous and adjacent tissues was verified by real-time quantitative reverse transcription PCR (qRT-PCR) and immunohistochemical (IHC) analyses in clinical HCC samples. The expression of CLEC4G in normal liver tissues and cirrhotic tissues was further detected. Besides, mRNA and protein expression levels of CD34 in HCC samples were detected via qRT-PCR and IHC, respectively. ELISA was applied to detect serum levels of CLEC4G in healthy controls, liver fibrosis and HCC patients. The correlation between the CLEC4G and CD34 expression was analyzed using the Spearman test, whereas the Chi-square test was applied to analyze the correlation between CLEC4G and clinicopathological characteristics of HCC patients. Furthermore, the Kaplan-Meier method was used to estimate the relationship between CLEC4G serum levels and survival. Results: The expressions of mRNA and protein levels of CLEC4G were higher in normal liver tissues, moderately expressed in cirrhotic and para-cancerous tissues (P<0.001), and lowest in HCC tissues (P<0.001). We also found high CD34 expression in late tumors, which was negatively correlated with CLEC4G at both mRNA and protein levels (R=-0.830, P<0.001; R=-0.817, P<0.001, respectively). Compared to the healthy controls, the CLEC4G levels in liver fibrosis patients, and HCC patients were gradually lower (P<0.001). Furthermore, CLEC4G was highly associated with tumor size (P=0.023), tumor stage (P=0.046), and vascular metastasis (P=0.008; Table 1) in HCC patients. Notably, HCC patients expressing low CLEC4G levels were characterized by a shorter survival time (P=0.032). Conclusions: The low expression of CLEC4G is potentially correlated with LSEC capillarization and the appearance of micro-vessels. Such a phenomenon may exert an endogenous protective effect against the progression of liver disease and serve as a reliable diagnostic marker for hepatocellular carcinoma.
Tansmembrane TNF-α as a novel biomarker for the diagnosis of cytokine storm in a mouse model of multiple organ failure
Authors: yang peng , Zeng Yimin , Yang Fang , peng xin , Hu Yongsheng , Tan Xuhong , Zhang Ruping 
Published: June 2022

Abstract
A cytokine storm(CS) is an out-of-control inflammatory response closely associated with the progression of diseases, such as multiple organ failure(MOF), severe sepsis, and severe or critical COVID-19. However, there is currently a lack of reliable diagnostic markers to distinguish CS from normal inflammatory responses. Tumor necrosis factor-α (TNF-α) includes transmembrane TNF-α (tmTNF-α) and secreted TNF-α (sTNF-α). The MOF mouse model in this study showed that the tmTNF-α expression changes in the neutrophils differed from the serum TNF-α and serum IL-4, IL-6, IL-10, and IL-18 and it was the tmTNF-α, instead of serum TNF-α, IL-4, IL-6, IL-10, and IL-18, that reflected the liver and kidney tissue damage and increased with the aggravation of these injuries. Analysis of the ROC results showed that tmTNF-α effectively distinguished between inflammatory response and CS and efficiently differentiated between surviving and dead mice. It also significantly improved the diagnostic value of the traditional CRP marker for CS. These results indicated that tmTNF-α expressed in the neutrophil could be used to diagnose CS in MOF mice, providing an experimental basis to further develop tmTNF-α for diagnosing CS patients.
Rapid Malignant Transformation of Adrenal Cortical Adenoma: A Case Report and Review of the Literature
Authors: Cao Zhenzhen , Deng Qian , Cui Mengying , Ge Kele , Wu Chen , Wu Jun , Wu Changping 
Published: June 2022

Abstract
With the development of science and technology, adrenal incidentaloma (AI) is more and more recognized, and adrenal cortical carcinoma (ACC) is an extremely rare and aggressive cancer of the adrenal gland. According to the report, the risk of malignancy was 0.1% in ACC species with benign initial Computed tomography (CT) features. And, in most cases, a transition period of seven to 10 years is required. In 2018, a patient with an AI was admitted to our hospital. The pathological diagnosis of the operative specimen was adrenal cortical adenoma (ACA). Two years after the surgery, the patient was admitted to our hospital again with abdominal pain and neck pain. CT scan revealed a large retroperitoneal mass with intratumor hemorrhage. Subsequent positron emission tomography (PET) revealed multiple metastases to the liver and bone. The pathological diagnosis of liver and retroperitoneal metastatic lesions was an adrenal cortical tumor (ACT). Given the biological behavior and imaging results of the malignant invasion of the patient's tumor, we finally diagnosed ACC. In this case report, we discuss the cause and possibility of the patient's rapid transition from ACA to ACC in less than two years and review the literature.
Electronic Cigarette Portrayal in Indonesia: A Content Analysis of News Media
Authors: Bigwanto Mouhamad , Arumsari Imas , Fauzi Ridhwan 
Published: June 2022

Abstract
Background: The news media play an essential role in shaping public opinion. Analyzing a specific issue reported in the news media provide insight and considerations for a government to form a policy. The study aimed to assess the portrayal of the electronic cigarette issue in Indonesian news media, including the variety of arguments being presented and the actors behind it.Methods: We used a paid service database from eBdesk to collect the Indonesian news articles from 2020 to 2021 using the keywords; 'rokok elektronik', 'rokok elektrik', 'e-rokok', 'vape' and 'vaping'. Content analysis of 551 full-text news articles was conducted to identify the concepts, topics, and frames of quoted arguments and to examine whether any frames were presented on the different types of organizations and the origin of actors. Results: News articles related to e-cigarettes were mostly published in the national newspaper and in the non-health section at the newspaper desk. A total of 891 arguments from 393 persons representing 212 institutions were identified. Twenty-eight concepts were further categorized into 12 topics. Health impact was the most frequently reported topic, followed by regulation, tax/price issues, and e-cigarettes as smoking cessation tools. Overall, the articles and arguments with positive tones toward e-cigarettes outnumbered negative articles and arguments. The industry, university and research-based institutions were the most involved type of organizations in the news articles report. There were more equal frames toward e-cigarettes among the international actors and government, even though the frame within the non-health government sector was reported to be more positive towards e-cigarettes.Conclusions: Although health impact is the most reported topic, the actors involved in delivering arguments were mainly from the non-health sector, including where the news articles were published on the newspaper desk. The E-cigarettes issue in Indonesia was mainly played by the non-health sector, which is more inclined toward economic interest than health.