bluuebunny/crossref_metadata_2025_split huggingface dataset:
Safe Provision of Systemic Anti-Cancer Treatment for Urological Cancer Patients During COVID-19: A Tertiary Centre Experience in the First Wave of COVID-19
Authors: So Alfred Chung Pui , Karampera Christina , Khan Muhammad , Russell Beth , Moss Charlotte , Monroy-Iglesias Maria J , Thillai Kiruthikah , Josephs Debra Hannah , Pintus Elias , Rudman Sarah , Hemelrijck Mieke , Dolly Saoirse , Enting Deborah 
Published: January 2022

Abstract
Background

Safe provision of systemic anti-cancer treatment (SACT) during the COVID-19 pandemic remains an ongoing concern amongst clinicians.
Methods

Retrospective analysis on uro-oncology patients who continued or started SACT between 1st March and 31st May 2020 during the pandemic (with 2019 as a comparator).
Results

441 patients received SACT in 2020 (292 prostate, 101 renal, 38 urothelial, 10 testicular) compared to 518 patients in 2019 (340 prostate, 121 renal, 42 urothelial, 15 testicular). In 2020, there were 75.00% fewer patients with stage 3 cancers receiving SACT (p<0.0001) and 94.44% fewer patients receiving radical treatment (p=0.0019). The number of patients started on a new line of SACT was similar between both years (118 in 2019 vs 102 in 2020; p=N.S) but with 53.45% fewer patients started on chemotherapy in 2020 (p=0.00067). Overall, 5 patients tested positive for COVID-19 (one asymptomatic, two moderate pneumonitis, one severe pneumonitis). Compared to 2019, 30-day mortality was similar (1.69% in 2019 vs 0.98% in 2020; p=N.S) whereas the 6-month mortality was lower (9.32% in 2019 vs 1.96% in 2020; p=0.023) in 2020.
Conclusion

This single-centre study demonstrated that uro-oncology patients can safely receive SACT during COVID-19 pandemic with a low incidence of infection and mortality.
High Pressure and Polaron Effects on Optical Properties of GaN Wedge-Shaped Quantum Dot
Authors: Sayari A. , Servatkhah M. , Pourmand R. 
Published: January 2022

Abstract
In the paper, the optical properties of GaN wedge-shaped quantum dot (WSQD) under influence both pressure and electron-phonon (e-p) interaction have been investigated. To this end, the energy difference and dipole matrix elements are evaluated by considering the effects of e-p and pressure. Then, the refractive index changes (RICs) and absorption coefficients (ACs) are evaluated. It is deduced that the energy difference and dipole matrix elements reduce by considering the e-p and pressure effects. It is resulted that the pressure enhances the e-p effect on the energy difference. The ACs and RICs move to lower energies by considering the e-p and pressure effects. The RICs and ACs are raised in the presence of e-p effect whereas they are reduced with considering the pressure. With considering the e-p and pressure effects on total ACs, it is seen that the peak of AC splits into two peaks.
ERBB3 Methylation and Immune Infiltration in Tumor Microenvironment of Cervical Cancer
Authors: Yang Xiaoyue , Chen Ying , Li Mei , Zhu Weipei 
Published: January 2022

Abstract
ERBB3, a member of the ERBB family of receptor tyrosine kinases, plays an important role in cancer, despite its lack of intrinsic Carcinogenic mechanism of CESC. Research on bioinformatics methods through multi-omics, this work proves that ERBB3 gene mutation, methylation modification have extensive regulatory mechanisms on the CESC microenvironment. We found that ERBB3 is involved in carcinogenesis of cervical cancer and is not associated with its prognosis. The carcinogenic mechanism is mainly related to the suppression of the immune system between TILS and the methylation of the RNA level. Our study indicated ERBB3 is more likely to be a carcinogenic factor than a key prognostic factor for cervical cancer. Methylation of ERBB3 may work as a chekpoint immunotherapy target in CESC, DNA methylation modification of the 4480 base pair downstream of ERBB3 transcription initiation site was the highest.
Association of HMGB1, S100A12 and IL-17A Expression with Immunoglobulin-Resistant in Kawasaki Disease and the Comparisons Between Different Adjunctive Therapeutic Approaches
Authors: Zhou Yang , Pan Liu , Yang You-jun , Li Shi-yu , Yin Wei , Liu Fan , ding yan 
Published: January 2022

Abstract
Objective: The DAMPs such as HMGB1, S100A12 and IL-17A have been reported to predict poor response to IVIG. The aim of this study was to analyze the role of HMGB1,S100A12 and IL-17A in the detection of inflammation in KD patients with IVIG-resistant, and to investigate the value of different adjunctive therapy.Method: This study enrolled 126 patients diagnosed with KD, as well as age-matched 16 febrile control subjects. The demographic or clinical data, laboratory parameter and blood sample were collected. Various laboratory parameters as predictive factors for IVIG-resistant were calculated. And the serum levels of IL-17A and mRNA expression levels of HMGB1 and S100A12 were tested in all patients. For patients with acute KD in IVIG-resistant, we studied the levels of laboratory variables when using of IVIG retreatment, methylprednisolone, infliximab for children patients. Result: The variance of laboratory parameters between the febrile control group and KD group were analyzed. Regarding laboratory parameters, KD individuals were found to have lower levels of L%, PA, CD4+, CD8+ and higher levels of WBC, N%, CRP, ESR, NT-proBNP, ALT, CD4+/CD8+ (P<0.05 or P<0.01). For KD group, the 53 IVIG-resistant patients had significantly higher levels of blood S100A12, HMGB1, serum IL-17A levels And N%, CRP, NT-pro BNP, TBIL, ALT, AST and lower levels of L%, PLT (P<0.05 or P<0.01) in comparison to the IVIG-responsive patients. For patients with acute KD in IVIG-resistant, after initial IVIG-treatment, the adjunctive therapy of IVIG, methyl prednisolone or infliximab were used, the inflammatory symptoms and laboratory inflammatory markers were improved when treated with those drugs. Conclusion: IVIG-resistant was associated with higher levels of HMGB1, S100A12, IL-17A, CRP, NT-pro BNP, TBIL, ALT, AST and lower levels of L%, PLT before IVIG, especially when combined, were useful predictors for IVIG-resistant in KD. In addition, the adjunctive therapy of methylprednisolone and infliximab showed more effective in relief clinical symptoms than IVIG retreatment.
Multi-Omics Study of The Salivary Modulation of The Rumen Microbiome
Authors: Palma-Hidalgo Juan Manuel , Belanche Alejandro , Jiménez Elisabeth , Martín-García A. Ignacio , Newbold Charles J. , Denman Stuart E. , Yáñez-Ruiz David R. 
Published: January 2022

Abstract
Ruminants are able to produce large quantities of saliva which enter into the rumen. Although previous research has indicated that salivary immunoglobulins can partially modulate the rumen microbial activity, the role of the salivary components other than ions on the rumen microbial ecosystem has not been thoroughly investigated in ruminants. A total of 16 semi-continuous in vitro cultures were used to incubate rumen fluid from 4 donor goats inoculated with autoclaved saliva (AUT) as negative control, saliva from the same rumen fluid donor (OWN) as positive control, and either GOAT or SHEEP saliva as experimental interventions. Fermentation was monitored throughout the 7 days of incubation and the prokaryotic communities and metabolome were analysed at day 7 of incubation. Characterization of the salivas used prior to incubation showed a high degree of individual variability in terms of the salivary metabolites and proteins, including immunoglobulins. The prokaryotic community composition in AUT incubators was the most divergent across treatments, suggesting a modulatory effect of active salivary components, which were not affected in the other treatments (OWN, GOAT and SHEEP). The differences across treatments in microbial diversity were mostly caused by a greater abundance of Proteobacteria and Rikenellacea and lower of Prevotellaceae, a key rumen bacterium with greater abundance in GOAT and SHEEP treatments. These results suggest that specific salivary components contribute to host-associated role in selecting the rumen commensal microbiota and its activity.
Nanocluster Mediated Photothermia Improves Eradication Efficiency and Antibiotic Sensitivity of Helicobacter Pylori
Authors: Meng Fansen , Tao Hongjin , Mi Yan , Yang Tianyu , Wang Xuanping , Go Yuyo , Lin Yunjuan , Wang Gangshi 
Published: January 2022

Abstract
Background: Helicobacter pylori (H. pylori) eradication plays a crucial role in gastric cancer prevention, but the antimicrobial resistance of H. pylori is obstructing this elimination process. In this study, we developed nanoclusters (NCs) from Zn0.3Fe2.7O4 nanoparticles using a poly(ethylene glycol)-b-poly(ε-caprolactone)-based nanocarrier as an innovative antibiotic-independent H. pylori management.Results: The nanocluster showed minimal toxicity and maximal biocompatibility. With a low concentration (50 µg/mL) of NCs under a short time period (～2 min) of near-infrared (808nm) irradiation, we kept the culture medium temperature to 41 °C for 20 minutes with continuous irradiation. The heated NCs exhibited efficient photothermal effects and resulted in an excellent inhibition of H. pylori growth, adhesion ability and cell vacuolization ability in in vitro investigation. Transmission electron microscopy showed a dramatic morphologic change after NCs photothermia on H. pylori, including cell wall and membrane rupture, as well as ribosome damage. Besides, levofloxacin and clarithromycin resistance were improved after photothermal treatment in H. pylori NCTC 11637 and/or clinical strains, however metronidazole resistance was unchanged. We also discovered a significant decrease in the biofilm formation of H. pylori under the NCs-based photothermal application, while efflux pump function was unchanged.Conclusions: Based on this novel NCs-based photothermal approach, we were able to demonstrate in vitro a significant inhibition of both H. pylori growth and molecular toxicity, and its improvement in antibiotic resistance alone with the eradication of H. pylori biofilms previously believed to be resistant to conventional antibiotics.
Comparison of Low-Cost Methods for Soil Water Holding Capacity
Authors: Govindasamy Prabhu , Mahawer Sonu Kumar , Mowrer Jake , Bagavathiannan Muthukumar , Prasad Mahendra , Ramakrishnan Srinivasan , Halli Hanamant M , Kumar Sunil 
Published: January 2022

Abstract
Purpose: The use of cost-effective methods for measurement of WHC is common in underdeveloped and developing countries, but the accuracy of these cost-effective methods compared to the sophisticated and more expensive alternatives is unclear. Methods: To compare different WHC measurement methods, 30 random samples of clay loam and sandy clay loam soils of Jhansi, India were used. The methods compared here were: FAO in-situ method (FAO), Keen Raczkowski box method (KM), funnel method (FM), column method (CM) and pressure plate method (PPA). Results: For WHC measurements the PPA results were comparable to KM and FM methods for sandy clay loam, and KM and FAO methods for clay loam. Conclusion: Therefore, until a reliable method that matches the results of sophisticated analytical methods of soil water measurement is available, different inexpensive analytical methods can be used, but they must be chosen with caution. The findings from this study will facilitate appropriate selection of a suitable method.
Spatiotemporal Characteristics of Meteorological Drought During the Past Half Century in Different Climates Over Iran
Authors: Ghaleni Mehdi Mohammadi , Sharafi Saeed , Hosseini-Moghari Seyed-Mohammad , Helali Jalil , Oskouei Ebrahim Asadi 
Published: January 2022

Abstract
The present study compares the main characteristics (intensity, duration, and frequency) of meteorological drought events in the four climates (Hyperarid, Arid, Semiarid, and Humid) of Iran. For this purpose, three drought indices, including Standardized Precipitation Index (SPI), Reconnaissance Drought Index (RDI), and Standardized Precipitation Evapotranspiration Index (SPEI), were employed at the timescales of 1-, 3-, 6-, 9- and 12-months. These indices were compared by utilizing long-term data of 41 synoptic meteorological stations for the recent half century, 1969–2019. The long-term analysis of drought indices indicates that the duration and intensity of drought events have temporally risen after the 1998–99 period. Iran has experienced the longest duration (40 months) of extreme drought during Dec 98–Mar 02 and Jan 18–Mar 18, respectively. Spatial patterns demonstrate that drought intensity uniformly increased in SPI1 to SPI12, and SPEI3 to SPEI12, from humid and semiarid to arid and hyperarid regions. The average drought duration in studied stations for SPI, SPEI and RDI indices equaled 9, 12, and 9 months, respectively. In addition, mean drought frequencies are calculated at 14, 17, and 13 percent for SPI, SPEI and RDI indices, respectively. Generally, SPEI compared to SPI and RDI shows greater duration and frequency of drought events, particularly in arid and hyperarid regions. The research shows the crucial role of climatic variables in detecting drought characteristics and the importance of selecting appropriate drought indices in various climates.
SLGT2 Inhibitor Rescues Myelopoiesis in G6PC3 Deficiency
Authors: Hiwarkar Prashant , Bargir Umair , Pandrowala Ambreen , Bodhanwala Minnie , Thakker Naresh , Taur Prasad , Madkaikar Manisha , Desai Mukesh 
Published: January 2022

Abstract
The energy metabolism of myeloid cells depends primarily on glycolysis. 1,5-anhydroglucitol (1,5AG), a natural monosaccharide is erroneously phosphorylated by glucose-phosphorylating enzymes to produce 1,5-anhydroglucitol-6-phosphate (1,5AG6P), a powerful inhibitor of hexokinases. The endoplasmic reticulum transporter (SLC37A4/G6PT) and the phosphatase G6PC3 co-operate to dephosphorylate 1,5AG6P. Failure to eliminate 1,5AG6P is the mechanism of neutrophil dysfunction and death in G6PC3-deficient mice. SLGT2-inhibitor reduces 1,5AG level in the blood and restores the neutrophil count in G6PC3-deficient mice. In the investigator-initiated study, a 30 year-old G6PC3-deficient woman with recurrent infections, distressing gastrointestinal symptoms and multi-lineage cytopenia was treated with an SLGT2-inhibitor. A significant increase in all the hematopoietic cell lineages and substantial improvement in the quality of life was observed.
Clinical Performance of Friend Leukemia Virus Integration 1 Methylation as a Biomarker for Colorectal Carcinoma
Authors: Wang Jing , Xue Cai-Lin , He Yuan 
Published: January 2022

Abstract
Objectives

Accumulating evidence supports the reliability of molecular biomarkers and cancer, the current situation is lacking. This present study was aimed to investigate clinical performance of friend leukemia virus integration 1 (FLI1) methylation as a biomarker for colorectal cancer (CRC).
Methods

The datasets of UALCAN, GEPIA, cBioPortal, STRING, TIMER2.0, GEO, and KOBAS were utilized in this present study. In order to testify the methylation function of FLI1 in CRC, we studied 6 CRC cell lines and 20 pairs of tumor tissues. The transcriptional levels of FLI1 were tested by reverse transcription PCR quantitatively and western blot. Cell viability, transwell assays and plate clone assay were utilized to assess the cell function.
Results

FLI1 was up-regulated in the GBM, KIRC, LAML, etc. Meanwhile, it was down-regulated in BLCA, BRCA, CESC, etc (p<0.05). The CPTAC dataset showed higher total protein in the primary tissues of KIRC, lower protein in the BRCA, OV, LUAD, UCEC than normal tissues(p<0.05). Highly expressed FLI1 was linked to poor prognosis of overall survival (OS) in LGG, UVM (p<0.05). Low expression of FLI1 was associated with short OS in KIRC, LUAD, SKCM (p<0.05). Compared with normal tissues, the methylation level of FLI1 was increased in BRCA, CESC, CHOL, COAD, etc (p<0.05). In the contrary, it was decreased in KIRP, PCPG obviously (p<0.05). Moreover, it showed a reduced phosphorylation for selected probes (BRCA: NP_002008.2:S39, NP_002008.2:S3241; OV: NP_002008.2:S39; LUAD: NP_002008.2:S79, NP_002008.2:S3241; all p<0.05). Meanwhile, it showed an enhanced phosphorylation level of FLI1 in KIRC for selected probes (NP_002008.2:S241, p<0.05). Besides, a statistical negative correlation was found between FLI1 and Treg cells, neutrophils, monocytes, macrophages, NK cells, cancer-associated fibroblasts, and common immune checkpoint gene levels (p<0.05). Besides, in vivo experience showed that 5-Aza-2’-deoxycytidine diminished FLI1 methylation level and restored transcriptional levels (p>0.05) in CRC. In vitro experiments demonstrated that the proliferation, colony formation, invasion and migration of CRC cells were inhibited by FLI1 overexpression through FMNL1 (p<0.05).
Conclusions

This present study offers a comprehensive understanding of FLI1 in carcinomas with oncogenesis and immunotherapeutic implications. Moreover, FLI1 reduced the proliferation, colony formation, invasion and migration of CRC by FMNL1.
An Unusual Presentation of Cutaneous Alternariosis with Bullous Lesions in A Patient with Diabetes Mellitus
Authors: wang xiaopo , Ling Shiqi , Liang Guanzhao , Sun Jianfang 
Published: January 2022

Abstract
Diabetes mellitus considered to represent an immunodeficient state significantly predisposes patients to all types of opportunistic invasive fungal infection. However, very few cases of cutaneous alternariosis associated with diabetes have been reported previously. Herein, we describe a rare case of cutaneous alternariosis in an elderly diabetic patient with antecedent local trauma.
Synthetic MRI for Stroke: A Qualitative and Quantitative Pilot Study
Authors: André Joachim , Barrit Sami , Tchofo Patrice Jissendi 
Published: January 2022

Abstract
PurposeSynthetic MR provides quantitative multiparametric data about tissue properties in a fast single-acquisition. We compared synthetic and conventional image quality and investigated synthetic relaxometry of acute and chronic ischemic lesions to support its interest in stroke imaging. MethodsFor this pilot study, we prospectively acquired synthetic and conventional brain MR of 43 consecutive adult patients with suspected stroke. We studied a total of 136 lesions, of which 46 DWI-positive with restricted ADC (DWI+/rADC), 90 white matter T2/FLAIR hyperintensities (WMH), and 430 normal brain regions (NBR). We assessed image quality for lesion definition according to a 3-level score by two readers of different experiences. We compared relaxometry of lesions and regions of interest.Results Synthetic images were superior to their paired conventional images for lesion definition except for sFLAIR (sT1 or sPSIR vs. cT1 and sT2 vs. cT2 for DWI+/rADC and WMH definition; p-values <.001) with substantial to almost perfect inter-rater reliability (κ ranging from 0.711 to 0.932, p-values <.001). We found significant differences in relaxometry between lesions and NBR and between acute and chronic lesions (T1, T2, and PD of DWI+/rADC or WMH vs. mirror NBR; p-values <.001; T1 and PD of DWI+/rADC vs. WMH; p-values of 0.034 and 0.008).Conclusion Synthetic MR may contribute to stroke imaging by fast acquiring consistent relaxometry data and accessible derived images of interest for the study of ischemic lesions.
Performance of PROPELLER FSE T2WI in Reducing Metal Artifacts for Patients with Various Material Porcelain Fused To Metal Crown: A Preliminary Study
Authors: Li Wenjin , Shi Jing , Bian Wenjin , Li Jianting , Chen Xiaoqing , Feng Juan , Yu Jiali , Wang Jun , Niu Jinliang 
Published: January 2022

Abstract
This study aimed to compare MRI quality between common fast spin echo T2 weighted imaging (FSE T2WI) with periodically rotated overlapping parallel lines with enhanced reconstruction (PROPELLER) FSE T2WI for patients with various porcelain fused to metal (PFM) crown and analyze the value of PROPELLER technique in reducing metal artifacts. Common FSE T2WI and PROPELLER FSE T2WI sequences for axial imaging of head were applied in participants with different PFM crowns: cobalt-chromium (Co-Cr) alloy, pure titanium (Ti), gold-palladium (Au-Pd) alloy. Two radiologists evaluated overall image quality of section in PFM using a 5-point scale qualitatively and measured the maximum artifact area and artifact signal-to-noise ratio (SNR) quantitatively. The metal crown with the least artifacts and the optimum image quality shown in common FSE T2WI and PROPELLER FSE T2WI were in Au–Pd alloy, Ti, and Co–Cr alloy order. PROPELLER FSE T2WI was superior to common FSE T2WI in improving image quality and reducing artifact area for Co-Cr alloy (17.0±0.2% smaller artifact area, p＜0.001) and Ti (11.6± 0.7 % smaller artifact area, p=0.005), but had similar performance compared to FSE T2WI for Au-Pd alloy. For all PFMs, PROPELLER FSE T2WI significantly reduced the signal-to-noise ratio (SNR) of artifact (393.57±89.75 VS. 214.05±70.45, p < 0.001) when compared to common FSE T2WI.Therefore, the different PFM crown generate varying degrees of metal artifacts in MRI, and the PROPELLER can effectively reduce metal artifacts especially in the PFM crown of Co-Cr alloy.
Use of PiggyBac Transposon System Constructed Murine Breast Cancer Model For Reporter Gene Imaging And Characterization of Metastatic Tumor Cells
Authors: Chen Ying-Ling , Hou Kuei-Yuan , Lin Min-Ying , Lin Yu-Chuan , Chuang Hui-Yen , Lee Yi-Jang 
Published: January 2022

Abstract
The piggyBac transposon system is known to non-viral integrate exogenous genes to chromosomes of mammalian cells. For reporter gene imaging, this transposon system is believed to efficiently establish xenograft tumor model with low immunogenicity. Because tumor cells usually exhibit genomic instability, it is important to investigate if piggyBac mediated transduction of reporter genes would change tumor characteristics. In this study, reporter gene imaging mediated by the piggyBac transposon system was exploited to track the growth and dissemination of 4T1 triple-negative murine breast cancer cells in vivo, followed by ex vivo analysis of the metastatic cells expressing reporter genes. We demonstrated that several cell properties, including proliferation rate, invasion and migration rate, and mammosphere formation ability of 4T1 cells were not influenced by piggyBac transposon system. Further, we isolated the liver metastatic cells, named 4T1-3R_L cells for further analysis. Compared to parental 4T1 cells, 4T1-3R_L cells exhibited several cancer stem cells (CSC) related characteristics, including significant mammosphere formation ability, resistance to doxorubicin, high tumorigenicity potential in Balb/C mice and expression of CD44 CSC marker. We also found that 4T1-3R_L cells exhibited stronger migrated and invasive abilities, by wound healing assay and in vitro invasion assay, respectively. The cell adhesive ability of 4T1-3R_L cells was also lower than that of 4T1 cells. The microarray assay showed that several epithelial-mesenchymal transition (EMT) promoting markers, including vimentin, N-cadherin, Twist1, and Snail were up-regulated, and anti-EMT marker E-cadherin was down-regulated in 4T1-3R_L cells. Current data suggest that the piggyBac transposon system is a reliable and biocompatible tool to engineer cancer cells for tacking and characterizing tumor development in vivo and in vitro.