bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crystal structure of Sphingobacterium multivorum serine palmitoyltransferase complexed with tris(hydroxymethyl)aminomethane
Authors: Ikushiro Hiroko , Takahashi Aya , Murakami Taiki , Katayama Asuka , Sawai Taiki , Goto Haruna , Miyahara Ikuko , Kamiya Nobuo , Yano Takato 
Published: November 2022

Serine palmitoyltransferase (SPT) catalyses the first reaction in sphingolipid biosynthesis: the decarboxylative condensation of L-serine (L-Ser) and palmitoyl-CoA to form 3-ketodihydrosphingosine. SPT from Sphingobacterium multivorum has been isolated and its crystal structure in complex with L-Ser has been determined at 2.3 Å resolution (PDB entry 3a2b). However, the quality of the crystal was not good enough to judge the conformation of the cofactor molecule and the orientations of the side chains of the amino-acid residues in the enzyme active site. The crystal quality was improved by revision of the purification procedure and by optimization of both the crystallization procedure and the post-crystallization treatment conditions. Here, the crystal structure of SPT complexed with tris(hydroxymethyl)aminomethane (Tris), a buffer component, was determined at 1.65 Å resolution. The protein crystallized at 20°C and diffraction data were collected from the crystals to a resolution of 1.65 Å. The crystal belonged to the tetragonal space group P41212, with unit-cell parameters a = b = 61.32, c = 208.57 Å. Analysis of the crystal structure revealed C4—C5—C5A—O4P (77°) and C5—C5A—O4P—P (–143°) torsion angles in the phosphate-group moiety of the cofactor pyridoxal 5′-phosphate (PLP) that are more reasonable than those observed in the previously reported crystal structure (14° and 151°, respectively). Furthermore, the clear electron density showing a Schiff-base linkage between PLP and the bulky artificial ligand Tris indicated exceptional flexibility of the active-site cavity of this enzyme. These findings open up the possibility for further study of the detailed mechanisms of substrate recognition and catalysis by this enzyme.
Determinants of user's intentions to book hotels: a comparison of websites and mobile apps
Authors: Ali Faizan , Ali Laiba , Gao Zhaoyu , Terrah Abraham , Turktarhan Gozde 
Published: November 2022

PurposeThis empirical study uses the stimulus-organism-response (S-O-R) framework to examine the interrelationships amongst hotel websites and app quality, flow, telepresence, user engagement and booking intentions.Design/methodology/approachData from two different datasets, including users of hotel websites (N sample 1 = 257) and hotel mobile apps (N sample 2 = 292), were collected. Partial least squares (PLS-SEM) was used to test the research model.FindingsFindings indicate that the quality of the hotel websites and mobile apps positively influences telepresence, flow and engagement. Telepresence and flow positively affect the users booking intentions for both the samples. However, for hotel website users, engagement has a no-significant effect on booking intentions. Finally, telepresence has a non-significant effect on flow, and flow has a non-significant effect on engagement for both the users of hotel websites and mobile apps.Originality/valueThis study uses two datasets to understand how hotel booking channel (hotel website and mobile app) quality leads to booking intentions by tapping into telepresence, flow and engagement.
Exploring the adoption of technology against delays in construction projects
Authors: Gurgun Asli Pelin , Koc Kerim , Kunkcu Handan 
Published: November 2022

PurposeCompleting construction projects within the planned schedule has widely been considered as one of the major project success factors. This study investigates the use of technologies to address delays in construction projects and aims to address three research questions (1) to identify the adopted technologies and proposed solutions in the literature, (2) to explore the reasons why the delays cannot be prevented despite disruptive technologies and (3) to determine the major strategies to prevent delays in construction projects.Design/methodology/approachIn total, 208 research articles that used innovative technologies, methods, or tools to avoid delays in construction projects were investigated by conducting a comprehensive literature review. An elaborative content analysis was performed to cover the implemented technologies and their transformation, highlighted research fields in relation to selected technologies, focused delay causes and corresponding delay mitigation strategies and emphasized project types with specific delay causes. According to the analysis results, a typological framework with appropriate technological means was proposed.FindingsThe findings revealed that several tools such as planning, imaging, geo-spatial data collection, machine learning and optimization have widely been adopted to address specific delay causes. It was also observed that strategies to address various delay causes throughout the life cycle of construction projects have been overlooked in the literature. The findings of the present research underpin the trends and technological advances to address significant delay causes.Originality/valueDespite the technological advancements in the digitalization era of Industry 4.0, many construction projects still suffer from poor schedule performance. However, the reason of this is questionable and has not been investigated thoroughly.
Academic performance and work placements: does academic performance influence the decision to complete a work placement?
Authors: McCann Michael , Hewitt Michael 
Published: November 2022

PurposeThere is strong evidence that year-long work placements make students more employable and produces better academic performance. Despite this, UK participation rates remain stubbornly low. The authors examine the influence of academic performance on students' willingness and ability to complete work placements.Design/methodology/approachThis study’s novel conceptual framework distinguishes students by their intentions regarding work placements indicated at enrolment as well as whether they completed a work placement. The authors use a sample of 226 business and economics students, employing propensity score weighted multiple regression to analyse the influence of academic performance.FindingsThe results indicate that academic performance has a significant influence on the decision to include a work placement option at enrolment. For those students who do pursue work placements, first-year academic performance had a significantly positive impact on their ability to secure a placement job. Finally, completion of a work placement was beneficial to final year academic performance.Practical implicationsWork placements are beneficial. Since low academic performance deters students from pursuing such opportunities, universities may need to communicate the benefits better to encourage greater interest. Further, universities need to realistically manage the expectations of students with low academic performance who want to do work placements and provide targeted support during the application process. Furthermore, alternatives to work placements should be provided.Originality/valueThis research adds to the literature investigating the influence of academic performance through academic self-concept on students' investment decisions to include a work placement in their degree study and in students' ability to secure a work placement.
Challenges facing procurement and supply professions in Tanzania: higher education students' perceptions
Authors: Changalima Ismail Abdi , Mwaiseje Shadrack Samwel , Mchopa Alban Dismas , Panga Faustine Peter 
Published: November 2022

PurposeDespite the fact that students in higher education contributes significantly to the number of professionals, little has been done to incorporate the students’ perspectives on the status of procurement and supply professions in Tanzania. This study examined the challenges confronting the procurement and supply professions from the perspective of future procurement professionals enrolled in Tanzanian higher education.Design/methodology/approachA cross-sectional design was used to collect data from undergraduate students majoring in procurement and supply chain management in Tanzanian higher education using a structured questionnaire.FindingsThe findings revealed that the challenges affecting the procurement and supply professions were buyer- and supplier-related challenges as perceived by Tanzanian higher education students.Research limitations/implicationsThe study's findings should be generalized with caution because the findings represent the perspectives of Tanzanian procurement and supply undergraduate business students. Furthermore, the included sample of procurement and supply undergraduates cannot represent all students' perceptions; thus, other studies may broaden the sample by including the opinions of other higher education students.Originality/valueThis is the first study that looks at how students in higher education institutions, who are considered future procurement and supply professionals, perceive the challenges of the procurement and supply professions in Tanzania.
Speaking up as an extension of socio-cultural dynamics in hospital settings: a study of staff experiences of speaking up across seven hospitals
Authors: Pavithra Antoinette , Mannion Russell , Sunderland Neroli , Westbrook Johanna 
Published: November 2022

PurposeThe study aimed to understand the significance of how employee personhood and the act of speaking up is shaped by factors such as employees' professional status, length of employment within their hospital sites, age, gender and their ongoing exposure to unprofessional behaviours.Design/methodology/approachResponses to a survey by 4,851 staff across seven sites within a hospital network in Australia were analysed to interrogate whether speaking up by hospital employees is influenced by employees' symbolic capital and situated subjecthood (SS). The authors utilised a Bourdieusian lens to interrogate the relationship between the symbolic capital afforded to employees as a function of their professional, personal and psycho-social resources and their self-reported capacity to speak up.FindingsThe findings indicate that employee speaking up behaviours appear to be influenced profoundly by whether they feel empowered or disempowered by ongoing and pre-existing personal and interpersonal factors such as their functional roles, work-based peer and supervisory support and ongoing exposure to discriminatory behaviours.Originality/valueThe findings from this interdisciplinary study provide empirical insights around why culture change interventions within healthcare organisations may be successful in certain contexts for certain staff groups and fail within others.
The (commercialised) experience of operating: Embodied preferences, ambiguous variations and explaining widespread patient harm
Authors: Ducey Ariel , Donoso Claudia , Ross Sue , Robert Magali 
Published: November 2022

AbstractThis article provides a detailed account of how surgeons perceived and used a device‐procedure that caused widespread patient harm: transvaginal mesh for the treatment of pelvic floor disorders in women. Drawing from interviews with 27 surgeons in Canada, the UK, the United States and France and observations of major international medical conferences in North America and Europe between 2015 and 2018, we describe the commercially driven array of operative variations in the use of transvaginal mesh and show that surgeons’ understanding of their hands‐on, sensory experience with these variations is central to explaining patient harm. Surgeons often developed preferences for how to manage actual and anticipated dangers of transvaginal mesh procedures through embodied operative adjustments, but collectively the meaning of these preferences was fragmented, contested and deferred. We critically reflect on surgeons’ understandings of their operative experience, including the view that such experience is not evidence. The harm in this case poses a challenge to some ways of thinking about uncertainty and errors in medical sociology, and calls for attention to a specific feature of surgical work: the extent and persistence of operative practices that elude classification as right or wrong but are still most certainly better and worse.
Phase II study of durvalumab (MEDI 4736) plus carboplatin and etoposide in elderly patients with extensive stage small cell lung cancer: Study protocol of turtle study (LOGIK 2003)
Authors: Ishii Hidenobu , Azuma Koichi , Shimose Takayuki , Yoshioka Hiroshige , Kurata Takayasu , Shingu Naoki , Okamoto Masaki , Kawashima Yosuke , Okamoto Isamu 
Published: November 2022

AbstractBackgroundRecently, the addition of antiprogrammed cell death‐ligand 1 (PD‐L1) monoclonal antibodies, including durvalumab and atezolizumab to platinum‐based chemotherapy, has demonstrated clinical benefits in patients with untreated advanced small cell lung cancer (SCLC). However, these clinical trials comprised small populations of elderly patients with SCLC. Therefore, the safety of anti‐PD‐L1 immunotherapy plus platinum and etoposide in elderly patients remains unclear.MethodsThis prospective, multicenter, single‐arm study was designed to evaluate the safety and efficacy of durvalumab plus carboplatin and etoposide in untreated elderly patients (aged > 75) with extensive stage (ES) SCLC. A total of 40 patients were recruited. Patients received up to four cycles of durvalumab 1500 mg and carboplatin at a dose equivalent to an area under the curve of 5 on day 1, and etoposide 80 mg/m2 on days 1 to 3 every 3 weeks as induction treatment, followed by durvalumab maintenance treatment every 4 weeks. The primary endpoint was safety as measured by adverse events according to the Common Terminology Criteria for Adverse Events version 5.0, laboratory analyses, vital signs, and physical examination. Key secondary endpoints were objective response rate, median progression‐free survival, 12‐month overall survival rate, and the completion rate for four cycles of induction chemotherapy.DiscussionThe present study was designed to evaluate the safety of durvalumab plus carboplatin and etoposide in elderly patients with ES‐SCLC.
Environmental enrichment alleviates cognitive and psychomotor alterations and increases adult hippocampal neurogenesis in cocaine withdrawn mice
Authors: Mañas‐Padilla M. Carmen , Tezanos Patricia , Cintado Elisa , Vicente Lucía , Sánchez‐Salido Lourdes , Gil‐Rodríguez Sara , Trejo José L. , Santín Luis J. , Castilla‐Ortega Estela 
Published: November 2022

AbstractCocaine is a widely used psychostimulant drug whose repeated exposure induces persistent cognitive/emotional dysregulation, which could be a predictor of relapse in users. However, there is scarce evidence on effective treatments to alleviate these symptoms. Environmental enrichment (EE) has been shown to be associated with improved synaptic function and cellular plasticity changes related to adult hippocampal neurogenesis (AHN), resulting in cognitive enhancement. Therefore, EE could mitigate the negative impact of chronic administration of cocaine in mice and reduce the emotional and cognitive symptoms present during cocaine abstinence. In this study, mice were chronically administered with cocaine for 14 days, and control mice received saline. After the last cocaine or saline dose, mice were submitted to control or EE housing conditions, and they stayed undisturbed for 28 days. Subsequently, mice were evaluated with a battery of behavioural tests for exploratory activity, emotional behaviour, and cognitive performance. EE attenuated hyperlocomotion, induced anxiolytic‐like behaviour and alleviated cognitive impairment in spatial memory in the cocaine‐abstinent mice. The EE protocol notably upregulated AHN in both control and cocaine‐treated mice, though cocaine slightly reduced the number of immature neurons. Altogether, these results demonstrate that EE could enhance hippocampal neuroplasticity ameliorating the behavioural and cognitive consequences of repeated administration of cocaine. Therefore, environmental stimulation may be a useful strategy in the treatment cocaine addiction.
A fear‐based view of wisdom: The role of leader fear of failure and psychological empowerment
Authors: Solansky Stephanie T. , Wang Yuan , Quansah Emmanuel 
Published: November 2022

AbstractLeader wisdom is crucial to effective organizations because it is one of the greatest human capacities. However, understanding what factors impact leader wisdom is still developing. In this paper, we rely on a fear‐based view of wisdom and empirically examine through a quantitative study of 249 leaders if one of the primary regulators of human behavior (fear) is positively related to wisdom. We are specifically focused on the role of fear of failure and wisdom. Additionally, because we recognize that fear has a negative connotation and has a tipping point regarding leader effectiveness, we propose that psychological empowerment serves as a coping mechanism for fear and mediates the relationship between fear of failure and wisdom. Through structural equation modeling (SEM), we demonstrate that fear of failure does positively impact leader wisdom and that this relationship is mediated by psychological empowerment. This study contributes to the literature by advancing the fear‐based view of wisdom which unites two of the most powerful concepts in human history: fear and wisdom. Although wisdom has a resurgence of interest in business literature, fear is not given much attention. By theoretically and empirically linking fear of failure with wisdom, we shed light on how fear is an important motivating mechanism. The study also contributes to practice by suggesting leaders should fear failure and avoid minimizing fears to be wise.
Absence of copy number gain ofEGFR: A possible predictive marker of long‐term response to afatinib
Authors: Nakamura Tomomi , Sato Akemi , Nakashima Chiho , Abe Tomonori , Iwanaga Kentaro , Umeguchi Hitomi , Kawaguchi Atsushi , Sueoka‐Aragane Naoko 
Published: November 2022

AbstractTreatment efficacy of epidermal growth factor receptor tyrosine kinase inhibitor (EGFR‐TKI) is diverse even in non‐small cell lung cancer (NSCLC) patients withEGFRactivating mutations. Extraordinary long‐term responses sustained over 3 years among NSCLC patients treated with afatinib, an EGFR‐TKI, have been reported, but how to predict such long survivors has not been clarified. A multi‐institutional prospective observational study, based on comprehensive genomic examination performed with next‐generation sequencing of circulating tumor DNA (ctDNA), was conducted to identify potential predictive markers of long‐term response to afatinib. Twenty‐nine patients with advanced stage NSCLC andEGFRdriver mutations detected by standard techniques were enrolled in the study. ctDNA from plasma collected before afatinib treatment was analyzed by Guardant360. ctDNA was detected in 25 of the 29 samples. Median progression‐free survival was shorter in patients whose tumors hadEGFRcopy number gain (7.0vs23.0 months,p = 0.022). The impact ofEGFRcopy number on cell proliferation and the antitumor effect of afatinib were evaluated using genome‐editing lung cancer cell lines. HCC827 withEGFRamplification was relatively resistant to afatinib at concentrations below 0.5 nM, but genome‐edited derivatives of HCC827 with decreasedEGFRcopy number demonstrated growth inhibition with 0.1 nM afatinib. The absence ofEGFRcopy number gain detected in ctDNA may be a predictive marker of long‐term response to afatinib. Comprehensive genomic analysis could lead to a more accurate prediction of EGFR‐TKI efficacy.
Topical eye medications causing allergic contact dermatitis
Authors: Ahlström Malin Glindvad , Skov Lone , Heegaard Steffen , Zachariae Claus , Garvey Lene Heise , Johansen Jeanne Duus 
Published: November 2022

AbstractBackgroundAllergic contact dermatitis (ACD) in the eye region caused by topical eye medications is difficult to diagnose and may be overlooked.ObjectiveTo study the characteristics and causative agents in patients with ACD caused by topical eye medications in a Danish tertiary dermatology department.MethodsA retrospective study of 318 patients, patch tested between 2013 and 2021 due to suspected ACD to topical eye medications. All patients were tested with a locally developed eye medication series, some were additionally tested with suspected eye medications. Medical records were studied in patch test positive patients.ResultsContact allergy to a topical eye allergen/medication was found in 12.9% (n = 41) of 318 patients, and culprit allergens were phenylephrine (6.9%), timolol (2.5%) and ketotifen (1.6%). Patch test positive patients were often previously diagnosed with cataract (29.3%) or glaucoma (24.4%), and the majority reported more than one previous reaction. Initial symptoms were oedema (56.0%), erythema (48.8%) and dermatitis (31.7%) in the eye region, and facial dermatitis was also seen.ConclusionsPatients with symptoms from the eye region who have been using topical eye medications should be patch tested with ingredients from commonly used eye medications supplemented by the products tested ‘as is’.
Assessing the interaction between L‐dopa and γ‐transcranial alternating current stimulation effects on primary motor cortex plasticity in Parkinson's disease
Authors: Guerra Andrea , D'Onofrio Valentina , Asci Francesco , Ferreri Florinda , Fabbrini Giovanni , Berardelli Alfredo , Bologna Matteo 
Published: November 2022

AbstractL‐dopa variably influences transcranial magnetic stimulation (TMS) parameters of motor cortex (M1) excitability and plasticity in Parkinson's disease (PD). In patients OFF dopaminergic medication, impaired M1 plasticity and defective GABA‐A‐ergic inhibition can be restored by boosting gamma (γ) oscillations via transcranial alternating current stimulation (tACS) during intermittent theta‐burst stimulation (iTBS). However, it is unknown whether L‐dopa modifies the beneficial effects of iTBS‐γ‐tACS on M1 in PD. In this study, a PD patients group underwent combined iTBS‐γ‐tACS and iTBS‐sham‐tACS, each performed both OFF and ON dopaminergic therapy (four sessions in total). Motor evoked potentials (MEPs) elicited by single TMS pulses and short‐interval intracortical inhibition (SICI) were assessed before and after iTBS‐tACS. We also evaluated possible SICI changes during γ‐tACS delivered alone in OFF and ON conditions. The amplitude of MEP elicited by single TMS pulses and the degree of SICI inhibition significantly increased after iTBS‐γ‐tACS. The amount of change produced by iTBS‐γ‐tACS was similar in patients OFF and ON therapy. Finally, γ‐tACS (delivered alone) modulated SICI during stimulation and this effect did not depend on the dopaminergic condition of patients. In conclusion, boosting cortical γ oscillatory activity via tACS during iTBS improved M1 plasticity and enhanced GABA‐A‐ergic transmission in PD patients to the same extent regardless of dopaminergic state. These results suggest a lack of interaction between L‐dopa and γ‐tACS effects at the M1 level. The possible neural substrate underlying iTBS‐γ tACS effects, that is, γ‐resonant GABA‐A‐ergic interneurons activity, may explain our findings.