bluuebunny/crossref_metadata_2025_split huggingface dataset:
On usage of artificial intelligence for predicting mortality during and post-pregnancy: a systematic review of literature
Authors: Rocha Elisson da Silva , Melo Flávio Leandro de Morais , de Mello Maria Eduarda Ferro , Figueirôa Barbara , Sampaio Vanderson , Endo Patricia Takako 
Published: September 2022

Abstract
Background: Care during pregnancy, childbirth and puerperium are fundamental to avoid pathologies for the mother and her baby. However, health issues can occur during this period, causing misfortunes, such as the death of the fetus or neonate. Predictive models of fetal and infant deaths are important technological tools that can help to reduce mortality indexes. The main goal of this work is to present a systematic review of literature focused on computational models to predict mortality, covering stillbirth, perinatal, neonatal, and infant deaths, highlighting their methodology and the description of the proposed computational models.
Methods: We conducted a systematic review of literature, limiting the search to the last 10 years of publications considering the five main scientific databases as source.
Results: From 671 works, 18 of them were selected as primary studies for further analysis. We found that most of works are focused on prediction of neonatal deaths, using machine learning models (more specifically Random Forest). The top five most common features used to train models are birth weight, gestational age, sex of the child, Apgar score and mother's age. Having predictive models for preventing mortality during and post-pregnancy not only improve the mother's quality of life, as well as it can be a powerful and low-cost tool to decrease mortality ratios.
Conclusion: Based on the results of this SRL, we can state that scientific efforts have been done in this area, but there are many open research opportunities to be developed by the community.
5-azacytidine inhibits the tumorigenesis of esophageal cancer cells through the suppression of NOTCH1 signaling
Authors: Xu Tong , Hu Li-na , Yu Minghua 
Published: September 2022

Abstract
Background: Esophageal squamous carcinoma (ESCC) and esophageal adenocarcinoma (EAC) are different pathological types of esophageal cancer (EC) with low patient survival. The methyltransferase inhibitor 5-azacytidine (5-azaC) has been approved to treat hematological malignancies and malignant solid tumors for years. NOTCH1 pathway plays an important role in both hematological and esophageal cancer and previous studies demonstrated a NOTCH1/IL-7/IL-7R signal in other cancers.
Methods: TE-1 and OE33 cells were employed to represent ESCC and EAC respectively. The effects of 5-azaC on cells were evaluated by CCK8, wound healing, Transwell assay, and flow cytometry. Pyrosequencing was performed to detect changes of 18 CpG units in cells after being treated with 5-azaC. Western blot and Quantitative Real-time PCR were conducted respectively to test expressions of NOTCH1/IL-7/IL-7R signal for exploring the mechanisms. siRNA transfections were performed to inhibit IL-7R.
Results: 5-azaC showed anticancer effects and NOTCH1 signaling was also downregulated in both cell lines. Although there were abundant CpG islands in NOTCH1, no change was observed in its methylation level. Moreover, the combination of 5-azaC with NOTCH1 signaling inhibitor DAPT had a synergistic inhibiting effect in EAC but ESCC cells. We proved the existence of the NOTCH1/IL-7/IL-7R signal in the ESCC cell line. Additionally, the activation or inactivation of the IL-7/IL-7R pathway could mitigate or potentiate the potency of 5-azaC on ESCC cells as well.
Conclusions: Our findings showed a possibility of treating esophageal cancer with 5-azaC combining inhibitors of NOTCH1/IL-7/IL-7R signal, hoping to provide novel therapeutic strategies for EC.
Orthorexia Nervosa Tendencies of Liver Transplant Patients Receiving Immunosuppressant Treatment: A Cross-Sectional Study
Authors: Kartal Mert , Kapikiran Gurkan , Karakas Nese 
Published: September 2022

Abstract
One of the most important factors after organ transplantation is immunotherapy, which is effective in reducing immune response to prevent post-transplant graft rejection, while another factor is a balanced nutrition. With these considerations in mind, this study aimed to determine the orthorexia nervosa tendencies of liver transplant patients receiving immunosuppressant treatment. This descriptive and cross-sectional study was carried out with patients who underwent liver transplantation at the liver transplantation institute of a university hospital in eastern Turkey. A sociodemographic information form and ORTO-R were used to collect data. The data were collected from patients who attended their follow-ups between May and August 2022. The mean age of the 176 patients who were receiving immunosuppressive treatment was 58.55±7.56, while 86.4% had not received any education on nutrition. The mean ORTO-R score of the patients was 17.09±2.70, which indicated moderate orthorexia. In our study, it was concluded that the majority of the patients who were using immunosuppressive medication had not received any education on nutrition, and they were moderately orthorexic. It should be kept in mind that this obsessive form of eating will affect balanced nutrition among patients who have gone through a major transplant process. To make the drug-diet interaction in patients using immunosuppressive drugs after organ transplantation beneficial, ensure that they have a sufficient and balanced diet, and make this diet sustainable, it is important to monitor these patients. Additionally, it is thought that evaluating the pre-transplant orthorexia nervosa tendencies of advanced-stage liver transplant patients will increase post-transplant success.
Level of evidence: Level V. Cross-sectional survey-based study
Urine cell-free DNA multi-omics to detect MRD and predict survival in bladder cancer patients
Authors: Chaudhuri Aadel , Chauhan Pradeep , Shiang Alexander , Alahi Irfan , Sundby R , Feng Wenjia , Gungoren Bilge , Nawaf Cayce , Babbra Ramandeep , Harris Peter , Qaium Faridi , Hatscher Casey , Antiporda Anna , Brunt Lindsey , Mayer Lindsey , Shern Jack , Baumann Brian , Kim Eric , Reimers Melissa , Smith Zachary 
Published: September 2022

Abstract
Circulating tumor DNA sensitivity remains subpar for minimal residual disease (MRD) detection in bladder cancer patients. To remedy this, we focused on the biofluid most proximal to the disease, urine, and analyzed urine tumor DNA (utDNA) in 74 localized bladder cancer patients. We integrated ultra-low-pass whole genome sequencing (ULP-WGS) with urine Cancer Personalized Profiling by deep Sequencing (uCAPP-Seq) to achieve sensitive MRD detection and predict overall survival. Variant allele frequency, inferred tumor mutational burden, and tumor fraction levels in urine cfDNA significantly predicted pathologic complete response status. A random forest model integrating these factors with leave-one-out cross-validation was 87% sensitive for predicting MRD. Both progression-free survival (HR=3.00, p=0.01) and overall survival (HR=4.81, p=0.009) were dramatically worse by Kaplan-Meier analysis for patients predicted by the model to have MRD, which was corroborated by Cox regression analysis. Additional survival analyses performed on muscle-invasive, neoadjuvant chemotherapy, and held-out validation subgroups corroborated these findings. In summary, we profiled urine samples from 74 patients with localized bladder cancer and used urine cell-free DNA multi-omics to sensitively detect MRD and accurately predict survival.
Depression symptoms among New Zealand’s Asian community in the first 10 months of the COVID-19 pandemic
Authors: Siegert Richard J. , Ran Guanyu Jason , Zhu Andrew , French Nigel , Johnston David , Liu Liangni Sally 
Published: September 2022

Abstract
Background
The COVID-19 pandemic has elevated levels of distress and resulted in anti-Asian discrimination in many countries. We aimed to determine the 10-month prevalence of depression symptoms in Asian adults in New Zealand during the pandemic and to see if this was related to experience of racism.
Methods
Online survey of 1,101 Asians with a 36.5% response rate. A stratified sample of 402 respondents completed the brief Centre for Epidemiological Studies-Depression (CES-D) scale. Analyses included: descriptive statistics, depression scores by age/gender, factor analysis of the 10 item CES-D and partial correlation network analysis of CES-D items together with questions about experience of racism.
Results
Half the sample reported clinically significant symptoms of depression over the past 10 months. Depression was higher among younger participants but there was no gender difference. Internal consistency was high (α = 0.85) for the CES-D which revealed a clear two factor structure. Network analysis suggested that sleeping problems might be the bridge between experiences of racism and depression. However, this study was cross-sectional which prevents any definitive inferences about causality.
Conclusions
The prevalence of low mood was high with half the sample scoring above the cut-off point for clinically significant levels of depressive symptoms. Depression was higher in younger people and had a modest positive correlation with personal experience of racism - possibly due to its effect on sleep. Longitudinal studies that examine a broader range of dimensions of distress are needed.
Spectrum and clinical features of gene mutations in Chinese pediatric acute lymphoblastic leukemia
Authors: Shen Diying , Liu Lixia , Xu Xiaojun , Song Hua , Zhang Jingying , Xu Weiqun , Zhao Fenying , Liang Juan , Liao Chan , Wang Yan , Xia Tian , Wang Chengcheng , Lou Feng , Cao Shanbo , Qin Jiayue , Tang Yongmin 
Published: September 2022

Abstract
Purpose
The 5-year survival rate of children with acute lymphoblastic leukemia (ALL) is 85–90%, with a 10–15% rate of treatment failure. Next-generation sequencing (NGS) identified recurrent mutated genes in ALL that might alter the diagnosis, classification, prognostic stratification, treatment, and response to ALL. Few studies on gene mutations in Chinese pediatric ALL have been identified. Thus, an in-depth understanding of the biological characteristics of these patients is essential.. The present study aimed to characterize the spectrum and clinical features of recurrent driver gene mutations in a single-center cohort of Chinese pediatric ALL.
Methods
We enrolled 219 patients with pediatric ALL in our single center. Targeted sequencing based on NGS was used to detect gene mutations in patients. The correlation was analyzed between gene mutation and clinical features, including patient characteristics, cytogenetics, genetic subtypes, risk stratification and treatment outcomes using χ2-square test or Fisher’s exact test for categorical variables.
Results
A total of 381 gene mutations were identified in 66 different genes in 152/219 patients. PIK3R1 mutation was more common in infants (p = 0.021). KRAS and FLT3 mutations were both more enriched in patients with hyperdiploidy (P < 0.0001, P = 0.0003, respectively). NRAS, PTPN11, FLT3, and KMT2D mutations were more common in patients who did not carry the fusion genes (all p < 0.05). PTEN mutation was significantly associated with high-risk ALL patients (P = 0.011), while NOTCH1 mutation was common in middle-risk ALL patients (P = 0.039). Patients with ETV6 or PHF6 mutations were less sensitive to steroid treatment (P = 0.033, P = 0.048, respectively).
Conclusion
This study depicted the specific genomic landscape of Chinese pediatric ALL and revealed the relevance between mutational spectrum and clinical features of Chinese pediatric ALL, which highlights the need for molecular classification, risk stratification, and prognosis evaluation.
Identification of the targeted mRNA associated with the miRNA microarray of chemo-sensitive choriocarcinoma using bioinformatics analysis
Authors: Zhang Taohong , Yang Lei , Xu Huiqiu , Hao Jiatao , Yu Hui , An Ruifang , Xue Yan 
Published: September 2022

Abstract
Background
MicroRNAs (miRNAs) play an important and necessary role in the occurrence and development of various tumours. The aim of our study was to identify miRNAs as new biomarker of choriocarcinoma (CC) chemosensitivity.
Methods
We performed GeneChip hybridization of samples of trophoblastic villi and chemosensitive CC tissues, and differentially expressed miRNAs between the groups were obtained. The possible target genes of the most highly 3 overexpressed and underexpressed miRNAs were investigated through bioinformatics analysis. Then, the enriched pathways and biological functions of the hub genes associated with miRNAs were analysed, and networks were constructed by Cytoscape.
Results
A total of 148 differentially expressed miRNAs were identified from the GeneChip tissue hybridization analysis. The top 3 overexpressed and underexpressed miRNAs were miR‑100-5p, miR-432-5p and miR-144-3p, and miR-512-5p, 515‑5p and 519c‑3p, respectively. In the protein − protein interaction (PPI) networks, the top 18 genes with the highest degree of connectivity with overexpressed and underexpressed miRNAs were selected as hub genes. PTEN was identified as the key hub gene in the miRNAhub gene networks and to be potentially modulated by miR144-3p and miR-519c3p.
Conclusion
According to the results of our bioinformatics analysis, two miRNAs, miR144-3p and miR-519c-3p, may be potential biomarkers of and PTEN may play a crucial role in CC chemosensitivity.
N-acetylaspartate-Like Peak on MR Spectroscopy – A Useful Clue For Brain Metastases that Originate from Mucin- Expressing Primary?
Authors: Ostojic Jelena , Kozic Dusko , Grujicic Danica , Brkic Biljana Georgievski , Dragicevic Dusan , Boban Jasmina 
Published: September 2022

Abstract
Differential diagnosis between high-grade glioma and solitary cerebral metastasis remains to be diagnositc challenge using conventional imaging techniques. Magnetic resonance spectroscopy (MRS) is an advanced clinical magnetic resonance imaging technique that might help in resolivng this dillemma. Here we present a pictorial case series of 5 patients with observed peak close to the resonance of N-acetyl aspartate in the secondary tumors of various origin, potentially associted with the expression of mucin. Two patients had melanoma metastasis, one non-small-cell lung cancer metastasis, one metastasis from oropharyngeal cancer and one from the breast cancer. In all patients contrast-enhanced magnetic resonance imaging of the brain showed solitary infiltrative lesion. MRS was performed in all lesions, and showed dominant peak close to 2.0ppm, and was reported as “N-acetyl-aspartate-like”. Based on the existing knowledge, this peak probably represents N-acetyl glycoproteins, as a reflection of mucin expression on the cancer cells. Thus, this dominant peak might be a very useful clue for attributing the single ring-enhancing lesions to the secondary dissemination of a mucin-expressing producing primary cancers, including melanoma, breast cancer, non-small cell lung cancers and oropharyngeal carcinoma, rather than to a high-grade glioma.
Three-dimensional finite element model analysis of biomechanical differences between classic suture bridge technique and modified "fishing net" suture bridge technique for rotator cuff
Authors: Wang Chen , Gao Ao-Fei , Pan Hai-Le 
Published: September 2022

Abstract
Background
The classic suture bridge technique is one of the most commonly used techniques for the treatment of rotator cuff tears, but there are still some defects when repairing larger rotator cuff tear
Methods
The CT and MRI scan data of the shoulder joint of a healthy adult were imported into Mimics 21.0, 3-matic 11.0, Geomagic studio 2012 and UG NX 9.0 to establish the models of the two surgical methods in turn, and then imported into Hypermesh 2019 to complete the pretreatment operations such as material endowment, meshing, applied load and boundary conditions. Finally, Abaqus 6.14 software was imported to compare the characteristics of stress distribution, peak stress, and mean stress of the two surgical methods at 5 °, 10 °, 15 °, 20 °, 25 °, and 30 ° of simulated shoulder abduction.
Results
At shoulder abduction of 5 °, 10 °, 15 °, 20 °, 25 °, and 30 °, the stress in both surgical models was mainly concentrated around the tendon passed by the suture, but the peak stress in the supraspinatus muscle of the modified "fishing net" suture bridge technique was less than that of the classical suture bridge. And when shoulder abduction was 20 °, 25 °, and 30 °, the mean stress of the modified "fishnet" suture bridge technique was significantly smaller than that of the classical suture bridge technique, and the difference was statistically significant (p < 0.05). At 5 °, 10 °, and 15 ° of abduction, the difference was not statistically significant (p > 0.05), but the values remained lower than in the classic suture-bridge technique.
Conclusion
The special suture distribution in the modified "fishing net" suture bridge technique can provide better anatomical coverage without increasing the number of anchors, effectively avoid the generation of deformity, and has a more uniform stress distribution inside the tendon, which is biomechanically superior to the classical suture bridge technique and can be used as a technical means in the repair of large rotator cuff tears.
TNF-α contributes to sarcopenia through caspase-8/caspase-3/GSDME-mediated pyroptosis
Authors: Wu Jingying , Lin Siming , Chen Weixiao , Lian Guili , Wu Weibin , Chen Ai , HAJARY SAGOR MOHAMMAD ISMAIL , Luo Li , Wang Huajun , Xie Liangdi 
Published: September 2022

Abstract
Sarcopenia has become a leading cause of disability and mortality in the elderly. Decreased size and loss of the number of skeletal muscle cells are the histological manifestations of sarcopenia. Increasing evidence shows that programmed cell death (PCD) contributes to the loss of skeletal muscle fibers. TNF-α is also validated to play a prominent role in sarcopenia through its complex signaling pathways including cell death signaling. However, it is unclear whether TNF-α contributes to sarcopenia by mediating pyroptosis which is one type of PCD. Here, we first established naturally aged mice with sarcopenia model and confirmed an inflammatory state represented by TNF-α in aged mice. Evidence of GSDME-mediated pyroptosis and activation of apoptotic caspase-8/-3 were further found in skeletal muscle cells. We demonstrated that TNF-α triggered GSDME-mediated pyroptosis in myotubes through activating caspase-8 and caspase-3 by using caspase-8 and caspase-3 inhibitors. Comparing the activation of caspase-8 and GSDME expression between TNF Complex IIa and TNF Complex IIb, TNF-α was more inclined to assemble TNF Complex IIb in activating caspase-8 and triggering pyroptosis. Moreover, pyroptotic myotubes were validated to result in decreased expression of MHC1 and finally loss of myotubes by knockdown of GSDME. Our work unravels a novel mechanism that TNF-ɑ/caspase-8/caspase-3/GSDME signaling-mediated pyroptosis plays a pathogenic role in the development of sarcopenia. caspase-3/GSDME signaling-mediated pyroptosis can be a promising therapeutic target for sarcopenia.
“Ariadne’s thread”. Psycho-educational empowerment intervention for patients with metastatic breast cancer. A Case Study
Authors: Alfieri Sara , Murru Luciana , Bosisio Marco , Gangeri Laura , Monfredini Michela , Capri Giuseppe , Lozza Laura , Borreani Claudia 
Published: September 2022

Abstract
Background.
“Ariadne’s thread” is a psycho-educational intervention designed by the Clinical Psychology Unit of an Italian Comprehensive Cancer Center and aimed at promoting empowerment in patients with metastatic breast cancer. It consists of 8 online meetings led by a psycho-oncologist where informational parts discussed by all the doctors that care these patients alternate with moments of stress management.
Aim.
This case study aims to investigate: 1) the feasibility of the "Ariadne's thread" intervention; 2) the satisfaction and perception of efficacy of the pilot intervention by the participants.
Method.
We used a mixed method in which 1) it was detected: the number of acceptance to the intervention both of patients and professionals, the number of help requests by patients and the number of requests of date changing by the professionals; 2) semi-structured interviews to the professionals who participated at the intervention were conducted; 3) 2 focus groups with the patients were conducted and a questionnaire was submitted to each of them.
Results.
The intervention is sustainable for the organisation, professionals and patients point of view. In particular, the patients declare the perception of efficacy in many aspects: improvement of the relation with the doctors, acceptance of their illness, learning of a relaxing technique, possibility to look at the world with trust and hope, etc. The questionnaires show an improvement of empowerment and the satisfaction with the intervention.
Conclusion.
Even though other research are needed, the results are promising.
Diagnostic value of CMA in different phenotypes of fetal congenital heart defects
Authors: Zhang Simin , Pei Yan , Wang Jingjing , Han Jijing , Xiong Xiaowei , Yan Yani , zhang Juan , Liu Yan , Su Fangfei , Xu Jinyu , Wu Qingqing 
Published: September 2022

Abstract
Objective
To evaluate the detection rate of fetal chromosomal abnormalities in congenital heart defects (CHD), further dig the potential diagnostic value of Chromosomal Microarray Analysis (CMA) technology for different phenotypes, and explore the possible genetic pathogenic factors of CHD.
Methods
We analyzed the CMA of 427 cases of CHD fetuses, and divided CHD into different groups according to two dimensions. According to whether they were combined with ECA, they were divided into isolated CHD and non-isolated CHD; According to the cardiac phenotype, they were divided into ten groups. The correlation between numerical chromosomal abnormalities (NCA), and copy number variations (CNVs, except likely benign and benign CNVs) with CHD was analyzed by Mantel test.
Results
In general, CHD with ECAs were more likely to have a genetic abnormality than those without ECAs (39.3% vs. 14.8%, P<0.05). The genetic abnormality rate of 427 fetuses was 21.8% (93/427), the detection rate of NCA was 12.9% (55/427), the most relevant were skeletal, Craniofacial, VSD, AVSD (P<0.05); and the detection rate of pCNVs was 8.9% (38/427), the most relevant were IAA, A, IAA, B, RAA, TAPVC, CoA, TOF and thymic abnormality. 22q11.2DS and had the highest detection, the detection rate of 22q11.2DS in the subgroups were IAA, B, RAA, PS, CoA, TOF and thymic abnormality. Overall analysis of cases, the CNV deletion fragment larger than 1 Mb may be pathogenic. In CNV, deletion was more likely to be pathogenic than duplication.
In addition, we detected 12 CNV syndromes; among of them, 9 syndromes that may be related to CHD. gene LIMK1 and MYH11 have been identified as part of a common pathway between cardiovascular and neurological development; candidate genes are considered to be related to heart and/or involved in embryonic development, FLI1, NIPBL, DLL1, PTPN11, TBX5.
Conclusion
Increased risk of genetic abnormalities in non-isolated CHD; CHD phenotype most related to NCA, pCNV and 22q11.2DS was found; and 12 CNV syndromes and 7 meaningful candidate genes were detected.