bluuebunny/crossref_metadata_2025_split huggingface dataset:
Galectin-3 binding protein stimulated IL-6 expression is impeded by antibody intervention in SARS-CoV-2 susceptible cell lines
Authors: Mendes-Frias Ana , Gallo Valentina , Iacobelli Valentina , Gentile Roberta , Antonini Giovanni , Silvestre Ricardo , Iacobelli Stefano 
Published: April 2022

Abstract
COVID-19 is the global pandemic that affected our population in the past two years. Considerable research has been done to better understand the pathophysiology of this disease and to identify new therapeutic targets, especially for severe cases. Galectin-3 (Gal-3) is a receptor present at the surface of different cell types, namely epithelial and inflammatory cells, which has been described as a severity marker in COVID-19. The activation of Gal-3 through its binding protein (Gal-3BP) is directly linked to the production of pro-inflammatory cytokines that contribute for the cytokine storm (CS) observed in severe COVID-19 patients. Here, we show that D2, a recombinant fragment of the lectin-binding region of Gal-3BP was able to stimulate the expression of IL-6 in colon and lung epithelial cell lines in β-galactoside dependent manner. We further show that D2-induced IL-6 augmentation was reduced by the anti-Gal-3BP monoclonal antibody 1959. Our data confirm and extend prior findings of Gal-3BP mediated IL-6 induction, enlightening the potential of its antibody-mediated s blockage for the prevention and treatment of CS and severe disease in COVID-19 patients.
Association of prenatal depression and plasma prolactin concentrations with neonatal birthweight
Authors: Wang Shan , Jia Xiaowen , Zhang Dan , Chen Shengquan , Xu Meina , Jin Xi , Zhu Zhongliang , Li Hui 
Published: April 2022

Abstract
Background: Accumulating evidence suggests that prenatal depression is associated with altered birthweight in the offspring. The hormone prolactin (PRL) plays an important role in the etiology of mood disorders in women, especially for depression. We tested the hypotheses that maternal prenatal PRL levels can significantly influence neonatal birthweight and adequate PRL levels can alleviate prenatal depression-induced normal neonatal birthweight loss risk. Methods: The study population consisted of 392 mother-infant dyads. Symptoms of maternal prenatal depression were evaluated through the Edinburgh Postnatal Depression Scale and concentrations of plasma PRL and placental growth hormone (PGH) were measured before the delivery and umbilical cord plasma levels of insulin-like growth factor -1(IGF1) were measured at birth. The associations among maternal depressed symptoms, concentrations of related hormones and infant's birthweight were examined through linear regressions models. Results: Infants of low maternal PRL had a 0.169 unit (95%CI, -0.488,- 0.130) decrease in the birthweight Z-score compared to infants whose mothers had adequate PRL. Infants of depressed mothers with low PRL had a 0.253 unit(95%CI, -0.853,-0.369) decrease in the birthweight Z score compared to infants whose mothers had adequate PRL and normal emotion. Among infants of depressed mothers, we demonstrated that neonatal birthweight was reduced by a 0.285 unit if the mothers had low PRL concentrations compared to adequate PRL concentrations. As compared with low maternal PRL concentrations, high PRL concentrations were associated with an increase in the concentrations of IGF1 in offspring, but no association with PGH.Conclusion: We found that prenatal depression and low maternal PRL levels are at increased risk of normal infant’ s birthweight loss as measured by weight Z-score and sufficient maternal PRL levels would mitigate the adverse impact of prenatal depression on birthweight. We also found that prenatal depression and low PRL concentrations were associated with reduced fetal IGF1.
Organic Residues in transport vessels from the Red Sea Coast of Eritrea: A Further Element to Understand Past Commercial Relations
Authors: Zerai Abraham , Guerrini Camilla , Łucejko Jeannette J. , Ribechini Erika , Piccirillo Anna , Massa Serena , Mandelli Chiara , Medin Tsegai , Re Alessandro , Giudice Alessandro Lo , Davit Patrizia , Gulmini Monica 
Published: April 2022

Abstract
The archaeological site of Adulis lays on the Red Sea Coast of Eritrea and during Late antiquity played a significant role in interregional commerce among the Mediterranean, the Red Sea and the Indian Ocean coasts. Contacts with the Eastern Mediterranean, Arabian Peninsula and the Sasanian world have been attested from different classes of pottery that were brought to light from on-going excavations at the site. Transport vessels have attracted particular attention as they testify the extent of trades and exchange networks. Transport vessels were coated by organic materials to seal porosity and make them suitable to transport different liquids and/or food. The characterisation of coating materials helped shedding light on their function, and also support the attribution to different classes of transport vessels found in the Indian Ocean and Red Sea worlds. Here, the characterisation of the organic lining detected on a set of samples identified as Late Roman Amphora 1 is discussed. Results from the chemical analyses performed by FT-IR and GC-MS revealed that bitumen was used for lining the jars, thus leading to set the classification of the amphorae within the wide class of the so- called Torpedo jars. By overcoming the question of typological complexity posed from macroscopic examination of the sherds, the chemical investigation contributed here crucial information for the interpretation of past trading in the Indian Ocean. The research gave clues to broaden the distribution of the Torpedo jars to Adulis, giving an unexpected insight into the trading routes of the past.
Comparison of the prevalence and severity of nausea and vomiting in the first trimester between singleton pregnancies conceived from stimulated in vitro fertilization and frozen embryo transfer cycles
Authors: Wong Evelyn , Ko Jennifer KY , Li Raymond HW , Ng Ernest HY 
Published: April 2022

Abstract
Objective The objective of this prospective study is to compare the prevalence and severity of nausea and vomiting in the first trimester between singleton pregnancies conceived from stimulated in vitro fertilization (IVF) and frozen embryo transfer cycles (FET). MethodsAll women were recruited at 6 weeks gestation and filled in the modified Pregnancy-Unique Quantification of Emesis and Nausea (PUQE) to document whether they had any experience of nausea and vomiting weekly till 12 weeks gestation. The primary outcome was the prevalence of nausea and vomiting and the secondary outcomes included severity of nausea and vomiting and pregnancy outcomes. ResultsA total of 360 pregnant women were recruited and 171 were in the stimulated IVF group and 189 in the FET group. The overall return rate was 82.2% (81.8% in the stimulated IVF group and 82.5% in the FET group). Nausea and vomiting were worse in the FET group compared with the IVF group. There were significantly more women who felt nauseated or sick in the FET group (p value=0.032 for week 11 and p value=0.046 for week 12); significantly more women with a longer duration of nausea in the FET group (p value=0.044 for week 7 and p value=0.030 for week 8); significantly more women with more vomiting in a day in the FET group (p value=0.042) and significantly more women with retching or dry heaves in the FET group (p value=0.030 for week 8 and p value=0.028 for week 11). Conclusion Nausea and vomiting were significantly more prevalent and severe in the FET group when compared with the stimulated IVF group.
A SPAST gene-related hereditary spastic paraplegia type 4 family: Identification by a novel mutation
Authors: Zhang Liping , wang Yufen , He Xiaofeng 
Published: April 2022

Abstract
Objective: The aim of this study was to confirm the disease causing of a family with hereditary spastic paraplegia. Methods: Detailed clinical characterization analysis was undertake in the HSP family, taking peripheral blood sample from the 9 subjects for the extraction of genomic DNA and the proband underwent next generation sequencing. Potentially causative variants were identified by Sanger sequencing and multiplex ligation-dependent probe amplification. Results: In this study, we obtained clinical and genetic findings in the family with HSP. There were four generations including 47 members, and ten affected members displayed pure HSP, they showed clinical phenotyping that varied in their severity. Gene sequencing suggested that the proband has carried a novel heterozygous variant c.1057_1058insCC in the SPAST gene, and the same mutation was identified in other affected family members.Conclusions: Here, we showed a family with pure autosomal dominant HSP type 4(SPG4). The novel mutation c.1057_1058insCC of SPAST is the causative variant in this HSP-SPG4 family. And the persent study expands the mutation spectrum of SPAST and provides an opportunity to study the genotype-phenotype correlation in SPG4.
Stochastic bifurcations of nonlinear vibroimpact system with time delay and fractional derivative excited by Gaussian white noise
Authors: Wu Hao , Wang Qiubao , Zhang Congqing , Han Zikun , Tian Ruilan 
Published: April 2022

Abstract
This paper investigates the stochastic dynamics in the nonlinear vibroimpact system with time delay and fractional derivative under Gaussian white noise excitation. Firstly, by dealing with the fractional derivative and using the non-smooth transformation method, the original system is transformed into an equivalent stochastic delay vibroimpact system without fractional derivative. Then, the time delay effect is equivalent to Markov jump system, aiming to eliminate the term of time delay in the equivalent stochastic system, which is one of the highlights in this paper. Another highlight is the modified primary frequency in the optimized system is slightly improved than the previous. At last, by using the stochastic averaging method, the stationary density function of the stochastic Ito ˆ equation is obtained. The agreements between the original system and the optimized system without fractional derivative and the term of time delay validate the effectiveness of the proposed method. We also explore the stochastic P-bifurcation induced by the power spectral density of two uncorrelated noises, time delay, fractional order, and restitution coefficient of the system.
A New Framework for Modelling Seed Germination and Seedling Tillering of Winter Wheat in the Field
Authors: Chen Jinping , Whalley Peter A. , Li Zhongyang , Gao Yang , Zhang Xiaoxian , Hawkesford Malcolm J. , Whalley W. Richard 
Published: April 2022

Abstract
Aims
Seed germination is regulated by multiple environmental cues in the field and understanding their relationship is critical to planning seed drilling and subsequent seedling management but challenging due to the trade-off impact of some environmental stimuli on metabolic reactions involved in the germination.
Methods
We develop a new framework by introducing a physiological dimension over which the metabolic reactions associated with seed germination can be modelled as a moving dynamic event. The influence of environmental cues and the heterogeneity of seeds is described by an average germination rate and a dispersion coefficient. Winter wheat seeds were drilled at different dates in field plots under different soil water contents, and the time course of germination in all treatments and the subsequent tillering development are used to validate the model.
Results
The model accurately reproduces the time course of germination in all treatments. We find that the average germination rate increases asymptotically rather than linearly as the temperature increases within the base-suboptimal temperature range, and that there is an optimal soil water content at which the germination rate peaks due to the trade-off impact of soil water on seed imbibition and soil anaerobicity. The average germination rate and the dispersion coefficient are closely related.
Conclusions
Using soil water content instead of matric potential in our model can naturally describe the simultaneous impact of soil water on soil anaerobicity and seed imbibition, and the physiological dimension can be extended to combine seed germination and seedling tillering development as a continued physiological process.
Fungal Community Succession on Decomposing Leaf Litter Across Five Phylogenetically Related Tree Species in a Subtropical Forest
Authors: Tennakoon Danushka S. , Kuo Chang-Hsin , Purahong Witoon , Gentekaki Eleni , Pumas Chayakorn , Promputtha Itthayakorn , Hyde Kevin D. 
Published: April 2022

Abstract
Fungi are an essential component of the ecosystem. They play an integral role in the decomposition of leaf litter and return nutrients to the ecosystem through nutrient cycling. They are considered as the “key players” in leaf litter decomposition, because of their ability to produce a wide range of extracellular enzymes. Time-related changes of fungal communities during leaf litter decomposition have been relatively well-investigated. However, it has not been established how the tree species, tree phylogeny, and leaf litter chemistry influence fungal communities during decomposition. Using direct observations and a culturing approach, this study compiles fungi found in freshly collected leaf litter from five phylogenetically related, native tree species in Taiwan: Celtis formosana (CF), Ficus ampelas (FA), Ficus septica (FS), Macaranga tanarius (MT), and Morus australis (MA). We investigated (i) the effects of tree species (including tree phylogeny) and leaf litter chemistry on fungal community succession, and (ii) specific patterns of fungal succession (including diversity and taxonomic community assembly) on decomposing leaf litter across the selected tree species. We hypothesized that host species and leaf litter chemistry significantly affect fungal community succession. A total of 1325 leaves (CF: 275, FA: 275, FS: 275, MT: 275 and MA: 225) were collected and 236 fungal taxa were recorded (CF: 48, FA: 46, FS: 64, MT: 42 and MA: 36). Tree species relationships had variable associations on the fungal communities, as even closely related tree species had strongly differing communities during decomposition. A high number of species were unique to a single tree species and may indicate ‘host-specificity’ to a particular leaf litter. The overlap of microfungal species in pair wise comparisons of tree species was low (7–16%), and only 1–2% of microfungal species were observed in leaves of all tree species. The percentage of occurrences of fungal communities using Hierarchical Cluster Analyses (HCA) showed that there were at least four succession stages in each tree species during decomposition. Fungal diversity increased at the beginning of each tree species leaf decay, reached peaks, and declined at the final stages. Overall, our findings demonstrate that tree species and leaf litter chemistry are important variables in determining fungal diversity and community composition in leaf litter. This study also provides a host-fungus database for future studies on these hosts and increases the knowledge of fungal diversity in leaf litter. New fungal discoveries from this study (two new families, two new genera, 40 new species and 56 new host records) were described in our previous publications and are used for comparison here.
Birth outcomes of full-term pregnant women with false labor: A retrospective audit
Authors: Madumise Mikie , Yazbek Mariatha , Filmalter Celia , Botha Tanita 
Published: April 2022

Abstract
Background: The diagnosis of false labor is common. Sending a woman home in early or false labor may result in adverse maternal and neonatal birth outcomes. The study evaluated the birth outcomes of full-term pregnant women following diagnosis of false labor.Methods: A quantitative, retrospective, and descriptive design was conducted in a midwife obstetric unit of a district hospital in South Africa. A hospital customized piloted audit tool was purposively completed from the register in the labor unit of the first 100 records of pregnant women who consulted and were discharged home after false labor from January to May 2019. Descriptive statistics were used to analyze the completed audit tools using frequency tables and charts.Results: None of the women discharged home after being diagnosed with false labor gave birth at home or during transit to the labor unit. Most pregnant women (79%) returned to the hospital between 1 day and 4 weeks for possible birth. Most women (81%) gave birth vaginally. None of the women or babies died, had an obstructed labor or a retained placenta. Only four babies were admitted in the Neonatal Intensive Care Unit. Conclusion: Discharging full-term pregnant women following diagnosis of false labor did not lead to poor birth outcomes. To protect normal birth, all women should be given adequate health education regarding active labor and correct admission protocols should be followed.
Entrance and Origin of the Extracranial Vertebral Artery: CTA Findings
Authors: Yi Xueting , Xie Ping , Zhang Lianwei , Lu Fengxia , Chen Hao , Liu Kefu 
Published: April 2022

Abstract
Objective
To investigated morphological variability of VA origin and its entrance level into cervical transverse foramina by CTA.
Methods
To retrospectively investigated computed tomography angiograms (CTA) of 223 subjects (446 VA courses). Postprocessing of source images was performed by using a multi-planar reformation (MPR), maximum intensity projection (MIP), multi-planar reconstruction (MPR) and volume rendering (VR) algorithms. Investigated were origin of the VA and its level of entrance into vertebral transverse foramen with notification of the sex and side of variation.
Results
The VA entered the C6 transverse process in 92.60% of specimens (413 out of 446 VA courses, 64.57% males and 28.03% females). Abnormal entrance of VA was observed in 7.40% of specimens (33 VA courses), with the level of entrance into the C3, C4, C5, or C7 transverse foramen at 0.22%, 2.24%, 4.72% and 0.22% respectively. Comparably, we observed that the overall variability of abnormal origin of VA was 3.14% (7 out of 223 subjects), which LVAs all arose from aortic arch (AA). Comparing subgroups of subjects with normal and abnormal origin, there was significance difference in the frequency of entrance variation in the level of transverse foramen.
Conclusion
Abnormal entrance and origin of VA were observed in 7.40% and 3.14% of cases, which can be accurately appeared by CTA.
CCM signaling complex (CSC) couples both classic and non-classic progesterone receptor signaling
Authors: Zhang Jun , Abou-Fadel Johnathan , Jiang Xiaoting , Grajeda Brian , Padarti Akhil , Ellis Cameron , Flores Esmeralda , O Alyssa-Marie Cailing-De La 
Published: April 2022

Abstract
Background: Breast cancer, the most commonly diagnosed cancer, remains the second leading cause of cancer death in the United States, and excessive Progesterone (PRG) or Mifepristone (MIF) exposure, including females under Hormone Replacement Therapy or taking hormonal contraceptives during their reproductive ages, may be at an increased risk for developing breast cancer. PRG exerts its cellular responses through signaling cascades involving classic, non-classic, or combined responses by binding to either classic nuclear PRG receptors (nPRs) or non-classic membrane PRG receptors (mPRs). Currently, the intricate balance and switch mechanisms between these two signaling cascades remain elusive. Three genes, CCM1-3, form the CCM signaling complex (CSC) which mediates multiple signaling cascades. Methods: Utilizing molecular cellular biology, Omics, and systems biology approaches, we analyzed the relationship among CSC, PRG, and nPRs/mPRs. Results: We discover that the CSC plays an essential role in coupling both classic and non-classic PRG signaling pathways by mediating crosstalk between them, forming the CmPn (CSC-mPRs-PRG-nPRs) signaling network. We found that mPR-specific PRG actions (PRG+Mifepristone) play an essential role in this CmPn network during breast cancer tumorigenesis; identified 4 categories of candidate biomarkers (9 intrinsic, 2 PRG-inducible, 1 PRG-repressive, 1 mPR-specific PRG-repressive, and 2 mPR-responsive) for Luminal-A breast cancers during tumorigenesis; and confirmed prognostic application of RPL13 and RPL38 as intrinsic biomarkers in Luminal-A breast cancers using a dual validation method. Conclusions: We discover that the CSC plays an essential role in the CmPn signaling network.
Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I
Authors: Chang Yin , Liu Liu , Zhang wei , Yang Qing-zhu , Yu Tian-fei , Jiang Xin-jie 
Published: April 2022

Abstract
Background
Mammalian intestinal microbiomes are necessary for antagonizing systemic viral infections. However, very few studies have identified whether poultry commensal bacteria play a crucial role in protecting against systemic viral infections. Nephropathogenic infectious bronchitis virus (IBV) is a pathogenic coronavirus that causes high morbidity and multiorgan infection tropism in chickens.
Results
In this study, we used broad-spectrum oral antibiotics (ABX) to treat specific pathogen free (SPF) chickens to deplete the microbiota before infection with nephropathogenic IBV to analyze the impact of microbiota on IBV infections in vivo. Depletion of the SPF chicken microbiota increases pathogenicity and viral burden following IBV infection. The gnotobiotic chicken infection model further demonstrated that intestinal microbes are resistant to nephropathogenic IBV infection. In addition, ABX-treated chickens showed a severe reduction in macrophage activation, impaired type I IFN production, and IFN-stimulated gene expression in peripheral blood mononuclear cells and the spleen. Lactobacillus isolated from SPF chickens could restore microbiota-depleted chicken macrophage activation and the IFNAR-dependent type I IFN response to limit IBV infection. Furthermore, exopolysaccharide metabolites of Lactobacillus spp. could induce IFN-β.
Conclusions
This study revealed the resistance mechanism of SPF chicken intestinal microbiota to nephropathogenic IBV infection, providing new ideas for preventing and controlling nephropathogenic IBV.
Ginsenoside Rh2 sensitizes the anti-cancer effects of sunitinib by inducing cell cycle arrest in renal cell carcinoma
Authors: Hwang Hyun Ji , Hong Seong Hwi , Moon Hong Sang , Yoon Young Eun , Park Sung Yul 
Published: April 2022

Abstract
Sunitinib, a VEGF blockade, is used to treat clear cell renal cell carcinoma (ccRCC). However, the anti-cancer treatment effects of sunitinib do not last long in ccRCC patients. Ginsenoside, a natural medicine extracted from ginseng, has been studied in cancer treatment and shown to have anti-tumor effects and low toxicity. We assessed cell viability and cell cycle analysis in ccRCC cell lines after treatment with ginsenoside and sunitinib. DNA damage was evaluated by measuring 8-OHdG levels and comet assay. ROS levels, reflecting cause of oxidative stress, were also measured. Ginsenoside significantly enhanced the inhibition of cell viability by sunitinib, a result that was also confirmed in the xenograft model. In cell cycle analysis, combination treatment of ginsenoside and sunitinib enhanced G2M arrest in comparison with single-treatment groups. In addition, DNA damage was increased by ginsenoside and sunitinib according to the comet assay, and the level of 8-OHdG, which reflects oxidative DNA damage, also increased. We verified that ginsenoside enhances the efficacy of sunitinib to inhibit the proliferation of ccRCC cells via induction of oxidative DNA damage. The combination therapy of sunitinib and ginsenoside is an effective new treatment strategy for RCC patients.