bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of the Relationships Between Optical and Physical Properties for Nanostructure TiO2 Thin Film
Authors: Haider Adawiya , Ali Ghalib , Haider Mohammed , Dheyab Amer , Thamir Amin 
Published: January 2022

Abstract
Pulsed Laser Deposition (PLD) technique was performed to deposit the pure Titanium oxide (TiO2) nanoparticles on the glass substrate of temp. (100 - 400°C), using the doubled frequency of Nd: YAG laser wavelength of 532nm at (10) Hz rate, 10 nanosecond duration pulses and a constant laser energy 800 mJ. The optical measurements obtained by UV-Vis transmittance on deposited TiO2 films indicate the highest transparency in the visible wavelength region with an average transmittance of 80%. Estimated the relationship between the refractive index of TiO2 thin films with substrate temperature was n = 2.49 at 400 oC. Moreover, the calculated empirical relation between the energy gap and refractive index have similar to the work results.
Association of a 20-lncRNA signature with mutation load of colon adenocarcinoma and immune microenvironment
Authors: Lin Yan , Li Qian , Liu Ziyu , Liang Rong , Ye Jiazhou , He Ziqin , Gao Xing , Zhang Jinyan , Luo Min , Li Yongqiang , Ge Lianying 
Published: January 2022

Abstract
Purpose
Tumor mutation burden (TMB) is an emerging biomarker for predicting immune checkpoint inhibitors (ICI). However, the role of TMB associated with long non-coding RNAs (lncRNAs) expression was not clear for preexisting immunity in colon adenocarcinoma (COAD).
Methods
The mutation data for COAD was downloaded from the Cancer Genome atlas (TCGA) database. A set of differentially expressed lncRNAs (DE lncRNAs) were identified between high or low TMB colon adenocarcinoma samples through differential expression analysis. The absolute shrinkage and selection operator (LASSO) method was used to define a subset of the DE lncRNAs as a “signature” for predicting TMB levels in the training cohort. After validating the signature on a test cohort, its potential correlations with the indicators of the efficacy of anti-immune checkpoint therapy were explored. Gene set variation analysis (GSVA) was carried out to explore the biological functions between high and low signature DE lncRNAs sample groups.
Results
A signature comprising 20 DE lncRNAs predicted TMB levels was identified by lasso method. And the model was with high accuracy in the training cohort (AUC=0.92) and test cohort (AUC=0.93). Moreover, the signature was also associated with T-effector and interferon-γ gene signature, expression of PDCD1, CD274, CTLA4, as well as abundance of TILs. Tumors receiving a low score for the 20-lncRNA signature received a high GSVA score for multiple immune-related pathways.
Conclusions
The 20-lncRNA signature can accurately predict TMB in colon adenocarcinoma and is associated with known indicators of immune checkpoint inhibitor efficacy and preexisting immunity.
The Global Publication Trend and Research Development of Nerve Block: A Visualisation Analysis Between 1997 and 2021
Authors: Dong Xiang , Zhao Pengcheng , Zhao Hui , Liu Jinxing , Chen Jie , Jiang Hong 
Published: January 2022

Abstract
Background: Nerve block is used as a clinical technique for relieving pain during the surgical period as well as in postoperative analgesia and chronic pain. Although great progress has been recently made regarding nerve block techniques, there is still room for further medical and technical improvement. Here, we analysed the trends in nerve block research and compared contributions from different countries, institutions, journals, and authors. Methods: We extracted all studies concerning nerve block published between 1997 and 2021 from the Web of Science database. Microsoft Excel and VOSviewer were employed to collect publication data, analyse publication trends, and visualize relevant results. Results: A total of 6,437 publications with 111,612 citations were identified between 1997 and 2021. The United States contributed the greatest number of publications (2010), whereas China ranked second (518). The Regional Anesthesia & Pain Medicine journal had published the most papers concerning nerve block. Harvard University and Mariano ER were the most productive institution and author, respectively, in the field. Keywords were categorized into five clusters: the association between nerve block and anaesthesia or analgesia, local anaesthetic drugs for nerve block, nerve block techniques, nerve block for postoperative analgesia, and nerve block for oral local anaesthesia. Management-related research was a heavily researched topic in the field. Conclusions: We concluded that the United States contributed the most studies, and developing countries, led by China, are also encouraging study in this area. The exploration of new drugs in the fields of nerve block, multi-modal analgesia after surgery, treatment of chronic pain, analgesia for thoracic and abdominal surgeries, and analgesic interventions in oral therapy is considered as a potential focus for future research.
Identification of Some Dietary Flavonoids as Potential Inhibitors of TMPRSS2 Through Protein-ligand Interaction Studies and Binding Free Energy Calculations
Authors: Varughese Jibin K , J Kavitha , S Sindhu K , Francis Dhiya , L Joseph Libin K , G ABI T 
Published: January 2022

Abstract
The alarming increase in COVID-19 cases and deaths calls for an urgent cost-effective pharmacological approach. Here, we examine the inhibitory activity of a group of dietary bioactive flavonoids against the human protease TMPRSS2, which plays a major role in SARS CoV-2 viral entry. After the molecular docking studies of a large number of flavonoids, four compounds with high binding scores were selected and studied in detail. The binding affinities of these four ligands, Amentoflavone, Narirutin, Eriocitrin, and Naringin, at the active site of TMPRSS2 target were investigated using MD simulations followed by MM-PBSA binding energy calculations. From the studies, a number of significant hydrophobic and hydrogen bonding interactions between the ligands and binding site amino residues of TMPRSS2 are identified which showcase their excellent inhibitory activity against TMPRSS2. Among these ligands, Amentoflavone and Narirutin showed MM-PBSA binding energy values of -155.48 and -138.13 kJ/mol respectively. Our previous studies of the inhibitory activity of these compounds against main protease of SARS-COV2 and the present study on TMPRSS2 strongly highlighted that Amentoflavone and Naringin can exhibit promising multi-target activity against SARS-CoV-2. Moreover, due to their wide availability, no side effects and low cost, these compounds could be recommended as dietary supplements for COVID patients or for the development of SARS-CoV-2 treatments.
Effects of Resilience on Impulsivity, Cognition and Depression during Protracted Withdrawal among Chinese Male Methamphetamine Users
Authors: Zhou Siyao , Ran Wang , Peng Chenhui , Tong Siyu , Hong Lan , Cai Fangfang , Jin Wei , Jiang Yile , Li Mengjia , Wang Xuanping , Luo Mengdan , He He , Wang Wei , Zhao Ke 
Published: January 2022

Abstract
Background

Methamphetamine (METH) dependence is a complex and dynamic public health problem. Long-term abuse of METH can increase numerous risks of mental and physical problems. Currently, the methods to reduce METH dependence and improve the withdrawal symptoms are limited and ineffective. Resilience is regarded as an individuals’ protective moderator against stress and may play a role in the stress-related mental diseases.
Methods

One hundred and twelve males with METH dependence were consecutively recruited from Huanglong Compulsory Isolated Detoxification Center between 2019 and 2021. The Connor-Davidson Resilience Scale (CD-RISC), Self-rating depression scale (SDS), Self-rating anxiety scale (SAS), Barratt Impulsiveness Scale-11(BIS-11), and the Repeatable Battery for the Assessment of Neuropsychological Status (Rbans) were used to evaluate resilience, depression, anxiety, impulsivity, and cognition respectively.
Results

The results showed that high and medium resilience groups showed lower SDS scores than the low resilience group (p<0.05), with high resilience groups showing lower scores using SAS (p<0.05). The high resilience group demonstrated lower cognitive impulsiveness, non-planning impulsiveness, and BIS-11 scores than the low resilience group (all p <0.05). Additionally, compared with the low resilience group, attention was better in the medium resilience group, while delayed memory was better in the high resilience group (both p<0.05). The total scores of Rbans were also higher in the medium and high resilience groups than low resilience group (both p<0.05).
Conclusions

This study confirmed a correlation between resilience and impulsivity, cognitive function, and depression. It may suggest a potential role of resilience for individuals during protracted METH withdrawal.
Two Shades of Grey: Effect of Temperature on Seed Germination of the Escaping Ornamental Species Lychnis Coronaria and Stachys Byzantina
Authors: Vojík Martin , Kadlecová Martina , Kutlvašr Josef , Pergl Jan , Bímová Kateřina Berchová 
Published: January 2022

Abstract
This paper describes germination and cytological variability in two popular ornamental and potentially invasive species, lamb’s ear Stachys byzantina and rose campion Lychnis coronaria. Both xerophytic species have the potential to invade natural habitats across Europe and create viable naturalised populations, with subsequent impacts on native vegetation. To assess the species’ invasiveness, seeds were collected from naturalised populations and germination rate recorded under different temperature regimes. Flow cytometry, used to record cytological variability, indicated that all populations of both species were cytologically homogeneous. Germination success, a key spreading factor in both species, was significantly influenced by temperature, with final germination of L. coronaria being extremely high at temperatures > 15/10 °C (98.5%) and extremely low at temperatures < 10/5 °C (2.9%). In comparison, final germination in S. byzantina highest at 22/15 °C (55.6%), reducing to 40.3% at 15/10 °C and just 0.3% at temperatures < 10/5 °C. No significant differences in germination rate were observed between escaping and non-escaping populations, though there were differences between particular populations. Our results indicate germination temperature limits between species consistent with sizes of primary distribution and distance between primary and secondary distribution borders. However, the observed germination rates allow for successful generative reproduction of both species over their secondary distribution areas, suggesting that these species are likely to become invasive species of European grasslands soon.
Risk Analysis of Tunnel Water and Mud Inrush Using Interpretive Structural Modeling and Fault Tree
Authors: Han Min , Xia Teng , Su Maoxin , Xue Yiguo 
Published: January 2022

Abstract
Water and mud inrush is a common geological hazard in tunnel construction. Risk analysis of tunnel water and mud inrush has always been an important subject. In order to avoid the geological hazard, this paper presents a risk analysis model of tunnel water and mud inrush. The model combines the interpretive structural modeling method (ISM) and fault tree analysis (FTA). Relying on the Qinyu tunnel in the Weiwu expressway project, water and mud inrush risk factors are obtained by using ISM. Fundamental risk factors include formation lithology, attitude of stratum, strata combination, topography and geomorphology, geological structure and weather. ISM core risk factors are used as FTA basic events. Fuzzy importance of FTA basic events is obtained by using fuzzy interval calculation. The results show that geological structure is the primary risk factor causing Qinyu tunnel water and mud inrush. The model achieves qualitative and quantitative analysis of tunnel water and mud inrush. It accurately determines the main factors affecting the tunnel water and mud inrush, which is conducive to accident prevention.
Combination Therapy Versus Monotherapy In The Treatment Of Stenotrophomonas Maltophilia Infections: A Systematic Review And Meta-Analysis
Authors: Prawang Abhisit , Chanjamlong Naphatsawan , Rungwara Woranattha , Santimaleeworagun Wichai , Paiboonvong Taniya , Manapattanasatein Thidarat , Pitirattanaworranat Prompiriya , Kitseree Pongsakorn , Kanchanasurakit Sukrit 
Published: January 2022

Abstract
Background: Stenotrophomonas maltophilia is a multidrug-resistant bacteria that is difficult to treat in hospitals around the world. It has become a public health issue, as well as being linked to a high mortality rate. Several studies have shown a variety of treatment and clinical outcomes; however, the efficacy of combination therapy remains limited. Therefore, the purpose of this study is to investigate the effect of monotherapy and combination therapy for S. maltophilia infections on mortality outcome.Methods: We performed a systematic review and meta-analysis of combination therapy versus monotherapy in the treatment of S. maltophilia infections on mortality as a clinical outcome. Electronic databases, including Cochrane Library, PubMed, EMBASE, ClinicalTrials.gov, Scopus, and OpenGrey, were systematically searched from the inception of the database until September 3, 2021. Results: Of which 6,524 articles identified, a total of 13 studies and 2 cohort studies were included for systematic review of combination therapy and meta-analysis, respectively. The systematic review of combination antimicrobial therapy had been showed clinically desirable outcome on mortality in S. maltopholia infection, especially in complex or severe infection. In the fixed-effects meta-analysis of the cohort study, monotherapy was surprisingly shown to have statistically significant effects on the decreased risk of mortality (hazard ratio 1.42; 95% confidence interval, 1.04-1.94). Conclusions: Our results found that the combination antimicrobial therapy had been showed clinically desirable outcome on mortality in S. maltopholia infection and monotherapy has a trend toward improved better outcome than combination therapy on mortality for the treatment of S. maltophilia infections. A longitudinal study that further explores this association is warranted. Trial registration: This study was registered with the trial registration number ID: 210843 under the international prospective register of systematic reviews (PROSPERO: www.crd.york.ac.uk/PROSPERO).
An Immunological Axis Involving Interleukin 1β and Leucine-Rich-α2-Glycoprotein Reflects Therapeutic Response of Children with Kawasaki Disease: Implications from the KAWAKINRA Trial
Authors: Kessel Christoph , Kone-Paut Isabelle , Tellier Stephanie , Belot Alexandre , Masjosthusmann Katja , Wittkowski Helmut , Fuehner Sabrina , Rossi-Semerano Linda , Dusser Perrine , Marie Isabelle , Boukhedouni Nadja , Agostini Helène , Piedvache Céline , Foell Dirk 
Published: January 2022

Abstract
Purpose

A recent phase II open-label study of the interleukin 1 (IL-1) receptor antagonist (IL-1Ra) anakinra in treating IVIG-resistant Kawasaki Disease (KD) patients reported promising results. Here, we aimed to characterize the immunological impact of IL-1 blockade in this unique study population.
Methods

Patients’ and control sera and supernatants of cells (whole blood, neutrophils, coronary artery endothelial cells) stimulated with recombinant IL-1β were analyzed for single or multiple marker (n=22) expression by ELISA or multiplexed bead array assay. Data were analyzed using unsupervised hierarchical clustering, multiple correlation and multi-comparison statistics and were compared to retrospective analyses of KD transcriptomics.
Results

Inflammation in IVIG-resistant KD (n=16) is hallmarked by over-expression of innate immune mediators (particularly IL-6>CXCL10>S100A12>IL-1Ra). Those as well as levels of immune or endothelial cell activation markers (sICAM-1, sVCAM-1) declined most significantly in course of anakinra treatment. Prior as well as following IL-1R blockade, over-expression of leucine-rich-α2-glycoprotein 1 (LRG1) associated best with remnant inflammatory activity and the necessity to escalate anakinra dosage and separated inflammatory KD patients from sJIA-MAS (n=13) and MIS-C (n=4). Protein as well as retrospective gene expression analyses indicated tight association of LRG1 with IL-1β signaling and neutrophilia, while particularly neutrophil stimulation with recombinant IL-1β resulted in concentration-dependent LRG1 release.
Conclusion

Our study identifies LRG1 as known trigger of endothelial activation and cardiac re-modelling to associate with IL-1β signaling in KD. Besides a potential patho-mechanistic implication of these findings, our data suggest blood leukocyte and neutrophil counts to best predict response to IL-1Ra treatment in IVIG-resistant KD.
The green, ultrafine cellulose-based porous nanofibrous membranes for efficient heavy metal ion removal through incorporation of chitosan by various electrospinning ways
Authors: Li Qiushi , Su Ganmao , Luo Ronggang , Du Guanben , Xie Linkun , Tang Zhengguan , Yan Jinsong , Zhou Juying , Wang Siqun , Xu Kaimeng 
Published: January 2022

Abstract
The rapid global industrialization worsens the contamination of heavy metals in aquatic ecosystems on the earth. In this study, the green, ultrafine cellulose-based porous nanofibrous membranes for efficient heavy metal removal through incorporation of chitosan by the conventional and core-shell electrospinning ways were firstly obtained. The relations among parameters of electrospun solution, micro-morphology and porosity for nanofibers, the variation of chemical active sites and adsorption performance of biocomposite nanofibrous membranes for conventional and core-shell electrospinning as well as the adsorption effect factors of copper ions including initial concentration, pH of solution and interaction time were comprehensively investigated. The results show that the average diameter for conventional and core-shell ultrafine nanofibers at 50% chitosan and 30% chitosan loading can achieve 56.22 nm and 37.28 nm, respectively. The core-shell cellulose acetate/chitosan (CA/CS) biocomposite nanofibrous membranes induced the surface aggregation of copper ions to impede the further adsorption. The more uniform distribution for chemical adsorption sites can be obtained by the conventional single-nozzle electrospinning than by the core-shell one, which promotes the adsorption performance of copper ions and decreases the surface shrinkage of nanofibrous membranes during adsorption. The 30% CS conventional nanofibrous membranes at the pH=5 aqueous solution showed the optimum adsorption capacity of copper ions (86.4 mg/g). The smart combination of renewable biomass with effective chemical adsorptive sites, the electrospinning technology with interwoven porous structure and the adsorption method with low cost and facile operation shows a promising prospect for water treatment.
Genetic Diversity and Structure of Core Collection of Huangqi (Astragalus) Developed by Genomic Simple Sequence Repeat Markers
Authors: Gong Fanshu , Geng Yaping , Zhang Pengfei , Zhang Feng , Fan Xinfeng , Liu Yaling 
Published: January 2022

Abstract
Huangqi (Astragalus) is a versatile herb that possesses several therapeutic effects against a variety of diseases, especially lung diseases. The aim of this study was to establish a core collection of Astragalus germplasm resources based on molecular 10 SSR markers. Based on 380 samples of Astragalus collected from different areas, five different methods were utilized to construct the core collection of Astragalus, including PowerCore-based M strategy, CoreFinder-based M strategy, Core Hunter-based stepwise sampling, PowerMarker-based simulated annealing algorithm based on allele maximization, and PowerMarker-based simulated annealing algorithm based on maximizing genetic diversity. Of the constructed Astragalus core collections, the CoreFinder-based M strategy was found to be the most suitable approach as it reserved all the alleles and most of the genetic diversity parameters were higher than those of the initial collection. Additional analyses demonstrated that the genetic diversity of the core collection matched the properties of the initial collection. Further, the phylogenetic trees indicated that the population structure of the core collection was similar to that of the initial collection. In addition, our results showed that the optimal grouping value of K was 2. The construction of a core collection is beneficial for the understanding, management, and utilization of Astragalus. Moreover, this study will act as a valuable reference for constructing core collections for other plants or fungi.
­­Access to Health Insurance and Treatment Utilization for Opioid Use Disorder: Evidence From State Medicaid Expansions
Authors: Datta Anusua , Oglesby Willie , George Brandon 
Published: January 2022

Abstract
Background. Medicaid is a major payer of substance use disorder treatment, yet the impact of Medicaid expansion on the opioid epidemic has not been sufficiently quantified. This study exploits state-level differences in Medicaid expansion to assess the impact of access to health insurance on treatment utilization for opioid use disorder (OUD) for adults in need.Method. We use admissions data from Treatment Episode Data Sets (TEDS) to fit a multivariate difference-in-difference model, with non-expanding states as controls, adjusting for age, gender, race/ethnicity, education, and other state-level factors. Results. Medicaid expansion led to substantial gain in OUD treatment utilization. Admissions for substance use disorder among Medicaid beneficiaries increased by 20-33 percentage points in expansion states. Admissions were significantly higher for the newly eligible Medicaid beneficiaries in the 30-34 age group. We also see an increase in treatment admissions for opioid and heroin use among the elderly over the age of 55. Uninsurance rates show a commensurate decline in the expansion states. Finally, we do not find strong evidence of crowding-out of private insurance. Conclusions. Overall, our findings suggest that Medicaid expansions had a positive impact on the financing and utilization of opioid use treatment.
Multi-Block Data Integration Analysis for Identifying and Validating Targeted N-Glycans as Biomarkers for Type II Diabetes Mellitus
Authors: Adua Eric , Yamoah Ebenezer Afrifa , Peprah-Yamoah Emmanuel , Anto Enoch Odame , Acheampong Emmanuel , Mensah Kwaafo Awuah , Wang Wei 
Published: January 2022

Abstract
Plasma N-glycan profiles have been shown to be defective in type II diabetes Mellitus (T2DM) and holds a promise to discovering biomarkers. The study comprised 232 T2DM patients and 219 healthy individuals. N-glycans were analysed by high-performance liquid chromatography. Principal component analysis (PCA), discriminatory analysis and agglomerative hierarchical cluster analysis (HCA) were performed. N-glycan groups (GPs 34, 32, 26, 31, 36 and 30) were significantly expressed in T2DM in component 1 and GPs 38 and 20 were related to T2DM in component 2. Four clusters based on the correlation of the expressive signatures of the 39 N-glycans across T2DM and controls. Cluster A, B, C and D had 16, 16, 4 and 3 N-glycans respectively, of which 11, 8, 1 and 1 were found to express differently between controls and T2DM in a univariate analysis P. Multi-block analysis revealed that trigalactosylated (G3), triantennary (TRIA), high branching (HB) and trisialylated (S3) expressed significantly highly in T2DM than healthy controls. A bipartite relevance network revealed that HB, monogalactosylated (G1) and G3 were central in the network and observed more connections, highlighting their importance in discriminating between T2DM and healthy controls. Investigation of these N-glycans can enhance the understanding of T2DM.