bluuebunny/crossref_metadata_2025_split huggingface dataset:
Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates
Authors: Aurich Konstanze , Wesche Jan , Ulbricht Martin , Otto Oliver , Greinacher Andreas , Palankar Raghavendra 
Published: April 2022

AbstractCold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca2+release from storage organelles and higher activity of Ca2+pumps at temperatures < 15 °C triggers cytoskeleton changes. This is suppressed by Mg2+addition, avoiding a shift in Ca2+hemostasis and cytoskeletal alterations. We report on the impact of 2–10 mM Mg2+on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 °C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affected by storage at 4 °C and preserved by Mg2+addition ≥ 4 mM Mg2+(mean ± SD of median deformation 4 °C vs. 4 °C + 10 mM Mg2+0.073 ± 0.021 vs. 0.118 ± 0.023,p < 0.01; n = 6, day 7). These results were confirmed by immunofluorescence microscopy, showing that Mg2+ ≥ 4 mM prevents 4 °C storage induced cytoskeletal structure lesion. Standard in vitro platelet function tests showed minor differences between RT and cold-stored platelets. Hypotonic shock response was not significantly different between RT stored (56.38 ± 29.36%) and cold-stored platelets with (55.22 ± 11.16%) or without magnesium (45.65 ± 11.59%; p = 0.042, all n = 6, day 1). CD62P expression and platelet aggregation response were similar between RT and 4 °C stored platelets, with minor changes in the presence of higher Mg2+concentrations. In conclusion, increasing Mg2+up to 10 mM in PAS counteracts 4 °C storage lesions in platelets, maintains platelet cytoskeletal integrity and biomechanical properties comparable to RT stored platelets.
Predictors of impaired SARS-CoV-2 immunity in healthcare workers after vaccination with BNT162b2
Authors: Bertram Sebastian , Blazquez-Navarro Arturo , Seidel Maximilian , Hölzer Bodo , Seibert Felix S. , Doevelaar Adrian , Rohn Benjamin , Zgoura Panagiota , Witte-Lack Alexandra , Skrzypczyk Sarah , Scholten David , Kisters Klaus , Babel Nina , Westhoff Timm H. 
Published: April 2022

AbstractHealthcare workers are at substantially increased risk for infection with SARS-CoV-2. Successful vaccination constitutes a crucial prerequisite to protect this group during the pandemic. Since post vaccination antibody monitoring is not standard of care in all healthcare institutions, data on risk factors of impaired vaccine induced immune response are urgently required. Moreover, there are no data on cellular immune responses in humoral low responders so far. Anti-SARS-CoV-2 spike IgG was assessed after vaccination with BNT162b2 in 1386 employees of three hospitals of a German healthcare provider. Concentrations were compared to those of 45 convalescent employees. Vaccine-induced cellular immunity was measured in employees with reduced humoral response by assessment of frequencies of SARS-CoV-2-reactive CD4+ and CD8+ T cell. Anti-SARS-CoV-2 spike IgG were detected in 99.9% of 1386 healthcare workers after completed vaccination. The median antibody concentration was significantly higher after vaccination than after infection with SARS-CoV-2 (p = 0.0001). 10 subjects (0.7%) generated an IgG concentration < 100 IU/ml, and only two persons (0.1%, solid organ recipients) did not produce detectable antibodies at all. T cell responses of those subjects with submaximal or lacking humoral response were comparable to employees with maximal antibody titers. 50% of those individuals with impaired or lacking humoral immune response were on immunosuppression. Vaccination to SARS-CoV-2 with BNT162b2 is very effective in healthcare workers yielding a seroconversion rate of 99.9%. Immunosuppression is the most important risk factor of an impaired immune response. There was no case of vaccination failure without immunosuppression. Thus, post vaccination antibody monitoring is highly recommendable in those employees with immunosuppression.
Seasonal-to-decadal prediction of El Niño–Southern Oscillation and Pacific Decadal Oscillation
Authors: Choi Jung , Son Seok-Woo 
Published: April 2022

AbstractThe growing demand for skillful near-term climate prediction encourages an improved prediction of low-frequency sea surface temperature (SST) variabilities such as the El Niño-Southern Oscillation (ENSO) and Pacific Decadal Oscillation (PDO). This study assesses their seasonal-to-decadal prediction skills using large ensembles of the Coupled Model Intercomparison Project phases 5 and 6 retrospective decadal predictions. A multi-model ensemble reforecast successfully predicts ENSO over a year in advance. While its seasonal prediction skill in the following spring and summer is achieved by multi-model ensemble averaging of relatively smaller ensemble members, the multi-year prediction of winter ENSO needs a larger ensemble size. The PDO is significantly predicted at a lead time of five-to-nine years but such a long-lead prediction is sourced from external radiative forcing instead of initialization, as evidenced from uninitialized historical simulations. The effect of model initialization lasts only two years. These results confirm that both the model initialization and the proper estimate of near-term radiative forcing are required to improve the seasonal-to-decadal prediction in the Pacific Basin.
Diesel engine diagnosis based on entropy of vibration signals and machine learning techniques
Authors: Hernández Juan Camilo Mejía , Madrid Federico Gutiérrez , Quintero Héctor Fabio , Alzate Juan David Ramírez 
Published: April 2022

AbstractCompression‐ignition (CI) engines, aka diesel engines, are responsible for an essential percentage of the world‐polluting emissions. Moreover, bearings installed in industrial machinery constitute the most common failure affecting global energy consumption. Since industries’ energy demand has a growing tendency, efficient maintenance is a must. Maintenance requires a fast and accurate diagnosis, commonly based on an intrusive or expensive sensor to capture monitoring signals, i.e. pressure, emissions, temperature, fuel consumption and rotational speed. Here, a vibration signal‐based approach is introduced to combustion engines and bearings diagnosis. Namely, a multi‐scale permutation entropy (MPE)‐based feature extraction is conducted within a variability‐based relevance analysis (VRA) stage to feed a straightforward classifier, the K‐nearest neighbours (KNN). Accuracy was validated using a signals’ database from a single‐cylinder engine under multiple work conditions. Also, the methodology is compared through classification accuracy of a widely known bearing vibration signal database obtaining an outstanding performance.
A novel rapid visual detection assay for Toxoplasma gondii combining recombinase-aided amplification and lateral flow dipstick coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD)
Authors: Zhao Jinhong , Li Yuanyuan , Xue Qiqi , Zhu Zhiwei , Zou Minghui , Fang Fang 
Published: April 2022

Toxoplasmosis, a parasitic disease resulting from Toxoplasma gondii infection, remains prevalent worldwide, and causes great harm to immunodepressed patients, pregnant women and newborns. Although various molecular approaches to detect T. gondii infection are available, they are either costly or technically complex. This study aimed at developing a rapid visual detection assay using recombinase-aided amplification (RAA) and lateral flow dipstick (LFD) coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD) to detect T. gondii. The RAA-Cas13a-LFD assay was performed in an incubator block at 37 °C within 2 h, and the amplification results were visualized and determined through LFD by the naked eye. The detection limit was 1 × 10−6 ng/μL by our developed RAA-Cas13a-LFD protocol, 100-fold higher than that by qPCR assay (1 × 10−8 ng/μL). No cross-reaction occurred either with the DNA of human blood or Ascaris lumbricoides, Digramma interrupta, Entamoeba coli, Fasciola gigantica, Plasmodium vivax, Schistosoma japonicum, Taenia solium, and Trichinella spiralis, and the positive rate by RAA-Cas13a-LFD assay was identical to that by qPCR assay (1.50% vs. 1.50%) in detecting T. gondii infection in the unknown blood samples obtained from clinical settings. Our findings demonstrate that this RAA-Cas13a-LFD assay is not only rapid, sensitive, and specific and allows direct visualization by the naked eye, but also eliminates sophisticated and costly equipment. More importantly, this technique can be applied to on-site surveillance of T. gondii.
Clinical safety and outcomes of glucagon use during endoscopic retrograde cholangiopancreatography (ERCP)
Authors: Perisetti Abhilash , Goyal Hemant , Sharma Neil 
Published: April 2022

Abstract
Background and study aims Injectable glucagon enables easier biliary cannulation by inhibiting gastrointestinal motility and decreasing the frequency and amplitude of phasic activity of the sphincter of Oddi during endoscopic retrograde cholangiopancreatography (ERCP). Data about the safety profile of glucagon use and patient clinical outcomes are scarce.
Patients and methods We used a federated cloud-based network research database, TriNetX, comprising 92 US healthcare organizations to find adult patients undergoing ERCP with glucagon use (Group A) vs. without using glucagon (Group B) from August 1, 2010, to August 1, 2021. The primary outcomes were rates of gastrointestinal bleeding, gastrointestinal perforation, post-ERCP pancreatitis, inpatient hospitalizations, and 30-day overall mortality measured after 1:1 propensity matching of the groups based on the baseline demographics and comorbidities.
Results There were 9,008 patients in Group A compared to 256,597 in Group B. After matching, Group A patients had lower rates of gastrointestinal bleeding (risk ratio [RR], 0.68; CI, 0.52–0.86), post-ERCP pancreatitis (RR, 0.64; CI, 0.58–71), inpatient hospitalization (RR 0.34; CI:0.32 to 0.36) and overall mortality (RR, 0.81; CI, 0.66–0.99). The rates of gastrointestinal perforation (RR, 0.64; CI: 0.34 to 1.19), hyperkalemia (RR, 0.83; CI, 0.64–1.09) and hyperglycemia (RR, 0.65; CI, 0.41–1.03) did not differ between the two groups.
Discussion Glucagon use during ERCP was associated with low rates of gastrointestinal bleeding, post-ERCP pancreatitis, inpatient hospitalization, and overall mortality. Moreover, the rates of hyperkalemia and hyperglycemia did not differ between the two groups even after matching for diabetes, indomethacin use, obesity, and chronic kidney disease.
Usefulness of optical enhancement endoscopy combined with magnification to improve detection of intestinal metaplasia in the stomach
Authors: Sobrino-Cossío Sergio , Teramoto-Matsubara Oscar , Emura Fabian , Araya Raúl , Arantes Vítor , Galvis-García Elymir S. , Meza-Caballero Marisi , García-Aguilar Blanca Sinahi , Reding-Bernal Arturo , Uedo Noriya 
Published: April 2022

Abstract
Background and study aims The light blue crest observed in narrow band imaging endoscopy has high diagnostic accuracy for diagnosis of gastric intestinal metaplasia (GIM). The objective of this prospective study was to evaluate the diagnostic accuracy of magnifying i-scan optical enhancement (OE) imaging for diagnosing the LBC sign in patients with different levels of risk for gastric cancer in a Mexican clinical practice.
Patients and methods Patients with a history of peptic ulcer and symptoms of dyspepsia or gastroesophageal reflux disease were enrolled. Diagnosis of GIM was made at the predetermined anatomical location and white light endoscopy and i-scan OE Mode 1 were captured at the two predetermined biopsy sites (antrum and pyloric regions).
Results A total of 328 patients were enrolled in this study. Overall GIM prevalence was 33.8 %. The GIM distribution was 95.4 % in the antrum and 40.5 % in the corpus. According to the Operative Link on Gastritis/Intestinal-Metaplasia Assessment staging system, only two patients (1.9 %) were classified with high-risk stage disease. Sensitivity, specificity, positive​ and negative predictive values, positive and negative likelihood ratios, and accuracy of both methods (95 % C. I.) were 0.50 (0.41–0.60), 0.55 (0.48–0.62), 0.36 (0.31–0.42), 0.68 (0.63–0.73), 1.12 (0.9–1.4), 0.9 (0.7–1.1), and 0.53 (0.43–0.60) for WLE, and 0.96 (0.90–0.99), 0.91 (0.86–0.94), 0.84 (0.78–0.89), 0.98 (0.94–0.99), 10.4 (6.8–16), 0.05 (0.02–0.12), and 0.93 (0.89–0.95), respectively. The kappa concordance was 0.67 and the reliability coefficient was 0.7407 for interobserver variability.
Conclusions Our study demonstrated the high performance of magnifying i-scan OE imaging for endoscopic diagnosis of GIM in Mexican patients.
Effects of a Multicomponent Exercise Training Program on Balance
Following Bariatric Surgery
Authors: Diniz-Sousa Florêncio , Granja Tiago , Boppre Giorjines , Veras Lucas , Devezas Vítor , Santos-Sousa Hugo , Preto John , Machado Leandro , Vilas Boas Joao Paulo , Oliveira Jose , Fonseca Hélder 
Published: April 2022

AbstractPatients who undergo bariatric surgery (BS) have an increased risk of falls. Our
aim was to determine if a multicomponent exercise intervention after BS improves
balance. Eighty-four patients with obesity enrolled for BS were recruited and 1
month after BS randomly allocated to a control (CG; standard medical care) or
exercise group (EG; exercise plus standard medical care) consisting of a
supervised multicomponent training program (3d/week; 75
min/session; 5 months). Anthropometry, lower limb muscle strength
(isokinetic dynamometer), vitamin D (ELISA) and balance in bipedal stance (force
platform) were assessed pre-BS, 1 month and 6 months post-BS. One month post-BS,
significant balance improvements were observed, namely in antero-posterior
center of gravity (CoG) displacement and velocity, and medio-lateral and total
CoG velocity. Between 1- and 6-months post-BS, improvements in balance were
observed only in the EG, with a significant treatment effect on CoG displacement
area and antero-posterior CoG displacement. No significant differences were
observed between EG and CG over time in any of the anthropometric, muscle
strength, and vitamin D variables assayed. In conclusion, a multicomponent
exercise intervention program improves some balance parameters in patients with
severe obesity following BS and therefore should be part of post-BS follow-up
care as a potential strategy to reduce falls and associated injuries.
Kutane Sarkome – nicht alle sind wirklich selten, die meisten „low risk“
Authors: Vogt Thomas 
Published: April 2022

ZusammenfassungDie biologische und morphologische Vielfalt der Weichteilmalignome, der Sarkome, ist enorm.Diese Übersicht fokussiert deshalb auf die 5 am häufigsten vorkommenden Weichteilmalignome. Betrachtet werden soll dabei, was der Kliniker wissen muss, was neu ist und welche Fallstricke es gibt. Ziel dieses Artikels ist es dabei, eine Übersicht zu geben sowie die Konzeptbildung darüber, womit man es im Wesentlichen zu tun hat.
Digestive perforations related to endoscopy procedures: a local management charter based on local evidence and experts’ opinion
Authors: Bertrand Gaspard , Rivory Jérôme , Robert Maud , Saurin Jean-Christophe , Pelascini Élise , Monneuse Olivier , Gruner Laurent , Poncet Gilles , Valette Pierre-Jean , Gimonet Hélène , Rostain Florian , Ber Charles-Éric , Bouffard Yves , Boibieux André , Ciochina Marina , Landel Verena , Boyer Hélène , Jacques Jérémie , Ponchon Thierry , Pioche Mathieu 
Published: April 2022

Abstract
Background and study aims Perforations are a known adverse event of endoscopy procedures; a proposal for appropriate management should be available in each center as recommended by the European Society of Gastrointestinal Endoscopy. The objective of this study was to establish a charter for the management of endoscopic perforations, based on local evidence.
Patients and methods Patients were included if they experienced partial or complete perforation during an endoscopic procedure between 2008 and 2018 (retrospectively until 2016, then prospectively). Perforations (size, location, closure) and management (imagery, antibiotics, surgery) were analyzed. Using these results, a panel of experts was asked to propose a consensual management charter.
Results A total of 105 patients were included. Perforations occurred mainly during therapeutic procedures (91, 86.7%). Of the perforations, 78 (74.3 %) were diagnosed immediately and managed during the procedure; 69 of 78 (88.5 %) were successfully closed. Closures were more effective during therapeutic procedures (60 of 66, 90.9 %) than during diagnostic procedures (9 of 12, 75.0 %, P = 0.06). Endoscopic closure was effective for 37 of 38 perforations (97.4 %) < 0.5 cm, and for 26 of 34 perforations (76.5 %) ≥ 0.5 cm (P < 0.05). For perforations < 0.5 cm, systematic computed tomography (CT) scan, antibiotics, or surgical evaluation did not improve the outcome. Four of 105 deaths (3.8 %) occurred after perforation, one of which was attributable to the perforation itself.
Conclusions Detection and closure of perforations during endoscopic procedure had a better outcome compared to delayed perforations; perforations < 0.5 cm had a very good prognosis and CT scan, surgeon evaluation, or antibiotics are probably not necessary when the endoscopic closure is confidently performed. This work led to proposal of a local management charter.
Efficiency of an endoscopic resection strategy for management of submucosal tumors < 20 mm in the upper gastrointestinal tract
Authors: Caillol Fabrice , Meunier Elise , Zemmour Christophe , Ratone Jean-Philippe , Guiramand Jerome , Hoibian Solene , Dahel Yanis , Poizat Flora , Giovannini Marc 
Published: April 2022

Abstract
Background and study aims The histologic diagnosis of submucosal tumors (SMTs) < 20 mm is challenging. Monitoring is the main option offered, but compliance is debatable. Endoscopic resection (ER) of malignant SMTs or those with an uncertain diagnosis is an alternative that has already been reported about and proposed in our center. The aims of this study were to confirm the safety of this resection strategy and to perform long-term follow-up of malignant SMTs after resection.
Patients and methods All patients who underwent ER for SMTs < 2 cm in a single center between 2007 and 2019 were included retrospectively. Patients were classified into two groups according to the need for postresection follow-up: benign SMTs (B-SMTs) and follow-up SMTs (FU-SMTs).
Results One hundred and one patients were included. The mean tumor size was 16.7 mm. In total, 92 of 101 SMTs had an uncertain diagnosis. Macroscopic resection was completed for 95 SMTs (93.1 %), with en bloc resection in 94 (92.1%). The morbidity rate was 3 %, with no mortality. A total of 84 of 101 SMTs (84 %) were B-SMTs and did not need monitoring, and 17 SMTs (19.7 %) were FU-SMTs (8 gastrointestinal stromal tumors, 6 neuroendocrine tumors, and 3 others). No relapse was reported in the FU-SMT group, with a median follow-up duration of 33 months [4–127] (61 months [17–127] for the gastrointestinal stroma tumor group).
Conclusions The study results suggest ER is a potentially reliable and effective strategy for upper gastrointestinal tract SMTs < 20 mm. Although the strategy needs further validation in advanced care units, it could eliminate the need for long-term monitoring, therefore targeting such follow-up efforts to patients with FU-SMTs.
Endoscopy-induced anterior cutaneous nerve entrapment syndrome: a case series
Authors: Wolfhagen Frank H.J. 
Published: April 2022

Abstract
Background and study aims Anterior cutaneous nerve entrapment syndrome (ACNES) is a common but frequently overlooked disorder. Here we report on a series of patients with ACNES following endoscopy.
Patients and methods This case series included consecutive patients with localized abdominal pain following an endoscopic procedure that was consistent with ACNES who presented to the author’s Gastroenterology Outpatient Clinic from February 2019 to February 2021.
Results Six patients presented with complaints compatible with ACNES. All of them were successfully managed with local injection therapy (n = 5) or pulsed radiofrequency (PRF) (n = 1).
Conclusions It appears that ACNES can be induced by endoscopy. Early recognition is important to avoid unnecessary diagnostics and delayed pain relief in patients. Most patients can be managed with local injection therapy.
Cephalic Vein Aneurysm in the Distal Forearm Managed with Sclerotherapy—A Rare Case Report and Literature Review
Authors: Mahajan Ashima , Fazal Syed T. , Mehta Sanjay , Kataria Narender S. , Nanda Vipul 
Published: April 2022

AbstractVenous aneurysm of upper extremities is rare entity. We report a rare case of large cephalic vein (CV) aneurysm in a 41-year-old man who presented as slowly growing painless swelling at distal forearm along the lateral aspect. Duplex ultrasound showed aneurysm originating from the anterior wall of the CV with venous waveform pattern on Doppler. It was managed successfully with percutaneous sclerotherapy.
Effects of Electrode Location on Speech Recognition with the Nucleus-22 Cochlear Implant
Authors: Friesen Lendra M. , Shannon Robert V. , Slattery William H. 
Published: April 2022

AbstractSpeech recognition performance was measured as a function of electrode in two experiments with the Nucleus-22 cochlear implant using 4–electrode SPEAK speech processors. In experiment 1, the four stimulated electrode pairs were shifted in 0.75–mm steps over 3 mm in the apical-basal direction. In experiment 2, the four electrodes were closely spaced and positioned apically, medially, or basally. An additional condition spaced the four electrodes as widely as possible. In experiment 1, City University of New York sentence scores showed a significant decrease in performance as the electrodes were shifted basally; no other speech measures showed a significant change with electrode location. For experiment 2, all scores were the best with the processor that had the electrodes spaced as widely as possible. In both experiments, all 4–electrode SPEAK processors produced significantly poorer speech recognition than the subject's own 20–electrode processor. These results indicate that the location of electrodes is an important factor in implant performance.
Abbreviations: C = maximum comfortable loudness levels, CI = cochlear implant, CUNY = City University of New York, NU-6 = Northwestern University Auditory Test No. 6, Τ = electrical thresholds
Report of the Consensus Conference on the Diagnosis of Auditory Processing Disorders in School-Aged Children
Authors: Jerger James , Musiek Frank 
Published: April 2022

BACKGROUNDA group of 14 senior scientists and clinicians met at the Callier Center in Dallas over the 2–day period, April 27–28, 2000, in an attempt to reach a consensus on the problem of diagnosing auditory processing disorders in school-aged children. The conference was organized by James Jerger and Frank Musiek. The following individuals participated:Sharon Abel, PhD, University of Toronto, Toronto, ONJane Baran, PhD, University of Massachusetts, Amherst, MAAnthony Cacace, PhD, Albany Medical College, Albany, NYGail Chermak, PhD ‡, Washington State University, Pullman, WASusan Dalebout, PhD, University of Virginia, Charlottesville,VAJay Hall III, PhD, University of Florida, Gainesville, FLLinda Hood, PhD, Louisiana State University Medical Center, New Orleans, LALisa Hunter, PhD, University of Minnesota, Minneapolis, MNJames Jerger, PhD, University of Texas at Dallas, Dallas, TXSusan Jerger, PhD, University of Texas at Dallas, Dallas, TXRobert Keith, PhD, University of Cincinnati, Cincinnati, OHFrank Musiek, PhD, Dartmouth-Hitchcock Medical Center, Hanover, NHRoss Roeser, PhD, University of Texas at Dallas, Dallas, TXChristine Sloan, PhD, Annapolis Valley Regional School Board Berwick, NSMeeting both as separate groups and in plenary session, the conferees reached the consensus summarized below.