bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactive effects of molecular subtypes with tumor size and extracranial metastatic pattern on risk of brain metastasis in breast cancer patients: A population‐based study
Authors: Shen Bo , Li Jieqing , Yang Mei , Liu Kangkang , Zhang Junsheng , Li Weiping , Zhang Yi , Wang Kun 
Published: November 2022

AbstractBackgroundEarly detection of brain metastasis (BM) is essential for prognostic improvement in breast cancer (BC) patients. The aim was to identify predictors of BCBM in different molecular subtypes on a population‐based level.MethodsThe Surveillance, Epidemiology, and End Results database was used to select BC patients diagnosed from 2010 to 2018. We evaluated the incidence and risk factors of BCBM and tested the interaction effects between molecular subtypes and other risk factors.ResultsAmong the 527,525 selected patients, molecular subtypes significantly interacted with T stage and extracranial metastasis (ECM) patterns on the risk of BM in the whole BC population (interaction p = 0.002, <0.001, respectively) and after excluding patients with unknown states of key factors. BM development was independent of the T stage only in HR−/HER2− patients (trend p = 0.126). We selected BC patients with single‐organ ECM and found a significant interaction between molecular subtypes and ECM patterns (interaction p = 0.013). The impact of ECM patterns on the risk of BM was limited to HR−/HER2− patients (trend p < 0.001), for whom using bone metastasis as a reference, lung metastasis increased the risk of BM (OR = 1.936, 95% CI: 1.300–2.882, p = 0.001).ConclusionT stage and ECM patterns had different associations with BM in different molecular subtypes. HR−/HER2− BC had distinct features on BM development, manifested as a lack of tumor size effect and is associated with lung metastasis. Close surveillance for BM should be considered for HR−/HER2− BC patients.
“Hello, can you hear me?”: Narratives of online mental health counselling among Filipino adults during the pandemic
Authors: Dela Cruz Edlene Nicole M. , Marcelo Rohann Cris A. , Naling Bea Ysabelle M. , Ty Welison Evenston G. 
Published: November 2022

AbstractFilipinos are often reluctant to seek professional mental health (MH) help. However, literature reveals that online counselling is preferable to face‐to‐face (F2F) MH services, with the pandemic possibly magnifying technology's anonymity and disinhibiting benefits. To further explore facilitators of online MH help‐seeking, this study tapped into the lived stories of Filipino adults who experienced at least one counselling session from March 2020 to March 2022. Among 83 screened respondents, 11 semistructured interviews were transcribed and analysed following Crossley's (2000) method. Findings reveal an overarching online MH counselling narrative composed of three phases and their respective events: (a) Precounselling (Resiliency Narrative versus MH Awareness, Turning Point, and Linking versus Searching); (b) Counselling (Two‐way Introduction, Unloading and Untangling, and Decision to Maintain or Terminate); and (c) Postcounselling (Relapse and Journey towards Growth and Advocacy). Three all‐encompassing facilitators were embedded throughout the process: Autonomy, Financial Capability, and MH Institutions. Notably, participants attributed their MH help‐seeking to autonomy, despite culture and stigma, because of enabling environments that entail heightened MH literacy, positive past experiences with professional MH help, social support and encouragement, and a sense of shared reality. Benefits unique to online counselling were also apparent among participants' narratives, including alleviating geographical boundaries and added financial and personal pressures. Overall, findings posit that Filipino adults consider online counselling as a preferable alternative to F2F counselling, thus necessitating further development and institutional support.
Reversible Modulation of Aptamer‐Ligand Binding in RNA Light‐Up Aptamers Containing G‐Quadruplex Using Chemical Stimuli
Authors: Soni Rashi , Krishna A. Murali , More Shahaji H. , Sharma Ashwani 
Published: November 2022

AbstractRegulating a system in equilibrium transiently to out‐of‐equilibrium by using certain stimuli is the strategy used by natural biomolecules to function. Herein, we showed that the interaction of synthetic RNA aptamers, having a G‐quadruplex core structure, with their corresponding ligands could be regulated from their equilibrium state to non‐equilibrium state in a reversible manner using simple chemical stimuli (Ag+ and cysteine). The approach would be useful for designing aptamer regulators that work in a dynamic nucleic acid network, where a strict control on aptamer‐ligand interaction is needed. In addition, to the best of our knowledge, this is the first report which shows that RNA G‐quadruplexes can be disrupted by the addition of silver ions. This would be useful not only in designing RNA‐based sensors or regulators but would also be useful for understanding the role of metal ions in RNA folding and catalysis.
Bacterial Dehydrogenases Facilitate Oxidative Inactivation and Bioremediation of Chloramphenicol
Authors: Zhang Lei , Toplak Marina , Saleem‐Batcha Raspudin , Höing Lars , Jakob Roman , Jehmlich Nico , von Bergen Martin , Maier Timm , Teufel Robin 
Published: November 2022

AbstractAntimicrobial resistance represents a major threat to human health and knowledge of the underlying mechanisms is therefore vital. Here, we report the discovery and characterization of oxidoreductases that inactivate the broad‐spectrum antibiotic chloramphenicol via dual oxidation of the C3‐hydroxyl group. Accordingly, chloramphenicol oxidation either depends on standalone glucose‐methanol‐choline (GMC)‐type flavoenzymes, or on additional aldehyde dehydrogenases that boost overall turnover. These enzymes also enable the inactivation of the chloramphenicol analogues thiamphenicol and azidamfenicol, but not of the C3‐fluorinated florfenicol. Notably, distinct isofunctional enzymes can be found in Gram‐positive (e. g., Streptomyces sp.) and Gram‐negative (e. g., Sphingobium sp.) bacteria, which presumably evolved their selectivity for chloramphenicol independently based on phylogenetic analyses. Mechanistic and structural studies provide further insights into the catalytic mechanisms of these biotechnologically interesting enzymes, which, in sum, are both a curse and a blessing by contributing to the spread of antibiotic resistance as well as to the bioremediation of chloramphenicol.
Primary Ewing sarcoma of the liver: Diagnosis, management, and prognosis: A case report and literature review
Authors: Hedfi Mohamed , Ben Ismail Imen , Zenaidi Hakim , Bouslama Sirine , Zoghlami Ayoub 
Published: November 2022

AbstractEwing's sarcoma tumors (ES) are a rare entity exceptionally localized on the liver. We report a case of an ES of the liver in a 26‐year‐old man who presented with abdominal pain. The diagnosis was confirmed with a histopathological examination of the left hepatectomy specimen and adjuvant chemotherapy was received.
Siderophore‐Linked Ruthenium Catalysts for Targeted Allyl Ester Prodrug Activation within Bacterial Cells
Authors: Southwell James W. , Herman Reyme , Raines Daniel J. , Clarke Justin E. , Böswald Isabelle , Dreher Thorsten , Gutenthaler Sophie M. , Schubert Nicole , Seefeldt Jana , Metzler‐Nolte Nils , Thomas Gavin H. , Wilson Keith S. , Duhme‐Klair Anne‐Kathrin 
Published: November 2022

AbstractDue to rising resistance, new antibacterial strategies are needed, including methods for targeted antibiotic release. As targeting vectors, chelating molecules called siderophores that are released by bacteria to acquire iron have been investigated for conjugation to antibacterials, leading to the clinically approved drug cefiderocol. The use of small‐molecule catalysts for prodrug activation within cells has shown promise in recent years, and here we investigate siderophore‐linked ruthenium catalysts for the activation of antibacterial prodrugs within cells. Moxifloxacin‐based prodrugs were synthesised, and their catalyst‐mediated activation was demonstrated under anaerobic, biologically relevant conditions. In the absence of catalyst, decreased antibacterial activities were observed compared to moxifloxacin versus Escherichia coli K12 (BW25113). A series of siderophore‐linked ruthenium catalysts were investigated for prodrug activation, all of which displayed a combinative antibacterial effect with the prodrug, whereas a representative example displayed little toxicity against mammalian cell lines. By employing complementary bacterial growth assays, conjugates containing siderophore units based on catechol and azotochelin were found to be most promising for intracellular prodrug activation.
Dynamic global feature extraction and importance‐correlation selection for the prediction of concentrate copper grade and recovery rate
Authors: Wang Zhiqiang , Zhang Xintong , He Dakuo 
Published: November 2022

AbstractLarge‐scale industrial data have brought great challenges to data calculation and analysis. Feature extraction and selection have become one of the research emphases in data mining. To mine the dynamic characteristics of large‐scale industrial data, a dynamic global feature extraction (DGFE) method integrating principal component analysis (PCA) and kernel principal component analysis (KPCA) is proposed such that the achieved feature set is not only dynamic but also contains linear and non‐linear features. To ensure that the obtained feature set is optimal with the minimum redundancy, a new importance‐correlation‐based feature selection (ICFS) method is proposed. To verify the validity and feasibility of the proposed methods, the partial least square (PLS) and least square support vector machine (LSSVM) prediction models for the concentrate copper grade and the recovery rate are established. The effectiveness of the proposed methods is verified through data experiments on a copper flotation industrial process.
Insight into Key Parameters for Fabricating Stable Single‐Atom Pt‐Nix Alloy by Reduction Environment‐Induced Anti‐Ostwald Effects
Authors: Xiao Xin , Xi Shibo , Zang Wenjie , Lim San Hua , Gao Jiajian , Chu Wei , Liu Yan 
Published: November 2022

AbstractDeveloping single‐atom catalysts with superior stability under reduction conditions is essential for hydrogenation/dehydrogenation catalysis and green hydrogen generation. In this contribution, single‐atom Pt catalysts were achieved via a reduction environment‐induced anti‐Ostwald approach in the highly confined Ni species (Pt‐Nix) on nonreducible Al2O3 matrix. In‐situ X‐ray absorption spectroscopy indicated that the isolated Pt‐Nix metallic bonds, generated at high reduction temperature, dominated the formation of single Pt atoms. A relatively large cluster of metallic Ni would benefit the stabilization of Pt single atom as observed via high‐angle annular dark‐field scanning transmission electron microscopy and validated by density functional theory simulation. Excellent performance on cellulose hydrogenolysis was demonstrated under harsh reductive and hydrothermal conditions, potentially expandable to other hydrogen involved reactions like CO2 hydrogenation, green hydrogen production from different hydrogen carriers, and beyond.
Analysis of a coupled‐inductor double‐boost dc‐dc converter operating in discontinuous conduction mode
Authors: González Sergio Alberto , D'Amico María Belén 
Published: November 2022

AbstractIn this article, the steady‐state and dynamical behavior of a coupled‐inductor doble‐boost dc‐dc converter (CBC) operating in discontinuous conduction mode (DCM) is discussed. The CBC makes use of the magnetic coupling between the windings of two boost stages connected in cascade to achieve high voltage gains. Developments given here shown that the voltage stress on semiconductors can be reduced to values lower than the output voltage by selecting the relation between windings adequately. Moreover, the derivation of an averaged model predicts that the converter presents a first‐order dominant response at low frequencies. These properties add to other important CBC advantages such as low component count, high efficiency, and simple implementation and design. The operation in DCM is very attractive for applications where the primary source acts as a current generator, as in the case of photovoltaic panels or constant power loads. Based on the results, the CBC can be seen as an immediate substitute of the classical boost converter when high dc‐dc voltage gains are required. Different tests carried out on an experimental set‐up corroborate the analytical findings.
‘Deep understanding’ for anti‐racist school transformation: School leaders' professional development in the context of Black Lives Matter
Authors: Walker Sharon , Bennett Ian , Kettory Pavenjit , Pike Clare , Walker Lee 
Published: November 2022

AbstractIn June 2020, the world witnessed an upsurge in Black Lives Matter (BLM) demonstrations following the murder of George Floyd, an African American, by a White American police officer. The international response called for the global community to reassess the value of black lives blighted by racist social systems. The mass sentiment acted as a catalyst for educational institutions, including those in the UK, to mount a response. It is in this context that a School Partnership Group representing primary and secondary schools in East London embarked on developing a workshop series for the professional development of school leaders. The sessions were aimed at school transformation through anti‐racist educational approaches. In this article, we present a discussion of the workshop series held in the academic year 2020–2021, which brought school leaders together in a reflective community of practice. Drawing on data from focus group conversations carried out following the end of the series, this paper argues for school leaders' professional development that prioritises ‘deep understanding’ supported by reflective communities of practice as a pre‐requisite for effective anti‐racist practice and sustained school transformation.
Rapid detection and online analysis of microbial changes through flow cytometry
Authors: Kupschus Jonas , Janssen Stefan , Hoek Andreas , Kuska Jan , Rathjens Jonathan , Sonntag Carsten , Ickstadt Katja , Budzinski Lisa , Chang Hyun‐Dong , Rossi Andrea , Esser Charlotte , Hochrath Katrin 
Published: November 2022

AbstractShort‐read 16 S rRNA gene sequencing is the dominating technology to profile microbial communities in different habitats. Its uncontested taxonomic resolution paved the way for major contributions to the field. Sample measurement and analysis, that is, sequencing, is rather slow–in order of days. Alternatively, flow cytometry can be used to profile the microbiota of various sources within a few minutes per sample. To keep up with high measurement speed, we developed the open source‐analyzing tool FlowSoFine. To validate the ability to distinguish microbial profiles, we examined human skin samples of three body sites (N = 3 × 54) with flow cytometry and 16 S rRNA gene amplicon sequencing. Confirmed by sequencing of the very same samples, body site was found to be significantly different by flow cytometry. For a proof‐of‐principle multidimensional approach, using stool samples of patients (N = 40) with/without inflammatory bowel diseases, we could discriminate the health status by their bacterial patterns. In conclusion, FlowSoFine enables the generation and comparison of cytometric fingerprints of microbial communities from different sources. The implemented interface supports the user through all analytical steps to work out the biological relevant signals from raw measurements to publication ready figures. Furthermore, we present flow cytometry as a valid method for skin microbiota analysis.
The effects of iron injection on blood doping biomarkers in dried blood spots
Authors: Loria Francesco , Maret Estelle , Schobinger Céline , Kuuranne Tiia , Grabherr Silke , Leuenberger Nicolas 
Published: November 2022

AbstractIron supplementation is not considered as a doping method; however, it can affect the levels of several biomarkers of the hematologic module of the athlete biological passport (ABP), such as the reticulocyte percentage (%RET) and hemoglobin (HGB) level. Thus, iron injection could be a confounding factor in antidoping analyses. Previous studies have suggested that the HGB level and the expression levels of reticulocyte‐related‐mRNAs, such as 5′‐aminolevulinate synthase 2 (ALAS2) and carbonic anhydrase 1 (CA1), could be promising biomarkers for the ABP and detectable in dried blood spots (DBSs). Therefore, in this study, we examined the impact of iron injection on the levels of these potential biomarkers in DBSs. Reticulocyte‐related‐mRNAs analyses were performed by RT‐qPCR. Ferritin level in DBS was measured with enzyme‐linked immunosorbent assay (ELISA) method. Notably, there were no significant effects of iron supplementation on the levels of ALAS2 and CA1 mRNAs but by contrast, the %RET and immature reticulocyte fraction (IRF) measured in whole blood increased significantly following iron injection. As expected, iron supplementation increased the ferritin level significantly in both serum and DBS samples. In conclusion, these findings reinforce the specificity of reticulocyte‐related mRNAs in DBSs as biomarkers of blood doping to target in antidoping analyses.
A comparative analysis of the ontogeny of syngnathids (pipefishes and seahorses) reveals how heterochrony contributed to their diversification
Authors: Schneider Ralf F. , Woltering Joost M. , Adriaens Dominique , Roth Olivia 
Published: November 2022

AbstractBackgroundSyngnathids are a highly derived and diverse fish clade comprising the pipefishes, pipe‐horses, and seahorses. They are characterized by a plethora of iconic traits that increasingly capture the attention of biologists, including geneticists, ecologists, and developmental biologists. The current understanding of the origins of their derived body plan is, however, hampered by incomplete and limited descriptions of the early syngnathid ontogeny.ResultsWe provide a comprehensive description of the development of Nerophis ophidion, Syngnathus typhle, and Hippocampus erectus from early cleavage stages to release from the male brooding organ and beyond, including juvenile development. We comparatively describe skeletogenesis with a particular focus on dermal bony plates, the snout‐like jaw morphology, and appendages.ConclusionsThis most comprehensive and detailed account of syngnathid development to date suggests that convergent phenotypes (e.g., reduction and loss of the caudal fins), likely arose by distinct ontogenetic means in pipefishes and seahorses. Comparison of the ontogenetic trajectories of S. typhle and H. erectus provides indications that characteristic features of the seahorse body plan result from developmental truncation. Altogether, this work provides a valuable resource and framework for future research to understand the evolution of the outlandish syngnathid morphology from a developmental perspective.
The effective doses of remimazolam besylate in the procedural sedation of endoscopic retrograde cholangiopancreatography
Authors: Tan Dan‐Dan , Gu Jin , Li Juan , Yu Wan‐Qiu , Liu De‐Xing , Zhao Li‐Jin , Zhu Guo‐Hua , Yang Xin‐Xin , Tian Jin , Tian Qi , Zhu Zhao‐Qiong 
Published: November 2022

AbstractThis study aimed to determine the values of the half‐effective dose (ED50) and 95% effective dose (ED95) of remimazolam besylate used in the procedural sedation of endoscopic retrograde cholangiopancreatography (ERCP). Sixty patients who fulfilled the inclusion and exclusion criteria of this study were selected. Sufentanil was administered intravenously and remimazolam besylate was administered 2 min later. ERCP treatment was feasible when the modified alertness/sedation (MOAA/S) score was ≤2. If choking or movement occurred during duodenoscope placement, it was considered as a positive reaction. The dose was increased in the next patient; otherwise, it was considered as a negative reaction, and the dose was reduced in the next patient. The ED50 and ED95 values and 95% confidence interval (CI) of remimazolam besylate were calculated by Probit regression analysis. All 60 patients completed the trial. The ED50 and ED95 values of remimazolam besylate were 0.196 and 0.239 mg/kg, respectively, for the procedural sedation of ERCP. The time of MOAA/S score ≤ 2 was (82.58 ± 21.70) s, and the mean time of awakening was (9.03 ± 5.64) min. Transient hypotension was observed in two patients without medical intervention. The ED50 and ED95 values of remimazolam besylate used in the procedural sedation of ERCP were 0.196 and 0.239 mg/kg, and the dose of the medications has definite efficacy and good safety.
A typically progressive dissection of the internal carotid artery with recurrent hiccups: A case report with continuous 2‐year data recording
Authors: Zhang Zong‐Min , Yang Xin‐Xin , Ling Li , Zhou Man‐Hong 
Published: November 2022

AbstractPatients with internal carotid artery dissection (ICAD) usually report headache, neck pain, Horner's syndrome, and ischemic stroke. Because the posterior cranial nerve is involved, some patients may show different forms of posterior cranial nerve paralysis. There have been no reports of patients with ICAD showing repeated hiccups. Here, to help clinicians identify ICAD early and gain a better understanding of the atypical manifestations of the disease, we report an atypical case of recurrent hiccup symptoms caused by ICAD.
Risk factors for bladder injury during placenta accreta spectrum surgery
Authors: Friedrich Lior , Mor Nizan , Weissmann‐Brenner Alina , Kassif Eran , Friedrich Shakad Noah , Weissbach Tal , Castel Elias , Levin Gabriel , Meyer Raanan 
Published: November 2022

AbstractObjectivesTo identify risk factors associated with bladder injury during placenta accreta spectrum (PAS) surgeries.MethodsThis retrospective cohort study was conducted at the Chaim Sheba Medical Center. The study population included pregnant women diagnosed with PAS undergoing uterine‐preserving surgery or hysterectomy. Women with and without operative bladder injury were compared by univariate analysis followed by multivariate analysis. A sub‐analysis of women without preoperative sonographic suspicion of bladder invasion was performed.ResultsA total of 312 women were included in the study. Bladder injury incidence was 9.3% (n = 29). Uterine preservation was performed in 267/312 (85.6%) women. The number of previous cesarean deliveries and a preoperative sonogram suspicious for placenta percreta were found to be independent risk factors for intraoperative bladder injury (odds ratio [OR] 1.30, P = 0.019, and OR 5.23, P = 0.002, respectively). The number of previous cesarean deliveries and preoperative sonographic suspicion of placenta percreta were also associated with bladder injury in the sub‐analysis (OR 1.30, P = 0.044 for previous cesarean deliveries, and OR 3.36, P = 0.036, for preoperative suspicion of bladder injury).ConclusionThe number of previous cesarean deliveries and preoperative suspicion of placenta percreta are preoperative factors that can assist in preoperative planning and intraoperative management of PAS cases.