bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential role of MALT1 as a candidate biomarker of disease surveillance and treatment response prediction in inflammatory bowel disease patients
Authors: Wu Zhigang , Bi Yingyan 
Published: January 2022

AbstractBackgroundMucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1) regulates adaptive and innate immune responses in several inflammatory disease. However, clinical involvement of MALT1 in inflammatory bowel disease (IBD) patients remains unclear. Hence, this study was intended to investigate the correlation of blood MALT1 with disease activity, inflammation indexes as well as treatment response of IBD patients.MethodsBlood MALT1 expression in 100 IBD patients [including 25 active (A)‐Crohn's disease (CD) patients, 25 remission (R)‐CD patients, 25 A‐ulcerative colitis (UC) patients, and 25 R‐UC patients] and 25 health controls (HCs) was detected by reverse transcription‐quantitative polymerase chain reaction; besides, serum tumor necrosis factor‐alpha (TNF‐α) and interleukin‐17A (IL‐17A) in IBD patients were detected by enzyme‐linked immunosorbent assay.ResultsMALT1 was increased in A‐UC patients than in R‐UC patients (p = 0.038) and in HCs (p < 0.001), and also elevated in A‐CD patients than in R‐CD patients (p = 0.048) and in HCs (p < 0.001). MALT1 was positively related to C‐reactive protein (CRP, p = 0.011), TNF‐α (p = 0.036), IL‐17A (p = 0.023), and Mayo score (p = 0.005) in A‐UC patients, CRP (p = 0.017), erythrocyte sedimentation rate (p = 0.033), TNF‐α (p = 0.004), and Crohn's disease activity index score (p = 0.028) in A‐CD patients. But MALT1 was not correlated with either inflammation indexes or disease activity score in R‐UC and R‐CD patients. MALT1 gradually declined from baseline to W12 in A‐UC and A‐CD patients (both p < 0.001). Moreover, MALT1 at W4 (p = 0.031) and W12 (p = 0.003) in A‐UC patients as well as MALT1 at W12 (p = 0.008) in A‐CD patients associated with clinical response.ConclusionMALT1 serves as a potential biomarker for disease surveillance and treatment response prediction of IBD patients.
Relation of circulating lncRNA GAS5 and miR‐21 with biochemical indexes, stenosis severity, and inflammatory cytokines in coronary heart disease patients
Authors: Jiang Yan , Du Tian 
Published: January 2022

AbstractBackgroundLong noncoding RNA GAS5 (lnc‐GAS5) and its target microRNA‐21 (miR‐21) regulate blood lipid, macrophages, Th cells, vascular smooth muscle cells to participate in atherosclerosis, and related coronary heart disease (CHD). The study aimed to further explore the linkage of their circulating expressions with common biochemical indexes, stenosis severity and inflammatory cytokines in CHD patients.MethodsNinety‐eight CHD patients and 100 controls confirmed by coronary angiography were enrolled. Plasma samples were collected for lnc‐GAS5 and miR‐21 detection by reverse transcription‐quantitative polymerase chain reaction and inflammatory cytokines determination by enzyme‐linked immunosorbent assay.ResultsLnc‐GAS5 was increased in CHD patients compared with controls (2.270 (interquartile range [IQR]: 1.676–3.389) vs. 0.999 ([IQR: 0.602–1.409], p < 0.001), whereas miR‐21 showed opposite tread (0.442 [IQR: 0.318–0.698] vs. 0.997 [IQR: 0.774–1.368], p < 0.001). In aspect of their intercorrelation, lnc‐GAS5 negatively linked with miR‐21 in CHD patients (p < 0.001) instead of controls (p = 0.211). Interestingly, among the common biochemical indexes, lnc‐GAS5 related to decreased high‐density lipoprotein cholesterol (p = 0.008) and increased C‐reactive protein (CRP) (p < 0.001), while miR‐21 correlated with lower total cholesterol (p = 0.024) and CRP (p < 0.001) in CHD patients. As stenosis degree, lnc‐GAS5 positively correlated with Gensini score (p < 0.001), but miR‐21 exhibited negative association (p = 0.003) in CHD patients. In terms of inflammatory cytokines, lnc‐GAS5 positively related to tumor necrosis factor α (TNF‐α) and interleukin (IL)‐17A, while miR‐21 negatively linked with TNF‐α, IL‐1β, IL‐6, and IL‐17 in CHD patients (all p < 0.05).ConclusionCirculating lnc‐GAS5 and its target miR‐21 exhibit potency to serve as biomarkers for CHD management.
The evaluation of the overexpression of theERG‐11,MDR‐1,CDR‐1, andCDR‐2genes in fluconazole‐resistantCandida albicansisolated from Ahvazian cancer patients with oral candidiasis
Authors: Maheronnaghsh Mehrnoush , Teimoori Ali , Dehghan Parvin , Fatahinia Mahnaz 
Published: January 2022

AbstractIntroductionResistance to azole drugs has been observed in candidiasis due to their long‐term use and poor response to treatment. Resistance to azole drugs inCandida albicansisolates is controlled by several genes includingERG11,CDR1,CDR2, andMDR1. In this study, the expression of the mentioned genes was evaluated inC. albicansisolates susceptible and resistant to fluconazole.MethodsAfter identifying theCandida isolatesusing morphological and molecular methods, the minimum inhibitory concentration (MIC) and drug susceptibility were determined using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) method. RNA was then extracted and cDNA was synthesized from 24C. albicansisolates from patients with cancer. Then, the mean expressions of these genes were compared in two groups using real‐time polymerase chain reaction (RT‐PCR).ResultsA total of 74Candidaisolates were obtained from the oral cavity of 61 cancer patients with oral candidiasis. After 24 h, 21.6% of the isolates were fluconazole‐resistant, 10.8% were identified as dose‐dependent, and the rest of the isolates (67.6%) were fluconazole‐sensitive. The mean expressions of theCDR1andMDR1 genes were significantly higher in the resistant isolates than in the sensitive ones. However, theERG11andCDR2 genes were not significantly increased in the resistant isolates.ConclusionThe increased mean expressions of theCDR1andMDR1 genes had a greater effect on fluconazole resistance among the drug‐resistant strains ofC. albicansin chemotherapy patients. It seemed that the accumulation of chemotherapeutic drugs in this organism stimulated some regulatory factors and increased the expression of these two genes and ultimately helped to further increase their expression and resistance to fluconazole.
One‐step and sequential SARSCOV‐2 polymerase chain reaction tests would not work every time
Authors: Hatami Firouze , Rabiei Mohammad Mahdi , Javandoust Gharehbagh Farid , Pourhoseingholi Mohamad Amin , Sabeti Shahram , Kheyrian Mahnaz , Alavi Darazam Ilad 
Published: January 2022

AbstractIntroductionRT‐PCR is widely used as a diagnostic test for the detection of SARS‐CoV‐2. In this study, we aim to describe the clinical utility of serial PCR testing in the final detection of COVID‐19.MethodWe collected multiple nasopharyngeal swab samples from patients who had negative RT‐PCR test on the first day after hospitalization. RT‐PCR tests were performed on the second day for all patients with initial negative result. For the patients with secondary negative results on day 2, tertiary RT‐PCR tests were performed on day 3 after hospitalization.ResultAmong 68 patients with initial negative test results, at the end of follow‐up, the mortality number was 20 (29.4%). About 33.8% of patients had subsequent positive PCR test results for the second time and 17.4% of the patients who performed third PCR test had positive result.ConclusionBased on this study, serial RT‐PCR testing is unlikely to yield additional information.
Theorizing the notion of well‐being in Islam: An update of Ryff's theory of eudaimonic psychological well‐being
Authors: Koburtay Tamer , Syed Jawad , Salhi Nidal Al 
Published: January 2022

AbstractPrior studies suggest that knowledge about workers' psychological well‐being is overly focused on materialism or biologically‐based understanding, not taking into account the role of spirituality in one's well‐being. Drawing on Ryff's (1989) psychological well‐being framework and using an interdisciplinary approach, this paper offers a model of 'well‐being in Islam' through theorizing this concept from an Islamic lens and contextually studying the implications of Islamic practices and spiritual facilities for employees' psychological well‐being in workplaces. Drawing on qualitative data collected from 22 employees, our model (findings) shows that worshipping Allah, contemplative practices, and patience are key elements of one's well‐being. This paper also points towards the important role of specific spiritual provisions (e.g., designated rooms for prayer, prayer time and ablution facilities) and contemplative practices (e.g., ritualistic‐cyclic, creative process, generative, movement practices, stillness, activist, relational) for Ryff's six dimensions of employees' eudaimonic well‐being. This study is unique as it integrates the notion of well‐being in Islam into management and organization studies and offers a novel and contextual extension of Ryff's theory by integrating a spiritual notion of well‐being. In the end, theoretical and practical implications are offered.
Does the Internal Carotid Artery Attenuate Blood‐Flow Pulsatility in Small Vessel Disease? A 7 T 4D‐Flow MRI Study
Authors: van Tuijl Rick J. , Ruigrok Ynte M. , Geurts Lennart J. , van der Schaaf Irene C. , Biessels Geert Jan , Rinkel Gabriël J. E. , Velthuis Birgitta K. , Zwanenburg Jaco J. M. 
Published: January 2022

BackgroundIncreased cerebral blood‐flow pulsatility is associated with cerebral small vessel disease (cSVD). Reduced pulsatility attenuation over the internal carotid artery (ICA) could be a contributing factor to the development of cSVD and could be associated with intracranial ICA calcification (iICAC).PurposeTo compare pulsatility, pulsatility attenuation, and distensibility along the ICA between patients with cSVD and controls and to assess the association between iICAC and pulsatility and distensibility.Study TypeRetrospective, explorative cross‐sectional study.SubjectsA total of 17 patients with cSVD, manifested as lacunar infarcts or deep intracerebral hemorrhage, and 17 age‐ and sex‐matched controls.Field Strength/SequenceThree‐dimensional (3D) T1‐weighted gradient echo imaging and 4D phase‐contrast (PC) MRI with a 3D time‐resolved velocity encoded gradient echo sequence at 7 T.AssessmentBlood‐flow velocity pulsatility index (vPI) and arterial distensibility were calculated for seven ICA segments (C1–C7). iICAC presence and volume were determined from available brain CT scans (acquired as part of standard clinical care) in patients with cSVD.Statistical TestsIndependent t‐tests and linear mixed models. The threshold for statistically significance was P < 0.05 (two tailed).ResultsThe cSVD group showed significantly higher ICA vPI and significantly lower distensibility compared to controls. Controls showed significant attenuation of vPI over the carotid siphon (−4.9% ± 3.6%). In contrast, patients with cSVD showed no attenuation, but a significant increase of vPI (+6.5% ± 3.1%). iICAC presence and volume correlated positively with vPI (r = 0.578) in patients with cSVD and negatively with distensibility (r = −0.386).ConclusionDecreased distensibility and reduced pulsatility attenuation are associated with increased iICAC and may contribute to cSVD. Confirmation in a larger prospective study is required.Evidence Level2Technical EfficacyStage 2
LncRNA LINC00924 upregulates NDRG2 to inhibit epithelial‐mesenchymal transition via sponging miR‐6755‐5p in hepatitis B virus‐related hepatocellular carcinoma
Authors: Yu Kai , Mei Yunhua , Wang Zhongyi , Liu Bo , Deng Ming 
Published: January 2022

AbstractHepatitis B virus (HBV)‐related hepatocellular carcinoma (HCC) is a life‐threatening cancer. Long noncoding RNAs participate in HBV‐related HCC progression. Based on the bioinformatics analysis, LINC00924 downregulation is positively related to unfavorable outcomes in patients with HBV‐related HCC. Herein, we detected the biological functions and regulatory system of LINC00924 in HCC. The LINC00924 downregulation in HBV‐related HCC tissues and cells was revealed by reverse transcription‐quantitative polymerase chain reaction. Functionally, as Transwell assays and western blotting indicated, LINC00924 elevation inhibited HCC cell invasion and epithelial‐mesenchymal transition (EMT). The binding site between LINC00924 and miR‐6755‐5p was determined by luciferase reporter assays. miR‐6755‐5p was confirmed to target NDRG2. miR‐6755‐5p upregulation decreased NDRG2 messenger RNA (mRNA) and protein levels. The mRNA and protein levels of NDRG2 were downregulated in tissues and cells. NDRG2 knockdown attenuated the inhibition induced by LINC00924 overexpression on invasion and EMT of HCC cells. In summary, LINC00924 increases NDRG2 expression to inhibit EMT by targeting miR‐6755‐5p in HBV‐related HCC.
A novel risk score model based on glycolysis‐related genes and a prognostic model for predicting overall survival of osteosarcoma patients
Authors: Chen Wenhao , Lin Yuxiang , Huang Jianping , Yan Zhiyu , Cao Hua 
Published: January 2022

AbstractThis study aims to construct a novel risk score model based on glycolysis‐related genes in osteosarcoma and to build and validate a prognostic model for predicting overall survival of patients with osteosarcoma. The transcriptome data and corresponding clinical data of patients with osteosarcoma were obtained from The Cancer Genome Atlas (TCGA) as the training set, and from Gene Expression Omnibus (GEO) database as the validation set. Univariate Cox regression analysis was used to screen the prognostic glycolysis‐related genes. The risk coefficient of each glycolysis‐related gene was calculated using LASSO regression analysis. Using the median risk score as the cut‐off point, patients were divided into high‐risk and low‐risk groups. Kaplan–Meier survival analysis was used to determine whether there was a significant difference in the overall survival between the two groups. The nomogram was constructed according to the results of multivariate Cox regression. The C‐index was calculated, the calibration chart, clinical decision curve and receiver operating characteristic curve were drawn to evaluate the predictive performance of the nomogram. We performed Gene Ontology and Kyoto encyclopedia of genes and genomics enrichment analysis to explore the potential mechanism of prognostic‐related glycolysis genes in osteosarcoma. A total of 88 and 53 cases were obtained from the TCGA and GEO database, respectively. A total of 10 key glycolytic genes related to prognosis were screened out. The Kaplan–Meier survival curve revealed that the overall survival of the high‐risk group was significantly shorter than that of the low‐risk group. The C indices of the training set and the verification set were 0.882 and 0.828, respectively. Our findings will provide further understanding of clinical prognostic outcomes of osteosarcoma patients.
Slovak raw goat milk as a source of variable, biofilm‐forming staphylococci, and their susceptibility to lantibiotic bacteriocins
Authors: Lauková Andrea , Pogány Simonová Monika , Focková Valentína , Tomáška Martin , Drončovský Maroš , Vargová Miroslava , Dvorožňáková Emília 
Published: January 2022

AbstractBackgroundGoat milk and products from it are popular with the public in Slovakia. Consumers demand safe food. Therefore, methods to eliminate spoilage bacteria are necessary. This study focuses on susceptibility to lantibiotic bacteriocins of variable staphylococci, which were isolated from raw goat milk. A total of 53 raw goat milk samples collected from 283 goats in the regions of central and eastern Slovakia were analyzed.ResultsIn total, 37 strains were identified to use a combination of matrix‐assisted laser desorption ionization time‐of‐flight spectrometry and phenotypization. They were allotted to 14 species, which can be involved in 7 different clusters. Staphylococci showed mostly low‐grade biofilm formation ability; six different strains were high‐grade biofilm‐forming. Staphylococcus xylosus SX 17/1 showed the highest biofilm formation ability value (3.16 ± 1.77). Staphylococci were deoxyribonuclease and mostly hemolysis negative (γ‐hemolysis). They all were susceptible to bacteriocins (inhibition activity up to 204,800 AU/ml).ConclusionsVariable staphylococcal species were detected in raw goat milk, mostly coagulase‐negative species, involving Staphylococcus arlettae. The species Staphylococcus hominis dominated. However, also the coagulase‐positive species Staphylococcus delphini and Staphylococcus schleiferi were detected. This is the first study involving detailed testing of staphylococci from Slovak raw goat milk and indication of a promising practical tool and prophylaxis using bacteriocins.
Encapsulation of Zanthoxylum bungeanum essential oil to enhance flavor stability and inhibit lipid oxidation of Chinese‐style sausage
Authors: Meng Fan‐Bing , Lei Yu‐Ting , Zhang Qian , Li Yun‐Cheng , Chen Wei‐Jun , Liu Da‐Yu 
Published: January 2022

AbstractBACKGROUNDZanthoxylum bungeanum essential oil (ZBEO) is a popular seasoning, commonly used in the food industry. It contains many easily degraded and highly volatile bioactive substances. Control of the stability of the bioactive substances in ZBEO is therefore very important in the food industry.RESULTSIn this study, microencapsulation was applied to improve ZBEO stability. The key parameters for microcapsule preparation were optimized by the Box–Behnken design method, and the optimum conditions were as follows: ratio of core to wall, 1:8; ratio of hydroxypropyl‐α‐cyclodextrin (HPCD) to soy protein isolate (SPI), 4; total solids content, 12%; and homogenization speed, 12 000 rpm. Antioxidant experiments have indicated that tea polyphenols (TPPs) effectively inhibited hydroxy‐α‐sanshool degradation in ZBEO microcapsules. Application of ZBEO microcapsules in Chinese‐style sausage effectively inhibited lipid oxidation in sausages and protected hydroxy‐α‐sanshool and typical volatiles from volatilization and degradation during sausage storage.CONCLUSIONThe results suggested that ZBEO microencapsulation is an effective strategy for improving the stability of its bioactive components and flavor ingredients during food processing. © 2022 Society of Chemical Industry.
Molecular mechanism of high‐production tannase of Aspergillus carbonariusNCUF M8 after ARTP mutagenesis: revealed by RNA‐seq and molecular docking
Authors: Dong Xianxian , Wan Yin , Chen Yanru , Wu Xiaojiang , Zhang Yulong , Deng Mengfei , Cai Wenqin , Wu Xiaodan , Fu Guiming 
Published: January 2022

AbstractBACKGROUNDTannase is an enzyme produced by microbial fermentation and is widely used in the food industry; however, the molecular mechanism of tannase production by Aspergillus has not yet been studied. This study was conducted to reveal the differences in Aspergillus carbonarius tannase enzymatic characterization, secondary structures and molecular mechanisms after treatment of the strain with atmospheric and room temperature plasma (ARTP).RESULTSThe results showed that the specific activity of tannase was improved by ARTP treatment, and it showed higher thermostability and tolerance to metal ions and additives. The enzymatic characterization and molecular docking results indicated that tannase had a higher affinity and catalytic rate with tannic acid as a substrate after ARTP treatment. In addition, the docking results indicated that Aspergillus tannases may catalyze tannic acid by forming two hydrogen‐bonding networks with neighboring residues. RNA‐seq analysis indicated that changes in steroid biosynthesis, glutathione metabolism, glycerolipid metabolism, oxidative phosphorylation pathway and mitogen‐activated protein kinase signaling pathways might be crucial reasons for the high production of tannase.CONCLUSIONARTP enhanced the yield and properties of A. carbonarius tannase by changing the enzyme structure and cell metabolism. This study provides a theoretical basis for elucidating the molecular mechanism underlying high production of Aspergillus tannases. © 2022 Society of Chemical Industry.
Detection of soybean oil adulteration in cow ghee (clarified milk fat): an ultrafast study using flash gas chromatography electronic nose coupled with multivariate chemometrics
Authors: Roy Mrinmoy , Doddappa Manoj , Yadav Binod K , Jaganmohan Rangarajan , Sinija Vadakkepulppara RN , Manickam Loganathan , Sarvanan Shanmugasundaram 
Published: January 2022

AbstractBACKGROUNDCow ghee is one of the expensive edible fats in the dairy sector. Ghee is often adulterated with low‐priced edible oils, like soybean oil, owing to its high market demand. The existing adulteration detection methods are time‐consuming, requiring sample preparation and expertise in these fields. The possibility of detecting soybean oil adulteration (from 10% to 100%) in pure cow ghee was investigated in this study. The fingerprint information of volatile compounds was collected using a flash gas chromatography electronic nose (FGCEN) instrument. The classification results were studied using the pattern recognition chemometric models principal component analysis (PCA), soft independent modelling of class analogy (SIMCA), and discriminant function analysis (DFA).RESULTSThe most powerful fingerprint odor of all the samples identified from FGCEN analysis was acetaldehyde (Z)‐4‐heptenal, 2‐propanol, ethyl propanoate, and pentan‐2‐one. The odor analysis investigation was accomplished with an average analysis time of 90 s. A clear differentiation of all the samples with an excellent classification accuracy of more than 99% was achieved with the PCA and DFA chemometric methods. However, the results of the SIMCA model showed that SIMCA could only be used to detect ghee adulteration at higher concentration levels (30% to 100%). The validation study shows good agreement between FGCEN and gas chromatography–mass spectrometry methods.CONCLUSIONThe methodology demonstrated coupled with PCA and DFA methods for adulteration detection in ghee using FGCEN apparatus has been an efficient and convenient technique. This study explored the capability of the FGCEN instrument to tackle the adulteration problems in ghee. © 2022 Society of Chemical Industry.