bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Relation of Individual and Collective Narcissism and Belief in COVID-19 Conspiracy Theories: The Moderating Effects of Needs for Uniqueness and Belonging
Authors: Ük Bengi , Bahçekapılı Hasan Galip 
Published: May 2022

Abstract
The international pandemic of Covid-19 not only led to chaos and uncertainty, but it also brought about many conspiracy theories. In the last two decades, with a considerable amount of research, social psychologists have begun to understand the psychological factors behind conspiracy theories. One such factor is narcissism. In this research, we focus on both collective and grandiose narcissism’s predictive effects on Covid-19 conspiracy theories and possible moderation of need for uniqueness and belonging. In a Turkish sample (N= 309), we found that both collective and grandiose narcissism were significant predictors of Covid-19 conspiracy. In addition, when the need to feel special was high, grandiose narcissists, but not collective narcissists, tended to believe in Covid-19 conspiracies. Finally, we found that generic conspiracy beliefs and religiosity were also important predictors of Covid-19 conspiracy theories. Our research illuminates the link between narcissism and Covid-19 conspiracy theories. Future research should look for other possible moderating factors between collective narcissism and conspiracy beliefs.
LncRNA HOXA11-AS promotes gliomas malignant phenotypes and reduces its sensitivity to ROS via Tpl2-MEK1/2-ERK1/2 pathway
Authors: Wei Cheng , Zhang Xiaoyang , Peng Dazhao , Lu Yalin , Luo Lin , Wang Bo , Li Zesheng , He Yingjie , Du Xuezhi , Zhang Shu , Liang Hao , Li Shenghui , Wang Sheng , Han Lei , Zhang Jianning 
Published: May 2022

Abstract
Background
Dysregulation of the long noncoding RNA (lncRNA) HOXA11-AS plays an important role in the development of gliomas. Hence, uncovering the molecular mechanisms underlying abnormal expression of lncRNAs is important for identifying novel therapeutic targets in glioma.
Methods
The expression of HOXA11-AS, and the relationship between HOXA11-AS and the prognosis of patients with glioma were analyzed using databases and glioma samples. CCK-8, EdU, wound healing, transwell, and subcutaneous xenograft assays were performed to study the effects of HOXA11-AS on the proliferation, migration, and invasion of glioma cells in vitro and in vivo. Transcriptomics, small RNA sequencing, RIP, ChIRP, luciferase, and ChIP assays were used to identify the relationship between HOXA11-AS and let-7b-5p, and to explore its downstream targets in glioma. The downstream targets of HOXA11-AS were explored using transcriptomic, proteomic, and bioinformatic analyses. The role of HOXA11-AS in reactive oxygen species (ROS) sensitivity in gliomas was determined by ROS sensitivity experiments and xenograft orthotopic model assays in vitro and in vivo.
Results
HOXA11-AS was significantly upregulated in gliomas, and was negatively correlated with the prognosis of patients with glioma. Ectopic expression of HOXA11-AS promoted the proliferation, migration, and invasion of glioma cells in vitro and in vivo. Mechanistically, HOXA11-AS competitively acts as a miRNA sponge by binding to let-7b-5p, to increase the expression of CTHRC1, which activates the β-catenin/c-Myc pathway. HOXA11-AS recruits c-Jun to the Tpl2 promoter to induce Tpl2 transcription, which activates the Tpl2-MEK1/2-ERK1/2 pathway to promote ROS resistance. HOXA11-AS knockdown increased ROS sensitivity of glioma cells.
Conclusions
HOXA11-AS upregulates CTHRC1 expression by binding to let-7b-5p, which promotes β-catenin nuclear accumulation and activates c-Myc to promote the transcription of HOXA11-AS itself. HOXA11-AS knockdown promotes ROS sensitivity in glioma cells by regulating the Tpl2-MEK1/2-ERK1/2 axis. Our results highlight the potential of HOXA11-AS as a biomarker and therapeutic target in gliomas.
Annexin A1 peptide Ac2-26 mitigates ventilator-induced lung injury in acute respiratory distress syndrome rats by enhancing endothelial nitric oxide synthase activity
Authors: Ju Yingnan , Sun Xikun , Xu Guangxiao , Tai Qihang , Gao Wei 
Published: May 2022

Abstract
Background
Although mechanical ventilation is an essential support for acute respiratory distress syndrome (ARDS), ventilation also leads to ventilator-induced lung injury (VILI). This study aimed to estimate the effect and mechanism of Annexin A1 peptide (Ac2-26) on VILI in ARDS rats.
Methods
Thirty-two rats were randomized into the sham (S), mechanical ventilation (V), mechanical ventilation/Ac2-26 (VA) and mechanical ventilation/Ac2-26/L-NIO (VAL) groups. The S group only received anesthesia, and the other 3 groups received endotoxin and then ventilation for 4 h. Rats in the V, VA and VAL groups received saline, Ac2-26 and A c2-26/L-NIO, respectively. The gas exchange and capillary permeability were detected. The pulmonary and systemic inflammation were investigated. The oxidative stress response was also detected. Histological injury and apoptosis in lung tissues were tested. Moreover, the epithelial cells were also cultured to detect the effect of Ac2-26 on apoptosis induced by ARDS.
Results
All indexes deteriorated in the V, VA and VAL groups compared with the S group. Compared with V group, the PaO2/FiO2 ratio was increased, but the wet-to-dry weight ratio and protein levels in BALF were decreased in the VA group. The inflammatory cells and proinflammatory factors were reduced by Ac2-26. The oxidative stress response, lung injury and apoptosis were also decreased by Ac2-26 compared to V group. All improvements of Ac2-26 were partly reversed by L-NIO.
Conclusions
Ac2-26 effectively reduced VILI, improved respiratory function and alveolar-capillary permeability, ameliorated endothelial injury, inhibited oxygen stress and inflammation, and reduced lung injury and apoptosis. Ac2-26-mediated protection of VILI in ARDS rats partly depended on the endothelial nitric oxide synthase pathway.
Evaluation of candidate reference genes for quantitative real-time PCR normalization in red deer growing antler
Authors: Broggini Camilla , Abril Nieves , Carranza Juan , Membrillo Alberto 
Published: May 2022

Abstract
Sexual selection favors male traits that increase their ability to monopolize the breeding access to several females. Deer antlers are cranial appendages that regenerate annually in males. Throughout life, the phenology of antler growth advances and antler mass increases until the buck reaches, between 8 and 10 years old, maximum body mass and highest reproductive success. The molecular mechanisms of antler growth are of great interest in both evolutionary and regenerative medicine studies. To minimize errors in the assessment of gene expression levels by qRT-PCR, we analyzed the stability of a panel of eight candidate reference genes and concluded that qRT-PCR normalization to three stable genes is strongly convenient in experiments performed in red deer antler blood. To validate our proposal, we compared the expression level of three genes linked to red deer antler growth (ANXA2, APOD and TPM1) in fifteen male red deer classified as young (up to 4 years old) and adults (4–6 years old). Our data confirms that B2M, ACTB and RPLP0 are valuable reference genes for future gene expression studies in red deer antler blood, which would provide increased insight into the effects of intrinsic factors that determine the reproductive success of male red deer.
Serious hazards of transfusion: Evaluating the dangers of a wrong patient autologous salvaged blood in cardiac surgery
Authors: Uramatsu Masashi , Maeda Hideyuki , Mishima Shiro , Takahashi Megumi , Wada Jun , Amano Kagehiro , Barach Paul , Miki Tamotsu 
Published: May 2022

Abstract
Background: Intraoperative cell salvage is widely used, but the risk of incorrect administration remains underappreciated. We report on a case in which blood collected from a patient with lung cancer was mistakenly administered to a patient undergoing cardiac surgery who should have received his own collected blood. The initial investigation found that violations of procedures by field personnel were the cause. However, a detailed investigation revealed that not only violations were the cause, but also that the underlying causes included organizational policies, communication, workload and staffing deficiencies, human factors and cultural challenges. Case presentation: A 72-year-old male patient requiring dialysis due to chronic renal failure was admitted to our hospital for angina pectoris and multi-vessel coronary artery disease; cardiac surgery was performed using the autologous salvaged blood system with autologous blood collection, with no major problems other than prolonged operative time. A drop in blood pressure that night led to administering the patient's previously stored blood. The nurse took a blood bag from the ICU refrigerator and administered it to the patient. In doing so, neither the physician nor the nurse performed the requisite verification procedures. However, the blood administered was blood from another patient that had been collected the day before from another surgery. The ABO-mismatch transfusion had occurred and the patient was diagnosed with DIC. The patient recovered and was discharged 65 days later. An accident investigation committee was convened, root cause analysis was conducted, and measures to prevent recurrence were developed.Conclusion: This adverse event occurred because the protocol for intraoperative blood salvage management was not clearly defined, and the procedure was different from the for standard transfusion practices. There were unexpected events, such as the presence of salvaged blood from other patients as well as inadequate and ambiguous information-sharing among the parties involved. We developed a systemic improvement strategy in which intraoperative blood collection would be administered before the patient leaves the operating room to completely prevent recurrence, instead of simply requiring front-line staff to do double-check. Implementing strong systems processes can reduce the risk of errors in blood transfusions and ensure that patients receive the correct blood products.
Detection of Human Adenovirus among Iranian Pediatric Hospitalized Patients Suspected to COVID-19 Epidemiology and Comparison of Clinical Features
Authors: Mohammadi Mohsen , Bid-Hendi Shadi , Baghershiroodi Mahnaz , Chehrazi Mohammad , Yahyapour Yousef , GouranOurimi Azin , Sadeghi Farzin 
Published: May 2022

Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in children typically results in similar symptoms with other viral respiratory agents including human adenoviruses (HAdVs). Mixed HAdV and SARS-CoV-2 infection (co-infection) in children might result in enhanced or reduced disease severity compared with single infections. The present study aims to investigate the rate of SARS-CoV2 and HAdV infection and also their coinfection and compare the two infections regarding their laboratory, and clinical characteristics at hospital admission. A total of 360 combined oropharyngeal and nasopharyngeal swab samples from hospitalized children were examined.by real-time PCR for the existence of the SARS-CoV-2 and HAdVs The symptoms, clinical characteristics and laboratory findings were retrieved and compared in SARS-CoV-2 and HAdVs positive cases. Of the total 360 suspected COVID-19 hospitalized children, 45 (12.5%) and 19 (5.3%) specimens were PCR-positive for SARS-CoV-2 and HAdV respectively. SARS-CoV-2 and HAdV co-infection was detected in 4 cases (1.1%). Regarding symptoms at hospital admission, fever in SARS-CoV-2 positive group were significantly higher than those in HAdV positive group [34 (85%) vs. 7 (46.7%), p = 0.012]. However, percentages of cases with sore throat, headache, fatigue, lymphadenopathy and conjunctivitis in HAdV positive group were significantly higher than those in SARS-CoV-2 positive group. SARS-CoV-2 and HAdV co-infected children showed mild respiratory symptoms. The present study revealed that SARS-CoV-2 positive children often appear to have a milder clinical course than children with respiratory HAdV infection and children co-infected with SARSCoV-2 and HAdV had less-severe disease on presentation.
Circular RNA circFIRRE drives osteosarcoma progression and metastasis through tumorigenic-angiogenic coupling
Authors: Yu Lingfeng , Zhu Hao , Wang Zhen , Huang Jianhao , Zhu Yan , Fan Gentao , Wang Yicun , Zhou Guangxin 
Published: May 2022

Abstract
Background
Disappointing clinical efficacy of standard treatment has been proven in refractory metastatic osteosarcoma, and the emerging anti-angiogenic regimens are still in the infantile stage. Thus, there is an urgent need to develop novel therapeutic approach for osteosarcoma lung metastasis.
Methods
circFIRRE was selected from RNA-sequencing of 4 matched osteosarcoma and adjacent samples. The expression of circFIRRE was verified in clinical osteosarcoma samples and cell lines via quantitative real-time polymerase chain reaction (RT-qPCR). The effect of circFIRRE was investigated in cell lines in vitro models, ex vivo models and in vivo xenograft tumor models, including proliferation, invasion, migration, metastasis and angiogenesis. Signaling regulatory mechanism was evaluated by RT-qPCR, Western blot, RNA pull-down and dual-luciferase reporter assays.
Results
In this article, a novel circular RNA, circFIRRE (hsa_circ_0001944) was screened out and identified from RNA-sequencing, and was upregulated in both osteosarcoma cell lines and tissues. Clinically, aberrantly upregulated circFIRRE portended higher metastatic risk and worse prognosis in osteosarcoma patients. Functionally, in vitro, ex vivo and in vivo experiments demonstrated that circFIRRE could drive primary osteosarcoma progression and lung metastasis by inducing both tumor cells and blood vessels, we call as “tumorigenic-angiogenic coupling”. Mechanistically, upregulated circFIRRE was induced by transcription factor YY1, and partially boosted the mRNA and protein level of LUZP1 by sponging miR-486-3p and miR-1225-5p.
Conclusions
We identified circFIRRE as a master regulator in the tumorigenesis and angiogenesis of osteosarcoma, which could be purposed as a novel prognostic biomarker and therapeutic target for refractory osteosarcoma.
Antibacterial activities, chemical composition, and efficacy of green extract Carica papaya peel on food model systems
Authors: Asri Nurul Aimi Amanina Mohamad , Sani Muhamad Shirwan Abdullah , Othman Rashidi , Nordin Noor Faizul Hadry , Desa Mohd Nasir Mohd 
Published: May 2022

Abstract
This study investigated antibacterial activities, chemical composition and extract efficacy of Carica papaya peel (CPPE) var. Sekaki/ Hong Kong. Nine green solvents were used to extract the Carica papaya peel, and the extracts were subjected to antibacterial tests and assays against 14 bacteria. The most potent extract was then subjected to phenolic and flavonoid assays, gas chromatography-mass spectrometry (GC/MS) analysis, and efficacy study on food model systems. All CPPEs showed antibacterial activities, and pentane extract had moderate to clear inhibitions against all 14 bacteria. Ethanol extract of Carica papaya peel (ECPPE) inhibited C. perfringens, L. monocytogenes, B. subtilis, V. parahaemolyticus, and V. vulnificus with a minimum inhibitory concentration (MIC) of 1.563 mg/ml; therefore, the ECPPE was selected as the most potent extract. The total phenolic (TPC) and flavonoid contents (TFC) of the CPPEs ranged between 6.20 to 58.75 mg GAE/g DW and 1.35–29.09 mg QE/g DW, respectively. Palmitic acid, linoleic acid, β-sitosterol and stigmasterol in ECPPE may be potential antibacterial compounds that render antibacterial activities. The ECPPE could effectively inhibit the tested bacteria in low carbohydrate and high protein, fat, and fibre food model systems, which may suit the investigation of ECPPE efficacy in meat, poultry and dairy products, high fat-content products such as mayonnaise, butter and margarine and high fibre food such as legumes, lettuce and barley.
Fabrication and Electrical Characterization of a Transistor Device Configuration Based on Graph
Authors: Osorio Humberto Franco 
Published: May 2022

Abstract
The films of graphene oxide multilayer synthesized from bamboo as material source (GO), by the method of double thermal decomposition (DTD-method), for carbonization temperatures of 973 and 1023 K, exhibited electrical behavior as semiconductor with narrow band-gap energy and conduction mechanism by the Mott 3D variable range hopping, postulating this material as an excellent candidate for the development of electronic devices. This work presents the fabrication procedure and electrical characterization of a transistor configuration based on GO films (GOT). The electrical characterization was carried out in an automated system at low power and it were measured the transistor curves families of input, output, reaction and transfer in GOT devices. The results obtained suggest that GOT devices could be used in switching and voltage attenuation applications in low electrical power circuits, given the electrical behavior obtained as voltage dependent voltage sources with unitary gain approximately.
The Immunoreactivity Patterns of BRAF VE1 and its Diagnostic Value in Brain Tumors
Authors: Li Hainan , Fan Chongzhu , Liu Minting , Chen Wendan , Cheng Baijie , Xiang Xuan , Zhang Zhenbin , Cai Linbo , Li Zhi 
Published: May 2022

Abstract
Background
BRAF-V600E mutation is considered as a material biomarker with diagnosis, prognosis and prediction in brain tumors. At present, molecular detection remains the most responsible approach for BRAF V600E mutation. The accuracy of Immunohistochemical test for Anti-BRAF VE1 antibody has rarely been systematically evaluated in brain tumors, despite it is widely used in pathological diagnostic practice.
Methods
A total of 120 brain tumors were detected by immunohistochemical staining and real-time qPCR for BRAF V600E mutation. These cases were divided into 4 groups by Immunoreactivity Patterns under a microscope, including: diffuse positivity, reliable positivity of single tumor cells, uniform "coating" positivity and Negative. Then the Image-Pro Plus 6.0 image processing and analysis software were used to measure the stained area, integrating absorbance(IA)of stained area. The accuracy of immunohistochemical detection was verified by real-time qPCR. The BRAFV600E mutation prediction model was further established using Bayesian classification with tumor type, area of positive and average IA of stained area. All statistical analyses were performed in R (version R 3.6.3)
Result
The sensitivity of immunohistochemical detection was 97.01% and the specificity was 100%(P < 0.0001). The BRAFV600E mutation prediction model was further established with a AUC value of 0.95.
Conclusions
BRAF VE1 staining could replace molecular detection to some extent, on the premise of mastering the key points in the interpretation of BRAF VE1 immunostaining. Otherwise, the prediction model of BRAF V600E mutation may provide a possible scheme for artificial intelligence interpreting of immunohistochemical staining of BRAF VE1.
Ovarian gonadoblastoma with the karyotype of 46, XX: A case report
Authors: Zhao Fengju , Zhao Yingchao , Xing Biyuan , Liu Zhao 
Published: May 2022

Abstract
Gonadoblastoma is a rare tumor comprised of sex cord derivatives and germ cells. The risk for developing gonadoblastoma increases significantly in patients who possess a Y chromosome or Y chromosome material. A 49-year-old Chinese woman found a pelvic mass during a routine physical examination. Pathological analysis after surgery indicated that the tumor was unilateral ovarian gonadoblastoma with dysgerminoma. Compared with other cases in the literature, our patient was the oldest, and the tumor mass was smaller. Karyotype analysis of peripheral blood lymphocytes revealed that the woman had a 46, XX female karyotype. Whole-exon sequencing revealed that some mutations, such as altered somatic genes in the Forkhead box protein O (FoxO) signaling pathway and KIT, might cause the disease. In conclusion, we described a rare case of gonadoblastoma in a woman who had normal routine menstruation, sexual development, and successful pregnancies and possessed a normal female 46, XX karyotype.
Parques Tecnológicos: Uma Análise das Publicações da Última Década
Authors: Bresciani Sirlene Aparecida Takeda , Souza Duarte Miranda Francieli , Teixeira Clarissa Stefani , Johann Denise Adriana , Biz Alexandre Augusto 
Published: May 2022

O tema Parques Tecnológicos tem ganhado popularidade por se tratar de ambientes promotores da cultura de inovação na produção de conhecimento tecnológico, que contribuem para o desenvolvimento econômico e social das regiões onde atuam. Diante disso, o objetivo desse estudo foi demonstrar o panorama das publicações sobre parque tecnológico na coleção de base de dados Web of Science e Scopus, no período de 2010 a 2020. Para tanto, utilizou-se a técnica bibliometrica, com o tópico “technological park”, sendo encontrados o total de 133 publicações, as quais 42 encontram-se disponíveis na WoS e 91 na base Scopus. Os dados encontrados identificaram que a maior frequência de publicações foi no ano de 2018, com o total de 26. Os autores que mais publicaram foram Nagano e Vick. O país com maior frequência foi o Brasil, com 52 publicações. O idioma é inglês e o tipo de documento é artigo científico. Já, a área de pesquisa é Engenharia e a instituição é a Universidade de São Paulo. Em relação à quantidade de citações, tem-se o total de 174. Após, gerou-se um mapa de clusters de cocitações e de palavras-chave. No decorrer do trabalho, foi possível verificar a importância e utilidade do uso da técnica bibliométrica para análise de dados disponíveis em bases. Quanto às limitações, nesse estudo não foram filtrados estudos duplicados em ambas as bases, portanto, sugere-se que futuros estudos façam essa tarefa e, portanto, recomenda-se realizar um estudo sistemático e a construção de uma agenda de pesquisa.
Forecasting water quality using seasonal ARIMA model by integrating in-situ measurements and remote sensing techniques in Krishnagiri reservoir, India
Authors: Abdul Wahid A. , Arunbabu E. 
Published: May 2022

Abstract
The Krishnagiri reservoir is the main source of irrigation in Tamil Nadu, India. It has been reported to be hypereutrophic for over a decade with sediment and nutrient load sources responsible for the degradation of water quality. Remotely sensed satellite imagery analysis plays a significant role in assessing the water quality for developing a management strategy for reservoirs. The present study is an attempt to demonstrate the improvement in the chlorophyll-a (chl-a) estimation in the Krishnagiri reservoir by integrating remote sensing and in-situ measurements. Multiple regression equations were developed with the reflectance of Green, Red, NIR and SWIR1 bands of the Operational Land Imager (OLI) sensor of Landsat 8 satellite yielded the coefficient of determination for chlorophyll-a (chl-a) as 0.812, total dissolved solids (TDS) as 0.945 and electrical conductivity (EC) as 0.960 respectively. The developed regression model was further utilised to forecast chl-a and EC of the reservoir through the seasonal auto regressive integrated moving average (SARIMA) model. It is found that chl-a prediction showed that the reservoir continued to be hypereutrophic and EC significantly changed from a class C3 (high salinity) to class C4 (very high salinity). These results are alarming and an immediate reduction of the external load from the catchment through effective watershed management programs should be implemented.