bluuebunny/crossref_metadata_2025_split huggingface dataset:
The cortisol awakening response reflects fatigue caused by high-intensity resistance exercise
Authors: Ogasawara Yui , Yoneyama Takuma , Ikuji Takeaki , Tsuchiya Hironobu , Sugo Takayuki 
Published: April 2022

Abstract
Background
The cortisol awakening response (CAR) reflects the physical adaptation to exercise and training loads and has been reported the response especially by high-intensity exercise. However, few studies have examined the effects of resistance exercise. The purpose of this study was to investigate the effect of high-intensity resistance exercise on the CAR.
Methods
Thirteen healthy male university students performed 1 repetition maximum (1 RM) tests and an experimental session for 4 consecutive days: sedentary control sessions (Day 1 and 2), high-intensity resistance exercise (bench press) session at 75% of 1 RM (HIRE; Day 3), and recovery session (Day 4). Saliva samples for Day 1–3 were collected: 1) before and 2) immediately after the session, 3) 10 min, 4) 20 min, and 5) 30 min into the recovery period from each session, 6) 9 pm, and 7) 11 pm at night after the session, and 8) immediately after awakening and 9) 15 min and 10) 30 min post-awakening the day after the session (Sampling on Day 4 only upon awakening).
Results
The results showed that the HIRE session did not change the acute cortisol levels; however, the CAR showed significantly different responses in those who increased (n = 7; Responders) and those who did not increase (n = 6; non-Responders). In addition, a significant negative correlation was found between the rate of increase in area under the curve for the whole of CAR (AUCg) and the amount of change of physical fatigue from control days (Day 1 and 2) to Day 3 and 4 (r = − .478, p = .014).
Conclusions
These results suggest that both increased and no increased CAR may result from physical adaptation and maladaptation to the HIRE session. Therefore, CAR has the potential to assess the physical adaptation to the loads of local resistance exercise as well, which may help to plan future training loads and recovery.
Chemotherapy-induced neutropenia as a prognostic factor in patients with extensive-stage small cell lung cancer
Authors: Miyagi Takehiro , Tsuji Daiki , Kawasakai Yohei , Ishikawa Hiroshi , Tanaka Rei , Nakao Masahiko , Nakagaki Shigeru , Hayashi Toshinobu , Ayuhara Hideaki , Harada Tomohiko , Tamaki Shinya , Maeda Akimitsu , Ohashi Yasukata , Arakawa Yuichiro , Fujita Yukiyoshi , Yamamoto Keisuke , Miyamoto Yasunori , Yano Takuya , Itoh Kunihiko 
Published: April 2022

Abstract
Chemotherapy-induced neutropenia (CIN) is a dose-limiting factor for cytotoxic chemotherapy, but recently, it was suggested that CIN contributes to prolonged survival. In this study, we examined the association between severe CIN and survival, and determined whether CIN affected survival in patients with extensive-stage small cell lung cancer (ED-SCLC).The medical records from 214 patients with ED-SCLC treated with etoposide or irinotecan in combination with cisplatin (EP/IP) between 2012 and 2016 were collected and retrospectively analyzed. Landmark analysis was performed at the end of cycle 4 and the relationship between severe CIN and survival was determined by a log-rank test. In addition, a multivariate analysis using COX proportional hazard model was performed to identify independent predictive factors. The Landmark analysis included 102 patients in the IP-group and 47 patients in the EP-group. No significant difference was found between grade 0–3 and grade 4 neutropenia and overall survival (OS) in the EP-group. (P = 0.57). Contrariwise, for the IP patients, the median OS was 444 days for grade 0–3 and 633 days for grade 4 neutropenia, which was significantly longer for patients who developed grade 4 neutropenia (P = 0.03). Multivariate analysis adjusted for potential factors revealed that the development of grade 4 CIN was identified as a significant predictor of longer OS (hazard ratio [HR], 0.46; 95% confidence interval (CI), 0.26–0.81, P = 0.008). The results indicated that the development of severe CIN with IP therapy is associated with prolonged OS.
Relationship between seminal HIV-1 RNA shedding and genital schistosomiasis in HIV-positive men receiving antiretroviral therapy (ART) along the south shoreline of Lake Malawi: A prospective pilot study
Authors: Kayuni Sekeleghe A. , Abdullahi Adam , Alharbi Mohammad H. , Makaula Peter , Lampiao Fanuel , Juziwelo Lazarus , LaCourse E. James , Kumwenda Johnstone J. , Leutscher Peter D.C. , Geretti Anna Maria , Stothard J. Russell 
Published: April 2022

Abstract
Background: Male Genital Schistosomiasis (MGS) is hypothesized to increase seminal shedding of HIV-1 RNA. We aimed to assess the impact of MGS on genital HIV-1 RNA shedding by longitudinally measuring HIV-1 RNA levels in men established on long-term ART comparing those with and without a diagnosis of MGS. Methods: Following optimisation of the GeneXpert® HIV-1 RNA assay for use with seminal fluid, we analysed 76 paired plasma and semen samples collected from 31 men over 12 months, comprising 15 with and 16 subjects without MGS. Study visits occurred at 0, 1, 3, 6 and 12 months. A diagnosis of schistosomiasis was based on Schistosoma positivity using eggs by microscopy of filtrated urine, semen, seminal sediment, POC-CCA test or by testing PCR of seminal sediment or urine at one or more time points. MGS was diagnosed by egg positivity on semen microscopy or PCR of seminal sediment. All subjects were blindly treated with a single dose of praziquantel at each study visit.Results: Across the entire study population, HIV-1 RNA was detected in 7/76 (9.2%) seminal samples and 29/76 (38.2%) plasma samples. Among all participants, 5/31 (16.1%) showed HIV-1 RNA detection in one or more seminal samples, with 3/5 (60%) showing HIV-1 RNA detection in semen only with levels up to 400 copies/ml. The three subjects were in the MGS positive group. The two subjects showing HIV-1 RNA in seminal fluid from the MGS negative group also had concomitant HIV-1 RNA detection in plasma. Conclusion: We found no notable difference in the pattern of genital shedding in the seminal compartment of HIV-1 infected men established on ART with and without a diagnosis for MGS following treatment with praziquantel.
Curtobacterium allii sp. nov., the actinobacterial pathogen causing onion bulb rot
Authors: Khanal Manzeal , Bhatta Bed Prakash , Timilsina Sujan , Ghimire Sudeep , Cochran Kimberly , Malla Subas 
Published: April 2022

Abstract
A Gram positive, aerobic, and non-spore-forming bacterial strain, 20TX0166T, was isolated from a diseased onion bulb in Texas, USA. Upon testing its pathogenicity on onion bulb, it produced pathogenic response which makes it first species of pathogen belonging to the phylum actinobacteria detected in onion. Phylogenetic analysis of the 16S rRNA gene sequence revealed that the strain belonged to the genus Curtobacterium and was most similar to Curtobacterium flaccumfaciens LMG 3645T (100%), C. pusillum DSM 20527T (99.5%), and C. oceanosedimentum ATCC 31317T (99.5%). The orthologous ANI (orthoANIu), ANI based on blast (ANIb), and dDDH values between the novel strain and the closest relative, C. flaccumfaciens LMG 3645T, were 95.7%, 95.4%, and 63.3%, respectively. These values were below the recommended species cut-off threshold of 96% (ANI) and 70% (dDDH), suggesting the strain may be a novel species. The estimated genome size of the novel species was 3.98 Mbp with a G + C content of 70.8%. Physiologic and phenotypic characters of this novel strain were also unique when compared with the closely related species. The major cellular fatty acids of this strain were C15:0 anteiso and C17:0 anteiso. Using a polyphasic approach based on phenotypic and genotypic analyses, strain 20TX0166T represents a novel species of the genus Curtobacterium, and the name Curtobacterium allii sp. nov. is proposed. The type strain is 20TX0166T (= LMG 32517T, =CIP112023T, =NCIMB 15427T).
DIA-based Proteomics Analysis of Serum-derived Exosomal Proteins as Potential Candidate Biomarkers for Intrahepatic Cholestasis in Pregnancy
Authors: Nie Liju , Xin Siming , Zheng Jiusheng , Luo Yong , Zou Yang , Liu Xianxian , Chen Huayan , Lei Xiaozhen , Zeng Xiaoming , Lai Hua 
Published: April 2022

Abstract
Background Data-independent acquisition (DIA) is one of the most powerful and reproducible proteomic technologies for large-scale digital qualitative and quantitative research. The aim of this study was to use proteomic methodologies for the identification of biomarkers that are over- or underexpressed in women with intrahepatic cholestasis of pregnancy (ICP) compared with controls and discover a potential biomarker panel for ICP detection.Methods The participants included 11 ICP patients and 11 healthy pregnant women as controls. The clinical characteristic data and the laboratory biochemical data were collected at the time of recruitment. Then, a data-independent acquisition (DIA)-based proteomics approach was used to identify differentially expressed proteins (DEPs) in serum exosomes between ICP patients and controls. Finally, bioinformatics analysis was used to identify the relevant processes in which these DEPs were involved. Results The proteomics results showed that there were 162 DEPs in serum exosomes between pregnant women with ICP and healthy pregnant women, of which 106 were upregulated and 56 were downregulated in ICP. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the identified proteins were functionally related to specific cell processes including apoptosis, lipid metabolism, immune response and cell proliferation, and metabolic disorders, suggesting that these may be primary causative factors in ICP pathogenesis. Meanwhile, complement and coagulation cascades may be closely related to the development of ICP. Receiver operating characteristic curve (ROC) analysis showed that the area under the curve values of Elongation factor 1-alpha 1, Beta-2-glycoprotein I, Zinc finger protein 238, CP protein and Ficolin-3 were all approximately 0.9, indicating the promising diagnostic value of these proteins.Conclusions This preliminary work provides a better understanding of the proteomic alterations in the serum exosomes of pregnant women with ICP and may provide new insights into ICP pathophysiology and potential novel treatment targets for this disease. However, future studies of the systemic expression of these candidate biomarkers and their exact roles in ICP will be essential for conformation of this hypothesis.
Squamous cell carcinoma transformed from the prostatic adenocarcinoma with hormonotherapy: a case report and literature review
Authors: Song Shizhang , Jiang Bo , Hou Sichuan , Huang Xingang , Li Chunmei , Liu Zhijun 
Published: April 2022

Abstract
Background: Squamous cell carcinoma (SCC) of the prostate is a very rare and highly aggressive tumor, which is ineffective to multiple treatments and prone to metastasis, and has a worse prognosis than adenocarcinoma of the prostate. However, a transformation of prostatic adenocarcinoma into squamous cell carcinoma is rarer and may occur after hormonotherapy or radiotherapy. Hitherto, there are few cases in the world about the transformation from adenocarcinoma into squamous cell carcinoma after treatment. To our knowledge, our case is the first reported in China.Case presentation: A 67-year-old man with metastatic adenocarcinoma of the prostate for 2 years was not suitable for radical prostatectomy due to the disease classified T4N1M1. Hormonotherapy using Luteinizing Hormone-Releasing Hormone (LHRH) analog (leuprorelin) and antiandrogen agent (bicalutamide) was started, and serum prostate-specific antigen (PSA) level gradually decreased to a nadir of 0.04ng/ml. After treatment of 2 years, he complained of worsening of lower urinary tract symptoms(LUTS), and then he underwent transurethral resection of the prostate (TURP). Histological analysis revealed there were poorly differentiated keratinizing squamous cell carcinoma in the most of cancer tissue. As of now, the patient has undergone docetaxel treatment, containing 2 times of systemic chemotherapy. The patient's general condition is currently good.Conclusions: Prostatic adenocarcinoma transformed into squamous cell carcinoma after hormonotherapy is very rare. However, the serum PSA of this tumor is probably normal, PSA and the Gleason grading system are of limited value in the diagnosis of SCC, keratinization of histopathological examination enables its diagnosis. The transformation is subclinical. Hence, how to identify prostatic adenocarcinoma or SCC in prostate tissue needs a further investigation.
The evaluation of honey bee venom potential therapeutic value on gonadotropin-induced ovarian hyperstimulation syndrome model rats
Authors: Nabiuni Mohammad , Karimzadeh Latifeh 
Published: April 2022

Abstract
Ovarian hyperstimulation syndrome (OHSS) mostly occurs in undergoing gonadotropin therapy women sensitive to endogenous gonadotropins. Honey bee venom (HBV) contains biologically active components that has pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory and anti-angiopathy therapeutic agent to suppress main inflammatory mediators in OHSS. OHSS induced by Pregnant Mare’s Serum Gonadotropin) PMSG) and hCG intraperitoneal in immature female Wistar rats. OHSS induction followed by intraperitoneal injection of HBV and metformin for 10 consecutive days;. At the end of the treatment period, metabolic parameters as well as ovarian expression of IL-6, COX2, and VEGF proteins were evaluated. This data showed that the OHSS+HBV group had a significant reduction in peritoneal and ovarian vascular permeability compared to the OHSS group. The ovarian expression of COX2, IL-6 and VEGF proteins in group OHSS+HBV.. The results of this study showed that HBV can affect the inflammatory feature of OHSS by reducing the angiogenesis factors.
LncRNA FOXD3-AS1 enhances the migration and invasion ability of breast cancer cells through the Wnt/β-Catenin signaling pathway
Authors: Tao Bijun , Wu Yueling , Liu Jiande , Jia Zhe , Xiong Zhenquan , Zhang Lihua , Zhang Wanting , Yang Jing , Wang Yuzhou 
Published: April 2022

Abstract
Objective
To explore the expression, biological function, and mechanism of action of the LncRNA FOXD3-AS1 in breast cancer.
Methods
The expression of LncRNA FOXD3-AS1 was analyzed in the TCGA database using bioinformatics analysis. Moreover, the relative expression of LncRNA FOXD3-AS1 was measured in the human normal mammary epithelial cell line (MCF10A) and three breast cancer cell lines (BT-549, MDA-MB-231, and MCF-7). After siRNA knockdown of FOXD3-AS1 expression in BT-549, MDA-MB-231 cells, alterations in cell proliferation, migration, invasion, and apoptotic capacity were determined by CCK-8, scratch assay, transwell assay, and Annexin V-FITC/PI staining, respectively. Furthermore, changes in the level of the β-Catenin protein were detected using western blot.
Results
TCGA database analysis results relative to adjacent tissues revealed that the expression of LncRNA FOXD3-AS1 was markedly elevated in breast cancer tissues. Besides, results of real-time PCR established that the relative expression of FOXD3-AS1 was significantly higher in the breast cancer cell lines (BT-549, MDA-MB-231, and MCF-7) than in the normal human mammary epithelial cell line (MCF10A) (P < 0.0001). Inhibition of FOXD3-AS1 expression by siRNA significantly inhibited the wound healing ability, migration and invasion ability of BT-549 and MDA-MB-231 cells. However, there was no difference in proliferation and apoptosis. The western blot results showed that inhibition of FOXD3-AS1 expression by siRNA significantly inhibited the level of the β-Catenin protein in BT-549 and MDA-MB-231 cells.
Conclusion
The abnormally high expression of LncRNA FOXD3-AS1 in breast cancer may be related to disease progression. LncRNA FOXD3-AS1 can significantly enhance the migration and invasion ability of breast cancer cells, and its mechanism of action may be related to the Wnt/ β-Catenin signaling pathway.
Evaluation of the Predictive Value of Echocardiography Parameters for Heart Transplant Rejection: A Tissue Doppler Imaging Study
Authors: Keihanian Faeze , Ghaderi Fereshteh , Poorzand Hoorak , Vakilian Farveh , Alimi Hedieh , Bigdelu Laila , Fazlinezhad Afsoon , Rafighdoost Amirhossein 
Published: April 2022

Abstract
Background: The rate of heart transplantation is increasing worldwide. Due to the drawbacks of endomyocardial biopsy (EMB), various non-invasive methods have been suggested to assess rejection. Therefore, the aim of this study was to evaluate the predictive value of echocardiographic parameters to determine heart transplant rejection in a sample of Iranian patients. Methods: This was a cross-sectional study on heart transplant patients with available EMB results. All patients underwent echocardiography at the same day of EMB and prior to biopsy. The association between echocardiographic parameters and rejection was assessed using binary logistic regression. Results: A total of 67 patients (50, 74.6% male and 17, 25.4% female) with the mean age of 39.20 ± 11.39 years entered this study. Heart transplant rejection was observed in 22 (32.8%) patients. There was only a significant difference in mitral inflow early diastolic flow (E) and septal time to peak systolic velocity in ejection phase (septal Ts) between rejection and non-rejection groups. Logistic regression revealed a significant association between rejection and septal Ts (p=0.048, OR=0.931) and E velocity (p=0.022, OR=78.069). Based on ROC curve, the area under the curve for septal Ts and E were 81.9% and 68.6%. Moreover, the sensitivity and specificity for septal Ts and E in detection of rejection were 75%, 69% and 68%, 61%, respectively. Conclusion: Septal Ts could be used as a good echocardiographic parameter for predicting rejection in heart recipient patients.
Fe₃O₄@Polydiallyl Isophthalate Magnetic Microspheres for Enhanced MRI/CT and Magnetothermal Effect-Guided Tumor Ablation
Authors: Chen Piaoyi , Zhou Yanfang , Chen Mianrong , Lun Yingying , Li Qiuxia , Xiao Qinglin , Huang Yugang , Li Jiesong , Ye Guodong 
Published: April 2022

Abstract
Background: Multi-functional embolic microspheres for treating stage B hepatocellar carcinoma often require complex synthesis processes and harsh conditions. But the traditional embolic agents (e.g. polyvinyl alcohol and iodized oil) often cause unexpected embolism and have potential risks of damaging the human body. The aim of this research was to use a photo-click method to prepare multifunctional microspheres with magnetocaloric effect and MR/CT/DSA imaging function used in the transcatheter arterial embolization.Methods: Fe3O4@polydiallyl isophthalate (Fe3O4@PDAIP) magnetic microspheres were synthesized by one-pot photopolymerization without hindered by degradative chain transfer. The in vitro and in vivo anti-tumor effects were characterized with hepatoma cell experiments and tumor-bearing mouse model. The DSA and CT in vitro were utilized to evaluate imaging ability of Fe3O4@PDAIP. The safe was tested via histopathological examination and serum detection.Result: The results showed that Fe3O4@PDAIP magnetic microspheres had ablation effect on tumor cells and had imaging effect through MR/CT/DSA scanning. The microspheres was heated to 42-48 oC by magnetocaloric effect in alternating magnetic field, which significantly decreased the activity of mouse liver cancer cells in vitro. In vivo, it can inhibit the growth of tumor after magnetothermal therapy without obvious toxicity.Conclusion: Fe3O4@PDAIP magnetic microspheres can be potentially applied to the locoregional and precise interventional treatment of liver tumors.
Weight loss in elderly orthopaedic patients leads to bone loss
Authors: Chen Xiangxu , Bai Liyong , Tian Chuwei , Zhao Yakuan , Zhang Cheng , Shi Liu , Zhang Yuanwei , Xie Wenjun , Chen Hui , Rui Yunfeng 
Published: April 2022

Abstract
BackgroundThe number of patients with osteoporosis or low bone mass is increasing annually. Weight loss is reportedly associated with bone loss and is a strong predictor of osteoporosis. It was still not clear the relationship between weight loss and bone loss in the elder.MethodsThe study included 520 patients aged ≥65 years (178 men and 342 women). Age, gender, weight, and height were recorded. Femoral neck bone mineral density and T-score were investigated using a dual-energy X-ray absorptiometry scanner. Blood calcium (Ca), phosphorus (P), albumin (ALB), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) levels were measured. Patients were classified by gender (male and female), age (65-79 years and ≥80 years), and T-score: normal, osteopenia and osteoporosis.ResultsAge, gender, body mass index (BMI), ALP and TG were independent factors for osteoporosis. For the 65-79 and ≥80 year age groups, female patients presented lower T-scores than males. Ca, P, ALB, ALP, TC, HDL and LDL levels were significantly different between men and women in the 65-79 year age group. In addition, BMI and TG levels were significantly decreased in osteoporotic patients compared with normal bone mass patients. TC levels declined in 65-79 year-old male and female patients with osteoporosis. In the group of women aged ≥80 years, osteoporotic patients showed significantly increased ALP levels. Furthermore, we found positive correlations between BMI and TG levels in the groups of male and female patients. However, we found no significant differences in ALB, Ca, P, HDL and LDL levels in osteoporotic compared to normal bone mass patients.Conclusion Osteoporotic patients showed significantly decreased BMI and TG levels compared with those with normal bone mass. BMI was showed positive correlations with TG levels in male and female patients. These results indicate a correlation between weight loss and bone loss and a correlation between lipid profiles and bone mass.
Brain Computer Interface using hybrid adaptive filtering and Higher order Crossing Analysis based on Modified DNN
Authors: Sharad Shwetav , Chakraverti Sugandha , Kundu Shakti , Chakraverti Ashish Kumar , Singh Murari Kumar 
Published: April 2022

Abstract
Brain computer interface (BCIs) aims to communicate with external devices and also recognize human activities through brain signals. However, existing algorithms have some drawbacks, such as poor resolution, high-frequency noise, risk, and low accuracy. Various machine learning algorithms have been used to recognize human activities, but it is more complex to recognize the activities of humans. In order to solve these issues BCIs system based on modified DNN is proposed. Using a modified DNN algorithm, all human activities are recognized through the brain signals. The proposed method contains two phases such as training and testing the data. First, the Brain signal is taken as input, and then the next phase is pre-processing. The signal is pre-processed using a bandpass filter to remove noise present in the signal. Then the next phase is feature extraction, for that features are extracted from the pre-processed signal using Hybrid Adaptive Filtering (HAF) and Higher Order Crossing (HOC) analysis. In order to evaluate the HAF-HOC performance on a user-independent basis, a set of brain signals for each human activity was analyzed and pre-processed. The next phase is classification. For classifying the brain signal, a modified DNN algorithm is used. The Sailfish Optimization technique is used to train the neural network and also used to optimize the weights of the DNN classifier. The results show that the proposed method can obtain 98% accuracy and 0.03 Error, and 10% False Positive Rate. An existing system such as SVM, KNN, RF, and NB reached 86%, 90%, 83% and 70% accuracy, respectively, which is lower than our proposed method.