bluuebunny/crossref_metadata_2025_split huggingface dataset:
Achieving quantitative and accurate measurement of the human gut microbiome
Authors: Maghini Dylan , Dvorak Mai , Dahlen Alex , Roos Morgan , Kuersten Scott , Bhatt Ami S. 
Published: September 2022

AbstractRobust benchmarking studies have highlighted how measured relative microbial abundances can vary dramatically depending on how DNA is extracted, made into libraries, sequenced, and analyzed. To build upon prior research, we investigated how sample preservation and storage choices impact observed absolute microbial load and relative metagenomic and metatranscriptomic measurements. Specifically, we studied how two common stool preservatives (OMNIgene GUT OMR200 and Zymo DNA/RNA PowerShield) perform across a range of storage temperatures (−80°C, 23°C and 40°C). For immediately frozen samples with no preservatives, we observed a mean colonic load of ∼100 trillion (1.2 × 1014) prokaryotes across ten donors, revising the gut prokaryote:human cell ratio of ∼1:1 to ∼4:1. We found that both preservatives introduce significant bias in the metagenomics results; and, while OMNIgene results were robust to storage temperature, samples stored in Zymo preservative had further bias with increasing storage temperatures. In terms of measured composition, we observed a ∼1.9x and ∼1.5x difference in the metagenomic Bacteroidetes:Firmicutes ratio in OMNIgene and Zymo preservatives, respectively. Absolute abundance measurements revealed that these differences are driven by higher measured Bacteroidetes in OMNIgene-preserved samples and lower measured Firmicutes in Zymo-preserved samples. For metatranscriptomic measurements, we also found that both preservatives introduced bias, but that RNA likely degraded in samples stored in OMNIgene preservative at high temperature. In summary, we recommend the OMNIgene preservative for studies that include significant field components. For metatranscriptomics studies, we recommend kits rated for RNA preservation such as the Zymo kit; however, existing samples collected in non-RNA rated kits might also be viable for limited metatranscriptomic studies. This study demonstrates how sample collection and storage choices can affect measured microbiome research outcomes, makes additional concrete suggestions for sample handling best practices, and demonstrates the importance of including absolute abundance measurements in microbiome studies.
De novo protein identification in mammalian sperm using high-resolution in situ cryo-electron tomography
Authors: Chen Zhen , Shiozaki Momoko , Haas Kelsey M. , Zhao Shumei , Guo Caiying , Polacco Benjamin J. , Yu Zhiheng , Krogan Nevan J. , Kaake Robyn M. , Vale Ronald D. , Agard David A. 
Published: September 2022

AbstractUnderstanding molecular mechanisms of cellular pathways requires knowledge of the identities of participating proteins, their cellular localization and their 3D structures. Contemporary workflows typically require multiple techniques to identify target proteins, track their localization using fluorescence microscopy, followed by in vitro structure determination. To identify mammal-specific sperm proteins and understand their functions, we developed a visual proteomics workflow to directly address these challenges. Our in situ cryo-electron tomography and subtomogram averaging provided 6.0 Å resolution reconstructions of axonemal microtubules and their associated proteins. The well-resolved secondary and tertiary structures allowed us to computationally match, in an unbiased manner, novel densities in our 3D reconstruction maps with 21,615 AlphaFold2-predicted protein models of the mouse proteome. We identified Tektin 5, CCDC105 and SPACA9 as novel microtubule inner proteins that form an extensive network crosslinking the lumen of microtubule and existing proteins. Additional biochemical and mass spectrometry analyses helped validate potential candidates. The novel axonemal sperm structures identified by this approach form an extensive interaction network within the lumen of microtubules, suggesting they have a role in the mechanical and elastic properties of the microtubule filaments required for the vigorous beating motions of flagella.
USP22 overexpression fails to augment tumor formation in MMTV-ERBB2 mice but loss of function impacts MMTV promoter activity
Authors: Kuang Xianghong , Salinger Andrew , Benavides Fernando , Muller William J. , Dent Sharon Y. R. , Koutelou Evangelia 
Published: September 2022

AbstractThe Ubiquitin Specific Peptidase 22 (USP22), a component of the Spt-Ada-Gcn5 Acetyltransferase (SAGA) histone modifying complex, is overexpressed in multiple human cancers, but how USP22 impacts tumorigenesis is not clear. We reported previously thatUsp22loss in mice impacts execution of several signaling pathways driven by growth factor receptors such as erythroblastic oncogene B b2 (ERBB2). To determine whether changes in USP22 expression affects ERBB2-driven tumorigenesis, we introduced conditional overexpression or deletion alleles ofUsp22into mice bearing the Mouse mammary tumor virus-Neu-Ires-Cre (MMTV-NIC) transgene, which drives both rat ERBB2/NEU expression and Cre recombinase activity from the MMTV promoter resulting in mammary tumor formation. We found that USP22 overexpression in mammary glands did not further enhance primary tumorigenesis in MMTV-NIC female mice, but increased lung metastases were observed. However, deletion ofUsp22significantly decreased tumor burden and increased survival of MMTV-NIC mice. These effects were associated with markedly decreased levels of bothErbb2mRNA and protein, indicatingUsp22loss impacts MMTV promoter activity.Usp22loss had no impact on ERBB2 expression in other settings, including MCF10A cells bearing a Cytomegalovirus (CMV) - drivenERBB2transgene or in human epidermal growth factor receptor 2 (HER2)+ human SKBR3 and HCC1953 cells. Decreased activity of the MMTV promoter in MMTV-NIC mice correlated with decreased expression of known regulatory factors, including the glucocorticoid receptor (GR), the progesterone receptor (PR), and the chromatin remodeling factor Brahma-related gene-1 (BRG1). Together our findings indicate that increased expression of USP22 does not augment the activity of an activated ERBB2/NEU transgene but impacts of Usp22 loss on tumorigenesis cannot be assessed in this model due to unexpected effects on MMTV-driven Erbb2/Neu expression.
Cortical and subcortical connections change after repetitive transcranial magnetic stimulation therapy in cocaine use disorder and predict clinical outcome
Authors: Rasgado-Toledo Jalil , Issa-Garcia Victor , Alcalá-Lozano Ruth , Garza-Villarreal Eduardo A. , González-Escamilla Gabriel 
Published: September 2022

AbstractBackgroundCocaine use disorder (CUD) is a worldwide public health condition which is suggested to induce pathological changes in macro- and microstructure. Repetitive transcranial magnetic stimulation (rTMS) has gained attention to induce a reduction in CUD symptoms. Here, we sought to elucidate whether rTMS induces changes on white-matter (WM) microstructure in frontostriatal circuits after two weeks of therapy in patients with CUD, and to test whether baseline WM microstructure of the same circuits has an effect on clinical improvement. This study consisted of a 2-week, parallel group, double-blind, randomized controlled clinical trial (acute phase) (sham [n=23] and active [n=27]), in which patients received two daily sessions of rTMS on the left dorsolateral prefrontal cortex (lDLPFC) as an add-on treatment. T1-weighted and HARDI-DWI at baseline and two weeks after served to evaluate WM microstructure. After active rTMS, results showed a significant increase in neurite density compared to sham rTMS in WM-tracts connecting left DLPFC with left and right vmPFC. Similarly, rTMS showed reduction in orientation dispersion in WM tracts connecting left DLPFC with left caudate nucleus, left thalamus and left vmPFC. Results also showed a greater reduction in craving VAS after rTMS when baseline ICVF was low in WM tracts connecting left caudate nucleus with substantia nigra, left pallidum, and left thalamus with substantia nigra and left pallidum. Our results evidence rTMS-induced WM microstructural changes in fronto-striato-thalamic circuits and support its efficacy as a therapeutic tool in the treatment of CUD. Further, individual clinical improvement may rely on the patient’s individual structural connectivity integrity.HighlightsWhite matter microstructural changes between fronto-striato-thalamic regions after 2 weeks of rTMS.Whether rTMS would induce microstructural changes may depend on the baseline integrity of the connections between the striatum, thalamus, and the substantia nigra.Our results highlight rTMS as a potential therapeutic tool in the treatment of CUD, due to its ability to modulate altered brain microstructure.
Fronto-Striatal Dynamic Connectivity is linked to Dopaminergic Motor Response in Parkinson’s Disease
Authors: Hensel Lukas , Seger Aline , Farrher Ezequiel , Bonkhoff Anna K. , Shah N. Jon , Fink Gereon R. , Grefkes Christian , Sommerauer Michael , Doppler Christopher E. J. 
Published: September 2022

AbstractBackgroundDifferences in dopaminergic motor response in Parkinson’s disease (PD) patients are related to specific PD subtypes. An important factor driving dopaminergic response might lie in the temporal dynamics in corticostriatal connections.ObjectivesThe aim of this study is to determine if altered resting-state dynamic functional network connectivity (dFNC) is associated with dopaminergic motor response.MethodsWe assessed static and dFNC in 32 PD patients and 18 healthy controls (HC). Patients were subgrouped according to their dopaminergic motor response as low and high responders using a median split.ResultsPatients featuring high dopaminergic responses spent more time in a regionally more integrated state 1 compared to HC. Furthermore, dFNC between aMCC/dACC (anterior midcingulate cortex/dorsal anterior cingulate cortex) and putamen was lower in low responders during a more segregated state 2 and correlated with dopaminergic motor response.ConclusionsAlterations in temporal dynamics of fronto-striatal connectivity might underlie treatment response in PD.
Food values influencing consumers' decisions in a sub-Saharan African country
Authors: Femi-Oladunni Opeyemi Afolabi , Martínez-Ruiz María Pilar , Ruiz-Palomino Pablo , Muro-Rodríguez Ana Isabel 
Published: September 2022

PurposeGiven food industry manufacturers and retailers' growing economic interest in sub-Saharan Africa (SSA), this research aims to contribute to the understanding of consumer food decisions in this specific geographical area. Thus, the intention is to analyze whether there are significant differences in the appreciation of food values according to certain key demographics of consumers (related to the individual and the context) in one of the largest SSA economies: Nigeria.Design/methodology/approachThe review of the relevant literature enabled us to propose a series of hypotheses regarding potential significant differences in the appreciation of a series of food values (in particular, price, safety, environmental impact, nutrition and weight and measures) according to variables related to the individual and the economic and social context. In order to test the hypotheses, a structured questionnaire was personally administered to 500 Lagos residents between June and July 2020. The questionnaire contained, as well as diverse questions related to the appreciation of food values selected for this study, a group of questions about the consumers' sociodemographic characteristics – at both the individual and context level. The database was analyzed using descriptive statistics and Kruskal–Wallis tests.FindingsThe results show that nutrition and weight and measurement values are the most and least appreciated food values, respectively. However, these values differ across segments determined by the chosen sociodemographic variables.Research limitations/implicationsThe results of this study are preliminary and not a complete representation of the Lagos State population, and, consequently, of the Nigerian population. Additional studies in the same and other countries in SSA are needed to confirm the authors’ findings. The results, however, provide an insight into what the most likely outcomes are. A field survey was used as respondents tend not to answer online surveys spontaneously.Practical implicationsFood manufacturers and retailers should encourage consumer-operator feedback mechanisms to improve product characteristics and development. The variations found across each segment can be capitalized upon for advertising and branding food products.Originality/valueHaving selected a country in SSA for the study is an important contribution, given that few studies have focused on this geographical area.
Islamic mobile banking smart services adoption and use in Jordan
Authors: Yaseen Saad G. , El Qirem Ihab Ali , Dajani Dima 
Published: September 2022

PurposeThe research identifies the predictors of Islamic mobile banking (IMB) smart services adoption and usage in Jordan.Design/methodology/approachBased on the Unified Theory of the Acceptance and Use of Technology (UTAUT) and the Unified Theory of the Acceptance and Use of Technology 2 (UTAUT2), an extended and modified model that encompasses perceived trust was developed. The sample comprised 358 customers from Islamic banks (IBs) in Jordan, and structural equation modelling was applied to examine data drawn from the sample.FindingsThe research framework presented 0.728% of the behavioural intention variance and 0.455% of the use behaviour. Results discovered that performance expectancy, perceived trust and hedonic motivation have significant relations with behavioural intention. The finding that effort expectancy has an insignificant effect and that social influence has a significant negative influence on behavioural intention was unexpected.Research limitations/implicationsThe research has successfully verified the effect of performance expectancy, perceived trust and hedonic motivation on the customer's intention to use IMB smart services. However, the research data findings are based on the cross-sectional design.Practical implicationsThe outcomes hold implications for marketing strategy makers who are responsible for promoting IMB smart services in IBs.Originality/valueThis research presents a deeper insight into IMB adoption and use. The research employed UTAUT and UTAUT2 as the baseline model and incorporates perceived trust to estimate behavioural intention. To the best of the authors' knowledge, this could be the first inquiry that examines IMB smart services adoption and use in Jordan.
Promoting collaborative learning in virtual worlds: the power of “we”
Authors: Li Yang-Jun , Cheung Christy M.K. , Shen Xiao-Liang , Lee Matthew K.O. 
Published: September 2022

PurposeAs digital spaces for team collaboration, virtual worlds bring considerable verisimilitude to technology-mediated social interaction and change the process of traditional team learning. The purpose of this study is to understand how to promote collaborative learning in virtual worlds by leveraging the power of we-intention to participate in virtual worlds. The authors further use the valence–instrumentality–self-efficacy–trust model (VIST) model as a means of understanding the formation of we-intention to participate in virtual worlds, during which behavioral desire serves a bridging role.Design/methodology/approachThe authors tested the research model using the data gathered from 298 users of a prominent form of virtual world, i.e. massively multiplayer online role-playing games. The authors used the structural equation modeling approach and the partial least squares technique for data analysis.FindingsResults show that the four factors of the VIST model (i.e. valence on team goals, instrumentality of contribution, self-efficacy in team tasks and trust in team members) all positively influence we-intention to participate in virtual worlds through behavioral desire for team actions. We-intention to participate in virtual worlds further exerts a stronger positive effect on collaborative learning in virtual worlds, compared with I-intention to participate in virtual worlds.Originality/valueThis work advances the information systems literature by introducing a relevant and important concept, i.e. we-intention, to explain collaborative learning in virtual worlds. This study especially compared the effect of we-intention and I-intention on collaborative learning in virtual worlds. The results of this work also provide practitioners with insights into the role of we-intention in promoting collective actions in virtual worlds.
Women entrepreneurs’ success factors of Northern Indian community: a person–environment fit theory perspective
Authors: Chhabra Meghna , Singh Lata Bajpai , Mehdi Syed Asif 
Published: September 2022

Purpose
Women entrepreneurs contribute significantly to Asian economies. However, women in this region face an alarming array of barriers to entrepreneurship. This research study aims to examine the factors, i.e. government support, family social support, financial literacy and managerial skills, in building the entrepreneurial capacity of women entrepreneurs under the lens of the person–environment (P-E) fit theory. Furthermore, the study also examines the moderating effect of socio-cultural barriers in the said relationships.

Design/methodology/approach
For the study, the data was collected from the owners of 311 women-owned manufacturing and services sector enterprises from the northern Indian community.

Findings
Findings suggest that all the factors significantly affect the entrepreneurial capacity of women entrepreneurs, and the barriers work as a moderator between the relationships.

Originality/value
Based on P-E fit theory, this unique research study proposes a model to test the role of factors such as government support, family social support, financial literacy and managerial skills in developing women entrepreneurs’ entrepreneurial capacity along with examining the moderating role of socio-cultural factors contributing to the entrepreneurial capacity of women.
The primary mental health care nurse specialist practice experience in primary care centers in Saudi Arabia: a qualitative study
Authors: Alhamidi Sami Abdullrahman , Alyousef Seham Mansour 
Published: September 2022

Purpose
This paper aims to explore the value that care from a primary mental health care nurse (PMHCN) can bring to people with mental health (MH) problems in health-care delivery.

Design/methodology/approach
This study used a focus group of 20 PMHCNs working in primary care (PC) health settings in Saudi Arabia before using thematic content analysis to describe and explore their views and experiences of the integration of MH care into PC units. The researchers used a semi-structured interview format, which began with open-ended probes and proceeded to use of theoretical sampling based on emerging data related to their experiences and perceptions in the integration process.

Findings
Four main themes were derived from the thematic analysis of interviews: collaboration, knowledge and experience, awareness and orientation care and influential role.

Research limitations/implications
This study’s use of qualitative research methods has certain limitations, including the small sample size of 20 participants, which means that it may not be representative of all primary MH nurses in primary health-care centers in Saudi Arabia. To make the results applicable to a broader audience, the researcher sought to moderate this limitation by including participants with extensive experience in multiple settings and nurses of different ages.

Practical implications
The cost implications of the PMHCN service are not yet comprehensively understood, but it is apparent that this model is not only regarded extremely positively by MH practitioners but may also have significant benefits in terms of patient outcomes. The configuration of local services and relevant patient demographics will affect the extent to which this study’s findings are transferable. Meanwhile, further research in this area could seek to provide further evidence about the aspects of the PMHCN service model, such as secondary care referrals and waiting times, accident and emergency attendances and patient recovery rates and the impact of providing such a service on health-care practitioners as well as its cost-effectiveness.

Originality/value
This study’s findings suggest that PMHCNs believe that their care improves the quality of PC for patients in PC settings. Elements of the PC placement that professionals particularly valued include their ability to assist patients in their own community and the inclusion of volunteer stakeholders who act as preceptors. The participants expressed a need for improvement of policy related to referrals within the clinics.
Qualitative and quantitative interdependence of mechanical properties of industrially extruded AA6063 alloy on process parameters and profile characteristics
Authors: Abdul Jawwad Abdul Kareem , Al-Bashir Adnan , Saleem Muhammad , Hasanain Bassam 
Published: September 2022

PurposeThis study aims to investigate and model interrelationships between process parameters, geometrical profile characteristics and mechanical properties of industrially extruded aluminum alloys.Design/methodology/approachStatistical design of experiments (DOE) was applied to investigate and model the effects of eight factors including extrusion ratio, stem speed, billet-preheat temperature, number of die cavities, quenching media (water/air), time and temperature of artificial aging treatment and profile nominal thickness on four mechanical properties (yield strength, ultimate tensile strength, percent elongation and hardness). Experiments were carried out at an actual extrusion plant using 8-in. diameter billets on an extrusion press with 2,200 ton capacity.FindingsMain factors and factor interactions controlling mechanical properties were identified and discussed qualitatively. Quantitative models with high prediction accuracy (in excess of 95%) were also obtained and discussed.Practical implicationsThe obtained results are believed to be of great importance to researchers and industrial practitioners in the aluminum extrusion industry.Originality/valueAll practical and relevant parameters have been used to model all important mechanical properties in a collective manner in one study and within actual industrial setup. This is in contrast to all previous studies where either a partial set of parameters and/or mechanical properties are discussed and mostly under limited laboratory setup.