bluuebunny/crossref_metadata_2025_split huggingface dataset:
Paediatric and adult congenital cardiology education and training in Europe
Authors: McMahon Colin J. , Heying Ruth , Budts Werner , Cavigelli-Brunner Anna , Shkolnikova Maria , Michel-Behnke Ina , Kozlik-Feldmann Rainer , Wåhlander Håkan , DeWolf Daniel , Difilippo Sylvie , Kornyei Laslo , Russo Maria Giovanna , Kaneva-Nencheva Anna , Mesihovic-Dinarevic Senka , Vesel Samo , Oskarsson Gylfi , Papadopoulos George , Petropoulos Andreas C. , Cevik Berna Saylan , Jossif Antonis , Doros Gabriela , Krusensjerna-Hafstrom Thomas , Dangel Joanna , Rahkonen Otto , Albert-Brotons Dimpna C. , Alvares Silvia , Brun Henrik , Janousek Jan , Pitkänen-Argillander Olli , Voges Inga , Lubaua Inguna , Sendzikaite Skaiste , Magee Alan G. , Rhodes Mark J. , Blom Nico A. , Bu’Lock Frances , Hanseus Katarina , Milanesi Ornella 
Published: March 2022

AbstractBackground:Limited data exist on training of European paediatric and adult congenital cardiologists.Methods:A structured and approved questionnaire was circulated to national delegates of Association for European Paediatric and Congenital Cardiology in 33 European countries.Results:Delegates from 30 countries (91%) responded. Paediatric cardiology was not recognised as a distinct speciality by the respective ministry of Health in seven countries (23%). Twenty countries (67%) have formally accredited paediatric cardiology training programmes, seven (23%) have substantial informal (not accredited or certified) training, and three (10%) have very limited or no programme. Twenty-two countries have a curriculum. Twelve countries have a national training director. There was one paediatric cardiology centre per 2.66 million population (range 0.87–9.64 million), one cardiac surgical centre per 4.73 million population (range 1.63–10.72 million), and one training centre per 4.29 million population (range 1.63–10.72 million population). The median number of paediatric cardiology fellows per training programme was 4 (range 1–17), and duration of training was 3 years (range 2–5 years). An exit examination in paediatric cardiology was conducted in 16 countries (53%) and certification provided by 20 countries (67%). Paediatric cardiologist number is affected by gross domestic product (R2 = 0.41).Conclusion:Training varies markedly across European countries. Although formal fellowship programmes exist in many countries, several countries have informal training or no training. Only a minority of countries provide both exit examination and certification. Harmonisation of training and standardisation of exit examination and certification could reduce variation in training thereby promoting high-quality care by European congenital cardiologists.
Large CO2 Emitters as Seen From Satellite: Comparison to a Gridded Global Emission Inventory
Authors: Chevallier Frédéric , Broquet Grégoire , Zheng Bo , Ciais Philippe , Eldering Annmarie 
Published: March 2022

AbstractUsing the multiyear archive of the two Orbiting Carbon Observatories (OCO) of NASA, we have retrieved large fossil fuel CO2 emissions (larger than 1.0 ktCO2 h−1 per 10−2 square degree grid cell) over the globe with a simple plume cross‐sectional inversion approach. We have compared our results with a global gridded and hourly inventory. The corresponding OCO emission retrievals explain more than one third of the inventory variance at the corresponding cells and hours. We have binned the data at diverse time scales from the year (with OCO‐2) to the average morning and afternoon (with OCO‐3). We see consistent variations of the median emissions, indicating that the retrieval‐inventory differences (with standard deviations of a few tens of percent) are mostly random and that trends can be calculated robustly in areas of favorable observing conditions, when the future satellite CO2 imagers provide an order of magnitude more data.
Structure of Sea Surface Temperature Anomaly Induced by Mesoscale Eddies in the North Pacific Ocean
Authors: Lv Mingkun , Wang Fan , Li Yuanlong , Zhang Zhengguang , Zhu Yanan 
Published: February 2022

AbstractSea surface temperature anomalies (SSTAs) induced by oceanic mesoscale eddies trigger mesoscale air‐sea interactions and modulate large‐scale climate systems. Yet, how eddies drive SSTAs has not been firmly established; particularly, the relative importance of lateral stirring and vertical pumping remains a debated issue. This study investigates characteristics and generation mechanisms of mesoscale eddy SSTAs in three eddy‐enriched domains of the North Pacific Ocean: the Kuroshio Extension (KET), the Subtropical Countercurrent (STCC), and the North Equatorial Countercurrent (NECC). Analysis of satellite observational data reveals quasi‐monopole eddy SSTAs in the KET and NECC and dipole‐like eddy SSTAs in the STCC. By investigating spatial and seasonal variations and performing sensitivity experiments using an idealized model, we demonstrate that lateral stirring plays a more important role than vertical pumping in causing mesoscale eddy SSTAs. The eastward transport by the strong background current UC (∼0.6 and ∼0.4 m s−1 in the KET and the NECC, respectively) and the westward eddy translation UT (∼−0.2 m s−1 in the NECC) can dramatically modify the structure of SSTA. The pure stirring effect of eddy rotation velocities generates strong dipole‐like SSTAs. Owing to the eastward UC and the westward UT, the western SSTA pole tends to approach the eddy center, while the eastern SSTA pole departs from the eddy and scatters along the eddy trajectory. These effects reduce the eddy SSTA amplitude and favor the emergence of quasi‐monopole structure. This work provides a useful benchmark for model simulations of mesoscale SST variability and air‐sea interaction.
Energetically Informed Niche Models of Hydrogenotrophs Detected in Sediments of Serpentinized Fluids of the Samail Ophiolite of Oman
Authors: Howells Alta E. G. , Leong James A. M. , Ely Tucker , Santana Michelle , Robinson Kirt , Esquivel‐Elizondo Sofia , Cox Alysia , Poret‐Peterson Amisha , Krajmalnik‐Brown Rosa , Shock Everett L. 
Published: February 2022

AbstractA geochemical gradient established by mixing between reduced, hyperalkaline (pH > 11), H2‐rich fluids generated through the process of serpentinization and surrounding surface water (pH ∼ 8) in the Samail Ophiolite of Oman provides an opportunity to characterize the geochemical and biological factors that influence the distribution of H2 oxidizing chemotrophs, hydrogenotrophs. In this study, 16S rRNA gene amplicon sequencing was implemented to characterize hydrogenotrophs in sediments underlying surface expressed serpentinized fluids in Oman. Hydrogenotroph phylotype distribution was evaluated as functions of chemical energy supplies for their given metabolic redox reactions. Through this approach, it was discovered that hydrogenotrophic taxa are likely constrained to sediments with overlying fluids that have <∼60 μm O2, including microorganisms of the genus, Hydrogenophaga. Sulfate reducers of the family, Thermodesulfovibrionaceae, likely require >∼10 μm SO4−2 for survival. In sediments with fluids having >∼10 μm SO4−2, sulfate reducers likely outcompete microorganisms of the methanogen genus, Methanobacterium, for H2. Additionally, differences in distribution between Thermodesulfovibrionaceae and Methanobacterium may be driven by the availability of electron acceptors and the redox reaction that is most energy yielding in the fluid. Taken together, observations from the Oman geochemical gradient result in a hydrogenotroph niche model that can be used to evaluate global distribution patterns of hydrogenotrophs in continental serpentinized fluids. On a global scale, based on previous studies, Methanobacterium is constrained to fluids that have <∼10 μm SO4−2.
Feedforward and feedback interactions between visual cortical areas use different population activity patterns
Authors: Semedo João D. , Jasper Anna I. , Zandvakili Amin , Krishna Aravind , Aschner Amir , Machens Christian K. , Kohn Adam , Yu Byron M. 
Published: March 2022

AbstractBrain function relies on the coordination of activity across multiple, recurrently connected brain areas. For instance, sensory information encoded in early sensory areas is relayed to, and further processed by, higher cortical areas and then fed back. However, the way in which feedforward and feedback signaling interact with one another is incompletely understood. Here we investigate this question by leveraging simultaneous neuronal population recordings in early and midlevel visual areas (V1–V2 and V1–V4). Using a dimensionality reduction approach, we find that population interactions are feedforward-dominated shortly after stimulus onset and feedback-dominated during spontaneous activity. The population activity patterns most correlated across areas were distinct during feedforward- and feedback-dominated periods. These results suggest that feedforward and feedback signaling rely on separate “channels”, which allows feedback signals to not directly affect activity that is fed forward.
Multi-modal profiling of human fetal liver hematopoietic stem cells reveals the molecular signature of engraftment
Authors: Vanuytsel Kim , Villacorta-Martin Carlos , Lindstrom-Vautrin Jonathan , Wang Zhe , Garcia-Beltran Wilfredo F. , Vrbanac Vladimir , Parsons Dylan , Lam Evan C. , Matte Taylor M. , Dowrey Todd W. , Kumar Sara S. , Li Mengze , Wang Feiya , Yeung Anthony K. , Mostoslavsky Gustavo , Dries Ruben , Campbell Joshua D. , Belkina Anna C. , Balazs Alejandro B. , Murphy George J. 
Published: March 2022

AbstractThe human hematopoietic stem cell harbors remarkable regenerative potential that can be harnessed therapeutically. During early development, hematopoietic stem cells in the fetal liver undergo active expansion while simultaneously retaining robust engraftment capacity, yet the underlying molecular program responsible for their efficient engraftment remains unclear. Here, we profile 26,407 fetal liver cells at both the transcriptional and protein level including ~7,000 highly enriched and functional fetal liver hematopoietic stem cells to establish a detailed molecular signature of engraftment potential. Integration of transcript and linked cell surface marker expression reveals a generalizable signature defining functional fetal liver hematopoietic stem cells and allows for the stratification of enrichment strategies with high translational potential. More precisely, our integrated analysis identifies CD201 (endothelial protein C receptor (EPCR), encoded by PROCR) as a marker that can specifically enrich for engraftment potential. This comprehensive, multi-modal profiling of engraftment capacity connects a critical biological function at a key developmental timepoint with its underlying molecular drivers. As such, it serves as a useful resource for the field and forms the basis for further biological exploration of strategies to retain the engraftment potential of hematopoietic stem cells ex vivo or induce this potential during in vitro hematopoietic stem cell generation.
Adaptive translational reprogramming of metabolism limits the response to targeted therapy in BRAFV600 melanoma
Authors: Smith Lorey K. , Parmenter Tiffany , Kleinschmidt Margarete , Kusnadi Eric P. , Kang Jian , Martin Claire A. , Lau Peter , Patel Riyaben , Lorent Julie , Papadopoli David , Trigos Anna , Ward Teresa , Rao Aparna D. , Lelliott Emily J. , Sheppard Karen E. , Goode David , Hicks Rodney J. , Tiganis Tony , Simpson Kaylene J. , Larsson Ola , Blythe Benjamin , Cullinane Carleen , Wickramasinghe Vihandha O. , Pearson Richard B. , McArthur Grant A. 
Published: March 2022

AbstractDespite the success of therapies targeting oncogenes in cancer, clinical outcomes are limited by residual disease that ultimately results in relapse. This residual disease is often characterized by non-genetic adaptive resistance, that in melanoma is characterised by altered metabolism. Here, we examine how targeted therapy reprograms metabolism in BRAF-mutant melanoma cells using a genome-wide RNA interference (RNAi) screen and global gene expression profiling. Using this systematic approach we demonstrate post-transcriptional regulation of metabolism following BRAF inhibition, involving selective mRNA transport and translation. As proof of concept we demonstrate the RNA processing kinase U2AF homology motif kinase 1 (UHMK1) associates with mRNAs encoding metabolism proteins and selectively controls their transport and translation during adaptation to BRAF-targeted therapy. UHMK1 inactivation induces cell death by disrupting therapy induced metabolic reprogramming, and importantly, delays resistance to BRAF and MEK combination therapy in multiple in vivo models. We propose selective mRNA processing and translation by UHMK1 constitutes a mechanism of non-genetic resistance to targeted therapy in melanoma by controlling metabolic plasticity induced by therapy.
Massive interstitial solid solution alloys achieve near-theoretical strength
Authors: Liu Chang , Lu Wenjun , Xia Wenzhen , Du Chaowei , Rao Ziyuan , Best James P. , Brinckmann Steffen , Lu Jian , Gault Baptiste , Dehm Gerhard , Wu Ge , Li Zhiming , Raabe Dierk 
Published: March 2022

AbstractInterstitials, e.g., C, N, and O, are attractive alloying elements as small atoms on interstitial sites create strong lattice distortions and hence substantially strengthen metals. However, brittle ceramics such as oxides and carbides usually form, instead of solid solutions, when the interstitial content exceeds a critical yet low value (e.g., 2 at.%). Here we introduce a class of massive interstitial solid solution (MISS) alloys by using a highly distorted substitutional host lattice, which enables solution of massive amounts of interstitials as an additional principal element class, without forming ceramic phases. For a TiNbZr-O-C-N MISS model system, the content of interstitial O reaches 12 at.%, with no oxides formed. The alloy reveals an ultrahigh compressive yield strength of 4.2 GPa, approaching the theoretical limit, and large deformability (65% strain) at ambient temperature, without localized shear deformation. The MISS concept thus offers a new avenue in the development of metallic materials with excellent mechanical properties.
Aragonite dissolution protects calcite at the seafloor
Authors: Sulpis Olivier , Agrawal Priyanka , Wolthers Mariette , Munhoven Guy , Walker Matthew , Middelburg Jack J. 
Published: March 2022

AbstractIn the open ocean, calcium carbonates are mainly found in two mineral forms. Calcite, the least soluble, is widespread at the seafloor, while aragonite, the more soluble, is rarely preserved in marine sediments. Despite its greater solubility, research has shown that aragonite, whose contribution to global pelagic calcification could be at par with that of calcite, is able to reach the deep-ocean. If large quantities of aragonite settle and dissolve at the seafloor, this represents a large source of alkalinity that buffers the deep ocean and favours the preservation of less soluble calcite, acting as a deep-sea, carbonate version of galvanization. Here, we investigate the role of aragonite dissolution on the early diagenesis of calcite-rich sediments using a novel 3D, micrometric-scale reactive-transport model combined with 3D, X-ray tomography structures of natural aragonite and calcite shells. Results highlight the important role of diffusive transport in benthic calcium carbonate dissolution, in agreement with recent work. We show that, locally, aragonite fluxes to the seafloor could be sufficient to suppress calcite dissolution in the top layer of the seabed, possibly causing calcite recrystallization. As aragonite producers are particularly vulnerable to ocean acidification, the proposed galvanizing effect of aragonite could be weakened in the future, and calcite dissolution at the sediment-water interface will have to cover a greater share of CO2 neutralization.
Subnanometric alkaline-earth oxide clusters for sustainable nitrate to ammonia photosynthesis
Authors: Li Jieyuan , Chen Ruimin , Wang Jielin , Zhou Ying , Yang Guidong , Dong Fan 
Published: March 2022

AbstractThe limitation of inert N2 molecules with their high dissociation energy has ignited research interests in probing other nitrogen-containing species for ammonia synthesis. Nitrate ions, as an alternative feedstock with high solubility and proton affinity, can be facilely dissociated for sustainable ammonia production. Here we report a nitrate to ammonia photosynthesis route (NO3−RR) catalyzed by subnanometric alkaline-earth oxide clusters. The catalyst exhibits a high ammonia photosynthesis rate of 11.97 mol gmetal−1 h−1 (89.79 mmol gcat−1 h−1) with nearly 100% selectivity. A total ammonia yield of 0.78 mmol within 72 h is achieved, which exhibits a significant advantage in the area of photocatalytic NO3−RR. The investigation of the molecular-level reaction mechanism reveals that the unique active interface between the subnanometric clusters and TiO2 substrate is beneficial for the nitrate activation and dissociation, contributing to efficient and selective nitrate reduction for ammonia production with low energy input. The practical application of NO3−RR route in simulated wastewater is developed, which demonstrates great potential for its industrial application. These findings are of general knowledge for the functional development of clusters-based catalysts and could open up a path in the exploitation of advanced ammonia synthesis routes with low energy consumption and carbon emission.
Tree functional traits, forest biomass, and tree species diversity interact with site properties to drive forest soil carbon
Authors: Augusto Laurent , Boča Antra 
Published: March 2022

AbstractForests constitute important ecosystems in the global carbon cycle. However, how trees and environmental conditions interact to determine the amount of organic carbon stored in forest soils is a hotly debated subject. In particular, how tree species influence soil organic carbon (SOC) remains unclear. Based on a global compilation of data, we show that functional traits of trees and forest standing biomass explain half of the local variability in forest SOC. The effects of functional traits on SOC depended on the climatic and soil conditions with the strongest effect observed under boreal climate and on acidic, poor, coarse-textured soils. Mixing tree species in forests also favours the storage of SOC, provided that a biomass over-yielding occurs in mixed forests. We propose that the forest carbon sink can be optimised by (i) increasing standing biomass, (ii) increasing forest species richness, and (iii) choosing forest composition based on tree functional traits according to the local conditions.
Prenatal and early life exposure to air pollution and the incidence of Kawasaki disease
Authors: Kuo Ni-Chun , Lin Chien-Heng , Lin Ming-Chih 
Published: March 2022

AbstractKawasaki disease (KD) is the most common form of acquired pediatric cardiac disease in the developed world. However, its etiology is still unclear. Epidemiological studies have shown that air pollution is a plausible risk factor in stimulating oxidative stress, inducing inflammation and causing autoimmune diseases. This study aims to assess the connections between prenatal and early life air pollution exposure to the incidence of KD. The main data source of this nationwide longitudinal study was the National Health Insurance Research Database (NHIRD) of Taiwan. NHIRD was linked with Taiwan Maternal and Child Health Database to establish the link between mothers and children. In total, 4192 KD cases involving children under 6 years of age were identified between January 2004 and December 2010. Children in the control group were randomly selected at a 1:4 ratio and matched using their age and index year. Integrated data for the air pollutants were obtained from 71 Environmental Protection Agency monitoring stations across Taiwan. Patients who had main admission diagnosis of KD and subsequently received intravenous immunoglobulin treatment were defined as incidence cases. Ambient exposure, including pollutant standards index (PSI), carbon monoxide (CO), nitric oxide (NO), nitric dioxide (NO2), and nitrogen oxide (NOx) during pregnancy were all positively associated with KD incidence. Conversely, ozone (O3) exposure had a negative correlation. Exposure to CO, NO, NO2, and NOx after childbirth remained consistent with regards to having a positive association with KD incidence. Exposure to PSI and O3 after delivery displayed no significant association with KD. Both prenatal and postnatal cumulative CO, NO, NO2, and NOx exposure had a dose dependent effect towards increasing KD incidence. Certain prenatal and early life air pollutant exposure may increase the incidence of KD.
Transposase-CRISPR mediated targeted integration (TransCRISTI) in the human genome
Authors: Rezazade Bazaz Mahere , Ghahramani Seno Mohammad M. , Dehghani Hesam 
Published: March 2022

AbstractVarious methods have been used in targeted gene knock-in applications. CRISPR-based knock-in strategies based on homology-independent repair pathways such as CRISPR HITI have been shown to possess the best efficiency for gene knock-in in mammalian cells. However, these methods suffer from the probability of plasmid backbone insertion at the target site. On the other hand, studies trying to combine the targeting ability of the Cas9 molecule and the excision/integration capacity of the PB transposase have shown random integrations. In this study, we introduce a new homology-independent knock-in strategy, Transposase-CRISPR mediated Targeted Integration (TransCRISTI), that exploits a fusion of Cas9 nuclease and a double mutant piggyBac transposase. In isogenic mammalian cell lines, we show that the TransCRISTI method demonstrates higher efficiency (72%) for site-specific insertions than the CRISPR HITI (44%) strategy. Application of the TransCRISTI method resulted in site-directed integration in 4.13% and 3.69% of the initially transfected population in the human AAVS1and PML loci, respectively, while the CRISPR HITI strategy resulted in site-directed integration in the PML locus in only 0.6% of cells. We also observed lower off-target and random insertions in the TransCRISTI group than the CRISPR HITI group. The TransCRISTI technology represents a great potential for the accurate and high-efficiency knock-in of the desired transposable elements into the predetermined genomic locations.
Association between chronic kidney disease and open-angle glaucoma in South Korea: a 12-year nationwide retrospective cohort study
Authors: Ro Jun-Soo , Moon Jong Youn , Park Tae Kwann , Lee Si Hyung 
Published: March 2022

AbstractVarious non-intraocular pressure factors have been identified as possible risk factors for open-angle glaucoma (OAG). However, there is still controversy around the association between OAG and chronic kidney disease (CKD). In this study, we used a nationwide cohort to investigate the risk of OAG in the 12 years following a diagnosis of CKD. This retrospective cohort study included 1,103,302 subjects from the Korean National Health Insurance Service National Sample Cohort database. The CKD group (n = 1318) included patients who were initially diagnosed with CKD between 2003 and 2008. The subjects in the comparison group were matched at a 1:5 ratio using propensity scores. In multivariate Cox regression analysis, a diagnosis of CKD was significantly associated with an increased incidence of OAG (hazard ratio [HR] = 1.546, 95% confidence interval [CI] 1.363–1.754, p < 0.001). Further analysis revealed that the risk of OAG increased with the severity of CKD (mild to moderate CKD [CKD stage 1–3]: HR = 1.280, 95% CI 1.077–1.521, p = 0.005; advanced CKD [CKD stage 4–5]: HR = 1.861, 95% CI 1.589–2.180, p < 0.001). In subgroup analysis, female CKD patients had a greater risk of developing OAG than males, and subjects with CKD aged ≥ 40 years were more likely to develop OAG compared with those aged < 40 years. Our study demonstrates that CKD is a significant risk factor for OAG and that severe CKD is associated with an increased risk of developing OAG.