bluuebunny/crossref_metadata_2025_split huggingface dataset:
A dihydropyridine derivative as a highly selective fluorometric probe for quantification of Au3+ residue in gold nanoparticle solution
Authors: Paisuwan Waroton , Sukwattanasinitt Mongkol , Tobisu Mamoru , Ajavakom Anawat 
Published: October 2022

Abstract
Novel dihydroquinoline derivatives (DHP and DHP-OH) were synthesized in one pot via a tandem trimerization-cyclization of methylpropiolate. DHP and DHP-OH possess strong blue fluorescence with high quantum efficiencies over 0.70 in aqueous media. DHP-OH displays a remarkable fluorescence quenching selectively to the presence of Au3+ through the oxidation of dihydropyridine to pyridinium ion as confirmed by NMR and HRMS. DHP-OH was used to demonstrate the quantitative analysis of Au3+ in water samples with limit of detection of 32.8 ppb and excellent recovery (>95%). This fluorescent probe was also applied for determination of Au3+ residue in the gold nanoparticle solution and paper-based sensing strip for on-site detection of Au3+.
Medical image enhancement based on volumetric tissue segmentation fusion (Uni-stable 3D method)
Authors: ELAFF Ihab 
Published: October 2022

Abstract
The 3D Uni-stable method is a novel technique for 3D medical image enhancement which produces 3D Images of high contrast from the scanned anisotropic scaling images. This is done by estimating some intermediate slices through resizing the original scans. Rescaling has been achieved at three different levels: rescaling of eigenvalues of diffusion, rescaling the Scalar Indexes from the original eigenvalues, and rescaling the cluster maps of the segmentation of the original Scalar Indexes. Four interpolation methods have been employed at each level and four clustering algorithms have been employed in the process. The 3D Uni-stable image is almost universal as it combines variety of algorithms points of views into one 3D probability map. This reduces boundary-overlapping among different tissues, and hence improves the uniqueness of the segmentation problem solution. The stability factor of the 3D Uni-stable-Images is measured by maximum match analysis between the cluster maps which are generated from 3D Uni-stable images using variety of clustering methods with respect to true fact references for 5 different brains and the resultant standard deviations of Uni-stable images maximum match analysis in both threshold and tissue to brain ratio are much lower than Mean Diffusivity and Fractional Anisotropy scalar indexes for both CSF/non-CSF and WM/non-WM respectively.
10-year experience with staggered multimodal treatment in brain arteriovenous malformations in a single medical center in Mexico. An Observational Cohort Study
Authors: Melo-Guzmán Gustavo , Nicolás-Cruz Carlos Fernando , Montesinos-Sampedro Alberto , Mendizabal-Guerra Rafael , Santiago-Germán David 
Published: October 2022

Abstract
An observational cohort study was performed in patients with diagnosis of brain AVM in a third level medical center in Mexico City, from December 2007 to January 2018. Patients were divided into 3 groups: small (S-M Grade I and II), Transitional (S-M Grade III) and Giant (S-M Grade IV and V). and then these groups subcategorized in bimodal treatment (embolization plus microsurgery) and others (microsurgery, radiosurgery, conservative). Functional outcome was assessed with the modified Rankin Scale (mRS) and compared between the 2 groups before treatment and 24 months follow-up.
97 patients were diagnosed, 22 excluded due to inconsistency in medical records or abandoning the treatment. The final sample analyzed was 75 patients. The overall had a mean age of 22 [14-38] years and the predominant sex was male in 55% (n=41). The clinical presentation was: focal neurological deficit 28% (n=21), seizures 27% (n=20), headache 24% (n=18), and intracranial hemorrhage 21% (n=16). Only seven (9.3%) patients from the total sample developed trans-procedural rupture of brain AVM, After 24 months follow up 24 (31.9%) patients remains with neurological sequel, and four (5.3%) died. In low grade brain AVMs the mean MRS decreased from 2.5±1.2 to 0.7±0.9, in transitional grade brain AVMs from 2.3±1.7 to 0.9±1, and in giant AVMs 2.4±1.1 to 2.3±2.1.
Bimodal or multimodal treatment should be carefully considered in high-grade arteriovenous malformations. The main objective, having embolization as a first step is to reduce the risk of intraoperative bleeding, obliterating feeder arteries that will be difficult to control with microsurgery. Additionally, the risk of trans procedure rupture of the bAVM is directly proportional to the “aggressiveness” which it is treated.
A Cost-effectiveness Analysis of Adding Cetuximab to the First-line Treatment of Metastatic Colorectal Carcinoma in Iran; Considering Genetic Screening for Precision Medicine
Authors: Yousefi Nazila , Salimi Alireza , Mohammadnezhad Ghader , Taheri Saeed , Peiravian Farzad 
Published: October 2022

Abstract
Purpose

This study aimed to evaluate the cost-effectiveness of cetuximab in different genetic populations of metastatic colorectal carcinoma patients, including KRAS and RAS wild types and mutants, when added to FOLFIRI treatment regimens for evidence-based disease management in Iran.

Method

A Markov decision model was designed in TreeAge software with the three states of stable, progress, and death. Clinical outcomes were extracted from published clinical studies, and costs were extracted from the Iranian local data. The primary outcome was an incremental cost-effectiveness ratio (ICER) in the simulated population.

Results

The cost-utility model from the perspective of the health system indicated that the average direct medical costs of a patient that has not been genetically screened are $56985.27 and $20767.74 in FOLFIRI + cetuximab and FOLFIRI regimens, respectively. However, costs per patient in the KRAS wild-type population were $21845.52 in FOLFIRI and $78,321.22 in FOLFIRI + cetuximab. In RAS wild-type patients, FOLFIRI and FOLFIRI + cetuximab costs per patient were $23111.62 and $84976.39, respectively. Incremental QALYs for the above scenarios were 0.069, 0.193, and 0.285, respectively. Therefore, the ICER of add-on cetuximab in Iran compared to the treatment alternatives in the scenarios with and without KRAS screening was $520771.55/QALY, $292768.16/QALY, and $217460.51/QALY.

Conclusion

Although genetic screening in precision medicine reduces costs per outcome, according to the willingness-to-pay threshold of $4349.50 in the Iranian health system, add-on cetuximab to the FOLFIRI regimen is not a cost-effective strategy even with genetic screening and a 20% price reduction.
Combining individual tracking data with high-resolution thermal mapping to identify microclimate refugia for a threatened grassland bird
Authors: Ramos Rita F. , Franco Aldina M. A. , Gilroy James J. , Silva João P. 
Published: October 2022

Abstract

Context
Local microclimate refugia may buffer against detrimental effects of extreme temperatures preventing fitness loss.
Objectives
By combining high resolution habitat and climate information with GPS tracking data, we develop a novel approach to identify the use and availability of cooler microclimate refugia within large landscapes.
Methods
GPS data from 77 little bustards (Tetrax tetrax), an endangered grassland bird tracked between 2009 and 2019, was combined with hourly temperature information at 30x30m resolution. 92,685 GPS locations and their surrounding 500m areas were characterised with temperature and habitat information and used to determine the availability and use of microclimate refugia (sites > 0.5˚C cooler than the surrounding landscape).
Results
We found that the semi-natural grassland landscapes used by little bustards have limited availability of cooler microclimate areas – fewer than 30% of the locations provided microclimate refugia opportunities. The use of cooler microclimate sites by little bustards increased at higher ambient temperatures, suggesting that individuals actively utilise microclimate refugia to cope with extreme heat conditions. Microclimate refugia availability and use were greater in areas with heterogeneous vegetation cover, and in coastal areas.
Conclusions
This study identified the landscape characteristics that provide microclimate opportunities and shelter from extreme heat conditions. Little bustards made greater use of microclimate refugia with increasing temperatures, particularly during the breeding season when individuals are highly site faithful. This information can help identify areas where populations might be particularly exposed to climate extremes due to a lack of refugia and where habitat management measures should take place.
Insights into genetic differentiation and migration routes of rhesus macaques (Macaca mulatta) based on samples from almost all distribution areas
Authors: Zhang Lin , Yao Yongfang , Su Qian , Zhao Junsong , Zhou Fei , Yu Mengshi , Xiao Hongtao , Xie Meng , Xiong Ying , Wu Jiayun , Ni Qingyong , Zhang Mingwang , Xu Huai-liang 
Published: October 2022

AbstractBackground Rhesus macaque (Macaca mulatta) is commonly used in biomedical research because of its high similarity with human beings, and understand the genetic diversity of wild macaques is necessary. Although there have been many genetic diversity reports on this species, there is still a deficiency of comprehensive understanding of genetic background due to limited sample range, especially, involving Qinghai-Tibet Plateau samples. Here, extensive sampling is conducted in macaque populations across almost all distribution areas, including China, India, Vietnam, Bangladesh, Nepal and Myanmar. It also focuses on genetic differentiation, migration diffusion and population dynamics in western China. Results 57 unique haplotypes were obtained from 452 samples in 34 geographical areas and 60 mtDNA haplotypes were downloaded, resulting in a total of 117 haplotypes covering almost all macaque distribution areas. The phylogenetic analysis exhibited three major haplogroups (Clade Ⅰ~Ⅲ) with obvious geographical distribution patterns. Clade Ⅰ is mainly composed of macaques from western China, Clade Ⅱ is from central and eastern China, Vietnam, India and Nepal, and Clade Ⅲ is from Bangladesh and Myanmar. The divergence age among three Clade is 1.67 ~ 2.81 Ma. Further, Clade Ⅰ from western China is divided into five obviously divergent subclades (Sub A-E). We found macaques in western Sichuan contain two morphology subspecies:M. m. lasiotusandM. m. vestita, different from the previous one subspecies (M. m. lasiotus) report. Notably, comprehensive analysis suggested that the populations in southeastern Sichuan, eastern Yunnan and Guizhou (Sub B) may be an independent new subspecies. We also found Bangladesh may be a re-radiation central of the whole rhesus macaques, and three possible radiation routes of rhesus macaques entering China were speculated: from Yunnan to western China, from Guangxi to central and eastern China, and along the coastline to Hainan. In addition, demographic history analysis revealed the macaque population is stable and has not experienced significant population expansion. Conclusions In conclusion, this study investigated the distribution patterns of different subspecies of macaques, and proposed possible radiation routes. To our knowledge, it’s the first comprehending analysis based on almost all distribution areas of rhesus macaques.
Immunoinfiltration-related gene SERPINE1 is a novel biomarker for diagnosis and prognosis of stomach adenocarcinoma discovered via high throughput transcriptome data mining combined with bioinformatics
Authors: Zhai Yiyan , Liu Xinkui , Huang Zhihong , Zhang Jingyuan , Stalin Antony , Tan Yingying , Zhang Fanqin , Chen Meilin , Shi Rui , Huang Jiaqi , Wu Chao , Wu Zhishan , Lu Shan , You Leiming , Wu Jiarui 
Published: October 2022

Abstract
Stomach adenocarcinoma (STAD) is a type of cancer as it has frequently developed into the advanced stage at diagnosis and mortality. Several factors influence the prognosis of STAD, including the expression and regulation of immune cells in the tumor microenvironment (TME). In this study, we aim to find biomarkers related to the diagnosis and prognosis of gastric cancer, hoping to provide new ideas for the diagnosis and treatment of gastric cancer in the future. STAD and normal patient RNA sequencing data sets are accessed from The Cancer Genome Atlas (TCGA database). Differential genes are determined and obtained using the R package DESeq2. The stromal, immune, and ESTIMATE scores are calculated using the algorithm ESTIMATE, and based on this, the R package WGCNA is used to screen modular genes. Subsequently, the intersection between the modular gene and the differential gene was taken and the STRING database was used for PPI network module analysis. The R packages clusterProfiler, enrichplot, and ggplot2 were used for GO and KEGG enrichment analysis. Cox regression analysis was used to screen survival-related genes, and finally, the R package Venn Diagram was used to take the intersection and obtain 7 hub genes. The time-dependent ROC curve and Kaplan-Meier survival curve were used to find the SERPINE1gene, which plays a key role in prognosis. Finally, the expression pattern, clinical characteristics, and regulatory mechanism of SERPINE1 were analyzed in STAD. This study revealed that the expression of SERPINE1 was significantly increased in the samples from STAD compared with normal samples. Cox regression analysis, time-dependent ROC curve and Kaplan-Meier survival curve showed that SERPINE1 was significantly related to the adverse prognosis of STAD patients. The expression of SERPINE1 increased with the progression of T, N, and M classification of the tumor. In addition, the results of immune infiltration analysis showed that the expression of CD4+ T cells, B cells, CD8+ T cells, macrophages, neutrophils and other immune cells were higher in the group with high SERPINE1 expression than in the group with low SERPINE1 expression. SERPINE1 was closely related to immune cells in the STAD immune microenvironment and had a synergistic effect with the immune checkpoints PD1 and PD-L1. In conclusion, this study proves that SERPINE1 can be used as a prognostic and diagnostic biomarker for STAD and a potential target for immunotherapy.
Hormonal and molecular characterization of calcium oxalate stone formers predicting occurrence and recurrence
Authors: Elshal Ahmed M. , Shamshoun Heba , Awadalla Amira , Elbaz Ramy , Ahmed Asmaa E. , El-khawaga Omali Y. , Shokeir Ahmed A. 
Published: October 2022

Abstract
Abstract
Purpose:
To investigate the role of sex hormones, androgen receptors (ARs) and mi-RNA/CSF-1 in occurrence and recurrence of calcium oxalate (CaOx) renal urolithiasis.
Materials and Methods:
In this prospective study, 74 patients with CaOx stones; stone formers group (SFG) and 40 healthy subjects; control group were compared. SFG includes both denovo and recurrent cases.
Steroid sex hormone plasma assay including testosterone, free testosterone, dihydrotestosterone, estradiol, and sex hormone binding globulin were analyzed. ARs, mi-RNA 185-5p and CSF-1 expression were compared between groups.
Results:
SFG showed significant higher ARs and mi-RNA 185-5p expression (3.7 ± 1.3, 1.8 ± 0.4, respectively) than control group (1 ± 0.08 and 1± 0.07, respectively) (p < 0.05).
However, CSF-1 expression was significantly lower in stone formers than control group. (0.4 ± 0.19 vs 1 ± 0.1, respectively) (p < 0.05). No differences were detected between denovo and recurrent SFG regarding sex hormones, AR, mi-RNA or CSF-1 expression.
Conclusion:
Our data suggest the important role of AR. mi-RNA and CSF-1 signaling
in human nephrolithiasis pathogenesis.
Significance of HLA in the development of Graves’ orbitopathy
Authors: Stasiak Magdalena , Zawadzka-Starczewska Katarzyna , Tymoniuk Bogusław , Stasiak Bartłomiej , Lewiński Andrzej 
Published: October 2022

Abstract
Graves’ disease (GD), similarly to most autoimmune disease, is triggered by environmental factors in genetically predisposed individuals. Particular HLA alleles increase or decrease GD risk. No such correlation was demonstrated for Graves’ orbitopathy (GO) in Caucasian population. HLA-A, -B, -C, -DQB1 and -DRB1 genotyping was performed using a high-resolution method in a total number of 2378 persons including 70 patients with GO, 91 patients with non-GO GD and 2217 healthy controls to compare allele frequencies between GO, non-GO and controls. Significant associations between GO and HLA profile were demonstrated, with HLA-A*01:01, -A*32:01, -B*37:01, -B*39:01, -B*42:01, -C*08:02, C*03:02, DRB1*03:01, DRB1*14:01 and DQB1*02:01 being genetic markers of increased risk of GO, and HLA-C*04:01, -C*03:04, -C*07:02 and DRB1*15:02 being protective alleles. Moreover, correlations between HLA alleles and increased or decreased risk of non-GO GD, but with no impact on risk of GO development, were revealed. Identification of these groups of GO-related and GO-protective alleles, as well as the alleles strongly related to non-GO GD, constitutes an important step in a development of personalized medicine, with individual risk assessment and patient-tailored treatment.
Health-related Quality-of-life outcomes following Roux-en-Y-Gastric-Bypass versus One-Anastomosis- Gastric-Bypass
Authors: Rheinwalt Karl Peter , Fobbe Anna , Plamper Andreas , Alizai Patrick Hamid , Schmitz Sophia Marie-Therese , Brol Maximilian Joseph , Trebicka Jonel , Neumann Ulf Peter , Ulmer Tom Florian 
Published: October 2022

Abstract
Purpose Roux-en-Y-Gastric-Bypass (RYGB) and One-Anastomosis-Gastric-Bypass (OAGB) are effective standard bariatric surgeries with comparable weight loss and remission of obesity-related comorbidities. As procedure-specific health-related quality-of-life (HrQoL) outcomes have not been directly compared thus far, we conducted this questionnaire-based study.
Methods 240 patients after undergoing either RYGB or OAGB between 2011 and 2016 were contacted and asked to fill out SF36 and BAROS questionnaires. All statistical analysis was performed with Microsoft Excel and GraphPad Prism. Primary objectives were procedure-dependent differences in HrQoL. Secondary objectives were weight loss and remission of comorbidities.
Results 119 of 240 contacted patients (49.6%) replied, 58 after RYGB (48.7%) and 61 after OAGB (51,3 %). Follow-up period was <24 months in 52 and >24 months in 64 evaluable patients. Mean age was 46 years (range 23 to 71).
Regarding the <24 months groups, both physical and psychological SF36 sum scales were comparably high. Only the subcategory “general health perception“ was significantly better after RYGB. Significantly higher excess weight loss (EWL) after RYGB (88.81%) compared to OAGB (66.25%) caused significantly better global <24 months BAROS outcomes, whereas remission of comorbidities and HrQoL were similar. Both >24 months groups showed high SF36-HrQoL sum scales. Global mean BAROS results after >24 months were “very good“ in both procedures. EWL in RYGB (80.81%) and in OAGB (81.36%) were comparably excellent.
Conclusion Concerning SF36 and BAROS evaluated HrQoL in early and late postoperative phases, both procedures demonstrated comparable and relevant improvements. Further (preferably randomized) studies should include evaluation of preoperative HrQoL.
Predicting failed access in unstented ureteroscopy
Authors: Morgan Kevin , Possoit HarLee , Connelly Zachary , Frilot Clifton , Khater Nazih , Gomelsky Alexander 
Published: October 2022

Abstract
Introduction/Objectives: The risk of failed access (FA) in unstented ureteroscopy ranges from 7.7%-16%, with young females and those with prior ipsilateral stone surgery, narrow ureteral anatomy, and proximal ureteral stone location carrying higher risk. We aim to determine the rate of failed access at our institution and analyze demographic, clinical, and operative variables associated with FA.
Methods/Materials: We conducted a review of all unstented ureteroscopy procedures at our institution between January 2018 and June 2022. Ureteroscopy for stone, stricture, and neoplasm were included. The primary endpoint was rate of FA, when the unstented ureter failed to accommodate the ureteroscope distal to the target lesion. Demographic, clinical, and operative variables were analyzed to determine if there was an association with FA.
Results: Of the 562 ureteroscopies cases reviewed, 221 unstented ureteroscopies fit our inclusion criteria. FA occurred in 34 (15.4%). Previous stone passage (p=0.039) and distal ureteral location(p=0.042) were associated with successful access (SA). Proximal ureteral location was associated with FA (p=0.008). There was no association with other demographic, clinical and operative variables.
Conclusions: FA occurred at a rate of 15.4% at our institution. Previous stone passage and distal ureteral location were associated with SA, while proximal ureteral location was associated with FA. Prospective studies are needed to better determine predictors of FA.
Epilepsy Treatment Outcome, Adherence to Anti-seizure Medications and Predicting factors at the chronic care facility in Jimma Universtiy Medical Center, Jimma, Southwest Ethiopia: Cross-sectional study
Authors: Shuma Firafan , Terefe Behailu , Tekassa Tamirat 
Published: October 2022

Abstract

The aim of this study was to assess epilepsy treatment outcome, adherence to Anti-seizure medication (ASM), and its predictors among epileptic patients on follow-up at the chronic care unit of Jimma University Medical Center with a total of 168 epileptic patients enrolled in the study. Data was collected by data collectors using data abstraction formats, then entered and analyzed using SPSS version 26.0. Multiple logistic regression analysis was performed to identify the association between dependent and independent variable. In this study, 132(78.6%) patients were adherent to their ASMs. Seizure recurrence was identified in 120(71.4%) patients. Rural residence (AOR = 6.42, 95% CI: 1.32, 31.28, P-value = 0.02), chronic ASM therapy for above five years (AOR = 20.86, 95% CI: 2.66, 163.77, P-value = 0.00), and complaint of ASM-associated undesirable effect (AOR = 13.51, 95% CI: 2.72, 67.26, P-value = 0.00) significantly increased the probability of seizure recurrence. On the other hand, the presence of seizure triggering factor(s) (AOR = 0.12, 95% CI: 0.02, 0.64, P-value = 0.01) decreased the probability of seizure recurrence by 88%.