bluuebunny/crossref_metadata_2025_split huggingface dataset:
Amoebic gill disease (AGD) in Atlantic salmon investigated through a holo-omic lens
Authors: Thormar Eiríkur Andri , Fraslin Clémence , Limborg Morten T. , Robledo Diego 
Published: February 2025

ABSTRACTInteractions between host genetics and the resident microbiota are complex. Understanding these interactions offers interesting alternatives for addressing gill health and disease resistance in salmonids. Amoebic gill disease (AGD), caused byNeoparamoeba perurans,remains a threat to Atlantic salmon, particularly in aquaculture settings where prevention and treatment options are scant. Selective breeding or genetic engineering towards increased AGD resilience present viable prevention strategies. While a number of studies have addressed AGD resistance in Atlantic salmon using transcriptomic and quantitative genetic approaches, the role of the Atlantic salmon gill microbiota in influencing AGD resilience and susceptibility needs further investigation. Addressing this, we leveraged a holo-omic approach using 16S rRNA bacterial profiling, and quantitative genetics, by considering the microbiota as an extended resistance trait of the salmon. We investigated the microbiota of AGD challenged Atlantic salmon in terms of two common resistance indicator traits: gill score and amoebic load. Subsequently we performed a GWAS using both the traditional indicator traits and traits of the microbiota. We found that the gill microbiota of the AGD affected salmon in this study was dominated by two bacterial familiesSimkaniaceaeandArcobacteracea. We found that microbial diversity and the relative abundance ofSimkaniaceae,potentially derived from the amoeba, varied moderately with indicator traits. Additionally we identified several genomic regions that showed suggestive association with gill score and traits of the microbiota, and explored potential candidate genes for AGD resistance. Although much is still unclear regarding gill microbiota dynamics in gill disease, this study highlights the potential of addressing AGD through an integrative approach that considers the interplay between host genetics, the microbiota, and their roles in disease resistance.
Measurement Quality Metrics to Improve Absolute Microbial Cell Counting
Authors: Parratt K. , Newton D. , Dunkers J. , Dootz J. , Hunter M. , Logan-Jackson A. , Pierce L. , Sarkar S. , Servetas S. , Lin NJ. 
Published: February 2025

AbstractTotal and viable microbial cell counts are increasingly important for applications including live biotherapeutic products, food safety, and probiotics. In microbiology, cells are quantified using methods such as colony forming unit (CFU), flow cytometry, and polymerase chain reaction (PCR), but different methods measure different aspects of the cells (measurands), and results may not be directly comparable across methods. In the absence of a ground-truth reference material for cell count, one cannot quantify the accuracy of any cell counting method, which limits method performance assessments and comparisons. Herein, a modified analysis of cell counting methods based on the ISO 20391-2:2019 standard was developed and demonstrated for microbial cell samples diluted over a log-scale range of concentrations.Escherichia colisamples ranging in concentration from approximately 5 × 105cells/mL to 2 × 107cells/mL were quantified using CFU, Coulter principle, fluorescence flow cytometry, and impedance flow cytometry. Quality metrics modified from the ISO standard were calculated for each method and shown to be repeatable across replicate experiments. The quality metrics illustrate large differences in proportionality and variability across methods, with total cell counts in good agreement and viable cell count having more variability. As the ISO standard is meant to guide fit-for-purpose method selection, interpretation of the results and quality metrics can drive method choice and optimization. The framework introduced here will help researchers select fit-for-purpose counting methods for quantification of microbial total and viable cells across a range of applications.
Human newborns form musical predictions based on rhythmic but not melodic structure
Authors: Bianco R. , Tóth B. , Bigand F. , Nguyen T. , Sziller I. , Háden G.P. , Winkler I. , Novembre G. 
Published: February 2025

AbstractThe ability to anticipate rhythmic and melodic structures in music is considered a fundamental human trait, present across all cultures and predating linguistic comprehension in human development. Yet, it remains unclear the extent to which this ability is already developed at birth.Here, we used temporal response functions to assess rhythmic and melodic neural encoding in newborns (N = 49) exposed to classical monophonic musical pieces (real condition) and control stimuli with shuffled tones and inter-onset intervals (shuffled condition). We computationally quantified context-based rhythmic and melodic expectations and dissociated these high-level processes from low-level acoustic tracking, such as local changes in timing and pitch.We observed encoding of probabilisticrhythmicexpectations only in response to real but not shuffled music. This proves newborns’ ability to rely on rhythmic statistical regularities to generate musical expectations. We found no evidence for the tracking ofmelodicinformation demonstrating a downweighing of this dimension compared to the rhythmic one.This study provides neurophysiological evidence that the capacity to track statistical regularities in music is present at birth and driven by rhythm. Melodic tracking, in contrast, may receive more weight through development with exposure to signals relevant to communication, such as speech and music.SignificancePerception and appreciation of music stem from humans’ universal ability to track sequential pitch and timing variations, forming musical melodies and rhythms. To investigate the neurobiological origins of this capacity, we measured neural encoding of rhythm and melody in newborns listening to naturalistic music. We demonstrate a precocious ability to track high-level rhythmic statistical regularities beyond low-level acoustic features. Conversely, melodic tracking is virtually absent at birth, likely emerging with later exposure to pitch-varying signals such as speech and music. This reveals that newborns gradually rely on different sound dimensions to start making sense of the auditory environment. While rhythm and melody are both universal components of music, they do not emerge in parallel but instead develop along distinct timelines.
Repairing subsequent entrepreneurship legitimacy for new ventures: impression management of entrepreneurial-failure stigma
Authors: Xiong Zhuang , Yang HuiMin , Zhang Chenyi , Yang Shiqi , Wang Pengju 
Published: February 2025

PurposeAddressing the adverse effects of entrepreneurial failure stigma and establishing the subsequent entrepreneurship legitimacy of new ventures are crucial for reinvigorating entrepreneurial endeavors. This paper investigated the implementation of impression-management strategies for mitigating the adverse effects of the stigma associated with entrepreneurial failure on the legitimacy of subsequent entrepreneurial activities.Design/methodology/approachDrawing on impression-management theory and legitimacy theory, we conducted three experimental studies to explore the impact of impression-management strategies of entrepreneurial-failure stigma on the legitimacy of subsequent entrepreneurship. Additionally, the role of stakeholders’ forgiveness as a mediating factor and how the specific type of entrepreneurial failure stigma (attributable vs non-attributable) moderates these effects were analyzed.FindingsThe results from Studies 1 and 2 show that implementing impression-management strategies positively contributes to sustaining the legitimacy of subsequent entrepreneurial endeavors in failed new ventures. Assertive strategies demonstrate a more effective impact on enhancing the legitimacy of subsequent entrepreneurship than defensive impression management strategies. In addition, stakeholders’ forgiveness plays a mediating role in the relationship between impression-management strategies and subsequent entrepreneurship legitimacy. The results of Study 3 demonstrate that the type of stigma associated with entrepreneurial failure moderates the impact of impression-management strategies on the legitimacy of subsequent entrepreneurship. In situations where the stigma is attributable, implementing only assertive strategies is adequate to yield favorable results. However, in situations where the stigma is non-attributable, it is essential to implement both defensive and assertive strategies to effectively strengthen subsequent entrepreneurship legitimacy.Originality/valueThe findings make a valuable contribution to the existing literature on the recovery from entrepreneurial failure, and they also offer practical strategies for new ventures to adeptly handle the stigma of failure and resume their entrepreneurial endeavors.
HR analytics and AI adoption in IT sector: reflections from practitioners
Authors: Sharma Pooja , Bhattacharya Sonali , Bhattacharya Sanjay 
Published: February 2025

PurposeThis research paper explores the adoption and impact of Human Resource (HR) Analytics and Artificial Intelligence (AI) in the Information Technology (IT) sector. The study involves interviews with HR experts in the IT industry to understand their perceptions and experiences with these technologies.Design/methodology/approachData were collected through semi-structured interviews, in which fifteen HR managers were interviewed.FindingsThe findings reveal that HR Analytics and AI significantly impact HR functions, capabilities, and decision-making in the IT sector. To successfully adopt HR analytics and AI, HR professionals must possess technical skills such as data analysis, coding, analytical thinking, design thinking, and domain knowledge. The interviewees also highlighted the importance of connecting HR initiatives with financial outcomes, creating strategies, contributing to decision-making processes, and aligning activities with organizational objectives.Originality/valueThis paper incorporates insights on challenges in adopting analytics and AI in the HR domain along with strategies to overcome.
The influence of global value chain governance on supply network resilience
Authors: Statsenko Larissa , Scholten Kirstin , Stevenson Mark 
Published: February 2025

Purpose
Diversity – or having a range of different options – is an important part of being resilient. Yet research has not considered how diversity in terms of the governance relationship types that exist within a supply base or across a supply network relates to resilience. By drawing on a well-established global value chain (GVC) governance framework, this paper aims to investigate how different relationship governance types influence resilience at the dyadic and supply network level.

Design/methodology/approach
This research draws on 27 embedded cases of buyer-supplier relationships within a network, studied through 20 interviews in 11 organizations across four tiers of the Australian Defence Force supply network, including the end customer perspective, during and after a large-scale supply chain (SC) disruption. Analysis is conducted at the individual dyad and aggregated network levels.

Findings
At the dyadic buyer-supplier level, a variety of different resilience strategies and practices are used across the relationship governance types. Consequently, at the network level, relationships characterized by market and relational governance created more vulnerabilities during COVID-19 than hierarchical and modular governance relationships.

Originality/value
The GVC framework is extended to the SC domain, providing a deeper understanding of how GVC governance types in SC relationships relate to resilience strategies at the dyadic and network levels. Given that different governance relationships draw on different resilience strategies, diversity in governance relationships helps enhance overall resilience. Meanwhile, the findings show that resilience requires relational aspects to be considered alongside economic aspects of the GVC.
Environmentally responsible tourist behavior in socially responsible urban destinations
Authors: García-Maroto Inmaculada , Higueras-Castillo Elena , Muñoz-Leiva Francisco , Liebana-Cabanillas Francisco 
Published: February 2025

Purpose
The purpose of this study is to analyze environmentally responsible tourist behavior in a socially responsible destination. Specifically, this study explores the relationship between destination social responsibility and environmentally responsible behavior (ERB) using the stimulus–organism–response model as a theoretical framework.

Design/methodology/approach
The research sample is composed of Spanish tourists over 18 years of age who have visited one of the selected urban tourist destinations: Malaga, Barcelona, Madrid, Palma de Mallorca and Bilbao; therefore, all participants confirmed having visited one or more of the above destinations and coming from a different place, thus confirming that in no case were they residents. Data collection used a non-probabilistic sampling method with quotas reflecting the demographic makeup of the Spanish population. Information was gathered in July 2023 through an online survey using a structured questionnaire. Before the main study, a pilot investigation ensured the reliability and clarity of the measurement scales.

Findings
It is concluded that the proposed behavior model is satisfactory and allows for explaining the ERB of tourists, whose main determinants are place attachment, perception of the destination’s social responsibility, environmental concern and perceived effectiveness.

Originality/value
The main contribution expected from this study is the proposal of a conceptual model, in the environment of socially responsible destinations, that allows for the completion of the proposals made in the specific literature on ERB in the context of urban tourism.
A study of customer satisfaction with Slovakian spa companies: an importance-performance analysis
Authors: Košíková Martina , Šenková Anna , Kolesárová Stela , Šambronská Kristína 
Published: February 2025

PurposeThe aim of the contribution is to present the results of research that dealt with customers’ perception of the quality and range of services provided by spa companies in Slovakia.Design/methodology/approachThe primary data were obtained using a questionnaire which evaluated customer satisfaction with the services provided by spa companies in Slovakia at the beginning of 2020, before the outbreak of the COVID-19 pandemic. The research sample consisted of 863 customers of spa companies. Importance-performance analysis and other methods of inductive statistics were used to process the results.FindingsCustomer satisfaction with the quality of services provided is generally at a high level. Spa companies should pay more attention to the quality of catering services for patient groups A (the patient is reimbursed by the health insurance company for medical care, accommodation and meals at the spa) and B (the patient is only reimbursed by the health insurance company for healthcare) and to better planning of procedure dates for self-payers. When deciding on the choice of a spa company, the focus of the spa on the treatment of a specific diagnosis, and after this, the accessibility of transport, turned out to be the most common reasons.Research limitations/implicationsLimits of IPA analysis related to the measurement of performance and also the importance of individual attributes based on respondent preferences. The length of the survey increases. Low discriminatory power of respondents who may consider all attributes important.Originality/valueThe authors designed a novel questionnaire to identify the impact of individual factors on the level of services provided. Through IPA analysis, companies can precisely determine the services on which they should focus their attention in the future.
Addressing Staffing Shortages in Nursing Homes: Does Relaxing Training and Licensing Requirements Increase Nurse Aide Staffing?
Authors: Mehboob Gulrukh , Sharma Hari 
Published: February 2025

ABSTRACTObjectiveTo evaluate whether COVID‐19‐related nurse aide training and licensing relaxation policies improved staffing shortages in nursing homes.Study Setting and DesignStaffing shortages have been a long‐standing concern in nursing homes, and states are experimenting with different approaches to enhance nurse aide staffing. We use the latest quasi‐experimental difference‐in‐differences methods to evaluate the effect of relaxing training and licensing requirements in 19 states (treatment group) relative to the 31 states that did not implement such policies (control group). We analyze the combined effect of relaxing both training and licensing requirements, as well as the impact of relaxing each policy separately.Data Sources and Analytic SampleWe obtain quarterly data on nursing home characteristics, including adjusted nurse aide hours per resident day (HPRD) from 2019 to 2023 from Care Compare, a federal website with quality information on all Medicare/Medicaid‐certified nursing homes. After excluding outliers of staffing data (nurse aide HPRD > 5.25, or nurse aide HPRD = 0), our final analytical sample had 278,170 observations.Principal FindingsThe average nurse aide HPRD is 2.30 in the treatment group and 2.26 in the control group. Using the difference‐in‐differences regression analyses, we find no significant effect of the relaxation of training and licensing requirements on nurse aide levels (average treatment effect: −0.0001; p = 0.99). Similarly, separate analyses of training and licensing relaxation policies suggest that neither policy significantly impacts nurse aide staffing. Results are consistent when we adjust for staffing requirements, wage increase policies, and nursing home characteristics.ConclusionsOur findings suggest that the relaxation of training and licensing requirements may not lead to improved nurse aide staffing levels in nursing homes. Policymakers need to consider other strategies to address persistent staffing shortages in nursing homes.
Intraoperative opioid administrations, rescue doses in the post‐anaesthesia care unit and clinician‐perceived factors for dose adjustments in adults: A Danish nationwide survey
Authors: Tran Trang Xuan Minh , Wetterslev Mik , Nørskov Anders Kehlet , Meyhoff Christian Sylvest , Olsen Markus Harboe , Itenov Theis Skovsgaard , Mathiesen Ole , Karlsen Anders Peder Højer ,   The OPI•AID Collaborator Group
Published: February 2025

AbstractBackgroundThe impact of demographic‐ and surgical factors on individual perioperative opioid requirements is not fully understood. Anaesthesia personnel adjust opioid administrations based on their own clinical experience, expert opinions and local guidelines. This survey aimed to assess the current practice of anaesthesia personnel regarding intraoperative opioid treatment for postoperative analgesia and rescue opioid dosing strategies in the post‐anaesthesia care unit in Denmark.MethodsWe conducted a cross‐sectional online survey with 37 questions addressing pain management and opioid‐dosing strategies. Local site investigators from 46 of 47 public Danish anaesthesia departments distributed the survey. Data collection took place from 5 February to 30 April 2024.ResultsOf the 4187 survey participants, 2025 (48%) answered. Intra‐ and postoperative opioid doses were adjusted based on chronic pain, age, preoperative opioid use, body weight and type of surgery. Between 84% and 89% of respondents adhered to and had perioperative pain management guidelines available. Respondents preferred intraoperative fentanyl (44%) and morphine (36%) to prevent postoperative pain. Median intraoperative intravenous morphine equivalents ranged from 0.12 to 0.38 mg/kg in clinical scenarios. In these cases, the following variables were assembled in different combinations to assess their impact on dosing: age (30 vs. 65 years), sex (female vs. male), ASA score (1 vs. 3) and type of surgery (anterior cruciate ligament vs. laparoscopic cholecystectomy surgery). Respondents preferred intravenous morphine and fentanyl for moderate and severe postoperative pain, respectively. Median postoperative rescue doses were 0.06–0.12 mg/kg in clinical scenarios based on shifting combinations of the variables: age (30 vs. 65 years), ASA score (1 vs. 3) and degree of expected pain (moderate vs. severe).ConclusionRespondents preferred fentanyl and morphine for postoperative pain control with considerable variation in choice of opioid and morphine equivalent dose. Respondents expressed that guidelines were highly available and strongly adhered to. Opioid dosing was predominantly guided by chronic pain, age, preoperative opioid use, body weight and type of surgery.
Patterns of Intrafruit Seed Abortion and Variation in Seed Mass of the Guanacaste Tree (Enterolobium cyclocarpum, Fabaceae) in Populations From Mexico and Costa Rica
Authors: Lobo Jorge A. , Cristóbal‐Pérez Edson J. , Chavarría María M. , Quesada Mauricio 
Published: February 2025

ABSTRACTAnalyses of the variation in the abortion rate and mass of seeds of tropical trees are scarce, despite their importance in modulating seed production, plant recruitment, and herbivore foraging patterns. We studied these reproductive processes in a tropical dry forest tree species. We studied the patterns of intrafruit abortion and seed mass in fruits of the Guanacaste tree, Enterolobium cyclocarpum, in samples of trees from 5 populations in Mexico and Costa Rica. We analyzed the effects of developmental phase, fruit and maternal tree effects, and seed position within the fruit on the abortion rate and mass of seeds from mature and immature fruits. Naturally pollinated flowers were studied to determine the patterns of ovule fertilization within the ovaries. Overdispersion of the abortion rate per fruit within trees and a higher frequency of abortions in the extreme positions of the fruit were common patterns in all populations. These patterns were observed from the beginning of fruit formation. Ovules in the stylar position do not have higher survival rates, despite their precedence in fertilization. A negative relationship was found between abortion events and seed mass. Fruit‐specific fertilization and developmental conditions seem to be the main factors driving the fruit abortion rate and seed mass, causing high variability in seed survival and mass within maternal trees. The correlation between the fruit abortion rate and seed mass may be related to the capacity of the fruit to drain maternal resources. These patterns are common to all studied Guanacaste tree populations.