bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of output feedback proportional–integral controller for polynomial fuzzy systems
Authors: Radmanesh Hamid Reza , Moarefianpour Ali , Jahed‐Motlagh Mohammad Reza 
Published: July 2022

AbstractIn this paper, the design of dynamic output feedback control law for polynomial fuzzy systems is studied. The main purpose is to design a tracker control law for fuzzy polynomial systems that are subject to external bounded disturbances. The structure of the control law is considered as fuzzy proportional–integral control. The conditions for deriving the control law are formulated in the form of a sum of square (SOS) feasibility problem. The designed control law will be able to force the system state vector to follow the state vector of a stable reference model in addition to guaranteeing the
performance measure. The simulation results show the efficiency of the proposed method exposed to external disturbance.
Determination of the biochemical methane potential of food waste after aerobic storage and aeration pretreatment
Authors: Oliveira Bruna Patrícia de , Sganzerla William Gustavo , Léo Patricia , Forster‐Carneiro Tânia , Martins Gilberto 
Published: July 2022

AbstractFood waste represents a relevant fraction of municipal solid organic waste, and anaerobic digestion can be an eco‐friendly alternative for sustainable management in a circular economy framework. One of the challenges to the implementation of anaerobic digestion is the availability of easily degradable compounds in the substrate, which can be solved by the application of pretreatment to increase methane production. In this study, the influence of aerobic storage time and forced continuous aeration pretreatment on the biochemical methane potential of food waste was evaluated. The results demonstrate an increase in the methane production concerning the total volatile solids (TVS) of food waste stored for 7 days (425 NmL CH4 g−1 TVS) compared with fresh samples without pretreatment (375 NmL CH4 g−1 TVS). The adoption of forced and continuous aeration pretreatment on food waste for 4 days produced 456 NmL CH4 g−1 TVS for the leachate, 1.22‐fold higher than that for the food waste without storage. In conclusion, the application of aeration pretreatment previous to anaerobic digestion can be an alternative to increase the methane potential from food waste. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
Flexible customization of the self‐assembling abilities of short elastin‐like peptide Fn analogs by substituting N‐terminal amino acids
Authors: Suyama Keitaro , Shimizu Marin , Maeda Iori , Nose Takeru 
Published: July 2022

AbstractElastin‐like peptides (ELPs) are thermoresponsive biopolymers inspired by the characteristic repetitive sequences of natural elastin. As ELPs exhibit temperature‐dependent reversible self‐assembly, they are expected to be biocompatible thermoresponsive materials for drug delivery carriers. One of the most widely studied ELPs in this field is the repetitive pentapeptide, (VPGXG)n. We previously reported that phenylalanine‐containing ELP (Fn) analogs, in which the former Val residue of the repetitive sequence (VPGVG)n is replaced by Phe, show coacervation with a short chain length (n = 5). Owing to their short sequences, Fn analogs are easily modified in amino acid sequences via simple chemical synthesis, and are useful for investigating the relationship between peptide sequences and temperature responsiveness. In this study, we developed Fn analogs by replacing Phe residue(s) with other amino acids or introducing another amino acid at the N‐terminus. The temperature responsiveness of the Fn analogs changed drastically with the substitution of a single Phe residue, suggesting that aromatic amino acids play an important role in their self‐assembly. In addition, the self‐assembling ability of Fn was enhanced by increasing the bulkiness of the N‐terminal amino acids. Therefore, the N‐terminal residue was considered to be important for hydrophobicity‐induced intermolecular interactions between the peptides during coacervation.
Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process
Authors: Gutierrez‐Valdes Noemi , Häkkinen Suvi T. , Lemasson Camille , de Groot Jonas , Ele‐Ekouna Jean‐Pierre , Guillet Marina , Cardon Florian , Ritala Anneli 
Published: July 2022

AbstractHairy root systems have proven to be a viable alternative for recombinant protein production. For recalcitrant proteins, maximizing the productivity of hairy root cultures is essential. The aim of this study was to optimize a Brassica rapa rapa hairy root process for secretion of alpha‐
l‐iduronidase (IDUA), a biologic of medical value. The process was first optimized with hairy roots expressing eGFP. For the biomass optimization, the highest biomass yields were achieved in modified Gamborg B5 culture medium. For the secretion induction, the optimized secretion media was obtained with additives (1.5 g/l PVP + 1 mg/l 2,4‐
d + 20.5 g/l KNO3) resulting in 3.4 fold eGFP secretion when compared to the non‐induced control. These optimized conditions were applied to the IDUA‐expressing hairy root clone, confirming that the highest yields of secreted IDUA occurred when using the defined additive combination. The functionality of the IDUA protein, secreted and intracellular, was confirmed with an enzymatic activity assay. A > 150‐fold increase of the IDUA activity was observed using an optimized secretion medium, compared with a non‐induced medium. We have proven that our B. rapa rapa hairy root system can be harnessed to secrete recalcitrant proteins, illustrating the high potential of hairy roots in plant molecular farming.
A course‐based undergraduate research experience to illustrate the early stages of the drug discovery process
Authors: García‐Caballero Melissa , Moya‐García Aurelio , Torres‐Vargas José Antonio , García‐Ponce Ángel Luis , Rodríguez‐Quesada Ana 
Published: July 2022

AbstractWe have implemented at the University of Málaga (Spain) a new course‐based undergraduate research experience (CURE) to involve undergraduate students of Science in a real‐world scientific problem. Within the topic “Let's find acetylcholinesterase inhibitors as new drug candidates for the treatment of Alzheimer's”, students have been engaged into the early stages of the drug discovery process. Working in groups of 4–5 persons, they have searched information in databases, proposed solutions to the driving question and designed protocols to carry them out in vitro and in silico. Overall, the implementation of this experience has been very satisfactory in terms of academic performance and students' perception. This article reports a session from the virtual international 2021 IUBMB/ASBMB workshop, “Teaching Science on Big Data”.
Prediction of pathological complete response to neoadjuvant chemotherapy in patients with breast cancer using a combination of contrast‐enhanced ultrasound and dynamic contrast‐enhanced magnetic resonance imaging
Authors: Han Xue , Yang Huajing , Jin Shiyang , Sun Yunfeng , Zhang Hongxia , Shan Ming , Cheng Wen 
Published: July 2022

AbstractThis study aimed to evaluate the value of dynamic contrast‐enhanced ultrasound (CEUS) combined with dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI) in predicting pathological complete response (pCR) in patients with breast cancer receiving neoadjuvant chemotherapy (NAC). Fifty‐seven female patients with breast cancer (mean age, 50.46 years; range, 32–66 years) scheduled for NAC were recruited. CEUS and DCE‐MRI were performed before and after NAC. Imaging features and their changes were compared with postoperative pathological results. After the clinical differences were balanced using propensity score matching, univariate and multiple logistic regression analyses were used to derive the characteristics independently associated with pCR. Receiver operating characteristic curve analysis was performed to assess diagnostic performance. After six to eight cycles of NAC, 24 (42.1%) patients achieved pCR, while 33 (57.9%) did not. Multivariate analysis showed that enhancement order on CEUS and DCE‐MRI before NAC, reduction in diameter and enhancement shape on CEUS, maximum diameter on DCE‐MRI, and the type of progressive dynamic contrast enhancement after NAC were independently associated with pCR after NAC. The area under the receiver operating characteristic curve for CEUS+DCE‐MRI was 0.911 (95% confidence interval, 0.826–0.997), and the specificity and positive predictive values were 87.0% and 87.5%. CEUS and DCE‐MRI have the potential for assessing the pathological response to NAC in patients with breast cancer; their combination showed the best diagnostic performance. CEUS+DCE‐MRI has proved beneficial for comprehensive assessment and personalizing treatment strategies for patients with breast cancer.
Adolescent and young adult (AYA) oncology: A credentialed area of focused competence in Canada
Authors: Srikanthan Amirrtha , Karpinski Jolanta , Gupta Abha 
Published: July 2022

AbstractBackgroundAdolescents and young adults (AYA, ages 15–39 years) affected by cancer have unique treatment, survivorship, and palliation concerns. Current oncology training does not focus on the distinctive needs of this demographic. Amid this recognition, the Canadian National AYA Cancer Task Force and Canadian Partnership Against Cancer have advocated the need for clinicians with formalized AYA experience. To address this need and standardize training, a national task force developed criteria for structured academic programs in AYA Oncology in Canada.MethodsWorkshops were organized to identify and establish the fundamentals of practice in AYA Oncology through consensus. These workshops followed the pre‐existing rigorous process established by the Royal College of Physicians and Surgeons of Canada (Royal College) for new program development. The process includes: (i) developing the tasks associated with the discipline's practice, (ii) identifying the evidence trainees must provide to demonstrate tasks can be performed independently (the competence portfolio), (iii) developing training requirements and summarizing the knowledge, skills and attitudes required to perform these tasks, and (iv) identifying specific experiences essential to acquiring skills and demonstrating competent performance.ResultsAYA Oncology is a recognized an Area of Focused Competence (AFC) by the Royal College.ConclusionThe AFC designation in AYA Oncology provides a standardized curriculum, training experience and accreditation process to attract oncologists, promote expertise and advance AYA oncology care.
A hierarchy of sustainable grocery shopping behaviours: Using Rasch modelling to explore adoption groups
Authors: Ganglmair‐Wooliscroft Alexandra , Wooliscroft Ben 
Published: July 2022

AbstractGrocery shopping is a major contributor to unsustainable consumption in the developed world. This study constructs a hierarchy of sustainable grocery shopping (SGS), using a broad range of SGS consumption activities that contribute to an individual's aggregate grocery shopping. We confirm the suitability of Rasch modelling to construct the SGS hierarchy and, recognize the definitional equivalence between features of the Rasch model and Rogers' adoption groups. Our results are transformed into theoretically founded, empirically observed SGS adoption segments. SGS adoption groups are analysed with reference to multiple research streams. Our results show that the diffusion of SGS behaviours in a population is in its early stages and most SGS adoption segments undertake a limited number of SGS behaviours. Demographic characteristics do not contribute to explaining SGS membership, but personal values do. Personal values are also related to the observation of spill‐over between items of similar behavioural difficulty, rather than within product categories. Only the most advanced SGS adoption groups consider sustainably sourced food as a decision criteria. We show the importance of investigating SGS with a systemic approach.
Design, Synthesis and Biological Evaluation of Novel Spiro‐[Chroman‐2,4′‐Piperidin]‐4‐One Analogs as Anti‐Tubercular Agents
Authors: Chitti Surendar , Nandikolla Adinarayana , Khetmalis Yogesh Mahadu , Van Calster Kevin , Kumar Boddupalli Venkata Siva , Kumar Banoth Karan , Murugesan Sankaranarayanan , Cappoen Davie , Sekhar Kondapalli Venkata Gowri Chandra 
Published: July 2022

AbstractA series of novel spiro‐[chromane‐2,4′‐piperidin]‐4(3H)‐one derivatives were designed, synthesized and structures were confirmed by analytical methods, viz., 1H‐NMR, 13C‐NMR and mass spectrometry. The synthetic derivatives were evaluated for their anti‐tuberculosis (anti‐TB) activity against Mycobacterium tuberculosis (Mtb) strain H37Ra. Among all the evaluated Compounds, PS08 exhibited significant inhibition with MIC value of 3.72 μM while MIC values of the remaining Compounds ranged from 7.68 to 230.42 μM in comparison to the standard drug INH (MIC 0.09 μM). The two most active Compounds however showed acute cytotoxicity towards the human MRC‐5 lung fibroblast cell lines. The in silico ADMET profiles of the titled Compounds were predicted and found within the prescribed limits of the Lipinski and Jorgenson rules. Molecular docking study of the notably active Compound (PS08) was also carried out after performing validation in order to understand the putative binding position of the test ligand at the active site of selected target protein Mtb tyrosine phosphatase (PtpB).
Rapid Aging of Binder‐Free Zeolite NaY Beads in Thermal Energy Storage Applications
Authors: Auer Bernhard , Rönsch Stefan 
Published: July 2022

AbstractZeolites can be used to store thermal energy, but they may age and lose a fraction of their storage capacity during prolonged use. The purpose of this study is to determine which parameters are responsible for the aging of the zeolite NaY by exposing it to extreme hydrothermal conditions. In a laboratory unit, a fixed bed of binder‐free zeolite NaY beads was treated hydrothermally, and the beads were analyzed for signs of aging. Also, a fixed bed of binder‐free zeolite 13X beads was tested for comparison. The results indicate that the two zeolite types age differently, depending on temperature and time of exposure.
Hilbert‐Huang Transform Analysis of the Concentration Field of a Two‐Layer Impinging Stream Mixer
Authors: Zhang Jianwei , He Ziyu , Sha Xinli , Feng Ying , Dong Xin 
Published: July 2022

AbstractThe concentration field of a two‐layer impinging stream mixer was measured by the plane laser‐induced fluorescence (PLIF) technology, and the energy distribution of the concentration field was studied by Hilbert‐Huang transform. The Hilbert spectrum and marginal spectrum under different operational conditions were analyzed in the mixer. It is indicated that the energy of the concentration field firstly increased and then decreased with larger nozzle diameter and nozzle spacing. The energy of the concentration field incremented with the rise of the jet Reynolds number. According to the energy characteristic value Pi, the flow field of the impinging stream mixer was divided into three regions. It could provide a quantitative basis for identification of the flow regimes of impinging steam.
Exploring the Electrochemistry of Iron Dithiolene and Its Potential for Electrochemical Homogeneous Carbon Dioxide Reduction
Authors: Armstrong Craig G. , Potter Mark , Malcomson Thomas , Hogue Ross W. , Armstrong Sapphire M. , Kerridge Andrew , Toghill Kathryn E. 
Published: July 2022

AbstractIn this work, the dithiolene complex iron(III) bis‐maleonitriledithiolene [Fe(mnt)2] is characterised and evaluated as a homogeneous CO2 reduction catalyst. Electrochemically the Fe(mnt)2 is reduced twice to the trianionic Fe(mnt)23− state, which is correspondingly found to be active towards CO2. Interestingly, the first reduction event appears to comprise overlapping reversible couples, attributed to the presence of both a dimeric and monomeric form of the dithiolene complex. In acetonitrile Fe(mnt)2 demonstrates a catalytic response to CO2 yielding typical two‐electron reduction products: H2, CO and CHOOH. The product distribution and yield were governed by the proton source. Operating with H2O as the proton source gave only H2 and CO as products, whereas using 2,2,2‐trifluoroethanol gave 38 % CHOOH faradaic efficiency with H2 and CO as minor products.
Methyl Carbamate‐Lithium Salt Deep Eutectic Electrolyte for Lithium‐Ion Batteries
Authors: Hardin Nathaniel Z. , Duca Zachary , Imel Adam , Ward Patrick A. 
Published: July 2022

AbstractDeep eutectic electrolytes (DEEs) represent a burgeoning field in electrolytes for energy storage applications. Compared to the more conventionally studied Li‐ion electrolyte systems, these electrolytes offer numerous advantages, such as high ion concentration, costs, lower temperature operation, and safety. In this work, the formation of a novel eutectic electrolyte based on lithium salts and methyl carbamate was reported. These DEEs were formed via mixing lithium salts and low‐cost methyl carbamate at ratios varying from 1 : 2 to 1 : 5 (mol:mol). The DEE formed from methyl carbamate and lithium hexafluorophosphate (LiPF6) at a 1 : 5 molar ratio generated a 4V stability window and a 25 °C conductivity of up to 3.16E−3 S cm−1. The DEE formed from methyl carbamate and lithium bis(trifluoromethane)sulfonimide (LiTFSI) at a 1 : 5 molar ratio generated a 3.2 V stability window and a 25 °C conductivity of up to 2.87E−3 S cm−1. The LiPF6 DEE also demonstrated a discharge capacity of 128 mAh g−1after 50 cycles with 93 % discharge capacity retention at moderate cycling rates of 0.5C in a lithium titanate (LTO)/lithium iron phosphate (LFP) full cell. These results demonstrate the potential for methyl carbamate to generate eutectics from lithium salts and offer alternative electrolytes for use in low‐temperature Li‐ion battery applications.
Pragmatic randomized trial assessing the impact of digital health technology on quality of life in patients with heart failure: Design, rationale and implementation
Authors: Victoria‐Castro Angela M. , Martin Melissa , Yamamoto Yu , Ahmad Tariq , Arora Tanima , Calderon Frida , Desai Nihar , Gerber Brett , Lee Kyoung A. , Jacoby Daniel , Melchinger Hannah , Nguyen Andrew , Shaw Melissa , Simonov Michael , Williams Alyssa , Weinstein Jason , Wilson Francis P. 
Published: July 2022

AbstractBackgroundSelf‐care and patient engagement are important elements of heart failure (HF) care, endorsed in the guidelines. Digital health tools may improve quality of life (QOL) in HF patients by promoting care, knowledge, and engagement. This manuscript describes the rationale and challenges of the design and implementation of a pragmatic randomized controlled trial to evaluate the efficacy of three digital health technologies in improving QOL for patients with HF.HypothesisWe hypothesize that digital health interventions will improve QOL of HF patients through the early detection of warning signs of disease exacerbation, the opportunity of self‐tracking symptoms, and the education provided, which enhances patient empowerment.MethodsUsing a fully electronic enrollment and consent platform, the trial will randomize 200 patients across HF clinics in the Yale New Haven Health system to receive either usual care or one of three digital technologies designed to promote self‐management and provide critical data to clinicians. The primary outcome is the change in QOL as assessed by the Kansas City Cardiomyopathy Questionnaire at 3 months.ResultsFirst enrollment occurred in September 2021. Recruitment was anticipated to last 6–8 months and participants were followed for 6 months after randomization. Our recruitment efforts have highlighted the large digital divide in our population of interest.ConclusionAssessing clinical outcomes, patient usability, and ease of clinical integration of digital technologies will be beneficial in determining the feasibility of the integration of such technologies into the healthcare system.
Laser Capture Microdissection Optimization for High‐Quality RNA in Mouse Brain Tissue
Authors: Nogueira Margareth , Golbert Daiane C. F. , Landeira Bruna , Leão Richardson N. 
Published: July 2022

AbstractLaser Capture Microdissection (LCM) is a method that allows one to select and dissect well‐defined structures, specific cell subpopulations, or even single cells from different types of tissue for subsequent extraction of DNA, RNA, or proteins. Its precision allows the dissection of specific groups of cells, avoiding unwanted cells. However, despite its efficiency, several steps can affect the sample RNA integrity. RNA instability represents a challenge in the LCM method, and low RNA integrity can introduce biases, as different transcripts often have different degradation rates. Here we describe an optimized protocol to provide good‐concentration and high‐quality RNA from specific structures: dentate gyrus and CA1 in the hippocampus, basolateral amygdala, and anterior cingulate cortex of mouse brain tissue. However, the protocol is applicable to other areas of interest. © 2022 Wiley Periodicals LLC.Basic Protocol: Laser capture microdissection of mouse brain tissue
Breeding increases grain yield, zinc, and iron, supporting enhanced wheat biofortification
Authors: Govindan Velu , Atanda Sikiru , Singh Ravi P. , Huerta‐Espino Julio , Crespo‐Herrera Leonardo Abdiel , Juliana Philomin , Mondal Suchismita , Joshi Arun K. , Bentley Alison R. 
Published: July 2022

AbstractEstimation of the rate of genetic gain over time allows quantification of breeding progress. Here we report on the rate of grain yield and zinc (Zn) concentration increase over 11 yr of targeted wheat (Triticum aestivum L.) biofortification breeding at the International Maize and Wheat Improvement Center (CIMMYT). Data from yield trials evaluated across multiple locations in South Asia and beyond showed that average annual increases in grain yield potential of ∼1.5% and 0.9% per year gains for grain Zn and Fe concentrations. Across locations in all countries, mean yields of the five highest‐yielding entries showed an annual gain of 109 kg ha−1 yr−1 in yield and 0.3 mg kg−1 for grain Fe and Zn as well as 0.66 g for the yield component thousand‐grain weight. There was a strong positive correlation between Fe and Zn (r = .42) across locations, whereas no genetic correlation was observed between grain yield and Zn (r = .05) across locations. Despite the slight negative relationship between yield and Zn, through targeted crossing and development of large segregating populations we were able to identify transgressive segregants combining increased yield and Zn concentrations. Significant differences between lines for grain micronutrient concentrations were detected, and significant location effects on grain Zn and Fe concentrations were observed. These results demonstrate that continuous and simultaneous genetic gain for grain yield and concentrations of Fe and Zn is possible in elite spring bread wheat lines with potential to deliver global impact through identification of superior parents for use by national breeding programs and the release of biofortified wheat cultivars.