bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Clustering and Distribution of Heavymetal Accumulation and Translocation as an Ability of Mangrove Vegetation to Reduce Impact of Heavymetal (Hg, Cd and Zn) Pollution
Authors: Hilmi Endang , Dewi Rose , Sudiana Eming , Mahdiana Arif , Sari Lilik Kartika , Cahyo Tri Nur 
Published: March 2022

Abstract
Mangrove has ability to reduce impact of heavy metal contaminant which released from industry and domestic waste. Mangrove has ability to absorb, accumulate, and translocate heavy metal contaminant. Whereas the clustering and landscape of mangrove ecosystem show the grading and zoning of mangrove vegetation to accumulate heavymetal pollution. .Therefore, this study aimed to analyze distribution and clustering of heavy metal accumulation and traslocation in mangrove ecosystem.. The results showed the accumulation of Mercury were 0.1347 ± 0.0212 ppm (sediment), 0.0137 ± 0.0137 ppm (water), 0.0110–0.0640 ppm (stem and bark), 0.0020–0.0120 ppm (leaves), and 0.0260–0.0690 ppm (roots). Cadmium accumulations between 1.6048 ± 0.3595 ppm (sediment), 0.0292 ± 0.0047 ppm (water), 0.0790–0.2650 ppm (stem and bark), 0.0130–0.0480 ppm (leaves), and 0.1560–0.2300 ppm (roots). Furthermore, Zinc had score that were 35.1746 ± 5.4856 ppm (sediment), 0.0550 ± 0.0095 ppm (water), 10.3250–32.4490 ppm (stem and bark), 7.1200-23.4150 ppm (leaves), and 16.7170–39.1200 ppm (roots). However, bioaccumulation factors of Hg ranged between 0.0210–0.4751, Cd 0.0105–0.1541, and Zn 0.2807–1.2506 with the translocation factors of 0.0459–1.0547 (Hg), 0.0642–1.3855 (Cd), and 0.4086–1.3057 (Zn). The mangrove landscape showed that of Sonneratia alba (best of Hg accumulation), Rhizophora mucronata (best of Cd accumulation), Avicennia marina (best of Zn accumulation)
The variable clinical spectrum of nephroblastomatosis – results from the German Society of Pediatric Oncology and Hematology (GPOH) childhood kidney tumor group.
Authors: Furtwängler Rhoikos , Schenk Jens-Peter , Friesenbichler Waltraud , Gessler Manfred , Wagenpfeil Stefan , Kager Leo , Müller Marina , Welter Nils , Fuchs Jürgen , Warmann Steven , Meier Clemens M , Hubertus Jochen , Rübe Christian , Vokuhl Christian , Melchior Patrick , Graf Norbert 
Published: March 2022

Abstract
Background: Histologically nephroblastomatosis consists of ≥2 microscopic foci of nephrogenic rests. In contrast, clinically apparent nephroblastomatosis consists of single, multiple or diffuse hyperplastic nephrogenic rests (HNR) that can be mistaken for nephroblastoma on imaging. Their treatment and outcome are as variable as published results.Methods: Analysis of 78 patients having clinically apparent uni- or bilateral nephroblastomatosis out of 2347 patients registered for a renal tumor in the consecutive International Society of Pediatric Oncology (SIOP)93-01/GPOH and SIOP2001/GPOH studies between 1993 and 2014. Results: Median age at diagnosis and follow-up was 13 months (0–12.7 years) and 9.2 years, respectively. 59% of patients were female, 57% had bilateral and 53% multifocal or diffuse nephroblastomatosis. Radiologic diagnosis was conclusive in 67% when reviewed centrally versus 30% without review (p=0.015). 27% of patients developed nephroblastoma(s). On histology 45% were high-risk, 30% intermediate-risk and 25% upfront-surgery-blastemal subtype. 5-year-event-free (EFS), nephroblastoma-free-(NFS) and overall-survival (OS) were 60.7%, 79.8% and 95.5%, respectively. Univariate risk factors for EFS/NFS were female gender (p<0.001/p=0.031), bilateral (p=0.002/p=0.03) and diffuse HNR at diagnosis (p<0.001/p=0.016), and non-complete remission (non-CR) at the end of first-line-treatment (both p<0.001). Gender and non-CR remained significant in multivariate COX regression analysis for EFS (p=0.005 and <0.001, respectively), non-CR also for NFS (p=0.002). 34 of 74 evaluable patients achieved CR within 12 weeks after diagnosis, 25 of them had no further treatment and were followed, 9 received chemotherapy. Their EFS was 78% and 100%, respectively.Conclusion: Nephroblastomatosis confronts clinicians with a variety of pitfalls, but overall survival is excellent. However, patients need an extended follow-up for more than a decade. In patients who have achieved CR within 12 weeks, a relatively short treatment is sufficient. Contrary, the risk of progression for patients having bilateral and diffuse HNR is significantly increased, despite prolonged treatment. A clear common terminology is needed for future trials to facilitate true comparability of results.
Intact observation of secondary xylem and parenchyma cells in woody plants using a combination of scanning electron microscopy and broad argon ion beam milling
Authors: Hatano Tomohiro , Nakaba Satoshi , Horikawa Yoshiki , Funada Ryo 
Published: March 2022

Abstract
The secondary xylem of woody plants consists of fragile living cells and rigid cell walls. However, xylem structures are easily damaged during mechanical cross-sectioning for electron microscopy analysis. A broad argon ion beam (BIB) milling technique is commonly employed for analysis using scanning electron microscopy (SEM) in material science to generate a large and distortion-free cross-section. However, BIB milling is rarely used in plant science. In this study, SEM combined with the BIB milling technique was validated as an accurate tool for structural observation of xylem tissues of two woody plants, Pinus densiflora (Japanese red pine) and Quercus phillyraeoides (Ubame oak), in comparison to cutting with the classical microtome. The BIB milling method does not require epoxy resin embedding because of prior chemical fixation and critical point drying of the sample, thus producing a three-dimensional image. The results showed that xylem structures were well-preserved in their natural state in the BIB-milled cross-section compared with the microtome cross-section. The observation using SEM with BIB milling was found to be useful for imaging hard and soft plant tissues, particularly the ultrastructure of fragile secondary xylem cells. Thus, we propose a new imaging method for detailed observation of plant tissue structures.
Pregnancy Outcomes and Associated Factors in Women with Cardiovascular Diseases
Authors: Wolie Aysheshim Geremew , Demas Tangute , Muzeyin Khadija , Yesuf Subah Abderehim , Tsegaye Tigist 
Published: March 2022

Abstract
Background: Worldwide, cardiovascular diseases are among the leading cause of death, representing about a third of all global deaths. Of these deaths, 85% were due to heart attack and stroke. While existing evidences reveal that pregnancy in women with cardiovascular disease is associated with a range of feto-maternal complications, corresponding studies among pregnant women in Ethiopian context are rather limited. Thus, the aim of this study was to assess pregnancy outcomes and associated factors in women with cardiovascular diseases.Methods: An institution-based cross-sectional study was conducted among 384 pregnant women with cardiovascular disease attending three selected public hospitals of Addis Ababa, Ethiopia. Women’s charts were selected with multi-stage sampling technique. The collected data was entered and analyzed using Statistical package for Social Science version 26. Descriptive and inferential statistics were used to analyze the data. A p value of <0.05 was predetermined to be statistically significant for multivariable logistic regression.Result: Two-thirds (255;66.4%) of the pregnant mothers were younger than 36 years. More than half (202;52.6%) had valvular heart disease and about a third (116;30.2%) had heart failure. There were 25.5% heart failure events and 18.7% life threatening arrhythmia episodes. Two hundred fifty-four (66.1%) of the cohort had poor pregnancy outcomes such as preterm delivery (39.6%), abortion (15.6%) and stillbirth (8.3%) of the pregnancies. 8.1% of the pregnancies ended with maternal death. Women with valvular heart disease (AOR=3.418, 95% CI =2.045-5.714) and heart failure (AOR =5.058, 95% CI=2.641-9.687) were more likely to have poor pregnancy outcome.Conclusion: Pregnancy in women with CVD is a serious cause of maternal mortality and morbidity in the study setting. These women should receive strict clinical attention by a multidisciplinary team both during preconception and prenatal period to improve feto-maternal outcomes.
Attentional processing of body images in women with overweight and obesity
Authors: Baur Julia , Krohmer Kerstin , Naumann Eva , Svaldi Jennifer 
Published: March 2022

Abstract
Purpose: Despite the claim to integrate body image interventions in obesity treatment, little is known about the mechanisms involved in maintaining body dissatisfaction in persons with overweight and obesity. Therefore, the present study sought to investigate attentional processing of body stimuli in women with overweight and obesity (OW). Methods: Women with OW (n = 82) and normal weight controls (NW; n = 44) conducted two eye-tracking paradigms. In the first paradigm, fixation duration on the subjectively most beautiful and ugliest body part of one’s own and a weight-matched control body were analyzed. In the second paradigm, picture pairs including the own and a control body or object were presented and initial fixation orientation was measured. Automatic and intentional processing of the body pictures was manipulated by either indicating on which side which stimuli would appear or not.Results: Women with OW displayed a bias towards the ugliest as opposed to the most beautiful body part, whereas women with NW showed a balanced viewing pattern. Furthermore, both groups showed a preference for bodies relative to the object. However, only women with OW preferred their own relative to the control body during intentional processing.Conclusion: Taken together, results point towards a self-focused and deficit-oriented gaze pattern in women with overweight and obesity. Targeting these processes might help to improve obesity treatment outcomes.Level of Evidence: Level I, experimental study.
Surgical outcomes of posterior cervical laminectomy with instrumented fusion managing regional kyphotic cervical spondylotic myelopathy and k-line negative ossification of the posterior longitudinal ligament: Is the decompression sufficient?
Authors: ZHENG Guoquan , HUANG Yi , WU Fangqian , LI Bing , HU Wenhao , WANG Tianhao , QI Dengbin , XUE Chao 
Published: March 2022

Abstract
Purpose To evaluate the efficiency of single-staged posterior cervical laminectomy with instrumented fusion (PLF) for patients with regional kyphotic cervical spondylotic myelopathy (KSM) and negative K-line ossification of the posterior longitudinal ligament (NKO).Methods Patients who were initially diagnosed as cervical stenosis with myelopathy with KSM or NKO were analyzed and compared retrospectively. Posterior shift of the spinal cord was measured comparing preoperative C3-C7 column-cord distance and that of the final follow-up in mid-sagittal MRI. Neurological status was evaluated by the Japanese Orthopaedic Association (JOA) score and recovery rate of JOA score. Postoperative complications were calculated and statistically analyzed. Results For KSM group (n = 12), the preoperative JOA score was 8.250±2.261 points, improved to 13.333±1.875 points at final follow-up. The JOA score improved from 8.421±1.575 points perioperatively to 12.316±1.493 points at final follow-up in NKO group (n = 19). The JOA score changes of both group were larger than 2.5 points of the minimum clinically important difference. The posterior shift of the spinal cord is 12.167±5.762 mm in KSM group and 9.600±6.124 in NKO group. No significant difference of the surgical outcomes was observed between the two group. 2 patients (16.7%) in KSM group had postoperative axial pain. 1 patient (8.3%) in KSM group and 2 patient (10.5%) in NKO group had C5 palsy.Conclusion PLF is safe and efficient for both KSM and KNO. There are similar surgical outcomes of regional kyphotic CSM and negative K-line OPLL after PLF while postoperative axial pain was more likely to happen in regional kyphotic CSM.
The Trend of Iatrogenic Genitourinary Fistula from 2005 to 2019 and Its Risk Factors at Addis Ababa Fistula Hospital, Addis Ababa, Ethiopia: A retrospective study
Authors: Ambaw Maranata Dawit 
Published: March 2022

Abstract
Background
Genitourinary fistula is one of a public health importance in low- and middle-income countries. It causes multiple health adversities. Mainly, lack of timely and appropriate intervention such as cesarean section causes genitourinary fistula. Nevertheless, iatrogenic genitourinary fistula is a result of medical error during obstetric and gynecological surgery such as while cesarean section and hysterectomy. This study examined the trend of iatrogenic genitourinary fistula, major surgical procedures leading to iatrogenic fistula and its risk factors.
Methods
a facility based cross sectional study design was deployed. The respondents were included retrospectively using their medical history from January 2005 to December 2019 at Addis Ababa fistula hospital, Addis Ababa, Ethiopia. Data was analyzed using SPSS version 20.0 software. Annual percent change and average annual percent change were calculated using joinpoint regression analysis on joinpoint regression program version 4.8.0.1. Bivariate and multivariable regression model was also undertaken to implore the important risk factors for iatrogenic genitourinary fistula.
Results
Total 9229 fistula patients treated in the hospital. Of which 643(6.96%) and 8,586(93.03%) referred iatrogenic genitourinary fistula and obstetric fistula cases, respectively. The mean age of iatrogenic genitourinary fistula patients was 30.02 (± 4.63 SD) years, ranged 17–45 years. Among the iatrogenic genitourinary fistula cases, 341(53%) were caused by cesarean section. Other causes were errors while repairing ruptured uterus 131(20.4), during undertaking hysterectomy for ruptured uterus 78(12.1%), and while gynecological hysterectomy 93(14.5%). From 2005 to 2019, there was a significant rising trend in iatrogenic genitourinary fistula (1.55–52.9%, p < 0.001). Average annual percent change from 2005 to 2019 was 13.2% (95% CI: 7.7, 18.9). Statistically significant risk factors for iatrogenic genitourinary fistula were history of obstetric or gynecologic surgery, type of institution where the surgery conducted, and parity.
Conclusion
The trend of IGF substantially increased while the OF declined over 2005 to 2019 period. Patient safety and quality surgical procedure on pelvic surgeries is paramount important. The study also revealed that CS was the most frequent surgery resulting IGF followed by repairing ruptured uterus, hysterectomy for ruptured uterus and gynecologic hysterectomy.
Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
Authors: Fan Rong , Liu Yang , DiStasio Marcello , Su Graham , Asashima Hiromitsu , Enninful Archibald , Qin Xiaoyu , Deng Yanxiang , Bordignon Pino , Cassano Marco , Tomayko Mary , Xu Mina , Halene Stephanie , Craft Joseph , Hafler David 
Published: March 2022

Abstract
We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 198 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 283 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.
Genetic dissection of resistance to stripe rust in current Central and Northern European winter wheat germplasm
Authors: Shahinnia Fahimeh , Geyer Manuel , Schürmann Friederike , Rudolphi Sabine , Holzapfel Josef , Kempf Hubert , Stadlmeier Melanie , Löschenberger Franziska , Morales Laura , Buerstmayr Hermann , Sánchez Julio Isidro y , Akdemir Deniz , Mohler Volker , Lillemo Morten , Hartl Lorenz 
Published: March 2022

Abstract
Stripe or yellow rust, caused by the fungus Puccinia striiformis Westend f. sp. tritici , is one of the most destructive wheat diseases. Sustainable management of wheat stripe rust can be achieved through the deployment of rust resistant cultivars. To detect effective resistance loci for use in breeding programs, an association mapping panel of 230 winter wheat cultivars and breeding lines from Northern and Central Europe was employed. Genotyping with the Illumina ® iSelect ® 25 K Infinium ® SNP genotyping array yielded 8,812 polymorphic markers. Structure analysis revealed two subpopulations with 92 Austrian breeding lines and cultivars separated from the other 138 genotypes. A genome-wide association study for adult plant stripe rust resistance identified 12 SNP markers on six wheat chromosomes which showed consistent effects over several testing environments. Among these, two marker loci on chromosomes 2BS ( RAC875_c1226_652 ) and 6AL ( Tdurum_contig29607_413 ) were found highly predictive in three independent validation populations of 1065, 1001, and 175 breeding lines. Lines showing the resistant haplotype at both loci were found nearly free of stipe rust symptoms. Using linear models with these markers as fixed effects, we could increase predictive ability in the three populations by 0.13–0.46 compared to a standard genomic best linear unbiased prediction approach. The obtained results facilitate an efficient selection for stripe rust resistance against the current pathogen population in the Northern and Central European winter wheat gene pool.
Operando nanoscale imaging reveals Fe doping of Ni oxide enhancing oxygen evolution reaction via fragmentation and formation of dual active sites
Authors: Liang Yunchang , Parreiras Sofia , Lee Seunghwa , Banjac Karla , Boureau Victor , Gallego José María , Hu Xile , Écija David , Lingenfelder Magalí 
Published: March 2022

Abstract

Efficient catalytic water splitting demands advanced catalysts to improve the slow kinetics of the oxygen evolution reaction (OER). Earth-abundant transition metal oxides show promising OER activity in alkaline media. However, most experimental information available is either from post-mortem studies or in-situ space-averaged X-ray techniques in the micrometer range. Therefore, the composition of the active centers under operando conditions is still under debate. In this work, we combine nanoscopic and spectroscopic measurements on the hydroxylation of molecular beam epitaxy (MBE)-prepared Ni and NiFe oxides nanoislands with operando local investigations of Ni and NiFe hydroxide electrocatalysts under OER conditions to reveal the nature of active centers of Ni and Fe-doped Ni oxides in 2D OER catalysts. Our results reveal that Fe doping increases the active surface area by island fragmentation and boosts the intrinsic activity by creating optimized active centers consisting of both Ni and Fe atoms. In addition, our findings show that operando characterization at the nanoscale is crucial to reveal the dynamic nature of the interface of 2D catalysts under reaction conditions.
SENTIDOS E SIGNIFICADOS ATRIBUÍDOS À PRESENÇA DO ESTAGIÁRIO DE PEDAGOGIA EM UMA SALA DE AULA INCLUSIVA DA REDE PÚBLICA
Authors: Mariano Ana Paula , Lessa Angela B. Cavenagh Themudo , Oliveira Grassinete Carioca de Albuquerque 
Published: March 2022

Este artigo, apoiado na perspectiva teórica sócio-histórica-cultural, promove uma discussão crítica acerca da inclusão de crianças com deficiência sob a perspectiva de docentes e estagiários do curso de Pedagogia. Objetiva desencadear reflexão sobre os sentidos e significados atribuídos a presença de um estagiário em uma sala de aula inclusiva. Utiliza exemplos selecionados de um corpus de pesquisa coletado em uma escola municipal de São Paulo, extraídos de uma dissertação de mestrado de 2019, que subsidiarão a discussão apoiada em Vygotsky ([1924] 1997; 1930; [1930] 2008; [1930] 2010; [1934] 2001). Os resultados apontam para uma revisão e reflexão crítica sobre o papel do estagiário em uma sala de aula inclusiva.
Diseño y Validación de un instrumento para evaluar competencia digital en estudiantes de primer año de las carreras de educación de tres universidades públicas de Chile
Authors: Silva-Quiroz Juan-Eusebio , Abricot-Marchant Nicole , Aranda-Faúndez Gonzalo , Rioseco-País Marcelo 
Published: March 2022

La competencia digital (CD) es fundamental para el desarrollo personal y académico de los estudiantes universitarios actuales. Evaluar el nivel de la CD en los primeros años de formación universitaria de los estudiantes de carreras de pedagogía es relevante para introducir mejoras en las carreras del área de educación. El Objetivo general de este trabajo, fue diseñar y validar un instrumento de evaluación COMPDIG-PED (Competencia digital en pedagogía), en un contexto de carreras del área de la educación de tres universidades públicas chilenas. En este artículo se presenta el diseño y validación de un instrumento de evaluación de la CD denominado COMPDIG-PED, basado en el marco DIGCOMP (Digital Competence) elaborado por la comunidad económica europea. Se establece su fiabilidad y validez de constructo, junto con los análisis estadísticos correspondientes, habiendo trabajado con juicio de expertos. La muestra estuvo constituida por 841 estudiantes. Los resultados muestran que COMPDIG-PED es un instrumento que cumple con los estándares para medir la CD de los estudiantes de primer año de las carreras de pedagogía en particular y de cualquier carrera universitaria en general.
Estimation methods to define reference evapotranspiration: a comparative perspective
Authors: Pinos Juan 
Published: March 2022

Abstract
Evapotranspiration is a key variable for hydrologic, climatic, agricultural, and environmental studies. Given the non-availability of economically and technically easy to implement direct measurement methods, evapotranspiration is estimated primarily through the application of empirical and regression models, and machine learning algorithms that incorporate conventional meteorological variables. While the FAO-56 Penman-Monteith equation worldwide has been recognized as the most accurate equation to estimate the reference evapotranspiration (ETo), the number of required climatic variables makes its application questionable for regions with limited ground-based climate data. This note provides a summary of empirical and semi-empirical equations linked to its data requirement and the problems associated with these models (transferability and data quality), an overview of regression models, the potential of machine learning algorithms in regression tasks, trends of reference evapotranspiration studies, and some recommendations of the topics future research should address that would lead to a further improvement of the performance and generalization of the available models. The terminology used in this note is consistent in both the theoretical and practical field of evapotranspiration, which is often dispersed in the academic literature. The goal of this note is to provide some perspective to stimulate discussion.