bluuebunny/crossref_metadata_2025_split huggingface dataset:
How Did China's Digital Rural Policy Rapidly Diffuse? An Event History Analysis
Authors: xiong chunlin , hu siqi , jiang Duo 
Published: January 2025

Abstract

How to achieve the rapid dissemination of digital rural policies has become an important issue for bridging the digital divide in rural areas and realizing the modernization of agriculture and rural regions. This study adopts a dual perspective of policy adoption and internalization, constructing an analytical framework that includes internal demand pull, local government fiscal capacity to support rural development, central authority push, and intergovernmental competition as internal and external driving factors. It collects and analyzes data on the diffusion of digital rural policies among provincial governments in China from 2018 to 2022, employing event history analysis to explore the influencing factors and potential mechanisms behind the dissemination of digital rural policies in different regions of China.The findings reveal that, first, the diffusion of digital rural policies among provincial governments in China exhibits different outcomes at various stages. During the adoption stage, the diffusion outcomes show convergent characteristics, while in the internalization stage, they display divergent and diverse features. Second, in the process of digital rural policy diffusion, only a small number of provinces synchronized internalization with adoption, whereas most provinces adopted a strategy of first adopting and then internalizing.Third, the driving logic behind local governments' adoption and internalization of digital rural policies shares both commonalities and differences. The common driving forces include the intrinsic motivation brought about by population scale, the vertical authority push from the central government, and the horizontal learning and implicit competitive pressures from peer governments.This indicates that the diffusion of digital rural policies at the provincial level in China is driven by pull mechanisms, coercive mechanisms, learning mechanisms, and competitive mechanisms, with local governments employing competitive strategies in the adoption and internalization of digital rural policies.The differences manifest in that local government capacity is a significant influencing factor for adoption but does not notably impact the sustained advancement of policies, highlighting the important tendency to mobilize various stakeholders and activate resources during the internalization stage. These findings illuminate how digital rural policies can rapidly diffuse in China and provide empirical insights for developing countries to accelerate the dissemination of rural digital policies.
Portrait of Graduate Theology In Indonesia: The Conflict Between God's Needs and Self-Life Needs
Authors: GP Harianto , Ming David , Slamet Triadi  , Novita Loma Sahertian  
Published: January 2025

The background study explores Portrait of Graduate Theology in Indonesia as it relates to the dilemma between serving God and your own needs, particularly in light of the increasing number of theology graduates and their employment challenges. The increase in the number of theology majors by 13% raises concerns, given that 42% of these graduates face unemployment. This situation highlights a significant gap in theological education, where the quality may not meet the job market demands. The objective research aims to understand how theological education can better prepare students for spiritual service and practical life needs. This research employs a literature review approach, examining existing theoretical frameworks and empirical data on the social dynamics surrounding theology graduates. It examines various customs, norms, and societal expectations influencing these graduates' educational experiences and career paths. The increasing number of theology graduates juxtaposed with high unemployment presents a critical challenge for theological education. By adopting holistic approaches integrating spiritual formation with practical applications, institutions can better prepare students to navigate their dual roles in serving God and fulfilling personal life needs. This alignment could ultimately reduce unemployment rates among theology graduates while enriching their contributions to society.
Pro-renin Receptor (PRR) as a Target to Reduce Non-alcoholic Fatty Liver Disease Post Liver Transplantation
Authors: Khodashahi Rozita , Ferns Gordon A. , Aliakbarian Mohsen , Arjmand Mohammad-Hassan 
Published: January 2025

Abstract:
Nonalcoholic fatty liver disease (NAFLD) is one of the main causes of chronic
liver disorders following liver transplantation. The prorenin receptor (PRR) plays a
role in glucose and lipid metabolism, and the hepatic dysregulation of PRR is associated
with the upregulation of several molecular pathways, such as the mammalian target of rapamycin
(mTOR) and Peroxisome proliferator-activated receptor (PPAR) that promotes
hepatic lipogenesis and leads to lipid accumulation in hepatocytes by upregulation of lipogenic
genes. PRR inhibition leads to a reduction in the hepatic expression of sortilin-1
and low-density lipoprotein receptor (LDLR) levels and down-regulation of pyruvate dehydrogenase
(PDH) and acetyl-CoA carboxylase (ACC) and reduces fatty acids synthesis
in hepatocytes. In addition, β-oxidation regulatory genes are upregulated by PRR inhibition
to attenuate liver lipid content and liver steatosis. This evidence suggests that
targeting the dysregulated hepatic PRR may be effective in reducing liver steatosis postliver
transplantation.
Probing the Molecular Mechanisms of Kratom's Antipsychotic Effects through a Multi-modal Computational Approach
Authors: Wimbarti Supra , Tallei Trina Ekawati , Kairupan B.H. Ralph , Kapantow Nova Hellen , Ekatanti Dewi , Fatriani Rizka , Kusuma Wisnu Ananta , - Fatimawali , Celik Ismail 
Published: January 2025

Background:
Psychosis, marked by detachment from reality, includes symptoms like hallucinations
and delusions. Traditional herbal remedies like kratom are gaining attention for psychiatric conditions.
This was aimed at comprehending the molecular mechanisms of Kratom's antipsychotic effects utilizing a
multi-modal computational approach.

Materials and Methods:
This study employed network pharmacology followed by molecular docking and
molecular dynamics simulation study to investigate the potential antipsychotic properties of kratom compounds
by identifying their key molecular targets and interactions.

Results:
Compounds present in kratom interact with a variety of receptors and proteins that play a pivotal role
in neurotransmission, neurodevelopment, and cellular signaling. These interactions, particularly with dopamine
and serotonin receptors, various proteins, and pathways, suggest a complex influence on psychiatric
conditions. Both mitragynine and zotepine (an atypical antipsychotic drug) display significant binding affinities
for 5HTR2A receptors, suggesting their potential for modulating related physiological pathways. Mitragynine
displayed higher flexibility in binding compared to zotepine, which showed a more stable interaction.
Hydrogen bond analysis revealed a more variable interaction profile for mitragynine than zotepine.

Conclusion:
The research findings suggest that the interaction between kratom compounds and essential brain
receptors could influence psychiatric conditions. Notably, both mitragynine (a key kratom component) and
zotepine (an antipsychotic) bind to the 5HTR2A receptor, suggesting the potential for kratom to modulate
similar pathways. Interestingly, mitragynine's flexible binding mode compared to zotepine might indicate a
more diverse range of effects. Overall, the findings suggest complex interactions between kratom and the
brain's signaling system, warranting further investigation into its potential therapeutic effects.
PPA1, TRIM68 and FBXO46: Potential Therapeutic Targets for Triple Negative Breast Cancer
Authors: Haider Fatima , Syed Nida , Jaffari Syeda Abiha Zehra , Syed Basir , Ahmed Aftab , Zarina Shamshad , Hashim Zehra 
Published: January 2025

Background:
Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer
with a high recurrence rate. A new therapeutic intervention is urgently needed to combat this
lethal subtype. The identification of biomarkers is also crucial for improving outcomes in TNBC.

Method:
The cell cytotoxicity of ML364 (2-(4-Methylphenylsulfonamido)-N-(4-phenylthiazol-
2-yl)-4-(trifluoromethyl)benzamide) was measured at different concentrations of ML364 in
TNBC-treated and untreated cells. The 2DE and LC-MS/MS analysis were used for protein identification
of differentially expressed proteins. Furthermore, the quantitation of gene expression was
demonstrated using RT-qPCR. TIMER, HPA, and UALCAN databases were utilized for further
analysis.

Results:
Differentially expressed proteins and genes after ML364 treatment in TNBC were found
to be linked with the USP2 (ubiquitin specific peptidase 2)-mediated pathway. Our results demonstrate
that differentially identified proteins, including PPA1, TRIM68, and FBXO46, could be a potential
prognostic biomarker for TNBC. Further analysis through the UALCAN and HPA
databasess showsthe high expression of these proteins in primary breast tumors, which is in contrast
to normal. The induction of ML364 significantly reduced the expression of PPA1, TRIM68,
and FBXO46 proteins and induced cell cytotoxicity in TNBC cells.

Conclusion:
This study provides an understanding of the USP2-mediated signaling pathway in
TNBC, emphasizing the role of USP2 and its substrates with apoptotic genes. Our results offer insight
into the USP2-mediated cellular mechanism after ML364 treatment in TNBC that could be a
potential therapeutic candidate.
Neurotrophins as Potential Gene Therapy Targets for Huntington's Disease
Authors: Roy Sagor Kumar , Barman Ashima , Sarkar Kumary Labone , Richard Seidu A , Lacmane Bijal Arvinkumar 
Published: January 2025

Abstract:
“Huntington's disease” (HD) is an autosomal dominant hereditary neurodegenerative
disease characterized by defects in efferent striatal neurons, cortical neurons, and the basal ganglia.
The pathogenesis of HD is still unclear, and there is currently no curative therapy for this disorder.
This review emphasizes the potential beneficial effects of various neurotrophic factors in
HD. PubMed, Web of Science, Embase, and google scholar databases were used to search for all
studies on the efficacy of neurotrophic factors in HD. Several gene therapy strategies have been
employed to treat HD, including gene therapy with a variety of neuroprotective factors. Moreover,
a wide variability of gene therapy approaches such as a neurotrophin, has shown promising results
for both prevention and neuroprotection in HD, which may be due to their potential to prevent neuronal
cell death or decrease neurodegeneration, thereby promoting the growth of innovative axons,
dendrites, and synapses leading to improvement of HD. Neurotrophic factors may be suitable
as neuroprotective therapy agents in HD. Therefore, substantial research on gene therapy should
be conducted to provide better treatment options for HD in the future.
Impact of Copper(II) and Silver(I) Complexes Containing 1,10-Phenanthroline-5,6-dione on Cellular and Virulence Aspects of Scedosporium apiospermum
Authors: Pereira de Mello Thaís , A. Silva Bianca , Lione Viviane , Devereux Michael , McCann Malachy , Branquinha Marta Helena , Luis Souza dos Santos André 
Published: January 2025

Background:
Scedosporium apiospermum is a multidrug-resistant filamentous fungus
that causes localized and disseminated diseases. Our group has previously described that metalbased
complexes containing copper(II) or silver(I) ions complexed with 1,10-phenanthroline-5,6-
dione (phendione) inhibited the viability of S. apiospermum conidial cells.

Objective:
The effects of these promising complexes, [Cu(phendione)3](ClO4)2.4H2O (Cuphendione)
and [Ag(phendione)2]ClO4 (Ag-phendione), on vital biological processes, production of
key virulence attributes and interaction events of S. apiospermum were investigated using a comprehensive
multimodal approach.

Results:
The results demonstrated that both Cu-phendione and Ag-phendione effectively inhibited
the viability of S. apiospermum mycelial cells in a dose-dependent manner. Furthermore, these test
complexes, at varying concentrations, inhibited the transition of S. apiospermum conidia into hyphae.
Scanning electron microscopy revealed significant structural alterations in the fungal cells,
including changes to surface sculpturing and overall morphological architecture, following treatment
with the complexes. A marked reduction in the expression of key surface molecules, such as
mannose/glucose-rich glycoconjugates, fibronectin-binding proteins, and the well-known adhesin
peptidorhamnomannan further supported these ultrastructural changes. The treatment also impaired
adhesive interactions, reducing the fungus's ability to form biofilms on polystyrene surfaces and
diminishing its interaction with macrophages, lung epithelial cells, and fibroblasts. Notably, treatment
of infected macrophages with the complexes led to a significant reduction in the number of
intracellular fungal cells.

Conclusion:
The results provide information about the effects of silver- and copper-phendione
complexes on cellular and virulence aspects of the emerging fungus S. apiospermum.

conclusion:
Collectively, the results presented herein add information about the promising effects of silver- and copper-phendione complexes on cellular and virulence aspects of the emergent, multidrug-resistant fungus S. apiospermum
Updates on Recent Advances in the Therapy of Adult Psoriatic Disease
Authors: Kharouf Fadi , Gladman Dafna D 
Published: January 2025

Abstract:
Psoriatic arthritis (PsA) is a heterogeneous inflammatory disease with various joint and
skin manifestations and multiple associated comorbidities. The management of PsA is important
not only in controlling disease activity and preventing subsequent damage but also in improving
the quality of life and reducing mortality. Over the years, numerous drugs have been introduced into
the therapeutic armamentarium of the disease. While non-steroidal anti-inflammatory drugs (NSAIDs)
and conventional synthetic disease-modifying anti-rheumatic drugs (DMARDs) have contributed
to management, it was not until the advent of biologics (and later on targeted synthetic
DMARDs) that therapy was revolutionized, with the achievement of significantly better clinical
and radiographic outcomes. Several drugs and treatment approaches are currently being tested in
clinical trials at different phases. Despite all the success, there are still various challenges and unmet
needs in the field of PsA, reflected by difficult-to-treat disease course, secondary failure of
therapy, and lack of consensus on accepted treatment withdrawal protocols, among others.
In this review, we have discussed the most recent advances in the therapy of psoriatic disease,
with a particular focus on phase III studies completed (or ongoing) since 2020. We also mentioned
the challenges and unmet needs in our clinical practice, which we expect current and future
research to provide answers to.
Detection of Anti-drug Antibodies (ADAs) to an Antibody-drug Conjugate (ADC) PYX-201 in Human Plasma Using a Novel Electrochemiluminescence
(ECL) Immunoassay
Authors: Yin Feng , Adhikari Diana , Lara Rojo Ana , Kelly Laura , Ibdah Areeg , Orbegoso Ivan , Eak Gwen , Merriman Chengfeng , Feng Jianwen , Trexler Kristen , Turner Dunford Ann , Ospina Roxana , Dyszel Mike , Harriman Shawn , Pinkas Jan 
Published: January 2025

Background:
PYX-201 is an Antibody-Drug Conjugate (ADC) composed of a fully human
IgG1 antibody, a cleavable linker mcValCitPABC, and toxic auristatin payloads Aur0101, with
a drug antibody ratio (DAR) of approximately 4. PYX-201 is a promising candidate for oncology
treatment because it targets the extra domain B splice variant of fibronectin (EDB + FN), which is
expressed at low levels in normal adult tissues while at moderate or high levels in various human
solid tumors.

Methods:
An electrochemiluminescence (ECL) immunoassay was developed and validated for the
detection (screening, confirmatory, and titration) of antibodies to an ADC PYX-201 in human plasma.
Anti-PYX-201 antibodies were captured by biotinylated PYX-201 (Bio-PYX-201) and detected
by ruthenylated PYX-201 (Ru-PYX-201) on a Meso Sector imager S 600 or 6000 reader.

Results:
The screening cut-point factor (SCPF), confirmatory cut-point (CCP), and titration cut-point
factor (TCPF) were found to be 1.11, 20.7%, and 1.23, respectively. Sensitivity was determined to be
2.25 ng/mL in the screening assay and 5.34 ng/mL in the confirmatory assay for anti-PYX-201 antibodies.
Sensitivity was determined to be 7.70 ng/mL in the confirmatory assay for anti-PYX-201
monoclonal antibody (mAb) antibodies. The positive controls (PCs) were set at the following levels:
low positive control (LPC) at 14.0 ng/mL, medium positive control (MPC) at 100 ng/mL, and high
positive control (HPC) at 5,000 ng/mL. The drug tolerance was up to 200 μg/mL at the HPC level,
up to 100 μg/mL at the MPC level, and 0 μg/mL at the LPC level. The intra-assay percent coefficient
of variation (%CV) was ≤ 4.5% for PCs in the screening assay and ≤ 11.5% for PCs in the confirmatory
assay. The inter-assay %CV was ≤ 13.6% for PCs in the screening assay and ≤ 19.2% for PCs in
the confirmatory assay. No hook effect, hemolysis effect, or lipemia effect was found in this ADA
method. Anti-PYX-201 antibodies were found stable in human plasma for at least 24 hours at room
temperature or after six freeze/thaw cycles.

Conclusion:
Anti-PYX-201 ADA bioanalytical method validation was reported for the first time in
any biological matrix. This ADA method has been successfully applied to human sample analysis to
support a clinical study.
Investigating the Prevalence of Suicidal Risk and Associated Risk Factors Among University Students in Southeastern Iran: A Cross-Sectional Study
Authors: Mehni Esmaeil Barkhori , Raesi Rasoul , Ahmadidarrehsima Sudabeh , Domari Asma Amiri , Amiri Mohammad Moqaddasi , Ardaneh Mahdieh , Hushmandi Kiavash , daneshi salman 
Published: January 2025

Background:
Suicide is a significant health issue among young people
globally. According to available statistics and research, it ranks as the third leading
cause of death for individuals aged 15-24 and the second leading cause of death
among students.

Aim:
This study was conducted to investigate the risk of suicide and its related
factors among students at Jiroft University of Medical Sciences.

Methods:
The descriptive study of the current analysis is a cross-sectional type that
was conducted using a multi-stage sampling method on 128 students at Jiroft
University of Medical Sciences in 2022. Data were collected using the Beck Scale for
Suicide Ideation (BSSI) and analyzed with statistical tests of analysis of variance,
Pearson correlation, and multiple regression analysis in SPSS-24 statistical software.

Results:
The average age of students was 22.14 ± 2.96 years. Seventeen individuals
(13.3%) had a history of suicide attempts. The average suicide risk score was 6.18 ±
5.61, indicating a high risk of suicide among students. The suicide risk score differed
between individuals with strong religious beliefs and those with little belief
(p=0.025). The average score for suicidal risks was lower in individuals with
complete religious beliefs.

Conclusion:
The study shows that students are at a high risk of suicide. This
highlights the need to prioritize mental health, implement effective suicide prevention
strategies, and personalize interventions to reduce the risk of suicide among medical
students.
Characterization and Identification of Oleaginous Microalgae Isolated from Fresh Water for Biodiesel Production
Authors: Jaithaliya Radhika , Jain Divya , Chakraborty Dipjyoti , Dutt Kakoli 
Published: January 2025

Introduction:
Developing sustainable biodiesel production relies on investigating local
microalgal populations to detect neutral lipid accumulation via high throughput screening. Method:
This study evaluates the efficacy of using various isolation strategies for maximizing microalgal
strain collection from low-abundance water samples.

Result:
The study resulted in the isolation of twenty-five algal strains, of which 3 oleaginous strains
were identified as Chlorococcum aquaticum BB607, Chlorococcum sp. BB601 and Spongiosarcinopsis
limneus BG607 were selected. Isolate C. aquaticum BB607 exhibited substantial lipid content
of 456.45 ± 2.40 μg/mL, lipid productivity of 38.04 ± 0.20 μg/mL/day, biomass yield of 4.23 ± 0.06
mg/mL and maximum percentage C16-C18 fatty acid profile compared to the other two isolates.

Conclusion:
Further, the FAMEs produced from this isolate exhibited high CN (60.384), low iodine
value (97.33 g I2/100 g) and negative cold filter plugging point (- 2.28°C). This demonstrates the
potential of C. aquaticum strain BB607 as a feedstock biodiesel production.
Cause Analysis of Rotork Electric Actuator Phase Loss Fault Mode
Authors: Li Jian , Hu Xiaoguang , Gao Zhigang , Niu Pingjuan , Guo Jingying , Lin Sixuan , Yan Zhike , Ren Hao , Zhang Hai , Liu Hairui , Song Yinglu , Song Shen , Zhu Leilei , Yang Chao 
Published: January 2025

Background:
The stable, safe, and reliable operation of the electric actuator valve system is
crucial in industrial production. However, its faults can lead to production interruptions, equipment
damage, and increased costs. Therefore, detecting and analyzing the causes of fault modes is crucial for
exploring methods to reduce their occurrence and ensuring the normal operation of electric actuators.

Methods:
Taking the IQ20-F14-A electric actuator from Rotork, UK, as a reference, this paper
analyzes the causes of the phase loss fault mode from three aspects: software, circuitry, and external
conditions. By analyzing and experimentally testing the power supply information extraction
circuit, identifying the extraction signal related to phase information, and further comprehensively
listing the factors that lead to phase loss faults, the scope is narrowed down through analysis and
practical verification.

Results:
After investigating potential factors related to component design, circuit design, and connection
issues within the circuit, causing a decay of a certain phase voltage on the power line to
below the threshold leads to the optocoupler and information-extracting transistors entering a cutoff
state, resulting in a phase loss alarm. Additionally, the continuous superposition of typical interference
pulse trains on the input voltage for 9 seconds or more also triggers a phase loss alarm.

Conclusion:
The fault mode mainly revolves around software, the decay of external supply phase
voltage, and the interference of external phase voltage.