bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of IL‐8, osteonectin, and myonectin mRNAs by intermittent hypoxia via OCT1‐ and NRF2‐mediated mechanisms in skeletal muscle cells
Authors: Takasawa Shin , Shobatake Ryogo , Itaya‐Hironaka Asako , Makino Mai , Uchiyama Tomoko , Sakuramoto‐Tsuchida Sumiyo , Takeda Yoshinori , Ota Hiroyo , Yamauchi Akiyo 
Published: December 2022

AbstractSleep apnoea syndrome is characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]) and is a risk factor for insulin resistance/Type 2 diabetes. The induction of insulin resistance in skeletal muscle is a key phenomenon to develop diabetes. However, the mechanisms linking IH stress and insulin resistance remain elusive. We exposed human RD and mouse C2C12 muscle cells to normoxia or IH and measured their mRNA levels by real‐time RT‐PCR. We found that IH significantly increased the mRNA and protein levels of muscle‐derived insulin resistance‐factors (myokines) such as IL‐8, osteonectin (ON), and myonectin (MN) in muscle cells. We further analysed the IH‐induced expression mechanisms of IL‐8, ON, and MN genes in muscle cells. Deletion analyses of the human myokine promoter(s) revealed that the regions −152 to −151 in IL‐8, −105 to −99 in ON, and − 3741 to −3738 in MN promoters were responsible for the activation by IH in RD cells. The promoters contain consensus transcription factor binding sequences for OCT1 in IL‐8 and MN promoters, and for NRF2 in ON promoter, respectively. The introduction of siRNA for OCT1 abolished the IH‐induced expression(s) of IL‐8 and MN and siRNA for NRF2 abolished the IH‐induced expression of ON.
Pontine lesion in hyperglycemic crises: Relevance to osmotic demyelination syndrome and posterior reversible encephalopathy syndrome
Authors: Murao Satoshi , Kiuchi Takaaki , Hasegawa Momoka , Yoshikawa Ritsuko 
Published: December 2022

AbstractWe herein describe a case of type 1 diabetes that presented with a pontine lesion during two hyperglycemic crises accompanied by marked fluctuations in serum osmotic pressure and blood pressure. Magnetic resonance imaging showed swollen pons with osmotic demyelination syndrome characteristics accompanying cytotoxic edema at the first crisis. The involvement of vasogenic edema was also assumed in the second crisis. Neurological symptoms were milder than magnetic resonance imaging findings. The patient recovered after 7 days without sequelae in both crises. Based on these findings, a pontine lesion needs to be considered in patients with poorly controlled diabetes showing rapid metabolic and blood pressure changes, as observed in hyperglycemic crises. Cytotoxic edema leading to osmotic demyelination syndrome and vasogenic edema caused by vascular endothelial cell damage might both be involved in the pathogenesis of a pontine lesion.
XX/XY sex chromosomes in a blind lizard (Dibamidae): Towards understanding the evolution of sex determination in squamates
Authors: Rovatsos Michail , Galoyan Eduard , Spangenberg Victor , Vassilieva Anna , Kratochvíl Lukáš 
Published: December 2022

AbstractThe reconstruction of the evolutionary history of sex determination in squamate reptiles (lizards and snakes) is complicated by missing data in many lineages, erroneous reports, and often questionable inferences on state homology. Therefore, despite the large effort, the reconstruction of the ancestral sex determination in squamate reptiles is still controversial. With the hope to shed light on this problem, we aspired to identify the sex chromosome gene content in Dibamus deharvengi, the representative of the family Dibamidae, the putative sister clade to all other squamates. Our analyses revealed XX/XY sex‐determination system in D. deharvengi: the X chromosome contains genes with homologues scattered across chicken chromosomes 8, 12, 13, 18, 30, and 33, and the Y chromosome seems to largely degenerate. To the best of our knowledge, this combination has never been reported to form sex chromosomes in any amniote lineage. It suggests that the sex chromosomes can represent an apomorphy of a clade including D. deharvengi. Our findings cover an important gap in the knowledge of sex determination in reptiles and further support multiple independent origins of sex chromosomes in this group.
A bibliometric and visualized analysis of liver fibrosis from 2002 to 2022
Authors: Zhao Qianqian , Liang Luhua , Zhai Fei , Ling Guixia , Xiang Rongwu , Jiang Xiwei 
Published: December 2022

AbstractFibrosis of the liver is a degenerative alteration that occurs in the majority of chronic liver disorders. Further progression can lead to cirrhosis, liver failure, and hepatocellular carcinoma, which can seriously affect the health and lives of patients. The field of liver fibrosis research has flourished in the last 20 years, with approximately 9000 articles retrieved from the Web of Science Core Collection database alone. In order to identify future research hotspots and potential paths in a thorough and scientifically reliable manner, it is important to organize and visualize the research on this topic from a holistic and very general perspective. This study used bibliometric analysis with CiteSpace and VOSviewer software to provide a quantitative analysis, hotspot mining, and commentary of articles published in the field of liver fibrosis over the last 20 years. This bibliometric analysis contains a total of 8994 articles with 45667 authors from 6872 institutions in 97 countries, published in 1371 journals and citing 156 309 references. The literature volume has steadily increased over the last 20 years. Research has focused on gastroenterology and hepatology, pharmacology and pharmacy, and medicine, research, and experimental areas. We found that the pathological mechanisms, diagnostic and quantitative methods, etiology, and antifibrotic strategies constitute the knowledge structure of liver fibrosis. Finding mechanisms for liver fibrosis regression, identifying precise noninvasive diagnostic and prognostic biomarkers, and creating efficient liver fibrosis patient treatments are the main goals of current research.
The Undisciplined Youth and a Moral Panic in Independent India, Circa 1947‐1964
Authors: Wilkinson Tom 
Published: December 2022

AbstractThe undisciplined youth is one figure that is key to understanding the 1950s and 1960s in India. Politicians, officials, academics, youth leaders, and journalists developed and spread a discourse that imagined the collective behaviour of Indian youths as falling well below adult expectations of them in independent India. The imagery of the youth lacking in discipline was tied up with cycles of student unrest and the idea that the methods of protest used during the pre‐independence period had wrongly continued into the post‐independence period, but this discursive formation was often extended to include all Indian youths and it became translated into a long‐term anxiety about the future of the newly established nation‐state. These tropes about the undisciplined Indian youth became a symbol of the country's unresolved future. Unless the crisis of youth could be remedied, the narrative went, then the potentiality of Indian independence and its first generation of citizens could never be realised. This discourse took on a novel and distinctive shape during the initial years following Indian independence in 1947, it crystallised during the early 1950s, and there was a continued build‐up of public concern that lasted throughout the 1960s.
Melatonin ameliorates myocardial infarction in obese diabetic individuals: The possible involvement of macrophage apoptotic factors
Authors: Maity Juin , Dey Tiyasa , Banerjee Adrita , Chattopadhyay Aindrila , Das Asish R. , Bandyopadhyay Debasish 
Published: December 2022

AbstractIn recent days, the hike in obesity‐mediated epidemics across the globe and the prevalence of obesity‐induced cardiovascular disease has become one of the chief grounds for morbidity and mortality. This epidemic‐driven detrimental events in the cardiac tissues start with the altered distribution and metabolism pattern of high‐density lipoprotein and low‐density lipoprotein (LDL) leading to cholesterol (oxidized LDL) deposition on the arterial wall and atherosclerotic plaque generation, followed by vascular spasms and infarction. Subsequently, obesity‐triggered metabolic malfunctions induce free radical generation which may further trigger pro‐inflammatory signaling and nuclear factor kappa‐light‐chain‐enhancer of activated B cells transcriptional factor, thus inducing interferon‐gamma, tumor necrosis factor‐alpha, and inducible nitric oxide synthase. This terrifying cardiomyopathy can be further aggravated in type 2 diabetes mellitus, thereby making obese diabetic patients prone toward the development of myocardial infarction (MI) or stroke in comparison to their nondiabetic counterparts. The accelerated oxidative stress and pro‐inflammatory response induced cardiomyocyte hypertrophy, followed by apoptosis in obese diabetic individuals, causing progression of athero‐thrombotic vascular disease. Being an efficient antioxidative and anti‐inflammatory indolamine, melatonin effectively inhibits lipid peroxidation, pro‐inflammatory reactions, thereby resolving free radical‐induced myocardial damages along with maintaining antioxidant reservoir to preserve cardiovascular integrity. Prolonged melatonin treatment maintains balanced body weight and serum total cholesterol concentration by inhibiting cholesterol synthesis and promoting cholesterol catabolism. Additionally, melatonin promotes macrophage polarization toward the anti‐inflammatory state, providing a proper shield during the recovery period. Therefore, the protective role of melatonin in maintaining the lipid metabolism homeostasis and blocking the atherosclerotic plaque rupture could be targeted as the possible therapeutic strategy for the management of obesity‐induced acute MI. This review aimed at orchestrating the efficacy of melatonin in ameliorating irrevocable oxidative cardiovascular damage induced by the obesity–diabetes correlation.
Abundant copathologies of polyglucosan bodies, frontotemporal lobar degeneration with TDP‐43 inclusions and ageing‐related tau astrogliopathy in a family with aGBE1mutation
Authors: Uemura Maiko T. , Suh Eun Ran , Robinson John L. , Brunden Kurt R. , Grossman Murray , Irwin David J. , Lee Virginia M.‐Y. , Trojanowski John Q. , Lee Edward B. , Van Deerlin Vivianna M. 
Published: December 2022

AbstractAimsAdult polyglucosan body disease (APBD) is a progressive neurogenetic disorder caused by 1,4‐alpha‐glucan branching enzyme 1 (GBE1) mutation with an accumulation of polyglucosan bodies (PBs) in the central and peripheral nervous systems as a pathological hallmark. Here, we report two siblings in a family with aGBE1mutation with prominent frontotemporal lobar degeneration with TAR DNA‐binding protein 43 (FTLD‐TDP) and ageing‐related tau astrogliopathy (ARTAG) copathologies with PBs in the central nervous system.MethodsWhole‐genome sequencing (WGS) followed by Sanger sequencing (SS) was performed on three affected and two unaffected siblings in a pedigree diagnosed with familial frontotemporal dementia. Out of the affected siblings, autopsies were conducted on two cases, and brain samples were used for biochemical and histological analyses. Brain sections were stained with haematoxylin and eosin and immunostained with antibodies against ubiquitin, tau, amyloid β, α‐synuclein, TDP‐43 and fused in sarcoma (FUS).ResultsA novel single nucleotide deletion inGBE1, c.1280delG, was identified, which is predicted to result in a reading frameshift, p.Gly427Glufs*9. This variant segregated with disease in the family, is absent from population databases and is predicted to cause loss of function, a known genetic mechanism for APBD. The affected siblings showed a greater than 50% decrease in GBE protein levels. Immunohistochemical analysis revealed widespread FTLD‐TDP (type A) and ARTAG pathologies as well as PBs in the brains of two affected siblings for whom an autopsy was performed.ConclusionsThis is the first report of a family with several individuals with a FTD clinical phenotype and underlying copathologies of APBD, FTLD‐TDP and ARTAG with a segregatingGBE1loss‐of‐function mutation in affected siblings. The finding of copathologies of APBD and FTLD‐TDP suggests these processes may share a disease mechanism resulting from thisGBE1mutation.
Global dissection of the recombination landscape in soybean using a high‐density 600K SoySNP array
Authors: Ma Xin , Fan Lei , Zhang Zhifang , Yang Xia , Liu Yucheng , Ma Yanming , Pan Yi , Zhou Guoan , Zhang Min , Ning Hailong , Kong Fanjiang , Ma Junkui , Liu Shulin , Tian Zhixi 
Published: December 2022

SummaryRecombination is crucial for crop breeding because it can break linkage drag and generate novel allele combinations. However, the high‐resolution recombination landscape and its driving forces in soybean are largely unknown. Here, we constructed eight recombinant inbred line (RIL) populations and genotyped individual lines using the high‐density 600K SoySNP array, which yielded a high‐resolution recombination map with 5636 recombination sites at a resolution of 1.37 kb. The recombination rate was negatively correlated with transposable element density and GC content but positively correlated with gene density. Interestingly, we found that meiotic recombination was enriched at the promoters of active genes. Further investigations revealed that chromatin accessibility and active epigenetic modifications promoted recombination. Our findings provide important insights into the control of homologous recombination and thus will increase our ability to accelerate soybean breeding by manipulating meiotic recombination rate.
Red‐light receptor phytochrome B inhibits BZR1‐NAC028‐CAD8B signaling to negatively regulate rice resistance to sheath blight
Authors: Yuan De Peng , Yang Shuo , Feng Lu , Chu Jin , Dong Hai , Sun Jian , Chen Huan , Li Zhuo , Yamamoto Naoki , Zheng Aiping , Li Shuang , Yoon Hong Chan , Chen Jingsheng , Ma Dianrong , Xuan Yuan Hu 
Published: December 2022

AbstractPhytochrome (Phy)‐regulated light signalling plays important roles in plant growth, development, and stress responses. However, its function in rice defence against sheath blight disease (ShB) remains unclear. Here, we found that PhyB mutation or shade treatment promoted rice resistance to ShB, while resistance was reduced by PhyB overexpression. Further analysis showed that PhyB interacts with phytochrome‐interacting factor‐like 15 (PIL15), brassinazole resistant 1 (BZR1), and vascular plant one‐zinc‐finger 2 (VOZ2). Plants overexpressing PIL15 were more susceptible to ShB in contrast to bzr1‐D‐overexpressing plants compared with the wild‐type, suggesting that PhyB may inhibit BZR1 to negatively regulate rice resistance to ShB. Although BZR1 is known to regulate brassinosteroid (BR) signalling, the observation that BR signalling negatively regulated resistance to ShB indicated an independent role for BZR1 in controlling rice resistance. It was also found that the BZR1 ligand NAC028 positively regulated resistance to ShB. RNA sequencing showed that cinnamyl alcohol dehydrogenase 8B (CAD8B), involved in lignin biosynthesis was upregulated in both bzr1‐D‐ and NAC028‐overexpressing plants compared with the wild‐type. Yeast‐one hybrid, ChIP, and transactivation assays demonstrated that BZR1 and NAC028 activate CAD8B directly. Taken together, the analyses demonstrated that PhyB‐mediated light signalling inhibits the BZR1‐NAC028‐CAD8B pathway to regulate rice resistance to ShB.
Genome‐wide identification and expression analysis of SlRR genes in response to abiotic stress in tomato
Authors: Liu H. , Chen R. , Li H. , Lin J. , Wang Y. , Han M. , Wang T. , Wang H. , Chen Q. , Chen F. , Chu P. , Liang C. , Ren C. , Zhang Y. , Yang F. , Sheng Y. , Wei J. , Wu X. , Yu G. 
Published: December 2022

Abstract
The cytokinin two‐component signal transduction system (TCS) is involved in many biological processes, including hormone signal transduction and plant growth regulation. Although cytokinin TCS has been well characterized in Arabidopsis thaliana, its role in tomato remains elusive.
In this study, we characterized the diversity and function of response regulator (RR) genes, a critical component of TCS, in tomato. In total, we identified 31 RR genes in the tomato genome. These SlRR genes were classified into three subgroups (type‐A, type‐B and type‐C).
Various stress‐responsive cis‐elements were present in the tomato RR gene promoters. Their expression responses under pesticide treatment were evaluated by transcriptome analysis. Their expression under heat, cold, ABA, salinity and NaHCO3 treatments was further investigated by qRT‐PCR and complemented with the available transcription data under these treatments. Specifically, SlRR13 expression was significantly upregulated under salinity, drought, cold and pesticide stress and was downregulated under ABA treatment. SlRR23 expression was induced under salt treatment, while the transcription level of SlRR1 was increased under cold and decreased under salt stress.
We also found that GATA transcription factors played a significant role in the regulation of SlRR genes. Based on our results, tomato SlRR genes are involved in responses to abiotic stress in tomato and could be implemented in molecular breeding approaches to increase resistance of tomato to environmental stresses.
Implications of product substitutability in a distribution channel
Authors: Zheng Quan , Hu Honggang , Pan Xiajun Amy 
Published: December 2022

This paper examines the effect of product substitutability on the equilibrium profits in a distribution channel of two symmetric manufacturers selling substitutable products to consumers through a nonexclusive retailer. Conventional wisdom suggests that when the products are more substitutable, the retailer will be better off while the manufacturers will be worse off, owing to the dominance of the competition effect of substitutability. In this work, we identify the hitherto neglected demand effect of substitutability and show that the relative strength between the competition and demand effects is the key to this result. As the products become more substitutable, the upstream competition does intensify, but vitally, the demand may expand. We show that the interests of the retailer and manufacturers can be aligned in terms of product substitutability, depending on both the demand and supply factors. Surprisingly, the conventional result can even be completely reversed such that the manufacturers benefit from a certain level of substitutability for some demand patterns. Assuming linear demand, we further isolate the important role of the bargaining power balance and the alternative of agency selling format in moderating the competition effect. For example, as the retailer gains more bargaining power, the (negative) demand effect can overshadow the (positive) competition effect such that a higher degree of substitutability can actually hurt the retailer. Our findings provide valuable implications for persuasive advertising and category management.
Environmental governance in China: The effects of policy clarity, career concerns, and new appointed officials on pollution control
Authors: Sun Jingran , Yue Linfeng , Li Xiangyu (Dale) , Dai Min 
Published: December 2022

AbstractIn the last three decades, China has taken actions to tackle its environmental issues while the tension between policymakers at the central level and decentralized implementation of such policies has been a major concern. This study investigates how policy clarity and high powered incentive system jointly affect organizational performance in the context of environmental governance in China. Utilizing city‐level data compiled with text data extracted from Report on the Work of the Government from 2004 to 2015 and Difference in Differences (DID) design, we find that the compliance of local officials to protect the environment as well as the actual environmental protection outcome significantly increased. Our study also finds evidence that newly‐appointed Party Secretaries at the city level are the main facilitators of enhancing environmental regulation policies. This research proposes a two by two typology based on the principal‐agent theory explaining how successful environmental governance within this period in China is realized and offers practical implications for those who seek to enhance the effectiveness of environmental governance.
Considerations for challenges with STEM standards alignment in the middle grades
Authors: Gonzales Jennifer A. , Ritter Kenley B. , Shelton Ryann N. 
Published: December 2022

AbstractResearch favors active learning through STEM disciplines to improve understanding; however, opportunities for well‐aligned STEM integration presented challenges for Northside Independent School District (NISD). First, state standards did not align across contents and grade levels. Second, the standardized tests created a lack of flexibility to adjust the pacing and sequencing of instruction. Third, there was a lack of resources that supported each of the content areas in an integrated and aligned STEM learning experience that benefited the individual content areas. To address these needs, Northside ISD used innovative practices to overcome their challenges with writing a middle school STEM curriculum. Despite the lack of resources available, the middle school curriculum and instruction team addressed these challenges by re‐sequencing standards, developing Design Challenge research opportunities, and identifying a unique STEM Context that could be carried throughout an instructional unit. This innovation addressed each core content area with fidelity and allowed for STEM alignment while also exposing students in the district to unique STEM opportunities.
Pepper stress‐associated protein 14 is a substrate of CaSnRK2.6 that positively modulates abscisic acid‐dependent osmotic stress responses
Authors: Bae Yeongil , Lim Chae Woo , Lee Sung Chul 
Published: December 2022

SUMMARYThe phytohormone abscisic acid (ABA) plays a prominent role in various abiotic stress responses of plants. In the ABA‐dependent osmotic stress response, SnRK2.6, one of the subclass III SnRK2 kinases, has been identified as playing a key role by phosphorylating and activating downstream genes. Although several modulatory proteins have been reported to be phosphorylated by SnRK2.6, the identities of the full spectrum of downstream targets have yet to be sufficiently established. In this study, we identified CaSAP14, a stress‐associated protein in pepper (Capsicum annuum), as a downstream target of CaSnRK2.6. We elucidated the physical interaction between SnRK2.6 and CaSAP14, both in vitro and in vivo, and accordingly identified a C‐terminal C2H2‐type zinc finger domain of CaSAP14 as being important for their interaction. CaSAP14‐silenced pepper plants showed dehydration‐ and high salt‐sensitive phenotypes, whereas overexpression of CaSAP14 in Arabidopsis conferred tolerance to dehydration, high salinity, and mannitol treatment, with plants showing ABA‐hypersensitive phenotypes. Furthermore, an in‐gel kinase assay revealed that CaSnRK2.6 phosphorylates CaSAP14 in response to exogenous ABA, dehydration, and high‐salinity stress. Collectively, these findings suggest that CaSAP14 is a direct substrate of CaSnRK2.6 and positively modulates dehydration‐ and high salinity‐induced osmotic stress responses.