bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Differential Gene Expression of the Aquatic Insect Protohermes costalis (Walker) (Megaloptera: Corydalidae) in Response to Cadmium Exposure
Authors: Wen Fasheng , Yang Jie , Huang Xingrui , Huang Xinglong 
Published: June 2022

Abstract
Heavy metal pollution in freshwater ecosystems is a serious threat to aquatic organisms. Species of Megaloptera are important predators of aquatic invertebrates and have been widely used as bioindicators in assessing the quality of freshwater ecosystems. In this study, we determined the differential gene expression profile of Protohermes costalis (Walker) (Megaloptera: Corydalidae) in response to cadmium (Cd) exposure by using transcriptome analysis. A total of 60,627 unigenes were obtained in the transcriptomes of 150 mg/liter (PL), 1,000 mg/liter (PH) CdCl2 treatment, and the no Cd control (PC). Differential expression gene (DEG) analysis by pairwise comparison identified 2,794 DEGs after filtering the noninsect genes and repetitive counts. 606 DEGs were shared in comparisons of PL versus PC and PH versus PC, with 165 DEGs consistently up-regulated and 441 down-regulated by both PL and PH. Six heat shock proteins (HSPs) in the HSP70 family were identified in P. costalis and PcosHSP68 was up-regulated by both PL and PH. Real-time quantitative polymerase chain reaction (RT-qPCR) confirmed that the expression levels of PcosHSP68 in PL and PH were higher than that of PC by 31 and 197%, respectively. These results showed that exposure to Cd altered the gene expression profiles of P. costalis and the transcriptome data presented in this study provide insight into future studying on molecular mechanisms of Cd toxicity to these insects.
Atrioventricular 2:1-conduction via an accessory pathway during left atrial flutter unmasking WPW syndrome: a case report
Authors: Huttelmaier Moritz Till , Herting Jonas , Fischer Thomas Horst 
Published: June 2022

Abstract

Background
Implantable cardioverter defibrillators (ICDs) are most effective in treating sudden cardiac death. However, accurate diagnostic workup of broad complex tachycardia is crucial to ensure correct indication for ICD treatment and to avoid unnecessary invasive treatment and device-associated morbidity.

Case summary
We present a case of atypical atrial flutter with 2:1 atrioventricular (AV) conduction via a left-posterior accessory pathway (AP), leading to the diagnosis of Wolff-Parkinson-White (WPW) syndrome. Upon admission, the 72-year-old patient showed a regular broad complex tachycardia with superior axis and positive concordance in precordial leads, suggestive of either ventricular tachycardia (VT), antidromic AV re-entrant tachycardia (AVRT), or supraventricular tachycardia with antegrade conduction via a left-posterior AP. Interrogation of the two-chamber ICD, which was very likely implanted unjustified in a peripheral clinic before, revealed atrial flutter with 2:1 AV conduction. Remarkably, after the restoration of sinus rhythm, no classic echocardiogram (ECG) criteria for preexcitation syndrome were detected. An invasive electrophysiological study proved the diagnosis of a bidirectionally conducting, left-posterior AP, which was successfully ablated.

Discussion
Differential diagnosis of broad complex tachycardia with superior axis and positive concordance of chest leads consists of i) VT with a left ventricular exit at the posterior mitral annulus, ii) antidromic AVRT involving a left-posterior AP, and iii) supraventricular tachycardia predominantly conducted via a left-posterior AP. The absence of classic ECG criteria for preexcitation syndrome does not rule out AP sufficiently, highlighting the importance of minimal surface-ECG preexcitation criteria. In the case of detection of minimal surface-ECG preexcitation criteria, administration of adenosine rules out or proves the existence of an AP noninvasively and cost-effectively.
Psychosocial and clinical characteristics of a patient with Takotsubo syndrome and her healthy monozygotic twin: a case report
Authors: Princip Mary , Langraf-Meister Rebecca E , Slavich George M , Pazhenkottil Aju P , Hackl-Zuccarella Claudia , Cammann Victoria L , Ghadri Jelena R , Templin Christian , von Känel Roland 
Published: June 2022

Abstract

Background
Takotsubo syndrome (TTS) is an acute heart failure syndrome characterized by transient left ventricular dysfunction, increased myocardial biomarkers, and electrocardiographic changes. Symptoms of TTS are similar to those of acute coronary syndromes, but there is often no significant coronary stenosis. Although emotional and physical stressors are often reported as having triggered TTS, the pathogenesis is largely unknown. To address this issue, we comprehensively characterized a monozygous pair of twin sisters, one of whom experienced TTS.

Case summary
The 60-year-old Caucasian monozygotic female twins with and without TTS were examined at the University Hospital Zurich in Switzerland. The twins completed questionnaires and clinical interviews assessing several sociopsychological factors. The twin sister with TTS exhibited higher levels of anxiety, vital exhaustion, social inhibition, and alexithymia, and lower levels of quality of and meaning in life. She was given the diagnoses of social phobia, adjustment disorder, specific anxiety disorder, obsessive–compulsive personality disorder, and an accentuated anxiety-avoidant personality disorder. Additionally, the twin with TTS experienced more—and also more severe—stressors involving life-threatening and dangerous situations over the life course.

Discussion
These monozygous female twins with and without TTS differed in several notable aspects of their psychological functioning, psychiatric status, personality, and lifetime stressor exposure. The results thus highlight several factors, besides genetic components, that may play an important role in the pathogenesis of TTS. Looking forward, larger studies using experimental and longitudinal designs are needed to elucidate the role that psychosocial factors play in TTS.
Histopathological findings of late-phase restenosis after directional coronary atherectomy with drug-coated balloon angioplasty: a case report
Authors: Yamamoto Hiroyuki , Emoto Takuo , Takeda Shintaro , Takaya Tomofumi 
Published: June 2022

Abstract

Background
Drug-coated balloon angioplasty after directional coronary atherectomy (DCA) allows for a stentless strategy providing good short-term outcomes; however, late-phase restenosis and its mechanism remain unclear. Moreover, histopathological evaluation for late-phase restenosis post-drug-coated balloon angioplasty after DCA has never been reported.

Case summary
We report the first case of late-phase restenosis post-drug-coated balloon angioplasty after DCA, wherein tissue analysis using intravascular coronary imaging and histopathology suggested neovascularization in newly developed neointimal proliferation. A 52-year-old man with a history of dyslipidaemia presented with exertional angina pectoris. He underwent percutaneous coronary intervention (PCI) with drug-coated balloon angioplasty after DCA for the proximal left anterior descending artery. Although coronary angiography after nine months revealed no restenosis, he experienced recurrent chest discomfort after 25 months. Coronary angiography confirmed late-phase restenosis, and intravascular ultrasound showed progressively developed neointima above the underlying residual plaque. Optical coherence tomography suggested developing neovascularization within the neointima. Stentless PCI with drug-coated balloon angioplasty after DCA was re-performed, and collected restenotic sample. The histopathological evaluation confirmed less-cellular neointimal proliferation with rich neovascularization and concomitant diffuse vascular endothelial growth factor (VEGF) expression.

Discussion
Late-phase restenosis post-drug-coated balloon angioplasty after DCA comprised less-cellular neointima, suggesting inhibition of cell proliferation by drug-coated balloon efficacy. However, diffuse VEGF expression and concomitant rich neovascularization with haemorrhage and inflammation might indicate neointimal proliferation. Further large-scale investigations of the restenotic mechanism should be performed to avoid long-term target vascular failure after drug-coated balloon angioplasty post-DCA.
Cardiac tamponade and constrictive pericarditis due to Actinomyces meyeri bacterial pericarditis: a case report
Authors: Eng-Frost Joanne , Murray Lewis , Lorensini Scott , Harjit-Singh Rajinder Singh 
Published: June 2022

Abstract

Background
Purulent bacterial pericarditis (PBP) is a highly lethal infection of the pericardial space that arises as a complication of infective illnesses. Purulent bacterial pericarditis remains a diagnostic challenge given its non-specific clinical and investigative features and carries exceedingly high mortality rates due to fulminant sepsis and morbidity including constrictive pericarditis in survivors. We present our management of cardiac tamponade and subsequent constrictive pericarditis due to Actinomyces meyeri PBP.

Case summary
A 53-year-old Caucasian male presented with acute New York Heart Association Class IV dyspnoea and chest discomfort, in the context of multiple hospital presentations over the preceding 8 weeks due to presumed recurrent viral pericarditis. On this admission, initial transthoracic echocardiography (TTE) demonstrated a large asymmetric pericardial effusion for which he underwent urgent pericardiocentesis. Serial TTE post-pericardiocentesis, however, demonstrated effusion re-accumulation and effusive-constrictive pericarditis, confirmed on cardiac magnetic resonance imaging. Fluid culture was positive for A. meyeri. He was diagnosed with PBP, but his condition deteriorated despite appropriate intravenous antibiotic therapy, necessitating semi-urgent surgical pericardiectomy. He recovered well and was discharged on Day 10 post-operatively.

Discussion
Unlike uncomplicated acute viral or idiopathic pericarditis, PBP portends a very poor prognosis if unrecognized and untreated. Diagnostic challenges persist given its rarity in modern clinical practice; however, PBP should be considered in cases of seemingly recurrent pericarditis. Multi-modal cardiac imaging and careful analysis of pericardial fluid including cultures and lactate dehydrogenase/serum ratios may assist in earlier recognition. In this case, source control and symptom relief were achieved only with combined intravenous antibiotics, surgical evacuation, and pericardiectomy.
Post-operative atrial fibrillation and risk of heart failure hospitalization
Authors: Goyal Parag , Kim Michael , Krishnan Udhay , Mccullough Stephen A , Cheung Jim W , Kim Luke K , Pandey Ambarish , Borlaug Barry A , Horn Evelyn M , Safford Monika M , Kamel Hooman 
Published: June 2022

Abstract

Aims
Post-operative atrial fibrillation (POAF) is associated with stroke and mortality. It is unknown if POAF is associated with subsequent heart failure (HF) hospitalization. This study aims to examine the association between POAF and incident HF hospitalization among patients undergoing cardiac and non-cardiac surgeries.

Methods and results
A retrospective cohort study was conducted using all-payer administrative claims data that included all non-federal emergency department visits and acute care hospitalizations across 11 states in the USA. The study population included adults aged at least 18 years hospitalized for surgery without a prior diagnosis of HF. Cox proportional hazards regression models were used to examine the association between POAF and incident HF hospitalization after making adjustment for socio-demographics and comorbid conditions. Among 76 536 patients who underwent cardiac surgery, 14 365 (18.8%) developed incident POAF. In an adjusted Cox model, POAF was associated with incident HF hospitalization [hazard ratio (HR) 1.33; 95% confidence interval (CI) 1.25–1.41]. In a sensitivity analysis excluding HF within 1 year of surgery, POAF remained associated with incident HF hospitalization (HR 1.15; 95% CI 1.01–1.31). Among 2 929 854 patients who underwent non-cardiac surgery, 23 763 (0.8%) developed incident POAF. In an adjusted Cox model, POAF was again associated with incident HF hospitalization (HR 2.02; 95% CI 1.94–2.10), including in a sensitivity analysis excluding HF within 1 year of surgery (HR 1.49; 95% CI 1.38–1.61).

Conclusions
Post-operative atrial fibrillation is associated with incident HF hospitalization among patients without prior history of HF undergoing both cardiac and non-cardiac surgeries. These findings reinforce the adverse prognostic impact of POAF and suggest that POAF may be a marker for identifying patients with subclinical HF and those at elevated risk for HF.
The RNA-binding protein Swm is critical for Drosophila melanogaster intestinal progenitor cell maintenance
Authors: Ariyapala Ishara S , Buddika Kasun , Hundley Heather A , Calvi Brian R , Sokol Nicholas S 
Published: June 2022

Abstract
The regulation of stem cell survival, self-renewal, and differentiation is critical for the maintenance of tissue homeostasis. Although the involvement of signaling pathways and transcriptional control mechanisms in stem cell regulation have been extensively investigated, the role of post-transcriptional control is still poorly understood. Here, we show that the nuclear activity of the RNA-binding protein Second Mitotic Wave Missing is critical for Drosophila melanogaster intestinal stem cells and their daughter cells, enteroblasts, to maintain their progenitor cell properties and functions. Loss of swm causes intestinal stem cells and enteroblasts to stop dividing and instead detach from the basement membrane, resulting in severe progenitor cell loss. swm loss is further characterized by nuclear accumulation of poly(A)+ RNA in progenitor cells. Second Mitotic Wave Missing associates with transcripts involved in epithelial cell maintenance and adhesion, and the loss of swm, while not generally affecting the levels of these Second Mitotic Wave Missing-bound mRNAs, leads to elevated expression of proteins encoded by some of them, including the fly ortholog of Filamin. Taken together, this study indicates a nuclear role for Second Mitotic Wave Missing in adult stem cell maintenance, raising the possibility that nuclear post-transcriptional regulation of mRNAs encoding cell adhesion proteins ensures proper attachment of progenitor cells.
Use of polymyxins for carbapenem-resistant infections in children and adolescents
Authors: Barco-Cabrera Carolina , Reina Yeison A , Dávalos Diana M , López Pio , Tulcán-Toro Rubén , Cantor Erika , López-Medina Eduardo 
Published: June 2022

Abstract

Background
Polymyxins are still used in children in some regions due to limited availability of newer antibiotics.

Objectives
To describe our experience in a cohort of children who received polymyxins for suspected or confirmed carbapenem-resistant bacterial infections (CRI), and explore potential factors associated with therapeutic success.

Methods
Retrospective, observational study in children and adolescents &lt;18 years who received IV polymyxin B or colistin therapy for suspected or culture-documented CRI and were admitted to a high complexity clinic in Cali, Colombia between 1 September 2016 and 22 June 2020. Patients’ demographic, clinical and microbiological characteristics were collected and analysed; associations with therapeutic success were explored using univariate and multivariate models.

Results
There were 40 episodes of polymyxin use (polymyxin B, n = 34; colistin, n = 6) in 34 patients with a median age of 10 years (IQR 7–15); 65% were male. There were 17 adverse events: 3 (17.6%) neurotoxic and 14 (82.4%) nephrotoxic. Therapeutic success was achieved in 28 episodes (70%), of which 32% (9/28) had adverse events. Therapeutic success decreased by 35% with each additional year of age (OR 0.65; 95% CI 0.49–0.80) and by 7% for every hour that elapsed between the onset of fever and the start of appropriate antibiotic therapy (OR 0.93; 95% CI 0.8–0.97) and increased with concomitant non-carbapenem treatment (OR 6.87; 95% CI 1.04–71.01) and the use of adequate empirical therapy (OR 121.36; 95% CI 2.90–1147.95).

Conclusions
Several factors were associated with the therapeutic success of polymyxins, however, more than half of episodes had therapeutic failure or adverse events. Antibiotics with greater efficacy and safety are needed in regions with high rates of CRI.
A Comparison of Topical Agents for Eschar Removal in a Porcine Model: Bromelain-enriched vs Traditional Collagenase Agents
Authors: Singer Adam J , Goradia Eshani N , Grandfield Samuel , Zhang Nigel , Shah Kunal , McClain Steve A , Sandoval Steven , Shoham Yaron 
Published: June 2022

AbstractSurgical excision and grafting of deep partial-thickness (DPT) and full-thickness (FT) burns is a cornerstone of wound care. The use of commercially available topical enzymatic agents has been limited due to slower and less complete eschar removal than surgical excision. Using a porcine model of DPT and FT burns, we compared the eschar removal efficacy of a bromelain-enriched enzymatic agent derived from the stems of pineapple plants and a commercially available collagenase. We created 40 DPT and 40 FT burns on four anesthetized Yorkshire pigs. Eschar removal was initiated 24 hours later. Two pigs each were randomly assigned to collagenase or the bromelain-enriched agent. The bromelain-enriched agent was applied topically once for 4 hours followed by a 2-hour soaking. The collagenase was applied topically daily until complete removal of eschar or for up to 14 days. All bromelain-enriched treated FT burns underwent complete removal of the eschar after a single application while none of the collagenase-treated FT burns underwent complete removal of the eschar even after 14 days of treatment. All bromelain-enriched treated DPT burns had complete eschar removal after the single application. None of the collagenase-treated DPT burns experienced complete removal of eschar after 10 days; by day 14, 35% had complete eschar removal, 30% had &gt;50% eschar removed, and 35% had &lt;50% eschar removed. We conclude that eschar removal is quicker and more complete with the bromelain-enriched compared with collagenase debriding agent.
Wage variations and commuting distance
Authors: Aboulkacem El-Mehdi , Nedoncelle Clément 
Published: June 2022

Abstract
We estimate the causal impact of wage variations on commuting distance of workers. We test whether higher wages across years lead workers to live further away from their working place. We use employer–employee data for the French Ile-de-France region (surrounding Paris), from 2003 to 2008, and we deal with the endogenous relation between income and commuting using an instrumental variable strategy. We estimate that increases in wages coming from exogenous exposure to trade activities lead workers to increase their commuting distance and to settle closer to the city of Paris historical center. Our results cast novel insights upon the causal mechanisms from wage to spatial allocation of workers.
LIGO–Virgo correlations between mass ratio and effective inspiral spin: testing the active galactic nuclei channel
Authors: McKernan B , Ford K E S , Callister T , Farr W M , O’Shaughnessy R , Smith R , Thrane E , Vajpeyi A 
Published: June 2022

ABSTRACT
Observations by LIGO–Virgo of binary black hole mergers suggest a possible anticorrelation between black hole mass ratio (q = m2/m1) and the effective inspiral spin parameter χeff, the mass-weighted spin projection on to the binary orbital angular momentum. We show that such an anticorrelation can arise for binary black holes assembled in active galactic nuclei (AGNs) due to spherical and planar symmetry-breaking effects. We describe a phenomenological model in which (1) heavier black holes live in the AGN disc and tend to spin-up into alignment with the disc; (2) lighter black holes with random spin orientations live in the nuclear spheroid; (3) the AGN disc is dense enough to rapidly capture a fraction of the spheroid component, but small in radial extent to limit the number of bulk disc mergers; (4) migration within the disc is non-uniform, likely disrupted by feedback from migrators or disc turbulence; (5) dynamical encounters in the disc are common and preferentially disrupt binaries that are retrograde around their centre of mass, particularly at stalling orbits, or traps. Comparisons of predictions in (q, χeff) parameter space for the different channels may allow us to distinguish their fractional contributions to the observed merger rates.
On the formation of massive quiescent galaxies with diverse morphologies in the TNG50 simulation
Authors: Park Minjung , Tacchella Sandro , Nelson Erica J , Hernquist Lars , Weinberger Rainer , Diemer Benedikt , Nelson Dylan , Pillepich Annalisa , Marinacci Federico , Vogelsberger Mark 
Published: June 2022

ABSTRACT
Observations have shown that the star formation activity and the morphology of galaxies are closely related but the underlying physical connection is not well understood. Using the TNG50 simulation, we explore the quenching and the morphological evolution of the 102 massive quiescent galaxies in the mass range of 10.5 &lt; log (Mstellar/M⊙) &lt; 11.5 selected at z = 0. We show that galaxies tend to be quenched more rapidly if they (i) are satellites in massive haloes, (ii) have lower star-forming gas fractions, or (iii) inject a larger amount of black hole kinetic feedback energy. Following global evolutionary pathways, we conclude that quiescent discs are mainly disc galaxies that are recently and slowly quenched. Approximately half of the quiescent ellipticals at z = 0 are rapidly quenched at higher redshifts while still disc-like. While quiescent, these gradually become more elliptical mostly by disc heating, yet these ellipticals still retain some degree of rotation. The other half of quiescent ellipticals with the most random motion-dominated kinematics build up large spheroidal components before quenching primarily by mergers, or in some cases, misaligned gas accretion. However, the mergers that contribute to morphological transformation do not immediately quench galaxies in many cases. In summary, we find that quenching and morphological transformation are largely decoupled. We conclude that the TNG black hole feedback – in combination with the stochastic merger history of galaxies – leads to a large diversity of quenching time-scales and a rich morphological landscape.
Gap opening by planets in discs with magnetized winds
Authors: Elbakyan Vardan , Wu Yinhao , Nayakshin Sergei , Rosotti Giovanni 
Published: June 2022

ABSTRACT
Planets open deep gaps in protoplanetary discs when their mass exceeds a gap opening mass, Mgap. We use one- and two-dimensional simulations to study planet gap opening in discs with angular momentum transport powered by MHD disc winds. We parametrize the efficiency of the MHD disc wind angular momentum transport through a dimensionless parameter αdw, which is an analogue to the turbulent viscosity αv. We find that magnetized winds are much less efficient in counteracting planet tidal torques than turbulence is. For discs with astrophysically realistic values of αdw, Mgap is always determined by the residual disc turbulence, and is a factor of a few to ten smaller than usually obtained for viscous discs. We introduce a gap opening criterion applicable for any values of αv and αdw that may be useful for planet formation population synthesis. We show that in discs powered by magnetized winds growing planets detach from the disc at planet masses below $\sim 0.1{\, {\rm M}_{\rm J}}$ inside 10 au. This promotes formation of super-Earth planets rather than gas giants in this region, in particular precluding formation of hot jupiters in situ. On larger scales, ALMA gap opening planet candidates may be less massive than currently believed. Future high-resolution observations with instruments such as the extended ALMA, ngVLA, and SKA are likely to show abundant narrow annular features at R &lt; 10 au due to ubiquitous super-Earth planets.