bluuebunny/crossref_metadata_2025_split huggingface dataset:
Facilitating high throughput collections-based genomics: a comparison of DNA extraction and library building methods
Authors: Marsh William A , Hall Andie , Barnes Ian , Price Ben 
Published: February 2025

Abstract
While DNA barcoding methods are an increasingly important tool in biological conservation, the resource requirements of constructing reference libraries frequently reduce their efficacy. One efficient way of sourcing taxonomically validated DNA for reference libraries is to use museum collections. However, DNA degradation intrinsic to historical museum specimens can, if not addressed in the wet lab, lead to low quality data generation and severely limit scientific output. Several DNA extraction and library build methods that are designed to work with degraded DNA have been developed, although the ability to implement these methods at scale and at low cost has yet to be formally addressed. Here, the performance of widely used DNA extraction and library build methods are compared using museum specimens. We find that while our selected DNA extraction methods do not significantly differ in DNA yield, the Santa Cruz Reaction (SCR) library build method is not only the most effective at retrieving degraded DNA from museum specimens but also easily implemented at high throughput for low cost. Results highlight the importance of lab protocol on data yield. An optimised “sample to sequencing” high-throughput protocol which incorporates SCR is included to allow for easy uptake by the wider scientific community.
Synergistic and antagonistic activities of IRF8 and FOS enhancer pairs during an immune-cell fate switch
Authors: Klonizakis Antonios , Alcoverro-Bertran Marc , Massó Pere , Thomas Joanna , de Andrés-Aguayo Luisa , Wei Xiao , Varamogianni-Mamatsi Vassiliki , Nikolaou Christoforos , Graf Thomas 
Published: February 2025

Abstract
Cell fate instructive genes tend to be regulated by large clusters of enhancers. Whether and how individual enhancers within such clusters cooperate in regulating gene expression is poorly understood. We have previously developed a computational method, SEGCOND, which identifies hubs that we termed Putative Transcriptional Condensates (PTCs), consisting of enhancer clusters and associated target genes. Here, we use SEGCOND to identify PTCs in a CEBPA-induced B-cell-to-macrophage transdifferentiation system. We find that PTCs are enriched for highly expressed, lineage-restricted genes and associate with BRD4, a component of transcriptional condensates. Further, we performed single and combinatorial deletions of enhancers within two PTCs active during induced transdifferentiation, harboring IRF8 and FOS. Two enhancers within the IRF8 PTC were found to provide a backup mechanism when combined, safeguarding IRF8 expression and efficient transdifferentiation. Unexpectedly, two individual enhancers within the FOS PTC antagonize each other on day 1 of transdifferentiation, delaying the conversion of B-cells into macrophages and reducing FOS expression, while on day 7, they cooperate to increase FOS levels induced cells. Our results reveal complex, differentiation-stage-specific interactions between individual enhancers within enhancer clusters.
Cu2+ substitution regulating Na3V2(PO4)3 with solid SEI membrane for superior electrochemical performance
Authors: Peng Zhenbo , Chen Bifen , Yu Shan , Wu Kaiyu , Zhang Farao , Gao Peng 
Published: February 2025

Cu2+ substitution introduces p-type doping effects to supply hole carriers and plays pillar effect to support crystal skeleton.
Comprehensive Insights into Electrochemical Nicotine Sensing Technologies
Authors: P K Udhaya Ganesh , Sagayaraj Prince J.J. , Maruthapillai Arthanareeswari , Kim Hyoung-il , Sekar Karthikeyan , Gunasekaran Sundaram 
Published: February 2025

Nicotine is a significant alkaloid prevalent in tobacco products. Given the addictive nature of tobacco products and the health risks associated with their consumption, precise and real-time monitoring of nicotine is...
“I'm Never Gonna Go Back So I've Gotta Do It Forward”: Exploring Posttraumatic Growth in Aphasia
Authors: Harmon Tyson G. , Williams Camille , Brancamp Tami , Hambridge Trish , Wallace Sarah E. , Evans William , Biel Michael , Cavanaugh Robert , Caputo Mike 
Published: February 2025

Purpose:
The aim of this study was to explore posttraumatic growth (PTG) in people with aphasia.

Method:
As part of a larger multisite study, 23 people with aphasia (12 females, 11 males) each completed a 60-min semistructured interview during which they expanded on previously given questionnaire responses and then answered five additional open-ended questions about communication in their daily life. Interviews were transcribed orthographically, coded using reflexive codebook analysis, and synthesized into themes and categories by team members that included a person with aphasia.

Results:
Reflexive codebook analysis revealed two themes. The first theme, “Moving Toward Growth” described the process of PTG, which included categories of “Grappling With New Reality,” “Acceptance,” “Goals and Effort,” and “Improvement.” The second theme identified “Perceived Areas of Growth,” which included categories of “Perception of Self,” “Relatedness,” and “General Philosophy of Life.”

Conclusions:
Findings suggest that people with aphasia experience PTG across all three primary domains: (a) changed perception of self, (b) changed relationship with others, and (c) changed general philosophy of life and that the challenging circumstances associated with aphasia were integral to the development of PTG. Findings also highlight that PTG does not necessarily develop in a linear trajectory for people with aphasia and that processes such as acceptance, exerting effort, striving for continued improvement, and slowing down may contribute to the overall development of PTG in this population. Overall, the construct of PTG is relevant to people with aphasia, and the process by which PTG is developed in people with aphasia involves similar components to what has been described in other populations.

Supplemental Material:

https://doi.org/10.23641/asha.28394993
Research and Improvement of Muller C Element
Authors: Chen Zhizhi , Wang Qian , Chen Houpeng , Li Xi , Chen Xiaogang , Song Sannian , Song Zhitang 
Published: February 2025

ABSTRACTThe basic structure of conventional Muller C element is studied and the output errors caused by node parasitic capacitors are analysed in this letter. In order to avoid such errors, an improved C element with two inverted ratio MOS transistors added is proposed to control high resistance nodes of the circuit. To reduce the power consumption of C element, two resistors are added. The proposed C element has been designed in 28 nm CMOS process and 2.5 V devices are adopted. The simulation results show that proposed C element avoids such errors and the size of the layout is 67.33 µm2.
Enhancing the localization of gravitational-wave hosts with SKYFAST: Rapid volume and inclination angle reconstruction
Authors: Demasi Gabriele , Capurri Giulia , Ricciardone Angelo , Patricelli Barbara , Lenti Massimo , Del Pozzo Walter 
Published: February 2025

Context. The scientific impact of GW170817 strongly supports the suggestion that we need an efficient electromagnetic follow-up campaign for gravitational-wave event candidates. The success of these campaigns critically depends on a fast and accurate localization of the source.
Aims. We present SKYFAST, an algorithm for the rapid identification of gravitational-wave hosts to optimize electromagnetic follow-up searches. The goal is to produce a list of the galaxies within the localization volume, ranked by their probability of being the host, along with an estimate of the inclination angle conditioned on the position of each galaxy.
Methods.SKYFAST runs alongside a full parameter estimation (PE) algorithm, from which posterior samples are taken. These samples are then used to reconstruct an analytical posterior of the sky position, luminosity distance, and inclination angle using a Dirichlet process Gaussian mixture model, which is a nonparametric Bayesian method.
Results. We show that SKYFAST can reconstruct an accurate localization using only a fraction (∼10%) of the total posterior samples produced by the PE. Moreover, SKYFAST generates a ranked list of the most probable hosts from a galaxy catalog of choice in a few minutes. This list includes information on the inclination angle posterior conditioned on the position of each candidate host. This breaks the degeneracy between inclination angle and luminosity distance.
Conclusions. The reconstruction of the posterior using fewer samples than the full PE can lead to significant time savings, depending on the PE algorithm employed. This is crucial for identifying the electromagnetic counterpart. The inclusion of the inclination angle information conditioned on the position of each galaxy can lead to an optimized electromagnetic follow-up.
Rehabilitation beim Long-/Post-COVID-Syndrom: ein
bewegungstherapeutisches Handlungskonzept
Authors: Köppel Maximilian , Schwan Uwe , Peters Stefan , Späth Tilo , Huber Gerhard 
Published: February 2025

ZusammenfassungDank flächendeckender Impfungen konnten die COVID-19-bedingte Letalität und
Hospitalisierungsrate substanziell reduziert werden. Dennoch sind die
Langzeitfolgen der COVID-19-Infektionen damit nicht beseitigt. Etwa die Hälfte
der Betroffenen berichtet auch nach Ende der Infektion von persistierenden
Symptomen, dem Long-COVID- bzw. Post-COVID-Syndrom (LCS/PCS). Zwar können einige
Kardinalsymptome des LCS/PCS ausgemacht werden, aber keineswegs alle Betroffenen
leiden unter diesen. Auch ansonsten bleibt das Krankheitsbild sehr heterogen und
kann in Extremfällen in einer schweren, der Myalgischen Enzephalomyelitis
(Chronisches Fatigue-Syndrom) vergleichbaren Symptomatik münden. Dank ihrer
vielseitigen Wirkungen und Wirkmechanismen stellt die Bewegungstherapie eine
sinnvolle Maßnahme dar, dieses heterogene Krankheitsbild zu adressieren. Hier
ist jedoch kein Vorgehen nach „Schema F“, sondern ein differenzierter, hoch
individualisierter Ansatz zu verfolgen.
Thinking out of the tube: Endoskopische Befunde in Mundhöhle, Pharynx und Larynx im Rahmen der flexiblen Ösophagogastroduodenoskopie
Authors: Gundling Felix , Schad Christopher Moritz , Böss Nadia , Wannhoff Andreas , Becker Eva-Tessina , Eckert Andreas , Pech Oliver 
Published: February 2025

ZusammenfassungWährend einer flexiblen Ösophagogastroduodenoskopie (ÖGD) können in der Regel die Mundhöhle, Oropharynx, Hypopharynx und Larynx mit einer hohen optischen Auflösung eingesehen werden. In diesem Abschnitt des Verdauungstrakts kommen zahlreiche benigne und maligne Befunde von klinischer Relevanz vor, welche im Rahmen einer Gastroskopie diagnostiziert und einer entsprechen Therapie zugeführt werden können. Insbesondere gilt dies für Kopf-Hals-Tumore, welche eine heterogene Gruppe bösartiger Erkrankungen darstellen und bis ca. 4% der in Deutschland diagnostizierten Krebserkrankungen des Mannes ausmachen.In dieser Übersichtsarbeit werden benigne und maligne Befunde in Mundhöhle, Pharynx- und Larynxbereich exemplarisch gezeigt, welche während der gastroenterologischen Routine-Endoskopie erhoben wurden, und in den Kontext einer ausführlichen Literaturrecherche gestellt.Benigne und maligne Befunde in Mundhöhle, Oropharynx, Hypopharynx und Larynx sind mit einem vertretbaren zusätzlichen Zeitaufwand optisch einfach zu diagnostizieren. In ausgewählten Fällen ist eine histologische Sicherung möglich. Wir beschreiben einen Vorschlag für einen strukturierten Untersuchungsablauf.Durch eine sorgfältige routinemäßige Inspektion von Mundhöhle, Oropharynx, Hypopharynx und Larynx im Rahmen der Gastroskopie können vor allem bei Risikopersonen, aber auch in der Allgemeinbevölkerung, pathologische Befunde dargestellt und potenziell im Frühstadium diagnostiziert werden.
Green Tea Catechins: A Promising Anticancer Approach for Leukaemia
Authors: Mazur Łukasz , Balwierz Radosław , Michalak Krzysztof , Michalak Wojciech , Jasińska-Balwierz Agata , Shanaida Mariia , Biernat Paweł , Baj Tomasz , Jasicka-Misiak Izabela 
Published: February 2025

AbstractGreen tea catechins are bioactive polyphenolic compounds that possess a number of biological activities and potential health benefits. This review will focus on discussing the effects of green tea catechins, with a particular emphasis on clinical studies that evaluate their anticancer potential. Epigallocatechin gallate (EGCG), either as a stand-alone treatment or in conjunction with conventional anticancer therapies, represents a promising alternative strategy for the management of leukaemia. This review was based on a search of the scientific sources indexed in the databases PubMed and Scopus using the following keywords: ‘Camellia sinensis’, ‘tea catechins’, ‘anticancer’, ‘antioxidant’, ‘hematological cancer’, and ‘leukaemia’ in combination. A deeper comprehension of the multifaceted mechanisms and findings of research could facilitate the development of novel strategies and the integration of green tea catechins into clinical practice, thus enhancing treatment
outcomes for patients with leukaemia.
Photoinduced Dearomative Multiple Functionalization of Quinolines to Construct Highly Substituted 3D Frameworks
Authors: Nagashima Yuki , Shimose Asuha 
Published: February 2025

AbstractDearomative functionalization of quinolines expands the chemical diversity of highly functionalized 3D frameworks, such as 1,2,3,4- and 5,6,7,8-tetrahydroquinoline derivatives, which are important pharmacophores, with minimal synthetic costs. In this short review, we cover recent reports on the visible-light-induced dearomative functionalization of quinolines with an emphasis on reaction design/strategies and mechanistic studies and provide a theoretical basis for developing further dearomative syntheses in the future.1	Introduction2	Dearomative Functionalizations through the Formation of Photoexcited Quinolines3	Dearomative Functionalizations through the Formation of Photoexcited Reagents/Intermediates4	Conclusion
Vector magnetometry using shallow implanted NV centers in diamond with waveguide-assisted dipole excitation and readout
Authors: Shahbazi Sajedeh , Coccia Giulio , Giakoumaki Argyro N. , Lang Johannes , Bharadwaj Vibhav , Jelezko Fedor , Ramponi Roberta , Bennett Anthony J. , Hadden John P. , Eaton Shane M. , Kubanek Alexander 
Published: February 2025

On-chip magnetic field sensing with nitrogen-vacancy (NV) centers in diamond requires scalable integration of 3D waveguides into diamond substrates. Here, we develop a sensing array device with an ensemble of shallow implanted NV centers integrated with arrays of laser-written waveguides for excitation and readout of NV signals. Our approach enables an easy-to-operate on-chip magnetometer with a pixel size proportional to the Gaussian mode area of each waveguide. The performed continuous wave optically detected magnetic resonance on each waveguide gives an average dc-sensitivity value of (195±3) nT/Hz, which can be improved with lock-in-detection or pulsed-microwave sequences. We apply a magnetic field to separate the four NV crystallographic orientations of the magnetic resonance and then utilize a DC current through a straight wire antenna close to the waveguide to prove the sensor capabilities of our device. We reconstruct the complete vector magnetic field in the NV crystal frame using three different NV crystallographic orientations. By knowing the polarization axis of the waveguide mode, we project the magnetic field vector into the lab frame.
Characterizing the photodissociation dynamics of HPCO in the S1 band
Authors: Li Jiayuan , Hou Siting , Xie Changjian 
Published: February 2025

A full-dimensional potential energy surface (PES) represented by the neural network method for the first excited state S1(1A″) of HPCO is reported for the first time. The PES was constructed based on more than 51 000 ab initio points, which were calculated at the multi-reference configuration interaction level with Davidson correction using the augmented correlation consistent polarized valence triple zeta basis set. Based on the newly constructed PES, quasi-classical trajectory calculations were carried out to study the photodissociation dynamics of HPCO at the total energy ranging from 4.0 to 5.6 eV. At low total energies, the HP + CO product is dominant, while the product H + PCO becomes increasingly favored at higher energies. Furthermore, the translational energy distributions of two products are found to be energy-dependent. Owing to the strongly repulsive PES along the HP + CO dissociation pathway, the translational energy distributions of HP + CO are dominated by relatively higher energies in contrast to H + PCO. The diatomic products HP and CO are found to possess the vibrational distributions decaying monotonically with the vibrational quantum number and relatively cold rotational state distributions, consistent with the strongly repulsive potentials toward the HP + CO channel. In addition, the vibrational distributions of HP and CO are found to be quite similar due to their close frequencies, while the rotational distributions of CO have a much more highly excited rotational degree of freedom owing to its rotational constant approximately four times smaller than that of HP.
Effect of fire severity and presence of bamboo (Chusquea culeou) on soil chemical properties in Andean Patagonian forests of Argentina
Authors: Urretavizcaya M. Florencia , Rago M. Melisa , Caselli Marina , Ríos Campano Fernanda , Gianolini Stefano , Alonso Virginia 
Published: February 2025

Background Fire is recurrent in Patagonian forests of Argentina, and is frequently associated with the die-off of bamboo (Chusquea culeou). Fire induces changes in soil properties, which may affect vegetation recovery. Aims This study assesses how fire severity affects soil chemical properties in different Patagonian forests, in the absence and presence of C. culeou. Methods We collected samples from the upper 5 cm of mineral soil from 44 plots distributed in five forest types affected by low-severity and high-severity fire, and unburned areas. Half of the plots also had C. culeou. Key results Fire severity affected soil pH, organic matter, electrical conductivity, nitrogen, C:N ratio, and cation exchange capacity. Changes in organic matter and C:N ratio varied among forest type, and variations in pH and sodium were detected among forest types. The presence of C. culeou affected soil pH, C:N ratio, and calcium. Conclusions The changes recorded were expected and could have a lasting effect on long-term productivity. Nothofagus pumilio and Nothofagus antarctica, and forests with C. culeou, varied in their soil chemical properties, sometimes regardless of fire. Implications Considering the heterogeneity of vegetation and soil in postfire environments is vital for predicting possible successional trajectories and defining restoration objectives.
Joint measurement of temperature, pressure and water height as a function of time in the candle covered by a glass experiment
Authors: Garcia-Molina Rafael , Tomás-Serrano Antonio 
Published: February 2025

Abstract
Rising of liquid inside a glass covering a candle situated on a plate containing water, when the flame is extinguished, is a classical experiment whose justification relies more on physical than on chemical arguments, despite a widely extended wrong explanation based on the reduction of gas moles due to the combustion. The well-known correct explanation of rising water, based on the initial air expansion inside the glass, being heated by the candle flame, and subsequent pressure reduction after the flame is extinguished, is carefully analysed in this work by jointly measuring the variation with time of water height and entry velocity, temperature and pressure inside the glass. These measurements show that the evolution of the water height and entry velocity correlates with that of the pressure inside the glass through a four stages model.
Multistability and multi-spiral chaotic sea in a novel 3-D system with a line of equilibrium
Authors: Zaamoune Faiza , Tinedert Imad Eddine , Menacer Tidjani , Wang Ning 
Published: February 2025

Abstract
This paper introduces a novel chaotic system that features a line of equilibrium and a unique multi-spiral chaotic sea. Unlike classical conservative chaotic systems, this model cannot directly exhibit a zero divergence but its conservative property can be confirmed by the zero sum of Lyapunov exponents. In addition, the system is shown to support the generation of coexisting nested hidden tori under specific parameter and initial conditions. The detailed dynamic analysis is presented using bifurcation diagrams, Lyapunov exponent, and phase portrait. The corresponding analog circuit is designed with circuitry simulations results to verify the theoretical predictions and numerical simulations.
Quasiperiodic arrangement of magnetodielectric δ-plates: Green’s functions and Casimir energies for N bodies
Authors: Abhignan Venkat 
Published: February 2025

Abstract
We study a variety of ﬁnite quasiperiodic conﬁgurations with magnetodielectric δ-function plates created from simple substitution rules. While previous studies for N bodies involved interactions mediated by a scalar ﬁeld, we extended our analysis of Green’s function and corresponding Casimir energy to the electromagnetic ﬁeld using plates with magnetic and dielectric properties for handling ﬁnite-size quasiperiodic lattices. The Casimir energy is computed for a class of quasiperiodic structures built from N purely conducting or permeable δ-plates. The Casimir energy of this quasiperiodic sequence of plates turns out to be either positive or negative, indicating that the pressure from the quantum vacuum tends to cause the stack of plates to expand or contract depending on their arrangement. We also handle the transverse electric and transverse magnetic mode Green’s functions for δ-plates and derive the Faddeev-like equation with the transition matrix for N purely conducting or permeable plates.&#xD;
Equivalence in virtual transitions between uniformly accelerated and static atoms: from a bird's eye
Authors: Kumawat Pradeep Kumar , Barman Subhajit , Majhi Bibhas Ranjan 
Published: February 2025

Abstract
We study the prospect of the equivalence principle at the quantum regime by investigating
the transition probabilities of a two-level atomic detector in different scenarios. In particular,
two specific set-ups are considered. (i) Without a boundary: In one scenario the atom is
in uniform acceleration and interacting with Minkowski field modes. While in the other the atom is
static and in interaction with Rindler field modes. (ii) With a reflecting boundary: In
one scenario the atom is uniformly accelerated and the mirror is static, and in the other scenario
the atom is static and the mirror is in uniform acceleration. In these cases, the atom interacts
with the field modes, defined in the mirror's frame. For both the set-ups, the focus is on the
excitation and de-excitation probabilities in (1+1) and (3+1) spacetime dimensions. Our
observations affirm that in (1+1) dimensions, for both set-ups the transition probabilities from
different scenarios become the same when the atomic and the field frequencies are equal. In
contrast, in (3+1) dimensions this equivalence is not observed in general, inspiring us to look
for a deeper physical interpretation. Our findings suggest that when the equivalence between
different scenarios is concerned, the excitation to de-excitation ratio provides a more consistent
measure even in (3+1) dimensions. We discuss the physical interpretation and implications of our
findings.