bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bilateral asymmetrical hip dislocation: a case report and literature review
Authors: Mirhoseini Mohammadsajad , Gargari Omid Kohandel 
Published: June 2022

Abstract
Background
Asymmetrical bilateral dislocation of hip is dislocation of both hips in different directions, one hip dislocates anteriorly and the other one dislocates posteriorly. 90 percent of all hip dislocations are unilateral and posterior. Asymmetrical bilateral dislocations are very rare. there is no study comparing asymmetrical bilateral dislocation and unilateral dislocations in detail. AVN (Avascular necrosis) is the most important complication of hip dislocation and the time interval between injury and reduction is crucial to avoid avascular necrosis.
Case presentation:
We are reporting a case of asymmetrical bilateral dislocation of the hip in a 60 years old man after falling from 6 meters height. 4 hours after injury, the patient was transferred to the operation room, and the dislocation was close reduced
Literature review:
A literature review was done to compare sexual distribution, mechanism of dislocation, and associated fractures between patients with asymmetrical bilateral dislocation of hip (n = 103) and unilateral dislocation of hip (n = 105). The results revealed that the female to male ratio is almost the same in both groups, although most patients were male in our population (81%). Traffic accidents are the most common cause of dislocation. Nevertheless, the odds of this mechanism are about 2 times larger in patients with asymmetrical bilateral dislocation (OR = 0.46). Fractures were more common among patients with asymmetrical bilateral dislocation, and the most common associated fractures included: Acetabulum (52.8%), posterior edge (21.3%), femur neck (18.5%), femur head (3.7%), and pelvis (3.7%).
Conclusion
Associated fractures are more common among patients with asymmetrical bilateral hip dislocation so have higher risk for long-term complications.
Spatiotemporal depletion of groundwater level in a drought-prone Rangpur district, northern region of Bangladesh
Authors: Monir Md. Moniruzzaman , Sarker Subaran Chandra , Sarkar Showmitra Kumar , Ahmed Mohd. , Mallick Javed , I Abu Reza Md. Towfiqul 
Published: June 2022

Abstract
Water insufficiency during the pre-monsoon season and rapidly falling underground freshwater levels is a common scenario in the towns and cities of Bangladesh. The objective of the study is to investigate the spatiotemporal depletion of groundwater levels (GWL) in a drought-prone Rangpur district, northern region of Bangladesh. Groundwater observation well data from 1980 to 2019 was used for this purpose. Rainfall, temperature, irrigation, and land use/land cover datasets are used to assess the factors influencing groundwater level depletion. Linear regression, and the least square regression model were used to identify the trend of GWL depletion. Results show that GWL in drought-prone regions is declining rapidly because of the excessive withdrawal of groundwater and land-based reservoirs for irrigation and household needs. The declining GWL is alarming during the pre-monsoon and post-monsoon seasons due to some disproportional mismanagement in the study area. Among the rates of decline, the trend in maximum depth has a comparatively higher rate than the others. Findings from the study show that severe withdrawal of groundwater in agriculture, a decreasing amount of annual rainfall, and degradation of climatic factors in the area are all attributed to a lowering trend in groundwater level.
GRWD1 overexpression promotes the proliferation, migration, and invasion of gastric cancer through the Notch signaling pathway
Authors: Ding Huiming , Feng Zhenyou , Hu Kongwang 
Published: June 2022

Abstract
Background
GRWD1 is a multifunctional protein that is overexpressed in various cancer cell lines and is considered as a potential oncogene. However, its role and mechanism in the occurrence and development of gastric cancer (GC) remains unknown. In this study, we analyzed the expression, biological function and the downstream signaling of GRWD1 in GC.
Methods
Methods: The protein expression of GRWD1 was examined by online data analysis website,IHC and WB. The relationship between GRWD1 expression and various clinical factors in gastric cancer patients was analyzed. To verify whether the expression of GRWD1 affects the postoperative survival time of gastric cancer patients. The effects of GRWD1 on gastric cancer cell proliferation, migration and invasion were verified by CCK8, clone formation assay, wound healing assay, and transwell assay. The relationship between GRWD1 and NOTCH signaling pathway was verified by WB experiments. The effect of GRWD1 on the proliferation of gastric cancer in vivo was verified by tumorigenic experiments in nude mice.
Results
The expression of GRWD1 in GC tissues was higher than that in normal tissues, and was positively correlated with tumor size, lymph node metastasis and TNM stage, and negatively correlated with differentiation degree and prognosis. The overexpression of GRWD1 can promote gastric cancer cell proliferation, migration and invasion by activating NOTCH signaling pathway. The low expression of GRWD1 can inhibit the proliferation, migration and invasion of gastric cancer cells through NOTCH signaling pathway
Conclusion
Our study proves that GRWD1 plays a key role in the occurrence and development of GC. These findings suggest that GRWD1 may be a novel prognostic biomarker and therapeutic target.
The changes of cytokine levels are involved chemotherapy-induced cognitive impairment in non- Hodgkin’s lymphoma Patients
Authors: Li Haowu , Cheng Huaidong , Cui Yue , Lin Guohe , Gao Mingzhu , Xuan Han 
Published: June 2022

Abstract
Purpose
The purpose of this study was investigating the correlation between cytokine levels and chemotherapy-induced cognitive impairment in non-Hodgkin’s lymphoma (NHL) patients.
Methods
A total of 30 patients with NHL were included in this study. These patients completed cognitive assessment and cytokine levels which including IL-4 and IL-6 before chemotherapy and after 2 courses of chemotherapy.
Results
The subjects' IL-4 was higher before treatment than after treatment (t = 2.85, P < 0.01) and IL-6 was lower before treatment than after treatment (Z= -3.17, P < 0.01). IL-6 has a weak correlation between RM and PM (r = 0.282 and 0.270, respectively, all P < 0.05). There was a moderate correlation between IL-6 and ECog-12 (r = 0.314, P < 0.05), but no statistical association was found between cognition and IL-4. There was a moderate correlation between subjects' cognition (MMSE, VFT, SDMT, CDT, RM) and KPS (r = 0.479,0.378, 0.464, 0.396 and − 0.366, respectively, all P < 0.01).
Conclusion
The cognitive function of NHL patients is impaired in a short time after chemotherapy, which is manifested in visuospatial disturbance, language and memory. IL-4 may be a protective factor of cognitive function, and IL-6 may be a factor of impairment of cognitive function. Management and improvement of cognitive function in NHL will help to improve KPS levels.
Trial registration number(date)
YX2021-011(2021/2/18)
Cultivating Interlanguage Pragmatic Comprehension through Concurrent and Cumulative Group Dynamic Assessment: A Mixed-methods Study
Authors: Rezai Afsheen 
Published: June 2022

Abstract
This study explored the effects of concurrent G-DA and cumulative G-DA on Iranian pre-intermediate EFL learners’ ILP comprehension. To this end, a total of 97 pre-intermediate learners took the key English test (KET) test and 45 learners whose scores fell between − 1 and + 1 SD were selected and assigned randomly into three groups, namely concurrent G-DA (n = 15), cumulative G-DA (n = 15), and immediate feedback (IF) group (n = 15). Then, they went through pre-test, intervention (lasted one hour for fifteen sessions held three times a week), and post-test procedures, as well as the interactions among the teachers and learners were recorded. The data were analyzed through a one-way ANCOVA and a microgenetic development approach. Results revealed that the experimental groups outperformed the control group concerning the gains of the ILP comprehension. Additionally, the complementary microgenetic findings demonstrated how concurrent G-DA and cumulative G-DA approaches could lead to improving the learners’ ILP comprehension.
Comparative Bioavailability Assessment of Four Brands of Ciprofloxacin Tablets Marketed in South-East Nigeria
Authors: Nduka Sunday , Opayemi Opeyemi , Mbachu Nancy 
Published: June 2022

Abstract
Objective of study: This study evaluated the relative bioavailability of four brands of ciprofloxacin (500mg) tablets marketed in Southeast, Nigeria using an in vivo approach. Methods: The bioavailability study was carried out on twelve healthy male rabbits which were given a single dose of ciprofloxacin (500mg) tablet of the reference (R) and three test (A, B, D) products in the fasting state in a balanced incomplete block design. Serial blood samples were collected after dosing at 8 different time intervals for a period of 24 hours. Serum obtained from blood was analysed for ciprofloxacin with microbiology assay while plasma samples were analysed using UV-Vis spectrophotometric analytical method at a wavelength of 272.4 nm. The pharmacokinetic parameters were determined using non-compactmental as implemented in WinNonlin pharmacokinetic program. The 90% confident interval of the log transformed mean values of the test/reference ratios for the pharmacokinetic parameters (Cmax), (AUC0-t), ant the (AUC0-∞)were calculated and used to determine the bioequivalence acceptance range of 80 – 125%. Result: The pharmacokinetic parameters from the UV-Vis analytical method showed no statistically significant difference between the reference and the tested brands as the mean Cmax obtained for the reference sample (R) was the highest (275.50 (±3.86) µg/ml) occurring at a tmax of 6.83 (±1.89) h, followed by B (222.17 (±2.11) µg/ml) at 5.08 (±0.98) h, C (202.00 (±0.55) µg/ml) at 3.63 (±1.02) h and A (192.00 (±1.10) µg/ml) at 7.00 (±1.11) h. The value obtained for the 90% CI for log-transformed ration for Cmax is A/R (94.7, 101.6), B/R (87.8, 101.6), C/R (91.2, 99.2) which is within the acceptable range of 80% to 125% as it is for AUC0-t and AUC0-∞also. The obtained outcome for microbiological assay reflected similarities with the UV-Vis assay as the pharmacokinetic parameters are not different statistically and the value obtained for the 90% CI for log-transformed ration for Cmax,AUC0-t and AUC0-∞are within the regulatory acceptable range.Conclusion: The four formulations were considered to be bioequivalent as the peak plasma concentration and AUC parameter analysis of variance reflected no significant difference between the innovator brand and the three other brands.
Sulfatase2 affects the polarization of M2 macrophages through the IL-8/JAK2/STAT3 pathway in bladder cancer
Authors: Zhang Wentao , Yang Fuhan , Zheng Zongtai , Li Cheng , Mao Shiyu , Wu Yuan , Wang Ruiliang , Zhang Junfeng , Zhang Yue , Wang Hong , Li Wei , Huang Jianhua , Yao Xudong 
Published: June 2022

Abstract
Background: Sulfatase 2 (SULF2) can affect the occurrence and development of cancer by regulating HSPG-binding factors. In bladder cancer(BCa), the mechanism of SULF2 is unknown. Here, we clarify the mechanism that SULF2 affects the secretion of HSPG-binding factors (IL-8) and regulates the polarization of M2 macrophages in the BCa microenvironment.Methods: The RNA sequencing of 90 patients with BCa was analyzed. GSEA analyze the relationship between SULF2 and clinicopathological characteristics and prognosis. SULF2 knockdown or overexpression cell lines, flow cytometry, CCK8, Transwell, Edu assay, Western Blot, qPCR, co-culture systems were used to analyze the effects of SULF2 on the biological functions of BCa in vitro. The mouse orthotopic BCa model, flow cytometry and IHC were used to analyze the changes of SULF2 on the tumor microenvironment of BCa in vivo.Results: RNA sequencing data analysis showed that the expression of SULF2 is closely related to the prognosis. Knockdown or overexpression of SULF2 revealed that it promotes tumor proliferation and invasion through in vivo and in vitro experiments. Furthermore, SULF2 affected the polarization of macrophages by using the mouse orthotopic BCa and flow cytometric analysis,. Mechanism studies clarify that SULF2 promotes the release of HSPG-binding factors--IL-8 to the microenvironment through β-catenin. Meanwhile, IL-8 activated the JAK2/STAT3 pathway of macrophages to promote the expression of CD163 and CD206, thereby regulating the polarization of macrophages to M2 macrophages.Conclusions: These data indicate that SULF2 plays an important role in regulating the microenvironment of BCa. SULF2 promotes the polarization of macrophages to M2 by secreting IL-8, which further deepens the malignant progression of BCa.
Microsatellites for the at-risk Mottled Duskywing butterfly, Erynnis martialis
Authors: Kroeze Shayla L. , Brewster Adrienne , Linton Jessica E. , Norris D. Ryan , Otis Gard W. , Young Yvonne , Keyghobadi Nusha 
Published: June 2022

Abstract
We describe 24 microsatellite loci for an at-risk North American butterfly, the Mottled Duskywing (Erynnis martialis). Forty-one perfect tetra and di-nucleotide loci were originally identified using Illumina sequencing and were refined to 24 variable and cleanly amplifiable loci, which we attempted to characterized in 34 individuals from a single population. We detected 2 to 10 alleles per locus. Observed heterozygosity ranged from 0.06 to 0.76, and expected heterozygosity ranged from 0.06 to 0.79. We also describe conditions by which 18 microsatellites can be multiplexed in five separate reactions. These microsatellites will be used to investigate population differentiation and diversity of Mottled Duskywing, to inform reintroduction and conservation protocols for the species in Ontario, Canada where it is listed as endangered.
Experiences participating in federal nutrition assistance programs during the early months of the COVID-19 pandemic: A mixed methods study in Vermont
Authors: Spence Emma H. , Niles Meredith T. , Bertmann Farryl , Belarmino Emily H. 
Published: June 2022

Abstract

Background
Federal nutrition assistance programs serve as safety nets for many American households, and participation has been linked to increased food security and, in some instances, improved diet quality and mental health outcomes. The COVID-19 pandemic brought new and increased economic, social, and psychological challenges, necessitating inquiry into how nutrition assistance programs are functioning and associated public health outcomes.
Methods
Using data from a representative statewide survey administered in Vermont (n = 600) during the early months of the COVID-19 pandemic, we examine participant experiences with the 3 major federal nutrition assistance programs: the Supplemental Nutrition Assistance Program (SNAP), the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC), and school meal programs. We explore quantitative and qualitative responses regarding perceptions of program utility, and use nearest neighbors matching analyses in combination with bivariate statistical tests to assess associations between program participation and food insecurity, perceived stress, and fruit and vegetable intake as indicators of dietary quality.
Results
One in four respondents (27.3%) used at least one federal nutrition assistance program. As compared to non-participants, we find higher rates of food insecurity among program participants (57.5% vs. 18.1%; p < .001), an association that persists even when we compare similar households using matching techniques (p ≤ .001). From matched analyses, we find that, compared to low-income non-participants, low-income program participants are less likely to meet fruit intake recommendations (p = 0.048) and that low-income SNAP and WIC participants are less likely to meet vegetable intake recommendations (p = 0.035). We also find lower rates of perceived stress among low-income school meal participant households compared to low-income nonparticipants (p = 0.039). Despite these mixed outcomes, participants broadly valued federal nutrition assistance programs, characterizing them as helpful or easy to use.
Conclusions
We find that federal nutrition assistance programs as a group were not sufficient to address food insecurity and stress or increase fruit and vegetable intake in the state of Vermont during the early months of the COVID-19 pandemic. Nonetheless, participants perceived benefits from participation in these programs. Optimizing the utility of nutrition assistance programs depends on critical examination of their functioning under conditions of great stress.
Association of Heart Rate with Left Ventricular and Left Atrial Functions in Hypertensive Patients with Normal Left Ventricular Ejection Fraction (A Speckle Tracking Study)
Authors: Emre Ender , Özderya Ahmet , Hancı Kaan , Turan Turhan , Tezen Ozan , Kalaycıoğlu Ezgi , Çetin Mustafa 
Published: June 2022

Abstract
Purpose
The structure and functions of the left heart cavities have important prognostic value in heart diseases and heart rate control is among the important treatment goals. In this study, we investigated the effects of heart rate on left heart structure and functions in hypertension patients with normal left ventricular systolic function.
Method
One-hundred-fifty-three patients were included in the study. The patients were divided into two groups according to their heart rate(70 beats/min and below and above 70 beats/min). Left ventricular and atrial strain analyses were performed in echocardiographic evaluation.
Results
Patients with a resting heart rate of 70 or less were named group-1(64.2±19), patients with a resting heart rate above 70 were named group-2(79.1±6.8). Left atrial max volüm (60.8±15.5 vs 52.9±16.3 p:0.007), left atrial min volüm(28.8±9.5 vs 22.6±7.9 p < 0.001), left atrial emptying fraction%(52.8±8.5 vs 56.1±8.5 p:0.035), left atrial expansion index (1.19±0.44 vs 1.36±0.47 p:0.044), GLSReLA(-1.3±0.38 vs -1.5±0.61 p:0.031), GLS(-19.3±3 vs. -18.2±2.7 p:0.07) showed difference between group-1 and group-2, respectively. In the multivariable regression analysis, beta-bloker(OR:0.291, 95% CI 0.105–0.810, p:0.018), mean high diastolic blood pressure(OR:1.054, 95% CI 1.009–1.101, p:0.018), left atrial min volume( OR:0.870, 95% Cl 0.809–0.938, p < 0.001), Sm(OR:10.6, 95% CI 1.1–104, p:0.041), left atrial expansion index(OR:0.870, 95% CI 0.809–0.930, p < 0.033) were determined as independent predictors of high resting heart rate.
Conclusions
Heart rate control emerges as an important goal in patients with hypertension and preserved left ventricular systolic function. Mortality and morbidity can also be improved by the positive effect of heart rate control on GLS.
Hypoxic pretreatment ADSCs exosome treatment promote spinal cord repair after spinal cord injury by delivery circ-Astn1 and activation microglia autophagy
Authors: Shao Minghao , Jin Mingming , Zhu Wei , Ma Xiaosheng , Lv Feizhou 
Published: June 2022

Abstract
Background: Exosome (Exo) secretion by hypoxic pretreatment adipose-derived mesenchymal stem cells (ADSCs) promotes spinal cord repair after spinal cord injury (SCI). But the mechanism remains unclear. Methods: High-throughput sequencing was used to investigate abnormal expression of circRNA in hypoxic pretreatment ADSCs exosome (HExo) and ADSCs exosome (Exo). The abnormal expression of mRNA in spinal cord tissues after Exo or HExo treatment were also analysis using high-throughput sequencing. Bioinformatics analysis and luciferase reporting analysis were used to clarify the interacted relationship among circRNA, miRNA and mRNA. BV2 cells were employ to analysis the apoptosis, inflammatory cytokines expression under OGD condition by using immunofluorescence, ELISA detection. SCI mice model were constructed and the therapeutic effect of Exo were detected using immunohistochemistry, immunofluorescence. Results: The result show that HExo have more therapeutic effect on spinal cord repair after SCI by activation of autophagy. High-throughput sequencing detection show that circ-Astn1 play a role in HExo mediated spinal cord repair after SCI. Downregulation circ-Astn1 decreased the therapeutic effect of HExo to spinal cord repair after SCI. The study also found that Atg7 play a role in HExo mediated spinal cord repair after SCI. Luciferase reporting analysis confirmed that both miR-138-5p and Atg7 were downstream targets of circ-Astn1. Downregulation of Atg7 or overexpression of miR-138-5p reversed the protective effect of circ-Astn1 to BV2 after exposure to OGD condition. Upregulation circ-Astn1 increased the therapeutic effect of Exo to spinal cord repair after SCI by activation autophagy. Conclusions: In conclusion, ADSC-Exos containing circ-Astn1 promotes spinal cord repair after SCI by targeting miR-138-5p/Atg7 pathway mediated autophagy.
HER3/CYLD axis mediates ovarian cancer cell resistance to chemotherapy via upregulation of ABCB1
Authors: Zhang Ye , Qiu Jian-Ge , Jia Xiao-Yu , Wang Wei , Liu Wen-Jing , Ding Chen , Liu Ling-Zhi , Wang Lin , Jiang Bing-Hua 
Published: June 2022

Abstract
Background: Ovarian cancer (OC) is the most pathogenic gynecological malignant tumor in the world. Due to the difficulty of early diagnosis, most of patients developed chemo-resistance and recurrence during/after the chemotherapy. Cylindromatosis (CYLD) is one member of lysine 63 deubiquitinases, and the regulatory mechanism of CYLD in mediating cisplatin (DDP) resistance need to be elucidated.Methods: Database analysis indicated that CYLD was associated with tumor development and prognosis; Patient tumor samples and clinicopathological characteristics were used to study the clinical significance of CYLD. Molecular biological methods were used to verify the function of CYLD in chemo-resistance with the parental and drug resistant cell lines (A2780/ A2780-DDP, OVCAR3/OVCAR3-DDP). Then flow cytometry analysis, co-IP and ChIP analysis were conducted to identify the upstream regulators and downstream effectors of CYLD; The drug combination method was used to investigate the drug synergistic effect of cisplatin combination with ABCB1 inhibitor or HER3 inhibitor. Animal models were further used to verify the role of CYLD in cancer chemo-resistance.Results: The expression levels of CYLD were downregulated in pan-cancer, especially in ovarian cancer, leading to acceleration of tumor progression and cisplatin resistance. CYLD knockdown in the parental cells was sufficient to induce DDP resistance through inhibiting cell apoptosis and promoting the drug efflux via inducing the expression of ABCB1. HER3 expression levels were much higher in the resistant cells, and HER3 was the upstream mediator for CYLD suppression through STAT3 signaling. Moreover, overexpression of CYLD in the resistant cells was sufficient to promote sensitivity to platinum-based treatment both in vitro and in vivo. ABCB1 was a functional downstream target of CYLD to regulate tumor growth and drug resistance both in vitro and in vivo.Conclusions: In this study, the results demonstrated that higher HER3 expression rendered CYLD suppression, consequently, increased cisplatin resistance and induced poor prognosis by inhibiting cell apoptosis and promoting the drug efflux in OC. Our findings showed that HER3/CYLD/ABCB1 is a new signaling pathway to regulate tumor growth and DDP resistance, which are potential new therapeutic targets to overcome DDP resistance.