bluuebunny/crossref_metadata_2025_split huggingface dataset:
Remodelling of tumour microenvironment by microwave ablation potentiates immunotherapy of AXL-specific CAR T cells against non-small cell lung cancer
Authors: Cao Bihui , Liu Manting , Wang Lu , Zhu Kangshun , Cai Mingyue , Chen Xiaopei , Feng Yunfei , Yang Shuo , Fu Shengyu , Zhi Cheng , Ye Xiaodie , Zhang Jian , Zhang Zhiru , Yang Xin , Zhao Ming , Wu Qingde , Xu Linfeng , Yang Lili , Lian Hui , Zhao Qi , Zhang Zhenfeng 
Published: October 2022

AbstractThe complex immunosuppressive tumour microenvironment (TME) and lack of tumour-specific targets hinder the application of chimeric antigen receptor (CAR) T cells in the treatment of solid tumours. Combining local treatment with CAR T cell immunotherapy may regulate the TME and enhance the killing potency of CAR T cells in solid tumours. Here, we show that AXL, which is highly expressed in non-small cell lung cancer (NSCLC) but not in normal tissues, might be a target for CAR T cell therapy. AXL-CAR T cells alone cause moderate tumour regression in subcutaneous and pulmonary metastatic lung cancer cell-derived xenograft models. Combination of microwave ablation (MWA) and AXL-CAR T cells have superior antitumour efficacy. MWA enhances the activation, infiltration, persistence and tumour suppressive properties of AXL-CAR T cells in AXL-positive NSCLC patient-derived xenograft tumours via TME remodelling. The combination therapy increases the mitochondrial oxidative metabolism of tumour-infiltrating CAR T cells. Combination treatment induces significant tumour suppression without observed toxicities in humanized immunocompetent mice. The synergistic therapeutic effect of MWA and AXL-CAR T cells may be valuable for NSCLC treatment.
Brainstem networks construct threat probability and prediction error from neuronal building blocks
Authors: Strickland Jasmin A. , McDannald Michael A. 
Published: October 2022

AbstractWhen faced with potential threat we must estimate its probability, respond advantageously, and leverage experience to update future estimates. Threat estimation is the proposed domain of the forebrain, while behaviour is elicited by the brainstem. Yet, the brainstem is also a source of prediction error, a learning signal to acquire and update threat estimates. Neuropixels probes allowed us to record single-unit activity across a 21-region brainstem axis in rats receiving probabilistic fear discrimination with foot shock outcome. Against a backdrop of diffuse behaviour signaling, a brainstem network with a dorsal hub signaled threat probability. Neuronal function remapping during the outcome period gave rise to brainstem networks signaling prediction error and shock on multiple timescales. The results reveal brainstem networks construct threat probability, behaviour, and prediction error signals from neuronal building blocks.
Reduced alcohol preference and intake after fecal transplant in patients with alcohol use disorder is transmissible to germ-free mice
Authors: Wolstenholme Jennifer T. , Saunders Justin M. , Smith Maren , Kang Jason D. , Hylemon Phillip B. , González-Maeso Javier , Fagan Andrew , Zhao Derrick , Sikaroodi Masoumeh , Herzog Jeremy , Shamsaddini Amirhossein , Peña-Rodríguez Marcela , Su Lianyong , Tai Yun-Ling , Zheng Jing , Cheng Po-Cheng , Sartor R. Balfour , Gillevet Patrick M. , Zhou Huiping , Bajaj Jasmohan S. 
Published: October 2022

AbstractAlcohol use disorder is a major cause of morbidity, which requires newer treatment approaches. We previously showed in a randomized clinical trial that alcohol craving and consumption reduces after fecal transplantation. Here, to determine if this could be transmitted through microbial transfer, germ-free male C57BL/6 mice received stool or sterile supernatants collected from the trial participants pre-/post-fecal transplant. We found that mice colonized with post-fecal transplant stool but not supernatants reduced ethanol acceptance, intake and preference versus pre-fecal transplant colonized mice. Microbial taxa that were higher in post-fecal transplant humans were also associated with lower murine alcohol intake and preference. A majority of the differentially expressed genes (immune response, inflammation, oxidative stress response, and epithelial cell proliferation) occurred in the intestine rather than the liver and prefrontal cortex. These findings suggest a potential for therapeutically targeting gut microbiota and the microbial-intestinal interface to alter gut-liver-brain axis and reduce alcohol consumption in humans.
ReSurveyGermany: Vegetation-plot time-series over the past hundred years in Germany
Authors: Jandt Ute , Bruelheide Helge , Berg Christian , Bernhardt-Römermann Markus , Blüml Volker , Bode Frank , Dengler Jürgen , Diekmann Martin , Dierschke Hartmut , Doerfler Inken , Döring Ute , Dullinger Stefan , Härdtle Werner , Haider Sylvia , Heinken Thilo , Horchler Peter , Jansen Florian , Kudernatsch Thomas , Kuhn Gisbert , Lindner Martin , Matesanz Silvia , Metze Katrin , Meyer Stefan , Müller Frank , Müller Norbert , Naaf Tobias , Peppler-Lisbach Cord , Poschlod Peter , Roscher Christiane , Rosenthal Gert , Rumpf Sabine B. , Schmidt Wolfgang , Schrautzer Joachim , Schwabe Angelika , Schwartze Peter , Sperle Thomas , Stanik Nils , Stroh Hans-Georg , Storm Christian , Voigt Winfried , von Heßberg Andreas , von Oheimb Goddert , Wagner Eva-Rosa , Wegener Uwe , Wesche Karsten , Wittig Burghard , Wulf Monika 
Published: October 2022

AbstractVegetation-plot resurvey data are a main source of information on terrestrial biodiversity change, with records reaching back more than one century. Although more and more data from re-sampled plots have been published, there is not yet a comprehensive open-access dataset available for analysis. Here, we compiled and harmonised vegetation-plot resurvey data from Germany covering almost 100 years. We show the distribution of the plot data in space, time and across habitat types of the European Nature Information System (EUNIS). In addition, we include metadata on geographic location, plot size and vegetation structure. The data allow temporal biodiversity change to be assessed at the community scale, reaching back further into the past than most comparable data yet available. They also enable tracking changes in the incidence and distribution of individual species across Germany. In summary, the data come at a level of detail that holds promise for broadening our understanding of the mechanisms and drivers behind plant diversity change over the last century.
Intrinsic connectivity within the affective salience network moderates adolescent susceptibility to negative and positive peer norms
Authors: Do Kathy T. , McCormick Ethan M. , Prinstein Mitchell J. , Lindquist Kristen A. , Telzer Eva H. 
Published: October 2022

AbstractNot all adolescents are equally susceptible to peer influence, and for some, peer influence exerts positive rather than negative effects. Using resting-state functional magnetic resonance imaging, the current study examined how intrinsic functional connectivity networks associated with processing social cognitive and affective stimuli predict adolescents’ (n = 87, ages 11–14 years) prosocial tendencies and risky behaviors in the context of positive and negative peer norms. We tested the moderating role of four candidate intrinsic brain networks—associated with mentalizing, cognitive control, motivational relevance, and affective salience—in peer influence susceptibility. Only intrinsic connectivity within the affective salience network significantly moderated the association between peer norms and adolescent behavior above and beyond the other networks. Adolescents with high intrinsic connectivity within the affective salience network reported greater prosocial tendencies in contexts with more positive peer norms but greater risk-taking behavior in contexts with more negative peer norms. In contrast, peer norms were not associated with adolescent behavior for individuals with low affective salience within-network intrinsic connectivity. The mentalizing network, cognitive control network, and motivational relevance network were not associated with individual differences in peer influence susceptibility. This study identifies key neural mechanisms underlying differential susceptibility to positive and negative peer influence in early adolescence, with a particular emphasis on the role of affective salience over traditional mentalizing, regulatory, and motivational processes.
Functional characterization of Mousterian tools from the Caucasus using comprehensive use-wear and residue analysis
Authors: Doronicheva E. V. , Golovanova L. V. , Kostina J. V. , Legkov S. A. , Poplevko G. N. , Revina E. I. , Rusakova O. Y. , Doronichev V. B. 
Published: October 2022

AbstractThe authors discuss functional characterization of Mousterian tools on the basis of their use-wear and residue analysis of five lithic tools from Mezmaiskaya cave and Saradj-Chuko grotto in the North Caucasus. The results represent the first comprehensive use-wear and residue analysis carried out on Mousterian stone artefacts in the Caucasus. This study unequivocally confirms the use of bitumen for hafting stone tools in two different Middle Paleolithic cultural contexts defined in the Caucasus, Eastern Micoquian and Zagros Mousterian.
Retinal circulation time/arm-to-retina time ratio in the fluorescein angiography to evaluate retina-specific hemodynamics
Authors: Mushiga Yasuaki , Nagai Norihiro , Ozawa Yoko 
Published: October 2022

AbstractTo evaluate dynamic circulatory flow in the retinal or choroidal circulatory disease, we retrospectively reviewed medical charts of 128 eyes of 128 patients who underwent video recorded fluorescein angiography (FA), at Department of Ophthalmology, St Luke’s International Hospital, between April and September 2020. Mean age was 64.2 ± 14.0 (range 37–93) years, and 87 (67.9%) patients were men. Mean arm-to-retina (AR) time was 16.2 ± 4.1 s, and mean retinal circulation (RC) time was 10.9 ± 3.3 s. Mean RC time/AR time (RC/AR) ratio was 0.69 ± 0.22. AR time was correlated with age, whereas RC time was not. RC time was positively correlated with AR time (R = 0.360, P = 0.017). Moreover, mean RC time was significantly longer, and RC/AR ratio was greater, in the retinal-disease group after adjusting for age and sex. Patients who had an RC/AR ratio ≥ 0.8 more frequently presented with retinal diseases. RC time and RC/AR ratio were negatively correlated with systolic blood pressure only in the retinal-disease group. Given that AR time reflects systemic hemodynamics, RC time, which reflects local circulatory fluency, was influenced by the systemic circulatory condition. Moreover, RC/AR ratio revealed that circulatory changes peculiar to the retina may also be involved in retinal-disease pathogenesis. This study may help elucidate the mechanisms of retinal diseases and assist in diagnosis, although further studies are required.
Association of KCNJ6 rs2070995 and methadone response for pain management in advanced cancer at end-of-life
Authors: Ozberk Deniz , Haywood Alison , Sutherland Heidi G. , Yu Chieh , Albury Cassie L. , Zunk Mathew , George Rani , Good Phillip , Griffiths Lyn R. , Hardy Janet , Haupt Larisa M. 
Published: October 2022

AbstractOpioids are the therapeutic agents of choice to manage moderate to severe pain in patients with advanced cancer, however the unpredictable inter-individual response to opioid therapy remains a challenge for clinicians. While studies are few, the KCNJ6 gene is a promising target for investigating genetic factors that contribute to pain and analgesia response. This is the first association study on polymorphisms in KCNJ6 and response to methadone for pain management in advanced cancer. Fifty-four adult patients with advanced cancer were recruited across two study sites in a prospective, open label, dose individualisation study. Significant associations have been previously shown for rs2070995 and opioid response in opioid substitution therapy for heroin addiction and studies in chronic pain, with mixed results seen in postoperative pain. In this study, no associations were shown for rs2070995 and methadone dose or pain score, consistent with other studies conducted in patients receiving opioids for pain in advanced cancer. There are many challenges in conducting studies in advanced cancer with significant attrition and small sample sizes, however it is hoped that the results of our study will contribute to the evidence base and allow for continued development of gene-drug dosing guidelines for clinicians.
Autotoxicity of Ambrosia artemisiifolia and Ambrosia trifida and its significance for the regulation of intraspecific populations density
Authors: Su Pei , Liu Xuelian , Wang Ruili , Liu Tong , Zhao Wenxuan , Sun Mingming , Wang Hanyue , Liu Yunxiao , Wu Qiang 
Published: October 2022

AbstractAmbrosia artemisiifolia and Ambrosia trifida are annual invasive plants that cause serious harm to agriculture, animal husbandry, and human health. Based on the important characteristic of high-density, cluster distribution of their populations, it is speculated that its autotoxins have an effect on density regulation. This study explored the regulation of autotoxicity on intraspecific density. We used water extracts from two plants to compare and verify the autotoxicity of seed germination, analysed the components of autotoxins. The results showed that A. artemisiifolia and A. trifida had significant autotoxicity, and the highest inhibition rates on seed germination were 27.21% and 77.94%, respectively; ultra-performance liquid chromatography tandem mass spectrometry analysis revealed that chlorogenic acid, caffeic acid, p-coumaric acid, and vanillin were the main autotoxins of the two plants. After the seeds were washed with water, the germination recovery rate of seeds increased with the increased of inhibition degree of autotoxins treatment. Therefore, this study verified the autotoxicity of A. artemisiifolia and A. trifida, which can promote and inhibit the seed germination of A. artemisiifolia and A. trifida to regulate intraspecific competition.
Determining the potential distribution of Oryctes monoceros and Oryctes rhinoceros by combining machine-learning with high-dimensional multidisciplinary environmental variables
Authors: Aidoo Owusu Fordjour , Ding Fangyu , Ma Tian , Jiang Dong , Wang Di , Hao Mengmeng , Tettey Elizabeth , Andoh-Mensah Sebastian , Ninsin Kodwo Dadzie , Borgemeister Christian 
Published: October 2022

AbstractThe African coconut beetle Oryctes monoceros and Asiatic rhinoceros beetle O. rhinoceros have been associated with economic losses to plantations worldwide. Despite the amount of effort put in determining the potential geographic extent of these pests, their environmental suitability maps have not yet been well established. Using MaxEnt model, the potential distribution of the pests has been defined on a global scale. The results show that large areas of the globe, important for production of palms, are suitable for and potentially susceptible to these pests. The main determinants for O. monoceros distribution were; temperature annual range, followed by land cover, and precipitation seasonality. The major determinants for O. rhinoceros were; temperature annual range, followed by precipitation of wettest month, and elevation. The area under the curve values of 0.976 and 0.975, and True skill statistic values of 0.90 and 0.88, were obtained for O. monoceros and O. rhinoceros, respectively. The global simulated areas for O. rhinoceros (1279.00 × 104 km2) were more than that of O. monoceros (610.72 × 104 km2). Our findings inform decision-making and the development of quarantine measures against the two most important pests of palms.
Adaptive filtering of microseismic data for monitoring a water-conducting fractured zone in a mine
Authors: Li Hongjiang , Han Liang , Dong Donglin 
Published: October 2022

AbstractWater-conducting fractured zones in a rock mass can cause problems in mining. Attempts have been made to monitor their development using microseismic signals. However, due to the lack of prior information, it is difficult to filter out mixed low-frequency interference with traditional denoising methods. In this work, the proposed adaptive filtering algorithm is applied after the wavelet packets are decomposed. It is based on a cross-correlation analysis. The algorithm takes a high-quality signal in the common source waveform as prior information and applies the corresponding correlation coefficients between subbands as a threshold. The algorithm was verified with simulations. The results show that low-frequency interference can be effectively suppressed by filtering. For single-frequency interference, the signal-to-noise ratio increased from − 10.18 to 13.97, and the root-mean-square error was 43.88. For multi-frequency interference, it increased from − 10.01 and − 2.63 to 13.50 and 7.99. The root-mean-square errors were 46.31 and 138.07. The narrower the main frequency band of the interference signal and the less the overlap of the main frequency band of the interference signal and the effective signal, the better the filtering effect. When the algorithm was applied to microseismic data collected in the field, the number of effective channels increased and the accuracy improved. The development of a water-conducting fractured zone in the field was consistent with the microseismic location obtained after interference was removed by the algorithm, which indicates that it is feasible to monitor a water-conducting fractured zone by analyzing microseismic waveforms with the adaptive filtering algorithm.
Adapted tensor decomposition and PCA based unsupervised feature extraction select more biologically reasonable differentially expressed genes than conventional methods
Authors: Taguchi Y-h. , Turki Turki 
Published: October 2022

AbstractTensor decomposition- and principal component analysis-based unsupervised feature extraction were proposed almost 5 and 10 years ago, respectively; although these methods have been successfully applied to a wide range of genome analyses, including drug repositioning, biomarker identification, and disease-causing genes’ identification, some fundamental problems have been identified: the number of genes identified was too small to assume that there were no false negatives, and the histogram of P values derived was not fully coincident with the null hypothesis that principal component and singular value vectors follow the Gaussian distribution. Optimizing the standard deviation such that the histogram of P values is as much as possible coincident with the null hypothesis results in an increase in the number and biological reliability of the selected genes. Our contribution was that we improved these methods so as to be able to select biologically more reasonable differentially expressed genes than the state of art methods that must empirically assume negative binomial distributions and dispersion relation, which is required for the selecting more expressed genes than less expressed ones, which can be achieved by the proposed methods that do not have to assume these.
The implications of globalization on COVID-19 vaccination in Europe
Authors: Lupu Dan , Tiganasu Ramona 
Published: October 2022

AbstractAlthough globalization has left its mark on economic dynamism, causing conditionalities among various aspects (market openness, production networks, technological and information developments, migratory flows, international cooperation, humanitarian support, etc.), the less pleasant side of it should not be omitted, i.e. the emergence of the framework for the faster diffusion of epidemiological diseases. Thus, with the onset of the SARS-CoV-2 virus, its widespread circulation is a serious challenge for the provision of efficient solutions to combat it, especially in countries with fragile health systems, poor institutional quality and lack of resources. In this paper we aim to investigate the implications of globalization on the COVID-19 vaccination of the population. The period under analysis is January 1, 2021–January 1, 2022, using montly data, and the object of our study are 48 European states. To capture the relationship between globalization and the vaccination rate, we applied regression models, including a number of factors that may influence the progress of vaccination. In order to test the robustness of the results, the two-stage least squares (2SLS) regressions was used. The regression models developed underlined that globalization impacts the degree of vaccination. More globalized economies are more competitive in COVID-19 management, and the significance of this effect comes from better interconnection in global markets and easier access to medical discoveries. At the same time, countries with a higher vaccination rate are associated with higher levels of development. Based on the results obtained, we proposed some policy recommendations to increase the propensity to vaccinate, ensure equity in the distribution of vaccines and provide financial support to developing countries.
Odds-ratio network for postoperative factors revealing differences in the 2-year longitudinal pattern of satisfaction between women and men after total knee arthroplasty
Authors: Gallo J. , Kriegova E. , Radvansky M. , Sloviak M. , Kudelka M. 
Published: October 2022

AbstractTimely and accurate assessments of the factors influencing satisfaction, a key indicator of success in primary total knee arthroplasty (TKA), may help improve TKA outcomes. Here we performed the longitudinal trend analysis of relation between satisfaction and 12 postoperative factors, which positively or negatively influence the patient satisfaction 2 years after TKA. In a real-world registry cohort (women/men: 1121/650), we showed similarities and differences between women and men in the contribution of postoperative factors to satisfaction 2 years after TKA as assessed by odds-ratio-similarity network. In men, the strongest negative factors were pain and complications, followed by mechanical problems. In women, the strongest negative factors were the pain and knee instability, followed by other mechanical problems, complications and low levels of sports activity. In both sexes, physical activity and the Knee Society Score (general and functional) influenced positively satisfaction; long-distance walking was associated with satisfaction only in women. A trend analysis revealed a reduction in the strength of satisfaction-related factors over 2 years of check-ups, particularly in women. Our study demonstrates that the key check-up for assessing the evolution of satisfaction in the 2 years after TKA was at 3 months in both sexes.