bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endopeptidase Cleavage of Anti-Glomerular Basement Membrane Antibodies in vivo in Severe Kidney Disease: An Open-Label Phase 2a Study
Authors: Uhlin Fredrik , Szpirt Wladimir , Kronbichler Andreas , Bruchfeld Annette , Soveri Inga , Rostaing Lionel , Daugas Eric , Lionet Arnaud , Kamar Nassim , Rafat Cédric , Mysliveček Marek , Tesař Vladimír , Fernström Anders , Kjellman Christian , Elfving Charlotte , McAdoo Stephen , Mölne Johan , Bajema Ingeborg , Sonesson Elisabeth , Segelmark Mårten 
Published: March 2022

Significance Statement

In vivo cleavage of IgG by an endopeptidase is a novel therapeutic strategy for anti-GBM disease. Despite plasma exchange, most patients become dependent on dialysis, especially those with AKI at diagnosis. In an encouraging pilot study, two thirds of 15 patients selected because of poor prognosis exhibited kidney survival at 6 months without major safety issues after receiving a single infusion of imlifidase. The drug has been used in patients who have undergone a transplant with multiple HLA antibodies. Our study supports further use of the drug in clinical situations in which IgG autoantibodies threaten vital organ function. However, randomized trials are necessary to confirm the findings.

Background
The prognosis for kidney survival is poor in patients presenting with circulating anti–glomerular basement membrane (GBM) antibodies and severe kidney injury. It is unknown if treatment with an endopeptidase that cleaves circulating and kidney bound IgG can alter the prognosis.

Methods
An investigator-driven phase 2a one-arm study (EudraCT 2016–004082–39) was performed in 17 hospitals in five European countries. A single dose of 0.25 mg/kg of imlifidase was given to 15 adults with circulating anti-GBM antibodies and an eGFR <15 ml/min per 1.73m2. All patients received standard treatment with cyclophosphamide and corticosteroids, but plasma exchange only if autoantibodies rebounded. The primary outcomes were safety and dialysis independency at 6 months.

Results
At inclusion, ten patients were dialysis dependent and the other five had eGFR levels between 7 and 14 ml/min per 1.73m2. The median age was 61 years (range 19–77), six were women, and six were also positive for anti–neutrophil cytoplasmic antibodies. Then 6 hours after imlifidase infusion, all patients had anti-GBM antibodies levels below the reference range of a prespecified assay. At 6 months 67% (ten out of 15) were dialysis independent. This is significantly higher compared with 18% (nine out of 50) in a historical control cohort (P<0.001, Fisher's exact test). Eight serious adverse events (including one death) were reported, none assessed as probably or possibly related to the study drug.

Conclusions
In this pilot study, the use of imlifidase was associated with a better outcome compared with earlier publications, without major safety issues, but the findings need to be confirmed in a randomized controlled trial.

Clinical Trial registration number: EUDRACT 2016–004082–39 https://www.clinicaltrialsregister.eu/ctr-search/trial/2007-001377-28/results
Pattern Recognition: Effectiveness of Teaching Boys Aged 15 the Press Headstand and Handstand
Authors: Siedykh Kyrylo , Ivashchenko Olha , Bartik Pavol , Veremeenko Viktoria 
Published: March 2022

Purpose. To determine the impact of exercise modes on the effectiveness of teaching boys aged 15 the press headstand and handstand.
Materials and methods. The study participants were 20 boys aged 15. The children and their parents were fully informed about all the features of the study and gave their consent to participate in the experiment. To solve the tasks set, the following research methods were used: study and analysis of scientific and methodological literature; pedagogical observation, timing of training tasks; pedagogical experiment, methods of mathematical statistics, discriminant analysis.
Results. The assumption was made about a significant influence of the modes of alternating exercise repetitions and the rest interval on the effectiveness of teaching boys aged 15 the press headstand and handstand. The study found that statistically significant differences in the number of repetitions are observed in performing all training tasks. The boys aged 15 who use the second mode (6 sets 2 times each with a rest interval of 60 s) need fewer repetitions to master series of motor tasks 1, 3, 4, 5, and 6. The boys aged 15 who use the first mode (6 sets 1 time each with a rest interval of 60 s) need fewer repetitions to master the second series of training tasks (exercises to master starting and ending positions).
Conclusions. The analysis of standardized, structure coefficients, and group centroids revealed that exercise modes significantly influence the process of teaching boys aged 15 the press headstand and handstand during physical education classes. The results of classification of the groups show that 100 % of the original grouped cases were classified correctly. The study found that the mode of 6 sets 2 times each with a rest interval of 60 s is more effective than the mode of 6 sets 1time with a rest interval of 60 s when teaching series of motor tasks 1, 3, 4, 5, and 6.
Reliability and validity of the Turkish version of the Subjective Health Complaints Inventory
Authors: KALAYCIOĞLU Oya , TOKUR KESGİN Makbule , DEMİR Gökçe 
Published: March 2022

Purpose: This study aims to evaluate the reliability and validity of the Turkish version of the Subjective Health Complaints (SHC) inventory, which can be used to monitor health complaints experienced by the hospital support staff.
Materials and Methods: In this methodological study, data were collected from 240 hospital support staff working in five different hospitals in Turkey. Participants were asked demographic questions and administered a Turkish translated SHC inventory. Construct validity was confirmed using exploratory and confirmatory factor analysis. Internal consistency was assessed by calculating the Cronbach’s alpha and split-half reliability coefficients.
Results: The Turkish SHC inventory with 29 items showed acceptable content validity, construct validity, and internal consistency reliability (Cronbach’s alpha = 0.905). Confirmatory factor analysis confirmed the original five-dimensional structure of the scale. Model fit indices implied a good model-data fit: the normed chi-square value of 1.488 (χ2 = 513.423, df=345); error of root mean square approximation of 0.045; comparative fit index of 0.939 and standardized root mean square residuals of 0.060.
Conclusion: The findings assert the reliability and validity of the Turkish SHC inventory, and recommend its use as a suitable tool for monitoring health complaints experienced by health service workers.
BIOLOGÍA REPRODUCTIVA Y AMENAZAS A LA FOCHA ANDINA Fulica ardesiaca (RALLIDAE) EN EL LAGO SAN PABLO (IMBABURA, ECUADOR)
Authors: Mena Patricio 
Published: March 2022

Durante 10 meses estudié la biología reproductiva de la Focha Andina Fulica ardesiaca en el lago San Pablo, norte de Ecuador. Hice observaciones semanales a una pareja focal y de manera esporádica a otras cuatro parejas entre octubre de 2017 y junio 2018. Las variables que estudié incluyeron: características del nido y de los huevos, periodo de incubación, éxito reproductivo y cambio de plumaje de los polluelos desde la eclosión hasta la dispersión. El periodo reproductivo de la pareja focal se extendió por 251 días entre el cortejo y el transporte de material para la construcción del nido hasta la dispersión. Los nidos fueron plataformas de material vegetal construidas en los bordes poco profundos del lago. El tamaño de la nidada de la pareja focal fue de seis huevos, y en las otras parejas entre 3-6 huevos. El periodo de incubación de la pareja focal fue de 20 días, y en otras dos parejas duró 21,7 ± 1,5 días. Los pichones nacieron cubiertos por plumones negros y blancos y en las alas, garganta y nuca tenían plumón rojizo anaranjado. Después de 22 días aparecieron las primeras plumas de sangre de las alas, y comenzaron cambios de color en algunas partes de la cabeza, cuerpo y cola, pasando por blanco, gris y café. A los 144 días, sus plumas eran de color café oscuro con algunas plumas parcialmente pigmentadas de negro pizarra y el escudo frontal adquirió su color distintivo. Estos resultados fueron consistentes con las observaciones en F.ardesiaca en Perú, aunque en algunos aspectos difirieron de otras especies del género.
APUNTES SOBRE LA ANIDACIÓN DEL PICOGRUESO DORADO Pheucticus chrysogaster (CARDINALIDAE) Y LA PARASITACIÓN POR PARTE DEL VAQUERO BRILLOSO Molothrus bonariensis (ICTERIDAE) AL SUROCCIDENTE DEL ECUADOR
Authors: Sanchez Nivicela Manuel Vicente , Sánchez-Muñoz Fredy 
Published: March 2022

Describimos el nido de Picogrueso Dorado Pheucticus chrysogaster chrysogaster en remanentes de vegetación de la región tumbesina en Piñas, provincia de El Oro, suroeste de Ecuador. Este nido fue parasitado por el Vaquero Brilloso Molothrus bonariensis. Los datos obtenidos extienden la temporalidad de reproducción de ambas especies a la época seca en esta región. También incrementamos la información de morfometría y descripción de los huevos y crías de ambas especies. Finalmente, analizamos brevemente los llamados de la hembra adulta y los dos pichones.
Forming environmental competence in future biology teachers through project tasks
Authors: Amantayeva Arailym , Karbayeva Sholpan , Childibayev Zhumadil , Turlybekova Gulzhazira , Issayev Gani , Stankevich Petr 
Published: March 2022

The impact of human economic activities on individual natural components or whole geosystems has caused a global environmental crisis and continues to change the surface of the planet beyond recognition. New biospheric thinking is required to overcome the existing threats. For that purpose, the new generation shall possess high ecological culture. The article aimed to examine the problem of formation and development of environmental competence of students based on project technology. The conducted research allowed students to independently identify environmental problems with the help of project technology. Practical development in the form of project tasks contributes to the formation of environmental competence in future biology teachers. The effectiveness of the proposed training technology is confirmed by the results of experimental work.

Keywords: environmental awareness; environmental competence; environmental education; didactic conditions; project tasks.
Developing women's business skills and entrepreneurial sustainability through informal entrepreneurship education
Authors: Essien Adeola Adesola , Adelekan Adedeji 
Published: March 2022

The role of women in business and how they contribute to national development cannot be overemphasised. There is a need to understand the role of informal education in enhancing the capacity of female managers and sustaining national growth. Hence, this study aims to establish the effect of informal entrepreneurial education on businesswomen management skills and entrepreneurial sustainability. The survey research design was employed in the study. 385 small and medium enterprises owners who are women were sampled. The regression analysis was employed in the study. The findings reveal that informal entrepreneurial education is a positive and significant driver of businesswomen’s management skills and entrepreneurial sustainability. it is recommended that SMEs owners, especially women, should aggressively increase their daily lives' learning capabilities. These will increase their capacity to improve their operations and discover new ways of doing things that will better enterprises' performance in the long run.

Keywords: Entrepreneurship; informal education; Skills; sustainability; women.
The Effect of Hydrolysis Time Using Microwave on Bioethanol Production from Sorghum Waste (Sorghum Bicolor L.)
Authors: Kolo Sefrinus Maria Dolfi 
Published: March 2022

In this study, the production of bioethanol from sorghum bagasse was carried out to understand the surface morphology of sorghum bagasse before and after hydrolysis, the effect of hydrolysis time using a microwave, and the concentration of inoculum on the ethanol content produced. A total of 10 grams of sorghum bagasse was suspended with 250 mL of 2% H2SO4 solution and then heated using a microwave at a temperature of 150ºC with variations in heating time of 20, 30,40, and 50 minutes. The heated hydrolyzate was analyzed for reducing sugar content using UV-Vis. The morphology of sorghum bagasse before and after hydrolysis was analyzed using SEM and quantitative analysis of fermented ethanol using a pycnometer and GC. The results showed that there were differences in the surface of sorghum bagasse before and after hydrolysis, the highest reducing sugar content was obtained at 30 minutes of hydrolysis, which was 30.4 g/L, and the highest concentration of Saccharomyces cerevisiae was 8% which resulted in 5.325% ethanol content using a pycnometer and 9.05% using GC. Keywords: Sorghum bagasse, Hydrolysis, Microwave, Bioethanol
Evaluating the Transport Performance of Novel-Shaped Proppant in Slickwater Fracturing with the Multiscale Modeling Framework
Authors: Zeng Junsheng , Li Heng , Li Sanbai , Zhang Dongxiao 
Published: March 2022

Summary
Recently, novel-shaped proppant, such as rod-shaped and x-shaped proppant, has been gradually used in hydraulic fracturing systems, which challenges the validity of previous transport laws for conventional spherical proppant. In this work, a multiscale modeling framework is proposed to solve this issue. We start from constructing particle-scale laws, including proppant settling, phase-slip, and effective slurry viscosity, based on a refined particle-resolved direct numerical simulation method, that is, the immersed boundary-computational fluid dynamics-discrete element method (IB-CFD-DEM). With this refined simulation method, particle-scale flow details are fully resolved, and accurate particle-scale laws can be reconstructed for novel-shaped proppant. These sub-scale laws are then applied to a field-scale simulation method, that is, the multiphase particle-in-cell (MP-PIC) method. Based on the proposed framework, transport performance of various types of proppant are investigated. Several numerical experiments demonstrate that proppant transport performance can be enhanced by 19 and 15% with x-shaped and rod-shaped proppant, respectively, compared to conventional spherical proppant under 5% inlet proppant concentration and enhanced by 16 and 10%, respectively, under 20% inlet proppant concentration. Moreover, related complicated flow mechanisms at different scales, such as the hindered effect and viscous gravity current effect, are fully discussed, which deepens our understanding of proppant transport and proppant placement.
Preparation of bilirubin standard solutions for assay calibration
Authors: Sist Paola , Tramer Federica , Urbani Ranieri , Bandiera Antonella , Passamonti Sabina 
Published: March 2022

Abstract
Bilirubin (BR) is the product of cellular heme catabolism and the major bile pigment in animal blood. It is an established biomarker of hemolysis and liver function. In the last decade, mild hyperbilirubinemia has been shown to be a biomarker of lower cardiovascular disease risk, attributed to its antioxidant and anti-inflammatory effects. To use bilirubin as a predictive biomarker, new powerful methods are currently being developed for its direct analysis in human blood. To harmonize the different methods, it is essential to use high-quality BR standard solutions for assay calibration. We present here a protocol for the preparation of stable standard solutions in the range of 10-9- 10-5 M BR at pH 7.4 that can facilitate a universal approach to assay calibration.
A Prospective Trial to Evaluate the Clinical Efficacy and Safety of Neoadjuvant Chemotherapy with Arsenic Trioxide and Carboplatin in Locally Advanced Cervical Cancer: A Study Protocol for Randomized Controlled Clinical
Authors: He ZeYang , Li HaiYing , Wan Xing , Li SiJin , Li DaoCheng , Liang ZhongZhe 
Published: March 2022

Abstract
Background：Cervical cancer is the fourth most common malignancy in women, which is threatening female reproductive tract health. Chemotherapy can be used for neoadjuvant therapy of locally advanced cervical cancer and postoperative adjuvant therapy for patients with high risk factors, so as to reduce the focus, sensitize radiotherapy and reduce recurrence. The current first-line treatment is paclitaxel combined with platinum. Many literature studies have found that As2O3 alone or in combination with platinum drugs have good efficacy in a variety of tumors both in vivo and in vitro. Moreover, our research group has verified that the efficacy of As2O3 combined with platinum drugs in the treatment of cervical cancer is not inferior to the traditional first-line regimen at the cellular and animal levels, and paclitaxel is more expensive than As2O3. Hence, we aim to evaluate the clinical efficacy and safety of neoadjuvant chemotherapy with As2O3 and carboplatin in locally advanced cervical cancer.Methods: Sixty participants in the IB2、IIA2 and IIB stages of cervical cancer will be recruited in this study. After excluding patients who didn't meet the criteria, they were randomly assigned to two groups in a 1:1 ratio. All patients underwent colposcopic biopsies to confirm the diagnosis and detailed clinical examinations. Eligible patients will receive either 2 cycles of paclitaxel (135-175 mg/m2) and carboplatin (dose 5 to 6 zones below the curve) or As2O3 (8 mg/m2) and carboplatin (5 to 6 zones below the curve) every 3 weeks.Patients were assessed for clinical efficacy after the second cycle of chemotherapy. Patients who had disease stable or disease progression at these time points will receive concurrent chemotherapy and radiation directly, while responders will receive PiverRutledge grade III radical hysterectomy, bilateral pelvic lymphadenectomy. Both groups of patients undergoing radical hysterectomy were given adjuvant therapy as per protocol-defined criteria. The efficacy and toxicity of two groups were evaluated according to WHO acute and subacute toxicity classification standards.Discussion: This is the first Single-centre, prospective, two-arm design, open-label randomized control trial that will evaluate the clinical efficacy and safety of neoadjuvant chemotherapy with As2O3 and carboplatin in locally advanced cervical cancer.Trial registration: ClinicalTrials.gov, ChiCTR1900023822. Registered on 13 June 2019
“Limbr, A Novel Personalized Mobile Application For Discogenic Chronic Low Back Pain: A Prospective Pilot Study”
Authors: Vad Vijay B. , Madrazo-Ibarra Antonio , Estrin Deborah , Carroll Kaitlin M , Vojta Deneen , Vad Amoli , Trapness Camilla , Pollack John P. 
Published: March 2022

Abstract
Background: Exercise has showed to reduce pain and improve function in patients with chronic lower back pain (CLBP), however, outcomes have been inconsistent. Limbr is a mobile application (app) developed to increase patient adherence to the exercise program, and enhance exercise’s technique for better results. Methods: Patients 18 to 65 years of age, with CLBP (more than 3 months) and evidence of lumbar disc pathology by magnetic resonance imaging (MRI) were enrolled. Patients’ symptomatology was prospectively evaluated at baseline and during 3 months of using the Limbr app with patient-reported outcome measures (PROMs) including: the Visual Analog Scale for pian (VAS), the Oswestry Disability Index (ODI), and the Your Activities of Daily Living (YADL) score. Patients’ compliance and satisfaction were evaluated at the end of the follow-up. Results: Seventy-five patients with CLBP were enrolled in the study. All patients had a significant improvement from baseline to final follow-up in all PROMs. Average VAS scores decreased from 5.17± 2.1 at baseline to 3.8±2.6 at final follow-up (P= 0.016), and ODI scores from 25.7±13.8 to 18.2±13.6 (P= 0.001). Patients showed a significant decrease in the number of pain medications taken during a week (P=0.001). Overall compliance with the app was greater than 52%, with almost 65% of the patients rating the overall experience as good or excellent. Conclusion: Limbr showed to be an effective tool in reducing low back pain symptomatology, as well as generating great compliance to an exercise program for patients with CLBP. Trial registration number: 2/2/2017 NCT03040310 (ClinicalTrials.gov)
Cell Density Detection Based on a Microfluidic Chip with Two Electrode Pairs
Authors: Wang Yongliang , Chen Danni , Guo Xiaoliang 
Published: March 2022

Abstract
Cell density detection is usually the counting of cells in certain volume of liquid, which is an important process in the field of biomedicine. The Coulter counting method has been an important method for biological cell detection and counting for decades. In this paper, a microfluidic chip based on two electrode pairs is designed, which uses the Coulter principle to detect the flow rate of uncertain volume liquid and count the cells, and then calculate the cell density by statistics. When the cell passes through the sensor channel formed by the electrode pair on the chip, the impedance will change between the electrodes. This phenomenon has been proved by experiments. The designed chip has the advantages of simple structure, small size and low manufacturing cost. The cell density detection method proposed in this article is of great significance to the research in the field of biological cell detection and development of related medical devices.
A Green Approach for Modification and Functionalization of Wool Fabric Using Bio- and Nano-Technologies
Authors: Ibrahim Nabil A. , Amin Hala A. , Abdel-Aziz Mohamed S. , Eid Basma 
Published: March 2022

Abstract
In the present work, we propose a green and sustainable strategy for eco-friendly surface modification of wool structure using biosynthesized kerationlytic proteases, from C4-ITA-EGY, Streptomyces harbinensis S11-ITA-EGY and Streptomyces carpaticus S33-ITA-EGY, followed by subsequent environmentally sound functionalization of the bio-treated substrates using ZnONPs, ZrO2NPs, ascorbic acid and vanillin, individually, to impart durable antibacterial and UV-protection properties. The changes in surface modification, and the extent of functionalization of the final products were characterized by SEM, EDX, antibacterial efficacy, UV-blocking ability, loss in weight, nitrogen content and durability to wash analysis. The obtained data reveals that, the developed green wool fabrics exhibit outstanding durable antibacterial activity and UV-blocking ability for fabricating multi-functional textile products can be utilized in a wide range of sustainable protective textiles, irrespective of the used post-finishing formulation ingredients. The results also show that both modification and functionalization processes are governed by type of enzyme and kind of active material respectively. Moreover, the biosynthesized kerationlytic proteases could be accessible used to remove protein-based stains like blood and egg.