bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autonomous vehicle impact on improving road network vulnerability
Authors: Obaid Mohammed , Török Árpád 
Published: June 2022

Abstract
Purpose
This study first presents a method to identify the parameters increasing road vulnerability on a macroscopic road network model. The second part exploresthe effect size difference of the analyzed attributes on network vulnerability through the implementation of different autonomous vehicles (AVs) penetrations and automation levels.

Methods
The road traffic network of Budapest, Hungary on PTV VISUM is studied by adopting a passenger car unit factor procedure to simulate the effect of AVs on road saturation. Five link parameters were used: length, distance from the centre, speed, number of lanes, and number of connectors. Network vulnerability was studied by simulating a combination of road elimination process with different passenger car unit values for AVs.

Results
The analysis found the number of road lanes is the most significant parameter, affecting the link criticality; followed by road length and distance from the centre. The analysis of four AV scenarios with different AV penetration and level of automation showed huge effect differences ranging from 3.50% for a simple AV automation level with low AV percentage to as large to 28.53% for a fully automated fleet.

Conclusions
AV implementation has proved efficient in reducing the amount of travel delays in the case of road failure. Finally, it was found that the number of lanes remained the most significant influencing parameter on travel delay. The main question is to discover the effect size difference of the analyzed attributes on network vulnerability through the implementation of different AVs penetrations and automation levels.
KAZN as a diagnostic marker in ovarian cancer: a comprehensive analysis based on microarray, mRNA-sequencing, and methylation data
Authors: Zhu Songling , Bao Hongxia , Zhang Meng-Chun , Liu Huidi , Wang Yao , Lin Caiji , Zhao Xingjuan , Liu Shu-Lin 
Published: June 2022

AbstractBackgroundOvarian cancer (OC) is among the deadliest malignancies in women and the lack of appropriate markers for early diagnosis leads to poor prognosis in most cases. Previous studies have shown thatKAZNis involved in multiple biological processes during development, such as cell proliferation, differentiation, and apoptosis, so defects or aberrant expression ofKAZNmight cause queer cell behaviors such as malignancy. Here we evaluated theKAZNexpression and methylation levels for possible use as an early diagnosis marker for OC.MethodsWe used data from Gene Expression Omnibus (GEO) microarrays, The Cancer Genome Atlas (TCGA), and Clinical Proteomic Tumor Analysis Consortium (CPTAC) to investigate the correlations betweenKAZNexpression and clinical characteristics of OC by comparing methylation levels of normal and OC samples. The relationships among differentially methylated sites in theKAZNgene, correspondingKAZNmRNA expression levels and prognosis were analyzed.ResultsKAZNwas up-regulated in ovarian epithelial tumors and the expression ofKAZNwas correlated with the patients’ survival time.KAZNCpG site cg17657618 was positively correlated with the expression of mRNA and the methylation levels were significantly differential between the group of stage “I and II” and the group of stage “III and IV”. This study also presents a new method to classify tumor and normal tissue in OC using DNA methylation pattern in theKAZNgene body region.ConclusionsKAZNwas involved in ovarian cancer pathogenesis. Our results demonstrate a new direction for ovarian cancer research and provide a potential diagnostic biomarker as well as a novel therapeutic target for clinical application.
Resident and staff perceptions of an activity- and recovery-based intervention in supported housing for people with severe mental illness – a longitudinal pilot study
Authors: Eklund Mona , Tjörnstrand Carina 
Published: June 2022

Abstract
Background
People with severe mental illness who reside in supported housing (SH) and need a high level of assistance are at risk of an everyday life with little meaning and low community participation. Interventions to counteract that seem warranted, which was the rationale for this study. The aim was to investigate how residents and staff perceived an intervention designed to enhance meaningful everyday activity and personal recovery.

Methods
The intervention, termed Active in My Home (AiMH), was led by an occupational therapist. It consisted of five individual and three group sessions, and AiMH staff acted as supporters. Twenty-nine AiMH participants and 43 staff members were included in this un-controlled study with three measurement points – before (T1), at completion (T2), and 6–9 months after completion of AiMH (T3). The data collection was based on self-report questionnaires addressing perceptions of satisfaction, meaningfulness, and recovery-oriented support.

Results
The residents’ satisfaction with the SH per se was rated high (at 75% of the maximum score) and did not change over the study period from T1 to T3 (p = 0.544); nor did the participants’ perceived recovery-oriented support from the AiMH supporter (p = 0.235). Satisfaction with AiMH was rated by both participants and staff at T2. Their scores differed regarding general satisfaction (p = 0.008), staff scoring higher, but no differences were found regarding satisfaction with group sessions, individual sessions, or support of activity (p-values 0.062–0.836). The staff rated the SH unit’s provision of meaningful activities higher than the AIMH participants at T2 (p = 0.029) but not at T1 (p = 0.226) or T3 (p = 0.499).

Conclusion
This study has offered some glimpses of how AiMH participants and staff perceived the AiMH intervention. It has also generated some ideas for better support for meaningful activity and recovery-oriented support in SH for people with mental illness, such as assisting SH residents in identifying activity opportunities and making activity choices when providing support for meaningful activity in the SH context.

Trial registration
Registered at ClinicalTrials.gov ID: NCT05157854.
Effect of prone position in patients with acute respiratory distress syndrome supported by venovenous extracorporeal membrane oxygenation: a retrospective cohort study
Authors: Chen Ziying , Li Min , Gu Sichao , Huang Xu , Xia Jingen , Ye Qinghua , Zheng Jiangnan , Zhan Qingyuan , Wang Chen 
Published: June 2022

Abstract
Background
The application of prone position (PP) in acute respiratory distress syndrome (ARDS) supported by venovenous extracorporeal membrane oxygenation (VV-ECMO) is controversial.

Objectives
To evaluate the safety and efficacy of application of PP during VV-ECMO in patients with ARDS.

Methods
This was a single-center, retrospective study of patients who met the Berlin definition of ARDS, and were supported with VV-ECMO. We divided the patients into two groups. The prone group included patients who were supported by VV-ECMO, and experienced at least one period of PP, while those without PP during VV-ECMO were defined as the supine group. Propensity score matching (PSM) at a ratio of 1:1 was introduced to minimize potential confounders. The primary outcomes were the complications of PP and the change of arterial oxygen pressure/fraction of the inspiration (PaO2/FiO2) ratio after PP. The secondary outcomes were hospital survival, ICU survival, and ECMO weaning rate.

Results
From April 2013 to October 2020, a total of 91 patients met the diagnostic criteria of ARDS who were supported with ECMO. 38 patients (41.8%) received at least one period of PP during ECMO, while 53 patients (58.2%) were maintained in supine position during ECMO. 22 minor complications were reported in the prone group and major complications were not found. The other ECMO-related complications were similar between two groups. The PaO2/FiO2 ratio significantly improved after PP compared with before (174.50 (132.40–228.25) mmHg vs. 158.00 (122.93–210.33) mmHg, p < 0.001). PSM selected 25 pairs of patients with similar characteristics. Hospital survival or ICU survival did not differ between the two groups (40% vs. 28%, p = 0.370; 40% vs. 32%, p = 0.556). Significant difference of ECMO weaning rate between two groups was not found (56% vs. 32%, p = 0.087).

Conclusions
PP during VV-ECMO was safe and could improve oxygenation. A large-scale and well-designed RCT is needed in the future.
Cross-cultural adaptation and validation of the Thai version of the functional index for hand osteoarthritis (FIHOA)
Authors: Suwannaphisit Sitthiphong , Tangtrakulwanich Boonsin , Suwanno Porames , Sinnathakorn Nitiphoom , Maheu Emmanuel 
Published: June 2022

Abstract
Background
The Functional Index for Hand Osteoarthritis (FIHOA) is a simple tool to assess functional impairment for hand OA patients. The purpose of this study was to translate the FIHOA into the Thai language, and validate it in Thai hand OA patients.

Methods
The FIHOA was translated into Thai (T-FIHOA) according to the principles of
cross-cultural adaptation and administered to 102 hand OA patients recruited between September 2020 and July 2021 together with the modified Health Assessment Questionnaire (mHAQ), Disabilities of the Arm, Shoulder and Hand (DASH), and visual analogue scale (VAS) for hand pain. Spearman’s correlation coefficient and intraclass correlation coefficient (ICC) were used to check the test-retest reliability of each item and the total scores in the translated questionnaire. The internal consistency reliability was evaluated using Cronbach’s alpha. The external construct validity was assessed using correlations between the T-FIHOA, mHAQ, DASH and hand pain VAS.

Results
The T-FIHOA had a unidimensional structure. The ICC was > 0.9 and the Cronbach’s alpha of 0.92 indicated excellent reliability and internal consistency, respectively. The external validity tests indicated moderate correlation with the hand pain VAS (r = 0.37, P <  0.01) and moderate to strong correlations with the mHAQ (r = 0.63, P <  0.01), and DASH score (r = 0.52, P <  0.01). The T-FIHOA had the highest effect size (ES) and standardized response mean (SRM) (− 0.37 and − 0.58 respectively) among all questionnaires except for the VAS when assessing changes between baseline and week 4.

Conclusions
The T-FIHOA is a good and reliable assessment tool freely available for practitioners/researchers to evaluate functional impairment in Thai hand OA patients.
Knowledge, and practice of cervical cancer prevention and associated factors among commercial sex workers in Shashemene Town, West Arsi, Oromia Region, Ethiopia
Authors: Argaw Muche , Embiale Aynamaw , Amare Belay 
Published: June 2022

Abstract
Background
The distribution of deaths and morbidities related to cervical cancer is disproportionally higher in low- and middle-income countries. In Ethiopia, there is a limited study on cervical cancer prevalence on Sex Workers, but a study conducted in Central America risk of developing HPV infection in sex workers is 2.5 times more than the general population. But a study conducted in the general population in Ethiopia reports that the incidence and mortality due to cervical cancer in Ethiopia is 26.4 and 18.4/100,000 respectively. However, there is limited data on knowledge and practice of cervical cancer prevention among sexual sex workers in Ethiopia.

Methods
A health institution-based cross-sectional study design was used to investigate the knowledge and practice of cervical cancer prevention and its associated factors among 405 women of commercial sex workers with a systematic random sampling technique and with an interviewr administered. Multivariate logistic regression was used to identify associated factors of knowledge and practice towards cervical cancer prevention with a p value < 0.05.

Result
In this study, three hundred eighty-five participants were included making a response rate of 95%. About half (50.1%) of respondents have knowledge regarding to cervical cancer, screening, and its prevention, and 20.3% of respondents were practiced cervical cancer screening. Having a history of use of combined oral contraceptives, AOR 2.190, (95% CI 1.374–3.492) and having a history of sexually transmitted infection, AOR 1.861, (95% CI 1.092–3.172). were significantly associated factors with knowledge of cervical cancer prevention. Regarding their uptake of cervical cancer screening, the level of knowledge was a significant factor, AOR 17.216 (95% CI 7.092–41.793).

Conclusions
The study participants have an average knowledge of cervical cancer and its prevention and their practice was low as other women. Curtailing cervical cancer; through increasing their knowledge by integrating sexual and reproductive health services to cervical cancer screening clinics and equipping them with audiovisual materials that will increase their knowledge that end up with good uptake.
Analysis of TMIE gene mutations including the first large deletion of exon 1 with autosomal recessive non-syndromic deafness
Authors: Rayat Sima , Farhadi Mohammad , Emamdjomeh Hessamaldin , Morovvati Saeid , Falah Masoumeh 
Published: June 2022

Abstract
Background
Transmembrane inner ear (TMIE) protein is an essential component of the mechanotransduction complex. In collaboration with other components, TMIE aids the maintenance and function of the sensory hair cells. Autosomal recessive deafness-6 (DFNB6) is caused by mutated TMIE, a gene in the high genetic heterogeneity spectrum of deafness. Hearing loss has a significant impact on the global economy and the quality of life of affected persons, their families, and society. Here, three unrelated families with TMIE variants are presented. All three cases were found while studying the genetic causes of an Iranian cohort of subjects with cochlear implants.

Methods
Whole exome sequencing was performed to find possible genetic etiology in probands of families after a comprehensive medical evaluation for hearing loss. Co-segregation analysis in probands and other family members was performed by Sanger sequencing. The variants were interpreted per the American College of Medical Genetics and Genomics guidelines.

Results
Three different variants associated with TMIE were confirmed as reasons for autosomal recessive non-syndromic deafness. The first novel ~ 10-kb deletion surrounding exon 1 of TMIE along with two previously reported variants co-segregated with families including a frameshift variant c.122_125dup (p.Pro43fs) and a missense variant c.250 C > T; p.(Arg84Trp) in exons 2, and 3, respectively.

Conclusion
This study increases the mutational spectrum of the TMIE gene and highlights the importance of the large deletion of this gene as a reason for hearing loss. Moreover, an efficient and simple multiplex PCR assay was developed to determine the exact breakpoints of the TMIE deletion.
Astaxanthin and eicosapentaenoic acid production by S4, a new mutant strain of Nannochloropsis gaditana
Authors: Cecchin Michela , Cazzaniga Stefano , Martini Flavio , Paltrinieri Stefania , Bossi Simone , Maffei Massimo E. , Ballottari Matteo 
Published: June 2022

Abstract
Background
Astaxanthin is a ketocarotenoid with high antioxidant power used in different fields as healthcare, food/feed supplementation and as pigmenting agent in aquaculture. Primary producers of astaxanthin are some species of microalgae, unicellular photosynthetic organisms, as Haematococcus lacustris. Astaxanthin production by cultivation of Haematococcus lacustris is costly due to low biomass productivity, high risk of contamination and the requirement of downstream extraction processes, causing an extremely high price on the market. Some microalgae species are also primary producers of omega-3 fatty acids, essential nutrients for humans, being related to cardiovascular wellness, and required for visual and cognitive development. One of the main well-known producers of omega-3 fatty eicosapentaenoic acid (EPA) is the marine microalga Nannochloropsis gaditana (named also Microchloropsis gaditana): this species has been already approved by the Food and Drug Administration (FDA) for human consumption and it is characterized by a fast grow phenotype.

Results
Here we obtained by chemical mutagenesis a Nannochloropsis gaditana mutant strain, called S4, characterized by increased carotenoid to chlorophyll ratio. S4 strain showed improved photosynthetic activity, increased lipid productivity and increased ketocarotenoids accumulation, producing not only canthaxanthin but also astaxanthin, usually found only in traces in the WT strain. Ketocarotenoids produced in S4 strain were extractible in different organic solvents, with the highest efficiency observed upon microwaves pre-treatment followed by methanol extraction. By cultivation of S4 strain at different irradiances it was possible to produce up to 1.3 and 5.2 mgL−1 day−1 of ketocarotenoids and EPA respectively, in a single cultivation phase, even in absence of stressing conditions. Genome sequencing of S4 strain allowed to identify 199 single nucleotide polymorphisms (SNP): among the mutated genes, mutations in a carotenoid oxygenase gene and in a glutamate synthase gene could explain the different carotenoids content and the lower chlorophylls content, respectively.

Conclusions
By chemical mutagenesis and selection of strain with increased carotenoids to chlorophyll ratio it was possible to isolate a new Nannochloropsis gaditana strain, called S4 strain, characterized by increased lipids and ketocarotenoids accumulation. S4 strain can thus be considered as novel platform for ketocarotenoids and EPA production for different industrial applications.
The private sector market for malaria rapid diagnostic tests in Nigeria: results of the 2018 market survey
Authors: Edwards Hannah M. , Sarwar Rubaiyath , Mahmud Parvez , Emmanuel Shekarau , Maxwell Kolawole , Tibenderana James K. 
Published: June 2022

Abstract
Background
To avoid misuse of anti-malarials, correct diagnosis of fever prior to drug prescription is essential. Presumptive treatment in the private healthcare sector is a concern in Nigeria, where availability of affordable artemisinin-based combination therapy (ACT) is high following the implementation of subsidy schemes from 2010 to 2017. Similar subsidies have not, however, been implemented for malaria rapid diagnostic tests (RDTs). A market survey in 2018 predominantly designed to assess the ACT market in the private sector also collected data related to RDTs, results of which are presented herein.

Methods
A 2018 market survey consisted of (i) an outlet survey targeting private pharmacies and Proprietary and Patent Medicine Vendors (PPMVs) across different regions of Nigeria to assess supply-side market factors related to availability of RDTs (defined as having stock available for purchase at the time of the survey) and (ii) a household survey to determine demand-side factors related to knowledge of RDTs, healthcare-seeking practices and affordability.

Results
Availability of RDTs at the time of the survey was low in both outlet types and significantly lower in PPMVs (22.1%, 95% CI) among pharmacies versus (13.6%, 95% CI) among PPMVs (p < 0.01). Reasons for not restocking RDTs included low demand and no supply. The majority of households diagnose malaria based on experience, while one-third would visit a PPMV or pharmacy. Half of households had heard of RDTs (48.4%) and 38.6% thought they were affordable.

Conclusions
Low availability of RDTs among PPMVs and pharmacies may be attributed to lack of demand, supply-side issues and cost. Increasing household knowledge of RDTs may aid increasing demand, while subsidized RDTs may address supply and price issues. Addressing the deficit in RDT provision is important for targeting of ACT medicines.
Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells
Authors: Perez Jose E. , Bajaber Bashaer , Alsharif Nouf , Martínez-Banderas Aldo I. , Patel Niketan , Sharip Ainur , Di Fabrizio Enzo , Merzaban Jasmeen , Kosel Jürgen 
Published: June 2022

Abstract
Background
Nanotopographical cues play a critical role as drivers of mesenchymal stem cell differentiation. Nanowire scaffolds, in this regard, provide unique and adaptable nanostructured surfaces with focal points for adhesion and with elastic properties determined by nanowire stiffness.

Results
We show that a scaffold of nanowires, which are remotely actuated by a magnetic field, mechanically stimulates mesenchymal stem cells. Osteopontin, a marker of osteogenesis onset, was expressed after cells were cultured for 1 week on top of the scaffold. Applying a magnetic field significantly boosted differentiation due to mechanical stimulation of the cells by the active deflection of the nanowire tips. The onset of differentiation was reduced to 2 days of culture based on the upregulation of several osteogenesis markers. Moreover, this was observed in the absence of any external differentiation factors.

Conclusions
The magneto-mechanically modulated nanosurface enhanced the osteogenic differentiation capabilities of mesenchymal stem cells, and it provides a customizable tool for stem cell research and tissue engineering.

Graphical Abstract
A knowledge implementation model in health system management based on the PARIHS model
Authors: Roohi Ghanbar , Jahani Mohammad Ali , Farhadi Zeynab , Mahmoudi Ghahraman 
Published: June 2022

AbstractBackgroundThe gap between knowledge and practice, along with postponing or not implementing research findings in practice and policy-making, is one of the reasons for low-quality services. Hence, this study aimed at presenting a model of knowledge implementation in health system management in Iran.MethodsThe present two-phase study was first performed qualitatively using a directive content analysis approach based on the Promoting Action on Research Implementation in Health Services (PARIHS) model. The researchers extracted the barriers and facilitators by conducting semi-structured individual interviews. Then, in a three-stage Delphi study, 25 health experts determined the barrier removal strategies. Data were analysed using MAXQDA10 software.Results The content analysis of the interviews led to the emergence of 1212 codes under three categories of evidence, context and facilitation. The findings indicate that health managers make fewer decisions based on research findings. Instead, they make decisions regarding the experiences of service providers and organization data. In addition to the subcategories in the PARIHS model, the researchers extracted political, social and administrative factors under the context category. The relationships between the features of evidence, context, facilitation, barriers and strategies were presented in the final model.Conclusion The presented model comprehensively emphasizes the evidence resources, context preparation, and facilitation of the knowledge implementation process.