bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soft tissue stabilization of the hinge position in medial closed wedge distal femoral osteotomy: an anatomical study
Authors: Oda Taiga , Maeyama Akira , Yoshimura Ichiro , Ishimatsu Tetsuro , Miyazaki Kotaro , Tachibana Katsuro , Yoshimitsu Kengo , Yamamoto Takuaki 
Published: October 2022

Abstract
Background: Soft tissue has an important role in stabilizing the hinge point of medial closed wedge distal femoral osteotomy (MCWDFO). However, there are conflicting data on the soft tissue anatomy around the hinge point of MCWDFO and, therefore, further anatomical data are needed. The purposes of the study were to: 1) anatomically analyze the soft tissue around the hinge point of MCWDFO; 2) radiologically define the appropriate hinge point to prevent an unstable hinge fracture based on the result of the anatomical analysis; and 3) histologically analyze the soft tissue based on the result of the anatomical analysis.
Methods:
In 20 cadaveric knees, the capsule attachment of the distal lateral side of the femur was marked with a radiopaque ball bearing. A digital planning tool was used to calculate the area of the marked capsule attachment around the ideal hinge point of MCWDFO on radiographs. The soft tissue around the hinge point was histologically examined and the periosteal thickness was measured and visualized graphically. The graph and radiograph were overlayed using image editing software, and the appropriate hinge position was determined based on the periosteal thickness.
Results:
As a result, the periosteal thickness of the distal lateral femur tended to rapidly decrease from the metaphyseal region toward the diaphyseal region. The overlayed graph and radiograph revealed that the periosteal thickness changed in the region corresponding to the apex of the turning point of the femoral metaphysis in all cases.
Conclusions:
In conclusion, the periosteum might support the hinge of MCWDFO within the area surrounded by the apex of the turning point of the femoral metaphysis and the upper border of the posterior part of the lateral femoral condyle.
Rice growth stages modulate rhizosphere bacteria and archaea co-occurrence and sensitivity to long-term inorganic fertilization
Authors: Dondjou Donald Tchouomo , Diedhiou Abdala Gamby , Mbodj Daouda , Mofini Marie-Thérèse , Pignoly Sarah , Ndiaye Cheikh , Diedhiou Issa , Assigbetse Komi , Manneh Baboucarr , Laplaze Laurent , Kane Aboubacry 
Published: October 2022

Abstract
Rhizosphere microbial communities are important components of the soil-plant-atmosphere continuum in paddy field ecosystems where they contribute to nutrient cycling and rice productivity. However, the rhizosphere microbial sensitivity to anthropic soil disturbance across plant growth stages remains little investigated. Here, we tracked the effects of long-term (> 25 years) N and NPK-fertilization on bacterial and archaeal community inhabiting the rice rhizosphere at three growth stages (tillering, panicle initiation and booting). Our results reveal that the effect of long-term inorganic fertilization on rhizosphere microbial communities varied with growth stage and that the bacterial and archaeal community differed in their response to N and NPK-fertilization. The microbial communities inhabiting the rice rhizosphere at the panicle initiation appear to be more sensitive to long-term inorganic fertilization than those at the tillering and booting stage. However, the effect of growth stage on microbial sensitivity to long-term inorganic fertilization was more strongly pronounced for bacterial than archaeal community. Furthermore, our results reveal dynamics of bacteria and archaea co-occurrence patterns in the rice rhizosphere, with differentiated bacterial and archaeal pivotal roles in the microbial inter-kingdom networks across growth stages. Hence, our study brings new insights on rhizosphere bacteria and archaea co-occurrence and sensitivity to long-term inorganic fertilization across growth stages in field-grown rice. By identifying one of the critical rice growth stages during which rhizosphere microbial communities are highly sensitive to inorganic fertilization, our results open new avenues for developing appropriate strategies in microbiome engineering to mitigate biotic and abiotic stress and improve crop yields.
Projection of Temperature Extremes of Egypt Using CMIP6 Shared Socioeconomic Pathways
Authors: Hamed Mohammed Magdy , Salehie Obaidullah , Nashwan Mohamed Salem , Shahid Shamsuddin 
Published: October 2022

Abstract
Global warming has amplified the frequency and other properties of temperature extremes, especially in hot dry countries, and significantly affected the environment. Egypt is one of the hot desert climate regions that are more susceptible to climate change and associated hazards. This study attempted to project the changes in temperature extremes for three Shared Socioeconomic Pathways (SSPs), namely SSP1-2.6, SSP2-4.5, and SSP5-8.5 and two future periods (early future: 2020–2059, and late future: 2060–2099) by using annual maximum (Tmax) and minimum temperature (Tmin) of general circulation model (GCMs) of Coupled Model Inter-comparison Project phase 6 (CMIP6). The findings showed that most temperature extremes indices would increase especially by the end of the century. In the late future, the change in the mean minimum temperature (4.3°C) was projected to be higher than the mean maximum temperature (3.7°C). The number of hot days would increase by more than 80 days in the late future over the south and southeast of the country. Contrarily, the cold night temperatures would decrease by -0.95 to -3.0°C for different SSPs. The study indicates that the country is likely to experience an additional hotter climate rather than a colder one.
Early experience in minimally invasive esophagectomy for treatment of thoracic esophageal cancer
Authors: Achim Florin , Constantin Adrian , Mossen Ahmed , Rosianu Cristian , Constantinoiu Silviu , Predescu Dragos 
Published: October 2022

Abstract
Introduction: Minimally invasive esophagectomy (MIE) has gained popularity in the surgical treatment of esophageal cancer over the past three decades. By introducing minimally invasive techniques, esophageal oncological surgery has advanced in terms of safety, radicality, functional preservation and quality of life post-esophagectomy of the patients.
Material and Method: We present the experience of the Center of Excellence in Esophageal Surgery at Sf. Maria Clinical Hospital regarding total (MIE) through total 3D HD thoraco-laparoscopic modified McKeown triple approach. In between January 2015 - December 2021, 24 patients (M) were treated for thoracic esophageal cancer. The average age of patients was 57 years. Most of the esophageal tumors were midthoracic (62.5%), 83.3% were squamous carcinomas and half were moderately differentiated. Most of the esophageal tumors were locally advanced tumors (cT3N1 – 58.3%), 18 patients were staged III. 70.8% of patients received neoadjuvant chemo radiotherapy.
Results: Thoracoscopic esophageal mobilizations were successful in all 24 patients, no conversion to thoracotomy. Laparoscopic gastric mobilizations were successful in all 24 patients, conversion to open technique was performed in 7 patients for creation extracoporeally of the gastric conduit through an epigastric minilaparotomy. The esophageal substitute was represented by the gastric conduit. The intraoperative blood loss was minimal. The average duration of surgical interventions was 360 minutes. All the surgical interventions were considered with curative intention (R0). The rate of intraoperative complications was 20.8%. The rate of early postoperative complications was 66%. Distribution of postoperative complications according to the Clavien-Dindo Classification (0-33.3%, 1-16.6%, 2-4.1%, 3a-12.5%, 3b-8.3%, 4a-0%, 4b-8.3%, 5-16.6%) highlighted a rate of 33.3% of major complications. The advantages of MIE observed in the studied group in the postoperative period were: reduced postoperative pain, improved comfort and early mobilization of the patients.
Conclusions: The early outcomes of using the minimally invasive approach in the treatment of esophageal cancer in our clinic include the reduction of perioperative morbidity, the duration of hospitalization and a faster recovery.
Online Parent Training for The Initial Management of ADHD referrals (OPTIMA): The protocol for a randomised controlled trial of a digital parenting intervention implemented to support parents and children on a treatment waitlist.
Authors: Kostyrka-Allchorne Kasia , Ballard Claire , Byford Sarah , Cortese Samuele , Daley David , Downs Johnny , French Blandine , Glazebrook Cristine , Goldsmith Kimberley , Hall Charlotte L , Hedstrom Ellen , Kovshoff Hanna , Kreppner Jana , Lean Nancy , Sayal Kapil , Shearer James , Simonoff Emily , Thompson Margaret , Sonuga-Barke Edmund 
Published: October 2022

Abstract
Background
Children referred for attention-deficit hyperactivity disorder (ADHD) often present with a broader pattern of conduct problems including oppositionality and defiance. This combination can be extremely stressful to parents, lower parents’ self-esteem, and negatively impact family life. The National Institute for Health and Care Excellence (NICE) recommends that families receive support as soon as possible after their referral. However, as clinical services are overstretched, and traditional in-person parenting intervention programmes are expensive, families often must waittimes a long time prior to receiving this vital input. To address this, we have created a digital parenting programme called STEPS. It is delivered as a mobile phone app providing a set of tools and resources that can be easily accessed at parents’ convenience. This study aims to evaluate the clinical- and cost-effectiveness of STEPS in supporting parents of children with high levels of hyperactivity/impulsivity, inattention, and conduct problems, who are waiting to be assessed by specialist children’s clinical services.
Methods
Online Parent Training for The Initial Management of ADHD referrals (OPTIMA) is a two-arm superiority parallel randomised controlled trial with an internal pilot study. We aim to recruit 352 parents and their children, who have been accepted onto a waitlist in Child and Adolescent Mental Health Services or similar child health services. Parents who consent will be randomised 1:1 to either the STEPS or wait-as-usual (WAU) group. The trial will be conducted remotely (online and telephone) with measures taken at baseline, three-, six -, nine-, and 12- months post-randomisation. The primary objective is to evaluate whether STEPS reduces the severity of children’s oppositional and defiant behaviour, as rated by parents, measured at three months post-randomisation compared to WAU.
Discussion
Digital solutions, such as mobile phone apps, have potential for delivering psychological support for parents of children with clinical-level needs in a timely and inexpensive manner. This trial will provide data on the clinical- and cost-effectiveness of the STEPS app, which could support the implementation of this scalable parenting intervention programme into standard clinical care and, ultimately, improve the outcomes for families of children referred to specialist child and adolescent health services.
Trial registration
: The trial has been prospectively registered on 18 November 2021; ISRCTN 16523503. https://www.isrctn.com/ISRCTN16523503
Modeling Homophily in Dynamic Networks with Application to HIV Molecular Surveillance
Authors: DeGruttola Victor , Nakazawa Masato , Lin Tuo , Liu Jinyuan , Goyal Ravi , Little Susan , Tu Xin , Mehta Sanjay 
Published: October 2022

Abstract
Background: Efforts to control the HIV epidemic can benefit from knowledge of the relationships between the characteristics of people who have transmitted HIV and those who became infected by them. Investigation of this relationship is facilitated by the use of HIV genetic linkage analyses, which allows inference about possible transmission events among people with HIV infection. Two persons with HIV (PWH) are considered linked if the genetic distance between their HIV sequences is less than a given threshold, which implies proximity in a transmission network. The tendency of pairs of nodes (in our case PWH) that share (or differ in) certain attributes to be linked is denoted homophily. Below, we describe a novel approach to modeling homophily with application to analyses of HIV viral genetic sequences from clinical series of participants followed in San Diego. Over the 22-year period of follow-up, clusters of genetically linked viral sequences tended to increase in size. Such increase results from HIV transmissions to new people from those already in the cluster–either directly or through intermediaries.
Methods: Our analytical approach makes use of a logistic model to describe homophily with regard to demographic, clinical, and behavioral characteristics–that is we investigate whether similarities (or differences) between PWH in these characteristics are associated with their sequences being linked. To investigate the performance of our methods, we conducted on a simulation study for which data sets were generated in a way that reproduced the structure of the observed database.
Results: Our results demonstrated strong positive homophily associated with hispanic ethnicity, and strong negative homophily, with birth year difference. The second result implies that the larger the difference between the age of a newly-infected PWH and the average age for an available cluster, the lower the odds of a newly infected person joining that cluster. We did not observe homophily associated with prior diagnosis of sexually transmitted diseases. Our simulation studies demonstrated the validity of our approach for modeling homophily, by showing that the estimates it produced matched the specified values of the statistical network generating model.
Conclusions: Our novel methods provide a simple and flexible statistical network-based approach for modeling the growth of viral (or other microbial) genetic clusters from linkage to new infections based on genetic distance.
A traceable and revocable multi-authority attributed-based access control scheme for mineral industry data secure storage in blockchain
Authors: Zhang Xiaohong , Du Wenqi , Moshayedi Ata Jahangir 
Published: October 2022

Abstract
With the rapid advancements of the mineral industry, the data generated by this industry chain have increased dramatically. To reduce the growing pressure of data storage and security risks, we design a credible on-chain and off-chain collaborative dual storage system that integrates blockchain technology and Interplanetary file system (IPFS), also construct a traceable and revocable multi-authority ciphertext-policy attributed-based encryption (CP-ABE) algorithm to meet the demand of privacy protection and dynamic fine-grained access control. Furthermore, the multi-authority layered authorization with a central authority model distributes system overhead while enabling the platform can be regulated. More importantly, our scheme achieves accurate trace of the malicious users by white-box traceability and capable of implementing indirect immediate user and attribute revocation without requiring key or ciphertext updates. Finally, the proposed scheme is indistinguishability under chosen-plaintext attack (IND-CPA) secure in the standard model. And the performance analysis demonstrates that our scheme is feature-rich, practical and efficient.
Regional Environment and Correlates of Nursing Facilities Closures in Taiwan
Authors: Wang Charlotte , Chang Ching-Yun , Chen Chen-Fen 
Published: October 2022

Abstract
Background: Closing nursing facilities profoundly influences the lives of current residents and access for future residents, yet data are limited on whether regional factors impact the closures of nursing facilities. This study examines the factors associated with closure of nursing facilities in Taiwan, including regional factors (e.g., location of the institution, medical accessibility, bed cost and property price), government regulations (e.g., competent authority, institutional appraisal grade and subsidies) and individual institutional factors (e.g., institutional attributes, managers’ backgrounds).
Methods: We investigated 199 nursing facilities in two neighboring cities with different population densities, property price and medical resources from 2011 to 2019, analyzed their survival probabilities, and explored the risk factors via survival analysis.
Results: For every increase of US$333 in property price per Taiwanese ping [3.305 m2], the hazard ratio was equal to 1.009. Also, institutions in metropolitan areas were less likely to close (hazard ratio, 0.313). Secondly, institutions with lower appraisal levels were more likely to be closed (hazard ratio, 4.874). Finally, operator background, institutional type and bed occupancy were important individual institutional factors related to closure.
Conclusion: Our results suggest that regional factors affect the continued operation of nursing facilities. Institutional operators who rent are easily affected by property price fluctuation, but it is difficult to directly reflect the rent cost on the charges of residents and adjust it according to market fluctuations at any time. Additionally, operating in low-population-density areas proves problematic. Furthermore, people living in non-metropolitan areas use institutional resources accessibility issues need awareness. Our findings show that the government should pay more attention to the risk factors of nursing home closures. We also recommend that the supervising authorities of nursing facilities include these items as part of their regular data collection, examine the relationships between risk factors, observe the impact of regional factors such as population density, the number of institutions, and property prices in the region on institutions, and establish an early warning system.
Maternal and perinatal outcomes associated with COVID-19 among pregnant and postpartum women in public and private hospitals in Brazil
Authors: Arlindo Ellen , Souza Renato Renato T , Costa Maria Laura , Cecatti Jose Jose G , Tedesco Ricardo P , Fernandes Karayna G , Martins-Costa Sérgio HA , Peret Frederico JA , Feitosa Francisco E , Traina Evelyn , Haddad Samira M , Andreucci Carla B Andreucci B , Guida José Paulo , Junior Mário D Correa , Dias Marcos AB , Oliveira Leandro , Junior Elias F Melo , Luz Marília GQ da , Raupp Gustavo dos Santos , Centeno Anne , Filho Edson V Cunha , Vettorazzi Janete 
Published: October 2022

Abstract
Introduction

Brazil is a country of continental dimensions with a great challenge to ascertain adequate healthcare throughout regions. The country registered increased numbers of maternal deaths and adverse perinatal outcomes in pregnant and postpartum women with COVID-19 infection, rising great concern. There is scarce data on findings among public versus private settings.

Objective

to compare maternal and perinatal outcomes in pregnant and postpartum women with SARS-CoV-2 infection, among admissions in public and private maternity hospitals during a period before vaccination.

Study Design

Secondary analysis of the REBRACO initiative, a national multicenter cohort study in Brazil, considering pregnant and postpartum women with suspected or confirmed COVID-19 infections (from February 2020 to February 2021) in 15 maternity centers (2 private and 13 public facilities). Sociodemographic and obstetric characteristics were compared according to the type of hospital care. In addition, clinical, laboratory findings and maternal and perinatal outcomes were compared in both groups. The prevalence ratio (PR) and its 95%CI for each predictor and outcome were calculated.

Results

Of 559 symptomatic cases tested, 289 confirmed COVID-19 cases were included, with 213 (72.7%) women in public hospitals and 76 (27.3%) women in private hospitals. SARS-CoV-2 infection frequency was not significantly different among groups. Women treated at public hospitals had lower schooling (p < 0.001), and half of them declared that their pregnancy was not planned. There were 13 maternal deaths among women treated at public services and no maternal deaths among pregnant women treated at private hospitals (p-value – 0,024). Pregnant women in public hospitals had higher rates of fever (p-value – 0,041), tachypnea (p-value – 0,003), abnormal laboratory findings in liver enzymes (p-value – 0,005), severe acute respiratory syndrome SARS (p-value 0.014) and their neonates presented more neonatal respiratory distress (p-value – 0,020).

Conclusion

Adverse maternal and perinatal outcomes were worse in the public hospital group with increased rates of severe acute respiratory syndrome and neonatal respiratory distress. The alarming difference between the number of deaths in patients treated in the public and private sector calls attention to the need to better understand the differences in access to health and other delays experienced by patients of different social levels.
Bioenergy Potential and Kinetic of Biogas Production in Anaerobic Digestion of Slaughterhouse Effluent
Authors: HOUNDEDJIHOU Dègninou , KODOM Tomkouani , TCHAKALA Ibrahim , BAWA Moctar Limam , DJANEYE Gbandi 
Published: October 2022

Abstract
The authors have requested that this preprint be removed from Research Square.
Ablation of PDIA3 attenuates rheumatoid arthritis by restraining Th1 and Th17 polarization program
Authors: Wang Cong-Yi , Wang Faxi , Yang Chunliang , Luo Jiahui , Rong Shanjie , Zhou Hai-Feng , Lu Wan-Ying , Chen Qi-Jie , Wang Ting , Song Dan-Ni , Liu Jing , Mo Qian , Chen Yu , Liu Yan-Jun , Yan Tong , Gu Wei-Kuan , Zhang Shu , Xiong Fei , Yu Qilin , Zhang Zi-Yun , Eizirik Decio , Dong Lingli , Sun Fei 
Published: October 2022

Abstract
Rheumatoid arthritis (RA) is characterized by the chronic synovial inflammation and bone destruction, which eventually cause joint deformity along with physical disability. Aberrant autoimmune T cell activation underpins RA pathogenesis, but the molecular cues that boost autoimmune T cell program remain elusive. Herein, we report that protein disulfide-isomerase A3 (PDIA3) serves as a critical intrinsic regulator to orchestrate Th1 and Th17 differentiation in RA setting. PDIA3 was remarkably upregulated in CD4 T cells in arthritic mice and positively correlated with key clinic parameters including C-reactive protein (CRP) and Disease Activity Scores 28 (DAS28) in RA patients. Depletion of Pdia3 in CD4 T cells protected mice against RA induction, while CD4 T cells deficient in Pdia3 were featured by the attenuated Th1 and Th17 polarization. Mechanistically, synovial fibroblasts (SFs) derived Wnt5a acts on CD4 T cells to enhance NFAT activity; then, NFAT directly binds to the Pdia3 promoter to facilitate its transcription. Intriguingly, PDIA3 did not exert its function in the endoplasmic reticulum (ER) of CD4 T cells, rather bolstered Th1 and Th17 program mainly through forming a complex with either STAT1 or PKM2 in the nucleus, by which it acted as a transcription coactivator. In accordance, pharmacological inhibition of PDIA3 attenuated collagen-induced arthritis in mice. Collectively, our data unveiled a positive feedback loop in the synovial niche where highly expressed PDIA3 promotes pathogenic CD4 T cell polarization during RA pathogenesis, and targeting PDIA3 to block the crosstalk between SFs and CD4 T cells could be a viable strategy against RA.