bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eosinophilic pneumonia induced by lettuce: A case report
Authors: Nagano Tatsuya , Sekiya Reina , Moriyama Tatsuya , Kawaguchi Aki , Fukui Takafumi , Mimura Chihiro , Kimura Yohei , Ohnishi Hisashi , Kotani Yoshikazu , Nishiura Yoshihiro 
Published: April 2022

Abstract
Background: Lettuce (Lactuca sativa) belongs to the Asteraceae family and is a vegetable widely consumed globally. Although lettuce is extensively cultivated and consumed, lettuce-associated occupational allergy is rarely reported. Herein, we are reporting a case of eosinophilic pneumonia induced by lettuce for the first time.Case presentation: A 56-year-old female lettuce farmer was admitted to the hospital with a low-grade fever, worsening cough, and dyspnoea. A blood test revealed eosinophilia and a high serum IgE concentration. A chest X-ray taken on admission showed an infiltrative shadow in the upper lung field. Chest CT revealed patchy ground glass opacity on the upper lung field and thickening of the bronchial wall. The bronchoalveolar lavage fluid contained 8% eosinophils. The IgG-binding proteins that reacted with the patient’s sera were identified by immunoblot analysis. She was diagnosed as lettuce induced eosinophilic pneumonia and was treated with prednisolone, and her symptoms and radiological findings improved. Wearing a mask and reducing the amount of the crop improved her symptoms the following year.Conclusions: This is the first case report about lettuce-induced eosinophilic pneumonia which occurred in a lettuce farmer. Antigen avoidance has reduced the patient’s symptoms.
Comparison of pedicle screw fixation with or without cement augmentation combined with single-segment isthmic spondylolisthesis in osteoporotic spine
Authors: Tang Yong-chao , Peng Jian-cheng , Guo Hui-zhi , Zhan Chen-guang , Huang Hua-sheng , Ma Yan-huai , Zhang Shun-cong , Xu Yue-rong , Mo Guo-ye 
Published: April 2022

Abstract
Purpose: This study aims to investigate the necessity of cement-augmented pedicle screw fixation in single-segment isthmic spondylolisthesis with osteoporosis.Method: Fifty-nine cases were reviewed retrospectively. Thirty-three cases were in the polymethylmethacrylate-augmented pedicle screw (PMMA-PS) group, and the other 26 cases were in the conventional pedicle screw (CPS) group. Evaluation data included operation time, intraoperative blood loss, hospitalization cost, hospitalization days, rates of fusion, screw loosening, bone cement leakage, visual analog scores (VAS), Oswestry disability index (ODI), lumbar lordosis (LL), pelvic tilt (PT) and sacral slope (SS).Results: The operation time and blood loss in the CPS group decreased significantly compared to those in the PMMA-PS group (P < 0.05). The average hospitalization cost of the PMMA group was significantly higher than that of the CPS group (P < 0.05). There was no significant difference in the average hospital stay between the 2 groups (P > 0.05). The initial and last follow-up postoperative VAS and ODI improved significantly in the two groups (P < 0.05). There were no significant differences in VAS and ODI at each time point between the 2 groups (P > 0.05). The last postoperative spine-pelvic parameters were significantly improved compared with those preoperatively (P < 0.05). In the PMMA-PS group, the fusion rate was 100%. The fusion rate was 96.15% in the CPS group. No significant difference was found between the two groups for the fusion rate (P > 0.05). Nine patients in the PMMA-PS group had bone cement leakage (27.27%). There was no screw loosening in the PMMA-PS group. There were 2 cases of screw loosening in the CPS group. There were no significant differences in screw loosening, postoperative adjacent segment fractures, postoperative infection or postoperative revision between the 2 groups (P > 0.05).Conclusions: The use of PMMA-PS on a regular basis is not recommended for posterior lumbar interbody fusion for the treatment of single-segment isthmic spondylolisthesis with osteoporosis.
Insecticide treated eave nets and window screens for malaria control in Chalinze district, Tanzania: a study protocol for a household randomized control trial
Authors: Odufuwa Olukayode G. , Moore Sarah Jane , Mboma Zawadi Mageni , Muganga Joseph , Bosselmann Rune , Skovmand Ole , Bradley John 
Published: April 2022

Abstract
Background: Long-Lasting Insecticidal nets (LLINs) have contributed to the reduction of malaria in sub-Saharan Africa, including Tanzania. However, they rely on daily user behaviour and high coverage which is difficult to maintain. Also, insecticide resistance among malaria vector mosquitoes is contributing to reduced efficacy of control tools. To overcome these problems, we propose to evaluate a new tool for house modification, the dual active ingredient (dual AI) insecticide treated eave nets (ITENs) in combination with insecticide treated window screens (ITWS) for the control of malaria. Methods: Four hundred and fifty (450) households with intact walls, open eaves without screens or nets on the windows in Chalinze district will be eligible and recruited upon written informed consent. The households will be randomly allocated into two arms: one with ITENs and ITWS installed and the other without. Malaria parasite detection using a quantitative Polymerase Chain Reaction (qPCR) will be conducted shortly after the long rain (June/July, 2022) as the primary outcome and shortly after the short rain (January/February, 2022) as the secondary outcome. Other secondary outcomes include clinical malaria cases, and density of malaria vectors and nuisance after the short rain and long rain. In addition, surveys will be conducted in households with ITENs and ITWS to estimate the intervention’s cost during installation, adverse effects after one month of installation, and presence, fabric integrity and user acceptance after six and twelve months of installation. Bioefficacy and chemical content will be evaluated after 12 months of installation. Discussion: ITENs and ITWS have been shown in Kenya to reduce indoor mosquito density. However, it was not known if the indoor mosquito density reduction translated into reduction of malaria cases. Data from the study will measure the public health value of an additional intervention for malaria control in areas of mosquito insecticide resistance that does not require daily adherence. Trial registration: The study is registered on ClinicalTrials.gov.
A multi-center, prospective, open-label, randomized study to explore efficacy and safety of baricitinib in active primary Sjogren's syndrome patients
Authors: Bai Wei , Yang Fan , Xu Huji , Wei Wei , Li Hongbin , Zhang Liyun , Zhao Yi , Shi Xiaofei , Zhang Yan , Zeng Xiaofeng , Leng Xiaomei 
Published: April 2022

Abstract
BackgroundPrimary Sjogren’s syndrome (pSS) is a systemic autoimmune disease involving multiple organ systems. The Janus kinase/signal transduction and activator of transcription (JAK/STAT) signaling pathway is a key pathway involving the pathogenesis of pSS. Baricitinib, a selective JAK1 and JAK2 inhibitor, has been approved for treatment of active rheumatoid arthritis and reported in treatment of come other autoimmune diseases including systemic lupus erythematosus. We have found that baricitinib might be effective and safety in pSS in a pilot study. However, there is no published clinical evidence of baricitinib in pSS. Hence, we conducted this randomized study to further explore the efficacy and safety of baricitinib in pSS.MethodsThis is a multi-center, prospective, open-label, randomized study to compare the efficacy of baricitinib + hydroxychloroquine (HCQ) with HCQ alone in pSS patients. We plan to involve 87 active pSS patients with European League Against Rheumatism pSS disease activity index (ESSDAI) ≥ 5 from eight different tertiary centers in China. Patients will be randomized (2:1) to receive baricitinib 4mg per day + HCQ 400mg per day or HCQ 400mg per day alone. We will switch HCQ to baricitinib + HCQ if the patient in the latter group has no ESSDAI response at week 12. The final evaluation will be at week 24. The primary endpoint is the percentage of ESSDAI response, or minimal clinically important improvement (MCII), which was defined as an improvement of ESSDAI at least three points at week 12. The secondary endpoints include EULAR pSS patient reported index (ESSPRI) response, change of Physician's Global Assessment (PGA) score, serological activity parameters, salivary gland function test, and focus score on labial salivary gland biopsy.DiscussionThis is the first randomized controlled study to evaluate the clinical efficacy and safety of baricitinib in pSS. We hope that the result of this study can provide more reliable evidence of the efficacy and safety of baricitinib in pSS.Trial registrationClinicalTrials.gov, ID: NCT05016297. Registered 19 Aug 2021.
Development of healthy lifestyle consciousness index for gynecological cancer patients
Authors: Higashiyama Nozomi , Yamaguchi Ken , Yamamoto Yosuke , Ueda Akihiko , Inayama Yoshihide , Egawa Miho , Yamanoi Koji , Taki Mana , Ukita Masayo , Hosoe Yuko , Horie Akihito , Hamanishi Junzo , Mandai Masaki 
Published: April 2022

Abstract
Purpose: Healthy lifestyle is related to quality of life (QOL) after cancer diagnosis and prognosis. However, there are few reports on patients conscious of healthy lifestyle and patients requiring medical providers’ attention regarding healthy lifestyle. We aimed to develop a healthy lifestyle consciousness index (HLCI) for cancer patients and evaluated its validity in gynecological cancer patients.Methods: The HLCI was designed to assess degree of healthy lifestyle consciousness, including items regarding "diet," "exercise," "body weight," and "sleep.” Exploratory factor analysis was performed for dimensionality of the scale; Cronbach’s alpha was calculated to assess internal-consistency reliability. For criterion-based validity, we calculated proportions of stage III/IV gynecological malignancies in those with categorized HLCI scores based on tertiles. Concurrent validity was evaluated between HLCI and other quality of life (QOL) scales including European Organization for Research and Treatment of Cancer QLQ-C30 in limited patients.Results: HLCI comprised five 10-point items (0–45); higher values implied improved healthy lifestyle consciousness. Data from 108 gynecological malignancy patients at Kyoto University Hospital were analyzed. The mean age of subjects was 55.8 years; 36.1% of them had uterine corpus cancer; 34.3% were at stage III/IV of gynecological malignancy. The factor analysis revealed HLCI was unidimensional; the reliability based on Cronbach’s alpha was satisfactory (0.88). The proportions of stage III/IV gynecological malignancies were 25.7%, 33.3%, and 44.4% in those with first (7–24 points), second (25–30 points), and third (31–46 points) tertiles of HLCI score, respectively. For patients with other QOL scales (n=25), the mean scores of global health status of QLQ-C30 were 33.3 50.0, and 83.3 for first, second, and third tertiles of HLCI score, respectively. Conclusion: HLCI was successfully validated; thus, patients with advanced stages or higher QOL might have strong consciousness regarding healthy lifestyle. HLCI may be useful in precision care for improved lifestyles and QOL.
How rainfalls influence urban traffic congestion and its associated economic losses at present and in future: taking cities in the Beijing-Tianjin-Hebei region, China for example?
Authors: Zhou Yi , Mao Sicheng , Zhao Haile , Zhang Guoliang , Chen Xin , Jin Yuling , Xu Lin , Pan Zhihua , An Pingli , Lun Fei 
Published: April 2022

Abstract
Traffic congestion is one of serious problems in cities; rainfalls would exacerbate traffic congestion, and thus result in huge economic losses. However, limited studies focused on how rainfalls influenced traffic congestion and its associated economic losses. Based on detailed hourly data, we estimated how traffic congestion index (TCI) changed with different rainfall intensities in the Beijing-Tianjin-Hebei (BTH) region, China, and we also explored their economic losses. The results illustrated that all cities presented the similar trend of daily traffic congestion, and morning peak occurred 2 hours later on holidays than workdays. Rainfall had significant impacts on traffic congestion for most time windows, except midnight. Traffic congestion increased with rainfall intensities, but smaller cities were more vulnerable to rainfall intensity than megacities. Rainfalls led to 0.98 billion yuan of extra economic losses in 2019, 38% of which occurred under heavy rainfalls. Traffic congestion in 2019 caused a total economic cost of 31.5 billion yuan in the BTH region (0.4% of its GDP), including the recurrent cost and economic losses due to rainfalls; besides, the social cost and direct cost contributed the same share of 49.4%, with 1.2% from the environmental costs. Considering future urban development and climate change, it is beneficial to establish the climate-resilient transportation system for avoiding future serious traffic congestion as well as huge economic losses in future.
Minimally Invasive Oblique Lateral Interbody Fusion for L4-5: Surgical Outcomes and Perioperative Complications
Authors: Cong Hai-Bo , Jin Cheng-Zhen , Cong Zhi-Chao , Yu Zhi-Ping , Kim Jin-Sung 
Published: April 2022

Abstract
Purpose:Oblique lateral interbody fusion is increasingly being used by spine surgeons due to its many advantages. Although clinical analysis of oblique lateral interbody fusion has been assessed in many studies, surgical outcomes and perioperative complications of oblique lateral interbody fusion technique at L4-5 level have been rarely reported. The aim of this study is to investigate surgical outcomes and perioperative complications of oblique lateral interbody fusion for patients with degenerative disorders at L4-5, with it being the most common level of degenerative lumbar spine disease. Methods: Twenty-eight patients who underwent oblique lateral interbody fusion at L4-5 were enrolled. The numeric rating scale and Oswestry disability index score were used to assess clinical and functional outcomes. Radiological data included fusion rate, as well as measured changes in the disc and bilateral foramen height. Perioperative complications were also assessed. Results: The numeric rating scales for back and leg, and Oswestry disability index were significantly improved at postoperative 6-month, 1-year, and 2-year compared with preoperative condition (p < 0.05). The changes in the disc height, bilateral foramen height, and segmental lordotic angle between the preoperative and postoperative periods were significant (p < 0.05). Disc heights at postoperative 6 months, 1 year, and 2 years were 12.1, 12.0, and 11.7mm, respectively. The segmental lordotic angle at postoperative 6 months, 1 year, and 2 years were 14.9, 14.2, and 14.6 degrees, respectively. Fusion rates at postoperative 6-month, 1-year, and 2-year were 85.71%, 96.42%, and 100%, respectively. Complications occurred in 7 of 28 patients, there was no major complication (0%) occurred. Sympathetic chain symptoms were the most common approach-related complications. Conclusion: Oblique lateral interbody fusion could be considered as an acceptable surgical option for degenerative lumbar lesions, especially at L4-5 level.
Assembling plant diversity mitigates greenhouse gas emissions and achieves high nitrogen removal when treating the low-C/N wastewater by constructed wetlands
Authors: Jiang Hang , Du Yuanyuan , Han Wenjuan , Wang Lichunxiao , Xiang Chenxu , Ge Ying , Chang Jie 
Published: April 2022

Abstract
The low carbon-to-nitrogen (C/N) ratio in wastewater will inhibit pollutant removal, and more seriously, it will cause an increment of nitrous oxide (N2O) emissions of constructed wetlands (CWs). Raising the C/N ratio of wastewater is an effective way to solve this problem, while it may cause secondary pollution and is costly. Assembling plant diversity promotes N removal, while the effects of plant diversity and increasing C/N ratio on global warming potential (GWP) combined by N2O and methane (CH4) is lack of comparison. In this study, 108 CW microcosms were established to explore the effects of increasing the C/N ratio from 1 to 5 and assembling plant diversity on N removal and GHG emissions. Results showed that when the C/N ratio was 1, (1) increasing species richness reduced N2O and CH4 emissions then reduced the GWP by 70%; (2) the presence of Arundo donax in microcosms reduced GWP by 72%; (3) an A. donax × Tradescantia fluminensis × Reineckia carnea mixture resulted in a high N removal and decreased the GWP per g N removal by 92% with a cost increment of 0.05 USD per m3 wastewater treated; and (4) as the C/N ratio increasing to 5, the GWP per g N removal of monocultures was reduced by 96%, but the cost increased by at least 0.29 USD per m3 wastewater treated. In summary, configuring plant diversity in CWs is an efficient, clean and cost-effective measure to treat wastewater with a low C/N ratio.
The expression of Lin28 in gastric cancer cells treated with an anticancer bioactive peptide combined with oxaliplatin in vitro and in vivo
Authors: Li Xian , Kang Lihua , Peng Jiaqi , Han Wenyan , Su Xiulan 
Published: April 2022

Abstract
Gastric cancer has become one of the major diseases threatening human health. This study aimed to investigate the effect and mechanism of an anticancer bioactive peptide (ACBP) combined with oxaliplatin (OXA) on MKN-45, SGC7901, and NCI-N87 differentiated human gastric cancer cells and GES-1 immortalized human gastric mucosal epithelial cells. And the therapeutic effect and action mechanism of short-term intermittent ACBP combined with OXA on nude mice with human gastric cancer were investigate. The half-maximal inhibitory concentrations of these agents in these cells were measured by an MTT assay, and cell morphological changes were observed by H&E staining. The expression of Lin28, miR-107, miR-609 and Let-7 in these four cell lines was determined by q-PCR after drug treatment. Lin28 protein expression in these four cell lines treated with these drugs was measured by western blotting. Furthermore, activity and quality of life were observed daily in all tumor-bearing nude mice, and the expression of Lin28 in tumor tissue was determined by immunohistochemistry and RT-PCR. The results showed that ACBP inhibited the proliferation of MKN-45, SGC7901, and NCI-N87 gastric cancer cells in a dose-dependent manner and weakly suppressed the proliferation of GES-1 cells. Moreover, the inhibitory effect on proliferation was stronger in poorly differentiated gastric cancer cells. ACBP, OXA and the combination upregulated Lin28 gene expression in MKN-45 cells and downregulated it in SGC7901 and GES-1 cells. ACBP and the combination therapy downregulated Let-7 expression in MKN-45 cells and upregulated Let-7 expression in SGC7901 cells. The combination of ACBP with OXA has significant anticancer sensitization on oxaliplatin, and also significantly improves the quality of life of tumor-bearing nude mice and reduces the toxic side effects of chemotherapeutic drugs on nude mice. Therefore, ACBP alone and in combination with oxaliplatin influenced the expression of tumor stem cell marker gene Lin28 and regulated the expression of microRNAs specifically regulated by Lin28. In addition, the anticancer effects and attenuated sensitization effects of ACBP may be related to the Lin28/miRNA-107 signaling pathway, acting by inhibiting the proliferation of cancerous stem cells.
The Effects of Daily Autobiographical Memory Training on Memory Bias, Mood and Stress Resilience in Dysphoric Individuals
Authors: Bovy Leonore , Ikani Nessa , Kraats Livia N.M. , Dresler Martin , Tendolkar Indira , Vrijsen Janna N. 
Published: April 2022

Abstract
Negative memory bias refers to the enhanced recall of negative memories and is a prominent cognitive factor causing and maintaining depression. Surprisingly few studies modify this negative recall. The current study used a smartphone-based autobiographical memory training to increase positive memory recall and thereby alter negative memory bias. A total of 96 dysphoric (≥ 13 BDI-II) participants were randomly allocated to a positive, sham or no-training condition, conducted over a period of six days. Positive memory bias (i.e., recalled event evaluation) significantly increased from pre- to post-training after positive and sham intervention, suggesting an unspecific training effect. No transfer to memory specificity, implicit memory bias or depressive symptoms was found, nor was the training effect modulated by pre-existing level of positive memory bias. A post-hoc follow-up measurement during the initial COVID-19 crisis revealed that subjects who benefitted most from either of the trainings maintained their stress levels better during a natural stressful period, compared to those who responded least to the training. Future studies should carefully consider the impact of sham training design. Moreover, it is important to examine transfer effects of bias training as practice in daily life.
The oncogenic roles of GPR176 in ovarian cancer: a molecular target for aggressiveness and gene therapy
Authors: Zheng Hua-chuan , Yang Ning , Xue Hang , Yun Wen-jing , Cui Zheng-guo 
Published: April 2022

Abstract
Introduction:G-protein-coupled receptor 176 (GPR176) is a member of the G-protein coupled receptor (GPCR) 1 family and produces a 515 amino acid glycosylated protein. Materials and Methods: In the present study, GPR176 expression was detected using immunohistochemistry (IHC) and compared with clinicopathological characteristics of ovarian cancer using bioinformatics analysis. GPR176-related genes and pathways were analyzed using bioinformatics analysis. In addition, the effects of GPR176 on the phenotypes of ovarian cancer cells were investigated. Results:GPR176 mRNA expression positively correlated with older age, clinicopathological staging, tumor residual status, and unfavorable survival of ovarian cancer (p < 0.05) but negatively with purity loss, infiltration of B cells, and CD8+ T cells (p < 0.05). Gene Set Enrichment Analysis (GSEA) showed that differential expression of the GPR176 gene was involved in focal adhesion, ECM-receptor interaction, ribosome, oxidative phosphorylation, actin skeleton, cytokine-cytokine receptor interaction, gap junction, and cell adhesion molecules (p < 0.05). STRING and Cytoscape were used to determine the top 10 nodes (FN1, COL1A1, MMP2, COL1A2, COL3A1, THBS1, ACAN, DCN, COL5A1, LUM) which were downregulated in ovarian cancer (p < 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that GPR176-related genes were categorized into the AGE-RAGE signaling pathway in diabetic complication, ECM receptor interaction, protein digestion and absorption, ECM structural constituent and organization, and collagen trimer (p < 0.05). GPR176 overexpression promoted the proliferation, anti-apoptosis, anti-pyroptosis, migration and invasion of ovarian cancer cells with overexpression of N-cadherin, Zeb1, Snail, Twist1, and underexpression of gasdermin D, caspase 1, and E-cadherin. These results indicated that GPR176 might be involved in the progression of ovarian cancer by deteriorating aggressive phenotypes. Conclusion:GPR176 could potentially be used as a biomarker to indicate the aggressive behavior and poor prognosis of ovarian cancer and a target of genetic therapy.