bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome sequencing reveals chromosome fusion and extensive expansion of genes related to secondary metabolism in Artemisia argyi
Authors: Miao Yuhuan , Luo Dandan , Zhao Tingting , Du Hongzhi , Liu Zhenhua , Xu Zhongping , Guo Lanping , Chen Changjie , Peng Sainan , Li Jin Xin , Ma Lin , Ning Guogui , Liu Dahui , Huang Luqi 
Published: June 2022

SummaryArtemisia argyi, as famous as Artemisia annua, is a medicinal plant with huge economic value in the genus of Artemisia and has been widely used in the world for about 3000 years. However, a lack of the reference genome severely hinders the understanding of genetic basis for the active ingredient synthesis of A. argyi. Here, we firstly report a complex chromosome‐level genome assembly of A. argyi with a large size of 8.03 Gb, with features of high heterozygosity (2.36%), high repetitive sequences (73.59%) and a huge number of protein‐coding genes (279 294 in total). The assembly reveals at least three rounds of whole‐genome duplication (WGD) events, including a recent WGD event in the A. argyi genome, and a recent burst of transposable element, which may contribute to its large genome size. The genomic data and karyotype analyses confirmed that A. argyi is an allotetraploid with 34 chromosomes. Intragenome synteny analysis revealed that chromosomes fusion event occurred in the A. argyi genome, which elucidates the changes in basic chromosome numbers in Artemisia genus. Significant expansion of genes related to photosynthesis, DNA replication, stress responses and secondary metabolism were identified in A. argyi, explaining the extensive environmental adaptability and rapid growth characteristics. In addition, we analysed genes involved in the biosynthesis pathways of flavonoids and terpenoids, and found that extensive gene amplification and tandem duplication contributed to the high contents of metabolites in A. argyi. Overall, the reference genome assembly provides scientific support for evolutionary biology, functional genomics and breeding in A. argyi and other Artemisia species.
Control of root‐to‐shoot long‐distance flow by a key ROS‐regulating factor in Arabidopsis
Authors: Jin Tianling , Wu Huiyan , Deng Zhuying , Cai Tingting , Li Junkai , Liu Zhangyong , Waterhouse Peter M. , White Rosemary G. , Liang Dacheng 
Published: June 2022

AbstractInter‐tissue communication is instrumental to coordinating the whole‐body level behaviour for complex multicellular organisms. However, little is known about the regulation of inter‐tissue information exchange. Here we carried out genetic screens for root‐to‐shoot mobile silencing in Arabidopsis plants with a compromised small RNA‐mediated gene silencing movement rate and identified radical‐induced cell death 1 (RCD1) as a critical regulator of root–shoot communication. RCD1 belongs to a family of poly (ADP‐ribose) polymerase proteins, which are highly conserved across land plants. We found that RCD1 coordinates symplastic and apoplastic movement by modulating the sterol level of lipid rafts. The higher superoxide production in rcd1‐knockout plants resulted in lower plasmodesmata (PD) frequency and altered PD structure in the symplasm of the hypocotyl cortex. Furthermore, the mutants showed increased lateral area of tracheary pits, which reduced axial movement. Our study highlights a novel mechanism through which root‐to‐shoot long‐distance signalling can be modulated both symplastically and apoplastically.
Secondary acrodermatitis enteropathica‐like skin findings in a case of methylmalonic acidemia
Authors: Chen Yusha , Li Zhongtao , Liu Chuangwen , Wang Sheng 
Published: June 2022

AbstractMethylmalonic acidemia (MMA) is an autosomal recessive genetic disorder caused by decreased activity of methylmalonyl‐CoA mutase or metabolic disturbance of its coenzyme cobalamin, cutaneous manifestations are rare clinical signs in this disease. Herein, we describe a Chinese boy with MMA fed with a formula lacking branched‐chain amino acids presenting with erythroderma and acrodermatitis enteropathica‐like rash, a homozygous nonsense mutation c.742C>T (p.Gln248*) was identified in the MMAA gene. The pedigree exhibited a non‐Mendelian inheritance pattern which was attributed to maternal uniparental disomy on chromosome 4q26‐q34.1 of the proband, confirmed by chromosomal microarray analysis. Our case highlights the association between skin changes and nutritional deficiency due to therapeutic amino acid restrictions in MMA.
Rapid and cost‐effective detection of Fusarium euwallaceae from woody tissues
Authors: de Jager Mignon M. , Roets Francois 
Published: June 2022

AbstractThe polyphagous shot hole borer (PSHB) Euwallacea fornicatus is an ambrosia beetle that poses a serious threat to hundreds of tree species in invaded countries. In its invaded range, it relies on a mutualistic fungus, Fusarium euwallaceae, for colony establishment and nutrition. Together, the beetle–fungus complex can cause Fusarium dieback disease that can lead to the death of highly susceptible hosts. The key to mitigation of the spread and control of PSHB is its rapid detection. Current identification relies on DNA extraction and sequencing of barcoding genes of the beetle and/or the fungus, which is expensive and time‐consuming. Often the beetle is not present in a sample, and isolation and purification of the fungus is needed before employing molecular procedures. Because PSHB has a strong association with its fungal symbiont, at least in its invaded range, and the fungus can infect trees without successful PSHB colony establishment, a molecular tool that can rapidly detect F. euwallaceae from infected wood would aid monitoring efforts. Here we developed a PCR amplification protocol that targets a species‐specific gene present in F. euwallaceae from within infected wood of various hosts without prior fungal isolation, DNA purification or sequencing. The technique can detect F. euwallaceae within minimal woody material (c.15 mg) and even from degraded samples of many hosts. Additionally, identification of F. euwallaceae using lysate prepared from mycelia provided a reliable secondary measure. This method drastically reduces the time and costs associated with the identification of F. euwallaceae from field samples.
Grasses and field crops reduce the concentration of resting spores of Plasmodiophora brassicae in soil under controlled conditions
Authors: Drury Sarah C. , Sedaghatkish Afsaneh , Gossen Bruce D. , McDonald Mary Ruth 
Published: June 2022

AbstractClubroot, caused by Plasmodiophora brassicae, dramatically reduces yields of brassica crops, and once present in a field, can persist in the soil for many years via long‐lived resting spores. Some nonhost crops can stimulate the germination of resting spores, which reduces clubroot inoculum because the pathogen cannot complete its life cycle in the absence of a living host. The effect of selected grasses and other field crops on the concentrations of spores in soil was assessed in two replicated and repeated short‐duration (8‐week) studies under controlled conditions. The spore concentration declined precipitously in the bare soil control relative to the initial spore concentration over the course of both studies. Cultivars of meadow bromegrass, smooth bromegrass and one cultivar of perennial ryegrass reduced the spore concentration compared to the bare soil control in both repetitions of one study, while two cultivars of perennial ryegrass did not reduce the spore concentration. In a second study, spring wheat, perennial ryegrass, barley and field pea reduced spores compared to the bare soil control, but soybean did not. Our hypothesis that larger root systems would produce larger reductions in spore numbers was not supported; there was no correlation between root weight and reduction in spore concentration. We conclude that perennial grasses, spring cereals and field pea can contribute to the reduction of clubroot spores in soil. The advantage of grass crops over annual crops for clubroot management is that movement of infested soil is minimized over the entire duration of the grass stand.
PpybZIP43 contributes to sucrose synthesis in pear fruits by activating PpySPS3 expression and interacts with PpySTOP1
Authors: Zhang Huping , Tao Xin , Fan Xianwei , Zhang Shaoling , Qin Gaihua 
Published: June 2022

AbstractSucrose is an important factor affecting sweetness and flavor in pear fruits, but the molecular mechanism of sucrose synthesis regulation is relatively unknown. Here, we characterized a transcription factor gene from pear (Pyrus pyrifolia Nakai cv. “Hosui”) fruits, PpybZIP43, and found that the transient overexpression of PpybZIP43 in pear fruits significantly increased the sucrose content and the relative expression level of sucrose phosphate synthase genes (PpySPS3 and PpySPS8). Subcellular localization analysis in tobacco leaves showed that PpybZIP43 was localized in the nucleus. Yeast one‐hybrid, electrophoretic mobility shift assay (EMSA), and dual‐luciferase reporter assays indicated that PpybZIP43 was able to activate the expression of PpySPS3 by binding specifically to the G‐box (CACGTG) element in the promoter. The protein–protein interaction assays using yeast two‐hybrid, bimolecular fluorescence complementation (BiFC), firefly luciferase complementation imaging (LCI), and glutathione S‐transferase (GST) pull‐down demonstrated that PpybZIP43 could directly interact with PpySTOP1 to form a transcription complex. This study is helpful for understanding the molecular basis of sucrose synthesis and accumulation in pear fruits and provides candidate genes for breeding.
Speedometer‐reading performance of senior drivers with cognitive impairment: a comparison of analogue and digital speedometers
Authors: Peng Zhouyuan , Shimosaka Momoyo , Nishimoto Hiroyuki , Kinoshita Ayae 
Published: June 2022

AbstractBackgroundWith the deterioration of cognitive functions, the capability to obtain information with speed, one of the essential elements needed to perform safe driving, may be impacted. We aimed to compare the legibility of analogue and digital speedometers for senior drivers with cognitive impairment, and examined the demographic, cognitive, and driving‐related variables that predict their speedometer‐reading performance.MethodsA total of 50 senior drivers with cognitive impairment were investigated and asked to complete an office‐based speedometer‐reading test using an iPad. Two general types of speedometers (analogue and digital) were tested in this study.ResultsThe age of the participants ranged from 61 to 92 years (mean (SD), 79.10 (6.973)), and 29 were male. The mean (SD) score of the Mini‐Mental State Examination was 22.48 (6.089). The median (QL, QU) scores of the analogue and digital speedometer‐reading tests were 4 (4, 5.25) and 6 (6), respectively. Based on the result of the Wilcoxon signed‐rank test, the score of the digital speedometer‐reading test was significantly higher than that of the analogue one (Z = 4.399, P < 0.001). The results of multiple linear regression analyses show that the scores of the Mini‐Mental State Examination (β = 0.358, P = 0.025), and the trail‐making test‐A (β = −0.443, P = 0.006) predicted the digital speedometer‐reading performance, and they together explain 54.7% of the total variance.ConclusionsA digital speedometer was found to be easier for absolute value reading for senior drivers with cognitive impairment, compared to an analogue speedometer. Senior drivers with subjective cognitive decline may also have impairments in obtaining the speed information through an analogue speedometer. General cognitive function and attention may influence the speed‐reading performance on the digital speedometer.
Examining access to health insurance and health care along the life course to shed light on interactions between farm households’ social needs, social policy and the farm business
Authors: Becot Florence A. , Inwood Shoshanah M. 
Published: June 2022

AbstractRural studies scholars have highlighted the important role farm households play in propping up the farm business along the life course. Yet, little is known about how challenges facing farm households impact the farm business and how social safety nets may interact with household and business development trajectories. We use the example of US farm households’ access to health insurance and health care to develop empirical insights into these research gaps. We draw on a life course conceptual framework integrating the European and US literature and conduct bivariate analysis using age categories as the dependent variable on a primary dataset of 1,114 surveyed farm families. Difficulties meeting health care needs affected respondents at any age. Variations in trade‐offs between consumption, savings and investments as farm households make health‐related decisions hint at compounding effects over the life course on farm structure and transition timing. With biggest differences between households under and over 65 years of age (the threshold for universal public coverage), our findings highlight the importance of considering how social policies shape farm families’ (in) ability to meet their social needs and the connections to farm persistence.
The effects of attitudes on household energy behavior. A study of climate change concern, responsibility, and awareness in European societies
Authors: Verachtert Sari 
Published: June 2022

ABSTRACTObjectiveSeveral attempts in environmental research have been made to investigate which factors are crucial for people to act pro‐environmentally. Yet, research results remain rather inconsistent, especially across countries. The present article aims to explore whether climate change attitudes and household energy use are associated with European citizens, independent of the country.MethodsThis cross‐national study uses items from the recent European Social Survey 2016 “climate change and energy” module measuring people's attitudes to climate change and energy preferences at the European level by using a multilevel analysis.ResultsI found that at the individual level, both climate change concern, responsibility, and awareness are important to explain variance in energy‐saving behavior, although in different ways. The results suggest that using only variables directly related to attitudes is too limited to explain household energy behavior. The present study indicates that gender, income, and education have a significant impact on behavior. At the country level, GDP and unemployment rate seem to predict less in relation to household energy behavior.ConclusionClimate change attitudes seem to have an impact on people's energy‐saving behavior, even after controlling for socioeconomic characteristics. However, it is important to distinguish between different measures of household energy behavior.
Effectiveness of checklists in multidisciplinary rounds for improving patient safety in the pediatric intensive care unit: a systematic review protocol
Authors: dos Santos Alves Daniela Fernanda , da Silva Sarah Oliveira , Ullman Amanda , Maia Flávia de Oliveira Motta 
Published: June 2022

ABSTRACT

Objective:
This review will evaluate the effectiveness of checklist use in multidisciplinary rounds, compared with multidisciplinary rounds without checklists, for improving mortality and patient safety in the pediatric intensive care unit.

Introduction:
Single studies have demonstrated that checklists can potentially improve communication of care between health professionals, reduce adverse events, and increase adherence to best practice guidelines. However, no systematic review of the literature has explored the use of checklists in the pediatric intensive care unit.

Inclusion criteria:
This review will consider studies that include pediatric patients, under 18 years of age, admitted to a pediatric intensive care unit. Identified studies will compare the use of checklists in multidisciplinary rounds with multidisciplinary rounds with no checklists. The studies will also evaluate mortality and patient safety outcomes. We will consider experimental and observational studies, published in any language, with no date restrictions.

Methods:
The search strategy will aim to locate both published and unpublished studies. Databases to be searched include MEDLINE, the Cochrane Library, Web of Science, LILACS, Scopus, Embase, CINAHL, the Center for Reviews and Dissemination, Database of Abstracts of Reviews of Effects, and Epistemonikos. The studies will be screened and those meeting the inclusion criteria will be retained by two independent researchers. Assessment of methodology and data extraction will then be carried out. The data will be presented using a narrative synthesis and the studies will be pooled with a statistical meta-analysis, where possible.

Systematic review registration number:
PROSPERO CRD42021233798.
Experiences of health professionals in screening for postpartum depressive symptoms: a qualitative systematic review protocol
Authors: Brito Ana Paula Almeida , Silva Carla Marins , Riesco Maria Luiza , McArthur Alexa 
Published: June 2022

ABSTRACT

Objective:
This review will assess and synthesize the available qualitative evidence on the experiences of health professionals in screening for postpartum depression.

Introduction:
Postpartum depression is a significant public health problem. Clinical screening is essential to develop appropriate interventions to meet the needs of women and their families. The findings of this review will have important implications for decision-making and policy development for continuous professional development programs that promote evidence-based postpartum depression screening.

Inclusion criteria:
This review will consider studies that explore the experiences of health professionals who screen for postpartum depression in any geographic location at any health care level (primary, secondary, or tertiary). The review will focus on qualitative data, including methods such as phenomenology, grounded theory, ethnography, action research, and feminist research.

Methods:
The review will follow a 3-step search strategy, in line with the JBI methodology for systematic reviews of qualitative evidence. The databases to be searched will include MEDLINE, CINAHL, Embase, Scopus, LILACS, ScienceDirect, PsycINFO, Index Psi Periódicos, and PePsic. Unpublished studies will be searched for in Google Scholar, Cybertesis, Dart-E, EthOS, and OATD. Two independent reviewers will evaluate the included studies for methodological quality and extract data using the JBI data extraction and synthesis tools. There will be no language or date limitations.

Systematic review registration number:
PROSPERO CRD42021253792.
Efficacy of digital nasoalveolar molding in reducing severity of cleft lip or palate malformation in infants: a systematic review protocol
Authors: Chaudhari Prabhat Kumar , Dhingra Kunaal , Dhillon Harnoor , Zere Edlira , Sharan Jitendra , Kumar Amit , Alam Mohammad Khursheed , Marya Anand 
Published: June 2022

ABSTRACT

Objective:
This review will compare the efficacy of nasoalveolar molding plates fabricated using a digital workflow to conventional fabrication methods or no intervention in infants with cleft lip or palate.

Introduction:
Nasoalveolar molding reduces the severity of orofacial defects in infants with cleft lip or palate using a series of adaptable plates for the maxillary arch. The conventional method needs multiple patient visits at short intervals for treatment. A digital workflow can be used to fabricate multiple plates in one appointment, which eliminates human error, reduces the number of appointments, and allocates more time for patient care for the orthodontic team than appliance fabrication.

Inclusion criteria:
This review will consider clinical studies that report the results of digital nasoalveolar molding in infants with cleft lip or palate and compare it to the conventional method or to no treatment. Outcomes of interest will be objective measures of craniofacial form, nasolabial measurements, or palatal form.

Methods:
This review will be conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Databases including PubMed, Embase, Cochrane Oral Health Group Trial Register, and ClinicalTrials.gov will be searched using appropriate keywords. Publications in English will be considered. Screening based on titles and abstracts will be done after de-duplication, followed by full-text reading for selection based on the inclusion criteria. Data extracted from the studies will be tabulated and assessed for risk of bias. If applicable, a meta-analysis of the pooled data will be conducted.

Systematic review registration number:
PROSPERO CRD42020186452
Nursing students’ experiences of a post-licensure practical nurse bridging program: a qualitative systematic review protocol
Authors: McCloskey Rose , Keeping-Burke Lisa , Morris Patricia , Witherspoon Richelle , Knight Holly , Cave Sara 
Published: June 2022

ABSTRACT

Objective:
This systematic review will synthesize the qualitative literature on students’ experiences of a post-licensure practical nurse to registered nurse bridging program.

Introduction:
The worldwide shortage of registered nurses has prompted governments and educational institutions to develop alternative pathways to nursing licensure. One strategy used to increase the supply of registered nurses is bridging programs. These grant practical nurses academic credit for previous educational and practical experience, which allows them to complete a Bachelor of Nursing degree in a shorter period of time. However, attrition in bridging programs is a concern. Understanding the experiences of students enrolled in bridging programs will help identify their specific needs and the educational support needed for them to successfully transition into the registered nursing role.

Inclusion criteria:
This review will include published and unpublished studies that examine the experiences of practical nurses enrolled in bridging programs. Studies published in English will be considered, with no date limitations.

Methods:
Databases to be searched include CINAHL, MEDLINE, Embase, and ERIC. Gray literature will be searched for in ProQuest Dissertations and Theses and GreyNet International. Reference lists of included studies will also be reviewed to identify additional studies. The critical appraisal of selected studies and the extraction of data will be independently undertaken by two reviewers using JBI methodology. The findings will be pooled using meta-aggregation to produce comprehensive synthesized findings. A ConQual Summary of Findings will also be presented.

Systematic review registration number:
PROSPERO CRD42021278408
Print Media Framing of the Olympic Games Before Canadian Referendums: The Cases of Calgary 2026 and Vancouver 2010
Authors: Monaghan Jared F.K. , Rocha Claudio M. 
Published: June 2022

This study used a quantitative content analysis and a qualitative thematic analysis to explore how the Olympic Games were framed in print media prior to two Canadian Winter Olympic referendums. Content-analysis results showed that the salient topics and the tone of newspaper articles were framed more positively prior to the successful Vancouver 2010 referendum compared with the unsuccessful Calgary 2026 referendum. The thematic analysis indicated four themes. First, news discourse emphasized the importance of Olympic vision that is congruent with host city needs. Second, the prominence of health promotion through sport as a reported theme was more associated with a successful bid. Third, the communication and quantification of intangible benefits were reported to be increasingly important so that the value of the Olympics can be assessed fairly against the ever-burgeoning hosting costs. Finally, the Olympic brand has been deteriorating, at least over the last 15–16 years according to print media. Implications for theory and practice are discussed.