bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Family Impact of a Robin Sequence Prenatal Diagnosis
Authors: Ma Christopher Y. , Caprio Ryan M. , Jindal Snigdha , Netson Rebecca , Resnick Cory M. 
Published: October 2022

Objective Assess the impact of prenatal diagnosis of Robin sequence (RS) on parental experience during gestation and early infancy. Design Prospective case-control study. An online survey was administered via email to 44 parents representing 34 unique patients with RS. Participants Parents of children diagnosed with RS and who received mandibular distraction at our tertiary care children's hospital. Participants were separated by the timing of RS diagnosis into prenatal and control postnatal groups. Main Outcome Measures Effects of timing of diagnosis on parents’ preparation, caregiver support, education about the condition, stress, and overall mental health. Results Complete responses were received from 44 parents representing 34 unique patients (50% response rate): prenatal, n = 17; postnatal, n = 27. Prenatal diagnosis improved parents’ satisfaction regarding time to prepare for treatment ( P = .001), stress of uncertainty about their child's health ( P = .018), and stress about the operation(s) their child would need ( P = .001). Both the prenatal (82%) and postnatal (78%) groups reported a negative impact on mental health based on diagnosis timing. All parents in the prenatal group preferred having received a prenatal diagnosis and the majority of the postnatal group (85%) would have preferred to have received the diagnosis prenatally. Conclusions Prenatal diagnosis of RS provided tangible benefits for parents by allowing them to mentally prepare, make plans for delivery and treatment, and become educated about the condition. Parents in both groups reported a negative impact on their mental health based on diagnosis timing and the majority of parents consistently preferred prenatal diagnosis.
Current and Cumulative Stress Experiences: A Model for Arab American Young Adults
Authors: Albdour Maha , Templin Thomas , Zilioli Samuele , Hong Jun Sung , Lumley Mark A. 
Published: October 2022

Background: The effect of multiple stressors on immigrant young adults’ endocrine functioning and health outcomes has not been comprehensively investigated. Aims: This study tested a theoretical model of cumulative and current stressor effects on the hypothalamic–pituitary–adrenocortical (HPA) axis functioning and health-related quality of life (HRQL) among Arab American young adults. Methods: Using a cross-sectional design, we recruited 160 participants, ages 18 to 24 years, from an urban university in the Midwest. Cumulative stress was assessed by self-report measures of childhood adversity, bullying victimization, and perceived ethnic discrimination. Current perceived stress was measured using the Perceived Stress Scale (PSS) while cortisol levels were measured in participants’ hair. Structural equation modeling tested the effects of cumulative and current stress on cortisol and HRQL. Results: Cumulative stress was negatively associated with HRQL (standardized path coefficient = −.51, p < .05). Interestingly, however, cumulative stress was inversely associated with hair cortisol level (standardized path coefficient = −.51, p < .05). Current stress was positively associated with cortisol level (standardized path coefficient = .43, p < .05) and negatively associated with the mental HRQL (standardized path coefficient < −.37, p < .05). Conclusion: Cumulative stress exhibited a different effect on HPA functioning from current perceived stress. Mental health was significantly impaired by both cumulative and current perceived stress. Implications for mental health nursing practice and research among Arab Americans are discussed.
Intraoperative identification of the lateral spinal artery in a case of craniocervical junction dural arteriovenous fistula surgically obliterated
Authors: Ishibashi Ryota , Maki Yoshinori , Ikeda Hiroyuki , Chin Masaki 
Published: October 2022

Background The formation of a dural arteriovenous fistula (DAVF) at the craniocervical junction is rare. Such a fistula can be fed by the branches of the vertebral and external carotid arteries. The lateral spinal artery can branch from the vertebral artery. The feeders of a DAVF at the craniocervical junction are often diagnosed on preoperative angiography, and there is little reported evidence on the intraoperative diagnosis of the lateral spinal artery. Case description An 84-year-old man presented with motor weakness and sensory disturbance of the lower extremities. Edematous changes in the medulla oblongata and cervical spinal cord were observed on magnetic resonance imaging. Cerebral angiography revealed a DAVF fed by a branch of the vertebral artery, with a shunting point located in the dura of the right condyle; the main drain was the anterior spinal vein. The DAVF drain was surgically obliterated to prevent hemorrhagic events and improve neurological symptoms. Intraoperatively, an artery branching from the feeder of the DAVF was identified and preserved. The patient had a good postoperative course, and the neurological symptoms were ameliorated. Follow-up cerebral angiography revealed proximal branching of the lateral spinal artery from the feeding artery of the DAVF. Conclusion A lateral spinal artery was identified intraoperatively while a DAVF at the craniocervical junction was obliterated. This suggests that preoperative imaging should be carefully reviewed, and endovascular procedures should consider such possibilities to avoid adverse ischemic outcomes.
Alcohol consumption and its determinants among pregnant women in Gozamin district, Amhara, Ethiopia, 2020
Authors: Abetew Mulatu Mengaw , Alemu Addisu Alehegn , Zeleke Haymanot , Ayenew Asteray Assmie , Aynalem Fikreselassie Getachew , Kassa Getachew Mullu , Khajehei Marjan 
Published: October 2022

Objective: Alcohol consumption during pregnancy can cause many major severe illnesses to both mothers and their offspring. Despite this, many pregnant women consume both homemade and manufactured alcoholic beverages. We conducted this study to assess the prevalence and determinants of alcohol consumption among pregnant women in Gozamin district, Amhara, Ethiopia, in 2020. Methods: Community-based cross-sectional study was employed from 1 to 30 November, 2020. The participants were recruited using a stratified multi-stage sampling technique. A structured and pretested interviewer-administered questionnaire was utilized for data collection. The collected data were entered into Epi-data Version 3.1 and exported to SPSS Version 25 for further analysis. The model fitness was checked by Hosmer and Lemeshow’s goodness of fit test. Then, logistic regression models were considered to determine the associations of independent variables with the outcome variable. Variables with p < 0.25 in bivariable logistic regression were considered for multivariable logistic regression. Finally, variables with p < 0.05 in multivariable logistic regression were considered as determinants of alcohol consumption. Result: A total of 555 pregnant women participated in this study, making the response rate of 97.4%. The prevalence of alcohol consumption among the participants was 45.6% (95% confidence interval = 41.4–49.2). The determinants of alcohol consumption among the participants were highest wealth index (adjusted odds ratio = 3.21; 95% CI = 1.68–6.14), pre-pregnancy alcohol consumption (adjusted odds ratio = 3.67; 95% confidence interval = 2.36–5.71), poor social support (adjusted odds ratio = 3.08; 95% confidence interval = 1.60–5.94), and unplanned pregnancy (adjusted odds ratio = 1.66; 95% confidence interval = 1.04–2.66). Conclusion: In this study, the prevalence of alcohol consumption was high among the pregnant women. Our findings suggest introduction of policies and interventions that can help reduce alcohol consumption during pregnancy. The health education priority should be creation of awareness about the negative health impacts of alcohol on the health of pregnant mothers and their offspring.
Bridging Gaps in Diabetic Nephropathy Care: A Narrative Review Guided by the Lived Experiences of Patient Partners
Authors: Beaubien-Souligny William , Leclerc Simon , Verdin Nancy , Ramzanali Rizwana , Fox Danielle E. 
Published: October 2022

Purpose of review: Diabetes affects almost a 10th of the Canadian population, and diabetic nephropathy is one of its main complications. It remains a leading cause of kidney failure despite the availability of effective treatments. Sources of information: The sources of information are iterative discussions between health care professionals and patient partners and literature collected through the search of multiple databases. Methods: Major pitfalls related to optimal diabetic nephropathy care were identified through discussions between patient partners and clinician researchers. We identified underlying factors that were common between pitfalls. We then conducted a narrative review of strategies to overcome them, with a focus on Canadian initiatives. Key findings: We identified 5 pitfalls along the diabetic nephropathy trajectory, including a delay in diabetes diagnosis, suboptimal glycemic control, delay in the detection of kidney involvement, suboptimal kidney protection, and deficient management of advanced chronic kidney disease. Several innovative care models and approaches have been proposed to address these pitfalls; however, they are not consistently applied. To improve diabetic nephropathy care in Canada, we recommend focusing initiatives on improving awareness of diabetic nephropathy, improving access to timely evidence-based care, fostering inclusive patient-centered care environment, and generating new evidence that supports complex disease management. It is imperative that patients and their families are included at the center of these initiatives. Limitations: This review was limited to research published in peer-reviewed journals. We did not perform a systematic review of the literature; we included articles that were relevant to the major pitfalls identified by our patient partners. Study quality was also not formally assessed. The combination of these factors limits the scope of our conclusions.
All-Soft Tissue Meniscus Allograft Transplantation: Indications, Techniques, and Results
Authors: Zsidai Bálint , Lucidi Gian Andrea , Winkler Philipp W. , Gnandt Ryan J. , Engler Ian D. , Musahl Volker 
Published: October 2022

Background: Meniscus allograft transplantation (MAT) may be indicated for young patients with joint line pain following subtotal or total meniscectomy. Several different approaches for performing MAT have been described in the literature and are influenced by appropriate patient selection, graft-sizing, and soft-tissue fixation techniques. Clinical studies demonstrate favorable results regarding pain relief and knee function in young patients undergoing MAT, making it a viable option for the treatment of postmeniscectomy syndrome. Indications: Meniscus allograft transplantation is indicated for symptomatic patients following subtotal or total meniscectomy. Selection criteria include patient age below 40 to 45 years, body mass index below 35, chondral changes of grade 2 or less, anatomic or correctable joint alignment, and normal or correctable knee stability. Technique Description: A standard arthroscopy is performed to confirm the indication for MAT, followed by debridement of the meniscus remnant up to the meniscus-capsular junction. The meniscus horns are prepared using a single No. 5 suture, while 4 to 5 No. 2 sutures are passed through the posterior body. The anterior and posterior root tunnels are drilled, and the meniscus is inserted through a posterior vertical arthrotomy using suture passers transmitted via the bone tunnels. The allograft is manipulated into proper position with a probe and the application of axial traction on the posterior root suture. At this point, sutures attached to the posterior horn are passed to the posterior capsule and 6 to 8 inside-out sutures are used to stabilize and fixate the midbody and anterior portion of the allograft. Results: Several clinical studies report good outcomes following MAT with a mean survival rate of approximately 70% at 10-year follow-up and 60% at 15 years. Additionally, some evidence is currently available regarding the long-term chondroprotective effect of MAT. Description/Conclusion: All-soft tissue meniscus allograft transplantation is a feasible approach for substitution of the damaged native meniscus and maintenance of tibiofemoral contact mechanics. Consequently, MAT is an important procedure in the toolkit of knee surgeons providing treatment for young, symptomatic postmeniscectomy patients.
Acquired mutations in BAX confer resistance to BH3-mimetic therapy in acute myeloid leukemia
Authors: Moujalled Donia M. , Brown Fiona C. , Chua Chong Chyn , Dengler Michael A. , Pomilio Giovanna , Anstee Natasha S. , Litalien Veronique , Thompson Ella , Morley Thomas , MacRaild Sarah , Tiong Ing S. , Morris Rhiannon , Dun Karen , Zordan Adrian , Shah Jaynish , Banquet Sebastien , Halilovic Ensar , Morris Erick , Herold Marco J. , Lessene Guillaume , Adams Jerry M. , Huang David C. S. , Roberts Andrew W. , Blombery Piers , Wei Andrew H. 
Published: October 2022

Abstract
Randomized trials in acute myeloid leukemia (AML) have demonstrated improved survival by the BCL-2 inhibitor venetoclax combined with azacitidine in older patients, and clinical trials are actively exploring the role of venetoclax in combination with intensive chemotherapy in fitter patients with AML. As most patients still develop recurrent disease, improved understanding of relapse mechanisms is needed. We find that 17% of patients relapsing after venetoclax-based therapy for AML have acquired inactivating missense or frameshift/nonsense mutations in the apoptosis effector gene BAX. In contrast, such variants were rare after genotoxic chemotherapy. BAX variants arose within either leukemic or preleukemic compartments, with multiple mutations observed in some patients. In vitro, AML cells with mutated BAX were competitively selected during prolonged exposure to BCL-2 antagonists. In model systems, AML cells rendered deficient for BAX, but not its close relative BAK, displayed resistance to BCL-2 targeting, whereas sensitivity to conventional chemotherapy was variable. Acquired mutations in BAX during venetoclax-based therapy represent a novel mechanism of resistance to BH3-mimetics and a potential barrier to the long-term efficacy of drugs targeting BCL-2 in AML.
Effect of antiplatelet agents and tyrosine kinase inhibitors on oxLDL-mediated procoagulant platelet activity
Authors: Zheng Tony J. , Kohs Tia C. L. , Mueller Paul A. , Pang Jiaqing , Reitsma Stéphanie E. , Parra-Izquierdo Iván , Melrose Alexander R. , Yang Liping , Choi Jaewoo , Zientek Keith D. , Sviridov Denis O. , Larson Mark K. , Williams Craig D. , Pamir Nathalie , Shatzel Joseph J. , Reddy Ashok P. , Kievit Paul , Remaley Alan T. , Stevens Jan F. , Hinds Monica T. , McCarty Owen J. T. , Aslan Joseph E. 
Published: October 2022

Abstract
Low-density lipoprotein (LDL) contributes to atherogenesis and cardiovascular disease through interactions with peripheral blood cells, especially platelets. However, mechanisms by which LDL affects platelet activation and atherothrombosis, and how to best therapeutically target and safely prevent such responses remain unclear. Here, we investigate how oxidized low-density lipoprotein (oxLDL) enhances glycoprotein VI (GPVI)-mediated platelet hemostatic and procoagulant responses, and how traditional and emerging antiplatelet therapies affect oxLDL-enhanced platelet procoagulant activity ex vivo. Human platelets were treated with oxLDL and the GPVI-specific agonist, crosslinked collagen-related peptide, and assayed for hemostatic and procoagulant responses in the presence of inhibitors of purinergic receptors (P2YR), cyclooxygenase (COX), and tyrosine kinases. Ex vivo, oxLDL enhanced GPVI-mediated platelet dense granule secretion, α-granule secretion, integrin activation, thromboxane generation and aggregation, as well as procoagulant phosphatidylserine exposure and fibrin generation. Studies of washed human platelets, as well as platelets from mouse and nonhuman primate models of hyperlipidemia, further determined that P2YR antagonists (eg, ticagrelor) and Bruton tyrosine kinase inhibitors (eg, ibrutinib) reduced oxLDL-mediated platelet responses and procoagulant activity, whereas COX inhibitors (eg, aspirin) had no significant effect. Together, our results demonstrate that oxLDL enhances GPVI-mediated platelet procoagulant activity in a manner that may be more effectively reduced by P2YR antagonists and tyrosine kinase inhibitors compared with COX inhibitors.
LARRPM restricts lung adenocarcinoma progression and M2 macrophage polarization through epigenetically regulating LINC00240 and CSF1
Authors: Li Yue , Chen Chen , Liu Hai-lin , Zhang Zhen-fa , Wang Chang-li 
Published: October 2022

Abstract
Background
Long non-coding RNAs (lncRNAs) are critical regulators in lung adenocarcinoma (LUAD). M2-type tumor-associated macrophages (TAMs) also play oncogenic roles in LUAD. However, the involvement of lncRNAs in TAM activation is still largely unknown.

Methods
The expressions of LARRPM, LINC00240 and CSF1 were determined by RT-qPCR. The regulation of LINC00240 and CSF1 by LARRPM was investigated by RNA–protein pull-down, RNA immunoprecipitation, chromatin immunoprecipitation and bisulfite DNA sequencing. In vitro and in vivo gain- and loss-of-function assays were performed to investigate the roles of LARRPM.

Results
The lncRNA LARRPM was expressed at low levels in LUAD tissues and cells. The low expression of LARRPM was correlated with advanced stage and poor survival of patients with LUAD. Functional experiments revealed that LARRPM suppressed LUAD cell proliferation, migration and invasion, and promoted apoptosis. LARRPM also repressed macrophage M2 polarization and infiltration. Taken together, LARRPM significantly restricted LUAD progression in vivo. Mechanistically, LARRPM bound and recruited DNA demethylase TET1 to the promoter of its anti-sense strand gene LINC00240, leading to a decrease in DNA methylation level of the LINC00240 promoter and transcriptional activation of LINC00240. Functional rescue assays suggested that the lncRNA LINC00240 was responsible for the roles of LARRPM in the malignant behavior of LUAD cells. LARRPM decreased the binding of TET1 to the CSF1 promoter, resulting in increased DNA methylation of the CSF1 promoter and transcriptional repression of CSF1, which is responsible for the roles of LARRPM in macrophage M2 polarization and infiltration. The TAMs educated by LUAD cells exerted oncogenic roles, which was negatively regulated by LARRPM expressed in LUAD cells.

Conclusions
LARRPM restricts LUAD progression through repressing both LUAD cell and macrophages. These data shed new insights into the regulation of LUAD progression by lncRNAs and provide data on the potential utility of LARRPM as a target for LUAD treatment.
Comparative analysis of the endophytic bacteria inhabiting the phyllosphere of aquatic fern Azolla species by high-throughput sequencing
Authors: Yang Yan-Qiu , Deng Su-Fang , Yang You-Quan , Ying Zhao-Yang 
Published: October 2022

Abstract
Background
Azolla is a small floating fern living in symbiosis with nitrogen-fixing cyanobacteria and provides a variety of important ecosystem benefits. Previous studies have presented that Azolla harbors diverse bacteria that may play a key role in host fitness and productivity. However, the characteristics of endophytic bacteria inhabiting the phyllosphere of different species of Azolla have not yet been fully understood.

Results
In this study, the 16S ribosomal DNA (rDNA) V5-V7 region of bacteria was determined by Illumina high-throughput sequencing platform to study the diversity and richness of endophytic bacterial communities in the phyllosphere of five Azolla species collected from different countries. A total of 1150 operational taxonomic units (OTUs) were detected for the endophytic bacteria community. According to the α diversity indices, the diversity of bacteria was ordered as Azolla imbricata > A. pinnata > A. filiculoides > A. mexicana > A. caroliniana. The PCoA results displayed that the bacterial communities of A. mexicana and A. caroliniana shared the highest similarity, followed by the similarity between A. pinnata and A. imbricata, and they were significantly distinct from the community of A. filiculoides. The dominant bacteria of Azolla mainly belonged to the phylum of Proteobacteria, followed by Actinobacteria, Chlorobillobacteria, and Firmicutes. In detail, the relative abundance of Proteobacteria in A. imbricata was 52.23%, whereas it was more than 80.00% in the other four species of Azolla. Notably, Herbaspirillum (45.91%, 44.08%) and Methylophilus (29.97%, 37.96%) were the main genera inhabiting A. mexicana and A. caroliniana respectively. Ferrovibrio (18.54%) and Rhizobium (16.68%) were the dominant genera inhabiting A. filiculoides. The group of unidentified genera (41.63%, 44.92%) consisted most of the bacteria in A. imbricata and A. pinnata respectively. Further analysis suggested that the significant different bacteria identified in LDA Effect Size analysis existed Azolla species-specific patterns.

Conclusions
In summary, all results suggested that the diversity and composition of the endophytic bacterial communities were different in Azolla species.
Zinc oxide nanoparticles reduce biofilm formation, synergize antibiotics action and attenuate Staphylococcus aureus virulence in host; an important message to clinicians
Authors: Abdelghafar Aliaa , Yousef Nehal , Askoura Momen 
Published: October 2022

AbstractBackgroundBiofilm-related infections are difficult to be treated because of higher resistance to antimicrobial agents. Current study aims to characterize the influence of zinc oxide nanoparticles (ZnO-NPs) on bothS. aureussusceptibility to antibiotics and pathogenesis.MethodsThe influence of ZnO-NPs on biofilm formation byS. aureuswas characterized by the crystal violet and tube assay. The synergistic effect of ZnO-NPs in combination with antibiotics onS. aureuswas characterized using the checkerboard method. The effect of ZnO-NPs onS. aureuscell surface hydrophobicity and blood hemolysis was investigated. RT-qPCR was used to investigate the effect of ZnO-NPs on the expression of biofilm related genes (icaA, icaRandsarA),katAandsigB. The impact of ZnO-NPs onS. aureuspathogenesis was evaluated using mice infection model.ResultsZnO-NPs exhibited a good antibiofilm activity againstS. aureus. The findings indicate a synergistic antibiofilm effect of combination between ZnO-NPs and tested antibiotics. ZnO-NPs were capable of decreasingS. aureuscell surface hydrophobicity which could account for observed decrease in bacterial biofilm forming capacity. Moreover, ZnO-NPs-treated bacteria exhibited a significant decrease in blood hemolysis relative to control untreatedS. aureus. The expression of biofilm related genes was significantly repressed in ZnO-NPs treated bacteria as compared to untreated cells. Finally, the effect of ZnO-NPs onS. aureuspathogenesis was investigated using mice infection model where ZnO-NPs accelerated healing of wounds in mice as compared to control untreated mice.ConclusionsPresent data support the efficiency of ZnO-NPs as antibiofilm agent in treatment ofS. aureusinfections. This study recommends the incorporation of ZnO-NPs as adjuvant with other antibiotics targetingS. aureusbased on the promising findings obtained herein in order to control infection with this pathogen.