bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions
Authors: De Felice E. , Gonçalves de Andrade E. , Golia M. T. , González Ibáñez F. , Khakpour M. , Di Castro M. A. , Garofalo S. , Di Pietro E. , Benatti C. , Brunello N. , Tascedda F. , Kaminska B. , Limatola C. , Ragozzino D. , Tremblay M. E. , Alboni S. , Maggi L. 
Published: December 2022

AbstractThe hippocampus is a plastic brain area that shows functional segregation along its longitudinal axis, reflected by a higher level of long-term potentiation (LTP) in the CA1 region of the dorsal hippocampus (DH) compared to the ventral hippocampus (VH), but the mechanisms underlying this difference remain elusive. Numerous studies have highlighted the importance of microglia–neuronal communication in modulating synaptic transmission and hippocampal plasticity, although its role in physiological contexts is still largely unknown. We characterized in depth the features of microglia in the two hippocampal poles and investigated their contribution to CA1 plasticity under physiological conditions. We unveiled the influence of microglia in differentially modulating the amplitude of LTP in the DH and VH, showing that minocycline or PLX5622 treatment reduced LTP amplitude in the DH, while increasing it in the VH. This was recapitulated in Cx3cr1 knockout mice, indicating that microglia have a key role in setting the conditions for plasticity processes in a region-specific manner, and that the CX3CL1–CX3CR1 pathway is a key element in determining the basal level of CA1 LTP in the two regions. The observed LTP differences at the two poles were associated with transcriptional changes in the expression of genes encoding forIl-1,Tnf-α,Il-6, andBdnf, essential players of neuronal plasticity. Furthermore, microglia in the CA1 SR region showed an increase in soma and a more extensive arborization, an increased prevalence of immature lysosomes accompanied by an elevation in mRNA expression of phagocytic markersMertkandCd68and a surge in the expression of microglial outward K+currents in the VH compared to DH, suggesting a distinct basal phenotypic state of microglia across the two hippocampal poles. Overall, we characterized the molecular, morphological, ultrastructural, and functional profile of microglia at the two poles, suggesting that modifications in hippocampal subregions related to different microglial statuses can contribute to dissect the phenotypical aspects of many diseases in which microglia are known to be involved.
Competitive ability is a fast-evolving trait between house mouse populations (Mus musculus domesticus)
Authors: Linnenbrink Miriam 
Published: December 2022

Abstract
Background
House mice are commensal animals with a nearly global distribution, structured into well differentiated local populations. Besides genetic differences between the populations, they have also diverged behaviorally over time, whereby it remains open how fast general behavioral characteristics can change. Here we study the competitive potential of two very recently separated populations of the Western house mouse (Mus musculus domesticus) by using two different approaches—one under controlled cage conditions, the other under more natural conditions in enclosures mimicking a secondary encounter condition.

Results
We observe a clear bias in the competitive ability towards one of the populations for both tests. The measured behavioral bias is also reflected in the number of hybrid offspring produced in the enclosures.

Conclusion
Our data suggest that key behavioral characteristics with a direct influence on relative fitness can quickly change during the evolution of populations. It seems possible that the colonization situation in Western Europe, with a rapid spread of the mice after their arrival, would have favored more competitive populations at the expansion front. The study shows the possible impact of behavioral changes on the evolution of populations.
The mechanisms and cross-protection of trained innate immunity
Authors: Hu Shiwei , Xiang Danhong , Zhang Xinlu , Zhang Lan , Wang Shengjie , Jin Keyi , You Liangshun , Huang Jian 
Published: December 2022

AbstractIn recent years, the traditional cognition of immunological memory being specific to adaptive immunity has been challenged. Innate immunity can mount enhanced responsiveness upon secondary stimulation, and a phenomenon is termed trained innate immunity. Trained innate immunity is orchestrated by distinct metabolic and epigenetic reprogramming in both circulating myeloid cells and myeloid progenitor cells in bone marrow, leading to long-term resistance to related and non-related pathogens infections. The induction of trained innate immunity can also polarize innate immune cells towards a hyperresponsive phenotype in the tumor microenvironment to exert antitumor effects. This review will discuss the current understanding of innate immune memory and the mechanisms during the induction of innate immunity, including signaling pathways, metabolic changes, and epigenetic rewriting. We also provide an overview of cross-protection against infectious diseases and cancers based on trained innate immunity.
Discovery of vaccine-like recombinant SARS-CoV-2 circulating in human
Authors: He Daniel Chang , He Cheng-Qiang 
Published: December 2022

AbstractFor viral diseases, vaccination with live attenuated vaccine (LAV) is one of the most effective means for fighting the diseases. However, LAV occasionally overflows from vaccinated individuals circulate in the population with unforeseen consequences. Currently, SARS-CoV-2 LAVs are undergoing clinical trials. In this study, we found that the viruses isolated from Indian SARS CoV-2 infected persons may be candidate LAV-derived strains, indicating the risk of SARS-CoV-2 LAV spillover from vaccinated persons, increasing the complexity of SARS-CoV-2 detection. In addition, the property of frequent recombination of SARS-CoV-2 increases the chance of LAV virulence reversion. Therefore, how to distinguish the LAV viruses from the wild strain and how to avoid the recombination of the circulating vaccine strain and the wild strain are the challenges currently faced by SARS CoV-2 LAV development.
The clinical efficacy of periodontally accelerated osteogenic orthodontics in patients with bone fenestration and dehiscence: a retrospective study
Authors: Chen Ziling , Zhou Huan , Zhang Kaili , Wang Xu , Zhong Liangqiuyue , Hou Yuxia , Chen Yue 
Published: December 2022

Abstract
Purpose
The objective of the study was to explore the effect of periodontally accelerated osteogenic orthodontics (PAOO) in orthodontic patients with bone dehiscence and fenestration in the anterior alveolar region of the mandible.

Methods
A retrospective study was performed in 42 patients with bone dehiscence and fenestrations in the anterior alveolar region of the mandible who underwent the PAOO technique. The bleeding index (BI), probing depth (PD), keratinized gingiva width (KGW), gingival recession level (GRL), and gingival phenotype were recorded and assessed at baseline and 6 and 12 months postoperatively. Cone-beam computerized tomography was used to measure bone volume in terms of root length (RL), horizontal bone thickness at different levels, and vertical bone height at baseline and 6 months and 12 months after surgery.

Results
The sample was composed of 42 patients (22 males and 20 females; mean age, aged 25.6 ± 4.8 years) with 81 teeth showing dehiscence/fenestrations and 36 sites presenting gingival recessions. There was no significant difference in BI, PD, or KGW (between baseline and 6 or 12 months postoperatively) based on the clinical evaluations (P > 0.05). Gingival recession sites demonstrated a significant reduction in the GRL after surgery (P < 0.05). Furthermore, the proportion of teeth with a thick gingival phenotype increased from 33.61% at baseline to 53.13% at the end of the follow-up. In addition, the bone thickness measurements at the mid-root and crestal levels were markedly increased compared with the baseline values (P < 0.05), although the increase in thickness at the apical level was not statistically significant (P > 0.05).

Conclusions
Within the limitations of the study, the results show that the PAOO technique is beneficial to periodontal conditions in terms of soft and hard tissue augmentation. The PAOO procedure may represent a safe and efficient treatment for orthodontic patients with bone dehiscence and fenestration.

Trial registration
This study was approved by the ethics committee of the stomatological hospital affiliated with Xi'an Jiaotong University (xjkqll [2019] No. 016) and registered in the Chinese Clinical Trial Registry (ChiCTR2100053092).
A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage
Authors: Barrit Thibault , Campion Claire , Aligon Sophie , Bourbeillon Julie , Rousseau David , Planchet Elisabeth , Teulat Béatrice 
Published: December 2022

AbstractBackgroundSeedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in plant-pathogen interactions, its role has never been studied during this early phase for the interaction betweenArabidopsis thalianaandAlternaria brassicicola, a seed-transmitted necrotrophic fungus. The aim of the present work was to develop an in vitro monitoring system allowing to study the impact of the fungus onA. thalianaseedling growth, while modulating N nutrition.ResultsThe developed system consists of square plates placed vertically and filled with nutrient agar medium allowing modulation of N conditions. Seeds are inoculated after sowing by depositing a droplet of conidial suspension. A specific semi-automated image analysis pipeline based on the Ilastik software was developed to quantify the impact of the fungus on seedling aerial development, calculating an index accounting for every aspect of fungal impact, namely seedling death, necrosis and developmental delay. The system also permits to monitor root elongation. The interest of the system was then confirmed by characterising how N media composition [0.1 and 5 mM of nitrate (NO3−), 5 mM of ammonium (NH4+)] affects the impact of the fungus on threeA. thalianaecotypes. Seedling development was strongly and negatively affected by the fungus. However, seedlings grown with 5 mM NO3−were less susceptible than those grown with NH4+or 0.1 mM NO3−, which differed from what was observed with adult plants (rosette stage).ConclusionsThe developed monitoring system allows accurate determination of seedling growth characteristics (both on aerial and root parts) and symptoms. Altogether, this system could be used to study the impact of plant nutrition on susceptibility of various genotypes to fungi at the seedling stage.
Usefulness of intraoperative nerve monitoring for giant type AB thymoma combined with an aberrant right subclavian artery: a case report
Authors: Iijima Yoshihito , Ishikawa Masahito , Iwai Shun , Yamagata Aika , Motono Nozomu , Tsuji Hiroyuki , Uramoto Hidetaka 
Published: December 2022

Abstract
Background
Abnormal tumor vascularization and escalating tumor size represent two major impediments that make cancer surgery impossible or complicated.

Case presentation
Herein, we report the case of a giant thymoma (type AB) in a 58-year-old woman who presented with cough and yellow sputum. The thymoma grew extensively from the neck to the upper mediastinum. The patient exhibited an aberrant right subclavian artery and a non-recurrent inferior laryngeal nerve. Intraoperative nerve monitoring facilitated the identification and preservation of vital nerves spanning the neck and chest, including the non-recurrent inferior laryngeal nerve. Furthermore, the tumor was divided naturally along the constriction, and a good field of view was acquired to identify abnormal right subclavian arteries and nerves that ran deep in the tumor and surgical field. The tumor was safely removed without complications using intraoperative nerve monitoring, and the thymoma that grew extensively from the neck to the upper mediastinum and was associated with an aberrant right subclavian artery was resected.

Conclusion
Intraoperative nerve monitoring was helpful in identifying the non-recurrent inferior laryngeal nerve and left recurrent laryngeal nerve.
CRAG: de novo characterization of cell-free DNA fragmentation hotspots in plasma whole-genome sequencing
Authors: Zhou Xionghui , Zheng Haizi , Fu Hailu , Dillehay McKillip Kelsey L. , Pinney Susan M. , Liu Yaping 
Published: December 2022

AbstractThe fine-scale cell-free DNA fragmentation patterns in early-stage cancers are poorly understood. We developed a de novo approach to characterize the cell-free DNA fragmentation hotspots from plasma whole-genome sequencing. Hotspots are enriched in open chromatin regions, and, interestingly, 3′end of transposons. Hotspots showed global hypo-fragmentation in early-stage liver cancers and are associated with genes involved in the initiation of hepatocellular carcinoma and associated with cancer stem cells. The hotspots varied across multiple early-stage cancers and demonstrated high performance for the diagnosis and identification of tissue-of-origin in early-stage cancers. We further validated the performance with a small number of independent case–control-matched early-stage cancer samples.
Topical glucocorticoid application causing iatrogenic Cushing’s syndrome followed by secondary adrenal insufficiency in infants: two case reports
Authors: Matejek Nicola , Hoos Johannes , Holterhus Paul Martin , Bettendorf Markus , Choukair Daniela 
Published: December 2022

Abstract
Background
Iatrogenic Cushing’s syndrome induced by oral and parenteral glucocorticoid administration is a well-known complication. Immediate withdrawal from exogenous steroids can lead to life-threatening adrenal insufficiency. However, Cushing’s syndrome caused by topical treatment with glucocorticoids, such as dexamethasone eye drops or dermal application, is rarely recognized. Young infants in particular are at high risk of suffering from iatrogenic Cushing’s syndrome when treated with highly potent topical glucocorticoids.

Case presentation
We present a 6-month-old Syrian boy with cushingoid face after dermal clobetasol cream treatment and a 2-year-old Iranian girl with severe growth retardation after application of dexamethasone eye drops. Both families have a migration background and language barriers. In both cases no endogenous cortisol secretion was initially detected in serum and in 24-hour collected urine. After dose reduction of glucocorticoids, severity of symptoms was reversible and serum cortisol was detectable.

Discussion and conclusion
Young infants are at high risk of developing Cushing’s syndrome from topically applied highly potent glucocorticoids. Precise recommendations of treatment dosage, duration, and frequency must be given to the parents, and if necessary, with the help of an interpreter. Monitoring of height and weight as well as regular pediatric follow-ups should be scheduled. Physicians should be aware of potential adrenal insufficiency following withdrawal from long-term topical glucocorticoid treatment, and hydrocortisone treatment should be considered.
Optic disc hemorrhage in a young female following mRNA coronavirus disease 2019 vaccination: a case report
Authors: Tsuda Kyohei , Oishi Akio , Kitaoka Takashi 
Published: December 2022

Abstract
Background
Since the development of the coronavirus disease 2019 vaccine, there have been many reports of its adverse effects. While respiratory symptoms are common, many other symptoms in various organs have been reported. Herein, we report a case of optic disc and retinal hemorrhage that developed immediately after coronavirus disease 2019 vaccination.

Case presentation
A healthy 18-year-old Japanese female noticed floater in the left eye 1 day after the second vaccination for coronavirus disease 2019 (Pfizer Inc.). Her visual acuity was 20/20 in the left eye, and Goldmann visual field test showed a relative scotoma around blind spot and in the temporal lower quadrant. It was considered due to subretinal hemorrhage and optic disc swelling. Fundus examination revealed retinal and optic disc hemorrhage. Pupillary reflex was intact and central critical flicker was not impaired, indicating that optic nerve was not involved. There was no sign of inflammation, vascular abnormality, nor history of an intense Valsalva maneuver. The hemorrhage resolved spontaneously within 5 months.

Conclusion
This case expands the clinical presentation of coronavirus disease 2019 vaccination-associated ocular adverse events, and it should be kept in mind when patients with similar symptoms visit clinics. The case report will help clinicians avoid unnecessary and invasive examinations and treatment.
NIDD: an intelligent network intrusion detection model for nursing homes
Authors: Zhou Feng , Du Xin , Li Wenli , Lu Zhihui , Wu Jie 
Published: December 2022

AbstractIn nursing homes using technologies such as IoT, big data, cloud computing, and machine learning, there is a constant risk of attacks such as Brute Force FTP, Brute Force SSH, Web Attack, Infiltration, and Botnet during data communication between individual terminals and the cloud server. Therefore, effectively identifying network communication data is essential to protect data communication security between individual terminals and the cloud server. Aiming at the data mentioned above regarding communication security issues, we propose an intelligent intrusion detection model NIDD (Network Intelligent Data Detection) model that combines deep convolution generation adversarial network (DCGAN) with Light Gradient Boosting Machine (LightGBM) and Shapley Additive exPlanations (SHAP). The NIDD model first generates new attack samples by learning the feature distribution of the existing attack sample data and effectively expands the rare attack samples. Secondly, we use the Light Gradient Boosting Machine (LightGBM) algorithm as the base classifier to train the dataset and start to build the intrusion detection model. Then use Shapley Additive exPlanations (SHAP) to analyze the contribution of the classification results, and adjust the model parameters according to the analysis results. Finally, we obtain the optimal model for the intelligent detection model of network intrusion. This paper conducts experimental tests on the NSL-KDD dataset. The experimental results show that the NIDD model built based on Light Gradient Boosting Machine can detect Brute Force FTP, Brute Force SSH, DoS, Heartbleed, Web Attack, Infiltration, Botnet, PROBE, R2L, and U2R attacks with an accuracy of 99.76%. Finally, we re-verified the NIDD model on the CIC-IDC-2018 dataset. The results once again proved that the NIDD model could solve the data communication security between the nursing robot and the cloud server and the data before the IoT terminal and the cloud server. Communication security provides a sufficient guarantee.
Revisiting the energy-growth-environment nexus in the OECD countries: An application of the CS-ARDL approach
Authors: Vo Duc Hong , Ho Chi Minh , Le Quan Thai Thuong , Vo Anh The 
Published: December 2022

Abstract
Background
This study revisits the energy-growth-environment nexus in the member countries of the Organization for Economic Cooperation and Development (OECD) by examining the role of trade openness, financial development, and urbanization. The cross-sectional augmented distributed lag (CS-ARDL) approach is employed to address the presence of slope homoskedasticity and cross-sectional dependence in the data set.

Results
Our empirical findings fail to confirm the validity of the environmental Kuznets curve (EKC) hypothesis for emissions of carbon dioxide (CO2) during the period researched. CO2 emissions have bidirectional causality with income, the share of renewable energy, and the share of nonrenewable energy. Trade openness, financial development, and urbanization play different roles in the energy-growth-environment nexus. Whereas trade openness increases CO2 emissions, financial development reduces consumption of renewable energy. Urbanization plays a limited role in this nexus.

Conclusions
These findings lead to some policy implications. The close relationship between economic growth, CO2 emissions, and energy consumption is highlighted, which suggests that a policy targeting one component needs to consider the impacts on the other components.
Children education for sustainable control of liver fluke infections
Authors: Qian Men-Bao , Zhou Xiao-Nong 
Published: December 2022

AbstractSignificant disease burden is caused by infections with human liver flukes, including Clonorchis sinensis, Opisthorchis viverrini and O. felineus. Epidemiology is characterized by high burden in the male than the female, and in the elder than the younger. Chemotherapy is the mainstream for morbidity control, but it could not prevent re-infection. Meanwhile, behavioral change on the dietary habit of ingesting raw freshwater fish is challenging. In this opinion, we argue why it is important to educate children for sustainable control of liver fluke infections. Then, the design, development and key messages of an educational cartoon for preventing liver fluke infections is introduced. Technical focuses are emphasized based on a pilot using the education cartoon for preventing clonorchiasis in China. Finally, how to further develop and verify this education strategy is discussed.
Graphical Abstract
First record of the human infection of Brucella melitensis in Kyrgyzstan: evidence from whole-genome sequencing-based analysis
Authors: Kydyshov Kalysbek , Usenbaev Nurbolot , Berdiev Stalbek , Dzhaparova Aigul , Abidova Aziza , Kebekbaeva Nuraiym , Abdyraev Murat , Wareth Gamal , Brangsch Hanka , Melzer Falk , Neubauer Heinrich , Pletz Mathias W. 
Published: December 2022

Abstract
Background
Brucellosis, a zoonosis mainly transmitted by consumption of unpasteurized dairy products as well as direct contact with infected animals, is endemic in Kyrgyzstan. However, Brucella species in humans have not been investigated and the origin of the disease remains poorly known in wide parts of Сentral Asia. Thus, molecular characterization of the circulating strains is a critical first step in understanding Brucella diversity in the country.

Methods
In this study, isolates were collected from patients with suspected brucellosis from different regions in Kyrgyzstan between 2019 and 2020. The detection and identification of Brucella was carried out by Bruce-ladder PCR. Next generation sequencing was used to sequence the 89 Brucella isolates, which were genotyped by cgSNP and cgMLST to identify epidemiological connection between Brucella isolates as well as placing them in the context of the global Brucella phylogeny.

Results
The Brucella strains isolated from all regions of Kyrgyzstan were identified as B. melitensis. Based on cgSNP analysis, 18 sequence types were differentiated. The highest numbers of different sequence types were found in Batken (n = 8), Osh (n = 8) and Jalal-Abad (n = 6) oblasts. According to cgSNP and cgMLST analyses, different B. melitensis lineages circulate in Kyrgyzstan, all of them belonging to the Eastern Mediterranean group of the global Brucella phylogeny with the highest similarity to strains from Turkmenistan, Iran and Turkey.

Conclusion
In the present study, B. melitensis was identified as a causative agent of human brucellosis in Kyrgyzstan and different lineages could be identified. Since this study focused on isolates of human origin, the identity of Brucella species and lineages circulating among animal populations remains elusive. Implementing culture techniques and use of most recent molecular, bioinformatic and epidemiological tools are needed to set up a One Health approach to combat brucellosis in Kyrgyzstan. Further, other Сentral Asian countries need to take part in this effort as brucellosis is a transboundary disease in these regions.

Graphical Abstract