bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds
Authors: Li Mei , Xiao Hong , Su Yongmei , Cheng Danlin , Jia Yan , Li Yingli , Yin Qi , Gao Jieying , Tang Yong , Bai Qunhua 
Published: September 2022

Abstract
The prevention and control of infection by resistant pathogens has become a formidable challenge due to the increasing resistance of pathogens to antibiotics, indicating the urgent need for new effective antimicrobial agents. Probiotic is a potential antibacterial drug that can address pathogenic infections. In this study, the antibacterial formulation consisting of honey and Lactobacillus plantarum (L. plantarum) was investigated the optimal antibacterial formulation, in vitro antibacterial effect and mechanism, as well as their effect on the healing of infected wounds in rat whole skin. The results of in vitro experiments showed that the optimal antibacterial formulation of honey and L. plantarum was: honey 10% + L. plantarum 1×109 CFU/mL. In vitro counts showed that the formulation inhibited more than 80% of Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli) after 24 h of action. In addition, live bacterial counts and growth curves indicate that L. plantarum used honey to promote its own growth and produce acids to inhibit the survival of pathogenic bacteria and biofilm formation(P < 0.001). Furthermore, in vivo results reviewed that the honey- L. plantarum formulation inhibited wound bacterial growth(P < 0.01) and increased wound epithelial granulation tissue formation to accelerate skin wound healing in a rat bacterial infection wound model. Overall, these findings suggest that the honey- L. plantarum formulation provides a new option for the treatment of pathogenic infections and wound healing, which sheds new light on the search for alternatives to antibiotics in the future.
Immunohistochemistry based molecular subtyping of pancreatic neuroendocrine tumor correlates with clinicopathological outcome: A single institute retrospective study
Authors: Mitra Saikat , Sekar Aravind , Khosla Divya , Gupta Vikas , Vaiphei Kim 
Published: September 2022

Abstract
Purpose
Pancreatic neuroendocrine tumors (PanNETs) account for ~3-4% of all pancreatic tumors worldwide. The WHO grading and TNM staging in PanNET do not accurately predict the clinical behaviour and prognosis.
Methods
In this single institute retrospective study, cases of PanNETs were selected. Immunohistochemistry (IHC) for ATRX, DAXX, Menin, ARX and PDX-1 markers were performed on formalin-fixed tissue. FITC-labelled telomere-specific fluorescent in-situ hybridization (FISH) was performed to assess altered telomere lengthening (ALT). The tumors were divided into molecular subgroups based on immunohistochemical expression and the subgroups were correlated with clinical, pathological features and follow-up duration using appropriate statistical methods.
Results
Total 78 cases including 75 PanNET (45 Grade 1, 20 Grade 2 and 10 Grade 3) and 3 pancreatic neuroendocrine carcinoma were identified. ATRX and DAXX mutations were identified in 20.9% and 29.9% of PanNET cases respectively. ATRX mutation was significantly associated with nodal metastasis (p=0.007), higher TNM stage (p=0.004), higher WHO grade (p=0.014), lymphovascular invasion (p <0.001), recurrence (p= 0.025). DAXX mutation was significantly associated with a larger tumor size (p=0.007), higher TNM stage (p=0.011), higher WHO grade (p=0.002), lymphovascular invasion (p=0.001), perineural invasion (p=0.046). Univariate survival analysis revealed significantly lower overall follow-up in ATRX mutant (p<0.001), DAXX mutant (p=0.007) subtypes. ALT positivity correlated with higher TNM stage, lymphovascular invasion and ATRX/DAXX mutation. Multivariate cox-regression analysis showed ATRX mutation as independent predictor of poorer overall survival (HR=10.1, CI=1.3-76.7, p=0.025).
Conclusion
ATRX/DAXX mutant PanNETs have aggressive clinical, histological behaviour and are predictors of poor outcome.
First report of Crassiphiala sp. (Trematoda: Diplostomidae) as an etiological agent of black spot disease in commercial ornamental fish from Brazil
Authors: López-Hernández Danimar , Leibowitz Marcia Pimenta , Pinto Hudson Alves , Leal Carlos Augusto Gomes 
Published: September 2022

Abstract
Ornamental fish are becoming increasingly popular, but the lack of knowledge regarding their various diseases is a major challenge. Skin diseases commonly found in freshwater fish include black spot disease (BSD), which is characterized by melanin deposits around the metacercariae of some trematode species. Since BSD remains poorly understood, this study describes an outbreak of BSD in Etroplus maculatus raised in outdoor ponds at a Brazilian fish farm. Metacercariae samples were collected, examined, and subjected to molecular phylogenetic analysis. The parasites were conspecific to an unnamed species, Crassiphiala lineage 5, recently found in Brazilian birds (Megaceryle torquata). Sequences obtained for longifurcate cercariae of the planorbid snail Biomphalaria straminea from the same region were identical to our metacercariae of Crassiphiala sp. These results suggest that Biompahalaria snails are likely an intermediate host of this parasite on farms where E. maculatus was found to be infected. We provide the first molecular evidence that Crassiphiala are the causative agents of BSD in fish from Brazil. Combatting snails and preventing access of fish-eating birds to outdoor ponds are strategies to control this disease in ornamental fish farms.
An Automatically Verified Prototype of the Android Permissions System
Authors: Cristiá Maximiliano , De Luca Guido , Luna Carlos 
Published: September 2022

Abstract
In a previous work De Luca and Luna presented formal specifications of idealized formulations of the permission model of Android in the Coq proof assistant. This formal development is about 23 KLOC of Coq code, including proofs. This work aims at showing that {log} (‘setlog’)---a satisfiability solver and a constraint logic programming language---can be used as an effective automated prover for the class of proofs that must be discharged in the formal verification of systems such as the one carried out by De Luca and Luna. We show how the Coq model is encoded in {log} and how automated proofs are performed. The resulting {log} model is an automatically verified executable prototype of the Android permissions system. Detailed data on the empirical evaluation resulting after executing all the proofs in {log} is provided. The integration of Coq and {log} as to provide a framework featuring automated proof and prototype generation is discussed.
Elevated Glucose on Admission Was an Independent Risk Factor For 30-Day Major Adverse Cardiovascular Events in Patients with STEMI But Not NSTEMI
Authors: Li Yanan , Wang Qinshi , Pei Junyu , Wang Xiaopu , Zheng Keyang , Zhang Ping , Yan Jiafu 
Published: September 2022

Abstract
Objectives
The purpose of this study was to evaluate the impact of glucose levels on admission on the risk of 30-day major adverse cardiovascular events (MACEs) in patients with acute myocardial infarction (AMI), and to assess the difference in outcome between ST-segment elevation myocardial infarction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) patients.
Methods and Results
This study was a post hoc analysis of the Acute Coronary Syndrome Quality Improvement in Kerala Study, and 13,398 participants were included in the final analysis. There was a positive linear association between glucose levels at admission and the risk of 30-day MACEs in AMI patients [adjusted OR (95%CI): 1.05 (1.03, 1.07), P < 0.001]. Participants were divided according to the glucose quintiles. Compared with participants with admission glucose between 5.4 and 6.3 mmol/L, participants with the highest quintile of glucose level (≥ 10.7 mmol/L) were associated with increased risk of 30-day MACEs in the fully adjusted logistic regression model [adjusted OR (95%CI): 1.82 (1.33, 2.50), P < 0.001]. This trend was more significant in patients with STEMI (P for interaction = 0.036).
Conclusion
In patients with AMI, elevated glucose on admission was associated with the increased risk of 30-day MACEs, but only in patients with STEMI.
Neighbourhood Safety, Fall Indices, Physical Activity Level and Social Participation Restrictions from a Population of Community-Dwelling Older Adults in Nsukka, Enugu State, Nigeria.
Authors: Akosile Christopher Olusegun , Ngwu Nnaemeka Pascal , Okonkwo Uchenna Prosper , Onwuakagba Ifeoma Uchenna , Okoye Emmanuel Chiebuka 
Published: September 2022

Abstract
Background: Restriction in physical activity (PA) and social participation restriction (PR) can be heightened in the presence of fear of fall (FOF), fall experience and perceived unsafe neighbourhood particularly among older adults. Despite the enormous benefits of social participation and physical activity, many older adults still remain vulnerable and this probably account for a significant proportion of health challenges for older adults.
Objective: This study investigated the correlations and relationships among neighborhood safety (NS), physical activity (PA), participation restriction (PR) and fall indices among older adults from selected communities in Nsukka, Enugu state, Nigeria.
Methods: This was a cross-sectional survey of 170 consecutively recruited participants. Socio-demographic variables, co-morbidities and fall prevalence were obtained through interviews. The study instruments include PA neighborhood environment scale – Nigeria (PANES-N), PA scale for elderly (PASE), Participation scale (PS), Modified fall efficacy scale (MFES), and Fall risk assessment tool (FRAT) and fall indices.
Statistical Analysis:Descriptive statistics of mean and standard deviations, frequency counts and percentages, and Inferential statistics of Spearman rank order correlation. The p-value was at 0.05 alpha level.
Results: PR was significantly correlated with NS (r= -0.19, p- 0.01*), fall efficacy (r= 0.13, p- 0.10) and fall risk (r= 0.01, p= 0.91), also existed between fall efficacy and fall risk (r= 0.35, p= <0.001*).
Conclusion: participation restriction has a direct relationship with the neighborhood safety, fall efficacy and fall risk. Also fall efficacy is related to fall risk.
Malaria hotspots and climate change trends in the hyper-endemic malaria settings of Mizoram along the India-Bangladesh borders
Authors: Lalmalsawma Pachuau , Balasubramani K , James Meenu Mariya , Pautu Lalfakzuala , Sarma Devojit Kumar , Nina Praveen Balabaskaran 
Published: September 2022

Abstract
India has made tremendous progress in reducing mortality and morbidity in the last decade. Mizoram State in North-East India is one of the few malaria-endemic regions where malaria transmission has continued to remain high. As Mizoram shares international borders with Bangladesh and Myanmar, malaria control in this region is critical for malaria elimination efforts in all the three countries. For identifying hotspots for targeted intervention, malaria data from 388 public health sub-centers across Mizoram were analyzed in the Geographic Information System. Almost all the sub-centers reporting high Annual Parasite Index (> 10) are located in Mizoram’s districts that border Bangladesh. Getis-Ord Gi* statistic shows most of the sub-centers located along the Bangladesh border in the Lawngtlai and Lunglei districts to be the malaria hotspots. The hotspots also extended into the Mamit and Siaha districts, especially along the borders of Lawngtlai and Lunglei. Analysis of climatic and land use/Land cover datasets obtained from the Global Modelling and Assimilation Office and MODIS-Terra + Aqua satellite shows Mizoram’s western part (Lawngtlai, Lunglei and Mamit districts) to experience similar topographic and climatic conditions as the bordering Rangamati district in the Chittagong division of Bangladesh. Climatic trends in this region from 1981 to 2021, estimated by the Mann-Kendall test and Sen's slope estimates, show an increasing trend in minimum temperature, relative humidity, and rainfall, which could facilitate malaria transmission. A regional coordination and strategic plan is required to eliminate malaria from this hyper-endemic malaria region of North-East India.
Geochemistry and detrital geochronology of sandstones of Barail Group of Indo-Myanmar Range, NE India
Authors: Shijoh Chubala , Pebam James , Vales Savio , Singh L. Somorjit , Singh C. D. , Shijoh Vekhoto 
Published: September 2022

Abstract
The sedimentary sequence of Barail Group preserves the records of depositional history of Indo-Myanmar Range during Eocene-Oligocene. Field investigation, geochemistry and detrital geochronology are used to study its geochemical characteristics, sediment provenance and tectonic setting. Major elements based discrimination plots indicate sediments of this group are derived from quartzose sedimentary sources and deposited in a passive margin tectonic set-up. Barail sandstone display a gentle sloping LREE and more or less flat HREE pattern, and show negative Eu anomaly and positive Sm anomaly. Detrital zircon U-Pb reveal age ranging from 3225 Ma to 90 Ma, with clusters at222–90 Ma, 1215 − 394 Ma, 1849 − 1364 Ma, 2500–2094 Ma and 3225–2094 Ma. These results indicate majority of the sediments of the Barail Group were sourced from the Tethyan Sedimentary Sequence and Trans-Himalayan granitoids and deposited in shallow marine condition to deltaic environment.
Upregulation of GALNT7 in prostate cancer modifies O-glycosylation and promotes tumour growth
Authors: Munkley Jennifer , Scott Emma , Hodgson Kirsty , Calle Beatriz , Turner Helen , Cheung Kathleen , Bermudez Abel , Marques Fernando , Pye Hayley , Yo Edward Christopher , Islam Khirul , Oo Htoo Zarni , McClurg Urszula L. , Wilson Laura , Thomas Huw , Frame Fiona M. , Orozco-Moreno Margarita , Bastian Kayla , Arredondo Hector M , Roustan Chloe , Gray Melissa Anne , Kelly Lois , Tolson Aaron , Mellor Ellie , Hysenaj Gerald , Goode Emily Archer , Garnham Rebecca , Duxfield Adam , Heavey Susan , Stopka-Farooqui Urszula , Haider Aiman , Freeman Alex , Singh Saurabh , Johnston Edward W. , Punwani Shonit , Knight Bridget , McCullagh Paul , McGrath John , Crundwell Malcolm , Harries Lorna , Bogdan Denisa , Westaby Daniel , Fowler Gemma , Flohr Penny , Yuan Wei , Sharp Adam , DeBono Johann , Maitland Norman , Wisnovsky Simon , Bertozzi Carolyn , Heer Rakesh , Guerrero Ramon Hurtado , Daugaard Mads , Leivo Janne , Whitaker Hayley , Pitteri Sharon , Wang Ning , Elliott David , Schumann Benjamin 
Published: September 2022

Abstract
Prostate cancer is the most common cancer in men and it is estimated that over 350,000 men worldwide die of prostate cancer every year. There remains an unmet clinical need to improve how clinically significant prostate cancer is diagnosed and develop new treatments for advanced disease. Aberrant glycosylation is a hallmark of cancer implicated in tumour growth, metastasis, and immune evasion. One of the key drivers of aberrant glycosylation is the dysregulated expression of glycosylation enzymes within the cancer cell. Here, we demonstrate using multiple independent clinical cohorts that the glycosyltransferase enzyme GALNT7 is upregulated in prostate cancer tissue. We show GALNT7 can identify men with prostate cancer, using urine and blood samples, with improved diagnostic accuracy than serum PSA alone. We also show that GALNT7 levels remain high in progression to castrate-resistant disease, and using in vitro and in vivo models, reveal that GALNT7 promotes prostate tumour growth. Mechanistically, GALNT7 can modify O-glycosylation in prostate cancer cells and correlates with cell cycle and immune signalling pathways. Our study provides a new biomarker to aid the diagnosis of clinically significant disease and cements GALNT7-mediated O-glycosylation as an important driver of prostate cancer progression.
Resting-State EEG Reveals Abnormal Microstates Characteristics of Depression with Insomnia
Authors: Cao Qike , Wang Yulin , Ji Yufang , He Zhihui , Lei Xu 
Published: September 2022

Abstract
Background: Previous research has revealed various aspects of resting-state EEG for depression and insomnia. However, the EEG characteristics of depressed patients with co-morbid insomniac are rarely studied, especially EEG microstates that capture the dynamic activities of the large-scale brain network.
Methods:To fill the research gaps, this study collected resting-state EEG data from 32 sub-clinical depressions with co-morbid insomnia (CI), 31 comorbid-free depressions (CFD), and 32 healthy controls (HC). Four topographic maps were generated from clean EEG data after clustering and rearrangement. Temporal characteristics were obtained for statistical analysis, including cross-group variance analysis (ANOVA) and intra-group correlation analysis.
Results: The global clustering of all individuals in the EEG microstate analysis revealed the four previously discovered categories of microstates (A, B, C, and D). The occurrence of microstate B was found to be lower in CI than in CFD. The correlation analysis showed that the total PSQI score was negatively correlated with the occurrence of microstate C in CI (r=-0.354, p<.05). Conversely, there was a positive correlation between SDS scores and the duration of microstate C in CFD (r=0.359, p<.05).
Conclusion: The spatiotemporal dynamics of the brain network can vary due to abnormalities in the visual network corresponding to microstate B in patients with depression and insomnia.. Further investigation is needed for microstate change can be related to high arousal and emotional problems in people suffering from depression and insomnia. Microstates may therefore become crucial neurobiological predictors to forecast the likelihood of future cases of depression and insomnia.
A novel anoikis-related gene signature predicts prognosis in patients with LUAD and reveals immune infiltration
Authors: zhang shaocheng , shao akang , wang wei 
Published: September 2022

Abstract
Background: To generate a signature based on anoikis-related genes (ARGs) and reveals immune infiltration for LUAD patients.
Methods: On the basis of TCGA dataset, we identified specific anoikis-related genes in LUAD. The possible biological pathways of anoikis-related genes were analyzed by KEGG. Lasso and Cox-relative regression methods were used to generate an anoikis-related signature. We constructed a nomogram and GES13213 and GES31210 were used to validate the established risk model. The clinical potency and immune status of ARGs were analyzed by CIBERSORT method and ssGSEA algorithm.
Results: 8 anoikis-related genes (BUB1, CDKN3, IL17A, KIF18A, PCNA, PLK1, UBE2C and TIMP1) were determined to develop a prognostic ARGs. The ARGs could accurately classify LUAD cases with different clinical outcome, and survival analysis revealed that high-risk groups had a lower overall survival rate than low-risk groups. In addition, nomogram also has a high predictive value, studies on immune infiltration and tumor microenvironment indicate that immune cells and their functions may play an important role in tumorigenesis and development. Conclusion: ARGs can be used to stratify the risk and forecast the survival outcome of LUAD patients and provide prominent reference for individualized treatment in LUAD.
Immunogenicity of Adjuvanted versus High-Dose Inactivated Influenza Vaccines in Older Adults: A Randomized Clinical Trial
Authors: Schmader Kenneth E. , Liu Christine K. , Flannery Brendan , Rountree Wes , Auerbach Heidi , Barnett Elizabeth D. , Schlaudecker Elizabeth P. , Todd Christopher A. , Poniewierski Marek , Staat Mary A. , Harrington Theresa , Li Rongxia , Broder Karen R. , Walter Emmanuel B. 
Published: September 2022

Abstract
Background
Adjuvanted inactivated influenza vaccine (aIIV) and high-dose inactivated influenza vaccine (HD-IIV) are U.S.-licensed for adults aged ≥65 years. This study compared serum hemagglutination inhibition (HAI) antibody titers for the A(H3N2) and A(H1N1)pdm09 and B strains after trivalent aIIV3 and trivalent HD-IIV3 in an older adult population.
Results
The immunogenicity population included 342 participants who received aIIV3 and 338 participants who received HD-IIV3. The proportion of participants that seroconverted to A(H3N2) vaccine strains after allV3 (112 participants [32.8%]) was inferior to the proportion of participants that seroconverted after HD-IIV3 (130 participants [38.5%]) at day 29 after vaccination (difference, −5.8%; 95%CI, −12.9% to 1.4%). There were no significant differences between the vaccine groups in percent seroconversion to A(H1N1)pdm09 or B vaccine strains, in percent seropositivity for any of the strains, or in post-vaccination GMT for the A(H1N1)pdm09 strain. The GMTs for the post-vaccination A(H3N2) and B strains were higher after HD-IIV than after aIIV3.
Conclusions
Overall immune responses were similar after aIIV3 and HD-IIV3. For the primary outcome, the aIIV3 seroconversion rate for H3N2 did not meet noninferiority criteria compared with HD-IIV3, but the HD-IIV3 seroconversion rate was not statistically superior to the aIIV3 seroconversion rate.
ClinicalTrials.gov Identifier: NCT03183908
Phytochemical Profile and Biological Activities of Ficus carica Fruit’s Extract
Authors: Saleem Makkia , sharif mian kamran , Butt Masood Sadiq , Faisal Muhammad Naeem , Dijk Gertjan van 
Published: September 2022

Abstract
Ficus carica, (fig) one of the oldest plants cultivated on the earth, is rich in phytochemicals including organic acids, anthocyanins, phenolics, and phytosterols. Thus, the present research was carried out to explore the phytochemical composition of fig fruit extracts (prepared with 100% water and 70% ethanol) including, alkaloids, anthocyanins, flavonoids, and phenolic compounds along with HPLC characterization of flavonoids and phenolics. However, the prepared extract was also analyzed for its antioxidative capacity through ABTS, DPPH, FRAP, and H2O2. Extracts prepared with 70% ethanol have better total alkaloids, flavonoids, and phenolics concentration, whereas 100% aqueous extract was more efficient to extract total anthocyanins. HPLC quantification of flavonoids and phenolics showed fig fruit is a good amount of catechin (ranged from 20.89 to 23.97 mg/100g), flavanone (ranged from 8.79 to 12.49 mg/100g), and gallic acid (ranged from 32.52 to 33.350 mg/100g). Likewise, the extract having higher phytochemical concentration exhibited higher antioxidant activity. Conclusively, the results depict nutritional composition as a function of genetic makeup and ecological conditions. The current study suggests that commercially available fig (especially Afghani) and its ethanolic extract possess substantial phytochemical compounds having antioxidant potential. Thus, fig may act as a functional food or nutraceutical ingredient having the potential to alleviate oxidative stress-mediated diseases.