bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induction of oxidative DNA damage, cell cycle arrest and p53 mediated apoptosis by calcium titanate nanoparticles in MCF-7 breast cancer cells
Authors: Mohamed Hanan R. H. , Ibrahim Maria M. H. , Diab Ayman 
Published: November 2022

AbstractBackgroundThe distinctive properties and high activity of calcium titanate nanoparticles (CaTiO3-NPs) increase their use in many products. However, the cytotoxic and genotoxic effects of CaTiO3-NPs in human cancer cell lines have not been well studied. Therefore, this study was conducted to explore CaTiO3-NPs induced cytotoxicity, genomic instability and apoptosis in human breast cancer (MCF-7) cells.MethodsSulforhodamine B (SRB) and the alkaline comet assays were done to study cell viability and DNA damage induction, respectively. Apoptosis induction and cell cycle distribution were analyzed using flow cytometry. The level of intracellular reactive oxygen species (ROS) was studied, and the expression levels of p53, Bax and Bcl2 genes were also measured.ResultsThe results of the Sulforhodamine B (SRB) cytotoxicity assay showed that viability of MCF-7 cells was not affected by CaTiO3-NPs treatment for 24 h, however, exposure to CaTiO3-NPs for 72 h caused concentrations dependent death of MCF-7 cells. Treatment with CaTiO3-NPs for 72 h caused marked increases in intracellular ROS level and induced DNA damage. Treatment of MCF-7 cells with CaTiO3-NPs also caused MCF-7 cell cycle arrest at the G0 and S phases and s triggered apoptosis of MCF-7 cells by causing simultaneous increases in the expression levels of apoptotic p53 and Bax genes and a decrease in the expression level of anti-apoptotic Bcl2 gene.ConclusionCollectively, it was concluded that CaTiO3-NPs cause time- and concentration-dependent cytotoxic effects in human MCF-7 cells through induction of ROS generation, genomic instability and apoptosis. Thus it is recommended that further in vitro and in vivo studies are therefore recommended to understand the cytotoxic and biological effects of CaTiO3-NPs.
Agreement between 24-hour urine and 24-hour food recall in measuring salt intake in primary school children in Australia
Authors: Santos Joseph Alvin , Bolton Kristy A. , Rosewarne Emalie , Trieu Kathy , Di Tanna Gian Luca , Woodward Mark , Webster Jacqui , Grimes Carley 
Published: November 2022

Abstract
Background
Monitoring salt consumption in children is essential for informing and implementing public health interventions to reduce children’s salt intake. However, collection of 24-hour urines, considered as the most reliable approach, can be especially challenging to school children. This study aimed to assess the agreement between 24-hour urine (24hrU) and 24-hour food recall (24hrFR) in: (1) estimating salt intake in children; (2) classifying salt intakes above the recommended upper level set for children, and; (3) estimating change in mean salt intake over time.

Methods
This study utilised data from two cross-sectional surveys of school children aged 8 to 12 years living in the state of Victoria, Australia. A single 24hrU and 24hrFR were collected from each participant. Suspected inaccurate urine collections and implausible energy intakes were excluded based on pre-defined criteria. The agreement between the two methods was assessed using Bland-Altman methodology, the intraclass correlation coefficient (ICC), and the kappa statistic. The difference between the measured change in salt intake over time using 24hrU and 24hrFR was derived using mixed effects linear regression analysis.

Results
A total of 588 participants provided a 24hrU and 24hrFR. Overall, there was no meaningful difference in mean estimated salt intake between the two methods (− 0.2 g/day, 95% CI − 0.5 to 0.1). The Bland-Altman plot showed wide 95% limits of agreement (− 7.2 to 6.8). The ICC between the two methods was 0.13 (95% CI 0.05 to 0.21). There was poor interrater reliability in terms of classifying salt intake above the recommended upper level for children, with an observed agreement of 63% and kappa statistic of 0.11. The change in mean salt intake over time was 0.2 g/day (− 0.4 to 0.7) based on 24hrU, and 0.5 g/day (− 0.0 to 1.1) based on 24hrFR, with a difference-in-differences of 0.4 g/day (− 0.3 to 1.1).

Conclusions
24hrFR appears to provide a reasonable estimate of mean salt intake as measured by 24hrU in Australian school children. However, similar to previous observations in adults, and of studies exploring other alternative methods for estimating salt intake, 24hrFR is a poor predictor of individual-level salt intake in children.
Financing care for Severe Stigmatizing Skin Diseases (SSSDs) in Liberia: challenges and opportunities
Authors: Smith John Solunta , Diaconu Karin , Witter Sophie , Weiland Stefanie , Zaizay F. Zeela , Theobald Sally , McCollum Rosalind , Kollie Karsor , Kollie Jerry , Berrian Hannah , Hotopf India , Sempe Lucas , Tate Wede , Dean Laura 
Published: November 2022

Abstract
Introduction
Neglected tropical diseases (NTDs) are an important global health challenge, however, little is known about how to effectively finance NTD related services. Integrated management in particular, is put forward as an efficient and effective treatment modality. This is a background study to a broader health economic evaluation, seeking to document the costs of integrated case management of NTDs versus standard care in Liberia. In the current study, we document barriers and facilitators to NTD care from a health financing perspective.

Methods
We carried out key informant interviews with 86 health professionals and 16 national health system policymakers. 46 participants were active in counties implementing integrated case management and 40 participants were active in counties implementing standard care. We also interviewed 16 patients and community members. All interviews were transcribed and analysed using the thematic framework approach.

Findings
We found that decentralization for NTD financing is not yet achieved – financing and reporting for NTDs is still centralized and largely donor-driven as a vertical programme; government involvement in NTD financing is still minimal, focused mainly on staffing, but non-governmental organisations (NGOs) or international agencies are supporting supply and procurement of medications. Donor support and involvement in NTDs are largely coordinated around the integrated case management. Quantification for goods and budget estimations are specific challenges, given the high donor dependence, particularly for NTD related costs and the government’s limited financial role at present. These challenges contribute to stockouts of medications and supplies at clinic level, while delays in payments of salaries from the government compromise staff attendance and retention. For patients, the main challenges are high transportation costs, with inflated charges due to fear and stigma amongst motorbike taxi riders, and out-of-pocket payments for medication during stockouts and food/toiletries (for in-patients).

Conclusion
Our findings contribute to the limited work on financing of SSSD services in West African settings and provide insight on challenges and opportunities for financing and large costs in accessing care by households, which is also being exacerbated by stigma.
A dual deep neural network for auto-delineation in cervical cancer radiotherapy with clinical validation
Authors: Nie Shihong , Wei Yuanfeng , Zhao Fen , Dong Ya , Chen Yan , Li Qiaoqi , Du Wei , Li Xin , Yang Xi , Li Zhiping 
Published: November 2022

Abstract
Background
Artificial intelligence (AI) algorithms are capable of automatically detecting contouring boundaries in medical images. However, the algorithms impact on clinical practice of cervical cancer are unclear. We aimed to develop an AI-assisted system for automatic contouring of the clinical target volume (CTV) and organs-at-risk (OARs) in cervical cancer radiotherapy and conduct clinical-based observations.

Methods
We first retrospectively collected data of 203 patients with cervical cancer from West China Hospital. The proposed method named as SegNet was developed and trained with different data groups. Quantitative metrics and clinical-based grading were used to evaluate differences between several groups of automatic contours. Then, 20 additional cases were conducted to compare the workload and quality of AI-assisted contours with manual delineation from scratch.

Results
For automatic CTVs, the dice similarity coefficient (DSC) values of the SegNet trained with incorporating multi-group data achieved 0.85 ± 0.02, which was statistically better than the DSC values of SegNet independently trained with the SegNet(A) (0.82 ± 0.04), SegNet(B) (0.82 ± 0.03) or SegNet(C) (0.81 ± 0.04). Moreover, the DSC values of the SegNet and UNet, respectively, 0.85 and 0.82 for the CTV (P < 0.001), 0.93 and 0.92 for the bladder (P = 0.44), 0.84 and 0.81 for the rectum (P = 0.02), 0.89 and 0.84 for the bowel bag (P < 0.001), 0.93 and 0.92 for the right femoral head (P = 0.17), and 0.92 and 0.91 for the left femoral head (P = 0.25). The clinical-based grading also showed that SegNet trained with multi-group data obtained better performance of 352/360 relative to it trained with the SegNet(A) (334/360), SegNet(B) (333/360) or SegNet(C) (320/360). The manual revision time for automatic CTVs (OARs not yet include) was 9.54 ± 2.42 min relative to fully manual delineation with 30.95 ± 15.24 min.

Conclusion
The proposed SegNet can improve the performance at automatic delineation for cervical cancer radiotherapy by incorporating multi-group data. It is clinically applicable that the AI-assisted system can shorten manual delineation time at no expense of quality.
On the existence and approximation of solutions of generalized equilibrium problem on Hadamard manifolds
Authors: Oyewole O. K. , Jolaoso L. O. , Aremu K. O. , Aphane M. 
Published: November 2022

AbstractIn this paper, we study the existence of solution of the generalized equilibrium problem (GEP) in the framework of an Hadamard manifold. Using the KKM lemma, we prove the existence of solution of the GEP and give the properties of the resolvent function associated with the problem under consideration. Furthermore, we introduce an iterative algorithm for approximating a common solution of the GEP and a fixed point problem. Using the proposed method, we obtain and prove a strong convergence theorem for approximating a solution of the GEP, which is also a fixed point of a nonexpansive mapping under some mild conditions. We give an application of our convergence result to a solution of the convex minimization problem. To illustrate the convergence of the method, we report some numerical experiments. The result in this paper extends the study of the GEP from the linear settings to the Hadamard manifolds.
Removal of phosphates using eggshells and calcined eggshells in high phosphate solutions
Authors: Park Jong-Hwan , Choi Ah-Young , Lee Su-Lim , Lee Jae-Hoon , Rho Jun-Suk , Kim Seong-Heon , Seo Dong-Cheol 
Published: November 2022

AbstractThis study was conducted to evaluate the phosphate sorption properties of eggshell (ES) and calcined ESs (C-ESs) in a high-concentration phosphate solution. The C-ESs yield decreased rapidly at 900 °C, indicating that the CaCO3 constituting the ES was converted to CaO by the high calcination temperature. The optimum calcination temperature for phosphate removal using C-ES was 900 °C. The actual sorption amount of phosphate by ES and C-ES900 was in agreement with the Langmuir isothermal sorption equation, and the maximum sorption capacities derived from this equation were 178.6 and 270.3 mg/g, respectively. The sorption rate of phosphate by ES and C-ES900 was divided into two stages: an initial fast sorption stage, followed by a slow sorption stage. The sorption of phosphate by ES was dominantly influenced by the initial pH and salt concentration, whereas C-ES900 exhibited a constant sorption capacity regardless of environmental changes. The SEM–EDS and XRD results demonstrated that phosphate was successfully adsorbed on the ES and C-SE900 surfaces. In this study, it was found that the sorption of phosphate by ES occurred via ion exchange and precipitation reactions and that the sorption of phosphate by C-ES900 was dominantly affected by precipitation. Above all, C-ES can be applied as an effective adsorbent for removing high concentrations of phosphate under a wide range of environmental conditions.
Case report: challenges in monitoring and treatment of anthracycline induced cardiotoxicity in young adults with osteosarcoma
Authors: Heemelaar Julius C. , Janson Jeroen , Braun Jerry , Speetjens Frank M. , van de Sande Michiel A. J. , Hugo Juan D. V. , Barge-Schaapveld Daniela Q. C. M. , Beeres Saskia L. M. A. , Tops Laurens F. , Gelderblom Hans , Antoni M. Louisa 
Published: November 2022

AbstractNeo(adjuvant) systemic treatment regimens containing anthracyclines such as doxorubicin cause a significant risk of heart failure. These regimens are one of the corner stones of osteosarcoma treatment, and therefore several guidelines are in place to steer cardiotoxicity monitoring through baseline risk stratification and cardiac surveillance during and after completion of cancer therapy.Importantly, baseline risk stratification modules are dependent on age, prior cardiovascular disease and cardiovascular risk factors. Because the majority of osteosarcoma patients are below 30 years of age these criteria rarely apply and most patients are assigned to low or medium risk categories, whereas cardiovascular complications have profound impact on morbidity and mortality in this young population. Therefore, cardiac surveillance is very important in this group for timely detection of cardiotoxicity. Moreover, when severe cardiotoxicity that requires advanced heart failure treatment occurs, a cancer diagnosis has significant implications on treatment options, i.e. mechanical circulatory support and heart transplantation.These challenges are presented in this case of a patient without clinical risk factors admitted with cardiogenic shock requiring advanced heart failure treatment within 1 month after completion of doxorubicin containing chemotherapy for the treatment of high grade osteosarcoma.
Cerebellar irradiation does not cause hyperactivity, fear, and anxiety-related disorders in the juvenile rat brain
Authors: Wang Yafeng , Xie Cuicui , Xu Yiran , Zhang Yaodong , Zhu Changlian , Zhou Kai 
Published: November 2022

Abstract
Background
The cerebellum is involved in hyperactivity, fear, and anxiety disorders that could be induced by whole-brain irradiation (WBI). However, whether cerebellar irradiation alone (CIA) could induce these disorders is unknown. We investigated the effect of CIA in an animal model.

Methods
Eleven-day-old rat pups underwent a single 3-Gy dose of either WBI (n = 28) or CIA (n = 20), while 34 rat pups were sham-irradiated (controls). Cell death was evaluated in the subgranular zone of the hippocampus by counting pyknotic cells after haematoxylin/eosin staining at 6 h after irradiation for 10, 8, and 9 pups, respectively. Behavioural changes were evaluated via open-field test at 6 weeks for 18, 12, and 25 pups, respectively. Unpaired two-tailed t-test and one-way and two-way repeated ANOVA were used.

Results
Massive cell death in cerebellar external granular layer was detected at 6 h after CIA (1,419 ± 211 mm, mean ± S.E.M. versus controls (68 ± 12 mm) (p < 0.001)), while no significant difference between CIA (1,419 ± 211 mm) versus WBI (1,433 ± 107 mm) (p = 0.955) was found. At open-field behavioural test, running distance, activity, wall distance, middle zone visit times, and duration were higher for WBI versus controls (p < 0.010), but no difference between CIA and controls was found (p > 0.05).

Conclusions
Although the cerebellum is involved in hyperactivity, fear, and anxiety disorders, CIA did not induce these disorders, indicating that WBI-induced cerebellar injury does not directly cause these behavioural abnormalities after WBI. Thus, targeting the cerebellum alone may not be enough to rescue or reduce these behavioural abnormalities after WBI.
Using the derived 28-joint disease activity score patient-reported components (DAS28-P) index as a discriminatory measure of response to disease-modifying anti-rheumatic drug therapy in early rheumatoid arthritis
Authors: Pisaniello Huai Leng , Whittle Samuel L. , Lester Susan , Menz Fiona , Metcalf Robert , McWilliams Leah , Hill Catherine L. , Proudman Susanna 
Published: November 2022

Abstract
Background
The 28-joint disease activity score (DAS28) is a widely used measure to assess disease activity in rheumatoid arthritis (RA). The DAS28-P index, a derived proportion of the patient-reported components (joint tenderness and patient global assessment) within the DAS28, has been utilized as a discriminatory measure of non-inflammatory pain mechanisms in RA. This study aimed to evaluate the use of the DAS28-P index as a predictor of treatment response in early RA.

Methods
Patients with early RA enrolled in a supplemental fish oil clinical trial received a combination of disease-modifying anti-rheumatic drugs (DMARDs) according to a ‘treat-to-target’ protocol. First, consecutive measures of the DAS28-P index, derived from the DAS28-erythrocyte sedimentation rate (DAS28-ESR), at each visit over a 1-year period were estimated for each patient. Then, distinct subgroups of treatment responders based on the trajectories of the DAS28-P indices were identified using bivariate k-means cluster analysis. Data on baseline predictors as well as longitudinal outcomes of disease impact and DMARD use over a 1-year period and radiographic progression over a 3-year period were collected and analyzed using a random intercept, population-averaged generalized estimating equation model.

Results
121 patients were included (74% female; mean age of 57; median of 16 weeks of active disease) and a 3-cluster model was identified—the ‘Responders’ group (n = 58; 48%), the ‘Partial Responders’ group (n = 32; 26%), and the ‘Non-Responders’ group (n = 31; 26%). The ‘Partial Responders’ group had consistently higher proportions of the DAS28-P index throughout the study period and had minimal radiographic progression over time, with the lowest joint erosion score of 0.9 [95% confidence interval (CI) 0.2, 1.6], observed at the 3-year follow-up. At 52 weeks, the methotrexate dose was higher for both ‘Partial Responders’ and ‘Non-Responders’ groups (18.5 mg [95% CI 15.5, 21.5] and 18.6 mg [95% CI 15.3, 21.8] respectively), when compared with the ‘Responders’ group (12.8 mg [95% CI 14.7, 20.9]).

Conclusions
Persistently high DAS28-P index scores are useful to distinguish poor patient global assessment and excessive treatment escalation in early RA, suggestive of underlying non-inflammatory pain contributing to higher disease activity score. Early identification of patients with discordant subjective and objective components of composite disease activity measures may allow better tailoring of treatment in RA.
Dynamic Risk Prediction of 30-Day Mortality in Patients With Advanced Lung Cancer: Comparing Five Machine Learning Approaches
Authors: Vesteghem Charles , Szejniuk Weronika M. , Brøndum Rasmus F. , Falkmer Ursula G. , Azencott Chloé-Agathe , Bøgsted Martin 
Published: November 2022

PURPOSE Administering systemic anticancer treatment (SACT) to patients near death can negatively affect their health-related quality of life. Late SACT administrations should be avoided in these cases. Machine learning techniques could be used to build decision support tools leveraging registry data for clinicians to limit late SACT administration. MATERIALS AND METHODS Patients with advanced lung cancer who were treated at the Department of Oncology, Aalborg University Hospital and died between 2010 and 2019 were included (N = 2,368). Diagnoses, treatments, biochemical data, and histopathologic results were used to train predictive models of 30-day mortality using logistic regression with elastic net penalty, random forest, gradient tree boosting, multilayer perceptron, and long short-term memory network. The importance of the variables and the clinical utility of the models were evaluated. RESULTS The random forest and gradient tree boosting models outperformed other models, whereas the artificial neural network–based models underperformed. Adding summary variables had a modest effect on performance with an increase in average precision from 0.500 to 0.505 and from 0.498 to 0.509 for the gradient tree boosting and random forest models, respectively. Biochemical results alone contained most of the information with a limited degradation of the performances when fitting models with only these variables. The utility analysis showed that by applying a simple threshold to the predicted risk of 30-day mortality, 40% of late SACT administrations could have been prevented at the cost of 2% of patients stopping their treatment 90 days before death. CONCLUSION This study demonstrates the potential of a decision support tool to limit late SACT administration in patients with cancer. Further work is warranted to refine the model, build an easy-to-use prototype, and conduct a prospective validation study.
On the influence of Maxwell-Chern-Simons electrodynamics in nuclear fusion involving electronic and muonic molecules
Authors: Caruso Francisco , Oguri Vitor , Silveira Felipe , Troper Amos 
Published: November 2022

Abstract
New results recently obtained (Caruso F. et al., Ann. Phys. (N.Y.), 443 (2022) 168943). established some non-relativistic ground state solutions for three-body molecules interacting through a Chern-Simons model. Within this model, it was argued that the Chern-Simons potential should not help improve the fusion rates by replacing electrons with muons, in the case of particular muonic molecules. This achievement motivated us to investigate quantitatively whether or not the Maxwell-Chern-Simons electrodynamics could influence positively, for example, the probability of having a muon-catalyzed fusion; its contribution to electronic molecules is also considered in this letter. The principal factors related to the probability of elementary nuclear fusion are therefore numerically calculated and compared with their analogs admitting other forms of interaction like −1/ρ and ln(ρ). The analysis carried on here confirms that one should not expect a significant improvement in nuclear fusion rates in the case of muonic molecules, although, surprisingly, the same is not true for electronic molecules, compared with other theoretical predictions. Numerical predictions for the fusion rates for ppe, pp
μ, dde and dd
μ molecules are given as well as the predicted value for the tunneling rate for these molecules.