bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rancang Bangun Sistem Informasi Akuntansi Di Stiki Copy Center
Authors: Haris Lucky Hardiyanti Kartika , Ardiana Dewa Putu Yudhi , Udayana I Putu Agus Eka Darma 
Published: January 2022

Abstract STIKI Copy Center is one place of business that provides print, copy, scan and sells office stationery services. Financial reporting process that occurs in STIKI Copy Center is still not in accordance with the financial statements should be and still use Ms.Excel which makes the owner feel difficulty in the process of calculating the current formula. The financial statements made at Ms.Excel can be manipulated by someone or the owner can do inputting with the same data so that it can cause redundancy and can also cause incompatibility of data and data that could be lost or deleted if infected by a virus or accidentally deleted.
This research aims to design and build an accounting information system at STIKI Copy Center which begins with the stages of identifying problems, conducting data collection, analyzing system modeling using UML, building systems using the laravel framework and MySQL database. The results of this research are financial applications based website that will process the transaction income and expenses and print reports general ledger, balance sheet, income statement, statement of changes in capital and is also equipped with a revenue graph for each month. From the test results by using black box testing can be seen that the system has been going well as expected
Роль міжнародної допомоги у відновленні роботи переміщених закладів вищої освіти: досвід СНУ ім. В. Даля
Authors: Поркуян О.В. , Семененко І.М. 
Published: January 2022

Освіта є одним з основних прав людини, що закладає ба-зис для розвитку країни в цілому та її окремих регіонів. В Україні функціонують значна кількість закладів вищої освіти, які сприяють реалізації цього права. В Україні іс-нують переміщені заклади вищої освіти, які вимушено змінили місце свого розташування у 2014 році внаслідок збройного конфлікту. Вимушене переміщення закладів освіти суттєво вплинуло на сталість їх функціонування через втрату ними матеріально-технічної бази, кадрово-го потенціалу, вплив негативних чинників, як от близь-кість до лінії зіткнення, від’їзд населення з Донецької та Луганської областей тощо. Стаття спрямована на дос-лідження ролі міжнародної допомоги у відновленні робо-ти переміщених закладів вищої освіти, зокрема, на прик-ладі Східноукраїнського національного університету імені Володимира Даля (СНУ ім. В. Даля).
В статті зазначено, що переміщення закладів вищої освіти на нове місце було складним процесом, який міг су-проводжуватися конфліктами, утрудненою адаптацією тощо. Створена у 2016 році Рада ректорів переміщених закладів вищої освіти, як консультативно-дорадчий орган при Міністерстві освіти і науки України, сприяла підт-римці цих закладів та захисту прав учасників освітнього процесу. Проте найсуттєвішу підтримку відновленню функціонування надала міжнародна спільнота через різ-номанітні проєкти і програми допомоги.
Сьогодні СНУ ім. В. Даля, як і решта переміщених закла-дів вищої освіти, став повноцінним учасником регіональ-ного розвитку, осередком згуртованості і активного гро-мадянства, майданчиком для діалогу між різними регіо-нальними стейкхолдерами. Завдяки отриманню міжнаро-дної допомоги в університеті було створено різні майдан-чики і центри, діяльність яких сприяє розвитку м’яких на-вичок здобувачів, партнерству і співпраці з громадами, сталому розвитку регіону в цілому. При СНУ ім. В. Даля створено дві громадські організації, з якими за підтримки міжнародних організацій реалізовано суттєву кількість проєктів. В статті наведені приклади різних проєктів, що реалізовано СНУ ім. В. Даля, зокрема, відзначено про важливість поточного проєкту ЄС «Відродження пере-міщених університетів: посилення конкурентоспромож-ності, підтримка громад» (REDU), який спрямований на досягнення багатьох різнопланових цілей. На основі пред-ставлених проєктів, в статті узагальнено основні ре-зультати діяльності закладів вищої освіти, досягненню яких сприяє міжнародна підтримка.
Sources of CO2 Produced in Freshly Thawed Pleistocene-Age Yedoma Permafrost
Authors: Melchert Jan Olaf , Wischhöfer Philipp , Knoblauch Christian , Eckhardt Tim , Liebner Susanne , Rethemeyer Janet 
Published: January 2022

The release of greenhouse gases from the large organic carbon stock in permafrost deposits in the circumarctic regions may accelerate global warming upon thaw. The extent of this positive climate feedback is thought to be largely controlled by the microbial degradability of the organic matter preserved in these sediments. In addition, weathering and oxidation processes may release inorganic carbon preserved in permafrost sediments as CO2, which is generally not accounted for. We used 13C and 14C analysis and isotopic mass balances to differentiate and quantify organic and inorganic carbon released as CO2 in the field from an active retrogressive thaw slump of Pleistocene-age Yedoma and during a 1.5-years incubation experiment. The results reveal that the dominant source of the CO2 released from freshly thawed Yedoma exposed as thaw mound is Pleistocene-age organic matter (48–80%) and to a lesser extent modern organic substrate (3–34%). A significant portion of the CO2 originated from inorganic carbon in the Yedoma (17–26%). The mixing of young, active layer material with Yedoma at a site on the slump floor led to the preferential mineralization of this young organic carbon source. Admixtures of younger organic substrates in the Yedoma thaw mound were small and thus rapidly consumed as shown by lower contributions to the CO2 produced during few weeks of aerobic incubation at 4°C corresponding to approximately one thaw season. Future CO2 fluxes from the freshly thawed Yedoma will contain higher proportions of ancient inorganic (22%) and organic carbon (61–78%) as suggested by the results at the end, after 1.5 years of incubation. The increasing contribution of inorganic carbon during the incubation is favored by the accumulation of organic acids from microbial organic matter degradation resulting in lower pH values and, in consequence, in inorganic carbon dissolution. Because part of the inorganic carbon pool is assumed to be of pedogenic origin, these emissions would ultimately not alter carbon budgets. The results of this study highlight the preferential degradation of younger organic substrates in freshly thawed Yedoma, if available, and a substantial release of CO2 from inorganic sources.
The Association Between the Decline of eGFR and a Reduction of Hemoglobin A1c in Type 2 Diabetic Patients
Authors: An Lingwang , Yu Qiuzhi , Chen Linhui , Tang Hong , Liu Yanjun , Yuan Qun , Ji Yu , Lee Yaujiunn , Lu Juming 
Published: January 2022

ObjectiveThis study aimed to explore the relationship between short-term (≤12 months) changes in the estimated glomerular filtration rate (eGFR) and hemoglobin A1c (HbA1c) in patients with type 2 diabetes (T2D).MethodA total of 2,599 patients with T2D were enrolled if they were registered in the Diabetes Sharecare Information System, were aged 18–75 years, and had 2–3 HbA1c and eGFR measurements within the preceding 12 months. The studied patients were categorized into five groups based on eGFR, i.e., the relatively stable (RS), fast decline (FD), modest decline (MD), modest increase (MI), and fast increase (FI) groups.ResultsThe median eGFR changes from baseline were −22.14, −6.44, 0.00, 6.32, and 20.00 ml/min per 1.73 m2 for patients in the FD, MD, RS, MI, and FI groups, respectively. Up to 1,153 (44.4%) subjects experienced an eGFR decline of ≥3.5 ml/min per 1.73 m2, including 821 (31.6%) FD subjects and 332 (12.8%) MD subjects. A decreased trend was found between the eGFR change and HbA1c decrease category, even after multivariable adjustment. In general, an eGFR FD was frequently found in patients who had an HbA1c reduction of ≥3.00% and a baseline HbA1c ≥8.0%; alternatively, such a result was also observed for a urinary albumin-to-creatinine ratio (UACR) of 30.0–300.0 mg/g, regardless of a diabetes duration of &lt;10.0 or ≥10.0 years, or in patients who had an HbA1c reduction of ≥1.00% accompanied by hyperfiltration.ConclusionsSome patients with T2D experienced an eGFR FD or MD during the ≤12-month follow-up period. A significant downward trend in eGFR change was demonstrated alongside an HbA1c reduction, independent of UACR stage, diabetes duration, and hyperfiltration. Sustained monitoring and cautious interpretation of the HbA1c and eGFR changes will be needed in clinical practice.
Efficacy and Safety of Lactobacillus plantarum K50 on Lipids in Koreans With Obesity: A Randomized, Double-Blind Controlled Clinical Trial
Authors: Sohn Minji , Na Ga Yoon , Chu Jaeryang , Joung Hyunchae , Kim Byung-Kook , Lim Soo 
Published: January 2022

BackgroundOnly few studies have investigated the role of probiotics in the development of obesity. We aimed to determine the efficacy and safety of an intake of Lactobacillus plantarum K50 (LPK) on body fat and lipid profiles in people with obesity.MethodsThis randomized, double-blind, placebo-controlled, clinical trial involved 81 adults with a body mass index of 25–30 kg/m2 who were assigned randomly to a diet including 4 × 109 colony-forming unit of LPK or a placebo. Changes in body fat, anthropometric parameters, and biomarkers of obesity were compared using a linear mixed-effect model.ResultsAfter 12 weeks of treatment, body weight, fat mass, and abdominal fat area did not change significantly in the two groups. However, total cholesterol levels decreased from 209.4 ± 34.4 mg/dL to 203.5 ± 30.9 mg/dL in the LPK group, but increased from 194.7 ± 37.5 mg/dL to 199.9 ± 30.7 mg/dL in the placebo group (P = 0.037). Similarly, triglyceride levels decreased from 135.4 ± 115.8 mg/dL to 114.5 ± 65.9 mg/dL in the LPK group, with a significant difference between groups. LPK supplementation also tended to decrease leptin levels compared with placebo. It also changed the distribution of gut microbiota significantly, with an increase in L. plantarum and a decrease in Actinobacteria, both of whose changes in abundance were correlated with changes in visceral adiposity, with borderline significance.ConclusionA 12-week consumption of LPK reduced the total cholesterol and triglyceride levels significantly with favorable alterations in microbiota, suggesting potential benefits for controlling blood lipid profiles.
Prevalence and Trends in Low Bone Density, Osteopenia and Osteoporosis in U.S. Adults With Non-Alcoholic Fatty Liver Disease, 2005–2014
Authors: Zhai Tianyu , Chen Qi , Xu Jing , Jia Xi , Xia Pu 
Published: January 2022

Background &amp; AimsNon-alcoholic fatty liver disease (NAFLD) is suggested to be associated with bone mineral density (BMD) alterations; however, this has not been ascertained. The current study aimed to investigate the changes in BMD and the prevalence of osteopenia/osteoporosis in US adults with or without NAFLD and to evaluate their association.MethodsThe study was conducted based on data collected from the U.S. National Health and Nutrition Examination Survey (NHANES) during the period 2005–2014. A total of 13 837 and 6 177 participants aged &gt; 20 years were eligible for conducting the Hepatic Steatosis Index (HSI) and the US Fatty Liver Index (USFLI) analysis, respectively.ResultsFrom 2005–2014, a downward trend in femoral neck BMD was observed in subjects with NAFLD aged ≥ 40. After adjustment for potential confounders, an upward shift occurred in the prevalence of osteopenia/osteoporosis at the femoral neck in adults aged ≥ 40, particularly in women ≥ 60 years old and men below the age of 60. Moreover, a negative association was found between BMD and NAFLD markers (USFLI, HSI), whereas NAFLD with advanced fibrosis was positively associated with the prevalence of spine fractures.ConclusionsThere was a trend toward lower BMD and higher prevalence of osteopenia/osteoporosis at the femoral neck in US adults with NAFLD aged ≥ 40 years during the period of 2005–2014. NAFLD with advanced fibrosis was positively associated with a higher risk of spine fracture. More research is required to fully investigate the mechanism and consequence of poor bone health in NAFLD patients and consider optimum management of osteopenia/osteoporosis for this population.
The NGS Magic Pudding: A Nanopore-Led Long-Read Genome Assembly for the Commercial Australian Freshwater Crayfish, Cherax destructor
Authors: Austin Christopher M. , Croft Laurence J. , Grandjean Frederic , Gan Han Ming 
Published: January 2022

Cherax destructor, the yabby, is an iconic Australian freshwater crayfish species, which, similar to other major invertebrate groups, is grossly under-represented in genomic databases. The yabby is also the principal commercial freshwater crustacean species in Australia subject to explotation via inland fisheries and aquaculture. To address the genomics knowledge gap for this species and explore cost effective and efficient methods for genome assembly, we generated 106.8 gb of Nanopore reads and performed a long-read only assembly of the Cherax destructor genome. On a mini-server configured with an ultra-fast swap space, the de novo assembly took 131 h (∼5.5 days). Genome polishing with 126.3 gb of PCR-Free Illumina reads generated an assembled genome size of 3.3 gb (74.6% BUSCO completeness) with a contig N50 of 80,900 bp, making it the most contiguous for freshwater crayfish genome assemblies. We found an unusually large number of cellulase genes within the yabby genome which is relevant to understanding the nutritional biology, commercial feed development, and ecological role of this species and crayfish more generally. These resources will be useful for genomic research on freshwater crayfish and our methods for rapid and super-efficient genome assembly will have wide application.
Interkit Reproducibility of the Indirect Immunofluorescence Assay on HEp-2 Cells Depends on the Immunofluorescence Reactivity Intensity and Pattern
Authors: Silva Mônica Jesus , Dellavance Alessandra , Baldo Danielle Cristiane , Rodrigues Silvia Helena , Grecco Marcelle , Prado Monica Simon , Agustinelli Renan , Andrade Luís Eduardo Coelho 
Published: January 2022

IntroductionThe indirect immunofluorescence assay on HEp-2 cells (HEp-2/IFA) is used worldwide for screening for autoantibodies to cellular antigens. Cell culture and fixation methods influence the cell distribution of autoantigens and the preservation of epitopes. Therefore, discrepancy of results obtained using different HEp-2/IFA kits (interkit nonreproducibility) is a common phenomenon in the clinical laboratory routine.ObjectiveThis study evaluated the interkit nonreproducibility of HEp-2/IFA results using samples from patients with systemic autoimmune disease (SAD), nonautoimmune diseases (NAD), and healthy blood donors (HBD).MethodsSerum from 275 SAD patients, 293 NAD patients, and 300 HBD were processed at 1:80 dilution using four HEp-2 kits according to the manufacturers’ instructions. Interkit reproducibility was determined for positive/negative results and patterns. The agreement of positive/negative results among kits for each sample was determined as the reactivity agreement score (RAS). The pattern reproducibility score (PRS) in each sample was calculated as a function of the number of kits showing equivalent patterns. Qualitative variables and ordinal variables were analyzed by the Chi-square and Mann-Whitney U tests, respectively.ResultsA total of 402 samples were nonreactive in all kits and were considered devoid of autoantibodies. Further analysis included the 466 reactive samples (238 SAD, 119 NAD, 109 HBD). Reactivity to the nucleus had the highest interkit reproducibility (RAS = 83.6), followed by the metaphase plate (RAS = 78.9), cytoplasm (RAS = 77.4), and nucleolus (RAS = 72.4). Interkit reproducibility was higher in SAD (RAS = 78.0) than in NAD (RAS = 70.6) and HBD (RAS = 71.3) groups. Samples with strong reactivity (++++/4 and +++/4) had higher interkit reproducibility than those with weak reactivity (+/4). In the SAD group, RAS for nuclear reactivity was 87.5% for strongly reactive samples as opposed to 4.4% for weakly reactive samples, and the same was observed for NAD and HBD samples. The most robust patterns were the centromere AC-3 (PRS = 78.4), multiple nuclear dots AC-6 (PRS = 73.6), nuclear coarse speckled AC-5 (PRS = 71.3), nuclear homogeneous AC-1 (PRS = 67.9), and the reticular cytoplasmic AC-21 (PRS = 68.6).ConclusionInterkit nonreproducibility in HEp-2/IFA is prevalent and occurs with the highest frequency with weakly reactive samples. International initiatives with the engagement of in vitro diagnostic industry are encouraged to promote the harmonization of the properties and performance of HEp-2/IFA commercial kits.
Impact of Periosteal Branches and Septo-Cutaneous Perforators on Free Fibula Flap Outcome: A Retrospective Analysis of Computed Tomography Angiography Scans in Virtual Surgical Planning
Authors: Knitschke Michael , Baumgart Anna Katrin , Bäcker Christina , Adelung Christian , Roller Fritz , Schmermund Daniel , Böttger Sebastian , Streckbein Philipp , Howaldt Hans-Peter , Attia Sameh 
Published: January 2022

BackgroundVirtual surgical planning (VSP) for jaw reconstruction with free fibula flap (FFF) became a routine procedure and requires computed tomography angiography (CTA) for preoperative evaluation of the lower limbs vascular system and the bone. The aim of the study was to assess whether the distribution and density of periosteal branches (PB) and septo-cutaneous perforators (SCP) of the fibular artery have an impact on flap success.MethodThis retrospective clinical study assessed preoperative CTA of the infra-popliteal vasculature and the small vessel system of 72 patients who underwent FFF surgery. Surgical outcome of flap transfer includes wound healing, subtotal, and total flap loss were matched with the segmental vascular supply.ResultA total of 72 patients (28 females, 38.9 %; 44 males, 61.1 %) fulfilled the study inclusion criteria. The mean age was 58.5 (± 15.3 years). Stenoses of the lower limbs’ vessel (n = 14) were mostly detected in the fibular artery (n = 11). Flap success was recorded in n = 59 (82.0%), partial flap failure in n = 4 (5.5%) and total flap loss in n = 9 (12.5%). The study found a mean number (± SD) of 2.53 ± 1.60 PBs and 1.39 ± 1.03 SCPs of the FA at the donor-site. The proximal FFF segment of poly-segmental jaw reconstruction showed a higher rate of PB per flap segment than in the distal segments. Based on the total number of prepared segments (n = 121), 46.7% (n = 7) of mono-, 40.4% (n = 21) of bi-, and 31.5 % (n = 17) of tri-segmental fibula flaps were at least supplied by one PB in the success group. Overall, this corresponds to 37.2% (45 out of 121) of all successful FFF. For total flap loss (n = 14), a relative number of 42.9% (n = 6) of distinct supplied segments was recorded. Wound healing disorder of the donor site was not statistically significant influenced by the detected rate of SCP.ConclusionIn general, a correlation between higher rates of PB and SCP and the flap success could not be statistically proved by the study sample. We conclude, that preoperative PB and SCP mapping based on routine CTA imaging is not suitable for prediction of flap outcome.
Synthesis, Characterization, and in vivo Evaluation of a Novel Potent Autotaxin-Inhibitor
Authors: Hunziker Daniel , Reinehr Sabrina , Palmhof Marina , Wagner Natalie , Biniasch Thomas , Stute Gesa , Mattei Patrizio , Schmitz Petra , DiGiorgio Patrick , Hert Jérôme , Rudolph Markus G. , Benz Joerg , Stihle Martine , Gsell Bernard , Müller Stephan , Gasser Rodolfo , Schonhoven Nina , Ullmer Christoph , Joachim Stephanie C. 
Published: January 2022

The autotaxin-lysophosphatidic acid (ATX-LPA) signaling pathway plays a role in a variety of autoimmune diseases, such as rheumatoid arthritis or neurodegeneration. A link to the pathogenesis of glaucoma is suggested by an overactive ATX-LPA axis in aqueous humor samples of glaucoma patients. Analysis of such samples suggests that the ATX-LPA axis contributes to the fibrogenic activity and resistance to aqueous humor outflow through the trabecular meshwork. In order to inhibit or modulate this pathway, we developed a new series of ATX-inhibitors containing novel bicyclic and spirocyclic structural motifs. A potent lead compound (IC50 against ATX: 6 nM) with good in vivo PK, favorable in vitro property, and safety profile was generated. This compound leads to lowered LPA levels in vivo after oral administration. Hence, it was suitable for chronic oral treatment in two rodent models of glaucoma, the experimental autoimmune glaucoma (EAG) and the ischemia/reperfusion models. In the EAG model, rats were immunized with an optic nerve antigen homogenate, while controls received sodium chloride. Retinal ischemia/reperfusion (I/R) was induced by elevating the intraocular pressure (IOP) in one eye to 140 mmHg for 60 min, followed by reperfusion, while the other untreated eye served as control. Retinae and optic nerves were evaluated 28 days after EAG or 7 and 14 days after I/R induction. Oral treatment with the optimized ATX-inhibitor lead to reduced retinal ganglion cell (RGC) loss in both glaucoma models. In the optic nerve, the protective effect of ATX inhibition was less effective compared to the retina and only a trend to a weakened neurofilament distortion was detectable. Taken together, these results provide evidence that the dysregulation of the ATX-LPA axis in the aqueous humor of glaucoma patients, in addition to the postulated outflow impairment, might also contribute to RGC loss. The observation that ATX-inhibitor treatment in both glaucoma models did not result in significant IOP increases or decreases after oral treatment indicates that protection from RGC loss due to inhibition of the ATX-LPA axis is independent of an IOP lowering effect.
Computational and Biological Evaluation of β-Adrenoreceptor Blockers as Promising Bacterial Anti-Virulence Agents
Authors: Almalki Ahmad J. , Ibrahim Tarek S. , Elhady Sameh S. , Hegazy Wael A. H. , Darwish Khaled M. 
Published: January 2022

Bacterial resistance to antibiotics is an increasing public health threat as it has the potential to affect people at any stage of life, as well as veterinary. Various approaches have been proposed to counteract the bacterial resistance development. Tackling bacterial virulence is one of the most promising approaches that confer several merits. The bacterial virulence is mainly regulated by a communication system known as quorum sensing (QS) system. Meanwhile, bacteria can sense the adrenergic hormones and eavesdrops on the host cells to establish their infection, adrenergic hormones were shown to enhance the bacterial virulence. In this study, β-adrenoreceptor blockers were proposed not only to stop bacterial espionage on our cells but also as inhibitors to the bacterial QS systems. In this context, a detailed in silico study has been conducted to evaluate the affinities of twenty-two β-blockers to compete on different structural QS receptors. Among the best docked and thermodynamically stable β-blockers; atenolol, esmolol, and metoprolol were subjected to further in vitro and in vivo investigation to evaluate their anti-QS activities against Chromobacterium violaceum, Pseudomonas aeruginosa and Salmonella typhimurium. The three tested β-blockers decreased the production of QS-controlled C. violaceum, and the formation of biofilm by P. aeruginosa and S. typhimurium. Additionally, the tested β-blockers down-regulated the P. aeruginosa QS-encoding genes and S. typhimurium sensor kinase encoding genes. Furthermore, metoprolol protected mice against P. aeruginosa and S. typhimurium. Conclusively, these investigated β-blockers are promising anti-virulence agents antagonizing adrenergic hormones induced virulence, preventing bacterial espionage, and blocking bacterial QS systems.