bluuebunny/crossref_metadata_2025_split huggingface dataset:
Specificity and Confirmation of SARS-CoV-2 Serological Test Methods in Emergency Department Populations across the United States
Authors: Daghfal David J , Schneider Randal J , Mohr Phaedre , Frias Edwin C , Prostko John C , Sokoll Lori J 
Published: June 2022

Abstract

Background
Serological testing for SARS-CoV-2 is integral for understanding prevalence of disease, tracking of infections, confirming humoral response to vaccines, and determining timing and efficacy of boosters. The study objective was to compare the specificity of serology assays in emergency department populations across the United States in 2019 (pre-pandemic) and early 2020, incorporating an automated confirmatory assay.

Methods
Patient specimens (n = 1954) were from 4 regions in the United States: New York, NY; Milwaukee, WI; Miami, FL; and Los Angeles, CA. Specimens were tested with SARS-CoV-2 anti-spike receptor-binding domain assays: SARS-CoV-2 IgG on the Abbott Alinity i (AdviseDx SARS-Cov-2 IgG II) and Beckman Coulter Access 2 (SARS-CoV-2 IgG II), and SARS-CoV-2 IgM on the Abbott Alinity i (AdviseDx SARS-CoV-2 IgM). Reactive samples were tested with a research use only angiotensin-converting enzyme 2 binding inhibition assay (Abbott ARCHITECT) for confirmation of SARS-CoV-2 neutralizing antibodies. Assay specificity was determined and comparisons performed with Fisher’s exact test.

Results
Overall SARS-CoV-2 IgG specificity was 99.28% (95% confidence interval, 98.80%–99.61%), 99.39% (98.93%–99.68%), and 99.44% (98.99%–99.72%) for SARS-CoV-2 IgG by Abbott and Beckman, and SARS-CoV-2 IgM, respectively. Overall agreement for the two IgG assays was 99.28% (range for the 4 sites: 98.21% to 100%). There were no specificity differences between assays or sites.

Conclusions
The specificity of the serological assays evaluated in a large, diverse emergency department population was &gt;99% and did not vary by geographical site. A confirmatory algorithm with an automated pseudo-neutralization assay allowed testing on the same specimen while reducing the false positivity rate and increasing the value of serology screening methods.
Growth performance, antibody response, and mammary gland development in New Zealand dairy replacement bovine heifers fed low or high amounts of unpasteurized whole milk
Authors: Khan M Ajmal , Heiser Axel , Maclean Paul H , Leath Shane R , Lowe Katherine A , Molenaar Adrian J 
Published: June 2022

Abstract
This study evaluated the influence of feeding low and high preweaning allowances of unpasteurized whole milk (MA) on intake, selected blood metabolites, antibody response, mammary gland growth, and growth of New Zealand (NZ) dairy heifers to 7 mo of age. At 10 ± 2 d of age (study day 0), group-housed (six·pen−1) heifer calves (Holstein-Friesian × Jersey) were allocated to low (4 L whole milk·calf−1·d−1; n = 7 pens) or high (8 L whole milk·calf−1·d−1; n = 7 pens) MA for the next 63 d. Calves were gradually weaned between days 63 ± 2 and 73 ± 2. Calves in each pen had ad-libitum access to clean water, pelleted calf starter, and chopped grass hay from day 1 to 91 ± 2 d. At 92 ± 2 d, all calves were transferred to pasture, grazed in a mob, and their growth and selected blood metabolites were measured until day 209. All animals were weighed weekly during the indoor period (to day 91) and then at days 105, 112, 128, 162, 184, and 209. Skeletal growth measurements and blood samples to analyze selected metabolites were collected at the start of the experiment, weaning, and then postweaning on day 91, and day 201. Specific antibodies against Leptospira and Clostridia were quantified in weeks 7, 13, and 27. Mammary glands were scanned using ultrasonography at the start of the experiment, weaning, and day 201. Feeding high vs. low amounts of MA increased the preweaning growth in heifer calves (P = 0.02) without negatively affecting postweaning average daily gain (ADG) (P = 0.74). Compared with heifers fed with low MA, high MA fed heifers had a greater increase in antibodies against Leptospira and Clostridia by 13 wk of age (P = 0.0007 and P = 0.06, respectively). By 27 wk of age, the antibody response was the same in heifers offered low or high MA. There was no effect of MA on the total size of the mammary gland, measured by ultrasonography, at weaning and 7 mo of age. However, the greater MA was associated with more mammary parenchyma (P = 0.01) and less mammary fat pad (P = 0.03) in back glands at 7 mo of age compared with heifers fed lower MA. In conclusion, feeding a high vs. a low amount of unpasteurized whole milk increased the preweaning growth of New Zealand replacement heifers without negatively affecting their ADG during postweaning under grazing conditions. Feeding more (8 vs. 4 L·d−1) unpasteurized whole milk positively affected antibody responses early in life and mammary gland composition by 7 mo of age in dairy heifers reared for pasture-based dairy systems.
John Brown’s Description of the Lake at Keswick: A Manuscript and a Few More Clues
Authors: Donaldson Christopher 
Published: June 2022

Abstract
This note reports on a previously undocumented manuscript copy of John Brown’s Description of the Lake at Keswick. Brown’s Description is an early, influential account of Derwent Water in the English Lake District. The text is generally regarded as having helped promote picturesque tourism in the region during the later eighteenth century. Prior to publication, the Description circulated privately. Until recently, however, none of these private copies were known to exist. The provenance of the manuscript discussed in this note is obscure, but there are grounds for supposing that the manuscript may relate to copies of Brown’s Description published in Newcastle upon Tyne, UK, during the mid-1760s. The note outlines the evidence for this potential relationship and, in doing so, puts a few more pieces of the puzzling history of Brown’s Description into place.
The Authorship of The Occasional Paper (London, 1697–98)
Authors: Walmsley J C , Antonia Alexis , Craig Hugh 
Published: June 2022

Abstract
The Occasional Paper was a series of anonymous pamphlets covering a diverse range of topics, published in ten numbers during 1697 and 1698. The series has always been attributed to Richard Willis (bap. 1664, d. 1734), successively bishop of Gloucester (1715–21), Salisbury (1721–23), and Winchester (1723–34). Edward Waddington’s (1670?–1731) copy of The Occasional Paper provides near-contemporary attributions of nine of the numbers to the clergymen William Hayley (1657–1715), Gilbert Burnet (1643–1715), Richard Lucas (1648/9–1715), Charles Trimnell (1663–1723), Samuel Bradford (1652–1731), William Hodges (1667–1703), and Willis. These attributions are consistent with the content of The Occasional Paper, and with a stylometric analysis of texts known to be by the attributed authors. This demonstrates that it was William Hayley that criticized Locke’s Reasonableness of Christianity (1695) in the first and fifth of these numbers, not Willis. It also reveals that Number II, ‘Concerning the Late Unfortunate Death of J. H----en, Esq’, presents the contemporary deathbed confession of the politician John Hampden (bap. 1653, d. 1696), communicated by Gilbert Burnet.
The first-in-human phase I study of a brain-penetrant mutant IDH1 inhibitor DS-1001 in patients with recurrent or progressive IDH1-mutant gliomas
Authors: Natsume Atsushi , Arakawa Yoshiki , Narita Yoshitaka , Sugiyama Kazuhiko , Hata Nobuhiro , Muragaki Yoshihiro , Shinojima Naoki , Kumabe Toshihiro , Saito Ryuta , Motomura Kazuya , Mineharu Yohei , Miyakita Yasuji , Yamasaki Fumiyuki , Matsushita Yuko , Ichimura Koichi , Ito Kazumi , Tachibana Masaya , Kakurai Yasuyuki , Okamoto Naoko , Asahi Takashi , Nishijima Soichiro , Yamaguchi Tomoyuki , Tsubouchi Hiroshi , Nakamura Hideo , Nishikawa Ryo 
Published: June 2022

Abstract

Background
Approximately 70% of lower-grade gliomas harbor isocitrate dehydrogenase 1 (IDH1) mutations, resulting in the accumulation of oncometabolite D-2-hydroxyglutarate (D-2-HG); this leads to epigenetic dysregulation, oncogenesis, and subsequent clonal expansion. DS-1001 is an oral brain-penetrant mutant IDH1 selective inhibitor. This first-in-human study investigated the safety, pharmacokinetics, pharmacodynamics, and efficacy of DS-1001.

Methods
This was a multicenter, open-label, dose-escalation, phase I study of DS-1001 for recurrent/progressive IDH1-mutant (R132) glioma (N = 47) (NCT03030066). DS-1001 was administered orally at 125-1400 mg twice daily. Dose-escalation used a modified continual reassessment method.

Results
The maximum tolerated dose was not reached. Eight patients were continuing treatment at the data cutoff. Most adverse events (AEs) were grade 1-2. Twenty patients (42.6%) experienced at least 1 grade 3 AE. No grade 4 or 5 AEs or serious drug-related AEs were reported. Common AEs (&gt;20%) were skin hyperpigmentation, diarrhea, pruritus, alopecia, arthralgia, nausea, headache, rash, and dry skin. The objective response rates were 17.1% for enhancing tumors and 33.3% for non-enhancing tumors. Median progression-free survival was 10.4 months (95% confidence interval [CI], 6.1 to 17.7 months) and not reached (95% CI, 24.1 to not reached) for the enhancing and non-enhancing glioma cohorts, respectively. Seven on-treatment brain tumor samples showed a significantly lower amount of D-2-HG compared with pre-study archived samples.

Conclusions
DS-1001 was well tolerated with a favorable brain distribution. Recurrent/progressive IDH1-mutant glioma patients responded to treatment. A study of DS-1001 in patients with chemotherapy- and radiotherapy-naïve IDH1-mutated WHO grade 2 glioma is ongoing (NCT04458272).
Loss of SQSTM1/p62 Induces Obesity and Exacerbates Alcohol‐induced Liver Injury in Aged Mice
Authors: Qian Hui 
Published: June 2022

BackgroundAlcohol‐related liver disease (ALD) is a worldwide health problem without successful treatment. Aging is associate with obesity, which are known a risk factor that aggravate ALD. We and others have previously demonstrated that chronic plus binge alcohol increases adipose tissue lipolysis and promotes alcohol‐induced liver injury through the adipose‐liver crosstalk. In the present study, we investigated the role of autophagy receptor protein SQSTM1/p62 in adipose‐liver crosstalk and the effects of aging and obesity in ALD.MethodsWhole‐body p62 knockout (KO) and their matched wild type young (2‐3 months) and aged (13‐15 months) mice were subjected to chronic plus binge (Gao‐binge) alcohol model. Adipose and liver tissues were collected for biochemical and histological analysis.Resultsp62 KO mice developed mature‐onset obesity in aged mice with increased both white and brown adipose tissue mass. Gao‐binge alcohol feeding decreased both the white and adipose mass regardless of the age and genotypes of mice. Alcohol feeding increased levels of serum free fatty acids regardless of the genotypes of the mice although the serum levels of glycerol only significantly increased in alcohol fed aged p62 KO mice. Alcohol feeding increased levels of serum ALT and hepatic triglyceride (TG) in both young and aged WT and p62 KO mice, but no significant differences were found among the young WT and p62 KO mice as well as aged WT vs young WT mice. However, alcohol‐fed aged p62 KO mice had significantly higher levels of serum ALT and hepatic TG as well as fibrosis and inflammation markers than alcohol‐fed aged WT mice. Mechanistically, aged p62 KO mice had increased de novo lipogenesis and senescence with increased senescence associated secretory phenotype with similar increased oxidative stress compared with matched WT mice.ConclusionWe established a genetic obesity/metabolic syndrome mouse model with alcohol consumption. Loss of p62 in aged mice leads to obesity with metabolic syndrome that exacerbates alcohol‐induced liver injury via increased de novo lipogenesis and senescence.
Limb Salvage with Multiple Modalities: A Case Report of a Diabetic Heel Ulcer Associated with Peripheral Arterial Disease
Authors: Matsuzaki Kyoichi , Miyamoto Akira 
Published: June 2022

ABSTRACT
A large diabetic heel ulcer with peripheral arterial disease is an independent predictor of limb loss; below-knee amputation is not uncommon in such cases. One treatment is multimodal therapy, which includes partial calcanectomy. Because there is a limit to the ulcer surface area that can be sutured after partial calcanectomy, the remaining raw surface is treated with another method. In this case report, the authors describe a patient with peripheral arterial disease who had a 7 × 9-cm diabetic heel ulcer. The patient was treated with partial calcanectomy after catheter-based endovascular therapy revascularization and then maggot therapy after residual-wound dimensions were reduced by negative-pressure wound therapy.
Successful Treatment of Wound Dehiscence by Innovative Type 1 Collagen Flowable Gel: A Case Report
Authors: Ferraro Giuseppe A. , Lanzano Giuseppe , Grella Elisa , Gubitosi Adelmo , Nicoletti Giovanni F. 
Published: June 2022

Summary:
The growing demand for postbariatric body-contouring surgery after massive weight loss goes hand-in-hand with an increase in wound complications. Consequently, surgical reoperation or conservative management is necessary and represents a difficult challenge to healthcare professionals. Moreover, it is well known that postbariatric patients present aberrant wound healing due to multifactorial causes, such as preoperative illness, nutritional deficiencies, and vascular disease. To treat such complex wounds, several methods have been recommended, such as the use of negative pressure wound therapy, tissue-engineered skin substitutes, and collagen-based wound dressings. The case presented here is of a patient with deep wound dehiscence of the inner left thigh, 1 week after a medial thigh lift procedure, successfully managed with Vergenix Flowable Gel, a human recombinant type I collagen produced in plants. After 2 weeks of treatment, wound dehiscence was replaced with granulation tissue, and after 4 weeks, the patient was completely healed, with an acceptable aesthetic outcome of the surgical scar.
Treatment of Delayed-onset Inflammatory Reactions to Hyaluronic Acid Filler: An Algorithmic Approach
Authors: Funt David K. 
Published: June 2022

Summary:
Hyaluronic acid fillers are one of the most widely used and versatile fillers worldwide. Although traditionally regarded as immunologically inert, many currently available products have been substantially modified to improve longevity and to optimize properties for specific indications. Such modifications, either alone or in combination with other factors (such as the immune status of the patient, immune-triggering events, and bacterial contamination), may lead to the development of late-onset inflammatory nodules in some patients. This article discusses the clinical presentation of late-onset adverse inflammatory reactions to hyaluronic acid injections, describes their likely triggers, and presents the author’s treatment algorithm for successful resolution.
Cost and Satisfaction Implications of Using Telehealth for Plagiocephaly
Authors: Cappitelli Alex , Wenzinger Eric , Langa Olivia C. , Nuzzi Laura , Ganor Oren , Rogers-Vizena Carolyn R. , Ganske Ingrid M. 
Published: June 2022

Purpose:
Patients with deformational plagiocephaly are often referred for evaluation by a plastic surgeon. During the early COVID-19 pandemic, visits were performed predominantly via telehealth. This study compares costs, satisfaction, and technological considerations for telehealth and in-person consultations for plagiocephaly.

Methods:
This prospective study evaluated telehealth and in-person consultation for plagiocephaly between August 2020 and January 2021. Costs were estimated using time-driven activity-based costing (TDABC) and included personnel and facility costs. Patient-borne expenses for travel were assessed. Post-visit questionnaires administered to patients’ families and providers measured satisfaction with the consult and technical issues encountered.

Results:
Costing analysis was performed on 20 telehealth and 11 in-person consults. Median total personnel and facility costs of providing in-person or telehealth consults were comparable (P > 0.05). Telehealth visits saved on the cost of clinic space but required significantly more of the provider’s time (P < 0.05). In-person visits had an additional patient-borne travel cost of $28.64. Technical difficulties were reported among 25% (n = 5) of telehealth consults. Paired provider and patient experience questionnaires were collected from 17 consults (11 telehealth, six in-person). Overall satisfaction with care did not differ significantly between consult types or between the provider and patient family (P > 0.05).

Conclusions:
Costs of providing in-person and telehealth plagiocephaly consultations were comparable, whereas patients incur greater costs when coming in person. Practices that treat patients with plagiocephaly may wish to consider expanding their virtual consult offerings to families desiring this option. Long-term outcome studies are necessary to evaluate the efficacy of both visit types.
Gender-affirming Phalloplasty: A Postoperative Protocol for Success
Authors: Rifkin William J. , Daar David A. , Cripps Courtney N. , Mars Ginger , Zhao Lee C. , Levine Jamie P. , Bluebond-Langner Rachel 
Published: June 2022

Background:
Increased access to care and insurance coverage has led to an increase in gender-affirming surgeries performed in the United States. Gender-affirming phalloplasty has a variety of donor sites and surgical techniques including both pedicled and free flaps. Although surgical techniques and patient outcomes are well-described, no reports in the literature specifically discuss postoperative management, which plays a crucial role in the success of these operations. Here, we present a postoperative protocol based on our institution’s experience with gender-affirming phalloplasty with the hope it will serve as a standardized, reproducible reference for centers looking to offer these procedures.

Methods:
Patients undergoing gender-affirming phalloplasty at our institution followed a standardized protocol from the preoperative stage through phases of postoperative recovery. Medication, laboratory, physical and occupational therapy, flap monitoring, and dressing change guidelines were extracted and compiled into a single resource detailing the postoperative protocol in full.

Results:
Our institution's standardized postoperative protocol for gender-affirming phalloplasty is detailed, focusing on flap monitoring, mobilization and activity, medications, and postoperative dressing care. One hundred thirty first-stage phalloplasty procedures were performed between May 2017 and December 2021, with two patients (1.5%) experiencing partial necrosis and one incidence (0.8%) of total flap loss.

Conclusions:
For optimal and safe surgical outcomes, the surgical and extended care teams need to understand flap monitoring as well as specific postoperative protocols. A systematic approach focusing on flap monitoring, mobilization and activity, medications, and postoperative dressing care decreases errors, accelerates recovery, shortens length of stay, and instills confidence in the patient.
Multihit Injury of the Radial Nerve in a 62-year-old Woman: A Case Report
Authors: Heinzel Johannes C. , Winter Natalie , Stahl Jan-Hendrik , Prahm Cosima , Grimm Alexander , Kolbenschlag Jonas 
Published: June 2022

Summary:
We report the case of a 62-year-old female patient with a triple-crush radial nerve injury, diagnosed in subsequent order following a fracture of the left humerus. The patient developed flaccid paralysis of all muscles innervated by the left radial nerve except the triceps brachii and reported a sensory deficit corresponding to the innervation territories of the posterior nerve of the forearm as well as the superficial branch of the radial nerve. Following neurolysis of the radial nerve at the humerus level, wrist extension as well as sensory perception on the dorsal aspect of the forearm recovered, but finger extension and thumb abduction were still impossible. Following neurological evaluation and nerve ultrasound, supinator syndrome was diagnosed and the patient underwent decompression surgery. Following surgical decompression, motor recovery was observable but a sensory deficit remained in the area innervated by the superficial branch of the radial nerve. In consequence, the third crush injury of the left radial nerve, that is, Wartenberg syndrome or cheiralgia paraesthetica was diagnosed. Decompression surgery of the superficial branch of the radial nerve was performed and the patient reported profound amelioration of her sensory symptoms during a follow-up examination at our outpatient clinic 6 weeks postoperatively.
Plausibility of Claimed Covid-19 Vaccine Efficacies by Age: A Simulation Study
Authors: Sheldrick Kyle A. , Meyerowitz-Katz Gideon , Tucker-Kellogg Greg 
Published: June 2022

Background:
Multiple vaccines against Covid-19 have passed through phase-3 trials; however, concerns have been raised about alleged excessive similarity of efficacy across age groups for the Sputnik V vaccine.

Study Question:
How likely are the observed efficacies for all age subgroups to fall within the range of by-age efficacies claimed for the AstraZeneca, Janssen, Moderna, Pfizer, and Sputnik V vaccines, assuming that there is no effect of age on efficacy?

Study Design:
We performed a simulation study using R of 1000 and then 50,000 simulated trials for each vaccine, with random allocation to each arm but fixed enrollment numbers by age group. We used study-wide efficacy and infection rate for all age groups. We recorded the observed vaccine efficacies in each age group and summated how many simulations had all observed efficacies fall within the range of efficacies described in the relevant article.

Results:
In the 1000-trial simulation for the AstraZeneca vaccine, in 23.8% of simulated trials, the observed efficacies of all age subgroups fell within the efficacy bounds for age subgroups in the published article. The J + J simulation showed 44.7%, Moderna 51.1%, Pfizer 30.5%, and 0.0% of the Sputnik simulated trials had all age subgroups fall within the limits of the efficacy estimates described by the published article. In 50,000 simulated trials of the Sputnik vaccine, 0.026% had all age subgroups fall within the limits of the efficacy estimates described by the published article, whereas 99.974% did not.

Conclusions:
The distribution of alleged vaccine efficacies of the Sputnik vaccine by age in the phase-III trial is very unlikely to occur in genuine experimental data, even if the number of patients recruited, vaccine efficacy, and overall infection rate are true and there is no underlying difference in vaccine efficacy by age.