bluuebunny/crossref_metadata_2025_split huggingface dataset:
Operation Range Enhancement for Alkaline Electrolysers Driven by Renewable Energy Sources
Authors: Xia Yanghong , Cheng Haoran , He Hanghang , Wei Wei 
Published: November 2022

AbstractLow-cost and mature alkaline water electrolysis is suitable for large-scale hydrogen production from renewable energy sources (RESs). However, the poor low-load performance of alkaline water electrolysers (AWEs) makes it difficult to follow fluctuant RESs in full range. For low-load AWEs, existing researches only pay attention to the impurity problem and its solutions, but here, we find other two urgent problems to be solved, namely inefficiency and inconsistency. Through the detailed operation process analysis of AWEs and the established equivalent electrical model, the inefficiency and inconsistency mechanisms of low-load AWEs are revealed. Furthermore, an overcurrent pulse-width modulation method is proposed to enhance the efficiency and consistency of AWEs. Especially, compared to the conventional dc power supply, 1) the maximum efficiency improvement can exceed two times, 2) the operation range can be extended to 10% of rated load. The proposed method just changes the power supply, it can be easily generalized and can facilitate the hydrogen production from RESs.
MOLECULAR DYNAMICS SIMULATION OF FOUR BIFLAVONOIDS WITH THE LEISHMANOLYSIN (gp63) PROTEIN OF Leishmania major
Authors: Mercado-Camargo Jairo , Cervantes-Ceballos Leonor , Vivas-Reyes Ricardo , Olmo-Fernández Esther , Gómez-Estrada Harold 
Published: November 2022

Abstract

Leishmanolisyn (gp63) is a 63 kDa surface metalloprotease belonging to the M8 family (subclan MA (M), metzincines) that is found in both promastigote and amastigote ofleishmania ssp. It constitutes one of the virulence factors in the pathogenesis of Leishmania spp., being crucial in its entry into the macrophage and in complement activation. Therefore, gp63 may be a therapeutic alternative for potential new drugs needed to combat this disease. Among the many functional groups of naturally occurring compounds, the biflavonoids amentoflavone (A), lanaraflavone (L), podocarpusflavone A (PA), and podocarpusflovone B (PB) are promising candidate ligands for the treatment of all forms of leishmaniasis. In this study we carried out the validated molecular dynamics simulation with GROMACS, using mean square deviation (RMSD), mean square fluctuation (RMSF), the radius of gyration (Rg), and intermolecular hydrogen bonds (H-bonds) between the gp63 protein ofL. major(1LML) in complex with the four biflavonoids. In addition, the free binding energies were estimated using the MM-PBSA method, to determine their stability in the active site of the protein. The computational analysis revealed that the interaction of lanaroflavone (ΔGBing= -156.75 ± 31.91 Kcal/mol forL. major) remained stable in this enzyme, revealing their remarkable potential as a possible antileishmanial agent to combat the Leishmania parasite. On the other hand, amentoflavone, podocarpusflavone A, and B also showed a good affinity for both metalloproteases according to the ΔGbindvalues obtained.
A noval high-order discretization method for the milling stability prediction considering the differential of directional cutting coefficient and vibration velocities
Authors: Song Chunlei 
Published: November 2022

Abstract
A novel high-order discretization method for the prediction of milling stability is proposed in this paper to increase the accuracy and efficiency considering the differential of directional cutting coefficient and vibration velocities. Same to the existing full discretization method (FDM) and semi discretization method (SDM), the milling system is expressed as a linear time-periodic equation and the time period is discretized into discrete time intervals to approximate the solution. In this algorithm, the cutting force coefficient matrix of the whole integrand is reconstructed to lay the foundation for the fast and accurate approximation. Then, the monodromy matrix (or the Floquet matrix) is calculated by the method used in the temporal finite element analysis (TFEA) instead of the multiple recursive algorithms which can improve the computational time. Finally, the computational efficiency is defined in a new way which gives quantitative comparisons for different discretization methods. It is shown that proposed method can reduce the computational cost by 91–95% when the same errors are required .
Effect of Lithium Administration on Brain Activity Under an Emotion Regulation Paradigm in Healthy Participants: A Functional Magnetic Resonance Imaging Study
Authors: Hortelano Pilar Artiach , Martens Marieke A G , Pringle Abigail , Harmer Catherine J 
Published: November 2022

Abstract

Rationale:
Emotion Regulation (ER) difficulties have been previously described in bipolar disorder (BD). Whilst lithium has been shown to be effective in the treatment of BD, the mechanisms underlying lithium’s effect on mood stabilisation remain unclear.
Objectives
Unravelling lithium’s effect on psychological processes impaired in BD, such as ER, could address this translational gap and inform the development of new treatments.
Methods
This study investigated the neural effects of lithium on ER in 36 healthy volunteers in a double-blind between-groups design, with randomised administration of lithium or placebo for 11 days. At treatment completion, participants underwent 3- Tesla functional magnetic resonance imaging while performing an ER task. Participants viewed negative images and were instructed to maintain or reappraise the elicited emotions and rate the negative affect experienced.
Results
During reappraisal (versus maintain), lithium increased activity in the right middle temporal gyrus (MTG, Z > 3.1, p < 0.05 corrected) and decreased activation in the left angular gyrus, the left anterior prefrontal cortex (or rostral PFC, BA10) and right superior frontal gyrus (Z > 2.3, p < 0.05 corrected). In addition, greater connectivity was found between the right MTG and left middle frontal gyrus for the lithium group compared to placebo, during reappraisal (versus maintain) (Z > 2.3, p < 0.05 corrected). Further effects of lithium were found in response to negative picture presentation, whereby decreased connectivity was found between the left amygdala and the frontal cortex, and greater connectivity between the right MTG and the bilateral medial prefrontal cortex extending into the paracingulate gyrus, compared to placebo (Z > 2.3, p < 0.05 corrected).
Conclusions
These results show a potential effect of lithium on ER through its effects on activity and connectivity, and further elaborate the neural underpinnings of cognitive reappraisal. Future work should investigate more extended effects of lithium on ER, ultimately benefitting the development of novel and more effective treatments for BD.
Cryptic cycles of microbial hydrocarbon production and degradation along freshwater-saltwater ecosystem gradients
Authors: Vigneron Adrien , Cruaud Perrine , Lovejoy Connie , Vincent Warwick 
Published: November 2022

AbstractCyanobacteria produce vast quantities of long-chain alkanes in the ocean, yet these do not accumulate in the water column, suggesting rapid co-localized biodegradation. The identities of microbes in this cryptic hydrocarbon cycle are mostly unknown, and are unexplored across marine-freshwater gradients. Analyzing genes and metagenome assembled genomes from a remote, pristine, petroleum-free and meromictic lake in the High Arctic, we detected microbial hydrocarbon production and degradation pathways at all depths, from surface freshwaters to dark, saline, anoxic waters. In addition to Cyanobacteria, members of the phyla Flavobacteria, Nitrospina, Deltaproteobacteria, Planctomycetes and Verrucomicrobia had pathways for hydrocarbon production, providing additional sources of biogenic hydrocarbons. Classic oil-degrading microorganisms were poorly represented in the system, while long-chain hydrocarbon degradation genes were identified in various freshwater and marine lineages such as Actinobacteria, Schleiferiaceae and Marinimicrobia. This suggests that biogenic hydrocarbons could sustain a large fraction of freshwater and oceanic microbiomes, with global biogeochemical implications.
Case Report: Video-Assisted Thoracoscopic Surgery for Pulmonary Arteriovenous Malformation Using Near-Infrared Fluorescence with Indocyanine Green
Authors: Han Tianxiao , Gao Jian , Wang Zhenfan , Zhou Jian 
Published: November 2022

Abstract
Background: Pulmonary arteriovenous malformation (PAVM) is an abnormal communication between pulmonary vasculatures and has an unclear boundary with surrounding lung tissues. At present, surgeons can only determine its location by preoperative imaging and intraoperative palpation, despite its soft texture. Indocyanine green, a near-infrared fluorophore, has been demonstrated useful in the accurate identification of vascular tissue. Therefore, we explored its application in PAVM cases.
Case presentation: we present two PAVM cases using near-infrared fluorescence (NIF) with 25 mg ICG at 5 mg/ml to achieve intraoperative visualization of the lesion in video-assisted thoracoscopic surgery (VATS), with one for relieving symptoms and the other for complicated lung disease requiring surgery. Under the NIF mode, ICG systemic injection led to successive signaling of the anomaly and normal tissues in merely 10 seconds, which helped us distinguish them efficiently and precisely. A peak signal-to-background ratio of 2.2 confirmed the significant fluorescence difference as well and excluded interference from carbon dust furthermore.
Conclusions:We are the first to report the use of such an approach in delineating the margin of vascular malformation with high contrast and this new finding may be helpful to minimize the damage to lung function in PAVM treatment. Further exploration and validation are needed to determine its role.
A Causal Analysis of Financial Development and Economic Growth
Authors: Islam Tanweer Ul , Ahmad Muneeb-ud-din 
Published: November 2022

Abstract
In terms of cause and effect, financial development and economic growth relationship has been a major empirical issue for researchers and policy makers. This study explores the causal linkages between financial development and economic growth catering for country specificity and structural shocks pertaining to different cross sections in SAARC and ASEAN countries by calling in the bootstrapping technique proposed by Kónya (2006). We considered the panel estimation to allow for contemporaneous correlations and performed Wald test for Granger Causality with country specific bootstrap critical values catering for structural breaks. Empirical investigation reveals the existence of both the demand and supply leading phenomena for the selected period of 1980–2017. The direction of causality is sensitive measures and proxies used for financial development.
Discovery of pentacyclic triterpenoid saponins with anti-proliferative activities from Ardisia lindleyana
Authors: Wei Zhen-Zhen , Zhou Ti-Qiang , Zhang Jing-Rui , Xia Zi-Ming , Liu Si-Fan , Liu Chun-Ying , Li Min , Zhang Guang-Jie , Tian Ying , Li Bin , Wang Lin , Liu Shu-Chen 
Published: November 2022

Abstract
One new pentacyclic triterpenoid saponin, ardisiapunine E (1), along with two known compounds were isolated from the root of Ardisia lindleyana D.Dietr. Their structures were elucidated by 1H and 13C NMR, DEPT, HMBC, HSQC, 1H-1H COSY and NOESY spectroscopic analyses, ESI-MS, and literature. Compounds 1-3 exhibited obvious anti-proliferative activities against HeLa cell line in a dose- and time-dependent manner through inducing G2/M phase arrest and apoptosis in vitro, both consisting of pentacyclic triterpenes and sugar. Hence, this study identified a new and two known pentacyclic triterpenoid saponin promoting apoptosis as a potential therapeutic option for cancer.
Molecular dynamics simulation of mechanical properties of carbon nanotube reinforced cellulose
Authors: Li Kecheng , Qi DEWEI 
Published: November 2022

Abstract
Cellulose, hemicellulose, and lignin are the major chemical components in wood paper. Various types of wet and dry strength additives are used to enhance the optical and mechanical properties of recycled paper. One of possible materials is carbon nanotube. In order to explore probability of use of carbon nanotubes as reinforcing materials and to understand how carbon nanotubes affect the mechanical properties of paper, a single walled carbon nanotube is inserted into a Iβ cellulose nanocrystal, its mechanical properties are studied by using energy minimization and molecular dynamics (MD) simulations. During simulations, the crystals are stretched in axial direction at a constant speed and stress and strain are computed and recorded at atomic level. Our results show that carbon nanotube can significantly enhance mechanical properties of paper.
Association of full premedication on tracheal intubation outcomes in the neonatal intensive care unit: an observational cohort study
Authors: Neches Sara , Brei Brianna , Umoren Rachel , Gray Megan , Nishisaki Akira , Foglia Elizabeth , Sawyer Taylor 
Published: November 2022

Abstract
Objective: Evaluate the association of short-term tracheal intubation (TI) outcomes with premedication in the NICU.
Study Design: Observational single-center cohort study comparing TIs with full premedication (opiate analgesia and vagolytic and paralytic), partial premedication, and no premedication. The primary outcome is adverse TI associated events (TIAEs) in intubations with full premedication compared to those with partial or no premedication. Secondary outcomes included change in heart rate and first attempt TI success.
Results: 352 encounters in 253 infants (median gestation 28 weeks, birth weight 1100g) were analyzed. TI with full premedication was associated with fewer TIAEs aOR 0.26 (95%CI 0.1-0.6) compared with no premedication, and higher first attempt success aOR 2.7 (95%CI 1.3-4.5) compared with partial premedication after adjusting for patient and provider characteristics.
Conclusion: The use of full premedication for neonatal TI, including an opiate, vagolytic, and paralytic, is associated with fewer adverse events compared with no and partial premedication.
A large special posterior mitral valve aneurysm without infective endocarditis and literature review
Authors: Hu Wan Yu , Wang Bei , Zhao Bo-wen , Li Shi-yan 
Published: November 2022

Abstract
Background

Mitral valve aneurysm (MVA) was a saccular bulging on mitral valves. It often occurred in anterior leaflet and commonly caused by aortic valve regurgitation complicated by infective endocarditis.

Case presentation

We reported a 68-year-old female with MVA of posterior leaflet without infective endocarditis. The aneurysm was large and the characteristics of its echo imaging was special, because the saccular structure did not collapse during diastole in real time three dimentional transesophageal echocardiography (RT-3D-TEE). But direct communication between the mass and the left ventricle was detected in this patient. This jet flow from left ventricle into the aneurysm help us distinguish MVA from mass. The patient was uneventful after mitral valve plasty surgery in 2 years’ follow-up.

Conclusion

We diagnosed a large MVA of posterior leaflet without endocarditis by RT-3D-TEE here. Large MVAs did not collapse in diastole, but the jet flow from left ventricle into the aneurysm can help us distinguish MVA from mass. Besides, RT-3D-TEE was more reliable to diagnose and assess MVA than 2-dimentional transthoracic echocardiography.
Benzothiazole and benzoxazole promoted cleavage of Ru-C(aryl) bond in a four-membered ortho-metalated Ruthenium(II) organometallics
Authors: Chowdhury Jagannath , Ghosh Mrinal Kanti , Garu Purnananda , Chattopadhyay Swarup 
Published: November 2022

Abstract
The four-membered ruthenium(II) organometallics Ru(η2-RL)(PPh3)2(CO)(Cl) (1) where η2-RL = C6H2O-2-CHNHC6H4R(p)-3-Me-5 and R = CH3 reacts with 2-(2-hydroxyphenyl)benzothiazole (Hhpbt) and 2-(2-hydroxyphenyl)benzoxazole (Hhpbo) in refluxing ethanol to afford Ru(PPh3)2(CO)(hpbt)Cl (2) and Ru(PPh3)2(CO)(hpbo)Cl (3) respectively in excellent yield. In the course of these reactions the Ru-C(aryl) bond in 1 is cleaved and the RL ligand is no longer coordinated with the metal center in the products. The spectral (UV-vis, IR, 1H NMR) and electrochemical data of the complexes are reported. The identity of complex 2 has been established by single-crystal X–ray structure determination. The electronic structure and the absorption spectra of the complexes are scrutinized by DFT and TD-DFT analyses.
Serum CA19-9 as a predictor of incident metabolic syndrome in obese middle-aged and older men: A 9-year cohort study
Authors: Chang Pi-Kai , Hu Je-Ming , Wu Li-Wei 
Published: November 2022

Abstract
The status of carbohydrate antigen 19 − 9 (CA19-9) in metabolic syndrome (MetS) is unknown. From 2007 to 2015, 1,750 participants were retrospectively reviewed; health checkup data were obtained. Participants were divided into three groups based on CA19-9 levels. Body mass index (BMI), waist circumference (WC), and blood pressure were determined. Blood samples were collected after fasting for > 8 hours, to measure biochemical parameters and tumor markers. Cox regression analysis showed that, after adjusting for covariates, the highest CA19-9 tertile was associated with an increased risk of incident MetS (P = 0.002), high systolic blood pressure (≥ 130 mmHg; P < 0.001), high WC (≥ 90 cm; P < 0.001), and high fasting plasma glucose (≥ 100 mg/dL; P = 0.001), low high-density lipoprotein (≤ 50 mg/dL; P = 0.001), and high triglyceride (≥ 150 mg/dL; P = 0.001) levels. Subgroup analysis showed that individuals in the highest CA19-9 tertile who were obese (BMI ≥ 24 kg/m2; P = 0.002), male (P = 0.001), and ≥ 50 years of age (P = 0.002) were at increased risk of incident MetS. Our results revealed a positive correlation between CA19-9 levels and MetS in obese middle-aged and older men.
Discovery biomarker to optimize obeticholic acid treatment for non-alcoholic fatty liver disease
Authors: Lee Seung Min , Jun Dae Won , Yoon Eileen Laurel , Oh Ju Hee , Roh Yoon Jin , Lee Eun Jeoung , Shin Ji-Hee , Nam Young-Do , Kim Hyun Sung 
Published: November 2022

Abstract
Background & Aims: Though obeticholic acid (OCA) is a promising drug for non-alcoholic fatty liver disease (NAFLD), the response rate of OCA is limited. This study aimed to develop a biomarker to optimize OCA treatment for NAFLD.
Methods: C57BL/6N mice males were fed on a western diet for 24 weeks. Pre-study liver biopsy performed at 12 weeks, and stratified according to disease severity. Next, the mice were administered with OCA (5 mg/kg/day) or vehicle for additional eight weeks. Hepatic transcriptome, metabolome and intestinal microbiome analyses compared according to OCA treatment responder and non-responder using pre-study and end of study samples. LX-2 cells transfected with short-interfering RNA against CYP7B1(siCYP7B1) and/or treated with OCA to evaluate the role of CYP7B1 in NAFLD.
Results: Resolution rate of steatohepatitis in the OCA and vehicle groups were 36.8% and 0%, respectively. The hepatic transcriptome and bile acid metabolite profile analyses revealed that the alternative bile acid synthesis pathway (Cyp7b1 and muricholic acid) in the OCA-responder group were upregulated compared with those in the OCA-non-responder group. Intestinal microbiome analysis also revealed that the abundances of Bacteroidaceae, Parabacteroides, and Bacteroides, which were positively correlated with the alternative bile acid synthesis pathway, were higher in the OCA-responder group than in the non-responder group. Pre-study hepatic mRNA levels of Cyp8b1 (classic pathway) were downregulated in the OCA-responder group. The OCA response rate increased up to 80% in cases with a hepatic Cyp7b1/Cyp8b1 ratio ≥ 5.0. CYP7B1 expression was regulated by glucose concentration, and anti-fibrotic effect of OCA showed CYP7B1 dependent manner.
Conclusion: The upregulated alternative bile acid synthesis pathway or high hepatic CYP7B1 can be a potential biomarker for predicting OCA response.
Diurnal rhythm in cortical chloride homeostasis underpins functional changes in visual cortex excitability
Authors: Pracucci Enrico , Graham Robert , Alberio Laura , Nardi Gabriele , Cozzolino Olga , Pasquini Giacomo , Pillai Vinoshene , Saieva Luciano , Walsh Darren , Landi Silvia , Zhang Jinwei , Trevelyan Andrew , Ratto Gian Michele 
Published: November 2022

Abstract
Fast synaptic inhibition is a primary determinant of cortical activity patterns, and it is mediated predominantly by ionotropic, chloride-conducting receptors. Consequently, modulation of chloride homeostasis is ideally placed to regulate activity. We therefore investigated the stability of steady-state [Cl-]i in adult mice neocortex, using in vivo two photon imaging. We found a two-fold increase in baseline [Cl-]i in layer 2/3 pyramidal neurons, from day to night, with marked effects upon both physiological cortical processing and seizure susceptibility. Importantly, the night-time activity can be converted to day-time patterns by local inhibition of NKCC1. Consistent with these findings, the surface expression and phosphorylation states of the cation-chloride cotransporters, NKCC1 and KCC2, are diurnally modulated, with reduced chloride extrusion capacity at night. These data indicate that [Cl-]i modulation is likely to be an important determinant of variable cortical excitability, through the day.
The Glasgow prognostic score predicts survival in patients with advanced non-small cell lung cancer harboring sensitive EGFR mutations who are treated with tyrosine kinase inhibitors
Authors: Akazawa Yuki , Igawa Satoshi , Yamada Kaori , Yamamoto Hiroki , Yagami Yuri , Kaizuka Nobuki , Manaka Hiroya , Kasajima Masashi , Nakahara Yoshiro , Sato Takashi , Mitsufuji Hisashi , Yokoba Masanori , Kubota Masaru , Sasaki Jiichiro , Naoki Katsuhiko 
Published: November 2022

Abstract
Background: Epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) are the standard first-line treatment for advanced non-small cell lung cancer (NSCLC) with sensitive EGFR mutations. The Glasgow prognostic score (GPS) is an inflammation-assessing score based on C-reactive protein and albumin concentrations. Information regarding the association between the GPS and EGFR-TKI treatment effectiveness is limited; hence, we investigated whether the GPS can predict the response of NSCLC to EGFR-TKIs.
Methods: We evaluated 340 patients with NSCLC harboring sensitive EGFRmutations who received EGFR-TKI monotherapy between March 2009 and July 2021. The Kaplan–Meier method and Cox proportional hazards models were used to assess progression-free survival (PFS) and overall survival (OS).
Results: After a median follow-up of 26.6 months, patients with a GPS of 0, 1, and 2 had PFS of 15.7, 10.0, and 6.3 months, respectively, and OS of 40.1, 25.8, and 14.4 months, respectively; patients with a GPS of 0 had significantly better PFS and OS than those with a GPS of 1 (P=0.03, P=0.001, respectively) or 2 (P<0.001, P<0.001, respectively). Multivariate analysis identified poor performance status, stage IV at diagnosis, type of EGFR-TKI (gefitinib/erlotinib vs. afatinib), and GPS=2 as predictors of a short PFS. Meanwhile, poor performance status, gefitinib/erlotinib administration, and GPS=2 were predictive of a short OS.
Conclusions: The GPS predicted the survival of NSCLC patients harboring sensitive EGFRmutations who were undergoing EGFR-TKI treatment. The GPS might be ideal for routine use in clinical practice, given that it is an easily calculated parameter.