bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potassium Application Enhanced Plant Growth, Mineral Composition, Proximate and Phytochemical Content in Trachyandra divaricata Kunth (Sandkool)
Authors: Bulawa Bakholise , Sogoni Avela , Jimoh Muhali Olaide , Laubscher Charles Petrus 
Published: November 2022

Wild leafy vegetables are commonly included in the diet of people in rural homesteads. Among various wild edible vegetables in South Africa, Trachyandra divaricata (Sandkool) is one of the most abundant but underutilized due to the dearth of literature on its cultivation and nutritional value. In the present study, the effect of potassium application and pruning on growth dynamics, mineral composition, and proximate and phytochemical content in T. divaricata were evaluated. Treatments consisted of three potassium concentrations (0.0072, 0.0144, and 0.0216 M) supplemented in the form of potassium sulphate (K2SO4) with four pruning levels (unpruned, 5, 10, and 15 cm) applied in each treatment. The potassium doses were added to the nutrient solution, while the control treatment was sustained and irrigated with nutrient solution only. The results revealed a significant increase in flower bud yield, height, total dry and wet weight of shoots and roots, as well as ash and neutral detergent fibre in plants irrigated with 0.0072 M of K2SO4 without pruning. Conversely, chlorophyll content and Ca were comparable among treatments, while the highest yield of Na, P, N, and Zn was recorded in treatment 100 mL of K2SO4 with 10 cm pruning. Likewise, the highest antioxidant value (Polyphenols, Flavonol and DPPH) was obtained from plants irrigated with 0.0072 M of K2SO4 with 10 cm pruning. Based on these findings, T. divaricata is a promising leafy vegetable as a minimum dose (0.0072 M) of K with moderate pruning optimised its productivity in terms of growth, biomass parameters, nutritional content, and antioxidant potential. Due to its rich nutritional value, the plant should be domesticated and studied further for its potential nutraceutical benefits.
Modelo para el seguimiento y control de la deserción en la población universitaria
Authors: Hurtado-Tobón Luís Hernando , García-González María Dolly , Galvis-Soto Diana Milena , Méndez-Parra Rosa María 
Published: November 2022

El abandono de los estudios universitarios, por parte de los estudiantes sin obtener el título, representa además de la frustración para el individuo, su familia y su comunidad, una pérdida de recursos para el Estado y se puede mirar como una respuesta a situaciones de tipo individual, sociocultural, académico, institucional y económico de esa población joven.
Cuando se enfrenta la tarea de responder al problema de la deserción de los estudiantes, hay que puntualizar las especificidades de la población según su entorno, diagnosticar la situación específica de la institución y motivar a los estudiantes a culminar la formación profesional, lo que induce a la construcción de un modelo que reúna la acción institucional para controlarla, integrando varias dependencias de la estructura universitaria y manteniendo una estrecha relación con la calidad de la Educación en general; se trata de un asunto que no se resuelve consultando periódicamente cifras a través de un software especializado y de alto costo, sino de ejecutar acciones dirigidas a los aspirantes y a los estudiantes activos, con miras a lograr la permanencia hasta graduarse. El modelo para controlar la deserción en la Universidad del Quindío muestra un proceso constructivo y aplicado en una ventana de observación de 10 años, cuyos resultados más visibles son la disminución en la Deserción por Período y la determinación de las carreras de mayor aceptación entre los bachilleres aspirantes según sus habilidades.
Non-pharmaceutical primary care interventions to improve mental health in deprived populations: a systematic review
Authors: Tanner Louise M , Wildman Josephine M , Stoniute Akvile , Still Madeleine , Bernard Kate , Green Rhiannon , Eastaugh Claire H , Thomson Katie H , Sowden Sarah 
Published: November 2022

BackgroundCommon mental health disorders are especially prevalent among people from socioeconomically disadvantaged backgrounds. Non-pharmaceutical primary care interventions, such as social prescribing and collaborative care, provide alternatives to pharmaceutical treatments for common mental health disorders, but little is known about the impact of these interventions for patients who are socioeconomically disadvantaged.AimTo synthesise evidence for the effects of non-pharmaceutical primary care interventions on common mental health disorders and associated socioeconomic inequalities.Design and settingSystematic review of quantitative primary studies published in English and undertaken in high-income countries.MethodSix bibliographic databases were searched and additional grey literature sources screened. Data were extracted onto a standardised proforma and quality assessed using the Effective Public Health Practice Project tool. Data were synthesised narratively and effect direction plots were produced for each outcome.ResultsThirteen studies were included. Social-prescribing interventions were evaluated in 10 studies, collaborative care in two studies, and a new model of care in one study. Positive results (based on effect direction) were reported for the impact of the interventions on wellbeing in groups that were socioeconomically deprived. Inconsistent (mainly positive) results were reported for anxiety and depression. One study reported that people from the group with least deprivation, compared with the group with greatest deprivation, benefitted most from these interventions. Overall, study quality was weak.ConclusionTargeting non-pharmaceutical primary care interventions at areas of socioeconomic deprivation may help to reduce inequalities in mental health outcomes. However, only tentative conclusions can be drawn from the evidence in this review and more-robust research is required.
impacto da ratio studiorum nas aldeias do sertão
Authors: Carneiro Santana Rejane Cristine , Portella Monteiro de Souza Emília Helena 
Published: November 2022

O presente artigo ressalta a relevância da Ratio Studiorum à formação dos jesuítas e o seu desempenho com a catequese, nas missões dos “caminhos de dentro”, no período seiscentista, na colônia brasileira. Assim, propõe-se a um estudo de cunho bibliográfico e documental para compreender como a formação humanística da Ratio Studiorum contribuiu para o relacionamento e a atuação dos inacianos em sua missão, na América portuguesa, mais propriamente, nos sertões da capitania da Bahia. Também, toma-se o jesuíta Pe. Vicenzo Mamiani, que teve missão junto aos Kiriri e produziu um catecismo, Catecismo da Doutrina Christãa na Lingua Brasilica da Nação Kiriri (MAMIANI, 1698), e uma gramática, Arte de Grammatica da Lingua Brasilica da Naçam Karirí (MAMIANI, 1699), os quais serão objeto de análise. A pesquisa se situa no âmbito da historiografia linguística e da linguística histórica sócio-histórica e embasa-se nos estudos de Mattos e Silva (1993, 2006), Pompa (2003), Leite (2000), Cardim (1997), Sousa (1971), Miranda (2007), Rodrigues (1994), dentre outros, que trazem uma abordagem dos contatos entre jesuítas e indígenas, e questões sócio-históricas, no período da colonização brasileira. Como resultado, apresentam-se ações da catequese nas aldeias do semiárido baiano, que firmam a arte de doutrinar em língua Kiriri, como também, um levantamento da produção escrita referente à língua Kiriri, o catecismo e a gramática, produzidos por Mamiani, que evidenciam a sua formação humanística do Ratio Studiorum.
Pesquisar a educação infantil e o espaço com Fernand Deligny
Authors: Tebet Gabriela Guarnieri de Campos , De Oliveira Junior Wenceslao , Salles Isabela Ruivo 
Published: November 2022

Esse texto apresenta um diálogo entre Gabriela Tebet e Wenceslao Oliveira Jr, a partir do interesse comum de ambos no trabalho de Fernand Deligny que atravessa suas pesquisas que visam pensar os bebês a partir da cartografia ou pensar o espaço-escola da educação infantil a partir do cinema. É realizada uma breve apresentação de Deligny e seu trabalho aos leitores com destaque para as ideias de adaptação/ inadaptação; rede, território, linhas costumeiras e de errância bem como para o trabalho de cartografias desenvolvidos por Deligny. São exploradas de modo especial as possibilidades das contribuições de Deligny para o campo da educação infantil, da infância e dos bebês em especial.
Development of an Assessment Scale for the Risk of Falling in Pregnant Women
Authors: Koç Emine , Şahin Nevin Hotun 
Published: November 2022

ABSTRACT

Introduction
In pregnant women, the rate of falling is similar to that of women older than 70 years. According to the literature review, there is no risk of falling assessment tool that is specific to pregnancy. The aim of the study was to develop a risk of falling assessment scale for pregnant women.

Methods
This is a methodological study. The study's population consisted of pregnant women who sought follow-up care at a state hospital's maternity ward between November 2016 and November 2017. The study sample included 630 pregnant women who met the inclusion criteria and volunteered for the study. The Pregnant Women Information Form and Assessment Scale for Risk of Falling in Pregnant Women were used as data collection tools.

Results
During the scale development process, an item pool draft of 63 questions was developed and submitted to 10 experts for feedback. The findings of the content validity analysis revealed that the average of the items was 0.95, validity was good, and the number of items on the scale was reduced to 42 according to the experts' suggestions. The Cronbach α coefficient of the scale was found to be 0.604 (moderately reliable). It was discovered that the CART and QUEST algorithms on the scale were successful models for estimating the status of falls in pregnant women.

Conclusion
A 42-item assessment scale for the risk of falling in pregnant women was developed, and it was determined that the scale was a valid and reliable tool.
Challenges in Data Collection for Research During and After the Pandemic
Authors: Osman Malina 
Published: November 2022

The scope of data collection pertaining to particular outbreaks as well as general pandemics needs to be addressed accurately. All stakeholders in the crisis management need to be provided with precise information fairly and attentively. The urgency for the crisis solution through strategic innovations and well-coordinated services necessitates data analysis to be done rigorously in timely manners. It is to be subsequently shared with all the relevant agencies with the goal of benefiting the overall society.
Accordingly, we need to develop coordination that are built upon competent human resources, comprehensive infrastructure, reasonable budget allocation, close cooperation of all agencies as well as highly patriotic policy makers. In our situation, even potential challenges to utilise the data had been pre-identified before the pandemic but the situation has been and will be more demanding during and after this pandemic era.
A focus group involving researchers from multi sectoral agencies during post-Omicron wave have recently revealed complex issues which had never been discussed openly. They include dismal response from the stakeholders as they could not grasp any immediate benefits of any study, wrong perception that the research is futile and time consuming, lack of cooperation from the ‘gate keepers’ of those who hold the authoritative section, poor data coordination on sharing between agencies i.e. MOH and MOHE on several issues, insufficient regulatory commitment on research policies, tendencies for unnecessary red tapes, and unhealthy rivalry between MOH and MOHE on overlapping research interests.
This pandemic experience has also reared the backward culture of standing pride based on institutional stratification. The competition to outnumber others by favouring certain research brands of certain institutions over others should be put aside. The determination to resolve the uncertainties of the epidemic in the future must be executed with full responsibility and mutual respects.
The end of these endemic transition era is still uncertain, but we can’t afford to wait much longer to put everything in perspective. While various technologies in data management continue to evolve, the turning point to achieve the ideal mark/goal of data availability is inevitably now.
Evaluation of Bismuth Oxide Nanoparticles (BiONPs) as a Safe Radiobiological Enhancer for Breast Cancer Radiotherapy
Authors: Mohd Zainudin Nur Hamizah , Talik Sisin Noor Nabilah , Ab Razak Khairunisak , Abdullah Reduan , Rahman Wan Nordiana 
Published: November 2022

Cancer incidence has been increasing over the years and it is the second leading cause of death globally [1]. The therapeutic strategies in killing the cancerous tissue while keeping the normal healthy tissue uninterrupted can be further improved by introducing nanoparticles (NPs) as radiosensitizers in radiotherapy. In pre-clinical research, a few nanoparticle elements had shown the potential to be radiosensitizers, such as gold, superparamagnetic iron oxide, platinum, and bismuth nanoparticles. Bismuth oxide nanoparticles (BiONPs) have been investigated as a potential radiosensitizer in radiotherapy due to their least toxic and biocompatibility properties. In addition, due to the presence of metallic nanoparticles in cells and their environment, more DNA damage will be introduced and thus enhance the radiation treatment efficacy.

This research project was conducted to evaluate the potential of BiONPs to increase the radiation treatment in MCF-7 breast cancer cells and their side effects on the non-targeted breast cancer cells. BiONPs were synthesized through the hydrothermal method, as established in the previous study [2]. The treated and control cells in flasks were irradiated with radiation doses between 0 to 12 Gy at a constant dose rate of 300 cGy/minutes in the beam field size of 10 cm x 10 cm for a 6 MV photon beam, delivered with linear accelerator Primus model (Siemen Healthcare, USA). The irradiated cell-conditioned medium (ICCM) was collected from the targeted cells and transferred into the non-targeted cells. The cell viability assay was employed to evaluate the effect of BiONPs on directly irradiated and non-irradiated cells.

In our prior work, the BiONPs had shown to boost radiosensitisation effects in treated cells compared to control cells, with a sensitisation enhancement ratio (SER) of 1.38 [3], as shown in Figure 1. The increased radiosensitisation effects might be attributed to the characteristics of BiONPs with a high effective atomic number (Z=83), which promotes radiation interaction, mainly radiation absorption and scattering. Meanwhile, the present study demonstrated that the non-irradiated MCF-7 cells could maintain their cell viability for more than 80% after 48 h incubation with ICCM treated with BiONPs at 2, 6, and 12 Gy. Furthermore, a comparable trend in cell viability was observed in non-irradiated MCF-7 cells for treated and control groups (p<0.05), as illustrated in Figure 2. This finding indicated that BiONPs are not harmful and do not contribute to side effects in non-targeted cells.
The present works have provided evidence that using BiONPs as radiosensitizers in radiotherapy is safe and does not significantly introduce side effects in the non-targeted cells. These data proved the impacts of BiONPs as a potential tool for a safe radiobiological enhancer that will benefit future radiation therapy strategies in cancer management.
Development of a sensitive, specific and cost-effective T-ARMS PCR assay for the genotyping of R132H of IDH1 gene in glioma patients
Authors: Pua Jing Yit , Lee Ang , Khor Vienna Zi Wei , Pushpanathan Thanaseelan , Mohamed  Yusoff Abdul Aziz , Idris Zamzuri , Patar Azim 
Published: November 2022

The discovery of isocitrate dehydrogenase isoform 1 (IDH1) mutation as a key molecular marker has resulted in a change in glial tumour classification [1]. IDH1 mutations are commonly in gliomas, particularly in low-grade gliomas and secondary glioblastoma [2]. IDH1 p.R132H (c.395G>A) accounted for more than 90% of the mutation in IDH1/2 mutation and had a significant association with clinical outcomes. IDH1/2 mutations cause gain-of-function resulting in the formation of an oncometabolite, R-2-hydroxyglutarate instead of α-ketoglutarate implying a disruption of oxidative decarboxylation of Kreb’s cycle and other cellular mechanisms [3,4,5]. Immunohistochemistry (IHC) and Sanger sequencing is the most common approach used in molecular diagnostics. Nevertheless, both IHC and Sanger sequencing methods have shortcomings. IHC is prone to miss out on the mutation if the tumour samples are of poor quality, and it has also been reported to have low sensitivity when compared to sequencing-based techniques [6,7].

This study aimed to develop a sensitive, specific and cost-effective assay for genotyping IDH1 p.R132H mutations in glioma patients in order to shorten the time required for confirmatory diagnosis to be made. The tetra primer amplification refractory mutation system polymerase chain reaction (T-ARMS PCR) was used in this study to develop and validate the clinical applicability of the assay. A total of 61 glial specimens were collected and genomic DNA was isolated from all of them. All the samples were subjected to endpoint PCR and Sanger sequencing for mutation detection. T-ARMS PCR was developed and optimized prior to the screening of all the samples, and comparative mutation analysis was carried out.
Overall, IDH1 p.R132H mutation was found to be 45.90% (n=28/61) prevalent and was found to be significantly associated with gender, tumour subtypes and grading, and location of the lesions (p=<0.05). With an F1 score of 0.966, we reported T-ARMS PCR with sensitivity, specificity and accuracy of 100% (95% CI: 87.94-100.00%), 93.94% (95% CI: 80.39-98.92%) and 96.72%, respectively. To compare with published studies, a meta-analysis is T-ARMS PCR detected one case with IDH1 p.R132C (c.394C>T), howbeit the case could have a double mutation of p.R132C (c.394C>T) and p.R132H (c.395G>A) or single mutation of p.R132Y (c.394_395CG>TA). However, this study was able to detect IDH1 p.R132G (c.394C>G) mutation via PCR by Sanger sequencing whereas T-ARMS PCR excluded the mutation, suggesting the assay is very specific to IDH1 p.R132HH (c.395G>A) only.
Hereby, the performance of the T-ARMS PCR assay sheds a light that can be adapted for preoperative or intraoperative diagnosis.
Association of Predisposing Haplotype Snp-Markers of HBB Gene in Thalassaemia
Authors: Erwan Mohamad Naiem Firdaus , Wan Taib Wan Rohani 
Published: November 2022

Most of the beta thalassaemia cases are heterogeneous at the molecular level. The genetic variation arises due to the mutation of a single beta-globin gene (HBB) on chromosome 11p15.5. Eventhough, the basis and natural history of this disorder are still not well understood and the clinical presentation in certain patients was not always correlated well with the single gene diagnosis [1].

The study aimed to evaluate the predisposing effect of HBB polymorphisms and their haplotypes in conferring beta-thalassaemia in Malaysia using a bioinformatics tool. Archived genotype data for five tagging SNPs of HBB gene assigned as c.9T/C, IVS2-16G/C, IVS2-74T/G, 3'UTR +314G/A and 3'UTR +316A/C across thalassaemia patient group (n=433) and normal control group (n=794) controls were retrieved from the year 2011 to 2016 at Institute for Medical Research (IMR). The genotype data were analysed for individual SNP-based association analysis and haplotype-based association analysis using SHEsis online software.

The study in all races in Malaysia showed significant association in four SNPs of the HBB gene (c.9T/C, P-value = 0.014; IVS2-16G/C, P-value = 0.017; 3'UTR+314G/A, P value = 2.08x108; 3'UTR+316A/C, P-value = 0.021). However, IVS2-74T/G did not show significant association with a P-value of 0.763 (Table 1). We further revealed two susceptible haplotypes in all races (haplotype TGTAA, P-value = 1.68x10-7, OR = 2.382 [1.707~3.325]; haplotype CCTGC, P-value = 0.003, OR = 1.300 [1.089~1.551]). Meanwhile, one protective haplotype observed was haplotype TGTGA (P-value = 2.99x10-7, OR = 0.625 [0.522~0.749]) (Table 2).
In conclusion, this study suggests the potential susceptible and protective haplotypes of the HBB gene in predisposing to beta-thalassaemia in Malaysia
Haematological and Molecular Characterisation of Haemoglobin E with Deletional Alpha-Thalassaemia among Kelantan Subjects
Authors: Muhamad Yasin Nur Hidayah , Mat-Ghani Siti Nor Assyuhada , Zulkafili Zefarina , Mohd Noor Noor Haslina , Hassan Mohd Nazri , Wan Abdul Rahman Wan Suriana , Bahar Rosnah , Mohd  Yusoff Shafini , Abdullah Marne , Iberahim Salfarina , Ramli Majdan , Ramli Marini 
Published: November 2022

Heterozygous haemoglobin E (Hb E) with deletional alpha (α)-thalassaemia is commonly seen in Southeast Asia including Kelantan, a Northeastern state of Malaysia. Studies in Malaysia showed that Hb E is the commonest among Malay Kelantan, Penang and Senoi group of Orang Asli [1-5].

This study aimed to compare the haematological parameters (Hb, RBC, MCV, MCH, RDW and Hb E) among the heterozygous Hb E with deletional α-thalassaemia subjects. This study also intended to ascertain a cut-off value for heterozygous Hb E with deletional α-thalassaemia using automated capillary electrophoresis method.

A cross-sectional study was conducted involving secondary data collection of 219 samples of heterozygous Hb E with possible α-thalassaemia from all districts of Kelantan. Full blood count (FBC) was analysed using Sysmex XN 3000 automated blood cell analyser (Kobe, Japan). Hemoglobin (Hb) analysis was performed using automated CE system (Capillarys2; Sebia, France). Molecular characterisation was performed using multiplex gap-PCR and ARMS-PCR to detect both deletional and non-deletional α-thalassaemia, respectively. Qualitative data were expressed as frequency and percentage while quantitative data were expressed as mean, ± SEM and median. One-way ANOVA was used to test for mean Hb level, RBC count, MCV level, MCH level, and RDW level differences among six groups of heterozygous Hb E with concurrent deletional alpha thalassaemia in this study. The ROC curve was used to determine the cut-off point for Hb E level.

Eighty-nine samples of heterozygous Hb E were confirmed to have concurrent deletional α-thalassaemia. Heterozygous Hb E with heterozygous α3.7 gene deletion was the most common deletional α-thalassaemia observed in 56 samples (62.9%) and followed by heterozygous SEA gene deletion which were detected in 21 samples (23.6%). The haematological parameters showed there was a significant difference between groups. The cut-off point for Hb E level for predicting heterozygous Hb E with deletional α-thalassaemia from CE method was 24.7% (Table 1). The diagnostic performance for the cut-off point for Hb E level showed sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 100% for all.
This cut-off value can be used in assisting the selection of heterozygous Hb E individual to proceed with DNA analysis for α-thalassaemia.