bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of ocean acidification on the growth, response and hydrocarbon degradation of coccolithophore-bacterial communities exposed to crude oil
Authors: Fahmi Afiq Mohd , Summers Stephen , Jones Martin , Bowler Bernard , Hennige Sebastian , Gutierrez Tony 
Published: September 2022

Abstract
Hydrocarbon-degrading bacteria, which can be found living with eukaryotic phytoplankton, play a pivotal role in the fate of oil spillage to the marine environment. Considering the susceptibility of calcium carbonate-bearing phytoplankton under future ocean acidification conditions, and their oil-degrading communities to oil exposure under such conditions, here we investigated the response of non-axenic E. huxleyi to crude oil under ambient versus elevated CO2 concentrations. Under elevated CO2 conditions, exposure to crude oil resulted in the immediate decline of E. huxleyi, with concomitant shifts in the relative abundance of Alphaproteobacteria and Gammaproteobacteria. Survival of E. huxleyi under ambient conditions following oil enrichment was likely facilitated by enrichment of oil-degraders Methylobacterium and Sphingomonas, while the increase in relative abundance of Marinobacter and unclassified Gammaproteobacteria may have increased competitive pressure with E. huxleyi for micronutrient acquisition. Biodegradation of the oil was not affected by elevated CO2 despite a shift in relative abundance of key hydrocarbon degraders. While ocean acidification does not appear to affect microbial degradation of crude oil, elevated mortality responses of E. huxleyi and shifts in the bacterial community illustrates the complexity of microalgal-bacterial interactions and highlights the need to factor these into future ecosystem recovery projections.
The use of surnames to impute missing ethnicity data in the South African National Cancer Registry database
Authors: Chen Wenlong Carl , Kellett Patricia , Greyling Mike , Singh Elvira , Sengayi-Muchengeti Mazvita 
Published: September 2022

Abstract
The National Cancer Registry (NCR) of South Africa (SA) calculates cancer incidence rates based on full pathology reports from South African private and public health care laboratories and presents the cancer incidence data by ethnic groups. The sensitivity of collecting ethnicity data in post-apartheid South Africa by reporting sources has resulted in large proportions of cancer cases being reported without population group/ethnicity information. The absence of ethnicity data is a significant challenge to cancer incidence reporting. An imputation method was developed to impute the missing ethnicities by using surnames with known patient-reported ethnicities. A hold-out test done by masking the ethnicities of 50% (n = 332232) of the NCR dataset with known ethnicities, from 1986 to 2014, was used to evaluate this imputation method. The masked ethnicities were imputed and then compared to the patient-reported ethnicities. 94.31% of ethnicities were correctly classified using this imputation method. Sensitivities and specificities were calculated per ethnicity group (Asian, Black, Coloured, White). The imputation method performed well for the Asian, Black and White ethnic groups, but performed poorly for the Coloured ethnic group. The strong relationship between surnames and ethnic groups, as evidenced by the results, mitigates the significant concern of whether surname itself is predictive of ethnicity. Despite the increasing proportion of missing data over the years, the percentage of correctly classified individuals remains high across the test dataset. The strength of this imputation methodology is demonstrated in this study, however, with the large disparities across the private and public healthcare sectors in SA, all cancer cases should be reported with complete information, from all sources, for accurate cancer incidence reporting without the need for having to impute for missing data. There are still challenges around collecting sensitive data such as ethnicities in a SA that warrant further discussions.
Implementation analysis of municipal solid waste management in Dinanagar city of Punjab, India
Authors: KAUR MANBIR , Yadav Rakesh 
Published: September 2022

Abstract
Unscientific waste management is increasingly becoming a major reason for environmental issues in Indian cities. Unlike previous municipal solid waste management in cities of Punjab(India ), this study analyzes the implementation of solid waste management. Also, examine the factors responsible for the dysfunction of the municipal corporation of Dinanagar(MCD) city of Punjab(India). To fulfill the research objectives, primary and secondary data are collected from various sources for qualitative and quantitative analysis. However, some drawbacks and flaws were found in the existing practices of municipal solid waste management. Internal consistency and validity are measured using Cronbach’s alpha. The importance-performance analysis and strengths, weaknesses, opportunities, and threats analysis are performed to conclude the present scenario of MCD. This study eventually concluded with some suggestions to waste management authorities and researchers for contributions to the improvement of the current system.
ZNNT1/osteopontin/S100A9 feedback loop promote hepatocellular carcinoma progression via mediating crosstalk between hepatocellular carcinoma cells and macrophages
Authors: Wei Huamei , Li Wenchuan , Yang Meng , Fang Quan , Nian Jiahui , Huang Youguan , Wei Qing , Huang Zihua , Liu Guoman , Xu Zuoming , Hu Anbin , Pu Jian 
Published: September 2022

Abstract
Background
Macrophages are the major components of tumour microenvironment, which play critical roles in tumour development. Long noncoding RNAs (lncRNAs) also contribute to tumour progression. However, the potential roles of lncRNAs in modulating the interaction between cancer cells and macrophages in hepatocellular carcinoma (HCC) are poorly understood.
Methods
The expression of lncRNA ZNNT1 in tissues and cells was measured using qRT-PCR. The roles of ZNNT1 in HCC cells and macrophages were investigated using in vitro and in vivo assays. The molecular mechanisms of ZNNT1 were explored using qRT-PCR, RNA immunoprecipitation, RNA pull-down, chromatin immunoprecipitation, enzyme linked immunosorbent assay, and dual-luciferase reporter assays.
Results
ZNNT1 was identified as an HCC-related lncRNA, which was upregulated and associated with poor prognosis of HCC. ZNNT1 promoted HCC cellular growth, migration, and invasion, and suppressed apoptosis in vitro. ZNNT1 promoted HCC xenograft growth in vivo. Furthermore, ZNNT1 recruited and induced M2 polarization of macrophages. Mechanistically, ZNNT1 upregulated SPP1 expression and osteopontin (OPN) secretion via sponging miR-181a/b/c/d-5p and miR-33a/b-5p. Functional rescue assays identified OPN as the mediator of the oncogenic roles of ZNNT1 in HCC cells and also the effects of ZNNT1 on macrophages. M2 Macrophages-recruited by ZNNT1 enhanced malignant phenotypes of HCC cells, which was mediated by S100A9 secreted by M2 macrophages. Intriguing, S100A9 secreted by M2 macrophages also upregulated ZNNT1 expression in HCC cells via AGER/NF-κB signaling.
Conclusions
ZNNT1, OPN, and S100A9 formed a positive feedback loop, which promoted macrophages recruitment and M2 polarization, and enhanced malignant features of HCC cells. The ZNNT1/OPN/S100A9 feedback loop represents potential therapeutic target for HCC.
WITHDRAWN: Novel hydro-alcoholic sanitizer model with minimum alcohol content and fortified with eugenol and cinnamaldehyde as natural antimicrobial boosters
Authors: Shaaban Hamdy A. , Abdelhamid Samy M. , Edris Amr 
Published: September 2022

Abstract

Objectives
70% alcohol-based sanitizers are frequently used for decontamination and controlling infections caused by pathogenic microorganisms. In the current investigation the authors aimed at developing and evaluating novel diluted hydro-alcoholic sanitizer containing 70% water and less than 30% alcohol. That is due to the fact that excessive use of alcoholic sanitizers containing 70% alcohol leads to immergence of alcohol resistant strains of pathogenic bacteria. In addition, some other toxic and environmental issues arise from using such high amounts of alcohol in the common sanitizing formula. The developed formula in the current work was fortified with some natural anti-microbial boosters from aromatic plants like eugenol, cinnamaldehyde and their mixture to compensate for the low alcohol content.
Methods
The solubility of eugenol and cinnamaldehyde in 70% water-containing hydro-alcoholic solution was investigated using the Gibbs’ triangle phase diagram. The broth dilution assay was used to evaluate the growth inhibition of the developed formula against four pathogenic bacteria including L. monocytogenes, S. typhimurium, E. coli and P. aeruginosa.
Results
The phase diagram study indicated that 3% of each of the antimicrobial boosters (eugenol, cinnamaldehyde) can be incorporated homogenously into a diluted hydro-alcoholic sanitizer containing 70% water and only 27% alcohol. Antimicrobial evaluation of that formula at only half concentration of the antimicrobial boosters (1.5%) showed complete inhibition of the growth of the four tested pathogens, where the count after treatment was zero cfu/ml. This antimicrobial activity was similar to that of a standard alcoholic sanitizer that contains 70% alcohol.
Conclusions
The study represents an insight towards a new generation of sanitizers which are characterized by significantly minimum content of alcohol without compromising efficiency. That also represents a step toward lowering alcohol toxicity and reducing its vapors emission to the environment.
Taurine improves the growth performance, health status and liver histopathology of meagre (Argyrosomus regius) fed a reduced fish meal diet
Authors: Güroy Derya , Karadal Onur , Güroy Betül , Emre Yılmaz , Emre Nesrin , Eraslan Durali , Yalım Fatma Banu , Mantoğlu Serhan , Demir Abdullah 
Published: September 2022

Abstract
Research has focused on alternative plant materials or additives that can be used instead of fish meal due to limited stocks and increased feed prices, although it is important for carnivorous fish species. Taurine is a functional amino acid supplemented to low fish meal diets to improve the growth performance of fish. In this study, eight experimental diets with 0%, 0.5%, 1% and 2% ratios of taurine supplemented (T0, T0.5, T1 and T2) high (HFM) and low fish meal (LFM) diets were prepared (defined as HFMT0, HFMT0.5, HFMT1, HFMT2, LFMT0, LFMT0.5, LFMT1 and LFMT2). The experiment was carried out in twenty-four 400 L rectangular fiberglass tanks conducted in a marine water system, and fish (initial mean weight of 23.5 g) were stocked in each tank. The nutrition trial was carried out for 90 days with three replicates. Meagre fed with the HFMT1 diet had a higher final mean weight (FMW) and specific growth rate (SGR) than fish fed with HFMT0 and all LFM diets. Including taurine in all LFM diets also enhanced growth performance. The FMW and SGR of fish fed the LFMT0.5 diet were similar when compared to HFMT0 (P > 0.05) and increased than that of other LFM diets with taurine (P < 0.05). Meagre fed the LFM0.5 diet had a lower feed conversion rate (FCR) than those provided for all HFM diets, although there was no significant difference between treatments. Dietary taurine has decreased the hepatosomatic index (HSI), viscerosomatic index (VSI) and total ammonia-nitrogen excretion (TAN), irrespective of taurine and fish meal levels. Including dietary taurine at both fish meal levels enhance the number of red blood cells (RBC) and whole-body amino acids of meagre. The most remarkable conclusion of this study is the inclusion of taurine in low fish meal diets improved growth performance and profitability in meagre, and the level of 0.5% has been recommended.
Preoperative Incentive Spirometer to Prevent Postoperative Pulmonary Complications following Open Heart Surgeries: A Randomized Single Blinded Multi-Centric Clinical Trial
Authors: Noor Faleh Jadeel , AL-Fayyadh Sadeq 
Published: September 2022

Objectives: To determine the effect preoperative use of an Incentive Spirometer (IS) has on preventing postoperative pulmonary complications among patients undergoing open heart surgeries.
Methods: A prospective, randomized, controlled single-blinded parallel-group design. Data were collected from January 9th 2022 to April 1st 2022. A total of n = 64 eligible patients were equally randomized to either intervention or control group. Patients in the intervention group performed preoperative IS training for two days before the scheduled surgery. The primary endpoint was the incidence of in-hospital PPCs. The secondary endpoints were postoperative Peak Expiratory Flow Rate (PEFR), Intensive Care Unit (ICU) stay, Hospital Length of Stay (HLOS).
Results: Patients in the intervention group had a lower incidence of postoperative pulmonary complications compared to the control group (12.5% and 21.8%). There was a statistical significant difference between the two groups regarding postoperative PEFR, HLOS and intensive care unit stay.
Conclusions: Preoperative rehabilitation (involving incentive spirometer is related to a lower incidence of postoperative pulmonary complications for the group that received the intervention. Training with preoperative incentive spirometer resulted in a shortening of hospital length of stay and Intensive Care Unit (ICU) stay.
Trial registration: The study was registered in the Iranian Clinical Trial Registry, this at https://www.irct.ir/ reference number (IRCT20211224053503N1).
Integrating Alteryx Designer and Tableau Desktop into the AIS Course: An Analytics Mindset Model
Authors: Zhang Chuancai , Stone Dan N. 
Published: September 2022

ABSTRACT
This paper presents one approach to integrating data analytics (DA) and visualization content and software into the accounting information systems (AIS) course. The proposed AIS course model—which includes core and support components—covers many topics in the proposed 2024 CPA Evolution Model curriculum. The core components include an instructor-chosen AIS textbook and Alteryx Designer and Tableau Desktop software. The popularity, availability, ease of learning, and use of the Alteryx and Tableau software motivate choosing the software. The support components include weekly homework, a class project, professional certification exams, and a course survey. The course learning objectives facilitate (1) developing an analytics mindset, (2) cultivating an interest in accounting analytics and AIS, and (3) enhancing life-long self-directed learning in students. Instructors can implement the proposed course model in provided 15-, ten-, or four-week sessions. Evidence of efficacy suggests that the proposed course model meets its key learning objectives.
Profil Aberasi Kromosom Pasien Malformasi Kongenital Multipel Non-Sindromik
Authors: Aswin Yulia Ariani , Rachmadi Rizky Abi 
Published: September 2022

Malformasi kongenital multipel (MKM) non-sindromik merupakan penyakit neonatal dengan mortalitas tinggi, menurunkan kualitas hidup anak dan mahalnya biaya perawatan. Untuk mendiagnosis MKM-non-sindromik diperlukan analisis kromosom secara akurat dengan biaya terjangkau yaitu metode G-banding, namun detection rate belum diketahui. Penelitian bertujuan untuk mengetahui proporsi MKM non-sindromik yang dapat didiagnosis dengan metode G-banding. Penelitian ini menggunakan desain potong lintang dengan subjek semua pasien yang datang ke laboratorium sitogenetik Departemen Biologi FKUI pada Juli 2014 sampai dengan Desember 2017. Pasien dilakukan pemeriksaan fisis kemudian dibandingkan dengan basis data Online Mendelian Inheritance in Man (OMIM) untuk menyeleksi pasien MKM sindromik dan non-sindromik. Sebanyak 34 dari 83 subjek (41%) dapat ditegakkan diagnosis klinis menggunakan database fenotip. Sebanyak 49 pasien memenuhi kriteria penelitian kemudian dilakukan analisis kromosom dengan metode G-banding. Terdapat 17 (34,7%) subyek yang menunjukkan aberasi kromosom. Tiga fenotip tersering pada subjek adalah hambatan pertumbuhan, mikrosefali, dan penyakit jantung bawaan. Disimpulkan metode G-banding sebagai pemeriksaan skrining awal pada MKM berperan penting dalam mendiagnosis penyebab genetik.
Kata kunci: MKM non-sindromik, basis data fenotip, Indonesia, G-banding.

Chromosome Aberration Profiles in Patients with Non-Syndromic Multiple Congenital Malformation
Abstract
Non-syndromic multiple congenital malformations (MCM) remains a significant problem in Indonesia Chromosome Aberration Profiles in Patients with Non-Syndromic Multiple Congenital Malformation
as it causes high mortality and morbidity rate in neonates and infants as well as a significant financial burden for families in caring for patients with non-syndromic MCM. Genetics is the major cause of non-syndromic MCM, and more than 50% is caused by chromosomal abnormalities. Considering the condition in Indonesia, where most families have a low socioeconomic background and where there are limited molecular genetics laboratory facilities, this research aims to study the proportion of non-syndromic MCM that can be detected using G-banding and describe chromosome aberration profiles in non-syndromic MCM patients. The Online Mendelian Inheritance in Man (OMIM) was used to differentiate non-syndromic and syndromic MCM in 83 recruited MCM patients. Thirtyfour patients (41%) were diagnosed using a phenotype database based on clinical signs. The three most common phenotypes were growth failure, microcephaly, and congenital heart diseases. Forty-nine patients were classified with non-syndromic MCM after filtering with OMIM and underwent G-banding. Seventeen out of 49 non-syndromic MCM patients (34.7%) had chromosome aberration after G-banding. This indicates that G-banding remains an effective first-line screening tool for non-syndromic MCM in Indonesia.
Keywords: non-syndromic multiple congenital malformation, phenotype database, G-banding, Indonesia.
Strategi Komunikasi Eksternal Public Relations Pemerintah Kota Salatiga di Masa Pandemi Covid-19
Authors: Sulistiani Tarra Nur Azizah , Wijaya Lina Sinatra 
Published: September 2022

Salatiga merupakan salah satu kota di Jawa Tengah yang juga menujukkan kenaikan dalam jumlah kasus Covid-19. Oleh karena itu, Public Relations atau biasanya disebut juga Humas pemerintah Kota Salatiga merancang suatu strategi untuk mengurangi penyebaran Covid 19 di Salatiga dengan melaksanakan suatu strategi yaitu memberikan jarak bagi pedagang pasar pada pagi hari yang dilaksanakan mulai tanggal 27 April 2020. Pasar merupakan salah satu tujuan dari program humas, dikarenakan pasar merupakan salah satu sarang terjadinya keramaian masyarakat. Namun sayangnya, kasus positif Covid-19 di Salatiga belum menunjukkan penurunan. Penelitian ini bertujuan untuk mengetahui apa saja strategi komunikasi yang dilakukan oleh Public Relations Department Pemerintah Kota Salatiga di masa pandemic Covid-19 dan langkah-langkah strategi komunikasi yang dilakukan oleh Public Relations atau Humas Pemerintah Kota Salatiga. Penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan studi kasus. Teknik pengumpulan data berupa wawancara, observasi dan dokumen. Hasil penelitian ini menunjukan bahwa strategi komunikasi yang dilakukan oleh Public Relations Pemerintah Kota Salatiga adalah dengan menggunakan sarana media sosial, media massa, spanduk, baliho, videotron, siaran keliling, dan pusat informasi. Dalam menentukan sarana komunikasi yang akan digunakan tersebut, Public Relations Pemerintah kota Salatiga melaksanakan empat langkah strategi komunikasi yaitu: mengenal khalayak, menentukan topik/tema, menyusun narasi, dan akhirnya memilih media yang akan digunakan dalam jangka waktu tertentu.
Hubungan Bimbingan Guru Terhadap Kemandirian Anak Di Taman Kanak-Kanak Gugus Bougenville1 Kecamatan Koto Tangah Padang
Authors: Mardiani Tia , Zulminiati Zulminiati , Mahyuddin Nenny 
Published: September 2022

Tujuan penelitian ini adalah untuk mengetahui hubungan bimingan guru terhadap kemandirian anak di Taman Kanak-kanak Gugus Bougenville 1 Kecamatan Koto Tangah Padang. Kemandirian anak di Taman Kanak-kanak Gugus Bougenville 1 menunjukkan bahwa masih ada anak yang kurang percaya diri, bertanggung jawab, displin dalam kegiatan sekolah. Jenis penelitian ini adalah penelitian kuantitatif korelasional menggunakan teknik sampling total dengan jumlah populasi 26 orang guru. Berdasarkan analisis data, diperoleh koefisien korelasi antara pembiasaan dengan kemandirian anak sebesar 0,772 dan koefisien determinasinya sebesar 0,596. Hasilnya diperoleh bimbingan guru sebesar 59,6 % terhadap kemandirian anak signifikan pada taraf 5%. Dapat disimpulkan bahwa bimbingan guru mempunyai hubungan yang signifikan terhadap kemandirian anak di Taman Kanak-kanak Gugus Bougenville 1 Kecamatan Koto Tangah Padang sebesar 59,6.% dengan koefisien korelasi kuat
Penerapan Sentra Ibadah Di Taman Kanak-Kanak Islam Excellent Bukittinggi
Authors: Rahim Zhilla , Pransiska Rismareni , Ismet Syahrul 
Published: September 2022

Penelitian ini bertujuan untuk mendeskripsikan kegiatan pembelajaran sentra ibadah yang meliputi perencanaan, pelaksanaan dan evaluasi di Taman Kanak-Kanak Islam Excellent Bukittinggi. Penelitian ini menggunakan metode deskriptif dengen pendekatan kualitatif. Teki pengumpulan data yang digunakan terdiri dari observasi, wawancara dan dokumentasi. Teknik analisis Teknik analisis data dilakukan melalui reduksi data, penyajian data dan verifikasi data. Sedangkan teknik pengabsahan data menggunakan uji credibility, uji transferability, uji dependability, dan uji konfirmability. Hasil penelitian menunjukkan bahwa Penerapan sentra Ibadah di Taman Kanak-Kanak Islam Excellent Bukittinggi telah dilaksanakan dengan baik. Guru membuat perencanaan sebelum kegiatan dilakasanakan, kegiatan yang dilakukan disesuaikan dengan tujuan yang ingin dicapai menggunakan metode dan media yang sudah dirancang, serta evaluasi dilakukan pada setiap kegiatan