bluuebunny/crossref_metadata_2025_split huggingface dataset:
Firms’ innovation and university cooperation. New evidence from a survey of Italian firms
Authors: Bragoli Daniela , Cortelezzi Flavia , Rigon Massimiliano 
Published: December 2022

Abstract
In this article, we investigate whether the cooperation with universities may stimulate the innovative performance of Italian firms. We use a dataset merging information from two different surveys carried out by the Bank of Italy between 2007 and 2010. We derive our results using a two-stage procedure with the aim of ruling out spurious correlations due to the existence of omitted variables. Results show that the cooperation with universities does not affect the likelihood of firms introducing technological innovations. However, when we distinguish between pure technological innovation outcomes (only new products and/or productive processes) and joint innovation outcomes, which involve both organizational and technological changes, we find that only the latter is positively stimulated by the cooperation with universities. These findings are promising since, according to the innovation management literature, joint innovation activities are more successful in transferring new ideas and new business opportunities into market success.
Do children increase the likelihood of homeownership? Evidence from a sample with twins
Authors: Kim Seolah , Ryu Hanbyul 
Published: December 2022

Abstract
Homeownership status has a broad range of positive outcomes that accrue to both individuals and society. Although the determinants of homeownership have been studied in the past, there was little attention to the effect of family size. Using a twin birth as an exogenous change in the number of children, we examine the effect of increasing family size on the likelihood of owning a house. We found that having an additional child after the first birth increases the likelihood of homeownership approximately by 4.3%, but the impact faded out in subsequent births. In addition, family size is more likely to increase homeownership for less educated households and those who had first births at older ages.
The inferior frontal gyrus and familial risk for bipolar disorder
Authors: Qin Kun , Sweeney John A , DelBello Melissa P 
Published: December 2022

Abstract
Bipolar disorder (BD) is a familial disorder with high heritability. Genetic factors have been linked to the pathogenesis of BD. Relatives of probands with BD who are at familial risk can exhibit brain abnormalities prior to illness onset. Given its involvement in prefrontal cognitive control and in frontolimbic circuitry that regulates emotional reactivity, the inferior frontal gyrus (IFG) has been a focus of research in studies of BD-related pathology and BD-risk mechanism. In this review, we discuss multimodal neuroimaging findings of the IFG based on studies comparing at-risk relatives and low-risk controls. Review of these studies in at-risk cases suggests the presence of both risk and resilience markers related to the IFG. At-risk individuals exhibited larger gray matter volume and increased functional activities in IFG compared with low-risk controls, which might result from an adaptive brain compensation to support emotion regulation as an aspect of psychological resilience. Functional connectivity between IFG and downstream limbic or striatal areas was typically decreased in at-risk individuals relative to controls, which could contribute to risk-related problems of cognitive and emotional control. Large-scale and longitudinal investigations on at-risk individuals will further elucidate the role of IFG and other brain regions in relation to familial risk for BD, and together guide identification of at-risk individuals for primary prevention.
The contraction of the definition of beneficiary in Jersey—is it real or imagined?
Authors: Hooper Katie , Lewis Paul 
Published: December 2022

Abstract
Two recent decisions of the Royal Court of Jersey have focused attention on the nature of a beneficiary’s interest under a discretionary trust in Jersey. In Kea Investments Limited v Watson,1 the Court considered whether the interests of a discretionary beneficiary could be distrained upon pursuant to Article 10(11) of the Trusts (Jersey) Law 1984. In Patel & Ors v JTC Trust Company Limited,2 the Master of the Royal Court considered the separate question of whether the Royal Court had the power to impound the interest of a discretionary beneficiary pursuant to Article 46 of the Trusts (Jersey) Law 1984. These decisions provoke thought as to how we must now consider the nature of a discretionary beneficiary’s interest under a Jersey trust. While being separate and distinct decisions, they arguably share a common thread as they demonstrate the Royal Court indirectly adopting a contextual and/or purposive approach when construing the definition of a ‘beneficiary’ in the Trusts (Jersey) Law 1984, in order to meet the justice of the case.
Combinations of bacterial cultures, exogenous enzymes, and yeast-based feed additives and their impact on ruminal microbiome
Authors: Arce-Cordero J A , Bennett S L , Liu T , Ravelo A , Lobo R R , Jeong K C , Faciola A P 
Published: December 2022

Abstract
Our objective was to evaluate the effects of bacteria (Lactobacillus animalis, Propionibacterium freudenreichii, Bacillus lichenformis, Bacillus subtilis, and Enterococcus faecium), enzymes (amylase, hemicellulose, and xylanase), and yeast as additives on the ruminal microbiome. We hypothesized that inclusion of bacteria, enzymes, and yeast would impact butyric bacterial populations. Eight fermenters were arranged in a duplicated 4 × 4 Latin square with the following treatments: 1) control without additives (CTRL); 2) bacterial culture and enzyme blend (EB); 3) bacterial culture and enzyme blend with a live yeast and yeast culture blend (EBY); and 4) double dose of bacterial culture and enzyme blend and the yeast products blend (2X). We conducted four fermentation periods of 10 d each, with the last 3 d for collection of samples. Overall, 64 solid and liquid samples were analyzed by amplification of the V4 region of bacterial 16S rRNA. Data were analyzed with R and SAS. The following orthogonal contrasts were used: 1) ADD—the control compared to all treatments with additives (CTRL vs. EB, EBY, and 2X); 2) YEAST—treatment without yeast compared to those with yeast (EB vs. EBY and 2X); and 3) DOSE—the single dose of enzymes, bacteria, and yeast compared to the doubled dose (EBY vs. 2X). Family Prevotellaceae was more abundant when additives were added (ADD). Additives (ADD) also increased relative abundance of Prevotellaceae Ga6A1 and YAB2003 in solid fraction, and of Prevotellaceae Ga6A1 and two members of Lachnospiracea family in liquid fraction. Yeast (YEAST) decreased relative abundance of Succinivibrionaceae UCG-001 and increased abundance of Ruminococcus and Prevotellaceae UCG-003 in solid fraction. Doubling the dose of enzymes and microbial additives (DOSE) decreased the abundance of Succiniclasticum in solid fraction and Selenomonadaceae in the liquid. Molar proportion of butyrate was highly correlated with abundance of Prevotellaceae Ga6A1 in solid (r = 0.68) and liquid fraction (r = 0.79), and with Unclassified Lachnospiraceae in liquid (r = 0.70). Our results demonstrate that YEAST decreases abundance of succinate synthesizing bacteria, while DOSE decreases abundance of bacteria that metabolize succinate into propionate. Combined bacteria, enzymes, and yeast increase the relative abundance of specific genera primarily within the Prevotellaceae family, which may explain the increase in butyrate molar proportion observed with ADD.
Genome Sequence Resources for Verticillium dahliae-Inhibiting Streptomyces spp. Isolated from Agricultural Soil
Authors: LeBlanc Nicholas R. , Salgado-Salazar Catalina 
Published: December 2022

Gram-positive Streptomyces bacteria are common in soil, where they can inhibit soilborne plant pathogens by producing antimicrobial secondary metabolites. This study generated genome assemblies of Streptomyces isolates from soil that inhibit the soilborne plant pathogen Verticillium dahliae. Five bacterial isolates were cultured from soil collected from the Salinas Valley region of California. In vitro tests showed that three bacterial isolates inhibited V. dahliae growth when applied at low and high spore concentrations, whereas two isolates only inhibited V. dahliae when applied at high spore concentrations. Generation of hybrid Illumina-Nanopore assemblies showed that isolate genomes ranged from 7.45 to 8.95 Mbs. The contig number per assembly ranged from 1 to 54. All assemblies had 100% completeness values based on a standard set of conserved orthologs found in all bacteria. The number of predicted genes ranged from 6,594 to 8,098. Over 25 putative secondary metabolite biosynthetic clusters were identified in each assembly. Calculation of digital DNA:DNA hybridization and average nucleotide identity values using additional genome assemblies from type specimens of Streptomyces showed that the five isolates are likely novel Streptomyces spp. The results and data generated from this study will provide a basis for future research on the use of Streptomyces for managing common soilborne pathogens such as V. dahliae. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2023.
Oligometastatic disease from differentiated thyroid cancer: best treatment schemes
Authors: Colombo Elena , Ottini Arianna , Licitra Lisa 
Published: December 2022

Purpose of review
Patients with slowly progressive and/or symptomatic oligometastatic radioactive iodine refractory (RAIR) differentiated thyroid carcinomas (DTCs) are candidates to receive locoregional treatment to delay the start of systemic therapy with multikinase inhibitors. Information provided by the recent literature has not been extensively reviewed in previous published works, thus we aim to bridge this gap.

Recent findings
We present for each metastatic site the different locoregional treatment options, contraindications and potential adverse events. Some techniques can be combined together, whereas others are discouraged in certain situations, requiring a high level of expertise and multidisciplinarity in the treatment algorithm.

Summary
Different techniques of radiation therapy and interventional radiology allow to control the metastatic spread. However, as no clinical trials are available to compare the treatment schemes in terms of safety and potential impact on the prognosis, the most appropriate option for each patient should be selected within a multidisciplinary decision making, taking into account the clinical conditions and the pattern/rapidity of metastatic disease.
Post-COVID-19 syndrome in a sample of climacteric women living in Latin America
Authors: Blümel Juan Enrique , Vallejo María Soledad , Bencosme Ascanio , Calle Andrés , Dextre Maribel , Díaz Karen , López Marcela , Miranda Carlos , Ñañez Mónica , Ojeda Eliana , Rey Claudia , Rodrigues Marcio Alexandre Hipólito , Salinas Carlos , Tserotas Konstantinos , Aedo Sócrates , Pérez-López Faustino R. 
Published: December 2022

Abstract

Objective
The aim of this study was to assess resilience, fear of COVID-19, sleep disorders, and menopause-related symptoms after the acute phase of COVID-19 in middle-aged women with positive reverse transcription-polymerase chain reaction and noninfected women.

Methods
This is a cross-sectional, analytical study of climacteric women from 9 Latin American countries, aged 40-64 years, attending a routine health checkup. We evaluated clinical characteristics and used the Connor-Davidson Resilience Scale, the Fear of COVID-19 Scale, the Jenkins Sleep Scale, and the Menopause Rating Scale to evaluate their health.

Results
A total of 1,238 women were studied, including 304 who were positive for COVID-19 reverse transcription-polymerase chain reaction. The median (interquartile range) age was 53 (12) years; years of studies, 16 (6); body mass index, 25.6 (5.1) kg/m2; and time since first COVID-19 symptom, 8 (6) months. COVID-19 patients reported fatigability (18.8%), joint and muscular discomfort (14.1%), and anosmia (9.5%). They had a significantly lower resilience score (26.87 ± 8.94 vs 29.94 ± 6.65), higher Fear of COVID-19 score (17.55 ± 7.44 vs 15.61 ± 6.34), and a higher Jenkins Scale score (6.10 ± 5.70 vs 5.09 ± 5.32) compared with control women. A logistic regression model confirmed these results. There was not a significant difference in the total Menopause Rating Scale score, although the odds ratios for both severe menopausal symptoms (1.34; 95% confidence interval, 1.02-1.76) and the use of hypnotics were higher in women with COVID-19 (1.80; 95% confidence interval, 1.29-2.50) compared with those without infection. We found no decrease in studied outcomes between the initial 7 months versus those reported after 8 to 18 months since first COVID-19 symptoms.

Conclusions
COVID-19 climacteric women have sleep disorders, lower resilience and higher fear of COVID-19.
CircKlhl2 knockdown alleviates acute lung injury by regulating miR-29b-3p-mediated ROCK1 expression
Authors: Li Nan , Li Yuqiang , Wu Bin , Sun Rongli , Zhao Mingzhou , Hu Zhansheng 
Published: December 2022

Abstract

Background
Acute lung injury (ALI) induced by sepsis is distinguished by an inflammatory progression. Herein, we investigated the action of circular RNA kelch like family member 2 (circKlhl2) in sepsis-induced ALI.

Methods
The animal or cell model of sepsis-ALI was established by lipopolysaccharide (LPS) stimulation. The contents of circKlhl2, microRNA-29b-3p (miR-29b-3p), Rho associated coiled-coil containing protein kinase 1 (ROCK1), CyclinD1, B-cell lymphoma-2 (Bcl-2) and cleaved-caspase 3 (C-caspase3) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. Cell viability was assessed by Cell Counting Kit 8 (CCK8) assay. Cell cycle and apoptosis were evaluated by flow cytometry. The abundances of pro-inflammatory cytokines were detected by enzyme-linked immunosorbent assay (ELISA). Besides, the targeted relationship between miR-29b-3p and circKlhl2 or ROCK1 was verified by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay.

Results
Loss of circKlhl2 mitigated lung injury and pro-inflammatory cytokine expression in sepsis-ALI mice model and alleviated LPS-induced apoptosis and inflammatory response in MPVECs in vitro. The abundances of circKlhl2 and ROCK1 were boosted, while the miR-29b-3p level was diminished in the animal or cell model of sepsis-ALI. MiR-29b-3p inhibition abrogated circKlhl2 knockdown-mediated effects on MPVECs injury. Moreover, miR-29b-3p overexpression promoted cell proliferation and inhibited apoptosis and inflammation in LPS-treated MPVECs, while ROCK1 enhancement reversed these effects.

Conclusion
CircKlhl2 expedited the sepsis-induced acute lung injury by adjusting miR-29b-3p/ROCK1 axis.
SHORT-TERM INFLAMMATORY BIOMARKER PROFILES ARE ASSOCIATED WITH DEFICIENT MITOCHONDRIAL BIOENERGETICS IN LYMPHOCYTES OF SEPTIC SHOCK PATIENTS—A PROSPECTIVE COHORT STUDY
Authors: Nedel Wagner L. , Strogulski Nathan R. , Rodolphi Marcelo S. , Kopczynski Afonso , Montes Tiago H. M. , Portela Luis V. 
Published: December 2022

ABSTRACT

Introduction: A biomarker strategy based on the quantification of an immune profile could provide a clinical understanding of the inflammatory state in patients with sepsis and its potential implications for the bioenergetic state of lymphocytes, whose metabolism is associated with altered outcomes in sepsis. The objective of this study is to investigate the association between mitochondrial respiratory states and inflammatory biomarkers in patients with septic shock. Methods: This prospective cohort study included patients with septic shock. Routine, complex I, complex II respiration, and biochemical coupling efficiency were measured to evaluate mitochondrial activity. We measured IL-1ß, IL-6, IL-10, total lymphocyte count, and C-reactive protein levels on days 1 and 3 of septic shock management as well as mitochondrial variables. The variability of these measurements was evaluated using delta counts (days 3–1 counts). Results: Sixty-four patients were included in this analysis. There was a negative correlation between complex II respiration and IL-1ß (Spearman ρ, −0.275; P = 0.028). Biochemical coupling efficiency at day 1 was negative correlated with IL-6: Spearman ρ, −0.247; P = 0.05. Delta complex II respiration was negatively correlated with delta IL-6 (Spearman ρ, −0.261; P = 0.042). Delta complex I respiration was negatively correlated with delta IL-6 (Spearman ρ, −0.346; P = 0.006), and delta routine respiration was also negatively correlated with both delta IL-10 (Spearman ρ, −0.257; P = 0.046) and delta IL-6 (Spearman ρ, −0.32; P = 0.012). Conclusions: The metabolic change observed in mitochondrial complex I and complex II of lymphocytes is associated with a decrease in IL-6 levels, which can signal a decrease in global inflammatory activity.
Prevalence and Characteristics of Patients with Median Arcuate Ligament Syndrome in a Cohort Diagnosed with Celiac Artery Compression
Authors: Kozhimala Meagan , Chan Shin Mei , Weininger Gabe , Sumpio Brandon J , Levine Louis J , Harris Sean , Zheng Sijin , Longo Walter E , Chaar Cassius Ochoa , Guzman Raul J , Sumpio Bauer E 
Published: December 2022

Background:
Median arcuate ligament syndrome (MALS) is a frequent differential diagnosis in patients with post-prandial abdominal symptoms, but diagnosis remains challenging. The aim of this study was to identify characteristics of patients who had MALS compared to non-MALS patients amongst a cohort of patients diagnosed with celiac artery compression (CAC).

Study Design:
An IRB approved retrospective chart review (2000-2021) of patients at our institution with a discharge diagnosis of CAC was performed. Medical record review for clinical symptoms and findings consistent with MALS was performed.

Results:
293 patients with a diagnosis of CAC were identified. 59.7% were female and average age was 63.9 ± 20.2 years. 69 (23.5%) patients with CAC had MALS. There were no significant differences in sex or race between MALS and non-MALS patients, but MALS patients were younger (55.7 vs 68.1, p<0.001). There was no significant difference in gastrointestinal comorbidities between the two groups. Patients with MALS were less likely to have diabetes (12.5% vs. 26.9%), renal disease (4.6% vs 8.2%) and hypertension (41.5% vs. 70.3%), mesenteric atherosclerotic disease (14% vs 61.9%) and peripheral artery disease (15.0% vs 39.7%).

Conclusion:
We demonstrate a novel observation that MALS patients tend to have less atherosclerotic characteristics than non-MALS patients with CAC. Patients in our study with MALS were more likely to be younger, female, and presenting with epigastric pain. MALS patients had a significantly lower incidence of diabetes, hypertension, renal disease, mesenteric artery disease and peripheral arterial disease compared to the non-MALS group. An important clinically relevant feature of MALS patients may be their lack of atherosclerotic phenotype compared with non- MALS patients with CAC.
Increased migratory/activated CD8+ T cell and low avidity SARS-CoV-2 reactive cellular response in post-acute COVID-19 syndrome
Authors: Paniskaki Krystallenia , Konik Margarethe J. , Anft Moritz , Heidecke Harald , Meister Toni L. , Pfaender Stephanie , Krawczyk Adalbert , Zettler Markus , Jäger Jasmin , Gaeckler Anja , Dolff Sebastian , Westhoff Timm H. , Rohn Hana , Stervbo Ulrik , Scheibenbogen Carmen , Witzke Oliver , Babel Nina 
Published: December 2022

AbstractThe role of autoimmunity in post-acute sequelae of COVID-19 (PASC) is not well explored, although clinicians observe a growing population of convalescent COVID-19 patients with manifestation of post-acute sequelae of COVID-19. We analyzed the immune response in 40 post-acute sequelae of COVID-19 patients with non-specific PASC manifestation and 15 COVID-19 convalescent healthy donors. The phenotyping of lymphocytes showed a significantly higher number of CD8+ T cells expressing the Epstein-Barr virus induced G protein coupled receptor 2, chemokine receptor CXCR3 and C-C chemokine receptor type 5 playing an important role in inflammation and migration in PASC patients compared to controls. Additionally, a stronger, SARS-CoV-2 reactive CD8+ T cell response, characterized by IFNγ production and predominant TEMRAphenotype but low SARS-CoV-2 avidity was detected in PASC patients compared to controls. Furthermore, higher titers of several autoantibodies were detected among PASC patients. Our data suggest that a persistent inflammatory response triggered by SARS-CoV-2 might be responsible for the observed sequelae in PASC patients. These results may have implications on future therapeutic strategies.
Discordant results among MHC binding affinity prediction tools
Authors: Nguyen Austin , Nellore Abhinav , Thompson Reid F. 
Published: December 2022

ABSTRACTA large number of machine learning-based Major Histocompatibility Complex (MHC) binding affinity (BA) prediction tools have been developed and are widely used for both investigational and therapeutic applications, so it is important to explore differences in tool outputs. We examined predictions of four popular tools (netMHCpan, HLAthena, MHCflurry, and MHCnuggets) across a range of possible peptide sources (human, viral, and randomly generated) and MHC class I alleles. We uncovered inconsistencies in predictions of BA, allele promiscuity and the relationship between physical properties of peptides by source and BA predictions, as well as quality of training data. Our work raises fundamental questions about the fidelity of peptide-MHC binding prediction tools and their real-world implications.
Longitudinal improvement in postural instability is distinct from gait in treated adult hydrocephalus
Authors: Wacek Amber , Jean James , Jonason Alec , Hanson Jacob T. , Mavanji Vibha , Palzer Elise F. , Lewis Scott , Ashe James , Looft John M. , McGovern Robert A. 
Published: December 2022

AbstractBackgroundVentriculoperitoneal shunting improves gait in patients with normal pressure hydrocephalus. Postural instability is a major concern, but mostly ignored in the evaluation and treatment of these patients. This study quantified postural instability using kinematics via a prospective cohort design.MethodsSeventeen patients with suspected normal pressure hydrocephalus and twenty age-matched, healthy controls underwent quantitative pull test and gait examinations while wearing inertial measurement units at baseline. Patients with suspected normal pressure hydrocephalus who were shunted (n=13) and not shunted (n=4) underwent further testing after a lumbar drain trial and at follow-up visits 6 and 12 months post-operatively.ResultsWhile most gait improvement in patients who were shunted was seen immediately after the lumbar drain trial, measures of their postural response continued to improve after the lumbar drain trial through one year of follow-up. Patients who were not shunted showed no statistically significant changes in gait and postural instability measures.ConclusionsAfter shunting, postural instability improves continuously over one year. In contrast, a large improvement in gait is seen immediately with minimal change over the subsequent year. This difference in timing may implicate two distinct neurophysiological mechanisms of recovery and provides novel evidence that postural instability improves in response to long-term CSF diversion.