bluuebunny/crossref_metadata_2025_split huggingface dataset:
PD-L1-related LncRNAs are Associated with Immune Microenvironment and Prognosis in Glioma
Authors: Feng Jie , Tu Ruxin , Liu Fangkun , Zhang Hao , Dai Ziyu , Wang Zeyu , Luo Peng , Zhang Jian , Liu Zaoqu , Xiao Gelei , Xia Zhiwei , Cheng Quan 
Published: December 2022

Abstract
Background
The expression of long non-coding RNAs (lncRNAs) can function as diagnostic and therapeutic biomarkers of tumours, this research explored the effects of programmed cell death ligand-1(PD-L1) related lncRNAs on glioma.
Methods
Downloading gene expression profiles and clinicopathological information of glioma from TCGA and CGGA databases, 6 PD-L1-related lncRNAs were screened out through correlation analysis, Cox and LASSO regression analysis. The risk score model was established based on 6 PD-L1-related lncRNAs. Using GSVA and GSEA analyses to investigate the biological function. LINC01271 was selected as the target, and bioinformatics analysis and cell experiments in vitro were adopted to verify its effects on glioma.
Results
Risk scores based on 6 PD-L1-related lncRNAs (AL355974.3, LINC01271, AC011899.3, MIR4500HG, LINC02594, AL357055.3) can predict the prognosis of glioma(LGG and GBM). The high-risk score group has more typical malignant features in the immune-inflammatory microenvironment and is prone to be sensitive to anti-PD-1 treatment. The nomogram combining these lncRNAs and clinical parameters has good forecasting efficiency. LINC01271 expression can be used as a risk stratification index of glioma. Experiments in vitro confirmed its positive regulatory effect on the proliferation and migration of glioma cells.
Conclusions
This study demonstrates the predictive value of the risk score model based on 6 PD-L1-related lncRNAs for glioma characteristics, prognosis and immunotherapy responsiveness. LncRNA LINC01271 can independently be used as a new target for prognosis evaluation and therapy of glioma.
Comprehensive analysis of the prognosis and immune infiltration of TMC family members in renal clear cell carcinoma
Authors: Tang Wenbin , Shi Zhiyuan , Zhu Yasheng , Shan Zhengda , Jiang Aimin , Wang Anbang , Chen Ming , Bao Yi , Ju Guanqun , Xu Weidong , Wang Junkai 
Published: December 2022

Abstract
Renal cancer is a common malignancy of the urinary system, and renal clear cell carcinoma (RCCC) is the most common pathological type. Transmembrane channel-like (TMC) protein is an evolutionarily conserved gene family containing 8 members, however there is still a lack of comprehensive analysis about TMC family members in RCCC. In this study, we analyzed the expression of TMC family members in RCCC from TCGA and investigated the prognosis values and immune infiltration of TMC family members in RCCC. We found that TMC2, TMC3, TMC5, TMC7 and TMC8 were significantly related with overall survival (OS) of RCCC patients. TMC3, TMC6, and TMC8 was positively correlated with the degree of immune infiltration in RCCC. Thus, the TMC family members might be regarded as novel biomarkers for predicting potential prognosis and immunotherapy targets in RCCC patients.
Factors Associated with Long Term Outcomes and Transient Intraocular Pressure Elevation in Minimally Invasive Glaucoma Surgery Using Kahook Dual Blades
Authors: Sakamoto Tomoaki , Nisiwaki Hirokazu 
Published: December 2022

Abstract
In this retrospective case-control study, we aimed to investigate the mid- to long-term outcomes and factors involved in minimally invasive glaucoma surgery using the Kahook dual blade (KDB). Of the 229 cases in which KDB was introduced as the glaucoma surgery since 2018 at the Tenri Hospital, 133 eyes of 98 patients who could be followed for more than 3 months were included. Intraocular pressure (IOP), drop scores, and reoperation need were evaluated on day(s) 1, 3, 6, 9, 12, 24, and 36 months postoperatively. Significant differences in IOP and drop scores were observed between the preoperative and 12-month postoperative time points (P < 0.001). The amount of IOP change was related to preoperative and day 1 IOP. Moreover, the drop score changes were related to the preoperative drop scores and age. Multivariate analysis of Spike's incidence factors showed a significant association with axial length, preoperative drop scores, and day 1 IOP. These results suggest that KDB is effective in lowering IOP or decreasing the drop score. Special attention to postoperative spike occurrence may be recommended for patients with a long axial length, high drop scores, and high IOP on day 1.
External Debt, Renewable Energy, and Environmental Quality in BRICS Countries: Novel Findings Based on The Load Capacity Factor
Authors: Samour Ahmed , ADEBAYO Tomiwa Sunday 
Published: December 2022

Abstract

Since the introduction of the Sustainable Development Goals (SDGs), the BRICS nations have had difficulty in achieving the SDG targets, since sustaining ecological integrity has been a struggle for them. As a result, this research utilized a holistic indicator defined as load capacity factor to assess environmental quality derived from two major ecological elements: ecological footprints and bio-capacity. There is insufficient evidence in the available literature to investigate the variables influencing the load capacity factor in terms of environmental quality. From 1990 to 2018, this study examines the influence of external debt and renewable energy usage in improving the load capacity factor in BRICS nations. The research used sophisticated panel data estimates that can handle cross-sectional dependence and slope heterogeneity to assess the long-run relationship between the indicators. Furthermore, the study utilized MMQR to capture the association at different quantiles. The results illustrate that REC positively impacted the LCF in BRICS nations. In contrast, the findings illustrate that NREC, ED, and GDP adversely impact the LCF factor. These findings affirmed that REC positively and significantly influences promoting environmental sustainability in BRICS nations. While NREC, ED, and GDP have an adverse influence on the level of environmental sustainability. Furthermore, the MMQR results support both CCEMG and AMG long-run estimators. Based on these findings, the BRICS policymakers should design new policies to promote environmental sustainability by reinforcing the efficiency of energy systems and increasing the level of green energy investment.
ST3GalIV drives SLeX biosynthesis in gastrointestinal cancer cells and associates with cancer cell motility
Authors: Costa Ana Filipa , Senra Emanuel , Teixeira Andreia , Morais João , Pacheco Mariana , Faria-Ramos Isabel , Reis Celso A , Gomes Catarina 
Published: December 2022

Abstract
Expression of sialyl Lewis X (SLeX) is a well-documented event during malignant transformation of cancer cells, and largely associates with their invasive and metastatic properties. Glycoproteins and glycolipids are the main carriers of SLeX, whose biosynthesis is known to be performed by different glycosyltransferases, namely by the family of β-galactoside-α2,3-sialyltransferases (ST3Gals). In this study, we sought to elucidate the role of ST3GalIV in the biosynthesis of SLeX and in malignant properties of gastrointestinal (GI) cancer cells. By immunofluorescent screening, we selected SLeX-positive GI cancer cell lines and silenced ST3GalIV expression via CRISPR/Cas9. Immunofluorescence and western blot analysis showed that ST3GalIV KO efficiently impaired SLeX expression in most cancer cell lines, with the exception of the colon cancer cell line LS174T. The impact of ST3GalIV KO in the biosynthesis of SLeX isomer SLeA and non sialylated Lewis X and A were also evaluated and overall, ST3GalIV KO led to a decreased expression of SLeA and an increased expression in both LeX and LeA. In addition, the abrogation of SLeX on GI cancer cells led to a reduction in cell motility. Furthermore, ST3GalVI KO was performed in LS174T ST3GalIV KO cells, resulting in the complete abolishment of SLeX expression and consequent reduced motility capacity of those cells. Overall, these findings portray ST3GalIV as the main, but not the only, enzyme driving the biosynthesis of SLeX in GI cancer cells, with a functional impact on cancer cell motility.
Grid Connected Renewable Energy Source with Multi Objective Grey Wolf Optimization
Authors: Satyanarayana Vanam , Jayasankar Vairavasamy 
Published: December 2022

Abstract
Renewable energy sources like wind and solar will continue to grow in smart power systems through 2050, according to energy predictions. Due to the intermittent nature of these energy sources, using a grid entirely made of renewable resources is extremely unreliable and may result in certain operational problems. It is significantly important in applications and industry since the fundamental purpose of optimization is to find the best practical solution to a particular problem of interest. In this study, we suggest using the multi-objective Grey Wolf Optimizer (MOGWO), a more recent meta-heuristic that draws inspiration from the hunting habits and social structure of grey wolves, to optimize fuzzy controllers for mobile autonomous robots. The integration of storage facilities contributes to the upkeep of system balance Integration of storage facilities contributes to the preservation of system equilibrium. To analyze and discuss the proposed technique based on MOGWO and give simulation results for a two-wheeled autonomous mobile robot.
Heterogeneity of TPIT expression in ACTH-secreting extra-pituitary neuroendocrine tumors (NETs) supports the existence of different cellular programs in pancreatic and pulmonary NETs
Authors: Uccella Silvia , Leoni Eleonora , Kaiser Simon , Maragliano Roberta , Valerio Alessandro , Libera Laura , Tanda Maria Laura , Volante Marco , Diviani Dario , Rosa Stefano La 
Published: December 2022

Abstract
Extra-pituitary ACTH secretion is associated with a variety of neoplastic conditions and may cause the so-called ectopic ACTH-dependent Cushing syndrome (CS). The clarification of the mechanisms of extra-pituitary ACTH expression would provide potential therapeutic targets for this complex and severe disease. In the adenohypophysis, the transcription factor TPIT, co-operating with other molecules, induces POMC expression and ACTH production. However, no data is currently available on the presence and role of TPIT expression in extra-pituitary ACTH-producing neoplasms. This study was designed to explore TPIT expression in a series of pulmonary and pancreatic ACTH-producing tumors, either CS-associated or not.
41 extra-pituitary ACTH-producing neuroendocrine tumors (NETs) were included in the study, encompassing 32 NETs of the lung (LuNETs), 7 of the pancreas (PanNETs) and 2 pheochromocytomas. Of these, 9 LuNETs, all PanNETs and the two pheochromocytomas were CS-associated. For comparison, 6 NETs the pituitary gland (PitNETs), and 35 ACTH-negative NETs (15 Lu-NETs and 20 PanNETs) were analyzed. Immunohistochemistry with specific anti-TPIT antibodies and quantitative real time PCR (qRT-PCR) were performed using standard protocols.
TPIT expression was completely absent (protein and mRNA) in PanNETs, pheochromocytomas, and all ACTH-negative NETs. In contrast, it was expressed in 16/32 LuNETs, although with lower levels than in PitNETs. No definite relationship was found between immunohistochemistry TPIT expression and NET grade or the presence of Cushing syndrome.
This study further highlights the clinical and biological heterogeneity of extrapituitary ACTH secretion and suggests that the differences between ACTH-secreting PanNETs and LuNETs may mirror distinct molecular mechanisms underlying POMC expression. Our results point towards the recognition of a real corticotroph-like phenotype of ACTH-producing LuNETs, that is not a feature of ACTH-producing PanNETs.
U.S. Youth Use of the BIDI ® Stick Disposable E-Cigarette: The Importance of Establishing Device Specific Prevalence Data in Regulating Electronic Nicotine Delivery Systems (ENDS)
Authors: McKeganey Neil , Patton Andrea , Marza Venus , Barnard Gabriel 
Published: December 2022

Abstract
Background
In the light of the finding from the 2022 U.S. National Youth Tobacco Survey that disposable e-cigarette devices are the most widely used e-cigarette devices amongst U.S. youth use there is an important need to identify which disposable devices may be driving that increase. In this paper we report the results of research designed to estimate the prevalence of youth and underage young adult use of one of the leading disposable e-cigarette brands currently on sale within the U.S.
Methods
Crosssectional online survey of a nationally representative sample of 1,215 youth (1317 years) recruited via the IPSOS probability-based KnowledgePanel and 3,370 young adults aged 18 to 24 - amongst whom 1,125 were aged 18 to 20.
Results
Amongst youth aged 13 to 17, 0.91% [95% CI: 0.44–1.68] reported having ever used a BIDI® Stick branded product and 0.04% [95% CI: 0.00-0.38] reported currently using a BIDI® Stick branded product. Amongst those young adults aged 18 to 20, 3.90% [95% CI: 2.49–5.81] reported having ever used a BIDI® Stick product whilst 0.60% [95% CI: 0.17–1.55] reported they now use a BIDI® Stick product “every day” or “some days”.
Conclusions
The low prevalence of youth and underage adult current use of the BIDI® Stick ecigarette suggests that this product is not responsible for the recent growth in the use of disposable e-cigarettes by youth within the U.S. as demonstrated by the 2022 National Youth Tobacco Survey.
Comparison of physicochemical characteristics and biological activities of polysaccharides from Lycium barbarum L. at different tree ages
Authors: Wang Ningli , Wang Han , Meng Hao , Guo Xinyu , Pei Dong , Yang Yingli , Liu Jianfei , Di Duolong 
Published: December 2022

AbstractThe biological activity is related to the content of active substances, and the accumulation of active substances is closely related to the tree age. The relationship between the physicochemical characteristics and biological activities ofLycium barbarumpolysaccharides (LBPs) and tree ages was studied in this study. The physicochemical characteristics of LBPs at second (Y2), fourth (Y4), sixth (Y6), eighth (Y8), tenth (Y10) and fifteenth (Y15) tree ages, such as total carbohydrate content (TCC), protein content (PC), uronic acid content (UAC), monosaccharide composition, molecular weight distribution, infrared characteristics were determined. And effects of LBPs of different tree ages on PC12 cells and APRE-19 cells function were evaluated in vitro. The results indicated that there was no significant difference in PC of LBPs at different tree ages. The UAC and TCC of LBPs from Y4 to Y10 were not significantly different, but were significantly higher than those in Y2 and Y15. LBPs of different tree ages showed similar preliminary structural characteristics. Compared with the LBPs of other tree ages, the LBPs of Y4, Y6, Y8 and Y10 had a good role in resisting oxidative stress. These results indicated that molecular weight, TCC and UAC of LBPs had important effects on their antioxidant activity in vitro. The accumulation of UAC and TCC in LBPs was closely related to tree age. The physicochemical characteristics and biological activities of LBPs fromY4 to Y10 were better than Y2 and Y15. Therefore,Lycium barbarumL. from Y4 to Y10 should be selected when preparing LBPs.
Design and Implementation of a Real-Time Face Recognition System Based on Artificial Intelligence Techniques
Authors: Chang Chih-Yung , santra Arpita Samanta , Chang I-Hsiung , Wu Shih-Jung , Roy Diptendu Sinha 
Published: December 2022

Abstract
This paper mainly discusses the asymmetric face recognition problem where the number of names in a name list and the number of faces in the photo might not be equal, but each face should be automatically labeled with a name. The motivation for this issue is that there had been many meetings in the past. After each meeting, the participant took group photos. The meeting provided only a corresponding name list of participants without one-to-one labels. In the worst case, the group photo might mix with the faces that were not participating in the meeting. Another reason for asymmetric face recognition is that some meeting personnel did not appear in photos because they assisted in taking pictures. This paper proposes an Asymmetric Face Recognition Mechanism, called AFRM in short. Initially, the proposed AFRM adopts the Histogram of Oriented Gradients (HOG) and Support Vector Machine (SVM) to detect and extract all faces from photos. Next, AFRM extracts the features from each face using the convolution feature map (Conv_FF) and adopts the features to partition the faces into different classes. Then the AFRM applies the statistic-based mechanism to map each name in the name list to each face class. According to this mapping, each face will be associated with one name. To quickly identify a face during the meeting, the AFRM applies the K-Nearest Neighbors (KNN) to represent the features of each face. During the new meeting, the proposed AFRM can extract the feature of one face and then adopts KNN to derive the features. Experimental results show that the proposed mechanism achieves more than 97% accuracy without one-to-one name and face labeling.
Native T1 high region and left ventricular ejection fraction recovery in patients with dilated cardiomyopathy
Authors: Nabeta Takeru , Takigami Yu , Eda Yuko , Maemura Kenji , Oki Takumi , Fujita Teppei , Iida Yuichiro , Ikeda Yuki , Ishii Shunsuke , Inoue Yusuke , Ako Junya , Yazaki Mayu 
Published: December 2022

Abstract
Native T1 mapping is used to assess myocardial tissue characteristics without gadolinium contrast agents. The focal T1 high-intensity region can indicate myocardial alterations. This study aimed to identify the association between the native T1 mapping including the native T1 high region and left ventricular ejection fraction (LVEF) recovery in patients with dilated cardiomyopathy (DCM). Patients with newly diagnosed DCM (LVEF of < 45%) who underwent cardiac magnetic resonance imaging with native T1 mapping were included in the analysis. Native T1 high region was defined as a signal intensity of > 5 SD in the remote myocardium. Recovered EF was defined as a follow-up LVEF of ≥ 45% and an LVEF increase of ≥ 10% after 2 years from baseline. Seventy-one patients met the inclusion criteria for this study. Forty-four patients (61.9%) achieved recovered EF. Logistic regression analysis showed that the native T1 value (OR: 0.98; 95% CI: 0.96–0.99; P = 0.014) and the native T1 high region (OR: 0.17; 95% CI: 0.05–0.55; P = 0.002), but not late gadolinium enhancement, were independent predictors of recovered EF. Compared with native T1 value alone, combined native T1 high region and native T1 value improved the area under the curve from 0.703 to 0.788 for predicting recovered EF. Myocardial damage, which was quantified using native T1 mapping and the native T1 high region were independently associated with recovered EF in patients with newly diagnosed DCM.
Evaluation of stability of mainstream ANAMMOX: Long-term operated A3O process
Authors: Lee Jiwon , Lee Woo Hyoung , Bae Sungwoo , Gil Kyungik 
Published: December 2022

Abstract
The stability of the ANAMMOX (Anaerobic ammonium oxidation) process for the side-stream has been proven worldwide, but the stability for the mainstream has not yet been proven. Most studies in previous mainstream ANAMMOX research have only focused on nitritating ammonium nitrogen at a low concentration. This study did not nitritate low-concentration nitrogen during the mainstream ANAMMOX process, but changed the idea to bringing the nitrite source from the recycle water. Since the recycle water contains a high concentration of nitrogen, nitritation reaction is stably induced, and the nitrite nitrogen is used as a substrate source necessary for the mainstream ANAMMOX reaction. In this study, the results of about 1500 days of successful operation at the lab scale were summarized, and the necessary operating factors and data were successfully derived at the real scale.
What is the impact of post-COVID-19 syndrome on health-related quality of life and associated factors: a cross-sectional analysis
Authors: Mastrorosa Ilaria , Duca Giulia Del , Pinnetti Carmela , Lorenzini Patrizia , Vergori Alessandra , Brita Anna Clelia , Camici Marta , Mazzotta Valentina , Baldini Francesco , Chinello Pierangelo , Mencarini Paola , Giancola Maria Letizia , Abdeddaim Amina , Girardi Enrico , Vaia Francesco , Antinori Andrea 
Published: December 2022

Abstract
Background.
After the acute phase, symptoms or sequelae related to post-COVID-19 syndrome may persist for months. We aim to measure their impact on health-related quality of life (HRQoL) and to investigate influencing factors, among patients, previously hospitalized and not, followed up to 12 months.
Methods.
We present the cross-sectional analysis of a prospective study, including patients referred to the post-COVID-19 service. Questionnaires and scales administered at 3, 6, 12 months were: Short-Form 36-item questionnaire (SF-36); Visual Analogue Scale of the EQ5D (EQ-VAS); in a subgroup, Beck Anxiety Inventory (BAI), Beck Depression Inventory (BDI-II) and Pittsburgh Sleep Quality Index (PSQI). Linear regression was used to assess for the presence of a trend over time by month of evaluation after the acute infection (continuous measure in month); two different linear regression models were fitted to identify factors associated with HRQoL.
Results.
We considered the first assessment of each participant (n = 572): 235 (41%), 175 (31%) and 118 (21%) patients were evaluated 3 months [median 3.5 (IQR 2.9-3-9)], 6 months [5.6 (5.0-6.5)], and more than 6 months [9.6 (8.3–14.7)], after the acute infection, respectively. The mean scores in SF-36 and in EQ-VAS were significantly lower than the Italian normative values and remained stable over time, except the mental components score (MCS) of the SF-36 and EQ-VAS which resulted in lower ratings at the last observations. Female gender, presence of comorbidities, and corticosteroids treatment during acute COVID-19, were associated with lower scores in SF-36 and EQ-VAS; patients previously hospitalized (54%) reported higher MCS. Alterations in BAI, BDI-II, and PSQI, evaluated in a subgroup of 265 participants, were associated with lower ratings in SF-36 and EQ-VAS.
Conclusions.
This study provides evidence of a significantly bad perception of health status among persons with post-COVID-19 syndrome, associated with female gender and, indirectly, with disease severity. In case of anxious-depressive symptoms and sleep disorders, a worse HRQoL was also reported. A systematic monitoring of these aspects is recommended to properly manage the post-COVID-19 period.