bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Untargeted LC–MS based approach for identification of altered metabolites in blood plasma of rheumatic heart disease patients
Authors: Das Soumi , Kumar Yashwant , Sharma Shruti , Ray Ruma , Arava Sudheer , Seth Sandeep , Agarwal Aman , Sharma Gautam 
Published: March 2022

AbstractRheumatic heart disease (RHD) is often considered as a disease of developing countries and India is the home of about 40% of RHD patients. Environment seems to play a major role in its causation. Since gene environment interactions can lead to alterations of various metabolic pathways, identification of altered metabolites can help in understanding the various pathways leading to RHD. Blood plasma samples from 51 RHD and 49 healthy controls were collected for the study. Untargeted metabolomics approach was used to identify the metabolites that are altered in RHD patients. Data showed 25 altered metabolites among RHD patients. These altered metabolites were those involved in Purine, Glutamine, Glutamate, Pyrimidine, Arginine, Proline and Linoleic metabolism. Thus, the present study illuminates metabolic alterations among RHD patients which can help in determining the potential therapeutic targets.
Microbial differences between active and remission peri-implantitis
Authors: Hashimoto Yuhei , Okada Shinsuke , Yasuda Keisuke , Kawagoe Maiko , Kajiya Mikihito , Tsuga Kazuhiro 
Published: March 2022

AbstractPeri-implantitis has a polymicrobial etiology and is a major cause of dental implant loss. Various clinical protocols for its prevention and treatment have been proposed; however, some cases show a rapid progression with non-resolving clinical symptoms. To clear a means of differentiating between such cases, the implants with peri-implantitis in this study were categorized as the active group and the remission group and that two kinds of samples were obtained from the same subjects (n = 20). The microbiome was analyzed through pyrosequencing of the 16S rRNA gene. From LEfSe results, Porphyomonas, Fusobacterium, Treponema, Tannerella, and other periodontal pathogens were abundant in the active group, while lactic acid bacteria (Lactobacillales and Bifidobacterium) were abundant in the remission group.
Sex differences in esophageal cancer overall and by histological subtype
Authors: Stabellini Nickolas , Chandar Apoorva Krishna , Chak Amitabh , Barda Amie J. , Dmukauskas Mantas , Waite Kristin , Barnholtz-Sloan Jill S. 
Published: March 2022

AbstractEsophageal cancer is the seventh most common type of cancer in the world, the sixth leading cause of cancer-related death and its incidence is expected to rise 140% in the world in a period of 10 years until 2025. The overall incidence is higher in males, while data about prognosis and survival are not well established yet. The goal of this study was to carry out a comprehensive analysis of differences between sexes and other covariates in patients diagnosed with primary esophageal cancer. Data from 2005 to 2020 were obtained from the University Hospitals (UH) Seidman Cancer Center and from 2005 to 2018 from SEER. Patients were categorized according to histological subtype and divided according to sex. Pearson Chi-square test was used to compare variables of interest by sex and the influence of sex on survival was assessed by Kaplan Meier, log rank tests and Cox proportional hazards regression models. A total of 1205 patients were used for analysis. Sex differences in all types were found for age at diagnosis, histology, smoking status and prescriptions of NSAIDs and in SCC for age at diagnosis and alcoholism. Survival analysis didn’t showed differences between males and females on univariable and multivariable models. Males have a higher incidence of Esophageal Cancer and its two main subtypes but none of the comprehensive set of variables analyzed showed to be strongly or unique correlated with this sex difference in incidence nor are they associated with a sex difference in survival.
Cytotoxicity, early safety screening, and antimicrobial potential of minor oxime constituents of essential oils and aromatic extracts
Authors: Surowiak Alicja Karolina , Sowała Marta , Talma Michał , Groborz Katarzyna , Balcerzak Lucyna , Lochyński Stanisław , Strub Daniel Jan 
Published: March 2022

AbstractDue to market and legislative expectations, there is a constant need to explore new potential antimicrobial agents for functional perfumery. In this study, we evaluated the antimicrobial activity of 53 low molecular oximes and the corresponding carbonyl compounds against Escherichia coli, Enterococcus hirae, Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, Aspergillus brasiliensis, Legionella pneumophila and Candida albicans. The most potent compound was α-isomethylionone oxime, which exhibited a minimum inhibitory concentration (MIC) of 18.75 µg/mL against E. hirae. The evaluation of the MICs for bacterial and fungal strains was performed for selected compounds, for example, the MIC of 2-phenylpropionaldehyde, cis-jasmone oxime, and trans-cinnamaldehyde measured against A. brasiliensis was 37.50 µg/mL. ADME-Tox (Absorption, Distribution, Metabolism, Excretion, and Toxicity) and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assays were performed to assess the cytotoxicity of tested compounds. ADME-Tox indicated the safety and promising properties of selected compounds, which enables their usage as nontoxic supporting antibacterial agents. The results of the in vitro MTS assay were consistent with the ADME-Tox results. None of the compounds tested was toxic to Human Embryonic Kidney 293T (HEK293T) cells, with all cell viabilities exceeding 85%.
Big data analysis of the risk factors and rates of perioperative transfusion in immediate autologous breast reconstruction
Authors: Song Woo Jin , Kim Hee Jin , Kang Sang Gue , Seo Bommie Florence , Choi Nam Kyong , Lee Jung Ho 
Published: March 2022

AbstractPatients undergoing autologous breast reconstruction (ABR) are more likely to require perioperative transfusions due to the increased intraoperative bleeding. In addition to the mastectomy site, further incisions and muscle dissection are performed at the donor sites, including the back or abdomen, increasing the possibility of transfusion. The purpose of this study was to evaluate perioperative transfusion rates and risk factors according to the type of ABR through analysis of big data. Patients who underwent total mastectomy for breast cancer between 2014 and 2019 were identified. The patients were divided into mastectomy only and immediate ABR groups. The transfusion rate was 14-fold higher in the immediate ABR group (16.1%) compared to the mastectomy only group (1.2%). The transfusion rate was highest with the pedicled transverse rectus abdominis myocutaneous flap (24.2%). Performance of the operation in medical institutions located in the provinces and coronary artery disease (CAD) were significant risk factors for the need for transfusion. The perioperative transfusion risk among patients undergoing immediate ABR was related to the flap type, location of medical institution, and CAD. Based on the higher transfusion rate in this study (16.1%) compared to previous studies, the risk factors for the need for transfusion should be determined and evidence-based guidelines should be developed to reduce the transfusion rates.
Alterations in common marmoset gut microbiome associated with duodenal strictures
Authors: Sheh Alexander , Artim Stephen C. , Burns Monika A. , Molina-Mora Jose Arturo , Lee Mary Anne , Dzink-Fox JoAnn , Muthupalani Sureshkumar , Fox James G. 
Published: March 2022

AbstractChronic gastrointestinal (GI) diseases are the most common diseases in captive common marmosets (Callithrix jacchus). Despite standardized housing, diet and husbandry, a recently described gastrointestinal syndrome characterized by duodenal ulcers and strictures was observed in a subset of marmosets sourced from the New England Primate Research Center. As changes in the gut microbiome have been associated with GI diseases, the gut microbiome of 52 healthy, non-stricture marmosets (153 samples) were compared to the gut microbiome of 21 captive marmosets diagnosed with a duodenal ulcer/stricture (57 samples). No significant changes were observed using alpha diversity metrics, and while the community structure was significantly different when comparing beta diversity between healthy and stricture cases, the results were inconclusive due to differences observed in the dispersion of both datasets. Differences in the abundance of individual taxa using ANCOM, as stricture-associated dysbiosis was characterized byAnaerobiospirillumloss andClostridium perfringensincreases. To identify microbial and serum biomarkers that could help classify stricture cases, we developed models using machine learning algorithms (random forest, classification and regression trees, support vector machines and k-nearest neighbors) to classify microbiome, serum chemistry or complete blood count (CBC) data. Random forest (RF) models were the most accurate models and correctly classified strictures using either 9 ASVs (amplicon sequence variants), 4 serum chemistry tests or 6 CBC tests. Based on the RF model and ANCOM results,C. perfringenswas identified as a potential causative agent associated with the development of strictures.Clostridium perfringenswas also isolated by microbiological culture in 4 of 9 duodenum samples from marmosets with histologically confirmed strictures. Due to the enrichment ofC. perfringensin situ, we analyzed frozen duodenal tissues using both 16S microbiome profiling and RNAseq. Microbiome analysis of the duodenal tissues of 29 marmosets from the MIT colony confirmed an increased abundance ofClostridiumin stricture cases. Comparison of the duodenal gene expression from stricture and non-stricture marmosets found enrichment of genes associated with intestinal absorption, and lipid metabolism, localization, and transport in stricture cases. Using machine learning, we identified increased abundance ofC. perfringens, as a potential causative agent of GI disease and intestinal strictures in marmosets.
Western boundary currents drive sun-coral (Tubastraea spp.) coastal invasion from oil platforms
Authors: Coelho Stella Correia Cesar , Gherardi Douglas Francisco Marcolino , Gouveia Mainara Biazati , Kitahara Marcelo Visentini 
Published: March 2022

AbstractMost marine species have a planktonic larval phase that benefit from the surface oceanic flow to enhance their dispersion potential. For invasive species, the interaction of environmentally resistant larvae with different flow regimes and artificial substrates can lead to complex larval dispersion patterns and boost geographic expansion. In the Southwest Atlantic, the invasive corals Tubastraea spp. (sun-coral) have been recorded biofouling on oil platforms since the late 1980s. These platforms are considered important vectors for the established populations throughout the Brazilian coast. However, we still do not know how the position of these structures relative to regional flow contribute to the natural dispersion potential of these invaders on a regional scale. Herein, we used an eddy-resolving ocean model (ROMS) and an Individual Based Model (IBM-Ichthyop) to simulate the natural dispersion patterns of sun-coral larvae from all oil platforms on Brazilian oil-producing basins, for the austral summer and winter along 6 years (2010–2015) in 90-day simulations. We found that mortality rates by advection were significantly higher during the winter (p = 0.001) and when sources of larvae were compared throughout this season (p = 1.9 × 10–17). The influence of two western boundary currents and persistent eddy activity contribute to the dispersal of larvae to distances up to 7000 km. The effectiveness of each oil-producing basin as vectors for the entire Brazilian coastline, measured as the percentage of larval supply, highlights the importance of the northern Ceará (59.89%) and Potiguar (87.47%) basins and the more central Camamu (44.11%) and Sergipe-Alagoas (39.20%) basins. The poleward shift of the Southern branch of the South Equatorial Current during the winter causes larvae released from the Sergipe-Alagoas and Camamu basins to enter the North Brazil Current, expanding their dispersion towards the north. The Brazil Current disperses larvae southwards, but strong mesoscale activity prevents their dispersion to the coast, especially for those released from the oil platforms on Campos and Santos basins. Within this complex hydrodynamic setting, a few source areas, like those in the Sergipe-Alagoas and Camamu basins, can potentially contribute to the spread of larvae along nearly all the Brazilian coast. Therefore, oil platforms act as possible chronic sources of sun-coral propagules to the coast, emphasizing the urgency for a more detailed set of actions to control and monitor these invasive exotic species.
Aerosol emissions and gravity waves of Taal volcano
Authors: Nee Jan-Bai , Chang Yuan-Pin , Wang Chia C. 
Published: March 2022

AbstractThe Taal volcano (14.0 N, 121.0 E) in Philippines erupted in January–February 2020, with a part of aerosols drifted northward and detected by a lidar system at Kaohsiung city (22.37 N, 120.15 E), Taiwan. The aerosol observed on Feb 11 is special for its high-altitude distributions at 4–7 km with discrete structures which can be resolved into a sinusoidal oscillation of ~ 30 min period, suggesting a case of wave event caused by the eruptions. We report in this paper the gravity wave generated by the volcanic eruptions and its effects on aerosol emissions. By studying the temperature and pressure data in the Taal region using radiosonde data, we found atmospheric gravity waves with powers correlated with the optical thickness (AOD) at 550 nm measured by the Moderate Resolution Imaging Spectrometer (MODIS) satellite. This study presents the first observation of modulation of the aerosol emissions by the volcanic gravity waves and a case of coupling of dynamics and chemistry.
More tropical cyclones are striking coasts with major intensities at landfall
Authors: Wang S. , Toumi R. 
Published: March 2022

AbstractIn this study, we show that the number of annual global tropical cyclone (TC) landfalls with major landfall intensity (LI ≥ 50 m s−1) has nearly doubled from 1982 to 2020. The lifetime maximum intensity (LMI) of global major landfalling TCs has been increasing by 0.8 m s−1per decade (p < 0.05), but this significance of intensity change disappears at landfall (0.3 m s−1per decade,p = 0.69). The lack of a significant LI trend is caused by the much larger variance of LI than that of LMI in all basins and explains why a significant count change of TCs with major intensity at landfall has only now emerged. Basin-wide TC trends of intensity and spatial distribution have been reported, but this long-term major TC landfall count change may be the most socio-economic significant.
Multimodal bioimaging using nanodiamond and gold hybrid nanoparticles
Authors: Lin Yu-Chung , Perevedentseva Elena , Lin Zhe-Rui , Chang Chia-Chi , Chen Hsiang-Hsin , Yang Shun-Min , Lin Ming-Der , Karmenyan Artashes , Speranza Giorgio , Minati Luca , Nebel Christoph , Cheng Chia-Liang 
Published: March 2022

AbstractHybrid core–shell nanodiamond-gold nanoparticles were synthesized and characterized as a novel multifunctional material with tunable and tailored properties for multifunctional biomedical applications. The combination of nanostructured gold and nanodiamond properties afford new options for optical labeling, imaging, sensing, and drug delivery, as well as targeted treatment. ND@Au core–shell nanoparticles composed of nanodiamond (ND) core doped with Si vacancies (SiV) and Au shell were synthesized and characterized in terms of their biomedical applications. Several bioimaging modalities based on the combination of optical and spectroscopic properties of the hybrid nano-systems are demonstrated in cellular and developing zebrafish larvae models. The ND@Au nanoparticles exhibit isolated ND’s Raman signal of sp3 bonded carbon, one-photon fluorescence of SiV with strong zero-phonon line at 740 nm, two-photon excited fluorescence of nanogold with short fluorescence lifetime and strong absorption of X-ray irradiation render them possible imaging agent for Raman mapping, Fluorescence imaging, two-photon Fluorescence Lifetime Imaging (TP-FLIM) and high-resolution hard-X-ray microscopy in biosystems. Potential combination of the imaging facilities with other theranostic functionalities is discussed.
Altered electrical properties in skeletal muscle of mice with glycogen storage disease type II
Authors: Nagy Janice A. , Semple Carson , Riveros Daniela , Sanchez Benjamin , Rutkove Seward B. 
Published: March 2022

AbstractElectrical impedance methods, including electrical impedance myography, are increasingly being used as biomarkers of muscle health since they measure passive electrical properties of muscle that alter in disease. One disorder, Pompe Disease (Glycogen storage disease type II (GSDII)), remains relatively unstudied. This disease is marked by dramatic accumulation of intracellular myofiber glycogen. Here we assessed the electrical properties of skeletal muscle in a model of GSDII, the Pompe6neo/6neo (Pompe) mouse. Ex vivo impedance measurements of gastrocnemius (GA) were obtained using a dielectric measuring cell in 30-week-old female Pompe (N = 10) and WT (N = 10) mice. Longitudinal and transverse conductivity, σ, and the relative permittivity, εr, and Cole–Cole complex resistivity parameters at 0 Hz and infinite frequency, ρo and ρ∞, respectively, and the intracellular resistivity, ρintracellular were determined from the impedance data. Glycogen content (GC) was visualized histologically and quantified biochemically. At frequencies > 1 MHz, Pompe mice demonstrated significantly decreased longitudinal and transverse conductivity, increased Cole–Cole parameters, ρo and ρo-ρ∞, and decreased ρintracellular. Changes in longitudinal conductivity and ρintracellular correlated with increased GC in Pompe animals. Ex vivo high frequency impedance measures are sensitive to alterations in intracellular myofiber features considered characteristic of GSDII, making them potentially useful measures of disease status.
Ultrasonic bone age fractionates cognitive abilities in adolescence
Authors: Kovács Ilona , Kovács Kristóf , Gerván Patrícia , Utczás Katinka , Oláh Gyöngyi , Tróznai Zsófia , Berencsi Andrea , Szakács Hanna , Gombos Ferenc 
Published: March 2022

AbstractAdolescent development is not only shaped by the mere passing of time and accumulating experience, but it also depends on pubertal timing and the cascade of maturational processes orchestrated by gonadal hormones. Although individual variability in puberty onset confounds adolescent studies, it has not been efficiently controlled for. Here we introduce ultrasonic bone age assessment to estimate biological maturity and disentangle the independent effects of chronological and biological age on adolescent cognitive abilities. Comparing cognitive performance of female participants with different skeletal maturity we uncover the impact of biological age on both IQ and specific abilities. We find that biological age has a selective effect on abilities: more mature individuals within the same age group have higher working memory capacity and processing speed, while those with higher chronological age have better verbal abilities, independently of their maturity. Based on our findings, bone age is a promising biomarker of adolescent maturity.
Crash test-based assessment of injury risks for adults and children when colliding with personal mobility devices and service robots
Authors: Paez-Granados Diego , Billard Aude 
Published: March 2022

AbstractAutonomous mobility devices such as transport, cleaning, and delivery robots, hold a massive economic and social benefit. However, their deployment should not endanger bystanders, particularly vulnerable populations such as children and older adults who are inherently smaller and fragile. This study compared the risks faced by different pedestrian categories and determined risks through crash testing involving a service robot hitting an adult and a child dummy. Results of collisions at 3.1 m/s (11.1 km/h/6.9 mph) showed risks of serious head (14%), neck (20%), and chest (50%) injuries in children, and tibia fracture (33%) in adults. Furthermore, secondary impact analysis resulted in both populations at risk of severe head injuries, namely, from falling to the ground. Our data and simulations show mitigation strategies for reducing impact injury risks below 5% by either lowering the differential speed at impact below 1.5 m/s (5.4 km/h/3.3 mph) or through the usage of absorbent materials. The results presented herein may influence the design of controllers, sensing awareness, and assessment methods for robots and small vehicles standardization, as well as, policymaking and regulations for the speed, design, and usage of these devices in populated areas.
Sentinel-1A for monitoring land subsidence of coastal city of Pakistan using Persistent Scatterers In-SAR technique
Authors: Hussain Muhammad Afaq , Chen Zhanlong , Shoaib Muhammad , Shah Safeer Ullah , Khan Junaid , Ying Zheng 
Published: March 2022

AbstractKarachi is located in the southern part of Pakistan along the Arabian Sea coast. Relevant institutions are concerned about the possibility of ground subsidence in the city, contributing to the comparative sea-level rise. So yet, no direct measurement of the subsidence rate and its relation to city submergence danger has been made. SAR (Synthetic Aperture Radar) interferometry is a powerful method for obtaining millimeter-accurate surface displacement measurements. The Sentinel-1 satellite data provide extensive geographical coverage, regular acquisitions, and open access. This research used the persistent scatterer interferometry synthetic aperture radar (PS-InSAR) technology with Sentinel-1 SAR images to monitor ground subsidence in Karachi, Pakistan. The SARPROZ software was used to analyze a series of Sentinel-1A images taken from November 2019 to December 2020 along ascending and descending orbit paths to assess land subsidence in Karachi. The cumulative deformation in Line of Sight (LOS) ranged from − 68.91 to 76.06 mm/year, whereas the vertical deformation in LOS ranged from − 67.66 to 74.68 mm/year. The data reveal a considerable rise in subsidence from 2019 to 2020. The general pattern of subsidence indicated very high values in the city center, whereas locations outside the city center saw minimal subsidence. Overall, the proposed technique effectively maps, identifies, and monitors land areas susceptible to subsidence. This will allow for more efficient planning, construction of surface infrastructure, and control of subsidence-induced risks.