bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Novel Role of Midbody-Associated mRNAs in Regulating Abscission
Authors: Farmer Trey , Han Ke-Jun , Vaeth Katherine , Taliaferro Matthew , Prekeris Rytis 
Published: October 2022

Abstract
Midbodies (MBs) have been shown to function during telophase as a recruiting hub, especially for ESCRT-III complex subunits, including CHMP4B, to regulate the abscission step of cytokinesis. However, the molecular machinery governing specific protein targeting and activation at the MB remains poorly understood. Until recently, it was thought that abscission regulating proteins, such as ESCRT-III complex subunits, accumulate at the MB by directly or indirectly binding to the MB resident protein, CEP55. However, recent studies have shown that depletion of CEP55 does not fully block ESCRT-III targeting to the MB, and cells in CEP55 knock-out mice divide normally. Additionally, since MBs are microtubule-rich, proteinaceous structures, it is conceptually hard to imagine how large protein complexes, such as the ESCRT-III complex, can successfully diffuse into the MB from the cytosol in a rapid and highly regulated manner. Here, we show that MBs contain mRNAs and that these MB-associated mRNAs can be locally translated, thus, resulting in the accumulation of abscission-regulating proteins. We also demonstrate that localized MB-associated translation of CHMP4B is required for it’s targeting to the abscission site. Finally, we demonstrate that 3’-UTR-dependent CHMP4B mRNA targeting to the MB is required for successful completion of cytokinesis. Based on all this data, we propose a novel method of regulating cytokinesis and abscission by MB-associated targeting and localized translation of selective mRNAs.
An Improved Route to the Regioselective Synthesis of 1,7-dibenzyl-1,4,7,10-tetraazacyclododecane
Authors: Zhang Xiaoting , Li Huirui , Kong Dehao , Wan Fuxian 
Published: October 2022

Abstract
1,7-dibenzyl-1,4,7,10-tetraazacyclododecane is a crucial intermediate in the preparation of tetra-armed 1,4,7,10-tetraazacyclododecane (two benzyl groups at N1- and N7- positions and two other aromatic groups at N4- and N10- positions), 1,7-disubstituted-1,4,7,10-tetraazacyclododecane, and DO2A metal chelators, which find widespread use in medicine. Current frequently-used synthesis is costly and involves multi-steps under harsh reaction conditions, delivering low yields. We present a simpler, highly regioselective synthesis of the 1,7-dibenzyl-1,4,7,10-tetraazacyclododecane that operates under mild reaction conditions.
Aromatic plants rich in phenolic compounds as a valuable basis for dietary supplements in intestinal motility disorders
Authors: García-Giménez M.D. , Quilez-Guerrero A. , Fernandez-Arche M.A. , Puerta R. De la 
Published: October 2022

Abstract
Plant extracts can be an important adjuvant treatment in gastrointestinal diseases where intestinal transit is involved. Because transit disorders are often associated with infections and inflammation, in our investigation we have selected five aromatic Mediterranean plants with antimicrobial, antioxidant and anti-inflammatory activities which in certain areas are also used for culinary reasons. We evaluated the intestinal transit in mice after oral administration of hydro-alcoholic extracts of Achillea millefolium L. Sideritis angustifolia Lag., Rosmarinus officinalis L. Matricaria chamomile L., and Aloysia citriodora Palau. Total content of phenols and flavonoids and their antioxidant activity were previously determined. Rosmarinus officinalis showed the highest antioxidant capacity (p < 0.001) in the DPPH and ABTS methods with IC50 of 48.89 ± 2.98 and 27.28 ± 1.83 µg/mL respectively, in agreement with the highest phenol content. Oral administration of the extracts to mice and rats showed no signs or symptoms of toxicity in any case. The extracts of A. millefolium, R. officinalis, and M. chamomilla significantly inhibited intestinal transit (p < 0.01 and p < 0.05) when compared to the castor oil control group, with a percentage of intestinal transit similar to that of the reference antidiarrheal loperamide drug (47.8 ± 3.6%). These non-toxic plant extracts constitute a valuable basis to produce dietary supplements for intestinal motility disorders.
Ex-PRESS implantation versus trabeculectomy for long-term maintenance of low intraocular pressure in open angle glaucoma patients
Authors: Tokumo Kana , Okada Naoki , Onoe Hiromitsu , Komatsu Kaori , Masuda Shun , Okumichi Hideaki , Hirooka Kazuyuki , Asaoka Ryo , Kiuchi Yoshiaki 
Published: October 2022

Abstract
Purpose
To compare the efficacy of Ex-PRESS implantation (EXP) to trabeculectomy (TLE) with mitomycin C, for maintaining low target intraocular pressure (IOP) in patients with open-angle glaucoma.
Methods
Patients were randomly assigned to receive EXP or TLE. Surgical success was defined according to three target mean IOP ranges (5 mmHg ≤ IOP ≤ 18 mmHg [criterion A], 5 mmHg ≤ IOP ≤ 15 mmHg [criterion B], and 5 mmHg ≤ IOP ≤ 12 mmHg [criterion C]) representing reductions of at least 20% below baseline on two consecutive follow-up visits 3 months post-surgery, with or without antiglaucoma medication and without further glaucoma surgery.
Results
A total of 73 patients, including 30 in the EXP group and 43 in the TLE group, were included in the study. The baseline IOP was 20.4 ± 4.9 mmHg in the EXP group and 21.9 ± 7.9 mmHg in the TLE group. There were no significant differences in baseline ocular or demographic characteristics between the two groups. There was no statistical difference in IOP every 6 months. After the 3-year follow-up, success rates were A) 60.0% and 60.2%, B) 45.7% and 58.1%, and C) 31.5% and 40.5% for the EXP and TLE groups, respectively. A greater number of glaucoma medications before surgery was associated with a higher failure rate in the TLE group but not the EXP group.
Conclusions
Both procedures resulted in similar IOP reductions and success rates for low target IOP. The number of preoperative glaucoma medications was a risk factor for TLE failure.
Sex differences in the association between the effect of bariatric surgery and cancer risk: A meta-analysis including 18 million individuals
Authors: Li Ya-Dong , Huang hao , Gou Yuan-Qing , Liu Chuan 
Published: October 2022

Abstract
Background
Studies have suggested an association between bariatric surgery for obesity with cancer risk. Whether the effect of bariatric surgery could reduce the same excess risk of overall cancer in women and men is unknown.
Methods
PubMed, EMBASE, and Web of Science were searched to identify cohort studies up to 15 September 2022. Included studies reported the association between bariatric surgery for obesity and cancer risk in men and/or female. Random-effects meta-analyses weighted by the inverse of the variances were applied to pool sex-specific RRs and women-to-men ratios of RRs (RRRs) for overall cancer risk.
Results
18 studies involved with 18,603,812 patients (18,028,898 controls and 573,914 bariatric patients) met the inclusion criteria and were eligible for analysis. The results showed that bariatric surgery was significantly associated with decreased overall cancer risk (RR = 0.68, 95% CI 0.60, 0.78; P < 0.001) in women, but not associated with overall cancer risk (RR = 0.94, 95% CI 0.79, 1.12; P = 0.470) in men. The pooled women-to-men RRR showed an 18% lower reduction of overall cancer associated with bariatric surgery in women than in men (RRR = 0.82, 95% CI 0.75–0.90; P < 0.001). The pooled women-to-men RRR showed an 18% lower reduction of overall cancer associated with bariatric surgery in women than in men RRR = 0.82, 95% CI 0.74–0.92; P < 0.001).
Conclusion
The effect of bariatric surgery is significantly associated with a lower reduction of cancer for women compared to men.
Criterion-related validity of the International Physical Activity Questionnaire (IPAQ) in gastrointestinal cancer patients receiving outpatient chemotherapy
Authors: Iwakoshi Megumi , Tsuji Tetsuya , Takaishi Hiromasa , Hamamoto Yasuo , Sukawa Yasutaka , Hirata Kenro , Muraoka Kaori , Liu Meigen 
Published: October 2022

Abstract
Purpose
To investigate the criterion-related validity of the International Physical Activity Questionnaire (IPAQ) in gastrointestinal cancer patients undergoing outpatient chemotherapy.
Methods
The subjects were gastrointestinal cancer patients receiving outpatient chemotherapy. The correlations of daily activity energy expenditure (AEE) according to the IPAQ-Short-Form(IPAQ-SF), daily AEE with an activity monitor, and energy expenditure from walking were analyzed.
Results
The subjects were 50 people with a mean age of 65.6±10.5 years. The median daily AEE was 86.0 kcal with the IPAQ-SF and 374.0 kcal with the activity monitor, with no correlation between the two. The median daily energy expenditure from walking was 84.3 kcal with the IPAQ-SF and 82.3 kcal with the activity monitor, with a significant correlation (r=0.378) between the two. With a one-week chemotherapy administration interval, significant correlations were seen with daily AEE (r=0.766) and energy expenditure from walking (r=0.888), but no significant correlations were seen with intervals of two weeks or longer.
Conclusions
Using the IPAQ-SF in measurements of energy expenditure from walking during chemotherapy was found to be valid. With respect to AEE, however, when using the IPAQ-SF, it is necessary to evaluate the amount of activity for the most recent week, not a usual week.
Implications for cancer survivors
This study verified the validity of the IPAQ-SF as a screening tool to simply assess physical activity (PA) during outpatient chemotherapy. Implementation of evaluations of PA may facilitate the maintenance of daily exercise habits and assure PA, and improve the continuation of therapy and prolong survival.
Intraoperative bacterial contamination in operations for craniotomy
Authors: Shen Yuan , Dong Chunli , Wang Ruizhi 
Published: October 2022

AbstractBackground:Intraoperative wound sterility in neurosurgical patients were rarely reported. This study used swab samples to prospectively assess the surgical field sterility of craniotomy.Methods:Forty patients admitted for craniotomy were included for surgical field bacterial contamination study during 15 months period. The specimens for bacterial culture were from the skin before cleansing (craniotomy specimen (CS) 1), (CS2) the skin after antiseptic cleansing but before dressing, (CS3) skull immediately after exposure, (CS4) dura immediately after exposure, (CS5) dura immediately before wound closure, (CS6) skull immediately before wound closure, and (CS7) uncovered blood agar plates placed near the instrument table at the marginal laminar air flow (LAF) area for 30 minutes after dura exposure. The culture was conducted on blood agar plates for 5 days to allow for bacterial growth.Results:The culture was positive in CS1 in 34 patients (85.0%), CS5 in 1 patients (2.5%), CS6 in 1 patient (2.5%), and CS7 in 8 patients (20%). No bacterial growth was observed in all CS2-CS4. The frequency of positive cultures in CS5 is significantly higher than that in CS5 and CS6 (P=0.007). The detected bacterial species were indigenous bacteria of the skin.Conclusions:Our study demonstrated low rate of wound contamination during craniotomy. Cultures were more frequently positive in specimens obtained in the marginal LAF area than those in the surgical field.
Knowledge, attitude, and practice of human immune-deficiency virus post exposure prophylaxis among health care workers of governmental hospitals in Addis Ababa, Ethiopia Authors’ detail and affiliation
Authors: Abebe Asmamaw , Adal Ousman , Ayele Teshome 
Published: October 2022

Abstract
Objectives
The main objective of this study will be to assess the knowledge, attitude, and practice of human immune deficiency virus post-exposure prophylaxis for health care workers in government hospitals in the city of Addis Ababa.
Methods
A cross-sectional descriptive study conducted from March to April 2022. The lottery method of simple random sampling technique used to select six government hospitals. Structured self-administered questionnaires used to collect data, which coded to Microsoft Excel and then exported to SPSS Version 26 for descriptive analysis.
Results
large proportion of respondents, 51.6%, found to have poor knowledge about post exposure prophylaxis for human immune deficiency virus. However, most respondents (54.2%) had a favorable attitude toward post-exposure prophylaxis for human immunodeficiency virus, while a significant number of respondents (63.6%) had poor practice.
Conclusion
A significant proportion of respondents had poor knowledge and practice towards post exposure prophylaxis for human immune deficiency virus, even though they had relatively favorable attitudes. Most health care workers (68.9%) did not receive any post-exposure prophylaxis training.
Assessment of Weight Status, Dietary Habits, Physical Activity, and Nutritional Knowledge Among Syrian University Students
Authors: Alazrak Sara , Armashi Humam , Fallouh Deema , Akashe Nawal , Kadri Saeed , Koudsi Abir 
Published: October 2022

Abstract
Introduction: University students are prone to suboptimal health and eating habits. Satisfactory knowledge about healthy food choices is essential for the adoption of behaviors necessary to lead a healthy lifestyle. A web-based cross-sectional study of Syrian university students was performed to assess their knowledge.
Methods: A self-administered dietary questionnaire was published online in Arabic. It included questions about demographic, dietary, lifestyle, health, fitness, hygiene, and knowledge characteristics.
Results: Only 17% of the sample have satisfactory eating habits; 55% of participants have a sedentary life style. 69%; of the participants had a good understanding of healthy and unhealthy eating habits and food; 75% of the participants indicated that they are able to use advice aimed at improving their health, 73% of the participants do not have barriers to change, and about 74% of the participants have good nutritional knowledge; 61% have good food safety knowledge, 33% of the participants have very good hygiene practices.
Conclusion: This study provided preliminary data on various factors that can affect students' health. We found that the BMI of most participating students is within the normal range. As for physical activity; males exercised more than females, and our study showed that about half of the students had a sedentary lifestyle with a higher percentage of females, and males also had better eating habits than females. As for nutritional knowledge, most of the participating male and female students had good nutritional knowledge.
Traffic speed prediction based on spatio-temporal attention convolutional neural network
Authors: Shen Eric Z. 
Published: October 2022

Abstract
Traffic speed is an important index to measure the traffic status, and real-time and accurate traffic speed prediction is an important part of building an intelligent transportation system. A new traffic speed prediction model based on the combination of attention mechanism and graph convolutional neural network is proposed to address the problems of randomness, nonlinearity and spatio-temporal correlation of traffic speed. Finally, the proposed model is combined with five other benchmark models to predict traffic speed on two publicly available traffic speed datasets. The experimental results show that the accuracy of the proposed model is 75.1% and 86.6% on the two datasets, which is about 3% higher than the accuracy of the advanced benchmark model. This indicates that the proposed model has high accuracy and stability, and can provide scientific basis for traffic management.
Wogonin Restrains the Malignant Progression of Lung Cancer Through
Modulating MMP1 and PI3K/AKT Signaling Pathway
Authors: Zhao Zhi , Guo Junhao , Jin Gang , Hu Yanjing , Nan Fangyuan , Hu Xin , Hu Yunsheng , Han Qun 
Published: October 2022

Background:
Wogonin, a natural flavonoid compound, represses cancer cell growth and
induces cancer cell apoptosis in diverse malignancies. However, the function of Wogonin in lung
cancer cells and its regulatory mechanism deserve to be identified.

Methods:
A549 and H460 cells were treated with Wogonin, and the cell growth, apoptosis, migration
and invasion were measured by CCK-8 and EdU, flow cytometry and Transwell assays. The targeted
genes of Wogonin and lung cancer were identified from the TCMSP and Genecards databases,
respectively. The STRING database and Cytoscape software were used to establish a PPI network and
screen hub genes. GO and KEGG analysis was conducted to explore the functions and signal pathways
related to the hub genes. MMP1 expression in lung cancer was analyzed using the UALCAN
databases, and GSEA was performed utilizing LinkedOmics. Gelatin zymography assay was used to
detect MMP1 activity. MMP1 mRNA expression was detected by qRT-PCR. Besides, MMP1, p-AKT
and c-Myc protein were detected by Western Blot assay.

Results:
Wogonin could suppress the proliferation, migration and invasion of A549 and H460 cells and
induce apoptosis. GO and KEGG enrichment analysis revealed the hub genes were mostly enriched in
re-entry into the mitotic cell cycle and apoptosis. The expression of MMP1 was markedly upregulated
in lung squamous cell carcinoma, lung adenocarcinoma tissues, and lung cancer cell lines. Wogonin
could significantly inhibit MMP1 expression and activity, and overexpression of MMP1 significantly
reversed the effect of Wogonin on the malignant phenotypes of A549 and H460 cells. Wogonin
inhibited the expression of p-AKT and c-Myc protein by regulating MMP1.

Conclusion:
Wogonin can repress lung cancer cells' growth and metastatic potential and promote cell
apoptosis via repressing MMP1 expression and modulating PI3K/AKT signaling pathway.
Empagliflozin Exhibits Hepatoprotective Effects Against Bile Duct Ligation-induced
Liver Injury in Rats: A Combined Molecular Docking Approach to In Vivo Studies
Authors: Shakerinasab Nasrin , Azizi Mahdokht , Mansourian Mahboubeh , Sadeghi Hossein , Salaminia Shirvan , Abbasi Reza , Shahaboddin Mohammad Esmaeil , Doustimotlagh Amir Hossein 
Published: October 2022

Background:
Cholestatic liver damage is a chronic disease caused by dysfunction of the hepatic-biliary system. Oxidative stress and inflammation are essential factors in the pathogenesis of cholestasis. Thus, the current study was designed to examine the effect of empagliflozin on bile duct ligation-induced liver damage in rats.

Materials and methods:
This study was done on male Wistar rats, which were randomly assigned to the four experimental groups: sham control (SC), bile duct ligation (BDL), SC plus empagliflozin (SC+EMPA) (Receive 10 mg of EMPA orally for 7 days), BDL plus empagliflozin 10 mg/kg (BDL+ EMPA). At the end of the study, the rats were sacrificed, and serum and tissue samples were collected to analyze biochemical parameters, biomarkers of oxidative stress, inflammatory markers, and histopathological changes. The molecular docking technique was performed to elucidate the interaction of EMPA and Cu/Zn-superoxide dismutase (SOD1).

Results:
The results showed that BDL elevated the serum activity of ALT, AST, ALP, and levels of TBIL and TPro. BDL also intensifies the oxidative stress state in rats, which was confirmed by augmenting lipid peroxidation (MDA), protein oxidation (PCO), and altering antioxidant defense parameters through decrease SOD, catalase (CAT), and glutathione peroxidase (GPX). Furthermore, the histopathological changes in the liver demonstrate the aggravation of inflammation and oxidative stress. In contrast, treatment with EMPA has shown anti-inflammatory and anti-oxidant effects by reducing TNF-a and IL-6 pro-inflammatory marker proteins, restoring the antioxidant status (increased SOD and GPX), reducing ALT, AST, ALP, TBIL, and protein oxidation, and improving the histopathological alterations through reducing bile duct proliferation, fibrosis, focal and portal inflammation. According to the attained findings, the SOD1 activity can be regulated by the EMPA. Our documentation presents direct evidence at the molecular level related to the ability of EMPA to exert its antioxidant performance through certain measures in a particular molecular route.

Conclusions:
The results showed EMPA had hepatic protective effects in rats against cholestatic liver injury, an effect mediated by its antioxidant and anti-inflammatory properties.
Chronic Non-bacterial Osteomyelitis (CNO) In Childhood: A Review
Authors: Price-Kuehne Fiona , Armon Kate 
Published: October 2022

Abstract:
Chronic non-bacterial osteomyelitis (CNO) is an autoinflammatory bone disorder mostly
affecting children and adolescents. Although it is considered a rare disease, CNO is likely to be the
single most common autoinflammatory bone disease in childhood, underdiagnosed and underreported
due to a lack of awareness of the condition in both medics and patients and the absence of
validated diagnostic criteria. The exact underlying pathogenesis of CNO remains unknown, making
targeted treatment difficult. This issue is exacerbated by the lack of any randomised control trials,
meaning that treatment strategies are based solely on retrospective reviews and case series.

:
This review summarises the current concepts in pathophysiology, the clinical features that help differentiate
important differential diagnoses, and an approach to investigating and managing children
with CNO. Ultimately, the timely and thorough investigation of children and young people with
CNO is vitally important to exclude important mimics and initiate appropriate management that can
prevent the complications of persistent inflammatory bone disease.
Oral Hygiene Instruction – How it Affects the Caries Experience, Oral Hygiene Status, and sCD14 Levels?
Authors: Aripin Dudi , Suwargiani Anne Agustina , Wardani Riana , Susilawati Sri 
Published: October 2022

Background:
Caries experience, oral hygiene status, and salivary sCD14 level are three essential factors to analyse individual dental caries development risk. Therefore, regular and effective oral hygiene instruction (OHI) is essential for dental caries prevention.

Objective:
This study aims to analyse the differences in caries experience, oral hygiene status, and sCD14 levels before and after OHI.

Methods:
An observational study with intervention before and after oral hygiene instruction was conducted on 40 samples of each group taken with an accidental sampling method. The sample was calculated using the sample size formula to determine the difference between two paired means; confidence level 95%; power test 90%. The OHI materials include microbial aspects, plaque control methods and instruments, and directives for visiting the dentist. Caries' experience was obtained using DMF-T index; oral hygiene status using simplified oral hygiene instruction (OHI-S); sCD14 levels obtained from unstimulated saliva using an ELISA kit. Data analysis used the Kolmogorov-Smirnov normality test, paired t-test, and Wilcoxon signed-rank test.

Results:
Kolmogorov normality test showed that the caries experience data were normally distributed, while oral hygiene status and sCD14 were not. The paired-t difference test result for caries experience cannot be determined because the data showed a similar value. The Wilcoxon signed-rank test result for oral hygiene status showed no significant difference, while the sCD14 showed a significant difference.

Conclusion:
Oral hygiene instruction showed a similar value of caries experience before and after oral hygiene, and also no difference was found in oral hygiene status; however, the sCD14 levels showed a difference after oral hygiene instruction.