bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the efficacy of manual soft tissue therapy and therapeutic exercises in patients with pain and limited mobility TMJ: a randomized control trial (RCT).
Authors: Gębska Magdalena , Dalewski Bartosz , Pałka Łukasz , Kołodziej Łukasz 
Published: October 2022

Abstract
Summary
The limited number of randomized controlled trials (RCTs) comparing the efficacy of soft tissue manual therapy and self-therapy interventions prompted the authors to focus on the analgesic and myorelaxant use of massage, post-isometric muscle relaxation (PIR) and therapeutic exercise in TMD patients.
Objectives
To evaluate the effectiveness of soft tissue therapy and therapeutic exercises in female patients with pain, increased masseter muscle tension and limited mandibular mobility.
Material and Methods
The study was conducted on a group of 82 women (G1) with the Ib disorder diagnosed in DC/TMD (Ib - myofascial pain with restricted mobility). The control group (G2) consisted of 104 women without diagnosed TMDs (normal reference values for TMJ ROM and masseter muscle sEMG bioelectric activity). Diagnostic procedures were performed in both groups (sEMG of the masseter muscles at baseline and during exercise, measurement of TMJ mobility, assessment of pain intensity - NRS scale). The G1 group was randomly divided into 3 therapeutic groups in which the therapy was carried out for 10 days: therapeutic exercises (TE), manual therapy – massage and therapeutic exercises (MTM_TE), manual therapy – PIR and therapeutic exercises (MTPIR_TE). Each time after therapy, the intensity of pain and TMJ mobility were assessed. Sealed, opaque envelopes were used for randomization. After 5 and 10 days of therapy, bilateral sEMG signals of the masseter muscles were acquired.
Results
Massage, PIR and self-therapy led to a decrease in sEMG at rest as well as in exercise. After day 6 of therapy, the groups obtained a significant difference (p = 0.0001). Each of the proposed forms of therapy showed a minimal clinically significant difference (MID) in the sEMG parameter at the endpoint, with the most considerable difference in the MTM_TE group. The forms of MT used were effective in reducing the patients' pain intensity; however, a significant difference between therapies occurred after 4 treatments (p = 0.0001). Analyzing the MID between methods, it was observed that self-therapy had an analgesic effect only after 8 treatments, while PIR after 3 and massage after 1 treatment. After day 7, the mean pain score in the MTM_TE group was 0.889 and in the TMPIR_TE group was 3.44 on the NRS scale. In terms of MMO, a significant difference was obtained between monotherapy and each form of TM, i.e. massage (p = 0.0001) and PIR (p = 0.0001). Analyzing mandibular lateral movements, the authors got a significant difference in the proposed MT forms, of which massage treatments exceeded the effectiveness of PIR.
Conclusions
Soft tissue manual therapy and therapeutic exercise are simple and safe interventions that can potentially benefit patients with myogenic TMDs, with massage showing better analgesic effects than PIR.
LncRNA LLNLR-299G3.1 promotes ESCC progression by regulating cancer related genes through RNA-chromatin interactions
Authors: Tian Li , Huang Yongyi , Zhang Baozhen , Song Yi , Yang Lin , Chen Qianqian , Wang Zheng , Wang Yiling , He Qihan , Yang Wenhan , Yu Shuyong , Lu Tianyu , Gao Kaiping , Fan Xiujun , Song Jian , Zhai Rihong 
Published: October 2022

Abstract
Background
Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of cancers, including esophageal squamous cell carcinoma (ESCC). However, the mechanisms of lncRNAs in ESCC are still largely unknown and therapeutic attempts for in vivo targeting disease-associated lncRNA remain a challenge.
Methods
By RNA-sequencing analysis, we identified that LNLR-299G3.1 was a novel ESCC-associated lncRNA. Expression level of LLNLR-299G3.1 was determined by qRT-PCR and RACE. The functional significance of LLNLR-299G3.1 was assessed by knockdown or over-expression. plCSA-BP (placental chondroitin sulfate A binding peptide)-coated nanoparticles (NPs) were developed for targeting delivery of antisense oligonucleotide (ASO)-LLNLR-299G3.1 in vivo. RNA pull-down, mass spectrometry, RNA-seq, ChIRP-seq (chromatin isolation by RNA purification and sequencing), qRT-PCR assays, and rescue assays were performed to explore the mechanisms of LLNLR-299G3.1 in ESCC.
Results
LNLR-299G3.1 was up-regulated in ESCC tissues and cells and promoted ESCC cell proliferation and invasion. Silencing of LLNLR-299G3.1 with ASO resulted in opposite effects. Moreover, intravenous delivery of pICSA-NP loaded ASO (pICSA-ANPs) strongly inhibited ESCC tumor growth and significantly improved animal survival in vivo. Mechanistically, LLNLR-299G3.1 bound to cancer-associated binding proteins and regulated the expression of cancer-related genes, including OSM, TNFRSF4, HRH3, and SSTR3. ChIRP-seq revealed that these genes contained enriched chromatin binding sites for LLNLR-299G3.1. Rescue experiments confirmed that the effects of LLNLR-299G3.1 on ESCC cell proliferation were dependent on interaction with HRH3 and TNFRSF4.
Conclusion
LLNLR-299G3.1 was a novel oncogenic lncRNA that promoted ESCC malignancy through gene-chromatin interactions. In vivo targeting ESCC by pICSA-BP-ANPs may be an effective strategy for the treatment of lncRNA-associated ESCC.
The Metastable Associations of Bacteriophages and Erwinia amylovora
Authors: Besarab Natalya V , Letarova Maria , Babenko Vladislav , Belalov Ilya , Golomidova Alla , Kulikov Eugene , Lagonenko Alexander , Evtushenkov Anatoly , Letarov Andrey 
Published: October 2022

Abstract
Cultivation of bacteria and phages on solid media can serve as a model for studying the interaction of phage and bacterial population under the diffusion-limited conditions, which frequently take place in nature. Here we describe bacteriophages VyarbaL and Hena2, the members of the Molineuxvirinae and the Ounavirinae subfamilies, respectively, that are able to form the pseudolysogenic associations (PA) with their host E. amylovora 1/79Sm on solid media. These PAa were stable through multiple passages. The phenomenon of the PA formation between a bacterial culture and bacteriophages affect the effectiveness of preparations based on virulent bacteriophages.
The stability of dams with different stoping elevations in Tongling valley type tailings impoundment: A case study in Yunnan China
Authors: Wang Kai , Zuo Xiaohuan , Du Feng , Yang Yi , Xu Chao 
Published: October 2022

Abstract
Significant interest has been focused on recovery rates, recovery options, and recovery utilization when tailings impoundments are re-mined. However, the stability of the tailings dams during the recovery process is also a severe issue. Based on engineering geological survey and laboratory tests, the Tongling tailings impoundment’s characteristics of instability evolution under different recovery heights and diverse working conditions were analyzed by numerical simulation. Firstly, with the help of 2D software, the position of the tailings dam infiltration line and the alteration of the dam safety factor during the stoping process were calculated. Secondly, 3Dmine software was used to create the 3D surface structure of the tailings impoundment, and then a 3D numerical analysis model was established by means of Midas GTS NX software. The numerical simulation of seepage and stress analyses were conducted based on the model. Consequently, the evolution of the stability characteristics of tailings dam under different operating conditions was calculated. The research demonstrates that the dry beach length of the tailings pond gradually reduces with the decrease of the extraction height, resulting in a lower infiltration line. Under flood conditions, the saturation line has partial overflow due to the poor seepage discharge capacity of the dam. The total displacement of the dam body is inversely proportional to the retrieval height. The more extreme the analyzed working conditions, the more the safety factor will be reduced. Additionally, the plastic variation area of the dam body will be more comprehensive, which will increase the risk of a dam collapse.
An insight into the development of triple-negative breast cancer at transcriptome level based on single-cell sequencing technology
Authors: Jin Boxiang , Zhu Zhe , Ma Junfeng 
Published: October 2022

Abstract
Poor prognosis and low survival rate always hinder the research on the mechanism and pathology of triple-negative breast cancer (TNBC). With the rapid development of sequencing technology, the sequencing data of TNBC are becoming more widely available. This study attempted to reveal the potential biology of TNBC at both transcriptome and single-cell sequencing levels. Alteration in the differentially expression genes of TNBC was uncovered at the transcriptome level through the dataset GSE62931 and the cell ratio changes were displayed at the single-cell level through six samples from the dataset GSE161529. TNBC was identified at both transcriptome and single-cell levels. Compared with the non-TNBC group, 475 differentially expressed genes were obtained at the transcriptome level in the TNBC group. The differentially expressed genes were mainly enriched in microtubule binding, chromosome segregation, and response to xenobiotic stimulus, as well as pathways in cancer, Tyrosine metabolism, and Mucin type O-glycan biosynthesis. A sub-module with high correlation was further identified after screening of TNBC-related DEG. Compared with the non-TNBC group, the proportions of natural killer T cells, luminal epithelial cells, B cells, and basal cells in the TNBC group were significantly decreased at the single-cell level, but the proportions of T cells, monocytes, and neural progenitor cells significantly increased. The transcriptome results could be combined with the single-cell sequencing results through the submodule, based on which we studied the key genes related to the prognosis of TNBC patients, including RRM2, TPX2, CENPF and TOP2A. We found that the expression of these key genes at the single-cell level was heterogeneous with that at the overall cellular level. To conclude, the expression of RRM2, TPX2, CENPF, and TOP2A is heterogeneous at the cellular level, inconsistent with the expression of TNBC at the overall cellular level. Therefore, it is necessary to combine the changes at the gene level and the cellular level for research.
High Dose of Metformin Decreases Susceptibility to Occlusive Arterial Thrombosis in Type-2 Diabetic Mice
Authors: Alvidrez Roberto Ivan Mota , Annarapu Gowtham K. , Sriniv Amudan J. , Liu Zeyu , Yazdani Hamza O. , Nolfi-Donegan Deidre , Simmons Richard L. , Shiva Sruti , Neal Matthew D. 
Published: October 2022

Abstract

Introduction Metformin is the most prescribed medication in Type 2 Diabetes(T2D). Metformin has shown to decrease mean platelet volume, with promising antiplatelet effects. High doses of Metformin have also been associated with hypercoagulation. We hypothesize that Metformin will protect T2D mice from occlusive arterial thrombus formation by altering platelet activation and mitochondrial bioenergetics. Methods T2D was developed by low dose of Streptozotocin, non-T2D (healthy) mice are controls. Either vehicle or Metformin was administered twice daily via oral gavage for 7-days. Ferric chloride (FeCl3) arterial thrombosis and tail bleeding time were performed. Whole blood aggregometry, platelet activation/adhesion and mitochondrial bioenergetics were evaluated. Results Metformin decreased susceptibility of T2D mice to arterial thrombosis. Platelet bioenergetics show T2D mice have increased platelet mitochondrial respiration, but no differences were observed with Metformin treatment. In non-T2D mice, Metformin modulated ADP-dependent increase in platelet adhesion. In non-T2D mice, Metformin shortens bleeding time with faster thrombotic occlusion. Metformin also increased platelet mitochondrial maximal respiration and spare respiratory capacity uniquely in non-T2D mice. Conclusion Metformin regulates platelet bioenergetics and ADP-mediated platelet function in T2D mice which attenuates susceptibility to arterial thrombosis. Future studies will evaluate clinically relevant doses of Metformin that regulates thrombotic function in diabetic platelets.
Single-index models for extreme value index regression
Authors: Yoshida Takuma 
Published: October 2022

Abstract
Since the extreme value index (EVI) controls the tail behaviour of the distribution function, the estimation of EVI is a very important topic in extreme value theory. Recent developments in the estimation of EVI along with covariates have been in the context of nonparametric regression. However, for the large dimension of covariates, the fully nonparametric estimator faces the problem of the curse of dimensionality. To avoid this, we apply the single index model to EVI regression under Pareto-type tailed distribution. We study the penalized maximum likelihood estimation of the single index model. The asymptotic properties of the estimator are also developed. Numerical studies are presented to show the efficiency of the proposed model.
MSC Classification: 62G08 , 62G20 , 62G32
Historical and regional particularities in the prevalence of traumatic events and posttraumatic stress disorder in Germany
Authors: Kasinger Christoph , Schulz Ann-Christin , Glaesmer Heide , Ulke Christine , Maercker Andreas , Beutel Manfred , Brähler Elmar 
Published: October 2022

Abstract
Purpose
Research on prevalence rates of traumatic events and PTSD has shown significant differences between countries, due to their different history and socialization processes. In the case of Germany, with its history of two divided states, this is highly relevant. This study explores the prevalence of traumatic events and PTSD in formerly divided East and West Germany.
Methods
We used data of four representative surveys (years 2005, 2007, 2008 and 2016) for the prevalence of traumatic events with a total of N = 9.200 respondents. For the analyses of the prevalence of PTSD we used data of three representative surveys (years 2005, 2007, 2008) with a total of N = 6676 respondents. We compared different birth cohorts as well as persons who lived in the former West vs. East using a Chi-Square-Test.
Results
We found significant differences in the occurrence of traumatic events between genders, different age cohorts as well as between people who live in East and West Germany. Significant differences in the prevalence of PTSD can only be observed for different age cohorts. Most of the age effects may be due to traumatic events related to WWII.
Conclusions
Our data suggests that socio-political factors may need to be considered when accounting for differences in occurrence rates of traumatic events between East and West Germany. However, we couldn’t find any differences in the overall prevalence of PTSD between the former GDR and FRG. Nevertheless, future epidemiological trauma research should take historical and regional peculiarities of countries into account.
A 90 nm Active Inductor Feedback Transimpedance Amplifier For Fiber Optics Applications
Authors: Alsheikhjader Muhammed Subhi Hameed 
Published: October 2022

Abstract
A simulated 90 nm active inductor feedback transimpedance amplifier is reported. A 1V configuration of regulated cascode followed by a shunt-shunt feedback stage is considered with a local active inductor feedback configuration. A transimpedance gain of 62.9 dBΩ is achieved with a bandwidth of 2.7 GHz, power consumption of 1.22 mW and an input referred noise current spectral density of 21 pA/√Hz. The key advantage of using local active inductor feedback is to solve a major problem regarding integrated circuit design constraint in which it replaces an ordinary spiral inductor and therefore, real advantages of less volume on board a chip, low power consumption and input referred noise are obtained. Important transient analysis and Fourier transform analysis were performed to examine active inductor feedback system response. There was no apparent inductive peaking associated with transimpedance gain despite of the fact that the frequency response of active inductor impedance behavior was to a greater extent similar to that of an ordinary spiral inductor.
A graph-based deep learning approach for illegal transaction detection in Bitcoin.
Authors: Heidari Alireza , Bahrak Behnam 
Published: October 2022

Abstract
In recent years, cryptocurrencies have been used as a new way to conduct transactions and transfer money among individuals, so the volume of daily transactions in their networks has reached to several billion dollars. The anonymity of users alongside the high security and privacy properties has led many criminals to the cryptocurrency networks to carry out their illegal transactions. However, public access to the blockchain of Bitcoin and many other cryptocurrencies, allows individuals and financial institutions to obtain information about some of these activities. Several approaches, such as investigating financial flow in the blockchain, statistical analysis, and machine learning methods, have been introduced to detect illegal transactions. This paper uses a deep learning model based on a graph convolutional network and multi-layer perceptron to classify Bitcoin transactions based on their applications. We extract several features from the transaction graph, then by doing some preprocessing on our data, we train a model, which is able to predict illicit transactions with an f1-score of 97.09% which outperforms previous approaches to this problem.
Salmonella typhimurium induces intestinal inflammatory response through activation of Cav- 1/Akt/IKKα/NF-κB signaling pathway
Authors: Li Haihua , Shang Zhiyuan , Wei Zhaoyang , Wang Yimeng , Liu Xuejiao , Qiao Jiayun 
Published: October 2022

Abstract
Salmonella, a pathogenic gram-negative bacterium, has been reported to cause intestinal inflammatory response, which is found in both humans and animals. However, the underlying mechanism is not quite clear. To this end, we used Salmonella typhimurium (ST)-infected mice or intestinal epithelioid cell line 6 (IEC-6 cells) as a model to explore the roles of ST in reducing inflammatory response and elucidate the potential mechanisms. In in vivo experiments, mice were given ST with different concentrations (2.5×106 cfu/mL, 2.5×107 cfu/mL and 2.5×108 cfu/mL). The inflammatory markers in the serum were measured and the changes of small intestinal structures were observed. Differentially expressed proteins in the jejunum were screened using TMT-based quantitative proteomic analysis, and verified using real-time quantitative PCR (qPCR) and western blotting. In in vitro experiments, IEC-6 cells were pretreated with particular inhibitor for 24 h and then infected with ST (1×103 cfu/mL) for 12 h. The lactic dehydrogenase (LDH), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) levels in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA) while the protein expression of caveolin-1 (Cav-1), protein kinase B (Akt), inhibitor kappa B kinaseα (IKKα) and nuclear factor-kappaB (NF-κB) p65 were measured by western blotting. ST promoted the production of serum inflammatory markers and the disruption of duodenal and jejunal structures in a dose-dependent manner over a specific period of time. Proteomic analysis showed that ST-induced intestinal inflammatory response was associated with the expression of Cav, Akt, IKK and NF-κB. In addition, we further confirmed that the activation of Cav-1/Akt/IKKα/NF-κB signaling pathway played a critical role in ST infection prompting the production of inflammatory markers and causing inflammatory response in IEC-6 cells. Taken together, our finding revealed that ST could induce intestinal inflammatory response through activating the Cav-1/Akt/IKKα/NF-κB signaling pathway.
T2 gallbladder carcinoma: Clinicopathological features and optimal surgical strategies
Authors: Endo Yuichi , Amano Shota , Orimoto Hiroki , Kawamura Masahiro , Fujinaga Atsuro , Kawano Yoko , Kawasaki Takahide , Masuda Takashi , Hirashita Teijiro , Ohta Masayuki , Inomata Masafumi 
Published: October 2022

Abstract
Purpose: For gallbladder carcinomas (GBCs), the only effective treatment is surgery. In this study, we aimed to determine the clinicopathologicalfeatures of T2 GBC and identify the optimal surgical strategies.
Methods: This was a retrospectivestudy of 77 patients with histologically verified T2 subserosal GBC who underwent surgery at our institution between 1989 and 2021. Pathological data (tumor size, location, grade, lymph node status, and lymphovascular and perineural invasions) and demographic data were reviewed. The 5-year disease-specific survival (DSS) rates were determined and included in our analysis.
Results: The 42 (55%) patients in our sample who were pathological invasion (PI)-positive had significantly lower 5-year DSS. PI was significantly correlated with lymph node metastasis but not with tumor location. Those with PI treated with radical cholecystectomies had better 5-year DSS rates than those treated with simple cholecystectomies (57% vs 25%, p = 0.04). However, different surgical procedures produced no significant differences in the 5-year DSS of PI-negative patients.
Conclusions: PI-positive T2 GBC patients had poor prognoses and required radical cholecystectomy. However, PI-negative cases had low lymph node metastases rates and favorable prognoses. In these cases, simple cholecystectomy may be more appropriate.
Safety of oral propranolol for neonates with problematic infantile hemangioma: a study in an Asian population
Authors: Fu Ronghua , Zou Yun , Wu Zhiping , Jin Pingliang , Cheng Jun , Bai Hanxiang , Huang Mengyu , Huan Xiangquan , Yuan Hua 
Published: October 2022

Abstract
Purpose: The overriding goal of this study was to evaluate the safety of initiating and maintaining propranolol for problematic infantile hemangioma (IH) in neonates.
Methods: Clinical data of 112 infants with IH admitted from January 2016 to December 2020 Department of Plastic Surgery, Jiangxi Provincial Children's Hospital were analyzed retrospectively. All of patients were evaluated during the hospital at the beginning of the treatment and later in outpatient settings during the treatment. Each time, the following monitoring methods were applied: physical examination, ultrasound echocardiography (UCG), electrocardiography (ECG), blood pressure (BP), heart rate (HR), and basic laboratory tests included blood glucose (BG), liver function, blood potassium, thyroid function.
Results: There was a significant reduction in the BP and HR at the initiation of treatment. The incidences of bradycardia and hypoglycemia were observed to be increased with the prolong duration of treatment, but not prolonged PR interval. During the course of the therapy, the risk of hyperkalemia and hypothyroidism was reached maximum at the 2 months and 3 months, respectively. Physical growth index including average height, weight and head circumference was not influenced by the treatment. The observed adverse effects were majority mild and only 3 patients needed to rest for 7 days due to severe diarrhea before restarting treatment.
Conclusions: Propranolol is safe and well-tolerated by properly selected young infants with IH. No serious adverse events were observed. Larger, prospective studies are indicated to investigate propranolol in young infants.