bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phytochemical study and immunomodulatory activity of Fraxinus excelsior L.
Authors: Qasaymeh Rana Mohammad , Rotondo Dino , Seidel Veronique 
Published: November 2022

Abstract

Objectives
Fraxinus excelsior L. (FE) is traditionally used to treat inflammatory and pain disorders. This study aimed to identify the constituents of FE leaves and evaluate the effects of its n-hexane (FEH), ethyl acetate (FEE), methanol (FEM) extracts and constituents on the viability of THP-1 cells and their ability to release pro-inflammatory cytokines.

Methods
THP-1 cell viability was assessed using an MTT assay. The immunomodulatory activity was evaluated by measuring tumour necrosis factor-alpha (TNF-α) and interleukin 12 (IL-12) released by lipopolysaccharide-stimulated THP-1 cells using enzyme-linked immunosorbent assays.

Key findings
Triterpenes, tyrosol esters, alkanes, phytyl and steryl esters, pinocembrin and bis(2-ethylhexyl)phthalate were isolated from FE. The tyrosol esters showed no significant effect on THP-1 cell viability. FEH, FEE, FEM, and pinocembrin, ursolic acid, oleanolic acid had IC50 values of 56.9, 39.9, 124.7 µg/ml and 178.6, 61.5 and 199.8 µM, respectively. FE extracts, ursolic acid, oleanolic acid and pinocembrin significantly reduced TNF-α/IL-12 levels. The tyrosol esters did not significantly affect TNF-α/IL-12 production.

Conclusions
FE was able to reduce pro-inflammatory cytokine production indicating a mechanistic focus in its use for inflammation and pain. Further investigations are warranted to unravel the mode of action of the tested constituents and discover other potentially active compounds in FE extracts.
Escherichia coli expressing chloroplast chaperones as a proxy to test heterologous Rubisco production in leaves
Authors: Buck Sally , Rhodes Tim , Gionfriddo Matteo , Skinner Tanya , Yuan Ding , Birch Rosemary , Kapralov Maxim V , Whitney Spencer M 
Published: November 2022

Abstract
Rubisco is a fundamental enzyme in photosynthesis and therefore for life. Efforts to improve plant Rubisco performance have been hindered by the enzymes’ complex chloroplast biogenesis requirements. New Synbio approaches, however, now allow the production of some plant Rubisco isoforms in Escherichia coli. While this enhances opportunities for catalytic improvement, there remain limitations in the utility of the expression system. Here we generate, optimize, and test a robust Golden Gate cloning E. coli expression system incorporating the protein folding machinery of tobacco chloroplasts. By comparing the expression of different plant Rubiscos in both E. coli and plastome-transformed tobacco, we show that the E. coli expression system can accurately predict high level Rubisco production in chloroplasts but poorly forecasts the biogenesis potential of isoforms with impaired production in planta. We reveal that heterologous Rubisco production in E. coli and tobacco plastids poorly correlates with Rubisco large subunit phylogeny. Our findings highlight the need to fully understand the factors governing Rubisco biogenesis if we are to deliver an efficient, low-cost screening tool that can accurately emulate chloroplast expression.
The miniJPAS survey: stellar atmospheric parameters from 56 optical filters
Authors: Yuan H-B , Yang L , Cruz P , Jiménez-Esteban F , Daflon S , Placco V M , Akras S , Alfaro E J , Galarza C Andrés , Gonçalves D R , Duan F-Q , Liu J-F , Laur J , Solano E , Borges Fernandes M , Cenarro A J , Marín-Franch A , Varela J , Ederoclite A , López-Sanjuan Carlos , Abramo R , Alcaniz J , Benítez N , Bonoli S , Cristóbal-Hornillos D , Dupke R A , Hernán-Caballero Antonio , Mendes de Oliveira C , Moles M , Sodré L , Vázquez Ramió Héctor , Taylor K 
Published: November 2022

ABSTRACT
With a unique set of 54 overlapping narrow-band and two broader filters covering the entire optical range, the incoming Javalambre-Physics of the Accelerating Universe Astrophysical Survey (J-PAS) will provide a great opportunity for stellar physics and near-field cosmology. In this work, we use the miniJPAS data in 56 J-PAS filters and 4 complementary SDSS-like filters to explore and prove the potential of the J-PAS filter system in characterizing stars and deriving their atmospheric parameters. We obtain estimates for the effective temperature with a good precision (&lt;150 K) from spectral energy distribution fitting. We have constructed the metallicity-dependent stellar loci in 59 colours for the miniJPAS FGK dwarf stars, after correcting certain systematic errors in flat-fielding. The very blue colours, including uJAVA − r, J0378 − r, J0390 − r, uJPAS − r, show the strongest metallicity dependence, around 0.25 mag dex−1. The sensitivities decrease to about 0.1 mag dex−1 for the J0400 − r, J0410 − r, and J0420 − r colours. The locus fitting residuals show peaks at the J0390, J0430, J0510, and J0520 filters, suggesting that individual elemental abundances such as [Ca/Fe], [C/Fe], and [Mg/Fe] can also be determined from the J-PAS photometry. Via stellar loci, we have achieved a typical metallicity precision of 0.1 dex. The miniJPAS filters also demonstrate strong potential in discriminating dwarfs and giants, particularly the J0520 and J0510 filters. Our results demonstrate the power of the J-PAS filter system in stellar parameter determinations and the huge potential of the coming J-PAS survey in stellar and Galactic studies.
Stark broadening of Fe xxv spectral lines
Authors: Dimitrijević Milan S , Christova Magdalena D , Yubero Cristina , Sahal-Bréchot Sylvie 
Published: November 2022

ABSTRACT
Stark broadening parameters, full widths at half-maximum of intensity and shifts for 18 spectral lines of Fe xxv, broadened by collisions with electrons, protons, and Fe xxvii ions, have been calculated using the impact semiclassical perturbation formalism for a grid of electron densities and temperatures of interest for neutron star atmospheres and their environment.
The Type 1 and Type 2 AGN dichotomy according to their ZTF optical variability
Authors: López-Navas E , Arévalo P , Bernal S , Graham Matthew J , Hernández-García L , Lira P , Sánchez-Sáez P 
Published: November 2022

ABSTRACT
The scarce optical variability studies in spectrally classified Type 2 active galactic nuclei (AGNs) have led to the discovery of anomalous objects that are incompatible with the simplest unified models (UMs). This paper focuses on the exploration of different variability features that allow to distinguish between obscured, Type 2 AGNs and the variable, unobscured Type 1s. We analyse systematically the Zwicky Transient Facility, 2.5-yr-long light curves of ∼15 000 AGNs from the Sloan Digital Sky Survey Data Release 16, which are generally considered Type 2s due to the absence of strong broad emission lines (BELs). Consistent with the expectations from the UM, the variability features are distributed differently for distinct populations, with spectrally classified weak Type 1s showing one order of magnitude larger variances than the Type 2s. We find that the parameters given by the damped random walk model lead to broader H α equivalent width for objects with τg &gt; 16 d and long-term structure function SF∞, g &gt; 0.07 mag. By limiting the variability features, we find that ∼11 per cent of Type 2 sources show evidence for optical variations. A detailed spectral analysis of the most variable sources (∼1 per cent of the Type 2 sample) leads to the discovery of misclassified Type 1s with weak BELs and changing-state candidates. This work presents one of the largest systematic investigations of Type 2 AGN optical variability to date, in preparation for future large photometric surveys.
Perceptions of Death Among Patients with Advanced Cancer Receiving Early Palliative Care and Their Caregivers: Results from a Mixed-Method Analysis
Authors: Bigi Sarah , Ganfi Vittorio , Borelli Eleonora , Potenza Leonardo , Artioli Fabrizio , Eliardo Sonia , Mucciarini Claudia , Cottafavi Luca , Ferrari Umberto , Lombardo Laura , Cagossi Katia , Pietramaggiori Alessandra , Fantuzzi Valeria , Bernardini Ilaria , Cruciani Massimiliano , Cacciari Cristina , Odejide Oreofe , Adolfo Porro Carlo , Zimmermann Camilla , Efficace Fabio , Bruera Eduardo , Luppi Mario , Bandieri Elena 
Published: November 2022

AbstractBackgroundOncologists are often concerned that talking about death with patients may hinder their relationship. However, the views of death held by patients have not been thoroughly investigated. This study aimed to describe the perception of death among patients with advanced cancer receiving early palliative care (EPC) and their caregivers.Material and MethodsQualitative and quantitative analyses were performed on 2 databases: (a) transcripts of open-ended questionnaires administered to 130 cancer patients receiving EPC with a mean age of 68.4 years and to 115 primary caregivers of patients on EPC with a mean age of 56.8; (b) texts collected from an Italian forum, containing instances of web-mediated interactions between patients and their caregivers.ResultsQuantitative analysis shows that: (a) patients and caregivers are not afraid of speaking about death; (b) patients and caregivers on EPC use the word “death” significantly more than patients on standard oncology care (SOC) and their caregivers (P &lt; .0001). For both participants on EPC and SOC, the adjectives and verbs associated with the word “death” have positive connotations; however, these associations are significantly more frequent for participants on EPC (verbs, Ps &lt; .0001; adjectives, Ps &lt; .003). Qualitative analysis reveals that these positive connotations refer to an actual, positive experience of the end of life in the EPC group and a wish or a negated event in the SOC group.ConclusionsEPC interventions, along with proper physician-patient communication, may be associated with an increased acceptance of death in patients with advanced cancer and their caregivers.
The Moral Rights and Wrongs of Online Dating and Hook-Ups
Authors: Frank Lily , Klincewicz Michal 
Published: November 2022

Abstract
In this chapter we identify three potentially morally problematic behaviours that are common among users of dating and hook-up apps (DHAs) and provide arguments as to why they may or may not be considered (a) in a category of their own, distinct from similar behaviours outside of DHAs; (b) caused or facilitated by affordances and business logic of DHAs; (c) as indeed morally wrong. We also consider ways in which morally problematic behaviours can be anticipated, mitigated, or even prevented by analysis of the ethical and moral dimensions of technologies and their afforded uses. Finally, we offer some possible directions for future work on these topics in particular and on the ethical consequences of DHAs in general.
Interpretability and Transparency in Artificial Intelligence
Authors: Mittelstadt Brent 
Published: November 2022

Abstract
Artificial Intelligence (AI) systems are frequently thought of as opaque, meaning their performance or logic is thought to be inaccessible or incomprehensible to human observers. Models can consist of millions of features connected in a complex web of dependent behaviours. Conveying this internal state and dependencies in a humanly comprehensible way is extremely challenging. Explaining the functionality and behaviour of AI systems in a meaningful and useful way to people designing, operating, regulating, or affected by their outputs is a complex technical, philosophical, and ethical project. Despite this complexity, principles citing ‘transparency’ or ‘interpretability’ are commonly found in ethical and regulatory frameworks addressing technology. This chapter provides an overview of these concepts and methods design to explain how AI works. After reviewing key concepts and terminology, two sets of methods are examined: (1) interpretability methods designed to explain and approximate AI functionality and behaviour; and (2) transparency frameworks meant to help assess and provide information about the development, governance, and potential impact of training datasets, models, and specific applications. These methods are analysed in the context of prior work on explanations in the philosophy of science. The chapter closes by introducing a framework of criteria to evaluate the quality and utility of methods in explainable AI (XAI) and to clarify the open challenges facing the field.
Explainable Machine Learning, Patient Autonomy, and Clinical Reasoning
Authors: Keeling Geoff , Nyrup Rune 
Published: November 2022

Abstract
Clinical decision support systems based on complex machine learning models render opaque the rationale and value commitments that underpin diagnoses and suggested treatments. This creates a tension with the prevailing view in medical ethics, which emphasizes patients making autonomous decisions based on an understanding of relevant medical evidence alongside their beliefs and values. Calls for algorithmic explainability in clinical settings are partly motivated by this tension. The question is what needs to be explained, to whom, in what way, and when, to integrate machine learning systems into the clinical process in a way that is consistent with patient-centred decision-making. In this chapter, the authors review the tensions and argue that answers to these questions depend on more fundamental issues in the philosophy of medicine regarding the logic of clinical reasoning. The authors outline and defend a broadly Peircean account which, they argue, captures ethically salient aspects of the interplay between clinicians and decision support systems, and use it to shed light on the particulars of the explainability challenge.
Arrest, Senescence and Death of Shoot Apical Stem Cells inArabidopsis thaliana
Authors: Wang Yukun , Shirakawa Makoto , Ito Toshiro 
Published: November 2022

AbstractShoot stem cells act as the source of the aboveground parts of flowering plants. A precise regulatory basis is required to ensure that plant stem cells show the right status during the stages of proliferation, senescence and cell death. Over the past few decades, the genetic circuits controlling stem cell fate, including the regulatory pathways of establishment, maintenance and differentiation, have been largely revealed. However, the morphological changes and molecular mechanisms of the final stages of stem cells, which are represented by senescence and cell death, have been less studied. The senescence and death of shoot stem cells are under the control of a complex series of pathways that integrate multiple internal and external signals. Given the crucial roles of shoot stem cells in influencing plant longevity and crop yields, researchers have attempted to uncover details of stem cell senescence and death. Recent studies indicate that stem cell activity arrest is controlled by the FRUITFULL–APETALA2 pathway and the plant hormones auxin and cytokinin, while the features of senescent and dead shoot apical stem cells have also been described, with dynamic changes in reactive oxygen species implicated in stem cell death. In this review, we highlight the recent breakthroughs that have enriched our understanding of senescence and cell death processes in plant stem cells.
Brassinosteroids promote etiolated apical structures in darkness by amplifying the ethylene response via the EBF-EIN3/PIF3 circuit
Authors: Wang Jiajun , Sun Ning , Zheng Lidan , Zhang Fangfang , Xiang Mengda , Chen Haodong , Deng Xing Wang , Wei Ning 
Published: November 2022

Abstract
Germinated plants grow in darkness until they emerge above the soil. To help the seedling penetrate the soil, most dicot seedlings develop an etiolated apical structure consisting of an apical hook and folded, unexpanded cotyledons atop a rapidly elongating hypocotyl. Brassinosteroids (BRs) are necessary for etiolated apical development, but their precise role and mechanisms remain unclear. Arabidopsis thaliana SMALL AUXIN UP RNA17 (SAUR17) is an apical-organ-specific regulator that promotes production of an apical hook and closed cotyledons. In darkness, ethylene and BRs stimulate SAUR17 expression by transcription factor complexes containing PHYTOCHROME-INTERACTING FACTORs (PIFs), ETHYLENE INSENSITIVE 3 (EIN3), and its homolog EIN3-LIKE 1 (EIL1), and BRASSINAZOLE RESISTANT1 (BZR1). BZR1 requires EIN3 and PIFs for enhanced DNA-binding and transcriptional activation of the SAUR17 promoter; while EIN3, PIF3, and PIF4 stability depends on BR signaling. BZR1 transcriptionally downregulates EIN3-BINDING F-BOX 1 and 2 (EBF1 and EBF2), which encode ubiquitin ligases mediating EIN3 and PIF3 protein degradation. By modulating the EBF-EIN3/PIF protein-stability circuit, BRs induce EIN3 and PIF3 accumulation, which underlies BR-responsive expression of SAUR17 and HOOKLESS1 and ultimately apical hook development. We suggest that in the etiolated development of apical structures, BRs primarily modulate plant sensitivity to darkness and ethylene.
Pain Severity and Pain Interference in Late Pregnancy: An Analysis of Biopsychosocial Factors Among Women Scheduled for Cesarean Delivery
Authors: Wilson Jenna M , He Jingui , Flowers K Mikayla , Kovacheva Vesela , Soens Mieke , Schreiber Kristin L 
Published: November 2022

Abstract

Objective
Pain is a variably experienced symptom during pregnancy, and women scheduled for cesarean delivery, an increasingly common procedure, are a relatively understudied group who might be at higher pain risk. Although biopsychosocial factors are known to modulate many types of chronic pain, their contribution to late pregnancy pain has not been comprehensively studied. We aimed to identify biopsychosocial factors associated with greater pain severity and interference during the last week of pregnancy.

Methods
In this prospective, observational study, 662 pregnant women scheduled for cesarean delivery provided demographic and clinical information and completed validated psychological and pain assessments. Multivariable hierarchical linear regressions assessed independent associations of demographic, clinical, and psychological characteristics with pain severity and pain interference during the last week of pregnancy.

Results
Women in the study had a mean age of 34 years, and 73% identified as White, 11% as African American, 10% as Hispanic/Latina, and 6% as Asian. Most women (66%) were scheduled for repeat cesarean delivery. Significant independent predictors of worse pain outcomes included identifying as African American or Hispanic/Latina and having greater depression, sleep disturbance, and pain catastrophizing. Exploratory analyses showed that women scheduled for primary (versus repeat) cesarean delivery reported higher levels of anxiety and pain catastrophizing.

Conclusions
Independent of demographic or clinical factors, psychological factors, including depression, sleep disturbance, and pain catastrophizing, conferred a greater risk of late pregnancy pain. These findings suggest that women at higher risk of pain during late pregnancy could benefit from earlier nonpharmacological interventions that concurrently focus on psychological and pain symptoms.
Fabrication of initial trabecular bone-inspired three-dimensional structure with cell membrane nano fragments
Authors: Kadoya Koichi , Hara Emilio Satoshi , Okada Masahiro , Jiao Yu Yang , Nakano Takayoshi , Sasaki Akira , Matsumoto Takuya 
Published: November 2022

Abstract
The extracellular matrix of trabecular bone has a large surface exposed to the bone marrow and plays important roles such as hematopoietic stem cell niche formation and maintenance. In vitro reproduction of trabecular bone microenvironment would be valuable not only for developing a functional scaffold for bone marrow tissue engineering but also for understanding its biological functions. Herein, we analyzed and reproduced the initial stages of trabecular bone formation in mouse femur epiphysis. We identified that the trabecular bone formation progressed through the following steps: (i) partial rupture of hypertrophic chondrocytes; (ii) calcospherite formation on cell membrane nano fragments (CNFs) derived from the ruptured cells; and (iii) calcospherite growth and fusion to form the initial three-dimensional (3D) structure of trabecular bones. For reproducing the initial trabecular bone formation in vitro, we collected CNFs from cultured cells and used as nucleation sites for biomimetic calcospherite formation. Strikingly, almost the same 3D structure of the initial trabecular bone could be obtained in vitro by using additional CNFs as a binder to fuse biomimetic calcospherites.
Impact of pregnancy on progression of preclinical autoimmune disorders: a prospective cohort study
Authors: Beneventi Fausta , Bellingeri Camilla , De Maggio Irene , Cavagnoli Chiara , Boschetti Anna , Giannico Serena , Pandolfi Maria Paola , Spada Carolina , Montecucco Carlomaurizio , Spinillo Arsenio 
Published: November 2022

Abstract

Objectives
The objective of this study was to evaluate the role of pregnancies in the progression from the preclinical phase of autoimmune disorder to a definite rheumatic disease.

Methods
A cohort study of women with symptoms and laboratory findings suggestive for autoimmune disorder were enrolled during the first trimester of pregnancy and followed-up for 5 years with clinical and laboratory assessment. Multinomial logistic regression was used to compute the risk of progression to definite autoimmune disease correcting for confounders.

Results
At the end of follow-up, out of 208 subjects, 81 (38.9%) were considered negative, 53 (25.5%) had symptoms and abnormalities of autoantibody profile compatible with a non-criteria rheumatic status and 74 (35.6%) had a definite rheumatic disease (43 undifferentiated connective tissue disease, 5 systemic lupus erythematosus, 3 SS, 10 antiphospholipid syndrome, and 12 miscellaneous autoimmune disorders). The median time from enrolment to definite diagnosis was 28 months (interquartile range = 18–42). The rate of progression towards a definite autoimmune disease was 47.1% (48/102) among subjects with one or more subsequent viable pregnancies compared with 24.5% (26/106) of those with no subsequent pregnancies (adjusted odds ratio = 4.9, 95% CI: 2.4, 10). The occurrence of preeclampsia during the index pregnancy or subsequent pregnancy was an additional and independent risk factor for progression to a definite autoimmune disease (adjusted odds ratio = 4.3, 95% CI: 1.2, 14.8).

Conclusions
Among women with suspected autoimmune disease during pregnancy, additional viable pregnancies and diagnosis of preeclampsia were independently associated with an increased rate of progression to definite rheumatic disorder. Hormonal modifications associated with pregnancy could worsen preclinical rheumatic disorders favouring their progression to a defined autoimmune disease.
Marketing Malaria Control: Nets, Neoliberalism, and a New Approach to Fighting Malaria
Authors: Moore-Sheeley Kirsten 
Published: November 2022

Summary
This article examines how malaria control in Africa became a neoliberal enterprise that privileged market mechanisms as an ideal way to disseminate health interventions. It does so by tracking efforts to sell insecticide-treated nets on the continent as these tools transformed from cheap stopgap measures intended for primary health care programmes in the early 1980s to a major target of global health aid by the 2000s. Even though experience showed that selling insecticide-treated nets to African populations did not lead to high levels of uptake, malaria programme leaders continued to tinker with market mechanisms to scale up the intervention in line with the interests of prominent donors. Narrating this history as it unfolded across different African countries, this paper shows that the neoliberalisation of malaria control was a contingent and fragmented process that depended as much on local experiences with particular health technologies as it did on high-level policy-making.