bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing a Knowledge-Based System for Diagnosis and Treatment Recommendation of Selected Acute Abdominal Diseases by Collaborating Data Mining Results with Expert Knowledge: The Case of Debre Berhan Referral Hospital
Authors: Adugnaw Demeke , Biredagn Kindie 
Published: November 2022

Abstract
Introduction Acute abdomen is an abnormal condition characterized by sudden onset of severe pain within
the abdomen, which requires immediate diagnosis and treatment. From different acute abdominal
diseases, intestinal obstruction accounts for a great proportion of morbidity and mortality in Africa including Ethiopia. This is due to late presentations, improper health care facilities, and the cause of abdominal pain is not identified timely.
Objective This study aimed to develop a knowledge-based system for the diagnosis and treatment recommendation of selected acute abdominal diseases by collaborating data mining results with the knowledge obtained from the domain experts.
Methodology Design science research methodology (DSRM) used to conduct this research. To extract the required knowledge, the researchers have collected 2260 instances of intestinal obstruction dataset from Debre Berhan referral hospital, and used semi-structured interview technique to acquire from the domain experts. To develop the classifier model, the researchers have conducted four experiments with four different scenarios by using Naïve Bayes, J48, JRip, and PART algorithms.
Result The best performance result achieved by applying PART algorithm on selected attributes under the 10-fold cross-validation test option with 98.19% accuracy. To acquire knowledge from the experts the researchers have used semi-structured interview and used purposive sampling to select domain experts. Finally, After the required knowledge was discovered, the knowledge-based system was developed and the developed system was evaluated Lastly, the evaluation result shows that 91.3% accuracy for the system performance by using test cases and 90.6% for the user acceptance testing.
Conclusion applying the developed knowledge-based system will reduce the morbidity and mortality rate caused by intestinal obstruction. And it will give a significant advantage to the patients, doctors and for the healthcare organizations.
Assessment of Potential Pathogenic Bacterial Load and Multidrug Resistance in Locally Manufactured Cosmetics Commonly Used in Dhaka Metropolis
Authors: Nusrat Namira , Zahra Maftuha Ahmad , Ahmed Akash , Haque Fahim 
Published: November 2022

Abstract
In Bangladesh cosmetics are being produced disregarding the safety rules. So, this study aimed to test the level and nature of bacterial contamination of such cosmetics. A total of 27 cosmetics comprising eight lipsticks, nine powders, and ten creams were bought from New Market and Tejgaon areas of Dhaka city and tested. Bacteria was detected in 85.2% of samples. Majority of the samples (77.8%) exceeded the limit given by the FDA (Food and Drug Administration) and EU (European Union). Both Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella spp.) and Gram-positive bacteria (species of Streptococcus, Staphylococcus, Bacillus and Listeria monocytogenes) were identified. Hemolysis was observed in 66.7% Gram-positive and 25% Gram-negative bacteria. Multidrug resistance was tested in 165 randomly selected isolates. Every species of Gram-positive and Gram-negative bacteria exhibited varying levels of multidrug resistance. The highest levels of antibiotic resistance were in broad-spectrum antibiotics (ampicillin, azithromycin, cefepime, ciprofloxacin and meropenem) and narrow-spectrum Gram-negative antibiotics (aztreonam and colistin). Multidrug resistance was 12%-78% in Gram-negative bacteria and 12%-100% in Gram-positive bacteria. Coagulase and DNase were identified in 97.5% and 5.1% of Staphylococcus aureus isolates respectively. Our findings indicate that these cosmetics pose a risk to the public's health.
A case of APMPPE-like panuveitis presenting with extensive outer retinal layer impairment following COVID-19 vaccination
Authors: Ogino Yo , Namba Kenichi , Iwata Daiju , Suzuki Kayo , Mizuuchi Kazuomi , Hiraoka Miki , Kitaichi Nobuyoshi , Ishida Susumu 
Published: November 2022

Abstract
Background

Vaccination against the worldwide pandemic coronavirus disease 2019 (COVID-19) is underway; however, some cases of newly onset uveitis after vaccination have been reported. We report a case of bilateral acute posterior multifocal placoid pigment epitheliopathy-like (AMPPE-like) panuveitis after COVID-19 vaccination in which the patient’s pathological condition was evaluated using multimodal imaging.

Case presentation

A 31-year-old woman experienced bilateral hyperemia and blurred vision starting 6 days after her second inoculation of the COVID-19 vaccination. At her first visit, her visual acuity was decreased bilaterally, and severe bilateral anterior chamber inflammation and bilateral scattering of cream-white placoid lesions on the fundus were detected. Optical coherence tomography (OCT) showed serous retinal detachment (SRD) and choroidal thickening in both eyes (OU). Fluorescein angiography (FA) revealed hypofluorescence in the early phase and hyperfluorescence in the late phase corresponding to the placoid legions. Indocyanine green angiography (ICGA) showed sharply marginated hypofluorescent dots of various sizes throughout the mid-venous and late phases OU. The patient was diagnosed with APMPPE and was observed without any medications. Three days later, her SRD disappeared spontaneously. However, her anterior chamber inflammation continued, and oral prednisolone (PSL) was given to her. Seven days after the patient’s first visit, the hyperfluorescent lesions on FA and hypofluorescent dots on ICGA partially improved; however, the patient’s best corrected visual acuity (BCVA) dropped to 0.7 OD and 0.6 OS, and the impairment of the outer retinal layer was broadly detected as hyperautofluorescent lesions on fundus autofluorescence (FAF) examination and as irregularity in or disappearance of the ellipsoid and interdigitation zones on OCT, which were quite atypical for the findings of APMPPE. Steroid pulse therapy was performed. Five days later, the hyperfluorescence on FAF had disappeared, and the outer retinal layer improved on OCT. Moreover, the patient’s BCVA recovered to 1.0 OU. Twelve months after the end of treatment, the patient did not show any recurrences.

Conclusions

We observed a case of APMPPE-like panuveitis after COVID-19 vaccination featuring some atypical findings for APMPPE. COVID-19 vaccination may induce not only known uveitis but also atypical uveitis, and appropriate treatment is required for each case.
Knowledgwe and Attitude Regarding Substance Abuce Among Fourth Year Nursing Student at a University, Khomas Region, Namibia.
Authors: Kadhila Joseph Galukeni , Petrus Fransine 
Published: November 2022

Abstract
Introduction: Substance abuse is a pervasive problem across the world. The present study aimed to assess knowledge and attitudes regarding substance abuse among nursing student at the University of Namibia main campus. Substance abuse is a major public health problem globally and its prevalence is increasing rapidly amongst university students in many African countries. The target population were fourth years nursing student between the age of 20 and 35. A quantitative method approach was employed in this study.
Background: Drug and substance abuse is one of the most vexing and pervasive problems that most universities are grappling with in as far as administration is concerned. is a systematic review of literature describing the knowledge of substance abuse by university students and determines their attitude towards substance abuse. It compares university students in different countries.
Aims: The present study aimed to assess knowledge and determined attitudes regarding substance abuse among nursing student at the University of Namibia main campus.
Method: A quantitative descriptive cross-sectional study was carried among 45, 4th year nursing degree students using simple random sampling. A questionnaire was used to collect the data. the researcher personally collected the data. Data was analysed using google docs. Ethical principles were observed during the study.
Results: Finding show that students have adequate knowledge and appropriate attitudes towards substance abuse.
Conclusion: The students had good knowledge of substance abuse yet engaged in substance abuse. The study students frequently used substances like alcohol, cocaine, codeine, cigarette and tramadol. Effective counselling programs is recommended specifically in Universities and families to help tackle this problem.
High-density linkage map constructed from a skim sequenced diploid potato population reveals transmission distortion and QTLs for tuber and pollen production
Authors: Clot Corentin R. , Wang Xulan , Koopman Joey , Navarro Alejandro Thérèse , Bucher Johan , Visser Richard G.F. , Finkers Richard , Eck Herman J. van 
Published: November 2022

Abstract
The reinvention of potato, from a tetraploid clonal crop into a diploid seed-based hybrid crop, requires insight in the mutational load, recombination landscape and the genetic basis of fertility. Genomics based breeding and QTL discovery relies on efficient genotyping strategies such as skim-sequencing, to gather genotypic information. The application of skim-sequencing to full-sib population of non-inbred parents remains challenging. Here, we report on a R implementation of the OutcrossSeq pipeline for diploids and applied it to a large diploid skim-sequenced potato population. We used the resulting bin-markers for the construction of high-density parent specific linkage maps, highlighting variation in parental recombination rate and structural variations. We subsequently explored transmission ratio distortion (TRD) including epistatic ones, indicative of large effect deleterious mutations. Finally, we identified QTLs for seedling tuber yield in pots and pollen production. This study showcases the range of genetic analyses, from marker inference, TRD identification and linkage map construction to QTL mapping, resulting in new insights that contribute to breeding diploid potato.
Using Citizen Science Data to Investigate Annual Survival Rates of Resident Birds in Relation to Noise and Light Pollution
Authors: Pharr Lauren D. , Cooper Caren B. , Evans Brian , Moorman Christopher E. , Voss Margaret , Vukomanovic Jelena , Marra Peter 
Published: November 2022

Abstract
Exponential increases in anthropogenic noise and night lighting have accompanied growth of the built environment. Noise and night lighting cause negative consequences for birds, such as disrupted navigation during migration, mortality from collisions with windows and other infrastructure, and reduced reproductive success, as well as some positive consequences, such as expanded night niches for behaviors associated with feeding, territoriality, and mating. Relatively less is known about noise and light effects on avian annual survival of non-migratory birds, so we carried out an exploratory study to examine variation in adult survival rates of seven avian species in relation to noise and light pollution. We used 20 years of band-resight data collected as a part of the Neighborhood Nestwatch Program (NN), a citizen science project run by the Smithsonian Migratory Bird Center, at 242 sites in greater Washington, D.C. USA. We estimated apparent survival and documented species-specific relationships with light and noise. Gray Catbird (Dumetella carolinensis) and House Wren (Thryothorus aedon) survival decreased and American Robin (Turdus migratorius) survival increased with greater amounts of anthropogenic light. Anthropogenic noise had no relationship with apparent survival for any of the seven species. Life-history trade-offs between survival and reproduction may account for differences in species-specific effects of light pollution. Future research should examine the availability of other fine scale environmental conditions, such as tree canopy cover, that might buffer avian exposure to noise and light pollution.
WITHDRAWN: IRE-1α is a key switch of pyroptosis and necroptosis in mice by dominating Gasdermin D
Authors: Zeng Xin , Zheng Qing , Li Deyong , Li Yumeng , Liu Xiaohong , Zhu Min , Liu Wen , Li Lan , Lu Yanrong , Cheng Jingqiu , Chen Younan 
Published: November 2022

Abstract

Necroptosis and pyroptosis are lytic and inflammatory types of programmed cell death that require the membrane destruction predominantly driven by the mixed lineage kinase domain-like (MLKL) and gasdermin D (GSDMD), respectively. However, the crosstalk between them remains largely unknown. Here, we disclose that inositol-requiring enzyme-1α (IRE-1α) is a potential modulator of both necroptosis and pyroptosis, particularly in liver pathology. In vivo, we found that pharmacological suppression of IRE-1α resulted in serious acute liver failure, which may be attributable to the downregulation of GSDMD and caspase-8 while remarkable upregulation of necroptosis markers receptor-interacting serine/threonine-protein kinase 1 (RIPK1), RIPK3 and MLKL. However, by using thapsigargin (THP) to moderately restore the IRE-1α level, liver failure was distinctly alleviated. Conversely, ER stressor tunicamycin (TM) promoted IRE-1α activity, which initiated liver pyroptosis by increasing GSDMD and NLRP3. But maintaining the IRE-1α balance by moderate inhibition of IRE-1α effectively improved mouse survival. In vitro, we demonstrated that inhibition of IRE-1α led to distinct necroptosis accompanied by the reduction of GSDMD in LO-2 cells and mouse primary hepatocytes. Nevertheless, by using lipopolysaccharide (LPS) to specifically inspire the GSDMD level, necroptosis was obvious ameliorated. In addition, overexpression of IRE-1α in LO-2 cells obviously increased pyroptotic markers, such as GSDMD and NLRP3, but downregulated the necroptosis markers p-MLKL and p-RIPK3. In conclusion, enhanced expression of IRE-1α triggers hepatic pyroptosis, while IRE-1α deficiency activates hepatic necroptosis, and both processes are closed related to the activity of GSDMD in mice. So, IRE-1α may be a promising therapeutic target in tissue injuries.
Novel guidewire-assisted over-the-wire balloon-type versus through-the-scope basket-type endoscopic irreversible electroporation catheter for ablation in the esophagus: an experimental study
Authors: Jeon Han Jo , Choi Hyuk Soon , Lee Jae Min , Kim Eun Sun , Keum Bora , Jeen Yoon Tae , Lee Hong Sik , Chun Hoon Jai , Jeong Seung , Kim Hong Bae , Kim Jong Hyuk 
Published: November 2022

Abstract
Non-thermal irreversible electroporation (NTIRE) is emerging as a promising tissue ablation technique. However, maintaining irreversible electroporation (IRE) electrodes against displacement from the forces of strong esophageal spasms remains an obstacle. Therefore, the present study evaluates the efficacy and safety of newly designed balloon-type endoscopic IRE catheters. Six pigs were randomly allocated to each catheter group, and four ablations were performed on each at alternating voltages (1500 and 2000 V). Esophagogastroscopy was performed during IRE. The ability of balloon-type catheters to deliver full NTIRE sequences was assessed, followed by a histopathologic analysis. The success rate was higher for the balloon- than basket-type catheter (12/12 [100%] vs. 2/12 [16.7%], p < 0.001). Gross inspection and histologic analysis of the 2000-V vs. 1000-V balloon-type catheter revealed a larger mucosal damage area (105.3 mm2 vs. 140.8 mm2, p = 0.004) and deeper damage depth (476 μm vs. 900 μm, p = 0.02). Histopathology of the ablated tissue revealed a separated epithelium, inflamed lamina propria, congested muscularis mucosa, necrotized submucosa, and disorganized muscularis propria. Balloon-type catheters demonstrated remarkable efficacy, achieving full electrical pulse sequences under NTIRE conditions and a safe histological profile below 2000 V (1274 V/cm).
Partial purification of glutathione S-transferase enzyme from the seed of mallow (Malva slyvestris L.) and investigation of the inhibition kinetics of some heavy metals
Authors: Işık Kübra , Taş Ömer , Ekinci Deniz 
Published: November 2022

Abstract
Glutathione S-Transferase (GST) enzyme is abundant in mammals, insects, fish and microorganisms, as well as in various tissues of these species, particularly in tissues exposed to xenobiotics from the environment. As a result, the enzyme execute detoxifying function by scavenging a diverse range of xenobiotics, such as chemotherapeutic medicines, environmental carcinogens, and endogenous compounds. In this study, GST enzyme was purified from mallow (Malva slyvestris L.) seed for the first time and the kinetic parameters were determined. The optimum ionic intensity was found in 400 mM Tris-Buffer, optimum pH: 7.0, and optimum substrate concentration was determined as 0.2 mM. One of the biggest reasons for deterioration of ecological balance in nature is heavy metal accumulation in soil, air and water which becomes a major threat to the vital activities of living things. In this study, the inhibitory effects of Fe+ 3, Cd+ 2, Ag+ and Zn+ 2 heavy metals, which are common in nature, on the Glutathione S-Transferase enzyme purified from mallow seeds were investigated. Each heavy metal showed low micromolar inhibitory effects on enzyme activity. I50 values of the metals were calculated as 0.369, 60.93, 74.602 and 178.22 µM, respectively.
Adaptive Climbing for Functional Motor Assessment of Children with Cerebral Palsy - a Preliminary Study
Authors: simoni greta , monoli cecilia , galli Manuela , colombo Alessandro 
Published: November 2022

Abstract
Adapted sports are known to be a valid complement to classical rehabilitation, especially in the treatment of Cerebral Palsy,which requires continuous rehabilitation to preserve motor function. This paper discusses the preliminary results of the ACCEPT project, which aims at designing an adapted climbing wall which doubles as a tool for the functional assessment of motorfunction. Eight children with hemiplegia were asked to perform climbing games on three consecutive days. A network of forcesensors recorded the force exerted by the children on the holds while climbing, and motion capture was used to determinewhich limb touched which hold. Data was processed into two candidate indices, to be used as proxies for the motor function of the upper and lower limbs. ANOVA suggests that one of the two indices is related to the difference in the functionality of the more and less affected arm, both when collected during structured (p = 0.002) and during unstructured climbing games (p =0.023), while the indices proved inconclusive for the legs. These results suggest that the ACCEPT platform could be used for the automatic functional assessment of the motor function of the upper limbs during a climbing game.
Study on the mechanism of CXCL12/CXCR4 axis mediated upregulation of IL-8 and IL-6 on the biological function of acute T lymphocyte leukaemia cells
Authors:   Liping Zhou1,   Hui Zhao1,   Jing Zhao2,   Xiaowei Zhang1,   Dong Li3,   Guanglei Qian1
Published: November 2022

Abstract
Blocking the CXCL12/CXCR4 axis can alter the biological functions of leukaemia cells. We hypothesised that two cytokines, IL-8 and IL-6, play an important role in this process. We established a co-culture model of leukaemia cells and BMSCs. When AMD3100 and G-CSF blocked the CXCL12/CXCR4 axis, biological changes in the leukaemia cells and changes in IL-8 and IL-6 concentrations were observed. We assume this regulatory process may require the participation of certain signalling pathways. After stimulating the CXCL12/CXCR4 axis, we used specific pathway blockers to determine whether four candidate signalling pathways play regulatory roles in this process. ELISA results confirmed that MG132(10 µm) inhibits the expression of IL-8, confirming that the NF/κB signalling pathway is involved. Perifosine could inhibit the expression of IL-6, confirming that the PI3K/Akt signal system is involved in this process. Western blotting confirmed that changes in the NF/κB signalling pathway resulted in the inhibition of IL-8 expression. SP600125 and Perifosine treatments also played a role. It is possible that the JNK and PI3K/AKT signalling pathways would also inhibit the expression of IL-8, but their inhibition appeared later. IL-6 expression was lower in the Perifosine group. This shows that inhibiting the PI3K/AKT signalling pathway can reduce IL-6 expression. Hence, this process requires the participation of multiple signalling pathways to jointly regulate the expression of IL-8 and IL-6. This type of regulation may be a relatively sophisticated network in which there may be cross-effects. The pathogenesis of leukaemia may be affected by this. IL-6 and IL-8 are physiologically regulated by the CXCL12/CXCR4 axis, the NF/κB and JNK/AP-1 pathways are required for IL-8 expression in T-cell acute lymphoblastic leukaemia. By upregulating IL-8, the bone marrow microenvironment and CXCL12/CXCR4 axis may contribute to the pathogenesis of T-cell acute lymphoblastic leukaemia.
Holographic all-optical waveguide based on photoisomerization
Authors: Liang Jianchu , Long Dafeng , Liu Tong , Wan Kai , Gong Weiping , Qiang Na , Huang Kaijian , Wei Xiaohui 
Published: November 2022

Abstract
The all-optical waveguide theory based on photoisomerization nonlinear effects is systematically and deeply studied, and a nonlinear holographic all-optical waveguide scheme is proposed for the first time. It is found that the induction of a light with stronger isomerization activity on the material weakens the self-defocusing effects of the signal light. Especially, polarization states of both inducing light and signal light also unexpectedly affect propagation of signal light. Part of the theoretical results has been qualitatively confirmed by Z-scan experiments. The proposed holographic all-optical waveguide scheme means polarization information is applied in the all-optical waveguide besides intensity information. Compared with the traditional all-optical waveguide, the performance of holographic waveguide will be greatly improved, making the control of light more precise and easier. Such nonlinear waveguide scheme may find its application in the future all-optical net.