bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation, Identification, and Whole-Genome Sequence Analysis of Pantoea Agglomerans Causing Leaf Spot-Hole Disease in Wild Apricot in Xinjiang, China
Authors: Xu Fangyuan , Liu Liqiang , Liu Jun , He Wei , Liao Kang 
Published: January 2022

Abstract
Wild apricot in Yili wild fruit forest in Xinjiang have been seriously affected by leaf spot-hole disease, with the incidence reaching 100%. To identify the pathogen of apricot perforation in the Yili wild fruit forest, two bacterial strains with strong virulence were obtained by the dilution separation method. The bacterial strains were gram-negative bacteria with yellow colonies, smooth surfaces and neat edges. The results of the pathogenicity test showed that the bacteria could cause symptoms of leaf spot-hole disease in wild apricot, similar to the symptoms in the field, and could cause HR in tobacco. Based on the 16S rDNA gene sequence and multilocus sequence analysis of fusA, gyrB, leuS, pyrG, rpoB and rlpB, combined with the physiological and biochemical characteristics, the isolated strain was identified as Pantoea agglomerans. The pathogen causing bacterial leaf spot-hole disease in wild apricot was determined to be P. agglomerans in the wild fruit forest of Yili, Xinjiang. The whole genome of the pathogen strain GL9-2 was sequenced based on the Illumina HiSeq500 and PacBio RS platforms. The genome size was 4765392 bp, and the G+C value was 55.27%. There was one chromosome and two plasmids in the genome, and 4353 CDs were identified. The annotation results showed that 52 glycoside hydrolase-related genes, 38 bacterial secretory system-related genes and 600 toxin-related genes were predicted.
Comparison of the efficacies of 1.0mm and 1.5mm silicone tubes for the treatment of nasolacrimal duct obstruction
Authors: Nakamura Jutaro , Kamao Tomoyuki , Mitani Arisa , Mizuki Nobuhisa , Shiraishi Atsushi 
Published: January 2022

Abstract

This study was designed to compare the postoperative outcomes of bicanalicular intubation using different diameters of silicone tubes to treat post-saccal nasolacrimal duct obstruction. A total of 130 patients diagnosed with post-saccal obstruction who underwent endoscopic-assisted silicone tube intubation were included in the study. The patients were divided into two groups; those intubated with a 1.5-mm large diameter tube (Group LD) and those with a 1.0-mm normal diameter tube (Group ND). The patency rates of the two groups at one year after tube removal were compared using the Kaplan–Meier's curve and Restricted mean survival time (RMST) method with τ = 365 days. Results showed that the recurrence rate after tube removal was significantly lower in the LD group compared to the ND group (p = 0.001). The patency rates at one year after removal in the LD and ND group were 0.857 (0.754-0.919) and 0.739 (0.617-0.828), respectively. When comparing the patency rates by the RMST method at τ = 365 days, the RMST difference, RMST ratio, and RMTL ratio were higher in the LD group at p = 0.045, 0.052, and 0.046, respectively.
In-sensor optoelectronic computing using electrostatically doped silicon
Authors: Jang Houk , Hinton Henry , Jung Woo-Bin , Lee Min-Hyun , Kim Changhyun , Park Min , Lee Seoung-Ki , Park Seongjun , Ham Donhee 
Published: January 2022

Abstract
Complementary metal-oxide-semiconductor (CMOS) image sensors are a visual outpost of many machines that interact with the world. While they presently separate image capture in front-end silicon photodiode arrays from image processing in digital back-ends, efforts to process images within the photodiode array itself are rapidly emerging, in hopes of minimizing the data transfer between sensing and computing, and the associated overhead in energy and bandwidth. Electrical modulation, or programming, of photocurrents is requisite for such in-sensor computing, which was indeed demonstrated with electrostatically doped, but non-silicon, photodiodes. CMOS image sensors are currently incapable of in-sensor computing, as their chemically doped photodiodes cannot produce electrically tunable photocurrents. Here we report in-sensor computing with an array of electrostatically doped silicon p-i-n photodiodes, which is amenable to seamless integration with the rest of the CMOS image sensor electronics. This silicon-based approach could more rapidly bring in-sensor computing to the real world due to its compatibility with the mainstream CMOS electronics industry. Our wafer-scale production of thousands of silicon photodiodes using standard fabrication emphasizes this compatibility. We then demonstrate in-sensor processing of optical images using a variety of convolutional filters electrically programmed into a 3 × 3 network of these photodiodes.
Whole Genome Sequencing Assembly and Annotation of Halobacillus Trueperi S61 Isolated From the Qarhan Salt Lake
Authors: Li Wei , Shen Shuo , Wang Jian 
Published: January 2022

Abstract
Background: Halophilic microbial as prospective resources of biotechnology due to the advantages of flexible survivability. Qarhan Salt Lake is the second largest Salt Lake in the world which contains rich-unique extremophiles and deserved in-depth exploration. Results: Present study first time isolated novel strain Halobacillus trueperi S61 from Qarhan Salt Lake and performed whole-genome sequencing through combined third-generation PacBio and second-generation Illumina technology. The whole genome of Halobacillus trueperi S61 identified 57549 total reads and consists a complete circular chromosome of 4047887 bp with 43.86% GC content without gaps. Total number of 139 non-coding RNA (included 86 tRNA, 30 rRNA and 23 sRNA), 16 gene islands with 260275 bp and two prophages (with 82682 length) were predicted. In addition, the whole genome of Halobacillus trueperi S61 summarized basic annotation for 3982 protein-coding genes, 3980, 3667, 2998 and 2303 unigenes were annotated with Nr, Swissport, KOG and KEGG database. Combined with advanced analysis, 561 carbohydrate enzymes and 4416 pathogen host interactions related genes were identified. The protein function of Halobacillus trueperi S61 was mainly focus on biological processes, and the protein function was mainly distributed in gene transcription and amino acids, and carbohydrates metabolism. Conclusions: The complete whole genome sequence assembly and annotation of novel strain Halobacillus trueperi S61 isolated from Qarhan Salt Lake mainly focus on protein biological processes and antibiotic resistance, provides a potential resource for biotechnology.
Prevalence of individual brain and eye defects potentially related to Zika virus in pregnancy in 22 U.S. states and territories, January 2016 - June 2017
Authors: Delaney Augustina , Olson Samantha M. , Roth Nicole M. , Cragan Janet D. , Godfred-Cato Shana , Smoots Ashley N. , Fornoff Jane , Nestoridi Eirini , Eckert Valorie , Forkner Allison , Stolz Amanda , Crawford Katherine , Cho Sook Ja , Elmore Amanda , Langlois Peter , Nance Amy , Denson Lindsay , Forestieri Nina , Leedom Vinita O. , Tran Tri , Valencia-Prado Miguel , Romitti Paul , Barton Jerusha E. , John Kristen St. , Mann Sylvia , Orantes Lucia , DeWilde Leah , Tong Van T. , Gilboa Suzanne M. , Moore Cynthia A. , Honein Margaret A. 
Published: January 2022

Abstract
During the Centers for Disease Control and Prevention’s Zika Virus Response, birth defects surveillance programs adapted to monitor birth defects potentially related to Zika virus (ZIKV) infection during pregnancy. Pregnancy outcomes occurring during January 2016-June 2017 in 22 U.S. states and territories were used to estimate the prevalence of those brain and eye defects potentially related to ZIKV. Jurisdictions were divided into three groups: areas with widespread ZIKV transmission, areas with limited local ZIKV transmission, and areas without local ZIKV transmission. Prevalence estimates for selected brain and eye defects and microcephaly per 10,000 live births were estimated. Prevalence ratios (PRs) and 95% confidence intervals (CIs) were estimated using Poisson regression for areas with widespread and limited ZIKV transmission compared to areas without local ZIKV transmission. Defects with significantly higher prevalence in areas of widespread transmission were pooled, and PRs were calculated by quarter, comparing subsequent quarters to the first quarter (January – March 2016). Nine defects had significantly higher prevalence in areas of widespread transmission. The highest PRs were seen in intracranial calcifications (PR=12.6, 95% CI [7.4, 21.3]), chorioretinal abnormalities (12.5 [7.1, 22.3]), brainstem abnormalities (9.3, [4.7, 18.4]), and cerebral/cortical atrophy (6.7, [4.2, 10.8]). The PR of the nine pooled defects was significantly higher in three quarters in areas with widespread transmission. The largest difference in prevalence was observed for defects consistently reported in infants with congenital ZIKV infection. Birth defects surveillance programs could consider monitoring a subset of birth defects potentially related to ZIKV in pregnancy.
ERCP Induced Perforation; A Revisit After Half a Century
Authors: Manasra Abdel Rahman A. Al , Manasreh Tarek , Hammouri Hanan M. , Mesmar Zaid , Husein Anas , Jadallah Khaled , hani Mohammed bani , Farsakh Niazy Abu , Shahwan Shatha K. , Fataftah Jehad 
Published: January 2022

Abstract
Background
One of the most feared complications of endoscopic retrograde cholangiopancreatography (ERCP) is duodenal perforation. We aim to discuss the incidence and risk factors of ERCP induced perforation in light of pertinent literature and review therapeutic options of different perforation types.
Methods
In this case-control study, we reviewed the charts of all patients with ERCP-induced duodenal perforation between January 2016 and June 2020 and compared them to a control group without perforation. Data regarding patients’ demographics, comorbidities, indication for endoscopy, ERCP findings, presumed risk factors for complicated procedures, clinical presentation of perforation, radiologic findings, time to diagnosis and perforation type according to the Stapfer classification were collected. Treatment approach, additional interventions, length of hospital stay (LOS), morbidity and mortality were also documented.
Results
A total of 996 ERCP procedures were performed, 13 patients were diagnosed with ERCP-induced perforation (EIP). The most common indication for ERCP was obstructive jaundice. The most significant risk factor of duodenal perforation was failure or difficult cannulation (P = .003). 5 patients were treated surgically, the majority of them had type I perforation, whereas type IV was the most common in those treated conservatively. ICU admission rates and LOS in the ICU were higher among the surgical patients. The overall mortality rate was 15% with a slightly higher rate in the surgical group.
Conclusion
ERCP-induced duodenal perforation carries significant morbidity and mortality. Conservative management yields favorable outcomes in selected patients compared to the surgical approach.
The circular RNA circGlis3 protects against islet β-cell dysfunction and apoptosis during obesity
Authors: Liu Yue , Yang Yue , Xu Chenying , Liu Jianxing , Chen Jiale , Huang Bin , Yan Wenjing , Pan Yi , Zhang Yanfeng , Pandol Stephen J , Wei Qiong , Zhang Fangfang , Li Ling , Jin Liang 
Published: January 2022

Abstract
The molecular link between obesity and β-cell decompensation that causes diabetes remains incompletely understood. Here we found that circGlis3, a circular RNA derived from Glis3, plays a critical role in islet β-cell compensation. circGlis3 was increased in islets of obese mouse models and moderately diabetic individuals with compensated β-cell function by Quaking (QKI)-mediated splicing. Overexpression of circGlis3 functions to restrain islet β-cell dysfunction and maintain β-cell mass in high-fat diet (HFD) fed mice and Leprdb/db mice. The cellular levels of circGlis3 modulate both insulin synthesis and secretion and lipotoxicity-induced apoptosis, resulting in profound changes in β-cell compensation. In an obesity model, circGlis3 promotes the synthesis and secretion of insulin by upregulating NeuroD1 and Creb1 through sponging miR-124-3p. In addition, we identified SCOTIN and fused in sarcoma (FUS) as interacting proteins using quantitative mass spectrometry. We demonstrated that the binding of SCOTIN to circGlis3 regulated the apoptosis of β-cell. And more, FUS binding to circGlis3 could decrease free circGlis3 in cytoplasm and block mechanism of circGlis3 via abnormal stable formation of stress granules (SGs) in hyperactive response to chronic stresses in obesity that is thought to contribute to the β-cell decompensation. These findings highlight a physiological role for circRNAs in compensation and indicate that modulation of circGlis3 expression may represent a potential strategy to protect against islet β-cell dysfunction and apoptosis during obesity.
Quantitative Investigation of the Morphologically Corrugated CVD-grown Graphene Monolayer Surface with a Nanoparticle-on-Mirror system
Authors: Park Won-Hwa 
Published: January 2022

Abstract
Graphene can be used as a starting material for the synthesis of useful nano-complexes for flexible, transparent electrodes, therapeutic, bio-diagnostics and bio-sensing. In order to apply graphene in the medical field, chemical vapor deposition (CVD) method has been mainly utilized considering its large and near-homogenious carbon constituents. Especially, the less degree of perturbation of graphene monolayer (GM), which is followed by the underneath catalytic Cu surface morphology, is very crucial in terms of providing the suspended GM and relatively fluent lateral carrier mobility with lower sheet resistance value. In this work, we can suggest a surface-Enhanced Raman Spectroscopic (SERS) indicator in a quantitative way on the status of z-directional morphological corrugation of a CVD–grown GM (CVD-GM) by applying a Nanoparticle-on-Mirror (NPoM) system composed of Au nanoparticle (NP) / CVD-GM / Au thin film (TF) plasmonic junction structure. A new (or enhanced) Radial Breathing Like Mode (RBLM) SERS signal around ~150 cm-1 from CVD-GM spaced in NPoM is clearly observed by employing a local z-polarized incident field formed at the Au NP–Au TF plasmonic gap junctions. With this observation, the value of I[out-of-plane, RBLM] / I[in-plane, [2D] at certain domains, it can be suggested as a new optical nano-metrology value to relatively determine between lower z-directional morphological corrugation (or protrusion) status of a CVD-GM spaced in our NPoM system (lower I[RBLM] / I[2D] value) and higher degree of lateral carrier mobility of the CVD-GM associated with lower sheet resistance values as a result of higher blue-shifted Raman in-plane (G, 2D) peak maximum position. Furthermore, we will also expect the bio-sensing performances by utilizing the high specific surface area and ultrahigh flexibility of the CVD-GM in one of the future prospective works such as pressure-strain, strain-to-electricity and chemical-coupled sensor via I[RBLM] / I[2D] values.
Postmortem Study of Organ-Specific Toxicity in Glioblastoma Patients Treated with a Combination of Temozolomide, Irinotecan and Bevacizumab
Authors: Lu Guangrong , Rao Mayank , Zhu Ping , Linendoll Nadine , Buja Maximilian L. , Bhattacharjee Meenakshi B. , Ballester Leomar Y. , Tian Xuejun , Pilichowska Monika , Wu Julian K. , Hergenroeder Georgene W. , Glass Williams F. , Chen Lei , Zhang Rongzhen , Pillai Anil K. , Hunter Robert L. , Zhu Jay-Jiguang 
Published: January 2022

Abstract
Purpose

Systemic monotherapy with temozolomide (TMZ) or bevacizumab (BEV); two-drug combinations, such as Irinotecan (IRI) and BEV, TMZ and BEV and a three-drug combination with TMZ, IRI and BEV (TIB) have been used in treating patients with progressive high-grade gliomas including glioblastoma. Most patients tolerated these regimens well with well-established sides effects of hypertension, proteinuria, and reversible clinical myelosuppression (CM). However, organ-specific toxicities have never been examined by postmortem studies.
Methods

Postmortem tissues (from all major organs) were prospectively collected and examined by standard institution autopsy and brain cutting procedures from 76 decedents, including gliomas (N=68, 44/M, and 24/F) and brain metastases (N=8, 5/M, and 3/F) between 2009 and 2019. Standard hematoxylin and eosin (H&E) were performed on all major organs and brain samples harvested. Electronic microscopic (EM) study was carried on selected subjects kidney samples per standard EM protocol.
Results

Twenty-four glioma subjects were treated with TIB [median: 5.5 (range: 1-25) cycles] at glioma recurrence. Exposure to IRI significantly increased the frequency of CM (p=0.05). No unexpected adverse events were detected clinically or permenant end-organ damage by postmortem examination among subjects who received TIB compared to subjects who received standard of care (SOC) therapies. Among glioma decedents, the most common causes of death (COD) were tumor progression (63.2%, N=43), followed by aspiration pneumonia (48.5%, N=33). No COD was attributed to acute toxicity from TIB. The study also demonstrated that postmortem kidney specimen is unsuitable for studying renal ultrastructural pathological changes due to autolysis.
Conclusion

IRI, but not the extended use of TMZ, significantly increased CM in recurrent glioma patients. There is no permanent organ-specific toxicity among glioma decedents who received prolonged BEV, TMZ or TIB regimen based chemotherapies except expected occasional myelosuppresson. COD are most commonly resulted from glioma tumor progression and aspiration pneumonia.
Morphological characterization and genetic analysis of tri-pistil trait, a precisely regulated pistil number mutation in bread wheat
Authors: Liu Tianxiang , Sun Yulin , Ma Chao , Jiang Wenhui , Wu Hongqi , Jiang Qinqin , Xiao Enshi , Wang Yong , Kang Zhensheng , Shi Xue , Wang Zhonghua 
Published: January 2022

Abstract
Bread wheat (Triticum aestivum L.) is an important source of nutrients for humans. Therefore, improvement of its yields is essential to feed the increasing world population. The tri-pistil (TRP) trait in wheat has a high potential for increasing yields. We obtained a pure tri-pistil wheat line, 4045, and evaluated its morphological properties. The 4045 wheat line stably produced three independently inherited pistils, which led to 1-3 grains in each floret. Among the three pistils, two lately emerged pistils initiated at late anther primordia stage to early tetrads stage. Genetic analysis revealed that there were TRP penetrance variations among the 11 F1 populations of 4045. Fine mapping narrowed the single dominant TRP locus to a 97.3 kb region, containing two candidate genes, on the 2DL chromosome. However, further gene sequence, functional as well as comparative genomic analyses ruled out the only two candidate genes. Therefore, TRP is high-likely a unique gain-of-function mutation that does not exist in normal wheat genome. Transcriptome analysis of floral homeotic genes revealed that expressions of the C-class TaAG-2s, which are essential for carpel specification, significantly increased in 4045, implying that TaAG-2s have played important roles in TRP-regulated tri-pistil formation. This study highlights that TRP leads to a precisely regulated pistil number increase (PRPNI) mutations and proposed a regulatory model of PRPNI pistil architecture. PRPNI offers a novel abnormal pistil development resource for research of floral architectures and potential on crop yield improvement.
Intense Pulsed Light Combined With Meibomian Gland Massage for Recurrent Corneal Erosion: a Review of Clinical Effectiveness
Authors: Xu Man , Niu Xiaoguang 
Published: January 2022

Abstract
Purpose: To observe the therapeutic effect on recurrent corneal erosion syndrome (RCES) by intense pulsed light (IPL) combined with meibomian gland massage. Methods: We recruited 30 patients (30 eyes) with RCES as the RCES group and 31 patients (31 eyes) as the control group. Both the groups received eyelash sampling, Demodex count, meibomian gland infrared photography, and the blepharolipin score. The RCES group was divided into the treatment group and the observation group again. Results: Parameters in the RCES group were higher than those in the control group. The parameters of the treatment group were decreased compared with the observation group. During the follow-up period, one patient in the treatment group relapsed. Conclusion: Meibomian gland dysfunction and Demodex infection may be associated with RCES. IPL combined with meibomian gland massage can significantly improve meibomian gland function, reduce the number of Demodex, and effectively control the relapse of RCES.
Correlation Between Cyclin D1 Expression With Different Pathological Changes in Colorectum Patients
Authors: Mohammed Hanaa Ibrahim Salih , Elsheikh Mohammed Abdelgader , Idris Alkhair 
Published: January 2022

Abstract
Background: Cyclin D1 plays a vital role in cancer cell cycle progression and is overexpressed in many human cancers, including colorectal cancer. Objectives:This study was aimed to detect cyclin D1 in colorectal cancer patients and to correlate cyclin D1 expression with different pathological changes in colorectum.Methods: Tissues microarray paraffin block with 48 colorectal cancer samples were retrieved from the archives of Elrahma Medical Center. The cyclin D1 was analyzed.Results:Cyclin D1 did not correlate with pathological alterations and with tumor grade.Conclusion:Results indicated that; cyclin D1 not correlates with pathological alteration of colorectal cancer.