bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating water quality transformation and the dominant driving mechanism of rivers under sluice scheduling
Authors: Mi Qingbin , Dou Ming , Xia Rui , Li Guiqiu , Liang Li 
Published: June 2022

Abstract
The complicated biogeochemical behavior of matters in the water environment caused by sluice scheduling result in the transformation of water quality to present the characteristics of multi-media, multi-phase and multi-form. Based on the complex reaction mechanism induced by sluice scheduling in water environment system, a mathematical expression describing the mass transfer process of water components between various phases was proposed, a multi-phase transformation model of water quality with certain physical mechanism was established, and the water quality concentration under different sluice scheduling situations were simulated. By comparing with the no sluice scenario, the water quality concentration change rate under different sluice scheduling situations was calculated; the role of sluice scheduling in the transformation process of water quality was quantitatively evaluated; the driving effect of sluice scheduling on various reaction mechanisms in water was analyzed; and the dominant driving mechanism was identified. This study found that the change in pollutant concentration was significantly affected by sluice scheduling. Furthermore, the water quality concentration change rate was inversely associated with the change in the gate opening height, and the water quality concentration change rate in dissolved phase was larger than that in other phases. The strength and weakness of different dominant reaction mechanisms changed alternately with a change in sluice scheduling. For Huaidian Sluice, when the gate was fully open at 18 holes and the opening was approximately 40 cm, the contribution ratio of each corresponding reaction mechanism in water quality transformation was approximately same, and the dominance was relatively balanced, which could effectively suppress an increase in the nitrogen content and reduce the risk of nitrogen pollution.
Efficacy of Medical Legal Partnerships to Address Health Harming Legal Needs: A Systematic Review of Experimental Studies in the Field
Authors: John Jemima , Murphy Thomas , Zientek Emily , Nguyen Thien-An , Liaw Winston , Burnett Catherine , Aitken Mary , Sharma Shreela 
Published: June 2022

Abstract
Objectives
Medical legal partnerships (MLPs) function by leveraging partnerships between legal and medical entities to holistically target the health harming legal needs (HHLNs) of vulnerable patients. Yet, despite their documented success, robust experimental-based research is still lacking on their efficacy. This review synthesizes the findings of MLP interventions and identifies gaps and opportunities for MLP expansion across vulnerable communities.
Methods
A comprehensive search was conducted across Ovid MEDLINE, PubMed, Google Scholar, Westlaw and LexisNexis databases between January 12th 2021 and April 25th, 2021. Articles were eligible if they used an experimental design, tested the effects of an MLP intervention on HHLNs, and had full text for review, with no restrictions on publication year, place, or population (adult or child). Two reviewers independently screened articles for inclusion in Rayyan QCRI- a systematic review tool and also independently assessed the quality (risk of bias) of each study.
Results
Our search yielded 1267 articles; six studies were ultimately eligible. Populations were largely racially-ethnic minorities, women, of lower socioeconomic status and with HHLNs including food and housing insecurity, unemployment, immigration, and education concerns. All studies’ MLPs demonstrated positive effects on primary endpoints including perceived stress, diabetes, immunization rates, and additional child health outcomes. Two experimental studies had low bias, while the four remaining studies (quasi-experimental) had moderate risk of bias.
Conclusion
Studies highlighted the promising ability of MLPs to address HHLNs. However, additional rigorous research in MLPs is needed to establish an evidence-based approach to addressing social needs and health outcomes.
A cross-sectional national investigation of COVID-19 outbreaks in nurseries during rapid spread of the Alpha (B.1.1.7) variant of SARS- CoV-2 in England
Authors: Aiano Felicity , McOwat Kelsey , Obi Chinelo , Powell Annabel A , Flood Jessica , Bhardwaj Shivraj , Stoker Kelly , Haskins Donna , Wong Brian , Bertran Marta , Zavala Maria , Bosowski Johanna , Jones Samuel , Amin-Chowdhury Zahin , Coughlan Laura , Sinnathamby Mary , Zaidi Asad , Merrick Rachel , Zhao Hongxin , Ismail Sharif , Ramsay Mary E , Ladhani Shamez N , Saliba Vanessa 
Published: June 2022

Abstract
Background
In England, the emergence the more transmissible SARS-CoV-2 variant Alpha (B.1.1.7) led to a third national lockdown from December 2020, including restricted attendance at schools. Nurseries, however, remained fully open. COVID-19 outbreaks (≥ 2 laboratory-confirmed cases within 14 days) in nurseries and assess the risk of SARS-CoV-2 infection and incidence rates in staff and children over a three-month period when community SARS-CoV-2 infections rates were high and the Alpha variant was spreading rapidly across England.
Methods
This was a cross-sectional national investigation of COVID-19 outbreaks in nurseries across England Nurseries reporting a COVID-19 outbreak to PHE between November 2020 and January 2021 were requested to complete a questionnaire about their outbreak.
Results
324 nurseries, comprising 1% (324/32,852) of nurseries in England, reported a COVID-19 outbreak. Of the 315 (97%) nurseries contacted, 173 (55%) reported 1,657 SARS-CoV-2 cases, including 510 (31%) children and 1,147 (69%) staff. A child was the index case in 45 outbreaks (26%) and staff in 125 (72%) outbreaks. Overall, children had an incidence rate of 3.50% (95%CI, 3.21–3.81%) and was similar irrespective of whether the index case was a child (3.55%; 95%CI, 3.01–4.19%) or staff (3.44%; 95%CI, 3.10–3.82%). Among staff, incidence rates were lower if the index case was a child (26.28%; 95%CI, 23.54–29.21%%) compared to a staff member (32.98%; 95%CI, 31.19–34.82%), with the highest incidence rate when the index case was also a staff member (37.52%; 95%CI, 35.39–39.70%). Compared to November 2020, outbreak sizes and incidence rates were higher in January 2021, when the Alpha variant predominated. Nationally, SARS-CoV-2 infection rates in < 5 year-olds remained low and followed trends in older age-groups, increasing during December 2020 and declining thereafter.
Conclusions
In this cross-sectional study of COVID-19 outbreaks in nurseries, one in three staff were affected compared to one in thirty children. There was some evidence of increased transmissibility and higher incidence rates associated with the Alpha variant
Deep-Cloud: A Deep Neural Network-Based Approach for RNA-seq Gene Expression Analysis
Authors: Ge Qinyu , Zhou Ying , Jia Erteng , Shi YHuajuan , Liu Zhiyu , Sheng Yuqi , Pan Min , Tu Jing , Lu Zuhong 
Published: June 2022

Abstract
Background
Presently, the field of differential expression analysis of RNA-seq data is still in its infancy with new approaches constantly being proposed. Combining deep neural networks to explore gene expression information in RNA-seq data provides a novel possibility in this field.
Results
This study focuses on the development of a deep neural network-based RNA-seq gene expression analysis model, named Deep-cloud. Its main advantage is not only the excellent construction of deep learning network models using convolutional neural network and long short-term memory to predict the gene expression of RNA-seq, but also the more in-depth analysis of the differential gene expression between disease and normal groups of transcriptome data by combining the statistical methods of cloud model.
Conclusion
Comparing the results of this data analysis with those obtained by traditional differential gene analysis software such as DESeq2 and edgeR, the Deep-cloud model further improves the sensitivity of obtaining differential expressed genes. Overall, the proposed Deep-cloud paves a new pathway for the biomedical field.
Assessment of psychosocial wellbeing of parents &amp; Quality of Life (QoL) of children with 46, XY Differences of Sex Development (46, XY DSD) followed up in endocrine clinics at Lady Ridgeway Hospital for Children (LRH), Colombo, Sri Lanka
Authors: Prematilake Dilusha Chaturani , Koralage Buddhika Mahesh Pasyodun , Atapattu Navoda , de Silva Varuni , de Silva Shamya 
Published: June 2022

Abstract
Objectives46, XY Differences of Sex Development imposes potential psychological trauma to parents and poor quality of life (QoL) of children. Primary objective of this study was to assess, psychosocial wellbeing of parents of children with 46, XY DSD and Quality of Life of children with 46, XY DSD aged more than 12 years. Secondary objective was to describe clinical profile of 46, XY DSD patients followed up in Lady Ridgeway Hospital for Children.Results 28 (53%) mothers and 23 (45%) fathers had psychological-morbidity with GHQ-30. Patients’ age was negatively correlated with GHQ-scores of both parents (p<0.05). Median (IQR) psychosocial wellbeing scores for mother (44.0) and father (50.8) were positively correlated (p<0.001). Psychosocial wellbeing scores were negatively associated with age of child (p<0.01). Median (IQR) scores of PaedQL were 75.0 (60.8-78.3) and 73.3 (58.3-80.8) for parental and child components. Scores of parental components were negatively associated with age of child (p<0.01). Among school-aged children,17 children (65.4%) were not drinking water from school-sources, 15 (57.7%) did not use washrooms and 17(65.4%) did not participate in sports. 46, XY DSD associated with significant psycho-social morbidity in parents and psychological distress in children.
Persistence and Effectiveness of Atrazine Herbicide in Sweet Corn (Zea mays saccharata Sturt) Cultivation with Different Organic Ingredients in Doses
Authors: Sumekar Yayan , Widayat Dedi , Umiyati Uum , Fitri Alifhia 
Published: June 2022

Abstract
The presence of weeds in sweet corn is one of the obstacles that can reduce crop yields, this is due to competition in taking nutrients, light, growing space, and water. Weed control is mostly done by using the herbicide atrazine. Herbicide persistence in the soil is strongly influenced by the organic matter content in the soil. The research objectives were to obtain the highest yield of sweet corn with the application of the herbicide Atrazine, as well as to determine the persistence of the herbicide used at each prescribed application dose. The results showed that atrazine herbicide treatment starting at a dose of 1–2,6 kg a.i./ha had an effect on reducing total weed dry weight and corn cob weight. The application of organic matter can increase the rate of herbicide degradation in line with the increased activity of microorganisms in the soil.
Preclinical mouse studies of vector immunity and long-term responses to CORAVAX, a Rabies virus-vectored COVID-19 vaccine
Authors: Schnell Matthias , Yankowski Catherine , Wirblich Christoph , Kurup Drishya 
Published: June 2022

Abstract
The objective of this study is to further analyze the recombinant Rabies virus-vectored SARS-CoV-2 vaccine, CORAVAX, as an effective COVID-19 vaccine strategy. CORAVAX has proven immunogenic and protective against SARS-CoV-2 in animal models. Here, we have screened adjuvants for the highest quality antibody titers, negated the concern of pre-existing Rabies-vector immunity, and established its potential as a long-term COVID-19 vaccine. We have tested TLR4 agonists, inflammasome activators, and Alum adjuvants in CORAVAX and found TLR4-activating MPLA-AddaVax to have the greatest potential. We followed the humoral immune response to CORAVAX in mice with pre-existing Rabies virus immunity and saw no significant differences compared to naïve mice. We then followed the immune response to CORAVAX over several months and 1 year post-immunization. Mice maintained high antigen-specific serum antibody titers as well as long-lived antibody-secreting cells in the spleen and bone marrow. We believe this Rabies-vector strategy combats the problem of waning immunity of other COVID-19 vaccines. These results together support CORAVAX’s potential during the ongoing COVID-19 pandemic.
Urinary exosomal hsa_circ_0001250 as a novel diagnostic biomarker of idiopathic membranous nephropathy.
Authors: Li Qianyu , Xu Mingzhu , Zhang Zhiping , Yin Min , Zhang Yucheng , liu feng 
Published: June 2022

Abstract
Aims
Idiopathic membranous nephropathy (IMN) is a common cause of adult nephrotic syndrome. Currently, the diagnosis of IMN mainly depends on renal biopsy, which is invasive. What’s more, markers already known for the clinical diagnosis of IMN are not sensitive enough. The present study aims to investigate the profiling of urinary exosomal circular RNAs (circRNAs) of IMN, and to look for a potential biomarker for diagnosis of IMN.
Methods
Urine exosomes were collected from patients with IMN and idiopathic nephrotic syndrome (INS), as well as healthy controls (HCs) by ultracentrifuge. A pairwise comparison between 5 IMN and 5 HC was performed by high-throughput sequencing. Enrichment analysis were performed to explore the potential functions of differentially expressed circRNAs in IMN. Among three differentially expressed circRNAs which may be involved in signaling pathways of pathogenesis of IMN and matched conserved mouse circRNAs, hsa_circ_0001250 was selected as the target circRNA after quantitative polymerase chain reaction among 23 IMN, 19 INS and 23HC. Sanger sequencing and RNase R digestion assay were performed to validated the ring-structure and sequence of hsa_circ_0001250. ROC (Receiver Operating Characteristic) curve correlation analysis was used to further validate the potential utility of hsa_circ_0001250 as a diagnostic biomarker of IMN. A circRNA-miRNA-mRNA network was constructed to reflect the relationship between hsa_circ_0001250 and its target miRNAs and mRNAs.
Results
766 up-regulated and 283 down-regulated circRNAs were identified in IMN patients. Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed signaling pathways of pathogenesis of IMN which the different expressed circRNAs may participate in. The ring-structure and the sequence of hsa_circ_0001250 were confirmed, the expression of hsa_circ_0001250 was validated significantly increased in IMN, relevant with high level of proteinuria. A circRNA-miRNA-mRNA network reflected that hsa_circ_0001250 may play a role in the pathogenesis of IMN by target hsa-miR-639 and hsa-miR-4449.
Conclusion
We revealed the expression and functional profile of differentially expressed urinary exosomal circRNAs of IMN patients. Urinary exosomal hsa_circ_0001250 was tested as a potential biomarker of IMN and a predicted circRNA-miRNA-mRNA network was constructed.
CLEC10A is a prognostic signature and associated with tumor microenvironment in breast cancer and experimental verification
Authors: FANG zhixin , WU Hong-yan , LIU Jia-qing , WU Hui , SHEN Wen-bin , WANG Hui-ying , XING Xue-kun 
Published: June 2022

Abstract
Background: Breast cancer (BRCA) is a high risk malignancy among women worldwide, finding valuable biomarkers for BRCA prevention and treatment has become a top priority.CLEC10A belongs to the C-type lectin domain family, plays the leading action in the initiation of immune response and is of great value in tumor targeted immunotherapy, but the prognostic importance and immunomodulatory mechanism of CLEC10A in BRCA have not been reported.Methods: Data from TCGA and GTEx and immunohistochemistry were recruited to check the differential expression of CLEC1OA1 in BRCA, as well as its clinicopathological features, prognostic value, protein network interaction and functional enrichment analysis, and immune infiltration analysis.Results: We confirmed that the decreasing expression of CLEC10A was linked with dismal prognosis in BRCA, and the expression of CLEC10A was positively related with the infiltration condition of important immune cells.Conclusions: These findings provide an important basis for CLEC10A as a latent immunotherapy target and prognostic judgement for BRCA.
Plasmonic biosensor for the study of blood diseases by analysis of hemoglobin concentration
Authors: Ilyes HABIA Mohamed , aissa manallah , khaled ayadi 
Published: June 2022

Abstract
Plasmonic materials and phenomena are widely studied and applied in multiple fields for an extended time. One among the foremost promising applications is within the engineering of biosensor devices, Diseases of the red blood cells are among the diseases of the blood. Anemia as an example is an abnormal reduction within the concentration of hemoglobin within the blood. When the amount of red blood cells decreases, so does the concentration of hemoglobin. The tissues and organs not receive sufficient oxygen to work normally. This numerical study will give a contribution or approach on the behavior of blood with surface plasmons resonance SPR by analyzing the performance of SPR with any changes in hemoglobin concentrations (refractive index) for the application of technique in the detection of blood diseases.
Threshold of Anthropogenic Sound Levels Within Protected Landscapes in Kerala, India for Avian Habitat Quality and Conservation
Authors: Rajan Sajeev C , M Vishnu , Mitra Ahalya , P Sooraj N , K Athira , M Somasekharan Pillai , R Jaishanker 
Published: June 2022

Abstract
Anthrophony is an important determinant of habitat quality in the Anthropocene. Acoustic adaptation of birds at lower levels of anthrophony is known. However, threshold anthrophony, beyond which biophony starts decreasing, is less explored. Here, we present empirical results of the relationship between anthrophony and biophony in four terrestrial soundscapes. The constancy of the model predicted threshold vector normalised anthropogenic power spectral density 0.40; at all the study sites is intriguing. The results pave the way to determine permissible sound levels in protected landscapes.
IL-10/STAT3 signalling pathway-mediated T-cell suppressors upregulation in severe HFMD with EV71 infection
Authors: Zhang Yaozhong , Peng Qi , Yuan Xu , Lin Zitian , Jiang Zaixue , Zhang Yinghong , Guo Xing , Lu Xiaomei , Zhong Baimao 
Published: June 2022

Abstract
Enterovirus 71 (EV71) causes outbreaks of hand, foot and mouth disease (HFMD) in many countries, especially in the Asia-Pacific region. The EV71 engages diverse strategies to escape immune responses during infections. Arg-1, iNOS and COX-2 are particular suppressors secreted by MDSCs to inactivate T cells, which have been considered to play a significant role in the immune escape of viruses or malignant tumours. However, whether EV71 escapes the immune response through T-cell suppressors remains unknown. We established an animal model of HFMD by EV71 infection in BALB/c mice. The expressions of cytokines IL-10 and Arg-1, iNOS and COX-2 were detected by ELISA and qRT-PCR. The phosphorylation of STAT3 was detected by western blot. The number of MDSCs in PBMCs was examined by flow cytometry. The number of MDSCs in HFMD mice was significantly higher than that in the control, which is in agreement with the upregulation of T-cell suppressors (Arg-1, iNOS and COX-2) secreted by MDSCs in the infection group. The percentage of the gMDSC subpopulation was significantly increased in severe HFMD mice. The serum level of IL-10 and p-STAT3 were upregulated significantly in the infected group whilst inhibition of IL-10 and STAT3 led to the reduction in the T-cell suppressors. Our results suggest that T-cell suppressors (Arg-1, iNOS and COX-2) upregulated in HFMD in response to EV71 infection is attributable to STAT3 signalling activation triggered by IL-10 induction. We speculate that it was caused by elevation of MDSCs to facilitate the immune escape of EV71 in severe HFMD.
Differential gene expression analysis of the whole blood transcriptome between young and old companion border collie dogs
Authors: Jónás Dávid , Tátrai Kitti , Egyed Balázs , Kubinyi Eniko 
Published: June 2022

Abstract
BackgroundAging is the most significant risk factor in many diseases and for mortality alike, and it is known to be influenced by both genetic and environmental factors. Due to dogs’ importance in human societies, the study of aging in companion dogs is worthwhile in its own right. Still, dogs could also be ideal translational model animals for human aging research. In this study, our primary goal was to investigate the gene expression changes with age in whole blood samples of dogs. The advantage of using blood is its accessibility and known biomarkers for diseases.MethodsWe sequenced the poly(A)-tailed RNA fraction in the blood samples of five young and five old companion border collie dogs and implemented a differential gene expression analysis. We removed haemoglobin related reads in silico, following standard raw data quality control and alignment to the reference genome. Sample collection and preparation, sequencing and data analysis were implemented using standard laboratory methods and RNA-seq data analysis pipelines.ResultsThe estimated statistical power of the study was 98% according to an initial power analysis with a 10% false discovery rate and minimum fold change of 3. Raw sequence data quality was high with an 86% alignment rate to the CanFam 3.1 dog reference genome. Following in silico haemoglobin read removal, 23-74 million reads remained for the differential gene expression analysis. In contrast to our expectations, we could not differentiate age clusters in either a multidimensional scaling analysis or in a principal component analysis. We found only a limited number of differentially expressed genes (n=61) between young and old dogs’ blood transcriptome. The only significant gene ontology term was response to bacterium with a fold change of 8.62.ConclusionsIn contrast with the large number of differentially expressed genes in the prefrontal cortical brain region of dogs, we could identify only one blood biomarker for aging in blood. Confounding factors (e.g., diet) might have influenced our results. Future research could also use biological age instead of the animals’ chronological age.
A Novel UWB Flexible Antenna with Dual Notch Bands for Wearable Biomedical Devices
Authors: Geyikoglu M. Dilruba 
Published: June 2022

Abstract
This study presents a novel UWB flexible antenna with dual band-notched design for wearable biomedical devices. The proposed antenna is designed on Kapton Polyimide-based flexible substrate. This includes a CPW fed circular and triangle structure. The dual notched bands are realized by using two triangular-shaped defected ground structures. The first notched band (2.4−3.7 GHz) is generated for rejecting WLAN and WiMAX, the second notch (5.15-5.725 GHz) is generated for rejecting HyperLAN /2. The designed UWB antenna has approximately a bandwith of 150% (2.05-14 GHz) in simulation. Thus, the designed UWB antenna meets FCC standards. The antenna has an omnidirectional radiation pattern with a maximum gain of 12.7 dB in 8.4 GHz.The proposed antenna is fabricated with the low-cost airbrush printed technique. In this technique, a higher gain value is obtained by controlling the thickness of the conductive layer .Effect of flexibility on the antenna performance is tested for different configurations in the simulation and anechoic chamber environments. According to the results obtained, the overall performance is not affected except for the shift in frequency. Since the antenna has a UWB structure, the frequency shift that occurs in bending is at a tolerable level. The proposed UWB antenna is suitable for wearable biomedical devices, with a high UWB performance.