bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation the Effect of Royal Jelly on the Growth of Two Members of Gut Microbiota; Bacteroides fragillis and Bacteroides thetaiotaomicron
Authors: Kazemi Vida , Mojtahedzadeh Mojtaba , Siadat Seyed Davar , Hadjiakhondi Abbas , Ameri Abdulghani , Ahmadi Badi Sara , Manayi Azadeh , Bagheri Mahdi 
Published: March 2022

Objective: In this study the effect of Royal jelly on the growth of two important members of Bacteroides spp.; Bacteroides fragilis and Bacteroides thetaiotaomicron, was evaluated. Also the physicochemical properties and cytotoxicity effects of Royal jelly on Caco-2 cell line as gastrointestinal epithelial cell model, assessed.
Methods : Bacteria, Bacteroides fragilis and Bacteroides thetaiotaomicron were grown on brain heart infusion (BHI) broth medium supplemented with Royal jelly in 3 different concentrations (2.5, 5 and 10% v/v), both of the bacteria (1.5Ã—108 cfu/mL) were inoculated to BHI broth contained Royal jelly in anaerobic condition. To calculate the bacterial optical density (OD), the absorbance was measured at 600 nm after an overnight. Also Caco-2 cells, was used to study the effects of Royal jelly on epithelial cell viability, and the Physicochemical properties consist of total proteins, polysaccharides, phenolic compounds, total lipids, ash and moisture by UV-VIS spectrophotometric and gravimetric methods were evaluated .
Results: The growth of B. fragillis and B. thetaiotaomicron were increased by Royal jelly (2.5, 5 and 10% v/v concentrations) and the results indicated that Royal jelly increased the growth of bacteria in a dose dependent manner (p<0.001). In addition MTT assay showed more than 95% viability of Caco-2 cells treated with Royal jelly. The Iranian Royal jelly sample contains 59.01% water, 11.57% proteins, 12% lipids, 12.63 % polysaccharide and 5% mineral.
Conclusion: The present study showed that Royal jelly has a potential effect in the preserving gut microbiota and it is suggested that Royal jelly as a complementary and alternative medicine can be used to treatment diseases are associated with gut microbiota- host interactions and immune regulating. Although we need to expand our knowledge by designing clinical trials to conï¬rm the therapeutic effects of Royal jelly on gut microbiota modulation as a barrier function.
Fibrin-collagen hydrogel as a scaffold for dermoepidermal skin substitute, preparation and characterization
Authors: Mollapour Sisakht Mahsa , Nilforoushzadeh Mohammad Ali , Verdi Javad , Banafshe Hamid Reza , Safaei Naraghi Zahra , Mortazavi-Tabatabaei Seyed Abdolreza 
Published: March 2022

Objective: Bioengineered skin substitutes were created to address wound healing problems. Skin substitutes contains live human cells seeded onto a matrix to provide cytokine, growth factor and other proteins from ECM required to decrease healing time. These products are classified based on their durability, the cells seeded on them and their originality. In this study, we aimed to investigate fibrin-collagen hydrogel as a new scaffold to design a bilayer temporary skin equivalent.
Methods: Fibrin gel was prepared by crosslinking fibrinogen with thrombin and mixing it with collagen type 1. Human fibroblasts and keratinocytes were isolated from skin biopsies of healthy donors and foreskin and the cells were seeded onto 3D hydrogel layer by layer. Morphological assessment, histological analysis, immunocytochemistry were performed to characterize the scaffold properties.
Result: The results of scaffold characterization demonstrated good porosity, cell viability and biocompatibility of the scaffold.
Conclusion: Fibrin-collagen as a natural material in organotypic cell culture modeling demonstrates that hydrogel scaffolds can be properly designed to generate bilayer or composite temporary skin grafts.
Effect of Artemisia fruit extract on TNF- Î± and IL-6 levels in streptozotocin-induced diabetic mice
Authors: Ali Ekhlass N. , Eltyeaf Emad Mahmoud , Kadhem Ashwaq A. , Jouda Jamela 
Published: March 2022

Objective: The present study was conducted to investigate the effects of Artemisia extract on some immunological parameters in streptozotocin-induced diabetic mice.
Methods: After preparation of Artemisia extract, many chemical tests were used to identify the type of element and compounds presented in this plant using many chemical techniques. Thirty five streptozocin (STZ) induced diabetes mice were divided in to 5 groups; the first group provided only with water, the other four groups were consumed orally ingested the plant extract in four different concentration (2000, 1000, 500,250) mg/Kg of body weight. Another 10 mice didnâ€™t injected with STZ were divided in to two groups; one consumed Artemisia (Art group), and the second consumed only normal saline (Cont. group). After 14 days of diabetes induction and Artemisia extract treatment, the mice were sacrificed. Blood and tissue (brain, spleen and kidney) were collected. Fasting blood sugar and insulin levels were determine in the serum. Furthermore Tumor Necrosis Factor- alpha (TNF- Î±) and Interleukin-6 (IL-6) levels were determined in the serum and aliquots of homogenize tissues.
Results: Results declared that the extract of Artemisia fruit contains high levels of active compounds especially antioxidant compounds. IL-6 and TNF-Î± levels were decreased while insulin and glucose levels were increased in the STZ- induce mice group. Artemisia extract effects differently on glucose and insulin levels depend on its concentration. Interestingly, IL-6 and TNF-Î± levels increase in serum, brain and spleen of the STZ-induce mice group consumed different concentration of Artemisia but it normalized in the STZ-induce mice group consumed 250 mg/kg Artemisia, as well as insulin and glucose levels for the same group, while there was no difference in kidney.
Conclusion: Artemisia can control diabetes in 1000 and 500mg/Kg through controlling insulin level, and in the other hand, using the plant extract in 250mg/Kg ,acts as immune modulator for anti-inflammatory agents IL-6 and TNF-Î±.
Pomegranate protects renal proximal tubules during gentamicin induced-nephrotoxicity in rats
Authors: Alkuraishy Hayder M. , Al-Gareeb Ali I. , Al-Naimi Marwa Salih 
Published: March 2022

Objective: The aim of present study was to evaluate the nephroprotective effect of pomegranate in gentamicin induced-nephrotoxicity in rats.
Methods: Thirty Sprague Dawley Male rat were used, which randomly divided into 3 groups, 10 rats in each group. Group1 (n=10): Rats treated with distilled water for 12 days.Group2 (n=10): Rats treated with distilled water + Gentamicin .Group3 (n=10): Rats treated with pomegranate + gentamicin. Blood urea, serum creatinine, serum malondialdehde (MDA), superoxide dismutase (SOD) , glutathione reductase (GSH) , Neutrophil Gelatinase Associated Lipocalin (NGAL) , kidney injury molecules (KIM-1) and Cystatin-c were measured .
Results: Blood urea and serum creatinine were significantly elevated in gentamicin group compared to the control p=0.007 and p=0.04 respectively. Also estimated GFR was significantly decreased in gentamicin group compared to the control p=0.04. Cystatin-c serum level and MDA serum level were increased but SOD and GSH were decreased insignificantly in gentamicin group compared to the control p>0.05. Pomegranate reduced blood urea significantly p=0.002. Pomegranate improves endogenous anti-oxidant capacity since; it increase GSH significantly compared to gentamicin group p=0.02. There were insignificant effects on MDA and SOD. Moreover, pomegranate reduce serum levels of KIM-1and NGAL in gentamicin group p<0.05.
Conclusion: Pomegranate attenuates gentamicin induced-nephrotoxicity through potentiation of endogenous anti-oxidant capacity and inhibition of inflammatory pathway.
Cord Blood Sex Hormones Concentration: Relation to Birth Weight and Pregnancy Complications
Authors: Al-Omary Hanan L. , Alawad Zainab M. 
Published: March 2022

Objectives: Umbilical cord blood can be taken at birth and largely gives indication of fetal and maternal conditions. The aim of the study was to investigate the relation between sex hormones in cord blood and birth weight of newborns and pregnancy complications.
Materials and methods: Fifty cord blood samples were collected from newborns at labor room of Baghdad Teaching Hospital between May and October 2018. Blood was withdrawn from their mothers for lead analysis. Five milliliters (ml) of cord blood was taken, 3 ml was used for testosterone and estradiol analysis (using enzyme-linked immunosorbent assay) and 2 ml for lead measurement by lead care analyzer. Newborns weight and head circumference were measured. Delivered women were divided into 4 groups: Women with normal pregnancy, women with preeclampsia, diabetic women and polycystic ovary syndrome (PCOS) women.
Results: There was no significant difference in age between women in all groups (pËƒ0.05). Birth weights, estradiol, and testosterone were significantly different between groups. Estradiol was higher in cord blood of newborns of PCOS women (pË‚0.05) than others. Testosterone was higher in cord blood of babies of PCOS and preeclampsia women compared to those of diabetes (pË‚0.05). There were no significant differences between male and female neonates regarding cord estradiol (3596.27Â±1934.69, 3714.57Â±1581.47 pg/ml respectively), and testosterone (393.18Â±87.14, 361.43Â±102.14 ng/ml respectively) (pËƒ0.05). Maternal lead levels correlated positively with cord lead (r=0.905, pË‚0.05), which correlated negatively with head circumference (r=-0.766, pË‚0.05). Birth weight correlated negatively with estradiol (r=-0.295), but positively with testosterone (r=0.006) (pËƒ0.05).
Conclusion: Cord blood estradiol and testosterone levels do not differ between males and females. Estradiol was high in cord blood of PCOS mothers. Testosterone was high in cord blood of PCOS and preeclampsia mothers. The increase in cord lead causes decrease in babies head circumference.
Effects of Eryngium Caucasicum Extract on Testosterone, Inflammation and Oxidative Status of Nicotinamide-Streptozotocin Induced Type-2 Diabetes in Male Rats
Authors: Afshari Masood , Malayeri Ali Reza , Mohammadshahi Majid 
Published: March 2022

Objective: Regarding the unfavorable side effects of anti-diabetic drugs including peripheral edema, gastrointestinal discomfort and hypoglycemia, using medicinal plants due to their useful contents such as flavonoids, alkaloids, glycopeptides, terpenoides, phenolic compounds and other constituents with antioxidant and anti-inflammatory effects, is encouraged in treatment of diabetes mellitus. Thus, the hypothesis was that Eryngium Caucasicum extract will decrease inflammation and oxidative stress in nicotinamide-streptozotocin induced type 2 diabetes mellitus model in Wistar rats.
Methods: In this study, sixty adult male Wistar rats (150 â€“ 250 g) were randomly allocated into six groups (n=10) including: 1: healthy control, 2: diabetic control, 3: diabetic rats which received Sitagliptine, 4, 5 and 6: diabetic rats which received 100, 200 and 300 mg/kg of E. Caucasicum extract oral gavages for 30 days. Eventually, total antioxidant capacity, vitamin B12, malondyaldehyde, interleukin-6, high sensitive C - reactive protein and testosterone serum levels were measured.
Results: Administration of E. Caucasicum in type 2 diabetes mellitus animal model did not change serum vitamin B12 and animal weight compared with control groups. Total antioxidant capacity and malondyaldehyde improved in all doses of E. Caucasicum; in the highest doses, the total antioxidant capacity was higher than sitagliptine group. Interleukin-6 and high sensitive C-reactive protein both decreased in all doses of E. Caucasicum. Administration of E. Caucasicum extract improved testosterone serum level only in highest dose of E. Caucasicum extract. Since the highest dose showed the highest antioxidant and anti-inflammatory effects; dose-responses of antioxidant and anti-inflammatory effects are suggested.
Conclusions: In conclusion, we showed that administration of E. Caucasicum in T2DM animal model has antioxidant and anti-inflammatory effects. However, in future studies other dose-escalating intervention must be performed. Also toxicity in diabetes must be elucidated in future studies.
The efficacy of traditional formulation on quality of life and fatigue in multiple sclerosis patients: a randomized double-blind placebo-control clinical trial
Authors: Namjooyan F. , Ghanavati R. , Majdinasab N. , Rezaei zadeh H 
Published: March 2022

Objective: According to effects that cinnamon, ajwain and Iranian borago shows in involving mechanisms in MS and MS animal model, we decided to survey the affect of traditional formulation on MS patient by clinical trial.
Methods: in a double blind randomized clinical trial study, 60 patients with (MS) observed. They take formulation 15cc per day, fill the MSQOL-54, and fatigue questionnaires every month. The data were analyzed with 18th version of SPSS, independent t-Test and repeated measure tests. The p-value for tests was 0.05.
Results: the mean quality of life and fatigue of patient with MS were significantly changed during 3 month.
Conclusion: most patients with MS had better quality of life during 3 month and get less tired. In some patient tremor and pain reduce. Because of good result and revenue of formulation, it seems to be useful for MS patient.
Nano-encapsulated tarragon (Artemisia dracunculus) essential oil as a sustained release nano-larvicide
Authors: Osanloo Mahmoud , Sedaghat Mohammad Mehdi , Sereshti Hassan , Amani Amir 
Published: March 2022

Objective: In recent years, essential oil-based larvicides have been introduced as alternatives to industrial ones. However, an appreciable formulation of essential oils with prolonged larvicidal activity (LA) has not yet been developed.
Methods: In this study, tarragon essential oil (TEO) was encapsulated in chitosan nanoparticles using ion gelation technique. Physicochemical properties and duration of LA of the prepared nanoformulation were investigated.
Results: Encapsulation efficiency of the optimum nanoformulations with a particle size of 168Â±90 nm was calculated as 39.66% using UV-Vis analysis. Encapsulating TEO in chitosan-TPP nanocapsules was shown to increase its efficiency in LA at SSF test: Perfect LA (100% mortality) was achieved at lower concentration (i.e. 31 Î¼g mL-1 instead of 80 Î¼g mL-1). Also, perfect LA continued for 4 and 3 days, compared with 2 days and 1 day for the non-encapsulated form of TEO in the lab and SFF tests, respectively. Besides, the duration of LA of nanoformulation was significantly longer than its corresponding microformulation with the same concentration of ingredients. Furthermore, the concentration of TEO in the solution tests was also monitored and it was found that the nanoformulations provide a sustained release of TEO. Moreover, there was a logical relationship between LA and concentration of TEO in different hours.
Conclusion: This prepared nanoformulation could be introduced as an interesting alternative to synthetic larvicides, due to its easy and fast method of preparation and its green constituents.
Histological impact of nutritional style alteration in mice
Authors: Salih Khalid M , Jouda Jamela , Asad Shatha Salah , Faraj Yusur Falah , Mohammed Iyden Kamil , Altaee Ali Sameer Abudlghani 
Published: March 2022

Objectives: It is well established that diet and lifestyle are important in maintenance of healthy. Transition from a plant-based diet mostly to a high-calorie diet of animal products might raise the chronic diseases which called â€œdegenerativeâ€. This work aimed to study the histopathological effect of transition from complete plant-based diet to 10% animal products (sheepâ€™s brain) on various body organs of mice.
Methods: Eight-week old Balb/c male mice were divided into 2 groups (n=8); the first is restricted group in which mice were fed on restricted diet containing 10% of sheepâ€™s brain homogenate, while the second is the control group in which fed on ad libitum on the diet for 7 days. During the duration of experiment, body weight and the amount of food intake were recorded daily, then at the end of experiment, all mice were sacrificed and various organs were obtained and processed for histopathological study.
Results: the results showed that food intake by each mouse of restricted group are significantly lower than in control group. Although the mean of body weight in both groups revealed non-significant difference, the relative weight of various organs showed significant differences. On the other hand, sever histological changes were detected in all studied organs sections of restricted group.
Conclusion: It can be concluded that changing in nutritional style rather than conventional diet play a crucial role in modifying the architectural aspects of different organs at tissue level. Therefore, these findings need further investigation at cellular, physiological, and molecular levels.
Predictive factors of survival rate in oral squamous cell carcinoma: A retrospective study in Iran
Authors: Gholizadeh Narges , Emami Razavi Hanieh , Jafari Haftkhani Gholamali , Sheykhbahaei Nafiseh 
Published: March 2022

Objective: Oral cancer as a part of this collection plays an important role in the burden of diseases, especially in South Asia. Despite numerous advances in the treatment of cancers, oral cancer is one of the top ten causes of death due to delayed detection. Given the role of the environmental and climatic conditions in cancers, there is a need for epidemiological studies in different geographic areas.
Methods: In present study, data were collected through medical records of the patients with oral cancer during 2009 to 2012 in the archives of the Cancer Department of the Ministry of Health of the Islamic Republic of Iran. The follow-up of 82 cases was possible in the study. Data collection were performed from questionnaire. The data were analyzed by SPSS version 20. The Kaplan Meier survival curves were utilized and, moreover, the corresponding influential factors were examined by using the Cox regression test.
Result: The overall five-year survival rate was 40.24% (SE= 5.5). The most widely used treatment method was a combination of three methods of surgery, radiation therapy, and chemotherapy. The gender of 64.63%of patients were male. The mean age of patients were 66.1 years. The age and regular periodic follow-up had a statistically significant relation with the survival rate (P=0.02, P=0.03).
Conclusion: Oral cancers require special attention due to high prevalence and overall survival rates. Training oral health care providers, and periodic follow up can be an important step in increasing the survival of these patients.
Breast Cancer Decisive Parameters for Iraqi Women via Data Mining Techniques
Authors: Behadili Suhad Faisal , Abd Mustafa S. , Mohammed Iyden Kamil , Al-SAYYID Maha Mohammed 
Published: March 2022

Objective This research investigates Breast Cancer real data for Iraqi women, these data are acquired manually from several Iraqi Hospitals of early detection for Breast Cancer. Data mining techniques are used to discover the hidden knowledge, unexpected patterns, and new rules from the dataset, which implies a large number of attributes.
Methods Data mining techniques manipulate the redundant or simply irrelevant attributes to discover interesting patterns. However, the dataset is processed via Weka (The Waikato Environment for Knowledge Analysis) platform. The OneR technique is used as a machine learning classifier to evaluate the attribute worthy according to the class value.
Results The evaluation is performed using a training data rather than cross validation. The decision tree algorithm J48 is applied to detect and generate the pattern of attributes, which have the real effect on the class value. Furthermore, the experiments are performed with three machine learning algorithms J48 decision tree, simple logistic, and multilayer perceptron using 10-folds cross validation as a test option, and the percentage of correctly classified instances as a measure to determine the best one from them. As well as, this investigation used the iteration control to check the accuracy gained from the three mentioned above algorithms. Hence, it explores whether the error ratio is decreasing after several iterations of algorithm execution or not.
Conclusion It is noticed that the error ratio of classified instances are decreasing after 5-10 iterations, exactly in the case of multilayer perceptron algorithm rather than simple logistic, and decision tree algorithms. This study realized that the TPS_pre is the most common effective attribute among three main classes of examined dataset. This attribute highly indicates the BC inflammation.
A comparative study of anti-atherosclerotic/anti-inflammatory effect of irbesartan and rosuvastatin in hypercholesterolemic male rabbits
Authors: Majeed Murooj L , Mahdi Hind F , Hadi Najah R , Janabi Ali M 
Published: March 2022

Objective: To evaluate the potential protective effect of irbesartan on atherosclerotic progression using rosuvastatin as a positive control.
Methods: Twenty local domestic rabbits were randomly divided into four groups, five rabbits per each group. The first group (I), rabbits were receiving normal diet; the second group (II), rabbits were receiving 2 % cholesterol diet; the third group (III), rabbits were receiving rosuvastatin and 2% cholesterol diet and; the fourth group (IV), rabbit were receiving irbesartan and 2% cholesterol diet. Serum level of cholesterol, triglycerides, LDL, VLDL and HDL were determined after 12 weeks of the study. Serum level of high sensitive C-reactive protein, ICAM1and VCAM1 were measured after 12 weeks of the study. Total antioxidant activity was also determined. Aortic tissue sent for histopathological examination of atherosclerosis lesion.
Result: Data of this study have showed that atherogenic diet caused an increase in serum level of TC, LDL, VLDL and TG and a decrease in serum level of HDL. Treatment of rabbits with irbesartan caused substantial decrease in serum lipid profile compared with untreated rabbit receiving atherogenic diet. No significant difference was seen between irbesartan- and rosuvastatin-treated rabbits in all lipid parameters measured with the exception that the HDL level in rosuvastatin-treated rabbits was significantly higher than that of irbesartan-treated rabbits. Rabbit treated with irbesartan showed a significant decrease in the serum level of high sensitive C-reactive protein, ICAM1, VCAM1 and aortic total antioxidant capacity when compared with untreated group. No significant difference was seen between irbesartan- and rosuvastatin-treated rabbits in all these inflammatory parameters measured. Histopathological examination showed that irbesartan decreased atherogenic lesion significantly when compared with untreated rabbits.
Conclusion: Irbesartan has a potential protective effect against atherosclerosis via decreasing lipid oxidation and inflammatory mediators. In terms of lipid lowering and anti-inflammatory effects, irebsartan has similar pattern of effectiveness to rosuvastatin.