bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validierung des Fragebogens zum Schlafverhalten von Sportlerinnen und Sportlern (FSVS)
Authors: Berge Annika Hof zum , Kellmann Michael , Loch Fabian , Ferrauti Alexander , Meyer Tim , Pfeiffer Mark , Jakowski Sarah 
Published: December 2022

Zusammenfassung. Der Athlete Sleep Behavior Questionnaire ist ein englischsprachiger Fragebogen zur Erhebung des individuellen Schlafverhaltens von Sportler_innen. Ziel dieser Studie war die Übersetzung des Athlete Sleep Behavior Questionnaire ins Deutsche sowie die wissenschaftliche Validierung dieser deutschen 18-Item-Version, dem Fragebogen zum Schlafverhalten von Sportlerinnen und Sportlern (FSVS). Die Validierungsstichprobe umfasste 450 Teilnehmende. Mittels eines dichotomen Items zur regelmäßigen Wettkampfteilnahme (ja/nein) wurde in Wettkampfsportler_innen ( n = 209) und Kontrollgruppe ( n = 241) unterteilt. Cronbach-α des FSVS-Gesamtwerts ergab .66. Die Pearson-Produkt-Moment-Korrelationsanalysen ergaben moderate Korrelationen zwischen FSVS-Gesamtwert, der Epworth-Sleepiness-Scale ( r = .33) und dem Pittsburgh-Sleep-Quality-Index ( r = .30). Es wurden signifikante Gruppenunterschiede für den FSVS-Gesamtwert gefunden, t‍(448) = 4.47, p < .001, d = 0.42, bei deskriptiv höheren Werten der Gruppe der Wettkampfsportler_innen. In einer separaten Stichprobe, die 13 Ruderer_innen der deutschen Junioren-Nationalmannschaft umfasste, wurden die Fragebogenwerte anschließend mit objektiven Schlafparametern korreliert. Dabei ließ sich eine hohe Korrelation zwischen FSVS-Gesamtwert und Einschlaflatenz ( rs = .68, p = .011) erkennen. Zusammenfassend ist der FSVS ein schnelles und valides Werkzeug, um dysfunktionale Schlafverhaltensweisen bei Wettkampfsportler_innen zu identifizieren. In Anlehnung an die Originalversion wird die Ableitung von Interventionen anhand einzelner Items empfohlen.
Geochemical Characterization of Insoluble Particle Clusters in Ice Cores Using Two‐Dimensional Impurity Imaging
Authors: Bohleber Pascal , Stoll Nicolas , Rittner Martin , Roman Marco , Weikusat Ilka , Barbante Carlo 
Published: December 2022

AbstractUnderstanding post‐depositional processes altering the layer sequence in ice cores is especially needed to avoid misinterpretation of the oldest and most highly thinned layers. The record of soluble and insoluble impurities represents an important part of the paleoclimate proxies in ice cores but is known to be affected through interaction with the ice matrix, diffusion, and chemical reactions. Laser ablation inductively coupled plasma mass spectrometry (LA‐ICP‐MS) has been recognized for its micron‐scale resolution and micro‐destructiveness in ice core impurity analysis. Employing LA‐ICP‐MS for 2D chemical imaging has already revealed a close relationship between the ice grain boundary network and impurity signals with a significant soluble component, such as Na and Mg. Here we show the latest improvements in chemical imaging with LA‐ICP‐MS, by increasing the spatial resolution to 20 μm and extending the simultaneous analysis to also mostly insoluble impurities, such as Al and Fe. All analytes reveal signals of dispersed spots in a sample of an East Greenland ice core. Based on their average size around 50–60 times larger than an average particle and their heterogeneous elemental ratios these spots are interpreted as particle clusters. To distinguish their origin, a simple colocalization classification reveals elemental ratios consistent with marine and mineral dust aerosol. Based on already existing data from cryo‐Raman spectroscopy, we discuss potential ways to integrate the two methods in a future comparison. Such a combined approach may help constraining post‐depositional changes to the dust‐related insoluble impurity components, such as cluster formation and chemical reactions at grain boundaries.
Ganymede MHD Model: Magnetospheric Context for Juno's PJ34 Flyby
Authors: Duling Stefan , Saur Joachim , Clark George , Allegrini Frederic , Greathouse Thomas , Gladstone Randy , Kurth William , Connerney John E. P. , Bagenal Fran , Sulaiman Ali H. 
Published: December 2022

AbstractOn 7 June 2021 the Juno spacecraft visited Ganymede and provided the first in situ observations since Galileo's last flyby in 2000. The measurements obtained along a one‐dimensional trajectory can be brought into global context with the help of three‐dimensional magnetospheric models. Here we apply the magnetohydrodynamic model of Duling et al. (2014, https://doi.org/10.1002/2013ja019554) to conditions during the Juno flyby. In addition to the global distribution of plasma variables we provide mapping of Juno's position along magnetic field lines, Juno's distance from closed field lines and detailed information about the magnetic field's topology. We find that Juno did not enter the closed field line region and that the boundary between open and closed field lines on the surface matches the poleward edges of the observed auroral ovals. To estimate the sensitivity of the model results, we carry out a parameter study with different upstream plasma conditions and other model parameters.
Global Sporadic‐E Occurrence Rate Climatology Using GPS Radio Occultation and Ionosonde Data
Authors: Hodos Travis J. , Nava Omar A. , Dao Eugene V. , Emmons Daniel J. 
Published: December 2022

AbstractAn updated global climatology of blanketing sporadic E (Es) is developed from a combined data set of Global Positioning System (GPS) radio occultation (RO) and ground‐based ionosonde soundings over the period of September 2006–January 2019. A total of 46 sites and 3.2 million total soundings from the Global Ionosphere Radio Observatory network in combination with 3.0 million occultations from the Constellation Observing System for Meteorology, Ionosphere, and Climate constellation are used to calculate global occurrence rates (ORs) for two blanketing frequency thresholds: all blanketing sporadic‐E with no limit on intensity (all‐Es) and moderate‐Eswith fbEs ≥ 3 MHz. Following the GPS‐RO to ionosonde comparison by Carmona et al. (2022),https://doi.org/10.3390/rs14030581the all‐Esrates are calculated using ionosonde data and an amplitude‐based S4threshold for the GPS‐RO data while the moderate‐Esrates use a primarily phase‐based technique. Occurrence rates are separated by intensity, season, month, and solar local time for quiet geomagnetic conditions. Overall, the general geomagnetic trends agree with previous Esclimatologies and the ORs peak near 50% for all‐Esand 25% for moderate‐Esmeasured in the mid‐latitudes during local summer in the late afternoon. Low ORs are observed near the South Atlantic Anomaly and North America, and a general asymmetry is observed between hemispheres with higher ORs in the Northern Hemisphere. High‐latitude and late morning blanketing Esare found to be stronger but less frequent with rates nearly equal to the moderate‐Esmid‐latitude maximums.
Evaluating the Sensitivity of Multi‐Dimensional Model Predictions of Salmon Habitat to the Source of Remotely Sensed River Bathymetry
Authors: Harrison Lee R. , Legleiter Carl J. , Sridharan Vamsi K. , Dudley Peter N. , Daniels Miles E. 
Published: December 2022

AbstractMulti‐dimensional numerical models are fundamental tools for investigating biophysical processes in aquatic ecosystems. Remote sensing techniques increase the feasibility of applying such models at riverscape scales, but tests of model performance on large rivers have been limited. We evaluated the potential to develop two‐dimensional (2D) and three‐dimensional (3D) hydrodynamic models for a 1.6‐km reach of a large gravel‐bed river using three sources of remotely sensed river bathymetry. We estimated depth from hyperspectral image data acquired from conventional and uncrewed aircraft and multispectral satellite imagery. Our results indicated that modeled water depth errors were similar between 2D and 3D models, with depth residuals that were comparable to the uncertainty associated with the bathymetry used as input. We found good agreement between measured and modeled depth‐averaged velocities generated by 2D and 3D models, while 3D models provided superior predictions of near‐bed velocities. We found that optimal model performance occurred for lower flow resistance values than previously reported in the literature, possibly as a consequence of the high‐resolution bathymetry used as model input. Model predictions of winter‐run Chinook salmon (Oncorhynchus tshawytscha) spawning and rearing habitat were not sensitive to the source of bathymetric information, but bioenergetic predictions related to adult holding costs were influenced by the input bathymetry. Our results suggest that hyperspectral imagery acquired from piloted and/or uncrewed aircraft can be used to map the bathymetry of clear‐flowing, relatively shallow large rivers with sufficient accuracy to support multi‐dimensional flow model development; models developed from multispectral satellite imagery had more limited predictive capability.
Compound Coastal, Fluvial, and Pluvial Flooding During Historical Hurricane Events in the Sabine–Neches Estuary, Texas
Authors: Maymandi Nahal , Hummel Michelle A. , Zhang Yu 
Published: December 2022

AbstractWhen storm surge, river discharge, and rainfall occur simultaneously or in succession, they may cause compound flooding and exacerbate impacts in coastal communities. In traditional modeling approaches, these driving processes are simulated separately, leading to discrepancies between simulated and observed data and highlighting the need for coupled modeling frameworks. Here, we develop a coupled coastal–fluvial–pluvial model for the Sabine–Neches Estuary in southeastern Texas using Delft3D to simulate flood extent and depth during Hurricanes Rita, Ike, and Harvey. For each hurricane, four scenarios (i.e., coastal, fluvial, pluvial, and compound) are modeled to determine the relative contribution of each driving force to water levels across the domain. Our results reveal that tropical cyclones such as Harvey that are accompanied by extended periods of rainfall produce the largest extent and longest duration of compound flooding. Such storms also produce highly dynamic and evolving patterns of compound flooding over their duration, with multiple flood peaks that can inhibit response and recovery efforts. When simulating these storms, a coupled model can provide substantial improvements over coastal‐, fluvial‐, and pluvial‐only models, decreasing root mean square error by 69%, 69%, and 79%, respectively. In contrast, storms with short‐duration rainfall and moderate to severe storm surge produce less extensive compound flooding, which is typically dominated by coastal–pluvial interactions. Our results further highlight the potential for compound flooding at the locations of critical water infrastructure assets, suggesting the need for flood adaptation approaches that are robust to multiple types of flooding.
Residual NAPL Morphology Effects on Electrical Resistivity: Insights From Micromodel Displacement Experiments and Pore Network Simulations
Authors: Qiang Siyuan , Shi Xiaoqing , Revil André , Kang Xueyuan , Liu Yuanyuan , Wu Jichun 
Published: December 2022

AbstractCharacterizing residual non‐aqueous phase liquids (NAPLs) in porous media is essential for designing contaminated site remediation strategies. The direct current resistivity method is increasingly being used at sites contaminated by NAPLs, which uses the Archie's second law to connect NAPL saturation with the resistivity of porous media. A power‐law relationship connecting the phase morphology measured by Euler characteristic with resistivity has also been observed at the pore‐scale. Because these relationships are limited for porous media with specific wettability, previous research works have demonstrated deviations from Archie's second law in both the laboratory and field experiments. To evaluate the effects of residual NAPL morphology with different wettability on bulk resistivity, we developed two 2D‐micromodels and measured their electrical resistivity and phase morphology during phase displacement under laboratory‐controlled conditions. Furthermore, pore network simulations of a variety of conditions were performed in absence of surface conduction. Results show that NAPL distributed in dead ends of the pore space has limited influence on bulk resistivity since they do not control the current path and voltage distribution. The correlations between resistivity index and Euler characteristic are determined by NAPL ganglia size and pore throat width. A power‐law relationship is found between the Archie's saturation exponent and the average size of the residual NAPL ganglia, indicating that this exponent can be interpreted as the rate of change of the pore water connectivity with saturation. These insights provide a physical framework connecting the morphology and wettability of the NAPL ganglia with the Archie's saturation exponent.
Drawing a line between histone demethylase KDM5A and KDM5B: their roles in development and tumorigenesis
Authors: Yoo Jung , Kim Go Woon , Jeon Yu Hyun , Kim Ji Yoon , Lee Sang Wu , Kwon So Hee 
Published: December 2022

AbstractDistinct epigenetic modifiers ensure coordinated control over genes that govern a myriad of cellular processes. Growing evidence shows that dynamic regulation of histone methylation is critical for almost all stages of development. Notably, the KDM5 subfamily of histone lysine-specific demethylases plays essential roles in the proper development and differentiation of tissues, and aberrant regulation of KDM5 proteins during development can lead to chronic developmental defects and even cancer. In this review, we adopt a unique perspective regarding the context-dependent roles of KDM5A and KDM5B in development and tumorigenesis. It is well known that these two proteins show a high degree of sequence homology, with overlapping functions. However, we provide deeper insights into their substrate specificity and distinctive function in gene regulation that at times divert from each other. We also highlight both the possibility of targeting KDM5A and KDM5B to improve cancer treatment and the limitations that must be overcome to increase the efficacy of current drugs.
Idarubicin combats abiraterone and enzalutamide resistance in prostate cells via targeting XPA protein
Authors: Zhang Ying , Wei Wei , Li Changying , Yan Siyuan , Wang Shanshan , Xiao Shudong , He Chenchen , Li Jing , Qi Zhi , Li Benyi , Yang Kuo , Li Changlin 
Published: December 2022

AbstractAlthough second-generation therapies like abiraterone (ABI) and enzalutamide (ENZ) benefit patients with castration-resistant prostate cancer (CRPC), drug resistance frequently occurs, eventually resulting in therapy failure. In this study, we used two libraries, FDA-approved drug library and CRISP/Cas9 knockout (GeCKO) library to screen for drugs that overcome treatment resistance and to identify the potential drug-resistant genes involved in treatment resistance. Our screening results showed that the DNA-damaging agent idarubicin (IDA) overcame abiraterone and enzalutamide resistance in prostate cancer cells. IDA treatment inhibited the DNA repair protein XPA expression in a transcription-independent manner. Consistently, XPA knockout sensitized prostate cancer cells to abiraterone and enzalutamide treatment. In conclusion, IDA combats abiraterone and enzalutamide resistance by reducing XPA protein level in prostate cancer.
Non-invasive prenatal testing in Germany: a unique ethical and policy landscape
Authors: Bowman-Smart Hilary , Wiesemann Claudia , Horn Ruth 
Published: December 2022

AbstractNon-invasive prenatal testing (NIPT) has been available commercially in Europe since approximately 2012. Currently, many countries are in the process of integrating NIPT into their publicly funded healthcare systems to screen for chromosomal aneuploidies such as trisomy 21 (Down syndrome), with a variety of implementation models. In 2019, the German Federal Joint Committee (G-BA), which plays a significant role in overseeing healthcare decisions in Germany, recommended that NIPT be reimbursed through public insurance. Following this recommendation, NIPT will be offered on a case-by-case basis, when a pregnant woman, after being counselled, makes an informed decision that the test is necessary in her personal situation. This model differs significantly from many other European countries, where NIPT is being implemented either as a first-tier screening offer available for all pregnancies, or a contingent screen for those with a high probability of foetal aneuploidy (with varying probability cut-offs). In this paper we examine how this unique approach to implementing NIPT in Germany is produced by an ethical and policy landscape resulting from a distinctive cultural and historical context with a significant influence on healthcare decision-making. Due in part to the specific legal and regulatory environment, as well as strong objections from various stakeholders, Germany did not implement NIPT as a first-tier screen. However, as Germany does not currently publicly fund as standard other forms of prenatal aneuploidy screening (such as combined first trimester screening), neither can it be implemented as a screen contingent on specific probability cut-offs. We discuss how German policy reflects the echoes of the past shaping approaches to new biotechnologies, and the implications of this unique model for implementing NIPT in a public healthcare system.
Transient non-Hermitian skin effect
Authors: Gu Zhongming , Gao He , Xue Haoran , Li Jensen , Su Zhongqing , Zhu Jie 
Published: December 2022

AbstractThe discovery of non-Hermitian skin effect (NHSE) has opened an exciting direction for unveiling unusual physics and phenomena in non-Hermitian system. Despite notable theoretical breakthroughs, actual observation of NHSE’s whole evolvement, however, relies mainly on gain medium to provide amplified mode. It typically impedes the development of simple, robust system. Here, we show that a passive system is fully capable of supporting the observation of the complete evolution picture of NHSE, without the need of any gain medium. With a simple lattice model and acoustic ring resonators, we use complex-frequency excitation to create virtual gain effect, and experimentally demonstrate that exact NHSE can persist in a totally passive system during a quasi-stationary stage. This results in the transient NHSE: passive construction of NHSE in a short time window. Despite the general energy decay, the localization character of skin modes can still be clearly witnessed and successfully exploited. Our findings unveil the importance of excitation in realizing NHSE and paves the way towards studying the peculiar features of non-Hermitian physics with diverse passive platforms.
Active eosinophils regulate host defence and immune responses in colitis
Authors: Gurtner Alessandra , Borrelli Costanza , Gonzalez-Perez Ignacio , Bach Karsten , Acar Ilhan E. , Núñez Nicolás G. , Crepaz Daniel , Handler Kristina , Vu Vivian P. , Lafzi Atefeh , Stirm Kristin , Raju Deeksha , Gschwend Julia , Basler Konrad , Schneider Christoph , Slack Emma , Valenta Tomas , Becher Burkhard , Krebs Philippe , Moor Andreas E. , Arnold Isabelle C. 
Published: December 2022

AbstractIn the past decade, single-cell transcriptomics has helped to uncover new cell types and states and led to the construction of a cellular compendium of health and disease. Despite this progress, some difficult-to-sequence cells remain absent from tissue atlases. Eosinophils—elusive granulocytes that are implicated in a plethora of human pathologies1–5—are among these uncharted cell types. The heterogeneity of eosinophils and the gene programs that underpin their pleiotropic functions remain poorly understood. Here we provide a comprehensive single-cell transcriptomic profiling of mouse eosinophils. We identify an active and a basal population of intestinal eosinophils, which differ in their transcriptome, surface proteome and spatial localization. By means of a genome-wide CRISPR inhibition screen and functional assays, we reveal a mechanism by which interleukin-33 (IL-33) and interferon-γ (IFNγ) induce the accumulation of active eosinophils in the inflamed colon. Active eosinophils are endowed with bactericidal and T cell regulatory activity, and express the co-stimulatory molecules CD80 and PD-L1. Notably, active eosinophils are enriched in the lamina propria of a small cohort of patients with inflammatory bowel disease, and are closely associated with CD4+ T cells. Our findings provide insights into the biology of eosinophils and highlight the crucial contribution of this cell type to intestinal homeostasis, immune regulation and host defence. Furthermore, we lay a framework for the characterization of eosinophils in human gastrointestinal diseases.
Early adversity promotes binge-like eating habits by remodeling a leptin-responsive lateral hypothalamus–brainstem pathway
Authors: Shin Sora , You In-Jee , Jeong Minju , Bae Yeeun , Wang Xiao-Yun , Cawley Mikel Leann , Han Abraham , Lim Byung Kook 
Published: December 2022

AbstractEarly-life trauma (ELT) is a risk factor for binge eating and obesity later in life, yet the neural circuits that underlie this association have not been addressed. Here, we show in mice that downregulation of the leptin receptor (Lepr) in the lateral hypothalamus (LH) and its effect on neural activity is crucial in causing ELT-induced binge-like eating and obesity upon high-fat diet exposure. We also found that the increased activity of Lepr-expressing LH (LHLepr) neurons encodes sustained binge-like eating in ELT mice. Inhibition of LHLepr neurons projecting to the ventrolateral periaqueductal gray normalizes these behavioral features of ELT mice. Furthermore, activation of proenkephalin-expressing ventrolateral periaqueductal gray neurons, which receive inhibitory inputs from LHLepr neurons, rescues ELT-induced maladaptive eating habits. Our results identify a circuit pathway that mediates ELT-induced maladaptive eating and may lead to the identification of novel therapeutic targets for binge eating and obesity.
Structural basis for Parkinson’s disease-linked LRRK2’s binding to microtubules
Authors: Snead David M. , Matyszewski Mariusz , Dickey Andrea M. , Lin Yu Xuan , Leschziner Andres E. , Reck-Peterson Samara L. 
Published: December 2022

AbstractLeucine-rich repeat kinase 2 (LRRK2) is one of the most commonly mutated genes in familial Parkinson’s disease (PD). Under some circumstances, LRRK2 co-localizes with microtubules in cells, an association enhanced by PD mutations. We report a cryo-EM structure of the catalytic half of LRRK2, containing its kinase, in a closed conformation, and GTPase domains, bound to microtubules. We also report a structure of the catalytic half of LRRK1, which is closely related to LRRK2 but is not linked to PD. Although LRRK1’s structure is similar to that of LRRK2, we find that LRRK1 does not interact with microtubules. Guided by these structures, we identify amino acids in LRRK2’s GTPase that mediate microtubule binding; mutating them disrupts microtubule binding in vitro and in cells, without affecting LRRK2’s kinase activity. Our results have implications for the design of therapeutic LRRK2 kinase inhibitors.