bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aortic injury repair in a patient with situs inversus totalis and dextrocardia
Authors: Ishimine Tohru , Ishigami Takahiro , Chida Kohei , Taniguchi Naoki , Tengan Toshiho , Mototake Hidemitsu 
Published: March 2022

Abstract
Situs inversus totalis (SIT) with dextrocardia is a rare autosomal recessive disorder. We herein describe a blunt thoracic aortic injury (BTAI) in a patient with SIT and dextrocardia. An 18-year-old girl who was injured by a fall presented to our hospital. Computed tomography (CT) revealed a traumatic pseudoaneurysm at the aortic isthmus. Open aortic repair was performed through a right thoracotomy. No abnormal findings were observed on CT 1 year after the surgery. Open aortic repair of BTAI can be safely performed through a right thoracotomy in patients with SIT and dextrocardia.
Phosphorylated MED1 links transcription recycling and cancer growth
Authors: Chen Zhong , Ye Zhenqing , Soccio Raymond E , Nakadai Tomoyoshi , Hankey William , Zhao Yue , Huang Furong , Yuan Fuwen , Wang Hongyan , Cui Zhifen , Sunkel Benjamin , Wu Dayong , Dzeng Richard K , Thomas-Ahner Jennifer M , Huang Tim H M , Clinton Steven K , Huang Jiaoti , Lazar Mitchell A , Jin Victor X , Roeder Robert G , Wang Qianben 
Published: March 2022

Abstract
Mediator activates RNA polymerase II (Pol II) function during transcription, but it remains unclear whether Mediator is able to travel with Pol II and regulate Pol II transcription beyond the initiation and early elongation steps. By using in vitro and in vivo transcription recycling assays, we find that human Mediator 1 (MED1), when phosphorylated at the mammal-specific threonine 1032 by cyclin-dependent kinase 9 (CDK9), dynamically moves along with Pol II throughout the transcribed genes to drive Pol II recycling after the initial round of transcription. Mechanistically, MED31 mediates the recycling of phosphorylated MED1 and Pol II, enhancing mRNA output during the transcription recycling process. Importantly, MED1 phosphorylation increases during prostate cancer progression to the lethal phase, and pharmacological inhibition of CDK9 decreases prostate tumor growth by decreasing MED1 phosphorylation and Pol II recycling. Our results reveal a novel role of MED1 in Pol II transcription and identify phosphorylated MED1 as a targetable driver of dysregulated Pol II recycling in cancer.
Transient receptor potential melastatin 8 is required for nitroglycerin- and calcitonin gene-related peptide–induced migraine-like pain behaviors in mice
Authors: Wei Chao , Kim Brian , McKemy David D. 
Published: March 2022

Abstract
Migraine is a complex neurovascular disorder that is one of the leading causes of disability and a reduced quality of life. Even with such a high societal impact, our understanding of the cellular and molecular mechanisms that contribute to migraine headaches is limited. To address this complex disorder, several groups have performed genome-wide association studies to elucidate migraine susceptibility genes, with many identifying transient receptor potential melastatin 8 (TRPM8), a cold-sensitive cation channel expressed in peripheral afferents innervating the trigeminovascular system, and the principal mediator of cold and cold pain associated with injury and disease. Interestingly, these migraine-associated single-nucleotide polymorphisms reside in noncoding regions of TRPM8, with those correlated with reduced migraine risk exhibiting lower TRPM8 expression and decreased cold sensitivity. Nonetheless, as a role for TRPM8 in migraine has yet to be defined, we sought to address this gap in our knowledge using mouse genetics and TRPM8 antagonism to determine whether TRPM8 channels or neurons are required for migraine-like pain (mechanical allodynia and facial grimace) in inducible migraine models. Our results show that both evoked and spontaneous pain behaviors are dependent on both TRPM8 channels and neurons, as well as required in both acute and chronic migraine models. Moreover, inhibition of TRPM8 channels prevented acute but not established chronic migraine-like pain. These results are consistent with its association with migraine in genetic analyses and establish that TRPM8 channels are a component of the underlying mechanisms of migraine.
Safety and efficacy of tyrosine kinase inhibitors in pediatric chronic myeloid leukemia: A protocol for systematic review and meta-analysis
Authors: Ata Fateen , Ghasoub Rola S. , Nashwan Abdulqadir J. , Mushtaq Kamran , Aldapt Mahmood B. , Kassem Nancy A. , Chandra Prem , Omar Nabil E. , Yassin Mohamed A. 
Published: March 2022

Abstract

Introduction:
Current evidence regarding the efficacy and safety of tyrosine kinase inhibitors in the pediatrics with chronic myeloid leukemia (CML) is limited. The safety of TKI beyond 15years of use has not been studied even in the adult population. This systematic review and meta-analysis’s main objectives are (a) to combine the existing knowledge of efficacy and safety profile of TKI in pediatric CML and (b) to compare the side effects of long-term TKI therapy in the pediatric population versus stem cell transplantation.

Methods:
A systematic review with meta-analysis will be performed. Multiple online databases will be explored via pre-decided searching strategy to find eligible studies. All studies reporting on the efficacy and safety of TKI in pediatrics. We will include case reports and series, retrospective and prospective observational studies, studies with experimental designs and gray literature including abstracts from various hematology conferences. The quality of articles will be analyzed by 2 independent reviewers following Reporting Guidelines for Meta-analyses of Observational Studies and Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. CMA and STATA will be utilized to perform the meta-analysis.

Discussion:
This study will be among the initial ones to explore and combine the evidence available on the safety and efficacy of TKI in pediatric CML patients in the form of systematic review and meta-analysis. By acquiring and summarizing data about the use of TKI therapy in pediatric CML patients, the results from this study will give insights for future prospective studies and will provide a better understanding of the efficacy and safety of TKI in pediatric CML.

PROSPERO Registration:
(CRD42020154435).
Neutrophil–lymphocyte ratio and the risk of 30-day mortality in patients with overt hepatic encephalopathy
Authors: Shi Ke , Huang Yunyi , Zhang Qun , Li Yuxin , Wang Xianbo 
Published: March 2022

Aim
Patients with overt hepatic encephalopathy (OHE) have an increased risk of adverse outcomes. However, the relationship between neutrophil to lymphocyte ratio (NLR) and the 30-day risk of death in patients with OHE has not been well evaluated.

Methods
We retrospectively analyzed 1301 patients with OHE at Beijing Ditan Hospital between August 2008 and December 2018. After adjustment for major risk factors, Cox regression analysis and restricted cubic splines were used to analyze the relation between NLR and 30-day mortality. The 30-day survival was calculated by Kaplan–Meier method.

Results
All patients were divided into four subgroups on the basis of the quartiles of the baseline NLR distribution (< 2.5, 2.5–4.3, 4.3–7.5, >7.5). The 30-day mortality rates were 7.8%, 12.7%, 19.5% and 34.1%, respectively (P < 0.001). Compared with the lowest quartile, the increase in the NLR was associated with an increase risk of 30-day mortality after multivariable adjustment (NLR 2.5–4.3: adjusted hazard ratio [AHR], 1.17 (95% confidence interval [CI], 0.70–1.95); NLR 4.3–7.5: AHR, 1.58 (95% CI, 1.01–2.47); NLR > 7.5: AHR, 2.32 (95% CI, 1.50–3.57). A nonlinear association between NLR and the adjusted probability of 30-day mortality was observed. Elevated NLR was correlated with increased 30-day mortality in patients with OHE across different subgroups (HR >1.0).

Conclusion
An elevated NLR is independently associated with a higher risk of 30-day mortality in patients with OHE.
Emergency Medical Services Provider Acceptance of and Attitudes About Pediatric SimBox Simulations
Authors: Cicero Mark Xavier , Baird Janette , Adelgais Kathleen , Brown Linda , Auerbach Marc 
Published: March 2022

Objective
SimBox simulations allow for high-frequency open-access health care education, overcoming cost and resource barriers. Prehospital paramedics and emergency medical technician (EMT) care for children infrequently. In this study, prehospital providers evaluated pediatric SimBox simulations.

Methods
This was a cross-sectional study of EMS professionals participating in a series of simulations conducted in a larger project assessing improvement of the quality of pediatric care in the prehospital setting. Participants were teams of two, which comprised a paramedic/paramedic, paramedic/EMT, or 2 EMTs. The simulations used facilitator resources, debriefing prompts, video depictions of patients and vital signs, and a low-fidelity manikin. Pediatric emergency care coordinators, EMS training officers, and/or emergency physicians facilitated simulations of seizure, sepsis with respiratory failure, and child abuse, followed by debriefings. Participants completed an online survey after the simulation and rated it in 4 domains: prebriefing, scenario content, debriefing, and overall. Ratings were trifold: “strongly agree,” “somewhat agree,” or “do not agree.” Data were analyzed by case type, participant type, location, participant reaction to simulation elements, and the debriefing. Net Promoter Scores were calculated to assess participant endorsement of SimBox.

Results
There were 121 participants: 103 (87%) were paramedics, and 18 (13%) were EMTs. Participant agreement of simulation benefit for clinical practice was high, for example, “I am more confident in my ability to prioritize care and interventions” (98.4% strongly or somewhat agree), and 99.2% of participants agreed the postsimulation debriefing with facilitators “provided opportunities to self-reflect on my performance during simulation.” Overall, 97.5% strongly or somewhat agreed that the simulations “improved my comfort in pediatric acute care.” Net Promoter Score showed 65.3% were promoters of and 24% were passive about SimBox.

Conclusion
SimBox simulations are associated with improved self-efficacy of prehospital care providers for care of acutely ill or injured children. The majority promotes SimBox as a learning tool.
FDG PET/MRI in Synchronous Uterine Adenocarcinoma and Leiomyosarcoma
Authors: Yokoo Shiho , Zeng Feibi , Nogami Munenobu , Ueno Yoshiko R. , Murakami Takamichi 
Published: March 2022

Abstract
A 52-year-old woman with a rapidly growing uterine tumor suspected of uterine sarcoma underwent 18F-FDG PET/MRI, revealing a myometrial mass and an endometrial lesion, suggesting dual primary neoplasms. Based on the PET/MRI findings, we changed the intraoperative procedure to determine the necessity of pelvic lymphadenectomy. PET/MRI was useful in diagnosing and differentiating between 2 malignant neoplasms in the uterus compared with PET/CT, due to MRI's high contrast resolution and precise fusion due to the simultaneous acquisition.
A Peculiar 18F-FDG Spinal Uptake Helps Diagnosis and Effective Treatment of Polymyalgia Rheumatica
Authors: Lanzafame Helena , Zanoni Lucia , Allegri Vincenzo , Mulè Rita , Fanti Stefano 
Published: March 2022

Abstract
Polymyalgia rheumatica is the most common inflammatory rheumatic disease in elderly people, usually develops in patients older than 50 years, more frequently in females. An emerging imaging tool in the diagnostic workup of this condition is whole-body PET/CT, which allows an overall assessment of the articular and extra-articular structures involved.
Acinetobacter baumannii sampled from cattle and pigs represent novel clones
Authors: Mateo-Estrada Valeria , Vali Leila , Hamouda Ahmed , Evans Benjamin A. , Castillo-Ramírez Santiago 
Published: March 2022

AbstractAcinetobacter baumannii is a very important human pathogen. Nonetheless, we know very little about non-human isolates of A. baumannii. Here we determine the genomic identity of 15 cattle and pig isolates, as well their antibiotic and virulence genetic determinants, and compare them to the main human clinical international clones.
Characterization of the growth and morphology of a BSL-2 Coccidioides posadasii strain that persists in the parasitic life cycle at ambient CO2
Authors: Garcia Javier A. , Vu Kiem , Thompson George R. , Gelli Angie 
Published: March 2022

AbstractCoccidioides is a dimorphic fungus responsible for Valley Fever and is the cause of severe morbidity and mortality in the infected population. Although there is some insight into the genes, pathways, and growth media involved in the parasitic to saprophytic growth transition, the exact determinants that govern this switch are largely unknown. In this work, we examined the growth and morphology of a novel Coccidioides posadasii strain (C. posadasii S/E) that efficiently produces spherules and endospores and persists in the parasitic life cycle at ambient CO2. We demonstrated that C. posadasii S/E remains virulent in an insect infection model. Surprisingly, under spherule-inducing conditions, C. posadasii S/E culture was found to be completely hyphal. Differential interference contrast (DIC) and transmission electron microscopy (TEM) revealed unexpected cellular changes in this strain including cell wall remodeling and formation of septal pores with Woronin bodies. Our study suggests that the C. posadasii S/E strain is a useful BSL-2 model for studying the molecular factors that are active during the parasitic life cycle and the mechanisms underlying the parasitic to saprophytic growth transition – a morphological switch that can impact the pathogenicity of the organism in the host.
Accounting for cis-regulatory constraint prioritizes genes likely to affect species-specific traits
Authors: Starr Alexander L. , Gokhman David , Fraser Hunter B. 
Published: March 2022

AbstractMeasuring allele-specific expression in interspecies hybrids is a powerful way to detect cis-regulatory changes underlying adaptation. However, it remains difficult to identify genes most likely to explain species-specific traits. Here, we outline a simple strategy that leverages population-scale allele-specific RNA-seq data to identify genes that have constrained cis- regulation within species yet show divergence between species. Applying this strategy to data from human-chimpanzee hybrid cortical spheroids, we identify signatures of lineage-specific selection on genes related to cellular proliferation, speech, and glucose metabolism. We also highlight cis-regulatory divergence in CUX1 and EDNRB that may shape the unique trajectory of human brain development.
Impact of innovativeness, risk-taking, and proactiveness on export performance in a developing country: evidence of qualitative study
Authors: Hossain Kamal , Soon Lee Kenny Cheah , Abdul Ghani Azmi Ilhaamie Binti , Idris Aida Binti , Alam Mohammad Nurul , Rahman Md. Adnan , Mohd Ali Norinah 
Published: March 2022

Purpose
This study aims to explain the effect of entrepreneurial orientation (EO) dimensions on firms’ export performance. The study has considered three dimensions of EO: innovativeness, proactiveness and risk-taking. Export performance has been measured through multifaceted determinants: financial, strategic and satisfaction levels of exporting firms.

Design/methodology/approach
To explain the association between such determinants, qualitative face-to-face, in-depth interviews were conducted with the respondents from the apparel manufacturing companies of Bangladesh. The study has also applied a content analysis technique to explore and triangulate the descriptions of EO dimensions on performance.

Findings
The research results reveal that EO has a meaningful explanation of export performance from the lived experience of the respondents. The study has also found frequent explanations and indicators about the association of innovativeness and proactiveness on export performance, but the risk-taking dimension has revealed no effects.

Research limitations/implications
The research has different implications on theoretical, managerial and policy-making aspects of EO and export performance. Nevertheless, the findings are based on the opinions of a small sample of entrepreneurs and high-level managers. Thus, it is important to test the generalizability of these findings in future research using a larger sample.

Originality/value
This study indicated product, process and market creation innovativeness. Risks related to product innovation, new market entry and politics are considered for risk-taking measures, while proactive innovation, technology and new market searching are used for measuring proactiveness. Additionally, multifaceted performance determinants have been applied to evaluate export performance. Hence, this study is a unique qualitative analysis with important theoretical, practical and methodological implications.
In-situ adaptive thermal simulation method for predicting and avoiding hotspots in material extrusion processes
Authors: Driezen Jorne , Herrmann Axel S. 
Published: March 2022

Purpose
This study aims to design a novel strategy to avoid thermal defects in three-dimensional (3D) printing processes. A combination of subroutines and utility routines allows for in situ variations of the key process parameters (KPP) in the thermal simulation and produces by post-processing the output an updated machine code for enhanced quality of the printed part in a single iteration.

Design/methodology/approach
The input data for the thermal simulation were obtained by characterising an acrylonitrile butadiene styrene filament and calibrating an Ultimaker S5 printer. Abaqus subroutines were used to accurately simulate the continuous deposition of molten material. A utility routine extracted the nodal temperatures during simulation and detected regions that were prone to overheating. We developed a method, enabling the introduction of variable process parameters in the thermal simulation and validated it by printing several test geometries.

Findings
The results of the thermal simulation indicate that decreasing the print speed enhances the amount of heat dissipated to the environment. Therefore, it is an efficient way to avoid overheating of the printed geometry. The validation geometries showed improvements when adapting the print speed.

Originality/value
This approach is the first to demonstrate the potential of variable process parameters using implemented routines in the thermal process simulation. Here, the focus is on predicting and avoiding thermal hotspots. However, more potential can be obtained by enabling the prediction and adaptation of KPP to ensure mechanical performance. This would be an alternative way to tackle the cost- and time-inefficient post-processing of 3D-printed parts.
The role of social media in the political involvement of millennials
Authors: Hamid Rahmad Solling , Abror Abror , Anwar Suhardi M. , Hartati Andi 
Published: March 2022

Purpose
This study aims to examine the relationship of information quality of social media, social media reputation, social media political marketing activities, trust and political involvement of millennials.

Methodology
The empirical analysis was conducted using a sample of 309 millennials. This study used online survey for the data collection. After passing reliability and validity tests, the data were analyzed with partial least squares structural equation modeling.

Findings
The results show that information quality of social media has positive and significant direct influence on reputation and trust. Information quality of social media also has a significant indirect influence on trust through social media reputation. However, there is no significant relationship between information quality and political involvement. Social media political marketing activities also have a direct and indirect significant effect on political involvement through trust. Finally, trust also has a positive and significant impact on political involvement.

Practical implications
This research may contribute to the political marketing experts and politicians in increasing the quality and credibility of advertisements on social media, which will affect trust and political involvement of millennial generation. Moreover, politicians and political marketing experts who have an online-based community should optimize their marketing activities in social media to encourage positive behavior and trust from social media users.

Value
This study has shown a more comprehensive model of the relationship between information quality of social media and political involvement. This study also reveals the significant indirect effect of the trust on the relationship between information quality on social media, social media political marketing activities and political involvement.
Lower efficacy of omalizumab in older adults with chronic spontaneous urticaria
Authors: Kitao Rikuma , Oda Yoshiko , Washio Ken , Tai Yukimasa , Ono Ryusuke , Nishigori Chikako , Fukunaga Atsushi 
Published: March 2022

AbstractOmalizumab is known to be effective in treating chronic spontaneous urticaria (CSU) with an inadequate response to H1‐antihistamine. Although many reports have described pre‐treatment biomarkers to predict the efficacy of omalizumab in CSU, there are few reports that examined the relationship between age and the therapeutic effectiveness of omalizumab. Thus, we aimed to investigate the relationship between response to omalizumab and age. This retrospective study comprised 52 CSU patients receiving three consecutive omalizumab courses during the period from April 2017 to March 2021. Participants were categorized as responders or non/partial responders using the urticaria control test to evaluate clinical variables on week 12. The female rate tended to be higher, and the mean age and the median disease duration tended to be lower with no significance in responders compared with in non/partial responders. In addition, they exhibited no significant differences regarding serum immunoglobulin E levels, basophil counts, eosinophil counts, d‐dimer, and autologous serum skin test results reported as predictor in the past between two groups. Interestingly, when patients were categorized as age <65 years or ≥65 years, those in the ≥65 years group had a significantly lower response to omalizumab than those aged <65 years. These findings suggest that physicians should keep in mind that the age of their CSU patients may be a predictor of the therapeutic efficacy of omalizumab.