bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting weight loss and psychological well‐being in patients with obesity: A sequential combination of behavioural lifestyle intervention and well‐being therapy
Authors: Zhu Boheng , Gostoli Sara , Benasi Giada , Patierno Chiara , Petroni Maria Letizia , Nuccitelli Chiara , Marchesini Giulio , Fava Giovanni A. , Rafanelli Chiara 
Published: November 2022

AbstractBehavioural lifestyle interventions focused on diet and physical activity are a cornerstone for the treatment of obesity. However, their effects vary substantially across individuals in terms of magnitude and durability. Personalized approaches that target psychological well‐being may be promising to facilitate healthy behaviours and sustained weight loss. This preliminary study aimed to explore whether the sequential combination of behavioural lifestyle intervention (BLI) and well‐being therapy (WBT) may result in more favourable outcomes than BLI alone in promoting weight loss (primary outcome) and improving psychological well‐being, distress, dietary behaviours and physical activity (secondary outcomes). A total of 83 patients with obesity were randomly assigned to BLI/WBT (N = 38) or BLI group (N = 45). The BLI group received a 12‐week behavioural weight loss programme, whereas the BLI/WBT group received the same programme followed by an additional 4‐week WBT, adapted for group interventions. Data were collected at pretreatment (baseline, T1), at the end of BLI/WBT (T2), at 6‐month (T3) and 12‐month (T4) follow‐ups. There was a significant weight loss in both treatment groups at T2, T3 and T4. The BLI/WBT group showed greater improvements in depressive symptoms at T3 and T4, in autonomy at T2, in personal growth at T4 and in global well‐being at T4 compared with BLI group. WBT yielded no additional effect on weight loss. However, the secondary outcomes indicate that WBT may have enduring effects that reduce vulnerability to psychological distress in patients with obesity. In order to confirm these preliminary findings and explore whether a more intensive and individualized WBT can foster sustained weight loss, future studies are needed.
Quality Control Procedures for Genome‐Wide Association Studies
Authors: Truong Van Q. , Woerner Jakob A. , Cherlin Tess A. , Bradford Yuki , Lucas Anastasia M. , Okeh Chelsea C. , Shivakumar Manu K. , Hui Daniel H. , Kumar Rachit , Pividori Milton , Jones S. Chris , Bossa Abigail C. , Turner Stephen D. , Ritchie Marylyn D. , Verma Shefali S. 
Published: November 2022

AbstractGenome‐wide association studies (GWAS) are being conducted at an unprecedented rate in population‐based cohorts and have increased our understanding of the pathophysiology of many complex diseases. Regardless of the context, the practical utility of this information ultimately depends upon the quality of the data used for statistical analyses. Quality control (QC) procedures for GWAS are constantly evolving. Here, we enumerate some of the challenges in QC of genotyped GWAS data and describe the approaches involving genotype imputation of a sample dataset along with post‐imputation quality assurance, thereby minimizing potential bias and error in GWAS results. We discuss common issues associated with QC of the GWAS data (genotyped and imputed), including data file formats, software packages for data manipulation and analysis, sex chromosome anomalies, sample identity, sample relatedness, population substructure, batch effects, and marker quality. We provide detailed guidelines along with a sample dataset to suggest current best practices and discuss areas of ongoing and future research. © 2022 Wiley Periodicals LLC.
Small Interfering RNA Delivery Into Primary Human Natural Killer Cells for Functional Gene Analyses
Authors: Momayyezi Pouria , Malmberg Karl‐Johan , Hammer Quirin 
Published: November 2022

AbstractStudying gene functions in human natural killer (NK) cells is key for advancing the understanding of NK cell biology and holds promise to pave the way for improving NK cell therapies against cancer. However, NK cells are challenging to manipulate, and investigation of gene functions in NK cells is hampered by variable delivery efficiencies and impaired viability upon electroporation, lipofection, or viral transduction. Here, we report a simple workflow for delivery of commercially available small interfering RNA molecules into primary human NK cells to enable functional gene analyses. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Enrichment of natural killer cells from human peripheral blood mononuclear cellsBasic Protocol 2: Preparation of small interfering RNABasic Protocol 3: Delivery of small interfering RNA into natural killer cellsSupport Protocol 1: Isolation of human peripheral blood mononuclear cells from buffy coatsSupport Protocol 2: Thawing and recovery of cryopreserved peripheral blood mononuclear cellsSupport Protocol 3: Evaluation of natural killer cell purity following magnetic enrichmentSupport Protocol 4: Exemplary assessment of knockdown efficiency
Mechanochemistry Milling of Waste Poly(Ethylene Terephthalate) into Metal–Organic Frameworks
Authors: He Panpan , Hu Zhen , Dai Zhikun , Bai Huiying , Fan Zifen , Niu Ran , Gong Jiang , Zhao Qiang , Tang Tao 
Published: November 2022

AbstractConverting poly(ethylene terephthalate) (PET) into metal–organic frameworks (MOFs) has emerged as a promising innovation for upcycling of waste plastics. However, previous solvothermal methods suffer from toxic solvent consumption, long reaction time, high pressure, and high temperature. Herein, a mechanochemical milling strategy was reported to transform waste PET into a series of MOFs with high yields. This strategy had the merits of solvent‐free conditions, ambient reaction temperature, short running time, and easy scale‐up for large‐scale production of MOFs. The as‐prepared MOFs exhibited definite crystal structure and porous morphology composed of agglomerated nanoparticles. It was proven that, under mechanochemical milling, PET was firstly decomposed into 1,4‐benzenedicarboxylate, which acted as linkers to coordinate with metal ions for forming fragments, followed by the gradual arrangement of fragments into MOFs. This work not only promotes high value‐added conversion of waste polyesters but also offers a new opportunity to produce MOFs in a green and scalable manner.
Modeling and implementation of a novel active voltage balancing circuit using deep recurrent neural network with dropout regularization
Authors: Noohi Mostafa , Faraji Amin , Sadrossadat Sayed Alireza , Mirvakili Ali , Moftakharzadeh Ali 
Published: November 2022

SummaryRecurrent neural networks (RNN) emerged as powerful tools to model and analyze the nonlinear behavior of electronic circuits accurately and quickly. Efforts to improve the accuracy of RNN will lead to the design of better‐quality products, which is essential in various fields such as the design of energy harvesting (EH) systems. EH techniques can provide the electrical energy needed for low‐power electronics without the need for a battery or with minimal dependency. Due to the importance of the active voltage balancing circuit in EH systems, we have proposed a new macromodeling method called dropout local‐feedback deep recurrent neural network (Dropout‐LFDRNN) to model and analyze this circuit along with two other nonlinear circuits as examples. This technique is an advance over the LFDRNN macromodeling method, and based on the obtained results from the measurements, we were able to build a fast macromodel for the active balancing circuit, which outperforms conventional deep recurrent neural network modeling method in terms of accuracy without sacrificing speed. It is worth mentioning that this proposed technique in this paper can be considered a viable approach for modeling and analysis of nonlinear electronic components and circuits. In addition to the advantage of generating a more accurate model, the model based on the Dropout‐LFDRNN approach is much faster than the existing transistor‐level models.
A case of breast cancer with extensive colon metastasis
Authors: Imai Jin , Hanamura Toru , Kawanishi Aya , Ueda Takashi , Mishima Yusuke , Ito Ayano , Shirataki Yoshihiro , Morimachi Masashi , Kodama Toshio , Sato Hirohiko , Kaneko Motoki , Sano Masaya , Teramura Erika , Monma Makiko , Tsuda Shingo , Tsuruya Kota , Mizukami Hajime , Arase Yoshitaka , Fujisawa Mia , Miyahara Saya , Nakamura Naoya , Suzuki Takayoshi , Matsushima Masashi , Suzuki Hidekazu , Takashimizu Shinji , Kagawa Tatehiro , Nishizaki Yasuhiro 
Published: November 2022

AbstractBreast cancer is one of the most common malignancies in women worldwide. Although most breast cancers are curable, in cases of metastasis, many are often found in the lungs, bones, liver, and central nervous system; however, metastasis to the gastrointestinal tract is rare. Invasive lobular carcinoma, which represents only 5%–10% of breast cancers, has a higher risk of metastasis to the gastrointestinal tract than invasive ductal carcinoma. Here, we report a rare case of gastrointestinal metastasis of invasive lobular carcinoma that spread extensively to the colonic mucosa. Given the improved survival rates of breast cancer patients with current treatments, many rarer metastatic diseases, including gastrointestinal metastases, are likely to be increased in the future.
Failure modes analysis and reliability enhancement of wind turbines
Authors: Abdelmoumene Abdelkader , Bentarzi Hamid 
Published: November 2022

AbstractThe transition of the global energy system to affordable, reliable and clean sources is an important target of several international climate change accords and conferences such as the United Nations Framework Convention on Climate Change (UNFCCC) and its Conferences of parties (COP), Kyoto protocol and the UN Sustainable Development Goals (SDGs). The wind power is at the heart of these energy systems transformation and climate change mitigation. Indeed, the global capacity of wind turbines has seen tremendous growth, especially, during the past two decades which has made wind energy the fastest growing renewable source in the world. Nevertheless, the problem of premature failures and low‐reliability level of wind turbines have been increasingly noticed. This paper presents a new systematic analytical approach for failure modes and reliability analysis of wind turbines. The approach is a combination of three aspects which are qualitative and quantitative analysis using the fault tree method, reliability assessment as well as lifetime estimation and enhancement. Firstly, the various failure modes are presented. Then, mathematical modeling and reliability evaluation have been developed and simulated. Afterward, the actions that can be considered to improve the reliability and, consequently, the lifetime of wind turbines have been presented and analyzed. The results of the developed approach revealed that major reliability improvements, failure rate reduction and lifetime extension can be achieved, leading to a better return on investment, reduced maintenance as well as a safer and more reliable clean energy production.
Altered glial expression of the cannabinoid 1 receptor in the subiculum of a mouse model of Alzheimer's disease
Authors: Terradillos Itziar , Bonilla‐Del Río Itziar , Puente Nagore , Serrano Maitane , Mimenza Amaia , Lekunberri Leire , Anaut‐Lusar Ilazki , Reguero Leire , Gerrikagoitia Inmaculada , Ruiz de Martín Esteban Samuel , Hillard Cecilia J. , Grande María T. , Romero Julián , Elezgarai Izaskun , Grandes Pedro 
Published: November 2022

AbstractThe alteration of the endocannabinoid tone usually associates with changes in the expression and/or function of the cannabinoid CB1 receptor. In Alzheimer's disease (AD), amyloid beta (Aβ)‐containing aggregates induce a chronic inflammatory response leading to reactivity of both microglia and astrocytes. However, how this glial response impacts on the glial CB1 receptor expression in the subiculum of a mouse model of AD, a brain region particularly affected by large accumulation of plaques and concomitant subcellular changes in microglia and astrocytes, is unknown. The CB1 receptor localization in both glial cells was investigated in the subiculum of male 5xFAD/CB2EGFP/f/f (AD model) and CB2EGFP/f/f mice by immuno‐electron microscopy. The findings revealed that glial CB1 receptors suffer remarkable changes in the AD mouse. Thus, CB1 receptor expression increases in reactive microglia in 5xFAD/CB2EGFP/f/f, but remains constant in astrocytes with CB1 receptor labeling rising proportionally to the perimeter of the reactive astrocytes. Not least, the CB1 receptor localization in microglial processes in the subiculum of controls and closely surrounding amyloid plaques and dystrophic neurites of the AD model, supports previous suggestions of the presence of the CB1 receptor in microglia. These findings on the correlation between glial reactivity and the CB1 receptor expression in microglial cells and astrocytes, contribute to the understanding of the role of the endocannabinoid system in the pathophysiology of Alzheimer's disease.
Toward a tiered regulatory framework for the prospective aquatic effect assessment of pesticides in (Neo)tropical areas
Authors: Daam Michiel A. 
Published: November 2022

Abstract
Research and regulatory interest into (Neo)tropical aquatic pesticide ecotoxicology has increased considerably over the past few years. However, pesticide effect assessment frameworks in (Neo)tropical areas remain largely undeveloped and as such not implemented. The present study provides an overview of studies into the comparison of pesticide sensitivity between (Neo)tropical and temperate aquatic taxa. Recommended assessment factors to be applied to temperate toxicity data for use in tropical effect assessments differ significantly between these studies. Shortcomings and bottlenecks of available pesticide sensitivity comparison studies based on results from bioassays, species sensitivity distributions, and model ecosystem studies are scrutinized. Current data lacks and (subsequent) indications for future research are also covered. Ultimately, implications for procedures aimed at the derivation of regulatory acceptable concentrations for pesticides in (Neo)tropical edge-of-field surface waters are discussed. Integr Environ Assess Manag 2023;19:638–647. © 2022 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Synthesis, photophysical properties, and DFT calculation of yellow‐red phosphorescent iridium(III) complexes based on thiophen‐pyrimidine/pyridine derivatives
Authors: Zhang Xiao‐Bin , Tong Wan‐Yue , Chen Meng‐Sen , Xie Jian‐Li , Wang Yi‐Tong , Mo Zheng‐Rong , Wu Shui‐Xing , Niu Zhi‐Gang , Li Gao‐Nan 
Published: November 2022

AbstractA series of heteroleptic phosphorescent iridium(III) complexes [Ir(C^N)2(O^O)] (C^N = 2‐(thiophen‐2‐yl)pyrimidine (tppm), 2‐(benzo[b]thiophen‐2‐yl)pyrimidine (btppm), 2‐(thiophen‐2‐yl)‐4‐(trifluoromethyl)pyridine (cf3thp), 2‐(benzo[b]thiophen‐2‐yl)‐4‐(trifluoromethyl)pyridine (cf3btp); O^O = acetylacetonate (acacH)) have been synthesized and characterized, and their photophysical properties are reported. All of these complexes emit yellow‐red color in the spectral range of 558–647 nm in CH2Cl2 solution at room temperature with quantum yields of 20.1–50.9% and emission lifetimes of 0.23–0.33 μs. Density functional theory (DFT) and time‐dependent‐DFT (TD‐DFT) calculations on these complexes facilitate a detailed assignment of the excited states involved in the absorption and emission processes, that is, metal‐to‐ligand and ligand‐centered charge‐transfer (MLCT/LC) transitions. These charge‐transfer‐type bands are dominated by C^N ligands more than O^O ligand.
Association between preadmission low‐density lipoprotein cholesterol concentration and risk of large intracerebral hemorrhage: Results from the Kailuan study
Authors: Li Zhanying , Hui Ying , Sha Zhuang , Liu Bailu , Wang Chengbo , Yang Feng , Zhang Wenfei , Gao Chuang , Jiang Rongcai 
Published: November 2022

AbstractBackgroundTo examine the association between low‐density lipoprotein cholesterol (LDL‐C) concentrations and the risk of a large hematoma volume after intracerebral hemorrhage (ICH).MethodsPatients from the Kailuan study (Tangshan, China) who were hospitalized with ICH during 2006 and 2020 were included in this study. The concentration of lipid concentrations, hematoma volume and other clinical characteristics were retrospectively collected and analyzed. Hematoma volumes were measured on the first available brain scan using the ABC/2 method. LDL‐C concentrations were obtained from the last physical examination before the occurrence of ICH. LDL‐C concentration was categorized into four groups in accordance with the quartiles. Logistic regression was used to assess the association between LDL‐C concentrations and the risk of a large hematoma volume of ≥30 ml. A generalized linear regression model was used to analyze the dose–response relationship between LDL‐C concentration and hematoma volume.ResultsA total of 836 patients with ICH were evaluated. In the Multivariate logistic regression, compared to the second quartile of LDL_C, the first quartile of LDL_C had a significantly higher risk of a large hematoma volume (OR 2.49 [95% CI 1.54–4.01]), and the higher quartile of LDL_C is not associated with higher odds of large hematoma volume. In the generalized linear regression model, the adjusted β for the association between LDL‐C concentration and hematoma volume was 9.46 (95% confidence interval 2.87–16.04), whereas higher LDL‐C concentration was not associated with a large hematoma volume.ConclusionsThis study confirmed that low LDL‐C concentrations prior to ICH are associated with a higher risk of a large hematoma volume.
Effects of six kinds of sperm staining methods on human sperm size and evaluation of their staining effects
Authors: Xu Yuan‐Hua , Lu Jin‐Chun , Tang Shan‐Shan 
Published: November 2022

AbstractBackgroundLarge‐ and small‐headed sperm are common morphological abnormalities. If different sperm staining methods affect sperm size, they will make a difference in the accuracy of sperm morphological analysis results. In this case, the normal reference values of sperm head parameters for different staining methods should be established.MethodsSix sperm staining methods, including Papanicolaou, Diff‐Quik, Shorr, Hematoxylin–eosin (HE), Wright, and Wright‐Giemsa staining, were used to stain the sperm smears of 25 semen samples, respectively. Sperm head parameter's length (L), width (W), area (A), perimeter, acrosomal area (Ac), and the derived values L/W and Ac/A of 2500 sperm (100 for each specimen) per staining method were measured by a computer‐aided sperm morphological analysis system.ResultsThe highest sperm head length and width were observed with the Wright‐Giemsa and Wright staining, followed by the Diff‐Quik. The lowest sperm head length and width were observed with the Papanicolaou staining, and the sperm head length and width of HE and Shorr staining were between those of Papanicolaou and Diff‐Quik staining. There was the same trend in changes in sperm head area and perimeter. Diff‐Quik and Shorr staining could clearly distinguish acrosome and nucleus, followed by HE staining, whereas the boundary between acrosome and nucleus was not evident in Papanicolaou, Wright, and Wright‐Giemsa staining.ConclusionDifferent staining methods influence sperm size, and the normal reference values of sperm head parameters of each staining method should be established. Diff‐Quik and Shorr staining may be suitable methods for routine sperm morphological analysis.
Association of CNR1 gene and cannabinoid 1 receptor protein in the human brain
Authors: Pak Kyoungjune , Kantonen Tatu , Pekkarinen Laura , Nuutila Pirjo , Nummenmaa Lauri 
Published: November 2022

AbstractWe aimed to integrate genomic mapping from brain mRNA atlas with the protein expression from positron emission tomography (PET) scans of type 1 cannabinoid (CB1) receptor and to compare the predictive power of CB1 receptor with those of other neuroreceptor/transporters using a meta‐analysis. Volume of distribution (VT) from F18‐FMPEP‐d2 PET scans, CNR1 gene (Cannabinoid receptor 1) expression, and H3‐CP55940 binding were calculated and correlation analysis was performed. Between VT of F18‐FMPEP‐d2 PET scans and CNR1 mRNA expression, moderate strength of correlation was observed (rho = .5067, p = .0337). Strong positive correlation was also found between CNR1 mRNA expression and H3‐CP55940 binding (r = .6336, p = .0364), validating the finding between F18‐FMPEP‐d2 PET scans and CNR1 mRNA. The correlation between VT of F18‐FMPEP‐d2 PET scans and H3‐CP55940 binding was marginally significant (r = .5025, p = .0563). From the meta‐analysis, the correlation coefficient between mRNA expression and protein expressions ranged from −.10 to .99, with a pooled effect of .76. In conclusion, we observed the moderate to strong associations between gene and protein expression for CB1 receptor in the human brain, which was validated by autoradiography. We combined the autoradiographic finding with PET of CB1 receptor, producing the density atlas map of CB1 receptor. From the meta‐analysis, the moderate to strong correlation was observed between mRNA expression and protein expressions across multiple genes. Further study is needed to investigate the relationship between multiple genes and in vivo proteins to improve and accelerate drug development.
Nutrition support teams: Institution, evolution, and innovation
Authors: Barrocas Albert , Schwartz Denise Baird , Bistrian Bruce R. , Guenter Peggi , Mueller Charles , Chernoff Ronni , Hasse Jeanette M. 
Published: November 2022

AbstractThe historical institution, evolution, and innovations of nutrition support teams (NSTs) over the past six decades are presented. Focused aspects of the transition to transdisciplinary and patient‐centered care, NST membership, leadership, and the future of NSTs are further discussed. NSTs were instituted to address the need for the safe implementation and management of parenteral nutrition, developed in the late 1960s, which requires the expertise of individuals working collaboratively in a multidisciplinary fashion. In 1976, the American Society for Parenteral and Enteral Nutrition (ASPEN) was established using the multidisciplinary model. In 1983, the United States established the inpatient prospective payment system with associated diagnosis‐related groupings, which altered the provision of nutrition support in hospitals with funded NSTs. The number of funded NSTs has waxed and waned since; yet hospitals and healthcare have adapted, as additional education and experience grew, primarily through ASPEN's efforts. Nutrition support was not administered in some instances by the “core of four” (physician, nurse, dietitian, pharmacist). The functions may be carried out by a member of the core of four not associated with the parent discipline, in accordance with licensure/privileging. This cross‐functioning has evolved into the adaptation of the concept of transdisciplinarity, emphasizing function over form, supported and enhanced by “top‐of‐license” practice. In some institutions, nutrition support has been incorporated into other healthcare teams. Future innovations will assist NSTs in providing the right nutrition support for the right patient in the right way at the right time, recognizing that nutrition care is a human right.