bluuebunny/crossref_metadata_2025_split huggingface dataset:
Corn hybrids for special purpose for livestock needs
Authors: Dzyubetskyi B. V. 
Published: October 2022

Carotenoids are one of the most common classes of plant pigments, which are used for the prevention of diseases in humans and animals and have wide prospects for use in clinical practice. The article describes the results of the selection of corn hybrids with an increased content of vitamins of the carotenoid complex. The content of carotenoids in the seeds of hybrids registered in Ukraine was determined, and the crtRB1 gene was identified, which is one of the key factors in the biosynthesis of carotenoids and is associated with their accumulation in the endosperm of corn. Three polymorphic regions of this gene were identified as carotenogenesis markers. According to the analysis, an allele favorable for the accumulation of β-carotene was found in DK253ЗСЗМ lines; DK 633/266zS, zM; DK 366zS, zM.
The technology for obtaining silage-type corn hybrids has been developed using inter-subspecies crossings and obtaining siliceous-sugar and tooth-like-sugar forms, which, with all other positive indicators, increases the fat content in the silage mass by 0.7 %. For the introduction of silage corn to the north, highly cold-resistant samples with a reduced seeding rate of loose corn with tooth-shaped and flinty corn were involved in hybridization.
The results of genetic and selection work in crop production create a powerful fodder base for the revival of animal husbandry and, on this basis, strengthen Ukraine's food security, which contributes to the health and longevity of the nation.
Assessment of nutritional approaches to nutritional requirements
Authors: Tsvigun A. Т. , Tsvigun O. А. 
Published: October 2022

The article presents the results of scientific, economic and physiological experiments conducted on Ukrainian black-spotted and red-spotted cows in different phases of lactation: Ukrainian (2009), English FIM (2004) and US NRC (2001) feeding standards. It was found that feeding dairy cows according to the Ukrainian (2009) English (FIM, 2004) and US NRC (2001) provides almost the same productivity.
In general, in all diets, the ratio between Calcium and Phosphorus was more than 2: 1, due to the excess in the diets of Calcium in two or more times. The ratio of Potassium to Magnesium in all diets was more than three, which is recommended for cows. It was found that the cation-anion balance in the diets of dairy cows was within physiological limits. Alkaline elements were predominant acidic, ie the balance was positive.
The concentration of crude fat in the NRC, 2001 and FIM, 2004 was significantly lower (26.3 - 53.0 %), and that the content of Calcium, Phosphorus, Sulfur, Iron, Cobalt, Manganese, Iodine and Selenium compared to Ukrainian standards in all phases of lactation. Cows that received rations according to NRC and FIM norms had higher milk productivity than Ukrainian ones, but unevenly during lactation phases.
Thus, in the first phase of lactation, according to English standards, milk yields were 3.3% higher, and according to US standards - 3.4% lower compared to Ukrainian standards. In the second phase of lactation milk yields according to Ukrainian and English standards were almost the same. Whereas under US standards, milk yields were 6.6% higher than in Ukraine.
In the third phase of lactation, milk yields were 3.9% higher in English standards and 8.1% higher in US standards than in Ukrainian standards.
ЕЛЕКТРОННІ ДОГОВОРИ ЯК ПРАВОВА ОСНОВА ЕЛЕКТРОННОЇ КОМЕРЦІЇ (ТОРГІВЛІ) В УКРАЇНІ
Authors: Мілаш В.С. 
Published: October 2022

У статті здійснено огляд сучасного стану та перспектив розвитку правового регулювання дого-вірних відносин в сфері електронної комерції, висвітлено та проаналізовано низку проблемних питань пов'язаних з формою та порядком укладення електронних договорів. За умов вітсутності законодавчого регламентації особливостей процедурно-технічних аспектів договірної співпрацї суб’єктів господа-рювання в електронному форматі, визначено потребу укладення генеральної угоди про загальні засади їх електронно-договірної взаємодії та режим доступу до електронних документів або включення до елек-тронного договору застереження про технічні аспекти договірної транзакції. Окрему увагу приділено електронним договорам в сфері зовнішньоекономічної діяльності, а також проблематиці смарт-контрактів. Актуалізовано необхідність модернізації та структурної кореляції актів господарського законодавства, спрямованих на регулювання договірних відносин в мережі Інтернет.
Ключові слова: електронна комерція, мережа Інтернет, електронна форма, електронні повідом-лення, електронний договір, мережевий договір, смарт-контракт.
Tratamiento de Drenajes Ácidos de Mina (DAM) mediante filtros elaborados con relave minero
Authors: Serrano-Uribe Brayan , Pardave-ivia Walter , Castillo-Martínez Camilo Hernando 
Published: October 2022

Los Drenajes Ácidos de Mina representan un riesgo para la salud humana y el medio ambiente cuando no se gestionan adecuadamente; con el fin de promover una estrategia innovadora y económica para su adecuado tratamiento enfocado en la neutralización del pH, se elaboraron cinco filtros con relave minero y otros materiales siguiendo la metodología de Desing Thinking. Los principales resultados muestran que es posible neutralizar los DAM con pH de 2,5 procedentes de una mina en California, Santander y dejar su efluente con valores de pH en un rango de 6,0 a 8,1. La conclusión es que la composición arcillosa con presencia de calcio y carbonatos en el relave minero permite que los filtros elaborados con este material puedan neutralizar los DAM a tal punto que es viable desarrollar un proyecto a gran escala en donde se incluyan estos filtros, lo cual permitirá tratar los DAM y mitigar los pasivos ambientales relacionados con la acumulación de residuos sólidos. Acid Mine Drainage represents a huge risk to people’s health and the environment when is not managed properly. Therefore, in order to promote an innovative and economic strategy that involves an adequate treatment focused on pH neutralization, five filters were made with mining tailings and other materials following the Design Thinking methodology. One of the main results show that it’s possible to neutralize AMD with a pH of 2.5, this, from a mine in the town of California, Santander, Colombia, leaving its effluent with pH values in a range of 6.0 to 8.1. The conclusion was that the clayey composition having the presence of calcium and carbonates, made with the mentioned material in the mining, allows filters to neutralize AMD to such an extent that it is feasible to develop a large-scale project where these are included, which in the future will allow treating AMD and mitigating environmental liabilities related to the accumulation of solid waste.
Neo-Marxist’s age-old experience: results and prospects of neo-Marxist’s capitalism theory
Authors: Mikhailouski Vadzim S. 
Published: October 2022

Neo-Marxist’s research program in social science exists in epistemological crisis condition. Excessive criticism, ideological engagement as well as final obscurity of capitalism are among the problems of neo-Marxism. As a result, there is no scientific answer on a key question of Marxism discussion why capitalism as a system of public mastery is still alive. Finish of crisis of neo-Marxist’s research program presumes presence of positivist’s finding in neo-Marxist’s research and applying of theory of complexity for efficient cognition of complicated system of capitalist’s reproduction. Complexity theory deconstruction of neo-Marxist’s concept of capitalism is realised in three main routes, such as theory of ideology, global order and revolution. There are three author’s conceptions overcoming current neo-Marxist’s problems are among results of present research. They are the conception of capitalist’s ideological minimalism, conception political support of global capitalism and the conception of ecological destruction of capitalism. Complexity theory deconstruction brings neo-Marxism back to scientific detection of objective factors of capitalist’s transformation within the framework of classical Marxist’s tradition but on the level of new theoretical and empiric determination.
A universal coating strategy for inhibiting the growth of bacteria on materials surfaces
Authors: Zhang Jie , Wang Min , Hu Liwei , Zhang Qiang , Chen Enni , Wang Zhongchao , Shi Yidong , Tan Lin , Xiao Shimeng 
Published: October 2022

The development of a versatile antibacterial coating, irrespective of material characteristics, is greatly attractive but still a challenge. In this work, mussel-inspired dopamine-modified sodium alginate (SA-DA) was successfully synthesized as the adhesion layer, and antibacterial coatings on three types of substrates, namely cotton fabric, aluminum sheet, and polyurethane membrane, were constructed through the layer-by-layer (LbL) deposition of polyhexamethylene guanidine and sodium alginate. Among the coated materials, the coated cotton fabric was systematically characterized, and the results showed that it still exhibited ideal hydrophilicity, and its liquid absorption capacity increased with an increase in the coating layers. The growth of Escherichia coli and Staphylococcus aureus was notably inhibited on the coated cotton fabric, and 10 coating bilayers achieved 100% inhibition of bacterial growth within 10 min. Furthermore, an ideal antibacterial ability maintained after 10 cycles of antibacterial trials or 50 washing or soaping cycles. In vitro evaluation of the hemostatic effect indicated that the coated cotton fabric could promote blood clotting by concentrating the components of blood and activating the platelets, and no significant hemolysis and cytotoxicity were observed in the coated cotton fabric. Moreover, the coated aluminum and polyurethane film also displayed an obvious antibacterial effect, which proved that the constructed coating could successfully adhere to the metal and polymer surfaces. Therefore, this work provided a proper way for the progress of a current antibacterial coating tactics for different substrate surfaces.
Predictive value of the presence of Prevotella and the ratio of Porphyromonas gingivalis to Prevotella in saliva for esophageal squamous cell carcinoma
Authors: Chen Xiaohui , Xian Bohong , Wei Junmin , Chen Yixiang , Yang Dongyang , Lai Xiaorong , Liu Lifang , Wu Yinghong , Lin Xiayi , Deng Yu , Zhang Huabin , Liu Wanwei , Qiao Guibin , Li Zijun 
Published: October 2022

BackgroundImbalance of oral salivary microbiota has been linked to the pathogenesis of a variety of systemic diseases, and oral bacterial species have been shown to be useful biomarkers for systemic diseases.This study aimed to characterize the alterations of oral microbiota in patients with esophageal squamous cell carcinoma (ESCC) and to evaluate the diagnostic performance of oral microbial biomarkers for ESCC.MethodsThe relative abundance of flora in saliva samples was analyzed by 16S rDNA sequencing, and differences in the species present in samples from ESCC patients and healthy controls (HCs) were identified by analyzing species diversity and performing LEfSe analysis. Receiver operating characteristic (ROC) curve analysis was applied to evaluate the diagnostic performance of the characteristic bacteria individually and in combination.ResultsDifferences in bacterial diversity indexes were observed for the saliva of ESCC patients versus HCs (P&lt;0.05), but principal coordinate analysis did not detect a significant difference in the composition of oral microbiota between ESCC patients and HCs (P&gt;0.05). LEfSe analysis showed that Leptotrichia, Porphyromonas (Pg), Streptococcus, Rothia, Lactobacillus and Peptostreptococcus were more abundant in ESCC patient saliva than in HC saliva, whereas Haemophilus, Alloprevotella (All), Prevotella_7, Prevotella (Pre), Prevotella_6, Pasteurellaceae and Pasteurellales were significantly less abundant in ESCC patient saliva (P&lt;0.05). From ROC curve analysis, Pg could detect ESCC with an area under the ROC curve (AUC) of 0.599, sensitivity of 62.2%, and specificity of 70%, whereas the ratio of Pg/Pre had an AUC of 0.791, sensitivity of 93.3%, and specificity of 62.3%. Moreover, the combination of the Pg/Pre and Pg/All ratios showed further improved diagnostic performance for ESCC (AUC=0.826) and even good sensitivity and specificity for the diagnosis of early ESCC (68.2% and 86%, respectively; AUC=0.786).ConclusionThis study shows that Pg in saliva can be used as a characteristic marker of ESCC, and the ratios of Pg/Pre and Pg/All offered significantly improved diagnostic performance, especially for early ESCC.
Association of non-alcoholic fatty liver disease with left ventricular changes in treatment-naive patients with uncomplicated hypertension
Authors: Catena Cristiana , Brosolo Gabriele , Da Porto Andrea , Donnini Debora , Bulfone Luca , Vacca Antonio , Soardo Giorgio , Sechi Leonardo A. 
Published: October 2022

Background and aimsCardiac structural and functional changes have been demonstrated in patients with non-alcoholic fatty liver disease (NAFLD). Because of the frequent association of NAFLD with hypertension, we aimed to examine the relationship of liver steatosis with left ventricular (LV) changes in patients with hypertension.Materials and methodsIn a cross-sectional study, we included 360 untreated, essential hypertensive patients who were free of major cardiovascular and renal complications. Liver steatosis was assessed by three different biochemical scores (NAFLD Liver Fat Score, LFS; Fatty Liver Index, FLI; Hepatic Steatosis Index, HSI). Echocardiography was performed with standard B-mode and tissue-Doppler imaging.ResultsLV hypertrophy was present in 19.4% and LV diastolic dysfunction in 49.2% of patients who had significantly higher body mass index (BMI), blood pressure (BP), and homeostatic model assessment (HOMA) index and higher frequency of the metabolic syndrome and liver steatosis that was defined by presence of 2 or more positive scores. LV mass index increased progressively across patients who had none, 1, or 2 or more liver steatosis scores, with associated progressive worsening of LV diastolic function. LV mass index was significantly and positively correlated with age, BMI, BP, HOMA-index, LFS, and HSI. Logistic regression analysis showed that age, BP, and liver steatosis scores independently predicted LV hypertrophy and diastolic dysfunction. Liver steatosis independently predicted LV dysfunction but not LV hypertrophy even after inclusion in analysis of the HOMA-index.ConclusionNAFLD is associated with LV hypertrophy and diastolic dysfunction in untreated patients with hypertension. In hypertension, NAFLD could contribute to LV diastolic dysfunction with mechanisms unrelated to insulin resistance.
Antithrombotic therapy in peripheral arterial disease
Authors: Espinola-Klein Christine , Weißer Gerhard , Schmitt Volker , Schwaderlapp Melanie , Munzel Thomas 
Published: October 2022

BackgroundPatients with peripheral arterial disease (PAD) are at increased risk for major adverse cardiovascular events (MACE) such as cardiovascular death, myocardial infarction, and stroke as well as major adverse limb events (MALE) such as amputation and acute limb ischemia. Therefore, prevention of thrombotic events is crucial to improve the prognosis of PAD patients. This review article concludes current evidence and guideline recommendations about antithrombotic therapy in PAD patients.Antithrombotic therapy is highly effective to reduce MACE and MALE events in PAD patients. Recently, the concept of dual pathway inhibition (low-dose rivaroxaban plus acetylic salicylic acid (ASA) has been tested in the COMPASS and VOYAGER-PAD trial. Compared to ASA alone dual pathway inhibition was superior to prevent MACE and MALE. After peripheral revascularization, in particular the risk for acute limb ischemia was reduced. In contrast, the risk for major bleeding is increased. Therefore, current guidelines recommend the combination of low-dose rivaroxaban and ASA in PAD patients with low bleeding risk. In patients with high bleeding risk, a single antiplatelet drug (preferable clopidogrel) is indicated. In patients with atherosclerotic vascular disease and indication for oral anticoagulation, no additional antiplatelet drug is necessary, as this would increase the risk of bleeding without improving the prognosis.ConclusionAntithrombotic treatment reduces MACE and MALE and is recommended in all patients with PAD. Individual bleeding risk should always be considered based on the current data situation and an individual benefit-risk assessment must be carried out.
Independent association of PCSK9 with platelet reactivity in subjects without statin or antiplatelet agents
Authors: Wang Shuai , Fu Di , Liu Huixing , Peng Daoquan 
Published: October 2022

Background and aimsProprotein convertase subtilisin/kexin type 9 (PCSK9) levels could predict cardiovascular event in patients with well-controlled LDL-C levels, suggesting an LDL-independent mechanism of PCSK9 on the cardiovascular system. Accumulating evidence suggests PCSK9 might be associated with increased platelet reactivity. This study aimed to assess the relationship between PCSK9 levels and platelet reactivity in subjects not taking statins or antiplatelet agents.MethodsA cross-sectional study was conducted to investigate the independent contribution of PCSK9 to platelet activity by controlling for the potential confounding factors. The study population included 89 subjects from a health examination centre who underwent routine annual health check-ups or had an examination before a selective operation. Subjects taking statins or antiplatelet agents were excluded. Adenosine diphosphate (ADP)-induced platelet aggregation was determined by PL-11 platelet analyzer using impedance aggregometry and plasma PCSK9 levels were determined using an ELISA. Serum Lipid profile was assessed by measuring the concentration of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG), with low-density lipoprotein cholesterol (LDL-C) being directly measured using enzymatic techniques. The association between PCSK9 and platelet reactivity was investigated.ResultsThe study subjects were composed of 53 males and 36 females with an average age of 55 (±11) years old. The univariate correlation analysis showed significant correlation between ADP-induced maximal aggregation rate (MAR) and PCSK9 (r = 0.55, p &lt; 0.001) as well as TC (r = 0.23, p = 0.028), LDL-C (r = 0.27, p &lt; 0.001), and PLT (r = 0.31, p = 0.005). Being male (41.2% vs. 46.6, p = 0.04) and smoking (37.4 vs. 46.2%, p = 0.016) were associated with lower ADP-induced MAR than being female and non-smoking. However, there is no correlation between PCSK9 and AA-induced platelet maximal aggregation rate (r = 0.17, p = 0.12). Multiple regression analysis suggested that PCSK9 contributed independently to ADP-induced maximal aggregation rate (β = 0.08, p = 0.004) after controlling for the effect of TC, LDL-C, PLT, being male, and smoking.ConclusionsPCSK9 is positively associated with platelet reactivity, which may partly account for the beneficial effect of PCSK9 inhibition in reducing the risk of major adverse cardiovascular events after acute coronary syndrome (ACS).
Preoperative red cell distribution width is associated with postoperative lymphovascular invasion in prostate cancer patients treated with radical prostatectomy: A retrospective study
Authors: Wang Fangming , Liang Jing , Yang Feiya , Liu Fei , Han Sujun , Xing Nianzeng 
Published: October 2022

PurposeTo investigate the relationship between baseline clinicopathological and laboratory variables especially hematological parameters and lymphovascular invasion (LVI) in patients who underwent radical prostatectomy (RP).MethodsWe retrospectively evaluated 348 prostate cancer (PCa) patients who underwent RP in our center between May 2018 and June 2021. We divided them into non-LVI and LVI groups based on LVI status, and compared clinicopathological characteristics between non-LVI and LVI groups. Clinicopathological parameters including age, body mass index (BMI), history of hypertension and diabetes mellitus, neoadjuvant hormonal therapy (NHT), pathological stage T (pT) and lymph node status (pN), ISUP (international society of urological pathology) grade, positive surgical margin (PSM) rate, and hematological parameters containing prostate-specific antigen (PSA), whole blood parameters and inflammatory indexes were collected. The association between the clinicopathological parameters and the presence of LVI was identified by multivariate logistic regression analysis.ResultsThe pathological results of the RP specimen consisted of 53 (15.2%) patients with LVI and 295 (84.8%) cases without LVI. The level of PSA, percentages of advanced pT and grade, pN1, and PSM were significantly higher in the LVI group when compared with the non-LVI counterpart (p&lt;0.001, p&lt;0.001, p&lt;0.001, p&lt;0.001, p=0.007, respectively). Among the whole blood parameters, only red cell distribution width (RDW) was significantly different (41.2 ± 2.5 vs. 42.1 ± 3.1, p=0.035). Multivariate regression analysis demonstrated that RDW and NHT were negatively correlated with the presence of LVI (OR = 0.870, p=0.024; OR = 0.410, p=0.025), while PSA, ISUP, and pT were positively correlated with the presence of LVI (OR=1.013, p=0.005; OR =1.589, p=0.001; OR=1.655, p=0.008) after adjusting for confounding factors.ConclusionsRDW rather than other whole blood parameters was independently and negatively associated with the presence of LVI in PCa patients, suggesting that RDW might play an essential role in PCa invasion.
Sources, spectral characteristics, and fluxes of dissolved organic matter in coastal groundwater and river water in western Guangdong, China
Authors: Wang Chao , Hu Xuanyu , Liu Yilin , Jin Guangzhe 
Published: October 2022

Groundwater discharge and river runoff are two important ways for allochthonous dissolved organic matter (DOM) to enter the ocean, but they vary spatially. Currently, the source, composition, and efflux of groundwater and riverine dissolved organic matter to the tropical northern South China Sea remain poorly understood, preventing an accurate estimate of coastal carbon budgets. In July 2021, nine groundwater and six river water samples were collected along the coast of western Guangdong and were characterized using dissolved organic carbon (DOC) analysis, UV-visible, and fluorescence spectroscopy techniques. Atypical absorption spectra were strongly observed in groundwaters, which were mainly attributed to the high-level nitrates. An exponential-Gaussian equation showed that the average contribution of nitrate to total groundwater absorbances was up to 36% at ∼ 300 nm. Groundwater showed lower levels of DOC, colored dissolved organic matter (CDOM), and fluorescent dissolved organic matter (FDOM) as compared to river water, whereas the humic-like components dominated both groundwater and river water FDOM pools. Higher values of spectral slope S350–400, fluorescence index, biological index, and the ratio of two humic-like fluorescence jointly reveal that groundwater DOM mainly originated from microbial activities, whereas the river water DOM had considerable contribution from terrestrial soil. High precipitation, natural or agricultural land types, and a lack of seawater intrusion are expected to cause low levels of groundwater DOM in western Guangdong. Among 52 rivers from across the globe, high watershed productivity and precipitation may lead to high–moderate DOM levels in river water in western Guangdong. The DOM fluxes via fresh groundwater discharge in western Guangdong are comparable in magnitude to those by river runoff, accounting for 16%–18% of the Pearl River DOM fluxes. Based on the slopes of linear correlations between CDOM and DOC obtained in groundwater samples and 52 rivers, as well as the data on water fluxes and DOC levels, the global CDOM fluxes via groundwater discharge and river runoff are estimated to be 3.5–12.2 × 1012 m2 yr−1 and 4.3 ± 0.3 × 1014 m2 yr−1, respectively, highlighting the importance of considering groundwater discharge and river runoff in coastal carbon budgets.