bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inheritance of Resistance to Race 5 of Powdery Mildew fungus Podosphaera xanthii in Melon and development of Race 5-Specific High Resolution Melting Markers
Authors: Hong Jeong-Eui , Hossain Mohammad Rashed , Jung Hee-Jeong , Nou Ill-Sup 
Published: March 2022

Abstract
Powdery mildew (PM), caused by the biotrophic fungus Podosphaera xanthii, drastically reduces the yield and quality of melon (Cucumis melo L.). Knowledge on the genetic control and high throughput molecular markers linked with resistance against this disease are essential for breeding programs. The bioassay study of the F1 and F2 populations derived from the parents, ‘PMR 5’ (♂) and ‘SCNU1154’ (♀) revealed a monogenic dominant nature of resistance to the devastating race, race 5. Besides, we developed three SNP based high resolution melting markers, PMm-HRM-1, PMm-HRM-2, and PMm-HRM-3 based on the previously identified SNPs on chromosome 12 and validated those using 10 melon lines and 137 F2 population. Among these, the SNP of marker PMm-HRM-1 causes a missense mutation (Lysine → Glutamic acid) in the LRR region of MELO3C002393 and were able to distinguish the resistant vs susceptible genotypes from 10 diverse melon population and the segregating F2 population with more than 90% genotyping efficiency. Other two markers were based on intergenic SNPs and had more than 80% genotyping efficiency F2 population. These markers will be helpful to melon breeders to develop melon cultivars resistant to P. xanthii race 5 via marker assisted breeding programs.
Evolutionary analyses of Georgian Rabies Lyssavirus strains and their genetically most closely related conspecifics from elsewhere provide evidence suggesting risks for dog-to-cattle rabies transmission as well as this disease coinfections and transborder transmission
Authors: Tabatadze Leila , Gabashvili Ekaterine , Kobakhidze Saba , Lomidze George , Loladze Jimsher , Tsitskishvili Levan , Kotetishvili Mamuka 
Published: March 2022

Abstract
Genetic relationships between Rabies Virus (RABV) isolates recovered from dog, jackal, and cattle in Georgia, and their genetically most closely related conspecifics from elsewhere, were determined, using the DNA analysis of nucleoprotein (N) gene loci. Multiple isolates from dogs and cattle were found to share the same sequence types (STs), being suggestive of the risks of dog-to-cattle rabies transmission in Georgia. Exhibiting population selective sweeps and expansion, as well as genetic recombination, the evolutionary analyses of Georgian RABV strains and their conspecifics from Russia, Turkey, Iran, and elsewhere, provided further evidence for rabies coinfections and its transborder transmission.
Exploring pain interference and self-perceived health status in children with Osteogenesis Imperfecta - A cross-sectional study
Authors: Provenzano Anna Hallin , Åström Eva , Löwing Kristina 
Published: March 2022

Abstract
Purpose
To explore presence of chronic pain, pain interference in daily life, and self-perceived health status in children with Osteogenesis Imperfecta (OI).
Methods
Children with OI, aged 6–18 years, were recruited consecutively to this cross-sectional study. Participants answered a standardised interview including five pre-structured questions, Numerical Rating Scale (NRS), the Pain Interference Index, and the PROMIS-25.
Results
Twenty-eight children (median: 11 years, IQR 6) with OI type I, III, or IV participated. Pain was present in 27 of 28 children, and interfered in their everyday life regardless of OI-type, sex, and age. The median NRS for average pain intensity was 4 (IQR 2), the median for pain frequency was 2–3 times/week, and the median frequency of school absence due to pain was 2–3 times per month. Pain in the feet was more frequently reported in children with type I (p = 0.032), and pain in the hip was more often reported in children ≥ 13 years (p = 0.011). Self-perceived health status for mobility was lower than the general population, and lowest for children with type III (p = 0.016). Pain interference was associated with children’s self-perceived health status (rs=0.84, p < 0.001).
Conclusion
Almost all children experienced pain, which interfered in children’s everyday lives, affected participation in various activities and was associated with reduced self-perceived health status. If children avoid physical activities because of pain, it might cause a vicious circle of inactivity, which further decreases bone density and increase the risk of fractures. The results emphasize the importance to offer adequate pain reducing interventions.
Poor disease knowledge is associated with higher healthcare service use and costs among patients with cirrhosis: an exploratory study
Authors: Valery Patricia , Bernardes Christina , Hayward Kelly , Hartel Gunter , Haynes Katelin , Gordon Louisa , Stuart Katherine , Wright Penny , Johnson Amy , Powell Elizabeth 
Published: March 2022

Abstract
Background: Optimal management of cirrhosis is complex, and patients often lack knowledge and skills, which can affect self-management. We assessed patient knowledge about cirrhosis and examined whether knowledge was associated with clinical outcomes, healthcare service use, and healthcare costs. A cross-sectional ‘knowledge survey’ was conducted during 2018-2We assessed patient knowledge about cirrhosis and explore whether knowledge was associated with clinical outcomes, healthcare service use, and costs. METHODS: Patients with cirrhosis (n=123) completed a ‘knowledge survey’. We calculated the proportion of correct answers to eight questions deemed to be “key knowledge” about cirrhosis by an expert panel, and dichotomized patients as ‘good knowledge’/‘poor knowledge’. Clinical data, healthcare costs, and health-related quality of life (SF-36) were available. RESULTS: 58.5% of patients had ‘good knowledge’ about cirrhosis. Higher education level was associated with higher odds of having ‘good knowledge’ about cirrhosis (adjusted-OR=5.55, 95%CI 2.40-12.84). Compared to patients with ‘poor knowledge’, those with ‘good knowledge’ had a higher health status in the SF-36 physical functioning domain (p=0.011), fewer cirrhosis-related admissions (adjusted incidence rate ratio [IRR]=0.59, 95%CI 0.35-0.99) and emergency presentations (adj-IRR=0.34, 95%CI 0.16-0.72), and more planned one-day cirrhosis admissions (adj-IRR=3.96, 95%CI 1.46-10.74). The total cost of cirrhosis admissions was lower for patients with ‘good knowledge’ (adj-IRR=0.30, 95%CI 0.29-0.30).Conclusion: Poor disease knowledge is associated with increased use and total cost of healthcare services. Targeted educational interventions to improve patient knowledge may be an effective strategy to promote a more cost-effective use of healthcare services.
Pharmacophore Generation, 3D-QSAR study, and Molecular Docking of pyrazoline derivatives for antiamoebic activity against HM1: IMSS strain of E. histolytica.
Authors: Bouacha Samir 
Published: March 2022

Abstract
Activity against HM1: IMSS strain of E. histolytica has been carried out through the PHASE program (Schrodinger-USA). The best pharmacophore model generated consisted of five features DHHHR-4: three Hydrophobic (H), one aromatic ring (R), and one H-bond donor (D). Field-Based 3D-QSAR model studies were applied on a series (60 compounds) of pyrazoline derivatives. The best prediction was obtained with a gaussian field combined steric, electrostatic, hydrophobic, hydrogen bond donor, and acceptor fields (r2=0.837, q2= 0.766). The contour maps resulting from the best field-based QSAR model were exploited to discover the structural properties related to the biological activity of this series of analogous molecules. Structure-based docking studies were performed to elucidate the intermolecular interaction between pyrazoline derivatives and the 5ZFE receptor. Active ligands 35 and 36 have the highest activity and the best docking scores. The insilico ADMET screening of these active ligands was also performed and the values of all the properties are within the recommended values. The information obtained from pharmacophore, 3D QSAR model, molecular docking, and ADMET screening can be used to discover pyrazoline derivatives that increase antiamoebic activity.
Multi-Objective Entropy FCSVD: Contextual Spectrum Analysis for Prototyping Recommender Systems
Authors: Mirabedini Seyed Javad , Rodpysh Keyvan Vahidy , Banirostam Touraj 
Published: March 2022

Abstract
In recent years explosive increase and variety of information due to the progress of the internet. Therefore, decision making in different fields has faced different challenges. Recommender systems provide personalized services to users by identifying users' interests, filtering information, and managing information, especially contextual information. Similarity criteria based on contextual information is a way to reduce the challenge of starting a cold. We argue that similarity is a vague concept, and we more realistic results in recommender systems using fuzzy logic. Applying fuzzy logic to contextual information can be an effective way to identify ambiguities and uncertainties in measuring the similarity of items and users. In this paper, we present a new multilevel contextual fuzzy similarity criterion with SVD matrix for recommending systems called FCSVD Which is based on a combination of similarity criteria PSS, fuzzy rules and contextual information. The results show an improvement in the accuracy of the method recommendations FVSVD compared to the CACF, CTLSVD, MF-LOD and CFSVD methods according to evaluation measure Precision, Recall, F1-score.
Usage of campaign to encourage travel and personal infection prevention behaviors in Japan: a cross-sectional study
Authors: Shimoda Hayato , Nagata Tomohisa , Ishimaru Tomohiro , Hino Ayako , Ando Hajime , Muramatsu Keiji , Tateishi Seiichiro , Tsuji Mayumi , Mori Koji 
Published: March 2022

Abstract
Background: The Go To Travel campaign in Japan was launched to subsidize travel and accommodation costs for tourists through vouchers that could be used at domestic destinations. We examined the relationship between using Go To Travel and infection prevention behaviors.Methods: We conducted a cross-sectional study of 26,637 workers who responded to a large-scale questionnaire survey about COVID-19 under the pandemic in Japan. We undertook logistic regression analysis.Results: Among the 26,637 participants, 7,959 (30%) used Go To Travel. Compared with non-Go To Travel users, we observed statistically significant differences in multivariate analysis with Go To travel users for the following: gargling (odds ratio [OR], 0.91; 95% confidence interval [CI], 0.87–0.97; P = .001); room ventilation (OR, 0.94; 95% CI, 0.88–0.99; P = .017); and washing hands (OR, 0.94; 95% CI, 0.89–1.00; P = .036).Conclusions: We observed the tendency for Go To Travel users not to implement some infection prevention behaviors. It is necessary to continue the encouragement of applying infection prevention behaviors: implementing such travel support policies as Go To Travel during COVID-19 could spread the risk of infection.Trial registration: Not applicable
Association of age, sex, nutritional risk, serum 25-hydroxyvitamin D, serum uric acid, and exercise capacity with bone mineral density in elderly and very elderly inpatients: a cross-sectional study.
Authors: Li Han , Han Dongmei , Gong Weiwei , Mao  , Gan  , Shu  , Zhou  , Xu Lei , Hou  , Cai  , Zhou Mingcheng , Lai Xueli 
Published: March 2022

Abstract
Objective: Risk factors for osteoporosis may vary according to different populations. Much remains unknown in elderly, especially very elder patients. We aim to investigate the association of age, sex, nutritional risk, serum 25-hydroxyvitamin D, serum uric acid, and physical function with bone mineral density in elderly and very elderly patients. Methods: Elderly hospitalized patients at the First Rehabilitation Hospital of Shanghai, China, from December 2019 to December 2020 who were older than 60 years old were selected. Combined with an activities of daily living (ADL) scale and nutritional assessment, the causes of BMD reductions in elderly and elderly patients were analysed. Results: A total of 94 patients (aged 63-98 years at baseline, with an average age of 83.56 ± 8.37 years) were enrolled. With increasing age, the BMD of the lumbar spine, femoral neck, and femoral shaft of elderly patients significantly decreased, and the incidence of osteoporosis (OP) significantly increased. The BMD of the lumbar spine was negatively correlated with female sex and positively correlated with serum 25-hydroxyvitamin D levels, the difference between actual body weight and ideal body weight, and blood uric acid levels; the BMD of the femoral neck was negatively correlated with age and female sex and positively correlated with height and geriatric nutrition risk index (GNRI) score; the BMD of the femoral shaft was negatively correlated with female sex and positively correlated with exercise capacity (ADL score). Conclusion: With increasing age, the BMD of the lumbar spine and the femoral shaft significantly decreased, and the incidence of OP significantly increased in elderly and very elderly patients. Aric acid may protect bone health in elder patients. Early attention to the nutritional status, exercise capacity, 25-hydroxyvitamin D level, and blood uric acid level in the elderly population can help identify high-risk elderly patients with OP.
Adaptive Individualized high-Dose preoperAtive (AIDA) chemoradiation in high-risk rectal cancer: a phase II trial
Authors: GUIDO Alessandra , CUICCHI Dajana , CASTELLUCCI Paolo , CELLINI Francesco , FABIO Francesca DI , LLIMPE Fabiola Lorena ROJAS , STRIGARI Lidia , BUWENGE Milly , CILLA Savino , DEODATO Francesco , MACCHIA Gabriella , GALIETTA Erika , GOLFIERI Rita , ARDIZZONI Andrea , ZAGARI Rocco Maurizio , FANTI Stefano , POGGIOLI Gilberto , FUCCIO Lorenzo , MORGANTI Alessio Giuseppe 
Published: March 2022

Abstract
Purpose
To evaluate the pathological complete response (pCR) rate of locally advanced rectal cancer (LARC) after adaptive high-dose neoadjuvant chemoradiation (CRT) based on 18 F-fluorodeoxyglucose positron emission tomography/computed tomography (18 F-FDG-PET/CT).
Methods
The primary endpoint was the pCR rate. Secondary endpoints were the predictive value of 18 F-FDG-PET/CT on pathological response and acute and late toxicity. All patients performed 18 F-FDG-PET/CT at baseline (PET0) and after 2 weeks during CRT (PET1). The metabolic PET parameters were calculated both at the PET0 and PET1. The total CRT dose was 45 Gy to the pelvic lymph nodes and 50 Gy to the primary tumor, corresponding mesorectum, and to metastatic lymph nodes. Furthermore a sequential boost was delivered to a biological target volume defined by PET1 with an additional dose of 5 Gy in 2 fractions. Capecitabine (825 mg/m2 twice daily orally) was prescribed for the entire treatment duration.
Results
Eighteen patients (13 Males, 5 Females; median age 55 years [range, 41–77 years]) were enrolled in the trial. Patients underwent surgical resection at 8–9 weeks after the end of neoadjuvant CRT. No patient showed grade > 1 acute radiation-induced toxicity. Seven patients (38.8%) had TRG = 0 (complete regression), 5 (27.0%) showed TRG = 2, and 6 (33.0%) had TRG = 3. Based on the TRG results, patients were classified in two groups: TRG = 0 (pCR) and TRG = 1,2,3 (non pCR). Accepting p < 0.05 as the level of significance, at the Kruskal-Wallis test, the medians of baseline-MTV, interim-SUVmax, interim-SUVmean, interim-MTV, interim-TLG, and the MTV reduction were significantly different between the two groups. 18 F-FDG-PET/CT was able to predict the pCR in 77.8% of cases.
Conclusions
Our results showed that a dose escalation on a reduced target in the final phase of CRT is well tolerated and able to provide a high pCR rate.
The efficacy and safety of immunotherapy and palliative radiotherapy in metastatic non-small cell lung cancer patients: a systematic review and meta-analysis
Authors: Cao Dedong , Zhou Dingjie , Zhao Nan , Xu Huilin , Zheng Yongfa , Ming Pingpo , Xu Ximing , Jiang Zhenmin , Ge Wei 
Published: March 2022

Abstract
Objective:Palliative radiotherapy (pRT) is usually used in the treatment of metastatic non-small cell lung cancer (mNSCLC). However, whether pRT could influence the outcomes of mNSCLC patients who considered immune checkpoint inhibitors (ICIs) as maintenance treatment is still under debate. Here we did a systematic review and meta-analysis to assess the efficacy and safety of this combination strategy in mNSCLC patients.Methods:The PubMed, Cochrane and Embase databases were searched for prospective studies and randomized controlled trials (RCTs). The main outcomes were overall survival (OS), progression-free survival (PFS), disease control rate (DCR), objective response rate (ORR), and treatment-related adverse events. All data was analyzed with Stata v 16.0 and Review Manager version 5.4 software.Results:A total of 12 studies were included. pRT plus ICIs was significantly associated with improved DCR (OR=2.27, 95%CI [1.27, 4.04]; p=0.008), OS (HR=0.66, CI [0.54, 0.80]; p<0.0001) and PFS (HR=0.60, CI [0.44, 0.81]; p=0.0009). In safety analyses of III-IV grade adverse events (AEs), patients receiving ICIs plus pRT showed no statistically significant difference (p=0.43) when compared with those in the control group.Conclusion:Patients with mNSCLC may benefit from the ICI and pRT combination therapy with acceptable incidence of grade III-IV AEs.
Quantifying heterologous gene expression during ectopic MazF production in Escherichia coli
Authors: Nikolic Nela , Sauert Martina , Albanese Tanino G. , Moll Isabella 
Published: March 2022

Abstract
ObjectiveMazF is a sequence-specific endoribonuclease-toxin of the MazEF toxin-antitoxin system, which cleaves single-stranded RNA regions at ACA sequences in Escherichia coli. The MazEF system has been used in various biotechnology and synthetic biology applications. In this study, we infer how ectopic mazF overexpression affects production of heterologous proteins. To this end, we quantified the levels of fluorescent proteins expressed in E. coli from reporters translated from the ACA-containing or ACA-less mRNAs. Additionally, we addressed the impact of the 5’-untranslated region of these reporter mRNAs under the same conditions by comparing expression from mRNAs that comprise (canonical mRNA) or lack this region (leaderless mRNA).ResultsFlow cytometry analysis indicates that during mazF overexpression, fluorescent proteins are translated from the canonical mRNAs, as well as from the leaderless mRNAs. Our analysis further indicates that longer mazF overexpression generally increases the concentration of fluorescent proteins translated from ACA-less mRNAs, however it also substantially increases bacterial population heterogeneity. Finally, our results suggest that the strength and duration of mazF overexpression should be optimized for each experimental setup, to maximize the heterologous protein production and minimize the amount of phenotypic heterogeneity in bacterial populations, which is unfavorable in biotechnological processes.
CNC Synthesis and Fabrication of PVA/TiO2/CNC bio-nanocomposite thin film for biomedical applications
Authors: Raj P L Joseph , Kulkarni Seema A , Krishnan Kalimuthu , Selvam S Periyar 
Published: March 2022

Abstract
Polyvinyl alcohol (PVA) based films with cellulose nanocrystals (CNC) and Titanium dioxide (TiO2) in varying concentrations have been prepared and characterised in this study, to discover their application in the biomedical field. Acid hydrolysis method is applied to extract CNC from pea peel waste fibres (PPWF) and their sizes were determined. The films - PVA/CNC/T0.5, PVA/CNC/T1.0 and PVA/CNC/T1.5 were subjected to biophysical, microscopic and mechanical property characterization. Through SEM and FTIR analysis, uniform distribution and successful incorporation of CNC and TiO2 has been confirmed. From the X-ray diffraction pattern of the films, it could be noted that the crystallinity increased with the addition of CNC and rutile TiO2. The tensile strength of the films also greatly improved with the CNC reinforcement and increase in TiO2 content in the films. Overall, the results of this study suggest that the PVA/CNC/ TiO2 films have the potential to find application in biomedical devices.
Detection of pyrophosphate and alkaline phosphatase activity based on PolyT single stranded DNA - copper nanoclusters
Authors: Yao Liu , Li Xin , Li Hong , Liao Zhibin , Ning Ge , Wu Yaohui , Wang Yonghong 
Published: March 2022

Abstract
The determination of pyrophosphate and alkaline phosphatase activity plays a significant role in medical diagnosis. In this work, a label-free "ON-OFF-ON" fluorescence strategy is developed for the analysis of pyrophosphate and alkaline phosphatase activity. Using PolyT single strand DNA as templates to synthesize fluorescent copper nanoparticles, the coordination effect of pyrophosphoric acid on Cu2+ inhibited the generation of fluorescence. Afterwards, the addition of alkaline phosphatase into hydrolyze pyrophosphoric acid resulted in the release of Cu2+, whereby the fluorescence intensity could be recovered. Thereupon enhanced-sensitivity for alkaline phosphatase was obtained (0.1 mU/L), much better than previously reported methods. Meanwhile, it could be performed directly in homogeneous solution, which was very close to the actual activity level of alkaline phosphatase under physiological conditions. Likewise, satisfactory results were also obtained in specificity assessment, which demonstrated its potential application in clinical diagnosis. Notably, a new, sensitive, low-cost, short-time, and high-sensitivity platform for alkaline phosphatase detection was constructed, and the design of biosensor using DNA-templated Copper nanoclusters (CuNCs) was instructed in this study.
Variation of antinutrients in faba bean cultivars during seed development
Authors: Nurmi Markus , Kahala Minna , Pihlava Juha-Matti , Hellström Jarkko , Bragge Rina , Pihlanto Anne 
Published: March 2022

Abstract
Several chemical compounds of seven faba bean cultivars—Sampo, Louhi, Talia, Tiffany, Julia, Gloria, and Vroma—grown in Finland were determined. The effect of seed maturity on the levels of vicine/convicine (vc), trypsin inhibitors, condensed tannins, phytic acid, lectins, and oligosaccharides was analyzed. The overall vc ratio remained similar in all the samples, and the largest amounts of vc were found in the early stage of the bean and decreased during maturation. Talia and Gloria had lower trypsin inhibition than the other cultivars. The smallest amounts of condensed tannins were found in the Talia and Gloria cultivars, and the largest in the Tiffany and Julia cultivars. Overall, it seems that maturation decreased the amount of condensed tannins. The fully mature faba bean contained an average of 10.3 to 13.4 g/kg (DW) phytic acid, and there was no difference between the seven cultivars. The highest lectin activity was found in the Sampo, Julia, and Tiffany cultivars, and the lowest activity was in Vroma and the mature seeds of Talia and Louhi. The simple sugars (rhamnose, galactose, glucose, sucrose, and fructose) were dominant in the early developmental stage, whereas the amount of long chain oligosaccharides (stachyose and verbascose) increased during maturation of beans, and the level of raffinose was similar in all developmental stages. In conclusion, the content of chemical compounds depended on the maturation stage and the faba bean cultivar. The low content of stachyose, verbascose, and raffinose in young Tiffany and Julia beans is beneficial when consumed as green bean.