bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactions of Colorectal Cancer, Dietary Fats, and Polymorphisms of Arachidonate Lipoxygenase and Cyclooxygenase Genes: A Literature Review
Authors: Gholamalizadeh Maryam , Majidi Nazanin , Tajaddod Shirin , Abdollahi Sepideh , Poorhosseini Seyed Mohammad , Ahmadzadeh Mina , Naimi Joubani Mohammad , Mirzaei Dahka Samaneh , Shafaei Hanieh , Hajiesmaeil Mogge , Alizadeh Atiyeh , Doaei Saeid , Houshiar-Rad Anahita 
Published: July 2022

ObjectiveGenetics and dietary factors play important roles in the development of colorectal cancer (CRC). However, the underlying mechanisms of the interactions between CRC, gene polymorphisms, and dietary fat are unclear. This review study investigated the effects of polymorphisms of arachidonate lipoxygenase (ALOX) and cyclooxygenase (COX) genes in the association between CRC and dietary fat.MethodsAll the related papers published from 2000 to 2022 were collected from different databases such as PubMed, Science Direct, Scopus, and Cochran using related keywords such as colorectal cancer, ALOX, COX, polymorphism, and dietary fat. Non-English and unrelated documents were excluded.ResultsSome single-nucleotide polymorphisms (SNPs) in the ALOX and COX genes, such as rs2228065, rs6413416, and rs4986832 in the ALOX gene, and rs689465 in the COX gene may play significant roles in the association between the risk of CRC and dietary fats. SNPs of ALOX and COX genes may influence the effects of dietary fatty acids on the risk of CRC.ConclusionSome polymorphisms of the ALOX and COX genes may have important roles in the effects of dietary fat on the risk of CRC. If future studies confirm these results, dietary recommendations for preventing colorectal cancer may be personalized based on the genotype of the ALOX and COX genes.
The Efficacy and Safety of Diyushengbai Tablet on Preventing and Treating Leukopenia Caused by Radiotherapy and Chemotherapy Against Tumor: A Systematic Review and Meta-Analysis
Authors: Xu Xiaoqing , Li Hongmin , Hu Xiwen , Liu Juan , Zhao Fang , Xie Kaiyong , Wang Jun 
Published: July 2022

Background: Leukopenia is one of the side effects of radiotherapy and chemotherapy. Diyushengbai tablet (DYT) is used to prevent and treat leukopenia caused by various reasons. A meta-analysis was performed to systematically analyze the therapeutic effects of DYT on preventing and treating leukopenia caused by radiotherapy and chemotherapy.Objectives: This study aimed to systematically evaluate the efficacy and safety of DYT in preventing and treating leukopenia caused by radiotherapy and chemotherapy.Methods: We performed a comprehensive literature search of electronic databases such as PubMed, The Cochrane Library, China Knowledge Network (CNKI), China Biomedical Literature Database (CBM), Wanfang Data Knowledge Service Platform, and VIP, through November of 2021. The scanning reports deadline is until November 2021. The bias risk evaluation criteria developed by the Cochrane collaborative organization were used to evaluate the literature quality of the included studies. The RevMan5.4 software was used to analyze the data, and the Stata16.0 was used to perform the Egger test.Results: After selecting all the databases, a total of 41 reports which involved 3,793 cases were analyzed. Meta-analysis showed that DYT could significantly reduce the white blood cell (WBC) suppression caused by radiotherapy and chemotherapy and improve the patients’ WBC counts and neutrophils, compared with the efficacy of other oral WBC-elevating drugs such as Leucogen tablets and Batilol tablets and additional utilization of granulocyte colony-stimulating factor (G-CSF). The results of meta-analysis showed that for preventive medication purpose, the overall incidence of leukocyte suppression was [RR = 0.74, 95%CI (0.59, 0.92), p = 0.006], and the white blood cell count was [MD = 1.12, 95%CI (0.95, 1.29), p &lt; 0.00001]; while for therapeutic purpose, the incidence of overall leukocyte suppression was [RR = 0.61, 95%CI (0.38, 0.95), p = 0.03], and the white blood cell count was [MD = 1.20, 95%CI (0.77, 1.62), p &lt; 0.00001]. More importantly, the additional use of DYT can reduce the application amount of G-CSF. The results showed that the application of G-CSF can be reduced by an average of 1.57 from the beginning of treatment to return normal white blood cells around 2.23 in two cycles of chemotherapy.Conclusion: DYT is more effective in preventing and treating leukopenia caused by radiotherapy and chemotherapy than other oral WBC-elevating drugs, which have a high clinical value.
Irrational Use of Selected Herbal Medicines During Pregnancy: A Pharmacoepidemiological Evidence From Yemen
Authors: Ahmed Mansoor , Hwang Jung Hye , Ali Mohammed Nasr , Al-Ahnoumy Shafekah , Han Dongwoon 
Published: July 2022

Background: Recent research indicates irrational use of herbal medicine (HM) during pregnancy that can be harmful to the mother and development of baby. However, no study has been conducted to explore the use of HM among pregnant women in a conflict region.Methods: This was a cross-sectional research conducted in July and August in the year 2017, at three hospitals in Sana’a, Yemen. Postpartum women were interviewed via the structured instrument to collect data on patterns of HM used during pregnancy.Results: A total of three hundred postpartum women participated in the study, with 59.3% (178) of them using at least one modality of HM in the previous pregnancy. Cinnamon (Cinnamomum verum L), ginger (Zingiber officinale Z), barley (Hordeum vulgare P), and garlic (Allium sativum A) were the most commonly used HM. Statistical analysis showed that consumers of HM had a higher rate of caesarian section and complications during and after the delivery.Conclusion: Consumption of HM during pregnancy was high including contraindicated modalities such as cinnamon (C. verum L) and barley (H. vulgare P). The use of potentially harmful modalities and association with complications pose a threat to the well-being of mothers and newborns. Further studies in this area will be helpful to generate evidence for the rational use of HM during pregnancy.
Urinary Aromatic Amino Acid Metabolites Associated With Postoperative Emergence Agitation in Paediatric Patients After General Anaesthesia: Urine Metabolomics Study
Authors: Li Yueyue , Li Jingjie , Shi Yuhuan , Zhou Xuhui , Feng Wanqing , Han Lu , Ma Daqing , Jiang Hong , Yuan Yongfang 
Published: July 2022

Background: Emergence agitation (EA) is very common in paediatric patients during recovery from general anaesthesia, but underlying mechanisms remain unknown. This prospective study was designed to profile preoperative urine metabolites and identify potential biomarkers that can predict the occurrence of EA.Methods: A total of 224 patients were screened for recruitment; of those, preoperative morning urine samples from 33 paediatric patients with EA and 33 non-EA gender- and age-matched patients after being given sevoflurane general anaesthesia were analysed by ultra-high-performance liquid chromatography (UHPLC) coupled with a Q Exactive Plus mass spectrometer. Univariate analysis and orthogonal projection to latent structures squares-discriminant analysis (OPLS-DA) were used to analyse these metabolites. The least absolute shrinkage and selection operator (LASSO) regression was used to identify predictive variables. The predictive model was evaluated through the receiver operating characteristic (ROC) analysis and then further assessed with 10-fold cross-validation.Results: Seventy-seven patients completed the study, of which 33 (42.9%) patients developed EA. EA and non-EA patients had many differences in preoperative urine metabolic profiling. Sixteen metabolites including nine aromatic amino acid metabolites, acylcarnitines, pyridoxamine, porphobilinogen, 7-methylxanthine, and 5′-methylthioadenosine were found associated with an increased risk of EA, and they all exhibited higher levels in the EA group than in the non-EA group. The main metabolic pathways involved in these metabolic changes included phenylalanine, tyrosine and tryptophan metabolisms. Among these potential biomarkers, L-tyrosine had the best predictive value with an odds ratio (OR) (95% CI) of 5.27 (2.20–12.63) and the AUC value of 0.81 (0.70–0.91) and was robust with internal 10-fold cross-validation.Conclusion: Urinary aromatic amino acid metabolites are closely associated with EA in paediatric patients, and further validation with larger cohorts and mechanistic studies is needed.Clinical Trial Registration:clinicaltrials.gov, identifier NCT04807998
Comparative Pharmacokinetics of Cinobufacini Capsule and Injection by UPLC-MS/MS
Authors: Li Ming , Qin Yanhong , Li Zhe , Lan Jinshuai , Zhang Tong , Ding Yue 
Published: July 2022

Cinobufacini capsule and injection are two different formulations from the same source, obtained from the extraction of the skin of Bufo bufo gargarizans Cantor, which have been approved by the Chinese State Food and Drug Administration (CFDA) for the treatment of various cancers. Our previous study has found that the cinobufacini capsule and injection exhibited different anticancer effects, but their different pharmacokinetic behaviors, which could give a cause of that, have never been reported. So a sensitive and selective method for the simultaneous quantitation of 13 compounds in the rat plasma, including bufothionine, hellebrigenin, bufalin, gamabufotalin, telocinobufagin, cinobufagin, arenobufagin, cinobufotalin, desacetylcinobufotalin, bufotalin, pseudobufarenogin, resibufogenin, and desacetylcinobufagin, was established by using the Agilent 6460 mass spectrometer equipped with an ESI ion source in a multiple-reaction monitoring (MRM) mode. Chromatographic analysis was accomplished in 6 min by using an Agilent SB-C18 column and a mobile phase consisting of 0.1% formic acid in water and acetonitrile in an optimized gradient program at a flow rate of 0.3 ml/min. The correlation coefficients (r) of all analytes ranged from 0.9967 to 0.9996, while their lower limits of quantification ranged from 0.20 to 4.84 ng/ml. The method has been fully verified and applied for the pharmacokinetic difference study of the Cinobufacini capsule and injection in rats. The results showed that nine components could be quantitated in rat plasma samples after the administration of the cinobufacini capsule, while only bufothionine, bufalin, arenobufagin, and pseudobufarenogin could be detected in the cinobufacini injection group. Their pharmacokinetic studies indicated telocinobufagin, bufalin, desacetylcinobufagin, and arenobufagin were predicted as the potential active substances of the Cinobufacini capsule, while bufothionine was considered as a major ingredient in the cinobufacini injection due to its relatively high blood drug exposure. Also, the AUC of the nine components in cinobufacini capsule groups with three different doses showed a similar trend with significant differences, and the exposure increased with the increase of the dose. The pharmacokinetic characteristics of all major ingredients in cinobufacini capsules and injection were of wide variation, which could be used to explain differences in the efficacy of the cinobufacini capsule and injection and infer the pharmacodynamic ingredients of various cinobufacini preparations.
Association between TPH1 polymorphisms and the risk of suicide behavior: An updated meta-analysis of 18,398 individuals
Authors: Genis-Mendoza Alma Delia , Hernández-Díaz Yazmín , González-Castro Thelma Beatriz , Tovilla-Zárate Carlos Alfonso , Castillo-Avila Rosa Giannina , López-Narváez María Lilia , Ramos-Méndez Miguel Ángel , Nicolini Humberto 
Published: July 2022

ObjectivesWe aimed to examine the association ofTPH1polymorphisms with the risk of suicide behavior (SB).DesignSystematic review and meta-analysis.MethodAll relevant studies that evaluated the association between the A218C (rs1800532), A779C (rs1799913) and A6526G (rs4537731) polymorphisms and the susceptibility to SB published up to September 2021 were identified through a comprehensive systematic search in PubMed, Scopus, EBSCO and Science Direct electronic databases. The association betweenTPH1gene polymorphisms and SB was evaluated using inherence models by odds ratio (OR) and 95% confidence interval (CI). Subgroup analyses, heterogeneity analyses, and publication bias were also tested in this meta-analysis.ResultsThe meta-analysis forTPH1A218C revealed an increased risk of SB in the dominant model (OR = 1.11, 95%CI 1.01–1.22). We also observed a positive association in the allelic (OR = 1.13, 95%CI 1.05–1.21), homozygous (OR = 1.22, 95%CI 1.06–1.40), heterozygous (OR = 1.21, 95%CI 1.08–1.37) and dominant (OR = 1.21, 95%CI 1.09–1.34) inherence models with the suicide attempt. Additionally, in the heterozygous (OR = 0.84, 95%CI 0.73–0.97) and dominant (OR = 0.79, 95%CI 0.68–0.91) inherence models we detected an association with completed suicide. Based on ethnicity, an association of SB in the European population also was observed (OR = 1.29, 95%CI 1.12–1.51). However, for both A779C and A6526G polymorphisms we did not find evidence of an association with SB.ConclusionThis meta-analysis suggests that the A218C polymorphism ofTPH1gene could be a possible risk factor of SB. Future large-scale studies are required to analyze the molecular mechanisms by which affect the susceptibility of developing suicide behavior.
Mental health in individuals with self-reported psychiatric symptoms during the COVID-19 pandemic: Baseline data from a swedish longitudinal cohort study
Authors: Rozental Alexander , Sörman Karolina , Ojala Olivia , Jangard Simon , El Alaoui Samir , Månsson Kristoffer N. T. , Shahnavaz Shervin , Lundin Johan , Forsström David , Hedman-Lagerlöf Maria , Lundgren Tobias , Jayaram-Lindström Nitya 
Published: July 2022

ObjectiveIndividuals with psychiatric disorders may be both vulnerable and sensitive to rapid societal changes that have occurred during the COVID-19 pandemic. To fully understand these impacts, repeated measurements of these individuals are warranted. The current longitudinal study set out to perform monthly assessment of individuals with common psychiatric disorders using established questionnaires with a possibility for them to self- rate their symptoms, over time.MethodsRecruitment of individuals who identified themselves as struggling with mental health problems, living in Sweden between July 2020 and June 2021 using an online survey. The individuals answered questions on demographics, psychiatric history, current psychiatric symptoms (e.g., Patient Health Questionnaire, PHQ-9; General Anxiety Disorder, GAD-7), somatic health, health-care contacts and any changes therein during the pandemic. Monthly, longitudinal assessments are still ongoing (consenting participants provide data for 1 year), and here we present descriptive statistics from the baseline measurement. All measurements from baseline (&gt;400 items), and follow-ups are presented in detail.ResultsA total of 6.095 participants (average age 35 years) submitted complete baseline data. Marital status (43% single) and number of years of education (48% highest degree being high school) were evenly distributed in this population. The most common lifetime psychiatric disorder in the sample was depressive disorder (80.5%) and generalized anxiety disorder (45.9%), with a substantial proportion having severe symptoms of depression. (30.5%) and anxiety (37.1%). Lifetime suicidal ideation (75.0%) and non-suicidal self-harm (57.7%) were prevalent in the group and 14.5% reported drug use during the pandemic. Allergies (36.8%) were the most common somatic condition, followed by irritable bowel syndrome (18.7%). For those having experienced a traumatic event, 39% showed symptoms during the pandemic indicating PTSD. Regarding contact with mental health services during the pandemic, 22% had established a new contact, and 20% reported to have increased their psychiatric medication compared to before the pandemic.ConclusionBaseline data collected during the pandemic from individuals in Sweden with pre-existing psychiatric disorders demonstrate that this sample represents a population suitable for an investigation on the long-term impact of the pandemic, as intended by the longitudinal investigation that is ongoing. Follow-up questionnaires over a 12-month period are being collected and will indicate how the health and well-being of this population was impacted during the changes and uncertainties that have been characteristic of the past 2 years.
Self-reported side effects of the Oxford AstraZeneca COVID-19 vaccine among healthcare workers in Ethiopia, Africa: A cross-sectional study
Authors: Yesuf Elias Ali , Riad Abanoub , Sofi-Mahmudi Ahmad , Sudhakar Morankar , Mekonnen Addisalem , Endalkachew Selamawit , Mama Feyissa , Muhidin Semira , Ayele Bethelhem , Yahya Mohammed , Usman Abduselam , Abafita Jemal , Klugar Miloslav 
Published: July 2022

IntroductionEthiopia is the second most populous country in Africa. Ethiopia received most of its COVID-19 vaccines through donations. The Oxford AstraZeneca vaccine is the first to be donated to Ethiopia by the COVAX facility. Healthcare workers were the priority population that received the Oxford AstraZeneca COVID-19 vaccine. However, there was no nationwide study on the safety of the vaccine in Ethiopia. This study aimed to measure the prevalence and predictors of self-reported side effects of the Oxford AstraZeneca vaccine.Materials and methodsThe study employed a cross-sectional design. A sample of healthcare workers who took Oxford AstraZeneca COVID-19 vaccine was drawn from four regions of Ethiopia; namely, Amhara, Oromia, Somali, and Southwest. Data were collected on sociodemographic characteristics, medical anamnesis, COVID-19 related anamnesis, and COVID-19 vaccine anamnesis via telephone interview. Descriptive and inferential analyses were done. The software, IBM SPSS Statistics v21.0, was used for analyses of data.ResultsOut of 384 people, 346 responded (response rate: 90.1%). Female accounted for 34.1% of the respondents. The mean age of the respondents was 31.0 years (Standard Deviation (SD) = 7.4). Nurses accounted for 43.7% of the respondents. The prevalence of at least one local- and systemic-side effect was 50.6 and 44.5%, respectively. The most frequent local- and systemic- side effect were injection site pain and headache, respectively. Both types of side effects mostly subsided in the first 3 days. A third of healthcare workers with side effects took at least one medication. Paracetamol followed by diclofenac sodium were taken by healthcare workers to overcome side effects. There was no independent predictor of local side effect. After controlling for age and chronic diseases, the odds of healthcare workers with COVID-19 like symptoms to experience systemic side effects was 1.38 (Confidence Interval (CI): 1.04–1.82) times more than that of healthcare workers without COVID-19 like symptoms.ConclusionsThe prevalence of local- and systemic-side effects of the Oxford AstraZeneca COVID-19 vaccine was modest. As the symptoms were mostly common in the first 3 days, it is preferable to monitor healthcare workers at least in the first 3 days following the administration of the vaccine.
Improvement of Molecular Genetic Diagnostics of Infections Caused by Mycoplasma Pneumoniae and Chlamydia Pneumoniae
Authors: Глинкина Т.В. 
Published: July 2022

Цель. Усовершенствовать метод выявления ДНК Mycoplasma pneumoniae и Сhlamydia pneumoniae с использованием полимеразной цепной реакции в режиме реального времени на основе выбора оригинальных праймеров и проб для качественной, количественной и дифференциальной диагностики инфекций.Материалы и методы. Методологический подход включал выбор специфических последовательностей из геномов Mycoplasma pneumoniae и Сhlamydia pneumoniae для дизайна праймеров и проб, создание положительного контрольного образца, подбор условий амплификации; апробация метода проведена на образцах ДНК, выделенных из соскобов эпителиальных клеток из полости носа, ротоглотки и мокроты детей с бронхитами и пневмониями.Результаты. Усовершенствован метод на основе мультиплексной полимеразной цепной реакции в режиме реального времени, характеризующийся высокой аналитической чувствительностью – 10 копий ДНК Mycoplasma pneumoniae и Сhlamydia pneumoniae на реакцию, и специфичностью, составившей 100%.Выводы. Данный метод позволяет одновременно проводить выявление ДНК Mycoplasma pneumoniae и Сhlamydia pneumoniae и определять концентрацию ДНК Mycoplasma pneumoniae в мокроте.
Purpose. Optimize the detection of Mycoplasma pneumoniae and Сhlamydia pneumoniae by real-time polymerase chain reaction on the base of the choice of original primers and probes for qualitative, quantitative and differential diagnostics of infections.Materials and methods. The methodological approach included the development of primers and probes specific for genome sequences of Mycoplasma pneumoniae and Сhlamydia pneumoniae, positive control DNA template, polymerase chain reaction conditions; the method was tested on DNA samples isolated from nasopharyngeal swabs and sputum of children with bronchitis and pneumonia.Results. The optimized method is based on the real-time multiplex polymerase chain reaction, the method’s analytical sensitivity is 10 copies of each Mycoplasma pneumoniae and Chlamydia pneumoniae DNA per reaction, and specificity – 100%.Conclusions. The optimized method gives the possibility to detect simultaneously Mycoplasma pneumoniae and Chlamydia pneumoniae DNA and determine the concentration of Mycoplasma pneumoniae DNA in sputum.
Clinical Significance of the Study of the Association of Allelic Polymorphism of Genes as Predictors of the Development and Unfavorable Course of Sepsis (Analytical Review of the Literature)
Authors: Сергиенко Е.Н. , Романова О.Н. , Фомина Е.Г. , Григорьева Е.Е. , Зверко В.В. 
Published: July 2022

Сепсис представляет собой осложненное развитием бактериальной инфекции проявление синдрома системного воспалительного ответа, течение которого сопровождается угрожающей жизни органной дисфункцией, вызванной особенностями реакции иммунного ответа на инфекцию. Несмотря на современные стратегии реанимации и достижения в медицине, сепсис и его последствия остаются частой причиной смерти пациентов с внебольничными и внутрибольничными инфекциями. Поэтому для его ранней диагностики и оказания своевременной лечебно-диагностической помощи пациентам требуется использование информативных диагностических маркеров с целью выявления пациентов с высоким риском заболевания. Все больше данных свидетельствуют о том, что генетические вариации, особенно однонуклеотидные полиморфизмы (SNP) в целом ряде генов, ответственных за врожденный иммунитет, могут влиять на риск развития у пациентов серьезной инфекции. Определение различий в генах и связанных с ними различий в реакции на сепсис может способствовать разработке новых генетически адаптированных диагностических и терапевтических вмешательств для улучшения результатов лечения пациентов с восприимчивостью к сепсису.
Sepsis is a pathological condition with the development of life-threatening organ dysfunction caused by a violation of the regulation of the immune response to infection. Despite modern resuscitation strategies and advances in medicine, sepsis and its consequences remain a common cause of death in patients with community-acquired and nosocomial infections. Therefore, for its early diagnosis and timely care, prognostic markers are needed to identify high-risk patients. More and more evidence suggests that genetic variations, especially single nucleotide polymorphisms (SNPs) in a number of genes responsible for innate immunity, may affect the risk of serious infection in patients. The identification of differences in genes and related differences in response to sepsis may contribute to the development of new genetically adapted diagnostic and therapeutic interventions to improve outcomes if treatment in patients with sepsis susceptibility.