bluuebunny/crossref_metadata_2025_split huggingface dataset:
Facile and scalable tubing-free sample loading for droplet microfluidics
Authors: Shao Fangchi , Hsieh Kuangwen , Zhang Pengfei , Kaushik Aniruddha M. , Wang Tza-Huei 
Published: August 2022

AbstractDroplet microfluidics has in recent years found a wide range of analytical and bioanalytical applications. In droplet microfluidics, the samples that are discretized into droplets within the devices are predominantly loaded through tubings, but such tubing-based sample loading has drawbacks such as limited scalability for processing many samples, difficulty for automation, and sample wastage. While advances in autosamplers have alleviated some of these drawbacks, sample loading that can instead obviate tubings offers a potentially promising alternative but has been underexplored. To fill the gap, we introduce herein a droplet device that features a new Tubing Eliminated Sample Loading Interface (TESLI). TESLI integrates a network of programmable pneumatic microvalves that regulate vacuum and pressure sources so that successive sub-microliter samples can be directly spotted onto the open-to-atmosphere TESLI inlet, vacuumed into the device, and pressurized into nanoliter droplets within the device with minimal wastage. The same vacuum and pressure regulation also endows TESLI with cleaning and sample switching capabilities, thus enabling scalable processing of many samples in succession. Moreover, we implement a pair of TESLIs in our device to parallelize and alternate their operation as means to minimizing idle time. For demonstration, we use our device to successively process 44 samples into droplets—a number that can further scale. Our results demonstrate the feasibility of tubing-free sample loading and a promising approach for advancing droplet microfluidics.
Cross-cultural adaptation and psychometric properties of the Spanish Quality in Psychiatric Care Forensic Inpatient Staff (QPC-FIPS) instrument
Authors: Domínguez del Campo Marta , Moreno-Poyato Antonio R. , Puig-Llobet Montserrat , Lluch-Canut Maria Teresa , Rodríguez Zunino Nathalia , Tomás-Jiménez Manuel , Sanchez-Balcells Sara , Schröder Agneta , Lundqvist Lars-Olov , Escuder-Romeva Gemma , Roldán-Merino Juan 
Published: August 2022

Abstract"Quality in Psychiatric Care-Forensic Inpatient Staff (QPC-FIPS) is an instrument of Swedish origin validated to measure the perception of the quality of mental health care provided by forensic psychiatry professionals. The aim of this study was to cross-culturally adapt the QPC-FIPS instrument and to evaluate the psychometric properties of the Spanish version of the instrument. A psychometric study was carried out. For validity, content validity, convergent validity and construct validity were included. For reliability, the analysis of internal consistency and temporal stability was included. The sample consisted of 153 mental health professionals from four Forensic Psychiatry units. The adapted Spanish version of the QPC-FIPS scale was configured with the same number of items and dimensions as the original. The psychometric properties, in terms of temporal stability and internal consistency, were adequate and the factor structure, such as the homogeneity of the dimensions of the Spanish version of the QPC-FIPS, was equivalent to the original Swedish version. We found that the QPC_FIPS-Spanish is a valid, reliable and easy-to-apply instrument for assessing the self-perception of professionals regarding the care they provide.
Establishing a low-risk zone for a temporary extra-articular calcaneo-tibial pin fixation in an unstable ankle or subtalar joint
Authors: Yang Ik , Lee Ho Won , Xu Huiying , Kang Seung Rim , Kim Hyong Nyun 
Published: August 2022

AbstractThis study aimed to establish a low-risk zone to avoid neurovascular injury during a temporary extra-articular calcaneo-tibial pin fixation in an unstable ankle or subtalar joint. A line from the calcaneal tuberosity center to the lateral end of the posterior malleolus at the ankle joint level defines the lateral border of this zone. Another line from the calcaneal tuberosity center to the midpoint of the anterior distal tibial articular surface at the joint level defines its medial border. This region was assumed to have a low neurovascular injury risk upon pin insertion. Fifty ankles from 50 patients who had undergone magnetic resonance imaging (MRI) for ankle disorders were assessed. T1-weighted oblique axial MRI slices were oriented to the pin trajectory. The mean distances between the sural nerve and the lateral border of the low-risk zone and between the posterior tibial neurovascular structures and the medial border of the low-risk zone were 15.0 ± 2.5 (range 9.1 to 21.1) and 12.8 ± 2.6 (6.3 to 20.8) mm, respectively. No neurovascular structures were identified within the low-risk zone. These findings demonstrated that an unstable ankle or subtalar joint can be temporarily fixated with an extra-articular calcaneo-tibial pin at a defined zone with a low neurovascular injury risk.
ADAR3 activates NF-κB signaling and promotes glioblastoma cell resistance to temozolomide
Authors: Raghava Kurup Reshma , Oakes Eimile K. , Vadlamani Pranathi , Nwosu Obi , Danthi Pranav , Hundley Heather A. 
Published: August 2022

AbstractThe RNA binding protein ADAR3 is expressed exclusively in the brain and reported to have elevated expression in tumors of patients suffering from glioblastoma compared to adjacent brain tissue. Yet, other studies have indicated that glioblastoma tumors exhibit hemizygous deletions of the genomic region encompassing ADAR3 (10p15.3). As the molecular and cellular consequences of altered ADAR3 expression are largely unknown, here we directly examined the impacts of elevated ADAR3 in a glioblastoma cell line model. Transcriptome-wide sequencing revealed 641 differentially expressed genes between control and ADAR3-expressing U87-MG glioblastoma cells. A vast majority of these genes belong to pathways involved in glioblastoma progression and are regulated by NF-κB signaling. Biochemical and molecular analysis indicated that ADAR3-expressing U87-MG cells exhibit increased NF-κB activation, and treatment with an NF-κB inhibitor abrogated the impacts of ADAR3 on gene expression. Similarly, we found that increased cell survival of ADAR3-expressing cells to temozolomide, the preferred chemotherapeutic for glioblastoma, was due to increased NF-κB activity. Aberrant constitutive NF-κB activation is a common event in glioblastoma and can impact both tumor progression and resistance to treatment. Our results suggest that elevated ADAR3 promotes NF-κB activation and a gene expression program that provides a growth advantage to glioblastoma cells.
The impact of alkaline treatments on elasticity in spruce tonewood
Authors: Malvermi Raffaele , Albano Michela , Gonzalez Sebastian , Fiocco Giacomo , Antonacci Fabio , Malagodi Marco , Sarti Augusto 
Published: August 2022

AbstractIt is commonly believed that violins sound differently when finished. However, if the role of varnishes on the vibrational properties of these musical instruments is well-established, how the first components of the complete wood finish impact on the final result is still unclear. According to tradition, the priming process consists of two distinct stages, called pre-treatment and sizing. The literature reports some recipes used by old Cremonese luthiers as primers, mainly based on alkaline aqueous solutions and protein-based glues. In this manuscript, we analyze the impact of these treatments on the mechanical properties of the material. The combination of two pre-treatments and three sizes is considered on nine different plates. We compare the vibrational properties before and after the application and assess the effects of the different primers, also supported by finite element modeling. The main outcome is that the combination of particular treatments on the violin surface before varnishing leads to changes not only to the wood appearance, but also to its vibrational properties. Indeed pre-treatments, often considered negligible in terms of vibrational changes, enhance the penetration of the size into the wood structure and strengthen the impact of the latter on the final rigidity of the material along the longitudinal and radial directions.
Long-term follow-up MRI shows no hastening of adjacent segment degeneration following cervical disc arthroplasty
Authors: Burkhardt Benedikt W. , Baumann Lukas , Simgen Andreas , Wagenpfeil Gudrun , Hendrix Philipp , Reith Wolfgang , Oertel Joachim M. 
Published: August 2022

AbstractCervical disc arthroplasty is an established procedure, but studies with data on long-term clinical outcome, reoperation for symptomatic adjacent segment degeneration (sASD), and degenerative changes based on MRI findings are rare. Thus, a file review was performed and patients with complete documentation of neurological status at preoperative, postoperative, 12 month, 3–4 years follow-up including surgical reports for reoperation with a minimum follow-up of 9 years were included. Final follow-up assessment included a physical examination, assessment of pain levels, Odoms criteria, Neck disability index. The degeneration of each cervical segment at preoperative and at final follow-up was assessed using an MRI. Forty-six out of 68 included patients participated, the mean follow-up was 11 (range 9–15) years, at which 71.7% of patients were free of arm pain, 52.2% of patients were free of neck pain, 63% of patients had no sensory dysfunction, and full motor strength was noted in 95.6% of patients. The clinical success rate was 76.1%, the mean NDI was 12%. Overall repeated procedure rate was 17%, the reoperation rate for sASD was 9%, and removal of CDA was performed in 4%. MRI showed progressive degeneration but no significant changes of SDI from preoperative to final follow-up.
Direct constraint control for EM-based miniaturization of microwave passives
Authors: Koziel Slawomir , Pietrenko-Dabrowska Anna 
Published: August 2022

AbstractHandling constraints imposed on physical dimensions of microwave circuits has become an important design consideration over the recent years. It is primarily fostered by the needs of emerging application areas such as 5G mobile communications, internet of things, or wearable/implantable devices. The size of conventional passive components is determined by the guided wavelength, and its reduction requires topological modifications, e.g., transmission line folding, or utilization of compact cells capitalizing on the slow-wave phenomenon. The resulting miniaturized structures are geometrically complex and typically exhibit strong cross coupling effects, which cannot be adequately accounted for by analytical or equivalent network models. Consequently, electromagnetic (EM)-driven parameter tuning is necessary, which is computationally expensive. When the primary objective is size reduction, the optimization task becomes far more challenging due to the presence of constraints related to electrical performance figures (bandwidth, power split ratio, etc.), which are all costly to evaluate. A popular solution approach is to utilize penalty functions. Therein, possible violations of constraints degrade the primary objective, thereby enforcing their satisfaction. Yet, the appropriate setup of penalty coefficients is a non-trivial problem by itself, and is often associated to extra computational expenses. In this work, we propose an explicit approach to constraint handling, which is combined with the trust-region gradient-search procedure. In our technique, the decision about the adjustment of the search radius is determined based on the reliability of rendering the feasible region boundary by linear approximation models of the constraints. Comprehensive numerical experiments conducted using three miniaturized coupler structures demonstrate superiority of the presented method over the penalty function paradigm. Apart from the efficacy, its appealing features include algorithmic simplicity, and no need for tailoring the procedure for a particular circuit to be optimized.
DNAzyme recognition triggered cascade signal amplification for rapid and highly sensitive visual detection of uranyl ions
Authors: Zhang Ling , Shi Siwei , Xiong Penghui , Chen Lumin , Xu Jie , Jiang Jiaolai , Yang Shanli , Wu Haoxi 
Published: August 2022

A 40 min rapid and highly sensitive assay for visualized detection of UO22+ in water samples is reported. A dynamic range 1~50 nM and a LOD of 0.48 nM were obtained. Concentrations as low as 5 nM UO22+ could be distinguished by the naked eye.
Improving DFIG performance under fault scenarios through evolutionary reinforcement learning based control
Authors: Gao Wei , Fan Rui , Huang Renke , Huang Qiuhua , Du Yan , Qiao Wei , Wang Shaobu , Gao David Wenzhong 
Published: August 2022

AbstractThe doubly fed induction generator (DFIG) usually experiences high rotor current and DC capacitor link voltage spikes during system fault events. In this paper, a novel data‐driven approach is proposed to enhance DFIG performance under fault scenarios. An advanced reinforcement learning algorithm called guided surrogate‐gradient‐based evolution strategy (GSES) is used to control the DFIG power and capacitor DC‐link voltage by adjusting the optimal reference signals. This controller is able to prevent the DFIG rotor from over‐current risk and maintain grid‐connected operation. The proposed GSES‐based control algorithm was evaluated through simulations on a 3.6‐MW DFIG in the PSCAD/EMTDC software. Results have validated the effectiveness of the proposed GSES‐based control algorithm in improving DFIG performance under various fault scenarios.
Fault diagnosis technologies for power transformers during the on‐site inductive oscillating switching impulse voltage withstand test
Authors: Zhang Xuanrui , Wang Haotian , Guo Ruochen , Zhang Zheming , Li Junhao , Han Xutao 
Published: August 2022

AbstractAlthough oscillating switching impulse (OSI) voltage is recommended for the transformer field test, the lack of corresponding fault diagnosis technologies limits its application in the field. In this study, the inductive OSI test technology and diagnostic methods are investigated. The front time and the oscillation frequency of the OSI are altered by changing the additional inductance, whereas the tail time is affected by both the front and tail resistance. The OSI voltage withstand test is carried out on a 110 kV three‐phase double‐winding transformer on site. Furthermore, transfer function (TF) analysis is used for the diagnosis of transformer insulation with high sensitivity during the OSI test. The fault winding is distinguished by comparing the change in the voltage TF and current TF at different voltage levels. The partial discharge (PD) detection method has the highest sensitivity, which realizes the early diagnosis of insulation faults. The insulation status of the windings is ascertained based on the number and amplitude of PDs. The test results prove that the inductive OSI voltage withstand test is easy to perform on site and can successfully diagnose the insulation faults of the transformer in more detail compared with other routine insulation tests.
Measuring chemical abundances in AGN from infrared nebular lines: HII-CHI-MISTRY-IR for AGN
Authors: Pérez-Díaz Borja , Pérez-Montero Enrique , Fernández-Ontiveros Juan A. , Vílchez José M. 
Published: August 2022

Context. Future and ongoing infrared and radio observatories such as JWST, METIS, and ALMA will increase the amount of rest-frame IR spectroscopic data for galaxies by several orders of magnitude. While studies of the chemical composition of the interstellar medium (ISM) based on optical observations have been widely spread over decades for star-forming galaxies (SFGs) and, more recently, for active galactic nuclei (AGN), similar studies need to be performed using IR data. In the case of AGN, this regime can be especially useful given that it is less affected by temperature and dust extinction, traces higher ionic species, and can also provide robust estimations of the chemical abundance ratio N/O.
Aims. We present a new tool based on a Bayesian-like methodology (HII-CHI-MISTRY-IR) to estimate chemical abundances from IR emission lines in AGN. We use a sample of 58 AGN with IR spectroscopic data retrieved from the literature, composed by 43 Seyferts, eight ultraluminous infrared galaxies (ULIRGs), four luminous infrared galaxies (LIRGs), and three low-ionization nuclear emission line regions (LINERs), to probe the validity of our method. The estimations of the chemical abundances based on IR lines in our sample are later compared with the corresponding abundances derived from the optical emission lines in the same objects.
Methods. HII-CHI-MISTRY-IR takes advantage of photoionization models, characterized by the chemical abundance ratios O/H and N/O, and the ionization parameter U, to compare their predicted emission-line fluxes with a set of observed values. Instead of matching single emission lines, the code uses some specific emission-line ratios that are sensitive to the above free parameters.
Results. We report mainly solar and also subsolar abundances for O/H in the nuclear region for our sample of AGN, whereas N/O clusters are around solar values. We find a discrepancy between the chemical abundances derived from IR and optical emission lines, the latter being higher than the former. This discrepancy, also reported by previous studies of the composition of the ISM in AGN from IR observations, is independent of the gas density or the incident radiation field to the gas, and it is likely associated with dust obscuration and/or temperature stratification within the gas nebula.
Millimeter wave spectrum and search for vinyl isocyanate toward Sgr B2(N) with ALMA
Authors: Vávra K. , Kolesniková L. , Belloche A. , Garrod R. T. , Koucký J. , Uhlíková T. , Luková K. , Guillemin J.-C. , Kania P. , Müller H. S. P. , Menten K. M. , Urban Š. 
Published: August 2022

Context. The interstellar detections of isocyanic acid (HNCO), methyl isocyanate (CH3NCO), and very recently also ethyl isocyanate (C2H5NCO) invite the question of whether or not vinyl isocyanate (C2H3NCO) can be detected in the interstellar medium. There are only low-frequency spectroscopic data (<40 GHz) available for this species in the literature, which makes predictions at higher frequencies rather uncertain, which in turn hampers searches for this molecule in space using millimeter (mm) wave astronomy.
Aims. The aim of the present study is on one hand to extend the laboratory rotational spectrum of vinyl isocyanate to the mm wave region and on the other to search, for the first time, for its presence in the high-mass star-forming region Sgr B2, where other isocyanates and a plethora of complex organic molecules are observed.
Methods. We recorded the pure rotational spectrum of vinyl isocyanate in the frequency regions 127.5–218 and 285–330 GHz using the Prague mm wave spectrometer. The spectral analysis was supported by high-level quantum-chemical calculations. On the astronomy side, we assumed local thermodynamic equilibrium to compute synthetic spectra of vinyl isocyanate and to search for it in the ReMoCA survey performed with the Atacama Large Millimeter/submillimeter Array (ALMA) toward the high-mass star-forming protocluster Sgr B2(N). Additionally, we searched for the related molecule ethyl isocyanate in the same source.
Results. Accurate values for the rotational and centrifugal distortion constants are reported for the ground vibrational states of trans and cis vinyl isocyanate from the analysis of more than 1000 transitions. We report nondetections of vinyl and ethyl isocyanate toward the main hot core of Sgr B2(N). We find that vinyl and ethyl isocyanate are at least 11 and 3 times less abundant than methyl isocyanate in this source, respectively.
Conclusions. Although the precise formation mechanism of interstellar methyl isocyanate itself remains uncertain, we infer from existing astrochemical models that our observational upper limit for the CH3NCO:C2H5NCO ratio in Sgr B2(N) is consistent with ethyl isocyanate being formed on dust grains via the abstraction or photodissociation of an H atom from methyl isocyanate, followed by the addition of a methyl radical. The dominance of such a process for ethyl isocyanate production, combined with the absence of an analogous mechanism for vinyl isocyanate, would indicate that the ratio C2H3NCO:C2H5NCO should be less than unity. Even though vinyl isocyanate was not detected toward Sgr B2(N), the results of this work represent a significant improvement on previous low-frequency studies and will help the astronomical community to continue searching for this species in the Universe.
Detecting chiral asymmetry in the interstellar medium using propylene oxide
Authors: Lankhaar Boy 
Published: August 2022

Context.Life is distinctly homochiral. The origins of this homochirality are under active debate. Recently, propylene oxide has been detected in the gas-phase interstellar medium (ISM). The enantiomeric composition of ISM propylene oxide may be probed through circular polarization measurements, but accurate estimates of the circular dichroism properties of the microwave transitions of propylene oxide are not available.Aims.Our aim is to develop a model of the circular dichroic activity in torsion–rotation transitions of closed-shell chiral molecules such as propylene oxide. With this model we can estimate the viability, and optimize the observation strategies, of enantiomeric excess detection in ISM propylene oxide.Methods.Circular dichroism in spectral lines manifests through the simultaneous interaction of an electromagnetic radiation field with the molecular electric dipole moment and magnetic dipole moment. We developed techniques to quantify electric dipole and magnetic dipole moments of torsion–rotation transitions by expanding on earlier modeling of the electric and magnetic dipole properties of single torsion–rotation levels. To model the circular dichroism properties of propylene oxide, we used these techniques in combination with ab initio quantum chemical calculations.Results.The expressions for the dichroic activity of the microwave transitions of torsionally active molecules are derived. We find that the torsional motion of molecules exhibiting internal rotation contributes significantly to the total magnetic moment. We present estimates for the dichroic activity of the torsion–rotation transitions of propylene oxide. We predict that the circular polarization fractions of emission lines of enantiopure propylene oxide relevant to astronomical detections are on the order of 10−6.Conclusions.Due to the low predicted circular polarization fractions, we conclude that enantiomeric characterization of propylene oxide in the gas phase of the ISM is impossible with the current astronomical observation techniques. We suggest that only chiral radical species may be viably employed for purposes of enantiomeric excess detection. We estimate that laboratory experiments may be successful in detecting the enantiomeric composition of a mixture of propylene oxide through microwave dichroism spectroscopy.