bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation
Authors: Atkinson Eliza , Tuza Zoltan , Perrino Giansimone , Stan Guy-Bart , Ledesma-Amaro Rodrigo 
Published: June 2022

Abstract
Background
Low-cost sustainable feedstocks are essential for commercially viable biotechnologies. These feedstocks, often derived from plant or food waste, contain a multitude of different complex biomolecules which require multiple enzymes to hydrolyse and metabolise. Current standard biotechnology uses monocultures in which a single host expresses all the proteins required for the consolidated bioprocess. However, these hosts have limited capacity for expressing proteins before growth is impacted. This limitation may be overcome by utilising division of labour (DOL) in a consortium, where each member expresses a single protein of a longer degradation pathway.

Results
Here, we model a two-strain consortium, with one strain expressing an endohydrolase and a second strain expressing an exohydrolase, for cooperative degradation of a complex substrate. Our results suggest that there is a balance between increasing expression to enhance degradation versus the burden that higher expression causes. Once a threshold of burden is reached, the consortium will consistently perform better than an equivalent single-cell monoculture.

Conclusions
We demonstrate that resource-aware whole-cell models can be used to predict the benefits and limitations of using consortia systems to overcome burden. Our model predicts the region of expression where DOL would be beneficial for growth on starch, which will assist in making informed design choices for this, and other, complex-substrate degradation pathways.
Nanoparticles mediated tumor microenvironment modulation: current advances and applications
Authors: Raju Ganji Seeta Rama , Pavitra Eluri , Varaprasad Ganji Lakshmi , Bandaru Sai Samyuktha , Nagaraju Ganji Purnachandra , Farran Batoul , Huh Yun Suk , Han Young-Kyu 
Published: June 2022

AbstractThe tumor microenvironment (TME) plays a key role in cancer development and emergence of drug resistance. TME modulation has recently garnered attention as a potential approach for reprogramming the TME and resensitizing resistant neoplastic niches to existing cancer therapies such as immunotherapy or chemotherapy. Nano-based solutions have important advantages over traditional platform and can be specifically targeted and delivered to desired sites. This review explores novel nano-based approaches aimed at targeting and reprogramming aberrant TME components such as macrophages, fibroblasts, tumor vasculature, hypoxia and ROS pathways. We also discuss how nanoplatforms can be combined with existing anti-tumor regimens such as radiotherapy, immunotherapy, phototherapy or chemotherapy to enhance clinical outcomes in solid tumors.
Preliminary evaluation of the Chinese version of the patient-reported outcomes measurement information system 29-item profile in patients with aortic dissection
Authors: Huang Wanbing , Wu Qiansheng , Zhang Yufen , Tian Chong , Huang Haishan , Huang Sufang , Zhou Yanrong , He Jing , Wang Hui 
Published: June 2022

Abstract
Background
The Patient-Reported Outcomes Measurement Information System 29-item Profile (PROMIS-29) has been widely used to measure health outcomes from the patient’s perspective. It has not been validated in adults with aortic disease. The aim of this study was to explore the reliability and validity of the Chinese PROMIS-29 among patients undergoing surgery for aortic dissection (AD).

Methods
A cross-sectional design was applied. Eligible patients completed a questionnaire that contained the PROMIS-29 and legacy measures, including the Short Form-12 Health Survey (SF-12), 8-item Somatic Symptom Scale (SSS-8), Generalized Anxiety Disorder–2 (GAD-2), and Patient Health Questionnaire-2 (PHQ-2). The structural validity of the PROMIS-29 was evaluated using confirmatory factor analysis (CFA). Reliability was evaluated with Cronbach’s α. Construct validity was assessed by calculating Spearman’s rank correlations and comparing known-group differences.

Results
In total, a sample of 327 AD patients was included in the final analysis. Most of them were male (89%) with a mean age of 52.7 (± 10.3). CFA revealed good model fit of the seven-factor structure within PROMIS-29, as well as most domains in single-factor analysis. Reliability was confirmed with Cronbach’s α > 0.90. Correlations between comparable domains of the PROMIS-29 and those of legacy questionnaires and most know-group comparisons were observed as hypothesized.

Conclusions
This study found evidence for acceptable structural validity, construct validity and internal consistency of the PROMIS-29 in a sample of AD patients. It can be applied to AD survivors by researchers or clinicians, measuring outcomes after surgery and identifying those with worse health status.
The Multifaceted Melanocortin Receptors
Authors: Laiho Linda , Murray Joanne Fiona 
Published: June 2022

AbstractThe 5 known melanocortin receptors (MCs) have established physiological roles. With the exception of MC2, these receptors can behave unpredictably, and since they are more widely expressed than their established roles would suggest, it is likely that they have other poorly characterized functions. The aim of this review is to discuss some of the less well-explored aspects of the 4 enigmatic members of this receptor family (MC1,3-5) and describe how these are multifaceted G protein–coupled receptors (GPCRs). These receptors appear to be promiscuous in that they bind several endogenous agonists (products of the proopiomelanocortin [POMC] gene) and antagonists but with inconsistent relative affinities and effects. We propose that this is a result of posttranslational modifications that determine receptor localization within nanodomains. Within each nanodomain there will be a variety of proteins, including ion channels, modifying proteins, and other GPCRs, that can interact with the MCs to alter the availability of receptor at the cell surface as well as the intracellular signaling resulting from receptor activation. Different combinations of interacting proteins and MCs may therefore give rise to the complex and inconsistent functional profiles reported for the MCs. For further progress in understanding this family, improved characterization of tissue-specific functions is required. Current evidence for interactions of these receptors with a range of partners, resulting in modulation of cell signaling, suggests that each should be studied within the full context of their interacting partners. The role of physiological status in determining this context also remains to be characterized.
Toward Understanding the Photogenic New Citizen: Performance in Vernacular Photography from the Early Turkish Republic, 1920s–1940s
Authors: Baykan Calafato Özge 
Published: June 2022

Abstract
This portfolio looks at photographic self-representations of Turkish citizens of a newly established nation-state from the 1920s until the 1940s. The article explores the ways in which modern Turkish women and men performed their interpretation of the photogenic in photographic self-representations and how these self-representations negotiated the Kemalists' idea of modern citizenship in the early republican era. The desired selves performed in vernacular photographs were influenced not only by the Kemalist revolution but also by class aspirations of the citizens and wider sociocultural developments of the time. In addition, this portfolio reveals some of the complex circulation networks for photographic exchanges, as also suggested by inscriptions produced at the time, contributing to a deeper understanding as to how people constructed their interpretation of the photographic.
Revisiting the functionally generated path technique: is this an aid to predictable digital occlusal design? Part 2. The technique
Authors: Bereznicki Tom , Patel Rohit , Clark Megan 
Published: June 2022

The precision of the occlusal scheme is paramount to the production of a successful fixed restoration(s) and its long-term success. The provision of the correct occlusal scheme is one of the requirements when working conformatively, whether in analogue or digital format. Part 1 highlighted occlusal schemes that provide difficulties using established techniques in arriving at a predictable restoration requiring minimal occlusal adjustments chairside. Part 2 introduces the theory of the functionally generated path (FGP) technique. Two cases involving implant-retained restorations are used to highlight the benefit of adopting this technique into the digital workflow in the provision of occlusally precise restorations. CPD/Clinical Relevance: This article describes the value of the functionally generated path technique, when working digitally, for recording the occlusion for implant-retained restorations.
Managing early childhood caries with silver diamine fluoride in the COVID-19 context: case report
Authors: Cabrera Luis Raziel Martagón , Pacheco Fabio Gregorio Arriola 
Published: June 2022

COVID-19 is challenging modern dentistry, obliging all professionals to look for treatment strategies that are in accordance with health and ethical standards, so as to avoid virus transmission in the dental office. In recent years, paediatric dentistry has benefited from minimally invasive techniques to prevent and treat early childhood caries. Silver diamine fluoride (SDF) is a material that follows this philosophy because it provides professionals preventive and arresting actions for carious lesions in a practical and effective way. The present case details the management of a preschool-age patient who attended the dental office due to an emergency during the pandemic. The patient's emergency was resolved, but due to the diagnosis of severe early childhood caries, other mild and moderate carious, lesions were managed with SDF with the objective of avoiding future pulpal lesion development. The aim of this case is to raise awareness among dentists who treat children to consider the biological benefits of SDF in situations, such as the current contingency, as well as the benefits of mitigating the aesthetic effects thanks to the use of potassium iodide. CPD/Clinical Relevance: The use of silver diamine fluoride in a paediatric patient with early childhood caries is highlighted.
Neutral zone concept for implant placement in mandibular overdentures: a case report
Authors: AL-Oulabi Ayman , Johari Yanti , Husein Adam , Hassan Akram , Shaari Ramizu , Ariffin Azirrawani 
Published: June 2022

The objectives for complete denture fabrication are to provide comfort, function and aesthetics. The availability of implant-assisted overdentures has benefited complete denture wearers; however, the success of the prostheses can be further enhanced when the neutral zone concept is incorporated as a mean to locate the correct position of the implants, as well as the tooth arrangement for optimal outcome of the prescribed prostheses. CPD/Clinical Relevance: Use of the neutral zone concept to assist implant placement and teeth setting in a severely resorbed edentulous mandible is beneficial.
Nutritional potential of Semele sp. shellfish on estrogen levels and follicle stimulating hormone receptor genes in perimenopausal women
Authors: SJAFARAENAN SJAFARAENAN , UMAR MUHAMMAD RUSLAN , HERWIN HERWIN , ISLAMIYATI ANNA , TUWO MUSTIKA , ZULKIFLI ANDI 
Published: June 2022

Abstract. Sjafaraenan, Umar MR, Herwin, Islamiyati A, Tuwo M, Zulkifli A. 2022. Nutritional potential of Semelesp. shellfish on estrogen levels and follicle stimulating hormone receptor genes in perimenopausal women. Biodiversitas 23: 1196-1203. The Bonea villagecommunity that often consume shellfish usually have a high libido and experience menopause abovethe age of 50 years.Therefore, this study aims to determinethe nutritional potential of Semele sp.on the Follicle-Stimulating Hormone Receptor (FSH-R) gene in perimenopausal womenas well asthe levels of estrogen and polymorphisms.The experimental method with a one-group preandpost-test design was used by measuring 10 perimenopausal women respondents aged 40-45 before and after consuming Semele sp. 2 times a week for 2 months. Furthermore, blood samples were collected through the median cubital vein 2-3 mL before and after the treatment, The nutritional measurement of Semele sp. including protein was carried out using the Kjeldhal method, carbohydrates using the Anthrone method, and fat using the gravimetry. Also, the mineral content was determined using Atomic Absorption Spectrophotometry(AAS), while the estrogen levels were assessed with the ELISA technique. The FSH gene was examined using DNA extraction and amplification, electrophoresis, as well as Restriction Fragment Length Polymorphism (RFLP) with the Bsrl enzyme, while thet-test was conducted to examinethe effect of shellfish consumption. The results showed that the consumption of Semele sp.increased estrogen levels significantly, as the Asn680Ser, Asn680Asn, and Ser680Se genotypes were found in each respondent with the FSH-R gene. Based on the results,it was concluded that the regular consumption of Semele sp. increases estrogen levels and is applicable as analternative treatment to delay the incidence of menopause.
Cellulose degrading bacteria isolated from Palangkaraya, Central Kalimantan, Indonesia as peat fiber decomposer to accelerate peat soil compression
Authors: ZULAIKA ENNY , SOLIKHAH FARIHATUS , UTOMO MUHAMMAD ANDRY PRIO , ENDAH NOOR , ASSAVALAPSAKUL WANCHAI 
Published: June 2022

Abstract. Zulaika E, Solikhah F, Utomo MAP, Endah N, Assavalapsakul W. 2022. Cellulose degrading bacteria isolated from Palangkaraya, Central Kalimantan, Indonesia as peat fiber decomposer to accelerate peat soil compression. Biodiversitas 23: 1648-1654. Peat soil was established from incomplete weathered plants. It delays the process of soil compression. The main components of peat soil are fibers which made of cellulose and lignin that can be enzymatically degraded by cellulolignolytic bacteria. The objective of this study was to isolate indigenous lignocellulolytic bacteria from peat soil that perform peat fiber degradation to accelerate soil compression process. A bacteria screening test was carried out on carboxymethyl cellulose (CMC) media and bacteria identification using 16S rRNA gene markers. Peat fiber decomposition test based on biomass loss, decomposition results were visualized using scanning electron microscopy (SEM) and analyzed using Fourier Transform Infrared (FTIR). The results of screening and identification obtained 5 bacterial species, namely Pseudomonas taiwanensis U3 and Bacillus cereus D1, D3, U2, U4. After six weeks of incubation, P. taiwanensis U3 showed the highest cellulase activity than other isolates with 90% decomposition ability of peat fibers. The results of the decomposition of peat fibers with the addition of isolates visualized smaller pore sizes than control. Stretching occurs in the aromatic group of the C-O ester bond, the CºC structure of the alkyne group, and the O-H of the alcohol. All obtained bacteria isolates were still viable after six weeks of incubation with 104 CFU g-1 dry weight of fiber. The present investigation deals with isolating indigenous lignocellulolytic bacteria from peat soil that perform peat fiber degradation to accelerate the soil compression process. Our findings are also the first publication that reported P. taiwanensis cellulolytic potential.
New lower bound for the minimal number of edges of simple uniform hypergraph without the property Bk
Authors: Demidovich Yury A. 
Published: June 2022

Abstract
A hypergraph H = (V, E) has the property Bk
if there exists an assignment of two colors to V such that each edge contains at least k vertices of each color. A hypergraph is called simple if every two edges of it have at most one common vertex. We obtain a new lower bound for the minimal number of edges of n-uniform simple hypergraph without the property Bk
.
Tissue concentration of aldosterone in fetal adrenals of intrauterine death cases
Authors: Dwivedi Daisy , Chander Bal 
Published: June 2022

Abstract

Objectives
Fetal adrenals are one of the main organs responsible for maturation and survival. Extant literature is not clear about whether second trimester fetal adrenals are capable of synthesizing secreting aldosterone.

Methods
We have taken 20 adrenals from fetuses of intrauterine death cases. None of the fetuses had any external malformations and obstetric history was unremarkable. The organs were weighed and homogenized. The supernatant was used for aldosterone estimation by ELISA.

Results
We consistently detected aldosterone in all the cases including second trimester. However, we did not see any correlation between aldosterone concentration and gestational age. It is striking to note that there are wide variations in the tissue levels of aldosterone across different gestational ages and also same period.

Conclusions
Tissue aldosterone levels in second trimester can be possibly induced by stress preceding intrauterine deaths. It is possible that functional status of adrenal is different in intrauterine death cases as opposed to elective abortions in second trimester.
Difference schemes for second-order ordinary differential equations with corrector and predictor properties
Authors: Shaidurov Vladimir V. , Novikov Anton E. 
Published: June 2022

Abstract
A technique for constructing a sequence of difference schemes with the properties of a corrector and a predictor for integrating systems of the second-order ordinary differential equations is presented. The sequence of schemes begins with the explicit three-point Störmer method of the second order of approximation. Each subsequent scheme also implements the Störmer method corrected with additional terms calculated through the solution of the previous scheme. The stability of the resulting schemes and the increase in the order of convergence for the first of them are carefully substantiated. The results of calculations of the test problem are presented, confirming the increase in the order of accuracy of the constructed methods.
Die Herausbildung von Nationalstaaten und territoriale Religionsgeschichten: Analysen zur Schweiz und Österreich
Authors: Lehmann Karsten , Baumann Martin 
Published: June 2022

Zusammenfassung
Der Artikel argumentiert, dass die Fokussierung religionswissenschaftlicher Forschungen auf religiöse Gegenwartskulturen von einer kritischen Auseinandersetzung mit den Bedingungen von Nationalstaatlichkeit profitieren kann. Zwar setzten sich zahlreiche Studien mit den staatlichen und politischen Kontextbedingungen von Religionsgeschichte auseinander, doch fehlen dabei auffallend häufig Bezüge zu den aktuellen historischen und politikwissenschaftlichen Konzepten von Nation und Nationalstaat. In Anlehnung an die Historiker Florian Bieber, Christian Jansen und Henning Borggräfe definieren wir den Begriff der Nation als ein Narrativ, auf das sich Mitglieder einer Gemeinschaft geeinigt haben und das die Grundlage für den Prozess der Nationenbildung darstellt. Anhand von zwei Fallbeispielen zur Nationalstaatsbildung in der Schweiz und Österreich zeigen wir die unterschiedlichen Einflussdynamiken von herrschaftlicher Macht, Nationalstaatsbildung, transnationalen Dimensionen und Durchsetzung des als säkular konzipierten Rechts auf die Ausformung der Religionsgeschichten. Die vergleichende Analyse generalisiert die Befunde von Nationalstaat und Religionsgeschichte und benennt drei Impulse für die weitere Forschung.
Differences in the spectrum of steroidogenic enzyme inhibition between Osilodrostat and Metyrapone in ACTH-dependent Cushing syndrome patients
Authors: Bonnet-Serrano Fidéline , Poirier Jonathan , Vaczlavik Anna , Laguillier-Morizot Christelle , Blanchet Benoît , Baron Stéphanie , Guignat Laurence , Bessiene Laura , Bricaire Léopoldine , Groussin Lionel , Assié Guillaume , Guibourdenche Jean , Bertherat Jérôme 
Published: June 2022

Abstract

Introduction
Osilodrostat is a new 11β-hydroxylase inhibitor with a mode of action analogous to Metyrapone. The objective of this study was to compare steroidogenic profiles in patients treated with either Osilodrostat or Metyrapone for adrenocorticotrophic hormone (ACTH)-dependent Cushing's syndrome (CS).

Methods
Patients followed up at Cochin hospital Endocrinology department between March 2019 and December 2021 for an ACTH-dependent CS, controlled by either Osilodrostat or Metyrapone, were included. A serum profile of five steroids (cortisol, 11-deoxycortisol, 17-hydroxyprogesterone, androstenedione and testosterone) was determined using UPLC- tandem mass spectrometry (UPLC-MS/MS).

Results
Nineteen patients treated with Osilodrostat, eight patients treated with Metyrapone and six patients treated with consecutive Metyrapone then Osilodrostat were included. Hypocortisolism (basal cortisol &lt;100 nmol/L) was found in 48% of patients treated with Osilodrostat and 7% of patients treated with Metyrapone. 11-deoxycortisol and androstenedione levels were higher in patients treated with Metyrapone (80.9 (2.2–688.4) and 14.9 (2.5–54.3) nmol/L, respectively) than in patients treated with Osilodrostat (10.3 (0.5–71.9) and 4.0 (0.3–13.3) nmol/L) (P = 0.0009 and P = 0.0005). Testosterone level in women was also higher in Metyrapone group (3.3 (0.93–4.82) nmol/L vs 1.31(0.13–5.09) nmol/L, P = 0.0146). CYP11B1 activity (11-deoxycortisol/cortisol) was not significantly different between the two groups. CYP21A2 activity (17OHprogesterone/11-deoxycortisol) and CYP17A1 activity (17OHprogesterone/androstenedione) were significantly decreased in Osilodrostat group (P &lt; 0.0001).

Conclusion
In patients with ACTH-dependent CS, the use of CYP11B1 inhibitors in routine care suggests that Osilodrostat has a less specific effect on the inhibition of steroidogenic enzymes than Metyrapone. This might explain a smaller increase in 11-deoxycortisol and androgen levels in patients treated with Osilodrostat.
Rubén Darío en Mallorca, cara a cara con Dios
Authors: Argüello Lacayo José 
Published: June 2022

En su novela autobiográfica, El oro de Mallorca, Rubén Darío desnuda su alma y muestra sus más hondas inquietudes religiosas. El presente artículo examina las vivencias del poeta durante su última y decisiva visita a la isla de Mallorca a fines de 1913. Allí hace balance de su vida, mientras se debate interiormente entre su anhelo de Dios y las debilidades y dudas que le acosan.