bluuebunny/crossref_metadata_2025_split huggingface dataset:
The transcription factor c-Jun inhibits RBM39 to reprogram pre-mRNA splicing during genotoxic stress
Authors: Lemaitre Florence , Chakrama Fatima , O’Grady Tina , Peulen Olivier , Rademaker Gilles , Deward Adeline , Chabot Benoit , Piette Jacques , Colige Alain , Lambert Charles , Dequiedt Franck , Habraken Yvette 
Published: December 2022

Abstract
Genotoxic agents, that are used in cancer therapy, elicit the reprogramming of the transcriptome of cancer cells. These changes reflect the cellular response to stress and underlie some of the mechanisms leading to drug resistance. Here, we profiled genome-wide changes in pre-mRNA splicing induced by cisplatin in breast cancer cells. Among the set of cisplatin-induced alternative splicing events we focused on COASY, a gene encoding a mitochondrial enzyme involved in coenzyme A biosynthesis. Treatment with cisplatin induces the production of a short isoform of COASY lacking exons 4 and 5, whose depletion impedes mitochondrial function and decreases sensitivity to cisplatin. We identified RBM39 as a major effector of the cisplatin-induced effect on COASY splicing. RBM39 also controls a genome-wide set of alternative splicing events partially overlapping with the cisplatin-mediated ones. Unexpectedly, inactivation of RBM39 in response to cisplatin involves its interaction with the AP-1 family transcription factor c-Jun that prevents RBM39 binding to pre-mRNA. Our findings therefore uncover a novel cisplatin-induced interaction between a splicing regulator and a transcription factor that has a global impact on alternative splicing and contributes to drug resistance.
Distinct contribution of two cyclic electron transport pathways to P700 oxidation
Authors: Zhou Qi , Yamamoto Hiroshi , Shikanai Toshiharu 
Published: December 2022

Abstract
Cyclic electron transport (CET) around Photosystem I (PSI) acidifies the thylakoid lumen and downregulates electron transport at the cytochrome b6f complex. This photosynthetic control is essential for oxidizing special pair chlorophylls (P700) of PSI for PSI photoprotection. In addition, CET depending on the PROTON GRADIENT REGULATION 5 (PGR5) protein oxidizes P700 by moving a pool of electrons from the acceptor side of PSI to the plastoquinone pool. This model of the acceptor-side regulation was proposed on the basis of the phenotype of the Arabidopsis (Arabidopsis thaliana) pgr5-1 mutant expressing Chlamydomonas (Chlamydomonas reinhardtii) plastid terminal oxidase (CrPTOX2). In this study, we extended the research including the Arabidopsis chlororespiratory reduction 2-2 (crr2-2) mutant defective in another CET pathway depending on the chloroplast NADH dehydrogenase-like (NDH) complex. Although the introduction of CrPTOX2 did not complement the defect in the acceptor-side regulation by PGR5, the function of the NDH complex was complemented except for its reverse reaction during the induction of photosynthesis. We evaluated the impact of CrPTOX2 under fluctuating light intensity in the wild-type, pgr5-1 and crr2-2 backgrounds. In the high-light period, both PGR5- and NDH-dependent CET were involved in the induction of photosynthetic control, whereas PGR5-dependent CET preferentially contributed to the acceptor-side regulation. On the contrary, the NDH complex probably contributed to the acceptor-side regulation in the low-light period but not in the high-light period. We evaluated the sensitivity of PSI to fluctuating light and clarified that acceptor-side regulation was necessary for PSI photoprotection by oxidizing P700 under high light.
A novel estrogen receptor 1: sphingomyelin phosphodiesterase acid-like 3B pathway mediates rituximab response in myositis patients
Authors: Parkes Joanna E , Boehler Jessica F , Li Ning , Kendra Ryan M , O’Hanlon Terrance P , Hoffman Eric P , Peterson Jennifer M , Miller Frederick W , Rider Lisa G , Nagaraju Kanneboyina 
Published: December 2022

Abstract

Objectives
The B-cell depleting biologic, rituximab, is used to treat refractory autoimmune myositis. However, the beneficial effects of rituximab appear to outweigh the known contribution of B cells in myositis. We aimed to elucidate how myositis patients respond differently to rituximab and possible alternative mechanisms of action.

Methods
Here we have: (i) comprehensively investigated concurrent mRNA and microRNA expression in muscle biopsies taken at baseline and 16 weeks post treatment in 10 patients who were part of the rituximab in myositis (RIM) trial; and (ii) investigated the beneficial effect of rituximab on myositis muscle cells.

Results
Our analyses identified an increased number of changes in gene expression in biopsies from patients who had a clinical response to rituximab (n = 5) compared with non-responders (n = 5). The two groups had completely different changes in microRNA and mRNA expression following rituximab therapy, with the exception of one mRNA, BHMT2. Networks of mRNA and microRNA with opposite direction of expression changes highlighted ESR1 as upregulated in responders. We confirmed ESR1 upregulation upon rituximab treatment of immortalized myotubes and primary human dermatomyositis muscle cells in vitro, demonstrating a direct effect of rituximab on muscle cells. Notably, despite showing a response to rituximab, human dermatomyositis primary muscle cells did not express the rituximab target, CD20. However, these cells expressed a possible alternative target of rituximab, sphingomyelinase-like phosphodiesterase 3 b (SMPDL3B).

Conclusion
In addition to B-cell depletion, rituximab may be beneficial in myositis due to increased ESR1 signalling mediated by rituximab binding to SMPDL3B on skeletal muscle cells.
Tune in, Turn on: Religious Music and Spiritual Power in the History of Psychedelic Therapy
Authors: Lett Stephen , Dyck Erika 
Published: December 2022

Summary
Psychedelic-assisted therapy has attracted considerable clinical attention in the past decade for its ability to bring therapeutic benefits to patients in treatment-resistant categories. In contradistinction from other psychopharmaco-therapies, contemporary psychedelic therapists, like their predecessors, paid close attention to the ‘set and setting’, and argued that the mind-set of the subject and the conditions or environment of the session was as influential as the pharmacological reaction itself. In this paper, we examine how religious sounds and music were both incorporated into and strategically avoided in the early psychedelic therapeutic sessions in an effort to achieve spiritual epiphanies at peak experiences. Prominent contemporary practices, we conclude, recapitulate many of the practices of the past, relying, we argue, on aesthetic premises that could hinder the therapy’s broader applicability.
Severe dengue associated withStaphylococcus aureussepsis in pediatric patients: a case series
Authors: Sudeep K C , Kumar Surjeet , Randhawa Manjinder Singh , Angurana Suresh Kumar , Nallasamy Karthi , Bansal Arun , Muralidharan Jayashree 
Published: December 2022

AbstractBackgroundStaphylococcus aureus co-infection is seldom reported in children with severe dengue.MethodologyIn this retrospective study, we reported five children with severe dengue and S. aureus co-infection admitted to pediatric intensive care unit (PICU) during July–December 2021.ResultsAll children had prolonged fever, persistence of bilateral pleural effusion beyond the critical phase, thrombocytopenia and raised inflammatory markers [C-reactive protein (CRP) and procalcitonin]. S. aureus was isolated from pleural fluid (n = 2, 40%), blood (n = 2, 40%) and endotracheal aspirate (n = 1, 20%). Four children (80%) grew methicillin-sensitive S. aureus, while 1 (20%) had methicillin-resistant S. aureus. Two children (40%) had septic thromboemboli in skin, and 1 (20%) had limb cellulitis. One child required anterior thoracotomy, pericardiectomy and bilateral pleural decortication, while all other children required intercostal chest tube drainage. All children required prolonged targeted antibiotics, invasive mechanical ventilation and had prolong stay in PICU and all of them survived.ConclusionIn children with severe dengue, persistence of fever, persistence of pleural effusion beyond critical phase and raised CRP and procalcitonin should raise suspicion of bacterial/S. aureus co-infection.
Long-Term Auditory and Speech Outcomes of Cochlear Implantation in Children With Cochlear Nerve Aplasia
Authors: Chao Xiuhua , Luo Jianfen , Wang Ruijie , Hu Fangxia , Wang Haibo , Fan Zhaomin , Xu Lei 
Published: December 2022

Objectives:
In this study, we aimed to (1) review the long-term outcomes of cochlear implantation in children with cochlear nerve aplasia and (2) compare the development of their auditory and speech abilities to children with normal-sized cochlear nerves.

Design:
This is a retrospective case-control study. Patients who underwent unilateral cochlear implant (CI) surgery in a tertiary referral center from September 2012 to December 2018 were reviewed. The study group included 55 children with cochlear nerve aplasia diagnosed using preoperative images. The control group included 35 children with normal-sized cochlear nerves. The control group did not differ from the study group in terms of age at implantation, pre-implantation auditory and speech abilities, or the electrode array type. Cochlear implantation outcomes were assessed using a test battery, including the Categories of Auditory Performance (CAP) score, the Speech Intelligibility Rating (SIR) score, behavioral audiometry, and closed- or open-set speech recognition tests. The development of auditory and speech abilities was compared between the two groups using Generalized Linear Mixed-effect Models.

Results:
The mean duration of CI usage was 4.5 years (SD = 1.5, range = 2.0 to 9.5) in the study group. The CAP scores, SIR scores, and aided hearing thresholds improved significantly post-implantation in the study group, but were significantly poorer than those in the control group. Generalized Linear Mixed-effect Models showed that the development of CAP and SIR scores was significantly slower in the study group than in the control group. Overall, 27 (49%) children with cochlear nerve aplasia had some degree of open-set speech perception skills, but the monosyllabic and bisyllabic word recognition rates were significantly lower than those in the control group.

Conclusion:
For children with cochlear nerve aplasia, auditory perception and speech intelligibility continued to improve in the long-term follow-up, but this progress was significantly slower than in children with normal-sized cochlear nerves. Most children with cochlear nerve aplasia could obtain the ability of common phrase perception and understanding simple spoken language with consistent CI usage and auditory rehabilitation.
Inter-Subject Variability in the Dependence of Medial-Olivocochlear Reflex Strength on Noise Bandwidth
Authors: Lee Donguk , Lewis James D. 
Published: December 2022

Objectives:
The objective of the study was to quantify inter-subject variability in the dependence of the medial-olivocochlear reflex (MOCR) on noise bandwidth. Of specific interest was whether inter-subject variability in MOCR dependence on bandwidth explained variability in the MOCR response elicited by wideband noise.

Design:
Thirty-two young adults with normal hearing participated in the study. Click-evoked otoacoustic emissions were measured in the ipsilateral ear with and without noise presented in the contralateral ear. Presentation of contralateral noise served to activate the MOCR. The MOCR was activated using five different noise stimuli with bandwidths ranging from 1- to 5-octaves wide (center frequency of 2 kHz; bandwidth incremented in 1-octave steps). Noise spectral levels (19.6 dB SPL/Hz) were held constant across all bandwidths. MOCR metrics included the normalized-percent change in the otoacoustic emission (OAE), the MOCR-induced OAE magnitude shift, and the MOCR-induced OAE phase shift. Linear mixed-effect models were fit to model the dependence of MOCR-induced OAE magnitude and phase changes on noise bandwidth. The use of a mixed-effect modeling approach allowed for the estimation of subject-specific model parameters that capture on- and off-frequency contributions to the MOCR effects. Regression analysis was performed to evaluate the predictive capacity of subject-specific model parameters on the MOCR response elicited by wideband noise.

Results:
All OAE-based MOCR metrics increased as the noise bandwidth increased from 1- to 5-octaves wide. The dependence of MOCR-induced OAE magnitude and phase shifts on activator bandwidth was well approximated using a linear model with intercept and slope terms. On average, MOCR-induced magnitude and phase shifts increased at a rate of 0.3 dB/octave and 0.01 cycles/octave, respectively, as bandwidth extended beyond the predicted region of OAE generation. A statistically significant random effect of subject was found for both the intercept and slope parameter of each model. Subject-specific slope estimates were statistically significant predictors of a repeated measure of the wideband MOCR response. A higher slope was predictive of larger wideband MOCR effects.

Conclusions:
MOCR-induced changes to the OAE are greatest when the MOCR is elicited using wideband noise. Variability in the process of spectral integration within the MOCR pathway appears to explain, in part, inter-subject variability in OAE-based estimates of the MOCR response elicited by wideband noise.
Family Presence on Rounds in Adult Critical Care: A Scoping Review
Authors: Calderone Alexander , Debay Vanessa , Goldfarb Michael J. 
Published: December 2022

OBJECTIVES:
Family presence on rounds involves allowing family members to participate in daily healthcare team rounds and is recommended by critical care professional societies. Yet, family presence on rounds is not performed in many institutions. There is a need to synthesize the current evidence base for this practice to inform healthcare providers of the potential benefits and challenges of this approach. The main objective of this study was to explore the impact of family presence on adult ICU rounds on family and healthcare providers.

DATA SOURCES:
Ovid Medline, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Cochrane Library, and PubMed databases were last searched on January 28, 2022. Studies published during the COVID-19 pandemic were included.

STUDY SELECTION:
Studies involving family presence during rounds that included family or healthcare provider perspectives or outcomes were selected. There were no limitations on study design.

DATA EXTRACTION:
Qualitative and quantitative family and provider perspectives, barriers and challenges to family presence, and study outcomes were extracted from studies. The JBI Manual for Evidence Synthesis published guidelines were followed.

DATA SYNTHESIS:
There were 16 studies included. Family reported family presence on rounds as a means of information transfer and an opportunity to ask care-related questions. Family presence on rounds was associated with increased family satisfaction with care, physician comfort, and improved physician-family relationship. Healthcare providers reported a positive perception of family presence on rounds but were concerned about patient confidentiality and perceived efficacy of rounds. Family presence was found to increase rounding time and was felt to negatively impact teaching and opportunities for academic discussions.

CONCLUSIONS:
Family presence on rounds has potential advantages for family and healthcare providers, but important challenges exist. Further studies are needed to understand how to best implement family presence on adult ICU rounds.
Echocardiographic Evidence of Cardiac Atrophy in the Critically Ill
Authors: Kumaresan Abirami , Pannu Ameeka , Mueller Ariel , De Lima Andres , Naseem Heba , O’Connor Sean P. , Valencia Juan , Garcia Daniela , Gosling Andre Fiche , Talmor Daniel , Shaefi Shahzad 
Published: December 2022

OBJECTIVES:
The purpose of this explorative study is to determine if critically ill patients experience cardiac atrophy that can be quantified as a loss of left ventricular mass (LVM) and thus detected by echocardiography.

DESIGN:
Retrospective single-center cohort study.

SETTING:
Patients admitted to a tertiary medical center in Boston, MA.

PATIENTS:
Adult critically ill patients with ICU length of stay greater than or equal to 5 days.

INTERVENTIONS:
None.

MEASUREMENTS AND MAIN RESULTS:
We conducted a retrospective cohort study of 68 patients, of which 42 were included in the final analysis (mean age 60.9 ± 19.2 yr; 47.6% male). The median length of ICU stay was 11.3 days (interquartile range, 6.8–20.1 d). A decrease in mean LVM over the course of admission for critical illness was observed (median 189.11 g [162.82–240.20 g] vs 176.69 g [142.37–226.26 g]; p = 0.01). After adjusting for sex, age, fluid balance, ICU type, dietary orders, time between echocardiograms, and vasopressor use, this decrease in LVM remained consistent (mean difference, –21.30 g; 95% CI, –41.85 to –0.74; p = 0.04). Relative wall thickness (RWT) did not change during admission.

CONCLUSIONS:
These data reveal that a loss of LVM is present in patients over their ICU stay without a corresponding change in RWT, consistent with cardiac atrophy. Future prospective studies are needed to confirm these findings and identify possible sequelae of this finding.
Correlates of Breast Cancer Screening in Arab Muslim Women Living in the United States
Authors: Mataoui Fatma Zohra 
Published: December 2022

Background:
Breast cancer is the most diagnosed cancer in women, with mammography being the most efficient at detecting early-stage breast cancer. Arab Muslim women living in the United States are an understudied minority group with cultural differences that may influence their utilization of mammography.

Objective:
Guided by the Neuman Systems Model, this study aimed to determine correlates of using mammography, including acculturation, modesty, and breast cancer knowledge, of Arab Muslim women living in New England aged 40 years and older.

Interventions/Methods:
A correlational study design was used with 118 Arab Muslim women with a survey that included sociodemographic data, acculturation, Islamic modesty, and breast cancer knowledge scales.

Results:
The screening rate for women aged 50 years and older was statistically significant in comparison to the Center for Disease Control report (2018) (Z = −2.15, P < .01). Multivariate analysis revealed that modesty (odds ratio = 0.90; confidence interval [0.81-0.99]; P = 0.04), decreased length of stay in the United States (r = 0.34; P < .01), and the use of the Arabic language in the survey (r = −0.20; P < .05) were negatively associated with mammography use.

Conclusions:
Educational and cultural factors may influence use of mammography screening by Arab Muslim women living in the United States.

Implications for Practice:
The study findings underscore the need for a better understanding of modesty by health care providers to enhance use of mammography by Arab Muslim women.

What is Foundational:
Cultural, educational, and religious variables may be drivers of decreased mammography use in minority women. Barriers to breast cancer detection can be overcome by improving sensitivity to such variables.
Establishment of a Cancer Acute Care Clinic Within an Infusion Center
Authors: Alfaro Emely , Quinn Marisa , Baltic Tammy , Acebedo Christopher , Israni Manisha , Miaskowski Christine 
Published: December 2022

Context:
With the release of an outcome-based quality measure from the Centers for Medicare and Medicaid Services that aimed to reduce emergency department (ED) and hospital admissions for patients receiving outpatient chemotherapy, new models of care needed to be implemented.

Clinical Setting:
This case study describes development and implementation of a cancer acute care clinic (CACC) within an extremely busy infusion center located in a National Cancer Institute-designated Comprehensive Cancer Center. Modifications that were necessary in the context of COVID-19 pandemic are described.

Process:
The development of the CACC involved a transdisciplinary team who determined CACC’s purpose; established patient admission criteria; created a nursing leadership and staffing plan; developed initial operating procedures; and made necessary adjustments to provide care during the COVID-19 pandemic. Clinical pathways were developed to guide care. Informal patient and staff surveys were done to evaluate satisfaction with CACC’s services.

Outcomes:
Of the initial 142 visits, 75.4% of patients were discharged home, and 12.6% and 12.0% were admitted to the ED or hospital, respectively. Both patients and staff reported high levels of satisfaction with CACC services.

Conclusion:
While COVID-19 disrupted plans for an evaluation of reductions in ED visits, because of high levels of staff and patient satisfaction, plans are underway to expand CACC services and conduct more formal outcome evaluations.

Implications for Practice:
Description of the processes involved in the development and implementation of the CACC may serve as a model for other organization on how to provide acute care to oncology patients.
Clinical Predictors of Adherence to Exercise Training Among Individuals With Heart Failure
Authors: Collins Katherine A. , Reeves Gordon R. , Miller Nancy Houston , Whellan David J. , O'Connor Christopher M. , Marcus Bess H. , Kitzman Dalane W. , Kraus William E. 
Published: December 2022

Purpose:
Suboptimal adherence is a major limitation to achieving the benefits of exercise interventions, and our ability to predict and improve adherence is limited. The purpose of this analysis was to identify baseline clinical and demographic characteristics predicting exercise training adherence in the HF-ACTION study cohort.

Methods:
Adherence to exercise training, defined by the total duration of exercise performed (min/wk), was evaluated in 1159 participants randomized to the HF-ACTION exercise intervention. More than 50 clinical, demographic, and exercise testing variables were considered in developing a model of the min/wk end point for 1-3 mo (supervised training) and 10-12 mo (home-based training).

Results:
In the multivariable model for 1-3 mo, younger age, lower income, more severe mitral regurgitation, shorter 6-min walk test distance, lower exercise capacity, and Black or African American race were associated with poorer exercise intervention adherence. No variable accounted for >2% of the variance and the adjusted R
2 for the final model was 0.14. Prediction of adherence was similarly limited for 10-12 mo.

Conclusions:
Clinical and demographic variables available at the initiation of exercise training provide very limited information for identifying patients with heart failure who are at risk for poor adherence to exercise interventions.