bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of MTT Assay for Proliferation of U937 Cell Line and its Inter-rater Reliability - Best Taken with a Grain of Salt
Authors: Wakode S. , Trushna T. , Wakode N. , Thakare A. , Bharshankar R. , Manjunatha S. 
Published: November 2022

Background Biomedical research frequently employs immortal human cell lines to study various physiological and pathological topics of interest. An important problem that is often ignored is the question of reliability of observations made while using a particular assay on cell lines, especially MTT Assay. Recent studies have questioned its reliability by highlighting the diverse intracellular environmental factors that affect accuracy of MTT assay results.
Objective To assess the inter-rater reliability of MTT assay while observing the differential effect of addition of fetal bovine serum to the culture medium on proliferation of U937 cell line using Intraclass correlation coefficient.
Method U937 cell suspension prepared by the tutor to maintain uniformity of immortalized cell line was used. Four trainee investigators who participated in the study underwent rigorous training in pipetting and plating methods for four consecutive days prior to start of the experiment. Each trainee investigator followed the same protocol and all procedures were conducted simultaneously. At the end of the experiment, inter observer reliability was calculated.
Result Interrater reliability calculated by using intra class correlation coefficient with two way mixed effect model was found to be poor (p = 0.173). For growth with 10% fetal bovine serum, inter-rater reliability was 0.258 for each item and 0.58 for the average of the triplicates, whereas for 0% fetal bovine serum it was 0.374 for each item and 0.70 for average of the values (p=0.104).
Conclusion Though MTT assay is considered the gold standard test for assessment of cell proliferation and viability, the inter-rater reliability of this assay might be poor and needs further investigation.
Peduli Covid 19 Melalui Pembagian Masker Diwilayah Kota Mataram
Authors: Fitriani Eka , Mahsup Mahsup 
Published: November 2022

Abstract: The purpose of this devotional activity is to know the benefits of using masks in preventing the transmission of covid 19 in mataram city area. The devotional approach used is a qualitative approach. While the method of devotion with descriptive methods. Community service activities are conducted in Mataram City and activities are carried out for one day. The source of data in the service activities is the community in the mataram city area. The data collected in this devotional activity is field record data. The result of community service activities that with the distribution of masks is very useful in anticipating the transmission of covid 19 contained in the saliva splash of a sick person when he sneezes, coughs or even while talking.Abstrak. Tujuan kegiatan pengabdian ini adalah untuk mengetahui manfaat menggunakan masker dalam mencegah penularan covid 19 diwilayah kota Mataram. Pendekatan Pengabdian yang digunakan adalah pendekatan kualitatif. Sedangkan metode pengabdianya dengan metode deskriptif. Kegiatan pengabdian kepada masyarakat dilakukan di Kota Mataram dan kegiatan dilakukan selama satu hari. Sumber data dalam kegiatan pengabdian adalah masyarakat yang ada diwilayah Kota Mataram. Data yang dikumpulkan dalam kegiatan pengabdian ini berupa data catatan lapangan. Hasil kegiatan pengabdian kepada masyarakat bahwa dengan pembagian masker sangat bermanfaat dalam mengantisipasi penularan covid 19 yang terdapat pada percikan air liur orang yang sakit ketika dia bersin, batuk atau bahkan saat berbicara.
Workshop Pengelolaan Jurnal dalam Lingkup Prodi Pendidikan Matematika
Authors: Permatasari Dian 
Published: November 2022

Abstract: Community service is an effort to share knowledge about journal management for journal managers so that they can manage journals properly and correctly. This service is carried out by holding a workshop followed by a journal review. used are workshops and journal surgery. The workshop was held twice and continued with journal review to find out the shortcomings of each journal. The methods used are workshops and journal surgery. The results obtained during the journal management workshop were that the participants knew the management of the journal from a managerial perspective as well as from an article editing perspective. It is hoped that after obtaining the journal management workshop, participants can prepare their journals for national and international accreditation. Improving the quality of scientific journal management is one of the basic components that greatly affects the quality of published journals. Journal managers who are not equipped with good journal management skills will certainly not be able to carry out a good journal management process. This Community Service (PkM) activity carried out in the Mathematics Education Study Program is very useful for journal managers. Especially for managers who have planned to submit their journals to register with the National Journal Accreditation (Arjuna) BRIN. This PkM is also an opportunity for journal managers to correct deficiencies in their respective journals. After understanding what are the shortcomings in the journals that are managed, it will be easier to fix them. Abstrak: Pengabdian kepada masyarakat merupakan salah satu upaya untuk sharing pengetahuan mengenai pengelolaan jurnal bagi para pengelola jurnal agar dapat mengelola jurnal dengan baik dan benar. Pengabdian ini dilakukan dengan mengadakan workshop dilanjutkan dengan bedah jurnal. yang digunakan adalah workshop dan bedah jurnal. Workshop dilaksanakan sebanyak dua kali dan dilanjutkan dengan bedah jurnal untuk mengetahui kekurangan dari masing-masing jurnal. Hasil yang diperoleh selama workshop pengelolaan jurnal adalah peserta mengetahui pengelolaan jurnal dari segi managerial maupun dari segi penyuntingan artikel. Kegiatan Pengabdian kepada Masyarakat (PkM) yang dilaksanakan di Prodi Pendidikan Matematika ini, sangat bermanfaat sekali bagi para pengelola jurnal. Terutama bagi pengelola yang sudah merencanakan untuk mengajukan jurnalnya mendaftar pada Akreditasi Jurnal Nasional (Arjuna) BRIN. PkM ini juga menjadi ajang bagi para pengelola jurnal untuk memperbaiki kekurangan-kekurangan pada jurnal mereka masing-masing. Setelah memahami apa yang menjadi kekurangan pada jurnal yang dikelola, maka akan lebih mudah untuk memperbaikinya.
The Impact of Social Class and Ability Group on Students Academic Achievement: a Case Study of Selected Primary Schools
Authors: Salmah Siti , Nisak Khoirun , Syafiani Angela , Bulan Linda Sari 
Published: November 2022

Ability group system (AGS) can be understood as grouping students according to their academic abilities. There are pros and cons of the ability group implementation in schools. However, this system is still practised in modern national educational systems. The present study explored the impact of students’ socioeconomic status (SES) and the AGS implementation on selected primary students’ academic achievement. The research utilised the qualitative method and followed a case study design confined to a selected primary school that implemented the ability group system in their teaching and learning process. A total of 12 informants were selected using the purposive sampling technique. The sample consisted of four teachers with experience adopting ability groups in the teaching and learning process and eight parents who have children in different ability level classes. Data was obtained using semi-structured interviews, non-participant classroom observation, and document analysis. The findings demonstrate that SES background and AGS could impact students’ academic performance. Therefore, this study suggested that the improving of group ability implementation in schools is crucial, such as teaching method and strategies that teacher use in different level of group ability should be improved. Also schools should provide a sufficient training for teacher to implement group ability system.
Using Anime to Enhance Learning Motivation and Japanese Listening Skills
Authors: Wisniawati Wisniawati , Rasiban Linna Meilia , Dewanty Via Luviana 
Published: November 2022

Teaching and learning activities Japanese listening skills need the motivation to support learning. However, learning motivation sometimes fluctuates, and the use of learning methods and media is not precise. This study aims to analyze the level of motivation and listening ability of students after using anime learning media. This study used a qualitative method with a note-taking technique and the sample used in this study are 16 students Departement of Japanese language in Bandung State West Java. The results of data showed that the use of anime in listening comprehension learning was able to increase learning motivation and listening skills. This is evidenced by the activities carried out by students when learning takes place and the value of assignments obtained by students has increased.
Dampak Pandemi Covid-19 Terhadap Pengelolaan Kas Bendahara Penerimaan Pemerintah Daerah
Authors: Akhmadi Muhammad Heru , Rachmalia Arda Octaviola 
Published: November 2022

: Cash is an important instrument for a company. The company’s financial performance can be judged by how the company's cash is managed. During the Covid-19 pandemic in 2020, the government decided to close tourist attractions. This study aims to find out how the performance of cash management during the Covid-19 pandemic was carried out by the Revenue Treasurer at the Pati Regency Youth Sports and Tourism Office. This study uses a descriptive qualitative method to analyze the cash management process in the Revenue Treasurer such as planning, recording, to depositing cash into local government accounts. Data were collected by interview and literature study. The results showed that in general, the Revenue Treasurer has managed cash well, starting from cash planning, recording, to depositing cash. However, the Covid-19 pandemic in 2020 has caused the number of regional revenues from the tourism sector to decline significantly. Some of the obstacles of the Revenue Treasurer in carrying out the cash receipt are the existence of multiple positions, weak communication, and the limited number of collecting officers at tourist attractions. Furthermore, the Revenue Treasurer keeps consistently recording as well as the period of cash is received. the Revenue Treasurer deposited the cash into the regional treasury account on the same day as cash is received. There were evidence of cash deposit in the form of a Deposit Certificate which has been validated by a partner bank appointed by the regional government.
Performance of LAD-LASSO and WLAD-LASSO on High Dimensional Regression in Handling Data Containing Outliers
Authors: Cahya Septa Dwi , Sartono Bagus , Indahwati Indahwati , Purnaningrum Evita 
Published: November 2022

In several research areas, it is common to have a dataset with more explanatory variables than the number of observations, called high-dimensional data. This condition can lead to multicollinearity problem. The least absolute shrinkage and selection operator (LASSO) solves the problem by shrinking the estimated coefficient to zero so that it can simultaneously carry on the variable selection and the parameter estimation. But LASSO performs poorly when the data contains some outliers in the response or explanatory variables. Robust methods have addressed this problem based on the least-absolute-deviation approach, such as LAD-LASSO and WLAD-LASSO. This current research aims to evaluate the performance of the LAD-LASSO and WLAD-LASSO methods on high-dimensional and low-dimensional data containing outliers. To evaluate the performance of these methods, the simulation study was conducted. The simulation study used three scenarios (without outliers, outliers on the response variable (5%, 10%, 15%), outliers both on the response and explanatory variables (5%, 10%, 15%)). We also used the Minimum Regularized Covariance Determinant (MRCD) estimator in calculating the weights on the WLAD-LASSO. The best method from this simulation then will be applied to sembung leaf extract data to identify antioxidant marker compounds in sembung leaf extract. The simulation results show that LAD-LASSO tends to be very tight in selecting, while LASSO tends to be too loose. Meanwhile, WLAD-LASSO is in the middle of those two techniques and performs the best in identifying the important variables correctly. Even the existence of weights cause WLAD-LASSO more robust against the presence of outliers in the response and explanatory variables compared to LAD-LASSO. Furthermore, performance of these methods on high-dimensional data decrease compared to low-dimensional data. The performance of these methods also tends to decrease when the rate of outlier increases. The WLAD-LASSO was then implemented in actual data to find the compound of antioxidant markers in the sembung leaf extract. The compounds/formulas obtained are Umbelliferone, 12-Hydroxyjasmonic Acid, C22H14N8O2, and Acetyleugenol (with a prediction error is 0.133050). These compounds/formulas can be developed as natural antioxidants and have the potential to be developed as medicinal ingredients.
Etnobotani Tumbuhan Obat Tradisional di Suku Colol Kabupaten Manggarai Timur
Authors: Samsi Andi Nur , Gustina Gustina , Susandri Karolina 
Published: November 2022

Abstract: This research is research on Ethnobotany of Traditional Medicinal Plants in the Colol Tribe, Ulu Wae Village, East Manggarai Regency. The purpose of this study was to find out what plants are used by the community in the Colol Tribe, Ulu Wae Village, the use and processing of these plants for traditional medicine, and what parts of the plants are used in treatment. The method used in this study is a structured interview, namely an interview that has been planned by referring to a list of questions that have been prepared. The data from the research results were analyzed descriptively and qualitatively according to the research objectives which would later be presented in the form of tables, pictures, or photos. The plants commonly used by people in the Colol tribe are turmeric (Curcuma domestica), temulawak (Curcuma zanthorrhiza), ginger (Zingiber officinale), betel (Piper betle), and jatropha (Jatropha curcas). The processing of medicinal plants carried out by the community in Ulu Wae Village is grated, pounded, cut, sliced, boiled, burned, chewed, drunk, and applied to the sick part. Diseases that can be cured include rheumatism, high blood pressure, cough, vaginal discharge, burns, toothache, fever, shortness of breath, liver, backache, dysentery, malaria, urinary stones, kidney stones, gout, and headaches.Abstrak: Peran tumbuhan yaitu dapat menjadi obat yang mudah ditemukan oleh masyarakat. Penelitian ini penting untuk mengetahui tumbuhan apa saja yang dimanfaatkan oleh masyarakat di Suku Colol Desa Ulu Wae, pemanfaatan, pengolahan tumbuhan tersebut untuk pengobatan tradisional dan bagian – bagian tumbuhan apa yang digunakan dalam pengobatan. Metode yang digunakan pada penelitian ini adalah wawancara testruktur (structured interview) yaitu wawancara yang telah terencana dengan berpedoman pada daftar pertanyaan yang telah dipersiapkan. Hasil penelitian dianalisis secara deskriptif kualitatif. Data disajikan dalam bentuk tabel, gambar, dan foto. Tumbuhan yang biasa digunakan oleh masyarakat di Suku Colol adalah kunyit (Curcuma domestica), temulawak (Curcuma zanthorrhiza), jahe (Zingiber officinalec), sirih (Piper betle), dan jarak pagar (Jatropha curcas). Pengolahan tanaman obat yang dilakukan oleh masyarakat di Desa Ulu Wae yaitu diparut, ditumbuk, dipotong, diiris, direbus, dibakar, dikunyah, diminum, dan dioleskan pada bagian yang sakit. Penyakit yang dapat disembuhkan antara lain rematik, darah tinggi, batuk, keputihan, luka bakar, sakit gigi, demam, sesak nafas, lever, sakit pinggang, disentri, malaria, kencing batu, batu ginjal, asam urat,dan sakit kepala.
The main stages in the formation and development of the concept of Pan-Africanism: History and modernity
Authors: Kassae N.V. Mikael 
Published: November 2022

The article is devoted to the analysis of the concept of Pan-Africanism. The authors propose to trace the evolution of the concept by dividing the stages of its formation into three periods: American, European and directly African. This is primarily due to the historical reasons for the formation of Pan-Africanism as a concept: from the struggle of the African-American population for their rights to the use of the concept as an ideological basis for a number of political regimes in Africa. Each stage gave the concept new features.
The American stage gave birth to the foundations of Pan-Africanism, putting on the agenda the idea of the struggle of individual ethnic groups for the rights and freedoms adopted in the United States. The European stage significantly expanded the geography and scope of the problems, as well as touched upon colonial issues.
The African stage was marked by an attempt to bring the concept of pan-Africanism into practice. This division has also given rise to a research question about the cause-and-effect relationships between the theoretical postulates of the concept and practical application at the present stage. It was during this period that it became clear how Pan-Africanism contributed to the fight against the oppression of various ethnic groups. As conclusions, answers are offered to the question whether the concept of Pan-Africanism can become a practical embodiment of the struggle of Africans for independence from Western countries.
Assessment of squalene effect on antioxidant enzymes and free radicals in patients with type 2 diabetes mellitus
Authors: Martirosyan Danik , Ashoori Mohammad Reza , Serani Anna , Zhang Kevin , Mirmiranpour Hossein 
Published: November 2022

Background: Diabetes mellitus as a metabolic disease can have serious consequences. Due to their chemical properties,bioactive compounds can play a role in diabetes management. Squalene is a natural oil and bioactive compound. The anti-inflammatory and antioxidant effects of squalene have been discussed in recent studies. Squalene plays a role in controlling diabetes by maintaining the oxidant/antioxidant balance.Objective: The main purpose of this study was to evaluate the antioxidant effect of different doses of squalene, on different days, on the levels of some oxidative indices and the activity of antioxidant enzymes in groups of people with type 2 diabetes and compare them with each other and healthy people.Methods: 150 individuals were recruited in this study. These individuals were separated into five groups. Group one contained 30 individuals, representing the healthy control group. Groups 2, 3, 4 and 5 included subjects with type 2 diabetes. Each of the subjects in groups 3, 4 and 5 received squalene in doses of 200, 400 and 600 mg as an oral capsule (liquid filled oral), respectively for 84 days. Subjects in Group 2 did not receive squalene. Catalase, superoxide dismutase, glutathione peroxidase (as antioxidant indicators) activities and the levels of hydrogen peroxide, nitric oxide and reactive oxygen species (as oxidant indicators) were assayed.Results: In 84 days, a statistically significant difference (P value < 0.05) was observed in all the diabetic groups compared to the healthy group. In the comparison between groups receiving squalene with each other, there was a significant increase (P value < 0.05) in catalase and superoxide dismutase activity, depending on squalene dose and time. There was not a statistically significant (P value > 0.05) increase in glutathione peroxidase activity. Statistically significant changes in oxidative indices were not dose-dependent or time-dependent.Conclusion: Based on the findings of this study, a dose of 600 mg of squalene in 84 days is effective in increasing catalase and superoxide dismutase activity and reducing hydrogen peroxide levels. Squalene can play an important role in controlling and reducing the consequences of diabetes caused by changes in the oxidant/antioxidant balance.Keywords: squalene, type 2 diabetes, T2D, antioxidant enzymes, bioactive compounds, free radicals
Identification of prognostic biomarkers among ICAMs in the breast cancer microenvironment
Authors: Chen Heyan , Pu Shengyu , Mei Nan , Liu Xiaoxu , He Jianjun , Zhang Huimin 
Published: November 2022

BACKGROUND: Intercellular adhesion molecules (ICAMs) in the tumor microenvironment are closely related to immunity and affect the prognosis of cancer patients. OBJECTIVE: The aim of our study is to explore the correlation between ICAM expression, mutation, methylation and immunity and their prognostic value in breast cancer (BC) is not clear. METHODS: Online databases and tools such as UALCAN, COSMIC, cBioPortal, MethSurv, PrognoScan, Kaplan-Meier Plotter, GSCA and TIMER were utilized in this study. RESULTS: We found that the mRNA and protein expression levels of ICAM1 were upregulated in triple-negative breast cancer (TNBC) compared with normal tissues, and TNBC patients with high expression of ICAM1 had better overall survival (OS) and recurrence-free survival (RFS). The main types of ICAM1 gene variants were missense mutation and amplification, and ICAM1 showed a lower level of methylation in TNBC cancer tissues than in normal tissues, which was contrary to the high expression levels of ICAM1 mRNA and protein. Next, the function of ICAM1 was mainly related to the activation of apoptosis, epithelial-mesenchymal transition (EMT) and inhibition of the androgen receptor (AR) and estrogen receptor (ER) pathways. Meanwhile, functional pathway enrichment results showed that ICAM1 was also involved in the immune regulation process of BC. Furthermore, the expression of ICAM1 was positively associated with 6 types of tumor-infiltrating immune cells (CD8+ T cells, CD4+ T cells, B cells, neutrophils, macrophages and dendritic cells) and was also positively related to the expression of programmed cell death-1 (PD-1), programmed cell death-ligand 1 (PD-L1) and cytotoxic T lymphocyte-associated antigen-4 (CTLA4). CONCLUSIONS: Our research indicated that ICAM1 was likely to be a potential therapeutic target in TNBC.
BANK INTEREST HALAL: DISTINCTION INTERPRETATION OF CONTEMPORARY ULAMA THOUGHT UMER CAPHRA AND MUHAMMAD SAYYID THANTHAWI
Authors: Huda Miftahul , Nabila Fikriyyatun , Fajriati Isma Fathya , Rahmah Listya , Mokan Zaimudin Al-Mahdi 
Published: November 2022

Life is controlled by two great forces, namely economy, and religion. All things based on Sharia law are clearly about halal and haram. Bank interest is something that is no longer common among the public, with the law and practice still being debated by some scholars. Therefore, this study aims to answer public confusion about the law of halal interest according to Umer Caphra and Muhammad Sayyid Thanthawi and the reasons for making legal decisions regarding bank interest. The research is in the form of a qualitative method with a literature study using several references from books and journals in concluding the views of the two figures. The primary data used is from the book Prohibition of Interest: Does it make sense? The work of Umer Chapra. While secondary data comes from various books and journals related to bank interest. The results of this study indicate that the two scholars have different opinions regarding bank interest. Umer Chapra argues that the practice of bank interest is prohibited in Islam because usury interest can harm the poor. Meanwhile, Muhammad Sayyid Thanthawi argues that bank interest is not part of usury because bank interest is not part of faith and worship.