bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Addition of Mifamurtide To Adjuvant Chemotherapy For Early Osteosarcoma: A Retrospective Analysis From Greece.
Authors: Kokkali Stefania , Kotsantis Ioannis , Magou Elpida , Sophia Talagani , Kormas Theodoros , Diakoumis Giakoumis , Spathas Nikolaos , Psyrri Amanda , Ardavanis Alexandros 
Published: February 2022

Abstract
Background: Current treatment recommendations for high grade non-metastatic osteosarcoma include perioperative chemotherapy and surgery. Despite this intensive protocol, approximately 40% of patients will relapse. The addition of mifamurtide to adjuvant cytotoxic chemotherapy was associated with a significant improvement in 6-year overall survival (OS) in young patients with resectable osteosarcoma, leading to its approval in Europe and other countries. Very limited real-world data are reported on its use.Methods: We retrospectively evaluated data from osteosarcoma patients who received mifamurtide in the adjuvant setting. Data were obtained from medical records in 2 high-volume bone sarcoma centers. The aim of this study was to collect real-world data on mifamurtide safety and efficacy in Greece. Results: We identified 15 patients with completely resected osteosarcoma who received mifamurtide from September 2015 to January 2020. Median age at diagnosis was 24 years old (16-76). Osteosarcoma arose in the lower extremities (n=12), in the upper extremities (n=2) or in the ilium (n=1). The majority of patients (n=13) received cisplatin/doxorubicin/methotrexate as perioperative chemotherapy and the remaining patients cisplatin/doxorubicin. After a median follow-up of 46.9 months (range, 32.8-61.1), the median recurrence-free survival was 58.7 months (range, 18.5-98.8) and the median OS 64.1 months (range, 25.6-102.6). Except for fever and chills, the only adverse event probably related to mifamurtide was pericarditis (n=1). Conclusions: Mifamurtide was well tolerated in a Greek osteosarcoma population, including patients older than 30 years. The small sample size and the non-comparative design do not allow drawing conclusions on the drug benefit in terms of survival.
Wild Animal Densities as Predictor of Cattle Disease Risks and Breed Types in South-Western Uganda
Authors: Nina Pius Mbuya , Mugisha Samuel , Herwig Leirs , Damme Patrick Van 
Published: February 2022

Abstract
Background
This study investigated the spatial distribution of wild ungulates that pastoralist communities perceive as culprits in the transmission of cattle diseases outside protected areas in south-western Uganda. Diseases are hypothesized as having influence on pastoralists’ choice of cattle breed types. Until the time of his study no information was available on spatial patterns of wild animal species and cattle breeds reared in Lake Mburo Conservation Area, and how diseases transmitted therein potentially influence cattle breed herd sizes.
Methods
Animal population survey was carried out on cattle and wild ungulate species along transect lines laid perpendicular to the northern boundary of Lake Mburo National Park (LMNP). Household survey generated data on costs of disease control through interview. Data were analyzed for unit cost of disease control at herd level and correlated with spatial distribution patterns of cattle breeds and wild ungulate populations.
Results
Our results show inverse association of distance away from LMNP with wild ungulate populations and the cost of disease control, which in turn favored higher abundance of Friesian cattle. Cost of disease control and cattle abortion incidences were much lower in rangelands far away from LMNP (R² = 0.965, P < 0.001). Mean population of indigenous cattle significantly decreased while those of exotic breed increased with increasing distance away from the park boundary.
Conclusion
Spatial distribution of wild ungulates in cattle grazing rangelands potentially influence cattle disease prevalence levels which had significant associated with spatial patterns of cattle breeds and breed herd sizes.
SunCatcher: Clonal Barcoding with qPCR Detection Enables Live Cell Functional Analyses to Study Cancer Heterogeneity, Progression, and Metastasis
Authors: McAllister Sandra , Guo Qiuchen , Spasic Milos , Maynard Adam , Goreczny Gregory , Bizuayehu Amanuel , Olive Jessica 
Published: February 2022

Abstract
Single cell tracing and “omics” methods are valuable research tools; however, current approaches do not easily enable live cell retrieval. That is a particular issue when further study of cells that were eliminated or otherwise not selected during experimentation could provide critical information. We report a clonal molecular barcoding method, called SunCatcher, that enables longitudinal tracking and functional analysis of live cells. Single cell-derived clonal populations are generated from complex cell populations, infected with a unique molecular barcode, and stocks of each barcoded clone (BC) are retained. BCs can be combined to create a version of the original cell population or to generate custom BC pools. We developed both quantitative PCR-based and next-generation sequencing methods for identifying and quantifying BCs in vitro and in vivo. We applied SunCatcher to various breast cancer cell lines. Heterogeneous pools of BCs reliably reproduced the proliferation and cancer progression rates of the original parental cell lines. Individual BCs, however, displayed a diversity of proliferation rates and molecular phenotypes that associated with their positive or negative selection in vivo. SunCatcher also enabled identification and quantification of early spontaneous metastases that were not detected by conventional methods. We envision that SunCatcher will serve as a reliable and widely accessible research and discovery tool.
An Analytical Approach of Error Detection and Correction for On-board Nano Satellite
Authors: Hasan Mahmudul , Islam Md. Motaharul 
Published: February 2022

Abstract
Nanosatellites are persistently progressing and creating global communication and data transmission over the world. It builds up a colossal request for more progressed and dependable frameworks that are able to perform faster and reliable information transmission. An approach has been distinguished as a reasonable plot for the anticipation of single bit and multiple bit errors that are influencing On-board Nanosatellites. In this paper, we have proposed an Analytical Approach of Error Detection and Correction for On-board Nanosatellite. We have planned the framework with three distinctive parts as a encoding, error checking, and decoding portion separately. It has created in such a way that amid information exchange from satellite to ground station to analyze six camera pictures at the same time with the assistance of FPGA and EDAC strategies. To extend the proficiency, we have presented the progressed turbo mechanics EDAC for distinctive transfer speeds of satellite communication and execution examination with the AWGN and Rayleigh channel. EDAC strategies codes are tried in MATLAB and appeared in graphical plots. This method is straightforward and accomplishes unwavering quality and exactness, compared to other comparable strategies.
VATS Resection of Anterior Mediastinal Disease: Superiority of Subxiphoid Approach Comparing to Lateral Intercostal Approach
Authors: Gengxu He , Yao Tong , zhao lei , geng hong , ji qiang , zuo kun , Yuanzhi Luo , Kai Zhou 
Published: February 2022

Abstract
Background & Aim: Optimal approaches for video-assisted thoracoscopic surgery (VATS) resection of anterior mediastinal diseases remain controversial. The present study was designed to compare subxiphoid approach with lateral intercostal approach, together with perioperative indices.Methods: Patients diagnosed with anterior mediastinal diseases between January 1, 2018 and October 31, 2019 were prospectively randomly assigned to two groups, to receive lateral intercostal approach vs. subxiphoid approach for VATS resection. Intraoperation PaCO2, SaO2, PaO2 and circulation changes were recorded. Perioperative outcomes, including clinical and surgical parameters such as operation time and blood loss, postoperative complication, neutrophil-to-lymphocyte ratio, and postoperative pain scores were compared. Results: A total 59 patients diagnosed with anterior mediastinal tumors or myasthenia gravis underwent VATS resection. 31 patients treated with subxiphoid approach and 28 patients treated with lateral intercostal approach had comparable baseline characteristics. Intraoperation PaCO2 was increased significantly whereas SaO2 remained stable in subxiphoid group. By contrast, PaCO2 was increased significantly whereas SaO2 was decreased in lateral intercostal group, and operations were more frequently interrupted due to hypoxia or circulation disturbance during thymus dissection in the lateral intercostal approach. Compared with lateral intercostal group, patients in subxiphoid group experienced less inflammation reaction, while yielded lower pain scores and shorter postoperative hospital stay. There were no significant differences in postoperative complications between the two groups. All these patients recovered well when discharged. There were no perioperative deaths. Conclusion: Subxiphoid approach may influence pulmonary and circulation lesser than lateral intercostal approach. Procedure may be safer and easier for subxiphoid approach, as an ideal choice for anterior mediastinal diseases.
The Impact of Air Quality on International Tourism Arrivals: A Global Panel Data Analysis
Authors: SU Yan , Lee Chien-Chiang 
Published: February 2022

Abstract
Using data from World Development Indicators (WDI), this research constructs panel data of 99 countries from 1996 to 2018 and uses a spatial econometric model to analyze the impact of air quality on international tourism arrivals. Findings present that Moran’s I values are significantly positive, indicating a strong positive spatial dependence in each country, and that poor air quality does have a negative impact on the number of tourist arrivals. The results of grouped data show that middle-income countries, low-income countries, high concentrations of PM2.5, and countries that less numbers of tourists have negative effects on tourist arrivals in neighboring countries at least at the 10% significance level. The contrary groups, however, have positive effects on tourist arrivals - that is, the influence of air quality on the number of tourist arrivals exhibits heterogeneity. In addition, tests of the interaction term show that countries with higher R&D intensity have better air quality and thus attract more tourists. Therefore, countries with poor air quality should improve the environment through international cooperation and undertake technology transfer, thus ultimately increasing the number of tourists.
Influence of Preventive Sex Education Programmes in Compulsory Secondary Education Students: a Descriptive Observational Study
Authors: Berutich Arturo Hidalgo , Cortes María Barbosa , Feria Esther Cárdenas , Rufete Margarita Carrillo , González Miguel Pedregal , Razquin Eduardo Perez , Arcos Esteban Delgado 
Published: February 2022

Abstract
Background: Sex education programmes conducted by health professionals and educators are essential for young people to adopt healthy habits and attitudes towards their sexuality. The Forma Joven Program, promoted by the Andalusian Regional Government's Ministry of Health and Families and Education, is a good example of this. The aim of the study is to determine if different “degrees of intervention” in the informative consultancies of the Forma Joven Program imply differences in knowledge and attitudes towards sexuality.Methods: This descriptive observational study analysed 27 Compulsory Secondary Education high schools in Huelva. These were included in the Program and belonged to a Primary Care Health District. Out of the 17 institutes initially selected because they met the inclusion criteria (4,256 students), finally 14 (3,596 students) participated. During the 2018/2019 school year, students from 3rd, 4th year of Secondary Compulsory Education (ESO), 1st, and 2nd Baccalaureate of the selected centres were asked to fill in a questionnaire of knowledge and attitudes towards sexuality. It collected variables such as age, sex, school year, institute, educational level and employment status of the father or mother and profile of the professional who delivers the counselling. Results: A total of 1.237 students completed the questionnaire, which represents a participation rate of 34.4%. The average age was 15.59 years (SD 1.26) and 39.9% were girls. In some evaluated questions, we found statistically significant differences between the groups with different levels of exposure to counselling and the acquisition of knowledge and attitudes towards students' sexuality, although in most of them no such differences were found. The results of this study suggest the importance of the quality of counselling over quantity. Some classic myths persist in relation to sexuality and in some situations, they can be decisive when adopting preventive measures to avoid risks related to pregnancy and contagion of STIs.Conclusions: A greater number of counselling sessions does not imply acquiring a higher level of knowledge or better attitudes towards sexuality. Perhaps the quality of the education is more important than the quantity of counselling sessions.
Intensive management disrupts belowground multi-trophic resources transfers in response to drought
Authors: Chomel Mathilde , Lavallee Jocelyn , Segura Nil Alvarez , Baggs Elizabeth , Caruso Tancredi , de Castro Francisco , Emmerson Mark , Magilton Matthew , Rhymes Jennifer , Stott Heather , de Vries Franciska , Johnson David , Bardgett Richard 
Published: February 2022

Abstract
Modification of soil food webs by historical land management may alter the response of ecosystem processes to climate extremes, but empirical support for this is limited and the mechanisms involved remain unclear. Here, we quantified how historical grassland management modifies transfers of recent photosynthate and soil nitrogen through plants and soil food web in response to drought, using in situ 13C and 15N pulse-labelling in paired intensively and extensively managed fields. We show that intensive management decreased plant carbon capture, its transfer through key components of food webs and soil respiration compared to extensive management. Drought only affected carbon transfer pathways in intensively managed grasslands, by increasing plant C assimilation but decreasing its transfer to plant roots, bacteria and Collembola. However, drought lowered the reduction of added nitrate to nitrous oxide in extensively managed grassland only. Our findings indicate that intensive management disrupts fluxes of recent photosynthates belowground, which impaired resistance of this process in response to drought. By contrast, extensive grassland management provides a greater potential to buffer impacts to drought by promoting the transfer of recent photosynthate belowground. Our work highlights that capture and rapid transfer of photosynthate through multitrophic networks is a key process for maintaining grassland resilience to drought.
Arterial injury mechanism of homocysteine after PCI in patients with premature coronary heart disease
Authors: Liu Mei , Yin Ya-Juan , Zheng Ming qi , Shi Xiao-Cui , Liu Da , Liu Dong-Yue , Liu Gang , Wei Mei 
Published: February 2022

Abstract
Background: One cause of mortality among young people in modern societies is coronary heart disease (CHD). Numerous studies have demonstrated an association between major adverse cardiac events (MACEs) and percutaneous coronary intervention (PCI) in patients with premature CHD, but the risk factors for MACEs after PCI are poorly understood. This study investigated the risk factors for premature CHD after PCI and compared the long-term prognosis of patients with premature CHD with or without MACEs after PCI. Homocysteine was found to be an independent risk factor for patients with early CHD. Previous animal studies have demonstrated that atorvastatin inhibits homocysteine-induced inflammation and that atorvastatin has preventive and therapeutic effects in patients with early CHD.Methods: A total of 1408 patients who underwent PCI were enrolled and divided into a MACE group and a non-MACE group according to the recurrence of cardiovascular events. Basic information and follow-up data of the patients were recorded, and risk factors affecting prognosis were analysed. Western blotting and reverse transcription polymerase chain reaction were used to identify that atorvastatin inhibited the expression of NF-κB, IL-1β, IL-6, and CRP inflammatory factors induced by homocysteine, thereby inhibiting the occurrence and development of atherosclerosis.Results: Univariate Cox proportional risk models showed that the homocysteine level is a risk factor for recurrent MACEs. Multivariate Cox regression analysis also suggested that homocysteine is an independent risk factor for MACEs. The receiver operating characteristic curve showed that Hcy was a strong factor for predicting the occurrence of MACEs in patients with premature CHD. Atorvastatin was found to inhibit the expression of NF-κB, IL-1β, IL-6, and CRP inflammatory factors induced by homocysteine and delay the occurrence of reactive oxygen species.Conclusions: Homocysteine is an independent risk factor for MACEs in patients with premature CHD after PCI. Atorvastatin can effectively antagonize the expression of NF-κB, IL-1β, IL-6, CRP and other inflammatory factors induced by homocysteine.
Lp (a): An Independent Predictive Marker In Diseases
Authors: Wu Mingyang , Liu Wenen , Xiang Zhongyuan 
Published: February 2022

Abstract
objective To investigate the serum lipoprotein (a)(Lp (a)) level and analyze the correlation between Lp (a) and sex, age, smoking, drinking, different diseases and other blood lipid indexes.Methods 7432 inpatients in Second Xiangya Hospital from 2013 to 2020 were selected, 8 items of medical records were collected, and statistic were analyzed by SSPS 24.0 software.Results The differences of LP (a) in different age groups and smoking or not groups were statistically significant (P < 0.05). There were noteworthy differences in Lp (a) between chronic kidney disease (CKD) group, atherosclerosis (AS) group and AS patients with other diseases compared with the control group. Significant differences were seen in myocardial infarction (MI) and stroke groups, but not for the diabetes mellitus (DM) group and the high blood pressure group with the control group. The correlation coefficients of LP (a) with apolipoprotein B-100 (apoB), cholesterol (CHO), high-density lipoprotein (HDL-C) and lowdensity lipoprotein (LDL-C) were 0.179, 0.132, 0.143 and 0.143, respectively. Linear regression analysis showed that LP (a) level was positively correlated with age, smoking or not, AS, MI , CKD,β=0.101, 0.042, 0.086, 0.119, 0.015, respectively.Conclusion LP (a) is related to apo-B, CHO, HDL-C and LDL-C t, AS, MI and CKD.
The study of the performance of a biological fuel cell: A progress towards the improvement of low electrical bioenergy output by using an amplification system
Authors: benghernit mohammed , kameche mostefa , zerhouni fatima zohra , krim fatima zohra , sahraoui tewfik , innocent christophe 
Published: February 2022

Abstract
A microbial fuel cell (MFC) has been conceived and constructed for the treatment of the sheep manure wastes and conversion them into clean sustainable renewable energy. The chemical oxygen demand (COD) removal of 10 days running MFC increased by 58.7% showing simultaneously an increase in the cell voltage. However, this MFC technology faces practical barriers since it produces low electric energy. A power management system including the MFC, operational amplifier, solar photovoltaic panel and a boost DC/DC converter, was elaborated in this respect. The low voltage output obtained from the MFC, was thus substantially increased by the amplifier prior to polarization by a photovoltaic module with solar radiation. The amplified voltage was thus sufficiently enough and in consequence, utilized to feed a light emitting diode. The low output voltage 0.5 V was simply harvested, successfully boosted up to approximately 2 V (i.e. 4 times higher) and effectively harnessed as a power supply. This novel application is very interesting to utilize the natural bioenergy contained in wastes to supply small electronic devices.
Toxicity of Perfluoroalkyl Substances (PFAS) Toward Embryonic Stages of Mahi-Mahi (Coryphaena Hippurus)
Authors: Gebreab Kiflom Y. , Benetti Daniel , Grosell Martin , Stieglitz John D. , Berry John P 
Published: February 2022

Abstract
Perfluoroalkyl substances (PFAS) are highly persistent organic pollutants that have been detected in a wide array of environmental matrices and, in turn, biota including humans and wildlife wherein they have been associated with a multitude of toxic, and otherwise adverse effects, including ecosystem impacts. In the present study, we developed a toxicity assay for embryonic stages of mahi-mahi (Coryphaena hippurus), as an environmentally relevant pelagic fish species, and applied this assay to the evaluation of the toxicity of “legacy” and “next-generation” PFAS including, respectively, perfluorooctanoic acid (PFOA) and several perfluoroethercarboxylic acids (PFECA). Acute embryotoxicity, in the form of lethality, was measured for all five PFAS toward mahi-mahi embryos with median lethal concentrations (LC50) in the micromolar range. Consistent with studies in other similar model systems, and specifically the zebrafish, embryotoxicity in mahi-mahi generally (1) correlated with fluoroalkyl chain length and hydrophobicity, i.e., log P, of PFAS, and thus, aligned with a role of uptake in the relative toxicity; and (2) increased with continuous exposure, suggesting a possible role of developmental stage specifically including a contribution of hatching (and loss of protective chorion) and/or differentiation of target systems (e.g., liver). Compared to prior studies in the zebrafish embryo model, mahi-mahi was significantly more sensitive to PFAS which may be related to differences in either exposure conditions (e.g., salinity) and uptake, or possibly differential susceptibility of relevant targets, for the two species. Moreover, when considered in the context of the previously reported concentration of PFAS within upper sea surface layers, and co-localization of buoyant eggs (i.e., embryos) and other early developmental stages (i.e., larvae, juveniles) of pelagic fish species to the sea surface, the observed toxicity potentially aligns with environmentally relevant concentrations in these marine systems. Thus, impacts on ecosystems including, in particular, population recruitment are a possibility. The present study is the first to demonstrate embryotoxicity of PFAS in a pelagic marine fish species, and suggests that mahi-mahi represents a potentially informative, and moreover, environmentally relevant, ecotoxicological model for PFAS in marine systems.