bluuebunny/crossref_metadata_2025_split huggingface dataset:
Berberine ameliorates aGVHD by gut microbiota remodelling, TLR4 signalling suppression and colonic barrier repairment for NLRP3 inflammasome inhibition
Authors: Zhao Yanna , Huang Jiefeng , Li Tianyi , Zhang Shuijuan , Wen Chengping , Wang Lipei 
Published: January 2022

AbstractBerberine (BBR), an isoquinoline alkaloid, is used to treat gastrointestinal disorders as an herbal medicine in China. The aim of this study was to investigate the anti‐inflammatory activities of BBR in a mouse model with acute graft‐versus‐host disease (aGVHD). Mice were intravenously injected with bone marrow cells from donors combined with splenocytes to develop aGVHD. The body weight, survival rate and clinical scores were monitored. Then the levels of inflammatory cytokines, histological changes (lung, liver and colon), colonic mucosal barrier and gut microbiota were analysed. Moreover, the toll‐like receptor 4 (TLR4)/myeloid differentiation primary response gene 88 (Myd88)/nuclear factor‐κB signalling pathway, NLRP3 inflammasome and its cytokines’ expressions were determined. The results showed that the gavage of BBR lessened GVHD‐induced weight loss, high mortality and clinical scores, inhibited inflammation and target organs damages and prevented GVHD‐indued colonic barrier damage. Additionally, BBR modulated gut microbiota, suppressed the activation of the TLR4 signaling pathway and inhibited NLRP3 inflammasome and its cytokine release. This study indicated that BBR might be a potential therapy for aGVHD through NLRP3 inflammasome inhibition.
Care processes and racial/ethnic differences in family reports of end‐of‐life care among Veterans: A mediation analysis
Authors: Kutney‐Lee Ann , Bellamy Scarlett L. , Ersek Mary , Medvedeva Elina L. , Smith Dawn , Thorpe Joshua M. , Brooks Carthon J. Margo 
Published: January 2022

AbstractBackgroundBereaved family members of racial/ethnic minority Veterans are less likely than families of White Veterans to provide favorable overall ratings of end‐of‐life (EOL) care quality; however, the underlying mechanisms for these differences have not been explored. The objective of this study was to examine whether a set of EOL care process measures mediated the association between Veteran race/ethnicity and bereaved families' overall rating of the quality of EOL care in VA medical centers (VAMCs).MethodsA retrospective, cross‐sectional analysis of linked Bereaved Family Survey (BFS), administrative and clinical data was conducted. The sample included 17,911 Veterans (mean age: 73.7; SD: 11.6) who died on an acute or intensive care unit across 121 VAMCs between October 2010 and September 2015. Mediation analyses were used to assess whether five care processes (potentially burdensome transitions, high‐intensity EOL treatment, and the BFS factors of Care and Communication, Emotional and Spiritual Support, and Death Benefits) significantly affected the association between Veteran race/ethnicity and a poor/fair BFS overall rating.ResultsPotentially burdensome transitions, high‐intensity EOL treatment, and the three BFS factors of Care and Communication, Emotional and Spiritual Support, and Death Benefits did not substantially mediate the relationship between Veteran race/ethnicity and poor/fair overall ratings of quality of EOL care by bereaved family members.ConclusionsThe reasons underlying poorer ratings of quality of EOL care among bereaved family members of racial/ethnic minority Veterans remain largely unexplained. More research on identifying potential mechanisms, including experiences of racism, and the unique EOL care needs of racial and ethnic minority Veterans and their families is warranted.
Integration of light and temperature signaling pathways in plants
Authors: Qi Lijuan , Shi Yiting , Terzaghi William , Yang Shuhua , Li Jigang 
Published: January 2022

AbstractAs two of the most important environmental factors, light and temperature regulate almost all aspects of plant growth and development. Under natural conditions, light is accompanied by warm temperatures and darkness by cooler temperatures, suggesting that light and temperature are tightly associated signals for plants. Indeed, accumulating evidence shows that plants have evolved a wide range of mechanisms to simultaneously perceive and respond to dynamic changes in light and temperature. Notably, the photoreceptor phytochrome B (phyB) was recently shown to function as a thermosensor, thus reinforcing the notion that light and temperature signaling pathways are tightly associated in plants. In this review, we summarize and discuss the current understanding of the molecular mechanisms integrating light and temperature signaling pathways in plants, with the emphasis on recent progress in temperature sensing, light control of plant freezing tolerance, and thermomorphogenesis. We also discuss the questions that are crucial for a further understanding of the interactions between light and temperature signaling pathways in plants.
Implementation problems and adaptations among organizations delivering school‐based dental sealant programs
Authors: AlEissa Eiman E. , Catania Joseph A. 
Published: January 2022

AbstractObjectivesAlthough research supports the effectiveness of school‐based dental sealant programs (SBDSPs) in increasing access to dental care and reducing dental caries, there is limited information on the implementation processes that impact success at the population level. This study assessed SBDSP program implementation in terms of problems (and adaptations) encountered during school and client (students) recruitment, client attendance, and workforce capacity. These four factors are necessary for reaching eligible youth.MethodsWe interviewed program personnel (n = 18) at five organizations delivering SBDSPs in Oregon in 2018. Qualitative analysis employed a directed content approach.ResultsSpecific implementation problems included the following: school recruitment: workforce limitations, school refusals, community objections; client recruitment: ineffective recruitment materials, poor parent/guardian engagement, consent form shortcomings, poor consent forms dissemination and return, low school cooperation; client attendance: poor school cooperation, physical barriers, scheduling conflicts; workforce capacity: insufficient size, high turnover. Participants reported that their respective organizations successfully adapted to resolve these implementation problems with some exceptions (e.g., insufficiencies in workforce capacity).ConclusionImplementation problems related to school and client recruitment, client attendance, and workforce capacity may impact SBDSP reach. Adaptations to address these problems provide a basis for specific program recommendations. Larger systemic and translational problems were also observed, and recommendations were made regarding organizational communication and the need for additional pre‐implementation planning and pilot testing.
The impact of diabetes on young transplant recipients: An American perspective
Authors: Loera Jackquelin M. , Barrett Spencer C. , Zhang Theodore S. , Anand Adrish , Awan Ahmed A. Y. , Murthy Bhamidipati V. R. , O'Mahony Christine A. , Goss John A. , Rana Abbas A. 
Published: January 2022

AbstractDespite advancements in diabetic care, diabetic kidney transplant recipients have significantly worse outcomes than non‐diabetics.AimOur study aims to demonstrate the impact of diabetes, types I and II, on American young adults (18–40 years old) requiring kidney transplantation.MethodsUsing the United Network for Organ Sharing database, we conducted a population cohort study that included all first‐time, kidney‐only transplant recipients during 2002–2019, ages 18–40 years old. Patients were grouped according to indication for transplant. Primary outcomes were cumulative all‐cause mortality and death‐censored graft failure. Death‐censored graft failure and patient survival at 1, 5, and 10 years were calculated via the Kaplan–Meier method. Multivariate Cox regression was used to assess for potential confounders.ResultsOf 42 466 transplant recipients, 3418 (8.1%) had end‐stage kidney disease associated with diabetes. At each time‐point, cumulative mortality was higher in diabetics compared to patients with non‐diabetic causes of renal failure. Conversely, cumulative graft failure was similar between the groups. Adjusted hazard ratios for all‐cause mortality and graft failure in diabetics were 2.99 (95% CI 2.67–3.35; p < .01) and 0.98 (95% CI 0.92–1.05, p < .01), respectively.ConclusionDiabetes mellitus in young adult kidney transplant recipients is associated with a nearly three‐fold increase in mortality, reflecting a relatively vulnerable patient population. Identifying the underlying causes of poor outcomes in this population should be a priority for future study.
Effect of Long-Term Classical Ballet Dance Training on Postactivation Depression of the Soleus Hoffmann-Reflex
Authors: Obata Hiroki , Kim GeeHee , Ogawa Tetsuya , Sekiguchi Hirofumi , Nakazawa Kimitaka 
Published: January 2022

Classical ballet dancing is a good model for studying the long-term activity-dependent plasticity of the central nervous system in humans, as it requires unique ankle movements to maintain ballet postures. The purpose of this study was to investigate whether postactivation depression is changed through long-term specific motor training. Eight ballet dancers and eight sedentary subjects participated in this study. The soleus Hoffmann reflexes were elicited at after the completion of a slow, passive dorsiflexion of the ankle. The results demonstrated that the depression of the soleus Hoffmann reflex (i.e., postactivation depression) was larger in classical ballet dancers than in sedentary subjects at two poststretch intervals. This suggests that the plastic change through long-term specific motor training is also expressed in postactivation depression of the soleus Hoffmann reflex. Increased postactivation depression would strengthen the supraspinal control of the plantarflexors and may contribute to fine ankle movements in classical ballet dancers.
Geochronology and geochemistry of the Huntington Formation, Olds Ferry terrane, Blue Mountains province, northern U.S. Cordillera: Implications for accreted terrane correlation and assembly
Authors: Ware Bryant , Tumpane Kyle P. , Kurz Gene A. , Schmitz Mark D. , Northrup Clyde J. 
Published: January 2022

AbstractThe Olds Ferry terrane is the more inboard of two accreted volcanic arc terranes in the late Paleozoic–early Mesozoic Blue Mountains province of the northern U.S. Cordillera. We present geologic, geochronologic, and geochemical data from the volcano-sedimentary Huntington Formation of the Olds Ferry arc that place the terrane within a firm temporal and tectonomagmatic context, and establish its identity as a fringing arc terrane along the Triassic to Early Jurassic Cordilleran margin. The Huntington Formation is divided into two unconformity-bounded informal members: a Norian (ca. 220 Ma) lower member comprising a sequence of mafic-intermediate volcanics, massive volcaniclastic breccias, and minor carbonates deposited unconformably onto the 237.7 Ma Brownlee pluton and intruded by the 210.0 Ma Iron Mountain pluton; and a Rhaetian through Pleinsbachian (&lt;210–187.0 Ma) upper member composed of massive conglomerates, abundant rhyodacite to rhyolite effusive and pyroclastic flows, and interlayered sandstone turbidites, deposited with angular unconformity onto the lower member. An erosional hiatus and regional tilting produced an angular unconformity separating the Huntington Formation from the overlying basal conglomerates of the late Early to Middle Jurassic Weatherby Formation of the Izee forearc basin transgressive onlap sequence. Huntington Formation volcanic rocks are isotopically enriched relative to depleted mantle and coeval igneous rocks in the outboard Wallowa terrane. A temporal evolution to more radiogenic 87Sr/86Sr ratios (0.7036–0.7057) and εNd values (+5.4 to +3.1) in the upper member volcanics suggests increasing involvement of continental-derived material in their petrogenesis. Precambrian xenocrystic zircons in both lower and upper member volcaniclastic rocks strongly support a proximal location of the Olds Ferry terrane to cratonal North America during much of its history. The chronology and tectonostratigraphic architecture of the Olds Ferry terrane allows its robust correlation to other fringing-arc terranes along the U.S. and Canadian Cordillera.
Are fluid inclusions in gypsum reliable paleoenvironmental indicators? An assessment of the evidence from the Messinian evaporites
Authors: Bigi D. , Lugli S. , Manzi V. , Roveri M. 
Published: January 2022

AbstractThe paleosalinity of water from which the gypsum precipitated during the Messinian salinity crisis is a controversial issue. Recent microthermometry studies on primary fluid inclusions in gypsum provided very low salinity values not compatible with precipitation from seawater, and suggested strong mixing between seawater and nonmarine waters enriched in calcium sulfate. We applied a new microthermometric protocol on gypsum crystals from nine Mediterranean sections that were experimentally stretched to measure a larger population of fluid inclusions. The results show salinities ranging from 9 to 238 wt‰ NaCl equivalent, largely falling within the evaporation path of normal seawater. The data from previous studies were obtained mostly from those fluid inclusions capable of nucleating a stable bubble after a weak stretching, which probably correspond to those having a lower salinity acquired through post-depositional crack-and-seal processes. Our data suggest instead that the primary gypsum precipitated from a marine brine, later modified by post-trapping processes during tectonics and exhumation.
Experimental evidence on the origin of Ca-rich carbonated melts formed by interaction between sedimentary limestones and mantle-derived ultrabasic magmas
Authors: Lustrino Michele , Luciani Natascia , Stagno Vincenzo , Narzisi Silvia , Masotta Matteo , Scarlato Piergiorgio 
Published: January 2022

Abstract
In this experimental study, we documented the formation of strongly ultrabasic and ultracalcic melts through the interaction of melilititic and basanitic melts with calcite. Three strongly to moderately SiO2-undersaturated volcanic rocks from the Bohemian Massif (central Europe) were mixed with 10, 30, and 50 wt% CaCO3 and melted at 1100, 1200, and 1300 °C at 2 kbar to evaluate the maximum amount of carbonate that can be assimilated by natural ultrabasic melts at shallow depths. Experiments revealed a surprisingly complete dissolution of the CaCO3, only rarely reaching carbonate saturation, with typical liquidus phases represented by olivine, spinel, melilite, and clinopyroxene. Only in the runs with the most SiO2-undersaturated compositions did abundant monticellite form instead of clinopyroxene. For all starting mixtures, strongly ultrabasic (SiO2 down to 15.6 wt%), lime-rich (CaO up to 43.6 wt%), ultracalcic (CaO/Al2O3 up to ~27) melt compositions were produced at 1200 and 1300 °C, with up to ~25 wt% dissolved CO2. When present, quenched olivine showed much higher forsterite content (Fo95–97) than olivine in the natural samples (Fo79–85). The two major results of this study are (1) silicate-carbonatite melt compositions do not necessarily imply the existence of carbonatitic components in the mantle, because they are also produced during limestone assimilation, and (2) Fo-rich olivines cannot be used to infer any primitive character of the melt nor high potential temperature (Tp).
Metformin in pregnancy and risk of adverse long-term outcomes: a register-based cohort study
Authors: Brand Kerstin M G , Saarelainen Laura , Sonajalg Jaak , Boutmy Emmanuelle , Foch Caroline , Vääräsmäki Marja , Morin-Papunen Laure , Schlachter Judith ,   CLUE Study Group, Hakkarainen Katja M , Korhonen Pasi 
Published: January 2022

IntroductionThis study aimed to investigate if maternal pregnancy exposure to metformin is associated with increased risk of long-term and short-term adverse outcomes in the child.Research design and methods This register-based cohort study from Finland included singleton children born 2004–2016 with maternal pregnancy exposure to metformin or insulin (excluding maternal type 1 diabetes): metformin only (n=3967), insulin only (n=5273) and combination treatment (metformin and insulin; n=889). The primary outcomes were long-term offspring obesity, hypoglycemia, hyperglycemia, diabetes, hypertension, polycystic ovary syndrome, and challenges in motor–social development. In a sensitivity analysis, the primary outcomes were investigated only among children with maternal gestational diabetes. Secondary outcomes were adverse outcomes at birth. Analyses were conducted using inverse- probability of treatment weighting (IPTW), with insulin as reference.Results  Exposure to metformin or combination treatment versus insulin was not associated with increased risk of long-term outcomes in the main or sensitivity analyses. Among the secondary outcomes, increased risk of small for gestational age (SGA) was observed for metformin (IPTW-weighted OR 1.65, 95% CI 1.16 to 2.34); increased risk of large for gestational age, preterm birth and hypoglycemia was observed for combination treatment. No increased risk was observed for neonatal mortality, hyperglycemia, or major congenital anomalies.Conclusions This study found no increased long-term risk associated with pregnancy exposure to metformin (alone or in combination with insulin), compared with insulin. The increased risk of SGA associated with metformin versus insulin suggests caution in pregnancies with at-risk fetal undernutrition. The increased risks of adverse outcomes at birth associated with combination treatment may reflect confounding by indication or severity.
Predictors of arterial stiffness in adolescents and adults with type 1 diabetes: a cross-sectional study
Authors: Love Kaitlin M , Horton William B , Patrie James T , Jahn Linda A , Hartline Lee M , Barrett Eugene J 
Published: January 2022

IntroductionIndividuals with type 1 diabetes have increased arterial stiffness compared with age-matched healthy controls. Our aim was to determine which hemodynamic and demographic factors predict arterial stiffness in this population.Research design and methodsCarotid-femoral pulse wave velocity (cfPWV) was examined in 41 young adults and adolescents with type 1 diabetes without microvascular complications. Two ordinary least squares regression analyses were performed to determine multivariate relationships between cfPWV (loge) and (1) age, duration of diabetes, sex, and hemoglobin A1c and (2) augmentation index (AIx), mean arterial pressure, flow-mediated dilation (FMD), and heart rate. We also examined differences in macrovascular outcome measures between sexes.ResultsAge, sex, and FMD provided unique predictive information about cfPWV in these participants with type 1 diabetes. Despite having similar cardiovascular risk factors, men had higher cfPWV compared with women but no differences were observed in other macrovascular outcomes (including FMD and AIx).ConclusionsOnly age, sex, and FMD were uniquely associated with arterial stiffness in adolescents and adults with uncomplicated type 1 diabetes. Women had less arterial stiffness and similar nitric oxide-dependent endothelial function compared with men. Larger, prospective investigation is warranted to determine the temporal order of and sex differences in arterial dysfunction in type 1 diabetes.
Innovative, enhanced community management of non-hypoxaemic chest-indrawing pneumonia in 2–59-month-old children: a cluster-randomised trial in Africa and Asia
Authors:   EMPIC Study Group
Published: January 2022

IntroductionThe WHO recommends oral amoxicillin for 2–59-month-old children with chest-indrawing pneumonia presenting at the health facility. Community-level health workers (CLHWs) are not allowed to treat these children when presented at the community level. This study aimed to evaluate whether CLHWs can safely and effectively treat children 2–59 months-old with chest indrawing with a 5-day course of oral amoxicillin in a few selected countries in Africa and Asia, especially when a referral is not feasible.MethodsWe conducted a prospective multicountry cluster-randomised, open-label, non-inferiority trial in rural areas of four countries (Bangladesh, Ethiopia, India and Malawi) from September 2016 to December 2018. Children aged 2–59 months having parents/caregivers reported cough and/or difficult breathing presenting to a CLHW were screened for enrolment. CLHWs in the intervention clusters assessed children for hypoxaemia and treated non-hypoxaemic chest-indrawing pneumonia with two times per day oral amoxicillin (50 mg/kg body weight per dose) for 5 days at the community level. CLHWs in the control clusters identified chest indrawing and referred them to a referral-level health facility for treatment. Study supervisors performed pulse oximetry in the control clusters except in Bangladesh. Children were assessed for the primary outcome (clinical treatment failure) up to day 14 after enrolment. The accuracy and impact of pulse oximetry by CLHWs in the intervention clusters were also assessed.ResultsIn 208 clusters, 1688 CLHWs assessed 62 363 children with cough and/or difficulty breathing. Of these, 4013 non-hypoxaemic 2–59-month-old children with chest-indrawing pneumonia were enrolled. We excluded 116 children from analysis, leaving 3897 for intention-to-treat analysis. In the intervention clusters, 4.3% (90/2081) failed treatment, including five deaths, while in the control clusters, 4.4% (79/1816) failed treatment, including five deaths. The adjusted risk difference was -0.01 (95% CI −1.5% to 1.5%), which satisfied the prespecified non-inferiority criterion. CLHWs correctly performed pulse oximetry in 91.1% (2001/2196) of cases in the intervention clusters.ConclusionsThe community treatment of non-hypoxaemic children with chest-indrawing pneumonia with 5-day oral amoxicillin by trained, equipped and supervised CLHWs is non-inferior to currently recommended facility-based treatment. These findings encourage a review of the existing strategy of community-based management of pneumonia.Trial registrationACTRN12617000857303; The Australian New Zealand Clinical Trials Registry.