bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Modified Allgöwer–Donati Suture Technique on Wound Cosmetics in Spinal Surgery
Authors: Li Erliang , Zhang Tong , Ma Qiong , Yan Kang , Zhang Xueliang , Wang Wenji , Liao Bo 
Published: February 2022

ObjectiveTo analyze the efficacy of modified Allgöwer–Donati suture (MADS) technique on cosmetic outcomes compared with vertical mattress suture (VMS) technique in spinal surgery wounds.MethodsThis randomized controlled trial was conducted at the First Hospital of Lanzhou University (Gansu, China) from September 2019 to August 2020. The patients were randomly divided into two groups, a VMS group and a MADS group, by staff not involved in the treatment using a computer‐based random number table program (no restrictions on age or sex). Both procedures were performed by the same group of physicians as well as assistants. All suture wounds were completed by the same person. The primary endpoint was the scar area, and the postoperative scar area was scored by the Patient and Observer Scar Scale Assessment (POSAS). The scar area was calculated by ImageJ software. The second outcome measure was wound complications, including poor wound healing, wound edge necrosis, and infection. The trial was recorded in the Chinese Clinical Trial Register on 18 August 2019 (ChiCTR1900024548).ResultsA total of 143 patients were included: 72 in the VMS group and 71 in the MADS group. There was no significant difference in their demographics in terms of age (49.71 ± 8.91 vs 50.15 ± 6.79 years, P = 0.737), sex (M/F, 30/41 vs 31/41, P = 0.923), suture time (3.39 ± 0.22 vs 3.47 ± 0.25 s/mm, P = 0.057), or body mass index (BMI, 23.88 ± 3.50 vs 24.05 ± 3.50, P = 0.765) for MADS to VMS. The postoperative scar area was compared between the two groups transversely on day 12, the MADS wound scars decreased by 58.95% (75,133.24/127,452.58). In the POSAS evaluation, after MADS treatment, surface area score decreased from 5 (4, 5) to 2 (2, 3) (P < 0.0001), observer's overall opinion from 5 (4, 5) to 3 (2, 3) (P < 0.0001), itching from 3 (3, 4) to 3 (2, 3) (P = 0.001), color from 4 (4, 5) to 3 (2–4) (P < 0.0001), stiffness 4 (3–4.75) to 3 (3, 4) (P < 0.0001), or thickness from 4 (3–5) to 4 (3, 4) (P = 0.004). In terms of overall opinion evaluation, the MADS showed a significant difference in observer's overall opinion to the VMS (5 (4, 5) vs 3 (2, 3), P < 0.0001) and in patient's overall opinion 5 (5, 6) to 3 (3, 4), (P < 0.0001). There was no significant statistical difference in poor wound healing (3 vs 0, P = 0.245), wound edge necrosis (3 vs 0, P = 0.245), and infection (1 vs 0, P = 1.000) with the MADS to the VMS.ConclusionThe results of this study show that the MADS effectively reduced the surgical scar area to 58.95% with no additional adverse events compared with that of the VMS in spine surgery.
All‐in‐one: a robust fluorescent fusion protein vector toolbox for protein localization and BiFC analyses in plants
Authors: Han Jingluan , Ma Kun , Li Huali , Su Jing , Zhou Lian , Tang Jintao , Zhang Shijuan , Hou Yuke , Chen Letian , Liu Yao‐Guang , Zhu Qinlong 
Published: February 2022

SummaryFluorescent tagging protein localization (FTPL) and bimolecular fluorescence complementation (BiFC) are popular tools for in vivo analyses of the subcellular localizations of proteins and protein–protein interactions in plant cells. The efficiency of fluorescent fusion protein (FFP) expression analyses is typically impaired when the FFP genes are co‐transformed on separate plasmids compared to when all are cloned and transformed in a single vector. Functional genomics applications using FFPs such as a gene family studies also often require the generation of multiple plasmids. Here, to address these needs, we developed an efficient, modular all‐in‐one (Aio) FFP (AioFFP) vector toolbox, including a set of fluorescently labelled organelle markers, FTPL and BiFC plasmids and associated binary vectors. This toolbox uses Gibson assembly (GA) and incorporates multiple unique nucleotide sequences (UNSs) to facilitate efficient gene cloning. In brief, this system enables convenient cloning of a target gene into various FFP vectors or the insertion of two or more target genes into the same FFP vector in a single‐tube GA reaction. This system also enables integration of organelle marker genes or fluorescently fused target gene expression units into a single transient expression plasmid or binary vector. We validated the AioFFP system by testing genes encoding proteins known to be functional in FTPL and BiFC assays. In addition, we performed a high‐throughput assessment of the accurate subcellular localizations of an uncharacterized rice CBSX protein subfamily. This modular UNS‐guided GA‐mediated AioFFP vector toolkit is cost‐effective, easy to use and will promote functional genomics research in plants.
Identification of ST1 reveals a selection involving hitchhiking of seed morphology and oil content during soybean domestication
Authors: Li Jun , Zhang Yuhang , Ma Ruirui , Huang Wenxuan , Hou Jingjing , Fang Chao , Wang Lingshuang , Yuan Zhihui , Sun Qun , Dong Xuehui , Hou Yufeng , Wang Ying , Kong Fanjiang , Sun Lianjun 
Published: February 2022

SummarySeed morphology and quality of cultivated soybean (Glycine max) have changed dramatically during domestication from their wild relatives, but their relationship to selection is poorly understood. Here, we describe a semi‐dominant locus, ST1 (Seed Thickness 1), affecting seed thickness and encoding a UDP‐D‐glucuronate 4‐epimerase, which catalyses UDP‐galacturonic acid production and promotes pectin biosynthesis. Interestingly, this morphological change concurrently boosted seed oil content, which, along with up‐regulation of glycolysis biosynthesis modulated by ST1, enabled soybean to become a staple oil crop. Strikingly, ST1 and an inversion controlling seed coat colour formed part of a single selective sweep. Structural variation analysis of the region surrounding ST1 shows that the critical mutation in ST1 existed in earlier wild relatives of soybean and the region containing ST1 subsequently underwent an inversion, which was followed by successive selection for both traits through hitchhiking during selection for seed coat colour. Together, these results provide direct evidence that simultaneously variation for seed morphology and quality occurred earlier than variation for seed coat colour during soybean domestication. The identification of ST1 thus sheds light on a crucial phase of human empirical selection in soybeans and provides evidence that our ancestors improved soybean based on taste.
Feeling the burn: Sunburn recall in staphylococcal scalded skin syndrome
Authors: Khanna Rayva , Guo Emily L , Kirkorian Anna Yasmine 
Published: February 2022

AbstractStaphylococcal scalded skin syndrome (SSSS) occurs primarily in infants and young children due to hematogenous dissemination of Staphylococcus aureus (S. aureus) exfoliative toxin resulting in painful erythema and superficial desquamation of the skin. Herein, we discuss a 7‐year‐old patient with SSSS in classic locations who additionally presented with photodistributed erythema without desquamation on the chest, arms, and back in locations of a recently healed sunburn. In this report, we discuss sunburn recall phenomenon as a result of SSSS.
Junctional epidermolysis bullosa with extensive lung involvement in three patients with a LAMB3 Mutation
Authors: Ahmed Fahad , Young Lisa R. , Perman Marissa J. 
Published: February 2022

AbstractJunctional epidermolysis bullosa (JEB) is characterized by skin and mucous membrane fragility leading to easy blistering. Blistering may be the result of multiple genetic mutations, including the LAMB3 gene encoding a subunit of laminin 332, an important protein in the basement membrane zone. The clinical presentation of JEB includes blistering and granulation tissue forming anywhere on the skin including around oral and nasal cavities, fingers, toes, and within mucous membranes such as the upper respiratory tract. Lung pathology associated with JEB is less commonly reported; we describe three children with LAMB3 pathogenic variants with extensive lung injury contributing to decline in clinical status and likely leading to their demise early in life.
Surgical deroofing in pediatric patients with hidradenitis suppurativa
Authors: Leszczynska Maria , Diaz Lucia Z. , Peña‐Robichaux Venessa 
Published: February 2022

AbstractHidradenitis suppurativa (HS) is a chronic, inflammatory follicular disorder that most commonly involves the intertriginous areas. It is characterized by recurrent nodules that may progress into deeper abscesses and sinus tracts. Treatment is challenging and often involves a combination of lifestyle modifications, medical therapies, and procedures to control symptoms. Deroofing is a tissue‐saving surgical technique that has been well studied in adults. In our clinical experience, it is an important adjunct therapy in pediatric patients with moderate‐to‐severe HS. We describe the step‐by‐step process of deroofing, including surgical clinical pearls specific to the pediatric population.
Excess iron promotes emergence of foamy macrophages that overexpress ferritin in the lungs of silicosis patients
Authors: Aloe Christian Anthony , Leong Tracy Li‐Tsein , Wimaleswaran Hari , Papagianis Paris Clarice , McQualter Jonathan Luke , McDonald Christine Faye , Khor Yet Hong , Hoy Ryan Francis , Ingle Aviraj , Bansal Vipul , Goh Nicole Soo Leng , Bozinovski Steven 
Published: February 2022

AbstractBackground and objectiveInhalation of high concentrations of respirable crystalline silica (RCS) can lead to silicosis. RCS contains varying levels of iron, which can cause oxidative stress and stimulate ferritin production. This study evaluated iron‐related and inflammatory markers in control and silicosis patients.MethodsA cohort of stone benchtop industry workers (n = 18) were radiologically classified by disease severity into simple or complicated silicosis. Peripheral blood and bronchoalveolar lavage (BAL) were collected to measure iron, ferritin, C‐reactive protein, serum amyloid A and serum silicon levels. Ferritin subunit expression in BAL and transbronchial biopsies was analysed by reverse transcription quantitative PCR. Lipid accumulation in BAL macrophages was assessed by Oil Red O staining.ResultsSerum iron levels were significantly elevated in patients with silicosis, with a strong positive association with serum ferritin levels. In contrast, markers of systemic inflammation were not increased in silicosis patients. Serum silicon levels were significantly elevated in complicated disease. BAL macrophages from silicosis patients were morphologically consistent with lipid‐laden foamy macrophages. Ferritin light chain (FTL) mRNA expression in BAL macrophages was also significantly elevated in simple silicosis patients and correlated with systemic ferritin.ConclusionOur findings suggest that elevated iron levels during the early phases of silicosis increase FTL expression in BAL macrophages, which drives elevated BAL and serum ferritin levels. Excess iron and ferritin were also associated with the emergence of a foamy BAL macrophage phenotype. Ferritin may represent an early disease marker for silicosis, where increased levels are independent of inflammation and may contribute to fibrotic lung remodelling.
Is Nordic elder care facing a (new) collaborative turn?
Authors: Vabø Mia , Zechner Minna , Stranz Anneli , Graff Lea , Sigurðardóttir Sigurveig H. 
Published: February 2022

AbstractNordic countries are known for their service‐based welfare states, which include basic health and social care for all older adults who have been formally assessed and found to need additional services. Facing fiscal constraints in the mid‐1990s, these countries endeavoured to create more cost‐effective care services that incorporated the doctrines of new public management (NPM). Overlapping NPM, steps have been taken to better integrate services and utilise the care capacity of a broader institutional and environmental set of actors. In this study, we draw attention to this call for collaborative and participatory modes of governance beyond NPM. We explore whether and how Nordic eldercare policies fit in to the framework and logic of new public governance (NPG). The data consist of 62 key government documents from five Nordic countries, representing the central features of eldercare policies over the past 10 years. Our content analysis is based on three conceptual lenses associated with NPG: service integration, service co‐production and cross‐sectoral co‐creation. The analysis shows that several policy issues are framed by the logic of NPG in all countries. Further research is needed to assess how these NPG measures are implemented and interacting with institutional arrangements of other public governance paradigms.
Hitchhiking into the future on a fly: Toward a better understanding of phoresy and avian louse evolution (Phthiraptera) by screening bird carcasses for phoretic lice on hippoboscid flies (Diptera)
Authors: Lee Leshon , Tan David J. X. , Oboňa Jozef , Gustafsson Daniel R. , Ang Yuchen , Meier Rudolf 
Published: February 2022

AbstractMany phoretic relationships between arthropods are understudied because of taxonomic impediments. We here illustrate for avian lice riding on hippoboscid flies how new natural history data on phoretic relationships can be acquired quickly with modern and cost‐effective barcoding techniques. Most avian lice are host‐specific, but some can arrive on new hosts by hitchhiking on hippoboscid flies that feed on bird blood. Our summary of the literature yielded 254 published records which we here show to belong to two large and 13 small interaction networks for birds, flies, and lice. In order to generate new records, we developed a protocol based on screening bird carcasses sourced with the help of citizen scientists. The inspection of 131 carcasses from Singapore led to the first record of a Guimaraesiella Eichler (Ischnocera: Philopteridae) louse species riding on Ornithoica momiyamai Kishida flies collected from a pitta carcass. Phoresy may explain why this louse species is now known from three phylogenetically disparate hosts (Pitta moluccensis (Müller), Ficedula zanthopygia (Hay); Pardaliparus elegans Lesson). A second new case of phoresy enhanced a large interaction network dominated by Ornithophila metallica (Schiner), a cosmopolitan and polyphagous hippoboscid fly species. Overall, we argue that many two‐ and three‐way phoretic relationships between arthropods (e.g., mites, pseudoscorpions, beetles, flies) can be resolved with cost‐effective large‐scale NGS barcoding, which can be used to partially overcome taxonomic impediments by pre‐sorting specimens for taxonomic revision.
The international experience of bacterial screen testing of platelet components with automated microbial detection systems: An update
Authors: Kamel Hany , Ramirez‐Arcos Sandra , McDonald Carl ,   ISBT Transfusion‐Transmitted Infectious Disease Bacterial Working Party Bacterial Subgroup
Published: February 2022

AbstractIn 2014, the bacterial subgroup of the Transfusion‐Transmitted Infectious Diseases working party of ISBT published a review on the International Experience of Bacterial Screen Testing of Platelet Components (PCs) with an Automated Microbial Detection System. The purpose of this review, which is focused on publications on or after 2014, is to summarize recent experiences related to bacterial contamination of PCs and the use of an automated culture method to safeguard the blood supply. We first reviewed septic transfusion reactions after PC transfusion as reported in national haemovigilance systems along with a few reports from various countries on bacterial contamination of blood products. Next, we reviewed PC automated culture protocols employed by national blood services in the United Kingdom, Australia, Canada and large blood collection organization and hospital transfusion services in the United States. Then, we acknowledged the limitations of currently available culture methodologies in abating the risks of transfusion‐transmitted bacterial infection, through a review of case reports. This review was neither meant to be critical of the literature reviewed nor meant to identify or recommend a best practice. We concluded that significant risk reduction can be achieved by one or a combination of more than one strategy. No one approach is feasible for all institutions worldwide. In selecting strategies, institutions should consider the possible impact on platelet components availability and entertain a risk‐based decision‐making approach that accounts for operational, logistical and financial factors.
Arthroscopic findings and long‐term outcomes in 76 sport horses with meniscal injuries (2008–2018)
Authors: Davis Joseph G. , García‐López José M. 
Published: February 2022

AbstractObjectiveTo report the findings and long‐term outcome of 76 sport horses with meniscal injury.Study designRetrospective case series.AnimalsSeventy‐six horses with 93 meniscal injuries in 85 stifles.MethodsMedical records of sport horses diagnosed with meniscal injury during arthroscopy were reviewed. Owner follow up was obtained via telephone interview ≥1.5 years postoperatively. Preoperative and intraoperative findings, and postoperative treatments, were analyzed for potential association with return to athletic performance.ResultsThe medial meniscus was involved in 82.8% of cases, with grade 1 injuries diagnosed in 76.3% of menisci. Overall, 85.5% of horses returned to athletic performance, with 40% returning to their previous level. The grade of meniscal injury was associated with long‐term outcome (P = .023). The presence of preoperative radiographic abnormalities (P = .259) or additional joint pathology (P = 1.00) was not associated with long‐term outcomes. Fifty‐nine stifles were treated with an orthobiologic: autologous conditioned serum, platelet‐rich plasma, or marrow‐derived mesenchymal stem cells. There was no association between the use of any orthobiologic and long‐term outcome (P = .394).ConclusionThis is the first report on long‐term outcome of sport horses with meniscal injuries following arthroscopic surgery. Overall, the long‐term prognosis was fair, with 40% of horses returning to their previous level of use. Severity of the meniscal injury was a prognostic indicator for return to work. The presence of radiographic abnormalities or additional joint pathology, or the use of orthobiologics, was not associated with long‐term outcome.Clinical significanceThese findings can help in prognostication for sport horses with meniscal injuries.
Convolutional Networks for Classification of Mortars
Authors: Lebbad Anderson , Clayton Garrett M. , Nataraj C. 
Published: February 2022

Abstract
The task of classifying unexploded mortars is critical in both humanitarian and military explosive ordnance disposal (EOD) operations. Classification needs to be completed quickly and accurately and is the first step toward disarming the ordnance because it provides information about the fuzing mechanism, or the stage in the arming cycle that the ordnance is currently in. To assist EOD technicians with mortar identification, this article presents an automated image-based algorithm and the database of images used in its development. The algorithm utilizes convolutional networks with variations to training to improve performance for ordnance found in varying states of disassembly. The classifier developed was found to be 98.5% accurate for these lab condition photos; future work will focus on more cluttered environments.
Impact Forming of AA5052-H32 Sheets With Friction Stir Spot Welds Using a Shock Tube and Failure Assessment
Authors: Barik Saibal Kanchan , Ganesh Narayanan R. , Sahoo Niranjan 
Published: February 2022

Abstract
In this present study, both the experimental and numerical investigations are carried out to understand the formability of AA 5052-H32 sheets of 1.5 mm thickness with friction stir spot weld (FSSW). A shock tube experimental facility is utilized in which a rigid hemispherical striker is propelled at a high velocity and deforms the FSSW sheets at high strain rates. In this analysis, the effect of different tool rotational speed and plunge depth on the FS spot welding outputs and forming outputs are understood. Furthermore, DEFORM-3D finite element (FE) code is used to perform FE simulation of both the FS spot welding and forming of the welded sheets interactively. During the forming analysis, a new strategy is followed to identify the rate-dependent mechanical properties that are incorporated during FE simulation. The tensile data obtained from the unwelded section of the sheet deformed using the shock tube is fit to the modified Johnson–Cook (MJC) model. In the case of the FS spot-welded region, a hardness-based multiplying factor is identified and used to obtain stress–strain data by fitting it to MJC model. The predicted temperature evolution during the FSSW is validated with the experimental data and a good correlation has been observed. The predicted material flow phenomenon gives an insight into the joint formation during FSSW. Various forming outputs such as deformation profile, crack pattern, and effective strain distribution predicted by MJC model in combination with Freudenthal damage model are compared with the experimental data, and the results have a fair agreement.
Validity of the Favero Assioma Duo Power Pedal System in Maximal-Effort Cycling Tests
Authors: Yeh Ming-Chia , Chen Wei-Han , Lu Sung-Ting , Chen Chi-Hsien , Liu Ya-Chen , Liu Chiang 
Published: February 2022

Purpose: The pedal-based power meter has its advantages, so it has become a popular monitoring tool in cycling. This study aimed to examine the validity of the Favero Assioma Duo power pedal system (FAD) in comparison with the SRM, which is considered the gold standard under maximal-effort cycling conditions, and a widely used cycling test, the 20-minute Functional Threshold Test. Methods: Fourteen male adolescent cyclists completed a series of cycling intervals including 5, 15, 30, 60, 240, 600, and 1200 seconds (20-min Functional Threshold Test) with their maximal-effort performance on 2 separate days. Power output data were collected from the FAD and the SRM for analysis. Results: Extremely strong correlations and excellent intraclass correlation coefficients (ICCs) were found between the power output values registered with the FAD and the SRM overall (r > .999, ICC = .996) and each power test (r > .98, ICC > .91). A low bias was found in power tests of longer durations (−3.2% at 240-s test, −3.3% at 600-s test, and −3.1% at 20-min Functional Threshold Test), while the bias augmented in shorter intervals (−2.7% at 5-s test, −3.6% at 15-s test, and −2.6% at 30-s test and −3.3% at 60-s test). A regression equation was proposed as y = −2.943 + 0.976x to diminish the bias (−0.2 W) with increased r value (>.98) and ICC (>.98). Conclusion: The FAD appears to be a valid tool for the measures of maximal-effort performance. The recorded power value reflects the true value with proposed regression equation.