bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lifestyle intervention during pregnancy in patients with gestational diabetes mellitus and the risk of neonatal hypoglycemia: A systematic review and meta-analysis
Authors: Wang Ya-Hai , Zhou Huan-Huan , Nie Zhibin , Tan Jingwang , Yang Zicheng , Zou Shengliang , Zhang Zheng , Zou Yu 
Published: August 2022

ObjectiveNeonatal hypoglycemia is a severe adverse consequence of infants born to mothers with gestational diabetes mellitus (GDM), which can lead to neonatal mortality, permanent neurological consequences, and epilepsy. This systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to explore the effect of lifestyle intervention during pregnancy in women with GDM on the risk of neonatal hypoglycemia.MethodsPubMed, Web of Science, Cochrane Library, CINAHL, and SPORTDiscus databases were searched by 1st April 2022. Data were pooled as the risk ratio (RR) with 95% CIs of neonatal hypoglycemia. Random-effects, subgroup analyses, meta-regression analysis, and leave-one-out analysis were conducted, involving 18 RCTs.ResultsPrenatal lifestyle intervention could significantly reduce the risk of neonatal hypoglycemia (RR: 0.73, 95% CI: 0.54–0.98, P = 0.037). Subgroup analysis further demonstrated that the reduced risk of neonatal hypoglycemia was observed only when subjects were younger than 30 years, initiated before the third trimester, and with dietary intervention. Meta-regression analysis revealed that the risk of neonatal hypoglycemia post lifestyle intervention was lower in mothers with lower fasting glucose levels at trial entry.ConclusionWe found that prenatal lifestyle intervention in women with GDM significantly reduced the risk of neonatal hypoglycemia. Only lifestyle intervention before the third trimester of pregnancy, or dietary intervention only could effectively reduce the risk of neonatal hypoglycemia. Future studies are required to explore the best pattern of lifestyle intervention and to determine the proper diagnostic criteria of GDM in the first/second trimester of pregnancy.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/#myprospero, PROSPERO, identifier: CRD42021272985.
Ubiquitin ligase RNF125 targets PD-L1 for ubiquitination and degradation
Authors: Wei Meng , Mo Yunhai , Liu Jialong , Zhai Jingtong , Li Huilong , Xu Yixin , Peng Yumeng , Tang Zhihong , Wei Tao , Yang Xiaopan , Huang Linfei , Shao Xiao , Li Jingfei , Zhou Li , Zhong Hui , Wei Congwen , Xie Qiaosheng , Min Min , Wu Feixiang 
Published: July 2022

As a critical immune checkpoint molecule, PD-L1 is expressed at significantly higher levels in multiple neoplastic tissues compared to normal ones. PD-L1/PD-1 axis is a critical target for tumor immunotherapy, blocking the PD-L1/PD-1 axis is recognized and has achieved unprecedented success in clinical applications. However, the clinical efficacy of therapies targeting the PD-1/PD-L1 pathway remains limited, emphasizing the need for the mechanistic elucidation of PD-1/PD-L1 expression. In this study, we found that RNF125 interacted with PD-L1 and regulated PD-L1 protein expression. Mechanistically, RNF125 promoted K48-linked polyubiquitination of PD-L1 and mediated its degradation. Notably, MC-38 and H22 cell lines with RNF125 knockout, transplanted in C57BL/6 mice, exhibited a higher PD-L1 level and faster tumor growth than their parental cell lines. In contrast, overexpression of RNF125 in MC-38 and H22 cells had the opposite effect, resulting in lower PD-L1 levels and delayed tumor growth compared with parental cell lines. In addition, immunohistochemical analysis of MC-38 tumors with RNF125 overexpression showed significantly increased infiltration of CD4+, CD8+ T cells and macrophages. Consistent with these findings, analyses using The Cancer Genome Atlas (TCGA) public database revealed a positive correlation of RNF125 expression with CD4+, CD8+ T cell and macrophage tumor infiltration. Moreover, RNF125 expression was significantly downregulated in several human cancer tissues, and was negatively correlated with the clinical stage of these tumors, and patients with higher RNF125 expression had better clinical outcomes. Our findings identify a novel mechanism for regulating PD-L1 expression and may provide a new strategy to increase the efficacy of immunotherapy.
Prediction of 5-year progression-free survival in advanced nasopharyngeal carcinoma with pretreatment PET/CT using multi-modality deep learning-based radiomics
Authors: Gu Bingxin , Meng Mingyuan , Bi Lei , Kim Jinman , Feng David Dagan , Song Shaoli 
Published: July 2022

ObjectiveDeep learning-based radiomics (DLR) has achieved great success in medical image analysis and has been considered a replacement for conventional radiomics that relies on handcrafted features. In this study, we aimed to explore the capability of DLR for the prediction of 5-year progression-free survival (PFS) in advanced nasopharyngeal carcinoma (NPC) using pretreatment PET/CT images.MethodsA total of 257 patients (170/87 patients in internal/external cohorts) with advanced NPC (TNM stage III or IVa) were enrolled. We developed an end-to-end multi-modality DLR model, in which a 3D convolutional neural network was optimized to extract deep features from pretreatment PET/CT images and predict the probability of 5-year PFS. The TNM stage, as a high-level clinical feature, could be integrated into our DLR model to further improve the prognostic performance. For a comparison between conventional radiomics and DLR, 1,456 handcrafted features were extracted, and optimal conventional radiomics methods were selected from 54 cross-combinations of six feature selection methods and nine classification methods. In addition, risk group stratification was performed with clinical signature, conventional radiomics signature, and DLR signature.ResultsOur multi-modality DLR model using both PET and CT achieved higher prognostic performance (area under the receiver operating characteristic curve (AUC) = 0.842 ± 0.034 and 0.823 ± 0.012 for the internal and external cohorts) than the optimal conventional radiomics method (AUC = 0.796 ± 0.033 and 0.782 ± 0.012). Furthermore, the multi-modality DLR model outperformed single-modality DLR models using only PET (AUC = 0.818 ± 0.029 and 0.796 ± 0.009) or only CT (AUC = 0.657 ± 0.055 and 0.645 ± 0.021). For risk group stratification, the conventional radiomics signature and DLR signature enabled significant difference between the high- and low-risk patient groups in both the internal and external cohorts (p &lt; 0.001), while the clinical signature failed in the external cohort (p = 0.177).ConclusionOur study identified potential prognostic tools for survival prediction in advanced NPC, which suggests that DLR could provide complementary values to the current TNM staging.
Apparent diffusion coefficient histogram analysis for differentiating solid ovarian tumors
Authors: Liu Renwei , Li Ruifeng , Fang Jinzhi , Deng Kan , Chen Cuimei , Li Jianhua , Wu Zhiqing , Zeng Xiaoxu 
Published: August 2022

ObjectiveTo evaluate the utility of apparent diffusion coefficient (ADC) histogram analysis to differentiate between three types of solid ovarian tumors: granulosa cell tumors (GCTs) of the ovary, ovarian fibromas, and high-grade serous ovarian carcinomas (HGSOCs).MethodsThe medical records of 11 patients with GCTs of the ovary (regions of interest [ROI-cs], 137), 61 patients with ovarian fibromas (ROI-cs, 161), and 14 patients with HGSOCs (ROI-cs, 113) confirmed at surgery and histology who underwent diffusion-weighted imaging were retrospectively reviewed. Histogram parameters of ADC maps (ADCmean, ADCmax, ADCmin) were estimated and compared using the Kruskal-WallisH test and Mann-Whitney U test. The area under the curve of receiver operating characteristic curves was used to assess the diagnostic performance of ADC parameters for solid ovarian tumors.ResultsThere were significant differences in ADCmean, ADCmax and ADCmin values between GCTs of the ovary, ovarian fibromas, and HGSOCs. The cutoff ADCmean value for differentiating a GCT of the ovary from an ovarian fibroma was 0.95×10-3 mm2/s, for differentiating a GCT of the ovary from an HGSOC was 0.69×10-3 mm2/s, and for differentiating an ovarian fibroma from an HGSOC was 1.24×10-3 mm2/s.ConclusionADCmean derived from ADC histogram analysis provided quantitative information that allowed accurate differentiation of GCTs of the ovary, ovarian fibromas, and HGSOCs before surgery.
SAMHD1 as a prognostic and predictive biomarker in stage II colorectal cancer: A multicenter cohort study
Authors: You Dingyun , Zhang Shuai , Yan Shan , Ding Yingying , Li Chunxia , Cheng Xianshuo , Wu Lin , Wang Weizhou , Zhang Tao , Li Zhenhui , He Yongwen 
Published: August 2022

BackgroundThe identification of high-risk population patients is key to the personalized treatment options for the stage II colorectal cancers. The use of proteomics in the prognosis of patients with stage II colorectal cancer remains unclear.MethodsUsing quantitative proteomics, we analyzed proteins that are differentially expressed in the tumor and adjacent normal tissues of 11 paired colorectal cancer patients with and without recurrence selected by a nested case-control design. Of the 21 identified proteins, we selected one candidate protein. The association of the corresponding gene of the selected protein with overall survival (OS) and adjuvant chemotherapy was analyzed using two independent cohorts of patients with stages II colorectal cancer.ResultsSterile α motif and histidine-aspartate domain-containing protein 1 (SAMHD1) was selected as the candidate biomarker. A group of 124 patients (12.5%) were stratified into SAMHD1-high subgroup. The 5-year OS rate of SAMHD1-high patients was lower than that of SAMHD1-low patients with stage II colorectal cancer (discovery cohort: hazard ratio [HR] = 2.89, 95% confidence interval [CI], 1.17-7.18, P = 0.016; validation cohort: HR = 2.25, 95% CI, 1.17-4.34, P = 0.013). The Cox multivariate analysis yielded similar results. In a pooled database, the 5-year OS rate was significantly different between patients with and without adjuvant chemotherapy among stage II SAMHD1-low tumors than in patients with stage II SAMHD1-high tumors (88% vs. 77%, P = 0.032).ConclusionsSAMHD1-high expression could help in identifying patients with stage II colorectal cancer with poor prognosis and less benefit from adjuvant chemotherapy.
Public health communication: Attitudes, experiences, and lessons learned from users of a COVID-19 digital triage tool for children
Authors: Michel Janet , Rehsmann Julia , Mettler Annette , Starvaggi Carl , Travaglini Nicola , Aebi Christoph , Keitel Kristina , Sauter Thomas C. 
Published: August 2022

BackgroundThe pandemic has made public health communication even more daunting because acceptance and implementation of official guidelines and recommendations hinge on this. The situation becomes even more precarious when children are involved. Our child-specific COVID-19 online forward triage tool (OFTT) revealed some of the public health communication challenges. We aimed to explore attitudes, experiences, and challenges faced by OFTT users and their families, in regard to public health recommendations.MethodsWe selected key informants (n = 20) from a population of parents, teachers, guardians, as well as doctors who had used the child-specific COVID-19 OFTT and had consented to a further study. Videos rather than face-face interviews were held. Convenience and quota sampling were performed to include a variety of key informants. Interviews were recorded, transcribed verbatim, and analyzed for themes.ResultsSeveral themes emerged, namely; (1) definition and expectations of high-risk persons, (2) quarantine instructions and challenges, (3) blurred division of responsibility between authorities and parents, (4) a novel condition and the evolution of knowledge, (5) definition and implications of socioeconomic status, (6) new normal and societal divisions, and (7) the interconnectedness of these factors-systems thinking.ConclusionAs the virus is evolving and circumstances are changing rapidly, the communication of public health to the different interest groups becomes, both an art and science, even more so when using a new technological communication channel: an OFTT. A myriad of interconnected factors seems to influence attitudes toward public health recommendations, which calls for systems thinking in public health communication.
Perceptions and preparedness toward tobacco cessation counseling amongst clinical medical students in Chongqing, Southwest China: A cross-sectional study
Authors: Yang Chuang , He Wenjin , Deng Ruihang , Giri Mohan , Dai Haiyun 
Published: August 2022

BackgroundMedical students play an indispensable role in providing smoking cessation counseling. Despite the rapid increase in tobacco use, there is little data on what Chinese medical students know or are taught about it. This study aims to investigate the relationship between medical students' tobacco education level, clinical experience, and tobacco cessation counseling (TCC) provided by medical students.MethodsThis cross-sectional study was carried out among clinical medical students of Chongqing medical university. An anonymous, self-administered questionnaire included items on demographic information, perceptions, and perceived preparedness, clinical medical students' self-reported level of education about alternative tobacco products, and traditional cigarettes. We assessed their perspectives toward TCC using a 5-point Likert scale. Descriptive and binary logistic regression analyses were carried out.ResultsA total of 1,263 medical students completed the questionnaire. The majority of students (85%) expressed a willingness to provide TCC to patients in need. However, only half of the students stated unequivocally that they knew some ways and methods of tobacco cessation, while 18% stated that they did not know methods of tobacco cessation. Tobacco education and clinical experience were significantly associated with the ability to provide TCC. Our findings revealed that students with more clinical experience (undergraduates: B = 0.326, P &lt; 0.001; postgraduates: B = 0.518, P &lt; 0.001) were significantly more likely to have a greater self-reported comprehensive ability to provide TCC.ConclusionTobacco education and clinical experience can enhance the ability of medical students to provide smoking cessation counseling. There is a need to focus on alternative tobacco products with changing times, and curriculum planners should collaborate to incorporate comprehensive tobacco prevention and cessation training into the medical school curriculum.
Maternal serum zinc level is associated with risk of preeclampsia: A systematic review and meta-analysis
Authors: Jin Senjun , Hu Chaozhou , Zheng Yanmei 
Published: August 2022

BackgroundPreeclampsia (PE) is a multi-organ syndrome that onsets in the second half of pregnancy. It is the second leading cause of maternal death globally. The homeostasis of zinc (Zn) levels is important for feto-maternal health.ObjectiveWe aimed to collect all studies available to synthesize the evidence regarding the association between maternal Zn levels and the risk of preeclampsia.MethodsA systematic review and meta-analysis was conducted via searching seven electronic databases [PubMed, Web of Science, Embase, African Journals Online (AJOL), ClinicalTrial.gov, and two Chinese databases: Wanfang and Chinese National Knowledge Infrastructure, CNKI]. Studies reporting maternal serum Zn levels in pregnant women with or without preeclampsia were included. Eligible studies were assessed through Newcastle-Ottawa Scale (NOS) and the meta-analysis was performed via RevMan and Stata. The random-effects method (REM) was used for the meta-analysis with 95% confidence interval (CI). The pooled result was assessed using standard mean difference (SMD). The heterogeneity test was carried out using I2 statistics, and the publication bias was evaluated using Begg's and Egger's test. Meta-regression and sensitivity analysis was performed via Stata software.ResultsA total of 51 studies were included in the final analysis. 6,947 participants from 23 countries were involved in our study. All studies went through the quality assessment. The pooled results showed that maternal serum Zn levels were lower in preeclamptic women than in healthy pregnant women (SMD: −1.00, 95% CI: −1.29, −0.70). Sub-group analysis revealed that geographical, economic context, and disease severity may further influence serum Zn levels and preeclampsia.LimitationsThere are significant between-study heterogeneity and publication bias among included studies.ConclusionsA lower level of maternal Zn was associated with increased risks of preeclampsia. The associations were not entirely consistent across countries and regions worldwide.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?RecordID=337069, Identifier: CRD42022337069
Influence of acetate- vs. lactate-containing fluid bolus therapy on acid-base status, electrolytes, and plasma lactate in dogs
Authors: Klein-Richers Ute , Heitland Annika , Hartmann Katrin , Dörfelt René 
Published: July 2022

ObjectiveAcetate- and lactate-containing fluids influence the acid-base and electrolyte status. This prospective, randomized, clinical study compared two balanced crystalloid solutions regarding their influence on acid-base status, electrolytes, and lactate values, when given to dogs as a resuscitation bolus of 30 mL/kg.Material and methodsOne hundred client-owned dogs presenting to the emergency service with signs of fluid deficits were randomly assigned to receive an intravenous bolus of 30 mL/kg of either a lactate- (LAC), or an acetate-containing solution (ACET). Before and after the bolus, vital parameters were assessed, and a venous blood gas analysis was performed.ResultsBoth solutions performed equally well in decreasing the heart rate (ACET: −10 ± 27 bpm, LAC: −12 ± 30 bpm;p= 0.737). The acetate-containing solution caused a significant decrease in plasma lactate levels (p= 0.016), anion gap (p&lt; 0.001), and potassium (p&lt; 0.001), and a significant increase in chloride (p&lt; 0.001), and ionized calcium (p= 0.014). The lactate-containing solution caused a significant decrease in anion gap (p&lt; 0.001), sodium (p= 0.016), and potassium (p= 0.001), and a significant increase in chloride (p&lt; 0.001). ACET causes a stronger decrease in plasma lactate (p= 0.015), sodium (p= 0.039), potassium (p= 0.006), and an increase in chloride (p&lt; 0.001), and ionized calcium (p= 0.016) compared to LAC.ConclusionBoth solutions caused mild changes in electrolyte concentrations and had minor influence on acid-base status when used for bolus therapy in dogs with fluid deficits. Further studies are needed to evaluate their influence on acid-base status, lactate, and electrolytes when used in larger volumes and for a longer time span.
ORIENTASI PASAR DAN BUDAYA ORGANISASI DALAM MEMPENGARUHI INOVASI DAN KINERJA PEMASARAN
Authors: Putri Viajeng Purnama , Arifiani Ratya Shafira 
Published: August 2022

Appropriate market orientation and a strong organizational culture are required to improve marketing performance by allowing product innovations to be created in accordance with business needs, which have an impact on marketing performance. This research was conducted on Small and Medium Enterprises (SMEs) in Malang City, where SMEs have an important role in the economy in Indonesia. The focus of this research is to see if market orientation has an impact on innovation, organizational culture has an impact on innovation, innovation has an impact on marketing performance, and the role of innovation in mediating market orientation on marketing performance and organizational culture on marketing performance. The main objective of this study is on the owner of a small firm in Malang City, as measured by market orientation, organizational culture, innovation, and marketing performance. Explanatory research is used in this work, which takes a quantitative method. The findings show that market orientation influences innovation, that organizational culture influences innovation, that marketing performance is influenced by market orientation, organizational culture, and innovation, and that marketing performance is mediated by market orientation and organizational culture..
Edukasi Pencegahan COVID-19 di Lingkungan Rumah Tangga di Yayasan Hati Mama Jimbaran Bali
Authors: Ariyanti Kadek Sri 
Published: July 2022

The Covid-19 pandemic has had a huge impact on children. This is a big concern for parents. Prevention of Covid-19 in children needs to be done so that the condition of the child remains healthy. Research conducted by Ariyanti (2020) in Gadungan Village Tabanan Bali shows that most households have implemented Covid-19 prevention behaviors well. However, it is still lacking in the implementation of regular disinfectants and regular sports activities. Better education is needed for families to increase activities to prevent transmission of Covid-19 in the household environment, especially in children.
The activity carried out was education on preventing Covid-19 in children in the household environment using leaflet media from the Ministry of Health. The targets are foster parents at the Hati Mama Jimbaran Foundation in Bali. This is followed by a health check on each family member and the provision of masks, multivitamins, hand soap and hand sanitizer.After the Covid-19 prevention education was carried out in the household environment, parents and foster children at the orphanage were committed to maintaining health protocols by: washing hands using soap and running water, using masks and hand sanitizers, maintaining distance and staying away from crowds, do physical activity regularly and eat nutritious food and take multivitamins.
Edukasi Pencegahan COVID-19 di Masyarakat Adat Pantai Kelating Kecamatan Tabanan
Authors: Dewi Putu Adi Cahya 
Published: July 2022

The Covid-19 pandemic has had a major impact on society, especially the Balinese, who are thick with their traditions. Prevention of Covid-19 in Balinese communities needs to be done so that healthy conditions in the community during the pandemic are maintained. Research conducted by Ariyanti (2020) at Gadungan Village of Tabanan Bali shows that most people have implemented Covid-19 prevention behaviors well. However, it is still lacking in the implementation of regular disinfectants and sports activities. Better education is needed for the community, especially in Balinese to increase of activities to prevent the spread of Covid-19.
The activity carried out was education on preventing Covid-19 for Balinese who were undergoing a ceremony to welcome the Nyepi day of Caka Year 1944 at Kelating Beach, Tabanan District, using leaflet media from the Ministry of Health. The target is Balinese who are present at Kelating Beach, Tabanan District. This is followed by a health check and the provision of masks, multivitamins, hand soap and hand sanitizer.
After the Covid-19 prevention education was carried out, they committed to maintaining health protocols by: washing hands using soap and running water, using masks and hand sanitizers, maintaining distance and away from crowds, doing physical activity regularly and eating. nutritious food and also take a multivitamin.