bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ginsenoside Rb2 Alleviated Atherosclerosis by Inhibiting M1 Macrophages Polarization Induced by MicroRNA-216a
Authors: Wang Shuting , Yang Shujun , Chen Yu , Chen Yutong , Li Rongxia , Han Shuang , Kamili Adalaiti , Wu Yiyi , Zhang Weili 
Published: January 2022

Introduction: Atherosclerosis is a chronic disease characterized by the inflammatory process and lipid depositions. We previously reported that microRNA-216a (miR-216a) can accelerate the progression of atherosclerosis by promoting the polarization of M1 pro-inflammatory phenotype. Ginsenoside Rb2 (Rb2), the major pharmacologically active compound extracted from ginseng, has a high affinity to miR-216a. In this study, we aimed to investigate whether Rb2 can counteract the effect of miR-216a in macrophages to ameliorate atherosclerosis.Methods: The apolipoprotein E deficiency (ApoE−/−) mice model was chronically infected with miR-216a adenovirus via the tail vein and then intraperitoneally injected with Rb2. The plaque lesion area and stability of thoracic aorta were examined. The human myeloid leukemia mononuclear cells (THP-1) or human peripheral blood mononuclear cells (PBMCs) were cultured in vitro, transfected with miR-216a mimics, and treated with Rb2 to explore the mechanisms of Rb2 on the polarization of M1 macrophages, inflammatory process, and lipid accumulation.Results: In the atherosclerotic ApoE−/− mice model, miR-216a greatly increased en face aortic lesion area of the thoracic aorta, lipid accumulation, and M1 macrophages infiltration in plaques, whereas these effects of miR-216a on atherosclerosis burden were significantly alleviated by Rb2 treatment. In the in vitro THP-1 model, the flow cytometry experiment showed that Rb2 treatment inhibited miR-216a–mediated polarization of M1 macrophages characterized by the surface marker CD86 expression but had no effects on M2 polarization characterized by the surface marker CD206 expression. Mechanistically, Rb2 suppressed the miR-216a–mediated inflammatory response through the Smad3/nuclear factor kappa B inhibitor alpha pathway. Moreover, Rb2 reduced the lipid uptake and promoted cholesterol efflux by counteracting the effects of miR-216a in the THP-1–derived foam cells and in the PBMC-derived foam cells under the oxidized low-density lipoproteins.Conclusion: Our findings indicated that Rb2 might be a potential therapeutic molecule for atherosclerosis by attenuating the atherosclerosis plaque lesion, lipid accumulation, and M1 macrophages polarization by targeting miR-216a. Given that accumulation of foam cells in the intima takes place chronically, the role of Rb2 in atherosclerosis progression needs further investigation.
Sex Hormone–Dependent Lipid Mediator Formation in Male and Female Mice During Peritonitis
Authors: Troisi Fabiana , Pace Simona , Jordan Paul M. , Meyer Katharina P. L. , Bilancia Rossella , Ialenti Armando , Borrelli Francesca , Rossi Antonietta , Sautebin Lidia , Serhan Charles N. , Werz Oliver 
Published: January 2022

Introduction: Sex differences in inflammation are obvious and contribute to divergences in the incidence and severity of inflammation-related diseases that frequently preponderate in women. Lipid mediators (LMs), mainly produced by lipoxygenase (LOX) and cyclooxygenase (COX) pathways from polyunsaturated fatty acids (PUFAs), regulate all stages of inflammation. Experimental and clinical studies revealed sex divergences for selected LM pathways without covering the entire LM spectrum, and only few studies have addressed the respective role of sex hormones. Here, we performed the comprehensive LM profile analysis with inflammatory peritoneal exudates and plasma from male and female mice in zymosan-induced peritonitis to identify the potential sex differences in LM biosynthesis during the inflammatory response. We also addressed the impact of sex hormones by employing gonadectomy.Methods: Adult male and female CD1 mice received intraperitoneal injection of zymosan to induce peritonitis, a well-established experimental model of acute, self-resolving inflammation. Mice were gonadectomized 5 weeks prior to peritonitis induction. Peritoneal exudates and plasma were taken at 4 (peak of inflammation) and 24 h (onset of resolution) post zymosan and subjected to UPLC–MS-MS–based LM signature profiling; exudates were analyzed for LM biosynthetic proteins by Western blot; and plasma was analyzed for cytokines by ELISA.Results: Pro-inflammatory COX and 5-LOX products predominated in the peritoneum of males at 4 and 24 h post-zymosan, respectively, with slightly higher 12/15-LOX products in males after 24 h. Amounts of COX-2, 5-LOX/FLAP, and 15-LOX-1 were similar in exudates of males and females. In plasma of males, only moderate elevation of these LMs was apparent. At 4 h post-zymosan, gonadectomy strongly elevated 12/15-LOX products in the exudates of males, while in females, free PUFA and LOX products were rather impaired. In plasma, gonadectomy impaired most LMs in both sexes at 4 h with rather up-regulatory effects at 24 h. Finally, elevated 15-LOX-1 protein was evident in exudates of males at 24 h which was impaired by orchiectomy without the striking impact of gonadectomy on other enzymes in both sexes.Conclusions: Our results reveal obvious sex differences and roles of sex hormones in LM biosynthetic networks in acute self-resolving inflammation in mice, with several preponderances in males that appear under the control of androgens.
A Dynamic Statistical Subseasonal Forecast Model for OLR Over Tropical Pacific Region
Authors: Wang Kuo , Fan Gao-Feng , Feng Guo-Lin 
Published: January 2022

How to improve the subseasonal forecast skills of dynamic models has always been an important issue in atmospheric science and service. This study proposes a new dynamical-statistical forecast method and a stable components dynamic statistical forecast (STsDSF) for subseasonal outgoing long-wave radiation (OLR) over the tropical Pacific region in January-February from 2004 to 2008. Compared with 11 advanced multi-model ensemble (MME) daily forecasts, the STsDSF model was able to capture the change characteristics of OLR better when the lead time was beyond 30 days in 2005 and 2006. The average pattern correlation coefficients (PCC) of STsDSF are 0.24 and 0.16 in 2005 and 2006, while MME is 0.10 and 0.05, respectively. In addition, the average value of PCC of the STsDSF model in five years is higher than MME in 7–11 pentads. Although both the STsDSF model and MME show a similar temporal correlation coefficient (TCC) pattern over the tropical Pacific region, the STsDSF model error grows more slowly than the MME error during 8–12 pentads in January 2005. This phenomenon demonstrates that STsDSF can reduce dynamical model error in some situations. According to the comparison of subseasonal forecasts between STsDSF and MME in five years, STsDSF model skill depends strictly on the predictability of the dynamical model. The STsDSF model shows some advantages when the dynamical model could not forecast well above a certain level. In this study, the STsDSF model can be used as an effective reference for subseasonal forecast and could feasibly be used in real-time forecast business in the future.
Numerical Study on Water Sealing Effect of Freeze-Sealing Pipe-Roof Method Applied in Underwater Shallow-Buried Tunnel
Authors: Hong Zequn , Zhang Jun , Han Lei , Wu Yuanhao 
Published: January 2022

The freezing-sealing pipe-roof method is a new presupporting technique, which fully combines the advantages of pipe-roof method and artificial ground-freezing method, and can adapt to the construction needs of underground projects in complex and sensitive strata. After the Gongbei Tunnel of Hong Kong–Zhuhai–Macao Bridge, this method will be applied for the first time in an underwater shallow-buried railroad tunnel, and there are still many urgent problems to be solved. In this article, based on the field situation and the preliminary design scheme, a convective heat transfer model under complex boundary conditions was first established. Then, the development of frozen wall thickness during the active freezing period was solved by numerical simulation for three different pipe filling modes, and the cloud map of temperature distribution in the whole section is analyzed. After that, the moving state of river water was characterized by different heat transfer coefficients, and the weakening effect of flow velocity on the top freezing wall was studied. Finally, six critical water sealing paths were selected, and the temperature differences of the frozen curtain were calculated. The results show that the mode with interval concrete filling can form a reliable frozen curtain within the scheduled time, whereas the nonfilling mode cannot achieve the water sealing requirement. River water has a large effect on the temperature at the boundary of jacking pipe and almost no effect on the center of the jacked pipe. It takes approximately 15 days from the frozen soil covering the pipe wall to reach the designed thickness, and the freezing effect of empty pipe lags approximately 28 days compared with that of solid pipe, which requires targeted enhancement measures in field projects.
Semi-automated Detection of the Timing of Respiratory Muscle Activity: Validation and First Application
Authors: Rodrigues Antenor , Janssens Luc , Langer Daniel , Matsumura Umi , Rozenberg Dmitry , Brochard Laurent , Reid W. Darlene 
Published: January 2022

Background: Respiratory muscle electromyography (EMG) can identify whether a muscle is activated, its activation amplitude, and timing. Most studies have focused on the activation amplitude, while differences in timing and duration of activity have been less investigated. Detection of the timing of respiratory muscle activity is typically based on the visual inspection of the EMG signal. This method is time-consuming and prone to subjective interpretation.Aims: Our main objective was to develop and validate a method to assess the respective timing of different respiratory muscle activity in an objective and semi-automated manner.Method: Seven healthy adults performed an inspiratory threshold loading (ITL) test at 50% of their maximum inspiratory pressure until task failure. Surface EMG recordings of the costal diaphragm/intercostals, scalene, parasternal intercostals, and sternocleidomastoid were obtained during ITL. We developed a semi-automated algorithm to detect the onset (EMG, onset) and offset (EMG, offset) of each muscle’s EMG activity breath-by-breath with millisecond accuracy and compared its performance with manual evaluations from two independent assessors. For each muscle, the Intraclass Coefficient correlation (ICC) of the EMG, onset detection was determined between the two assessors and between the algorithm and each assessor. Additionally, we explored muscle differences in the EMG, onset, and EMG, offset timing, and duration of activity throughout the ITL.Results: More than 2000 EMG, onset s were analyzed for algorithm validation. ICCs ranged from 0.75–0.90 between assessor 1 and 2, 0.68–0.96 between assessor 1 and the algorithm, and 0.75–0.91 between assessor 2 and the algorithm (p &lt; 0.01 for all). The lowest ICC was shown for the diaphragm/intercostal and the highest for the parasternal intercostal (0.68 and 0.96, respectively). During ITL, diaphragm/intercostal EMG, onset occurred later during the inspiratory cycle and its activity duration was shorter than the scalene, parasternal intercostal, and sternocleidomastoid (p &lt; 0.01). EMG, offset occurred synchronously across all muscles (p ≥ 0.98). EMG, onset, and EMG, offset timing, and activity duration was consistent throughout the ITL for all muscles (p &gt; 0.63).Conclusion: We developed an algorithm to detect EMG, onset of several respiratory muscles with millisecond accuracy that is time-efficient and validated against manual measures. Compared to the inherent bias of manual measures, the algorithm enhances objectivity and provides a strong standard for determining the respiratory muscle EMG, onset.
From Local Concerns to Global Challenges: Continuity and Change in Sub-state “Green Nationalism”
Authors: Hau Mark F. 
Published: January 2022

Issues related to anthropogenic climate change such as global warming, fossil fuel emissions, and renewable energy have emerged as some of the most important and pertinent political questions today. While the role of the state in the Anthropocene has been explored in academia, there is a severe dearth of research on the relationship between climate change and nationalism, especially at the sub-state level. This paper builds on the concept of “green nationalism” among sub-state nationalist parties in European minority nations. Using a multimodal analysis of selected European Free Alliance (EFA) campaign posters from the past 30 years, the article explores an extensive “frame bridging” where minority nationalist political actors actively seek to link environmental issues to autonomy. Although there is an apparent continuity in minority nationalist support for green policies, earlier initiatives focused on preservation of local territory while EFA parties today frame climate change as a global challenge that requires local solutions, which only they can provide. The frame bridging between territorial belonging and progressive politics has lead to the emergence of an environmentally focused, minority nationalist agenda that advocates for autonomy in order to enact more ambitious green policies, or “green nationalism”. This shows that nationalism in the right ideological environment can be a foundation for climate action, as minority nationalist actors base their environmentally focused agenda to address the global climate crisis precisely on their nationalist ideology.
Influence of Psychological Factors in Breast and Lung Cancer Risk – A Systematic Review
Authors: Pereira Maria Angelina , Araújo António , Simões Mário , Costa Catarina 
Published: January 2022

Introduction: In 2020, according to the Global Cancer Observatory, nearly 10 million people died of cancer. Amongst all cancers, breast cancer had the highest number of new cases and lung cancer had the highest number of deaths. Even though the literatures suggest a possible connection between psychological factors and cancer risk, their association throughout studies remains inconclusive. The present systematic review studied the connection between psychological factors and the risk of breast and lung cancer, prior to a cancer diagnosis. The psychological factors of trauma, grief, and depression were studied.Methods: The current systematic review was carried out across multiple databases in two phases, an initial exploratory research in June 2020, refined with a second electronic research in December 2020. The inclusion criteria included studies describing the association between trauma, posttraumatic stress disorder (PTSD), grief, and depression with breast and lung cancer risk. The psychological data collection must have been carried out prior to a confirmed breast or lung cancer diagnosis, and accessed through self-report measures, questionnaires, clinical interviews, or clinical diagnoses. Study reports had to contain information about the incidence of cancer and effect size. The exclusion criteria were studies in which psychological factors were collected after cancer diagnosis.Results and Conclusion: A total of 26 studies were included. Although non-consensual, the findings from the present systematic review suggest that, in addition to the known risk factors, psychological factors may play an important role in the etiology of both breast and lung cancer. To include psychological factors as a variable that affects cancer development may be fundamental to opening new avenues for prevention and intervention.Systematic Review Registration: [www.ClinicalTrials.gov], identifier [CRD42020209161].
Altered Effective Brain Connectivity During Habituation in First Episode Schizophrenia With Auditory Verbal Hallucinations: A Dichotic Listening EEG Study
Authors: Zheng Leilei , Yan Weizheng , Yu Linzhen , Gao Bin , Yu Shaohua , Chen Lili , Hao Xiaoyi , Liu Han , Lin Zheng 
Published: January 2022

Background: Habituation is considered to have protective and filtering mechanisms. The present study is aim to find the casual relationship and mechanisms of excitatory–inhibitory (E/I) dysfunctions in schizophrenia (SCZ) via habituation.Methods: A dichotic listening paradigm was performed with simultaneous EEG recording on 22 schizophrenia patients and 22 gender- and age-matched healthy controls. Source reconstruction and dynamic causal modeling (DCM) analysis were performed to estimate the effective connectivity and casual relationship between frontal and temporal regions before and after habituation.Results: The schizophrenia patients expressed later habituation onset (p &lt; 0.01) and hyper-activity in both lateral frontal–temporal cortices than controls (p = 0.001). The patients also showed decreased top-down and bottom-up connectivity in bilateral frontal–temporal regions (p &lt; 0.01). The contralateral frontal–frontal and temporal–temporal connectivity showed a left to right decreasing (p &lt; 0.01) and right to left strengthening (p &lt; 0.01).Conclusions: The results give causal evidence for E/I imbalance in schizophrenia during dichotic auditory processing. The altered effective connectivity in frontal–temporal circuit could represent the trait bio-marker of schizophrenia with auditory hallucinations.
Patients' Health & Well-Being in Inpatient Mental Health-Care Facilities: A Systematic Review
Authors: Weber Clara , Monero Flores Virna , Wheele Theresa Poppy , Miedema Elke , White Emma Victoria 
Published: January 2022

Background: Previous research indicates that the physical environment of healthcare facilities plays an important role in the health, well-being, and recovery outcomes of patients. However, prior works on mental healthcare facilities have incorporated physical environment effects from general healthcare settings and patient groups, which cannot be readily transferred to mental healthcare settings or its patients. There appears to be a specific need for evidence synthesis of physical environmental effects in mental healthcare settings by psychopathology.Purpose: This review evaluates the state (in terms of extent, nature and quality) of the current empirical evidence of physical environmental on mental health, well-being, and recovery outcomes in mental healthcare inpatients by psychopathology.Method: A systematic review (PRISMA guidelines) was performed of studies published in English, German, Dutch, Swedish, and Spanish, of all available years until September 2020, searched in Cochrane, Ovid Index, PsycINFO, PubMed, and Web of Science and identified through extensive hand-picking. Inclusion criteria were: Adult patients being treated for mental ill-health (common mental health and mood disorders, Cochrane frame); inpatient mental health care facilities; specifications of the physical and socio-physical environment (e.g., design features, ambient conditions, privacy); all types of empirical study designs. Quality assessment and data synthesis were undertaken.Results: The search retrieved 1,068 titles of which 26 met the inclusion criteria. Findings suggest that there is only indicative evidence of the impact of the physical healthcare environment on patients' mental health, well-being, and recovery outcomes. There is significant lack of pathology-specific evidence. Methodological shortcomings and empirical scarcity account for the poor evidence.Conclusion: This review highlights the need for more research using advanced study designs.
Altered Topological Organization of Functional Brain Networks in Betel Quid Dependence: A Resting-State Functional MRI Study
Authors: Liu Tao , Liu Liting , Chen Hui Juan , Fu Qingqing , Fu Lili , Huang Weiyuan , Chen Feng 
Published: January 2022

Background: Betel quid dependence (BQD) is associated with abnormalities in the widespread inter-regional functional connectivity of the brain. However, no studies focused on the abnormalities in the topological organization of brain functional networks in chewers in Mainland China.Methods: In the current study, resting-state functional magnetic resonance images were acquired from 53 BQD individuals and 37 gender- and age-matched healthy controls (HCs). A functional network was constructed by calculating the Pearson correlation coefficients among 90 subregions in the human Brainnetome Atlas. The topological parameters were compared between BQD individuals and HCs.Results: The results showed that BQD individuals presented a small-world topology, but the normalized characteristic path length (λ) increased compared with HCs (0.563 ± 0.030 vs. 0.550 ± 0.027). Compared to HCs, BQ chewers showed increased betweenness centrality (Be) in the right supplementary motor area, right medial superior frontal gyrus, right paracentral lobule, right insula, left posterior cingulate gyrus, right hippocampus, right post-central gyrus, right superior parietal gyrus, and right supramarginal gyrus, while decreased Be was found in the orbitofrontal area and temporal area, which is associated with reward network, cognitive system, and default mode network. The area under the curve (AUC) value of λ displayed a positive correlation with the duration of BQ chewing (r = 0.410, p = 0.002).Conclusions: The present study revealed the disruption of functional connectome in brain areas of BQD individuals. The findings may improve our understanding of the neural mechanism of BQD from a brain functional network topological organization perspective.
Attempted Suicide in the Older Adults: A Case Series From the Psychiatry Ward of the University Hospital Maggiore Della Carità, Novara, Italy
Authors: Gramaglia Carla , Martelli Maria , Scotti Lorenza , Bestagini Lucia , Gambaro Eleonora , Romero Marco , Zeppegno Patrizia 
Published: January 2022

Introduction: As suicide rates increase with age, it is mandatory to carefully assess old age suicidal behaviors. Our aim was to describe the main socio-demographic and clinical features of a sample of suicide attempters aged 65 years and older, and to assess differences within the sample (men vs. women; patients with vs. without a previous history of suicide attempt; patients with vs. without a previous psychiatric history).Methods: Retrospective study conducted at the Maggiore della Carità University Hospital, Novara, Italy.Results: A higher percentage of female patients in our sample were treated by or referred to mental health services, while a greater percentage of male patients required a prolonged clinical observation in the Emergency Room (ER) or in non-psychiatric wards before psychiatric admission. The percentage of patients without previous psychiatric history taking anxiolytic and sedative medications was 25%.Conclusion: It is likely that different clusters and types of suicide attempters exist. Women in our sample appeared more proactive in asking for help, and more likely to be already treated by or referred to a psychiatric service, suggesting the need to facilitate the access to psychiatric services for the male population aged 65 years and older, or to offer support and care for the non-psychiatric reasons (comorbidities, pain, and loss of autonomy) possibly underlying suicidal behavior in this specific group. The use of medications deserves more attention considering the possible critical diagnostic issues in this age group.