bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Genetic Polymorphism of TNF-α Associated with the Anti-TNF-α Therapy Used for COVID-19 Patients – Another Possible Approach
Authors: Silva Luciano Barreto , Sampaio Guilherme Marinho , Cunha Rodolfo Scavuzzi Carneiro , Muniz Caroline Isabele Félix , Santos Neto Alexandrino Pereira , Anjos Hemanuelly Albuquerque dos , Fonseca Felipe Leonardo de Melo Almeida , Ataíde Filho Ailton Coelho de 
Published: January 2022

Objective: This work aimed of to review the implications of anti-TNF-α therapy in COVID-19 patients associated with the genetic polymorphism (TNF-α polymorphisms in the region-308) of this pertinent cytokine Methodology: Electronic searches were carried out on PUBMED Central, BVS/BIREME, Web of Science and The Cochrane Library with the aid of key-words. Results: Twenty-six articles were collected. Anti-TNF-α therapy was interpreted and evaluated. Conclusions: Although scarce information is available in the current literature, anti- TNF-α therapy seems to be a viable clinical approach for hospitalized COVID-19 patients who do not need oxygen supply. The genetic polymorphisms, although relevant, may be useful for further researched to assess the clinical response in different research groups.
Leite cru refrigerado: qualidade microbiológica, físico-química e detecção de resíduos de antibióticos
Authors: Ulisses Alessandra de Fátima , Píccolo Maria da Penha , Rangel Otacílio José Passos , Santos Júnior Alexandro Cristiano , Maia Júnior Jonhny de Azevedo 
Published: January 2022

Objetivou-se avaliar a qualidade microbiológica, físico-química além da verificação da presença de resíduos de antibióticos em amostras de leite cru refrigerado coletadas em quatro tanques individuais e cinco coletivos localizados em propriedades rurais pertencentes ao município de Alegre/ES. As amostras foram codificadas, acondicionadas em frascos esterilizados e transportadas ao Laboratório de Microbiologia e Biotecnologia do Instituto Federal do Espírito Santo Ifes, Campus de Alegre/ES. Realizou-se análises microbiológicas para contagem bacteriana total e Staphylococcus aureus; físico-químicas; além da detecção de resíduos de antibióticos dos grupos β-lactâmicos e tetraciclinas. Os valores médios para CBT ficaram entre 2,0 x 104 UFC mL-1 e 3,0 x 107 UFC mL-1 e constatou-se que 44,44% das amostras encontravam-se em conformidade com a legislação. Os valores de S. aureus ficaram entre <102 e 3,6x106 UFC mL-1 e estes resultados são preocupantes devido à possibilidade de formação de toxinas termorresistentes, além de ser um microrganismo causador de mastite. Em relação às análises físico-químicas, 33,33% encontraram-se em conformidade com a legislação e em todas as amostras não foram constatados resíduos de antibióticos. Concluiu-se que apenas as amostras coletadas nos tanques de refrigeração individuais A6 e A7, se encontraram de acordo com a legislação vigente. São necessárias mudanças nas práticas de manejo adotadas para obtenção de leite com qualidade e inocuidade. A adoção dessas medidas permitiria a obtenção de leite com qualidade e inocuidade além de contribuir para a saúde dos consumidores e para a sustentabilidade do setor na região e no estado do Espírito Santo.
Atuação do enfermeiro em situações de desastres naturais: uma revisão integrativa
Authors: Barboza Laine da Costa Almeida , Melo Karine Costa , Silva Marconny Lira da , Costa Jaqueline Brito da , Mendes Rogério Cruz , Silva Derik Mikkel de Sousa , Marques Victoria Ribeiro de Sousa , Santos Paulo Sérgio Gaspar dos , Hernandes Linccon Fricks , Costa Ana Carla Marques da , Santos Márcia Sousa , Viana Camilla Lohanny Azevedo , Siqueira Francisléia Falcão França Santos , Cunha Hádila Giovanna Santos Siqueira , Teixeira Solygardia Albuquerque Maciel , Lemos Ana Valéria Lopes 
Published: January 2022

Os desastres podem acontecer por diversos fenômenos naturais, as vezes pela junção de dois ou mais, sendo classificados em tecnológicos ou naturais. Estes eventos adversos geralmente atingem pessoas que já se encontram em situação de vulnerabilidade, podendo estar relacionada diretamente a transtornos psicológicos, podendo ainda, afetar os profissionais. O trabalho teve como objetivo geral analisar com base na literatura científica a atuação do enfermeiro em situações de desastres naturais. Trata-se de uma pesquisa bibliográfica do tipo Revisão Integrativa (RI) da literatura, que utilizou os Descritores em Ciências da Saúde (DeCS) e dos títulos da Cumulative Index to Nursing and Allied Health Literature (CINAHL) e Mesh terms. Ao todo foram selecionados onze estudos, a maior parte apresentou abordagem qualitativa dos dados, correspondendo a sete (63,3%) trabalhos, em que a base de dados da National Library of Medicine apresentou o maior número de estudos (45,4%). A maior parte das publicações concentraram-se no ano de 2019 (36,3%) e 2020 (27,2%). Quanto ao idioma dez (90%) das publicações estavam na língua inglesa; em que o Brasil foi o país com o maior número de publicações (27,2%). A assistência prestada aos pacientes vítimas de desastres naturais, deve ser focada em aspectos físicos e psicológicos. Nesse caso, a enfermagem deve entrar em ação lidando diretamente com o sujeito e prestando esclarecimentos acerca do procedimento realizado por ele, além de realizar escuta ativa, quando o paciente tem condições e deseja verbalizar, oferecer conforto e utilizar o toque como fonte de segurança aos clientes.
Perfil de pacientes em hemodiálise de um serviço de referência do interior do Rio Grande do Sul, Brasil
Authors: Souza Marri Andressa Hilbert de , Holanda Rubellita , Martins Edna Thaís Jeremias , Hoffmeister Andriele Cristina , Capellari Claudia 
Published: January 2022

Objetivo: Identificar o perfil sociodemográfico e clínico de pacientes com Doença Renal Crônica em hemodiálise em um serviço de referência no interior do Rio Grande do Sul, RS. Metodologia: Estudo quantitativo, transversal e com característica de censo. Participaram 94 pacientes com Doença Renal Crônica (DRC), em tratamento hemodialítico, vinculados a um serviço de nefrologia do interior do Rio Grande do Sul, Brasil. A coleta de dados ocorreu entre setembro e outubro de 2020, por meio de consulta de enfermagem, exames de rotina, prontuários e questionários estruturados. Resultados: 94 pacientes foram incluídos no estudo, com idade média de 53,1(±13,7) anos, prevalência do sexo masculino (61,7%), casados (45,7%), renda de 1 a 2 salários mínimos (46,8%), escolaridade com fundamental incompleto (62,8%), cor branca (80,9%). Quanto aos aspectos clínicos, a principal etiologia da DRC foi a hipertensão (43,6%), o tempo de diagnóstico foi em média 6,05 (±6,46) anos, acesso venoso por fístula arteriovenosa para hemodiálise (94,7%). As principais complicações foram hipotensão (56,4%) e câimbras (28,7%). Conclusão: Na amostra, prevaleceram homens brancos, casados e de baixa escolaridade, com hipertensão arterial como principal fator etiológico. A variabilidade dos achados aponta para a necessidade de individualização do cuidado a essa população.
Manobras de desobstrução e acesso as vias aéreas nas emergências odontológicas
Authors: Lima Lohana Maylane Aquino Correia de , Lima Mayana Aquino Correia de , Lima Rayana Cruz Correia de , Pinheiro Milena Mello Varela Ayres de Melo , Melo Rodrigo Henrique Mello Varela Ayres de , Rhoden Deise Louise Bohn , Melo Victor Leonardo Mello Varela Ayres de , Melo Junior Frederico Marcio Varela Ayres de , Melo Bruna Heloísa Costa Varela Ayres de , Beck Júlia de Souza , Lins Maria Luísa Alves , Melo Jussara Diana Varela Ayres de , Freitas Nely Dulce Varela de Melo Costa , Bustamante Neme Portal , Melo Ricardo Eugenio Varela Ayres de 
Published: January 2022

A via aérea humana é um sistema composto de importantes estruturas anatômicas que protegem e mantêm a permeabilidade. Este trabalho objetiva apresentar as manobras de desobstrução e acesso às vias aéreas em situações de emergência. Realizou-se uma revisão de literatura com busca nas bases de dados Scielo e Embase. Para isto, utilizou-se descritores "Airway Obstruction" e "Emergency" e “Dentistry” com 40 artigos triados. Nas manobras básicas, a manobra de Heimlich consiste na compressão infradiafragmática em “L”. A manobra de Jaw Thrust é caracterizada pela protrusão da mandíbula, enquanto que Chin Lift realiza-se a hiperextensão cervical e elevação mentual. Nas manobras avançadas a Intubação Endotraqueal garante o fluxo do ar nas vias aéreas por meio da aposição de uma cânula por via oral ou nasal, a Cricotireoidostomia é um procedimento invasivo que cria uma abertura através da membrana cricotireoidea, enquanto que a Traqueostomia consiste em um procedimento cirúrgico para a criação de uma abertura entre o 2⁰ e o 3⁰ anel traqueal. Por isso, a manobra de Heimlich é eleita como primeira alternativa nas situações de obstrução aguda seguida das manobras de Jaw Thrust, Chin Lift e a Intubação Endotraqueal. A Cricotireoidostomia é o procedimento invasivo de primeira escolha para o reestabelecimento da ventilação em situações de emergência, enquanto que a Traqueostomia, de caráter eletivo, demanda maior tempo de procedimento. Portanto, a obstrução aguda das vias aéreas superiores é uma complicação grave e de caráter emergencial, por isso deve o Cirurgião-Dentista estar preparado para reverter esse quadro rapidamente nessas situações.
DINAMIKA PROSES REKRUTMEN KOMISIONER KPU PROVINSI SULAWESI BARAT TAHUN 2018
Authors: Asnur Ros Pratiwi , Yakub Andi , Kambo Gustiana A 
Published: January 2022

Penelitian ini bertujuan untuk mengkaji dan menganalisis dinamika proses rekrutmen komisioner KPU Provinsi Sulawesi Barat pada tahun 2018. Tipe penelitian yang digunakan dalam penelitian ini adalah kualitatif yang memberikan gambaran proses rekrutmen komisioner KPU Provinsi Sulawesi Barat pada tahun 2018. Hasil penelitian ini menunjukkan bahwa dalam melaksanakan proses rekrutmen calon anggota komisioner yang melibatkan tim seleksi dalam melaksanakan semua tahapan mulai dari pendaftaran, penelitian administrasi, tes tertulis/CAT, tes psikologi, tes kesehatan, wawancara, diperlukan sistem rekrutmen yang ketat, transparan, dan berintegritas dengan tujuan untuk menghasilkan anggota komisioner yang berkompeten dan kredibel. Tim seleksi mempunyai peran penting dalam proses ini, mereka dituntut untuk senantiasa bersikap independen untuk menghindari tuduhan-tuduhan dari berbagai pihak sehingga tidak ada kesan bahwa Timsel berpihak kepada salah satu calon. Selain itu, sikap prudence juga seharusnya selalu menjadi perhatian bagi para Tim seleksi terutama dalam hal pemeriksaan berkas sebagai kelengkapan yang harus dipenuhi oleh para calon komisioner, tentu hal ini dimaksudkan untuk meminimalisir terjadinya pelanggaran-pelanggaran administrasi yang berpotensi terhadap timbulnya gugatan atas legalitas keputusan yang ditetapkan oleh Tim Seleksi.
Kekuatan Politik Pemangku Adat Ammatoa Pada Masyarakat Adat di Tanatoa Bulukumba
Authors: Kambo Gustiana A 
Published: January 2022

Penelitian ini bertujuan untuk mendeskripsikan Eksistensi Komunitas Adat Ammatoa dan legitimasi kekuatan Politik Ammatoa. Penelitian ini bersifat deskriptif–analisis, penentuan informan dilakukan secara purposive dan pengumpulan data dilakukan melalui wawancara dan kajian pustaka. Data dikembangkan menjelaskan, menguraikan dan menganalisis secara rinci budaya dan adat istiadat berdasarkan tujuan penelitian. Hasil penelitian menggambarkan pemangku adat Ammatoa yang memiliki kekuatan politik dalam menjalankan fungsinya sebagai pemimpin adat pada masyarakat adat di Tanatoa Bulukumba. Ammatoa bertindak sebagai pemimpin informal yang juga berfungsi sebagai tokoh masyarakat yang memiliki kharisma yang memiliki nilai lebih dibanding yang lainnya. Pada setiap pemilihan umum, Ammatoa memiliki pengaruh besar, para calon yang akan ikut dalam konstalasi tersebut mendatangi Ammatoa untuk meminta restu dan berharap pengaruhnya untuk mendapat dukungannnya di Tanatoa bersama masyarakat adatnya.
Hemodynamic Assessment in Bicuspid Aortic Valve Disease and Aortic Dilation: New Insights From Voxel-By-Voxel Analysis of Reverse Flow, Stasis, and Energetics
Authors: Geeraert Patrick , Jamalidinan Fatemehsadat , Burns Fiona , Jarvis Kelly , Bristow Michael S. , Lydell Carmen , Hidalgo Tobon Silvia S. , de Celis Alonso Benito , Fedak Paul W. M. , White James A. , Garcia Julio 
Published: January 2022

Objectives: Clinical management decisions surrounding ascending aorta (AAo) dilation in bicuspid aortic valve (BAV) disease benefit from personalized predictive tools. 4D-flow MRI may provide patient-specific markers reflective of BAV-associated aortopathy. This study aims to explore novel 4D-flow MRI parametric voxel-by-voxel forward flow, reverse flow, kinetic energy and stasis in BAV disease. We hypothesize that novel parametric voxel-by-voxel markers will be associated with aortic dilation and referral for surgery and can enhance our understanding of BAV hemodynamics beyond standard metrics.Methods: A total of 96 subjects (73 BAV patients, 23 healthy controls) underwent MRI scan. Healthy controls had no known cardiovascular disease. Patients were clinically referred for AAo dilation assessment. Indexed diameters were obtained by dividing the aortic diameter by the patient’s body surface area. Patients were followed for the occurrence of aortic surgery. 4D-flow analysis was performed by a single observer in five regions: left ventricular outflow tract (LVOT), AAo, arch, proximal descending aorta (PDAo), and distal descending aorta (DDAo). In each region peak velocity, kinetic energy (KE), forward flow (FF), reverse flow (RF), and stasis were measured on a voxel-by-voxel basis. T-tests (or non-parametric equivalent) compared flow parameters between cohorts. Univariate and multivariate analyses explored associations between diameter and parametric voxel-by-voxel parameters.Results: Compared to controls, BAV patients showed reduced stasis (p &lt; 0.01) and increased RF and FF (p &lt; 0.01) throughout the aorta, and KE remained similar. In the AAo, indexed diameter correlated with age (R = 0.326, p = 0.01), FF (R = −0.648, p &lt; 0.001), RF (R = −0.441, p &lt; 0.001), and stasis (R = −0.288, p &lt; 0.05). In multivariate analysis, FF showed a significant inverse association with AAo indexed diameter, independent of age. During a median 179 ± 180 days of follow-up, 23 patients (32%) required aortic surgery. Compared to patients not requiring surgery, they showed increased KE and peak velocity in the proximal aorta (p &lt; 0.01), accompanied by increased RF and reduced stasis throughout the entire aorta (p &lt; 0.01).Conclusion: Novel voxel-by-voxel reverse flow and stasis were altered in BAV patients and are associated with aortic dilation and surgical treatment.
Immobilization of β-Galactosidase by Encapsulation of Enzyme-Conjugated Polymer Nanoparticles Inside Hydrogel Microparticles
Authors: Suvarli Narmin , Wenger Lukas , Serra Christophe , Perner-Nochta Iris , Hubbuch Jürgen , Wörner Michael 
Published: January 2022

Increasing the shelf life of enzymes and making them reusable is a prominent topic in biotechnology. The encapsulation inside hydrogel microparticles (HMPs) can enhance the enzyme’s stability by preserving its native conformation and facilitating continuous biocatalytic processes and enzyme recovery. In this study, we present a method to immobilize β-galactosidase by, first, conjugating the enzyme onto the surface of polymer nanoparticles, and then encapsulating these enzyme-conjugated nanoparticles (ENPs) inside HMPs using microfluidic device paired with UV-LEDs. Polymer nanoparticles act as anchors for enzyme molecules, potentially preventing their leaching through the hydrogel network especially during swelling. The affinity binding (through streptavidin-biotin interaction) was used as an immobilization technique of β-galactosidase on the surface of polymer nanoparticles. The hydrogel microparticles of roughly 400 μm in size (swollen state) containing unbound enzyme and ENPs were produced. The effects of encapsulation and storage in different conditions were evaluated. It was discovered that the encapsulation in acrylamide (AcAm) microparticles caused an almost complete loss of enzymatic activity. Encapsulation in poly(ethylene glycol) (PEG)-diacrylate microparticles, on the other hand, showed a residual activity of 15–25%, presumably due to a protective effect of PEG during polymerization. One of the major factors that affected the enzyme activity was presence of photoinitiator exposed to UV-irradiation. Storage studies were carried out at room temperature, in the fridge and in the freezer throughout 1, 7 and 28 days. The polymer nanoparticles showcased excellent immobilization properties and preserved the activity of the conjugated enzyme at room temperature (115% residual activity after 28 days), while a slight decrease was observed for the unbound enzyme (94% after 28 days). Similar trends were observed for encapsulated ENPs and unbound enzyme. Nevertheless, storage at −26°C resulted in an almost complete loss of enzymatic activity for all samples.
Design of a Controlled-Release Delivery Composite of Antibacterial Agent Gatifloxacin by Spherical Silica Nanocarrier
Authors: Guo Xueping , Mo Wenjing , Zhang Dingyang , Wang Yurong , Cao Fang , Zhai Tianyun , Rao Wenhua , Guan Xiong , Xu Lei , Pan Xiaohong 
Published: January 2022

In this study, a spherical silica nanoparticle was explored as a gatifloxacin carrier synthesized by the chemical precipitation method. It was found that there was no new chemical bond formation during the loading process between gatifloxacin and silica, which implies that the binding was driven by physical interaction. In addition, the drug loading and encapsulation efficiency could be improved by appropriately increasing nano-silica content in the loading process. Meanwhile, the release rate of gatifloxacin after loading nano-silica was also improved, suggesting the successful design of a controlled-release delivery composite. The silica nanocarrier could significantly improve the antibacterial performance of Escherichia coli by 2.1 times, which was higher than the pure gatifloxacin. The 24 h bacteriostatic rate was higher than that of a simple mixture of silica nanoparticles and gatifloxacin. Strong reactive oxygen species (ROS) in GAT-SiO2 NPs suggests that ROS might be associated with bactericidal activity. The synergy between the physicochemical effect and ROS production of this material is proposed as the mechanism of its antibacterial activity, which can also be confirmed by the cell membrane damage observed under electron microscopy and DNA damage experiments. Collectively, our finding indicates that nano-silica microspheres could serve as a promising carrier for the sustained release of gatifloxacin, thereby providing a new carrier design scheme for the improvement of the antibacterial effect.
Clinical Characteristics of Cryoglobulinemia With Cardiac Involvement in a Single Center
Authors: He Kun , Zhang Yun , Wang Wei , Wang Yu , Sha Yue , Zeng Xuejun 
Published: January 2022

Background: Cryoglobulinemia is a syndrome characterized by the presence of cryoglobulins (CGs) in serum, and cardiac involvement is a rare occurrence that can affect treatment and prognosis. This study aimed to explore the clinical characteristics of cryoglobulinemia with cardiac involvement.Methods: 108 patients diagnosed with cryoglobulinemia who were admitted and treated in Peking Union Medical College Hospital (PUMCH) between June 1985 and June 2019 were enrolled in the present study. Clinical characteristics, therapy, and prognosis of patients with cardiac involvement were retrospectively analyzed.Results: The cryoglobulinemia with cardiac involvement was found in 7 patients, thus reaching the incidence of 6.5%. Heart failure was the main cardiac manifestation found in these patients, all with the involvement of external cardiac organs. Laboratory examinations showed significant elevation of N-terminal brain natriuretic peptide precursor (NT-proBNP) and brain natriuretic peptide (BNP) with negative troponin (cTnI). Electrocardiogram (ECG) was generally normal or only showed low-flat and biphasic multi-lead T waves. Echocardiography was performed in 6 patients, all of whom showed enlargement of heart cavity. Five patients had reduced left ventricular myocardial contractible motion with decreased ejection fraction, 3 patients had pericardial effusion, and 1 patient had left ventricular hypertrophy or severe aortic insufficiency. Cardiac magnetic resonance imaging showed delayed myocardial enhancement in 2 patients. One patient underwent a myocardial biopsy, which showed perivasculitis. Condition in 6 patients who received active treatment targeting improved in the early stage. Three patients (3/7, 42.9%) died due to disease progression during follow-up period.Conclusions: Cryoglobulinemia with cardiac involvement is a rare but serious condition that has relatively high risk of death. When patients with cryoglobulinemia without underlying heart disease experience heart failure, chest pain, or elevation of asymptomatic NT-proBNP and BNP, there is a high possibility of cardiac involvement, even if the electrocardiogram and troponin are negative. Further examinations such as echocardiography, cardiac magnetic resonance imaging, and myocardial biopsy examination could contribute to the diagnosis. Cardiac manifestations could be timely reversed after active targeted treatment. NT-proBNP and echocardiography could be used for the monitoring of disease efficacy.
Trpc6 Promotes Doxorubicin-Induced Cardiomyopathy in Male Mice With Pleiotropic Differences Between Males and Females
Authors: Norton Nadine , Bruno Katelyn A. , Di Florio Damian N. , Whelan Emily R. , Hill Anneliese R. , Morales-Lara Andrea Carolina , Mease Anna A. , Sousou John M. , Malavet Jose A. , Dorn Lauren E. , Salomon Gary R. , Macomb Logan P. , Khatib Sami , Anastasiadis Zacharias P. , Necela Brian M. , McGuire Molly M. , Giresi Presley G. , Kotha Archana , Beetler Danielle J. , Weil Raegan M. , Landolfo Carolyn K. , Fairweather DeLisa 
Published: January 2022

Background: Doxorubicin is a widely used and effective chemotherapy, but the major limiting side effect is cardiomyopathy which in some patients leads to congestive heart failure. Genetic variants in TRPC6 have been associated with the development of doxorubicin-induced cardiotoxicity, suggesting that TRPC6 may be a therapeutic target for cardioprotection in cancer patients.Methods: Assessment of Trpc6 deficiency to prevent doxorubicin-induced cardiac damage and function was conducted in male and female B6.129 and Trpc6 knock-out mice. Mice were treated with doxorubicin intraperitoneally every other day for a total of 6 injections (4 mg/kg/dose, cumulative dose 24 mg/kg). Cardiac damage was measured in heart sections by quantification of vacuolation and fibrosis, and in heart tissue by gene expression of Tnni3 and Myh7. Cardiac function was determined by echocardiography.Results: When treated with doxorubicin, male Trpc6-deficient mice showed improvement in markers of cardiac damage with significantly reduced vacuolation, fibrosis and Myh7 expression and increased Tnni3 expression in the heart compared to wild-type controls. Similarly, male Trpc6-deficient mice treated with doxorubicin had improved LVEF, fractional shortening, cardiac output and stroke volume. Female mice were less susceptible to doxorubicin-induced cardiac damage and functional changes than males, but Trpc6-deficient females had improved vacuolation with doxorubicin treatment. Sex differences were observed in wild-type and Trpc6-deficient mice in body-weight and expression of Trpc1, Trpc3 and Rcan1 in response to doxorubicin.Conclusions: Trpc6 promotes cardiac damage following treatment with doxorubicin resulting in cardiomyopathy in male mice. Female mice are less susceptible to cardiotoxicity with more robust ability to modulate other Trpc channels and Rcan1 expression.