bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pyrimethamine inhibits cell growth by inducing cell senescence and boosting CD8+ T-cell mediated cytotoxicity in colorectal cancer
Authors: Dong Haiyan , Hu Limei , Li Weiqian , Shi Mengchen , He Lingyuan , Wang Chen , Hu Yijia , Wang Huihui , Wen Chuangyu , Liu Huanliang , Yang Xiangling 
Published: March 2022

Abstract
Background
The emergence of nonresponse or resistance to traditional chemotherapeutic agents is one of the main challenges of colorectal cancer (CRC) therapies. Thus, novel therapeutic drugs that can improve the clinical outcomes of CRC patients are urgently needed. The purpose of this study was to investigate the effects and mechanisms of pyrimethamine in CRC.

Methods and results
In this study, we assessed the role of pyrimethamine on CRC cell growth by cell counting kit-8 and colony formation assays. Cell cycle distribution and cellular senescence were determined by flow cytometry and senescence-associated β-galactosidase staining respectively. RNA-seq analysis and western blotting were used to investigate the potential pathways of pyrimethamine in CRC development. Moreover, animal experiments were performed to evaluate the effect of pyrimethamine in vivo. Our results demonstrated that pyrimethamine could inhibit cell growth by inducing S phase arrest followed by cellular senescence in CRC cells, and the p38MAPK-p53 axis was probably involved in that effect. In addition, pyrimethamine could also boost CD8+ T-cell mediated cytotoxicity and exert antitumor activity in vivo.

Conclusion
These results indicated that pyrimethamine may be a promising candidate agent for CRC treatment.
Design of Distance Assistance System for Intelligent Education by Web-based Applications
Authors: Yang Chao , Lin Jerry Chun-Wei 
Published: March 2022

AbstractIn order to improve the quality of distance education and solve the problem of slow data processing of the teaching system, an intelligent distance education assistance system based on WEB is developed in this paper. After verification, students, teachers and administrators log into the distance intelligent teaching assistance system and transmit various information to the interface of the teaching and administration subsystems. The submitted information is merged using the Bayesian model for integrating educational resources in the digital cloud to create a distance education database that supports the system with data. At the same time, the evaluation of the business logic of the data is performed. After the data is converted to other formats in the subsequent convertible database, it returns to the user interface to provide browsing and consulting functions for users. The experimental results show that the designed system can realize the remote auxiliary function of intelligent education and effectively improve the quality of teaching. The real-time data acquisition rate of the system in this paper is always equal to the set value. The average acceleration of the system in this paper is 5.5 and the data processing efficiency is higher. The minimum safety factor of the system is between 7.8 and 8.5, and the system has high stability. The user satisfaction of the system is over 93%, and the accuracy of the collected data is relatively high. The auxiliary system designed in this paper can provide a stable and efficient application environment for distance education.
Towards a better understanding of the role of stabilizers in QESD crystallizations
Authors: Hansen Jerome , Kleinebudde Peter 
Published: March 2022

AbstractQuasi-emulsion solvent-diffusion crystallization (QESD) is a type of spherical crystallization which can be used as a particle design method to improve the flowability and micromeritic properties of drugs or excipients. Spherical particles are generated by dispersing a solvent phase in an antisolvent so that a transient emulsion is formed. Within the droplets the material can crystallize and agglomerate into spherical, hollow particles. Surfactants, such as surface-active polymers like hypromellose, are often required to stabilize the quasi-emulsion. To gain further understanding for the role of the stabilizer, a new screening-method was developed which compared different surface active polymers in solution at similar dynamic viscosities rather than at a set concentration. The dynamic viscosities of a low-viscosity grade hypromellose solution used in the previous publications describing the QESD crystallization of metformin hydrochloride by the authors was used as a target value. QESD crystallizations of metformin hydrochloride (MF) and celecoxib showed that the type of stabilizer and whether it is dissolved in the solvent or antisolvent has an effect on the agglomerates. For MF, the type of hypromellose used can have a significant influence on the properties of the agglomerates. More polymers could be used to stabilize the transient emulsion of celecoxib than previously found in literature. Furthermore, QESD crystallizations seem to be more robust when the stabilizer is dissolved in the antisolvent, however this can lead to a reduced drug load of the agglomerates.
Geochemical assessment of groundwater in semiarid area, case study of the multilayer aquifer in Djelfa, Algeria
Authors: Ali Rahmani Salah Eddine , Chibane Brahim 
Published: March 2022

AbstractThe best understanding of the geochemical process of groundwater is important for a sustainable development and for a strategic management of water resources. A hydro-geochemical study of groundwater of the Djelfa multilayer aquifers systems was established, using stable isotopes, and major elements, in order to identify the groundwater mineralization process occurring in this system. The chemical data indicate that the dissolution of evaporate minerals (Gypsum, halite, anhydrite) and the evaporation from water table are the principle processes who command the groundwater mineralization. Groundwater samples are depleted in stables isotopes (18O and 2H) reflecting a slug of water recharged during colder climatic conditions, and the recharge was occurred at different altitude. The analysis of the replenishment of aquifer levels shows that the level arises in the replenishment of aquifer and decreases in the depletion of aquifer due to the phenomena of evaporation and to the extensive pumping from wells. Excessive pumping creates a remarkable cone of depression that makes flow direction from syncline to the basin outlet.
Greater Than the Sum of its Parts: Computationally Flexible Bayesian Hierarchical Modeling
Authors: Johnson Devin S. , Brost Brian M. , Hooten Mevin B. 
Published: March 2022

AbstractWe propose a multistage method for making inference at all levels of a Bayesian hierarchical model (BHM) using natural data partitions to increase efficiency by allowing computations to take place in parallel form using software that is most appropriate for each data partition. The full hierarchical model is then approximated by the product of independent normal distributions for the data component of the model. In the second stage, the Bayesian maximuma posteriori(MAP) estimator is found by maximizing the approximated posterior density with respect to the parameters. If the parameters of the model can be represented as normally distributed random effects, then the second-stage optimization is equivalent to fitting a multivariate normal linear mixed model. We consider a third stage that updates the estimates of distinct parameters for each data partition based on the results of the second stage. The method is demonstrated with two ecological data sets and models, a generalized linear mixed effects model (GLMM) and an integrated population model (IPM). The multistage results were compared to estimates from models fit in single stages to the entire data set. In both cases, multistage results were very similar to a full MCMC analysis. Supplementary materials accompanying this paper appear online.
Narrative inquiry for CALL teacher preparation programs amidst the COVID-19 pandemic: language teachers’ technological needs and suggestions
Authors: Tafazoli Dara , Meihami Hussein 
Published: March 2022

AbstractDrawing on qualitative research, this study explores the Iranian EFL teachers’ technological needs and their suggestions for using computer-assisted language learning (CALL) during the COVID-19 pandemic. We analyzed the narratives produced by 66 English as a Foreign Language (EFL) teachers for their themes through deductive and inductive thematic analysis phases using MAXQDA. The findings indicate that CALL teacher preparation programs should prioritize technological pedagogical knowledge (TPK), then technological content knowledge (TCK), and finally technological knowledge (TK). Moreover, teachers stated that CALL teacher preparation programs should develop their knowledge concerning the intersections of TCK/TPK and TPK/TK. Furthermore, Iranian EFL teachers suggested that CALL can be used during the pandemic if cooperation among teachers, students, and parents will be made, technological-related infrastructures will be developed, and teacher preparation programs will develop EFL teachers’ collaboration, digital literacy, teacher autonomy, and TPK with special attention to the educational needs made by the pandemic situation. The findings have implications for teacher educators, professional development course designers and providers, and decision-makers by highlighting promising directions to devote their precious time and resources.
Development of a transdiagnostic stepped care programme for common adolescent mental health problems in Indian secondary schools: lessons from a pilot study examining acceptability and feasibility
Authors: Malik Kanika , Ibrahim Maliha , Mathur Sonal , Jose James E. , Nair Pooja , Sahu Rooplata , Krishna Madhuri , Jangra Deepak , Mathews Rhea , Cuijpers Pim , Chorpita Bruce , Fairburn Christopher G. , Patel Vikram , Michelson Daniel 
Published: March 2022

AbstractBackgroundThe ‘PRemIum for aDolEscents’ (PRIDE) project has developed a school-based, transdiagnostic stepped care programme for common adolescent mental health problems in India. The programme comprises a brief problem-solving intervention (‘Step 1’) followed by a personalised cognitive-behavioural intervention (‘Step 2’) for participants who do not respond to the first step.MethodsA mixed-method design was used to evaluate the acceptability and feasibility of the stepped care programme in five schools in New Delhi. Participants were N = 80 adolescents (mean age = 15.3 years, females = 55%) with elevated mental symptoms and associated distress/impairment.Results61 (76%) of the enrolled sample were assessed following Step 1, from which 33 (54%) met non-remission criteria. Among these 33 non-remitted cases, 12 (36%) opted for Step 2 and five (42%) completed the full programme. The remaining non-remitted cases (n = 21, 64%) opted out of further treatment. Perceived resolution of the primary problem (n = 9, 43%) was the most common reason for opting out. The median time to complete each step was 22 and 70 days respectively, with a gap of 31 days between steps. Qualitative feedback from adolescents and counsellors indicated requirements for a shorter delivery schedule, greater continuity across steps and more collaborative decision-making.ConclusionsThis study provides preliminary evidence for a stepped care programme aimed at common adolescent mental health problems. Modifications are recommended to enhance the acceptability and feasibility of the programme in low-resource settings.
Work incapacity and psychiatric patient care following attempted suicide – a cohort study of 65 097 Swedish twins
Authors: Lindberg Anton , Svedberg Pia , Ropponen Annina , Narusyte Jurgita , Wang Mo 
Published: March 2022

AbstractBackgroundResearch is scarce on the role of familial factors and previous psychiatric care on the association between suicide attempt and future work incapacity as well as deterioration in mental health. We aimed to investigate the associations between suicide attempt and sickness absence, disability pension and psychiatric patient care and to study the influence of previous psychiatric care and familial factors (genetics and shared environment) on the associations.MethodsThe study included 65 097 twins living in Sweden on 31st of December 2006, aged 19–60 years. The twins were followed 2007–2013 regarding sickness absence, disability pension, inpatient care or specialized outpatient care for a mental diagnosis. Cox regression models were performed for the whole sample, and conditional models for discordant twin pairs. The analyses were also stratified by psychiatric care before 2007.ResultsWe found that suicide attempt predicted sickness absence, disability pension, and future mental diagnosis among the whole sample. The discordant twin pair analyses showed that the association between suicide attempt and sickness absence or disability pension was influenced by familial factors. Stratified analyses of individuals with or without psychiatric care before 2007 showed that previous psychiatric care had some impact on the associations.ConclusionsA suicide attempt is a risk factor for work incapacity and psychiatric patient care. Familial factors and previous psychiatric care play a role in the associations between attempting suicide and work incapacity as well as psychiatric patient care. These factors are important when developing measures preventing work incapacity among those with a suicide attempt.
The gender pension gap in Germany: is divorce a gender-equaliser?
Authors: Kreyenfeld Michaela , Schmauk Sarah , Mika Tatjana 
Published: March 2022

AbstractGermany is one of the few countries in Europe that has implemented a system of ‘divorce splitting’. Under this system, the pension credits that spouses have accumulated during their marriage are combined and then split equally between them upon divorce. This study examines how divorce affects public pension entitlements in Germany, and how these patterns are influenced by the system of divorce splitting. The data for our analysis comes from SHARE-RV, a direct linkage of the Survey of Health, Ageing and Retirement in Europe (SHARE) with administrative data of the research data centre of the German Pension Insurance Fund (Deutsche Rentenversicherung Bund). The data include information on the beneficiaries’ monthly earnings and employment biographies, as well as on their pension entitlements and the credits they received through divorce splitting. The results of the analysis, which was restricted to West German men and women born between 1935 and 1954, reveal that there are large gender gaps in public pension benefit levels. However, the investigation also shows that single and divorced West German women have larger personal pension entitlements than their married or widowed counterparts. Furthermore, the public pension entitlements of divorced men and women in West Germany are very similar. This can be attributed partially to the divorce-splitting system, which tends to increase the pension benefits of divorced women, while reducing the pension benefits of their male counterparts.
Feasibility and measurement error in using food supply data to estimate diet costs in Canada
Authors: Luongo Gabriella , Tarasuk Valerie , Yi Yanqing , Mah Catherine L 
Published: March 2022

AbstractObjective:The cost of food is a key influence on diet. The majority of diet cost studies match intake data from population-based surveys to a single source of food supply prices. Our aim was to examine the methodological significance of using food supply data to price dietary intakes.Methods:Nationally representative 24-h dietary recall data from the 2015 Canadian Community Health Survey-Nutrition (CCHS-N) was matched to the 2015 Canadian Consumer Price Index (CPI) food price list. Proportions and means of reported intakes covered by the 2015 CPI price list were used to compare reported intakes of food groups and food components of interest and concern overall and by quartile of CPI coverage.Setting:Canada.Participants:In total, 20 487 Canadians ages one and older.Results:The CPI covered on average 76·3 % of total dietary intake (g) without water. Staple food groups that were more commonly consumed had better CPI price coverage than those less commonly consumed. Yet some food groups (vegetables, additions and sweets) that were also commonly consumed by Canadians were not well covered by price data. Individuals in the poorest CPI coverage quartile reported consuming significantly greater gram weight (g), dietary fibre (g) and energy (kcal) as compared with those with the best coverage.Conclusions:Differential CPI price coverage exists among food components and commonly consumed food groups; additionally dietary intake differs significantly in the population by CPI coverage. Methodological refinements are needed to better account for error when using prices from food supply data to estimate diet costs.
Accurate and Robust Calibration of the Uniform Affine Transformation Between Scan-Camera Coordinates for Atom-Resolved In-Focus 4D-STEM Datasets
Authors: Ning Shoucong , Xu Wenhui , Ma Yinhang , Loh Leyi , Pennycook Timothy J. , Zhou Wu , Zhang Fucai , Bosman Michel , Pennycook Stephen J. , He Qian , Loh N. Duane 
Published: March 2022

AbstractAccurate geometrical calibration between the scan coordinates and the camera coordinates is critical in four-dimensional scanning transmission electron microscopy (4D-STEM) for both quantitative imaging and ptychographic reconstructions. For atomic-resolved, in-focus 4D-STEM datasets, we propose a hybrid method incorporating two sub-routines, namely a J-matrix method and a Fourier method, which can calibrate the uniform affine transformation between the scan-camera coordinates using raw data, without a priori knowledge of the crystal structure of the specimen. The hybrid method is found robust against scan distortions and residual probe aberrations. It is also effective even when defects are present in the specimen, or the specimen becomes relatively thick. We will demonstrate that a successful geometrical calibration with the hybrid method will lead to a more reliable recovery of both the specimen and the electron probe in a ptychographic reconstruction. We will also show that, although the elimination of local scan position errors still requires an iterative approach, the rate of convergence can be improved, and the residual errors can be further reduced if the hybrid method can be firstly applied for initial calibration. The code is made available as a simple-to-use tool to correct affine transformations of the scan-camera coordinates in 4D-STEM experiments.
Evaluation of In Situ Antiaging Activity in Saccharomyces cerevisiae BY611 Yeast Cells Treated with Polyalthia longifolia Leaf Methanolic Extract (PLME) Using Different Microscopic Approaches: A Morphology-Based Evaluation
Authors: Hemagirri Manisekaran , Sasidharan Sreenivasan 
Published: March 2022

AbstractPolyalthia longifolia is known for its anti-oxidative properties, which might contribute to the antiaging action. Hence, the current research was conducted to evaluate the antiaging activity of P. longifolia leaf methanolic extract (PLME) in a yeast model based on morphology using microscopic approaches. Saccharomyces cerevisiae BY611 strain yeast cells were treated with 1.00 mg/mL of PLME. The antiaging activity was assessed by determining the replicative lifespan, total lifespan, vacuole morphology by light microscopy, extra-morphology by scanning (SEM), and intra-morphology by transmission (TEM) electron microscopy. The findings demonstrated that PLME treatment significantly accelerated the replicative and total lifespan of the yeast cells. PLME treatment also delays the formation of large apoptotic-like type 3 yeast cell vacuoles. The untreated yeast cells demonstrated aging morphology via SEM analysis, such as shrinking, regional invaginations, and wrinkled cell surface. The TEM analysis revealed the quintessential aging intracellular morphology such as swollen, wrinkled, or damaged vacuole formation of the circular endoplasmic reticulum, a rupture in the nuclear membrane, fragmentation of the nucleus, and complete damaged cytoplasm. Decisively, the present study revealed the vital role of PLME in the induction of antiaging activity in a yeast model using three microscopic approaches—SEM, TEM, and bright-field light microscope.
The opportunity and limitation of legal mobilisation for social struggles: a view from the Argentinian factory recuperation movement
Authors: Meakin Jack 
Published: March 2022

AbstractThis paper examines the tension between law's opportunity to deliver social transformation and the normative limitations that shape its effectiveness as a tool of social struggle. The role of law's normative limitations on legal mobilisation strategies, or the effect of entrenched social interests on permissible legal claims, has not been properly conceptualised in legal mobilisation scholarship. In response, this paper presents a conceptual framework that comprehends the opportunity and limitation of legal mobilisation as caught in the tension between the interpretive opportunity to redetermine legal meaning and the normative deficit inherent to this task. By re-engaging with the theoretical underpinnings of legal mobilisation, we will evaluate the potential for certain types of social transformation using law and revisit the rationale for strategic legal action. We will bring together our conceptual treatment of legal mobilisation with a sobering analysis of the Argentinian factory recuperation movement's mobilisation of legal demands. The movement's relative success in confronting the legal system's commitment to private property rights and winning protections for worker co-operatives presents an opportunity to learn about the effective potential of legal strategy and the extent to which it can be used to confront the normative commitments of a legal system.
Classification of Synoptic Patterns With Mesoscale Mechanisms for Downslope Windstorms in Korea Using a Self‐Organizing Map
Authors: Shin Yewon , Kim Jung‐Hoon , Chun Hye‐Yeong , Jang Wook , Son Seok‐Woo 
Published: March 2022

AbstractDownslope windstorms (DWs) are responsible for wildfires, wind gusts, and turbulence on the lee side of the Taebaek Mountains in the Yeongdong (YD) region of Korea. We classified the synoptic conditions of the windstorms in the YD region using a self‐organizing map (SOM). For windstorm events from 1979 to 2019, sea level pressure anomalies were used to train the SOM. It was found that the synoptic patterns could be classified into three representative types: (1) the south‐high and north‐low pattern in the spring, (2) the west‐high and east‐low pattern in the winter, and (3) the strong low‐pressure system passing through the northern part of Korea. At 850 hPa, prevailing southwesterly and northwesterly flows with warm and cold advections were dominant in Types 1 and 2, respectively. And, Type 3 presented a well‐developed baroclinic system of cyclone. Adiabatic warming by downslope windstorm was the strongest in Type 1, which was likely to have a huge impact on the spread of wildfires. Three mesoscale generation mechanisms were examined under different synoptic patterns. The hydraulic‐jump theory was dominant for the windstorms in Type 2 due to upstream flows with moderate Froude numbers and inversion layers. The partial reflection of mountain waves was found in all types but was more frequent in Type 1 than others. DWs with wave breakings at critical levels mostly occurred in Type 1. This objective classification of weather patterns responsible for DWs in the YD region may improve predictions and future projections of this event, particularly in light of ongoing climate changes.