bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGGUNAAN MEDIA INFORMASI OLEH PENYULUH PERTANIAN BALAI PENGKAJIAN TEKNOLOGI PERTANIAN
Authors: Kustanti Eni , Rusmana Agus , Hadisiwi Purwanti 
Published: February 2022

<p><em>Media komunikasi dan informasi di Kementerian Pertanian RI yang merupakan bagian dari Spektrum Diseminasi Multi Channel tersedia dalam bentuk media cetak, media eletronik, internet, dan media sosial, dapat digunakan oleh penyuluh pertanian sebagai sumber informasi untuk meningkatkan kompetensinya. Penelitian ini bertujuan mengetahui </em>(1) <em>karakteristik individu penyuluh pertanian di Balai Pengkajian Teknologi Pertanian (BPTP) dan </em>(2) <em>penggunaan media informasi oleh penyuluh pertanian BPTP. Metode penelitian dilakukan secara kuantitatif menggunakan survei </em>online <em>terhadap penyuluh BPTP di 33 provinsi. Hasil penelitian menunjukkan karakteristik individu penyuluh secara usia berada pada usia produktif dan sebanyak 33 % berada pada usia mendekati pensiun, pendidikan di dominasi sarjana (S1), masa kerja penyuluh dominan berada antara 5 sampai 10 tahun dan jabatan fungsional dominan berada pada penyuluh pertanian ahli pertama. Intensitas penggunaan media informasi oleh penyuluh pertanian tergolong rendah (frekuensi kurang dari tiga kali setiap minggu dan durasi kurang dari dua jam per hari). Media sosial dan internet menjadi pilihan penyuluh untuk melakukan akses informasi dengan intensitas (frekuensi dan durasi) lebih tinggi dibandingkan media yang lain. Media internet menjadi pilihan utama oleh penyuluh terkait dengan jenis informasi pertanian yang tersedia maupun kesesuaian isi media dengan kebutuhan informasi penyuluh.</em><em></em></p>
Desain Model Bahan Ajar Berbasis Elektronik dan Minat Belajar Pendidikan Agama Islam Siswa Sekolah Dasar Negeri (SDN) Kelas Bawah di Indonesia
Authors: Nasir Muhammad , Alkhury Saifun Nizar , Rijal Muhammad Khairul 
Published: February 2022

Abstract
Religious learning in the era of information technology development, especially in formal education institutions, faces serious challenges, if the teaching materials used are the majority of printed teaching materials and teacher teaching strategies are more dominant in applying a teacher-centered approach. Thus, in this millennial era, teachers are required to be more creative and innovative in developing and designing teaching materials, media, and student-centered learning strategies to increase motivation and interest in learning. This study aims to design an electronic-based Islamic Religious Education (PAI) teaching material model to increase student interest in elementary school (SDN) in Indonesia. Theories related to this design are the characteristics of the content and teaching materials of Islamic Religious Education, constructivist learning theory, technological and humanistic curricula as well as the characteristics of lower grade Public Elementary School (SDN) students. The type of research used is research and development with data collection techniques, interviews, and observations. The research findings show that the results of limited trials and extensive trials of the design of the electronic-based teaching material model of the development results indicate a significant increase in the learning interest of low-grade Public Elementary School (SDN) students in the subject of Islamic Religious Education (PAI) after the treatment. (µ ≤ 0.05).
Change In Metabolic Health Status Over Time And Risk Of Chronic Kidney Disease: A Prospective Study
Authors: Cui Haozhe , Liu Qian , Wu Yuntao , Cao Liying 
Published: February 2022

Abstract
Objective: Metabolically healthy obesity (MHO) is a dynamic condition and is known to increase the risk for chronic kidney disease (CKD). In this study, we aimed to examine the association between metabolic health status and its change over time and CKD risk.Methods: A total of 39463 participants from Kailuan Study were collected body mass index and metabolic health status at 2006/07 and 2010/11 examination. Metabolic abnormality was diagnosed by the presence of any 2 of 4 components (elevated blood pressure, elevated fasting blood glucose, elevated triglyceride and decreased high-density lipoprotein cholesterol). We classified participants into six groups according to metabolic health status and obesity. The changes in obesity and metabolic health status were considered from 2006/07 to 2010/11. Results: Of the participants classified as MHNW or MHO at baseline, 17.25% and 39.64% were classified as MUNW or MUO in 2010/11, respectively. During a mean follow-up of 9.7 years, 5351 participants developed CKD from 2010/11. Compared with participants in the stable MHNW group, the stable MHO group had a significantly higher risk of incident CKD (HR: 1.16; 95% CI: 1.02-1.33), but was lower than that in individuals with MUO. Individuals with metabolic healthy at baseline who changed to metabolic abnormality during follow-up had higher risk of CKD. Conclusions: MHO phenotype or its transition to a metabolically unhealthy phenotype were associated with increased risk of CKD. Stable metabolic health individuals had lower risk of CKD than those with metabolically unhealthy phenotype.
LINE-1 and Alu methylation signatures in autism spectrum disorder and their function in the regulation of autism-related genes
Authors: Saeliw Thanit , Permpoon Tiravut , Iadsee Nutta , Tencomnao Tewin , Sarachana Tewarit , Green Daniel , Sae-Lee Chanachai 
Published: February 2022

Abstract
BackgroundLong interspersed nucleotide element-1 (LINE-1) and Alu elements are retrotransposons whose abilities cause abnormal gene expression and genomic instability. Several studies have focused on DNA methylation profiling of gene regions, but the locus-specific methylation of LINE-1 and Alu elements has not been identified in autism spectrum disorder (ASD). MethodsDNA methylation age was predicted using Horvath’s method. We interrogated locus- and family-specific methylation profiles of LINE-1 and Alu elements (22,352 loci) in ASD blood using publicly-available Illumina Infinium 450K methylation datasets from heterogeneous ASD (n = 52), ASD with 16p11.2 del (n = 7), and ASD with Chromodomain Helicase DNA-binding 8 (CHD8) variants (n = 15). The differentially methylated positions of LINE-1 and Alu elements corresponding to genes were combined with transcriptome data from multiple ASD studies.Results We identified the epigenetic age acceleration significantly decelerated in ASD children over the age of 11 years. We further interrogated locus- and family-specific methylation profiles of LINE-1 and Alu elements (22,352 loci) in ASD blood using publicly-available Illumina Infinium 450K methylation datasets from heterogeneous ASD (n = 52), ASD with 16p11.2 del (n = 7), and ASD with Chromodomain Helicase DNA-binding 8 (CHD8) variants (n = 15). DNA methylation profiling revealed LINE-1 and Alu methylation signatures in each ASD risk loci by which global methylation were notably hypomethylated exclusively in ASD with CHD8 variants. When LINE-1 and Alu elements were clustered into subfamilies, we found methylation changes in a family-specific manner in L1P, L1H, HAL, AluJ, and AluS families in the heterogeneous ASD and ASD with CHD8 variants. Interesting, our results showed that LINE-1 and Alu methylation within target genes is inversely related to the expression level in each ASD variant. Finally, we demonstrate the potential for LINE-1 and Alu methylation signatures to predict ASD individuals from non-ASD. ConclusionsThe DNA methylation signatures of the LINE-1 and Alu elements in ASD, as well as their functional impact on ASD-related genes, have been identified. If confirmed in future larger studies, these finding may contribute to the identification of epigenomic biomarkers of ASD in those with high risk of ASD.
Effects of different LED light spectra on growth and immunity of the Japanese eel (Anguilla japonica) and giant mottled eel (A. marmorata)
Authors: Lin Yen-Ting , Hung Wei-Chun , Yeh Yin-Fu , Lu Kuang-Mao , Cherng Ding-Hwa , Han Yu-San 
Published: February 2022

Abstract
Indoor recirculating aquaculture systems make light control possible and enable the usage of specific coloured lights to promote the growth and immunity of aquaculture species. Five different LED wavelengths (white light, red light [622 nm], green light [517 nm], blue light [467 nm], and dark) were used in this study to evaluate growth and immunity in the glass eel stage of two high-valued anguillid species, Japanese eel ( Anguilla japonica ) and giant mottled eel ( A. marmorata ). There were no significant differences in growth of the Japanese eel among the groups after ­12 weeks of feeding (P > 0.05); the survival rate of each group was over 95%. The giant mottled eel showed better growth in total length and body weight in the red light and dark groups (P < 0.05). Expression levels of immune-related genes were not significantly different between each group of the Japanese eel and the giant mottled eel (P > 0.05). The growth of the Japanese glass eel was not significantly sensitive to different LED wavelengths, while the giant mottled glass eel showed better growth under red light and dark environments. Neither eel species showed significant differences in innate immunity under different LED wavelengths.
A Disease-causing Human Osteoprotegerin Mutant Exists in Hyper-oligomerized Forms
Authors: Luo Yin , Li Miaomiao , Xu Ding 
Published: February 2022

Abstract
Recently, a human mutation of OPG was identified to be associated with familial forms of osteoarthritis. This missense mutation (c.1205A=>T; p.Stop402Leu) occurs on the stop codon of OPG, which results in a 19-residue appendage to the C-terminus (OPG+19). The biochemical consequence of this unusual sequence alteration remains unknown. Here we expressed OPG+19 in 293 cells and the mutant OPG was purified to homogeneity by heparin affinity chromatography and size exclusion chromatography. We found that in sharp contrast to wildtype OPG, which mainly exists in dimeric form, OPG+19 had a strong tendency to form higher-order oligomers. To our surprise, the hyper-oligomerization of OPG+19 had no impact on how it binds cell surface heparan sulfate, how it inhibits RANKL-induced osteoclastogenesis and TRAIL-induced chondrocytes apoptosis. Our data suggest that in biological contexts where OPG is known to play a role, OPG+19 functions equivalently as wildtype OPG. The disease-causing mechanism of OPG+19 likely involves an unknown function of OPG in cartilage homeostasis and mineralization. By demonstrating the biochemical nature of this disease-causing OPG mutant, our study will likely help elucidating the biological roles of OPG in cartilage biology. Running headline: Human osteoprotegerin mutant is highly oligomerized
Past, present, and future trends of overweight and obesity in Belgium using Bayesian age-period-cohort models
Authors: De Pauw Robby , Claessens Manu , Gorasso Vanessa , Drieskens Sabine , Faes Christel , Devleesschauwer Brecht 
Published: February 2022

Abstract
BackgroundConsidering the current overweight and obesity epidemic and its associated increase in non-communicable diseases and healthcare costs, the current study aimed to project the trends in prevalence of overweight and obesity in Belgium using a Bayesian age-period-cohort (APC) model to support policy planning.MethodsHeight and weight of 58,369 adults aged 18+ years, collected in six consecutive cross-sectional health interview surveys between 1997 and 2018, were evaluated. Criteria used for overweight and obesity were defined as body mass index (BMI) ≥ 25, and BMI ≥ 30. A Bayesian APC model was applied to evaluate past trends and associated socio-demographic risk factors, and to forecast trends to 2019-2029. All analyses were performed based on integrated nested Laplace approximation (INLA) and took the complex survey design into account.ResultsThe prevalence of overweight and obesity has increased between 1997 and 2018. If current trends continue, it is likely to that a further increase in the prevalence of overweight and obesity in the population will be seen by 2029 with a probability of growth of 51.2% and 73.3%, respectively. Forecasts indicated a potential prevalence of 50.1% [16.2%; 84.4%] in 2029 for overweight, and 21.4% [9.0%; 43.4%] for obesity. Among survey participants, middle-aged men with no higher education and a middle income showed the highest risk of overweight and obesity.ConclusionsWe projected an alarming increase in the prevalence of overweight and obesity. A decrease in cases seems very unlikely. There is an urgent need to target younger age groups for prevention and implementation of public educational programs to limit the increasing trend in overweight and obesity.
A Slope Test Strategy for Censored Data in Survival Analysis
Authors: Zhang Yumin , Chen Zheng , Hou Yawen 
Published: February 2022

Abstract
Background: Censored data are nearly ubiquitous in survival analysis. If the proportional hazard assumption is invalid, traditional test methods, such as family of log-rank test, lose power rapidly. This paper gives a new slope strategy to improve power of test. The aim of this study is to establish effective tests for censoring data which are familiar in biomedical research and medical practice. Methods: Four tests based on slope are proposed in this paper, and monte carlo simulations with various survival curves, sample size and censoring rates scenarios were performed to evaluate test power. Four common tests, log-rank test, Gehan-Wilcoxon test and Fleming-Harrington with different parameters, were compared with four new slope tests. Results: The slope tests are more effective when the censoring rate is equal to or greater than 30%, especially for scenarios involving early hazard function differences. Two clinical cases also indicate that the proposed slope methods can detect differences for real data. Conclusions: Slope tests may provide a supplement of computing method to survival curve test, it will be more useful considering higher censoring rates.
Human Serum Albumin (HSA) Regulates the Expression of Histone-like Nucleoid Structure Protein (H-NS) in Acinetobacter baumannii
Authors: Escalante Jenny , Nishimura Brent , Tuttobene Marisel R. , Subils Tomás , Pimentel Camila , Georgeos Nardin , Sieira Rodrigo , Bonomo Robert A. , Tolmasky Marcelo E. , Ramírez María Soledad 
Published: February 2022

Abstract
Acinetobacter baumannii, a common cause of nosocomial infection, easily adapts to multiple environments. These characteristics together with this bacterium’s ability to acquire antibiotic resistance determinants make it a notorious pathogen. The presence of human serum albumin (HSA) is associated to modification in levels of expression of numerous genes. The presence of HSA in the culture medium is also correlated with a reduction in levels of the global suppressor histone-like nucleoid structure protein, H-NS. Transcriptome analysis using a wild type and isogenic Δhns strains cultured in lysogeny broth, LB, in the presence or absence of HSA revealed that a subset of eleven, out of 183 genes whose expression is modified by HSA, were regulated through H-NS. Six and five of these genes were up- or down-regulated, respectively. Three of these genes have functions in quorum sensing (acdA, kar and fadD), one in quorum quenching (aidA), two in stress response (katE, ywrO), three in metabolism (phaC, yedL1, and yedL2), one in biofilm formation (csuAB), and one in β-oxidation of fatty acids (fadA). The regulation of these genes was assessed by: i) transcriptional analysis and qPCR at the transcriptional level; and ii) by determining the phenotypic characteristics of each function. The results of these studies support the hypothesis that HSA-mediated reduction of H-NS levels may be one very important regulatory circuit utilized by A. baumannii to adapt to selected environments, such as those where HSA-containing human fluids are abundant.
Is It Sufficient to Evaluate Bone Metastasis Involvement in Breast Cancer using SPECT/CT? A New Approach of SPECT/CT-Guided Targeted Bone Marrow Biopsy
Authors: Li Xiaomin , An Caixia , Zhang Wanchun 
Published: February 2022

Abstract
Objective To investigate the feasibility, safety, and clinical application value of single photon emission computed tomography/computed tomography (SPECT/CT)-guided bone marrow biopsy (BMB) in breast cancer (BC) patients with suspected bone metastases (BM) and compare its diagnostic performance for detection of BM with SPECT/CT. Methods The records of breast cancer patients referred for bone scintigraphy (BS), SPECT/CT and SPECT/CT-guided BMB from January of 2018 to June of 2021 in our hospital were retrospectively reviewed. 49 Patients were consecutively included in this study, all 49 specimens were analyzed by pathological and immunohistochemical studies.The biopsy success rate, total examination time, biopsy operation time, complications, CT radiation dose, and pathological and immunohistochemical results were recorded. The diagnostic performance based on SPECT/CT and SPECT/CT-guided BMB were compared with pathological, immunohis to chemical examinations and the results of subsequent follow-up. Results Bone samples of the sites with high uptake were obtained in all 49 patients under BMB. No severe postoperative complications occurred. Among all 49 cases, 34 specimens were positive for metastatic breast cancer (69%, 34/49), and positive for benign tissue in 15 cases (31%, 15/49). 1 case of 15 cases was subsequently diagnosed as metastatic breast cancer according to the follow-up result. SPECT/CT-guided BMB demonstrated significantly higher negative predictive value (NPV) when compared to SPECT/CT (p=0.021<0.05). Patients with differential expression of ER, PR, and HER-2 between primary lesions and metastatic lesions accounted for 12, 17, and 5 cases, respectively, and the inconsistency rates were 35.2% (12/34), 50% (17/34), and 14.7% (5/34), respectively. Molecular subtype changes occurred in 7 patients, accounting for 47% (16/34) of metastatic patients. Conclusion It is not insufficient to evaluate BM in BC patients using 99mTC-MDP SPECT/CT imaging. SPECT/CT-guided BMB was safe, accurate, and feasible and provided significantly higher sensitivity and NPV when compared to SPECT/CT for detection of BM in BC patients. In addition, our study initially showed the integrated approach that includes SPECT /CT and SPECT/CT-guided BMB, which can obtain one-stop imaging, pathological and molecular typing diagnosis in nuclear medicine department and offer important opportunities for precision treatment to improve quality of life of BC patients with BM.
Monitoring The After-Effects Of Ischemic Stroke Through EEG Microstates And Machine Learning
Authors: Wang Fang , Tian Yu-Chu , Zhang Xueying , Hu Fengyun 
Published: February 2022

Abstract
Background and Purpose: Stroke may cause extensive after-effects such as motor function impairments and disorder of consciousness (DoC). Detecting these after-effects of stroke and monitoring their changes are challenging jobs currently undertaken via traditional clinical examinations. These behavioural examinations often take a great deal of manpower and time, thus consuming significant resources. Computer-aided examinations of the electroencephalogram (EEG) microstates derived from bedside EEG monitoring may provide an alternative way to assist medical practitioners in a quick assessment of the after-effects of stroke. Methods: In this study, we designed a framework to extract microstates maps and calculate their statistical parameters to input to classifiers to identify DoC in ischemic stroke patients automatically. As the dataset is imbalanced with the minority of patients being DoC, an ensemble of support vector machines (EOSVM) is designed to solve the problem that classifiers always tend to be the majority classes in the classification on an imbalanced dataset.Results: The experimental results show EOSVM get better performance (with accuracy and F1-Score both higher than 89%), improving sensitivity the most, from lower than 60% (SVM and AdaBoost) to higher than 80%. This highlighted the usefulness of the EOSVM-aided DoC detection based on microstates parameters. ﻿Conclusion: Therefore, the classifier EOSVM classification based on features of EEG microstates is helpful to medical practitioners in DoC detection with saved resources that would otherwise be consumed in traditional clinic checks.
Influence of Moisture Content, Temperature, and Time on Free Fatty Acid in Stored Crude Palm Oil
Authors: Emebu Samuel , Osaikhuiwuomwan Omokaro , Mankonen Aleksi , Udoye Chinweike , Okieimen Charity , Janáčová Dagmar 
Published: February 2022

Abstract
Consequent to the importance of crude palm oil (CPO) to global food processing industries, and the need for quality assurance of CPO. A kinetic model that describes changes of free fatty acid (FFA) in industrially stored CPO has been developed. CPO FFA is a well-known indicator of the deterioration of CPO. The effect of initial moisture content, storage temperature, and time on CPO FFA has been investigated in this work. Specifically, statistical multi-regression models for changes in FFA and moisture content (MC) were developed at P-value < 0.05 or 95% confidence interval fence. It was found that CPO FFA increases with an increase in moisture content, temperature, and time in their linear term and in respect to decreases in their quadratic term, and interaction between moisture content and temperature. The CPO MC was also found to decrease with an increase in temperature and time and increases in the quadratic term of temperature. Although while the model for CPO FFA, based on Fisher's F-test: \({\text{F}}_{\text{m}\text{o}\text{d}\text{e}\text{l}}\left(6.80\right)<{\text{F}}_{95\text{\%}}\left(19.30\right)\), showed no lack-of-fit; that of CPO MC showed lack-of-fit, \({\text{F}}_{\text{m}\text{o}\text{d}\text{e}\text{l}}\left(13.67\right)\nless {\text{F}}_{95\text{\%}}\left(4.39\right)\). Furthermore, based on inference from the statistical model, their kinetic models were also developed. While the CPO FFA kinetic, found to be a half-order kinetic model and its other auxiliary models showed a very good fit (R2 {0.9933–0.8614} & RMSE {0.0020–3.6716}); that of CPO MC was a poorly fitted first-order kinetic model (R2 {0.9885– 0.3935} & RMSE {0.0605– 17.8501}).
Viable Closed-Loop Supply Chain Network with Considering Robustness and Risk as a Circular Economy
Authors: Lotfi Reza , Nazarpour Hossein , Gharehbaghi Alireza , Sarkhosh Seyyed Mahdi Hosseini , Khanbaba Amirhossein 
Published: February 2022

Abstract
The Viable Closed-loop Supply Chain Network (VCLSCND) is a new concept that integrates sustainability, resiliency, and agility in a circular economy. We suggest a new form of robust stochastic optimization for this problem by minimizing the weighted expected, maximum and Entropic Value at Risk (EVaR). We proposed this form to increase robustness against demand disruption and energy productivity. Finally, we located CLSC components and assigned flow in the automotive industry. The results show that the cost of VCLSCND is less than without viable and has -0.44% gap. By increasing the conservative coefficient and confidence level, decreasing the allowed maximum energy and increasing the scale of the main model, the cost function, time solution and energy consumption grow. We suggested applying the Fix-and-Optimize algorithm for producing upper bound of large-scale. As can be seen, the gap between this algorithm and the main problem for cost, energy and time solution is approximately 6.10%, -8.28%, and 75.01%.