bluuebunny/crossref_metadata_2025_split huggingface dataset:
Matrix-free human 2D organoids recapitulate duodenal barrier and transport properties
Authors: Masete Kopano Valerie , Günzel Dorothee , Schulzke Jörg-Dieter , Epple Hans-Jörg , Hering Nina A. 
Published: January 2025

Abstract
Background
Traditionally, transformed cell line monolayers have been the standard model for studying epithelial barrier and transport function. Recently, intestinal organoids were proposed as superior in recapitulating the intestine. Typically, 3D organoids are digested and seeded as monolayers on gelatinous matrix pre-coated surfaces for anchorage. As this coat could potentially act as a diffusion barrier, we aimed to generate robust human duodenum-derived organoid monolayers that do not need a gelatinous matrix for anchorage to improve upon existing models to study epithelial transport and barrier function.

Results
We characterized these monolayers phenotypically regarding polarization, tight junction formation and cellular composition, and functionally regarding uptake of nutrients, ion transport and cytokine-induced barrier dysfunction. The organoid monolayers recapitulated the duodenum phenotypically as well as functionally regarding glucose and short-chain fatty acid uptake. Tumour necrosis factor-alpha induced paracellular transport of 4-kDa Dextran and transcytosis of 44-kDa horseradish peroxidase. Notably, forskolin-stimulated chloride secretion was consistently lower when organoid monolayers were seeded on a layer of basement membrane extract (BME).

Conclusions
BME-free organoid monolayers represent an improved model for studying transcytotic, paracellular but especially transcellular transport. As BME is extracted from mice, our model furthers efforts to make organoid culture more animal-free.
Predicting hepatocellular carcinoma outcomes and immune therapy response with ATP-dependent chromatin remodeling-related genes, highlighting MORF4L1 as a promising target
Authors: Xu Chao , Liang Litao , Liu Guoqing , Feng Yanzhi , Xu Bin , Zhu Deming , Jia Wenbo , Wang Jinyi , Zhao Wenhu , Ling Xiangyu , Zhou Yongping , Ding Wenzhou , Kong Lianbao 
Published: January 2025

Abstract
Background
Hepatocellular carcinoma (HCC) continues to be a major cause of cancer-related death worldwide, primarily due to delays in diagnosis and resistance to existing treatments. Recent research has identified ATP-dependent chromatin remodeling-related genes (ACRRGs) as promising targets for therapeutic intervention across various types of cancer. This development offers potential new avenues for addressing the challenges in HCC management.

Methods
This study integrated bioinformatics analyses and experimental approaches to explore the role of ACRRGs in HCC. We utilized data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO), applying machine learning algorithms to develop a prognostic model based on ACRRGs’ expression. Experimental validation was conducted using quantitative real-time Polymerase Chain Reaction (qRT-PCR), Western blotting, and functional assays in HCC cell lines and xenograft models.

Results
Our bioinformatics analysis identified four key ACRRGs—MORF4L1, HDAC1, VPS72, and RUVBL2—that serve as prognostic markers for HCC. The developed risk prediction model effectively distinguished between high-risk and low-risk patients, showing significant differences in survival outcomes and predicting responses to immunotherapy in HCC patients. Experimentally, MORF4L1 was demonstrated to enhance cancer stemness by activating the Hedgehog signaling pathway, as supported by both in vitro and in vivo assays.

Conclusion
ACRRGs, particularly MORF4L1, play crucial roles in modulating HCC progression, offering new insights into the molecular mechanisms driving HCC and potential therapeutic targets. Our findings advocate for the inclusion of chromatin remodeling dynamics in the strategic development of precision therapies for HCC.
Impact of circulating lymphoma cells at diagnosis on outcomes in patients with newly diagnosed de novo diffuse large B-cell lymphoma
Authors: Chowdhury Sayan Mullick , Bhatta Subodh , Voorhees Timothy J. , Annunzio Kaitlin , Bond David A. , Sawalha Yazeed , Sigmund Audrey , Hanel Walter , Sehgal Lalit , Alinari Lapo , Baiocchi Robert , Maddocks Kami , Christian Beth , Jones Dan , Epperla Narendranath 
Published: January 2025

AbstractDiffuse large B-cell lymphoma (DLBCL), the most common B-cell non-Hodgkin lymphoma rarely presents with circulating lymphoma cells (CL) at diagnosis. Previous studies were limited by small sample size precluding robust analysis. Hence, we evaluated the prognostic relevance of CL cells in newly diagnosed DLBCL patients. Based on peripheral blood (PB) immunophenotyping, patients were grouped into CL + and CL−. CL was defined as detectable clonally restricted B-cells that matched the actual or expected B-cell immunophenotype of DLBCL. The primary endpoint was progression-free survival (PFS), and secondary endpoints included overall survival (OS) and diagnosis-to-treatment interval (DTI). Among the 1266 patients with DLBCL, 621 had PB flow at diagnosis, and after excluding patients not meeting eligibility criteria, 588 cases remained. Among these, 85 (15%) were CL + and 503 were CL- (85%). Patients in CL + group were younger (67 vs. 70 years, p = 0.03) with a higher proportion of non-bulky disease (85% vs. 56%, p < 0.0001), normal albumin (79% vs. 54%, p < 0.0001), and MYC/BCL2 and/or BCL6 rearrangements (18% vs. 7%, p = 0.003) compared to the CL − group. Patients with CL at diagnosis had significantly inferior PFS and OS compared with those without CL. After adjusting for factors associated with inferior PFS and OS in univariable analysis, presence of CL remained significantly associated with inferior PFS (HR = 2.04, 95%CI = 1.47–2.84, p < 0.0001) and OS (HR = 1.61, 95%CI = 1.1–2.36, p = 0.01), respectively. There was no significant difference in DTI between the two groups. Given the prognostic relevance associated with presence of CL, clinicians should consider checking PB flow at diagnosis in all newly diagnosed DLBCL patients.
Clinical predictors for surfactant retreatment in preterm infants with respiratory distress syndrome: the results of a pooled analysis
Authors: Dani Carlo , Poggi Chiara , Agosti Massimo , Bellettato Massimo , Betta Pasqua , Biban Paolo , Corvaglia Luigi , Falsaperla Raffaele , Forcellini Carlo , Gazzolo Diego , Gitto Eloisa , Gizzi Camilla , Lago Paola , Lista Gianluca , Maffei Gianfranco , Mosca Fabio , Napolitano Marcello , Scarpelli Gianfranco , Sandri Fabrizio , Trevisanuto Daniele , Vento Giovanni , Corsini Iuri , Pratesi Simone , Boni Luca 
Published: January 2025

Abstract
Background
The issue of retreatment with surfactant of infants with respiratory distress syndrome (RDS) has been poorly investigated. Our aim was to identify possible clinical predictors of the need for multiple doses of surfactant in a large cohort of very preterm infants.

Methods
Data were analyzed from three previous studies on infants born between 25+ 0 and 31+ 6 weeks of gestation with RDS who were treated with surfactant.

Results
We studied 448 infants. Among them 306 (68%) were treated with a single dose of surfactant and 142 (32%) were treated with multiple doses. Multivariable mixed effects logistic regression analysis showed that the odd of requiring multiple doses of surfactant was significantly lower in patients with higher gestational age (27–28 vs. 25–26 wks: OR 0.46, 95% C.l. 0.26–0.79; ≥29 vs. 25–26 wks: OR 0.34, 95% C.l. 0.13–0.85; overall P = 0.013), while it increased in infants born to mothers with hypertensive disorders of pregnancy (OR 2.53, 95% C.l. 1.49–4.31; P < 0.001) and with hemodynamically significant PDA (OR 2.74, 95% C.l. 1.66–4.53, P < 0.001).

Conclusions
Gestational age, hypertension in pregnancy, and hemodynamically significant PDA can predict the need for multiple doses of surfactant. Further investigation is needed to evaluate if these sub-groups of preterm infants represent specific phenotypes of RDS who deserve a peculiar surfactant treatment.
Nickel-induced labial angioedema in a pediatric patient with orthodontic braces: a case report
Authors: Leone Fabrizio , Gori Alessandra , Cinicola Bianca Laura , Coletti Giorgio , Pignataro Elia , Martina Capponi , Giulia Brindisi , Anania Caterina , Zicari Anna Maria 
Published: January 2025

Abstract
Background
Angioedema is a condition marked by sudden, intense swelling of the subcutaneous and submucosal tissues, typically associated with hypersensitivity reactions, genetic mutations, or reactions to medications. It can also result from contact with allergens such as nickel, leading to dermatitis.

Case presentation
: A 12-year-old girl presented at our Pediatric Immunology and Allergology service with recurrent labial angioedema for over a year, linked to the consumption of legumes and tomatoes, and following the use of a metal flute. Despite a nickel-positive patch test and subsequent avoidance of nickel, her symptoms persisted. Further investigations to rule out other causes of angioedema were unproductive. It was later discovered that she had been wearing a nickel-containing orthodontic device applied a year earlier. The removal of this orthodontic device led to a cessation of the angioedema episodes, highlighting nickel as the likely trigger.

Conclusions
This case underscores the importance of considering prolonged nickel exposure from dental devices as a potential cause of angioedema. For patients predisposed to nickel hypersensitivity, using nickel-free alternatives such as ceramic for orthodontic appliances is crucial. Additionally, comprehensive allergen screening, including latex testing, should be conducted before the placement of such devices to prevent similar adverse reactions.
Multi-level lag scheme significantly improves training efficiency in deep learning: a case study in air quality alert service over sub-tropical area
Authors: Tam Benedito Chi Man , Tang Su-Kit , Cardoso Alberto 
Published: January 2025

AbstractIn environmental monitoring, deep learning models are used where we can either use past observations or extrapolated values with high uncertainty as input. The lag scheme is commonly applied during the modeling and construction process, in the application of deep learning models in multivariate time series prediction. For an adaptive feature engineering, an automated lag scheme is essential for improving the training efficiency. In multivariate time series (MTS) models, the predictive accuracy of artificial neural network ANN-type models can be improved by including more features. It is assumed that when processing a certain number of multivariate features, the timeliness and lag time of the inter-influencing between any pair of elements are different. This research aims to adopt an adaptive approach to solve it, namely, multi-level lag scheme. The research methods include literature review, searching for relevant technology frontiers, feasibility studies, selection and design solutions, modeling, data collection and pre-processing, experiments, evaluation, comprehensive analysis and conclusions. In proof of concept, we demonstrated a practical case of seasonal ANN type MTS model and public service on air quality. In terms of models, ANN type models were attempted with ARIMA as the comparing baseline. We used public data set of more than two base stations with pollution varying from low to high and including southern to northern district of a small city. Conclusions can be drawn from the analysis of multiple experimental results, proving that the proposed solution can effectively improve the training efficiency of the model. This is of great significance, so that most such models can be implemented to adaptively use lagged past measured data as input, instead of synchronously inputting future prediction values, which can greatly improve the practical application of the model in predictive ability.
Assessment of constructed wetland projects as a multifunction landscape: a case study in Egypt
Authors: ElMeligy Aya , Gaber Rasha Mahmoud , Mostafa Hind , Haron Ahmed , Ismaeel Walaa S. E. 
Published: January 2025

AbstractThis research aims to develop a conceptual framework and assessment tool to assess sustainability of Multifunction Constructed Wetlands Projects (MCWP). First, by literature review to analyze the main points and identify the gaps in existing research to what concerns viewing constructed wetlands as multifunction sustainable landscape projects. To assess the performance of MCWP, urban sustainability indicators are proposed examining interconnections between environmental, economic and social aspects and their effects on each other. 12 environmental, 9 socio-cultural and 7 economic indicators are selected according to their relevance to the United Nations and National Sustainable Development Goals, the impacts of their weights according to a distributed questionnaire showed these percentages: environmental aspects 42%, Socio-cultural aspects 29% and the economic aspects 28%. Also, performance-oriented assessment tools for MCWPs were designed for wastewater treatment. The impacts of proposed indicators are then assessed using the adapted Leopold Matrix method. Hence, this study aims to establish an assessment model to evaluate the sustainability features of MCWPs, by proposing sustainability indicators to be assessed by measurement metrics and respective weights for indicators and sub-indicators.
Sexual dimorphism in basal and post stress parameters of neuro-endocrine-immune complex, metabolome, electrocardiogram, and gastric mucosa at rats
Authors: Fihura Oksana , Korda Mykhaylo , Klishch Ivan , Ruzhylo Sofiya , Melnyk Oksana , Zukow Walery , Yanchij Roman , Vorobienko Alyona , Plyska Oleksandr , Popovych Dariya , Popovych Igor 
Published: January 2025

Introduction and aim. Previously, we found significant associations between sex index and a number of parameters of the neuro-endocrine-immune complex and metabolome. Therefore, the next goal was a detailed analysis of sexual dimorphism in these parameters in baseline and post stress situations.
Material and methods. The experiment conducted on the same 18 males and 20 females rats Wistar line. 10 animals remained intact and other rats after a week of tap water (n=10) or phytoadaptogen “Balm Truskavets’” (n=18) administration were exposed to water-immersion and restraint stress (WIRS). The next day after stressing, endocrine, immune and metabolic parameters as well as ECG and damage to gastric mucosa was recorded.
Results. By the method of discriminant analysis was selected 23 variables (4 endocrine, 6 immune, 9 metabolic as well as 4 markers of damage to gastric mucosa and myocardium) whose constellation is characteristic for each group. The distance between the centroids of the major discriminant root of intact females and males as a measure of sexual dimorphism is 16.2 units. Acute stress increases it in control rats to 23.4 units, and in pretreated with phytoadaptogen - up to 29.4 units. Acute stress increases the severity of sexual dimorphism also in relation to variables, information about which is condensed in the minor root - from 0.99 to 2.29 units, while preventive use of phytoadaptogen limits it to 1.63 units.
Conclusion. In intact rats, significant sex differences were found for a number of endocrine, immune, and metabolic variables, which increase under the influence of acute stress per se, and to an even greater extent against the background of preventive use of a phytoadaptogen.
Where does the panic attack come from... – characteristics and forms of therapy for anxiety disorders
Authors: Grzech Patrycja , Zubrzycka Weronika , Gmyz Joanna 
Published: January 2025

Introduction and purpose: Anxiety is a term with primarily psychological meaning, describing a broadly defined, indefinite emotional state characterized by the experience of uncertainty, apprehension, fear and annoyance. Anxiety is a feature of many common diseases as well as mental disorders. It is a non-specific and common symptom in medicine occurring as a trait in personality structure, psychotic anxiety or a condition in psychosomatics. Due to the variety of clinical symptoms accompanying anxiety disorders, they should not be considered as a whole, as this group is not homogeneous and each individual differs in significant ways from the others. The following work aims to present the types of anxiety that occur, analyze their characteristics, and apply knowledge from psychology, neuroscience and psychiatry in understanding and treating these disorders. Description of the state knowledge: A review of the reviewed literature indicates the significant benefits of combining appropriately selected pharmacotherapy and concurrent psychotherapy in patients struggling with anxiety disorders. Summary: Anxiety disorders are the most common group of mental disorders that usually begin just before or in early adulthood. Although anxiety disorders are defined as the presence of extreme fear and anxiety, they encompass a range of different disease entities. The new ICD-11 International Classification of Diseases in the field of psychiatry has proposed moving away from defining a group of these disorders as “neurotic disorders” and replacing it with the less stigmatizing term “anxiety disorders” containing seven specific subgroups of disorders. The key role is to make a correct diagnosis of the disorders in question and to include effective treatment due to the wide range of anxiety as apsychopathological symptom.
The Role of Coenzyme Q10 in Modern Medicine: Insights into Energy Metabolism and Antioxidant Therapy
Authors: Górski Mateusz , Załęska Adrianna , Bychowski Mateusz , Kwaśna Julia , Kaźmierczyk Izabela , Lenart Kacper , Homza Michał , Zakrzewska Natalia , Bednarek Szymon , Kulicka Joanna 
Published: January 2025

Background: Coenzyme Q10 (CoQ10), a key component of mitochondrial energy production and a potent antioxidant, plays an essential role in addressing oxidative stress and mitochondrial dysfunction. Its applications span cardiovascular health, infectious diseases, neurological conditions, reproductive health, and fatigue management. However, gaps remain in understanding its optimal dosing, long-term impact, and efficacy across diverse health conditions.

Methods: This review synthesized findings from clinical trials, meta-analyses, and observational studies. A systematic search of PubMed and ScienceDirect was conducted, selecting studies based on methodological rigor and relevance to CoQ10’s mechanisms, efficacy, and safety.

Results: CoQ10 supplementation reduced cardiovascular mortality, alleviated migraine symptoms, improved mitochondrial function, and decreased oxidative stress. Promising but inconclusive evidence supports its role in managing statin-induced myopathy and enhancing reproductive outcomes. CoQ10 was consistently shown to be safe and well-tolerated.

Conclusion: CoQ10 demonstrates significant therapeutic potential across several health conditions. Its antioxidant and bioenergetic benefits are well-documented, but further research is needed to refine dosing strategies and evaluate long-term effects. CoQ10 remains a promising complementary therapy for modern health challenges.
Career Adaptability and Career Construction as Mediating Variables Between Hardiness and Vocational Identity
Authors: Merino-Tejedor Enrique , Serrano-Fernández María José , Boada-Cuerva Maria , Sora Beatriz 
Published: January 2025

Background: The Career Construction Theory (CCT) focuses on the active role people can play when they create and design their singular paths for career success. Unlike other career guidance theories that focus their attention on identifying vocational interests or on the fit between the person and the work settings, CCT raises the possibility that people can go beyond the determinants of their life. This study tested the adaptation model proposed by Career Construction Theory. Consolidation of vocational identity is particularly important at the university stage, in which people decide their first steps about their professional future.
Method: Participants were 1023 students from Spain and Brazil. The Spanish subsample was composed of 602 participants, 34% were men (N = 207), and 66% were women (N = 395). The average age was 21.69. The Brazilian subsample was composed by 421 participants, 39% were men (N = 165), and 61% were women (N = 256), with an average age of 24.84. The four dimensions in the model were each operationally defined by a single indicator. The Hardiness Scale represented adaptive readiness. The Career Adapt-Abilities Scale represented adaptability resources. The Student Career Construction Inventory represented adapting responses. And finally, The Vocational Identity Status Assessment represented the adaptation result.
Results: Bivariate correlations obtained between the measures were as expected by the theoretical model. All variables were significantly related to each other, and the values of the correlations were positive and quite high in both the Spanish and Brazilian subsamples. Structural Equation Modeling analysis of data indicated that the relationship between hardiness and vocational identity was mediated by both career adaptability and career construction. The overall fit indices for confirmatory factor analysis (CFAs) and structural equation models (SEMs) showed that the multiple factor structure models did not fit the data as well as the second-order structure model for hardiness, career adaptability and career construction. The multiple factor solutions only provided a better adjustment compared to the second-order solution for vocational identity.
Conclusions: The analysis supported empirically the four-dimension model proposed by the Career Construction Theory. This major finding suggests new pathways to improve individual decision-making about work and career.
PEDAGOGICAL-TEACHING METHODS AND STRATEGIES IN MANAGEMENT AND TEACHING
Authors: Morgado Elsa Maria Gabriel , Victor F. A. Barros Victor F. A. , Rodrigues João B. , Maria Beatriz Licursi Maria Beatriz , Fernandes Levi Leonido 
Published: January 2025

A presente edição temática da revista “Brazilian Journal of Education, Technology and Society (BRAJETS)” pretende apresentar uma abordagem interdisciplinar direcionada a várias abordagens nos domínios que compreendem a gestão, as didáticas, a literatura, a cultura, a pedagogia e o ambiente, tendo como ponto de sustentação a Educação sob o ponto de vista plural e holístico do termo. Apercebemo-nos nesta partilha de experiências, de uma preocupação latente em torno de reflexões a respeito da educação nas suas diversas áreas e cruzamentos disciplinares, a par das políticas públicas de inclusão. Aferir, mesclar e entrecruzar modelos, conceitos e abordagens metodológicas e investigativas de forma aplicada e com diretas implicações na atividade pessoal e profissional dos investigadores e docentes desta área especificas. Torna-se emergente experimentar e aprender, diante de múltiplos entendimentos sociais em proveito do progresso de um sistema educacional abrangente e atento às diferenças, aos desafios e essencialmente com os olhos postos no futuro da humanidade que, invariavelmente passa, como sempre assim foi e será, por uma Educação de referenciais de excelência.