bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immune‐responsive biodegradable scaffolds for enhancing neutrophil regeneration
Authors: Kerr Matthew D. , McBride David A. , Johnson Wade T. , Chumber Arun K. , Najibi Alexander J. , Seo Bo Ri , Stafford Alexander G. , Scadden David T. , Mooney David J. , Shah Nisarg J. 
Published: March 2022

AbstractNeutrophils are essential effector cells for mediating rapid host defense and their insufficiency arising from therapy‐induced side‐effects, termed neutropenia, can lead to immunodeficiency‐associated complications. In autologous hematopoietic stem cell transplantation (HSCT), neutropenia is a complication that limits therapeutic efficacy. Here, we report the development and in vivo evaluation of an injectable, biodegradable hyaluronic acid (HA)‐based scaffold, termed HA cryogel, with myeloid responsive degradation behavior. In mouse models of immune deficiency, we show that the infiltration of functional myeloid‐lineage cells, specifically neutrophils, is essential to mediate HA cryogel degradation. Post‐HSCT neutropenia in recipient mice delayed degradation of HA cryogels by up to 3 weeks. We harnessed the neutrophil‐responsive degradation to sustain the release of granulocyte colony stimulating factor (G‐CSF) from HA cryogels. Sustained release of G‐CSF from HA cryogels enhanced post‐HSCT neutrophil recovery, comparable to pegylated G‐CSF, which, in turn, accelerated cryogel degradation. HA cryogels are a potential approach for enhancing neutrophils and concurrently assessing immune recovery in neutropenic hosts.
Simultaneous detection of sepsis host response biomarkers in whole blood using electrochemical biosensor
Authors: Tanak Ambalika S. , Sardesai Abha , Muthukumar Sriram , Prasad Shalini 
Published: March 2022

AbstractSepsis is a silent killer, caused by a syndromic reaction of the body's immune system to an infection that is typically the ultimate pathway to mortality due to numerous infectious diseases, including COVID‐19 across the world. In the United States alone, sepsis claims 220,000 lives, with a dangerously high fatality rate between 25% and 50%. Early detection and treatment can avert 80% of sepsis mortality which is currently unavailable in most healthcare institutions. The novelty in this work is the ability to simultaneously detect eight (IL‐6, IL‐8, IL‐10, IP‐10, TRAIL, d‐dimer, CRP, and G‐CSF) heterogeneous immune response biomarkers directly in whole blood without the need for dilution or sample processing. The DETecT sepsis (Direct Electrochemical Technique Targeting Sepsis) 2.0 sensor device leverages electrochemical impedance spectroscopy as a technique to detect subtle binding interactions at the metal/semi‐conductor sensor interface and reports results within 5 min using only two drops (~100 μl) of blood. The device positively (r >0.87) correlated with lab reference standard LUMINEX for clinical translation using 40 patient samples. The developed device showed diagnostic accuracy greater than 80% (AUC >0.8) establishing excellent specific and sensitive response. Portable handheld user‐friendly feature coupled with precise quantification of immune biomarkers makes the device amenable in a versatile setting providing insights on patient's immune response. This work highlights an innovative solution of enhancing sepsis care and management in the absence of a decision support device in the continuum of sepsis care.
Student counsellors’ experiences of mindfulness as a component of their person‐centred counselling training: An interpretative phenomenological analysis
Authors: Holt Elizabeth 
Published: March 2022

AbstractAimMindfulness is increasingly integrated into counselling and psychotherapy practices, as well as being introduced to students in academic institutes with the aim of supporting them to balance the responsibilities of academic study, placements and other commitments alongside university life. Despite mindfulness routinely finding its way into counselling settings and being incorporated into counsellor training, there has yet to be any research conducted to explore the experience of student counsellors who have received mindfulness as a part of their undergraduate person‐centred training. This study explored the reflections of counsellors who had attended a mindfulness module during the first year of a counselling degree.DesignSix students completing a university‐based undergraduate degree in Counselling and Psychotherapy in the North‐West of England, in the UK, participated in the research. The study consisted of two students from each of the three years of the programme. Participants individually attended a semi‐structured interview to explore their experiences of mindfulness as a mandatory module of their training in person‐centred counselling. Interpretative phenomenological analysis (IPA) was used to analyse the generated data.FindingsFour superordinate themes were generated: 1) becoming a counsellor, 2) person‐centred mindfulness, 3) time, and 4) learning and development.DiscussionExperiential themes were explored including the process of professionalism, vulnerability, embodiment of person‐centred theory and the core conditions and the conflict of approach.ConclusionRecommendations are made for mindfulness to be included in counselling training programmes with a person‐centred focus. Suggestions for further research include longitudinal studies to follow the development of trainees over time.
Growth hormone isoform testing in capillary dried blood spots: Results from single and multiple dose administration studies and large‐scale field collections
Authors: Miller Geoffrey D. , Husk Jacob , Crouch Andre K. , Eichner Daniel 
Published: March 2022

AbstractA multiphase study was designed to examine the detectability of human growth hormone (GH) use in capillary dried blood spots (DBS). First, 13 subjects self‐injected a single, 2‐mg dose of somatropin and collected capillary DBS samples for 24 h. Next, nine subjects self‐injected 2‐mg somatropin, six times over the course of 11 days; DBS were collected intermittently following dosing. Finally, a nondrug, large‐scale field study involved DBS collections from an athlete and staff population over 3 years. All DBS samples were self‐collected using the Tasso M20 device and were analyzed for the presence of GH using the WADA‐approved GH isoforms test. Following the single dose, positive detection within 12 h of dosing was 86% and 56% sensitive on Kits 1 and 2, respectively. In the multidose study, detection within 12 h was 85% and 69% sensitive on Kits 1 and 2, respectively. No positives were detected outside the 12‐h window following a single dose, wherein detection was 5.6% sensitive at 24‐h in the multidose study. Combining the 12‐h windows from both studies, 100% of samples had measurable recombinant (REC) and pituitary (PIT) GH concentrations above the assay LoD, 0.041 ng/ml. Finally, 1213 samples were collected in the large‐scale field study: 189 showed REC and PIT concentrations above the LoD; none returned positive results. GH is detectable in capillary DBS using the isoforms method for 12–24 h following use. While detection is short lived, transitioning to a DBS self‐collection method can allow more frequent testing and increase deterrence to GH abuse.
High‐frequency and long‐term observations of eDNA from imperiled salmonids in a coastal stream: Temporal dynamics, relationships with environmental factors, and comparisons with conventional observations
Authors: Searcy Ryan T. , Boehm Alexandria B. , Weinstock Chloe , Preston Christina M. , Jensen Scott , Roman Brent , Birch James M. , Scholin Christopher A. , Van Houtan Kyle S. , Kiernan Joseph D. , Yamahara Kevan M. 
Published: March 2022

AbstractA greater understanding of eDNA behavior in the environment is needed before it can be employed for ecosystem monitoring applications. The objectives of this study were to use autonomous sampling to conduct long‐term, high‐frequency monitoring of the eDNA of native salmonid species in a Californian coastal stream, describe temporal variation of eDNA on multiple scales and identify environmental factors that drive this variation, and evaluate the ability of the eDNA datasets to detect rare species and represent organismal abundance. Using high‐throughput autonomous environmental sample processors (ESPs) and qPCR, we enumerated eDNA concentrations from 674 water samples collected at subdaily intervals over 360 days at a single site. We detected eDNA from two imperiled salmonids (coho salmon Oncorhynchus kisutch and steelhead/rainbow trout O. mykiss) in most samples; O. kisutch eDNA was generally in lower concentration and more variable than O. mykiss eDNA. High‐frequency (i.e., subdaily and daily) variability in salmonid eDNA concentrations showed occasional patchiness (i.e., large differences between consecutive samples), while seasonal differences were observed consistent with the ecology of the species at this site. Salmonid eDNA concentrations were significantly associated with creek discharge, photoperiod, and whether the creek mouth was open or closed by a seasonal sandbar. The release of hatchery‐origin O. kisutch parr into the stream was associated with a significant increase in eDNA concentration for the remainder of the study. We compared eDNA signals with fish abundance data collected from traps located at the site. Fish were detected more often by eDNA than from trapping. Significant positive associations between fish abundance and eDNA concentrations were observed for O. mykiss; however, no such associations were observed for O. kisutch. This study adds to our knowledge on the occurrence and behavior of fish eDNA in lotic systems and informs future biomonitoring efforts using automated sampling technology.
Enhanced carbonation curing of cement pastes with dolomite additive
Authors: Guo Ruonan , Wang Lei , Huang Hao , Wang Tao , Yi Zhenwei 
Published: March 2022

AbstractCarbonation curing of cement‐based materials has recently received increasing attention as a CO2 utilization technology. This study aimed at investigating the effects of carbonation curing on the performance of ordinary Portland cement (OPC) pastes with dolomite additive (DPC). The CO2 uptake capacity, after being normalized to carbonation active components, significantly increased with larger dolomite mixing ratios. For DPC‐25% samples under 2.5 MPa curing pressure, the maximum CO2 uptake capacity reached 23.6 wt%, which was 23% higher than that of pure OPC samples under the same condition. Effects of water to solids (w/s) ratio and temperature on carbonation are two‐sided. The optimum w/s ratio for CO2 uptake capacity of DPC‐15% samples was approximately 0.20, while the optimum temperature was equal to 60℃ or higher than 60℃. The CO2 uptake capacity increased with finer particle size and higher CO2 curing pressures. Compared to large particles, smaller particles are more likely to have a better dilution effect, providing more contact surface for carbonated precipitation. From the pore structure changes perspective, carbonation products filled the interface between the dolomite and amorphous particles. DPC‐25% samples with higher dolomite mixing ratios provided more pores and pathways for gas diffusion, and exhibited a more uniform structure, thereby contributing to the highest compressive strength values of DPC‐25% samples (63.8 MPa) among all the DPC samples. These findings imply the possible feasibility of dolomite as an additive in carbonation cured building materials. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
The ability of U.S. macroeconomic variables to predict Asian financial market returns
Authors: Tzeng Kae‐Yih 
Published: March 2022

AbstractThis study conducts an extensive empirical investigation of whether 20 U.S. macroeconomic variables influence Asian financial market returns. The in‐sample results show that certain U.S. macroeconomic variables, such as the commercial paper‐to‐Treasury‐bill spread or the manufacturing Purchasing Managers' Index, possess powerful abilities to predict Asian market returns. The out‐of‐sample results show that other macroeconomic variables, such as change in the inflation rate, also show predictive power. We also use combination methods that pool information from U.S. macroeconomic variables to improve their predictive ability, and find that doing so produces superior out‐of‐sample results. We therefore find that the 20 U.S. macroeconomic variables contain information that can be used to predict Asian market returns.
Degree of polarization uniformity for dental calculus visualization
Authors: Hsiao Tien‐Yu , Ho Yi‐Ching , Lee Shyh‐Yuan , Sun Chia‐Wei 
Published: March 2022

AbstractDuring the treatment for periodontitis, the removal of dental calculus is essential. Previously, we have proposed the DAM algorithm for intuitive identification of the site of lesion, enabling the non‐contact assessment during the operation. Nonetheless, the delineation of dental calculus was still imperfect. To this end, here we utilized the power of polarization‐sensitive optical coherence tomography and evaluated the contrast called degree of polarization uniformity for dental calculus visualization. The result showed that the selected index demonstrated excellent contrast of dental calculus from other normal dental hard tissues. The proposed contrast is promising for accurate dental calculus delineation.
Bone Mineral and Organic Properties in Postmenopausal Women Treated With Denosumab for Up to 10 years
Authors: Farlay Delphine , Rizzo Sébastien , Dempster David W , Huang Shuang , Chines Arkadi , Brown Jacques P , Boivin Georges 
Published: March 2022

ABSTRACT
In postmenopausal women with osteoporosis, denosumab (DMAb) therapy through 10 years resulted in significantly higher degree of mineralization of bone, with a subsequent increase from years 2–3 to year 5 and no further difference between years 5 and 10. Our aim was to assess the variables reflecting the quality of bone mineral and organic matrix (Fourier transform infrared microspectroscopy), and the microhardness of bone (Vickers microindentation). Cross-sectional assessments were performed in blinded fashion on iliac bone biopsies from osteoporotic women (72 from FREEDOM trial, 49 from FREEDOM Extension trial), separately in cortical and cancellous compartments. After 2–3 years of DMAb, mineral/matrix ratio and microhardness of cortical bone were significantly higher compared with placebo, whereas mineral maturity, mineral crystallinity, mineral carbonation, and collagen maturity were not different in both bone compartments. Through 5 years of DMAb, mineral carbonation was significantly lower and mineral/matrix ratio, mineral maturity, and crystallinity were significantly higher versus 2–3 years and were not different between 5 and 10 years, with the exception of mineral maturity in cancellous bone. These data support a transition of mineral to more mature crystals (within physiological range) and the completeness of secondary mineralization within 5 years of DMAb treatment. Microhardness in cortical and cancellous compartments was significantly lower at 5 years of DMAb versus 2–3 years and was not different from years 5 to 10. The lower microhardness at years 5 and 10 is likely the result of maturation of the organic matrix in a persistently low state of bone remodeling over 5 and 10 years. © 2022 American Society for Bone and Mineral Research (ASBMR).
Methane production of rare earth element‐rich Dicranopteris dichotoma and effects of La(III) on anaerobic digestion performance of lignocellulose
Authors: Xing Tao , Wang Zhi , Zhen Feng , Liu Huiliang , Wang Fei , Zhang Yi , Kong Xiaoying , Sun Yongming 
Published: March 2022

AbstractBACKGROUNDPhytoremediation by hyperaccumulators (such as Dicranopteris dichotoma) is widely used for the remediation of rare earth tailings in China. Accordingly, a large amount of rare earth‐rich plant residues is produced. Anaerobic digestion could convert biomass into energy. In this study, the anaerobic digestion performance of D. dichotoma, which is rich in rare earth elements (REEs), was investigated. Cellulose, xylan, and glucose served as the model substrates to explore the effects of La(III) on hydrolysis and methane production using batch anaerobic digestion.RESULTSThe results indicated that the La(III) concentration of 500 mg/L inhibited methane production from cellulose with the reduciton of 20%. Inversely, no significant difference was found in the cumulative methane yield from glucose with different La(III) concentrations. The microbial community results showed that the lack of cellulose hydrolyzing bacteria, such as Clostridium III and Clostridium XlVa, may be the main reason for the inhibition of methane production. Furthermore, the methane yield of REE‐rich D. dichotoma was 181.5 ± 14.6 mL/g VS and the La(III) concentration of luquid digestate was only 2.20 mg/L.CONCLUSIONThe inhibition of La(III) on anaerobic digestion of lignocellulose was mainly in the hydrolysis process of cellulose, while the inhibition of xylan hydrolysis was relatively weak, and it had no significant effect on methanogenesis process of glucose. La(III) showed a small effect on the methane production of REE‐rich D. dichotoma due to a small amount of release into the liquid digestate. Anaerobic digestion may be a feasible way for treating lignocellulose plants contaminated with rare‐earth minerals. © 2022 Society of Chemical Industry (SCI).
Critical thinking disposition among Thai dental students
Authors: Sireerat Kittichai , Seki Naoko , Akiyama Masako , Kinoshita Atsuhiro , Morio Ikuko 
Published: March 2022

AbstractObjectivesCritical thinking is recognized as an essential aspect in the training of healthcare professionals, yet it has not been explicitly applied to dental education in Thailand. Therefore, this research study aimed to survey the status of critical thinking disposition among Thai dental students and examine the correlation between critical thinking disposition and academic performance.MethodsIn 2020, 322 students from a Thai dental school were invited to participate in the study and answer an online questionnaire supplying demographic data and Grade Point Average (GPA), and respond to the California Critical Thinking Disposition Inventory (CCTDI). A one‐way analysis of variance and Spearman's correlation were used to analyze the data.ResultsOverall, 279 of the 322 participants (86.6%) completed the demographic information and CCTDI. Three subscales were found to be in the ambivalent range: truth‐seeking (36.8 ± 6.2), systematicity (39.0 ± 5.5), and maturity of judgment (37.1 ± 5.9). Most of the CCTDI subscale scores did not significantly correlate with GPAs, except for systematicity (r = 0.119, p = 0.047) and confidence in reasoning (r = 0.146, p = 0.015).ConclusionThis study examined the strengths and weaknesses of the students’ critical thinking dispositions. Their GPAs correlated with two critical thinking disposition subscales; however, the results did not guarantee a correlation with academic outcomes. Pedagogical strategies to develop dental students’ critical thinking dispositions need to be further assessed.
A selective chromone‐based colorimetric chemosensor for detecting Cu2+ in near‐perfect aqueous solution and test kit
Authors: Gil Dongkyun , Kim Cheal 
Published: March 2022

AbstractA colorimetric chemosensor CMF (N′‐[(E)‐(4‐oxo‐4H‐chromen‐3‐yl)methylidene]furan‐2‐carbohydrazide) was applied for detecting Cu2+. CMF showed colorimetric selectivity to Cu2+ through a color variation from colorless to yellow. Binding ratio between CMF and Cu2+ was analyzed by Job plot to be a 2:1. Limit of detection was found to be 0.38 μM. The practicality of CMF was illustrated by the quantification of Cu2+ in real samples. In particular, the CMF‐coated test kit was able to detect the copper ions at a concentration lower than the recommended concentration (31.5 μM) of the World Health Organization (WHO) for copper ion. The binding mode of CMF and copper ions was studied through UV–visible spectroscopy, 1H NMR titration, ESI‐mass analysis, and DFT calculations. Sensing mechanism of CMF to Cu2+ was proposed to be the ligand‐to‐metal charge transfer (LMCT) through DFT calculations.
Design, synthesis, insecticidal activities, and molecular docking of novel pyridylpyrazolo carboxylate derivatives
Authors: Yang Shuai , Peng Hongxiang , Zhu Jinyi , Zhao Chen , Xu Hanhong 
Published: March 2022

AbstractBased on our previous work, using the strategies of “scaffold hopping” and “intermediate derivatization method” (IDM), a total of 44 novel pyridylpyrazolo carboxylate derivatives were designed and synthesized. The structures of these compounds were identified by 1H NMR and 13C NMR, and the insecticidal activities of the target compounds against Plutella xylostella and Spodoptera frugiperda were tested. Compound G35 showed the best insecticidal activities against P. xylostella (LC50 = 33.65 mg/L) and S. frugiperda (mortality rate = 61.21% at 100 mg/L). Preliminary structure–activity relationship analysis showed that the introduction of amino group and acetamide on the benzene ring helped to improve the insecticidal activity of the scaffold. Molecular docking model between compounds G34 or G35 and Ryanodine receptors (RyRs) of P. xylostella showed that RyRs may be a potential target of this series compounds and explained the difference in insecticidal activities. These findings provided guidance for further optimization of the pyridylpyrazolo carboxylate scaffold as potential insecticide.
Employee voice and coworker support: The roles of employee job demands and coworker voice expectation
Authors: Liu Xin , Zheng Xiaoming , Ni Dan , Harms Peter D. 
Published: March 2022

SummaryAlthough a number of studies have examined the antecedents of employee voice, knowledge on the consequences of voice, especially in terms of the coworkers' responses, is limited. The current research addresses this important issue by examining when and why employee voice, as a citizenship behavior, can facilitate coworker support. Drawing on expectancy violation theory, we propose that higher job demands strengthen the positive relationship between employee voice and coworker support by decreasing coworker voice expectation. We tested our hypotheses using two complementary studies: Study 1 adopted an experience sampling methodology and collected data consisting of 944 daily responses from 103 employees over a 10‐day period; Study 2 adopted a two‐wave survey design and collected data from 161 employee–coworker dyads. The results support our hypotheses that higher (versus lower) job demands enhance the positive association between employee voice and coworker support via decreased (versus increased) coworker voice expectation.