bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinico-Hematological Study of Thrombocytopenia on the Basis of Bone Marrow Examination in a Tertiary Care Hospital
Authors: Nagar Vaidehi , Patil Nanda , Kanetkar Sujata 
Published: February 2022

Background: Thrombocytopenia can occur in a variety of clinical conditions. Bone marrow aspiration is crucial for diagnosis. The purpose of this study was to investigate the causes of thrombocytopenia, its spectrum, and how to interpret the results of bone marrow aspiration.
Aim & Objectives: To study the cases of thrombocytopenia on the basis of bone marrow examination for correlation of bone marrow findings with clinical features, haematological parameters and other investigations in cases of thrombocytopenia. Secondary objective is to investigate the role of morphological examination of bone marrow aspiration and trephine biopsy in diagnosis of various disorders leading to thrombocytopenia.
Results: The cross-sectional prospective study lasted two years, from May 2019 to May 2021, at a tertiary care centre where clinico-haematological evaluation of patients with thrombocytopenia was done on the basis of bone marrow examination. A total of 103 cases with thrombocytopenia underwent bone marrow examination of which most patients belonged to the age group of 41-50 with a male preponderance. Majority of the patients presented with generalized weakness and pallor. Megaloblastic anaemia was the most predominant cause followed by acute myeloid leukemia and dimorphic anaemia. More research into the pathogenesis of megakaryocytic alteration and its contribution to thrombocytopenia could lead to a better understanding of the pathogenesis of a variety of hematopoietic disorders and the identification of broader clinical applications.
Patients’ Satisfaction with Healthcare Services at Private and Public Hospitals in Aseer Region
Authors: Ozam Tariq Saad , Garnan Asma Abdullah S. , Alqahtani Naif  M. 
Published: February 2022

Assessments of patients’ satisfaction with provided health care is important, not only as a measure of the quality of care patients receive, but also in identifying potential areas for improving the content of care provided by physicians .Research proved that improving patient satisfaction with physician increases the likelihood that a patient will return to a given health care provider.
Aim of Study: To assess patients’ satisfaction with healthcare services at private and governmental hospitals in Aseer Region, 2020.
Methodology: Following a cross sectional design, two hospitals were included, a governmental hospital and a private hospital. A total of 400 patients were interviewed according to a consecutive sampling technique
Conclusions: In both public and private hospitals, quality of provided health services is significantly less than that expected across all components of patients’ satisfaction. In public hospitals, higher quality of received health services is perceived by older patients. In private hospitals, higher quality of received health services is v older and male patients and those who have health insurance. Both received and expected quality of health services are significantly higher in private than public hospitals.
Evaluation of Activated Partial Thromboplastin Time and Prothrombin Time in HIV and TB Patients in Owerri Metropolis
Authors: Okoroiwu Ijeoma Leticia , Obeagu Emmanuel Ifeanyi , Anaebo Queen Braxton N. , Walter Olivia 
Published: February 2022

Background: Human immunodeficiency Virus (HIV) and Tuberculosis (TB) are the leading infectious diseases with high morbidity and mortality in the developing countries; it has been known to be associated with some coagulation abnormalities especially as the disease progresses.
Aim: The study aimed at evaluating the effect of HIV- Tuberculosis co- infection on some haemostatic parameters (APTT & PT). It was carried out at Federal Medical Centre Owerri, Imo state.
Materials and Methods: Sixty (60) subjects were sampled comprising fifteen (15) HIV positive subjects, fifteen (15) TB positive subjects, fifteen (15) HIV-TB co-infected subjects and fifteen (15) HIV and TB negative subjects (Control). Activated Partial Thromboplastin Time (APTT) and Prothrombin Time (PT) were analyzed using standard techniques.
Results: The results showed that HIV positive subjects showed a significant values of PT (15.45±1.44sec, P < 0.05) when compared with control subjects (12.45 + 1.23) and a non-statistically significant values of Activated Partial Thromboplastin Time (APTT) (33.33± 6.27sec, P > 0.05) when compared with the control subjects (29.05+2.19). TB subjects showed statistical significant values of PT (15.84±0.98scc, P < 0.05) when compared with control subjects (12.45 + 1.23) and non- statistical significant values of APTT (33.55+5.26 sec P > 0.05) when compared with the control subjects (29.05+2.19 sec). Both the PT and APTT of HIV- tuberculosis Co-infected subjects showed significant values (17.03±1.46sec; 37.59±4.69sec, P < 0.05) compared with the control subjects (12.45+ 1.23sec; 29.05+2.19). One way analysis of variance showed no significant difference of a PT of TB patient (15.84+0.98) compared to PT of HIV patients (15.45+1.44 sec). PT of HIV- TB co-infected subjects higher values (P< 0.05) when compared with HIV patients but not significantly (P> 0.05) higher than compared with TB patients. Also there is a significant increase in APTT of TB patients (P<0.05) when compared with HIV patients. APTT of HIV-TB Co-¬infected subjects were not significantly elevated when compared with HIV and TB patients (P> 0.05).
Conclusion: HIV infections have been shown to affect both the Activated partial thromboplastin time and prothrombin time. There is significant alteration in coagulation parameters (particularly PT and APTT) on tuberculosis, lesions as a result of the mycobacterial infection can induce pro- coagulant tissue factor expression which can lead to coagulation defects.
Three‐dimensional deep neural network for automatic delineation of cervical cancer in planning computed tomography images
Authors: Ding Yi , Chen Zhiran , Wang Ziqi , Wang Xiaohong , Hu Desheng , Ma Pingping , Ma Chi , Wei Wei , Li Xiangbin , Xue Xudong , Wang Xiao 
Published: February 2022

AbstractPurposeRadiation therapy is an essential treatment modality for cervical cancer, while accurate and efficient segmentation methods are needed to improve the workflow. In this study, a three‐dimensional V‐net model is proposed to automatically segment clinical target volume (CTV) and organs at risk (OARs), and to provide prospective guidance for low lose area.Material and methodsA total of 130 CT datasets were included. Ninety cases were randomly selected as the training data, with 10 cases used as the validation data, and the remaining 30 cases as testing data. The V‐net model was implemented with Tensorflow package to segment the CTV and OARs, as well as regions of 5 Gy, 10 Gy, 15 Gy, and 20 Gy isodose lines covered. The auto‐segmentation by V‐net was compared to auto‐segmentation by U‐net. Four representative parameters were calculated to evaluate the accuracy of the delineation, including Dice similarity coefficients (DSC), Jaccard index (JI), average surface distance (ASD), and Hausdorff distance (HD).ResultsThe V‐net and U‐net achieved the average DSC value for CTV of 0.85 and 0.83, average JI values of 0.77 and 0.75, average ASD values of 2.58 and 2.26, average HD of 11.2 and 10.08, respectively. As for the OARs, the performance of the V‐net model in the colon was significantly better than the U‐net model (p = 0.046), and the performance in the kidney, bladder, femoral head, and pelvic bones were comparable to the U‐net model. For prediction of low‐dose areas, the average DSC of the patients’ 5 Gy dose area in the test set were 0.88 and 0.83, for V‐net and U‐net, respectively.ConclusionsIt is feasible to use the V‐Net model to automatically segment cervical cancer CTV and OARs to achieve a more efficient radiotherapy workflow. In the delineation of most target areas and OARs, the performance of V‐net is better than U‐net. It also offers advantages with its feature of predicting the low‐dose area prospectively before radiation therapy (RT).
Engineered Delivery of Dental Stem‐Cell‐Derived Extracellular Vesicles for Periodontal Tissue Regeneration
Authors: Zarubova Jana , Hasani‐Sadrabadi Mohammad Mahdi , Dashtimoghadam Erfan , Zhang Xuexiang , Ansari Sahar , Li Song , Moshaverinia Alireza 
Published: February 2022

AbstractPeriodontal disease begins as an inflammatory response to a bacterial biofilm deposited around the teeth, which over time leads to the destruction of tooth‐supporting structures and consequently tooth loss. Conventional treatment strategies show limited efficacy in promoting regeneration of damaged periodontal tissues. Here, a delivery platform is developed for small extracellular vesicles (sEVs) derived from gingival mesenchymal stem cells (GMSCs) to treat periodontitis. EVs can achieve comparable therapeutic effects to their cells of origin. However, the short half‐lives of EVs after their administration along with their rapid diffusion away from the delivery site necessitate frequent administration to achieve therapeutic benefits. To address these issues, “dual delivery” microparticles are engineered enabling microenvironment‐sensitive release of EVs by metalloproteinases at the affected site along with antibiotics to suppress bacterial biofilm growth. GMSC sEVs are able to decrease the secretion of pro‐inflammatory cytokines by monocytes/macrophages and T cells, suppress T‐cell activation, and induce the formation of T regulatory cells (Tregs) in vitro and in a rat model of periodontal disease. One‐time administration of immunomodulatory GMSC sEV‐decorated microparticles leads to a significant improvement in regeneration of the damaged periodontal tissue. This approach will have potential clinical applications in the regeneration of a variety of tissues.
Hydrogel Transformed from Nanoparticles for Prevention of Tissue Injury and Treatment of Inflammatory Diseases
Authors: Li Zimeng , Li Gang , Xu Jiajia , Li Chenwen , Han Songling , Zhang Chunfan , Wu Peng , Lin Yongyao , Wang Chenping , Zhang Jianxiang , Li Xiaodong 
Published: February 2022

AbstractFunctional hydrogels responsive to physiological and pathological signals have extensive biomedical applications owing to their multiple advanced attributes. Herein, engineering of functional hydrogels is reported via transformable nanoparticles in response to the physiologically and pathologically acidic microenvironment. These nanoparticles are assembled by a multivalent hydrophobic, pH‐responsive cyclodextrin host material and a multivalent hydrophilic guest macromolecule. Driven by protons, the pH‐responsive host–guest nanoparticles can be transformed into hydrogel, resulting from proton‐triggered hydrolysis of the host material, generation of a hydrophilic multivalent host compound, and simultaneously enhanced inclusion interactions between host and guest molecules. By in situ forming a hydrogel barrier, the orally delivered transformable nanoparticles protect mice from ethanol‐ or drug‐induced gastric injury. In addition, this type of nanoparticles can serve as responsive and transformable nanovehicles for therapeutic agents to achieve triggerable and sustained drug delivery, thereby effectively treating typical inflammatory diseases, including periodontitis and arthritis in rats. With combined advantages of nanoparticles and hydrogels, together with their good in vivo safety, the engineered transformable nanoparticles hold great promise in tissue injury protection and site‐specific/local delivery of molecular and cellular therapeutic agents.
A Fibrin Site‐Specific Nanoprobe for Imaging Fibrin‐Rich Thrombi and Preventing Thrombus Formation in Venous Vessels
Authors: Zhong Yixin , Ye Man , Huang Liandi , Hu Liu , Li Fangzhou , Ni Qiankun , Zhong Jie , Wu Hongyun , Xu Fengfei , Xu Jie , He Xiaojing , Wang Zhigang , Ran Haitao , Wu Yunzhu , Guo Dajing , Liang Xing‐Jie 
Published: February 2022

AbstractVenous thromboembolism (VTE) is a prevalent public health issue worldwide. Before treatment, spatiotemporally accurate thrombus detection is essential. However, with the currently available imaging technologies, this is challenging. Herein, the development of a novel fibrin‐specific nanoprobe (NP) based on the conjugation of poly(lactic‐co‐glycolic acid) with the pentapeptide Cys−Arg−Glu−Lys−Ala (CREKA) for selective and semiquantitative imaging in vivo is presented. By integrating Fe3O4 and NIR fluorochrome (IR780), the NP can function as a highly sensitive sensor for the direct analysis of thrombi in vivo. The fibrin‐specific NP distinguishes fibrin‐rich thrombi from collagen‐rich or erythrocyte‐rich thrombi, which can be beneficial for future individually tailored therapeutic strategy. Furthermore, loading NPs with the ketotifen fumarate results in mast cell degranulation inhibition, and hence, NPs can prevent thrombosis without the risk of excessive bleeding. Thus, the use of fibrin‐specific NPs may serve as a safe alternative approach for the detection and prevention of VTEs in susceptible populations in the future.
Trends in Nanophotonics‐Enabled Optofluidic Biosensors
Authors: Wang Juan , Maier Stefan A. , Tittl Andreas 
Published: February 2022

AbstractOptofluidic sensors integrate photonics with micro/nanofluidics to realize compact devices for the label‐free detection of molecules and the real‐time monitoring of dynamic surface binding events with high specificity, ultrahigh sensitivity, low detection limit, and multiplexing capability. Nanophotonic structures composed of metallic and/or dielectric building blocks excel at focusing light into ultrasmall volumes, creating enhanced electromagnetic near‐fields ideal for amplifying the molecular signal readout. Furthermore, fluidic control on small length scales enables precise tailoring of the spatial overlap between the electromagnetic hotspots and the analytes, boosting light‐matter interaction, and can be utilized to integrate advanced functionalities for the pre‐treatment of samples in real‐world‐use cases, such as purification, separation, or dilution. In this review, the authors highlight current trends in nanophotonics‐enabled optofluidic biosensors for applications in the life sciences while providing a detailed perspective on how these approaches can synergistically amplify the optical signal readout and achieve real‐time dynamic monitoring, which is crucial in biomedical assays and clinical diagnostics.
Palladium‐Catalyzed Chemoselective Borylation of (Poly)halogenated Aryl Triflates and Their Application in Consecutive Reactions
Authors: Ng Shan Shan , Chen Zicong , Yuen On Ying , So Chau Ming 
Published: February 2022

AbstractThis study reports the palladium‐catalyzed chemoselective borylation of (poly)halogenated aryl triflates with a reactivity order of C−Cl>C−OTf. A catalyst system comprising Pd(OAc)2 and SelectPhos (L1) enables a reaction with high reactivity and chemoselectivity. The consecutively chemoselective borylation reaction followed by the chemoselective intermolecular Suzuki‐Miyaura reaction can be performed using a one‐pot two‐step approach to synthesize unsymmetrical biaryl compounds containing the triflate moiety. The reaction can be scaled up to the gram scale without diminishing the yield and chemoselectivity.magnified image
Revealing the Design Principles of Ni‐Rich Cathodes for All‐Solid‐State Batteries
Authors: Jiang Wei , Zhu Xinxin , Huang Renzhi , Zhao Shu , Fan Xinming , Ling Min , Liang Chengdu , Wang Liguang 
Published: February 2022

AbstractElectrode microstructure is one of the primary factors determining the electrochemical properties of all‐solid‐state batteries (ASSBs). However, the key principles of electrode design to realize fast ionic/electronic transport pathways are not well elucidated. Herein, the correlation between electrode microstructure and electrochemical behaviors is studied through different electrode design in terms of energy‐density‐related electrode composition and Ni‐rich layered oxides particle size distribution. A positive relation between particle size and mass fraction in electrode design is revealed, that is, as the particle size increases, more cathode materials are required to enable the fast reaction kinetics. The well interconnected active particles and solid electrolytes construct the highly percolated ionic/electronic transfer networks, which determines the overall electrochemical properties. The findings in this work give a new insight into electrode design to promote the development of high‐energy and high‐power ASSBs.
MOF‐801 polycrystalline membrane with sub‐10 nm polymeric assembly layer for ion sieving and flow battery storage
Authors: Cao Hongyan , Xia Yu , Lu Yuqin , Wu Yulin , Xia Yongsheng , Hou Xiaoxuan , Wang Yixing , Liu Gongping , Huang Kang , Xu Zhi 
Published: February 2022

AbstractMolecular sieving metal–organic framework (MOF) polycrystalline membranes have great potential for ion sieving and are desirable as efficient separators for devices of energy storage such as flow battery. Herein, we report a continuous MOF‐801 polycrystalline membrane with an ultrathin polymeric assembly layer (less than 10 nm) for the vanadium flow battery (VFB). Owing to the precise sub‐nanometer sieving pores and abundant H‐bond networks in MOF‐801 frameworks, the membrane exhibited better H/V selectivity (up to 194) and conductivity (about 0.028 S/cm) than commercial Nafion‐117 membrane (H/V selectivity: ~9.5, conductivity: 0.017 S/cm). VFB results revealed that a cell with above MOF polycrystalline membrane showed high coulombic efficiency (CE: 96.1%) and excellent energy efficiency (EE: 83.2%) at 20 mA/cm2, which was much comparable to Nafion membrane. This work demonstrates that MOF polycrystalline membrane is a promising candidate as ion sieving membrane for energy technique.
A techno‐economic analysis framework for intensified modular systems
Authors: Gazzaneo Vitor , Watson Madelynn , Ramsayer Carlie B. , Kilwein Zachary A. , Alves Victor , Lima Fernando V. 
Published: February 2022

AbstractIn this work, a novel systematic techno‐economic analysis framework is proposed for costing intensified modular systems. Conventional costing techniques are extended to allow estimation of capital and operating costs of modular units. Economy of learning concepts are included to consider the effect of experience curves on purchase costs. Profitability measures are scaled with respect to production of a chemical of interest for comparison with plants of traditional scale. In the developed framework, a base case scenario is analyzed to identify the relevance of the economy of learning and cost parameters that are yet to be established for modular projects that will be deployed. Then, a sensitivity analysis step is conducted to define changes in relevant variables that benefit the construction of modular systems. In a final step, scenarios in which the modular technology presents break‐even and further reduction in cost are identified. A process model for a modular hydrogen unit is developed and used for demonstration of the proposed framework. In this application, process synthesis is carried out, including operability analysis for selection of feasible operating conditions. A comparison with a benchmark conventional steam methane reforming plant shows that the modular hydrogen unit can benefit from the economy of learning. A synthesized flowsheet for a modular steam methane reforming plant is used to map the decrease in natural gas price that must be needed for the plant to break even when compared to traditional technologies. Scenarios in which the natural gas price is low allow break‐even cost for both individual hydrogen units and the assembled modular plant. For such break‐even cases, the economy of learning must produce a reduction of 40% or less in capital cost when the natural gas price is under 0.02 US$/Sm3. This result suggests that the synthesized modular hydrogen process has potential to be economically feasible under these conditions. The developed tools can thus be used to accelerate the deployment and manufacturing of standardized modular energy systems.
New 1,3,4‐oxadiazoles linked with the 1,2,3‐triazole moiety as antiproliferative agents targeting the EGFR tyrosine kinase
Authors: Mahmoud Mohamed A. , Mohammed Anber F. , Salem Ola I. A. , Gomaa Hesham A. M. , Youssif Bahaa G. M. 
Published: February 2022

AbstractA series of 1,3,4‐oxadiazole‐1,2,3‐triazole hybrids bearing different pharmacophoric moieties has been designed and synthesized. Their antiproliferative activity was evaluated against four human cancer cell lines (Panc‐1, MCF‐7, HT‐29, and A‐549) using the MTT (3‐(4,5‐dimethylthiazol‐2‐yl)−2,5‐diphenyltetrazolium bromide) assay. The preliminary activity test displayed that the most active compounds, 6d, 6e, and 8a–e, suppressed cancer cell growth (GI50 = 0.23–2.00 µM) comparably to erlotinib (GI50 = 0.06 µM). Compounds 6d, 6e, and 8a–e inhibited the epidermal growth factor receptor tyrosine kinase (EGFR‐TK) at IC50 = 0.11–0.73 µM, compared to erlotinib (IC50 = 0.08 ± 0.04 µM). The apoptotic mechanism revealed that the most active hybrid 8d induced expression levels of caspase‐3, caspase‐9, and cytochrome‐c in the human cancer cell line Panc‐1 by 7.80‐, 19.30‐, and 13‐fold higher than doxorubicin. Also, 8d increased the Bax level by 40‐fold than doxorubicin, along with decreasing Bcl‐2 levels by 6.3‐fold. Cell cycle analysis after treatment of Panc‐1 cells with hybrid 8d revealed a high proportion of cell accumulation (41.53%) in the pre‐G1 phase, indicating cell cycle arrest at the G1 transition. Computational docking of the 8d and 8e hybrids with the EGFR binding site revealed their ability to bind with EGFR similar to erlotinib. Finally, in silico absorption, distribution, metabolism, and excretion/pharmacokinetic studies for the most active hybrids are discussed.
Preparation and luminescence properties of KLaSiO4:Tb3+ phosphors
Authors: Ma Wenjing , Wang Xigui 
Published: February 2022

AbstractKLaSiO4:Tb3+ phosphors were synthesized using the sol–gel method. The structure and luminescence properties of the materials were characterized using X‐ray diffraction, Fourier transform infrared (FTIR) spectroscopy, thermogravimetry–differential thermal analysis, fluorescence spectra and calculated Commission Internationale de l'éclairage coordinates. The results showed that the material had a hexagonal structure, and that doping of Tb3+ did not change the crystal structure of KLaSiO4. FTIR spectroscopy confirmed the existence of stretching vibrations of Si–O, bending vibrations of Si–O–Si, and asymmetric tensile vibrations of Si–O in KLaSiO4. The excitation spectrum of the sample consisted of 4f7→5d1 broadband absorption and the characteristic excitation peak of Tb3+, the excitation peak at 232 nm belongs to the spin allowed 7FJ→7DJ transition of Tb3+, the excitation peak at 268 nm belongs to the spin forbidden 7FJ→9DJ transition of Tb3+, and the absorption band of 7FJ→7DJ transition is split. Under excitation at 232 nm, the emission peak of the sample was composed of the 5D4→7FJ (J = 6, 5, 4, 3) energy level transition of Tb3+. The highest emission peak is located at 543 nm, which belongs to the 5D4→7F5 transition and emits green light. Concentration quenching occurred when the Tb3+ doping concentration was greater than 1% mol, the quenching mechanism was an electric dipole–electric dipole action. When the ratio of citric acid to total metal ions was 1:1 and the annealing temperature was 800°C, the surface defects of the phosphors were greatly reduced, the quenching effect was reduced, and the luminous intensity reached the maximum.