bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic remodelling of Fxyd1 promoters in developing heart and brain tissues
Authors: Cuomo Mariella , Florio Ermanno , Della Monica Rosa , Costabile Davide , Buonaiuto Michela , Di Risi Teodolinda , De Riso Giulia , Sarnataro Antonella , Cocozza Sergio , Visconti Roberta , Chiariotti Lorenzo 
Published: April 2022

AbstractFXYD1 is a key protein controlling ion channel transport. FXYD1 exerts its function by regulating Na+/K+-ATPase activity, mainly in brain and cardiac tissues. Alterations of the expression level of the FXYD1 protein cause diastolic dysfunction and arrhythmias in heart and decreased neuronal dendritic tree and spine formation in brain. Moreover, FXYD1, a target of MeCP2, plays a crucial role in the pathogenesis of the Rett syndrome, a neurodevelopmental disorder. Thus, the amount of FXYD1 must be strictly controlled in a tissue specific manner and, likely, during development. Epigenetic modifications, particularly DNA methylation, represent the major candidate mechanism that may regulate Fxyd1 expression. In the present study, we performed a comprehensive DNA methylation analysis and mRNA expression level measurement of the two Fxyd1 transcripts, Fxyd1a and Fxyd1b, in brain and heart tissues during mouse development. We found that DNA methylation at Fxyd1a increased during brain development and decreased during heart development along with coherent changes in mRNA expression levels. We also applied ultra-deep methylation analysis to detect cell to cell methylation differences and to identify possible distinct methylation profile (epialleles) distribution between heart and brain and in different developmental stages. Our data indicate that the expression of Fxyd1 transcript isoforms inversely correlates with DNA methylation in developing brain and cardiac tissues suggesting the existence of a temporal-specific epigenetic program. Moreover, we identified a clear remodeling of epiallele profiles which were distinctive for single developmental stage both in brain and heart tissues.
Efficacy of high-flow nasal oxygenation against peri- and post-procedural hypoxemia in patients with obesity: a meta-analysis of randomized controlled trials
Authors: Hung Kuo-Chuan , Ko Ching-Chung , Chang Po-Chih , Wang Kuei-Fen , Teng I.-Chia , Lin Chien-Hung , Huang Ping-Wen , Sun Cheuk-Kwan 
Published: April 2022

AbstractThis meta-analysis aimed at investigating the efficacy of high-flow nasal oxygenation (HFNO) against hypoxemia in patients with obesity compared with conventional oxygenation therapy and non-invasive ventilation. Databases were searched from inception to August 2021. Studies involving peri- or post-procedural use of HFNO were included. The primary outcome was risk of hypoxemia, while the secondary outcomes included status of oxygenation and carbon dioxide elimination. Ten randomized controlled trials (RCTs) were included. We found that HFNO prolonged the safe apnea time at induction compared to control group [mean difference (MD) = 73.88 s, p = 0.0004; 2 RCTs] with no difference in risk of peri-procedural hypoxemia [relative risk (RR) = 0.91, p = 0.64; 4 RCTs], minimum SpO2 (MD = 0.09%, p = 0.95; 4 RCTs), PaO2 (MD = − 8.13 mmHg, p = 0.86; 3 RCTs), PaCO2 (MD = − 6.71%, p = 0.2; 2 RCTs), EtCO2 (MD = − 0.28 mmHg, p = 0.8; 4 RCTs) between the two groups. HFNO also did not improve postprocedural PaO2/FiO2 ratio (MD = 41.76, p = 0.58; 2 RCTs) and PaCO2 (MD = − 2.68 mmHg, p = 0.07; 2 RCTs). This meta-analysis demonstrated that the use of HFNO may be associated with a longer safe apnea time without beneficial impact on the risk of hypoxemia, oxygenation, and CO2 elimination in patients with obesity. The limited number of trials warranted further large-scale studies to support our findings.
Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective
Authors: Cansby Emmelie , Kumari Sima , Caputo Mara , Xia Ying , Porosk Rando , Robinson Jonathan , Wang Hao , Olsson Britt-Marie , Vallin Josefine , Grantham Julie , Soomets Ursel , Svensson L. Thomas , Sihlbom Carina , Marschall Hanns-Ulrich , Edsfeldt Andreas , Goncalves Isabel , Mahlapuu Margit 
Published: April 2022

AbstractRecent studies highlight the importance of lipotoxic damage in aortic cells as the major pathogenetic contributor to atherosclerotic disease. Since the STE20-type kinase STK25 has been shown to exacerbate ectopic lipid storage and associated cell injury in several metabolic organs, we here investigate its role in the main cell types of vasculature. We depleted STK25 by small interfering RNA in human aortic endothelial and smooth muscle cells exposed to oleic acid and oxidized LDL. In both cell types, the silencing of STK25 reduces lipid accumulation and suppresses activation of inflammatory and fibrotic pathways as well as lowering oxidative and endoplasmic reticulum stress. Notably, in smooth muscle cells, STK25 inactivation hinders the shift from a contractile to a synthetic phenotype. Together, we provide several lines of evidence that antagonizing STK25 signaling in human aortic endothelial and smooth muscle cells is atheroprotective, highlighting this kinase as a new potential therapeutic target for atherosclerotic disease.
MFG-E8 promotes osteogenic transdifferentiation of smooth muscle cells and vascular calcification by regulating TGF-β1 signaling
Authors: Chiang Hou-Yu , Chu Pao-Hsien , Chen Shao-Chi , Lee Ting-Hein 
Published: April 2022

AbstractVascular calcification occurs in arterial aging, atherosclerosis, diabetes mellitus, and chronic kidney disease. Transforming growth factor-β1 (TGF-β1) is a key modulator driving the osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs), leading to vascular calcification. We hypothesize that milk fat globule–epidermal growth factor 8 (MFG-E8), a glycoprotein expressed in VSMCs, promotes the osteogenic transdifferentiation of VSMCs through the activation of TGF-β1-mediated signaling. We observe that the genetic deletion of MFG-E8 prevents calcium chloride-induced vascular calcification in common carotid arteries (CCAs). The exogenous application of MFG-E8 to aged CCAs promotes arterial wall calcification. MFG-E8-deficient cultured VSMCs exhibit decreased biomineralization and phenotypic transformation to osteoblast-like cells in response to osteogenic medium. MFG-E8 promotes β1 integrin–dependent MMP2 expression, causing TGF-β1 activation and subsequent VSMC osteogenic transdifferentiation and biomineralization. Thus, the established molecular link between MFG-E8 and vascular calcification suggests that MFG-E8 can be therapeutically targeted to mitigate vascular calcification.
Exome sequencing of families from Ghana reveals known and candidate hearing impairment genes
Authors: Wonkam Ambroise , Adadey Samuel Mawuli , Schrauwen Isabelle , Aboagye Elvis Twumasi , Wonkam-Tingang Edmond , Esoh Kevin , Popel Kalinka , Manyisa Noluthando , Jonas Mario , deKock Carmen , Nembaware Victoria , Cornejo Sanchez Diana M. , Bharadwaj Thashi , Nasir Abdul , Everard Jenna L. , Kadlubowska Magda K. , Nouel-Saied Liz M. , Acharya Anushree , Quaye Osbourne , Amedofu Geoffrey K. , Awandare Gordon A. , Leal Suzanne M. 
Published: April 2022

AbstractWe investigated hearing impairment (HI) in 51 families from Ghana with at least two affected members that were negative forGJB2pathogenic variants. DNA samples from 184 family members underwent whole-exome sequencing (WES). Variants were found in 14 known non-syndromic HI (NSHI) genes [26/51 (51.0%) families], five genes that can underlie either syndromic HI or NSHI [13/51 (25.5%)], and one syndromic HI gene [1/51 (2.0%)]. Variants inCDH23andMYO15Acontributed the most to HI [31.4% (16/51 families)]. ForDSPP, an autosomal recessive mode of inheritance was detected. Post-lingual expression was observed for a family segregating aMARVELD2variant. To our knowledge, seven novel candidate HI genes were identified (13.7%), with six associated with NSHI (INPP4B,CCDC141, MYO19, DNAH11, POTEI, andSOX9); and one (PAX8) with Waardenburg syndrome.MYO19andDNAH11were replicated in unrelated Ghanaian probands. Six of the novel genes were expressed in mouse inner ear. It is known thatPax8-/-mice do not respond to sound, and depletion of Sox9 resulted in defective vestibular structures and abnormal utricle development. Most variants (48/60; 80.0%) have not previously been associated with HI. Identifying seven candidate genes in this study emphasizes the potential of novel HI genes discovery in Africa.
Molecular dynamics simulations of the evaporation of hydrated ions from aqueous solution
Authors: Loche Philip , Bonthuis Douwe J. , Netz Roland R. 
Published: April 2022

AbstractAlthough important for atmospheric processes and gas-phase catalysis, very little is known about the hydration state of ions in the vapor phase. Here we study the evaporation energetics and kinetics of a chloride ion from liquid water by molecular dynamics simulations. As chloride permeates the interface, a water finger forms and breaks at a chloride separation of ≈ 2.8 nm from the Gibbs dividing surface. For larger separations from the interface, about 7 water molecules are estimated to stay bound to chloride in saturated water vapor, as corroborated by continuum dielectrics and statistical mechanics models. This ion hydration significantly reduces the free-energy barrier for evaporation. The effective chloride diffusivity in the transition state is found to be about 6 times higher than in bulk, which reflects the highly mobile hydration dynamics as the water finger breaks. Both effects significantly increase the chloride evaporation flux from the quiescent interface of an electrolyte solution, which is predicted from reaction kinetic theory.
Optimizing higher-order network topology for synchronization of coupled phase oscillators
Authors: Tang Ying , Shi Dinghua , Lü Linyuan 
Published: April 2022

AbstractNetworks in nature have complex interactions among agents. One significant phenomenon induced by interactions is synchronization of coupled agents, and the interactive network topology can be tuned to optimize synchronization. Previous studies showed that the optimized conventional network with pairwise interactions favors a homogeneous degree distribution of nodes for undirected interactions, and is always structurally asymmetric for directed interactions. However, the optimal control on synchronization for prevailing higher-order interactions is less explored. Here, by considering the higher-order interactions in a hypergraph and the Kuramoto model with 2-hyperlink interactions, we find that the network topology with optimized synchronizability may have distinct properties. For undirected interactions, optimized networks with 2-hyperlink interactions by simulated annealing tend to become homogeneous in the nodes’ generalized degree. We further rigorously demonstrate that for directed interactions, the structural symmetry can be preserved in the optimally synchronizable network with 2-hyperlink interactions. The results suggest that controlling the network topology of higher-order interactions leads to synchronization phenomena beyond pairwise interactions.
Annexin A1 treatment prevents the evolution to fibrosis of experimental nonalcoholic steatohepatitis
Authors: Gadipudi Laila Lavanya , Ramavath Naresh Naik , Provera Alessia , Reutelingsperger Chris , Albano Emanuele , Perretti Mauro , Sutti Salvatore 
Published: April 2022

Abstract
Annexin A1 (AnxA1) is an important effector in the resolution of inflammation which is involved in modulating hepatic inflammation in nonalcoholic steatohepatitis (NASH). In the present study, we have investigated the possible effects of treatment with AnxA1 for counteracting the progression of experimental NASH.
NASH was induced in C57BL/6 mice by feeding methionine–choline deficient (MCD) or Western diets (WDs) and the animals were treated for 4–6 weeks with human recombinant AnxA1 (hrAnxA1; 1 µg, daily IP) or saline once NASH was established.
In both experimental models, treatment with hrAnxA1 improved parenchymal injury and lobular inflammation without interfering with the extension of steatosis. Furthermore, administration of hrAnxA1 significantly attenuated the hepatic expression of α1-procollagen and TGF-β1 and reduced collagen deposition, as evaluated by collagen Sirius Red staining. Flow cytometry and immunohistochemistry showed that hrAnxA1 did not affect the liver recruitment of macrophages, but strongly interfered with the formation of crown-like macrophage aggregates and reduced their capacity of producing pro-fibrogenic mediators like osteopontin (OPN) and galectin-3 (Gal-3). This effect was related to an interference with the acquisition of a specific macrophage phenotype characterized by the expression of the Triggering Receptor Expressed on Myeloid cells 2 (TREM-2), CD9 and CD206, previously associated with NASH evolution to cirrhosis.
Collectively, these results indicate that, beside ameliorating hepatic inflammation, AnxA1 is specifically effective in preventing NASH-associated fibrosis by interfering with macrophage pro-fibrogenic features. Such a novel function of AnxA1 gives the rationale for the development of AnxA1 analogs for the therapeutic control of NASH evolution.
Transient surface charge of SF6‐filled direct current gas‐insulated transmission lines insulator under thermal‐electric coupled fields: effects of ambient temperature, load current and gas pressure
Authors: Li Xiaolong , Han Songling , Wang Wen , Geng Zhenxin , Lin Xin 
Published: April 2022

AbstractThe transient surface charge characteristics of a basin‐type insulator in horizontally installed SF6‐filled direct current gas‐insulated transmission lines (DC‐GIL) are investigated based on a three‐dimension (3D) simulation model under thermal‐electric coupled stress. The influence of ambient temperature, load current and gas pressure on the surface charge and electric field characteristics is investigated. Simulation results indicate that the surface charge accumulation process is accelerated due to the promotion of bulk conduction under high ambient temperature, high load current and low gas pressure. The transient field variation is accelerated as the consequence. The highest field strength on the convex surface increases and moves towards the enclosure with increasing time. With increasing load current, the field strength increases due to the influence of charge accumulation and temperature‐dependent conductivity of the insulator. It is revealed that the transient surface charge under coupled fields should be focused when dealing with the insulation characteristics of DC‐GIL insulators during long‐term operation. The mentioned operating conditions should be paid attention. This method introduced in this study can help to evaluate the 3D transient charge and field characteristics under various conditions.
Manganese-Catalyzed Synthesis of Imines from Primary Alcohols and (Hetero)Aromatic Amines
Authors: Raydan Daniel , Friães Sofia , Royo Beatriz , Marques M. Manuel B. , Viduedo Nuno , Santos A. Sofia , Gomes Clara S. B. 
Published: April 2022

AbstractHerein, we describe the synthesis of a wide variety of imines through oxidative coupling of alcohols and aromatic amines catalyzed by Mn complexes bearing N^N triazole ligands. A wide variety of imines in excellent yields (up to 99%) have been prepared. Mn-based catalysts proved to be highly efficient and versatile, allowing for the first time the preparation of several imines containing N-based heterocycles.
In Vitro and In Silico Evaluation of ACE2 and LOX Inhibitory Activity of Origanum Essential Oils and Carvacrol
Authors: Demirci Fatih , Teralı Kerem , Karadağ Ayşe Esra , Biltekin Sevde Nur , Ak Sakallı Ezgi , Demirci Betül , Koşar Müberra , Başer K. Hüsnü Can 
Published: April 2022

Abstract
Origanum spp. are used both for culinary purposes and for their biological activities. In this study, commercial Origanum majorana, Origanum minutiflorum, Origanum vulgare,
and Origanum onites essential oils and their prominent constituent carvacrol were evaluated for their in vitro and in silico angiotensin-converting enzyme 2 and
lipoxygenase enzyme inhibitory potentials. The essential oils were analysed by gas chromatography-flame ionisation detection and gas chromatography-mass spectrometry, where carvacrol was
identified as the major component (62 – 81%), confirming the quality. In vitro enzyme inhibition assays were conducted both with the essential oils (20 µg/mL) and with carvacrol
(5 µg/mL). The comparative values of angiotensin-converting enzyme 2 percent inhibition for O. majorana, O. minutiflorum, O. vulgare, and O. onites essential oils were
determined as 85.5, 79.1, 74.3, and 42.8%, respectively. As a result of the enzyme assays, carvacrol showed 90.7% in vitro angiotensin-converting enzyme 2 inhibitory activity. The
in vitro lipoxygenase inhibition of the essential oils (in the same order) was 89.4, 78.9, 81.1, and 73.5%, respectively, where carvacrol showed 74.8% inhibition. In addition,
protein–ligand docking and interaction profiling was used to gain structural and mechanistic insights into the angiotensin-converting enzyme 2 and lipoxygenase inhibitory potentials of major
Origanum essential oil constituents. The in silico findings agreed with the significant enzyme inhibition activity observed in vitro. Further in vivo studies are
suggested to confirm the safety and efficacy of the oils.
Synthesis of Cyclopropyl Pinacol Boronic Esters from Dibromo­cyclopropanes
Authors: Neouchy Zeina , Hullaert Jan , Verhoeven Jonas , Meerpoel Lieven , Thuring Jan-Willem , Verniest Guido , Winne Johan 
Published: April 2022

AbstractThe synthesis of cyclopropyl pinacol boronic esters from dibromocyclopropanes via Matteson–Pasto rearrangement is reported. The method is readily scalable and shows limited levels of stereoinduction, with a selectivity that is in part complementary to that observed in existing stereoselective borylcyclopropanation strategies. The method can be used to rapidly access borylcyclopropanes as interesting building blocks for diversely functionalized cyclopropanes.
Management of Atopic Dermatitis with Individualised Homoeopathic Treatment
Authors: Sanyal Srimonti , Bhaumik Himadri , Goswami Pritam 
Published: April 2022

AbstractSkin being an important protective organ of body has its own functional and cosmetic values. Atopic dermatitis (AD) is a disease comprising of heterogenous skin manifestations with intense itching. AD affects people of all age groups and ethnicities and has psychosocial impact on the patient. Being the leading cause of global burden of skin disease, this disease also enhances the chances of developing food allergy, allergic asthma etc. Moreover, due to poor hygiene and decreased protective barrier function, AD often gets infected by certain pathogens (Staphylococcus aureus, Malassezia, Molluscum etc.), as a result of which patient suffers from life-threatening complications. Absence of specific diagnostic test with gradual escalation in the disease burden makes it one of the chief concerns for the health care workers. For dermatological diseases, homoeopathy stands out as a prime choice for many patients. In this case, a 72-year-old male patient presented with symptoms of dry, itchy eruptions on right hand for the last 2 years. He also developed similar itching eruptions in and around navel. The patient was clinically diagnosed with AD and treated with individualised homoeopathic treatment. Initially, he was prescribed Tuberculinum which improved the patient and finally he was cured by Sulphur.
Endovascular Procedure in Patient with Ruptured Abdominal Aortic Aneurysm after High-Risk Early Percutaneous Coronary Intervention
Authors: Taofan Taofan , Adiarto Suko , Indriani Suci , Rezeki Arindya , Oepangat Emanoel , Santoso Rony Marethianto 
Published: April 2022

AbstractThe aim of the study is to present the success of an endovascular procedure for ruptured abdominal aortic aneurysm (AAA) patient with high-risk non-ST elevation myocardial infarct (NSTEMI) after early percutaneous coronary intervention (PCI). A 56-year-old man came to our emergency room with a history of early PCI in the previous hospital and received dual antiplatelet therapy (DAPT). His COVID-19 test result was unknown. This patient was then being re-examined and was diagnosed with ruptured AAA. Despite his pending COVID-19 laboratory results, we decided to perform an urgent endovascular aortic repair (EVAR) in this patient, considering his DAPT consumption history. The procedure was successful and the patient's condition after EVAR showed improvements. In patients with ruptured AAA with high-risk NSTEMI who just underwent early PCI and recently received DAPT, endovascular procedure can be considered as the treatment of choice since open surgery repair is contraindicated.
Minimally Invasive Transpedicular Screw Fixation: Review of 152 Cases in a Single Institution. Steep or Shallow Learning Curve?
Authors: Gabrovsky Nikolay , Ilkov Petar , Laleva Maria , Iliev Cvetoslav , Gabrovsky Stefan 
Published: April 2022

Abstract
Background In this study, we analyze our institutional experience and personal impressions using minimally invasive spine surgery (MISS) to describe our learning curve and how experience influenced different parameters of the surgical procedure.
Methods The study was conducted prospectively and included the first consecutive 152 patients treated with MISS techniques. Patient demographics, surgical data, length of hospital stay, and clinical outcome were reviewed. The cohort was divided into consecutive quarters. Comparison between the quarters and timeline analysis were made to assess the learning curve.
Results Only percutaneous transpedicular screw fixation was performed in 65 cases, minimally invasive transforaminal lumbar interbody fusion (MI-TLIF) in 70 cases, and vertebral body replacement in 4 cases. The average blood loss was 113.3, 115, 106.6, and 107.1 mL for each quarter. The average operative time was 155.0, 143.2, 134.5, and 133.8 minutes for the four quarters, whereas the average radiation exposure time was 105.4, 85.3, 46.2, and 45.2 seconds. Differences in the operative time and radiation exposure time between the first to third and the first to fourth quarters were statistically significant.
Conclusions Some advantages of MISS techniques could be observed with the very first cases and were not related significantly with the surgeon's experience with MISS. With the acquisition of more experience, some disadvantages of MISS techniques such as longer operative time and longer X-ray exposure can be substantially reduced. Surgical experience, familiarity of the team with the MISS instrumentation, and good patient selection are crucial for achieving all the benefits of MISS.