bluuebunny/crossref_metadata_2025_split huggingface dataset:
Terahertz pulse-driven collective mode in the nematic superconducting state of Ba1−xKxFe2As2
Authors: Grasset Romain , Katsumi Kota , Massat Pierre , Wen Hai-Hu , Chen Xian-Hui , Gallais Yann , Shimano Ryo 
Published: January 2022

AbstractWe investigate the collective mode response of the iron-based superconductor Ba1−xKxFe2As2 using intense terahertz (THz) light. In the superconducting state a THz Kerr signal is observed and assigned to nonlinear THz coupling to superconducting degrees of freedom. The polarization dependence of the THz Kerr signal is remarkably sensitive to the coexistence of a nematic order. In the absence of nematic order the C4 symmetric polarization dependence of the THz Kerr signal is consistent with a coupling to the Higgs amplitude mode of the superconducting condensate. In the coexisting nematic and superconducting state the signal becomes purely nematic with a vanishing C4 symmetric component, signaling the emergence of a superconducting collective mode activated by nematicity.
Structure of UBE2K–Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension
Authors: Nakasone Mark A. , Majorek Karolina A. , Gabrielsen Mads , Sibbet Gary J. , Smith Brian O. , Huang Danny T. 
Published: January 2022

AbstractUbiquitin (Ub) chain types govern distinct biological processes. K48-linked polyUb chains target substrates for proteasomal degradation, but the mechanism of Ub chain synthesis remains elusive due to the transient nature of Ub handover. Here, we present the structure of a chemically trapped complex of the E2 UBE2K covalently linked to donor Ub and acceptor K48-linked di-Ub, primed for K48-linked Ub chain synthesis by a RING E3. The structure reveals the basis for acceptor Ub recognition by UBE2K active site residues and the C-terminal Ub-associated (UBA) domain, to impart K48-linked Ub specificity and catalysis. Furthermore, the structure unveils multiple Ub-binding surfaces on the UBA domain that allow distinct binding modes for K48- and K63-linked Ub chains. This multivalent Ub-binding feature serves to recruit UBE2K to ubiquitinated substrates to overcome weak acceptor Ub affinity and thereby promote chain elongation. These findings elucidate the mechanism of processive K48-linked polyUb chain formation by UBE2K.
Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
Authors: Laporte Marine H. , Klena Nikolai , Hamel Virginie , Guichard Paul 
Published: January 2022

AbstractCryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy.
A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of Bean pod mottle virus
Authors: Yang Qian-Qian , Zhao Xing-Xing , Wang Dao , Zhang Peng-Jun , Hu Xue-Nan , Wei Shuang , Liu Jing-Yuan , Ye Zi-Hong , Yu Xiao-Ping 
Published: January 2022

AbstractBean pod mottle virus(BPMV) is a destructive virus that causes serious economic losses in many countries every year, highlighting the importance of its effective detection. In this study, we developed a fast reverse transcription-cross-priming amplification (RT-CPA) coupled with lateral flow dipstick (LFD) diagnostic method for BPMV detection. The RT-CPA-LFD assay that targets the coat protein gene of BPMV was highly specific against diagnosing four other common viruses transmitted by soybean seeds, i.e.,Southern bean mosaic virus(SBMV),Tomato ringspot virus (ToRSV),Arabis mosaic virus (ArMV), andTobacco ringspot virus (TRSV). The sensitivities of the real-time fluorescent RT-CPA and the RT-CPA-LFD assay were at least 50 pg/μl and 500 pg/μl, respectively. Despite a compromise in the limit of detection of the RT-CPA method compared with TaqMan-MGB real-time RT-PCR, our results demonstrated a notably better performance in the detection of field samples of BPMV-infested soybean seeds. With the advantages of efficiency and convenience by visual determination, the RT-CPA-LFD assay presents a potential application for the rapid and accurate detection of BPMV in routine tests.
Synergy between an emerging monopartite begomovirus and a DNA-B component
Authors: Ouattara Alassane , Tiendrébéogo Fidèle , Becker Nathalie , Urbino Cica , Thébaud Gaël , Hoareau Murielle , Allibert Agathe , Chiroleu Frédéric , Vernerey Marie-Stéphanie , Traoré Edgar Valentin , Barro Nicolas , Traoré Oumar , Lefeuvre Pierre , Lett Jean-Michel 
Published: January 2022

AbstractIn recent decades, a legion of monopartite begomoviruses transmitted by the whitefly Bemisia tabaci has emerged as serious threats to vegetable crops in Africa. Recent studies in Burkina Faso (West Africa) reported the predominance of pepper yellow vein Mali virus (PepYVMLV) and its frequent association with a previously unknown DNA-B component. To understand the role of this DNA-B component in the emergence of PepYVMLV, we assessed biological traits related to virulence, virus accumulation, location in the tissue and transmission. We demonstrate that the DNA-B component is not required for systemic movement and symptom development of PepYVMLV (non-strict association), but that its association produces more severe symptoms including growth arrest and plant death. The increased virulence is associated with a higher viral DNA accumulation in plant tissues, an increase in the number of contaminated nuclei of the phloem parenchyma and in the transmission rate by B. tabaci. Our results suggest that the association of a DNA-B component with the otherwise monopartite PepYVMLV is a key factor of its emergence.
Lapatinib as first-line treatment for muscle-invasive urothelial carcinoma in dogs
Authors: Maeda Shingo , Sakai Kosei , Kaji Kenjiro , Iio Aki , Nakazawa Maho , Motegi Tomoki , Yonezawa Tomohiro , Momoi Yasuyuki 
Published: January 2022

AbstractEpidermal growth factor receptors 1 and 2 (EGFR and HER2) are frequently overexpressed in various malignancies. Lapatinib is a dual tyrosine kinase inhibitor that inhibits both EGFR and HER2. Although a phase III trial failed to show the survival benefits of lapatinib treatment after first-line chemotherapy in patients with EGFR/HER2-positive metastatic urothelial carcinoma, the efficacy of lapatinib for untreated urothelial carcinoma is not well defined. Here, we describe the therapeutic efficacy of lapatinib as a first-line treatment in a canine model of muscle-invasive urothelial carcinoma. In this non-randomized clinical trial, we compared 44 dogs with naturally occurring urothelial carcinoma who received lapatinib and piroxicam, with 42 age-, sex-, and tumor stage-matched dogs that received piroxicam alone. Compared to the dogs treated with piroxicam alone, those administered the lapatinib/piroxicam treatment had a greater reduction in the size of the primary tumor and improved survival. Exploratory analyses showed that HER2 overexpression was associated with response and survival in dogs treated with lapatinib. Our study suggests that lapatinib showed encouraging durable response rates, survival, and tolerability, supporting its therapeutic use for untreated advanced urothelial carcinoma in dogs. The use of lapatinib as a first-line treatment may be investigated further in human patients with urothelial carcinoma.
Longer than 2 hours to antibiotics is associated with doubling of mortality in a multinational community-acquired bacterial meningitis cohort
Authors: Eisen Damon P. , Hamilton Elizabeth , Bodilsen Jacob , Køster-Rasmussen Rasmus , Stockdale Alexander J. , Miner James , Nielsen Henrik , Dzupova Olga , Sethi Varun , Copson Rachel K. , Harings Miriam , Adegboye Oyelola A. 
Published: January 2022

AbstractTo optimally define the association between time to effective antibiotic therapy and clinical outcomes in adult community-acquired bacterial meningitis. A systematic review of the literature describing the association between time to antibiotics and death or neurological impairment due to adult community-acquired bacterial meningitis was performed. A retrospective cohort, multivariable and propensity-score based analyses were performed using individual patient clinical data from Australian, Danish and United Kingdom studies. Heterogeneity of published observational study designs precluded meta-analysis of aggregate data (I2 = 90.1%, 95% CI 71.9–98.3%). Individual patient data on 659 subjects were made available for analysis. Multivariable analysis was performed on 180–362 propensity-score matched data. The risk of death (adjusted odds ratio, aOR) associated with treatment after two hours was 2.29 (95% CI 1.28–4.09) and increased substantially thereafter. Similarly, time to antibiotics of greater than three hours was associated with an increase in the occurrence of neurological impairment (aOR 1.79, 95% CI 1.03–3.14). Among patients with community-acquired bacterial meningitis, odds of mortality increase markedly when antibiotics are given later than two hours after presentation to the hospital.
Physical exertion at work and addictive behaviors: tobacco, cannabis, alcohol, sugar and fat consumption: longitudinal analyses in the CONSTANCES cohort
Authors: Hamieh Nadine , Descatha Alexis , Zins Marie , Goldberg Marcel , Czernichow Sébastien , Hoertel Nicolas , Plessz Marie , Roquelaure Yves , Limosin Frédéric , Lemogne Cédric , Matta Joane , Airagnes Guillaume 
Published: January 2022

AbstractWe examined the prospective association of physical exertion at work with subsequent tobacco, cannabis, alcohol use, and sugar and fat consumption. Volunteers of the French population-based CONSTANCES cohort currently employed were included from 2012 to 2017 for tobacco and cannabis outcomes (n = 100,612), and from 2012 to 2016 for alcohol and sugar and fat outcomes (n = 75,414). High level of physical exertion at work was defined as a score ≥ 12 at the Rating Perceived Exertion Borg scale. Substance use was self-reported and diet rich in sugar and fat was obtained from principal component analysis and analyzed as quartiles. Generalized linear models computed odds of substance use and sugar and fat consumption at follow-up according to baseline physical exertion at work, while adjusting for sociodemographic factors, depressive symptoms and baseline level of consumption. High physical exertion was associated with tobacco use with dose-dependent relationships. It was also associated with increased odds of cannabis use at least once per month compared to no use in the past and with increased odds of diet rich in sugar and fat. Hence, the role of physical exertion at work on tobacco and cannabis use and diet rich in sugar and fat should be tackled for information and prevention strategies.
Comparison of parallel infill sampling criteria based on Kriging surrogate model
Authors: Chen Cong , Liu Jiaxin , Xu Pingfei 
Published: January 2022

AbstractOne of the key issues that affect the optimization effect of the efficient global optimization (EGO) algorithm is to determine the infill sampling criterion. Therefore, this paper compares the common efficient parallel infill sampling criterion. In addition, the pseudo-expected improvement (EI) criterion is introduced to minimizing the predicted (MP) criterion and the probability of improvement (PI) criterion, which helps to improve the problem of MP criterion that is easy to fall into local optimum. An adaptive distance function is proposed, which is used to avoid the concentration problem of update points and also improves the global search ability of the infill sampling criterion. Seven test problems were used to evaluate these criteria to verify the effectiveness of these methods. The results show that the pseudo method is also applicable to PI and MP criteria. The DMP and PEI criteria are the most efficient and robust. The actual engineering optimization problems can more directly show the effects of these methods. So these criteria are applied to the inverse design of RAE2822 airfoil. The results show the criterion including the MP has higher optimization efficiency.
Optimizing the geodetic networks based on the distances between the net points and the project border
Authors: AbdAllah Ahmed A. G. , Wang Zhengtao 
Published: January 2022

AbstractGeodetic networks are important for most engineering projects. Generally, a geodetic network is designed according to precision, reliability, and cost criteria. This paper provides a new criterion considering the distances between the Net Points (NPs) and the Project Border (PB) in terms of Neighboring (N). Optimization based on the N criterion seeks to relocate the NPs as close as possible to PB, which leads to creating shorter distances between NPs or those distances linking NPs with Target Points (TPs) to be measured inside PB. These short distances can improve the precision of NPs and increase the accuracy of observations and transportation costs between NPs themselves or between NPs and TPs (in real applications). Three normalized N objective functions based on L1, L2, and L∞‒norms were formulated to build the corresponding N optimization models, NL1; NL2; and NL∞ and to determine the best solution. Each model is subjected to safety, precision, reliability, and cost constraints. The feasibility of the N criterion is demonstrated by a simulated example. The results showed the ability of NL∞ to determine the safest positions for the NPs near PB. These new positions led to improving the precision of the network and preserving the initial reliability and observations cost, due to contradiction problems. Also, N results created by all N models demonstrate their theoretical feasibility in improving the accuracy of the observations and transportation cost between points. It is recommended to use multi-objective optimization models to overcome the contradiction problem and consider the real application to generalize the benefits of N models in designing the networks.
The interaction of high-power fiber laser irradiation with intrusive rocks
Authors: Seo Youngjin , Lee Dongkyoung , Pyo Sukhoon 
Published: January 2022

AbstractLaser cutting of intrusive rocks, including granite, gabbro, and diorite, is carried out in order to assess the cut characteristics through geometrical measurements, such as kerf width, melting width, and penetration depth. The absorption rate for each specimen at the wavelength of 1064 nm is measured using a spectrophotometer. A multimode fiber laser is used in this study with the power of 9 kW and different cutting speeds. Furthermore, nitrogen gas at 13 bar is applied as the assistant gas in order to remove the melted material effectively. As a result of the experiment, the relationship between the cutting speed and geometrical measurements is investigated. Furthermore, variations of penetration depth are performed in accordance with the number of laser cuts. In addition, through energy dispersive X-ray (EDX) element mapping, minerals that comprise the rocks are classified and characterized. Subsequently, the changes in the microstructure and chemical composition of each specimen, before and after laser cutting, are compared using scanning electron microscope (SEM) and EDX analyses. Experimental results demonstrate that the cutting characteristics vary, depending on the types of minerals that make up the rock. Based on a series of tests, it is identified that volume energy of more than 3.06E + 13 $$\mathrm{J}/{\mathrm{m}}^{3}$$

J
/

m

3

is required to fully cut intrusive rocks that have a thickness of 25 mm.
The field monitoring experiment of the high-level key stratum movement in coal mining based on collaborative DOFS and MPBX
Authors: Wang Xiaozhen , Xie Jianlin , Zhu Weibing , Xu Jialin 
Published: January 2022

AbstractThe deformation and movement characteristics of high-level key stratums in overlying strata are important for estimating ground subsidence and understanding failure characteristics of ultrathick strata during mining. In this study, a distributed optical fiber sensor (DOFS) and multipoint borehole extensometers (MPBXs) were collaboratively employed to monitor the deformation of high-level key stratums in situ during the mining process at working face 130,604 of the Maiduoshan Coal Mine. DOFS monitoring results showed that the distance from advance influence of mining on the ground surface is 219.2 m. The deformation of the shallow stratums were greater and was affected earlier than that of the deep stratums. The deformation in the strata did not occur continuously and the boundary curve of the impact from advance mining was not a straight line with the advancement of the working face. By the MPBX technology, we measured the strata movement and obtained four-stage characteristics of high-level key stratum movement. The subsidence of the primary key stratum and the sub key stratum were monitored to reach 1389 and 1437 mm; their final relative displacement differed by 48 mm. No bed separation was observed in between the strata, and the key stratums tended to sink as a whole with the advancement of the working face. This research guides the analysis the movement of thick high-level key stratums.
Comparison of oral metabolome profiles of stimulated saliva, unstimulated saliva, and mouth-rinsed water
Authors: Maruyama Yuki , Nishimoto Yuichiro , Umezawa Kouta , Kawamata Ryosuke , Ichiba Yuko , Tsutsumi Kota , Kimura Mitsuo , Murakami Shinnosuke , Kakizawa Yasushi , Kumagai Takashi , Yamada Takuji , Fukuda Shinji 
Published: January 2022

AbstractSaliva includes a substantial amount of biological information, which has enabled us to understand the relationship between oral metabolites and various oral and systemic disorders. However, collecting saliva using a controlled protocol is time-consuming, making saliva an unsuitable analyte in large cohort studies. Mouth-rinsed water (MW), the water used to rinse the mouth, can be collected easily in less time with less difference between subjects than saliva and could be used as an alternative in oral metabolome analyses. In this study, we investigated the potential of MW collection as an efficient alternative to saliva sample collection for oral metabolome profiling. MW, stimulated saliva, and unstimulated saliva were collected from 10 systemically healthy participants. The samples were subjected to metabolome analysis using capillary electrophoresis time-of-flight mass spectrometry, and the types and amounts of metabolites in the samples were compared. Qualitatively, MW contained the same metabolites as unstimulated and stimulated saliva. While the quantity of the metabolites did not drastically change between the sampling methods, all three reflected individual differences, and the features of MW were the same as those of the unstimulated saliva. Overall, these results suggest that MW may be an appropriate alternative to saliva in oral metabolome profile analysis.
Expression of glutamate carboxypeptidase II in the glial tumor recurrence evaluated in vivo using radionuclide imaging
Authors: Kunikowska Jolanta , Czepczyński Rafał , Pawlak Dariusz , Koziara Henryk , Pełka Kacper , Królicki Leszek 
Published: January 2022

AbstractGlutamate carboxypeptidase II (GCP), also known as prostate specific membrane antigen (PSMA) has been found to be expressed in glioma vasculature in in-vitro studies. GCP expression can be traced with the use of [68Ga]Ga-PSMA-11 PET/CT used routinely for prostate cancer imaging. The aim of this paper was to analyze GCP expression in the recurrent glial tumors in vivo. 34 patients (pts.) aged 44.5 ± 10.3 years with suspicion of recurrence of histologically confirmed glioma grade III (6 pts.) and grade IV (28 pts.) were included in the study. All patients underwent contrast-enhanced MR and [68Ga]Ga-PSMA-11 PET/CT. No radiopharmaceutical-related adverse events were noted. PET/CT was positive in all the areas suspected for recurrence at MR in all the patients. The recurrence was confirmed by histopathological examinations or follow-up imaging in all cases. The images showed a very low background activity of the normal brain. Median maximal standard uptake value (SUVmax) of the tumors was 6.5 (range 0.9–15.6) and mean standard uptake value (SUVmean) was 3.5 (range 0.9–7.5). Target-to-background (TBR) ratios varied between 15 and 1400 with a median of 152. Target-to-liver background ratios (TLR) ranged from 0.2 to 2.6, the median TLR was 1.3. No significant difference of the measured parameters was found between the subgroups according to the glioma grade. High GCP expression in the recurrent glioma was demonstrated in-vivo with the use of [68Ga]Ga-PSMA-11 PET/CT. As the treatment options in recurrent glioma are limited, this observation may open new therapeutic perspectives with the use of radiolabeled agents targeting the GCP.
Controllable atom-photon entanglement via quantum interference near plasmonic nanostructure
Authors: Sangshekan Behzad , Sahrai Mostafa , Asadpour Seyyed Hossein , Poursamad Bonab Jafar 
Published: January 2022

AbstractA five-level atomic system is proposed in vicinity of a two-dimensional (2D) plasmonic nanostructure with application in atom-photon entanglement. The behavior of the atom-photon entanglement is discussed with and without a control laser field. The amount of atom-photon entanglement is controlled by the quantum interference created by the plasmonic nanostructure. Thus, the degree of atom-photon entanglement is affected by the atomic distance from the plasmonic nanostructure. In the presence of a control field, maximum entanglement between the atom and its spontaneous emission field is observed.
Zika virus baculovirus-expressed envelope protein elicited humoral and cellular immunity in immunocompetent mice
Authors: Shin Minna , Kim Kiju , Lee Hyo-Ji , Lee Rangyeon , Jung Yu-Jin , Park Jeongho , Hahn Tae-Wook 
Published: January 2022

AbstractZika virus (ZIKV) is a mosquito-borne virus that has a high risk of inducing Guillain–Barré syndrome and microcephaly in newborns. Because vaccination is considered the most effective strategy against ZIKV infection, we designed a recombinant vaccine utilizing the baculovirus expression system with two strains of ZIKV envelope protein (MR766, Env_M; ZBRX6, Env_Z). Animals inoculated with Env_M and Env_Z produced ZIKV-specific antibodies and secreted effector cytokines such as interferon-γ, tumor necrosis factor-α, and interleukin-12. Moreover, the progeny of immunized females had detectable maternal antibodies that protected them against two ZIKV strains (MR766 and PRVABC59) and a Dengue virus strain. We propose that the baculovirus expression system ZIKV envelope protein recombinant provides a safe and effective vaccine strategy.