bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transparent liver tumor imaging for transarterial chemoembolization (TACE): cancer treatment mechanism in TACE
Authors: Hong Sera , Choi Won Seok , Purushothaman Baskaran , Koh Jaemoon , Kim Hyo-Cheol , Chung Jin Wook , Song Joon Myong , Choi Jin Woo 
Published: June 2022

Abstract
Background: Hepatocellular carcinoma (HCC) is the most common primary liver cancer and a leading cause of cancer-related deaths worldwide. Transarterial chemoembolization (TACE) is the current standard of care for patients with intermediate-stage HCC. While TACE has been widely used for the treatment of HCC, there are unsolved questions on the use of chemotherapeutics agents in TACE. TACE has been thought to induce necrosis in HCC due to the complex effects of embolism and intra-arterial delivery of anticancer drugs; however, it is unclear how far a drug is delivered from the arteries before the tumor undergoes necrosis induced by embolization-induced ischemia.Methods: In this study, the drug delivery effect, time-dependent embolic effect, and immunogenic response were compared following conventional TACE (cTACE), drug-eluting embolic TACE (DEE-TACE), and transarterial embolization (TAE) in a rat model of HCC. Following each treatment, three-dimensional drug delivery was evaluated via the transparent liver tumor imaging. Time-dependent tumor necrosis was analyzed by serial tumor harvesting and histological staining. Early (24-h) and residual (7-day) immune responses were evaluated by flow cytometric analyses and immunohistochemistry. Results: The intratumoral blood vessel structure became damaged after treatment due to embolization-induced ischemia. The more severe the vascular damage caused over time or by treatment type, the greater the distance traveled by drugs. In the first 24-h, most chemotherapeutic agents were located within 80 and 110 µm of the nearest vessels after cTACE and DEE-TACE, respectively, while 85.3–97.2% of tumor cells underwent necrosis. The percentages of CD4 and IL-17+ CD4 T cells were significantly increased following treatment, but drug loading did not affect the early and residual immune responses. Conclusion: Transarterially delivered chemotherapeutic agents appeared to exert a limited role, owing to the rapid and overwhelming effect of embolization.
A case report of ectopic adrenocorticotropic hormone syndrome in a patient with hepatocellular carcinoma
Authors: Amoateng Magdelene , Ibrahim Eiman Y , Rodriguez Abraham , Vera Armando , Hulinksy Ilja 
Published: June 2022

Abstract
Background:Accurate and early diagnosis of Cushing’s syndrome is challenging. Ectopic production of adrenocorticotropic hormone (ACTH) by carcinoid tumors is relatively rare and may not be detected.The tumors that produce ectopic ACTH are usually bronchopulmonary or thymic in origin and are rarely of gastrointestinal origin. To our knowledge, there is only one published case of an ectopic ACTH secreting primary hepatic carcinoid tumor with consistent biochemical markers and symptoms that resolved with resection.Patient findings:This report describes a rare case of a patient who had Cushing syndrome from the ectopic secretion of adrenocorticotropic hormone by a hepatocellular carcinoma (HCC) with neuroendocrine features.Conclusions:Ectopic ACTH secretion is typically a clinical diagnosis that is confirmed after surgical resection of the ectopic source. Only few reports were found to describe ectopic ACTH secretion in HCC. Our case illuminates the significance of clinical and immunohistochemical studies to establish the appropriate diagnosis in patients with tumors believed to secrete ACTH. Additionally, absent immunostaining has been also described in some patients with ectopic ACTH secretion.
Clinical value of serum lncRNA MIAT in lung cancer
Authors: hou yulei , Zhang Jianhong , He Jing , Zhang Xiaoyue , Yuan Jinshan , Miao Guihua , Tang Wei , Chen Hui 
Published: June 2022

Abstract
The long noncoding RNA myocardial infarction associated transcript (MIAT) played a critical role in lung cancer. Present study aimed to measure serum MIAT levels and investigate its clinical value in lung cancer. Serum was collected from 109 lung cancer and 61 healthy controls, and MIAT levels were measured using RT-qPCR and compared. The relations between serum MIAT and the clinical characteristics of cancer were explored. Diagnostic power of MIAT for lung cancer was analyzed by plotting an ROC curve. Serum MIAT levels were significantly higher in lung cancer than in healthy controls (t = 3.288, P < 0.01). Serum MIAT levels were increased significantly in patients at middle-late stages than in those at early stages(t = 2.862, P < 0.01), and significantly higher in patients with lymph node metastasis than in those without (t = 4.112, P < 0.01). The cut-off value of serum MIAT for the diagnosis of lung cancer was 0.945, with a sensitivity of 66.1%, specificity of 63.9%, and area under the curve of 0.65 (95% confidence interval, 0.57–0.73). Serum MIAT levels were increased significantly among lung cancer and related to TNM stage or lymph node metastasis, suggesting the potential of MIAT as a marker of lung cancer.
Factors influencing consistent use of bed nets for the control of malaria among children under five years in Soroti District, North Eastern Uganda
Authors: Akello Anne Ruth , Byagamy John Paul , Etajak Samuel , Okadhi Charles Stephen , Yeka Adoke 
Published: June 2022

Abstract
Background: The use of insecticide treated bed nets has been proven to be effective in reducing malaria transmission in highly endemic areas. Use of Long-Lasting Insecticide-treated Nets (LLINs) has been embraced by many malaria endemic countries. The LLINs are up to 95% effective in inhibiting blood feeding when used consistently even after seven years. The challenge however is enhancing their consistent use, especially by the most vulnerable groups (children under five years and pregnant women). The study established factors associated with consistent use of bed nets for malaria control among children under five years in Soroti district.Methods: The study employed a cross-sectional design, with multi-stage sampling of households. A total of 400 Households (HH) were sampled and the HH head in each household interviewed. Key Informant Interviews (KIIs) were conducted with 7 key informants who were knowledgeable on the subject matter. Data analysis was done using SPSS 17.0 at Univariate, Bivariate and Multivariable levels; after entry and cleaning. Key informants’ data were summarized manually; verbatim quotes and text used to reinforce quantitative data in line with objectives.Results: Only 56.8% of the 690 children under five years used bed nets consistently. The factors affecting consistent bed net use were age of the child, their use of bed nets the previous night, occupation of caretaker, respondents’ perceived susceptibilty, perceived risk of getting malaria, size and shape of the bed nets. Rectangular nets were difficult to hang daily in huts according to most key informants. Conclusion: Consistent bed net use among under fives is still below the RBM target of 85% by 2015 and can be enhanced by providing conical bed nets and setting aside a health education program to emphasize the effectiveness of even one mosquito in spreading malaria at night to the entire household and ability of bed nets to stop transmission better than other methods.
A Novel Method for Solving Response of Stochastic Vibro-impact Systems with Two-sided Barriers
Authors: Ma Shichao , Ning Xin , Wang Liang , Jia Wantao , Han Ping 
Published: June 2022

Abstract
The research of stochastic steady-state response of vibration systems including bilateral impacts and gaps has important theoretical and practical significance. At present, conventional analytical methods destroy the inherent non-smooth of the systems because of many approximate transformations when solving response. In order to deal with this problem, we propose a new method to preserve the velocity jump under the bilateral impact without nonsmooth transformation in this paper. A complete process is regarded as the one-step transition probability that the stochastic trajectories start from one barrier, and return to the original barrier after impacting with another barrier. Then, the intervals of response are established based on the initial barrier to ensure of the continuity of the state space. We analyzed the steady-state response of a bilateral Rayleigh vibro-impact oscillator and a piezoelectric energy harvesting device with two symmetric barriers by utilizing the proposed method, respectively. Comparing with Monte Carlo simulations, it is fully demonstrated the effectiveness of this method. At the same time, it is found that the cases using our proposed method have wide applicability under different position of barriers, restitution coefficient, noise excitation and system parameters.
Projection and Assessment of Performance of Software Reuse Using Data Mining
Authors: Jha Pooja 
Published: June 2022

Abstract
In today’s world, there is improvement in hardware performance, storage capacity, architectures. So, there exists an opportunity to design and control the software based on requirements. Software reuse is an optimistic approach to achieve software productivity and software quality. The paper presents a method for predicting and analysing performance of software reuse to understand whether reuse can lead to success or failure of the product. The technique of data mining is exploited to improve the Accuracy, Error rate, and Kappa statistics. It is supported by the Weka-tool. The pre-processing filtering techniques are used to improve the speed of computation by removing the missing values. To validate the output of proposed algorithm comparison is done with J48 classifier.
Unravelling interplay between molecule-based spin-state switching and electron transport in a single-crystal 3D metal-organic framework
Authors: Costa José , Martinez-Martinez Ana , Piñeiro-Lopez Lucia , Resines-Urien Esther , Fernandez-Blanco Angel , Mariano Antonio , Poloni Roberta , Rodriguez-Velamazan Jose Alberto , Sañudo Eva , Burzurí Enrique 
Published: June 2022

Abstract
Molecule-based Spin Crossover (SCO) materials display likely one of the most spectacular switchable processes. The SCO involves reversible changes in their physicochemical properties (i.e. optical, magnetic, electronic and elastic) that are coupled with the spin-state change under an external perturbation (i.e. temperature, light, magnetic field or the inclusion of analytes). Although very promising for their future integration into electronic devices, most of the SCO compounds show two major drawbacks: i) their intrinsic low conductance and ii) the unclear mechanism connecting the spin-state change and the electrical conductivity. Herein, we report the controlled single-crystal-to-single-crystal temperature-induced transformation in a robust metal-organic framework (MOF), [Fe2(H0,67bdt)3]·9H2O (1), being bdt2− = 1,4-benzeneditetrazolate, exhibiting a dynamic spin-state change concomitant with an increment in the anisotropic electrical conductance. 1 remains intact during the SCO process even after approximately a 15% volume reduction. The experimental and theoretical rationalization of the electronic orbital delocalization in the MOF points to a direct correlation between the spin-state of the iron and the electronic conductivity of the 3D structure.
Observer-based event-triggered optimal control for unknown nonlinear stochastic multi-agent systems with input constraints
Authors: Liu Chen , Liu Lei , Wu Zhaojing , Cao Jinde , Qiu Jianlong 
Published: June 2022

Abstract
This paper addresses the observer-based event-triggered optimal control (ETOC) for nonlinear Itô-type stochastic multi-agent systems (SMASs) with unknown internal states and input constraints. To begin with, the event-triggered stochastic Hamilton-Jacobi-Bellman (HJB) equation with input constraints is presented for the first time. Next, the observer-based identifier network is utilized to recover the knowledge of unknown system dynamics. After that, the approximate event-triggered optimal controller is designed via adaptive critic designs (ACDs) whose weights are only updated at the triggering instants. It is worth mentioning that there is no published literature on the ETOC for nonlinear SMASs with unknown internal states and input constraints via the framework of ET-ACDs. This study is the first attempt to solve this problem. Moreover, it is also proved that the Zeno behavior does not exist in the closed-loop system. Finally, we present two examples to further verify the validity of the ETOC scheme.
Airway and lung organoids from human induced pluripotent stem cells can be used to assess CFTR conductance
Authors: Demchenko Anna , Kondrateva Ekaterina , Tabakov Vyacheslav , Efremova Anna , Salikhova Diana , Bukharova Tatiana , Goldshtein Dmitry , Balyasin Maxim , Bulatenko Natalia , Amelina Elena , Lavrov Alexander , Smirnikhina Svetlana 
Published: June 2022

Abstract
Background: Airway (AOs) and lung (LOs) organoids derived from human induced pluripotent stem cells (hiPSCs) are current models for personalized drug screening, cell-cell interaction studies, and lung disease research. We analyzed the existing differentiation protocols and identified the optimal conditions for obtaining organoids. In this article, we describe a step-by-step protocol for differentiating hiPSCs into airway and lung organoids.Methods: hiPSCs were differentiated into AOs and LOs according to previously published protocols with modification of some stages of differentiation. Immunocytochemical staining, flow cytometry, and confocal microscopy were performed to determine the cellular composition of AOs and LOs. To assess the functional activity of the CFTR channel, forskolin-induced swelling (FIS) in AOs and LOs was performed. FIS images were analysed with ilastik and CellProfiler software. Statistical analysis of data was performed using GLMM models with post-hoc Tukey's tests.Results: Airway and lung organoids obtained from a healthy donor and from two donors with a homozygous F508del mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene and demonstrated that the airway organoid area was significantly different. Analysis of the cellular composition of airway and lung organoids showed that airway organoids contain proximal lung epithelial cells, while lung organoids contain both proximal and distal lung epithelial cells. Forskolin-induced swelling of organoids derived from a healthy donor showed that lung organoids, as well as airway organoids, contain functional epithelial cells and swell after 24-hour exposure to forskolin, which makes it a good model for analyzing the CFTR channel conductance in vitro. Conclusions: This study demonstrate the feasibility of generating and characterizing airway and lung organoids from hiPSCs, which can be used for a variety of future applications, such as assess the CFTR channel conductance, drug screening, disease modeling and other.
MELTF might regulate ferroptosis, pyroptosis, and autophagy in Platelet-Rich Plasma-mediated endometrial epithelium regeneration
Authors: Mao Yanhong , Wang Mei , Xiong Yao , Wen Xue , Zhang Ming , Ma Ling , Zhang Yuanzhen 
Published: June 2022

Abstract
Endometrial basal layer is essential for the endometrial regeneration, whose disruption leads to thin endometrium or intrauterine adhesion (IUA) with unsatisfactory prognosis. Emerging data indicate that platelet-rich plasma (PRP) can promote endometrial proliferation, but the mechanism by which PRP regulates endometrial regeneration remains unclear. Herein, we investigated the therapeutic effects and possible mechanisms of PRP on endometrial regeneration. IUA animal model was generated by sham, mechanically damaging endometrium with or without PRP for 10 days. Uterine section in Model group showed degenerative changes with narrow endometrial lumen, atrophic columnar epithelium, decreased number of endometrial glands, decreased endometrial thickness and increased collagen deposition. The above disruption could be ameliorated by the PRP. Transcriptome sequencing analysis displayed that retinol metabolism pathway and extra cellular matrix (ECM) receptor interaction pathway were up-regulated and enriched in differential expression gene (DEGs). Melanotransferrin (MELTF) was the key up-regulated gene in PRP-induced endometrial regeneration, which was verified in vivo and in vitro. Ferroptosis, autophagy, and pyroptosis were down-regulated in PRP-treated Ishikawa cells. Conclusively, PRP might promote the endometrium regeneration through up-regulating the retinol metabolism and ECM receptor interaction pathway with MELTF. Meanwhile, PRP could also inhibit endometrial epithelial cells death by regulating ferroptosis, autophagy, and pyroptosis.
Effects of Open-Source Image Preprocessing on Glaucoma and Glaucoma Suspect Fundus Image Differentiation with CNN
Authors: Seker Mustafa Ege , Koyluoglu Yilmaz Onat , Celebi Ali Riza Cenk , Bayram Bugrahan 
Published: June 2022

Abstract
Purpose: Investigating whether a Keras-based Convolutional Neural Networks (CNN) model could detect glaucoma suspect cases from glaucoma cases without a visual field test and the effects of open-source data preprocessing in AI based glaucoma detection.Methods: 398 glaucoma and 378 glaucoma suspect cases approved by a glaucoma specialist ophthalmologist were enrolled in this study. Fundus images were retrieved from an optical coherence tomography device. An open-source graphic software was used to create training sets. There were three distinct groups: fundus-centered cropped images, grayscale version of said images with an auto white balance option to enhance the features and in addition to the conversions stated above, horizontal, vertical, and horizontal plus vertical flips were applied in the third group. Cropped images were used to train our Keras-based CNN model with 49 deep layers. Model fit was designed at 50 epochs per run and the performance metrics for each run were recorded. Normality was assessed with the Shapiro-Wilk test. A one-way ANOVA was applied to analyze each image set's validation accuracy. Bonferroni corrections were applied if appropriate. Demographics of the patients were analyzed with the Mann-Whitney-U test and the chi-2 test.Results: The mean age of glaucoma patients and glaucoma suspected patients showed a statistically significant difference (P-value < 0.001, mean ± standard deviation 62 ± 15 and 45 ± 15 respectively). Gender did not show a statistically significant difference between groups (P-value = 0.388). Validation accuracy scores in groups 1,2 and 3 were 0.71 ± 0.02, 0.77± 0.02 and 0.85±0.03 respectively (mean ± standard deviation) (P-value < 0.001). The sensitivities and specificities between groups were different and those differences were found to be statistically significant (P-value < 0.001 and P-value < 0.001, respectively).Conclusion: In this report, we introduce open-source and easy-to-deploy image pre-processing methods to improve the outcome of glaucoma detection from glaucoma suspected cases in stereoscopic optic disc photography-derived fundus images that could be used with any CNN-based computer-aided diagnosis system without requiring a visual field test, contributing to decreasing the burden associated with undiagnosed glaucoma progression.
Oncological and Functional Outcomes of High-Risk and Very High-Risk Prostate Cancer Patients after Robot-Assisted Radical Prostatectomy
Authors: Chen Wei-Hsin , Lee Yu Khun , Kuo Hann-Chorng , Wang Jen-Hung , Jiang Yuan-Hong 
Published: June 2022

Abstract
This study investigated the oncological and functional outcomes of robot-assisted radical prostatectomy (RaRP) in high-risk and very high-risk prostate cancer patients. One hundred prostate cancer patients who underwent RaRP were retrospectively enrolled, including 53 in the below high-risk group and 47 in the high-risk/very high-risk group. The mean age was 69.7 ± 7.4 years with a median follow-up of 26.4 (range 3.3–71.3) months. The median biochemical recurrence (BCR)-free survival of the entire cohort was 53.1 months. The high-risk/very high-risk group without adjuvant treatment had significantly worse BCR-free survival than the high-risk/very high-risk group with adjuvant treatment and the below high-risk group (19.6 vs. 60.5 and 53.1 months, p = 0.0015). The rates of postoperative stress urinary incontinence (SUI) at 1 week, 1 month, and 12 months were 50.7%, 43.7%, and 8.5%, respectively. High-risk/very high-risk patients had significantly higher rates of SUI at postoperative week 1 (75.8% vs. 28.9%) and month 1 (63.6% vs. 26.3%) than the below high-risk group (both p < 0.01). High-risk and very high-risk patients who received both RaRP and adjuvant treatment had BCR-free survival rates comparable with the below high-risk patients. High or very high-risk impeded early but not long-term recovery of continence after RaRP.
Association between sensitivity to thyroid hormone indices and the risk of osteoarthritis: an NHANES study
Authors: Chen Shuai , Sun Xiaohe , Zhou Guowei , Jin Jie , Li Zhiwei 
Published: June 2022

Abstract
Objectives:Thyroid hormones play an instrumental role in chondrogenic differentiation and matrix maturation. However, studies investigating the relationship between thyroid function and the risk of osteoarthritis (OA) remain scarce. This study was designed to investigate the correlation between thyroid status and OA from a novel perspective of sensitivity to thyroid hormones.Methods:The study included 8,478 people from the National Health and Nutrition Examination Survey (NHANES) 2007-2010. The sensitivity to thyroid hormone indices included Thyrotroph Thyroxine Resistance Index (TT4RI), Thyroid-stimulating hormone (TSHI), Thyroid Feedback Quantile-based Index (TFQI), and Free Triiodothyronine /Free thyroxine (FT3/FT4), which were calculated based on serum free triiodothyronine (FT3), free thyroxine (FT4), and thyroid stimulating hormone (TSH). Considering the complex survey design and sample weights, we employed multivariate linear regression models and stratified analysis to evaluate the correlation between sensitivity to thyroid hormone indices and OA. Results:Study results indicated that participants with OA had elevated TT4RI, TSHI, and TFQI levels, and lower FT3/FT4 levels compared to those with non-arthritis. After adjusting for other covariates, FT3/FT4 was negatively associated with the risk of OA (OR= 1.162, 95%CI: 1.048-1.478, P= 0.021); (OR= 1.261, 95%CI: 1.078-1.623, P= 0.042). In subgroup analyses stratified by gender and BMI, participants with OA had higher TFQI levels compared to those without OA in both genders. (OR= 1.491, 95%CI: 1.070-2.077, P= 0.018); (OR= 2.548, 95%CI: 1.929-3.365, P<0.001). The higher TFQI levels were consistently associated with the increased prevalence of OA in the BMI (<18.5 kg/m2) group after adjusting for different covariates, but not in other BMI groups. In, addition, TFQI performed better than FT3/FT4, TSHI, and TT4RI on ROC analyses for OA prediction. Conclusions:The levels of FT3/FT4, TSHI, TT4RI, and TFQI are strongly associated with the prevalence of OA, which illustrates the complex correlation between the thyroid system and chondrogenic differentiation. TFQI may be used as a helpful indicator to predict OA and provide novel ideas for the evaluation and treatment of OA.