bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sarcopenia, healthy living, and mortality in patients with chronic liver diseases
Authors: Van Dongen Catherine , Paik James M. , Harring Michael , Younossi Youssef , Price Jillian K. , Kabbara Khaled , Golabi Pegah , Younossi Zobair M. 
Published: August 2022

Abstract
Chronic liver diseases (CLDs) are associated with increased morbidity and mortality. Sarcopenia is an important complication of CLD that can be impacted by several modifiable risk factors. Our aim was to assess the associations between healthy living, sarcopenia, and long‐term outcomes among patients with CLD. We used the Third National Health and Nutrition Examination Survey data with National Death Index–linked mortality files. We used the American Heart Association's Life's Simple 7 (LS7) metrics as surrogates of healthy living. The study included 12,032 subjects (34.9% CLDs [0.5% hepatitis B virus (HBV), 1.8% hepatitis C virus (HCV), 5.7% alcohol‐associated liver disease (ALD), 26.9% nonalcoholic fatty liver disease (NAFLD)] and 65.1% controls). Prevalence of sarcopenia was higher among NAFLD than other CLDs and the controls (40.7% in NAFLD, 27.2% in ALD, 22.4% in HCV, 16.8% in HBV, and 18.5% in controls; p < 0.001). Among NAFLD and ALD, patients with sarcopenia were less likely to meet ideal LS7 metrics than those without sarcopenia. During 27 years of follow‐up, among 4 patients with CLDs and the controls, all‐cause cumulative mortality was highest among patients with HCV (35.2%), followed by ALD (34.7%) and NAFLD (29.6%). The presence of sarcopenia was associated with higher risk of all‐cause mortality only among subjects with NAFLD (hazard ratio [HR] 1.24; 95% confidence interval [CI] 1.01–1.54; p = 0.04). Among subjects with NAFLD, presence of sarcopenia was associated with higher risk of cardiovascular‐specific (HR 2.28 [1.71–3.05; p < 0.01]), cancer‐specific (HR 1.90 [1.37–2.65]; p < 0.01), diabetes‐specific (HR 6.42 [2.87–14.36]; p < 0.01), and liver‐specific mortality (HR 2.49 [1.08–5.76]; p = 0.04). The multivariable model showed that component of LS7 metrics that provided the strongest protection against sarcopenia were ideal body mass index, ideal blood pressure, ideal physical activity, and ideal glycemic control among subjects with NAFLD subjects. Conclusions: Among subjects with NAFLD, sarcopenia is associated with a higher risk of all‐cause mortality and liver mortality. Attainment of ideal LS7 metrics provides protection against sarcopenia in NAFLD.
Assessment of attrition and retention factors in the oncology pharmacy workforce: Results of the oncology pharmacy workforce survey
Authors: Rao Kamakshi V. , Gulbis Alison M. , Mahmoudjafari Zahra 
Published: August 2022

AbstractIntroductionHospital departments of pharmacy are experiencing significant challenges in the recruitment and retention of trained hematology‐oncology pharmacists. The reasons for these challenges are varied, but one consistent challenge is the growing attrition of clinically‐trained pharmacists from the patient care environment.MethodsAn electronic 21‐item survey was distributed to oncology pharmacists across the United States. The survey assessed work environment, board certification, clinical commitment, and other factors. How those factors might be associated with reported job satisfaction and risk for attrition was examined.ResultsA total of 607 individuals responded to at least one of the survey questions, with the majority representing those actively in clinical practice. The results demonstrate that the oncology pharmacy workforce is a highly trained and capable one, with a high level of satisfaction with their work. That said, over 60% of those who responded indicated that they were either actively seeking or open to alternate employment outside of the patient care environment. The largest contributing factors to attrition risk include untenable work burdens, burnout, lack of work‐life integration, and ineffective leadership, while commitments to clinical work were associated with improved job satisfaction and decreased attrition risk.ConclusionThese results bring to light the current state of satisfaction and attrition risk for the oncology pharmacy workforce nationwide. A significant opportunity exists to improve the experience of these pharmacists, particularly in the patient care environment. Based on these findings, departmental, organizational, and national leadership must pursue more tenable work burdens, better value and recognition measures, and more accurate and meaningful metrics for clinical pharmacists in order to retain this important workforce.
Effects of cell seeding technique and cell density on BMP‐2 production in transduced human mesenchymal stem cells
Authors: Collon Kevin , Bell Jennifer A. , Chang Stephanie W. , Gallo Matthew C. , Sugiyama Osamu , Marks Carolyn , Lieberman Jay R. 
Published: August 2022

AbstractSmall animal models have demonstrated the efficacy of ex vivo regional gene therapy using scaffolds loaded with BMP‐2‐expressing mesenchymal stem cells (MSCs). Prior to clinical translation, optimization of seeding techniques of the transduced cells will be important to minimize time and resource expenditure, while maximizing cell delivery and BMP‐2 production. No prior studies have investigated cell‐seeding techniques in the setting of transduced cells for gene therapy applications. Using BMP‐2‐expressing transduced adipose‐derived MSCs and a porous ceramic scaffold, this study compared previously described static and dynamic seeding techniques with respect to cell seeding efficiency, uniformity of cell distribution, and in vitro BMP‐2 production. Static and negative pressure seeding techniques demonstrated the highest seeding efficiency, while orbital shaking was associated with the greatest increases in BMP‐2 production per cell. Low density cell suspensions were associated with the highest seeding efficiency and uniformity of cell distribution, and the greatest increases in BMP‐2 production from 2 to 7 days after seeding. Our results highlight the potential for development of an optimized cell density and seeding technique that could greatly reduce the number of MSCs needed to produce therapeutic BMP‐2 levels in clinical situations. Further studies are needed to investigate in vivo effects of cell seeding techniques on bone healing.
Complications, Symptoms, Presurgical Predictors in Patients With Chronic Hypoparathyroidism: A Systematic Review
Authors: Yao Liang , Hui Xu , Li Meixuan , Li Jing , Ahmed Muhammad Muneeb , Lin Clement , Kandi Maryam , Sreekanta Ashwini , Makhdami Nima , Tamilselvan Divya , Ali Dalal S. , Dandurand Karel , Yang Kehu , Bilezikian John P. , Brandi Maria Luisa , Clarke Bart L. , Mannstadt Michael , Rejnmark Lars , Khan Aliya A. , Guyatt Gordon 
Published: August 2022

ABSTRACT
The complications and symptoms of hypoparathyroidism remain incompletely defined. Measuring serum parathyroid hormone (PTH) and calcium levels early after total thyroidectomy may predict the development of chronic hypoparathyroidism. The study aimed (i) to identify symptoms and complications associated with chronic hypoparathyroidism and determine the prevalence of those symptoms and complications (Part I), and (ii) to examine the utility of early postoperative measurements of PTH and calcium in predicting chronic hypoparathyroidism (Part II). We searched Medline, Medline In-Process, EMBASE, and Cochrane CENTRAL to identify complications and symptoms associated with chronic hypoparathyroidism. We used two predefined criteria (at least three studies reported the complication and symptom and had statistically significantly greater pooled relative estimates). To estimate prevalence, we used the median and interquartile range (IQR) of the studies reporting complications and symptoms. For testing the predictive values of early postoperative measurements of PTH and calcium, we used a bivariate model to perform diagnostic test meta-analysis. In Part I, the 93 eligible studies enrolled a total of 18,973 patients and reported on 170 complications and symptoms. We identified nine most common complications or symptoms probably associated with chronic hypoparathyroidism. The complications or symptoms and the prevalence are as follows: nephrocalcinosis/nephrolithiasis (median prevalence among all studies 15%), renal insufficiency (12%), cataract (17%), seizures (11%), arrhythmia (7%), ischemic heart disease (7%), depression (9%), infection (11%), and all-cause mortality (6%). In Part II, 18 studies with 4325 patients proved eligible. For PTH measurement, regarding the posttest probability, PTH values above 10 pg/mL 12–24 hours postsurgery virtually exclude chronic hypoparathyroidism irrespective of pretest probability (100%). When PTH values are below 10 pg/mL, posttest probabilities range from 3% to 64%. Nine complications and symptoms are probably associated with chronic hypoparathyroidism. A PTH value above a threshold of 10 pg/mL 12–24 hours after total thyroidectomy is a strong predictor that the patients will not develop chronic hypoparathyroidism. Patients with PTH values below the threshold need careful monitoring as some will develop chronic hypoparathyroidism. © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
Panchromatic oxasmaragdyrin as dual functional hole‐transporting material in all‐inorganic CsPbIBr2 perovskite solar cells
Authors: Leonardus Mario , Rameez Mohammad , Aji Bramantyo Bayu , Wu Wen‐Ti , Hung Chen‐Hsiung 
Published: August 2022

AbstractAll‐inorganic perovskite CsPbIBr2 offers both an optimal bandgap and good stability. The perovskite solar cells (PSCs) with CsPbIBr2 as a solar harvester utilize mainly spiro‐OMeTAD as a hole‐transporting material (HTM). Still, these PSCs are prone to degradation and not cost‐effective. Herein we examined, for the first time, a metal‐free dimethoxyboranyl (B(OR)2) chelated oxasmaragdyrin, SM‐OMe, as an alternative bifunctional HTM for CsPbIBr2‐based PSCs to demonstrate promising power conversion efficiencies at lower costs. The devices with SM‐OMe as HTM outperform spiro‐OMeTAD‐based devices with the highest power conversion efficiency of 6.04%. This enhanced performance can be attributed to an HTM and sensitizer dual‐function of SM‐OMe, which extends the absorption edge and consequently improves the short circuit current density. Furthermore, SM‐OMe‐based devices exhibited better charge extracting and hole‐transporting properties. Hence, this study demonstrates an effective and successful utilization of a new HTM with a bifunctional role as an alternative to conventional spiro‐OMeTAD in all‐inorganic PSCs.
Hsa_circ_0045932 regulates the progression of colorectal cancer by regulating HK2 through sponging miR‐873‐5p
Authors: Hong Feng , Deng Zhihua , Tie Ruxiu , Yang Senlin 
Published: August 2022

AbstractBackgroundCircular RNAs (circRNAs) have been confirmed to be key regulators for colorectal cancer (CRC) progression. The purpose of this research was to explore the biological role and mechanism of hsa_circ_0045932 in CRC.MethodsRT‐qPCR and Western blot (WB) were applied to examine RNA and protein levels, respectively. MTT assay, EdU assay, and transwell assay were used to detect cell proliferative, migration, and invasion. Glucose uptake and lactic acid level were determined to assess cellular glycolysis. Dual‐luciferase reporter and RIP assays were carried out to detect the relationship between miR‐873‐5p and hsa_circ_0045932 or hexokinase 2 (HK2). Xenograft mice model was established to confirm the function of hsa_circ_0045932 in vivo.ResultsHsa_circ_0045932 was overexpressed in CRC tissue samples and cells. Hsa_circ_0045932 knockdown repressed CRC cell proliferation, invasion, migration, and glycolysis abilities in vitro. MiR‐873‐5p could be sponged by hsa_circ_0045932, and its inhibitor also reversed the inhibitory effect of hsa_circ_0045932 knockdown on CRC cell progression. HK2 was targeted by miR‐873‐5p, and hsa_circ_0045932 regulated HK2 expression through targeting miR‐873‐5p. Overexpression of HK2 reversed the repressive effect of hsa_circ_0045932 knockdown on CRC cell malignant behaviors. Furthermore, the pro‐tumor role of hsa_circ_0045932 in vivo was also confirmed using animal experiments.ConclusionHsa_circ_0045932 promoted CRC progression through sponging miR‐873‐5p to up‐regulate HK2, which might offer novel therapeutic target for CRC clinical intervention.
Assessment of patient based real‐time quality control on comparative assays for common clinical analytes
Authors: Lu Yide , Yang Fan , Wen Dongmei , Shi Kaifeng , Gu Zhichao , Lu Qiuya , Wang Xuefeng , Dong Danfeng 
Published: August 2022

AbstractBackgroundIt is critical for laboratories to conduct multianalyzer comparisons as a part of daily routine work to strengthen the quality management of test systems. Here, we explored the application of patient‐based real‐time quality controls (PBRTQCs) on comparative assays to monitor the consistency among clinical laboratories.MethodsThe present study included 11 commonly tested analytes that were detected using three analyzers. PBRTQC procedures were set up with exponentially weighted moving average (EWMA) algorithms and evaluated using the AI‐MA artificial intelligence platform. Comparative assays were carried out on serum samples, and patient data were collected. Patients were divided into total patient (TP), inpatient (IP), and outpatient (OP) groups.ResultsOptimal PBRTQC protocols were evaluated and selected with appropriate truncation limits and smoothing factors. Generally, similar comparative assay performance was achieved using both the EWMA and median methods. Good consistency between the results from patients' data and serum samples was obtained, and unacceptable bias was detected for alkaline phosphatase (ALP) and gamma‐glutamyl transferase (GGT) when using analyzer C. Categorizing patients' data and applying specific groups for comparative assays could significantly improve the performance of PBRTQCs. When monitoring the inter‐ and intraanalyzer stability on a daily basis, EWMA was superior in detecting very small quality‐related changes with lower false‐positive alarms.ConclusionsWe found that PBRTQCs have the potential to efficiently assess multianalyzer comparability. Laboratories should be aware of population variations concerning both analytes and analyzers to build more suitable PBRTQC protocols.
Platelet‐derived immuno‐inflammatory indices show best performance in early prediction of COVID‐19 progression
Authors: Milenkovic Jelena , Djindjic Boris , Djordjevic Branka , Stojiljkovic Vladana , Stojanovic Dijana , Petrovic Stana , Matejic Ivan 
Published: August 2022

AbstractBackgroundCoronavirus disease 2019 (COVID‐19) profoundly affects the immune and hematopoietic systems with various degrees of reactive changes in the blood cell counts. Immuno‐inflammatory indices are considered a simple and effective tool in the prediction of COVID‐19 outcomes. We aimed to evaluate and compare the usefulness of leukocyte and platelet counts‐based immuno‐inflammatory indices on admission to hospital in predicting COVID‐19 progression and mortality.MethodsA total of 945 patients were enrolled. In addition to blood cell counts, we assessed hemogram‐derived immuno‐inflammatory indices in relation to COVID‐19 progression and death. The indices were tested by analysis of variance, receiver operating characteristic curve analysis, and binomial logistic regressions.ResultsPatients with severe COVID‐19 had significantly higher counts of neutrophils, eosinophils, and large immature cells (LIC), while decreased counts of platelets and monocytes. Lymphopenia was found in all of the patients, but without significant association with the outcomes. Patients with a LIC count ≥0.265 x 09/L had 54.7% more odds of having COVID‐19 progression. In multivariable analyses, platelets/neutrophil‐to‐lymphocyte ratio (P/NLR) and platelets‐to‐neutrophil radio (P/N) were significant independent predictors of COVID‐19 progression and mortality. The odds of a poor outcome were two times higher in cases with P/NLR < 43 x 109/L and P/N < 29 x 109/L.ConclusionIndices that include platelet count in combination with neutrophil and/or lymphocyte counts displayed the best discriminatory ability and prognostic value of COVID‐19 outcomes. Additionally, LIC showed promising results in the early identification of severe COVID‐19.
Identification of novel compound heterozygous mutations of the MYO15A gene with autosomal recessive non‐syndromic hearing loss
Authors: Wang Luming , Zhang Yue , Xue Qiuxia , Huang Pinghua , Liu Xiaodan 
Published: August 2022

AbstractBackgroundThe most common inheritance pattern responsible for congenital deafness belongs to autosomal recessive non‐syndromic hearing loss (ARNSHL) and mutations of the highly heterogeneous MYO15A locus are present in a large proportion of cases.MethodsOne Chinese family with ARNSHL was subjected to clinical evaluation and genetic analysis. We used targeted and whole exome sequencing with Sanger sequencing to identify and characterize mutations. Bioinformatics analysis was conducted to evaluate molecular functions.ResultsThree compound heterozygous MYO15A gene variants, including two novel variants, c.6804G > A (p.M2268I), and c.6188_6190delinsGTCA (p.F2063Cfs*60), responsible for deafness were identified. Pathogenicity was assessed by multiple bioinformatics analyses.ConclusionWe identified novel mutations of the MYO15A locus associated with ARNSHL in a Chinese family. The current findings expand the MYO15A pathogenic mutation spectrum to assist with genetic counseling and prenatal diagnosis.
No excuses, no exceptions except with compassion: A narrative review of visitor aggression in pediatrics
Authors: Jawed Aysha , Thompson Bernadette Simone 
Published: August 2022

AbstractVisitor aggression in pediatrics is a form of workplace violence that has not received much focus across pediatric health care systems. There are many different practices, strategies, and policies implemented across children's hospitals to mitigate visitor aggression on both clinical and organizational levels. To date, there has not been a published review on interventions in pediatrics to de‐escalate the intensity of these circumstances in the context of providing care and treatment to children. The goals of this review are to identify and assess the efficacy of each effort implemented across diverse pediatric health care settings. This review also proposes research, organizational, clinical, and policy implications and considerations that could help inform future practice in hospital‐based violence prevention. A narrative review of the literature identified eleven peer‐reviewed studies that delineated practices, strategies, and educational efforts between 1994 and 2020. All studies were descriptive in nature. There were four cross‐sectional studies, three qualitative studies, one mixed‐methods study, two prospective studies (one of which also had a group comparison design), and one non‐experimental study. The primary measures implemented across children's hospitals involved increasing security and police presence in patient care areas (n = 3), development of multidisciplinary workgroups to review and revise visitation policies (n = 2), crisis response teams (n = 2), and incident reporting systems (n = 2). Hospital risk management, administration, and clinical leadership teams were stakeholders involved in designing interventions across studies. Hospitals varied in allocation of support resources and in defining visitor aggression which contributed to mixed findings across studies.
Impact of pregnancy planning and preconceptual dietary training on metabolic control and offspring's outcome in phenylketonuria
Authors: Grohmann‐Held Karina , Burgard Peter , Baerwald Christoph G. O. , Beblo Skadi , vom Dahl Stephan , Das Anibh , Dokoupil Katharina , Fleissner Sandra , Freisinger Peter , Heddrich‐Ellerbrok Margret , Jung Alexandra , Korpel Vanessa , Krämer Johannes , Lier Dinah , Maier Esther M. , Meyer Uta , Mühlhausen Chris , Newger Martha , Och Ulrike , Plöckinger Ursula , Rosenbaum‐Fabian Stefanie , Rutsch Frank , Santer René , Schick Petra , Schwarz Martin , Spiekerkötter Ute , Strittmatter Ursula , Thiele Alena G. , Ziagaki Athanasia , Mütze Ulrike , Gleich Florian , Garbade Sven F. , Kölker Stefan 
Published: August 2022

AbstractTo prevent maternal phenylketonuria (PKU) syndrome low phenylalanine concentrations (target range, 120–360 μmol/L) during pregnancy are recommended for women with PKU. We evaluated the feasibility and effectiveness of current recommendations and identified factors influencing maternal metabolic control and children's outcome. Retrospective study of first successfully completed pregnancies of 85 women with PKU from 12 German centers using historical data and interviews with the women. Children's outcome was evaluated by standardized IQ tests and parental rating of child behavior. Seventy‐four percent (63/85) of women started treatment before conception, 64% (54/85) reached the phenylalanine target range before conception. Pregnancy planning resulted in earlier achievement of the phenylalanine target (18 weeks before conception planned vs. 11 weeks of gestation unplanned, p < 0.001) and lower plasma phenylalanine concentrations during pregnancy, particularly in the first trimester (0–7 weeks of gestation: 247 μmol/L planned vs. 467 μmol/L unplanned, p < 0.0001; 8–12 weeks of gestation: 235 μmol/L planned vs. 414 μmol/L unplanned, p < 0.001). Preconceptual dietary training increased the success rate of achieving the phenylalanine target before conception compared to women without training (19 weeks before conception vs. 9 weeks of gestation, p < 0.001). The majority (93%) of children had normal IQ (mean 103, median age 7.3 years); however, IQ decreased with increasing phenylalanine concentration during pregnancy. Good metabolic control during pregnancy is the prerequisite to prevent maternal PKU syndrome in the offspring. This can be achieved by timely provision of detailed information, preconceptual dietary training, and careful planning of pregnancy.
Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarumLP90 against dextran sulfate sodium‐induced colitis in mice
Authors: Xue Zihan , Li Ruilin , Liu Junyu , Zhou Jingna , Zhang Xiaoyu , Zhang Tingting , Zhang Min , Yang Yang , Chen Haixia 
Published: August 2022

AbstractBACKGROUNDSoluble dietary fiber (SDF) obtained from Lentinula edodes byproducts has beneficial effects on human intestinal health. This study aimed to examine the combined preventive and ameliorative effects of a kind of synbiotic (SDF with a molecular weight of 1.58 × 102 kDa and Lactobacillus plantarum LP90 (LP) at 1 × 109 CFU kg−1) on dextran sulfate sodium‐induced colitis mice.RESULTSThe results demonstrated that synbiotic treatment could alleviate weight loss, decrease the disease activity index level and cause histological amelioration. Synbiotic treatment also promoted the production of goblet cells, increased the expression of tight junction proteins, and adjusted the production of myeloperoxidase, malondialdehyde and superoxide dismutase to repair intestinal epithelial injury. Clinical symptoms were alleviated by maintaining Th17/Treg balance, increasing interleukin 10 and immunoglobulin A levels, reducing interleukin 17a and tumor necrosis factor α production, and promoting mRNA to highly express of Foxp3 and vitamin D receptors. Moreover, synbiotic treatment could upregulate butyric acid production (4.71 ± 0.46 mol g−1 feces, P < 0.05) and diversity of intestinal microbial to maintain intestinal homeostasis.CONCLUSIONThis study suggested that the combination of LP and SDF as a synbiotic has the potential for use as a nutritional supplement to alleviate colitis. © 2022 Society of Chemical Industry.