bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arteriovenous malformation of small intestine successfully treated by double-balloon enteroscopy and laparoscope-assisted surgery
Authors: Tohma Takayuki , Okabe Yasuyuki , Ushio Masaya , Saito Masaya 
Published: December 2022

Abstract
Arteriovenous malformation (AVM) of the small bowel is a rare disease and can be sometimes difficult to treat due to the diagnostic difficulty. We herein report a case of small intestinal bleeding of AVM successfully treated with double-balloon enteroscopy (DBE) and laparoscope-assisted resection. A 44-year-old man complained of hematochezia and visited the previous doctor. He underwent gastroscopy and colonoscopy, but no bleeding site was detected. However, he rebled 2 days later and became hypotensive. Abdominal computed tomography revealed a hypervascular nodule in the jejunum. He was transferred to our institution for further treatment. DBE was performed and revealed a small pulsatile lesion with a tiny mucosal break. We then injected a marking tattoo. Two days later, he underwent an operation. We were able to easily locate the tattooed lesion laparoscopically and performed jejunal partial resection. His postoperative course was uneventful. DBE enabled a precise diagnosis and minimal invasive surgery.
Online Screening and Personalized Education to Identify Post-Deployment Mental Health Need and Facilitate Access to Care
Authors: Mengeling Michelle A , Torner James C , Smith Jeffrey L , Cook Brian L , Sadler Anne G 
Published: December 2022

ABSTRACT

Introduction
Reserve and National Guard (RNG) service members face increased risks for psychological and behavioral problems and are unlikely to seek mental health (MH) care after returning from military deployments. This article examines an online intervention (Web-Ed) with regard to participation, screening results, satisfaction, and intent to seek follow-up MH care, with comparisons by gender and post-deployment MH care receipt.

Materials and Methods
This was a cross-sectional study of 414 RNG service members (214 women and 200 men), who returned from deployments to or in support of the Iraq or Afghanistan wars within the prior 36 months. Participants completed Web-Ed, which includes screening, personalized education, and links to Veterans Health Administration health care.

Results
Positive post-deployment screening proportions, Web-Ed satisfaction, and intent to seek follow-up care were similar for men and women. Few had received MH care (33% women; 24% men), yet most screened positive on at least one screen (69% women; 72% men). Most indicated that they would recommend Web-Ed to other veterans (71% women; 67% men) and that they received useful information they may not have received otherwise (52% women; 53% men) regardless of gender or prior MH care. Almost half (40% women; 48% men) planned to seek further assessment from a health care provider as a direct result of Web-Ed.

Conclusions
Efforts to facilitate access to MH care among post-deployed RNG veterans should be ongoing, regardless of the length of time since deployment, Veterans Affairs enrollment status, prior MH care receipt, or gender. Online screening and personalized education engage veterans who have not sought MH care and provide new information to those who have.
Prospects for detecting transient quasi-monochromatic gravitational waves from glitching pulsars with current and future detectors
Authors: Moragues Joan , Modafferi Luana M , Tenorio Rodrigo , Keitel David 
Published: December 2022

ABSTRACT
Pulsars are rotating neutron stars that emit periodic electromagnetic radiation. While pulsars generally slow down as they lose energy, some also experience glitches: spontaneous increases of their rotational frequency. According to several models, these glitches can also lead to the emission of long-duration transient gravitational waves (GWs). We present detection prospects for such signals by comparing indirect energy upper limits on GW strain for known glitches with the sensitivity of current and future ground-based GW detectors. We first consider the optimistic case of generic constraints based on the glitch size and find that realistic matched-filter searches in the fourth LIGO–Virgo–KAGRA observing run (O4) could make a detection, or set constraints below these indirect upper limits, for equivalents of 36 out of 726 previously observed glitches, and 74 in the O5 run. With the third-generation Einstein Telescope or Cosmic Explorer, 35–40 per cent of glitches would be accessible. When specializing to a scenario where transient mountains produce the post-glitch GW emission, following the Yim & Jones model, the indirect upper limits are stricter. Out of the smaller set of 119 glitches with measured healing parameter, as needed for predictions under that model, only 6 glitches would have been within reach for O4 and 14 for O5, with a similar percentage as before with third-generation detectors. We also discuss how this model matches the observed glitch population.
A search for variable subdwarf B stars in TESS Full Frame Images III. An update on variable targets in both ecliptic hemispheres – contamination analysis and new sdB pulsators
Authors: Sahoo S K , Baran A S , Worters H L , Németh P , Kilkenny D 
Published: December 2022

ABSTRACT
We present an update on the variable star survey performed on the TESS 30 min Full Frame Image (FFI) data reported by our first two papers in this series. This update includes a contamination analysis in order to identify false positives and analysis of the TESS 10 min FFI data collected during Years 3 and 4 of the mission. We clarify the variability status of 2 995 targets identifying 1 403 variable stars. In addition, we spectroscopically classify 24 pre-filtered targets sampled with the 10 min FFI data and discover 11 new sdB pulsators. Future follow-up space- and/or ground-based data of variables reported here, to identify the nature of their variability and reveal spectroscopic parameters of the stars, would complement this work.
Model selection using time-delay lenses
Authors: Melia Fulvio , Wei Jun-Jie , Wu Xue-Feng 
Published: December 2022

ABSTRACT
The sample of time-delay gravitational lenses appropriate for studying the geometry of the Universe continues to grow as dedicated campaigns, such as the Dark Energy Survey, the (Very Large Telescope) VLT Survey Telescope (VST) ATLAS survey, and the Large Synoptic Survey Telescope, complete their census of high-redshift sources. This catalogue now includes hundreds of strong lensing systems, at least 31 of which have reasonably accurate time-delay measurements. In this paper, we use them to compare the predictions of two competing Friedmann–Lemaître–Robertson–Walker models: flat Lambda cold dark matter (ΛCDM), characterized by two adjustable parameters (H0 and Ωm), and the Rh = ct universe (with H0 as the single free variable). Over the past decade, the latter has accounted for the data better than the standard model, most recently the emergence of well-formed galaxies discovered by JWST at cosmic dawn. Here, we show that the current sample of time-delay lenses favours Rh = ct with a likelihood of ∼$84{{\ \rm per\ cent}}$ versus ∼$16{{\ \rm per\ cent}}$ for the standard model. This level of accuracy will greatly improve as the ongoing surveys uncover many thousands of additional lens systems over the next several years.
CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids
Authors: Kim Seong Keun , Kim Haseong , Woo Seung Gyun , Kim Tae Hyun , Rha Eugene , Kwon Kil Koang , Lee Hyewon , Lee Seung-Goo , Lee Dae-Hee 
Published: December 2022

Abstract
Antibiotics have been widely used for plasmid-mediated cell engineering. However, continued use of antibiotics increases the metabolic burden, horizontal gene transfer risks, and biomanufacturing costs. There are limited approaches to maintaining multiple plasmids without antibiotics. Herein, we developed an inverter cascade using CRISPRi by building a plasmid containing a single guide RNA (sgRNA) landing pad (pSLiP); this inhibited host cell growth by repressing an essential cellular gene. Anti-sgRNAs on separate plasmids restored cell growth by blocking the expression of growth-inhibitory sgRNAs in pSLiP. We maintained three plasmids in Escherichia coli with a single antibiotic selective marker. To completely avoid antibiotic use and maintain the CRISPRi-based logic inverter cascade, we created a novel d-glutamate auxotrophic E. coli. This enabled the stable maintenance of the plasmid without antibiotics, enhanced the production of the terpenoid, (−)-α-bisabolol, and generation of an antibiotic-resistance gene-free plasmid. CRISPRi is therefore widely applicable in genetic circuits and may allow for antibiotic-free biomanufacturing.
Genome-scale top-down strategy to generate viable genome-reduced phages
Authors: Yuan Shengjian , Shi Juan , Jiang Jianrong , Ma Yingfei 
Published: December 2022

Abstract
Efforts have been made to reduce the genomes of living cells, but phage genome reduction remains challenging. It is of great interest to investigate whether genome reduction can make phages obtain new infectious properties. We developed a CRISPR/Cas9-based iterative phage genome reduction (CiPGr) approach and applied this to four distinct phages, thereby obtaining heterogeneous genome-reduced mutants. We isolated and sequenced 200 mutants with loss of up to 8–23% (3.3–35 kbp) of the original sequences. This allowed the identification of non-essential genes for phage propagation, although loss of these genes is mostly detrimental to phage fitness to various degrees. Notwithstanding this, mutants with higher infectious efficiency than their parental strains were characterized, indicating a trade-off between genome reduction and infectious fitness for phages. In conclusion, this study provides a foundation for future work to leverage the information generated by CiPGr in phage synthetic biology research.
Changes in outcomes and factors associated with survival in melanoma patients with brain metastases
Authors: Hasanov Merve , Milton Denái R , Davies Alicia Bea , Sirmans Elizabeth , Saberian Chantal , Posada Eliza L , Opusunju Sylvia , Gershenwald Jeffrey E , Torres-Cabala Carlos A , Burton Elizabeth M , Colen Rivka R , Huse Jason T , Glitza Oliva Isabella C , Chung Caroline , McAleer Mary Frances , McGovern Susan L , Yeboa Debra N , Kim Betty Y S , Prabhu Sujit S , McCutcheon Ian E , Weinberg Jeffrey S , Lang Frederick F , Tawbi Hussein A , Li Jing , Haydu Lauren E , Davies Michael A , Ferguson Sherise D 
Published: December 2022

Abstract

Background
Treatment options for patients with melanoma brain metastasis (MBM) have changed significantly in the last decade. Few studies have evaluated changes in outcomes and factors associated with survival in MBM patients over time. The aim of this study is to evaluate changes in clinical features and overall survival (OS) for MBM patients.

Methods
Patients diagnosed with MBMs from 1/1/2009 to 12/31/2013 (Prior Era; PE) and 1/1/2014 to 12/31/2018 (Current Era; CE) at The University of Texas MD Anderson Cancer Center were included in this retrospective analysis. The primary outcome measure was OS. Log-rank test assessed differences between groups; multivariable analyses were performed with Cox proportional hazards models and recursive partitioning analysis (RPA).

Results
A total of 791 MBM patients (PE, n = 332; CE, n = 459) were included in analysis. Median OS from MBM diagnosis was 10.3 months (95% CI, 8.9–12.4) and improved in the CE vs PE (14.4 vs 10.3 months, P &lt; .001). Elevated serum lactate dehydrogenase (LDH) was the only factor associated with worse OS in both PE and CE patients. Factors associated with survival in CE MBM patients included patient age, primary tumor Breslow thickness, prior immunotherapy, leptomeningeal disease, symptomatic MBMs, and whole brain radiation therapy. Several factors associated with OS in the PE were not significant in the CE. RPA demonstrated that elevated serum LDH and prior immunotherapy treatment are the most important determinants of survival in CE MBM patients.

Conclusions
OS and factors associated with OS have changed for MBM patients. This information can inform contemporary patient management and clinical investigations.
A universal training scheme and the resulting universality for machine learning phases
Authors: Tseng Yuan-Heng , Jiang Fu-Jiun , Huang C-Y 
Published: December 2022

Abstract
An autoencoder (AE) and a generative adversarial network (GAN) are trained only once on a one-dimensional (1D) lattice of 200 sites. Moreover, the AE contains only one hidden layer consisting of two neurons, and both the generator and the discriminator of the GAN are made up of two neurons as well. The training set employed to train both the considered unsupervised neural networks (NNs) is composed of two artificial configurations. Remarkably, despite their simple architectures, both the built AE and GAN have precisely determined the critical points of several models, including the three-dimensional classical O(3) model, the two-dimensional generalized classical XY model, the two-dimensional two-state Potts model, and the one-dimensional Bose–Hubbard model. In addition, a factor of several thousands in the speed of calculation is gained for the built AE and GAN when they are compared with the conventional unsupervised NN approaches. The results presented here, as well as those shown previously in the literature, suggest that when phase transitions are considered, an elegant universal neural network that is extremely efficient and is applicable to broad physical systems can be constructed with ease. In particular, since an NN trained with two configurations can be applied to many models, it is likely that when machine learning is concerned, the majority of phase transitions belong to a class having two elements, i.e. the Ising class.
Impact of COVID-19 pandemic on the management of patients with RA: a survey of rheumatologists in six European countries
Authors: Machado Pedro M , Verschueren Patrick , Grainger Rebecca , Jones Hannah , Piercy James , van Beneden Katrien , Caporali Roberto , Dejaco Christian , Fautrel Bruno 
Published: December 2022

AbstractObjectiveWe aimed to describe, from the perspective of rheumatologists in Europe, how the coronavirus disease 2019 (COVID-19) pandemic has impacted their management of people with RA and the continuing medical education of physicians.MethodsRheumatologists participating in the Adelphi RA Disease Specific ProgrammeTM in six European countries were contacted in August and September 2020 for a telephone survey. Rheumatologists were asked seven attitudinal questions on changes to patient management, prescription behaviour and continuing education owing to COVID-19. Results were summarized with descriptive statistics.ResultsThe telephone survey was completed by 284 rheumatologists. The most commonly reported changes to patient management were increased utilization of video/telephone consultations (66.5% of respondents), fewer visits (58.5%) and limiting physical contact (58.1%). Furthermore, 67.9% of rheumatologists who indicated that prescribing behaviour had changed switched their patients to self-administered medication, and 60.7% reported not starting patients on targeted synthetic DMARDs, biologic originator DMARDs or biosimilar DMARDs. In total, 57.6% of rheumatologists believed that changes in management would persist. Rheumatologists reported that 38.0% of patients expressed concerns about how COVID-19 would impact treatment, including access to treatment and the risk of infection. The biggest impact on rheumatologist education was a switch to online training and conferences.ConclusionAll countries saw changes in patient management and prescribing behaviour, including the rapid uptake of telemedicine. It is important that the international rheumatology community learns from these experiences to prepare better for future pandemics and to address ongoing rheumatologist shortages.
Modified fibrin hydrogel for sustained delivery of RNAi lipopolyplexes in skeletal muscle
Authors: Ngarande Ellen , Doubell Emma , Tamgue Ousman , Mano Manuel , Human Paul , Giacca Mauro , Davies Neil Hamer 
Published: December 2022

Abstract
RNA interference is a promising therapeutical approach presently hindered by delivery concerns such as rapid RNA degradation and targeting of individual tissues. Injectable hydrogels are one potentially simple and direct route towards overcoming these barriers. Here we report on the utility of a combination of a mildly modified form of the clinically utilised fibrin hydrogel with Invivofectamine® 3.0, a lipid nonviral transfection vector, for local and sustained release. PEGylation of fibrin allowed for controlled release of small interfering RNA (siRNA)-lipopolyplexes for at least 10 days and greatly increased the stability of fibrin in vitro and in vivo. A 3D cell culture model and a release study showed transfection efficacy of siRNA-lipopolyplexes was retained for a minimum of 7 days. Injection in conjunction with PEGylated-fibrinogen significantly increased retention of siRNA-lipopolyplexes in mouse skeletal muscle and enhanced knockdown of myostatin mRNA that correlated with muscle growth. Thus, the increased efficacy observed here for the combination of a lipid nanoparticle, the only type of nonviral vector approved for the clinic, with fibrin, might allow for more rapid translation of injectable hydrogel-based RNA interference.
The Eleventh-Century ‘N’ Psalter from England: New Pieces of the Puzzle
Authors: Opalińska Monika , Pludra-Żuk Paulina , Chlebus Ewa 
Published: December 2022

Abstract
This paper presents an analysis of two recently found fragments of a Latin psalter with a continuous Old English gloss. The fragments were used as endleaf guards in an early modern book from the collection of Samuel Meienreis, currently held at the C. Norwid Library in Elbląg. We argue that the newly found parchment pieces match four other membra disiecta from the same eleventh-century codex produced in England. Since the formerly identified fragments were removed from the bindings of unknown books, their provenance and the origin of the manuscript from which they were removed have not been established, so far. The new findings partially fill this gap. In this paper we explore palaeographical and linguistic evidence, and the historical context of the manuscript waste found in Elbląg in an attempt to reconstruct the history of the so-called N Psalter to which all the extant pieces once belonged.
Hazed and Confused: The Effect of Air Pollution on Dementia
Authors: Bishop Kelly C , Ketcham Jonathan D , Kuminoff Nicolai V 
Published: December 2022

Abstract
We study whether long-term cumulative exposure to airborne small particulate matter (PM2.5) affects the probability that an individual receives a new diagnosis of Alzheimer's disease or related dementias. We track the health, residential location, and PM2.5 exposures of Americans aged sixty-five and above from 2001 through 2013. The expansion of Clean Air Act regulations led to quasi-random variation in individuals’ subsequent exposures to PM2.5. We leverage these regulations to construct instrumental variables for individual-level decadal PM2.5 that we use within flexible probit models that also account for any potential sample selection based on survival. We find that a 1 µg/m3 increase in decadal PM2.5 increases the probability of a new dementia diagnosis by an average of 2.15 percentage points (pp). All else equal, we find larger effects for women, older people, and people with more clinical risk factors for dementia. These effects persist below current regulatory thresholds.
Serum IFNα2 levels are associated with disease activity and outperform IFN-I gene signature in a longitudinal childhood-onset SLE cohort
Authors: Wahadat M Javad , Qi Hongchao , van Helden-Meeuwsen Cornelia G , Huijser Erika , van den Berg Lotte , van Dijk-Hummelman Annette , Göpfert Jens C , Heine Anne , Verkaaik Marleen , Schreurs Marco W J , Dik Willem A , Kamphuis Sylvia , Versnel Marjan A 
Published: December 2022

Abstract

Objective
To study the association of serum IFNα2 levels measured by ultrasensitive single-molecule array (Simoa) and the IFN-I gene signature (IGS) with disease activity and determine whether these assays can mark disease activity states in a longitudinal cohort of childhood-onset SLE (cSLE) patients.

Methods
Serum IFNα2 levels were measured in 338 samples from 48 cSLE patients and 67 healthy controls using an IFNα Simoa assay. Five-gene IGS was measured by RT-PCR in paired whole blood samples. Disease activity was measured by clinical SELENA-SLEDAI and BILAG-2004. Low disease activity was defined by Low Lupus Disease Activity State (LLDAS) and flares were characterized by SELENA-SLEDAI flare index. Analysis was performed using linear mixed models.

Results
A clear positive correlation was present between serum IFNα2 levels and the IGS (r = 0.78, P &lt; 0.0001). Serum IFNα2 levels and IGS showed the same significant negative trend in the first 3 years after diagnosis. In this timeframe, mean baseline serum IFNα2 levels decreased by 55.1% (Δ 201 fg/ml, P &lt; 0.001) to a mean value of 164 fg/ml, which was below the calculated threshold of 219.4 fg/ml that discriminated between patients and healthy controls. In the linear mixed model, serum IFNα2 levels were significantly associated with both cSELENA-SLEDAI and BILAG-2004, while the IGS did not show this association. Both IFN-I assays were able to characterize LLDAS and disease flare in receiver operating characteristic analysis.

Conclusions
Serum IFNα2 levels measured by Simoa technology are associated with disease activity scores and characterize disease activity states in cSLE.
Atypical hypnotic compound ML297 restores sleep architecture immediately following emotionally valenced learning, to promote memory consolidation and hippocampal network activation during recall
Authors: Martinez Jessy D , Brancaleone William P , Peterson Kathryn G , Wilson Lydia G , Aton Sara J 
Published: December 2022

AbstractSleep plays a critical role in consolidating many forms of hippocampus-dependent memory. While various classes of hypnotic drugs have been developed in recent years, it remains unknown whether, or how, some of them affect sleep-dependent memory consolidation mechanisms. We find that ML297, a recently developed candidate hypnotic agent targeting a new mechanism (activating GIRK1/2-subunit containing G-protein coupled inwardly rectifying potassium [GIRK] channels), alters sleep architecture in mice over the first 6 hr following a single-trial learning event. Following contextual fear conditioning (CFC), ML297 reversed post-CFC reductions in NREM sleep spindle power and REM sleep amounts and architecture, renormalizing sleep features to what was observed at baseline, prior to CFC. Renormalization of post-CFC REM sleep latency, REM sleep amounts, and NREM spindle power were all associated with improved contextual fear memory (CFM) consolidation. We find that improvements in CFM consolidation due to ML297 are sleep-dependent, and are associated with increased numbers of highly activated dentate gyrus (DG), CA1, and CA3 neurons during CFM recall. Together our findings suggest that GIRK1/2 channel activation restores normal sleep architecture— including REM sleep, which is normally suppressed following CFC—and increases the number of hippocampal neurons incorporated into the CFM engram during memory consolidation.