bluuebunny/crossref_metadata_2025_split huggingface dataset:
A visualization reporter system for characterizing antibiotic biosynthetic gene clusters expression with high-sensitivity
Authors: Liu Xiang , Li Jine , Li Yue , Li Junyue , Sun Huiying , Zheng Jiazhen , Zhang Jihui , Tan Huarong 
Published: September 2022

AbstractThe crisis of antibiotic resistance has become an impending global problem. Genome sequencing reveals that streptomycetes have the potential to produce many more bioactive compounds that may combat the emerging pathogens. The existing challenge is to devise sensitive reporter systems for mining valuable antibiotics. Here, we report a visualization reporter system based on Gram-negative bacterial acyl-homoserine lactone quorum-sensing (VRS-bAHL). AHL synthase gene (cviI) of Chromobacterium violaceum as reporter gene is expressed in Gram-positive Streptomyces to synthesize AHL, which is detected with CV026, an AHL deficient mutant of C. violaceum, via its violacein production upon AHL induction. Validation assays prove that VRS-bAHL can be widely used for characterizing gene expression in Streptomyces. With the guidance of VRS-bAHL, a novel oxazolomycin derivative is discovered to the best of our knowledge. The results demonstrate that VRS-bAHL is a powerful tool for advancing genetic regulation studies and discovering valuable active metabolites in microorganisms.
Pigment epithelium-derived factor promotes peritoneal dissemination of ovarian cancer through induction of immunosuppressive macrophages
Authors: Ueno Sayaka , Sudo Tamotsu , Saya Hideyuki , Sugihara Eiji 
Published: September 2022

AbstractPeritoneal dissemination of ovarian cancer (OC) correlates with poor prognosis, but the mechanisms underlying the escape of OC cells from the intraperitoneal immune system have remained unknown. We here identify pigment epithelium–derived factor (PEDF) as a promoting factor of OC dissemination, which functions through induction of CD206+ Interleukin-10 (IL-10)–producing macrophages. High PEDF gene expression in tumors is associated with poor prognosis in OC patients. Concentrations of PEDF in ascites and serum are significantly higher in OC patients than those with more benign tumors and correlated with early recurrence of OC patients, suggesting that PEDF might serve as a prognostic biomarker. Bromodomain and extraterminal (BET) inhibitors reduce PEDF expression and limit both OC cell survival and CD206+ macrophage induction in the peritoneal cavity. Our results thus implicate PEDF as a driver of OC dissemination and identify a BET protein–PEDF–IL-10 axis as a promising therapeutic target for OC.
Mitochondrial Ca2+ uptake by the MCU facilitates pyramidal neuron excitability and metabolism during action potential firing
Authors: Groten Christopher J. , MacVicar Brian A. 
Published: September 2022

AbstractNeuronal activation is fundamental to information processing by the brain and requires mitochondrial energy metabolism. Mitochondrial Ca2+ uptake by the mitochondrial Ca2+ uniporter (MCU) has long been implicated in the control of energy metabolism and intracellular Ca2+ signalling, but its importance to neuronal function in the brain remains unclear. Here, we used in situ electrophysiology and two-photon imaging of mitochondrial Ca2+, cytosolic Ca2+, and NAD(P)H to test the relevance of MCU activation to pyramidal neuron Ca2+ signalling and energy metabolism during action potential firing. We demonstrate that mitochondrial Ca2+ uptake by the MCU is tuned to enhanced firing rate and the strength of this relationship varied between neurons of discrete brain regions. MCU activation promoted electron transport chain activity and chemical reduction of NAD+ to NADH. Moreover, Ca2+ buffering by mitochondria attenuated cytosolic Ca2+ signals and thereby reduced the coupling between activity and the slow afterhyperpolarization, a ubiquitous regulator of excitability. Collectively, we demonstrate that the MCU is engaged by accelerated spike frequency to facilitate neuronal activity through simultaneous control of energy metabolism and excitability. As such, the MCU is situated to promote brain functions associated with high frequency signalling and may represent a target for controlling excessive neuronal activity.
Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion
Authors: Büttner Carina R. , Spurný Radovan , Füzik Tibor , Plevka Pavel 
Published: September 2022

AbstractCoxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous structural studies, and it was speculated that the virus is unique among enteroviruses in using altered particles with expanded capsids to infect cells. In contrast, the virions of other enteroviruses are required for infection. Here we used cryo-electron microscopy (cryo-EM) to determine the structures of the CV-A6 virion, altered particle, and empty capsid. We show that the CV-A6 virion has features characteristic of virions of other enteroviruses, including a compact capsid, VP4 attached to the inner capsid surface, and fatty acid-like molecules occupying the hydrophobic pockets in VP1 subunits. Furthermore, we found that in a purified sample of CV-A6, the ratio of infectious units to virions is 1 to 500. Therefore, it is likely that virions of CV-A6 initiate infection, like those of other enteroviruses. Our results provide evidence that future vaccines against CV-A6 should target its virions instead of the antigenically distinct altered particles. Furthermore, the structure of the virion provides the basis for the rational development of capsid-binding inhibitors that block the genome release of CV-A6.
An intermediate polar candidate toward the Galactic plane
Authors: Mondal S. , Ponti G. , Haberl F. , Anastasopoulou K. , Campana S. , Mori K. , Hailey C. J. , Rea N. 
Published: September 2022

Context. For the past decade, it has been suggested that intermediate polars (IPs), a subclass of magnetic cataclysmic variables (CVs), are one of the main contributors to the hard diffuse X-ray emission from the Galactic center (GC) and Galactic ridge.
Aims. In our ongoing XMM-Newton survey of the central region of the Galactic disk (20° ×2°), we detected a persistent IP candidate, 1.7° away from the GC. In this work, we better characterize the behavior of this source by looking at the new and archival XMM-Newton data.
Methods. We performed a detailed X-ray spectral modeling of the source. Furthermore, we searched for X-ray pulsations in the light curve as well as its counterpart at other wavelengths.
Results. The XMM-Newton spectrum (0.8–10 keV) of the source is described by a partial covering collisionally ionized diffuse gas with plasma temperature kT = 15.7−3.6+20.9 keV. In addition, the spectrum shows the presence of iron lines at E = 6.44, 6.65, and 6.92 keV with equivalent widths of 194−70+89, 115−75+79, and 98−74+93 eV, respectively. The X-ray light curve shows a coherent modulation with a period of P = 432.44 ± 0.36 s, which we infer is the spin period of the white dwarf. The white dwarf mass estimated from fitting a physical model to the spectrum results in MWD = 1.05−0.21+0.16 M⊙. We were able to find a likely optical counterpart in the Gaia catalog with a G magnitude of 19.26, and the distance to the source derived from the measured Gaia parallax is ∼4.3 kpc.
Conclusions. We provide an improved source localization with subarcsec accuracy. The spectral modeling of the source indicates the presence of intervening circumstellar gas, which absorbs the soft X-ray photons. The measured equivalent width of the iron lines and the detection of the spin period in the light curve are consistent with those from IPs.
Non-negative Matrix Factorization for Dimensionality Reduction
Authors: Olaya Jbari , Otman Chakkor 
Published: September 2022

Abstract—What matrix factorization methods do is reduce the dimensionality of the data without losing any important information. In this work, we present the Non-negative Matrix Factorization (NMF) method, focusing on its advantages concerning other methods of matrix factorization. We discuss the main optimization algorithms, used to solve the NMF problem, and their convergence. The paper also contains a comparative study between principal component analysis (PCA), independent component analysis (ICA), and NMF for dimensionality reduction using a face image database.
Index Terms—NMF, PCA, ICA, dimensionality reduction.
Perspektivpapier „Zukunft
Präventionsforschung“: Koordinierte Forschung zu
Prävention und Gesundheitsförderung – aktuell und in der
Zukunft
Authors: Zeeb Hajo , Brandes Mirko , Bauer Ullrich , Forberger Sarah , Gelius Peter , Muellmann Saskia , Okan Orkan , Pfeifer Klaus , Renner Britta , Wright Michael 
Published: September 2022

ZusammenfassungVon 2014 bis 2022 fördert das BMBF fünf
Forschungsverbünde der Präventionsforschung und
Gesundheitsförderung, die ebenfalls über eine gemeinsame
Koordination verfügen (Forschungsnetzwerk
Primärprävention und Gesundheitsförderung,
www.fp2g.net). Die Forschenden haben eine Vielzahl relevanter
Forschungsergebnisse erarbeitet und Erkenntnisse über wesentliche
Aspekte der Präventionsforschung und Gesundheitsförderung
gewonnen. Die Forschung der Verbünde konzentrierte sich auf Grundlagen,
anwendungsrelevante Erkenntnisse und Umsetzungsbedingungen einer langfristig
angelegten Prävention und Gesundheitsförderung für
nicht-übertragbare Erkrankungen (NCD). Den Einschränkungen durch
die Pandemie ab 2020 konnte z.T. durch Integration von Corona-bezogenen
Forschungs- und Transferaktivitäten begegnet werden. Die Bedeutung von
Resilienz für den Umgang mit den vielfältigen gesundheitlichen
und sozialen Herausforderungen wird in Zeiten der Pandemie verstärkt
analysiert und diskutiert. Für die Präventionsforschung gewinnen
Forschungsfragen etwa zur besseren Implementierung von
Präventionsmaßnahmen durch digitale Werkzeuge
zusätzliche Bedeutung. Gemeinsam stellen die Verbünde Erreichtes
und Desiderate zukünftiger Forschung dar. Dieses Perspektivpapier mit
den abschließend formulierten neun Thesen ist für eine
erfolgreiche Prävention und Gesundheitsförderung als
Diskussionsanregung für Förderer wie auch für die
Gemeinschaft der Forschenden gedacht. Es stellt sich explizit in die
Kontinuität der schon im Jahr 2012 erarbeiteten Memoranden zur
Präventionsforschung.
Das Persönlichkeitsinventar für ICD-11 (PiCD):
Reliabilität und Validität der Deutschen Version in einer
klinischen und nicht-klinischen Stichprobe
Authors: Damovsky Florian , Zettl Max , Zimmermann Johannes , Herbold Willy , Curtius Theresa , Bücker Susanne , Taubner Svenja , Volkert Jana 
Published: September 2022

ZusammenfassungMit der 11. Version der International Statistical Classification of Diseases and
Related Health Problems (ICD-11) erfolgt ein Paradigmenwechsel in der Diagnostik
von Persönlichkeitsstörungen: Deren Klassifikation erfolgt
künftig nicht mehr kategorial, sondern dimensional entlang der
Schweregrade von Persönlichkeitsbeeinträchtigungen und optional
der Präsenz maladaptiver Persönlichkeitsmerkmale. Diese Studie
untersucht die Reliabilität und Validität der deutschen Version
des Personality Inventory for ICD-11 (PiCD), ein Fragebogen zur Erfassung der
ICD-11 Persönlichkeitsdomänen, in einer klinischen und
nicht-klinischen Stichprobe (N=939). Die Faktorstruktur des PiCD wurde
mit Hilfe einer explorativen Faktorenanalyse (EFA) untersucht und zeigte eine
Tendenz für ein Vier-Faktoren Modell (Negative Affektivität,
Dissozialität, Distanziertheit und ein bipolarer Faktor
Enthemmung-Anankasmus). Die Subskalen des PiCD wiesen akzeptable bis exzellente
Reliabilitätskoeffizienten mit Cronbach‘s α=0,79
– 0,89 und McDonald‘s ω=0,76 – 0,90 auf.
Die konvergente und diskriminante Validität wurde anhand weiterer
Selbsteinschätzungsinstrumente untersucht und erwies sich als
zufriedenstellend. Die Ergebnisse legen nahe, dass es sich bei der deutschen
Version des PiCD um ein reliables und einigermaßen valides
Messinstrument zur Erfassung der maladaptiven Persönlichkeitsmerkmale
gemäß ICD-11 handelt. Es ist jedoch weitere Forschung zur
Faktorenstruktur, geeigneten Cut-Off- sowie Normwerten notwendig.
Familien mit erhöhtem elterlichen Stress und
Konfliktpotential: Eine Zielgruppe für psychosoziale
Unterstützungsangebote?
Authors: Ulrich Susanne Marlene , Renner Ilona , Lux Ulrike , Walper Sabine , Löchner Johanna 
Published: September 2022

Zusammenfassung
Ziel der Studie Psychosoziale Belastungen können die erste
Lebensphase von jungen Familien erschweren. Eine bisher wenig beleuchtete Gruppe
sind Familien mit erhöhtem elterlichen Stress und Konfliktpotential. In
diesem Beitrag sollen 1) die Kenntnis und Nutzung von
Unterstützungsangeboten elternstress- und konfliktbelasteter Familien
eingeordnet werden und 2) die Auswirkung von elterlichem Stress und
Konfliktpotential auf das Erziehungsverhalten untersucht werden.
Methodik Hierzu wurden Daten aus einer repräsentativen
Querschnittsstudie des Jahres 2015 mit N=7 549 Familien sowie der
Folgestudie mit n=905 Familien analysiert. Eltern, die mit ihrem Kind in
einer Kinderarztpraxis bei einer U3-U7a-Untersuchung waren, füllten
einen schriftlichen Fragebogen aus. Kenntnis und Nutzung der Angebote wurden bei
vier Belastungsgruppen (unbelastet, sozioökonomisch belastet,
elternstress- und konfliktbelastet und hochbelastet) untersucht.
Ergebnisse Familien mit erhöhtem elterlichem Stress und
Konfliktpotential nehmen trotz leicht erhöhter Kenntnis selektive
Angebote seltener in Anspruch als sozioökonomisch hochbelastete
Familien. Sie berichten häufiger dysfunktionales
Erziehungsverhalten.
Schlussfolgerung Dies wirft die Frage auf, ob Familien mit
erhöhtem elterlichen Stress und Konfliktpotential zu wenig
Unterstützung erhalten, weil sie keinen deutlich sichtbaren Hilfebedarf
haben oder ob sie aufgrund sozioökonomischer Ressourcen, der hohen
Angebotskenntnis und der Nutzung universeller medizinischer und
Familienbildungsangeboten ausreichend versorgt sind. Die Ergebnisse liefern
wichtige Hinweise für die Versorgung von Familien in unterschiedlichen
Belastungslagen und tragen dazu bei, den Unterstützungsbedarf
einzuschätzen.
Anforderungen an die heilberufliche Stellungnahme nach § 35a SGB VIII und Anregungen zur Umsetzung
Authors: Hiller Therese , Esch Lena-Maria , Gindele Verena , Böge Isabel , Witt Andreas , Fegert Jörg M. 
Published: September 2022

ZUSAMMENFASSUNGLegislative Neuerungen und damit verbundene Veränderungen in der Praxis machen eine Anpassung der heilberuflichen Stellungnahme nach § 35a SGB VIII erforderlich. Der vorliegende Artikel bietet einen Vorschlag zur Anpassung der heilberuflichen Stellungnahme. Berücksichtigung finden dabei die gesetzlichen Neuerungen durch das Kinder- und Jugendstärkungsgesetz sowie das Bundesteilhabegesetz, außerdem die in Kraft tretende International Classification of Disease-11 (ICD-11) und die International Classification of Functioning, Disability and Health (ICF).Die Anpassung der heilberuflichen Stellungnahme bietet die Diagnostik sowohl nach ICD-10 als auch nach ICD-11 an, eine Einschätzung der Funktionen nach ICF aus Kapitel V ICD-11, eine Einschätzung der Teilhabebeeinträchtigung sowie von Umweltfaktoren in Anlehnung an die ICF und schließlich eine Zusammenfassung nach dem bio-psycho-sozialen Modell. Vor allem durch die Möglichkeit der Einschätzung nach ICD-11 und den starken Bezug zur ICF entsteht ein edukativer Effekt für Fachkräfte. Diese Vorlage soll zudem in der Praxis dazu dienen, die Systemlogiken der Jugendhilfe sowie der Eingliederungshilfe einander anzupassen und die Jugendhilfe auf die „große Lösung“ 2028 vorzubereiten.
Can Serum Thyroglobulin Levels Help to Identify the Involved Neck
Compartment of Differentiated Thyroid Carcinoma?
Authors: Bahçecioğlu Adile Begüm , Ozkan Elgin , Araz Mine , Elhan Atilla Halil , Erdoğan Murat Faik 
Published: September 2022

AbstractWe aimed to evaluate the predictive ability of serum thyroglobulin (Tg) levels on
the localization of the metastatic lymph node compartments in locoregional
metastases of papillary thyroid cancer (PTC). This retrospective study included
143 patients who underwent neck dissections for a total of 172 for
persistent/recurrent locoregional PTC. They were grouped according to
the localization of lymph node metastasis (LNM): Central (C-LNM), Lateral
(L-LNM), both central and lateral LNM (C+L LNM). To confirm that the Tg
cutoff discriminated LNM localizations, the sample was categorized as suppressed
(<0.1 mU/l) or non-suppressed (>0.1 mU/l)
according to TSH and ROC analysis. Mixed-effects models were used to investigate
the effect of LNM localization on Tg levels and to eliminate the confounding
effects of TSH, tumor burden (defined as the number and the largest diameter of
LNM), and RAI. Mean Tg levels were 1.43 μg/l for C-LNM
(n=47), 3.7 μg/l for L-LNM (n=99), and
8.60 μg/l for C+L LNM (n=26).
Independent of TSH, tumor burden and RAI, the mean Tg levels of L-LNM and
C+L LNM groups were not significantly different, while that of C-LNM was
significantly lower than those of L-LNM and C+L LNM. To discriminate
C-LNM from L-LNM and C+L LNM in patients with TSH>0.1
mU/l, the optimal cutoff for Tg was 1.05 μg/l
(sensitivity=74.7%, specificity=70.4%,
PPV=87.7%). L-LNM increases serum Tg levels more than C-LNM in
persistent/recurrent locoregional nodal disease of PTC. Tg above
1.05 μg/l may indicate lateral LNM. Tg may be an
important marker for the localization of LNM in the neck.
LINC02875 Upregulation Contributed to Poor Prognosis for the
Hepatocellular Carcinoma and Progression for the Cancerous Cells
Authors: Liu Yujian , Yang Jingrui , Ke Rui-Sheng , Wu Lupeng , Hong Zaifa , Guo Ping , Feng Liuxing , Li Zhimin 
Published: September 2022

AbstractThe prognostic implications and physiological effect of LINC02875 are unknown in
hepatocellular carcinoma (HCC). We sought to examine the prognostic value of
LINC02875 in HCC and assessed its role in HCC cellular function. LINC02875
expression was evaluated by RT-qPCR in HCC specimens and cell lines. LINC02875
expression was subjected to assess the correlation with clinical parameters by
Chi-squared test and overall survival by Kaplan – Meier curve and Cox
regression analysis. The effects of LINC02875 on the biological characteristics
of HCC cells were studied by MTS and Transwell assay. LINC02875 was
high-expressed in HCC, and this was associated with unfavorable clinical
features and poor prognosis of HCC, especially HBV-related HCC. Knockdown of
LINC02875 inhibited the proliferation, migration, and invasion of HCC cells.
miR-485–5p was a downstream microRNA of LINC02875. LINC02875 affects the
prognosis of HCC patients, especially HBV-related ones. LINC02875 represents a
suitable therapeutic target for HCC.
Treatment of Metastatic Colorectal Cancers in Resource-Constrained Low- and Middle-Income Countries (LMICS) Scenario—Outcomes, Practice Patterns, and Commentary on Treatment Costs
Authors: Ramaswamy Anant , Babu Vasu , Kothari Rushabh , Abhinav Ram , Desouza Ashwin , Ventrapati Pradeep , Kumar Amit , Kapoor Akhil , Das Shasanka , Engineer Reena , Saklani Avanish , Ostwal Vikas 
Published: September 2022

Introduction The overall survival (OS) of metastatic colorectal cancers (mCRCs) in clinical practice and resource-constrained low- and middle-income countries (LMICS) like India is not known.
Materials and Methods Data of patients with mCRC treated between January 2013 and August 2017 were accessed from a prospectively maintained database. Demographics, disease characteristics, chemotherapeutic regimens, use of monoclonal antibodies, and survival outcomes in treated patients were collected and analyzed. Costs of treatment options as off 2017 were also interpreted.
Results The data of 403 patients satisfied prespecified inclusion criteria and were included for analysis. The median age of the cohort was 48 years (range: 17–86) with a predominance of rectal cancers (63.3%), liver alone metastases (47.1%), and resected primary (69.7%). Signet ring histology was present in 82 patients (20.3%). The most commonly used first-line regimen (CT1) was modified capecitabine-oxaliplatin (53.3%). Two hundred and nineteen patients (54.3%) received second-line systemic therapy (CT2). Patients received a median of two lines of therapy (range: 1–6). MoAbs were used by 48 patients (13.4%) with CT1 and 34 patients (15.5%) with CT2. Median OS of the entire cohort was 17.61 months (95% confidence interval: 15.48–19.74), which was within the predicted range, as per investigator hypothesis. The presence of signet ring histology (p<0.001), raised carcinoembryonic antigen at baseline (p=0.017), and the absence of a resected primary (p<0.001) predicted inferior median OS.
Conclusions Survival of patients with mCRC in a resource-constrained LMIC scenario like India is approximately 12 to 15 months lower than published trial data. Limited access to targeted therapy and newer expensive treatment options due to financial constraints may contribute to this disparity.
Once-a-Day Ceftriaxone–Amikacin Combination as Empiric Antibiotic Therapy to Enable Outpatient Management of Febrile Neutropenia in Children—16-Year Experience from a Single Institute
Authors: Kanvinde Shailesh , Mulay Atul , Deshpande Anand , Deshmukh Chetan , Patwardhan Sampada 
Published: September 2022

Background To enable outpatient department (OPD) management of febrile neutropenia (FN), we used once-a-day (OD) ceftriaxone–amikacin (CFT-AMK) as empiric antibiotic therapy. Our experience over 16-year period is presented.Methods This was a retrospective study conducted from January2002 to December2017. Inclusion criteria were <18 years of age, undergoing cancer chemotherapy, and having FN. Exclusion criteria were FN after palliative chemotherapy, bone marrow transplantation, or at diagnosis of malignancy. Empiric CFT-AMK was used in all, except those having respiratory distress, hypotension, altered sensorium, paralytic ileus, or clinical evidence of peritonitis. Admission criteria were age <1 year, acute myeloid leukemia (AML) chemotherapy, poor performance status, need for blood transfusions, convenience, insurance, or persistent fever >48 to 72 hours after CFT-AMK. Outcomes analyzed were response (defervescence within 48–72 hours), OPD management, antibiotic upgrade, and mortality. AML diagnosis, >7 days to absolute neutrophil count >0.5 × 109/L, poor performance status, and malignancy not in remission were considered high-risk FN criteria.Results CFT-AMK was given in 877/952 (92.2%) FN episodes. Seventy-six percent had hematolymphoid malignancies. Response, antibiotic upgrade, and mortality were seen in 85.7 and 65.5% (p < 0.0001), 15 and 45.5% (p < 0.0001), and 0 and 2% (p = 0.003) of low- and high-risk patients, respectively. Treatment was started in OPD in 52%, of which 21.6% required subsequent admission. Of those initially admitted, early discharge (hospital stay < 5 days) was possible in 24.6%. Forty-one percent episodes were managed entirely on OPD. Overall, 80% of low-risk and 42% of high-risk episodes received treatment wholly or partially on OPD.Conclusion Our results show empiric OD CFT-AMK allows OPD management for most of the low-risk and a proportion of high-risk FN following chemotherapy in children, without compromising clinical outcomes.