bluuebunny/crossref_metadata_2025_split huggingface dataset:
Out-of-pocket Expenditure for Antenatal Care Instills amidst Free Health Care Provision: Evidence from a Large Pregnancy Cohort in Rural Sri Lanka
Authors: Gunarathne Sajaan Praveena , Wickramasinghe Nuwan Darshana , Agampodi Thilini Chanchala , Prasanna Indika Ruwan , Agampodi Suneth Buddhika 
Published: August 2022

Abstract
Background: Out-of-pocket expenditure (OOPE) considers a barrier to access to healthcare for pregnant women. This study aimed to estimate the magnitude and associated factors of OOPE for antenatal care (ANC) in a rural Sri Lankan setting. Methods: This study was a follow-up of a large pregnancy cohort [Rajarata Pregnancy Cohort (RaPCo)] in all 22 Medical Officers of Health areas in the Anuradhapura District, Sri Lanka. A pre-tested interviewer-administered questionnaire was used to collect data on socio-demographic, economic, and the cost incurred in the first trimester, and self-administered questionnaires were used monthly to collect costs incurred in the second and third trimesters. In-depth financial information of 1,558 pregnant women was analyzed using descriptive statistics for the magnitude, non-parametric statistical methods for statistically significant difference, and the multiple linear regression model to test the associated factors. Results: The mean (SD)]age of the pregnant women was 28.3 (5.6) years. The mean (SD) monthly household income and expenditure were USD 277.29 (216.04) and USD 190.19 (103.11), respectively. The mean OOPE for per visit ANC was USD 4.18 (52.1% of per day household expenditure). The mean OOPE for total ANC was USD 57.74 (3.4% of annual household expenditure). The direct medical OOPE share (73.8%) was significantly higher than direct non-medical OOPE (26.2%) (p<0.001). Pregnant women who utilized only the government-free health services also spent 28% and 14% of OOPE on medicines and laboratory investigations. Monthly household income (p<0.001), monthly household expenditure (p<0.1), used healthcare mode (p<0.05), having maternal morbidities (p<0.05), and the number of previous pregnancies (p<0.1) were the statistically significant independent predictors of OOPE during ANC.Conclusion: Per visit OOPE for ANC is equivalent to half of the per day household expenditure. Monthly household income, expenditure, used healthcare mode, maternal morbidities, and the number of previous pregnancies are independent predictors of OOPE during ANC. Despite having freely available government health facilities, the majority tend to use private health facilities and incur higher OOPE. Government-free healthcare users also incur a direct medical OOPE for medicines and laboratory investigations.
Development and Evaluation of a Rapid, Specific, and Sensitive Loop-mediated Isothermal Amplification Assay to Detect Tenacibaculum sp. strain Pbs-1 Associated with Black-spot Shell Disease of Akoya Pearl Oysters
Authors: Sakatoku Akihiro , Suzuki Takaya , Tatamiya Yuri , Seki Makoto , Tanaka Daisuke , Nakamura Shogo , Isshiki Tadashi 
Published: August 2022

Abstract
Black-spot shell disease decreases pearl quality and threatens pearl oyster survival. Establishment of a rapid, specific, and sensitive assay to detect Tenacibaculum sp. strain Pbs-1 associated with black-spot shell disease is of commercial importance. We develop a rapid, specific, and highly sensitive loop-mediated isothermal amplification (LAMP) assay to detect Tenacibaculum sp. Pbs-1. A set of five specific primers (two inner, two outer, and a loop) are designed based on the 16S–23S internal spacer region of strain Pbs-1. The optimum reaction temperature, and concentration of inner and loop primers are 63°C, and 1.4 µM and 1.0 µM, respectively. The LAMP product can be detected using agarose gel electrophoresis, and the color change in the reaction tube can be detected visually (by naked eye) following addition of malachite green. Our assay is specific for Pbs-1, with no cross-reactivity with other species in the genus Tenacibaculum. The detection limit of the LAMP assay at 35 min is 50 pg, and at 60 min it is 5 fg. We evaluate the LAMP assay using diseased and healthy pearl oysters. Results indicate the suitability and simplicity of this test for rapid field diagnosis of Pbs-1.
Enhancement of hyphae growth and medium optimization by Pleurotus eryngii-3 under submerged fermentation
Authors: Liu Changqing , Feng Jiajie , Wang Simin , Cao Yi , Shen Yuxiang 
Published: August 2022

Abstract
There were a variety of amino acids and polysaccharides in Pleurotus eryngii contained that could improve human immunity and prevent cancer. In this paper, the medium was screened and carton and nitrogen sources were screened and optimized as 40 g/L corn flour and soybean meal by P. eryngii-3. Then, the effect of pH, temperature, volume, and rotate speed were investigated, in which the suitable fermentation conditions were respectively pH = 6, 27 ℃, 90 mL of fermentation broth, and 150 r/min of rotate speed. Meanwhile, the transcription levels of lcc1, lcc5, lcc8, and lcc12 were up-regulated by 10.25-, 3.32-, 2.33- and 6.29-fold respectively after optimization. In addition, the box-behnken design was utilized to improve DCW (17.23 g/L). This research was optimized the medium and condition of fermentation by P. eryngii-3, which could shorten the ripening cycle of seeds and improve economic benefits for industries.
Porous microneedle patch with sustained exosome delivery repairs severe spinal cord injury
Authors: Fang Ao , Wang Yifan , Guan Naiyu , Lin Lingmin , Guo Binjie , Cai Wanxiong , Chen Xiangfeng , Ye Jingjia , Abdelrahman Zeinab , Li Xiaodan , Zuo Yanming , Zheng Hanyu , Wu Zhonghan , Jin Shuang , Xu Kan , Gu Xiaosong , Yu Bin , Wang Xuhua 
Published: August 2022

Abstract
Mesenchymal stem cell-derived exosome (MSC-EXO) transplantation has been suggested as an efficacious treatment to suppress spinal cord injury (SCI)-triggered neuroinflammation. However, an ethically acceptable method to continuously deliver MSC-EXOs to acute spinal lesions, without damaging nearby tissues/axons, has never been achieved. In this study, we fabricated a device comprising a patch containing MSCs and a microneedle array (MN-MSC patch) to treat severe SCI. When topically applied to an acute spinal lesion beneath the spinal dura, the soft microneedle (MN) array with reasonable mechanical strength avoided damaging the nearby spinal tissues, and the porous microstructure of MNs facilitated highly efficient MSC-EXO delivery. With the capacity for sustained delivery of MSC-EXOs, the MN-MSC patch was evaluated in a contusive rat SCI model. The MSCs encapsulated in the patch could survive for at least 7 days, encompassing the optimal time window for downregulating SCI-triggered neuroinflammation. As a result, MN-MSC patch treatment led to reduced cavity and scar tissue formation, greater angiogenesis, and improved survival of nearby tissues/axons. Remarkably, rats treated by this method achieved superior muscle control and exhibited robust hindlimb locomotion functional recovery. Conclusively, the MN-MSC patch device proposed here overcomes the current dilemma between treatment efficacy and ethical issues in treating acute SCI.
Drug related problems identified by pharmacist in geriatric inpatients in university teaching hospital
Authors: Baralić Ivana , Arsenovic Branka 
Published: August 2022

Abstract
Background
The elderly population is often exposed to complex medication regimens. Increased drug use is one of the main reasons for drug-related problems (DRPs).
Aim
The primary objective of this study was to define and classify DRPs in patients admitted at the Clinical Department of Geriatrics, Zvezdara University Medical Center, Belgrade, Serbia.
Method
Patient demographics, medications, and medical history were evaluated by the pharmacist during a weekly 3-hour visit for a period of 5 months. The DRPs were identified via V9.0 PCNE classification. Lexicomp® was used to assess the drug–drug interactions.
Results
A total of 100 geriatric patients were included in the study (mean age 79.7 ± 7.88 years; median [IQR] of prescribed drugs 8, 5 [6–10]). Total 236 potential DRP were identified and at least one potential DRP was seen in 85% of the patients. The most common underlying DRP concerned incorrect dose (36%), inappropriate drug combination (17.8%), untreated indication (17.4%) and drugs without indication (16.5%). There were significant correlations (p ≤ 0.05) between DRPs and number of comorbidities (r = 0.33) and number of drugs used (r = 0.6). Patients with atrial fibrillation, cardiomyopathy, diabetes mellitus and chronic renal failure had significantly more DRPs in comparison to the patients without these co-morbidities.
Conclusion
Pharmacist was able to detect a high number of DRPs in older patients. The role of the clinical pharmacist might be to improve treatement safety, to adjust the dosage, to detect the over- and underuse of drugs, since these were the most encountered causes of DRPs.
Effect of low-level diode laser and light emitting diode irradiation on cell proliferation and osteogenic/odontogenic differentiation of stem cells from the apical papilla
Authors: Rahmati Afsaneh , Abbasi Roshanak , najafi Rezvan , Rezaei-soufi Loghman , Karkehabadi Hamed 
Published: August 2022

Abstract
Background
This study aimed to assess the effect of irradiation of light-emitting diode (LED) and low-level diode laser on osteogenic/odontogenic differentiation of stem cells from the apical papilla (SCAPs).
Materials and Methods
SCAPs were isolated from the human tooth root. The experimental groups were subjected to 2–4 J/cm2 diode laser and LED irradiation in osteogenic medium. The control group did not receive any irradiation. Cell proliferation was assessed by the methyl thiazolyl tetrazolium (MTT) assay. Osteogenic differentiation was evaluated by alizarin red staining (ARS), and expression of osteogenic genes by real-time polymerase chain reaction (RT-PCR).
Results
The MTT assay showed no significant difference in cell viability/proliferation of SCAPs in the laser, LED, and control groups at 24 or 48 hours (P < 0.001). The ARS assessment showed that diode laser and red LED irradiation caused osteogenic differentiation of SCAPs. Diode laser and red LED irradiation both caused over-expression of osteogenic/dentinogenic genes including alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1), and bone sialoprotein (BSP) in SCAPs. Up-regulation of genes was significantly greater in diode laser irradiation group than LED group (P < 0.001).
Conclusions
Diode laser with 2–4 J/cm2 energy density and LED can cause osteogenic differentiation and proliferation of SCAPs. Further studies are required to assess the mechanism of effect of diode laser and LED.
Numerical simulation of heat pump drying system for paper exhaust hood
Authors: Yan Yan , Li WEN 
Published: August 2022

Abstract
In view of the present situation that the exhaust waste heat of the hot air hood in the paper drying section is too much and the environment is not friendly, a closed heat pump drying system model applied to the exhaust air hood is proposed and established. Effects of evaporation temperature and condensation temperature on supply air temperature, supply air moisture content, system performance coefficient (COP) and dehumidification energy consumption ratio (SMER) were studied. Results show that with the increase of condensation temperature, the supply air temperature is basically unchanged, and the moisture content of the supply air increases, while the COP and SMER of the system show a downward trend. The moisture content of supply air decreases with the increase of evaporation temperature, while the supply air temperature, COP and SMER all show an upward trend. Therefore, in order to improve the drying characteristics and improve the performance of the heat pump system, the condensation temperature can be reduced and the evaporation temperature can be increased.
Preparation of hierarchical porous carbon through one-step KOH activation of coconut shell biomass for high-performance supercapacitor
Authors: Zhao Yutong , Wang Yuanyuan , Liu Yanxiu , Wang Huan , Song Hua 
Published: August 2022

Abstract
Here we report an effective and facile method for preparing porous carbons (CSCK-T-x) with highly developed hierarchical porosity for high-performance supercapacitor by one-step KOH activation of coconut shell carbon. The effects of carbonization temperature (T, oC) and KOH/C ratio (x) on the structure and electrochemical properties were studied systematically. The as-prepared CSCK-800-2 displayed two concentrated mesopores at 4 nm and 14 nm. The unique hierarchical structure of CSCK-800-2 enables it to have low ion transport resistance and high ion-accessible surface area, which contributes to outstanding electrochemical performance. Furthermore, it possessed the highest BET specific surface area of 2143.6 m2·g− 1 with a pore volume of 1.34 cm3·g− 1 among the CSCK-T-x. In a three-electrode test system, CSCK-800-2 exhibits a high specific capacitance of 317 F·g− 1 at a current density of 0.5 A·g− 1 and considerable rate retention of 68% at 20 A·g− 1. The symmetrical supercapacitor that was based on CSCK-800-2 showed a maximum energy density of 13.5 Wh·kg− 1 at 0.5 A·g− 1 in 6M KOH electrolyte. Besides, it also exhibited a superior cycling stability with 99.7% of the capacitance retention after 10000 cycles at 5 A·g− 1.
BRCA1 and BRCA2 gene mutation spectrum and a high frequency of BRCA1 185delAG among breast and ovarian cancer patients from Southern India.
Authors: John Ajoy Oommen , Singh Ashish , Joel Anjana , Yadav Pratibha , Thumaty Divya Bala , K Fibi Ninan , Georgy Josh Thomas , Cherian Anish Jacob , Thomas Shawn , Thomas Anitha , Thomas Vinotha , Peedicayil Abraham , Abraham Deepak , Thomas Nihal , Varghese Deny , R Parthiban , Ravichandran Lavanya , Johnson Jabasteen , Yadav Bijesh , S Patricia , B Selvamani , Paul MJ , Chacko Raju Titus , Chapla Aaron 
Published: August 2022

Abstract
Purpose
In a developing country like India, genomic data sets for even the most clinically relevant genes like BRCA1 and BRCA2 is rather scarce. Also, there is a need to identify and screen population specific BRCA hotspots to pave a way for affordable genetic testing strategies in clinical practice.
Method
This is an ambispective study to evaluate an NGS based approach to identify pathogenic variants in BRCA1 and BRCA2 genes among 430 patients with Breast and Ovarian cancers. The target enrichment was carried out using the in-house designed Multiplex-PCR followed by targeted Next-generation sequencing (NGS) for BRCA1 and BRCA2. Also, allele-specific PCR based genotyping of del185AG was carried out in additional 120 patients.
Results
In this study, we have identified 100 BRCA1 and BRCA2 variants and based on ACMG 2015 guidelines, these variants were classified as 46 pathogenic, 9 likely pathogenic, and 45 VUS. Of these variants, three were novel, two with likely pathogenic, and one variant of uncertain significance (VUS). The 185delAG was identified as a recurrent mutation in the Southern Indian population accounting for 25.45% of the pathogenic variants. In addition, a family history of cancers of the breast, ovary, pancreas, or prostate (BOPP) was found to be associated with a higher risk of identifying a deleterious BRCA1/2 variant [OR 3.2 (95%CI 1.84–5.77) p ≤ 0.001].
Conclusions
These results suggest that Multiplex PCR-NGS is a sensitive and specific strategy for BRCA testing. However, ASPCR-based genotyping of BRCA1(NM_007300.4): c.68_69del followed by targeted NGS would be a cost-effective approach in south Indian patients.
Prevalence of the porcine cytomegalovirus virus (PCMV), a porcine roseolovirus, in wild boars in Italy and Germany
Authors: Hansen Sabrina , Franzo Giovanni , Menandro Maria Luisa , Krabben Ludwig , Marino Stephen Francis , Kaufer Benedikt , Denner Joachim 
Published: August 2022

Abstract
Porcine cytomegalovirus (PCMV), which is a porcine roseolovirus (PRV), closely related to the human herpesviruses 6 and 7, is common among commercial pigs. PCMV/PRV was shown to be important in xenotransplantation because transmission of PCMV/PRV in preclinical trials transplanting pig organs into non-human primates was associated with a significantly reduced survival of the xenotransplants. PCMV/PRV was also transmitted in the worldwide first pig heart transplantation into a human patient and obviously contributed to the death of the patient. The prevalence of PCMV/PRV in wild boars is largely unknown. Here, we screen wild boars from several places in Northern Italy and Germany to evaluate the prevalence of PCMV/PRV using PCR-based and Western blot assays. By Western blot analysis 65% and 82% of the Italian and German wild boars were found to be PCMV/PRV-positive, while 36% and 60% tested positive by real-time PCR, respectively. These data indicate that the prevalence in German wild boars is higher compared with the Italian and that the Western blot assay is a more robust tool to detect a PCMV/PRV infection. This data also indicates that pigs generated for xenotransplantation should be secured from contact with materials from wild boars besides commercial pigs .
Extended Spectrum Betalactamase Research mapping; a 32-year Temporal and geographical perspective.
Authors: Suleiman Adeiza Shuaibu , Abdulmalik Shuaibu Bello , Ghazali Shuaibu Muhammad 
Published: August 2022

Abstract
Background
β-lactam inactivating enzymes like Extended Spectrum β-lactamases (ESBL) have been the focus of the scientific community for decades. The current study assesses the scientific focus on ESBLs based on the temporal succession of ESBL producing pathogens, ESBL types, the blaESBL gene, antibacterial agents, co-existence of resistance determinants, and clonal lineages over a 32-year period.
Method
Bibliometric indices of publications between 1990 and 2022 were assessed. Network mapping, visualizations and text mining were rendered with Bibliometrix package in R- studio and Visualization of Similarities (VOS) Viewer software.
Results
A total of 8890 documents were retrieved, with 6246 (70.3 percent) articles in the Medical and Health Sciences category. Despite being the most productive country, with 956 (10.8%) articles published, none of the top 20 institutions were from the United States. Four Spanish and three French institutions made the top ten. Despite the fact that the number and type of antimicrobial agents indicated against ESBL producing organisms changed over time, the preference for Piperacillin/Tazobactam remained consistent for decades. Pseudomonas aeruginosa, Klebsiella pneumonia, and Escherichia coli were the most frequently encountered pathogens of clinical and veterinary importance. E. coli clones (ST(s) 131, 117, 69, 73, 95, 410, 648, 10 and ST38) and K. pneumoniae clones (ST(s) 101, 15, 307, 147, 11, 405, 16 and 23) were the most frequently reported. Previous research also highlighted 'Emerging therapy' based on a 'new ESBL gene' and 'globalization of CTX-M'. Recent top-cited studies focused on 'hypervirulence' and MDR in invasive Klebsiella sp ', 'newer β-lactam/β-lactamase inhibitor (BLBLI) combinations,' and a 'new ultra-broad-spectrum' β-lactamase inhibitor of serine metallo-β-lactamases (QPX7728). Another notable subtheme has been the spread of ESBL and 'Carbapenemase Encoding Genes' in the 'natural aquatic environment'.
Conclusion
ESBLs are highly researched members of the beta-lactamase family with over 256,980 citations and counting. The scientific community is focused on the mitigation of plasmid mediated spread of ESBLs especially blaCTX−M gene. Reducing mortality by the development of a clinical cure for ESBL infections is a major trend in human and veterinary medicine. Workable multi-country research collaborations are compulsory to consolidate regional guidelines for AMR into a global one.
Anomalous Citations Detection in Academic Networks
Authors: Liu Jiaying , Bai Xiaomei , Tuarob Suppawong , Xia Feng 
Published: August 2022

Abstract
Citation network analysis attracts increasing attention from disciplines of complex network analysis and science of science. One big challenge in this regard is that there are unreasonable citations in citation networks, i.e., cited papers are not relevant to the citing paper. Existing research on citation analysis has primarily concentrated on the contents and ignored the complex relations between academic entities. In this paper, we propose a novel research topic, that is, how to detect anomalous citations. To be specific, we first define anomalous citations and propose a unified framework, named ACTION, to detect anomalous citations in a heterogeneous academic network. ACTION is established based on non-negative matrix factorization and network representation learning, which considers not only the relevance of citation contents but also the relationships among academic entities including journals, papers, and authors. To evaluate the performance of ACTION, we construct two anomalous citation datasets. Experimental results demonstrate the effectiveness of the proposed method. Detecting anomalous citations carry profound significance for academic fairness.
Deep Learning-Based Idiomatic Expression Recognition for the Amharic Language
Authors: Endalie Demeke , Haile Getamesay , taye wondmagegn 
Published: August 2022

Abstract
Idioms are used in Amharic to conceal information or to express ideas indirectly. However, most natural language processing models used with the Amharic language, such as machine translation, semantic analysis, sentiment analysis, information retrieval, question answering, and next word prediction, do not consider idiomatic expressions. As a result, in this paper, we proposed a conventional neural network (CNN) with a FastText embedding model for detecting idioms in an Amharic text. We collected 1700 idiomatic and 1600 non-idiomatic clause datasets from Amharic books to test the proposed model's performance. The proposed model is then evaluated using this dataset. With testing and training datasets, the proposed model achieves an accuracy of 80% and 98%, respectively. We compared the proposed model to other machine learning models like K-Nearest Neighbor (KNN), Support Vector Machine (SVM), and Random Forest classifiers. According to the experimental results, the proposed model produces promising results.