bluuebunny/crossref_metadata_2025_split huggingface dataset:
Timing and evolution of PSR B0950+08
Authors: Huang Hai-tao , Zhou Xia , Yuan Jian-ping , Zheng Xiao-Ping 
Published: March 2022

ABSTRACT
We present timing solutions of PSR B0950+08, using 14 years of observations from the Nanshan 26-m Radio Telescope of Xinjiang Astronomical Observatory. The braking index of PSR B0950+08 varies from –367 392 to 168 883, which shows an oscillation with large amplitude (∼105) and uncertainty. Considering the variation of braking indices and the most probable kinematic age of PSR B0950+08, a model with long-term magnetic field decay modulated by short-term oscillations is proposed to explain the timing data. With this magnetic field decay model, we discuss the spin and thermal evolution of PSR B0950+08. The uncertainties of its age are also considered. The results show that three-component oscillations are the more reasonable for the spin-frequency derivative distributions of PSR B0950+08, and the initial spin period of PSR B0950+08 must be shorter than $97\rm \ ms$ when the age is equal to the lower bound of its kinematic age. The standard cooling model could explain the surface temperature of PSR B0950+08 with its most probable kinematic age. Vortex creep heating with a long-term magnetic field decay could maintain a relatively high temperature at the later stages of evolution and explain the thermal emission data of old and warm pulsars. Coupling with the long-term magnetic field decay, an explanation of the temperature of PSR B0950+08 with roto-chemical heating needs an implausibly short initial rotation period ($P_0 \lesssim 17\rm { ms}$). The spin and thermal evolution of pulsars should be studied simultaneously. Future timing, ultraviolet or X-ray observations are essential for studying the evolution and interior properties of pulsars.
Follow the water: finding water, snow, and clouds on terrestrial exoplanets with photometry and machine learning
Authors: Pham Dang , Kaltenegger Lisa 
Published: March 2022

ABSTRACT
All life on Earth needs water. NASA’s quest to follow the water links water to the search for life in the cosmos. Telescopes like the James Webb Space Telescope and mission concepts like HabEx, LUVOIR, and Origins are designed to characterize rocky exoplanets spectroscopically. However, spectroscopy remains time-intensive, and therefore, initial characterization is critical to prioritization of targets. Here, we study machine learning as a tool to assess water’s existence through broad-band filter reflected photometric flux on Earth-like exoplanets in three forms: seawater, water-clouds, and snow; based on 53 130 spectra of cold, Earth-like planets with six major surfaces. XGBoost, a well-known machine-learning algorithm, achieves over 90 per cent balanced accuracy in detecting the existence of snow or clouds for S/N ≳ 20, and 70 per cent for liquid seawater for S/N ≳ 30. Finally, we perform mock Bayesian analysis with Markov chain Monte Carlo with five filters identified to derive exact surface compositions to test for retrieval feasibility. The results show that the use of machine learning to identify water on the surface of exoplanets from broad-band filter photometry provides a promising initial characterization tool of water in different forms. Planned small and large telescope missions could use this to aid their prioritization of targets for time-intense follow-up observations.
Geography-Dependent Horizontal Gene Transfer from Vertebrate Predators to Their Prey
Authors: Kambayashi Chiaki , Kakehashi Ryosuke , Sato Yusuke , Mizuno Hideaki , Tanabe Hideyuki , Rakotoarison Andolalao , Künzel Sven , Furuno Nobuaki , Ohshima Kazuhiko , Kumazawa Yoshinori , Nagy Zoltán T. , Mori Akira , Allison Allen , Donnellan Stephen C. , Ota Hidetoshi , Hoso Masaki , Yanagida Tetsuya , Sato Hiroshi , Vences Miguel , Kurabayashi Atsushi 
Published: March 2022

AbstractHorizontal transfer (HT) of genes between multicellular animals, once thought to be extremely rare, is being more commonly detected, but its global geographic trend and transfer mechanism have not been investigated. We discovered a unique HT pattern of Bovine-B (BovB) LINE retrotransposons in vertebrates, with a bizarre transfer direction from predators (snakes) to their prey (frogs). At least 54 instances of BovB HT were detected, which we estimate to have occurred across time between 85 and 1.3 Ma. Using comprehensive transcontinental sampling, our study demonstrates that BovB HT is highly prevalent in one geographical region, Madagascar, suggesting important regional differences in the occurrence of HTs. We discovered parasite vectors that may plausibly transmit BovB and found that the proportion of BovB-positive parasites is also high in Madagascar where BovB thus might be physically transported by parasites to diverse vertebrates, potentially including humans. Remarkably, in two frog lineages, BovB HT occurred after migration from a non-HT area (Africa) to the HT hotspot (Madagascar). These results provide a novel perspective on how the prevalence of parasites influences the occurrence of HT in a region, similar to pathogens and their vectors in some endemic diseases.
Baseline distribution of stable copper isotope compositions of the brain and other organs in mice
Authors: Moynier Frédéric , Merland Ariane , Rigoussen Dimitri , Moureau Julien , Paquet Marine , Mahan Brandon , Le Borgne Marie 
Published: March 2022

AbstractCopper (Cu) stable isotopes are useful for understanding pathways and tracing changes in Cu homeostasis, such as those induced by various diseases (e.g. liver cirrhosis, numerous forms of cancer, and neurodegenerative diseases). However, this utility relies on a baseline understanding of the natural distribution of Cu isotopes between organs of healthy organisms, which is not well-known at present. Here, the distribution of natural Cu isotopes in the brain, liver, red blood cells, plasma, kidneys, and muscle of 14 mice (7 males and 7 females) from three different genetic backgrounds is assessed. We show that the Cu isotopic composition of most mouse organs is isotopically distinct from one another. The most striking feature is the heavy isotope enrichment of the kidney (δ65Cu = 1.65 ± 0.06‰, 2SE), brain (δ65Cu = 0.87 ± 0.03‰, 2SE) and liver (δ65Cu = 0.71 ± 0.24‰, 2SE) compared to blood components, i.e. red blood cells (RBCs) (δ65Cu = 0.30 ± 0.06‰, 2SE), and plasma (δ65Cu = –0.61 ± 0.08‰, 2SE), with δ65Cu being the per mil deviation of the 65Cu/63Cu ratio from the NIST SRM 976 standard. Differences in genetic background do not appear to affect the isotopic distribution of Cu. Interestingly, male and female mice appear to have different Cu concentrations and isotopic compositions in their brain, plasma, muscle, and RBC.By demonstrating that organs have distinct isotopic compositions, our study reinforces the notion that Cu stable isotopes can be used to trace changes in homeostasis in diseases affecting Cu distribution, such as Alzheimer's disease, liver cancer, and possible chronic kidney failure.
Tamarisk biocontrol alters bird community composition in the absence of cottonwood and willow vegetation
Authors: Mahoney Sean M , Johnson Matthew J , Holmes Jennifer A , Dudley Tom L , Kuehn Michael J , Theimer Tad C 
Published: March 2022

AbstractInvasive plants threaten biodiversity worldwide, but control of non-native species may affect native species in complex ways. Non-native tamarisk (Tamarix spp.) is widespread in western U.S. riparian environments, and tamarisk beetles (Diorhabda spp.), a tamarisk-specific herbivore, were subsequently introduced as biocontrol. The primary effect of biocontrol is defoliation and branch dieback, with repeated defoliation killing the plant. We investigated the initial stages of site recovery after biocontrol and how tamarisk decline affected birds, their arthropod prey, and microclimate. We compared avian community diversity and composition, arthropod abundance, humidity, and temperature at sites along the Virgin River in Nevada and Arizona, USA characterized as either mixed native vegetation, tamarisk-dominated, or tamarisk-dominated where the majority of tamarisk died from biocontrol. We compared avian communities sampled after biocontrol to counts at the same locations before biocontrol. Prior to biocontrol, community compositions of all but one site grouped together using unbiased clustering algorithms. Following biocontrol, tamarisk-dominated sites grouped separately, and mixed sites grouped with the pre-biocontrol cluster. Comparison of pre- and post-biocontrol communities showed 7 common species declined by ≥30% in dead tamarisk sites, while one species did so at mixed sites and 3 at tamarisk-dominated sites. Individual census points in dead tamarisk had significantly lower Simpson diversity than the same points censused before biocontrol, unless native vegetation was present, suggesting tamarisk death was the cause of dominant species abundance changes. Tamarisk-dominated sites were hotter and drier than sites with native vegetation and supported fewer non-tamarisk-obligate arthropods, consistent with the hypothesis that bird reductions were driven by changes in microclimate and prey abundance. How long these effects last will depend upon the rate of native vegetation recovery after biocontrol, therefore we recommend monitoring sites to determine the trajectory of vegetative recovery and considering the need and feasibility of active restoration in those sites with slow or no native regeneration.
Pleiotropic and nonredundant effects of an auxin importer in Setaria and maize
Authors: Zhu Chuanmei , Box Mathew S , Thiruppathi Dhineshkumar , Hu Hao , Yu Yunqing , Martin Callista , Doust Andrew N , McSteen Paula , Kellogg Elizabeth A 
Published: March 2022

Abstract
Directional transport of auxin is critical for inflorescence and floral development in flowering plants, but the role of auxin influx carriers (AUX1 proteins) has been largely overlooked. Taking advantage of available AUX1 mutants in green millet (Setaria viridis) and maize (Zea mays), we uncover previously unreported aspects of plant development that are affected by auxin influx, including higher order branches in the inflorescence, stigma branch number, glume (floral bract) development, and plant fertility. However, disruption of auxin flux does not affect all parts of the plant, with little obvious effect on inflorescence meristem size, time to flowering, and anther morphology. In double mutant studies in maize, disruptions of ZmAUX1 also affect vegetative development. A green fluorescent protein (GFP)-tagged construct of the Setaria AUX1 protein Sparse Panicle1 (SPP1) under its native promoter showed that SPP1 localizes to the plasma membrane of outer tissue layers in both roots and inflorescences, and accumulates specifically in inflorescence branch meristems, consistent with the mutant phenotype and expected auxin maxima. RNA-seq analysis indicated that most gene expression modules are conserved between mutant and wild-type plants, with only a few hundred genes differentially expressed in spp1 inflorescences. Using clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 technology, we disrupted SPP1 and the other four AUX1 homologs in S. viridis. SPP1 has a larger effect on inflorescence development than the others, although all contribute to plant height, tiller formation, and leaf and root development. The AUX1 importers are thus not fully redundant in S. viridis. Our detailed phenotypic characterization plus a stable GFP-tagged line offer tools for future dissection of the function of auxin influx proteins.
Nailfold microvascular abnormalities are associated with a higher prevalence of pulmonary arterial hypertension in patients with MCTD
Authors: Todoroki Yasuyuki , Kubo Satoshi , Nakano Kazuhisa , Miyazaki Yusuke , Ueno Masanobu , Satoh-Kanda Yurie , Kanda Ryuichiro , Miyagawa Ippei , Hanami Kentaro , Nakatsuka Keisuke , Saito Kazuyoshi , Nakayamada Shingo , Tanaka Yoshiya 
Published: March 2022

Abstract

Objective
MCTD manifests with microvasculopathy and overlapping clinical features of SLE, SSc and idiopathic inflammatory myopathies (IIM). The aim of this study was to investigate the clinical significance of microvasculopathy in patients with MCTD using nailfold videocapillaroscopy (NVC).

Methods
Fifty patients with newly diagnosed and untreated MCTD were enrolled in this multicentre, prospective and observational study. Clinical features and NVC findings were assessed at baseline and after 1 year post-intervention, along with disease controls [SLE (n = 40), SSc (n = 70) and IIM (n = 50)].

Results
All MCTD patients presented Raynaud’s phenomenon and were positive for anti-U1 RNP antibodies, and 22.0% (11/50) had pulmonary arterial hypertension (PAH). The prevalence of NVC scleroderma patterns in MCTD was 38.0%, which was lower than SSc (88.6%) but higher than SLE (10.0%). In addition, when we divided MCTD patients into two groups by presence or absence of NVC scleroderma patterns, we found a higher prevalence of PAH in patients with NVC scleroderma patterns. Namely, NVC scleroderma patterns were observed in all MCTD patients with PAH, and in 21.0% of those without PAH. After intensive immunosuppressive therapy, NVC scleroderma patterns disappeared in half of the MCTD patients but were not changed in SSc patients.

Conclusions
MCTD differed from SLE, SSc and IIM in terms of the prevalence and responsiveness of NVC scleroderma patterns to immunosuppressive therapy. Detection of nailfold microvascular abnormalities in MCTD could contribute to predicting PAH and help us to understand further aspects of the pathogenesis of MCTD.
Conceptual limits of performativity: assessing the feasibility of market design blueprints
Authors: Rilinger Georg 
Published: March 2022

Abstract
Market designers work as social engineers. They create institutional structures to enforce the assumptions of theoretical market mechanisms. Despite much work on performative effects of economics, sociology has not analyzed the feasibility of designers’ theoretical blueprints. This article suggests that this may be done by identifying assumptions that derail the mechanism if they are not enforced. Sociologists then need to trace obstacles to their realization in practices of implementation. Blueprints are infeasible if the assumptions cannot be enforced either by themselves or jointly. To illustrate the approach, the article identifies one such assumption and traces its impact on a historical experiment of market design: the creation of markets for transmission capacity in California’s electricity markets. To realize the design’s explicit assumptions, designers built institutions that violated an implicit uniformity assumption and created opportunities for manipulations. Since the design’s assumptions could not be realized simultaneously, it was infeasible.
Bringing the market in: an expanded framework for understanding popular responses to economic inequality
Authors: Lindh Arvid , McCall Leslie 
Published: March 2022

Abstract
There has been an upsurge in research on the politics of economic inequality in the 21st century. Discussions of popular responses to rising inequality have expanded in turn yet remain centered on support for explicit government redistribution of income from the rich to the poor. We argue that this focus may downwardly bias measurement of preferences for less inequality and propose an expanded framework that incorporates preferences regarding market institutions and policies that reduce labor market inequalities, which together can shed light on the public’s adherence to free market ideology. Empirically, we use original data to examine the US case, with a comparison to Sweden and Denmark. The results offer initial evidence of (1) the need for an expanded framework and research agenda in this field and (2) potentially broad-based support for policies that address economic inequality directly in the market sphere, contrary to key tenets of free market ideology.
In search of a laundry receipt
Authors: Au-Yeung Bennett , Leung Samuel Yee Ching 
Published: March 2022

Abstract
There are, in general, three widely considered theoretical bases for a knowing receipt claim: unconscionability, a subsisting proprietary interest and unjust enrichment. In Byers v Saudi National Bank, the English Court of Appeal seemed to have settled for the first two bases. In this article, we first question why unconscionability alone cannot serve as the basis for a knowing receipt claim. We also bring to light the practical consequence that the Court’s decision may have in encouraging unscrupulous third parties to launder clean assets obtained in breach of trust by transferring them to safe-haven jurisdictions where the trust regime is not recognised.
The impact of Loa loa microfilaraemia on research subject retention during a whole sporozoite malaria vaccine trial in Equatorial Guinea
Authors: Manock Stephen R , Nsue Vicente Urbano , Olotu Ally , Mpina Maximillian , Nyakarungu Elizabeth , Raso José , Mtoro Ali , Ondo Mangue Martín Eka , Ntutumu Pasialo Beltrán Ekua , Nguema Rufino , Riyahi Pouria , Schindler Tobias , Daubenberger Claudia , Church L W Preston , Billingsley Peter F , Richie Thomas L , Abdulla Salim , Hoffman Stephen L 
Published: March 2022

Abstract
Loa loa microfilariae were found on thick blood smears (TBSs) from 8 of 300 (2.7%) residents of Bioko Island, Equatorial Guinea, during a Plasmodium falciparum sporozoite malaria vaccine clinical trial. Only one subject was found to have microfilaraemia on his first exam; parasites were not discovered in the other seven until subsequent TBSs were performed, at times many weeks into the study. All infected individuals were asymptomatic, and were offered treatment with diethylcarbamazine, per national guidelines. L. loa microfilaraemia complicated the enrolment or continued participation of these eight trial subjects, and only one was able to complete all study procedures. If ruling out loiasis is deemed to be important during clinical trials, tests that are more sensitive than TBSs should be performed.
Cryptic lineages, cryptic barriers: historical seascapes and oceanic fronts drive genetic diversity in supralittoral rockpool beetles (Coleoptera: Hydraenidae)
Authors: Villastrigo Adrián , Bilton David T , Abellán Pedro , Millán Andrés , Ribera Ignacio , Velasco Josefa 
Published: March 2022

Abstract
Morphologically cryptic lineages confound many estimates of global biodiversity and are often discovered in ecologically specialized taxa, subject to strong morphological constraint. Such a situation may apply in many extreme environments, including supralittoral rockpools, where dramatic fluctuations in water availability and salinity impose strong selection pressures on the inhabitants. Here we explore the genetic diversity and phylogeography of supralittoral rockpool Ochthebius beetles in the eastern Atlantic and western Mediterranean, using a combination of mitochondrial and nuclear markers and dense geographical sampling of the three recognized widespread species. Our results point to the existence of morphologically cryptic lineages within all currently named taxa and suggest that the distribution of these is linked to both historical and contemporary marine hydrogeography; a combination of ocean currents and winds apparently driving the spatial patterns observed. The main contemporary barrier to dispersal for Ochthebius is located around the Ibiza Channel, whilst the Messinian Salinity Crisis appears to have been the ultimate driver of lineage diversification in these insects. Our results show that oceanographic processes do not just shape the evolution of fully marine species, but also impact significantly on the terrestrially derived inhabitants of the coastal zone.
Sensitivity of Colletotrichum acutatum Species Complex from Strawberry to Fungicide Alternatives to Quinone-Outside Inhibitors
Authors: Rebello Carolina S. , Baggio Juliana S. , Forcelini Bruna B. , Peres Natalia A. 
Published: March 2022

Colletotrichum acutatum is a species complex that causes anthracnose fruit rot and root necrosis on strawberry. The major and minor species within the complex that affect strawberry production are C. nymphaeae and C. fioriniae, respectively. The disease can significantly reduce yield under conducive weather, and its management has greatly relied on quinone-outside inhibitor fungicides (QoI). However, due to the emergence of resistant isolates, such products are no longer effective. Therefore, alternative fungicides were investigated. C. nymphaeae and C. fioriniae isolates were collected from multiple strawberry fields in the United States from 1995 to 2017. The sensitivity of benzovindiflupyr, penthiopyrad, pydiflumetofen, fluazinam, fludioxonil, and cyprodinil was assessed by in vitro and in vivo assays. Both Colletotrichum species were sensitive to benzovindiflupyr, penthiopyrad, fluazinam, and fludioxonil based on mycelial growth assays. Interestingly, of these products, only penthiopyrad did not inhibit conidial germination at 100 µg/ml. For cyprodinil, C. nymphaeae was sensitive based on the mycelial growth, whereas C. fioriniae was not inhibited. Neither species was inhibited by pydiflumetofen in mycelial growth, conidial germination, nor detached fruit assays. The prepackaged mixtures fludioxonil + cyprodinil and fludioxonil + pydiflumetofen were effective in a field trial; however, their use should be carefully considered because of the lack of efficacy of one of the compounds in the mixture. This study sheds light on the potential registration of products alternative to QoIs, such as benzovindiflupyr and fluazinam, which could improve the management of strawberry anthracnose.
Mechanisms of GZ17-6.02 resistance
Authors: Booth Laurence , West Cameron , Von Hoff Daniel , Dent Paul 
Published: March 2022

Objectives
The drug GZ17-6.02 is undergoing phase I in solid tumor patients (NCT03775525). The present studies initially determined the impact of prolonged exposure of colorectal tumors to GZ17-6.02, and to determine whether GZ17-6.02 enhanced the efficacy of an anti-PD1 antibody. Subsequently, studies defined the evolutionary resistance mechanisms in tumor cells previously exposed to GZ17-6.02.

Methods
IACUC-approved animal studies were performed. In cell immunoblotting, cell transfections and trypan blue death assays were performed.

Results
Prolonged exposure of colorectal tumors to GZ17-6.02 enhanced the efficacy of 5-fluorouracil and of an anti-PD1 antibody, significantly prolonging animal survival. Tumor cells previously exposed to GZ17-6.02 in vivo had elevated their expression of ERBB2 and ERBB3, and increased phosphorylation of ERBB1, ERBB3, PDGFRβ, AKT T308, ERK1/2, p70 S6K T389, STAT5 Y694 and c-SRC Y416. The phosphorylation of c-SRC Y527 declined. The efficacy of ERBB receptor inhibitors at killing these resistant tumor cells was unaltered by prior GZ17-6.02 exposure whereas the efficacy of multi-kinase/PDGFRβ inhibitors was significantly reduced. Treatment of colon cancer cells with GZ17-6.02 rapidly reduced the levels of multiple HDAC proteins and altered their subcellular localization. Isolates from resistant tumors expressed less CD95 and FAS-L. HDAC inhibitors enhanced CD95 and FAS-L levels in the resistant cells via activation of NFκB and HDAC inhibitors restored the efficacy of GZ17-6.02 to near control levels.

Conclusions
Our findings demonstrate that GZ17-6.02 has the potential to be developed as a colon cancer therapeutic and that resistance to the drug can be partially reversed by HDAC inhibitors.