bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural Contributions to Reduced Fluid Intelligence across the Adult Lifespan
Authors: Mitchell Daniel J. , Mousley Alexa L. S. , Shafto Meredith A. ,   Cam-CAN, Duncan John 
Published: December 2022

Fluid intelligence, the ability to solve novel, complex problems, declines steeply during healthy human aging. Using fMRI, fluid intelligence has been repeatedly associated with activation of a frontoparietal brain network, and impairment following focal damage to these regions suggests that fluid intelligence depends on their integrity. It is therefore possible that age-related functional differences in frontoparietal activity contribute to the reduction in fluid intelligence. This paper reports on analysis of the Cambridge Center for Ageing and Neuroscience data, a large, population-based cohort of healthy males and females across the adult lifespan. The data support a model in which age-related differences in fluid intelligence are partially mediated by the responsiveness of frontoparietal regions to novel problem-solving. We first replicate a prior finding of such mediation using an independent sample. We then precisely localize the mediating brain regions, and show that mediation is specifically associated with voxels most activated by cognitive demand, but not with voxels suppressed by cognitive demand. We quantify the robustness of this result to potential unmodeled confounders, and estimate the causal direction of the effects. Finally, exploratory analyses suggest that neural mediation of age-related differences in fluid intelligence is moderated by the variety of regular physical activities, more reliably than by their frequency or duration. An additional moderating role of the variety of nonphysical activities emerged when controlling for head motion. A better understanding of the mechanisms that link healthy aging with lower fluid intelligence may suggest strategies for mitigating such decline.SIGNIFICANCE STATEMENTGlobal populations are living longer, driving urgency to understand age-related cognitive declines. Fluid intelligence is of prime importance because it reflects performance across many domains, and declines especially steeply during healthy aging. Despite consensus that fluid intelligence is associated with particular frontoparietal brain regions, little research has investigated suggestions that under-responsiveness of these regions mediates age-related decline. We replicate a recent demonstration of such mediation, showing specific association with brain regions most activated by cognitive demand, and robustness to moderate confounding by unmodeled variables. By showing that this mediation model is moderated by the variety of regular physical activities, more reliably than by their frequency or duration, we identify a potential modifiable lifestyle factor that may help promote successful aging.
improvement of cardiorespiratory fitness in healthy women after a 12-week Classical Pilates training
Authors: Piccoli Francine , Fischer Gabriela , Brod Marta da Silva , Vargas Carolina Boeira , Vinholes Daniele Botelho , Ribeiro Paula Aver Bretanha , Cardoso Thiago de Araujo , Baptista Rafael Reimann 
Published: December 2022

Aims: to evaluate the effects of 12 weeks of Pilates training (Classical Method) on cardiorespiratory fitness and heart rate responses of healthy sedentary women. Method: fifteen women (average age 29±4) performed 12 weeks of Pilates training for 60 minutes, three times per week (Pilates Group). Thirteen women (average age 29±5) as controls maintained their routine activities (Control Group). The exercises’ introduction was by the degree of difficulty with changes in the plane of movements; smaller base of support; spinal rotations and extensions; use of abdominal muscles in prone position. Heart rate was monitored and recorded during all sessions using a heart frequency meter. Results: ANOVA analysis results revealed significant difference in Pilates Group (p < 0.05) between pre and post measures of VO2peak (+13%), fat percentage (- 3.3%), free fat mass (+ 2.8 kg), and muscular endurance in the abdominal area (+61%), lower limbs (+75%) and upper limbs (+68%). The percent of Maximum Heart Rate achieved in the 6th (79.25%) and 12th (79.86%) weeks of training increased from the first week (73.4%). Physical fitness in Control Group remained unchanged. Conclusion: twelve weeks of Pilates training positively affected overall physical fitness in previously sedentary healthy women. Furthermore, the increase in VO2peak and achieved Maximum Heart Rate was significant even with no specific aerobic training.
Differences in Total S-RBD Antibody SARS-CoV-2 Post-vaccination mRNA-1273 3rd Dosage (Heterologous Prime Booster) with Post-prime Vaccination Inactivated Whole Virus Two Doses (Homologous) in Health Personnel Surabaya H2LC Clinic
Authors: Hartanto Deddy , Nugraha Jusak A. , Budhy Theresia Indah 
Published: December 2022

SARS-CoV-2 cases have increased to 463,182,124 reported worldwide as of March 16, 2022 with a death toll of 6,079,600. Indonesia on March 17, 2022 has increased by 5,939,082 with a death toll of 153,212 (KPCPEN, 2022). SARS-CoV-2 is closely related to the coronavirus. One of the efforts made to prevent the risk of severe infection and reduce the death rate from the SARS-CoV-2 virus in Indonesia is a vaccination program. According to IDI (2021) cases of Covid-19 deaths are dominated by men (84%) and women (16%). Clinical trials in China and Brazil have shown that, the immunogenicity of two doses of the vaccine decreases over time and there is a decrease in low antibody concentrations after 6 months of running time. To overcome this potential, the Indonesian government gives priority to the 3rd vaccine booster. The purpose of this study was to analyze the comparison between the total antibody titer for the Spike-RBD (Receptor Binding Domain) SARS-CoV-2 protein after the 3rd dose of mRNA-1273 booster vaccination and post-vaccination of two doses of primary inactivated whole virus in health workers. This research was conducted by observation with a cohort approach. It was concluded that the results of the Mann Whitney two-sided test with a significance value of 0.000, the antibody titer correlation test results with gender had a value of 0.702 and -0.366 for the relationship between antibody titer and age. There was a significant difference between the primary vaccination dose of 2 doses of CoronaVac and the 3rd vaccination (booster) mRNA-1273, gender was strongly associated with S-RBD antibody titer and age was not associated with S-RBD antibody titer.
Isolation and Frustul Characterization of Diatom Navicula sp. TAD Strain
Authors: Telussa Ivon , Rahayu  
Published: December 2022

Frustul diatom Navicula sp. galur TAD diisolasi melalui proses pencucian dengan asam dan pembakaran. Sel Navicula sp. galur TAD dikultivasi sel selama 7 hari pada temperature ruang di bawah intensitas cahaya 5000 lux untuk mendapatkan kerapatan sel dan biomassa yang tinggi. kerapatan sel tertinggi diperoleh sebesar 72,167 × 105 ± 0,946 sel mL-1 .biomassa kering diperoleh sebesar 11,459 g dengan produktivitas biomassa sebesar 0,1364 gL-1 h -1. Frustul Navicula sp. galur TAD hasil pencucian HCl 20% dan pembakaran pada 600 oC memiliki warna frustul yang lebih putih. Karakterisasi frulstul menunjukkan struktur dengan nanopori pada permukaan frustul terlihat jelas. Frustul dari Navicula sp galur TAD tersusun atas silikon dan oksigen
Hubungan antara Pengetahuan, Perilaku Ergonomi Fisik Siswa Sma saat Belajar dan Kejadian Nyeri Punggung pada Siswa SMA Jakarta Barat
Authors: Setiyowati Yovita Dwi , Hartati Yohana Rani 
Published: December 2022

Low back pain is a musculoskeletal disorder caused by poor sitting posture. The incident of low back pain among students are effected by knowledge, computer use, and sitting posture.The purpose of this study was known the relationship among knowledge, physical ergonomic behaviour, and low back pain incidence at Senior High School Jakarta. This research used a quantitative methode with descriptive correlative research design and cross-sectional approach. The total sample was 114 students with purposive sampling technique. The result use Chi-Square test showed that knowledge was significant association (p <0,05) with ergonomic behaviour, and ergonomic behaviour was significant asociation with low back pain incidence (p-value = 0.000).
GENERAL ASPECTS OF STUDYING HYPERTEXTS AS AN OBJECT OF LEXICO-SEMANTIC ANALYSIS
Authors: Novyk Anna 
Published: December 2022

The article is devoted to the problems of hypertext research as an object of lexical-semantic analysis. The article defines the concept, essence and features of hypertexts. Special attention is paid to lexical and semantic characteristics of hypertexts, as well as defining features of historical realities in hypertexts on the material of the electronic encyclopedia Wikipedia. It was established that in some cases it is inappropriate to use a literal translation of historical realities in hypertexts, as this affects the logical structure of the sentence and the adequacy of the translation as a whole.
Keywords: hypertexts; link; electronic hypertexts; translation; lexical and semantic characteristics.
STRUCTURAL AND SEMANTIC FEATURES OF ENGLISH LANGUAGE ECOMARKETING TERMS
Authors: Tikan Yana , Melnychuk Alina 
Published: December 2022

This article is devoted to the study of structural, semantic features of translation of ecomarketing terms in English-language texts. The study of terms that relate to the topic of ecomarketing and serve to express concepts in the field of this activity is carried out. The ecomarketing term system is an ordered set of terms that express a system of ecomarketing concepts. Ecomarketing is an applied, practical, descriptive discipline, complex and has an intermediate character. The paper reveals the meaning of the concept of "ecomarketing", defines methods for creating terms of ecomarketing, determines the relevance of the research topic and further prospects for its development. Relationships between different terminological systems have different forms: the use of the term one branches in scientific texts of another branchб and inclusion of the term of one branch in the scientific term system of another industries. The term system of modern ecomarketing, a system that is constantly being improved and considered ahead. Synonymy in the term system is a manifestation of a common language patterns.
Keywords: structural and semantic features; methods of term formation; term; terminology of ecomarketing; terminological word-units.
A Semi-Analytical Model of an Off-Center Multi-Wing Fractured Well in a Low-Permeability Gas Reservoir
Authors: Yang Sihan , Li Xiaoping , Liu Qiguo , Xu Youjie 
Published: December 2022

Summary
Since there are several hydraulic fractures around a wellbore after a large-scale hydraulic fracturing and the well is not in the center of the reservoir, no corresponding semianalytical model for wellbore pressure analysis has been proposed. To bridge this gap, this paper aims to present a semianalytical model of the off-center multiwing fractured well. With consideration of permeability stress sensitivity, the reservoir model and hydraulic fracture model are established, respectively. The coupling approach of the reservoir model and hydraulic fracture model is used to obtain the wellbore pressure solution. Meanwhile, the off-center multiwing fractured well is verified with a numerical solution. The seven flow regimes can be distinguished according to the characteristics of the pressure derivative curve. Furthermore, the effect of different fracture distributions on wellbore pressure and the derivative curve is discussed and analyzed. Assuming that the fracture wing number is equal to the average length of all fracture wings, the wellbore pressure is lowest before the radial flow regime when the fracture wing has a uniform distribution around the angle and all fracture wings are equal in length. Besides, the influence of other important parameters (fracture wing number, off-center distance, etc.) is discussed. According to the analysis, we conclude that fracture wing number has a significant influence on the pressure and derivative curves before the radial flow regime. The off-center distance has no influence on the pressure and derivative curve before the radial flow regime, but it has an obvious influence on arc boundary reaction time. Finally, the advantages of the semianalytical solution are fast calculation speed and high calculation accuracy (especially in the early flow regime).
Multiplex Immunohistochemistry protocol in formalin-fixed, paraffin embedded Colorectal Liver Metastases patient tissues and Machine Learning- based Tissue Segmentation and Cell Phenotyping analysis&nbsp;
Authors: Vallejo-Ardila Dora L. , Tutuka Candani S. A. , Fifis Theodora , Walsh Katrina A. , Muralidharan Vijayaragavan , Christophi Christopher , Perini Marcos V. 
Published: December 2022

Abstract
Colorectal cancer liver metastasis (CLM) is the most of common cause of death in patients with colorectal cancer (CRC) worldwide. There are various immune-suppressive and tumor-promoting mechanisms, which contribute to the unresponsiveness associated with current check point immunotherapy in CRC. Techniques such as Multiplex immunohistochemistry (mIHC) and automated image quantitation analysis, enable a deeper understanding of the diversity of the host’s anti-tumor immune response and could identify potential targets for immunotherapy. Herein, we present a 7-plex OPALTM protocol used to assess the composition and spatial distribution of T cell markers CD3, CD8, Foxp3 and CD103 and the epithelial to mesenchymal transition (EMT) markers alpha smooth muscle actin (α-SMA) and E-cadherin. The protocol has been manually optimized and validated in two independent cohorts of formalin-fixed, paraffin embedded CLM patient tissues (n=42) using well-established antibodies, single spectral library, negative controls and biological controls for corroborating staining pattern of α-SMA+ (sclerosed hemangioma samples) and T-cell infiltrates (benign liver). The accurate profiling of T- cell composition, location and phenotypic characterization could reveal important insights about the influence of T lymphocytes on prognosis after liver metastasectomy.
Stem cell-derived mouse embryos develop within an extra-embryonic yolk sac to form anterior brain regions and a beating heart-like structure.
Authors: Amadei Gianluca , Handford Charlotte E. , Glover David , Zernicka-Goetz Magdalena 
Published: December 2022

Abstract
Mouse embryos are an invaluable tool to study mammalian development but establishment of novel transgenic mouse lines is a costly and time-consuming endeavour. Stem cell models, on the other hand, lend themselves to quick genetic manipulation and are easily scalable, yet they do not successfully capture morphogenesis.Here, we report stem cell-based structures called ETiX-embryoids that can recapitulate mouse post-implantation morphogenesis. ETiX-embryoids form by aggregating embryonic and trophoblast stem cells to embryonic stem cells transiently expressing Gata4. Over the course of 4 days, these structures develop like mouse post-implantation egg cylinders; they then undertake and complete gastrulation on day 5 and 6, respectively. On day 7, ETiX-embryoids undergo neural induction and form the mouse anterior-posterior axis from neural folds to tail bud by movements of convergent extension. On day 8 they form a brain, establish a beating heart-like structure and a gut tube, as they develop inside extraembryonic membranes equivalent to amnion and yolk sac. We detail here the experimental set up and procedure required to generate ETiX-embryoids, and anticipate that they will establish themselves as a novel, powerful tool to study mammalian development without generating a novel mouse lines.
Shifting consumers towards sustainable food consumption and avoiding food waste: Protocol for a machine-learning assisted systematic review and meta-analysis of demand-side interventions
Authors: Lohmann Paul M. , Pizzo Alice , Carvalho Bruna , Bauer Jan Michael , Reisch Lucia A. 
Published: December 2022

Abstract
It is now widely accepted that a significant portion of emissions reductions required to meet net zero targets must come from individual behaviour change. Shifting consumers towards more sustainable food consumption and avoiding food waste and loss (FWL) have been identified as two key levers to tackle climate change at the individual and household level. While the IPCC estimates substantial “technical potential” to reduce emissions via changes in diets and reductions in FWL, there is a lack of learning on which climate solutions can best harness this potential. The purpose of this systematic review and meta-analysis is to synthesise the empirical literature reporting on demand-side interventions targeting sustainble food consumption and food waste behaviours of individuals and households. The review encompasses empirical research evaluating a wide range of policy interventions targeted at changing actual food consumption and waste behaviours, which have the potential to contribute towards climate change mitigation. The review forms part of an ‘ecosystem of reviews’, a large-scale evidence synthesis initiative seeking to provide a comprehensive analysis of household-scale interventions and their emissions reduction potential. The reviews within the ecosystem utilise state-of-the-art AI-assisted screening procedures and follow a set of harmonised inclusion criteria.
Single-Cell B-cell Sequencing to Generate Natively-paired scFab Yeast Surface Display Libraries
Authors: Pascual Nathaniel , Belecciu Theodore , Schmidt Sam , Nakisa Athar , Huang Xuefei , Woldring Daniel 
Published: December 2022

Abstract
The immune cell profiling capabilities of single-cell RNA sequencing (scRNA-seq) are powerful tools that can be applied to the design of theranostic monoclonal antibodies (mAbs). Using scRNA-seq to determine natively-paired B-cell receptor (BCR) sequences of immunized mice as a starting point for design, this method outlines a simplified workflow to express single-chain antibody fragments (scFabs) on the surface of yeast for high-throughput characterization and further refinement with directed evolution experiments. While not extensively detailed in this chapter, this method easily accommodates the implementation of a growing body of in silico tools that improve affinity and stability among a range of other developability criteria (e.g., solubility, immunogenicity).
A Cross- sectional Study on Bovine Tuberculosis in Small holder Intensive Dairy Farms at Assosa District, Western Ethiopia
Authors: Disassa Haimanot , Mekonnen Nega 
Published: December 2022

Abstract
Background: Bovine tuberculosis (BTB) is a contagious chronic and debilitating disease of cattle that can infect humans, other domestic animal and some wild life. Assosa district is one of the remote settings in Ethiopia where information on neglected tropical disease like BTB is not well documented. Hence this study aimed to estimate the prevalence of bovine tuberculosis at Assosa District, Western Ethiopia.
Results: For the current study, 224 dairy cows were sampled and screened for bovine tuberculosis. Among which 18 (8.04%) cows were found positive for bovine tuberculosis where as the rest were negative for the screening test. The effects of different associated risk factors (like age, breed type, and body condition score) for the occurrence of BTB were investigated. The difference in reactivity to the CIDT test among the study participants in different age groups, breed types and body condition were found statistically significant (P-value = 0.034, 0.002 and 0.000) respectively showing higher risk of BTB in older animals when compared to the younger ones (OR=6.42, 95% CI, .01-0.20), exotic breeds (OR=7.49, 95% CI, 2.10-26.78) and poor body condition scored animals (OR=16.75, 95% CI, 5.41-51.8).
Conclusion: The prevalence reported in the current study only using CIDT test revealed that BTB is present and is well-established in small holder dairy farms in the district. The farm owners in the study areas are usually in close contact with their animals and also consume raw milk regularly which may predispose them for the disease. Thus, the livestock agency of the district and the researchers should give awareness creation to the farm owners on the relevance of screening test for BTB before purchasing the animals. Moreover, further detailed epidemiological as well as molecular studies should be done to investigate the link between bovine and human tuberculosis in the study area in order to design appropriate strategic prevention and control measures.
WITHDRAWN: Pulmonary nodules and the psychological distress they can cause: A scoping review
Authors: Milano Nicole , Bulman William A. , Hillyer Grace Clarke 
Published: December 2022

Abstract
Background: More than 1.5 million solitary pulmonary nodules are diagnosed in the United States each year. While the majority prove to be benign, there is evidence to suggest that any incidental and lung cancer screening-detected pulmonary nodules cause patients psychological distress/anxiety.
Purpose: To identify the nature and extent of the evidence, we conducted a scoping review of the literature related to patient nodule-specific psychological distress/anxiety.
Findings: Of the eight studies identified, five assessed nodule-specific anxiety quantitatively and three qualitatively. Quantitative anxiety constructs included nodule-specific distress (n=3; Impact of Events Scale), anxiety alone (n = 1; Generalized Anxiety Disorder-7 measure); and anxiety and depression combined (n = 2; Hospital Anxiety and Depression Scale). Constructs examined qualitatively included emotional distress (n = 2) and anxiety, fear, and worry (n = 1). Most studies evaluated other factors such as lung cancer risk perceptions and quality of patient/provider communication. Pulmonary nodule-specific anxiety was recorded in >50% of the participants (mild 23%-27%; moderate to severe 16%-24%). Anxiety was associated with financial concerns related to a possible cancer diagnosis, fear/worry about cancer, lack of understanding of pulmonary nodules, and pre-existing depression or PTSD. Anxiety was diffused with better patient/provider communication and active coping strategies and tended to decline to the subclinical level over time.
Conclusions: Our review suggests that many patients experience nodule-specific anxiety. These findings serve as a call to action forthe development of interventions to mitigate nodule-specific anxiety andto improve the quality of the patient experience when pulmonary nodules are discovered.