bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-452 negatively regulates osteoblast differentiation in periodontal ligament stem cells by targeting the polycomb-group protein, BMI1
Authors: Mao Tiantian , Li Jun , Peng Ruobing , Liu Linhua , Peng Youjian 
Published: January 2022

Purpose: To determine whether miR-452 regulates osteoblast differentiation (OD) in human periodontal ligament stem cells (hPDLSCs) by targeting polycomb-group protein BMI1.
Methods: hPDLSCs were stimulated to differentiate upon treatment with mineralization liquid. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were used to measure mRNA and protein expressions, respectively. Alkaline phosphatase (ALP) activity and Alizarin red staining were used to determine the osteogenic differentiation (OD) of hPDLSCs. The bioinformatics software, Targetscan, was used to predict the potential target of miR-452, while luciferase assay, qRT- PCR, and western blot were employed to verify the target gene of miR-452, BMI1.
Results: MiR-452 was downregulated during the OD of hPDLSCs, but miR-452 overexpression inhibited the OD of hPDLSCs. BMI1 was identified as a direct target gene of miR-452 during the OD of hPDLSCs, while miR-452 overexpression correlated inversely with BMI1 expression during OD of hPDLSCs.
Conclusion: Overexpression of miR-452 suppresses the OD of hPDLSCs by targeting BMI1.This study may provide potential diagnostic and therapeutic basis for OD in hPDLSCs.
Antioxidant and anti-inflammatory activities of Oroxylum indicum Kurz (L.) fruit extract in lipopolysaccharide-stimulated BV2 microglial cells
Authors: Mairuae Nootchanat , Cheepsunthorn Poonlarp , Buranrat Benjaporn 
Published: January 2022

Purpose: To investigate the anti-inflammatory effects and antioxidant activity of Oroxylum indicum (L.) Kurz fruit extract in lipopolysaccharide (LPS)-stimulated BV2 microglia.
Methods: BV2 cells were treated with LPS for 24 h in the presence or absence of O. indicum fruit extract. Then, nitric oxide (NO), reactive oxygen species (ROS) and interleukin 6 (IL-16) levels were measured using Griess reagent assay, CM-H2DCFDA and enzyme-linked immunosorbent (ELISA) assays, respectively. The in vitro antioxidant property of the extract was also investigated by 1,1- diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assays.
Results: Levels of IL-6, NO, and ROS in LPS-treated BV2 cells were significantly higher than those in control (p < 0.01). However, exposure of LPS-treated BV2 cells to O. indicum extract led to a marked decrease in the levels of these parameters, when compared to the untreated cells (p < 0.01). Results from DPPH and ABTS assays showed that the O. indicum extract exhibited good antioxidant properties, with total flavonoid and total phenolic contents of 115.58 ± 1.09 and 131.04 ± 2.37 mg/g of dried extract, respectively.
Conclusion: The results demonstrate that O. indicum fruit exerts anti-oxidant and anti-inflammatory effects in LPS-stimulated BV2 cells. Thus O. indicum fruit might be beneficial in the development of novel anti-oxidative and anti-neuroinflammatory herbal medicines. However, the mechanisms by which O. indium fruits reduces NO and IL-6 needs to be further investigated.
Effects of bee propolis on thyroid function tests in healthy volunteers
Authors: Alassaf Fawaz A. , Qazzaz Mohannad E. , Alfahad Mohanad , Abed Mohammed N. , Jasim Mahmood H.M. , Thanoon Imad A.J. 
Published: January 2022

Purpose: The current study investigates the effects of bee propolis as a dietary supplement on thyroid function in healthy individuals.
Methods: Sixty-nine apparently healthy subjects of both genders aged between 25 and 45 years and had a body mass index in the range of 18.5-25 kg m-2 were enrolled in the study. The participants were divided into two groups, 34 volunteers treated with 1 gram of bee propolis daily and the other 35 were on placebo pills for 2 months. Total triiodothyronine (TT3), total thyroxine (TT4) and ultra-sensitive thyroid stimulating hormone (USTSH) were measured in the two groups before and after treatment to assess thyroid function.

Results: The results showed a significant increase in body weight (from 73.4 ± 7.2 to 74.8 ± 7.5 kg, p < 0.05) and BMI (from 24.2 ± 1.2 to 24.7 ± 1.5 kg m-2 , p < 0.05) with significantly decreased TT3 (from 2.17 ± 0.07 to 1.87 ± 0.09 nmol/L, p < 0.01) in bee propolis-treated group, whereas TT4 and USTSH showed non-significant changes.
Conclusion: The results reveal a significant association of bee propolis supplementation with reduced TT3 levels in healthy subjects. The link may assist clinicians in monitoring the safety of bee propolis supplementation.
Iontophoresis-assisted pediatric transdermal delivery of ropivacaine to enhance anesthetic effect
Authors: Yu Liqun , Liu Yanna , Sang Miao 
Published: January 2022

Purpose: To formulate novel transdermal patches of ropivacaine (RPV) and enhance its anesthetic activity using iontophoresis technique.
Methods: Solvent casting method was used for the development of the transdermal patches with the aid of hydroxypropyl methylcellulose (HPMC) and polyvinyl alcohol (PVA) polymers. The patches were characterized for appearance, clarity, smoothness, thickness, average weight of patches, folding endurance, RPV uniformity, moisture absorption and tensile strength. The anesthetic effect of ropivacaine patches were evaluated in a healthy pediatric population divided into two groups. One group was merely applied with the patch while the other group received the patch using LidoSite applicator. The anesthetic effect was assessed based on hot pain, cool sensation and warm sensation.
Results: The patches were smooth and transparent, about 0.29 mm in thickness, having a tensile strength of 0.51 kg/cm2 and showed in-vitro drug release of 68.94 to 96.83 %. The formulation did not exert any undesirable local skin effects, and produced minimum pain intensity. The depth of anesthesia was higher for the patch formulation with iontophoresis than for the conventional system.
Conclusion: The findings point to the superiority of RPV transdermal patches when applied with iontophoretic device as against the conventional application.
Complications and cost estimations in herpes zoster – a retrospective analysis at a Swiss tertiary dermatology clinic
Authors: Bongers Leonard D. , Navarini Alexander , Berger Christoph T. , Mueller Simon M. 
Published: January 2022

INTRODUCTION: Herpes zoster is the common reactivation of latent varicella zoster virus infection and is associated with substantial morbidity and costs. Age, immunosuppression, diabetes and active cancer are important risk factors for developing herpes zoster. The recombinant herpes zoster vaccine is highly effective in preventing this infection. To inform cost-effectiveness analyses of vaccination in risk groups, it is important to define the rates of complications, mortality, and hospitalisation and its costs.
METHODS: In this single-centre, investigator-initiated, retrospective study we analysed herpes zoster patients treated in our tertiary dermatology referral centre between 2005 and 2019. Case costs were calculated using health insurance invoices and by estimating the drug-related costs. The associations between patients' characteristics and complications were assessed using logistic regression models.
RESULTS: A total of 355 herpes zoster patients were analysed (mean age 61.5 ± 18.8 years; 56.6% women). The complication rate was 30.1% (n = 107) with post-herpetic neuralgia being the most frequent (37/355, 10.4%). The herpes zoster-related hospitalisation rate was 19.2% (n = 68), the herpes zoster-related mortality rate 0.85% (n = 3). Of all patients, 22.8% (n = 81) had herpes zoster risk factors. The odds ratio for complications (multivariate analysis) was 2.5 (95% confidence interval [CI] 1.12–5.72) in cancer patients and 1.04 (1.02–1.05) for each additional year of age (1.41 for 10 years). The cost ratio (median) of in- vs outpatient treatment was 26.9 (CHF/case 9029 vs 335), of all complicated vs non-complicated herpes zoster patients 6.6 (CHF/case 2203 vs 332) and of complicated vs non-complicated herpes zoster outpatients 4.3 (CHF/case 1331 vs 306).
CONCLUSION: In this herpes zoster population at a university hospital, one in three patients was affected by herpes zoster-related complications. Age and cancer were independently associated with a high complication rate, suggesting a need to vaccinate cancer patients. Herpes zoster-related complications and the need for inpatient treatment escalated the costs per case.
PELATIHAN PENULISAN ARTIKEL ILMIAH HASIL PENELITIAN BAGI GURU
Authors: Ulfa Siti Maria , Aisyah Rasyidah Nur , Setyowati Yulis 
Published: January 2022

Melakukan penelitian tidak semudah menulis artikel. Menulis sebuah artikel dari hasil penelitian mungkin menjadi sesuatu yang sangat sulit bagi mereka yang belum pernah mengenalnya. Dalam menyusun susunan bagaimana sebuah artikel harus dibuat atau disusun guru perlu disesuaikan dengan cara bagaimana mereka seharusnya bekerja melakukan sebuah artikel. Melalui workshop online ini, tim pengabdi mencoba membantu para guru agar terbiasa menyusun sebuah penelitian menjadi sebuah artikel yang siap dipublikasikan. Kegiatan ini dilakukan secara online di mana terlebih dahulu guru diberikan penjelasan bagaimana seharusnya sebuah artikel dibuat. Setelah itu, mereka dibagi menjadi beberapa kelompok dengan tutornya untuk membantu mereka mengerjakan lokakarya. Tujuan dari kegiatan ini adalah untuk memberikan dan menambah wawasan pengetahuan tentang bagaimana menulis karya ilmiah utamanya menyusun artikel dari hasil penelitian. Metode yang dipakai dalam kegiatan ini adalah metode ceramah, diskusi, tanya jawab dan penyampaian materi. Hasilnya, salah seorang guru berhasil menyusun penelitiannya menjadi sebuah artikel yang siap dipublikasikan. Workshop ini sangat membantu para guru untuk membuka pikiran mereka tentang bagaimana artikel yang bagus dapat dibuat dari penelitian mereka sendiri. Sebagai kesimpulan dari kegiatan ini adalah bahwa pelatihan ini adalah hal yang memang dinantikan oleh semuanya walaupun kenyataannya menulis karya ilmiah adalah hal yang cukup berat mengingat guru dengan faktor usia dan pekerjaan sekolah lainnya. Maka perlu adanya kegiatan sejenis yang berkelanjutan dan terarah
PELATIHAN INTERNET SEHAT DAN AMAN UNTUK REMAJA
Authors: Nelfianti Fera , Martiwi Rini , Rahman Arif , Kurniawan Aris 
Published: January 2022

Teknologi Informasi semakin berkembang pesat. Hal ini menyebabkan siapa saja dapat mengakses internet dengan mudah. Bagi anak-anak usia sekolah internet tentunya menjadi bagian dari kehidupannya sehari-hari baik yang tinggal di daerah maupun di perkotaan atau yang bisa kita kenal dengan generasi digital. Generasi ini dinamakan generasi digital karena dari mereka lahir sudah kenal dengan berbagai macam perangkat digital. Generasi ini akan memanfaatkan internet dengan cara yang positif tentu saja banyak sekali hal-hal dan informasi baik yang didapat seperti dalam bidang pendidikan, pengetahuan yang tersedia dari seluruh platform yang sangat mudah diakses. Selain itu juga anak-anak dapat memanfaatkan internet untuk melatih otak dengan permainan-permainan yang edukatif. Pemanfaatan internet juga sebagai bagian dari komunikasi, sebut saja electronic mail, atau aplikasi-aplikasi pesan instan yang dapat mudah di unduh di internet. Selain itu juga internet digunakan sebagai media untuk terhubung dan bersosialisasi dengan cara daring, seperti aplikasi facebook, Instagram, youtube dan lain-lain. Seiring dengan perkembangan kemajuan dari teknologi internet dan kemudahan dalam mengakses data, maka tentu saja ancaman negatif dari internet sangatlah dekat. Mudahnya mengakses konten-konten pornografi, cyberbullying, judi, dan pelecahan pun tentu saja menjadi ancaman yang menakutkan yang mempengaruhi remaja saat ini, hal tersebut menjadi alasan perlunya diadakan pendekatan, pemahaman dan sosliasisasi penggunaan internet yang baik sehingga dapat mengatasi bahaya yang ada. Adapun manfaat yang diperoleh dari kegiatan Pengabdian Masyarakat melalui pelatihan Internet Sehat dan Aman Untuk Remaja di bawah binaan Panti Wisma Karya Bakti bisa dilihat dari hasil posttest pengetahuan mereka tentang internet sehat. Menurut peserta pelatihan, dengan diadakannya pelatihan ini mereka mendapatkan manfaat yang cukup besar sehingga mengetahui Batasan-batasan dalam mengakses internet. Para peserta awalnya menganggap link-link yang beredar dapat dengan mudah di akses tanpa ada bahaya konten, dengan pelatihan ini, para peserta lebih berhat-hati lagi dalam mengakses informasi yang ada di internet
MENINGKATKAN CITRA BUAH KESEMEK MENJADI BUAH LAYAK SUPERMARKET
Authors: Pasla Peter R Y , Taneo Stefanus Y M , Kunjaya Chatif , Wicaksono Soetam Rizky 
Published: January 2022

Buah kesemek memiliki kandungan gizi yang tinggi serta kaya akan vitamin dan zat-zat lain yang bermanfaat bagi kesehatan. Rasa buah kesemek yang matang sebenarnya manis sekali, lebih manis dari apel, namun buah ini getahnya terasa lengket dan sangat mengganggu di mulut. Untuk menetralkan getah kesemek, para petani memeramnya di dalam air kapur selama beberapa hari. Ketika diangkat dari air kapur, sisa kapur mengering di kulit buah membuat kesemek nampak seperti dilapisi bedak putih. Karena bedaknya itu, kesemek terkesan kotor sehingga dijual dengan harga murah di pasar tradisional. Program ini bertujuan meningkatkan citra buah kesemek menjadi buah layak supermarket dengan cara menghapuskan kesan kotor buah kesemek karena penggunaan kapur. Dengan menggunakan gas CO2 sebagai ganti air kapur dihasilkan buah kesemek yang bersih, higienis dan layak dipasarkan di super-market dengan harga yang bersaing dengan harga buah-buahan modern lainnya. Buah kesemek diuji-pasarkan di tiga supermarket yang berbeda dengan perlakuan yang berbeda dengan harga 2 kali lipat harga kesemek yang dijual di pasar tradisional. Di supermarket pertama buah kesemek dijual dengan menyediakan gerai khusus dengan dilayani langsung oleh tim yang aktif mempromosikan buah kesemek tersebut kepada pengunjung super-market menggunakan brosur dan personal selling. Di supermarket kedua, buah kesemek dijual tanpa perlakuan khusus dan diletakkan bercampur dengan buah-buah lain di lokasi penjualan buah tanpa upaya promosi namun tetap ditempatkan beberapa anggota tim untuk melayani pengunjung. Di supermarket ketiga buah kesemek dijual tanpa didampingi tim dan upaya promosi. Hasilnya adalah di supermarket pertama dalam jangka 1 minggu buah kesemek sebanyak 100 Kg terjual habis. Di supermarket kedua buah kesemek sebanyak 50 Kg selama 1 minggu terjual 30 Kg. Di supermarket ketiga buah kesemek sebanyak 10 Kg tidak dapat terjual.Kata Kunci: Kesemek, Kapur, Gas CO2, supermarket
Analisis Sistem Pendukung Keputusan Penentuan Kasi Terbaik Menerapkan Metode OCRA dengan Pembobotan Rank Order Centroid (ROC)
Authors: Mayadi Mayadi , Pamungkas R Wisnu Prio , Azlan Azlan , Khairunnisa Khairunnisa , Waruwu Fince Tinus 
Published: January 2022

Abstrak−Kasi (Kepala Seksi) pada suatu pemerintahan desa merupakan unsur pelaksana operasional pemerintahan yang dipimpin seorang kepala seksi. Peran kasi sangatlah dibutuhkan dalam meningkatkan kualitas pemerintahan desa. Dalam penentuan Kasi terbaik haruslah memenuhi beberapa kriteria seperti Lama Bekerja, Pengabdian Masyarakat, Loyalitas Kerja, Usia dan Pendidikan Terakhir. Maka dari itu dibutuhkan suatu Sistem Pendukung Keputusan (SPK) untuk mengatasi masalah tersebut menggunakan metode Operational Competitiveness Rating Analysis (OCRA) untuk memperoleh data-data yang dibutuhkan dalam menentukan kriteria Kasi terbaik. Sehingga dalam penelitian penentuan Kasi terbaik ini, menghasilkan alternatif A5 dengan nilai 1.347 sebagai alternatif terbaik.
Kata Kunci: Sistem Pendukung Keputusan; Kasi; Metode OCRA
Abstract−Kasi (Section Head) in a village government is an element of implementing government operations led by a section head. The role of the cation is very much needed in improving the quality of village governance. In determining the best Kasi must meet several criteria such as Length of Work, Community Service, Work Royality, Age and Last Education. Therefore, a Decision Support System (DSS) is needed to overcome this problem using Operational Competitiveness Rating Analysis (OCRA) to obtain the data needed to determine the best Kasi criteria. So that in this research to determine the best Kasi, it produces alternative A5 with a value of 1,347 as the best alternative.
Keywords: Decision Support System; Kasi; OCRA Method
Ownership-Retention and The Value of Indonesian Initial Public Offering (IPO) Firms in 2013-2016
Authors: Hendrawaty Ernie , Adlina Syifa Fadiah 
Published: January 2022

<p><em>The study investigates information asymmetry between security issuers and other market participants. Investors and entrepreneurs require reliable information about the firms. Therefore, it is crucial to examine how the top management information can be communicated to other market participants. The empirical implications of the model are tested on the set of 28 of 89 Indonesian firms that made an initial public offering in 2013-2016. The asymmetry problem is most significant when a firm makes a first public issue. Furthermore, the study explains the number of shares retained by the entrepreneur as a signal of IPO firm value. The test results are generally consistent with the signaling model's predictions, while the role of the controlling variables does not support the firm value.</em></p><p><strong><em>Keywords: </em></strong><em>Ownership-Retention, Firm</em> <em>Value, Initial Public Offering, IPO</em></p><p><em> </em></p>
Refined Pythagorean Fuzzy Sets: Properties, Set-Theoretic Operations and Axiomatic Results
Authors: Saeed Muhammad , Ahmad Muhammad Rayees , Rahman Atiqe Ur 
Published: January 2022

Refined Pythagorean fuzzy set is the generalization of fuzzy set, intuitionistic fuzzy set, Pythagorean fuzzy set, refined fuzzy set, and refined intuitionistic fuzzy set as it not only bears the characteristics of these models but also addresses their limitations for real-life scenarios with sub-grades corresponding to membership and non-memberships grades. In order to equip the existing literature with conceptual framework of refined Pythagorean fuzzy sets, essential properties, set-theoretic operations, and axiomatic results are characterized with the help of numerical examples.

Received: 22 December 2021 | Revised: 22 January 2022 | Accepted: 25 January 2022

Conflicts of Interest
The authors declare that they have no conflicts of interest to this work.
Similarity-Distance Decision-Making Technique and its Applications via Intuitionistic Fuzzy Pairs
Authors: Ejegwa Paul Augustine , Agbetayo Johnson Mobolaji 
Published: January 2022

The idea of intuitionistic fuzzy sets (IFSs) is a reasonable soft computing construct for resolving ambiguity and vagueness encountered in decision-making situations. Cases such as pattern recognition, diagnostic analysis, etc., have been explored based on intuitionistic fuzzy pairs via similarity-distance measures. Many similarity and distance techniques have been proposed and used to solve decision-making situations. Though the existing similarity measures and their distance counterparts are somewhat significant, they possess some weakness in terms of accuracy and their alignments with the concept of IFSs, which needed to be strengthened to enhance reliable outputs. As a consequent, this paper introduces a novel similarity-distance technique with better performance rating. A comparative analysis is presented to showcase the advantages of the novel similarity-distance over similar existing approaches. Some attributes of the similarity-distance technique are presented. Furthermore, the applications of the novel similarity-distance technique in sundry decision-making situations are explored.

Received: 8 December 2021 | Revised: 14 January 2022 | Accepted: 15 January 2022

Conflicts of Interest
The authors declare that they have no conflicts of interest to this work.
Influence of organic aerosol molecular composition on particle absorptive properties in autumn Beijing
Authors: Cai Jing , Wu Cheng , Wang Jiandong , Du Wei , Zheng Feixue , Hakala Simo , Fan Xiaolong , Chu Biwu , Yao Lei , Feng Zemin , Liu Yongchun , Sun Yele , Zheng Jun , Yan Chao , Bianchi Federico , Kulmala Markku , Mohr Claudia , Daellenbach Kaspar R. 
Published: January 2022

Abstract. Organic aerosol (OA) is a major component of fine particulate matter (PM),
affecting air quality, human health, and the climate. The absorptive and
reflective behavior of OA components contributes to determining particle
optical properties and thus their effects on the radiative budget of the
troposphere. There is limited knowledge on the influence of the molecular
composition of OA on particle optical properties in the polluted urban
environment. In this study, we characterized the molecular composition of
oxygenated OA collected on filter samples in the autumn of 2018 in Beijing,
China, with a filter inlet for gases and aerosols coupled to a
high-resolution time-of-flight chemical ionization mass spectrometer
(FIGAERO–CIMS). Three haze episodes occurred during our sampling period with
daily maximum concentrations of OA of 50, 30, and 55 µg m−3. We found that the signal intensities of dicarboxylic acids and
sulfur-containing compounds increased during the two more intense haze
episodes, while the relative contributions of wood-burning markers and other
aromatic compounds were enhanced during the cleaner periods. We further
assessed the optical properties of oxygenated OA components by combining
detailed chemical composition measurements with collocated particle light
absorption measurements. We show that light absorption enhancement
(Eabs) of black carbon (BC) was mostly related to more oxygenated OA
(e.g., dicarboxylic acids), likely formed in aqueous-phase reactions during
the intense haze periods with higher relative humidity, and speculate that
they might contribute to lensing effects. Aromatics and nitro-aromatics
(e.g., nitrocatechol and its derivatives) were mostly related to a high light
absorption coefficient (babs) consistent with light-absorbing (brown)
carbon (BrC). Our results provide information on oxygenated OA components at
the molecular level associated with BrC and BC particle light absorption and
can serve as a basis for further studies on the effects of anthropogenic OA
on radiative forcing in the urban environment.