bluuebunny/crossref_metadata_2025_split huggingface dataset:
"I have the right to determine how I die": A Qualitative Research on Chinese Cancer Patients' attitudes on Death and Demands for Good Death
Authors: Cao Zhaoming , Zheng Jie , Ding Jingjing , Zhang Huijun 
Published: December 2022

Abstract
Objectives In order to expand palliative care in China and improve the quality of life and death of Chinese cancer patients, the purpose of this research is to investigate the attitudes of Chinese cancer patients toward death, as well as their requirements and expectations of a good death.
MethodsPhenomenological research was used to conduct in-depth interviews and data analysis on the topic of death and good death of 16 cancer patients.
Results Cancer patients' attitudes towards death can be divided into three types: open-faced, negative and pessimistic, complicated psychology of acceptance and hope; The demand for good death includes four themes and nine sub-themes: physiological needs (to relieve physical pain), psychological needs (to choose autonomy, to maintain dignity, to satisfy wishes, family hopes, and arrangements for matters related to death), spiritual needs (inner comfort), and social needs (financial situation, medical system support).
Conclusion Medical staff should deeply understand the cognition and attitude of cancer patients towards death, guide patients to express their dying feelings and physical and mental needs, and strive to seek the cooperation of patients' families and other professionals, such as lawyers and psychotherapists, so as to meet patients' expectations of good death at all levels. China should increase the training of medical staff in knowledge and skills of good death, set up palliative medical institutions and improve relevant laws, safeguard patients' rights and help patients achieve good death.
Effects of a passive back assistance exoskeleton for load carrying and trunk bending tasks
Authors: Thomas ALBOUY , Kévin LEBEL , Bérenger LE TELLIER 
Published: December 2022

Abstract
Low back pain affects millions of workers every year. From a preventive perspective, the use of passive exoskeletons is growing in the industrial field. The present study aims to evaluate the effects of a passive trunk exoskeleton on muscle activity and kinematics of the trunk and legs, displacement of the center of pressure, and perceived discomfort. 14 participants performed two tasks with and without exoskeleton. The first one was a lifting and lowering task and the second one was a static trunk flexion task at 40°. The results showed a significant reduction of approximately 18% in myoelectric activity of the erector spinae longissimus as well as a reduction in perceived discomfort in the thoracolumbar area on both tasks. The use of the exoskeleton increased external oblique activity by 28.6% during the load-bearing task, while leg muscles were not significantly impacted. The exoskeleton slightly impacted the kinematics of the trunk but leg kinematics were not, nor was postural balance. Given the study’s results, this device therefore seems to have the ability to reduce the risk of onset of low back pain at work.
The development of selenium-rich millet via soil fertilization and the interactions among microbial community, plants and rhizosphere soil
Authors: Wei Dong , Gao Huiya , Zhang Aijun 
Published: December 2022

Abstract
Selenium is an important microelement for both plants and human health. The deficiency of selenium would result in various diseases and has attracted much attention. In modern agriculture, different strategies have been adopted for selenium biofortification. In this study, selenium soil fertilization was applied at different levels to develop selenium-rich millet. External supplementation of selenium showed positive effects on plant growth and nutrient transport. The activities of key enzymes of plant and soil were observed to enhance after selenium fertilization, such as glutathione peroxidase (GSH-Px), sucrose, and urease. The major objective of obtaining selenium-rich millet grain successfully achieved as the final selenium content in grain increased by 5–10 folds. Middle level of selenium showed the best performance among all the treatments. The analysis of microbial community in rhizosphere soil suggested the good adaptation of both bacteria and fungi toward environmental conditions modified by fertilization.
What’s in a Smile? The features of Indigenous Fijians’ and NZ Europeans’ smiles
Authors: Reginald Kumar , Mohammed Hisham , Firth Fiona , Bennani Hamza , Halberstadt Jamin B , Farella Mauro 
Published: December 2022

Abstract
The Fijian “Bula Smile,” is often described as the world’s friendliest, however its description remains anecdotal. We aim to describe and compare the features of Fijians’ smiles with those of New Zealand Europeans. An observational study was conducted on two ethnic groups, Fijians (FJ; N=23) and New Zealand Europeans (NZ; N=23), age- and gender-matched. All participants were asked to watch amusing videos, and their reactions were video recorded. The videos were analysed by software to assess the frequency, duration, intensity, and genuineness of smiling episodes. Based on the Facial Action Coding System, Action Unit 6 (AU6 - cheek raiser), Action Unit 12 (AU12 - lip corner puller), and Action Unit 25 (AU25 – lips apart) were assessed. Data were analysed by generalised linear models after adjusting for personality traits. Fijians smiled longer than New Zealand Europeans (+19.9%; p=0.027). Mean intensity of AU6 (+1.0; 95%CIs=0.6-1.5; p<0.001), AU12 (+0.5; 95%CIs=0.1-0.9; p=0.008) and AU25 (+22.3%; 95%CIs=7.3-37.3%; p=0.005) were significantly higher in FJ group than the NZ group. Smiling features of Fijians and New Zealanders showed objective differences, the most distinctive being a higher activation of the Duchenne’s marker (AU6) in the Fijian group, which is regarded as a sign of smile genuineness.
Realtime observation of ATP-driven single B12 molecule translocation through BtuCD-F
Authors: Hunt John , Zhu Lingwei , Kim Jinrang , Leng Kun , Ramos John , Kinz-Thompson Colin , Karpowich Nathan , Gonzalez Ruben 
Published: December 2022

Abstract
ATP-Binding Cassette (ABC) Transporters use ATP binding and hydrolysis to power transmembrane transport of chemically diverse substrates. Current knowledge of their mechanism comes primarily from static structures of stable intermediates along the transport cycle. Recently, single-molecule fluorescence resonance energy transfer (smFRET) measurements have generated insight into the functional dynamics of transmembrane transporters, but studies to date lack direct information on the physical movement of the transport substrate. Here, we report development of an smFRET system that exploits fluorescence quenching by vitamin B12 to track its location in real time during ATP-driven transport by nanodisc-reconstituted E. coli BtuCD-F, an extensively studied type II ABC importer. Our data demonstrate that transmembrane translocation of B12 is driven by two sequential high-energy conformational changes that are inaccessible to standard structural methods because they are inherently transient. The first moves B12 from the periplasm into the transmembrane domain of the transporter; notably, this reaction is driven by hydrolysis of a single ATP molecule, in contrast to the mechanism established for several other ABC Transporter families in which ATP-binding drives the mechanochemical power-stroke prior to hydrolysis. The second mediates B12 release on the opposite side of the transporter, and it is driven by formation of a hyper-stable complex between BtuCD and BtuF. Hydrolysis of a second single ATP molecule is then required to dissociate BtuCD from the BtuF substrate-binding protein to enable it to bind B12 and initiate another round of transport. Our experiments have visualized substrate translocation in real-time at a single-molecule level and provided unprecedented information on the mechanism and dynamics of a paradigmatic transmembrane transport process.
The role of testicular microcirculatory disorders in spermatogenic dysfunction in obese men
Authors: Fu Wei , Cui Jun , Tang Shaoshan 
Published: December 2022

Abstract
Background: Obesity is a recognized risk factor for systemic microcirculatory disorders. The association between obesity-related microcirculatory disorders and spermatogenesis has been difficult to investigate and has not been reported in the literature until now. The aim of this study was to study the role of testicular microcirculatory disorders in spermatogenic dysfunction in obese men.
Subjects: 576 participants were enrolled in this prospective observational study.
Methods: Angio PLUSTM Planwave Ultrasensitive Imaging of the testicular microcirculation was performed and the number of vessels was counted and recorded as the ultrasonic microvascular density (UMVD).
Results: Participants were divided into Group I (normozoospermia), Group II (asthenozoospermia, teratozoospermia and asthenoteratozoospermia) and Group III (oligozoospermia and NOA) based on semen results. There were no differences in BMI and UMVD between semen groups (p>0.05). Participants were then divided into normal weight, overweight and obese subgroups based on body mass index. In the obese subgroup, UMVD tended to decrease with decreasing sperm concentration, with significant differences in groups I, II and III (p<0.01). ROC curve for UMVD was established for differential diagnosis between Groups I, II and Group III in the obese subgroup. UMVD had a high diagnostic value with a cut-off value of 19.25 /cm2, AUC of 0.829, sensitivity of 0.773 and specificity of 0.737 (95% CI: 0.740-0.917).
Conclusions: Our findings suggest that microcirculatory disorders play an essential role in the development of spermatogenic dysfunction in obese men. Obese participants with oligozoospermia and NOA had a significantly lower UMVD. Testicular UMVD below 19.25 /cm2 was highly suggestive of spermatogenic dysfunction. Angio PLUSTM Planwave Ultrasensitive Imaging should be used routinely to screen testicular microcirculation in obese men and to identify those who may benefit from microcirculation improvement therapy.
Simulating the impact of projected climate on growth and yield of wheat in Eastern India using crop growth simulation model
Authors: Banerjee Saon , Sattar Abdus , Kumari P , MA Sarath Chandran 
Published: December 2022

Abstract
Crop simulation models offer a great tool for understanding the impact of climate change factors, their interaction and effect on crop growth and yield. It is essential to ensure higher wheat yield, being a major crop during the winter season on Eastern India, to achieve the food security in coming years. The present study aims to assess the impact of projected climate (rcp 4.5 of IPCC-AR5) on grain yield and biomass of wheat at three locations under three different States of Eastern India using CERES-Wheat model under DSSAT (v4.7). The impact of projected climate on wheat yield and biomass was studied using output of global climate model GFDL-ESM2G for the periods 2030-31, 2060-61 and 2090-91, with and without increase in CO2 concentration. The results indicated that grain yield in Kalyani is projected to be higher in future periods under reduced moisture depletion treatments, grain yield in Ranchi is projected to decrease under early sowing condition and the response is mixed at Samastipur. Across the locations, the effect of future CO2 concentration was observed and the reduction in grain yield for future was less when CO2 level enhancement was considered. Simple adaptation options, like higher dose of nutrient application (e.g. 160: 80: 80 kg per ha N: P: K) and irrigation management have the capacity to retain the yield plateau under changing climatic scenario of Mohanpur. Similarly, sowing of wheat during second fortnight of December in Samastipur region can reduce the adverse impact of climate change.
Fully Automated Agatston score calculation from ECG gated Cardiac CT using Deep learning and Multiorgan Segmentation: A Validation study
Authors: Gautam Ashish , Raghav Prashant , Subramanya Vijay , Kumar Sunil , Kumar Sudeep , Jain Dharmendra , Verma Ashish , Singh Parminder , Singhal Manphoul , Gupta Vikash , Rathore Samir , Iyengar Srikanth , RATHORE SUDHIR 
Published: December 2022

Abstract
Purpose: To evaluate deep learning-based calcium segmentation and quantification on ECG gated Cardiac CT scans compared with manual evaluation.
Methods:Automated calcium quantification was performed using a combination of deep-learning convolution neural networks based on Mask R CNN for multiorgan segmentation. Calcifications were identified automatically, after which the algorithm automatically excluded all non-coronary calcifications using 2D erosion, dilation, volume, and maximum intensity threshold and by applying cardiac, aortic, and epicardial fat segmentation. This study used 40 patients to train and test the segmentation model.
Results:110 patients were tested for the validation of the algorithm. The Pearson correlation coefficient between the reference actual and the computed predictive scores on the test set show high level of correlation (0.84; p < 0.001) and high limits of agreement in Bland-Altman plot. The proposed method correctly classifies the risk group in 75.2% of the cases and classifies the subjects in the same group. 81% of the predictive scores lie in the same categories and only seven patients out of 110 were more than one category off. For the presence/absence of coronary artery calcifications, the deep learning model achieved a sensitivity of 90 % and a specificity of 94 %.
Conclusion: Fully automated deep learning-based calcium quantification on cardiac-CTs shows good correlation compared to reference standards. Automating this process may reduce evaluation time and potentially optimize clinical calcium scoring without additional resources.
The speed of propagation of background radiation
Authors: Tokarev Valerii , Kuleshov Gennadii 
Published: December 2022

Abstract
We have experimentally found that the speed of propagation of the relict background radiation is 123 km/s. The obtained result is confirmed by the excessively large width of its autocorrelation function, which is possible only if the signal propagation speeds before and after reflection from the mirror of an offset parabolic pointed antenna differs by several orders of magnitude. In addition, the width of the autocorrelation function of the signal received from the blue shift of the background radiation spectrum and from the red shift differs by 2.4 times, which is possible only if the total speed of the Earth's motion in the Galaxy is comparable to the speed of the propagation of relict radiation. The low rate of propagation of the relict radiation leads to a significant expansion of its correlation function due to refraction in the presence of clouds and other inhomogeneities in the atmosphere, which is absolutely absent when receiving satellite television broadcast signals under exactly the same conditions and is an indicator of the cosmological cause of the detected anomalies.
PACS: 98.80.-k
Determining KLF13 structure, uniqueness, and possible molecular crosstalk in Prostate Cancer
Authors: Khan Khushbukhat , Gulzar Atia , Badshah Yasmin , Ashraf Naeem Mahmood , Rafiq Mehak , Hamid Arslan , Shabbir Maria , Afsar Tayyaba , Almajwal Ali , Arshad Mohammed , Razak Suhail 
Published: December 2022

Abstract
Background: The mortality rate of prostate cancer is rising rapidly, and its early diagnosis greatly impacts the success rate of its treatment. Despite the identification of numerous biomarkers, its timely identification has been compromised, which calls for the need to identify new markers for its timely diagnosis. KLF13 is a transcription factor that regulates the transcription of several oncogenic proteins, specifically KRAS and Akt pathway proteins. Its aberrant expression is previously found in cancers. Therefore, in this study, the structure and cellular localization of KLF13 were predicted, and its interaction with sin3A was studied through protein-protein docking and molecular dynamics simulations. Furthermore, TPD52 and PKCɛ were identified as the targets of KLF13, and PKCɛ as a target for miR-223.
Methods:KLF13 aberrant expression impact on TPD52, PKCɛ, and miR-223 was also studied through real time-PCR, and a signalling cascade associated with prostate carcinogenicity was constructed.
Results:The study outcomes indicated the nuclear localization of KLF13 and the presence of the sin3A binding domain. KLF13-sin3A interaction analysis showed hydrophobic interactions dominate KLF13 and Sin3A binding, and KLF13 binding with Sin3A is transient. Blood-based expression analysis revealed that TPD52 expression was highly up-regulated, while expression of miR-223, PKCɛ, and KLF13 was downregulated compared to healthy controls.
Conclusion: This outcome has potential application in modulating KLF13 activity in several diseases. The study further delineated the differential expression of these proteins and their role in PCa, which provided new insights into the potential mechanisms of oncogenesis and metastasis of PCa and may facilitate in designing of novel diagnostic strategies.
Finding inhibitor from phytochemicals for novel target Glycosyltransferase family 62 protein in Trichophyton rubrum using insilico study
Authors: Hussain Syed Abuthakir Mohamed , Elangovan Nandha Devi , Malik Abdul , Khan Mohammad , Muthusamy Jeyam 
Published: December 2022

Abstract
The dermatophyte Trichophyton rubrum is producing more than 70% of dermatophytosis in human and animals. Glycosyltransferase family 62 protein in T.rubrum is potential and novel drug target which is non-homologous to human, human gut microbiota and it is not targeted by any drug. It is very essential for priming mannosyltransferase activity and different types of N-glucan biosynthesis. Various parts of medicinal plant Balanites aegyptiaca are used in treating many diseases in human especially skin diseases. Aim of this study is to find potential inhibitor from phytochemicals of various medicinal plant sources against the novel drug target. 3D structures of Glycosyltransferase family 62 protein was obtained by homology modeling and docked with the compounds from phytochemicals of various plant species using GLIDE and best pose of docked complex free energy was calculated by MM-GBSA analysis using PRIME. The stability of the best docked complex was evaluated by molecular dynamics simulation studies using Desmond module of Schrödinger. Cyanidin 3-O-rhamnoside had better result with novel target Glycosyltransferase family 62 protein of T.rubrum which has to be further assessed in vitro and in vivo.
The Effect of Water Extract of Betaone against Osteoporosis in OVX mice by Inhibiting MAP Kinase Signaling
Authors: Lee Yongjin , Lee Hyun-Jin , Kim Kwang-Jin , Shin Han-Byeol , Ham Ju Ri , Lee Mi-Ja , Lee Mi-Kyung , Son Young-Jin 
Published: December 2022

Abstract
Betaone barley (Hordeum vulgare) is one of the most important crops and have detoxification, antioxidant, and cholesterol control effects. To analyze the effect of Betaone water extract (B1W) on osteoclasts, mRNA and protein expression of nuclear factor-activated T cells c1 (NFATc1) were measured. The mRNA expression of several proteins involved in osteoclast differentiation were investigated, and bone pit assays to investigate bone resorption function were performed. Finally, micro-CT imaging of ovariectomized (OVX) mice was performed to investigate in vivo activity. The results showed that B1W reduced the transcription and translation expression of NFATc1, and decreased mRNA expression levels of various marker genes and reduced the osteoclast activity. Moreover, improvement effects of B1W were obtained in osteoporosis-induced mice. Based on the results, B1W is considered a useful candidate as a therapeutic agent for treating bone diseases such as osteoporosis, and could also be used as an ingredient in health supplements.
One Novel BiP/GRP78 Inhibitor YUM70 Induces GSDME Dependent Pyroptosis and Enhances Sensitivity To EGFR Inhibitors in cholangiocarcinoma and hepatocellular carcinoma
Authors: Sun Lei , Liu Yanan , Zhou Hao , Zhang Mingyan , Cao XueLei , Zhang Jian 
Published: December 2022

Abstract
Cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) are deadly malignancy with poor prognosis and limited treatment options. Endoplasmic reticulum (ER) stress plays an important role in the pathogenesis and development of malignant solid tumors which is associated with chemotherapeutic drug resistance. The therapeutic potential of targeting ER stress signaling in cancer via surface BiP/GRP78 (78-kDa glucose-regulated protein), a major role in ER stress sensing, is now under clinical trials. YUM70 is a novel inducer of ER stress that induces apoptosis in cancer by directly bound BiP and inactivated its function. In this study, we investigated the possible role of epidermal growth factor receptor (EGFR) pathway and cell death mechanisms in YUM70 induced CCA or HCC cells cytotoxicity. Although both YUM70 and HA15 as BiP inhibitors exerted the mono-therapeutic anti-proliferation effect and induced autophagy and apoptosis, YUM70 exhibited more potent anti-tumor potential by suppressing the EGFR downstream signaling: ERK1/2 and mTOR/p70(S6K) pathways at the concentration of 100 µM more effectively. At the same tested concentration, HA15 could not inhibit the phosphorylation of ERK1/2 or p70(S6K). Moreover, we discovered that YUM70 induced GSDME dependent pyroptosis by activating NF-κB pathway and inhibited EMT via inactivation of β-catenin pathway. Additionally, pharmacologic targeting of ERK signaling is usually limited by adaptive resistance, frequently mediated by feedback activation of receptor tyrosine kinases (RTKs) signaling. We observed that treatment of HuCCT1 or Huh7 cells with YUM70 resulted in increased EGFR phosphorylation. Inhibiting EGFR activation with Gefitinib or Osimertinib synergistically increased the anti-tumor activity of BiP inhibitors. Our results demonstrated novel strategy that BiP inhibitors, in combination with Gefitinib or Osimertinib, should be tested in CCA or HCC patients.
Information asymmetric non-cooperative game and virtual games of players
Authors: Chen Jeanpantz 
Published: December 2022

Abstract
An important assumption in classical non-cooperative game theory is that players have complete information or nearly complete information, but in real games, information is often asymmetric among the players. Undoubtedly, in an information asymmetric non-cooperative game, players hypothesize their own virtual games on the basis of their own information sets, and thus decide their own strategic choices. This paper provides a virtual game model of an information asymmetric non-cooperative game, and proposes a proof of the existence of the solution to the virtual game of an arbitrary player.
In an information asymmetric non-cooperative game, the signals issued by a certain player can change the information sets of other players, thus affect their strategic choices and change his own expected payoff. In this paper the author also analyzes the optimal signal strategy of a player.