bluuebunny/crossref_metadata_2025_split huggingface dataset:
International Survey on Frailty Assessment in Patients with Cancer
Authors: Banna Giuseppe Luigi , Cantale Ornella , Haydock Maria Monica , Battisti Nicolò Matteo Luca , Bambury Kevin , Musolino Naja , O’Carroll Eoin , Maltese Giuseppe , Garetto Lucia , Addeo Alfredo , Gomes Fabio 
Published: July 2022

Abstract

Background
Frailty negatively affects the outcomes of patients with cancer, and its assessment might vary widely in the real world. The objective of this study was to explore awareness and use of frailty screening tools among the ONCOassist healthcare professionals (HCPs) users.

Materials and Methods
We sent 2 emails with a cross-sectional 15-item survey in a 3-week interval between April and May 2021. Differences in the awareness and use of tools according to respondents’ continents, country income, and job types were investigated.

Results
Seven hundred thirty-seven HCPs from 91 countries (81% physicians, 13% nurses, and 5% other HCPs) completed the survey. Three hundred and eighty-five (52%) reported assessing all or the majority of their patients; 518 (70%) at baseline and before starting a new treatment. Three hundred and four (43%) HCPs were aware of performance status (PS) scores only, 309 (42%) age/frailty/comorbidity (AFC) screening, and 102 (14%) chemotoxicity predictive tools. Five hundred and thirty-seven (73%) reported using tools; 423 (57%) just PS, 237 (32%) AFC, and 60 (8%) chemotoxicity ones. Reasons for tools non-use (485 responders) were awareness (70%), time constraints (28%), and uselessness (2%). There were significant differences in awareness and use of screening tools among different continents, country income, job types, and medical specialties (P &lt; .001 for all comparisons).

Conclusion
Among selected oncology HCPs, there is still a worldwide lack of knowledge and usage of frailty screening tools, which may differ according to their geography, country income, and education. Targeted initiatives to raise awareness and education are needed to implement frailty assessment in managing patients with cancer.
SlMBP3 Knockout/down in Tomato: Normal-Sized Fruit with Increased Dry Matter Content through Non-Liquefied Locular Tissue by Altered Cell Wall Formation
Authors: Kim Ji-Seong , Lee Jeongeun , Ezura Hiroshi 
Published: July 2022

Abstract
The phenotypic effect of the knockdown/out of AGAMOUS clade MADS-box gene SlMBP3 in tomato was evaluated using a transferred DNA (T-DNA)-tagged mutant of SlMBP3 and SlMBP3-RNA interference lines. SlMBP3 was preferentially expressed in the locular tissue of fruit and the seed coat combined with the endoderm. Consistent with where SlMBP3 is expressed, the SlMBP3-knockout/down lines showed non-liquefied locular tissues and increased number of seed hairs than the wild type (WT). The early cell degradation of the locular tissue was not observed in the fruits of the SlMBP3-knockout/down lines, and the cells were elongated like placental cells resulting in non-liquefied locular tissues. As the result, the fruits of the SlMBP3-knockout/down lines exhibited higher dry matter contents and titratable acidity than those of the WT. During locular tissue cell development under the SlMBP3 knockout/down, the expression of cell-enlargement-related genes (beta-expansin gene SlEXPB1 and endo-beta-1,4-D-glucanase gene Cel8) and pectinase-inhibitor-related genes (pectin esterase inhibitor gene PE inhibitor and polygalacturonase inhibitor gene PG inhibitor) was upregulated and that of pectinase-encoding genes (polygalacturonase gene QRT3-like and pectin lyase gene PL2) was downregulated. In the seed coat of the SlMBP3-knockout/down lines, tomato trichome-formation-related genes such as MYB genes containing R2 and R3 repeats (R2R3-MYB) transcription factor SlMYB75, B-type cyclin SlCycB2 and Homeodomain Leucine Zipper (HD-Zip) IV transcription factor Woolly were downregulated. Our results demonstrate that SlMBP3 is involved in the liquefaction of the locular tissue through the modification of cell development and degradation processes and seed hair formation in tomato fruits, and the SlMBP3 knockout/down results in normal-sized fruit with increased dry matter content.
Deep origin and gradual evolution of transporting tissues: Perspectives from across the land plants
Authors: Woudenberg Sjoerd , Renema Jim , Tomescu Alexandru M F , De Rybel Bert , Weijers Dolf 
Published: July 2022

Abstract
The evolution of transporting tissues was an important innovation in terrestrial plants that allowed them to adapt to almost all nonaquatic environments. These tissues consist of water-conducting cells and food-conducting cells and bridge plant–soil and plant–air interfaces over long distances. The largest group of land plants, representing about 95% of all known plant species, is associated with morphologically complex transporting tissue in plants with a range of additional traits. Therefore, this entire clade was named tracheophytes, or vascular plants. However, some nonvascular plants possess conductive tissues that closely resemble vascular tissue in their organization, structure, and function. Recent molecular studies also point to a highly conserved toolbox of molecular regulators for transporting tissues. Here, we reflect on the distinguishing features of conductive and vascular tissues and their evolutionary history. Rather than sudden emergence of complex, vascular tissues, plant transporting tissues likely evolved gradually, building on pre-existing developmental mechanisms and genetic components. Improved knowledge of the intimate structure and developmental regulation of transporting tissues across the entire taxonomic breadth of extant plant lineages, combined with more comprehensive documentation of the fossil record of transporting tissues, is required for a full understanding of the evolutionary trajectory of transporting tissues.
The advantage of the right in social media news sharing
Authors: González-Bailón Sandra , d'Andrea Valeria , Freelon Deen , De Domenico Manlio 
Published: July 2022

AbstractWe analyze social media activity during one of the largest protest mobilizations in US history to examine ideological asymmetries in the posting of news content. Using an unprecedented combination of four datasets (tracking offline protests, social media activity, web browsing, and the reliability of news sources), we show that there is no evidence of unreliable sources having any prominent visibility during the protest period, but we do identify asymmetries in the ideological slant of the sources shared on social media, with a clear bias towards right-leaning domains. These results support the “amplification of the right” thesis, which points to the structural conditions (social and technological) that lead to higher visibility of content with a partisan bent towards the right. Our findings provide evidence that right-leaning sources gain more visibility on social media and reveal that ideological asymmetries manifest themselves even in the context of movements with progressive goals.
Constructive adaptation of 3D-printable polymers in response to typically destructive aquatic environments
Authors: Yu Kunhao , Feng Zhangzhengrong , Du Haixu , Lee Kyung Hoon , Li Ketian , Zhang Yanchu , Masri Sami F , Wang Qiming 
Published: July 2022

Abstract
In response to environmental stressors, biological systems exhibit extraordinary adaptive capacity by turning destructive environmental stressors into constructive factors; however, the traditional engineering materials weaken and fail. Take the response of polymers to an aquatic environment as an example: Water molecules typically compromise the mechanical properties of the polymer network in the bulk and on the interface through swelling and lubrication, respectively. Here, we report a class of 3D-printable synthetic polymers that constructively strengthen their bulk and interfacial mechanical properties in response to the aquatic environment. The mechanism relies on a water-assisted additional cross-linking reaction in the polymer matrix and on the interface. As such, the typically destructive water can constructively enhance the polymer’s bulk mechanical properties such as stiffness, tensile strength, and fracture toughness by factors of 746% to 790%, and the interfacial bonding by a factor of 1,000%. We show that the invented polymers can be used for soft robotics that self-strengthen matrix and self-heal cracks after training in water and water-healable packaging materials for flexible electronics. This work opens the door for the design of synthetic materials to imitate the constructive adaptation of biological systems in response to environmental stressors, for applications such as artificial muscles, soft robotics, and flexible electronics.
Plasmonic gadolinium oxide nanomatryoshkas: bifunctional magnetic resonance imaging enhancers for photothermal cancer therapy
Authors: Henderson Luke , Neumann Oara , Kadria-Vili Yara , Gerislioglu Burak , Bankson James , Nordlander Peter , Halas Naomi J 
Published: July 2022

Abstract
Nanoparticle-assisted laser-induced photothermal therapy (PTT) is a promising method for cancer treatment; yet, visualization of nanoparticle uptake and photothermal response remain a critical challenge. Here, we report a magnetic resonance imaging-active nanomatryoshka (Gd2O3-NM), a multilayered (Au core/Gd2O3 shell/Au shell) sub-100 nm nanoparticle capable of combining T1 MRI contrast with PTT. This bifunctional nanoparticle demonstrates an r1 of 1.28 × 108 mM–1 s–1, an MRI contrast enhancement per nanoparticle sufficient for T1 imaging in addition to tumor ablation. Gd2O3-NM also shows excellent stability in an acidic environment, retaining 99% of the internal Gd(3). This report details the synthesis and characterization of a promising system for combined theranostic nanoparticle tracking and PTT.
Resolving the lineage relationship between malignant cells and vascular cells in glioblastomas
Authors: Wang Fangyu , Liu Xuan , Li Shaowen , Zhao Chen , Sun Yumei , Tian Kuan , Wang Junbao , Li Wei , Xu Lichao , Jing Jing , Wang Juan , Evans Sylvia M , Li Zhiqiang , Liu Ying , Zhou Yan 
Published: July 2022

Abstract
Glioblastoma multiforme (GBM), a highly malignant and heterogeneous brain tumor, contains various types of tumor and non-tumor cells. Whether GBM cells can trans-differentiate into non-neural cell types, including mural cells or endothelial cells, to support tumor growth and invasion remains controversial. Here we generated two genetic GBM models de novo in immunocompetent mouse brains, mimicking essential pathological and molecular features of human GBMs. Lineage tracing and transplantation studies demonstrated that, although blood vessels in GBM brains underwent drastic remodeling, evidence of trans-differentiation of GBM cells into vascular cells was barely detected. Intriguingly, GBM cells could promiscuously express markers for mural cells during gliomagenesis. Furthermore, single-cell RNA sequencing showed that patterns of copy-number variations (CNVs) of mural cells and endothelial cells were distinct from those of GBM cells, indicating discrete origins of GBM cells and vascular components. Importantly, single-cell CNV analysis of human GBM specimens also suggested that GBM cells and vascular cells are likely separate lineages. Rather than expansion owing to trans-differentiation, vascular cell expanded by proliferation during tumorigenesis. Therefore, cross-lineage trans-differentiation of GBM cells is very unlikely to occur during gliomagenesis. Our findings advance understanding of cell lineage dynamics during gliomagenesis, and have implications for targeted treatment of GBMs.
Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm
Authors: Liu Kuisheng , Xu Xiaocui , Bai Dandan , Li Yanhe , Zhang Yalin , Jia Yanping , Guo Mingyue , Han Xiaoxiao , Liu Yingdong , Sheng Yifan , Kou Xiaochen , Zhao Yanhong , Yin Jiqing , Liu Sheng , Chen Jiayu , Wang Hong , Wang Yixuan , Liu Wenqiang , Gao Shaorong 
Published: July 2022

Abstract
Self-organized blastoids from extended pluripotent stem (EPS) cells possess enormous potential for investigating postimplantation embryo development and related diseases. However, the limited ability of postimplantation development of EPS-blastoids hinders its further application. In this study, single-cell transcriptomic analysis indicated that the “trophectoderm (TE)-like structure” of EPS-blastoids was primarily composed of primitive endoderm (PrE)-related cells instead of TE-related cells. We further identified PrE-like cells in EPS cell culture that contribute to the blastoid formation with TE-like structure. Inhibition of PrE cell differentiation by inhibiting MEK signaling or knockout of Gata6 in EPS cells markedly suppressed EPS-blastoid formation. Furthermore, we demonstrated that blastocyst-like structures reconstituted by combining the EPS-derived bilineage embryo-like structure (BLES) with either tetraploid embryos or tetraploid TE cells could implant normally and develop into live fetuses. In summary, our study reveals that TE improvement is critical for constructing a functional embryo using stem cells in vitro.
The impact of Italian performance-based research funding systems on the intensity of international research collaboration
Authors: Abramo Giovanni , D’Angelo Ciriaco Andrea 
Published: July 2022

Abstract
The study of national research assessment exercises serves to evaluate the effectiveness of policies versus their objectives and to improve the formulation of future initiatives. The aim of the current study is to verify whether the introduction of the first performance-based research funding in Italy, based on the 2004–10 VQR assessment, achieved the intended objective of inducing greater international collaboration on the part of researchers. For this, we apply a bibliometric approach based on the observation of coauthorships in Italian and worldwide scientific publications over a 14-year period, beginning in the target years of the VQR assessment. Through an Interrupted Time Series Analysis, we compare the expected and observed patterns of international coauthorship for Italy and the rest of the world. Although the rate of internationalization of Italian research is seen to be increasing, whether this is a consequence of the VQR incentives, or rather part of a global phenomenon of recourse to international collaboration in response to the increasingly complex scientific challenges, it is open to interpretation.
Routinization of work processes, de-routinization of job structures
Authors: Fernández-Macías Enrique , Bisello Martina , Peruffo Eleonora , Rinaldi Riccardo 
Published: July 2022

Abstract
This article investigates changes in routine tasks and computer use in European jobs in the period 1995–2015, putting them in the context of the debates on the future of work and the impact of automation. Digital technologies not only affect employment shifts but also shape work organization. A shift-share analysis combining European Working Conditions Survey and European Labour Force Survey data assesses to what extent recent changes in tasks are the result of changes in the structure of employment (shifts in employment across jobs) or changes in the content of work itself (transformation in the task contents and methods within jobs). The results suggest contrasting trends between observed changes in tasks measures within jobs and compositional shifts in employment for routine tasks indexes. Employment structures are de-routinizing while work itself is becoming more routine. These results seem also related to the increased use of computers at work during the same period.
Identification of novel loci in obstructive sleep apnea in European American and African American children
Authors: Quinlan Courtney M , Chang Xiao , March Michael , Mentch Frank D , Qu Hui-Qi , Liu Yichuan , Glessner Joseph , Sleiman Patrick M A , Hakonarson Hakon 
Published: July 2022

Abstract

Study Objectives
To identify genetic susceptibility variants in pediatric obstructive sleep apnea in European American and African American children.

Methods
A phenotyping algorithm using electronic medical records was developed to recruit cases with OSA and control subjects from the Center for Applied Genomics at Children’s Hospital of Philadelphia (CHOP). Genome-wide association studies (GWAS) were performed in pediatric OSA cases and control subjects with European American (EA) and African American (AA) ancestry followed by meta-analysis and sex stratification.

Results
The algorithm accrued 1486 subjects (46.3% European American, 53.7% African American). We identified genomic loci at 1p36.22 and 15q26.1 that associated with OSA risk in EA and AA, respectively. We also revealed a shared risk locus at 18p11.32 (rs114124196, p = 1.72 × 10-8) across EA and AA populations. Additionally, association at 1q43 (rs12754698) and 2p25.1 (rs72775219) was identified in the male-only analysis of EA children with OSA, while association at 8q21.11 (rs6472959), 11q24.3 (rs4370952) and 15q21.1 (rs149936782) was detected in the female-only analysis of EA children and association at 18p11.23 (rs9964029) was identified in the female-only analysis of African-American children. Moreover, the 18p11.32 locus was replicated in an EA cohort (rs114124196, p = 8.8 × 10-3).

Conclusions
We report the first GWAS for pediatric OSA in European Americans and African Americans. Our results provide novel insights to the genetic underpins of pediatric OSA.
The Community Coevolution Model with Application to the Study of Evolutionary Relationships between Genes Based on Phylogenetic Profiles
Authors: Liu Chaoyue , Kenney Toby , Beiko Robert G , Gu Hong 
Published: July 2022

Abstract
Organismal traits can evolve in a coordinated way, with correlated patterns of gains and losses reflecting important evolutionary associations. Discovering these associations can reveal important information about the functional and ecological linkages among traits. Phylogenetic profiles treat individual genes as traits distributed across sets of genomes and can provide a fine-grained view of the genetic underpinnings of evolutionary processes in a set of genomes. Phylogenetic profiling has been used to identify genes that are functionally linked and to identify common patterns of lateral gene transfer in microorganisms. However, comparative analysis of phylogenetic profiles and other trait distributions should take into account the phylogenetic relationships among the organisms under consideration. Here, we propose the Community Coevolution Model (CCM), a new coevolutionary model to analyze the evolutionary associations among traits, with a focus on phylogenetic profiles. In the CCM, traits are considered to evolve as a community with interactions, and the transition rate for each trait depends on the current states of other traits. Surpassing other comparative methods for pairwise trait analysis, CCM has the additional advantage of being able to examine multiple traits as a community to reveal more dependency relationships. We also develop a simulation procedure to generate phylogenetic profiles with correlated evolutionary patterns that can be used as benchmark data for evaluation purposes. A simulation study demonstrates that CCM is more accurate than other methods including the Jaccard Index and three tree-aware methods. The parameterization of CCM makes the interpretation of the relations between genes more direct, which leads to Darwin’s scenario being identified easily based on the estimated parameters. We show that CCM is more efficient and fits real data better than other methods resulting in higher likelihood scores with fewer parameters. An examination of 3786 phylogenetic profiles across a set of 659 bacterial genomes highlights linkages between genes with common functions, including many patterns that would not have been identified under a nonphylogenetic model of common distribution. We also applied the CCM to 44 proteins in the well-studied Mitochondrial Respiratory Complex I and recovered associations that mapped well onto the structural associations that exist in the complex. [Coevolution; evolutionary rates; gene network; graphical models; phylogenetic profiles; phylogeny.]
Prediction Model for Severe Mycoplasma pneumoniae Pneumonia in Pediatric Patients by Admission Laboratory Indicators
Authors: Chang Qing , Chen Hong-Lin , Wu Neng-Shun , Gao Yan-Min , Yu Rong , Zhu Wei-Min 
Published: July 2022

Abstract

Objective
The purpose of this study was to develop a model for predicting severe Mycoplasma pneumoniae pneumonia (SMPP) in pediatric patients with Mycoplasma pneumoniae pneumonia (MPP) on admission by laboratory indicators.

Methods
Pediatric patients with MPP from January 2019 to December 2020 in our hospital were enrolled in this study. SMPP was diagnosed according to guideline for diagnosis and treatment of community-acquired pneumonia in children (2019 version). Prediction model was developed according to the admission laboratory indicators. Receiver operating characteristic curve and Goodness-of-fit test were analyzed for the predictive value.

Results
A total of 233 MPP patients were included in the study, with 121 males and 112 females, aged 4.541 (1–14) years. Among them, 84 (36.1%, 95% CI 29.9–42.6%) pediatric patients were diagnosed as SMPP. Some admission laboratory indicators (immunoglobulins M (IgM), eosinophil proportion, eosinophil count, hemoglobin, erythrocyte sedimentation rate (ESR), total protein, albumin and prealbumin) were found statistically different (p &lt; 0.05) between non-SMPP group and SMPP group. Logistic regress analysis showed IgM, eosinophil proportion, eosinophil count, ESR and prealbumin were independent risk factors for SMPP. According to these five admission laboratory indicators, the prediction model for SMPP in pediatric patients was developed. The area under curve of the prediction model was 0.777, and the goodness-of-fit test showed that the predicted SMPP incidence by the model was consistent with the actual incidence (χ2 = 244.51, p = 0.203).

Conclusion
We developed a model for predicting SMPP in pediatric patients by admission laboratory indicators. This model has good discrimination and calibration, which provides a basis for the early identification SMPP on admission. However, this model should be validated by multicenter studies with large sample.
Treatment outcomes and risk factors of death in childhood tuberculous meningitis in Kandahar, Afghanistan: a prospective observational cohort study
Authors: Rahimi Bilal Ahmad , Niazi Najeebullah , Rahimi Ahmad Farshad , Faizee Muhammad Ishaque , Khan Mohmmad Sidiq , Taylor Walter R 
Published: July 2022

Abstract

Background
Tuberculous meningitis (TBM) is the most severe form of TB. We prospectively documented the treatment outcomes and the risk factors for death in children with TBM from Kandahar, Afghanistan.

Methods
This prospective observational cohort study was conducted from February 2017 to January 2020 in hospitalised TBM children. All the patients were prospectively followed up for 12 mo. Data were analysed by using descriptive statistics, χ2 and multivariate logistic regression.

Results
A total of 818 TBM hospitalised patients with median age 4.8 (0.8–14.5) y were recruited. Females accounted for 60.9% (498/818). Upon admission 53.9% (n=441) and 15.2% (n=124) had TBM stages II and III, respectively, and 23.2% (n=190) had focal neurological signs. The case fatality rate was 20.2% (160/794) and 30.6% (243/794) survived with neurological sequelae. Independent risk factors for death were being unvaccinated for BCG (adjusted OR [AOR] 3.8, 95% CI 1.8 to 8.1), not receiving dexamethasone (AOR 2.5, 95% CI 1.5 to 4.2), being male (AOR 2.3, 95% CI 1.5 to 3.6), history of recent weight loss (AOR 2.2, 95% CI 1.3 to 3.9) and having stage III TBM (AOR 2.0, 95% CI 1.2 to 3.3).

Conclusions
TBM continues to cause high morbidity and mortality in Afghan children. Strategies to reduce mortality should emphasise early diagnosis and treatment, routine use of dexamethasone and increased BCG vaccination.