bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of non-mechanical bowel preparation on postoperative gastrointestinal recovery after surgery of gynaecological malignant tumours: A randomised controlled trial
Authors: Wang ShanShan , Xu Hongyan , Li XingXia , Feng Suwen 
Published: August 2022

Abstract
Objective:To investigate the efcacy and safety of Non-mechanical bowel preparation in patients undergoing surgery of gynaecological malignant tumour.Methods: Patients (n=105) were randomized to receive mechanical bowel preparation（MBP）or Non-mechanical bowel preparation (Non-MBP). The primary outcomes were postoperative gastrointestinal function. Secondary outcomes included Postoperative complaints and plasma of D-lactate and diamine oxidase(DAO). Visualisation of the surgical field, involuntary defecation during surgery, operation time, wound healing, surgical site infection, length of hospital stay, andtolerance of mechanical bowel preparation.Results: Participants in the Non-MBP group exhibited shorter times until first bowel movement (27.87 vs. 29.48 h), first passage of flatus (50.96 vs. 55.08 h), and first passage of stool (75.94 vs. 98.50 h);they also exhibited fewer postoperative gastrointestinal symptoms, including nausea (18.9% vs. 38.5%), vomiting (26.4% vs. 51.9%), abdominal pain (34.0% vs. 78.9%), and bloating (3.8% vs.26.9%). The plasma D-lactate and DAO were significantly increased (2.93 vs. 5.68 nmol/mL and 20.46 vs. 54.49 ng/mL) after bowel preparation in the MBP group; but no differences in the Non-MBP group after bowel preparation. Compared with the MBP group, surgical field visualisation was superior (92.45% vs. 78.85%), and the operation time was shorter (173.58 vs. 203.88 min) in the Non-MBP group. Patients undergoing MBP complained of bloating (82.35%), unpleasant taste (78.43%), sleep disturbance (70.59%), nausea (68.63%), abdominal pain (64.71%), vomiting(45.10%), polydipsia (33.33%), dizziness (25.49%), and headache (7.84%).Conclusions: Non-MBP is more conducive to the recovery of postoperative gastrointestinal function after surgery of gynaecological malignant tumour.Trial registration number: Chictr.org.cn.( ChiCTR2200059101).
The status of work-related coronavirus prevention measures and risk factors in hospitals
Authors: Dehghan Golnoush , Malekpour Fatemeh , Jafari-Koshki Tohid , Mohammadian Yousef , Rostami Hossein 
Published: August 2022

Abstract
Background: Hospitals around the world, counting in our nation, are confronting many problems and challenges within the battle against coronavirus infection. The aim of this study was to design a checklist and determining the status of preventive measures and risk factors that taken to combat coronavirus in the hospital.Methods: This cross-sectional and descriptive-analytical study was performed in Tabriz hospitals in Iran. A literature search was carried out in scientific databases. Based on the results of reviewing the texts and guidelines, a checklist was designed and validated to assess the status of occupational risk factors and prevention measures against coronavirus in hospitals. Results: Results of study confirmed validity of checklists for assessment of status of coronavirus prevention measures in hospitals. There was no significant difference in occupational risk factors of Covid-19 between governmental, non-governmental public, private and military hospitals. The risk of Total of preventive and control measures between governmental and military hospitals, Private and non-governmental public were not statistically significant.Conclusions: Hospital staff are more and more directly exposed to patients with Covid-19, the importance of preventive measures is doubled. This study can serve as a guide for occupational health professionals and other policymakers to improve the prevention and control of Covid-19 and even other infectious diseases in the future.
Behavioral and biochemical alterations in zebrafish model suggests AChE as a potential target for cytarabine neurotoxicological effects
Authors: charlie-silva Ives , Ferreira Violeta , Damasceno Évila Pinheiro , Teixeira Simone , Jimenez Paula , Carneiro Diana , Machado-Santelli Glaucia , Machado-Neto João , Olivier Danilo , Amaral Marcos , Soares Amadeu , Costa-Lotufo Leticia , Loureiro Susana , Pavlaki Maria 
Published: August 2022

Abstract
Due to their general cytotoxic effects, anticancer drugs pose a great risk to aquatic species, yet they have been among the least studied groups of drugs regarding their environmental impact. The present study aimed to identify the effects of the antileukemia drug cytarabine, also known as cytosine arabinoside (ara-C), on the development and neurobehavior of zebrafish larvae. Zebrafish eggs were exposed to cytarabine to assess survival, embryonic malformations, locomotor activity, DNA damage, and biochemical alterations. No mortality, teratogenicity, or genotoxicity was detected in zebrafish larvae up to 100 mg L-1. However, cytarabine decreased the total distance traveled by zebrafish under dark conditions, suggesting depression-like behavior in treated larvae. Furthermore, it was demonstrated that cytarabine exposure increased lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity, while it inhibited catalase (CAT) activity. Multivariate analysis suggested that both AChE and LPO correlated to behavioral changes, while molecular docking and dynamics simulations suggested a direct binding and stability of cytarabine to AChE. Further analyses of the effects of this anticancer compound in the cholinergic transmission are in progress to allow a better understanding of its neurotoxicity.
Molecular and Thermodynamic Insights into Interfacial Interactions Between Collagen and Cellulose Investigated by Molecular Dynamics Simulation and Umbrella Sampling
Authors: Ma Huaiqin , Shi Qingwen , Li Xuhua , Ren Junli , Li Zhijian , Ning Lulu 
Published: August 2022

Abstract
Cellulose/collagen composites have been widely used in biomedicine and tissue engineering. Interfacial interactions are crucial in determining the final properties of cellulose/collagen composite. Molecular dynamics simulations were carried out to gain insights into the interactions between cellulose and collagen. It has been found that the structure of collagen remained intact during adsorption. The results derived from umbrella sampling showed that (110) and (1-10) faces exhibited the strongest affinity with collagen, (100) face came the second and (010) the last, which could be attributed to the surface roughness and hydrogen-bonding linkers involved water molecules. Cellulose planes with flat surfaces and the capability to form hydrogen-bonding linkers produce stronger affinity with collagen. The occupancy of hydrogen bonds formed between cellulose and collagen was low and not significantly contributive to the binding affinity. These findings provided insights into the interactions between cellulose and collagen at the molecular level, which may guide the design and fabrication of cellulose/collagen composites.
Histological Assessment of Cortical Bone Changes in Diabetic Rats
Authors: Minami Masataka , Ikoma Kazuya , Onishi Okihiro , Horii Motoyuki , Itoh Kyoko , Takahashi Kenji 
Published: August 2022

Abstract
Background: Diabetes mellitus weakens bone strength due to the deterioration of bone quality; however, the histological mechanisms are still unknown. We hypothesized that histological assessment of cortical bone would enable us to determine the cause of the bone strength reduction associated with diabetes mellitus. Our aim was to evaluate the histomorphometric changes of cortical bone associated with deterioration of intrinsic bone properties and of bone quality in diabetes mellitus. Methods: We compared the outcomes of mechanical tests, bone mineral density measured using micro computed tomography, and histological assessments by applying Villanueva’s bone stain to the tibial bones of 40-week-old diabetic and control male rats. Results: With respect to mechanical testing, the maximum load and energy absorption were significantly lower in the diabetic group than in the control group, although fracture displacement and stiffness were not significantly different between the groups. Bone mineral density was significantly higher in the diabetic group than in the control group. Bone histomorphometry revealed that the diabetic rats had fewer osteocytes, greater cortical porosity, and increased mineralization in cortical bone compared with the control group. Conclusions: Increased mineralization of the cortical bone with greater cortical porosity leads to a weakening of bone strength in diabetes mellitus.
Macrophage polarity and wound age determination
Authors: Kuninaka Yumi , Ishida Yuko , Ishigami Akiko , Nosaka Mizuho , Matsuki Jumpei , Yasuda Haruki , Kofuna Ayumi , Kimura Akihiko , Furukawa Fukumi , Kondo Toshikazu 
Published: August 2022

Abstract
We investigated the dynamics of the gene expression of M1 and M2 macrophage markers during skin wound healing in mice. Expression of M1-macrophage markers, such as Il12a, Tnf, Il6, Il1b, and Nos2 was upregulated after wounding and peaked at 1 or 3 days after injury, and that of M2-macrophage markers such as Mrc1, Cd163, Ccl17, Arg, and Tgfb1, peaked at 6 days after injury. Consistent with these findings, using double-color immunofluorescence analysis revealed that CD80-positive M1 macrophages were more abundant than CD206-positive M2 macrophages on day 3 in mouse wound specimens, and that M2 macrophages were prominently detected in day 6 wounds. For application in forensic practice, we examined macrophage polarization using human wound specimens. The average ratios of HLA-DRα-positive M1 macrophages to CD163-positive M2 macrophages (M1/M2 ratios) were greater than 2.0 for the wounds aged 2–5 days. Furthermore, six of the eight samples individually exceeded the M1/M2 ratio of 2.0 in the range of 1.88–4.30. Therefore, an M1/M2 ratio significantly above 2.0 strongly indicates a wound age of 2–5 days. This study showed that M1 and M2 macrophages in human skin wound might be a promising marker for wound age determination.
Dissecting Novel Tumor Antigens and Immune Subtypes Guided mRNA Vaccine Development for HBV Related-Hepatocellular Carcinoma
Authors: Li Ang , Ye Bicheng , Lin Fangnan , Zhou Peiwen , Wang Yilin , Miao Xiaye , Jiang Yanfang 
Published: August 2022

Abstract
The mRNA vaccines are considered to be effective treatment strategies for cancers, but its progress in chronic hepatitis B virus (HBV) related-hepatocellular carcinoma (HCC) was slow. This study aimed to find potential antigens and identify suitable patients in HBV related-HCC for guiding mRNA vaccine development. We integrated the transcriptome RNA expression matrices and somatic mutation data from TCGA and ICGC datasets. A consistency matrix was constructed by using ConsensusClusterPlus to identify the immune subtypes. Graph learning based dimensional reduction was analyzed to establish immune landscape. Four upregulated and mutated antigens (EPS8L3, TCOF1, EZH2, and NOP56) were highly correlated with unfavorable clinical outcomes and antigen presenting cells (APCs). And two distinct immune phenotypes with differential clinical, cellular, and molecular characteristics were identified by in the ICGC and TCGA cohorts. IS1 is immune “hot” and immunosuppressive phenotype, with low tumor mutation burden (TMB) and high immune checkpoints (ICPs). On the contrary, IS2 is immune “cold” phenotype with high TMB and low ICPs. Monocle3 package was used to further study the intra-cluster heterogeneity, which identified cluster IS2A/2B within IS2 subtype was determined to be more suitable for mRNA vaccine. In summary, EPS8L3, TCOF1, EZH2, and NOP56 are potential antigens for mRNA vaccine development against HBV related-HCC, and patients in IS2A/2B are relatively more suitable for vaccination.
GhMYB18 confers Aphis gossypii Glover resistance through regulating the synthesis of salicylic acid and flavonoids in cotton plants
Authors: Zhang Jianmin , Hu Zongwei , Zhong Xiao , Zhang Haoran , Luo Xincheng , Wang Yuxue , Wang Yi , Liu Ting , Zhang Yi , Wang Xiangping , An Hongliu , Xu Dong , Wan Peng , Yang Yazhen 
Published: August 2022

Abstract

Accumulating researches confirmed that plant MYB transcription factors are involved in the defense response to herbivores. However, little is known about the target genes and signaling pathways that MYB transcription factors regulate. In this research, we identified the R2R3 MYB transcription factor GhMYB18 from upland cotton (Gossypium hirsutum L.). GhMYB18 is localized throughout the cell and is a functional transcription activation factor. The expression level of GhMYB18 was significantly up-regulated under aphid infestation and exogenous salicylic acid (SA) treatment. Overexpression of GhMYB18 in cotton cotyledons conferred enhanced tolerance of cotton plants to aphid (Aphis gossypii Glover) feeding, expectedly silencing GhMYB18 increased susceptibility of cotton plants to aphid. Subsequent analysis showed that GhMYB18 positively regulates defense-related enzyme activities including catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL), and increases the synthesis of salicylic acid and flavonoids to participate in the defense response against aphid. These results collectively suggest that the cotton MYB gene GhMYB18 is involved in the plant defense response to aphid attack, which may contribute to cotton aphid control strategies.
Emotion recognition based on group phase locking value using convolutional neural network
Authors: Cui Gaochao , Li Xueyuan , Touyama Hedeaki 
Published: August 2022

Abstract
Electroencephalography (EEG)-based emotion recognition is an important technology for human-computer interactions. In the field of neuromarketing, emotion recognition based on group EEG can be used to analyze the emotional states of multiple users. Previous emotion recognition experiments have been based on individual EEGs; therefore, it is difficult to use them for estimating the emotional states of multiple users. The purpose of this study is to find a data processing method that can improve the efficiency of emotion recognition.In this study, the DEAP dataset was used, which comprises EEG signals of 32 participants that were recorded as they watched 40 videos with different emotional themes. This study compared emotion recognition accuracy based on individual and group EEGs using the proposed convolutional neural network model. Based on this study, we can see that the differences of phase locking value (PLV) exist in different EEG frequency bands when subjects are in different emotional states. The results showed that an emotion recognition accuracy of up to 85\% can be obtained for group EEG data by using the proposed model. It means that using group EEG data can effectively improve the efficiency of emotion recognition. Moreover, the significant emotion recognition accuracy for multiple users achieved in this study can contribute to research on handling group human emotional states.
The effectiveness of HPV vaccination on the incidence of oropharyngeal cancers in males: A review
Authors: Macilwraith Philip , Malsem Eve , Dushyanthen Sathana 
Published: August 2022

Abstract
BackgroundHuman Papilloma Virus (HPV) contributes to the development of oropharyngeal cancer (OPC) and is currently the leading cause of OPC in the Western world. There have been limited studies examining the effect of HPV-vaccination on OPC incidence in males. This review aims to interrogate relationship linking HPV-vaccination and OPC in males, to potentially recommend pangender HPV-vaccination, to reduce the incidence of HPV associated OPC.Main BodyA review was carried out using Ovid Medline, Scopus and Embase databases, on 22nd October 2021 investigating the effect of HPV-vaccination on OPC prevalence in males and including studies with vaccination data pertaining to males in the past 5 years, while excluding those studies without appropriate oral HPV-positivity data and non-systematic reviews. Studies were evaluated as per the PRISMA guidelines and ranked using risk of bias tools including RoB-2, ROBINS-1 and the NIH quality assessment tools.7 studies were included ranging from original research to systematic review articles. All studies were published in English from 2017-2021. Overall, these suggested that HPV-vaccination reduced levels of oral HPV positivity in males. This was thought to be indicative of a reduced risk of development of HPV-associated OPC.A limitation of this study was the inability to conduct meta-analysis due to the heterogeneity of included studies. We noted a significant impact on the reduction of HPV positivity post HPV-vaccination and a potential contribution to reducing the future incidence of OPC.ConclusionThis review makes a strong case for pangender HPV-vaccination in combatting OPC in males.
Combined experiments and atomistic simulations for understanding the effect of ZnO on the microscopic network of sodium silicate and sodium borosilicate glasses
Authors: Ali Sk. Musharaf , Sahu Pooja , Shenoy K.T. , Arvind A. , Banerjee D. , Sugilal G. , Kaushik C.P. , Bhatt Kislay 
Published: August 2022

Abstract
Considering the beneficial aspects of ZnO doping in silicate and borosilicate glasses for bioactive glass, nuclear waste vitrified glass and many other applications, the combined experiments and molecular dynamics (MD) simulations were performed over wide range of composition. First principles ab-initio MD (AIMD) simulations confirmed the transferability of B-K-S potential model for multi-component glasses. A significant change in glass structure was monitored from short range order parameters: pair correlation function, coordination number, as well as intermediate range order parameters: bond/angle distribution profiles, structure factor and probability of X-O-X\ (X,X\ = Si, B, Zn) connections. Order of connectivity illustrated that hydrolysis of glass will slow down with addition of ZnO in the glass matrix. Successively, the effect of microscopic structure on observable glass properties: chemical durability, mechanical strength, thermal stability and characteristic vibrational spectra was analyzed. Results show good match of MD estimated trend for Young Modulus, glass transition temperature, and leaching data with the experimental observations, hereby, confirm the transferability of applied potential parameters for multi-component (n≥4) glasses. Both the experiments as well as MD simulations report the enhanced chemical durability of glass withZnO addition. Low R (Na2O/B2O3) and high K (SiO2/B2O3) of ZnO doped sodium borosilicate (Zn-NBS) glass surface compared to bare NBS represents the more stable structure of glass surface for Zn-NBS than NBS. During contact with water, Na+ ions were less likely to leach out from glass to aqueous solution for Zn doped glasses. The enhanced chemical resistivity of Zn-NBS was also established from the increasing activation energy for diffusion of Na ions. Likewise, the ring statistics as well as Na cluster size also supported the reduced chemical reactivity of Zn added glasses. The systematic study of linkage between network formers, ring statistics, diffusion dynamics and ion/water migration provides significant understanding of glass dissolution mechanism. Also, results predict the beneficial impact of ZnO doping for improved strength of glass skeleton, which would eventually control the glass degradation due to micro cracking. The combined studies from experiments and MD simulations disclose many interesting microstructure and dynamics due to the presence of ZnO in the glass.
Heterologous third and fourth dose vaccine to reduce severity and mortality in COVID-19 patients during delta and omicron predominance: A cohort study in Chiang Mai, Thailand
Authors: Intawong Kannikar , Chariyalertsak Suwat , Chalom Kittipan , Wonghirundecha Thanachol , Kowatcharakul Woravut , Ayood Pisittawoot , Thongprachum Aksara , Chotirosniramit Narain , Noppakun Kajohnsak , Teacharak Worachet , khammawan Pimpinan 
Published: August 2022

Abstract

Background
The Coronavirus disease 2019 (COVID-19) pandemic has evolved quickly, with numerous waves of different variants of concern resulting in the need for countries to offer continued protection through booster vaccinations. To ensure adequate coverage, Thailand has proactively adopted heterologous vaccination schedules. While studies have assessed homologous schedules in detail, the effectiveness of heterologous booster vaccine schedules against severity and mortality of COVID-19 patients, particularly with newer variants, remains to be explored fully.
Methods
Utilising an active Hospital Information System for COVID-19 established in Chiang Mai, Thailand, we conducted a cohort study by linking patient-level data on laboratory-confirmed COVID-19 cases to the national immunization records, during delta-predominant (1st October – 31st December 2021) and omicron predominant (1st February – 30th April 2022) periods. Demographic and baseline clinical characteristics associated with severe COVID-19 outcomes and mortality were examined for each period.
Results
COVID-19 cases during delta predominance were ten times more likely to have severe outcomes and in-hospital deaths as compared to omicron predominance. During omicron predominance, a third vaccine dose was associated with 89% reduced risk of both severe COVID-19 and deaths, as compared to the unvaccinated group. Those who received the third dose 14–90 days prior to the date of positive SARS-CoV-2 test had the highest protection against severe COVID-19 outcomes (93%) followed by a drop to 87% among those who received their last dose > 90 days prior. Severe outcomes were not observed among third dose recipients during delta predominance and fourth dose recipients during omicron predominance. All the vaccine types used for boosting in Thailand offered similar protection against severe COVID-19.
Conclusions
The risk of severe outcomes were significantly lower for COVID-19 patients with omicron as compared to delta. Booster doses provided very high level of protection against severe COVID-19 outcomes and deaths. Ongoing booster campaigns should focus on improving coverage utilising all available vaccines to ensure optimal protection.
Black and white: Molecular phylogeny reveals distinct evolutionary lineages of the Banded Krait, Bungarus fasciatus (Squamata: Elapidae) in Asia
Authors: Biakzuala Lal , Lalremsanga Hmar T. , Santra Vishal , Dhara Arindam , Ahmed Molla T. , Mallick Ziniya B. , Kuttalam Sourish , Malhotra Anita 
Published: August 2022

Abstract

The banded krait, Bungarus fasciatus is a widespread elapid snake, likely to comprise several distinct species in different geographic regions of Asia. Therefore, based on molecular phylogenetics and comparative morphology data, we present an overview of the systematic composition of the species to delimit potential biogeographic boundaries. Our phylogenetic analyses, based on four mitochondrial genes, reveal the existence of at least three evolutionary lineages within B. fasciatus, corresponding to Indo-Myanmar, Sundaic and eastern Asian lineages. We are convinced that there are at least three taxonomic entities within the nomen B. fasciatus, and we also designated a neotype in this study and restrict the distribution of B. fasciatus sensu stricto in the Indo-Myanmar region. We also provide additional natural history data of the taxon from eastern India. Finally, we advocate further studies to establish the degree of reproductive isolation among these diverging evolutionary lineages and to reassess the systematic status of this species complex especially the Sundaic and eastern Asian lineages.