bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of noble candidate gene associated with sensitivity to phytotoxicity of etofenprox in soybean
Authors: Kim Ji-Min , Ha Jungmin , Shin Ilseob , Lee Ju Seok , Park Jung-Ho , Lee Jeong-Dong , Kang Sungteag 
Published: September 2022

AbstractPhytotoxicity is caused by the interaction between plants and a chemical substance, which can cause critical damage to plants. Understanding the molecular mechanism underlying plant-chemical interactions is important for managing pests in crop fields and avoiding plant phytotoxicity by insecticides. The genomic region responsible for sensitivity to phytotoxicity of etofenprox (PE), controlled by a single dominant gene, was detected by constructing high density genetic map using recombination inbred lines (RILs) in soybean. The genomic region of ~ 80 kbp containing nine genes was identified on chromosome 16 using a high-throughput single nucleotide polymorphism (SNP) genotyping system using two different RIL populations. Through resequencing data of 31 genotypes, nonsynonymous SNPs were identified in Glyma.16g181900, Glyma.16g182200, and Glyma.16g182300. The genetic variation in Glyma.16g182200, encoding glycosylphosphatidylinositol-anchored protein (GPI-AP), caused a critical structure disruption on the active site of the protein. This structural variation of GPI-AP may change various properties of the ion channels which are the targets of pyrethroid insecticide including etofenprox. This is the first study that identifies the candidate gene and develops SNP markers associated with PE. This study would provide genomic information to understand the mechanism of phytotoxicity in soybean and functionally characterize the responsive gene.
A fine construction method of urban road DEM considering road morphological characteristics
Authors: Tao Yu , Tian Lei , Wang Chun , Dai Wen , Xu Yan 
Published: September 2022

AbstractUrban road DEM is not only an important basic geographic information data of the city, but also an important element to describe and express the urban topography, and it is an indispensable part of the construction of the smart digital city, urban planning and urban surface process simulation. Previous methods for constructing urban road DEMs do not sufficiently consider the actual morphological characteristics of urban roads, and morphological distortion is evident in the expression of urban roads, seriously affecting the application of urban rainfall flood simulation and urban pipe network design. In response to these problems, this study proposed a considering morphological characteristics fine (CMCF) method of urban road DEM construction, selected a typical urban road area in the Jianye District of Nanjing City in China as the study area, used the 1:500 digital line graphic as data source, hierarchized roads in accordance with different morphological characteristics from the perspective of DEM construction, constructed the corresponding DEMs, and finally merged all levels of road DEMs to produce a complete high-precision urban road DEM. Results showed that the DEM constructed using the CMCF method not only exhibited higher elevation accuracy than the urban road DEM constructed using previous methods, i.e., inverse distance weighting (IDW) and triangulated irregular network (TIN) methods, with a mean error and a root-mean-square error of 0.015 and 0.060, respectively, but it can also accurately express the spatial element composition form and road morphological characteristics of urban roads, avoiding the distorted expression of road morphological characteristics. This study can provide a reference for a new DEM construction method and data support for smart digital city construction and urban surface simulation.
Structural parameter variation working on the performance of anti-uplift multibell underreamed anchors
Authors: Chen Chen , Xia Yuanyou , Lin Manqing , Ni Qing 
Published: September 2022

AbstractThe structural parameters of multibell underreamed anchors play a crucial role in anchoring performance. The parameters of multibell underreamed anchor investigations are helpful when exploring optimized anchor structures. Based on the results of small-scale physical modelling tests, two types of multibell underreamed anchors were adopted under vertical uplifting loads. Numerical investigations were employed to study the effect of bell spacing, underream structure and bell dimension on the ultimate uplift bearing capacity. After an analysis of the anchorage mechanism, the anchoring efficiency was evaluated by the anchoring force provided by the unit concrete usage of the anchor, and the structural parameter λ equal to the surface area ratios of the expanded bell cone to the straight shaft between bells was defined. Then, the anchoring efficiency optimized structural parameters were presented. An analysis of model tests and simulation results showed that compared to concave bell surfaces, the convex shape could enhance the ultimate bearing capacity of a multibell underreamed anchor. There is an optimal value for the spacing of neighbouring bells, and there are three models of mechanisms for multibell anchors pulling out. When λ ∈ [1, 1.8], the multibell anchors can perform most efficiently to achieve their structural advantages.
Retro-2 alters Golgi structure
Authors: Yue Xihua , Gim Bopil , Zhu Lianhui , Tan Chuanting , Qian Yi , Graham Morven , Liu Xinran , Lee Intaek 
Published: September 2022

AbstractRetro-2 directly interacts with an ER exit site protein, Sec16A, inhibiting ER exit of a Golgi tSNARE, Syntaxin5, which results in rapid re-distribution of Syntaxin5 to the ER. Recently, it was shown that SARS-CoV-2 infection disrupts the Golgi apparatus within 6–12 h, while its replication was effectively inhibited by Retro-2 in cultured human lung cells. Yet, exactly how Retro-2 may influence ultrastructure of the Golgi apparatus have not been thoroughly investigated. In this study, we characterized the effect of Retro-2 treatment on ultrastructure of the Golgi apparatus using electron microscopy and EM tomography. Our initial results on protein secretion showed that Retro-2 treatment does not significantly influence secretion of either small or large cargos. Ultra-structural study of the Golgi, however, revealed rapid accumulation of COPI-like vesicular profiles in the perinuclear area and a partial disassembly of the Golgi stack under electron microscope within 3–5 h, suggesting altered Golgi organization in these cells. Retro-2 treatment in cells depleted of GRASP65/55, the two well-known Golgi structural proteins, induced complete and rapid disassembly of the Golgi into individual cisterna. Taken together, these results suggest that Retro-2 profoundly alters Golgi structure to a much greater extent than previously anticipated.
Wearable accelerometer-derived physical activity and incident disease
Authors: Khurshid Shaan , Weng Lu-Chen , Nauffal Victor , Pirruccello James P. , Venn Rachael A. , Al-Alusi Mostafa A. , Benjamin Emelia J. , Ellinor Patrick T. , Lubitz Steven A. 
Published: September 2022

AbstractPhysical activity is regarded as favorable to health but effects across the spectrum of human disease are poorly quantified. In contrast to self-reported measures, wearable accelerometers can provide more precise and reproducible activity quantification. Using wrist-worn accelerometry data from the UK Biobank prospective cohort study, we test associations between moderate-to-vigorous physical activity (MVPA) – both total MVPA minutes and whether MVPA is above a guideline-based threshold of ≥150 min/week—and incidence of 697 diseases using Cox proportional hazards models adjusted for age, sex, body mass index, smoking, Townsend Deprivation Index, educational attainment, diet quality, alcohol use, blood pressure, anti-hypertensive use. We correct for multiplicity at a false discovery rate of 1%. We perform analogous testing using self-reported MVPA. Among 96,244 adults wearing accelerometers for one week (age 62 ± 8 years), MVPA is associated with 373 (54%) tested diseases over a median 6.3 years of follow-up. Greater MVPA is overwhelmingly associated with lower disease risk (98% of associations) with hazard ratios (HRs) ranging 0.70–0.98 per 150 min increase in weekly MVPA, and associations spanning all 16 disease categories tested. Overall, associations with lower disease risk are enriched for cardiac (16%), digestive (14%), endocrine/metabolic (10%), and respiratory conditions (8%) (chi-square p < 0.01). Similar patterns are observed using the guideline-based threshold of ≥150 MVPA min/week. Some of the strongest associations with guideline-adherent activity include lower risks of incident heart failure (HR 0.65, 95% CI 0.55–0.77), type 2 diabetes (HR 0.64, 95% CI 0.58–0.71), cholelithiasis (HR 0.61, 95% CI 0.54–0.70), and chronic bronchitis (HR 0.42, 95% CI 0.33–0.54). When assessed within 456,374 individuals providing self-reported MVPA, effect sizes for guideline-adherent activity are substantially smaller (e.g., heart failure HR 0.84, 95% CI 0.80–0.88). Greater wearable device-based physical activity is robustly associated with lower disease incidence. Future studies are warranted to identify potential mechanisms linking physical activity and disease, and assess whether optimization of measured activity can reduce disease risk.
Functionally diverse microbial communities show resilience in response to a record-breaking rain event
Authors: Walker Jordan R , Woods Alaina C , Pierce Mary K , Steichen Jamie L , Quigg Antonietta , Kaiser Karl , Labonté Jessica M 
Published: September 2022

Abstract
Estuaries provide many ecosystem services and host a majority of the world’s population. Here, the response of microbial communities after a record-breaking flood event in a highly urbanized estuary was followed. Hurricane Harvey (hereafter Harvey) was a category 4 hurricane that made landfall on the Texas coast in 2017 and lashed the Houston area with 1.4–1.7 × 1010 m3 of rainfall, disrupting the natural gradients of nutrients and salinity. Here, we utilized metagenomics to analyze how Harvey altered the microbial community of Galveston Bay over five weeks following the storm. We hypothesized that the community would shift from a marine dominated community to that of a terrestrial and freshwater origin. We found that following the storm there were changes in the distribution of species with specific metabolic capacities, such as Cyanobacteria, enriched in oxygenic photosynthesis and nitrogen fixation genes, as well as Verrucomicrobia and Betaproteobacteria, with high prevalence of the SOX complex and anoxygenic photosynthesis genes. On the other hand, dominant members of the community with more diverse metabolic capabilities showed less fluctuations in their distribution. Our results highlight how massive precipitation disturbances can alter microbial communities and how the coalescence of diverse microorganisms creates a resilient community able to maintain ecosystem services even when the system is in an altered state.
Dual-mode nanophotonic upconversion oxygen sensors
Authors: Heydari Esmaeil , AmirAhmadi Javad , Ghazyani Nahid , Bai Gongxun , Zare-Behtash Hossein , MajlesAra MohammadHossein 
Published: September 2022

Development of a 980 nm-excitable oxygen sensor based on time-resolved phosphorescence spectroscopy by doping NaYF4:Yb3+,Tm3+ upconversion nanoparticles in the PtTFPP-PS oxygen-sensitive matrix.
Insights into the structure and function of the histidine kinase ComP from Bacillus amyloliquefaciens based on molecular modeling
Authors: Wang Lulu , Fan Ruochen , Li Zhuting , Wang Lina , Bai Xue , Bu Tingting , Dong Yuesheng , Xu Yongbin , Quan Chunshan 
Published: September 2022

Abstract
The ComPA two-component signal transduction system (TCS) is essential in Bacillus spp. However, the molecular mechanism of the histidine kinase ComP remains unclear. Here, we predicted the structure of ComP from Bacillus amyloliquefaciens Q-426 (BaComP) using an artificial intelligence approach, analyzed the structural characteristics based on the molecular docking results and compared homologous proteins, and then investigated the biochemical properties of BaComP. We obtained a truncated ComPS protein with high purity and correct folding in solution based on the predicted structures. The expression and purification of BaComP proteins suggested that the subdomains in the cytoplasmic region influenced the expression and stability of the recombinant proteins. ComPS is a bifunctional enzyme that exhibits the activity of both histidine kinase and phosphotransferase. We found that His571 played an obligatory role in the autophosphorylation of BaComP based on the analysis of the structures and mutagenesis studies. The molecular docking results suggested that the HATPase_c domain contained an ATP-binding pocket, and the ATP molecule was coordinated by eight conserved residues from the N, G1, and G2 boxes. Our study provides novel insight into the histidine kinase BaComP and its homologous proteins.
Gr1+ myeloid-derived suppressor cells participate in the regulation of lung–gut axis during mouse emphysema model
Authors: Yang Jing , Zeng Jiajia , Yang Shuaini , Guan Xin , Gao Qiaoying , He Simeng , Wu Xiaoyang , Ge Lixiu , Bai Hong 
Published: September 2022

Abstract
Background: Chronic obstructive pulmonary disease (COPD) is often accompanied by intestinal symptoms. Myeloid-derived suppressor cells (MDSCs) possess immunosuppressive ability in cancer, chronic inflammation, and infection. The aim of this study was to verify the distribution of MDSCs in emphysema mouse model and participation in lung–gut cross-talk.
Methods: Adult male C57BL/6 mice were exposed to cigarette smoke (CS) for 6 months or injected with porcine pancreas elastase to establish emphysema models. Flow cytometry and immunohistochemistry analysis revealed the distribution of MDSCs in tissues. The expression of inflammation and MDSCs-associated genes in the small intestine and colon were analyzed by real-time PCR.
Results: The small intestine and colon of CS-induced emphysematous mice displayed pathological changes, CD4+/CD8+ T cells imbalance, and increased neutrophils, monocytes, and macrophages infiltration. A significant expansion of MDSCs could be seen in CS-affected respiratory and gastrointestinal tract. Importantly, higher expression of MDSCs-related effector molecules inducible nitric oxide synthase (INOS), NADPH oxidase 2 (NOX2), and arginase 1 (ARG-1) suggested the immunosuppressive effect of migrated MDSCs (P&lt;0.05).
Conclusion: These data provide evidence for lung–gut axis in emphysema model and the participants of MDSCs.
Readability of Cochlear Implant Brochures: A Potential Factor in Parent Decision Making
Authors: La Scala Jennifer D. , Zraick Richard I. , Rosa-Lugo Linda I. , Cosby Janel L. 
Published: September 2022

Purpose:
The purpose of this study was to examine the ease of reading cochlear implant (CI) brochures provided to parents and caregivers who are making informed decisions about the management of their child's hearing loss.

Method:
CI brochures from three Food and Drug Administration–approved CI manufacturers were examined: Advanced Bionics, Cochlear Americas, and MED-EL. Reading grade levels and ease of reading were analyzed using a commercially available computer software program, applying six readability formulas commonly used to examine hearing-related patient education materials (PEMs).

Results:
The readability of the CI brochures exceeds the fifth- to sixth-grade reading-level guidelines. The CI brochures may be difficult for the average English-speaking adult to read with ease and requires at least a 10th-grade comparable reading level.

Conclusions:
Despite health literacy initiatives, audiology-focused PEMs continue to be created without full consideration of the burden for the reader. Authors of PEMs should consider the average reading level of the reader as a variable potentially influencing the decision-making process. Likewise, clinicians should consider the average reading level needed to understand PEMs when presenting information and resources to parents and caregivers for informed and shared decision making.
Measuring Communicative Congruence and Communicative Dysphoria in a Sample of Individuals Without Voice Disorders
Authors: Welch Brett , Helou Leah B. 
Published: September 2022

Purpose:

This study examines communicative congruence and communicative dysphoria in adults who deny having a voice disorder.
Communicative congruence
is the degree to which a person's communication is consistent with their sense of self/identity.
Communicative dysphoria
is the psychological entropy resulting from communicative
in
congruence. We propose that these experiences may influence patients' psychosocial well-being and are thus relevant to the field of speech-language pathology. We hypothesized that both constructs would be normally distributed with an inverse relationship. We also hypothesized that communicative congruence would predict scores on the Center for Epidemiological Studies–Depression (CES-D) scale, subscales of the Big Five Aspect Scales (BFAS; a personality measure), and achieve convergent validity with the Vocal Congruence Scale (VCS).

Method:
Participants (adults 18–70 years) completed the BFAS, CES-D, VCS, and the Voice Handicap Index–10 (VHI-10) before recording a series of speech tasks. Participants' recordings were played back while they responded to questions probing their communicative congruence and communicative dysphoria.

Results:

The 196 participants were predominantly female (67.3%) and cisgender (96.4%). Communicative congruence was negatively skewed, and communicative dysphoria was normally distributed. Both variables significantly related to each other: More
in
congruence was associated with more dysphoria. Communicative congruence was inversely related to CES-D scores. The personality metatrait Plasticity related to communicative congruence, as did the domain of Extraversion and the aspects Withdrawal, Enthusiasm, and Assertiveness. Communicative congruence achieved high convergent validity with the VCS.

Conclusions:

Counter to our hypothesis, participants reported more congruence than incongruence, but all other hypotheses were supported. Participants who reported
in
congruence were more likely to report symptoms of depression. These findings suggest that even in a predominantly cisgender cohort, some individuals' mental well-being might relate to how well their communication aligns with their identity. This work may inform future investigations into these constructs and their effects on voice therapy outcomes.

Supplemental Material:

https://doi.org/10.23641/asha.20739967
EDAfuse: A encoder–decoder with atrous spatial pyramid network for infrared and visible image fusion
Authors: Nie Cairen , Zhou Dongming , Nie Rencan 
Published: September 2022

AbstractInfrared and visible images come from different sensors, and they have their advantages and disadvantages. In order to make the fused images contain as much salience information as possible, a practical fusion method, termed EDAfuse, is proposed in this paper. In EDAfuse, the authors introduce an encoder–decoder with the atrous spatial pyramid network for infrared and visible image fusion. The authors use the encoding network which includes three convolutional neural network (CNN) layers to extract deep features from input images. Then the proposed atrous spatial pyramid model is utilized to get five different scale features. The same scale features from the two original images are fused by our fusion strategy with the attention model and information quantity model. Finally, the decoding network is utilized to reconstruct the fused image. In the training process, the authors introduce a loss function with saliency loss to improve the ability of the model for extracting salient features from original images. In the experiment process, the authors use the average values of seven metrics for 21 fused images to evaluate the proposed method and the other seven existing methods. The results show that our method has four best values and three second‐best values. The subjective assessment also demonstrates that the proposed method outperforms the state‐of‐the‐art fusion methods.
Kartografische Darstellung regionaler Unterschiede der
Infektionsraten beim Coronavirus (COVID-19): Ergebnisse einer bayesianischen
Glättung über Landkreise Bayerns
Authors: Loidl Verena , Koller Daniela , Mansmann Ulrich , Manz Kirsi Marjaana 
Published: September 2022

Zusammenfassung
Ziel der Studie Seit Beginn der aktuellen COVID-19 Pandemie sind
Übersichtskarten zur räumlichen Darstellung des
Infektionsgeschehens von großem öffentlichen Interesse. Aus
methodischer und risikokommunikativer Sicht sind diese Darstellungen nicht
unproblematisch, da zufällige Schwankungen oder Extremwerte auftreten
und tatsächliche regionale Verteilungsmuster überdeckt sein
können. Ein potentieller Lösungsansatz, um diesen
Herausforderungen zu begegnen, besteht in Glättungen durch Bayesianische
Verfahren. Ziel dieser Arbeit ist es, anhand von räumlich
geglätteten Übersichtskarten die Veränderungen der
Inzidenzverhältnisse im zeitlichen Verlauf in den Landkreisen und
kreisfreien Städten in Bayern zu untersuchen.
Methodik Die Daten zu SARS-CoV-2 wurden vom Bayerischen Landesamt
für Gesundheit und Lebensmittelsicherheit am 29.10.2021 und 17.02.2022
zur Verfügung gestellt. Die demographischen Daten pro Kreis sind dem
Statistischen Bericht des Bayerischen Landesamtes für Statistik
für das Jahr 2019 entnommen. Betrachtet wurden vier Altersgruppen pro
Geschlecht (<18, 18–29, 30–64,>64 Jahre)
aufgeteilt in 16 Zeiträume (28.01.2020 bis 31.12.2021). Die Karten
basieren auf standardisierten Inzidenzraten, die räumlich durch
Bayesianische hierarchische Modelle geglättet wurden.
Ergebnisse Das Standardisierte Inzidenzverhältnis (SIR) variierte
deutlich zwischen den Kreisen. Variationen traten für jeden Zeitraum
auf, wobei sich über den zeitlichen Verlauf sich ändernde
regionale Verteilungsmuster zeigten.
Schlussfolgerung Geglättete Gesundheitskarten eignen sich, um
Veränderungen der Inzidenzverhältnisse im zeitlichen Verlauf bei
COVID-19 in Bayern darzustellen und bieten gegenüber traditionellen
Karten den Vorteil, dass sie realitätsnähere Schätzungen
liefern, da sie Nachbarschaftsbeziehungen als erklärenden Faktor
einbeziehen. Der methodische Ansatz kann als erster Schritt gesehen werden, um
wichtige Erklärungsansätze für die beobachtete
Heterogenität zu identifizieren, und eine verbesserte
Risikokommunikation zu unterstützen.