bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA KCND1 protects hearts from hypertrophy
by targeting YBX1
Authors: Hou Yumeng , Li Liangliang , Yang Rui , Li Yingnan , Zhang Jing , Yang Na , zhang yuhan , Ji Weihang , Yu Tong , Lv Lifang , Lu Yanjie , Li Xuelian , li tianyu , Shan Hongli 
Published: July 2022

Abstract
Cardiac hypertrophy is a common structural remodeling in many cardiovascular diseases. Recently, long non-coding RNAs (lncRNAs) were found to be involved in the physiological and pathological processes of cardiac hypertrophy. In this study, we found that lncRNA KCND1 (LncKCND1) was downregulated in both transverse aortic constriction (TAC)-induced hypertrophic mouse hearts and Angiotensin II (Ang II)-induced neonatal mouse cardiomyocytes. Further analyses showed that knockdown of LncKCND1 impaired cardiac mitochondrial function and lead to hypertrophic changes in cardiomyocytes. In contrast, overexpression of LncKCND1 inhibited Ang II-induced cardiomyocyte hypertrophic changes. Importantly, enhanced expression of LncKCND1 protected the heart from TAC-induced pathological cardiac hypertrophy and improved heart function in TAC mice. Subsequent analyses involving mass spectrometry (MS) and RNA immunoprecipitation (RIP) assays showed that LncKCND1 directly binds to YBX1. Further, overexpression of LncKCND1 upregulated the expression level of YBX1 while silencing LncKCND1 had the opposite effect. Furthermore, YBX1 was downregulated during cardiac hypertrophy, whereas overexpression of YBX1 inhibited Ang II-induced cardiomyocyte hypertrophy. Moreover, silencing YBX1 reversed the effect of LncKCND1 on cardiomyocyte mitochondrial function and its protective role in cardiac hypertrophy, suggesting that YBX1 is a downstream target of LncKCND1 in regulating cardiac hypertrophy. In conclusion, our study provides mechanistic insights into the functioning of LncKCND1 and supports LncKCND1 as a potential therapeutic target for pathological cardiac hypertrophy.
Blood-brain-barrier dysfunction and folate and vitamin B12 levels in first-episode psychosis
Authors: Campana Mattia , Löhrs Lisa , Strauß Johanna , Münz Susanne , Oviedo-Salcedo Tatiana , Fernando Piyumi , Maurus Isabel , Raabe Florian , Moussiopoulou Joanna , Eichhorn Peter , Falkai Peter , Hasan Alkomiet , Wagner Elias 
Published: July 2022

Abstract
Background
Vitamin deficiency syndromes and blood-brain-barrier (BBB) dysfunction are frequent phenomena in psychiatric conditions. We analysed the largest available first-episode psychosis (FEP) cohort to date regarding routine cerebrospinal fluid (CSF) and blood parameters to investigate the association between vitamin deficiencies (vitamin B12 and folate) and BBB impairments in FEP.
Methods
We report a retrospective analysis of clinical data from all inpatients that were admitted to our tertiary care hospital with an ICD-10 diagnosis of a first-episode F2x (schizophrenia-spectrum) between January 1, 2008 and August 1, 2018 and underwent a lumbar puncture and blood-based vitamin status diagnostics within the clinical routine.
Results
222 FEP patients were included in our analyses. We report an increased CSF/serum albumin quotient (Qalb) as a sign of BBB dysfunction in 17.1% (38/222) of patients. White matter lesions (WML) were present in 29.3% of patients (62/212). 17.6% of patients (39/222) showed either decreased vitamin B12 levels or decreased folate levels. No statistically significant association was found between impairments in vitamin levels and an altered Qalb.
Conclusions
This retrospective analysis contributes to the discussion on the impact of vitamin deficiency syndromes in FEP. Although decreased vitamin B12 or folate levels were found in approximately 17% of our cohort, we found no evidence for significant associations between BBB dysfunction and vitamin deficiencies. To strengthen the evidence regarding the clinical implications of vitamin deficiencies in FEP, prospective studies with standardized measurements of vitamin levels together with follow-up measurements and assessment of symptom severity in addition to CSF diagnostics are needed.
Oropharyngeal Swallowing Physiology and Safety in Patients with Idiopathic Pulmonary Fibrosis: A consecutive descriptive case series
Authors: Alamer Mrs Amal , Jones Rhys , Drinnan Michael , Simpson Prof. A. John , Griffin Prof. Mike , Patterson Prof. Joanne M , Althuwaybi Abdullah , Ward Prof. Chris , Forrest Ian 
Published: July 2022

Abstract
Introduction:
Dysphagia occurs in multiple respiratory pathophysiologies, increasing the risk of pulmonary complications secondary to aspiration. Reflux associated aspiration and a dysregulated lung microbiome is implicated in Idiopathic Pulmonary Fibrosis (IPF), but swallowing dysfunction has not been described. We aimed to explore oropharyngeal swallowing in IPF patients, without known swallowing dysfunction.
Methods
Fourteen consecutive outpatients with a secure diagnosis of IPF were recruited and the 10-item Eating Assessment Tool (Eat 10) used to assess patient perception of swallowing difficulty. Oropharyngeal swallowing was assessed in ten patients using Videofluoroscopy Swallow Studies (VFSS). The studies were rated using validated scales: Penetration-Aspiration Scale (PAS); standardised Modified Barium Swallow Impairment Profile (MBSImP).
Results
EAT-10 scores indicated frank swallowing difficulty in 4/14 patients. Videofluoroscopy Studies showed that 3/10 patients had airway penetration, and one aspirated liquid without a cough response. Median MBSImp for oral impairment was 5, range [3–7] and pharyngeal impairment 4, range [1–14] indicating, overall mild alteration to swallowing physiology.
Conclusion
We conclude that people with IPF can show a range of swallowing dysfunction, including aspiration into an unprotected airway. To our knowledge, this is the first report on swallowing physiology and safety in IPF. We believe a proportion of this group may be at risk of aspiration. Further work is indicated to fully explore swallowing in this vulnerable group.
WITHDRAWN: Importance of lymph node metastasis to the pancreatic head region in patients with ampulla of Vater carcinoma
Authors: Tabuchi Motoyasu , Kimura Jiro , Sui Kenta , Iwata Jun , Noda Yoshihiro , Iiyama Tatsuo , Okabayashi Takehiro 
Published: July 2022

Abstract
Background: We analyzed clinicopathologic variables and postoperative outcomes in patients with ampulla of Vater carcinoma (AVC) to identify key predictors of post-resection prognosis.Methods: Fifty-five patients who underwent pancreaticoduodenectomy for AVC with curative intent between 2005 and 2020 were included in this study. A retrospective review of the clinical records of patients who underwent surgical exploration for AVC was performed using univariate and multivariate analyses. Clinical and pathological factors that influenced patient survival were analyzed. Results: One-, 3-, and 5-year overall survival rates after surgery were 97.4%, 71.8%, and 63.0%, respectively. A total of 21 patients developed recurrent disease after curative resection for AVC. The most frequently observed sites of recurrence were lymph nodes in 11 patients (52%), followed by the liver in 8 (38%), lung in 6 (29%), local in 3 (14%), and peritoneal dissemination in 3 (14%). Estimated 3- and 5-year recurrence free survival rates for the entire cohort were 62.8% and 57.2%, respectively. On multivariate analysis, only the presence of lymph node metastasis extending to the pancreatic head region (hazard ratio = 5.374; 95% confidence interval: 1.279–22.58; P = 0.022) predicted inferior relapse-free survival. A significant correlation between postoperative recurrence and pathological lymph node metastasis was observed. Conclusions: Lymph node metastasis, especially that which extends to the pancreatic head region, was clearly identified as a prognostic indicator of reduced relapse-free survival in patients who have undergone curative surgical treatment for AVC.
Evaluation of alpelisib-induced hyperglycemia prophylaxis and associated risk factors in PIK3CA-mutated hormone-receptor positive, human epidermal growth factor-2 negative advanced breast cancer
Authors: Burnette Sarah , Poehlein Emily , Lee Hui-Jie , Force Jeremy , Westbrook Kelly , Moore Heather N. 
Published: July 2022

Abstract
PurposeSOLAR-1 investigated alpelisib-fulvestrant (ALP+FLV) in patients with HR+/HER2-, PIK3CA-mutated advanced breast cancer and demonstrated a clinically significant increase in all-grade and grade (G) 3-4 hyperglycemia (HG) compared to placebo-fulvestrant. Given high rates of HG, a preventative protocol and identification of associated risk factors was implemented.MethodsThis single-center, retrospective study included patients receiving ALP+FLV. One week before ALP initiation, patients started an insulin-sensitizer. Patients had fasting plasma glucose (FPG) levels drawn day 8, 15, 28, then monthly. Primary outcome was incidence of G2-4 HG by day 28. Risk factors assessed included age, BMI, FPG, and hemoglobin A1c. Number of risk factors were compared between patients with and without HG.ResultsSixteen women were included with median age of 59 years. The cohort was 69% White, 25% Black, 75% with BMI > 25kg/m2, and 50% with history of diabetes. By day 28, 9 patients (56%) had G2-4 HG, with only 3 (19%) G3 and zero G4. Patients with G2-4 HG had a median of 2 risk factors compared to only 1 if no HG (p=0.03). 5 patients (31%) required a temporary hold of ALP and 3 (19%) required dose reduction due to HG. 13 patients permanently discontinued ALP – 9 due to disease progression and 4 from an adverse event (only 1 HG).ConclusionImplementation of a HG prophylaxis protocol with ALP in a single-center study demonstrated fewer G3-4 HG events compared to that seen in SOLAR-1 (19% vs 36.6%). An increase in HG-associated risk factors correlated with a higher incidence of G2-4 HG.
Prevalence of C-Shaped Canals in Maxillary and Mandibular Molars: A Retrospective Cone-Beam Computed Tomography study in an Iranian Population
Authors: Akheshteh vahid , Shokri Abbas , Akbarzadeh Mahdi , razaghi parnain 
Published: July 2022

Abstract
Background
This study evaluated the prevalence of first and second maxillary and mandibular molars with C-shaped canal separately according to gender, type and side of teeth and in terms of C-shaped classification in an Iranian sub-population
Methods
In this study, 3233 CBCT scans were obtained by Planmeca Scanner device by exposure time = 12 seconds, voxel size = 0.2mm, voltage of 80 kV and 15 mA. The images were observed by visualization software and the incidence of C-shaped canal was classified by Fan et al. (2004) methods.
Results
In this study the prevalence of C-shaped molars was equal to 4.86%. The incidence of the first and second C-shaped molars was also 2.81% and 5.47%; the incidence of the mandibular first and second C-shaped molars were 4.49% and 8.38%; and this prevalence in maxilla was also 1.47% and 2.32%. Significant differences existed regarding the incidence of C-shaped canal configurations in the first and second molars in total molars (p < 0.001) and mandibular molars (p < 0.004); however, no significant differences were seen regarding C-shaped canal configurations in the first and second maxillary molars .No significant difference was generally observed in the prevalence of C-shaped canals according to side of teeth and between males and females except in the maxilla. Among mandibular molars, C1 and C2 C-shaped configurations were seen more frequently.
Conclusion
Since the racial factors influence the anatomy of teeth, evaluating the prevalence of C-shaped canals in different populations to identify and raise the level of caution in the treatment of this type of root canals become more and more important.
Subacute Toxicity Analyses of Green Synthesized Magnetite Nanoparticles on Lemna minor L. (Duckweed) Plant
Authors: Filizler Buse , Haseki Selin , Ayisigi Melisa , Aktas Lale Yildiz 
Published: July 2022

Abstract
Magnetite nanoparticles (MNPs) are widely used in medicine, environmental technologies and biotechnology and green synthesis of MNPs could be an option to minimize potential environmental pollution by their usage. In this study, subacute toxicity of green synthesized magnetite (Fe3O4) nanoparticle was evaluated on Lemna minor, a main autotroph in lakes. Laurel (Laurus nobilis) leaf extract was used to synthesize the magnetite nanoparticles. Characterization of the nanoparticles were performed by UV/Vis spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Zeta size and potential and Scanning electron microscopy (SEM-EDS) analysis. Nanoparticles were around 108.5 nm, spherical in shape and capped with phyto-content. Subacute toxicity of magnetite nanoparticle was tested according to modified OECD 221 protocol, by treating L. minor plants with different MNPs concentrations (0.1, 1, 10, 100, 1000, 2000 mg L− 1) in petri dishes containing Steinberg medium for 15 days. The MNPs up to 1000 mg L− 1 did not cause any toxic effect on Lemna minor even it promoted growth and development of the plant in the concentrations less than 100 mg L− 1. The number of fronds, colonies and photosynthetic pigment contents significantly decreased by magnetite nanoparticle application of 1000 and 2000 mg L− 1 concentrations. Moreover, in these concentrations the nanoparticle caused oxidative stress indicated by the increased hydrogen peroxide and superoxide anion content and lipid peroxidation level. As a conclusion, this study showed that 1000 mg L− 1 green synthesized MNPs concentration is the starting point of subacute toxicity for L. minor.
LncRNA weighted gene co-expression network analysis reveals novel biomarkers related to prostate cancer metastasis
Authors: Liu Miao , Chen Man-Yun , Huang Jia-Meng , Liu Qian , Wang Lin , Liu Rong , Huang Wei-Hua , Zhang Wei , Yang Nian 
Published: July 2022

Abstract
Background: Most prostate cancer patients die from metastasis and lack accurate efficacious biomarkers to monitor the disease behavior, optimize treatment and assess prognosis. Herein, we aimed to identify meaningful lncRNA biomarkers associated with prostate cancer metastatic progression.Methods: By repurposing microarray probes, 11,624 lncRNAs in prostate cancer were obtained from Gene Expression Omnibus (GEO) database (GSE46691, N = 545; GSE29079, N = 235; GSE94767, N = 130). Weighted gene co-expression network analysis was applied to determine the co-expression lncRNA network pertinent to metastasis. Hub lncRNAs were screened. RNA-seq and clinical data from the Cancer Genome Atlas prostate cancer (TCGA-PRAD) cohort (N=531) were analyzed. Transwell assay and bioinformatic analysis were performed for mechanism research.Results: The high expression levels of nine hub lncRNAs (FTX, AC005261.1, NORAD, LINC01578, AC004542.2, ZFAS1, EBLN3P, THUMPD3-AS1, GAS5) were significantly associated with Gleason score and increased probability of metastatic progression. Among these lncRNAs, the Kaplan-Meier survival analyses showed higher expression of ZFAS1 was associated with shorter relapse free survival. In-vitro studies confirmed that downregulation of ZFAS1 decreased prostate cancer cell migration.Conclusion: We offered some new insights into discovering lncRNA markers correlated with metastatic progression of prostate cancer using the WGCNA. Some may serve as potential prognostic biomarkers and therapeutic targets for advanced metastatic prostate cancer.
Boosting the optical limiting properties of helicene-containing arene to the linear analogue
Authors: Xie Zheng , Yu Xiaohui , Li Yunfei , Wang Wei , Yuan Ziwei , Zhou Shuyun , Xiao Jinchong 
Published: July 2022

Abstract
Helicene-containing arene (5) and its linear analogue (8) have been successfully synthesized and characterized, where the single crystal X-ray diffraction analysis indicates that compound 5 can arrange in an offset packing style with a π-π overlap. More interestingly, the introduction of pentagon-rings into the parent skeletons in 5 and 8 can boost their stability, and molecule 5 possesses higher solubility in organic solvent than 8. Correspondingly, such structural difference has significantly influenced the optical limiting performance. Both of them exhibit reverse saturated absorption in solution and in doped gel glass towards 532 nm laser, and compound 5 presents higher optical limiting response than 8. This study can enrich the functionalization of helicene, which can possess a positive effect in terms of nonlinear optical property.
Investigation of filamentous fungi in wild Caatinga biome reptiles: microbiota research
Authors: Graça-Filho Renan Vasconcelos , Rocha Maria Gleiciane , Fonseca Xhaulla Maria Quariguasi Cunha , Teixeira Cínthia Firmo , Brandão Amaurício Lopes Rocha , Paiva Manoel Araújo Neto 
Published: July 2022

Abstract
Characterizing the fungal microbiota of free-living animals such as reptiles is necessary for a better understanding of fungal-host interactions as well as for establishing patterns of infections in these animals, thus creating an information network that helps understand the incidence and the microorganisms involved in this population for better maintenance and conservation of these species. Nonetheless, reports with this approach are limited, especially those focused on filamentous fungi. Therefore, this study aimed to isolate and identify filamentous fungi present in the oral cavity microbiota and cloaca of free-living reptiles of the Caatinga biome. Seven lizards, seven snakes, and two amphisbaenians were collected for mycological sampling, totaling 16 species of reptiles. The samples were collected from the oral and cloacal cavity of the animals, with sterile swabs, and sown in Sabouraud agar supplemented with chloramphenicol (0.05 g/L) and maintained for up to 14 days for the fungi isolation. Thus, 77 strains of filamentous fungi present in the microbiota of reptiles such as snakes, lizards, and amphisbaenian were isolated and identified. Among the most frequent fungi are the genera Aspergillus, Penicillium, and Microsporum, respectively. In short, knowledge of fungal diversity present in free-living reptiles of the Caatinga biome is of fundamental importance, since different opportunistic filamentous fungi have been isolated in this study, especially the genus Aspergillus, emphasizing the importance of monitoring these animals, as they can act as carriers and disseminators of potentially pathogenic agents.
Complex dynamics of a predator-prey model with hunting cooperation-induced fear and diffusion
Authors: Qi Haokun , Meng Xinzhu , Zhang Tonghua 
Published: July 2022

Abstract
Hunting cooperation is typical behavior among some large predators that increases the predator's ability to capture prey, and hunting cooperation can induce fear, which reduces the birth rate of prey. Based on this fact, in this paper we propose two predator-prey models with hunting cooperation-induced fear. In the first model, we consider the case without the spatial diffusion of populations: we study the bifurcation of the equilibrium points and their stability. Our study finds that (1) the model admits at most two positive equilibria, and when they exist, the model exhibits bistability structures: either between predator-free equilibrium and periodic solution, or between predator-free equilibrium and unique positive equilibrium. (2) When the model has a unique positive equilibrium, it undergoes the Hopf bifurcation. In addition, we analyze the stability and direction of Hopf bifurcated periodic solution, which shows that low-level fear and high-intensity hunting cooperation can cause Hopf bifurcation. (3) If the two positive equilibria coincide, saddle-node bifurcation and Bogdanov-Takens bifurcation of codimension two may occur. Moreover, if the predator population does not hunt cooperatively, the saddle-node bifurcation and Bogdanov-Takens bifurcation disappear. Our research findings generalize the existing results. For the model with spatial diffusion, we first pay attention to whether the model has Turing instability, which favors the formation of biodiversity. We find that when the ratio of the diffusion rate of prey to the diffusion rate of predator exceeds a certain critical value, Turing instability occurs. In addition, we present the existence of Hopf bifurcation and discuss the stability, direction, and period of spatially homogeneous and inhomogeneous periodic solutions. That shows that diffusion accelerates the occurrence of Hopf bifurcation, which destabilizes the population in advance and turns the number of the population into a periodic oscillating. At last, the theoretical analysis is illustrated by numerical simulations. The results show that populations can exhibit predator extinction, stable coexistence, or oscillatory coexistence under the influence of hunting cooperation, fear, or diffusion.Mathematics Subject Classification 92B05 · 92D25 · 35K57 · 37G15
Comparison the effects and side effects of Covid-19 vaccination in patients with inflammatory bowel disease (IBD); A systematic scoping review
Authors: Tabesh Elham , Soheilipour Maryam , Rezaeisadrabadi Mohammad , Zare-Farashbandi Elahe , Mousavi-Roknabadi Razieh Sadat 
Published: July 2022

Abstract
Covid-19 disease is a pandemic disease that maybe more severe and mortal in people with immunodeficiency, such as those with inflammatory bowel disease (IBD), who are taking immunosuppressive drugs. On the other hand, no definitive treatment has been identified for it and the best way to control it is wide spread vaccination. The aim of this study was to evaluate the benefits and side effects of different vaccines in patients with IBD. Three Electronic databases [Medline (accessed from PubMed), Scopus, Science Direct, and Cochrane] were searched systematically without time limit, using MESH terms and the related keywords in English language. Considering the titles and abstracts, unrelated studies were excluded. The full texts of the remained studies were evaluated by authors, independently. Then, the studies' findings were assessed and reported. Total of 212 articles were obtained within the first step of searching, and 147 ones remained after removing the duplications. Through the title and abstract screening, 127 were removed. Finally, after reading the full text of the remained articles, 15 ones included in data extraction. All included studied were research study, and most of them (12/15) had prospective design. Totally, 8/15 studies were performed in single-center settings. In 8/15 studies, patients with IBD were compared with a control group. The results were summarized the in two categories: 1) the effect of vaccination, and 2) side effects. The effect of vaccination were assessed in 13/15 studies. Side effects of Covid-19 vaccination in patients with IBD were reported in 7/15 studies. Patients with IBD can be advised that vaccination may have limited minor side effects, but it can protect them from the serious complications of Covid-19 disease and its resulting mortality with a high success rate. They should be also mentioned in booster doses.