bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compost preparation, chemical analyses and users’ perception in the utilization of water hyacinth, Ethiopia
Authors: Tibebe Dessie , Jembere Kehali , Kidie Addisu , Adugna Marelign , Alem Teferi , Teshome Gizachew 
Published: July 2022

AbstractLake Tana is the largest freshwater body in Ethiopia. Currently, the lake has been facing alarming environmental degradation and loss of biodiversity due to the invasion of water hyacinth. Although the weed is invasive, it can be converted into various benefits. Hence, this study was conducted in North Eastern Lake Tana, Sheha Gomengie Kebele. The main objective is compost preparation in terms of its drying periods, analyses, and user perception. Physicochemical and nutrient analyses were performed according to the standard procedures. Acid digestion was used for heavy metal analyses. From the result, the pH measurements ranged from 7.619 ± 0.195 to 7.719 ± 0.261, and the moisture content ranged from 38.712 ± 0.680 to 49.60 ± 9.06%. The mean electrical conductivity (EC) values of all treatments of matured compost ranged from 2.780 ± 0.542 to 3.51 ± 0.971 ds/m. The TN values of the matured compost ranged from 0.420 ± 0.379 to 0.754 ± 0.194 on a dry weight basis. The overall mean values of the C:N ratio for all the treatments were 11.60 which is within an acceptable range. A high amount of available P concentrations was observed in all compost treatments which ranged from 2.740 ± 0.190 to 2.940 ± 1.410 g/kg. Moreover, the concentrations of heavy metals in all treatments were below the permissible limit of different agencies and there was also no significant difference in the mean values of analysis of variance at (P < 0.05). Therefore, the prepared compost can be recommended for better agricultural purposes. Considering users’ understanding of compost preparation as an opportunity, converting WH into compost is promising in terms of its rich supply and the possibility of preparing in the dry season where labor is abundant. Therefore, it can be one way of sustainably reducing WH adverse effects on the Lakeshore.
Intratunical injection of rat-derived bone marrow mesenchymal stem cells prevents fibrosis and is associated with increased Smad7 expression in a rat model of Peyronie’s disease
Authors: Wang Wenting , Ding Weifang , Zhang Xuebao , Wu Shuang , Yu Tianxi , Cui Xin , Xie Yaqi , Yang Diandong , Lin Chunhua 
Published: July 2022

Abstract
Objective
Peyronie’s disease (PD) is a fibrotic disorder of the penis, but effective treatments are lacking. Here, we observed the effects of rat-derived bone marrow mesenchymal stem cells (BMSCs) injection in the active phase and chronic phase in a rat model of PD, and the possible mechanism was analysed with fibroblasts derived from rat penile tunica albuginea (TA).

Methods
Thirty-two male Sprague-Dawley rats were divided into four groups. In sham group, the rats were injected with 50 µL of vehicle. In the PD group, the rats were injected with 50 µg TGF-β1. In the PD + BMSCs early treatment group, the rats were injected with 50 µg TGF-β1 and injected with 1 × 106 BMSCs after 1 day. In the PD + BMSCs late treatment group, the rats were injected with 50 µg TGF-β1 and injected with 1 × 106 BMSCs after 28 days. Twenty-seven days after the last injection, the erectile function of the rats was measured, and then, penile fibrosis was analysed by histology and western blot. In vitro, fibroblasts derived from rat penile TA were used to identify a possible antifibrotic mechanism of BMSCs, and a Smad7 expression vector was used as a positive control. Fibroblasts were pretreated with the Smad7 expression vector or BMSCs for 48 h and then activated with 10 ng/mL TGF-β1 for 24 h. Cells viability was assessed, and Smad7, collagen 3, elastase-2B and osteopontin expression levels were analysed by immunofluorescence and western blot. Furthermore, fibroblasts were transfected with Smad7 siRNA or scramble control to observe whether the effects of BMSCs could be offset.

Results
Erectile function obviously improved, and fibrosis of penile TA was prevented after BMSCs treatment compared with that in the rats with PD. Furthermore, the effects of BMSCs treatment in the active phase were better than those in the chronic phase. After cocultured with BMSCs, cell viability was not affected, Smad7 expression was upregulated, and collagen 3, elastase-2B and osteopontin levels were decreased in the TGF-β1-treated fibroblasts. After transfection with Smad7 siRNA, the antifibrotic effects of BMSCs were offset.

Conclusions
The antifibrotic effects of BMSCs treatment in the active phase of the PD rat model were better than those in the chronic phase. A possible mechanism of BMSCs treatment was related to increased Smad7 expression, suggesting a possible effective and safe procedure for the treatment of PD.
Paper 2: Performing rapid reviews
Authors: King Valerie J. , Stevens Adrienne , Nussbaumer-Streit Barbara , Kamel Chris , Garritty Chantelle 
Published: July 2022

Abstract
Background
Health policy-makers must often make decisions in compressed time frames and with limited resources. Hence, rapid reviews have become a pragmatic alternative to comprehensive systematic reviews. However, it is important that rapid review methods remain rigorous to support good policy development and decisions. There is currently little evidence about which streamlined steps in a rapid review are less likely to introduce unacceptable levels of uncertainty while still producing a product that remains useful to policy-makers.

Methods
This paper summarizes current research describing commonly used methods and practices that are used to conduct rapid reviews and presents key considerations and options to guide methodological choices for a rapid review.

Results
The most important step for a rapid review is for an experienced research team to have early and ongoing engagement with the people who have requested the review. A clear research protocol, derived from a needs assessment conducted with the requester, serves to focus the review, defines the scope of the rapid review, and guides all subsequent steps. Common recommendations for rapid review methods include tailoring the literature search in terms of databases, dates, and languages. Researchers can consider using a staged search to locate high-quality systematic reviews and then subsequently published primary studies. The approaches used for study screening and selection, data extraction, and risk-of-bias assessment should be tailored to the topic, researcher experience, and available resources. Many rapid reviews use a single reviewer for study selection, risk-of-bias assessment, or data abstraction, sometimes with partial or full verification by a second reviewer. Rapid reviews usually use a descriptive synthesis method rather than quantitative meta-analysis. Use of brief report templates and standardized production methods helps to speed final report publication.

Conclusions
Researchers conducting rapid reviews need to make transparent methodological choices, informed by stakeholder input, to ensure that rapid reviews meet their intended purpose. Transparency is critical because it is unclear how or how much streamlined methods can bias the conclusions of reviews. There are not yet internationally accepted standards for conducting or reporting rapid reviews. Thus, this article proposes interim guidance for researchers who are increasingly employing these methods.
Paper 1: Demand-driven rapid reviews for health policy and systems decision-making: lessons from Lebanon, Ethiopia, and South Africa on researchers and policymakers’ experiences
Authors: Mijumbi-Deve Rhona M. , Kawooya Ismael , Kayongo Edward , Izizinga Rose , Mamuye Hadis , Amog Krystle , Langlois Etienne V. 
Published: July 2022

Abstract
Background
Rapid reviews have emerged as an approach to provide contextualized evidence in a timely and efficient manner. Three rapid review centers were established in Ethiopia, Lebanon, and South Africa through the Alliance for Health Policy and Systems Research, World Health Organization, to stimulate demand, engage policymakers, and produce rapid reviews to support health policy and systems decision-making. This study aimed to assess the experiences of researchers and policymakers engaged in producing and using rapid reviews for health systems strengthening and decisions towards universal health coverage (UHC).

Methods
Using a case study approach with qualitative research methods, experienced researchers conducted semi-structured interviews with respondents from each center (n = 16). The topics covered included the process and experience of establishing the centers, stimulating demand for rapid reviews, collaborating between researchers and policymakers, and disseminating and using rapid reviews for health policies and interventions and the potential for sustaining and institutionalizing the services. Data were analyzed using thematic analysis.

Results
Major themes interacted and contributed to shape the experiences of stakeholders of the rapid review centers, including the following: organizational structural arrangements of the centers, management of their processes as input factors, and the rapid reviews as the immediate policy-relevant outputs. The engagement process and the rapid review products contributed to a final theme of impact of the rapid review centers in relation to the uptake of evidence for policy and systems decision-making.

Conclusions
The experiences of policymakers and researchers of the rapid review centers determined the uptake of evidence. The findings of this study can inform policymakers, health system managers, and researchers on best practices for demanding, developing and using rapid reviews to support decision- and policymaking, and implementing the universal healthcare coverage agenda.
Approximate solutions for set optimization with an order cone that has nonempty quasirelative interiors
Authors: Zhao Pengxu , Xu Yihong , Huang Bin 
Published: July 2022

AbstractIn a real normed linear space, when the quasirelative interior is not empty, a class of order relation is introduced with Minkowski difference. Two classes of nonlinear functions are introduced, and their properties are discussed. A class of approximately efficient solutions and approximate weakly efficient solutions are introduced for set optimization. With nonlinear functions, optimality conditions are established for approximate solutions. Some examples are given to illustrate our main results.
Depression and its associated factors among prisoners in East Gojjam Zone prisons, Northwest Ethiopia: a multi-centered cross-sectional study
Authors: Tiruneh Tesfahun , Amha Haile , Sintayehu Bitew Mezinew , Tafere Yilkal 
Published: July 2022

Abstract
Background
Little is known about the prevalence and risk factors for depression in this vulnerable population around the world, including Ethiopia. Furthermore, information on the health of inmates is limited. The study sought to assess the prevalence and associated factors for depression among prisoners in the East Gojjam Zone of Northwest Ethiopia.

Methods
Institution-based cross-sectional study was conducted in East Gojjam Zone prisons. Data were gathered from 462 eligible prisoners who were chosen using a computer-generated simple random sampling technique. The patient health questionnaire nine was used to assess an individual's depression level. The information was entered into Epi-Data Version 4.2 and exported to STATA Version 14.1 for further analysis. Variables with a P < 0.05 in the multivariable binary logistics regression were considered significant.

Results
In this study the prevalence of depression among prisoners was 50.43% (95% CI 46–55%). Having work inside prison (AOR 0.6, CI 0.37–0.96), have no history of mental illness (AOR 0.37, 95% CI 0.16–0.85), had monthly income greater than 1500 birr (AOR 0.16, CI 0.05–0.5), Not thinking about the life after prison (AOR 0.4, 95% CI 0.27–0.64), and Prisoners who are sentenced for more than 5 years (AOR 2.2, CI 1.2–4), were significantly associated with depression.

Conclusions
According to this study, half of the prisoners in East Gojjam Zone prisons had depressive symptoms. Prisons should place a greater emphasis on the mental health of prisoners who have been sentenced for a long time, those who have a history of mental illness, and those who have no work in the prison.
Epigenetics may characterize asymptomatic COVID-19 infection
Authors: Arnold Cosby G. , Konigsberg Iain , Adams Jason Y. , Sharma Sunita , Aggarwal Neil , Hopkinson Andrew , Vest Alexis , Campbell Monica , Boorgula Meher , Yang Ivana , Gignoux Christopher , Barnes Kathleen C. , Monte Andrew A. 
Published: July 2022

AbstractRT-PCR is the foremost clinical test for diagnosis of COVID-19. Unfortunately, PCR-based testing has limitations and may not result in a positive test early in the course of infection before symptoms develop. Enveloped RNA viruses, such as coronaviruses, alter peripheral blood methylation and DNA methylation signatures may characterize asymptomatic versus symptomatic infection. We used Illumina’s Infinium MethylationEPIC BeadChip array to profile peripheral blood samples from 164 patients who tested positive for SARS-CoV-2 by RT-PCR, of whom 8 had no symptoms. Epigenome-wide association analysis identified 10 methylation sites associated with infection and a quantile–quantile plot showed little inflation. These preliminary results suggest that differences in methylation patterns may distinguish asymptomatic from symptomatic infection.
Estimating and comparing the duration of adolescent growth peak in skeletal class I and III subjects using cervical vertebral maturation method
Authors: Naderi Mohammad H. , Biria Mina , Shahbazi Soheil , Kousha Sina , Dalaie Kazem , Behnaz Mohammad 
Published: July 2022

Abstract
Background
Estimating skeletal maturation and growth potential is essential for developing adolescents' best orthodontic treatment plan. The purpose of this study was to compare the duration of adolescent growth peak in subjects of skeletal classes I and III using the cervical vertebral maturation (CVM) method.

Methods
This retrospective cross-sectional study included 116 Iranian subjects (skeletal class I = 68, skeletal class III = 48) aged 8–16 years old and without previous orthodontic treatments. Using Steiner and Wits analyses, two independent examiners traced pre-treatment lateral cephalograms to determine the subjects' skeletal relationship. The skeletal maturation was then assessed using Baccetti's CVM method. The onset and duration of adolescent growth peak (interval of CS3–CS4) were compared between two skeletal classes and two genders using independent samples t test.

Results
In skeletal class I and III subjects, the adolescent peak had a mean duration of 1.62 (± 1.33) and 2.00 (± 1.27) years, respectively. The average difference of 0.38 years (4.6 months) between the two groups was statistically significant (p < 0.001). Furthermore, the onset age of adolescent growth peak was 11.91 (± 1.32) and 12.08 (± 1.31) years old in class I and III subjects, respectively. This age difference was not statistically significant (p = 0.630). Males’ adolescent growth peak occurred 1.44 years later (p < 0.001) and lasted 0.20 years less (p < 0.001).

Conclusions
The adolescent growth peak started at a similar age in class I and III subjects, but the latter experienced the peak for 4.6 months longer. Moreover, females had an earlier and more extended adolescent growth peak.
Pharmacotherapy consideration of thrombolytic medications in COVID-19-associated ARDS
Authors: Amini Shahideh , Rezabakhsh Aysa , Hashemi Javad , Saghafi Fatemeh , Azizi Hossein , Sureda Antoni , Habtemariam Solomon , Khayat Kashani Hamid Reza , Hesari Zahra , Sahebnasagh Adeleh 
Published: July 2022

AbstractBackgroundIn late 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is responsible for coronavirus disease (COVID-19), was identified as the new pathogen to lead pneumonia in Wuhan, China, which has spread all over the world and developed into a pandemic. Despite the over 1 year of pandemic, due to the lack of an effective treatment plan, the morbidity and mortality of COVID-19 remains high. Efforts are underway to find the optimal management for this viral disease.Main bodySARS-CoV-2 could simultaneously affect multiple organs with variable degrees of severity, from mild to critical disease. Overproduction of pro-inflammatory mediators, exacerbated cellular and humoral immune responses, and coagulopathy such as Pulmonary Intravascular Coagulopathy (PIC) contributes to cell injuries. Considering the pathophysiology of the disease and multiple microthrombi developments in COVID-19, thrombolytic medications seem to play a role in the management of the disease. Beyond the anticoagulation, the exact role of thrombolytic medications in the management of patients with COVID-19-associated acute respiratory distress syndrome (ARDS) is not explicit. This review focuses on current progress in underlying mechanisms of COVID-19-associated pulmonary intravascular coagulopathy, the historical use of thrombolytic drugs in the management of ARDS, and pharmacotherapy considerations of thrombolytic therapy, their possible benefits, and pitfalls in COVID-19-associated ARDS.ConclusionsInhaled or intravenous administration of thrombolytics appears to be a salvage therapy for severe ARDS associated with COVID-19 by prompt attenuation of lung injury. Considering the pathogenesis of COVID-19-related ARDS and mechanism of action of thrombolytic agents, thrombolytics appear attractive options in stable patients without contraindications.
Anterior cruciate ligament microfatigue damage detected by collagen autofluorescence in situ
Authors: Kim Jinhee , Baek So Young , Schlecht Stephen H. , Beaulieu Mélanie L. , Bussau Lindsay , Chen Junjie , Ashton-Miller James A. , Wojtys Edward M. , Banaszak Holl Mark M. 
Published: July 2022

Abstract
Purpose
Certain types of repetitive sub-maximal knee loading cause microfatigue damage in the human anterior cruciate ligament (ACL) that can accumulate to produce macroscopic tissue failure. However, monitoring the progression of that ACL microfatigue damage as a function of loading cycles has not been reported. To explore the fatigue process, a confocal laser endomicroscope (CLEM) was employed to capture sub-micron resolution fluorescence images of the tissue in situ. The goal of this study was to quantify the in situ changes in ACL autofluorescence (AF) signal intensity and collagen microstructure as a function of the number of loading cycles.

Methods
Three paired and four single cadaveric knees were subjected to a repeated 4 times bodyweight landing maneuver known to strain the ACL. The paired knees were used to compare the development of ACL microfatigue damage on the loaded knee after 100 consecutive loading cycles, relative to the contralateral unloaded control knee, through second harmonic generation (SHG) and AF imaging using confocal microscopy (CM). The four single knees were used for monitoring progressive ACL microfatigue damage development by AF imaging using CLEM.

Results
The loaded knees from each pair exhibited a statistically significant increase in AF signal intensity and decrease in SHG signal intensity as compared to the contralateral control knees. Additionally, the anisotropy of the collagen fibers in the loaded knees increased as indicated by the reduced coherency coefficient. Two out of the four single knee ACLs failed during fatigue loading, and they exhibited an order of magnitude higher increase in autofluorescence intensity per loading cycle as compared to the intact knees. Of the three regions of the ACL - proximal, midsubstance and distal - the proximal region of ACL fibers exhibited the highest AF intensity change and anisotropy of fibers.

Conclusions
CLEM can capture changes in ACL AF and collagen microstructures in situ during and after microfatigue damage development. Results suggest a large increase in AF may occur in the final few cycles immediately prior to or at failure, representing a greater plastic deformation of the tissue. This reinforces the argument that existing microfatigue damage can accumulate to induce bulk mechanical failure in ACL injuries. The variation in fiber organization changes in the ACL regions with application of load is consistent with the known differences in loading distribution at the ACL femoral enthesis.
Needle arthroscopy in anatomical reconstruction of the lateral ankle: a report of three cases with a parallel comparison to the standard arthroscopy procedure
Authors: Lopes R. , Noailles T. , Padiolleau G. , Bouguennec N. , Vieira T. D. 
Published: July 2022

Abstract
Purpose
This study evaluates the use of the needle arthroscopy in anatomical reconstruction of the lateral ankle. We hypothesized that the needle arthroscopy would allow anatomical reconstruction to be performed under arthroscopy.

Methods
Three patients underwent treatment of chronic ankle instability. The comparative procedure was performed in the following four steps: 1) anteromedial articular exploration (medial/lateral gutter/anterior chamber/syndesmosis); 2)creation of the talar tunnel via the anteromedial arthroscopic approach; 3) anterolateral fibular tunneling; and 4) positioning of the graft by the anteromedial arthroscopic approach.
For each of these steps, the planned procedure using the needle arthroscope was compared to the standard arthroscope. For each step, the planned procedure using the needle arthroscopy was compared to the standard arthroscope and the act was classified based on level of difficulty: facilitated, similar, complicated and impossible.

Results
The exploration of the medial and lateral gutter, the creation of the tunnel of the talus and graft positioning were not accomplished using the needle arthroscope. While the syndesmosis visualization was facilitated by the needle arthroscope in comparison to the standard arthroscope.

Conclusion
The anatomical reconstruction of the lateral ankle, using the needle arthroscopy-only approach, was impossible in all three cases, regarding: ankle joint exploration, creation of the tunnel of the talus and graft positioning. The needle arthroscope should not be considered as a "mini arthroscope" but as a new tool with which it is necessary to rethink procedures to take advantage of the benefits of this instrument.
Return to sports after ACL injury 5 years from now: 10 things we must do
Authors: Gokeler Alli , Grassi Alberto , Hoogeslag Roy , van Houten Albert , Lehman Tim , Bolling Caroline , Buckthorpe Matthew , Norte Grant , Benjaminse Anne , Heuvelmans Pieter , Di Paolo Stefano , Tak Igor , Villa Francesco Della 
Published: July 2022

AbstractBackgroundThe outcome after ACL reconstruction (ACLR) is in general disappointing with unacceptable number of athletes that do not return to pre-injury level of sports, high re-injury rates, early development of osteoarthritis and shorter careers. Athletes after ACLR have high expectation to return to sports which is in contrast with the current outcomes. The aim of this manuscript is to present an overview of factors that are needed to be incorporated and to personalize the rehabilitation process for an athlete who has undergone an ACLR.Level of evidence4.