bluuebunny/crossref_metadata_2025_split huggingface dataset:
IMPLEMENTASI KEBIJAKAN PENGAWAS DALAM MENINGKATKAN KINERJA KEPALA MAN 2 TULUNGAGUNG
Authors: Yunis Hidayati  , Nik Haryanti  
Published: May 2022

Kepala sekolah merupakan salah satu elemen dasar bagi kemajuan sekolah. Kinerja kepala sekolah merupakan tanggung jawab pengawasan. Pengawasan adalah menentukan apa yang telah dicapai, mengevaluasi dan menerapkan tindakan korektif, jika perlu memastikan sesuai dengan rencanaStrategi yang dilakukan oleh pengawas dalam meningkatkan kinerja kepala sekolah yaitu pemantauan, pemeriksaan, penilaian dan perbaikan. Kinerja kepala harus ditingkatkan dan diprioritaskan. Kepala sekolah sebagai administrator dan manajer pendidikan memegang kedudukan yang sangat penting, karena merupakan faktor penentu keberhasilan sekolah dalam menjalankan peran dan fungsinya di masyarakat, ia juga turut menetukan keberadaan sekolah tersebut di tengah-tengah masyarakat dan kemampuan dalam merespon kebutuhan-kebutuhan dan harapan masayarakat. Metode penelitiannya adalah kualitatif, dengan teknik pengumpulan data yang digunakan wawancara, observasi dan dokumentasi. Hasil penelitiannya adalah 1) Rencana program kepengawasan dalam meningkatkan kinerja kepala MAN 2 Tulungagung adalah melakukan identifikasi masalah, menyususn program pengawasan, melaksanakan program kepengawasan, mengevaluasi dan menganalisis hasil pengawasan, serta melakukan pembinaan berdasarkan hasil evaluasi. 2) Pelaksanaan Kepengawasan dalam meningkatkan Kinerja Kepala MAN 2 Tulungagung adalah: (a) melakukan pembinaan terhadap guru, kepala madrasah dan tenaga kependidikan yang berada di wilayah binaannya, (b) melakukan penilaian terhadap kinerja guru, kinerja kepala madrasah dan kinerja seluruh staf sekolah, dan (c) melakukan evaluasi dan monitoring pelaksanaan program sekolah beserta pengembangannya. 3) Evaluasi dan Tindak Lanjut Hasil Pengawasan dalam meningkatkan Kinerja Kepala MAN 2 Tulungagung , beberapa langkah kegiatan pengawasan yang harus dijalankan oleh seorang pengawas adalah evaluasi dan tindak lanjut setelah dilakukan penilaian, pembinaan dan pemantauan, selanjutnya melakukan tindak lanjut pengawasan ini dilakukan berdasarkan pada hasil evaluasi secara komprehensif terhadap seluruh kegiatan pengawasan.
PENGARUH GAYA KEPEMIMPINAN, PENGAWASAN DAN DISIPLIN KERJA TERHADAP KINERJA PEGAWAI PADA KANTOR KEPENDUDUKAN DAN CATATAN SIPIL KABUPATEN MAJENE
Authors: Sri Indira Hartawati  
Published: May 2022

This study aims to determine the effect of leadership style on employee performance at the Majene Regency Population and Civil Registry Office, to determine the effect of supervision on employee performance at the Majene Regency Population and Civil Registry Office, to determine the effect of work discipline on employee performance at the Majene Regency Population and Civil Registry Office. and find out which variable is more dominant in influencing employee performance at the Office of Population and Civil Registry of Majene Regency.
This study uses a quantitative approach with data analysis techniques using SPSS. The results showed that 1) Leadership Style had an effect on Employee Performance at the Department of Population and Civil Registry of Majene Regency, 2) Supervision had an effect on Employee Performance at the Department of Population and Civil Registry of Majene Regency, 3) Work Discipline had no effect on Employee Performance at the Department of Population and Civil Registration. Civil Registry of Majene Regency and 4) Leadership Style variable has more influence on Employee Performance at the Department of Population and Civil Registry of Majene Regency.

Keywords: leadership, supervision, work discipline, employee performance
The personal values and study skills can be better predictors of college success than personality traits.
Authors: Chaudhry Nisha , Omelchenko Natalia , Roxby Joseph , Nolan Joe 
Published: May 2022

The Big Five Model of Personality has limited support in the field of neuroscience and challenged by low factor–criterion correlation (0.2-0.3, N=142). To overcome these limitations, we previously attempted to create an alternative model. For that model we choose the traits that were consistent with the modern ideas of brain organization and brain functioning. This model had five personality parameters 1) Fear tolerance, the ability to overcome fears and the resistance to developing them in the first place; 2) Impulse tolerance, the ability to resist immediate impulses to gratify desires or correct homeostatic imbalances; 3) Emotional tolerance, the ability to resist the effects of emotion in favor of rationality; 4) Traditional tendency, the measure of likelihood that either a traditional or novel option will hold more sway; and 5) Self-conception, the rewarding value we assign to projected outcomes. Here, we attempted to see if the new model can serve as a better predictor of behaviors, more specifically the college success. We developed a short questionnaire based on the traits proposed by the model (24 items total), asked anonymous participants attending freshman or senior classes to complete it, and evaluate the responses. We clearly recognized the piloting nature of the administrated questions and a potential impact on assessment of low inventory questionnaires, but it was immediately clear that the questions addressing personality traits had limited link to the seniority of the students. In contrast, responses of students of different seniority had been clearly dissociated every time when relevant to college success personal values and skills were evaluated (correlation 0.24-0.47, N=24, p<0.05). Although more research is needed, the findings indicate that personal values and study skills are much better predictors of college success than personality traits.
Alcohol induced alterations in protein- protein interactions of transcription factors associated with proliferation changes in fasd and alcoholism.
Authors: Gainer James , Anthony Bruce 
Published: May 2022

Excessive alcohol consumption, during pregnancy (Fetal Alcohol Spectrum Disorders, FASD) or in adult alcoholism, induces significant developmental and neurological alterations that effect neuronal development or brain plasticity respectively. It has been shown that alcohol exposure leads to alterations in neuronal stem cell proliferation, apoptosis and cell migration. Our interest was to begin delineation of the mechanism(s) associated with altered growth patterns of neuronal stem cells associated with FASD and alcoholism. Recent studies on protein expression demonstrate alcohol induced increases in expression of cell cycle proteins that effect the G1/S phase transition and likely DNA replication. These include the E2F1 family of transcription factors that regulate transcription of S-phase specific genes at the start of DNA replication. In addition, alcohol is shown to induce overexpression of the E2F1 dimerization partner(s) DP1 family and the retinoblastoma protein (RB), both of which demonstrate importance in regulation of transcription from E2F1 consensus sites. However, the mechanism of altered E2F1 activity is poorly understood. We examined E2F1 interactions with both DP1 and RB by Co-immunoprecipitation of E2F1 and western blotting. We used cortical neuronal stems cells from a rat in cell culture with a moderate (400mg/dl for 8 hr.) alcohol exposure. We examined protein interaction changes for both DP family isoforms as well as the RB protein.Our antibody capture of E2F facilitated all 8 isoforms. We suggest that alterations in RB binding to E2F1 alter transcription of S-phase specific genes and increases transcription of S-phase specific genes in a premature fashion.
Theoretical and computational studies of wall friction flame acceleration mechanism with linear variation of planar flame speed.
Authors: Demir Sinan , Sezer Hayri , Akkerman Vyacheslav 
Published: May 2022

Majority of theories, associated with the variety of flame acceleration scenarios, are based on the "geometrical formulation”: namely, the wrinkled to planar flame speeds ratio, Sw / SL , is evaluated as the scaled increase in the flame surface area, while the entire combustionchemistry is immersed inSL , which is assumed to be constant. However, in the practical reality,SL may experience spatial and temporal variations; at least, due to the associated pressure andtemperature distribution within a combustor, and their evolution during burning. In the present work, we initiate the systematic study of a much more intriguing situation – when SL - variations are externally imposed in a manner being a free functional of the formulation. This is relevant, in particular, to multi-phase combustion in dusty environment, with a non-uniform distribution of combustible and/or inert dust; as well as to the event of spatial variations of the equivalenceratio. First, linear variation of spatialSL - distribution is incorporated into the Bychkov theories offlame acceleration due to wall friction [Physical Review E 72 (2005) 046307] and solved analytically. Second, we implement linear variation of flame speed into computational platform. A backbone for the platform is a fully compressible, finite-volume Navier-Stokes code solving for the hydrodynamics and combustion equations in a homogenously and non-homogeneously - gaseous, laminar environment. It is investigated how linear variation of flame speed can affect the flame acceleration mechanism. Theory also validated by means of computational simulations. This work is supported by the Alpha Foundation for the Improvement of Mine Safety and Health as well as West Virginia University’s Program to Stimulate Competitive Research (PSCoR) and West Virginia University’s Senate Award for Research and Scholarship.
Colonization of mosquitoes by Francisella tularensis via a nectar reservoir.
Authors: Cusick Austin , Payne Jessica , Gaughenbaugh Anna , Horzempa Joseph , Seeber Roger , Kenney Adam 
Published: May 2022

Francisella tularensis is a highly infectious bacterium that causes the potentially fatal disease tularemia. In parts of Europe, specifically Scandinavian countries, mosquitoes are capable vectors of F. tularensis. Presumably, mosquitoes become colonized with F. tularensis following extraction of blood from an infected mammal. Mosquitoes feed intermittently on nectar between blood meals, allowing for the possibility of a colonized mosquito transmitting F. tularensis to flower nectar. Consequently, flower nectar may act as a natural reservoir of F. tularensis. We aimed to assess if nectar was a viable reservoir of F. tularensis, as well as the ability of mosquitoes to ingest and transmit F. tularensis from one nectar source to another. Therefore, we first sought to determine the viability of F. tularensis in nectar by inoculating nectar, in comparison to water and a bacterial growth medium (TSBc). Bacteria were capable of surviving in sucrose and TSBc over a long period of time relative to water. In addition, mosquitoes (Aedes aegypti) that fed on a nectar surrogate (30% sucrose solution) inoculated with bacteria became colonized with F. tularensis as was determined by plating insect homogenates at various time points. The presence of F. tularensis was confirmed by PCR using Francisella-specific primers on the viable bacteria recovered from the mosquitoes that fed from the inoculated sucrose. We are currently investigating whether F. tularensis can be re-deposited into sterile nectar or a nectar surrogate by colonized mosquitoes.
Synthesis of trans-Pterocarpans.
Authors: Horner James , Mason McKensie , Van Aardt Theunis , Serra Jon 
Published: May 2022

The objective of the research project is to synthetically produce trans-pterocarpans, a new but non- naturally occurring flavonoid. In order to achieve this, a synthetic method must be developed to produce the desired stereo outcome of the pterocarpan product. First, a Williamson ether synthesis is performed to protect the hydroxyl functional group of a benzaldehyde molecule, to be later combined via an aldol condensation with a protected acetophenone. The aldol products can then be converted to trans- pterocarpans by sequential cyclization of the 5-membered ring and the 6-membered ring using protection and deprotection procedures. At this point, the aldol condensation has been completed; however, the reaction procedure needs to be optimized in order to achieve better yields. The presence of the aldol product was verified via NMR, including H1, C13, DEPT, HETCOR, and COSY spectrums.
Examination of ku70/80 proteins in non-homologous end-joining repair of double-stranded dna induced by alcohol exposure in neuronal stem cells.
Authors: Dahlheim Nichalos , Anthony Bruce 
Published: May 2022

Fetal Alcohol Spectrum Disorders (FASD) demonstrates increased DNA damage and ultimately apoptosis in neural crest stem cells necessary for early development. In adult alcoholism, neuronal stem cells also demonstrate increased DNA damage from chronic and binge drinking.Although increases in cell death are a hallmarks of stem cell alcohol exposure, there is little understanding of the mechanism that induces DNA damage. Previous comet assay suggest both single and double stained DNA breaks. In this study, neuronal stem cells were treated with 400 mg/dl alcohol over an 8 hour period and examined for double stranded DNA breaks. KU70/80 proteins are part of the repair complex for double stained DNA breaks and bind to non-homologous ends at DNA strand breaks. We suggested that increases in DNA breaks from alcohol my work through a similar repair mechanism to recovery from DNA damage. Examination of this protein was achieved by immunocytochemistry using exposed stem cells. The results in KU expression show only slight increases in expression of either KU70 or KU80 after alcohol exposure. This suggests that KU70/80 protein expression and repair is not the only DNA repair mechanism associated with alcohol exposure and cell death.
A high throughput screen of a natural product library for the discovery of novel antibiotics with efficiacy against Klebsiella pneumoniae.
Authors: Chang Brandon , Schmitt Deanna , Horzempa Joseph 
Published: May 2022

Klebsiella pneumoniae is a gram negative bacterium in the enterobacteriaceae family. This organism is an opportunistic pathogen that has been associated with antibiotic resistance in recent years. The discovery of new antibiotics is critical to combat this rising trend in resistance. The National Center for Natural Products Research (NCNPR) has assembled a library of over 2500 extracts derived from plants, fungi, marine, and algae species with potential antibacterial properties. In partnership with NCNPR, we have developed a rapid and effective method for screening large quantities of organic compounds for antibacterial sensitivity against K. pneumoniae. Bacteria were inoculated into a microtiter plate containing liquid growth medium. A small volume of each compound was added to a corresponding well in the microtiter dish.After incubation at 37°C, bacteria growth was measured using a plate reader. Wells lacking treatment or containing antibiotics known to be effective at inhibiting K. pneumoniae were used to identify normal or inhibited growth levels respectively. At least four extracts exhibited potential for inhibiting K. pneumoniae: blanket flower, smallflower globemallow, Arizona walnut, and tree fern. Future investigations will involve the characterization and a detailed analysis of the antimicrobial efficacy of these compounds.
Moss diversity associated with several micro-habitats at high elevations in the northern West Virginia mountains.
Authors: Deal Robert , Henke Abigail 
Published: May 2022

Moss specimens were collected within microhabitats from three sites within the northern West Virginia Mountains: western edge of the Dolly Sods National Wilderness, Canaan Valley, and Canaan Mountain. The microhabitats that were evaluated included sandstone rocks in full sun and rocks that were shaded; sunny and shaded sphagnum bogs and swamps; sun exposed and shaded soil; and shaded logs. Mosses from the microhabitats were collected, identified to species, and voucher specimens were prepared for storage at Shawnee State University. The overall goal of this research is to determine which mosses are associated with the several microhabitats typical of the high elevations of the northern West Virginia mountains. To date, 46 moss species in 27 Families have been identified from the eight microhabitats investigated.
Putative Sensory Structures Associated with the Food Canal of Hybomitra difficilis (Diptera: Tabanidae).
Authors: Setser Emily , Joy James 
Published: May 2022

The feeding tube of Hybomitra difficilis is made up of a short distal vestibule followed by a food canal that leads to the cibarium; the two regions demarcated by the vestibule/food canal junction. Two pairs of sensilla were consistently observed in the vestibular walls, the first pair of basiconic design in the mid-vestibular region, and the latter pair of setiform design at the base of the vestibule. Food canal sensilla, which varied from 45 to 73 (n = 20; mean = 50.15; ± 1 SD= 10.02), were aggregated in the distal and distal median regions of the food canal, with relatively few sensilla observed in the proximal food canal region. This aggregation was significant (X2 = 261.05; P < 0.0001), leading to rejection of the null hypothesis that sensilla were evenly distributed throughout the length of the food canal. Food canal sensilla were of the setiform or trichite type, with the exception of a single basiconic sensillum in each wall of the food canal of every fly examined. All but one basiconic sensilla were located in the distal-most region of the food canal. While basiconic sensilla varied in position (i.e., distance) from the vestibule/food canal junction, they were significantly aggregated (X 2 = 12.38; P < 0.0062) in the two median sections of the distal canal region, thus leading to the rejection of Ho that basiconic sensilla were evenly distributed in subdivisions (i.e., sections) of the distal food canal region.
Validation of Marker Genes for the Rapid Alkalinization Factor Pathway in Arabidopsis thaliana
Authors: Hoffmaster Marshall , Gilkerson Jonathan 
Published: May 2022

Most studies involving plant hormones has revolved around the study of small molecule hormones. Although signaling mechanisms for these are well understood, signaling mechanisms involving peptide hormones remain understudied. Our studies center on Rapid Alkalinization Factor (RALF), that when applied exogenously to plants causes rapid alkalization of the cell’s apoplast which inhibits cell growth and elongation. RALF consensus sequences have been found in all plant lineages examined, suggesting RALF plays a fundamental role in plant biology. The mechanisms by which RALF mediates its effects remain to be elucidated. We hypothesized, that like other plant signaling molecules, RALF influences plant growth in part by effecting transcriptional changes. This study aims to define the AtRALF1 transcriptional response in Arabidopsis thaliana and to establish bonafide RALF marker genes for molecular characterization of the pathway. Two microarray time-course experiments were performed, one with exogenously applied AtRALF1 and another with estrogen-inducible AtRALF1 transgenic lines. Over a 24-hour time-course with exogenous peptide and estrogen-induction, 4799 and 1128 genes significantly changed expression (p-value < 0.05, with a log2 fold difference of ± 1), respectively. We performed cluster and gene ontology analysis on those genes to elucidate what biological processes RALF influences and determined calcium signaling, membrane modifications, and defense responses to be significant. Comparing the time-courses for overlap identified a set of genes we think is the early transcriptional response for this pathway. We are currently validating targets by qPCR.
Next generation sequencing analysis and map-based cloning of RALF-insensitive Arabidopsis thaliana lines
Authors: Quigley Ryan , Gilkerson Jonathan 
Published: May 2022

The Rapid Alkalinization Factor (RALF) family, which plays a crucial role in plant growth and development, is a group of related peptide hormones found across the plant kingdom. RALF induces the alkalinization of the apoplast which triggers a signaling cascade that inhibits cell elongation and growth, although the full mechanism of action is unknown. In this project, a forward-genetics screen was used to identify EMS-mutagenized lines of Arabidopsis thaliana (Ler-0) that were resistant to AtRALF1-induced root growth arrest. 21 of these RALF-resistant mutant (RRM) lines were then re-sequenced using Illumina next-generation sequencing technologies in order to find causative mutations and potential mapping polymorphisms. The line RRM30 was found to have over 460 exonic SNPs after its genome was mapped to the reference and analyzed with the programs Geneious, Bowtie2, and SAMtools mpileup. These SNPs were then used to create a set of dCAPS mapping-markers for a bulked-segregant analysis of RRM30. Many of these markers have been tested and confirmed to be unique to RRM30 using PCR genotyping experiments and gel electrophoresis. Future examination of the bulked-segregant analysis should narrow down the causative mutation in this line and help identify genes required for a RALF response. The causative mutation will be confirmed with an allelism test to knockout lines of A. thaliana in this gene. Identification of genes required for this response will shed more light on the mechanism growth inhibition by RALF.
Predicting temperature at scales relevant for tree growth along elevational gradients in the central Appalachians
Authors: Jalil Amaris , Hoffman Sierra , Lesser Mark 
Published: May 2022

It is well established that tree species grow within a realized niche. However, within that niche tree performance may not be equal. Therefore, we sought to quantify growth differentiation along elevational and climatic gradients in central Appalachia. A major issue of modelling tree growth is that regional weather stations are unable to capture fine-scale variation in temperature patterns along ecological gradients. To account for this variation, a linear regression model of temperature as a function of elevation was developed using daily temperature and precipitation data obtained from the National Oceanic and Atmospheric Administration for nine different weather stations in and around Shenandoah National Park, from which fine-scale temperature patterns of specific stations could be plotted and predicted. The elevational range of our study spanned 294-1022 meters, with an average lapse rate of -0.007 °C/m for maximum temperature, and -0.005 °C/m for minimum temperature. Models of red oak ring width against climate and topographic variables found that predominantly spring and autumn maximum and minimum temperature, and precipitation from both the current and previous year, most strongly influenced growth performance. It was also discovered that while spring maximum temperatures have been increasing at higher elevations, they remain relatively stable at lower elevations. Conversely, spring minimum temperatures are increasing at lower elevations and not at high elevations, indicating that the temperature envelope is expanding at high elevations and constricting at lower elevations. This may have significant implications for how tree species will respond to changing climate differentially along ecological gradients.