bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of flood‐prone areas with GeoFlood: Lessons learned from the Tiber River case study
Authors: D'Angelo Claudia , Passalacqua Paola , Fiori Aldo , Volpi Elena 
Published: February 2022

AbstractFlood mapping is a vital component for sustainable land use in flood‐prone areas. Due to the frequency of flood events, local authorities demand effective yet simple methods for the preliminary identification of flood‐prone areas at large scales to subsequently define mitigation strategies. We focus here on the workflow GeoFlood, a parsimonious model which uses only high‐resolution Digital Terrain Models (DTMs) to define the geomorphological and hydrological information necessary for flood inundation mapping, thus allowing for large‐scale simulations at a reasonable computational cost. The purpose of the present study is to investigate the conditions under which GeoFlood is able to correctly reproduce inundation scenarios (with an assigned return period) and their flooding characteristics. Specifically, we analyze its performance over a highly urbanized area, the mid‐lower portion of the Tiber River (Italy). We simulated the 200‐year return period scenario and compared the results to those provided by the local authority. A sensitivity analysis is performed to quantify the influence of the main geometric and hydraulic parameters involved. Results show that GeoFlood produces rapid flood estimation than can be used in support of standard costly methods over large scales, quickly pointing out the critical flood‐prone areas, with consideration of all the uncertainties involved.
Heterotopic cervical pregnancy: Case report and literature review
Authors: Fan Yazhen , Du Aijun , Zhang Yinfeng , Xiao Nan , Zhang Yunshan , Ma Junfang , Meng Wenjia , Luo Haining 
Published: February 2022

AbstractCervical pregnancy (CP) is a rare form of ectopic pregnancy (EP) in which the embryo implants and grows inside the endocervical canal. Heterotopic cervical pregnancy is an even rare form of EP, in which at least two embryos are simultaneously implanted in different sites and only one in the uterine cavity. Although many treatment approaches are available, the ideal management remains unclear. Here, we describe two cases of CP caused by assisted reproductive technologies (ART). One case underwent fertilization with intracytoplasmic sperm injection (ICSI) for male factor infertility, and the other was frozen–thawed embryo transfer (FET) following conventional in vitro fertilization (IVF). Both cases were successfully treated with ultrasound‐guided cervical pregnancy aspiration, and intrauterine pregnancies were effectively protected. To the best of our knowledge, these two were rare case reports use aspiration without additional methods and intrauterine pregnancy achieved live birth.
A scoping review of autoantibodies as biomarkers for canine autoimmune disease
Authors: Treeful Amy E. , Coffey Emily L. , Friedenberg Steven G. 
Published: February 2022

AbstractBackgroundAutoantibody biomarkers are valuable tools used to diagnose and manage autoimmune diseases in dogs. However, prior publications have raised concerns over a lack of standardization and sufficient validation for the use of biomarkers in veterinary medicine.ObjectivesSystematically compile primary research on autoantibody biomarkers for autoimmune disease in dogs, summarize their methodological features, and evaluate their quality; synthesize data supporting their use into a resource for veterinarians and researchers.AnimalsNot used.MethodsFive indices were searched to identify studies for evaluation: PubMed, CAB Abstracts, Web of Science, Agricola, and SCOPUS. Two independent reviewers (AET and ELC) screened titles and abstracts for exclusion criteria followed by full‐text review of remaining articles. Relevant studies were classified based on study objectives (biomarker, epitope, technique). Data on study characteristics and outcomes were synthesized in independent data tables for each classification.ResultsNinety‐two studies qualified for final analysis (n = 49 biomarker, n = 9 epitope, and n = 34 technique studies). A high degree of heterogeneity in study characteristics and outcomes reporting was observed. Opportunities to strengthen future studies could include: (1) routine use of negative controls, (2) power analyses to inform sample sizes, (3) statistical analyses when appropriate, and (4) multiple detection techniques to confirm results.ConclusionsThese findings provide a resource that will allow veterinary clinicians to efficiently evaluate the evidence supporting the use of autoantibody biomarkers, along with the varied methodological approaches used in their development.
Molecular landscape of IDH‐wild‐type, H3‐wild‐type glioblastomas of adolescents and young adults
Authors: Shi Zhi‐Feng , Li Kay Ka‐Wai , Huang Queenie Jun‐Qi , Wang Wei‐Wei , Kwan Johnny Sheung‐Him , Chen Hong , Liu Xiang‐Zhi , Li Wen‐Cai , Chan Danny Tat‐Ming , Zhang Zhen‐Yu , Mao Ying , Ng Ho‐Keung 
Published: February 2022

AbstractObjectiveWe aimed to characterise glioblastomas of adolescents and young adults (AYAs) that were isocitrate dehydrogenase (IDH) wild type (wt) and H3wt.Materials and MethodsFifty such patients (aged 16–32) were studied by methylation profiling, targeted sequencing and targeted RNA‐seq.ResultsTumours predominantly clustered into three methylation classes according to the terminology of Capper et al. (2018): (anaplastic) pleomorphic xanthoastrocytoma (PXA) (21 cases), GBM_midline (15 cases) and glioblastoma RTK/mesenchymal (seven cases). Two cases clustered with ANA_PA, four cases with LGG classes and one with GBM_MYCN. Only fifteen cases reached a calibrated score >0.84 when the cases were uploaded to DKFZ Classifier. GBM_midline‐clustered tumours had a poorer overall survival (OS) compared with the PXA‐clustered tumours (p = 0.030). LGG‐clustered cases had a significantly better survival than GBM_midline‐clustered tumours and glioblastoma RTK/mesenchymal‐clustered tumours. Only 13/21 (62%) of PXA‐clustered cases were BRAF V600E mutated. Most GBM_midline‐clustered cases were not located in the midline. GBM_midline‐clustered cases were characterised by PDGFRA amplification/mutation (73.3%), mutations of mismatch repair genes (40.0%), and all showed H3K27me3 and EZH1P loss, and an unmethylated MGMT promoter. Across the whole cohort, MGMT promoter methylation and wt TERT promoter were favourable prognosticators. Mismatch repair gene mutations were poor prognosticators and together with methylation class and MGMT methylation, maintained their significance in multivariate analyses. BRAF mutation was a good prognosticator in the PXA‐clustered tumours.ConclusionMethylation profiling is a useful tool in the diagnosis and prognostication of AYA glioblastomas, and the methylation classes have distinct molecular characteristics. The usual molecular diagnostic criteria for adult IDHwt glioblastoma should be applied with caution within the AYA age group.
Social relationship influence on self‐care and health among older people living with long term conditions: A mixed‐methods study
Authors: LeBlanc Raeann G. , Chiodo Lisa , Jacelon Cynthia S. 
Published: February 2022

AbstractBackgroundSocial relationships influence health, yet less is known on how social relationships influence self‐care among older people living with multiple long‐term conditions.ObjectivesThe purpose of this study was to determine how social networks influence therapeutic self‐care behaviours and health among community dwelling older people living with multiple long‐term conditions.DesignExplanatory sequential mixed‐methods.Setting/SampleCommunity dwelling older people living in the Northeast United States.MethodsA cross‐sectional sample of eighty‐nine people aged sixty‐five and older participated in telephone surveys. A nested sample of twelve participants completed a follow‐up open‐ended interview. Descriptive statistics, bivariate correlations and regression statistics were used to examine the associations between social network features and functions with the dependent variables of therapeutic self‐care behaviours and health. Qualities that emphasised the contexts of the relationships were integrated in the data analysis.ResultsThe strength of the social network and level of social support influenced the outcomes of therapeutic self‐care and mental health. Thematic analysis expanded this explanation of self‐care in relation to social network size, psycho‐social support, activation of support, interaction frequency and type.ConclusionIn supporting older people living with multiple long‐term conditions, this nursing research offers new ways to understand close social relationships influence on self‐care in developing systems of care at the community level.Implications for practiceDesigning care systems that include small trusted formal and informal caregivers that integrate social network members could improve health outcomes and improve access to supportive resources.
The association between environmental greenness and the risk of food allergy: A population‐based study in Melbourne, Australia
Authors: Peters Rachel L. , Sutherland David , Dharmage Shyamali C. , Lowe Adrian J. , Perrett Kirsten P. , Tang Mimi L. K. , Lycett Kate , Knibbs Luke D. , Koplin Jennifer J. , Mavoa Suzanne 
Published: February 2022

AbstractBackgroundWhile exposure to environmental greenness in childhood has shown mixed associations with the development of allergic disease, the relationship with food allergy has not been explored. We investigated the association between exposure to environmental greenness and challenge‐confirmed food allergy in a large population‐based cohort.MethodsThe HealthNuts study recruited 5276 12‐month‐old infants in Melbourne, Australia, who underwent skin prick testing to peanut, egg, and sesame; infants with a detectable wheal underwent food challenges to determine food allergy status. Environmental greenness was estimated using the normalized difference vegetation index (NDVI) for five buffer zones around the infant's home address: at the home, 100 m, 500 m, 800 m, and 1600 m radial distances. Environmental greenness was categorized into 3 tertiles and mixed effects logistic regression models quantified the association between greenness and the risk of food allergy, adjusting for confounding and accounting for clustering at the neighborhood level.ResultsNDVI data were available for n = 5097. For most buffer zones, medium and high greenness, compared to low greenness, was associated with an increased risk of peanut allergy (eg, 100 m tertile 2 aOR 1.89 95% CI 1.22–2.95, tertile 3 aOR 1.78 95% CI 1.13–2.82). For egg allergy, the effect sizes were smaller (100 m tertile 2 aOR 1.52 95% CI 1.16–1.97, tertile 3 aOR 1.38 95% CI 1.05–1.82). Socioeconomic status (SES) modified the association between greenness and peanut allergy, but not egg allergy; associations were apparent in the low SES group but not in the high SES group (p for interaction 0.08 at 100 m). Air pollution (PM2.5) also modified the associations between environmental greenness and food allergy, with associations present in high air pollution areas but not low (p for interaction at 100 m 0.05 for peanut and 0.06 for egg allergy.)ConclusionIncreased exposure to environmental greenness in the first year of life was associated with an increased risk of food allergy. Increased greenness may correlate with higher pollen levels which may trigger innate immune responses skewing the immune system to the Th2‐dependent allergic phenotype; additionally, some pollen and food allergens are cross‐reactive. Given the mixed data on greenness and other allergies, the relationship appears complex and may also be influenced by confounding variables outside those that were measured in this study.
Identification of a novel variant of FOXP3 resulting in severe immune dysregulation, polyendocrinopathy, enteropathy, X‐linked syndrome highlights potential pitfalls of molecular testing
Authors: Kirchner Allison , Sanchez Isabelle M. , Zalan Alice , Bhat Gifty , Bain Michelle 
Published: February 2022

AbstractImmune dysregulation, polyendocrinopathy, enteropathy, X‐linked (IPEX) syndrome is a rare genetic disorder that typically presents in the first year of life with severe diarrhea, autoimmune endocrine disorder, and inflammatory dermatitis, most commonly an eczematous dermatitis. IPEX syndrome is caused by variants in the FOXP3 gene leading to dysregulation of T‐regulatory (Treg) cells and an aberrant immune response. Here, we present a case of severe IPEX syndrome diagnosed following whole genome sequencing (WGS) in a 2‐week‐old boy with bloody mucoid diarrhea, failure to thrive, and a diffuse eczematous dermatitis. As multiple variants of interest were identified with WGS, this case highlights the importance of relating the clinical symptoms to the genetic results.
Enhanced Accumulation of Biologically Active Coumarin and Furanocoumarins in Callus Culture and Field‐grown Plants of Ruta chalepensis Through LED Light‐treatment
Authors: Juneja Kriti , Beuerle Till , Sircar Debabrata 
Published: February 2022

AbstractRuta chalepensis, a medicinal plant, produces biologically active coumarins (CRs) and furanocoumarins (FCRs). However, their yield is quite low in cultivated plants. In this work, the influence of light‐emitting diodes (LEDs) was investigated on the accumulation of CRs and FCRs in the callus cultures and field‐grown plants of R. chalepensis. Among the various tested wavelengths of LED lights, maximum accumulation of CR and FCRs was recorded under blue LED treatment in both the callus cultures as well as field‐grown plants when compared with respective controls treated with white LED. Metabolite analyses of LED‐treated field‐grown plants showed that highest concentrations of CR (umbelliferone, 2.8‐fold), and FCRs (psoralen, 2.3‐fold; xanthotoxin, 3.8‐fold and bergapten, 1.16‐fold) were accumulated upon blue LED‐treatment for 6 days. CR and FCRs contents were also analyzed in the blue LED‐ and red LED‐treated in vitro callus tissue. Upon blue LED‐treatment, callus accumulated significantly high levels of umbelliferone (48.6 ± 1.2 µg g−1 DW), psoralen (370.12 ± 10.6 µg g−1 DW) and xanthotoxin (10.16 ± 0.48 µg g−1 DW). These findings imply that blue LED‐treatment is a viable option as a noninvasive and low‐cost elicitation technology for the enhanced production of biologically active CR and FCRs in field‐grown plants and callus cultures of R. chalepensis.
Differential equations for the recurrence coefficients of semiclassical orthogonal polynomials and their relation to the Painlevé equations via the geometric approach
Authors: Dzhamay Anton , Filipuk Galina , Stokes Alexander 
Published: February 2022

AbstractIn this paper, we present a general scheme for how to relate differential equations for the recurrence coefficients of semiclassical orthogonal polynomials to the Painlevé equations using the geometric framework of the Okamoto Space of Initial Conditions. We demonstrate this procedure in two examples. For semiclassical Laguerre polynomials appearing in Ref. 1, we show how the recurrence coefficients are connected to the fourth Painlevé equation. For discrete orthogonal polynomials associated with the hypergeometric weight appearing in Ref. 2, we discuss the relation of the recurrence coefficients to the sixth Painlevé equation, extending the results of Ref. 3, where a similar approach was used for a discrete system for the same recurrence coefficients. Though the discrete and differential systems here share the same geometry, the construction of the space of initial conditions from the differential system is different and reveals extra considerations that must be made. We also discuss a number of related topics in the context of the geometric approach, such as Hamiltonian forms of the differential equations for the recurrence coefficients, Riccati solutions for special parameter values, and associated discrete Painlevé equations.
Parental affective personality and children's self‐reported internalising and externalising behaviour
Authors: Thaw Amanda , Herba Catherine M. , Orri Massimiliano , Paquin Stéphane , Séguin Jean R. , Berthoz Sylvie , Kim‐Cohen Julia , Tremblay Richard E. , Côté Sylvana 
Published: February 2022

AbstractPast research has demonstrated associations between parental personality traits and children's behaviour. However, fathers have been largely excluded from this research, and mothers often rate both their own personality and their child's behaviour, contributing to shared method variance. This study contributes to the literature by examining associations between parental biologically based affective personality traits, analysed separately for mothers and fathers, and seven‐ and eight‐year‐old children's self‐reported internalising and externalising behaviours. Data were analysed for 272 mother‐child dyads and 208 father‐child dyads. A series of multiple linear regressions was utilised to test associations between mothers’ and fathers’ traits of ANGER, SADNESS, FEAR, PLAY, SEEKING and CARE, assessed using the Affective Neuroscience Personality Scales (ANPS), and children's self‐reported internalising and externalising behaviours, assessed using the Berkeley Puppet Interview. Results revealed that higher ANPS ANGER scores amongst mothers were associated with more externalising behaviours in boys. Mothers with higher scores on SEEKING had sons with lower scores on externalising behaviours, while mothers with higher PLAY scores had sons with lower scores on internalising behaviours. Fathers with higher ANPS SADNESS scores had children with greater internalising behaviours, while fathers with greater FEAR traits had children with lower internalising behaviours. Indirect associations through harsh or positive parenting were not significant. Findings demonstrated that ANPS traits of ANGER, PLAY and SEEKING for mothers and FEAR and SADNESS for fathers are associated with children's self‐reported externalising and internalising behaviours. This study adds to the literature on biologically based parental affective personality and children's internalising and externalising behaviours.
Gibberellins regulate ovule number through a DELLA–CUC2 complex in Arabidopsis
Authors: Barro‐Trastoy Daniela , Gomez Maria D. , Blanco‐Touriñán Noel , Tornero Pablo , Perez‐Amador Miguel A. 
Published: February 2022

SUMMARYOvule development is a key process for plant reproduction, helping to ensure correct seed production. Several molecular factors and plant hormones such as gibberellins are involved in ovule initiation and development. Gibberellins control ovule development by the destabilization of DELLA proteins, whereas DELLA activity has been shown to act as a positive factor for ovule primordia emergence. But the molecular mechanism by which DELLA acts in ovule primordia initiation remained unknown. In this study we report that DELLA proteins participate in ovule initiation by the formation of a protein complex with the CUC2 transcription factor. The DELLA protein GAI requires CUC2 to promote ovule primordia formation, through the direct GAI–CUC2 interaction in placental cells that would determine the boundary regions between ovules during pistil development. Analysis of GAI–CUC2 interaction and co‐localization in the placenta supports this hypothesis. Moreover, molecular analysis identified a subset of the loci for which the GAI protein may act as a transcriptional co‐regulator in a CUC2‐dependent manner. The DELLA–CUC2 complex is a component of the gene regulatory network controlling ovule primordia initiation in Arabidopsis.
Comparative antimicrobial efficacy of 4 surgical hand‐preparation procedures prior to application of an alcohol‐based hand rub in veterinary students
Authors: Viljoen Hermien , Schoeman Johan P. , Fosgate Geoffrey T. , Boucher Charles 
Published: February 2022

AbstractObjectiveTo determine the influence of skin preparations before application of an alcohol‐based hand rub (ABHR) on bacterial counts before and after elective surgery.Study designClinical prospective study.Sample populationVeterinary students (n = 103) performing ovariohysterectomies on 140 dogs.MethodsParticipants were randomly assigned to 1 initial surgical preparation on the day of surgery: A – hand preparation with medicated solution (4% w/v chlorhexidine bigluconate followed by an ABHR; B – application of a medication solution (benzalkonium chloride 0.1%‐1% and polymeric biguanide hydrochloride 0.01%‐0.1%) followed by an ABHR; C – nonmedicated pH‐neutral soap hand wash followed by ABHR, and D – direct application of an ABHR. Samples were taken by pressing the distal finger tips to an agar plate before the hand preparation, after the hand preparation (n = 3), after ABHR application, and 120 minutes later. Colony‐forming units (CFUs) for samples were determined. Total log CFU and CFU log10 reduction were calculated and used for comparison with P < .05.ResultsTwo hours after surgery commenced, the participants of groups that performed a hand preparation had lower total CFUs than those that did not perform a hand preparation (P = .001). In particular, the number of CFUs was lower when ABHR was performed after application of pHN compared to direct ABHR (P = .001).ConclusionIn this population, performing a hand preparation with pHN prior to applying an ABHR had better antimicrobial effect for the duration of surgery than not performing a hand preparation.Clinical significanceSurgeons should wash their hands prior to ABHR before starting their first surgery of the day, even when hands appear clean.
Injuries in Masters Field Hockey Players Competing in the 2018 World Cup: Comparisons of Prevalence, Location, and Type by Age, Sex, and Field Hockey Experience
Authors: Croteau Karen , Eduljee Nina B. , Murphy Laurie , Rosene John 
Published: February 2022

This study examined prevalence, body location, and type of injuries reported by Masters Field Hockey players competing in the 2018 Masters and Grand Masters Field Hockey World Cups. There were 465 participants (284 females and 181 males) from 21 countries, ranging in age from 35 to 76 years. Participants completed The Health and Well-being of Masters Field Hockey Athletes Survey, which included injury type and location. The lower leg was the most common injury location and muscle strain the most common type of injury. Significant differences were found between females and males in upper extremity injuries and contusions.