bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non‐canonical ORFs‐derived protein products in mitochondria: A multifaceted exploration of their functions in health and disease
Authors: Ajala Ikram , Vanderperre Benoît 
Published: February 2025

AbstractTraditionally, eukaryotic mRNAs were perceived as inherently monocistronic. However, recent insights from ribosome profiling (Ribo‐seq) and proteomics studies challenge this paradigm. These investigations reveal that, beyond the currently annotated reference proteins (RefProts), there exist additional proteins known as alternative proteins (AltProts) and small open reading frames derived microproteins encoded in regions of mRNAs previously considered untranslated or in non‐coding transcripts. This experimental evidence broadens the spectrum of functional proteins within cells, tissues, and organs, potentially offering crucial insights into biological processes. Notably, a significant proportion of these newly identified AltProts and microproteins demonstrates localization in mitochondria, contributing to the functions of mitochondrial complexes. This review delves into the overlooked realm of the alternative proteome within mitochondria, exploring the role of nuclear or mitochondrial‐genome‐encoded AltProts and microproteins in physiological and pathological cellular processes.
Insights into the low‐temperature adaptation of an enzyme as studied through ancestral sequence reconstruction
Authors: Cui Shuang , Dasgupta Subrata , Yagi Sota , Kimura Madoka , Furukawa Ryutaro , Tagami Shunsuke , Akanuma Satoshi 
Published: February 2025

AbstractFor billions of years, enzymes have evolved in response to the changing environments in which their host organisms lived. Various lines of evidence suggest the earliest primitive organisms inhabited high‐temperature environments and possessed enzymes adapted to such conditions. Consequently, extant mesophilic and psychrophilic enzymes are believed to have adapted to lower temperatures during the evolutionary process. Herein, we analyzed this low‐temperature adaptation using ancestral sequence reconstruction. Previously, we generated the phylogenetic tree of 3‐isopropylmalate dehydrogenases (IPMDHs) and reconstructed the sequence of the last bacterial common ancestor. The corresponding ancestral enzyme displayed high thermostability and catalytic activity at elevated temperatures but moderate activity at low temperatures (Furukawa et al., Sci. Rep., 2020;10:15493). Here, to identify amino acid residues that are responsible for the low‐temperature adaptation, we reconstructed and characterized all 11 evolutionary intermediates that sequentially connect the last bacterial common ancestor with extant mesophilic IPMDH from Escherichia coli. A remarkable change in catalytic properties, from those suited for high reaction temperatures to those adapted for low temperatures, occurred between two consecutive evolutionary intermediates. Using a combination of sequence comparisons between ancestral proteins and site‐directed mutagenesis analyses, three key amino acid substitutions were identified that enhance low‐temperature catalytic activity. Intriguingly, amino acid substitutions that had the most significant impact on activity at low temperatures displayed no discernable effect on thermostability. However, these substitutions markedly reduced the activation energy for catalysis, thereby improving low‐temperature activity. The results were further investigated by molecular dynamics simulations of the predicted structures of the ancestral enzymes. Our findings exemplify how ancestral sequence reconstruction can identify residues crucial for adaptation to low temperatures.
Different nontarget‐site mechanisms underlie resistance to dicamba and 2,4‐D in an Amaranthus tuberculatus population
Authors: Werle Isabel Schlegel , Bobadilla Lucas Kopecky , Raiyemo Damilola A , Lopez Alexander J , Mesquita Machado Filipi , Tranel Patrick J 
Published: February 2025

AbstractBACKGROUNDAmaranthus tuberculatus (Moq.) Sauer (waterhemp) has emerged as one of several weed species that is resistant to synthetic auxin herbicides (SAHs). Among the mechanisms of resistance to SAHs, nontarget‐site resistance (NTSR) has been of particular concern owing to its complexity. Here, we integrated linkage mapping with transcriptome analysis to explore NTSR mechanisms to two SAHs, dicamba and 2,4‐D, in a multiple‐herbicide‐resistant A. tuberculatus population (CHR).RESULTSPhenotypic evaluations of an F2 mapping population indicated a polygenic basis for both dicamba and 2,4‐D resistance in CHR. A weak correlation was observed between phenotypic responses to dicamba and 2,4‐D treatments. Linkage mapping analyses revealed eight quantitative trait loci (QTL) regions associated with dicamba and 2,4‐D resistance mapped to seven A. tuberculatus chromosomes. Together, these QTL regions explained 24.2 and 23.1% of the variation in dicamba‐ and 2,4‐D‐resistant phenotypes, respectively. Only one co‐localized QTL region was found between the two resistance traits.CONCLUSIONThe results of this study demonstrated that resistance to dicamba and 2,4‐D in the CHR population is under the control of genes at multiple loci. The weak phenotypic and genetic associations of resistance traits indicate that more than one NTSR mechanism confers resistance to dicamba and 2,4‐D in this A. tuberculatus population. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Intraseasonal synchronization of extreme rainfall between North India and the Sahel
Authors: Strnad Felix M. , Hunt Kieran M.R. , Boers Niklas , Goswami Bedartha 
Published: February 2025

AbstractThe Indian Summer Monsoon (ISM) and the West African Monsoon (WAM) are dominant drivers of boreal summer precipitation variability in tropical and subtropical regions. Although the regional precipitation dynamics in these two regions have been extensively studied, the intraseasonal interactions between the ISM and WAM remain poorly understood. Here, we employ a climate network approach based on extreme rainfall events to uncover synchronously occurring extreme rainfall patterns across the two monsoon systems. We reveal strong synchronization of extreme rainfall events during the peak monsoon period in July and August, linking heavy rainfall over North India to that over the Sahel with a lag of around 12 days. We find that La Niña like conditions in combination with the Boreal Summer Intraseasonal Oscillation (BSISO) and an enhanced Tropical Easterly Jet (TEJ) foster the synchronization between the ISM and the WAM. The northward‐propagating BSISO triggers African Easterly Wave activity, bounded between a reduced African Easterly Jet and the TEJ. These induce westward‐traveling anomalous deep convection over the Sahel, which causes extreme rainfall events to occur.
Endobronchial Leiomyoma Presenting as Post‐Obstructive Pneumonia
Authors: Ng Boon Hau , Low Hsueh Jing , Abeed Nik Nuratiqah Nik , Sharil Nor Safiqah , Osman Rose Azzlinda , Krishnasamy Maalini , Ban Andrea Yu‐Lin , Rahaman Jamalul Azizi Abdul 
Published: February 2025

ABSTRACTEndobronchial leiomyoma is a rare airway tumour. We report the case of a 30‐year‐old female non‐smoker who presented with persistent fever and cough, ultimately diagnosed with post‐obstructive pneumonia secondary to an endobronchial leiomyoma. The patient underwent a successful endobronchial cryo‐resection with the resolution of symptoms. This case highlights the importance of considering rare causes of non‐resolving pneumonia and the role of a minimally invasive approach in the management of endobronchial leiomyoma.
Lung Mass With Crazy‐Paving: An Unusual Initial Presentation of Acute Myeloid Leukaemia
Authors: Woo Fui Bee , Tan Han Loong , Maria Dass Raymund Dass , Sivandan Shobhana 
Published: February 2025

ABSTRACTAcute myeloid leukaemia is known to present non‐specifically and more commonly with B symptoms. We report an interesting presentation of acute myeloid leukaemia as a lung mass which initially raised clinical suspicion of primary lung cancer. A gentleman in his 50s who is an active smoker presented with chronic cough, lethargy, and giddiness for 2 months. Physical examination revealed pallor, cervical lymphadenopathy, and bronchial breathing over the right middle zone on auscultation. CT thorax showed a spiculated solid mass with a crazy‐paving pattern, suggestive of lung cancer. However, blood workup showed pancytopenia with a full blood picture showing 60% blast cells. Subsequent bone marrow examination confirmed the diagnosis of AML. The patient received 2‐weekly venetoclax‐based chemotherapy. Serial follow‐up CT showed resolution of lung changes and the patient remained in remission.
Engineering in NIR‐responsive photothermal materials and their application in wound healing administration
Authors: Bassam Alsharafi , Du Mengxuan , Li Yuan , He Xiaojun 
Published: February 2025

AbstractWound healing is a complex process that involves multiple stages and is susceptible to various challenges, such as infection, insufficient blood flow, and the body's inadequate response to the healing process, which can be life‐threatening for the patient. In the current medical landscape, traditional treatments often struggle to meet the high standards of modern medical care. Therefore, it is crucial to actively explore and develop new drugs or advanced technologies that can enhance the healing of bacterially infected wounds. In recent years, several physical methods that effectively accelerate wound healing have garnered widespread attention and interest. Among these, Photothermal therapy (PTT) has been extensively utilized in the treatment of bacterial infections and wound healing due to its non‐invasive, low toxicity, and ease of use. The photothermal agents (PTAs) serve as the core material in PTT, and their efficacy is significantly influenced by the specific PTAs employed. Selecting the appropriate PTAs is essential for achieving the desired therapeutic effect. This treatment relies on the PTAs's ability to efficiently convert specific wavelengths of light energy into heat upon absorption, thereby generating a thermal effect at the wound site. Consequently, the properties of the PTAs, including photothermal conversion efficiency, biocompatibility, and stability within the organism, are critical factors that determine the therapeutic outcome. This review introduces organic, inorganic, and organic‐inorganic hybrid PTAs for PTT in wound healing, highlighting their main properties and applications in wound management in recent years. Finally, we briefly discuss the limitations and prospects of this field.
Efficient and Stable Synthesis of Butyrolactone from Biomass Derived 1,4‐ButaneDiol Dehydrogenation over Cu Supported on MgAl2O4 with Textural Properties
Authors: Ramudu Manne Parusha , Jagadish T. , Eshwari K. , Swapna S. , Ambedkar G. , Sunithamanjari Padma , Nagaiah Peddinti 
Published: February 2025

AbstractCopper based catalysts have gained significant attention due to the exceptional reactivity of Cu species in breaking OH and C─H bonds through oxidation, as well as their cost‐effectiveness. Herein, a series of Cu‐based MgAl2O4 catalysts with high dispersed Cu‐based spinel oxide particles were synthesized by the impregnation method and tested for catalytic dehydrogenation of 1,4‐Butanediol to gamma butyrolactone. The 15 MgAl2O4 catalysts confirmed the highest activity and the maximum stability among all catalysts. Under moderate reaction conditions, a 1,4‐Butanediol conversion of 98% with a gamma butyrolactone selectivity of 99% were achieved on the 15 MgAl2O4 catalysts. XRD, H2‐TPR, TPD, BET, FT‐IR, and N2O pulse characterization exposed that the catalyst prepared by the impregnation method could be ascribed to the smallest Cu particle size, high dispersion, proper surface acid‐base sites and the weak metal‐support interaction, leading to the highest performance.
β‐ and γ‐Amino Acids‐Containing Cu(II) Coordination Complexes: Synthesis, Structure, and Antibacterial Activity
Authors: Gumtya Milan , Konar Aditi , Haldar Debasish 
Published: February 2025

AbstractTwo coordination complexes of Cu(II) with N‐phthaloyl‐β‐alanine and N‐phthaloyl‐γ‐aminobutyric acid have been achieved in this work. The Cu(II) complexes have been characterized and studied by FT‐IR, absorption and emission spectroscopy, mass spectrometry, EPR, TGA, and FE‐SEM. The crystallographic structural descriptions along with the antibacterial activity of these two coordination complexes have been explored. From X‐ray crystallography, in complex 1, the Cu(II)‐center shows a penta‐coordinated geometry with oxygen donor sites from two N‐phthaloyl‐β‐alanine, two pyridines, along with one coordinated water molecule. In complex 2, the Cu(II)‐center shows a hexa‐coordinated geometry with oxygen donor sites from two N‐phthaloyl‐γ‐aminobutyric acid and two pyridines. The complexes adopt a paddle‐wheel conformation that eventually forms a porous structure in higher‐order packing through multiple π‐π stacking interactions. We have also investigated their inhibitory effects against both gram‐negative (Escherichia coli) and gram‐positive (Bacillus subtilis) bacteria. The Cu(II) complex with N‐phthaloyl‐β‐alanine showed the highest inhibitory effects. Gaining insight into the atomic level details can help design tailor‐made coordination complexes with enhanced antibacterial performance.
Applications of Magnetic Nanocomposites and Their Role in Advancing Lateral Flow Assays
Authors: Sanattalab Ehsan , Ayni Ezgi , Kaya Kubra , Yildirim‐Tirgil Nimet 
Published: February 2025

AbstractThis article explores the wide‐ranging applications of magnetic nanocomposites and their critical role in advancing lateral flow assays (LFAs) as diagnostic tools. We begin by reviewing a diverse array of magnetic nanomaterials, including magnetic plasmonic, polymer, carbon, and silica‐based nanocomposites, as well as notable examples such as CoFe₂O₄, magnetic quantum dots, lipid‐based nanoparticles, hydrogels, metal–organic frameworks (MOFs), superparamagnetic iron oxide nanoparticles (SPIONs), and gold nanorods. Each type of nanocomposite is examined for its unique properties and significant potential in enhancing sensitivity, stability, and multifunctionality. These nanocomposites are used across various sectors, such as medical diagnostics, environmental monitoring, and imaging technology, where they contribute to improved signal strength, binding affinity, and biocompatibility. The article focuses specifically on the application of magnetic nanocomposites in LFAs. These assays rely on magnetic nanoparticles’ interactions with target molecules to achieve detection, providing a promising platform for point‐of‐care diagnostics. The integration of magnetic nanoparticles enhances detection limits, improves precision, and reduces response times, making them an invaluable component in rapid diagnostic tests. By embedding magnetic nanocomposites into LFAs, researchers and clinicians can achieve more reliable and accurate results, facilitating early diagnosis and ultimately improving patient outcomes. Their integration into LFAs demonstrates significant potential for various diagnostic applications, from early disease detection to real‐time environmental monitoring, suggesting a transformative impact on point‐of‐care diagnostics and beyond.
Electrochemical Investigation of DNA Interactions with Cyanobacterial Toxins: Cylindrospermopsin and Saxitoxin
Authors: Kesici‐Meco Ece , Yilmaz Mete , Unur‐Yilmaz Ece 
Published: February 2025

AbstractAnthropogenic eutrophication threatens freshwater sources used for agricultural irrigation and increases the occurrence risk of cyanotoxins, such as cylindrospermopsin (CYN) and saxitoxin (STX). These toxins accumulate in surface waters and pose significant health risks, with CYN primarily affecting the liver and STX targeting the nervous system. In this study, dsDNA was immobilized on a pencil graphite electrode (PGE), and its interactions with CYN and STX were investigated by means of electrochemical impedance spectroscopy (EIS). Experimental conditions, including DNA concentration, cyanotoxin concentration, and DNA‐cyanotoxin interaction time, were optimized to generate the most significant electrochemical signal (i.e., charge transfer resistance). The optimized PGE/DNA system established the foundation for an electrochemical biosensor that can be utilized for detection of cyanotoxins and detection limits of 0.12 ng mL−1 and 0.043 ng mL−1 were achieved for CYN and STX, respectively. The system was further analyzed using FTIR, UV–vis, and X‐ray photoelectron spectroscopy (XPS), providing a comprehensive structural analysis of toxin‐induced DNA alterations. The electrochemical system developed in this study by immobilizing DNA onto PGE allowed thorough investigation of the genotoxic effects of CYN and STX, as well as their sensitive detection.
Spatial Confinement and Induced Deposition of ZnHCF in 3D Structure for Ultrahigh‐Rate and Dendrite‐Free Zn Anodes
Authors: Ding Gang , Yang Qiushao , Zhang Wenyuan , Fu Jinwen , Tong Renle , Feng Yongbao , Gong Wenbin , Xue Pan , Li Qiulong 
Published: February 2025

AbstractAqueous Zn‐metal batteries (AZBs) are thought as highly prospective candidates for large‐scale energy‐storage systems because of their abundant natural resources, low cost, high safety, and environmentally friendly. Nevertheless, the key problems of AZBs are the uncontrollable zinc dendrites growth and water‐induced erosion faced by zinc anodes. Therefore, reducing the hydrophilicity of zinc anode and introducing the zincophilic sites are the availably strategy. Herein, 3D highly‐conductive host is developed to inhibit Zn dendrites growth, which have a porous structure consisting of graphene and carbon nanotubes embedded with a zincophilic nucleation sites of Zn Prussian blue analogs (ZnHCF@3D‐GC). The inner ZnHCF possess minimized nucleation barriers, which can serve as favorable nucleation sites, and 3D host provide a buffer interspace to allow for even more high‐capacity Zn plating. Additionally, density functional theory results show that ZnHCF exhibits a strong Zn binding energy and high adsorption energy of Zn (002) plane, which can guide Zn horizontal deposition in the 3D host. As a result, the assembled symmetrical cell is able to stabilize 900 cycles at an ultrahigh current density of 100 mA cm−2. Zn‐ZnHCF@3D‐GC//MnO2 and Zn‐ZnHCF@3D‐GC//ZnHCF full cells can be stably cycled 1000 cycles at 2.0 A g−1.
Global Prevalence of Anaplasma phagocytophilum in Cattle: A One Health Perspective, Meta‐Analysis and Future Predictions (up to 2035)
Authors: Abdoli Amir , Olfatifar Meysam , Zaki Leila , Nikkhahi Farhad , Fardsanei Fatemeh , Sobhani Sona , Sadeghi Hamid , Eslahi Aida Vafae , Badri Milad 
Published: February 2025

ABSTRACTAnaplasma phagocytophilum is an emerging tick‐borne zoonotic bacterium, which is considered a significant risk to the health and industry of cattle in tropical and sub‐tropical regions worldwide. This research focuses on examining the worldwide occurrence of A. phagocytophilum in cattle. Several databases, including Scopus, PubMed, ProQuest, Web of Science, ScienceDirect, and Google Scholar, were searched for publications spanning October 2004 to November 2024. The pooled prevalence was calculated with a 95% confidence interval (CI) using a random‐effects model based on the Freeman‐Tukey double arcsine transformation. A total of 72 studies satisfied the inclusion criteria, revealing a global prevalence of A. phagocytophilum in cattle estimated at 8.5% (5.9%–11.5%). Mongolia (51.9%, 45.9%–56.2%) and Guatemala (51%, 41.2%–60.7%) were countries that accounted for the highest prevalence. Moreover, the infection was most prevalent in African region with prevalence of 11.3% (3.9%–21.5%). The highest prevalence rate was observed in hot‐summer Mediterranean climate (13.7%, 4.7%–26.2%). The analysis indicated that immunological techniques were associated with the highest prevalence rate (14.2%, 6.5%–24.3%). The findings of the present research highlighted important geographical and environmental factors that affect the prevalence of disease. In the fields of veterinary medicine and public health, these findings enhance disease management plans and preventative initiatives.
Ein Abszess auf Abwegen
Authors: Seidl Hatti , Thormählen Lena , Pollak Marie , Walter-Rittel Thula , Hättasch Robert , Hindricks Gerhard 
Published: February 2025

Zusammenfassung
Eine 69-jährige Patientin mit bekanntem Rezidiv eines Ösophaguskarzinoms wurde mit Fieber und erhöhten Entzündungswerten vorstellig. In der jüngeren Vergangenheit war ein suspekter mediastinaler Lymphknoten bronchoskopisch biopsiert worden. In Folge entwickelte sich ein Lymphknotenabszess, der über eine Fistel in die V. cava superior eine Blutstrominfektion (Erreger: Streptococcus anginosus) verursachte. Diese streute über einen vorbestehenden Atriumseptumdefekt bis nach zerebral, wo es zur Entwicklung multipler Hirnabszesse kam.
Plasma free hemoglobin is associated with LDH, AST, total bilirubin, reticulocyte count, and the hemolysis score in patients with sickle cell anemia
Authors: Liu Angela , Jacobs-McFarlane Charleen , Sebastiani Paola , Glassberg Jeffrey , McCuskee Sarah , Curtis Susanna 
Published: February 2025

Abstract
Plasma free hemoglobin (PFH) is a direct biomarker for hemolysis that has been associated with clinical complications such as pulmonary hypertension and death in patients with sickle cell disease (SCD). We sought to characterize the relationship between PFH and more clinically available hemolytic markers including lactate dehydrogenase (LDH), aspartate aminotransferase (AST), bilirubin, reticulocyte percentage and to derive a composite hemolysis score derived from principal component analysis (PCA) of these biomarkers. In 68 adult patients (median age 31 years old, IQR 25–39) with HbSS or HbSβ0-thalassemia enrolled in the IMPROVE II study, median PFH was elevated at 21.9 mg/dL (IQR 9.9–44.9 mg/dL) at steady state. Using Pearson correlation analysis, PFH had a stronger relationship to LDH (R = 0.699), AST (R = 0.587), and total bilirubin (R = 0.475), compared to reticulocyte count (R = 0.316). The hemolysis score was significantly associated with PFH (R = 0.677). When compared with other laboratory measures, PFH correlated with hemoglobin (R= -0.275) and HbS (R = 0.277), but did not correlate with white blood cell count (WBC) or HbF. The hemolysis score was significantly associated with WBC (R = 0.307), hemoglobin (R = -0.393), HbF (R=- 0.424), and HbS (R = 0.423). This study confirms that the conventional hemolytic biomarkers LDH, AST, bilirubin, and reticulocyte percentage correlate with PFH. Additionally, the hemolysis score is a valid tool to measure hemolysis and that it may be a marker of global hemolysis as opposed to PFH, which quantifies intravascular hemolysis. Further studies will be needed to elucidate the role of PFH and intravascular hemolysis in the development of clinical complications of sickle cell disease. Statements and Funding Declarations: The research leading to these results received funding from the National Heart, Lung, and Blood Institute (NHLBI) R01 HL142671 Grant under J.G. J.G. has also served as a consultant for CSL Behring, Novartis, and Novo Nordisk synteract DSMB and is supported by NHLBI RO1HL159116, R01 HL142671, R01 ES030717, UG1 HL138645, UH3 HL143192, U01HL167036, and the Doris Duke Charitable Foundation Advancing Cures grant. S.C. has served as a consultant for Pfizer and is supported by the NHLBI 5K23HL151884 grant. A.L. is supported by the NHLBI 5T32HL129974-05. C.J.M is supported by the NHLBI 5T32HL129974-05. P.S., and S.M. declare no conflicts and/or funding.