bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a skin- and neuro-attenuated live vaccine for varicella
Authors: Wang Wei , Pan Dequan , Fu Wenkun , Ye Xiangzhong , Han Jinle , Yang Lianwei , Jia Jizong , Liu Jian , Zhu Rui , Zhang Yali , Liu Che , Ye Jianghui , Selariu Anca , Que Yuqiong , Zhao Qinjian , Wu Ting , Li Yimin , Zhang Jun , Cheng Tong , Zhu Hua , Xia Ningshao 
Published: February 2022

AbstractVaricella caused by the primary infection of varicella-zoster virus (VZV) exerts a considerable disease burden globally. Current varicella vaccines consisting of the live-attenuated vOka strain of VZV are generally safe and effective. However, vOka retains full neurovirulence and can establish latency and reactivate to cause herpes zoster in vaccine recipients, raising safety concerns. Here, we rationally design a live-attenuated varicella vaccine candidate, v7D. This virus replicates like wild-type virus in MRC-5 fibroblasts and human PBMCs, the carrier for VZV dissemination, but is severely impaired for infection of human skin and neuronal cells. Meanwhile, v7D shows immunogenicity comparable to vOka both in vitro and in multiple small animal species. Finally, v7D is proven well-tolerated and immunogenic in nonhuman primates. Our preclinical data suggest that v7D is a promising candidate as a safer live varicella vaccine with reduced risk of vaccine-related complications, and could inform the design of other herpes virus vaccines.
Data-informed discovery of hydrolytic nanozymes
Authors: Li Sirong , Zhou Zijun , Tie Zuoxiu , Wang Bing , Ye Meng , Du Lei , Cui Ran , Liu Wei , Wan Cuihong , Liu Quanyi , Zhao Sheng , Wang Quan , Zhang Yihong , Zhang Shuo , Zhang Huigang , Du Yan , Wei Hui 
Published: February 2022

AbstractNanozyme is a collection of nanomaterials with enzyme-like activity but higher environmental tolerance and long-term stability than their natural counterparts. Improving the catalytic activity and expanding the category of nanozymes are prerequisites to complement or even supersede enzymes. However, the development of hydrolytic nanozymes is still challenged by diverse hydrolytic substrates and following complicated mechanisms. Here, two strategies are informed by data to screen and predict catalytic active sites of MOF (metal–organic framework) based hydrolytic nanozymes: (1) to increase the intrinsic activity by finely tuned Lewis acidity of the metal clusters; (2) to improve the density of active sites by shortening the length of ligands. Finally, as-obtained Ce-FMA-MOF-based hydrolytic nanozyme is capable of cleaving phosphate bonds, amide bonds, glycosidic bonds, and even their mixture, biofilms. This work provides a rational methodology to design hydrolytic nanozyme, enriches the diversity of nanozymes, and potentially sheds light on future evolution of enzyme engineering.
Deep learning-based high-accuracy quantitation for lumbar intervertebral disc degeneration from MRI
Authors: Zheng Hua-Dong , Sun Yue-Li , Kong De-Wei , Yin Meng-Chen , Chen Jiang , Lin Yong-Peng , Ma Xue-Feng , Wang Hong-Shen , Yuan Guang-Jie , Yao Min , Cui Xue-Jun , Tian Ying-Zhong , Wang Yong-Jun 
Published: February 2022

AbstractTo help doctors and patients evaluate lumbar intervertebral disc degeneration (IVDD) accurately and efficiently, we propose a segmentation network and a quantitation method for IVDD from T2MRI. A semantic segmentation network (BianqueNet) composed of three innovative modules achieves high-precision segmentation of IVDD-related regions. A quantitative method is used to calculate the signal intensity and geometric features of IVDD. Manual measurements have excellent agreement with automatic calculations, but the latter have better repeatability and efficiency. We investigate the relationship between IVDD parameters and demographic information (age, gender, position and IVDD grade) in a large population. Considering these parameters present strong correlation with IVDD grade, we establish a quantitative criterion for IVDD. This fully automated quantitation system for IVDD may provide more precise information for clinical practice, clinical trials, and mechanism investigation. It also would increase the number of patients that can be monitored.
Heterogeneous repolarization creates ventricular tachycardia circuits in healed myocardial infarction scar
Authors: Kelemen Kamilla , Greener Ian D. , Wan Xiaoping , Parajuli Shankar , Donahue J. Kevin 
Published: February 2022

AbstractArrhythmias originating in scarred ventricular myocardium are a major cause of death, but the underlying mechanism allowing these rhythms to exist remains unknown. This gap in knowledge critically limits identification of at-risk patients and treatment once arrhythmias become manifest. Here we show that potassium voltage-gated channel subfamily E regulatory subunits 3 and 4 (KCNE3, KCNE4) are uniquely upregulated at arrhythmia sites within scarred myocardium. Ventricular arrhythmias occur in areas with a distinctive cardiomyocyte repolarization pattern, where myocyte tracts with short repolarization times connect to myocytes tracts with long repolarization times. We found this unique pattern of repolarization heterogeneity only in ventricular arrhythmia circuits. In contrast, conduction abnormalities were ubiquitous within scar. These repolarization heterogeneities are consistent with known functional effects of KCNE3 and KCNE4 on the slow delayed-rectifier potassium current. We observed repolarization heterogeneity using conventional cardiac electrophysiologic techniques that could potentially translate to identification of at-risk patients. The neutralization of the repolarization heterogeneities could represent a potential strategy for the elimination of ventricular arrhythmia circuits.
The spatial transcriptomic landscape of the healing mouse intestine following damage
Authors: Parigi Sara M. , Larsson Ludvig , Das Srustidhar , Ramirez Flores Ricardo O. , Frede Annika , Tripathi Kumar P. , Diaz Oscar E. , Selin Katja , Morales Rodrigo A. , Luo Xinxin , Monasterio Gustavo , Engblom Camilla , Gagliani Nicola , Saez-Rodriguez Julio , Lundeberg Joakim , Villablanca Eduardo J. 
Published: February 2022

AbstractThe intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions, we demonstrate a previously unappreciated molecular regionalization of the colon, which dramatically changes during mucosal healing. Here, we identified spatially-organized transcriptional programs defining compartmentalized mucosal healing, and regions with dominant wired pathways. Furthermore, we showed that decreased p53 activation defined areas with increased presence of proliferating epithelial stem cells. Finally, we mapped transcriptomics modules associated with human diseases demonstrating the translational potential of our dataset. Overall, we provide a publicly available resource defining principles of transcriptomic regionalization of the colon during mucosal healing and a framework to develop and progress further hypotheses.
Micro-mechanical blood clot testing using smartphones
Authors: Chan Justin , Michaelsen Kelly , Estergreen Joanne K. , Sabath Daniel E. , Gollakota Shyamnath 
Published: February 2022

AbstractFrequent prothrombin time (PT) and international normalized ratio (INR) testing is critical for millions of people on lifelong anticoagulation with warfarin. Currently, testing is performed in hospital laboratories or with expensive point-of-care devices limiting the ability to test frequently and affordably. We report a proof-of-concept PT/INR testing system that uses the vibration motor and camera on smartphones to track micro-mechanical movements of a copper particle. The smartphone system computed the PT/INR with inter-class correlation coefficients of 0.963 and 0.966, compared to a clinical-grade coagulation analyzer for 140 plasma samples and demonstrated similar results for 80 whole blood samples using a single drop of blood (10 μl). When tested with 79 blood samples with coagulopathic conditions, the smartphone system demonstrated a correlation of 0.974 for both PT/INR. Given the ubiquity of smartphones in the global setting, this proof-of-concept technology may provide affordable and effective PT and INR testing in low-resource environments.
PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection
Authors: Wang Shifan , Guo Huijuan , Zhu-Salzman Keyan , Ge Feng , Sun Yucheng 
Published: February 2022

AbstractApoptosis and autophagy are two common forms of programmed cell death (PCD) used by host organisms to fight against virus infection. PCD in arthropod vectors can be manipulated by arboviruses, leading to arbovirus-vector coexistence, although the underlying mechanism is largely unknown. In this study, we find that coat protein (CP) of an insect-borne plant virus TYLCV directly interacts with a phosphatidylethanolamine-binding protein (PEBP) in its vector whitefly to downregulate MAPK signaling cascade. As a result, apoptosis is activated in the whitefly increasing viral load. Simultaneously, the PEBP4-CP interaction releases ATG8, a hallmark of autophagy initiation, which reduces arbovirus levels. Furthermore, apoptosis-promoted virus amplification is prevented by agonist-induced autophagy, whereas the autophagy-suppressed virus load is unaffected by manipulating apoptosis, suggesting that the viral load is predominantly determined by autophagy rather than by apoptosis. Our results demonstrate that a mild intracellular immune response including balanced apoptosis and autophagy might facilitate arbovirus preservation within its whitefly insect vector.
Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe
Authors: Li Lizhu , Lu Lihui , Ren Yuqi , Tang Guo , Zhao Yu , Cai Xue , Shi Zhao , Ding He , Liu Changbo , Cheng Dali , Xie Yang , Wang Huachun , Fu Xin , Yin Lan , Luo Minmin , Sheng Xing 
Published: February 2022

AbstractOptogenetic methods provide efficient cell-specific modulations, and the ability of simultaneous neural activation and inhibition in the same brain region of freely moving animals is highly desirable. Here we report bidirectional neuronal activity manipulation accomplished by a wireless, dual-color optogenetic probe in synergy with the co-expression of two spectrally distinct opsins (ChrimsonR and stGtACR2) in a rodent model. The flexible probe comprises vertically assembled, thin-film microscale light-emitting diodes with a lateral dimension of 125 × 180 µm2, showing colocalized red and blue emissions and enabling chronic in vivo operations with desirable biocompatibilities. Red or blue irradiations deterministically evoke or silence neurons co-expressing the two opsins. The probe interferes with dopaminergic neurons in the ventral tegmental area of mice, increasing or decreasing dopamine levels. Such bidirectional regulations further generate rewarding and aversive behaviors and interrogate social interactions among multiple mice. These technologies create numerous opportunities and implications for brain research.
Sensitivity of the electronic and magnetic structures of cuprate superconductors to density functional approximations
Authors: Pokharel Kanun , Lane Christopher , Furness James W. , Zhang Ruiqi , Ning Jinliang , Barbiellini Bernardo , Markiewicz Robert S. , Zhang Yubo , Bansil Arun , Sun Jianwei 
Published: February 2022

AbstractWe discuss the crystal, electronic, and magnetic structures of La2−xSrxCuO4 (LSCO) for x = 0.0 and x = 0.25 employing 13 density functional approximations, representing the local, semi-local, and hybrid exchange-correlation approximations within the Perdew–Schmidt hierarchy. The meta-generalized gradient approximation (meta-GGA) class of functionals is found to perform well in capturing the key properties of LSCO, a prototypical high-temperature cuprate superconductor. In contrast, the localspin-density approximation, GGA, and the hybrid density functional fail to capture the metal-insulator transition under doping.
Identification of milk from different animal and plant sources by desorption electrospray ionisation high-resolution mass spectrometry (DESI-MS)
Authors: Hong Yunhe , Birse Nicholas , Quinn Brian , Montgomery Holly , Wu Di , Rosas da Silva Gonçalo , van Ruth Saskia M. , Elliott Christopher T. 
Published: February 2022

AbstractThis study used desorption electrospray ionisation mass spectrometry (DESI-MS) to analyse and detect and classify biomarkers in five different animal and plant sources of milk for the first time. A range of differences in terms of features was observed in the spectra of cow milk, goat milk, camel milk, soya milk, and oat milk. Chemometric modelling was then used to classify the mass spectra data, enabling unique or significant markers for each milk source to be identified. The classification of different milk sources was achieved with a cross-validation percentage rate of 100% through linear discriminate analysis (LDA) with high sensitivity to adulteration (0.1–5% v/v). The DESI-MS results from the milk samples analysed show the methodology to have high classification accuracy, and in the absence of complex sample clean-up which is often associated with authenticity testing, to be a rapid and efficient approach for milk fraud control.
Copper(II)-β-cyclodextrin immobilized on graphitic carbon nitride nanosheets as a highly effective catalyst for tandem oxidative amidation of benzylic alcohols
Authors: Ghafuri Hossein , Rashidizadeh Afsaneh , Gorab Mostafa Ghafori , Jafari Ghazaleh 
Published: February 2022

AbstractIn this study, an efficient catalyst based on graphitic carbon nitride nanosheets (CN) and copper(II) supported β-cyclodextrin (β-CD/Cu(II)) was synthesized and used for tandem oxidative amidation of benzylic alcohols. In this regard, CN was functionalized by β-CD/Cu(II) via 1,3-dibromopropane linker (CN-Pr-β-CD/Cu(II)). The prepared catalyst was characterized using FT-IR, XRD, FE-SEM, EDS, TGA, ICP-OES, BET, and TEM analyses. CN-Pr-β-CD/Cu(II) was subsequently applied in a direct oxidative amidation reaction and it was observed that different benzyl alcohols were converted to desire amides with good to excellent efficiency. This reaction was performed in the presence of amine hydrochloride salts, tert-butyl hydroperoxide (TBHP), and Ca2CO3 in acetonitrile (CH3CN) under nitrogen atmosphere. CN-Pr-β-CD/Cu(II) can be recycled and reused five times without significant reduction in reaction efficiency.
The role of temporal distance of the events on the spatiotemporal dynamics of mental time travel to one’s personal past and future
Authors: Colás-Blanco I. , Mioche J. , La Corte V. , Piolino P. 
Published: February 2022

AbstractMental time travel to personal past and future events shows remarkable cognitive and neural similarities. Both temporalities seem to rely on the same core network involving episodic binding and monitoring processes. However, it is still unclear in what way the temporal distance of the simulated events modulates the recruitment of this network when mental time-travelling to the past and the future. The present study explored the electrophysiological correlates of remembering and imagining personal events at two temporal distances from the present moment (near and far). Temporal distance modulated the late parietal component (LPC) and the late frontal effect (LFE), respectively involved in episodic and monitoring processes. Interestingly, temporal distance modulations differed in the past and future event simulation, suggesting greater episodic processing for near as opposed to far future situations (with no differences on near and far past), and the implementation of greater post-simulation monitoring processes for near past as compared to far past events (with high demands on both near and far future). These findings show that both past and future event simulations are affected by the temporal distance of the events, although not exactly in a mirrored way. They are discussed according to the increasing role of semantic memory in episodic mental time travel to farther temporal distances from the present.
Aged related human skin microbiome and mycobiome in Korean women
Authors: Kim Hye-Jin , Oh Han Na , Park Taehun , Kim Hanbyul , Lee Hyun Gee , An Susun , Sul Woo Jun 
Published: February 2022

AbstractWe examined differences in the skin microbiome of two separate age groups to find key microbial and skin physiological indicators associated with aging. We recruited healthy Korean women 19–28 years old (Y-group) and 60–63 years old (O-group) and evaluated their cheek and forehead skin microbiome, including bacteria and fungi. The microbiome was significantly different by age group, with bacterial and fungal communities displaying higher alpha-diversity in the O-group than in the Y-group. We identified amplicon sequence variants affiliated with Cutibacterium and Lactobacillus and fungi Malassezia restricta as microbial biomarkers showing significant differences between the Y and O-group. There are more microbial communities and metabolic processes related to skin health in the Y-group than in the O-group, and there are more microbial interactions to increase the stability of the network structure of the skin. Skin physical metadata, including transepidermal water loss and sebum content, differed by two age groups. The crucial skin microbes, skin physical parameters, and microbial network found through this research will be useful key indicators in associating skin aging and skin microbiome research.
Investigation of thermal performance of Maxwell hybrid nanofluid boundary value problem in vertical porous surface via finite element approach
Authors: Algehyne Ebrahem A. , El-Zahar Essam R. , Elhag S. H. , Bayones Fatimah S. , Nazir Umar , Sohail Muhammad , Kumam Poom 
Published: February 2022

AbstractThe study of thermo-physical characteristics is essential to observe the impact of several influential parameters on temperature and velocity fields. The transportation of heat in fluid flows and thermal instability/stability is a charming area of research due to their wider applications and physical significance because of their utilization in different engineering systems. This report is prepared to study thermal transportation in Maxwell hybrid nanofluid past over an infinite stretchable vertical porous sheet. An inclusion of hybrid nanofluid is performed to monitor the aspects of thermal transportation. Keeping in mind the advantages of thermal failure, non-Fourier theory for heat flux model is utilized. Aspects of external heat source are also considered. The mathematical formulation for the considered model with certain important physical aspects results in the form of coupled nonlinear PDEs system. The obtained system is reduced by engaging boundary layer approximation. Afterwards, transformations have been utilized to convert the modeled PDEs system into ODEs system. The converted nonlinear ODEs system is then handled via finite element method coded in symbolic computational package MAPLE 18.0. Grid independent survey is presented for the validation of used approach and the comparative analysis has been done to confirm the reliability of obtained solution. The obtained solution is discussed and physical aspects have been explored and recorded against numerous involved influential variables. Motion into hybrid nanoparticles and nanoparticles becomes slow down versus higher values of Forchheimer and Darcy’s porous numbers. Thermal growth is enhanced for the case of hybrid nano-structures rather than for case of nanofluid. Thickness regarding momentum layer is dominated for hybrid nanoparticles rather than case of nanoparticles.
Simultaneous realization of polarization conversion for reflected and transmitted waves with bi-functional metasurface
Authors: Huang Xiaojun , Ma Xia , Li Xuewen , Fan Jingdao , Guo Liang , Yang Helin 
Published: February 2022

AbstractManipulating the polarizations of electroagnetic waves by flexible and diverse means is desirable for myriad microwave systems. More recently, metasurfaces have emerged as promising alternatives to conventional polarization manipulation components because the flexibility of their geometry means that they can be arbitrarily customized. In this context, a bilayered metasurface is presented to simultaneously manipulate the polarized states of reflected and transmitted microwaves. Regardless of whether an incident electromagnetic wave is x-polarized or y-polarized, the reflected and transmitted waves are converted into their orthogonal waves at the operating frequency. The designed metasurface has a high polarization conversion rate, above 90%, for both normal and oblique incidences. Experimental results verify the correctness of the simulated results. Finally, the axial ratio and surface current distributions are employed to reveal the physics of the polarization manipulation. The proposed metasurface will be beneficial in the design of flexible and versatile polarization converters, has great potential for applications in polarization-controlled devices and is believed to be extendable to higher frequency regimes.
Congenital collagenopathies increased the risk of inguinal hernia developing and repair: analysis from a nationwide population-based cohort study
Authors: Chang Hao-Han , Juan Yung-Shun , Li Ching-Chia , Lee Hsiang-Ying , Chen Jian-Han 
Published: February 2022

AbstractHerein, we aimed to explore whether male patients with congenital collagen diseases had a higher risk of inguinal herniation than patients without these diseases. Data were retrospectively collected from the National Health Insurance Research Database of Taiwan. The study cohort included 1,801 male patients diagnosed with congenital collagen diseases based on the ICD-9 CM diagnostic codes; after propensity score matching, the control group comprised 6,493 men without congenital collagen diseases. The primary endpoint was inguinal hernia repair during the observation period. During a median follow-up period of 133.9 months, the risk of inguinal herniation in the collagen group was significantly higher than that in the control group (HR = 2.237, 95% CI 1.646–3.291, p < 0.001). This phenomenon was observed in patients younger than 18 years (HR: 3.040, 95% CI 1.819–5.083, p < 0.001) and in those aged 18–80 years (HR: 1.909, 95% CI 1.186–3.073, p < 0.001). Asian men with congenital collagen diseases are at a high risk of developing inguinal hernias, regardless of age. Detailed physical examination and patient education should be performed for these patients to prevent inguinal herniation.
Slow and soft passage through tipping point of the Atlantic Meridional Overturning Circulation in a changing climate
Authors: Kim Soong-Ki , Kim Hyo-Jeong , Dijkstra Henk A. , An Soon-Il 
Published: February 2022

AbstractPaleo-proxy records suggest that the Atlantic Meridional Overturning Circulation (AMOC) exhibits a threshold for an abrupt change, a so-called tipping point. A classical bifurcation theory, a basis of the tipping dynamics of AMOC implicitly assumes that the tipping point is fixed. However, when a system is subjected to time-varying forcing (e.g., AMOC exposed to ice meltwater) an actual tipping point can be overshot due to delayed tipping, referred to as the slow passage effect. Here, using an Earth system model of intermediate complexity and a low-order model with freshwater forcing, we show that the tipping point of AMOC is largely delayed by the slow passage effect. It causes a large tipping lag of up to 1300 years, and strongly relaxes the abruptness of tipping as well. We further demonstrate that the tipping modulation can actively occur in past, present, and future climates by quantifying the effect during Dansgaard-Oeschger events, meltwater pulse 1A (MWP-1A), and current Greenland ice sheet melting. The suggested slow passage effect may explain the observed lagged AMOC collapse to MWP-1A of about 1000 years and provides implications tipping risk in the future.