bluuebunny/crossref_metadata_2025_split huggingface dataset:
Broad-spectrum in vitro activity of macrophage infectivity potentiator inhibitors against Gram-negative bacteria and Leishmania major
Authors: Iwasaki Jua , Lorimer Donald D. , Vivoli-Vega Mirella , Kibble Emily A. , Peacock Christopher S. , Abendroth Jan , Mayclin Stephen J. , Dranow David M. , Pierce Phillip G. , Fox David , Lewis Maria , Bzdyl Nicole M. , Kristensen Sofie S. , Inglis Timothy J. J. , Kahler Charlene M. , Bond Charles S. , Hasenkopf Anja , Seufert Florian , Schmitz Jens , Marshall Laura E. , Scott Andrew E. , Norville Isobel H. , Myler Peter J. , Holzgrabe Ulrike , Harmer Nicholas J. , Sarkar-Tyson Mitali 
Published: February 2022

Abstract

Background
The macrophage infectivity potentiator (Mip) protein, which belongs to the immunophilin superfamily, is a peptidyl-prolyl cis/trans isomerase (PPIase) enzyme. Mip has been shown to be important for virulence in a wide range of pathogenic microorganisms. It has previously been demonstrated that small-molecule compounds designed to target Mip from the Gram-negative bacterium Burkholderia pseudomallei bind at the site of enzymatic activity of the protein, inhibiting the in vitro activity of Mip.

Objectives
In this study, co-crystallography experiments with recombinant B. pseudomallei Mip (BpMip) protein and Mip inhibitors, biochemical analysis and computational modelling were used to predict the efficacy of lead compounds for broad-spectrum activity against other pathogens.

Methods
Binding activity of three lead compounds targeting BpMip was verified using surface plasmon resonance spectroscopy. The determination of crystal structures of BpMip in complex with these compounds, together with molecular modelling and in vitro assays, was used to determine whether the compounds have broad-spectrum antimicrobial activity against pathogens.

Results
Of the three lead small-molecule compounds, two were effective in inhibiting the PPIase activity of Mip proteins from Neisseria meningitidis, Klebsiella pneumoniae and Leishmania major. The compounds also reduced the intracellular burden of these pathogens using in vitro cell infection assays.

Conclusions
These results indicate that Mip is a novel antivirulence target that can be inhibited using small-molecule compounds that prove to be promising broad-spectrum drug candidates in vitro. Further optimization of compounds is required for in vivo evaluation and future clinical applications.
Pharmacokinetics of bedaquiline in cerebrospinal fluid (CSF) in patients with pulmonary tuberculosis (TB)
Authors: Upton Caryn M , Steele Chanel I , Maartens Gary , Diacon Andreas H , Wiesner Lubbe , Dooley Kelly E 
Published: February 2022

Abstract

Background
With current treatment options most patients with CNS TB develop severe disability or die. Drug-resistant tuberculous meningitis is nearly uniformly fatal. Novel treatment strategies are needed. Bedaquiline, a potent anti-TB drug, has been reported to be absent from CSF in a single report.

Objectives
To explore the pharmacokinetics of bedaquiline and its M2 metabolite in the CSF of patients with pulmonary TB.

Patients and methods
Individuals with rifampicin-resistant pulmonary TB established on a 24 week course of treatment with bedaquiline underwent a lumbar puncture along with multiple blood sample collections over 24 h for CSF and plasma pharmacokinetic assessment, respectively. To capture the expected low bedaquiline and M2 concentrations (due to high protein binding in plasma) we optimized CSF collection and storage methods in vitro before concentrations were quantified via liquid chromatography with tandem MS.

Results
Seven male participants were enrolled, two with HIV coinfection. Using LoBind® tubes lined with a 5% BSA solution, bedaquiline and M2 could be accurately measured in CSF. Bedaquiline and M2 were present in all patients at all timepoints at concentrations similar to the estimated unbound fractions in plasma.

Conclusions
Bedaquiline and M2 penetrate freely into the CSF of pulmonary TB patients with a presumably intact blood–brain barrier. Clinical studies are urgently needed to determine whether bedaquiline can contribute meaningfully to the treatment of CNS TB.
A nested algorithm of truss topology optimization for maximum plastic shakedown loading capacity
Authors: Li Kai , Cheng Gengdong 
Published: February 2022

Abstract
This paper proposes a nested algorithm for truss topology optimization under plastic shakedown theory, which aims at maximizing the shakedown loading capacity. The nested algorithm consists of shakedown analysis and sensitivity-based optimization. Analytical sensitivity of shakedown load with respect to the design variables is derived in a concise form for the first time in conjunction with the adjoint variable scheme. A primal-dual shakedown algorithm is implemented for the determination of the shakedown load limit of structures and adjoint variables needed in the sensitivity analysis. The ground structure approach is applied to formulate the optimization problem for determining the optimized topology and cross-sections of the truss structure. Based on the analytical sensitivity above, the nested algorithm of truss structure topology optimization can be done efficiently by a gradient-based mathematical programming algorithm. Through independent shakedown analysis and optimization processes, the proposed approach facilitates the computation scale and efficiency for large-scale structure optimization design. Several numerical examples involving two- and three-dimensional truss illustrate the effectiveness of the proposed approach.
ZmPRD1 is essential for double-strand break formation, but is not required for bipolar spindle assembly during maize meiosis
Authors: Wang Yazhong , Wang Yan , Zang Jie , Chen Huabang , He Yan 
Published: February 2022

Abstract
Homologs of PUTATIVE RECOMBINATION INITIATION DEFECT 1 (PRD1) are known to be essential for meiotic double-strand break (DSB) formation in mouse (Mus musculus), Arabidopsis, and rice (Oryza sativa). Recent research has shown that rice PRD1 also plays an unanticipated role in meiotic bipolar spindle assembly, revealing that PRD1 has multiple functions in plant meiosis. In this study, we characterize the meiotic function of PRD1 in maize (Zea mays; ZmPRD1). Our results show that Zmprd1 mutant plants display normal vegetative growth but have complete male and female sterility. Meiotic DSB formation is fully abolished in mutant meiocytes, leading to failure in homologous pairing, synapsis, and recombination. ZmPRD1 exhibits a different pattern of chromosome localization compared to its rice homologs. The ZmPRD1 protein interacts with several DSB-forming proteins, but does not directly interact with the kinetochore proteins REC8 and SGO1. Possibly as a result of this, there are no significant abnormalities of bipolar spindle assembly in Zmprd1 meiocytes. Overall, our results demonstrate that ZmPRD1 is essential for DSB formation and homologous recombination in maize meiosis. However, the recently-identified function of PRD1 in bipolar spindle assembly during rice meiosis is not conserved in maize.
A formalization of abstract argumentation in higher-order logic
Authors: Steen Alexander , Fuenmayor David 
Published: February 2022

Abstract
We present an approach for representing abstract argumentation frameworks based on an encoding into classical higher-order logic. This provides a uniform framework for computer-assisted assessment of abstract argumentation frameworks using interactive and automated reasoning tools. This enables the formal analysis and verification of meta-theoretical properties as well as the flexible generation of extensions and labellings with respect to well-known argumentation semantics.
Skills for Getting Started
Authors: Steffen Ann M. , Dick-Siskin Leah P. , Bilbrey Ann Choryan , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

Abstract
The research literature on preventing premature dropout from psychotherapy demonstrates that providers can do much to increase the likelihood that clients will remain in treatment long enough to benefit. Important therapeutic actions include clinicians explaining the therapy approach, setting expectations for treatment length, directly linking treatment approach to presenting problems, instilling hope, and asking for and responding to feedback to build the therapeutic alliance. This first personalized workbook module of Treating Later-Life Depression: A Cognitive Behavioral Therapy Approach supports the work by therapists with their middle-aged and older clients to begin treatment. Easy-to-read, large-print Learn pages are selected by practitioners to help aging clients develop positive expectations for treatment, understand depression, identify values and strengths, explore intersecting multicultural identities, personalize the CBT model, develop therapy goals, shape expectations for how change happens, and recognize the importance of home practice. Practice forms are provided for use between sessions to support this initial phase of treatment. The experience of psychotherapy is heavily shaped by social contexts and environments. Practitioners are encouraged to apply strategies that are responsive to the needs of culturally diverse aging clients.
Skills for Managing Chronic Pain
Authors: Steffen Ann M. , Dick-Siskin Leah P. , Bilbrey Ann Choryan , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

Abstract
There are a number of health conditions experienced by middle-aged and older adults that lead to persistent pain. Chronic pain and depression are bidirectionally related - chronic pain contributes to the development of later-life depression, and depression increases the difficulty of implementing pain management strategies. This personalized workbook module of Treating Later-Life Depression: A Cognitive Behavioral Therapy Approach promotes skills to manage chronic pain and support daily living that is consistent with personal values and life goals. Easy-to-read, large-print Learn pages are selected by behavioral health clinicians to help aging clients understand and apply the gate control theory of pain to regulate emotions, thoughts and behaviors that increase pain perceptions and disrupt value-based living. Together with their provider, clients choose specific Practice forms for use between sessions to practice, remember, and use these skills. The experience of chronic pain is embedded within social contexts and environments. Practitioners are encouraged to apply strategies that are responsive to the needs of culturally diverse aging clients.
Skills for Living with Loss
Authors: Steffen Ann M. , Dick-Siskin Leah P. , Bilbrey Ann Choryan , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

Abstract
Deaths become more common with increasing age, including the deaths of pets, close friends, parents, siblings, and life partners. Other important losses can be related to retirement, declining health (of the client or someone they care for), a move, and/or loss of roles/activities that have been important parts of personal identity for middle-aged and older adults. This personalized workbook module of Treating Later-Life Depression: A Cognitive Behavioral Therapy Approach provides flexible clinical tools for mental health interventions with aging clients who are grieving different types of loss, with or without significant depressive symptomatology. Easy-to-read, large-print Learn pages are selected by behavioral health clinicians to support the clinical tasks of being present to and normalizing common grief reactions. Practice forms are available to use between sessions to support clients’ grieving processes and management of daily life tasks. Grief reactions are embedded within social contexts and environments. Practitioners are encouraged to apply grief therapy strategies that are responsive to the needs of culturally diverse aging clients.
Psychotherapy Modifications with Aging Clients
Authors: Steffen Ann M. , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

AbstractAge-related adaptations to individual and group psychotherapy, as well as within integrated primary care practice, generally involve contextual modifications—in other words, the “how” of implementing specific change strategies. This chapter provides tips and strategies for providers conducting therapy sessions with depressed clients across the second half of life (i.e., individuals in their 50s, 60s, 70s, 80s, and 90s). Specific challenges that arise in working with some aging clients are discussed, followed by potential remedies, including within the context of telehealth. Practitioners are encouraged to apply recommended strategies that are responsive to the needs of culturally diverse aging clients.
Module 3: Skills for Doing: Values-Based Living and Solving Problems
Authors: Steffen Ann M. , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

AbstractConsiderable research done over the past 50 years has shown that as individuals become more actively engaged in daily life, depressive symptoms decrease. Thus, increasing daily rewarding, meaningful, and valued activities is a priority in treatment for depression. This chapter supports providers as they implement behavioral activation and problem-solving strategies consistent with clients’ personal values and strengths. The clinician’s focus should be on helping aging clients understand the role of activities in depression, engage in positive daily activities, and apply the steps of problem solving to anticipate and remove barriers. This chapter also describes the Practice forms provided for clients’ use between psychotherapy and integrated primary care sessions to remember and use these skills. This chapter directs practitioners to apply specific strategies that are responsive to the needs of culturally diverse aging clients, including within the context of telehealth.
Module 11: Skills for Wrapping Up: Finishing Treatment
Authors: Steffen Ann M. , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

AbstractThis chapter supports providers as they use flexible clinical tools for the psychotherapy termination process with middle-aged and older adults. Termination issues covered include the therapy relationship, review of skills learned, planning for future stressful situations, and recognizing signs of relapse. This chapter also describes the Practice forms that clients can use to develop a maintenance guide that supports daily living consistent with personal values and life goals after therapy has ended. Psychotherapy relationships and termination processes are embedded within social contexts and environments. This chapter directs practitioners to apply specific strategies that are responsive to the needs of culturally diverse aging clients, including within the context of telehealth.
Module 5: Skills for Brain Health: Healthy Cognitive Aging
Authors: Steffen Ann M. , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

AbstractThis chapter supports providers as they help middle-aged and older adults manage age-related changes in cognitive functioning and engage in daily living that is consistent with their personal values and life goals. This chapter describes the relevant Learn pages and Practice forms provided in the client workbook, focused on helping aging clients understand cognitive aging, engage in brain-healthy habits, navigate health care, manage medications, and consider whether to complete a cognitive evaluation. The experience of cognitive aging is embedded within social contexts and environments. This chapter directs clinicians to apply recommended strategies that are responsive to the needs of culturally diverse aging clients, including within the context of telehealth.
Module 9: Skills for Living with Loss: Bereavement and Grief
Authors: Steffen Ann M. , Thompson Larry W. , Gallagher-Thompson Dolores 
Published: February 2022

AbstractMiddle-aged and older adults experience the deaths of pets, friends, parents, siblings, and life partners. Other losses are related to retirement, declining health, moves, and loss of roles that have been important parts of personal identity. This chapter suggests clinical tools to support clients’ grieving processes and management of daily life while living with loss. Clinical tasks include being present to and normalizing common grief reactions. This chapter describes the Practice forms provided in the workbook for aging clients’ use between psychotherapy and integrated primary care sessions to remember and use these skills. Clinicians are encouraged to apply recommended grief therapy strategies that are responsive to the needs of culturally diverse aging clients, including within the context of telehealth.
Session 4: Developing a Master Plan
Authors: Weersing V. Robin , Gonzalez Araceli , Rozenman Michelle 
Published: February 2022

Abstract
Chapter 4 (Session 4) marks an important point in Brief Behavioral Therapy (BBT) because it sets the stage for rest of the sessions in BBT. In addition to reviewing skills from previous sessions, this session highlights avoidance as an unhelpful habit and helps youth understand how facing stressful situations head-on in a step-by-step way can be a helpful response that will ultimately decrease stress and negative emotions. This chapter will help youth select major treatment goals and begin developing the Master Plan with the help of the therapist. A Master Plan is a list of tasks that the youth will complete over time to help them face stressful situations. Before youths try these tasks on their own, they will practice together with the therapist.
The Final Session: Onward and Upward!
Authors: Weersing V. Robin , Gonzalez Araceli , Rozenman Michelle 
Published: February 2022

Abstract
Chapter 6 outlines the final session of Brief Behavioral Therapy (BBT), which reviews everything youth have accomplished in BBT, reviews the main ideas covered in BBT, and plans out ways to manage stress in the future. Specifically, youth will review skills they learned in BBT for coping with stress and daily hassles, plan long-term goals, establish healthy habits and social support, think about upcoming big changes, and discuss a plan in the event that difficulties arise in the future. The handouts and worksheets in this chapter are designed to help ensure that youth end BBT with the understanding that the most helpful way to respond to stress is to face the things that are difficult and that they can use the skills they’ve learned to tolerate negative feelings, solve problems, and make plans for action that involve taking small steps toward dealing with stress.
Brief Behavioral Therapy for Anxiety and Depression in Youth
Authors: Weersing V. Robin , Gonzalez Araceli , Rozenman Michelle 
Published: February 2022

Abstract
Taken together, anxiety and depression are the most common mental health problems across the lifespan, with most adults dating the onset of their struggles to childhood and adolescence. The Brief Behavioral Therapy (BBT) program for youth is a transdiagnostic intervention for anxiety and depression designed to efficiently treat the cluster of internalizing symptoms most commonly seen in childhood and adolescence: feeling stressed, sad, bad, nervous, worried, moody, irritable, or scared. BBT works by targeting core processes implicated in both anxiety and depression. In BBT, youth learn to reduce their avoidance of situations that produce negative feelings and increase their approach toward and engagement with sources of reward and meaning. Of note, the BBT model eliminates cognitive restructuring, a central ingredient in cognitive-behavioral therapies for depression and anxiety. BBT is intended to be briefer and less complex to learn and to teach as compared to alternate transdiagnostic and disorder-specific interventions for pediatric anxiety and depression. This volume is the Therapist Guide, designed to be used in combination with the BBT Workbook for youth and families. Each session in this guide builds on skills taught in the previous session, and each session has matching material in the Workbook. The Therapist Guide also includes supplemental material for working with less common clinical situations and therapist reflection exercises designed to mimic the supervision style of research studies using BBT. Overall, the BBT program has been found to significantly reduce symptoms and improve functioning in daily life for youths with anxiety and depression.
Sessions 5 Through 11: Implementing the Master Plan
Authors: Weersing V. Robin , Gonzalez Araceli , Rozenman Michelle 
Published: February 2022

Abstract
Chapter 5 describes implementation of the Master Plan that will begin Session 5 and continue for the remainder of treatment until the second to last session (typically anywhere from Session 8 to Session 11 depending on treatment progress). Each session primarily consists of in-session practice of items on the Master Plan with detailed planning for implementing the Master Plan between therapy sessions (and in the youth’s daily life). This chapter and the accompanying appendix provide material to guide the therapist, including sample Master Plans, suggestions for tailoring the Master Plan for different symptom presentations, tips for successful implementation and calibration of difficulty level, ongoing use of Problem-Solving PLANS to address challenges, guidance for parent involvement, and anticipation of treatment termination. Additionally, this chapter describes the Therapist Reflection Worksheet as a tool to help the therapist with treatment planning.
Final Session: Relapse Prevention
Authors: Weersing V. Robin , Gonzalez Araceli , Rozenman Michelle 
Published: February 2022

Abstract
Chapter 6 outlines the final session of BBT, which reviews progress and conducts relapse prevention. The therapist, youth, and parent will review skills acquired for coping with stress, symptoms, and negative life events. In addition, the therapist will celebrate progress toward treatment goals and highlight accomplishments while linking improvement to an increase in approach behavior. Relapse prevention involves discussion of long-term goals, addressing remaining symptoms that were not targeted in BBT, establishing healthy habits and social support, anticipating upcoming stressors, and discussing a plan in the event that difficulties arise in the future. This will help ensure that youth end BBT with the understanding that the most helpful way to respond to stress is to face the things that are difficult, that they can tolerate negative feelings, and that they can do the things they want and need to do in the future with these skills even when they feel stressed.