bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Metabolic Signatures of Surviving Cotwins in Cases of Single Intrauterine Fetal Death During Monochorionic Diamniotic Pregnancy: A Prospective Case-Control Study
Authors: Liu Xiyao , Fu Huijia , Wen Li , Zhu Fangyu , Wu Yue , Chen Zhi , Saffery Richard , Chen Chang , Qi Hongbo , Tong Chao , Baker Philip N. , Kilby Mark D. 
Published: April 2022

Introduction: Single intrauterine fetal death (sIUFD) in monochorionic diamniotic (MCDA) twin pregnancy may be associated with adverse clinical outcomes and possible metabolic changes in the surviving co-twin. Metabolomic profiling has not been undertaken before in these complex twin pregnancies.Methods: In this prospectively collected case-control study, three cross-cohort comparisons were made between sIUFD MCDA (n = 16), uncomplicated MCDA (n = 16, eight pairs), and uncomplicated singleton pregnancies (n = 8). To identify major sources of variation within the sIUFD MCDA cohort, a secondary comparison was conducted between spontaneous sIUFD (n = 8) and sIUFD in MCDA twins due to selective termination of a single abnormal fetus by radiofrequency ablation (RFA) (n = 8). Metabolomics analysis of placental tissue and umbilical cord plasma was performed using LC-MS profiling. The underlying metabolic networks and pathways were analyzed by web-based platforms. Associations and statistical correlations of all identified differential metabolites with neonatal birthweight and birth length were assessed by multivariable linear regression, adjusted for maternal age and gestation.Results: Across four comparisons, 131 and 111 differential metabolites were identified in placental tissue and cord plasma, respectively, with the highest variation seen between the spontaneous vs. single-induced IUFD in MCDA twins by RFA in the cord plasma. Conversely, the number of viable fetuses and the presence of sIUFD in MCDA twins had the highest impact on metabolite variation in placental tissue. Compounds correlated with fetal growth including placental acylcarnitines and gangliosides, along with specific amino acids (e.g., histidinyl-hydroxyproline), xenobiotics and biliverdin in cord plasma.Conclusion: sIUFD in MCDA twin pregnancy correlates with distinctive metabolic signatures, mostly in fatty acyls and complex lipids, in placental tissue and cord plasma of the surviving cotwin. Some metabolites are also associated with fetal growth.
CDK6 Immunophenotype Implicates Potential Therapeutic Application of CDK4/6 Inhibitors in Urothelial Carcinoma
Authors: Sun Ran , Wang Xuemei , Zhang Leichao , Gu Yu , Yang Shaojuan , Wang Liping , Wang Xueju 
Published: April 2022

BackgroundInfiltrating bladder urothelial carcinoma is the most common bladder malignancy with limited therapeutic options and poor prognosis. Identifying new therapeutic targets or strategies has important clinical significance. The data from public sources indicate poor prognosis in urothelial carcinoma cases with high CDK6 mRNA levels. Furthermore, studies have shown that CDK6 expression is elevated in urothelial carcinoma tissue compared to the surrounding urothelium, thus presenting a case for performing CDK4/6 inhibitor targeted research in urothelial carcinoma. However, a phase II trial showed that CDK4/6 inhibitors are not effective for advanced urothelial carcinoma, suggesting that case screening is important for targeted therapy.ObjectiveImmunohistochemistry (IHC) is simple and easy to perform and can be used to screen urothelial carcinoma cases with high CDK6 expression in clinical practice. The aim of this study was to determine the CDK6 expression threshold for positive cases.MethodsWe evaluated the correlation between the H-score of CDK6 protein expression and survival or CDK6 mRNA level using RNA sequencing. The effects of different CDK4/6 inhibitors were tested on bladder carcinoma cell lines with different CDK6 expression levels.ResultsThe H-score, which predicts poor prognosis and reflects a high CDK6 mRNA level, was determined as the selection criterion for positive cases. Furthermore, we found that urothelial carcinoma cell lines with higher CDK6 expression levels displayed greater sensitivity to CDK4/6 inhibitors than cells with lower expression levels.ConclusionsIHC staining for CDK6 protein in urothelial carcinoma is proposed as a promising screening platform for CDK4/6 inhibitor targeted therapy.
Progression Patterns and Post-Progression Survival in Recurred Intrahepatic Cholangiocarcinoma Patients: A Novel Prognostic Nomogram Based on Multicenter Cohorts
Authors: Zhao Chongyu , He Chaobin , Lu Jiawei , Huang Xin , Chen Cheng , Lin Xiaojun 
Published: April 2022

BackgroundThe post-progression survival (PPS) of recurred intrahepatic cholangiocarcinoma (iCCA) patients relates to the characteristics of tumor progression. Moreover, the prediction model of PPS in those patients has not been well established. This study aimed at developing a novel nomogram for predicting PPS in recurred iCCA patients.MethodClinical characteristics were retrospectively collected in 396 patients diagnosed with iCCA from cohorts of Sun Yat-sen University Cancer Center (SYSUCC) and the First Hospital of Dalian Medical University (FHDMU). The PPS in patients with different progression patterns was investigated. The nomogram of PPS was established with the Cox regression model in the primary cohort. Then the nomogram was verified in the external validation cohort.ResultsLiver progression was the commonest pattern (42.08%) in recurred iCCA patients, while patients with local LN progression had significantly better PPS than those with other patterns. The independent prognostic factors comprised elevated CEA levels, tumor differentiation, N stage 8th, adjuvant therapy, Local LN metastasis, Liver Metastasis only, and Multiple Metastasis. The nomogram constructed on these factors achieved satisfied C-indexes of 0.794 (95% CI 0.769–0.828) and 0.827 (0.779–0.876) for the training and validation cohorts, respectively. These values were significantly higher than those of the 8th TNM stage system (all p &lt; 0.001). The recurred iCCA patients could be precisely classified into high- and low-risk groups according to the cutoff point of this nomogram (p &lt; 0.01).ConclusionThe investigation of progression patterns and the development of this nomogram can offer new evidence to precisely postoperative and post-progression management of iCCA patients.
Term Neonatal Complications During the Second Localized COVID-19 Lockdown and Prolonged Premature Rupture of Membranes at Home Among Nulliparas With Reference Interval for Maternal C-Reactive Protein: A Retrospective Cohort Study
Authors: Geng Yang , Zhao Weihua , Liu Wenlan , Tang Jie , Zhang Hui , Ke Weilin , Yao Runsi , Xu Ji , Lin Qing , Li Yun , Huang Jianlin 
Published: April 2022

ObjectiveThe COVID-19 lockdown extended premature rupture of membranes (PROM) expectant time among nulliparas and increased the risk of term neonatal complications. This study investigated the impact of term nulliparas with PROM delays at home on neonatal outcomes during the COVID-19 lockdown period, considering the clinical diagnostic application of maternal C-reactive protein (CRP).MethodsThis study collected 505 term nulliparous women who underwent PROM at home from five provinces in a non-designated hospital of China in 2020. We analyzed PROM maternal information at home and neonatal complications in the COVID-19 regional lockdown and compared related information in the national lockdown. Poisson regression models estimated the correlation of PROM management at home, maternal CRP, and neonatal morbidity. We constructed two diagnostic models: the CRP univariate model, and an assessed cut-off value of CRP in the combined model (CRP with PROM waiting time at home).ResultsIn the regional lockdown, PROM latency at home and the severity of neonatal complications were extended and increased lower than in the nationwide lockdown, but term neonatal morbidity was not reduced in the COVID-19 localized lockdown. Prolonged waiting time at home (≥8.17 h) was associated with increasing maternal CRP values and neonatal morbidity (adjusted risk ratio 2.53, 95% CI, 1.43 to 4.50, p for trend &lt;0.001) in the regional lockdown period. In the combined model, CRP ≥7 mg/L with PROM latency ≥8.17 h at home showed higher diagnostic sensitivity and AUC than only CRP for initial assessing the risk of adverse neonatal complications in COVID-19 regional lockdowns (AUC, 0.714 vs. 0.534; sensitivity, 0.631 vs. 0.156).ConclusionThe impact of the acute COVID-19 national blockade on the PROM newborns' health could continue to the COVID-19 easing period. Maternal CRP reference interval (≥7 mg/L) would effectively assess the risk of term neonatal morbidity when nulliparas underwent prolonged PROM expectant at home (≥8.17 h) during the second COVID-19 lockdown.
Relationship Between Cognitive Appraisal of Control and Cardiac Vagal Regulation During an Unsupported Ski Crossing of Greenland
Authors: Trabucchi Pietro , Savoldelli Aldo , Mourot Laurent , Vacher Philippe , Pellegrini Barbara , Schena Federico 
Published: April 2022

PurposeThe aim of the present study was to investigate the relationships between Perceived Control (PC) and Heart rate variability (HRV) during a 27-day expedition, during which an unsupported crossing was made from the west coast to the east coast of Greenland (across the Ice Sheet); and that therefore a high PC represents a favourable factor for recovery and stress management.MethodsFour subjects participated in the study. PC was measured on alternated days in the evening at the end of the day, using the Pearlin Mastery Scale; and the next day, upon waking, heart rate using a wrist heart rate monitor and a chest strap. Together with the PC, the perceived effort was measured through the CR-100 Borg scale and each subject was asked to indicate the most emotionally significant event of the day.Time and frequency domain indices for heart rate variability were calculated.ResultsSeveral correlations were observed between PC and HRV indices. In particular two indices in the time domain, standard deviation of all NN intervals (SDNN) (rrm = 0.51) and root mean square of successive (RMSSD) (rrm = 0.46), showed a significant and strong positive correlation.ConclusionThe existence of a positive correlation between PC and cardiac vagal regulation is of great interest to individuals immerged into extreme situations, because it can affect performance or prevents maladaptive states or injuries. To improve stress management, it could be convenient for members of extreme expeditions to adopt forms of cognitive training that modify their cognitive appraisal in order to raise their perception of control.
Efficacy and Safety of Novel Beta-Chitin Patches as Haemostat in Rat Vascular and Neurosurgical Model
Authors: Sabab Ahad , Vediappan Rajan Sundaresan , Finnie John , McAdam C. John , Jukes Alistair , Vreugde Sarah , Wormald Peter-John 
Published: April 2022

BackgroundIntraoperative hemorrhage is a major cause of poor post-operative outcome. Beta-chitin patch has previously been found to be an effective haemostat, but whether modifying the patch can improve its efficacy and safety, remains unknown. In this study, beta-chitin patches were modified using polyethylene oxide, Pluronic-F127 (Chi/F127), calcium (Chi/20%Ca), increased thickness (Chi/Thick) or polyphosphate (Chi/PP).ObjectiveUsing rat (Wistar Albino; 8–10 weeks old) vascular and neurosurgical models, this project investigated and compared the efficacy and safety of beta-chitin patches with gauze, Surgicel and FloSeal.MethodsNinety rats underwent a standardized femoral artery injury and were randomized to receive either beta-chitin patches, gauze, Surgicel or FloSeal. The bleeding time and total blood loss was measured. For the neurosurgical model, forty-four rats underwent a standardized cortical injury and randomization to a treatment group. Following a 48 h recovery period, their brains were collected for histopathological examination.ResultsThe mean bleeding time with Chitin (120.8 s) and Chi/PP (117.3 s) was ~60 s lower than Chi/F127, Chi/20%Ca and Chi/Thick (p &lt; 0.05). Chitin and Chi/PP had a significantly lower bleeding time than FloSeal (174.2 s) (p &lt; 0.05), but not Surgicel (172.7 s). Gauze (400 s) had a significantly higher bleeding time compared to all other groups (p &lt; 0.05). There were no significant differences in the total blood loss between the groups. Histopathological examination of brains found no adverse inflammatory reaction to any of the haemostatic compounds.ConclusionChi/PP had superior haemostatic efficacy compared to Surgicel and FloSeal, but not compared to non-modified beta-chitin patch. All of the haemostats were equally safe.
CARACTERIZACIÓN MORFOLÓGICA Y AGRONÓMICA DE GERMOPLASMA DE TOMATE NATIVO DEL SUR DE MÉXICO
Authors: Canul-Ku Jaime , González-Pérez Enrique , Barrios-Gómez Edwin Javier , Pons-Hernández José Luis , Rangel-Estrada Sandra Eloísa 
Published: April 2022

Para establecer un programa de mejoramiento genético de tomate (Solanum lycopersicum L.) se requiere tener acceso a germoplasma que contenga genes de interés comercial y valor nutricional; previamente, se deben conocer las características y atributos de cada población, lo cual se logra con la caracterización. Con base en lo anterior, el objetivo de este estudio fue evaluar la diversidad morfológica y agronómica de 20 accesiones de tomate nativo del sur de México e identificar aquellas promisorias para conservarlas y usarlas en mejoramiento genético. Las poblaciones se establecieron bajo un diseño experimental completamente al azar con tres repeticiones. Se registraron 18 caracteres de planta, flor, fruto y fenología. La información obtenida se sometió a análisis de varianza, de componentes principales y de conglomerados. El 82.3 % de la variación morfo-agronómica total se explicó con los tres primeros componentes principales. Los caracteres con mayor valor descriptivo de la variabilidad fueron estructuras florales, tamaño y peso de fruto, rendimiento por planta, longitud de nudos, altura de la planta hasta el primer racimo y precocidad. El análisis de conglomerados y de componentes principales permitió la identificación de cinco grupos. Se identificaron seis poblaciones sobresalientes con los mayores promedios en estructura de la flor, largo de fruto, peso de fruto y rendimiento de fruto por planta.
RENDIMIENTO E ÍNDICE DE COSECHA DE GERMOPLASMA DE FRIJOL PINTO Y FLOR DE MAYO BAJO ESTRÉS POR TEMPERATURA ALTA
Authors: Anaya-López José Luis , Rojas-Tovar Luis Manuel , Cisneros-López Hugo César , Acosta-Gallegos Jorge Alberto 
Published: April 2022

La productividad del frijol de riego sembrado en febrero en la región de El Bajío de Guanajuato, México es afectada por temperaturas altas durante la etapa reproductiva. El objetivo de este estudio fue determinar el efecto de la temperatura alta sobre el rendimiento e índice de cosecha en germoplasma mejorado de frijol de los tipos Pinto y Flor de Mayo e identificar materiales con rendimiento alto en riego. En 2018 se evaluaron 16 materiales de frijol Pinto y 15 de Flor de Mayo. Durante el ciclo se aplicó un riego de presiembra y tres de auxilio. Cada material se sembró en dos surcos de 30 m, y a la cosecha se tomaron al azar seis tramos de 1 m para determinar el número de vainas, numero de granos, producción de biomasa, rendimiento de grano, peso de cien semillas e índice de cosecha (IC). Los grupos se analizaron por separado bajo un diseño completamente al azar y prueba de medias dentro de grupos (Tukey, P ≤ 0.05). Durante la etapa reproductiva las temperaturas diurna y nocturna máximas superaron los 30 y 20 oC, respectivamente. En ambos grupos hubo diferencias significativas (P ≤ 0.05) en las características evaluadas. El rendimiento mayor en los materiales de tipo Pinto lo tuvo la variedad Salinas (3.79 t ha-1) y en los de tipo Flor de Mayo la línea (PS/FM Dolores)-M-9-2-9 (3.27 t ha-1), ambos mostraron número alto de vainas por m2 y granos por vaina. En general, en ambos grupos el IC resultó bajo. En los materiales de tipo Pinto la correlación entre IC y rendimiento fue positiva (r = 0.75**), mientras que en el grupo Flor de Mayo la correlación del IC con la biomasa fue negativa (r = -0.74**), lo que mostró la importancia de la capacidad de movilización de fotoasimilados hacia la semilla en condiciones de estrés por calor y la superioridad de los genotipos Pintos.
Environmental aspects of the production and use of autoclaved aerated concrete with low density
Authors: Rudchenko Dmitry , Serdyuk Vasyl 
Published: April 2022

The purpose of the article is to investigate the state of carbon dioxide emissions generated as a result of wall materials production and the construction industry as a whole. The paper provides a comparative analysis of housing construction in Ukraine and in some CIS countries. Against the background of the indicators of low housing availability and low relative volumes of housing construction, the issue of high energy consumption remains valid. The absolute and relative volumes of greenhouse gas emissions from economic activity of the population of Ukraine are given. The projected consequences for Ukraine from continued CO2 emissions on the same level and temperature rise are estimated. It is deemed that the most suitable wall material for countries with emerging economies is autoclaved aerated concrete (AAC); since it is a structural and thermal insulating material, it can solve the problem of housing construction.
The paper shows the key priority of low energy consumption and makes emphasis on lowering the density of the produced aerated concrete, reducing mineral additives and cement production expenses.
PEMETAAN JALUR EVAKUASI GUNUNG MERAPI DEMPO KOTA PAGAR ALAM MENGGUNAKAN APLIKASI SISTEM INFORMASI GEOGRAFIS
Authors: Ariansa  , Dhiniati Fameira , Endah Diansari Lily 
Published: April 2022

Natural disasters are one of the phenomena that can occur at any time, anywhere and at any time, causing risks to human life, both property losses and human lives. Based on the data, the purpose of the study is the mapping of the evacuation route of Mount Merapi Dempo using the GIS Application. The method used Network analysis (Network Analysis) with the Model Find the best facilities This form of network analysis combines the network and physical facilities Routes This form complements the convenience of connecting networks by providing solutions to determine the best routes that must be passed. And Find the nearest facility of this network analysis between the network and other facilities to get services that are faster or easier to reach. The results of the evacuation route by class, Find the nearest Facilities and Find the Best Route as for the route that goes to the class Find the closeset Facilities Are Kampung 4, Janang, Talang beduk, Bedeng Krese, Ptpn 7, Rimau, Tangsi II, enter the class route Find the Best Route.
PELATIHAN PENJERNIH AIR SEDERHANA UNTUK KEPERLUAN MENCUCI TANGAN GUNA PENCEGAHAN COVID-19 DI DUSUN SUKAJADI KOTA PAGAR ALAM
Authors: Diansari Lily Endah , Gusmaliza Debi 
Published: April 2022

Dusun Sukajadi Kota Pagar Alam, Provinsi Sumatera Selatan berada pada jalur lintas provinsi sehingga aktivitas mobilisasi yang tinggi pada jalur ini lebih berisiko dalam penyebaran covid-19. Sumber air di Dusun Sukajadi adalah air tanah dengan kondisi yang sering berwarna atau keruh. Kondisi air yang keruh tidak memenuhi syarat kesehatan. Salah satu teknologi tepat guna yang dapat meningkatkan mutu kesehatan dan pencegahan covid-19 pada masyarakat Dusun Sukajadi yang lingkungan alaminya kurang tersedia air bersih adalah dengan pembuatan konstruksi penjernih air sederhana. Tujuan dari Pengabdian kepada Masyarakat ini adalah untuk meningkatkan pengetahuan mitra tentang penjernih air dan meningkatkan keterampilan membuat konstruksi penjernih air. Manfaat dari kegiatan ini adalah bagi masyarakat Dusun Sukajadi dapat memahami tentang konstruksi penjernih air dan meningkatnya keterampilan masyarakat dalam membuat penjernih air. Metode yang digunakan pada kegiatan ini adalah memberikan pengetahuan tentang bahaya covid-19 dan cara pencegahannya kemudian penyuluhan tentang penjernih air dan melakukan pelatihan membuat penjernih air dengan ceramah, tanya-jawab, diskusi dan praktik. Hasil yang dicapai dalam kegiatan PkM ini adalah minat dan motivasi mitra cukup tinggi dalam berkontribusi dalam kegiatan berupa pelatihan yang diberikan oleh tim pelaksana dan peserta memperoleh pengetahuan dan keterampilan tentang penjernih air. Hasil yang dicapai dalam kegiatan ini juga meningkatkan pengetahuan peserta tentang bahaya Covid-19 dan upaya-upaya dalam pencegahan penyebaran Covid-19.
PENERAPAN APLIKASI E-SURAT PADA KANTOR PERBEKEL DESA KUKUH KECAMATAN KERAMBITAN, TABANAN
Authors: Susila Satwika I Kadek , I Putu Susila Handika  , I Nyoman Buda Hartawan  
Published: April 2022

Pengelolaan surat khususnya pada instansi kedinasan merupakan hal yang penting untuk dilakukan. Karena segala bentuk pencataan kegiatan berada pada arsip surat masuk maupun surat keluar. Namun selama ini proses pencataan surat pada Desa Kukuh Kecamatan Kerambitan, Tabanan masih dilakukan secara manual. Sehingga proses dari awal surat masuk sampai surat di disposisi membutuhkan waktu yang lama. Berdasarkan permasalah tersebut, kebutuhan yang diperlukan untuk menunjang proses bisnis pengelolaan surat masuk, mekanisme disposisi, serta pembuatan laporan kegiatan adalah membangun sebuah sistem informasi E-Surat. Setelah dibangun sistem informasi E-Surat yang mampu mengelola surat masuk, mekanisme disposisi, serta pembuatan laporan, dilakukan pembinaan kepada SDM pada Pemerintahan Desa Kukuh untuk mengelola dan mengoptimalkan sistem E-Surat. Dengan adanya aplikasi E-Surat, permasalahan yang dialami oleh perangkat Desa Kukuh dapat diatasi. Dimana proses manajemen surat dapat dilakukan dengan lebih mudah, dan proses disposisi menjadi lebih cepat. Dari hasil jejak pendapat yang dilakukan bahwa 100% sudah bisa mengunakan aplikasi E-Surat, namun 75% (6 dari 8 staff Desa) yakin aplikasi E-Surat dapat membantu proses pengelolaan surat.
Pelatihan Troubleshooting Computer Pada Perangkat Kelurahan Dalam Peningkatan Pelayanan Prima Terhadap Masyarakat
Authors: Asep Syaputra  
Published: April 2022

Perkembangan teknologi berdampak pada pembentukan kondisi untuk sistem yang baik. Perusahaan publik dan perusahaan swasta berlomba-lomba menciptakan lingkungan kerja dan persaingan memperebutkan SDM yang dapat menggunakan teknologi untuk meningkatkan produktivitas pekerjaan. Saat menggunakan teknologi, bisa dipastikan akan ada masalah saat digunakan Perangkat keras atau perangkat lunak. Kelurahan Karang Dalo memiliki 13 perangkat Kelurahan, diantara semua perangkat Kelurahan yang ada, hanya sedikit orang yang dapat mengoperasikan dan mengatasi troubleshooting komputer. Situasi ini secara tidak langsung dampaknya pada proses pelayanan tidak maksimal. Dengan adanya permasalahan tersebut hingga perlu diselenggarakan pelatihan Troubleshoothing computer baik perangkat keras ataupun perangkat lunak bagi pegawai Kelurahan untuk meningkatkan kemampuan pengoperasian komputer khususnya pada Kelurahan Karang Dalo Kecamatan Dempo Tengah Kota Pagar Alam.