bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanism of repression of the glycerol utilization operon inBorrelia burgdorferi
Authors: Zhang Jun-Jie , Ranghunandanan Sajith , Wang Qian , Lou Yongliang , Yang X. Frank 
Published: November 2022

ABSTRACTLyme disease pathogenBorrelia burgdorferi, switches carbohydrate utilization while transmitting between two disparate hosts, anIxodestick vector and a mammalian host. The ability to use glycerol is important forB. burgdorferito colonize and persist in the tick vector, and expression of theglpFKD(glp) operon for glycerol uptake/utilization is tightly regulated during the enzootic cycle ofB. burgdorferibetween ticks and mammals. In present study, we identified aciselement within the 5’ untranslated region ofglpthat negatively regulates theglpexpression. This repression ofglpis independent of RpoS. We further show that BadR directly binds to thisciselement and repressesglp. The efficiency of BadR binding in the presence of c-di-GMP and various carbohydrates were also assessed. This finding, along with previous findings of positive regulation ofglpexpression by the c-di-GMP signaling pathway and negative regulation by the alternative sigma factor RpoS, demonstrates thatB. burgdorferiemploys multi-layer regulatory mechanisms to coordinateglpexpression during its enzootic cycle.
A Checking Model of Obsessive-Compulsive Disorder Based on the Hypothalamic Defensive System Against a Potential Threat
Authors: Horii-Hayashi Noriko , Masuda Kazuya , Kato Taika , Kobayashi Kenta , Nishi Mayumi 
Published: November 2022

ABSTRACTBACKGROUNDObsessive-compulsive disorder (OCD) is a psychiatric disorder characterized by obsessive thoughts and compulsive behavior. While some theories imply that OCD patients have cognitive biases and dysfunctional motivation regarding a potential threat, these views are not adequately supported by neurological evidence. Hypothalamic perifornical (PeF) urocortin-3 (UCN3) neurons are involved in defensive responses to a potential threat, and the activation of these neurons in mice induces repetitive and excessive checking and burying of novel objects. In this study, we evaluated the hypothesis that mice in which PeF UCN3 neurons are activated can serve as an OCD model.METHODSPeF UCN3 neurons were chemogenetically activated with clozapine-N oxide (CNO) inUcn3-Cremice. Marble-burying activity, repetitive/stereotypic behaviors in the homecage, and excessive responses to a novel object were measured as OCD-like behaviors. The effects of clinically used drugs for treating OCD on these behaviors were evaluated. The effect of CNO on neural activity in the cortico-striato-thalamo-cortical loop (which is regarded as an OCD circuit) was assessed with c-Fos immunolabeling.RESULTSCNO increased marble-burying activity, evoked homecage-specific repetitive/stereotypic behaviors that probably aimed to seal entrances, and induced repetitive and excessive checking and burying of novel objects. These behaviors were suppressed by selective serotonin-reuptake inhibitors but not by diazepam. CNO increased neural activity in the cortico-striato-thalamo-cortical loop.CONCLUSIONSThese results indicated that mice whose PeF UCN3 neurons are activated can serve as a model of OCD, particularly as a checking model. This supports theories concerning the role of potential threats in the pathophysiology of OCD.
Structural insights into 3Fe-4S ferredoxins diversity inM.tuberculosishighlighted by a first redox complex with P450
Authors: Gilep Andrei , Varaksa Tatsiana , Bukhdruker Sergey , Kavaleuski Anton , Ryzhykau Yury , Smolskaya Sviatlana , Sushko Tatsiana , Tsumoto Kouhei , Grabovec Irina , Kapranov Ivan , Okhrimenko Ivan , Marin Egor , Shevtsov Mikhail , Mishin Alexey , Kovalev Kirill , Kuklin Alexander , Gordeliy Valentin , Kaluzhskiy Leonid , Gnedenko Oksana , Yablokov Evgeniy , Ivanov Alexis , Borshchevskiy Valentin , Strushkevich Natallia 
Published: November 2022

AbstractFerredoxins are small iron-sulfur proteins and key players in essential metabolic pathways. Among all types, 3Fe-4S ferredoxins are less studied mostly due to anaerobic requirements. Their complexes withcytochromeP450 redox partners have not been structurally characterized. In the present work, we solved the structures of both 3Fe-4S ferredoxins fromM. tuberculosis- Fdx alone and the fusion FdxE–CYP143. Our SPR analysis demonstrated a high affinity binding of FdxE to CYP143. According to SAXS data, the same complex is present in solution. The structure reveals extended multipoint interactions and the shape/charge complementarity of redox partners. Furthermore, FdxE binding induced conformational changes in CYP143 as evident from the solved CYP143 structure alone. The comparison of FdxE–CYP143 and modeled Fdx–CYP51 complexes further revealed the specificity of ferredoxins. Our results illuminate the diversity of electron transfer complexes for the production of different secondary metabolites.
The IgCAM CAR regulates gap junction mediated coupling on embryonic cardiomyocytes and affects their beating frequency
Authors: Matthaeus Claudia , Jüttner René , Gotthardt Michael , Rathjen Fritz G. 
Published: November 2022

AbstractThe IgCAM coxsackie-adenovirus receptor (CAR) is essential for embryonic heart development and for electrical conduction in the mature heart. However, it is not well-understood how CAR exerts these effects at the cellular level. To address this question, we analysed the spontaneous beating of cultured embryonic hearts and cardiomyocytes from wildtype and CAR knockout (KO) embryos. Surprisingly, in the absence of CAR, cultured cardiomyocytes showed an increased frequency of beating and calcium cycling. Increased beating of heart organ cultures was also induced by application of reagents that bind to the extracellular region of CAR, such as the adenovirus fiber knob. However, the calcium cycling machinery, including calcium extrusion via SERCA2 and NCX, was not disrupted in CAR KO cells. In contrast, CAR KO cardiomyocytes displayed an increase in the size, but decrease in total number, of membrane-localized C×43 clusters. This was accompanied by improved cell-cell coupling between CAR KO cells, as demonstrated by increased intercellular dye diffusion. Our data indicate that CAR may modulate the localization and oligomerization of C×43 at the plasma membrane, which could in turn influence electrical propagation between cardiomyocytes via gap junctions.Simple summaryCAR is implicated in higher-order gap junction structure by regulating clustering of connexins in embryonic cardiomyocytes. In the absence of CAR, cardiomyocytes in culture showed an increased frequency of beating and calcium cycling. Perturbation experiments suggest that CAR could be a potential new candidate in cardiac diseases.
A clustering approach for data quality results of research information systems
Authors: Edris Abadi Reza , Ershadi Mohammad Javad , Niaki Seyed Taghi Akhavan 
Published: November 2022

Purpose
The overall goal of the data mining process is to extract information from an extensive data set and make it understandable for further use. When working with large volumes of unstructured data in research information systems, it is necessary to divide the information into logical groupings after examining their quality before attempting to analyze it. On the other hand, data quality results are valuable resources for defining quality excellence programs of any information system. Hence, the purpose of this study is to discover and extract knowledge to evaluate and improve data quality in research information systems.

Design/methodology/approach
Clustering in data analysis and exploiting the outputs allows practitioners to gain an in-depth and extensive look at their information to form some logical structures based on what they have found. In this study, data extracted from an information system are used in the first stage. Then, the data quality results are classified into an organized structure based on data quality dimension standards. Next, clustering algorithms (K-Means), density-based clustering (density-based spatial clustering of applications with noise [DBSCAN]) and hierarchical clustering (balanced iterative reducing and clustering using hierarchies [BIRCH]) are applied to compare and find the most appropriate clustering algorithms in the research information system.

Findings
This paper showed that quality control results of an information system could be categorized through well-known data quality dimensions, including precision, accuracy, completeness, consistency, reputation and timeliness. Furthermore, among different well-known clustering approaches, the BIRCH algorithm of hierarchical clustering methods performs better in data clustering and gives the highest silhouette coefficient value. Next in line is the DBSCAN method, which performs better than the K-Means method.

Research limitations/implications
In the data quality assessment process, the discrepancies identified and the lack of proper classification for inconsistent data have led to unstructured reports, making the statistical analysis of qualitative metadata problems difficult and thus impossible to root out the observed errors. Therefore, in this study, the evaluation results of data quality have been categorized into various data quality dimensions, based on which multiple analyses have been performed in the form of data mining methods.

Originality/value
Although several pieces of research have been conducted to assess data quality results of research information systems, knowledge extraction from obtained data quality scores is a crucial work that has rarely been studied in the literature. Besides, clustering in data quality analysis and exploiting the outputs allows practitioners to gain an in-depth and extensive look at their information to form some logical structures based on what they have found.
Construction and validation of a perceived financial well-being scale (PFWBS)
Authors: Vieira Kelmara Mendes , Matheis Taiane Keila , Bressan Aureliano Angel , Potrich Ani Caroline Grigion , Klein Leander Luiz , Rosenblum Tamara Otilia Amaral 
Published: November 2022

PurposeThe aim of the study is to build and validate a perceived financial well-being scale (PFWBS).Design/methodology/approachA total of 34 items were developed on a five-point Likert scale. Validation involved two phases and four steps. In the qualitative phase, interviews, validation by specialists and the pre-test were carried out. In the quantitative phase, a sample of 1,020 cases was used in the exploratory stage and another sample of 2,293 individuals in the confirmatory validation stage.FindingsThe PFWBS is composed of 23 items distributed in four dimensions (financial security, financial tranquility, financial freedom and satisfaction with financial management) that identify the perception of financial well-being of the consumers of financial products.Practical implicationsThe authors propose a methodological framework that allows researchers, managers and policy makers to use the indicator to assess citizens' perception of financial well-being.Social implicationsThe PFWBS can be useful in evaluating the results of different public policies, such as income transfer programs and financial education policies. It can also serve as a parameter for the financial system to assess the perception of its customers, helping to evaluate products and services.Originality/valueFinancial well-being lacks valid measurement scales in the literature. This study advances by creating a scale for the assessment of the perception of financial well-being, which can be applied in different contexts.
Smallholder farmers’ perceptions and adaptation strategies to climate change risks in northwest Ethiopia
Authors: Likinaw Aimro , Bewket Woldeamlak , Alemayehu Aragaw 
Published: November 2022

Purpose
The purpose of this paper was to examine smallholder farmers’ perceptions of climate change risks, adaptation responses and the links between adaptation strategies and perceived/experienced climate change risks in South Gondar, Ethiopia.

Design/methodology/approach
This paper used a convergent mixed methods design, which enables us to concurrently collect quantitative and qualitative data. Survey data was collected from 352 households, stratified into Lay Gayint 138 (39%), Tach Gayint 117 (33%) and Simada district 97 (28%). A four-point Likert scale was used to produce a standardised risk perception index for 14 climate events. Moreover, using a one-way analysis of variance, statistical differences in selecting adaptation strategies between the three districts were measured. A post hoc analysis was also carried out to identify the source of the variation. The findings of this paper are supplemented by qualitative data gathered through focus group discussions and key informant interviews of households who were chosen at random.

Findings
The standardised climate change risk perception index suggests that persistent drought, delayed onset of rainfall, early termination of rainfall and food insecurity were the major potentially dangerous climate change risks perceived by households in the study area. In response to climate change risks, households used several adaptation strategies such as adjusting crop planting dates, crop diversification, terracing, tree planting, cultivating drought-tolerant crop varieties and off-farm activities. A Tukey’s post hoc test revealed a significant difference in off-farm activities, crop diversification and planting drought-tolerant crop types among the adaptation strategies in the study area between Lay Gayint and Simada districts (p < 0.05). This difference reconfirms that adaptation strategies are location-specific.

Originality/value
Although many studies are available on coping and adaptation strategies to climate change, this paper is one of the few studies focusing on the linkages between climate change risk perceptions and adaptation responses of households in the study area. The findings of this paper could be helpful for policymakers and development practitioners in designing locally specific, actual adaptation options that shape adaptation to recent and future climate change risks.
Unethical pro-organizational behavior: how employee ethical ideology and unethical organizational culture contribute
Authors: Vem Linus Jonathan , Cheah Jun-Hwa , Ng Siew Imm , Ho Jo Ann 
Published: November 2022

PurposeThe corporate atmosphere in recent times speaks volumes about the crises of confidence and credibility brewing among professionals due to the rising incidences of unethical pro-organizational behavior (UPB). The study developed a model to demonstrate the underlying mechanisms through which unethical organizational culture (UOC) influences UPB through the mediating roles of idealism and relativism.Design/methodology/approachUsing a cross-sectional approach, data were collected through questionnaires that were distributed to small and medium-sized enterprises (SMEs) operating in the Plateau state in Nigeria. A total of 269 responses were obtained and analyzed using the partial least squares structural equation modeling (PLS-SEM) technique via Smart-PLS software.FindingsThe results revealed that the relationship between UOC and UPB was significant. The indirect predictive role of UOC on UPB was established via relativism but not through idealism. The results indicate that the preponderance of UPB among SMEs is a product of UOC which breeds a relativist ideology that ultimately promotes UPB. Finally, implications and suggestions for further research are discussed.Originality/valueThis study contributes to UPB in two unique ways. First, the authors bring to the fore the critical role of UOC in the debate on UPB which has been under-explored. Second, the study also established the mediating role of relativism in the relationship between UOC and UPB.
Social media and international business: views and conceptual framing
Authors: Saari Matti , Haapanen Lauri , Hurmelinna-Laukkanen Pia 
Published: November 2022

PurposeThe objective of this paper is to increase understanding of social media in international business context. To this end, the authors make an attempt to integrate the existing, still somewhat limited views in a framework that advances the knowledge of scholars and decision-makers on this topic.Design/methodology/approachThe authors conduct a conceptual study supported by use of a systematic literature review method.FindingsThis study shows marketing as a dominant area of discussion and reveals that many firm functions where social media plays a role have received relatively little attention. Furthermore, the study shows that the positive features of social media in international activity tend to be more widely acknowledged and better understood than the potentially problematic aspects.Research limitations/implicationsThe number of articles analyzed in this study was relatively small, resonating with the nature of an emerging research area. Research on social media has only taken off over the last years, and it is understandable that there is limited research that connects it specifically to phenomena of international business.Practical implicationsThis study reminds managers to be cautious when using social media in international markets. The relationship between social media and international business exhibits dynamism and is dependent on a variety of factors. Social media does not come without costs, nor is easily transferred from one market to another. Efficient use of this media in the international context may increase the need of specific and qualified human resources, and it may necessitate having the whole process from R&D to delivery, and beyond, ready for adaptation.Originality/valueIt can be argued that we know too little about the relevant factors and relationships between social media and international business. The authors hope that this study revealing the scarcely studied aspects and suggesting a tentative framework for capturing the dynamics of social media and international business can guide subsequent research and accelerate its emergence.
Identifying the capacity needs of built environment professionals to deliver environmentally sustainable buildings: a scientometric review
Authors: Afful Aba Essanowa , Ayarkwa Joshua , Acquah Godwin Kojo Kumi , Ossei Assibey Ama Antwi Darkwa , Osei-Asibey Dickson 
Published: November 2022

Purpose
The purpose of this study is to identify the capacity needs of building professionals to deliver environmentally sustainable buildings (ESBs) globally, from a unique systems approach. Through a review of extant literature, this study contributes to knowledge of the global delivery of ESBs by identifying research trends and gaps that can be tackled in future research, and current hotspots in capacity building (CB) research within the built environment (BE). The adopted systems approach to CB postulates that the construction industry is systemized in nature, and thus, CB solutions within the industry should be approached from a systems approach.

Design/methodology/approach
A literature search was conducted using the Scopus search engine, augmented by Google Scholar and Web of Science, to produce 54 relevant articles for analysis. The scientometric analysis was undertaken with the use of VOSViewer to better understand the broad literature on CB in the construction industry which would not have been possible with traditional data analysis. The content analysis allowed, for a systematic review of selected articles, to reveal key themes in this study.

Findings
Through a content analysis, four levels of CB were identified within the construction industry; individual level, organizational level, industry level and state level. Nine sub-capacities were subsumed under the four identified levels adopted from Potter and Brough (2004), including but not limited to Performance capacity, Workload capacity, Supervisory capacity, Industry Role capacity and Systems capacity. Under each sub-capacity, key questions were posed to aid identify the capacity needs of BE professionals. A framework for identifying capacity needs in the BE is proposed.

Practical implications
The findings of this study serve as a useful reference for practitioners and policymakers to assess their level of commitment to CB efforts for ESB delivery. The findings of this study have revealed that building the capacities of BE professionals to deliver ESBs should be addressed as part of a broader framework, interdependent on the other levels of CB in the systemic construction industry.

Originality/value
As a review study identifying capacity needs for BE professionals to deliver ESBs, this study enhances knowledge of CB within the construction industry.
Reinvesting in equity crowdfunding: the case of digital workers
Authors: Makki Rayan Faisal A. , Van Hemmen Stefan 
Published: November 2022

PurposeThe purpose of this study is to investigate the initial investment's motivations and study the reinvesting motivations. The results revealed differences in reinvestors' motivations of reinvestors in both winning and losing situations. Specifically, financial return and excitement motives were supported for win and loss situations, while recognition was supported for loss and pleasure in win situations.Design/methodology/approachThe impact of intrinsic and extrinsic motivations on reinvestors was tested using the structural equation model. Furthermore, the framework was analysed with survey data from a total of 355 digital workers from Amazon Mechanical Turk, one of the world's largest crowdsourcing platforms.FindingsThe results indicate that there are differences in the motivations for reinvestors when they are in both winning and losing situations. Financial return and excitement motives were supported for win and loss situation, while recognition was supported in loss and pleasure in win situation.Research limitations/implicationsThis study makes it possible to better understand the motivations behind crowdfunding reinvestment among digital workers. To build on this work, more studies should be conducted with different samples to test the generalisability of these results. Moreover, future studies on different samples could determine whether the same motivations would hold for other investors or whether another motivation would have greater impact on these reinvestment decisions.Originality/valueWhile previous research on equity crowdfunding has predominantly focused on intrinsic and extrinsic motivations for participating and investing in equity crowdfunding platforms, the motives that specifically affect winning or losing situations for reinvestors have been largely overlooked.