bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis and characterization of some new Schiff base derivatives of gabapentin, and assessment of their antibacterial, antioxidant and anticonvulsant activities
Authors: Saleem Muhammad Farrukh , Khan Mohsin Abbas , Ahmad Irshad , Aslam Naveed , Khurshid Umair 
Published: January 2022

Purpose: To synthesize and characterize some new gabapentin Schiff base derivatives, and to assess their antibacterial, antioxidant and antiepileptic activities.Methods: Four Schiff base derivatives of gabapentin, termed G1, G2, G3 and G4, were synthesized by condensation with benzoin, vanillin, acetophenone, and benzophenone, respectively. Their chemical identities were established by FTIR, 1 H NMR and 13C NMR techniques. The new compounds were screened for antibacterial activity using agar well method, antioxidant activity by DPPH assay, and anticonvulsant activity against pentylenetetrazole (PTZ) induced seizures in mice.Results: All the compounds showed antibacterial activity against the test strains to variable degrees, while the parent drug did not exhibit antibacterial activity. The zones of inhibition of compound G2 against Micrococcus luteus (36.2 ± 1.0 mm) and Serratia marcescens (28.2 ± 1.0 mm), and of compound G4 against Stenotrophomonas maltophilia (36.8 ± 1.0 mm) were larger compared to thestandard drug, doxycycline, exhibiting zones of inhibition 28.2 ± 1.3, 28.2 ± 0.9 and 20.0 ± 0.9 mm, respectively. In addition, compounds G1 and G2 possessed significantly greater (p < 0.05) radical scavenging activity (82.3 ± 1.8 and 92.3 ± 2.2 %, respectively) than the precursor drug, gabapentin (63.2± 2.6 %). The seizure scores for compounds G1 (0.7 ± 0.06) and G2 (0.9 ± 0.07) were comparable(p ˃ 0.05) with gabapentin (0.8 ± 0.06), while compounds G3 and G4 were less active (p < 0.05) than gabapentin.Conclusion: Compounds G1 and G2 exhibit good antibacterial and antioxidant activities while retaining the anticonvulsant activity of the parent drug, gabapentin, thus making them suitable candidates for further development for the treatment of neurodegenerative pathologies associated with bacterial infections.
Keywords: Gabapentin, Antibacterial, Seizures, Antioxidant, Anticonvulsant
Relationship between adherence to anti-diabetic medication and depression among patients with diabetes mellitus in three selected Chinese hospitals
Authors: Luo Haidong , Lin Yin , Li Jia , Xu Weiguo 
Published: January 2022

Purpose: To determine the relationship between adherence to anti-diabetic medication and depression among patients with diabetes mellitus in three hospitals in Chinese.Methods: This research utilized a quantitative and descriptive design, and included 200 diabetic patients who fulfilled the inclusion criteria for recruitment through a convenient sampling technique. The study applied Beck’s depression inventory II scale for assessment of depression, and a questionnaire for adherence to anti-diabetic medication.Results: A total of 64 (32 %) participants had diabetes for 6 to 10 years. There was a high level of adherence to anti-diabetic medication in 96 patients (48 %); 74 participants (37 %) had moderate adherence to anti-diabetic medication, while 30 patients (15 %) had low adherence. A majority of the patients (181, 90.5 %) had no depression. Six (6) patients (3 %) had mild mood disturbance, 2 patients (1 %) had borderline clinical depression, while 11patients (5.5 %) experienced moderate depression.Adherence to anti-diabetic medication was not associated with depression (p = 0.068). However, depression was associated with age ˃ 50 years (p = 0.041), female sex (p = 0.043), long duration of illness (> 5-years) (p = 0.048), and presence of one or more comorbidities (p = 0.049).Conclusion: There was no association between adherence to anti-diabetic medication and depression among diabetic patients.
Keywords: Adherence to anti-diabetic medication, Beck’s depression inventory II, Depression, Diabetes mellitus
Utilization of adjusted body weight for dosing unfractionated heparin in obese patients with venous thromboembolism: A retrospective matched cohort study
Authors: Alessa Mohammed , Gramish Jawaher , Almodaimegh Hind , Khobrani Moteb A. , Hornsby Lori , Alhifany Abdullah A. 
Published: January 2022

Purpose: To evaluate the effectiveness of adjusted body weight (AjBW)-based dosing of unfractionated heparin (UFH) in obese patients vis a vis actual body weight (ABW)-based dosing in non-obese patients with venous thromboembolism (VTE).Methods: A retrospective chart review was conducted for obese and non-obese patients initiated on UFH for treating VTE from September 2013 to December 2014. Patients were excluded from the study if they were under 18 years old, developed heparin-induced thrombocytopenia during treatment, received thrombolytic therapy prior to UFH, or received UFH at a dose that did not follow the institution’s protocol. The primary objective was to assess the efficacy of dosing UFH based on AjBW in achieving a therapeutic activated partial thromboplastin time (aPTT) within the first 24 h in obese patients, in comparison to the standard ABW-dosing for non-obese.Results: Of the 57 patients included in the study, 27 patients (47.4 %) were obese, and 30 patients (52.6 %) were non-obese; 16 (59.25 %) of the obese patients achieved a therapeutic aPTT within the first 24 h of AjBW-based dosed UFH, while 18 (60 %) of the non-obese patients achieved a therapeutic aPTT within the first 24 h of ABW-based dosed UFH (p = 0.45).Conclusion: AjBW-based dosing of UFH in obese patients demonstrates comparable efficacy to ABWbased dosing in non-obese patients.
Keywords: Obesity, Unfractionated heparin, Venous thromboembolism, Adjusted body weight, Ideal body weight
Effect of miR-200c on nasopharyngeal carcinoma and the probable molecular regulatory mechanism involved
Authors: Xu Sheng , He Wenxia 
Published: January 2022

Purpose: To determine the effect of micro-ribonucleic acid-200c (miR-200c) on biological function of nasopharyngeal carcinoma, and the likely molecular regulatory mechanism involved.Methods: Thirty (30) nasopharyngeal carcinoma tissues and para-cancerous normal tissues were taken from patients undergoing surgery in Maternal and Child Health Hospital of Hubei Province, Wuhan from September 2018 to January 2020. The expression levels of miR-200c in the two types of tissues were determined. Cells of human nasopharyngeal carcinoma cell line HNE1 were cultured to about 70 % growth prior to transfection with blank plasmid, PINI and miR-200c analogs. After 48 h of culture, control group, PINI group, and miR-200c over-expression + PINI group were obtained. The expression levels of PINI and changes in centrosomes in each group were measured, and changes in cell migration in each group were determined using Transwell migration assay.Results: Compared with para-cancerous normal tissues, the expression level of miR-200c in nasopharyngeal carcinoma was significantly increased (p < 0.01). Compared with the control group, the PINI expression level and cell migration ability in miR-200c overexpression tissue were markedly decreased, while the percentage of extra centrosomes was markedly increased. Compared to miR-200c over-expression tissue, the PINI expression level and cell migration ability in the miR-200c overexpression + PINI group were markedly raised, while the percentage of extra-central somatic cells was significantly decreased (p < 0.05).Conclusion: MiR-200c may inhibit the migration of nasopharyngeal carcinoma cells by inhibiting the expression of PINI and inhibiting abnormal expansion of centrosomes.
Keywords: MiR-200c, Nasopharyngeal carcinoma, Biological function, Molecular regulatory mechanism
Expression levels of apoptotic factors in a rat model of corticosteroid-induced femoral head necrosis
Authors: Fu Yongliang , Han Xiaoqiang , Xue Jiangang , Bai Hao , Sun Haibiao 
Published: January 2022

Purpose: To study the expression levels of apoptotic factors in corticosteroid-mediated femoral head necrosis (FHN) in rats.
Methods: Sprague-Dawley (SD) rats (n = 60) bred adaptively for one week were randomly assigned to control and model groups (30 rats/group). A rat model of corticosteroid-induced femoral head necrosis was established. Then, 3 mL of blood drawn from the inferior vena cava of each rat was used for the assay of the expression levels of osteoprotegerin (OPG) and osteoclast differentiation factor (RANKL) in each group using enzyme-linked immunosorbent assay (ELISA). The caspase-3- and Bcl-2-+ve cells in each group were determined with immunohistochemical method.
Results: Relative to control, serum OPG level of model group was significantly decreased, while the RANKL level was markedly raised (p < 0.05). The degree of empty lacunae in the model rats was markedly increased, relative to control. Caspase-3-+ve cells were more numerous in the model group than in control, while Bcl-2-positive cells were markedly decreased compared to control (p < 0.05).
Conclusion: Apoptosis occurs in the rat model of femoral head necrosis. Glucocorticoids may regulate the apoptotic process by upregulating caspase-3 and inhibiting Bcl-2. This provides a novel lead for FHN therapy.
Keywords: Femoral head necrosis, Corticosteroid, Glucocorticoid, Apoptosis
Characterization and biodistribution of trans resveratrol-PEG-folic acid-gold nanoparticle conjugates
Authors: Gumala Azhoma , Sutriyo  , Saputri Fadlina Chany 
Published: January 2022

Purpose: To evaluate the characteristics and biodistribution of trans resveratrol-PEG-folic acid-gold nanoparticle conjugates (rsv-PEG-FA-AuNP).
Methods: Gold nanoparticles were produced by citric reduction followed by conjugation of PEG-folic acid and resveratrol. Characterization of rsv-PEG-FA-AuNP conjugates including their particle size, zeta potential, and by Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM) was carried out. Biodistribution study of rsv-PEG-FA-AuNP was carried out using female Sprague Dawley rats. Biodistribution data were obtained from high performance liquid chromatography (HPLC) analysis.
Results: The mean particle size and zeta potential of rsv-PEG-FA-AuNP were 249.03 ± 10.31 and - 36.33 ± 3.12 mV, respectively. TEM images showed rsv-PEG-FA-AuNP conjugates formed spherical shape. Rsv-PEG-FA-AuNP conjugates found in plasma, kidney (1.90 ± 0.20 μg/g), spleen (2.65 ± 1.18 μg/g), liver (1.74 ± 0.03 μg/g), and lung (1.82 ± 0.12 μg/g), after 90 minutes intravenous administration (i.v.) in female Sprague Dawley rats. No free resveratrol was found in plasma, kidney, or spleen after i.v administration in female dawdle Sprague Dawley rats.
Conclusion: Resveratrol-PEG-FA-AuNP conjugates appear to be a potential chemotherapy delivery system for active targeting purposes because of its longer systemic circulation and its accumulation in the kidney.
Protective effect of Rhus chinensis Mill. extract against liver cirrhosis in rats
Authors: Pan Zirong , Cheng Qiang , Chen Heyan , Lin Longhai , Liao Weijia , Guo Donglai , Zhuang Jianmin 
Published: January 2022

Purpose: To study the effect of Rhus chinensis Mill. extract (RCME) on diethylnitrosamine (DEN)-induced liver cirrhosis in rats.
Methods: RCME was obtained by extracting the dried Rhus chinensis Mill. in water. Liver cirrhosis rat model was prepared by injecting with DEN once a week for 8 weeks. After 8th-week of RCME treatment, biochemical index and oxidative stress were determined in DEN-induced liver cirrhosis in rats.
Results: Compared with model group, plasma concentrations of alanine transaminase (ALT, 125.3 ± 4.1 U/L) and aspartate aminotransferase (AST, 152.4 ± 3.5 U/L) decreased significantly (p < 0.01) in the 8th week. Rhus chinensis Mill. extract (RCME) significantly decreased malondialdehyde (MDA, 0.18 ± 0.02 umol/L) and superoxide dismutase (SOD, 0.76 ± 0.05 U/mg protein) in DEN-induced liver cirrhosis in rats (p < 0.01) when compared with model group.
Conclusion: RCME protects against diethylnitrosamine-induced liver cirrhosis in rats. However, further investigations are required to ascertain the plant extract’s suitability for the clinical management of liver cirrhosis.
Effect of angiotensin converting enzyme gene polymorphism on patients with in-stent restenosis after percutaneous coronary intervention
Authors: Abdelaziz Tarek A. , Mohamed Randa H. , Balata Gehan F. , El-Azzazy Omar Y. 
Published: January 2022

Purpose: To evaluate the association between common single nucleotide polymorphisms (SNPs) in angiotensin converting enzyme (ACE) gene and the risk of in-stent restenosis (ISR) and/or the response to angiotensin converting enzyme inhibitor ACEI in individuals with stable coronary artery disease (CAD) after stent implantation.
Methods: The total population of this study consisted of 200 Egyptian individuals divided into 2 groups - in-stent restenosis (ISR) and non ISR group). Genomic DNA was withdrawn from EDTA whole blood applying a spin column approach and ACE gene insertion/deletion (I/D) polymorphisms were determined by polymerase chain reaction (PCR).
Results: Carriers of allele D of ACE gene were significantly more liable to ISR occurrence. However, carriers of allele I were significantly more liable to ISR occurrence after administration of ACEI. There is a negative interaction between DD genotype of ACE gene and ACEI administration on ISR after percutaneous coronary intervention (PCI). However, there is a positive interaction between II and ID genotype of ACE gene and ACEI administration on ISR after PCI with bare metal stents (BMS).
Conclusion: It is beneficial to implement ACEI in therapeutic regimen in individuals with ID or II genotypes of ACE gene, especially with BMS implementation.
Therapeutic effect of cycloserine combined with anti-tuberculosis drugs in the treatment of multidrug-resistant tuberculosis
Authors: Zhao Yang , Si Mabin , Li Zhihui , Yu Xiulei 
Published: January 2022

Purpose: The present study analyzes the comprehensive therapeutic effect of cycloserine, in combination with anti-tuberculosis drugs using chest X-ray and chest CT (computed tomography) scan techniques.
Methods: A total of 90 patients, diagnosed with multidrug resistant tuberculosis (MDR TB) were subjected to chest x-ray and CT scan before and after treatment in the two groups. Different views such as sagittal, coronal, lung window and multiplanar imaging of mediastinal window were taken. Some parameters such as case detection rate (CDR) in chest X-ray and CT scan and comprehensive curative effect were observed in two groups. Further, the changes in chest CT signs in addition to absorption of focus, cavity closure and changes in CT extra pulmonary signs were also observed.
Results: The clinical profile of the patients and the course of disease were statistically insignificant (p > 0.05). Total effectiveness rate and case detection rate (CDR) values exhibited a significant difference between the groups (p < 0.05). Lung consolidation, nodules and cavities significantly improved in both groups before and after the treatment (p < 0.05). Both groups showed significant improvements in extrapulmonary signs in CT scan (p < 0.05) after the treatment.
Conclusion: Based on the study outcomes, the CT scan method has good potentials for diagnosing and treating MDR TB at the early stages. Further, it can clarify the signs and outcomes of the disease at early stages, thus providing the medical fraternity a great opportunity to cure the disease.
MiR-557 downregulation lowered cell proliferation and malignancy in glioblastoma by targeting ADAM17
Authors: Li Zongping , Liu Hongyuan , Tang Zhi , Zhong Qi , Yang Xu , Zhang Ye , He Pengcheng , Wang Xiaoyi 
Published: January 2022

Purpose: To examine the role of miR-557 in the pathogenesis and development of glioblastoma (GBM) and its potential regulatory mechanism.
Methods: Dysregulation of miR-557 in GBM cells was determined using quantitative (real-time) PCR (qPCR). Cell proliferative ability, migratory activity and invasive ability were measured using the MTT colorimetric assay, wound healing assay and Transwell chambers assay. The direct target mRNA of miR-557 was predicted, and further validated using TargetScan and luciferase assay, respectively.
Results: Compared with human astrocytes (HAs), the downregulation of miR-557 was observed in human GBM cell lines. In U-251MG and A172 cells that overexpressed miR-557 mimics, the level of proliferation was significantly reduced, the wound width was larger, and the number of invaded cells wan decreased than that of NC mimics. Predictive results from TargetScan and results of luciferase assay demonstrated that miR-557 directly targets the 3’-untranslated region (3’-UTR) of ADAM17. In A172 cells and U-251MG cells, the upregulation E-cadherin (E-cad) and the downregulation of N- cadherin (N-cad) and vimentin (Vim) were caused by miR-557 mimics as compared with NC mimics. Expression of ADAM17, NICD, HES1, and EGFR was downregulated by miR-557 mimics and upregulated by miR-557 inhibitors in A172 cells and U-251MG cells.
Conclusion: Downregulation of miR-557 accelerates GBM cell growth and malignancy by directly targeting ADAM17 3’-UTR, providing a prognostic and potential therapeutic factor for new drug discovery in GBM.
Curcuma longa Linn extract suppresses neuronal apoptosis induction by sevoflurane via activation of the ERK1/2 pathway
Authors: Yu Zhou , Yan Yao , Lou Ying 
Published: January 2022

Purpose: To investigate Curcuma longa Linn against neuronal damage induced by exposure to sevoflurane during surgical procedures.
Methods: A sealed box made of transparent glass was used for anaesthetic exposure of neurons. The neurons were exposed to Curcuma longa Linn at doses of 1.5, 3, 6 and 12 μM prior to viability assessment using MTT assay. The effect of Curcuma longa Linn treatment on protein expression was determined using western blotting.
Results: Sevoflurane exposure led to significant and time-dependent reductions in neuronal proliferation, when compared to unexposed cells (p < 0.05). Curcuma longa Linn at doses of 1.5, 3, 6 and 12 μM significantly decreased sevoflurane-mediated neuronal apoptosis. It reduced cleaved caspase-3 and Bax levels in neurons. However, the Curcuma longa Linn-mediated inhibition of sevoflurane-induced neuronal apoptosis was significantly suppressed by VPC23019 (p < 0.05). The p- ERK1/2 level was dose-dependently up-regulated in neurons exposed to sevoflurane on treatment with Curcuma longa Linn. Moreover, VPC23019 reversed the upregulatory effect of Curcuma longa Linn on p-ERK1/2 expression in sevoflurane-exposed neurons (p < 0.05).
Conclusion: Curcuma longa Linn reversed sevoflurane-induced neuronal apoptosis by elevating p- ERK1/2 expression. Therefore, Curcuma longa Linn exerts inhibitory effect on anaesthesia-induced apoptosis in neurons, and may be useful for the treatment of this condition.
PŘESTUPKY V SILNIČNÍ DOPRAVĚ V ČESKÉ REPUBLICE, BODOVÝ SYSTÉM ŘIDIČŮ V ČESKÉ REPUBLICE, NĚMECKU A POLSKU A ŘEŠENÍ DOPRAVNÍHO PŘESTUPKU POLICIÍ ČR A NA OBECNÍM ÚŘADU OBCE S ROZŠÍŘENOU PŮSOBNOSTÍ S NÁVRHY ZMĚN
Authors: Kleprlík Jaroslav , Světlá Klára 
Published: January 2022

Příspěvek představuje základní právní předpisy EU a právní předpisy ČR upravující oblast dopravních nehod a přestupků v silniční dopravě. Uvádí hlavní dopravní přestupek, kterým je překročení nejvyšší povolené rychlosti. Stručně analyzuje statistiky dopravních nehod v ČR ve vazbě na statistiku přestupků v silniční dopravě celostátní i ve vybrané obci s rozšířenou působností. Komentuje a porovnává na vybraných přestupcích bodový systém v ČR, v Německu a v Polsku. Popisuje postup Policie ČR a technologický postup na obecním úřadu obce s rozšířenou působností při zpracování přestupku řidiče. V příspěvku jsou navrženy konkrétní změny v postupu Policie ČR a především změny v postupu úředníka na obecním úřadě obce s rozšířenou působností. Tyto změny mají vést k rychlejšímu zpracování přestupku, racionalizaci práce úředníka a také k vyšší informovanosti řidiče, který přestupky spáchal.
Klíčová slova
IMPLEMENTÁCIA CESTNÉHO BALÍČKA EÚ 2020 A ODHAD JEHO DOPADOV VO VYBRANOM ČLENSKOM ŠTÁTE: SLOVENSKO
Authors: Cződörová Renáta , Gnap Jozef 
Published: January 2022

Európska únia prijala v oblasti cestnej dopravy „cestný balíček“ viacerých opatrení, ktorých cieľom je pomôcť odvetviu zachovať si konkurencieschopnosť v rámci sociálne spravodlivej transformácie smerom k čistej energii a digitalizácii. Cieľom predloženého balíčka do ďalšieho legislatívneho procesu na úrovni EÚ je podľa Európskej komisie riešiť otázky veľkej preťaženosti ciest a znečistenia, ale tiež reagovať na rastúci počet subjektov v cestnej nákladnej doprave a ich pracovné a sociálne podmienky. Cieľom tohto príspevku je skúmanie vplyvu týchto nových legislatívnych zmien na podnikateľské prostredie v cestnej doprave vo vybranom členskom štáte EÚ v Slovenskej republike. Boli odhadnuté vybrané náklady na implementáciu balíčka na prevádzkovateľov cestnej dopravy.
MOŽNOSTI STAVEBNÉHO PREPOJENIA BRATISLAVSKÉHO LETISKA K SIETI ŽSR
Authors: Bulková Zdenka , Zitrický Vladislav , Gašparík Jozef 
Published: January 2022

Koľajová doprava v okolí Bratislavského letiska funguje len pre účely nákladnej prepravy primárne v podobe dovozu pohonných látok, čomu je uspôsobená aj daná infraštruktúra. Potenciál Bratislavského letiska M. R. Štefánika v súčasnosti nie je vyčerpaný, avšak možnosti prístupu k tomuto letisku sú značne vymedzené stupňom rozvinutia jednotlivých druhov infraštruktúry. Železničná infraštruktúra, ktorá v súčasnosti slúži potrebám letiska M. R. Štefánika existuje v podobe železničnej vlečky zaústenej do železničnej siete odbočkou Letisko na neelektrifikovanú jednokoľajnú trať smerujúcu do železničnej stanice Podunajské Biskupice. S využitím pre účely osobnej dopravy sa v súčasnej podobe priveľmi nepočíta najmä pre vzdialenosť od terminálu letiska, pričom výhodou tiež nie je ani zaústenie do železničnej siete. Článok sa zaoberá možnosťami napojenia bratislavského letiska k sieti ŽSR. Základom návrhu napojenia bratislavského letiska vhodnou infraštruktúrou k sieti ŽSR v rámci železničnej osobnej dopravy je stanovenie vhodného prestupného miesta. Jednou z logických priorít je umiestnenie prestupného miesta čo najbližšie k terminálu leteckej dopravy bratislavského letiska v rámci priestorových možností a technických podmienok.
SYSTEMATICKÁ REŠERŠE K OPTIMALIZACI UMÍSTĚNÍ LOGISTICKÝCH OBJEKTŮ AKCENTUJÍCÍ ROZVOJ E-COMMERCE A FAKTOR COVID-19
Authors: Roubíček Filip , Kolář Petr 
Published: January 2022

Hlavním cílem článku je důkladně shrnout nejnovější publikované výsledky výzkumu, zaměřeného na optimální umístění logistických center elektronického obchodování a umístění logistických center, z pohledu nabídky a poptávky na trhu logistických nemovitostí, v němž rozvoj elektronického obchodu hraje klíčovou roli. Tento článek se zaměřuje na práci se sekundárními daty, při zejména formě systematické rešerše aktuálních, relevantních a kvalitních zdrojů k jeho tématu, vysvětlujících dynamiku rozvoje e-commerce, s ohledem na problematiku rozhodovacích mechanismů, při optimalizaci umisťování logistických objektů, jako jsou distribuční centra, nebo sklady. Lze identifikovat rozvoj kvalitativního, kvantitativního i hybridního výzkumu, ve kterém se publikovaní autoři zabývají při aplikaci různých metodologických východisek a dat korelací mezi rozvojem e-commerce a strategiemi umísťování distribučních center. Nezřídka ale užívají metody, nebo modely s omezeným potenciálem replikovatelnosti mimo geograficky vymezený trh, s mnoha nejednoznačnými předpoklady, které jsou diskutabilně definovány.