bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dendrimer-modified gelatin methacrylate hydrogels carrying adipose-derived stromal/stem cells promote cartilage regeneration
Authors: Liu Fengyi , Wang Xu , Li Yuzhou , Ren Mingxing , He Ping , Wang Lu , Xu Jie , Yang Sheng , Ji Ping 
Published: January 2022

AbstractBackgroundCartilage defects pose a significant burden on medical treatment, leading to an urgent need to develop regenerative medicine approaches for cartilage repair, such as stem cell therapy. However, the direct injection of stem cells can result in insufficient delivery or inaccurate differentiation. Hence, it is necessary to choose appropriate stem cell delivery scaffolds with high biocompatibility, injectability and chondral differentiation induction ability for cartilage regeneration.MethodsIn this study, the photocrosslinked gelatin methacrylate (GelMA) hydrogel with high cell affinity and plasticity was selected and strengthened by incorporating methacrylic anhydride-modified poly(amidoamine) (PAMAM-MA) to fabricate an adipose-derived stromal/stem cells (ASCs) delivery scaffold for cartilage repair. The physiochemical properties of the GelMA/PAMAM-MA hydrogel, including the internal structure, stability and mechanical properties, were tested. Then, ASCs were encapsulated into the hydrogels to determine the in vitro and in vivo chondrogenic differentiation induction abilities of the GelMA/PAMAM-MA hydrogel.ResultsCompared with the GelMA hydrogel, the GelMA/PAMAM-MA hydrogel exhibited more uniform structure, stability and mechanical properties. Moreover, on the basis of good biocompatibility, the hybrid hydrogel was proven to exert a sufficient ability to promote cartilage regeneration by in vitro three-dimensional (3D) culture of rASCs and in vivo articular cartilage defect repair.ConclusionsThe injectable photocrosslinked GelMA/PAMAM-MA hydrogel was proven to be a capable stem cell carrier for cartilage repair and provides new insight into the design strategy of stem cell delivery scaffolds.
Disruption of hematopoiesis attenuates the osteogenic differentiation capacity of bone marrow stromal cells
Authors: Wang Changzhen , Ning Hongmei , Gao Jiao , Xue Teng , Zhao Ming , Jiang Xiaoxia , Zhu Xiaoming , Guo Ximin , Li Hong , Wang Xiaoyan 
Published: January 2022

Abstract
Background
The homeostasis of mesenchymal stem cells (MSCs) is modulated by both their own intracellular molecules and extracellular milieu signals. Hematopoiesis in the bone marrow is maintained by niche cells, including MSCs, and it is indispensable for life. The role of MSCs in maintaining hematopoietic homeostasis has been fully elucidated. However, little is known about the mechanism by which hematopoietic cells reciprocally regulate niche cells. The present study aimed to explore the close relationship between MSCs and hematopoietic cells, which may be exploited for the development of new therapeutic strategies for related diseases.

Methods
In this study, we isolated cells from the offspring of Tie2Cre + and Ptenflox/flox mice. After cell isolation and culture, we investigated the effect of hematopoietic cells on MSCs using various methods, including flow cytometry, adipogenic and osteogenic differentiation analyses, quantitative PCR, western bloting, and microCT analysis.

Results
Our results showed that when the phosphatase and tensin homolog deleted on chromosome 10 (Pten) gene was half-deleted in hematopoietic cells, hematopoiesis and osteogenesis were normal in young mice; the frequency of erythroid progenitor cells in the bone marrow gradually decreased and osteogenesis in the femoral epiphysis weakened as the mice grew. The heterozygous loss of Pten in hematopoietic cells leads to the attenuation of osteogenic differentiation and enhanced adipogenic differentiation of MSCs in vitro. Co-culture with normal hematopoietic cells rescued the abnormal differentiation of MSCs, and in contrast, MSCs co-cultured with heterozygous null Pten hematopoietic cells showed abnormal differentiation activity. Co-culture with erythroid progenitor cells also revealed them to play an important role in MSC differentiation.

Conclusion
Our data suggest that hematopoietic cells function as niche cells of MSCs to balance the differentiation activity of MSCs and may ultimately affect bone development.
Cell maturation influences the ability of hESC-RPE to tolerate cellular stress
Authors: Viheriälä Taina , Hongisto Heidi , Sorvari Juhana , Skottman Heli , Nymark Soile , Ilmarinen Tanja 
Published: January 2022

Abstract
Background
Transplantation of human pluripotent stem cell-derived retinal pigment epithelium (RPE) is an urgently needed treatment for the cure of degenerative diseases of the retina. The transplanted cells must tolerate cellular stress caused by various sources such as retinal inflammation and regain their functions rapidly after the transplantation. We have previously shown the maturation level of the cultured human embryonic stem cell-derived RPE (hESC-RPE) cells to influence for example their calcium (Ca2+) signaling properties. Yet, no comparison of the ability of hESC-RPE at different maturity levels to tolerate cellular stress has been reported.

Methods
Here, we analyzed the ability of the hESC-RPE populations with early (3 weeks) and late (12 weeks) maturation status to tolerate cellular stress caused by chemical cell stressors protease inhibitor (MG132) or hydrogen peroxide (H2O2). After the treatments, the functionality of the RPE cells was studied by transepithelial resistance, immunostainings of key RPE proteins, phagocytosis, mitochondrial membrane potential, Ca2+ signaling, and cytokine secretion.

Results
The hESC-RPE population with late maturation status consistently showed improved tolerance to cellular stress in comparison to the population with early maturity. After the treatments, the early maturation status of hESC-RPE monolayer showed impaired barrier properties. The hESC-RPE with early maturity status also exhibited reduced phagocytic and Ca2+ signaling properties, especially after MG132 treatment.

Conclusions
Our results suggest that due to better tolerance to cellular stress, the late maturation status of hESC-RPE population is superior compared to monolayers with early maturation status in the transplantation therapy settings.
Fractional versions of Minkowski-type integral inequalities via unified Mittag-Leffler function
Authors: Zhou Shuang-Shuang , Farid Ghulam , Ahmad Ayyaz 
Published: January 2022

AbstractWe present unified versions of Minkowski-type fractional integral inequalities with the help of fractional integral operator based on a unified Mittag-Leffler function. These inequalities provide new as well as already known fractional versions of Minkowski-type inequalities.
Inequalities for fractional Riemann–Liouville integrals of certain class of convex functions
Authors: Farid Ghulam , Pec̆arić Josip , Nonlaopon Kamsing 
Published: January 2022

AbstractFractional calculus operators play a very important role in generalizing concepts of calculus used in diverse fields of science. In this paper, we use Riemann–Liouville fractional integrals to establish generalized identities, which are further applied to obtain midpoint and trapezoidal inequalities for convex function with respect to a strictly monotone function. These inequalities reproduce midpoint and trapezoidal inequalities for convex, harmonic convex,p-convex, and geometrically convex functions. Also, some new inequalities can be generated via specific strictly monotone functions.
Unsteady non-Newtonian fluid flow with heat transfer and Tresca’s friction boundary conditions
Authors: Paoli Laetitia 
Published: January 2022

AbstractWe consider an unsteady non-isothermal flow problem for a general class of non-Newtonian fluids. More precisely the stress tensor follows a power law of parameterp, namely$\sigma = 2 \mu ( \theta , \upsilon , \| D(\upsilon ) \|) \|D( \upsilon ) \|^{p-2} D(\upsilon ) - \pi \mathrm{Id}$σ=2μ(θ,υ,∥D(υ)∥)∥D(υ)∥p−2D(υ)−πIdwhereθis the temperature,πis the pressure,υis the velocity, and$D(\upsilon )$D(υ)is the strain rate tensor of the fluid. The problem is then described by a non-stationaryp-Laplacian Stokes system coupled to an$L^{1}$L1-parabolic equation describing thermal effects in the fluid. We also assume that the velocity field satisfies non-standard threshold slip-adhesion boundary conditions reminiscent of Tresca’s friction law for solids. First, we consider an approximate problem$(P_{\delta })$(Pδ), where the$L^{1}$L1coupling term in the heat equation is replaced by a bounded one depending on a small parameter$0 < \delta \ll 1$0<δ≪1, and we establish the existence of a solution to$(P_{\delta })$(Pδ)by using a fixed point technique. Then we prove the convergence of the approximate solutions to a solution to our original fluid flow/heat transfer problem asδtends to zero.
Older people in custody in a forensic psychiatric facility, prevalence of dementia, and community reintegration needs: an exploratory analysis
Authors: Stoliker Bryce E. , Kerodal Ashmini G. , Jewell Lisa M. , Brown Kelsey , Kent-Wilkinson Arlene , Peacock Shelley , O’Connell Megan E. , Wormith J. Stephen 
Published: January 2022

Abstract
Background
Across much of the developed world, the number of older people in custody has been increasing, which presents challenges for correctional systems due to the complex social, medical and mental health needs of this subgroup, especially those living with dementia. The present study therefore aimed to increase insight into the extent to which older people in custody are (a) potentially living with dementia and (b) receiving appropriate supports/services (particularly, with respect to community reintegration).

Results
Cross-sectional data were drawn from a sample of 29 older people in custody and 20 correctional health care professionals at a regional forensic psychiatric hospital in a medium-sized Canadian city. In general, analyses revealed that: (a) scores from a modified version of the Community Screening Instrument for Dementia (CSI‘D’) suggest that 45% of older individuals screened positive for dementia; (b) 35% of Social Workers and 25% of Primary Nurses (i.e., RNs/RPNs) suspected that at least one older individual on their caseload has dementia, and there was adequate agreement between health staffs’ perception of the presence or absence of dementia and the CSI‘D’ assessment; (c) varying supports/services may be required for older individuals’ successful community reintegration and living; and (d) Social Workers and Primary Nurses generally lack training/education to adequately support older people in custody.

Conclusions
A substantial number of older people in custody may experience age-related challenges, including dementia. This necessitates the development and implementation of programming to effectively address older individuals’ needs during incarceration and community reintegration and living.
Effects of a school-based intervention on levels of anxiety and depression: a cluster-randomized controlled trial of the MindPower program in ten high schools in Norway
Authors: Sælid Gry Anette , Czajkowski Nikolai Olavi , Aarø Leif Edvard , Andersen John Roger , Idsøe Thormod , Helleseter Miguel Delgado , Holte Arne 
Published: January 2022

Abstract
Background
The previous decades have shown increased symptoms of depression and anxiety among adolescents. To promote mental health and reduce mental illness, the government of Norway has, as in other countries, pledged that all schools must incorporate life-skills education. We report results from an evaluation of MindPower, a modification of the Coping With Depression (CWD) course, delivered universally in the classroom to secondary high school students, aged 15–16 years, in one county in Norway. The aim of the study was to evaluate the effect of MindPower on symptoms of depression and anxiety.

Methods
We utilized a two-groups` delayed intervention design where 110 first year high school classes were randomized into one of two intervention groups (IG1 and IG2). IG1 participated in MindPower while IG2 served as a control group for four months until the intervention started also in this group. IG1 and IG2 responded to questionnaires before and after the eight weeks course, at the start of the first and the second booster session, and at the five months follow up. Questionnaires, including online versions of the Hopkins Symptom Checklist (SCL-8) and the Reynolds Adolescent Depression Scale (RADS-2:SF), were administered to 1673 out of a total of 2384 students. SCL-levels were also compared with those from a large population study (UngData).

Results
According to mixed model analyses, SCL-8 and RADS-2:SF showed significant baseline differences between IG1 and IG2. In IG1 and IG2, both SCL-8 and RADS-2:SF showed a small but significant increase in mean scores throughout the study period, with markedly lower mean scores among boys. The SCL-levels were first lower for both girls and boys and then after the completion of MindPower the SCL-levels, equal to the SCL-levels in UngData.

Conclusions
No effects of the intervention were found. This large universal school-based trial suffered from considerable drop-out of participants. Experiences from implementation and evaluation of universal mental health promotion and preventive school interventions are thoroughly discussed, including, preparation, resources, support, time, realistic expectations, teacher selection and training, implementation, research designs and more. Several empirically based, practical advices are presented.
Clinical Trial registration
27/08/2018. Registration number NCT03647826.
The influence of malocclusion on social aspects in adults: study via eye tracking technology and questionnaire
Authors: Gasparello Gil Guilherme , Júnior Sergio Luiz Mota , Hartmann Giovani Ceron , Meira Thiago Martins , Camargo Elisa Souza , Pithon Matheus Melo , Tanaka Orlando 
Published: January 2022

Abstract
Background
Smile esthetics has a strong influence on perception, attractiveness, and personal characteristics. It is unknown how malocclusions may influence the appearance of the individual's smile. This study aimed to investigate whether malocclusion affects the visual perception of esthetics, age, employability, honesty, intelligence and to meet its obligation in time in middle-aged adults. Facial frontal smiling photographs of a male and a female middle-aged adult with 3 different malocclusions were shown to and evaluated by 90 laypeople (non-dentists), divided into groups: young adults (14–44 years), middle-aged adults (45–59 years), and elders (over 60 years). The index of treatment need (IOTN) was used, and IOTN 1, 5, and 8 images were created in Photoshop using the male and female photographs. In total, 6 photographs were evaluated, 3 areas of interest (AOI)—eyes, nose, and mouth—were created for statistical comparison. The Ogama and Eye Tribe tracker were used in conjunction to measure eye tracking. A visual analog scale (VAS) was employed with a questionnaire surveying individuals’ perception of age, employability, honesty, intelligence, and ability to meet obligations. Kruskal–Wallis, one-way analysis of variance, Pearson’s—chi-squared, and Pearson correlation test were applied.

Results
No statistical difference was found in complete fixation time and time until the first fixation for each AOI for eye-tracking. VAS showed statistical differences in the male and female IOTN 1 images when compared with the IOTN 5 and 8 for both models, and there was no difference between genders in the IOTN 1. As the perceived age of the model increased, the chances of getting hired decreased. Employability, honesty, intelligence, and ability to meet obligations showed higher values for IOTN 1. There were significant differences between age ranges; perception of intelligence in the female IOTN 1 model (p = 0.002) and IOTN 8 model (p < 0.001) and honesty between young adults and middle-aged adults in the male IOTN 1 and 8 images (p < 0.001).

Conclusions
There was a difference between age groups in the perception of honesty and intelligence. A well-balanced and attractive was perceived as more youthful and attractive in both genders and may increase the chance of being selected for a job interview or being hired.
Responses of leaf hydraulic traits of Schoenoplectus tabernaemontani to increasing temperature and CO2 concentrations
Authors: Zhao Yao , Sun Mei , Guo Huijun , Feng Chunhui , Liu Zhenya , Xu Junping 
Published: January 2022

Abstract
Background
Against the background of a changing climate, the responses of functional traits of plateau wetland plants to increasing temperatures and CO2 concentrations need to be understood. Hydraulic traits are the key for plants to maintain their ecological functions and affect their growth and survival. However, few studies have comprehensively considered the response strategies of wetland plants' hydraulic traits to environmental changes in the context of water and matter transport, loss, and retention. According to the latest IPCC prediction results, we performed experiments under increased temperature (2 °C) and CO2 levels (850 μmol/mol) in an artificial Sealed-top Chamber (STC) to investigate the responses of the hydraulic characteristics of Schoenoplectus tabernaemontani, the dominant species in plateau wetlands in China.

Results
Compared with the CK group, net photosynthetic rate, transpiration rate, stomatal length, cuticle thickness, vascular bundle length, vascular bundle width, and vascular bundle area of S. tabernaemontani in the ET group were significantly reduced, whereas stomatal density and vein density increased significantly. Compared with the CK group, the hydraulic traits of S. tabernaemontani in the EC group were reduced considerably in stomatal length and cuticle thickness but increased dramatically in stomatal density, and there were no significant differences between other parameter values and the control group. Net photosynthetic rate was significantly positively correlated with stomatal length, cuticle thickness, and vascular bundle length, and stomatal conductance was significantly positively correlated with cuticle thickness. The transpiration rate was significantly positively correlated with cuticle thickness, epidermal cell area, vascular bundle length, vascular bundle width, and vascular bundle area. Regarding the hydraulic traits, there was a significant negative correlation between stomatal density and stomatal length, or cuticle thickness, and a significant positive correlation between the latter two. The epidermal cell area was significantly positively correlated with epidermal thickness, vascular bundle length, vascular bundle width, and vascular bundle area.

Conclusions
Increased temperature and CO2 levels are not conducive to the photosynthetic activity of S. tabernaemontani. Photosynthetic rate, stomatal density and size, vein density, epidermal structure size, and vascular bundle size play an essential role in the adaptation of this species to changes in temperature and CO2 concentration. In the process of adaptation, hydraulic traits are not isolated from each other, and there is a functional association among traits. This study provide a scientific basis for the management and protection of plateau wetlands.
A culturally adapted, social support-based, diabetes group visit model for Bangladeshi adults in the USA: a feasibility study
Authors: Shah Megha K. , Naing Sukyi , Kurra Nithin , Weber Mary Beth , Islam Nadia , Ali Mohammed K. , Narayan K. M. Venkat 
Published: January 2022

Abstract
Background
Interventions focused on weight loss can prevent, delay, and improve management of type 2 diabetes (T2D). However, implementation of these programs is challenging in diverse populations. South Asians have higher risk for T2D, yet to date, there have been limited programs for this community in the USA. The aim of this project was to develop and test the feasibility of a tailored group visit model for Bangladeshis with type 2 diabetes (T2D) or prediabetes based in primary care.

Design
Mixed-methods single-arm feasibility study.

Setting
An academic health center-based primary care clinic in Atlanta, Georgia.

Participants
Bangladeshi adults > 18 years old with T2D or prediabetes

Methods
In conjunction with a community-academic board, we conducted focus groups to tailor an existing evidence-based curriculum to a culturally acceptable intervention. Fourteen participants enrolled in the 16-week program focusing on healthy diet, exercise, and weight loss. The primary feasibility outcomes were number of sessions attended and participant satisfaction with the intervention. Weight, blood pressure, cholesterol, and HbA1C were measured at beginning and end of study. Participants were asked to evaluate each session on level of satisfaction. One tailed paired t tests were used to test significance of pre-post changes in outcomes.

Results
Key themes from the formative focus groups (n = 50) were closely tied to sociocultural beliefs and included: dietary patterns, physical activity perceptions, and healthcare access concerns. In the intervention, 10 of 14 participants had baseline and follow-up data. Participant attendance averaged 50%. Statistically significant reductions in mean weight (− 2%, 95%CI: − 3.1, 0.2 kg), systolic/diastolic blood pressure (− 12.7 mmHg [95%CI: − 23.2, − 2.2]/− 3.7 mmHg [95%CI: − 7.6, − 0.1], respectively), and triglycerides (− 62.6 mg/dl, 95%CI: − 123.1, − 2.0) were noted. Overall, participants reported high levels of satisfaction with the program.

Conclusion
A lifestyle program based in primary care is feasible and acceptable for Bangladeshi immigrants. Larger studies testing the effectiveness of group programs, in primary care, to improve cardiometabolic factors are important.

Trials registration
ClinicalTrials.gov; NCT03861546. Registered 28 February 2019.
Protocol of a pilot-scale, single-arm, observational study to assess the utility and acceptability of a wearable hydration monitor in haemodialysis patients
Authors: Sandys Vicki , Edwards Colin , McAleese Paul , O’Hare Emer , O’Seaghdha Conall 
Published: January 2022

Abstract
Background
Fluid overload has a high prevalence in haemodialysis patients and is an important risk factor for excess mortality and hospitalisations. Despite the risks associated with chronic fluid overload, it is clinically difficult to assess and maintain fluid status adequately. Current methods of fluid status assessment are either imprecise or time intensive. In particular, to date, no method exists to accurately assess fluid status during the interdialytic interval.

Objectives
This pilot study aimed to evaluate whether a prototype wearable hydration monitor can accurately and reproducibly detect fluid overload in the haemodialysis population when compared to haemodialysis and bioimpedance data.

Methods
A prospective, open-label, single-arm observational trial of 20 patients commenced in January 2021 in a single haemodialysis centre in Ireland, with a wearable hydration monitor, the Sixty device. The Sixty device uses diffuse reflectance spectroscopy to measure fluid levels at the level of the subdermis and uses machine learning to develop an algorithm that can determine fluid status. The Sixty device was worn at every dialysis session and nocturnally over a three-week observational period. Haemodialysis parameters including interdialytic weight gain, ultrafiltration volume, blood pressure, and relative blood volume were collected from each session, and bioimpedance measurements using the Fresenius body composition monitor were performed on 4 occasions as a comparator. The primary objective of this trial was to determine the accuracy and reproducibility of the Sixty device compared to bioimpedance measurements.

Conclusion
If the accuracy of the wearable hydration monitor is validated, further studies will be conducted to integrate the device output into a multi-parameter machine learning algorithm that can provide patients with actionable insights to manage fluid overload in the interdialytic period.

Trial registration
www.clinicaltrials.govNCT04623281. Registered November 10th, 2020.