bluuebunny/crossref_metadata_2025_split huggingface dataset:
A systematic review and meta-analysis comparing complications following total hip arthroplasty for systemic lupus erythematosus versus for non-systemic lupus erythematosus
Authors: Zeng Yirong , Huang Yiwei , Guan Danni , Li Yijin , Li Jiahao 
Published: January 2022

Abstract
Background: Osteonecrosis of the femoral head is one of the most severe complications in systemic lupus erythematosus(SLE) patients. Total hip arthroplasty(THA) is an effective treatment for femoral head necrosis. However, there is no consensus on the specific effect of THA on SLE patients. The objective of the present study was to review the current evidence regarding rates of THA complications and postoperative function in systemic lupus erythematosus.Methods: Two independent reviewers searched PubMed, Cochrane Library, and EMBASE from January 1, 2000, to December 29, 2021. The primary outcomes were postoperative complications, including deep vein thrombosis (DVT), hematoma, wound infection, dislocation, periprosthetic fracture, revision, mortality.Results: A total of 179 articles yielded 28 studies eligible for inclusion with 10 studies used for meta-analysis. This study found a statistically significant difference in DVT, dislocation, wound infection, periprosthetic fracture, and revision.Conclusions: This meta-analysis shows that SLE patients with THA are at an increased risk of DVT, wound infection, dislocation, periprosthetic fracture, revision, periprosthetic joint infection, following THA in comparison to non-SLE patients with THA. There was no adequate evidence to support the notion that the risk of seroma or hematoma following THA is increased in SLE. Also, there was no significant difference in HHS scores between SLE patients and non-SLE patients after THA.
Calcitonin Gene-related Peptide Attenuated Discogenic Low Back Pain by Inhibiting Microglia Activation Through the NLRP3/caspase-1 Signaling Pathway
Authors: Xie Weixin , Li Fan , Han Yi , Su Diansan , Yu Weifeng , Li Zhanchun , Xiao Jie 
Published: January 2022

Abstract
Discogenic low back pain (DLBP) is a multifactorial disease, associated with intervertebral disc degeneration. Calcitonin gene-related protein (CGRP) plays a critical role in pain processing, while the role in DLBP remains unclear. This study aims to investigate the anti-nociceptive role and related mechanisms of CGRP in DLBP. Here we established the DLBP rat and validated the model using histology and radiography. CGRP and minocycline were intrathecally injected respectively and the behavioral test was performed to determine hyperalgesia. Further, BV2 microglial cells and microglial activation agent lipopolysaccharide (LPS) were employed for the in vitro experiment. We observed obvious lumbar intervertebral disc degeneration and hyperalgesia at 12 weeks postoperation in DLBP group, with significantly activated microglia in the spinal cord. CGRP treatment significantly inhibited the upregulation of proinflammatory cytokines and NLRP3/caspase-1 expression induced by LPS in BV2 cells, whereas treatment with CGRP alone had little effect on BV2 cells. The intrathecal injection of CGRP into DLBP rats relieved mechanical and thermal hyperalgesia, reverted the microglial activation and decreased the expression of NLRP3/caspase-1, similar to the effects produced by minocycline. Our results provide evidence that microglial activation in the spinal cord play a key role in hyperalgesia in DLBP rats. CGRP effectively alleviates hyperalgesia and inhibits microglial activation in the spinal cord. Regulation of CGRP and microglial activation may provide a new strategy for ameliorating for DLBP.
Chronic Delta-9-Tetrahydrocannabinol (THC) Treatment Counteracts SIV-induced Modulation of Proinflammatory microRNA Cargo in Basal-ganglia-derived Extracellular- Vesicles
Authors: Kaddour Hussein , McDew-White Marina , Madeira Miguel M. , Tranquille Malik A. , Tsirka Stella E. , Mohan Mahesh , Okeoma Chioma M. 
Published: January 2022

Abstract
Background:Early invasion of the central nervous system (CNS) by human immunodeficiency virus (HIV) (1, 2), results in neuroinflammation, potentially through extracellular vesicles (EVs) and their micro RNAs (miRNA) cargoes (3, 4). Although the basal ganglia (BG) is a major target and reservoir of HIV in the CNS (5, 6), whether BG produces EVs and the effect of HIV and/or the phytocannabinoid― delta-9-tetrahydrocannabinol (THC) on BG-EVs and HIV neuropathogenesis remain unknown. Methods:We used simian immunodeficiency virus (SIV) model of HIV and THC treatment in rhesus macaques (7) to demonstrate for the first time that BG contains EVs (BG-EVs), and that BG-EVs cargo and function are modulated by SIV and THC. We also used primary astrocytes from the brains of wild type (WT) and CX3CR1+/GFP mice to investigate the significance of BG-EVs in CNS cells.Results:Significant changes in BG-EV associated miRNA specific to SIV infection and THC treatment were observed. BG-EVs from SIV infected rhesus macaques (SIV EVs) contain 11 significantly downregulated miRNA. Remarkably, intervention with THC led to significant upregulation of 37 miRNAs in BG-EVs (SIV-THC EVs). Most of these miRNAs regulate pathways related to inflammation/immune regulation, TLR signaling, Neurotrophin TRK receptor signaling, and cell death/response. BG-EVs activated WT and CX3CR1+/GFP astrocytes and reprogrammed astrocyte transcriptome in an EV and CX3CR1 dependent manners. Conclusions:Our findings reveal a role for BG-EVs as a vehicle with potential to disseminate HIV and THC induced changes within the CNS.
Characterization of New Multifunctional GH20 β-N-Acetylglucosaminidase From Chitinibacter sp. GC72 and Its Application in Converting Chitin Into N-Acetyl Glucosamine
Authors: Chen Yan , Zhou Ning , Chen Xueman , Wei Guoguang , Zhang Alei , Chen Kequan , Ouyang Pingkai 
Published: January 2022

Abstract
Background

N-acetyl glucosamine (GlcNAc), possesses many specific bioactivities and has been widely used in biomedical, food, and chemical industries. Alternatively, enzymatic hydrolysis of chitin into GlcNAc using chitinolytic enzymes was shown to be a more attractive approach in recent years, because of the green process and the excellent bioactivity of product. Therefore, it is of economic and environmental value to realize the efficient production of GlcNAc from abundant chitin resources.
Results

In this study, a gene encoding β-N-acetylglucosaminidase, designated NAGaseA, was cloned from Chitinibacter sp. GC72 and subsequently functional expressed in Escherichia coli BL21 (DE3). NAGaseA contains a glycoside hydrolase family 20 catalytic domain that shows low identity with the corresponding domain of the well-characterized NAGases. The recombinant NAGaseA had a molecular mass of 92 kDa. Biochemical characterization of the purified NAGaseA revealed that the optimal reaction condition was at 40°C and pH 6.5, and exhibited great pH stability in the range of pH 6.5–9.5. The Vmax, Km, Kcat, and Kcat/Km of NAGaseA toward pNP-GlcNAc were 3333.33 µmol min−1 L−1, 39.99 µM, 4667.07 s−1, and 116.71 mL µmol −1 s−1, respectively. Analysis of the hydrolysis products of N-acetyl chitinoligosaccharides (N-Acetyl COSs) indicated that NAGaseA capable of converting N-Acetyl COSs((GlcNAc)2–(GlcNAc)6) into GlcNAc with hydrolysis ability order: (GlcNAc)2 > (GlcNAc)3 > (GlcNAc)4 > (GlcNAc)5 > (GlcNAc)6. Moreover, NAGaseA could generate (GlcNAc)3–(GlcNAc)6 from (GlcNAc)2–(GlcNAc)5, respectively. These results showed that NAGaseA is a multi-functional NAGase with transglycosylation activity. In addition, significantly synergistic action was observed between NAGaseA and other sources of chitinases during hydrolysis of colloid chitin. Finally, 0.759 g/L, 0.481 g/L and 0.986 g/L of GlcNAc with purity of 96% were obtained using different chitinase combinations, resulting in 1.6-2.69 fold increase, respectively.
Conclusions

The hydrolytic properties and good environmental adaptions indicate that NAGaseA has great potential in the bioconversion of chitin waste and behaved as an excellent candidate in GlcNAc producing.
Research plan for evaluating service quality of public art museums through on-site survey using IAM scale of three question groups
Authors: Iwakiri Momone , Moteki Yasutoshi 
Published: January 2022

Abstract
We examine the methods for evaluating service quality in public art museums. Specifically, we will conduct a multi-group questionnaire survey of museum users on their satisfaction with the services provided by public museums. Using selective multigroup principal component regression analysis—which combines principal component analysis and multiple regression analysis—we will statistically examine the factors that public art museums should emphasize when providing services to their users. The four-page questionnaire will comprise three groups: hardware (building and interior), software (staff responses), and exhibition content. Each question will be rated on a 7-point Likert scale. We named the survey scale the indicators for art museums’ service quality scale. The scale comprises three question groups. The eventual survey will be conducted at the Higashihiroshima City Museum of Art for six business days on the third week of August 2022. Self-administered questionnaires will be distributed to visitors to the special exhibition. We are disclosing our research methods and data analysis procedures prior to conducting the research to prevent hypothesizing after the results are known, and to ensure the transparency of the research. In addition, presenting the details of the research implementation and data analysis methods can promote the accuracy of follow-up and replication experiments. Our proposed scale has the following advantages. First, it is simpler than the existing SERVQUAL-based survey framework, which is a 5-group scale, and is expected to reduce the burden on respondents and increase the response rate in actual surveys. Second, the fact that the opinions of those in charge of art museums were heard from the very beginning of the formulation of the research framework, makes the explanatory power of the analytical model, as expressed in the coefficient of determination of the multiple regression analysis, high for a scale targeting public art museums.
Validation of A New Classification System For Surgical Planning of Vertebral Body Tethering (VBT)
Authors: Trobisch Per D , Birkenmaier Christof , Migliorini Filippo , Bas Teresa , Berjano Pedro , Cho Samuel K , Courvoisier Aurelien , Hegde Sajan , Helenius Ilkka J , Lui Darren F , Papadopoulos Elias C , Maffulli Nicola , Baroncini Alice 
Published: January 2022

Abstract
BACKGROUND: Vertebral body tethering (VBT) may represent a paradigm shift in the management of adolescent idiopathic scoliosis (AIS). However, the ideal candidates, who may profit the most from this technique, have not yet been identified. We evaluated patients based on their curve type and on the potential benefits of VBT over fusion and developed a new classification to guide decision making for VBT, evaluating 5 different curve types (lumbar, double, long thoracic, short thoracic, presence of high thoracic curve). This study analyzed the inter- and intrarater variability of this new classification. METHODS: 11 spinal deformity surgeons contributed to the validation. Four radiographs (p. a., lateral, right and left p. a. side bending) were prepared for 27 patients. Two sets of the patients’ images were sent to each participant, with identical radiographs but in different order. Each participant was given instructions on how to employ the classification and was asked to apply it to the radiographs. The inter- and intra-observer reliability was assessed evaluating the kappa coefficient (k).RESULTS: The overall inter-observer reliability was k = 0.84 (confidence interval: 0.76 to 0.91). The overall intra-observer reliability was k = 0.88 (confidence interval 95%: 0.68 to 1.00). All k values were statistically significant and indicated an excellent inter- and intra-observer reliability of the classification.CONCLUSION: The newly developed classification for VBT planning showed an excellent reliability.
Wide bandgap semiconductor nanomembranes as a long-term bio-interface for flexible, implanted neuromodulator
Authors: Nguyen Tuan-Khoa , Barton Matthew , Ashok Aditya , Truong Thanh-An , Yadav Sharda , Leitch Michael , Nguyen Thanh-Vinh , Kashaninejad Navid , Dinh Toan , Hold Leonie , Yamauchi Yusuke , Nguyen Nam-Trung , Phan Hoang-Phuong 
Published: January 2022

Abstract
Electrical neuron stimulation holds promise for the treatment of several chronic neurological disorders, including spinal cord injury, epilepsy, and Parkinson’s disease. The implementation of ultrathin, flexible electrodes, that can offer non-invasive attachment to soft neural tissues, is a breakthrough technology for timely, continuous, programable, and spatial stimulations. However, to enable flexibility in neural electrodes, the conventional thick and bulky ceramic package is no longer applicable to soft electronics, which poses several technical issues such as device degradation and long-term stability. We introduce herein a new concept of long-lived flexible neural electrodes using silicon carbide nanomembranes as the Faradaic interface, and thermal oxide thin films as the electrical isolation layer. The silicon carbide (SiC) membranes were developed using a wafer-level chemical deposition process while thermal oxide was grown employing a standard and high-quality wet oxidation approach, which are scalable and compatible with industrial microelectronic technologies. Our experimental results showed excellent stability in the SiC/SiO2 hybrid system that can potentially last several decades with maintained reliable electrical properties in biofluid environments. We demonstrated the capability of our material system in stimulating peripheral nerves (i.e., sciatic nerve) in rat models, showing comparable muscle contraction response recorded from electromyogram (EMG) stimulation results to a gold-standard non-implanted nerve stimulation device. The design concept, scalable fabrication approach, and the multimodal functionalities in SiC/SiO2 flexible electronics open an exciting possibility for fundamental neuroscience studies as well as clinical neural stimulation-based therapy.
Eradicating little fire ants (Wasmannia auropunctata) from the island of Kaua`i, Hawai`i: process, challenges, and results to date
Authors: Montgomery Michelle , Vanderwoude Casper , Lynch A. J. Jasmyn , Keanini Tiffani , Kaneshige Craig 
Published: January 2022

Abstract
First detected on the Island of Kaua`i in 1999, the little fire ant (Wasmannia auropunctata) has persisted despite early attempts at eradication by state agencies. A multi-agency collaboration was formed in 2011 to develop and implement a two-phase eradication plan. The infestation was delimited to 4.02 ha of rural residences and steep coastline habitat. Treatments regimens were divided between easily accessible infested areas (Phase I) and steep areas requiring rope safety systems (Phase II). The eradication plan included ground and arboreal treatment technologies and long-term post-treatment monitoring. Treatments consisted of broadcasting baits containing s-methoprene (0.25% a.i.), indocaxarb (0.18% a.i), or hydramethylnon (0.73% a.i.) eight times over the course of 12 months. Treatments effectively reduced the population to below detectable levels throughout much of the treatment area, however isolated remnant colonies were detected as recently as September 2019. Remnant colonies were associated with specific site features known to be difficult to treat, such as tall palm trees and large mulch piles. On one occasion, in 2017, two little fire ant detections were confirmed outside of the original treatment area, where no little fire ants had been detected before; highlighting the ability for little fire ants to remain undetected for many years. Current results stress the importance of long-term commitment to post-treatment monitoring and expanded surveys. We recommend development and incorporation of novel detection methods which could increase accuracy and improve efficiency of monitoring and detection surveys.
State of art bio-materials as scale inhibitors in recirculating cooling water system: a review article
Authors: Hu Yanglin , Chen Chuanmin , Liu Songtao 
Published: January 2022

Abstract
During the operation of the circulating cooling water system, inorganic scale deposition may cause technical problems, such as reduction of heat transfer efficiency in cooling systems and obstruction of pipes. In the industry, chemicals are often used as scale inhibitors in scale deposition control, antiscalants popular in industry are generally phosphorus and nitrogen-containing chemicals, which may lead to eutrophication. However, increasing environmental concern and discharge limitations have guided antiscalants to move toward biodegradability, nontoxicity and cost–effectiveness. This paper reviews current research on the application of using bio-materials as scale inhibitors, including proteins and amino acids, polysaccharides, plant extracts, microbial reagents, and microbiological products. The non-bioaccumulation, low cost, readily biodegradability and sustainably available characters promote the development of green-scale inhibitor chemistry.
Natural Products Against COVID-19 Inflammation: A Mini-Review
Authors: Scotti Luciana , Lopes Simone Mendes , de Medeiros Herbert Igor Rodrigues , Scotti Marcus Tullius 
Published: January 2022

Abstract:
Severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) is a virus whose genetic
material is positive single-stranded RNA, being responsible for coronavirus disease 2019 (COVID-
19), an infection that compromises the lungs and consequently the respiratory capacity of the infected
individual, according to the WHO in November 2021, 249,743,428 cases were confirmed, of
which 5,047,652 individuals died due to complications resulting from the infection caused by SARSCOV-
2. As the infection progresses, the individual may experience loss of smell and taste, as well as
breathing difficulties, severe respiratory failure, multiple organ failure, and death. Due to this new
epidemiological agent in March 2020 it was announced by the director general of the World Health
Organization (WHO) a pandemic status, and with that, many research groups are looking for new therapeutic
alternatives through synthetic and natural bioactives. This research is a literature review of some
in silico studies involving natural products against COVID-19 inflammation published in 2020 and
2021. Work like this presents relevant information to the scientific community, boosting future research
and encouraging the use of natural products for the search for new antivirals against COVID-19.
GAAP: A GUI-based Genome Assembly and Annotation Package
Authors: Singla Deepak , Yadav Inderjit Singh 
Published: January 2022

Background:
Next-generation sequencing (NGS) technologies are being continuously
used for high-throughput sequencing data generation that requires easy-to-use GUI-based data analysis
software. These kinds of software could be used in-parallel with sequencing for the automatic
data analysis. At present, very few software are available for use and most of them are commercial,
thus creating a gap between data generation and data analysis.

Method:
GAAP is developed on the NodeJS platform that uses HTML, JavaScript as the front-
end for communication with users. We have implemented FastQC and trimmomatic tool for quality
checking and control. Velvet and Prodigal are integrated for genome assembly and gene prediction.
The annotation will be done with the help of remote NCBI Blast and IPR-Scan. In the backend,
we have used PERL and JavaScript for the processing of data. To evaluate the performance of
GAAP, we have assembled a viral (SRR11621811), bacterial (SRR17153353) and human genome
(SRR16845439).

Result:
We have used GAAP software to assemble, and annotate a COVID-19 genome on a desktop
computer that resulted in a single contig of 27994bp with 99.57% reference genome coverage.
This assembly predicted 11 genes, of which 10 were annotated using annotation module of GAAP.
We have also assembled a bacterial and human genome 138 and 194281 contigs with N50 value
100399 and 610, respectively.

Conclusion:
In this study, we have developed freely available, platform-independent genome assembly
and annotation (GAAP) software (www.deepaklab.com/gaap). The software itself acts as a
complete data analysis package with quality check, quality control, de-novo genome assembly,
gene prediction and annotation (Blast, PFAM, GO-Term, pathway and enzyme mapping) modules.
Interleukin Receptor Antagonists and Janus Kinase Inhibitors Repurposed
for Treatment of COVID-19
Authors: Tandon Runjhun , Paul Kamaldeep , Tandon Nitin , Luxami Vijay 
Published: January 2022

Abstract:
SARS-CoV-2 infection is the most contagious among the three coronavirus infections the
world has witnessed to date, which has affected almost all parts of the world in millions of population
since its outbreak in China in December 2019. Moreover, it has severely hit the world economy and
therefore there is a dire need to develop the treatment of this deadly disease. A number of potential
vaccines are in the early or advanced stage of clinical trials. But the development of a vaccine is a
very tedious and time-consuming task. Therefore, various groups are working on repurposing of drugs
with already known safety and efficacy profiles to shorten the time of development of the potential
treatment. The main aim of this review article is to summarize the clinical outcomes of Interleukin
receptor antagonists and Janus kinase inhibitors based drugs which have been repurposed for the
treatment of COVID-19 associated with SARS-CoV-2.
(Pro)renin Receptor and Blood Pressure Regulation: A Focus on the Central
Nervous System
Authors: Earley Yumei Feng , Souza Lucas A.C. 
Published: January 2022

Abstract:
The renin-angiotensin system (RAS) is classically described as a hormonal system in
which angiotensin II (Ang II) is one of the main active peptides. The action of circulating Ang II
on its cognate Ang II type-1 receptor (AT1R) in circumventricular organs has important roles in regulating
the autonomic nervous system, blood pressure (BP) and body fluid homeostasis, and has
more recently been implicated in cardiovascular metabolism. The presence of a local or tissue RAS
in various tissues, including the central nervous system (CNS), is well established. However, because
the level of renin, the rate-limiting enzyme in the systemic RAS, is very low in the brain,
how endogenous angiotensin peptides are generated in the CNS—the focus of this review—has
been the subject of considerable debate. Notable in this context is the identification of the
(pro)renin receptor (PRR) as a key component of the brain RAS in the production of Ang II in the
CNS. In this review, we highlight cellular and anatomical locations of the PRR in the CNS. We also
summarize studies using gain- and loss-of function approaches to elucidate the functional importance
of brain PRR-mediated Ang II formation and brain RAS activation, as well as PRR-mediated
Ang II-independent signaling pathways, in regulating BP. We further discuss recent developments
in PRR involvement in cardiovascular and metabolic diseases and present perspectives for future directions.