bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding complex genetic architecture of rice grain weight through QTL-meta analysis and candidate gene identification
Authors: Anilkumar C. , Sah Rameswar Prasad , Muhammed Azharudheen T. P. , Behera Sasmita , Singh Namita , Prakash Nitish Ranjan , Sunitha N. C. , Devanna B. N. , Marndi B. C. , Patra B. C. , Nair Sunil Kumar 
Published: August 2022

AbstractQuantitative trait loci (QTL) for rice grain weight identified using bi-parental populations in various environments were found inconsistent and have a modest role in marker assisted breeding and map-based cloning programs. Thus, the identification of a consistent consensus QTL region across populations is critical to deploy in marker aided breeding programs. Using the QTL meta-analysis technique, we collated rice grain weight QTL information from numerous studies done across populations and in diverse environments to find constitutive QTL for grain weight. Using information from 114 original QTL in meta-analysis, we discovered three significant Meta-QTL (MQTL) for grain weight on chromosome 3. According to gene ontology, these three MQTL have 179 genes, 25 of which have roles in developmental functions. Amino acid sequence BLAST of these genes indicated their orthologue conservation among core cereals with similar functions. MQTL3.1 includes the OsAPX1, PDIL, SAUR, and OsASN1 genes, which are involved in grain development and have been discovered to play a key role in asparagine biosynthesis and metabolism, which is crucial for source-sink regulation. Five potential candidate genes were identified and their expression analysis indicated a significant role in early grain development. The gene sequence information retrieved from the 3 K rice genome project revealed the deletion of six bases coding for serine and alanine in the last exon of OsASN1 led to an interruption in the synthesis of α-helix of the protein, which negatively affected the asparagine biosynthesis pathway in the low grain weight genotypes. Further, the MQTL3.1 was validated using linked marker RM7197 on a set of genotypes with extreme phenotypes. MQTL that have been identified and validated in our study have significant scope in MAS breeding and map-based cloning programs for improving rice grain weight.
High-throughput barcoding method for the genetic surveillance of insecticide resistance and species identification in Anopheles gambiae complex malaria vectors
Authors: Campos Monica , Phelan Jody , Spadar Anton , Collins Emma , Gonçalves Adéritow , Pelloquin Bethanie , Vaselli Natasha Marcella , Meiwald Anne , Clark Emma , Stica Caleb , Orsborne James , Sylla Moussa , Edi Constant , Camara Denka , Mohammed Abdul Rahim , Afrane Yaw Asare , Kristan Mojca , Walker Thomas , Gomez Lara Ferrero , Messenger Louisa A. , Clark Taane G. , Campino Susana 
Published: August 2022

AbstractSurveillance of malaria vector species and the monitoring of insecticide resistance are essential to inform malaria control strategies and support the reduction of infections and disease. Genetic barcoding of mosquitoes is a useful tool to assist the high-throughput surveillance of insecticide resistance, discriminate between sibling species and to detect the presence of Plasmodium infections. In this study, we combined multiplex PCR, custom designed dual indexing, and Illumina next generation sequencing for high throughput single nucleotide polymorphism (SNP)-profiling of four species from the Anopheles (An.) gambiae complex (An. gambiae sensu stricto, An. coluzzii, An. arabiensis and An. melas). By amplifying and sequencing only 14 genetic fragments (500 bp each), we were able to simultaneously detect Plasmodium infection; insecticide resistance-conferring SNPs in ace1, gste2, vgsc and rdl genes; the partial sequences of nuclear ribosomal internal transcribed spacers (ITS1 and ITS2) and intergenic spacers (IGS), Short INterspersed Elements (SINE), as well as mitochondrial genes (cox1 and nd4) for species identification and genetic diversity. Using this amplicon sequencing approach with the four selected An. gambiae complex species, we identified a total of 15 non-synonymous mutations in the insecticide target genes, including previously described mutations associated with resistance and two new mutations (F1525L in vgsc and D148E in gste2). Overall, we present a reliable and cost-effective high-throughput panel for surveillance of An. gambiae complex mosquitoes in malaria endemic regions.
Pandemic policy assessment by artificial intelligence
Authors: Song Sirui , Liu Xue , Li Yong , Yu Yang 
Published: August 2022

AbstractMobility-control policy is a controversial nonpharmacological approach to pandemic control due to its restriction on people’s liberty and economic impacts. Due to the computational complexity of mobility control, it is challenging to assess or compare alternative policies. Here, we develop a pandemic policy assessment system that employs artificial intelligence (AI) to evaluate and analyze mobility-control policies. The system includes three components: (1) a general simulation framework that models different policies to comparable network-flow control problems; (2) a reinforcement-learning (RL) oracle to explore the upper-bound execution results of policies; and (3) comprehensive protocols for converting the RL results to policy-assessment measures, including execution complexity, effectiveness, cost and benefit, and risk. We applied the system to real-world metropolitan data and evaluated three popular policies: city lockdown, community quarantine, and route management. For each policy, we generated mobility-pandemic trade-off frontiers. The results manifest that the smartest policies, such as route management, have high execution complexity but limited additional gain from mobility retention. In contrast, a moderate-level intelligent policy such as community quarantine has acceptable execution complexity but can effectively suppress infections and largely mitigate mobility interventions. The frontiers also show one or two turning points, reflecting the safe threshold of mobility retention when considering policy-execution errors. In addition, we simulated different policy environments and found inspirations for the current policy debates on the zero-COVID policy, vaccination policy, and relaxing restrictions.
Heterogeneous rarity patterns drive price dynamics in NFT collections
Authors: Mekacher Amin , Bracci Alberto , Nadini Matthieu , Martino Mauro , Alessandretti Laura , Aiello Luca Maria , Baronchelli Andrea 
Published: August 2022

AbstractWe quantify Non Fungible Token (NFT) rarity and investigate how it impacts market behaviour by analysing a dataset of 3.7M transactions collected between January 2018 and June 2022, involving 1.4M NFTs distributed across 410 collections. First, we consider the rarity of an NFT based on the set of human-readable attributes it possesses and show that most collections present heterogeneous rarity patterns, with few rare NFTs and a large number of more common ones. Then, we analyze market performance and show that, on average, rarer NFTs: (i) sell for higher prices, (ii) are traded less frequently, (iii) guarantee higher returns on investment, and (iv) are less risky, i.e., less prone to yield negative returns. We anticipate that these findings will be of interest to researchers as well as NFT creators, collectors, and traders.
CMOS based capacitive sensor matrix for characterizing and tracking of biological cells
Authors: Abdelbaset Reda , El-Sehrawy Yehia , Morsy Omar E. , Ghallab Yehya H. , Ismail Yehea 
Published: August 2022

AbstractThe characterization and tracking of biological cells using biosensors are necessary for many scientific fields, specifically cell culture monitoring. Capacitive sensors offer a great solution due to their ability to extract many features such as the biological cells' position, shape, and capacitance. Through this study, a CMOS-based biochip that consists of a matrix of capacitive sensors (CSM), utilizing a ring oscillator-based pixel readout circuit (PRC), is designed and simulated to track and characterize a single biological cell based on its aforementioned different features. The proposed biochip is simulated to characterize a single Hepatocellular carcinoma cell (HCC) and a single normal liver cell (NLC). COMSOL Multiphysics was used to extract the capacitance values of the HCC and NLC and test the CSM's performance at different distances from the analyte. The PRC's ability to detect the extracted capacitance values of the HCC and NLC is evaluated using Virtuoso Analog Design Environment. A novel algorithm is developed to animate and predict the location and shape of the tested biological cell depending on CSM's capacitance readings simultaneously using MATLAB R2022a script. The results of both models, the measured capacitance from CSM and the correlated frequency from the readout circuit, show the biochip's ability to characterize and distinguish between HCC and NLC.
A new mixture copula model for spatially correlated multiple variables with an environmental application
Authors: Abraj Mohomed , Wang You-Gan , Thompson M. Helen 
Published: August 2022

AbstractIn environmental monitoring, multiple spatial variables are often sampled at a geographical location that can depend on each other in complex ways, such as non-linear and non-Gaussian spatial dependence. We propose a new mixture copula model that can capture those complex relationships of spatially correlated multiple variables and predict univariate variables while considering the multivariate spatial relationship. The proposed method is demonstrated using an environmental application and compared with three existing methods. Firstly, improvement in the prediction of individual variables by utilising multivariate spatial copula compares to the existing univariate pair copula method. Secondly, performance in prediction by utilising mixture copula in the multivariate spatial copula framework compares with an existing multivariate spatial copula model that uses a non-linear principal component analysis. Lastly, improvement in the prediction of individual variables by utilising the non-linear non-Gaussian multivariate spatial copula model compares to the linear Gaussian multivariate cokriging model. The results show that the proposed spatial mixture copula model outperforms the existing methods in the cross-validation of actual and predicted values at the sampled locations.
Concentration-dependent effects of effusol and juncusol from Juncus compressus on seedling development of Arabidopsis thaliana
Authors: Bakacsy László , Sípos Lilla , Barta Anita , Stefkó Dóra , Vasas Andrea , Szepesi Ágnes 
Published: August 2022

AbstractJuncus species are valuable sources of phenanthrene compounds that have been used in traditional Chinese medicine for thousands of years. Effusol and juncusol are the most investigated compounds reported to have antimicrobial and anticancer effects; however, to date, their effects on higher plants have not been investigated. In this study, we examined the effects of effusol and juncusol on the growth and other biochemical parameters of the dicot model plant Arabidopsis thaliana in a concentration-dependent manner with a focus on polyamine metabolism. Phenanthrene induced toxic effects on plant growth and development, while effusol and juncusol induced higher biomass and maintained antioxidant defence mechanisms associated with reduced polyamine degradation. Taken together, our results suggest that these compounds could be good candidates for new biopesticide or biostimulant plant growth regulators in the future.
Allopatric molting of Devonian trilobites
Authors: Zong Ruiwen , Gong Yiming 
Published: August 2022

AbstractTrilobite exuviae record the development of individual trilobites and their molting process and can also contain information on their behavior. The silt- to fine-grained tuffites of the middle part of the Middle Member of the Upper Devonian Hongguleleng Formation in western Junggar contains abundant phacopidae trilobite, specificallyOmegopssp. A, almost all of which are exuviae. Based on the preservation pattern, burial environment, and set of organisms co-occurring withOmegopssp. A, we speculate that the environment represented by the middle part of the Middle Member of the Hongguleleng Formation served only as the molting site ofOmegopssp. A, and that their primary habitat was elsewhere.Omegopssp. A would have thus travelled to deep-water to molt. The reasons for allopatric molting may have included avoiding predators and interference from competing organisms during molting. This implies that the migratory behavior of some modern arthropods may have existed since at least the Devonian. This behavior suggests that Late Devonian phacopidae trilobites may have migrated to deeper water expanded their ecological domain as a survival strategy in response to unfavorable ecological environment.
Differences in motor imagery strategy change behavioral outcome
Authors: Fukumoto Yuki , Todo Marina , Bunno Yoshibumi , Yoneda Hirohisa , Tani Makiko , Suzuki Toshiaki 
Published: August 2022

AbstractKinesthetic motor imagery (KMI) involves imagining the feeling and experience of movements. We examined the effects of KMI, number visualizing, and KMI with number visualizing on the excitability of spinal motor neurons and a behavioral outcome measure in a pinch force task. Healthy participants (13 men and 8 women; mean age: 24.8 ± 5.5 years) were recruited. We compared the F-waves of the left thenar muscles after stimulating the left median nerve at the wrist during each motor imagery condition after a practice session. The KMI condition consisted of imagining muscle contraction, the number visualizing condition consisted of imagining the pinch force increasing numerically, and the KMI with number visualizing consisted of alternating between the KMI and imagining the pinch force increasing numerically. Before and after motor imagery, the time required to adjust to the target pinch force was compared. The time required to adjust the pinch force was shorter in the KMI with number visualizing condition than in the KMI and number visualizing conditions. There was no difference in the F/M amplitude ratio between each MI strategy condition, indicating the excitability of spinal motor neurons. Numerical information helped to improve the ability of participants to perform KMI.
Improving taste sensitivity in healthy adults using taste recall training: a randomized controlled trial
Authors: Otsubo Yuta , Miyagi Midori , Sekiya Hideki , Kano Osamu , Ebihara Satoru 
Published: August 2022

AbstractAlthough many patients suffer from taste disorder, methods to improve taste sensitivity are limited. To develop a taste recall training method to improve the perception of taste, 42 healthy individuals were randomly assigned to either the training or the control group. Using the filter paper disc method, participants in the training group were asked to match the four tastes (sweetness, saltiness, sourness, and bitterness) between those of taste recognition thresholds and those of a one-step higher concentration until they get them right. Then, they were asked to match the four tastes between those of one-step lower and one-step higher in concentration from their taste recognition thresholds until they get them right. Finally, they were asked to match the four tastes between those of one-step lower concentration and those of their taste recognition thresholds until they get them right. This training was repeated until perfectly matched. The taste recall training program led to a lowered taste recognition threshold in healthy adults for each taste quality, suggesting the improvement of taste sensitivity. This lowered threshold for each taste was observed with each additional training session. We conclude that this taste recall training method might be a therapeutic approach for treating taste disorder.
Free-living and particle-attached bacterial community composition, assembly processes and determinants across spatiotemporal scales in a macrotidal temperate estuary
Authors: Urvoy Marion , Gourmelon Michèle , Serghine Joëlle , Rabiller Emilie , L’Helguen Stéphane , Labry Claire 
Published: August 2022

AbstractBacteria play an important role in biogeochemical cycles as they transform and remineralize organic matter. Particles are notable hotspots of activity, hosting particle-attached (PA) communities that can differ largely from their free-living (FL) counterparts. However, long-standing questions remain concerning bacterial community assembly processes and driving factors. This study investigated the FL and PA community compositions and determinants within the Aulne estuary and the Bay of Brest coastal waters (France). Our results revealed that the FL and PA community compositions greatly varied with salinity and season, explaining a larger part of the variance than the sampling fraction. Both the FL and PA communities were driven by deterministic assembly processes and impacted by similar factors. The FL-PA dissimilarity varied across space and time. It decreased in the estuarine stations compared to the freshwater and marine ends, and in summer. Interestingly, a significant proportion of the FL and PA communities' β-diversity and dissimilarity was explained by cohesion, measuring the degree of taxa co-occurrence. This suggested the importance of co-occurrence patterns in shaping the FL and PA community compositions. Our results shed light on the factors influencing estuarine bacterial communities and provide a first step toward understanding their biogeochemical impacts.
Healthy worker survival effect at a high-altitude mine: prospective cohort observation
Authors: Vinnikov Denis , Krasotski Viktor 
Published: August 2022

AbstractVery little is known about the factors of healthy worker survival effect at high-altitude mines. We conducted this cohort observation of the new hires for a high-altitude gold mine in Kyrgyzstan with the aim to ascertain predictors of survival at work. All new hires in 2009 through 2012 for a high-altitude gold mine (3600–4500 m above sea level) were followed up to January 2022. We tested the association of demographic, physiological predictors and diagnoses at the pre-employment screening with non-survival at work in Cox proportional hazards yielding hazard ratios (HR) with their 95% confidence intervals (CI). The cumulative observation time was 5190 person-years. Blood pressure at pre-employment, lung function, the diagnoses of essential hypertension, chronic obstructive pulmonary disease (COPD) or any other analyzed physiological variables were not associated with non-survival at work. However, smoking (HR 1.55; 95% CI 1.10; 2.17) increased the likelihood of non-survival at work, independent of any diagnosis or lowland residence (HR 1.95; 95% CI 1.31; 2.90). Adjusted for covariates and all diagnoses, having chronic rheumatic fever (HR 10.95; 95% CI 2.92; 33.92), hemorrhoids (HR 1.32; 95% CI 1.01; 3.75), adhesive otitis (HR 1.74; 95% CI 1.05; 2.89) or obesity (HR 1.71; 95% CI 1.01; 2.88) were associated with non-survival at work with time. This prospective observation of new hires for a high-altitude mining operation demonstrated that selected diagnoses, smoking and lowland residence elevated the risk of early exit in prospective workers.
Receptor for advanced glycation end-products (RAGE) mediates phagocytosis in nonprofessional phagocytes
Authors: Yang Yan , Liu Guoyu , Li Feng , Carey Lucas B. , Sun Changjin , Ling Kaiping , Tachikawa Hiroyuki , Fujita Morihisa , Gao Xiao-Dong , Nakanishi Hideki 
Published: August 2022

AbstractIn mammals, both professional phagocytes and nonprofessional phagocytes (NPPs) can perform phagocytosis. However, limited targets are phagocytosed by NPPs, and thus, the mechanism remains unclear. We find that spores of the yeast Saccharomyces cerevisiae are internalized efficiently by NPPs. Analyses of this phenomenon reveals that RNA fragments derived from cytosolic RNA species are attached to the spore wall, and these fragments serve as ligands to induce spore internalization. Furthermore, we show that a multiligand receptor, RAGE (receptor for advanced glycation end-products), mediates phagocytosis in NPPs. RAGE-mediated phagocytosis is not uniquely induced by spores but is an intrinsic mechanism by which NPPs internalize macromolecules containing RAGE ligands. In fact, artificial particles labeled with polynucleotides, HMGB1, or histone (but not bovine serum albumin) are internalized in NPPs. Our findings provide insight into the molecular basis of phagocytosis by NPPs, a process by which a variety of macromolecules are targeted for internalization.
Identity-Focused Practice in Augmentative and Alternative Communication Services: A Framework to Support the Intersecting Identities of Individuals With Severe Disabilities
Authors: Wofford Mary Claire , Ogletree Billy T. , De Nardo Thales 
Published: August 2022

Purpose:The purpose of this clinical focus article is to consider a framework of identity in provision of services to individuals with severe disabilities who use augmentative and alternative communication (AAC).Method:Components of the framework for identity include personal, social, and cultural areas. These aspects are identified in extant literature on AAC services and discussed through the lens of the Communication Bill of Rights (National Joint Committee for the Communication Needs of Persons with Severe Disabilities in 1992). Identity-focused practice is discussed as being relevant to self-determination and quality of life among individuals with severe disabilities.Results:A case study illustrates identity-focused practice in delivery of services to an adolescent who uses AAC, considering personal, social, and cultural identities.Conclusions:This clinical focus article proposes a theoretical framework and provides a practice example for addressing the intersecting identities of individuals with severe disabilities. Furthermore, this clinical focus article argues that identity is a basic human right and area in which additional efforts are needed in the application of AAC solutions.