bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of recombinant humanized collagen type III and its influence on cell behavior and phenotype
Authors: Wang Jing , Hu Hong , Wang Jian , Qiu He , Gao Yongli , Xu Yang , Liu Zhanhong , Tang Yajun , Song Lu , Ramshaw John , Lin Hai , Zhang Xingdong 
Published: December 2022

AbstractCollagen made a tremendous impact in the field of regenerative medicine as a bioactive material. For decades, collagen has been used not only as a scaffolding material but also as an active component in regulating cells' biological behavior and phenotype. However, animal-derived collagen as a major source suffered from problems of immunogenicity, risk of viral infection, and the unclear relationship between bioactive sequence and function. Recombinant humanized collagen (rhCol) provided alternatives for regenerative medicine with more controllable risks. However, the characterization of rhCol and the interaction between rhCol and cells still need further investigation, including cell behavior and phenotype. The current study preliminarily demonstrated that recombinant humanized collagen type III (rhCol III) conformed to the theoretical amino acid sequence and had an advanced structure resembling bovine collagen. Furthermore, rhCol III could facilitate basal biological behaviors of human skin fibroblasts, such as adhesion, proliferation and migration. rhCol III was beneficial for some extracellular matrix-expressing cell phenotypes. The study would shed light on the mechanism research of rhCol and cell interactions and further understanding of effectiveness in tissue regeneration.

Graphical abstract
Connecting for Care: a protocol for a mixed-method social network analysis to advance knowledge translation in the field of child development and rehabilitation
Authors: Glegg Stephanie , Costello Carrie , Barnaby Symbia , Cassidy Christine , Sibley Kathryn M. , Russell Kelly , Kingsnorth Shauna , Pritchard Lesley , de Camargo Olaf Kraus , Andersen John , Bellefeuille Samantha , Cross Andrea , Curran Janet , Hesketh Kim , Layco Jeremy , Reynolds James , Robeson Paula , Straus Sharon , Wittmeier Kristy 
Published: December 2022

AbstractBackgroundConnections between individuals and organizations can impact knowledge translation (KT). This finding has led to growing interest in the study of social networks as drivers of KT. Social networks are formed by the patterns of relationships or connections generated through interactions. These connections can be studied using social network analysis (SNA) methodologies. The relatively small yet diverse community in the field of child development and rehabilitation (CD&R) in Canada offers an ideal case study for applying SNA. The purposes of this work are to (1) quantify and map the structure of Canadian CD&R KT networks among four groups: families, health care providers, KT support personnel, and researchers; (2) explore participant perspectives of the network structure and of KT barriers and facilitators within it; and (3) generate recommendations to improve KT capacity within and between groups. Aligning with the principles of integrated KT, we have assembled a national team whose members contribute throughout the research and KT process, with representation from the four participant groups.MethodsA sequential, explanatory mixed-method study, within the bounds of a national case study in the field of CD&R. Objective 1: A national SNA survey of family members with advocacy/partnership experience, health care providers, KT support personnel, and researchers, paired with an anonymous survey for family member without partnership experience, will gather data to describe the KT networks within and between groups and identify barriers and facilitators of network connections. Objective 2: Purposive sampling from Phase 1 will identify semi-structured interview participants with whom to examine conventional and network-driven KT barriers, facilitators, and mitigating strategies. Objective 3: Intervention mapping and a Delphi process will generate recommendations for network and conventional interventions to strengthen the network and facilitate KT.DiscussionThis study will integrate network and KT theory in mapping the structure of the CD&R KT network, enhance our understanding of conventional and network-focused KT barriers and facilitators, and provide recommendations to strengthen KT networks. Recommendations can be applied and tested within the field of CD&R to improve KT, with the aim of ensuring children achieve the best health outcomes possible through timely access to effective healthcare.
A case of bronchial atresia treated with right S6 segmentectomy using fluorescence navigation with indocyanine green
Authors: Watanabe Mototsugu , Kondo Kaoru , Furukawa Shinichi , Shiotani Toshio , Kataoka Kazuhiko 
Published: December 2022

Abstract
Background
Bronchial atresia is a congenital obstruction of the segmental or lobar bronchus that often leads to hyperinflation of the affected area. It can cause intractable infections and abnormal nodules in these regions and surgical resection needs to be considered. The precise resection of the abnormal pulmonary segment is crucial for diagnosis and treatment.

Case presentation
A 24-year-old male patient was incidentally diagnosed with an abnormal lung shadow, which was confirmed to be bronchial atresia with computerized tomography imaging and bronchofiberscopy. Segmentectomy using fluorescence navigation with indocyanine green dye was performed. The patient had no trouble in his clinical course, indicating that this procedure may be useful in the resection of congenital bronchial atresia regions.

Conclusion
Segmentectomy using indocyanine green is an appropriate technique for minimal resection without respiratory impairment and diagnostic therapy of bronchial atresia.
Efficacy, Safety, and Patient-Reported Outcomes of Atezolizumab Plus Bevacizumab for Unresectable Hepatocellular Carcinoma in Thailand: A Multicenter Prospective Study
Authors: Charonpongsuntorn Chanchai , Tanasanvimon Suebpong , Korphaisarn Krittiya , Payapwattanawong Songwit , Siripoon Teerada , Pakvisal Nussara , Juengsamarn Jitlada , Phaibulvatanapong Ekkamol , Chindaprasirt Jarin , Prasongsook Naiyarat , Udomdamrongkul Kittipong , Ngamphaiboon Nuttapong , Sirachainan Ekaphop 
Published: December 2022

PURPOSEAtezolizumab plus bevacizumab treatment is a first-line therapy for unresectable hepatocellular carcinoma (HCC) worldwide. The efficacy, safety, and patient-reported outcomes (PROs) of HCC in Thailand have not yet been reported. This study aimed to evaluate the efficacy, safety, and PROs of atezolizumab plus bevacizumab.MATERIALS AND METHODSFrom September 2020 to August 2021, 30 patients with unresectable HCC who met the inclusion criteria of atezolizumab plus bevacizumab as first-line treatment were enrolled. Analysis was assessed for progression-free survival, overall survival, adverse events (AEs), and quality of life (QoL).RESULTSThe median progression-free survival and overall survival periods were 6.7 and 10.2 months, respectively. The disease control rate was 63.3%. The frequent AEs were proteinuria, hypertension, and hepatitis. Serious AEs included gastrointestinal bleeding, but none of the patients died from serious AEs. The discontinuation rate was 23.3%, and the median number of treatment cycles was 10.5 cycles. In total, 23.3% of the patients continued treatment after 1 year of therapy. The global health status/QoL and physical function scores showed less deterioration at baseline than at 3 and 6 months (median scores = 76.7, 71.6, and 64.1 in QoL and 84.7, 79.6, and 79.0 in physical function, respectively). The HCC18 symptom score index data showed a slow progression of symptom scores from baseline to 3 and 6 months (12.7, 19.6, and 22.3, respectively).CONCLUSIONThis study demonstrates that atezolizumab plus bevacizumab is effective and has a safety profile comparable with that of previous studies as first-line therapy for unresectable HCC in a real-world setting and in Thai populations. Data on PROs also demonstrate benefits in terms of patients' QoL and symptoms.
Financial Burden in Blood or Marrow Transplantation Survivors During the COVID-19 Pandemic: A BMTSS Report
Authors: Bhatia Smita , Dai Chen , Hageman Lindsey , Wu Jessica , Schlichting Elizabeth , Siler Arianna , Funk Erin , Hicks Jessica , Lim Shawn , Balas Nora , Bosworth Alysia , Te Hok Sreng , Francisco Liton , Bhatia Ravi , Forman Stephen J. , Wong F. Lennie , Arora Mukta , Armenian Saro H. , Weisdorf Daniel J. , Landier Wendy 
Published: December 2022

PURPOSE The financial burden experienced by blood or marrow transplant (BMT) survivors during the COVID-19 pandemic remains unstudied. We evaluated the risk for high out-of-pocket medical costs and associated financial burden experienced by BMT survivors and a sibling comparison group during the COVID-19 pandemic. METHODS This study included 2,370 BMT survivors and 750 siblings who completed the BMT Survivor Study survey during the pandemic. Participants reported employment status, out-of-pocket medical costs, and financial burden. Medical expenses ≥ 10% of the annual household income constituted high out-of-pocket medical costs. Logistic regression identified factors associated with high out-of-pocket medical costs and financial burden. RESULTS BMT survivors were more likely to incur high out-of-pocket medical costs (11.3% v 3.1%; adjusted odds ratio [aOR], 2.88; 95% CI, 1.84 to 4.50) than the siblings. Survivor characteristics associated with high out-of-pocket medical costs included younger age at study (aORper_year_younger_age, 1.02; 95% CI, 1.00 to 1.03), lower prepandemic annual household income and/or education (< $50,000 US dollars and/or < college graduate: aOR, 1.96; 95% CI, 1.42 to 2.69; reference: ≥ $50,000 in US dollars and ≥ college graduate), > 1 chronic health condition (aOR, 2.82; 95% CI, 2.00 to 3.98), ≥ 1 hospitalization during the pandemic (aOR, 2.11; 95% CI, 1.53 to 2.89), and being unemployed during the pandemic (aOR, 1.52; 95% CI, 1.06 to 2.17). Among BMT survivors, high out-of-pocket medical costs were significantly associated with problems in paying medical bills (aOR, 10.57; 95% CI, 7.39 to 15.11), deferring medical care (aOR, 4.93; 95% CI, 3.71 to 6.55), taking a smaller dose of medication than prescribed (aOR, 4.99; 95% CI, 3.23 to 7.70), and considering filing for bankruptcy (aOR, 3.80; 95% CI, 2.14 to 6.73). CONCLUSION BMT survivors report high out-of-pocket medical costs, which jeopardizes their health care and may affect health outcomes. Policies aimed at reducing financial burden in BMT survivors, such as expanding access to patient assistance programs, may mitigate the negative health consequences.
Best Practices and Guidelines (2022) for Scale-Up and Tech Transfer in Freeze-Drying Based on Case Studies. Part 1: Challenges during Scale Up and Transfer
Authors: Tchessalov Serguei , Shalaev Evgenyi , Bhatnagar Bakul , Nail Steven , Alexeenko Alina , Jameel Feroz , Srinivasan Jayasree , Dekner Michael , Sahni Ekneet , Schneid Stefan , Kazarin Petr , McGarvey Orla , Van Meervenne Bert , Kshirsagar Vaibhav , Pande Paritosh , Philipp Jens , Sacha Greg , Wu Ke , Azzarella Joseph , Shivkumar Gayathri , Bhatt Shreyas 
Published: December 2022

AbstractThe freeze-drying process scale-up and transfer remain a complicated and non-uniform practice. We summarized inefficient and good practices in these papers and provided some practical advice. It was demonstrated that using the same process set points/times in laboratory and commercial scale dryers may lead to loss of product quality (collapse or vial breakage). The emerging modeling approach demonstrated practical advantages. However, the upfront generation of some input parameters (vial heat transfer coefficient, minimum controllable pressure, and maximum sublimation rate) is essential for model utilization. While the primary drying step can be transferred with a high degree of confidence (e.g., using modeling), and secondary drying is usually fairly straightforward, predicting potential changes in product behavior during freezing remains challenging.
Graphical Abstract
Lysosomal alterations and decreased electrophysiological activity in CLN3 disease patient-derived cortical neurons
Authors: Chear Sueanne , Perry Sharn , Wilson Richard , Bindoff Aidan , Talbot Jana , Ware Tyson L. , Grubman Alexandra , Vickers James C. , Pébay Alice , Ruddle Jonathan B. , King Anna E. , Hewitt Alex W. , Cook Anthony L. 
Published: December 2022

ABSTRACT
CLN3 disease is a lysosomal storage disorder associated with fatal neurodegeneration that is caused by mutations in CLN3, with most affected individuals carrying at least one allele with a 966 bp deletion. Using CRISPR/Cas9, we corrected the 966 bp deletion mutation in human induced pluripotent stem cells (iPSCs) of a compound heterozygous patient (CLN3 Δ 966 bp and E295K). We differentiated these isogenic iPSCs, and iPSCs from an unrelated healthy control donor, to neurons and identified disease-related changes relating to protein synthesis, trafficking and degradation, and in neuronal activity, which were not apparent in CLN3-corrected or healthy control neurons. CLN3 neurons showed numerous membrane-bound vacuoles containing diverse storage material and hyperglycosylation of the lysosomal LAMP1 protein. Proteomic analysis showed increase in lysosomal-related proteins and many ribosomal subunit proteins in CLN3 neurons, accompanied by downregulation of proteins related to axon guidance and endocytosis. CLN3 neurons also had lower electrophysical activity as recorded using microelectrode arrays. These data implicate inter-related pathways in protein homeostasis and neurite arborization as contributing to CLN3 disease, and which could be potential targets for therapy.
Measuring the motives of informal entrepreneurs
Authors: Othman Noor Shahaliza , Marthandan Govindan , Ab Aziz Kamarulzaman 
Published: December 2022

Background - Handling non-observed activities pose major challenges to the governments and other stakeholders. Non-observed activities refer to underground activities, illegal activities, informal sector and any other activities that result in goods or services consumed by the household. The impact of these non-observed activities shows that the volume of people involved in the informal sector will rapidly increase. Informal economic activities are technically illegal yet are not intended as antisocial, thereby remaining acceptable to many individuals within the society. This research aimed to identify the factors that lead to entrepreneurial necessity and opportunity. Methods – The data of 51 respondents who were employed as informal entrepreneurs in Klang Valley areas in Malaysia was collected with the use of a questionnaire and convenient and proportionate sampling techniques. The data were analysed using SPSS software. Results – The two primary drivers of informal entrepreneurial activity were necessity and opportunity. The inability to find a formal job was an example of being driven by necessity. Meanwhile, individuals that are driven by opportunity chose to work independently in these informal sectors. Between necessity and engagement, refinement acted as a mediator. Often, necessity and opportunity do not automatically translate into successful entrepreneurship; further refinement is required in terms of market potential, technology usage, location preferences, and capital requirements. Improved refinement results in increased entrepreneurial engagement. Conclusions - The role and contribution of the informal sector entrepreneurship in economic development need to be evaluated and not just observed as an opportunity for individuals who choose this type of career. Therefore, further research is required in a wider variety of contexts to evaluate whether the same remains true in different populations. The results of this study can be useful for the government to set policies to encourage the transition of informal to formal entrepreneurships in Malaysia.
ANCA-associated vasculitis in Ireland: a multi-centre national cohort study
Authors: Scott Jennifer , Nic an Ríogh Eithne , Al Nokhatha Shamma , Cowhig Cliona , Verrelli Alyssa , Fitzgerald Ted , White Arthur , Walsh Cathal , Aslett Louis , DeFreitas Declan , Clarkson Michael R. , Holian John , Griffin Matthew D. , Conlon Niall , O’Meara Yvonne , Casserly Liam , Molloy Eamonn , Power Julie , Moran Sarah M. , Little Mark A. 
Published: December 2022

Background: Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a rare multisystem autoimmune disease. There is a need for interoperable national registries to enable reporting of real-world long-term outcomes and their predictors in AAV. Methods: The Irish National Rare Kidney Disease (RKD) registry was founded in 2012. To date, 842 patients with various forms of vasculitis have been recruited across eight nephrology, rheumatology and immunology centres. We focus here on patient- and disease- characteristics, treatment and outcomes of the 397 prospectively recruited patients with AAV. Results: Median age was 64 years (IQR 55–73), 57.9% were male, 58.9% had microscopic polyangiitis and 85.9% had renal impairment. Cumulative one- and five-year patient survival was 94% and 77% respectively. Median follow-up was 33.5 months (IQR 10.7–52.7). After controlling for age, baseline renal dysfunction (p = 0.04) and the burden of adverse events (p <0.001) were independent predictors of death overall. End-stage-kidney-disease (ESKD) occurred in 73 (18.4%) patients; one- and five-year renal survival was 85% and 79% respectively. Baseline severity of renal insufficiency (p = 0.02), urine soluble CD163 (usCD163) (p = 0.002) and “sclerotic” Berden histological class (p = 0.001) were key determinants of ESKD risk. Conclusions: Long-term outcomes of Irish AAV patients are comparable to other reported series. Our results emphasise the need for personalisation of immunosuppression, to limit treatment toxicity, particularly in those with advanced age and renal insufficiency. Baseline usCD163 is a potential biomarker for ESKD prediction and should be validated in a large independent cohort.
Cobertura y tratamiento informativo en periódicos de circulación nacional. Caso rifa del avión presidencial
Authors: Marañón Felipe , Saldierna Salas Alma Rosa , Moreno Marañón Dinorah 
Published: December 2022

El objetivo de este trabajo es determinar el tratamiento informativo que otorgan distintos periódicos de circulación nacional a la idea propuesta por el presidente de la República, Andrés Manuel López Obrador, sobre rifar el avión presidencial. A través de una metodología cuantitativa de análisis de contenido, y haciendo uso de encuadres o frames, se analizaron 84 notas periodísticas provenientes de cinco periódicos de circulación nacional. Los resultados arrojaron que se tuvo una gran presencia de atribución de responsabilidad como framing de acontecimiento narrado, siendo este el de mayor nivel en comparación con el discurso de interés humano, conflicto y consecuencias económicas. Así mismo se detectó el uso de un discurso de conspiración, en donde se posicionaba a la rifa como una estrategia para distraer a la población de otros asuntos políticos; sin embargo, también se encontraron una gran cantidad de notas en las que se brindó un discurso de apoyo. Remarcando de esta manera la importancia que tiene el periodismo en la generación de opiniones, así como en la de transmitir conocimiento a la población.
Institutional Investor Attention, Agency Conflicts, and the Cost of Debt
Authors: El Ghoul Sadok , Guedhami Omrane , Mansi Sattar A. , Yoon Hyo Jin 
Published: December 2022

Using a new measure of shareholder inattention constructed from exogenous industry shocks to institutional investor portfolios, we find that firms with distracted shareholders are associated with a higher cost of debt. This effect is stronger for firms with more powerful CEOs, firms with higher information asymmetry, and those operating in less competitive product markets. Further testing suggests that the inattention-cost of debt relation is driven primarily by dual holders directly observing shareholder distraction. Our results are robust to controlling for inattention at the retail investor level and to other external monitors, including credit rating agencies, financial analysts, and Big 4 auditors. Overall, our evidence suggests that institutional shareholder inattention has an incrementally negative effect on bond pricing. This paper was accepted by Brian Bushee, accounting. Supplemental Material: The data files and online appendix are available at https://doi.org/10.1287/mnsc.2022.4593 .
SUSTAINABILITY AND THE REASONS FOR ITS ADOPTION IN THE COMPANIES
Authors: Zhivkova Stela 
Published: December 2022

Sustainable development is all around us nowadays. Every day we are poured in with initiatives and information related to sustainable development. The world leaders talk about the green economy, circular economy, green deals, and sustainable development. However, what actually drives the companies to apply sustainable development principles in their business operations? Whether these are the different legislative requirements at the national and international level or the pure economic interest of the companies to earn additional income from their sustainable actions? The present paper highlights the key results of the project "International Business and Sustainable Practices" and attempts to summarize the motivations for the business to be sustainable. The research focuses on the three key dimensions of sustainability (economic, environmental, and social) and the sustainable development practices of internationally operating companies. The main objectives of the study are to identify the major business environment factors influencing the implementation of sustainable practices by companies operating internationally and to analyze the best sustainable development practices. The present study is primarily desk research based on the review of existing literature for developing and promoting the concept of sustainability in everyday business practices. Research papers, projects, and reports of international organisations working for sustainable development are extensively reviewed. The study found that internal environment factors like managers’ attitude, employees, and financial benefits are the key motives for the organisations to apply sustainable development practices in their business operations.