bluuebunny/crossref_metadata_2025_split huggingface dataset:
15-Lipoxygenase drives inflammation resolution in lymphedema by controlling PPARg+Treg cell population trafficking
Authors: Zamora Audrey , Benuzzi Emeline , Pujol Francoise , Morfoisse Florian , Baillif Vincent , Lacazette Eric , Galitzky Jean , Bouloumié Anne , Dubourdeau Marc , Chaput benoit , Fazilleau Nicolas , malloizel-delaunay Julie , Bura-Rivière Alessandra , Prats Anne-Catherine , Garmy-Susini Barbara 
Published: October 2022

Abstract
Lymphedema (LD) is characterized by the accumulation of protein-rich interstitial fluid, lipids and a significant inflammatory cell infiltrate in the limb. It causes a significant morbidity and is a common disabling disease affecting more than 150 million people worldwide, however there is no yet curative treatment. Here, we found that LD tissues from patients exhibit inflamed gene expression profile compared to their normal arm. Lipidomic analysis revealed severe decrease in arachidonic acid-derived lipid mediators generated by the 15-lipoxygenase (15-LO) in lymphedematous arms. Using a mouse model of lymphedema, we reproduced the etiology of the human pathology including the loss of specialized pro-resolving lipid mediators that play essential role in resolution of inflammation. This was associated with a lack of nonlymphoid PPARg-positive regulatory T cells (Treg) recruitment in the injured limb adipose tissue. Importantly, we identified the lymphatic endothelial 15-LO as responsible for the chemoattraction and survival of this Treg subpopulation. These results were confirmed by an aggravation of LD and degradation of the lymphatic network in an original transgenic mouse model in which ALOX15 gene has been selectively deleted in the lymphatic system (ALOX15lecKO). Importantly, this phenotype was rescued by the injection of ALOX15-expressing lentivectors. These results provide evidence that lymphatic 15-LO may represent a novel therapeutic target for LD by serving as a mediator of nonlymphoid Treg cell population invasion into lymphedematous adipose tissue to resolve inflammation.
Disentangling the seasonality effects of malaria transmission in the Brazilian Amazon Basin
Authors: Valiati Naiara , Villela Daniel 
Published: October 2022

Abstract

The current evidence of seasonality effects in the malaria epidemiology of the Brazilian Amazon Basin indicates the need for a rigorous examination in such a large geographical area. Furthermore, as seasonal effects are related to climate variables, malaria models should incorporate these effects.
This study applies wavelet analysis over incidence data in the SIVEP-malaria surveillance database. A mathematical model with climate-dependent parameterization is proposed to study counts of malaria cases over time based on notification data, temperature, and rainfall.
The wavelet analysis reveals marked seasonality in states such as Amazonas and Pará, whereas not so clear in recent years for states such as Acre and Pará. The wavelet cross-power analysis indicates a strong association between incidence and temperature, especially for cities Macapá and Manaus, and a similar association for rainfall. The mathematical model replicates temporal trends observed in incidences of these two cities.
Studies on climate-dependent mathematical models provide a good assessment of the baseline epidemiology of malaria. In addition, the knowledge of seasonality effects and the application of models have great potential as tools for studying interventions for malaria control.
Explaining Sexual Self-Care Status and its Predictor factors in Women Referring to Healthcare Centers of Sari, Iran, 2021
Authors: yazdani fereshteh , Simbar Masoumeh , HamzehGardeshi Zeinab , Zare Elham , Nasiri Malihe 
Published: October 2022

Abstract
Introduction

With the increase in the prevalence of non-communicable diseases and chronic health problems along with population growth, one of the definitions that is expanding is the ability to adapt and self-manage against diseases and self-care. Although there are several studies to examine self-care in medical conditions, there are not enough concepts and data related to sexual self-care. Therefore, the present study was conducted with the aim of explaining the status of sexual self-care and its predictor factors in women of reproductive age referring to healthcare centers.

Material and methods

This research was a cross-sectional study that was conducted on 400 women of reproductive age that referring to healthcare centers affiliated to Mazandaran University of Medical Sciences, Sari in 2021. Data were collected using personal-obstetric characteristics and sexual self-care questionnaires in women of reproductive age. Pearson correlation coefficients, independent t-test, one-way analysis of variance (ANOVA) and multiple linear regression model were used to analyze the data.

Results

The average total score of sexual self-care in reproductive age women participating in the research was 70.66% ± 12.52%. In addition, the prevention of women's cancers and the prevention of unintended pregnancies obtained the lowest and highest scores, respectively. Factors such as age, education, education related to medical sciences, history of HIV testing, history of HPV vaccination, source of sexual information, method of contraception and infection-related action in a person can affect the level of sexual self-care in women. Based on the final regression model, education related to medical sciences (B = 5.46, P = 0.035), family income (B = 5.58, P = 0.025), prevention method (B = 10.127, P = 0.000) and action related to infection in the person (B = 12.27, P = 0.047) were the final predictors of sexual self-care score in reproductive age women.

Conclusion

Based on the results of this study, education related to medical sciences, family income, contraceptive method and action related to infection are the final predictors of sexual self-care score in reproductive age women. The results of this study can be used to formulate interventions, and can be available to experts and policy makers to design programs to promote sexual self-care in women.
Unusual Manifestation of Gastric Adenocarcinoma presenting with Lymphedema, Chylothorax, and Chylous ascites: A Case Report
Authors: Wongrukmit Siree , Ngamphaiboon Nuttapong , Kiranantawat Kidakorn , Phanachet Pariya , Warodomwichit Daruneewan , Shantavasinkul Prapimporn Chattranukulchai 
Published: October 2022

Abstract
Background: Gastric cancer is one of most common cancers worldwide with a high mortality rate. Lymphedema and chyle leakage are unusual presentations of metastatic gastric carcinoma. We present a rare case of advanced stage gastric carcinoma presenting with lower limb lymphedema, bilateral chylothorax, and chylous ascites caused by metastatic infiltration of the lymphatics.
Case presentation: A 62-year-old Thai man with a 2-year history of bilateral lymphedema and an unprovoked left axillary vein thrombosis presented with progressive leg, scrotal, and abdominal swelling, and shortness of breath. He denied any gastrointestinal (GI) symptoms. His lymphedema had initially been diagnosed as chronic filariasis due to positive blood tests for anti-filarial antibodies, however treatment with anti-filarial drugs failed to improve his symptoms. Subsequently, he underwent surgical lymphaticovenular anastomosis with scrotal reduction, which proved to be of limited symptomatic relief. Later investigations revealed bilateral chylothorax and chylous ascites, with the presence of metastatic adenocarcinoma. Histopathological examination of the patient’s skin and scrotum biopsy from his previous surgery revealed invasion of the lymphatics by neoplastic cells with signet ring cell formation. Gastroscopy uncovered a gastric mass, and biopsy confirmed the diagnosis of stage IV gastric adenocarcinoma with signet ring cell. He later received palliative chemotherapy. For the management of chyle leakage, he was prescribed a very low-fat diet and supplemented with parenteral nutrition. Despite treatment, he developed cutaneous metastasis and was transitioned to best supportive care. The patient passed away 14 months after diagnosis.
Conclusion: The differential diagnosis of uncertain causes of lymphedema and chyle leakage should include metastatic gastric cancer, regardless of GI symptoms. Early upper GI endoscopy is a crucial diagnostic tool, as imaging alone may be insufficient to detect the primary lesion. Nutritional interventions form an integral component of chyle leakage management as it may help to reduce chyle flow, minimise nutrient loss, and improve patients’ nutritional status, leading to better survival and quality of life.
Dietary insulinemic potential, sleep quality and quantity in Iranian adults: Yazd Health Study and TAMYZ study
Authors: Sarsangi Peyman , Mohammadi Mohammad , Salehi-Abargouei Amin , Esmaillzadeh Ahmad , Mirzaei Masoud 
Published: October 2022

Abstract
Background & Aims:
To examine the link between dietary insulin index (DII) and load (DIL) and sleep duration/quality for the first time.
Methods
This cross-sectional study conducted on data from the recruitment phase of YaHS-TAMYZ prospective study in Yazd, central Iran. Data on demographic characteristics, dietary intakes, sleep quantity and quality, and potential confounders were gathered by interview. Sleep quality and its components (insufficient sleep, delay in falling asleep, medication use for sleep, and sleep disorder) were assessed by a modified version of Pittsburgh questionnaire. The link between DII/DIL and low sleep quality and short/long sleep duration was studied using multivariable logistic regression.
Results
In total, 5925 individuals aged 20 to 70 were eligible to take part in the current study. After adjustment for all potential confounders, participants in the highest DIL score tertile had a lower chance for sleep disorder (OR = 0.38; 95%CI: 0.17–0.85, Ptrend=0.02) and delay in falling asleep (OR = 0.66; 95%CI: 0.42–1.03, Ptrend=0.05) compared to those in the lowest tertile. The DII was also linked to a lower chance for sleep disorder (OR = 0.61; 95% CI: 0.39–0.93, P trend = 0.02). The DIL was inversely associated with sleep medication use and delay in falling sleep in men and women, respectively (P < 0.05). Moreover, DII was linked to a decreased odds of sleep disorder in women (P < 0.05). The associations were observed in those with overweight or obesity but not in those without overweight (P < 0.05).
Conclusion
Higher DIL and DII might be associated with sleep quality and its components. Prospective investigations are needed in the future to confirm these findings.
Mentoring &amp; Support Practices for Final Year Medical Students during a pandemic – ‘The Covid Doctors’
Authors: Brar Gurjot , Harney Sarah , McGarr Oliver , McFarland John 
Published: October 2022

Abstract
Background
Transition from final-year medical student to newly graduated doctor is challenging with evidence of associated increased patient mortality and medical errors. Previous work suggests tackling preparedness alone does not ‘solve’ this transition. The current focus on mentoring and support provision during this period and is an under-researched area. The COVID-19 pandemic represents a unique disruptive critical incident in which to examine mentoring and support practices, exposing strengths and weaknesses. The perspectives of this cohort and their implications remains an under-researched area.
Methods
Individual semi-structured interviews were conducted with nine graduate-entry final-year medical students. An inductive latent phenomenological approach explored individual experiences of mentoring and support practices during final-year and transition to professional practice.
Results
Three major themes emerged: 1) Mentoring & Support; 2) Clinical Exposure; 3) Graduation & Transition. A journey metaphor was used to aid the description of participants’ lived experience of mentoring and support practices during their final year. Final year medical students (FYMs) felt under-supported and found practices inadequate. Reduced clinical exposure yielded unpreparedness and regression, potentially impacting future careers. Positive experiences were variable and unstructured. ‘The COVID Doctors’, subtheme provided rich insights into shared narratives and identities amongst participants.
Discussion and Conclusion
Cultural change and reframing transitions as critically intensive learning periods may allow better engagement with the idiosyncrasies of workplace environments and support legitimate peripheral participation. Recommendations from the current study include updating contingency plans, balancing clinical exposure with patient safety issues, and providing support to ‘bottom-up’ mentoring practices.
Chaos of the coupled generalized Klein-Gordon equations with superlinear boundary conditions
Authors: Zhu Pengxian , Yang Jiaopeng 
Published: October 2022

Abstract
The complicated dynamics is considered for a system of coupled generalized Klein-Gordon equations with two symmetric superlinear boundary conditions. The coupled equations studied here becomes coupled damped Klein-Gordon equations or telegraph equations or wave equations at a certain parameter value. The system is reduced to a discrete iteration of a 2D map differing from the unary map induced by the non-coupled hyperbolic PDEs. In this article, the approach of snapback repeller is applied to establish the sufficient condition on the Li-Yorke chaos of the system. Moreover, by characterizing the asymptotic behavior of the 2D map, the global stability of the system is obtained under a certain range of parameter. In particular, the obtained dynamics not only extends the chaotic results on coupled wave equations with van der Pol type boundary conditions, but also gives the criterion of global stability.
Synergistic anticancer activity of cisplatin combined with tannic acid enhances apoptosis in lung cancer through the PERK-ATF4 pathway
Authors: Zheng Xiang , Yang Lei , Zhai Wei , Geng Nana , Zhang Zhimin , Wu Mingsong , Li Xueying 
Published: October 2022

Abstract
Background: Cisplatin (CDDP) is a common anticancer drug whose side effects limit its clinical applications. Tannins (TA) are plant-derived polyphenols that inhibit tumor
growth in different types of cancer. Here, we evaluated the anticancer effect of TA combined with CDDP on lung cancer cell lines (GLC-82 and H1299) and investigated the underlying molecular mechanism of endoplasmic reticulum (ER) stress-induced apoptosis.
Methods: Cell lines were treated with CDDP, TA, and CDDP+TA, and the effect of the combination was assessed using MTT assay and observed under light and fluorescence microscopes. Cell apoptosis was detected by flow cytometry, and the expression of key factors in the ER stress apoptotic pathway was detected using qRT-PCR and western blotting. The effects of the drug combination on the tumors of nude mice injected with H1299 cells were investigated, and the expression of key factors in the ER stress apoptotic pathway was investigated.
Results: The combination of CDDP and TA significantly inhibited lung cancer cell viability indicating a synergistic antitumoral effect. The mRNA and protein expression levels of key ER stress factors in the CDDP+TA group were considerably higher than those in the CDDP and TA groups, the tumor volume in tumor-bearing mice was the smallest and the number of apoptotic cells and the protein expression levels of the key ER stress in the combination group were considerably higher.
Conclusions: The combination of TA and CDDP may produce synergistic antitumoral effects, mediated by the PERK-ATF4-CHOP apoptotic axis, suggesting a novel adjuvant treatment for lung cancer.
Putative adverse outcome pathway for silver nanoparticle toxicity on male reproductive system: A literature review
Authors: Kose Ozge , Mantecca Paride , Costa Anna , Carrière Marie 
Published: October 2022

Abstract
Background: Adverse outcome pathways (AOPs) are conceptual frameworks that organize knowledge about biological interactions and toxicity mechanisms. They present a sequence of events commencing with initial interaction(s) of a stressor, which defines the perturbation in a biological system (molecular initiating event, MIE), and a dependent series of key events (KEs), ending with an adverse outcome (AO). AOPs have recently become the subject of intense studies in a view to better understand the mechanisms of nanomaterial (NM) toxicity. Silver nanoparticles (Ag NPs) are one of the most explored nanostructures and are extensively used in various application. This, in turn, has increased the potential for interactions of Ag NPs with environments, and toxicity to human health. The aim of this study was to construct a putative AOPs (pAOP) related to reproductive toxicity of Ag NPs, in order to lay the groundwork for a better comprehension of mechanism affecting both undesired toxicity (against human cell) and expected toxicity (against microorganisms).
Methods: PubMed and Scopus were systematically searched for peer-reviewed studies examining reproductive toxicity potential of Ag NPs. The quality of selected studies was assessed through ToxRtool. Eventually, forty-eightstudies published between 2005 and 2022 were selected to identify the mechanisms of Ag NPs impact on reproductive function in male. The biological endpoints, measurements, and results were extracted from these studies. Where possible, endpoints were assigned to a potential KE and an AO using expert judgment. Then, KEs were classified at each major level of biological organization.
Results: We identified the impairment of intracellular SH-containing biomolecules, which are major cellular antioxidants, as a putative MIE, with subsequent KEs defined as ROS accumulation, mitochondrial damage, DNA damage and lipid peroxidation, apoptosis, reduced production of reproductive hormones and reduced quality of sperm. These successive KEs may result in impaired male fertility (AO).
Conclusion: This research recapitulates and schematically represents complex literature data gathered from different biological levels and propose a pAOP related to the reproductive toxicity induced by AgNPs. The development of AOPs specific to NMs should be encouraged in order to provide new insights to gain a better understanding of NP toxicity.
Electronic structure, cationic and excited states of nitrogen-containing spiroborates
Authors: Sidorin Andrey , Tikhonov Sergey , Samoilov Ilya , Osmushko Ivan , Svistunova Irina , Tretyakova Galina , Puzyr'kov Zahar N. , Vovna Vitaliy 
Published: October 2022

Abstract
Electronic structure, cationic and excited states of three spiroborate complexes (2-acetylacetonato-1,3,2-benzodioxaborol, its NH- and NMe-derivatives) and three corresponding ligands (acetylacetone, 4-aminopent-3-en-2-one and 4-methylaminopent-3-en-2-one) were studied by photoelectron spectroscopy, absorption spectroscopy, high-level ab initio quantum chemical computations, including the algebraic diagrammatic construction method for the polarization propagator of the second order, the outer-valence Green's function method, the density functional approach, and the time-dependent density functional approach. Analysis of experimental and calculated data allowed determining the influence of functional groups on the parameters of the electronic structure and energy of electronic transitions. Compared to acetylacetone and its NH- and NMe-derivatives, the upper filled molecular orbitals of the corresponding spiroborates are stabilized at 0.4–1.7 eV, which is due to the positive charge of the ligand due to the acceptor properties of the dioxyphenylene fragment. Among the studied compounds, when replacing the oxygen atom in the α-position with the NH- or NMe-group, a bathochromic shift of intense bands in the absorption spectra is observed, since the energy intervals between the orbitals of the π3 and π4 ligand are reduced. In addition, in a number of spiroborates, the violation of C2v symmetry when replacing an oxygen atom leads to the appearance of a low-intensity maximum in the long-wave part of the absorption spectrum, due to the π2X → π4 transition.
Influence of masticatory side switch frequency on chewing mixing ability and sensory perception in adults with healthy dentitions: A randomized crossover trial
Authors: Ignatova-Mishutina Tatiana , Khoury-Ribas Laura , Flores-Orozco Elan Ignacio , Rovira-Lastra Bernat , Martinez-Gomis Jordi 
Published: October 2022

Abstract
Objectives

This study aimed to determine the effect of varying the frequency of masticatory side switches on chewing mixing ability and sensory perception in dentate adults.

Materials and Methods

This non-blinded, randomized, twelve-period crossover study, conducted at Barcelona Dental school from January to March 2022, included 36 healthy adults with natural dentitions (median age, 23.5 years; 26 women). We randomly allocated participants to 12 sequences and performed 12 masticatory assays chewing a two-colored gum for 40 cycles each using the following masticatory styles as interventions: freestyle, unilateral right, unilateral left, and switching sides 5%, 15%, and 25%. The primary outcome was the Mixing Ability Index (MAI), defined as the standard deviation of the red channel intensity of the chewed gum in the Color-Histogram plugin of ImageJ. Participants also rated the perceived flavor intensity and salivary flow on a visual analog scale. Data were analyzed by repeated measures analysis of variance.

Results

The MAI was similar for all masticatory styles (P = 0.63). Participants perceived greater flavor intensity (mean difference, 8%; 95%CI, 1–15%) and salivary flow (mean difference, 11%; 95%CI, 0–21%) with 25% side switching compared to freestyle or unilateral chewing.

Conclusions

Frequently switching the masticatory side while chewing gum does not alter the mixing ability, but it does seem to enhance salivary flow and flavor intensity.

Clinical relevance

In dentate adults, increasing the frequency of masticatory side switching to 25% does not impair mixing, but it may improve salivary production and flavor intensity.

Trial registration

ClinicalTrials.gov Identifier: NCT05173259
Altered DNA repair related proteins in Parkinson’s disease model VMAT2 Lo mice
Authors: Parker Karsten , Zeng Fei , Zhan Yanqiang , Miller Matthew , Zhu Meng-Yang 
Published: October 2022

Abstract
DNA damage and repair processes play an important role in the pathogenesis of age-related neurodegenerative diseases such as Parkinson’s Disease (PD), as DNA repair pathways delay cell senescence and aging by maintaining genomic integrity. In the present study, the expression of DNA repair-related enzymes and proteins were examined in the brain of VMAT2 Lo mice, a PD animal model. The results demonstrated that in the frontal cortex (FC) and locus coeruleus (LC) of VMAT2 Lo mice at 2, 6, and 15 months of age, OGG1 protein levels were significantly increased. However, OGG1 protein levels in the hippocampus, substantia nigra (SN) and LC of these model mice at 18 and 23 months of age exhibited a marked reduction. This reduction of OGG1 proteins in the hippocampus and SN was accompanied by the relatively similar diminishment of their mRNAs. Furthermore, immunochemical and immunofluorescence staining demonstrated that in most measured brain regions, the immunoreactivities of PARP1, ERCC1, XRCC1 and PCNA, four enzymes and protein related to DNA repair processes, were considerably reduced in VMAT2 Lo mice at 18 and 23 months of age. These analysis results reveal the DNA oxidative damage triggers the activation of DNA repair process in this PD model, and provides important insights for the involvement of DNA repair processes in the PD pathogenesis.