bluuebunny/crossref_metadata_2025_split huggingface dataset:
Limitations and safety concerns related to the currently used cervical orthoses in preclinical emergency settings
Authors: Gherlea Dorin , Garyuk Grygoriy , Ianăş Florin , Blattert Thomas R. 
Published: October 2022

AbstractResearch question:Are the currently used cervical orthoses suitable for immobilizing the cervical spine for patients with preexisting ankylosing spondylitis, an anatomically short neck, or obesity?Materials and Methods:Tools, immobilization methods, completion time, sagittal displacement, and feedback on result were compared for one control and two severe case scenarios simulated on appropriately built manikins. Scenario 1 simulated a young patient who sustained a bicycle fall with no fracture. Scenario 2 simulated a short-necked middle-aged patient who met a vehicle accident and showed C7/T1 bilateral facet dislocation fracture. Scenario 3 simulated an elderly patient with preexisting ankylosing spondylitis who fell down stairs and sustained a C6/C7 non-displaced fracture.Results:For scenarios 1 and 2, the immobilization rate with the collar was 100% vs. 86%, average completion time was 90 s vs. 175 s, rate of attempts involving tool changes was 16% vs. 49%, rate of attempts with the collar forced in position was 0% vs. 32%, and proportion of cases with satisfactory results was 100% vs. 5%. For scenarios 1 and 3, the immobilization rate with the collar was 100% vs. 16%. For scenario 3, the rates of critical and highly critical sagittal deviation were 54% and 13.5%, respectively, while the efficiency of immobilization was 0%.Discussion and conclusion:Currently used rigid collars can increase the time on-scene and tamper with spinal alignment. They cannot be easily and safely applied to elderly, overweight patients and those with an anatomically short neck and can diminish the outcomes for these patients.
In-Situ Quality Assessment and Control in Fused Filament Fabrication (FFF) Using Laser Surface Profilometer and Deep Learning (DL) Techniques
Authors: Akhavan Javid , Lyu Jiaqi , Manoochehri Souran 
Published: October 2022

Abstract
Additive Manufacturing (AM) has become one of the most popular manufacturing techniques in various fields. Their layer-by-layer printing process allows easier fabrication of complex geometries. However, the quality and accuracy of fabricated artifacts in these techniques have low repeatability. In the era of Industry 4.0 by using emerging sensory and data processing capabilities such as Laser Surface Profilometer (LSP) and Deep Learning (DL), it is possible to improve the repeatability and quality of AM processes. This work presents an in-situ quality assessment and improvement using LSP for data acquisition and DL for data processing and decision making. The utilized LSP module generates a point cloud dataset containing information about the top surface geometry and quality. Once the point cloud data is pre-processed, an improved deep Hybrid Convolutional Auto-Encoder decoder (HCAE) model is used to perform the artifact's quality measurement and statistical representation. The HCAE model's statistical representation is comprised of 9*9 segments, each including four channels with the segment's probability to contain one of four labels, 1) Under-printed region, 2) Normally printed region, 3) Over-printed region, 4) Empty region. This data structure plays a significant role in determining the commands needed to optimize the fabrication process. The implemented HCAE model's accuracy and repeatability were measured by a multi-label multi-output metric developed in this study. The assessments made by HCAE are then used to perform an in-situ process adjustment by manipulating the future layer's fabrication through the G-code modification. By adjusting the machine's print speed and feedrate, the control algorithm exploits the subsequent layer deposition, segment by segment. The algorithm is then tested with two settings that can cause defective parts: severe under-extrusion and over-extrusion conditions. Both test artifacts' quality advanced significantly and converged to an acceptable state by four iterations.
A novel method for analyzing the contribution of risk factors for the severity of Xiyanping Injection’s adverse events, 2004-2020
Authors: Zheng Rui , Chen Zhao , Guan Zhiyue , Zhao Chen , Cui Herong , Shang Hongcai 
Published: October 2022

Abstract
Objectives: Based on previous studies, analyze risk factors (age and combination) contributing to the severity of Xiyanping Injection’s (XYP) adverse events (AEs) by a novel method.
Methods: Total AEs linked to the use of XYP injection were classified as general or serious and analyzed in the China National Adverse Drug Reaction Monitoring Information System (2004-2020). Data were analyzed with respect to age and herb-drug combination (ribavirin, ceftriaxone, penicillin sodium, ambroxol hydrochloride, clindamycin, cefoxitin sodium, azithromycin, ceftazidime, amoxicillin sodium and clavulanate potassium, levofloxacin hydrochloride, cefazolin sodium pentahydrate and cefuroxime) by variable importance for projection (VIP).
Results: 28599 AEs (general27037, severe 1562) relating to XYP’s combination were included. Based on the VIP value and correlation coefficient, the results showed that in 0-6 years old, XYP -ribavirin, -ceftazidime and -ambroxol hydrochloride combination indicate positive impact on the severity of AEs.
In 7-17 years old, XYP-cefoxitin sodium and -azithromycin combination tended to have general AEs. XYP-cefazolin sodium pentahydrate and -amoxicillin sodium and clavulanate potassium combination tended to have severe AEs. In 18-40 years old, XYP-cefoxitin sodium combination tended to have general AEs. Under the influence of XYP- ribavirin and -clindamycin combination, it tended to have severe AEs. In 41-65 years old, Under the influence of XYP-cefatriaxone, -penicillin sodium and -cefoxitin sodium combination, it tended to have severe AEs. In＞65 years old, XYP-RB, clindamycin and -amoxicillin sodium and clavulanate potassium combination tended to have general AEs. Under the influence of XYP-cefoxitin sodium and -ceftazidime combination, it tended to increase the severity of AEs. The two groups had the same impact on the severity of AEs.
Conclusions: VIP scores are useful in evaluating the risk factors affecting outcome indicators in clinical studies. It is recommended that clinical practitioners should pay attention to the metabolic characteristics of different age groups and strictly implement standardized operations such as medication interval and flushing.
Ocean Gravity Wave Split from the Meteotsunami at the Japan Trench Following the 2022 Tonga Volcanic Eruption
Authors: Ho Tung-Cheng , Mori Nobuhito , Yamada Masumi 
Published: October 2022

Abstract
The 2022 eruption of the Hunga Tonga-Hunga Ha'apai volcano excited an atmospheric Lamb wave, which induced a fast-traveling tsunami. This tsunami was driven by the pressure-forced wave traveling at the speed of the Lamb wave, and thus, was much faster than conventional tsunamis. This was the first case in which ocean bottom monitoring systems widely observed an air pressure-induced tsunami. The observation showed that the pressure-forced wave split after passing the Japan trench. Our simulations show that changes in water depth can amplify or decrease the pressure-forced wave. Simultaneously, an ocean gravity wave is generated due to the conservation of water volume. Because the ocean gravity wave was slower than the pressure-forced wave near Japan, it was separated from, and traveled behind, the pressure-forced wave. We reproduced the waveforms of different splitting stages observed by the stations near the Japan Trench.
Fine mapping of genes controlling pigments accumulation in oilseed rape (Brassica napus L.)
Authors: Chen Daozong , Jin Qingdong , Pan Jiangming , Liu Yi , Tang Yijia , E Yanrong , Xu Linshan , Yang Taihua , Qiu Jie , Chen Xiaodi , Wang Jing , Gong Deping , Ge Xianhong , Li Zaiyun , Cui Cheng 
Published: October 2022

Abstract
Purple/red appearance is one of the common phenotypic variations in leaves, stems, and siliques of oilseed rape (Brassica napusL.) but very rare in flowers. In this study, the causal genes for the purple/red traits in stems and flowers in two accessions of oilseed rape (DH_PR and DH_GC001, respectively) derived from the wide hybridization were fine mapped and candidate genes were determined by methods combined with bulked segregant analysis (BSA) and RNA-seq analysis. Both purple stem trait and red flowers trait were mapped to the locus as the AtPAP2 homologous genes (BnaPAP2.C6a and BnaPAP2.A7b, respectively) belonging to the R2R3-MYB family. Sequencing of the BnaPAP2.C6a gene in purple stem accession revealed several Indels and SNPs in its promoter region, intron 1 as well as exons. However, only a 211bp insertion was identified in promoter region of BnaPAP2.A7b of DH_GC001. Our results not only contribute to a better understanding of anthocyanin inheritance in B. napus, but also provide a useful toolbox for future breeding of cultivars with purple/red traits through combination of different functional alleles and homologs.
The impact of geographical and sociodemographic variation on access to Systemic Anti- Cancer Therapies for Secondary Breast Cancer: A systematic review
Authors: Pearson Sally Anne , Taylor Sally , Marsden Antonia , Dalton Jessica , Krishan Ashma , Yorke Janelle , Howell Sacha 
Published: October 2022

Abstract
Background:
The review aimed to investigate factors which influence receipt of systemic anti-cancer therapies (SACT) for women with secondary (metastatic) breast cancer (SBC). We aimed to identify and examine individual, clinical and contextual factors related to geographical location and health care systems which may act as barriers and enabling factors.
Methods:
Studies were included which reported factors associated with receipt of treatment with SACT for women >18 years with an SBC diagnosis. Information sources searched were EBSCO CINAHL Plus, Ovid MEDLINE, Ovid EMBASE, PsychINFO and the Cochrane Library and Joanna Briggs Institute (JBI) database. Assessment of methodological quality was undertaken using the using the JBI method and findings were synthesised using a narrative synthesis approach.
Results:
Fifteen studies published between 2009 and 2021 were included in the review. Overall treatment receipt ranged from 4% for immunotherapies to 83% for unspecified systemic anti-cancer therapies. Time to treatment ranged from median 54 days to 95 days with 81% of patients received treatment <60 days.
Younger women and women of white origin with a higher socioeconomic status had an increased likelihood of timely treatment receipt. Treatment receipt varied by geographical location and place of care was associated with variation in treatment receipt with women treated at teaching, research and private institutions being more likely to receive treatment in a timely manner.
Conclusions:
Our review was to our knowledge the first of its kind to identify and investigate factors associated with timely receipt of SACT for women with SBC. We identified a potential interaction between geographical location and place of care which adds to the existing literature. Findings should however be interpreted with a degree of caution due to the limitations identified. Further research is required to address these limitations.
Our review findings have practical implications for the development and piloting of targeted interventions to address specific barriers in a socio-culturally sensitive manner. Addressing geographical variation and place of care may require intervention at a commissioning policy level. Further qualitative research is required to understand the experience and of women and clinicians.
Other:
The review was undertaken as part of a PhD fellowship funded by The Christie Hospital NHS Foundation in collaboration with the Manchester Cancer Research Centre (MCRC) at The University of Manchester.
The review protocol was registered in PROSPERO CRD42020196490.
Effects of traditional Chinese medicine on allergic asthmatic mice: using a combined formula
Authors: Tsai Jaw-Ji , Yen Chung-Yang , Hsu Chun-Hsien , Yu Sheng-Jie , Chen Chao-Hsien , Liao En-Chih 
Published: October 2022

Abstract
Background
Allergic asthma occurs worldwide and is particularly prevalent in westernized countries characterized by chronic airway inflammation resulting in airway hyperresponsiveness. The house dust mites (HDM) including Dermatophagoides pteronyssinus are major sources of sensitization and triggering allergic symptoms in asthmatic patients. The Der p 2 is a major allergen and the predominant source of causative respiratory disorders which induce airway inflammation and bronchial constriction in mite-allergic patients. Few studies evaluate the ameliorating effects of modified Liu-Wei-Di-Huang-Wan (modified LWDHW) on allergic asthma.
Methods
This study aimed to investigate the immunological mechanisms of modified LWDHW on the inhibitory effects of airway inflammation, signal transduction, inflammatory cytokine production, Th2 cell proliferation, and bronchial obstruction in Der p 2-induced asthmatic mice.
Results
At least ten active ingredients were contained in the formula of modified LWDHW- 1217A and 1217B. Results showed that the immunoglobulin generations (Der p 2 specific- IgE and IgG1), inflammatory cytokine productions (IL-5 and IL-13) in the Sera and BALF could be down-regulated, and the Th1-cytokine productions (IL-12 and IFN-γ) be increased after immunotherapy with modified LWDHW of 1217A or 1217B. The inflammatory cell infiltrations (macrophages, eosinophils, and neutrophils) in the airway and the expressions of TH2-related genes (IL4, IL5, and IL13), TH2-related transcription factor (GATA-3), and neutrophil chemotactic chemokine (IL8) in the lung tissue of asthmatic mice were significantly decreased after the immunotherapy. The Th1/Th2 polarization had been identified that the IL-4+/CD4+ T cells were downregulated and IFN-γ+/CD4+ T cells were increased. The airway hyperresponsiveness to methacholine inhalation of Penh values was significantly decreased in the treated groups. There were significant improvements in the bronchus histopathology after immunotherapy with 1217A or 1217B which were evaluated by tracheal thickness, inflammatory cell count, and tracheal rupture of mouse lung.
Conclusions
It revealed that 1217A or 1217B could regulate the immune responses and improve pulmonary function. Data suggests that modified LWDHW of 1217A or 1217B have the potential for use as a therapeutic modality for the treatment of mite allergen Der p 2-induced allergic asthma.
Integrating animal experiment, mass spectrometry and network-based approach to reveal the improving sleep quality of Ziziphi Spinosae Semen and γ-aminobutyric acid mixture
Authors: Ren Airong , Wu Tingbiao , Wang Yarong , Fan Qing , Yang Zhenhao , Zhang Shixun , Cui Guozhen 
Published: October 2022

Abstract
Background
Ziziphi Spinosae Semen (ZSS), which is widely consumed as a medicine and food dual-use plant in some regions of Asian countries, has various health benefits. γ-aminobutyric acid (GABA), a non-proteinaceous amino acid, is one of the major neurotransmitter inhibitors that has a relaxant function. In this study, a system pharmacology approach was proposed to evaluate the effects of a mixture composed by ZSS and GABA (ZSSG) on sleep improvement.
Methods
Mice were divided into 5 groups (n = 10) and received either no treatment or sodium pentobarbital or sodium barbital with Diaz or ZSSG. The effects of ZSSG on improving sleep quality were evaluated in mice. The major ingredients of ZSSG were analyzed by UPLC-Q/TOF-MS. Network-based ingredient-insomnia proximity was applied to explore its major ingredients.
Results
The results showed that ZSSG displayed significant effects on decreasing sleep latency and prolonging sleep duration in sodium pentobarbital-induced sleeping mouse model (P < 0.05). ZSSG significantly enhanced the brain content of GABA in mice. In addition, ZSSG also significantly decreased sleep latency-induced by sodium barbital in mice (P < 0.05). With the network-based ingredient screening model, four active ingredients and three pairwise ingredient combinations were discovered from ZSSG among 85 ingredients in ZSSG identified by UPLC-Q/TOF-MS.
Conclusion
These data demonstrated that ZSSG improved the sleeping quality of mice. The potential four active ingredients and three pairwise ingredient combinations could serve as quality markers associated with insomnia. The network-based framework may open up a new avenue for the discovery of active ingredients of herbal medicine for treating complex chronic diseases, such as insomnia.
Maternal and perinatal outcomes in mixed antenatal care modality implementing telemedicine in the southwestern region of Colombia
Authors: Escobar María Fernanda , Gallego Juan Carlos , Echavarria María Paula , Fernandez Paula , Posada Leandro , Salazar Shirley , Gutierrez Isabella , Alarcón Juliana 
Published: October 2022

Abstract

Introduction: Contingency measures due to the COVID-19 pandemic limited access to routine prenatal care for pregnant women, increasing the risk of pregnancy complications due to poor prenatal follow-up, especially in those patients at high obstetric risk. This prompted the implementation and adaptation of telemedicine.
Objective: We aim to evaluate the maternal and perinatal outcomes of patients who received prenatal care in-person and by telemedicine.
Methods: We conducted a retrospective observational cohort study of pregnant women who received exclusive in-person and alternate (telemedicine and in-person) care from March to December 20202, determining each group's maternal and neonatal outcomes.
Results: A total of 1078 patients were included, 156 in the mixed group and 922 in the in-person group. The patients in the mixed group had a higher number of prenatal controls (8 (6-9) vs 6 (4-8) p<0.001), with an earlier gestational age at onset (7.1 (6-8.5) vs 9.3 (6.6-20.3), p<0.001), however, they required a longer hospital stay (26 (16,67%) vs 86 (9,33%), p=0.002) compared to those attended in-person; there were no significant differences in the development of obstetric emergencies, maternal death or neonatal complications.
Discussion: Incorporating telemedicine mixed with in-person care could be considered as an alternative for antenatal follow-up of pregnant women in low- and middle-income countries with barriers to timely and quality health care access.
Modular DNA-origami-based nanoarrays enhanced cell binding-affinity through “lock-key” interaction
Authors: Mao Miao , Lin Zhun , Chen Liang , Zou Zhengyu , Zhang Jie , Dou Quanhao , Wu Jiacheng , Chen Jinglin , Wu Minhao , Niu Li , Zhang Yuanqing 
Published: October 2022

Abstract
Surface proteins of cells are generally recognized through receptor-ligand interactions (RLIs) in disease diagnosis, but their nonuniform spatial distribution and high-order structure lead to low binding-affinity. Constructing nano-topologies that match the spatial distribution of membrane proteins to improve binding-affinity remains a challenge. Inspired by the multi-antigen recognition of immune synapses, we developed modular DNA-origami-based nanoarrays with multivalent aptamers. By adjusting the valence and inter-spacing of the aptamers, we construct specific nano-topology to match the spatial distribution of target protein clusters and avoid potential steric hindrance. We found that the nanoarrays significantly enhanced the binding-affinity of target cells, and synergistically recognize low-affinity antigen-specific cells. In addition, using DNA nanoarrays for the detection of clinical circulation tumor cells successfully verified its precise recognition ability and high affinity RLIs. Such nanoarrays will further promote the potential application of DNA materials in clinical detection and even cell membrane engineering.
Structure of a G protein-coupled receptor with GRK2 and a biased ligand
Authors: Xu H. Eric , Duan Jia , Liu Heng , Ji Yujie , Yuan Qingning , Li Xinzhu , Wu Kai , Gao Tianyu , Zhu Shengnan , Jiang Yi , Yin Wanchao 
Published: October 2022

Abstract
Phosphorylation of G protein-coupled receptors (GPCR) by GPCR kinases (GRKs) desensitizes G protein signaling and promotes arrestin signaling, which is also modulated by biased ligands. Molecular assembly of GRKs to GPCRs and the basis of GRK-mediated biased signaling remain largely unknown due to the weak GPCR-GRK interactions. Here we report the complex structure of neurotensin receptor 1 (NTSR1) bound to GRK2, Gαq, and an arrestin-biased ligand, SBI-553, at a resolution of 2.92 Å. The high-quality density map reveals the clear arrangement of the intact GRK2 with the receptor, with the N-terminal helix of GRK2 docking into the open cytoplasmic pocket formed by the outward movement of the receptor TM6, analogous of the binding of G protein to the receptor. Strikingly, the arrestin-biased ligand is found at the interface between GRK2 and NTSR1 to enhance GRK2 binding. The binding mode of the biased ligand is compatible with arrestin binding but is clashed with the binding of a G protein, thus provide an unambiguous mechanism for its arrestin-biased signaling capability. Together, our structure provides a solid model for understanding the details of GPCR-GRK interactions and biased signaling.
Design of a new unsymmetrical bis(imino)pyridine Schiff-base complexes with ionic liquid group immobilized on nano-magnetite particles in catalyzing green reactions of Chromenes as a recyclable heterogeneous catalyst: optimization using Taguchi methodology
Authors: Mohammadian Reza , Sandaroos Reza , Allahresani Ali 
Published: October 2022

AbstractA new unsymmetrical Cobalt complex containing ionic liquids (ILs) group immobilized on Fe3O4@SiO2was prepared and characterized by H-NMR, FTIR, SEM, EDX, TGA, XRD, and VSM analyses. The experimental design was performed using the Taguchi method to reduce costs and time. We examined four levels of solvents, solvent amounts, temperatures, and catalyst amounts. Software Minitab was used to evaluate each parameter's impact on the sample reaction of Chromene. Using data analysis, it was determined that solvent, solvent amount, temperature, and catalyst amount contributed 33.20, 15.00, 22.33, and 28.12%, respectively, to the efficiency of the reaction. The derivatives of Chromenes were synthesized at achieved optimum conditions. The presence of ionic liquid groups increased the solubility of the heterogeneous catalyst in green solvents, which increased the efficiency and reduced the reaction time.