bluuebunny/crossref_metadata_2025_split huggingface dataset:
Socioeconomic factors influencing knowledge and consumption of food plants by a human group in a mountainous environment in the semiarid region of Bahia, Northeast Brazil
Authors: da Silva Souza Luciana Vitor , Marques Juracy , de Oliveira Campos Letícia Zenóbia , de Freitas Lins Neto Ernani Machado 
Published: June 2022

Abstract
Background
The relationship of people with natural resources is guided by different sociocultural, ecological and evolutionary factors. Regarding food plants, it is not different. Studies around the world have evaluated the effects of socioeconomic factors, such as age, gender, income, profession, education level, time of residence, ethnic diversity, religion, festive rituals, access to urban areas and migrations. In this sense, the objective of the present study was to characterize the diversity of knowledge and use of food plants by people from Serra dos Morgados and evaluate if the socioeconomic factors influence knowledge and consumption of food plants in the community.

Methodology
This research was conducted in the village of Serra dos Morgados, municipality of Jaguarari, Bahia, with the purpose of evaluating the factors that influence in the knowledge and use of food plants. Socioeconomic data such as age, gender, time of residence, and monthly income were collected. The free list technique was applied during the collection of ethnobotanical data in order to analyze the preference of the plants based on the salience index (SI). To analyze the factors that influence knowledge and use forms, we used GLM Lasso.

Results
A total of 33 people were interviewed, 8 men and 25 women; their age ranged from 30 to 82 years. People cited 98 species of plants, 41 species being identified of spontaneous occurrence. The plant with the highest salience index (SI) was “cheirosa” (Psidium ganevii) (SI = 0.5679), followed by “massaranduba” (Micropholis sp.) (SI = 0.4323); “araça” (Campomanesia guazumifolia) (SI = 0.3320); and “cambuí” (Siphoneugena sp.) (SI = 0.3144).

Conclusions
The main factors that influence knowledge and use forms in the locality were family income and the collection site, with homegardens cited as the preferred area for collection of food plants. This study provided an overview related to potentially important species for a community located in a region where there are few ethnobiological studies. The results presented here can be used in future studies, providing clues for investigations. Also, there is a contribution to the conservation of biocultural aspects related to the use of food plants in a community living in mountainous regions.
The E6 and E7 proteins of beta3 human papillomavirus 49 can deregulate both cellular and extracellular vesicles-carried microRNAs
Authors: Chiantore Maria Vincenza , Iuliano Marco , Mongiovì Roberta Maria , Dutta Sankhadeep , Tommasino Massimo , Di Bonito Paola , Accardi Luisa , Mangino Giorgio , Romeo Giovanna 
Published: June 2022

Abstract
Background
The β3 human papillomavirus (HPV)49 induces immortalization of primary keratinocytes through the action of E6 and E7 oncoproteins with an efficiency similar to alpha high risk (HR)-HPV16. Since HR-HPV oncoproteins are known to alter microRNA (miRNA) expression and extracellular vesicle (EV) production, we investigated the impact of HPV49 E6 and E7 proteins on miRNA profile and EV expression, and their involvement in the control of cell proliferation.

Methods
The miRNA expression was evaluated by a miRNA array and validated by RT-qPCR in primary human keratinocytes immortalized by β3 HPV49 (K49) or α9 HR-HPV16 (K16), and in EVs from K49 and K16. The modulation of miRNA target proteins was investigated by immunoblotting analyses.

Results
By comparing miRNA expression in K49 and K16 and the derived EVs, six miRNAs involved in HPV tumorigenesis were selected and validated. MiR-19a and -99a were found to be upregulated and miR-34a downregulated in both cell lines; miR-17 and -590-5p were upregulated in K49 and downmodulated in K16; miR-21 was downregulated only in K16. As for EV-carried miRNAs, the expression of miR-17, -19a, -21 and -99a was decreased and miR-34a was increased in K49 EVs. In K16 EVs, we revealed the same modulation of miR-19a, -34a, and -99a observed in producing cells, while miR-21 was upregulated. Cyclin D1, a common target of the selected miRNAs, was downmodulated in both cell lines, whereas cyclin-dependent kinase 4 was down-modulated in K49 but upregulated in K16.

Conclusion
These data suggest that E6 and E7 proteins of β3 HPV49 and α9 HR-HPV16 affect key factors of cell cycle control by indirect mechanisms based on miRNA modulation.
Crisis upon crisis: a qualitative study exploring the joint effect of the political, economic, and pandemic challenges in Lebanon on Syrian refugee women’s fertility preferences and behaviour
Authors: Mourtada Rima , Melnikas Andrea J. 
Published: June 2022

Abstract
Background
Starting in October 2019, Lebanon experienced overlapping crises that caused a significant deterioration of the living conditions for Syrian refugees and the host community. Previous studies have shown that difficult living conditions and refugee status alone do not impact the fertility preferences of Syrian refugees. This study seeks to explore the effect of the overlapping crises on the fertility preferences and behaviour of Syrian refugees in Lebanon.

Methods
In this qualitative study, we carried out focus group discussions (FGDs) with married female Syrian refugees recruited purposively from two cities in West Bekaa (Bar Elias and Saad Nayel) and from inside and outside the Informal Tented Settlements (ITS). Transcripts were analysed using thematic analysis.

Results
The overlapping crises (political, economic, and Covid-19) in Lebanon influence Syrian refugee women’s reported desire for fewer children. Two themes emerged that explained the change in Syrian refugees’ fertility preferences towards limiting their number of children or delaying having children, and potentially a change in their fertility practices: the sudden deterioration in their living conditions triggered by the effect of inflation on their daily needs, and decreased support and changes in the job market that led to more women working to support their families. Consequently, refugees expressed a preference towards limiting their number of children due to concern about the consequences of the crisis on their children’s physical and mental well-being. This was combined with decreased pressure on women from men and in-laws to have (additional) children and concern over the effect of Covid-19 on pregnant women.

Conclusions
The sudden deterioration in living conditions due to the overlapping crises may have influenced Syrian refugees’ preferences towards limiting their number of children or delaying having children until the situation improves. The potential shift in power dynamics in households caused by more women working outside the home also may have increased women’s autonomy in making decisions regarding family size and use of modern contraception. These findings have implications for developing programs that focus on female livelihoods and engagement in work outside the home to influence family size and other reproductive health outcomes and gender equity indicators.
Gene fusion as an important mechanism to generate new genes in the genus Oryza
Authors: Zhou Yanli , Zhang Chengjun , Zhang Li , Ye Qiannan , Liu Ningyawen , Wang Muhua , Long Guangqiang , Fan Wei , Long Manyuan , Wing Rod A. 
Published: June 2022

Abstract
Background
Events of gene fusion have been reported in several organisms. However, the general role of gene fusion as part of new gene origination remains unknown.

Results
We conduct genome-wide interrogations of four Oryza genomes by designing and implementing novel pipelines to detect fusion genes. Based on the phylogeny of ten plant species, we detect 310 fusion genes across four Oryza species. The estimated rate of origination of fusion genes in the Oryza genus is as high as 63 fusion genes per species per million years, which is fixed at 16 fusion genes per species per million years and much higher than that in flies. By RNA sequencing analysis, we find more than 44% of the fusion genes are expressed and 90% of gene pairs show strong signals of purifying selection. Further analysis of CRISPR/Cas9 knockout lines indicates that newly formed fusion genes regulate phenotype traits including seed germination, shoot length and root length, suggesting the functional significance of these genes.

Conclusions
We detect new fusion genes that may drive phenotype evolution in Oryza. This study provides novel insights into the genome evolution of Oryza.
French youth trends in prevalence of overweight, obesity and underweight from 2008 to 2018
Authors: Vanhelst Jérémy , Baudelet Jean-Benoît , Thivel David , Ovigneur Hervé , Deschamps Thibault 
Published: June 2022

Abstract
Objective
Because the prevalence overweight and obesity remains high during adolescence in Europe, tracking weight status in children and adolescents is needed. We aimed to estimate French trends in the prevalence of weight status in children and adolescent from 2008 to 2018.

Results
The prevalence of overweight and obesity were higher in boys (P < 0.0001). Underweight was more prevalent in girls (P < 0.0001). In adolescents aged 16–17 years old, a stabilization was observed for overweight and obesity whereas the prevalence of underweight increased significantly in boys (P < 0.0001). For children and adolescents aged to 10–12 and 13–15 years old, the obesity and overweight decreased significantly while the underweight was increased for both sexes aged 10–12 years (P < 0.0001). Underweight increased in boys aged 13–15 years (P < 0.0001) while that a stagnation was observed in girls. These encouraging results show the permanent need to develop preventive strategies promoting an healthy active living in order to modify the lifestyle for adolescents with underweight, overweight or obesity.
Sex and race define the effects of adverse childhood experiences on self-reported BMI and metabolic health biomarkers
Authors: Leachman Jacqueline R. , Heier Kory , Lei Feitong , Ahmed Nermin , Dalmasso Carolina , Duncan Meredith S. , Loria Analia S. 
Published: June 2022

Abstract
Background
Adverse childhood experiences (ACEs) are an independent risk factor for chronic diseases, including type 2 diabetes, stroke and ischemic heart disease. However, the effect of ACEs considering sex and race are not often reported in cohorts showing multiracial composition, with power to evaluate effects on underrepresented populations.

Aim
To determine how sex and race affected the association of combined and individual ACEs with metabolic health biomarkers in the Southern Community Cohort Study (2012–2015).

Methods
Self-reported data were analyzed from ACE surveys performed during the second follow-up of a cohort comprised by over 60% of Black subjects and with an overall mean age of 60 years.

Results
BMI steadily increased with cumulative ACEs among Black and White women, but remained relatively stable in White men with ≥ 4 ACEs. Contrary, Black men showed an inverse association between ACE and BMI. Secondary analysis of metabolic outcomes showed that physical abuse was correlated with a 4.85 cm increase in waist circumference in Black subjects. Total cholesterol increased among individuals with more than 4 ACEs. In addition, increases in HbA1c were associated with emotional and maternal abuse in Black women and sexual abuse in White women.

Conclusions
BMI is strongly associated with cumulative ACEs in women regardless the race, while waist circumference is strongly associated with ACEs in Black individuals, which combined with reduced BMI may indicate increased central adiposity in Black men. Our study suggests that sex and race influence the contribution of certain ACEs to impair metabolic health.
Inverse analysis of material parameters in coupled multi-physics biofilm models
Authors: Willmann Harald , Wall Wolfgang A. 
Published: June 2022

AbstractIn this article we propose an inverse analysis algorithm to find the best fit of multiple material parameters in different coupled multi-physics biofilm models. We use a nonlinear continuum mechanical approach to model biofilm deformation that occurs in flow cell experiments. The objective function is based on a simple geometrical measurement of the distance of the fluid biofilm interface between model and experiments. A Levenberg-Marquardt algorithm based on finite difference approximation is used as an optimizer. The proposed method uses a moderate to low amount of model evaluations. For a first presentation and evaluation the algorithm is applied and tested on different numerical examples based on generated numerical results and the addition of Gaussian noise. Achieved numerical results show that the proposed method serves well for different physical effects investigated and numerical approaches chosen for the model. Presented examples show the inverse analysis for multiple parameters in biofilm models including fluid-solid interaction effects, poroelasticity, heterogeneous material properties and growth.
L-shape triple defects in a phononic crystal for broadband piezoelectric energy harvesting
Authors: Jo Soo-Ho , Yoon Heonjun , Shin Yong Chang , Choi Wonjae , Youn Byeng D. , Kim Miso 
Published: June 2022

AbstractThis study proposes a phononic crystal (PnC) with triple defects in an L-shape arrangement for broadband piezoelectric energy harvesting (PEH). The incorporation of defects in PnCs has attracted significant attention in PEH fields owing to properties such as energy localization and amplification near the defect. Several studies have been conducted to enhance output electric power of PnC-based PEH systems with single defects. However, it is susceptible to the limitations of narrow bandwidth. Recently, double-defect-incorporated systems have been proposed to widen the PEH bandwidth via defect-band splitting. Nevertheless, the PEH performance rapidly decreases in the frequency range between the split defect bands. The limitations of single- and double-defect-incorporated systems can be resolved by the incorporation of the proposed design concept, called the L-shape triple defects in a PnC. The isolated single defect at the top vertex of the letter ‘L’ compensates for the limitations of double-defect-incorporated systems, whereas the double defects at the bottom vertices compensate for the limitations of the single-defect-incorporated systems. Hence, the proposed design can effectively confine and harvest elastic-wave energy over broadband frequencies while enhancing the application of single and double defects. The effectiveness of the proposed design concept is numerically validated using the finite element method. In the case of a circular hole-type PnC, it is verified that the PnC with L-shape triple defects broadens the bandwidth, and improves the output voltage and electric power compared with those of single- and double-defect-incorporated systems. This study expands the design space of defect-incorporated PnCs and might shed light on other engineering applications of the frequency detector and elastic wave power transfer.
Quantitative comparison of anticancer drug dispersal before and after introducing appropriate preparation procedures during anticancer drug preparation
Authors: Yanagisawa Yuki , Isama Kazuo , Kurosu Tomohiro , Natsume Yoshiaki , Seino Toshikazu , Nishimura Tetsuji , Yamashita Atsushi 
Published: June 2022

AbstractBackgroundIn Japan, engineering controls for preparing injectable anticancer drugs are inadequate and compliance with appropriate preparation procedures is vital. In this study, we evaluated the effects of adherence to appropriate anticancer drug formulation and packaging procedures on reducing anticancer drug dispersal in clinical practice, especially in Japan.MethodsWe quantitatively evaluated the effectiveness of implementing procedures that were experimentally verified to help reduce the amount of anticancer drug dispersed during preparation based on procedures described in the “Anticancer Drug Preparation Manual.” The target facilities were four regional hub hospitals in the Kanto area. Contamination of sheets and gloves with 5-fluorouracil (5-FU) and gemcitabine (GEM) in a safety cabinet during formulation was evaluated using wipe tests. Subsequently, the proper preparation procedure was shown on a video, training was provided, and the wipe tests were repeated.ResultsForty-one and 39 pharmacists were engaged in drug preparation before and after intervention, respectively. 5-FU had the highest dispersal per prepared vial on the sheet before intervention. The dispersal amount per prepared vial decreased significantly (P = 0.01) after intervention. The amount of GEM dispersed before and after intervention did not differ significantly. However, the percentage of sheets below the detection limit after intervention was 62%, increasing from 46% before intervention. The amount dispersed on gloves was not significantly reduced by proper preparation technique. Although not explicitly noticeable and quantifiable, pharmacists must consider that a significant amount of anticancer drug is dispersed on gloves despite following appropriate preparation procedures.ConclusionsQuantitative amounts of anticancer drugs dispersed in the preparations of 5-FU and GEM were found in our study. The difference in the amount of contamination before and after intervention was significantly reduced only for the contamination of sheets with 5-FU. There was no decrease in the amount of glove contamination. There was also no difference between medical facilities. Despite following appropriate preparation procedures, dispersed amounts cannot be maintained below the detection limit, indicating the need for a combination of education and engineering controls.
Three-dimensionally printed biphasic calcium phosphate blocks with different pore diameters for regeneration in rabbit calvarial defects
Authors: Seo Young-Wook , Park Jin-Young , Lee Da-Na , Jin Xiang , Cha Jae-Kook , Paik Jeong-Won , Choi Seong-Ho 
Published: June 2022

Abstract

Background
Biphasic calcium phosphate (BCP) is the most frequently used synthetic bone substitutes, which comprises a combination of hydroxyapatite (HA) and beta-tricalcium phosphate (b-TCP). Thanks to the recent advances in digital dentistry and three-dimensional (3D) printing technology, synthetic block bone substitutes can be customized to fit individual defect morphologies. The diameter of the pores can influence the rate of bone formation and material resorption. The aim of this study was to compare three-dimensionally printed biphasic calcium phosphate (BCP) block bone substitutes with different pore diameters (0.8-, 1.0-, and 1.2- mm) for use in the regeneration of rabbit calvarial defects.

Methods
Four circular defects were formed on the calvaria of ten rabbits. Each defect was randomly allocated to one of the following study groups: (i) control group, (ii) 0.8-mm group, (iii) 1.0-mm group, and (iv) 1.2-mm group. All specimens were postoperatively harvested at 2 and 8 weeks, and radiographic and histomorphometric analyses were performed on the samples.

Results

Histologically, the BCP blocks remained unresorbed up to 8 weeks, and new bone formation occurred within the porous structures of the blocks. After the short healing period of 2 weeks, histomorphometric analysis indicated that new bone formation was significantly greater in the BCP groups compared with the control (
p
< 0.05). However, there were no significant differences between the groups with different pore diameters (
p
> 0.05). At 8 weeks, only the 1.0-mm group (3.42 ± 0.48 mm
2
, mean ± standard deviation) presented a significantly larger area of new bone compared with the control (2.26 ± 0.59 mm
2
) (
p
< 0.05). Among the BCP groups, the 1.0- and 1.2-mm groups exhibited significantly larger areas of new bone compared with the 0.8-mm group (3.42 ± 0.48 and 3.04 ± 0.66 vs 1.60 ± 0.70 mm
2
, respectively).

Conclusions
Within the limitations of this study, the BCP block bone substitutes can be applied to bone defects for successful bone regeneration. Future studies should investigate more-challenging defect configurations prior to considering clinical applications.
Association between ethnicity and hypertension in Northern Colombia in 2015
Authors: Smith Drew H. , Grewal Jaskaran , Mehboob Saba , Mohan Shiva , Pombo Luisa F. , Rodriguez Pura , Gonzalez Juan Carlos , Zevallos Juan , Barengo Noël C. 
Published: June 2022

Abstract
Background
Studies in the United States have shown a genetic predisposition to hypertension in individuals of African descent. However, studies on the associations between ethnic groups and hypertension in Latin America are lacking and the limited results have been inconsistent. The objective of this study is to determine whether Afro-Colombian ethnicity increases the risk of hypertension.

Methods
This study is a secondary data analysis of a cross sectional study from five provinces in Northern Colombia. Randomly selected individuals (N = 2613; age-range 18–74 years) enrolled in a health care insurance company underwent physical examinations and completed questionnaires regarding ethnicity, lifestyle, and other risk factors. Hypertension in these patients was determined. Unadjusted and adjusted logistic regression analysis were calculated to determine the association between ethnicity and hypertension.

Results
No association between Afro-Colombian ethnicity and hypertension was found (odds ratio [OR], 0.85; 95% confidence interval [CI], 0.66–1.09). As expected, people with a body mass index (BMI) of 30 or higher were at a greater risk of having hypertension (OR, 3.12; 95% CI, 2.35–4.16) compared with those with a normal BMI.

Conclusions
Findings from this study suggest no independent association between Afro-Colombian ethnicity and hypertension. Further research should focus on genotyping or socioeconomic factors such as income level.
A new algorithm for optimal process parameters based on minimum building time in additive manufacturing
Authors: Hamoud M. , Sobhi Ahmed 
Published: June 2022

Abstract
Background
Additive manufacturing method is used for manufacturing of solid three-dimensional parts. It requires less human efforts and manufacturing time for parts is less. Different process parameters such as layer thickness, building orientation, infill type, and infill percentage affect the building time, model cost, mechanical properties, and surface roughness. The presented paper develops an algorithm for adapting layers and generating tool-paths. This algorithm can improve the fabrication efficiency and geometrical accuracy in the additive manufacturing (AM) of complex models. The proposed algorithm consists of three modules that identify the optimal process parameters, named as part building orientation, layer thickness, strategy type for internal filling, and slope of the tool-path.

Results
The input is the PTS file that contains the points of the layers contour of the computer-aided design (CAD) model. All the modules for the proposed algorithm were implemented using the MATLAB R2019a programming language software. The main finding results showed that the fabrication with an adaptive layer thickness was more time-efficient. The build time was reduced up to 47.3%. The developed tool-path generation strategies (contour offset and zigzag line tool-path) can effectively balance the AM surface quality and fabrication efficiency requirements.

Conclusion
In this research, the AM users can benefit by saving the cost and time. The parts were fabricated with a high degree of accuracy, and the surface finish was suitable for determining the optimal process parameter.