bluuebunny/crossref_metadata_2025_split huggingface dataset:
A mitochondria-targeted molecular phototheranostics platform for NIR-II imaging-guided synergistic photothermal /photodynamic /immune therapy
Authors: Yang Sha , Wu Guilong , Li Na , Wang Minghui , Wu Peixian , He Yuxuan , Zhou Wei , Xiao Hao , Tan Xiaofeng , Tang Li , Yang Qinglai 
Published: September 2022

Abstract
Phototherapy is a conducive and non-invasive strategy for cancer therapy under light irradiation. Inspiringly, fluorescence imaging in the second near-infrared window (NIR-II, 1000~1700 nm) holds a great promise for imaging-guided phototherapy with deep penetration and high spatiotemporal resolution. However, most phototherapeutics still face great challenges, including complicated synthesis of agents, potential biotoxicity and unsatisfied therapeutic outcomes. Herein, a near-infrared (NIR: 808 nm) triggered molecular photosensitizer FEPT, modified with Triphenylphosphine PEGylation (PEG2000-TPP), is developed for NIR-II imaging-guided mitochondria-targeting synergistic photothermal therapy (PTT)/photodynamic therapy (PDT)/immune therapy (IMT). The mitochondria-targeting photosensitizer FEPT can produce reactive oxygen species (ROS) and hyperpyrexia upon 808 nm laser irradiation, resulting in mitochondrial dysfunction and photo-induced apoptosis via caspase-3 pathway, and further to boost highly efficient immunogenic cell death (ICD) toward outstanding anticancer immune response with the in-situ enhancement in PDT and PTT. Hence, this work provides a practicable strategy to develop a molecular phototheranostic platform for imaging-guided cancer therapy via mitochondria-targeting.
Response of cotton genotypes to water-deficit stress using drought tolerance indices and principal component analysis
Authors: Yehia Waleed , El-Hashash Essam Fathy 
Published: September 2022

Abstract
Background
Drought impacts on cotton cultivation and production are expected to worsen as a result of global warming and water-deficit stress. Drought tolerance indices and PCA analysis were used to evaluate drought stress responses in eleven cotton genotypes and fifteen indices' ability to identify drought-tolerant genotypes under normal and drought circumstances.
Results
Seed cotton yield (Kentar/Feddan) was significantly affected by genotypes, years, and their interaction (p ≤ 0.05 or 0.01) under normal and water-deficit stress conditions, according to the combined ANOVA. Except for error variance, all genetic parameters studied for seed cotton yield were higher under normal irrigation conditions than under water-deficit stress conditions. According to PCA analysis, The STI, MP, GMP, HM, ATI, SSPI, and TOL are suitable indicators and were similar in their ability to screen, rank, and detect tolerant genotypes, due to positive correlations among each other and also the highest association with seed cotton yield in both irrigation conditions. The genotypes G4, G9, and G10 (Group A) seemed to be the most drought-tolerant and cotton productive based on mean performance, GxY heatmap analysis, drought tolerance indices, and PCA analysis.
Conclusions
The results of our study, drought tolerance indices and PCA could be useful and appropriate for studying drought tolerance mechanisms and cotton yield improvement in Egypt.
Exploration of HSPC aging mechanism based upon in vitro cell modeling and MDS clinical sampling
Authors: Hu Haiyan , Song Xiangrong , Zhao Lin , Hu Lanyue , Xu Chu , Lin Yanjuan , Zhou Manying , Lian Xiaolan , Zhang Lina 
Published: September 2022

Abstract
Background The pathogenetic mechanisms of Myelodysplastic Syndromes (MDS) were undefined. Hematopoietic senescence was manifested by association with malignant myeloid blood diseases, aging and immune dysfunction. Hematopoietic stem/progenitor cell (HSPC) aging was the primary determinant of hematopoietic senescence.MethodsIn current study, we used an in vitro HSPC aging mouse model that readily enabled the gather of a large number of aging HSPCs. The followed studies covered mRNA splicing and epigenetics (H3K27me3) relevant to HSPC aging, with methods such as Cut-tag, SA-β-gal assay, CFU-mix assay, RNA-seq, and RNAi knock down (KD). ResultsThe results showed that HSPC aging associated down-regulation of SR and hnRNPs family genes and mRNA splicing inhibitor (SSA) elicited HSPC aging-like phenotype. Cut-tag assay demonstrated that HSPCs aging was linked to global decline in H3K27me3 levels, which however, was systemically up-regulated in occupying the promoter of SR family and hnRNPs family genes. In addition, HSPCs aging exhibited alterations in the splicing patterns of TSS and SKIP.ConclusionsTogether, we proposed the linkages of HSPCs aging with epigenetic repression of SR and HnRNPs genes and inhibition of mRNA splicing pathway to alter TSS and SKIP-relevant alternative splicing. Our study provided insights to the etiopathology of MDS by exposing its connections to HSPC aging.
xCT-mediated glutamate excretion in white adipocytes stimulates interferon-γ production by natural killer cells in obesity
Authors: Kim Hee-Hoon , Shim Young-Ri , Kim Ha Neul , Yang Keungmo , Ryu Tom , Kim Kyurae , Choi Sung Eun , Kim Min Jeong , Woo Chaerin , Chung Katherine Po Sin , Hong Song Hwa , Jung Jaeone , Jung Youngae , Hwang Geum-Sook , Kim Won , Kim Seok-Hwan , Eun Hyuk Soo , Seong Je Kyung , Jeong Won-Il 
Published: September 2022

Abstract

Obesity-mediated hypoxic stress underlies inflammatory responses, including interferon (IFN)-γ production by natural killer (NK) cells in white adipose tissue. However, the effects of obesity on NK cell IFN-γ production remain obscure. We demonstrate that hypoxia promotes xCT-mediated glutamate excretion and C-X-C motif chemokine ligand 12 (CXCL12) expression in hypertrophic adipocytes, leading to NK cell recruitment. Interestingly, the spatial proximity between NK cells and xCT-expressing adipocytes increases IFN-γ production by stimulating metabotropic glutamate receptor 5 (mGluR5) in NK cells, and IFN-γ further augments the expression of xCT and CXCL12 in adipocytes. Consequently, this vicious cycle promotes adipose tissue expansion and inflammation. In contrast, genetic or pharmacological inhibition of xCT, mGluR5, or IFN-γ receptor in adipocytes or NK cells combats obesity and hepatic steatosis and improves glucose tolerance in male mice. Our findings posit that adipose glutamate-induced activation of mGluR5 in NK cells is a novel therapeutic target in obesity-related metabolic disorders.
An efficient approach for large-scale image-to-video retrieval with convolutional neural network features
Authors: Gharahsouflou Aboulfazl , Maihami Vafa , Khamforoosh Keyhan 
Published: September 2022

Abstract
In recent years, inspired by the remarkable success in deep learning, many deep neural networks (DNN)-based methods have been proposed to solve the problem by intersection analysis. These approaches, for their enormous nonlinear learning capacity, show superior performance in cross-retrieval tasks compared to traditional cross-retrieval methods. Due to the increased amount of information for information recovery and limited storage capacity, the extraction of key-frame for videos and key feature extraction is becoming a fundamental challenge. In this paper, we have investigated the image-to-video retrieval challenge, and a new convolutional neural network (CNN)-based visual search system is presented to find similar videos in an extensive database based on the target image. Our framework includes a key frame extraction algorithm and feature aggregation strategy. Specifically, in the key frame extraction algorithm, by taking advantage of the extracted objects in each image, we present a new scheme to extract the features of each form. Therefore the redundant information in the video data will be removed, and the storage cost will also be reduced to an acceptable level. The feature aggregation strategy uses deep-local-complex feature fusion, which enables fast retrieval in a large-scale video database. The results of extensive experiments on publicly available datasets demonstrate that the proposed method achieves superior performance and accuracy compared to other advanced visual search methods.
Bacteriological Profile, Culture, Sensitivity, and Associated Factors of Bacterial Conjunctivitis Among Patients Attending Eye Clinic at a Tertiary Hospital in Uganda
Authors: Mohamed Abdulsalam Ahmed , Atukunda Immaculate , Sereke Senai Goitom , Orishaba Philip , Kajumbula Henry , Nakiyingi Lydia , Rebecca Claire Lusobya 
Published: September 2022

Abstract
Background: An estimated 30 million people in Sub-Saharan Africa are affected by conjunctivitis and untreated conjunctivitis poses an increased risk of developing keratitis and corneal ulcerations which could result in blindness and visual impairment. Despite the commonality of the disease in this region, there is a paucity of studies on the occurrence and associated factors of bacterial conjunctivitis. This study sought to determine the bacteriological profile, culture, sensitivity, and factors associated with bacterial conjunctivitis among patients seen in the eye clinic at Mulago National Referral Hospital (MNRH).Methods: This was a cross-sectional study conducted among participants who presented to the eye clinic of MNRH with eye discharge, irritation, tearing, and red eyes, between January and March 2020. Findings of slit-lamp biomicroscopy were recorded. Conjunctival swabs were taken from participants for laboratory analysis to establish the bacterial profiles and antibiotic susceptibility.Results: A total of 82 participants were enrolled in the study. The median age was 30 years (IQR 16-48). Forty-two (51%) had bacterial conjunctivitis and the majority had unilateral disease (n=72, 87.8%). Participants aged 10-24.9 years had a higher prevalence of bacterial conjunctivitis compared to those aged 25 and above (aPR = 1.89, 95%CI 1.18-3.03, p = 0.008). The commonest bacteria was S. aureus (n=24, 57.1%) and were sensitive to ciprofloxacin with resistance percentages less than 20%. Almost 95% of the bacteria were susceptible to ciprofloxacin and 90% were susceptible to chloramphenicol. Conclusions: Bacterial conjunctivitis was mainly caused by gram-positive bacteria, especially S. aureus. Age was significantly associated with bacterial conjunctivitis. Most of the bacteria were sensitive to chloramphenicol, ciprofloxacin, and clindamycin.
Transcription factor EB improves ventricular remodeling after myocardial infarction by regulating the autophagy pathway
Authors: Liu Cong , Zhou DaWang , Zhang Qiang , Wei HongYan , Lu YuanZheng , Li Bo , Zhan HaoHong , Cheng JingGe , Wang ChuYue , Yang YiLin , Li ShuHao , Hu ChunLin , liao xiaoxing 
Published: September 2022

Abstract
Background Adverse left ventricular remodeling after myocardial infarction (MI) compromises cardiac function and increases heart failure risk. Till now, comprehension of the role transcription factor EB (TFEB) plays after MI is limited.ObjectivesThe purpose of this study was to describe the effects of TFEB on cell death and fibroblast differentiation after MI.MethodsAAV9 mediated up- and down-regulated TFEB expressions were generated in C57BL/6 mice two weeks before the MI modeling. Echocardiography, Masson, HE, Sirius red staining immunofluorescence, and wheat germ agglutinin staining were performed at 3 days, and 1, 2, and 4 weeks after MI modeling. Fibroblasts and myocytes collected from SD neonatal rats were transfected by adenovirus and siRNA, and cell counting kit-8 (CCK8), Cell Proliferation EdU Image (EDU), immunofluorescence, and Transwell assay were conducted. Myocardial fibrosis-related proteins and autophagy-related protein were identified by Western blot.ResultsThe up-regulation of TFEB resulted in reduced myocardial cell death, delayed fibroblasts proliferation and its differentiation into myofibroblasts, and up-regulated expression of LC3B three days after MI. Similar results were observed in vitro studies. Meanwhile, a significant up-regulation of EF, decrease in the ratio of the infarction length, and decreased protein level of collagen III were observed four weeks after MI modeling. The over-expression of TFEB slowed down myofibroblast migration and resulted in a significant down-regulation of collagen I level in myofibroblasts.ConclusionsTFEB demonstrated potential in improving cell death after MI by mediating autophagy and regulating fibroblast proliferation and transformation. Its molecular impacting mechanism deems further investigation.
Analysis of Drug Resistance Genotype and Distribution in Klebsiella pneumoniae Isolates from a Tertiary-Care Hospital in Guilin, China
Authors: Liu Zhenyu , Chen Huoying , Xiong Lijun , Wang Di , Zeng Guifen , Mo Shan , Wei Chuandong , Hou Xianliang 
Published: September 2022

Abstract
Purpose
To better understand the distribution and drug resistance of Klebsiella pneumoniae and provide a basis for clinical treatment.
Methods
We studied 167 Klebsiella pneumoniae clinical specimens in the Second Affiliated Hospital of Guilin Medical University from November 2020 to June 2022. These clinical specimens were isolated from urine, blood, sputum, catheter, pus, drainage fluid, bile, joint fluid, alveolar lavage fluid, and wound/trauma/incision secretions were collected. The drug resistance of Klebsiella pneumoniae and clinical outcomes were analyzed. We designed primers based on relevant resistance genes in The Comprehensive Antibiotic Resistance Database, while a part of them also used primer sequences from the relevant literature. We detected the carbapenems gene using polymerase chain reaction (PCR) and sent the product to the company for sequencing and homology analysis.
Results
A total of 707 inpatients were recruited and 167 isolates of Klebsiella pneumoniae were obtained. 83 strains from sputum specimens (49.7%), 18 strains from wound/trauma/incision secretion specimens (10.8%), 15 strains from pus specimens (9.0%), and other strains from urine specimens, blood specimens, drainage fluid, bile specimens, bile specimens, catheter specimens, and joint fluid specimens (30.5%). Klebsiella pneumoniae has a high rate of resistance to ampicillin (AMP) (98.2%), tetracycline (TE) (36.5%), piperacillin (PIP) (36.5%), chloramphenicol (CHL) (35.3%), and cefazolin (CFZ) (33.5%). From the analysis of sequencing results, we found that 68 strains were missing membrane pore protein Ompk-35, 3 strains were detected carrying KPC-1/KPC-2 type, 12 strains were missing membrane pore protein Ompk-36, 62 strains were missing membrane pore protein Ompk-37, 1 strain was detected carrying NDM-1, and no VIM-1, IMP-4, OXA-48, SME-2.
Conclusions
Klebsiella pneumoniae mainly causes respiratory tract infections and has a high rate of resistance to cephalosporins, piperacillin, and other antibacterial drugs. The detection rate of missing membrane pore protein ompk-35/ompk-37 is high, and the more drug resistance gene species it carries, the more drug resistant drug species it is.
Comprehensive Effects of Interdecadal Change of Sea-surface Temperature Increase in the Indo-Pacific Ocean on the “Warming-Wetting” of the Qinghai–Tibet Plateau
Authors: Dong Na , Xu Xiangde , Cai Wenyue , Zhao Tianliang , Sun Chan 
Published: September 2022

Abstract
The correlation characteristics between anomalous changes in summer precipitation on the Qinghai–Tibet Plateau (QTP) and the high-impact areas of global sea-surface temperature (SST) are mainly studied in this paper. The results show that the interdecadal change of the regional “warming-wetting” in China is especially prominent in the northern part of the main body of the QTP, which is therefore identified as the high-value area of precipitation variability. Investigations have revealed that the high-value areas of summer precipitation variability in the northern QTP are significantly correlated with four high-value areas of SST variability, namely the western North Pacific, the western Central Pacific, the Southwest Pacific, and the central Indian Ocean. In these four high-impact areas, a synchronous tendency is found in the SST increase and sea-surface specific humidity. Through the tracking analysis of the correlated vectors of the water vapor source for the warming-wetting of the QTP, it further confirms that the four high-value areas of SST variability in the Indo-Pacific Ocean are the major impact sources of water vapor transport for the warming-wetting of the QTP. Moreover, the characteristics of various interdecadal global water vapor transport circulations are compared, and the results show that from 1991 to 2020, the trans-equatorial water vapor transport from the Southern Hemisphere witnessed a notable increase, which furthermore suggests that the interdecadal change of SST increase in the Southwest Pacific and central Indian Ocean is the key reason for the warming-wetting of the QTP. In addition, a comprehensive image of high-impact marine water vapor sources for modulating the warming-wetting tendency in the QTP is proposed.
Biosynthesis of silver nanoparticles using gamma irradiated of Iranian propolis extract
Authors: Geraili Farimehr Ghanizadeh , Aghamiri Seyed Mahmoud Reza , Heidarieh Marzieh 
Published: September 2022

Abstract
Silver nanoparticles have many applications in medicine, pharmacy, and industry. Biosynthesized silver nanoparticles are suitable for medical purposes. In this study, scanning electron microscope (SEM) analysis of biosynthesized silver nanoparticles by Iranian propolis’ alcoholic extract shows that AgNPs sizes in gamma-irritated group are about 50–90 nm, and 100–580 nm in the non-irradiated group. Moreover, AgNPs in the irritated group are spherical in shape and homogeneous. DPPH analysis shows that antioxidant activity is significantly increased by raising the concentration of alcoholic extracts in both irradiated and non-irradiated states (P < 0.05). Regenerative properties of propolis alcoholic extract at both gamma-irradiated and non-irradiated states against three bacteria: Staphylococcus aureus (a Gram-positive round-shaped bacterium and food poisoning agent), E. coli (gram-negative, food poisoning agent) and B. subtilis (gram-positive), were examined. As a result, the AgNPs antioxidant properties in the irradiated group have significantly increased compared to the non-irradiated state. Studies on AgNPs antibacterial activity against Gram-positive B. subtilis and S. aureus and Gram-negative E. coli have shown that AgNPs are lethal rather than inhibitory against B. subtilis and S. aureus, and in both irradiated and non-irritated types the rate of MIC and MBC is same against Gram-negative E. coli; hence, due to green synthesis and lack of toxic substances in this method, nanoparticles can be used in medical applications.
Radical debridement and hemipelvis replantation using artificial hemipelvis prosthesis combined with anti-parasite chemotherapy in the treatment of advanced pelvic echinococcosis
Authors: Luan Haopeng , Liu Kai , Tian Qi , Chen Yuanxin , Peng Cong , Sun Xiaoyue , Song Xinghua 
Published: September 2022

Abstract

Purpose:The study aimed to access the clinical efficacy of surgery combined with anti-parasite chemotherapy in the treatment of advanced pelvic echinococcosis.Methods:From March 2005 to December 2018, the medical records and imaging of patients with pelvic echinococcosis were retrospectively evaluated, who were treated with surgery combined with anti-parasitic chemotherapy. The Enneking classification was applied to determine the location of the lesion, and the Musculoskeletal Tumor Society (MSTS) score system was used for outcome evaluation.Results:A total of 9 patients who met the criteria were included in this study, with a mean follow-up time of 4.36 ± 3.11 years. All patients were successfully treated by surgery combined with anti-parasite chemotherapy. Three patients with recurrence of pelvic echinococcosis, and the infection was under control after repeated debridement procedures. Superficial wound skin necrosis of surgical incision occurred in one case and recovered after repeated debridement.Conclusion:Surgery combined with anti-parasite chemotherapy was a practical method in the management of advanced pelvic echinococcosis. Standard anti-parasite chemotherapy played an essential role in the whole treatment.
Urease and b-glucosidase activity enhanced the transformation of functional groups of humin amended by straw and straw derived biochar
Authors: Liu Xinwei , An Qi , Song Xiangyun , Hussain Qaiser , Cui Liqiang , Wang Chengji , Wang Yan , Cui Dejie 
Published: September 2022

Abstract

Background Organic materials carbon mainly sequestrated in humin. However, the carbon (C) stability of humin with organic materials amendments in different types of soils is mainly affected by organic materials or soil types as well as the enzymatic reaction is important for understanding the C sequestration mechanism of soil. Results In this study, straw and straw-derived biochar were incorporated into three different types of soils. Using13C nuclear magnetic resonance (NMR) spectroscopy, the structural composition of humin (Hu) was analyzed, along with the urease and β-glucosidase activities. The results showed that biochar amendment enhanced aryl C to 49.63%-76.55% while straw amendments increased O-alkyl C of Hu to 33.99%-51.70%. Two-way ANOVA analysis revealed significant differences in the impact of soil types and treatments on enzyme activities and functional groups of Hu. The significant positive correlation was found between β-glucosidase activity and O-alkyl C, phenolic C, and methoxyl C of Hu. In addition, urease activity showed a significant positive correlation with alkyl C of Hu. Compared to tobacco straw, tobacco straw-biochar amendment increased β-glucosidase activity for 22.01 mg kg− 1d− 1, 26.01 mg kg− 1d− 1and 14.01 mg kg− 1d− 1in three types of soils, respectively. Conclusions These results showed that β-glucosidase contributed to the transformation of Hu functional groups and was influenced by organic materials and soil types, especially the organic materials types. The findings imply that straw or straw derived biochar application stimulates urease and β-glucosidase activities, which improves the alternation of functional groups of Hu in different types of soil.
Highly efficient and stable wide-bandgap perovskite solar cells via strain management
Authors: Yu Xuegong , Hang Pengjie , Kan Chenxia , Li Biao , Yao Yuxin , Hu Zechen , Zhang Yiqiang , Xie Jiangsheng , Wang Ying , Yang Deren 
Published: September 2022

Abstract
Wide-bandgap (WBG) perovskite solar cells (PSCs) with high performance and stability are in considerable demand in the photovoltaic market to boost tandem solar cell efficiencies. Perovskite bandgap broadening results in a high barrier for enhancing the efficiency of the PSCs and causes phase segregation in perovskite. In this study, we show that the residual strain is the key factor affecting the WBG perovskite device efficiency and stability. The DMSO addition not only helps lead halide to with opening the vertical layer spacing to form (CsI)0.08(PbI1.4Br0.6) and (CsI0.125Br0.875)0.08(PbI1.2Br0.8) intermediate phases, but also provide more nucleation sites to eliminate lattice mismatch with FAX (X = I, Br or Cl) or MAX, which dominates the strain effects on the WBG perovskite growth in a sequential deposition. By minimizing the strain, 1.67- and 1.77-eV nip devices with record efficiencies of 22.28% and 20.45%, respectively, can be achieved. The greatly enhanced suppression of phase segregation enables the device with retained 90% - 95% of initial efficiency over 4000 h of damp stability and 80% - 90% of initial efficiency over 700 h of maximum-power-point output stability under full-spectrum light without encapsulation. Besides, the 1.67-eV pin devices can achieve a competitive 22.3% efficiency while achieving considerable damp-heat, pre-ultraviolet (pre-UV) aging, and MPP tracking stability as per the tests conducted according to IEC 61215. The final efficiency for the perovskite/Si tandem is more than 28.3 %, which matches the top efficiencies reported to date.