bluuebunny/crossref_metadata_2025_split huggingface dataset:
GD2-CAR T cell therapy for H3K27M-mutated diffuse midline gliomas
Authors: Majzner Robbie G. , Ramakrishna Sneha , Yeom Kristen W. , Patel Shabnum , Chinnasamy Harshini , Schultz Liora M. , Richards Rebecca M. , Jiang Li , Barsan Valentin , Mancusi Rebecca , Geraghty Anna C. , Good Zinaida , Mochizuki Aaron Y. , Gillespie Shawn M. , Toland Angus Martin Shaw , Mahdi Jasia , Reschke Agnes , Nie Esther H. , Chau Isabelle J. , Rotiroti Maria Caterina , Mount Christopher W. , Baggott Christina , Mavroukakis Sharon , Egeler Emily , Moon Jennifer , Erickson Courtney , Green Sean , Kunicki Michael , Fujimoto Michelle , Ehlinger Zach , Reynolds Warren , Kurra Sreevidya , Warren Katherine E. , Prabhu Snehit , Vogel Hannes , Rasmussen Lindsey , Cornell Timothy T. , Partap Sonia , Fisher Paul G. , Campen Cynthia J. , Filbin Mariella G. , Grant Gerald , Sahaf Bita , Davis Kara L. , Feldman Steven A. , Mackall Crystal L. , Monje Michelle 
Published: February 2022

AbstractDiffuse intrinsic pontine glioma (DIPG) and other H3K27M-mutated diffuse midline gliomas (DMGs) are universally lethal paediatric tumours of the central nervous system1. We have previously shown that the disialoganglioside GD2 is highly expressed on H3K27M-mutated glioma cells and have demonstrated promising preclinical efficacy of GD2-directed chimeric antigen receptor (CAR) T cells2, providing the rationale for a first-in-human phase I clinical trial (NCT04196413). Because CAR T cell-induced brainstem inflammation can result in obstructive hydrocephalus, increased intracranial pressure and dangerous tissue shifts, neurocritical care precautions were incorporated. Here we present the clinical experience from the first four patients with H3K27M-mutated DIPG or spinal cord DMG treated with GD2-CAR T cells at dose level 1 (1 × 106 GD2-CAR T cells per kg administered intravenously). Patients who exhibited clinical benefit were eligible for subsequent GD2-CAR T cell infusions administered intracerebroventricularly3. Toxicity was largely related to the location of the tumour and was reversible with intensive supportive care. On-target, off-tumour toxicity was not observed. Three of four patients exhibited clinical and radiographic improvement. Pro-inflammatory cytokine levels were increased in the plasma and cerebrospinal fluid. Transcriptomic analyses of 65,598 single cells from CAR T cell products and cerebrospinal fluid elucidate heterogeneity in response between participants and administration routes. These early results underscore the promise of this therapeutic approach for patients with H3K27M-mutated DIPG or spinal cord DMG.
Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants
Authors: Welch Nicole L. , Zhu Meilin , Hua Catherine , Weller Juliane , Mirhashemi Marzieh Ezzaty , Nguyen Tien G. , Mantena Sreekar , Bauer Matthew R. , Shaw Bennett M. , Ackerman Cheri M. , Thakku Sri Gowtham , Tse Megan W. , Kehe Jared , Uwera Marie-Martine , Eversley Jacqueline S. , Bielwaski Derek A. , McGrath Graham , Braidt Joseph , Johnson Jeremy , Cerrato Felecia , Moreno Gage K. , Krasilnikova Lydia A. , Petros Brittany A. , Gionet Gabrielle L. , King Ewa , Huard Richard C. , Jalbert Samantha K. , Cleary Michael L. , Fitzgerald Nicholas A. , Gabriel Stacey B. , Gallagher Glen R. , Smole Sandra C. , Madoff Lawrence C. , Brown Catherine M. , Keller Matthew W. , Wilson Malania M. , Kirby Marie K. , Barnes John R. , Park Daniel J. , Siddle Katherine J. , Happi Christian T. , Hung Deborah T. , Springer Michael , MacInnis Bronwyn L. , Lemieux Jacob E. , Rosenberg Eric , Branda John A. , Blainey Paul C. , Sabeti Pardis C. , Myhrvold Cameron 
Published: February 2022

AbstractThe coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear need for high-throughput, multiplexed and sensitive assays for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory viruses and their emerging variants. Here, we present a cost-effective virus and variant detection platform, called microfluidic Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (mCARMEN), which combines CRISPR-based diagnostics and microfluidics with a streamlined workflow for clinical use. We developed the mCARMEN respiratory virus panel to test for up to 21 viruses, including SARS-CoV-2, other coronaviruses and both influenza strains, and demonstrated its diagnostic-grade performance on 525 patient specimens in an academic setting and 166 specimens in a clinical setting. We further developed an mCARMEN panel to enable the identification of 6 SARS-CoV-2 variant lineages, including Delta and Omicron, and evaluated it on 2,088 patient specimens with near-perfect concordance to sequencing-based variant classification. Lastly, we implemented a combined Cas13 and Cas12 approach that enables quantitative measurement of SARS-CoV-2 and influenza A viral copies in samples. The mCARMEN platform enables high-throughput surveillance of multiple viruses and variants simultaneously, enabling rapid detection of SARS-CoV-2 variants.
IGF2BP2 maybe a novel prognostic biomarker in oral squamous cell carcinoma
Authors: Wang Xiangpu , Xu Haoyue , Zhou Zuo , Guo Siyuan , Chen Renji 
Published: February 2022

Abstract
Aim: The main of the present study was to investigate the role of insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) in oral squamous cell carcinoma (OSCC) with the overarching of providing new biomarkers or potential therapeutic targets for OSCC.
Methods: We combined datasets downloaded from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and samples collected from the clinic to evaluate the expression of IGF2BP2 in OSCC. IGF2BP2 survival analysis was respectively performed based on TCGA, GEO, and clinical samples. Correlations between IGF2BP2 expression and clinicopathological parameters were then analyzed, and signaling pathways associated with IGF2BP2 expression were identified using gene set enrichment analysis (GSEA 4.1.0). Moreover, an IGF2BP2 co-expressed gene network was constructed, followed by gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis on IGF2BP2 co-expressed genes. Finally, TIMER and CIBERSORT were used to analyze the correlations among IGF2BP2, IGF2BP2-coexpressed genes, and tumor-infiltrating immune cells (TICs).
Results: IGF2BP2 was highly expressed in OSCC and significantly correlated with overall survival of OSCC patients (P&lt;0.01). High IGF2BP2 expression correlated with poor overall survival. The GSEA results showed that cell apoptosis-, tumor-, and immune-related pathways were significantly enriched in samples with high IGF2BP2 expression. Furthermore, GO and KEGG enrichment analyses results of IGF2BP2 co-expressed genes indicated that these genes are mainly associated with immunity/inflammation and tumorigenesis. In addition, IGF2BP2 and its co-expressed genes are associated with TICs (P&lt;0.01).
Conclusion: IGF2BP2 may be a potential prognostic biomarker in OSCC and correlates with immune infiltrates.
A psychological model for the prediction of energy‐relevant behaviours in buildings: Cognitive parameter optimisation
Authors: von Grabe Jörn , Korsavi Sepideh 
Published: February 2022

AbstractEnergy consumption in buildings is a major contributor to global warming and therefore has become a field of intensive research. This type of energy consumption can be described in two dimensions: an appliance‐based dimension and a behaviour‐based dimension. To address the behaviour‐based dimension a recent study proposed a cognitive human‐building interaction model that builds on the instance‐based learning paradigm. However, since the values of the standard cognitive parameters commonly used for modelling lab‐based behaviours are not suitable for the ‘real‐world’ domain of human‐building interaction, this paper aims to identify cognitive parameter values adapted to and suitable for the specific character of this application domain. To achieve this goal, a virtual test environment—consisting of an occupied room and a corresponding model task—was designed to test the performance of the model and its dependence on a set of fundamental cognitive parameters. A test criterion was developed that did not depend on empirical data but used the predictive consistency of the model as reference. A range of values was pre‐selected for each parameter based on theoretical and empirical considerations, which was then tested against the evaluation criterion. The performance of the model was improved significantly throughout the parametrisation process and yielded plausible results.
A novel cell structure‐based disparity estimation for unsupervised stereo matching
Authors: Cheng Xianjing , Zhao Yong , Yang Wenbang , Hu Zhijun , Yu Xiaomin , Zhao Haoliang , Zeng Pengcheng 
Published: February 2022

AbstractIt is well known that preserving depth edges is an effective solution for achieving the accurate disparity map in stereo matching, but many state‐of‐the‐art methods do not preserve depth edges well. In order to solve it efficiently, the cell structure containing irregular and regular shape regions is designed to preserve depth edges. Based on the well‐designed cell structure, a novel disparity estimation method for stereo matching is proposed, in which a two‐layer disparity optimization method is proposed to refine the disparity plane; it includes the front‐parallel disparities computation and slanted‐surfaces disparity plane refinement. In the framework of front‐parallel disparities computation, a tree‐based cost aggregation method is presented to make full use of the segmentation information of cells and then performing semi‐global cost aggregation. In the framework of slanted‐surfaces disparity plane refinement, a new probability model is proposed that employs Bayesian inference for refining disparities in textureless, weak texture and occluded regions. Experimental results show that higher accuracy could be achieved via the proposed method compared with some known state‐of‐the‐art stereo methods on KITTI 2015 and Middlebury dataset, which are the standard benchmarks for testing the stereo matching methods. It can also be indicated that the proposed method can produce accurate disparity map and have good generalization performance.
miR-551b is Associated with the Poor Prognosis and Malignant
Development of Papillary Thyroid Cancer Through Regulating ERBB4
Authors: Wang Jian , Liu Haibo 
Published: February 2022

AbstractThe function of miR-551b has been widely reported in various human cancers, and
its dysregulation in papillary thyroid cancer (PTC) has also been disclosed,
implying its potential regulator role in PTC. The aim of the study was to
evaluate the function of miR-551b in PTC development and its potential
mechanism. miR-551b was evaluated in PTC tissues and cells by RT-qPCR and
associated with the clinicopathological features of patients. The biological
effect of miR-551b on cellular processes of PTC was assessed with the CCK8
proliferation assay and the Transwell migration and invasion assay. The
potential molecular mechanism was estimated with the dual-luciferase reporter
assay. miR-551b was significantly upregulated in PTC, which showed a close
relationship with the malignancy and development of PTC patients. miR-551b
served as a prognostic biomarker negatively related to patients’
survival together with the TNM stage. The overexpression of miR-551b exerted
promoted effect on the development-related cellular processes of PTC, which was
reversed by the overexpression of ERBB4. In conclusion, miR-551b could predict
the poor prognosis of PTC patients and serve as a tumor promoter via suppressing
ERBB4.
Drug Combinations for Mood Disorders and Physical Comorbidities That
Need Attention: A Cross-Sectional National Database Survey
Authors: Kuramochi Shin , Yatomi Taisuke , Uchida Takahito , Takeuchi Hiroyoshi , Mimura Masaru , Uchida Hiroyuki 
Published: February 2022

Abstract
Introduction This study investigated combined prescriptions of drugs for
mood disorders and physical comorbidities that need special attention in the
light of frequent physical comorbidities in patients with mood disorders.
Methods We used the claims sampling data of 581,990 outpatients in January
2015 from the National Database of Health Insurance Claims and Specific Health
Checkups of Japan. Fisher’s exact test was performed to compare the
prescription rates of non-steroidal anti-inflammatory drugs (NSAIDs),
loop/thiazide diuretics, angiotensin-converting enzyme inhibitors,
and/or angiotensin II receptor blockers between lithium users and age-
and sex-matched non-lithium users; NSAIDs, antiplatelet drugs, and/or
anticoagulants between selective serotonin reuptake inhibitor
(SSRI)/serotonin-noradrenaline reuptake inhibitor (SNRI) users and
non-users; warfarin between mirtazapine users and non-users; and the proportions
of patients in the two groups with a diagnosis of somatic conditions for which
these medications were indicated and actually received them. A Bonferroni
corrected p-value of<0.05/3 was considered statistically
significant.
Results Prescriptions of the above-mentioned medications were less
frequent in lithium and mirtazapine users and comparable in SSRI/SNRI
users, compared to non-users (18.3 vs. 31.9%,
p=7.6×10−10; 0.78 vs. 1.65%,
p=0.01; 23.1 vs. 24.1%, p=0.044). In a subgroup of
patients with somatic diseases for which these medications were indicated, the
prescription rates were comparable in lithium and mirtazapine users and higher
in SSRI/SNRI users compared to non-users (28.0 vs. 29.4%,
p=0.73; 4.7 vs. 7.4%, p=0.28; 35.6 vs. 33.4%,
p=0.0026).
Discussion Pharmacotherapy with drugs for mood disorders and physical
comorbidities that require attention was commonly observed in clinical
practice.
Risk Factors and Functional Outcomes with Early Neurological Deterioration after Mechanical Thrombectomy for Acute Large Vessel Occlusion Stroke
Authors: Liu Hongwei , Zhang Yi , Fan Haixia , Wen Chao 
Published: February 2022

AbstractEarly neurological deterioration (END) is associated with a poor survival after mechanical thrombectomy (MT) in acute ischemic stroke (AIS). To assess risk factors and functional outcomes of END after MT in patients, we analyzed data from 79 patients who received MT with large-vessel occlusion. END after MT in patients is defined as an increase of two points or more in the National Institute of Health Stroke Scale (NIHSS) score, compared with the best neurological status within 7 days. The mechanism of END can be classified into: AIS progression, sICH, and encephaledema. A total of 32 AIS patients (40.5%) had END after MT. Risk factors for END after MT included: history of oral antiplatelet and/or anticoagulation drugs before MT (OR = 9.56,95% CI = 1.02–89.57), higher NIHSS score when admitted to hospital (OR = 1.24, 95% CI = 1.04–1.48), under the subtype of atherosclerotic stroke (OR = 17.36, 95% CI = 1.51–199.56), ASITN/SIR< 2 (OR = 15.78, 95% CI = 1.65–151.26), and prolonged period from AIS onset to the first revascularization (OR = 1.01, 95% CI = 1.00–1.02). AIS patients who had END at early stages were more likely to experience poor outcomes (Modified Rankin Scale [mRS] >2) at 90 days after MT (OR = 6.829, 95% CI = 1.573–29.655). Thus, AIS patients who had experienced END at early stages were more likely to have poor outcomes (mRS >2) at 90 days after MT, and the risk factors of END were connected to the mechanism of END.
Real-World Breast Cancer Patient Follow-Up Practices by Medical Oncologists in India—A Survey Report
Authors: Patel Amol , Gupta Vineet Govinda , Guleria Bhupesh , Das Chandan K. , Mehta Prashant , Ramegowda Kaushik Mahadevapura 
Published: February 2022

Background In India, breast cancer patients' post-treatment follow-up practices are not known. We did this survey to understand how the breast cancer patients are followed-up and tried to explore the challenges associated with it.
Methods We conducted a survey-based study among Indian oncologists. Seven questions were framed pertaining to follow-up practices. Answers were provided in the form of multiple options. Google forms platform was used. Survey was circulated through social media apps and through mail. We sought suggestions and opinions to address the challenges from participants.
Results A total of 158 medical oncologists responded to this survey. 10% were not aware that only history and clinical examination are the scientific recommendations for follow-up. Ninety percent of the medical oncologists felt clinical breast examination as an uncomfortable practice for patients and physicians and 39% ordered a chest X-ray and an ultrasound abdomen. Annual mammogram was ordered by 83%, and blood investigations were recommended by 14% routinely. The majority (49.6%) felt that the absence of a female attendant, physician and patient factors were responsible for nonadherence to clinical breast examination. The DEXA scan was recommended by 84 (53%) medical oncologists regularly for patients on aromatase inhibitors, while 23 (14%) did not recommend it.
Conclusion There is a disparity between scientific recommendations and real-world follow-up practices. A large number of medical oncologists relied on chest X-ray and ultrasound abdomen. There is an unmet need to address this issue.
A Structural, Cognitive, and Behavioral Model for Error Analysis of Group B Streptococcus Prophylaxis in Pregnancy
Authors: Murphy Robert E. , Ibekwe Jane C. , Ibekwe Stella I. , Refuerzo Jerrie S. 
Published: February 2022

AbstractThe objective of this study was to develop a structural-cognitive-behavioral model for error analysis of group B streptococcus (GBS) prophylaxis failure, classify delivery cases into this model, and examine compliance with treatment guidelines. A retrospective, cohort study was conducted of women with liveborn pregnancies greater than 24 weeks in April 2018 at a single hospital. We created a structural-cognitive-behavioral model of five assessments for adherence to GBS prophylaxis guidelines and then classified these into four distinct error stages. A descriptive analysis was performed to determine if the pregnancy had a perfect process, a GBS prophylaxis failure, or a fortuitous outcome. There were 313 women who met the study criteria. The rate of GBS positive was 12.8%, negative 37.4%, and unknown 49.8%. The most common errors were cognitive perception errors related to incorrectly documenting GBS status, 57.7% (N = 79). Of these errors, 15.2% (N = 12) led to GBS prophylaxis failure. Perfect outcomes occurred in 62.7% (N = 196) women, GBS prophylaxis failure occurred in 13.7% (N = 43), and fortuitous outcomes occurred in 23.6% (N = 74). In our study, we were able to identify structural, cognitive, and behavioral errors that contribute to GBS prophylaxis failures. In other cases, these errors may contribute to fortuitous outcomes.
Contradições diagnósticas na síndrome do túnel do carpo
Authors: Paiva Filho Henver Ribeiro , Costa Antonio Carlos , Paiva Valdênia Graças Nascimento , Severino Nilson Roberto 
Published: February 2022

Resumo
Objetivo Diante da divergência sobre a necessidade de exames complementares, como ultrassonografia (US) e eletroneuromiografia (ENMG) para o diagnóstico da síndrome do túnel do carpo (STC), objetivamos elucidar qual deles apresenta maior precisão na confirmação da presença ou não desta afecção.
Métodos Um total de 175 pacientes de um ambulatório de cirurgia da mão foram avaliados clinicamente, e os resultados dos testes clínicos (Tinel, Phalen e Durkan), da US (normal ou alterada) e da ENMG (normal, leve, moderada e grave) foram anotados, cruzados, e submetidos a análise estatística para verificar a concordância entre eles.
Resultados A idade média da amostra era de 53 anos, sendo prevalente o sexo feminino (159 casos). Dos pacientes com teste clínico positivo, 43,7% apresentavam US normal, e 41,7%, ENMG sem alterações. Foram encontrados resultados negativos no Tinel em 46,9% no Phalen em 47,4%, e no Durkan em 39,7%. No cruzamento entre a ENMG e os demais métodos diagnósticos, houve pouca concordância estatística.
Conclusão Não houve concordância entre os resultados dos exames clínicos, da US e da ENMG no diagnóstico da STC, e não há exame clínico ou complementar para STC que determine a conduta terapêutica com precisão.
Nível de Evidência IV, Série de Casos.
Complications of Transcutaneous Protheses – A Systematic Review of Publications Over the Past 10 Years
Authors: Souza Marcos Antonio de , Vallejos Riart Santiago Luis , Souza Stephanie Rugeri de , de Brito Rubens , Bento Ricardo Ferreira 
Published: February 2022

AbstractIntroduction Bone conduction implants, responsible for transmitting sound from an external processor to the inner ear, can be divided into active and passive, depending on the vibratory stimulus location. The use of transcutaneous device has increased, given its aesthetic appeal, the complications and limitations of percutaneous devices, and patient's treatment adherence, focusing mainly on efficacy. However, various complications are associated with the use of transcutaneous prosthesis, which can often be serious.Objective To approach the literature on complications involving transcutaneous bone-anchored prostheses through a systematic review of articles published in the past 10 years (2011–2021).Data Synthesis The MEDLINE, EMBASE, Scopus, and Cochrane Library databases were searched. All articles written in English reporting on currently available transcutaneous prosthesis implantation and its complications were selected. Studies on both children and adults were included. The data on complications were extracted, and complications were classified as minor or major and associated to each device used. Thirty-seven articles were included in the study, of which 14 were prospective cohort studies, 22 were retrospective case series, and 1 was a case report. Most studies (18) included both adults and children. Moreover, 901 implantations were performed, of which 552 implanted Baha Attract (Cochlear Ltd., Sydney, Australia), 244 implanted BoneBridge (MED-EL, Innsbruck, Austria), and 105 implanted Sophono (Sophono Inc., Boulder, CO, USA]). Furthermore, 192 adverse events were reported (total complication rate, 21.3%), with 161 minor complications (84.3%) and 31 major complications (16.1%).Conclusion Transcutaneous prosthesis is an audiological alternative with fewer complications than percutaneous prosthesis. However, its indication should be judicious because complications are common, and although most complications are minor, serious infections requiring explantation may develop.
Shall we forget human nature? Political anthropology and technics from Marx and Engels to Simondon
Authors: Bardin Andrea , Raimondi Fabio 
Published: February 2022

AbstractHuman nature is something of a taboo on the left wing of contemporary political theory and scarcely more than a commonsense assumption on its right wing. This article aims to expose the taboo and to challenge the assumption. There is no way, we argue, to defeat conservative political theory without delving into political anthropology. With this purpose in mind, our article analyses the writings of Marx and Engels, and Simondon’s concepts of the transindividual and technics. It shows that Simondon’s theory of technics allows new interpretations of many of the themes scattered in Marx’s and Engels’s works and helps to formulate a materialist political anthropology which entails a political project of liberating ‘human nature’ from labour.