bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening, optimization and molecular characterization of cold-active lipase producing Bacillus cereus I13 from Arctic sediments
Authors: KATTATHEYIL HAFSA , SAJEELA VISHNUPRIYA , SALEENA SHAHANA KABEER , EZHUTHANIKKUNNEL AKHIL PRAKASH , PADINCHATI KRISHNAN KOTTEKATTU , AMMANAMVEETIL MOHAMED HATHA ABDULLAH 
Published: November 2022

Abstract

Cold active enzymes are valuable biocatalysts, due to their capacity to withstand extreme conditions. Microbial lipases are widely used in biotechnological applications as well as in industries due to their broad substrate specificity, and higher stability with less production costs compared to other sources. In the present study, bacterial strains were isolated from the surface and sub-surface sediment samples of the Arctic fjord and were screened for lipase enzyme production. From the 73 isolates that were screened, 8 isolates are considered to be good lipase producers. Among the 8 isolates, Bacillus cereus I13 produced lipase with the activity of 11.42 U/ml on the 4th day of incubation at 20 °C along with the highest zone of clearance of 27 mm in the agar well diffusion plate method. The optimum condition for higher lipase production was at pH 7.0, a temperature of 20 °C, an incubation period of 96 hours with glucose as a carbon source, yeast as the nitrogen source, and olive oil as substrate. Bacillus cereus I13 from Arctic fjord sediments can be used as a potential candidate for the industrial production of cold-active lipase. The optimization studies described in the study proved that lipase production can be effectively increased using these potent isolates.
The therapeutic monoclonal antibody bamlanivimab does not enhance SARS‑CoV-2 infection by FcR-mediated mechanisms
Authors: Cross Robert M , Wiethoff Christopher M , Brown-Augsburger Patricia , Berens Shawn , Blackbourne Jamie , Liu Ling , Wu Xiaohua , Tetreault Jonathan , Dodd Carter , Sina Ramtin , Witcher Derrick R , Newcomb Deanna , Frost Denzil , Wilcox Angela , Borisevich Viktoriya , Agans Krystle N , Woolsey Courtney , Prasad Abhishek N , Deer Daniel J , Geisbert Joan B , Dobias Natalie S , Fenton Karla A , Strifler Beth , Ebert Philip , Higgs Richard , Beall Anne , Chanda Sumit , Riva Laura , Yin Xin , Geisbert Thomas W 
Published: November 2022

Abstract

Background Antibodies targeting envelope glycoproteins have been shown in some instances to enhance infection by subverting Fc receptor and complement function, or by directly inducing fusion with cellular membranes. The potential for antibody dependent enhancement (ADE) of infection raises concern that passive immunization with a therapeutic anti-viral antibody could increase risk of disease. As part of the nonclinical package characterizing the risk profile of the SARS-CoV-2 neutralizing monoclonal antibody bamlanivimab, studies were conducted to evaluate the potential for ADE of infectionin vitroand in a non-human primate model of COVID-19. MethodsIn vitroassays were performed in primary human macrophage, Raji, or THP-1 cells exposed to SARS-CoV-2 in the presence of bamlanivimab ranging from approximately IC50 to more than 100-fold above or below the IC50. Samples were evaluated for demonstration of productive viral infection. Bamlanivimab binding to C1q and FcR were quantified, and activity was studied by cell-based assays.In vivostudies were performed in African green monkeys (AGM) infected with SARS-CoV-2 virus following sub-saturating or saturating doses of bamlanivimab or IgG control. Viral loads, clinical pathology, and histology endpoints were assessed to determine if bamlanivimab enhanced SARS-CoV-2 replication or clinical illness. Mixed model repeated measures were used to evaluate virology statistics. Results Bamlanivimab did not increase viral RNA production in FcγR-expressing cell lines, despite demonstration of effector function. No significant differences were found among the AGM groups in terms of weight, temperature, or food intake. Treatment with bamlanivimab reduced viral loads in nasal and oral swabs and BAL fluid relative to control groups. Viral antigen was not detected in lung tissue from animals treated with the highest dose of bamlanivimab. Microscopic findings along with decreases in viral loads in bamlanivimab-treated animals indicated that ADE of disease was not observed in this study. Conclusions Sub-saturating doses of bamlanivimab treatment do not induce ADE of SARSCoV2 infection in eitherin vitroor an AGM model of infection. Findings suggest that high affinity monoclonal antibodies pose a low risk of mediating ADE in patients and further supports their safety profile as a treatment of COVID-19 disease.
A Signature of Cuproptosis-Related lncRNAs Predicts Prognosis and Provide Basis for Future Anti-tumor Drug Development in Breast Cancer
Authors: Yu Hao , Liu Yanbiao , Zhang Wenrong , Peng Ziqi , Yu Xinmiao , Jin Feng 
Published: November 2022

Abstract
Background
Breast cancer is the most prevalent malignancy worldwide and the leading culprit for women death. Cuproptosis is a novel and promising modality of tumor cell death whose relationship with lncRNAs remains shrouded in veil. Studies in cuproptosis-related lncRNAs can aid on the clinical management of breast cancer and provide basis for anti-tumor drug development.
Methods
RNA-Seq data, somatic mutation data and clinical information were downloaded from The Cancer Genome Atlas (TCGA). Patients were divided into high and low risk groups according to the risk score they got. Cox regression and LASSO regression analyses were used to select prognostic lncRNAs to construct a risk score system. Its’ prognostic value was confirmed in the training and validation cohorts subsequently. Functional analysis regarding cuproptosis-related lncRNAs was performed.
Results
Eighteen cuproptosis-related lncRNAs were identified and 11 of them including AL023882.1, AC091588.1, AC138028.2, AC027514.1, AL592301.1, LRRC8C-DT, MFF-DT, NIFK-AS1, MECOM-AS1, OTUD6B-AS1 and RNF32-AS1 were selected for risk score system construction. The risk score was confirmed as an independent prognostic factor and patients in the high-risk group had worse prognosis. A nomogram based on the independent prognostic factors was constructed for clinical decision aids. Further analyses revealed that patients in the high-risk group faced a heavier tumor mutation burden and suppressed anti-tumor immunity. Besides, cuproptosis-related lncRNAs were associated with expression of immune checkpoint inhibitors, m6a and drug sensitivity in breast cancer.
Conclusions
A prognostic risk score system with satisfactory predictive accuracy was constructed. Besides, cuproptosis-related lncRNAs can influence immune microenvironment, TMB, m6a and drug sensitivity in breast cancer, which may provide basis for future anti-tumor drug development.
Characterizing Urban Growth and Land Surface Temperature in the Western Himalayan Cities of India using Remote Sensing and Spatial Metrics
Authors: Gupta Rajman , Sharma Mani , Singh Garima , Joshi Rajendra Kr 
Published: November 2022

Abstract

Urban heat island (UHI) is developing in major cities of India due to increased urbanization and decreased vegetation cover. Urbanization induces substantial land use changes resulting in surface transformation and thermal emissions which modifies urban microclimate posing serious risk to human health, environment and ecology of the Himalayan ecosystems. Therefore, an improved understanding is required to mitigate UHI. The study illustrates the impact of urbanization on land surface temperature (LST) in the capital city- Shimla and Dehradun in the western Himalayan, India using satellite datasets. The process has been analyzed using urban cover patterns derived from Landsat 5, 7 and 8 with respective sensors of TM, ETM+, and OLI. Built-up and non-built-up areas were extracted and biophysical parameters NDVI, NDBI, NDWI and LST were computed to capture different characteristics of urban growth. The result indicated that during study period, built-up areas have increased from 32.19 Km2 (2000) to 68.37 Km2 (2016) for Dehradun and 12.38 Km2 (2000) to 29.47 Km2 (2016) for Shimla which has manifested an increase in NDBI and LST and decrease in NDVI and NDWI. The results revealed that temperature hotspots were maximum in built-up areas followed by vegetation and water body. We observed significant correlation (p < 0.05) between LST and biophysical parameters -NDVI, NDBI, NDWI. Spatial metrics at class and landscape-level has indicated that landscape has fragmented, became more heterogeneous from 2000 to 2016 because of increased urban growth. The trends identified for changes in landscape patterns and their ramifications in respective areas have prospective policy implications.
A novel low-cost bearing fault diagnosis method based on convolutional neural network with full stage optimization in strong noise environment
Authors: Jiang Li , Yu Zhipeng , Zhuang Kejia , Li Yibing 
Published: November 2022

Abstract
In recent years, convolutional neural network (CNN) has been successfully applied in the field of bearing fault diagnosis. So as to improve the diagnosis performance in harsh environment with strong noise, the structure of CNN-based feature extractor becomes deeper and more complex. However, with the increase of depth, the model may lose shallow features and the training parameters will surge. Moreover, if the sample size is not large, it tends to over fit. It deviates from the concept of network lightweight. On the other hand, little attention will be paid to the optimization of model classifiers which can significantly improve the classification performance. Therefore, we proposed a CNN with full stage optimization (FSOCNN) model for bearing fault diagnosis in strong noise environment. In the feature extraction stage, the model is optimized with a novel multi-feature output structure connected with global average pooling to improve the feature extraction ability without any extra trainable parameters. In the classification stage, the traditional softmax layer will only participate in the parameter optimization of CNN model through gradient descent algorithm, and the diagnosis results will be output by support vector machine. The effectiveness of the proposed method is verified on the two bearing datasets under different levels of noise. Compared with the existing five fault diagnosis models, the results prove that the proposed method possesses higher accuracy, less training time, and better stability.
Giant thoracic cyst caused by amniotic fluid aspiration during delivery
Authors: Wang Yifei , Sun Qi , Huang Yiling , Chen Rong , Wang Mingdong , Zhao Xuewei 
Published: November 2022

Abstract
In infants born after term, it is not unusual to find aspirated amniotic fluid (AF), but the long term effect on lung morphology and thoracic cavity is still rarely reported. Here, we report a case of a giant fibrocystic thoracic cavity with pulmonary dysplasia in adulthood, possibly due to aspiration of meconium-contaminated amniotic fluid during delivery. The patient was a 20-year-old girl presenting with chest tightness and recurrent cough for 4 months (until the time of arriving our hospital). When she was born, she had a lung infection due to aspiration of amniotic fluid contaminated with meconium, so she was admitted to the neonatal intensive care unit for rescue. Image examinations confirmed a giant thoracic cavity cyst with pulmonary dysplasia in the left thoracic cavity. The patient underwent a thoracoscopic exploratory operation in the Nantong First People's Hospital on February 26, 2021, but the thoracic cyst still existed after the operation, and the patient's symptoms did not improve. She arrived to our hospital on April 21, 2021. On April 28, 2021, she underwent the urgent left thoracic giant cyst resection surgery for intrathoracic hemorrhage. The patient's symptoms improved significantly after the operation, and the patient recovered and was discharged from the hospital smoothly. Four months later, due to the left pneumothorax attack, she returned to our hospital and underwent the left lung repair operation under thoracoscopic. The re-examination result showed that the current left lung recruitment was satisfactory, and the follow-up result showed that everything in daily life is normal. The perinatal inhalation of meconium-and/or blood-stained amniotic liquid is a life-threatening event requiring intensive care. Aspiration of meconium-contaminated amniotic fluid can induce exudative alveolitis, coupled with airway obstruction and degradation of alveolar surfactant, resulting in atelectasis. In addition, amniotic fluid and meconium could disperse from the terminal bronchioles into the alveolar acini and pleural tissue causing pleural thickening. Amniotic fluid contains a variety of mesenchymal stem cells as well as growth factors, especially basic fibroblast growth factor (bFGF), which, in the context of inflammation, ultimately lead to the formation of giant thoracic cyst. In the Discussion section, we conducted an in-depth analysis of the relationship between the amniotic fluid aspiration at birth and its eventual pathological changes in this patient through a literature review.
Evaluating hospital consumer friendly price-estimator tools for shoppable services within dermatology
Authors: Yang Kevin , Drake Lara , Ghatnekar Shilpa , Kassamali Bina , Nambudiri Vinod E. 
Published: November 2022

Abstract

A recent mandate by the Centers for Medicare and Medicaid Services (CMS) required hospitals to publish prices online. This study examined the utility of CMS-mandated price estimators for dermatology procedures. Chargemasters and price-estimator tools from the five largest hospitals in each state (n=250) were reviewed for accessibility. Price estimators were also searched for the presence of dermatologic procedures and their estimated prices. Of the 250 hospitals included, 89.6% of chargemasters and 92.8% of price estimators were accessible. Of the available chargemasters and price estimators, 98.7% and 63.8% contained pricing data on dermatologic procedures, respectively. The most common dermatology procedures listed were debridement of subcutaneous tissue (CPT 11042) with a median price of $1,135.50 (IQR $577-$3533.67; n= 80), followed by simple incision and drainage of skin abscess (CPT 10060) with a median price of $956.00 (IQR $465.50-$6506.00, n=79), and single punch biopsy of skin (CPT 11104) with a median price of $506.06 (IQR $356.50-$922.08, n=29). Although price estimator tools are widely available in patient-friendly and searchable formats, they are not optimized for shoppable dermatologic services. Adding more dermatologic procedures to patient friendly price-estimator tools can help patients directly realize savings from price shopping.
N-acetylcysteine protects against neurodevelopmental injuries induced by methylmercury exposure during pregnacy and lactation
Authors: Li Xiaoyang , Pan Jingjing , Liu Haihui , Wang Chen , Xu Si , Xu Bin , Deng Yu , Yang Tianyao , Liu Wei , Sun Jingyi 
Published: November 2022

Abstract
As an extremely dangerous environmental contaminant, methylmercury (MeHg) results in detrimental health effects in human brain nervous system, one of its main targets. However, as a developmental toxicant, the brain of offspring is vulnerable to MeHg during pregnancy and lactation exposure. Unfortunately, mechanisms of neurodevelopmental injuries induced by MeHg have not been fully elucidated. N-acetylcysteine (NAC) has been used for several decades as an antioxidant to antagonize oxidative stress. However, the molecular mechanisms of NAC alleviating MeHg-induced neurodevelopmental toxicity are not clear. Here, for evaluation of the dose-dependent effects of MeHg exposure on neurodevelopmental injuries of offspring, and the possible protective effects of NAC, the pregnant female mice were exposed to MeHg and NAC from gestational day 1 (GD1) to postnatal day 21 (PND21). Our results indicated that administering MeHg caused behavioral impairment and neuronal injuries in the cerebral cortex of newborn mice. MeHg dose-dependently caused reactive oxygen species (ROS) overproduction and oxidative stress aggravation, together with expression of Nrf2, HO-1, Notch1, and p21 up-regulation, and CDK2 inhibition. NAC treatment dose-dependently antagonized MeHg-induced oxidative stress that may contribute to alleviate the neurobehavioral and neurodevelopmental impairments. These results give insight into that NAC can protect against MeHg-induced neurodevelopmental toxicity by its antioxidation capacity.
Bioactivity and genome analysis of Bacillus amyloliquefaciens GL18 isolated from the rhizosphere of Kobresia myosuroides in an alpine meadow
Authors: Chen L. , Xie Y. L. , Wu X. H. , Wu L. L. , Yang J. , Gao Y. , Mi Y. , Yang F. 
Published: November 2022

Abstract
The unique eco-environment of the Qinghai–Tibet Plateau breeds abundant microbial resources. In this research, the antagonistic activity, bacteriostatic hydrolase activity, and low temperature, salt, and drought resistance of Bacillus amyloliquefaciens GL18, isolated from the rhizosphere of Kobresia myosuroides from an alpine meadow, were determined and analysed. The seedlings of Avena sativa were root-irrigated using bacteria suspensions (cell concentration 1 ⋅ 107 cfu/mL) of GL18, and the growth-promoting effect of GL18 on it was determined under cold, salt and drought stress, respectively. The whole genome of GL18 was sequenced, and its functional genes were analysed. GL18 presented significant antagonistic activity to Fusarium graminearum, Fusarium acuminatum, Fusarium oxysporum and Aspergillus niger (inhibition zone diameter > 17 mm). Transparent zones formed on four hydrolase detection media, indicating that GL18 secreted cellulase, protease, pectinase and β-1, 3-glucanase. GL18 tolerated conditions of 10°C, 11% NaCl concentration and 15% PEG-6000 concentration, presenting cold, salt and drought resistance. GL18 improved the cold, salt and drought tolerance of A. sativa and it showed significant growth effects under different stress. The total length of the GL18 genome was 3,915,550 bp, and the number of CDS was 3726. Compared with the COG, GO and KEGG databases, 3088, 2869 and 2357 functional genes were annotated, respectively. GL18 contained gene clusters related to antibacterial substances, functional genes related to the synthesis of plant growth-promoting substances, and encoding genes related to stress resistance. This study identified an excellent Bacillus strain and provided a theoretical basis for improving stress resistance and promoting the growth of herbages under abiotic stress.
A journey to unravel the pathophysiology of stable and exacerbated Chronic obstructive pulmonary disease through erythrocyte proteomics: A combined mass spectrometry/bioinformatics approach
Authors: Das Sonu , John Mathew , Maria Evelyn , M Vanditha , Adiody Supriya , Varghese Jinsu 
Published: November 2022

Abstract
Chronic Obstructive Pulmonary Disease (COPD) is a progressive lung disorder with high mortality. The present study, explores the novel and highly enriched protein networks differentially expressed in stable and exacerbated COPD variants to elucidate the disease pathophysiology. A label free relative quantification of erythrocyte cytosol proteome based on LC-MS/MS was performed on hemodepleted erythrocyte lysate samples of stable and exacerbated COPD with respect to healthy controls.
Five highly enriched protein clusters in stable and seven in exacerbated COPD were observed, on the application of MCODE algorithm and hierarchical clustering. Functional annotation and over-representation analysis (ORA) of the differentially expressed proteins brought to light the dysregulation of molecular events such as ERAD pathway, MAPK signalling, ciliogenesis, hypoxia, apoptosis and neutrophil migration resulting in the chronic inflammatory response characteristic to COPD. This study is a first-time report of the differential expression of unique proteins such as kyphoscoliosis peptidase, sperm associated antigen-1, calpastatin and LINE-1 in exacerbated COPD, which would lead to chronic bronchitis, bronchiectasis and bacterial or viral infections, contributing to increased severity in exacerbated COPD. The identified proteins could serve as potential candidates for biomarkers in unravelling the pathophysiology of COPD.
Enhanced Recovery Pathway in Open and Minimally Invasive Colorectal Cancer Surgery: A Prospective study on Feasibility, Compliance and Outcomes
Authors: Thomas Martin , Agarwal Vandana , DeSouza Ashwin , Joshi Riddhi , Mali Minal , Panhale Karuna , Salvi Omkar K. , Ambulkar Reshma , Shrikhande Shailesh , Saklani Avnish 
Published: November 2022

Abstract
Background
Enhanced recovery program (ERP) has demonstrated improved postoperative outcomes with increased adherence to pathway. However, there is scarce data on feasibility and safety in resource limited setting. The objective was to assess compliance with ERP and its impact on postoperative outcomes and return to intended oncological treatment (RIOT).
Methods
A single center prospective observational audit was conducted from 2014–2019, in elective colorectal cancer surgery. Before implementation, multi-disciplinary team was educated regarding ERP. Adherence to ERP protocol and its elements was recorded. Impact of quantum of adherence (≥ 80% vs. <80%) to ERP on postoperative morbidity, mortality, readmission, stay, re-exploration, functional GI recovery, surgical-specific complications, and RIOT was evaluated for open and minimal invasive surgery (MIS).
Results
During study, 937 patients underwent elective colorectal cancer surgery. Overall Compliance with ERP was 73.3%. More than 80% compliance was observed in 332 (35.4%) patients in the entire cohort. Patients with < 80% compliance had significantly higher overall, minor and surgery-specific complications, longer postoperative stay, delayed functional GI recovery for both Open and MIS procedures. RIOT was observed in 96.5% patients. Duration to RIOT significantly was shorter following Open surgery with ≥ 80% compliance. Poor adherence to ERP was identified as one of the independent predictors for developing postoperative complications.
Conclusion
The study demonstrates beneficial impact on postoperative outcomes with increased adherence to ERP following open and minimally invasive surgery for colorectal cancer. Within a resource limited setting, ERP was found to feasible, safe, and effective in both open and minimally invasive colorectal cancer surgery.
Study of markovian queueing model with F-policy, impatience customers, feedback and re-service, server vacation and breakdown
Authors: Mahla Nitin , Sharma Dinesh C. 
Published: November 2022

Abstract
A system with two heterogeneous servers with impatient customers and finite capacity is considered. The admission control of customers is based on F-policy. This paper is based on M/M/2 queues, and presents the study of two heterogeneous servers where one server is the primary server and other is secondary. Customers are primarily served by the main server, who also takes customer's feedback into account. If customers are unsatisfied and opt for further service, the second server will serve them. The main server considered is unreliable, that is, it may breakdown at any time. The main server will go for vacation whenever there is no customer to serve. To analyze the performance of the model, we calculated the measures of performance and did the cost analysis. Impact of F-policy on system's estimated profit is shown. At last, conclusions are drawn.