bluuebunny/crossref_metadata_2025_split huggingface dataset:
Our Indians in Our America: Anti-Imperialist Imperialism and the Construction of Brazilian Modernity
Authors: Guzmán Tracy Devine 
Published: September 2022

AbstractIndigenous peoples have been used and imagined as guardians of the Brazilian frontier since at least the mid-nineteenth century. This association was central to the foundation of the Indian Protection Service (Serviço de Proteção aos Índios, or SPI) during the early 1900s and culminated with the Amazonian Vigilance System (Sistema de Vigelância da Amazônia, or SIVAM) at the turn of the millennium. Throughout the period, the abiding desire to establish defensive dominion over disputed national territory subjected individuals and groups identified as “Indians” to the power of overlapping discourses of scientific progress, national security, and economic development. A trinity of Brazilian modernity, these goals interpellated native peoples primarily through the practice and rhetoric of education, which grounds their historical relationship with dominant national society. Drawing on SPI records, government documents, journalism, personal testimonies, and visual media, this article traces the impact of this modernist trinity on indigenist policy and in the lives of those who have been affected by its tutelary power. By transforming private indigenous spaces into public domain, Brazil's politics of anti-imperialist imperialism propagated a colonialist, metonymic relationship between “our Indians” and “our America” into the twenty-first century.
Reconstructing Indigenous Ethnicities: The Arapium and Jaraqui Peoples of the Lower Amazon, Brazil
Authors: Bolaños Omaira 
Published: September 2022

AbstractIn Latin America, indigenous identity claims among people not previously recognized as such by the state have become a key topic of anthropological and sociological research. Scholars have analyzed the motivations and political implications of this trend and the impacts of indigenous population's growth on national demographic indicators. However, little is known about how people claiming indigenous status constructs the meaning of their indigenous ethnicity. Drawing from sixty-four in-depth interviews, focus-group analyses, and participant observation, this article explores the double process of identity construction: the reconstruction of the Arapium indigenous identity and the creation of the Jaraqui indigenous identity in Brazil's Lower Amazon. The findings reveal six themes that contribute to the embodiment of a definition of indigenous identity and the establishment of a discursive basis to claim recognition: sense of rootedness, historical memory, historical transformation, consciousness, social exclusion, and identity politics.
‘I'm a boy, can't you see that?’: Dialogic embodiment and the construction of agency in trans youth discourse
Authors: Jones Lucy 
Published: September 2022

AbstractThis article offers discourse analysis of young transgender people's interaction, in which they describe being rendered powerless through misgendering or misrepresentation. It argues that the young people's collective responses to these moments enable them to challenge the ideologies underpinning their marginalisation, and to recontextualise the language used by others to describe their bodies. Stance-taking, the production of affect, and constructed dialogue are shown to be key tools in their production of an agentive, mutual identity. The article thus provides close analysis of dialogic embodiment, a process by which the body is quite literally spoken into being. By critiquing the cisnormative structures which inform and enable the young people's marginalisation, the article responds to the call for a trans linguistics (Zimman 2020) and reflects upon the author's positionality as a cisgender researcher. (Embodiment, affective stance, agency, trans identity, cisnormativity, trans linguistics)*
‘We get that’: Narrative indexicality and the construction of frustration in police stories about domestic violence victim/survivors
Authors: Andrus Jennifer , Clawson Nicole 
Published: September 2022

AbstractThis article considers a particular set of cultural and ideological discourses—police discourse about domestic violence (DV) victim/survivors—in a study about indexicality. Via the processes of indexicality, victim/survivors are consistently described and constructed as frustrations for police officers and police work. We pinpoint two sociosemantic structures that index frustration—the use of the word frustration and statements that initially show understanding for the victim/survivors’ situation—and then mitigate that understanding with stories about being frustrated. In the process, we argue, DV victim/survivors become indexical forms that index the social meaning that victim/survivors are frustrating rather than compliant. Further, we show how such constructions are available for reiteration by different speakers in police discourse and different contexts. The linguistic features that signal ‘frustration’ thus function in police discourse as indexical features that can be accessed and animated by police officers when they describe encounters with victim/survivors and the victim/survivors themselves. (Indexicality, narrative, police discourse, domestic violence)*
Why we need future generations: a defence of direct intergenerational reciprocity
Authors: Corvino Fausto 
Published: September 2022

AbstractIn this article I argue that the non-reciprocity problem does not apply to intergenerational justice. Future generations impact, here and now, on the well-being of people now living. I firstly illustrate the economic-synchronic model of direct intergenerational reciprocity (DIR): future generations allow people now living to maintain the economic system future-oriented and capital-preserving. The rational choice for people now living is to guarantee transgenerational sufficiency to future generations. I then analyse the axiological-synchronic model of DIR: future generations give meaning and value to many of the activities that people now living carry out, and this is a compelling reason for the latter to worry about the former. I argue that only the economic-synchronic model of DIR can consistently explain why we need future generations. I conclude by discussing the limits of indirect intergenerational reciprocity.
Monitoring the 2021 Mw 8.2 Alaska Earthquake by an Offshore Seismic and Fluid Pressure Observation Network and Implications for Ocean‐Crust Dynamic Coupling
Authors: Sun Tianhaozhe , Davis Earl 
Published: September 2022

AbstractGround shaking caused by earthquakes is accompanied by seafloor and sub‐seafloor formation fluid pressure variations in offshore areas, but there have been few collocated observations of these signals. In this work, we report seismic and high‐sampling‐rate fluid pressure records of the 2021 Mw 8.2 Alaska earthquake by the Ocean Networks Canada (ONC) NEPTUNE observatory at an epicentral distance of ∼2,200 km in the northeast Pacific Ocean. The system comprises observatory nodes in various tectonic environments, with each node including buried broadband seismometers, seafloor pressure sensors, and, at two nodes, borehole pressure sensors. Seismic and tsunami waveforms of the Mw 8.2 earthquake were documented in detail. Seismic seafloor pressure variations (Psf) were dominated by Rayleigh waves of periods between 5 and 50 s, with peak amplitudes of 3–4 kPa at most sites. Waveform similarity and the linear scaling between Psf and vertical ground acceleration indicate forced acceleration of the water column being dominant in governing Psf during long‐period surface‐wave arrivals, with an additional component of elastic oscillation occurring at higher frequencies (>0.1 Hz) causing extra pressure signals. Analysis of formation pressure variations due to various types of ocean loading of distinctly different frequencies (e.g., tides, tsunami, and infragravity waves) shows stable one‐dimensional vertical loading efficiencies that depend on lithology at each borehole site, with loading response being strongly influenced by the presence of free gas at shallow depths within the Cascadia accretionary prism. Inter‐site comparisons of seismic and seafloor pressure waveforms demonstrate a key role of sediment thickness in the amplification of surface wave amplitudes.
Bridging Gaps in the Climate Observation Network: A Physics‐Based Nonlinear Dynamical Interpolation of Lagrangian Ice Floe Measurements via Data‐Driven Stochastic Models
Authors: Covington Jeffrey , Chen Nan , Wilhelmus Monica M. 
Published: September 2022

AbstractModeling and understanding sea ice dynamics in marginal ice zones rely on measurements of sea ice. Lagrangian observations of ice floes provide insight into the dynamics of sea ice, the ocean, and the atmosphere. However, optical satellite images are susceptible to atmospheric noise, leading to gaps in the retrieved time series of floe positions. This paper presents an efficient and statistically accurate nonlinear dynamical interpolation framework for recovering missing floe observations. It exploits a balanced physics‐based and data‐driven construction to address the challenges posed by the high‐dimensional and nonlinear nature of the coupled atmosphere‐ice‐ocean system, where effective reduced‐order stochastic models, nonlinear data assimilation, and simultaneous parameter estimation are systematically integrated. The new method succeeds in recovering the locations, curvatures, angular displacements, and the associated strong non‐Gaussian distributions of the missing floes in the Beaufort Sea. It also accurately estimates floe thickness and recovers the unobserved underlying ocean field with an appropriate uncertainty quantification, advancing our understanding of Arctic climate.
DNA2 mutation causing multisystemic disorder with impaired mitochondrial DNA maintenance
Authors: Sun Jiayu , Su Wenwen , Deng Jianwen , Qin Yao , Wang Zhaoxia , Liu Yuhe 
Published: September 2022

Abstract
Purpose
To describe a novel DNA2 variant contributing to defects in mtDNA maintenance and mtDNA depletion syndrome (MDS), and the clinical and histological findings associated with this variation.

Methods
Herein, we describe the case of a patient who presented with hearing loss and myopathy, given the family history of similar findings in the father, was evaluated by sequencing of the deafness gene panel, mitochondrial genome, and the exome. Furthermore, tissue staining, mtDNA copy number detection, mtDNA sequencing, and long-range polymerase chain reaction tests were also conducted on the muscle biopsy specimen. In vitro experiments, including analyses of the mtDNA copy number; levels of ATP, ATPase, and reactive oxygen species (ROS); and the membrane potential, were performed.

Results
The DNA2 heterozygous truncating variant c. 2368C > T (p.Q790X) was identified and verified as the cause of an mtDNA copy number decrement in both functional experiments and muscle tissue analyses. These changes were accompanied by reductions in ATP, ATPase, and ROS levels.

Conclusion
The DNA2 variant was a likely cause of MDS in this patient. These findings expand the mutational spectrum of MDS and improve our understanding of the functions of DNA2 by revealing its novel role in mtDNA maintenance.
Mesenchymal stromal cells alleviate acute respiratory distress syndrome through the cholinergic anti-inflammatory pathway
Authors: Zhang Xiaoran , Wei Xuxia , Deng Yiwen , Yuan Xiaofeng , Shi Jiahao , Huang Weijun , Huang Jing , Chen Xiaoyong , Zheng Shuwei , Chen Jieying , Chen Keyu , Xu Ruiming , Wang Hongmiao , Li Weiqiang , Li Shiyue , Yi Huimin , Xiang Andy Peng 
Published: September 2022

AbstractMesenchymal stromal cells (MSCs) have been considered a promising alternative for treatment of acute respiratory distress syndrome (ARDS). However, there is significant heterogeneity in their therapeutic efficacy, largely owing to the incomplete understanding of the mechanisms underlying the therapeutic activities of MSCs. Here, we hypothesize that the cholinergic anti-inflammatory pathway (CAP), which is recognized as a neuroimmunological pathway, may be involved in the therapeutic mechanisms by which MSCs mitigate ARDS. Using lipopolysaccharide (LPS) and bacterial lung inflammation models, we found that inflammatory cell infiltration and Evans blue leakage were reduced and that the expression levels of choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in lung tissue were significantly increased 6 hours after MSC infusion. When the vagus nerve was blocked or α7 nicotinic acetylcholine (ACh) receptor (α7nAChR)-knockout mice were used, the therapeutic effects of MSCs were significantly reduced, suggesting that the CAP may play an important role in the effects of MSCs in ARDS treatment. Our results further showed that MSC-derived prostaglandin E2 (PGE2) likely promoted ACh synthesis and release. Additionally, based on the efficacy of nAChR and α7nAChR agonists, we found that lobeline, the nicotinic cholinergic receptor excitation stimulant, may attenuate pulmonary inflammation and alleviate respiratory symptoms of ARDS patients in a clinical study (ChiCTR2100047403). In summary, we reveal a previously unrecognized MSC-mediated mechanism of CAP activation as the means by which MSCs alleviate ARDS-like syndrome, providing insight into the clinical translation of MSCs or CAP-related strategies for the treatment of patients with ARDS.
MTG8 interacts with LHX6 to specify cortical interneuron subtype identity
Authors: Asgarian Zeinab , Oliveira Marcio Guiomar , Stryjewska Agata , Maragkos Ioannis , Rubin Anna Noren , Magno Lorenza , Pachnis Vassilis , Ghorbani Mohammadmersad , Hiebert Scott Wayne , Denaxa Myrto , Kessaris Nicoletta 
Published: September 2022

AbstractCortical interneurons originating in the embryonic medial ganglionic eminence (MGE) diverge into a range of different subtypes found in the adult mouse cerebral cortex. The mechanisms underlying this divergence and the timing when subtype identity is set up remain unclear. We identify the highly conserved transcriptional co-factor MTG8 as being pivotal in the development of a large subset of MGE cortical interneurons that co-expresses Somatostatin (SST) and Neuropeptide Y (NPY). MTG8 interacts with the pan-MGE transcription factor LHX6 and together the two factors are sufficient to promote expression of critical cortical interneuron subtype identity genes. The SST-NPY cortical interneuron fate is initiated early, well before interneurons migrate into the cortex, demonstrating an early onset specification program. Our findings suggest that transcriptional co-factors and modifiers of generic lineage specification programs may hold the key to the emergence of cortical interneuron heterogeneity from the embryonic telencephalic germinal zones.
Discovery of an orally active benzoxaborole prodrug effective in the treatment of Chagas disease in non-human primates
Authors: Padilla Angel M. , Wang Wei , Akama Tsutomu , Carter David S. , Easom Eric , Freund Yvonne , Halladay Jason S. , Liu Yang , Hamer Sarah A. , Hodo Carolyn L. , Wilkerson Gregory K. , Orr Dylan , White Brooke , George Arlene , Shen Huifeng , Jin Yiru , Wang Michael Zhuo , Tse Susanna , Jacobs Robert T. , Tarleton Rick L. 
Published: September 2022

AbstractTrypanosoma cruzi, the agent of Chagas disease, probably infects tens of millions of people, primarily in Latin America, causing morbidity and mortality. The options for treatment and prevention of Chagas disease are limited and underutilized. Here we describe the discovery of a series of benzoxaborole compounds with nanomolar activity against extra- and intracellular stages of T. cruzi. Leveraging both ongoing drug discovery efforts in related kinetoplastids, and the exceptional models for rapid drug screening and optimization in T. cruzi, we have identified the prodrug AN15368 that is activated by parasite carboxypeptidases to yield a compound that targets the messenger RNA processing pathway in T. cruzi. AN15368 was found to be active in vitro and in vivo against a range of genetically distinct T. cruzi lineages and was uniformly curative in non-human primates (NHPs) with long-term naturally acquired infections. Treatment in NHPs also revealed no detectable acute toxicity or long-term health or reproductive impact. Thus, AN15368 is an extensively validated and apparently safe, clinically ready candidate with promising potential for prevention and treatment of Chagas disease.
Multiproxy study of 7500-year-old wooden sickles from the Lakeshore Village of La Marmotta, Italy
Authors: Mazzucco Niccolò , Mineo Mario , Arobba Daniele , Caramiello Rosanna , Caruso Fermé Laura , Gassin Bernard , Guilbeau Denis , Ibáñez Juan José , Morandi Lionello F. , Mozota Millán , Pichon Fiona , Portillo Marta , Rageot Maxime , Remolins Gerard , Rottoli Mauro , Gibaja Juan F. 
Published: September 2022

AbstractThe lakeshore site of La Marmotta is one of the most important Early Neolithic sites of Mediterranean Europe. The site is famous for the exceptional preservation of organic materials, including numerous wooden artefacts related to navigation, agriculture, textile production, and basketry. This article presents interdisciplinary research on three of the most complete and well-preserved sickles recovered from the site, yet unpublished. All the components of the tools are analysed: the stone inserts, the wooden haft and the adhesive substances used to fix the stones inside the haft. Our innovative methodology combines use-wear and microtexture analysis of stone tools through confocal microscopy, taxonomical and technological analysis of wood, gas chromatography–mass spectrometry analysis of the adhesive substances, and pollen, non-pollen palynomorphs, and phytolith analysis of the remains incorporated within the adhesive. This multiproxy approach provides a significant insight into the life of these tools, from their production to their use and abandonment, providing evidence of the species of harvested plants and the conditions of the field during the harvesting.
Metatranscriptome analysis in human papillomavirus negative cervical cancers
Authors: Ure Agustin Enrique , Lagheden Camilla , Arroyo Mühr Laila Sara 
Published: September 2022

AbstractHuman papillomavirus (HPV) negative cancers are associated with symptomatic detection, late-stage diagnosis, and worse prognosis. It is thus essential to investigate all possible infectious agents and biomarkers that could early identify these HPV negative cancers. We aimed to analyze and compare the metatranscriptome present in HPV positive and HPV negative cervical cancers. We analyzed the whole RNA sequencing files from 223 HPV negative cervical cancers (negativity established after confirming cervical cancer diagnosis, sample adequacy and subjecting specimens to PCR and unbiased RNA sequencing), 223 HPV positive tumors and 11 blank paraffin block pools (used as controls) using Kraken2 software. Overall, 84 bacterial genera were detected, with 6/84 genera showing a positive median number of reads/sample and being present in both cervical tumor groups (HPV positive and negative). Viral reads belonged to 63 different viral genera, with 6/63 genera showing a positive median annotated read/sample value. No significant difference among genera was detected except for the presence of alpha-papillomaviruses. Metatranscriptome of bacteria and viruses present in HPV positive and HPV negative cervical cancers show no significant difference, except for HPV. Further studies are needed to early identify this biologically distinct group of cervical cancers.
Combining donor-derived cell-free DNA and donor specific antibody testing as non-invasive biomarkers for rejection in kidney transplantation
Authors: Obrișcă Bogdan , Butiu Maria , Sibulesky Lena , Bakthavatsalam Ramasamy , Smith Kelly D. , Gimferrer Idoia , Warner Paul , Ismail Gener , Leca Nicolae 
Published: September 2022

AbstractDonor specific anti-HLA antibodies (DSA) and donor-derived cell-free DNA (dd-cfDNA) have lead to substantial progress in the non-invasive monitoring of the renal allograft by being able to detect or rule out subclinical rejection and guide immunosuppressive changes. In this study we sought to analyze the clinical, de novo DSA (dnDSA) and histological determinants of dd-cfDNA levels. The study included a cohort of stable renal function kidney transplant (KT) recipients who underwent anti-HLA dnDSA and dd-cfDNA testing between September 2017-December 2019. Statistical models were constructed to detect association with predictors of dd-cfDNA levels and other clinical characteristics. 171 renal allograft recipients were tested for dd-cfDNA and dnDSA at a median 1.06 years posttransplant (IQR: 0.37–4.63). Median dd-cfDNA was 0.25% (IQR: 0.19–0.51), 18.7% of patients having a dd-cfDNA ≥ 1%. In a multivariate linear regression model the presence of dnDSA MFI ≥ 2500 was the best independent determinant of dd-cfDNA level (p < 0.001). Among patients tested, 54 had concurrent dd-cfDNA determination at the time of an allograft biopsy. dd-cfDNA had an AUC of 0.82 (95% CI 0.69–0.91; p < 0.001) and of 0.96 (95% CI 0.87–0.99) to discriminate any rejection and ABMR, respectively. After multivariate adjustment, the models that included ABMR (R = 0.82, R2 = 0.67, p < 0.001), or ptc (R = 0.79, R2 = 0.63, p < 0.001) showed the best correlation with dd-cfDNA level. We are confirming a strong association of dd-cfDNA with dnDSA and underlying alloimmune-mediated injury in renal allograft recipients in a cohort of patients with unsuspecting clinical characteristics for rejection and excellent allograft function. Our findings support the need for noninvasive biomarker surveillance in KT recipients and we propose that dd-cfDNA may complement dnDSA screening.
Stability and volatility shape the gut bacteriome and Kazachstania slooffiae dynamics in preweaning, nursery and adult pigs
Authors: Feehan Brandi , Ran Qinghong , Dorman Victoria , Rumback Kourtney , Pogranichniy Sophia , Ward Kaitlyn , Goodband Robert , Niederwerder Megan C. , Summers Katie Lynn , Lee Sonny T. M. 
Published: September 2022

AbstractThe gut microbiome plays important roles in the maintenance of health and pathogenesis of diseases in the growing host. In order to fully comprehend the interplay of the gut microbiome and host, a foundational understanding of longitudinal microbiome, including bacteria and fungi, development is necessary. In this study, we evaluated enteric microbiome and host dynamics throughout the lifetime of commercial swine. We collected a total of 234 fecal samples from ten pigs across 31 time points in three developmental stages (5 preweaning, 15 nursery, and 11 growth adult). We then performed 16S rRNA gene amplicon sequencing for bacterial profiles and qPCR for the fungusKazachstania slooffiae. We identified distinct bacteriome clustering according to the host developmental stage, with the preweaning stage exhibiting low bacterial diversity and high volatility amongst samples. We further identified clusters of bacteria that were considered core, increasing, decreasing or stage-associated throughout the host lifetime.Kazachstania slooffiaewas absent in the preweaning stage but peaked during the nursery stage of the host. We determined that all host growth stages contained negative correlations betweenK. slooffiaeand bacterial genera, with only the growth adult stage containing positive correlates. Our stage-associated bacteriome results suggested the neonate contained a volatile gut microbiome. Upon weaning, the microbiome became relatively established with comparatively fewer perturbations in microbiome composition. Differential analysis indicated bacteria might play distinct stage-associated roles in metabolism and pathogenesis. The lack of positive correlates and sharedK. slooffiae-bacteria interactions between stages warranted future research into the interactions amongst these kingdoms for host health. This research is foundational for understanding how bacteria and fungi develop singularly, as well as within a complex ecosystem in the host’s gut environment.