bluuebunny/crossref_metadata_2025_split huggingface dataset:
Facilitators and barriers of women's participation in HIV clinical research in Switzerland: A qualitative study
Authors: Courvoisier Nelly , Storari Chiara , Lesage Saphir , Vittoz Lucie , Barbieux Charlotte , Peytremann‐Bridevaux Isabelle , Gilles Ingrid , Calmy Alexandra 
Published: February 2022

AbstractObjectivesWomen are underrepresented in most HIV clinical trials in Western countries, but their participation remains crucial as the lack of information on sex‐ and gender‐specific effects may hinder the safety and efficacy of antiretroviral treatments. The aim of this study was to identify barriers to and facilitators of women's participation in HIV clinical trials in Switzerland.MethodsWe conducted semi‐structured interviews among 20 women with HIV to explore factors associated with non‐participation in clinical trials. The interviewer presented to participants a clinical trial's description and discussed it with them. Lexicometric analysis on transcribed interviews identified three themes and eight sub‐themes related to the pros and cons of participation in HIV clinical trials.ResultsParticipants evoked mainly decision‐making drivers, concerns for women living with HIV and treatment side‐effects. They highlighted the need for extensive information provided by trusted healthcare professionals on the research process as central to the decision to enrol in HIV clinical trials. Familial responsibilities were clearly identified as barriers to their participation, but not pregnancy. Additional preoccupations were other health concerns and comorbidities and the consequences of stopping ongoing antiretroviral treatments.ConclusionsTo overcome the barriers to the participation of women living with HIV in clinical research in Western countries, healthcare professionals and researchers should increase women's research literacy by involving them in the study design and by tailoring clinical trials to their social roles and health concerns. Trust in professionals is a facilitator of enrolment of women living with HIV that should be maintained.
Synergetic effect of petit‐high pressure carbon dioxide combined with cinnamon (Cinnamomum cassia) essential oil against Salmonella typhimurium
Authors: Niu Liyuan , Zhang Yilin , Jie Mingsha , Cheng Yingxin , Xiang Qisen , Zhang Zhijian , Bai Yanhong 
Published: February 2022

SummaryThis work aimed to evaluate the synergistic effect of petit‐high pressure carbon dioxide (p‐HPCD) and cinnamon (Cinnamomum cassia) essential oil (CEO) against Salmonella typhimurium. The results showed synergetic antibacterial activity of p‐HPCD and CEO. After treatment with p‐HPCD and CEO with a final concentration of 0.03125% under 1.3 MPa at 30 °C for 5 h, the population of S. typhimurium was decreased by 6.22 log10 CFU/mL, significantly higher than p‐HPCD or CEO alone. The combined treatment induced severe cellular changes in S. typhimurium, including cell membrane disruption, decreased intracellular protein and ATP, and intracellular reactive oxygen species (ROS) generation. Moreover, p‐HPCD + CEO treatment significantly inhibited the expression of virulence genes including fliC, hilA, invI and ssrA, indicating that p‐HPCD + CEO can reduce the production of virulence factors in S. typhimurium. This study provides a new approach to the application of p‐HPCD and CEO in food processing and preservation.
The combination of thymol and cinnamaldehyde reduces the survival and virulence of Listeria monocytogenes on autoclaved chicken breast
Authors: Liang Siwei , Hu Xinyi , Wang Ruifei , Fang Meimei , Yu Yigang , Xiao Xinglong 
Published: February 2022

Abstract

Aims
To reveal the antibacterial mechanism of the combination of thymol and cinnamaldehyde to Listeria monocytogenes ATCC 19115 on autoclaved chicken breast.

Methods and Results
In this study, L. monocytogenes ATCC 19115 on autoclaved chicken breast was exposed to the stress of 125 μg/ml thymol and 125 μg/ml cinnamaldehyde, and transcriptome analysis was used to reveal the crucial antibacterial mechanism. According to the results, 1303 significantly differentially expressed genes (DEGs) were identified. Treated by thymol and cinnamaldehyde in combination, pyrimidine and branched-chain amino acid biosynthesis of L. monocytogenes were thwarted which impairs its nucleic acid biosynthesis and intracellular metabolism. The up-regulated DEGs involved in membrane composition and function contributed to membrane repair. Besides, pyruvate catabolism and TCA cycle were restrained which brought about the disturbance of amino acid metabolism. ABC transporters were also perturbed, for instance, the uptake of cysteine, D-methionine, and betaine was activated, while the uptake of vitamin, iron, and carnitine was repressed. Thus, L. monocytogenes tended to activate PTS, glycolysis, glycerol catabolism, and pentose phosphate pathways to obtain energy to adapt to the hostile condition. Noticeably, DEGs involved in virulence factors were totally down-regulated, including genes devoted to encoding flagella, chemotaxis, biofilm formation, internalin as well as virulence gene clusters.

Conclusions
The combination of thymol and cinnamaldehyde is effective to reduce the survival and potential virulence of L. monocytogenes on autoclaved chicken breast.

Significance and Impact of Study
This work contributes to providing theoretical information for the application and optimization of thymol and cinnamaldehyde in ready-to-eat meat products to inhibit L. monocytogenes.
Potential hazards associated with the consumption of Scombridae fish: Infection and toxicity from raw material and processing
Authors: Yemmen Chiraz , Gargouri Mohamed 
Published: February 2022

Abstract
Scombridae fish (tuna, bonito and mackerel) have significant ecological and economic values. They are very appreciated by consumers worldwide for their high-quality flesh and for their high nutritional value. However, consumption of Scombridae fish is potentially hazardous. Indeed, several cases of infections and toxicity linked to the consumption of Scombridae fish as raw, or processed food products have been reported worldwide. In this review, we presented the most common health risks associated with Scombridae fish consumption. Diseases associated with the consumption of these fish are generally infectious or toxic and are caused by biological hazards, such as bacteria, viruses, parasites or chemicals hazards that enter the body through contaminated fish (polycyclic aromatic hydrocarbons, histamine) or by physical contaminants, such as heavy metals. The risks of contamination exist throughout the food chain, from primary production to the preparation of products for consumption.
Synovial tissue‐derived extracellular vesicles induce chondrocyte inflammation and degradation via NF‐κB signalling pathway: An in vitro study
Authors: Chen Pu , Zhou Jun , Ruan Anmin , Guan Hua , Xie Jiewei , Zeng Lingfeng , Liu Jun , Wang Qingfu 
Published: February 2022

AbstractOsteoarthritis (OA) is a whole‐joint disease characterized by synovial inflammation and cartilage degeneration. However, the relationship between synovial inflammation and cartilage degeneration remains unclear. The modified Hulth's method was adopted to establish a knee OA (KOA) rabbit model. Synovial tissue was collected after 8 weeks, and synovial tissue‐derived extracellular vesicles (ST‐EVs) were extracted by filtration combined with size exclusion chromatography (SECF), followed by identification through transmission electron microscopy (TEM), nanoparticle tracer analysis (NTA) and Western blot (WB). The collagenase digestion method was used to extract normal rabbit chondrocytes, which were then treated with the SF‐EVs to observe the effect and mechanism of SF‐EVs on chondrocytes. The morphology, particle size and labelled protein marker detection confirmed that SECF successfully extract ST‐EVs. The ST‐EVs in the KOA state significantly inhibited chondrocyte proliferation and promoted chondrocytes apoptosis. Moreover, the ST‐EVs also promoted the expression of pro‐inflammatory cytokines (IL‐1β, IL‐6, TNF‐α and COX‐2) and cartilage degradation‐related enzymes (MMP13, MMP9 and ADAMTS5) in the chondrocytes. Mechanistically, the ST‐EVs significantly promoted the activation of NF‐κB signalling pathway in chondrocytes. Inhibition the activation of the NF‐κB signalling pathway significantly rescued the expression of inflammatory cytokines and cartilage degradation‐related enzymes in the ST‐EVs–induced chondrocytes. In conclusion, the ST‐EVs promote chondrocytes inflammation and degradation by activating the NF‐κB signalling pathway, providing novel insights into the occurrence and development of OA.
Radiation‐induced C‐reactive protein triggers apoptosis of vascular smooth muscle cells through ROS interfering with the STAT3/Ref‐1 complex
Authors: Ryu Je‐Won , Jung In‐Hye , Park Eun‐Young , Kim Kang‐Hyun , Kim Kyunggon , Yeom Jeonghun , Jung Jinhong , Lee Sang‐wook 
Published: February 2022

AbstractDamage to normal tissue can occur over a long period after cancer radiotherapy. Free radical by radiation can initiate or accelerate chronic inflammation, which can lead to atherosclerosis. However, the underlying mechanisms remain unclear. Vascular smooth muscle cells (VSMCs) proliferate in response to JAK/STAT3 signalling. C‐reactive protein (CRP) can induce VSMCs apoptosis via triggering NADPH oxidase (NOX). Apoptotic VSMCs promote instability and inflammation of atherosclerotic lesions. Herein, we identified a VSMCs that switched from proliferation to apoptosis through was enhanced by radiation‐induced CRP. NOX inhibition using lentiviral sh‐p22phox prevented apoptosis upon radiation‐induced CRP. CRP overexpression reduced the amount of STAT3/Ref‐1 complex, decreased JAK/STAT phosphorylation and formed a new complex of Ref‐1/CRP in VSMC. Apoptosis of VSMCs was further increased by CRP co‐overexpressed with Ref‐1. Functional inhibition of NOX or p53 also prevented apoptotic activity of the CRP‐Ref‐1 complex. Immunofluorescence showed co‐localization of CRP, Ref‐1 and p53 with α‐actin‐positive VSMC in human atherosclerotic plaques. In conclusion, radiation‐induced CRP increased the VSMCs apoptosis through Ref‐1, which dissociated the STAT3/Ref‐1 complex, interfered with JAK/STAT3 activity, and interacted with CRP‐Ref‐1, thus resulting in transcription‐independent cell death via p53. Targeting CRP as a vascular side effect of radiotherapy could be exploited to improve curability.
Biotyping reveals loss of motility in two distinct Yersinia ruckeri lineages exclusive to Norwegian aquaculture
Authors: Riborg Andreas , Colquhoun Duncan J. , Gulla Snorre 
Published: February 2022

AbstractNon‐motile strains of Yersinia ruckeri, known as Y. ruckeri biotype 2, now dominate amongst clinical isolates retrieved from rainbow trout internationally. Due to an acute increase in the number of yersiniosis cases in Norway in recent years, followed by introduction of widespread intraperitoneal vaccination against the disease, an investigation on the prevalence of Y. ruckeri biotype 2 in Norwegian aquaculture was conducted. We biotyped 263 Y. ruckeri isolates recovered from diseased salmonids in Norway between 1985 and 2020. A total of seven biotype 2 isolates were identified, four of which were collected between 1985 and 1987, and three of which belong to the current epizootic clone, isolated from two different sea‐farms in 2017. Whole‐genome sequencing revealed single non‐synonymous nucleotide polymorphisms in the flagellar genes flhC in isolates from the 1980s, and in fliP in isolates from 2017. In both variants, motility was restored both by complementation with wild‐type alleles in trans and via spontaneous mutation‐driven reversion following prolonged incubation on motility agar. While biotype 2 strains do not yet seem to have become broadly established in Norwegian aquaculture, the seven isolates described here serve to document a further two independent cases of Y. ruckeri biotype 2 emergence in salmonid aquaculture.
Recognizing strawberry appearance quality using different combinations of deep feature and classifiers
Authors: Li Xuchen , Cai Changlong , Zheng Hao , Zhu Hongfei 
Published: February 2022

AbstractThe quality of the strawberry appearance is the most important indicator for consumers and modern fruit process engineering, which is closely associated with the ripeness and diseases. The continuous development of profound deep learning has greatly helped the recognition of strawberry appearance quality, granting a vigorous tool of relative precise outcomes, but the better performance of deep learning requires more time and more computation for training. This paper analysis the efficiency of different combination models that utilize deep feature plus classifiers for recognizing strawberry. The six convolutional neural networks are used to extract the deep feature and then the feature is imported into eight classifiers for classification. The average accuracy of six combination models is 1.53% higher than transfer learning. Besides, a method combining ResNet101 plus linear discriminant analysis (LDA) is proposed. The results show that the accuracy of the proposed method is 96.55%, which is superior to the transfer learning. Moreover, the training time of the method is 57.70 s faster than transfer learning. Therefore, this method has positive significance for the development of recognizing strawberry appearance quality.Practical ApplicationsThe goal of our study is to make it easier for farmers to accurately classify the appearance of strawberries in real situations. This paper analysis the efficiency of different combination models that utilize deep feature plus classifiers for recognizing strawberry. Meanwhile, we have proposed a method combining ResNet101 with linear discriminant analysis (LDA), which obtain the classification task more accurate and efficient. This method has an important role in recognizing strawberry, which will facilitate the development of efficient sorting equipment and provide a viable strategy for future smart sorting methods. Therefore, this work has practical application value.
Potential food safety hazards in fermented and salted fish in Egypt (Feseekh, Renga, Moloha) as case studies and controlling their manufacture using HACCP system
Authors: Farag Mohamed A. , Zain Aya Ehab , Hariri Mohamad Louai , el Aaasar Reem , Khalifa Ibrahim , Elmetwally Farah 
Published: February 2022

AbstractThe consumption of raw fish has significantly increased worldwide over the last decade. Fermented and salted fish (FSF), such as feseekh, moloha, and renga, have a great popularity in Egypt, where they are considered the main dishes in different social events and festivities. Although FSF‐dishes are popular, they present several health hazards including biological and chemical ones. These hazards are contaminants that might be present in the fish tissues and to be transmitted to humans causing food‐borne illnesses, that is, botulism intoxication. For the sake of public health, methods of detecting and controlling these contaminants have been employed to regulate and control fish's safety and quality prior to consumption to ensure their safety and best quality. We highlighted the hazards, detection methods, critical control points, and control measures involved in the production of main Egyptian FSF. In this review, we also compile the research findings from the available literatures, with future perspectives related to the quality needed for producing a safe FSF. Our review concluded that hazard analysis critical control point (HACCP) system could also be utilized as a preventive measure likewise to guarantee FSF's safety to consumers. Future experiments are needed to determine the underlying mechanisms of microbial impact on the quality of FSF and the optimization of the fermentation process.
ENETS standardized (synoptic) reporting for endoscopy in neuroendocrine tumors
Authors: Borbath Ivan , Pape Ulrich‐Frank , Deprez Pierre H. , Bartsch Detlef Klaus , Caplin Martyn , Falconi Massimo , Garcia‐Carbonero Rocio , Grozinsky‐Glasberg Simona , Jensen Robert T. , Arnold Rudolf , Ruszniewski Philippe , Toumpanakis C. , Valle Juan W. , O´Toole Dermot ,   for the Members of the Advisory Board of the European Neuroendocrine Tumor Society (ENETS)
Published: February 2022

AbstractDespite efforts from various endoscopy societies, reporting in the field of endoscopy remains extremely heterogeneous. Harmonisation of clinical practice in endoscopy has been highlighted by application of many clinical practice guidelines and standards pertaining to the endoscopic procedures and reporting are underlined. The aim of the proposed “standardised reporting” is to (1) facilitate recognition of gastrointestinal neuroendocrine neoplasms (NEN) on initial endoscopy, (2) to enable interdisciplinary decision making for treatment by a multidisciplinary team, (3) to provide a basis for a standardised endoscopic follow‐up which allows detection of recurrence or progression reliably, (4) to make endoscopic reports on NEN comparable between different units, and (5) to allow research collaboration between NEN centres in terms of consistency of their endoscopic data. The ultimate goal is to improve disease management, patient outcome and reduce the diagnostic burden on the side of the patient by ensuring the highest possible diagnostic accuracy and validity of endoscopic exams and possibly interventions.
Ablative effect of the interstitial laser setting in the human placental model
Authors: Nawapun Katika , Hunsawongsakul Daungporn , Phithakwatchara Nisarat , Hanamornroongruang Suchanan , Viboonchart Sommai , Jaingam Suparat , Wataganara Tuangsit 
Published: February 2022

AbstractAimTo assess the impact of laser power and time on interstitial ablation generated by neodymium‐doped yttrium aluminium garnet (Nd:YAG) and diode laser in the human placental model.MethodsThe experiment was carried out in a simulation model of interstitial laser ablation on ex‐vivo placental tissue. One‐hundred and forty‐four pieces of fresh placentae were interstitially ablated with Nd:YAG or diode laser at various power (15, 20, 25, 30 W)‐time (5, 10, 15 s) combinations. The ablation tissues were evaluated using both sonographic and histopathologic measurements.ResultsLaser generator, power, and time significantly affected the ablation size (p < 0.001). The coagulation zone continuously increased with extending time at the power of 15, 20, and 25 W. When adjusting to the power of 30 W, increased time from 10 to 15 s did not induce the larger coagulation diameter. The maximal diameter was obtained at the laser power of 20 W for 15 s. The ablation from the diode laser was greater than that from Nd:YAG laser. The sonographic evaluation overestimated the ablation size by an average of 24%.ConclusionDiode laser destroys greater tissue than Nd:YAG laser. Different power settings of interstitial laser ablation produce diverse patterns of correlation between laser time and coagulation size.
Association of maternal and umbilical cord blood asprosin with excessive gestational weight gain
Authors: Behrasi Fatemeh , Karajibani Mansour , Khayat Samira , Fanaei Hamed , Montazerifar Farzaneh 
Published: February 2022

AbstractMaternal obesity and excessive gestational weight gain (EGWG) may have persistent effects on offspring obesity and be risk factors for both mother and child. Studies have reported that asprosin concentration is associated with metabolism and weight changes during pregnancy. This study therefore evaluated the association of maternal and newborn asprosin with normal weight gain and EGWG and nutritional intake in pregnancy. The study included 30 pregnant women with normal weight gain and 30 women with EGWG referred to Ali ibn Abi Talib Hospital in Zahedan, Iran, September 2020 to June 2021. A demographic questionnaire and neonatal complications checklist were completed. Asprosin concentrations at the time of delivery in maternal venous blood samples and umbilical vein blood samples were measured by ELISA technique. Macronutrient and energy intake were assessed by a 24‐h dietary recall questionnaire and compared with standard Recommended Dietary Allowance (RDA) and Dietary References Intake (DRI) values. Asprosin levels were significantly higher in the EGWG group's umbilical cord blood than in the control group but there was no difference in maternal asprosin concentrations between the two groups. The Appearance, Pulse, Grimace, Activity and Respiration (Apgar) score was significantly lower in the EGWG group than in the control group, although there was no difference in birthweight between the groups. Weight gain during pregnancy, energy intake and protein and carbohydrate consumption were significantly higher in the EGWG group than in the control group (p < 0.05). It seems that differences in asprosin secretion do not mediate an imbalance in energy intake and other macronutrients during pregnancy that affect pregnancy weight gain and birthweight. However, excessive gestational weight gain was associated with neonatal asprosin levels and newborn Apgar scores.
Conservation and architecture of housekeeping genes in the model marine diatom Thalassiosira pseudonana
Authors: Li Zhengke , Zhang Yong , Li Wei , Irwin Andrew J. , Finkel Zoe V. 
Published: February 2022

Summary

Housekeeping genes (HKGs) are constitutively expressed with low variation across tissues/conditions. They are thought to be highly conserved and fundamental to cellular maintenance, with distinctive genomic features.

Here, we identify 1505 HKGs in the unicellular marine diatom Thalassiosira pseudonana based on an RNA‐seq analysis of 232 samples taken under 12 experimental conditions over 0–72 h. We identify promising internal reference genes (IRGs) for T. pseudonana from the most stably expressed HKGs.

A comparative analysis indicates < 18% of HKGs in T. pseudonana have orthologs in other eukaryotes, including other diatom species. Contrary to work on human tissues, T. pseudonana HKGs are longer than non‐HKGs, due to elongated introns. More ancient HKGs tend to be shorter than more recent HKGs, and expression levels of HKGs decrease more rapidly with gene length relative to non‐HKGs.

Our results indicate that HKGs are highly variable across the tree of life and thus unlikely to be universally fundamental for cellular maintenance. We hypothesize that the distinct genomic features of HKGs of T. pseudonana may be a consequence of selection pressures associated with high expression and low variance across conditions.
CULTURAL HYBRIDIZATION IN THE AEGEAN EARLY BRONZE AGE: THE POTTERY FROM AIGION IN ACHAEA
Authors: Michalopoulos Panagiotis 
Published: February 2022

SummaryAround the middle of the third millennium BC drastic changes occur in the Aegean in several aspects of material life. One such important change is the introduction of Anatolianizing shapes and features into the ceramic traditions of the Cyclades and the southern east‐central Greek Mainland. In this article, I use the ceramic assemblage from the Minasian plot at Aigion to explore these ceramic changes in the southern Greek mainland (Peloponnese) within the framework of cultural hybridization theory. By focusing on the specific example of Aigion, I discuss the multiple factors which contribute to ceramic hybridization, while I explore their impact on our understanding of broader cultural change at Aigion at this time.