bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biochar Combined with Organic and Inorganic Fertilizers Promoted the Rapeseed Growth and Improved the Soil Quality in Purple Soil
Authors: Liu Ming , Linna Cholidah , Ma Shumin , Ma Qun , Song Wenfeng , Shen Mingzhu , Song Lixia , Cui Kaidong , Zhou Yuling , Wang Longchang 
Published: July 2022

Abstract
Background Biochar is one kind of organic matter that can be added into soil as a soil amendment to improve its quality. To study the effect of biochar addition combined with organic and inorganic fertilizers on growth and fertility and microbial community in purple soil, a completely randomized block design was designed with three levels of biochar [B0: no biochar, B1: low-rate biochar (35 t/ha) , B2: high-rate biochar (50 t/ha)]; two levels of inorganic fertilizers [F1: low-rate inorganic fertilizer (30 kg/ha N, 87.5 kg/ha P2O5 and 60 kg/ha K2O); F2: high-rate inorganic fertilizer (60 kg/ha N, 175 kg/ha P2O5 and 120 kg/ha K2O)]; and two levels of organic fertilizers [M1: no organic fertilizer; M2: with organic fertilizer (4.5 t/ha)]. Results With the application of biochar, the plant height and stem diameter of rapeseed were increased by 6.15%-9.80% and 16.37%-20.11%, respectively. The photosynthetic capacity increased by 20.25% to 35.83%, and the yield increased by 16.40% to 19.11%, respectively. It also promoted the absorption and utilization of nitrogen, phosphorus and potassium by rapeseed to varying degrees. At the same time, the combined application of biochar and organic and inorganic fertilizers could improve soil pH, nitrogen, phosphorus and potassium content and soil microbial community richness: The pH of B1F2M1 increased 0.41 compared with the control, the nitrogen, phosphorus and potassium content in the soil increased by 103.95%, 117.88% and 99.05%, respectively. Meanwhile, soil microbial community richness was also improved. Conclusions Our research showed that biochar could promote the growth and development of rapeseed, and the combined application of biochar with organic and inorganic fertilizers could improve soil fertility and increase microbial diversity. Low-rate biochar combined with organic fertilizer and low-rate inorganic fertilizer was the most suitable application mode in rapeseed production in purple soil area of Southwest China.
A new approach for studying the stability and degradation products of ascorbic acid in solutions
Authors: Pilarski Bogusław , Wyrzykowski Dariusz , Młodzianowski Janusz 
Published: July 2022

Abstract
The potentiometric titration (PT) method has been applied for the first time to investigate the stability of L-ascorbic acid (H2Asc) and to determine its degradation products in aqueous solutions. The presented electrochemical procedures can be considered to be a fast, simple and inexpensive way to control the stability of H2Asc in a regular analytical practice as well as in the chemical and pharmaceutical industries. Experimental data as well as modeling suggest that the enolic form of 2,3-diketogulonic acid predominates in a solution as the main product of dehydroascorbate (DHA) degradation. Furthermore, the PT results supported by conductometric measurements (CM) and electrospray ionization mass spectrometry (ESI/MS) data enable us to propose the putative mechanism of the H2Asc decomposition. Moreover, it has been proven that among different types of investigated electrolytes (KNO3, KClO4 and KSCN), the thiocyanate ions (SCN-) reveal the stabilizing effect against the degradation of H2Asc. Thus, the presence of SCN- in the H2Asc solution is proposed as an alternative way for some organic solvents earlier used. Finally, a new paraffin-protection-layer procedure has been recommended for studying as well as storage of the solutions comprising components sensitive to external factors (e.g. O2, CO2) and to evaporation.
A novel NADC34-like porcine reproductive and respiratory syndrome virus 2 with complex genome recombination is highly pathogenic to piglets
Authors: Zhou Long , Yu Jifeng , Zhou Jun , Long Yaoping , Xiao Lu , Fan Yandi , Yang Danjiao , Zhang Bin , Zhang Zhidong , Liu Jie 
Published: July 2022

Abstract
Background: The NADC34-like porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) first emerged in China in 2017 and has the potential to become the dominant PRRSV strain in China. This study aimed to analyze the genetic features and investigate the pathogenicity of a novel NADC34-like PRRSV in southwest China. Results: In this study, a novel PRRSV-2, SCcd2020, was isolated from diseased piglets in Sichuan province, southwest China in 2020. The complete viral genome was determined and analyzed. An ORF5-based phylogenetic analysis showed that SCcd2020 clustered with NADC34-like strains, whereas the genome sequence clustered the isolate with NADC30-like viruses and it contains a discontinuous 131-aa deletion in NSP2 when compared to NADC30 strain. Notably, recombination analyses indicated that SCcd2020 is a multiple recombinant virus from NADC30-like, NADC34-like and JXA1-like strains, which is the first description of Chinese domestic HP-PRRSV involving the recombination event of an NADC34-like strain. Evolutionary analyses conducted on ORF5 sequences using Bayesian phylodynamic models estimated the time of emergence of NADC34-like strains in China was as early as July 2013, not long before its first detection in the United States in 2014. Importantly, an animal challenge study in 4-week-old piglets showed that SCcd2020 causes high fever and severe hemorrhagic pneumonia with pulmonary consolidation and edema, and it has a high mortality rate (60%), which indicated that SCcd2020 is a highly pathogenic PRRSV strain.Conclusions: This is the first report of the emergence of a novel highly pathogenic NADC34-like recombinant strain, and it highlights the importance of monitoring newly emerging PRRSV strains in China.
Decreased DIO3OS expression predicts poor prognosis in hepatocellular carcinoma and is associated with immune infiltration
Authors: Wang Yunhan , Sun Ping , Hao Xinping , Cao Daihong , Zhang Daijuan , Liu Jiangyue 
Published: July 2022

Abstract
Hepatocellular carcinoma has become one of the most shared cancers in the whole world because of its high morbidity, poor survival rate and low recovery rate. Especially in China, hepatocellular carcinoma has been regarded as one of the most malignant cancers. With the exploration of tumor diseases and the development of bioinformatics over the years, lncRNAs have been found to play a direct or indirect regulatory role in tumorigenesis and tumor development, which brings a new direction for the treatment of tumors. Previous studies had investigated that DIO3 opposite strand upstream RNA(DIO3OS) played a promoting role in the occurrence and development of thyroid carcinoma and uterine corpus endometrial carcinoma. However, research focusing on the role of DIO3OS in hepatocellular carcinoma is still uncleared. We found that low DIO3OS level often predicts poor prognosis in patients with hepatocellular carcinoma. In addition, there was significant correlation between different immune cells and their immune markers and DIO3OS. Our results suggest that DIO3OS can be used as an effective biomarker in the diagnosis and prognosis of hepatocellular carcinoma, affecting the development of hepatocellular carcinoma by influencing tumor immune cell infiltration.
Intraoperative management of mechanical ventilation in non-cardiothoracic surgery: a national survey in China
Authors: Rao Qian-Qian , Yu Hong , Wang Si-Yang , He Jia-Xin , Li Xue-Fei , Fei Fei , Shang Kai-Xi , Yu Hai 
Published: July 2022

Abstract
Background
The current clinical practice regarding intraoperative ventilation management in non-cardiothoracic surgical setting among anesthesia providers in China is unclear.
Methods
We developed a 25-question anonymous survey which was distributed to anesthesia departments from 81 tertiary/university hospitals in China between August 2021 and September 2021. Information including participant characteristics, ventilatory settings, and opinions on lung-protective ventilation were obtained by electronic survey. Results were reported descriptively.
Results
The survey was completed by 1089 anesthesiologists from 57 institutions (70% institution response rate). The vast majority of respondents (1010/1089; 92.7%)used low tidal volume (6–8 ml kg − 1) ventilation. About three quarters (840/1089; 77.1%) utilized positive end-expiratory pressure, and most anesthesiologists set positive end-expiratory pressure of 1–5 cmH2O in either laparoscopic (639/840; 76.1%) or non-laparoscopic surgery (736/840; 87.6%). The majority of respondents (921/1089; 84.6%) performed alveolar recruitment maneuver intraoperatively. Approximate 20% of respondents routinely implemented lung-protective ventilation with bundles of low tidal volume, positive end-expiratory pressure, and alveolar recruitment maneuver.
Conclusions
We found that heterogeneity exists between individual practices regarding intraoperative ventilation management in the setting of non-cardiothoracic surgery, indicating the potential knowledge-practice gap among Chinese anesthesiologists.
Trial registration number:
ChiCTR2100047653; Registration date: June 21, 2021 Registry URL: Chictr.org.cn.
Single-cell transcriptomics-based multidisease analysis revealing the molecular dynamics of retinal neurovascular units under inflammatory and hypoxic conditions
Authors: Zhang Yuxi , Yang Xiongyi , Deng Xiaoqing , Yang Siyu , Li Qiumo , Xie Zhuohang , Hong Libing , Cao Mingzhe , Yi Guoguo , Fu Min 
Published: July 2022

Abstract
The retinal neurovascular unit (NVU) is paramount to maintaining the homeostasis of the retina and determines the progression of various diseases, including diabetic retinopathy (DR), glaucoma, and retinopathy of prematurity (ROP). Although some studies have investigated these diseases, a combined analysis of disease-wide etiology in the NUV at the single-cell level is lacking. Herein, we constructed an atlas of the NVU under inflammatory and hypoxic conditions by integrating single-cell transcriptome data from retinas from wild-type, AireKO, and NdpKO mice. Based on the heterogeneity of the NVU structure and transcriptome diversity under normal and pathological conditions, we discovered two subpopulations of Müller cells: Aqp4hi and Aqp4lo cells. Specifically, Aqp4lo cells expresses phototransduction genes and represent a special type of Müller cell distinct from Aqp4hi cells, classical Müller cells. AireKO mice exhibit experimental autoimmune uveitis (EAU) with severe damage to the NVU structure, mainly degeneration of Aqp4hi cells. NdpKO mice exhibited familial exudative vitreoretinopathy (FEVR), with damage to the endothelial barrier, endothelial cell tight junction destruction and basement membrane thickening, accompanied by the reactive secretion of proangiogenic factors by Aqp4hi cells. In both EAU and FEVR, Aqp4hi cells are a key factor leading to NVU damage, and the mechanism by which they are generated is regulated by different transcription factors. By studying the pattern of immune cell infiltration in AireKO mice, we constructed a regulatory loop of "inflammatory cells/NVU - monocytes - APCs - Ifng+ T cells", providing a new target for blocking the inflammatory cascade. Our elucidation of the cell-specific molecular changes, cell‒cell interactions and transcriptional mechanisms of the retinal NVU provides new insights to support the development of multipurpose drugs to block or even reverse NVU damage.
The SDNoC Paradigm with Parallel Cores and Shortest Paths
Authors: Avelino Adelino , Oliveira Samuel , Kreutz Márcio 
Published: July 2022

Abstract
Networks-on-chip provides benefits for improving distributed applications performance due to their intrinsic parallelism in communications. One promising direction for optimizing such architectures is the software defined paradigm, the software defined Networks on Chip (SDNoCs). In this approach, all routing controller mechanisms run as software in a central controller. This work proposes the optimization of SDNoCs by either exploring different routing strategies and varying the number of controllers. The approach suggests a significant improvement in communication latency by reducing the waiting time of packets in the controllers queue. The results obtained are promising. Applying Dijkstra and Branch-and-Bound algorithms and increasing the number of controller cores optimize communication latency in 100\% of the cases when compared to the XY routing strategy.
C-type natriuretic peptide (CNP) potentiates the inhibitory effect of cisplatin on metastatic B16F10 melanoma cell proliferation and invasion by modulating inflammation and MMP-2/-9.
Authors: Sivakalai Suresh Dhanusu , Babu Aadhil Ashwaq , Kalaiarasu Lakshmi Priya , Vellaichamy Elangovan 
Published: July 2022

Abstract
In the present study, we evaluated whether C-type natriuretic peptide (CNP) alone or combined with cisplatin (CDDP) can inhibit cell proliferation and invasion of B16F10 cells. The cell viability assay, Western blot, qPCR, and Boyden chamber assays were performed to assess cell proliferation, inflammation, and invasion of B16F10 cells. Dose-dependent inhibition of cell proliferation was noticed in CNP alone treated cells, with 50% inhibition seen at 10μM of CNP. A significant reduction in cell proliferation markers (Cyclin D1 and PCNA, p<0.05), inflammation markers (NF-κB, TNF-α, and COX-2, p<0.05), and invasion markers (MMP-2/-9, p<0.05) were observed in B16F10 cells treated with CNP. A more prominent cell proliferation inhibition was noticed in a combination of CNP+CDDP treatment. Furthermore, when there was an increase in the concentration of CNP (5μM to 10μM) along with CDDP (2μM), strongly decreases the cell proliferation, inflammatory markers (p<0.01) and invasion (35% and 17%, p<0.01) of B16F10 cells as compared with CNP/CDDP alone treatment cells, respectively. The present study results demonstrate that CNP alone treatment potentially suppresses the cell proliferation, inflammation, and invasion of the B16F10 cells. Furthermore, to improve the inhibitory effects on cell proliferation of anti-cancer agents, we found that the combination of CNP and a non-cytotoxic concentration of CDDP increases the anti-proliferation and anti-invasion activity. Our results suggest that CNP possesses a unique role in inhibitory effects on cell proliferation and metastatic levels of B16F10 cells. Further, the CNP and CDDP combination may be helpful in the therapeutic purpose of cancer.
Removal of toluene from air- scream using semi industrial two-phase bioscrubber with cutting oil: An intervention study
Authors: Nourmohammadi Mohammad , Golbabaei Farideh Golbabaei , Karimi Ali , Taban Ebrahim 
Published: July 2022

Abstract
Toluene is a single-ring aromatic, which is widely used in various industrial processes. Regarding to harmful effects to human, the aim of this study was removal of toluene from the airflow using industrial-scale low-pressure two-phase bioscrubber with cutting oil. The design of the bioscrubber of the present study consisted of two parts of the adsorption column and a. Also, cutting oil was added at concentrations of 5%, 7.5% and 10% as organic phases and tested. The results show that after 72 hours with increasing organic phase concentration, toluene concentration was further decomposed compared to the control sample. The results showed that the use of 5% concentration of cutting oil increased the efficiency from 22% in the case of no organic phase to 55% in the case of using 5% of cutting oil. At a concentration of 7.5%, the lowest efficiency was 38% and the highest was 63%. Also, the minimum and maximum removal capacity are 17 and 269 g/m3.h, respectively. Efficiency at a concentration level of 5% of cutting oil as an organic phase is better than a concentration of 7.5%. In this study, for the first time in Iran, cutting oil was used as an organic phase, which showed that cutting oil in concentrations less than 10% had no effect on the growth of microorganisms. The results showed that shear oil and silicone oil increased the adsorption of volatile organic compounds in bioscrubber.
SiNi San Regulate TNBS-Induced Inflammatory Bowel Disease through Anti-Inflammation and Intestinal mucosal barrier protection
Authors: Zhang Yongpeng , An Yating , Li Yongmin , Li Yingchun , Zhao Huiqiao , He Wei 
Published: July 2022

Abstract
Severe inflammatory damage and permeability changes in colon are the main features of inflammatory bowel disease (IBD) which is a serious digestive system disease. SiNi San (SNS) originated from Treatise on Febrile Diseases, is usually used to treat gastrointestinal diseases. In this study, we explored the protective effect of SNS on IBD. IBD rats were induced by trinitrobenzene sulfonic acid. 125, 250 and 500 mg/kg of oral SNS significantly alleviated weight loss and diarrhea, reduced colitis inflammatory cell infiltration and inhibited pro-inflammatory cytokine production. SNS also revealed anti-inflammatory effects on lipopolysaccharide (LPS)-induced RAW cells. The mechanism may be related to the inhibition of tumor necrosis factor- α (TNF-α)-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway to suppress cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase(iNOS) expressions. Also colons were collected for immunofluorescence assay and LPS-induced cell-co-culture system of Caco-2 and RAW264.7 cells were used to determine protective effect on intestinal epithelial monolayers damage. Our results showed SNS enhanced expression of claudin-2 and zona occludens-1 (ZO-1), elevated trans epithelial electric resistance, decreased the permeability in LPS-damaged epithelial monolayers system. SNS showed regulatory effect on IBD by anti-inflammatory and protecting intestinal mucosal barrier. It is suggested SNS may be new supplements against IBD.
Corrosion Behavior and Wear Resistance for Ti-18Nb-xCu Alloys
Authors: Demirtaş Hüseyin , Riyadh Mohammed , Anaee Rana 
Published: July 2022

Abstract
Corrosion behavior and wear resistance were investigated for three prepared alloys (Ti-18Nb-xCu) by powder metallurgy method with adding three weight percents of Cu (5, 7 and 9 wt %). The wear results show that Ti-18Nb-7Cu alloy has the lowest wear rate due to hard Ti2Cu phase which mostly formed in the percent of 7%Cu. While the corrosion behavior was different due to the variation in formed cathodic and anodic sites and the formed phase led to increasing the formation of micro-galvanic cells on the alloys surface. AFM analysis was applied to show the surface topography and measure surface roughness, the results indicated that Ti alloy with 7% Cu had the highest surface roughness.
Successful Treatment of Inflammatory Myofibroblastic Tumor with Surgical Residue by Radiochemotherapy: Case Report and Review of the Literature
Authors: Guo Haiwei , Jiang Mingyun , Cai Juanjuan , Liu Ruiqi , Yao Weiping , Liang Xiaodong , Zhang Haibo 
Published: July 2022

Abstract
Background: Inflammatory myofibroblastic tumors (IMTs) are rare soft-tissue neoplasms. Accordingly, there is no standardized therapy for unresectable or advanced IMT. Chemotherapy, radiotherapy, and targeted molecular therapy play an important role in unresectable or advanced IMT.Case presentation: We present a 54-year-old man with a cough and chest distress case report. The thoracic surgeon performed the right upper pulmonary occupying lesion wedge resection and enlarged lymph node excision biopsy. Pathologic diagnosis revealed that the morphology of "right upper lung mass" was considered as Inflammatory Myofibroblastic Tumor (IMT). Radiotherapy was indicated at a high dose: 5400cGy in 27 fractions of 2Gy over 5 weeks were delivered combined with cisplatin. The patient was given a CT/MRI and hematological index every 3 months and experienced no more adverse events. The patient survives with no tumor recurrence as of the last follow-up. Progression-free survival (PFS) exceeded 48 months.Conclusions: We have reviewed the literature and summarized and discussed the radiotherapy treatment options and challenges for IMT. We first reported high-dose radiotherapy combined with chemotherapy treatment for unresectable IMT. Concurrent radiochemotherapy may be considered an intensive treatment for local progress, local recurrence, and nonresectable IMT patients.
Strong and tough Mg-MAX phase composites with nacre-like lamellar and brick-and-mortar architectures
Authors: Liu Yanyan , Xie Xi , Liu Zengqian , Yu Qin , Wang Xuegang , Jia Qing , Zhang Zhefeng , Yang Rui , Ritchie Robert 
Published: July 2022

Abstract
Bioinspired nacre-like structures are effective in toughening materials, yet are difficult to construct in Mg-ceramic systems. Here, a set of Mg-MAX phase composites with nacre-like lamellar and brick-and-mortar architectures were fabricated by pressureless infiltration of the Mg melt into ice-templated ceramic scaffolds. The structure and mechanical properties of the composites were elucidated with a special focus on the effects of the types of architectures (lamellar or brick-and-mortar) and matrices (pure Mg or Mg alloy) on the toughening mechanisms. The nacre-like architectures were found to play a role in blunting the cracks via plastic deformation and microcracking, and shielding the cracks from applied stress by promoting crack deflection and uncracked-ligament bridging mechanisms. These composites achieved a good combination of (specific) strength and fracture toughness which are superior to other reported Mg-ceramic and nacre-like metal-ceramic composite materials.
Love in Early Childhood Education: the experience of Aotearoa New Zealand
Authors: Stewart-MacKenzie Helen Lorraine Grace 
Published: July 2022

Abstract
Love is essential for human psycho-social development, in particular in the early years. Just as the love of parents/care-givers and others in the home setting is vital, there is increasing recognition that love is important for infants, toddlers and young children in early childhood education settings (ECES). However, love demonstrated by teaching professionals continues to be under-researched (Dalli, 2006; Page, 2017a). As a result, educational policy and practice guidelines are still embryonic, or may not yet exist (Dalli et al, 2011; Hughes, 2013). This article presents findings from a nation-wide survey of early childhood professionals in Aotearoa New Zealand (NZ) undertaken in 2020 which explored early childhood teachers’ understandings of love in ECES in NZ; the first such survey undertaken. Respondents emphasised that a professional approach to love is important despite many responses indicating love is still viewed as unprofessional by early childhood education (ECE) teachers and managers. A key finding of this research is that understanding the nature of love in ECES is required in order to legitimise love in professional spaces and thereby inform policy and practice.