bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factores asociados a sobrevida de fetos con diagnóstico prenatal de megavejiga
Authors: Sierralta Born Maria Consuelo , Moncada Vidal Karen , Rodríguez Herrera Jorge , Cisternas Olguín Daniela , Ossandón Correa Francisco , Rodríguez Aris Juan Guillermo 
Published: February 2022

El hallazgo de megavejiga en una ecografía prenatal sugiere una obstrucción mecánica o funcional al vaciamiento vesical, condición infrecuente y habitualmente de mal pronóstico. Objetivo: determinar factores asociados a sobrevida en pacientes con diagnóstico prenatal de megavejiga. Pacientes y Método: Estudio retrospectivo de fetos con diagnóstico prenatal de megavejiga, entre los años 2003 y 2018 en Centro de Referencia Perinatal Oriente (CERPO), Universidad de Chile. Se analizaron variables prenatales y postnatales, diagnóstico etiológico, hipoplasia pulmonar, tratamiento médico quirúrgico, mortalidad, función renal y terapia de reemplazo renal. La variable principal analizada fue la sobrevida al año, y las variables secundarias fueron la función renal y los factores predictores de sobrevida. Se realizó un análisis univariado con Test de U Mann Whitney y Fisher, y se definió como estadísticamente significativo un p < 0,05. Resultados: Se incluyeron 25 fetos con diagnóstico prenatal de megavejiga. El 52% presentó oligohidroamnios y un 84% alteraciones renales. Se realizó vesicocentesis diagnóstica en 15 fetos, con instalación de shunt derivativo vesicoamniótico en 5 casos. Hubo 6 mortinatos (24%), y de los 19 nacidos vivos 9 fallecieron en el período neonatal precoz (36%) y uno a los 8 meses de edad de causa no nefrourológica. Nueve pacientes sobrevivieron al año de vida (36%), 2 pacientes sanos sin alteración nefrourológica y 7 pacientes presentaron patología nefrourológica. Dos pacientes evolucionaron con enfermedad renal crónica terminal. Sólo la presencia de hipoplasia pulmonar se relacionó con mayor mortalidad perinatal (p < 0,05) asociada en todos los casos a oligohidroamnios. El oligohidroamnios no fue un factor de mal pronóstico en esta serie. Conclusiones: El diagnóstico prenatal de megavejiga comprende un amplio espectro de patologías, desde condiciones con alta mortalidad perinatal a pacientes sanos con dilatación vesical transitoria sin patología nefrourológica. Se identificó como único factor asociado a mayor mortalidad la presencia de hipoplasia pulmonar.
Síndrome de ACNES, explorando la pared abdominal como noxa del dolor abdominal
Authors: García R. Clara , Botija A. Gonzalo , Recio L. Aránzazu , Nieto I. Consuelo , Barrio M. Alfonso 
Published: February 2022

El dolor de pared abdominal, y concretamente el síndrome de ACNES (Anterior Cutaneous Nerve Entrapment Syndrome), forma parte del diagnóstico diferencial del dolor abdominal crónico. Este síndrome es poco conocido y por consiguiente infradiagnosticado. Objetivos: Describir las características clínicas y evolutivas de pacientes diagnosticados de ACNES y llamar la atención sobre esta patología. Pacientes y Método: Estudio descriptivo retrospectivo realizado en un centro de referencia, entre octubre 2016 y julio de 2021, en pacientes menores 17 años, diagnosticados de ACNES, que cumpliesen al menos dos de cuatro hallazgos siguientes: signo de Carnett, Pinch test, disestesias en el punto de máximo dolor, mejoría tras infiltración de anestésico local, habiéndose descartado patología abdominal visceral o funcional. Se recogieron variables epidemiológicas, síntomas, exploración física, pruebas complementarias, tratamiento y evolución. Se usaron estadísticas descriptivas. Resultados: 20 pacientes diagnosticados de ACNES, 75% mujeres, mediana de edad 12,85 años. Se objetivó signo de Carnett en 95%, signo de Pinch test en 65% y disestesias en 90% de los pacientes. 65% refirió síntomas pseudoviscerales. 7 pacientes presentaron sobrepeso u obesidad. La localización más frecuente (50%) fue la fosa ilíaca derecha, a nivel T10-T11. Un paciente refirió mejoría espontánea; 7 mejoraron con analgesia oral; 9 fueron derivados a la Unidad del Dolor, de los cuales acudieron 5, los que mejoraron con infiltración anestésica con bupivacaína-triamcinolona; los 4 restantes perdieron seguimiento. Conclusión: El ACNES debe ser considerado en pacientes con dolor abdominal crónico. La combinación de hallazgos típicos en la historia y el examen físico permiten su diagnóstico, evitando pruebas complementarias innecesarias. El tratamiento debe ser escalonado, comenzando por analgesia oral, seguido de infiltración anestésica y, por último, neurectomía anterior.
Hemiplejia Alternante de la infancia asociada a variante patogénica del gen ATP1A3
Authors: Sandoval Francisca , López Francisca 
Published: February 2022

La hemiplejia alternante de la infancia (HAI) es una enfermedad rara caracterizada por episodios de hemi/cuadriplejia, posturas distónicas, movimientos oculares anormales y trastornos del movimiento. Las mutaciones del gen ATP1A3 son las más frecuentemente asociadas a HAI. Objetivo: Exponer un caso clínico de HAI, en el que el estudio genético y la observación de videos caseros fueron de gran utilidad diagnóstica. Caso Clínico: Paciente de sexo femenino, quien a los 3 meses de vida presentó varios episodios de posturas distónicas, clonías de extremidades, versión cefálica y desviación lateral de la mirada, de varios minutos de duración. Inicialmente se diagnosticó epilepsia y se inició tratamiento con levetiracetam, sin mejoría. Se realizó EEG y RM cerebral que resultaron normales. Con esto, se descartó epilepsia y se sospechó distonía transitoria del lactante. Sin embargo, los eventos se hicieron más frecuentes, de mayor duración y característicamente cedían durante el sueño. Los familiares aportaron videos caseros aclaratorios de los eventos. A los 6 meses de vida cursó con hemiparesia transitoria alternante. El panel genético de distonías evidenció variante patogénica del gen ATP1A3, confirmando diagnóstico de HAI. Se inició tratamiento con flunarizina con buena respuesta clínica a los 12 meses de seguimiento. Conclusiones: El diagnóstico de HAI es complejo y se confunde frecuentemente con epilepsia. Es importante realizar correctamente el diagnóstico diferencial, incluyendo anamnesis, exámenes como EEG y la minuciosa observación de los eventos clínicos, que con el actual acceso a tecnología audiovisual se hace más asertivo. El análisis genético constituye una gran herramienta diagnóstica que realizado a tiempo evita otros estudios y terapias innecesarias.
DISEÑO GRÁFICO Y PATRIMONIO
Authors: Lazo-Galán Juan 
Published: February 2022

El diseño gráfico profesional en Ecuador se ha convertido en una carrera que tiene una enorme aceptación en el país con más de 24 universidades que tienen la carrera. Es una profesión que utiliza signos y símbolos arraigados en el inconsciente colectivo para generar comunicación. En un importante movimiento en los últimos años, los diseñadores gráficos han estado buscando referentes dentro del patrimonio ecuatoriano para generar propuestas con características propias, en algunos casos volviendo la mirada al pasado y en otras buscando en la cultura popular y en la naturaleza aquello que nos caracteriza. Este artículo analiza el diseño gráfico generado en los últimos 50 años y su relación con el patrimonio histórico, cultural y natural del país.
Palabras Clave: Cultura popular, precolombino, naturaleza, identidad, expresión visual
AbstractProfessional graphic design has become a career with a huge acceptance in Ecuador, with more than 24 universities offering it as a program of study. It’s a profession that uses signs and symbols rooted in the collective subconscious to producecommunication. In an important movement over the last few years, graphic designers have been searching for references within Ecuadorean culture to generate proposals with their own unique characteristics, in some cases, looking at the past andin others, searching within popular culture and in nature all that characterizes us. This article analyzes graphic design generated in the last 50 years and its relationship with the country’s historical, cultural and natural heritage.
Keywords: Popular culture, pre-Columbian, nature, identity, visual expression.
Pelatihan Pembuatan Vermikompos di Desa Lokasi Baru Kecamatan Air Periukan Kabupaten Seluma
Authors: Trisusilo Agung , Cahyadinata Indra 
Published: February 2022

This activity aims to increase the awareness of the people of Lokasi Baru Village to make animal manure into compost using the vermicomposting method. The method used in this activity is a combination of counseling and structured training. The result of this activity is that participants are able to see the potential resources they have in the form of animal manure and process it into vermicompost fertilizer which is more useful and has economic value. In practice, participants experienced problems at the stage of preparation of developmental media, hatching of worm eggs, and the composting process. Although in the implementation process the participants faced several obstacles, with assistance from the service team they were able to resolve these obstacles independently. After participating in this series of activities, the participants learned that the livestock waste they produce has economic value if managed properly. Therefore, at this time they have been practicing the knowledge gained independently. Keywords: shit livestock; Lumbricus sp.; vermicompost
Glucose-Derived Raspberry Ketone Produced via Engineered Escherichia coli Metabolism
Authors: Masuo Shunsuke , Saga Chisa , Usui Kurumi , Sasakura Yuma , Kawasaki Yukie , Takaya Naoki 
Published: February 2022

The demand for raspberry ketone (RK) as a plant-based natural flavoring agent is high, but natural RK is one of the most expensive flavor compounds due to its limited content in plants. Here, we produced RK de novo from simple carbon sources in Escherichia coli. We genetically engineered E. coli metabolism to overproduce the metabolic precursors tyrosine and p-coumaric acid and increase RK production. The engineered E. coli produced 19.3- and 1.9 g/L of tyrosine and p-coumaric acid from glucose, respectively. The p-coumaric acid CoA ligase from Agrobacterium tumefaciens and amino acid substituted benzalacetone synthase of Rhemu palmatum (Chinese rhubarb) were overexpressed in E. coli overproducing p-coumaric acid. The overexpression of fabF, encoding β-ketoacyl-acyl carrier protein synthetase II increased intracellular malonyl-CoA, the precursor of benzalacetone synthase for RK biosynthesis, and improved RK production. Fed-batch cultures given glucose as a carbon source produced 62 mg/L of RK under optimized conditions. Our production system is inexpensive and does not rely on plant extraction; thus, it should significantly contribute to the flavor and fragrance industries.
Cysteine Substitution and Calcium-Binding Mutations in FBN1 cbEGF-Like Domains Are Associated With Severe Ocular Involvement in Patients With Congenital Ectopia Lentis
Authors: Zhang Min , Chen Zexu , Chen Tianhui , Sun Xiaodong , Jiang Yongxiang 
Published: February 2022

Purpose: To investigate the clinical manifestations of congenital ectopia lentis (CEL) in patients with fibrillin (FBN1) calcium-binding epidermal growth factor (cbEGF)-like mutations.Design: Retrospective cohort study.Methods: Consecutive 68 CEL probands with FBN1 cbEGF-like mutations were recruited, mostly comprising Marfan syndrome (MFS) patients. Patients were classified into the cysteine group (n = 43), calcium (Ca2+)-binding group (n = 13) or the others (n = 12) according to their genotypes. Ocular biometrics, morbidities and visual performance were compared among different mutation groups. Linear regression was used to evaluate the risk factors for axial length (AL) elongation.Results: With age-adjustment, cysteine substitution and Ca2+-binding mutations positively contributed to AL elongation (standardized coefficient: 0.410 and 0.367, p = 0.008 and 0.017, respectively). In addition, cataract formation was more frequently detected in patients with Ca2+-binding mutations (observed n = 3, expected n = 1.0; p = 0.036). Patients with cysteine substitutions had the poorest preoperative visual acuity among the three groups (p = 0.012) and did not recover as well as other patients. More MFS diagnoses were made in patients with cysteine substitutions (observed n = 16, expected n = 12.6), while ectopia lentis syndrome was detected more often in patients with cbEGF-like mutations out of the functional regions (observed n = 6, expected n = 2.5; p = 0.023).Conclusion: Compared with patients with cbEGF-like mutations out of functional regions, patients with cysteine substitutions or Ca2+-binding mutations had longer ALs with age adjustment, poorer ocular involvement, visual performance, and systematic manifestations.
Identification of DDX31 as a Potential Oncogene of Invasive Metastasis and Proliferation in PDAC
Authors: Xie Yongjie , Liu Yang , Ding Jinsheng , Li Guangming , Ni Bo , Pang Huifang , Hu Xin , Wu Liangliang 
Published: February 2022

Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest malignant tumors worldwide and has poor prognosis. DEAD box proteins31 (DDX31) participate in cellular processes involving RNA secondary structure changes. However, the functions of DDX31 in PDAC remain to be elucidated.Methods: The key gene DDX31 was identified using a combination of a risk model and weighted gene co-expression network analysis (WGCNA) with R software. The biological functions of DDX31 in PDAC were investigated through bioinformatics analysis and in vitro experiments.Results: Combining with WGCNA and risk model, DDX31 was identified as a potential factor of the invasive metastasis properties of PDAC, and its expression was closely related to the malignant differentiation of PDAC. The results of gene set enrichment analysis (GSEA) showed that DDX31 was correlated with cell invasive metastasis and proliferation by activating MAPK signaling pathway. The inhibition of DDX31 inhibited the invasion and migration of PDAC cells. Survival analysis showed that DDX31 expression was negatively associated with the poor prognosis in patients with PDAC.Interpretation:DDX31 may be a potential factor for PDAC. The inhibition of DDX31 may be a potential way to treat PDAC.
Effect of 2D and 3D Culture Microenvironments on Mesenchymal Stem Cell-Derived Extracellular Vesicles Potencies
Authors: Kusuma Gina D. , Li Anqi , Zhu Dandan , McDonald Hannah , Inocencio Ishmael M. , Chambers Daniel C. , Sinclair Kenneth , Fang Haoyun , Greening David W. , Frith Jessica E. , Lim Rebecca 
Published: February 2022

Therapeutic benefits of mesenchymal stem cells (MSCs) are now widely believed to come from their paracrine signalling, i.e. secreted factors such as cytokines, chemokines, and extracellular vesicles (EVs). Cell-free therapy using EVs is an active and emerging field in regenerative medicine. Typical 2D cultures on tissue culture plastic is far removed from the physiological environment of MSCs. The application of 3D cell culture allows MSCs to adapt to their cellular environment which, in turn, influences their paracrine signalling activity. In this study we evaluated the impact of 3D MSCs culture on EVs secretion, cargo proteome composition, and functional assessment in immunomodulatory, anti-inflammatory and anti-fibrotic properties.MSC-EVs from 2D and 3D cultures expressed classical EV markers CD81, CD63, and CD9 with particle diameter of &lt;100 nm. There were distinct changes in immunomodulatory potencies where 3D cultures exhibited reduced indoleamine 2,3-dioxygenase (IDO) activity and significantly reduced macrophage phagocytosis. Administration of 2D and 3D EVs following double dose bleomycin challenge in aged mice showed a marked increase of bodyweight loss in 3D group throughout days 7–28. Histopathological observations of lung tissues in 3D group showed increased collagen deposition, myofibroblast differentiation and leukocytes infiltrations. Assessment of lung mechanics showed 3D group did not improve lung function and instead exhibited increased resistance and tissue damping. Proteome profiling of MSC-EV composition revealed molecular enrichment of EV markers (compared to parental cells) and differential proteome between EVs from 2D and 3D culture condition associated with immune-based and fibrosis/extracellular matrix/membrane organization associated function.This study provides insight into distinct variation in EV protein composition dependent on the cellular microenvironment of the parental cells, which could have implications in their therapeutic effect and potency. Overall, this work suggests that EVs produced from 3D MSC cultures did not enhance typical MSC-EV properties expected from 2D cultures (immunomodulation, anti-fibrotic, anti-inflammatory). The outcome highlights critical differences between MSC-EVs obtained from different culture microenvironments, which should be considered when scaling up MSC culture for clinical manufacturing.
Huoshanmycins A‒C, New Polyketide Dimers Produced by Endophytic Streptomyces sp. HS-3-L-1 From Dendrobium huoshanense
Authors: Zhu Youjuan , Kong Yichao , Hong Yu , Zhang Ling , Li Simin , Hou Shurong , Chen Xiabin , Xie Tian , Hu Yang , Wang Xiachang 
Published: February 2022

Three new polyketide dimers named huoshanmycins A‒C (1–3) were isolated from a plant endophytic Streptomyces sp. HS-3-L-1 in the leaf of Dendrobium huoshanense, which was collected from the Cultivation base in Jiuxianzun Huoshanshihu Co., Ltd. The dimeric structures of huoshanmycins were composed of unusual polyketides SEK43, SEK15, or UWM4, with a unique methylene linkage. Their structures were elucidated through comprehensive 1D-/2D-NMR and HRESIMS spectroscopic data analysis. The cytotoxicity against MV4-11 human leukemia cell by the Cell Counting Kit-8 (CCK8) method was evaluated using isolated compounds with triptolide as positive control (IC50: 1.1 ± 0.4 μM). Huoshanmycins A and B (1, 2) displayed moderate cytotoxicity with IC50 values of 32.9 ± 7.2 and 33.2 ± 6.1 μM, respectively.
Prognostication of Pancreatic Cancer Using The Cancer Genome Atlas Based Ferroptosis-Related Long Non-Coding RNAs
Authors: Li Jiayu , Zhang Jinghui , Tao Shuiliang , Hong Jiaze , Zhang Yuyan , Chen Weiyan 
Published: February 2022

Background: Long non-coding RNAs (lncRNAs) are key regulators of pancreatic cancer development and are involved in ferroptosis regulation. LncRNA transcript levels serve as a prognostic factor for pancreatic cancer. Therefore, identifying ferroptosis-related lncRNAs (FRLs) with prognostic value in pancreatic cancer is critical.Methods: In this study, FRLs were identified by combining The Cancer Genome Atlas (TCGA) and FerrDb databases. For training cohort, univariate Cox, Lasso, and multivariate Cox regression analyses were applied to identify prognosis FRLs and then construct a prognostic FRLs signature. Testing cohort and entire cohort were applied to validate the prognostic signature. Moreover, the nomogram was performed to predict prognosis at different clinicopathological stages and risk scores. A co-expression network with 76 lncRNA-mRNA targets was constructed.Results: Univariate Cox analysis was performed to analyze the prognostic value of 193 lncRNAs. Furthermore, the least absolute shrinkage and selection operator and the multivariate Cox analysis were used to assess the prognostic value of these ferroptosis-related lncRNAs. A prognostic risk model, of six lncRNAs, including LINC01705, AC068620.2, TRAF3IP2-AS1, AC092171.2, AC099850.3, and MIR193BHG was constructed. The Kaplan Meier (KM) and time-related receiver operating characteristic (ROC) curve analysis were performed to calculate overall survival and compare high- and low-risk groups. There was also a significant difference in survival time between the high-risk and low-risk groups for the testing cohort and the entire cohort, with AUCs of .723, .753, respectively. Combined with clinicopathological characteristics, the risk model was validated as a new independent prognostic factor for pancreatic adenocarcinoma through univariate and multivariate Cox regression. Moreover, a nomogram showed good prediction.Conclusion: The signature of six FRLs had significant prognostic value for pancreatic adenocarcinoma. They may be a promising therapeutic target in clinical practice.
Pregnancy in Women With Preexisting Glomerular Diseases: A Single-Center Experience
Authors: Marinaki Smaragdi , Tsiakas Stathis , Skalioti Chrysanthi , Kapsia Eleni , Lionaki Sophia , Vallianou Kalliopi , Boletis John 
Published: February 2022

AimWomen with glomerular diseases are often of childbearing age. Besides lupus nephritis, data regarding pregnancy in patients with glomerular diseases are limited, posing a challenging task to attending nephrologists. This study aimed to investigate the pregnancy outcomes and the impact on the underlying glomerular disease among women followed in our institution.MethodsA single-center retrospective cohort study of women with biopsy-proven glomerular diseases who experienced pregnancy between 2010 and 2020. We analyzed data before, during, and after gestation.ResultsA total of 22 women, 13 women with primary and 9 women with secondary glomerular diseases, were included in this study. Most patients (82%) had received immunosuppressive treatment at various times before pregnancy. All the women were in remission, either complete (62%) or partial (38%), with well-preserved renal function (82%) before conception. A total of 30 live births and 1 stillbirth were recorded; the rate of preterm delivery was 23%. Renal function and proteinuria remained stable during pregnancy. Preeclampsia was observed in 6.7% of patients and disease relapse in 6.9% of the pregnancies.ConclusionPregnancy was associated with a low frequency of adverse events in women with underlying glomerular diseases, provided they have quiescent disease and preserved renal function.
Human T-Cell Lymphotropic Virus Type 1 and Strongyloides stercoralis Co-infection: A Systematic Review and Meta-Analysis
Authors: Ye Lingqing , Taylor Graham P. , Rosadas Carolina 
Published: February 2022

BackgroundThe distribution of human T cell lymphotropic virus type 1 (HTLV-1) overlaps with that of Strongyloides stercoralis. Strongyloides stercoralis infection has been reported to be impacted by co-infection with HTLV-1. Disseminated strongyloidiasis and hyperinfection syndrome, which are commonly fatal, are observed in HTLV-1 co-infected patients. Reduced efficacy of anti-strongyloidiasis treatment in HTLV-1 carriers has been reported. The aim of this meta-analysis and systematic review is to better understand the association between HTLV-1 and S. stercoralis infection.MethodsPubMed, Embase, MEDLINE, Global Health, Healthcare Management Information Consortium databases were searched. Studies regarding the prevalence of S. stercoralis, those evaluating the frequency of mild or severe strongyloidiasis, and treatment response in people living with and without HTLV-1 infection were included. Data were extracted and odds ratios were calculated. Random-effect meta-analysis was used to assess the pooled OR and 95% confidence intervals.ResultsFourteen studies were included after full-text reviewing of which seven described the prevalence of S. stercoralis and HTLV-1. The odds of S. stercoralis infection were higher in HTLV-1 carriers when compared with HTLV-1 seronegative subjects (OR 3.2 95%CI 1.7–6.2). A strong association was found between severe strongyloidiasis and HTLV-1 infection (OR 59.9, 95%CI 18.1–198). Co-infection with HTLV-1 was associated with a higher rate of strongyloidiasis treatment failure (OR 5.05, 95%CI 2.5–10.1).ConclusionStrongyloides stercoralis infection is more prevalent in people living with HTLV-1. Co-infected patients are more likely to develop severe presentation and to fail treatment. Screening for HTLV-1 and Strongyloides sp. should be routine when either is diagnosed.