bluuebunny/crossref_metadata_2025_split huggingface dataset:
KEPERCAYAAN MASYARAKAT JAWA DALAM NOVEL KKN DI DESA PENARI KARYA SIMPLEMAN: SEBUAH KAJIAN SIMBOLIK CLIFFORD GEERTZ
Authors: Fanisia Like , Fathurrozi Moh Alex , Yudistian Putri Aulia , Damariswara Rian 
Published: September 2022

Penelitian ini bertujuan untuk mendeskripsikan kepercayaan masyarakat jawa bagian timur yang dikhususkan pada kepercayaan terhadap makhluk halus dan makna simbolik dari budaya jawa yang telah dilakukan. Data dalam penelitian ini berwujud frase, kalimat, dan ungkapan yang terdapat dalam novel KKN di Desa Penari karya Simpleman. Sedangkan, sumber data yang digunakan dalam penelitian ini yaitu novel KKN di Desa Penari karya Simpleman. Teknik analisis data menggunakan teknik deskriptif analisis. Teknik deskriptif analisis dilakukan dengan mendeskripsikan data-data yang ada kemudian disusul dengan analisis menggunakan Interpretatif simbolik Clifford Geertz. Hasil penelitian ini yaitu sebagai berikut. (1) Kepercayaan terhadap makhluk halus jenis gendruwo, (2) makna simbolik budaya Jawa yang terepresentasi dalam novel KKN di desa penari karya Simplemen ditunjukan 7 macam hal yaitu, (a) tapak tilas, (b) pemakaman, (c) sendang, (d) sesajen, (e) tempat wingit, (f) tumbal, (g) gamelan.
Evidence-Based Veterinary Medicine Learning: an open access tutorial for practitioners and students
Authors: Brown Fiona J L , Moberly Heather K , Place Emma 
Published: September 2022

EBVM Learning, an open access online tutorial, has been developed to support the teaching of evidence-based veterinary medicine (EBVM). This paper provides the project background and teaching examples. The authors request JEAHIL readers share this resource with colleagues who support evidence-based practices including veterinary medicine.
A GIANT BLADDER STONE IN A YOUNG FISHERMAN FROM MOLUCCAS: A CASE REPORT
Authors: Salim Rakha Sulthan , Tuasikal Rabinda Fitriana , Tualeka Mo 
Published: September 2022

Objective: To present a rare case of a giant bladder stone. Case(s) Presentation: We report a 20-year-old fisherman, with excessive exposure to sunlight, poor fluid intake, and unhealthy lifestyle, who came to the hospital clinic with a 2-year-history of progressing dysuria, frequency, and hematuria. Investigations revealed a giant bladder stone without bladder outlet obstruction. Open cystolithotomy was performed and a giant stone with 13.2x8x8.3 cm in size was extracted without adhesion to the bladder wall. Discussion: A Giant bladder stone is a rare disease and fewer than 100 cases have been reported globally. Patients commonly are accompanied by multifactorial conditions and have a wide range of presentations. Conclusion: This case presents a giant bladder stone without bladder outlet obstruction in a young adult, it is considered that the stone rose due to other significant factors that followed the patient.
THE ROLE OF R.E.N.A.L NEPHROMETRY SCORE TO PREDICT PERIOPERATIVE OUTCOME FOLLOWING PARTIAL NEPHRECTOMY IN A NATIONAL REFERRAL HOSPITAL
Authors: Soeroto Adhitama Alam , Mochtar Chaidir A , Umbas Rainy , Hamid Agus Rizal AH 
Published: September 2022

Objective: This study aim to see the significance of R.E.N.A.L-NS in our center. Material & Methods: We retrospectively collected all the data of RCC patients that underwent partial nephrectomy (PN) in Cipto Mangunkusumo Hospital from January 2010-January 2018, with complete CT-scan examination. Patients with single kidney were excluded from our study. We evaluated intraoperative blood loss, length of operation, post operative length of stay, post operative kidney function, and complications as the perioperative parameters. Patients were categorized into 3 category based their complexity from RENAL-NS into: low (4-6), moderate (7-9), and high (10-12). Complications were based on Clavien-Dindo classification. Perioperative outcomes were analyzed based on the stratification of the patients and analyzed using ANOVA and chi-square. Results: We evaluated 25 partial nephrectomy cases which are suitable with inclusion and exclusion criteria. There were 8 (32%) low, 14 (56%) medium, and 3 (12%) high complexity cases. Median age of patients were 61 (46-71) years old, with mean tumor diameter 72.07 (+ 38.9) mm. Nine (36%) patients underwent open procedure and 16 (64%) underwent laparoscopic procedure. The laparoscopic procedure was tend to the lower complexity of R.E.N.A.L score (p=0.048). Higher complexity of R.E.N.A.L score correlated with higher blood loss (p<0.001), length of stay (p<0.001), complication rate (p<0.001), and length of operation (p=0.033). Conclusion: R.E.N.A.L-NS has a role for a selection of type of procedure and a prediction of perioperative outcome in partial nephrectomy. R.E.N.A.L-NS can be used in daily basis as it could determine the procedure and several outcomes of the partial nephrectomy procedure.
Simultaneous pre-treatment of dietary Moringa oleifera leaf meal with polyethylene glycol and fibrolytic enzymes: Effect on growth performance, physiological indices, and meat quality parameters in jumbo quail
Authors: Mulaudzi Anzai , Mnisi Caven Mguvane , Mlambo Victor 
Published: September 2022

The utility of Moringa oleifera leaf meal (MOLM) as a source of biologically active substances and nutrients for Jumbo quail is limited by high concentrations of condensed tannins and fiber. Simultaneous application of polyethylene glycol (PEG), a tannin-binding compound, and exogenous fibrolytic multi-enzymes could ameliorate antinutritional effects of condensed tannins and fiber thus improving MOLM utilization in quail diets. This study investigated the effect of pre-treating dietary MOLM with PEG and fibrolytic enzymes on live performance, blood parameters, visceral organs, and carcass and meat quality characteristics in Jumbo quail. A total of 381, two-week-old quail chicks (57.5 ± 3.95 g live-weight) were randomly distributed to six dietary treatments replicated six times. The treatments were: T1 = a standard grower diet containing untreated MOLM (10%); T2 = a standard grower diet containing MOLM (10%) pre-treated with 5.4% PEG; and a standard grower diet containing MOLM (10%) pre-treated with 5.4% PEG and 1.25% (T3), 1.50% (T4), 1.75% (T5) and 2.0% (T6) fibrolytic multi-enzymes. Graded levels of enzymes did not induce linear or quadratic effects for overall feed intake, feed conversion efficiency, hematological, carcass, and meat quality parameters in response to increasing fibrolytic enzyme levels. However, weight gain in week 3 quadratically responded [R2 = 0.117, P = 0.043]. Three-week old birds reared on T3 had lower (p &lt; 0.05) weight gains (40.9 g/bird) than those reared on the other treatment groups. Birds reared on T6 diet had longer caecum (14.1 cm) than those reared on T2, T3, T4 and T5 whose caeca lengths did not differ (P &gt; 0.05). Birds reared on diet T5 had shorter small intestines (59.5 cm) than those reared on T1 and T2 diets. It can be concluded that simultaneous pre-treatment of dietary MOLM with PEG and fibrolytic enzymes did not improve live performance, blood parameters, and carcass and meat quality traits, but affected some visceral organ sizes in Jumbo quail.
Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
Authors: González-Gamboa Ivonne , Velázquez-Lam Edith , Lobo-Zegers Matías José , Frías-Sánchez Ada Itzel , Tavares-Negrete Jorge Alfonso , Monroy-Borrego Andrea , Menchaca-Arrendondo Jorge Luis , Williams Laura , Lunello Pablo , Ponz Fernando , Alvarez Mario Moisés , Trujillo-de Santiago Grissel 
Published: September 2022

Current tissue engineering techniques frequently rely on hydrogels to support cell growth, as these materials strongly mimic the extracellular matrix. However, hydrogels often need ad hoc customization to generate specific tissue constructs. One popular strategy for hydrogel functionalization is to add nanoparticles to them. Here, we present a plant viral nanoparticle the turnip mosaic virus (TuMV), as a promising additive for gelatin methacryloyl (GelMA) hydrogels for the engineering of mammalian tissues. TuMV is a flexuous, elongated, tubular protein nanoparticle (700–750 nm long and 12–15 nm wide) and is incapable of infecting mammalian cells. These flexuous nanoparticles spontaneously form entangled nanomeshes in aqueous environments, and we hypothesized that this nanomesh structure could serve as a nanoscaffold for cells. Human fibroblasts loaded into GelMA-TuMV hydrogels exhibited similar metabolic activity to that of cells loaded in pristine GelMA hydrogels. However, cells cultured in GelMA-TuMV formed clusters and assumed an elongated morphology in contrast to the homogeneous and confluent cultures seen on GelMA surfaces, suggesting that the nanoscaffold material per se did not favor cell adhesion. We also covalently conjugated TuMV particles with epidermal growth factor (EGF) using a straightforward reaction scheme based on a Staudinger reaction. BJ cells cultured on the functionalized scaffolds increased their confluency by approximately 30% compared to growth with unconjugated EGF. We also provide examples of the use of GelMA-TuMV hydrogels in different biofabrication scenarios, include casting, flow-based-manufacture of filaments, and bioprinting. We envision TuMV as a versatile nanobiomaterial that can be useful for tissue engineering.
Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
Authors: Xiaoshuai Li , Qiushi Wang , Rui Wang 
Published: September 2022

In the past 10 years, gene-editing and organoid culture have completely changed the process of biology. Congenital nervous system malformations are difficult to study due to their polygenic pathogenicity, the complexity of cellular and neural regions of the brain, and the dysregulation of specific neurodevelopmental processes in humans. Therefore, the combined application of CRISPR-Cas9 in organoid models may provide a technical platform for studying organ development and congenital diseases. Here, we first summarize the occurrence of congenital neurological malformations and discuss the different modeling methods of congenital nervous system malformations. After that, it focuses on using organoid to model congenital nervous system malformations. Then we summarized the application of CRISPR-Cas9 in the organoid platform to study the pathogenesis and treatment strategies of congenital nervous system malformations and finally looked forward to the future.
Not sorcery after all: Roles of multiple charged residues in membrane insertion of gasdermin-A3
Authors: Korn Viktoria , Pluhackova Kristyna 
Published: September 2022

Gasdermins execute programmatory cell death, known as pyroptosis, by forming medium-sized membrane pores. Recently, the molecular structure of those pores as well as the diversity in their shape and size have been revealed by cryoTEM and atomic force microscopy, respectively. Even though a growth of smaller to larger oligomers and reshaping from slits to rings could be documented, the initiation of the gasdermin pore formation remains a mystery. In one hypothesis, gasdermin monomers insert into membranes before associating into oligomeric pores. In the other hypothesis, gasdermin oligomers preassemble on the membrane surface prior to membrane insertion. Here, by studying the behavior of monomeric membrane-inserted gasdermin-A3 (GSDMA3), we unveil that a monomeric gasdermin prefers the membrane-adsorbed over the membrane-inserted state. Our results thus support the hypothesis of oligomers preassembling on the membrane surface before membrane penetration. At the same time, our simulations of small membrane-inserted arcs of GSDMA3 suggest that the inserting oligomer can be small and does not have to comprise a full ring of approximately 26–30 subunits. Moreover, our simulations have revealed an astonishingly large impact of salt-bridge formation and protein surroundings on the transmembrane passage of charged residues, reducing the energetic cost by up to 53% as compared to their free forms. The here observed free energy barrier of mere 5.6 kcal/mol for the membrane insertion of monomeric GSDMA3 explains the surprising ability of gasdermins to spontaneously self-insert into cellular membranes.
Identification of novel lactate metabolism signatures and molecular subtypes for prognosis in hepatocellular carcinoma
Authors: Guan Qiutong , Pan Jing , Ren Ninghui , Qiao Chu , Wei Minjie , Li Zhenhua 
Published: September 2022

Background: Evidence has shown that lactate, an immune signaling molecule, is associated with hepatocellular carcinoma (HCC) progression and immune suppression. Therefore, identifying lactate metabolism-related molecules is a promising therapeutic strategy to inhibit the development of HCC and overcome chemotherapy resistance. Long noncoding RNAs (lncRNAs) are related to tumorigenesis and metastasis. Hence, verifying the molecular subtypes of lncRNAs related to lactate metabolism will play a critical role in managing HCC.Methods: Based on HCC data in The Cancer Genome Atlas (TCGA), lactate metabolic pathway-related genes were enriched by gene collection and enrichment analysis (GSEA). Lactate metabolism-related lncRNAs (LM_lncRNAs) were identified by correlation analysis, HCC molecular subtypes were determined using nonnegative matrix factorization (NMF) clustering, and the response of the three subtypes to chemotherapeutics was further evaluated using the Genomic Tumor Sensitive Cell Line (GDSC) dataset. LM_lncRNAs were examined via Lasso-Cox regression analysis to determine prognosis for patients. A Nomagram plot was used to predict patient survival time.Results: Three molecular subtypes of HCC were identified. The survival rate of patients with C1 subtype was higher than that of those with C2 and C3. Additionally, patients with C3 subtype have higher levels of immune cell infiltration and high expression of genes related to immune checkpoints. The GDSC results indicated that patients with C3 subtypes were more sensitive to chemotherapy drugs such as sorafenib and sunitinib. The prognostic risk assessment model consisted of six risk factors (AC034229.4, AC131009.1, MYOSLID, AC008667.1, AC012073.1, AC068025.1) and two protective factors (LINC00402 and AC103858.1). Based on Kaplan-Meier analysis, low-risk HCC patients had a high survival rate, and the receiver operating characteristic curve (ROC), calibration curve, and C-index confirmed good prediction ability.Conclusion: In this study, the molecular subtyping method and prediction model of lactate metabolism-related lncRNAs (LM_lncRNAs) were constructed for the prognosis of HCC patients. This work demonstrated the potential targets of LM_lncRNAs and provided a novel perspective and therapeutic paradigm for future clinical translation.
The ribosome inhibitor chloramphenicol induces motility deficits in human spermatozoa: A proteomic approach identifies potentially involved proteins
Authors: Bisconti Marie , Leroy Baptiste , Gallagher Meurig T. , Senet Coralie , Martinet Baptiste , Arcolia Vanessa , Wattiez Ruddy , Kirkman-Brown Jackson C. , Simon Jean-François , Hennebert Elise 
Published: September 2022

Mature spermatozoa are almost completely devoid of cytoplasm; as such it has long been believed that they do not contain ribosomes and are therefore not capable of synthesising proteins. However, since the 1950s, various studies have shown translational activity within spermatozoa, particularly during their in vitro capacitation. But the type of ribosomes involved (cytoplasmic or mitochondrial) is still debated. Here, we investigate the presence and activity of the two types of ribosomes in mature human spermatozoa. By targeting ribosomal RNAs and proteins, we show that both types of ribosomes are localized in the midpiece as well as in the neck and the base of the head of the spermatozoa. We assessed the impact of cycloheximide (CHX) and chloramphenicol (CP), inhibitors of cytoplasmic and mitochondrial ribosomes, respectively, on different sperm parameters. Neither CHX, nor CP impacted sperm vitality, mitochondrial activity (measured through the ATP content), or capacitation (measured through the content in phosphotyrosines). However, increasing CP concentrations induced a decrease in total and progressive motilities as well as on some kinematic parameters while no effect was observed with CHX. A quantitative proteomic analysis was performed by mass spectrometry in SWATH mode to compare the proteomes of spermatozoa capacitated in the absence or presence of the two ribosome inhibitors. Among the ∼700 proteins identified in the different tested conditions, 3, 3 and 25 proteins presented a modified abundance in the presence of 1 and 2 mg/ml of CHX, and 1 mg/ml of CP, respectively. The observed abundance variations of some CP-down regulated proteins were validated using Multiple-Reaction Monitoring (MRM). Taken together, our results are in favor of an activity of mitochondrial ribosomes. Their inhibition by CP results in a decrease in the abundance of several proteins, at least FUNDC2 and QRICH2, and consequently induces sperm motility deficits.
Large and stable: actin aster networks formed via entropic forces
Authors: Spukti Friedrich Fabian , Schnauß Jörg 
Published: September 2022

Biopolymer networks play a major role as part of the cytoskeleton. They provide stable structures and act as a medium for signal transport. These features encourage the application of such networks as organic computation devices. While research on this topic is not advanced yet, previous results are very promising. The protein actin in particular appears advantageous. It can be arranged to various stable structures and transmit several signals. In this study aster shaped networks were self-assembled via entropic forces by the crowding agent methyl cellulose. These networks are characterised by a regular and uniquely thick bundle structure, but have so far only been accounted in droplets of 100 μm diameter. We report now regular asters in an area of a few mm2 that could be observed even after months. Such stability outside of an organism is striking and underlines the great potential actin aster networks display.
Carbon-encapsulated V2O3 nanorods for high-performance aqueous Zn-ion batteries
Authors: Hao Ziyi , Jiang Weikang , Zhu Kaiyue 
Published: September 2022

Searching for stable cathodes is of paramount importance to the commercial development of low-cost and safe aqueous Zn-ion batteries (AZIBs). V2O3 is a good candidate for AZIB cathodes but has unsatisfied cycling stability. Herein, we solve the stability issue of a V2O3 cathode by coating a robust carbon shell. Strong evidence was provided that V2O3 was oxidized to favorable V2O5·nH2O during charging and the carbon shell could promote the oxidation of V2O3 to V2O5·nH2O. The discharge capacity was increased from ∼45 mA h g−1 to 336 mA h g−1 after V2O3 was oxidized to V2O5·nH2O, indicating a higher Zn2+-storage capability of V2O5·nH2O than V2O3. In addition, the rate-capability and long-term cycling performance are greatly enhanced after coating carbon shells on the surface of V2O3 nanorods. Therefore, the presented strategy of introducing carbon shells and fundamental insights into the favorable role of carbon shells in this study contribute to the advancement of highly stable AZIBs.
Functionalized UiO-66-NH2 by trimellitic acid for highly selective adsorption of basic blue 3 from aqueous solutions
Authors: Wang Tingting , Han Lin , Li Xin , Chen Tianen , Wang Shifeng 
Published: September 2022

A novel metal-organic framework (MOF) UiO-66-TLA (UiO-66-Trimellitic Acid) was synthesized by one-pot method with trimellitic acid as modifier, which can effectively remove the basic dye Basic Blue 3 (BB3) in wastewater. Modification with carboxyl groups facilitates the adsorption of the cationic dye Basic Blue 3. The adsorption of BB3 by the modified UiO-66-TLA was significantly greater than that of its parent MOF. The adsorption capacity of the modified UiO-66-TLA for BB3 (234.23 mg g−1) was 93.2% higher than that of the original UiO-66-NH2 (121.24 mg g−1), this is closely related to the electrostatic interaction of -COOH in trimellitic acid. UiO-66-TLA was successfully synthesized as indicated by various characterization results. The adsorption kinetics conformed to the pseudo-second-order model, and the adsorption isotherm conformed to the Redlich-Peterson isotherm. This indicates that BB3 is a multi-parameter model of monolayer/multilayer arrangement on the adsorbent surface, and its rate-controlling step is chemisorption. The adsorption process was non-spontaneous and belonged to an endothermic reaction, in addition, it has great adsorption stability and regeneration The interaction of the modified UiO-66-TLA with BB3 was mainly affected by mechanisms, such as electrostatic interaction, π–π stacking as well as the abundant functional groups on UiO-66-TLA surface. These results demonstrate that UiO-66-TLA is an efficient, regenerable, water-stable material for the removal of BB3 in solution, with practical implications, suggesting its potential as a dye adsorbent.
A scoring system developed from a nomogram to differentiate active pulmonary tuberculosis from inactive pulmonary tuberculosis
Authors: Yu Qi , Yan Jisong , Tian Shan , Weng Wujin , Luo Hong , Wei Gang , Long Gangyu , Ma Jun , Gong Fengyun , Wang Xiaorong 
Published: September 2022

PurposeThis study aimed to develop and validate a scoring system based on a nomogram of common clinical metrics to discriminate between active pulmonary tuberculosis (APTB) and inactive pulmonary tuberculosis (IPTB).Patients and methodsA total of 1096 patients with pulmonary tuberculosis (PTB) admitted to Wuhan Jinyintan Hospital between January 2017 and December 2019 were included in this study. Of these patients with PTB, 744 were included in the training cohort (70%; 458 patients with APTB, and 286 patients with IPTB), and 352 were included in the validation cohort (30%; 220 patients with APTB, and 132 patients with IPTB). Data from 744 patients from the training cohort were used to establish the diagnostic model. Routine blood examination indices and biochemical indicators were collected to construct a diagnostic model using the nomogram, which was then transformed into a scoring system. Furthermore, data from 352 patients from the validation cohort were used to validate the scoring system.ResultsSix variables were selected to construct the prediction model. In the scoring system, the mean corpuscular volume, erythrocyte sedimentation rate, albumin level, adenosine deaminase level, monocyte-to-high-density lipoprotein ratio, and high-sensitivity C-reactive protein-to-lymphocyte ratio were 6, 4, 7, 5, 5, and 10, respectively. When the cut-off value was 15.5, the scoring system for recognizing APTB and IPTB exhibited excellent diagnostic performance. The area under the curve, specificity, and sensitivity of the training cohort were 0.919, 84.06%, and 86.36%, respectively, whereas those of the validation cohort were 0.900, 82.73, and 86.36%, respectively.ConclusionThis study successfully constructed a scoring system for distinguishing APTB from IPTB that performed well.